LOCUS       DATIFQ010000001         5326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2298950, whole genome shotgun sequence.
ACCESSION   DATIFQ010000001 DATIFQ010000000
VERSION     DATIFQ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2298950"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VLE22_00005"
     CDS             complement(<1..1213)
                     /locus_tag="VLE22_00005"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HSB12804.1"
                     /translation="MRTILTVYAVVFLFCVVARSQSAGTLRAGFAKMDVTPTEPVMMV
                     GYDMRGAPSEGVHGDDKLYVRALVFDDGRSKVMFVEGDVIMIREHDTWKKRISDATGI
                     PAEHILIGDAHNHAAPSPSAAAETNWERQFGQALMAAVKQAAGNLVPVRIAAGDGTSR
                     VAMNRRHVRPEASESILTFDENARSQSFGKFKVDQPKKIYEPAGVMRLGANPAGPIDD
                     AVQLVRIDTAAGKPLAVMIHYACHGTSLGGRNGKVSAEWMGSMQKYVEQQIPGAGSFY
                     VQGAAGDINPRVVGGLDGYVDNIETTRALGVEIGREVVRVYRTLTPEPSDSAIEVKSR
                     DIRLPVAYRQLFEDFNETTVHVPTTAVRIGDLMWVTFPGELFHAIGKRVKAACPAAHG
                     YLMGYTNGYVGY"
     gene            complement(1287..2813)
                     /locus_tag="VLE22_00010"
     CDS             complement(1287..2813)
                     /locus_tag="VLE22_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12805.1"
                     /translation="MPPTRRGFLAGAGLLTARSAHSAGAIVIDPTPVFEISPHLYMQF
                     MEPLGTTDSSVEAAWDYDTDDWRKDFVAVVRDLSPDVIRWGGLFSRYYKWREGVGEPD
                     KRPWMRNYVWGGKETNRVGTLEFVGLCRAVSAEPLYCVNFLGDGDARYQRAREGDRWG
                     DAREAADWVSYCNDPDNRDRKSHGTSGPLNVKLWQIGNETSYGRNSFSRDEAILHTAE
                     FARAMKQRDPSIRLIGWGDKSRIQEEGLWAGEVLKRAGEHLDYVAIHLMGQSPRRPDT
                     VLRGLRYQRAPERAWEELLELSNNVESRIAELEEVVKPYAPRIGIAVTEGHLGLSPHN
                     AHPILAEWLSAVYHARSMNIYQRHGRYVRIATGADFEGVRWTVNAVMLQVPRGISYLM
                     PAGSIMRLFKRHNGKHGVTVTSAPSDLDIAASISEKRLYLHVANIALSRPVMASFSIE
                     GRRITGGRVFEIAPDDPRQAVSQDQPDVFAPREKQIPAQAAAWTFPKASVSVIELDLA
                     "
     gene            complement(2840..4579)
                     /locus_tag="VLE22_00015"
     CDS             complement(2840..4579)
                     /locus_tag="VLE22_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HSB12806.1"
                     /translation="MKPTHVLIFLLTLLSLPVHAADIEVWAAPAIYKVRPDQPSQNSN
                     LVWNAKTKTISVAGAKNEHLPFQVVVSTPPPPTRYHQAASGFFVEASDLVSSNGRISR
                     DRIRLYVQHAVLCYGKSSPIGETGFWPDALAPLTDPFSMAAEFRYFVKNRAIWIDVVT
                     PADVPAGDYAGNIRVTHNGQPVNELKLKLKVFDFALPAETHLITHMGISARQLAVTHN
                     VPPGSPAAKELLRKYHAFLYENRMEPWFNEGLQPEIVERGDDVTLKFDAQSYDLYMNR
                     WKTRRVILEAVPSELARGAREPEFSEATARRVRSYLRQTADYYRKNGWLERLVFNSPI
                     DEPNTAQAYEDTRKWAKLVHEAAPGVPFLVTEAPVTDDPAWGALNGYANNFSVHGNEL
                     NRLEVKEAIHREQARGGEITWYISCDQVYPQPNYFIDAPAMDPVMVPWITLRYGMNGV
                     LYWAFNFWSQTPDPWLDPVTYLSGFLCSNGGILNGEGSLLYPGNRVRRYTGQKNVDGP
                     VSSIRFELLREGIEDYEYLWMLKSLGDQKSADEIVKSMVVDVSAFSRNVEELFAVRER
                     MAKRIEQLARNKR"
     gene            4709..>5326
                     /locus_tag="VLE22_00020"
     CDS             4709..>5326
                     /locus_tag="VLE22_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12807.1"
                     /translation="MKRRELLTGSLSAVAGGPLMARAFAGTQEPAPCAPCAPAPFPAV
                     ASPGAKLRIKPIMTNMIHSGVWEGPCRFNVVTVEKEKENVARGYANWQKVLRSGEHTF
                     GAGAEVMEPSLVTFNEDFTIPAAAFAELDRDARSADAFFIAPQGSSNAAFDLVHRFRK
                     PGILFGINCRTVDVAAYAKTQGDEMLVVEDNEELRSTIDLLRARKI"
BASE COUNT         1016 a         1559 c         1704 g         1047 t
ORIGIN      
        1 gatagcccac gtacccgttg gtgtaaccca tcaggtaccc gtgtgcggcg gggcaggccg
       61 ctttgacccg cttgcctata gcgtggaaca actcaccggg gaacgtaacc cacatcaagt
      121 cgccgatgcg gacggccgtt gtcggcacat gcacggtggt ctcgttgaag tcctcgaaca
      181 gctgcctgta ggccaccggc agccgaatat cccggctctt gacctcgatc gcggagtccg
      241 acggttccgg tgtaagcgtc cggtagacgc gcacgacttc acgtccgatt tcgacgccga
      301 gtgcgcgcgt ggtttcgatg ttatcgacgt agccatcgag accgccgaca acacgcgggt
      361 tgatgtcgcc cgcggctccc tgaacataga aagagccggc gcctggaatc tgctgttcga
      421 cgtacttttg catgctgccc atccactcag cggatacctt gccgttcctg ccgccgaggg
      481 atgtgccatg gcaggcgtag tgaatcatga cagcgagcgg cttgccggcg gccgtgtcga
      541 tgcgaactaa ctggacagcg tcgtcgatag gtccggctgg attggctccg agccgcatca
      601 cgccggcggg ttcgtaaatc tttttcggct ggtcgacctt gaacttgccg aaagactggc
      661 tacgggcgtt ttcgtcgaag gtgaggatcg attctgaggc ttccgggcgg acatgccggc
      721 ggttcatcgc cacccgcgac gtgccgtcgc cggcggcgat cctgaccggc acgaggtttc
      781 ccgccgcctg cttgacggcc gccatcaggg cttggccgaa ttgccgttcc cagttggtct
      841 cggcggctgc ggaaggcgaa ggggccgcat ggttgtgggc gtcaccgatg agaatgtgtt
      901 ccgccggaat tccggtggcg tcggagatcc ttttcttcca ggtgtcgtgc tcgcgaatca
      961 tgatgacgtc gccttcgacg aacatgacct tggagcggcc gtcatcaaag acaagcgcgc
     1021 gaacgtagag cttgtcgtcg ccgtggactc cctcggaggg cgcccctcgc atgtcgtagc
     1081 cgaccatcat gaccggctcg gtcggggtaa cgtccatctt ggcgaatccg gcgcgaaggg
     1141 ttcccgctga ttgggagcgg gccacaacgc aaaagagaaa cacgactgca tataccgtca
     1201 ggatcgttct catgacattc ctcacgctct tgagaagcat acgacaaatg ccggggagcg
     1261 gcggacggac gttgagccga gcgcattcag gcgagatcga gttcgatcac gctcacggat
     1321 gccttgggga acgtccaagc ggcggcctgc gctggaatct gtttttcgcg tggagcgaag
     1381 acgtccggct ggtcctggct gacagcctga cggggatcgt cgggagcaat ctcaaacacc
     1441 ctgccgccgg tgattcggcg gccctcgatg gaaaacgacg ccatcaccgg gcgggacagg
     1501 gcgatgttgg ccacatgcag atagaggcgt ttctccgaga tgctggcggc gatatcgagg
     1561 tccgagggcg cggatgtgac ggtaactcca tgcttcccgt tatgacgttt gaacaggcgc
     1621 ataatcgagc cggcaggcat caggtagctt atgccacgcg gaacctgaag catgaccgca
     1681 ttgactgtcc agcggacgcc ctcgaagtcc gcgccggtgg cgatgcggac gtatcgacca
     1741 tggcgctgat agatgttcat cgacctggcg tgatagacgg ccgacagcca ctccgcaagg
     1801 atcggatgcg cattgtgggg gctgaggcca agatggccct cggtgaccgc tatgccgatg
     1861 cgcggcgcat acggcttgac cacctcttcg agttcggcga ttcgcgattc gacattgtta
     1921 gagagctcga gaagctcctc ccatgcccgc tcgggagcgc gctggtacct gagaccgcga
     1981 agaaccgtgt ccggacggcg cggcgactga cccatcagat gtatggcaac gtaatccaga
     2041 tgttctccgg cccgcttcag cacttcgccg gcccacagac cctcttcctg gatcctggac
     2101 ttgtcgcccc agccgatcaa ccggattgag ggatcccgct gcttcatggc gcgggcgaat
     2161 tcggcagtat ggagaatcgc ctcgtcccga ctgaagctat tccggccgta ggatgtctca
     2221 ttaccgatct gccagagttt gacattcaag ggtccggatg taccgtggct tttgcggtca
     2281 cggttgtccg ggtcattgca ataagacacc caatccgcgg cctcacgggc gtctccccac
     2341 cggtcaccct cgcgcgcccg ctggtatctg gcgtcaccat cgcccagaaa gttcacacag
     2401 tagagaggct cagcgctcac tgcccggcac agcccaacga actcgagtgt accgacgcgg
     2461 ttggtctctt ttccgcccca gacgtagttg cgcatccatg gccgcttgtc cggctcaccc
     2521 actccctcgc gccacttgta gtagcggctg aacagcccgc cccaacggat cacgtccggg
     2581 ctgaggtccc gcacgacggc gacgaagtct ttccgccagt cgtcggtgtc gtaatcccag
     2641 gcggcctcga cggaactgtc ggtggtaccg agcggctcca tgaactgcat gtagagatgc
     2701 ggggagattt cgaaaaccgg tgtgggatcg attacgatcg caccggcaga gtgagcgctg
     2761 cgcgcagtca ggagtccggc gccagcgaga aaaccgcgcc ttgttggtgg catggctaga
     2821 agcctccttc aggaccctgt cagcgcttgt tcctggcaag ctgttcgatt cgctttgcca
     2881 ttctttcgcg aacggcgaag agctcttcga cgtttcggga gaaggcactg acatcgacga
     2941 ccatgctctt tacgatttcg tcggcggact tttggtcacc gagagacttg agcatccaga
     3001 gatactcgta gtcctcgatt ccttcccgga gcaactcaaa gcgaatcgaa gagacgggac
     3061 catcgacgtt tttctgtccg gtgtaccgcc gcacacggtt gccgggatac agcagggaac
     3121 cctcaccgtt gaggatgcca ccgttgctgc acaagaaccc gctgaggtag gtcaccgggt
     3181 caagccacgg gtcgggcgtt tgggaccaga agttgaacgc ccagtacaga acgccgttca
     3241 ttccgtaacg caacgtgatc catggaacca tgacggggtc catcgccggc gcgtcaatga
     3301 agtagttcgg ctgggggtaa acctgatcgc aggagatata ccaggtgatt tcgccgcccc
     3361 gggcctgctc ccggtggatg gcttccttca cttctaggcg gttcaattcg ttgccgtgca
     3421 cggagaaatt gttggcatag ccgttgagcg cgccccacgc cggatcgtcc gtcaccgggg
     3481 cttccgtaac taggaaaggc acgccggggg ccgcctcgtg gacgagcttc gcccacttgc
     3541 gggtgtcttc ataagcttgg gcggtgttag gctcgtcgat cggactgttg aagacgagcc
     3601 gctcgagcca accgttcttt cggtagtagt cggcagtctg ccgaaggtac gatctgacgc
     3661 ggcgggcggt tgcctctgag aattcgggtt cgcgggcgcc gcgtgccaat tccgatggga
     3721 ccgcttcgag gatcacccgg cgggtcttcc accgattcat gtaaaggtcg taagactgcg
     3781 cgtcgaactt cagggtgaca tcgtcgccgc gctcgacaat ctcaggctga aggccttcat
     3841 tgaaccaggg ctccatgcgg ttttcgtaga gaaacgcgtg atacttccgg agcagttcct
     3901 tcgccgctgg ggagcccggc gggacgttgt gagtgacggc gagctggcgg gcggagatac
     3961 ccatgtgagt gatcagatgg gtttcggccg gaagcgcaaa gtcgaaaacc ttgagtttga
     4021 gcttcagctc gttgaccggc tggccgttgt gcgtgacgcg gatgtttccg gcgtagtcgc
     4081 cggcggggac gtccgcgggc gtgacaacat cgatccagat ggctcggttt ttcacgaagt
     4141 agcggaactc ggcggccatg ctgaacggat ctgtcagagg agcaagcgcg tccggccaga
     4201 agccagtctc gccgatagga ctggacttcc cgtagcagag cacggcgtgc tggacgtaga
     4261 gcctgatccg atcgcgcgaa atccggccat tcgacgacac gaggtcgctt gcctcgacaa
     4321 aaaagccgga tgccgcctga tggtagcggg tcgggggagg aggagtggac acaacgactt
     4381 ggaaagggag gtgctcattc ttcgcgcccg caacggaaat cgtcttggtc tttgcgttcc
     4441 aaaccaggtt ggagttttga gagggctgat cgggccggac tttgtagata gccggggcgg
     4501 cccaaacctc gatatcggct gcatggactg gcaggctcaa cagagtcaga agaaagatga
     4561 gaacgtgggt aggcttcatg gcggctgatt cctttcgctg gcaagccctc ataatcgcac
     4621 ggtgtgtcac gggtggagga acctcacggc gaatgggaaa tggcgggccg acgggtgtat
     4681 tatgcggggt cgatggaggg cataagaaat gaaacgacgt gaactgctga ctggaagtct
     4741 ctccgccgtg gccggcggcc ccttgatggc gcgagcgttc gccgggacgc aggaacccgc
     4801 tccctgcgct ccatgcgcgc cggcgccctt cccggcggtt gcttcgccgg gagcgaagct
     4861 gcgcatcaag ccgatcatga ccaacatgat ccattccggc gtttgggaag ggccgtgccg
     4921 gttcaacgtg gtcaccgtgg aaaaggagaa ggaaaacgtc gctcgcggat acgcgaactg
     4981 gcagaaagtc ctccggagtg gcgagcacac cttcggcgcc ggcgccgaag tgatggagcc
     5041 gtcgctcgtg accttcaacg aggacttcac gattccggcc gcggcgttcg ctgagttgga
     5101 ccgcgacgca cgcagcgccg atgcgttttt tatcgcacca caggggtcgt cgaacgcggc
     5161 gttcgacctt gttcaccgct tccggaaacc cggcatcttg ttcggaatca actgccggac
     5221 ggtcgacgtg gcggcctatg ccaagacgca gggcgacgag atgctggtgg tcgaggacaa
     5281 cgaggaactc cggagtacga tcgatctgct gcgggcacgc aagatc
//
LOCUS       DATIFQ010000002         8698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1916028, whole genome shotgun sequence.
ACCESSION   DATIFQ010000002 DATIFQ010000000
VERSION     DATIFQ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916028"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VLE22_00025"
     CDS             complement(<1..806)
                     /locus_tag="VLE22_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSB12808.1"
                     /translation="MVLRFLLFMVPVFSAIGQEWPYYGGDSGGTKYSPLKQINRSNVG
                     RLKVAWTYHTGDISDGKKWPVKSAFEATPLVTDGVLYFTTPFCRLIALDAETGREIWK
                     FDPGLDKEQPYNLFINRGAALWTDGKDRRLFFGTLDGRLFAIDARTGRPVETFGGKGW
                     IDLRKGVADDFPDRGYGLTSPPTIYKNLVVCGSLTADGEARGPSGDVRAFDANTGALA
                     WRFHVVPRPGEYGHDTWDGESWKDRGAVNAWSILSVDEERGIVYLPLTSPS"
     gene            complement(826..1305)
                     /locus_tag="VLE22_00030"
     CDS             complement(826..1305)
                     /locus_tag="VLE22_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB12809.1"
                     /translation="MQTNAKPSVMIVDDEDMVITSLRAYLRLETDYNVEGFTDPEDAV
                     KRLEANQVEVVVTDYLMPKMNGIQLLAHAKQLQPEATRILLTGHADKQSAIQAINDVG
                     LYQYLEKPWENAQLLLVIQNGIERTQLLRSLESKISELDMAHSNLKDVQSRLLRAFM"
     gene            complement(1325..2155)
                     /locus_tag="VLE22_00035"
     CDS             complement(1325..2155)
                     /locus_tag="VLE22_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB12810.1"
                     /translation="MPEGHELAHLTPEGRLRTELDEANRQVAELYKLASLGRLLASVV
                     HEINTPISSILTNNEVVLRSLEKLRALLAKPDNAVSDPKVSEIVETIQSVSLVDKIAC
                     ERISSVIRSLRTFSRVDESDLRKVDLHEILRDSLKLGSCEFRRRVEVETEFGELPLVE
                     CYPHLLNQVFINLLINAGQAIEGKGKVTVKTSMEGDQVHVAIADTGSGIKPEDRPKIF
                     APGFTTKPLGVGTGLGLAISRKIVVETHGGSIDFETQEGVGTTFHVRIPVRRRETGGS
                     "
     gene            complement(2179..2601)
                     /locus_tag="VLE22_00040"
     CDS             complement(2179..2601)
                     /locus_tag="VLE22_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSB12811.1"
                     /translation="MAELNIVEKVIALEAVELLQSLTPEQLSRIAAIAKEVQYLPDKV
                     ILQNEQPMDALYVILDGAVELSRAGQRLHVARQNEVLGAWALFDEEPMPVIARTLEDT
                     KLLRIGRDDFYDLLSDNLEIAASIFSTLVKRFRKLIEP"
     gene            complement(2615..5353)
                     /locus_tag="VLE22_00045"
     CDS             complement(2615..5353)
                     /locus_tag="VLE22_00045"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HSB12812.1"
                     /translation="MRKIRQIWRSAFDVREGERLRTLFMSLYLLAVLFAYYIVKPVSR
                     AMFVDHFDIDRLPYLYILIAIVGGVLATLYTRVAVSASLNAAVAWATAISIVCLVGLW
                     WLVQLKLPWMLYVFNIWASLFGVVLVSQGWLVAANVFNTREAKRLYGLVGLGAVLGAW
                     FGSAFTKFTVKLIGVENLLLACAALVIVSYVLYRLTVAQKGVSLAGAKAVEVEEARFT
                     SRDIVKDIGRHRHLQVITAIITITFIVDVMVEYQLQAAGKLVYKGAEAYSAFLSTYYF
                     YQNVATFVLQLFFTGALVRKLGVGGTLQLMPVTIAGASLVSALWPGTQTAQVARLVEA
                     TDRYTFNRTGMELLYLPLPAELRNRTKAFVDIFVDRMGRGLGGMLLIALTWAGLTDLS
                     RLPWVVVVFCGVWVLLSIRAQHEYVRTVRRRLEARRFDLESARVTVGDPATLRLLEQA
                     ARGGNVRQACYALSVLGEAPGYEIRPLLSELVESTLPEVRSEVYRLARSAGFDGLIGW
                     AQAELPTAKHAGVRDSAVRYLLAVSPERTSTAREFLNSDNLRLTESTLAAIGENAGEF
                     REVVTHEWCARGITDPDPERRRLAAIAIGVCGEDGREFLRPLIDDPAPSVAAAAFRTA
                     GSLQNRSYLHDIVPKLADPRLRGAAIEALAAYGTRIVGTLSDFLEDDHVSPAIRKQIP
                     RVLRAIQDQRSVDVLIRSINQPNLAVRAAVLKALNRLRASAPSFNYGDVFVTRQILNE
                     ARLYFELSAALAPFRGHKGPRTAAGLLAVSIEERLNQTLERLFRLLGLRYPPKEMYAA
                     YLALHSRKHEQYAAALEFLDNVLDRELKRILLPLLDDGGQLMERGRELFGVEIRTPEE
                     AIRELIRSRDSWLVVCAMAAAGELRMRGLAAEITEAAKGAGAEVCQVAQAAQFALA"
     gene            5455..6882
                     /locus_tag="VLE22_00050"
     CDS             5455..6882
                     /locus_tag="VLE22_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB12813.1"
                     /translation="MPELYQRLLAYLLPGKAEEEDGFRLRIEKLSRQGLKVVAIVEMF
                     APLFMLAAQYFVTPEISTWEPRIWQSVAVMCVGALTHLVVRPQRRGFPYRLAGWLSGF
                     ATGIVLITSSLLLFDLGVTADHYISGQVTSVLLVGVGALPLLPWHTLSLGLAMWTYYV
                     VAGPLAVRWGFLTVIDPDGTHSIFILMITFLSTALSALLYSERLANYRAHQEALASSE
                     SLCRAQSHLLLSENAASLGRLAAAISHELNNPVGALRSAVDTLLLLGARQATTQGEDQ
                     QRLVLLQADLRRSIYDSTARLQQTVGRLQRFTNLDNADIRPADINALVNDVVALQEHE
                     IRGSIHLELDLQPMPPLLCRPQQLSAVLSSILHNAMQATVNGDGRIRVSTREADLHIE
                     VSVRDNGRGLSPGELATVFDPGFKITGGRVSTGNWSLFSARQIVREHLGDILIESEEG
                     RGTTVSVRIPRLTEAELERGAGARS"
     gene            6894..8063
                     /locus_tag="VLE22_00055"
     CDS             6894..8063
                     /locus_tag="VLE22_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB12814.1"
                     /translation="MTQQPKVLVIDDEEAARYGITRALTHQGYSIEEAADGIIALTAI
                     QQFQPDVVVSDINMPGIDGLTLLRRLNEQREPPLVVLITAYGSERIAIEALRAGAHDY
                     ISKPFDVEELRLVVAGAVEKKRLLRELAASQTARVHAEKLASLGRLVAAVAHEINNPL
                     GALLSSFDTIRRAAARIQDWCGRQPAETSEEVRGALEALAAVTAQSQSACRRIDAVVT
                     NLRRFAQLDRADVQRTSVHSGIEDTLHLLRYEFGEGVRVETEFGEIPEIDCAPRDLNQ
                     LFMNLLLNSKRAIDQAGRPGVIRIRTWPAGDTLKIQISDNGCGIAPENIDNVFDPGFA
                     TQGGRVGTDLGLPICQQIARAHNGRIDVESRAGEGTHFTITLPVRATEGTSAAAF"
     gene            8047..>8698
                     /locus_tag="VLE22_00060"
     CDS             8047..>8698
                     /locus_tag="VLE22_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12815.1"
                     /translation="MQPPSDVPWLVLFAVGAALHFFLAAVVIQKRSSATADRLVLAAL
                     LAVGLWHGSRAGSLLHRTLTEQESENSVLLGLGVAVLVAALALVVHAGVAWAGLTNWL
                     AAPVYALTPAAWWLERSGHQEIYRLWVVLTLVWTVALCIRASRGGAEVHERRFFNLFA
                     AALSLILLAAAAFGPDSSSMVLASLLPTAVFARYVYRYNLFGLIIGQRIVFALKLGI"
BASE COUNT         1684 a         2736 c         2520 g         1758 t
ORIGIN      
        1 gatggcgacg taagaggcag atagacgatt ccacgctctt cgtccacgct caggatcgac
       61 caggcattca ccgccccgcg gtctttccag gactcgccat cccatgtatc gtggccgtac
      121 tctcctggcc gaggcactac atggaatcgc catgccaggg ccccggtatt cgcatcgaac
      181 gcccgcacgt caccgctagg cccccgcgct tcgccgtccg ccgtcagcga tccgcagacg
      241 acaaggtttt tgtagattgt cggcggcgaa gtcaacccgt aaccgcgatc cgggaagtcg
      301 tcggcaacgc ccttgcggag gtcgatccag cccttcccgc cgaaggtttc cacggggcgt
      361 ccggtgcgcg catcaatcgc gaaaagcctc ccgtcgagcg ttccaaagaa aaggcgcctg
      421 tccttgccat cggtccagag tgccgctccg cggttgatga acaggttgta tggctgttct
      481 ttgtccagtc ccggatcgaa cttccagatt tcccgccctg tttccgcatc cagcgcaatc
      541 aaacggcaga acggcgtcgt gaagtacagg acgccgtcgg tcacgagcgg cgtcgcctca
      601 aaggcgctct ttaccggcca ttttttgcca tcgctaatgt cgcctgtatg atacgtccac
      661 gcgaccttca accggcccac gttcgatcgg ttgatctgtt tcagcggcga atacttcgtc
      721 ccgccggagt cgccgccgta atagggccac tcctgtccta tggcggagaa gaccgggacc
      781 ataaacaaca ggaatcgcaa aaccatggtg tgaagggcgt atccgctaca taaatgcacg
      841 cagcagacgg gattgcacgt ccttcagatt ggagtgggcc atgtcgagct ccgagatttt
      901 cgactcgagg ctccgcagaa gctgtgtccg ctcaataccg ttctgaatca ccagtagaag
      961 ctgagcgttc tcccagggct tctcgagata ctggtacaag ccgacgtcgt tgatcgcctg
     1021 aatcgcgctt tgcttgtcgg cgtgaccggt gagcaggatg cgcgtcgcct ccggctgaag
     1081 ctgtttcgcg tgagccagca actgaatgcc gttcatcttc ggcatgaggt agtctgtgac
     1141 cacaacctcg acctggttgg cctcaagacg cttcacggca tcctcggggt cggtgaagcc
     1201 ctcgacgtta taatctgtct ccaggcgaag ataggcccga aggctggtaa tgaccatgtc
     1261 ttcgtcgtcg acaatcatta cggacggttt tgcgtttgtc tgcactgcgc gagctcctgg
     1321 aatcttagct gccgcccgtt tccctccggc ggactggtat tctgacgtgg aaggtggtgc
     1381 caacaccttc ctgggtctcg aagtcgatcg agccgccgtg tgtttccact acgatctttc
     1441 tggaaatcgc cagaccaagc cccgtgccga cccccagtgg tttcgtcgtg aaaccgggtg
     1501 cgaaaatctt gggcctgtct tccggcttga tcccgctgcc cgtgtcggca atcgccacgt
     1561 ggacctgatc tccctccatg cttgtcttga ccgttacctt gcccttcccc tctatggcct
     1621 gtcccgcgtt gatgagaagg ttgataaata cctggttcag caaatgcggg taacactcca
     1681 caaggggaag ttcgccgaat tctgtctcca cctcgacgcg ccggcgaaat tcacagctac
     1741 ccagcttgag ggaatctcgg aggatctcat gcaagtcaac ctttcgcaga tctgactcgt
     1801 ctacccgcga aaacgtcctc aggctgcgaa ttaccgacga gatacgctcg cacgcaatct
     1861 tgtccaccag tgaaacactt tggatggttt cgacaatctc cgaaaccttc gggtcggaaa
     1921 cggcgttgtc tggttttgcc agaagtgcac gaagtttctc aagcgagcgc aggaccacct
     1981 cgttgttcgt aagaatcgaa cttatgggcg tgttgatctc gtgaacgaca cttgccagca
     2041 gtctgcccaa cgaggccaat ttgtacagtt ccgccacctg ccggttggcc tcgtcgagtt
     2101 ccgtgcgcaa ccggccctcc ggggtcagat gtgccagttc gtgtccttcg ggcattcagg
     2161 cctggctcct gtataccatt atggctcaat cagtttgcgg aaacgcttga cgagagtgga
     2221 gaagatcgag gccgcgatct ccaggttgtc ggatagtaag tcatagaagt catcccgccc
     2281 gatccggagc agtttcgtat cttcgagtgt ccgggcaatc accggcatcg gctcttcgtc
     2341 gaacaaagcc cacgcgccga gcacctcgtt ctgacgggcc acgtgcagcc gctgtccggc
     2401 ccggctcaat tccaccgccc cgtcaagaat cacgtacaag gcgtccattg gctgctcgtt
     2461 ctggaggatc accttgtcgg gtaggtactg cacttctttg gcgatcgccg cgatgcgtga
     2521 caactgctcc ggtgtcaggc tctggagcag ttccaccgcc tcgagtgcaa tgactttctc
     2581 gacgatattg agttccgcca tcgttggctc tccttcaggc cagcgcgaac tgggccgcct
     2641 gggcgacctg acagacttcg gctcccgcgc ctttcgccgc ttcggtgatc tccgcggcaa
     2701 gaccgcgcat gcgcaattcg ccggcggcgg ccatcgcaca gaccaccagc catgaatcgc
     2761 gagagcgaat caattcccga atcgcctctt ccggtgttcg tatctcgacg ccaaacaatt
     2821 ctctgcccct ctccataagt tgaccgccat cgtcgaggag gggcagcaag atccgtttca
     2881 attcacggtc caggacgtta tccaggaatt ccagagcggc ggcatattgc tcgtgtttcc
     2941 ggctgtggag cgcgagatac gccgcgtaca tctctttggg aggataccgg agaccaagca
     3001 gccggaatag cctctccagg gtctggttga gccgctcttc gatactgaca gccagcaagc
     3061 cggcggcagt acgcgggccc ttgtgaccgc ggaacggcgc taatgcggca cttagttcga
     3121 agtacagtcg tgcctcgttg aggatctgtc tagtgacgaa aacgtcgccg tagttaaaac
     3181 tgggcgccga tgctctgagc cggttcaaag ccttcagcac ggcagcccgc accgcaaggt
     3241 tgggctgatt gatcgagcga atcagcacat ccacactccg ctgatcttgg atcgcccgaa
     3301 gcactcgcgg gatctgcttc cggatcgcag gggaaacgtg atcgtcttcg agaaagtcgc
     3361 tcagggtacc gacgatgcgt gttccgtaag cggcgagcgc ctcgatcgcc gcaccgcgca
     3421 gtcgcgggtc ggccagcttc ggaacaatat catgcaggta ggaacgattc tgaagggatc
     3481 cggcggtgcg gaaagcggcc gctgcgacgg atggcgctgg atcgtcgatt aatggtcgca
     3541 ggaactcccg tccgtcctcg ccgcacacac cgatcgcgat cgcggccaga cgccggcgct
     3601 ctggatcggg atcggtaatc cctcgggcgc accattcgtg cgtcactacc tcgcgaaatt
     3661 cgcccgcgtt ttccccgatc gccgccagag tgctctcggt cagccggagg ttgtcgctgt
     3721 tcaagaattc tcgggctgtc gacgttcgtt cgggtgagac cgccagaaga tagcgtaccg
     3781 ccgagtccct cacgccagcg tgtttggcgg taggcaattc cgcctgagcc cacccgatga
     3841 gtccgtcgaa cccggcggac cgggccagac gatacacctc actccgcact tccggcagcg
     3901 tgctctccac aagttcagaa agcaggggcc tgatctcgta gcccggcgct tcccccaata
     3961 ccgacagcgc gtagcatgcc tgcctcacgt tgccgccacg ggccgcttgc tccaacaaac
     4021 gcaatgtcgc cggatcgccg accgtaactc gagcgctttc caggtcaaac cgccttgcct
     4081 ccagtcgccg gcggaccgtt cgcacgtact cgtgttgcgc ccgaattgac aacaagaccc
     4141 aaacaccaca gaagacaacc acgacccaag gcagccttga gaggtccgtg agacccgccc
     4201 atgtcagcgc aatcaggagc ataccgccga gaccccgccc catgcggtcg acgaatatgt
     4261 ccacgaacgc cttcgtccgg ttcctaagtt ctgccggcag cggcagatag agcaactcca
     4321 tgcccgtgcg gttgaaggtg taacggtccg ttgcctcgac aagcctggcg acctgtgccg
     4381 tttgcgtgcc cggccacagc gcggaaacca aggaagcccc agcgatcgta accggcatca
     4441 attggagcgt cccgccaact ccgagcttgc gcaccagcgc tccggtgaag aagagctgca
     4501 gcacgaacgt agctacgttc tggtagaagt agtacgtact caagaacgcg gagtaagcct
     4561 ccgccccctt gtagacgagc ttgccggccg cctgcaactg gtactcaacc attacgtcga
     4621 caatgaacgt gatcgtgatg atcgccgtga tcacctgcag atgccggtgt cggccgatat
     4681 ccttgacgat gtcccgagac gtgaaacggg cctcctcgac ctcgaccgcc ttggcgccgg
     4741 ctagagaaac tcccttctgc gcgaccgtca gccggtacag cacgtaggac acgatcacca
     4801 gcgcggcgca ggcaagcagt aggttctcca ctccgatcag cttcaccgtg aacttcgtga
     4861 acgcgctccc gaaccaggcg cccaagaccg cgcctaaacc aaccagaccg taaaggcgct
     4921 tcgcctcccg tgtattgaag acgtttgccg ctaccagcca gccctgtgaa acaagcacaa
     4981 ccccgaaaag gctcgcccag atgttgaata cgtaaagcat ccatggcaac ttcagttgaa
     5041 ccagccacca caagccgacc agacagacaa tcgagatcgc cgtcgcccac gctaccgcgg
     5101 cgttcagcga cgcgctcacc gcaaccctcg tgtacaaggt ggcaagcaca cccccgacaa
     5161 tcgcaatcag aatgtacaaa tagggcagcc ggtcgatgtc aaaatgatcg acgaacattg
     5221 ctcgcgatac gggtttgaca atgtagtagg cgaacaggac agccagaagg tagagggaca
     5281 tgaagagcgt ccgcagacgc tcgccttccc tcacatcgaa cgcactgcgc cagatttgcc
     5341 tgatcttacg catgaggaag cccactcacc ggcggcgatg accggaatcg tctgatgcgg
     5401 tagtgtagca caactctttc ggcgagccgg tgcgtcataa tttgaagata cgggatgccc
     5461 gaactgtacc agcgccttct ggcttatctc cttcctggga aagcggagga ggaagatggc
     5521 tttcggcttc ggatcgaaaa gctgagccgc caggggctta aggtcgtcgc catcgtcgag
     5581 atgttcgcgc cgctattcat gctggctgcc cagtattttg tgacgccgga gatatccacc
     5641 tgggagcccc gcatttggca gtccgtcgcg gtaatgtgcg tcggcgccct gacgcatctg
     5701 gtggtgcggc cgcagaggcg cggttttccg taccgccttg caggttggct ctcgggcttc
     5761 gccactggaa tcgttctgat cacgagttcc ctgcttctct tcgacctcgg cgtgacggcc
     5821 gaccactaca tctcagggca ggtgaccagc gtgctactgg tcggagtcgg agcacttcct
     5881 ttgcttccct ggcacacgct cagtctcggc ttggcaatgt ggacatatta tgtcgtagcg
     5941 ggcccattgg cggtgcgttg gggcttcctc acggtcatcg accctgacgg gacccacagc
     6001 attttcatcc tgatgatcac gttcctcagc accgccctct ccgcgctgct gtattccgag
     6061 cgcctcgcca actatcgggc acaccaggag gcgctggcga gttccgaaag cctgtgccgg
     6121 gcgcagtccc accttctcct ttcggagaac gctgcttcgc tgggccgtct ggccgccgcg
     6181 atcagtcacg aactcaacaa tccggtgggt gctttgcgga gcgccgtcga cacgcttctg
     6241 ttgctcggcg cccggcaggc cacgacgcaa ggagaggacc agcagcggct tgtcctgctt
     6301 caggccgacc ttcgacggtc catctatgat tcgactgccc ggcttcaaca gactgtcggc
     6361 cgcctccagc gcttcaccaa cctcgacaat gccgacatcc ggccggcgga tatcaacgcg
     6421 ctagtcaatg acgttgtcgc cctgcaggag cacgagattc gcggttcgat tcatctggaa
     6481 cttgaccttc agccgatgcc gccgctcctc tgccggcctc agcagttgag cgccgtgctc
     6541 tccagcatcc tccataacgc catgcaggcg accgtcaacg gcgacggccg aatccgcgtc
     6601 tcgaccagag aggccgacct ccacatcgaa gtcagcgttc gcgacaatgg gcgcggcctc
     6661 agccctggcg agctggcgac ggtttttgac cccggcttca agattaccgg gggccgcgtg
     6721 tcgacgggta actggagcct gttcagcgcc cgccagattg tcagggaaca tctgggtgat
     6781 atcctgatcg agagcgagga aggtagaggg accaccgtga gtgtacggat tccccggcta
     6841 accgaagcgg aacttgagcg aggcgccggc gcgcgctcct agagaggcgt gctttgacac
     6901 agcagccgaa ggtcctggtc atcgacgacg aggaggccgc tcgctacggc atcacgcgtg
     6961 cgttgaccca tcagggctac agcatcgagg aagcagcaga cggcatcatt gcgcttaccg
     7021 caatccagca gtttcaaccg gacgtcgtgg tctccgacat taacatgccg ggcatcgacg
     7081 gtctgaccct gcttcgccgg ctcaacgagc agcgcgagcc tcccctggtc gtgctgatta
     7141 ccgcgtacgg ctccgagcga atcgccatcg aagcattgcg tgcgggcgcc cacgactaca
     7201 tttcgaaacc gttcgacgtc gaggagctac ggctggttgt cgccggtgcc gtcgagaaga
     7261 agaggcttct gagagagctt gcggcttctc aaaccgctcg tgtacacgca gagaagctag
     7321 cctctctcgg acggctggtc gcggcagtgg cgcacgagat caacaacccg ctcggagcgc
     7381 ttctgagcag cttcgacaca atccggcggg cggccgcgag gatacaggat tggtgcggac
     7441 gccagccggc ggagacctcg gaagaggttc ggggcgcatt ggaagctctg gctgctgtga
     7501 ctgcacagtc gcaatcagcg tgcagacgca ttgacgccgt ggtgacgaac ctgcggcggt
     7561 tcgcccaact ggacagggcg gacgtccagc ggacgagtgt ccactctggg atcgaggaca
     7621 cgcttcactt gctgcgctac gaatttggcg aaggcgttcg agtggaaacc gaattcggcg
     7681 agattccgga aattgactgc gcgcctcgcg acctcaacca gctcttcatg aacctgttgc
     7741 tgaattcgaa gcgggccatc gaccaagccg gtcgtccggg agtgattcgt atccgcacgt
     7801 ggcccgccgg cgacacgctg aaaatccaaa tctccgacaa cggctgcgga attgcgccgg
     7861 aaaacatcga caacgtcttc gatccgggtt tcgcaaccca agggggacga gtcggcaccg
     7921 atctcggtct gcccatttgc cagcagattg ctagggcgca caacgggcgg atcgacgtgg
     7981 aaagccgggc cggagagggg acccatttca cgataactct gccggtgcgg gctacggagg
     8041 gaaccagtgc agccgccttc tgatgtgccg tggttggttt tgtttgccgt tggcgctgct
     8101 ctgcatttct ttctggcagc cgtcgtcata cagaagcgga gcagcgccac cgcggacagg
     8161 ctggttcttg ccgcactcct agctgtcgga ctttggcatg gctctcgtgc gggatcgctt
     8221 ctccatcgaa ctcttacgga gcaggagtcg gaaaactcgg tgttgctggg tctcggcgtg
     8281 gcggtactcg tggccgccct ggcgttggtg gttcatgccg gcgtcgcatg ggccgggctg
     8341 accaactggc tggctgcccc ggtctatgcg cttacccccg cggcgtggtg gctggagcgg
     8401 agcggacacc aggagatcta ccgtctttgg gtcgtgctca cgctcgtgtg gaccgttgcc
     8461 ctgtgcatcc gggcgtcgcg cggcggagct gaagtccacg agcggcgttt tttcaacctc
     8521 tttgccgcag ccctctcgct gatcctgttg gcggcagcgg ctttcggtcc ggattcctcc
     8581 tcgatggtgc tcgcgtcgct cctgcccacc gcggtcttcg ctcgttacgt ctaccggtac
     8641 aacctctttg gactgatcat tggccagcgt atcgtttttg cactaaaact cgggatcg
//
LOCUS       DATIFQ010000003        31611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1341652, whole genome shotgun sequence.
ACCESSION   DATIFQ010000003 DATIFQ010000000
VERSION     DATIFQ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 31611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31611
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341652"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1020
                     /locus_tag="VLE22_00065"
     CDS             43..1020
                     /locus_tag="VLE22_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSB12816.1"
                     /translation="MDYDTFIQKLRAYQDSRILLTAIELHLFNAVGAGAPAEEIAERL
                     DTDPRATEMLLNALVAVEMLKKRDGLFTNTPLAAQYLTAGSPDNERDAWMHSVNQWAR
                     WSRLTSCIRTGKAPVLPEIGARGESWIRAFIAAMHRNAAGRAPQLVETVGASTVARML
                     DIGGGSGAYSIAFALANPNLHAEVFDLPEVLPIAQGHIDAAGLTGRVVARAGDLRRDG
                     FGDGYDLILLSAICHMFGEDGNRDLLRRAYRALAPAGRVVIQDFVLEEDKTTPRMGAI
                     FALNMLVSTHEGNSYSYQEYAGWLNEAGFQDVSRHWPGEPTGLIVGVRG"
     gene            1064..2245
                     /locus_tag="VLE22_00070"
     CDS             1064..2245
                     /locus_tag="VLE22_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB12817.1"
                     /translation="MKITRRHALLGAAAPLIVKRETAFASQANSAVAFGIIGTGGRGR
                     YVGGFMAKDSSAKLVAICDKYPDRIDLGKTEIPGADQAPAFRDYQELLARPDIDAVLI
                     ATPVFLHPEHFEAAVKAGKHVYCEKPAGAGVAGCKRLLKAGDRADTSKTIQFGFQQRF
                     SPEYLTAMSLLRSGRIGDMRLMMSYRMLGGLPPAAFTNPYTGPDEKIRNWGAWVAYSG
                     GPIVEQDCHGVDTLNWFAGDVHPTRAFGTAGQRYPVPYGDWKTDYHDIAFYYPNQLEG
                     WLISIKHTAGYRDVKEQLFGSKGLLETSRSYYKLHGPEGNWRYPNADDLRDRSLIEKR
                     DSAREITIDAVEAFFKSIVEKKPYHMWKTSAESTLTALLGQMAVEKRREVTWDELLRS
                     A"
     gene            complement(2252..3895)
                     /locus_tag="VLE22_00075"
     CDS             complement(2252..3895)
                     /locus_tag="VLE22_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12818.1"
                     /translation="MKRFSLLLAAAAAVNALGVPPDDIWKTARANGAIMDDIFRRTRK
                     MMHGWLAHADSQTLLLPDGLPGLIRGKPNDRSVYTPHNSGADNYPFLITTAFFTEPAL
                     YRGRMMEMLRNEVRFTNVGGGAIPGNLDFKTRELGPPSFFGAGEYCKDGMVPVTELLG
                     RTPWFYRMVDMMEDFMARAPVKTRFGNLPDTGAELNGDVLQALARLIPMTGDPRFLKW
                     AEQIGDAYIEEILPGSNYLPAYGWDFDKKTATETVGLGDHGNEAIVGLVLLQALETEL
                     GGSRAKSYRPVLRKTFDRVLESANPDGFIYRQIRTADLQPVDAALTDCWGYVYASMYA
                     YYQTTGEQKYREAVLRVLKNLAKYRDYDWTKRVNTVDDLADSIEGAIYLVAHEPVPEA
                     LAWIDHSIPKMYPYQQPDGVIERWYGDGNWNRTLLLYAMMKTQGARLKDWAPGVELGA
                     VRSGEDLYISVHSAGTWSGSVCLDYARYRRVLNLKKDYARLNQWPEWYTVDENTLYRV
                     LDPDTGNEEIRLGSELKDGFDLRVAGKTARRVISRAAGK"
     gene            4053..4508
                     /locus_tag="VLE22_00080"
     CDS             4053..4508
                     /locus_tag="VLE22_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12819.1"
                     /translation="MTCSRTPILGVASILLFASLALGNPGKPNFGPSVYGDGKVWGTK
                     GAATLSAPNDNNMQSYDKLFVFVNGAMGQIPVAEAAPGNPMYNGGRWHTHTVVWTQAG
                     LDFHGGTLPVLTSYDEIQFHYGLGHLMITPGSPAGGPPPYFECPLLPVK"
     gene            4642..5292
                     /locus_tag="VLE22_00085"
     CDS             4642..5292
                     /locus_tag="VLE22_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB12820.1"
                     /translation="MRFLLLLALVPPAAAAPASFFGGFVGVSTLSADGAFSTRGNTSA
                     HSQYKPENGPTAMVFAGRHLNDFFAVQLSYGWNRNEVLLAATEAGTTANAYEQGRRNR
                     QHTVIGEGMVFFRKRQSRVRPYLTAGAGVHQLTSKANGRLVLIGNPVLPPAEFSKTGP
                     AFRVAVGIDVFLRGGFAWRYSFSETIQGNPMSKQLSPQGARNLKNFQNLFGFAWYF"
     gene            complement(5318..5653)
                     /locus_tag="VLE22_00090"
     CDS             complement(5318..5653)
                     /locus_tag="VLE22_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12821.1"
                     /translation="MADDLDFKRLASVAEAPYGPERKAPAWLKSRTYTALVQRQQESG
                     PLLSLTGSKQNGRKLCVFEELVQIVPAGARTESAFFCKVCHARLLAEHIENAPIWWPG
                     CPYVRFQNR"
     gene            complement(5646..6302)
                     /locus_tag="VLE22_00095"
     CDS             complement(5646..6302)
                     /locus_tag="VLE22_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB12822.1"
                     /translation="MDPEGGFAPGNRHPDVSGSSATTPAARGDAAVSQGEEAASETEL
                     VHAVLRKDRKATEEFVSRYSDSVYSYLSSRLLPHADVADDCFQQVFLDAWQALPGYRG
                     DSGLKSWLLGIARHKVQDHFRERLRLAQWSEEDAEPAAEESLEASIEREEEQSLVWKM
                     LGTLPENDRILLVWRYWERQSAEEMARQTGKSVKGVERALARAREHFRRRWQSEVQNG
                     "
     gene            6403..7149
                     /locus_tag="VLE22_00100"
     CDS             6403..7149
                     /locus_tag="VLE22_00100"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB12823.1"
                     /translation="MQYRLTAVAVLLVVAAGLSAPNALTQAPAGFVSLFNGRDLSGWT
                     VPEGDNGHWKVVDDVIDYDARSESPKDKNLWSEKEYKDFILRVDWRIKATPYRNKDAR
                     IVMPDGSDKLDENGKVIQLDLPDSDSGVFLRGTGKGQVNIWCWPVGSGEFYGYRTDPK
                     MPAEVHAAVTPKMNADRNIGEWNTFEITVRGNAVTVVLNGKKVIDAAKLPGLPEKGRL
                     ALQHHGSFKDGQWQGPPSLVQFRNIYIKEL"
     gene            complement(7183..8034)
                     /locus_tag="VLE22_00105"
     CDS             complement(7183..8034)
                     /locus_tag="VLE22_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12824.1"
                     /translation="MPVTTISFFARASVAAGLFCTAALGQVVFSENFESGELGGSWEK
                     YSEDPVRGGFETRKEYVHAGTKSYRISAPAFQGEGKTVEGRVLRESDSYIRAWFLPGY
                     DRVYIRWYAKFAENFEGRGMHWCQFWACRPDSPRSVLGGAGRKPDGTDRFIVNVEPRG
                     VASMPPPGQIVFYTYWPDMKQSPDGRYWGNYFYPKEPFWIERGRWYCFEMLVKCNQPG
                     KKDGEQVLWIDGKEILRVDGMRWRDVESLKLNMAMFGNYSSNSQHDRTYWLDDVVIST
                     EYVGPVK"
     gene            complement(8161..8235)
                     /locus_tag="VLE22_00110"
     tRNA            complement(8161..8235)
                     /locus_tag="VLE22_00110"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8201..8203),aa:Cys,seq:gca)
     gene            8614..8994
                     /locus_tag="VLE22_00115"
     CDS             8614..8994
                     /locus_tag="VLE22_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HSB12825.1"
                     /translation="MRPFQRYVGIQFPSWVLTAMVVWGLHHWTGLPRGWAITLLLAYV
                     AKDFLLYPLLRRSYEEDRRTGAEPLIGRTGIAVEELNPAGYVRVRGELWRAEAAGGAV
                     PAGAAVTVVSTDGMTLVVEAQRAH"
     gene            complement(9013..10812)
                     /locus_tag="VLE22_00120"
     CDS             complement(9013..10812)
                     /locus_tag="VLE22_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HSB12826.1"
                     /translation="MKTRVFVIFVLLLTVASALPAAGPPTVAEARAFLDDAETQLMAL
                     SEEAGHASWVQATYIMMDSEILAAKANQRMITATVELAKQSTRFDGVSLPPDLARKMK
                     LLRLALSLAAPSDPKESDELTRIVAGMESAYGKGKYCASGKDKCLDVQDITRIMAESR
                     NPEELLDAWRGWHTISPPIRKDFSRYVELANKGARELGFADTGAMWRSKYDITPDQFA
                     GELDRLWEQVRPLYVSLHAYVRNKLREKYGDSIVPARGPIPAHLLGNIWAQSWENVYP
                     LVAPPDADPGYDLTQILKARNVDDLEMVRYGERFFTSLGFAPLPKSFWERSLFRKPRD
                     RDVVCHASAWDIDNVDDLRIKMCIEITGEDFATIHHELGHNFYQRAYNKQPMLFRDSA
                     NDGFHEAVGDTIALSVTPEYLVKLGLLKQVPDTSKDIGLLLTRALDKLAFLPFGLLID
                     QWRWKVFSGEVPPERYNQSWWELRQKYQGVSAPVARSEADFDPGAKYHVPANVPYTRY
                     FLADILQFQFHRALAQAAGCKEPLHRCSIYGNEEAGRRLISMLEMGASRPWQDALQAL
                     TGQREMDATAIRDYFAPLQKWLDQQNKGNPVGW"
     gene            10861..11214
                     /locus_tag="VLE22_00125"
     CDS             10861..11214
                     /locus_tag="VLE22_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12827.1"
                     /translation="MRVFDHVGVPTEEKRPGEMYVKDTKVWVTNPIEHPHKIEYLRFE
                     ADTPVTGPVRDLPHTAYRVDNLETEIAGEQVILGPFYSTPTMRVVFIEKDGAILEFME
                     NAEDGHWFRPEPEAK"
     gene            11211..11873
                     /locus_tag="VLE22_00130"
     CDS             11211..11873
                     /locus_tag="VLE22_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSB12828.1"
                     /translation="MRRLLSVVALAAAWTCLAAQVSSSDPAFAPVSDDPRLPRVLLIG
                     DSISIGYTLPVRKMLEGKANVHRIPENGGPTINGVQKIDAWLGDGEWDAIHFNWGLHD
                     IKLDANGVHQVPADSYERNLRELVKRMKSTGARLIWASTTPVPEGRLSPPRHPGDEVA
                     FNAIALRVMKENGVVVDDLYSFALPRLADIQQPANVHFTAEGSEALAKQVAASILRAL
                     ER"
     gene            complement(11881..12981)
                     /locus_tag="VLE22_00135"
     CDS             complement(11881..12981)
                     /locus_tag="VLE22_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12829.1"
                     /translation="MTVGFFSPLPPARTGVADYSAALLGALRHRGGVKVGDGDADVCL
                     YHLGNNRLHSEIYRCALARPGVAVLHDAVLHHLLLGMSGAEEYIEEFVFNYGEWHRQL
                     AAELWQNRARSGADGRYFRYAMLRRIAEASKAVVVHNPGAAAIVRAHAPSACVAEIPH
                     LFHPPSLPFGAETARLRGRLGLQPRTFLFGIFGYLRESKRLPVVLRAFEAIRSAGVDA
                     ALLIAGEFVSTDLARALERVLYRPDVLRVGHLPERDFWLHAAAVDACINLRCPAAGET
                     SGIAVRLMGIGKPVILSAGEENSRFPEGSCIRVAIGPGERLELAQHMTWLVQSPVAAR
                     EMGRMAASHIHERHSLDGTADLYWEVLSSCRG"
     gene            complement(12978..13637)
                     /locus_tag="VLE22_00140"
     CDS             complement(12978..13637)
                     /locus_tag="VLE22_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HSB12830.1"
                     /translation="MLQDRLNEVEDRIQRAAAKAGRRRDEITLVAITKVFPASVILEA
                     WALGLRDFGENYVQEFESKLPEVATLDGARFHFVGHLQSNKAKRAAELFHVIETVDAP
                     KVARRIDEAGRPLDVMIEVKLSGEASKSGADPAALPELVEVLRGCRNLRVTGLMTMPP
                     WSDDPEVSRPYFQRLRHLAEEYRLPHLSMGMSHDFEAAIEEGATHIRVGTALFGSRKR
                     A"
     gene            13664..15181
                     /locus_tag="VLE22_00145"
     CDS             13664..15181
                     /locus_tag="VLE22_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12831.1"
                     /translation="MRPPAGGDCNARSGETRTVIERFLNSSRRPILLEPGEKQFLLDD
                     DHYLLDVRNGGLIIQVWDESRNLVRRVTGVHSERPGKLELVIEKFGKRPGRLILADLS
                     RPAAATAERHGKRLAFREEFRRFLAREFPDWKATEVSTEPNLEYSLSPAYPRALLKRG
                     QSGMAAVSCPRDAGDATGALSFGLIWLDYLRRRERRLTVEGLTLFLPQGLEHTTCLRL
                     RWLDPTLARYLVFVYSPEGFTARVDLADYGNLDTKLEPCSSSPAAADGGVAPLVERLR
                     AIHKVECVECADGASLRVRGLQFARIDNGKLLFGIERMREAGAGSAGEIERLVRELSR
                     LRSAEATDRENPLYRRQPERWLESRVRAHIEQVDASLHPAPVYGQVPALAGGERGVID
                     LLATDIGGRLAVLELKASEDIHLPLQALDYWMRVRWHLERDEFRTMGYFPGVTLSKRP
                     PRLLLVAPSLDFHPTTEIILRYFSPEVPVERIGLAVEWRKTLRVAFRIEGAGCPI"
     gene            15191..16087
                     /locus_tag="VLE22_00150"
     CDS             15191..16087
                     /locus_tag="VLE22_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12832.1"
                     /translation="MASHILQQIRKALNNLNPNDVRKLAERQLTIGLAASSSEALAAM
                     EDYLVPPSVSHRRRQELVSVIHRIGNPGAPSRFDIEIIEERLPAAPGCFQFSMRDPER
                     VIREILLEREDLALPLARLLPPFRKRVTQRIIRDVARENAGFSLLTALPDILPSVLSL
                     PWAAGEFASDTTVLTVNQVRMAFLLAAASDRKVGYLEQKAQIASIIAGAFGWRALARQ
                     LIAKIPLGGGLIPKAAVAYAGTYVVGTGLERFYGRGYALTRAERKQAYQKALEDGKEL
                     SRLLLERARSATKRDRYASEKG"
     gene            16116..16916
                     /locus_tag="VLE22_00155"
     CDS             16116..16916
                     /locus_tag="VLE22_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12833.1"
                     /translation="MMLRMTALLAMTLSLVLADRLTLRDGREVEGTYLGGDARQVRMA
                     VGDKIESYRVSEITSLQFGGATAAAPVTSGSGATTTLAAETKPADTKPAAAAPASGKE
                     IPAGTEVVIRMIDDVDSEVSRVGQTYKASLDEPVIVNGNTLIPRNVDVTVKLIEDQQS
                     GKLTGRTELTLALVDVTVDGKQVPVVTQEVTQASESRTGRTAKVVGGTAALGAIIGAI
                     AGGGKGAAVGATTGAGAGAAVQVLTKGQRVRIPSETRLTFTLKEPVRL"
     gene            16978..17697
                     /locus_tag="VLE22_00160"
     CDS             16978..17697
                     /locus_tag="VLE22_00160"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HSB12834.1"
                     /translation="MITGASRGIGRGIAIELARTHQVLATYKGRRDAAESLRAETGAE
                     IFQCDVGSRDDRQALLEFARERFGRLDLLVNNAGMAPRERRDILEATEESFDELIATN
                     LKGPYFLTQQAARWMAEQGRGRIVFVTSISSYTASVNRGEYCVSKAGLSMAVALYAQR
                     LAAAGVQVFEIRPGIIRTDMIAAVEQVYEEKIAGGLLPQRRMGESTDIARAVRAIADG
                     LLDYSTGQVLNVDGGFHLRSL"
     gene            17759..19042
                     /locus_tag="VLE22_00165"
     CDS             17759..19042
                     /locus_tag="VLE22_00165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB12835.1"
                     /translation="MKKGFYGWWIVAACFLTFGVSTGFPYYNIAFFFDYFRDDHSWPI
                     SFITMGAPIAVLLTIWAGPVIVPRFSPRLLILVGTAMTFLAFQWFARLSGSRWEYFGA
                     WCLYMIGYFLSGPIPHQVIISNWFKEKRGRAMGITYVGVAVIGSIGNKLGPYLASQMP
                     YTDALKYMGFLLLLAWPVALFVLKDKPQDVGQFPDGKATAEEVAKPAVLEPPKSFGFL
                     TRKSAFWLLLVGSAASIGSIAAVNFHMKFVFEAQGFVEQAARDQIWGTASFWVLWSSI
                     AGRLLAGYLADKLPRKYVMLATYIVVALAIPTLFLVKPGHTGFLYVFALVFGFAMGAD
                     YMLIPLMAADQFGLSTLARAMSAILPTDTIAQFWFPNFVARLRDLVGGDYHIAMMAVF
                     GTAFLGAIAIGMLPRHGPKDEALHVQDARGTAARR"
     gene            18993..20429
                     /locus_tag="VLE22_00170"
     CDS             18993..20429
                     /locus_tag="VLE22_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12836.1"
                     /translation="MRHFTYKTLAELRRDAEVHGARHIVFEEEPSRVREILARKVKVG
                     PLIIGNSITIHPMEGCDGTPQGHPDELTWRRYERFAAGGAKLIWFEATAVREDGRANT
                     RQLWLHKGTADEFARLLETIRRIHRERCGTADDLIEPLQLTHSGRYSVPKRIIACHNP
                     AVDRKTGVPADYPVISDDELERLEDDYVETARLAVAAGFGAVDIKATHGYLLNELLSA
                     KLREGRYGGPLENRTRFIRNVIGKIRAALGNRLVLCMRLGCYDGVPYTTGPGNTGVPL
                     EHPIPYLYGWGVDPNDPFREDLNEVKQAIGWFKEWGIELLNVSMGSPYYNPHVGRPFE
                     KPDEGNYEPPEHPLLGVDRHFRIAGELQRAIPDLPMVGTGYSWLQQYAIHAGARNIAD
                     GNIRLFGLGRCAIAYPDFARDALEKGELDEHRVCKTVTFCTYLMRQKNHPLGQFPTGC
                     VPFDKDVYGPIMKEAREARRAAESKPKH"
     gene            20438..21988
                     /locus_tag="VLE22_00175"
     CDS             20438..21988
                     /locus_tag="VLE22_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB12837.1"
                     /translation="MRVILSVLLSAAGALSLSAAQNFDLVVYGGTAGGVITAVSAARE
                     GASVALLEPGRHVGGMVTGGLSRTDHGRKETVGGYSLEFYKRLGKKYGTEIEWYPEPH
                     VAEQVLLEMMKEAGVKVFYGRRLREKTGVRKKGRQVVEIVMENGAAFQAKVFADCTYE
                     GDLMAQAGVSYTWGREGSSQYGESLAGVRPKDRGHQFDFPISAYDAKGRLLPEIGTQP
                     RGELGAADKKVQAYNYRLIMSKEPSNRRAIEKPAGYDPWRYELLVRWLQGFEKHNGRA
                     PRINEVLLIGTCQGNKADFNNRGAVSTDYIGKSWDYPEASYKRRAEIWREHADYTKGL
                     LYFYVTDPRIPKSLQDDIRQWGYAKDEFTDNDNWPYQLYVREARRMTGGFVMTQKDIQ
                     TELTKPDAIGMGSYNSDSHNVQRFVQADRTVQNEGNMEVSVTPYQIPYRILLPKRTEA
                     ENLLVAVCFSASHVTYSTLRMEPVYMIIGHAAGVAAKMAIDKKVAVQEIDTKALTARL
                     RAQGAAME"
     gene            complement(22047..23654)
                     /locus_tag="VLE22_00180"
     CDS             complement(22047..23654)
                     /locus_tag="VLE22_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12838.1"
                     /translation="MSDPILAILWAQWRTLLNFRRGHGMGSFVLPALAILAWYGTWAM
                     LAVTIGFFVSQPGVRRMLPLVLPNGLLAVFVYWQLTPLLTASMGASIDLRKLLVFPIP
                     ARRLFEVEVLLRLSTCIEMMLLVSGLAAGLLVNPALPHRNGTLAFLPFIVFNLLVSAG
                     LRNQMERWMARRRVRELIVLFVLLAAAVPQLLFVTGVPGPLRWFVSAQAQMWWPWSAT
                     ARLASGEGTAALWLVLLGWTIAALFFGRWQFARSLRFDAAAAESRGRTSGESRPWLDR
                     LYRVPGMLFSDPLAVIIEKELRSLLRTPRFRLVFLMGFTFGVIVFLPIILRRGGGPLP
                     PGDHLAMVSGYALLLLGDVAFWNVFGFDRSAAQLYFLLPMPISRVFAGKNIAALFFVF
                     LEITAIICVWAAFRMPLTAVKAAEAYAVALTFSLYLLGAGNVSSVYYPRAINPERSTG
                     AASAGRLRAMLLLIYPAAAIPILLAYGARYAFESDTAFWSVIAIAAALGAAFYWVALD
                     SALERIERNKDKILRTLSEGEGPVRLS"
     gene            complement(23659..24417)
                     /locus_tag="VLE22_00185"
     CDS             complement(23659..24417)
                     /locus_tag="VLE22_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB12839.1"
                     /translation="MEDASPAIRVADLRKIYGDKAAVDGLTLEVPRGCFFGFLGPNGA
                     GKTTTIRMLIGLTPPSSGSIELLGLPMPEQALAIKRKIGLVPDESLLFDRLTGAEFLE
                     FVGRMYGLGRPVAKERARELLNLFELDGNPRQLIAEYSKGMRKRIAMAASIIHRPELF
                     LMDEPFEGVDAVGARMMKDLLLDQVRQGATIFLTSHVLEVVERLCDRVAIIHEGRLVM
                     ESAIGELRLGADTLEDVFVRTVGGERAIEKLDWL"
     gene            24505..25362
                     /locus_tag="VLE22_00190"
     CDS             24505..25362
                     /locus_tag="VLE22_00190"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSB12840.1"
                     /translation="MLTVRPTLARDKLLHRLDLLPPFSPILNRALASLSQENVCFAEV
                     ADLLEKDTVLAGNVLRVVNSALYGLQREVNSVRHAISLAGIHRVRNTVLALSVTRMWR
                     HPAEAHGWNTVAFSLHSAATACFSDLLAQLVPVEYPEGAFTAGLFHDLGKLLVANELP
                     RVYEVLRTMDGREESAILECELELAGIHHSALAAEALERWKLPPAIREAVRLHHAPGD
                     SSIGPLSLGQVVCTADRCVRALGLAVAPSAPPGIPAAQILDQSGAGAHAERILDEFRQ
                     ELDVLREFF"
     gene            complement(25377..26717)
                     /locus_tag="VLE22_00195"
     CDS             complement(25377..26717)
                     /locus_tag="VLE22_00195"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB12841.1"
                     /translation="MNRRTFLNTMPLGAATPLFVPASALGQANRPAATERVTMGVIGT
                     GNQGTGDMRGFLRDPRVQVVAVCDLNRMSAGYWDGDIAGREPARSYVEWHYGREKRSG
                     IYKGCDMYEDFRDLIARKDIDAVLIAVPDHWHSIPVILAARAGKDIYGEKPLSLTIAE
                     GRAMSNAVRDNKRVFQTGSQQRSDSRFRKACELVRNGRIGKLKTVTCGLPGGTPDLSR
                     DAHRRQPEPVPDGFNYQMWQGPAPEAPYCPARCHVNFRWILDYSGGQITDWGGHHPDI
                     AQWGMGTELTGPIEIRNAKGQWSDDGLYNTAVDFTFEAIYENGVRMVISNKTRGGVTF
                     EGTEGSVWVNRGSIESKPDNLIYSDITDHELHLYKSDDHFRNFIDCVISRKEPVAPIE
                     QAHRTITIAHLGNIALKLGRNLKWDAAAERVVNDDEANRMLSRPMRAPWGLPPA"
     gene            complement(26773..27897)
                     /locus_tag="VLE22_00200"
     CDS             complement(26773..27897)
                     /locus_tag="VLE22_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12842.1"
                     /translation="MSESLKGCVYPSEHSQFRDRVTGATIHQMTRHLSISHPTYFLHS
                     SFTPDGQAIIFTSYRTGQAQLLEAGFPLGGIRQLTDGEPIHPFSPLIHPDGERILFVR
                     GGGVWAVERTSLREHRVHEFPGAQLGECSLDAAGEWITAAIRQDSGFGIAVGRCNGEG
                     WHVIAFPRTVIHPQFHPLEPEWIEFAADPAPRMHRVRRSGSGLECLYEHGNDEFIVHE
                     TFLGRTGDLIYTVWPKLLCRMDWRTRERNVIAEFNAWHISADRAGARVLCDTNHPDEG
                     LFLVDVASGQRRRICLSESSNQGSQWTKSRYALAEDFQAARSARRGALSWMETATDTV
                     YGPQWTHPHPSFSADERYAAFASDRDGHTQVYVADLSRSQ"
     gene            28040..28585
                     /locus_tag="VLE22_00205"
     CDS             28040..28585
                     /locus_tag="VLE22_00205"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HSB12843.1"
                     /translation="MITRFSLRIPHFLSLMFLAACLLLAGCSEASNSARKRLYHMGER
                     VEAGALVYTVLEAEWQPQLGEGANARIPQHQFLMLRVSITNSSIREISIPQLRLVGSR
                     RDSHAELTDGDRVPNWLGMLRSLKPADTIEGRILFDVPREDFQLEVSDDALDPEQRNV
                     ALIEVPLRFESGNADLPQRTR"
     gene            28599..29435
                     /locus_tag="VLE22_00210"
     CDS             28599..29435
                     /locus_tag="VLE22_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12844.1"
                     /translation="MTQTWLGFVLLLTLQQAPVGTFGITSPTLHQFEDGPAIPAGHRF
                     IPGETVFFSCQVQGYRATPEQKVRLSYKVDAVDADGVLLVATDTGKIDTELAPQDKEW
                     MPKIRRAILIPPHAPPGQYRIVTTVKDEVGGQEAKTELSFQVHGRRVEPSDTLVVRNF
                     RFLRSEQDDNPLPIAFYRPGNTLWARFEMTGFKIGEKNRVHVEYGIAILSPDGKQLFA
                     QEQAAVEEDSPFYPKRYVPGQLSLNIQPKTPPGEYTLVVSVRDQVGAQTIETRQPFRI
                     EE"
     gene            complement(29477..>31611)
                     /locus_tag="VLE22_00215"
     CDS             complement(29477..>31611)
                     /locus_tag="VLE22_00215"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HSB12845.1"
                     /translation="QVRSEAAAVIGQRAEALAALIEVDPAEALKLAFAEDLLGTLSRA
                     FPGSAAVLEAHGSWKGVLEYQITDDPTMTRHQSIQRLRYGQESLEIHFAGSEPKGLKG
                     GDIVSVRGIRAGNRVAAQDGSILGTDAVAAACSTKGAQNIVAILVNLASYTLSSGVDQ
                     ELMKGILWGNDFTTKDATPNRSVDNFWYESSDHQTWAPASGGNVVGPYLLSQDYNKNS
                     SGQTYCDYNGLKAEALQQADAEVYFPSYSRVMLIFPPNGACGWAGLGDLGCWNSGTQD
                     GGFNASFVWERSDQTANRLWGVELATHELGHNLSMHHASSREFTDAGGARVPLGALGD
                     AGTLTEYGDLFSTMGSWNLGYYSAHHAEQQLGWLALGANYQVVETSGLYTILPYESFG
                     GLKALKVRRGTGNDAWLWIESRRAIGNYDSAIGSQVFGGALIHYEDSTTGTKTHLADF
                     TPSTTSFNDPALLAGQTWADPYSNVSVRVVSVSDSGMTVEVNYGAVPCTSAAPTVTIS
                     PSNPTVQPGGSANYTVSVKNNDSTGCPAGSFNMSSTLPSGWGSSFTLNAVTVNPGETG
                     ATTMTKTAPSGTGAGTYQVNAAATKGSYSGEGTANATVPAPPPIVAVKLSFLSTGPYQ
                     TGSIVKLKAAVTNDGSPAAGAAVTFKIVKSNGSSTKSKRITTNSAGEAFWNYTIGKRD
                     PRGNWQASATSTYNAQSVSSAPLTFTVQ"
BASE COUNT         6051 a         9719 c        10010 g         5831 t
ORIGIN      
        1 ccttgatgtc gatcggtccc gacccagtat actcacgggt ggatggacta cgacaccttc
       61 attcagaagt tgcgggccta ccaggacagc cgcatcctgc tgaccgcgat cgaactgcat
      121 ctgttcaacg cggtcggcgc cggcgccccg gcggaagaga tcgcggagcg tctcgacacc
      181 gatccgcgcg ccaccgaaat gctgctgaat gcgctggtcg ctgtcgagat gctgaaaaaa
      241 cgcgacggcc tgtttaccaa cactccgctg gcggctcaat acctgactgc tggttcaccg
      301 gacaacgagc gcgacgcctg gatgcacagc gtgaaccagt gggcgcggtg gtcccggctg
      361 acgtcgtgca tccgcaccgg aaaggcgccg gtcttgcccg agatcggagc gcgtggcgaa
      421 tcatggatcc gggcgttcat cgccgccatg caccgcaacg ccgcgggaag ggcgccccaa
      481 ctggtcgaga ccgtgggcgc gagcaccgtc gcgcggatgc tggacatcgg cggaggctcg
      541 ggcgcgtatt cgatcgcatt tgccctcgca aacccgaacc tccacgccga agtcttcgac
      601 ctgcctgagg tgcttccaat cgcgcagggg catatcgacg ccgccggcct cactggccgt
      661 gtggtcgccc gcgccggcga cctgcgccgg gatggcttcg gtgatgggta cgacctgatc
      721 ctcctctcgg cgatctgcca catgttcgga gaggacggga atcgcgatct gcttcggcgc
      781 gcgtaccggg ccttggcgcc ggcaggccgc gtggtaatcc aggatttcgt gcttgaggaa
      841 gacaagacca cgccccgcat gggggcgatt ttcgcactga acatgctggt cagtacccac
      901 gaaggcaaca gctatagcta tcaggaatac gcaggctggc tgaacgaagc ggggttccag
      961 gacgtttcca ggcactggcc gggcgagcct accggcctca tcgtcggcgt tcgaggctga
     1021 ccgccaggtg tatactcggt gctttccaaa caggataagt gacatgaaga tcaccagaag
     1081 acacgcgctg cttggggctg ctgcgcccct gatcgtgaaa cgggagaccg cgttcgcgtc
     1141 gcaagcgaac tccgccgtcg ccttcggaat catcgggacc ggaggacgtg gacggtacgt
     1201 cggcgggttc atggccaagg actcgagcgc gaaactggtg gccatttgcg acaagtaccc
     1261 ggaccggatc gatctcggca aaaccgaaat ccccggcgcc gaccaggccc ccgctttccg
     1321 tgactatcag gaactgctcg ctcgcccgga tatcgatgcg gtactgatcg ctactccagt
     1381 ctttctgcac cccgagcatt tcgaagccgc cgtcaaagcc ggcaagcacg tctactgcga
     1441 gaagccagcg ggcgccggcg tcgccggatg caagcggctg ctcaaagccg gcgaccgagc
     1501 cgacacatcg aagacgatcc agttcggatt ccagcagcgc ttcagcccgg agtacctgac
     1561 ggcgatgagc ctgctccgat ccggccgcat cggagacatg aggttaatga tgagttaccg
     1621 gatgctcggc gggctgccgc ctgccgcgtt caccaacccg tacactggcc cggacgagaa
     1681 gatcaggaac tggggggctt gggtcgccta ctccggcggg ccgatcgtcg agcaggactg
     1741 ccacggcgtc gacacgctga actggttcgc cggcgacgtc catccgacgc gcgcgttcgg
     1801 aacggctggc cagcgctacc cggtgcccta cggcgactgg aagaccgact atcacgatat
     1861 tgcgttctac tacccgaacc agctcgaggg ctggctcatc agcatcaagc acaccgccgg
     1921 ctatcgcgac gtgaaggagc agcttttcgg atcgaagggc ctgcttgaaa cgtcccgcag
     1981 ttactacaaa ctgcacggtc cggaaggcaa ctggcgctac ccgaacgcgg acgacctgcg
     2041 cgaccgcagc ctgatcgaga aacgagactc ggcccgtgag atcacgatcg acgccgtgga
     2101 ggcgttcttc aagagcatcg tcgagaagaa gccctatcac atgtggaaga cctcggccga
     2161 aagcacgctg acggcgctcc tcggccagat ggcggtcgag aagcggcgcg aggtgacttg
     2221 ggacgaactg ctccgctcgg cgtagccgcc gtcattttcc agcggcacgc gagatgacgc
     2281 gccgggccgt cttcccggcc actcgaaggt cgaagccgtc cttcaattcg ctgcccaggc
     2341 ggatctcttc gttgcccgtg tcgggatcga gaacgcggta gagcgtgttc tcgtccaccg
     2401 tgtaccactc tggccactgg ttgaggcgag cgtaatcctt cttcagattg agaacgcggc
     2461 ggtagcgggc gtaatctagg cagaccgagc cggaccaggt gcccgccgag tgaacgctga
     2521 tatagaggtc ctccccgctg cgaaccgcgc cgagttcaac gcccggtgcc caatccttga
     2581 gccgtgcacc ctgggtcttc atcatggcgt agagcagtaa ggtccggttc cagttgccgt
     2641 caccgtacca gcgttcgatg acgccgtccg gctgttggta aggatacatc ttcggaatcg
     2701 agtgatcgat ccaggcgagg gcttcgggga cgggttcgtg ggcgacgagg tagatggcgc
     2761 cctcgatgga gtcggccaga tcgtcgaccg tgttaacgcg cttcgtccag tcgtagtcgc
     2821 ggtacttcgc gagattcttc aaaacgcgaa gaaccgcctc gcggtacttc tgctcgccgg
     2881 tggtctggta gtaagcgtac atcgacgcgt agacgtagcc ccagcaatcc gttagcgccg
     2941 cgtcgaccgg ctgaaggtcc gccgtacgga tctggcgata gatgaatccg tccgggttgg
     3001 ccgattcgag cacacggtcg aaggtcttgc gcagcacagg ccgatagctt ttcgcccgag
     3061 acccgccaag ttcggtttcg agcgcttgga gcaggacgag gccgacgatg gcctcgttgc
     3121 cgtggtcccc gaggcccacg gtttcagtgg cggtcttctt gtcgaaatcc cagccatagg
     3181 ccgggaggta gttactgccg ggaaggatct cttcgatgta ggcgtctccg atctgctcgg
     3241 cccacttgag gaagcgcggg tcgccggtca tggggatcag gcgcgcaagg gcttgaagca
     3301 cgtcgccgtt gagttcggcg cctgtgtcgg gaaggttgcc aaatcgggtc ttcaccggag
     3361 cgcgcgccat gaagtcctcc atcatgtcga ccatccggta gaaccacggg gtccggccga
     3421 ggagttcggt aaccggcacc atcccgtcct tgcagtactc gccggccccg aagaagctgg
     3481 gcggcccgag ttcgcgcgtt ttgaagtcta ggttgcccgg gatggcgcca cctccgacgt
     3541 tagtaaagcg cacctcgttg cgaagcatct ccatcatccg gcctcggtaa agcgctggct
     3601 cggtgaagaa agcggtcgtg atgagaaacg ggtagttgtc ggcgccggag ttgtgcgggg
     3661 tgtagacaga gcgatcgtta ggcttgccgc gaatcaggcc gggcagcccg tccgggagca
     3721 gcaaggtttg ggaatccgca tgtgccaacc agccgtgcat catcttcctg gtgcggcgaa
     3781 agatgtcgtc cataatcgca ccgttggcgc gggctgtttt ccaaatgtcg tctggaggga
     3841 cgccaagggc attcaccgcc gccgcggcgg ccaggagcag gctaaatcgt ttcatctcag
     3901 gattccccgc tgacaattct cgtttatccg cgggcagaaa aaaatcttcc gccggagagg
     3961 gggaaacggt ccgccggcac gactacggga aacagagggg ccgcaaggga aatgcgagcg
     4021 gcctccacga aacctttctc tggaggataa caatgacttg cagccggacg ccgattctgg
     4081 gcgtcgcttc gatacttctg ttcgcttcgt tggcgctcgg taaccctggc aaaccgaatt
     4141 tcggaccgtc ggtttacgga gatgggaagg tgtggggcac caagggtgcg gccaccctct
     4201 cggcgccgaa cgacaacaac atgcagtcgt acgacaagct gtttgtgttc gtcaatggcg
     4261 cgatggggca gatcccggtc gcggaggccg ctccgggcaa ccccatgtac aacggtggcc
     4321 gctggcacac gcacacggtc gtgtggacgc aggcgggtct cgatttccat ggtggaactc
     4381 tccccgtgct cacctcgtac gatgagattc agtttcatta cgggctgggc cacctgatga
     4441 tcacacccgg ctcgccagcc ggagggcccc ctccttactt tgagtgtccg ctgctgccgg
     4501 tgaagtagca tacgggggtc gcaacggggc aggcgcgcag cctgccccgc atgttggtcg
     4561 acccgcttgg cagcgcacgg acccgctgga atcagattcg tgttttcgtg tatcctcata
     4621 ggaacctcaa tttgtcgcgc cgtgagattc cttcttctct tggcactcgt gccaccggcg
     4681 gccgccgcac cggcctcgtt cttcggcggg ttcgtgggtg tgtccacgct ctcggcggac
     4741 ggcgcatttt cgacccgtgg aaacacgagc gcgcactccc aatacaagcc agaaaacggg
     4801 ccgactgcca tggtgttcgc cggacggcac ctgaacgact tcttcgcagt ccagctcagt
     4861 tacgggtgga accggaacga ggtgctactg gcggccacgg aagccgggac gacggctaac
     4921 gcctacgaac agggccggag gaaccggcag cacaccgtca tcggggaggg catggtattc
     4981 ttccgcaagc ggcaaagccg cgtgcggccc tatctgaccg ccggagcagg cgtgcatcag
     5041 ctcaccagca aggccaacgg ccgcctcgtc ctgatcggaa accccgtcct tccgccggcg
     5101 gagttcagca agactggccc ggccttccgg gtagcggtcg ggatcgacgt attcctccga
     5161 ggcggtttcg cctggcgata cagcttcagc gagacgatcc agggcaaccc tatgagcaaa
     5221 cagttgtcgc cccagggcgc ccggaacctc aagaattttc agaatctctt cggtttcgcc
     5281 tggtacttct gatcgccggg cgcggttacc cccgccgtca acggttctga aaacgaacat
     5341 agggacagcc aggccaccag atcggtgcgt tttcgatgtg ttctgctaac agccgcgcat
     5401 ggcagacctt gcagaagaac gccgattcgg tccgggctcc ggcggggaca atctgtacca
     5461 actcttcgaa cacgcacagc ttccggccgt tctgtttgga accggtcagg ctgagcagcg
     5521 gaccactctc ctgctgacgc tggaccaagg cggtgtaggt cctgctcttg agccacgcgg
     5581 gcgccttccg ttccggcccg tacggcgctt cggcgacgct ggccagacgt ttgaaatcga
     5641 gatcatcagc cattctgaac ctcgctttgc cagcggcggc ggaagtgttc tcgcgctcta
     5701 gccagggcgc gctcgacgcc tttcacggac ttacccgtct gccgggccat ctcttccgcg
     5761 ctctgccgtt cccagtaccg ccagaccagg aggatgcggt cgttttccgg cagagtgccc
     5821 agcattttcc agaccagaga ttgttcctct tctcgctcga tcgaagcctc cagactctcc
     5881 tccgcggcag gttccgcgtc ctcttcgctc cactgggcga gccggagccg ctcgcgaaag
     5941 tgatcctgga ccttgtgacg cgcgatgccc agaagccagc ttttcaagcc gctatcgccg
     6001 cggtagcccg gcagggcttg ccacgcatcc aggaagactt gctggaagca gtcatccgcg
     6061 acgtcggcgt ggggtagcag ccggctgctc agatacgagt agacagaatc ggagtaccgc
     6121 gaaacgaatt cctccgtcgc ctttcggtcc ttgcggagca cggcgtggac cagttccgtc
     6181 tcgctggccg cttcttcccc ttgagaaacg gcagcatcgc cgcgagcagc cggggttgtg
     6241 gcagaggatc cgctcacatc aggatgtcgg ttgccggggg caaatccccc ttccgggtcc
     6301 atggctccgc gccctccatc atgacgcatt ggcggcaccc acgaagggtg gccgttcgat
     6361 tccctgggag tttcggtatg atcgatacac aagggaacat cgatgcaata cagactgact
     6421 gctgttgctg ttctgctggt tgtggcggcc gggctttccg cgccgaatgc gctgacgcag
     6481 gcgccggccg gttttgtctc gctgttcaac gggcgcgatc tgagcggatg gacggtgccg
     6541 gagggtgaca acgggcactg gaaagtggtt gacgacgtga tcgattacga cgcgaggagc
     6601 gaatccccga aggacaagaa cctctggtct gagaaagaat ataaagactt cattctgcgc
     6661 gtagactggc gcatcaaggc gaccccctac cggaacaagg acgctcgtat cgtcatgccg
     6721 gatggttcag acaagctgga cgagaacgga aaggtaatcc agctcgacct gcccgactcc
     6781 gactccgggg tttttctgcg tggaacgggc aaggggcagg taaacatctg gtgctggccg
     6841 gtggggtcgg gcgagtttta cggctaccgg acggacccga agatgcccgc tgaggtgcac
     6901 gccgccgtga ctccgaagat gaacgcagac cggaacatcg gcgagtggaa cacgttcgag
     6961 atcacggtaa gagggaatgc agtgacggtt gtgctgaacg gcaagaaagt gatcgacgcc
     7021 gcgaagctac cgggcctgcc ggagaagggc cgcctcgcac tccagcatca cggcagcttc
     7081 aaggacggcc agtggcaagg tccgccgagc ttggtgcagt tccggaacat ctacatcaag
     7141 gaactctgac ggggttccgg cattgtttcg gcatacccgg tcctacttaa cgggccccac
     7201 gtactcggtg ctgatcacca catcatccaa ccagtaggta cggtcgtgct gactattgct
     7261 gctgtaattg ccgaacatcg ccatattcag tttgaggctt tcgacatccc gccagcgcat
     7321 gccgtccaca cgcagaattt ccttaccgtc gatccaaagc acctgctcgc cgtccttctt
     7381 gccgggttgg ttacatttca ccagcatctc gaaacagtac cagcggcccc gctcgatcca
     7441 gaaaggttcc ttcgggtaga agtagttccc ccagtaacgg ccatctggtg actgcttcat
     7501 gtccggccag taggtgtaga aaacgatctg tccaggaggc ggcatggacg ccacgcccct
     7561 cggctcgaca tttacgatga agcggtccgt cccatcgggt ttgcgcccgg caccgcccag
     7621 caccgaacga gggctatccg gacggcaggc ccagaactgg caccagtgca tcccccgccc
     7681 ttcaaaattc tccgcaaact tcgcatacca gcggatatac acccggtcgt aaccgggcag
     7741 gaaccaggcc cggatataac tgtcagactc ccggagaacg cgtccctcaa cggtcttgcc
     7801 ttccccctga aaagccgggg ctgagatccg atagctcttt gtgcccgcgt ggacatactc
     7861 cttccgggtt tcaaaccctc cacgaacagg gtcctcgctg tacttctccc agcttccccc
     7921 gagttcgcct gactcgaagt tctcgctgaa gaccacttgg ccgagcgcgg ccgtgcagaa
     7981 cagtccagcg gcgacactcg cacgggcaaa aaaactgatt gttgtcacag gcatgtgggg
     8041 cgtttcttta ggccaatttc catcgataac gggcttctgg gtcagtatat ttcaaccgga
     8101 cggatcagcc gagatgtcgg cttctggaag gggcgatggg tctctgatgg ggcttcgggt
     8161 tggaggcgcg ggcgggaatc gaacccgcga ataaaggttt tgcagacctc tgccttacca
     8221 cttggctacc gcgccgcttt gatgtcagga taacgcaaaa cggcccaggc ctcaatgagg
     8281 gtacgggaga ccgctccgcc gtgacgcatc tcttccccgg atgcgtcacg cgactgccgg
     8341 ctgcaaattc tacacaggga cgcatcctga gatttcgtgt ccctgtaggg aacacaatcc
     8401 aacctgttga ttctattgtc aaagaacggg cgagtggcgc atcttggagt aaaatgcgcc
     8461 actcgaaggg gcttcctcaa acggacaacg gaagatgcgg cgccacatcc acccacattg
     8521 ttaacacggg gaaggtggaa ccgacgctcc ggtagaatcg gatattgctg aaaataagac
     8581 tacaatcgag acgttgcggc cggtgactgc caaatgagac cgttccagcg ttacgtgggc
     8641 atccaattcc cgagttgggt cctgacggcc atggtcgtgt ggggtctaca ccactggacg
     8701 ggcctgcccc gggggtgggc gatcaccctc ctcctcgctt acgttgccaa ggacttcctc
     8761 ctatacccgc tgctgcgccg gtcctatgaa gaagaccggc gaaccggagc ggagccattg
     8821 atcggcagga ccgggatcgc cgtcgaggaa ctgaacccgg ccgggtacgt gcgggtccgc
     8881 ggggaactct ggagggcgga ggcggccggg ggcgcggtgc ccgcgggcgc ggcggtgacg
     8941 gtggtttcga ccgacggcat gaccctggtg gttgaggcac agagggcgca ttgaaaacac
     9001 cgtttcgtgc ccttaccagc cgacagggtt gcctttgttc tgttggtcga gccacttctg
     9061 aagcggggca aagtagtcgc ggatggcggt ggcatccatc tcgcgctggc cggtgagcgc
     9121 ctgaagcgcg tcctgccagg gacggctggc gcccatttcg agcatggaga tcaggcggcg
     9181 tcccgcttcc tcattcccat aaatcgagca gcggtgcagc ggctccttgc agccggccgc
     9241 ctgggccagc gcccggtgga actggaactg caagatgtcc gcgagaaagt agcgcgtata
     9301 agggacgttc gcaggcacat ggtatttggc tccagggtcg aagtccgctt cactgcgcgc
     9361 gacgggggct gacacacctt gatacttctg acggagttcc caccaggact ggttgtatcg
     9421 ttccggcggg acctctccgg agaagacctt ccagcgccac tggtcgatca gcagaccgaa
     9481 cggcaggaag gcgagcttgt cgagcgcacg ggtgagcagg aggccgatat ccttcgatgt
     9541 gtccggcacc tgcttcagca atccgagctt gacgagatac tcgggcgtga cggacagggc
     9601 gatcgtgtcg ccaacggcct catgaaagcc gtcgttggcg ctgtcccgga acagcatcgg
     9661 ctgtttgttg tatgcgcgct ggtagaagtt gtgcccgagc tcgtgatgga tggtagcgaa
     9721 atcctcgcct gtgatctcga tgcacatctt gatgcgcagg tcgtcgacat tgtcgatgtc
     9781 ccaggcgctc gcgtggcaga cgacgtcacg gtcgcgcggc ttgcggaata atgaccgttc
     9841 ccaaaacgat ttcggcagcg gcgcgaagcc aagcgacgtg aagaagcgct cgccgtagcg
     9901 aaccatctcg aggtcgtcga cgtttcgcgc cttgaggatc tgggtcaaat cgtagccggg
     9961 gtcggcgtcc ggcggcgcga ccaaaggata gacgttttcc caggactgcg cccaaatatt
    10021 gccgagcagg tgcgccggaa taggaccgcg ggctggcacg atgctgtcgc cgtacttctc
    10081 gcggagcttg ttgcgcacat aggcgtggag ggagacgtag agcggtctta cctgctccca
    10141 gagccggtcg agctcgccgg cgaactggtc cggcgttatg tcgtactttg agcgccacat
    10201 agcgccggtg tcggcaaatc caagctcgcg cgcgcctttg ttggccaact caacgtagcg
    10261 gctgaaatct tttcgaattg gcggtgagat cgtgtgccag ccgcgccatg cgtcgagcag
    10321 ttcctcggga ttgcggcttt cggccatgat gcgggtgatg tcctgaacgt cgaggcactt
    10381 gtcctttccg gacgcacagt acttgccctt cccgtaagcg ctctccattc cggcgacgat
    10441 gcgggtaagc tcgtcgcttt ccttggggtc ggagggcgcg gccagactca gcgccagcct
    10501 cagcagtttc attttgcggg cgagatccgg tggcagggag acgccgtcga agcgcgtgga
    10561 ctgcttcgcc agttcaacgg tggcggtgat catccgctgg ttagctttgg cggcgagaat
    10621 ctccgagtcc atcatgatgt aggttgcctg gacccaactc gcgtggcctg cctcttcgga
    10681 gagcgccatc agttgtgtct ccgcgtcgtc gaggaaagcc cttgcctcag ctacggtcgg
    10741 cggcccggcc gccggcaatg cggaggcgac cgtcaagaga agtacgaaaa tgacaaagac
    10801 cctggttttc atgtacgtgg tccggtcttt tcttattatg ctgtagagac ggagagagcc
    10861 atgagagtct tcgaccatgt cggtgttccc accgaagaga agcgccccgg cgagatgtac
    10921 gtcaaggaca cgaaggtctg ggtgaccaac ccgattgagc acccgcacaa gatcgagtat
    10981 ctgcggttcg aggccgacac cccggtcacg ggaccggtcc gcgatctgcc gcacacggcg
    11041 taccgcgtgg acaatctgga gactgagatc gccggggagc aagtgatcct cggcccgttc
    11101 tattccaccc cgacgatgcg ggtcgtgttc atcgagaaag acggcgccat cttggagttc
    11161 atggagaacg cggaagacgg ccactggttc cggcccgagc ctgaagcgaa gtgaggcggc
    11221 ttctcagtgt cgtcgcactc gccgcggcgt ggacgtgcct cgccgcacag gtctcttcct
    11281 ccgacccggc attcgccccc gtaagcgacg atccacggct cccgcgggtt ctcttgatcg
    11341 gcgattcgat ttcgatcggc tacacgctcc ccgtccgcaa gatgctcgaa ggcaaggcca
    11401 acgtgcaccg catcccggaa aatggaggcc cgacaatcaa cggggtccag aagatcgacg
    11461 cctggctcgg ggacggcgaa tgggatgcga tccacttcaa ctggggcctg cacgacatta
    11521 agctcgacgc aaacggcgtc caccaggtgc cagccgattc gtacgaacgc aacctgcgcg
    11581 aactggtcaa gcggatgaag tcgacgggag cgcggttgat ttgggccagc acgactccag
    11641 tgccggaggg aagactcagc ccaccgcgcc acccggggga cgaggtcgcc tttaatgcca
    11701 tcgccttgcg ggtgatgaag gagaacggag tcgtagtgga cgatctctac tcgttcgcgc
    11761 tgccgcggct ggcagacatc caacagccgg cgaacgttca cttcacggcc gagggttccg
    11821 aggcgctggc gaagcaggtg gcggcgagca tcctgcgcgc tctggagcga tagcgggccg
    11881 tcagccgcgg caggaactga gcacttccca gtacaggtct gcggtgccgt ccagcgagtg
    11941 ccgttcatgg atgtgggatg ccgccattcg tcccatctcg cgcgcggcca cgggtgattg
    12001 cacaagccaa gtcatgtgct gagcgagttc gagtctttct cccggaccaa ttgcgacacg
    12061 gatgcacgag ccttccggga aacgcgagtt ttcttcgccc gccgacagga tgaccggctt
    12121 gccgattccc atcagccgga cggcgatgcc ggatgtctct ccggccgcgg ggcacctgag
    12181 gttgatgcag gcatccaccg cagcggcatg gagccagaag tcgcgctccg gcaaatgccc
    12241 cacgcgcagg acgtccggcc ggtaaagtac ccgttcgagg gcgcgggcaa ggtctgtgga
    12301 tacgaactca ccggcgatca agagcgcggc gtcgaccccg gcggatcgga tcgcctcgaa
    12361 ggcgcggagc accacgggca ggcgtttgct ctcgcgcagg tagccgaaga ttccgaaaag
    12421 gaaagttcgc ggttgaaggc cgagtcggcc gcgcaaacgc gccgtttccg ctccgaatgg
    12481 aaggctgggc gggtgaaaca ggtgagggat ctcggcgacg caagcggacg gagcgtgcgc
    12541 ccgaacgatc gccgccgcac cggggttgtg aacgactacg gccttcgagg cctcggcgat
    12601 gcgcctgagc atggcatagc ggaagtacct gccatcggcg ccggagcgcg cacgattctg
    12661 ccagagttcg gcggcgagct gtcggtgcca ctccccgtag ttgaaaacga actcctcgat
    12721 gtactcctcg gcgcccgaca tgccgagcag gaggtggtgg agcacggcgt catgtagcac
    12781 ggcgacgccc ggccgcgcga gcgcacagcg gtagatctca ctatgcaggc ggttattgcc
    12841 gaggtggtag aggcatacat ccgcatcgcc gtcaccgacc ttcacaccgc cacgatggcg
    12901 aagagcgccg aggagagcgg cggagtaatc cgcgactcct gtccgggcag gaggtagagg
    12961 agagaaaaag ccgacggtca cgctcgcttt ctcgatccga acagcgctgt accgacccgg
    13021 atgtgggtgg cgccctcttc gatggccgcc tcgaagtcgt gcgacatgcc catcgagaga
    13081 tgcggcagcc ggtattcctc agcgaggtgg cgcagacgct ggaagtaggg gcgcgatact
    13141 tcggggtcgt ccgaccacgg gggcatggtc atcagtccgg taacgcgcag atttcgacag
    13201 ccgcgcagaa cctcgaccaa ctccggcagc gcggcaggat cggcgccgct tttcgaagcc
    13261 tctccggaga gcttgacctc gatcatcacg tcgagcggcc ggccggcctc gtcaatgcgc
    13321 cgggcgactt tcggcgcatc cacggtctcg atgacatgga acaattcggc ggcgcgcttc
    13381 gccttgtttg attgcaggtg accgacgaag tgaaaccgcg ctccgtcgag cgtggccacc
    13441 tccggaagtt tcgactcgaa ctcctggacg tagttctcgc cgaagtcgcg caggccgagc
    13501 gcccacgctt cgaggatgac ggacgcggga aaaaccttgg tgatggcgac cagcgtgatc
    13561 tcatcgcggc ggcggccggc cttcgcggca gctcgctgaa tgcgatcttc gacttcgttc
    13621 agcctgtcct ggagcatcgg aacaactctt ataataaggg ggaatgcgtc ctcccgcggg
    13681 aggcgactgc aacgcgcgtt ccggcgagac gcgcacagtg attgagcgtt tcctcaactc
    13741 cagccgccgg cccatcctgc tcgaacccgg agaaaaacag ttccttctcg acgacgacca
    13801 ctatctgctc gacgtccgca acggcggcct gatcattcag gtctgggacg agtctcgcaa
    13861 cctggtgcgc cgagtcaccg gggtccatag cgagcggccg ggcaagctcg aactcgtcat
    13921 cgagaagttc ggcaagcggc cgggccgcct gattctggcc gatttgtcac gtcctgcggc
    13981 tgcgacggcg gaacgccacg gaaaacggct ggcgtttcgg gaggagttcc ggcggttcct
    14041 cgcccgcgag tttcccgatt ggaaggccac cgaggtgagc accgagccaa acctcgagta
    14101 cagcctctca ccggcatatc cacgtgcgct ccttaagagg ggccagtcgg gtatggcggc
    14161 ggtgtcttgt cctcgagacg ctggagacgc taccggagcg cttagcttcg gcctcatctg
    14221 gctggactat ttgcggcgac gggagagaag gctcacggtc gagggtctca ctctgtttct
    14281 gccgcaggga ctggagcaca cgacatgtct ccgcctgcgc tggctcgatc ccaccctcgc
    14341 gcggtacttg gtctttgtgt attcgcccga aggcttcacg gctcgggtcg atctggccga
    14401 ctatggcaac ctcgacacaa agctcgagcc ttgcagcagc agtccggccg cggcagacgg
    14461 cggtgtggcg ccgcttgtcg agcggctgcg ggcgatccac aaagtggaat gcgtcgaatg
    14521 tgccgatggg gcgagcctgc gggtgcgcgg ccttcagttc gcgcggattg acaacggcaa
    14581 gctgttgttc ggcatcgaac ggatgaggga ggcaggcgcc ggaagtgcag gtgaaatcga
    14641 gcggcttgtt cgcgagctgt cccggctgcg ctccgcggag gcgacggacc gcgagaaccc
    14701 gctgtataga cgccagccgg agcgctggct ggaatcgcgc gtacgcgctc atatcgagca
    14761 agtggacgct tctctccatc cggcgcccgt ctacggccag gtacccgcct tagccggcgg
    14821 cgaacgcggc gtgatcgacc tgctcgcgac agatatcggc gggcggctgg cggtgctgga
    14881 actaaaggcg tccgaggata ttcacctgcc gttgcaagcg ctcgattact ggatgcgggt
    14941 gcgctggcat ctcgagcgcg acgagtttcg gacgatgggt tactttccgg gtgtgacgct
    15001 ctcgaagcgc ccgccgcggt tgttgctggt cgccccgtcg ctcgatttcc atcccacgac
    15061 cgaaatcatc ctgcgctatt tctcgccgga ggttccggta gagcgcatcg gcctggcggt
    15121 agaatggagg aagacccttc gagtcgcatt tcggatcgag ggcgccggct gtcccatctg
    15181 agcgagcggc atggcgagtc atattctcca acagatcaga aaggccctga acaacctcaa
    15241 cccgaatgac gtccgcaaac ttgcggagcg ccagcttacg atcggcttgg ccgcttcgag
    15301 cagcgaggcg ctggcggcaa tggaggatta cttggtgcct ccatccgtct ctcataggcg
    15361 gcgtcaggaa ctggtctcag tcatccaccg gatcggaaac cccggcgcac cgtcaaggtt
    15421 tgacatcgag atcattgagg aaaggcttcc ggccgcgccc gggtgcttcc agttttcgat
    15481 gcgtgacccg gagcgcgtga tccgtgagat cctgctcgaa cgcgaggacc tggcgctgcc
    15541 cctcgcccgc ctgctgccgc cgtttcgcaa gcgggtgaca cagcgcatta tccgcgacgt
    15601 ggcgcgggaa aacgccggct tttctttgct caccgccctg ccggatattc ttccgagcgt
    15661 cctcagcctc ccatgggccg ccggcgaatt cgcctcggac acaacggtgc tcaccgtgaa
    15721 ccaggtgcgc atggcctttc tgctcgctgc ggcgagcgac cggaaggtgg gctacctcga
    15781 gcagaaggcg cagattgcct cgattatcgc cggtgcgttc ggctggcgag cgctggcccg
    15841 tcaactgata gccaaaattc cactgggcgg tgggctgatt ccaaaggcgg cggtcgccta
    15901 tgccggcact tatgtcgtag ggacaggctt agagcgattc tacgggcgcg gctacgcgct
    15961 gacccgcgcg gaacgcaagc aggcctatca aaaggcattg gaggacggca aggagctctc
    16021 ccggctgctt cttgagaggg cccgcagcgc aaccaaacga gatcggtatg cgtcagaaaa
    16081 agggtgatct tggttcttca gaccggaggt gttacatgat gttgcgcatg acggccttgc
    16141 tggcgatgac gctcagcctt gtacttgcgg acaggctgac gttgcgcgac ggcagggagg
    16201 tggaaggtac gtatctcggt ggcgacgcgc gtcaggttcg catggcggtc ggcgacaaaa
    16261 tcgagagtta tcgggtatcg gagatcacga gcctccagtt tggcggggcg acggcagcgg
    16321 caccggttac ttcgggttcg ggcgccacga ccaccctcgc ggcggagacg aaaccggcgg
    16381 acaccaagcc cgcggcggct gcacccgctt ccggaaagga aattcctgcc ggtaccgaag
    16441 tggtcattcg catgatcgac gacgtggatt cggaggtcag ccgcgtgggc cagacatata
    16501 aagcaagcct ggatgagccg gtgatcgtga acggcaacac gctgattcct cgaaacgtcg
    16561 acgtgaccgt gaagctgatc gaggaccagc agtcaggcaa gctgactgga cgcacggagc
    16621 tgacgctggc gctggtggac gtcaccgtgg acggtaagca ggtgcctgtc gtcacccagg
    16681 aagtcacaca ggcaagtgag tcgcgcaccg ggcggaccgc aaaagtagtt ggagggaccg
    16741 cggccctcgg cgcgatcatc ggcgcgattg ccggaggcgg taaaggcgcc gcagtcggag
    16801 cgacgaccgg cgcgggcgcc ggcgcggcgg tacaggtctt gacgaaggga cagcgtgttc
    16861 gaattccttc ggagacccgg ctgactttca cgctgaaaga gccggttcga ctctagcgtt
    16921 tcccatccgg taggatgatg gggacggaga gatcccatca tcgacacacc ggttgccatc
    16981 atcacaggag cgagccgcgg aatcggccgc ggaatcgcca ttgagctagc gcgcacccac
    17041 caggtgcttg cgacctacaa agggcgccgg gacgccgccg agtcgctgcg ggcggagacc
    17101 ggcgccgaaa tcttccagtg cgacgtcggg tcgcgtgacg atcggcaggc gctcctcgag
    17161 ttcgctcgag agcgttttgg acggcttgac ctgctcgtca acaatgccgg catggccccc
    17221 cgcgagcgtc gcgacatcct cgaagcaacg gaagagagct tcgacgaact gatcgcgacc
    17281 aacctcaaag ggccctactt cctcacacaa caggcggcgc ggtggatggc ggagcagggc
    17341 cgcgggcgca tcgtctttgt cacgtccatt tcgtcttaca cggcctcggt gaatcgcggg
    17401 gagtactgcg tgtcgaaagc cggtctgagc atggcggtag cgctctacgc gcagagatta
    17461 gcggcggccg gcgttcaggt gttcgagatc cggccgggga tcattcggac cgacatgatc
    17521 gcggcagtcg agcaggtcta cgaggagaag atcgccggcg gcctgctacc acagcggcgc
    17581 atgggtgaat ccacggacat tgcccgtgcg gtgcgcgcaa tagccgacgg cctgctcgac
    17641 tactcgacgg gccaggtgct gaacgtcgac ggcgggttcc atctccggag cctgtaacgc
    17701 cgacgccgtg ataatgtagt acttccactt tccagcgagg aaggagtatt tttctcccat
    17761 gaagaagggg ttttacggct ggtggattgt cgcggcttgt ttcctgactt tcggagtgtc
    17821 gacagggttc ccgtactaca acatcgcgtt ctttttcgac tacttccgcg acgaccatag
    17881 ctggccgatc agcttcatca cgatgggcgc tccgatcgcc gtgctgctga cgatctgggc
    17941 agggccggtc atagtgccac gtttcagccc gcgactgctg attctcgtcg gcaccgccat
    18001 gactttcctc gcatttcaat ggttcgcacg gctcagcggc tcgcggtggg agtacttcgg
    18061 cgcgtggtgc ttgtacatga tcgggtactt cctgtccggg ccgattccgc accaggtgat
    18121 catttcgaac tggttcaagg agaaacgcgg ccgggccatg gggatcacgt atgtcggcgt
    18181 ggcggtgatc ggttccattg gcaacaaact ggggccgtac ctggcgtccc agatgccgta
    18241 cacggacgcg ctcaagtaca tgggattcct gctgctgctc gcctggccgg tggcgctctt
    18301 cgtcctgaag gacaagccgc aagacgtggg ccagttcccg gatggcaagg cgacggcgga
    18361 ggaggttgca aagcccgccg tactggagcc gcccaagagc ttcgggttcc tgacccgaaa
    18421 atccgccttc tggctgctgt tggtgggaag cgcggcttcg atcggttcga tcgcggcggt
    18481 gaatttccac atgaagttcg tgttcgaggc tcagggcttt gtggaacagg cggcgcggga
    18541 ccagatttgg ggaacggctt cgttctgggt cctgtggtcg agcatcgcgg gacggttgtt
    18601 ggccggttac ctggctgaca aactgcctcg gaagtacgtc atgcttgcca cgtacatcgt
    18661 tgttgccctc gcgattccga cgctgttcct cgtcaagccg ggccacacgg ggttcctgta
    18721 tgtgttcgcg ctggtgttcg gattcgcgat gggtgcggac tacatgctga ttccgctgat
    18781 ggctgcagac cagttcggac tgtcaacact ggcccgcgcg atgtctgcaa ttttgccgac
    18841 ggataccatt gcgcagttct ggttcccgaa cttcgtggcg cggctgcgag atcttgttgg
    18901 cggcgactac cacatcgcca tgatggccgt cttcggaacc gctttcctgg gcgcgatcgc
    18961 catcggtatg ttgccgagac acggacccaa ggatgaggca cttcacgtac aagacgctcg
    19021 cggaactgcg gcgagacgct gaggtccatg gggcccggca catcgttttc gaggaggaac
    19081 cgagccgggt tcgagagatc ctcgcccgaa aagtaaaggt cgggccgctg atcatcggca
    19141 attcgatcac cattcacccg atggagggct gcgacggcac gccccaagga catccggacg
    19201 agctgacgtg gcggcgttat gagcgcttcg cggccggcgg cgcgaaactg atctggtttg
    19261 aggcgacggc ggtccgcgag gacgggcgcg ccaacacgcg gcagctttgg ctccacaagg
    19321 gcacggccga cgagttcgcc cgtctgctcg aaacgatccg ccgcattcat cgcgagcggt
    19381 gtggcacggc tgacgacctg atcgaacccc ttcaattgac gcattccggc cgatacagcg
    19441 tccccaagcg gattatcgct tgtcacaacc cggccgttga ccggaagacc ggcgtgccgg
    19501 cggactaccc ggtcatctcc gacgacgagc tcgagcggct tgaagacgat tacgtcgaga
    19561 cggcgcggtt ggcggtagcg gccggcttcg gggcggtcga catcaaggcg acgcacggct
    19621 atctgctgaa cgaattgctt tcagccaagc tgagggaagg ccgctatggc ggcccactcg
    19681 aaaaccgcac gcggttcatc cgcaacgtaa tcggcaagat ccgggctgcg ctcggcaacc
    19741 ggcttgtgtt gtgcatgcgg ctcgggtgct atgacggagt tccatacaca acggggcccg
    19801 gcaacacggg cgtgccgctc gagcatccga ttccttacct gtacggctgg ggcgtcgacc
    19861 cgaacgaccc gttccgggaa gacctgaacg aggtgaagca ggccatcggt tggttcaagg
    19921 agtggggtat cgaactgctg aatgtctcaa tgggatcgcc atactataat ccccacgtcg
    19981 gccggccgtt cgaaaaaccc gacgaaggaa attacgagcc gccggagcat ccgctgctcg
    20041 gcgtggaccg gcactttcgc atcgccggcg aattgcagcg ggcgatcccc gaccttccga
    20101 tggttggcac cggctatagc tggctccagc agtacgccat tcatgcgggc gcgcggaaca
    20161 tcgccgatgg aaacatccgg ctcttcggac tcggccggtg cgccatcgcg tatccagact
    20221 tcgcgcggga cgcgctggaa aaaggcgaac tggatgagca cagagtatgt aagacggtta
    20281 cgttctgtac ctacctgatg cggcagaaga accatccgct gggacagttc ccgacgggct
    20341 gcgtgccgtt tgacaaagat gtctacgggc cgatcatgaa agaggcgcgg gaagccagac
    20401 gcgccgccga atccaaaccg aaacactgag cacagacatg cgtgtaatcc tatccgttct
    20461 tttgagtgcg gccggggcat tgtccctgtc cgcggcgcag aatttcgatc tcgttgttta
    20521 tggtggcacc gccggtggcg taataaccgc cgtcagcgca gcccgggaag gagcgagcgt
    20581 ggctctgctg gagccgggcc gtcacgtcgg cggcatggtg acgggcggcc tcagccggac
    20641 cgaccacggt cggaaagaaa cggttggcgg ctactcgctc gaattctaca aacggctcgg
    20701 caagaagtac gggacagaga tcgagtggta tcccgagcct catgtggccg agcaggtcct
    20761 gctcgaaatg atgaaagagg cgggcgtcaa ggtcttttat ggacgccggc tccgcgaaaa
    20821 gaccggagtg cggaagaagg gccgccaggt cgttgagatc gtcatggaga acggcgctgc
    20881 gttccaggcg aaagtcttcg cggactgcac ctacgagggc gacctgatgg cgcaggcggg
    20941 tgtctcgtac acgtggggac gcgagggctc cagccagtac ggcgagtcgc tggccggggt
    21001 caggccgaaa gaccgaggcc accagttcga ctttccgatc tcggcgtacg acgccaaggg
    21061 ccgattgttg ccggagatcg gcacgcagcc gcggggcgag ctgggagctg cggacaagaa
    21121 agtccaggca tacaactacc ggctgatcat gagcaaggag ccgtcgaacc gccgcgccat
    21181 cgaaaagccc gccggctacg atccctggcg gtacgagctg ctggtgcgct ggctccaggg
    21241 attcgagaag cacaacggcc gtgcgccgcg catcaatgaa gtgctgttga tcggaacgtg
    21301 ccagggcaac aaagcggact tcaacaaccg tggcgccgtt tcgaccgatt acatcggcaa
    21361 gagctgggat taccccgaag caagctacaa acggcgcgcc gagatctggc gggagcacgc
    21421 cgactacaca aaaggcctcc tctacttcta tgtgacggat ccgcgcatcc cgaaatcatt
    21481 gcaggacgac atccggcagt ggggctacgc gaaagacgaa ttcacggaca acgacaattg
    21541 gccctaccaa ctgtacgtgc gcgaggcgcg ccgaatgaca ggcggcttcg tgatgacgca
    21601 gaaggacatc cagaccgagc tgacaaagcc cgacgcgatc ggcatgggct cctataacag
    21661 cgactcgcac aacgtgcagc gtttcgtgca ggcggatcgc accgtgcaga acgagggcaa
    21721 catggaggtt tcggtgaccc cgtatcaaat cccgtacaga attcttctcc cgaaacggac
    21781 ggaggcggag aacctgctgg tggcagtgtg cttctcggcc agccacgtga catattcgac
    21841 cctgcgaatg gagccggtct acatgatcat cggacacgcg gccggcgtgg cagctaagat
    21901 ggcgatcgac aagaaagtgg ccgtgcagga gatcgacacg aaggcattga ccgccaggct
    21961 acgagcgcag ggagcggcga tggaatagaa gcggccgtaa cgttggcggc cggaaagtcg
    22021 ccggaggggc ggcaacgccg ccccggttac gatagccgca ctggaccttc gccttccgag
    22081 agagtccgca ggattttgtc tttgtttcgt tcgatccgct cgagcgccga atcgagcgcc
    22141 acccagtaga acgcggcgcc cagagcggcg gcgatggcga tcacgctcca aaacgcagtg
    22201 tcgctctcga aggcgtagcg cgcaccatag gccaggagga tcggaattgc ggctgccggg
    22261 tagatcaaca gcagcattgc gcggagccgg ccggcactgg cggcgcctgt agagcgttcc
    22321 ggattgatgg cccgcggata gtagaccgag ctgacatttc cggcgccgag caggtagagg
    22381 gagaaggtca acgccacggc atacgcctcg gctgccttga cggcggtgag cggcatccgg
    22441 aaggcggccc atacgcagat aatggccgtg atctcgagaa acacgaaaaa cagcgcggca
    22501 atgttctttc cggcaaagac gcgcgaaatg ggcatgggca gcaggaagta gagctgcgcc
    22561 gcggaacggt cgaatccgaa tacgttccag aaggccacgt cgcccagaag cagcagggcg
    22621 tacccgctga ccatcgcgag gtgatcgccg ggcggcaagg gaccgccccc gcggcgcagg
    22681 atgatcggca ggaagacaat cacaccgaag gtaaacccca tcaggaaaac gagccggaag
    22741 cgtggcgtcc gaagcagcga ccgcaactcc ttctcgatga tgaccgccag tggatccgaa
    22801 aaaagcatac ccgggaccct atagaggcgg tccagccacg gacggctctc cccggaggtc
    22861 ctgcccctgc tttccgcggc ggcagcgtcg aaacgaaggc ttcgcgcgaa ctgccatcgc
    22921 ccgaagaaca acgccgcgat cgtccatccc agcaggacca gccagagggc tgccgttccc
    22981 tcccccgagg ccagccgggc agtggccgac cagggccacc acatttgcgc ctgcgccgaa
    23041 acgaaccagc gtaacggccc cggcacgccg gtgacaaaca acagttgcgg gacggcggcg
    23101 gcgagaagaa cgaacagcac gatcagctcg cgtacccgcc tccgggccat ccagcgctcc
    23161 atctgatttc gcagaccggc cgagacgagc agattgaaga caatgaacgg cagaaaggcc
    23221 agggtcccgt tgcggtgagg cagcgccggg ttgacaagca gtccggcggc cagccccgac
    23281 accagcagca tcatctcgat acacgtggac aagcggagca ggacttccac ctcaaacagg
    23341 cgccgcgccg gaatcggaaa aacgagcagc ttacgcaggt cgatcgaggc gcccatggaa
    23401 gccgtcagca gcggcgtcag ctgccaatag acaaagaccg caagcaggcc gttcggcaat
    23461 acgaggggta gcatcctccg gactcctggc tggctgacaa aaaagccgat tgtgacggca
    23521 agcatggccc acgtaccgta ccacgcgaga attgccagcg ccggcaggac gaagctcccc
    23581 atgccgtggc ctcggcgaaa attcagcagc gtccgccact gcgcccagag gatggccagg
    23641 atcgggtcgg acatgggcct acagccagtc gagcttctcg atggcgcgct cgccgcccac
    23701 tgtgcgcacg aacacgtcct cgagcgtgtc ggcgccgagg cgcagttctc cgatggcgct
    23761 ctccatcacg agccggccct cgtgtatgat cgcaacgcgg tcgcagagcc gttccacaac
    23821 ttcgagcacg tgcgaggtga ggaaaatcgt tgcgccctgg cggacctgat cgagcaacag
    23881 atccttcatc atccgcgctc ccaccgcgtc gacgccctca aatggctcat ccatgaggaa
    23941 cagctcgggc cgatggatta tagacgccgc cattgcgatt cgcttccgca tccccttgga
    24001 atactcggcg atcaactgcc ggggatttcc gtcaagctcg aacaggttga gcagttcgcg
    24061 cgcgcgttcc ttcgcaacgg gccttccgag accgtacatt cgcccgacga actcgagaaa
    24121 ttccgctccc gtcaaccggt cgaacaacag cgactcgtcc ggcacgagtc cgattttccg
    24181 cttgatggcc agcgcctgct cgggcatcgg gaggccgaga agttcgatcg atccggagct
    24241 cggcggcgtc agaccgatca acattcgaat cgtggtcgtt tttccagcgc cgttcgggcc
    24301 caagaaaccg aagaaacagc cacgggggac ctccagcgtt aacccgtcaa cggcggcttt
    24361 gtcgccgtag atcttgcgca ggtcggcgac ccggatggcg ggagaggcgt cctccataat
    24421 cctctctatc ctacttgata ccgccaagcg ctccgatagt gaagtttatg agccgcccgt
    24481 ccgatagaga attgggaaac tgcaatgttg accgtccgac ccacgcttgc gcgcgacaag
    24541 ctcttacaca gactggacct gctgccaccg ttctccccga tcctaaaccg ggcgctagcc
    24601 agtttgtctc aggagaacgt ctgcttcgcc gaggtcgccg acctgctcga aaaagacact
    24661 gtgctcgcag gcaacgtctt gcgtgtggtg aattcggccc tctacggcct gcaacgggag
    24721 gtcaactccg tacgccacgc catttcgctg gccgggattc accgggtacg aaacacggtt
    24781 ctcgctctct cggtcacgcg catgtggcgt catccggccg aagcgcacgg ctggaatacc
    24841 gtggcgttca gcctccactc ggcggcgacg gcgtgtttca gcgacctgct cgcccagctc
    24901 gtcccggttg agtatccgga aggcgcgttc accgccggcc tgtttcacga ccttggtaag
    24961 cttctcgtgg caaacgagct gcctcgggtc tatgaagtat tgcgaacgat ggacggccgg
    25021 gaggaaagcg cgatccttga gtgcgaactg gagcttgctg gaattcacca ctcggcgctt
    25081 gccgccgaag ctctggaacg gtggaagctg ccgcccgcga tccgggaagc agtcaggctg
    25141 caccatgctc ccggggacag ttccatcgga cccctcagcc tcggtcaggt tgtttgcacg
    25201 gccgaccgat gcgtcagggc gctggggctc gccgtcgcgc cgtcggcccc gccgggaata
    25261 cccgccgcac agatcctcga ccagtccggc gcaggtgcac atgcggaacg catcctggac
    25321 gaattccgcc aggaattgga cgtgcttcga gagttcttct gaaaatcgcg tccggttcag
    25381 gcaggcggga ggccccacgg cgcgcgcatc ggccgcgaga gcatccggtt ggcttcgtcg
    25441 tcgttgacta cgcgttccgc cgccgcatcc cacttgagat tacggccgag tttcagggcg
    25501 atgttgccga gatgagcgat cgtgatcgtg cgatgggcct gctcgattgg cgccacgggc
    25561 tccttgcgcg agatcacgca gtcgatgaag ttccggaagt ggtcatcgct cttgtagagg
    25621 tgtagctcgt ggtccgtgat gtcggagtag atgagattgt cgggtttact ttcgatgctg
    25681 ccgcgattca cccacaccga gccttcggtt ccctcgaacg ttactccgcc gcgagtcttg
    25741 ttggagatta ccatccggac gccgttttcg tagatcgcct cgaaggtgaa gtccaccgcc
    25801 gtgttgtaca gcccgtcgtc agaccactgc cccttggcgt tccggatctc gatcggaccg
    25861 gtaagctccg tccccatccc ccactgcgcg atgtcgggat ggtgcccgcc ccaatccgtg
    25921 atctgcccgc cggagtagtc gaggatccat cggaagttga catggcatcg cgccgggcag
    25981 tacggcgcct ccggcgcggg accctgccac atctggtagt tgaacccatc cgggaccggc
    26041 tccggctgcc tccgatgagc gtcgcgcgac aggtcgggcg ttccgcccgg cagtccgcac
    26101 gtcacagtct tcagcttccc gatccgtccg tttcgcacga gctcgcacgc tttacggaaa
    26161 cgggagtccg agcgttgctg actgccggtc tggaacacgc gcttgttgtc tcgaacggcg
    26221 ttgctcatgg cacgcccctc cgcgatggtg agcgagagcg gcttttcccc gtagatatcc
    26281 ttcccggctc gggcggccag gatcaccgga atcgagtgcc agtgatcggg cactgcgatc
    26341 agaacggcgt cgatgtcctt gcgggcgatc agatcccgga agtcctcgta catgtcgcag
    26401 cccttgtaga ttcccgaccg cttctcccgg ccgtagtgcc actcgacgta gctgcgcgcc
    26461 ggttcacggc cggcgatgtc gccatcccag tagccggcgc tcatccggtt caggtcgcac
    26521 accgcgacca cctgaacacg cggatcgcgg aggaaacccc gcatgtcgcc ggtgccctgg
    26581 ttgccggtcc cgatgacgcc catcgtgact cgttccgtgg ccgccggccg gttcgcctgc
    26641 ccgagggcgg aagcaggcac gaacaatgga gtcgccgctc cgagcggcat agtgttaagg
    26701 aaagtgcgcc gattcataag tgcattgtca ttctatccga caacgcgacc ggcgatgcct
    26761 gtgctctggc ggctactggg accggctgag gtcagccacg taaacctgcg tgtgcccgtc
    26821 gcggtcgctg gcgaaggcgg cgtagcgctc atcagcggaa aacgatgggt gcggatgggt
    26881 ccactgagga ccgtaaactg tgtcggtggc cgtctccatc caactgagag cgccgcggcg
    26941 tgcgctcctt gccgcctgaa agtcctccgc cagcgcgtaa cggctcttcg tccactggct
    27001 gccctggttg ctcgattcgg acaggcagat ccgacggcgc tggccactgg ccacgtccac
    27061 cagaaacagc ccttcatcgg gatggttcgt gtcgcacagc acccgggcgc cggcgcggtc
    27121 cgcgctgatg tgccaggcgt tgaattccgc gatgacgttc cgttcccgcg ttctccagtc
    27181 catccggcac aggagcttcg gccagacggt gtatatcagg tcgccggtgc gtcccaggaa
    27241 cgtctcgtgc acgatgaatt cgtcgttacc gtgctcgtag aggcattcca gcccggaacc
    27301 actccgcctc acccggtgca tccgcggtgc ggggtcggcc gcgaactcaa tccattccgg
    27361 ttccaacgga tgaaactgcg gatggatcac cgtccgcgga aacgcgatga cgtgccagcc
    27421 ttctccattg catcgaccca cggcgatacc gaagccggag tcctgtctga tggccgcggt
    27481 gatccattcg cccgcggcat cgagcgagca ctcgcccagt tgcgcccccg gaaactcgtg
    27541 gaccctgtgc tccctcaggc tcgttcgctc gactgcccaa acgccaccgc cgcgcacgaa
    27601 caggatgcgt tcgccgtctg gatgaatcag cggcgaaaag gggtgaatcg gttcgccatc
    27661 cgtcagttgc cggatcccgc cgagtggaaa tcccgcctct aacagttgag cctggcccgt
    27721 tctgtacgaa gtgaaaatga tcgcctggcc gtccggggta aacgaactgt ggaggaaata
    27781 cgttgggtgg ctgatggaca ggtgccgcgt catctggtgg attgttgcac cagtgacccg
    27841 gtcccgaaac tgcgagtgct ccgaggggta gacgcagccc ttcagcgact cgctcacgcg
    27901 ccgatgtccc ttgctttcgg aggaacggga tgtgccagcg cggcaacggt ctgcgcagtc
    27961 cgaagacacg ttctaactaa gtcgcgtttc atggtgcgtc ccagaccgaa tccgtgtagg
    28021 ataaagcgag tatcggccta tgataactcg attttctctc agaataccgc atttcctcag
    28081 cctgatgttt ttggccgcgt gtctcctgct ggcgggttgc tccgaggcat caaattcggc
    28141 tcgcaagagg ctctatcaca tgggggagcg cgtcgaagcc ggcgcgcttg tgtacacggt
    28201 gctcgaagcc gaatggcagc cacaactcgg cgaaggcgcc aacgctcgta tccctcagca
    28261 tcagtttctg atgctccgtg tgagcatcac gaatagcagt attcgcgaga tcagtatccc
    28321 gcagttgcgg cttgttggat cccgccgtga ctctcacgcg gaactgacgg acggcgaccg
    28381 ggtcccgaac tggctcggca tgctacgctc cctgaaaccc gccgacacga tcgagggtcg
    28441 gatcctgttc gatgtgcctc gtgaagattt ccagttggaa gtcagcgacg atgcccttga
    28501 tccggagcag agaaacgtcg cattgatcga ggtgccgctg cgatttgagt cggggaatgc
    28561 cgacctgccg cagcgcactc gttaggtgag cggaagcgat gacgcaaact tggctcggct
    28621 ttgtcctgct gttaacgctc cagcaggcgc cagttgggac gttcgggata accagtccaa
    28681 cgctgcatca gttcgaggac gggccggcga ttccggccgg acaccgcttc atccccggag
    28741 aaacggtgtt cttcagttgc caggttcagg gctaccgggc tacgccggaa cagaaggtcc
    28801 gcctaagcta caaggtcgac gctgtcgacg ccgacggcgt cctgctcgtg gccacggaca
    28861 ccggcaagat tgacaccgaa cttgccccgc aagacaagga atggatgccc aagatccgcc
    28921 gcgccatcct catccctccg cacgcgccgc cggggcagta ccggatcgtc accaccgtca
    28981 aggacgaggt cggtgggcag gaggcgaaga ccgagctatc gttccaggtc cacggcaggc
    29041 gcgtggaacc gagtgacact cttgtcgtcc ggaatttccg cttcctgcgg agcgaacagg
    29101 atgacaatcc gcttcccatt gccttctacc gtcccggcaa cacgctgtgg gcccgttttg
    29161 agatgaccgg cttcaaaatt ggcgagaaaa accgcgttca cgtggagtat ggaatcgcga
    29221 tcctcagtcc ggacggaaag caactgttcg cgcaggaaca agccgccgtc gaagaggatt
    29281 cgcccttcta cccgaaacgg tatgtgcccg gccagctcag cctgaacatc cagccgaaga
    29341 caccgcctgg cgagtacacc ctcgtggtct ctgttcggga ccaggtcggc gcccagacaa
    29401 tcgagacacg ccagcctttc cgcatcgagg agtaagaggg tccgctgttc cggcgagccc
    29461 tccctcgctt cgccgactat tgcactgtga aggtcaaagg ggcgctgctt acgctctgcg
    29521 cgttgtaagt cgaagtcgcg ctcgcctgcc agttacctct cggatcgcgc ttgccaatcg
    29581 tgtaattcca aaaagcctcg ccggccgaat tggtggtgat ccgctttgac tttgtcgaac
    29641 tgccgttgga cttgacgatc ttgaaggtca ctgccgcacc tgccgccgga gagccatcgt
    29701 tcgtcacggc tgccttcagt ttcacgatag aaccggtctg gtacggtccc gtactgagaa
    29761 acgacagctt cacggccacg atcggtggag gcgcgggaac cgtcgcattg gcggtaccct
    29821 ctccggagta acttccttta gtcgcggcgg cgttcacttg ataagtgcca gcgccggtac
    29881 cggaaggagc cgtctttgtc atcgtcgtcg cgcccgtctc acccgggttg actgtgaccg
    29941 cgttcagcgt gaaggaactg ccccaccccg aaggcagagt gctgctcatg ttgaaactgc
    30001 cggccgggca tccggtagaa tcgttattct tcacggatac ggtgtagtta gcactgccac
    30061 ctggctgcac cgtcgggttg gacggagaga tcgtcacggt tggagccgcg gaggtgcacg
    30121 ggacggcccc gtagttgacc tcaaccgtca ttccggagtc actgacactg acgaccctca
    30181 ccgagacgtt ggagtacgga tcggcccatg tctgtccggc cagcagggcc ggatcgttga
    30241 acgatgttgt cgacggcgtg aagtcagcca ggtgcgtttt cgtccccgtc gtcgaatcct
    30301 cgtagtgaat gagcgcgccg ccgaagacct gcgatccgat cgcggagtcg tagttgccga
    30361 tggctcggcg cgactcgatc cagagccaag cgtcgttgcc cgtaccccgg cgaactttca
    30421 gggccttcag cccgccaaag ctctcgtagg gaaggatggt gtacagccca ctggtctcga
    30481 cgacctggta gttggcgccc aacgcaagcc agccgagttg ctgttcggca tggtgagcac
    30541 tgtaatagcc aaggttccac gatcccatgg tcgaaaacag atcgccgtac tccgtgagtg
    30601 tgcccgcgtc accgagtgcg ccaagcggaa cgcgggcgcc ccccgcatca gtgaactcgc
    30661 gggaactcgc atggtgcatg gacaggttgt ggccgagttc gtgagtggcc agctcgacgc
    30721 cccagaggcg gttggccgtc tggtcggatc gctcccacac aaatgacgcg ttgaacccgc
    30781 cgtcctgcgt tccgctgttc cagcagccca agtctccgag cccggcccat ccgcaagcgc
    30841 cgttcggtgg aaagatcagc atcacccgcg agtagcttgg gaagtagact tccgcgtcag
    30901 cctgttgtaa cgcttccgcc ttcaagccgt tatagtcgca gtatgtctgc ccggaggagt
    30961 tcttgttgta gtcttggctc agcaggtatg gaccgaccac gttaccgccc gaagccggcg
    31021 cccaggtctg atgatccgag ctttcatacc agaagttgtc gacggagcgg ttcggcgtgg
    31081 catccttggt agtgaaatcg ttgccccaga ggattccctt catcaactcc tgatccacgc
    31141 ccgagctgag cgtgtagctt gccaggttca ccagaatagc gacaatgttc tgagcgccct
    31201 tcgtactgca tgccgccgcg acggcgtccg tgccgagaat actgccgtct tgcgccgcta
    31261 cccggttgcc cgcccggatg ccgcgaacgg aaacaatgtc gccacccttg agccccttcg
    31321 gctcggaccc ggcgaagtga atctccaggc tctcctgacc gtaccgcagc cgctgaatcg
    31381 attggtggcg ggtcattgtc gggtcatcgg tgatctggta ttcgagcact cctttccagg
    31441 atccgtgtgc ctcgagtacc gctgccgatc cgggaaacgc ccggctcagt gtgccgagca
    31501 aatcctcggc aaacgcgagt ttgagcgctt ccgccgggtc aacctcaatc aaagccgcca
    31561 gcgcttcggc tcgctggcca atcaccgccg cagcctcgct tctgacctgg g
//
LOCUS       DATIFQ010000004        17096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2108572, whole genome shotgun sequence.
ACCESSION   DATIFQ010000004 DATIFQ010000000
VERSION     DATIFQ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17096
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108572"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VLE22_00220"
     CDS             complement(<1..562)
                     /locus_tag="VLE22_00220"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HSB12846.1"
                     /translation="MQRRDFLKSAAVVAAPGSVGSASAAPKKALMKAGTQHNSSDEVL
                     KVMAAFGVNHICSTLPSVRMDENWTVEGLTKLRERVESHGIKLDMVPLPLSSGYITKA
                     ESPNIVLGKSPERDREIDTLCQMIRNVARAGIPAVKYNLTILGVVRSDPTPGRGGAQY
                     STFNYDKTKQDPPLTEAGRVTEEMFWE"
     gene            complement(586..885)
                     /locus_tag="VLE22_00225"
     CDS             complement(586..885)
                     /locus_tag="VLE22_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12847.1"
                     /translation="MDERKFFNETPTTKPTQLQCPFCRTTESYELHWLVRKKKDRVPP
                     GADERDRAKFQKAQSYMVLLDDKVACRNLRCRKRFDISGIKTMAFLVEGAGLPRG"
     gene            complement(888..2195)
                     /locus_tag="VLE22_00230"
     CDS             complement(888..2195)
                     /locus_tag="VLE22_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSB12848.1"
                     /translation="MTKDEILLAHREHLFPSVFHYYKEPLVVSHAKNQYVWDVDGNQY
                     LDFFGGIVTVSVGHCNDEVNRKLHAQMDRLQHVSTVFAMEPQAALAKKIASITPEGKL
                     TKSFFTNSGTEANETAILAARCYTGSSEIVALRHAYHGRSALMMTATGHHNWRLGGTP
                     QPGFVHAHNAYCYRCPFGLTYPECGVQCARDVEELIRTTTSGRVAGFIGEPIQGVGGF
                     ITPPKEYFGIVAEIVRRYGGLIISDEVQTGWGRTGNKWFGIEQYGVTPDIITSAKGLA
                     NGSPIGLTVARPDVADSVKGLTISTFGGNPVTSTAAKAVIDFIEEHDLRTNTAETGAY
                     LRGKLEELKEKHALIGDVRGMGLLQAIELVDDRATKAPATAAIAQLMELTRQNRILVG
                     KGGLFGNVLRVSPPMNIGKSDVDEFVRLLDRSLTQCGAPAAGR"
     gene            2400..4181
                     /locus_tag="VLE22_00235"
     CDS             2400..4181
                     /locus_tag="VLE22_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSB12849.1"
                     /translation="MQLTWIDWSVVALYFAINLAVGFYYKKRAGKNVDEFFLSGRDVS
                     WWLAGTSLVATTFAADTPLAVTGMVARYGVAGNWLWWNFVASGMLTVFFYARLWRRAG
                     VMTDIEFTEIRYAGKPAAFLRGFRALYFAIPINCIVLGWVNLAMVKILMLILGIDKLP
                     ALFIVLGLIALTSFISTISGLWGVLVTDMIQFIIKMSMVIVLGVAAVAAVGGIDAMKA
                     KLAAIDAAKGLETGGTGSLLSFIPDLNSPWMPLITFLVYISVNWWAVWYPGAEPGGGG
                     FVAQRMFCARDEKNSLLATLWFNIAHYSIRPWPWILVGLASVILYPGLADPETGYVRV
                     MIDHLPPSLRGLMVAAFAAAFMSTIATQLNWGASYLVNDFYRRFLRKDASQRHYVRVS
                     QAATVALAIISAVVTFFMGSIEAAWKLLIVTGAGTGGVLLLRWYWWRINAWSEVSAMI
                     AAFVVSVSLQTVWGLDSARPLDFAWMMLITVGVTTAVWILTTFLTKPESSETLVAFYR
                     RTRPSGAGWGPVSRLAPDVKPARDGLANLVCWIAGCVLIYGSLFGIGKLILKDYALGA
                     LLLAVSLAAGAVIYRDLSRRGWSAVAE"
     gene            complement(4203..4718)
                     /locus_tag="VLE22_00240"
     CDS             complement(4203..4718)
                     /locus_tag="VLE22_00240"
                     /inference="COORDINATES: protein motif:HMM:NF020553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HSB12850.1"
                     /translation="MITPITPETRVGDFLDAYPALEESLIASVPALEKLRNPVLRKTV
                     TQVITLEQAARLGGLSVCDLVQKLRNAAGQTDEDEPSGMHPGAPVWLCVDRIRHNIDA
                     TSMLERGVHPIGTVRECVATLAPGEIIRLTSGFRPAPLIDLMSRSGLAVYSMETSPGS
                     HETFFARPMPE"
     gene            complement(4715..6076)
                     /locus_tag="VLE22_00245"
     CDS             complement(4715..6076)
                     /locus_tag="VLE22_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HSB12851.1"
                     /translation="MIRRGVTKVMVEGAIPLLTWNQVRISENWPGKENRVSELIDNRA
                     HRVRTLKDIIKHLHSGQAPEQVKDRLRQIVKETDYSEIVAMEQELMAEGMPVEEITCM
                     CDLHSQVTREVLVQLPPRTVPPGHPVDTFRRENEALRGVLLKMRQAMAEIRALAADAD
                     PGASLLCWRQAYNDLMDIEKHYQRKEHTIFSCLEQHDISGPSKVMWAKDDEVREMLKG
                     LGAALAERGAGADEWKLVADTAGESLAAAVEEMIYKEDNILLPMCLDTFTEDDWAEIW
                     AASPRYGWCLVEPREGYRPPESKIRDAVRLSSAEAVQLPTGNVTLDQLTAIFSTLPVD
                     LTFVDADDRVCFFSEGPDRVFARSKAIIGRKVQHCHPPRSVDVVDRILSDFREGRQSV
                     AEFWINFQGRFVHIRYFAVRDRDERYLGTLEVTQDLTKVRALEGERRLLEYDAPTPAG
                     VGQ"
     gene            6091..7038
                     /locus_tag="VLE22_00250"
     CDS             6091..7038
                     /locus_tag="VLE22_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HSB12852.1"
                     /translation="MTPLSPADLDTAIAFPFLPFRGGRLDLDAHRLNAAFLARHCYLD
                     GGRRRVIAIGGSSLLHHVTPAEQLDVVRILCEEAGERARVISAVLPTPLCEAERIVRA
                     QMALPRPPDAVMFLPMIGGYNPEGVGRDLHRFCDRLGRETGTRFILYLRDAGLREVYC
                     RLVRESEYVLGVKIGTSHDDVAPAREALGGEGAVVWGMGDLCTRAVRLGARGHTSGTS
                     LLSLRASDEINNAQRRGDFEAAEEIEEELRELEEIRFMQGRIYNYSALAEALRIAAFD
                     DVEPGDGGPFNAPPPPEICARLERIVDRLRPYHFPPRSP"
     gene            complement(7119..8588)
                     /locus_tag="VLE22_00255"
     CDS             complement(7119..8588)
                     /locus_tag="VLE22_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSB12853.1"
                     /translation="MKLVLLLAAAVSINPEAYSQTKYAATWESVDSRPTPEWFTDARF
                     GIFIHWGVYSVPSYAPVGQYAEWYWNALQNGPTIKGKPNPTWQFHAKNYGERFPYQDF
                     APAFRAEMYDPDYWAEVFEKSGAKYVVLTSKHHDGFALWPSSHASAVWKRPWNAVETG
                     PKRDVLGDLTAAVRKRGLRMCFYYSLYEWFNPLWLSDRKRYVTEHMHPQFKDLVTRYQ
                     PSLIFSDGEWDMPGEDWKAPELLAWLFNESPVRKDVVVNDRWGKGSRHQHGGYYTTEY
                     TAGLQDISHPWEESRGMGFSYGYNRAEQLGDYRTGRELILMLIDLVSRGGNFLLDIGP
                     TADGRIPVIMQERLARMGGWLKVNGEAIYGTRPWRRTRQWSAGEQPKLETGEFMTKYE
                     ITDYVEQRKPGQAVIEAFFTVKGDSLYAIVPGWPGGKLTVRDIDAPATMRAKLLGADG
                     DLKWSRAGKDVVVEMPVPPGDAAKAQYACVVRLSGVNTR"
     gene            8641..11211
                     /locus_tag="VLE22_00260"
     CDS             8641..11211
                     /locus_tag="VLE22_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HSB12854.1"
                     /translation="MRNATLILTIVLTLTLSIAHAQGPITSPEKFFGFQLGADRKIAR
                     WDKIVQYYELLEKESAGRLKVVNMGPSTMGNPFLLVIISAAGNLSKLDRLREVNASIS
                     DPRGLTEQEVRKLVGEGKAVVCQTMSLHATEIGGTQMAPELAYDLLSRRDDETRQILD
                     NVIFLMVPSFNPDGAIMVADWYQKTLGTEYEGGSLPWLYHKYAGHDNNRDAFQQNLVE
                     SQYVAKIMFRDWVPQAYIDHHHMGSYGARIYVPPYAEPMRPLSDPLVWREMSWYGAHI
                     AYKEEEGGYSGVLNMAQYSGWGHFGFHWITPFHNIAGMLTESASAKLATPLYIHPHQL
                     KGEVRGLPDYNVQTTFPNPWTGGWWKLRDIVERQKVSAWALLDLAARNRETVLWNAYL
                     KAKRQTERGAQGKPTAYVIPKSQHDSLAAVMLVNKLLLQGIEVKQAKNSFTSATGMTY
                     PAGSWVVPLAQPKMGVIRYLLGRTRYPDNDWTRARDGSPIRPYDMAADTMFEFMGVRV
                     DPFEDGLPGDLPKLTGVLQPSGKVEQNRNGYSFDGRLNESFKALNLLLDKGVGVRRVE
                     KEGDGLRAGDFVVSPGSEALLKEVAQATGVDFTALRGRPAEGTHELKRLRVAMYQRYR
                     GGNIDEGWTRLLLEQYHFPYTTLMDAEVKKGGLNEKYDLIILPDDSTGAIVGETTESS
                     HRDEPFPPEYRSGIGKEGVDALKTFVEKGGTLVALGGASNFAIEKLGLHVRNVTAGRS
                     SKEFWCPGSTLKVQFDNTHPLAWGMPAEGLSLFVGGSPAFEILPNEHNDWYEVIVRYA
                     DRDLLESGWLLGEETLAKKAGMIAVKHRDGRVILMGSRPQHRAQTHGTFKLLFNALIR
                     "
     gene            complement(11235..11663)
                     /locus_tag="VLE22_00265"
     CDS             complement(11235..11663)
                     /locus_tag="VLE22_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB12855.1"
                     /translation="MILLDANLLLYAVNKDAPLHSKAKTWIEGAISGPDTVAVCWSVL
                     LAFLRLTTRHGVFRNPLSPEHALDLVAAWLDQPTVTVVHPGPRHLTILRQLLGSLGTG
                     GNLTSDAHLAALAIEHGAELCSCDGDFAHFPGLHWKNPLA"
     gene            complement(11660..11923)
                     /locus_tag="VLE22_00270"
     CDS             complement(11660..11923)
                     /locus_tag="VLE22_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12856.1"
                     /translation="MRTTVTLDPDVERLVRAAMRARGLSFKQALNQAVRVGLSSARTS
                     RGRRFAQKTYSLGPEQNFRWDKALAAAEALEDEDLSRKLSLRK"
     gene            complement(11966..13540)
                     /locus_tag="VLE22_00275"
     CDS             complement(11966..13540)
                     /locus_tag="VLE22_00275"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HSB12857.1"
                     /translation="MRGSPEGVFIIQRAGWGLYDWWSMPIQKFLLSILFCAVALSSSA
                     SADVSLPTLLADHMVIQRGLPVHIWGKAAPGEAVNVTLGDDSTSTTADSLGFWSVYLP
                     PREAGGPFELTVKGKNTISLKDVLVGDVWVASGQSNMELPVSASANADNEIAAATHPR
                     IRLYRVKNNVASYPLLDAEAEPWTPCTPETARDFSAVAYFFGRNLEDKLAVPIGLIET
                     AWGGTPADAWTSLRSISADASLMPVFAEWSRMNDDFAVSSLRREKQLDEWRQAVEKAK
                     AAGKEPPGFPWEPNERGCWAPAGLFNAMIAPLTRYPIRGAIWYQGESNAPAARAPLYA
                     RLFRTMIQDWRRAWGQGDFPFLFVQLANYRPGPDTKWPEVREAQRQTLALAKTGMAVT
                     IDIGDPADIHPKNKQDVGARLALAARAIEYGERVEYSGPLFRQATREGKAIRVWFDHA
                     GGLAAKGGSLRGFEVAGADHKFVAAEARIDGSTVIVSNSSVAEPRLARYGWSDDPGSN
                     LYSAAGLPASPFRSEE"
     gene            13539..14621
                     /locus_tag="VLE22_00280"
     CDS             13539..14621
                     /locus_tag="VLE22_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12858.1"
                     /translation="MASAPFGNDPAKIGRYRAFWKRDDVRRPLVGFSLVGWFPLQEFA
                     ACRAWKNTEYLTLDMIDPEAFLDDHLRMLREGEILDDDVIRGACPGQVALPWLPAMLG
                     CKMRILPHNVMGEEQHLPWEEALAIRLDLDNPWFRKYQEFADVLVRASNGAFPVSHSA
                     EIGPSDLHATLRGHTTGVLDLADEPENSACLLWRMGEIFRQVTEEFWKRVPLFQGGYF
                     DAQYSLWAPGPIIRMQEDASAVYSPALYRKFVQPVDRMLAAHFPSSFIHLHSTSMFLL
                     DAFLEIAEIQCFEVNNDALGPPVAAMVPHFCKIQRAGKPLLIRGSFTTEEMRLLMDSL
                     EPRGLFLYIMVNEMREIDELRLLVGL"
     gene            14713..15408
                     /locus_tag="VLE22_00285"
     CDS             14713..15408
                     /locus_tag="VLE22_00285"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HSB12859.1"
                     /translation="MSDTDGILLGTDGRPRCFWSASAPDYARYHDDEWGYPVTDDRRL
                     FEKICLEGFQSGLSWLTILRKRQDFRRVFHDFDFERVARFSSRDVERLLKDASIVRHR
                     GKILAAINNARRARDLAREFGSLSAYFWTYEPAPSTRPGLLTWEVLRGMTTSPEATAL
                     SKDLKKRGWSFVGPTTVYAFMQAMGLVNDHLNGCFVRPLAEAARRKSGRIVVRSSDPV
                     PAPPGRTARDRRR"
     gene            complement(15347..16852)
                     /locus_tag="VLE22_00290"
     CDS             complement(15347..16852)
                     /locus_tag="VLE22_00290"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HSB12860.1"
                     /translation="MSTQHPSHGANGVRDLRSALTFLAGFPQHLHKTQEPVDPYLELA
                     GVYRLAGAGTPIAPPTRIGPAMLFENVRGYPEARILVGVFGSRERTALLLDTTVDRLP
                     FRLMEAVERPVPATVVEGSTAPCQEVIHRAPVDLTRLLPVPTGTAKDAGPYFTLGLLR
                     GEDPETGESDVTIHRMCVLGPDRLSVYFVPGRHIGEFYRKAEARGESLPVSVSIGLDP
                     AVYVAACFEPPTTPLGFDELSVAGALRGRPVELASCVSVKAKAVARAEIVIEGEILPG
                     ERVREDERTGGGWAMGEFPGYLGRAQMSVPVIRVRAVTHRRQPILQTLVSPGAEHTNL
                     VGIPTEASILRFVEDSMPGRLLNAYAHPAGGGKYLAILQFRKAAPSDEGRQRQAALAA
                     FSAFPELKHVILVDEDVDIYDTNDVLWAMTTRYQGDASTAFIPGVRCHPLDPSQSPEF
                     SPSIAGEGISCKTIFDCTVPFRMKDRFVRAPFSDADLARFFPEGPGPGPKI"
BASE COUNT         3012 a         5596 c         5117 g         3371 t
ORIGIN      
        1 gttcccaaaa catctcttcg gtgacacgcc ccgcttcggt gagaggagga tcctgcttcg
       61 tcttgtcgta gttgaacgtg ctgtactgcg cgccgccgcg ccccggagtc gggtcggacc
      121 gcaccacacc gagaatcgtc agattgtact tcacggccgg aatgcccgcc cgcgcgacgt
      181 tccggatcat ctggcacagg gtgtcgatct cgcggtccct ttccggactc ttgcccagca
      241 cgatgttcgg actctcggcc ttggtgatgt agccggaact cagtggcagc gggaccatgt
      301 ccaacttgat gccgtgcgac tccacgcgct cccgcagctt tgtcagtcct tccactgtcc
      361 agttctcatc catccgcacg gagggcaggg tgctgcagat gtggtttaca ccgaacgcgg
      421 ccattacctt cagcacttcg tccgacgagt tgtgctgggt gccggccttc atcagtgctt
      481 tctttggagc cgcggacgcg ctccccacac tgcccggtgc cgccaccacc gcggcactct
      541 tcaggaaatc tcgtcgctgc atggaattct ctccgtggga cttttctatc ctctcggcag
      601 ccccgccccc tccaccagaa acgccatcgt cttgatgccg gagatatcga agcgtttccg
      661 gcaccggaga tttctgcacg cgaccttatc gtccaggagc accatgtagc tctgcgcctt
      721 ctggaatttc gcccggtccc tttcgtcggc acccggcggg accctgtctt tcttcttccg
      781 cactagccaa tggagctcgt agctttcggt cgtgcggcag aaaggacact gaagctgcgt
      841 cggcttggtc gtcggtgttt cgttgaagaa cttgcgttcg tccatcgcta cctcccagcg
      901 gccggcgcgc cgcattgggt gaggcttcgg tcgagcaggc gcacgaactc gtccacatca
      961 ctcttcccga tgttcatcgg cgggctgact cgcagcacgt tcccgaacag tccgcccttg
     1021 cccaccagga tccggttctg ccgcgtcagc tccatcaact gtgcgatcgc ggcagttgcc
     1081 ggagctttcg tcgcgcggtc gtccacgagt tcgatggcct gaagcagccc catgccgcga
     1141 acatcgccga tcagcgcgtg cttctctttc agctcttcga gttttcctcg cagatacgcg
     1201 cctgtctccg ccgtgttcgt ccgcaggtca tgctcctcga tgaaatcgat taccgccttc
     1261 gccgccgtcg aggtcaccgg gttcccgccg aacgtcgaaa tcgtgaggcc cttcaccgaa
     1321 tcggccacgt ccggccgcgc cacggtcagt ccgatcggcg acccgttggc gaggcccttt
     1381 gcgctcgtga tgatgtccgg cgtgacgccg tactgctcga ttccgaacca tttgtttccg
     1441 gtgcggcccc agcccgtttg tacctcgtca ctgatgatca gcccaccgta cctcctcacg
     1501 atctcagcga caatcccgaa gtactccttc ggcggcgtga tgaaaccgcc cactccctga
     1561 atcggctcgc cgatgaaccc ggccacccgg cccgaagtcg tcgtccggat cagttcctcc
     1621 acgtccctgg cgcactggac cccgcactcg ggataggtca atccgaacgg gcagcggtag
     1681 cagtacgcgt tgtgggcgtg gacgaatccc ggctgcggcg taccgcccaa ccgccagttg
     1741 tggtgcccgg tcgccgtcat catcagggcg gatctgccgt gataggcgtg ccgcagcgcg
     1801 acgatttcgc tcgatcccgt atagcagcgc gccgcgagaa tcgccgtttc gttcgcttcc
     1861 gtgccgctgt tggtgaagaa cgacttggtc agcttgcctt caggcgtgat cgaggcgatc
     1921 ttttttgcca gcgccgcctg gggctccatc gcgaacacgg tcgaaacgtg ctgcaaacgg
     1981 tccatttgcg cgtgaagttt ccggttcact tcgtcgttgc agtgtccgac gctgaccgtt
     2041 acgattccgc cgaagaagtc cagatactgg ttgccgtcga cgtcccacac gtactggttc
     2101 ttggcgtgtg agaccaccag cggctccttg tagtagtgga agacagaggg gaagaggtgc
     2161 tcgcggtgag cgagcaggat ttcgtccttc gtcataggct tgggagtcag tgtaccaagg
     2221 accgtctcca cttcgccggt tgtggaggcc tggggataga catttcgtgt tgatgcaaaa
     2281 ctccgggaca gacaccaatt ccgcaaacag cgcgaaattc ggagtcagtc ccgggttttg
     2341 cgcaagagcg gcgaaactgg agtcagtccc gggttttgca gtatcttgtc ttattcccca
     2401 tgcaactcac ctggatcgac tggagcgtcg tagccctcta cttcgccatc aacctcgccg
     2461 taggcttcta ctataagaaa cgtgccggga agaatgtcga cgagttcttt ctttccgggc
     2521 gggacgtctc ttggtggctt gccggcacct ccctcgtcgc caccacgttc gccgccgaca
     2581 cgcccctcgc ggttaccggc atggttgccc gctacggggt cgccggcaac tggctctggt
     2641 ggaacttcgt cgccagcgga atgctgactg tctttttcta tgcccgcctc tggcgccgcg
     2701 cgggggtgat gaccgacatc gagttcacag aaatccgcta cgccggcaag cccgccgcct
     2761 tcctacgcgg cttccgcgcg ctctacttcg ccatcccgat aaactgcatc gttctcggct
     2821 gggtcaacct ggcgatggtg aaaatcctga tgctgatcct cggcatcgat aagcttccgg
     2881 cgctgtttat cgtgctgggc ctgatcgcgc tgacctcgtt catctccacg atctccggcc
     2941 tgtggggcgt gctggtcacg gacatgatcc agttcatcat caagatgtcg atggtcatcg
     3001 ttctcggtgt cgcggcggtg gccgctgtcg gcggtattga cgccatgaaa gcgaagctcg
     3061 ctgccatcga cgccgccaag ggcctggaga ccggcggtac gggatctctg ctcagcttca
     3121 ttccggacct caactccccc tggatgccgc tgatcacctt tctcgtctac atctcggtga
     3181 actggtgggc ggtgtggtac cccggcgccg agcccggcgg cggcggtttt gtcgcccagc
     3241 gcatgttctg cgcccgcgac gaaaagaact cgctcctcgc caccctctgg ttcaacatcg
     3301 cccactattc catccggcca tggccctgga tcctagtcgg cctcgcttcg gtcatcctct
     3361 acccgggtct cgccgatccc gagaccggct acgtgcgggt catgatcgac cacctgccac
     3421 cgtcgctccg gggcctcatg gtcgccgcgt tcgccgccgc cttcatgtcc accatcgcca
     3481 cccagctcaa ttggggcgcc agctatctgg tcaacgattt ctaccgccgg ttcctgcgca
     3541 aagacgcctc ccagcgccac tacgttcgcg tatctcaggc cgccacagtc gcactcgcca
     3601 tcatctccgc tgttgtgacg ttcttcatgg gttccatcga ggccgcatgg aagctgctga
     3661 tcgtcaccgg cgcaggcacc ggaggggttt tgctgcttcg ctggtactgg tggcgcatca
     3721 acgcctggag tgaggtctcg gccatgattg cggccttcgt cgtctccgtc tccctgcaaa
     3781 ccgtttgggg actcgacagc gcccgcccgc tcgatttcgc ctggatgatg ctgatcaccg
     3841 tcggtgtcac caccgccgtc tggatactga cgacgtttct gacgaagccc gagtcttcag
     3901 aaacgctcgt cgcgttttac cggcgcacgc gtccctccgg cgccggatgg ggaccggttt
     3961 cccgcctggc gccggacgtg aagcccgctc gcgatggcct ggcaaacctc gtctgctgga
     4021 ttgccggctg cgtgctgatc tacggcagct tgttcggtat cggcaagttg atcctcaagg
     4081 actacgcgct cggtgcgctg ttgcttgcgg tttctcttgc cgccggagcg gtcatctacc
     4141 gcgacctctc tcgccggggc tggtcggcgg tagctgagta gggttcagtg aagtgccgcg
     4201 acttactcgg gcatcggccg tgcaaagaaa gtttcgtgtg acccgggtga cgtctccatc
     4261 gagtacacgg cgagaccgct gcgcgacatg aggtcgatga gcggcgctgg gcgaaagcca
     4321 cttgtgagcc ggatgatctc tccgggcgcc agagtcgcaa cgcactcgcg gaccgtaccg
     4381 atgggatgca ctccccgttc caacatgctg gtcgcgtcga tgttgtggcg gatacggtcc
     4441 acgcaaagcc agaccggagc ccccgggtgc attccgcttg gttcgtcttc gtctgtctgg
     4501 ccggcggcat tacgcagctt ctggaccagg tcacagacgc tcagaccacc gagtcgtgcc
     4561 gcctgctcga gtgttattac ctgcgtcacc gttttccgca gaaccgggtt tcgcagcttc
     4621 tcaagcgccg gcacgctggc gatcaggctc tcttcgagcg ccgggtaggc gtcgaggaag
     4681 tctccaactc tcgtctccgg agtgattggc gtgatcattg tccgactcct gcgggggtag
     4741 gcgcgtcgta ctcgagcagc cgacgttccc cttccaatgc ccggaccttc gtcaggtcct
     4801 gcgtcacctc gagagtcccc agatagcgct cgtcacggtc cctcacagcg aagtaacgga
     4861 tgtgtacgaa ccgcccttgg aagttgatcc agaactcggc cacactttgg cggccttccc
     4921 ggaagtccga caggatgcgg tcgacaacat cgacgctccg tggcgggtgg cagtgttgca
     4981 ctttgcggcc gatgatcgcc ttactgcggg caaagacccg gtccgggcct tcggagaaga
     5041 aacagacgcg gtcgtccgcg tcaacaaagg tgaggtccac cggcagtgtc gagaagatgg
     5101 cggtcagttg atcgagcgtg acgttccccg tcggcaactg gaccgcttcg gccgaggaga
     5161 gccgtacggc gtcacgaatt ttcgactccg gaggccggta tccctcgcgc ggctccacca
     5221 ggcaccaacc gtaacgcggg gaggctgccc agatctcggc ccagtcgtct tccgtgaacg
     5281 tgtcaaggca catcggcagg aggatattgt cctccttgta gatcatctcc tcgacggctg
     5341 ccgcgaggct ctccccggcc gtgtccgcca ccagtttcca ttcgtccgca ccagctcctc
     5401 gttcggcgag cgccgccccc aggcccttga gcatctccct gacttcgtcg tcttttgccc
     5461 acatcacctt cgaagggccc gagatgtcgt gctgctccag gcaggaaaag atcgtgtgct
     5521 cctttcgctg atagtgcttc tcgatgtcca tcaggtcgtt gtaagcttga cgccagcaca
     5581 acagactggc tcccggatcg gcatcggcgg ccagcgcccg gatctccgcc atcgcctgcc
     5641 gcatcttgag aaggacaccg cgcaacgcct cgttctcccg gcggaacgtg tccaccggat
     5701 gccccggcgg cacagtccgc ggaggcaact gaaccaggac ctcgcgcgtc acctgcgagt
     5761 ggaggtcgca catgcaggtg atctcctcga ccggcatgcc ttcggccatg agctcctgct
     5821 ccatggccac gatctcggag tagtccgttt ccttgacgat ctgccggagg cggtctttca
     5881 cctgctccgg cgcctgtccg gaatgaaggt gcttgatgat gtctttcagg gttcggacac
     5941 gatgcgcccg gttgtcgatc agttcgctca ctcgattctc cttgcccggc cagttctcag
     6001 atatccggac ctgattccac gttagcagcg ggatcgcgcc ctcgaccatg actttagtca
     6061 caccccgtcg tatcatcggg gaatccggcc atgaccccgc tctcaccggc ggatctcgac
     6121 accgccatcg cctttccgtt tcttccattt cgcggcggcc gcctcgatct cgacgcgcat
     6181 cgcctgaatg cggccttcct ggcgcgacac tgctatctgg atggcgggcg acggcgcgtg
     6241 atcgccatcg gcggaagcag cttgctccac catgtgacgc cggctgagca gctcgatgtg
     6301 gtccgcatcc tctgcgaaga ggcaggagag cgtgcacgtg ttatctcggc cgtgctgccg
     6361 acgccgctct gcgaggccga gcgaatcgtg cgcgcgcaga tggccctgcc tcgcccaccg
     6421 gacgcggtca tgttcctgcc gatgatcggc ggttacaatc ccgagggcgt cgggcgcgac
     6481 ctgcaccggt tctgtgaccg gttggggcgg gagaccggca cgcggttcat cctttaccta
     6541 cgcgacgctg ggctgcgcga agtgtactgc cggctcgtac gggaatcgga gtacgtgctg
     6601 ggagtcaaaa tcggcacctc ccacgacgac gtcgctccgg cgcgcgaggc gctcggcggc
     6661 gagggagcgg tcgtctgggg catgggcgac ctctgcactc gtgccgtgcg tctgggcgcc
     6721 cgcggtcaca cgtccggcac atctttgctc agcctgaggg cgagcgacga gatcaacaat
     6781 gcgcagcgcc ggggagactt cgaggctgcg gaagaaatcg aggaggaatt gcgggaatta
     6841 gaggagatcc gcttcatgca gggacggatc tacaactatt ccgccctggc tgaagcgctg
     6901 agaatagcgg cgttcgatga tgtcgaaccg ggtgacggcg gacctttcaa tgctccaccg
     6961 ccgccggaga tctgcgccag gctggagcgg atcgtcgatc ggctccggcc gtatcacttt
     7021 ccgccacgga gcccatagga acccagtggc gcaggccatc gttttccgtg gccagcgaag
     7081 gcggcagacg acaaaagccg atcgtctgcc ccactgctct agcgtgtgtt cactccggac
     7141 aggcggacga cgcaggcgta ctgcgccttc gcggcgtcgc ccggtggaac aggcatctcg
     7201 acgaccacgt ctttgccggc cctcgaccac ttgaggtcgc catcggcgcc cagtagtttc
     7261 gctctcatcg tggccggagc atcgatgtcc cggaccgtga gctttccacc cggccagccg
     7321 ggcacgatcg cgtaaagact gtcgcctttc acagtgaaga atgcttcgat gacggcctgc
     7381 cccggctttc gttgctcgac gtagtccgtg atctcgtact tcgtcatgaa ttcgccggtt
     7441 tcgagctttg gttgctctcc cgcgctccac tggcgcgtgc gtctccaggg ccgtgtgccg
     7501 tagatcgcct ctccgttcac tttgagccag cctcccatcc gggccagccg ctcctgcatg
     7561 atcaccggaa tccggccgtc cgcggtcggc ccgatatcca gcaggaagtt gccgccgcgg
     7621 ctcacaaggt cgatcagcat gagaatgagt tcgcgcccgg tgcggtaatc gccgagttgt
     7681 tccgcgcggt tgtaaccata agagaaaccc atcccgcggc tttcctccca cgggtgcgaa
     7741 atgtcctgta gccctgccgt gtactcagtc gtatagtaac cgccgtgctg gtgccgcgat
     7801 cccttgcccc atcgatcgtt caccacgaca tcctttcgca cgggcgactc gttgaagagc
     7861 catgccagca gctccggcgc cttccagtcc tcaccgggca tatcccactc gccgtcggag
     7921 aagatcagcg acggctgata gcgcgtaacg aggtccttga actgcggatg catgtgctcg
     7981 gtcacgtagc gcttgcgatc cgagagccac agcggattga accactcgta gagcgagtag
     8041 tagaagcaca tgcgcagccc gcgcttcctc acggcggccg tcaggtcgcc gagcacgtcg
     8101 cgtttcggtc cggtctcgac ggcgttccac gggcgtttcc acaccgcgct cgcgtggctg
     8161 ctcggccaca gagcgaatcc gtcgtggtgt tttgatgtga gtacaacgta ctttgcgccc
     8221 gacttttcga atacttctgc ccagtagtcc ggatcgtaca tctcggcgcg gaacgccggc
     8281 gcgaaatcct gatacggaaa ccgctcgccg taattcttcg cgtgaaactg ccacgtcgga
     8341 ttcggcttgc ccttgatcgt tggtccgttt tgcagcgcgt tccaatacca ctcggcgtac
     8401 tgcccaacgg gcgcgtacga cggcacggaa taaacgcccc agtggatgaa gatgccgaac
     8461 ctcgcgtcgg tgaaccactc cggagtcggg cggctgtcga ccgactccca cgtcgctgcg
     8521 tacttggttt gagaataggc ctcagggttg atgctcacgg ctgctgccaa caagaggacg
     8581 agtttcatgt cttctacata ctatgcgaag ctggtttgcc gagacaggaa ggtccaactc
     8641 atgcgaaatg caactcttat cctgacaatc gtcctcacat tgactctcag catcgcgcac
     8701 gcccaaggcc ccatcacgtc ccccgagaag ttctttggct tccaactcgg cgccgacagg
     8761 aagatcgcgc gctgggacaa gatcgttcag tattacgagc tgctggagaa ggaaagcgcc
     8821 ggcaggctca aggtcgtgaa catggggccg agcacgatgg gcaacccgtt cttactcgtc
     8881 atcatttccg ctgccggcaa tctttcgaag cttgatcggc tgcgggaagt gaacgcatcc
     8941 atcagcgatc cgcgcggcct cacggagcag gaggtccgga agctggtcgg ggaagggaag
     9001 gcggtcgtct gccagaccat gagccttcac gccaccgaaa tcggcggcac ccagatggcg
     9061 ccggagctag cttacgacct tctgtcgcgg agggatgacg agactcggca aatcctcgac
     9121 aacgtgatct tccttatggt gccgtccttc aaccctgacg gcgcgatcat ggtggccgac
     9181 tggtatcaga aaacgctcgg cacggaatac gagggtggga gtctcccctg gctttaccac
     9241 aagtatgccg gccacgataa caaccgtgac gcattccagc agaatctggt cgagtctcaa
     9301 tatgtcgcga agatcatgtt tcgcgactgg gttccgcagg cttacatcga ccatcaccac
     9361 atgggcagtt acggcgcccg catctacgtc ccgccgtacg ccgagccgat gcgccctctg
     9421 tccgatcctt tggtttggcg cgagatgagc tggtacggcg cccacatcgc ttacaaggaa
     9481 gaggaaggtg gctactccgg tgtcctcaac atggcgcagt attccggctg gggccatttc
     9541 ggatttcact ggatcacgcc cttccacaac atcgccggca tgctgaccga atccgccagc
     9601 gcgaagctcg cgacgccgct ctacatccat ccccaccaat tgaagggcga ggtacgcggc
     9661 ttgcccgact acaacgtcca gacgaccttc cccaatccct ggactggcgg atggtggaag
     9721 ttgcgcgaca tcgtggagcg acagaaagtg tccgcatggg cgctgctcga tctggccgcc
     9781 cgcaaccggg agaccgtgct ctggaacgcc tacctcaagg cgaaacggca gactgagcgg
     9841 ggcgctcagg gcaaacccac tgcctacgtc atcccgaaat cccagcatga ttcactcgcc
     9901 gcggtcatgc ttgttaacaa gctgctgctt cagggtatcg aggtgaagca ggcgaagaac
     9961 tcgttcacgt cggcgactgg gatgacctac ccggccggtt cctgggtagt accgctcgcg
    10021 cagcccaaga tgggtgtcat acggtacctc ctgggacgta cccgctatcc cgacaatgac
    10081 tggacgagag cgagggacgg ctcaccgatc cgcccgtacg acatggcggc cgacaccatg
    10141 ttcgagttca tgggcgttcg agtggacccg ttcgaagatg ggctcccggg agaccttccc
    10201 aagctgaccg gcgtgcttca gccctccggg aaagtggagc agaaccggaa cggctactcc
    10261 ttcgacggcc gtctcaacga atcgttcaag gcgctgaact tactcctgga caaaggcgta
    10321 ggggtgaggc gcgttgagaa ggagggcgat ggtctccgcg cgggcgattt tgtcgtgtcg
    10381 ccggggtcgg aggctctctt gaaggaagtt gcccaggcca cgggcgtgga ctttacagcg
    10441 ctgagaggcc ggccggctga gggtactcac gaactgaagc gattgcgcgt cgccatgtac
    10501 cagcgttacc gcggcggcaa cattgacgaa ggctggaccc gcctgctcct cgagcagtac
    10561 cacttcccgt acacgacgct gatggacgcc gaggtcaaga agggcggtct caacgagaag
    10621 tacgacctca tcatcctgcc ggacgattcg accggggcca tcgtcggcga aacgacagaa
    10681 tcctcgcatc gcgacgagcc cttcccgccc gagtaccgga gcgggatcgg taaggaagga
    10741 gtcgacgcac tgaagacatt cgtcgagaaa ggcggaaccc tggtcgcact gggaggcgca
    10801 agcaatttcg ccatcgaaaa actcgggctt cacgtgcgca atgtgaccgc gggcaggagc
    10861 tcaaaagaat tctggtgccc cggctcgaca cttaaggtcc agttcgacaa cacgcatccg
    10921 ctcgcctggg gaatgccggc agaaggcctc tcgctctttg tcggcgggag tccggcgttc
    10981 gagattctgc cgaacgaaca caacgattgg tacgaggtga tcgtccgcta cgccgatcgc
    11041 gacctcctgg agagcggctg gctccttggt gaggagaccc tggcgaagaa ggccggcatg
    11101 atcgcagtga agcaccgcga cggccgggtc atcctcatgg gttcgcgccc gcagcaccgc
    11161 gcccaaaccc acggcacgtt caagctcttg ttcaacgccc tgatccgcta atacgaggcg
    11221 acggctgtca agacctacgc cagcgggttc ttccaatgca gacccgggaa gtgggcgaag
    11281 tcgccgtcac aggagcataa ctccgctccg tgctcaattg ccagcgcggc gaggtgagca
    11341 tccgacgtta ggttaccgcc agttccgagc gagcctaaca attggcgcag tatcgtcagg
    11401 tgcctgggac ccggatgtac gacagttacc gttggctggt cgagccatgc ggcaaccaga
    11461 tccagagcat gctccggcga caacggattc cggaacacgc cgtgccgggt cgtcaaccgc
    11521 agaaaggcca gcagcacgct ccaacagacg gcgaccgtat ccggtcctga gatggcgccc
    11581 tcgatccacg tcttcgcttt tgaatgcagg ggagcgtctt tgtttaccgc gtagaggagc
    11641 aggttggcat ccagcagtat catttccgaa gcgacagctt gcggctgagg tcctcatcct
    11701 cgagagcttc ggccgctgca agagctttgt cccaacggaa attctgctcc ggtcccaagg
    11761 aataggtttt ctgtgcgaaa cgccggccgc ggctggttcg cgcgcttgac aggccgactc
    11821 gaacggcctg gtttagcgct tgcttgaaag agagccctct cgcacgcatg gcagcacgga
    11881 ccaagcgctc gacatccgga tcaagggtga ccgtggtccg catgctgaca tcatagcatc
    11941 aaaagcgatg ctgtcatgat gcctgctatt cctccgatcg gaacggcgat gccggcagcc
    12001 ccgcggcgct gtaaaggtta gagcccggat cgtccgacca cccgtagcgc gcgagccgag
    12061 gttccgcgac ggaggaattc gatacgatca ccgtcgagcc gtcgatgcgc gcttccgcgg
    12121 caacaaactt gtgatcggcg cctgccacct cgaagccccg caaggagccg ccttttgccg
    12181 caagtccacc ggcgtggtcg aaccaaaccc gaatcgcctt gccctcgcgc gtcgcctggc
    12241 gaaacagcgg gcccgagtac tcgacccgct cgccgtactc gatcgcacgg gcggccagtg
    12301 caagcctcgc gcccacatcc tgcttgttct ttgggtggat atccgccggg tcgccgatgt
    12361 cgatcgtgac cgccatgccg gtcttcgcga gcgccaatgt ctggcgctgc gcctcgcgca
    12421 cctcaggcca cttcgtgtcg ggaccgggcc tgtagttggc aagctgcacg aagaggaacg
    12481 ggaagtcgcc ctgcccccat gcgcgccgcc agtcctgaat catcgttcgg aacaggcgtg
    12541 cgtacaaggg tgcacgcgcc gccggagcgt tgctctcgcc ctggtaccag atggcgccac
    12601 ggatcgggta ccgcgtgagc ggagcgatca tcgcgttgaa caggcccgcc ggcgcccaac
    12661 agccgcgctc gttcggctcc catggaaatc cgggcggctc cttccctgcg gccttcgcct
    12721 tctcgacggc ctgccgccac tcatcaagct gcttctctcg ccgcaggctc gaaacggcga
    12781 agtcgtcgtt catgcgcgac cactcggcga ataccggcat cagcgacgca tcggcggaga
    12841 tggagcgcag actggtccac gcgtccgccg gcgtgccgcc ccacgccgtc tcgatcaggc
    12901 cgatcggcac ggcgagttta tcctcaaggt ttcgaccgaa gaaatacgcc acggccgaaa
    12961 agtcacgggc cgtttccggc gtgcacggag tccacggctc ggcttccgca tcgaggagcg
    13021 gatagctggc gacgttgttc ttcacccgat agaggcgaat ccgcggatga gtcgccgcgg
    13081 caatttcgtt gtcggcgttc gccgatgcgc tgacaggcaa ctccatgttc gactgtccgg
    13141 aagccaccca cacatcgccc acgagcacgt ctttgagcga gattgtgttc ttccccttca
    13201 ccgtcagttc aaagggtccg ccggcttcgc gcggcggcag atagacgctc cagaagccaa
    13261 gcgaatccgc cgtggtcgaa gtcgagtcgt cgccgagagt gacattcacc gcctcgcccg
    13321 gcgctgcttt cccccagata tggaccggca gtccgcgctg gatgaccatg tggtcggcca
    13381 gcagcgtagg cagggaaacg tccgcgctcg cggatgaact cagtgcaact gcacaaaaca
    13441 ggatcgacaa aaggaatttc tgtatcggca tcgaccacca atcatacagc ccccatcccg
    13501 cacgctgtat gatgaacact ccctcgggag aaccacgcat ggcgagcgct ccgtttggaa
    13561 atgatccggc gaagatcggg cggtacaggg ccttctggaa gagggacgat gtccggcgtc
    13621 ccctcgtcgg cttctctctc gtgggatggt ttccgctgca ggaattcgcg gcttgcagag
    13681 cttggaagaa taccgagtat ctcacgctcg acatgatcga ccccgaggcg ttcctcgacg
    13741 accacctgcg catgctgcgt gagggcgaga tcttggacga tgacgtgatc cgcggggcgt
    13801 gcccaggcca agtggcgctc ccttggcttc ccgcaatgct gggctgcaag atgcgtattc
    13861 ttccgcacaa cgtgatgggc gaggaacagc acctcccctg ggaggaagct ctggcgattc
    13921 gtctggacct agacaacccc tggttccgga agtaccagga gttcgcggat gttcttgtgc
    13981 gcgcctcgaa cggagcgttc cccgtcagtc attccgccga aatcggtccg agcgatctgc
    14041 acgcaaccct gcgcggccac accaccggcg tcctggacct ggcggacgaa cctgaaaact
    14101 cagcttgcct tttgtggcga atgggcgaaa tcttccggca ggtcacggaa gagttctgga
    14161 aacgagttcc cctcttccag ggcggctact tcgatgccca atactcgcta tgggcgcctg
    14221 ggccgatcat ccgcatgcag gaggacgcct ccgcagtcta ctcgccggcg ctgtaccgga
    14281 agttcgtaca gccggtcgat cggatgctcg cggcgcattt cccgagcagt ttcatccacc
    14341 tgcactccac gtcgatgttc ctgctcgatg ccttcctcga gatcgcggag atccaatgct
    14401 tcgaggtgaa taacgacgcg cttggcccgc cggtggcggc gatggttccc catttctgca
    14461 agatccagag ggcaggaaag ccgctgctca tccgtggctc cttcaccacc gaggagatgc
    14521 gcctgctcat ggactcgctc gagccgcgtg gcctcttcct ctacatcatg gtgaacgaga
    14581 tgcgcgagat cgacgagcta cggctcctcg ttgggttgta gtcctacttt tccccctgtc
    14641 cgcgccggaa tccgcgataa tacaacgatt ggggttaagg aaccgcggcc gtcagggccc
    14701 ggcggtctca aaatgtcaga caccgatggc attcttctcg gaactgatgg caggccgcgt
    14761 tgcttctggt ccgcctccgc tccggactac gcgcggtatc acgacgacga gtggggctat
    14821 cccgtcaccg atgaccgcag gctcttcgaa aagatctgtc tcgaaggctt tcagtccggc
    14881 ttgagctggc tcactattct tagaaagcgc caggacttcc ggcgggtctt ccacgatttt
    14941 gacttcgaac gggtcgcgcg attctcctcc cgcgacgtgg agcggctttt gaaggacgct
    15001 tccatcgtcc gtcatcgcgg caagattctg gcggcgatca acaacgcacg cagagcccgt
    15061 gatctcgccc gggaattcgg ctctctgtcg gcctacttct ggacgtatga gccggcgccc
    15121 tcgacgcgtc ccggcctgct cacctgggag gtgctccgcg gcatgacaac cagccccgag
    15181 gccacggcgc tgtccaagga tctgaagaag cgcggctggt cgtttgtggg tccgactacc
    15241 gtctacgcct tcatgcaggc gatgggcctc gtcaacgacc acctgaacgg ctgttttgtg
    15301 cgtcccctcg ccgaagccgc ccgccggaag tcgggccgta tcgtggtcag atcttcggac
    15361 ccggtcccgg cccctccggg aagaaccgcg cgagatcggc gtcgctgaac ggcgcacgta
    15421 cgaagcggtc cttcatccgg aaaggcacgg tgcaatcgaa gatcgtcttg caggagatgc
    15481 cctctcccgc gatcgacggg ctgaactccg gcgactgcga cggatcgagc ggatggcatc
    15541 gcactcccgg gataaacgcc gtgctcgcgt ctccctggta gcgcgtcgtc atcgcccaga
    15601 gcacgtcgtt ggtgtcgtag atgtcgacgt cctcgtccac caggatgacg tgcttcagct
    15661 cggggaacgc cgagaacgcc gccagtgccg cctgccgctg ccggccctcg tcggacggcg
    15721 ccgccttgcg gaattgcagt atggccaagt actttccacc gccggccggg tgcgcgtacg
    15781 cgttgagcag ccgccccggc atcgagtcct cgacgaagcg caggatgctt gcctcggtcg
    15841 gtatcccgac aaggttcgtg tgttccgcgc cgggactgac gagcgtctgc aggatcggct
    15901 gccgccggtg cgtcactgct ctcacccgga tcaccgggac cgacatctgc gcccgcccca
    15961 gatatcccgg aaactcgccc atcgcccatc cgccgcccgt tcgctcgtcc tcccggacgc
    16021 gctcgcccgg caggatttcg ccctcgatca cgatttcggc gcgcgccacg gccttcgcct
    16081 tcaccgacac gcaggacgca agctccaccg gccgcccgcg caacgccccg gccacgctca
    16141 gttcgtcgaa gcccagcgga gtggtcggcg gctcgaagca cgcggcgacg tagaccgccg
    16201 gatccagccc gatgctcact gataccggca gcgactctcc gcgggcttca gctttcctgt
    16261 agaactcgcc gatgtggcgg cccggcacga agtagacgct cagccggtcc ggccccagga
    16321 cgcacatccg gtggatagtc acgtccgact caccggtctc gggatcctcg ccgcgcagca
    16381 ggccgagcgt gaagtacggc ccggcgtcct tagctgtccc agtgggcact ggaagcagcc
    16441 gtgtcagatc gacgggcgcc cggtggatca cctcctggca cggtgcggtc gagccttcaa
    16501 cgaccgtcgc aggaactgga cgctccaccg cctccatcaa tcggaacggc agacggtcca
    16561 ctgtcgtgtc gagtagcaac gcagttcgct cgcggctgcc gaacaccccg accaggatgc
    16621 gcgcctcggg atatccccgc acgttttcga acagcatcgc cggcccgatg cgggttggcg
    16681 gcgcgatcgg cgtgccagcg ccggccagcc ggtacacccc ggccagttcc agataggggt
    16741 ccaccggctc ctgcgtctta tggagatgtt gcgggaaacc tgccagaaat gtcagtgcgg
    16801 agcgaagatc gcggaccccg ttcgccccgt gggaaggatg ctgtgtgctc attgaacaca
    16861 ccagtatcgc ccgcgagcga catggatgta agtcgttttc gggtgcccgc caagacccag
    16921 caatgtctgg gcgcaccaac tccgtcactt ctgcaaacgc gtctcgggcc aacttccgag
    16981 tcggcgacca tacacccggg gtcggtaaac gaagcggcgt caggcgcggg taagccggat
    17041 caacttcccg gaccgggact tcttgggcgt ttttggatcg tcctcaccgc aaccct
//
LOCUS       DATIFQ010000005         3523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1533986, whole genome shotgun sequence.
ACCESSION   DATIFQ010000005 DATIFQ010000000
VERSION     DATIFQ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533986"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VLE22_00295"
     CDS             complement(<1..495)
                     /locus_tag="VLE22_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB12861.1"
                     /translation="MKDLNLSRRHFMAGSLAVSAAGCSRVLGANDRLRVANVGCGRRG
                     LLKELIELKDSANIEIAAVCDTWRQKREKAAADVKEFTGKEPLQTAHFTEVLGKDIDV
                     VTIGTPDHLHCSLLIEAIKAGKDVYVEKPLAMNMRELIRAYDTVKQSDRIVQIGTQMR
                     SYANS"
     gene            693..1898
                     /locus_tag="VLE22_00300"
     CDS             693..1898
                     /locus_tag="VLE22_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB12862.1"
                     /translation="MIRIGMIGCGGRNTEAGGQALTADKGNRLVAMADIALDRVQERR
                     SELKVKFPDQVDVDDAHCFTGFDGYKHVIESSDVVLIANAAKFHPLHMMAAIEAGKHV
                     FVEKPHAIDPAGIKMVRAACELARKKKLSVLSGLQSRYTPMYREAIERVHAGAIGDIV
                     SIEERYYRAPYVLRARKPGQTEVEHQGSNQYHFHWLCGDDIPQSLVHNLDRANWAMKG
                     EPPVRCIGMGGRSTLNEERYGNVFDHHAVIYEYANGVRIHAFCRTIPNCFNQTSSMLV
                     GTKGRCFTMQGRIEGENPWQYAGPKMYSGVHTNPYQIEHDELFKAIRAGDPLNCGDYM
                     AQSTLIGIMGQISCYSGKDVTWEKVMTSDFYFAPRPEEVTKDMEPPTRPGPDGVYPVC
                     FTPGVSKLL"
     gene            2035..2397
                     /locus_tag="VLE22_00305"
     CDS             2035..2397
                     /locus_tag="VLE22_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12863.1"
                     /translation="MRNRRRLCVLMAAIGLALALVAAAADVSGKWRAEFTTPDGTQRV
                     NTFTFKVDGDKVTGTVAGSQDETPIKDGQVSGDVISFTADRPFGTFTYKGKISGDEIK
                     FAVQFNDNNFEITAKRLQ"
     gene            2456..3028
                     /locus_tag="VLE22_00310"
     CDS             2456..3028
                     /locus_tag="VLE22_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12864.1"
                     /translation="MFTDRLPAGESLRVEYFKSDRLHTLDVPEDDRLHAPATRIQAAM
                     ESGESRAVRKPCQEFLFASADFYGVVRPQIRVLAARPLRVREGGWATELFGDYHREKK
                     LIRVWMRTAVRRQVTSFGTFLSTLCHEFCHHLDMERLGFRDSPHTRGFYGRAAVLYHH
                     ARGTPPKGLFWLPMRDGRWRIDWPRTNRGA"
     gene            3229..>3523
                     /locus_tag="VLE22_00315"
     CDS             3229..>3523
                     /locus_tag="VLE22_00315"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB12865.1"
                     /translation="MLGILERFSATAVRDPERRANGRFPMVRDVHYKVIHSKGRDEIG
                     TGQTVNISSAGVLFTAQSPLPPGRRLEVSISWPAQLDGKCGLKLIARCRVVRCT"
BASE COUNT          663 a         1074 c         1130 g          656 t
ORIGIN      
        1 cgagttcgca tagctgcgca tctgcgtgcc gatctgcacg atgcggtcgg attgcttcac
       61 cgtgtcatag gcacggatca gctcccgcat gttcatcgcc agcggtttct ctacgtagac
      121 atccttccct gctttgatgg cctcgatcag caggctgcaa tgcaggtggt cgggcgtgcc
      181 gatggtcacg acgtcgatgt ccttccccag cacttcggtg aagtgcgcgg tctggagggg
      241 ctccttgccg gtgaactcct tgacatcggc cgccgccttc tcacgtttct gccgccacgt
      301 atcgcatacg gcggcgatct cgatattggc gctgtccttc agctcgatca gctccttcag
      361 caggccgcgc ctcccgcagc ccacattggc cacgcgcaag cggtcgttgg cgccaagcac
      421 tcgggaacag ccggccgccg aaacggccag cgaaccggcc atgaaatggc ggcggctcag
      481 gttcaaatct ttcatccgtc catccctccg gatggattgt aacctggagg gcctgtggct
      541 gcgttaaacg agtagtccct ggattacact agccaactgg agggcccaca ccgatggacc
      601 aggtaaatcg atctgaaggc agctcgcgcc gggatttcct caaggcctcg gccttagccg
      661 ttccggcgat ggtacatgcc gcgggaagcg atatgattcg catcggaatg atcggctgcg
      721 gcggccggaa tacagaagcg ggcggccagg cgctgactgc cgataagggg aaccggctcg
      781 tcgccatggc cgatatcgct ttggaccgcg tccaggagcg gcgctcggaa ttgaaggtga
      841 agttcccgga ccaggtggac gtggacgacg cccactgctt caccggcttt gacggttaca
      901 agcacgtcat agagagctcc gacgtggtgc tcatcgccaa cgcggcaaag ttccatcccc
      961 tgcacatgat ggctgccatc gaggccggca agcacgtctt tgtggagaag ccccacgcca
     1021 tcgaccccgc cgggatcaag atggtgcggg ccgcatgcga actggcgcgg aagaagaagc
     1081 tgtcggtgct ctccgggctc cagagccgct acacccccat gtaccgggag gccatcgagc
     1141 gggtgcacgc cggcgccatc ggcgatatcg tatccatcga agagcggtac taccgcgcgc
     1201 cctacgtgct ccgcgcccgc aagccgggac agaccgaagt cgagcaccag ggctcgaacc
     1261 agtaccactt ccactggctc tgcggcgacg atatcccgca gtcgctggtc cataacctcg
     1321 atcgcgcgaa ttgggccatg aagggcgagc cgccggtgcg gtgcatcggc atgggcggcc
     1381 gctccacgct gaacgaggag cgctacggga acgtgttcga ccaccacgcc gtgatctacg
     1441 agtacgccaa cggcgtgcgc atccacgcgt tctgccgcac catcccgaac tgcttcaacc
     1501 agacctccag tatgctggtg ggcaccaagg gccgctgctt caccatgcag gggcgcatcg
     1561 aaggcgagaa cccgtggcag tacgccggac cgaagatgta ttccggtgtg cacacgaacc
     1621 cctaccagat cgagcacgac gagctgttca aggcgatccg cgccggcgat ccgctcaact
     1681 gcggcgacta catggcgcag agcaccctga tcgggatcat gggccagatc agttgctaca
     1741 gcggtaagga cgtcacctgg gagaaggtga tgacatccga cttctacttt gcgccgcggc
     1801 ccgaagaggt caccaaggac atggaaccgc ctacccgtcc cggtccggac ggcgtctacc
     1861 cggtgtgctt cacgccgggc gtcagcaagc tgctctaaga aacggggact gacaccattt
     1921 cgccgctttt ggctcgggac aggcctactc tgccctgagc gaagtttgcg agcgggcaga
     1981 gctgcctgtc ccatctcccc gtcgtagtat cgttgtccac agggggtgcg agtcatgaga
     2041 aatcggcggc ggctgtgcgt cttgatggct gcgattgggt tggcgctggc ccttgtggct
     2101 gcggcggcgg atgtaagtgg caaatggcgg gcggagttca ctactccgga cggcacgcag
     2161 cgcgtgaaca ccttcacgtt caaagtggac ggtgacaagg taacgggcac ggtagccggc
     2221 tcccaggatg agacgccgat caaagatggc caggtgagcg gagacgtgat ctcctttacc
     2281 gcggaccggc cgttcggcac gttcacctac aagggcaaga tcagcggcga cgagatcaag
     2341 ttcgccgtgc agttcaacga caacaacttc gagattacgg cgaagcgtct gcagtaggta
     2401 cgtgggttcg gggtttggca agggctagaa tgggttcgtt gggagattgg ttgcggtgtt
     2461 cacggaccgc cttccggccg gcgagtcgct ccgggtggag tacttcaaaa gcgaccgcct
     2521 acatactctt gacgtgcctg aggatgaccg cctccacgcg ccggcaacgc gcattcaggc
     2581 agccatggag tccggagagt cccgtgccgt gcggaagccg tgccaggagt tcctgttcgc
     2641 gtcggccgat ttctatggcg tcgtgaggcc gcagattcgg gtcctcgccg cgcgcccgct
     2701 tcgagtgcgc gagggcggct gggcgaccga acttttcggg gattaccatc gggagaagaa
     2761 actgatccga gtctggatgc ggacggccgt ccgcaggcag gttacctcct ttgggacgtt
     2821 tctctcgacg ctctgccacg agttttgcca ccacctcgac atggagcggc tcggtttccg
     2881 ggactcgccg catacgcgcg gcttctacgg ccgcgccgcc gtgctctatc accacgcgcg
     2941 gggaacgccg ccgaaggggc tcttctggct gccgatgcgg gatggccgct ggcggatcga
     3001 ctggccgcgc acgaatcgcg gcgcgtaatt cttgcgtcgc agtaatccct ccacttcgtc
     3061 tgtccccgct tttcccgaat ggacgcacct cagtgggcat cggctagcac tggcgtgaca
     3121 ccgggggagt acggcagcac tatctggtgc accatcacaa atgaacctat ttctctattg
     3181 ccgatgcgag cgggaaagcg gaggatgaac aggaaagagg atctacggat gttaggaata
     3241 ttggagcggt tttcagcgac agcggtgcgc gacccggagc ggagggcgaa cggccgtttt
     3301 ccgatggtgc gtgacgtcca ctacaaggtg atccattcga agggcaggga cgaaatcggg
     3361 accggccaga cggtcaacat cagcagtgct ggagttttgt ttacggcaca gtcgcctctg
     3421 ccgccaggca ggcggctgga ggtctccatt agttggccgg cccaattgga tggcaagtgc
     3481 ggtctgaaac tgatcgcccg ttgcagggtc gtgcgctgca cgg
//
LOCUS       DATIFQ010000006         3402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_959657, whole genome shotgun sequence.
ACCESSION   DATIFQ010000006 DATIFQ010000000
VERSION     DATIFQ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959657"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VLE22_00320"
     CDS             complement(<1..793)
                     /locus_tag="VLE22_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HSB12866.1"
                     /translation="MRCRVGVLAFSFSAILLAQSPVPSGFEAWPVPIEKRVFAGSFSR
                     SPGERAIKLAGCRNEFLSAQLAVRNSKESELSYQVEPLRGPEAAAIPAEEIRVRFGAY
                     LPVDETGQYTADPLLESKSIRLPANLAQPVWLTIHVPKGIAAGKYAGSLRLAAGGESL
                     DVPLEVEVFSATLPDPPDYSYYLNLWQDPNGVARAHKVDLWSEAHWQLLARYAENLAM
                     HGEKSIMTSIIHDPWNAQTGYIFPTMVEWKHPGEWKQGEAPKFAWD"
     gene            complement(1079..1963)
                     /locus_tag="VLE22_00325"
     CDS             complement(1079..1963)
                     /locus_tag="VLE22_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSB12867.1"
                     /translation="MDLALVIRRRLGKLALEQRGLAAAAHVTESYISQLLSGKKAPPA
                     PNRTDVYEKMELFLGFPNGELAKLARLQRQEHLKKRVEAPPRPLFKDFRDLILRKCNP
                     EKGRQVRAIFEKEPFGELERLVTQKLLDVAKGVAKEELASEKWLRMVARLSKRSYEQT
                     RVMILEFLDTDVFHVSIDNCVSFMDPLIESWDIDLATFGMEIVLNRRLTLGHLKRFDF
                     VEREPERPFDVEPGFEEFLEDAALSGDVRDEEIEFLKLLRFKDKRPTPLYYYRELQNL
                     RDPLHFRASAQKTQPDST"
     gene            2162..2401
                     /locus_tag="VLE22_00330"
     CDS             2162..2401
                     /locus_tag="VLE22_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12868.1"
                     /translation="MFDPSRKSTGTGYTMPVAWLAVAFVFIPAVLAASHPFGYASLSL
                     AVVCSVLCVMIAWISWKKSSQLSIPSIAVRESKAK"
     gene            2434..2661
                     /locus_tag="VLE22_00335"
     CDS             2434..2661
                     /locus_tag="VLE22_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12869.1"
                     /translation="MTLLTLKLTVEELEVLTTLAADQLFRTEFIDPKFPGYERNSAKL
                     SLGKALVGRLRSIIDPASATRTPSARMIPTA"
     gene            2759..>3402
                     /locus_tag="VLE22_00340"
     CDS             2759..>3402
                     /locus_tag="VLE22_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12870.1"
                     /translation="MPREIRRRDFVRGSAGLLSAGVALAPPNAEAAQKRTTGAASQRL
                     YKSKWYEFTDHPESDTCWSLSVGPDGRIYAAACAEGVPGGVVKVVRYNEQKDALDLLF
                     SLDEKVNDPPESGRATQCKIHYSFVPSMHDGIMYMATHLSGPPIDQPMYSPWRSWHDK
                     ERCFRGAALLAFDTKTDEVKWWDTLAPKEGCRCLLHDEERGLLYCATYPRDHFI"
BASE COUNT          687 a         1068 c          956 g          691 t
ORIGIN      
        1 agtcccacgc gaacttcggc gcctcgccct gcttccactc gccggggtgt ttccactcga
       61 ccatcgtcgg gaaaatgtaa ccggtctgcg cgttccaagg gtcgtggatg atggacgtca
      121 tgatggattt ctcgccgtgc atcgccaggt tttcggcgta gcgcgcaagc agttgccagt
      181 gcgcctccga ccagaggtcc actttgtgcg cgcgggcgac gccattcggg tcctgccaga
      241 ggttgagata gtaggaatag tccggcgggt ccggcagagt cgcgctgaag acctccactt
      301 cgagcggcac atccaaagac tccccgcccg cggcaagtct caggctcccg gcgtacttgc
      361 ccgccgcgat tcccttcggc acgtggatcg tgagccagac aggttgcgcg agattggccg
      421 gaagccggat ggatttcgac tccaagagcg gatcggccgt gtactgtccg gtttcgtcga
      481 cgggaagata cgccccgaag cgcacccgga tctcctcggc ggggattgcc gccgcctccg
      541 gaccgcgcag cggttcgacc tgatagctca gttcggattc cttgctgttg cgcacggcaa
      601 gctgcgcgga cagaaactcg tttcggcagc cggcgagttt gatcgcccgc tccccggggc
      661 ttctcgaaaa gcttccggcg aagacccgct tctcaatcgg aaccggccag gcctcgaagc
      721 cggaaggaac gggcgactgc gccaacagaa tcgcggagaa agaaaacgca agaacaccga
      781 ctcggcatct cataagcagc taccatccta accacaattt ccttttggct cgcggacggc
      841 cggaggcagg ccgtgctgaa gcatcacaag tcttcagcaa gccagcggcg tggaaaccat
      901 ctctcccact ttaacaacgg atgtacaccc cgttggcgtt tttgtcaaag tctcccggaa
      961 acctaacagc gatcactttt cgcacctggc tggtgtaccc tggttcgacg atcttcgact
     1021 cgccgggtcc tgctccgccg ggcgcagatc gacccgaaaa tgagagacgc ctaccgtcct
     1081 atgtggagtc cggttgcgtc ttctgcgcgg aagcgcggaa gtgaagaggg tccctcagat
     1141 tctggagttc ccggtagtaa tagagtgggg tgggccgctt gtccttgaac cgcagtagct
     1201 tcaagaattc gatttcttca tccctgacat cgccgctaag cgcagcatcc tcgaggaact
     1261 cctcgaaccc gggctctacg tcgaacggac gttcaggttc acgttcgacg aaatcaaacc
     1321 tcttcaggtg cccgagtgtc agcctgcgat tgaggacaat ctccatacca aaggtcgcaa
     1381 ggtcaatatc ccaggactcg atcaacgggt ccatgaaaga tacgcagttg tcaatggaca
     1441 cgtggaagac gtccgtgtcc agaaactcca ggatcatgac ccgcgtctgc tcatagcttc
     1501 gcttgctgag ccgggccacc atgcgcagcc atttttcact tgccagttct tccttcgcga
     1561 cgcccttcgc cacatccaga agcttttgag tcacgagccg ctcgagttcg ccgaacggtt
     1621 ccttctcgaa aatggcgcgt acttgcctgc ccttctcggg gttgcatttt cgaagaatca
     1681 gatctcgaaa atccttgaat agaggtcgag gcggggcctc cacccgcttc ttcaggtgtt
     1741 cctggcgctg gaggcgcgcc agcttcgcga gttcaccgtt cgggaacccc aggaacagct
     1801 ccattttctc gtatacgtct gtccgattgg gagcgggcgg tgcctttttc cccgaaagca
     1861 actgagagat gtaagattcc gtcacgtggg cggcagccgc caggcctctt tgttcgagcg
     1921 ccagtttccc cagcctgcgc ctgatcacga gagcaagatc cattgccgct ccattgacag
     1981 aactttacca caagcgctta agcactgcgc tccagtgtcc catggttcgc ttgacaagta
     2041 aagaaacttg accgatactg gttaaggagt gggctttccc acctgctctg cggatcatcc
     2101 gagctaagtc ggctagtccg ttctccgcac cttagccaac aggacaaccg aggaggttga
     2161 catgtttgat cccagccgga aatccacggg aaccgggtac accatgccgg tcgcctggct
     2221 tgccgtagcc tttgtattca ttccagcggt tttggccgcc tcgcacccgt ttgggtatgc
     2281 gtctctttca cttgcggttg tgtgcagcgt gctatgcgtc atgatcgcct ggatcagttg
     2341 gaagaaatca tcgcaactgt cgatcccctc gatcgccgtc cgggagtcga aagcgaagtg
     2401 accggacact ctcgatttct gagccgcgat cgaatgacac tgctcacgct gaagctcacg
     2461 gtcgaagaac tcgaagtgtt aaccactctt gcggcggatc agttgttccg caccgagttc
     2521 atcgacccca agttccccgg ctacgagcgc aactccgcga agctgagcct cggaaaggcg
     2581 ttggtgggac gcctgcgatc gataattgac cctgccagtg cgacgagaac gccatccgcg
     2641 aggatgatcc cgaccgcgta agaagcctcg ggggcgtaac ggaatcgccg tcgcccgccc
     2701 tcctctcgcc aatcgcatcc gattgtgata atttcgatca cggtcaggag attcacgaat
     2761 gccgagagaa atcaggcgaa gggatttcgt ccgggggagc gccggcctgc tatcagccgg
     2821 agtcgcgctc gcgccgccga atgccgaagc cgcccagaaa agaactacgg gcgccgccag
     2881 tcagcgcctt tataaatcga agtggtacga gttcaccgat catccggaat cagacacctg
     2941 ctggtcgctg tccgtcggac ccgacggccg catctatgcc gccgcctgtg cggaaggcgt
     3001 acccgggggc gtggtaaagg tcgtccgcta taacgagcag aaggacgcgc tcgaccttct
     3061 cttctcgctc gacgagaagg tcaacgaccc gcccgaatcc ggccgcgcca cccagtgcaa
     3121 gattcattac agcttcgtgc cctccatgca cgacggcatc atgtacatgg ctacccacct
     3181 gtcagggccg cccatcgacc agccgatgta ctccccctgg cgttcctggc acgacaagga
     3241 gcgctgcttc cgcggcgccg cgctgctcgc cttcgacacc aaaaccgacg aggtcaaatg
     3301 gtgggacacg ctcgcgccga aggaaggctg ccgctgcctg cttcacgatg aagagcgcgg
     3361 gcttctctac tgcgcaacct acccgcgcga ccatttcatc at
//
LOCUS       DATIFQ010000007         5507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1534405, whole genome shotgun sequence.
ACCESSION   DATIFQ010000007 DATIFQ010000000
VERSION     DATIFQ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534405"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VLE22_00345"
     CDS             complement(<1..575)
                     /locus_tag="VLE22_00345"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB12871.1"
                     /translation="MLWALIVAGLLLAVISMRGERRRARYYSAHFAALGRQTRLPRAT
                     VIVPVKGPEDGLEQNLAALASLDYPDYELIVVANLVEDIPRGVVPEHARVVLARGRGG
                     GGSEKIRNLMAAVKAARPTSAIFAFADSDGRPGRLWLRSLVEVLQDERAGAATGYRWH
                     VPERGGLWAILRSVWNAVIAGRFGPGPLRFA"
     gene            650..2518
                     /locus_tag="VLE22_00350"
     CDS             650..2518
                     /locus_tag="VLE22_00350"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HSB12872.1"
                     /translation="MVRLRRHNVGYVVLLASAFVLAMIAGYTALGRQIDADAYDWMFR
                     LRPQPPPATESALLAIDDASLKELGGIWKIRAMLAEGLERISRVSPKVVAIDLTLTEA
                     GEPGDDRRLEAAMRNTPGLVLAAEMLPGGGWQEPWEAFRRTAAAVGHVHAAPDELDGV
                     NREIPLEKIAGRTRHWALSLEAYRLSRGGLRIVESPGELSVGDMVIPAAREDSRSIFI
                     RYLPSGPDGAPGIPRVSVIDLLRDSSAAERLRGRTVFVGVTAQSAARDRLMTPVASLL
                     PMPGVEIHAHAFETLRQGRFLTSAPNLSVVLACALLVGGAGAIFAFVAGWPAYVLGAA
                     LLLAAHAAPFLLFSADTVFPFAAPVSAAWLSVVGAASYQHFVVRRRLEKTEAERARYQ
                     QAVHFVTHEMRTPLTAIQGSSELMTRYSLTEDKRKQIAGLIHSESRRLAKMVETFLNV
                     ERLSAGQMELRREPFSAPEIVAACAERARPLAEKKQIRLRVGEVVDQTLIGDRELMEY
                     AVYNLINNAIKYSPSETEVTVTGRRQSGLFRLTVRDQGIGMDENEIKNLFQKFYRTRR
                     AIASGESGTGIGLSIVEQIVTHHGGRIEVESEPGKGSRFTLVLPATVTAGTGGREV"
     gene            2515..3849
                     /locus_tag="VLE22_00355"
     CDS             2515..3849
                     /locus_tag="VLE22_00355"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB12873.1"
                     /translation="MSDNTNAARLLVVEDDAGVRTTVVTFLELEGYQVDAVSSTREAI
                     RRLNETAYPIVISDIYLDEKTGLDVLDAARAKDPTCAVILMTARGTMETVMAATRGGA
                     FDYIAKPFELDALLATVKRAEASRAGGVDEDESEIDDLPESEMIGSSAQMVAIYKIVS
                     RVAPTDATVLIEGETGTGKELIARMIHTNSPRVPQPFVAVDCASVAPALLESELFGAM
                     RGAYTGADRDRMGVFEAANHGTVFLDEIGDIELNFQLKMLRFLQEREIRPLGSPRSRP
                     VDVRVIAATNRDLQAMVDEGKFREDLWFRVNVVRIHVPPLRERRGDVPLLAHFFVRKY
                     NARYSQSARLTDSALRAMDDYVWPGNVRQLQHMMERLTILAPGGRIDQQAIQESIRAT
                     EPHEHGGETLAEAEMDQIRRVLSATGGNKSRAARILGIERKTLYRKLERVGE"
     gene            4005..4151
                     /locus_tag="VLE22_00360"
     CDS             4005..4151
                     /locus_tag="VLE22_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12874.1"
                     /translation="MFDSLDDQMKHDAEQETTRGQRWLLVLTVVVVSLLVFGGLYFGV
                     RMLE"
     gene            complement(4160..4657)
                     /locus_tag="VLE22_00365"
     CDS             complement(4160..4657)
                     /locus_tag="VLE22_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12875.1"
                     /translation="MKTTLSRTAVMVAGLLATNVTLSPGQHFQPPTYDYDPRLLVLKR
                     FFEDRGSPIDHLAKDFLLAADHYGLDWRLLPSISVIESGGGKEFTNNNIFGWDNGKAH
                     FPSIQAGIHLVARRLAISRIYKNKDLDEKLSVYNSNAEYPALVKSVMARLASDEPPQP
                     GASRL"
     gene            complement(4879..>5507)
                     /locus_tag="VLE22_00370"
     CDS             complement(4879..>5507)
                     /locus_tag="VLE22_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12876.1"
                     /translation="EEGESHVENGAGFRIEARSLQIRPERNAYVALNGANRVLVAASS
                     APVRVTTAEGVLLASLAPGTSLEFEPQAAGAVAPFTVTGCLVKTDGRFLLTDRLANVT
                     LEVRGPNLNDHAGFQVELTGAGLKGVQPAPGATEVMQAAKVRRLHGSCATAALPPAAG
                     SRSRKPGGNAAMSRSTKAIIAGVVIAGAGAGAAVAVSAGEEQKKPISR"
BASE COUNT         1012 a         1759 c         1751 g          985 t
ORIGIN      
        1 cacgcgaacc tcagtggtcc gggaccgaat cggccggcaa tcaccgcgtt ccatacgctg
       61 cgcaggatcg cccacaatcc gccccgctcc ggcacgtgcc agcgatagcc ggtcgcggcg
      121 ccggcgcgct catcctgcag cacttccact agcgagcgca gccacaagcg tcccggccgt
      181 ccgtccgagt cggcgaatgc gaagatcgcg gacgtcggcc gggcggcctt caccgcggcc
      241 atcagattcc tgatcttctc gctgcctccg ccgccacggc cccgcgcgag tacgacgcga
      301 gcgtgctctg gaactacgcc tctcggaatg tcctcaacca gattcgcaac gacgatcagc
      361 tcgtagtcgg gatagtcgag agaggcgagt gccgcaaggt tttgctccaa gccatcttcg
      421 gggcccttca ccgggacgat caccgtcgcc cgcgggagcc tcgtctggcg tcccagcgcg
      481 gcgaagtgtg cggagtaata ccgcgcccgt ctcctctcgc cgcgcatcga aattaccgcc
      541 agaaggagtc ccgcaacgat cagagcccag agcattggaa caaggataac aggagccgcc
      601 ggcgtggttg cgcgtcgggc ggcactccag gtggcacaat agaggatcga tggttcgtct
      661 ccgccggcac aatgtgggct acgtagtatt gctcgcgtcg gcttttgtgc tcgcgatgat
      721 cgcggggtat accgcgctcg gccggcagat cgatgccgac gcctacgact ggatgttccg
      781 tttgcggccg cagccgccgc ccgccaccga atccgccctg ctcgcgatcg acgatgcttc
      841 gctgaaggaa ctgggcggca tatggaagat acgggctatg ctcgccgagg gcttggagcg
      901 gatctcacgc gtctcgccga aagtggtcgc catcgacttg acactgaccg aagccggtga
      961 gcccggcgat gaccggcgct tggaagcagc catgcgaaac acgcccggcc tcgttctcgc
     1021 ggcggagatg ctgcccggcg ggggctggca ggagccctgg gaggccttcc gccgcacagc
     1081 cgccgctgtc ggccacgtcc acgcagcccc tgacgaactc gacggagtca atcgggagat
     1141 tcccctcgag aagatcgccg gccggacccg ccactgggca ctctcgctcg aggcgtaccg
     1201 gttgagccgc ggcgggctcc gcatcgttga gtcccccggc gagttgagcg tcggcgatat
     1261 ggtcatcccc gcggcgcgcg aggattcccg atcgatcttc atccgctatc tcccttccgg
     1321 accggacggc gcccccggca tcccccgtgt ctccgtgata gacctgctcc gggattcctc
     1381 cgctgccgaa cgcctccgcg gccgcacggt gtttgtcggc gtcaccgcgc agtcggcggc
     1441 gcgcgaccgg ttgatgacgc cggtggcttc actcctgccg atgccgggtg tcgaaatcca
     1501 tgcccacgca tttgagaccc tccgccaggg ccggtttcta acctccgcgc caaatctctc
     1561 tgtcgtgctg gcttgcgcgc tgctggtcgg cggcgccggg gcgatcttcg ccttcgtcgc
     1621 cggctggccg gcatacgtcc tcggcgcggc gctgcttttg gcggcccacg cggccccttt
     1681 tctcttgttc agcgctgaca ctgttttccc gtttgccgcg ccggtctcgg cagcctggct
     1741 ctcggtcgtc ggtgcggcaa gctatcagca ctttgtcgtg cggcgccgcc ttgaaaaaac
     1801 cgaggccgag cgcgcgcgct atcagcaggc ggtccacttc gtcactcacg agatgcgaac
     1861 gccgctcaca gccatccagg gatcgagcga gctgatgacc cgctacagtc tcactgagga
     1921 taagcgtaag cagatcgccg ggctgattca ctccgaatcg cggcgtctcg cgaagatggt
     1981 ggagacgttc ctcaatgtcg agcggctctc cgcgggtcaa atggagttgc gtagggagcc
     2041 gttctccgcg ccggagatcg tcgccgcctg cgccgaaagg gcccggcccc tcgccgagaa
     2101 gaaacagatc cgtctccgag tcggcgaggt ggtggaccaa acgctcattg gcgaccgcga
     2161 actcatggag tacgccgtct ataatctgat caataacgcg atcaagtact ctccctccga
     2221 gacggaggtg accgtgacgg gtcgccggca aagcggcctg ttccgcctca ccgtcaggga
     2281 ccagggaatc ggcatggacg aaaacgagat caagaacctc tttcagaagt tctatcgcac
     2341 acgcagggcg atcgcttctg gagagtccgg aaccggtatc ggtctttcaa tcgtggagca
     2401 gattgtcacg caccacggcg gccgtatcga ggtggagagc gagcccggaa aaggctcgcg
     2461 gtttacgctg gtgctgccgg cgacagtcac cgcgggaact ggtgggaggg aagtgtgagc
     2521 gataacacga acgccgcccg gctcctcgtc gtggaggacg acgctggagt acgcactacg
     2581 gtagtcacgt tcctggagct ggaaggctac caggtcgacg ccgtgtcctc cacgcgtgag
     2641 gccatccggc gcttgaacga gaccgcctac ccgattgtca tctcggatat ctacctcgac
     2701 gaaaaaaccg gcctcgacgt tctcgacgcg gcccgggcga aagaccccac ttgcgccgtc
     2761 atcctcatga ccgcccgagg caccatggag accgtaatgg ccgccacacg gggcggcgcc
     2821 ttcgactata tcgccaagcc attcgagctc gatgccctgt tggcgacggt caaacgcgcg
     2881 gaagccagcc gcgccggcgg cgtcgacgag gacgagtcgg aaatcgacga cctgcccgaa
     2941 tccgagatga tcggtagctc cgcgcagatg gtggccatct acaagatcgt ctcgcgcgtc
     3001 gcgccgacgg atgcgaccgt gttgatcgaa ggcgagaccg gtacaggcaa agaactcatc
     3061 gcccggatga tccacaccaa cagccctcgc gtcccgcagc cgttcgtggc ggtggactgc
     3121 gcttcggtcg ccccggctct cctggaaagc gagttgtttg gcgccatgcg cggagcctac
     3181 acaggcgcgg accgcgaccg aatgggcgtc ttcgaggccg ccaatcacgg caccgtgttc
     3241 ctcgatgaaa tcggcgacat tgaattgaac ttccagttga aaatgctgcg gttccttcag
     3301 gagcgcgaga tccggccgct tggctcacct cgctcccgtc cggtggacgt ccgcgtgatc
     3361 gccgctacga accgcgacct gcaggcgatg gtcgacgagg gcaagttccg cgaggacctt
     3421 tggttccgcg tcaacgtcgt ccgcatccac gttccgccgc tccgcgagcg ccggggggat
     3481 gtgcccctcc tcgcacattt cttcgttcgg aaatacaacg cgcgctacag tcagagcgca
     3541 cggctcacgg actcggcgct gcgcgcgatg gacgactatg tctggccggg caacgtgcgg
     3601 caactccagc acatgatgga gcgcctcacg atcctggcgc cgggcggccg catcgatcag
     3661 caagccatcc aagagtcaat ccgggcgacc gaaccgcacg aacacggcgg cgagaccctg
     3721 gccgaggcgg aaatggacca gatccgccgg gtcctgtccg ccaccggcgg caacaagagc
     3781 cgggccgcac ggatccttgg aatcgaacgc aaaacgctct accggaagct cgaacgggtc
     3841 ggcgaatagc ccccaggcgg cgcaaaaggt gtcattcgtg cgcgttttgg cgtcattttt
     3901 gtaccagatt gtgacttttt ttagtgcttt tccgcccgac tcggtctaca atctggatag
     3961 ccaggtctca tacatcgata cttccgagcg ggaggaccga gaccatgttt gacagcctag
     4021 acgaccagat gaagcacgac gcggaacagg aaacgacccg gggccagcgt tggctcctgg
     4081 tgttgacggt ggtcgtagtc tcgttgctcg ttttcggcgg cctgtatttt ggcgtccgga
     4141 tgctcgagta actctttttt cagagccggg acgctcccgg ctgaggcggc tcgtcagatg
     4201 cgagacgggc catgacggac ttgacgaggg ccggatattc ggcattggag ttatagacag
     4261 ataatttttc gtctaagtcc ttatttttgt atatcctaga gatagcgagc cgtcgcgcga
     4321 cgaggtggat gcctgcctgg atggagggga agtgtgcctt cccgttgtcc caaccgaaga
     4381 tattgttgtt cgtgaactcc ttcccccctc cggattcgat cacagaaatg ctcggaagca
     4441 ggcgccagtc caacccgtag tggtcggcag ccagcaggaa atccttggcc agatggtcaa
     4501 tcggggatcc gcggtcctcg aaaaaccgtt ttagaaccaa caacctaggg tcataatcgt
     4561 aggtcggcgg ctgaaagtgc tggccaggac tgagcgtgac attggtcgcg agcaagcccg
     4621 caaccatcac ggcagtacgc gacagcgtgg ttttcaagca gatcaccttc cttttcagag
     4681 acttaggtag gatttacgaa cggcggccca gagccaccca agacaccaga tattaggcca
     4741 ggatgaggcc caaacctatc gacatggggg cagtatagca cagctcggtt aagagccgct
     4801 gggcacaaaa aagccccggc gtgaggccgg ggcggcagga gtgaatgtga actgactgtg
     4861 agacttgggg cagccgtgtc agcggctgat aggtttcttc tgttcctcgc cggccgagac
     4921 ggccaccgcg gcgccggcac cggcgcccgc aatgacgact ccggcgatga tggctttggt
     4981 cgagcggctc atcgcggcgt tgccaccggg tttccgggac ctgctgccag ccgctggagg
     5041 gagggcggcc gtggcgcagg aaccgtgcag acggcgaacc ttagcggcct gcatgacttc
     5101 ggtagcgccg ggcgccggtt ggacgccctt gagaccggct ccggtgagct ccacctgaaa
     5161 gccggcgtgg tcattgaggt tcgggccgcg cacctccagc gtgacattcg cgaggcggtc
     5221 ggtgaggagg aagcgcccgt ctgtcttgac gaggcagccc gtgacagtga acggagccac
     5281 ggcgccagcg gcctggggct cgaattccag cgaggtcccg ggcgcaaggc tggcgagcaa
     5341 gacgccttcg gccgtcgtga cgcgcacagg agcggaggaa gcggccacga ggacgcgatt
     5401 ggcgccgttc aaagcgacgt atgcgtttct ctcgggtcgg atctgaaggc tgcgagcctc
     5461 gatccggaac cctgcaccgt tttcgacgtg actctcgcct tcttcaa
//
LOCUS       DATIFQ010000008         3187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2301150, whole genome shotgun sequence.
ACCESSION   DATIFQ010000008 DATIFQ010000000
VERSION     DATIFQ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2301150"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VLE22_00375"
     CDS             complement(<1..341)
                     /locus_tag="VLE22_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12877.1"
                     /translation="MDWYRAIETAEKIVTILAIIGGAGWAYFHYFRGRTYRPRLEPKV
                     DGKVFIRVGVRYVIATAELKNAGLSKLEIMQEGTALRVLAGQPTQDATDIEWARLCTL
                     PVFETHRWIRS"
     gene            complement(619..2790)
                     /locus_tag="VLE22_00380"
     CDS             complement(619..2790)
                     /locus_tag="VLE22_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HSB12878.1"
                     /translation="MRATRLTRMLPSIALLVGATSPMNGQALTYSTYVGGTERRAFHQ
                     PVNAVAVDSAGNAYITGTTQSADFPVTPGALQTVFGGGACSVADGVFFPCSDAFVTKF
                     DRTGKVVFSTFLGAGRGDSGGGIALDTAGNIYVTGGAGLNFPTTAGALHDTTGVYIAK
                     LNRDGTKLLSSAVVDLGGWVSVNGFAVDAAGNAYLAGQDFLGPGGDAFVAKVNSTGSA
                     IVYKTYLRGAATDLARDVAVDLVGNAYVTGETFSTDFPTTPAAFQHTSLGLGAAFVAK
                     LNPGGGVVYSTYLGGTRGASGAGIAVDGAGAAYITGSVSAPPTESHNFHTTPGVPQPS
                     ASPLGTSHGFVAKLDAAGSALVYATFLGGNGEDEGSRIAVSSSGEAHVVGSTSSTNFP
                     RSPGGYPYLSGIGGSNAFLTKLNANGTAFLYSTCLGGMGTTGARGIAIDPTRATAWVA
                     GQTNSPEFPTTPDALQPAFPLNTTGFASKFDFSASAGMITMGVVNSASLLPAPIAPGE
                     LVTIFGAGVGPQTPAFLELDADGKVATTLAGVRVTFDGWPAPLTYVSSGQINAVVPYA
                     VNGKTNARLQVDYNGQKSDSVVIAVADTAPGVFTVTMHGRGQGAILNQDGTLNSPANP
                     AEKGSIVSIWANGMGQTDPPGEDGKITTAPLPVPRSPVYVWINGIQAPEITYAGAAPQ
                     LVSGVLQVNVRLPLGIPSGSAVAIALTMGYAASPWVTLAVK"
     gene            complement(2999..>3187)
                     /locus_tag="VLE22_00385"
     CDS             complement(2999..>3187)
                     /locus_tag="VLE22_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12879.1"
                     /translation="NLAYSPPRSKPTASGELAPVANRVRAGHVRLAHFGPPEFRTREI
                     AAMLLEDMVLRPVQGDVQ"
BASE COUNT          645 a          949 c          968 g          625 t
ORIGIN      
        1 tccgatctaa tccacctgtg cgtctcgaaa acgggaagag tgcatagacg cgcccattcg
       61 atatccgttg cgtcttgtgt tggctgcccg gcaagaactc gaagcgccgt accctcctgc
      121 atgatctcga gcttcgagag cccggcgttc ttgagctcag ctgtcgcaat cacgtaccgc
      181 accccgactc ttatgaaaac cttcccatcg acctttggct ctagccgcgg cctgtaagtc
      241 ctgcctctaa aatagtgaaa atacgcccaa ccggcgccac cgataatggc gaggatcgtc
      301 actatctttt cggcagtctc gattgcccgg taccagtcca tcgcggcgtt tccaaaatgt
      361 cccgtcgaga tatcataacc tgacgacgac acgcgcgaag gggagccgca gcggttgggt
      421 gaccgaagcg gattttcctc agacgcactc gcggtcttct cctccgctgg tggcagaggg
      481 gcacagcctc ttgcctgcag gtgttgcgct ccggagcgtc ttcatcgcgt gagacccgca
      541 tggataaagg cgtcggaggc gatcaggaag ttatcagtaa gtggtcaaat cggaaatgcg
      601 gggtaatatt tgcgcggcct atttcacggc cagcgtgacc caagggctcg cggcatagcc
      661 catggtaagc gcaatcgcca ccgcgcttcc cgaaggaatg ccgagcggta ggcgaacatt
      721 cacctgcaag acgcccgaga ccagttgcgg ggcggcaccg gcgtaagtta tctccggcgc
      781 ctgaatgccg ttgatccaaa catataccgg actgcgcgga acaggcagcg gagccgtagt
      841 gattttccca tcctccccgg gtgggtccgt ctggcccatg ccgttagccc agatgctcac
      901 gatcgatcct ttttccgcgg gattcgcggg tgagtttaac gtaccgtctt gattcagaat
      961 cgcgccctgg ccgcggccgt gcatcgtcac cgtgaacacc ccaggggcag tatcggccac
     1021 ggcgatcaca acagaatctg atttctgtcc gttgtaatcg acctgcaaac gggcgttcgt
     1081 cttcccgtta acagcgtatg gcaccactgc gttgatctgg ccggaagata catatgtcaa
     1141 gggcgcgggc caaccgtcga acgtgacgcg aacgcctgca agcgtggttg ctacttttcc
     1201 atcggcgtcg agttccagaa atgcgggagt ctggggtcca acgccagctc caaagatcgt
     1261 cacaagttca ccgggtgcga tgggcgcggg aagcagactc gcgctgttaa cgacgcccat
     1321 agtaatcatt ccggccgagg ccgagaagtc aaacttagag gcaaaaccgg tagtgtttag
     1381 tgggaacgca ggctggagtg cgtccggggt agtcgggaat tcgggtgagt tggtctggcc
     1441 cgcaacccaa gccgttgccc gtgtgggatc aatggcaatg ccacgcgctc cggtcgttcc
     1501 cataccccca agacaagtcg aatacaggaa ggcggttcca ttcgcattca gttttgtgag
     1561 gaaggcgttg ctgccgccaa tgccactcag atacgggtag ccgcccggac ttcggggaaa
     1621 gttggtggaa cttgtgctcc cgacgacatg tgcctctccc gaggaactga ccgcaatccg
     1681 agagccttcg tcttctccgt tgccgcctaa gaacgtagcg tagacaagcg cactgccggc
     1741 ggcgtccagc ttggccacaa atccgtggct tgtgccgagc ggggaagcgg acggctgagg
     1801 cactccaggc gtggtgtgaa agttgtgcga ttccgtcggc ggcgcactca cggagccggt
     1861 gatgtaggct gccccagccc catcaacggc aattcctgca cctgatgcgc cccgggttcc
     1921 tccgaggtag gtcgagtaga cgaccccccc accagggttc agtttcgcaa cgaaggccgc
     1981 cccgagacca agcgaggtat gttggaacgc cgcgggtgtg gtcggaaaat ctgtcgaaaa
     2041 cgtctcgccg gtgacgtagg cattgcccac caagtcaaca gcaacgtctc gcgcgagatc
     2101 cgtggcggcg cctctgagat aggttttgta cacgattgcg ctgcccgtcg agttcacctt
     2161 cgccacgaac gcatccccgc cggggccaag gaaatcctgg cccgcgagat aagcgttacc
     2221 ggcggcatct acggcgaatc cgtttacgga aacccaaccc ccgagatcga ctacggccga
     2281 cgatagaagt ttcgtcccgt cacggtttag cttggcgatg tatacgccag ttgtgtcatg
     2341 gagggcgccg gccgtagtcg ggaagtttag ccccgctcca ccggtcacat agatgttccc
     2401 cgcagtatcg agtgcgatgc cgccgccgga atcaccgcgg ccagcaccga ggaatgtcga
     2461 gaacacgacc tttcctgtcc ggtcgaactt ggtgacgaag gcatccgagc aggggaagaa
     2521 aacgccgtcc gccacgctac aggcgccgcc gccgaaaacg gtctgcaatg ctccaggtgt
     2581 caccggaaag tcggccgact gagtggtgcc cgtgatatac gcgttcccag ccgaatcgac
     2641 ggcaaccgcg ttgacgggct ggtggaaggc acggcgttcg gttccaccga catacgtgga
     2701 gtatgtcagg gcctggccgt tcatggggga tgtcgcacca acgaggagtg cgatggatgg
     2761 caacattcgg gtgagtcgcg tcgctcgcat tttgttctcc ggcgaccaag agtcaggctg
     2821 atcggctcga gatatcgtcc accacgtagc tatgccggtg caatccgcag gccatcggtc
     2881 caagacggaa gtgcgcgata gtgctcgctc gattccgcac ttgtctgcgg ttagttgtgg
     2941 ctttccgcca caaccgatgt gtacggcgca cacgcaggct cgcctggcaa tcgggcggct
     3001 attggacgtc gccttgaacc gggcgaagga ccatatcctc aagcaacatg gcggcgattt
     3061 ccctggtcct gaactcggga ggtccgaagt gcgctagccg cacatggccg gcacggacac
     3121 ggttggcgac cggggcgagt tctccggatg cagtcggttt gctcctcggg ggagagtatg
     3181 caagatt
//
LOCUS       DATIFQ010000009         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2301223, whole genome shotgun sequence.
ACCESSION   DATIFQ010000009 DATIFQ010000000
VERSION     DATIFQ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2301223"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VLE22_00390"
     CDS             complement(<1..206)
                     /locus_tag="VLE22_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12880.1"
                     /translation="MMPIGLVMLAQAIFGQAGPEGVSYIRFLGGKIDGEITAVAVDPV
                     GNTYVGGDRFSAYPGERVPSDFVLT"
     gene            complement(772..2283)
                     /locus_tag="VLE22_00395"
     CDS             complement(772..2283)
                     /locus_tag="VLE22_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HSB12881.1"
                     /translation="MMTRRAAVSLLAAPLVARDAKREFDPDFGSALEAAAAIGARKIS
                     AVELTRHVCERIDKFQPKLNAFVYQTREEALASARRADEAIARKQKVPPFAGVPMVVK
                     ESFGVAGRPCTWGIPALKDTRAPANSTCVQRLLDAGAVLVGATNVPFQLMDGQSYNEI
                     YGTTNNPWDLTRTPGGSSGGTAAALAAGLGFLGLGSDIGGSIRAPAHCCGIFGHKPTL
                     DVVSLRGHAPGGVLEDPGFSTLLAAPGPMARTAEDLEAAMRVLGGPELPDSVAFTWRL
                     PAPRHERLRDFRIGYVLEDPMMRVSSEVKPALESAVRALEKAGARLQPGWPAGFSISE
                     LFSHYLAMLFAFLFSVAPPEEQAQQRAQFGARKDDPQAAASLIDFASWQRHNLSRIAF
                     RAAWRQYFRNFDAFLLPALPVPAFPHDHTEQARRTLTTPEGPLPYLQALLSYMSVAIL
                     TGCPATVAPAGKTAKGLPVGIQILGPYLEDATPIRLASLLAKENGGFTPPQGY"
     gene            complement(2386..>2585)
                     /locus_tag="VLE22_00400"
     CDS             complement(2386..>2585)
                     /locus_tag="VLE22_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12882.1"
                     /translation="MVLTLASDEVREVHNALLDAHSEVLRKLSLEGFPSRELGLELCR
                     RKWKLEALLHQLDHPGSLIPF"
BASE COUNT          505 a          799 c          785 g          496 t
ORIGIN      
        1 gttaggacga agtccgatgg cactcgttct ccggggtacg cggagaagcg gtcgccacca
       61 acgtatgtat taccaacagg gtcaacggca accgccgtta tttctccatc gatcttgccg
      121 ccgaggaatc gtatgtagga gacgccttcc ggtcctgcct gcccgaaaat cgcttgcgcc
      181 agcatcacga gcccaatagg catcattctc attacactca ttggaccaaa acggcgaacc
      241 ccaagttccc gctccgatgg atatccaggg ataccgtcga atgacctcgt ctctaaagtt
      301 cacccgatcc gttgcgcctg ctggcgagtg aacaccctcc tacctgcgga cgatcagtcg
      361 caatacctcg atcgccaaca tccgcctgta gccgttttgg gcggtgctgc tatatggcga
      421 tttacagatc ctgtctgagc aacatatttt gctcaggttt ttgggatggc tacgcgaccg
      481 cgcgcgtgcg cgcgaaagca cgtcgaacac cagtgcggaa gccagggtcc aagcagggtc
      541 cgtttcgcca cagcttcggc catttagggt ccaattaggg tccaaacaga agacactcaa
      601 gtcttttttt gcagtaagcg atgctcgtaa attgttgatg tttcctactt cgggaccagg
      661 aggtcggtgg ttcgaatcca ctcgccccga ccattctttt ccgcggcggc tctacaccaa
      721 tcccgaacga atcgaccccg ttgtggcttc catgctgttg tgctgctacg ctcagtagcc
      781 ctggggtgga gtgaagccgc cgttctcttt ggcgagcagg ctcgcgagcc ggattggggt
      841 cgcatcttcg agatacggtc ctagaatctg gatccctacc ggcagcccct ttgccgtttt
      901 gcccgccggc gccaccgtgg ccggacaacc tgtcagaatc gccacactca tatagctgag
      961 cagggcttgc aggtatggaa gcggtccctc cggcgtagtc agcgtgcgac gggcttgttc
     1021 ggtgtgatcg tgtgggaagg ccggcaccgg gagcgccggc aacaggaatg cgtcgaaatt
     1081 tcgaaaatac tgccgccaag ccgcgcggaa cgcgatccgg ctcaggttgt ggcgctgcca
     1141 cgacgcgaaa tcaataagcg aagccgccgc ttgtggatcg tcttttcgcg cgccaaattg
     1201 cgcgcgctgt tgcgcctgct cctcgggtgg cgcgacgctg aacaagaacg caaagagcat
     1261 ggcgaggtag tgggagaaga gttccgagat ggaaaagccc gcgggccaac caggctgcaa
     1321 ccgcgcgccg gccttctcga gcgcacgcac ggcggattcc agcgctggct tgacctcgga
     1381 agacacgcgc atcatcggat cctcgagcac gtagcctatc cggaaatcac gaagccgttc
     1441 gtggcgcggc gccggcaggc gccaagtgaa tgcgacggaa tcgggcaatt ccggcccgcc
     1501 gagcacacgc attgccgcct caaggtcctc cgccgtccgg gccatcggtc ccggcgctgc
     1561 cagcagggtc gagaatcccg gatcctccaa cacaccgccg ggggcgtggc cgcgaaggct
     1621 caccacgtcg agcgtcggtt tgtgaccgaa gatgccgcaa cagtgcgcag gggcacgaat
     1681 gctgccaccg atatcgctgc caagaccgag gaaccccagg ccggccgcca gcgccgcggc
     1741 ggtgccgcct gaggagccgc cgggagttcg agtcaggtcc cacggattgt tcgtggtccc
     1801 atagatctcg ttgtagctct gcccatccat caactggaag ggaacgttgg tcgccccgac
     1861 caggaccgcg ccggcgtcca gcagccgctg cacgcaagtt gagttcgcgg gcgcgcgagt
     1921 atccttcaag gcgggtatgc cccaggtgca cggacgtccc gcgacaccga agctctcctt
     1981 caccaccatc ggcacgccgg caaacggcgg gaccttctgc ttgcgcgcaa tagcctcgtc
     2041 agcgcgccga gcgctggcca gcgcttcctc gcgtgtctgg taaacgaagg cgttgagctt
     2101 gggttggaat ttgtcgattc gctcgcacac atgcctagtc agttcgacgg cagagatctt
     2161 acgagcgccg atggcggctg cggcttcgag cgcggagcca aaatccgggt cgaattctct
     2221 tttggcgtcc cgtgctacca gcggagccgc caggagagaa accgctgcac ggcgagtcat
     2281 cattgtcgga atggtatcat ctctgcgccg gatgcggctc tccggtcagg aagaaggagg
     2341 agccgttggg gagatctcag tagaggctgt tcgcgggaac cgattctaga acgggatgag
     2401 ggagccaggg tggtctaact ggtgaagcaa ggcctccagt ttccacttcc tccggcacag
     2461 ttccagtccg agctcgcggc tcgggaagcc ttccaggctc aacttacgaa ggacctccga
     2521 atgtgcgtcc aacagcgcgt tatggacttc ccggacttcg tcgctggcga gcgtcagaac
     2581 cattt
//
LOCUS       DATIFQ010000010         3488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1534865, whole genome shotgun sequence.
ACCESSION   DATIFQ010000010 DATIFQ010000000
VERSION     DATIFQ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534865"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VLE22_00405"
     CDS             <1..1242
                     /locus_tag="VLE22_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HSB12883.1"
                     /translation="SAIIVKAARWLVRFTFPLWLVFLAIPLAAQHGSRAAEIEAARDE
                     KAKGLTPDDPSRAEQLLVEIKEKRILERLTAGIAGFRIKFGGLVTGSGFALGPEYLRT
                     DLADGNIILRSSARGSLKKYTLLDFELSTPRLAGDRLFLDFYAAHRNYPRMQYYGPGP
                     DSAKTGRSNFRLENMAWDFSGGVKPVRNLKVGATGGFLKVNVGPGTDRRFVSAEQIYT
                     ESMTPGIDRQTDFARTGVFAQYDYRDLPGGPRRGGNYLVRYSWHFDRRLDLNSFRQLD
                     LEVQQYIPFFNERRVIALRGKSVLTYPGDGQVVPFYLQPTLGGSDDLRGFRPFRFYDD
                     NLTVFNAEYRWETFSGLDMAVFADAGKVFHRRGDWNLHDLEGCYGVGMRFNVRNSVFL
                     RIDAGFSHEGFQVWLKFNNVF"
     gene            complement(2722..>3488)
                     /locus_tag="VLE22_00410"
     CDS             complement(2722..>3488)
                     /locus_tag="VLE22_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB12884.1"
                     /translation="SLGAKDHVLGSLPLGWKLILGVIGYTGDRERGRQTLEFVARNGT
                     LLADDARILLILIHTRQKDYQKAYDYLAELQQKYQQNYLIRLDMGGMELLMNRPERAI
                     AIYGEVLRRRNTGERNYSGLELAFLCNRLGVAFRQAGDLEGAVGWFRKALAENGRSSR
                     STTVARLELGKTFDLMGRRVEAVEQYQAVLSAEDVAGSKWEAKSFLKTSFQPANDYQN
                     RSRPTTRRPAASPRRRYTVTLFFGSEPRISAARYSA"
BASE COUNT          727 a         1007 c         1053 g          701 t
ORIGIN      
        1 agcgcgataa tagtgaaagc ggcgaggtgg ctggtgcgtt tcacgttccc attgtggctg
       61 gtgtttcttg cgattcccct tgccgctcag catggatcaa gagccgcgga gatcgaagct
      121 gcgcgcgatg aaaaggcgaa ggggctgact ccggacgacc cctctcgcgc tgaacaactg
      181 ctggtcgaga tcaaagagaa aagaatactg gaacgcctga ccgctggcat cgccggattt
      241 cgcatcaagt tcggcggctt ggtgacggga tcgggatttg ccctcggtcc cgagtaccta
      301 cgtacggatc ttgcggacgg caacattatc cttcgcagct cggcgcgagg gtcactgaag
      361 aaatacaccc ttctggactt cgaactctca acgccacggc tcgccggcga tcggttgttc
      421 ctggacttct atgccgcaca ccgaaactac ccgcgaatgc agtactacgg accgggaccc
      481 gactcggcga agaccgggcg gagcaacttc cgtctggaaa acatggcgtg ggatttctca
      541 gggggcgtca aaccggtcag aaaccttaaa gtcggcgcga cgggtggctt cctgaaagtg
      601 aatgtcggcc ccggcacgga cagacgcttc gtgtcggccg aacagatcta cactgagtcg
      661 atgacgccag gaatcgaccg gcagaccgac tttgcgcgga ccggtgtctt tgcgcaatac
      721 gattaccgcg accttccggg cggtcccagg aggggaggca actatcttgt gcgatacagt
      781 tggcacttcg accggcgact ggacctgaac tcgttccggc agctcgatct cgaagtgcag
      841 cagtacattc cgttttttaa tgagcgccgg gtgatcgcgc tgcgcggtaa gtccgtgctg
      901 acgtaccccg gggacggtca ggttgttccg ttctaccttc agccgacgct cggcggctcc
      961 gatgatctgc gaggctttcg ccccttccgc ttttatgacg acaacctgac agtgttcaac
     1021 gccgagtacc gatgggaaac tttcagcgga cttgacatgg cggtgttcgc ggacgccggc
     1081 aaggtgtttc accggcgcgg ggactggaac ctccatgacc tggaaggatg ctacggcgtc
     1141 ggaatgcggt ttaacgtccg aaattcggtg ttcttgagga tcgacgccgg cttcagccac
     1201 gagggttttc aagtgtggct gaagttcaac aacgtgttct gaggatgcca tggtgaagcg
     1261 aattgtcttg ctcacgttgc tgctggcagc cttgctcggt gcggaaaaat tctatccgga
     1321 cgacccgatg ggaagagaac cggcgccgca cagagttgaa agagcgaaac ggcggaagct
     1381 cagtgatatt tacgactttt tccaaaacac attcgcgagg ccgggcgagg cccaaacacg
     1441 cgggagacct ctgccggcgc agggcgtcaa cacactcggc gaagtaccgg acggcgcctg
     1501 gtacgtgaat cgtcacggca aagctcccat ggccctggag gagcttacgc gaggtccatc
     1561 gagcgccaac ccgccttcga tggatggcac ttgggacatc gaatccgcaa aaagcgaggg
     1621 cgtcactcct ggtttcgtga tcctcgactc aaggggaacc cggtactggc tgaagttcga
     1681 tccgctgacc aacccggaga tgacgagcgc tgcggatgtg atcggctcga aattctttca
     1741 cgcgctcggc tatcacgttc cggagaacta tctcgttgat ttgaaacgcg ataagctccg
     1801 gctaaccggg aaatcacagc ttcgggattc actagggaag aggcgcccga tgacgtcacg
     1861 cgacgtaacc gaggtccttc tcagagcccc gcaaaacgga gacggcagct atcgcgcggt
     1921 ggccagtctg ggaattgccg gccaggaact gggcccgttt cgctactacg gtacgcgcaa
     1981 ggacgatccc aacgatatcg tgccgcatga acaccggcgg gacctgcgag gcttatttgt
     2041 gttttgcgct tggcttggac acgacgactc gcgggccatc aatactgacg acttcctcgt
     2101 ggaggagaac gggctgaaat acatcaagca ttacctgatc gatttcggtt ccatcctggg
     2161 aagcgccagc gagaaggcga acagtgcccg ttccggcaac gagcacctgt ttgcatggcg
     2221 gccggcggcg ctccagttct tctcacttgg gctgttcgtc ccgcggtggg cgaaggctga
     2281 ctttccagac ctgccgtctg taggccggtt tgagtccaga atcttcgatc cagataagta
     2341 caagggtgag tatccgaatc cggccttcta caaccgcctt ccggacgata cgttttgggg
     2401 cgccaggcaa gtgatggcgt ttaccgacga acagatccgg gcgatcgtca agacgggagc
     2461 gtacagcgac ccggcagcgg agcaatgggt tgtgaagtgc ctgatcgagc ggcgcaacaa
     2521 gatcggaaag gtctacttcc agaaggtgct acccctcgac cggttcgaat gtaagaacgg
     2581 aaggctggag ttcgaggacc tggcggcgaa gttcggattc gctcagccac agaaactatc
     2641 gattcagtgg ttccgattcg acaacgagac cgggaacaaa tctccgcttg tgggcgaagc
     2701 aagccttacg gtcccaacga atcaggcgga atacctcgcc gcggagattc gcggctccga
     2761 gccgaagaaa agcgtgactg tgtatcttcg ccggggggag gcggctgggc gccgcgtcgt
     2821 gggtctcgag cgattctggt aatcatttgc gggctggaaa gacgttttca gaaacgactt
     2881 cgcttcccat ttcgatcccg ccacgtcctc cgcgctaagg accgcctggt attgttcgac
     2941 agcttccacg cgtctaccca tgaggtcgaa cgtctttccg agttcgagcc gcgccacggt
     3001 ggtcgagcgg ctggaacgcc cgttctcggc aagggccttg cggaaccatc cgacggcgcc
     3061 ctccagatcg ccggcctgcc ggaacgccac gccgagccga ttgcagagga acgccagttc
     3121 caggccggag tagttccgtt ctcccgtgtt tcgccgtcta aggacctccc cgtagattgc
     3181 gatcgcacgc tccggccggt tcatcagcag ttccatgccg cccatgtcca gccgtatcag
     3241 gtaattctgc tggtacttct gttgaagctc cgccaggtaa tcgtaagcct tctgatagtc
     3301 cttctgtcgt gtgtgaatca gaatcaacag gatgcgcgca tcgtcggcca gcagagtgcc
     3361 gttccgcgcc acaaactcaa gtgtctgccg gccacgctcc ctgtcaccgg tatatccgat
     3421 cacgccgagg atcaacttcc acccgagcgg aaggctgccc aaaacatggt ctttcgcgcc
     3481 gagggaca
//
LOCUS       DATIFQ010000011         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1918723, whole genome shotgun sequence.
ACCESSION   DATIFQ010000011 DATIFQ010000000
VERSION     DATIFQ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918723"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..718)
                     /locus_tag="VLE22_00415"
     CDS             complement(374..718)
                     /locus_tag="VLE22_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12885.1"
                     /translation="MNTPAIPKAAGLIALCALSFARGLPARAEHSNEELAKAAQNPVA
                     NLISVPFQDNINFNYGPEEKTQNVLNIQPVLPVSLGPDWNLITRLADRHRRLEGAEQR
                     RRLDRTARRRNR"
     gene            complement(864..1142)
                     /locus_tag="VLE22_00420"
     CDS             complement(864..1142)
                     /locus_tag="VLE22_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12886.1"
                     /translation="MKIHYLSITGFAGLLLATTAHAGSITCGDSIITDDQPDGQFQAQ
                     ILQQCGEPTSKDGDDWFYDRSDVGQGMYVLHFDDSGQLDSIEQQMDQE"
     gene            complement(1352..>2259)
                     /locus_tag="VLE22_00425"
     CDS             complement(1352..>2259)
                     /locus_tag="VLE22_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HSB12887.1"
                     /translation="GSYFGLGAKFDDTWAGRYYIQEEDFLTSFANLALGYRVNDWLSV
                     GGGLSLVYGELGAQTNINNALDALPDGRMKLQANDTDIGWNAGLLIEPRSGTRVGLTY
                     ISKVDLKYKDQISFHGLGPGLDAALQLSGLKGAETTFDFTLPNQVMLSGYHELTPDLA
                     LMADLGWQNWSQFGQIGLSIDSTTSTSTTVDAHFQDTWHTALGARYRLNPLWSVSAGF
                     AYDSSPATNSHRSIVMPLDRQYRYAMGLTYDWRKNITFGVAYELMDAGSAPVNQTGGP
                     LKGDLKGRFKDDAFNILALSANWKF"
BASE COUNT          443 a          640 c          691 g          485 t
ORIGIN      
        1 atagccacgg aaccacacgg aaaaacacgg accataccga aaatttccgt gtcttccgtg
       61 tgcttccgtg gccaattgta gaatgtaggg tgggttagcg tttttcgcgt aacccaccag
      121 gcgttgcgca gcaacacaac gcattttttg atgagtgcct tcggcacagc cggtgggtta
      181 cggcacaaac tacgtgccta acccaccctc catcgattag ggaaccgctc ctgtctgatg
      241 gtgggatagt catttcggga acagcaattg cgcctgcagg cgccaggtcc agttcgggcc
      301 gaactccggc ttctcgacat tgtagaaggc ctgggtctgg aggttcaccg gcaatcttcc
      361 caggtgcacg attttaccga ttccgccgcc gagcggtacg gtccagacgt cgccgctgtt
      421 ctgcgccttc cagtcggcgg tgacgatcgg cgaggcgggt gatcaggttc cagtcgggtc
      481 ccaggctgac cgggaggacg ggctggatat tcagcacgtt ctgggtcttc tcctccggcc
      541 cgtagttgaa attgatgttg tcctgaaagg gcacgctgat gaggttggcc acgggattct
      601 gcgccgcctt ggccaattcc tcgttgctat gttccgcacg ggcgggtaat ccccgggcga
      661 atgacagggc gcagagtgcg atcaggccgg ccgcttttgg gattgccggt gtgttcatac
      721 cacgacagga aaggttctta aacagcattt ttaccgcaaa gacgcaaagt tcgcgaagta
      781 agagtcatta aacagcttgg cgttctttgc gcctttgcgg tgagcttatt ttttgtgggt
      841 gagcgcgagt cagcccgcgg cccctactcc tggtccatct gctgctcgat ggagtcgagc
      901 tggcccgaat cgtcgaagtg cagcacgtac attccctgcc ccacatccga acggtcatag
      961 aaccagtcgt cgccgtcctt ggaggtcggc tctccgcact gctggaggat ctgcgcctgg
     1021 aactgcccgt ccggctggtc atcggtgatg atcgaatcgc cacaggtgat ggagccggca
     1081 tgcgcagtcg tggcaagcag cagtcccgcg aatccggtga tggaaaggta atggattttc
     1141 atggtgatat ctcccgttgt tgtttgatga ttggtaactg ccccgggtat gcggcggccg
     1201 gcataccggt caccgattct agtgctttca tagtaggagc gagcttgctc gcgaaaagtt
     1261 ctattgaaac acccgattcg cgagcaagct cgctcctaca gagaaaaata gcaaatttct
     1321 tggcgcccgt aaggtctctt cccccacgag gctagaactt ccagttcgcg ctgagcgcca
     1381 ggatgttaaa ggcatcatcc ttaaaccggc ccttgagatc accctttaac gggccgccgg
     1441 tttgattgac cggcgcgctg ccggcatcca tgagctcgta ggctacgcca aaggtgatgt
     1501 tcttgcgcca gtcatacgtt agacccatcg cgtagcggta ctggcggtcg agcggcatga
     1561 cgatggaacg gtgggaattg gtggccggcg agctgtcgta ggcgaacccc gcggacacgg
     1621 accacagcgg attgagccgg tagcgtgccc ccagggccgt atgccaggtg tcctggaagt
     1681 gggcgtccac cgtcgtgctg gtcgaggtcg tggagtcgat cgacaacccg atctggccga
     1741 attgcgacca gttctgccag ccgagatccg ccatgagcgc gaggtcgggg gtcagttcat
     1801 ggtagccgct cagcatcacc tgattcggga gggtgaagtc gaaggtagtc tcggcaccct
     1861 tcagcccgga caactgcagg gcggcatcca gcccgggacc gaggccatga aacgagattt
     1921 gatccttgta tttgaggtcg accttggaga tataggtgag acccacccgc gtgccgctcc
     1981 ggggttcgat cagcaggccg gcattccagc cgatgtccgt gtcgttggcc tggagcttca
     2041 tgcggccgtc cggcagcgcg tcaagcgcat tgttgatgtt cgtctgagcg ccgagttccc
     2101 cgtagaccag cgacaggccg ccgccgaccg acagccagtc gtttacccgg tagccgagag
     2161 caaggttggc gaagctggtg agaaaatcct cctcctggat atagtagcgc ccggcccagg
     2221 tatcatcgaa cttggccccc aggccaaaat aggaaccga
//
LOCUS       DATIFQ010000012         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2302187, whole genome shotgun sequence.
ACCESSION   DATIFQ010000012 DATIFQ010000000
VERSION     DATIFQ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2302187"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VLE22_00430"
     CDS             <1..616
                     /locus_tag="VLE22_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSB12888.1"
                     /translation="GLPGDYENLKRLLAPLAEKTPVCAALGNHDDRRNFLAAFTATPG
                     KRQPVEGKFVSVLEHGPLRFVFLDSLMEPDVTPGFLGKRQRMWLENSLAAGDGKPVLV
                     FVHHTLGDGDNDLLDVEWLFRILVPARQVKAVVYGHSHRYHFDTRDGIHLINIPAVGY
                     NFTDSEPVGWVEAALTADGADFKLRATGGNREKDGKVTSVSWRG"
     gene            670..858
                     /locus_tag="VLE22_00435"
     CDS             670..858
                     /locus_tag="VLE22_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12889.1"
                     /translation="MRRKLTDVVVDALRRLIGRHVRQVCPPRTGFTVVGGRGLQPGVD
                     LDNTADLLDLMERAEGAR"
     gene            complement(902..1348)
                     /locus_tag="VLE22_00440"
     CDS             complement(902..1348)
                     /locus_tag="VLE22_00440"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HSB12890.1"
                     /translation="MPLLERIQTEMVQAMKAREEARLSALRMIKTALKKHEVDSMKPL
                     DEAAEMQILNSLIKQRKDAAEMFRKGGRPEMADKEEAELRLIESYLPAAPSQEEMEAA
                     VAAALTETGVTSLKQMGVVIKAAQAKLAGKRVDGKVLSDKVRSKLE"
     gene            1383..>2045
                     /locus_tag="VLE22_00445"
     CDS             1383..>2045
                     /locus_tag="VLE22_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB12891.1"
                     /translation="MHVRKTRLLTPGPTPLYPPALHAMMGSDIHHRTEDFRNLYRATL
                     SDLKEVLGTSNDVLVLVSSGTGAMESAASNLFSRGDKVIVCSAGKFGERWVEIAKSFG
                     LEATVLKAEYGDAVRPEQVEAALAAEPGVRGVFVQASETSTGAAHDICAMGRAIAKTE
                     AIFVVDAITGLGTMPVDIDGWGLDVVIGGSQKAFMIPPGLAFLAISPKAWRLAETANL
                     PHY"
BASE COUNT          345 a          646 c          645 g          409 t
ORIGIN      
        1 gggacttccg ggcgactacg aaaatctgaa acgccttctt gccccgctcg ccgagaaaac
       61 gccggtttgc gccgcgctgg gtaaccacga cgaccggcga aacttcctcg ctgccttcac
      121 ggcgacgccc gggaagcggc agcccgtcga aggcaagttc gtatccgtgc tcgaacacgg
      181 gcccctccgg ttcgtttttc tggactcgct gatggagccg gacgtcacgc ccggatttct
      241 cggcaaacgc caacggatgt ggctcgaaaa ctccctggcg gcgggagatg ggaaaccggt
      301 gctggtgttc gttcaccaca cgctcgggga cggggacaac gaccttctcg acgtggagtg
      361 gctgttccgg atcctcgtgc ccgcgcggca ggtcaaggcg gtcgtctatg gccactcgca
      421 ccggtaccac ttcgacacgc gggacggaat ccacctcatc aatatcccgg cggtgggcta
      481 caacttcacc gattccgaac cggtgggctg ggtcgaagcg gcgctcaccg cggacggcgc
      541 cgacttcaag ctgcgcgcga ccggcggcaa ccgggaaaaa gacggcaagg tgacgtcggt
      601 gagctggagg gggtagctcg atccctgctc ttgctgcgcc ggacgcattt cggcaatcat
      661 ggtgtgatca tgcggcggaa gctcacagac gttgtagtgg atgcgctgag acggttgatc
      721 gggcggcacg ttcgtcaggt gtgtccccca cggacgggat ttactgtcgt cggcggacga
      781 gggctccagc ccggtgtcga cctcgacaat accgccgatc tgctggacct gatggagagg
      841 gccgagggtg cccgttgatg tcaacgtgct gtacggacgg gccacgtcgt ccgcgtgatc
      901 cctactccag cttcgagcgc accttgtcgc tgagaacttt tccgtcgacg cgtttgccgg
      961 cgagtttcgc ttgcgcggcc tttatcacca cgcccatttg cttgagcgaa gtgacgcccg
     1021 tttccgtgag agcggcggcg acggcggcct ccatctcctc ttgggagggc gccgccggca
     1081 ggtaggattc gatcagccgg agttcggctt cctctttgtc ggccatctcc gggcggccgc
     1141 ccttgcggaa catctctgcc gcgtccttgc gctgtttgat cagcgaattg aggatctgca
     1201 tttcggccgc ttcgtccagc ggcttcatcg agtccacttc gtgctttttt agcgccgttt
     1261 tgatcattcg gagtgcgctc aggcgggcct cttccctggc cttcatggcc tgcaccatct
     1321 cggtctggat tcgttcgagc aggggcatga cgtctgttat tctatagaac tacccgaaag
     1381 ccatgcacgt acgtaaaacc cgactcctga caccggggcc gacccctctg tatccaccgg
     1441 ccctgcacgc gatgatgggt tccgatatcc accatcgcac ggaagatttc cgcaatctat
     1501 accgggcgac cctaagtgac ttgaaagaag ttctaggcac gtcgaatgac gtgctggtgc
     1561 tcgtttcgtc cggaacgggc gcgatggagt cagccgcctc caacctgttc tcgcgcggcg
     1621 acaaagtcat cgtgtgttcc gccggcaagt tcggagagcg gtgggtcgag atcgccaagt
     1681 ccttcggcct ggaagcgacg gtcctgaagg cggagtacgg cgacgctgtc cggcccgagc
     1741 aggtcgaggc ggccctggcc gccgagcccg gcgttcgcgg cgtcttcgtg caggcttcgg
     1801 aaacctccac cggggccgca cacgatatct gcgccatggg gcgggcgatc gcgaaaaccg
     1861 aagccatctt cgtcgtcgac gccatcaccg gccttggcac gatgccggtc gatatcgacg
     1921 gctggggtct cgacgtggtg atcggcggct ctcagaaggc cttcatgatc ccgcccgggc
     1981 tggcgttcct tgcgatcagt ccgaaggcct ggcgcctcgc tgaaacagca aaccttccgc
     2041 actac
//
LOCUS       DATIFQ010000013         8278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_386681, whole genome shotgun sequence.
ACCESSION   DATIFQ010000013 DATIFQ010000000
VERSION     DATIFQ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386681"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VLE22_00450"
     CDS             <1..645
                     /locus_tag="VLE22_00450"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSB12892.1"
                     /translation="LIMGIRETPMSRGQMIQPLEQFGVRLMSMGFLSSGDRPLIWRGP
                     MLHSVVQQFLRGVEWGDLDYLIIDLPPGTGDVQLSLIQTAPITGAIVVTTPSDVSLED
                     ARKAVNMFRQVHVEVLGIVENMSYLVCPHCDKRVDVFSSGGGKRTAAEMGVNFLAELA
                     LDPEVRAGGDSGRPVALRDGNDPHAAGYVRMAEATVARITEVGAPTAPRMEISE"
     gene            complement(695..1972)
                     /locus_tag="VLE22_00455"
     CDS             complement(695..1972)
                     /locus_tag="VLE22_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12893.1"
                     /translation="MTLSRRNFIRTGTMAAAGAAAGFRLNAAASRAPQAYFGLHTFIE
                     SNPKAVFIRRTHVADKMDAAAVQAEGLSLARQIFVPMERAGVPVTHRIILKPNHTSIP
                     ERRRPLQENWGTGTDPHFYEGLVIGMKEIGLKKFHFIEANSFHLWNVRGWVDINERHG
                     IEMNECERRARHFKDGYELTWSKVSDGVIFKRIPHYAPVNEPDTWLFNIAKWKSHGMC
                     LTLTAKNEQGLVVLPFTRFCQGWQGVTGVSDIMKGDIQPEAEATVKKFFERHVRTYGR
                     YNSRAQLSPMHQEIWAHKTCDNTSVLKTGLAMIEGIVGRDGDGFGIGNDHLTNLVMFG
                     KDKFLVDVIGLYLGGHEPGNVHLYRIAKERGLCHTFNPWEIPVFEWTDQGPVARKLSE
                     FPRAALKTYYLQRESEPLFHLVNEPFDYDRYKI"
     gene            complement(1998..3281)
                     /locus_tag="VLE22_00460"
     CDS             complement(1998..3281)
                     /locus_tag="VLE22_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12894.1"
                     /translation="MPLDLSSDVVIRWDDLDPKHIPLLKQCGATAVLPPRVDAAFAEA
                     CRQAGLKTADPADIPLLGVEEMHKAPVKPAALKAGLWPGISRGGARGHNDEVASASRQ
                     PWIDANGFWIGYLRAMYPKQPAVLGYLPNEEAGLKPDRVVPHDSLELALIEAWANGGN
                     FLLAVEPRFCKALLEGDEKTGTAWRQLGRTAAWLREHRSLFGQPVLPVATALVEDDIT
                     AELVNLMYRQNVSPRLASAAAPPPPNPHCLALVAASISDPTPEARRKLLALAEAGCSL
                     VVDAPGEKAWWRAPELKHVRAQEDRDFFSLGRGQVIAYREQIADPSEFALDVIDIVTQ
                     KQRAVRLWNAHSVIALATAGPKTGPAPGAAVLCAINYGAPVDMDFPARIQGHYAKATL
                     LRPEAAPVPLKAVKRGTTTEVYVPELRRLGVIVFS"
     gene            complement(3321..4091)
                     /locus_tag="VLE22_00465"
     CDS             complement(3321..4091)
                     /locus_tag="VLE22_00465"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HSB12895.1"
                     /translation="MKITLHIWRQKNAHAPGRMVRYELKDVSPEMSFLEMLDVLNEEL
                     MTKGEDPVAFDHDCREGICGMCGAMVNGVAHGPQPGTTLCQLHMRHFKDGDAIYVEPW
                     RARAFPVIKDLVVDRSAFDRIIQAGGYISVSTGSAPDGNAVPIPKQIAERAMDAAACI
                     GCGACVAACPNAAAALFVGAKISQLGLLPQGQPERKRRALSMIAAMGEELFGSCTNIG
                     ACEAACPKSIKLEVIARMNRDFITASFTERPETVEFSG"
     gene            complement(4091..6004)
                     /locus_tag="VLE22_00470"
     CDS             complement(4091..6004)
                     /locus_tag="VLE22_00470"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HSB12896.1"
                     /translation="MKLDARIPSGPIEQRWDKYRFEMKLVNPANKRKFSVIMVGTGLA
                     GGSAAATLGELGYNVKAFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSIFRLFYDTVK
                     GGDFRARESNVYRLAQVSVNIIDQCVAQGVPFAREYGGMLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALMRQVAAGNVTMFPRTEMLDLIVIDGRARGIVTRNLITGEIDIHL
                     ADAVVLGTGGYGNAFFLATYAKGSNATAIWRSYKRGAGFGNPCFTQIHPTCIPVTGEY
                     QSKLTLMSESLRNDGRIWVPKEAGDKRPAAEIPEAERDYYLERRYPAFGNLVPRDVAS
                     RAAKQVCDDGRGVGETGLGVYLDFSDAIQRLGRHTIAERYGNLFEMYERITAENPYES
                     PMRIFPATHYTMGGLWVDYQLMSNVPGLFVIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIGDYLATAKLDKVGPDNPACREAEENVVAATNRLLSIKGKRTVDSFHRELGKI
                     LWEDCGMARNAAGLRQALEKIPALREEYWKNVNVLGTNEEFNQCLEKAGRVADFLELG
                     ELICLDALERNESCGGHFREEYQTPEGEALRDDEGYCHAAVWEYQGADRRPILHKEPL
                     KFEYVIPSQRSYK"
     gene            complement(6018..6701)
                     /locus_tag="VLE22_00475"
     CDS             complement(6018..6701)
                     /locus_tag="VLE22_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HSB12897.1"
                     /translation="MSSSAVSVRLDQATRFYQASIGKKVVMAVTGVILFGYVLGHLLG
                     NLQIYLGPEQINRYAEFLHSKPALLWVVRLLLLTAVVLHVTTSIQLWILKKRARPVGY
                     AKEENVPAGYASRTMLWSGPIIAAFVVFHILHLTTGSVNLPYRELDVYNNVVNGFRIP
                     AVSIAYIVAMVLLCMHLYHGVWSMFQTVGFSHPRYTPLLKRLAAGFAIFIAVGNISIP
                     VSVMAGLIR"
     gene            complement(6785..7306)
                     /locus_tag="VLE22_00480"
     CDS             complement(6785..7306)
                     /locus_tag="VLE22_00480"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB12898.1"
                     /translation="MDGKLAADFLRYSAEKLTQLSSRIQDCLDKLTYEQVWVRGADSE
                     NAVGNLVLHLCGNVRQWVGHGVGGQADVRVRDREFAARGEVQPVELSERMEGTIRAAL
                     DVLAKVTPERLAEKVTIQAYHVTVLEAIYHVVEHFSQHTGQIIFATKQLTGQDPGYYR
                     HLKGSAPHGETTP"
     gene            complement(7310..>8278)
                     /locus_tag="VLE22_00485"
     CDS             complement(7310..>8278)
                     /locus_tag="VLE22_00485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSB12899.1"
                     /translation="PPPAAPTPAAAPEEVAGPLSPLVRKMAREYNIDLRQVKGTGAGG
                     RITKQDVEAYIAAQAARTVAAPPPPAPVMPPPPAVPTEMPPVPRPEPARARVEPMSIM
                     RQKIAEHMVASKRTSAHVTTVHKIDMTAVAKVRQRNKELVKERYGFSLTFLPFVVRAA
                     AEALRAFPIVNASIEGANVIYHNEIHVGIAVALDNGLIVPVIRNADEKNVLGLQRAIV
                     DLAARARSRQLKPDEVHGGTFSITNFGSFGSLFGTPIINQPQVAILGVGAVDKTPVVV
                     EDDAIAIRHISLMGLTFDHRLIDGALADQFMQKMRAVLENWSEQVL"
BASE COUNT         1416 a         2742 c         2460 g         1660 t
ORIGIN      
        1 ctcattatgg gaatccggga aacgccgatg tcgcgcggcc agatgatcca gccgctcgaa
       61 caattcggcg tgcggctcat gtcgatgggc ttcctcagct cgggcgaccg tccgttgatc
      121 tggcgcggtc cgatgctgca cagcgtcgtc cagcagttcc tgcgcggcgt cgagtggggc
      181 gatctcgact atctgatcat cgacctcccg cccggcaccg gcgacgtcca actgagcctg
      241 atccagaccg ctcccatcac cggggcaatc gtggttacca cgccctcgga cgtctcgctg
      301 gaagacgctc ggaaagccgt gaacatgttc cgccaagtgc atgtcgaggt ccttgggatc
      361 gtcgaaaaca tgagctacct tgtctgcccg cactgcgaca aacgggtgga tgtgttcagt
      421 tccggtggag ggaaacgcac agcggcagag atgggtgtga acttcctggc tgaacttgcg
      481 ctcgatccgg aggtgcgcgc cggcggcgat tccggccggc ccgtcgcgct ccgcgacggg
      541 aacgaccccc acgcggcggg ttacgttcgg atggctgagg ctactgtcgc gcggattacc
      601 gaggtcggtg ctcccaccgc tccgcggatg gagatctcgg agtgattgcg ctgccgtagc
      661 atggcgcagg tctcccgacc tgccgtagag cttcttagat cttgtaccga tcgtagtcga
      721 acggttcgtt cactaggtgg aacagcggct cgctctcgcg ctggagatag taggtcttga
      781 gcgccgctcg cgggaactcc gagagctttc gcgccaccgg tccctggtcc gtccattcga
      841 agaccggaat ctcccagggg ttgaaggtat ggcacaggcc gcgttccttc gcgatgcggt
      901 acaggtgtac gttgcccggt tcgtggccgc ccagataaag gccgatcacg tccaccagaa
      961 acttgtcctt gccgaacatc accaggttcg tcaggtgatc gttgccgatg ccgaatccgt
     1021 cgccgtcgcg cccgacaatt ccctcgatca tcgccaggcc ggttttcaag acgctcgtgt
     1081 tgtcgcatgt cttgtgcgcc cagatttcct ggtgcatcgg actgagttgt gcccggctgt
     1141 tgtaccgtcc gtacgtgcgg acgtgccgct cgaagaactt ctttacggtc gcttcggcct
     1201 ccggctggat gtcccccttc atgatgtccg acacgcccgt cacgccctgc cagccttggc
     1261 aaaaccgcgt gaacggaagc acaaccagcc cttgctcgtt cttcgcggtc agcgtcaggc
     1321 acatgccgtg cgacttccat ttggcgatgt tgaacagcca ggtgtcgggc tcgttcaccg
     1381 gcgcgtagtg cgggattcgc ttgaagatca ctccatccga caccttgctc cacgtcagtt
     1441 cgtagccgtc cttgaaatgg cgcgcccggc gctcgcactc gttcatctcg atcccatggc
     1501 gctcgttgat gtcgacccag ccgcgaacat tccagaggtg aaacgagttg gcttctatga
     1561 agtggaactt tttgagcccg atctccttca tcccgatcac caggccctca taaaaatgcg
     1621 gatcggtgcc ggtgccccag ttctcctgaa gcgggcggcg tctctccgga atgctcgtat
     1681 ggttcggttt taggatgata cggtgcgtca ccgggacgcc ggcgcgctcc atcggcacga
     1741 agatctggcg cgcaagcgaa aggccctctg cctgcacggc tgcggcgtcc atcttgtcgg
     1801 caacatgtgt acggcggatg aacaccgcct tcggattgct ctcaataaac gtgtgcagac
     1861 cgaagtatgc ctgcggcgca cggctggctg ccgcattcag ccggaaaccg gcggccgctc
     1921 ctgccgccgc cattgtccca gtgcggataa agtttcgccg gctcagtgtc atgccgatct
     1981 cccctttgct ctacagtcta actgaagacg atcacaccga ggcgcctcag ttccggaacg
     2041 tagacttccg tcgttgtccc ccgcttcacc gccttcagcg gcacgggagc cgcctcggga
     2101 cgcaacagcg tcgccttggc gtagtgtccc tgaatccgcg cgggaaaatc catgtcgaca
     2161 ggcgccccgt agttgatcgc gcacaacacc gccgccccgg gtgcgggtcc ggtcttcgga
     2221 ccggcggtcg cgagcgcgat cacgctgtga gcgttccaga ggcgcaccgc ccgctgtttc
     2281 tgcgtcacga tgtcgatcac gtcgagcgca aactcgctcg gatccgcaat ctgctcgcgg
     2341 tatgcgatga cttgtccccg tccaagcgag aaaaagtcgc ggtcctcctg cgcgcgaacg
     2401 tgcttcagtt ccggcgcccg ccaccacgcc ttttcccccg gtgcgtcaac cacaagacta
     2461 cagccggctt ccgcgagcgc cagcagtttc ctgcgcgcct cgggagtcgg gtcggaaata
     2521 ctagccgcga cgagagccag gcagtgcgga ttcggcggtg gcggcgcggc ggccgaggca
     2581 agcctcggcg agacgttctg gcggtacatc aggttcacca gttcggccgt gatgtcgtcc
     2641 tccacgagag cggttgccac aggcagaacg ggttgcccga aaagcgaccg gtgctcgcgc
     2701 agccacgcgg ccgtccgccc taactggcgc cacgccgttc ctgttttctc atcaccttcc
     2761 aggagcgctt tgcagaacct tggctccacc gccagcagga agttcccgcc gttggcccat
     2821 gcctcgatca gggcgagttc cagcgaatcg tgcggcacga cgcggtccgg tttgagcccg
     2881 gcttcctcat tcggaaggta cccgagaacc gcgggctgct tcgggtacat cgcgcggagg
     2941 tagccgatcc agaatccgtt cgcgtcgatc cacggctgcc ggcttgcgct cgcgacttcg
     3001 tcgttgtggc ccctggcgcc gccgcgactg atgccgggcc acaggccggc cttcaaagcg
     3061 gccggtttca cgggcgcctt gtgcatctcc tcgacgccga gcaatgggat atcggccggg
     3121 tccgccgtct tcaatcctgc ctgccggcac gcttccgcga acgctgcatc gacgcgcgga
     3181 ggcaggaccg cggtggctcc gcattgtttc agcagtggga tgtgtttcgg atcaaggtca
     3241 tcccaacgga tgacaacgtc ggaagagaga tcaagaggca ttgttatgca cccgcgcggc
     3301 cctcggagcc gtgcgggaga tcacccggag aattccaccg tttccggccg ttccgtaaat
     3361 gacgcggtga tgaaatcgcg gttcatccgg gcgatcacct ctagcttaat cgacttcggg
     3421 cacgctgcct cacaggctcc gatgttggtg caactgccga aaagttcctc gcccatggcc
     3481 gcgatcatgc tgagcgctct gcgcttgcgc tccggctgac cttgcggcag caaaccgagc
     3541 tgggagatct tcgcgcccac gaacagcgcc gccgcggcat tcgggcacgc cgcgacgcag
     3601 gcgccgcatc cgatgcaagc cgccgcgtcc atcgctcttt ccgcgatttg cttcgggatc
     3661 ggcaccgcgt tgccgtccgg cgcgctgccc gtcgatacgg atatgtaccc gccggcctgg
     3721 atgatgcggt cgaacgcgct gcgatccacc accaggtctt tgattaccgg aaaggcgcgg
     3781 gcccgccatg gctcgacgta gatcgcgtcg ccgtccttaa agtgccgcat gtgaagctgg
     3841 cacagcgtgg tcccgggctg cggtccgtga gcgacgccgt tgaccatcgc tccgcacatg
     3901 ccgcagatgc cctcgcggca gtcgtgatcg aacgccactg ggtcctcgcc cttcgtcatc
     3961 agctcttcat tcaggacgtc cagcatctcc aggaacgaca tctcggggct gacgtccttt
     4021 aactcgtagc gaaccatgcg tcccggcgcg tgtgcattct tctgccgcca gatgtggaga
     4081 gtgatcttca ttacttgtaa ctccgttgtg acgggatcac gtattcgaat ttcagcggct
     4141 ctttgtgcaa tatgggccgc ctatccgctc cctggtattc ccagaccgcc gcgtgacaat
     4201 agccctcgtc gtcgcgcaac gcctcgccct cgggcgtctg gtactcctcc cggaagtgac
     4261 ctccgcacga ctcgttgcgt tccagcgcat ccaggcatat cagctcgccc agttcgagga
     4321 aatccgcgac ccggcccgcc ttttccagac attgattgaa ctcctcgttc gtgccaagga
     4381 cgttgacgtt cttccagtat tcctcgcgca gcgccggaat tttctccaac gcttgcctga
     4441 gtccggcggc gttccgcgcc atcccgcaat cctcccagag gatcttgccg agttcgcggt
     4501 ggaatgaatc gacggtccgc ttacctttga tcgacagcag ccgattcgtg gccgcaacga
     4561 cgttctcctc ggcttccctg catgctgggt tgtctggccc caccttgtct agcttcgccg
     4621 tggcgaggta gtcgccgatc gtgtagggca gcacgaagta gccatcggcc aggccctgca
     4681 tgagggcgct ggcgcccaaa cggttggccc cgtgatcgga gaaattcgcc tcgccgatca
     4741 cgaacaggcc cgggacgttg ctcatgagct ggtagtccac ccagagcccc cccatcgtgt
     4801 agtgcgtcgc cggaaagatg cgcatcggcg attcgtacgg gttctcggcc gtgatgcgct
     4861 cgtacatctc gaaaagattc ccgtagcgct ccgcgatggt gtgccgtccg agccgctgga
     4921 tagcgtcgga gaagtcgaga tagacaccaa gtccggtctc gccgaccccc cggccgtcgt
     4981 cacacacctg cttggctgct cgcgacgcca cgtcgcgcgg gacaaggttc ccgaaggccg
     5041 ggtaccgccg ctccagatag tagtcgcgct ccgcctcggg gatctcggct gccggccgct
     5101 tgtcgcctgc ctcttttgga acccaaatgc ggccatcgtt gcgcagcgac tcgctcatca
     5161 aggtcagctt cgactgatac tcccccgtca ccggaatgca cgtcgggtgg atctgcgtga
     5221 agcacggatt gccgaatccc gcgccccgct tgtacgacct ccagattgcg gtagcgttcg
     5281 atcccttggc gtacgtcgcc aggaagaacg cattcccata cccgcctgtg ccgagcacaa
     5341 ccgcatccgc gagatggatg tcgatctctc ccgtgatcag gttccgtgtc acgatgcctc
     5401 gtgcccggcc gtcgatcacg atcaaatcca gcatctcggt gcgtgggaac atggtcacgt
     5461 tgcccgctgc tacctggcgc atcagcgcct ggtacgcgcc gagcagcaat tgctggcccg
     5521 tctgcccccg cgcatagaac gtccgcgaga cctgcgcgcc tccgaacgag cggttcgcga
     5581 gcattccgcc gtactcccgc gcgaacggaa caccctgtgc cacgcactgg tcgatgatgt
     5641 tgacgctcac ctgcgccagc cggtacacgt tcgactcgcg ggcgcggaaa tcgccgccct
     5701 ttaccgtgtc gtaaaacaaa cggaagatgc tgtcgccgtc gttctggtag ttcttcgctg
     5761 cgttaatgcc gccctgcgcc gcgatgctgt gcgcccgccg cgggctgtcc tggaagcaga
     5821 aggctttgac gttatacccc agctcgccga gcgtcgccgc ggccgagcct ccggcaagcc
     5881 ccgtgcccac catgatgaca ctgaacttac gcttgttcgc cgggttgact agcttcatct
     5941 cgaagcggta cttgtcccag cgctgctcga tcgggcctga cgggatcctg gcgtccagtt
     6001 tcatgagtgc gctcctgtca tcgaatcaag ccggccatca ccgacaccgg gatcgaaatg
     6061 ttgcccaccg caataaatat ggcgaatccg gctgccagcc gcttcagcag tggcgtgtat
     6121 cggggatggc tgaaaccgac tgtctgaaac atgctccata cgccgtgata caggtgcatg
     6181 caaagaagga ccatcgccac gatgtacgcg atggacaccg ccggaatccg aaacccgttc
     6241 acgacattgt tgtagacgtc cagttcgcgg tacggaaggt tgaccgagcc cgtcgtcaaa
     6301 tgcagaatgt ggaacacaac gaatgccgcg atgatcggcc cgctccacag catggtgcgc
     6361 gatgcgtaac ccgctgggac gttttcctcc ttcgcgtatc ctaccggcct cgcccttttc
     6421 ttcaggatcc agagctggat ggacgtcgtc acgtgcagaa ctacagccgt cagcagcagc
     6481 aagcgtacga cccaaagcag cgcgggtttg gagtggagga attccgcgta tctgttgatc
     6541 tgttccggcc cgaggtagat ctggaggttg cccagaaggt gcccaagcac gtaaccgaaa
     6601 aggatcaccc cggtgacggc catcactact ttcttgccga tcgaggcctg ataaaatcgc
     6661 gtggcctgat ccaggcggac gctcactgcc gaagagctca ttgcacctct ccaaagcggt
     6721 ggttaattac aagaggacta ttgtatgcaa atctctgcga ctcggtggcg cccatccgct
     6781 ctcctcacgg agtcgtctcg ccatgtggcg cggacccctt caagtgccgg tagtagccgg
     6841 gatcctgccc ggtcaactgt ttggtcgcga agatgatctg gccggtgtgc tgcgagaagt
     6901 gctcgacaac atgatagatg gcctccagca cggtcacgtg gtacgcctga atcgtgactt
     6961 tctcggcgag ccgctccggc gtcaccttcg ccaggacatc cagcgctgcc cggatagtgc
     7021 cttccatccg ctcggagagc tccaccggct gtacctcgcc ccgggcggcg aactcccgat
     7081 cgcgcacacg cacatccgct tgtcccccga caccatgtcc gacccactgc cttacgttgc
     7141 cgcataggtg gagcacgaga ttgccgacgg cgttctcgct gtcggcgccc cgcacccaca
     7201 cctgctcata cgtaagcttg tccaggcaat cctggatcct cgaactgagc tgtgtcagct
     7261 tctctgccga atagcggaga aaatctgcgg caagtttccc gtccatggtt cacaacacct
     7321 gttccgacca gttctccagc accgccctca tcttctgcat gaactggtcc gctagcgccc
     7381 catcgatcag cctgtggtcg aacgtcaatc ccatcaggga gatatggcgg atggcgattg
     7441 cgtcgtcctc caccactacg ggcgttttat ccaccgctcc tacgccgagg atagccacct
     7501 gcggctggtt gatgatcggc gtgccaaaca ggctgccgaa gctgccaaaa ttcgtaatcg
     7561 agaacgtgcc gccgtggacc tcgtcgggct tcaactggcg cgagcgcgcc cgcgccgcca
     7621 ggtcaacgat cgcccgctgg agtcccagca cgttcttctc gtcggcgttg cggatcaccg
     7681 gcacgatcag cccgttgtcc agcgccaccg cgatcccgac gtgaatctcg ttgtggtaga
     7741 tgacgttggc gccctcgatc gaagcattga cgatggggaa agcccgcagc gcttcggccg
     7801 ccgcgcgcac gacgaacggc aggaaagtca gcgagaagcc gtagcgctcc ttgacgagtt
     7861 ccttgttgcg ctgccgcact ttggctaccg ccgtcatgtc gatcttgtgg accgtcgtga
     7921 cgtgcgccga agttcgtttg ctcgccacca tgtgctcggc gatcttttgc cgcatgatgc
     7981 tcatcggctc gacgcgcgcc ctcgccggct cgggcctcgg caccggcggc atctcggtgg
     8041 gaactgccgg aggcgggggc atgaccggtg cgggtggcgg cggagccgcg accgtccgag
     8101 ccgcctgggc ggcgatgtag gcctccacgt cctgcttcgt gatccgccct cccgcgcccg
     8161 tgccctttac ctgcctcagg tcgatgttgt actcgcgtgc catcttccgg accagcgggg
     8221 agagaggccc ggcgacctcc tccggtgccg ctgcgggggt aggcgcggct gggggtgg
//
LOCUS       DATIFQ010000014         8296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_578303, whole genome shotgun sequence.
ACCESSION   DATIFQ010000014 DATIFQ010000000
VERSION     DATIFQ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578303"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="VLE22_00490"
     CDS             complement(<1..1930)
                     /locus_tag="VLE22_00490"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HSB12900.1"
                     /translation="MRVDLLFPRLSLKARLISNYLVVLGIGGLVTSLVGSWIVSSTIM
                     MQARRSADLDLAVALTLYDNHLNGLKQAVGLVSTGATIQQHLRSRDRTVLFQYLETVY
                     KTAGFDFLTLADPRGEVLLRVSNRERSGDSVPPLEVVRTALSGSTAAGTEILSPEVLG
                     AENPALPARAHLRLVPTPRTSPSNKTEETSGLVQLVAAPVRGPAGDILGVLYGGVLLN
                     RNFDLVDRAWDLLFQGDRFDGQDVGTVTIFQRDVRVATNVRTDRGERALGTLVSDEVR
                     EAVLGRGERWRDRAFVVNDWYISAYEPIRNPRGEIVGIFYVGLLEKAFTSIRNRVILS
                     FFGLASAGFVLIIAITYYEILKITRPIGEMVASTRNIAAGRFDQEVRTDSVGEVKLLA
                     ASFNTMLESLRQMRNDLEEWGRTLEEKVNKRTEELVAMRARVEQAERLASLGKLAAGV
                     AHEINNPLGGIMALTALSLEDMKPDDPNRENFEEVLRQSERCRDIVRGLLEFSRQSEA
                     HMELVDLNKVLQDTLSLLERQALFLNINVVRNLDASLPPVMADRSQLQQLFMNILMNA
                     VQAMNEKGTVTLSTCRNGSGQAEVAVSDTGSGIPADKIDRIFDPFYTTKPADQGTGLG
                     LSIAYGIVTRHGGTISVES"
     gene            complement(1920..3224)
                     /locus_tag="VLE22_00495"
     CDS             complement(1920..3224)
                     /locus_tag="VLE22_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HSB12901.1"
                     /translation="MSATITVKHLARVEGHGGVTVELDGNAVRDVRFDIFEGARLLEG
                     LVRGRSYQDVSQFLSRICAICSVAHALTSIKATEAAFDVRPSAQTELLRDLLYRGESI
                     ESHALHVFLLALPDYLGYSGALAMAAEKPEAVMLGLRLKKLGNSIQEVIGGRAVHPVN
                     AIVGGFGKLPDSDELIALRRELLLAVGEAESVIRLVASLPPANFCRGQTQYAAMRCPR
                     DYGYYAGDEIEVAWNGTRRTLRANEYRALAGEKAVPHSHAKHSLHDGRPFMVGALARL
                     SVNADKLEGRAAEARERLGLRPPFTNPMENNEAQAVELIFDLEHALRTVDRLLSDGVR
                     PEAPVSVRSKAGSGTAVTEAPRGLLVHSYEYDEEGRVVSADVITPTAFNAASVENHLR
                     LAAEQSAEKEPDALAHKLEMIVRAYDPCISCSVHVVRIGNAS"
     gene            complement(3221..3979)
                     /locus_tag="VLE22_00500"
     CDS             complement(3221..3979)
                     /locus_tag="VLE22_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12902.1"
                     /translation="MAKPKVGIFGLTGCAGDQLVILNCEDQLMDLAGALDIRDFLMAS
                     SDNDTECELDLALVEGTVLTRRDEERLRRIRERAKLLAAIGTCAVWGGVAAETNGFDR
                     TRLMREVYGELGAGYDSTAPRALHDVVKVDLNITGCPIEKEQAVAAVANLLNGNLPAY
                     PDYPVCAECRMRENNCLLIDRNEACCGPLTVAGCNARCPALGVACLGCRGPAADANFP
                     SALAMFAERGIPREHIARRLRMFAPVTLPEGGQA"
     gene            complement(3979..4851)
                     /locus_tag="VLE22_00505"
     CDS             complement(3979..4851)
                     /locus_tag="VLE22_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HSB12903.1"
                     /translation="MLRAPETPVYQPVLTHNPYQPHMARIVRIDKMVEDNFLFTVRFV
                     DQEMAQTFRHRPGQFVMLSVPGTGEAPISISSSPTRPGVLEMCIRRVGRVTNALYRLR
                     TNDLVAVRGPYGNGFPTRELTGDDLLFAAGGLGMAPLRSLLWYALDNRQRFGKITLLC
                     GARTPADMLFRDELLSLVDRADLTCLLTVDSDPAGGWRHHVGRLPRLFDYVKIDPART
                     YAAVCGPPVVYKYVLERLLALGLPKDRILMSLERRMKCGIGKCGHCNVGYKYTCQHGP
                     IFTYWDALNLPEMI"
     gene            complement(4835..5869)
                     /locus_tag="VLE22_00510"
     CDS             complement(4835..5869)
                     /locus_tag="VLE22_00510"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSB12904.1"
                     /translation="MKILKLPKGKLDFFASVVQQFGEVHAPVEENGRYVFRRLARWSE
                     ARLAYDRTTIPPKKYFLPPRETLFRYRTETGYEPYSGDIDKRIVLFGLHACDIYALNL
                     LDKVFAGRYPDPYFQSRRRNISIIGIDCAPDGKCFCRSMRADFVDHGFDLFFTDIGDY
                     YLALVGTARGDDMVLATGPLFEPVSQDDIDLYKQRSLRKRQAFELEVEIRDLPEIFEM
                     EYHNEVWGELGERCLSCGSCSMVCPTCYCYDVRDDVELGSRAGPRTRLWDSCLFLSHA
                     VVAGGENFRKNRASRIKFRYYHKQRGFVAEYGRPSCVGCGRCITACPVGIDITQVIEQ
                     LRGVHNAARA"
     gene            complement(5866..6294)
                     /locus_tag="VLE22_00515"
     CDS             complement(5866..6294)
                     /locus_tag="VLE22_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB12905.1"
                     /translation="MAIKTILMVDDDRDFVEAVSSFLESNGYRVVRAYDGREGLRLAK
                     MEQPDLILMDIVMSERTEGFFAVQELRRVPGLSAVPIFVVSSLYQTLTDFRIPPGSEW
                     LSHDEFFAKPVDLPAFLDKIRGRIGDGAGNRSTNTGASPP"
     gene            complement(6284..7453)
                     /locus_tag="VLE22_00520"
     CDS             complement(6284..7453)
                     /locus_tag="VLE22_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB12906.1"
                     /translation="MPSLTPEVSEPTGQETIVVVDDDHAIRLSCQKILAKAGFRVETF
                     ENGARGLEGVTELRPALVIVDLKMPGLSGIEVIARVREICPQTIVIVITGYATIDTAV
                     EAMKSGAYDFLPKPFSPEALRMIVNRGLERRNLLLLTERSELEREMLKRRFVTFVSHQ
                     LQTPLVAVHQYLDVINQLDDTQQTAAKKREWIDRCLRRTEEMQAIIRDWLTLAKLEGG
                     TLSRVKVKVDLERIVRDIFATYEQLAAAEEITLEARFPEGGCFVPGDHNCLNVLFDNL
                     IVNAIKYNKRGGRVTVSAELADGEVAVSVADTGVGIPERCRPMLFREFYRIEREGCKK
                     VSGTGLGLLISKRIVTEMGGTISLESEVDAGSTFRVRLPEWHEDAGEAAGRANGD"
     gene            complement(7482..7886)
                     /locus_tag="VLE22_00525"
     CDS             complement(7482..7886)
                     /locus_tag="VLE22_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB12907.1"
                     /translation="MATGARVLMIDDDADFRESVRTVLESNGYEVFEAATGKEGLQKL
                     VEHKPGLIILDIMMECCTEGYGVNQAIKYQDAFEGYRNIPIVMVSSIEQSPDERFPMA
                     GELDMIRPDLYMTKPLDIPRFLEVVRKTIRTS"
BASE COUNT         1525 a         2646 c         2514 g         1611 t
ORIGIN      
        1 cgctctcgac cgagatcgtg ccgccgtgcc gggtcacgat cccgtaagcg atggaaagcc
       61 ccagacccgt tccctggtca gccggcttag tggtgtaaaa cgggtcgaag atgcggtcga
      121 tcttgtcggc cggaattccg ctaccggtat cggagacagc gacttccgcc tgccctgatc
      181 cgttccggca agtgctaagt gtgaccgtcc ccttttcatt catcgcctgc acggcattca
      241 tgaggatgtt catgaaaagt tgctgaagct gcgaccggtc ggccatcacc ggaggtaagg
      301 aggcatccag gttccgaacg acgttgatgt tcaagaacag cgcctgcctt tcgagcagcg
      361 aaagcgtgtc ctggaggacc ttgttcaggt ccaccaactc catgtgcgcc tcggattgcc
      421 gcgagaactc gagcagtccg cgcacgatgt cccggcagcg ttcggattgc cgcagcactt
      481 cctcgaagtt ttcccgattg gggtcgtccg gtttcatgtc ctcgaggctc aacgccgtca
      541 gtgccatgat gccgccgagc gggttgttga tctcgtgggc cacgcccgcc gccagttttc
      601 caagtgaggc gagccgctcc gcctgctcga cgcgcgcgcg cattgcgacc agctcttcgg
      661 tgcgcttgtt gactttctcc tcgagcgtgc gtccccattc ttcgaggtcg ttccgcatct
      721 gccgcagact ctcgagcatc gtgttaaacg atgccgcgag tagcttcact tcgccgaccg
      781 agtccgtcct gacctcctga tcgaagcgac ccgccgcgat gtttcgggtt gacgcaacca
      841 tctcgccgat cggccgcgtg atcttgagga tctcgtagta tgtgatggcg atgatgagca
      901 cgaagccggc cgatgcgagg ccgaagaagg aaaggattac gcggtttcgg atggacgtga
      961 acgccttctc caggagcccg acgtagaata tgcctacgat ctcgccgcga ggattccgaa
     1021 tcggctcata agcgctgatg taccagtcgt tcacgacaaa cgcgcggtct cgccagcgtt
     1081 cgccccggcc gagcacagct tcccggacct catcggagac aagagtgccc agagcccgct
     1141 ctccgcggtc agttcgcacg ttggtcgcga cgcggacgtc cctctggaaa atggtcaccg
     1201 tgccgacgtc ctgtccgtcg aagcggtctc cttggaaaag caggtcccag gcgcgatcca
     1261 ccagatcgaa attgcgattc agtagcactc cgccgtacag cacgccgagg atgtcacccg
     1321 ccgggcctcg gacgggtgcc gccacgagtt gaacgaggcc ggaagtctct tcagtcttgt
     1381 tcgaaggact ggtcctcggt gtcggcacaa gtcggagatg cgcccgtgca ggaagcgccg
     1441 ggttctcggc tcccagcact tccgggctga ggatttcggt accggcggcc gtggatcctg
     1501 agagtgccgt gcgaacaact tccagtggcg gaacgctgtc gcccgatcgc tctctgttgg
     1561 agacgcgcag caagacctca ccacggggat cagccagtgt gaggaaatcg aatccggcgg
     1621 tcttgtacac cgtctcgagg tactgaaaca acaccgtacg gtcgcgcgac cgtaggtgtt
     1681 gctgaatcgt ggcacctgtg gagaccaggc ccaccgcctg cttcaagccg ttgagatggt
     1741 tgtcgtacag cgttagcgcg acggcgaggt ccagatccgc cgaccgccga gcctgcatca
     1801 tgatcgtcga gctgacgatc cacgaaccca ccagcgaggt gaccaaccct ccgatcccga
     1861 gaactacgag gtagttcgaa atcagtctcg ctttaagcga gagccgcggg aataggaggt
     1921 caactcgcat tgccaattcg aacaacgtga acggagcacg aaatgcaagg gtcgtacgcg
     1981 cggacgatca tctcgagctt atgtgccagc gcgtccggct ctttctctgc gctctgttcg
     2041 gccgcgaggc gcagatggtt ctcaacgctg gcggcgttga aggctgtcgg cgtaatcacg
     2101 tccgcgctca cgacacgccc ctcttcatcg tattcgtagc tgtgtacgag cagtccgcgg
     2161 ggcgcctccg tgacggcggt gccgctgccg gccttggacc gcacggagac gggagcttct
     2221 gggcgtactc cgtccgagag cagccggtcc acggtccgaa gggcgtgctc gagatcgaag
     2281 atcagctcga ccgcctgcgc ctcgttgttc tccatcgggt tcgtgaacgg gggccgaagt
     2341 cccagacgct cccgtgcctc ggcggcccgg ccttcgagtt tatccgcgtt caccgagagc
     2401 cgcgcaagtg ctccgaccat gaacggacgg ccgtcgtgca ggctgtgctt cgcatgggag
     2461 tggggaacgg ccttctcccc cgccagcgcc ctgtattcat tcgcgcggag cgtcctgcgg
     2521 gttccgttcc aggctacttc gatctcgtcg ccggcatagt agccgtagtc gcgcgggcag
     2581 cgcattgcgg cgtactgcgt ctgtccccgg cagaagttcg ccggcggcag cgaggccaca
     2641 agtcggatta ccgattcggc ctctcccacg gccagcaaca gctcgcggcg cagcgcgatc
     2701 agttcatcgg aatccggcag tttcccaaag cccccgacga ttgcgttcac cgggtggact
     2761 gcccggccgc cgatcacctc ctggatggag ttcccaagtt tcttcaaccg aagcccaagc
     2821 atcacggctt ccggtttctc ggccgccatc gccagtgctc cgctgtagcc cagatagtcg
     2881 ggcaatgcga gcaggaacac gtgaagcgcg tgactctcga tgctctcgcc gcggtacaac
     2941 agatcgcgca gaagctccgt ctgcgcgctc ggccggacat cgaaagccgc ctcggtcgcc
     3001 ttgatcgacg tgagcgcatg ggccaccgag cagatggcgc agatgcgcga aaggaactgc
     3061 gagacgtcct gatagcttct tccgcgaacg aggccctcga gcagacgtgc gccctcgaaa
     3121 atgtcgaaac gaacgtcgcg gacggcgttg ccgtcgagtt ccacggtgac gccgccgtgt
     3181 ccctccacgc gggcgagatg cttgactgtg atcgttgcgc tcatgcctgg cctccctccg
     3241 gcagcgtgac cggtgcaaac atccgaagtc gtcgtgcgat atgctcccgc gggatgcccc
     3301 tttccgcgaa catcgcaagc gcggacggaa agtttgcatc tgcggccggc ccccggcaac
     3361 ccaggcaggc cacgccaagg gcagggcacc gggcgttgca gcctgcgacg gttagaggtc
     3421 cgcagcaggc ctcgtttcgg tcgatgagca ggcagttgtt ttcccgcatg cggcattcgg
     3481 cgcaaaccgg gtagtccggg taagccggca agttgccgtt cagaagattc gcgaccgccg
     3541 cgacagcttg ctccttctcg atcgggcagc cggtgatgtt caagtccacc ttcacaacgt
     3601 catggagcgc gcgcggggcc gttgaatcgt agcccgcgcc gagttcgccg tagacctcgc
     3661 gcatcagccg cgtgcggtca aatccgttgg tttccgcggc cacacctccc cagacggcgc
     3721 acgttccgat cgccgccagc agtttcgcgc gttcgcggat ccggcgaagg cgttcttcgt
     3781 cccgccgggt cagcaccgtg ccctccacca gcgccaggtc gagttcacat tccgtgtcgt
     3841 tgtcggacga ggccatcagg aagtcgcgga tgtcgagcgc gccggcaagg tccatcaact
     3901 ggtcctcgca attcaggatc acaagctggt cgccggcgca gccggtgagt ccgaagatgc
     3961 ccacttttgg tttcgccatc agatcatctc cgggaggttg agcgcgtccc aatacgtgaa
     4021 gatgggcccg tgctggcagg tgtacttata gccaacgttg caatgaccgc acttcccgat
     4081 gccacacttc atgcgccgtt caagcgacat caggatccgg tccttcggaa gaccgagggc
     4141 aagcagtcgt tccaggacgt atttgtagac tacgggcggc ccgcacacgg cggcataggt
     4201 ccgtgccgga tcgatcttta cgtagtcgaa gagcctggga agccgtccga catggtgcct
     4261 ccagccgccg gccggatcgc tgtcgaccgt cagcaggcac gtgaggtcgg cacggtctac
     4321 caaggaaagg agttcatccc gaaaaagcat gtcagccggc gtgcgcgctc cgcacagcag
     4381 tgtgatcttg ccgaatctct gcctgttgtc cagcgcgtac cagagcaggg aacgcaacgg
     4441 cgccatgcct aggccgccgg cggcgaacag caggtcatcc ccggtgagct cgcgggtggg
     4501 aaacccgttg ccgtaaggtc cgcgcaccgc gacaaggtcg ttcgtcctga ggcgatacaa
     4561 agcgttggtc acgcgcccca cacggcggat gcacatctcg agcacgccgg gccgggtcgg
     4621 ggatgaggag atcgagatcg gcgcttcacc ggtacctggc acgctgagca tcacgaactg
     4681 gccgggtcgg tgccggaacg tctgggccat ctcctggtcg acgaatcgca ccgtgaagag
     4741 gaagttgtcc tctaccattt tgtcgatccg cacaatccgg gccatgtgcg gctggtacgg
     4801 gttgtgagtc agcacaggct ggtatacggg tgtttcaggc gcgcgcagca ttgtgcaccc
     4861 ccctcaactg ctcgatgacc tgagtgatgt cgattccgac ggggcacgcg gtgatgcagc
     4921 gcccgcaacc cacgcagctc ggccggccgt actcggcgac gaagccgcgc tgcttgtggt
     4981 agtaccggaa tttgatgcgg ctcgcccggt tcttccggaa gttctcaccg ccggccacga
     5041 cagcatgact caggaatagg caggaatccc acaaccgggt tcgcggtccc gcgcgtgaac
     5101 ccagctccac atcgtctctg acgtcgtagc agtaacatgt cggacaaacc atgctgcagc
     5161 ttccgcacga caggcagcgc tcgcccagtt cgccccagac ctcgttgtgg tactccatct
     5221 cgaagatctc gggcagatca cggatctcga cctcgagttc gaatgcttgc cgcttgcgca
     5281 gggagcgctg cttgtacagg tcgatgtcgt cctgcgagac cggttcgaac agcggacccg
     5341 tggccagcac catgtcgtcg ccacgcgccg ttcccaccag cgccaggtag tagtcgccga
     5401 tgtcggtgaa gaagagatcg aacccgtgat ctacgaagtc ggcgcgcatg gagcggcaga
     5461 agcactttcc atccggagcg cagtcgatgc cgatgatgga gatgttgcgc cggcgggact
     5521 ggaagtacgg gtccgggtaa cgcccggcaa agactttgtc caggaggttc agcgcgtaga
     5581 tgtcgcaggc gtgcaggccg aaaagaacaa tgcgtttatc gatgtctccc gaatacggtt
     5641 cgtagccggt ctcagttcgg taacgaaaca aggtttccct gggaggcagg aagtacttct
     5701 tcggcgggat ggtcgttcgg tcgtaggcga ggcgtgcctc ggaccaacgc gctagccgcc
     5761 ggaaaacgta gcgcccgttc tcttccaccg gcgcgtggac ctcgccgaac tgctgaacga
     5821 ccgacgcgaa gaagtcgagc tttcccttcg gcagtttcag aattttcatg gtggtgacgc
     5881 tccagtgttt gtgctcctgt tcccagcgcc atcgccgatg cgcccgcgaa tcttatctag
     5941 aaacgcgggc agatcgaccg gctttgcaaa aaactcgtca tgcgagagcc actcgcttcc
     6001 ggggggaatg cggaagtccg tcagggtctg atagagggaa ctgacgacaa agatcgggac
     6061 ggcgctcaga ccgggcacgc gccgcagctc ctggaccgcg aagaagccct ccgttctctc
     6121 gctcatgacg atatccatga gaatgagatc aggctgctcc atctttgcca gccgcaagcc
     6181 ctcccggccg tcgtacgctc ttacgacacg gtatccgttg gactcgagaa aggacgaaac
     6241 ggcctcgaca aagtcgcggt catcgtccac catcaggatc gttttaatcg ccattcgctc
     6301 tacccgctgc ttccccggcg tcttcatgcc attccggcaa ccggacccgg aaggttgatc
     6361 cggcatcgac ctcgctttcc aagctgatgg tgccgcccat ctccgtgaca atgcgtttcg
     6421 agatcaacag ccccagcccc gtccctgaaa ccttcttgca cccctcccgc tcgatacggt
     6481 agaactcgcg gaacaacatc ggccggcagc gctccgggat tcccacgccc gtgtcggcaa
     6541 ccgagactgc cacttcgccg tcagccagtt ccgcggagac ggtgacccgg ccgccgcgct
     6601 tgttgtactt gatcgcattc acgatgaggt tgtcgaacag tacgttcaga cagttgtggt
     6661 cgcccggtac gaaacagcct ccttccggaa accgcgcctc gagggtgatc tcttcggccg
     6721 cggccagttg ctcgtaggtg gcgaagatat cgcggacaat tcgttcaagg tcaactttca
     6781 ccttcacccg ggagagagtg ccgccttcca gcttcgccag cgtcagccag tcccggataa
     6841 tggcctgcat ctcctcggtc cgccgcaggc agcggtcgat ccattcccgt ttcttcgcgg
     6901 cagtctgctg ggtatcgtcc agttggttga tgacgtccag gtactggtgg accgcgacaa
     6961 gcggcgtctg caactggtgg gagacaaagg tcacgaaccg ccgcttcagc atctcccgct
     7021 ccaactcgct ccgctcggtg agaagaagca ggttgcggcg ctcgagcccg cggttaacga
     7081 tcatccggag cgcctcgggc gagaagggtt tcggcaggaa atcgtaagcg cccgacttca
     7141 tcgcttcaac cgccgtgtcg atcgtcgcgt atccggtgat cacgatgacg atcgtctgcg
     7201 ggcagatctc gcggacccgg gcaatcacct cgatcccgct tagaccgggc atcttcaggt
     7261 cgacgataac gagcgcggga cgcagctccg tgacgccttc cagccctcgg gcgccgtttt
     7321 cgaaggtttc cacgcggaag ccggccttgg ccaggatttt ctggcaggag aggcggatgg
     7381 cgtggtcgtc gtccacgacc acgatggttt cctgcccggt cggctcactc acctccggag
     7441 tgagactggg cacgcatatg ctggcccggt tcggttccaa gtcaggaggt ccggattgtc
     7501 tttcgcacga cctccaagaa cctcgggatg tcgagcggtt ttgtcatgta caggtcggga
     7561 cggatcatat ccaattcgcc cgccattggg aaccgttcgt ccgggctctg ctcaatggat
     7621 gagaccatga cgatcgggat gttccggtac ccttcgaacg cgtcctgata cttgatggcc
     7681 tggttgacgc cgtagccttc cgtgcagcat tccatcatga tgtctagaat gatgaggccc
     7741 ggcttgtgtt caaccagctt ctgaaggccc tccttgccgg tcgcggcttc gaagacctcg
     7801 taaccgttgc tttccagaac cgtgcgcacg gactcccgga agtccgcatc gtcgtcgatc
     7861 atgagtactc ttgcgcctgt ggccatgtaa cctcctcgcc gcacccgcgg cgatttcacg
     7921 gcccagatgc ccgtagccgc ctcatttaca cctattaagg acctgaattt ctgattcaga
     7981 cccctgcgat gaatatacga cttcccgccc ccgggtgcaa gcccggctcg tgcttccggc
     8041 agccggcctt gcggacaccg gagggcggac gggaacggaa cttgctccgg acctgtccat
     8101 gcccagggcc tgccatagcc gcttcgccgc ttcagtgatg tgcgggcagc gcgatgaact
     8161 ccacaccgtt cgcgcagcgg cggcatcgcg cgcgacgcgc tcttctctca ccggaaattc
     8221 tccaccagca tcaggtcgct gccggattgg tcattctggc cgtacaggat gaagcgttca
     8281 tcgggggaca cagaca
//
LOCUS       DATIFQ010000015         4910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_387589, whole genome shotgun sequence.
ACCESSION   DATIFQ010000015 DATIFQ010000000
VERSION     DATIFQ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387589"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VLE22_00530"
     CDS             <1..322
                     /locus_tag="VLE22_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12908.1"
                     /translation="FLYYMKTDDEDSALWRVPAEGGEETQVLESVSCTNFAVVEQGIY
                     FIARMSEPVVQFFEFATKKVVTIARLQRTAAYGLDVSRDGRSLVYSEYGPWQGDLMLV
                     ENFR"
     gene            319..2448
                     /locus_tag="VLE22_00535"
     CDS             319..2448
                     /locus_tag="VLE22_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB12909.1"
                     /translation="MSATEPSSTVRFGLFEADLAAGELRKRGRKIELQDQPFHVLALL
                     LGRPQEVVTREELRQALWPADTFVEFDESLNKAVQKLRQALGDSADNPRFIETLPRKG
                     YRFIAPVDAGAVQTVVPIRPHRLLLAGVASVLVLAGVVAVWLSQRSGKTAAEPPMPIP
                     LTTDPGDEWNPSFSPDGNQVAFARYGDIHHGDIFLGQGDIYIKLIGASEPLRLTTDPA
                     NDYGPAWSPDGRSIAFLRDLTAERSGVFLMPAIGGGERKVAEVHRLDVWGPHLSWHPG
                     GRWVVVSDKNSAGDPQALFLLSVETGEKRRLTLPPRDGTEDYNPAVSPDGRRVMFARC
                     KAGLGDLYALELSDDLSPRGEPKRLTFENRYTAGPAWWPDGRSVVFISSASVHITSLW
                     RMALRGFGGRPDKPERLAFAGEGAREAAVSRQGRLVYANLVIDADIWRMELKHGPSRT
                     PTAGSNVKLISSTRLDHTPAYSQDGRRIAFASNRSGSHEIWVCSSDGSHAVQVSSFGG
                     PYIADPRWSPDGEWIIFGGSHLIRSDGGPTERLTGDVQGWSRDGRWLYIISNRSGEPQ
                     IWKTPWPLTGNNRQPRQITKKGGGYFVRESLDGRFVYYLKGDLPSFALSSLWKVPVDG
                     GEESRVIGAVYADNYAVADHGIYFIPNPQNAALHFLDLASGKVAKVASLDTYPAYGFS
                     VSPDGRWLLYTQYAGQGSDLMLVENFR"
     gene            2489..2565
                     /locus_tag="VLE22_00540"
     tRNA            2489..2565
                     /locus_tag="VLE22_00540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2523..2525,aa:Arg,seq:cct)
     gene            2695..>4910
                     /gene="dnaE"
                     /locus_tag="VLE22_00545"
     CDS             2695..>4910
                     /gene="dnaE"
                     /locus_tag="VLE22_00545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSB12910.1"
                     /translation="MSDTPFVHLHCHTDYSLLDGACEIGQLMQLAEEQGMPAVAITDH
                     GNLFGAAQFYNEAKSRGIQPVIGCEVYVSQQGYKTRSDTDRYNHLVLLCESQEGYRNL
                     IKLVSTGFLEGFYYKPRVDKDLLGSHSKGLIALSACLRGDINEKLMADQYEEAKRAAY
                     GYEDIFGKGNFFLELQDHGLDGDRKVLPQINRLSQETGVPLVVTNDSHYLRKDDARAH
                     EILLCIQTGKTVNDASRMKFWTPDFYLKSRAEMLRLFAEVEDGLDRTWEIAQRCHVKL
                     EKIAEPFPKFEVPPEHTTDTYFEFVARHGFEKRRVRLEAMRATDRLKHDLPEYLERLD
                     REIRMIQQMKFSGYFLIVWDFIRFAKSQGIPVGPGRGSAADSLVSYAMEITDIDPLQY
                     GLLFERFLNPERISLPDIDIDFCMNRRGEVIQYVTEKYGREQVAQIITFNTLGARAGI
                     KDVGRVLDMSYADVDRLTKMIPGVLNITLDHAMKMEPGLGEAAKKDQRVAEVLEVARR
                     IEGMCRNASVHAAGVVISPQPLQELVPLYRTNKDEIVTQYDMNSLEKLSLLKMDFLGL
                     TTLTIIHDALGLIEKNHGVKLELEDLPLSDPKTYEIFSKGYTSGVFQFESNGMRDILR
                     RYQPTRIEDLIALNALYRPGPIQGGMIDDFIDRKHGRKEISYDLPELKEILEETYGVI
                     VYQEQVMQISNRLAGYSLGEADILRRAMGKKKPEEMAKQRERFLHGAKEKGFPHKKV"
BASE COUNT          987 a         1378 c         1649 g          896 t
ORIGIN      
        1 gtttctttac tacatgaaga ctgacgatga ggactcggcg ctttggaggg tcccggcgga
       61 gggcggggag gagacgcagg tgctggaatc ggtcagctgc accaatttcg cggttgtgga
      121 acaggggatc tatttcatcg cacgaatgag cgagcctgtc gtccagttct tcgagttcgc
      181 cacgaaaaaa gtcgtcacga tcgcccggct ccagcgcacc gcggcgtatg gcctcgacgt
      241 ctctcgtgac ggccgatcgc tcgtgtactc ggaatacggg ccgtggcagg gagacctgat
      301 gctggtggag aattttcggt gagtgccacg gaaccaagtt cgactgtgcg cttcggattg
      361 ttcgaagcgg acctcgctgc gggcgagttg cggaagcggg ggcggaagat tgagcttcag
      421 gatcaaccct ttcacgtcct ggccttgctg ctggggcgac ctcaagaggt agtcacccgt
      481 gaggaattgc gtcaggccct ctggccagcc gacaccttcg tcgagttcga cgagagcctg
      541 aacaaggccg tccagaagct gcggcaagcg ctgggggatt cggcggacaa cccgcgattc
      601 atcgagacgc tgccgcggaa ggggtaccgg ttcattgcgc cggtggatgc gggtgctgtg
      661 cagacggtgg ttccgatccg gccgcaccgt ctgctgttgg cgggggtggc gagcgttcta
      721 gtgctggccg gcgtcgtcgc cgtctggctg agccagcggt ccggcaagac agcggcggag
      781 ccgccgatgc ccattcctct cacgaccgat ccgggcgacg agtggaaccc gagcttctct
      841 ccggatggca accaggtggc gttcgcacga tacggtgata tccaccacgg agacattttt
      901 ctggggcaag gggacatata catcaaactg atcggtgcta gcgagccgtt gcggttgacc
      961 acggatccgg ccaatgacta cgggccggcg tggtcgccgg acggcaggtc gattgccttc
     1021 cttcgggatt tgacggcgga gaggagtggc gtattcctca tgccggcaat cggaggaggg
     1081 gagcgcaaag tggccgaggt tcaccggctg gacgtctggg ggccgcatct ttcgtggcat
     1141 cccggcggcc ggtgggtcgt ggtgtccgac aagaactccg cgggcgaccc tcaggccttg
     1201 tttcttctct cggtggaaac cggggaaaag cggcggttaa cgctgcctcc tcgggatggt
     1261 acagaggatt acaaccctgc cgtgtctcct gatggtcgcc gggtgatgtt cgcacgatgc
     1321 aaagcgggac tgggtgatct ttacgcgctg gaactctccg atgacctgag ccccaggggg
     1381 gagccgaaac gtctcacatt tgagaaccgg tatactgccg gcccagcctg gtggccggac
     1441 ggacgctcgg tcgtcttcat ttcctcagct tcggtccaca tcaccagcct atggaggatg
     1501 gccctgcgcg gtttcggagg acggccggac aagccggagc ggctggcgtt cgccggcgaa
     1561 ggcgctcggg aggcggcggt ctcgcgtcag ggccggctgg tatacgcgaa tctcgtcatt
     1621 gatgcggaca tctggcgcat ggaactgaag catggaccga gcagaacacc aacggccgga
     1681 tcaaacgtaa agctgatttc ttccacacgc ctcgaccaca cgccagcgta ttcgcaggat
     1741 ggccggcgca tcgcgttcgc ctcgaaccgc tcgggcagtc acgagatctg ggtgtgcagc
     1801 agcgatggat cgcacgcggt gcaggtgtcg tccttcggcg gcccatatat tgccgacccg
     1861 cggtggtcac ccgatggcga atggatcatc ttcggtggct cgcatctgat tcgctccgat
     1921 gggggaccga cggagcgtct taccggtgat gtgcaggggt ggtccaggga cggcaggtgg
     1981 ctgtacatca tctcaaaccg ctcgggggag ccgcagatct ggaaaactcc atggccactc
     2041 actgggaaca atcgacagcc caggcagata acgaagaagg gcggcggcta tttcgtacgg
     2101 gagtctctag acggcaggtt tgtctactac ttgaagggag atctccccag ctttgctctg
     2161 agcagtctgt ggaaggtacc ggtggatggc ggtgaagagt cccgggtgat cggggcggtt
     2221 tacgccgata attacgctgt tgccgatcat ggcatctact tcatcccgaa tccgcagaat
     2281 gctgcgctgc atttcctgga tctcgccagc ggcaaagtgg cgaaagtagc ctcgctcgat
     2341 acgtatccag catatggctt ctcggtgtct ccggacggcc ggtggctgtt gtacacgcag
     2401 tacgccgggc agggtagtga tttgatgctg gtggagaatt ttcggtagtc gtgcgccggc
     2461 ggccggctga tacaattgaa agtggcctgg ggacgtagct cagatggata gagcggccgc
     2521 ctcctaagcg gcaggtcggc agttcgaatc tgcccgtccc taccatgcgg aagatggagt
     2581 aggggacagg cagctctgtc ggttcgcagg ccacaaacga cgatggcctg cgccactgga
     2641 tagagaagcc tgtcctggta tttcgaggtc ctcccccggg aacggtagcg cggaatgtcg
     2701 gataccccat tcgttcacct gcactgtcac acagactact cgctgctcga cggagcctgc
     2761 gagatcggcc agttgatgca actggccgag gaacagggga tgccggcggt cgcgatcacc
     2821 gatcacggca acctcttcgg ggcggcgcag ttctacaacg aagcgaagtc gcgtgggatc
     2881 cagccggtga tcggctgcga ggtgtacgtc tcgcagcagg gctacaaaac ccgttctgac
     2941 accgaccgct acaaccactt ggtgctgttg tgcgaaagcc aggagggcta ccgcaacctg
     3001 atcaagctgg tgtcgacggg gttcctcgaa gggttctact acaagccccg agtcgacaag
     3061 gacctgctgg gcagccactc gaagggcttg atcgcgctgt cggcgtgcct gcgaggcgac
     3121 atcaacgaga agctgatggc cgaccagtac gaggaagcga agcgggcggc ttatggctac
     3181 gaagatatct tcgggaaggg gaacttcttt ctggaattgc aggaccacgg cctcgacgga
     3241 gaccggaagg tgcttccgca gatcaaccgc ctgtctcagg agacgggcgt cccgctggtg
     3301 gtgacgaacg actcgcacta cctgaggaag gacgacgcgc gcgcccacga aatcctgctc
     3361 tgcatccaga ccggcaagac ggtcaacgac gcctcgcgaa tgaagttctg gacgcccgat
     3421 ttctacctga agtcgcgcgc ggagatgctg cgactgttcg cggaggtcga ggacgggctt
     3481 gaccggacgt gggagatcgc gcagcgctgc cacgtcaagc tcgaaaagat cgctgagccg
     3541 ttcccgaagt tcgaagtgcc gcccgagcac acgacagata cgtacttcga attcgtggcg
     3601 cggcacggtt tcgagaagcg ccgcgtgcgc ctcgaagcga tgcgggccac ggaccggctg
     3661 aagcacgacc tgcccgaata tctggagcgg ctggaccgcg aaatccggat gatccagcag
     3721 atgaaattct ccggctactt tctgatcgtc tgggatttca tccgcttcgc gaagtcgcag
     3781 ggaatcccag tcgggccggg acgcggatcg gcggcggaca gcctggtgag ctacgcgatg
     3841 gaaatcacgg acatcgaccc gcttcaatac gggctgctgt tcgaacggtt cctgaacccg
     3901 gagcgcatca gtctgccgga tatcgacatc gacttctgta tgaaccgtcg cggcgaggtg
     3961 atccaatatg tcaccgagaa gtacgggcgc gagcaggtgg cacagatcat cacattcaac
     4021 acgctcggcg cgcgggcggg gatcaaggac gtcggccgcg tgctcgatat gagttacgcg
     4081 gacgtggacc ggctgaccaa gatgatcccc ggcgtgctga acatcacgct cgaccacgcg
     4141 atgaagatgg agccgggcct aggcgaggcg gcgaagaagg accagcgcgt ggcggaggtg
     4201 ctggaggtgg cgcggaggat cgaggggatg tgccgcaacg cctcagtgca cgcggccggg
     4261 gtggtgatct caccgcagcc gctgcaggaa ctggtgccgc tgtaccgcac gaacaaggac
     4321 gaaatcgtca cgcagtacga catgaacagc ctggagaagc tgtcgcttct caagatggac
     4381 ttcctcgggc tgacgacgct gacgatcatc cacgatgcgc tggggctgat cgagaaaaac
     4441 catggcgtga agctggagct cgaagacctg ccgctgagcg accccaagac gtacgagatc
     4501 ttctcgaagg gctacacgag cggcgtgttc caatttgagt cgaacgggat gcgggacatt
     4561 ctgcggcgct accagccgac ccgcatcgag gacctgatcg cgctgaacgc cttgtatcgt
     4621 ccggggccaa tccagggggg gatgatcgac gattttatcg accgcaagca cggccgcaag
     4681 gagatcagct acgacctgcc ggagctgaag gagatcctgg aagagacgta cggggtgatc
     4741 gtctaccagg agcaggtcat gcagatctcc aaccggctgg ccggctactc gctgggcgag
     4801 gcagacatcc ttcgccgggc gatggggaag aaaaagcccg aggagatggc caagcagcgg
     4861 gaaaggttcc tgcacggagc gaaggaaaag gggttcccgc ataagaaagt
//
LOCUS       DATIFQ010000016         5031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2111888, whole genome shotgun sequence.
ACCESSION   DATIFQ010000016 DATIFQ010000000
VERSION     DATIFQ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2111888"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VLE22_00550"
     CDS             <1..684
                     /locus_tag="VLE22_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12911.1"
                     /translation="ERRLFDGDYFFSTMPVQELVRALDRPAPPEVVEVSEGLMYRDFI
                     TVGLLVGGLKVKEDHPDGSKLIRDNWIYIQEPDVLVGRLQIFNNWSPYLVPDRTKVWL
                     GLEYFCNESDPLWTKSDAEMTRLAAEELARIGIIDAGDVLDATVVHVPKTYPAYFGTY
                     PRFHVVREYLDRFENLFLIGRNGMHRYNNQDHSMLAAMVAVDNIAAGVPGKDNIWAVN
                     TEQEYHEEK"
     gene            707..3481
                     /locus_tag="VLE22_00555"
     CDS             707..3481
                     /locus_tag="VLE22_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB12912.1"
                     /translation="MTERLNDDWLRFSGGLRARGDRLCQELTSAWDDLRALIASVAAA
                     SDSVSRTPPDAVRVQSLEALFNEASERLLFTPLAEHQRKRPLHRALSAIEDYEAALEV
                     LISGLPEQVEAKSRELARTIDPDGRSGLRRRWLEWRNASLALPLRDIVSGFLLREQLW
                     RSRIEGNFLVALSQATLLLTTRWQDFRRWQLYVLSGRELVPATSDEERKWWHELRSQV
                     ETRAVAALGAERNSLAAEAVRLPAVVLAAELLPASRRTGMLERRRAHLAYWSRLTRGV
                     QSLLDLNRQMSTLERDATMEAGQIVDSLGSERAGSLGELDAAVGWLEAWERGERDSAY
                     PAPVTGLHSAEERAADWTHRLTRLARLHMPEALETVVPRRPLPGLRAPWRLITPQRTV
                     ITAIEQAGRPLLAASLRETETSHRAIVREIERAREVVAFGLETASTGGADLASEAVHN
                     ALSLLRHHRESASDAVPWAEGRLTRAHAVVFLESHVALEQGWLGFLEYLTRYRGRMRI
                     EELSGRLAGHVQTSALRLSRTARHFVDWVLLKTGWIAPPTPRVEPVIERPYLGQVLDV
                     RIGARDLPMLYLRLFRLAPVEDPRFLIGREAEMSGLADALARWESGRKVSVLIVGARG
                     TGKTSLLNCAAASVLSEFPVVRGQFCRRISRTQELFAFLRGLLRLPDDADVEAVLSSG
                     RRVVILEELERIFLRNINGFEALHAFLRLIESSSGSTLWILSTNEWSFRYLDAVAGLS
                     RPFTHRVNAMTVSPEHLTGAVLQRHNLSGLRLQFEPVPEGDPRVSRVRRLLGLERDAQ
                     QLFFDSLFRQSEGVFRSAFELWQDSIERVEAGAVLMRHPLAPDYRPLMRELNLDDLFT
                     LQVVLQHGGLDSSDTAGVLGIPLAVAARRIERLRALELLEPEPDCPGFRVRPQARRFV
                     RDALGRHNLF"
     gene            3493..4329
                     /locus_tag="VLE22_00560"
     CDS             3493..4329
                     /locus_tag="VLE22_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HSB12913.1"
                     /translation="MPVSLFSFAMLLQTARPGEIFRYFTLGRFLSVAFVLLLTWVLIR
                     YLSRLLAFLSAQGAKTRFLVKWIEPVLRIALWFVAIFVSFGVLAPTQETFLAAVGSLA
                     IAIGLGAQDLVKNLIGGLVILADRPYQLGDRVRIGDAYGEIDHIGLRSTKLTTLDDTR
                     VTIPNANILDMAIFNANSGVPDCQVVTDLFLPCDTDPDLAVRIGYETAWTSPFVYLAK
                     PVAVLLIDEYSETPYLRLRVKAYVFDHRYENRMKNDITVRAKKEFSRLGLLAAWRAPA
                     EA"
     gene            4343..>5031
                     /locus_tag="VLE22_00565"
     CDS             4343..>5031
                     /locus_tag="VLE22_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12914.1"
                     /translation="MRDAPAFYNQAVLRVLPLFFTAACCVAQPLRGPIVAIDFYGAAA
                     VDFTRLRAAFPFQVGDIFQTEATDQDQSPEEFQKLINKNRISVAPVFIPELKGWVLYV
                     DVEPPDASPAIWNPAPAETDKLPAKILHLYEHAMNRFVNGGIHAGDETTNGYSLSKDP
                     VMRADELKLIEYARAHASGIYSVLKRSGSTRDRIAAAWIAGYAPKGLDQLTALLHSVR
                     DPDSTVRNNAIR"
BASE COUNT          852 a         1675 c         1475 g         1029 t
ORIGIN      
        1 gagcgccggc tcttcgacgg agactacttt ttctcgacga tgcccgtcca ggagctggtt
       61 cgcgcactgg atcggcccgc gcctccggaa gtcgtcgagg tcagcgaggg cctgatgtac
      121 cgcgacttca tcactgtcgg cctgctcgtc ggtggcctca aggtcaagga ggatcacccg
      181 gacgggtcaa aactcatccg cgataactgg atctacattc aggaacccga cgttctcgtc
      241 ggccgccttc agatctttaa caactggagt ccctacctcg tccccgacag aaccaaggtc
      301 tggctcggcc ttgagtactt ctgcaatgag tccgacccgc tctggaccaa atccgacgcc
      361 gagatgacgc gcctcgccgc cgaagaactc gcccgtatcg gaattatcga tgccggcgat
      421 gtccttgacg cgaccgtcgt tcacgtcccg aagacctacc cggcctactt cggcacttac
      481 ccgcgctttc acgtggttcg ggaatacctc gaccgcttcg aaaacctctt cctcatcggc
      541 cggaatggca tgcaccggta caacaatcaa gaccactcga tgctggcggc catggtcgcc
      601 gtagacaaca ttgcggccgg tgtgccaggg aaggacaata tctgggcggt caacacggag
      661 caggagtatc acgaagagaa ataggatcgg tccctgaagg acgggcatga cggagcggct
      721 aaacgatgac tggcttcgct tttccggcgg gctccgcgcg cggggcgacc gtctttgtca
      781 ggagcttacc tcggcatggg acgatctccg cgcgctaatc gcttcggttg ccgcggcgtc
      841 tgactccgtt tctcggacgc cgcccgatgc ggtccgtgtg cagtctcttg aggcgctctt
      901 taatgaagca tccgagcgcc tgttgtttac gccgttggct gagcaccagc gtaagcgccc
      961 gctccaccgg gcgctgtcgg caatcgagga ctacgaggct gcgctcgaag tcctgatcag
     1021 cggcctcccg gagcaggtgg aggcaaagag ccgggaattg gcccgcacca tcgacccgga
     1081 cggacgctcc ggcctccggc gccgctggct tgaatggcgg aacgcttctc ttgccctgcc
     1141 tctgcgtgac atcgtctccg gtttcttgct gcgcgagcag ttgtggcgat cccggatcga
     1201 gggcaacttc cttgtcgcgt tatcgcaagc aacactgctt ctgacgacgc gctggcagga
     1261 cttccgccgc tggcaattgt acgtgctttc cggacgtgaa cttgtgcccg ccaccagcga
     1321 tgaggagcgg aaatggtggc atgagctccg ctcccaagtc gagacgcgcg ctgtcgccgc
     1381 gctcggcgcg gaacggaact ccttggcggc cgaagccgtg cgcctccccg cagtcgtttt
     1441 ggcggcagaa ctcttaccgg caagccgccg gaccggcatg cttgaacggc gccgtgccca
     1501 ccttgcctat tggtcccgtc tgacccgtgg cgttcaatcg ttactcgatc tgaatcgcca
     1561 gatgtccacg ctggagcgcg acgccaccat ggaagccggc cagatcgtag actcgcttgg
     1621 ctccgagcgg gctggctcgc tcggtgaact cgatgcggcc gtcgggtggc ttgaggcctg
     1681 ggagcgcggc gagcgcgaca gtgcgtaccc cgctcccgtc accggcctgc attccgccga
     1741 agaacgcgcc gctgattgga ctcaccgcct gacgcgtctg gcccggcttc acatgccgga
     1801 ggcgctggag accgtggtgc cccgccggcc ccttcccggt cttcgtgctc catggcggct
     1861 gatcacgccg caacgcacgg tcatcaccgc gatcgagcaa gccggccgac cccttctcgc
     1921 cgcgagcctg cgtgagacgg aaacctccca tcgcgccatc gttcgcgaga tcgagcgtgc
     1981 tcgagaagtt gtagcgttcg gattggagac cgcgtcgaca ggcggcgcgg acctcgcctc
     2041 cgaggccgta cacaacgcgt tgtccttgct ccgccaccac cgggagagcg cgagcgacgc
     2101 cgtgccgtgg gccgaaggcc ggctcacgcg ggcccacgcc gtcgtctttc tggaatctca
     2161 cgttgcgctc gagcagggct ggcttggttt cctggagtac ctgacacgct accgcggccg
     2221 gatgaggatc gaggagttgt ccggccggct cgccgggcac gttcagactt cagccttgcg
     2281 cctctcgcgg accgccaggc acttcgttga ttgggttctc ttgaagaccg gctggatcgc
     2341 accgccgacg ccgcgagtcg aacccgttat cgagcggccg tacctcggcc aggtgctgga
     2401 cgtaaggatc ggagcccgcg atctcccgat gctctatctc cggctcttcc gtctggcccc
     2461 agtcgaggac cccagatttc tcattggacg tgaagcggaa atgagcggct tggcggacgc
     2521 cctcgcccgt tgggagtccg gtcgaaaggt ctccgtgctg attgtcggcg ctcggggtac
     2581 cggcaagacc agtctgctca actgtgcggc cgctagtgtt ctttcggagt tccccgtggt
     2641 ccgcggccag ttctgccgcc ggatctcccg gacccaagag ctgttcgcgt tcctgcgcgg
     2701 gctgctccgg cttccggacg acgctgacgt cgaagcagtc ctttccagcg ggcggagagt
     2761 cgtcatcctg gaggaactgg aacgcatatt tcttcggaac atcaacggct tcgaagcatt
     2821 gcacgctttc ctgcggctaa tcgagtccag ctccgggtcc accctttgga ttcttagcac
     2881 caatgagtgg tctttccgct atctggacgc tgtggcaggg ctgtcccgtc ccttcaccca
     2941 ccgcgtcaac gccatgacgg tctccccgga gcacctgacc ggcgccgtcc tccagcgcca
     3001 caatctttcc gggctgaggc tgcaattcga acccgttccc gagggtgacc cgcgggtcag
     3061 tcgagtccgg cgcctcctcg gcctcgagcg agacgctcaa cagctctttt tcgactcttt
     3121 gttccgccag tcggaaggtg tattccggtc tgcctttgaa ctctggcagg attccatcga
     3181 gcgagtggaa gcaggcgccg tcttgatgcg tcacccgctt gctcccgact acaggccgct
     3241 gatgcgggaa ctcaatctgg acgacctgtt cactctccag gtcgtccttc agcacggagg
     3301 gcttgattct tcggatacag ccggcgtcct cggcatcccc ctggcggttg ccgcccgccg
     3361 gatcgagcgg ttgcgtgcac tcgagttact tgagcccgag ccggactgcc ccggcttccg
     3421 cgttcggccc caagcccggc gcttcgttcg cgacgcactg ggccggcata acctcttctg
     3481 aggagttgag cgatgccagt ctcgttgttt tcatttgcga tgctcctcca gaccgcccgt
     3541 cccggtgaga tattccgcta cttcaccctc ggacggttcc tgtccgttgc cttcgtcctg
     3601 ctgctcacgt gggtgctgat ccgctatctg tcgcggctgc tcgctttcct cagcgcccag
     3661 ggcgccaaga cccggttcct cgtcaagtgg atcgagccgg tgctccgtat tgccctgtgg
     3721 tttgtagcaa tcttcgtctc cttcggcgtg ctggccccaa cccaggagac gtttctggcc
     3781 gcggtcggct cccttgcgat cgccatcggt ctgggcgcgc aagatctcgt caagaacctc
     3841 atcggcggcc tcgtgattct cgccgatcgc ccctatcagc tcggcgaccg ggtcagaatt
     3901 ggcgacgcct acggtgaaat cgaccacatc ggcctccgca gcacgaagct caccacgctg
     3961 gatgacactc gcgtcaccat tcccaacgcc aacatcctgg acatggcgat cttcaacgcc
     4021 aactcgggcg tgccggactg ccaggtcgtt acggatctgt tcctgccttg cgataccgac
     4081 cctgacctcg ccgtccggat cggctatgag actgcctgga ccagcccctt tgtctacctc
     4141 gcgaagcccg tcgccgtgct tctgatcgac gaatactccg agactccgta tctgcggcta
     4201 cgagtcaagg cgtatgtctt tgaccaccgt tacgaaaacc ggatgaagaa cgacatcact
     4261 gtccgcgcca agaaggagtt ttcccgcctg gggcttctcg ccgcctggcg cgcgcccgcc
     4321 gaggcgtgag gtttcggtaa tcgtgcgtga cgcccccgca ttctataatc aggcggtgct
     4381 tcgggtcttg cccctcttct tcaccgccgc ctgttgcgtc gcacaaccgc tccgcggccc
     4441 cattgtggcc atcgatttct atggagctgc ggccgtcgat ttcacgcgtc tacgtgcagc
     4501 ctttccattc caagtcggcg atatctttca aacggaggct acagatcagg accagtcacc
     4561 agaggagttt caaaaactca tcaacaagaa tcgcatctca gtagcgccgg ttttcattcc
     4621 cgaactcaag ggctgggtct tatacgttga tgtagagcca ccggacgcat cgccggcaat
     4681 ctggaatccc gcaccggccg aaaccgacaa acttcctgca aaaattcttc atctgtacga
     4741 gcacgcaatg aaccgattcg tcaatggagg gatccacgcc ggggacgaga caacgaacgg
     4801 ctactcgctc tccaaggatc cagtcatgcg tgcagacgaa ctcaagctga tcgagtacgc
     4861 acgagcgcat gcgagcggta tctactcggt cctgaaacgg tccggctcaa cccgggatcg
     4921 aattgcggcg gcgtggatcg ctggctacgc tcccaagggt ttggatcagc tcacagcgct
     4981 tctccactcc gttagggatc ccgattcgac agtccgcaac aacgcgatcc g
//
LOCUS       DATIFQ010000017        21231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1537744, whole genome shotgun sequence.
ACCESSION   DATIFQ010000017 DATIFQ010000000
VERSION     DATIFQ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 21231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21231
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537744"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..413
                     /locus_tag="VLE22_00570"
     CDS             117..413
                     /locus_tag="VLE22_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HSB12915.1"
                     /translation="MALIEQLTAIAGVLALLAATLWWLRRGGFARFQSGGPRRAALGQ
                     LEQLVRLPLTPQHSLHLIRLAGRALLIGRSPAGLTVLESVEWKTGSVTPTEPPE"
     gene            410..1144
                     /gene="fliP"
                     /locus_tag="VLE22_00575"
     CDS             410..1144
                     /gene="fliP"
                     /locus_tag="VLE22_00575"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HSB12916.1"
                     /translation="MRILFRLLPAAVFLPAALAAQASLPVPFGPSGRAEAALSLPMQI
                     VFLLTLLTLLPAAVMSVTPFLRITVVLHFLRQALGAQTVPSNQVLLGLALFLSILIMQ
                     PVAAEMYEKAWKPLEKNQITVTQALERGSRPLRTFLLRFAREKDIRLFVEITRAPAPR
                     NAADLDLRILIPAYILSELRAGFQIGAVLFLPFLVIDLVVASVVLSAGMIQLPPVMIS
                     APFKILLFVLVDGWNLVVGSLVKSFY"
     gene            1160..1429
                     /locus_tag="VLE22_00580"
     CDS             1160..1429
                     /locus_tag="VLE22_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HSB12917.1"
                     /translation="MSFELVVDVLRQSLWTTFWLGAPLLLVGFIAGIGVSLLQIVTSI
                     QDSAFGTVPRLAAFLAGLVLLMPWMLKKLAAYTVAVFGDFGRYAG"
     gene            1419..2186
                     /locus_tag="VLE22_00585"
     CDS             1419..2186
                     /locus_tag="VLE22_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HSB12918.1"
                     /translation="MPASLAIPPSLLFGFLLVLARVAGALVFVPIPGVQRGPEMARAV
                     ISVAVTLALFSLWPVAVDAPDPGRLAGWLLFEAALGVMVGLAVSFLTETLVMAAQVVG
                     LPAGFAYASMVDPQTQADSGVLLVFAQLSAGLLFFAAGLDREVLRIFARSLESLPPGA
                     VALPASAGEALIRLGAGIFTVGFRLALPAVALLMLVDIALALVGRVHAQLQLLTISFP
                     VKMLAAVAVLAWTLTLFPRLYVGYAGRAFGVLRELIR"
     gene            2220..3284
                     /locus_tag="VLE22_00590"
     CDS             2220..3284
                     /locus_tag="VLE22_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HSB12919.1"
                     /translation="MADQGQRTEQPTPRRLEKARREGQFASSRELLGAAQFLVFVAVL
                     AAWGPDWLSAMSEAARLLLSQAFRADLDHQRLLRLLAETLSRVFAPLLLGGALIMAAG
                     LAAQLAMTRLGFSLEKTAPDIRRLNPASRLKEMFRQNVASFVKSLVLLPVFAAAVWAI
                     AAANLPSYRSLPLDSIEAGMREVAGSILDLLWKAAGAFVILGSIDLFRQHRRYLRDLR
                     MSKHEIREEAKEMEGNPQMKMRIRRLQRDLLRRQMMRQVKTATAVIVNPTHFAVAIRY
                     QSEQMAAPKVVAKGKNYLAQRIRALAVENQIPLVENPPLAQALYKAVDVGQEIPAHLY
                     RAVAEVLAYILRLMNGRLQG"
     gene            3297..5378
                     /gene="flhA"
                     /locus_tag="VLE22_00595"
     CDS             3297..5378
                     /gene="flhA"
                     /locus_tag="VLE22_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HSB12920.1"
                     /translation="MAETKAMAWLGGWKLPQGVSLTALGELGVPLAVLAIVLALIIPL
                     PTFLMDLLIVTDIMMSVVVMMVSMYIRRPVEFSVFPTTLLLLTLFRLALNISSSRLIL
                     LNGNHGTGAAGDVIEAFGSFVVGGNYIIGAVIFLVLIAIQYVVINHGAVRISEVTARF
                     TLDALPGKQMSIDSDLNAGIIDEAEARDRRRQLVGEAEFYGAMDGASRFTQRDAVASI
                     LITGINIVAGFLIGILQHGMDFRRALETYTVLTIGDGLVTVIPALMISISGGLIVTRA
                     SSEARLGAEFRKQVFGNTEPLMLASAVLVALAAFPGLPKIPFLLMGVGLGVSAWRRKK
                     RSIESEKTAAAAKPALARENLEALLRVEPIAVEVGLGLVQLVEGGQNSLLLRRIGGIR
                     RQLAAELGYILPPVRVTDNLSLGAREYVVLLKGERIAKYELPYGRELAIPLGKPDPSL
                     EGQPTREPAFGMNALWIPSERAERARSLGYTVVDAVSVAGTHLAEIVRRHACELFSRQ
                     DAKKLLDRVAAENPRVVEDLVPKLLPLATVQRVLQNLLRERVAIRDAVSILESLSEAA
                     AVSRNPVLLTEYVRQSVRRAVIKPYLNATGELPAYFFDPSIEQVIESSIEHGEHHSHL
                     AAQPQVLREILTRIQNRIGTPGAPVAAVANSGARYFLRQVLESSLPGLFVLSHNEIPP
                     GVKVVSQGVIG"
     gene            5583..6353
                     /locus_tag="VLE22_00600"
     CDS             5583..6353
                     /locus_tag="VLE22_00600"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HSB12921.1"
                     /translation="MTVSAGEVVYSGSHTEGTVAERERLILDHLPQVRLIARRIHERL
                     PATVSLDDLVSTGILGLISAIDNFDPGQNVKLKTYAEYKIRGAILDSLRDLDWAPRDK
                     RRKAKQIEAAISAAEQRLQRVATEEELAAELSLEVEEYREWLVEIQGLSLNSYEFGFG
                     GKDERNLLDIVPAGDEHSPSQVFELAELERLLAGAIERMPAVERTVLSLYYQEELTLR
                     EIAKVMNLHLSRISQLKSQAILRLRSFLGKRWPTRRGR"
     gene            6359..6745
                     /gene="fliN"
                     /locus_tag="VLE22_00605"
     CDS             6359..6745
                     /gene="fliN"
                     /locus_tag="VLE22_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HSB12922.1"
                     /translation="MNPSDLLEALGKPGASDPARQGDDAPASGLNSKTLDLLLDVELP
                     VTVSFGRALLPLQDVLKLSAGSIVELNRTISEPVELIVNNCVIALGEVVVVEGNYGVR
                     IRQIASRENRLSTSSDSLLPAQFPRG"
     gene            complement(6695..8050)
                     /locus_tag="VLE22_00610"
     CDS             complement(6695..8050)
                     /locus_tag="VLE22_00610"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB12923.1"
                     /translation="MLAALEGVDVDVFPDIAPALSHLRSSGADAVLVTLPIPGWTAEE
                     TLEEIQRASASAPVLIREDSGAFDRAVRLARLGALCYFGEPLCAADVARRIEAAVEER
                     RSTDLASLSAALGREPWRRWLVGASRAVGSVIETIRLVGPRRSTVLITGETGTGKEMA
                     ARALHLASPRGHLPMVTVNCSALPEHLLEAELFGHVKGAFTGAVQSRIGRFEQADGST
                     LFLDEIGDMPLELQAKLLRVLQEREFQRLGSSETVRVDVRVAAASNSDLPEKVRRGQF
                     REDLYYRINVVPIEMPPLRERLVDVPLLVLHFIEKICRIENIPAKQVGGETLERLSSY
                     SWPGNVRQLENAVEMAVALSGQRPVLYPSDFPLPSPVREQPSRCESAPSITVPDHGLD
                     FDEVVGCFERSILDQAMRKSGGNKKRAADLLRLKRTTLSAKLRSTSGKLGREERIAAC
                     R"
     gene            8283..8618
                     /locus_tag="VLE22_00615"
     CDS             8283..8618
                     /locus_tag="VLE22_00615"
                     /inference="COORDINATES: protein motif:HMM:NF016223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HSB12924.1"
                     /translation="MRVDDRNLTPGAASQTGKTAATQDVDRVPDSRSTGTRETGEGDE
                     VELSGLVARLSRAISAASTDRTDRIEELSNRFEQGRYQVDSLAVGRAILSELRAAGHE
                     RPDGAPAAL"
     gene            8646..9017
                     /locus_tag="VLE22_00620"
     CDS             8646..9017
                     /locus_tag="VLE22_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12925.1"
                     /translation="MKSTDTIDSLGRVRSGVRWALAQTATPSSEILDRCLPVLKAAEA
                     DLRDSAPPLAGAASSKNKREMLRQLRELKKDLGSLATMLDHAAGFYDGCKRLHEPQDD
                     GYDARGAPQPSCPASRVSVEG"
     gene            9054..10493
                     /gene="flgK"
                     /locus_tag="VLE22_00625"
     CDS             9054..10493
                     /gene="flgK"
                     /locus_tag="VLE22_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HSB12926.1"
                     /translation="MPNLLNSLLSSANALRVFERVLVVSQNNVANASTPGYAAQRLSL
                     QAVDFQPELGLMGGVRAGHVESARSLYAEQAVRRQCESLGFFSQAAQSLASIESAFDI
                     SGQRGISGALNRLFESFSAWSLTPNSANARASVIESAGQLSDAFHETASMLAGATIDA
                     DRQIRETIAQINRIGARLQEYNLEGRRGAIEEAGLDTRIQAALEELSELVNFDAIFQA
                     DGTVTVLLGGETPLVVGANRYEVSASFFTPPNPPPVYAAATPSAHVLDANGRDITYQI
                     DQGKLGGLLNVRNEVLPSLRGDGQQPGELNQLAMSIADRVNAILTSGRVSDGPPPENG
                     VPLFTYDAGNQTLAAQSLAVRPGITAEQLAAIDPGPPYVSNGIALRLAALASPRDPSD
                     KIDGFSYTEFYGGIAGRVGQALSEAREGQEFKAQMVSQARYLRNEISGVSLNEEAMRI
                     TEFQRAYQANARMVTLLDELTETAVNLLR"
     gene            10516..11436
                     /locus_tag="VLE22_00630"
     CDS             10516..11436
                     /locus_tag="VLE22_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12927.1"
                     /translation="MIRNLDPSAERFLLDLRRIQTVTGHAQREISSGLRVGSPSDAPD
                     QIGDILQLRAGILRAAQIRMNLGRLQTETAAAEDALNLSVKVMDRARVLGSRGAGSTE
                     TAEARQILAAEVEALLEQLVSASRTTVENRYVFSGDRDREPAYELDLAVPGGVDRKFT
                     TQATRRVEHPNGSSFEVSRTAQEIFDLRLLDDTPAPENAFTALNNLRVALQNNDQAAI
                     DTALPAMRLAGDYLNHMLSFYGSVQHKVDEAFDSAKRLETQLTIELGEKRDADVASAI
                     VELQQSVAQEEAALGARAQLPRTSLFDYLG"
     gene            complement(11474..12838)
                     /gene="accC"
                     /locus_tag="VLE22_00635"
     CDS             complement(11474..12838)
                     /gene="accC"
                     /locus_tag="VLE22_00635"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HSB12928.1"
                     /translation="MFKKVLIANRGEIALRVICACKELGVRTVAVYSEADRNSLHVRF
                     ADEAVCIGPAKSALSYLDIPSVISAAEITNVDAIHPGYGFLSENADFAEVCELSNIRF
                     IGPRPEVTRLMGVKDKARAAMAAAGVPVLPGSDSVLKSPEEAEKVAGQIGYPVILKAS
                     AGGGGRGMRIVREPAELAGLFAQAQQEAGAAFSSPDVYLEKYVEAPRHIEFQVLADQY
                     GNVEVFPERECSIQRRHQKLVEESPSPKVTAELRARVAASLREALHAVGYTNAGTVEF
                     LMDENGELYFIEVNARIQVEHPVTEFITGVDLVKGQILAASGMPLSEIVTEPVRIRGH
                     AIECRINAENPETFAPSPGRITALNIPGGMGVRVDTAAYSDCVIPPYYDSLVAKLITH
                     GRDRAEAIARMHRALEMFVVEGISTSISLHQQIMADPDFQAGNFDTNFISRFLAARET
                     ASKR"
     gene            complement(12863..13363)
                     /gene="accB"
                     /locus_tag="VLE22_00640"
     CDS             complement(12863..13363)
                     /gene="accB"
                     /locus_tag="VLE22_00640"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HSB12929.1"
                     /translation="MTIDEIRELIKVVTETGVAELEVQRGDNRVRILRNAPVASQQHI
                     VVPAITAAMPAPIPAANPYPDATPATAPAPATDEAEAETDDNSLIVVKSPIVGTYYEA
                     PSPGAPPFVQTGDIVHPGQVLCIIESMKLMNEIEAEIASVVVKRLVANAQPVEYGEAL
                     FAVRPL"
     gene            complement(13393..14466)
                     /locus_tag="VLE22_00645"
     CDS             complement(13393..14466)
                     /locus_tag="VLE22_00645"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HSB12930.1"
                     /translation="MISTEFISRRRLVTADLARRRLDLFLISNLVNVRYLTGFTGSNA
                     LLLLSPDGAVLYTDGRYRIQATEETECTVKVVQGSLIPRVAKSLPRGRLGFEAAHLTF
                     DAYRTLKAHLPSKVAAMPVDGLVEEYRMVKSEAEIETMRRSAALACNAFEQTLQHVRP
                     GIRELDLAAELDYRMRRLGAESPAFETIVASGERSALPHASPTAKVLGNQEILLIDMG
                     AFLEGYASDMTRTIFLGAARPKAKRFHHAVLEAQQAALEAVREGTAAKSVDRAARRVL
                     RTAGLERAFVHSTGHGLGLEIHESPRVARKVELQLKAGMAITVEPGVYLQGFGGIRIE
                     DMVVVNRGGCEILTPASKELIEL"
     gene            14565..15815
                     /locus_tag="VLE22_00650"
     CDS             14565..15815
                     /locus_tag="VLE22_00650"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB12931.1"
                     /translation="MRRRDALKAGLAAAPLFVPSHVLGGAGRAGANDRIQIGFIGLGG
                     RARWLIEHEEFPGADIVAATDCFPPQYETAAKIKPSGEKWRKYSDYRRMLDKEKLDAV
                     FVETTTHARVLIMIHALQAGFNIYGEKPLTLTIAEGRTLVNAVKRYNRVLQTGTQQRS
                     IPVNAWASKMIREGILGKVQTVITCNFEPPVGWYPKPAQKIPEGLDWDQWCNQTELRP
                     YHSQLRRNWALWWDYDGAGQSWGVTGWGTHSLDQVQCALGTDDTGPVELWPESESFTA
                     AVTARYANGTLLKMEGPKRDHADLGAIFIGEKGKIEIKRGTCVADPPDLLKDAPPDTP
                     EGPGENRYHIGNFLDCIRTGKLPNAHAEVGHRSTTLCHLVNMCRDLGRKLQWDPKAER
                     FIGDEQANSLLSRPRRKGYELPTI"
     gene            complement(15823..17277)
                     /locus_tag="VLE22_00655"
     CDS             complement(15823..17277)
                     /locus_tag="VLE22_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSB12932.1"
                     /translation="MKFGIECTVPVFLAVQIAMAQPMRVLDLDVGETDEVSQVKLLEH
                     SAVADNVRDAVRVASVRVKIKQEEIRLVCGAYRLPVTVAGVQIDCAVTKDLLRNSRTN
                     PWGLRKDARLRLWPAGSPLLAPGTYTYPVKQRWFATDTQMANEPSFVDGSEEPSGRKI
                     YYHYGLDIGGAEGLTEVVSATDGRVVVRGKTALPEYVKSPYTEVSYDGVIVLDDRGWF
                     HWYFHLASIDTAVELGKRVRIGQRIGLIGKEGSAGCWSHLHYEIRGPQPSGEAGIVEG
                     YAFLREAYWNQYHPKIVAVARPHSFAWVGEPVTLDGSRSWVDSGQIVRYQWRFSDGTS
                     ASGPTITRKYRKPGTYSEILQVTDSRGLIAYDFAVVQTVERQSTKPGTEERVPPTIHA
                     SSWPALNVRAGDPVTFLVRTCRTTFGHETWDFGDGSAPVTVTSDGCAEEKAKEGYART
                     QHVFKKPGDYIVRVERANERGEKATAHLWVPVLK"
     gene            complement(17290..18258)
                     /locus_tag="VLE22_00660"
     CDS             complement(17290..18258)
                     /locus_tag="VLE22_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HSB12933.1"
                     /translation="MKAILVYEPGGPEALTLADIPVPQPGPKEALVKIAAIGVNFIDI
                     YFRSGLYKADLPISIGQEAAGTVEAVGAEVTEFAPGDRVAYAMARGSYAEYQSVPAAM
                     LVKLPDTVPFESAAAAMLQGMTAHYLTHSTFPLKKGDTALVHAAAGGTGLLIVQMAKM
                     RGARVIGTTSTEAKAQLALAAGADEVILYTKQDFEAEARRLTGGRGVDVVYDSVGAST
                     FEKSLDCLRPRGMMVTFGNASGAVPATQPLALSQKGSLFLTRPTLMHYTLERKELLWR
                     ANDVLGWVGSGKLKVRVHQTYPLAEAAQAQIDLAGRGTSGKLLLKP"
     gene            18406..19233
                     /locus_tag="VLE22_00665"
     CDS             18406..19233
                     /locus_tag="VLE22_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSB12934.1"
                     /translation="MRFVALLLLILADASGQAPDWLRLGLLTAMPVKWDVEANWNTFQ
                     RTLEAHASDGIDLAVTPECFLDGYAVADKEWTRERFEAIAQHPEKSPYLARLRDLARK
                     HRVFILFGYTENRGGRFFNTALLMDRDGRTVGRYDKTHLQDHDLRFSPGEDLPVFETP
                     WGKLGVLICADRRWPETSRVLRLRGALLVLIPSYGMWHEANEWWMRTRSYENENFLAF
                     AHPNVAFVANPRGEILAKLQTNLPAMLVCDLNLTEVTGDKHLRDRRPELYRDLAAPK"
     gene            19285..20964
                     /locus_tag="VLE22_00670"
     CDS             19285..20964
                     /locus_tag="VLE22_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB12935.1"
                     /translation="MTVGKAASAFFLFVLAGVWTVPLSAAGEQAWAVPTFESLGLYYN
                     RPIAKDPCRAWYRVAGTQEWREGQALVYDEREKQYRGSLVGLTANTPYEIRLEADGQK
                     IEFETRTRSETFPVGKTTYLPGDGTDQTLYIRDGGTADAWHVVTPRPGTKFVSDVFNL
                     SDYNVVVEADYVILRGLELKNAGMHGVLIRAGVQHVVVEDCHITGWGRLGGARVWGVL
                     FGSDSAVYAEADAGHLVIQRNLLEYPRGGANDWESGHPSGPQAITLTDSRGGNVIRYN
                     TIRSTDDHGFNDGIGGSSNFSFKGSPNRDSDIYGNVISNCWDDAIESEGANMNVRIWS
                     NYIHHTFVHIATAATSMGPLYIFRNVFGESRISHQDSSGGMMIKTGMNYLNIAGERVS
                     TGLGYRFIYHNTALQPNGGLDVFSSHELHNAVSRNNIFYARGRAYPADRGEPRNDFQN
                     DLTGSYLGGGIVRSMFLPSEGLEWYPAPTASRIQWGRVEYTRNGRNFAITDPLVQAKN
                     PALDGGVRLPGFNDEFQGAAPDIGAFEAGLPPLHFGREMAPGFTRPPWELY"
     gene            complement(21036..>21231)
                     /locus_tag="VLE22_00675"
     CDS             complement(21036..>21231)
                     /locus_tag="VLE22_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12936.1"
                     /translation="ATLTSGPSNSSGVAEGTWQTKAPNKRGIGGTNPGPYTATVTGVT
                     AAGYQWDGIQVHVDFTLAPK"
BASE COUNT         3755 a         6865 c         6647 g         3964 t
ORIGIN      
        1 cgcgctgccg gggcgttgat cggctacgcc gagatcgtgg taacggaaag cgtcgcggcg
       61 gtgcggatta cggatttcaa ggccgaagag tgaccgccgc gaggcggtga agggggatgg
      121 cgctgatcga acaacttacg gcaatcgccg gcgtgctggc gctgctggcc gccacgctct
      181 ggtggctacg ccgcgggggg ttcgcgcgtt tccagagcgg cggaccgcgc cgggcggccc
      241 ttgggcaact ggagcaactg gtgcggcttc cgctgacgcc ccaacattcg ctccatctga
      301 tccgcctggc gggccgcgcg ctgttgatcg ggcgctctcc ggcgggtctg accgtccttg
      361 aaagcgtcga atggaagacc ggaagcgtca cgcctacgga gcccccggaa tgagaattct
      421 gttccgcctg cttccagcgg ctgtgtttct gccggccgcg ctggcggcgc aggcgtctct
      481 gccggttcca tttggtccct cgggcagggc tgaggccgca ctcagcctgc cgatgcagat
      541 cgttttcctg ctgacgctgc tgacattgct gccggcggcg gtgatgtcgg tgacgccgtt
      601 cctgcgcatc accgtggtgc tgcattttct ccgccaggcg ttgggggcgc agacggttcc
      661 ctccaaccag gtgctcctcg gtctggcgct gttcctgagc attctgatca tgcagccggt
      721 tgccgccgag atgtacgaaa aggcgtggaa accgctcgag aagaaccaga ttaccgttac
      781 ccaggctctc gagcggggct cacggccact gaggactttt ctgctccgtt tcgccaggga
      841 aaaggacatc cgtctgttcg tggagatcac gcgggcgccg gcgccgcgaa acgccgccga
      901 cctggatcta cggattctga ttcccgccta catcctgtcg gaactgcggg cggggtttca
      961 gatcggcgcc gtcctgttcc tgccgttcct ggtgatcgac ctggtggtgg cctcggtggt
     1021 tctctcggcc ggcatgatcc agttgccgcc ggtcatgatc tcggccccgt tcaagatcct
     1081 gctgttcgta ctggtggacg gctggaacct cgtggtcggc tcgctggtga aaagtttcta
     1141 ctgaacactt ggtttcgtca tgtccttcga actcgtagtc gatgttttgc gacagtcgct
     1201 gtggaccacg ttctggctcg gggcgccgct actgctggtg ggcttcatcg cgggaatcgg
     1261 ggtcagcctc ctgcagatcg tcacctcgat ccaggattcg gcgttcggca cggtgcctcg
     1321 actggccgcg tttctggcgg gcttggtact gctgatgccg tggatgttga aaaaactggc
     1381 agcctacacg gtggcagtct tcggggactt cgggcgctat gccggctagc ctcgccatcc
     1441 ccccgtctct gctgttcggg tttctgctgg tgctggcgcg ggtggccgga gccctcgtct
     1501 ttgtgccgat tccgggtgtg cagcggggcc cggagatggc gcgggcagtg atatcggtgg
     1561 cggtgacgct ggccctcttt tcgctttggc cggtggcggt ggacgcgccg gacccgggcc
     1621 ggctcgccgg ctggctactg ttcgaagcgg ctctgggtgt gatggtggga ctggcggtct
     1681 cattcctgac cgaaacgctg gtgatggcgg cccaggtggt tgggctgccg gccgggttcg
     1741 cctacgcctc gatggtcgac ccgcagactc aggccgactc gggcgttctg ctcgttttcg
     1801 cgcagctttc ggccggtctc ctgttcttcg ccgccggcct ggaccgcgag gttctgcgaa
     1861 tcttcgcacg cagcctggaa tcgcttccgc caggggccgt ggcactgccc gcttcggccg
     1921 gcgaggcgct gatccggctt ggcgcgggga ttttcacggt cgggttccgg ctggctctgc
     1981 cggcggtagc gctactgatg ctggttgata tcgcgctggc gctagttggc agagtccatg
     2041 cgcagctcca gcttctcacc atctcgttcc cagtgaagat gctggcggca gtggcggtgc
     2101 tggcttggac gttgacgcta tttccccggc tatacgttgg ctacgccggc cgcgccttcg
     2161 gcgtcttgcg ggaactcatc cggtagcgtc cctgcgccgg ggcggaacga actctcagga
     2221 tggcggatca aggacagaga accgaacagc ccacgccccg gcggctcgag aaggcgcgcc
     2281 gggagggaca gttcgcgtct tcgagagaac tcctcggggc ggcccagttt ctcgtgttcg
     2341 tagcggtgct ggccgcctgg ggcccggact ggctatcggc gatgtcggag gcggcgcggc
     2401 tcctgctttc gcaggccttc cgcgccgacc ttgatcacca gcgcttgctc cgtctcctcg
     2461 cggaaaccct ctcccgcgtc ttcgcgccgc tgcttctcgg tggtgcgctg ataatggctg
     2521 cgggtctcgc cgcgcaactg gcgatgacgc ggctgggttt cagcctggag aagaccgccc
     2581 cggacatccg gcggctcaac ccggccagcc ggttgaagga gatgttccgc cagaacgtcg
     2641 cctcgttcgt aaagtcgctg gtgctgctgc cggtcttcgc ggcggccgtg tgggcgatcg
     2701 ccgccgcgaa cctgccgtcc taccggagtc ttccactgga cagcatcgag gccggcatgc
     2761 gcgaagtggc cggctcgatc ctcgatctgc tctggaaggc ggcgggcgcg ttcgtgattc
     2821 tgggctcgat cgacctgttc cggcagcacc gccgctacct gcgggacctg cggatgagca
     2881 agcacgaaat tcgcgaggag gccaaggaga tggagggaaa cccgcaaatg aagatgcgca
     2941 tccgccgtct ccagcgcgac ctgctgcgcc ggcagatgat gcggcaggtg aagacggcga
     3001 cggcggtgat cgtcaatccc acccacttcg cggtagccat ccgttaccaa tcggagcaga
     3061 tggctgctcc caaggttgtg gcgaagggca agaactacct ggctcaacgg atacgggcgt
     3121 tggcggtcga aaatcagatc ccgctggtgg agaacccgcc tcttgcccag gccctttata
     3181 aggcggtcga cgtgggacag gagatccccg cgcacctcta ccgcgcggtg gcggaggttc
     3241 tggcgtacat tctccggctg atgaacggcc ggttgcaggg ttaggaggag tgaccgatgg
     3301 ctgagacaaa agcgatggcg tggttgggcg gctggaagct gccgcaggga gtcagcctga
     3361 cggcgctggg cgaactgggt gtgccactgg cggtgctggc gatcgtgctg gcgctcatca
     3421 ttccgctgcc gacgttcctg atggacctgc tcatcgtcac cgacatcatg atgtcagtgg
     3481 tcgtgatgat ggtctcgatg tacatccgcc gtccggtgga gttcagcgtc ttcccaacca
     3541 cgctgctgtt gctcacgctg ttccggctgg ccttgaacat ctcatcgtcc cgtctgattc
     3601 tgctcaacgg aaaccacgga accggcgccg ccggggacgt gatcgaagcg ttcggcagct
     3661 tcgtcgtcgg cggcaactac attattggcg cggtcatttt tctggtctta atcgccattc
     3721 aatacgtggt gatcaaccac ggcgcggtac gcatctcgga ggtcacggcg cggttcaccc
     3781 tcgatgccct gccgggaaag cagatgtcga tcgactccga cctgaacgcg ggcatcatcg
     3841 acgaagcgga ggcccgggac cggaggcgcc agcttgtcgg cgaggctgag ttttacggtg
     3901 cgatggacgg cgcctcgcgc ttcacccagc gcgacgccgt ggcgagcatc ctgattaccg
     3961 gcatcaacat cgtcgccggg tttttgatcg gcatcctgca gcacggaatg gattttcgcc
     4021 gggcgctcga aacgtacacg gtgcttacca tcggcgacgg cctggtcacg gtgatcccgg
     4081 cgctgatgat ctcgatctcg ggtggcctga tcgtgacccg cgccagctcc gaggcccggc
     4141 tgggcgcgga gtttcgaaag caggtgttcg ggaataccga acccctaatg cttgctagcg
     4201 ccgtgctcgt cgccctggcc gcctttcccg ggctgccgaa gattcccttc ctgttgatgg
     4261 gcgtgggact cggcgtttcg gcgtggcgaa ggaagaagcg gtcgatcgag tcggagaaaa
     4321 cggcggctgc cgccaagcct gccctggcgc gagagaatct cgaagcgctg ctgcgggtcg
     4381 agccaattgc ggtcgaggtc ggcctcgggc tcgtacaact cgtcgaggga gggcagaact
     4441 cgctattgtt gcggcggatc ggcgggattc ggcggcagct tgccgcggaa ttgggctaca
     4501 tcctgccgcc cgtccgggtc acggataacc tctcgctcgg cgcacgcgaa tatgtcgtac
     4561 tgctgaaggg cgagcggatc gccaaatacg aactcccgta cggccgagag ctggcgatcc
     4621 ccctcggcaa gcccgatcca agcctggagg gacaaccgac ccgtgaaccg gcctttggaa
     4681 tgaacgcgct ctggataccc tcggaacgag ctgagcgcgc ccgaagcctc ggttacacgg
     4741 tggttgacgc cgtcagcgtg gcgggaacgc atcttgccga gatcgtgcgg cgccacgctt
     4801 gcgagctgtt ctcccggcag gatgcgaaaa agctgctcga ccgggtagcg gccgagaacc
     4861 cgagggtcgt cgaggatctc gtgccgaaac tgttgccgct cgccaccgtg cagcgggtcc
     4921 tgcagaacct gctccgggag cgggtcgcca ttcgcgacgc ggtttcgatc ctcgaatcgc
     4981 tgagcgaagc ggccgccgtc agcaggaatc cggtgttgct gaccgaatat gtccggcaat
     5041 ccgtgcgccg ggcggtgatc aagccttacc tgaacgctac cggggagctg ccagcgtact
     5101 tcttcgaccc ttcgatcgag caggtcatcg agtcatccat cgagcacggc gagcatcaca
     5161 gccatctggc agcgcagccg caggtactgc gggagattct tacccgcatc cagaacagaa
     5221 tcggcacacc gggcgctccc gtggctgcgg tggcgaattc cggtgcccgg tactttctcc
     5281 gccaggttct ggaatcgtcg ctgccgggac tgttcgtgct gtcgcacaac gagattccgc
     5341 cgggggtgaa ggtggtctcc cagggggtga tcggatagcc gccggccggg ggcactaccg
     5401 gcttcgacgc gatccgacga tttatctagt gcggttcaga agaacccgtg ttcgcatgca
     5461 gttgccgagc cgcaaccgga cggagcggaa gctcccgcga actttttcag caccctgtca
     5521 gtacggtgga gcgctttgaa atcttcaagc cgacgaaatt gttctttgca gcaggccggg
     5581 gagtgacggt gtcagccggt gaggtcgtgt attctggttc gcacaccgaa gggacggttg
     5641 cggaacggga gcggctgatt ctcgaccatc tgccgcaagt gcgtctgatc gcccggcgaa
     5701 tccacgagcg cctgccagcg acggtgagcc tcgacgatct cgtctcgacc gggatacttg
     5761 ggctcatctc ggcgatcgac aactttgatc cgggacagaa cgtcaagctg aagacgtatg
     5821 ccgagtacaa gatccgtgga gcgattttgg acagcctgcg ggacctcgat tgggcgccgc
     5881 gcgataagcg aagaaaggcg aagcagatcg aggcggccat ctcagctgcc gagcagcgcc
     5941 tgcagcgggt ggcgaccgag gaggagctcg ccgcggaact gtctctcgaa gtcgaagaat
     6001 accgggagtg gctggtcgag attcaaggac tgtctctgaa cagctatgag ttcggtttcg
     6061 gaggcaagga cgagcgcaat ctgctcgaca tcgtgccggc cggtgatgag cattcgccct
     6121 cgcaggtttt cgagctcgcc gaactggaga ggctcctggc gggtgctatc gagcggatgc
     6181 cggcggtgga gcgtactgtg ctcagcctct actaccagga ggagctgaca ttgcgggaga
     6241 ttgccaaggt gatgaacctg catctgtcgc gcatctcgca gttgaagtca caggcgatcc
     6301 tgcggctgag gtcttttctt ggaaagcgct ggccaacccg gcgcgggagg tgatgctcat
     6361 gaatccctcg gatcttcttg aggcgctcgg gaaacccggc gcctccgatc cggcccggca
     6421 aggcgacgac gctccggcgt ccggtctgaa ctcgaagacg ctcgacctgc ttctcgacgt
     6481 cgaactgccg gttacggtgt ccttcggccg ggcgttgttg ccgctccagg acgttctcaa
     6541 gctgtccgcc ggctcgattg tcgaactcaa tcgcaccatc agcgagcccg tcgagctcat
     6601 cgtgaacaac tgcgtgatcg ctctcggaga agtcgtggtg gtcgaaggta actatggtgt
     6661 gcgaatccgg cagatcgcca gccgggaaaa tcggttatcg acaagcagcg attcgctcct
     6721 cccggcccag tttccccgag gttgaacgca gcttggccga cagcgtcgtc cgcttcaggc
     6781 gcagcaggtc ggctgcccgc ttcttgttgc cgccggattt tcgcattgcc tgatcgagga
     6841 tcgagcgctc gaagcaccca actacctcgt cgaaatccag tccgtggtcg ggaaccgtaa
     6901 tcgatggcgc cgattcgcac cgggacggct gctcacgcac cggcgacggg agcgggaaat
     6961 cgctcgggta cagcaccggc cgctggccgc tcagagccac cgccatctcc accgcgttct
     7021 ccaactggcg gacgttacca ggccaggagt atgacgacag gcgctcgagc gtttcgccgc
     7081 cgacctgctt cgccgggatg ttctcgatgc ggcagatctt ttcgatgaag tgcaacacca
     7141 gcaacggcac gtccaccagc cgttcccgga gcggtggcat ctcgatgggc accacgttga
     7201 tgcggtaata gaggtcctcg cggaactgtc ctcgccttac cttctccggc aggtcgctgt
     7261 tcgaggcggc ggcgacgcgg acatccaccc ggacggtctc cgagctgccg aggcgctgga
     7321 attcccgctc ctgcaagacg cgcagcagtt tcgcctgaag ctcgagcggc atgtctccga
     7381 tctcgtcgag gaacagcgtg ctcccgtcgg cttgttcgaa acggccgatc cggctctgga
     7441 cggctccagt gaaggccccc ttgacgtggc cgaagagttc ggcttctagc agatgctcgg
     7501 gcaacgcgct gcaattgacc gtgaccatcg gcaggtgacc acggggactg gccaggtgaa
     7561 gtgcccgggc cgccatctcc ttaccggttc cggtctcacc cgtaatcagc accgtcgaac
     7621 ggcgcgggcc gacgagccgg atggtttcga tgacgctccc aacggcccgg ctcgcgccga
     7681 ccagccagcg gcgccacggt tcccggccga gtgccgcgct gagcgacgcc aagtcggtgc
     7741 tgcggcgctc ctcgacggct gcctcgatcc gccgggccac atcggcggcg caaagcggct
     7801 cgccgaagta gcacagggcg cccagccgcg cgaggcgcac cgcccggtca aaagcgccgc
     7861 tgtcctcccg gatcagcacc ggcgccgacg cgctggccct ctggatctct tcgagtgttt
     7921 cctcggctgt ccatcccgga atcgggagtg tgaccaggac cgcgtccgct ccggaggacc
     7981 ggaggtggga tagcgcaggg gcgatatccg ggaagacgtc aacgtcgaca ccttctagcg
     8041 ccgccagcac ggcggcatct tctggagccg gcgttccggc gcatacccat accagtctgg
     8101 tctttgagga catggggctg tgacaaaaat cggtcagtgg caacggtttc tttaccgcgt
     8161 gacggccgga ttgggttgga aaacatgaga ctcagtaaga atcctctaaa acctgaggcc
     8221 cctcctgccg atatgtgggt tgaggagagg aggagcagcg tttacggagt tgcagtaatg
     8281 caatgagagt cgatgatcga aatctcaccc ccggcgccgc ttcacaaacc ggcaagacgg
     8341 cggcgacgca ggatgtagat cgggtcccgg actcgcgctc cactggaaca cgggaaaccg
     8401 gcgaaggtga cgaagtcgag ttgtccggcc tggtggcgcg tctatcccgc gccatcagcg
     8461 ccgcttccac ggaccggacg gaccgaatcg aggaactttc gaatcgcttc gagcagggcc
     8521 gctaccaggt cgattccctc gccgtcggcc gcgccatcct ttcggagttg cgggccgccg
     8581 ggcacgagcg tccggacgga gccccggccg ctctctgacg gcccgcaggc ccggagcttg
     8641 ccacgatgaa atccacggac accatcgata gccttgggcg ggtacgcagc ggcgtgcgct
     8701 gggcgctcgc tcaaacagcc acgccatcct cggagatcct ggaccggtgt ctgccggttc
     8761 tcaaggcggc tgaagccgat cttcgggatt ctgccccgcc gctcgcggga gccgcctctt
     8821 ccaagaacaa gagggaaatg cttcggcagc tgcgggagct gaaaaaggat ctgggctccc
     8881 tcgccacaat gctggatcac gcggccggtt tctacgacgg ctgcaagcgc ctgcacgaac
     8941 cccaggacga cggctatgac gcccgcgggg cgcctcagcc gtcctgcccg gcttcccggg
     9001 tttcggtcga ggggtagtag gaacggggag accccgtccg gcggaacaac acgatgccga
     9061 atctgctgaa cagcctgctg tcatccgcta acgcgctgcg ggtattcgag cgcgttcttg
     9121 ttgtttccca gaataatgtc gctaacgcat ccacccccgg ctacgccgcc cagcgtctga
     9181 gccttcaggc tgtcgatttc cagcccgaac tggggctgat gggtggagtg cgggccgggc
     9241 atgtggaaag cgcacgctcc ctctatgcag agcaggcggt ccgccggcaa tgcgagtctc
     9301 tcgggttctt ctcacaggcg gcgcaaagcc tggcgagcat cgagtctgca ttcgacatta
     9361 gcggacagcg cgggatctcc ggtgcgctca accgtttgtt cgagagtttc tccgcctgga
     9421 gcctgacccc gaacagtgcg aatgcgcgcg cttcggtaat cgagagcgcc gggcagctca
     9481 gcgacgcctt tcatgagacc gcatccatgc tggccggtgc tactatcgac gccgaccggc
     9541 agattcgcga aaccatcgcg cagatcaacc ggatcggcgc ccggctgcag gaatacaacc
     9601 tggagggccg gcgtggcgcc atcgaggaag cggggctcga cacgagaatt caagccgctc
     9661 tcgaagaact gtccgagctg gtgaacttcg acgccatttt tcaggccgat gggacggtca
     9721 ccgtccttct cggcggggag actccgctcg tcgtcggcgc taaccggtat gaggttagcg
     9781 cctcattctt tacgccgccc aatccccctc ccgtctacgc cgccgcgacg ccatcggctc
     9841 atgtcctcga tgccaacggg cgcgacatca cctaccagat cgaccagggc aagctcggcg
     9901 gcctgctcaa cgttcgcaac gaggttctgc cgtcgctccg tggcgacggc cagcagccgg
     9961 gtgagctgaa ccagcttgcg atgtcaatcg cggaccgcgt gaatgcgatc ctcacttcgg
    10021 gccgggtcag cgacgggccg ccgccggaga acggcgtgcc gctgttcact tacgacgccg
    10081 gaaaccagac gctggccgca cagagcctcg cggtccgccc cggcatcacc gcagaacagc
    10141 tagcggcgat cgaccccgga cctccttacg taagcaacgg aatcgcgctc cgcctcgccg
    10201 cactcgccag cccgcgcgac ccgtccgaca agatcgacgg cttcagctac acagaattct
    10261 atgggggcat cgccggccgg gttggccagg ctctgagcga agcccgggag ggccaggaat
    10321 tcaaagcgca aatggtgtcg caggcgcggt atctgcgaaa tgagatctcc ggtgtcagcc
    10381 tcaacgagga ggcgatgcgg atcacggaat tccagcgggc ttaccaggcg aacgctcgaa
    10441 tggtgacgct gcttgacgaa ttgacggaaa cggccgtcaa tctgctccgg tagcgagaaa
    10501 ggcgaaatca tacctatgat ccggaacctc gatccctctg ccgagcggtt cctgctggac
    10561 ctgcggcgca tccagaccgt caccgggcac gcacagcgcg agatcagctc cggcctgcgt
    10621 gtcgggagcc cctccgacgc accggatcag atcggagata tcctccagct tcgcgccggt
    10681 attttgcgtg cggcacagat ccgcatgaac ctgggccgtc tgcaaaccga aaccgccgcg
    10741 gccgaagacg ccctcaatct gtccgtgaag gtcatggacc gcgcccgcgt gctgggttcg
    10801 cgaggggccg gctccaccga gacggccgaa gcccggcaga tcctggcagc cgaggtcgag
    10861 gcgctgctcg agcaactcgt gtcggcgagc cggactacag tggaaaatcg ctatgtgttc
    10921 agcggcgacc gtgaccgcga accggcgtac gagcttgacc tcgctgtccc cggcggcgtg
    10981 gatcggaaat tcaccacaca ggcgacccga cgggtagagc acccgaacgg gtcctcgttc
    11041 gaggtctccc gaaccgccca ggagatcttt gaccttcgct tgctggacga tacacccgca
    11101 ccggagaacg ccttcacggc gctcaacaac cttcgtgtcg cccttcagaa caatgaccag
    11161 gcggcgatcg acaccgcgtt gcccgcgatg cgcctcgcgg gtgactatct gaaccacatg
    11221 ctgagtttct atggcagcgt gcagcacaaa gtcgatgagg cgttcgactc cgccaaacgg
    11281 cttgagacac agttgacaat cgagctcggc gagaagcgcg acgccgacgt agcctcggcc
    11341 attgtggagt tgcagcaaag cgtggcccag gaggaggctg cgctcggggc gcgtgcgcag
    11401 ttgccgagaa ccagcctgtt cgactacctc ggctgagttg tcgtcgtcga gaacagcggt
    11461 tccgccagtc ctctcaacgc ttcgacgccg tctctcgggc ggcgaggaaa cgggagatga
    11521 aattcgtgtc gaagttgccg gcctggaagt ccggatcggc catgatctgc tggtgaagcg
    11581 aaatcgacgt ggaaatcccc tccaccacga acatctccag ggcgcggtgc attcgggcga
    11641 tcgcttccgc ccggtcgcgg ccgtgtgtga tcagcttggc gaccagcgaa tcgtaatacg
    11701 gaggtattac acagtcggag tacgcggcgg tgtcgacgcg gacgcccatc cctcccggaa
    11761 tgttcagcgc cgtgatgcgc cccggcgaag gtgcgaacgt ctcagggttt tcggcgttga
    11821 tccggcactc gatcgcatgc ccccgaatcc tcaccggctc tgtcacaatc tcgcttagcg
    11881 gcattccgga agcggccaga atctggccct tcaccaggtc gacgccggta atgaactcgg
    11941 tgaccggatg ctccacctga attcgggcgt tcacctcgat gaagtacagt tcgccgttct
    12001 cgtccatcag gaactcgacc gtgccagcgt tcgtgtagcc cacggcgtgc agcgcctcgc
    12061 gcaggctcgc cgcgacccgc gccctgagtt ccgctgtcac tttcggtgac ggcgactcct
    12121 ccaccagttt ctggtgccgc cgctggatcg agcattcccg ctccggaaac acctcgacgt
    12181 tcccatactg gtcggccagt acctgaaact cgatgtgccg cggcgcctcg acgtacttct
    12241 ccaggtagac gtcgggagac gagaacgccg ccccggcttc ctgctgtgcc tgtgcgaaca
    12301 gtccggcgag ttcagccggt tcgcgcacga tgcgcatgcc gcgtccgccg ccgccggccg
    12361 aggccttcag aatcaccggg taacctatct ggcccgccac cttttccgcc tcttccggcg
    12421 acttcagtac gctgtccgag ccgggcagca cgggcactcc cgcggccgcc atcgccgccc
    12481 gcgccttgtc cttgactccc atcagccggg tcacctccgg ccgcgggccg ataaagcgta
    12541 tgttggaaag ctcgcacacc tcggcgaagt cggcgttttc gctcagaaaa ccgtagcccg
    12601 ggtgaatcgc gtcgacgttc gtgatctccg cagcgctgat gaccgacggg atgtccaggt
    12661 agctcagtgc gctcttcgcc ggcccgatac agaccgcctc gtccgcgaaa cggacgtgca
    12721 agctgttgcg gtcggcctcc gaatacactg caaccgtgcg gacgccgagt tccttgcagg
    12781 cgcaaatcac ccggagcgcg atctctcccc tgttggcgat cagtactttc ttgaacatag
    12841 aaacgagtcc ggctgcgcgg gcttacagtg gacggacagc aaacagcgcc tcgccgtatt
    12901 ccaccggctg cgcattggcg accagtctct tgacgaccac gctggcgatc tccgcctcga
    12961 tctcgttcat cagcttcatc gattcgatga tgcagagcac ctggcccgga tgcacaatgt
    13021 cgccggtctg cacgaacgga ggtgcgcccg gcgacggcgc ttcataataa gtgccgacga
    13081 tcggtgattt cacgacgatc aaggaattgt cgtcggtttc ggcttccgct tcatcggtcg
    13141 cgggtgcggg ggccgttgct ggcgtcgcat ccggatacgg gttcgcggcc ggaatcggcg
    13201 cgggcatggc cgccgtgatg gccggcacca cgatatgttg ctggctggca accggcgcat
    13261 tccggaggat gcgcacgcgg ttgtcgcccc gctgcacctc cagttccgcc acacccgtct
    13321 cggtgactac tttgatgagt tctcggattt cgtcgatggt catggattcg aaactgctgg
    13381 attcgggagt gttcagagtt cgatcagctc cttggaggcc ggggtgagga tctcacagcc
    13441 gccgcggttt acgacgacca tgtcctcaat acgaattccg ccgaatccct gcaagtatac
    13501 gccaggctcg acagtgattg ccatacccgc cttgagctgt agctcgactt ttctggcaac
    13561 tcgtggagat tcatggattt ccaggccgag cccgtgcccc gtggagtgga cgaaggccct
    13621 ttccaggccg gccgtgcgca gtaccctgcg ggccgctcgg tccacgctct ttgccgccgt
    13681 cccctcccgc acagcctcta gcgccgcctg ctgcgcctcg agcaccgcgt ggtggaaccg
    13741 cttcgctttc ggtcgtgccg cgcccaggaa aatggtccgc gtcatgtcgc tcgcatagcc
    13801 ttcgaggaac gcgcccatgt cgatcagcag aatctcctgg tttcccagta ccttcgccgt
    13861 cggactcgcg tgcggcagcg ccgaccgctc cccggacgcg acaatcgtct cgaatgccgg
    13921 cgactccgcg cccagccgcc gcatccggta gtcgagttcg gccgccagat ccaattcccg
    13981 tatgcccggg cggacgtgct gcagcgtctg ctcgaaggca ttacaagcca gcgcggcgga
    14041 gcgccgcatt gtttcgatct ccgcctcgct cttcaccatt cggtactctt ccactaggcc
    14101 gtcgacaggc atcgctgcga cctttgacgg caggtgtgcc ttcagagtcc ggtaggcgtc
    14161 aaacgtcagg tgagccgctt caaagccaag ccttccccgc ggcagcgact tcgccacgcg
    14221 tgggatcagc gatccctgta caaccttcac cgtgcattcc gtctcctcgg tcgcctgaat
    14281 cctgtaccgc ccgtcggtgt acagcacggc cccatccgga gacagcagga gcagcgcatt
    14341 gctcccggtg aaccctgtca gataacggac gttgaccaga ttcgaaatca aaaacaagtc
    14401 tagccgccgg cgcgcaaggt cggcggtgac aagtcttcgt cgagagatga attcagtgga
    14461 gatcactcga tggaaccgcg accgtcaggg agcggtaagc actcaattac cagtctacca
    14521 gccgctagag ttacagtgat acgttatctc cggaggcaaa atcgatgaga aggcgagatg
    14581 cattgaaggc gggtctggcc gcggctccct tgttcgtgcc gtcgcacgtg ctaggcggcg
    14641 ccggacgcgc tggtgcaaac gaccggattc agatcggctt tatcggcctc ggcggccgcg
    14701 cccgctggct aatcgagcac gaagagttcc ccggtgccga catcgtagcg gccaccgact
    14761 gttttccccc gcagtacgag acggccgcga aaatcaagcc cagcggggaa aagtggcgca
    14821 aatactcgga ctaccgccgc atgctggaca aggagaagct cgacgcagtc ttcgtcgaaa
    14881 ccactacgca cgcacgggtg ctcatcatga tccacgcgtt gcaggccggg ttcaatatct
    14941 atggagagaa gccgctcacg ctcaccatcg ccgaaggccg cacgctggtg aacgcagtga
    15001 agcgttacaa ccgggtcctc cagaccggca cgcagcagcg ctccatcccg gtcaacgcct
    15061 gggccagcaa gatgatccgc gagggtatcc tcggtaaggt ccaaaccgtc atcacctgca
    15121 acttcgagcc gcccgtcggc tggtacccga aaccggcaca gaagatccct gagggcctcg
    15181 attgggacca gtggtgcaac cagaccgagc tgcgcccgta tcattctcaa ctccggcgga
    15241 attgggcctt gtggtgggac tacgacggcg ccggccagtc atggggcgtc accggatggg
    15301 gcacacactc gctcgatcag gtgcagtgcg ccctcgggac tgacgacacg ggcccggtcg
    15361 agctatggcc tgagtccgag tcgttcaccg ctgctgtcac tgctcgttac gccaacggca
    15421 cactgctcaa gatggaaggc cccaagcgtg atcacgcgga cctcggcgcc atcttcatcg
    15481 gcgagaaagg gaagatcgag attaaacgcg gcacgtgtgt cgccgacccg cccgatctgc
    15541 tcaaggacgc gccgcccgac actccggaag gccccggcga gaaccgctac cacatcggca
    15601 acttcctcga ttgcatccgc accgggaaac tccccaacgc gcacgccgag gtcggacacc
    15661 gctccaccac actctgccac ctggtgaaca tgtgccggga ccttggccgt aagctccagt
    15721 gggatccgaa agccgagcga ttcatcggcg atgaacaggc caacagcctg ctctcccgcc
    15781 cgcgccgcaa aggctacgag ctgccgacaa tctgagcgcc gtttacttca ggaccggcac
    15841 ccacaggtga gcggttgcct tctcgccgcg ttcgttcgcg cgctccacgc gcacgatgta
    15901 gtcgcccggc ttcttgaaaa cgtgctgtgt tcgcgcgtac ccttccttgg ccttttcctc
    15961 cgcgcatccg tcggaggtga cggtcactgg ggcgctgccg tcgccgaagt cccacgtctc
    16021 gtgtccgaat gtggtccggc acgtgcgcac cagaaatgtc accgggtctc ccgcgcggac
    16081 attgagcgcc ggccaggagg aggcatgaat cgtcgggggc acacgctcct ccgtgcccgg
    16141 tttcgtgctc tgccgctcga cggtctgcac tactgcgaag tcataggcga taagtccgcg
    16201 gctgtctgtc acttggagga tctcgctgta cgttcccggt ttccggtact tccgcgtgat
    16261 tgttgggcca cttgccgacg tcccgtcgct gaatcgccat tggtaccgaa cgatttgacc
    16321 cgagtccacc cacgaccggc tgccgtccag cgtcaccggc tcccccaccc aggcgaagga
    16381 gtggggacgg gccaccgcga cgatcttcgg gtgatactga ttccagtaag cctcccggag
    16441 gaatgcgtaa ccttccacaa tgcccgcctc cccggaaggt tgcgggccgc ggatctcgta
    16501 atgcaggtgg gaccaacaac ctgcactgcc ttctttgccg atgagcccga ttcgctgccc
    16561 gatccggact ctcttcccga gttccaccgc ggtatcgatc gaggcgaggt ggaagtacca
    16621 gtgaaaccag ccgcgatcat cgagaacaat cacgccgtcg tagctcacct ctgtgtacgg
    16681 actcttcacg tactcgggaa gcgcagtctt gccccggacc accacgcgcc cgtcggtcgc
    16741 cgatacgact tcggtcaggc cttccgcacc gccgatgtcc aacccgtagt ggtaatagat
    16801 ttttcgcccg ctcggctcct ccgagccgtc tacaaacgac ggttcgttcg ccatctgcgt
    16861 atcggttgcg aaccatcgct gcttcaccgg atacgtgtaa gttcctggag ccagcagagg
    16921 agacccggcg ggccacaatc gcagacgcgc gtccttgcgt agtccccagg ggttggttcg
    16981 gctattccgg aggaggtctt tcgtcacggc gcagtcgatc tggacgccgg cgacggttac
    17041 cggcaaccgg tatgccccgc acacgagtcg gatttcctcc tgcttgatct tgacgcggac
    17101 gctggcgacg cgtacagcgt ctctgacgtt atcggcgacc gccgagtgct ccagcagttt
    17161 cacctgggac acttcatccg tctcaccgac atccaggtcc agtacgcgca tgggctgcgc
    17221 catcgcaatc tgcacagcca ggaaaaccgg gacagtacac tcaattccga atttcacgct
    17281 gccctcgctt cagggtttga gcagaagctt gcccgaagtc ccacgccccg ccaggtcgat
    17341 ctgcgcctgg gcggcttcgg caagcgggta ggtctggtga acgcggacct tcagcttgcc
    17401 ggatccgacc cagcccagca cgtcgttcgc ccgccacaat agctccttcc gttcgagcgt
    17461 gtaatgcatc agtgtcggac gagtcaggaa cagcgacccc ttttggctca gtgcaagcgg
    17521 ctgcgtcgcc ggcaccgcgc cgctggcgtt gccgaacgtc accatcatgc cgcgcggccg
    17581 caggcagtcg aggctcttct cgaacgtcga cgcgccgacg gaatcgtaca ccacgtccac
    17641 accacgcccg ccggtcagcc gcctcgcctc cgcctcgaag tcctgcttcg tgtacaggat
    17701 cacctcgtcg gcgcccgcgg cgagcgcgag ttgcgccttg gcctcggtgg aggttgtccc
    17761 gatcaccctt gcgccccgca ttttcgccat ctgcacgatc agcaggccag tgcccccggc
    17821 tgcggcatgg accagtgccg tgtcgccctt cttgagtgga aaggtggagt gcgtcaggta
    17881 atgcgcggtc atgccttgaa gcatcgccgc tgcggcggat tcgaagggga cggtgtccgg
    17941 cagctttacc agcatcgccg ccggcacgga ctggtactcg gcgtaagacc cgcgggccat
    18001 cgcatacgcg acgcggtctc cgggagcgaa ctccgtgacc tctgccccta ccgcctccac
    18061 cgtacctgcc gcctcctggc cgatggagat cggaagatcc gccttgtaca gcccgctgcg
    18121 gaaataaatg tcgatgaagt tcacgccgat ggcggcgatc ttcaccagcg cctctttcgg
    18181 ccccggctgc gggacgggaa tatccgccag cgtcagcgcc tcaggccccc caggttcgta
    18241 caccagaatc gccttcatat cggcaagtag agtagcacga aaaccggaaa accgggacag
    18301 tacactcaat aacgcattta tttcagtgac aggctacgca atcgctaaac tcaacgtccc
    18361 gttgccggtt cccgaaccag ccgcccaccg cgccataatc gcaccatgag attcgtagcg
    18421 cttctgctgt tgatcctcgc cgacgcctcc ggccaagccc cggactggct tcgcctcggt
    18481 ctgctcaccg cgatgcccgt gaagtgggac gtcgaagcga actggaacac cttccagcgg
    18541 acccttgagg cccatgcctc cgacgggatc gatctcgcag tcacgccgga gtgcttcctg
    18601 gacggctacg ccgtcgccga caaggaatgg actcgcgaac gcttcgaggc catcgcccaa
    18661 cacccggaaa agagccccta tctcgcccgg cttcgggacc ttgcccggaa acaccgcgtc
    18721 ttcatcctct tcggatatac cgagaaccgc ggcggcaggt tcttcaacac ggcgctgctg
    18781 atggaccgcg acggtcgcac ggtggggcgc tacgacaaaa cgcacctcca ggaccacgac
    18841 ctccgcttca gtcccggcga ggacctgccc gtgttcgaga cgccgtgggg aaagctcggt
    18901 gtgctgatct gcgccgaccg ccggtggccc gagacctccc gcgtcctccg cctccggggc
    18961 gccctcctgg tcctcatccc ctcgtacggg atgtggcacg aagccaacga gtggtggatg
    19021 cggacccgct cgtacgagaa cgagaatttc ctcgccttcg cccatccaaa cgtcgccttc
    19081 gtcgctaacc cgcgtggcga gattctggcg aagttgcaga cgaacctccc cgcaatgctc
    19141 gtctgcgatc tgaacctcac cgaggtcacc ggggacaagc acctgcgcga ccgtcggccc
    19201 gagctttacc gtgaccttgc cgcaccgaag tagaagcaga ttcagtacac tcggtccttt
    19261 acagcggaaa accatgaggg aatcatgacg gtcggcaaag cagcatcggc tttcttcctt
    19321 tttgtccttg cgggagtgtg gacggtcccg ctctcagccg ctggcgagca ggcgtgggct
    19381 gtaccgactt tcgaatcgct ggggctttac tacaaccgcc ccattgcgaa ggacccgtgc
    19441 cgggcgtggt atcgggtcgc cggtacccag gagtggcgcg aagggcaagc tctcgtctat
    19501 gacgagcggg aaaagcaata ccgaggctcg ctcgtcggcc tcactgcgaa cacaccatac
    19561 gaaatccggc tcgaagcaga cgggcagaag atcgagttcg agacccgtac ccgtagcgaa
    19621 acatttcccg tgggaaaaac cacctatctg ccgggggacg gcaccgatca gacgctgtac
    19681 atccgcgacg ggggtacggc cgacgcctgg cacgtggtca caccgcggcc agggacgaaa
    19741 ttcgtgagcg atgtctttaa tctctccgac tacaacgtgg tcgtggaagc ggattatgtc
    19801 atccttcgcg gtctcgaact gaagaacgcg ggcatgcacg gcgtcctgat ccgggcgggc
    19861 gtccaacacg tggttgtgga agactgccac atcacaggat ggggacgcct cggcggcgcg
    19921 cgagtctggg gcgtcctctt cggatcggac agtgcggtct acgcggaagc cgacgccggt
    19981 cacttggtga ttcaacgcaa cctgctcgag tacccgcgcg gtggggccaa cgactgggaa
    20041 tccgggcacc cgtccgggcc gcaggcgatc acgttgactg attcgcgcgg cggcaacgtc
    20101 attcggtaca acaccatccg gtcgaccgac gatcacggct tcaacgatgg aatcggcggc
    20161 agctccaact tcagcttcaa aggcagtccg aaccgtgact cggacattta cggcaacgtc
    20221 atctccaact gctgggacga cgccatcgaa agcgaaggcg caaacatgaa cgtgcggatc
    20281 tggagcaatt acatccacca cacgtttgtg cacatcgcga cggcggccac gtcgatgggc
    20341 cctctctaca tcttccgcaa cgtgttcggg gagagcagga tctcgcacca ggactcctcc
    20401 ggaggcatga tgatcaagac cgggatgaac tatctgaaca tcgcgggaga gcgcgtcagc
    20461 actggcctgg gttaccggtt catttatcac aacaccgcac tccagccgaa tggcggtctt
    20521 gacgtgttca gcagccacga gctgcacaac gcggtcagcc ggaataacat tttctatgcg
    20581 cgcggtcgtg cgtatcccgc ggaccggggc gagccccgca acgactttca aaatgacctg
    20641 acgggcagct acctcggagg agggatcgtc cgctcgatgt tcttgcccag cgaagggctg
    20701 gagtggtatc cggcgccaac agccagccgc atccaatggg gcagggtaga gtacactcgt
    20761 aacggccgga atttcgcgat taccgatcct ttggtacagg cgaaaaaccc ggcgctcgat
    20821 ggcggagttc gcttaccggg cttcaacgac gagttccagg gagccgctcc ggacattggc
    20881 gcctttgagg cgggactgcc tccgttgcac ttcggccgcg agatggcgcc cgggttcact
    20941 cgcccgccct gggagctgta ttaaccaaaa cccgggacag cactcatttc cgggtttcca
    21001 ggcagacagt gtccgtgtag acggggatgc gccggctact tgggcgcgag ggtgaaatcc
    21061 acgtgtacct ggatgccgtc ccattggtag cccgcggccg tcactcccgt cactgtagcg
    21121 gtgtacggac ctggattcgt gcctccgatc ccacgcttat tgggcgcctt cgtctgccag
    21181 gtcccctcgg cgacgccgct gctgttgctc gggccgctcg tcagcgtcgc c
//
LOCUS       DATIFQ010000018        17849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1921127, whole genome shotgun sequence.
ACCESSION   DATIFQ010000018 DATIFQ010000000
VERSION     DATIFQ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17849
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921127"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VLE22_00680"
     CDS             <1..358
                     /locus_tag="VLE22_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSB12937.1"
                     /translation="VAFTSSVLRFAVALGSMILVNAMMYRLGPNRPQRWRNVWPGAIV
                     ATVLWWVATQGFAWYVRNIADYNLLYGSIGAVIALLVWMYVLAAIAFIGCEFNAEYER
                     MVAAVRPAAPANTVSR"
     gene            complement(339..1418)
                     /locus_tag="VLE22_00685"
     CDS             complement(339..1418)
                     /locus_tag="VLE22_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB12938.1"
                     /translation="MTHRERVIAALSHEPPDRCPMQISFTPEFADRLRADLEIKGRKT
                     HNPHGGGNTYELEHAIGEDMLLTSVGWANCYYQDGERYVDEWGITWKQMPYETPYGAG
                     YYTETVGRPLADASALSRYRPPDPSRPELYAEAEWLLRESQSEYWIVGVTVTTVFETA
                     WALRGYEQLMIDFLEDPDLAESILEIPYRYHLTAAKTLVRMGVDMIWIGDDVGAQNAM
                     LISPAHWRCFLKPRMASFIATLKGINPRLKIACHSDGCIYPIIPELIEVGVDVLNPVQ
                     PASMDPARLKREFGSRLCFWGTVDEQQTLPFGSPDDVRAEVSERLRTVGDGGGLILGP
                     THHVQLDTPMENFWAMVKAINETPY"
     gene            complement(1435..3543)
                     /locus_tag="VLE22_00690"
     CDS             complement(1435..3543)
                     /locus_tag="VLE22_00690"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSB12939.1"
                     /translation="MNLSVAILLLLSMPLAAGPLRIVPDEFLFGASVYPELQTREEWN
                     RMLDVFQKARFKAVRVSESSWGNLETAPGRYNFGWLREFLNDLDRRGMKAVLGTSSYI
                     APQWLSAKNPDMLVELNPGEKLHPMGRKAACLNHPLYRQALRRYLLALGGEFKSHPAV
                     IGWQLDNEIEFLVGRPCYNSACERAWKDWLKKTYRTPEEFNRRLRLVSWGMEVDSLDD
                     VPQPRTVFEMPQAGRSGLPALSLAHLHFQRDSILEFFAEQTKALREAGVKHWITTDWN
                     TMWQALADDPRARESLDIAGLNFYQPSSDEPDFWTTLGWHLDMHRSAHGLGSFLVTET
                     RVGVAGDTVMWDPFPTKEQFRMWMLQPVAYGAFGLMYWTGNRWHGGHWPHWGGILDWT
                     GAPEPDFHWLVELGEFFDKWSGPLLRHPVKTAAAVITDFDQRAALITYPHVPSSRQVL
                     PQAFDALHRLGIGVDSVSVEDASKPDRLGKYTLVVIPTATALDHTGLMRALEAYVRNG
                     GNVVVSPFTAYQSSDGIFRNDGFGANLTAITGAVARTARRMGTSRDTGRMDQQVDWLG
                     GTSPVGIDGYCEYLKVDPDVEVIGRFRSTEPVLDSQPAATRKRLGKGAAVKLAFWPND
                     DSVVRLFQQLAPPTEGLLAGPCPAGFQAVPRSDGSLFVINTTPRPGAVQFVRGVSERI
                     SGRKMSGTVELKPYEVLWVE"
     gene            3619..5622
                     /gene="uvrB"
                     /locus_tag="VLE22_00695"
     CDS             3619..5622
                     /gene="uvrB"
                     /locus_tag="VLE22_00695"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HSB12940.1"
                     /translation="MAFRLAVDYRPRGDQEAAIEQLLRGVRDGEQHQVLLGVTGSGKT
                     FTMAKVIEQAGRPSLVLAHNKTLAAQLYHEFRTFFPGNAVEYFVSYYDYYQPEAYIPS
                     GDVYIEKEATINDELDKLRMSATRSLFERRDCIIVASVSCIYGLGSPEAYYGMLLMLE
                     KGRQIPRQQILQKLVEIQYERNDADFRRGTFRVRGDVIEVYPTYDDYAYRIELWGDEV
                     ESLSQIDPLLGQVRQTYLRLPIYPKTHYVMDETTREKALASIEQELRWWHPELEKQGR
                     LVEAQRLWQRTMFDLEMIREIGYCHGIENYSRHFSGRLPGEAPPTLLDYLPHDSLLFI
                     DESHQTVPQLRGMYHGDRSRKETLVQFGFRLPSALDNRPLTFEEFEHRVNQVVYVSAT
                     PGPYELTKSAGVVAEQVIRPTGLVDPEVEVRPIRGQVDDLLNEIRKRSESSERVLVTT
                     LTKRMAEDLSEYYSEVGVKCRYLHSEVSTLDRVKILRDLRRGEFDALIGVNLLREGLD
                     LPEVSLVAILDADKEGFLRSAGSLIQTVGRAARNINGKAILYADVMTDSMRKAIDETD
                     RRRRIQTEYNEINSITPQSIIKPIDMSLVAVAEGDYVTVPLEAEDEEVEAMAPEQRQK
                     FVEELEERMREAARKFEFERAAQIRDRIKAVKSIDVLEPSGTE"
     gene            complement(5638..6048)
                     /locus_tag="VLE22_00700"
     CDS             complement(5638..6048)
                     /locus_tag="VLE22_00700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB12941.1"
                     /translation="MITYRTGNNFDLDQVIELYRASTLGERRPVDERERMGEMLRNAN
                     LVVTAWDGDLLVGISRSLSDFAYVTYLSDLAVRVSHQNQGIGKELIRITQRESGPGVY
                     IVLLAAPKAVGYYPKIGFAQHPSAWLLRAGEQVV"
     gene            complement(6075..7754)
                     /locus_tag="VLE22_00705"
     CDS             complement(6075..7754)
                     /locus_tag="VLE22_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB12942.1"
                     /translation="MHTPSLLLLVLSAALACAQQPPPAAESAAYETQKKEKLCRITGR
                     TLHSQTGEPVRKVKLILHQMRMESKSYSAVSDAEGKFSIDDIEPGTYQLTAERAGFLR
                     QSYGSRRYGFSGTALTLMTGQELKDLEFKLMPQGVITGKVLDEEGEPVRGAFINVTRQ
                     VQSGGGRGAGMGGEQANDVGDYRVADLAPGRYIVQANPTPDFWGRQEGVEKSDEVREA
                     HVPTFYPGVTDAASASAVEVGPGQEVSGINITLRKSRVYRVSGSVSGATAGQLKMMNL
                     SLSPRDRKAMVMFFGGGGARANADGTFTIGKVQPGSYNLSLMLRGDGRPQVVGRAPVD
                     VGEADVEGVVVRVSQGLTLNGFVRVEGKEKIEIHGVRLMLRPTEGIGFGMQNAMVKED
                     GTFRMDGVMPDRYSIGTYNLPEGTYLKSVRFGNQDAPDKSVDLSQAQGSTSIELVLSP
                     NAGTVEGLVTDDEKPAQGISVLLMPDPPIADDPSQTKWANTDQNGRFSIKGVAPGDYK
                     LYASAESLTDVLQDPDALRPFEEKAVKVTVSEKGREQKELTLLKPEDARPK"
     gene            complement(7794..9476)
                     /locus_tag="VLE22_00710"
     CDS             complement(7794..9476)
                     /locus_tag="VLE22_00710"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB12943.1"
                     /translation="MRIAFLLFLVLPAALGCVQQIPPAAESAAPETQKKEKLCRITGR
                     TVHALTGEPVPRVRLILRRVVMDSRNLGAVSDAEGRFVIEKVEPGMYYLLADRSGFLR
                     RAYSSRGSGYSPTPLNLTANQEVDDLRFQLMPQGVITGKVLDEEGEPVQEASINVFRQ
                     VYAGGRGRTPGASGESTNDVGEYRAANLAPGRYIVQAAAEYDFRSEETTKPKKDEVRE
                     SYVPTFYPGVTDPASAATVEVGPGQEVSGINITLRKSRVYIISGKVIGATASELKMMN
                     VSVRSREHRMRPMVSSYVSPRVNPDSSFTLANVQPGSYNIVLMLGEGGRPQVVGRTTV
                     DVAEADVEGVILQVGQGVVLSGLVRLEGKEKAEVHGLRLTLRPVEDGEPFPYTAIVKE
                     DGTFRIEGVMPDRYSVAVYDLPEGAYLKSVRFGNHEAPDKSVDLSQTQGSGSIELILS
                     PYAGTVEGLVSDDGKPAPGSSVLLVPDPPKPEEPYKWKWVNCDQNGWFEIKGVVPGEY
                     KLYASKESLSGADEDPEALKPFEEKAVKVTVAERGRERVEIELAKSGDAQTP"
     gene            complement(9538..10545)
                     /locus_tag="VLE22_00715"
     CDS             complement(9538..10545)
                     /locus_tag="VLE22_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HSB12944.1"
                     /translation="MKHLSLLAITTLACAGSVFAQGKKILVTGMPSEAVAEFQAVAPG
                     ATVKAVDSAQLKNEIADADALLGTINADLVRAGKKLKWVQMYFAGMENVLSPELVNSD
                     IVLTNGKIIQGPEIADHAMALLLALTRGLYRTIPARTSEQWKRGTEQGMVELNGKTAV
                     VIGVGGIGTQIAVRANGFGMRVIGVDPKDKPYMPFVQKMVTPERLDEVLPEADVIFIS
                     APHTPKSRGMVGSRQFDLMKKGSYFVAVSRGKIFDNNALVRALDSKRLAGAGLDVTDP
                     EPLPPGHPLWKFENVVITPHTAGTSDRIQERRVALVKENVRRFVAGEPLLNVVDKQAG
                     Y"
     gene            10695..12113
                     /locus_tag="VLE22_00720"
     CDS             10695..12113
                     /locus_tag="VLE22_00720"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB12945.1"
                     /translation="MKNNTARTPVRVLIVDDEENQRRGLASMIAAWGYEARTAADGQE
                     ALELLAEFPAHILVTDLMMPRMDGSELLRRLSQQGGGPTAIVVTAFGNIETAVSTIHE
                     LGAFWFMEKPIEPRAMRVLLERAAAQSRLSEEAERLQRELSYHGMLGELVGRSAPIQQ
                     VFSLIQQVAPSKAAVLVTGESGTGKELVARTVHALSPRRDGPFVAVNCAAMPETLMES
                     ELFGHEKGAFTGAVERRAGCFELAQQGTLLLDEIGDMPVGTQAKLLRVLEDSRIRRLG
                     GKSEIEVDVRVVASTNKPLEESLRKGEVREDLYYRINVFRVHLPPLRERKEDIAPLCE
                     ALLDSLNRKHGCKITDVQPEVMQAFHSYNWPGNVRELRNILERAVVIAGEGTVSPAHL
                     PVDFGASEPQRRSSEPADPNEVRLRVGTTVDEGEKALILKTLEHTRNNKTRAAEILGI
                     SLKTLHNKLKEYGSAADEAAGE"
     gene            12246..14144
                     /locus_tag="VLE22_00725"
     CDS             12246..14144
                     /locus_tag="VLE22_00725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB12946.1"
                     /translation="MSLKTRLRLSIVALVVSIVLALSALNLHSVATARSEDVLERARM
                     SALQVQSVLVQRLNEQTVKRTPPANLDETKALWSEIVRSDQELTSLLKDTMASSRTTV
                     EIAISGEDGRILTSSNPATVGTPARKLPSLENWTRKSPWEKLWDIFTQRLDYEVRLPL
                     GVAEQQAPVFEIQVAVSSVLLRNTLVPQMWYFALALLVSFLGALALAMLVSNLAFRPL
                     AKISDAIDRIARGESVRDDAPGEGESKEYTALQSKLSVLGQQFRGAREDAVQLRGNIE
                     RLLERLEEVVLLFDRNDRLIVAGRSAENFLGRGRWEMFGRGLDEVFPSSTPLGAVIQS
                     AVQLRHPVKDHLVQLDRSGQAAARLLVNVELLEDFPSHERLGTMVSLRDAESRRQLQS
                     QLDVSTRLEAISRLTGGVAHEIKNPLNAIALHLEVLKSRLGSDAELEQEIGIISSEIT
                     RLDRVVKTFLDFTRPVDLRLATVDLGKLVGEVATLVEPQARTCAVEVVADTGHEPARI
                     RGDRDLLKQAVLNVVMNGVEAMKNGGRLEIQVRRSGEDCTVRVSDQGPGIPPETRGKI
                     FNLYFTTKGKGSGIGLAMTFRIVQLHNGTIDCTSETGKGTTFWLRFSAAGEEETAGKE
                     PAAAVEES"
     gene            14172..14762
                     /locus_tag="VLE22_00730"
     CDS             14172..14762
                     /locus_tag="VLE22_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12947.1"
                     /translation="MKNQRLWALLILLTLVPAMTSCWPFRKKKPVVPAPPPPPHQQTV
                     PKPVQFPPPPAIEQGPSEPPIPEEPHEAIPPPPQKKRPSPSGPRVQPAPTPQEPEPEP
                     EPPPAPPSPSLQPMLSAQQKQELEAMVHKRITSARLSLASIQNRSLNPEQKAAVNQIR
                     IFLEQAEDARKTDLIRANNLAERADVLARDLVAGLR"
     gene            complement(14857..15609)
                     /locus_tag="VLE22_00735"
     CDS             complement(14857..15609)
                     /locus_tag="VLE22_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB12948.1"
                     /translation="MRFATGIFPVAVVALAVTISSGCATKKFVRTTVSPLEQRIGELD
                     KKTGEQGNSIEELERGVSRADERAMSADAKAAAAATEAARANEQASLAGKSATDARSL
                     AERGLGRADQAHQKIENIDNYKLIFEENVLFNFGKSELTDEARSKLDEAAGKVESTKH
                     FVIEVQGFTDKTGPDSHNLELSRRRAAAVVRYLTMEHKIPLYRVHTLGYGSESPIADN
                     KTRDGRKQNRRVEIKLYAAEYGDSAQLASNDK"
     gene            complement(15657..16319)
                     /locus_tag="VLE22_00740"
     CDS             complement(15657..16319)
                     /locus_tag="VLE22_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12949.1"
                     /translation="MKHKIFRYLAPLAVVALLGCQSRPPGTPETSTAEPADSAAAETR
                     SVGRPAPERTLEIPAGSVLRVRLDHSVGTEQHQSGDAFTATLDEPVMAGNRVAIPKGT
                     QVTGHVTGAKSSGRLRGRGYMSLTLDSFESGGKAHQLSTTSVGRATGDHKKRNTALIG
                     GGAGVGAIVGAIAGGGKGAAIGAATGAAAGTAGAAATGKKNVVFPAETLLQFELKEPI
                     RL"
     gene            16481..>17849
                     /locus_tag="VLE22_00745"
     CDS             16481..>17849
                     /locus_tag="VLE22_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSB12950.1"
                     /translation="MKPGLLLLIALSAAAADDFVLIRGGEVRPGIRIDEFEILDHPVT
                     NGEYALFVAGTGHPAPLHWSNGRIPPGMETWPVSFVNRYDVAEYLKWRSQKEDRLHRL
                     PTAMEFEWAARAGAANVKYPWGDAAPDGKANYDPSGTRTFAEWRQYLKPVKSYPPNSW
                     GLYDMAGNVWQMVDTYPDPATSRFVYRITSPVEREGALAGGSWARAEYYLRCGITGNA
                     SSGIRHPDIGFRVVREPVGATHFQRQSRRLIATPAGNAAVFLSWQLLPGDPPDAEFHV
                     YRTLRRDASGVRVTSQPVMNSTSFIDRDAPPEGRVYYRVRPVIRGGKPGLPSEWAGVE
                     PGRPRTGLIAVFQPTAQSGGFTPVFGDLDGDGTLDAILRLNNGIQEMSRDPGVPVELE
                     AFTSYGRSIWRRPLVRHDQCFGSSSNVPVVVYDLDGDGRAEVIARAAEGDSVYLAVLD
                     GMTGRI"
BASE COUNT         3330 a         5809 c         5266 g         3444 t
ORIGIN      
        1 agtcgccttc acgagcagcg tcctgcgatt cgccgtggcg ttgggctcga tgatcctcgt
       61 gaatgccatg atgtaccggc tcggcccgaa tcgcccccag cgatggcgta acgtgtggcc
      121 tggggccatc gtcgccaccg tcctctggtg ggtcgcaacg cagggtttcg cctggtacgt
      181 gcgcaatatc gccgactaca acctgctgta cggaagcatc ggagccgtaa tcgccctgct
      241 cgtgtggatg tacgtgctgg ccgcgatcgc cttcatcggc tgcgagttca atgccgagta
      301 cgaacggatg gtcgctgcgg ttcgcccggc ggcgccggct aatacggtgt ctcgttgatg
      361 gccttcacca ttgcccagaa gttttccatc ggcgtatcga gttgtacgtg atgcgtcggc
      421 ccgaggatca gcccgccgcc gtcgcctacc gtacgtagcc gctctgacac ctcggctcgg
      481 acgtcatccg gcgagccgaa cggaagcgtc tgctgctcgt cgactgttcc ccagaaacag
      541 aggcggctac cgaactcgcg cttcagccgg gccgggtcca tcgacgctgg ctggaccgga
      601 ttcagcacat ccacaccgac ttcgatcaac tcggggatga tcggatagat gcacccgtct
      661 gaatggcagg cgatcttgag gcggggattg atgcccttca gcgtggcgat gaagctcgcc
      721 atcctcggct tgaggaagca ccgccagtgt gcgggcgaaa tcagcatcgc gttctgcgcg
      781 ccaacatcgt cgccgatcca gatcatgtcc acgcccatcc gcacgagcgt cttcgctgcc
      841 gtcagatgat aacggtatgg gatctccaga atactctcgg cgagatcggg gtcctcgagg
      901 aaatcgatca tcaactgctc gtagccgcga agtgcccacg cagtttcgaa gacagtcgta
      961 accgtcactc cgacgatcca atactcgctc tgcgattcac gcagcagcca ctcggcctcg
     1021 gcgtataact ccgggcggga tggatcgggt ggtcggtagc ggctgagcgc ggaggcgtcg
     1081 gccagcggac ggcccaccgt ctcggtgtaa tatccggcgc cgtacggcgt ctcatacggc
     1141 atctgtttcc aggtgatacc ccactcgtcg acgtagcgtt cgccgtcctg gtagtagcag
     1201 ttggcccatc cgacggaggt cagcagcatg tcctcgccga ttgcgtgctc caattcgtag
     1261 gtatttccgc ctccatgtgg attgtgcgtc ttgcggccct tgatctcgag atcggcgcgc
     1321 agccggtccg cgaattccgg cgtgaagctg atctgcatcg ggcagcggtc gggcggttcg
     1381 tgactcaacg ccgcgatcac gcgttccctg tgtgtcatcg cgtcacctcg cttgtcattc
     1441 gacccacagc acttcatacg gcttcagctc cacggtgccg ctcatcttcc gcccgctgat
     1501 gcgttccgac acaccgcgca cgaactgcac cgcgccgggg cgcggcgtcg tgttgatgac
     1561 aaacaggctc ccatcagatc gcggcacggc ctggaagccg gccggacacg gacccgccaa
     1621 cagcccctcg gtcggaggcg ccaattgctg gaagagccgg acaacggaat cgtcattcgg
     1681 ccaaaacgcg agcttcacgg cagcgccttt gccaagcctc ttccgcgtag ccgctggctg
     1741 ggagtcgagc accggctcgg tgcttcggaa acggccgatc acctcgacat ccgggtccac
     1801 cttaaggtac tcgcagtacc catcgatgcc aacgggtgac gtcccgccga gccagtcgac
     1861 ctgctggtcc atgcggcccg tgtcccgtga cgtgcccatc cgccgggccg tgcgcgccac
     1921 cgctccggtg atcgcggtca gattcgcgcc gaagccatcg tttcggaaga taccgtccga
     1981 cgactggtag gcggtgaaag ggctcaccac gacgtttccc ccgttccgta cgtatgcctc
     2041 cagagccctc atgagccccg tatggtctag agccgtagcg gtgggaatga ccaccagcgt
     2101 gtacttgccg agccggtcgg gcttcgatgc atcctccacg ctgacgctgt ccactccgat
     2161 tccgagccgg tgcagcgcgt cgaaggcctg ggggagcacc tgccggctgg acggcacgtg
     2221 cggatatgtg atgagcgccg cccgctgatc gaagtcagtg atcaccgcgg ccgccgtttt
     2281 cacagggtgg cgcaacagcg ggccgctcca tttgtcgaag aactcgccca gttcgaccag
     2341 ccagtggaaa tccggctctg gtgcgcccgt ccagtcgagt atgccgcccc agtgcggcca
     2401 gtgccccccg tgccagcggt ttcccgtcca gtacatcagt ccgaaggcgc cgtatgccac
     2461 cggctggagc atccacatgc ggaattgttc cttcgtcggg aaggggtccc acatcaccgt
     2521 gtcgccggcc acgccgaccc gcgtttcggt cacaaggaac gagcccagac catgcgcgga
     2581 gcggtgcatg tcgaggtgcc agccgagcgt ggtccagaag tccggttcgt ccgacgacgg
     2641 ctggtagaag ttcaggcccg cgatgtcgag cgactcccgc gcgcgaggat cgtcggcaag
     2701 cgcctgccac atcgtgttcc agtcggtggt gatccaatgt ttcacaccgg cttcccggag
     2761 cgctttggtc tgctcggcaa agaattccag aatcgagtcg cgctggaagt ggagatgagc
     2821 cagagacagc gccggcaggc cggagcggcc cgcttgcggc atctcgaaga ccgtccttgg
     2881 ctgaggcacg tcgtccaagg agtcgacctc catcccccag ctcaccagtc gcagcctgcg
     2941 gttgaattct tcgggggtgc gataagtctt cttcagccag tccttccacg cacgctcgca
     3001 ggctgagttg tagcacggcc ttccgacgag aaactcgatc tcgttgtcga gttgccaacc
     3061 gatcaccgcc gggtggctct tgaactcgcc tccgagagcg agcaggtagc gcctcagtgc
     3121 ctgacgatac aggggatggt tcagacacgc tgccttccgc cccatcgggt gaagcttctc
     3181 ccccggattc aattccacga gcatgtccgg atttttggcg ctcagccact gcggggcgat
     3241 gtaactggac gtcccgagca ctgccttcat gccccgcctg tcgagatcgt tgaggaactc
     3301 cctcagccag ccgaagttgt accggcccgg cgcagtctcc aggttccccc atgaggattc
     3361 gctcacgcga accgccttga agcgcgcctt ctggaatacg tcgagcatcc ggttccattc
     3421 ttcgcgcgtt tgcagctccg ggtacacgct cgcgccgaag aggaactcgt cgggaacgat
     3481 ccgcagcggt cccgccgcga gcggcataga cagcaacagc aagattgcga ctgagaggtt
     3541 cacgactcta caccctaccg acgcggcatt ctatcgcaaa cccggatgtg ttgcgtcccg
     3601 gcttcctata ctgaaagtat ggccttccgt ctcgccgtcg actatcgccc ccgaggcgat
     3661 caggaagcgg ccatcgaaca gcttcttcga ggagtgcgcg acggggagca gcatcaggtg
     3721 ctgctcggtg tcaccggctc ggggaagacg ttcaccatgg ccaaggtcat cgagcaggcc
     3781 ggccgcccgt cacttgtgct ggcccacaac aagacgctgg cggcgcagct ctatcacgag
     3841 ttcagaacct tcttccccgg caacgccgtc gagtacttcg tcagctacta cgactactac
     3901 cagccggaag cctacatccc gtcgggcgac gtgtacatcg agaaggaagc caccatcaat
     3961 gacgaactcg acaagctccg gatgtcggcc acccggtcgc tcttcgagcg ccgtgactgc
     4021 attatcgtcg ccagcgtaag ctgcatctac ggcctcggtt cgccggaagc ttattacggg
     4081 atgttgctca tgctcgaaaa gggccggcag attccccggc aacaaatcct gcagaaactc
     4141 gtcgagatcc agtacgagcg gaacgatgcg gacttccggc gcggaacgtt ccgcgtgcgc
     4201 ggcgacgtga tcgaggtcta cccgacctac gatgactatg cctaccgcat tgaactgtgg
     4261 ggtgatgagg tcgagagcct ttcgcagatc gacccgctgc tgggccaggt gcggcagacc
     4321 tatctccgtc tgcccatcta cccgaaaacc cactacgtga tggacgaaac cacgcgagag
     4381 aaggcgctgg cgtccatcga gcaggagctg cgctggtggc atcccgaact ggaaaagcag
     4441 ggaaggctcg tcgaagcgca gaggctttgg cagcggacga tgttcgatct cgagatgatc
     4501 cgcgaaatcg gctactgcca cggcattgag aactattccc ggcacttctc cggacgcctc
     4561 cccggtgagg cgccgccgac gctgctcgat tacctgccgc atgactcgct gttgttcatc
     4621 gatgagagtc atcaaaccgt cccgcagctc cgcggtatgt atcacggaga ccggtcccgc
     4681 aaggagacac ttgtccagtt cggtttccgt ctccccagtg ctctcgataa ccgtccgttg
     4741 accttcgagg agtttgagca ccgggtcaac caggtggtct atgtgtcggc aactcccggt
     4801 ccgtacgagc tgacgaagtc ggccggcgtc gtggcggagc aggtcatccg cccgacgggt
     4861 ctcgtcgacc ccgaggtcga ggtgaggccg atccgcggtc aggtggacga tctgctgaac
     4921 gagatccgca agcgttcgga gtcgagcgag cgagttctgg tgacgacgct gacgaagcgg
     4981 atggccgaag atctctcgga gtactactcc gaggtcggag tgaagtgccg ctacctccac
     5041 tccgaagtct cgacgctcga ccgcgtcaaa atcttgcgcg acctccggcg gggcgagttc
     5101 gacgcgctga tcggagtcaa cctcctccgc gaaggccttg atctgcccga ggtctctctc
     5161 gtggctatcc tcgacgccga caaagagggt ttcctcaggt ccgcgggctc gctgatccag
     5221 accgtcgggc gcgccgcccg gaacatcaac ggcaaggcga tcctctacgc cgatgtgatg
     5281 accgactcca tgcgtaaggc gatcgacgag accgaccgcc ggcgccgaat ccagactgag
     5341 tacaacgaga tcaacagcat caccccgcaa tcgatcatca aaccgattga catgagcctg
     5401 gtggccgtcg ccgagggtga ctacgtgacc gtgccgctcg aggcggaaga cgaagaggtc
     5461 gaagccatgg ccccagagca gcggcagaag ttcgtcgagg aactcgaaga gcgcatgcgc
     5521 gaggccgccc gcaagttcga gttcgagcgc gcagcacaga tccgcgaccg catcaaggcc
     5581 gtcaaatcaa tcgacgtcct cgaaccgagc ggaactgagt agggcgcccc cgcccaacta
     5641 caccacctgc tccccggcgc gcagaagcca cgccgaggga tgctgcgcga agccgatttt
     5701 cggatagtat cccaccgcct tcggcgccgc caatagcacg atatacaccc ccggcccgct
     5761 ttcccgctgc gtgatccgga tcagctcctt cccgatccct tgattctggt gcgacactcg
     5821 caccgccagg tccgagagat acgtcacgta ggcgaagtcc gagagcgagc gtgagatgcc
     5881 taccaacaga tcgccgtccc acgcggtcac cacgaggttc gcgttgcgga gcatctcccc
     5941 cattcgctcg cgctcgtcta cgggccggcg ctcgccgagg gtggaggcgc ggtaaagctc
     6001 gatgacctga tccaggtcga agttatttcc ggtgcgatac gtgatcatgg agtgaagccg
     6061 aagcttcctc cactttactt cggccgggcg tcctccggct ttaacagagt cagttccttc
     6121 tgctcccggc ctttctcgct gaccgtcacc ttcaccgctt tctcctcgaa gggcctcagc
     6181 gcgtcggggt cctgtagcac atctgtcagc gactcggccg acgcgtacaa cttgtagtcg
     6241 ccgggagcca cccctttgat cgagaaccgc ccgttctggt cggtgttggc ccatttcgtc
     6301 tgggacggat cgtcagctat gggcgggtcg ggcatcagca gcacagaaat cccctgcgcg
     6361 ggcttctcgt cgtcggtcac caggccctcg actgttccgg cattcgggct gagcacgagt
     6421 tcgatggagg tgctgccctg cgcttggctg aggtccacgc tcttgtccgg cgcgtcctgg
     6481 tttccgaaac ggaccgactt caggtacgtg ccctcaggca ggttgtaagt cccaatcgag
     6541 taacggtccg gcatcacgcc gtccatccgg aaggttccgt cttccttcac cattgcgttt
     6601 tgcataccga aaccaatccc ttccgtcggc cggagcatca gccggacccc gtgaatctcg
     6661 attttctcct tcccttcgac tcgcacgaag ccgttgagcg tcaagccttg gctgactcgt
     6721 acgaccacac cttcgacgtc ggcctcgccc acatccaccg gcgcccgccc gaccacctgt
     6781 ggccggccgt ctccgcgcag catcaggctc aggttgtagg acccgggctg caccttgccg
     6841 atcgtgaacg tgccatcagc attggcccta gctccgccgc cgccgaagaa catgaccatg
     6901 gccttgcggt cccgcgggga cagcgacagg ttcatcatct tcagttgccc cgcggtcgcc
     6961 ccggacacac tcccggagac ccggtacacg cggctctttc gcagcgtgat attgatcccg
     7021 gagacctctt ggcccggccc gacctccact gcggacgcgc tcgccgcatc ggtcacgccg
     7081 gggtagaagg tcggcacatg tgcctcgcga acctcgtcgc ttttctccac gccttcctga
     7141 cgaccccaga agtctggggt gggattggcc tgaacaatgt accgtcctgg ggcgagatcg
     7201 gcaacgcgat aatctcccac gtcgttggcc tgctccccac ccatcccggc gccgcgaccg
     7261 ccgccggatt gaacttgcct cgtgacattg atgaaggccc cccggaccgg ctcaccctcc
     7321 tcgtcgagta ccttgccggt aatcacgcct tgcggcatga gcttgaactc taggtccttg
     7381 agttcctgac cggtcatcag cgttaaggcc gtgccgctga agccgtatcg tcgagatccg
     7441 tacgattgcc gcagaaagcc ggcacgttcc gccgtcaact ggtaggtgcc tggctcgatg
     7501 tcatcaatgg aaaacttgcc ctcggcatcc gaaacggcgg aatacgactt cgattccatc
     7561 ctcatctggt gcaggatgag tttcacctta cggaccggtt cgcccgtttg agagtgcaaa
     7621 gtgcggcccg taatccgaca caacttttct tttttctggg tctcatacgc ggcgctctcg
     7681 gccgccggcg gtggctgctg cgcacaagca agggcagcag aaagaacgag aagaagcaag
     7741 gacggggtat gcatctgcgc gttgccctct agccgaatta taaccacgca gtcttacgga
     7801 gtctgtgcat caccagactt cgcaagctcg atctccaccc gttcccggcc tctttcggca
     7861 accgtcacct tcacggcttt ctcctcgaac ggcttcagcg cctccggatc ctcgtcagcg
     7921 ccactgagtg attctttcga agcgtacagc ttgtactcgc ctggaaccac tcctttgatc
     7981 tcgaaccacc cgttctggtc gcagtttacc cacttccact tatacggctc ctctggcttc
     8041 ggcgggtcgg gcacaagcag cacggaactc cccggcgccg gcttcccatc gtccgacacc
     8101 aacccttcca cggttcccgc atacgggctc agtatgagtt cgatggatcc gctgccttgc
     8161 gtctgactca ggtccacgct cttgtcaggt gcctcgtgat ttccgaaacg gacagacttc
     8221 aggtaagcgc cctccggcag gtcataaacc gccaccgagt accggtcggg cattacgcct
     8281 tcaatacgaa acgtcccatc ttctttgact atcgcggtgt atgggaaggg ctctccgtcc
     8341 tctaccggtc gcagcgtcag acgaagcccg tggacttcgg ctttctcctt cccttcgaga
     8401 cgcaccagac cactcagcac cacgccctgg ccgacctgca atatcacgcc ttccacgtcg
     8461 gcctcggcga cgtctaccgt agtccgtccg acgacctgag gccggccgcc ttctccaagc
     8521 atcaggacga tgttgtatga tcccggctgc acatttgcca acgtgaaaga gctgtccgga
     8581 ttcaccctgg gcgaaacgta gctggacacc ataggtctca ttctgtgctc gcgcgacctc
     8641 accgacacgt tcatcatctt caactcgctg gcagtggcgc caatcacttt gccggaaatg
     8701 atgtagacgc ggctcttacg gagcgtgatg ttgattcccg agacctcctg gccggggccc
     8761 acctccactg tggccgcgct cgcaggatcc gtcactccgg gatagaacgt cggcacatag
     8821 ctctcacgaa cctcgtcctt ctttggtttt gtagtttctt cgctacggaa gtcgtactcc
     8881 gccgcggcct gaacgatgta ccgccccgga gcgagattgg cggcccggta ctcgccaacg
     8941 tcattggttg attctccgct tgcacctggt gtgcgtccgc gtccgccggc gtaaacctgg
     9001 cggaagacat tgatggacgc ttcttggacc ggttctccct cctcgtcaag caccttgcct
     9061 gtaatcactc cctgcggcat gagctgaaac ctgaggtcat cgacctcctg gttggcggtc
     9121 agattcagtg gtgtcgggct ataccctgat ccgcgggaac tgtaggctcg gcgcaagaaa
     9181 ccggagcggt ccgccagcaa atagtacatg cccggctcga ccttctcgat cacgaagcgg
     9241 ccttctgcgt cggaaaccgc gcctaggttg cgcgaatcca tcaccacccg ccgcaggatc
     9301 aatcgcactc tagggaccgg ctcgcccgtc agcgcgtgca cggtgcggcc tgtgatccgg
     9361 cacagctttt ctttcttctg ggtttcggga gcggcgctct cagctgccgg cgggatctgc
     9421 tggacacaac cgagagcagc aggaagaacg aggaagagca agaaagcaat ccgcatctcc
     9481 gcgcaacgct ccaacggaat tataaccgct cagcgatccg gaggcagctg aagcggatca
     9541 atacccagcc tgcttgtcca cgacgttcag caacggctca ccggccacaa accgtcggac
     9601 attttccttc accagcgcca cgcgccgctc ctgaatgcgg tcggacgtgc ctgccgtgtg
     9661 cggtgtgatc accacgttct cgaatttcca cagcggatgg cccggaggaa gcggctcggg
     9721 gtccgtgacg tctaggcctg cgcccgcgag gcgcttgctg tcgagcgccc gcaccagtgc
     9781 gttgttgtcg aaaatctttc cgcgcgagac tgctacgaag tacgatccct tcttcatcag
     9841 gtcgaactgc cgcgacccca ccatgccgcg gctcttcggt gtatgcggag cggaaatgaa
     9901 tatcacgtcc gcctcaggca acacctcgtc cagccgctcg ggtgtgacca tcttctgcac
     9961 aaaaggcata tacggcttgt ctttcggatc aacaccgatc accctcatcc caaacccgtt
    10021 cgcacggacc gcgatctgcg ttccaattcc acccacgccg atcaccaccg cggtctttcc
    10081 gttcaattcc accattccct gctctgttcc ccgtttccac tgttcgctgg tgcgtgcggg
    10141 aatcgtgcgg tacaaaccgc gcgtcagagc gagcagcaac gccatcgcgt ggtcggcgat
    10201 ctcgggaccc tggatgatct ttccgttggt caggacgatg tcgctgttca ccagttccgg
    10261 gctgaggacg ttttccatcc cggcgaaata catctgcacc cacttcagtt tcttcccagc
    10321 gcgtacgaga tcggcgttta tggtccccag tagcgcgtct gcatcggcga tttcgttctt
    10381 caactgggcc gagtcaaccg ctttaacggt ggctccaggc gctaccgcct gaaattcggc
    10441 gaccgcttcg gatggcatcc cggtgaccag gatcttcttg ccctgcgcaa agacactgcc
    10501 agcgcacgca agtgtggtaa tggcgagtag tgacaaatgt ttcatggccg gacctcctac
    10561 tttcggcgaa ttgtatcgca gccacgttgg ggtctgccgg cgactttcgt ggcggcatcg
    10621 tctttgctct cagtcatgca cacggaaagc ggcctggaca agggtccctt cgcggttaag
    10681 tacaataaca ggcaatgaag aacaacaccg cacggacgcc cgtccgggtt ctgatcgttg
    10741 acgatgagga gaaccagcgg cgtggtctcg cgagcatgat tgcggcgtgg ggctacgagg
    10801 cccgtaccgc cgccgacggg caggaggcgt tggagttgct ggccgaattt cccgcgcaca
    10861 tcctggtgac ggacctcatg atgccccgca tggacggctc cgaacttctg cggcggctct
    10921 cgcagcaggg aggcggcccg acggccatcg tggtcactgc gttcggcaac atcgagacgg
    10981 ccgtttcgac aatccatgag ctgggcgcgt tctggttcat ggaaaaaccc atcgaacccc
    11041 gcgcgatgcg cgtccttctc gagcgcgcgg cggcccaaag ccggctgagc gaagaggcgg
    11101 agcggctcca gcgggaactg agctaccacg ggatgctcgg cgagttggtg ggccgctccg
    11161 cgcccatcca acaagtcttt tcgctcatcc agcaggtggc gcccagcaaa gctgcggtgc
    11221 tcgttaccgg cgagagcggc acgggaaagg aactcgtcgc gcggaccgtt cacgccttga
    11281 gcccaaggcg cgacggtccg ttcgtggccg tgaactgtgc cgcgatgcct gagacactga
    11341 tggaaagcga gctgttcggc cacgagaagg gcgctttcac cggtgcggtg gagcggcgcg
    11401 ccggctgctt cgaactcgcg cagcaaggca ctctcttact cgatgagatc ggagacatgc
    11461 ctgtgggcac tcaggcgaag ctgctgcgcg tgctcgagga ctctcgcatc cgacgactcg
    11521 gtggcaaatc ggagatcgag gtcgatgtgc gcgtcgtcgc ctcgacaaac aagccccttg
    11581 aagagtcact acgcaaaggt gaggtccggg aggacctgta ctaccggatc aacgtgtttc
    11641 gcgtccacct tccgccgttg cgggaacgca aagaggacat cgctcctctg tgtgaggccc
    11701 tgctcgactc actgaaccgc aagcacggat gcaaaattac ggacgtccag ccggaagtga
    11761 tgcaggcatt ccatagctac aactggcctg gcaacgtccg ggagcttcgc aacatactcg
    11821 aaagggccgt tgtgatcgcg ggcgaaggga ccgtcagccc tgcccacctg ccggtggact
    11881 tcggcgcgtc cgagccgcag cgaagatcgt cggagcccgc cgacccgaac gaggtgcgac
    11941 tccgtgtcgg aaccacggta gacgagggtg agaaggcgtt gatcctcaag accctcgaac
    12001 acacgcgcaa caacaagacc cgggcggcgg agatcctcgg tatcagcctg aagaccctgc
    12061 acaacaaact gaaggagtat ggctcggcgg cggacgaggc ggccggcgag tagaaacgga
    12121 attcggattg caaggacgaa tggtggagac ggaaccgcga ccgtcagggc ctacgaaaga
    12181 tctcggcccg tcaggaagcg gtcatccgcc gcaccgcgaa cgtcacggag cggccgtcaa
    12241 gtgaaatgtc gctgaaaacc cgcctgcggc tgtccatcgt cgctctcgtc gtgtccatcg
    12301 tgctggcgct gtccgcgttg aatctgcata gcgtggccac ggcgcgatcc gaggacgtgc
    12361 tcgagcgcgc gcggatgagc gccctccaag tgcagagtgt tctagtccag cggctgaacg
    12421 agcagaccgt gaagcgcacg cccccggcga acctggatga aacaaaggct ctctggtcgg
    12481 aaattgttcg gagcgaccag gaactcacca gcttgctaaa agacacaatg gcaagctccc
    12541 ggacgacggt cgagatcgcg atttcaggtg aggatggacg catccttaca tcctccaacc
    12601 cagccacggt cggcacaccg gcgcgcaaac ttccgagcct ggaaaactgg acacggaaga
    12661 gcccatggga aaagctctgg gatatcttca cccagcggct cgactacgaa gtgcggctgc
    12721 cgctcggcgt ggcggagcag caggcgccgg tgttcgagat acaagtggcg gtttcttcgg
    12781 tgctgttgcg aaacacgctg gttccgcaga tgtggtactt cgccctggcc ctgttggtat
    12841 ctttcctggg cgctctggcg ctggcgatgc ttgtttcaaa cctggcgttt cgtcccctcg
    12901 cgaaaatcag cgacgccatc gaccgcatcg cgagaggaga gtcggtccgg gatgatgcgc
    12961 cgggcgaagg cgagtcgaag gaatacaccg cgctccaatc gaaactcagc gtgcttggcc
    13021 agcagttccg gggcgccagg gaggacgctg tgcagttgag gggcaatatc gagcggttgc
    13081 tcgaacggct cgaagaagtg gtgctcctgt tcgatcggaa cgaccggttg atcgtggccg
    13141 gccgatcggc ggaaaacttc ctaggccgcg gaagatggga gatgttcggc cgcgggctgg
    13201 atgaggtctt tccttcttct acgccgctgg gggccgtgat tcagagcgcc gttcagctcc
    13261 gccaccccgt caaggaccac ttggtacagc tcgacaggag cggccaggcg gctgcgagat
    13321 tgcttgtaaa cgtcgaactg ctggaggact tccccagtca cgagcggctt ggaacaatgg
    13381 tgagccttcg cgatgccgag tcgcgccggc aactgcagtc ccagcttgat gtctccaccc
    13441 gcctggaagc catcagccgc ctaacgggcg gcgtcgccca cgagatcaaa aatccgctga
    13501 acgccatagc gcttcatctg gaagtgctga agtcgcggct ggggagtgac gcggaactcg
    13561 agcaggagat cggcatcatc tcgtctgaga tcacccgcct cgaccgcgtc gtgaagacct
    13621 tcctcgattt cacccgtccg gtcgatctgc ggctcgcgac cgtggatctc ggcaaactgg
    13681 ttggcgaagt ggcgacgctt gtggagccgc aggcgcggac atgcgcggtg gaggtcgtcg
    13741 ccgacacggg gcacgaaccg gcccgaattc ggggcgaccg cgacctcctg aagcaggccg
    13801 ttctaaacgt cgtgatgaac ggcgtcgagg cgatgaaaaa cggaggcagg ctcgagattc
    13861 aggttcgccg ctcgggggag gactgcacag ttcgcgtgtc cgaccagggg cccggaattc
    13921 cccctgaaac gcgcgggaag attttcaatc tctatttcac gacgaaaggc aaaggctccg
    13981 gaatcggcct ggcgatgaca tttcgtatcg ttcaactcca taatggtacg atcgattgca
    14041 caagcgagac tggtaaaggt acgacgttct ggctacgctt ctctgccgcc ggggaggagg
    14101 agactgctgg taaggaaccg gccgccgccg tcgaggagag ctaagttacc ggacgggcgg
    14161 tgtcgactgc aatgaagaac cagaggctgt gggcgctgct gatcctgctg acgctcgtgc
    14221 cagcgatgac gtcctgctgg ccgttccgga agaagaagcc ggtcgtgcct gcgcctccgc
    14281 cgcccccgca ccagcagacg gtcccgaagc ccgtacagtt tccgcctccg cccgcgatcg
    14341 aacaagggcc ttccgaaccg ccgattcccg aagagccgca cgaggcgatt ccaccgcccc
    14401 cacagaagaa acggccttct ccatccggcc caagagtgca acctgcgccc acgcctcaag
    14461 agcctgagcc cgaacccgag ccaccaccgg ctcccccatc accctccctc cagccgatgc
    14521 tcagcgctca gcagaagcag gaactggagg cgatggtgca caaacggatt accagcgccc
    14581 ggctctcgct cgcctcgatt cagaatcgct cactcaatcc cgaacaaaag gcggcggtaa
    14641 accaaatccg catcttcctc gaacaggcgg aggatgccag aaagaccgat ctgatccgcg
    14701 ccaataatct tgcggaacgg gctgacgtcc tcgcccgcga tctcgtcgct ggcctgcgct
    14761 gacacacacc catagattcc tggcctggga gtcgcagcag agaaacgccg ctccaacccc
    14821 aggccagtcc gacgactgaa aggatcgacc gtcttactac ttgtcgttcg acgccagttg
    14881 ggcgctatcg ccgtattccg ccgcgtatag ctttatctca accctgcgat tctgcttccg
    14941 gccgtcgcgg gtcttgttgt cggcgatggg agattcgctc ccatatccca gagtgtggac
    15001 gcgataaagc ggtatcttgt gttccattgt caggtaccgc acgactgcgg ccgcgcggcg
    15061 gcggctcagt tccagattgt ggctgtccgg cccggttttg tccgtgaaac cttgaacctc
    15121 aatcacgaag tgtttcgtgg actcgacctt cccggcggcc tcatccagct tcgaccgcgc
    15181 ctcatcggtc agttcgctct tgccgaaatt gaacaggaca ttctcctcga atatcaactt
    15241 gtagttgtcg atgttctcga tcttctgatg ggcctgatcg gctcgtccca gcccgcgctc
    15301 agccaaagag cgggcgtccg tagcgctctt cccggcgagt gaagcctgtt cgttcgcgcg
    15361 cgcggcctcc gtcgccgccg cagcggcttt ggcatccgcg gacatcgcac gttcgtcggc
    15421 gcgcgaaacg ccccgctcca gttcttcgat cgagtttccc tgctcgccgg tcttcttgtc
    15481 cagctcgccg atccgctgtt cgagcggact taccgttgtg cgtacaaact tcttggtggc
    15541 gcaccccgaa ctgatagtta cggcaagcgc cacgacagcc acggggaata tcccagttgc
    15601 gaatctcata cttgtttcct ccaatctcgt gcaatccttt gcgattctcc tcagcctcag
    15661 aggcggatgg gctccttgag ctcaaactgg agcagtgttt cggctggaaa tacgacgttc
    15721 ttctttccgg ttgcggccgc tccggcagtg cctgccgccg cgccggtcgc tgccccaatg
    15781 gcagctccct ttccaccgcc ggcgatagcg ccgacaatcg cgcccacacc ggcgccgccg
    15841 ccgatcagcg cggtgttccg cttcttatgg tcgccggtgg cgcggcctac cgaagtcgtc
    15901 gacagttgat gcgccttccc tccggactcg aacgagtcca gggtcaggct catatatcca
    15961 cggccgcgaa gacgccccga cgatttcgct ccggtcacgt gccccgtgac ctgtgtgccc
    16021 ttcggaatcg ccaccctgtt tcccgccatc actggctcat caagagttgc agtgaaggca
    16081 tcgccggact gatgctgttc cgtgccaacg gaatgatcca ggcgtacacg caacactgat
    16141 cccgccggaa tttcaagggt tctctcgggc gccggcctcc cgacgctcct cgtttccgct
    16201 gccgccgaat ccgccggttc ggccgtcgaa gtttccggcg tccccggggg tctgctctga
    16261 caaccaagaa gtgcgacaac ggcgagcggg gccaggtatc ggaaaatctt gtgtttcatg
    16321 acgttccctc cggaattttt tcctgaatag cgtgcaattt ctgcacgcta ttcactgtaa
    16381 gccattagct gcctgcgatt tatccaaaca cagccactca ataagcacgc cgagtgccac
    16441 gcttgcggcc catccggatg gcttgatatg ctgctcgccg atgaaaccag gattgctgct
    16501 tcttattgca ctgtccgcgg cggccgccga cgatttcgtg ctgatccgcg gcggagaagt
    16561 gcgtcccggc atacgcatcg acgagtttga aatcctcgat catcccgtga ccaacggtga
    16621 gtacgctctg ttcgtcgcag ggacgggaca tccggcgcct ctacactgga gcaatggacg
    16681 aattccaccc ggaatggaaa cctggccggt cagcttcgtc aatcgctacg acgtggcgga
    16741 gtaccttaag tggcgcagcc aaaaagaaga tcgtctccac cgcctgccca cagccatgga
    16801 attcgaatgg gctgcccggg ccggggccgc gaacgtcaag tatccgtggg gcgacgcggc
    16861 tccggacggt aaggccaact acgacccgtc gggaacgcgt acctttgccg agtggcgcca
    16921 gtacttgaag cccgtaaaga gttacccacc taactcatgg ggcctctatg acatggccgg
    16981 aaatgtctgg cagatggtag acacgtatcc cgatccggca acctcccggt tcgtctaccg
    17041 aatcacttcg cctgtggaac gtgaaggcgc actcgcgggc ggctcctggg cgcgcgccga
    17101 atactacctg cggtgcggta tcacaggtaa cgcctcgtcc ggaatccgcc atccggacat
    17161 cggattccgt gtggtccgcg agcccgtcgg cgccacgcat ttccagcgcc agagtcgccg
    17221 cctgatcgcc acgcccgccg gcaatgcagc ggtcttccta agctggcagc tcctgccggg
    17281 cgacccaccc gatgccgaat tccacgtcta tcgaactctt cgccgagacg cctcaggcgt
    17341 gcgcgtcact tcgcaaccgg tcatgaactc gaccagtttc atcgatcgcg atgcaccgcc
    17401 cgaaggccgg gtgtactacc gcgtgcgccc ggtcatccga gggggaaagc cgggcctgcc
    17461 ctcggaatgg gcgggtgtcg agcccggccg gccgcgcaca ggtctgatcg cggtcttcca
    17521 gcccacggcc cagagcggcg gcttcacgcc cgttttcggt gacctcgacg gcgatggaac
    17581 ccttgatgcc atcctccggc tgaataatgg cattcaggag atgtcccgcg accccggtgt
    17641 gccggtggaa ctggaggcat tcaccagcta cggaaggagc atctggcggc ggccgctcgt
    17701 ccgtcatgac cagtgcttcg gcagttcgag caacgtgccg gttgtcgtgt acgatctcga
    17761 cggcgacggc agggccgagg tcatcgcacg cgccgccgaa ggcgattccg tctatctcgc
    17821 tgtgctcgat ggaatgaccg gccgcatcc
//
LOCUS       DATIFQ010000019         6878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2495757, whole genome shotgun sequence.
ACCESSION   DATIFQ010000019 DATIFQ010000000
VERSION     DATIFQ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2495757"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VLE22_00750"
     CDS             complement(<1..245)
                     /locus_tag="VLE22_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12951.1"
                     /translation="MACGALFPLLFFLLLETHAVAQTADGWLAQARRHAQARQWTRAS
                     EAARKAIALDPNLSRPKFYATLAARGDYAAAIPLMEAV"
     gene            complement(335..3631)
                     /locus_tag="VLE22_00755"
     CDS             complement(335..3631)
                     /locus_tag="VLE22_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB12952.1"
                     /translation="MGYSAKRYFLLLVPSLLLLATVDMKGQSTQSTILGTVTDPSGGV
                     IVGATVEVTNEGTSFKRQIMTDQNGDYRVAGLERGSYEVRVTSTGFNTFVRNRIVLES
                     SQIKRVDASMQVGETATTVTVTGDVGLLETETAALSNVKSTQDFVQLPQSVYGRGWPN
                     VVFVTAGVQSGGVDGCCGVVINGARDTANNFTSDGIVVNDVVSSRQTANGFPGGDIES
                     YQEFKVMTANNSAEYSQVAQMSMISRSGSNGFHGSFYWGNFNSRFNARAWNDPTKPSF
                     ENHNMFAINNGGPVYLPKIYDGRDKTFYFFHYSGARYRTGSRTRTSVPPPAFRQGDFS
                     ALLGQITIVDPLTGVPFNDNKIPGDRISSVSRAVQDLVYPDPNLTGLGDFGITENFYA
                     DPGYKFNADGLSTRVDHKITNSNTLFVRVGLTIHNHDVIKGAMKDGYGSSSFYGNVPG
                     RTVVISDTHTFSPTLVNEGKLGYSRNGYFGSDYNFGKQPDLGIEGIDNPANDPAIGGL
                     PNFSFSGAIPFESTGTWVNSYASVQNDYQAVDNLSWYRGRHTFKMGADVRRYQVNDVN
                     NPTSLRGAYNFDDRLSGLAYANFLLGFPSYAERAIARPSAYLRSTQVGFYFQDDFKIN
                     QSITFNYGLRYEYQSPWVDKYDRLFTFDPSTGSLVTAGTSIPTDLVPAVAATLPIKSA
                     SEAGFPTRSLMEKDSNNWSPRFGLAIRPFADATTVVRLGYGLYSQLWPGSLGLNATGG
                     PWQSTESFFIEGNQPSIRFPRPFVTTSEFSGLQGVAGLSVKFPNERTQQWNVSVGRQI
                     WNTAVEIGYVGTKAKNIPFVQDLNLLHPSTEPYSSARRPYQRFNAVSLTQSGGSSIYH
                     GLNIQADRKMSGGLSLNANYTWAKALTDVALSNYAQSYSQNQYSRYLERGDDDWVRRQ
                     QLRFSYIYELPFGRGKRFLNGLASAPNYVLGGWQFSGITTMVTGRRLSPSFSGTDPAN
                     TNQFGGRPDRIGDGNLDSGGMRDRIKGGSPIFDSEAFVQPVDGRGYYGNSARNILTGP
                     GGHSWNMILAKNFQLKEQARLQFRWEIFNLLNRANFSNPSTNISGGDFGLVTSAGNMR
                     KMLFALRLDY"
     gene            4056..6722
                     /locus_tag="VLE22_00760"
     CDS             4056..6722
                     /locus_tag="VLE22_00760"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HSB12953.1"
                     /translation="MDSKAGWNRRRLLQAVGASVPTLKLVGVAASAAQGDGAKFTPID
                     LSPYFNASSAEFGPRGMGRQTGAPGARGDVLIRPPGGKQELQGIPFLLGPEDVQQKSW
                     IAVSTAGQPWAAPKIEIPIGHTAPCLCLAQFCDWDPNEIDQSSFESIEHLGQKLAEAT
                     LVYEDGSEFSAPIRRRLEVHAPSISFGYQCFLAMPSSTWEPAKLTDPLPNGLGWGNLQ
                     TVAKGKSPGQAPLWIWALENPNPDRKVKALRVRAVAEDPVVLCGLTLFHGREHPLRRE
                     RRTLYRITLPSPTAEEKGRWTVEVDLGVVVRTYVLPEFDPESWLRADGAGFGERSAEP
                     SQSRHLYAEIAASADATLEVRDTASGGRYQFDLIRAVPGQELEARRGSSRIEILEREK
                     TWIHGQVVDSATGRPTPVRLAFRSKAGRYIPPYGHRTEINGGWFQDYAADAKISSNSY
                     AYVDGTFQAELPVGELYVEISKGFEYKAIREKLEIQPSQRELKLQIGKHADLRSKGWV
                     TADTHVHFLSPSTAVLEAQAEGLNLVNLLAAQWGDLYTNVGDLPFGPLTSPDKETVVW
                     VGTENRQHLLGHIGLLGGRGEPVFPMSADNPSEAFIGDPLWSSMAEWADACRKREGVV
                     VAVHFPNPFAELAADIVLGKIDAVELYPQGDGGFRTRGYNDWYRYLNCGYRVTAAGGT
                     DKMGTYTPVGGNRTYAYIGQEQFDFSSWAKAVRAGRTFASSGPLLLFQADGRMPGGEI
                     PMRKGEAAVEVSVEANSHVPFHRIEIVHNGRVVASREEKAGTKRMAFSESVKVQAPGW
                     LTARCSSTLGPSLSARFGIAAHTSPVYLSVAGEEQFSEPALTYLLTLVEGTRGYVEKL
                     ATRPDTESFARVLKVFADAREHLHRRLHEQGR"
BASE COUNT         1445 a         1933 c         2123 g         1377 t
ORIGIN      
        1 acggcctcca ttaaggggat ggcggcggca taatccccgc gcgcggcgag ggtggcatag
       61 aacttgggcc tggagaggtt cgggtcgaga gcaatcgcct tgcgggctgc ttcgctcgcc
      121 cgtgtccatt ggcgcgcttg cgcatgccgg cgcgcctgcg cgagccaccc gtcggcggtt
      181 tgcgcgaccg cgtgtgtttc aagcagcagg aaaaacaaca gcggaaagag ggcgccgcaa
      241 gccatcgtct tccatgccct acgagggtgg cagacgacca acggcgatcg tccgccccac
      301 gagacaaaag gcacagtatt tctaatgcac aactctaata gtcgaggcgc agggcaaaca
      361 acatctttct catattgccg gcgctcgtca ctaagccaaa gtctcccccg ctgatattgg
      421 tgctgggatt agaaaaattc gcccgattaa gcagattgaa gatctcccaa cgaaactgca
      481 gccgagcctg ttccttcagt tgaaagttct tggcgaggat catattccaa ctgtgcccgc
      541 cgggcccggt caggatgttg cgggccgagt tgccgtagta accccgtccg tcaaccggct
      601 ggacaaacgc ttccgagtcg aagatcggcg atccgccctt gattctgtcg cgcatgccgc
      661 cggagtcaag attaccgtcg ccgatgcgat ctggacgccc accgaactgg ttcgtgttgg
      721 cgggatcggt ccccgaaaag gacgggctca accgccggcc ggtcaccatg gtggtgatgc
      781 cggaaaactg ccagcctcca aggacgtagt tgggagcgct ggcgaggccg ttcaaaaacc
      841 tcttgccccg cccgaacggg agttcgtaaa tgtagctgaa gcgaagctgc tggcggcgaa
      901 cccagtcgtc atccccccgc tccaggtaac gcgaatactg attctggctg tagctctgtg
      961 catagttgct cagagcgaca tccgttagcg ccttggccca ggtgtaattc gcgtttaacg
     1021 aaagccctcc ggacattttg cggtcagcct ggatattcaa accgtggtag atggaggagc
     1081 ccccggactg tgttagggag acagcgttga atcgctggta gggccgccgc gccgaactgt
     1141 acggctcagt gcttggatgc agcagattca agtcttgcac aaaaggaata ttcttagcct
     1201 tagtgcccac gtaaccgatc tcaaccgcag tattccagat ctgccgtcct accgacacat
     1261 tccattgctg cgttcgttca ttggggaact tcacactcaa accagcgaca ccctgtaacc
     1321 ccgaaaactc agaagtcgtg acgaatgggc gtggaaaccg tatgctcggt tggttgcctt
     1381 cgataaaaaa ggactcagtg gattgccacg gcccgccggt tgcattcaag cccagggaac
     1441 ccggccacaa ttgcgaataa agcccgtacc caagccgaac aactgtcgta gcatcggcaa
     1501 aggggcgtat cgccagccca aaccgtggac tccaattgtt ggaatccttc tccatcaggc
     1561 tgcgagtagg gaacccggcc tcggaggcgg acttgatggg cagggttgcc gccactgccg
     1621 gcacgagatc ggtgggaatc gaggtccctg cagtgactag ggaaccggtg ctgggatcaa
     1681 aagtgaacaa ccggtcatat ttgtcgaccc aagggctctg gtattcgtag cgcaacccat
     1741 agttgaatgt gattgactga tttatcttga aatcgtcctg aaaataaaac cctacctggg
     1801 tgctgcgcaa gtaggcactg ggacgcgcga tggcgcgctc cgcatagctc gggaatccca
     1861 gtaagaagtt cgcgtatgcc aagccgctga gccgatcgtc aaaattatac gcacctcgca
     1921 atgaggtagg attatttacg tcgttgacct gataacggcg cacatcagcg cccatcttaa
     1981 acgtatggcg gccgcggtac caggaaaggt tgtcaaccgc ctgatagtcg ttttgtactg
     2041 aagcatagga gttcacccag gtaccggtgc tttcaaaagg aatcgcgccc gagaaactga
     2101 aattaggcag ccctccaatg gccggatcgt tggcggggtt gtcgatcccc tcaatgccaa
     2161 gatccggctg cttaccgaaa ttgtagtcac tgccgaaata gccgtttcta ctatagccta
     2221 acttaccctc attcaccaaa gtcgggctga aggtgtgagt gtctgaaatt accacagttc
     2281 ggccaggaac attgccataa aagctactac ttccgtaccc gtccttcatg gcgcctttga
     2341 ttacgtcatg attatggatg gtcagaccga cacgaacaaa aagagtgttg ctattggtga
     2401 tcttgtgatc cactcgggtt gagagtccat cggcattgaa cttgtagccg ggatccgcat
     2461 agaagttctc ggtgatgcca aaatctccga gtccagtaag attagggtcc ggataaacga
     2521 gatcctggac cgccctggac acggagctga tccgatcccc cggtatttta ttgtcgttaa
     2581 aaggcacgcc cgtcaatggg tcgacgattg tgatctgacc aagaagggcc gagaaatctc
     2641 cctggcggaa agcgggaggt gggacagagg tccgcgtgcg gctccccgtg cgataacggg
     2701 cgccgctgta gtggaaaaag tagaaggtct tgtcgcgccc gtcatagatt ttgggcaggt
     2761 agacggggcc gccattgttt atcgcaaaca tgttgtggtt ttcgaacgac ggcttggtgg
     2821 gatcgttcca cgcccgggcg ttgaatctgg aattgaaatt gccccagtaa aaactgccat
     2881 ggaagccgtt ggagcctgac cggctgatca tcgacatctg ggccacctgg gaatattcgg
     2941 ccgagttgtt ggcggtcatc accttgaatt cctggtagct ttcaatatcg ccgcctggaa
     3001 agccattggc ggtctgccgg ctgctgacga catcgttaac gacgatgccg tcggaggtga
     3061 aattattggc ggtatcgcgg gccccgttga tgacaacgcc acaacagccg tcaacacctc
     3121 cgctctggac gccggcggtt acgaagacca cgttgggcca tccgcggcca taaacactct
     3181 gaggtagttg gacgaagtcc tgtgtgctct tgacattcga gagggccgcc gtttcggtct
     3241 cgagtagacc gacgtctccc gtgaccgtca cggtggttgc ggtctcgcca acttgcatgc
     3301 ttgcgtccac tcgtttgatc tgacttgact ccagaacgat ccggtttcgc acgaaagtgt
     3361 tgaacccggt cgaagtcacg cgaacttcgt agctcccccg ctccagcccg gcgacgcgat
     3421 aatctccgtt ttgatcggtc attatctgcc tcttaaagct ggtgccttcg ttggtgacct
     3481 ctaccgtggc cccgacgatg acccctccag atgggtcagt cacggtcccc agaatggtgc
     3541 tttgggtgga ctgtcccttc atgtcgaccg ttgcgagcag caagaggctg ggcacaagca
     3601 gcaggaagta acgctttgcg gagtacccca tcttccacct cccagtggaa tagtcacaat
     3661 ttacccggaa ccgtgacttt ctggcgacca gaagccggag attcgtttgc taactatata
     3721 atattagctc ggggttctgt gtcaagagaa taaagcgcgc cgggccagaa agtgagtgct
     3781 cgggctccgg actggattaa cacagcgccg gaattcggag ggtgccagct ctcaaactga
     3841 gaggaactac agtaaggcaa gagaaagtgc tctaatttcg gaaaacttcg tagtcaggaa
     3901 gaagtcgctc tctatcagcc tgttgatctg gtgcgtgtaa atttccacgc cgatcctcgc
     3961 aggccacggg aaacgatggc ctgcgctacg tacgccgacc ccaaaggtgt gcattgtctg
     4021 ttactgtatc cagaagtccg gaactggagg acgctatgga ttcgaaagcc ggttggaacc
     4081 ggagaaggtt gttgcaggcc gtgggcgcga gcgtgcccac gttgaaactc gtcggggttg
     4141 ccgcctccgc cgcacaaggg gacggcgcca agttcacacc catcgacctt agcccttact
     4201 tcaacgcgtc gtcggcggaa ttcggtcccc gcggaatggg ccgtcagacg ggagcacccg
     4261 gcgcgcgagg cgacgtcctg attcgtccgc cgggcggaaa gcaggagctt cagggcattc
     4321 ccttcctgct ggggccggag gacgttcaac agaagtcgtg gatcgcggtc agcacggccg
     4381 ggcagccatg ggctgctcca aagattgaga tcccaatcgg acataccgcc ccatgcctct
     4441 gcctggcgca gttctgcgac tgggatccta acgagatcga tcagagcagc ttcgagagca
     4501 tcgagcatct cgggcaaaag ctggccgagg ccacgctggt atacgaagac ggatctgagt
     4561 tttcggcacc catccggcgg cgccttgagg tccatgctcc gtcaatttct ttcggctacc
     4621 agtgttttct cgctatgccg agcagcactt gggaaccggc gaagctcacc gatccgcttc
     4681 ccaatggcct gggatggggg aacttgcaga cggtggccaa gggtaagagt cccgggcagg
     4741 cgccgttgtg gatctgggcg ctggaaaatc ccaatcccga ccgcaaggtg aaggcgctgc
     4801 gggtgcgggc agtggccgaa gatcccgtgg tgctctgcgg gctgacactc tttcacgggc
     4861 gcgagcaccc gttgcgcagg gagaggcgga cactgtaccg catcacgctg cccagcccga
     4921 cggcggaaga aaagggccgc tggacagtcg aggtagatct gggtgtcgtg gtgcgcacgt
     4981 acgtactgcc ggagttcgac cccgagtcgt ggctacgagc cgacggcgca ggattcggcg
     5041 agcggagcgc ggagccgagc cagtcacggc atctgtacgc cgaaatcgcg gcgagcgccg
     5101 atgcaacgct tgaagtccgc gatacagcga gtggcgggcg ttaccagttt gacctgatcc
     5161 gtgcagtgcc gggacaggag ctggaggcgc gccgcgggag ctcgcgcatc gaaatcctgg
     5221 agcgcgagaa aacgtggatt cacggccagg tggtggattc cgccacgggg cgtccgacgc
     5281 cggtgcgtct tgctttccgc tccaaggcgg gccggtacat tcctccctac ggacaccgga
     5341 cggaaatcaa cggcgggtgg ttccaggact acgccgccga cgccaagatc agctcgaatt
     5401 cctacgcgta tgtggatggg acgttccagg ccgagctgcc ggtgggagag ttgtacgtcg
     5461 aaatcagcaa gggattcgag tacaaggcga tccgcgagaa gctggaaatc cagccgtcgc
     5521 agcgtgagtt gaagctacag atcggcaagc acgcggacct gcgctccaaa ggatgggtga
     5581 ccgcggatac gcacgtgcat tttctctcgc cgtcaacggc ggtgctcgag gcgcaggccg
     5641 aagggctgaa cctcgtcaac ctgctcgccg cgcagtgggg cgatctttac accaacgtgg
     5701 gtgatctgcc gttcgggccg ctcacctcgc cggacaaaga gaccgtcgtg tgggtgggca
     5761 cggagaaccg ccagcacctg ttggggcata tcgggcttct cggcggccgc ggcgagcccg
     5821 tgttcccgat gtcggcggac aatccgtccg aggcttttat cggtgatcct ttgtggagca
     5881 gcatggccga gtgggcggac gcctgccgca aacgggaggg cgtagtggtg gcggtacatt
     5941 tcccgaaccc cttcgccgaa ctggcggctg atatcgtgct gggcaagatt gacgcggtcg
     6001 agttgtatcc tcagggcgac ggcggattcc gaactcgtgg gtacaacgac tggtaccggt
     6061 atttgaactg cggctatcgc gtgacagcgg cgggtggaac ggacaagatg ggcacgtaca
     6121 cgccggtggg cggcaaccgc acgtatgcat acatcggcca ggagcagttc gacttctcca
     6181 gttgggcaaa ggcagttcgc gccggccgca ccttcgcgag cagcggaccg ctgctgctgt
     6241 tccaggcaga cgggcgaatg ccgggcggcg agatcccgat gcggaagggt gaggccgcgg
     6301 tcgaggtgtc tgtggaagcg aacagccatg tacctttcca ccggattgag atcgtacaca
     6361 acggccgtgt cgtcgcctcg cgtgaagaga aggcggggac caagcggatg gcgttctcgg
     6421 aatccgtgaa agtgcaggcg cctgggtggc tgacggcgcg gtgttcgtcg acgctggggc
     6481 cttccctgtc ggcacggttc gggatcgcgg cgcacacatc gccggtttat ctgagtgttg
     6541 caggagaaga gcagttctcc gagccggctc tcacgtacct cttgacactt gtggagggca
     6601 ctcggggcta tgtggagaag ctggcaactc gccctgacac ggagagcttc gcacgagtgt
     6661 tgaaggtgtt cgcggacgcg cgtgagcacc tccacaggcg gctgcacgag cagggccgat
     6721 agggcgaagg gagcccaaag aagatattcc ggtcgtatct gctgcttttg ccttataatc
     6781 ggccccgggg aaaggcgcag acatgaacca ctgtctgaag ttgttcggac tcctcttcgt
     6841 cttcgcgacc ctggccgcga cagaccaacc ggtggttt
//
LOCUS       DATIFQ010000020         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2687764, whole genome shotgun sequence.
ACCESSION   DATIFQ010000020 DATIFQ010000000
VERSION     DATIFQ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687764"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VLE22_00765"
     CDS             complement(<1..379)
                     /locus_tag="VLE22_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12954.1"
                     /translation="MERHFPEDTFAKFTYVPVLRALAALGRGNPAESVERLQTALRYE
                     LAANGLNFAHHYLGGLHSAYVRGEAFTAARRWAEAAAEFQKILDHRGVVGLDPIGPLA
                     HLELGRVFALSGDKAKAKAAYEAF"
     gene            complement(408..1592)
                     /locus_tag="VLE22_00770"
     CDS             complement(408..1592)
                     /locus_tag="VLE22_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HSB12955.1"
                     /translation="MAHQPDSSVIETVVQLLCESGLSQMAEAVRIMLNEAMRIERCQV
                     LEAGPYERNERRRGYANGFKPKTLETRLGAMTVQVPQTRGVEFYPSALEKGVRSERAL
                     KLAVAEMYVQGVSTRKVTDVMQQLCGLEVSSTQVSRAAQLLDEELTAWRQRPLGEIPY
                     LVLDARYEKVRHGGSVVSCAVLIAVGINPEGHRTLLGVSVSLSEAEVHWREFLAGLQD
                     RGLHGVKLIVSDDHAGLKAAREGRLPGVPWQRCQFHLQQNAGHYVPRVSMRSEVAADL
                     RAIFDAPDRVEADRQLELAMRKYEKTAPKLAAWLATNVPDGLTVFALPPSHRRRLRTS
                     NLLERLNKEIKRRTRVATLFPNEAALLRLVSAVLMEISEEWETEKIYLRMENSGSIAR
                     EE"
     gene            complement(1735..>2034)
                     /locus_tag="VLE22_00775"
     CDS             complement(1735..>2034)
                     /locus_tag="VLE22_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12956.1"
                     /translation="MVRQANTTDQQLSLMRMQFESADRPWLVIDASVATPLTIDDAAA
                     HVGFDFLVTNTGRSPAQNIWIRPELVPSVLLMNSTAKQRRNCWCNILSVNSVQSN"
BASE COUNT          390 a          648 c          583 g          413 t
ORIGIN      
        1 gaaaagcctc ataggcagct tttgccttgg ccttgtcccc cgatagcgcg aacactctac
       61 cgagttccag gtgcgccagc gggcctatgg gatccagccc tacgacaccg cgatgatcga
      121 gaatcttctg aaactcggca gccgcttccg cccagcggcg cgcagcagtg aacgcctcgc
      181 cgcgcacata ggccgagtgc aaaccgccga gataatgatg agcgaagttc aggccattcg
      241 ccgccagttc atatcgaaga gcggtttgca gccgctcgac gctctctgcg ggattgcccc
      301 gccccagtgc ggctaacgca cgaagaaccg gtacgtaagt aaatttggca aacgtatctt
      361 ccgggaagtg tctttccaaa tgacaaagtc aaggcctttc tgtaaattca ttcttcccgc
      421 gcgatcgagc cactgttttc catccgtaag tagatctttt ccgtttccca ctcctcactg
      481 atttccatca ggacggcgct gaccagccgc aagagggcgg cctcgttcgg gaacagggtc
      541 gccaccctgg tccggcgctt gatctctttg ttgaggcgct caagcaggtt cgaggtgcgg
      601 aggcggcgcc ggtgcgacgg cggcagcgcg aaaacggtca gcccatccgg cacgttggtt
      661 gccagccaag cggcgagctt gggcgcagtt ttctcgtact tgcgcatggc gagttccagt
      721 tggcgatcgg cctcaacccg gtcgggcgcg tcgaaaatcg cccggaggtc tgcggccacc
      781 tcgctgcgca tcgagacgcg cgggacgtag tggccggcat tctgctggag gtgaaactga
      841 cagcgctgcc agggcacgcc gggaaggcgg ccttcgcgcg ccgccttcag gccggcgtgg
      901 tcgtcgctga cgatcagctt gacgccgtgc agcccgcggt cctggagacc agcgagaaac
      961 tcgcgccaat gtacttcggc ctcactgagc gaaacgctca cgcccagcag cgtccggtgt
     1021 ccttccggat tgatgccaac cgcgatcagg accgcgcagg agaccaccga tcccccatgt
     1081 cttaccttct cgtatcgggc atccagcacc aggtacggga tctcgccgag cgggcgttgt
     1141 cgccatgcgg tcagctcctc atccaggagc tgggcggcgc ggcttacctg cgtgctcgac
     1201 acttccagcc cgcagagctg ctgcatgacg tcggtcacct tgcgcgtgga aacgccctgg
     1261 acgtacatct ccgcaacggc cagcttcagg gcccgctcgc tccggacgcc cttctccagt
     1321 gccgaggggt agaactccac cccgcgggtc tggggtacct ggacggtcat ggctcccaat
     1381 cgggtttcca gcgtcttggg cttgaaaccg ttggcatagc cccgccggcg ctcgtttcgt
     1441 tcatagggac cggcctcgag cacctggcat cgttcgatcc gcatcgcctc gttgagcatg
     1501 atccgcacgg cctccgccat ctgcgacagg ccggattcac aaagcagttg aaccaccgtt
     1561 tcgattacgc tagagtctgg ttggtgggcc atttcagtct cctttctctg gaattcgcct
     1621 tccacaaaag gaaaacagat ttggcccgcc cagcacaagt ccgcccgatt ttgttttgac
     1681 cctccgctcc ggctcggttc cctcgcctcc actccgggcc aaaacaaaat cgcttcaatt
     1741 gctctgaacg gaatttacag aaaggatgtt acaccaacaa ttccgccgtt gtttcgctgt
     1801 cgaattcatg agcaacaccg agggaaccaa ctcgggtctg atccatatgt tctgcgctgg
     1861 ggatcggccg gtgttggtga cgagaaaatc aaaccctaca tgtgcggccg catcgtcaat
     1921 ggtcaatggg gtagcaactg aggcgtcgat cacaagccat ggacgatcag cagactcaaa
     1981 ctgcattctc atcagggaaa gctgctggtc tgtcgtgttc gcctgacgga ccat
//
LOCUS       DATIFQ010000021         3779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_772341, whole genome shotgun sequence.
ACCESSION   DATIFQ010000021 DATIFQ010000000
VERSION     DATIFQ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772341"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VLE22_00780"
     CDS             complement(<1..1323)
                     /locus_tag="VLE22_00780"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HSB12957.1"
                     /translation="MKRTAEAVRPDLPRISTPLPGPNAREVIERDRRVISPSYTRSYP
                     MVAARGEGAIVEDVDGNRFLDFNAGIAVVATGHCHPKVVESIRAQAGRLIHMSGTDFY
                     YENMVELAERLAALAPGSEPRRVYFGNSGTEAIEAALKVARYHTGRDKFIAFLGGFHG
                     RTLGSLSLTASKTVQRRGFGPLVPGVHHVPYASCYRCAYGKQPDTCAVECVRAIEDQL
                     LKTVLPAEETAAIVLEPVQGEGGYVVPPQKFFDELQALARRHGILIVADEVQSGMGRT
                     GRMWASEHFGLIPDVMAVAKGIASGMPLGATIARAELMQWKPGAHASTFGGNPVAVAA
                     ALTTIELLEQGLVDNAARMGAFLMDRLHDWPRRFPWVGDVRGLGLMIGIEFVRDQATR
                     ERAPEFRDRVVRMAFERGLLVLGAGQNTVRLSPPLTISRDQAAFAADTL"
     gene            1487..1918
                     /locus_tag="VLE22_00785"
     CDS             1487..1918
                     /locus_tag="VLE22_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HSB12958.1"
                     /translation="MQGLRFVCQAECTKCCEVSGYVYLTEQDLKRAAAHLGMSAPSFE
                     KKYIYRTKHLLRLRKPRGSQCHFLEKGGCSIHPVKPLQCRVFPFWPEKIGSPTAWRET
                     GRMCPGIGEGPLIPIDGVMRVANEMREAYMGMYSRPGRVTG"
     gene            1944..2807
                     /locus_tag="VLE22_00790"
     CDS             1944..2807
                     /locus_tag="VLE22_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12959.1"
                     /translation="MIREALRLAGFQALKPLERRLLEELSARLGPGAQKLLAAQIGRI
                     NLVQRHGKATEVCLYRVLFGSVRHDPRYRFPNQSLECRLATIRFRVPGMNPVWVADIS
                     VVRGFLFSIDFRPPAKPILNRSDIEVEHVKIHESPMEEKPARYPEGPRDLDAATFPPN
                     GWFAPWRDRYRLSNVTPPLAAAERERRLAEIDASLPEDFLFLVSQTDGVTVEGQCSRA
                     SILGLAATYAVSLENGRHYLLAQLGDQGYLGTPAGNGGGRVVFLGFDGSFRFVDGFRP
                     AVQRFVETGVV"
BASE COUNT          735 a         1093 c         1214 g          737 t
ORIGIN      
        1 tagtgtatcc gcagcgaatg ccgcctggtc gcggctgatg gtgagcggcg ggctgagtcg
       61 caccgtgttc tggccggctc cgagcacgag cagcccgcgc tcgaaggcca tccgaacgac
      121 gcggtcgcga aactcgggag cacgctcccg tgtggcctgg tcgcggacga actcaatgcc
      181 tatcatcaac ccgaggcccc ggacgtcgcc gacccaaggg aagcgccgcg gccagtcgtg
      241 gaggcggtcc atgagaaatg cacccatccg ggctgcgttg tcgaccaacc cctgctcgag
      301 cagttcgatc gttgtcaggg cggctgccac agcgacgggg ttgcctccga atgtcgaggc
      361 gtgcgccccg ggcttccatt gcatcagttc cgcgcgcgcg atcgtggcgc cgagcggcat
      421 tccgctcgcg atacccttcg ccaccgccat gacgtctggg atcaggccga agtgttccga
      481 cgcccacatg cggccggtgc gtcccatgcc ggactgcact tcatccgcga cgatgaggat
      541 gccgtgccgg cgggcgagcg cctgtaactc gtcgaagaac ttctgcggcg gcaccacgta
      601 ccccccttct ccctgcacgg gttcgagtac gattgcggcc gtttcctcag ccgggaggac
      661 cgttttcaga agctggtctt cgatagccct gacgcactcg acggcgcagg tgtcgggctg
      721 tttcccgtac gcgcagcggt agcaagaggc gtacggaacg tggtggacgc cgggaacgag
      781 cggtccgaat ccgcgccgct gcaccgtctt gctcgccgtg agcgaaagcg agcccagtgt
      841 gcggccgtgg aacccgccga gaaaggcgat gaatttgtcg cggccggtgt gatagcgcgc
      901 gactttcagc gctgcttcga tcgcctcggt gcccgagttg ccgaagtaga cccgccgggg
      961 ctcgctgcca ggggcgaggg cggccaggcg ttcggcaagc tccaccatgt tttcgtagta
     1021 gaagtcggtt ccggacatgt ggatcagccg cccagcctgc gcccggatcg actcaacgac
     1081 cttcgggtgg caatggcctg tggcgacgac cgcgatgccg gcgttgaaat ccagaaaccg
     1141 gttcccgtcc acgtcctcga cgattgcccc ctcgccccgc gcggcgacca tcggatagga
     1201 ccgcgtgtac gacggcgaga tcacacgccg gtcgcgctcg atcacctcac gggcgttggg
     1261 gccgggcagt ggagtcgaaa tgcggggaag atcgggacgg accgcctcgg cagttctctt
     1321 catcttccta acctcctgag tactgggaaa ttcctgaaaa gaaagagatg ggcgggcagt
     1381 atcagtcgca gccgaacagg gcgggccggg cgatggctgg ccggaccttc ataccttcat
     1441 tctacgcctc cctccggagg ttgggaagtg ggctatcctt atacgcatgc aggggttgcg
     1501 gttcgtctgc caggcggagt gcacaaagtg ctgtgaggtc tcgggctatg tgtatctcac
     1561 cgagcaggat ctcaagcggg cggcagcgca tctggggatg agcgcgccat cgttcgagaa
     1621 gaaatacatc taccgtacga agcacctctt gcgcctgcga aaaccgcgcg gatcgcagtg
     1681 ccattttctc gagaagggcg gctgctcgat ccatccggtg aaacccctgc aatgccgggt
     1741 gttcccgttc tggccggaga aaatcgggtc gcccacagcc tggcgcgaaa ccggccgcat
     1801 gtgtccgggg atcggcgaag gccccttgat cccgattgat ggagtcatgc gcgtcgccaa
     1861 cgagatgaga gaggcctata tggggatgta cagccggccg ggccgtgtga ccggctaact
     1921 gacccctccc ggagaagggc attgtgatca gggaagcgct gagattagcc ggcttccagg
     1981 ctctcaagcc actggaacgc cgcctgctgg aggagctcag cgcgcggctt ggcccgggag
     2041 cccagaaatt actggccgca caaatcggga gaattaacct cgttcaaaga cacggcaagg
     2101 cgacggaagt ctgtctctat cgggtgctct tcgggtcggt tcgacacgat cctcgttacc
     2161 ggtttcccaa ccagtcgctt gagtgccggc ttgcgactat ccggttccgc gttccgggca
     2221 tgaatcccgt ttgggtcgct gacatctccg tggttcgcgg atttctgttc tcgatcgatt
     2281 tccgaccgcc cgcgaagccg atattgaacc gttcggacat cgaagttgag catgtgaaga
     2341 tacacgagtc tcccatggag gagaagccgg cccgatatcc ggaagggcca cgggacctgg
     2401 acgccgcaac cttccctccg aacggctggt tcgcgccctg gcgagatcga taccgtctca
     2461 gcaatgtgac gcctcctctg gcggctgctg agagggaacg gcggttggca gaaattgatg
     2521 caagtcttcc agaggacttc ctctttcttg ttagtcagac agacggagtg actgtcgagg
     2581 gacagtgttc gcgggcttcg attcttggac tggcagcaac ctacgcggtt tcactggaga
     2641 acggcaggca ttatcttctt gcgcagttgg gggatcaggg ctatttggga acgcctgcag
     2701 gaaacggagg aggacgggtc gtgtttctcg gcttcgacgg ttcgtttcgc ttcgtcgacg
     2761 gttttcgccc ggcagttcaa cgcttcgtcg aaacaggagt ggtatgagtt ggcacgtgtc
     2821 caagagaagc ggtcgtcaag ccaatgacta ctgccagccg ggcgggtctg aatgccgcca
     2881 gggcctgtag ctgcaaatga agataacgtg gctcgatttt caggcctacg gcactattct
     2941 ggtcgcctcg catcttgtcg cttacctaac tgcaccacta tggggtatgg catattgggt
     3001 cgaatttgcc gccgtcagcg ttatcctgtg tttggtctgg aggaaatatg aggacaggtc
     3061 cagacgtcaa tggagaagaa acgaggatcc gctaagaacg agaaccgaga gcatgagcat
     3121 ccaggaagca cgcggggccg ctcacgaagt tctccgggac ccggcgaagt ttcgaacggt
     3181 cccagttgtg gacacttggc ttccccgagg tgaacagtta ccgtcagccg tgcgagagtt
     3241 gttcggaacg tattcctcaa tcgaagttgt cgctggtgaa gcccgtttgt cacaagacct
     3301 catctccgcg tctgaatttc acccggggtg gatacggatc ggaacggata tggactttac
     3361 cgagatcgca gtccgcccag gtgacgaagg cgtatatgag tttgacggtt cggagtccac
     3421 gcagaacgac tttgatcaag cttgttcatc cagtgtctac cattggattg tcatgacggc
     3481 tgagaactta tacggggcat gaaggacggg tggcggaccg gcaagaccag ggaaacgggg
     3541 gggcgcctac agcgggccaa gcggtgggac gcagcgcccg tccgatttga cagcgagcgc
     3601 ccaagtcctg tagcctaaga attttacggc agtaatcgaa gaggaaacgt ctgtccatga
     3661 acaccgtatt tccgtccggg aacgagatca agcgcaactg gttcgtgctt gacgccggcg
     3721 accaggtact gggtcgagtg gcaagcaaag cggccatgat tctgatgggc aagcacaag
//
LOCUS       DATIFQ010000022         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1921963, whole genome shotgun sequence.
ACCESSION   DATIFQ010000022 DATIFQ010000000
VERSION     DATIFQ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921963"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VLE22_00795"
     CDS             complement(<1..478)
                     /locus_tag="VLE22_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12960.1"
                     /translation="MPSALERVRQAAIRNKGERFSALLHHVTADRLRDAFYHLRRNAT
                     PGVDGVRWEHYEADLDRNIEDLHARVQRGAYRAKPSRRAYIPKPDGRQRPLGIAALED
                     KVVQRAVTEVLNAIYEADFLGFSYGFRPGRRAHVALDALAVGIRFRKISWILDADIR"
     gene            complement(727..1560)
                     /gene="istB"
                     /locus_tag="VLE22_00800"
     CDS             complement(727..1560)
                     /gene="istB"
                     /locus_tag="VLE22_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HSB12961.1"
                     /translation="MKTTTRKNRLTTTPTDLAAASGSLRDRCLRHFAVLRIPVEAAML
                     DEVLTRAEKETLSHLAFLDRLLGAEADARRERAVSRRIHQARFADDKSLEGFNWKFNP
                     KTFDRLQIEELATSDFIRRRTNLVMVGWSGIGKSHIIQALGQKACVNGYRVLYRTSAQ
                     LLTDLTASLADKTLPARLRYYANPDLLIIDEFGFDKIERAECPEAAHLLYKIIAARNQ
                     KRSTALVTNVDFEKWGEYLSDGPLAMAFLDRLVEGAIILKLKGKSYRADPALTPEIPA
                     S"
     gene            complement(1514..>2679)
                     /gene="istA"
                     /locus_tag="VLE22_00805"
     CDS             complement(1514..>2679)
                     /gene="istA"
                     /locus_tag="VLE22_00805"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HSB12962.1"
                     /translation="STYEIPFTAEGRRRVQAFSFILGYSRRQYIRFVENQDFTTTIRE
                     HVRAFQLFQGLPATCLYDNMKVVVSSYDGDQPVYNTRFLSFATHYGFQPWACRPHRPQ
                     TKGKIERPFAYIETNLLNGRTFTSLDHLNEIAALWLTETADVRIHRETKRRPIDLFEE
                     EKPHLIALPARPYDTARVLYRTVNPEGFIPYLQNFYSVPWQRIGELLPVRVTEDELIV
                     YGADVKEIARHELFPAGVTGQNRHRPEHASARDSQQKRELLKQRFEEFGTDGILFFDG
                     LLKSRRNGKDEAGRVLTLLAIYRREDLGRALERAVRYRAYSYTAVERILAAQAKPRSA
                     REALVIEARDHLHDILRETRLGSRSTADYQALLEETADHENDNPEEPADYDPD"
BASE COUNT          479 a          806 c          842 g          552 t
ORIGIN      
        1 ctcgaatatc cgcatccagg atccagctga tcttcctaaa acgaatcccc acggcaagcg
       61 cgtcgagcgc cacatgggct cggcgtcccg gtcggaatcc gtatgagaag ccgaggaaat
      121 ccgcctcgta gatggcgttg agcacttccg tcacggcccg ttggactacc ttgtcctcaa
      181 gggccgcgat tcccagcggc cgttgccggc catcgggctt cggaatgtat gctcggcgag
      241 aaggtttcgc ccggtacgct ccgcgttgca ctcgcgcatg caggtcctcg atatttcgat
      301 ccaggtctgc ttcgtagtgc tcccacctga cgccgtcgac gccgggcgtc gcgttcctcc
      361 tgaggtgata gaatgcatcc ctcagacggt ccgccgtcac gtggtgaagg agcgcgctga
      421 atcgctctcc cttattcctg atcgctgctt gtcgcacgcg ctccagcgca ctgggcacgc
      481 atacttcccg gctctgtgtc cggcgcgtgt tttgctgaag cacgttctcc ctggtcagcc
      541 cccttccctc cgccacctcc gctgcgagtg tccccgcttt gttcgatggc ttcgtcggta
      601 ttatggagct gtctgtcggt ggcgccgaag agttgggcca aaatggcagt caggaagggg
      661 ccagcagcgc agttacgcta tggccgattc cggcgcgtgc gaaaagcacg acagtgggcg
      721 gcggtgtcag gaggccggaa tctccggcgt aagtgcgggg tcggcgcgat aggattttcc
      781 cttgagcttg aggatgatgg cgccctcgac caggcgatcg aggaacgcca tggctagcgg
      841 accatcggac agatactcgc cccacttctc gaagtccacg ttggtcacca acgctgtaga
      901 ccgcttctgg ttccgcgcgg cgatgatctt gtagagcagg tgcgccgcct ccggacactc
      961 ggcgcgctcg atcttatcga acccgaactc atcgatgatg agcaagtccg gattcgcgta
     1021 gtagcgcagc cgagccggaa gcgttttgtc agcgagcgac gcggtgagat cggtcagcaa
     1081 ctgggccgag gtccggtaca gaactcgata gccgttgaca cacgccttct gccccagtgc
     1141 ctgaatgatg tggctcttgc caataccact ccagccgacc ataaccaggt tggtccggcg
     1201 gcgaatgaag tcgctggtgg ccagttcttc gatctgcagg cggtcgaagg tcttggggtt
     1261 gaacttccag ttgaagcctt ccaggctctt gtcgtcggca aagcgcgcct gatgaatgcg
     1321 ccgcgacacg gcgcgctcgc gccgggcgtc ggcttctgcg cccaacagac ggtcgagaaa
     1381 ggccagatgc gacaaggtct ccttttcggc gcgcgtcaga acttcatcca acatcgcggc
     1441 ttcgaccgga atgcgcagca ccgcgaagtg tctcaggcag cggtcgcgta aagagccgga
     1501 tgcagcggcc agatcagtcg gggtcgtagt cagccggttc ttccgggttg tcgttttcat
     1561 ggtccgccgt ttcctcgagc agcgcctggt agtcggccgt ggagcgcgag cccagccggg
     1621 tttcgcgcag aatgtcgtgg agatggtcgc gcgcttcgat gaccagcgcc tccctggccg
     1681 accgcggttt ggcctgcgcg gccagaatcc tctccacggc agtataggaa taggcgcggt
     1741 aacggaccgc ccgctccagc gcccgtccca gatcctcgcg gcggtaaata gcaagaagcg
     1801 tgagcacgcg ccccgcttcg tcctttccgt tacgccggga tttgagcagt ccgtcgaaga
     1861 acaggattcc gtccgttccg aactcctcga agcgctgctt caacagttcg cgcttctgct
     1921 gactgtcgcg cgcggacgca tgttccggcc gatggcgatt ctggcccgtc actccggccg
     1981 gaaacaactc gtgacgcgcg atctctttga cgtccgcgcc gtagacgatg agttcgtctt
     2041 cggtcaccct caccggcagc agttctccga tgcgttgcca cggcacggaa tagaagttct
     2101 gcaagtaggg gatgaagcct tccggattca ccgtccggta caacacacgc gcggtgtcgt
     2161 agggccgggc gggcaaagcg atcagatgcg gtttctcttc ctcgaacaag tcgatcggac
     2221 ggcgcttggt ttcgcgatga atgcgtacat cggcggtctc ggtgagccag agggcggcga
     2281 tctcgttgag atgatcgagc gaggtgaagg tccgtccgtt cagcaggttg gtttcgatat
     2341 aggcgaaggg tctctcaatt ttccccttcg tttgcgggcg atgcggcctg cacgcccagg
     2401 gctgaaagcc gtagtgggtg gcgaaggaca gaaagcgcgt gttgtagacg ggttggtcgc
     2461 cgtcgtagct cgagaccacg actttcatgt tgtcgtagag acaggtcgcg ggtaatccct
     2521 gaaacagctg gaaggcccgg acatgctcgc ggatggtggt ggtgaagtcc tggttctcga
     2581 cgaagcgaat atattgccgc cgcgaataac caaggatgaa gctgaaggcc tgcacacgcc
     2641 tgcgcccttc ggccgtgaac gggatttcat aggtcgaat
//
LOCUS       DATIFQ010000023         8678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2113464, whole genome shotgun sequence.
ACCESSION   DATIFQ010000023 DATIFQ010000000
VERSION     DATIFQ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113464"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VLE22_00810"
     CDS             <1..1264
                     /locus_tag="VLE22_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB12963.1"
                     /translation="QNGPMPPIVAVASVWRDGYFESAMTGKERIALTMRHQEPDRVPV
                     MCQLSIGHYNLNGGYKPHEIWYETEAFADASVKLARRYAFDGILVVLTGRPPNYLDES
                     VSIKAENDGEWITWRNGDRTFMPWDDMPHHYPADTSKPARADFETFDPDRDLDRVDEY
                     LGYTWNVLFHIQDVPGKPDAGLLRPGEMPDYLFRAFDLVKERAGDDLSIHGSIYSPLS
                     HYFELFGYEEALTAFVTDPGKVHAILERLAGNTIAWGRALAARGADALDLSSAFVAAP
                     FLSRGMYREFVVPYERRVNEALREAGCIVYTHTCGRIGDRIDLLAETGTCGIDTLDPP
                     PLGNCEIAEAKRDFGDRLFFKGNMNSVALLQYTTQEEVRAEATRPLRIGKPGAGYILS
                     TACSVAPRVEPWKLEMLAPLAEEIGRYS"
     gene            1338..3437
                     /gene="glgX"
                     /locus_tag="VLE22_00815"
     CDS             1338..3437
                     /gene="glgX"
                     /locus_tag="VLE22_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HSB12964.1"
                     /translation="MSTSVAGVSGVTYVAEPGRTHPLGAIPDREGVNFSVFSANATGV
                     ELLLFESHDSPFPFQVIALDPYVNRTFHFWHVFVRGLKPGAHYAYRVDGPWNPAAGHR
                     FNRKKVLIDPYSRGNTDALWDRAAACGADDNVASSMRSVVIDTAGYDWDRDQPLDQPV
                     NDLIIYEMHAAGFTKSPTSRVRHPGTFSGIIEKIPYLKGLGITAVELLPIFDFDEAAV
                     LRVHNGEPLRNYWGYSTISFFAPQCAYCVDPAKGAHLTEFRDMVKALHKAGIAVILDV
                     VFNHTDEGNHEGPVLSFKGFENSVYYFLVPWNREFYFDFTGCGNTMKCNHPIVQKLIV
                     ECLRHWVQETHVDGFRFDLGSVLARGEDGAPLANPPVVWQIELDEYLADTKVIAEAWD
                     AAGLYQIGYFPGNRWMEWNGRYRDDIRRFVKGEPGLVGAVASRIAGSADIYQPAGELP
                     VNSINFITSHDGFTLNDLVSYNEKHNEANGEHNRDGANDNDSWNCGVEGETDDPAVEA
                     LRARQIRNFAAILMLSRGVPMFTAGDEIRRTQKGNNNAYCQENEISWFDWDLVEKNWD
                     MFRFWSLMIDFRKRHSTLHDPHFFRGRVNGRGVADVTWHGCKLNQPGWNDPAAKALAF
                     TLGGFGGDPDLHVMMNMHWERLEFEVPAVPGRRWSRAINTALPPPDDIAEAGIEAAFD
                     GSACVVEGRSIVVLVSA"
     gene            3504..4937
                     /locus_tag="VLE22_00820"
     CDS             3504..4937
                     /locus_tag="VLE22_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HSB12965.1"
                     /translation="MRVILSFVLGAGLALAAPALRITGGAVDDQVFQRGPGNTVDIAL
                     SGTAADADSEPIEVRITRKFIPVEGWDWKVSGSVRQDQWSAELRGVPAGGPYRIEVRV
                     HGTTVSDSVRNVLVGDLWVLAGQSNMQGVGDLVDVEPPHELVHNFDMADHWVVAEEPL
                     HRLVDAVDRVHWSRNPQTKEPERFEGERLRKYIAERRKGAGLGLPFAVEMVKQMGVPI
                     GLVSCAHGGTSMDQWNPALKDQGGDSLYGSMVRRVREVGGKVAGVLWYQGESDANLKA
                     APEFQAKFERFIAAVREDFGQPNLPFYYVQIGRHVADANMAEWNLVQEGQRNAEESAA
                     RSGMAPAVDLSLDDSIHVGTQDLKRLGRRLANLALGRTRRGPRPLSAVFGDGMVRVVF
                     SDVNGRLTSKARIAGFSIHDASGASVPLIYKAQVDPVDGSAVLLFIGGKLPERATLRY
                     GAGRDPYCNLNDEADMASPVFGPMAIQ"
     gene            5015..5974
                     /locus_tag="VLE22_00825"
     CDS             5015..5974
                     /locus_tag="VLE22_00825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB12966.1"
                     /translation="MKLLLCAGLLLPLCSYAQTEGTAKPVAPAPAATLSTLPPSTVVA
                     TVDGTKVTASDLQAILRALPPASQQQALRSPRQFLDQFGLLRKLSAMAEQAKLHERSP
                     LREQLEYNRMVGMAQAQLLEAQNQIHISTEDAQKQYEANKAKYEQAKVKVIYIPFSAN
                     APAAQQSNAEKKVLSEAEAKEKAEKLLAEIKGGADFVKLVKEHSGDPVSKGKDGDFGN
                     IKRSDRIPEAIKTAVFALKPGEVSEPVRQPNGFYLFRLEESSQQMFDQVKDQITAEMR
                     QARFSEWMQSMQKSIDIKIENEAAFAPVAPAIQAAPLTPPVKQ"
     gene            6068..6592
                     /locus_tag="VLE22_00830"
     CDS             6068..6592
                     /locus_tag="VLE22_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12967.1"
                     /translation="MACLPAFEGKQVRVVLRPALFAHRGTLLSGLDLGAPVHAGSFLR
                     RREIVLDRSLCDEPSELARILVHEIFHFVWLRMGNHVRRSFEAKLAAELQRNARGELG
                     WSADLRKQTLSSGDVRGRNRRWREYVCESFCDSAAWLHTGAKGHDEFTLAPRFRRARR
                     EWMRHFEAAGRIPI"
     gene            6696..7220
                     /locus_tag="VLE22_00835"
     CDS             6696..7220
                     /locus_tag="VLE22_00835"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB12968.1"
                     /translation="MAKTSATSGKATTEDRKPAPEFSLKDGDGRTVSLAGYRGKVVLL
                     NFWATWCGPCKFEIPWFKEFEQNYKDRGFAVLGVSMDEEGWDVVKPFVAELQVNYRIL
                     MGDDTTAQLYGGVNSLPTSFLIDREGRIASVHVGLVGKGDYEDEIERLLAAPASGSFR
                     RAGVGMPAFALGAK"
     gene            7273..7620
                     /locus_tag="VLE22_00840"
     CDS             7273..7620
                     /locus_tag="VLE22_00840"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HSB12969.1"
                     /translation="MFATKVTVQLRNGYHVNSNTPADEYLIPLRLTWNASPLQVVDVA
                     YPKPRLENYSFSTKPVSVFTGDFDIVSRFKAPPNSPQGPSVLVGKLRYQACTATACLA
                     PKTVEVRLPVHIQ"
     gene            7630..8499
                     /locus_tag="VLE22_00845"
     CDS             7630..8499
                     /locus_tag="VLE22_00845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSB12970.1"
                     /translation="MRRLIIRPGAIGDCILSLPAMERLRAGYTEVWVAAQNCPFIRFA
                     DAVDSIGATSLDLVGLPGVEPPPGAITRLRGFDDIVSWYGSNRVEFQAAVAELGLPFR
                     FLTALPDETRGVHAADFYLEQAQSLTGCETGEIACPTLRIESLPRNEDFVVIHPFSGS
                     SRKNWPLKRYRELAERLAVQLSVRWCAGPEESLEGAARFDDLYELASWISRAAVYIGN
                     DSGISHMAAAAGAPVVALFGPTDPAVWAPRGPKVRIVSTRPAGMEMETIEVDEVLLAA
                     RELLSAQTRQRSE"
     gene            complement(8468..>8678)
                     /locus_tag="VLE22_00850"
     CDS             complement(8468..>8678)
                     /locus_tag="VLE22_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12971.1"
                     /translation="PARRITVTVDGVQVADKTFAAPGTYTLTSEPVVTLSDSPTVTIT
                     ADRTFSVAGDHRKLGVILSAVGFER"
BASE COUNT         1689 a         2464 c         2906 g         1619 t
ORIGIN      
        1 gcagaacgga cccatgcccc ctattgttgc agttgcgtcg gtctggcggg atgggtattt
       61 tgagagcgcg atgaccggaa aggaacgaat cgcgctgacg atgcggcacc aggaaccaga
      121 ccgtgtgccg gtgatgtgcc agctttcgat cggccattac aatctgaacg gcggctacaa
      181 gccgcacgaa atctggtacg agaccgaggc gtttgccgac gccagcgtga agctggcgcg
      241 gcggtacgcg ttcgacggca tcctggtggt gctgacgggg cggccgccga attatctcga
      301 cgagtctgtc tcgatcaagg ctgaaaacga tggtgaatgg atcacgtggc gtaacgggga
      361 tcgcacgttc atgccctggg atgacatgcc gcaccactat ccggcggata cgtcgaagcc
      421 ggcgcgtgca gacttcgaaa cctttgatcc ggatcgcgac ctcgacaggg tggacgaata
      481 cctggggtac acgtggaacg ttctgtttca cattcaggac gtgcctggca aaccggacgc
      541 cggactgctg cggcccggag agatgccgga ctatctgttt cgcgcgttcg acctggtgaa
      601 ggagcgggcg ggagacgatc tgtccattca cgggtcgatc tattcgccgc tcagccacta
      661 tttcgaactg tttggctatg aagaggcgct cacggccttc gttaccgatc ccgggaaggt
      721 gcacgcgatt ctcgagcgct tggcggggaa cacgatcgct tggggccgcg cgttggcggc
      781 gcgtggcgcc gatgcgctgg acctgtcgtc ggcgttcgtc gctgcaccgt ttctcagccg
      841 cggcatgtat cgcgaatttg tggtgccgta cgaacgccga gtaaacgaag ctctgcgcga
      901 ggccgggtgc atcgtctaca cgcacacctg cgggcgaatc ggggaccgga tcgacctgct
      961 ggcggagacg ggaacgtgcg gcattgacac gctcgacccg ccgccgctgg gcaactgcga
     1021 gattgcggag gcgaagcgcg atttcggcga ccgcctcttc ttcaagggca acatgaactc
     1081 agtggccctg ctgcaataca caacgcagga agaggtgcga gcggaggcga cgcggccgct
     1141 gcggatcggc aaaccgggcg cgggttacat cctcagtacc gcctgctcgg ttgcgccgcg
     1201 tgtcgaaccg tggaagctgg aaatgctcgc gccactcgcc gaggagattg gacgctactc
     1261 atagaagcgc cttgccggag cggcgcgcgg agcgtacgct ggctctattt gaggaattgc
     1321 cagaggagga accattcgtg tccaccagcg tagcaggggt ttcgggggtg acttatgtgg
     1381 ccgagccagg ccgcacgcat cctttaggcg ctattccaga ccgcgagggt gttaacttct
     1441 ccgtcttctc ggcgaatgcc acaggcgtgg aactactatt gttcgagtcg catgactcgc
     1501 cgtttccgtt ccaggtgatc gctctcgacc cgtacgtcaa caggaccttt cacttctggc
     1561 acgtatttgt gcgcggcctg aagccggggg cgcactacgc gtaccgggtc gacggcccgt
     1621 ggaatcccgc ggccgggcac cggttcaacc ggaagaaagt actcatcgat ccgtattcgc
     1681 gggggaacac ggacgctctg tgggatcggg ccgcggcatg cggagcggac gacaacgtgg
     1741 cgagctcgat gcggagcgtt gtcatcgaca cggccggtta cgactgggac cgcgaccagc
     1801 cgttggatca gccggtgaac gatctgatca tttacgaaat gcacgccgcg ggctttacca
     1861 agtccccgac atcgcgcgta agacatcccg ggacgttctc cggaattatc gagaagattc
     1921 cgtacctgaa agggctgggg attaccgccg tcgagctgct gccgattttc gacttcgacg
     1981 aggcggccgt gcttcgcgtg cataacggcg agccgctccg gaattactgg ggatacagca
     2041 caatcagttt cttcgcgccg cagtgcgcat attgcgtgga cccggcaaaa ggggcgcacc
     2101 tcacagagtt ccgcgacatg gtgaaggcgc tgcacaaagc ggggatcgcc gtgattctgg
     2161 acgtggtctt caaccacacg gacgagggca atcacgaagg gccggttctg tcattcaagg
     2221 gattcgagaa cagcgtctac tacttcctcg tgccgtggaa ccgggagttc tacttcgact
     2281 tcacaggctg cgggaacacg atgaagtgca accatccgat cgtgcagaag ctgattgtgg
     2341 agtgcctgcg gcattgggtt caggaaacac acgtcgacgg attccgcttc gatctgggat
     2401 ccgtactggc tcgcggtgag gacggcgcgc cactggccaa cccgccggtg gtgtggcaga
     2461 tcgagctgga cgagtacctg gccgatacca aggtgatcgc agaggcgtgg gatgctgccg
     2521 gactgtacca gatcggatac tttccgggca accggtggat ggagtggaac gggaggtatc
     2581 gggacgacat ccggcggttc gtgaagggcg aaccaggtct ggttggcgcg gtggcttcgc
     2641 gcattgcagg cagcgcggac atttaccagc cggccggcga actgccggtg aacagtatca
     2701 atttcatcac gagccacgat ggattcacgc tgaatgacct agtctcctac aacgagaagc
     2761 acaacgaagc gaacggcgaa cacaatcgcg acggcgcgaa cgacaacgat agctggaact
     2821 gcggggtgga aggcgagacg gacgatccgg cggtcgaagc gctgcgcgcg aggcagatca
     2881 gaaacttcgc cgccattctc atgctctcgc gcggcgtgcc gatgttcacg gccggagacg
     2941 agatccgccg cacgcagaag ggaaacaaca acgcctactg ccaggagaac gagatcagtt
     3001 ggttcgactg ggacctcgtg gagaagaact gggatatgtt ccgtttctgg agtctgatga
     3061 tcgatttcag aaagcggcac tcgacactgc acgatccgca tttcttccgg gggagggtga
     3121 acgggcgagg cgtcgcggac gtgacgtggc acgggtgcaa actgaaccag cccggctgga
     3181 acgatccggc ggcgaaggcg ctcgcattca cgctgggcgg ctttggcggc gatccggact
     3241 tgcacgtgat gatgaacatg cactgggaga ggctggagtt tgaagtgccg gcggttcccg
     3301 ggcggcggtg gagccgggcg atcaacaccg cactgccacc ccccgatgac atcgcggaag
     3361 cggggatcga ggccgccttc gatgggagcg catgcgtggt ggaagggcgc agcatcgtgg
     3421 tgctggtgtc ggcatgaggg gccgagcgcg gccgggtatc gggaaagtca tgtagaatgc
     3481 ctcactcgga ggcatttctc acgatgcgtg tgattctctc attcgttctg ggtgcgggcc
     3541 ttgcgctggc ggcgcccgcc ttgcggatca ccggcggggc cgtcgacgac caggtgttcc
     3601 agcgcggccc cggaaacacg gtcgatatcg ccctttccgg caccgcggct gacgccgact
     3661 ccgagcccat cgaggttcgc atcacgcgaa agttcattcc ggtggagggg tgggactgga
     3721 aggtctcagg ctcggtccgg caggaccagt ggagtgcgga gttgcgcggc gtgcctgccg
     3781 gcggacctta ccggatcgaa gttcgggttc acgggacgac ggtatcggac agcgtgcgga
     3841 acgtgctggt cggtgacctg tgggtcctgg cgggacagtc gaacatgcag ggcgtcggcg
     3901 acctcgtgga tgtcgaacca ccgcatgagc tggtccacaa cttcgatatg gccgaccact
     3961 gggtggtggc ggaggaacct ctgcaccggc tggtggacgc agtcgaccgt gttcactggt
     4021 ctcgcaaccc gcaaacgaag gaaccggagc ggttcgaggg cgagaggctg cggaagtaca
     4081 ttgcggagcg caggaagggg gccggccttg gactgccgtt cgcggtggag atggtcaagc
     4141 agatgggcgt gccgatcggc ctggtttcat gcgcgcacgg cgggacatcg atggaccagt
     4201 ggaacccggc gctaaaggac cagggcggcg actcgctgta tggttcgatg gtgcggaggg
     4261 tgcgtgaggt tggaggaaag gtcgccgggg tgctgtggta ccagggtgaa tccgacgcca
     4321 atctgaaggc cgcgcccgaa ttccaagcga agttcgagcg cttcatcgcc gcggtacggg
     4381 aggacttcgg gcagccaaat ctgcctttct actatgtcca gatcggccgg cacgtcgccg
     4441 atgcaaacat ggccgagtgg aacctggtgc aggagggcca gcggaacgcg gaggaaagtg
     4501 ccgcgcgctc ggggatggca ccggcggtcg atctttcgct cgacgacagc atccacgtcg
     4561 ggacgcagga cctgaaacgg ctcgggcgga ggctggcgaa tctagcgctg ggccgcacga
     4621 gacgcggtcc caggccgttg tcggcggtgt tcggcgacgg catggtgcgg gttgtgttca
     4681 gcgatgtcaa cgggcggctt acgtccaagg cgcgcatcgc agggttttcg attcacgacg
     4741 ccagcggagc gtctgtcccg ctcatctaca aggcacaggt ggacccggtg gacgggagcg
     4801 cggtgctgct tttcatcgga ggaaagctgc cggaacgtgc gacactgcgc tacggcgccg
     4861 gcagggaccc gtattgcaac ctgaacgatg aagcggacat ggcttcgccg gtgttcggac
     4921 cgatggcgat tcaatagcgg tctccggacg cctctgggta gttcgtaagc tgatacaatc
     4981 gaagatactt cacggaataa ggaccctcat caaaatgaaa cttcttttgt gtgccgggct
     5041 gctgttgccg ctgtgctcgt acgcgcaaac tgaggggacg gccaaacctg tggcgcctgc
     5101 tccggcagcg acactgtcaa cactgcctcc cagcactgtc gtagccacgg tggatgggac
     5161 caaggtgacc gcctccgacc ttcaggcaat cctgcgagcg ttgcctcccg caagccagca
     5221 gcaggcgctc agaagcccgc gacagtttct cgatcaattc ggcctgttac gtaagctatc
     5281 cgccatggca gagcaggcca aacttcatga gcgcagcccc ctcagagagc aactggagta
     5341 caaccggatg gtcggcatgg cccaggctca actgctggaa gcccagaatc aaatccacat
     5401 ttcgaccgag gacgcccaga aacagtacga ggcaaacaag gccaagtatg agcaggcgaa
     5461 ggtgaaggtc atctatattc ctttcagcgc gaacgcgccc gcggcgcagc agagcaatgc
     5521 ggaaaagaag gttctctccg aggcagaagc gaaggagaag gcggaaaagc tgcttgccga
     5581 aatcaagggc ggcgccgatt tcgtgaagct cgtgaaggag cattccggag acccggtctc
     5641 caagggtaag gacggcgact tcggcaacat taaacggagc gaccgaattc ccgaggcaat
     5701 caagacggct gtattcgcac tcaagccagg agaagtaagc gagccggtcc gacagccgaa
     5761 tgggttctac cttttccgcc tcgaggaaag cagccagcag atgttcgacc aggtaaagga
     5821 tcagatcacg gccgagatga ggcaggcccg tttcagtgag tggatgcagt ccatgcagaa
     5881 gagcatcgat atcaagatcg agaacgaggc tgcgttcgct cctgttgcgc ccgctatcca
     5941 agcggcccct ctaacaccgc ctgtgaagca ataacagatc ttgcgttcca gttccgaatt
     6001 gattgcaggt gtcgtcaggc tgccccaggg gttcgaccgg cgagtacgcc gcttgcttga
     6061 gcagacgatg gcatgcctcc cggcgttcga agggaagcag gtccgggtcg tcctgagacc
     6121 ggcgctgttc gctcaccggg gaacactgct ctcgggcctg gatctggggg ctccggtgca
     6181 tgccggatca tttctccgcc gccgtgagat tgtgctcgat aggtctttgt gtgacgagcc
     6241 ctccgaactg gcgcggattc tcgttcacga gattttccat ttcgtctggc tgcggatggg
     6301 aaaccatgtg cgccggtcgt tcgaggccaa gcttgccgcc gagcttcagc gaaacgcgcg
     6361 tggtgagctc ggctggtctg ccgatctgcg caagcaaacg ctgtcttcgg gcgatgtcag
     6421 agggcgaaac aggcgatggc gggagtacgt gtgcgagagc ttctgcgatt cagccgcctg
     6481 gctgcatacg ggagccaagg gccacgatga gttcaccctg gcgccgcgat tccggcgcgc
     6541 tcgccgggaa tggatgcgtc acttcgaggc agccgggcgc atccctatat aattgaaaga
     6601 gggagattcc tttggcatcg tcggtctcac gctgcgtgtc cgctgttgtg ctgttatcgt
     6661 gcttcttgct cgcggcttgc tcgtcgcatt ccgaaatggc aaaaacatcg gcgacttccg
     6721 ggaaggcgac gacggaagac cgcaaacccg caccggagtt ctccctgaag gacggcgacg
     6781 gccggaccgt cagtctcgcc ggataccgcg gcaaagtggt gctgctgaac ttttgggcca
     6841 cgtggtgcgg cccgtgcaag ttcgagattc cgtggttcaa ggagttcgag cagaactaca
     6901 aggatcgggg attcgccgta ttaggtgttt cgatggacga ggaaggatgg gacgtcgtca
     6961 agcccttcgt ggcggaacta caggtgaact accggattct gatgggcgat gacacgacag
     7021 cgcagcttta tggcggcgtg aactctctgc cgacgtcgtt cctcatcgat cgggaaggac
     7081 gaatcgcctc ggttcatgtg ggactggtgg gtaagggtga ctacgaagat gaaattgagc
     7141 gactccttgc ggcgccggcc agcggttctt ttcgtcgcgc tggtgttggc atgcctgcct
     7201 tcgctttggg cgcaaagtag ctccgtactc actgcggtga cgcctccccg gacggttggg
     7261 aaacgaggag acgtgttcgc cacgaaggtc accgtccagc tccggaacgg ctaccacgtc
     7321 aacagcaaca cgccggcgga cgagtacctg attcccctgc ggctcacatg gaacgcctcg
     7381 ccgcttcagg ttgtggatgt ggcatatccg aaaccgagat tggagaacta ctccttctcc
     7441 acgaagccgg tttcggtgtt taccggggat ttcgacattg tgtcccgatt caaggcgcct
     7501 ccgaattctc cacaggggcc tagcgtgctg gtcgggaaac tgcgctacca ggcctgtaca
     7561 gccaccgcct gccttgcgcc gaagaccgtc gaggtccggc ttccggttca catccagtaa
     7621 acttgcgttt tgaggcgcct gatcatacgc cccggtgcga tcggggactg cattctgtca
     7681 cttccagcga tggagcgcct gcgcgcgggc tacacagaag tatgggtcgc cgcgcagaat
     7741 tgcccgttca tccgttttgc cgacgctgtg gattcgatcg gcgccacgtc actcgacctg
     7801 gtcggcctgc cgggggtgga gccgccgccg ggcgcaatca cccggctgcg cggcttcgat
     7861 gacatcgttt cgtggtatgg aagcaaccgc gtggagtttc aggctgccgt ggccgaactc
     7921 ggcctgccgt tccggtttct gacggcgctg ccggatgaaa cgcggggcgt ccacgcggcg
     7981 gacttctatc tggagcaagc gcagagcctg acgggatgcg aaacggggga aatcgcctgt
     8041 cccactctgc ggatcgagag cctgccgcgt aatgaagact tcgtggtgat tcatccgttt
     8101 tctggcagca gccgaaagaa ctggccactc aaacgctatc gagaactggc ggaacggctg
     8161 gctgtgcaac tgtctgtccg ctggtgtgcg ggcccggagg agtcactcga aggcgcggcc
     8221 cggttcgatg atctctacga attggcgagc tggatttccc gcgccgcggt atacatcggg
     8281 aacgattcgg ggatctcgca catggcggca gccgccggcg cgcccgtggt tgcgctattc
     8341 ggaccgaccg atcccgcggt gtgggcgccg cgcggcccga aagtgcggat tgtgtccacg
     8401 cggccagccg gcatggagat ggagacgatc gaggtcgacg aggtgctttt ggctgcccgc
     8461 gaactactca gcgctcaaac ccgacagcgc tcagaatgac gccaagtttg cggtgatcac
     8521 ccgcgacgga gaatgtccgg tccgcggtga tagtgactgt cgggctatcc gacagcgtca
     8581 cgacgggctc ggaagtcagg gtgtatgttc cgggggcggc gaaggtcttg tccgcgacct
     8641 gcactccgtc gacggtaacc gtgatgcggc gggccggc
//
LOCUS       DATIFQ010000024         5126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1922276, whole genome shotgun sequence.
ACCESSION   DATIFQ010000024 DATIFQ010000000
VERSION     DATIFQ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922276"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1071)
                     /locus_tag="VLE22_00855"
     CDS             complement(190..1071)
                     /locus_tag="VLE22_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HSB12972.1"
                     /translation="MSEFKSAGAQFITVDGKYRVWTKRVGDSPIKVLTLHGGPGACHL
                     YFECFEDFLPQAGIEFYYYDQLGSLFSDNPDDPSLWTIERFCDEVEQVRLALGLDQFI
                     LYGQSWGGMLAIEYALKYQEHLKAVVISNMTASIPCYVDYLNELRRKFPEEVQRTLEK
                     YEAAGDYEAPEYQEAMYKQLYGAHLCRLDPYPAPVDLSFRFLNPRIYNHMQGPNEFVV
                     TGTFKDWDRWADLPKIHVPALVMAAKHDTMSPEELGRMAKLMPNGRLAFCPNGSHLAM
                     WDDQEVYFRELIAFLKS"
     gene            complement(1085..3178)
                     /locus_tag="VLE22_00860"
     CDS             complement(1085..3178)
                     /locus_tag="VLE22_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12973.1"
                     /translation="MKTTLTRRQFAQSALVLHAMPPASKGAGRNDTLVAFRISTYHWV
                     DDRRFDALLAFFAKYPGTADELAFFTGVTHIPRPLEEMERFSKLLEKRMTAARQAGMG
                     AGINVLATIGHLDENLETSLNESWQRVVDPRGRQPKGSYCPCGREFLGYVDKLYATMA
                     QAGPEFIWVDDDVRTMHHPPVQYACFCGDCLRRFSEQTGRNFTREALVKAFSSGTLEE
                     RLELRRQWLDHNRSGIDGVLRAVEQAVHRVKPDAAVGLMCGGSLYYDRFDHPGWTKTM
                     AGPENRPVRYRPGGGFYNEERMVEFVEKAHHLGSQIAAFPPQVQIIQSEIENFPYDLL
                     RKSAWTTAVEAATYQGAGATGTAFNVLSQRPDPLDEYEPILRRVRDAKPFYATLRAEL
                     GRSPVIGVWPAWNNDIFAANSIEGRWPDGSHDASAGVSRGFVLSELGIPVCYDRKDAV
                     VTVFSGPALLALGRDEVRTMLLRGGVLMDVAALEALESLGLGELAGVRPGKKVVKDGI
                     EQLTKHPANGKFAGYSRDCRQSFSWNEPATLLEPRSPNVTVMAKLIDYQDRELGNSMT
                     AYESSLGGRVVVAGYFPWSQLHNFAKSSQMKAVCDWLSRGRMPVVVDSFARIHVWARK
                     PGPGKLACVVLNGSQEPLQTIRLRLRGQYSKLEWVTFENRRIALKPGGGAAGEVRAST
                     PPLAAWNIGLLLASA"
     gene            complement(3199..>5126)
                     /locus_tag="VLE22_00865"
     CDS             complement(3199..>5126)
                     /locus_tag="VLE22_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12974.1"
                     /translation="APWLTRSSDSYILLLGSLKLNMIRILSALLFLLPCLPAAAQTRR
                     IVFDSAAPVSEHKWTLKELGLPSALGAGKFLVLELRASSPQRFRLKLYAPDGEAAVLL
                     HLYPNAWIRTAIPLALFTSPPREGIDMAAVYNKSRLGYYINLGGPYRSLESVEALGFV
                     MQEPLASPVLEIRSIRVADKSPGDAVLEREPLVDEFGQYIRADWPGKATTLEQLQNAW
                     KREEESLKPGEFGYCKYGGYLETKAKATGFFRVEETGGRWWLVDPDGHLFFSVGSDVV
                     TPWMNTPTEGRSGVFKALPPTEIGPPRIGRRREPGTSFYAWNLFRRFGPEWPARWVDV
                     SIRRMQAWGLNTVANWSDAKLWDAGRIPYTIPLRDWQTKVSYMGLPDVYSEEFVRNAD
                     RAAAAQCAPRKDDPWLLGYFLANEPPWPGREGIVIEMILSGPGTATKQELKRYLAQGD
                     TPERRRGFVYNAFEKYIEVVNAAVRKHDPNHLNLGMRFGGHPPEEMSRAARAFDVYSI
                     NIYDYSPGREAIDRANALAGRPVMIGEFHIGTPGRGLSAGLRQARDYKERGVAYQYYV
                     ENAAAMPAMIGAHWFQWADEPATGRGDGENYNIGLVDVTDRPYDDMIESLRTTHKRLH
                     AVHSGKEPAVDRRPIVH"
BASE COUNT          935 a         1596 c         1614 g          981 t
ORIGIN      
        1 gccggcagcg ttggaggacg tgtcggacct cgtgccggag aggagccgcg aacactacga
       61 cgacttccgc tgctgcccgg gctgccatcg cgtgtactgg ggcggttctc actaccgcag
      121 gatggaacgg ctgatcgagc gggccgtgga agaggtacgg agcggcgcct gaaagcccga
      181 gcgcgaggct cagcttttca ggaacgcgat cagctcccgg aagtacacct cctggtcgtc
      241 ccacatcgcc agatggctgc cattcgggca gaaagcgagc cggccgtttg gcatgagttt
      301 cgccattcgt cccagctcct ccggactcat cgtgtcgtgt ttggcggcca tcaccagcgc
      361 gggcacgtga atcttcggca gatcggccca ccggtcccaa tccttgaacg tgccggtgac
      421 gacgaactca ttcggcccct gcatgtggtt gtagatcctg ggattcagga atcggaagct
      481 caagtccact ggagcgggat agggatcaag gcggcacagg tgcgctccat agagctgctt
      541 gtacatcgcc tcctggtact ccggggcctc gtagtctccg gcggcttcgt acttctcaag
      601 cgttcgctgg acttcctccg ggaacttccg ccgcagctcg ttgaggtagt ccacgtagca
      661 cgggatgctg gcggtcatgt tggagatcac caccgccttc aggtgctcct ggtatttcag
      721 ggcgtactcg atcgccagca tgccgcccca agactggccg tagaggatga actggtcgag
      781 gccgagcgcg aggcggacct gctccacttc gtcgcagaat cgttcgatgg tccagagaga
      841 gggatcgtcc gggttgtccg agaacagcga ccccaactga tcgtaatagt agaactcgat
      901 gccggcctgc gggaggaaat cctcgaagca ctcgaagtaa agatggcacg cgccagggcc
      961 gccgtgcagc gtgagaacct tgatcgggct gtctccaacg cgcttggtcc agacccgata
     1021 cttaccgtca acagtgatga attgcgcacc tgcacttttg aattcactca tggaaacctc
     1081 cttctcaggc tgacgctaaa agcaatccga tattccacgc tgccagcggc ggggtgctgg
     1141 cgcgcacctc cccagccgct ccacccccag gtttgagtgc tatgcgccgg ttctcgaatg
     1201 tcacccattc gagcttcgaa tattggccac gaagacgcaa tctaatcgtc tggagcggct
     1261 cctgcgagcc attgagaacc acgcacgcca gtttgccggg acccggcttg cgagcccaca
     1321 catggatccg ggcgaaggaa tcgaccacga cgggcatccg gccgcggctc agccaatcgc
     1381 agaccgcttt catctgggat gatttcgcga agttgtgtag ctgcgaccac gggaagtagc
     1441 ccgccacgac cacccgccct cccagcgagc tctcatacgc ggtcatcgag ttgccaagct
     1501 cacggtcctg gtagtcgatg agcttcgcca tcacggttac gttcggcgag cgcggttcga
     1561 gaagagtcgc cggttcgttc caggagaacg actggcggca atcgcgggaa tagccggcga
     1621 actttccgtt ggccgggtgc tttgtcagtt gttcaatgcc gtctttcaca accttctttc
     1681 ccggccgtac cccagccagc tcgccgagtc ccagggattc cagcgcctcc agagcggcca
     1741 cgtccatcaa cacaccaccg cggaggagca tcgttcggac ctcgtccctt cccagagcca
     1801 acagcgccgg acccgaaaag acagtgacca cggcatcctt gcggtcatag cagaccggga
     1861 tgccaagttc ggaaaggacg aatccgcgtg agacgcctgc cgaggcgtcg tgcgacccgt
     1921 cgggccaccg gccctcgatg ctgttcgcag cgaatatgtc gttgttccag gccggccaga
     1981 cgcctatgac gggactgcgg cccagttcgg cgcgcaaggt tgcatagaac ggtttcgcgt
     2041 cccggacgcg ccgcaggatt ggctcgtact cgtcgagcgg atcggggcgc tgcgagagga
     2101 cgttgaacgc tgtcccggtc gcacccgcgc cctggtacgt cgctgcctcc accgccgtcg
     2161 tccacgcgct cttgcgcagc aggtcgtacg gaaagttctc gatctcggat tggatgatct
     2221 gtacctgcgg cgggaatgca gcgatctggc tcccgaggtg gtgtgctttt tcgacgaatt
     2281 cgaccatccg ctcctcgttg tagaatccgc ctcctgggcg atagcggacc ggccgattct
     2341 ctggcccagc catcgtcttc gtccagcccg gatggtcgaa ccggtcgtag tacagacttc
     2401 caccgcacat cagtccgacg gcggcatccg gtttcacgcg gtggacggct tgctcgactg
     2461 cccggagcac tccgtcaatc ccgctccgat tatggtcgag ccactggcgg cgcaactcca
     2521 gccgctcttc cagggtgccc gaactgaagg ctttgaccaa agcctcgcgc gtgaagttcc
     2581 ggcccgtctg ttccgagaag cggcgcaggc aatccccaca aaaacaggcg tattggacgg
     2641 gcgggtggtg catcgtgcgg acatcatcgt ccacccagat gaactccggc cccgcctggg
     2701 ccatcgtggc gtagagcttg tccacgtacc cgaggaattc ccggccgcac gggcaatagc
     2761 tgcctttcgg ctgccttcct cggggatcca cgacccgctg ccaggactcg ttcaacgagg
     2821 tctccaggtt ctcatcgaga tggccgatgg tggccagcac gttgattccg gcgcccattc
     2881 ccgcctgccg tgcggctgtc attcgcttct ccagcaattt cgagaagcgc tccatttcct
     2941 cgagcggccg cgggatgtgc gtgacacctg tgaaaaacgc gagctcatcg gcggtgccgg
     3001 gatacttcgc gaagaaagcg agcagcgcat caaaacggcg gtcgtcgacc cagtggtacg
     3061 tcgagatgcg gaatgcgaca agggtatcgt tcctgccggc gcctttcgat gccggcggca
     3121 tggcatgaag gacgagagcg gactgtgcaa actggcggcg cgtgagagtt gttttcatgc
     3181 ggcttctcct gacatcgatc aatgcacaat gggcctgcgg tcgaccgccg gctccttgcc
     3241 cgagtggacg gcgtgcaagc gcttgtgagt cgtccggaga gactcgatca tgtcgtcata
     3301 ggggcggtcg gtgacgtcga caagaccgat gttgtagttt tcaccgtcgc cgcgcccggt
     3361 tgccggctcg tcggcccatt gaaaccagtg agcgccaatc atggcgggca tggcggcggc
     3421 attctcgacg taatattggt acgcaacgcc gcgctcctta taatcgcgcg cctgccggag
     3481 cccggccgag aggccgcgtc ccggcgtgcc gatgtggaat tcgccgatca ttaccggccg
     3541 gccggcgagt gcgttagcac ggtcgatggc ctctcgcccg ggagaatagt cgtaaatgtt
     3601 gatgctgtag acgtcgaagg ccctggcggc acggctcatc tcctcgggcg gatgcccgcc
     3661 gaagcgcatg ccgagattga ggtggttcgg gtcatgcttt cgcaccgctg cgttcaccac
     3721 ttcgatgtat ttctcgaagg cgttgtagac aaagccacgc cggcgctctg gcgtgtcgcc
     3781 ctgcgccagg tagcgtttca actcctgttt tgtcgcggtg ccgggtccag agagaatcat
     3841 ttcgatgacg atgccctcgc gtcctggcca cggcggctca ttggcgagga agtagccgag
     3901 aagccacggg tcgtcctttc gcggcgcgca ctgtgcggcg gcggcgcgat cggcattgcg
     3961 gacgaactcc tccgagtaga cgtcgggcag gcccatgtag ctgaccttcg tttgccagtc
     4021 acgcaggggg atcgtgtacg gaatgcggcc cgcgtcccag agcttcgcat cggaccagtt
     4081 ggcgaccgtg ttcaagcccc aagcctgcat acgccggatg ctcacgtcga cccaccgggc
     4141 gggccactcc gggccgaaac gccggaaaag gttccaggcg taaaacgacg tgcccggctc
     4201 gcggcgcctg ccgattcggg gaggtccgat ttcggtgggt ggcagcgcct tgaacacgcc
     4261 gctgcggcct tcggtcggag tgttcatcca gggagtgacg acgtcggagc cgaccgaaaa
     4321 gaacagatgg ccgtcgggat cgaccagcca ccagcggccg ccggtttcct ccacgcggaa
     4381 gaagccggtt gctttggcct tcgtctccag gtagccgccg tacttacaat agccgaattc
     4441 gccgggcttg agcgactcct cctcccgttt ccatgcgttt tgcagttgct ccagcgtggt
     4501 tgccttgccg ggccagtccg cgcggatgta ctgtccgaat tcatcaacca atggttctct
     4561 ttcaaggacg gcatcacccg gggacttgtc tgctacgcgg atcgagcgga tctcgagcac
     4621 cggagaagcg agcggctcct gcatgacgaa tccgagcgct tcgacggatt ccagggaacg
     4681 gtagggtccg ccgaggttga tgtaatagcc gaggcgagac ttgttgtaga ccgccgccat
     4741 gtcgattccc tcgcgtggcg gcgaggtgaa gagggcgaga ggaatggcgg tccggatcca
     4801 ggcgttcgga tacaggtgca gcagaaccgc tgcctcgccg tccggtgcgt acagcttcag
     4861 ccggaaccgc tgcggcgagg acgccctcaa ctcgagtacg aggaacttgc cggcgccgag
     4921 cgcggacgga aggccgagtt ccttcagcgt ccatttgtgc tcggagaccg gcgcggcgct
     4981 gtcgaatacg atgcgtctgg tctgcgcggc cgcgggcaga cacggcagga gaaagagaag
     5041 tgctgagaga atacggatca tattgagctt aagactcccg agtaggagta tataggagtc
     5101 cgaactccgc gtcagccaag gtgcgc
//
LOCUS       DATIFQ010000025        28084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2114106, whole genome shotgun sequence.
ACCESSION   DATIFQ010000025 DATIFQ010000000
VERSION     DATIFQ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 28084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28084
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114106"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VLE22_00870"
     CDS             <1..1039
                     /locus_tag="VLE22_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HSB12975.1"
                     /translation="TMAALWPLAEAARFAGIDLYTDRYRSLYDAPLALALPNGEPPGF
                     NDSAGGNVAGYAPLYEIAYARYRVSAYGALVSRTQRDSVQALLCGEETASTGPMIPER
                     SALLPSAGFAMLRSSAMTVAVRFGRHGGGHGHPDKLNIVTFAGGRVFGLDPGSINYGV
                     PLHREWYRSTIAHNTVGADGQIQASADGRHEAWETGGGITRFTAIADKVYPGVTLRRS
                     LALYGSRLDDRFECLAGAEHDYDWAFHAPGQLRVSVKLSERRGALGAENGYQHIRGVS
                     EGRPDGDWTARWESGDAVLTLRFRTASGTMVFTGAAPGRNPAELVPMLVVRRRAANTV
                     FEVTHEISARS"
     gene            complement(1049..2065)
                     /locus_tag="VLE22_00875"
     CDS             complement(1049..2065)
                     /locus_tag="VLE22_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12976.1"
                     /translation="MTAARHLEKYGPGLQTVALHTSCEPAVLLELMRTGVREFLYPPF
                     STPALREALARVREIAERMPPPVESTDLVFTFLPSKPGVGTSTVALNVSAAMATVQDG
                     RVLIADFDLNSGLIGFMLKLDSPYSLVHAAESSANLDESLWRQVVSSFGRLDVLTSGK
                     LNPGFRIEPVQSRALMDFARRHYKAICVDLSGNMEKYSIDLMHESKRIFLVCTQELPS
                     LHLAREKLSYLRTLELEERVSLVLNRTHKRAIIGPEEVEELLGHSVNLIFPNDYAGAH
                     HALAAGKCVDPSTELGRHFRNMAEGILVKRQIGTESKNRFVEYFSLLPARYSLSHAAK
                     KSGD"
     gene            complement(2360..3259)
                     /gene="truB"
                     /locus_tag="VLE22_00880"
     CDS             complement(2360..3259)
                     /gene="truB"
                     /locus_tag="VLE22_00880"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HSB12977.1"
                     /translation="MLQLRLMDGVIVADKPEGWTSHDAVNKMRRLADTRRVGHLGTLD
                     PMATGVLPLVIGRATRLAQFYMRGDKTYDAVIRFGYSTDTYDRDGKPTSPKTEPEINR
                     VRLEEALNSFRGLIHQTPPPISAKKIGGTPAYQLARKNLPVNLKPVEVTIRNIELAGV
                     DGSRARVVVECSAGTYMRSMAHDLGEILGCGAFLEGLRRTVSGEFSLQHARTIDELAA
                     LSAEKRLGEALVPAAQLLPEFPCEIVDRLTAGQIRQGRDFRVSPFRTRTVTKYVKAVT
                     QEGELVAIGEVRLPNLYHPCVVL"
     gene            3308..5134
                     /locus_tag="VLE22_00885"
     CDS             3308..5134
                     /locus_tag="VLE22_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB12978.1"
                     /translation="MEEEVLGKAYDGRLMRRLVKYMRPYKGVVFLSLLFLLLHSVLQI
                     LGPLLTKVAVDRYLAPTPGSLPAPFESYLSADPWTGITQISVLYLLVVLGTLVAQFLE
                     TVLMQWTGQCAMFDLRRELMTRLQQLDIAYFDRNPVGRLVTRVTTDVDVLNELFASGL
                     VTILGDLLMLSFVAVAMFKLSPALTLVLLGVMPLVVLVTAVFRRTITKAYRQVRIAIA
                     RINAYLQEHVNGISVLQLFNREKKSGEEFESINRTHMDAFKDAILAHGWFYPVVEFLG
                     MLALAMLLAYSGYKVESGELTLGIMVAFFQYGLRFFRPIQDLSEKYNILQSAMAASER
                     VFKLLDTPVRIAVPPAPKPFPTGQVRIEFDGVWFAYKEEDWVLRDVSFTVEPGETVAV
                     VGHTGAGKTTLINLLLRFYDVQRGAIRIGGVDVRECDPHDLRHHFGFVLQDPYLFSGT
                     IEDNIRLGTGGITRDDLEEAAEQVNLIEFIRSLPDGFTQPVRERGSGLSTGQKQLINF
                     ARALAHNPQCLVLDEATSSVDTETEIKVREALSRMVEGRTSIIIAHRLSTIQRADRIL
                     VMHKGRLRETGTHQQLLALKGIYWHLYQLQYKDQEIREPKAV"
     gene            complement(5254..5757)
                     /locus_tag="VLE22_00890"
     CDS             complement(5254..5757)
                     /locus_tag="VLE22_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12979.1"
                     /translation="MTIHGFTISAFGHCASKQNFDSSKLARNAVTYLMSVLDSHGGNF
                     NLKLPMKGVGEVELQWTSEHLSWAMASFINKDQLLSTLVMVSGINLEADQHALEMAQA
                     ALEDVCRAAGEQPQDDLLQIAERPSVATIRWATSDRRTLDLITDMETCLAAAFLERAF
                     DAGSRAL"
     gene            5862..6995
                     /gene="ribB"
                     /locus_tag="VLE22_00895"
     CDS             5862..6995
                     /gene="ribB"
                     /locus_tag="VLE22_00895"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HSB12980.1"
                     /translation="MPFCSILEAIEEIRAGRMLVVVDDEDRENEGDLTIAAGMVTPEI
                     INFMATWGRGLICLALDAERSDFLQLPPISPRNTSRFGTAFCESIDAREGVTTGISAA
                     DRALTIRVAVDPATKPRDLARPGHVFPLRAREGGVLVRAGQTEAAVDLARLAGLTPAG
                     VICEIMNDDGSMARVPQLEEFCRRHGLKMISVANLIRYRLETERLVRRVAEGCLETEF
                     GPFRTIAYASSIDPEMHLALVHGDVAGTERVLVRMQARCVYGDVFGSTQCDCHRLVHT
                     SLARIAREEGGVLVYLHHTGLGIRIDKDPDGRGRLSCHDRDFMHYKTADGQRQLQYES
                     GLGAQILSDLGLHTIRLLTNHPRKIVGLEAFGIELVEQVPVAL"
     gene            7047..8171
                     /locus_tag="VLE22_00900"
     CDS             7047..8171
                     /locus_tag="VLE22_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12981.1"
                     /translation="MRSSTGSALILLLAVAIVPAADTNLMNLVMPDARMISGFNIERA
                     KASPFGQFLLSQMAASDPDFEKFVAATGFDPRRDLHEMLFASAGTPDSAHAIVLVRGL
                     FDSPRIEAMAKLNGAIIRAYKGVAVMHGTQRGGPWSFNMGATKADGAFAFLDNTTAVL
                     GDARSLHSVIDRRVGGRGLNPRLAARAQQLSEQYDIWGVSMMPVSEWASKVPDENVSG
                     VMQGDALRSIEQSSGGIRFGKVIEISGEAITRSEKDATALADVIRFLTGLLQLNQKDA
                     RITGPAKLLQSLDLRTEGNVMKLSLLVPEAELEKFILEAKAQAQKTAAARKTRAPSGT
                     LAPSATQPSSGMQPPPRRPAPAAQSGGLTIHSSEGTIQIK"
     gene            8245..10101
                     /locus_tag="VLE22_00905"
     CDS             8245..10101
                     /locus_tag="VLE22_00905"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSB12982.1"
                     /translation="MKPRTTHFLVLLSFLLLPAVSPTVAQPKARHAFALGKDDFLLDG
                     RPFQIRGGEIQPNRVPCEYWRHRIRMAKAMGLNTIASYVFWSYHEVEEGRFDFKTWNR
                     DIGEFFRIAQEEGMWVFLRPGPYCCAEYDFGAIPTYLLRDPELKIRCMYPRYTQAAER
                     YLRALAAVVKPYQITEGGPILMIQLENEYGSYANDRIYMKWLHDLWRKLGITVPFATG
                     DGATPHMLEAGSWPGAAVGLDPGSQLSHWDLARRMNPGVPIFSSETYPGWLTHWGEKW
                     AHVSVESISKEVRFLMDNGKSFSLYVVHGGTSFGFIAGANADPLPESQNGIASSFMPD
                     VTSYDYDSPITEQGRPTPKYFALRDLIASYLKGEKLAEVAKPIPALSIPPIRLERFTT
                     IWDNLPSPVKLVQPKPFEALGQYQGLMLYRTKLTGHKSGRLLMRNLHDYALVFVDGIL
                     IGSVDRRLNQNSIMLPKTESKEPVLEILVEAMGHINFGEFIIDRKGITDRVLLNNMTL
                     MNWETFGFPLEEQWVQSLKRTTTPAARPGVFLKGHFRLDKPADTFLDMTGYRKGYVWV
                     NGNNLGRYWEIGPQKRLYCPACWLKPGDNEIVVLDLHQAEPKPIAGHEKLDD"
     gene            10113..11081
                     /locus_tag="VLE22_00910"
     CDS             10113..11081
                     /locus_tag="VLE22_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB12983.1"
                     /translation="MQHIALRHTDLTVSRVCLGTMTFGGQTDIDEAARLVDHCLDSGV
                     NFFDTANIYNKGASETILGRILRGRRSGVVLASKVRGKMGDGPNESGLSRVAIMRALD
                     DTLRRLGTDYLDLYYLHQPDYDVPLEESLDAIDRLVRLGKVRWPASSNYASWQVCRML
                     WLAETHSYKPACVTQPMYNVLARGIEQEYLPMAKEFGVSTVVYNPLAGGLLTGKQQRE
                     APIPGTRFDNNQLYLDRYWHPAYFDAVDRLGAIAASVGRSLVSLALNWLLHHSPADCL
                     ILGASRLDQISENLRAAEEGPLPEDAVAACDQVWADLRGITPKYNR"
     gene            11099..12589
                     /locus_tag="VLE22_00915"
     CDS             11099..12589
                     /locus_tag="VLE22_00915"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HSB12984.1"
                     /translation="MPSSLRGLAVLFSLCALAPAQNTLPLFQEGRIRTLIFSGRNNHD
                     WRTTTPALKKYLLDTGRFDVRVVEEPAGTTAETLAAYQVIVLDYNGPRWGAGTEKAVE
                     DFVKSGGGLVAVHGASWAFSGLPVLGDRHVRINLVEPAWAEYARMVGGIWSEQEPKTG
                     HGLLHSFKVKFIDPSHPITQGMGESFLATDELYHNMRMQPGAKILATAYDDPKMKGTG
                     KDEPILWTVDYGKGRVFHTALGHDLASMMEPGFVTTFVRGTEWAATGAVTLPAAIDPA
                     RPAADRLRVLVVTGGHSFDTSFYTLFEGYPDIAWDHSVTSHEAFREDIRPKYDVVVFY
                     NMHDKPEDITEAEKKNLVDFAQSGKGLVVIHHAIANYPAWDWWSKELVRGKYLLKPEG
                     GMPGSTYKHDVEMFVEPVGQHPVIAGASRMHIWDETYKGMWIAPDVQVLLRTGEPTSD
                     GPVAWISPYSKSQVVYIQLGHDRLAHIHPGYRRVIKNAIDWAGGRK"
     gene            12586..13914
                     /locus_tag="VLE22_00920"
     CDS             12586..13914
                     /locus_tag="VLE22_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB12985.1"
                     /translation="MRSDFTRRDMLRLAGAGVLGDLALPAQTRRPNLLFILADDLGWG
                     DVGFNGRKEWPTPNLDRLASQGTTFTRWYSAATVCAPSRACLLTGKYTIHNGVKRNGD
                     DLPASEVTFAEALKPQGYTSALIGKWHHGALRDGSFLHPLDQGFDSTFGFLDARHAWE
                     HFPKTLFRGRQEVPVKGYTADLFSDEAVKFIGENRSRPFCLYLAYIESHFHVEAPEDE
                     VAKFRGKFPEKDQRTPRNAAYAAMIHRMDTGIGRVMRALEENGLAQNTLVVFTSDNGA
                     TFESGNNGASNFHDSNRPVRGQKRSLEEGGIREPAFVWWPGRIPAGRKFEEPVHSTDL
                     MPTLVNAGGGTIDPAWKVDGINLLDVWTGKAHLPERTLFWEWTSEGYNMHAAMRGDFK
                     LLRINGADFLYNIREDPQERRTIAAAHPEMFRRLRAELEAWKASEVPRAR"
     gene            complement(13928..15097)
                     /locus_tag="VLE22_00925"
     CDS             complement(13928..15097)
                     /locus_tag="VLE22_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12986.1"
                     /translation="MSATPGYISAPGVEEADRITRIADSVIRNLEITHCYHRLSRVFS
                     SRTGPCANWCTFATWASRQAGRTIRAEDLHREFERRLVLGAKFLHPVQSLWRVLLRRG
                     LLDPATRLGKVVQEIHSPFDAFERASSAVSRGNCKVFDEIGREFAHFVHVCAPNVAPE
                     SEELQNFHKGLRPGPPPEGQDLLRQAFFHYQQQRTAASPKVKTEFVLLANLEIGLHEQ
                     TRLQPEIQEALEATLVTAQDLGKRVVEALFPGAAGWREMFRRPVVAVLERLGRSVERF
                     AVQLSRQVVTDSLMVLAIPGTVLALGKHLNEPIPEVLRDVENPELMSLLEKFEPSAGT
                     PDDCGAKDWSELHQRMHYIAHLFRAYHERPELFEPPFAPEQVRCFQSGTVPDGEL"
     gene            15199..16200
                     /locus_tag="VLE22_00930"
     CDS             15199..16200
                     /locus_tag="VLE22_00930"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HSB12987.1"
                     /translation="MRIETRLLATLLGGAIAIHAAGIVFEGSKGPGKGKHIVLVSGDE
                     EYRSEEALPQLAKILSTRHGFRCTVLFAIDPSDGTINPNRNDNIPGLETLAAADLMVI
                     FTRFRDLPDVQMKHIVDYVESGRPIVALRTSTHAFDLKTSKTYQQYGWNSKEWDGGFG
                     RQVLGETWIRHHGQHGKQSTRGIIAKGQENHPILRGIRDGDIWGPTDVYAVRLPLPGD
                     STPLVLGQVLEGMRPDDPPVAGPQNNPMMPVAWTKTYTSARGKKARVLTTTMGASQDL
                     LSEGMRRLLVNACYWAAGLEKEIPAKSNVDLAGEFSPLPFQFGGFRRGVMPRDLETR"
     gene            complement(16219..16578)
                     /locus_tag="VLE22_00935"
     CDS             complement(16219..16578)
                     /locus_tag="VLE22_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12988.1"
                     /translation="MRRRSQWTLAVAAGVALFLTGCGTVKIGRVLSDPYHYHNRNVRV
                     EGNVTHSFGAFTAGGYQIDDGTGKIIVISNRGVPSKGARVKVSGTVTSGVTVMGRSFG
                     TAIRENKHKLTGFVPPG"
     gene            complement(16652..18013)
                     /locus_tag="VLE22_00940"
     CDS             complement(16652..18013)
                     /locus_tag="VLE22_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YrzE family protein"
                     /protein_id="HSB12989.1"
                     /translation="MRERRFFLLPLLVSMVASLPAQRESGQAVKLDLKVEGKRFTRIA
                     PVTFTVSLLDINNRPAKTAVHTPIQISLRTSPKDAVQLTTVTVRRGQSSVSGRLSGLP
                     LGGVVYLWAKSPGLLAGGVSLKVRGGAPISKPAPSLPPLQAEPGPPGELPLIPVALSF
                     SPQRDFWANGKDPVTLSAFYMGPGAGSPSEIKLRFADGTGTLNPNPLVIPPGGDEGHA
                     KLTFDHAGPVEVKFLRADPQVALEGGSGFRFNFVPPPVTQLRVSVSPPAITLAETASV
                     IVELLDDDRRPAASATDTPVTLRIEQGRGSLAQTVISIPRGKSSAQTEFRPVWRGATA
                     IAVSAAGLDSQAGAVTVTLPVVLMLLSALGGLAGGFLAALPRQRSRKGWWRDPKVPIR
                     LVVGMLTGFFLYWAASLGLVSLVPRTVLMNPISAVVVAMIGGWLGTGVFSLIIKKPDE
                     AKP"
     gene            complement(18072..18803)
                     /locus_tag="VLE22_00945"
     CDS             complement(18072..18803)
                     /locus_tag="VLE22_00945"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HSB12990.1"
                     /translation="MDKVTTLRNEIRKYCAAHADAAQAGRYARYFTEGYDAWGLMGNK
                     HALWTEMEPEWIERYGGIGLLGFLKLGEMLFRDSKYEEGALAIRFVKHFRDEIDDKAL
                     DGLAKWFAAGIANWAHTDVLCGEILAPLLEGGQTELHALASWREAQYKYQRRAVPVAM
                     LGLLKTKTSARPLLEFLRPLMMDAEKVVQQGLGWFLRETWKKQPKPVEAFLLEWKDRA
                     PRVIYQYATEKMTPDARARFRRARK"
     gene            complement(18842..19969)
                     /locus_tag="VLE22_00950"
     CDS             complement(18842..19969)
                     /locus_tag="VLE22_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12991.1"
                     /translation="MPLWTPEVIRVPDGGTGLDALLETVPAQPAVFAVWPREGSPYLA
                     RTALLRRRLHRLLRERAHPSRLLNLRSVAARIEYRLTASWLETNLVFHEAAHLHFPET
                     YLELMKLRMPPYVKIVLSNPFPRSHVTNRLSGAHALYYGPFRSRNSAEQFESQFLDLF
                     QMRRCQEDLEPSPSHPGCIYGEMSMCLRPCQQVVSADEYRSETERVTEFLSSDGRSLL
                     DPAVHARDRLSGELNFEEAARQHKRVDKIQQVLKLRDELARDISRLNGVAVTPSVEPE
                     CVELWFVLGGCWQPPRRFGFEVADGKTVSMDHRLHEIAESIEPRKLARSERQEHLALL
                     ARWFYSSTRDGEWLPFDGLETLPYRRLVNAISRVAHPAERN"
     gene            complement(20034..21392)
                     /locus_tag="VLE22_00955"
     CDS             complement(20034..21392)
                     /locus_tag="VLE22_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSB12992.1"
                     /translation="MKTFSLSRRNYLELMGMGAVAAASTGTIGCQKNETKPAQPAVVT
                     AEASPPDHERRIQWWREAKFGMFIHWGLYSLLGRHEWAMEEEGIPVAEYEQLAKRFKP
                     KPDAARDWAKLAKRAGMKYMVMTSKHHEGFCHFDTKLTNYSAPKQAAGRDLAKEFLDA
                     ARAEGLRCGFYYSLMDWHHPDGARCATDEASRRRFVDYIHGQIRELCTDYGKLDVFWY
                     DMPVPLDAKGWESQKMNDMVLQLQPDIIVNNRNLLAGDFGTPEQEIRAENRDWEACMT
                     MNQSWGYHKADDNWKSPKTIVRNLVACARDGGNYLLNIGPMPDGSIPEESVRILNAVG
                     AWTEKNGTSIYDAERCRVKNSLFANFTRKGNTLYIHVHFWPGETVSIGGLQTKVKSAR
                     LLASGRPVSVKQEEFRVQFTGLPATAPDNPVTVIVAECESEPRQDVDNIRRNRPRGQV
                     GI"
     gene            complement(21483..21791)
                     /locus_tag="VLE22_00960"
     CDS             complement(21483..21791)
                     /locus_tag="VLE22_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12993.1"
                     /translation="MSAFSALLSNIVSNVPAVLVFRPVVPRLSDPHLGWLTLAMSSTL
                     AGNLTLLGSVANFIVVQRARHDVRISFWDYCRVGIPLTLLSLAAGAAYLRYVGPGRTF
                     "
     gene            complement(21788..22705)
                     /locus_tag="VLE22_00965"
     CDS             complement(21788..22705)
                     /locus_tag="VLE22_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HSB12994.1"
                     /translation="MIALAIFCGTYLVLAIGTFPGLRIDRTGAAIIGASLMLGCNVLT
                     FEEAIQAVDYSTLVLLFGMMIVVANLRLSGFFRLVSTWAVRHAHHPWTLLATIVLVSG
                     FFSAFFVNDTMCLVLTPLVLEVTATLRRNPLPYLLAVAMASNVGSVATITGNPQNMMI
                     GSLSSIPYRQFTAALAPVAIVGLLLTFAAIVLFFPTEFRDRQRVEMAELRVRVNRVLM
                     WKAVVASVGMIVFFFWGWPVAKVAIVAGALLLITRRVKPEKVYHEIDWSLLAMFAGLF
                     IVVAGVEKTSLERDLLAFAARFRLDHVGC"
     gene            22797..22922
                     /locus_tag="VLE22_00970"
     CDS             22797..22922
                     /locus_tag="VLE22_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB12995.1"
                     /translation="MHTRTELIARRDGVDYVRCLDCDQVFESDDLEPVPVTGEDE"
     gene            23174..23704
                     /locus_tag="VLE22_00975"
     CDS             23174..23704
                     /locus_tag="VLE22_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB12996.1"
                     /translation="MAETRDRNPVASPLNSKEQAALFEKAVALFHAREFAKARVLFER
                     AADGPVIEMAHSARVRARICEQRLSRTPPALSTAEEHYDYGIALINQRQLDAARRHLE
                     QAASMNPGADHVYYALALCHALKGEADAVYANLSRAIEIDPRNRGRALRDPDFGDLAR
                     RAPLSDLLYPEKERTA"
     gene            23751..24794
                     /locus_tag="VLE22_00980"
     CDS             23751..24794
                     /locus_tag="VLE22_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HSB12997.1"
                     /translation="MTRQNAPLRIVAIGGGTGLATLLRGLKPYSLGSGTVPSPTEAGR
                     AVEITAVVTVTDDGGSSGRLRREFDVPPPGDIRSCMVALSEDEALLSRLFQYRFRTGR
                     GLKGHSFGNLFLTALSEVTGDFAQAVKVSSEVLAIAGDIHPSTGANVTLQARLADGTV
                     VCGESRISRSRSPIESVFLEPRRCRPLPETCDAITRADIITLGPGSLYTSVIPNLLVE
                     GIPEAIKRSHAIKAYVVNLMWQPGETIDYSAADHIAAIHEHARMRLIDCVLVNTRPVS
                     PVLRRRYAREHARPVESDLARLREMGLTIVEGDFLEESDKVRHNSEALAEAVLKLAGE
                     RQSRRLTKRAATR"
     gene            24809..26215
                     /gene="glmU"
                     /locus_tag="VLE22_00985"
     CDS             24809..26215
                     /gene="glmU"
                     /locus_tag="VLE22_00985"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HSB12998.1"
                     /translation="MDIPVTVLILAAGLGTRMKSKKAKVLHRAGGMALIEHATDAALK
                     LTPPERVFIVVGHQADEVMAAVGDRGVRFVQQAEPKGTGHAVMAAREALEGAGGLLLV
                     LYGDAPLLTADTLLRLVARQQQGDVAATVLTTMLDDPSGYGRVLRDEHGRVRAIVEQK
                     AASPAQLAVHEINSGIYCFDSGPLWKHIGEIKPENPANEYYLTDIVGILHGKGHAIAP
                     LLREDPRELLGINTRVELAAVDRIFRDRTIRRLMLDGVTIKKPETVTIDSGVSIGMDT
                     VIEPFAQVLGRTTIGEDCVIGACSIIRGSQLGDGVVVEPFTLVLDSAFEPGARVGPFA
                     RVRMESHVGREARVGNFVELKKARLGAGAKSLHLAYLGDTEIGEEANIGAGTITCNYD
                     GVRKHRTKIGRGVFVGSNATLVAPVVIGDGSYIGAGSVVTENVPEDALALGRARQVNK
                     AEWAKKRRERKLRPDTQG"
     gene            26295..27440
                     /locus_tag="VLE22_00990"
     CDS             26295..27440
                     /locus_tag="VLE22_00990"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSB12999.1"
                     /translation="MNGLAQFDSRVPLRCCLAVVALLLSLPPMQAAEPKADEIDRLMQ
                     AYTNTVGFQGAVLVADRGTVILKKGYGLANVELDVPNTPETKYRLGSLTKQFTAMLVL
                     QLVAEGKVRLESKLSEYVPEYPKATGERITIHQLLTHQSGIPNYITPEFLAKRSREFF
                     SPTDLAKVFWERPLDFEPGAKYSYTNSGYHMLGIVVEKVTGKSYEQALRERILDPLDM
                     RDTGYDHTQAILPRRAAGYTKTPKGLQNAAFGEMSIPYAAGGMYSTVLDMRKWDAALY
                     TEKLLDRKHLDLMFQPAVKIGAGRAYGYGWMLSVLTLAGSGQKLPVIEHFGGIIGFTS
                     MIVRLPEERSLIVALANILGVEWGGATRGIAQILHGEQPETPKKPTR"
     gene            complement(27457..>28084)
                     /locus_tag="VLE22_00995"
     CDS             complement(27457..>28084)
                     /locus_tag="VLE22_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13000.1"
                     /translation="TVHEYFNTGGLAGGKMIGWPQCKEFIPLDFSRVEFPGGYPNDAG
                     VNFQHDDFFGRRQPETQALFDLTARERPDLIMNMHTGAVFPIMLRPFVEPDLVPVFEQ
                     LVRRVQGRLALDKYQATEDPAKEGNPDRVKNISPYNLDTALNLHCGALSVVIESPSHG
                     FSTAKRQDKLVVFTPDDLVNIQLLCHLEAMKFLGETGGRARWTSSNRR"
BASE COUNT         5388 a         9211 c         8324 g         5161 t
ORIGIN      
        1 cacaatggcc gccctctggc ctcttgccga agcagcgcgc tttgctggta tcgacctcta
       61 caccgaccgc taccggtctc tctacgacgc cccgctggcc cttgcgctcc cgaacggcga
      121 gccgcccggg ttcaacgaca gcgcgggcgg caacgtcgcg ggttacgccc cgctgtacga
      181 gatcgcttac gcgcgttatc gggtttccgc gtacggagcg ctcgtatccc gcactcagag
      241 ggactccgtc caggcgcttc tgtgcggcga agaaaccgct tcgacgggtc cgatgatccc
      301 ggagcggagc gcgttgctgc cgtcggccgg ttttgcgatg cttcgatcat ccgccatgac
      361 cgttgccgtg cggttcggcc ggcacggcgg cggccacggt caccccgaca aactgaacat
      421 cgttacgttc gccggcggcc gcgtgttcgg tctcgatccg ggatcgatca actacggcgt
      481 cccgctgcat cgcgagtggt accgcagcac catcgcgcac aacacggtcg gcgcggacgg
      541 ccagatccag gcttccgccg acgggcgcca cgaggcatgg gagaccggcg gggggatcac
      601 acggttcacg gcgatcgcgg acaaggtgta ccccggggtg actctgcgcc ggtcgctcgc
      661 gctgtacgga agccggctcg acgatcgttt cgaatgcctg gccggcgcgg agcacgacta
      721 tgactgggca ttccacgcgc cgggacagct ccgcgtgtcg gtgaaattga gcgagcgccg
      781 tggcgctctc ggcgctgaaa acggctacca gcacatccgc ggcgtctcgg agggacggcc
      841 ggacggagat tggactgcgc gttgggaatc cggagatgcg gtgctgacgc tgcgatttcg
      901 cactgcctcc ggcactatgg ttttcacagg cgccgctccg ggccggaacc ctgcggagct
      961 ggttccgatg ctggttgtcc gccggcgggc cgccaacacc gttttcgagg tgactcacga
     1021 gatctcggcg cgctcgtagc cggtccgcct aatctccgct tttcttcgcc gcgtgcgaga
     1081 gggaatagcg cgccggcagc agggaaaagt actcgacgaa cctgttcttc gactcggttc
     1141 cgatttgcct cttcaccagg ataccttcgg ccatgttccg gaaatggcgg ccaagctcag
     1201 tcgacgggtc gacgcacttc cccgccgcca gagcatggtg agcgccggca tagtcgttcg
     1261 ggaagatcag attgacgctg tggcccaaca gttcttcgac ttcctccggt ccgatgatcg
     1321 cccgcttgtg tgttcggttc agcaccagcg aaactctctc ctcgagttcc agcgttcgca
     1381 ggtaactgag cttctcccgt gcgagatgca acgacggcag ttcctgggtg cataccagaa
     1441 agatgcgctt tgactcgtgc atcaggtcaa tcgagtactt ctccatgttg cccgacaggt
     1501 cgacgcagat cgctttgtag tgccgccggg cgaagtccat cagcgcgcgg ctctgcaccg
     1561 gctcgatgcg gaaccccgga ttcagtttcc cagacgtcag cacgtccagc cgcccaaagg
     1621 acgagaccac ttgccgccag agactttcgt ccagattcgc cgagctttcc gcggcgtgca
     1681 ccagcgagta gggggaatcc agcttcagca tgaagccgat cagcccggag ttcaggtcga
     1741 agtccgcgat caggaccctg ccatcctgga ctgtcgccat cgcggcactt acgttgagcg
     1801 ccaccgtcga cgtgcccacg ccgggtttcg acggcagaaa cgtgaacacg aggtcggtcg
     1861 actccaccgg cggcggcata cgctcagcga tctccctgac ccgcgccaac gcctcgcgca
     1921 acgccggcgt cgagaacggc gggtacagga actcgcggac tcccgtgcgc atcaactcga
     1981 gaagcactgc cggctcgcaa ctcgtgtgca acgccaccgt ctgcaagccc gggccatact
     2041 tctcaaggtg ccgtgccgcc gtcatcgctt ccgacagaga gccggcgctc aggaacacaa
     2101 cttgtggagc gaatgcgcga atcgcccgcg acagctccag atcggtcgga tagcgttcca
     2161 gctttcgaac gacgtttatg acacccagcg cggccagagc agcccggagt tgttcttcca
     2221 gtcccggctg agggcagatc agtattgccc gcagaatagt gaccctccag cctcagccta
     2281 gggtgttccg gccgcgacgc caatcaaggg attcacccag acacggttca taaacacctt
     2341 aggctcgggg gatactacct cacagcacca cgcacggatg atacagattc ggcaaccgga
     2401 cctcgccgat cgccacaagt tcgccctcct gcgtcaccgc ctttacgtac ttcgttactg
     2461 tgcgtgtccg gaacggcgac acccgaaagt cccttccctg ccggatctgc ccggctgtca
     2521 gcctgtccac aatctcgcac ggaaactccg gcaacagttg cgccgctggg accagcgctt
     2581 cgccgagccg cttttccgct gacagcgccg ccagttcgtc gattgttcgc gcatgctgaa
     2641 gcgagaattc gcccgacacc gtccgccgca agccctccag aaatgcaccg cacccgagaa
     2701 tttcccccag atcatgagcc atgctccgca tgtaggtccc ggccgagcac tccactacca
     2761 ccctcgcccg gcttccgtcg actccggcaa gctcgatgtt ccggatggtt acttccaccg
     2821 gcttcagatt aaccggcaag tttttgcgcg ccagttggta cgccggtgtt ccgccgattt
     2881 tcttcgccga gatcggtggc ggcgtctggt ggatgaggcc gcgaaaggag ttgagcgcct
     2941 cctccagccg cacgcgattg atctccggtt cggtttttgg ggaagtcggc ttcccgtcgc
     3001 gatcgtaggt gtccgtcgag tagccgaaac ggatgacggc gtcgtaggtc ttatcgccac
     3061 gcatgtagaa ctgagcaagc cgcgtcgccc ggccgatcac gagcggcagc actccggtgg
     3121 ccatcgggtc cagcgtgcca agatgcccaa ccctacgcgt gtccgccagc cggcgcattt
     3181 tgttcaccgc gtcgtgcgaa gtccagcctt ccggtttgtc tgcgacgatc actccgtcca
     3241 tcagcctcaa ctgtagcatt gcgctttctc cgcgaagccc ggcggcccat gctgtacact
     3301 ggacagagtg gaagaggagg tactgggtaa ggcttacgac ggacgcctga tgcggcggct
     3361 ggtcaagtat atgcgcccct ataagggcgt tgtcttcctg tcacttctct tcctgctcct
     3421 gcactccgtc cttcagatcc tcggtcccct gttgaccaag gtcgccgtcg accgttacct
     3481 cgcacccact ccgggcagcc tgccggcgcc cttcgagagc tatctctccg ccgacccctg
     3541 gaccgggatc actcagatct ccgtcctcta ccttctggtc gtcctcggca cgctggtcgc
     3601 tcagtttctg gaaaccgttc tgatgcagtg gaccggccag tgcgcgatgt tcgatctccg
     3661 ccgcgagttg atgaccaggc tccagcagct cgacatcgcc tactttgacc gcaaccccgt
     3721 cggccgcctt gtcacccgcg tcaccaccga cgtggacgtt ctcaacgagc tgttcgcctc
     3781 cgggttggtc accattctcg gcgacctgtt gatgctcagc tttgtcgccg tcgccatgtt
     3841 caagctgagc ccggcgttga cgctcgtgct gctcggggtc atgccgcttg tggtgctggt
     3901 cacggccgtt ttccgccgca ccattacgaa ggcgtaccgg caggttcgta tcgccattgc
     3961 cagaatcaac gcctacctcc aggagcacgt caacggcatc tccgtattac agctcttcaa
     4021 tcgcgagaag aagagcggcg aagagttcga gagcatcaac cggactcaca tggacgcctt
     4081 caaggacgcc atcctcgccc atggatggtt ctacccggtc gttgagttcc tggggatgtt
     4141 ggcgctggcc atgctgctcg cctactcggg ctacaaagtg gaatccggcg aactgaccct
     4201 cggcatcatg gtcgccttct tccaatacgg attgcgcttc tttcggccaa tccaagattt
     4261 gagcgaaaag tacaacatcc tccaatcggc gatggccgct tccgagcgcg tgttcaagct
     4321 gcttgatacg cccgtccgga tcgctgttcc gccggcgcca aagccgttcc ccaccggcca
     4381 ggtgcgcatt gagttcgatg gagtctggtt cgcctacaag gaggaggact gggtactgcg
     4441 cgacgtaagc ttcaccgtcg agcccggcga gactgtagcc gtcgtcgggc acaccggagc
     4501 cgggaagacg acgctgatca acttgctgct gaggttctat gatgtgcagc gtggggccat
     4561 ccgcattggc ggcgtcgatg tccgggaatg cgatccccat gacctccgcc accacttcgg
     4621 cttcgttttg caggacccct atctgttcag cggaaccatc gaggacaata tccggctcgg
     4681 cacgggcggc atcacacgcg acgatcttga ggaagcggcc gaacaggtca acctgatcga
     4741 gtttatccga tcgctccccg acggattcac acagccggtc cgcgagcgcg gctccggcct
     4801 ctccaccggc cagaagcagc tcatcaattt cgcccgcgca ctcgctcaca atccgcagtg
     4861 cctggtgctt gacgaagcga cttccagcgt cgataccgaa acggagatca aagtgcgcga
     4921 agccctctcc agaatggtgg agggccgcac ttcaatcatc attgcccacc gcctgtccac
     4981 catccagcgt gccgaccgca tcctcgtgat gcacaagggc cgcctccgtg agaccggcac
     5041 gcaccagcag cttcttgcgc tcaaggggat ctactggcac ctctaccagc ttcagtacaa
     5101 ggatcaggag atccgcgagc cgaaagcggt ctagcccagg gtctagcccg gattccgtcg
     5161 ttcgagcggc tgggccttac gccgcgtcca aacccggcgc ttcgggccgg agagtgacct
     5221 gatggaccgc tcacacgccg ttgccccgcg cggctatagc gccctgctgc cggcatcgaa
     5281 tgcccgctcg aggaacgccg ccgccagaca tgtctccata tctgtgatca ggtcgagggt
     5341 gcgccggtcc gatgtcgccc accggatcgt cgccaccgaa ggccgttcgg cgatttgcag
     5401 gaggtcatcc tgcggttgtt cgccagccgc cctgcatacg tcctccagcg ccgcttgcgc
     5461 catctccaga gcgtgctggt ccgcctccag attgataccg ctcaccatca ccagcgttga
     5521 cagtaattgg tctttattga tgaagcttgc catcgcccag gacaggtgct ccgaggtcca
     5581 ttgcagttcc acctcgccaa cgcctttcat cggcagcttc agattaaagt tgccgccgtg
     5641 gctgtctagc actgacatca ggtacgtgac cgcgttccgt gccagcttgc tcgaatcgaa
     5701 attctgcttg ctcgcgcagt gaccgaaggc tgaaattgtg aacccatgta tggtcatcgt
     5761 ctctccggcc gccggatgcc tcggcgcggc ccggcggttg cacgtcccgc gctattgtac
     5821 cgctttaccg ccgccgccgt tcccccgtaa gatgactatg gatgcctttc tgttctatcc
     5881 tcgaggccat tgaggaaatc cgcgccggcc gcatgcttgt ggtcgtcgat gatgaagacc
     5941 gcgaaaacga gggtgatctg accatcgccg cgggcatggt taccccagag atcatcaact
     6001 tcatggcgac ctggggccgc ggtctgatct gcctcgccct cgacgccgag cgctccgatt
     6061 tcctccagtt gccgcccatc tcgccccgaa acacctcccg cttcggtaca gccttctgcg
     6121 agtcgatcga tgctcgcgag ggcgttacca ccggcatctc cgcagccgac cgcgccctca
     6181 ccatccgtgt cgccgtcgac ccggccacca agccgcgaga tctggcccgt ccgggacacg
     6241 tctttccgct gcgcgcccgc gaggggggcg ttctcgttcg cgccggccag accgaagcag
     6301 ccgtcgacct cgcgcgcctc gccggcctca ctcccgccgg cgtgatctgc gaaatcatga
     6361 acgacgacgg cagcatggcc cgagtgcccc agctcgagga attctgccgc cgccacgggc
     6421 tgaagatgat ttcggtggcc aacctcattc gttaccgcct cgagaccgag cgcctcgtgc
     6481 ggcgtgtggc ggagggctgc ctggagacgg agttcggccc attccgcacg attgcttacg
     6541 cgagttccat cgaccccgaa atgcacctgg cgctcgtgca tggtgatgtc gccggcacag
     6601 aacgggtgct tgtccgaatg caagcccgtt gcgtctacgg cgacgttttc ggctcgaccc
     6661 agtgcgactg tcaccggctc gtgcacacct cactcgcccg catcgccagg gaagagggtg
     6721 gcgttctcgt ctatctgcat cacacaggcc tcggaatccg gatcgacaag gacccggacg
     6781 gccgcggccg cctctcctgc cacgaccgcg acttcatgca ctacaagacc gcggacggcc
     6841 agcgccagct ccagtatgag agcggcctcg gcgcacagat tctgtccgac ctcggcctgc
     6901 acaccatccg cctgctgacc aaccacccgc gcaagatcgt tggactggaa gctttcggca
     6961 ttgagctggt cgagcaggtc ccagtggccc tataataaag aaactagcct cgccgggcga
     7021 cgtatgtgac taaggaggga gcgctcatgc gttcttctac cggaagcgcc ctgattctgt
     7081 tgctcgccgt ggcaatcgtc ccggcagccg acactaacct gatgaacctt gtcatgccgg
     7141 acgcgcggat gatttccggc ttcaacatcg agcgcgccaa ggcatcgccg ttcgggcagt
     7201 tcctcctgtc gcagatggct gcgtccgacc cggacttcga aaagtttgtc gccgcgaccg
     7261 gctttgatcc ccgccgggat ttgcacgaga tgctattcgc ctccgccggc acgcccgata
     7321 gtgctcacgc catcgtcctc gtccggggct tgttcgactc accgaggatc gaggcgatgg
     7381 ccaaactgaa cggcgccatt atccgggcct acaagggtgt ggcggtcatg cacgggaccc
     7441 agcgcggcgg tccgtggtcg ttcaacatgg gcgccacgaa agccgacggg gcattcgcgt
     7501 ttctcgacaa caccaccgcc gtactcggcg acgctcggag tctccattcc gtcatcgatc
     7561 gccgcgtcgg cggccggggc ctgaatccga gactcgccgc ccgcgcccag cagttgagcg
     7621 aacagtacga catctggggc gtctccatga tgcctgtgtc cgagtgggcc tccaaggttc
     7681 ccgatgagaa cgtgagcgga gttatgcagg gcgacgcgct ccggtccatc gaacagtcaa
     7741 gcggtggcat ccgttttggc aaggtaattg aaatctcggg tgaggcgatc acccgctccg
     7801 aaaaggacgc gaccgcgctc gccgacgtca tccgcttcct cactggcctg cttcagttga
     7861 atcagaagga cgccaggatc acgggacctg caaagctcct ccagtcgctc gacctccgca
     7921 cagaaggtaa cgtaatgaag ctgtcgttgc tcgtgccgga agccgaactc gagaaattca
     7981 tcctcgaagc gaaagcccag gcgcaaaaga ccgccgccgc ccgcaaaaca cgggctccat
     8041 ccgggacact agccccatcc gcgacacagc cgtcatccgg aatgcagcca ccaccgcggc
     8101 gccccgcccc tgccgcccag tccggcggcc tcaccatcca ctcctcagaa ggcacaatcc
     8161 agatcaagta gcactcccac gttggtttta ggagccacgg cggtcaggga gcggtcccta
     8221 cacgcctcaa gctacgatgg aacggtgaaa ccgcggacaa cccacttcct cgtccttctc
     8281 tcgttcctac ttcttccggc cgtctctccc accgtcgcac aaccgaaggc tcgtcacgcc
     8341 ttcgctctcg gcaaagacga cttcctgctc gatggcaggc cgttccaaat ccggggcggc
     8401 gaaatccagc cgaaccgcgt accgtgcgaa tactggcgtc accggatccg catggccaag
     8461 gcgatggggc tcaacaccat cgcaagctac gtcttctgga gctatcacga ggtcgaagag
     8521 ggccgcttcg atttcaagac ctggaaccgc gacatcggcg agttcttccg catcgctcag
     8581 gaggaaggaa tgtgggtttt cctgcgtccc ggcccgtact gttgtgctga gtacgacttc
     8641 ggcgccatcc ctacctacct gttacgcgac cccgagctca agatccgctg catgtatccg
     8701 cgttacactc aagccgccga gcgctacctg cgcgcccttg ccgccgtcgt caagccgtat
     8761 caaatcaccg aggggggccc catcctcatg atccagcttg aaaacgagta cggcagttac
     8821 gccaacgacc gcatctacat gaagtggctc catgaccttt ggcgcaaact tggcatcacc
     8881 gtgccgttcg ctaccggcga tggagccact ccgcacatgc tggaggccgg atcatggccc
     8941 ggcgccgccg tcggactcga ccccggctcg cagctttcac actgggatct cgcccggcgg
     9001 atgaatcccg gcgttcccat attcagcagt gagacctatc ccggctggct aactcattgg
     9061 ggcgagaagt gggcgcatgt ctccgtcgag agcatctcga aggaagtgcg tttcctcatg
     9121 gacaacggga aatcgttcag tctttacgtc gtccacggcg ggacgagctt cggattcata
     9181 gccggcgcca acgccgaccc gttacccgag tcgcagaacg gcatcgcctc cagtttcatg
     9241 cctgacgtca ccagctacga ctatgactcg ccgatcaccg agcaggggcg cccgactccc
     9301 aagtactttg ctctgcgtga cctcatcgct tcgtatttga aaggagagaa gctcgcggag
     9361 gtggcgaagc ccatacccgc cttgtcgata cctcccatcc gcctggagcg tttcaccacc
     9421 atctgggaca atctgccgtc tcccgtaaaa cttgtccagc cgaaaccgtt cgaggccctc
     9481 ggccaatatc agggtctgat gctctaccgg acaaagctca ccggccacaa gagcggacgc
     9541 cttctcatgc gcaatctcca cgactacgcg ctcgtcttcg tggacggcat tctcatcggc
     9601 tcggtggatc gccgactcaa ccaaaactcg atcatgcttc ccaagaccga atcgaaggag
     9661 ccggtcctgg agatccttgt cgaggcgatg ggccacatca acttcggcga attcatcatc
     9721 gaccgcaaag gcatcaccga ccgcgtcctg ctgaacaaca tgacgctcat gaactgggag
     9781 acgttcggct tcccactcga ggagcagtgg gttcagtccc tgaagcgaac gaccaccccg
     9841 gccgcccgcc ccggagtctt cctcaagggg cactttcgcc tcgacaagcc ggctgataca
     9901 ttcctcgaca tgacgggcta tcgaaagggc tatgtctggg tcaacggcaa caatctcggc
     9961 cgctactggg aaatcggccc gcagaagcgg ctttactgtc cggcgtgctg gctcaagccc
    10021 ggcgacaacg aaatcgttgt cctcgacctc caccaggccg agcccaaacc gatcgccggc
    10081 cacgaaaagc ttgacgactg agagagaaga ccgtgcaaca catcgcactg cgccacaccg
    10141 accttaccgt gtcccgcgtc tgtctcggga ccatgacctt cggaggccaa actgacatag
    10201 acgaagccgc ccgtctcgtg gaccattgcc tcgactccgg cgtcaatttc ttcgacaccg
    10261 ccaacatcta caacaagggc gcatccgaga ccattctcgg ccggatcctc cgaggccgcc
    10321 gctcaggcgt cgtcctcgcc agcaaggtgc gtggcaaaat gggagacggc cctaacgaga
    10381 gcggtctctc ccgcgttgcc atcatgcgcg ccctcgatga cacgttacgc cgcctcggga
    10441 cggactatct cgacctctac taccttcacc aacccgacta cgacgtcccg ctcgaagagt
    10501 ctctcgatgc gatcgaccgc ctcgtccgtt tgggcaaggt ccgctggccc gccagttcca
    10561 attacgcaag ctggcaggtg tgccgcatgc tctggctagc agagacgcac agctacaagc
    10621 cggcctgtgt cacgcaaccg atgtacaacg tactcgcccg cggcatcgag caggagtacc
    10681 tcccgatggc gaaggagttc ggcgtctcga cggtcgtcta caacccgctc gccggcggcc
    10741 tcctgaccgg caagcagcag cgcgaagcgc ccatccccgg cacccgcttc gacaacaacc
    10801 agctttacct ggaccgctac tggcacccgg cctacttcga cgccgtcgac cgcctgggcg
    10861 ccatcgccgc gtcggttggc cgctcgctcg tcagccttgc cctcaactgg cttctgcacc
    10921 attcgcccgc ggactgcctc attctcggag cctcccgcct cgatcaaata tctgaaaacc
    10981 tgcgcgccgc cgaggaaggc ccactcccgg aagacgccgt cgccgcttgc gaccaggtct
    11041 gggcggatct gcgtggcatc acaccgaagt acaatcggtg attcgaggag acccactcat
    11101 gccttcatcc cttcgcggac tcgcggtcct attctcgctc tgcgctttgg cgcccgcgca
    11161 aaacacgctt ccactcttcc aagagggcag gatccgcacc ctcatttttt cgggccgcaa
    11221 taaccacgac tggcgcacca ccacgccggc cctgaagaag tacctgctcg acaccggccg
    11281 cttcgacgtt cgcgtcgtcg aggagcccgc gggcacaacc gcagaaaccc tggccgccta
    11341 tcaggtgatc gtgctcgact acaacgggcc ccgctggggg gccggcaccg aaaaagcggt
    11401 cgaggacttc gtcaagtccg gcgggggcct cgttgctgtc catggcgcat cgtgggcgtt
    11461 ttcgggcctg cccgtactcg gcgaccgcca cgtccgcatc aatctcgttg agcctgcttg
    11521 ggccgaatac gcacgcatgg tcggcggcat ctggtctgaa caagagccga agaccggcca
    11581 tggcctgctg cactccttta aggtaaagtt catcgacccg agccacccca tcacccaagg
    11641 tatgggcgaa tcgttcctcg ccaccgacga gctttatcac aacatgcgaa tgcagcccgg
    11701 cgccaaaatc ctcgcgacgg cctatgacga tccgaagatg aaaggcaccg gcaaggacga
    11761 accgatcctc tggaccgtgg actatggcaa gggacgcgtc ttccatactg cgctcggtca
    11821 cgacctcgcc tccatgatgg agcccggttt tgtcacgacg ttcgtccgcg gcacggagtg
    11881 ggctgccacg ggagccgtca ccttgccggc ggcgatcgat cccgccagac ctgcggcgga
    11941 ccgtctgcgg gtgctcgtcg tcaccggcgg gcattctttc gacacgtcgt tctataccct
    12001 gttcgagggt taccccgaca tcgcctggga tcattcggtg accagccacg aagccttccg
    12061 cgaagacatt cgcccgaagt atgacgtcgt tgtcttctat aacatgcacg acaagccgga
    12121 ggacatcacc gaggctgaaa agaagaacct cgtcgatttc gcccaaagcg ggaaaggtct
    12181 cgttgtgatt caccacgcca tcgccaacta ccccgcctgg gactggtggt cgaaggagct
    12241 cgtgcgcggc aagtatctcc tgaagcccga gggtggcatg cccggttcca cttacaagca
    12301 cgacgtcgag atgttcgtcg agcctgtggg tcagcacccg gtgatcgccg gcgcttcgcg
    12361 gatgcacatt tgggacgaga cgtacaaagg catgtggatc gcgccggacg tccaggtgtt
    12421 gctcaggacg ggcgagccga ccagcgacgg tcccgtcgcg tggatcagtc cgtactcgaa
    12481 atcgcaagtc gtctacattc aactcggaca cgatcggctc gcccacatcc atcctggcta
    12541 ccgccgggtc attaaaaacg caattgactg ggcgggtggt aggaaatgag gagcgatttc
    12601 acccgacgcg acatgctacg gctcgccggc gccggtgtgc tgggtgacct cgcattgccg
    12661 gcacagacgc ggcggccgaa tcttctcttc atcctcgccg acgacctcgg ctggggagac
    12721 gtcggcttca acggccgcaa ggaatggccc actcccaacc tcgacaggct tgcctctcag
    12781 ggcaccacct tcacccgctg gtacagcgcc gccaccgtct gcgcgccctc ccgagcttgc
    12841 ctgctgaccg gcaagtacac cattcacaac ggagtgaaac ggaacggaga cgatctgccg
    12901 gcctccgagg ttactttcgc cgaggcgctg aagccgcaag gctacaccag cgcgctgatc
    12961 ggcaagtggc accacggagc actccgcgac ggctcgttcc tccatccgct cgaccaggga
    13021 ttcgactcga ctttcggatt cctcgacgcg cggcatgcct gggaacactt cccgaagacg
    13081 ctcttccgcg gacgtcagga agttccggtc aaggggtaca ccgccgacct cttctccgat
    13141 gaggcggtca agttcatcgg cgaaaatcgt tcacgcccct tttgcctcta cctcgcctac
    13201 atcgagtcgc acttccacgt ggaggcgccc gaggacgaag tggccaaatt tcgcggaaaa
    13261 ttccccgaaa aggaccaaag aacgccccga aacgccgctt acgcggccat gattcaccgc
    13321 atggacaccg gtatcgggcg agtgatgcgc gcactcgagg agaatggcct cgcccagaac
    13381 acgctggtcg ttttcaccag cgacaacggg gccaccttcg agagcggcaa caacggcgcg
    13441 tcgaacttcc acgatagtaa ccggccggtc cgcggccaga agcgcagcct cgaggaaggc
    13501 ggcatccgtg agcccgcctt cgtgtggtgg cccggccgca ttccggccgg ccggaagttc
    13561 gaggaaccgg tgcattccac cgatctcatg ccaaccctcg tcaacgccgg cggcggaacg
    13621 atcgaccctg cctggaaggt cgacggcatc aacctcctcg atgtttggac cggaaaggcg
    13681 caccttcccg agcgcaccct gttctgggag tggaccagcg agggctacaa catgcacgcc
    13741 gccatgcgcg gcgatttcaa gctcctgcgc atcaacggcg ctgacttcct ctacaacatc
    13801 cgcgaggacc cgcaggaacg ccgcaccatc gccgccgcac accccgagat gttccgccga
    13861 ctccgcgccg aactcgaagc ctggaaagca agcgaagtcc cccgcgcccg ttgagaatcc
    13921 ggaaagccta caattccccg tccggcacag tacccgactg aaagcaccgc acttgctccg
    13981 gcgcaaacgg cggttcaaaa agctcaggcc tctcgtggta cgcccggaat aggtgcgcga
    14041 tgtaatgcat tcgctgatgg agttcagacc agtcctttgc gccacaatcg tcgggcgtcc
    14101 cagccgaggg ctcgaatttc tcaagcaggc tcatcagttc gggattttct acgtctcgca
    14161 gcacctccgg gatgggctca ttcaagtgct tcccgagggc tagcaccgtg cccggaatcg
    14221 ccaggaccat tagcgaatcc gtgaccacct gacggcttaa ctgcaccgcg aatcgctcta
    14281 cgctccgccc tagccgttcc aacaccgcaa ctacgggccg gcggaacatt tctcgccacc
    14341 ccgccgcgcc cggaaacaac gcctccacga cgcgcttgcc gaggtcttgc gctgtcacca
    14401 gcgttgcttc gagcgcttcc tggatctccg gttgaagccg cgtctgttcg tgcaggccga
    14461 tctccaggtt cgccagcaac acaaattcgg tcttgacctt tggcgaagct gccgtgcgct
    14521 gctgctggta gtggaagaac gcctgccgca acagatcctg cccttccggc ggcggaccgg
    14581 gccggagccc cttgtggaag ttctgaagct cttcggactc cggggccacg ttaggtgcac
    14641 aaacatgcac aaaatgtgca aattcgcgcc caatttcgtc aaaaaccttg cagtttccgc
    14701 gcgaaaccgc gctgctcgcg cgctcaaacg cgtcgaacgg gctgtgaatc tcctgaacca
    14761 ccttcccaag ccgcgtggcg ggatcgagta gcccccgccg cagcagaacg cgccacagcg
    14821 actgcaccgg atgcaggaat ttagccccca acacgagccg gcgctcgaat tccctgtgta
    14881 ggtcctcagc acggatcgtc cgaccagcct gccgtgacgc ccacgtggcg aacgtgcacc
    14941 agttcgcaca cggtccggtt cgcgacgaga aaaccctgga gagcctatgg taacagtgcg
    15001 tgatttcgag gttacgaatc acagagtccg caatcctcgt gatgcgatcg gcttcctcga
    15061 caccgggagc cgaaatgtag ccgggggttg cgctcatgga tcgttcttcc ttaacgaatc
    15121 ccagtctgtc catcataccc gagggtctca ccggcgccgg agtgatagca tttccgtttc
    15181 ggatgaaggg aggacagaat gcgcatcgaa acgcgccttc ttgcgacgct gctcggagga
    15241 gccatcgcga ttcacgcggc cggtatcgtc ttcgagggaa gcaaaggtcc cggcaaggga
    15301 aagcacatcg tgctcgtcag cggcgacgag gagtaccgct ccgaagaggc cctgcctcag
    15361 ctcgccaaga tcctttcgac gcggcatggc ttccgatgca ccgtgctgtt cgccatcgac
    15421 ccgtcggatg gcacgatcaa tcccaaccgc aacgacaaca tccccggcct cgaaaccctc
    15481 gccgcggccg acctgatggt aatcttcacc cgattccggg atctgccgga cgtccagatg
    15541 aagcacattg tcgactacgt cgagtcaggc cggccgatcg tggctctgag aacctccacc
    15601 cacgccttcg acctgaaaac gagcaaaacg taccagcagt atggctggaa cagcaaggag
    15661 tgggacggag gcttcggacg ccaagtgctc ggcgaaacct ggatccggca tcacggccag
    15721 cacggcaaac agagcacgcg cggcatcatc gcgaagggcc aggagaacca tccgattctt
    15781 cgcggcatcc gcgacggcga catctggggt ccgacggatg tctacgccgt ccgcctgccg
    15841 ctccccggtg acagcacgcc gctcgtcctg ggccaggtgc tcgaaggaat gcgaccggat
    15901 gacccgcccg tcgccgggcc gcagaacaac ccgatgatgc ccgtcgcctg gaccaagacg
    15961 tacacgagcg cccgtggaaa gaaagcccgc gtattgacga ctaccatggg ggcctcgcag
    16021 gatctgctca gcgaaggcat gcgccgcctg ctcgttaatg cctgttactg ggcggccggg
    16081 ttggagaaag aaatcccggc gaaatcaaac gtcgatctcg ccggggaatt cagcccgctt
    16141 cctttccagt tcggagggtt ccggcgcggc gtcatgccgc gcgatctgga aacgcgatga
    16201 atcgaccgtg agtcaagact aacccggtgg cacgaatcct gtgagcttgt gtttgttctc
    16261 ccggatcgct gtaccgaacg aacggcccat gactgtaaca ccgctcgtca ccgtgccgct
    16321 taccttgact cgtgctccct tggagggaac gccacgattc gagatcacta tgatcttgcc
    16381 ggttccgtcg tcgatctgat acccaccggc cgtgaacgcg ccgaatgagt gggtcacgtt
    16441 cccttccacc cgcacgtttc ggttgtgata gtgatacgga tccgagagaa ctcgcccgat
    16501 cttgaccgtt ccgcagcccg tcaggaacag tgctaccccg gccgccaccg ccaatgtcca
    16561 ttgcgatcgt cgccgcatcg tctcctccaa cacctcttga gacgcacccc acaccgattc
    16621 gggttgcagc gaagaatggc tccggcgacg gttaaggttt cgcttcgtcc ggtttcttga
    16681 taatcagcga gaaaacacct gttcccagcc atcctccgat catcgcgacg acgaccgcgc
    16741 tgatcgggtt catcaggacg gtgcgcggca cgagactcac cagccccagg ctcgccgccc
    16801 aatataggaa gaagcccgtc agcattccga cgactagacg gatgggcacc ttcggatcgc
    16861 gccaccagcc cttccggctg cgctgtctcg gaagcgcggc gaggaaaccg ccggcaagcc
    16921 ctcccagcgc tgacagcagc atcagcacga ccggcagcgt gacagtcact gcgccggcct
    16981 gcgaatcgag cccggcggcc gaaacagcga tcgccgtcgc tccgcgccat actggacgaa
    17041 attccgtttg cgcgctcgat ttgccgcgag gaattgagat cacggtttgt gccagcgatc
    17101 ccctgccctg ctcaatcctc agagtaaccg gcgtgtccgt ggcgcttgcg gccgggcgcc
    17161 ggtcgtcgtc gagcagttcg acgatcacgc ttgcagtttc ggccagcgtg atcgccggtg
    17221 gactcacgga cacacggagc tgcgtcacgg gcggcggaac gaaattgaag cggaaaccgg
    17281 atccgccttc aagcgccacc tgcggatctg ctcgcaaaaa cttcacctcg accgggccgg
    17341 cgtgatcgaa cgtcagcttg gcgtggcctt cgtcgccgcc aggcgggatg accagcgggt
    17401 tcgggttgag cgtgccggtt ccgtccgcga agcgcagctt gatttccgac ggcgaacccg
    17461 cccccggccc catgtaaaat gcgctcagcg taaccgggtc cttgccgttc gcccagaagt
    17521 cgcgctgcgg gctgaagctc agggcgacgg gaatcagagg gagttcgccc ggcggtcccg
    17581 gttccgcctg tagcggtgga aggctcggag cgggtttgga aatgggtgcg ccgccgcgga
    17641 ccttcaggct cacgccgccc gccaacaggc ccggactctt cgcccagagg tagacgacgc
    17701 ccccgagagg tagcccgctg agccgccccg acacggagga ctgaccccgg cgaacggtga
    17761 cagtcgtcag ttgaactgca tccttcgggg aggtgcgcaa gctgatctga atcggcgtgt
    17821 ggacggcagt cttcgccggt cggttgttga tgtcgagcag agaaacggtg aaggtgaccg
    17881 gcgcgatccg cgtgaagcgc ttaccctcga ccttgaggtc cagtttcacc gcttggcccg
    17941 actcgcgctg cgcgggcaga gacgcaacca tcgacaccag cagagggagc aggaagaacc
    18001 gtcgttctcg catcgcaacc gtcctcgctt tgagccaacc gcgtttccta gattgtagaa
    18061 cctcgcgccg attacttgcg ggcgcggcgg aaacgagcac gggcgtcggg cgtcattttt
    18121 tcggtggcgt actggtagat gacgcggggt gcgcggtcct tccattcgag caggaacgcc
    18181 tcgaccggct tcggctgctt cttccacgtc tcacggagga accacccgag tccctgctgg
    18241 acgaccttct cggcgtccat catcagcggc ctcagaaact cgaggagcgg ccgcgcactc
    18301 gtcttcgtct tgagcaggcc gagcatcgca accggcacag cgcgccgctg gtacttgtac
    18361 tgcgcttccc gccaggacgc cagcgcgtga agttcggtct gccctccttc gaggagcggg
    18421 gcgaggatct ctccgcacag cacgtcagtg tgcgcccagt tggcgatgcc cgccgcgaac
    18481 catttcgcca gcccgtcgag cgctttgtcg tcgatctcat cgcggaagtg cttcacgaag
    18541 cggatcgcca gcgcgccttc ttcgtacttg ctgtcccgaa acagcatttc gccgagcttc
    18601 agaaagccga ggagtccgat gccgccgtag cgctcgatcc actcgggttc catctcggtc
    18661 cacagggcgt gcttgtttcc catcagtccc caggcgtcgt acccttcggt gaagtaacgg
    18721 gcgtaacggc cggcctgcgc ggcgtcggcg tgggcggcgc agtatttccg gatttcattg
    18781 cgcagagtgg ttaccttgtc catggcggtc cgcgggcatt gtaccaccgc agaactggaa
    18841 atcagttccg ttcggcagga tgagccacgc gtgaaatcgc gttgaccagg cggcggtagg
    18901 gaagtgtttc gagcccgtca aagggaagcc actccccgtc gcgcgtgctc gaatagaacc
    18961 agcgcgcaag cagcgcgagg tgttcctgcc gctcgctccg ggcgagcttt cgcggctcga
    19021 tcgactcggc gatttcgtgc agccgatggt ccatcgaaac ggtcttgccg tctgcgactt
    19081 caaaaccgaa ccggcgcgga ggctgccagc atcctcccag gacgaaccac aactcgacgc
    19141 actccggttc caccgacggc gtcactgcga cgccgttgag ccgcgaaatg tcccgcgcca
    19201 gctcgtcgcg cagtttgagc acctgctgga tcttgtcgac gcgcttatgc tggcgcgctg
    19261 cctcctcgaa attcagttcc ccgctcaggc ggtcgcgtgc atggaccgcg gggtcgagca
    19321 gcgagcggcc gtcggaggag aggaactccg tcactcgctc ggtctcgctt cgatactcgt
    19381 cggcgctgac cacctgctgg cacggccgca ggcacatgct catttcgccg taaatgcagc
    19441 ccgggtgcga cggtgaaggc tccaggtcct cctggcagcg gcgcatctgg aagagatcga
    19501 gaaattggct ctcgaactgc tcggcggagt tccggctgcg gaacgggcca tagtaaagcg
    19561 cgtgggcgcc gctcaaccgg ttggtcacgt ggctgcgggg aaatgggttc gacaggacga
    19621 ttttgacgta cggcggcatc cggagcttca tcagctccag gtaagtctcc gggaagtgga
    19681 ggtgtgccgc ctcgtgaaac accaggttcg tctcgagcca cgacgccgtc aaccggtatt
    19741 cgattcgcgc ggccaccgac cgcaggttca gcagccggga gggatgcgcg cgctcacgga
    19801 gcagccgatg gagacgccgg cggagcaatg cagtgcgagc caggtatggc gatccttccc
    19861 gcggccagac agcgaagacc gcgggctggg cgggcacggt ctcaagaagc gcatcgagac
    19921 cggtcccgcc gtccggaaca cggatcactt ccggcgtcca cagaggcatc cgtcttcagt
    19981 gtagtgcgcc gtgggagagt cgactccgga ccgcggccgc gtacgaagct cgatcagatc
    20041 ccgacctgac ctctcgggcg gttcctgcgg atgttatcta cgtcctgacg gggctcggat
    20101 tcgcattcgg cgacgatgac cgtcactggg ttgtccggcg cagttgcggg aagccccgtg
    20161 aattgtacac ggaactcctc ctgcttgaca cttacgggtc ttccggaggc cagcagcctc
    20221 gccgacttca ccttcgtttg aagcccgccg atcgacacgg tctcacccgg ccagaagtgg
    20281 acatggatgt agagcgtatt gcccttgcgt gtgaagtttg cgaacagaga gtttttcaca
    20341 cggcaccgtt ccgcgtcata gatcgacgta ccgttcttct ccgtccacgc gccgaccgcg
    20401 ttcagaatgc gcaccgactc ttccggaata gacccgtccg gcattggccc aatgttcagc
    20461 aggtaattgc caccgtcgcg cgcgcacgcc accaggttgc gcacgatcgt tttcggactt
    20521 ttccagttat cgtcggcttt gtggtatccc cagctttgat tcatcgtcat gcaggcttcc
    20581 cagtccctgt tctccgcccg tatctcctgc tccggcgtgc cgaagtcgcc agcaagcagg
    20641 ttgcggttgt tgacgatgat atcgggttga agttgcagca ccatgtcgtt catcttctgc
    20701 gactcccagc cttttgcgtc gagtggcacc ggcatgtcgt accagaagac gtcgagtttg
    20761 ccatagtccg tacacaattc tcggatctgt ccgtggatgt aatcgacgaa gcggcggcgg
    20821 gacgcttcat ccgtagcgca tctggcgcca tccgggtggt gccaatccat caatgagtag
    20881 tagaacccgc agcgcaatcc ttcggcccgt gcggcgtcga ggaactcctt cgccaggtcc
    20941 ctgcccgccg cctgtttcgg cgcgctgtag ttggtgagtt tcgtatcgaa gtgacagaag
    21001 ccctcatggt gcttggaggt catcaccatg tacttcattc cagcccgctt ggcgagcttc
    21061 gcccagtcac gggcggcatc cggcttcggt ttgaaacgct tcgcaagctg ctcgtattcc
    21121 gccaccggaa tgccttcctc ctccatcgcc cattcgtggc gtccgagcag gctgtagagg
    21181 ccccagtgga tgaacattcc gaactttgct tcgcgccacc actgtatgcg ccgctcgtga
    21241 tcaggcggcg aggcttcggc agtcaccact gccggttgcg ccggttttgt ttcgttcttc
    21301 tggcatccaa tcgtgcccgt ggacgcggcg gcgaccgcac ccattcccat caattcaagg
    21361 taattccgtc tggacaaaga gaacgttttc atcgcaggat atccttcggc tgtcaagctt
    21421 actgcatttc gccgatccgc acccgttcga gcccttcgtc ggcaatcccg tcagggcctg
    21481 cgtcaaaaag tccggccggg cccgacatag cgcaagtaag ccgcgcccgc cgccaggctg
    21541 agcagcgtca gcgggatgcc tacgcggcag taatcccaga aagaaatgcg tacgtcgtgc
    21601 cgggctcgct gtacaacgat aaagttcgcc acggacccca gcagcgtgag attcccggcc
    21661 agagtggacg acatagccag ggtcagccag cccaggtgcg gatcggatag cctcggtacg
    21721 acggggcgga agacgagtac cgccggaacg ttacttacga tgttggacag cagcgctgag
    21781 aaggcgctca acacccgacg tgatccagcc ggaatcgcgc tgcgaaagcc agcagatccc
    21841 gctcaaggga tgttttttcc accccggcta caacaatgaa caggccggca aacatcgcca
    21901 gcagggacca gtcgatctcg tggtacacct tctccggctt taccctgcgg gtgattagca
    21961 gcagggctcc agcgacgatg gcgaccttgg cgaccggcca gccccagaaa aagaatacga
    22021 tcatgccgac ggaggccacg acggccttcc acatcagtac gcggttcacg cgaaccctca
    22081 gttccgccat ctccacccgt tggcggtccc gaaactccgt ggggaaaaac agcacgatgg
    22141 cggcaaacgt cagtaacaaa cccacaatcg ccaccggcgc cagcgcggct gtgaactgcc
    22201 ggtacgggat gctggacaag ctcccgatca tcatgttttg agggtttccg gtgatggtgg
    22261 cgacgcttcc cacgttggac gccatagcga cagccagaag gtagggtaag ggattgcggc
    22321 gcagcgtggc tgtcacctca agtaccagcg gggtcagaac cagacacatc gtgtcgttga
    22381 cgaaaaacgc cgaaaagaac cccgagacta ggacaatggt agccagcagc gtccacgggt
    22441 ggtgggcgtg ccgcactgcc caggtgctca ccagacggaa aaaccctgac aaccgcaggt
    22501 tggcgaccac gatcatcatg ccaaacaaca acaccagcgt tgaataatcc accgcctgaa
    22561 ttgcctcttc gaacgtcagg acattgcagc cgagcatgag gctggcgccg atgatcgccg
    22621 cacccgtgcg gtcaatccgg aggcctggaa aagtgccgat cgcaaggacc aggtaggtgc
    22681 cacaaaatat tgccagggca atcacaaagg ttcagtctaa cggaatacag gggcttgaca
    22741 ggaaccgctt tcggcttaga atgagtgtaa ttgcggtctt tatgcaaccg aattgcatgc
    22801 acactcgcac cgaactgatt gcgaggcggg acggagtgga ctatgttcgc tgtctggact
    22861 gcgatcaggt cttcgagtcg gatgacctcg agccggtacc cgtcaccggt gaggacgaat
    22921 agggtagacg tcacgttcca ctcctgagat tccctgaaac ggccgggcct gggacgcccg
    22981 ggaccgaagc tcctcgggac cggatgtatc aggccgtggt atgctttcaa aaatccgcgg
    23041 tctcgccagc caggattgcg agtgcggact gcgtgattca gccatgagga agtcggttcc
    23101 acattccggg aaagccgctc caaaaagcag ctcttcaaag gagcaccggc atcgcggcgc
    23161 cgcgcaagga gaggtggcgg aaacgcgaga ccggaaccca gtcgcgtcgc cactgaattc
    23221 caaggaacag gccgcgttgt tcgagaaggc agtcgccctg tttcacgctc gcgagttcgc
    23281 gaaagcgagg gtactgtttg aacgggctgc cgacggtccg gtgatcgaga tggcgcactc
    23341 ggcacgagtg cgcgcgcgaa tctgcgagca gcggctcagc cggacgccgc cagccctgtc
    23401 aaccgccgag gagcactatg actacgggat cgcgctaatc aaccagcgtc agcttgacgc
    23461 cgcccggcga cacctggagc aagccgcgag catgaaccct ggcgcggacc acgtttacta
    23521 cgcgctcgcg ctctgccacg ctctgaaggg agaggcagac gcggtgtacg cgaatctcag
    23581 ccgggccatt gaaattgatc ctcgcaaccg cggacgcgcg ctgcgcgatc cggactttgg
    23641 ggacctcgcc cgccgcgccc cgctatccga tctcctgtac cctgagaagg agcggacggc
    23701 ctgacggacc gcaaccgccc gccggaacac ccgtctcaag aggtgaatgt gtgactcgcc
    23761 agaacgctcc gctacggatt gtcgcgatcg gcggaggcac gggactcgcc acgttgttgc
    23821 gggggctaaa accgtactca cttggatcgg gcacagtccc ttcgcccacg gaagccggcc
    23881 gcgcggtgga aatcacagcg gtggtcaccg tgacggatga cggcggcagt tctggccgcc
    23941 tgcggcgtga gttcgacgtg ccgccgccgg gggatatccg aagctgcatg gttgcccttt
    24001 ccgaggacga agcgcttctt tcgaggctat tccagtaccg ttttcgcacc ggacggggct
    24061 tgaaaggaca tagtttcggg aatctcttct tgaccgcgct gagcgaagtg accggcgact
    24121 tcgctcaggc ggtgaaggtt tcctctgaag tgctggcgat cgccggcgac atccacccct
    24181 cgaccggcgc taacgtcact cttcaggcac gtctcgccga cggaacggtt gtttgcggcg
    24241 agagccgaat cagccgcagc cggtcgccca tcgagtccgt cttcctcgag ccgcgccgct
    24301 gccgcccgct gcccgaaaca tgcgacgcca tcacgcgggc tgacatcatc acccttggac
    24361 ccggatcact ttacaccagc gtgataccaa atctgctggt ggagggcatt ccggaggcga
    24421 tcaagcgctc ccacgcgatc aaggcctatg tcgtcaatct gatgtggcaa cccggcgaga
    24481 cgatcgacta ctccgccgcg gaccacatcg ccgcgatcca cgaacacgcg cgcatgcggt
    24541 tgatcgactg tgtgctcgtg aacacccggc cggtcagccc tgttctgcgc cgccgttacg
    24601 cccgtgaaca cgcgaggccg gtagagtccg acctggcaag gctgcgcgaa atgggcttga
    24661 caatcgtgga gggcgacttt ctggaggaat ccgacaaggt gcggcacaac tccgaagcgc
    24721 tggcagaggc ggtcctgaaa cttgccggag aaagacaatc gcggcgactc acgaagcgtg
    24781 ctgccacgcg gtgagccaca atgaacctat ggatatcccg gtaaccgttc tgatcctcgc
    24841 ggccggcctt ggtacgcgca tgaagtcgaa gaaagccaag gtgctccatc gtgcgggcgg
    24901 gatggcgttg atcgagcacg caactgacgc cgcactgaaa ctgactccac cggagcgcgt
    24961 gttcatcgtg gtcgggcacc aggctgacga ggtgatggcg gcggtgggag accggggcgt
    25021 caggttcgtt cagcaagccg agccaaaggg caccggacat gctgttatgg ctgctcgcga
    25081 ggcgctggag ggcgccggcg ggctcctgct ggtcctctac ggcgacgctc cgctgctcac
    25141 agcggacacg ctcctgcggc tggtggcccg gcagcagcag ggagacgtgg cggccacagt
    25201 actcacgacg atgctggacg atccgtccgg atacgggcgc gtccttcgcg acgagcacgg
    25261 gcgggtgcgc gcgatcgtcg agcagaaagc cgcgtccccc gcgcagctcg ccgtacacga
    25321 gatcaactcc gggatttact gcttcgactc cggcccgctg tggaagcata tcggcgagat
    25381 caagcctgaa aacccggcta acgagtacta cctcacggac attgtcggaa ttctccacgg
    25441 caagggccac gccatcgctc cgctgctccg agaagaccca cgggaactgc ttggcatcaa
    25501 cacacgggtg gagttggcgg cggtggaccg aatcttccgc gaccgaacga tacggcggtt
    25561 gatgctcgac ggggtcacga tcaaaaaacc ggagaccgtg actatcgaca gcggcgtgtc
    25621 aatcggtatg gacacggtga tcgaaccatt cgcgcaggtt ctcggacgca cgacgattgg
    25681 agaggactgc gtgatcggcg cgtgttcgat catccgtggc tcgcaactcg gtgacggcgt
    25741 tgtggtggag ccgttcacgc tggtactgga ttcggccttc gaaccgggtg cccgcgtggg
    25801 tccgttcgcg cgggtacgga tggagagtca cgtggggcgg gaagcgcgcg ttggcaactt
    25861 cgtcgagcta aagaaggccc ggctcggcgc gggggcgaag tcgctgcacc ttgcgtatct
    25921 cggagacacc gagatcgggg aagaggccaa catcggtgcg ggcacgatca cctgcaacta
    25981 tgacggcgtg cggaaacacc ggaccaagat cggccgtggc gtgtttgtgg ggagcaacgc
    26041 cacgctagta gccccggtgg tgatcgggga cggcagctac atcggcgccg gctcggtagt
    26101 gacggaaaac gtccccgagg acgcccttgc cctcggccgc gcccgccagg tgaacaaagc
    26161 cgaatgggcc aagaaacggc gggagcggaa attgcggccc gacacgcagg ggtgattcaa
    26221 gaccgacctc agcaaaccct ctccgagcac gtattcgctg gaacccgccc ttccggcgcg
    26281 gtgtctaccc gaacatgaat ggtctggctc agtttgacag ccgcgtgccg ctgagatgct
    26341 gcctggctgt cgtcgcactt ctattgtccc tgccaccgat gcaggcggcc gaacccaagg
    26401 ctgacgagat cgaccggttg atgcaggcct acacgaatac cgtgggcttt cagggggctg
    26461 tgctggtggc cgacaggggc acagtcatcc tcaaaaaggg ttacggtctg gccaacgtgg
    26521 agcttgacgt ccctaacaca cccgaaacca aataccggct gggatccctg acgaagcagt
    26581 tcaccgcgat gctggtgctg caactcgtgg ccgaagggaa ggttcgtctg gagagcaaac
    26641 tcagcgaata tgttcccgag tatccgaagg ccacgggcga acggatcacc attcaccagt
    26701 tgctgaccca ccagtccggc attcccaatt acataacccc ggagttcctt gccaaacgct
    26761 cgcgcgagtt cttcagtcca acggacctgg ccaaggtgtt ctgggaaaga ccgctggatt
    26821 tcgagccagg cgcgaagtac tcgtatacga actctggcta ccacatgctc ggaatcgtgg
    26881 tcgaaaaggt taccggaaag tcctacgaac aggcgctcag ggagcgcatt ctcgatccgc
    26941 tcgacatgcg ggacaccggc tacgatcaca cgcaagcgat tcttccgcgc cgcgcggccg
    27001 gctacaccaa gacgcccaaa ggcctgcaaa atgcggcctt tggagagatg tcgattccct
    27061 acgcagcggg cgggatgtac tcgacggtcc tggacatgag gaagtgggac gcggcgctgt
    27121 acaccgagaa gctgctcgat aggaagcacc tggatctcat gttccagccg gcagttaaga
    27181 tcggcgcggg ccgggcctac ggatacggtt ggatgctctc agtcctcacg ctcgccggca
    27241 gcgggcagaa gcttcccgtc atagagcact tcggcggcat tatcggcttc accagcatga
    27301 tagtgcggct gcccgaggag cgtagcctga ttgtcgcgct ggcgaatatt ctcggcgtcg
    27361 aatggggcgg tgcaaccaga ggcatcgccc agattctcca tggcgaacaa ccggagacgc
    27421 cgaagaagcc aaccaggtaa agcctgtctc cggcgattag cggcgattgg aggacgtcca
    27481 ccgggcgcgg ccgcccgtct cgccgagaaa cttcatcgcc tcaagatggc agagcaactg
    27541 gatgttgacc agatcgtctg gggtaaaaac gaccagcttg tcctgccgct tcgccgtcga
    27601 aaagccgtgc gacggcgact cgatgactac gctcagcgca ccgcaatgca gattcagcgc
    27661 ggtgtcgagg ttatagggcg agatgttctt cacgcgatcg gggttgcctt ctttcgccgg
    27721 atcctcggtg gcctgatact tgtcgagtgc cagccgtccc tgcacgcggc ggactaactg
    27781 ctcgaatacg ggcaccaagt cgggttcgac gaatggacgg agcatgatcg ggaacacagc
    27841 gcctgtgtgc atgttcatga tgaggtcggg gcgctcgcgt gcggtgagat cgaacagcgc
    27901 ctgggtctct ggctggcgtc ggccgaagaa gtcgtcgtgc tggaaattga cgccagcgtc
    27961 gttgggatag ccgcccggaa actcgacgcg cgaaaaatcg agagggatga actccttgca
    28021 ctgcggccag ccgatcatct tgccgcccgc cagtccgccg gtgttgaagt attcgtggac
    28081 cgtc
//
LOCUS       DATIFQ010000026        15160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_581629, whole genome shotgun sequence.
ACCESSION   DATIFQ010000026 DATIFQ010000000
VERSION     DATIFQ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15160
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581629"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VLE22_01000"
     CDS             complement(<1..538)
                     /locus_tag="VLE22_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13001.1"
                     /translation="MEILRLMCDQIRHRGPDDDGYHVDDGCGIGMRRLSIIDLNTGHQ
                     PMSNEDGSVWVVCNGEIYNFRELRNDLEARGHRFTTQSDTETLVHLYEDAGADAVSRL
                     RGMFAFALWDAKRRRLLLARDRFGKKPLYYAVLPSGLYFASELKCLRAAGVPLGLDPE
                     ALRLYFQFNYVPDPYSPFQ"
     gene            773..2050
                     /locus_tag="VLE22_01005"
     CDS             773..2050
                     /locus_tag="VLE22_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HSB13002.1"
                     /translation="MDTGNRVAVFGLGYVGCVSAACLARLGHHVTGVDPDEFKVRSVL
                     DGRAPFYEPGLEELVRDGAKAGRLQATVSAAEALEDAEVALLCVGTPSERNGNLSVRQ
                     LQKVAAEIATCLHSRTAPLVVAVRSTVFPGTCEEVVMPLLAPFAGVSVLSHPEFLREG
                     NAVRDFLAPSLLVVGGSDKGALEKVAGLYINLKVDACLVSLRTAEMIKYACNAFHALK
                     VSFANEIGALSARCGVSGEEVMDTLCRDVVLNISRAYLKPGFAFGGSCLPKDLRALTF
                     RASRLDLQLPLLQAVLPSNEHQLQRAIQAVLDAPSKRIGFFGLAFKENTDDLRESPVV
                     ALLEQLIGKGRAVRVFDPHITLDQIYGSNQRFLLDAIPHIARLLDARFEDTLGWADHL
                     VITQKPQASYLRLIDDSGLPVLNLMGGDSARSR"
     gene            complement(2051..4015)
                     /locus_tag="VLE22_01010"
     CDS             complement(2051..4015)
                     /locus_tag="VLE22_01010"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HSB13003.1"
                     /translation="MRRPREIAFRVRQEASNLRLAAFPPETTSVEPAPLAGLPNPDAV
                     AEALRHTAFAAEVIALAEMVLAHRFPLFGSVADTGPEIDWRRDCIHGISSGLPYFRLV
                     RYLDFAAVGDHKIVWELNRHQHLVLLAQAWLLTGRREFLAELTAQIDDWIDANPYMRG
                     INWASALEVAFRAVSWLWIYHIAGADLESSFRARFLSALCRHGAYLEANLSVYFSPNT
                     HLLGEAVALHALGLLFPSFPRAGDWARQAAQIVAEQMEAQVREDGSHFEQSSYYHLYA
                     LDMFLFHQILCETDAGYTGKLRRMAEYLAALMGDTGKVPMLGDDDGGRFFHPYGAREH
                     FGRATLATCAVLFDRPEWLADRAAIGEQAAWWLGGESLDNPRTAAPVSSLARLFPDAG
                     VAIIQDEGLHIICDAGPFGSGSGGHSHSDTLAIVVRLGADEILIDPGTCTYVADPALR
                     NCFRGSAAHNTVRVDGVDQGVANGPFSWASKPEVRILGWRSDPNHAWLAAECSFAKIR
                     HRRSLLLLRPFLLIVADQIDGLAGLHRLEQFWHFGEPVTPVAPGTLRIGTSALLSFKG
                     GERSDLSEGAEFGWRSPVFGTKATAPVLCVQRECELPARFWAVLDFSGARDATLSDGP
                     GTGCVYRAGGREISLDYEEGSALAWKLASV"
     gene            4144..4785
                     /locus_tag="VLE22_01015"
     CDS             4144..4785
                     /locus_tag="VLE22_01015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB13004.1"
                     /translation="MVRALLRKLFTELHLYGLYSLYVKGPLRDDGWFRSFDEGRAVDK
                     DGNPVPFITYPAIEFLSRRIHPEMSVFEFGSGSSTLWWASRVREVVACEHDPKWFLEI
                     RARARENVTVIHHPLEYGGAYSKAILNFKDRFDIVVVDGRDRVNCAKNCLGALKLDGV
                     VLWDNSDRTKYEEGYRFLGDRGFRRLDFVGMCPIINERSQTSIFYRRENCLGI"
     gene            complement(4846..5976)
                     /locus_tag="VLE22_01020"
     CDS             complement(4846..5976)
                     /locus_tag="VLE22_01020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB13005.1"
                     /translation="MRRAVLSVFGVEPFRIGGNEAFARELSSQLGRHGWESILCFLSE
                     PPEAVRRFLALSNVSFETLPGSVSLAWQPSADLVRLLRRCRPEILHLHFTGFVSPYPW
                     LARLCGVKRVFFTDQSSWPEGYVPRRAPLWKRAAVRLINAPLNRTLSVSEYGRRCLTS
                     HDLIPPRAIERVYNAVDFSMVGGTGDEFRRKHGIPAERPLVIQVSWIRPEKGIADLIE
                     AARRVIEQEPQVHFAVVGDGSWREEYQRQAEAAGLGGNITWTGLIENPFAEGVYAAAD
                     VVCQLSRWEEVFGWVIAEAMATGRPLVATRVGGIPELVDHGETGFLVNRGDAGEAASS
                     ILTLLRDPDLRERMGCAGRKRAAERFNLTENVGRVVELYGIA"
     gene            complement(5990..7069)
                     /locus_tag="VLE22_01025"
     CDS             complement(5990..7069)
                     /locus_tag="VLE22_01025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB13006.1"
                     /translation="MSFRINWLRKRLHDAVNYRLRTLAGGQFSVRCRPVSIIFLLTEL
                     CNARCVHCDIWKNRGREDTPTADQWKTVLTDLRRWLGPVHVVFSGGEALLKPFTTDLV
                     AHASSLGLFLEILTHGYWDDQSKIERMALARPWRVTVSLDGIGDTHTKIRGRDAFFEK
                     TSTTIQTLLRVRKEKRLPYHVRLKTVVMSHNLDGVCEVARFATQDGMHIFFQPIEQNY
                     NTPEDPRWFETSENWPRDTEKATEVVRQLIELKRGGLHIDNSYEQLEAMIPYFRNPDS
                     LRVSTQFHAAHERRALCAALTTLQFQANGDVTVCNGQKPVGNVKTMPARRIWEGRPRF
                     WEAGCCLDRRCSDAEKQTLSLPVVQ"
     gene            7377..9500
                     /locus_tag="VLE22_01030"
     CDS             7377..9500
                     /locus_tag="VLE22_01030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSB13007.1"
                     /translation="MWRFTDRFDLQMIVQSSRGVARGPVARLVAEGARNTHDWTDAKA
                     SLLKAFDESGITSVFLDPEQGGFIEGPKNLALALVAFELAWVDAGAATGSLAGCLALA
                     PIHERGTAAQRDYYLRLCAPAAPGEERQPWRGAFCLTEPIPFVGVETGLLGGKVRIAE
                     WKEGEEPVLQVDKRGRFITNMGFANFVTAAVDSADPRIKGSCMVILHETDPGVWDRGT
                     PARKLVHQLSSTNDPIFGQRVPAGRIIGGYTVKDGVIVPRYSHGEIIEAVFRRTRVTV
                     ALMTSAKLLSAVEPVIRYQRGRFRGGDIAEPGSPRYELGLQMKEDVLHRLVDVWATGE
                     AGTSLGFEAARLFDQLDPLERKKEEIFAAEGVGRGSAAFKAMRRYQKDALDCLQHGVN
                     CDQPMIQYVVLDSLANVLCPACKLWNTGHGATMMREAVSLMGGYGITEDCPGFLGHKW
                     MDAQLEATYEGPEAVQRRQLSVTMTNELFLAQFHQWIAEMRDIASKRPGTGACTLATA
                     MQLWLWTLTYLQKATDPDGSKLYHSSRQGVTFPLADALCWLLACRYFILDVLELSEKG
                     PANPVVAEGLPGLLKFYTDLCHVQSARAAGEVARVCSELVYGYRPHPAWETPDYAGCF
                     KSDELIAIEAMVPGMSSVATDVVESDGSHPAKEGPCATFDDVEQFIRIRNRLDGCLTG
                     SHLAKERAADALTKVMIPEALDYPA"
     gene            9497..11341
                     /locus_tag="VLE22_01035"
     CDS             9497..11341
                     /locus_tag="VLE22_01035"
                     /inference="COORDINATES: protein motif:HMM:NF045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13008.1"
                     /translation="MSEVTVERQTIEVDIVCVGFGPATGGFLTELSRRIMNPDGTPRL
                     ESRVMPGMPLQVICYERADDIGFGVSGIVTKARGIRASFPDLDHTEIPMAAPVREEKV
                     LFLLDPVGASRRSAALRAADVAVRAIGCEHNAIELPYTPDFLHKDGGLILSIGQFNQW
                     VGQRLMDVGAVQIWPGTPVAQPLIEEDRVIGVRLLDQGVSKAGEPEVGFMPGMDIRAA
                     LTVVGDGPVGPIGRQLTERFGMPEGHHQHEWAVGMKMVIDLPEDTALEPGFVLHTFGY
                     PEPEIFGFLYVHPDRVASVGIFVPSWFDSPVRTSYRYLQHYIQHPYLWRYLKGGRLRS
                     WGAKSLQESGKLGEPHLAGNGYARIGEGSGSTNVLTGSGVDEAWTTGVLLAEGVAELL
                     EARQPFTKENLDRAYVARRRASWVESEGKVAEHARIGFQKGLVTGLLGMALAGLTKGK
                     VVLPGEAVPPYDRLPTFEEYYAGRIPPLEIARIRQECLARGSSVHEALMERCGWPPIP
                     YDGQLLLSQQDALLVGGKVQAPPGYADHVVFLYPSLCETCGTRICIEVCSGEAITASA
                     EGPPQFDREKCVHCGACLWNCSQALEEDPERTNVEFRAGAGGLHSTEN"
     gene            complement(11377..11919)
                     /locus_tag="VLE22_01040"
     CDS             complement(11377..11919)
                     /locus_tag="VLE22_01040"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HSB13009.1"
                     /translation="MKQAIVLRYPLVAAICVATLAGAANQRLDVPFFAQKKNGCGAAS
                     VAMVEHYWSGRPAKQSATAPSPEQVYQQLYQPDRRGILLADMKRYLEDHGYRAVTLRG
                     EWVDLERHLSKGRPVIVGLKPGRSKGLHFAVLTGTEGNHVWLNDPTRKRPNRVDQAKF
                     EKQWEFADRWMLLATPASPE"
     gene            complement(11916..12254)
                     /locus_tag="VLE22_01045"
     CDS             complement(11916..12254)
                     /locus_tag="VLE22_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13010.1"
                     /translation="MRHFPLYQLLSKILVVVLCLSLVAPVGRAAEAASTSPSNADSPA
                     APEPATVTSAPANTGTGSPELLTAEELSELSARAEDPGPEVVGGSLSNEHLTYIVIAL
                     AAAVIVLIAK"
     gene            12742..13293
                     /locus_tag="VLE22_01050"
     CDS             12742..13293
                     /locus_tag="VLE22_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13011.1"
                     /translation="MTVRVYDYSGVPANVQAAARDGVAAIFHRAGVELMWVDCPITAP
                     SNTRPAACDIGPVQGLLTLKIVPESMAGGFRLPRDKFGLAVREYGAYVFFDRLRNHTA
                     AKSLLLPPILGIVTAHELGHLLLEKGHSATGLMAEGLLSTQFRDAEKGVWPAFTLDQI
                     AWIQRQPKPALVAGAGSLPSGMN"
     gene            complement(13309..>15160)
                     /locus_tag="VLE22_01055"
     CDS             complement(13309..>15160)
                     /locus_tag="VLE22_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HSB13012.1"
                     /translation="FPRRGYRFVYPIETQCNGEPEVAAAEAVTTQAAALRRRSWVLFY
                     VGVTLVLALAAAFLAVRHYLTTARKPDTQLRPVPLTTYVGSEYMPSFSPDGNQVAFAW
                     TGPNRDNLDVYVKMIGTENAVRLTTGPGPEGMPAWSPDGRSIAFMKVLPENKVGIFLM
                     PAIGGPDRKVAMINPCSEGLSWHASGEWLAVTEQNPLGAPCSIYAVSITTGEKRKLTN
                     PQAPSADSFAEFSPDGSRLVFFRSRVAGDLYIVTVSDQMVPAGEPRRLTSLNLFRSTA
                     NWTPDGKEVIFSSFQEGEPDELWRMPASGESAPRRVAIQGEGGAFPVISREGNRLAYV
                     HFTWNTDILRLPINGRKGEGSEPTPFAASTRSDLAPQYSPDGKRVAFISVRSGTNEVW
                     VCDADGSNPVQVTTMAATMTGAPRWAPDGQQLVFDSTKQGSFDIYTVRADGGAPKRLT
                     DDPADDGVASWSRDGRWIYFASDRSKDWQIWKMPAAGGKAVQVTQKGGRLAFESADGR
                     DLYYSKDFLGTSIWRMPAGGGEEVQVLESALGFSFVPAAQGLYFVAHDHAAQGSSIRR
                     LDLATGHIATLFHPQRLIDLGLAVSPDERFLLYAQRQDTDTDLMLVEGFR"
BASE COUNT         2796 a         5030 c         4486 g         2848 t
ORIGIN      
        1 cttggaacgg gctataggga tccggcacgt agttgaactg gaaatacagg cggagtgcct
       61 ctgggtcaag ccccagcggc acccccgccg cccggagaca tttcagttcg cttgcgaagt
      121 acagtccgga cggcaggact gcgtaataca gcggtttctt tccaaaacgg tctcgtgcga
      181 gcaacagccg ccgccgtttt gcatcccaca gggcaaatgc gaacatgcct cgtaggcggc
      241 tcaccgcgtc cgcccccgcg tcttcgtaca ggtggaccag cgtctcggtg tcgctctggg
      301 tagtgaaccg gtgacctcgc gcctcaaggt cgttgcgtag ttcgcggaag ttgtaaatct
      361 ctccgttgca aaccacccag acacttccgt cctcgttgga catcggttgg tggccggtat
      421 tcagatcgat gatgctcagg cggcgcatcc cgattccgca gccgtcgtcg acgtgatacc
      481 catcgtcatc gggaccgcgg tgccggatct gatcgcacat caggcggagg atttccactg
      541 ggggctctcc ctcgcgtaca acgaatccgg cgatgccaca catagtaact gggagaacgc
      601 gacagcgccc cgctcctgtt tactgtagca ctcggcagtt gtgaggcagt tgtggttcag
      661 gccatcggct ttgtgtggcc tgccatgctg gtcgacatct cgccgcccca ctccacagtc
      721 atataccgcc cgagtcaccg atgccggaca gggggcgtgg catactagtt cggtggacac
      781 gggcaaccgc gtagcagtat ttggcctcgg ttatgttgga tgcgtctcag cggcatgcct
      841 tgctcgcctc ggacaccacg tcactggcgt cgatccggac gagttcaagg tacgcagcgt
      901 gctcgacggc cgcgcgcctt tttatgagcc gggactcgag gaactcgtgc gcgacggtgc
      961 caaggccggt cgcctccaag cgaccgtgag cgccgccgaa gctctcgagg atgcggaggt
     1021 cgccctcctt tgcgttggca ccccatccga gaggaacggg aaccttagcg tccggcagct
     1081 ccagaaggta gccgccgaga tcgcgacttg tctccattcg aggactgcac cgctcgttgt
     1141 cgcggtccgt agcaccgtct tcccggggac gtgcgaagaa gtggtgatgc ccctccttgc
     1201 gccttttgcc ggagtctctg tgctctcaca cccggagttc ctgcgcgaag gaaacgctgt
     1261 gcgcgatttc ctcgcgccgt cgctccttgt cgtcggcggc tcggataagg gcgcgctcga
     1321 aaaggtcgca ggcctgtaca tcaacctgaa ggtcgacgcc tgtctggtca gcctgcgcac
     1381 cgcggagatg atcaagtatg cctgtaacgc gttccacgct ctgaaggtca gctttgctaa
     1441 cgagatcggc gcgctgagcg ctcgctgcgg cgtctccggc gaagaggtga tggatacgct
     1501 ctgccgcgat gtcgtgctga atatctcgag ggcgtatctg aaacccggct ttgccttcgg
     1561 cggctcctgc cttccaaaag atctgcgggc cctcaccttc cgcgcttcgc gcctcgacct
     1621 ccaactcccg ctcctgcaag cagtgctccc cagtaacgag catcaactcc agcgcgcgat
     1681 ccaggccgta ctcgacgctc cgtccaagcg catcggcttt ttcggactgg cttttaagga
     1741 gaataccgac gacctgcgcg aaagcccggt ggtggcgctg ctggagcaat tgatcggtaa
     1801 gggacgtgcc gtgagggtgt tcgatccgca catcaccctc gatcagatct acggcagcaa
     1861 tcagcggttt ctgctcgacg cgattcccca tatcgcccgg ctgctcgatg cccgcttcga
     1921 agacacgctc ggttgggcgg atcatctggt aatcacgcag aagccccaag cctcgtactt
     1981 gcggctcatc gacgacagcg gccttccggt cctaaacctc atgggtggtg attcggcccg
     2041 gagcaggtag tcagacggac gccagtttcc acgccagcgc gctgccttcc tcgtagtcca
     2101 gggagatctc ccggccgcct gcccggtaca cgcacccggt tcccggtccg tctgaaaggg
     2161 tcgcgtctct ggcgcccgaa aagtccagca cagcccaaaa cctcgccggt agttcgcact
     2221 ctcgctggac gcacagcaca ggcgcggtcg ccttcgttcc aaaaacgggt gaccgccagc
     2281 cgaactcagc gccctcactc aggtcggagc gctcgccgcc cttgaagctg agaagtgcgc
     2341 tcgtcccgat ccgaagcgtc cccggagcaa cgggtgtcac tggctcgccg aaatgccaga
     2401 actgctctaa cctgtgaagt ccagcgagac cgtcaatctg gtccgccaca atcaggagga
     2461 acggccgaag caacaggagg ctgcggcggt gccggatttt cgcaaagctg cactccgcgg
     2521 ccagccaggc gtggttcgga tccgacctcc atccgagaat ccgcacctcg ggcttgctcg
     2581 cccaggaaaa cggcccgttc gcgacgccct gatcaacgcc gtccacgcgc acggtgttgt
     2641 gagcggccga gccgcgaaaa cagtttcgca gagcgggatc ggcgacgtaa gtacaggtgc
     2701 ccggatcgat cagaatctcg tccgcgccaa gacgcactac gatggccagc gtatccgaat
     2761 ggctgtgccc gcccgaaccg gacccgaacg gccccgcatc gcagatgatg tgaagcccct
     2821 catcttggat gattgctaca ccggcgtcgg ggaacaaacg tgccagagaa cttacggggg
     2881 ccgctgtccg aggattgtcc aaagactcgc cccccagcca ccacgcggct tgctccccga
     2941 tggccgcgcg atccgccagc cattcgggcc ggtcgaatag gacggcgcac gtggccagcg
     3001 tcgcccggcc gaaatgctcc ctcgcgccat aaggatgaaa gaaccggcct ccgtcgtcgt
     3061 cgcccagcat cggcactttc ccggtgtcgc ccatcagcgc cgcgaggtac tccgccattc
     3121 gcctcagctt gccggtgtag ccggcgtccg tttcgcacag aatttgatga aacagaaaca
     3181 tgtcgagcgc gtacaggtga tagtaactgg actgctcgaa gtggcttccg tcttcacgca
     3241 cttgcgcttc catctgctct gctacgatct gcgccgcttg tctcgcccag tcgccggccc
     3301 gtggaaacga gggaaacagg aggccgagcg catgcagggc gaccgcctcg ccgagcaggt
     3361 gcgtgttcgg tgagaagtac actgacaggt tcgcctcgag gtaggcgcca tggcggcaaa
     3421 gcgccgatag aaagcgtgcg cggaagctgg actcgagatc agcgcccgcg atgtggtaga
     3481 tccagagcca tgaaaccgcc cggaaggcga cctcgagcgc gctcgcccag ttgatgccgc
     3541 gcatgtacgg attcgcgtcg atccagtcgt cgatttgtgc ggtaagctcc gcgaggaatt
     3601 ccctccttcc ggttagtagc caggcctgcg ccagaaggac aaggtgctgg tgcctgttca
     3661 gctcccaaac gatcttgtga tcgcccacgg cggcaaagtc caggtagcgg accaaccgga
     3721 agtagggcag accgctcgag atgccatgga tgcaatcacg ccgccagtcg atctccgggc
     3781 ccgtatcggc caccgatccg aacaggggga aacgatgcgc cagcaccatc tcggccaacg
     3841 cgatcacctc ggcggcgaac gccgtgtgcc gtagcgcctc ggcgaccgcg tccggattgg
     3901 gcagcccggc gagcggtgcg ggttccaccg aggtggtctc cggcgggaac gccgccaggc
     3961 gcagattcga tgcttcctgc cgaacacgaa atgcgatctc ccgcggacgc cgcacgctaa
     4021 cgccaccccg tcatacgctg atccaacggt gtagacatta gcacacgact tgcacgggcc
     4081 gcttggattg acgcgggtac cgttcgcctc ctggggagtt aacatgagag tcttgttccc
     4141 gcgatggtac gtgcactcct tcgaaaattg ttcaccgagc ttcacctata cggtttgtac
     4201 agcctctacg tgaagggacc cttgcgtgac gacggctggt ttcgcagctt cgacgagggc
     4261 cgggccgtgg acaaagacgg caacccggta ccgttcatca catatcctgc catcgaattc
     4321 ctctcccggc ggatccatcc agaaatgtcg gttttcgaat tcggctcagg ttcgagcacc
     4381 ctgtggtggg cgtccagggt cagggaagtc gtcgcttgcg agcacgaccc aaaatggttc
     4441 ctggaaatcc gggcgcgggc gcgggaaaac gtcaccgtga tacaccatcc cctcgaatac
     4501 ggcggcgcct attcaaaagc catcctgaat ttcaaggacc gcttcgacat cgtcgtcgtt
     4561 gacgggcgcg atcgtgtgaa ctgtgcgaag aactgtttag gggcgctcaa attggacggg
     4621 gtcgtacttt gggacaactc cgaccggacg aagtacgagg agggctaccg ctttctgggc
     4681 gaccgtggat ttcggcggct cgatttcgtc ggcatgtgcc cgattatcaa cgaacggtcc
     4741 cagacttcga tcttctaccg ccgcgagaac tgcctcggga tctaatgagg aaccgcggcg
     4801 gaagcagcag cggtcctcct ccgtgttctc cgtggtgaat cactttcacg cgattccgta
     4861 caactccacc acccggccga cgttctccgt cagattgaaa cgctccgcgg cccgcttcct
     4921 ccccgcgcaa cccattcttt cgcggagatc cggatctcgc agaagggtga ggatgctcga
     4981 cgcggcctcc cccgcgtcgc cgcggttcac caggaagccc gtctcgccgt gatccaccaa
     5041 ttcagggatg ccgcccactc gtgtcgcaac aagaggtctc cccgtggcca ttgcttcggc
     5101 gatcacccag ccgaacacct cctcccagcg cgacaactgg cagacaacat cggcggctgc
     5161 gtagacgccc tcggcgaaag ggttttcgat gaggccggtc cacgtgatat ttccgcccag
     5221 acccgccgct tcggcttgac gctgatactc ttccctccag gaaccatcgc ccacaaccgc
     5281 gaagtggact tgcggctctt gttcgatcac gcgccgggct gcctcgatca ggtcggcgat
     5341 gcccttctct ggccggatcc agcttacctg gatgaccagt ggacgttcgg ccgggattcc
     5401 gtgtttcctg cggaattcat cccctgtgcc gccgaccatc gagaagtcca ccgcgttgta
     5461 aacccgctcg atcgcccgcg gtggtatcag atcatgcgat gtgagacagc gcctcccgta
     5521 ttcggagacg gacaacgttc gattcagagg agcattgatc agacgcacgg cggcccgctt
     5581 ccagagcgga gcgcgccgcg gcacgtaacc ctccggccat gacgactggt ccgtgaagaa
     5641 cacgcgctta acgccacaca gccgcgccag ccagggatac ggactgacga atccggtgaa
     5701 gtggagatgc agaatctccg gccggcaccg gcgcaaaagt cggaccagat ccgccgacgg
     5761 ctgccaagcg agactcaccg aaccgggcag agtctcaaaa gaaacgttcg acagcgccag
     5821 aaagcgccgt acggcttccg gtggctcact cagaaagcac aggatgctct cccagccgtg
     5881 ccgtcccagt tggctagaga gttcccgggc gaacgcctca ttgcctccaa tccggaacgg
     5941 ctcgacccca aaaaccgaca gtacggcgcg tctcatcgat cggaacggtt cactggacga
     6001 ccggaagcga cagagtttgt ttctccgcgt cggagcagcg gcgatcgagg cagcaacccg
     6061 cttcccaaaa gcgcggacgg ccctcccaaa tgcgccgggc cggcatggtt ttcacgttcc
     6121 cgaccggctt ctgcccgtta cacacggtca cgtctccatt tgcctgaaac tggagcgtcg
     6181 tgagcgcggc gcacagtgcc cgccgctcgt gcgccgcgtg gaactgcgtg gagacccgta
     6241 gcgaatcggg gttcctgaag tacgggatca tcgcctccag ttgctcgtaa ctgttgtcga
     6301 tgtgcagtcc gccgcgtttg agttcgatga gctgccggac cacttcggtc gccttctccg
     6361 tgtcccgcgg ccagttctcg ctcgtctcga accagcgcgg gtcctccggt gtattgtagt
     6421 tctgctcgat cggctggaag aagatgtgca ttccgtcctg agtggcgaac cgggcgactt
     6481 cgcaaactcc gtccaggttg tgggacatga ccaccgtctt cagccggacg tggtacggca
     6541 gccgcttctc tttccggacg cgaagaagcg tctgaatcgt cgtggacgtc ttctcgaaga
     6601 acgcgtcgcg gccgcgaatc ttcgtatgtg tgtcgccaat cccgtccagc gaaacggtca
     6661 ctcgccacgg cctcgccagc gccatccgct cgatcttcga ctgatcgtcc caatagccgt
     6721 gcgtcaggat ctcgagaaac aagccgagtg aggacgcgtg ggccacgaga tccgttgtga
     6781 acggcttgag caatgcctcg ccgccgctga agaccacgtg gacaggcccg agccagcggc
     6841 gcagatcggt cagcaccgtc ttccactgat cggccgtggg agtatcctcc cgtccgcggt
     6901 tcttccagat atcgcagtgc acgcaccgcg cgttgcacaa ctccgtcagc aggaaaatga
     6961 tcgaaactgg ccggcagcgc acggagaact gcccgccggc caatgtccgt agccggtaat
     7021 tcaccgcatc gtggaggcgt ttccgtaacc agttgatgcg aaaggacatg cctataaatt
     7081 ccggagctct ttcagatgct gcatctggaa tcataccacg gcatgtctga actggactct
     7141 cagctttggc agatcagttg caccaccagc tggacatagg atcagatctc ctccggcggg
     7201 actaattaca gcccaggcac agtttggttg cttttttcgt aaccttaatg aattgaatga
     7261 attgcaggtg atcttagccc ctgcgaaaga aaggacctcc acggatgccg gtaagtgtca
     7321 cccagcccag tagcgaaacc ctacgcaccc tcccgggcga cgatgtgcgc caaattatgt
     7381 ggcgcttcac cgatcggttc gacctccaga tgattgtcca gtccagccgc ggtgtggctc
     7441 gcggtcctgt cgccaggctc gtcgctgagg gcgcccgtaa tactcatgac tggaccgacg
     7501 caaaagccag ccttctcaag gcgttcgatg aatccggaat cacctccgtc ttcctcgatc
     7561 ctgagcaggg tggcttcatc gaagggccga agaacctcgc cctggcgctg gtcgccttcg
     7621 agctcgcctg ggtggacgcc ggcgccgcca ccggcagcct ggcgggctgc ctcgcgctcg
     7681 ctcccattca cgagcgcggc accgccgccc agcgcgatta ctacctgcgc ctctgcgcac
     7741 ccgccgcccc cggcgaggaa cgccagccgt ggcgcggcgc gttctgcctc accgagccga
     7801 ttcccttcgt tggcgtcgaa accggcttgc tcggcggcaa ggtacggatc gccgaatgga
     7861 aggaaggcga agaaccagtc cttcaggtcg acaagcgcgg gcgcttcatc accaacatgg
     7921 gcttcgccaa tttcgtcacc gccgccgtgg actcggccga cccgcgcatc aagggaagct
     7981 gcatggtcat cctgcacgag accgaccccg gtgtctggga ccgcggtacg cccgcgcgga
     8041 agcttgtcca ccagctttcc tcgacgaacg atccgatctt cggccagcgc gtccctgccg
     8101 gccgaatcat cggcggctac acagtgaagg acggcgtcat cgtaccccgg tacagccacg
     8161 gcgagatcat cgaggccgtc ttccggcgca cccgcgtcac cgtggcgctg atgacgtcgg
     8221 ccaaactcct ttccgccgtc gagccggtca tacgctacca gcggggccgt ttccgcggcg
     8281 gcgacatcgc cgagcccggt tcgccccggt acgagctcgg cctccagatg aaggaagacg
     8341 tgctccaccg cctcgtggac gtttgggcca ccggcgaggc cggcacttcg cttggctttg
     8401 aggccgcccg cctcttcgac cagctcgatc cgctcgagcg gaagaaggaa gagatcttcg
     8461 ctgcggaagg cgtcggccgc ggctcagccg cgttcaaggc gatgcgtcgg taccagaagg
     8521 acgcactcga ctgcctccag cacggcgtga actgcgacca gcccatgatc cagtatgtgg
     8581 ttctcgattc gctcgccaac gtgctctgcc ccgcatgcaa actctggaac accggccacg
     8641 gagctacgat gatgcgcgag gctgtcagcc tcatgggcgg ctacggtatc accgaggatt
     8701 gccccggctt cctcggccac aagtggatgg acgctcagct cgaagccacc tacgaaggcc
     8761 ccgaggctgt ccagcgccgg cagttgagcg tcaccatgac caacgagctg ttcctcgcgc
     8821 agttccacca gtggatcgcc gagatgcgcg atatcgccag caagcgcccg ggcaccggcg
     8881 cctgcacgct cgccactgcc atgcagctct ggctctggac gctgacttat ctgcaaaagg
     8941 ccactgaccc tgacggctcg aagctgtatc acagctcgcg ccagggtgtg accttcccgc
     9001 tcgctgacgc gctgtgctgg ctgctcgcct gtcgctactt catcctcgac gttctggaac
     9061 tgtcggaaaa aggtccggcc aacccggtcg tcgccgaggg ccttcccgga ctcctgaagt
     9121 tctacaccga tctctgccac gtccagagcg cccgtgccgc cggcgaagtc gctcgcgtct
     9181 gctcggagtt ggtttacggt taccggccgc atccggcgtg ggagaccccg gactacgcgg
     9241 gttgcttcaa gtccgacgag ctgatcgcca tcgaggccat ggtacccggc atgtcgagcg
     9301 ttgcaaccga cgtcgtcgaa agcgacggct cccatcccgc caaggagggc ccctgcgcga
     9361 cgttcgacga cgtcgagcag ttcatccgga ttcgtaaccg cctcgacggt tgcctgacag
     9421 gatcgcacct cgcgaaggag cgggccgccg acgcgctgac caaggtgatg atcccggagg
     9481 cgttggacta ccccgcatga gcgaagtcac cgtcgaacgc cagaccatcg aagtcgacat
     9541 tgtttgtgtc ggcttcggcc cggctacggg cggctttctt accgagcttt cgcgccggat
     9601 catgaatccc gatggcacgc cgcgcctcga gagccgagtc atgcccggca tgccccttca
     9661 ggtcatctgc tacgagcgcg cggacgacat cgggttcggt gtctccggca ttgtgacaaa
     9721 ggcgcgcgga attcgcgcca gttttccgga tctcgaccac acagagatcc ccatggccgc
     9781 gcccgtccgg gaggagaaag tgctctttct tctcgaccct gtcggcgcca gccgtcgctc
     9841 tgccgcattg cgtgccgccg acgtcgccgt ccgggcgatc ggctgcgaac acaatgccat
     9901 cgagctgccc tacacgccgg atttcctcca caaggacggc gggctgatcc tctccatcgg
     9961 tcagttcaac caatgggtcg gccagcgact gatggacgtt ggagcggtcc agatctggcc
    10021 cggcaccccg gtggcgcagc cactgattga agaggaccgc gtcatcggcg ttcggctgct
    10081 cgatcagggt gtcagcaagg ccggcgagcc ggaggtgggc ttcatgcccg gcatggacat
    10141 ccgtgctgcg ctcactgtcg tcggcgacgg ccccgtcgga ccgatcggcc ggcagctcac
    10201 cgagcgtttc ggcatgcccg aagggcacca ccagcacgag tgggccgtcg gcatgaagat
    10261 ggtcatcgac ctccccgagg ataccgccct cgaaccgggc ttcgtgctcc acaccttcgg
    10321 ttatcccgaa ccggagatct tcggcttcct ctatgtccat ccggatcgtg tcgcttctgt
    10381 gggcatcttc gtcccgtcct ggttcgacag ccccgtccgc acgtcctacc gctatctcca
    10441 gcactacatc cagcacccgt atctgtggcg ttatctgaaa ggcggacgtc tgcgctcatg
    10501 gggcgccaag tccttgcagg aatccgggaa actcggcgag ccgcatctcg ccggaaacgg
    10561 ctacgcccgg atcggcgaag ggtccggctc caccaatgtc ctcacgggtt cgggagtgga
    10621 tgaagcctgg actactggcg tgttgcttgc cgagggcgtc gccgagctcc tcgaggcccg
    10681 gcagccgttc acgaaggaga atctcgatcg tgcctatgtc gcgcgtcgcc gggcgagttg
    10741 ggtcgagtcc gagggcaagg tcgccgaaca cgctcgtatc ggcttccaaa agggcctcgt
    10801 caccggtctg ttgggcatgg ctttggctgg cctcacgaaa ggcaaggttg tgttacctgg
    10861 cgaggccgtg cctccctacg accgcctgcc taccttcgag gaatactacg ccggccgcat
    10921 ccctccgcta gaaatcgccc gcatccggca ggagtgtctc gcgcgcgggt cgtcggttca
    10981 tgaagcgctc atggagcgtt gcggctggcc gcccattcca tacgacggcc agttacttct
    11041 ctcgcagcag gacgcgctgc tggtcggcgg caaggtccag gcccctcccg gctacgccga
    11101 ccacgttgtc ttcctttatc cgagcctctg cgaaacctgt ggcacacgca tctgcatcga
    11161 ggtctgctcc ggtgaggcca tcacagcttc cgccgaaggt cccccgcaat tcgaccgcga
    11221 gaagtgcgtc cactgcggcg cctgcctctg gaactgctcg caggctctcg aagaggaccc
    11281 cgagcgcacc aacgtcgaat tccgcgccgg cgccggcggc ctccactcga cggagaacta
    11341 gctagagacg taactgttag ggagacagac ttggcgttac tccgggcttg ccggtgttgc
    11401 taagagcatc cagcggtccg cgaattccca ttgcttttca aacttcgctt gatccacgcg
    11461 attgggccgc tttcgcgtcg ggtcgttcag ccagacgtga ttgccttcgg ttccggtcag
    11521 gacagcgaag tgaagcccct tggaacgccc aggcttcaaa ccgacgatga ccggcctacc
    11581 cttcgacaga tggcgctcga ggtcgaccca ttcgccgcgg agtgtaaccg cacgatagcc
    11641 gtgatcctcg aggtatcgct tcatatcagc cagcaggatg cctcgccggt ccggctgata
    11701 gagctgctgg tacacctgtt cgggcgatgg ggcagtggca gactgcttcg ccggccgacc
    11761 gctccagtag tgctccacca tcgctacact cgccgcccca cagccattct tcttctgcgc
    11821 gaagaacgga acgtcgagcc gctggtttgc ggccccggcc agcgtggcaa cacagatcgc
    11881 agccacgagc ggatagcgta acacgattgc ctgcttcact tggcgatcag cacgatcacc
    11941 gcggctgcga gcgcgatgac gatgtaagtc aggtgctcgt tggacagcga accgcccaca
    12001 acctccggac cgggatcttc tgcgcgtgct gacaactcgg atagttcctc cgcggtaagc
    12061 aactctggcg agcccgtgcc cgtattggca ggagcggaag tcactgtcgc cggttcaggc
    12121 gctgccggcg agtcagcatt gctcggcgat gtactggctg cttccgccgc ccttcccaca
    12181 ggggctacaa gggatagaca aaggacaacc accaggatct tcgagagtag ctgatacaac
    12241 ggaaagtgcc tcatgatttt ctccagtctc ttattcttcc aaaacacgtc tcacagcgcc
    12301 accggacccg gacgcagggc cgcccgcgtc ccgggaaagt tttaccgggt gctagccgga
    12361 agtttcgcaa tgaaacgcct cagccgcctt ctgtttccaa tagcttacgg gcgtccacga
    12421 atcaccttgc acgcagctta ccgcaggcgc gagttcccgt gtaagtccgc ctccgctgca
    12481 tcggagcccc caaaggctca gtgagaactc cgcatatctc gaaggccatc aatcatttgc
    12541 acaatgacct gactttacga cgcttttgac cgcattttga cgccgcgtgt atggaggtag
    12601 cgctccccat gccgcaagct cggtctcgca atggcacacg gaccagcgag atcgaacaac
    12661 gagagggccc ttcgacgcct catcgccagc accttcctca tcgtcaccgc accggctttc
    12721 tgtgaaacgc acctccggca gatgacagta cgcgtttacg actatagcgg ggtcccggcg
    12781 aacgtccagg cggcagccag agatggagtc gccgcgatct tccaccgtgc gggtgtggag
    12841 ctgatgtggg tggactgccc catcacggcg ccctcgaata ctcgtcccgc cgcctgcgat
    12901 atcggcccgg tccaaggcct gttgacgttg aagatcgtcc ccgagtccat ggccggcggc
    12961 tttcgcttgc ctcgggacaa gttcggcctc gctgtccgtg aatatggcgc ctatgtcttc
    13021 tttgaccgtc tgcgcaacca caccgctgcc aaatcgctgc tgctgccgcc cattctgggc
    13081 atcgtcaccg cgcacgagct gggccatctc cttctggaga agggtcattc ggccacgggg
    13141 ctcatggcgg aaggtctgct cagcactcag tttcgggacg cggaaaaagg cgtctggccc
    13201 gcgttcaccc tggaccagat cgcatggata cagcggcaac cgaagccggc gttggtcgcg
    13261 ggtgccggga gcctcccctc ggggatgaat tagcggtagc gcttcggcct accggaaccc
    13321 ttccaccagc atcagatccg tgtctgtatc ctgcctttgg gcatagagca ggaaacgctc
    13381 atccggcgat accgcgagtc ccagatcgat cagacgttgg ggatgaaaaa gggtcgcgat
    13441 gtgtccggtg gccagatcga gacgccggat cgacgaccct tgtgctgcat ggtcgtgagc
    13501 gacgaagtac aggccttgcg ccgcgggtac gaagctgaag cccagagcgg attcaagcac
    13561 ctgcacttcc tctcctcccc cggcaggcat ccgccaaatc gaggtcccca aaaagtcctt
    13621 gctatagtag agatctcgcc catccgcgga ctcgaacgcc agccgcccgc ctttctgggt
    13681 aacctggact gccttcccgc ccgctgccgg catcttccaa atctgccagt ccttggaccg
    13741 gtcggacgcg aagtagatcc agcgcccgtc tctcgaccag ctcgccacac cgtcgtcggc
    13801 cggatcgtcc gtcagtcgct ttggagctcc tccgtccgca cgcacggtat agatatcgaa
    13861 gctgccctgc ttcgtcgagt cgaacacgag ctgctgcccg tccggagccc atctgggggc
    13921 tcccgtcatc gtcgccgcca tcgttgtcac ctggactggg ttcgaaccat ccgcgtcgca
    13981 cacccacacc tcgttcgtac cagacctcac ggagatgaac gcgactctct tgccatccgg
    14041 ggagtactgc ggcgcaaggt ccgagcgagt cgaagccgca aagggggtgg gttcgcttcc
    14101 ctcgcccttc ctcccgttga tcggcagcct cagaatgtcc gtattccacg tgaagtggac
    14161 gtaagcgagt cgattgccct cgcgggagat caccggaaac gcgcccccct ccccttggat
    14221 cgcaacacgg cggggagcgc tttctcccga cgccggcatg cgccatagct catccggctc
    14281 gccctcttga aacgaagaaa aaatgacctc cttcccgtca ggggtccagt tcgccgtgga
    14341 gcggaacaag ttgagtgagg tgagccgccg cggctcgcct gccggaacca tctgatccga
    14401 caccgtcacg atgtacagat ctccagccac ccggcttcgg aagaacacaa gacgggatcc
    14461 gtccggcgag aattccgcga aactgtcggc gctcggggcc tgcggattcg tcagctttcg
    14521 tttctctcca gtcgtgatgg acaccgcgta gatcgaacag ggagccccta gaggattctg
    14581 ttccgtaacc gctagccact ccccgctcgc gtgccaggaa agcccctctg aacacgggtt
    14641 gatcattgcc actttgcggt ccggcccgcc gatcgccggc ataaggaaga tgccgacctt
    14701 gttttcgggc agcaccttca tgaacgcgat ggaacgcccg tccggcgacc atgccggcat
    14761 cccttcgggg cccgggccgg tcgtcagccg caccgcgttc tccgtgccga tcatcttcac
    14821 atagacgtcg aggttgtcgc gattgggccc cgtccaggcg aaagctactt ggttgccgtc
    14881 gggagagaaa ctcggcatgt actcggaccc cacatacgtg gtcaggggga ccggtcttag
    14941 ctgcgtgtcg ggcttcctcg ctgtcgtcag atagtgccgc acggcgagga acgcagccgc
    15001 cagcgccaac accagggtca ccccgacata gaacagcacc cacgaccgcc ggcgcagagc
    15061 cgctgcctgg gtcgttactg cctccgccgc tgcaacctcc ggttcgccat tgcactgcgt
    15121 ttctatcggg tatacaaacc ggtatccacg ccgtggaaac
//
LOCUS       DATIFQ010000027        13061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1539301, whole genome shotgun sequence.
ACCESSION   DATIFQ010000027 DATIFQ010000000
VERSION     DATIFQ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13061
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539301"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..718)
                     /locus_tag="VLE22_01060"
     CDS             complement(56..718)
                     /locus_tag="VLE22_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB13013.1"
                     /translation="MSATKGNKIRILLLDDHALFREGLARLLGMELDFELLVPCASVD
                     EALRILDTTQVDIVLLDYDLGPETGSRLIVHAAERGLGGKLKVLVVTAGLSDAEAAEL
                     LRLGVAGIFLKHSAPNLLSTAIRKVMEGEAWLDQRFLRVLLAGPPTASADDRPPRLSE
                     REREVLRAVLEGQANKEIAGRLRVSESSVKATLQQLFLKTGVRTRSQLVRMALEKFRD
                     YL"
     gene            complement(715..2124)
                     /locus_tag="VLE22_01065"
     CDS             complement(715..2124)
                     /locus_tag="VLE22_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB13014.1"
                     /translation="MKLPDILSARQRLDRTTVLAVAGFLIASIAFADFLLSPAVSLGF
                     LYLYPIMMAALFVSRTELLGLAILCTLLREILGPFQWDWGVGVRAFFGLVSYFGLGLF
                     VVELARRRRQTLEHLRVVEEQERLRRAAEEQLKVLIETSPAAIITVDTVGRIQVANQA
                     AHQVLGSEDQPLTGEPVGAFLPLLAGVPQADGGGRVLRTTLECRGRRRDGEVFLAHIW
                     LSTFLTSDGPRLAAIVFDTSEDLRDREELGLHRLMTSSRVLVRAVSHEIRNLCAGIAV
                     VHANLRRVPGVAASEDFQALGTLVDGLGRLVSAELRPPSENSTVSVDLREVLDELRIV
                     IEPSLRDEEIEVRWKVAEDLPPVYADRHGLLHVFMNLANNSQRAMRGSEQKELAIAAT
                     VESGRVVVRFIDTGPGVARPEQLFQAFQQAAEGAGLGLYLSRALVRSFAGELRHEPRA
                     YGCCFAVELAAARSAAELS"
     gene            2717..3958
                     /locus_tag="VLE22_01070"
     CDS             2717..3958
                     /locus_tag="VLE22_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSB13015.1"
                     /translation="MPARRPAEIAQDILDRCLHGDLWPREPFHSLIDVCLSYHGEEAR
                     AGSDALFRILVEGLADRFEPRLSEVYAEMFAEAVCRVYPEWRPETLVTRYENVRKTRR
                     FDAKNDQIRTVFVLSRVTLGADIAITSLALDAAKRCFPSARIVLAGGPKTRDLFVSDP
                     KVDYIPITYGRSATFRERLSVWPELRQRLSEPNSIVIDPDSRLTQLGLLPVCPDESYY
                     LYESRAFGWDGADSLTDLMRRWLAETFDVEDASPYIAPAPQAIDPETVTVSLGVGENQ
                     AKRIADPFEETLLAGLVGRGANVLIDKGFGDEEALRAERLAARFPGVRTFQGSFAAFA
                     SVISGSRLYVGYDSAGQHAAAASGTPLVSVFAGFPCPRMFERWRPTGRGPIEIVRVDK
                     PDPDEVLERTLAAVDRLFALR"
     gene            3966..5129
                     /locus_tag="VLE22_01075"
     CDS             3966..5129
                     /locus_tag="VLE22_01075"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB13016.1"
                     /translation="MRLLSVLVLFCSLLSAQTALTPSEISAGWILLFDGQTLFGWTPV
                     GDAKWHAAGGVLVPEGESGWLRANTPFADFVLSCEFRTAADGNSGVFIRSAKEGAPHE
                     TGYEVQIWQQHPKFTTGSLVNHIAAKKAELKADAWNTMVIRAEGERFVVQVNGQKVLD
                     GRDAKSRIGHIGLQYNKDKKIEFRNIKLRPLGLAPIFNGKDLSGWRVVQPPQPPKEPA
                     EWTVKDGAIHVEKGGGQLETEGMYDDFVMQMDIRTNPKDAEHHPNSGVFFRGDANGYW
                     TGYESQIRNEYKGGDRTQPVDFGTGAIYNRVAARKVVANDGEYFTKTIVARGRQMGVW
                     VNGYAVTSWEDDRPEGMNARQAARLTAGTISLQAHDPTTNLDFRNIRLVRLPR"
     gene            complement(5145..8507)
                     /locus_tag="VLE22_01080"
     CDS             complement(5145..8507)
                     /locus_tag="VLE22_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB13017.1"
                     /translation="MGRRLTLPLFSLVLLIPVPALSAQSTFGGIVGIVTDASGALVPA
                     AAIKAREIDTNTLRSATTDAGGLYEILNLKPGRYEITAIKTGFATARIAEARLEARHT
                     LRADLSLQLAPHSETLVVAERLVPLINTETGIIAHTKTFDQITQLPLNYRGVTTSPLA
                     VLFAVPGVHQDAGDRPSIAGGMPAQVEYSLDGISTANVSFFGPNPDMYPSTEMLSELK
                     VTSVDNSAEFGQMGDVTVITKAGANYLHGSALWYHQNAALDAKSYGSPEKQAKVGNTF
                     GGSLAGPVFLPKLYSGRDKTFFFVDYEGNRRPGSFPVQHSVPTADMRSGNLSGVPGPP
                     AVDPLSGAPFPGNQVPLTQINSVARALLDNYYPLPNYNFDGTTNSNYRRLFRNAFDTD
                     GYDTRIDHILTSRQQLCARWSWKKQASLGYLPFLPESSVRSYNKNLVVSHNSSFTPRL
                     LNEFRFGVSLSTFAELFPVQGSDAVAVLRLQGLDLSHVPDGRGFPYFNFGDGTGFTAI
                     GHGRDGISRSSTYQYTDNLSWRKGRHALKFGADLRRLGYGNTARGGWNDDFGGWVFNQ
                     GTFSGNALADLLLGLPTTSNLAIIGPDFDERATHYHFYGQDAWRVNERLTASFGLRWQ
                     LHPAMMEASGNITNFNPVTRSVIVPDHSLPAAPGFLVAINACPGYTSEIPCTPMITAS
                     QAGLPQGLRRTYYGNWTPRFGIAWRPFANNKTVVRGGFGVFTHTVLGQFAGRLTAIHT
                     SDERTYANYQGPGLPPLFTLPQTYAGDFALPDLGSEDFGEAISPSYRDPRAYQWNVTV
                     ERELPNKSSLRLSYVGTRSIGLNLLTDLNQQHGGTTPFSASRRPYPQFNRLYSFENLG
                     FAAYDGAEAEFVHRFSRTLFFQSSYVFSKLLGNAGSSRGGAGGGSLFPGDAPVPLLIT
                     DRFNTRLDRGPLGASRRHRLLVNGIYQLPVGKRRKWGARMSSVPNAFVGGWDLSTVTL
                     VESGPFQTPTIAARFDQSNTGMLSRGVPLRPDRIGDGNLSHPTLENWYDIAAFVPPPA
                     GSGRFGNSGVGILRGPGLVTVAVGLFKSFPLSEQLHMRLEATFTNVANHPNFAAPPVN
                     VSQPVSFGKITSTVGQQNSGSRVGQIAARLDW"
     gene            complement(8749..9636)
                     /locus_tag="VLE22_01085"
     CDS             complement(8749..9636)
                     /locus_tag="VLE22_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13018.1"
                     /translation="MKSRLLPTIGAVAFSLSLLWSAPGIPALTFWAFTPIDVPGADRT
                     YARDMNSGGQIVGHFEKEGPPYVYRGYLLSNGVFTPLEIGDYSDARGINSGGDIVGIF
                     YTSIDDYVPHGFLLSSGIVTQIDFPGADFSFPQAINATGDIVGVYVQGDTDHAFLLRD
                     GVFTTVDYPGALYTNARGINAKGDIVGLYGVVDPDGRQRSHGFVLGADGTFTTINGPD
                     ALNGNVNGNNSRGQVVGEYSLPSAPGVRFGFLLGKDEYTLIDYPGATVTRPWKITPEG
                     RYIVGFYDDLHGFIASRKP"
     gene            complement(9796..10533)
                     /locus_tag="VLE22_01090"
     CDS             complement(9796..10533)
                     /locus_tag="VLE22_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13019.1"
                     /translation="MKSVFAVLMTMITVGLPVTAQGKGGCKSIPVQVTISPVDSFGVG
                     GATPSALQPDSGGPYVDAVNGVSATINSCSGSYDLLLNLTRSARSLTVDLGNIVGSPT
                     SYTPGWASAGPVPLGFFNVRNLFYNYSQNSTYSFTTRFISNLDGVAKGYSLGLVNPTT
                     DAYTAPPANPEMNDPITTSNVIVWHYAAGSNGYTVETWVAYPDTTQMDSNTANSVYVG
                     GLITTVHNATVSAGQYSVPFQITVQRK"
     gene            complement(10648..12690)
                     /locus_tag="VLE22_01095"
     CDS             complement(10648..12690)
                     /locus_tag="VLE22_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB13020.1"
                     /translation="MSLSWKFCIFALTALLGFSEESVRHLYERARLLQERNRNLDEAI
                     RIYGRVVERARDDRSLAARAQFSQGMLYLRLGRDEQARQAFRKVIMDFSDQPSLVREA
                     RTRLGVASMTAMSVRQVWATPDSDNCGGPSRDGRYLSFTDWSTGDVAIRDLATGENRR
                     VTHKDKGFVESLSGQAPSSVFAPDSKHIAYWWVDEPPRSYIGVAGIDGSAPRVLCRFD
                     NAGLIRLTDWSPDGKHILACLASENSPARIVLVSAANGSIRTLMQSDAWPGRMSFSRD
                     GKFILHDGRGSPGSDVYLLSIETGRDIPVVRHPAYDFAIGWSPGGEQILFGSDRTGPL
                     SLWTLAVKNGEPRGEPRLIRQDAGKIEPLGVTRTGVFYYWLKTGLLDLYSAAIDPETA
                     AVLDQPKAIGQRLVGSNRFPGWSPDGKSLLYQSEPPGLEPVIVIRSLDTGRERELKAE
                     PPLQFKIPRWNAAGDAILISGILDGKKGIYRMDPKSGAVALAVQLPADKELFVPSWSA
                     DGKILFGRFNDFDGVQRMDVATGTIETLYNPNDPPGPYHRGDTGPSDPEISPDGRTLL
                     FQQRDRPRSDNLLIMPAQGGTPRVLASGKWPERWFPFGAYAWTPDSRRILFVQRDGEP
                     SEASVISVIAAAGGELRRTGIRMNGIRQLRLHPDGKHIVFLAGKPGGEVWTIDNLF"
     gene            complement(12755..>13061)
                     /locus_tag="VLE22_01100"
     CDS             complement(12755..>13061)
                     /locus_tag="VLE22_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB13021.1"
                     /translation="PALLRFGDLVIDSGAHRVTKAGAEVILTRREFEILEFLARHPGQ
                     VIPRDRFYNDLWGETINVTERVIDTHIASLRRKLENDPANPQLILSVRGMGYKLRTN"
BASE COUNT         2619 a         3975 c         3999 g         2468 t
ORIGIN      
        1 cgaacaccga cctaatgctc atggagaatt tccgatgatt aggtgctagc gccccctaca
       61 ggtaatcccg aaacttctct agcgccatcc gtaccaactg gctccgcgtc cgcacccccg
      121 tcttcaggaa taactgttgt agcgtcgcct tcaccgaact ctccgatacc cgcagccgcc
      181 ccgcgatctc cttgttcgcc tgcccctcca aaaccgcccg caacacctcc cgctcccgct
      241 cgctcagccg cggcggacga tcgtccgcac tcgccgtcgg cggtcccgcc agcagcaccc
      301 gcagaaaccg ctggtccagc caggcttccc cctccatcac cttccggatc gccgtcgaca
      361 gcaggttcgg tgcgctgtgc ttcagaaaga tccccgccac ccccaaccgc aagagctcgg
      421 ccgcctccgc gtcgctcagc cccgccgtca ccacgagtac cttcagcttc ccgccgagac
      481 cccgctccgc cgcatggacg atcagcctcg accccgtctc cggtccgaga tcgtaatcga
      541 gcagcacaat gtcgacctgc gtggtgtcca gtatccgtag cgcctcgtcc acgctggcgc
      601 acggcaccag cagctcgaag tccagctcca tccccaacaa cctcgccaga ccctcgcgga
      661 acagcgcgtg gtcgtccaac agcaggatgc ggatcttgtt gcccttggtc gcgctcatga
      721 cagttccgcc gcgcttcgcg ccgccgccag ctccaccgcg aagcagcaac cgtaagcccg
      781 cggctcgtgc cgcagctccc ccgcgaagga ccgcacgagc gcccgcgaca aatacaggcc
      841 caggcccgcc ccctccgccg cctgttggaa ggcctggaac aactgctccg gccgtgccac
      901 ccccggtcct gtgtcgatga accgcaccac cacccgcccg gactccaccg tcgccgcgat
      961 cgccagctcc ttctgctccg aaccccgcat cgcccgctgg ctgttgttgg ccaggttcat
     1021 gaacacgtgc agtagcccat gccggtctgc gtacaccggc ggcaggtcct cggcaacctt
     1081 ccaccgcacc tcaatctcct cgtcccgcag cgacggctcg atcacaattc ggagctcgtc
     1141 cagcacctcc cgcagatcga cgctcaccgt gctgttctcc gagggtggcc gcaattcggc
     1201 tgataccagc cgtcccagcc cgtccaccag cgttcccagc gcctggaaat cctcgctcgc
     1261 cgcgactccc ggaacccgcc tcaggttcgc gtggactacc gcgatccccg cgcataagtt
     1321 gcggatctcg tgcgacaccg cccgcaccag cacccgcgag cttgtcatca gccggtgaag
     1381 ccccaactcc tcccgatccc gcagatcctc cgaagtgtca aacacgatcg ccgccaggcg
     1441 cggcccatcc gatgtcagaa acgtcgacaa ccagatgtgc gccaggaaaa cctcgccgtc
     1501 gcgccgccgc ccccggcact cgagtgtggt tcgcagcacc cgcccgcccc cgtcggcttg
     1561 cgggaccccc gccagcagtg gcagaaacgc gcccaccggc tcaccggtca gcggctggtc
     1621 ctcggagccg agaacctggt gggcggcctg atttgcaact tggatacgac ccacagtgtc
     1681 aacggttatg atcgcggctg gactcgtctc gatcagcact ttgagttgct cttccgccgc
     1741 ccgccgtaac cgttcctgct cctcgaccac ccggaggtgc tccagagtct ggcgccgccg
     1801 ccgagccagt tccaccacga acaggcccag gccgaaatag gataccaggc cgaaaaacgc
     1861 ccgcaccccc actccccagt cccattgaaa cgggccaagt atctcgcgca acaacgtgca
     1921 taagatcgcc agccccagca actccgtacg gctgacgaac agcgctgcca tcatgatcgg
     1981 gtaaaggtac aggaaaccga gagacaccgc cggacttaga aggaaatcgg caaatgcgat
     2041 gctcgcgatc aggaagccag cgacggcgag aaccgtcgtt cggtcgagcc gttgccgtgc
     2101 ggagaggatg tcgggcagtt tcatgggttt gacgcccgga tgctcacatc aggccatatt
     2161 atagcgtgct acgcccacgc cataggcaca agggaatagt actgttgagg cttcagtacc
     2221 aaagtactac tttttctatc gaaagataag cttcaagaga atgacaaaac ggtgtttact
     2281 ttaaataggt tcggacgtgc aatacgaacg gaaaacccgg gtcgggaggc cggggggtcc
     2341 gagaaccgaa ggttttgcgt tcactcctcc tgccggtatc aacgccggta ccaggctcat
     2401 cggtcctgct gcccggggcc gatgagcctt tctttattcc ctgtattctc agtggagaga
     2461 aattcgcccg gtcggcgact atgcggtgtt aggccggagg cctacacaca acgtccttcg
     2521 ctgtcgatag aaccagtgag aggacacgac ggatggattc gatgccactg taccaagccc
     2581 ttctggctgt catgctaggt ttcgccctga tcttgcgcgg agagaaaatc cgcagacggc
     2641 gtgaccgccg cgagcgcctg ctgcgtgcgc tccgatcact ttccgaggcc acgcccggcg
     2701 cattacaatc gtagggttgc ccgctcgacg acccgccgag atcgcccagg acatcctgga
     2761 tcgttgtctt cacggcgacc tgtggccccg tgagccattt cattcgctga tcgacgtctg
     2821 ccttagctac cacggcgaag aagcccgcgc gggaagcgac gccctgttcc ggattctggt
     2881 cgagggtctg gctgatcgat tcgaacctcg cctgtcggaa gtctatgccg agatgtttgc
     2941 cgaggccgtc tgccgcgtat accccgaatg gagaccggaa accctcgtca cacgctacga
     3001 aaacgtgcgc aaaacgcgcc gttttgatgc aaaaaatgac caaattcgca ccgttttcgt
     3061 cctctccaga gtcaccttag gcgccgacat tgccatcaca agcctcgcgc tcgacgccgc
     3121 gaagcgatgc ttcccctcgg cgcgaatcgt cctcgccggc ggccccaaga cccgcgacct
     3181 cttcgtatcc gatccaaaag tagattatat tccaatcact tacggacggt ccgcgacgtt
     3241 ccgcgaacgc ctttcggtct ggcccgagct acgccagcgc ctgtcggagc caaacagcat
     3301 cgtaatcgac ccggattcgc ggctgaccca gcttggtttg ctgccagtct gccccgatga
     3361 atcctactat ctatatgaaa gtagggcatt tggctgggat ggcgccgact cgctaaccga
     3421 cttgatgcgg cgctggctcg ccgaaacatt cgatgtcgaa gacgcctccc cgtatatcgc
     3481 accggcgccg caagccattg atcctgagac ggttacagtc agcctcggcg tcggagagaa
     3541 ccaggccaag cgcatcgccg atccgttcga ggagacgctg ctggcaggct tggtcggtcg
     3601 cggagctaat gtattgatcg acaagggttt cggcgacgag gaggcgctcc gtgccgagcg
     3661 tttggcagcg cgttttccgg gcgtccggac cttccagggg tcgttcgcgg ccttcgcctc
     3721 cgtgatctcc ggaagccgcc tctacgtcgg gtacgactcg gccggacagc acgccgcagc
     3781 ggcctcgggg acgccgctgg tcagcgtttt cgcgggtttt ccgtgtccgc gaatgttcga
     3841 gcggtggcgg ccgacgggcc gggggccgat cgaaatcgtg cgcgtggata aaccggatcc
     3901 ggatgaggtg ctggaacgga cgctggcggc cgttgaccgg ctttttgcgt tacgatagag
     3961 ccgctatgcg actcctatcc gtgcttgtgc ttttctgctc gctcctttcc gcgcaaacgg
     4021 cgctgacacc atcagaaatt tccgcggggt ggattctgct gttcgacggc cagacacttt
     4081 tcggctggac gccggtgggc gatgcgaaat ggcacgctgc gggcggcgtc cttgttccgg
     4141 agggcgaatc tgggtggctc cgggcgaaca ctccgttcgc cgatttcgtg ctgtcgtgcg
     4201 agttccggac ggcggcggac ggaaacagcg gggtcttcat ccgttcggcg aaggaaggcg
     4261 cgccgcacga gacggggtac gaggttcaga tctggcagca gcaccctaag ttcacgaccg
     4321 gcagcctggt gaatcacatc gcggcgaaga aagcggagct gaaagcggac gcatggaaca
     4381 cgatggtgat tcgcgcggag ggcgagcggt tcgtcgtgca ggtgaacggg cagaaggttc
     4441 ttgacggccg cgatgcaaag agcaggatcg gccacatcgg acttcagtac aacaaggaca
     4501 agaagatcga gttccgaaac atcaaactgc ggccgctggg gctggcaccg attttcaacg
     4561 ggaaggacct gagcgggtgg cgggtggtgc agccgccgca gccgccgaag gagccggcgg
     4621 agtggacggt gaaggacggg gcgatccacg tggagaaggg cgggggacag ttggagacag
     4681 aggggatgta cgacgatttc gtgatgcaga tggacatccg gacgaatccg aaggacgcgg
     4741 agcatcaccc gaacagcggg gtgtttttcc gcggggatgc gaacgggtac tggacggggt
     4801 acgagtcgca gatccggaac gagtacaagg ggggcgatcg gacgcagccg gtggattttg
     4861 ggacgggggc gatctacaac cgggtggcgg cgcggaaggt ggtagcgaac gacggggagt
     4921 atttcacgaa gacaatcgtg gcgcgtgggc ggcagatggg ggtgtgggtg aacgggtacg
     4981 cggtgacgtc gtgggaggat gaccggccgg aggggatgaa cgcgcggcag gcggcgcggc
     5041 tgacagcggg gacgatcagc ctgcaagcgc acgacccgac gacgaacctg gacttccgca
     5101 acatccggct ggtgaggctg ccgaggtaag ttgcgctgcg gcgtctacca atccaaccga
     5161 gcggcaatct ggcccacgcg actgccgctg ttctgctggc ccaccgtgga tgtgatcttg
     5221 ccgaagctca ctggctggga aacgttcacc ggcggtgcgg cgaagttagg atgattggcg
     5281 acgttagtga acgtggcctc cagccgcatg tgaagctgtt cggaaagcgg gaaactcttg
     5341 aagagaccca cggcaacggt cacgagcccc ggtccccgga ggatgccgac cccggagttc
     5401 ccaaagcggc ccgatccagc cgggggcggg acaaacgccg caatgtcata ccaattctca
     5461 agcgtggggt gagagagatt tccgtctccg atccggtccg gtcgaagcgg gacacccctg
     5521 gagagcatac cggtgttgga ctggtcgaag cgggcggcga tcgtcggcgt ctggaagggt
     5581 ccgctctcga cgagcgtgac ggtgctgaga tcccaacctc ccacgaaggc gttcggcacc
     5641 gagctcatgc gagcacccca cttgcgccgt ttgccgacgg ggagttgata gatgccattt
     5701 accaggagcc tgtggcggcg ggacgcgccc agtgggcctc ggtcaagccg tgtattgaac
     5761 cggtctgtaa tcagcaacgg gacgggcgcg tcacccggaa aaagcgagcc gccaccggca
     5821 ccgccacgcg atgagccggc attgccgagc agttttgaga agacgtaact ggattgaaaa
     5881 aaaagggtcc tactgaatct gtgaacgaat tccgcttcgg cgccgtcgta agccgcgaag
     5941 ccgagatttt cgaaggagta tagccggttg aactggggat aaggccgccg gctggccgag
     6001 aatggcgtcg tgccgccatg ctgctggttc agatccgtga gcagatttaa accgatcgaa
     6061 cgcgtcccga cataactcaa ccgcagcgag cttttgttcg gcagctcccg ctccacggtg
     6121 acgttccact gataagccct gggatcccgg tagcttggag agatggcttc accaaaatct
     6181 tcgcttccga ggtccggcaa tgcaaagtct ccggcgtagg tttgaggcag agtgaacagg
     6241 ggcggcagtc ccgggccctg gtaattggca taggttcgct cgtccgatgt gtgaatcgca
     6301 gtcagcctcc cagcgaactg tcccaagacg gtgtgcgtga agacgccgaa gccgccgcgg
     6361 accactgtct tgttgttcgc gaacggacgc caggcgattc cgaatcgtgg cgtccagttt
     6421 ccgtagtagg ttcgacgaag gccttgcggc agtccggcct ggctggcagt aatcatcggg
     6481 gtgcacggaa tctcactcgt atagcctggg caggcgttaa tcgcaaccag aaatccgggc
     6541 gccgccggca gcgaatggtc gggaacgatg acggagcggg tcaccggatt gaaattggtg
     6601 atgttgccgg acgcctccat catggcggga tgaagctgcc agcggaggcc gaaacttgcc
     6661 gtcaggcgct cgtttacgcg ccacgcgtcc tggccgtaga agtgatagtg ggtcgcccgt
     6721 tcgtcgaagt ctggaccaat gatggcgagg ttggaagtcg tgggcaatcc gagcagaaga
     6781 tcggcaagtg catttccgct gaacgtgccc tggttgaaga cccacccccc gaagtcatcg
     6841 ttccatcccc cgcgggccgt gttcccgtaa cccagccggc ggagatcggc accgaacttc
     6901 agggcgtgcc gtcccttccg ccagctcaga ttgtcggtgt actggtaggt gctggagcga
     6961 gagatgccgt cgcggccgtg gccgatcgcc gtgaagccgg taccgtcgcc gaagttgaaa
     7021 taggggaaac cgcggccatc gggcacatgg ctcaggtcca ggccctggag cctcagcacg
     7081 gcgactgcgt cgctgccttg aaccggaaac agttccgcga aagtggacaa gctgacgccg
     7141 aatcgaaatt cgttcagcag gcggggcgtg aacgagctat tgtgggaaac aaccagattc
     7201 ttgttgtaag agcgcacgct gctttcgggc agaaatggga gatatcccag gcttgcctgc
     7261 tttttccagc tccaacgggc gcacaattgc tgccgcgaag tgaggatatg atcgatccgg
     7321 gtatcgtatc cgtcggtatc gaacgcattg cggaacagcc gccggtaatt gctgttggtg
     7381 gtcccgtcaa agttgtaatt cggcagcggg taataattat cgagcaaggc acgggccacg
     7441 gaattgatct gagtgagggg aacttggttt cccggaaacg gcgccccgga aagcgggtcc
     7501 actgccggag ggccaggcac accgctcagg ttgcccgagc gcatgtccgc cgtaggcacc
     7561 gagtgctgca ccggaaagga gccgggccgg cggttgcctt cgtaatcgac aaaaaagaag
     7621 gtcttgtcgc gccccgagta gagctttggt aagaagaccg gtccggcgag gctgccgccg
     7681 aaggtgttac ctactttggc ttgtttctcc ggcgacccgt aggatttggc atccagcgcc
     7741 gcgttctggt gataccacag tgcgctgccg tgcaaataat tcgcgccggc cttggttatc
     7801 accgtgacgt cccccatctg gccgaactca gcgctgttat cgaccgaggt cactttgagc
     7861 tcgctcaaca tctccgtcga ggggtacatg tcggggtttg gaccaaaaaa cgagacattg
     7921 gcagtggaaa ttccgtctaa gctgtactcg acttgggccg gcatcccacc ggcgatcgag
     7981 ggcctgtcac cggcatcctg gtgcacaccg gggacggcga agaggacggc aaggggacta
     8041 gtggttacgc cgcggtaatt cagcggcagt tgcgtgatct ggtcgaaggt cttcgtgtga
     8101 gcgatgatcc cggtttctgt gttgattaga ggaaccaggc gctcagcaac cactagcgtc
     8161 tccgagtgcg gcgccaattg caggctcagg tcggcgcgca gagtatgccg tgcctccagg
     8221 cgggcctccg cgatcctcgc ggtggcgaat ccagttttga tggcagtgat ttcgtagcgg
     8281 cccggcttca ggttgaggat ctcgtacagt ccgccggcgt ccgttgtggc ggagcggagt
     8341 gtgttcgtgt cgatctcccg ggccttgatg gcagccgctg gaaccagcgc gcccgaggcg
     8401 tcagtcacta tgccgacgat accgccgaac gtggactggg cggacagggc gggcacggga
     8461 atcaacaaca ccagcgagaa taggggcagc gttaacctgc gtcccaagaa ctcctcccag
     8521 atactgagat tctaacgcag gtggctgttg ttgtccgcaa ggctttcgtg aagtcttgat
     8581 catgttgacg gctagtcgca gccgccagac ccgaccgatc catcccaggc atcgaactgg
     8641 aacttacaca agctgactcc atcactcaca cgtcgcctcc ggggctgtcc agccaggacc
     8701 acgatgtgtt ttccgctgga ttggtttgtc aggtacggtg cgttcctttc atggcttgcg
     8761 actcgcgatg aagccgtgta gatcgtcata gaacccgacg atgtaccgcc cctcgggtgt
     8821 gatcttccaa gggcgcgtta cggtcgcacc cggatagtcg atcaaggtgt actcatcttt
     8881 cccaagaaga aacccaaagc gaactccggg cgcgctgggc agcgagtact cgcctacaac
     8941 ctggcctcga gaattgttcc cgttgacatt gccattcagg gcgtcagggc cgttgattgt
     9001 ggtgaatgtg ccgtcagcgc ccagcacaaa accgtgggac cgttgccgac catccgggtc
     9061 gacaacccca tagaggccta cgatgtcacc cttggcgttg atgccacgag cgtttgtata
     9121 aagcgcaccg ggatagtcga ccgtggtgaa cacaccatcg cgcagcaaga acgcatggtc
     9181 cgtatctccc tgtacataga ctccgacgat gtcacctgtg gcgttgatcg cctgcggaaa
     9241 actgaagtcc gcgcctggga agtcgatctg agtgacgatg ccgctgctca acaagaaacc
     9301 gtgcggcacg tagtcgtcaa tggaggtgta gaagatgcca actatgtcac caccgctatt
     9361 gattccgcgc gcgtcactat agtcgccaat ctcaagcggc gtgaagacac cattgctgag
     9421 aagataccca cggtagacgt acggcggtcc ctccttctcg aagtggccta cgatctgacc
     9481 tccggagttc atgtcgcggg cataggtccg atcagcgccc ggcacatcga tcggagtgaa
     9541 cgcccaaaat gtgagggccg ggatgccggg ggcactccat agcaatgaaa gtgagaacgc
     9601 aacggctcct attgtgggca aaagtctgct tttcatgtgt gatctcctaa taatcattaa
     9661 ccatgtccac gatggggccg ctaccgccgg ccaacatcag cgcggagcag ggctccggat
     9721 cacgccaggc ggcaccgcga ccgttgtaat gcgcctccag ttcgcgaaca acatgcgggg
     9781 cgctgaaata gggacttact tgcgctgcac agtgatctgg aacgggacgc tatactggcc
     9841 ggcgctcacg gtcgcattgt gaacggttgt gatgaggccg cctacatata cagaatttgc
     9901 agtgttgcta tccatttgcg tagtgtccgg ataggcaacc caagtctcca ctgtgtatcc
     9961 gttagatcct gccgcatagt gccacacgat gacgttggag gtcgtaatcg gatcgttcat
    10021 ctccggattt gccggcggcg cggtataggc gtcagtggta ggattcacca gaccgaggct
    10081 gtaccctttc gcgacgccat cgagattcga gatgaaccgc gtagtgaacg agtatgtcga
    10141 gttctggctg tagttgtaga agaggttgcg aacattgaag aaacccaatg ggactggtcc
    10201 agccgaagcc caaccgggag tgtaagatgt gggtgaacca actatgtttc ccaggtctac
    10261 ggtgagcgaa cgggcgctcc ttgtgagatt caggagcagg tcgtaggacc cgctacagct
    10321 attgatcgtc gctgaaactc cgttgaccgc gtccacgtaa ggccctccgc tatccggctg
    10381 cagcgcagat ggagttgccc cgccgacgcc aaagctgtcg acaggactaa tcgtcacttg
    10441 aacgggaatg ctcttacagc ctccttttcc ctgggccgtt accggcaggc ccacggtgat
    10501 catcgtcatc agcactgcaa aaacgctttt catctttctc tcccaggaag taattcacat
    10561 cccaacgcaa caatcttact ggcaggggat atgaagtgtg tcaggattga atgagaatta
    10621 cgccaaagtt tggcgaaggg atgaacctca gaaaaggttg tcgatggtcc acacttctcc
    10681 cccgggcttt ccggccagga atacgatgtg ttttccgtcg ggatggaggc gcagttggcg
    10741 gatgccgttc atgcgaatgc cggttcttcg caactcacct cccgcggctg cgatcacgct
    10801 gattacgctg gcctcggaag gctcgccatc gcgctgcaca aagaggatgc gacgcgagtc
    10861 cggagtccag gcgtacgctc cgaatggaaa ccagcgctcc ggccacttgc cgcttgccaa
    10921 tacccggggt gtgccgccct gtgcgggcat gatcagcagg ttgtcgctcc gcgggcggtc
    10981 ccgctgctga aacaggagcg tgcgtccgtc gggcgagatc tcggggtcgc taggccccgt
    11041 gtcgccgcgg tgataaggac caggcgggtc gttggggtta tacagggtct ctatcgttcc
    11101 cgtggcaacg tccatgcgtt gaacgccatc aaaatcgttg aaccgcccga acaggatctt
    11161 cccgtccgcc gaccaggaag ggacgaagag ctctttgtcc gccggcagtt ggacggccag
    11221 cgcaaccgcg ccgctcttcg ggtccatccg gtagatgccc ttctttccat ccaagatccc
    11281 cgagatcaaa atcgcatcgc cggccgcatt ccaacgtgga atcttgaatt ggaggggagg
    11341 ctcggccttg agttctcgtt ctctgccggt gtccagggag cggatcacga tgacgggttc
    11401 gaggccaggg ggctcggact ggtagaggag cgacttgccg tcgggtgacc agccgggaaa
    11461 ccggttcgat cccacgagcc gctgcccgat cgctttcggc tgatcgagga cggccgcggt
    11521 ttcaggatcg attgccgctg agtacagatc gagcagccct gtcttcagcc agtagtagaa
    11581 tactccggtt ctggtcacgc ccaagggttc gattttgccg gcatcctgac gaatcaaacg
    11641 tggttcaccg cgtggctcgc cgttcttcac tgccagcgtc cacaggctga gcggaccggt
    11701 ccggtcgctt ccgaatagaa tctgctcgcc gccgggcgac catccgatgg caaagtcata
    11761 cgcgggatga cgcaccacgg gaatgtcacg gccggtttcg atggaaagca gatagacgtc
    11821 cgatccgggg gatccacgac cgtcatggag gatgaacttg ccgtcgcgcg agaagctcat
    11881 ccgtccaggc caggcatcgg actgcatcag cgtgcgaatg gaaccgttcg ctgccgagac
    11941 cagcacgatc cgtgcagggg agttttcgga tgcgagacag gcgaggatgt gttttccgtc
    12001 cggagaccaa tccgtgagcc ggatcaggcc cgcgttgtca aaccggcaca gaactcgggg
    12061 cgccgagccg tcgatgcccg cgacgcctat gtaagaccgt ggcggctcat caacccacca
    12121 ataggcgata tgcttcgaat ccggagcaaa caccgaggat ggcgcctgcc cgctcaaaga
    12181 ctccacgaag cccttgtctt tgtgcgtgac acggcggttc tcgccggtgg caagatcgcg
    12241 gatggcgaca tcacccgtgg accagtcggt gaaggagagg taacggccgt cccgcgaagg
    12301 ccctccgcaa ttatcactgt ccggagtagc ccaaacttgg cgaacgctca tggcggtcat
    12361 cgaggcgacc ccaagccggg tgcgagcttc acgcaccaga gaaggctggt ccgaaaagtc
    12421 catgatcacc ttacgaaacg cctgcctggc ctgttcgtcg cggccgaggc gcaagtagag
    12481 catgccctgc gagaattgcg cgcgggcggc cagagagcgg tcgtccctgg cgcgttcgac
    12541 cacacggcca taaatccgga tcgcctcgtc gagatttcga ttcctctcct ggagcaggcg
    12601 cgcccgctca tagaggtgcc ggacgctctc ctcggagaaa ccaagcagag ccgttagtgc
    12661 gaagatacag aacttccaag acagtgacac tgatgcggag tgtattctag ctctgctgca
    12721 cttgtggttc gtcaagattc tgtcaggctt ccaatcagtt tgtgcgaagt ttgtaaccca
    12781 tgccgcgcac gctcaggata agctgagggt tggcggggtc gttctcaagt ttccggcgca
    12841 acgacgcgat gtgcgtatcg atcactcgct ccgtcacgtt aatggtctcg ccccagagat
    12901 cgttatagaa cctgtcccgc ggaatgactt ggccgggatg ccgagccagg aattccagga
    12961 tttcaaactc ccttcgggtt aagatgacct ctgcgccggc tttggtgacc cgatgggcgc
    13021 cggaatcgat gacaagatcg ccgaagcgca acaatgccgg c
//
LOCUS       DATIFQ010000028         2931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1348887, whole genome shotgun sequence.
ACCESSION   DATIFQ010000028 DATIFQ010000000
VERSION     DATIFQ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348887"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VLE22_01105"
     CDS             complement(<1..522)
                     /locus_tag="VLE22_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13022.1"
                     /translation="MLTVLLILCGAAIPVFPAAPDKVLPAINEIDPKHREGQQPYEMT
                     WVQRSEDPRTLIDFEDMQGWTLELHDGAAGELRRSKEEQMWGQYTAKVVYSGGRPGSR
                     VVARPPKPVPIPGKFNAIDMWGYGDRRRYSSAQSGTGVMIAILVTDARGKEFRFEITD
                     VRWVGWWLIHRRAA"
     gene            complement(537..>2931)
                     /locus_tag="VLE22_01110"
     CDS             complement(537..>2931)
                     /locus_tag="VLE22_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13023.1"
                     /translation="RYFYCDSLAVYSEDLKALTLKPQPKRNLTPFRGQIAGLNTGTGK
                     LPFPTREETILPENFERDFLVSVRETAAGRFEFRYEGKDATLVYEYRPRTGNLAELTA
                     SLNGGTPYRPMENGGVRFAGTPEGHVAEGRLLSAALDGDVVRAKFAIGSNVVDYEMRL
                     WQKSLVLDAWCDGGNAAELSFGRVAGITNPKLIRNPYITYRAMSFLYPRVLVSGTESA
                     PVFTSVWFDWYRSNASEPFYKDTPEVTATTAEINGGMRYNPKTDGQRNNLCERVFLTV
                     SPRYEETLPTIANPASIRQRDGKQVLWTVSSPETFQKDHALSKRIRSYGIDKVMQHSH
                     EVTWRDDGDSFTLRDRAAPQKGGDAALQWYIRAQGDLGWLQGTYSNYTDFATVNSNWN
                     PDWVQRMPNGEWRRAWYRCYALKPSKAVEFDEYYARRIKDRYDIKMSYTDVHSSIAPW
                     VYCDYDARVPGAGTFAATYYAYGQILLNDQQVYGPTQSEATLQWMYAGLASGHYGWIQ
                     TRVNLLTEPLDVAFKLMKIHPLECDYGMGYTYFYLEQMDKEWMKSPKRRELVDLFLAT
                     TIGYGNMGWLVKEWGLDEPFGVEVLARSYYMLQQLQQQYAFVRPKTIEYADRTGRMLT
                     PSQAHATGAISDSRLHVEYENGTHVYVNRATDGAWKVKDHTGREVELPVTGWLAFNPN
                     NGFYETSANISGRRVDHVKAPEFEFLDGRGRWTERGSLGATGSVARCDRASGVIELID
                     IYGNDRIAFQSPSAGRLTAYDEEGKSLGMVDLTNPRAGWSEFKPVSRGRTYAFVAGQ"
BASE COUNT          506 a          863 c          983 g          579 t
ORIGIN      
        1 ggcggcacgg cggtggatca gccaccaccc cacccagcgc acgtcggtga tttcgaaccg
       61 gaactctttg ccgcgcgcgt cggtaaccag gatcgcgatc atgacgcctg ttccgctctg
      121 ggcggagctg tagcggcgcc ggtcgccgta gccccacatg tcgatggcgt tgaacttgcc
      181 ggggatgggg actggcttcg gcgggcgcgc cacgacacgg ctgcccggcc tgccgccgga
      241 gtatacgacc ttcgcggtgt attgacccca catttgctct tctttggagc ggcgcagttc
      301 gccggcggcg ccgtcgtgga gttccagagt ccagccctgc atgtcttcga agtcgatcag
      361 cgtgcgcggg tcctcggacc gttggaccca ggtcatctca tagggttgct ggccctcgcg
      421 gtgtttcgga tcgatctcgt tgatcgcggg aaggactttg tccggggcgg cgggaaacac
      481 gggtatggcc gcgccacaaa gaatgaggag aacggtgagc attcgcatgg aactccttat
      541 tgcccggcga cgaaggcata cgttcgcccg cgtgaaaccg gcttgaattc ggaccagccg
      601 gcgcgcgggt tcgtgaggtc aaccataccg agagacttgc cttcctcgtc gtaggccgtc
      661 aagcgacccg cggagggaga ctgaaaggcg atacggtcat tgccgtaaat gtcgatcagt
      721 tcgatcacgc cggaggcgcg gtcgcagcgg gcgacgctgc cggtggcgcc aagagaaccg
      781 cgttcggtcc accgcccgcg gccgtcgagg aattcgaact caggcgcctt tacatgatcg
      841 acacggcggc cggaaatgtt tgccgacgtt tcatagaaac cgttgttcgg gttgaaagca
      901 agccagcccg tgacgggaag ctcgacctca cggccggtgt gatccttgac cttccacgcg
      961 ccgtctgtcg cgcgattgac gtaaacgtgc gttccgtttt cgtactcgac gtgaagcctg
     1021 gaatcgctaa tagcgcccgt cgcgtgcgcc tgactgggcg tgagcatccg gcctgtgcgg
     1081 tccgcatact cgatggtctt cgggcggacg aaggcatact gctgctgaag ctgctggagc
     1141 atgtagtagg accgcgctag gacctcgacg ccgaacggct cgtcgagccc ccactctttt
     1201 accagccagc ccatgttgcc gtagccgatc gtggtggcga ggaagaggtc caccaactcg
     1261 cgacgcttcg gcgacttcat ccactccttg tccatctgct cgaggtagaa gtaggtgtag
     1321 cccatcccgt agtcgcattc gagagggtgg atcttcatta gtttgaaggc gacatcgagg
     1381 ggttcggtga gcaggttgac gcgggtctgg atccagccgt agtgcccgga ggcaaggccg
     1441 gcgtacatcc actggagggt cgcctcggat tgagttggcc cgtacacctg ctggtcgttc
     1501 agcagaatct ggccgtaggc gtaatatgtg gcggcgaagg tgccggcgcc gggcacgcgc
     1561 gcgtcgtaat cacagtaaac ccacggcgcg atggaggaat gcacgtcggt gtaagacatc
     1621 ttgatgtcgt agcggtcctt gatacgccga gcgtagtatt cgtcgaactc gacggccttg
     1681 gacggcttga gcgcgtaaca gcggtaccat gcgcgccgcc actcgccatt gggcatgcgc
     1741 tgaacccagt ccgggttcca gttggagttg acggtagcga agtcggtgta gttgctgtac
     1801 gtgccctgga gccagccgag gtcgccctgc gcgcggatat accactggag cgccgcgtct
     1861 ccgcctttct gcggcgccgc gcggtcacgg agagtaaaac tgtcgccgtc gtcgcgccag
     1921 gtgacttcgt ggctgtgctg catcaccttg tcgataccgt aggaccggat gcgcttgctg
     1981 agagcgtgat ccttctggaa ggtctccggg gaagagacgg tccacagcac ttgcttgccg
     2041 tcgcgctggc ggatcgatgc cgggttggcg atcgtcggca gggtctcctc gtagcgcggg
     2101 gagacggtga ggaagacgcg ctcacagagg ttgtttcgtt gtccgtcggt cttcgggttg
     2161 tatcgcatgc cgccgttgat ctcagcggtg gtggcagtca cctcaggcgt gtctttatag
     2221 aacggctcgg aggcattgga gcggtaccag tcgaaccaga ctgaggtgaa caccggcgcc
     2281 gactcggtac cggagacgag cacgcgcggg tagaggaagc tcatggcgcg gtacgtgatg
     2341 tacggattgc gaatgagctt cgggttggtg atcccggcga cccggccgaa actgagctcg
     2401 gccgcattcc cgccgtcgca ccacgcgtcg aggacgagcg atttctgcca caaccgcatc
     2461 tcgtagtcca cgacgttcga gccgatggcg aatttcgccc tgacgacgtc gccgtccagg
     2521 gccgccgaca acaggcgacc ttcggcgaca tgcccctccg gagtgcccgc gaaccgtacc
     2581 ccgccattct ccatcggccg gtagggcgtc ccgccgttga ggcttgcggt cagttctgcg
     2641 aggttaccgg tgcgcgggcg gtactcgtaa acgagcgtgg catcctttcc ttcataacgg
     2701 aactcgaaac ggccggccgc ggtctcgcgg acgctgacga ggaagtcccg ctcgaagttc
     2761 tcgggcagga tggtctcctc gcgagtcgga aagggaagtt tgccggttcc ggtgttcagt
     2821 ccggcgatct ggccgcggaa gggtgtcagg tttcgtttcg gctgcggctt gagtgtgagg
     2881 gccttgagat cctcggagta gacggcgagc gaatcgcagt agaagtaccg c
//
LOCUS       DATIFQ010000029        17418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2306653, whole genome shotgun sequence.
ACCESSION   DATIFQ010000029 DATIFQ010000000
VERSION     DATIFQ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17418
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2306653"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..731
                     /locus_tag="VLE22_01115"
     CDS             312..731
                     /locus_tag="VLE22_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13024.1"
                     /translation="MDWSIRVETDLFEHREAKPHFINPCCFGEDFAGWLKQQLSPLAG
                     SGYSFSESIQEDYGWGFGASRGKDRFWVAISYMGDGPQEPPAQWVVSVNHDPGLNPIK
                     RLLYKPDPAALAELRDRVQRALGANGAIRTIGPVETH"
     gene            900..1334
                     /gene="rplM"
                     /locus_tag="VLE22_01120"
     CDS             900..1334
                     /gene="rplM"
                     /locus_tag="VLE22_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HSB13025.1"
                     /translation="MNTVFPSGNEIKRNWFVLDAGDQVLGRVASKAAMILMGKHKPGY
                     APFLDTGDHVVVINASKIRLTGRKEQQKVYRSHSGYPGGLREVSVAVMRAKRPAKIVE
                     EAVAGMLPKTKLGKHMLKKLKVYAGERHPHQAQKPVEIAAVK"
     gene            1371..1754
                     /gene="rpsI"
                     /locus_tag="VLE22_01125"
     CDS             1371..1754
                     /gene="rpsI"
                     /locus_tag="VLE22_01125"
                     /inference="COORDINATES: protein motif:HMM:NF001099.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HSB13026.1"
                     /translation="MQHIGTGRRKRAVARVFLRLGKGEITVNNRAFEEYFPTEASRSV
                     VRQPLLATETADRFDVLILTDGGGITGQAGAARLGIARALIEFNSELRPKLKELGFLT
                     RDPRSHERKKYGQKGARKRFQFSKR"
     gene            1894..2799
                     /gene="rpsB"
                     /locus_tag="VLE22_01130"
     CDS             1894..2799
                     /gene="rpsB"
                     /locus_tag="VLE22_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSB13027.1"
                     /translation="MPSISMKELLEAGVHFGHQTKRWNPKMKEYIFGERNGIYIIDLQ
                     KTLKLFKDAMRYVAEMAAQGRTVLFVGTKRQAQEAIAEEATRCGMFYVNQRWLGGLLT
                     NMMTVQKSIKRLKELEALAAEGTYAGRAKKEISRLERERKHLDQNLAGIKEMPRLPDL
                     LFVVDSNKESIAVREARKLAIPVVAVVDTNCDPDEVDHPIPGNDDALRAIRLFTNKVA
                     DAIIEGKALATEQDFAAEKIITEEGPGEEIPEYTQYVDPRYAEQIMAEGLSDEDLPSM
                     SLPRKAPGTETPDSALEPKAAEAEK"
     gene            2851..3459
                     /gene="tsf"
                     /locus_tag="VLE22_01135"
     CDS             2851..3459
                     /gene="tsf"
                     /locus_tag="VLE22_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HSB13028.1"
                     /translation="MAEITAQLVKELRERTGAGMMECKNALLEAKGNLSEAETVLRKR
                     GIASAAKKATRATMQGLVGSYIHAGAQLGVLVEVNCESDFVARTNEFGELVHDLAMQI
                     AAADPKYIRREEVPAEVLDKEREIQKARVLAEGKPPQVADKIVEGRLSKFFEETCLYD
                     QPFIKDNSSSIDQLIKTKIAKTGENITVSRFVRFKIGDAQAQ"
     gene            3505..4215
                     /gene="pyrH"
                     /locus_tag="VLE22_01140"
     CDS             3505..4215
                     /gene="pyrH"
                     /locus_tag="VLE22_01140"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HSB13029.1"
                     /translation="MTTYKRILLKLSGEALAAEAGFGIDAERVRSLAAEVAEVAARGV
                     EIGVVVGGGNFFRGVSTVARKMDRVTADHMGMLATVINALAFQDALEAVGAPTRVMTA
                     IEMRQVAEPFIRRRAIRHLEKRRIVIFAAGTSSPYFSTDSAAALRALEIRAEILAKGT
                     RVDGVYDMDPLKHPDATLYRRISYTEVLTQSLKVMDATSIAMCRDNGLPIVVFNLNVA
                     GNIMRMSMGEPVGTLIHP"
     gene            4223..4828
                     /gene="frr"
                     /locus_tag="VLE22_01145"
     CDS             4223..4828
                     /gene="frr"
                     /locus_tag="VLE22_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSB13030.1"
                     /translation="MKPGQQTAATAQAFRTVKDVENYARSRMEKVLSDLQHEMAGIRT
                     GRASVSLFDHVKVDYYGTPTPLNQLATLHVPEPSLITIQPWDVSQIGVIEKAIRSSDL
                     GLNPNNDGKLIRVPIPPLTEERRKELVKHLHGVTEDHRVAVRNIRRDANEAVKKLVKD
                     KLISEDEERRALDEIQKLTDGNTQKLDQASKTKEKEILELK"
     gene            complement(4850..5443)
                     /locus_tag="VLE22_01150"
     CDS             complement(4850..5443)
                     /locus_tag="VLE22_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSB13031.1"
                     /translation="MDTVAAGNFTNGLSTWDLDASNVTDIGLDNRQVAFDGTGVYVAV
                     LDSGLLDSWRRYFPQERIAEEYARSFTGGRFEWTVATQPNKWQHDQNSHGTHVTSTIL
                     GYSLGGAPINGVAPMATVIPVKVLNQNGSGWSSVIAQGLIYVADLKAGPLADYPVVVN
                     MSLGGPALEASAGAPTRPAPASRQRTPHSPLHRRYFG"
     gene            5873..6985
                     /locus_tag="VLE22_01155"
     CDS             5873..6985
                     /locus_tag="VLE22_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSB13032.1"
                     /translation="MTRRDFANRLAWAAAGVNVWSEAALAQRALVRADMPGDTVWLNA
                     NENPEGPGQAALEAMREVMPRSWRYHYQEFGEFYATVSRSEGFGREHVVVGAGSSEVL
                     SIAVGVFTSPSRPLIAAVPTFELPEGVARSLEHPVVRIPLADGYTSDVKRMAAEAEKA
                     GGGLIYLCNPNNPTSTITGKGDLAWLVSNLPANTVLLVDEAYLHFSESPELETGLRYV
                     REGKDVVVARTFSKIYGMAGLRIGFSLSKPELTRQMEPFRNSVISIVSARAAKAALAE
                     AATLIPQRRTKLAKIRASLCAWLRERNLNYLEPHANFVMVDLGREVTSVLPKMYRRGV
                     APGRPFPPLTNMMRVTIGTEAEMEKFKKTFAEVMSV"
     gene            6986..8185
                     /locus_tag="VLE22_01160"
     CDS             6986..8185
                     /locus_tag="VLE22_01160"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSB13033.1"
                     /translation="MHASRSLLMWITIAALTMSAAERKVIVPAGSAKPVGPYSPGIMA
                     GDYLYVSGQGARDAQGQMPATFEEQARLCLQNVKLIVEGAGLTLRHVVYSQVYLSDMN
                     NYGTMDRLWTEYFPDAPPARAVLGVQRMPIDTPIEINAVAVWDLAGRKNVEGGGVLTH
                     DRLYLPGAFGVDQATGQVPADPAAQVQAALDGMKRVLTAAGLDFRHVVFVNPYLTAEM
                     PMGVMNRVYASHFEFGNTPARATIEVASLPHGARIEFTGVAVRDLSQRRAVRPKNMAP
                     SPTASPCVFAGDTLFCSAKSAFIPGPNSGIFAEDVEHQVRMSMRNLLDGLEEAGMDFS
                     HSVATNVYVDDMNEFAKMNRIYALFFPKDAPPTRTTVQPKASVERRRDADGRAPKLEE
                     ISVVAVQ"
     gene            8239..9246
                     /locus_tag="VLE22_01165"
     CDS             8239..9246
                     /locus_tag="VLE22_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSB13034.1"
                     /translation="MFAQTKVWLFLVLVIALALPLWNCAASYHEAAEMYVLVATNIKI
                     PYWQAATAGLNQAAAQLKVKADVVGPEHWDPKAQRDEFRRIVAKKPAGIMVSPADPEL
                     MGPEIDAAIAQGIPVVTVDSDAPKSKRLLYIGTDNYQAGVMGARQAVEQLQGKGNVIV
                     FTMPEQANLNDRLRGYSEVFAGHPQIKIAEVINVKGDPTIAFDRTMEMIEKSPGSADA
                     FVCMEALACEEVAEVLDRKHLTGKVVVAMDTDELTLEWVRKGSIVATVAQKPFTMAFT
                     ALKVLDDLHHHKPASLDKKWAQDPFSPIPSFIDTGASLVTKGNLDEYVRAREAATTKG
                     K"
     gene            complement(9258..10844)
                     /locus_tag="VLE22_01170"
     CDS             complement(9258..10844)
                     /locus_tag="VLE22_01170"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HSB13035.1"
                     /translation="MLRVRIALVLLSVTGGALAQGPPFDLLIRNARIVDGSGNPWFYG
                     DIAIRADRIAAVGALGDAPARTRVEARRMVAAPGFIDIHTHGRGGIFEAPTAPNYIRQ
                     GVTTLLEGQDGSSPLPLRPFLDKLSATPLSVNFGMFTGQGSIRRAVVGLENRRATREE
                     IRKMIELADQAMRDGAFGLSTGLFYVPGNYTPTEEIVEIARAVGALGGIYISHIREEA
                     SQVIQSVQEVIRIAEEAHLPAQVTHHKIVGRANWGRSTETLRLIGQARARGVDVTADQ
                     YPYTATSTGTAALFPQWALEGGNDQLMERLRRPDIRARIKAEIVSRILNDRGAGDPKN
                     VAIASCPFDPTLAGKSLAEITALAGREPTAQNAAETAIELQQKGGCAAIYHALDEGDL
                     ERILRAPFTMIGSDGEIPMFGAGVPHPRSYGTFARVLGRYVRERRILTLEEAVRRMSS
                     FPAARLGLSDRGLLRPGMKADLVVFDPARIRDTATFAQPHQYAEGVLHVFVNGKPVVL
                     NGKVTQERPGEVIYGPARRL"
     gene            11281..12606
                     /gene="larA"
                     /locus_tag="VLE22_01175"
     CDS             11281..12606
                     /gene="larA"
                     /locus_tag="VLE22_01175"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HSB13036.1"
                     /translation="MRVELAFGKTGLELNLPDGPRYRILEARSAEPLTDAAAAIDQAL
                     DTPIGCAPVMEMAKGKGSAAISVCDITRPAPNRQVLPPLLRRLERAGIPRERITILIA
                     TGLHRPATGAEIREIVGPEIAESYRVGNHNARALAEHRYLGATASGTPVYIDERFVSA
                     DLRLSLGFIEPHLMLGYSGGRKLVAPGLAAKETIEVLHSPKFMRDPRSAEGSIDDNPL
                     HRELLEIARMAGHTFIADTALTRDRRIAGVFAGHPERAHRRGVEFVSQVMLETLDEPA
                     DAVITTAAGYPLDLTFYQSIKGITAASHIVKPGGSILLVAACEEGAGAPEFRSMLKEF
                     PSDRGFLDRIDAAPVVIDQWQLEKLALVTRKVRVLFYVPGLPAEYYPTLWGTAYESAA
                     NALDALIGRLPGQPTVAVIPEGPYVLAKTPSEEAGVAVSQASLLGGDSG"
     gene            12823..14553
                     /locus_tag="VLE22_01180"
     CDS             12823..14553
                     /locus_tag="VLE22_01180"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HSB13037.1"
                     /translation="MAGNADERRPKPEVPAEITDYEQLIDDYSHFAPPAEGEVLQGHI
                     LKVTDKEVIVDFGYKSEGLVPIAQFVQLDGSILVHPGDTIDVMIDRGGAEIEGYILLS
                     HEKAVRLRAWENLEKAYRERLMVSGRVLGRMKGGLSIDVGVKAFMPGSQLDVRPVHNL
                     DSFVGQDIPVKIVKLNRRRGNVVVSRKAAIEEEIHSRKSAVMDVLEEGALVRGVVKNL
                     TDYGAFVDLGGIDGLLHVSDISHGRVGHPSEVLHVGDEITVKVLKFDKEKERISLGLK
                     QAQPDPWELVEDRYPVNHRVIGRVVSITDYGAFVELEAGIEGLIHISEMTWSRRMKHP
                     SKVVKVGDQVEAVVLAVQSKERRISLGIKQLEPDPWTTVEARYSVGSVVEGRVRKLTE
                     FGAFIEIEEGIDGLVHVSDLSWTKRVKNPAEILKKGQMVQAAILSIDASNRRLSLGIK
                     QLQPDAWETFFRSHQMGDVVRGRIVRAVNFGVFVELAPGVEALCHNSEIPGAADRHRE
                     EPVLPIGEELDFKIIKLNEADKKIGLSVQAVAEDEERSRLEDYHRQAAAATMTIEEVI
                     NLKGRGDIRS"
     gene            14598..14945
                     /locus_tag="VLE22_01185"
     CDS             14598..14945
                     /locus_tag="VLE22_01185"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSB13038.1"
                     /translation="MTKADLIDEVSRVVEMTRKDSEVIVDAIFESVVRSLRTGEKIEI
                     RGFGSFRTRERRARVGRNPKTGARVEVPPKRIPYFKPSKELKDLVNEGTAEVVSPGVS
                     APEAASAESNPTS"
     gene            14967..15455
                     /locus_tag="VLE22_01190"
     CDS             14967..15455
                     /locus_tag="VLE22_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HSB13039.1"
                     /translation="MDRLWSPWRYRYVSQADAGGPCIFCAKSAGNNDAADYVVHRGHS
                     NFILLNLYPYTTGHLMVAPYLHVATLEEAPEETVIEMMRLTRKAEVALRSVYRPLGLN
                     IGMNIGECAGAGVAGHIHMHVLPRWPADANFMTTVGETRVLPEDLDVTYAKLLNAFRR
                     IL"
     gene            15478..16425
                     /locus_tag="VLE22_01195"
     CDS             15478..16425
                     /locus_tag="VLE22_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HSB13040.1"
                     /translation="MIWRLRSLWLSCLMAACLPAAEPARVIFDTDIGNDIDDVLALAM
                     LHAFESRGDVELLAVTVTKDNRLAGQAASAINTFYGRQAIPVGVVNNGVTRGDGNYNR
                     VIVDRYPHAASFEDAVTVLRKALAAQPDNSVSIVQVGFSTNLAHLLESPGGRDLAAKK
                     VRRLVVMAGDFERPAYREYNIKEDVASAARVFGEWPTEIVASGYEIGERIKYPAKNIE
                     RDFAWTPHHPVAEGYRAYMKMPYDRQTWDLTAVLYGVRPEDGYFDLSDRGTIRVGRGG
                     ETRFDASPDGRHRYLTVNEVQRARILEAFLYLASQPAAR"
     gene            16467..17369
                     /locus_tag="VLE22_01200"
     CDS             16467..17369
                     /locus_tag="VLE22_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB13041.1"
                     /translation="MVHSRVSRRELLTGIAGVPAVLATKSAYKPKLSVQTYVWTQVLG
                     EQKRTLGDGLEEIFAGCKKAGFREMEVGKELLAPDLKSKTAELLKKYKMRLTSVYAAG
                     PMYEAAMAEKTIAEAVETAEAARELGVKMILSNPNPKPNRGRKSDEELAIQARYVTKF
                     GEELQKRGMKLALHHHNPEMAENAREWRHLLKNVDPKLAGICIDVDWVIRGGQDAMTM
                     LREAGSRIVSLHLRNSRHGVWTESLDEGDYDYGSVAAFLKEIGFQGYLIVELAHEKGM
                     TISRPLVENLRRSREFTEKVFGVR"
BASE COUNT         3603 a         4963 c         5688 g         3164 t
ORIGIN      
        1 ggatcgaagt catcgccgcc ggcgacaaca tgaagatttg ttgcggcgac acgggactca
       61 gagacttcta tgtgtgcgga tgcgaagttg ctgacggtga aggggactat ggattagcgt
      121 gccgactaag actaggcaag aagaggtatc gagataaaag catatggttt tggggatttc
      181 ggaatatcca ccgcgcaaac agtttctgag gacaccgatg gacatccgat tttcgaaatg
      241 gccggtaacc gggtgggggc gggcgggagc cttggcacgg actgggcggt ccggcaacgc
      301 agcgcgcgag tatggactgg tcaatcaggg tggaaactga tctgtttgag caccgggaag
      361 ctaagccgca cttcatcaat ccctgttgct ttggtgagga ctttgctggc tggttgaagc
      421 aacaactgtc gccgcttgca ggttccggct attccttttc agagtctatc caggaggact
      481 acggctgggg cttcggggct tcccgtggaa aagaccggtt ttgggtagcc atctcctaca
      541 tgggcgatgg gccgcaagaa cccccagctc aatgggtcgt atcggtaaac catgaccctg
      601 ggctaaaccc gatcaagcgc ttactctaca agcctgatcc cgcagcgttg gcggagctgc
      661 gagatcgagt ccagcgagct cttggagcca atggcgcgat cagaacaatc ggcccggtcg
      721 agacgcactg atccgccccg ttgcgggcgg ttggcgccgg ctagacgaac gacggcgccg
      781 tccgcgccgg ccatgcgaac gcggactcgg tccgtgcacc gccgcccgat ttgacagcga
      841 gcgacaagtc ctgtagccta agaatgttac ggcagtaatc gaagaggaaa cgtctgtcca
      901 tgaataccgt atttccgtcc gggaacgaga tcaagcgcaa ctggttcgtg cttgacgccg
      961 gcgaccaggt actgggtcga gtggcaagca aagcggccat gattctgatg ggcaagcaca
     1021 agccgggcta cgctccgttt ctggacaccg gcgatcacgt cgtggtgatc aacgcgtcca
     1081 agatccggct caccggccgg aaagaacaac agaaagtcta tcgcagtcac tcggggtacc
     1141 cgggtggcct gagggaagtc agcgtcgccg tgatgcgcgc caagcgcccg gcgaagattg
     1201 tggaagaagc ggtcgcgggc atgcttccga aaaccaagct cggtaagcac atgctgaaaa
     1261 agctcaaagt ctacgcgggt gagcggcacc cgcatcaggc tcagaagccc gtcgagatcg
     1321 ccgctgtcaa gtaggcagag gagagaggaa tccgagtggc aggaacgatt gtgcagcata
     1381 ttggaaccgg gcgtagaaag cgcgcagtgg cgcgcgtttt tctgcgtctg ggcaagggcg
     1441 aaatcactgt taataaccgt gcttttgagg agtacttccc cacggaggcg agccggtcgg
     1501 tcgtgcggca gcctctgctg gccaccgaaa ccgcggacag gttcgacgtc ctgattctta
     1561 ccgacggcgg tggcatcacc ggacaggccg gtgcggcgcg tctgggcatc gcgcgggcgc
     1621 tgatcgagtt caactccgag ttacggccga agctcaaaga gctcggcttt ctcacacgtg
     1681 atccgcgaag ccacgagcgc aagaagtacg gccagaaggg cgcgcggaag cggttccagt
     1741 tctcgaaacg ttaatgtccc tggagtcgcg gactcatttc aggcttacca tcggtttgcc
     1801 tgtcccgggc ccgcgggcat ccctgtttta ggattttccg cgcgcaaatt ccgacttggg
     1861 ccgcgcgcga gttgattcga ttaaggaggc agtttgccgt caatttcgat gaaggaattg
     1921 ctcgaagccg gcgttcactt cgggcaccag accaagcgct ggaatccgaa gatgaaagag
     1981 tacatcttcg gcgaacgtaa tgggatctac attatcgacc ttcagaaaac tctgaagctc
     2041 ttcaaagacg ccatgcggta tgtcgccgag atggcggcgc aaggcaggac ggtgctgttc
     2101 gtcggcacca agcgccaggc acaggaggcg atcgcggagg aagcaacgcg ctgcgggatg
     2161 ttctacgtga atcagcgctg gctgggcggg ttgttgacca acatgatgac ggtccagaag
     2221 tcgatcaaac ggctgaagga gctggaagcc ctggccgccg agggaacata cgcgggaagg
     2281 gccaagaagg agatctcccg cctggagcgc gagcgcaaac acctcgacca gaacctggcg
     2341 gggatcaagg aaatgccccg gctgcccgat cttctgttcg tggtcgattc aaacaaggag
     2401 tcgatcgcgg tgcgcgaggc ccgcaagctg gcgattccgg tggtagcggt ggtggacacg
     2461 aactgcgatc cggacgaggt ggatcacccg attcccggca acgacgacgc gctacgggcc
     2521 attcgcctgt ttacgaacaa agtcgctgac gcgatcatcg agggcaaggc tctggcgacc
     2581 gagcaggact tcgccgccga gaagatcatc accgaggaag ggccgggcga ggaaatcccc
     2641 gaatacacgc agtacgtgga cccgcgctac gccgagcaga tcatggccga gggcctctcc
     2701 gacgaggacc tgccttcgat gtcgctgccg cgcaaggcac ccggtacgga aacgccggac
     2761 agcgctctgg agccgaaagc ggccgaagcg gagaaatagc ccgcgccgcg tttcgtctcg
     2821 tttcagattc tcgacgacaa ggaagatcat atggcggaaa ttaccgcgca actggtcaag
     2881 gaactccgcg agcgcaccgg cgcggggatg atggaatgca agaacgctct gctcgaggca
     2941 aagggcaacc tttccgaggc ggagacggtg ctgcgaaagc gcggcatcgc gtcggccgcc
     3001 aagaaggcga cccgcgcgac gatgcagggc ctggttggga gctacatcca cgccggtgcc
     3061 cagctggggg tgctggtgga agtgaactgc gagtccgatt ttgtggcgcg gacgaacgag
     3121 ttcggcgagc tggtacacga cctggcgatg cagatcgccg cagccgatcc gaagtatatt
     3181 cgccgggagg aggtcccggc ggaggtgctc gataaggagc gggaaatcca aaaagcacgc
     3241 gtcctcgccg aggggaagcc gccccaggtg gcggataaga tcgtcgaagg acgcctcagc
     3301 aagttctttg aggagacctg cctgtacgat cagcccttca tcaaggacaa ctcgtccagc
     3361 atcgaccagc tcattaagac caagatcgcg aagaccggcg agaacatcac ggtgtcgcgg
     3421 ttcgtgcgct tcaagatcgg cgacgcgcag gcacaataag cggagggagg ggacgggcga
     3481 ggacgtctat cccccggacc gggcgtgacc acctacaagc ggatcctatt gaagctcagc
     3541 ggcgaagcct tagccgcgga agccggcttc ggcatcgacg cggagcgcgt ccgctctctg
     3601 gcggcggagg tggctgaggt ggctgcgaga ggggttgaga tcggtgtggt cgtcgggggc
     3661 ggcaatttct tccgcggcgt ctccacggtc gcgcggaaga tggaccgcgt gacggcggac
     3721 cacatgggaa tgcttgcgac ggtgatcaac gcgcttgcat ttcaggacgc gctcgaagcg
     3781 gtcggcgcgc ccacgcgggt gatgacggcc atcgagatgc ggcaggtggc cgagccgttt
     3841 atccgccggc gggcgatccg gcacctcgaa aagcggcgaa tcgtcatctt cgcggcggga
     3901 acgtccagcc cttacttttc caccgattcc gcggctgccc ttagggcgct cgagatccgg
     3961 gcggaaattc tggcaaaagg aacccgggtc gacggtgtct acgatatgga cccgctgaaa
     4021 caccccgatg ccaccctcta ccgccggatt tcatataccg aggttctgac acaatcgctg
     4081 aaagtgatgg acgcgacttc gatcgcgatg tgccgtgaca acggcctgcc gatcgtcgtc
     4141 ttcaatctca acgtggcagg caatataatg cgaatgtcga tgggcgagcc cgtcggcacc
     4201 ttgatccatc cgtgaggctt atatgaaacc aggacagcag accgcggcta cggcccaggc
     4261 gtttcggacg gtgaaggatg tcgagaacta tgcgagatcg cgcatggaga aggttttgag
     4321 cgacctccag cacgagatgg cgggtatccg caccggacgc gcctcggtga gcctcttcga
     4381 tcatgtgaag gtggactact acggcacacc cacgcccctt aaccagctgg caaccctgca
     4441 cgtgccggag ccctcgctga ttacgatcca gccttgggac gtctcgcaga tcggggtgat
     4501 cgagaaagcg atccgcagtt cggatctcgg tctcaatccg aacaacgatg gaaagctcat
     4561 ccgcgttccg atcccgccgc tgaccgaaga gaggcgcaaa gaactggtga agcacctgca
     4621 cggggtgacg gaagatcacc gtgtggcggt acggaatatc cggcgggatg ccaacgaggc
     4681 ggtcaagaag ctggtcaagg acaagctgat cagcgaagac gaggagcgcc gcgcgctgga
     4741 cgagatccag aagctgaccg acggaaacac ccagaagctc gatcaggcct ccaagaccaa
     4801 agaaaaagaa atcctggaac tgaagtaggg acgcgccggt ggcgcgcccc tagccgaagt
     4861 acctacggtg tagcggcgag tgcggtgtcc gctgtcgcga ggccggtgcc ggtcgtgtcg
     4921 gcgccccagc agatgcttcg agagccggcc cacccaggct catgtttaca accacgggat
     4981 aatcggccag cggtccggcc ttcagatcag cgacgtagat gagtccctgt gcgatcacgg
     5041 acgaccagcc actaccgttt tgattcaata cctttactgg gatgacggtg gccatgggcg
     5101 caacgccgtt aatcggggcg ccaccaagac tatagccgag gatggtactg gtgacgtggg
     5161 tgccgtgcga attctggtcg tgctgccatt tgttcggctg ggtggcaacg gtccactcga
     5221 atcgccctcc ggtaaagctg cgggcgtact cctcggcgat gcgctcctgg ggaaaatacc
     5281 gccgccagga atcgagcagg ccggagtcca gtaccgccac atagaccccc gtaccgtcga
     5341 aagcgacctg ccggttgtcg agaccgatat cagtgacgtt gctggcgtcg aggtcccaag
     5401 tgctgagacc gtttgtaaag ttcccggcgg ccaccgtatc caccggggcg cggttctctc
     5461 ggcatccggg ttggccacgg ccacgtaggg cagggcagcg atcacggcta actgttccgc
     5521 gggcgcctgc atcagcacgg cgttgatctc tggaatgctg tcccgcacct ttccgtactt
     5581 gccgaggtcg gccagcactg gagccgccac cggcaccttc agcacgacgt tgatgccgac
     5641 gacattcttg ccagacggtg ctgcgaacag cagcggcgag acgccgacca caaaaatcac
     5701 gacaagtatc gagagaagac ttctcttcat cgttttctcc tcattcgcac aattcgagta
     5761 tgagacctta aaatcccggt tcaatttaca ggcttactgc gcgcttcgca aggcatgacc
     5821 gttccggtac ctgcggtatc atacgtccaa caacgcgagg aggtggttca ggatgacgcg
     5881 gagagacttt gcgaaccgct tggcttgggc cgcggcggga gtgaatgtgt ggagcgaggc
     5941 agcgctcgcc cagcgagccc ttgtgcgggc cgacatgccg ggcgatacgg tctggctgaa
     6001 cgccaatgag aacccggagg gtccggggca ggctgcgctc gaggcgatgc gagaggtcat
     6061 gccgcggtcc tggcgctacc actaccagga attcggcgag ttctatgcga cggtctcgcg
     6121 gagcgaagga ttcggccggg agcacgtggt tgtcggcgcc gggtcaagcg aggttctgag
     6181 catcgcggtt ggcgtgttca catcgccttc gcgcccgctc atcgccgcag ttccgacatt
     6241 cgaattgccc gagggcgtcg cccgctctct tgagcatcct gtcgttcgca ttccgttggc
     6301 tgacggctac acgtcggacg tgaaacgcat ggctgcggaa gcggagaagg ctggcggcgg
     6361 cctgatctat ctctgcaatc cgaacaatcc gacctccacg ataacgggaa agggcgacct
     6421 cgcctggctg gtctccaacc tcccagccaa cacggtgctg ctggtcgatg aggcgtacct
     6481 ccatttcagc gaaagccccg agctggagac cggactccgg tacgtacgcg agggcaagga
     6541 tgtcgtggtg gcgcggacct tttcgaaaat ctacggaatg gcgggcctcc gaatcgggtt
     6601 ctcgctgtca aagcctgaat tgacgcggca gatggagcca ttccggaaca gcgtcatttc
     6661 tatcgtcagt gcgcgggccg cgaaagcggc tctcgcagag gcggcaactc tgatcccgca
     6721 aaggcgcacg aagctggcga agatccgcgc gtcgctttgc gcctggttgc gcgagcgtaa
     6781 tctcaactac ctagagccgc acgccaactt cgtgatggtc gacctggggc gcgaggtgac
     6841 gtcggttctc cccaaaatgt atcgccgcgg tgtggcgcct ggacggccgt tcccgccact
     6901 cacaaacatg atgcgtgtca caatcggtac ggaagcggaa atggaaaaat tcaagaaaac
     6961 atttgcggag gtcatgagcg tctaaatgca tgcgtcccgc tctcttctca tgtggattac
     7021 gattgcggct ctgacgatga gcgccgccga gcgaaaagtg attgtccccg ccggttctgc
     7081 aaaacctgtg ggtccataca gccccggtat catggccggc gactatctgt acgtctccgg
     7141 ccagggcgct cgggacgctc aggggcagat gccagcgacg ttcgaggaac aggctcggct
     7201 ctgcctgcag aatgtgaaac tgattgtcga gggcgcaggg ctgactctcc gccacgttgt
     7261 ttattcccag gtctatctgt ccgatatgaa caactacggg accatggacc gtctatggac
     7321 ggagtacttc ccggatgcgc cgccggcgcg ggctgtgctc ggtgttcagc gtatgccgat
     7381 cgacacgccg atcgagatca acgccgtcgc agtgtgggat ctggccggaa ggaagaacgt
     7441 cgaaggcgga ggcgtgctga cacatgaccg tttgtatctg ccgggggcct tcggtgtgga
     7501 ccaggccacc ggccaggtcc ccgctgatcc ggccgcgcag gtgcaggccg cgctcgacgg
     7561 catgaagcgg gtgctgacgg cggcaggtct ggacttccgg cacgtcgtct tcgtgaatcc
     7621 gtacctcacc gccgaaatgc cgatgggggt catgaatcgt gtttacgcga gtcatttcga
     7681 gttcggcaac actccggcac gggcgacgat tgaggtggcg agtttgccgc acggggcgcg
     7741 gatcgaattc accggcgtgg ccgtgcgtga tctcagccaa aggcgggccg tgcgcccgaa
     7801 gaacatggcg ccgagcccca cagcgagccc gtgcgtgttc gccggcgaca ccttgttctg
     7861 ctcggcgaaa tcggcgttta ttcccggtcc caacagcggc attttcgcgg aggatgtcga
     7921 acaccaggtt cgaatgtcga tgcgaaacct tctcgacggt cttgaggagg caggcatgga
     7981 cttctcgcac tccgtggcga caaacgtcta tgtcgacgat atgaatgagt tcgcaaagat
     8041 gaaccggatc tacgcgcttt tctttcccaa ggacgcgccg ccgacgcgaa ccaccgtgca
     8101 gccgaaggcg tccgtggaac gccggcgcga cgctgatggc cgggctccga agctggaaga
     8161 gatttccgtg gtggctgtgc agtgaggcgg aactccgagc cgtaaagggc gttctatact
     8221 gcagcttaga ggaagaacat gttcgcgcag accaaagtgt ggttgtttct cgttctcgta
     8281 atcgccctgg ctctgccgct ctggaattgc gcggcgtctt accatgaggc cgccgaaatg
     8341 tacgtcttgg tggcgacgaa catcaagatt ccgtactggc aggccgccac ggcaggtcta
     8401 aaccaggcgg cggctcaact caaagtgaag gcggatgtcg tggggcctga acactgggat
     8461 cccaaagcgc aacgcgatga atttcgccgg atcgtcgcaa agaaaccggc cggcatcatg
     8521 gtttcaccgg cagaccccga gttaatgggg cctgagatcg acgccgccat cgctcaagga
     8581 atccccgtgg tgaccgtcga ctcggacgcc ccaaaaagca agcggctgct gtacatcggt
     8641 accgacaact accaggccgg cgtcatgggc gcccggcagg cagtcgagca acttcagggc
     8701 aagggtaatg tcatcgtctt caccatgccc gagcaggcga atcttaacga ccggctgcga
     8761 ggctacagcg aggtcttcgc tggccacccg cagatcaaga tcgccgaggt gatcaacgtc
     8821 aaaggcgatc cgacgatcgc cttcgaccgc acaatggaga tgatcgagaa gtcgcccggc
     8881 agcgccgacg cgtttgtgtg catggaggcc ttggcctgtg aggaagtggc ggaggttctc
     8941 gaccgcaagc atctgaccgg caaggtcgtc gtggcgatgg ataccgacga actgacgctg
     9001 gagtgggtcc gcaaaggctc cattgtggcg acggtcgcgc agaagccgtt caccatggct
     9061 ttcaccgcct tgaaggttct ggacgatctg caccaccaca aaccagcttc gcttgacaag
     9121 aaatgggcgc aggatccgtt ctcgcccatc ccatccttca tcgacaccgg agccagcctt
     9181 gtgaccaagg gcaatctcga tgagtacgtg agggctcgcg aggcggcgac cacgaagggc
     9241 aagtgaaacg cggcggatta tagacggcgc gcgggcccat agattacctc gcccggccgt
     9301 tcctgagtga ccttgccgtt cagcacgacc ggctttccat tcacgaacac gtgcagtacg
     9361 ccttccgcat attggtgcgg ttgcgcgaat gtcgccgtat cccgaattct cgcaggatca
     9421 aaaactacca ggtcggcctt catgccgggc cggagcaacc cgcggtctga gagtccgagc
     9481 cgcgctgctg gaaatgacga cattcgccgg acggcctcct cgagcgtcag aatcctcctc
     9541 tcccggacgt agcgtcccag gacgcgggcg aacgtaccgt agctgcgggg atgaggaact
     9601 ccggcgccga acatcgggat ctcgccgtcc gagccgatca tcgtgaacgg agcccggagg
     9661 atgcgttcga ggtcaccttc atcgagcgcg tggtagatgg ccgcgcagcc tcctttttgc
     9721 tgaagttcga tggcagtctc ggccgcattc tgggctgtcg gttcgcgtcc tgctaaggcg
     9781 gtgatctccg ccaagctctt gccggccagg gtgggatcga aaggacacga ggcgatggcc
     9841 acgttcttcg ggtctccggc tcccctgtcg ttcaagatcc gggagacgat ctcggccttg
     9901 atgcgggctc ggatgtcggg gcggcggagg cgttccatga gctgatcgtt gccgccctcg
     9961 agggcccatt gggggaaaag cgccgccgtg ccggtgctgg tcgccgtgta agggtactgg
    10021 tcggcggtca cgtcgacacc acgagcgcga gcctgtccga tgaggcgaag ggtctcggtg
    10081 ctacggcccc agttggcccg gccgacaatc ttgtggtggg tcacctgagc cggcagatgc
    10141 gcttcctcgg caatccgaat cacctcctgg acgctctgga tcacctggga ggcctcctcg
    10201 cggatgtgcg agatgtaaat tcctccgagt gctccgacag cacgagcaat ctcaacgatc
    10261 tcctcagtcg gcgtgtagtt acccgggacg tagaacaagc cggtcgaaag cccgaaggcg
    10321 ccatcgcgca tggcctggtc ggcgagttct atcatttttc ggatttcttc ccgggtggcc
    10381 cttctgtttt cgagacccac caccgcacgc cggatcgatc cctggccggt gaacattccg
    10441 aagttgaccg acaagggcgt ggcggatagt ttgtcgagaa acggacggag gggaagggga
    10501 gaactgccat cctggccttc cagtagcgtc gtcactccct ggcggatgta gttcggggca
    10561 gtgggagcct cgaagatgcc gccgcggccg tgagtgtgga tatcgatgaa gccgggtgcg
    10621 gccaccatcc ggcgggcttc cacgcgggtg cgtgccggag cgtcaccgag agcgccgaca
    10681 gcggcgatac ggtcggcgcg gatagcgatg tcaccgtaaa accacgggtt tccggagcca
    10741 tcgacaatcc ttgcgtttcg gattaacagg tcgaacggcg gcccctgggc aagcgcgccg
    10801 ccggtgacag acaagaggac caaggcaatg cggacccgca acatgcaggc cattttacac
    10861 ggtgatgtgt gtaggaattc cgcggacgcg gggcggaatg aaactcagcg ggggctcttc
    10921 gggctcgagg agagcatggc gaagaacagc ccaagcacta acaaagccgt gattgcggaa
    10981 gcgacccaca taaaatcgtc tccctgacgc gataaatcgg ttgcaattcc gattccaaat
    11041 agatcaaatc ataaagcatt cccgccggac cgcctagcgt ctcatcggtg gcggtgagga
    11101 gatcatggag agaaacaggc cgaggacaaa cagtgcgccg acgaccaccg gaatccagaa
    11161 ctccatagcc acaactcctc tcttcctttg gtgcaacgcg acggcaaaaa gtttcaacct
    11221 gcggcgggtt cggcgcgaat tagccgcaac agtggaccat cctgtgctac ggtttttcat
    11281 atgcgggtgg aactggcgtt cgggaagacg ggacttgagc tcaacttgcc ggacggtccc
    11341 cggtaccgga tactggaagc gcgttccgca gaaccgctga cggatgcggc cgcggccatc
    11401 gatcaggcgc tggacacgcc catcggttgc gcgccggtca tggagatggc gaagggaaag
    11461 ggaagcgccg ccatctccgt ctgtgacatc acgcggccgg cgccgaatcg acaggtgctt
    11521 ccgccgctcc tgcgccggct cgaacgggca ggcatcccgc gtgagcggat cacgattctt
    11581 atcgccaccg gcctgcaccg cccggccacc ggcgccgaaa tccgggagat cgtggggccg
    11641 gaaatcgcgg agtcttaccg cgtcgggaat cacaatgccc gggctcttgc cgagcaccgc
    11701 tatttgggcg ccaccgcctc cggtacgccc gtttacatcg atgagcggtt cgtgtcggcg
    11761 gatctgcggc tttcgttggg gttcattgag ccacacctga tgcttgggta ctcgggcgga
    11821 cggaagctcg tagctcccgg tctggcggcg aaagaaacca tcgaggtgct gcacagcccg
    11881 aagttcatgc gggatccgag gtcggccgaa gggtcgattg acgataaccc attgcaccgt
    11941 gaactgcttg agatcgcccg aatggccggg cacacgttta tcgccgatac cgccctaacg
    12001 cgggaccgca ggatcgcggg cgtattcgcc ggacaccccg aacgggcaca ccgtcggggc
    12061 gtggaattcg tttcccaggt gatgctggag accctggacg agccggccga cgcggtgatt
    12121 acgacagcgg cgggatatcc gctcgacctg acgttctacc agtccatcaa ggggattaca
    12181 gccgcttcgc acatcgtgaa gccgggcggc tccatattgc tcgtggcagc ctgcgaagag
    12241 ggcgccggcg cgccggagtt ccgatcgatg ctcaaggaat tcccatccga tcgcgggttt
    12301 ctggatagga tcgacgccgc gccggtggtg atcgaccagt ggcagttgga gaaacttgca
    12361 ctggtaacgc gcaaagtacg ggttttgttc tatgtgccgg gcttgccggc ggaatattat
    12421 ccgaccttgt ggggaaccgc gtacgaatcg gctgccaacg ctctggacgc tttgatcggg
    12481 cggttacccg gacaaccaac ggtcgccgtg atccccgaag gaccctacgt gttggcgaag
    12541 acgccgtcgg aggaggcggg agttgcagtc tcgcaagcgt ctctattggg aggagattcg
    12601 ggttgacaag gcatatccgc ttcgctatag tttgtaaggt attgatctgt caacggtttg
    12661 tcgacggagc acagcgtggg ctcaagctct ttggacgtcg gagggggcag gcgtcctgtt
    12721 gggggtcact gttgtccaaa gcggcccctt tgaaacccaa tgtaagttcc cccgggttct
    12781 tgacctgact gttgatggtg tgtttgacct ggagacttgg tcatggcagg caacgcggat
    12841 gagcggcggc cgaaacctga ggttcccgcc gaaataaccg attacgagca gctcatcgat
    12901 gactacagtc actttgcgcc tcccgccgaa ggtgaggtgc tccagggtca catcctcaaa
    12961 gtcaccgaca aagaagtgat cgtcgatttc gggtacaagt cggaggggct cgtacccatt
    13021 gctcagttcg tccagctgga cggctcgatc ctggttcacc ccggcgacac catcgacgtg
    13081 atgatcgacc gcggcggcgc cgagatcgag ggctacatcc tgctgtcgca cgagaaagca
    13141 gtcaggcttc gggcgtggga gaaccttgag aaagcgtatc gcgagcggct gatggtttcc
    13201 ggccgggtcc tcgggcggat gaagggcggg ctctcgatcg acgttggagt caaggcgttc
    13261 atgccgggat cgcaactcga tgtgcggccg gtgcacaatc tcgacagctt cgtagggcag
    13321 gacatccccg tcaagatcgt gaagctcaac cgccggcgcg gcaacgtggt ggtttcccgc
    13381 aaggcggcga tcgaggagga gatccacagc cggaagtccg cggtcatgga cgtcctcgaa
    13441 gagggcgcgc tggtgcgcgg tgtggtaaag aacctgaccg actacggcgc gttcgtcgac
    13501 ctcggcggca tcgatggact gctgcacgtg agcgacatct cgcacggtcg tgtggggcac
    13561 ccgtcggagg tcttgcacgt cggcgacgag atcaccgtca aggttttgaa gttcgacaag
    13621 gagaaggagc ggatctcact gggactcaag caggcccagc ctgacccttg ggaactcgtc
    13681 gaggatcggt accccgtaaa ccacagggtg atcggccgcg tggtcagtat caccgactac
    13741 ggggcatttg tcgagctcga ggccggcatc gagggcctga ttcacatctc cgaaatgacg
    13801 tggtcgcggc gtatgaagca cccgtcgaag gtcgtgaaag ttggcgatca ggtggaggcc
    13861 gtggtgctgg cggtgcagtc caaggagcgg agaatctcgc tcggcatcaa gcagcttgaa
    13921 cctgatccgt ggacgacggt ggaggcgcgc tatagcgtcg ggtccgtcgt cgagggcagg
    13981 gtgaggaagc tcacggaatt cggcgcgttc attgaaatcg aggaaggcat cgacggtctg
    14041 gtgcatgtgt ccgacctttc gtggaccaag cgggtcaaga acccggcgga gatcctgaag
    14101 aaaggtcaga tggtccaggc tgcgattctg agtatcgatg cgtccaaccg gcggctttcg
    14161 ctcggcatca agcagcttca acccgatgcg tgggagacgt tcttccggtc tcatcagatg
    14221 ggcgacgtag tgcgcggccg catcgtgcgg gcagtcaact tcggtgtgtt cgtggaactg
    14281 gcgccgggcg tcgaggcgct atgccacaac tcggagattc cgggggcggc ggatcgccat
    14341 cgtgaggagc ccgtgcttcc catcggcgaa gaactggact tcaaaatcat caaactcaac
    14401 gaagctgaca agaagattgg gcttagcgtt caggcagtcg cggaggatga ggagagaagc
    14461 cgcctggagg actatcaccg gcaggcagcg gccgccacga tgacgattga ggaagtgatt
    14521 aatctgaaag gtcggggcga catccgctcg tagacgcggc ctcaatggtt tcacgagaac
    14581 agtaggagag gagaaagatg acgaaggcgg atctgattga tgaagtctcg agggttgtgg
    14641 agatgacccg caaagactcg gaagtaattg tggacgcgat tttcgagagc gtggtacgct
    14701 ccctgcgcac cggggaaaag atcgagatcc gcggcttcgg cagcttccga acacgggagc
    14761 gccgcgcgag ggtcggtcgt aatccgaaga ccggtgcgcg agtcgaagtt ccgcccaagc
    14821 ggatcccgta tttcaagccg agcaaggaac tgaaggactt ggtgaacgaa ggcacggcag
    14881 aagtagtgtc gcccggtgtt tcagccccgg aggcggcgtc cgccgaatcc aacccgacct
    14941 catagacggc tccttttgac aacagcatgg accgtctttg gagcccatgg cgctatcggt
    15001 acgtcagtca ggccgacgca ggcggcccat gcatcttttg tgcgaagagc gcggggaaca
    15061 acgatgcagc ggactacgtg gttcaccgcg ggcacagcaa tttcatcctt ctcaaccttt
    15121 acccctacac gaccggtcac ctcatggtcg ccccgtacct tcacgtcgcg acactggaag
    15181 aagcgcctga ggagacggtg atcgagatga tgaggctgac gcggaaggcc gaggtcgccc
    15241 ttcggtcggt ctaccggccc ctcggtctga atatcggaat gaacatcggg gaatgcgctg
    15301 gagcgggcgt cgccgggcat atccacatgc acgtgctgcc gcggtggccc gccgatgcga
    15361 acttcatgac aacggttggc gagacacggg tgctgccgga agacctcgac gtaacctacg
    15421 caaagctgct caacgcgttt cgcagaattc tttagccttc gctactttgg agattccatg
    15481 atctggcggc ttcgttcgct gtggctctcg tgcctgatgg cggcgtgtct cccggcggcc
    15541 gagccggcgc gggtgatctt cgacaccgac attggcaacg acatagacga cgtgctggcg
    15601 ctggcgatgc tgcacgcttt tgagtcacgc ggcgacgtgg aactgctggc ggtcacggtg
    15661 accaaagaca accgcctggc gggacaggcg gcgagcgcga tcaacacatt ttacggacgg
    15721 caagccattc cggtgggcgt ggtgaataac ggcgtcacgc gtggggacgg aaactataac
    15781 cgagtgatcg tagaccgcta cccgcacgca gcgagtttcg aggacgccgt aacggttctc
    15841 cgaaaagcgt tggcggcgca gccggacaac tcggtgtcga tcgtgcaggt cgggttctca
    15901 acgaacctgg cgcacttgct tgaatctccg ggcggaaggg atctggcggc gaagaaagtc
    15961 aggcggctcg tcgtcatggc gggagacttc gagaggccgg cctaccggga gtacaacatc
    16021 aaggaggacg tcgcgtcggc cgcgagggtg ttcggcgagt ggccgacgga gatcgttgcc
    16081 agcggctacg agatcgggga acggatcaag taccctgcga agaacatcga gcgggacttc
    16141 gcctggacgc cgcatcaccc ggttgcggaa ggataccggg cttacatgaa gatgccgtac
    16201 gatcgccaga cgtgggacct gaccgctgtg ctttacggtg tgcgtcccga ggacggatac
    16261 ttcgatctgt cggaccgagg tacgattcga gtcggacgcg gcggagaaac gcggtttgac
    16321 gcatctccgg acggcagaca ccggtacttg acagtgaacg aagtccagcg ggcgcgcatt
    16381 ctggaggcgt ttctttatct ggcctcacaa cccgcggctc gatagtgctc gcggcgcgcg
    16441 cgttcaagtt cgttagggag ggaatcatgg tgcatagcag ggtgagtcgc agagagttgt
    16501 tgacgggtat cgcgggtgtg ccggcggtgc tggcgacgaa atcggcctat aagccgaagc
    16561 tgtccgtgca gacctatgtc tggacgcagg tgctgggcga gcagaaaaga accctgggtg
    16621 acggcctgga ggagatcttc gccgggtgca aaaaggcggg ctttcgggaa atggaggtag
    16681 gcaaggaact gctggccccc gacctcaagt cgaagacagc ggagctcctg aagaagtaca
    16741 agatgcggct taccagcgtt tacgcggcag gaccgatgta cgaggccgcg atggccgaaa
    16801 aaacgatcgc cgaggccgtc gagacggcgg aggccgcgcg tgagcttggc gtcaagatga
    16861 ttctctcgaa tccgaacccg aaaccaaacc ggggacggaa gtccgacgag gaactcgcca
    16921 tccaggctcg ctacgtcact aagttcggtg aggaattgca gaaacgcggg atgaaactcg
    16981 ccctccacca ccacaatccg gagatggccg agaacgcccg ggagtggcgt cacctgttga
    17041 agaacgtcga cccaaagctg gctgggatct gcattgacgt cgactgggtg atccgcggcg
    17101 gccaggacgc gatgactatg ttgcgagagg ctgggtccag gatcgtcagt ttgcacctga
    17161 ggaactcccg gcacggtgtc tggaccgaga gcctcgacga gggggactac gactacggtt
    17221 ccgtggccgc gtttctgaaa gagatcggat tccagggcta tctcatcgtg gaactcgctc
    17281 atgaaaaggg gatgacgatc agccgcccgc tcgttgagaa tcttcgccgc agccgggagt
    17341 tcaccgagaa ggtgttcggc gtccggtagt actgcagacc atcgttttcc gtggcctgca
    17401 agagcggcag acgactaa
//
LOCUS       DATIFQ010000030         9396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1923694, whole genome shotgun sequence.
ACCESSION   DATIFQ010000030 DATIFQ010000000
VERSION     DATIFQ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923694"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VLE22_01205"
     CDS             complement(<1..184)
                     /locus_tag="VLE22_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13042.1"
                     /translation="MTRHLTRRGFTRAAATLVAAPAAAFSRGPEFPLVDYHVHIEREM
                     TIERALKIAVERGVKIG"
     gene            230..1849
                     /locus_tag="VLE22_01210"
     CDS             230..1849
                     /locus_tag="VLE22_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HSB13043.1"
                     /translation="MNRLPRQFTPAVCAACVVLASCAGTSRVTDRTFTAADLYGGLEA
                     TRVLLKDDGLVLDTRNWTAESRGIIVTDVIDLAGAGVLAEEAVAREFRVAVNAETPAG
                     SAVNVEVRTGGTYFQEPKTWSDWRAASGAVEPQGRYAQVRLILTTPDPQSVPSVTGLK
                     LSCRIQAVARKQKIVVVSENVQKIVRSPVSFSYERPEQPDLVWLRRTFRLDDVIAGQK
                     TEFGKLRALMHWVATRPNKRPGPWEAKNEPYPWHVRRVMAADGRGAIYGHCMSYCEVM
                     IAAASALGWHGRHWAIHGVRDTSHEVPEFWIDELGKWVFFDPSLDTYYADPETRAPLN
                     LLEMHDLYLKTVLRPGEVQKMGRTHNEDRLKLLRGRHSVLCITGDYAYGKPTKWDWQW
                     DHGYMSAGWMQLTPRNDWHSRPEPAFRWFGQGPEGYDGFPLYLDAQTPLPSKDASLWY
                     TRKRDLWWTLNQASLRLVRTGERTILVECGNSQPFFRRYVARIGDAVWKPVDARFTWE
                     LVPGDNRIEVVSEDASGRRAIMSSAVVRLER"
     gene            2301..2645
                     /locus_tag="VLE22_01215"
     CDS             2301..2645
                     /locus_tag="VLE22_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB13044.1"
                     /translation="MKLVLSLLGTIAFATACLAEDNPGKAIFDRTCKTCHGPDGKGDA
                     AADKFYQIRIPRLNSAYVQQKSDEELKEIITKGRRKMKPVQPGQPIAEHNLKSEWTDS
                     VISYVRTLKKKK"
     gene            complement(2725..6003)
                     /locus_tag="VLE22_01220"
     CDS             complement(2725..6003)
                     /locus_tag="VLE22_01220"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HSB13045.1"
                     /translation="MRTVCAVLALCASCGWSATVTVSEGRIDIPAYEHVGRETQPPLF
                     AGSTVGGMYPLPPFILPLKGEPKPRTYGALFVENEYLKLTYLPEFGGRLFSLYDKVRK
                     REVFYRNDVVKPANYNSKGSFPLFGIELTGAFDTHMLTLYGEPFWSHRVVRHADGSVS
                     LVLGTTDPVYRMKVNFSARLYPALAVVQMSVFCYNTRDGRRPHMFWISGSLRATEQTR
                     FVYPMTRTIGHTTSEVGDWPVFNGIDYSWDRNNKNMLGVFGIDIYDDFQGAYHEDLDY
                     GVFRYADRRVVQGMKMWTFGYSDRATYLERAYTDNAGPYIEVQSGRYVWDGYYEWLSP
                     HKVESWSEWWFPVAGIGGLTTTSRDVALHLDVKPDPSGRNSTVTVGLSANRPMPGARI
                     TVTAKSGRILSAVADLAPETPFRHAAGGIASDENGLRGMVVTVADAEGSEVLTYHRPD
                     ESPGRKEYTPFTRPLERPAKEPQEMSIEELALAAEVKYKQLNAAGAAELLNLAIERDP
                     GYSRAHLMFGIDHFNSGRYRTAAEHLEKAIDRDPYLFEAHYYLAMSRLALGQEKAGER
                     SLYFIPPDSAYFANREYHLGRLALLRGERDKAAEHLERAVRSNGYYLDALLLLALMDR
                     EQGARERALARLAEVEAIDPTNQWAAVERFFLARGAETRKTLCELMGGQSQEGLEVSQ
                     FYRRLGRWKEAVEVLMLVEQENHDPWGTPSEFYYTLGHCLKRAGAEAASAEYLKKAQA
                     AAANIDRFPYREETEAVLREAAERDPNDAVARFYLACLLYHQGRTDEAIRQWETAAGI
                     SPSDFRTRRALGLAYEEQGRGVEKAAVQLEKAIAIDPGHVRTLNDLSAIYAKSGRFDE
                     QRALLERALSRSPEDDDLAEGLLLANLAKGRYSEAEKLATGRKFGPRHRTYGLRDKWR
                     FLKYGMGAAAFREGRYDEALRQFEAALAPPVSLGVDDFGFQSVPRAHYYIGRTLEALG
                     RRDEARAAYEKAASGYEHLSGDWDSWNAENVHMALALNRLGKTAAASRILGEMEGFAR
                     SQLDSGRAMRRASSRYLLALVLKHKGDQAEAHRLLDEALQIQPDLLGARLELRGDVVD
                     RPGE"
     gene            complement(6154..6435)
                     /locus_tag="VLE22_01225"
     CDS             complement(6154..6435)
                     /locus_tag="VLE22_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13046.1"
                     /translation="MKTAESSKARLERGFHILQRILLRRALEGSTMIGWGVERAIVDR
                     ELQELEDDLRQHPVVPPMAGWIIRDNIPRRGSIPNRLPRRTGHPTSEDA"
     gene            6698..7276
                     /locus_tag="VLE22_01230"
     CDS             6698..7276
                     /locus_tag="VLE22_01230"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HSB13047.1"
                     /translation="MKILTVLVFVSTSVLFAQGTPADRPMGPRPLRGPVATDEVKAYL
                     NLEQAQVDQLVQLRKDERDEMKAVFEKITENRKALREALQSTSPDASTLGNLLIDMQK
                     LRKQIRETNENYHTKALAVLKEEQKSKLAELEAAAKLRPVIGQAHGLNLLLPPAQEAR
                     DEVMVGGEGAGFGPMGMGMRMRRFGAVGRPRR"
     gene            complement(7606..8280)
                     /locus_tag="VLE22_01235"
     CDS             complement(7606..8280)
                     /locus_tag="VLE22_01235"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSB13048.1"
                     /translation="MVILLFGPPGCGKGTQAVSMARSMGVPSISTGEIFRAECKAGTK
                     IGKLACEILRSGGLVSDEIVNQMVAGRLSQDDCRNGFILDGYPRTKAQAEFLDRLLEE
                     QRLPAPTVIYLDVPDSVLVARITSRRQCPTCSAIYNLLHQPPRHAGVCDNDGTPLIQR
                     EDDNEQTILARLKAYHEATGPLVDFYATRDFHRIEGDRTPAEIHAEIEQVIEPALAHA
                     GEPRRG"
     gene            8618..8869
                     /locus_tag="VLE22_01240"
     CDS             8618..8869
                     /locus_tag="VLE22_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HSB13049.1"
                     /translation="MAYVIVDTCTKDKLCIEACPVDCIHPKEDEPKFEGVPQLFIQPE
                     ECIDCGACVPVCPTNSIYVLDELPANLAQFAEKNAAYYA"
     gene            complement(8952..9200)
                     /locus_tag="VLE22_01245"
     CDS             complement(8952..9200)
                     /locus_tag="VLE22_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13050.1"
                     /translation="MNNEVSIETLAHEIYRVVGQQQGKRYLKAGDLTQMMMERFGERC
                     SKDNCRRAIHQLIDSGRCVYSYFGASYITLPHKAKKQL"
BASE COUNT         1788 a         2987 c         2698 g         1923 t
ORIGIN      
        1 cgccgatttt gactcctcgc tccactgcga tcttgagcgc cctttcgatg gtcatctcgc
       61 gctcgatatg gacgtggtag tctaccagcg gaaattcggg tccgcgggaa aatgccgccg
      121 ccggcgccgc taccagcgtt gccgcagctc gtgtgaaacc tctgcgtgtc aggtgcctgg
      181 tcatgagaag tttccggagt gtaccacagg cgtccctggg tgtgaaaaaa tgaaccggtt
      241 gccgcgacaa ttcacacccg ccgtctgcgc cgcgtgcgtg gtcctcgcct cttgcgccgg
      301 gacctcacgc gttaccgatc gaaccttcac cgcggctgac ctctatggcg gcctcgaagc
      361 cacccgggtc ctgctgaagg atgacggtct tgtcctcgat actcgcaact ggacagccga
      421 aagccgtggc atcatcgtga ccgatgtcat cgacctcgcc ggtgcgggcg tgctggccga
      481 ggaggctgtc gcaagggagt tccgcgtggc agtcaatgcc gaaactccgg ccggctccgc
      541 ggtaaacgtg gaggttcgca cgggcggcac gtacttccaa gagcccaaga cgtggagcga
      601 ctggcgtgct gcaagcggcg cggtcgagcc gcagggccgt tatgctcagg tccgtctgat
      661 tcttacaaca cctgatccgc agtccgtgcc aagcgtgaca ggattgaaac tctcctgccg
      721 gattcaagcc gttgcgcgga agcagaagat cgtagtggtc tcggagaacg ttcaaaagat
      781 cgtccgttca cccgtctcct ttagctacga gcgacccgag cagccggatc tagtttggct
      841 ccggaggaca ttccgcctgg acgatgtcat cgccggtcag aagacggagt tcggaaaact
      901 gcgcgccctg atgcactggg tggcaacccg cccgaacaag cgtccgggcc cgtgggaggc
      961 gaagaacgag ccctatcctt ggcacgtccg gcgcgtcatg gccgctgacg gccggggcgc
     1021 catctacggg cattgcatga gctactgcga ggtgatgatc gccgccgctt ccgcgctcgg
     1081 ctggcacggg cgacattggg ccatccacgg tgtccgcgat accagtcacg aggtgcccga
     1141 gttctggatc gacgaactcg gcaaatgggt cttcttcgat ccatcgcttg acacgtacta
     1201 cgccgatccg gaaacgcgcg ctcctctgaa cctgctcgag atgcacgacc tctacctgaa
     1261 aaccgtcctc cgcccgggcg aggtccagaa aatggggcgt acccacaacg aagaccggtt
     1321 gaagcttctg cggggcaggc attcggttct ctgcattacc ggcgactacg cctacgggaa
     1381 acctaccaag tgggactggc aatgggacca cggctacatg agcgccgggt ggatgcagtt
     1441 gaccccgagg aacgactggc attcccgccc cgagcccgcc tttcgctggt ttggtcaggg
     1501 accggagggg tatgacggct tcccgctcta cctggacgcg cagacgcccc tgccgtccaa
     1561 ggacgcttcc ctttggtaca cacgcaaacg cgacctttgg tggacactga accaagcctc
     1621 attgcgcctg gttcgcacgg gagaaaggac catccttgtc gagtgcggca acagccagcc
     1681 gttcttccgg cgctatgtcg cccgcatcgg agacgccgtc tggaagcccg tggacgcacg
     1741 cttcacctgg gagctcgtac cgggggataa ccggattgag gtcgtctcgg aagacgcctc
     1801 tggcagacga gcaatcatga gtagcgccgt ggtgaggctt gagcgctagt ttagaggtga
     1861 atcccccagc gtagatgcct gcgctcgagg acaacttctc ctttggccgc ttttcttacc
     1921 ttcagtccga ttgtctttct cgcacaaacc acaattaatc tactttccgt ggtagcggcc
     1981 acgactatac cagtgttata ctcaggctta cgccaccggc gttagattgg gcgactcttc
     2041 ctcgttgatt gatgggtatt catctgagcg ctcaagaaac ccgaactcat tggccgtgac
     2101 ttttgggctt tttttgttga acgacgacgc gttgagttga tgtcgtttcc gcgatcgttg
     2161 ggtttttgcg tcgcgccggt gtcagtactt gggacaaacc gcgcgtgccc ggctgcatgg
     2221 ccgggctggc tgaatcgaag gctcggcttc gatcatagcc agatgccgtg acactccgaa
     2281 tggacaactg ggagaatcct ttgaaactgg tattgagcct gctcggcaca atagcctttg
     2341 cgaccgcctg tctcgcggaa gacaacccgg gaaaggcgat cttcgatcgc acctgcaaga
     2401 catgccacgg accggatggc aagggagatg ctgcggcgga caagttctat cagatccgaa
     2461 ttcctcgtct gaactcagcc tatgttcagc agaagtccga cgaggaattg aaagagatca
     2521 tcacgaaagg ccggcgcaag atgaaaccgg tccagccggg ccagccgata gccgaacata
     2581 atctgaaatc ggagtggacg gatagcgtca tcagctacgt tcgcacgctg aaaaaaaaga
     2641 agtagaaccc gcggccggca accggttctg ttcctctgag ccccttgccg gccctaccgc
     2701 ggctctcagg tacctgcttt tcactcactc tcccggccgg tccaccacat cccctcgcaa
     2761 ttccagtcgc gcgccgagca gatccggctg gatctgcagc gcttcgtcga gtagtcggtg
     2821 cgcctccgcc tggtcgccct tgtgcttcag taccagcgcc agaagataac gcgaactcgc
     2881 gcgccgcata gccctaccgc tgtcgagctg gcttctggca aagccttcca tctccccgag
     2941 tatcctggac gctgcggctg tcttcccgag tctgttgagg gccagagcca tatgcacgtt
     3001 ctctgcgttc caactgtccc agtctcccga taggtgctca tatccggaag ccgctttttc
     3061 gtacgccgcc cgtgcttcgt cccgcctccc cagcgcctcg agggtccgtc cgatgtagta
     3121 gtgcgctcgc gggaccgatt ggaatccaaa atcatctacc ccgagcgaca caggcggtgc
     3181 aagggccgcc tcgaactgtc tcaaggcctc gtcgtacctg ccctcacgga aagccgctgc
     3241 gcccatgccg tatttgagaa accgccactt gtcccgtaat ccgtacgtcc ggtgccgcgg
     3301 accgaacttc cgcccggtcg ccagcttctc ggcctccgag tagcgcccct tggcgagatt
     3361 cgcgagcaac agcccctccg ccagatcgtc gtcctcgggc gaccgcgaca gagcccgctc
     3421 gagcagcgcc cgttgctcgt cgaaacgccc gctcttcgcg taaattgcgc tcaggtcgtt
     3481 gagagtccgg acatgcccgg ggtcgatggc gatcgccttt tcaagttgca ccgctgcctt
     3541 ctccacgcca cgtccctgct cctcgtacgc cagccccagc gcccgccgcg tgcgaaagtc
     3601 cgacgggctg atcccggctg ctgtctccca ctgtctgatc gcttcgtcgg tccttccctg
     3661 gtggtacaac aggcaagcca ggtagaaccg ggccaccgca tcgttcggat cccgttcagc
     3721 cgcctcccgc agcactgcct ctgtttcttc acgatagggg aaccggtcaa tgttcgccgc
     3781 ggccgcttgc gccttcttca gatactccgc cgaggcggcc tccgcccccg cccgcttcag
     3841 gcagtggccc agtgtgtagt aaaactccga tggtgtgccc cacgggtcgt ggttttcttg
     3901 ttccaccagc atgagtactt ccaccgcctc cttccaccgg ccgagtctgc gatagaactg
     3961 ggagacttcc agcccctcct ggctctgccc gcccatcagt tcacacagcg tcttgcgcgt
     4021 ctctgcaccg cgggccagga agaatcgctc cacggctgcc cattggttcg tcggatcgat
     4081 cgcttccacc tccgcgagac gcgcgagagc ccgctcacgt gcgccctgtt cccggtccat
     4141 cagcgccaga agcagcagcg cgtccaggta gtagccgttc gatctcaccg cgcgttccag
     4201 gtgttccgcg gctttgtccc gctcaccccg aagcagtgcc agcctcccaa ggtggtattc
     4261 gcggttggcg aaatacgcgc tatccggcgg gatgaaatac agactccgct ccccagcctt
     4321 ctcctgcccg agcgccagcc ggctcattgc caggtagtaa tgcgcttcga agaggtacgg
     4381 gtcccggtcg atcgccttct ccagatgctc cgccgccgtc cggtaacggc cactgttgaa
     4441 gtgatcgatc ccgaacatca ggtgcgcccg tgaatagccg gggtctcttt cgatcgcaag
     4501 gttgagcagt tccgcggcgc cggcagcgtt caactgcttg tatttcacct ccgctgccag
     4561 ggcgagttcc tcgatgctca tttcctgtgg ttctttcgct ggacgctcca gcggtcgcgt
     4621 gaatggagtg tactccttcc gtcccggact ttcgtccggc cgatgatagg tcagcacctc
     4681 cgagccctcg gcatccgcca ccgtcacgac catgccgcgc agcccgttct catcggacgc
     4741 aatcccgccg gcagcgtgcc ggaacggcgt ctccggcgcc aggtccgcga cggcgctcag
     4801 aatccgaccg ctctttgctg tcactgtgat ccgggcgccg ggcattgggc ggttcgccga
     4861 gagacccact gtcactgttg agttccggcc cgacgggtcc ggcttcacgt ccaggtgcag
     4921 cgccacgtcg cgagaggtgg ttgtcagtcc tccgatcccc gccaccggga accaccactc
     4981 gctccagctc tcgaccttgt gcggcgacag ccactcgtaa tagccgtccc aaacgtagcg
     5041 cccgctctgc acctcgatgt acggcccggc gttgtcggtg tacgcacgtt ccaggtacgt
     5101 agcacggtcg gagtatccga acgtccacat cttcatgccc tgtaccacgc gacggtcggc
     5161 gtaccggaac actccgtagt cgagatcctc gtgatacgcg ccttgaaaat cgtcgtagat
     5221 gtcaatgccg aatacgccca gcatgttctt gttgttccgg tcccagctat agtcgatacc
     5281 gttgaacacc ggccagtccc ccacctcgga ggtagtatgg ccgatggtcc gcgtcatcgg
     5341 gtacacgaaa cgcgtctgct cggtggcccg gaggcttccg ctgatccaga acatgtgcgg
     5401 ccggcgccca tcgcgggtgt tgtagcagaa gacgctcatc tgcaccacgg ccagcgccgg
     5461 gtagagccgt gccgagaagt tcaccttcat gcggtacacc ggatcggtcg tgccgagcac
     5521 cagcgagacg ctcccatccg cgtgccgaac caccctgtgc gaccagaaag gctcgccgta
     5581 gagcgtcagc atgtgcgtat cgaacgcgcc agtcagctcg attccgaata gcgggaaact
     5641 ccccttggag ttgtagttcg ccggtttgac gacatcgttc cggtagaaga cctcccgctt
     5701 gcggaccttg tcgtaaagcg agaagagccg ccctccgaac tcgggcaggt acgtcagctt
     5761 caggtattcg ttctctacga atagcgcccc gtacgtcctg ggcttcggct cgcccttcag
     5821 cggcaggata aacggcggta acgggtacat cccaccaacc gtggaaccgg cgaacaacgg
     5881 aggttgcgtt tctctcccca cgtgctcata cgccggtatg tcgattctgc cttcgctgac
     5941 cgtaacggtg gcgctccatc cgcacgaggc gcagagcgcc aggacggcgc acacagttct
     6001 catgccttct cccattactc aacgttgtac cgtgggagcc cctccggctg catctctcct
     6061 gtcataaggg aactgcgccc gtgagtgagc ggccctggtc acacgaaact tttttgagag
     6121 aagcgagggg gagccaacag gaaggctggg ggcctacgcg tcttcgctgg tgggatggcc
     6181 cgtccgccgc ggcaaccggt tcggaatgga gccccgccgt gggatgttgt cgcgtatgat
     6241 ccacccggcc atcggcggaa ctacgggatg ctgtcggaga tcgtcttcca gttcttgaag
     6301 ctcccgatcg acaattgctc tttcgacccc ccagccgatc atggtcgatc cttcgagcgc
     6361 ccgtcggaga agaatccgct ggaggatgtg gaaaccgcgt tccaaacgcg ctttcgaact
     6421 ctctgctgtc ttcatgttgc ggcctccttc ctccattcac tcagatcatg ccccaacgtc
     6481 ggtctacgga aaatagcaaa agcggttcat ttcgatgttc cttagtactg gaactaccgc
     6541 ttgccccact gtcagcggta ccatgcaaga ccctccacca cgacgagcac atcagccctc
     6601 agcaagccga gggccgaaac ctacgactcc gtgctccccc gtgtaaacct gccgaaggcg
     6661 aacccgtcaa accagggtga gaggtgccaa aggagaaatg aagattctca cagtcttggt
     6721 atttgtttcc acgagcgtgc tgtttgcgca aggtactccg gctgaccggc ccatgggtcc
     6781 tcggcccctg cggggtccgg tcgccacgga tgaagttaag gcgtacctga acctcgagca
     6841 agcccaagtc gaccagcttg tccagcttcg caaggacgag agggacgaaa tgaaggctgt
     6901 cttcgagaag attaccgaga accggaaggc cctgcgggag gcgcttcagt caaccagtcc
     6961 cgatgcctcc acgctcggta acctgttgat cgatatgcaa aagctgcgga aacagatccg
     7021 cgaaacgaat gaaaactacc atacgaaggc gctcgccgtg ctgaaggagg agcagaagtc
     7081 gaaactcgcc gaactggaag cggcagcgaa gctccgtccg gtcatcggcc aggcgcacgg
     7141 cctcaacctg ctactgccgc ctgcgcagga agcgcgggat gaagtcatgg tcggcggtga
     7201 aggcgccggc ttcggaccca tggggatggg gatgcgtatg cgtaggttcg gagccgtggg
     7261 ccggccacgc cgttgaggcc cccttcgtgt aagcctgccc agcctgccgg tcgagcgccg
     7321 gcaggcttaa ttcctgtaaa ttcggaagaa gtgccaagaa aggcggaagc cccgggtctg
     7381 ggggcccggg gcttcactca gaggggctag cgacgagctg ggactcgtcg ccggggatgc
     7441 tataagtcaa tgacgactta catttgctac tatctgaagc attttgcctg ccaaagaccg
     7501 catgctgcat ctaatagact tacccatctg aaactgactt ggagtgcctg tggccggtgg
     7561 gtcttttcgc aagggtgagg acattcccgc acccaagccc agtcctcatc cgcggcgagg
     7621 ctcgccagca tgcgccagcg ctggctcgat cacttgctcg atctccgcat ggatctctgc
     7681 cggcgtacgg tcaccctcga tccggtgaaa atcgcgggtt gcatagaagt ccaccagcgg
     7741 cccggtcgcc tcgtggtacg ctttcagccg cgccagaatc gtctgctcat tgtcgtcctc
     7801 acgctggatc agaggagtcc cgtcgttatc gcacacaccc gcatggcgag gcggctggtg
     7861 gagcaggttg tagatcgcgc tgcatgtagg acactgccgg cgcgacgtga tccgagctac
     7921 cagaaccgaa tccggaacgt cgaggtagat caccgtcgga gcgggaagcc tctgctcctc
     7981 cagtaatcgg tccagaaact cggcctgcgc cttggttctc gggtagccgt cgaggatgaa
     8041 cccgtttcgg cagtcatcct gactcaatcg gccggcaacc atctggttga cgatctcgtc
     8101 gctcaccaga ccgccactgc gcagaatctc gcaggcaagc ttgccaatct tcgtgccggc
     8161 cttgcattcc gcgcgaaaaa tctcaccggt cgaaatcgac ggaacaccca tcgaccgtgc
     8221 catggagacc gcttgggttc ccttgccgca tccaggcgga ccgaatagca gaatgaccat
     8281 gttctctcct tgggcaacga tcgtaccggc tcaaaacgca cagaaaagaa taaataaggc
     8341 cctttgctcg gattcgatgc cctagtttga gactatccta tccgaaactg aacagtcaag
     8401 gcagccgcgc gcccacgcac tccctttcat tcgcgggcat tccgttctgt gggggatttc
     8461 acgcagaatg ctgcccgctc ggggcggtgt gcctcagatt cgtctcaacg gcattagtct
     8521 tgaggctgga aagagcccta ctgtctagca cttgcgagtg gtgctcgtaa ctccgcaatt
     8581 ggaatcacaa ttgcctcttc ccattgcaga ggatactatg gcttacgtta tcgttgatac
     8641 ctgcacaaag gacaaactct gcattgaggc ctgcccggtg gactgcattc atccgaagga
     8701 agacgagcca aaattcgagg gagtgccgca actcttcatt caacccgaag agtgcatcga
     8761 ttgtggcgcc tgcgtgccgg tgtgtccgac gaactcaatc tacgttctcg acgagctccc
     8821 cgccaacctg gcgcagttcg cggagaaaaa tgcggcctac tacgcctgag attgggtgct
     8881 cgccgtttct ttcttaagcc ttatttttca gtgagttgca gcttccgtgg cgcatctgtt
     8941 cccaagatgc gtcacagttg cttctttgct ttgtgcggaa gggtgatgta gctggcgcca
     9001 aagtagctgt aaacacagcg gcccgaatcg atcaactggt ggatggctcg tcggcaattg
     9061 tccttcgagc acctctctcc gaacctctcc atcatcattt gcgtgagatc ccccgccttc
     9121 aagtagcgct taccctgctg ctggcccacc acgcggtaga tttcatgggc aagggtttcg
     9181 atagagacct cattattcat ggtcttaaga cctttttatc cttaattacc atggccgtgt
     9241 ttgcaagccg tgggccaaag cgccgggtag gagtggctcc acggccgaat tagcggtaag
     9301 ctgcaagtga gagggatcaa cggcgtgaag cacgttgtag gcgcggcgat gttcgtagcg
     9361 gccagtgcat gcatgttcgc ccagcagcga ggcgct
//
LOCUS       DATIFQ010000031         3220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2690776, whole genome shotgun sequence.
ACCESSION   DATIFQ010000031 DATIFQ010000000
VERSION     DATIFQ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2690776"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VLE22_01250"
     CDS             complement(<1..632)
                     /locus_tag="VLE22_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HSB13051.1"
                     /translation="MIRRQFLQGSMGLGAMAQAPGSAPHSPAAYNVRSFGAAGDGEHL
                     NTAGIQAAIDACAKGGGGAVLFPAGRYLSGTIFLKSRVTLYLDAGAVLLGSRKLEHYP
                     VTVPAFRSYTDNYTDKSLIYAENVEDIAIEGRGVIDGQGAAFQGPYKVRPYMIRIIQC
                     RNVSVTGVTMKDSPMWVQHYLACDGVTIRGLTVHSRVNHNNDGIDIDCCER"
     gene            complement(645..1889)
                     /locus_tag="VLE22_01255"
     CDS             complement(645..1889)
                     /locus_tag="VLE22_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB13052.1"
                     /translation="MPRLRWWIAALLFLATVINYVDRQTLSVLAPVLRDEFRMSNTDY
                     SRILFAFLFAYMIMQAGSGRIMDRLGTRLGFALTISWWSVAAMLHAAANSAFALGACR
                     LLLGLGEAGNWPGAVKAISEWFPPKERAFAIGFFNSGSTIGAVIAPPLVTWIALTWGW
                     RRAFLLTGALGFLWLIAWLLLYRKPSEHPGVDRAELDALQEAQAQGAEAGGAPVRWRD
                     LLRVRQVWGLVLGRMLADPVWWFYVFWLPEYLRREREFSLAMIGYFAWIPFLTADLGC
                     LAGGWASGFLVRRNWAVLRARKAIMLVCAALLVSGIPAVLAKDAMISLALISLATFGY
                     SAWASNILTLPADLFPPRIVASVSGLSGTGAALGGMIFTLIVGAVVDRFSYVPVFVAA
                     GLMPLVSVSIILWWVRPVASGE"
     gene            1951..3120
                     /locus_tag="VLE22_01260"
     CDS             1951..3120
                     /locus_tag="VLE22_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB13053.1"
                     /translation="MTGRERILAAFHGGKADIVPFCPNIYYWFYNRLVTGTLPEEIAS
                     ARHPFDALRVLGADILARWDTQGATREVWSAGEYSDDFGGSRGQDQPAVTAFNVYPPG
                     RSIRRQRFATPHGVLSQTWVYTAEAGADFLCEYWWKDWSEYEAVRYMLEARDYRFDRE
                     EFRHWVARVGDDGVMMVHLTQSPLKTFHWLAGPENASLFSIDHPDEMKELVRIQEAKA
                     LALLETVVDLPEAEIFISLDNLDSAFFPPYFYRDYCESFFRRAADIVHSRGKIFVVHA
                     CGHNRLLLPHVGNSRVDCLEGLTPPPMGDVELCEARTLAGWPNYTVNGGMDASRLDMA
                     TDAEAGIHAYTRDLFASMGDKRRFIFASSCSTPARTPWSNLKHFRDAAREYGAVG"
BASE COUNT          598 a         1060 c         1039 g          523 t
ORIGIN      
        1 cgctcgcagc aatcgatgtc gatcccgtcg ttattgtgat tcacgcggct gtggacggtg
       61 aggccgcgga tggtcacgcc gtcgcaggcg agatagtgct gcacccacat cggcgagtcc
      121 ttcatcgtca cgccggtgac ggacacgttc cggcactgga tgatccggat catgtacggc
      181 cggactttgt atggaccctg gaatgcggcg ccctgcccgt cgatgacgcc gcggccctcg
      241 atggcgatgt cttcgacgtt ctctgcgtaa atgaggctct tgtcggtgta attgtcggtg
      301 taggaccgga acgcgggcac ggttaccgga tagtgctcca gcttgcggct gccgagcagc
      361 acggcgccgg cgtcgaggta caaggtgacg cggctcttga ggaaaatcgt tccgctgaga
      421 taccggcccg ccgggaacag caccgcgcct cctccacctt tcgcgcaggc gtcgatcgcc
      481 gcctggatgc cggccgtgtt caggtgctct ccgtctcccg ccgctccgaa cgagcgtacg
      541 ttgtacgctg ccggagaatg tggcgcggaa ccgggcgcct gtgccatcgc cccgagaccc
      601 atcgatccct gcagaaattg acgccggatc atcactcgcc tccttcactc gcccgaagct
      661 accgggcgga cccaccacag gataatggaa accgacacga gcggcatcag cccggcggca
      721 acgaacacgg gaacgtacga gaagcggtcg accaccgctc ccacgatcag cgtgaagatc
      781 atgccgccca acgcggcccc ggtgccgctg agtccggaca ccgacgccac gatgcgcggc
      841 gggaacaggt ccgccggaag cgtcaggatg ttcgacgccc atgcggagta gccgaacgtg
      901 gccaggctga tcagcgccag gctgatcatc gcgtctttcg ccagcaccgc cgggattccc
      961 gaaaccagca gcgccgcaca caccagcatg atggccttgc gcgcccgcag cactgcccag
     1021 ttccgcctga cgagaaagcc ggaagcccac ccgcccgcga gacacccgag gtccgccgtc
     1081 aggaacggga tccacgcgaa gtagccgatc atcgccaggc tgaactcccg ctcgcgccgc
     1141 aggtattcgg gcagccagaa cacgtagaac caccacaccg gatctgcgag catcctcccc
     1201 agcaccagcc cccaaacctg cctgactcgc agcagatcgc gccatcgcac gggcgcgccg
     1261 ccagcttcgg cgccctgtgc ctgcgcctcc tgaagcgcat cgagttccgc gcggtcgact
     1321 ccggggtgct cggacggctt tcggtaaaga aggagccacg cgatcagcca gaggaaacca
     1381 agcgctccgg tgagcagaaa cgcccggcgc cagccccacg tgagcgcgat ccaggtgacc
     1441 agcggcggag cgatcaccgc gccgatggtg gaaccgctgt tgaagaaacc gatcgcgaag
     1501 gcgcgctctt ttggcggaaa ccattccgag atggccttca ccgctccggg ccagttgccg
     1561 gcttcgccga gccccaacag cagccggcag gcgccgagag cgaaggcgga attcgccgct
     1621 gcgtgcagca tggccgcaac cgaccaccac gagatcgtca gcgcgaatcc gagccgcgtg
     1681 cccagccggt ccatgatccg cccggagccg gcctgcatga tcatgtaggc aaacaggaag
     1741 gcgaacagga tgcgggagta gtccgtgttg ctcatccgga actcgtcgcg cagaaccggt
     1801 gcgagcacgg acagcgtctg gcggtccacg taattgatga cggtggcgag aaatagaaga
     1861 gcggcgatcc accagcggag gcgcggcatt caggcggccc gctccacgag tggatccggc
     1921 ggccggaccg ccgcgtgtac agtagagtgg atgaccggga gagagcgcat tctggccgcc
     1981 ttccacgggg ggaaggccga cattgtaccg ttttgcccca acatttacta ctggttctac
     2041 aaccgcttgg tgaccggcac gctgcccgag gagatcgcct ccgcccggca tccgttcgac
     2101 gcactgcgcg ttctgggagc cgacatcctg gcgcgatggg acacgcaagg ggcgacgcgc
     2161 gaggtttggt ccgccggcga gtactcggac gatttcggcg ggtcccgcgg ccaggaccag
     2221 cccgcggtaa cggcgttcaa cgtgtacccg ccgggccggt ccatccgccg gcagcggttc
     2281 gccactccgc acggagtcct cagccagact tgggtgtata ccgcagaggc gggcgcggat
     2341 ttcctgtgcg agtactggtg gaaggactgg agcgagtacg aggccgtccg gtacatgctg
     2401 gaagcgcgcg actaccggtt tgaccgcgag gagtttcgcc actgggtggc gcgcgtcggc
     2461 gacgacggcg tgatgatggt gcacctgacg cagtcgccgc tcaagacgtt ccactggctg
     2521 gcgggtccgg agaacgccag cctgttctcg atcgatcacc cggacgagat gaaggaactg
     2581 gtgcgcatcc aggaagccaa ggcgctggcc ctgctcgaaa cagtggtgga cctccccgag
     2641 gctgagatct tcatctctct cgacaatctg gactcggcat ttttcccgcc atatttctac
     2701 cgggactact gcgagagctt cttccgtcgt gcagcggaca tcgtccacag ccgcggcaag
     2761 attttcgtgg tgcatgcctg cggtcacaac cggttgcttc tgccgcacgt cggcaactcc
     2821 cgtgtggatt gcctggaggg actcacgccg ccgccgatgg gagacgtgga gctttgcgag
     2881 gcgcgaacgc tggcgggctg gccgaattac acggtgaacg gcggtatgga cgcctcgcgc
     2941 ctggacatgg ccactgacgc cgaagccggc atccacgcgt acacgcgcga cctgttcgcc
     3001 tccatgggcg acaaacgccg cttcatcttc gcgtcaagct gctcgacccc cgcccgcaca
     3061 ccctggagca acctgaagca ctttcgcgac gctgcgcggg agtacggggc tgttggatag
     3121 gattcggtcg agtcgcgcga atgctcgggc tgtctttgaa gctaagtccg agcgcgatga
     3181 tcggagcgtt acagatttgc ggttttggcg taatacggcc
//
LOCUS       DATIFQ010000032         6558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_9258, whole genome shotgun sequence.
ACCESSION   DATIFQ010000032 DATIFQ010000000
VERSION     DATIFQ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9258"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VLE22_01265"
     CDS             complement(<1..572)
                     /locus_tag="VLE22_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13054.1"
                     /translation="MTRAVSLLLVCVALVPGQSGIGPPLAGLVRDPCGNLRPVLGVAG
                     SFITGDILSTQVESVSFSGDLGFVKTAKSIQVFDAEGGLISEQAAPPGPALFAIPPDG
                     RGAVAFLPVTGEWWRWSRGGFSPFEVEPDLAGAEVLALTMPVGDRLSLVIRTGGGTSL
                     AALSLRTGRVHAGAPLDGASVPALLRADGTL"
     gene            complement(569..1165)
                     /locus_tag="VLE22_01270"
     CDS             complement(569..1165)
                     /locus_tag="VLE22_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13055.1"
                     /translation="MVRRLCLFLPLLLASSVKPAVLQIHVVHGDRAVHQPGSRVQTPL
                     VVKITGETGEAVDGALVSFRLPASGPGGLFANGLATDIVRTGADGAAAMPPVRWNTSP
                     GSFEIRVTAAKDAARAGLMVSQSLGEAQTTSMVTVHPGAASARPRRKWILLVAGVAAG
                     SLAAGMAVAKGRTDSSSAAPATPGLSVGTPSISIGGPK"
     gene            complement(1211..1927)
                     /locus_tag="VLE22_01275"
     CDS             complement(1211..1927)
                     /locus_tag="VLE22_01275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HSB13056.1"
                     /translation="MLEPFGLTHLGSVRKNNEDSYLIVPELGLYLIADGMGGARAGEH
                     ASRIAVETVAECAKASGRRSPRLLLEAFEEAHERVRAAATADPNLEGMGTTLVAALEV
                     GDELLIASVGDSRAYLFRDHQLTMITTDQTWVAEVGRPLGISEEDLKNHPLRHVLTMA
                     LGANAPLKAQSYAVPITPGEVVLLCTDGLHGAVDDETIRGALDSEQSLESCAHYLIDA
                     AIQAGGPDNVTLVLLRAPGQ"
     gene            2434..3996
                     /locus_tag="VLE22_01280"
     CDS             2434..3996
                     /locus_tag="VLE22_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HSB13057.1"
                     /translation="MRHVLREIRQTGISRLTQVVDSISGTITLTWDDFDRLTREVTPQ
                     GTVDYVYDAAGRRVTMTVAGQPIVNYSYDNADRLTGITQGSSTVTFEYDNADRRTKTT
                     LPNGVSMEYFYDHAGQLTRIDYKQGTTLIGDLTYEYDAAGNRIAMGGSFGRTGLPQPL
                     ASAVYDAANRLTQWGGTTLSYDDNGNLTSDATNSYTWSARNQLASMTGASFQYDAFGR
                     RKSKTIGGASTQFLYDGVNVVQELSGTTANLLTGLGVDEMFTRTDSAGAGHYLTDGLG
                     STLALTDPTGAVQTRYTYEPYGKVSVSGTSSANPFQYTGRENDGTGLYYYRARYYHPV
                     LQRFISEDPIEFEAGDPNFYGYVFNDPIGAYDPDGLSIAGKIFKQTVKGAWRLIRNKT
                     AQQQLAKKCDLNYATPSGGRRGLRAMKKLSPGETVKGHTKDVTAREYGPHVQTSPNPK
                     RNHLFFGRELYSITGPLTLSYYAEGRGWWTETASALADLFNPLSIPNDIVEVADELNG
                     IAHEVLPFSGRK"
     gene            4006..4509
                     /locus_tag="VLE22_01285"
     CDS             4006..4509
                     /locus_tag="VLE22_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13058.1"
                     /translation="MLLFRERYPGLDLNTVRYVDEPAPVYSSDSFDLQFRKRLGEELG
                     LLEPVRCWTIAFFRIVRSINCVCCRLGLPVVRTMTLEQCVEHVQDHLTKLHREVQSDE
                     PGGWKSEFQICSFIEPKVGLSIIFRFKSAHNMETSWLVLGEKVILKRRGGKYNVFELR
                     EEEDNNA"
     gene            5009..5248
                     /locus_tag="VLE22_01290"
     CDS             5009..5248
                     /locus_tag="VLE22_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13059.1"
                     /translation="MQTRINVIQVLLLFLALFLAMYLGQYSAKYIGWLAWAPAVVLGL
                     ILAIVGVFCAVMNVLETHRWRDERTKQEKSGEHRE"
     gene            5384..>6558
                     /locus_tag="VLE22_01295"
     CDS             5384..>6558
                     /locus_tag="VLE22_01295"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13060.1"
                     /translation="MYLTNQYDANGRVSRQTLVDTGVWLYSYTLDAQGKVKSSEVTDP
                     RGLVIKKAFNTDGYVLTDTFISGHDQLKLTFDRQAGTNLITRVTDALSRKTDYGHDAC
                     GNVTSVTRMAGTSQAATTSYTYQTASDCLATYNRLTGVTDPLNHTTGIGYDAAGNLTS
                     IQGPIPSIPATTFGYNTAGQVTSVTNPLSKTWQFGYEYGDLNTVTDPLQRTTTRVTDY
                     AGRLGMMENALGQRTHLEYDVLNRLRKITDPLTGKTEFTYDPNGNLLSVKDARSGTTG
                     YAYDDNGNLTGDGVNTYTWDARDRLVSMTGANFQYDPFGRRIGKTTGGTTTQFLYDGV
                     NPLQEIAGTTTGLLTGLGIDQVLTRTDSSGTRHLLTDALGSTVALVDPSGLVQTQYT"
BASE COUNT         1378 a         1930 c         2081 g         1169 t
ORIGIN      
        1 agcgtcccat ccgcgcgtaa cagggcgggg acagaggcgc cgtcgagtgg agcaccggcg
       61 tgcacccggc ccgtccgcag cgagagcgcg gccagggacg ttccgccgcc ggtccggatg
      121 acgagggaga gacggtcgcc caccggcatc gtgagggcga gcacctcggc gcctgcgaga
      181 tcgggttcca cttcgaaggg actgaatccg ccgcggctcc agcgccacca ttcacccgtt
      241 accggcagga acgcaaccgc gccgcggcca tccggcggaa tcgcgaacaa cgcgggtccg
      301 ggcggtgcgg cctgctccga aatgagtccg ccctccgcgt cgaacacctg aatcgatttc
      361 gcggtcttca cgaaaccgag gtctccggag aaggacacgg actcaacctg tgtcgagagt
      421 atgtcgccgg tgatgaaact accggccacg ccaagaaccg ggcgcagatt gccgcaaggg
      481 tcgcgcacaa ggcctgccag cggaggtccg atgccggact ggcccggaac gagcgcgaca
      541 catacgagaa ggagggacac cgcccgggtc atttcggccc tccgatgctg atggacggag
      601 tacccacgct caggcccgga gtggccggcg cggcggagga ggaatccgtg cgccctttcg
      661 ccaccgccat tccggcggcc agcgagcccg cggcgacgcc ggcgaccagc agaatccatt
      721 tccgccgcgg tcttgccgag gccgcgccag gatgcacggt gaccatggaa gttgtttgcg
      781 cttcgccgag ggactgcgag accatcagtc ccgcgcgggc agcatccttc gccgcggtca
      841 ccctgatttc aaaggagccg ggagaggtgt tccagcgaac gggcggcatc gcggccgctc
      901 cgtcggcgcc ggtgcgcacg atgtcggtgg ccagtccgtt tgcaaacagg ccacccggac
      961 cgctcgccgg cagccggaag ctgacgagag cgccgtcgac ggcctcgccg gtttcgccgg
     1021 tgattttcac gactaacgga gtctgtaccc gggagcccgg ctggtgaacc gcgcgatctc
     1081 cgtggacgac gtggatttgc agcacggccg gtttgacaga ggatgccagc aggagcggca
     1141 ggaagagaca caatcgcctg accatgtttc cctcggcgcc gcaacggaaa caggccggtt
     1201 ccggaggaat ctattgcccg ggcgcccgca acaacaccaa ggtgacgttg tccggaccgc
     1261 ctgcctgaat tgcagcgtcg atcaggtagt gggcgcagga ctccaaagat tgctccgaat
     1321 ctaacgctcc acggatggtt tcgtcgtcca cggctccgtg aaggccgtcc gtgcagagca
     1381 ggacgacctc ccctggcgtg atcgggaccg cgtaagactg ggccttgagg ggggcattcg
     1441 cgccgagcgc catggtcagg acgtggcgca gaggatggtt tttgaggtcc tcctcgctga
     1501 tgccgagggg gcggccgact tcggcgaccc aggtctgatc ggtggtgatc atcgtgagct
     1561 ggtggtcgcg gaagaggtag gcgcggctgt cgccgacgct ggcgatgagc agctcgtcgc
     1621 cgacctcgag cgccgccacc agagtggtgc ccatgccttc gaggttcggg tcagcggttg
     1681 ccgccgctct cacacgctcg tgggcttctt cgaatgcttc cagcaggagc cgcggactcc
     1741 gccgccccga ggccttcgcg cattcggcaa ccgtttcgac ggcgatccgg gacgcatgct
     1801 cgccggcacg cgccccgccc attccatcgg caatcagata gagcccgagc tccgggacga
     1861 tcaggtagct atcttcgttg ttctttcgaa cgctgccaag atgggtgagg ccaaagggtt
     1921 ccagcatcgg ctatccgaat tatatccgca gggacgggcg ggtggtaagg ccgatctggc
     1981 ccgctccctc ggcgtgaaat gcgaaagtat ccggtggatc tgatcgattg agttctcggg
     2041 ggcagggcga gagtgcacgc ggacgttaac ggaagagatt gagattcaaa ggatgcgcga
     2101 cgcgaccatg gggcatgaca gggggcgcgt ccaggctaac gcaaggtcag gggagcggcc
     2161 gtttgcggcc gcgcaagcga gcgtgaacgc caggaccgag agggagcggc ccgttggtca
     2221 ctgggctact caaacaccgc cgttagcgga atagcagcgc ctgctgatgc gcgcggctct
     2281 cttaacgtaa ccataagtcc cgagttacga ccgctcaaaa gtaccaaagt atctttgaga
     2341 agatttcgta agattttcga gttagaaata tatataggag tttcataccc attaaaccta
     2401 ttgacatccg cgattgcccc gaatattctt gggatgcgcc atgtgctgcg tgagattcgt
     2461 cagacgggga tcagccggct gacgcaggtg gtagattcga tctcagggac gatcactttg
     2521 acgtgggacg atttcgaccg gctgacgcgg gaggtgacgc cgcaggggac ggtcgattac
     2581 gtctacgacg cggcggggcg gcgggtgacg atgacggtgg cggggcagcc catcgtgaac
     2641 tactcgtacg acaacgcgga ccggctgacc gggattacgc agggaagctc gacggtgacg
     2701 ttcgagtacg acaacgccga ccggcgcacg aagacgacac tgccgaacgg ggtcagtatg
     2761 gagtacttct atgatcacgc cgggcaactc acgcgcatcg actacaagca agggactacg
     2821 ctgatcggcg acctgacgta tgagtacgac gcggctggca accggatcgc gatgggtggc
     2881 agcttcgggc gaacggggct gccgcagccg ttggcctcgg ctgtgtatga tgcggcgaac
     2941 cggctgaccc agtggggcgg aaccacgctt agttacgacg acaacggcaa tctcaccagc
     3001 gacgcgacga acagctacac gtggagcgcc cggaaccagt tggcgtcgat gacgggggcg
     3061 agtttccagt acgatgcgtt cgggcggcgg aagagcaaga cgatcggcgg tgcgagcacg
     3121 cagttcctgt atgacggagt gaacgttgtc caggaacttt cggggacgac ggccaacctg
     3181 ctgacggggc tgggcgtcga tgaaatgttc acccgcacgg actcggctgg cgccgggcat
     3241 tacctgacgg atgggttggg ctcgactttg gcgctgacgg acccgaccgg tgccgtgcag
     3301 acccggtaca cttatgaacc ctacggcaag gtgagcgtga gcgggacatc cagtgccaac
     3361 ccgttccagt acacgggccg cgagaatgac ggcacgggac tgtactatta ccgggcgcgg
     3421 tactatcatc ccgtgttgca acggttcatc agcgaggatc caatagaatt cgaggctgga
     3481 gacccgaact tttatggtta tgtcttcaac gacccgatag gcgcatacga tccggatgga
     3541 ttgtcgattg ccgggaagat cttcaaacaa acagtcaagg gtgcttggcg cctcattcgt
     3601 aacaagaccg ctcagcagca actggccaag aaatgtgacc tgaactacgc cacgcccagt
     3661 ggtgggagga gaggactgag ggcgatgaag aagctcagcc ctggagaaac cgtgaagggg
     3721 cacacgaaag atgtcacggc tcgcgaatac ggtcctcacg ttcagacctc accgaatccc
     3781 aagcgaaatc atctgttctt cggacgagaa ctctattcga taacaggtcc actgactttg
     3841 tcctattatg ccgaaggtcg aggatggtgg acggagacgg cttcagcgtt ggctgacctg
     3901 ttcaatcccc ttagcatccc aaacgatata gtcgaagtcg cggacgaact gaatggaatt
     3961 gcacatgagg ttctcccgtt ctctgggagg aagtaagagt tccatatgtt gctctttcgg
     4021 gagcgttacc cagggctgga tctcaacacg gttcgatacg ttgatgagcc tgctccggtc
     4081 tacagctcag attcgtttga tctgcaattt cggaaaagac tcggcgagga gcttggcctt
     4141 ctggaaccag tacgatgctg gacgattgct tttttccgga ttgtcagatc cataaactgc
     4201 gtctgttgcc gtttgggact ccccgtcgtg cgcacaatga cccttgagca gtgcgtggag
     4261 cacgttcaag accacctgac caaactgcac cgtgaagtcc agagcgatga accgggcggc
     4321 tggaagtctg aattccagat ctgttctttc atagaaccaa aagtcggact gagtatcatc
     4381 ttccgcttca agtctgcaca caatatggag acaagttggc tcgtgctcgg tgagaaagtc
     4441 atcttgaagc ggcgtggcgg aaagtacaac gtattcgaat tgagagaaga ggaggataac
     4501 aacgcatagt gattcgccgt ctcttgaggg tgcttcgcgc tgccgtcaaa cctcctcagg
     4561 gcgaggtgaa gggataccgc cccggaatga cacctgcggc aacgtggctt ccatcatgcg
     4621 gctggcgggg acacaggagg cgttgaccac gacgctgaca tatcagccgc cgggagcgac
     4681 ctgcgcttcc acgttcaact aggtgaccag cattaccgaa ccgatgggcc acacgacaac
     4741 cttcggctac gaccacaacg gcaaaccgac aagcgacggg gtcaatgtgt acggtgacgc
     4801 cgaccaactg attcgaatcg actacaagca agcgaacact gctcggcgac ttgacgtatc
     4861 agtacgaccc cgccggcaaa cgggtcgcca tgggcggcgc ctttgcccgg acaggcttgc
     4921 cacagccttt aacgagcgct gtctacgaca cggcgaaccg gctcacgcag tggggcgcaa
     4981 cgacgctgac ctacgacgag ttgtggttgt gcaaactagg atcaacgtca tccaggtttt
     5041 gttgttattc ttggcgctgt ttctggccat gtacttgggg caatattctg cgaagtacat
     5101 cggttggctg gcttgggctc ccgctgttgt tctcggtctc attctcgcca tcgtgggggt
     5161 cttctgtgca gtgatgaacg ttctagaaac tcaccgttgg cgcgacgaga gaacgaagca
     5221 agagaagtcc ggggagcatc gtgagtaaga agcctgtgtg ctcgccttcc tgcttgatta
     5281 ctcgtgttcg taaaagcatt acaaagcatg ccctgtgcct gactattatg acccgtcaga
     5341 agaattcatg ggtgaacccg agctggggta ggttgccggc atcgtgtacc taaccaacca
     5401 gtatgacgcc aacggtcgag tcagccggca gacgctggtg gacaccggcg tctggcttta
     5461 cagctacacg ctcgacgccc agggcaaggt gaaatcctcc gaggtgaccg atccgcgggg
     5521 gttggtgatc aagaaggcct tcaacaccga cggttatgtg ctgacggata ccttcatctc
     5581 gggccacgac cagctcaagc tgaccttcga ccgccaggcg ggcacgaacc tgatcacgcg
     5641 ggtgaccgat gcgctgagcc ggaagaccga ctacggccac gacgcgtgcg gcaatgtcac
     5701 ctcggtgacc cgcatggccg gcacctcgca ggcggcgacc acgagctaca cgtatcaaac
     5761 cgcctcggac tgcctggcga cgtacaaccg gctgacgggc gtcaccgacc cgctgaacca
     5821 caccaccggc atcggctatg acgcagccgg caatctgacg agcatccagg ggccgatccc
     5881 gagcatcccg gccacgacgt tcgggtacaa caccgccggc caggtgacct cggtgacgaa
     5941 cccgctgagc aagacctggc agttcggcta cgagtacggc gacctgaaca cggtgaccga
     6001 cccgctgcaa cgaacgacca cgcgggtgac ggattacgcc ggccggctgg ggatgatgga
     6061 gaatgcgctg gggcagcgga cgcatcttga gtacgatgtg ttgaaccgtc tgcggaagat
     6121 caccgatccg ctgacgggca agacggaatt cacttacgat cccaacggca acctgctcag
     6181 cgtgaaagac gccaggagcg ggaccacggg ttacgcttac gacgacaacg ggaacctgac
     6241 cggcgacggc gtgaacactt acacgtggga cgcgcgcgac cggctggtgt cgatgaccgg
     6301 ggccaacttt cagtacgacc cgttcgggcg ccggatcggc aagaccaccg gcggcaccac
     6361 cacgcagttt ctctacgacg gcgtcaatcc tctccaggag atcgctggga ctacgaccgg
     6421 cctgctgacc ggcctcggca ttgaccaggt tctcacgcgc acggattctt ccggcactcg
     6481 tcaccttctg acggacgccc tgggctcgac cgtggcgctc gttgatccaa gcggcctcgt
     6541 acagactcaa tacaccta
//
LOCUS       DATIFQ010000033        15736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2691627, whole genome shotgun sequence.
ACCESSION   DATIFQ010000033 DATIFQ010000000
VERSION     DATIFQ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15736
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691627"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VLE22_01300"
     CDS             complement(<1..346)
                     /locus_tag="VLE22_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13061.1"
                     /translation="MNPLAEDLDHVLVHTQGLWDELRGQRIFITGGTGFFGCWLLESF
                     TWANDKLGLGASATVLTRNPEAFRKKAPHLAGHPSVRLHSGEIASFSFPEGEYAYVIH
                     AATENDFALDDSR"
     gene            complement(343..1674)
                     /gene="rfbH"
                     /locus_tag="VLE22_01305"
     CDS             complement(343..1674)
                     /gene="rfbH"
                     /locus_tag="VLE22_01305"
                     /inference="COORDINATES: protein motif:HMM:NF011936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HSB13062.1"
                     /translation="MSERARQLRDRILGLVAEYHEAAHAGGEFIPGVSPVPVSGRVFD
                     AGDMQSLVDSALDFWLTAGRFAAEFEKQFARYCGVRDALIVNSGSSANLVALSALTSP
                     KLGDRRLTPGDEVITIAAGFPTTVNPIVQNRLVPVFVDITVPTYNIDVSRLDDALSEK
                     TRAVMIAHTLGNPFDVSAVTAFARRHGLWFLEDCCDALGAEYEGRRVGSFGDLATFSF
                     YPAHHITMGEGGCVVTDQPLLQTLAASFRDWGRDCWCEPGKANTCGKRFDWQFGDLPH
                     GYDHKYVFSHVGYNLKATDMQAAVGLAQLRKLAGFIEARRRNFRALCEGLRDLEEFFV
                     LPRATPGSNPSWFGFPVAVRPEAPFSRADVVRYLEERKIATRLLFGGNLLRQPAYRDI
                     PHRVAGDLRNSDFVMNHLFWVGVYPGISAEMVNYMLETFHRVPVAMENALR"
     gene            complement(1671..2654)
                     /locus_tag="VLE22_01310"
     CDS             complement(1671..2654)
                     /locus_tag="VLE22_01310"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSB13063.1"
                     /translation="MVLNGTFWRDRPVFVTGATGLLGGWLVRRLLEQGADIVCLVRDW
                     VPQSELVCARLIDRVKIVRGDLRDREVLERTLAEYEIDSVFHLAAQTIVGIANGNPIS
                     TFESNIQGTWNVLEACRRSPKVHAIIVASSDKAYGEQKQLPYTEDCPLQGRHPYDASK
                     SCADLIAQTYAATYGLPVAITRCGNFYGGGDLNWNRIVPGAIRSVLRGERPVIRSDGQ
                     FVRDYFYVEDGAAAYMLLAEKLRGSPEFAGSAFNFSNEAQVTVLELVERILTRMGSAL
                     VPDVRNEASNEIRRQYLCAERARRLLNWAPLFTLDQGLERTIHWYREFLSS"
     gene            complement(2645..3421)
                     /gene="rfbF"
                     /locus_tag="VLE22_01315"
     CDS             complement(2645..3421)
                     /gene="rfbF"
                     /locus_tag="VLE22_01315"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HSB13064.1"
                     /translation="MKVVMLCGGLGTRLREETEFRPKPMVEIGGRPILWHIMKMYAHH
                     GFRDFVLCLGYRGNMIKEYFLNYEAMNNDFTICLGQRSQIHYNGVHTEQDFRVTLADT
                     GLDSMTGGRIRRVQKYLDGGTFLVTYGDGLADLDIGRLIDFHKSHGRLATVTTVRPVS
                     RFGSVALDEEGSVKQFSEKPRSDGWISAGFFVFEPGIFDYLSGDDCILEREPLENLAA
                     DGQLKAFRHHGFFYAMDTYREYQYLNDLWSTGRAPWRLWS"
     gene            3708..4562
                     /locus_tag="VLE22_01320"
     CDS             3708..4562
                     /locus_tag="VLE22_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HSB13065.1"
                     /translation="MTSTSPRFGPSGWEFPQWLGVVYPKHLPRGFHPLAFLAQHFDAV
                     EIRSTFLRVPRPEIARLWLEKVSQNRNFAFSAVLHRRFTHERALDPAEAAVFKEGLRP
                     LLRAGRLGCLVMQFPWAFRFTAENREYLIELRRAFHEFPLVVEMRHNSWLADEALGTL
                     IDYRLGFVNIDQPEYARAMPPTSMVTSSVAYVRLHGRDPKYWDREFGRTVSAAGQPPA
                     GGDYLYSTGELDEWKKRIEHVSKYAAKTFVVTANDGGGKSVVNAMQLARVFASNAGRI
                     EMPHTAVA"
     gene            4626..4826
                     /locus_tag="VLE22_01325"
     CDS             4626..4826
                     /locus_tag="VLE22_01325"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HSB13066.1"
                     /translation="MTQKVIEIVGVSPVSFAKAAENAVQEAAKTVRNIKWAHVESLDM
                     ALDGGKVLQYRATTKIYFDVER"
     gene            complement(4854..6224)
                     /locus_tag="VLE22_01330"
     CDS             complement(4854..6224)
                     /locus_tag="VLE22_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB13067.1"
                     /translation="MVFACALSAQAAEGANPSLPSVLLITVDTLRADHLTCYGYQFNT
                     SPNIDRLANEGTRFDKAYTVVPLTGPAHFSLFTSKYPQEHGARRNGVSLAKGAVPVSF
                     PRILRKHGYRNAAFISGWPLTKRLTHLDRWFHYYDEELPRTYQLFNSSRYAEDVTPRA
                     LRWLRGRAGKKRPFFLWIHYFDPHEPYVFREAFRQRKANGDPTPAPLPWTNAAFEERV
                     RGYDSEIAYTDHHIGVLLSALDELKLRESTLVVLTADHGESLGEHDYVGHGRHLFENI
                     IRIPLVVRLPGAVPAGRVISSPVSILDIGPTILDLTVEKLPRYKRPPVLHSGHSFVAA
                     FRDGGRLPERTQYYVTFAGKKGFAPSWLSWLWVHDGELPLRFGRLKGDDKTVWSPGDD
                     SLEIYDVSADRYETRPIQLDPGNSEYGKETASLRWWFSATEFREGEDSLTSRDLEVLK
                     SLGYLQ"
     gene            complement(6313..7692)
                     /locus_tag="VLE22_01335"
     CDS             complement(6313..7692)
                     /locus_tag="VLE22_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB13068.1"
                     /translation="MSRRSFCSLLSASGLGSALDWRGAQLALRRPNLIILLADDLGYG
                     DLGCYGNRRMRTPNLDRMAAEGARLTEFYAAPTCTPARSSLLTGRYPLRSGLVRVLMP
                     HERFGLPDSEVTLAERLKDQGYSTACIGKWHLGDLPPFRPNRHGFDYSFGLLYSHDMR
                     LPVFQWPPLRLYSDGRALDLPLRFRELTQLYTREALHFIKRSRDNPFFLYLPYSLPHT
                     PLSASVNFRGKSTAGRYGDAVEEIDWSVGEILDALAADGLDNDTLVIFASDNGPDLSA
                     GGSAGILRGGKGTTWEGGIRVPAIFRWPGVIPPGVVRDGISSLMDLYTTLIELAGGDV
                     PSDRQVDGRNILPWLAGSAGSPNLGFFYYSGRHLFAFRSGPWKIHFRKFELGPGGARG
                     PVVSCRPPELYNLFLDPAESVNVAAQYPKMAARLAEETFDSGAVIEPGPLPPPRWRSL
                     LRILRARPV"
     gene            7819..8616
                     /locus_tag="VLE22_01340"
     CDS             7819..8616
                     /locus_tag="VLE22_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HSB13069.1"
                     /translation="MRYLVVGAHPDDCEYRFGGTTVKLAAQGHAVKFLSVTNGDAGHH
                     VQGGVTLVARREAEAQEGARRAGAAACESLDNHDGEYMPTLQNRCEIIRQIREWKADV
                     VITHRPNDYHPDHRYTSMLVQDAAFMVMVPNICPATPALRHNPVFLYMEDDFRKPAPF
                     SPDIVVDITDVWDQKVSSIGAHVSQFYEWLPWLDGDLDKVPTDASERKKWLDQWLRKL
                     LKNDFREAVERRYGKEQAGRVRLVEAFEICEYGRQPSPEERDKLLPR"
     gene            8733..11249
                     /locus_tag="VLE22_01345"
     CDS             8733..11249
                     /locus_tag="VLE22_01345"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HSB13070.1"
                     /translation="MRTTLPVVAVFASVFLPSPNKAGDLRSACSDAPKHQTEVVTSGK
                     HTYTVTMGGTIDGEMTRDPIGYWAYDQYWEPNLFVRIENIGDETAVNPWLLRKGRPDT
                     RTVQGIVGHVLEPSMSEAERARRLWEFEIKNRFHATTEDHEVDDVIKRFNCYGYTLCG
                     NESKIMSDLWRGAGLKTRRGFPNGHSTAEVYYDDAWHLLDSDESIICLLRDNKTIASE
                     EQIVRDHDLMKRTHSYGPLHDDNPLGDETSAALHYYEGERSGEQPSFTRHTMNFSLRP
                     GEAITWAWNPGNRFHGKEFEGKLANFWNKRWRLIANVMNGELSYTADLTRKSTLAHLA
                     SDGMELRESGPFGRALYVAGKSGSVVVPVKSAYPIIGGRLEVDFGRKDIRDEAVKTEI
                     SFDGGKTWKEVWTSAGSDYVRMYIDLDEFFPAADPARYEYLLRIAATSKLEQPAVCLK
                     GVSLRSKLQMARLAMPGVSLGDNAFTYVDESGPGRKVRITHAWNECNSAAAPQAPAGA
                     EYPPDGGKAEGTAITFRWQPPSGGTEPADYEFQLSEYADIRWVVSPNFHKLISRTAGR
                     GTASFKLPYKGLLNPGETYYWRVRARGDDGVWGPWSKVFSFSADAPAVPVKVETRFDR
                     GPRTATLNWEPGSGGTVPIRYRIYGSAERGFTANDSAYKYHAGVDGVLDSPPNLLLET
                     KGPVKSVALPAELWRPYYRVVAVDASGHESGASDKAELTHPLIRTRALTEARAGAYYE
                     AQVDLSASAGHLTSANLNGKPYHMKFRDGDEPSFELSGAPEGLNIDRRSGLIAGYLPA
                     KTAGSYTLSVTVTDGRSKQEDAVTFFLRVRGEEPVRRSAR"
     gene            11257..11883
                     /locus_tag="VLE22_01350"
     CDS             11257..11883
                     /locus_tag="VLE22_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HSB13071.1"
                     /translation="MTKLVRYSTFLILLPALAQQPLELPQPQTDGGRPLMQVLKDRKT
                     SREFSGESLPPQVLSNLLWAAFGINRPADGRRTAPSAHNWQEVDIYVATAGGAYLFEP
                     KTHSLKPVIADDIRKSTGTQAFVADAPVNLVYVADYTRMVDASESDKISYSAADTGFI
                     AQNVYLFCASEGLATVVRGGVDQSLPKLLKLRPDQHITLAQTVGYPKK"
     gene            complement(11897..12682)
                     /locus_tag="VLE22_01355"
     CDS             complement(11897..12682)
                     /locus_tag="VLE22_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSB13072.1"
                     /translation="MQPSPPLIEFRNITVRRGLRPALDGFSLSISLGEHVAVLGPNGS
                     GKSSLIKTITRECYPLPAPDSYVRILGEEVWNIFDLRALLGIVSTDLLESCTWDITGL
                     EMVLSGFFSSVGVWPNHHVTTLMEHKARELLHLLEVAHLAGRSIHEMSAGEVQRTLIG
                     RALVHGPKALVLDEPSNSLDVRARHELSGALRNLAAGGTSIVMVTHHLGDIIPEIGRV
                     VLIKHGKVFADDSKESVLTAGLLSRLFDTPVEVTRRDGYFHLW"
     gene            complement(12687..13847)
                     /locus_tag="VLE22_01360"
     CDS             complement(12687..13847)
                     /locus_tag="VLE22_01360"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HSB13073.1"
                     /translation="MPRKTLHAAMTFGLLAALSCGPGARKQTEEGAKLTKELFGKTAD
                     GQAVDIYTLRNKADVEVRITSYGGIVVSLKAPDRVDKLDDIVLGFDSLGGYLAEHPYF
                     GAIVGRYGNRIAKGRFTLNGVEYRLARNNGENALHGGIKGFDKAVWTAKETTVDGAPA
                     VEMRYLSKDGEEGYPGNLSATVTYSLNDRNELRIDYRATTDKDTVLNLTNHSYFNLAG
                     QGEGDILSHVMMINAARFTPVDAGLIPTGELRSVEGTPFDFRTPTAIGARIDGKDEQL
                     VRGKGYDHNFVLDGQAGTLSLAARVTEPKSGRILEVLTTEPGVQFYTGNFLDGTIHGK
                     GGRVYNLRYGFCLETQHFPDSPNQPAFPSTVLKPGAEYHTTTVYRFSAETSR"
     gene            13975..14967
                     /locus_tag="VLE22_01365"
     CDS             13975..14967
                     /locus_tag="VLE22_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSB13074.1"
                     /translation="MIRVAVTEVEYKKARMVFDSAEDMACIPVLPNEDDLCASIRKLQ
                     ARHAIVGAVRYEDLLYRTLPRGGVLARFGVGHDGIDKAKATASGILCVNTPGVLDESV
                     AEYVMALLLEAARHLVRHAETCQRGSWEVHIGRELSGKRLAVIGCGPIGRLVGRAAAQ
                     GFRMEVIGCEVAPVDTVELASLGFSRITTDFADAVAGADFVSLHIPGSPPNRHFLSRE
                     RLAMIPSRATLINTARGSVVDEIALFDALASGSLAGAALDVFEEEPYRPQDPAKDLRT
                     LPKVIQTPHVASSTREACDRVAKRALHNIALAERGELGSMDLLNPEVLTEREGA"
     gene            14964..15239
                     /locus_tag="VLE22_01370"
     CDS             14964..15239
                     /locus_tag="VLE22_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13075.1"
                     /translation="MKNTLWKALIEQRLTLGGWLHPACVEVVARRASTGCAWTRRMDL
                     SGSFGKTGDFDCTEMGDTLDTYKQACRRHRKSTGLYIIRPTQENRKT"
BASE COUNT         3148 a         4571 c         5229 g         2788 t
ORIGIN      
        1 gccgggagtc gtccaacgca aagtcgtttt ccgtggcggc gtggatcacg taggcgtact
       61 cgccttccgg aaaggaaaac gaggcgatct cgccagagtg cagccggacc gacggatgcc
      121 cggccaggtg cggcgccttc ttgcggaagg cctccggatt ccgtgtcaac acggtagccg
      181 aggcgccaag gccgagtttg tcgttcgccc aagtgaaaga ctcgagcagc cagcagccga
      241 agaacccggt gcctccggtg atgaagatcc gctggccacg cagctcatcc cagagtcctt
      301 gtgtgtggac gaggacgtga tcgagatctt cggctaacgg gttcatcgga gcgcgttctc
      361 catcgccact gggacacggt gaaacgtctc cagcatgtag ttcaccatct ccgcgctaat
      421 ccctgggtag acgcctaccc agaagaggtg gttcatgacg aaatcggagt tgcggaggtc
      481 gccggcgacg cggtgcggga tgtcgcggta ggccggctgg cgaagcaggt tgccgccaaa
      541 cagcagccgc gtggcgatct tgcgttcctc gagataacgg accacatcgg cccgcgaaaa
      601 cggcgcttcc ggacgcaccg ctaccgggaa gccaaaccag ctcggattcg agcccggcgt
      661 cgcccgcggc agcacgaaga attcctccag atcccgcagc ccttcacaga gcgcacggaa
      721 attgcgccgg cgcgcctcga taaagccggc cagcttgcgc aactgcgcca ggccgacggc
      781 cgcctgcata tcggtcgctt tcaagttgta gccgacgtgg gaaaagacgt acttgtggtc
      841 gtagccgtgg ggcagatcgc cgaactgcca gtcgaaacgc ttgccgcagg tgttggcctt
      901 gcccggctcg caccagcagt cgcggcccca gtcgcggaac gaggcggcga gcgtctgaag
      961 cagaggctgg tccgtaacca cgcagccacc ttcgcccatc gtgatgtggt gggcagggta
     1021 gaagctgaac gtcgccagat cgccgaacga gccgacccga cggccttcgt attccgcgcc
     1081 gagagcgtcg caacaatcct cgaggaacca caggccgtgc cggcgcgcga aggcggtcac
     1141 cgcggataca tcgaagggat tcccgagcgt gtgcgcgatc atgaccgcgc gcgtcttctc
     1201 ggagagcgcg tcatccaggc ggctcacgtc gatgttgtac gtgggaacgg tgatgtccac
     1261 gaaaaccggg acgaggcgat tctggacgat ggggttgacc gtggttggaa aaccggccgc
     1321 gatcgtaatc acttcgtcgc cgggcgtgag ccggcggtcg ccaagtttag gcgaggtcaa
     1381 agcggacagc gccaccaggt tcgccgagga accggagttg acgatcaggg cgtcgcggac
     1441 accgcagtag cgggcgaact gcttctcgaa ttcggcggcg aagcggccgg cggtaagcca
     1501 gaagtcaagc gcggaatcga caagcgactg catgtcgccg gcgtcgaaca ctctgccgga
     1561 gacgggcacg ggagagactc ctggaatgaa ctcccctcca gcgtgggcgg cctcgtgata
     1621 ctcggcgacc aggcccagaa tacggtctcg caactgccgg gcgcgctcgc tcacgacgaa
     1681 agaaactccc tgtaccaatg gattgtgcgc tccagaccct gatcgagagt aaagagcggc
     1741 gcccagttca acagacggcg ggcgcgttcc gcgcagaggt actgccggcg gatctcgttc
     1801 gaggcctcgt tgcggacatc cggaacgagg gccgaaccca tccgggtgag gatccgctcg
     1861 acgagttcga gcacggtcac ctgcgcctcg ttggagaagt tgaacgccga gcccgcgaac
     1921 tcggggctgc cgcggagctt ctcggcaagg agcatgtagg cggcggcgcc gtcctcaaca
     1981 tagaaataat cgcggacgaa ctggccgtcg gaacggatga cgggacgttc gccgcgcagc
     2041 acggaccgga tcgcgccggg cacgatgcgg ttccagttca ggtcgccacc accgtagaaa
     2101 ttgccgcaac gggtgatcgc aaccggcagg ccgtaggtgg ccgcgtaggt ctgggcaatc
     2161 aggtcggcgc aggacttgct ggcgtcgtag gggtggcgtc cctggagggg acagtcttcg
     2221 gtataaggaa gctgcttctg ttcgccgtag gccttgtcgg aagaagcgac gatgatggcg
     2281 tggactttcg gcgagcggcg gcaggcttcg agcacgttcc aggtcccctg gatgttgctc
     2341 tcgaacgtgg aaatggggtt gccgttggcg atgccgacga tggtttgggc cgcgaggtga
     2401 aagacggagt cgatttcgta ctcggcgagc gtgcgctcga gaacctcgcg gtcgcggaga
     2461 tcgccgcgga cgatcttgac acggtcgatg aggcgggcgc acacgagctc gctctggggg
     2521 acccagtcgc gcacgaggca aacgatgtcg gcgccctgtt cgaggagccg gcggacgagc
     2581 cagccgccga ggagtccggt ggcgccggtg acgaacacag gacggtcgcg ccagaatgtg
     2641 ccgttcagga ccatagtctc cagggggctc ggccggtgga ccacaggtcg ttcaagtatt
     2701 gatactcgcg ataggtgtcc attgcataga agaagccgtg atggcggaaa gccttgagct
     2761 ggccgtcggc ggcgaggttt tcgagcggct cgcgctcgag gatgcagtcg tcgccgctga
     2821 ggtagtcgaa gatgccgggt tcgaagacaa agaagccggc gctgatccag ccgtcggagc
     2881 gaggcttttc actgaactgc ttgaccgaac cctcctcatc cagggcgacg ctgccgaagc
     2941 gagagaccgg gcggacggta gtgacggtgg ccaaccggcc gtggctcttg tggaagtcga
     3001 tcaggcggcc gatgtccagg tcggccagac catccccgta tgtgaccaga aacgtgcccc
     3061 catccaggta cttctggacc cgccggatgc gccctccggt catggaatcg aggccggtgt
     3121 cggctaaggt cacccggaag tcctgctcgg tatgcacgcc gttgtaatgg atctgggacc
     3181 gttggccgag gcagatggtg aagtcattgt tcatcgcctc gtagttgaga aagtactcct
     3241 taatcatgtt gccgcggtag ccgaggcaga ggacgaagtc acggaagcca tggtgggcgt
     3301 acatcttcat gatgtgccag aggatggggc ggccgccgat ttcgaccatg ggcttcgggc
     3361 ggaattccgt ctcctcccgg agccgggtgc cgagacctcc gcaaagcatg accactttca
     3421 taaggcggcg tcctgcctgt gcaatcaggt gctttccccc cggagccggg tacgcgtccc
     3481 gggcgacggt ttacgactca cggctgttaa ccatccgtac taaccttcca cgaatctacc
     3541 atttgtttcc aatgcggtaa atactggatt gagataacgg ggagcgatct actttcgata
     3601 acagcggcat gcgcgggacc ccgcggttcc catcatttag aaagatcttg acctgagggg
     3661 cacgaatgcg ctatcctggg cgaagaaaag gcgaaatagg agctgttatg acgtccacct
     3721 ccccccgttt tggaccgtcg ggctgggaat tcccacagtg gctgggtgtg gtgtatccga
     3781 aacatctgcc gcgagggttc cacccgctgg cgtttctggc acaacacttt gacgcggtgg
     3841 agatccggag cacgttcctc cgggtgccgc ggcccgagat cgcacgcctg tggctggaga
     3901 aggtgagcca gaaccggaat ttcgcgttct ccgcggtgct gcaccgccgg ttcacgcacg
     3961 agcgagccct cgatccggcc gaggcggcgg tcttcaagga agggctgagg cccctgttgc
     4021 gggccgggcg gctcggctgc ctggtgatgc agttcccgtg ggcgttccgc tttacggccg
     4081 agaaccggga gtacctgatc gagcttcgcc gcgcgttcca cgagttcccg ctggtggtgg
     4141 agatgcggca caacagttgg ctggcggacg aggcgctcgg cactctgatc gactaccggc
     4201 tgggcttcgt gaatatcgat cagcccgagt acgcacgggc gatgccgccg acgtcaatgg
     4261 tgacgtcttc ggtggcgtac gtccggcttc acgggcgcga tccgaaatac tgggaccggg
     4321 agttcggaag gacggtctcc gctgccggcc agccgccggc cggcggcgac tacctgtaca
     4381 gcactggcga actcgacgag tggaagaagc ggattgaaca cgtctcaaaa tacgcggcca
     4441 aaacgttcgt cgtgacggcc aatgatggcg gcggcaagtc cgtggtcaac gcgatgcaat
     4501 tggccagggt gttcgcgagc aacgccggcc gaatcgaaat gccccacacg gcggtcgcat
     4561 gatccgccgg cacgggcggt ccgttgaccc tttgttatac ttgtctgaac ctcaggagga
     4621 acaagatgac gcagaaagta attgagattg tcggagtttc gccggtgagt tttgccaagg
     4681 cggcggagaa cgccgtgcag gaggccgcga agacggtccg caacatcaag tgggcacacg
     4741 tcgaatccct ggacatggct ctggacggcg gcaaagtcct ccagtatagg gccaccacta
     4801 agatctactt cgacgtcgaa cggtaggaga aaacgggcga acggccccgc ctgctactga
     4861 agatagccca ggcttttcag aacctcaagg tccctcgagg tgaggctgtc ctcgccttcg
     4921 cggaattcag tcgcggaaaa ccaccagcga agggaggcgg tttccttccc atattccgag
     4981 ttgcccggat ccaactggat tgggcgggtt tcgtaccggt cagctgaaac gtcgtagatc
     5041 tcgagcgaat cgtctccggg cgaccagacc gtcttgtcgt cacctttgag gcggccgaat
     5101 cgcaacggca gttccccgtc gtgcacccac agccaggaga gccaggaagg cgcgaaacct
     5161 tttttccccg cgaaagtgac gtagtactgc gtccgctccg gcaagcgacc gccgtcacgg
     5221 aaagccgcga cgaaactgtg accggagtga aggacaggag gccgcttgta ccgcgggagc
     5281 ttctccacgg tgagatcgag gatcgtcggg cctatatcca gaatcgagac cggcgaggag
     5341 atcaccctgc ctgccgggac tgcgccgggg agccgtacaa cgagcgggat gcggatgatg
     5401 ttctcgaaca ggtgccgccc gtgcccgacg tagtcgtgtt cacccagact ttcaccgtgg
     5461 tcggcggtga ggaccaccag ggtggactca cgcagtttca gctcgtccag ggcggagagc
     5521 agaacgccaa tgtggtggtc ggtataagca atttccgagt cgtagccgcg gacgcgctcc
     5581 tcgaacgcgg cgtttgtcca agggagcggc gccggtgtgg gatccccgtt ggccttccgt
     5641 tgtcggaatg cctcacgaaa aacgtaaggt tcatgcggat caaagtagtg aatccacagg
     5701 aaaaagggcc gtttctttcc ggcacggcca cgcagccatc ggagggcgcg tggggttacg
     5761 tcttcggcat accggctgga gttgaagagc tggtaggtgc gcggcagttc ttcgtcgtag
     5821 taatgaaacc agcggtccag gtgggtgagg cgcttcgtca gcggccagcc actgataaag
     5881 gcggcgttgc ggtagccgtg ctttcgcaga atccggggga acgagacagg caccgcgcct
     5941 ttcgcgagac tgactccatt cctgcgcgcg ccgtgctctt gggggtactt gctggtgaat
     6001 agcgagaaat gcgccgggcc ggttaacggg acaaccgtat aggccttatc gaaacgcgtt
     6061 ccttcgttcg caagacggtc gatattcggg ctggtgttga attggtagcc gtagcaggtg
     6121 aggtggtcgg cccgaagagt gtcgacggta atcagcagga cgctcggaag gctgggattc
     6181 gctccttcgg cggcttgcgc cgagagcgca cacgcaaaaa ccaaaattgc gacgccccag
     6241 ttcaacctga aagcatttta atcccattct ttggcactgt ccatcccgtc aacggggtag
     6301 tcacgaggcc tctcaaacgg gccgcgcccg aaggatacgc aagagggacc gccatcgcgg
     6361 gggcggcaac ggaccaggtt cgatgactgc cccggaatca aacgtctctt ccgccagacg
     6421 cgcagccatc ttggggtatt gggccgcaac attgactgac tcagcaggat caaggaagag
     6481 gttgtacaac tctggaggcc tgcaggacac cactggtcca cgggcaccgc ccggaccgag
     6541 ttcgaatttt cggaaatgga tcttccaagg gcccgagcga aaggcaaaga gatgcctgcc
     6601 gctgtagtag aagaacccga ggttgggaga acccgcgcta ccggccagcc atggaaggat
     6661 gttccggcca tcgacttgcc ggtcgctggg gacatcgccg ccggcaagtt caatgagtgt
     6721 tgtgtaaagg tccatgagcg aggagattcc gtcccggacg acaccgggag gaatcacgcc
     6781 cggccatcgg aagattgcgg gcacgcggat ccctccctcc caggttgttc ctttcccgcc
     6841 acgcaagatg ccggcgcttc cacccgccga caagtccggg ccgttgtcgc tggcgaagat
     6901 cacaagcgtg tcgttgtcca atccgtcggc ggcgagagca tcgaggatct cacccacgct
     6961 ccagtcgatc tcttccacag cgtcgccgta gcggccggcg gtggatttgc cgcggaagtt
     7021 cacggatgcg gacaggggcg tgtgtgggag cgaatagggc aggtatagga aaaaaggatt
     7081 gtcccggctg cgcttgatga agtggagtgc ctccctggtg tacaactggg tgagctcgcg
     7141 gaaacgaagc ggcaagtcca gagctcggcc gtcgctgtac aagcggagtg gaggccactg
     7201 gaacacggga agcctcatat cgtggctgta gaggaggccg aaggagtagt cgaagccgtg
     7261 ccgattcgga cggaacgggg gcaggtcacc gaggtgccac ttcccgatgc aggcggtgga
     7321 gtagccctga tccttgaggc gctccgcgag ggtcacctct gaatcgggca acccgaacct
     7381 ctcgtgcggc atcagcactc gaacgagtcc ggaacggagc ggataacgtc ccgtgaggag
     7441 cgatgagcgg gccggggtac aagtgggcgc cgcgtagaat tcggtgagcc gggcgccttc
     7501 ggcagccatt cgatcgaggt tgggcgtcct cattcgacgg ttgccgtaac agcctaagtc
     7561 gccgtagccg aggtcgtcag cgagcagaat gatcaggttc ggacgccgta gggcgagttg
     7621 cgcaccccgc caatcgagtg cactccccag cccgctggca gacagaagac tgcaaaatga
     7681 gcgtctgctc aacactactt ccttttgtac aatgaccgga accacggaac aacccggatg
     7741 agtcgcatcg ccccgggatc ggctatgctt tcaagtttgg gcgtttgaag cccaactgga
     7801 tcgggaggga gcagcggcat gcggtatctg gttgtcgggg cgcaccccga cgattgcgag
     7861 taccggttcg gcgggacgac ggtcaaactc gcggcgcagg ggcatgcggt gaagtttctg
     7921 tcggtgacca acggggatgc cggccaccac gtccagggcg gcgtcacgct ggtcgcgcgg
     7981 cgcgaggccg aagcgcaaga gggagcgcgg cgggccgggg cggcggcgtg cgagtcgctg
     8041 gacaaccacg acggcgaata catgccaacg ctccagaacc gctgcgagat catcaggcag
     8101 atccgcgagt ggaaggcgga cgtcgtgatc acgcaccgtc ctaacgacta ccaccccgac
     8161 caccggtaca cgagcatgct ggtgcaagac gccgcgttca tggtgatggt gccgaacata
     8221 tgcccggcga cccccgcgct tcggcacaat ccggtcttcc tgtacatgga agacgatttc
     8281 cggaagccgg cgccgttttc gcccgacatc gtggtggaca tcaccgacgt gtgggaccaa
     8341 aaggtgagtt ccatcggcgc gcatgtctct cagttttacg agtggctgcc gtggctcgac
     8401 ggcgatctcg ataaggttcc cacggacgcc tcggaacgaa agaagtggct ggaccaatgg
     8461 ctccggaaac tgctgaagaa cgacttccgc gaggcggtgg agcggcgcta cggtaaggag
     8521 caggccggcc gggtgaggct tgtcgaggcg ttcgagattt gcgaatacgg ccgccagcct
     8581 tctccggaag aacgggacaa actactcccg cggtaaggcg ggatgacgcg ggaccgccgt
     8641 ccgcggtatc atccgacttc aacgggggta ctggataacc cgcggcacag ccaaggccgt
     8701 gccgcgctaa agcacccggc taagaggcgg tcatgcgaac gacactaccg gtggttgctg
     8761 tatttgcgtc tgttttcctc ccaagtccca ataaggcggg tgatttgcgg agcgcctgct
     8821 ccgacgcacc gaagcatcaa accgaggtgg tcacctcggg caaacatacg tacacggtaa
     8881 cgatgggcgg gactatcgac ggcgagatga cgcgcgaccc gatcggctac tgggcctacg
     8941 accagtactg ggaaccgaat cttttcgtgc ggatcgaaaa catcggcgac gaaacggccg
     9001 tcaacccgtg gctcctgcgt aagggtaggc cggatacgcg aacggttcag ggcatcgtcg
     9061 gtcacgtgtt ggaaccatcg atgagcgagg ccgagagagc tcgccgtctg tgggagttcg
     9121 agatcaagaa ccggtttcac gcgaccaccg aggaccacga ggtggacgac gtcatcaagc
     9181 gcttcaactg ttacgggtac acgctctgcg ggaacgagag caagatcatg agcgacctgt
     9241 ggcgtggcgc ggggttgaag acgcgccgcg gcttcccgaa cgggcacagc acggccgaag
     9301 tctactatga cgatgcgtgg cacctgctcg attccgacga aagtatcatc tgcctgctgc
     9361 gggacaacaa gacgatcgcc tccgaagagc agatcgtgcg cgaccacgac ctgatgaagc
     9421 gcacgcactc ctacgggccg ctacacgacg acaacccgct gggggatgag acgtcggccg
     9481 cgctgcacta ctacgaaggc gagcgcagcg gtgaacagcc gagcttcacc cggcacacga
     9541 tgaacttctc gctgcgtccc ggcgaggcga tcacgtgggc ctggaacccg ggaaatcgtt
     9601 tccacggcaa ggaattcgaa ggcaagctgg cgaacttctg gaacaagcgc tggcgcctga
     9661 tcgccaacgt gatgaacggg gagctgagtt acacggctga tctgacgcgg aagtccacgc
     9721 tggcgcatct ggcgagcgac ggaatggaac tgcgcgagag tggaccgttc ggcagggctc
     9781 tgtacgtggc gggcaagtca ggatcggtgg ttgtaccggt aaagagcgcg tatccgatta
     9841 ttggtggccg tctggaggtt gacttcgggc ggaaagacat tcgtgacgag gcggtgaaga
     9901 ctgagatcag ctttgacggc ggcaaaacat ggaaagaagt gtggaccagc gcgggcagcg
     9961 attacgtacg catgtacatc gacctcgatg agttctttcc ggcggcggac ccagcccggt
    10021 acgagtacct gctgcgcatc gcggcgacaa gcaaactcga gcagccggcg gtctgtctca
    10081 agggagtgtc cctgcgttcg aagttgcaga tggcgaggct ggcgatgccg ggcgtgagtc
    10141 tgggagacaa cgccttcacg tacgtcgatg agagcgggcc cgggcgaaag gtacggatca
    10201 cgcacgcctg gaacgagtgc aactccgcgg cggcgcctca ggctccagcg ggcgcggagt
    10261 atccaccgga cggaggcaag gcagagggca ccgcgatcac gtttcgttgg cagcctcctt
    10321 cgggcggtac ggaaccggcg gactacgagt ttcaattgag cgaatacgcg gacatacggt
    10381 gggtcgtgtc gccgaacttt cacaagctga tcagccggac ggccggccgc ggcaccgcgt
    10441 ccttcaagct gccgtacaag gggcttctga atccgggcga aacgtattac tggcgggtgc
    10501 gcgcccgggg cgacgacggg gtttgggggc cgtggtccaa ggtattctcg ttctcggcgg
    10561 acgcgccggc agtgccggtg aaagtggaga cgcgtttcga ccgtggtccc cgcacggcca
    10621 cgctgaattg ggagccgggt agcggcggga ccgtaccgat tcggtaccgg atttacggaa
    10681 gtgcggagcg aggtttcacc gcgaacgaca gcgcctacaa gtatcacgcc ggagtagacg
    10741 gtgtactcga cagtcctccg aacctgttgc ttgagacgaa gggtccggtg aagtcggtag
    10801 cgctaccggc cgaattgtgg cggccgtact atcgcgtggt ggcggtcgac gcctccggcc
    10861 atgagagcgg ggcgtcggat aaggcggagc ttacacaccc gctgatccga acgcgcgccc
    10921 tcaccgaagc ccgcgccggc gcgtactacg aagctcaagt ggatctttcc gcatccgccg
    10981 gccacctcac gtcggcgaac ttgaacggca aaccgtacca catgaagttc cgcgacggcg
    11041 acgaaccatc atttgaactg tcgggcgcgc cggagggact caatatcgac cgaaggagcg
    11101 gcctgatcgc gggctatctg cccgccaaga ctgccggctc atacacactg tccgtcacgg
    11161 tgacggacgg tcgcagcaag caagaggacg cggtgacgtt cttcttgcgg gttcgaggtg
    11221 aggaacccgt tcgccgttcc gcgaggtgaa actgccatga caaaactcgt ccggtattcg
    11281 acattcctga tcctacttcc cgctcttgcg cagcaacccc tcgaactgcc gcagccgcaa
    11341 acggacggag gccggccgct aatgcaggtt ctaaaagacc ggaagacgtc ccgggagttc
    11401 agcggcgaaa gtctgccgcc gcaggtgcta tccaaccttc tgtgggcggc gttcggcatc
    11461 aaccgcccgg cggatggtcg gaggacggcg ccgtccgcgc acaactggca ggaggtcgac
    11521 atttacgtcg caacggcagg cggagcctac ctgtttgagc cgaagacgca ctcgctcaaa
    11581 cccgtcattg cagacgacat ccggaagagc acgggtacac aggcgtttgt ggccgacgcc
    11641 ccggtcaatc tggtgtacgt tgcggactac actaggatgg ttgacgcgag cgaatcggac
    11701 aaaatatcgt actctgccgc ggatacgggg ttcatcgccc agaatgtgta tctgttttgc
    11761 gcttcggagg gactggccac ggtagtccgc ggcggcgtag accagtcgtt gccgaagctt
    11821 ctaaagctgc ggccggatca gcacatcact ctggcgcaga cggtggggta tccgaagaag
    11881 tagggtccga gcgcaactac cagaggtgaa aatagccgtc ccgccgggtg acttcgaccg
    11941 gggtgtcgaa gaggcgggac agcagtcccg cggtcaggac gctctccttg gaatcgtcgg
    12001 cgaacacttt gccgtgcttg atcaggacga cacggccgat ctccgggatg atgtcgccga
    12061 ggtggtgcgt caccatgacg atgctcgtgc cgccggcagc cagattgcgc agagcgccgc
    12121 tcaattcgtg gcgcgcacgg acgtcgaggc tgttgctggg ttcgtcgagc acgagagcct
    12181 tcgggccgtg gaccagcgcc ctgccgatca gcgttcgttg cacttcaccg gcggacattt
    12241 catggatgct gcggccggcg aggtgagcaa cctccagaag gtggagcagc tcccgggcct
    12301 tgtgttccat caacgtcgtg acgtgatgat tcggccagac gccgacgctg ctgaagaagc
    12361 cggagagtac catctcgagt cccgtgatgt cccaagtgca ggattccagc aggtcggtcg
    12421 aaacgatccc caggagcgcg cgaaggtcga agatgttcca aacctcctcg ccgaggatgc
    12481 ggacatagga gtcgggcgcc ggcagcggat aacactcgcg ggtaatggtc ttgatcaaag
    12541 acgacttgcc cgagccgttc ggcccgagca ccgcaacatg ctcgccgaga gagatggaaa
    12601 gagaaaagcc gtcgagtgcg gggcgcagac cgcgccgaac ggtgatgttg cggaactcga
    12661 tcagcggcgg cgaaggctgc atgcagttat cgcgacgtct ccgcggagaa gcggtagacc
    12721 gtggttgtat ggtactcggc tcccggcttg aggacagtcg agggaaacgc cggctggttc
    12781 ggagagtccg ggaaatgctg ggtctcgagg cagaaaccgt agcggaggtt gtacactctg
    12841 ccacctttgc cgtgaatcgt accgtcgaga aagttgccgg tgtaaaactg cacgccgggc
    12901 tcggtggtca gcacttcgag aatacgccct gatttgggct ccgtcaccct cgccgcaagt
    12961 gagagcgtcc cggcttggcc gtcgagcacg aaattgtggt cgtagccctt tccgcgtacg
    13021 agttgttcgt ctttgccgtc gatccgcgcg ccgatcgcgg tgggtgtgcg gaagtcgaag
    13081 ggcgttccct cgaccgagcg cagttcgccc gtgggaatca ggccggcatc caccggtgtg
    13141 aaccgggcgg cgttgatcat catgacatgg ctcagaatgt caccctcgcc ctgtcccgca
    13201 agattgaagt aggagtgatt cgtcaggttg aggaccgtgt ccttgtcggt ggtggcgcgg
    13261 tagtcgattc gcagctcgtt gcggtcgttc agcgagtaag tcacggtggc cgacaggttg
    13321 ccgggataac cctcctcccc gtccttgctg aggtacctca tctcgacggc gggagccccg
    13381 tccacggttg tttccttcgc tgtccagacg gccttgtcga atcccttgat gccgccgtgc
    13441 agggcgtttt caccgttgtt tcgcgcgagt ctgtactcga ctccgttcag agtgaatctg
    13501 cctttggcga tgcggttgcc gtagcgcccg acaatggcgc cgaaataggg atgctcagcg
    13561 aggtagccgc cgagggaatc gaagccgagg acgatgtcgt cgagtttgtc tacgcggtcc
    13621 ggcgccttga gagaaacgac gataccgccg tagctcgtga tcctgacctc gacgtcggcc
    13681 ttgttccgta gcgtgtagat gtctacggct tggccgtcgg ccgtcttccc gaacaattct
    13741 ttcgtcaatt tggctccttc ctcggtctgc ttcctggctc ccggtccaca cgagagggcg
    13801 gccaaaaggc cgaacgtcat cgcggcgtgc agtgtcttgc gcggcatagg ctgggccacc
    13861 gaaagcccgg gcggcaaggc aacaagatac cacacgggcg tggccggccc agagacgccg
    13921 atgtttcggt aagctggacg ctccggccca attaacaggg cggggctacg cacaatgatt
    13981 cgagtcgcgg tcaccgaagt tgaatacaag aaggccagga tggtgtttga tagcgctgaa
    14041 gacatggctt gcattccggt gctgccgaac gaagacgatc tctgcgcttc cattcgtaag
    14101 cttcaggcac ggcacgcaat cgtcggcgcg gtccgttacg aggaccttct gtaccgtacc
    14161 ttgccgcgcg ggggtgttct cgcccggttt ggcgtgggtc acgacggcat cgacaaggcg
    14221 aaggcgacag cctcggggat actctgcgta aacacgccgg gtgtactgga tgagtccgtc
    14281 gcggagtacg tcatggcgct gctgctcgaa gcggcgcgcc acctcgtgcg gcatgccgag
    14341 acgtgccagc gagggtcgtg ggaggtgcat atcggcagag aactaagcgg gaagcgcctg
    14401 gcggtgatcg gctgcggacc gatcggacga ctggtgggac gcgctgccgc gcaaggattc
    14461 cggatggagg tcatcggctg cgaggtcgct ccggtggaca ccgtagaact ggcgtcactc
    14521 gggttcagcc ggatcaccac tgatttcgcg gacgcagttg caggcgcgga tttcgtcagc
    14581 cttcacattc cggggtcacc gccgaaccgg cactttctta gccgggaacg gctcgcgatg
    14641 attccttccc gggcgaccct catcaacacc gcccgcggct ccgtggtgga cgagatcgct
    14701 ttgttcgacg cgctggcaag cggttcgctt gcgggcgccg cactcgacgt gttcgaggaa
    14761 gaaccgtacc gcccgcagga tccggcgaag gacttgcgga ctcttccgaa ggtgattcag
    14821 acgccgcacg tggccagcag cacccgtgag gcatgcgacc gtgtggcgaa gcgggcactg
    14881 cacaacatcg cgctcgccga gcgaggggag ctcgggagca tggacctatt gaatccggaa
    14941 gtactaacag aaagggaagg cgcgtgaaga acacactttg gaaggcgctg atcgagcagc
    15001 gtctcaccct tggcggatgg cttcacccgg cttgcgtgga ggtcgtggcc aggcgcgctt
    15061 cgaccgggtg tgcgtggaca cggaggatgg acttgagcgg atcgtttggg aagaccggag
    15121 attttgactg cacggagatg ggagacacgc tcgatacgta caaacaggcc tgccgtcgcc
    15181 accgaaagag taccggcctg tacatcatcc ggccaacgca ggaaaacagg aaaacatgaa
    15241 ggccgcaatt gatgaagact acaccctgat tgcgctcggt gcagatattg cgttcctagc
    15301 cgatagcgcg aaacaggcgc tccggcgggc tcagacttga atgccaaact gacacccggc
    15361 accgccgttc tgagagaacc ggtagagact cccgtccttt cggcgctcaa gtcccctgtt
    15421 cgccggatgg gcatataacc cccaaaacac gtattgtgaa aggaagggag ctttcgcctc
    15481 gcgcgatcgg ctctgtcccc agagtcctgg agcgatgtca ttgccgaata ctcccccact
    15541 cagagaaagc ttcggaggct gaacctgtga tcacgctcga atacaccgaa ggcagacaac
    15601 gggctgtcgt ggaagccgtc tctccggaag tcgattccgg tcgattccca atcaagagaa
    15661 cggtcggtga gcaggtgatg gtggaggcgg acgtgtacgg tgacgggcac gactccgttt
    15721 gctgcgttct cctctt
//
LOCUS       DATIFQ010000034         3045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1542424, whole genome shotgun sequence.
ACCESSION   DATIFQ010000034 DATIFQ010000000
VERSION     DATIFQ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542424"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VLE22_01375"
     CDS             <1..411
                     /locus_tag="VLE22_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13076.1"
                     /translation="SYSVPFSSPGSFASKVLEKDSPVRLIVPAGVTLAGDGLEPLGQE
                     PSTQASIFGVKGQEFKVEIQGTGVLQRPAEGEDESGPGFEQILPRIYDRVEWIVGLSL
                     AILILGLFLLYRRTETPATAARPEATAHKGKRLG"
     gene            408..1106
                     /gene="ccmA"
                     /locus_tag="VLE22_01380"
     CDS             408..1106
                     /gene="ccmA"
                     /locus_tag="VLE22_01380"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HSB13077.1"
                     /translation="MSAVSAEGVWKFYGDFPALRDITFQVEPGACLALLGRNGAGKTT
                     LLRILAGLLRPARGAISIFGEDARRRETRRRIGVLGHGIAIYDELSAFENLRLFGRLY
                     GLPDPRKSAMEWLERTGLERVKDGLVREFSRGMRQRLAVARAFLHDPGILLLDEPFTA
                     LDDRAIAILQTLLRQALADGHTIVTSTHQLREALELATQVALINRGRLAYYGARTAEM
                     LADPGWLYRNYGET"
     gene            1109..1789
                     /locus_tag="VLE22_01385"
     CDS             1109..1789
                     /locus_tag="VLE22_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HSB13078.1"
                     /translation="MLFLRQSLTIAAKDLRAELRTKEAINASVSFALVILLLFSFAFD
                     PTADQTKEMAGGLLWLDFAFAGALILNRSFARELTNDCLDALIASPVSGAALFLGKCL
                     ANLALLLAVELVSLPVFGVFYNVHWTQQFWPMMLVLLLATWGMTVIGTMFSAMTVNLR
                     LRELMLPTLVYPMIIPLLMSAMQLTTVLLAGQSIVGDNQIWLRLLVGFDIIFTALALG
                     LVETVLVG"
     gene            1813..2502
                     /gene="ccsA"
                     /locus_tag="VLE22_01390"
     CDS             1813..2502
                     /gene="ccsA"
                     /locus_tag="VLE22_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSB13079.1"
                     /translation="MMRQKVMIALCGLAAILLARNLYVILLKLPDEAAQGAIYRIIFF
                     HLPSWFTSFTAYFLAGVASILYLVKKQDGYDTFAVSVTEIGTVFTAMGLITGMIWARI
                     IWGIWWTWDPRLTWAFICWLIYAGYLMLRRAIEDPTARARNSAVLNIFSFTTVVITYK
                     AIDWWRTQHPAAVLSFRTGGGKIDPAMEAMIFWNWFALMLFAAVLVMIRMHQEQAQRE
                     LDALRRHAHAL"
     gene            2522..2677
                     /locus_tag="VLE22_01395"
     CDS             2522..2677
                     /locus_tag="VLE22_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HSB13080.1"
                     /translation="MDERNFTYMFYGLTAAWLVLVGYVVSLAGRENRLRKEIERLKHM
                     IEDREKK"
     gene            2677..>3045
                     /locus_tag="VLE22_01400"
     CDS             2677..>3045
                     /locus_tag="VLE22_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13081.1"
                     /translation="MDRRKLLQLAAGSGLAGVVSPRELAAQARVERATRGMPSPIIKD
                     VSVIATAPAGLRLAVVKITTDQDGLYGYGCATYTQRADLIVPAVERYLKPFLVGKPAD
                     RIDDTWQACYNSSYWRNGPVL"
BASE COUNT          530 a          937 c          976 g          602 t
ORIGIN      
        1 tcctattcgg ttccgttcag cagtcctggt tcgttcgcct cgaaggtgct cgaaaaggat
       61 tctcccgttc gattgatcgt cccggcggga gtgacgctcg cgggcgacgg actggagcct
      121 ctcggacagg aaccgagcac acaggcgtcg atcttcggag tgaaggggca agagttcaaa
      181 gttgagatcc agggaaccgg ggtgctccaa cggccagctg aaggcgaaga cgagtccggg
      241 cccggattcg aacagatcct cccgcggatc tacgaccggg tcgagtggat cgttggtctc
      301 agcctagcca tcctgatcct cggtctcttc ctcctgtacc ggcggaccga gacccccgcc
      361 accgcagctc gacccgaagc caccgcccac aaaggaaagc ggctgggatg agcgccgttt
      421 cggcggaggg cgtctggaaa ttctacggcg actttccggc tctccgcgac attaccttcc
      481 aggtggagcc cggcgcctgc ctggcgctcc tgggccggaa cggcgcaggt aagacaacgc
      541 tgctgcggat tctcgccggc ctgttgcgac ccgcccgtgg cgccatttcc atcttcggcg
      601 aggacgcccg gcggcgcgaa acgcgtcgtc gcatcggcgt cctcggccac ggcatcgcca
      661 tttacgacga gctttccgcc ttcgagaacc ttcgattgtt cggccggctc tacgggttgc
      721 cggacccccg aaagtcggcg atggaatggc tggagcggac cggcctcgag cgggtcaagg
      781 acgggctcgt gcgggagttc tcgcggggca tgcggcagcg cctggctgtc gcccgggcct
      841 tcctgcacga ccctggaatt ttgctcctgg atgaaccgtt caccgcgctc gacgaccggg
      901 caatcgcgat ccttcagacc ttgctgcggc aggcgctggc agacggccac acgatcgtca
      961 cttcgacgca ccagttgcgc gaggcgctgg aactggcaac acaggtggcg ctgatcaacc
     1021 gcgggcggct tgcgtactat ggcgcgcgga cagcggagat gctggccgac cccggctggc
     1081 tctaccggaa ctacggggaa acctgaggat gctgttcctg cgccagtccc ttacgatcgc
     1141 agccaaagac ctgcgggccg aactgcgcac caaggaggcg atcaacgcct ccgtctcgtt
     1201 cgcgctggtg attctgctgc tatttagctt cgcattcgat cccacggccg accagacgaa
     1261 ggagatggcc ggggggcttc tctggctgga cttcgctttc gcgggcgcac tcatcctgaa
     1321 ccgcagcttc gcccgcgagc tgaccaacga ctgcctggac gcgctgatcg cgtctccggt
     1381 ctcgggcgcc gcgctgttcc tcggcaagtg cctggcaaat ctcgctctgc tgcttgcggt
     1441 tgagcttgta tcgctgccgg tcttcggcgt cttctacaac gtacactgga cacagcagtt
     1501 ctggccgatg atgctggtgc tattgctcgc gacctggggc atgacggtga tcggaacgat
     1561 gttcagcgcc atgaccgtca atcttcggct gcgcgagttg atgctgccga ccctggtcta
     1621 cccgatgatc attcctttgc tgatgtcggc aatgcagctt acaacggtgc tgctcgcggg
     1681 ccagtcgatt gttggcgaca accagatctg gctccggctt ctcgtggggt ttgatatcat
     1741 ctttaccgcc ttggccctgg gcctggtgga gacggtcttg gtgggctgag taacagctca
     1801 aagaaggggg caatgatgcg acagaaggtc atgatcgcgt tgtgcggcct ggccgccatc
     1861 ctgctggcgc gaaatctata cgtcattcta ttgaaactgc cggacgaggc cgcgcaggga
     1921 gcaatttacc ggatcatctt tttccacctg ccatcgtggt tcacgtcctt caccgcctac
     1981 ttcctcgcag gcgtagccag catactgtac ctggtgaaga agcaagacgg ttacgacacg
     2041 ttcgccgtgt cggtgaccga gatcggtacg gtgttcaccg ccatgggtct catcacgggc
     2101 atgatctggg cgcgcattat ctggggcatc tggtggacat gggacccccg gctcacatgg
     2161 gcgttcatct gctggctgat ctatgcgggt tacctgatgc tccggagggc aatcgaggat
     2221 ccgaccgccc gggcgcggaa ttccgcggta ctgaacatct tctcgttcac cacggtggtg
     2281 atcacctaca aggcgatcga ctggtggcgt acccagcatc cggctgcggt gctcagcttt
     2341 cgcacgggtg gcggcaagat cgatcccgcc atggaagcca tgatcttctg gaactggttc
     2401 gcgttgatgc tgtttgccgc cgtactggtg atgatccgta tgcaccagga gcaggctcaa
     2461 cgcgagctgg acgcattgcg gcggcacgca cacgccctct agcagagtgg agaatcttca
     2521 gatggacgag cgcaatttca cgtatatgtt ctacgggctc acggccgcgt ggctggtgct
     2581 ggtcggttac gtggtgtctc ttgccggccg ggagaacagg ctccgcaaag agatcgagcg
     2641 gctaaagcac atgatcgagg acagggagaa aaagtgatgg atcgccggaa gctgctgcaa
     2701 ctggcggcgg ggagcgggct ggcgggcgtg gtctcgccgc gtgaacttgc cgcacaggcg
     2761 cgggtcgagc gcgcaacccg cgggatgccg tcgccgatca tcaaggacgt cagcgtcatc
     2821 gcgaccgcac ccgccgggct gcgtcttgcc gtggtgaaga tcacgaccga ccaggacggg
     2881 ctgtacggct acggctgcgc cacgtacacc cagcgtgccg acctcattgt gccggcggtc
     2941 gagcggtacc tgaaaccgtt cctggtaggc aagccggccg accggatcga cgacacctgg
     3001 caggcctgtt acaacagttc ctactggcgc aacggcccgg tgctg
//
LOCUS       DATIFQ010000035         4099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2117464, whole genome shotgun sequence.
ACCESSION   DATIFQ010000035 DATIFQ010000000
VERSION     DATIFQ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2117464"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..3802
                     /locus_tag="VLE22_01405"
     CDS             116..3802
                     /locus_tag="VLE22_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HSB13082.1"
                     /translation="MRRTPPSNSKKKRHDVSSANAVRPTTYETAEREAASFPIVGIGA
                     SAGGFEALMQMLQDLPPDTGMAFVFVQHMDPEHKSMLPKLLSKATQMPVVEAQQGMVV
                     QPNHVYVIPPKSDLGVINGVLQILSRRKTGGRYLPVDYFLCSLAEDQGGRAIGVILSG
                     TASDGTVGLNAIKAEGGITFAQDDESSKYYGMPGSAIASGCVDFVLPPNRIGKELARL
                     ARHPYIGLPHPAKMPELLPAGEDDFRRILHLLRTACGVDFSDYKPGTIRRRIARRMAL
                     QKTESMKEYVDYLCANRAELDALFQDILIHVTSFFREPEAFKILQTRTFPRIMSSKKQ
                     GEGIRIWVPGCSTGEEVYSLAIALLEFLGDRASSTPVQIYGTEISEQAIEKARSGTYS
                     EASTRDVSPLRLRRFFTKVDHGYRVNQTIREMCVFARQDLTKDPPFSRMDLISCRNVL
                     IYLGAALQKRIVASFHYGLLDTGFLLLGKSESLAANADLFTLAEKKGRVFAKKANGGM
                     VAPDVLSIREKTNTGAGPISEKPPAYDVLKEADRLVWQRYSHAGLVLNDDLEILHFRG
                     ETSNYLSPTSGKASLHVLRMIREELKMELRSVIHRARRQNVPVRGTSVRMRRNGGTQE
                     VSLEVVPLVRPLDKERHFLVLFDRPGKTAAPPETKEPAGTRRGADDVREIGHLRRELD
                     ASREYLQSIIEQQEASNEELTSANEEILSSNEELQSSNEELETAKEELQSSNEELVTV
                     NETLQVRNAELAQLSDDLNNVLSGTNIPILIVGEDRRIRRFTPAAERLLNLLPSDVGR
                     PLGNIRPNIDSEDLDALITEVSRTLADLQREVRDKEGRWYSMRIRPYKTSENRIDGVL
                     VALFDIDDLKRSDDALQETKATVSALLGTLVRGILAVDANGRIALVNPGAGQMFGYRP
                     EDMLGQPVEMLLPEQSHAAHAEHRARYFDAPTIRAMKPDRQPVGRRKDGTEFPVEVSL
                     SYLQTAKKWLAVAFVADITELKRAEAALAEKNAALLLSDQGLRTLTATLLTAQEEERE
                     RLGRELHDDLSQHLAALAMEAGAMVPQIPGSVAELRHKVQVLYGSLGRLSEDVRRIAH
                     ELHPSILEHFGLAVALRSYCEEFSKRNDIKIRFRRRDVPESIPGDAALCLYRVAQECC
                     RNIAKHSGARTASVVLAGAAGGIHLAVRDRGVGFDPDQATKGLGLIGVKERVRLAGGS
                     LSVWSRPGQGVGIEVRIPLEQRAT"
     gene            3804..>4099
                     /locus_tag="VLE22_01410"
     CDS             3804..>4099
                     /locus_tag="VLE22_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB13083.1"
                     /translation="MKRTRVLLADDHTLVLEGLKKLLEAEVDLVGTVTDGRALIDAAQ
                     QLRPDVILLDISMPLLNGVEAARRLRKTVPGAKLIFVTMHADPTYVAEALRVGAS"
BASE COUNT          858 a         1171 c         1307 g          763 t
ORIGIN      
        1 gggtgggaca acctgcgcgt gtaccacgtc ctgaagtagg gagtcgtgcg acgtccctgc
       61 aggggcttca ggttgtcagc ggtgcataat gatcttgacg gcagaggagt tgcccatgcg
      121 aagaactccc ccatcgaact cgaaaaagaa acggcacgat gtgtctagcg caaacgccgt
      181 tcggccgacg acttacgaga ccgccgagag ggaagcggcc tccttcccca tcgtcggcat
      241 cggcgcctcc gccgggggct tcgaggccct aatgcagatg ctgcaagatc tgcccccgga
      301 cactgggatg gctttcgtgt tcgttcagca catggaccct gaacacaaaa gcatgctgcc
      361 gaaattgctc tcaaaggcca cccagatgcc cgtggtcgag gcgcagcagg gcatggtcgt
      421 gcaacccaac catgtctatg taattccgcc gaagtccgac ctgggtgtca tcaatggagt
      481 cctccagatc ctgagtcgca ggaagactgg ggggcgctac ctgcccgtgg attacttcct
      541 gtgctcgctg gcggaggatc aaggaggccg cgccatcgga gtgattctgt ccggcactgc
      601 ctcggatggg accgtcgggc tgaacgccat caaggccgag ggtggcatca ctttcgcgca
      661 ggatgacgaa tcctcgaagt actacgggat gccaggcagc gccattgctt cggggtgtgt
      721 cgattttgtc ttgcccccaa atcgcatcgg taaggagctg gcacggctag cgaggcaccc
      781 ttatatcggt ctgccacacc cagccaaaat gccggaactg cttcccgctg gcgaagacga
      841 tttccggaga atcctacatc tgctccgtac cgcttgcggc gtggacttca gcgattacaa
      901 acccggcacc atccgccggc gcatagcccg gcgcatggct ctccagaaaa ccgagagcat
      961 gaaagagtat gtggactatc tttgcgccaa ccgcgccgag ttggacgctt tgtttcagga
     1021 catcctcatc cacgtgacca gtttcttccg cgaaccggaa gcgtttaaga tactgcaaac
     1081 gcgcacattt ccgcgaatca tgtcgagcaa gaagcagggc gaggggattc gcatctgggt
     1141 gcctggatgt tccacgggag aagaggtcta ttctctggcg atcgctctac tggagttttt
     1201 gggagatcgg gcgtcttcga cgccggttca gatttacggc accgaaatca gcgaacaggc
     1261 tatcgagaag gcgcggtccg gcacgtacag cgaagccagc acgcgggacg tctcccccct
     1321 ccggctccgg cgcttcttta cgaaggtgga ccatggatac agggttaacc agacaatccg
     1381 cgaaatgtgc gtcttcgcga ggcaagacct cacaaaagac cctccgtttt cccgcatgga
     1441 cctgatcagt tgccgcaatg tgcttatcta ccttggggcg gcccttcaaa agcggatcgt
     1501 cgccagcttc cactacgggc ttctggacac cggctttctt ctcctcggca agtcggagtc
     1561 gcttgccgcc aatgcagatc tgttcacgct ggccgagaag aagggcagag tcttcgccaa
     1621 gaaggccaat gggggcatgg ttgcgccgga tgttttgtcc atcagagaga agacgaacac
     1681 aggggctggg ccgatttcgg agaagcctcc tgcgtacgac gtactcaaag aagcggaccg
     1741 cctggtctgg cagcgatact ctcatgccgg cctggtactg aacgacgatc tcgagatcct
     1801 ccactttcga ggagagacga gcaattacct ctcgcccacc tcgggcaaag caagcctcca
     1861 tgtgctccgc atgatccggg aggaactcaa gatggagctg cggtcggtga tccacagggc
     1921 ccgaaggcag aacgtgccag tgcggggtac aagcgttcgg atgaggcgga acgggggcac
     1981 ccaggaggtc agtctggagg tggtgccgct agtccggccc ctagacaaag agcgtcactt
     2041 cctggtgctg ttcgaccggc cgggaaagac tgctgcccct ccggagacga aggaacccgc
     2101 cggcacccgg cgcggtgccg atgatgttcg tgagatcggg catctgcggc gggaattgga
     2161 tgcgtcgcgc gagtacttgc agagtattat cgagcagcag gaggcgagca acgaggagct
     2221 tacgtccgcc aacgaggaga ttctctcgag caatgaggaa ctgcagagta gcaacgagga
     2281 gttggagacc gccaaggaag agctgcaatc cagcaacgaa gagctggtga cggtgaacga
     2341 gacgcttcag gtccggaatg cggaactggc gcagctcagt gacgacctca acaacgtgct
     2401 cagcgggacg aatattccaa tcttaatcgt aggcgaagac cgccgtattc gtcgcttcac
     2461 gccggccgcg gaaaggctgc tgaatctgtt gcccagcgac gttggccgtc cgctcgggaa
     2521 cattcgcccg aatatcgact ccgaagacct cgacgctctc atcacagagg ttagccgcac
     2581 gctcgcggat cttcagcggg aggtccgcga caaggagggc cgatggtatt cgatgcggat
     2641 ccggccttac aaaacgtcgg agaacaggat cgacggcgtt ctggtggcct tattcgacat
     2701 tgacgacctc aaacggagcg acgacgcgtt gcaggagact aaagccacgg tgagcgcact
     2761 gctgggaacg ctggtccggg ggatccttgc cgtcgacgcc aacggccgca tcgcattggt
     2821 caaccccggc gcggggcaga tgttcggcta tcgtccggaa gacatgctgg gacagccggt
     2881 ggagatgttg ctacccgagc agtctcacgc tgcgcacgcg gaacatcgcg caagatactt
     2941 cgacgcacca acgattcgag ctatgaagcc tgaccgccaa ccggttggcc gccggaagga
     3001 tggcacggag tttcctgttg aggtgagcct cagctacctg cagacagcga agaagtggct
     3061 tgctgtcgcc tttgtcgcgg acatcaccga gttgaagcgg gccgaagcgg cgctggcgga
     3121 aaagaatgct gctctgctgc tcagtgatca agggcttcga accctgacgg ccactctgct
     3181 cacggcgcag gaagaagagc gggaacgact gggccgggag cttcacgacg acttgagcca
     3241 acatctggcc gcgctggcta tggaggcggg agcgatggtg ccacagatcc cgggctcggt
     3301 ggccgaactg aggcacaaag tgcaggtgct ttacggctcc ttgggcaggt tgtcggaaga
     3361 tgtgcgtcgc atcgcccatg agttgcaccc ttcgatcctg gaacatttcg ggttggctgt
     3421 ggcgctgcgt tcctattgcg aggagttctc gaagcggaac gacatcaaaa tccggttccg
     3481 gcggcgtgac gtgccggaat caatcccagg ggatgcggct ttgtgcctgt acagggtcgc
     3541 ccaggagtgt tgtcgcaaca ttgccaagca ttccggggct agaactgcgt cggtggtact
     3601 ggcgggcgcg gcgggcggca tccacctcgc ggtacgcgat cgaggcgtag ggttcgatcc
     3661 cgatcaggcg acgaagggac tggggctgat cggcgttaag gagcgggtgc gcctcgccgg
     3721 tggatcgctg tctgtgtgga gccggcctgg gcaaggtgta ggaatcgaag tgcggattcc
     3781 gctcgaacag agagcgacct gacatgaaaa ggacgcgcgt tctgctggca gacgaccata
     3841 ctctcgtcct ggaagggctc aagaaattgc tcgaggccga ggtggacctc gtaggtacgg
     3901 tcaccgacgg ccgggcgctg attgacgccg cccagcagtt gaggccggat gtcattctcc
     3961 tggacatctc gatgccgttg ctgaacggtg tggaagccgc acgacggctt cggaaaaccg
     4021 tgcccggcgc gaagctgatt ttcgtcacca tgcacgctga tcccacctac gtcgccgagg
     4081 cactccgcgt gggggcatc
//
LOCUS       DATIFQ010000036        64888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_967983, whole genome shotgun sequence.
ACCESSION   DATIFQ010000036 DATIFQ010000000
VERSION     DATIFQ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 64888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64888
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967983"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VLE22_01415"
     CDS             complement(<1..647)
                     /locus_tag="VLE22_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSB13084.1"
                     /translation="MKTRLWLIFAIVTTLFWGVWGAFMEFPEKAGFPSTLGYCVWALT
                     MIPCALVALAIIRWRLEYDLRSVFLGSMVGFLGAGGQLLLFEALRTGPAYLVFPIISV
                     YPVVTIILSVTLLKERAGRRAWVGIVFALVAIVMLSYQPPNDANSSGTLWFILAILIF
                     FMWGIQAWVMKFSNETMKAESIFFYMMATGLALAPIALWMTDFSKPVNWGFKGPYL"
     gene            complement(658..2394)
                     /locus_tag="VLE22_01420"
     CDS             complement(658..2394)
                     /locus_tag="VLE22_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HSB13085.1"
                     /translation="MTPKALLSCVLLMFIQGCSRRVGSPVEAWVADPHRHIFHGSFGR
                     DLKQRQIKLEAARNEYVVLQFAAKSASDLRDLSAEASDLKGPSSTIPKSSIRVRYPGF
                     LPVDENGQYTPDPLFEKPSVDLKANQAQPVWIDFQVPADAAPGAYEGAVSLRHSGGSV
                     ADFTIRLDVLDFSLPPVNQDHYYFNILMDPGSVARLHKVERWSEAHWTLLEKYVKNWA
                     AHSQDAITVFFIEDPWEMDTGFPVASVLDWKLPGVWEDIKDPKFEFDYTHFDRFVRMC
                     LDAGIDQNLQAWSPVNMPHHDYAVIWYQDTAAKQTRKLKVTAGSPDYERVWSQFARSF
                     AEHLKQKGWLDITTVGLDEISTENLDKIVPAFRKVAPDLKLMVSGGDEKGKYSDFSPE
                     MAFHFGFVKSEVPFPDTVARRKQGKRTLMYTANTPLRPNTFLFSDPLESRMIPWLVWK
                     YDFDGYIRWAWNFWFDGFMEQPFYKWRSGDMFFVYPGVDGPLDSIRSQMLRKGAQDYE
                     VLWMIRDRLGRLTDKAKAQEARKRIAEAMELATQEADPVRPHRPLPSELPAARKMLNE
                     VLLDLMKATSER"
     gene            2464..3219
                     /locus_tag="VLE22_01425"
     CDS             2464..3219
                     /locus_tag="VLE22_01425"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSB13086.1"
                     /translation="MNHPLLKDKVAVVTGAAAGIGEAIARLFAEEGARVYALDIHTEA
                     GEAVAVSIRAAGGWCAFFAADARRKETVAPAVDDAISRFGRIDVLINNAGVYPRRPFL
                     EMTDEEWDEMLDINLKSVYVCSKLVVPHMVEQRGGKIVNISSVTFFKGLPNLSHYIAS
                     KGGMIGFTRSLAREMAPHGVYVNCIAPGGVQTENEGRVATQEEIARIVAQQALPRRLK
                     PVDVARACLFLSSEWSDGIIGQTINVDGGAVMK"
     gene            complement(3295..3717)
                     /locus_tag="VLE22_01430"
     CDS             complement(3295..3717)
                     /locus_tag="VLE22_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13087.1"
                     /translation="MKKHLLAVTFLVLGLAVLVPSVQAQSSLNLNTKVPFDFTIGSRV
                     MPAGDYEVRGDTVRGALIFQRADRAVSHAVISIPVQAMQPPAKGKLVFTCYDGRCFLS
                     QLWSPLSATGRQFLKSKLEMEVARNSAGRNVLVAATPR"
     gene            3964..5313
                     /locus_tag="VLE22_01435"
     CDS             3964..5313
                     /locus_tag="VLE22_01435"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSB13088.1"
                     /translation="MNPRLVAVSGALAGEVLALANDDPVSLGRDASNRIPISDVSVSR
                     RHCVIAAEGGQYVIRDLDSRNGTFVNELPVRERVLEDGDQIRVGESVFLFLAPATEAG
                     TMLPAEPADEALLTQTVVRLRRGDIEHNMIGESPAMREVYVFIAKVAPKETTVLIRGE
                     SGTGKELVARAIHRNSTRVNGPFVAINCAALTETLLESELFGHEKGAFTGASAQKRGK
                     IEVAAGGTLFLDEVGELPPGMQVKLLRVLQEREFERVGGTRPVRVDIRLIAATNRDLD
                     AAIKAGEFRPDLYFRLNVVSITMPGLRERREDIPLLAGHFAARSSEKAKRRVLGLSAD
                     ARALLVRYDWPGNIRELENAIERAVVLGSSDHILPEDLPEALLEAGRPGEAGASGYHA
                     AVLDAKRRLILDAVEQAGGNLAEAARILELHPNYLHRLITNMNLRAELKRGAGRGSP"
     gene            complement(5321..6100)
                     /locus_tag="VLE22_01440"
     CDS             complement(5321..6100)
                     /locus_tag="VLE22_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSB13089.1"
                     /translation="MTRYVRFSLDRTSPPATREALFGILDGDVVYETLSLGSEQRGAG
                     RPLSGIRLLPPSMPSKIVCVGRNYQEHAKELGNPVPAEPIIFLKPPSSLIASGDAIVF
                     PSISEHLSYEGELGLVIGKRARNVKASAAEQHILGYTCINDVTARDLQKKDGQWTRGK
                     GFDTFCPAGPWIVPRGEVAFDQLRVRTYVDGALKQDGSVGDMIFPVPFIIEYVTRFLT
                     LEPGDLVATGTPPGVGSLKAGSIVRVEIEGVGAIENPVVTE"
     gene            complement(6113..6961)
                     /locus_tag="VLE22_01445"
     CDS             complement(6113..6961)
                     /locus_tag="VLE22_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13090.1"
                     /translation="MSFTVPSLTTEGLALKLGQIELRAGSSQGQPFDWDAWLAQAHQL
                     DTAGLNPGPAQVETSAREWAGKEVFLRVRVFSRQGRDSGWSDFAILRVLPPLNRPSRP
                     VAEAVREGVRLTWQVPGAPAGVAYRIYRRSAAQESASVIGTSGAPEFLDTSAEFGKAY
                     EYTVQAAVKSGQSEAESEISESVSFTPVDRFPPAVPAGLSVLAGTETVELVWERNQET
                     DLRGYRIYRGAGDGPMQSTSELIDVPSYSDGKIESGKRYRYAVTAVDQLGNESAPSNT
                     VEITVP"
     gene            complement(7123..8481)
                     /gene="hslU"
                     /locus_tag="VLE22_01450"
     CDS             complement(7123..8481)
                     /gene="hslU"
                     /locus_tag="VLE22_01450"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: protein motif:HMM:NF003544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HSB13091.1"
                     /translation="MVIYLPAQSTEEGPILDELTPREIVRELDKYVIGQADAKRAVAI
                     ALRNRIRRQKLEPEMAEDVIPKNILMIGPTGVGKTEIARRLAKLASSPFLKVEASKFT
                     EVGYVGRDVESMIRDLVDIAIDMVREDRLEEVADRAEKNAEERLLELLMPAQPESSES
                     IDRTREKLREKLRAGKLDDRTVEIDVKERGPTFEIATNMGVEEMDINLKDVLPGLFAG
                     RSRKRKMRVGEALDYLIQEEEQKLIDMDQVTRLALERVERSGIIFLDEMDKIAGRESG
                     HGPDVSREGVQRDILPIVEGTTVNTRHGFVRTDHILFIAAGAFHVSKPSDMIPELQGR
                     FPIRVELKSLSEADFIRILKEPKNALVKQYQALLDTEGIKLTFTADALEEVARFAARV
                     NESAENIGARRLHTIMEKLLEEVSFEGPDLRKKTVKIDAVYVRKHLADIVKDQDLSRY
                     IL"
     gene            complement(8484..9023)
                     /gene="hslV"
                     /locus_tag="VLE22_01455"
     CDS             complement(8484..9023)
                     /gene="hslV"
                     /locus_tag="VLE22_01455"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: protein motif:HMM:NF003964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HSB13092.1"
                     /translation="MSQRVRSTTVLCVRRDGKVVMAADGQVTFGQEVLKATARKLRRL
                     YNDKILAGFAGSTADAFALFSRFESKLEQHSGNLARSVVELAKDWRTDRALRHLEALL
                     VVADKDNTYILSGSGDVIEPDETVAAIGSGGGFAKAAAMALMENSELSARALAEKAMA
                     IAGQICIYTNESVTYEELG"
     gene            complement(9037..10335)
                     /locus_tag="VLE22_01460"
     CDS             complement(9037..10335)
                     /locus_tag="VLE22_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13093.1"
                     /translation="MNPLSSKPASGPGYQRPAAPVTMFGFIFLFAAIPMKEFGPTTAL
                     WATPAILVAAMLIAWAAESAQFFVAQGFALAILAWLQTLPEFAVEFVYAWRQQVPYLL
                     ANLTGALRLLTGLGWPMIYFVAALFYRRRYRKPMRQIVLEDEHCVEVLGLLVPLVYVF
                     FVWLKGSLGLIDAGVLILMYVAYLAVLRKMPPQEAEGVDELEVIPRTIVKSQRSIRNT
                     LIIGFFVAGGLMIYYAAEPFAGSLLTISVAIGVSPFVFVQWVAPLVSEFPEKVSAFYW
                     ARTVHRAPMALMNMVSSNINQWTLLAAMLPIVYSISRGTASTIPFDDQQKLELLMTIG
                     QSLVGMLFLVNMRLTWWEASTLFSLWFIQFVFSPVAPGPTVLGYIATHIHWWVTVVYL
                     LWFGFGMLRYLFGKRHAEAFRLFAVMWRRHVRPVASAAVK"
     gene            complement(10316..11845)
                     /gene="nadB"
                     /locus_tag="VLE22_01465"
     CDS             complement(10316..11845)
                     /gene="nadB"
                     /locus_tag="VLE22_01465"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HSB13094.1"
                     /translation="MTPDFLVVGAGVAGLRAAIELAEAGSVLVVAKDSLRESSSEYAQ
                     GGIAVALSDDDEVQLHEQDTIAAGDGLCDPEAVRVLVEEGPAAIQQLIDWGAEFDREG
                     LKLAFSREGAHSRNRILHAHGDSTGREISRTLYHKAASLKNVQFHSYMAVTDLLVADG
                     ETVGVVACDSSTRRSVPLRAHAVLLATGGLGRVYTETTNPDVATGDGVATAYRAGAAI
                     SDIEFVQFHPTALHVEGAPRFLLSEALRGEGARLRNAAGEFFMECCHPLKDLAPRDVV
                     ARSIVAEIQRTGERHVFLDLTHLPADFTRKRFPRIYETCLQHGVDLTKDPAPVHPAAH
                     YAMGGVRTDLYGRTNLPRLFAAGEVACTGVHGANRLASNSLLEGVVFGARAGAMMREW
                     AGFSASGSAPLPECVFPETSEENLRRLAWDRCGILRSGEGLAETRERLVHMAQVRCPQ
                     PSRALFELRSLHQVLSLIARCALARQESRGAHYRIDYPERRPEFRKHSLITKTNEPTF
                     F"
     gene            11889..12962
                     /gene="aroB"
                     /locus_tag="VLE22_01470"
     CDS             11889..12962
                     /gene="aroB"
                     /locus_tag="VLE22_01470"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HSB13095.1"
                     /translation="MPTYSVTTPQRTYSAVVERGLLPRAGSYVPSKTGKLFIVTTADV
                     WERHGPVLASGLEGRAHEVLFFPGGEERKRLAEVEMLAEEMVRLGGDRSSLVIAFGGG
                     IVNDLGGFLAAIFMRGIPVLQIPTTLLSQVDAGVGGKTGVNLVTGKNLVGSFHQPLAA
                     LIDPDVLATLPEREYRAGLYEVIKCGIIRSADLFTILAERHADVLSRDPDVVEEIISE
                     SVRIKAEVVSSDEREGGLRRILNFGHTFGHALEAETGYARFLHGEAVAFGMRAAVRLA
                     EMRGTLTAREGAKMADTISLYGPIPPVDGISAKNLVARMAADKKTLQGRVHFVLPVRI
                     GEVEVVSEIDEDDVLAAARATLA"
     gene            12959..13717
                     /gene="ubiE"
                     /locus_tag="VLE22_01475"
     CDS             12959..13717
                     /gene="ubiE"
                     /locus_tag="VLE22_01475"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HSB13096.1"
                     /translation="MSRAKQTESGTKPPGARTEAEASRWVQSMFGRVAHRYDLLNHLL
                     SFNVDRYWRRRTVQRVRDVLRQPGARVMDMCCGTGDLLLALEHERGGPVWGSDFCHPM
                     LVVARRKFAERRSASTVFEADALQLPLPDRSIDLITVAFGFRNVANYRDGLQEMLRVL
                     RPGGVAAILEFSQPPNRLFAAFYDFYSKRVLPRVGGFISGAGDAYRYLPESVRRFPGA
                     EELADEMRRAGFREATFKRMTGGIVALHIGVAGG"
     gene            complement(13714..14007)
                     /locus_tag="VLE22_01480"
     CDS             complement(13714..14007)
                     /locus_tag="VLE22_01480"
                     /inference="COORDINATES: protein motif:HMM:NF021402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HSB13097.1"
                     /translation="MRLRAAQGKLKVEHSIIDGVRELLERLLAQNPEVRSVIPGVIRP
                     VRDARGKVKVRVTVPTANGWKAIALSAGARQELFVSTSLPREDLERAFDQATD"
     gene            complement(14066..14728)
                     /locus_tag="VLE22_01485"
     CDS             complement(14066..14728)
                     /locus_tag="VLE22_01485"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HSB13098.1"
                     /translation="MNQQLVASYWAAQRKDMVERQLKSRGIRDPRVLQAMLEIPRELF
                     LPDESRYLSYRDEPVSIGHGQTISQPYMTALMAQCLELRGTETVLDVGAGSGYHAAVL
                     GALAATVYAIEIIPALADTARSNLETTGRAGNIVVVCGDGSAGYPQHAPYDAISVAAA
                     APDVPSELLDQLDDPGTLVIPVGSLWDQELRVVTKSGGQVSTVVASFCRFVPLRGAAG
                     WR"
     gene            complement(14725..18720)
                     /locus_tag="VLE22_01490"
     CDS             complement(14725..18720)
                     /locus_tag="VLE22_01490"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HSB13099.1"
                     /translation="MSPLRRRLLLLSLGALALLLVAGVSGVLVLRSGWFREKVRQRII
                     REVERATGGKAEIGRFTLDWRSLEARVDSFVLHGTEPPGESPFLRARSIVVGLKVISL
                     LDRDIDIALLTVEAPEIRIRVYDGGRTNIPKPEVTRGPRNPVAQFLDLAIRRFRVDEG
                     VLQIQDRRLPLDLEGERLRARLFYEAAGPRYTGELSVRQLNLGFGAVGPVVFDLDSKL
                     AVTGNRVEFTSARLIRNESVVRFHGSLDNLESPRVNFDFDSDVRLSDIPGSVPLPVAR
                     RGSAKLKGSGFVAGASEYRITGQLTGSGLTVERAEFRVRGAALESSFDLTPGKIIAQR
                     VKAAALHGSFLGSARLEDGARLRVEGNVKDISLQELGQASGLDAGAWSGLLSGPVHLE
                     GTLTGQRLQRVRAGGDVAVTAQTGALPVEGSVHAAYDQRGGTLELGRSYLTTPSSRVE
                     FDGTIGRNLNVGLDSADLNDFLPVLAAFGWTPPAPTLPVLTAGGRASFRGTVSGRFDE
                     PEIAGRVSLSKFEWDGLRFDRLESELAVSSREFRVSSLNVAQNGAAARGDGMLALKGW
                     RPDPAGALSGSLTAKGVQLSQLLQMAGRKLPAEATLSADVRLAGTYQSPEMTASIVAA
                     PLSIQGQRFDRALAEVRYAGGTLDIPSARIESGKTALHGKGAFTHRPDDWRSGSARFE
                     VNGSDIALALVRNLARVPEDLEGRIGVRLRGEVDIEAAQPKLRVLDGAFTLSDLMLAG
                     NPAGKVTATASTASGRDLSVAIDGQVAGASVNGKSSFRLEGGYPGKGELRFTGLSLSA
                     LRPWWRPEANGRELPFEAHAQGTLTFSGALMDAGTWNGRAELSSIEIMPSGRGANTEL
                     FALRNDGATVLTLDRGTLRIDRARFTGTDTDLEATGSVSLKSKNNALQLRFRGSVNLG
                     ILHNFERNLTASGVSTLDASIRGPVAKPEIYGRLELKDASFYLSGIPNGIDKARGVIF
                     LFRDRATIEKVTAETGGGKLTLEGFVGFVGEMSVRLQATAEKVRVRYPEGFSTYMNAT
                     LNLAGTSRRSLLSGDVTILRLGVTPRSDFASLLTSSSKPMVTPATQNELVRNMQFDVR
                     AQTAPDARFETTLTRDVQAEADLRLRGTPYKPVVLGQISVTQGEINFLGNRYNINRGS
                     ISFLNPARLEPLLNLDLETRVRGIDVTVTFSGPPNKLTVSYRSDPPLQPSEIIALLAV
                     GRAPAGSPTLAARQSEQDQSWQQFGAATLVGQALAAPVAGRLQRFFGVSRIKIDPKLT
                     GIENNPEARLTLEQQVSKDITFTYITNLTQTQEQIVRVEWNVNRQWSVLAVRDANGLF
                     GIDFLYRKQFK"
     gene            complement(18717..19361)
                     /locus_tag="VLE22_01495"
     CDS             complement(18717..19361)
                     /locus_tag="VLE22_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13100.1"
                     /translation="MHRRGAILVLVSLSLLRAEIIDRVAVTVGTDVITASDLLEEIRV
                     TAFLNGDKPDFTADARRKAAGRLVEQALIGREMNAGQSPVPEPSEVSRILEDVKKERF
                     ASEPEYRRVLTEYGLQEEDVVRHLFKQLATLRFIDQRFRPAVQILDADLWEYYRYKLV
                     PQWEDKAGKPPSFEEARSKVEEILRQQRVDQTLDNWIKEVRNRIRIEYHEEAFQ"
     gene            complement(19361..22258)
                     /locus_tag="VLE22_01500"
     CDS             complement(19361..22258)
                     /locus_tag="VLE22_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HSB13101.1"
                     /translation="MVCSLGVALRAAPDQYEAKPISVIAFEPREQPIPSSELAEILPL
                     KMNTPLKMETVRTAIERLYATGRYEDIAVDAELRDGKVVLRFLTKSNWFVGRVSVTGV
                     PSPPSVGQLVNDADLNLGYQFLESQTAAAVVNIQQTLQRNGFFGASVEPRFEHDPRTQ
                     QVHIHFEVTPGRRDRFGVPVIRGNPEHPVERIIKATRWKGWLGWKPVTATRVQRGLER
                     IRQSYEKLDHLMAQVSLDSLDHNANTGRAVPALTVDAGPEVEIRTEGAKVSRGRLKEL
                     VPVYEERSVDRDLLVEGTRDIAEYFQTKGYFDARVSFRSLPANQGRQEIVYDIDRGER
                     QKLVHLEIRGNSFFDTATIRERMYVLPSSLQFRRGRYSESMLEQDLASIADLYRTNGF
                     RDVNVTSEVQDDYRGKRGNVAVFVDVKEGDQWFVADLKLEGVNQEDEETIRSMLQSSP
                     GQAFSEANVAADRDNILAYYYNGGYPKAAFEWSLEPSAKPRQVNLRFVITEGPRLYVR
                     EVLITGLDATRPQLVNRQILLNPGEPVSQAKILDTQRRLYDLGIFAKVDTAIQNPEGD
                     EKYKYILYQLDESRKYSITTGFGAEIARIGGSQTSLESPAGSAGFSPRVSFDFSRLNF
                     LGRAHTVSFRSRVSTIQRRGLVTYVAPQFQGYETLDASLTALYDDSRDIRTFSSRRWE
                     TSAQITQRWTRSRTLFYRYSFRRVSIDENTLKIRPELIPLLSQPVRVGIPSGTYVDDR
                     RDDPTDSHKGTYNSVDFGVAAKPFGSQADFLRMLSRNSTYHPVGRKLVLARSVLFGYL
                     QTLSTDPNLPSNQQIPLPERFFAGGASTHRGFPENQAGPRDPVTGFPLGGSALLVFGT
                     ELRFPLIGENIGGVLFHDAGNVYSRIENLSFRVSQRDESDFDYMVHAIGLGIRFRTPI
                     GPIRVDFAYGPNSPRFIGCKGTRDELLFGTCEAVPQRINRFQFHFSLGQTF"
     gene            22339..23361
                     /locus_tag="VLE22_01505"
     CDS             22339..23361
                     /locus_tag="VLE22_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HSB13102.1"
                     /translation="MTARERERYSRQILFTGLGETGQERLLRSRVAIVGCGALGSFQA
                     DALARAGVGRLRIIDRDYVELSNLQRQWLFEEADAEEGLPKAVAAARRVAKINSGVEA
                     APAVDDLTPANAEELLGGVDLILDGTDNFETRYLINDFAVHREIPWIYGAAVGSYGLT
                     MAVLPGRTACLKCIYPEPPGGTQPTCETAGVLNAVTSTIAALQTSEAIKILSGNLEAL
                     SKRLTTVDVWSGVVRQIEQPAPDPSCMACGKRRFVHLEGSGRAPISLCGRNAVQIHER
                     SRPVDLTELKRRLAPLGEVRANEFALRFFLLPYEITVFPDGRAIIKGTTDTGLARSLY
                     ARYIGA"
     gene            complement(23370..24491)
                     /locus_tag="VLE22_01510"
     CDS             complement(23370..24491)
                     /locus_tag="VLE22_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13103.1"
                     /translation="MSFAQLIALLIPAALCAQVAEFRVRETAGLRRFGYPVRARFHCE
                     KPAAALRLLDDGKTVPAQFTPIGDGALEIDFSVSLGPFEVRRYRVEEGPGPAPGAGID
                     VVEIGEAFQVRYPAGLVFEIPKHVRGFLSLVRWSELTYVGSGSRGFVLRSKDGAESEV
                     GSSRPPLDSRILKRGPLSACLRFEGHESLGQSGTVKTTVEMEFPRSKSWVEVRWTVED
                     PDALVAAMEADVGLLLDGDPVLVDFGAGTMVYAALRNDSAALFQADLPSTGPPSWRID
                     VAGGAYAAGQGNVEGWAHVMDRRRATAVAVADFARSRPVTSDAIFVNSRGRLAIRRDF
                     ASTGARSLAFWLHFVDMPVHVGAATSPQSMQTPPRVEWE"
     gene            complement(24488..25777)
                     /locus_tag="VLE22_01515"
     CDS             complement(24488..25777)
                     /locus_tag="VLE22_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSB13104.1"
                     /translation="MLKKLVVVLSLAVLAVAQTQKVPPERLKAREWFQNAKFGMFIHW
                     GVYSVLGDGEWVMNNKKIPISEYEKLAPKFNPTEYHPAQWVSMAKNAGMKYITITSKH
                     HDGFAMWGTKQSTWNIVDATPYTKDVLKMLADECKKQGIKLFFYHSHLDWHHPDYYPR
                     GRTGQSSGRPENGDFNRYLDYMDAQLRELLTDYGEIGGIWFDGWWDRPDAEWRLNKTY
                     SLIHRLQPATLIGNNHHRMPFEGEDYQMFEKDLPGHNTAGFNADAGIGQLPLETCETI
                     NRSWGYNSTDKQFKNTSDLIRYLVRAAGYNANFLLNVGPMPNGKIQPEFVERLREIGQ
                     WMQKNGESIYGTRGGPVTPRPWGVTTQKQGKVFVHVLDWPDELLALPNIANVKSAKLL
                     ATGAAVEWKEIPGGAILRLPHSARDLLDTIIVLETSR"
     gene            26048..26572
                     /locus_tag="VLE22_01520"
     CDS             26048..26572
                     /locus_tag="VLE22_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13105.1"
                     /translation="MSGETPVDAEPLPALRPPVINLHNGLAVRVGFLAGSIAFVLGLL
                     PAPFIGRVALLLFAGFLSVYFYRRRSGESLSIGNGARMGWISGLFCFVIITVLFTINM
                     VMISIVSQEGGLAAFYRQQLGAMGMPAQSIEQVIEVFGSPLQITGLLFTLFVMFTGLL
                     TAGGALGARLLRRD"
     gene            complement(26569..27474)
                     /locus_tag="VLE22_01525"
     CDS             complement(26569..27474)
                     /locus_tag="VLE22_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSB13106.1"
                     /translation="MRHHPHFKAYTALASVCFFWGTTYLGIRMALESFPPLVLVSTRF
                     LLSGSIMLLAGRILGVPLPRGRELAQTAWNGLLILGIGNACLTFAEVWIPSGLAALIV
                     TTSAFWMVGVEALLPGGERLHFPTTAGLTVGCLGAALLLAPGSGGGLRGPLLWGFLVL
                     QFGCLAWSYGSIRQRRMSKQAHPMIGGAIQQLTAGLAFLPPALLIPSQPVVWKSRGVA
                     AMLYLVVFGSIVGYSSYVYALEKLPVALVSIYTYINPIVAVLLGWLFYRESFGAREAL
                     AMAVIFVGVALVKRYSRPPTALELD"
     gene            27547..28476
                     /locus_tag="VLE22_01530"
     CDS             27547..28476
                     /locus_tag="VLE22_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HSB13107.1"
                     /translation="MILTLTVNPAIDRNFTADRLVFEDRAYILSSSMSAGGRGINASR
                     VIHSFGGETTAIVTSGGKPGTKFEQLLADSGFPVEVVRIKHWIRNNLTVTDKTGLTIK
                     LNELGPTLDSAELGRIERAVSRKLPGAKWLMICGSLPPGVGPEFYSKLIAMAREKNVR
                     TLLDADGEALREGVEAGPTVVTPNQQEAERLLGEALITRKHFLEAADRIRRMGAESVV
                     LSLGNRGAVGARAGSTIEVIAPRIDAVSPIGAGDAMAAAFVWSMWRRDDFTDALRWGV
                     AAGTASTKLPGVTFASLEQTKEIYPQVELRTVA"
     gene            complement(28489..28863)
                     /locus_tag="VLE22_01535"
     CDS             complement(28489..28863)
                     /locus_tag="VLE22_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HSB13108.1"
                     /translation="MKTGIVVFAHGSRVESANEAVRAVAAELAGLGGYELVEAAFLEL
                     AEPDLKGAIERLVGLGAERVVVIPYFLTLGRHAAQDLPALVGEIEAAHPGLSVVVTAP
                     LDGHTAMSRILLDRAREGLRDA"
     gene            complement(28865..29521)
                     /locus_tag="VLE22_01540"
     CDS             complement(28865..29521)
                     /locus_tag="VLE22_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13109.1"
                     /translation="MRNCQICETRRPRRFCPGVRGEICSLCCGTEREVSVDCPLDCEY
                     LQEARLHERPPDIDPETVPNRDIQVSESFLRDHEELLAVSVRALFEATADTAGAVDFD
                     VREALDALVRTHRTLESGLVYETRPSNLVAASIQQAFRQAIDEYRKQHRERVGIETVR
                     DADVLGILVFLQRLEIGHNNGRRKGRAFLDFLRTSLPQGQGLPQPAGRLGGSSPLVTP
                     "
     gene            complement(29589..30719)
                     /gene="dgt"
                     /locus_tag="VLE22_01545"
     CDS             complement(29589..30719)
                     /gene="dgt"
                     /locus_tag="VLE22_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HSB13110.1"
                     /translation="MKLSLRGLRFPFGQTRGRRYPEPPHPYRNEFQRDRDRVVHARAF
                     RRLEDKTQVFSAGLSDHFRNRLTHTIEVSQISRTVATVLDLDEQLTEALALAHDIGHP
                     PFAHAGEEELDRRMRQFGERFDHNLHALRIVESFEQRYARFPGLNLTFEVREGIVKHS
                     RDLDPGENPDLDDYLPGLRPPLEAQLIDLADEIAYNTADLDDGFAAGLFSVESVAASV
                     PSFSVILETVSNQFPGASERVHFLEALRRLIDLLVSGLIEGTFAAASEARLRDVEDVR
                     RFPGRLACFTPEARATDTALKDFLRRNVYASEGILEERERSTAMLSGLFEFLLRHPDR
                     LPSPYREQAGEGPVHRVVCDYIAGMTDGFLKRTFQRTVAEPE"
     gene            complement(30723..31427)
                     /locus_tag="VLE22_01550"
     CDS             complement(30723..31427)
                     /locus_tag="VLE22_01550"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB13111.1"
                     /translation="MDRLTRKELKKDVFAQEVGHTVEYVAEHRRQFIRFGSIALVVLV
                     LVAGYFYYSKRQHALRQRELAAATRIQDAGVGPPTGRESILTFPTVEEKNKAAIKAFT
                     ELASRHSGTEEGAIAQYYLGTVAADQGRLDDAANWLKSAAESGSPEYASLANLSLAQV
                     YHLQGKTAEAEKLLRALMAKPTIFVSKEQAIIMLARLLASSRPDEARKLLEPLRTQPG
                     AVSRVALTILGELPKQ"
     gene            complement(31483..32139)
                     /locus_tag="VLE22_01555"
     CDS             complement(31483..32139)
                     /locus_tag="VLE22_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB13112.1"
                     /translation="MKEKGIARVVVADEWELLCEGIAAICERDSPFRVVAHCSDGLSA
                     LRMLESLRPDIALLQLNLPKLFGLAVIDKAVRANVPSRLILMSEKTDRKTVIAALRHG
                     ANGFLAKSDCAARVLDAFHSVLGGGVYVSPQVTLGEIFIPRKCSGTTAPFETLSLREH
                     QVFSLLVEGMRAKEIAARLDLSPKTVDTFRRSLMRKLDIYDLAGLVKFAVRKNLTSVS
                     "
     gene            complement(32315..32659)
                     /locus_tag="VLE22_01560"
     CDS             complement(32315..32659)
                     /locus_tag="VLE22_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSB13113.1"
                     /translation="MSIQVTTRQVGDVSVIDVTGRITLGEGASTLRESIRDLVAKGNK
                     KILLNLSDVSYIDSSGIGELVSGFTTVTNQGGVLRLLGLTKRVKDLLQITKLYTVFDV
                     YDDETSAIRSFA"
     gene            complement(32700..33140)
                     /locus_tag="VLE22_01565"
     CDS             complement(32700..33140)
                     /locus_tag="VLE22_01565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB13114.1"
                     /translation="MGEILSGENGNVDVFLSSTLDSVDDAENLVLKAAEEVGFGDDDL
                     HKIGMAVRETVVNAVVHGNRYNSRKRVHLQVLKGDDRITITVADEGNGFEPKSIPDPL
                     AEENLLQQSGRGILLIRAFVDDYQMQRLVPAGTEVKLVKYLRHD"
     gene            33316..34182
                     /locus_tag="VLE22_01570"
     CDS             33316..34182
                     /locus_tag="VLE22_01570"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSB13115.1"
                     /translation="MGALLVALVLSLPVTAQDFSGVRVEKISGGYAFTEGPVWSRDGY
                     LLFSDVPNNKIIRFEPGEGTAVFRENSDGANGNTFDARGRLYTCESRTRRVTRTGKDG
                     KIEVLAEKWQGKRLNAPNDIVVRKDGHVYFTDPAFGQQGDTRELDFFGVYHISPKGEM
                     EVVAKPAGRPNGIALSPDGKVLYVANSDDRNLRAYDVDRNGKAANEHVVVSRIDGVPD
                     GIKVDERGNIYVTARGVAVYSPGGKLLTTLEFTETPANCAFGDADFQTLYVTGRTSLY
                     RVRLTVKGAVQY"
     gene            34241..34696
                     /locus_tag="VLE22_01575"
     CDS             34241..34696
                     /locus_tag="VLE22_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSB13116.1"
                     /translation="MAVKGRRYPERPIVGVGAIILRRDRVLLVERAGEPLKGYWSLPG
                     GVLEAGESLHDGVQREVREETGLEVEPLGIFEVFERINRDERGRTEYHYVLVDYMCRV
                     TGGELRPADDASRAEWVRRADLRKFQITEGTEDVLRRAFQQRRRGLLKP"
     gene            34693..37119
                     /locus_tag="VLE22_01580"
     CDS             34693..37119
                     /locus_tag="VLE22_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HSB13117.1"
                     /translation="MKTTSSDLLEFEALRELVGRYVASPLGLALLREAAPHTDRGHLE
                     QALADVAEAIEYVQAASKPHPAVRGAAVRIRFDSLSDPTEALQKLRIEGTVLEAKEIL
                     QLTGLMDLASDVKRLLGSVAERFPRLAGRGREIGEFHSLVRDLVGKVLPDGTLADDAS
                     PALARLRRDIDRQRKLVQESLERFLRIHREENVLQEEFVTIRNDRFVVPVIAGRQRKI
                     EGVIHGASGSGHTMFVEPLETIELNNELVRLSEEELREIHHILREMTASLRASGEAIA
                     ATAEVLGDLEFLFAKARFASDFDCTVPRFSPSEARRLSLREARHPLLADVLRRQKKPI
                     IPVSLELTEDCRTLLISGPNTGGKTVTLKTVGLLALMAQAGFPVPCSEAEFPLFGQVL
                     ADIGDQQSIQESLSTFSAHICRVRDMLDAADSTSLVLLDELGRATDPEEGGALGVAVL
                     ERFGSAGAFTLSSTHLMALKVYGASTPGVVNASMGFDDETLRPTYVLKVGAPGKSAGL
                     EIAKRLGMPDVLIGEAGQRLGSRERDLSSFLSDLHRRLDRLAAQEQELREIKEALIAR
                     QKSLATEWERRESAKLKELERRYELVLEKFDSQAQEAIERIVAEAERRKVASSAMRRV
                     GGLKRGLREELEVTVVSTRNESRQGALETPKAQITEGVRVRLKNIREPARVSRKLAGG
                     LIEVQAGFLKLQVSEDDVLEVLPDAGGGAGLPRQVTYRPAVQAAQAVQEINVIGQRAE
                     EACDAVEKFLDNAVLAEATRVRIVHGHGMGILRRAIAELLAKNPNVEKFHAGDQFEGG
                     AGATIVELKG"
     gene            complement(37108..37590)
                     /gene="bcp"
                     /locus_tag="VLE22_01585"
     CDS             complement(37108..37590)
                     /gene="bcp"
                     /locus_tag="VLE22_01585"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HSB13118.1"
                     /translation="MAKTAPVALKEGDPAPPLVLTTDRGEALNLASLKGKTVVLYFYP
                     RADTPGCTTEACEFRDTSKQFAKHNAVILGISPDTLAAQSKFKTKYDLPFTLLCDVDK
                     TAAQAYGVWKEKNMYGKTVMGIERTTFIIGKDGRIAKIFPKVKAQGHAAQVLEALSAL
                     "
     gene            complement(37604..37840)
                     /locus_tag="VLE22_01590"
     CDS             complement(37604..37840)
                     /locus_tag="VLE22_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13119.1"
                     /translation="MSEKETTTASGSTEPRVNCLCMGAGPKLTTMLQCGSESAREHFR
                     NARVEFLKAIRTVIDERIEWLTREEKKGTTVPVE"
     gene            complement(37837..39030)
                     /locus_tag="VLE22_01595"
     CDS             complement(37837..39030)
                     /locus_tag="VLE22_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HSB13120.1"
                     /translation="MRILLFTGKGGVGKTSLAAATGVRMAQLGLRTLVMSVDPAHSLA
                     DSFDLATGLFQGQTADPFPVNSHLAIQEVNIQKEIRRHWQEISSYVVSVLRTTGISGV
                     EAEELAILPGMEELSAMLYVNQYWREKLYDAVVLDCAPTAESMRFVSMPTTLDWYMKH
                     VFPFQRSLLKAVRPLARHVSPVELPTDSYFANVQELFRKLEGIDELLEDPRLTTVRLA
                     TNPEKMVLRETQRAFVYFSLHGLTVDTVIVNRVLPSEVTDSYFDDWRVSQEENLREIE
                     EYFAPVRVKKVPLFAREVLGRPRLEELASILYPDGENPAAVIRTERPYSFEKYDGRYE
                     IRIHLPFATKGEIGLFKKGDELVVEIGTLRRHIGLPTSMAALTPARARLDNKILTVEM
                     KEVAS"
     gene            complement(39035..39682)
                     /locus_tag="VLE22_01600"
     CDS             complement(39035..39682)
                     /locus_tag="VLE22_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HSB13121.1"
                     /translation="MRPVIILFAKAPVAGQVKTRLAERLGAGEAALLHKAFVADMLER
                     IAVLSRVADIELHTNMNTDAWPGTKVARRLQEKGDLGIRMLHALNTALSEHRPQAVIV
                     GTDAPTLPTSHLQTVLSLPADVALMPTDDGGYCVIACRRTHSDMFRGVRWSGPFTLED
                     TLRASWKCGLTTAVGPAWFDVDTPADLARLATGPEVPRHTAAWIEKNRPLLAGLE"
     gene            39735..40079
                     /locus_tag="VLE22_01605"
     CDS             39735..40079
                     /locus_tag="VLE22_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB13122.1"
                     /translation="MFKIGRFRLFAICVSAALAAGAAIAAPKAGKGDAGKGKAVFEQC
                     AVCHNADSTDKKMGPGLKGLFKRAKLASGKPVNESNIRATIDAGGNGMPPYKEMLSDQ
                     EKDNLIAYLKTL"
     gene            40174..40251
                     /locus_tag="VLE22_01610"
     tRNA            40174..40251
                     /locus_tag="VLE22_01610"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40210..40212,aa:Glu,seq:ttc)
     gene            complement(40305..41009)
                     /locus_tag="VLE22_01615"
     CDS             complement(40305..41009)
                     /locus_tag="VLE22_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB13123.1"
                     /translation="MKRASSVTLAFLLGAGAVRAQPPAVASEFYVSFTTLAAPKEARK
                     AYERASREAGRKTPNLVKMSQELERALSVYPQFAAAWYFLGGTLLERHDIDGARRAFE
                     RAVTADPRFPNPYVSLAVIELKSRHFAEAARLADSALRLDPRLTEAHYYSVLANMALH
                     NPDRAKQSINAILETGEDRRYLEVHAMLAEIHVAAGDRASAAVEYRRLLELGPSSTSS
                     GLARERLAEWQAQGQP"
     gene            41173..43119
                     /locus_tag="VLE22_01620"
     CDS             41173..43119
                     /locus_tag="VLE22_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HSB13124.1"
                     /translation="MPSIEPQRDRRLDSWKEIAAYLGRDVRTVQRWEKEEGLPVHRHT
                     HKKLGTVYGFQSEIDAWWESRRASLKSPQPLPKRSRRTWLAVGAAAAIVLGAGVVLVI
                     RFITPEPAPVQFKAVPFTTYPGDELGATFSPDGNQVAFAWNGQNQDNYDIYLKAFDSE
                     TPYRLTTDPEAEMFPAWSPDGRRIAFVRSAAPWKLPAAIYVIPATGGPQTRVAQLSTV
                     ECFFRNESLSWSPDGKWLAFADGDSAEAQSGIHVLSVETGETRRLTLPPAGWGGDELP
                     AFSPDGRSIAFARFRSLTRSDIYLVSLDGGMPRRLSFANQLIRGLVWTPDGKAIVFSS
                     DGLWKVAVTGGSPERLQGTSDLADAPAISPQGGRLIFSRPVSNENIWRVGVQGSRLVG
                     EPTRLIASTRDDTNPQYSPDGRRIIFDSNRSGNWELWVCNSDGSNPIPVSRAGWPSWS
                     PDGKYIAFDDDVKGSSDIYVVSAEGGTPRAITHRGENAGPSWSADGWIYFQSNRSGSL
                     EIWRTRPDGGEQVQVTRKGGTRPAVAPDGKFVYFAKGVPAEAIWRIPAEGGEETLFLD
                     NADEADMGHWAPVKEGFYFVLRKERRKALAFLDFKTGGIAEVMQIEKPWDHSALSVSP
                     DGRSILYPQFDEIGHDLMLVENFR"
     gene            43200..43925
                     /locus_tag="VLE22_01625"
     CDS             43200..43925
                     /locus_tag="VLE22_01625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB13125.1"
                     /translation="MSKETIQAYDLPARVSSYDADMALMHPNRSRMVDIALEVVPFDA
                     KAAFTVLDLGAGTGFFTQAVLRRFPNCRVIAVDGAASMVDLAKTRLGAEASRVEFRVG
                     DFRELSRLIPNEHGEFIFSSYALHHLTPEEKTGVIRDALSFLKPGGWFLNADVVTAGH
                     ERTEARIQDVRIQGIVQRASGRDERFPDAVSVRTFLDDLEARDHDKPLPVTEELRILK
                     QGGLASATVFWLEYREAVTGGVT"
     gene            43964..44593
                     /gene="udk"
                     /locus_tag="VLE22_01630"
     CDS             43964..44593
                     /gene="udk"
                     /locus_tag="VLE22_01630"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HSB13126.1"
                     /translation="MGSRVRVPHLIGVAGPSCAGKTELARGVADRLHAPIISLDSYYV
                     DLAHLPFEERPRTNFDVPESLDKDLLVNQLRQLSRGEKIHVPVYDFTRHVRASDTRCV
                     RAGEFVIVEGLFALYWEELRALLKTTVFVYAKDDICFARRLERDVRERGRTPESVREQ
                     FDSTVRPMAEKYILPTRLFADLVLSGTDPLEQSVATVLDGVRISDARGG"
     gene            complement(44658..44843)
                     /locus_tag="VLE22_01635"
     CDS             complement(44658..44843)
                     /locus_tag="VLE22_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13127.1"
                     /translation="MRSLCLDFLGQQEGQDLVEYTLLLAFVCLVASAFFLGSHKSIYT
                     IWEVTNNNLSQAKETIS"
     gene            45117..46463
                     /locus_tag="VLE22_01640"
     CDS             45117..46463
                     /locus_tag="VLE22_01640"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HSB13128.1"
                     /translation="MPTRRGFLAGAVAGTVSAASQTGGSAVSLRTHEWFGDKLEEFTF
                     PPGWRVSVQHMKGHATSLLAPAAIRTAVQNPVGTLPLREIAAGKKTVAIAFDDLTRPT
                     PTYEIVPHVIEELRVAGIRDENILFVTAYGAHYQMNGLEVAKKLGEEAVRRHPWINHN
                     IWENLADLGTTKAGNHLLINTYYSKADVKITLSGLKAHGTPGYGGGPKLILPGISGMK
                     TIRYMHTEIKRARRPQTGPDGVPIFRVWENEQRQDMIEAARRIGVDFSVQTVYNQDRR
                     PVDVVAGDIVKAHHKAARYAVNHLATEYAKDADIVVVNAYPKGGQLHEHFGWGSRGLK
                     EGGSIVVINQNPMGEFVWHYLDEWKFNEGKSYFEQRAARKRRFPQARQVLIYSQYLQA
                     RELDHPYFPPDAEGVRSWSDVVDRLKQKHRGDVHVAVYPYAGIQHGIARLDLPEET"
     gene            complement(46520..48490)
                     /locus_tag="VLE22_01645"
     CDS             complement(46520..48490)
                     /locus_tag="VLE22_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSB13129.1"
                     /translation="MQSRIRLLLLFASFLATSLAAAPPEGQNPPAHVIYSDRHDSSAP
                     LHTIRPNPPRAGEILELPRKWLPNRPGVTPPGETDTVVQSAPGTEAAPTTTVTFEGVN
                     NVSGVLPPDTVGDIGPNHYVQAVNLAFAIYNKSGTLLYGPANINTLFSGFGGPCQTRN
                     DGDPIVLYDHLADRWLISQFALPNFPFGPFYQCIAISQTPDPTGAWYRYGFQISSNKM
                     NDYPKFAVWPDGYYMSMNQFNQASLSWGGAGAVVFERDKMLLGQSARAVYFDLYSTDP
                     NLGGMLPSDLDGPAPPTGTPNYFAQIDDNAWGYPADQLEIWKFQTNWTQPASSTFTLA
                     RILPTASFDGNMCNGSRNCIPQPGGTKVDAIADRLLFRLQYRNFGTHQAMVTNHTVDV
                     NGADLAGIRWYELRDTGAGWNIYQQGTYSPDSTHRWMGSIAMDSSGNVALGYSASSTS
                     VYPSIRYTGRLASDQLGQMTLGEGTIVAGSGYQTHSSGRWGDYSAMSVNESDCSFWYT
                     QEYYNGVSSASWRTRVGSFQISPCGSPPPPPPPATPVHSGDLDGASSPLPRNKWQARI
                     TVTVHNGSHGTVSGATVSGTWSGGFSGAATCTTDGNGQCSVTTGGIPAKKTSATFTVN
                     NITGTNLTYQASANHDPETDSDGTAITVAKPL"
     gene            48823..49944
                     /locus_tag="VLE22_01650"
     CDS             48823..49944
                     /locus_tag="VLE22_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13130.1"
                     /translation="MPGGRLKLDPRDLYALGAHRGGIDERWFSSTTNADNGPSTPEDE
                     GLSYIASNGKRALLKEAIETLGNDFLGADVMREQGGWNLLCKFFDNLGPIPHHMHQRD
                     EHARRVGRKGKPEAYYFPPQYNVKENNFPYTFMGLEPGTTKDDVRRCLKRWNEGDNGL
                     LYHSRAYKLKPGSGWQIDAGILHAPGSLVTYEPQVNSDVFGMFQSMVEGRPVPWDLLV
                     KDVPPEHHQDLDYIIDMLDWEANVDPEFAKHRLFHPAPARPKDEMAECGYSEQWIAYS
                     TPYYSAKELTVFPGRSVKIRDAAAFGAIVVAGWGAIGKMEVETPTLIRYGQMTKDEVF
                     VTAAAAWEGVVVTNRSDSENLVMLKHFGPGNPDAAGFIA"
     gene            49941..50780
                     /locus_tag="VLE22_01655"
     CDS             49941..50780
                     /locus_tag="VLE22_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB13131.1"
                     /translation="MKFGANTLIWTAAFDRTYLSLLPRIKERGFDGVEIARFDFHDFP
                     AAEIRRNLEQNGLECTFCSALTGDLSLISEDAAVRKKAADFLQYGIRTAAELGARIFM
                     GPFYSQVGYLAGRRRTADEWTRAVEGLQSLGESLAANGVVITIEPLNRFETYFLNTAA
                     DAAALCDEVDHPNVGIALDTFHGNIEEKDLGSAFRTAGKHLKHVHACENDRGIPGSGH
                     VDWRGVFGALHELRYDGWLVIESFGFTIKEIAAAACIWRDLAPSPEDIAFEGLKFLKN
                     RAA"
     gene            complement(50923..53991)
                     /locus_tag="VLE22_01660"
     CDS             complement(50923..53991)
                     /locus_tag="VLE22_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSB13132.1"
                     /translation="MDGQLPENEEQRLAELQSYVVPDVLSGAVFDRLAGLVARLFGVP
                     FTAISVIDRDSQHIKASHGLGIREIPRASSFCTHTILSDQVMVVPDALGDDRFRTCPL
                     VTGSHKVRFYAGVPLRTPTGANIGTLCVMDTTTRELDPPQIAALRDLAGMAMAEGELR
                     ASVAKTIAAAENPGFGDDRYRELFENASDVVYTHDLHGNFTSANKAAQRITGYSREDA
                     LKMNIFQVLDPESQRLTIEMIQQKLGGAPQTTYEVTIVTKDNRRIILEVSTRLLFRKG
                     IPVGVQGIARDITERKRAEAQLRLLKSVVVNSNDGVMVAEADPDDPLGSRIVYVNDAF
                     SRITGYSDVESAGRTPRILHGPKTDEAVLDQICAAVRRWETARVELIHYRKDGSEFWV
                     DLNIVPIVDERGAFTYWVSVLRETTDRKRAEILERDRNQVLELVASNGPLETVLARLA
                     NLVENQCSDLLCSVLLVRDGRLHPGAAPSLPQPIADAFDGLDVTTALGPCETALLGKP
                     LLIPNLAADPLFRREAALLRNLGLRSCWCSPILSGDGRALGVFMIYLREQHEASPAVI
                     ELVDMAGRLAAVAVEQRQLNDRLAYQAMHDALTGLPNRFLFEDRLHQALDQATRHGWH
                     VAVLFIDLDRFKQINDTLGHAIGDALLQQVSRRFESCLRRTDSLGRMGGDEFQLILTE
                     LRDPSDTTRVAQKLLDALKTPFKVDAFELFVTASIGISVFPRDGRDSVTLLRNADNAM
                     YRAKNQGKNIYQFFTPDLGAAALEQLELENALRRAMANGELQLYYQPQLAIEGRLAAL
                     EALLVWNHPKLGVISPSQFIPVAEESGLIVEIGAWVLEEACRQTVAWRQAGYEPVKVA
                     VNVSPVQFARADFVETVAQVLSHSGLDPSFLELELTESVVMRDFEESSRQMERLRALG
                     ASISIDDFGTGYSSLSYLRRLPIDTIKIDRSFVKELDVDSNTMPLVQAIVSLAHGLGL
                     DVVAEGVESENQMEALRAVGCDKVQGYLLSASLPASQVESLLVRGAGIRS"
     gene            complement(54111..56375)
                     /gene="malQ"
                     /locus_tag="VLE22_01665"
     CDS             complement(54111..56375)
                     /gene="malQ"
                     /locus_tag="VLE22_01665"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HSB13133.1"
                     /translation="MSKPTLSFVPSKSHEEALHRAASLFGIEREYWDVFGNRVVTRPD
                     VIAAILRALGVPCDTQECLDRAMEERLWCSWSRPLPAVLVVSENAPMVELPLAIPDEA
                     AGWPVAVEFRLEDGTHQSLDLRVDGLPSVEAAELRGRRFRKVRALVPLRIPLGYHDVR
                     VQVRGDHGPVCATMRLIVTPDRVWLPSSLASGRRAAGVAVNLYGLRSERNWGCGDFSD
                     LERIVDWAAEDAGVEFVALNPLHAIHNRQPYNTSPYLPVCIYYKNPIYLDVERVEDFR
                     TSAEAVAAFPSPEVQAEICALRTAPFVEYERVYALKLRFLRLAFREFSRSSAAHSVRA
                     EAFRRYCERESDLLDRYATFCALDEWIHARNPDTWVWAEWPSEYQDPESEATRRFAAE
                     NASLVLFHKYLQWLADLQLQEAQEHARRQGLSIGLYHDLALATDRFGSDFWAHRRCYV
                     SGCRVGSPPDAFAPEGQDWAFPPPNAEYHSETGYRFFVESIRRNCSHGGALRIDHVMR
                     FFRLFWIPDGGQAADGTYVLEKHGDLLRILALESVRNQVLVVGEDLGTVAPKIREELG
                     RFGILSYRLLYFEKNEQDEFRTPHEYPRQSLASITTHDLPTLTGFWLGRDIEARRVAG
                     LLGNGDAYQRQLGVRAREKQNLLDVLLRCGDLPEWFPRNCADVPELSGELHNAVVGFL
                     ASTPSMLMLLALEDLTKETDQQNLPGSTWQYPNWRRKVKFTVEEFRSDPQARDFTAML
                     RGWLRKTSRLMRDD"
     gene            56482..57051
                     /locus_tag="VLE22_01670"
     CDS             56482..57051
                     /locus_tag="VLE22_01670"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSB13134.1"
                     /translation="MGLDASARFNMTGVSDVPENFFARVDEESLVALSDGVVTTTLGQ
                     AINWARKNSIWPMTFGLACCAIEMMSMSASRFDIARFGAEVFRGSPRQSDLMIIAGRV
                     SNKMAPVIRQLYQQMPEPKWVISMGACATSTGVFSNYALIPVNQIIPVDVYVPGCPPR
                     PEQLIYGIMLLQKKMQQEAGTLKDVLNLG"
     gene            57165..57629
                     /gene="rimP"
                     /locus_tag="VLE22_01675"
     CDS             57165..57629
                     /gene="rimP"
                     /locus_tag="VLE22_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HSB13135.1"
                     /translation="MHETVIDKVREIARRVGDSEGIEIVDVEMAGAGNGRRLRIFIDK
                     PSGVTHADCESISQQVGAVLDVEDVVPGGRYTLEVSSPGVERKLTGARDFERFQGKKA
                     KIVLREPVENRKVWEGTLAGVADGVVTIEPAPGKQVRIGLQQVQRANLKFEW"
     gene            57665..59233
                     /gene="nusA"
                     /locus_tag="VLE22_01680"
     CDS             57665..59233
                     /gene="nusA"
                     /locus_tag="VLE22_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HSB13136.1"
                     /translation="MGTIYQSIEILSKEKGIDPQIVLDAVKDAMLAAARKHFRTEEEL
                     VADFDQRAGSIQIFAVFKVVENVEDPVKEVTVEQARRIDPAAELGTEVRVSKPTDLLG
                     RISAQTAKQVILQKVREAERETIYQEYSGRVGELVNCTVKRVEGLDLVVDLGKTEARL
                     PKKEQSKLESFSVGERFRCVIRSVEKAGKNAGLIVSRAAPELVMRLFEQEVPEIYDNT
                     VVIKGCAREAGERTKIAVWSRDRDVDCVGACVGMKGMRVQSIIRELRGEKIDIIEYSD
                     DPVVFATHALSPAKISRVTVIEAGDKHMEVIVDDSQLSLAIGKKGQNVRLCAKLLGWR
                     IDIKSEEEKRQEVESQMAALVVPGAPVSVLIDQGLTEKTVEKLLEAGVSTVEKLGSMT
                     PEELEEIPGIGPKMVEKIQVAVNNYYGQYEEAMAGSPEAVAAVTAAIESMPPGEVPEG
                     QAEPAAEAIPAGSEQSDAVATQGDSQMPDAAASEGESTDSRVSAPEGESKDESKPREN
                     GSGDGESDTIKNVE"
     gene            59344..62217
                     /gene="infB"
                     /locus_tag="VLE22_01685"
     CDS             59344..62217
                     /gene="infB"
                     /locus_tag="VLE22_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HSB13137.1"
                     /translation="MSKIRINELARELEVKPNKLLDLLPQFGITEKKTHSSSLEDDIA
                     NKLRRYFGLLVEEPVEAEPEITPREMAPETGPSVEAIEQTTARTDTPTETQPTTVAPV
                     ESLAPTAVPPPDAGPLKESPFAPRTAPQRLRPPLAGSGHSAQPAAVAPDAIPTPPAPP
                     TPVVASGPGRPAVVAAKPLPVAPRPGQILTGPRQPFPVKPEIVKSSVPSAEMPAEPQA
                     PLPQAATVTHTPRSQAKATLAGQPAARPVVPPRPDLVARLAQTQIKAVPGQPQPRPGR
                     PATAPVPGQPIYRGPIRPGQALMTRPSPGAPGRVARGRPLHPTSARLEPPPPVPTEQR
                     RPGDRRARTPAEREREREREERTLRPSRRDVPVVLPSLNRQITVAEGITVKELSEKLG
                     LKANLVIKKLVERKIFATINQTLDLQLAEELVKAFGATSTRLSYEEEAVQEVGQAEAE
                     QDLLKRPPVVTVMGHVDHGKTSLLDAIRLTNVAEREAGGITQHIGAYHIEKNGRKIVF
                     IDTPGHEAFTRMRARGAKVTDIVVLVVAADDGVMPQTLEAIDHARAAGVPIIVAINKI
                     DRPDAQPERIKQQLADRGLLAEEWGGETVVVPVSAKTKQNLDLLLEMILLVADIQDLK
                     ANPARPAVGSVLEAQLDRGRGPVATVLVRNGTLSVGDYFICGSVFGKVRAMFDDRGEP
                     VKSAEPSMPVEVIGLESLPEVGDTFQMVTDTAKAKQIVIYREAKAREQAMARSSRVTL
                     DQLHEQIRAGEVKELPIILKTDVGGTAEVLTDTLQKLSTDKVRVRVLHASVGAITETD
                     VLLASASNAVIIGFNVRPERNAAQLAENEKVDIRLHTIIYDLVDEMKRAMTGMLEPVF
                     REVFRGRAEVKEVFRITKVGIVAGCQVIDGTVTRDSEVRVLRDNVVVHTGRVGSLRRF
                     KNDVSEVKSGMECGVSLENFGDVKPGDVIEAFVTEQVAAEALA"
     gene            62275..62562
                     /locus_tag="VLE22_01690"
     CDS             62275..62562
                     /locus_tag="VLE22_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HSB13138.1"
                     /translation="MATIGVLTLEMRLEHAHSLKDKRHVVQGLKDRLRHRYNVAVAEI
                     DCQDLWQRAVVAAVTVSGEHAHAERVLQSVEREASSLLGAALVGATVEWLE"
     gene            62569..62949
                     /gene="rbfA"
                     /locus_tag="VLE22_01695"
     CDS             62569..62949
                     /gene="rbfA"
                     /locus_tag="VLE22_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HSB13139.1"
                     /translation="MDEHRTQRVSEAIREELAEIIGYEMADPRLGGIDITEVLITPDM
                     RRAQIRVHLGGGEQARQQAMTALDGARGFLRRKLAARLRLFRVPDLRFEADAFVSDSR
                     RLEKLLRRTRRGRPQPEVSDEKEP"
     gene            62952..63917
                     /locus_tag="VLE22_01700"
     CDS             62952..63917
                     /locus_tag="VLE22_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HSB13140.1"
                     /translation="MKHFVVLLLLFPALLCGEELLRLEDTADAMGATYTVVLYGDDQE
                     QLQSASQAAFDEVRRLDRMLSNYRPQSEWSEVNRSAAERPVRVSPELFELLEACLSYS
                     RASEGAFDVSVGPLMRVWGFYKGTGRLPMKEEIAAVLPVVGYRNIELDRENQTVHFRR
                     RGVELDPGGIGKGYAVDRMVARIREFKITSALVSAGGSTIYGLGTPPGDKGWKVKIRH
                     PREWNETIEEAYLKDESMSTSGDYEKFFEAEGKVYSHIMDPRSGYPAPGMLSVSVIAK
                     KSLDSEAWTKPFFILGRGWAAKHKPQGLRAFTCEDRSELACAWLQ"
     gene            63899..>64888
                     /gene="hemE"
                     /locus_tag="VLE22_01705"
     CDS             63899..>64888
                     /gene="hemE"
                     /locus_tag="VLE22_01705"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HSB13141.1"
                     /translation="MRVASMNSRFLDACRRRPTDVRPVWFMRQAGRYMKQYRDIRAKY
                     GILDICKRPDLAATVTLQPVEILDVDAAIIFADLLLPVEPMGMKLRFAAGEGPVIENP
                     VRTSQDVDTLSISNTDELGYVGEAIQTVVKSLGGKVPVVGFVGAPFTLASYAIEGGAS
                     RTFLRTKQMMYSNETLWRRLMGKLVDVLGPFALMQVASGARSIQVFDSWVGALGPDDY
                     VRFVAPYSRALIERIRSSGVPVIHFGTGAAGFFRELHAAGGDVMGVDWRINIDQAWMD
                     ISYRSAIQGNLDPAALFAPLPELKAKVHELLKRTGTRPGHIFNLGHGILPETPV"
BASE COUNT        12969 a        19033 c        20926 g        11960 t
ORIGIN      
        1 aggtacggtc ctttgaaccc ccagttgacc ggctttgaga aatccgtcat ccacagcgcg
       61 atcggagcaa gagccaggcc ggtcgccatc atgtagaaga agatactctc ggccttcatg
      121 gtctcgttgg agaacttcat cacccaggcc tggatgcccc acatgaagaa aatcaggatc
      181 gcgaggatga accagagggt gcccgaggag ttcgcatcgt tgggcggctg gtaggagagc
      241 atcacgatcg cgacaagagc gaagacgatg ccgacccagg cccgccggcc ggcgcgctcc
      301 ttgagcagcg tgaccgagag gatgatcgtg accaccgggt agaccgagat gatcgggaag
      361 acgagatagg ccggtccggt ccgcaacgct tcgaagagca gcaactggcc gccggcgccg
      421 aggaaaccga ccatcgaacc gaggaacacg gagcgcaggt cgtactcaag cctccagcgg
      481 atgatcgcca gggcgacgag cgcgcacggg atcatcgtga gcgcccagac gcagtagccc
      541 aaggtggatg gaaaaccggc cttctcgggg aactccatga aggcgcccca gacgccccag
      601 aacagagttg taacgattgc gaaaatcagc cagagtcttg ttttcacgag tgcgggttca
      661 cctttcggat gtcgctttca tcagatcgag caggacttcg ttcagcatct tgcgggcggc
      721 cgggagttcc gaggggagcg gacgatgcgg gcggacgggg tccgcttcct gggtcgcaag
      781 ctccatcgct tcagcgatcc tctttcgggc ctcctgcgct ttggctttat ccgtgaggcg
      841 gcccaggcgg tcgcggatca tccagaggac ctcgtaatcc tgggcaccct ttcgcagcat
      901 ctgcgagcgg atgctgtcga gcgggccgtc cacgccgggg tagacgaaga acatgtcgcc
      961 ggatcgccac ttgtagaacg gctgctccat gaagccgtcg aaccaaaaat tccaggccca
     1021 gcggatatag ccgtcgaagt cgtacttcca gacaagccag gggatcatgc ggctttcgag
     1081 cggatcagag aagaggaacg tgttgggccg gagaggcgtg ttggcggtgt acatgagcgt
     1141 gcgcttgccc tgcttgcggc gcgcgacggt gtcggggaag gggacttcgg acttgacgaa
     1201 accgaagtgg aaggccatct cgggcgagaa gtcgctgtac ttgcctttct cgtcgccgcc
     1261 gctgaccatc agcttcagat ccggcgcgac tttgcggaac gccggcacga ttttgtcgag
     1321 gttttcggtg ctgatttcgt cgaggccgac cgttgtgata tcgagccagc ctttctgctt
     1381 caggtgctct gcgaagctcc gggcgaactg gctccacacg cgttcgtagt ccggcgaacc
     1441 cgcggtgacc ttgagtttcc gcgtctgctt cgcggcggtg tcctggtacc agatgacggc
     1501 gtaatcgtgg tgcggcatgt tgacgggcga ccacgcctgg aggttctggt cgatgccggc
     1561 atcgaggcac atccggacaa agcggtcgaa gtgggtatag tcgaactcaa acttcggatc
     1621 tttgatgtct tcccagacgc cggggagttt ccagtccagg acgctggcga cggggaagcc
     1681 ggtgtccatc tcccagggat cttcgatgaa gaagacggta atcgcgtcct ggctgtgcgc
     1741 ggcccaattc ttcacgtact tttcgaggag agtccagtgg gcctcagacc agcgttcgac
     1801 tttgtggagc cttgcgaccg agccggggtc catgaggatg ttgaagtagt agtggtcctg
     1861 gttgacaggc ggcaggctga agtcgagcac gtcgaggcgg atggtgaagt ccgcgacgga
     1921 gccaccggag tggcggaggg agacggcgcc ctcgtaagct ccgggggcgg cgtcagcggg
     1981 aacctggaag tcgatccaga cgggctgggc ctggttggct ttgagatcga cggacggctt
     2041 ttcgaagaga gggtcggggg tgtactggcc gttctcgtcg acggggagga agccggggta
     2101 gcggacgcga atcgaggatt ttgggattgt cgatgacgga ccctttaggt cggacgcttc
     2161 ggcggagagg tcgcgtaagt cgctcgcgct cttggcggcg aactgaagga cgacgtattc
     2221 gttccgcgcg gcttccagtt tgatctgacg ttgtttcagg tcccggccga agctgccgtg
     2281 gaagatgtgg cgatgcggat cggcgaccca tgcttcgacc ggcgatccaa ctcttcggga
     2341 acagccttgt atgaacatga gcagcacaca cgacagcaac gcttttggag tcacgatggc
     2401 gccctcgctt ccgcggaaca ctcattgtag ctgtgtcgcg ggaccgttcg ttatagtggc
     2461 agcgtgaacc atccgctact caaggacaag gtcgcggtcg ttaccggagc agccgcgggc
     2521 atcggtgagg cgatcgcgcg gctgtttgca gaggagggag cgcgagtcta cgccctggac
     2581 atccatactg aggcaggcga agcggtggcg gtctctatcc gggcagcggg agggtggtgc
     2641 gccttcttcg ccgccgacgc gcgacggaaa gagacggtcg cgccggcggt ggacgacgcg
     2701 ataagccggt tcgggcgcat cgacgtcctg atcaacaacg cgggcgtcta tccgcggcgg
     2761 ccgtttctcg aaatgacgga cgaggagtgg gacgagatgc tcgatatcaa tctgaagagc
     2821 gtctatgtct gctcgaagct tgtggtcccg cacatggtgg agcagcgagg cgggaagatc
     2881 gtgaacatct cgtcagtcac gttcttcaag ggcctgccga atctgagcca ttacatcgcg
     2941 agcaagggag ggatgatcgg attcacgcgg tcgctggcgc gggagatggc cccgcacgga
     3001 gtctatgtga actgcatcgc gccggggggc gtccagacag agaacgaggg gcgagtggcg
     3061 acacaggagg agatcgcgcg gattgtcgcg cagcaggcgc tgcctcggcg gctgaagccg
     3121 gtggatgtgg cgcgcgcgtg tctatttctg tcgagcgaat ggagcgatgg gatcatcggg
     3181 cagacgatca atgtggatgg cggagcggtg atgaagtagc cgcacacgaa aacaacccgg
     3241 gaccgaaacg gccccgggtt gcctcgctca ccggtgcata gtcatggccg ttcgtcagcg
     3301 gggagttgcc gcgaccagaa cattccgccc ggcgctgtta cgagcgactt ccatctccag
     3361 cttcgacttc aggaactgac gtccggtagc gctcagcggt gaccaaagct gcgacaggaa
     3421 gcagcggccg tcgtagcacg tgaagacgag tttccccttt gcgggaggct gcatagcctg
     3481 taccgggatc gagatcactg cgtggctgac ggcgcgatcc gcacgttgga agatgagcgc
     3541 tccacgcacc gtatcacctc ggacttcgta gtcaccagcc ggcataaccc ggctgccgat
     3601 ggtgaagtcg aacggaactt tcgtattcag gttcagagag gactgcgcct ggacgcttgg
     3661 caccagtacc gcaagcccca gaaccagaaa cgtgacagca agcagatgct ttttcatcgt
     3721 atcctccgtt tccccggcga ccctctgtga tcgccgtgat atccggataa gtgcaggcgt
     3781 cgggccatgg ccggacctgc ccgaggaaac ggccgtatcc gatagcaggc aaaagagggc
     3841 gggccattcg ctgaacacga gcacggtgtt gtgggaggct gcgaaaccta tccgctcgga
     3901 tagaacatct ctccgtttcg ttagagtaaa ggccgtatac tggagcgggg gctggggccg
     3961 cgcatgaatc cgaggctcgt tgcggtaagc ggtgcccttg ccggcgaagt gctggcgctg
     4021 gcaaacgacg atccggtctc tctgggacgg gacgcttcca acaggatccc gatcagcgac
     4081 gtctccgtct cgcgccgcca ttgtgtgatc gcggcagagg gagggcagta tgtcatccgc
     4141 gacctcgata gccggaacgg gacgttcgtg aatgagttgc cggtccgcga gcgtgtactc
     4201 gaagacggcg accagatccg ggtgggcgaa tcggtattcc tctttctggc gccggccacc
     4261 gaggccggca cgatgctacc cgccgagccg gcagacgagg cgctgctgac ccaaaccgtg
     4321 gtccgcctgc gcaggggaga catcgagcac aacatgatcg gcgagagccc ggcaatgcga
     4381 gaggtctacg tgttcatcgc caaggtcgcg ccgaaagaaa caacggtgct gatccggggc
     4441 gagagcggca caggcaaaga actggtggct cgagcgattc atcgaaacag cactcgagtg
     4501 aacggaccat ttgttgcgat caactgcgcg gcgctgacag agacgctgct cgaaagcgaa
     4561 ttgttcgggc acgagaaagg cgctttcacg ggagcgtcgg cgcagaagcg cggcaagatc
     4621 gaggtcgccg ccggcgggac tctctttctg gacgaggtcg gcgagctgcc gccgggaatg
     4681 caggtgaagt tgctgcgggt gctccaggag cgggagttcg agcgggtggg cggcacgcgc
     4741 cccgtgcgtg tggacatccg gttgatcgcc gcaaccaacc gcgatctgga cgcggcgatc
     4801 aaggcgggcg aattccggcc ggatctctat ttccggctta acgtggtgtc catcacgatg
     4861 ccggggctgc gggagcggag agaggacatc ccattgctgg ccgggcattt tgcagcccgt
     4921 tccagcgaga aggcgaagcg ccgggtgctc gggctctccg cggacgcgcg tgcgctcctg
     4981 gtccgctacg actggcccgg aaacatacgc gaactggaga acgcaatcga acgcgccgtt
     5041 gttctcggtt cgagcgatca cattctcccg gaagacctgc cggaggcgct gctggaggcc
     5101 ggacgaccgg gagaagcggg cgcgagcggc tatcacgcag cggtgctcga tgcgaagagg
     5161 cggctgattc tcgacgcagt ggaacaggcg ggcgggaacc tggcggaagc ggcaagaatc
     5221 ctcgaactgc acccgaacta cctgcaccgc ctgatcacga acatgaacct gcgggccgaa
     5281 ctgaagcggg gggcagggcg cgggtcgccg taggccgcag ttactcggtc acaaccgggt
     5341 tttcgatcgc gccgacaccc tcgatctcga ccctgacgat cgaaccggcc ttgagcgaac
     5401 cgacgccggg cggcgtgccg gtggcgacga gatctccggg ctcgagggtc aggaagcggg
     5461 tgacgtactc gatgatgaac ggcacgggga agatcatgtc accgacagaa ccgtcctgtt
     5521 tcaatgcgcc gtcgacgtaa gtgcggacgc gcaattggtc gaacgcaact tcgccacgcg
     5581 ggacgatcca tgggccggcc gggcagaacg tgtcgaagcc tttgcctcgg gtccactggc
     5641 cgtccttctt ctggaggtcg cgggcggtga catcattgat gcaggtgtag ccgaggatgt
     5701 gctgctccgc ggccgacgcc ttcacgttgc gcgcccgctt gccgatgacg aggcccagtt
     5761 cgccctcgta gctcaagtgc tcggagatcg acgggaaaac gatcgcgtcg cccgaggcaa
     5821 ttagcgacga aggcggcttt aggaagataa tcggctcggc gggcaccgga ttgcccagtt
     5881 ccttggcgtg ttcctgatag ttccggccga cacagacgat tttcgatggc atcgacggcg
     5941 gaagcaggcg gatgccggag agtgggcgcc ccgcaccgcg ctgttccgac cccagtgaca
     6001 gagtttcgta cacaacgtca ccatcgagaa tcccaaacag agcctcgcgg gttgctgggg
     6061 gcgaggtgcg gtcgaggctg aaacggacgt atctggtcat gagcgtgtcc tgtcagggta
     6121 ccgtgatctc gacggtgttc gagggagcgc tctcgttgcc aagctggtcg acagcggtga
     6181 ctgcataccg gtagcgtttc cccgattcga ttttgccgtc actgtaactg gggacgtcga
     6241 tcaattcgct ggtgctctgc atcggaccgt cccccgctcc gcggtagatg cggtaaccgc
     6301 gcaggtcggt ctcctgattc ctttcccaca ccagttcgac ggtttccgtg ccggcaagca
     6361 cgctcaggcc ggccggcacg gcgggcggaa aacggtccac cggggtgaat gagactgatt
     6421 cggagatctc gctttccgct tccgattgtc cggacttcac tgctgcctgt acggtgtatt
     6481 cgtatgcctt tccaaactcg gcggaggtat cgaggaattc cggcgcgcca gaggttccga
     6541 tgacagacgc ggattcctgg gcagcggacc ggcggtaaat tctgtaagcg acaccagcgg
     6601 gagcgccggg aacctgccag gtaagacgca caccctcgcg gacggcttcc gccacggggc
     6661 gcgacgggcg gttgagcggg gggagcactc ggagtatcgc gaaatccgac caaccgctgt
     6721 cacggccctg ccggctgaaa acacgcacgc ggagaaagac ttccttgccg gcccactcgc
     6781 gggccgaggt ttcgacctgc gccgggccgg ggttcaagcc cgcggtgtcg agctgatgag
     6841 cctgggccag ccaggcatcc caatcgaacg gctgcccttg cgaggagcct gcccgcaatt
     6901 caatctgacc gagtttcaga gcaaggcctt cggtagtgag gctcggtacg gtgaacgaca
     6961 ctaggatcct gtcgccccgc tgatgcgctt tcaggtcatt gacacgagtg gggatattga
     7021 gcgcgggcgg gagcgggtcg ccgacgtaac cgcaagagta cgcgaccaaa gagagaagag
     7081 gcagggcaag ggagcgccag gttattgccg tccgccgtgg tctcaaagga tgtaccggct
     7141 gaggtcctgg tccttcacga tatccgcgag gtgcttgcga acgtagacgg catcgatctt
     7201 caccgttttc ttgcgaagat cgggaccctc gaaggaaacc tcctcgagca acttttccat
     7261 gatcgtgtgg aggcgccgcg caccgatgtt ctcggcgctt tcgttcacgc gggcggcgaa
     7321 gcgtgcgacc tcttcgagcg cgtcggcggt gaaggtcagc ttgatgccct cagtgtcaag
     7381 cagagcctgg tattgcttga cgagtgcgtt cttaggctcc ttcaagatac ggatgaaatc
     7441 ggcctcggag agtgacttca actcgacgcg gatcgggaag cgcccctgca attcggggat
     7501 catgtcgctc ggtttggaga cgtgaaaggc gccggcggca ataaagagga tgtgatccgt
     7561 gcggacaaag ccatgacggg tgttgacagt ggttccttcg acaatcggca ggatgtcgcg
     7621 ctggacgccc tcgcggctga cgtccggccc gtggccgctc tcgcgtccgg cgatcttgtc
     7681 catctcgtcg agaaaaatga taccgctgcg ctcgacccgt tccagggcga gacgagtgac
     7741 ctgatccata tcgatgagct tctgctcctc ttcctgaatc aggtagtcga gcgcttcgcc
     7801 gacgcgcatt ttacgcttcc tgcttcggcc cgcgaacaat cccggcagga cgtccttgag
     7861 gttgatatcc atttcctcga cgcccatgtt ggtggcgatt tcgaacgtgg ggccgcgctc
     7921 cttgacgtcg atctcgactg tacgatcgtc gagcttgccc gcacgcaact tctcgcgcag
     7981 tttttcgcgg gttcggtcga tgctttcgct cgactccggc tgcgcaggca tgagtagctc
     8041 gagcagccgc tcctcggcgt tcttctcggc acggtcggcg acctcctcga gacggtcttc
     8101 gcggaccatg tcgatggcga tgtcaacaag atcgcggatc atggactcga cgtcgcggcc
     8161 gacataaccc acttcagtga acttgctggc ctcgaccttc aagaacggag aactggccag
     8221 cttcgccagc cggcgcgcga tctccgtttt gcccacgccg gtaggcccaa tcatgaggat
     8281 gtttttcgga atcacatcct cggccatttc cggttcgagc ttctgacggc ggatgcggtt
     8341 gcggagggcg atggcgacgg cccgtttggc atcagcctgt ccgatgacgt atttgtcgag
     8401 ctcgcggacg atttcgcggg gagtcagctc gtcgaggatc ggaccttctt cggtggactg
     8461 agcgggcaga tagatgacca tgactagccc aattcttcgt aggtgacgga ctcgttggta
     8521 tagatgcaga tctggccggc gatcgccatg gccttctccg cgagggcccg cgcactcaac
     8581 tcgctgttct ccatcaacgc catggccgcg gccttggcaa atcccccgcc ggagccaatg
     8641 gcggcgacag tctcatccgg ttcgatgacg tcgccgctgc cgctgaggat gtacgtattg
     8701 tccttgtcgg ctaccacgag cagcgcttcg aggtgtcgca gggcgcggtc ggtgcgccag
     8761 tccttggcga gctccacgac gctgcgggcg aggttgccgc tatgctgctc gagtttggac
     8821 tcgaagcggg aaaacagcgc aaaggcgtcg gcggtggagc cggcgaaacc ggccagaatc
     8881 ttgtcgttgt acagccgccg aagcttgcgg gcggttgctt tgagcacttc ctgaccgaac
     8941 gtcacctggc cgtcggcggc catgacgacc ttgccgtccc ggcgcacaca gaggacggtg
     9001 gtggatcgaa ctcgctggct catgtaaagg agactcctat tttaccgcag cactcgcgac
     9061 cggccggacg tgccggcgcc acatcacagc gaacaagcgg aacgcctcgg catgtctctt
     9121 tccgaagagg taccgaagca tcccgaaacc gaaccaaagc agatagacga ccgtcaccca
     9181 ccagtgaatg tgcgtggcga tgtatcccaa caccgtcgga cccggcgcca ctggcgaaaa
     9241 gacgaactgg ataaaccaga gtgaaaacag cgtcgacgcc tcccaccagg tgagcctcat
     9301 attgaccaga aacagcatgc cgaccagaga ctgtccgatg gtcatcagga gttccagctt
     9361 ctgctgatcg tcgaacggaa tggtggaagc cgttccgcgg ctgatggagt agacgatcgg
     9421 aagcatggca gccagcaagg tccactggtt gatgttgctc gaaaccatgt tcatcagagc
     9481 catcggcgcg cggtggacgg ttcgtgccca atagaaggcg gacaccttct cggggaactc
     9541 cgagacgagc ggcgccaccc actggacgaa cacaaacgga gacactccga ttgcgacaga
     9601 aatggtcagc aggctgccgg cgaaaggctc cgcggcatag tagatcatca aaccgccggc
     9661 cacgaagaag ccgataatca gcgtgttacg aatgctccgc tgcgacttca cgatggttcg
     9721 ggggatcacc tcgagctcgt cgaccccttc ggcttcctgg ggaggcatct tgcggagcac
     9781 ggcgaggtat gcgacataca tcaggatgag aactccggca tcgatcagtc cgagagaacc
     9841 cttcagccag acaaaaaaga cgtaaaccag cggcacgagg aggcccaaca cctcgacaca
     9901 atgctcgtcc tcgaggacga tctgccgcat aggcttacgg taccggcggc ggtagaacag
     9961 ggcggctaca aagtagatca tgggccagcc caggccggtg agaaggcgca gggcgcccgt
    10021 caggttcgcc aataagtagg gaacctgctg gcgccatgcg tagacgaact ccacggcgaa
    10081 ttctgggagg gtctggagcc aagccaggat cgcgagcgca aagccctgag cgacaaagaa
    10141 ctgggctgat tcggcagccc aggcgatgag catcgccgcc acaagaatcg cgggcgtggc
    10201 ccataacgcc gtggttgggc cgaattcctt catggggatt gccgcgaaaa gaaagatgaa
    10261 accgaacatc gttaccgggg cggcaggacg ttgatagccg ggtcccgacg ccggtttaga
    10321 agaaagtggg ttcattcgtc ttggtgatta gcgagtgctt ccgaaattcg ggtcttcttt
    10381 caggatagtc gattcggtag tgggccccgc gactctcctg gcgcgcaagg gcacagcggg
    10441 cgatgagaga gagcacctga tgcaggctgc gcagttcgaa caatgcgcga ctgggctggg
    10501 ggcagcgcac ctgcgccatg tgaacgagcc gctcacgtgt ttcggcaaga ccctcgccgg
    10561 agcgcaggat tccgcaacga tcccaggcaa gccgcctgag gttctcctcg gatgtctcgg
    10621 gaaagacgca ctccggaagc ggagctgacc cggaggcgga aaaaccggcc cactcccgca
    10681 tcatggcgcc ggcccgggcg ccgaacacga cgccctcgag cagggaattg cttgcgaggc
    10741 ggttcgcgcc gtgcacgccg gtgcaggcga cctcgcccgc agcgaataac cggggaaggt
    10801 tggtcctgcc atacaggtcg gtgcgcacgc ctcccatcgc gtagtgggcc gccgggtgca
    10861 caggagcggg atccttcgtt aggtccactc catgctggag acaggtctca tagatgcgcg
    10921 ggaagcgttt ccgggtgaaa tcggccggca ggtgagtgag atcgaggaac acgtgccgct
    10981 ccccggtgcg ctgaatttcc gccacgatgg agcgggccac cacgtcgcgg ggagcaagat
    11041 ccttcagcgg atggcagcac tccataaaaa actcgccggc agcgttacga agccgcgcgc
    11101 cctcgccgcg aagagcctcc gacaacagga agcggggggc tccttcgacg tgcagggcgg
    11161 tggggtggaa ttgcacaaac tcgatatcac tgatggctgc ccccgcccgg taggcggtgg
    11221 ccaccccgtc gccggtagca acgtcagggt tggtcgtctc ggtgtacaca cggccgagac
    11281 cgccggtggc cagcaggaca gcgtgggcgc ggagaggcac cgaacgccgc gtggaggaat
    11341 cacaggctac gactccgacc gtctcaccat cggcgaccag gaggtcggtg acggccatat
    11401 agctgtgaaa ttgtacgttt ttcagagagg cggccttgtg gtagagagtg cgggagatct
    11461 ccctcccggt tgaatcgccg tgggcgtgca ggatccggtt tctactgtgc gcgccctcgc
    11521 ggctgaaggc gagtttcagg ccttcacggt cgaactcggc gccccaatcg atgagttgct
    11581 ggatcgcggc gggaccttcc tcgacgagca cccgcacggc ttcagggtca caaaggccgt
    11641 cgccagcggc aatcgtatct tgttcatgca gttggacttc gtcgtcatca ctgagggcga
    11701 cagcgattcc gccttgggcg tactccgatg aggattcgcg caaactatcc tttgcgacca
    11761 cgagtacgct gccagcctcg gcaagttcta tcgcagcccg aagtccggcg acacccgcgc
    11821 cgacgaccag aaaatcgggg gtcatctaca ctgcgattct agcatcccgc acatggtaca
    11881 accgttttat gcccacgtat tcagtcacga cgccgcaacg aacgtacagc gcggtggtcg
    11941 agcggggctt gctaccacgg gcaggttctt acgtgccgtc gaagaccgga aaactgttta
    12001 ttgtgacgac ggcggacgtg tgggagcggc acggtccggt actggcctcc gggctggagg
    12061 gacgcgcgca cgaggtactg ttcttccccg gaggggagga gcggaaacgg ctggcggaag
    12121 tggagatgct ggccgaggag atggtgcggc tggggggaga cagaagcagc ctcgtcatcg
    12181 cgtttggcgg cggcatcgtc aacgatttgg gaggtttcct ggccgccatc ttcatgcggg
    12241 ggatcccggt tctgcaaatt ccgacgacgc tgctgtcgca ggttgacgcg ggagttggag
    12301 gcaagacggg cgtcaacctc gtgaccggca agaatctcgt gggctcgttc caccaaccgc
    12361 tggccgcgct aatcgatccc gacgttctcg ccacgctgcc ggagcgcgag taccgggcgg
    12421 ggctgtacga agtaatcaag tgcgggatca ttcgcagcgc ggatctgttc accatcctgg
    12481 cagaacggca tgcggacgtg ctgtcacgcg atccggacgt agtcgaagag atcattagcg
    12541 agtcggtgcg aatcaaggcg gaagtagtct cctcggacga acgggagggc ggcttgcggc
    12601 ggatcctgaa ctttggccac acgttcggcc acgcgctcga agcggagacc ggctacgccc
    12661 ggttcctgca tggcgaggcg gtagcattcg gtatgcgagc ggcggtccgg ctggctgaga
    12721 tgaggggcac tctgacagct cgagagggcg cgaaaatggc cgacacgatc tcactttacg
    12781 gaccgattcc accggttgac gggatttcgg caaagaacct agtggcgcga atggccgccg
    12841 acaagaagac actgcaaggc cgggttcact tcgtgctgcc cgtccggatc ggcgaggtgg
    12901 aagtagtgag cgagatcgac gaagatgacg ttctcgcggc tgcccgagcc actctggcat
    12961 gagcagggcc aaacagacag agagcggcac caaaccgccc ggggcgcgga cggaggccga
    13021 ggcgtcgcgg tgggtccagt cgatgttcgg ccgggtagct caccggtacg acctgctcaa
    13081 ccacctgctt tccttcaatg tggaccgcta ctggaggcgc aggaccgtcc agcgggtgcg
    13141 tgacgttttg cgccagcccg gggcccgggt gatggacatg tgttgtggca cgggggacct
    13201 gctgctggcg ttggaacacg agcgaggcgg gcccgtgtgg ggcagcgact tctgccatcc
    13261 gatgctggtg gtggcgaggc gcaagttcgc cgaaaggcga tccgcttcga ccgttttcga
    13321 ggccgacgcc ctccagttgc ctcttccgga ccggtcgatc gacttgatca cagtggcgtt
    13381 cggcttccgg aacgtcgcca actaccgcga cgggctgcag gaaatgctga gggtcctgcg
    13441 cccgggcggc gttgcggcca ttctcgagtt ttcgcagccg ccgaaccgat tgttcgcggc
    13501 attctacgac ttctattcga agagagtgct gccgcgcgta ggaggattca tctcgggcgc
    13561 aggggacgcc taccgttacc ttccggaatc cgtacgaaga ttcccgggcg ccgaggaact
    13621 cgcggacgag atgcgccgag ccggtttccg ggaggcgacc ttcaagcgta tgacgggcgg
    13681 catcgtagcg ctgcacatcg gggtggcggg gggctagtcc gtcgcctggt cgaaggcgcg
    13741 ttcgaggtct tcgcggggga gtgaggtgct gacgaaaagc tcctggcggg cgccagcgct
    13801 caaggcgatc gctttccagc cgttggcggt ggggacggtg acgcggacct tcacttttcc
    13861 gcgggcgtcg cgaacggggc ggatgacgcc ggggatcacg gaacgaacct cggggttctg
    13921 ggccagcaag cgctcgagaa gctcacgcac accgtcgatg atgctgtgct cgactttcag
    13981 cttgccctgc gccgcgcgga gtctcatctg ttaccagttt cgcaccggag gggccgggaa
    14041 ccaaacaaga gacgacgcac gcggtctacc tccagccggc cgcgccccgc aggggaacga
    14101 agcggcagaa cgaagctacg acggtcgaaa cctggccgcc ggacttggtg accacccgca
    14161 actcctggtc ccagagcgaa ccgacgggaa tgacaagtgt tccgggatcg tcgagttggt
    14221 cgaggagttc ggacggcaca tccggcgcgg cggcggcgac ggaaatcgcg tcgtaggggg
    14281 cgtgctgtgg gtatccggcg gagccgtcac cgcaaacgac cacgatgttc ccggcccggc
    14341 ccgtggtttc cagattggaa cgggccgtgt cggcgagcgc cgggatgatc tcgatcgcgt
    14401 acacggtggc ggcaagggcg cccagtacgg cggcatggta ccccgagccg gcgccaacgt
    14461 cgaggaccgt ttccgttccc cggagctcga gacactgagc catgagcgct gtcatgtaag
    14521 gctgcgagat cgtctggccg tggccgattg agaccggctc gtcgcgatag ctgagatagc
    14581 gcgattcgtc gggcagaaac agctccctcg ggatctcgag catcgcctgg agcacgcggg
    14641 gatcgcggat gccgcggctt ttcagttgcc gctccaccat gtccttccgc tgggcggccc
    14701 agtacgaagc taccagttgc tggttcactt gaactgtttt ctgtacagga agtcgatacc
    14761 gaacaggccg ttcgcatcgc ggacggcaag cacggaccac tgccggttga cgttccactc
    14821 cacgcgcacg atctgctcct gcgtctgggt caggttggtg atgtaggtga aggtgatgtc
    14881 tttggatacc tgctgttcaa gcgtgagtct tgcttccggg ttgttctcga ttccggtgag
    14941 tttaggatcg atcttgatac ggctgacacc gaagaagcgc tggaggcggc cggccacagg
    15001 ggcggccagc gcctggccga cgagggtagc agcgccgaat tgttgccagg actgatcctg
    15061 ctcggactgc cgcgcggcaa gggtcgggct gcccgccgga gcgcggccca ccgccaaaag
    15121 ggcgatgatc tcactcggct gcaggggcgg gtccgagcgg taggaaacag tgagtttgtt
    15181 cggggggccg gagaaagtca cggtcacgtc gatgccgcga accctggttt cgaggtcgag
    15241 gttcagcaga ggctcgagcc gggcgggatt caggaatgag atgctcccgc ggttgatgtt
    15301 gtaacgattc ccgaggaaat tgatctcgcc ttgcgtcacc gagatctgac cgaggacgac
    15361 cggcttgtat ggggtgccgc gcaaacggag gtccgcttcg gcctggacgt cgcgcgtcag
    15421 cgtcgtttca aagcgggcat ccggagcggt ctgagcgcgc acatcgaact gcatgttgcg
    15481 cacgagctca ttctgcgttg ccggggtcac catgggtttc gaactgctag taaggaggct
    15541 ggcgaaatcg gaccgcggtg tgacgcccaa gcgcagaatg gtgacgtcgc ccgacagcag
    15601 gcttcgccgg gaggtaccgg cgagattcag cgtggcgttc atgtaggtgc tgaatccttc
    15661 cggataccgc acgcgaacct tttccgcggt cgcctggagc cgaacgctca tttcccccac
    15721 gaagccgacg aatccctcaa gagtcagttt gccgccgcct gtctcggcag tcactttctc
    15781 gatggttgcc cggtcccgga agaggaagat tacacccctg gctttgtcga ttccattagg
    15841 aatgccactg aggtagaacg acgcgtcctt caactcgagc cggccgtaaa tttcgggttt
    15901 ggcaaccggc ccccggatgg atgcgtcgag ggtggagact cccgaggcgg tgagattccg
    15961 ctcaaagttg tgcaggatcc ccaggttgac ggagccccgg aaccgcaact gcagggcgtt
    16021 gttcttggat ttaagggata cggagccggt ggcttcgaga tccgtgtccg ttcccgtgaa
    16081 ccgcgcgcgg tcgatgcgca gagtcccccg gtcgagcgtg aggactgtgg caccgtcgtt
    16141 tcgcagggcg aacagttcag tgtttgcgcc acggcccgat ggcatgattt cgatcgagga
    16201 aagctccgcg cgcccattcc aggtgccggc gtccatcaga gcgcccgaga acgtcaaggt
    16261 tccctgcgcg tgcgcttcaa acggcagttc gcggccgttc gcttccggtc tccaccatgg
    16321 ccgcagcgcg gagagagaga gaccggtgaa ccggagctca ccctttccgg gatatccgcc
    16381 ttcgagccgg aaggaactct ttccgttcac cgaagcgcca gcgacctgac cgtcgattgc
    16441 gacggacaag tcacgcccgg acgccgtcga tgcggtcgca gtgacttttc cggccgggtt
    16501 tcccgccagc atgaggtcgg agagagtgaa ggcgccgtcg agcaccctga gtttcggctg
    16561 ggccgcctca atgtccacct cgccgcggag ccgcacgccg attcgtccct ccagatcctc
    16621 ggggactcgc gccaggttgc gaaccagagc gagggcgata tcgcttccat tgacctcaaa
    16681 ccgggcagac ccgctccgcc agtcatccgg gcggtgcgta aacgctccct tgccgtgaag
    16741 agccgttttg ccggactcga tccgcgcgga cggaatgtcg agggtccccc cggcgtaccg
    16801 cacttcggcg agcgcccggt cgaagcgctg accctggatc gagagaggcg cggcgacgat
    16861 ggatgcggtc atttccgggg actggtatgt gccggcgagc cggacatcag cggaaagcgt
    16921 cgcttcggcc ggcagcttac gcccggccat ctggagcaac tgcgatagct ggacgccttt
    16981 cgcggtcaat gacccgctga gcgccccggc ggggtcgggc ctccagccct tcagggccag
    17041 catgccatcg ccccgcgcgg cagcgccatt ctgcgcgaca ttcagggaac tcacgcggaa
    17101 ttcgcgggag gataccgcga gttccgactc caaccggtcg aaacgcaggc catcccattc
    17161 gaacttcgac agagagaccc ggccagcgat ttccggctca tcaaaccggc cgctaacggt
    17221 tccgcggaag gaagctctgc ccccggccgt caggacagga agagtcgggg caggaggcgt
    17281 ccacccgaac gccgccagca ccggcaaaaa gtcgttgagg tcggcggagt ccaggccgac
    17341 gttgaggttt cgcccgattg tgccgtcgaa ctcgacgcgg gaagacggcg tcgtcaggta
    17401 ggatcggccc aactccagcg ttccgccacg ctgatcgtag gcagcatgga cagagccttc
    17461 aaccggcagc gcgcccgtct gggccgtaac ggcaacgtca cccccagcgc gcactcgttg
    17521 gaggcgctgt cccgtgaggg ttccctccaa gtgaaccggg cccgacagca agccgctcca
    17581 ggcaccggcg tcgagcccgc tggcttgtcc gagttcctga agggaaatat ctttgacgtt
    17641 cccctccacg cggaggcggg ccccgtcctc aaggcgggct gaacccagaa agcttccgtg
    17701 cagggcggcc gccttcacgc gctgcgcgat gattttgccg ggtgtgaggt cgaaagagga
    17761 ctccagcgcg gcgccgcgaa cccggaactc ggccctctca accgtcagac cactccctgt
    17821 aagttgtcca gtgatgcggt actcggaggc gccggcgacg aacccgctgc ccttgagttt
    17881 cgcgctcccg cggcgagcga ccggcagcgg aacggatccc ggaatgtcgg acaagcgaac
    17941 atcgctgtcg aagtcgaagt tgacgcgggg agattccaga ttgtcgagag atccatggaa
    18001 ccgcacaacg gactcgttcc ggatgagccg ggccgaggtg aattccaccc ggttgccagt
    18061 gacggcgagt ttcgaatcca gatcgaaaac gaccggccca acagcgccaa aaccgaggtt
    18121 caactgccgg accgagagct cccctgtgta gcgcgggccg gccgcttcat agaacaggcg
    18181 cgcgcggagg cgctcgccct ccaggtcgag agggagcctg cggtcctgga tctggagcac
    18241 cccctcatcg acgcgaaagc ggcgaatggc cagatcgagg aactgtgcga ccgggtttcg
    18301 gggacccctg gtgacctcgg gcttggggat gttagtgcgt ccgccgtcgt acacccggat
    18361 gcggatctcg ggcgcctcga cagtgagcaa agcgatgtcg atatcacgat caaggaggga
    18421 gatcactttc aggccgacca caatagagcg ggcccggagg aagggagact cgccgggagg
    18481 ctcggtcccg tgcaggacga acgaatcgac ccgcgcctca agggagcgcc aatccagcgt
    18541 gaagcgtccg atctcagcct tgcctccggt ggcccgctcg acctcacgga tgatcctctg
    18601 cctcactttt tcccggaacc agccggaccg aagcacgagg acacccgaaa cacccgcgac
    18661 aagcaacaag gcaagcgcgc ccagtgacag caggagcagg cgtctgcgca gggggctcac
    18721 tgaaacgcct cctcgtgata ttcgatccgg atccggttac gcacctcttt gatccagttg
    18781 tcgagtgttt gatccacgcg ctgttgcctc agaatctcct ccactttgct tcgagcctct
    18841 tcgaaggaag gaggcttccc agctttatcc tcccactgcg gaaccagttt gtagcggtaa
    18901 tattcccaaa ggtccgcatc aagaatctgt accgccggcc gaaaacgttg gtcgatgaac
    18961 cgcagcgtgg cgagctgctt gaagagatgc cggacaacat cctcctcctg gagaccatat
    19021 tccgtcagga cccgccgata ctcgggttcg gatgcgaacc gttccttttt cacatcctcg
    19081 agtatccggc tcacctccga gggctcggga accggagact ggccggcatt catttcgcgg
    19141 ccgatcagcg cttgttcgac cagccgcccg gcggctttcc gcctggcatc cgcggtgaaa
    19201 tcgggcttat cgccgttcag gaaggcggtg acgcggatct cctcgagaag atcgctcgcg
    19261 gtgatcacat ccgtcccgac agtgacagcg acgcgatcga tgatctcagc gcgcaacagg
    19321 ctcagagaca caagaaccag aatggcgccg cggcgatgca tcagaatgtc tgccccaagg
    19381 agaaatggaa ctgaaagcgg ttgatccgtt gtgggacggc ctcgcaggtg ccgaacagga
    19441 gctcgtcgcg ggtcccctta cagccgatga aacgcggcga attcggcccg taggcaaagt
    19501 cgacgcggat cggaccgatc ggggtcctga agcggatgcc gaggccgatc gcatgcacca
    19561 tgtagtcaaa gtccgattca tcgcgctggc tgacccggaa gctcaggttt tcgatccggg
    19621 agtaaacatt gccggcatcg tgaaaaagca cacctccaat gttttccccg atgaggggaa
    19681 aacgaagctc cgtaccgaac acgagcaggg ccgagcctcc gagcgggaat ccagtgacgg
    19741 ggtcgcgcgg accggcctgg ttctccggaa atccacggtg ggtcgaggcg ccgccggcaa
    19801 aaaaccgctc gggtagcggg atctgctggt tggaaggcag gttcgggtct gtgctcaggg
    19861 tttgcaggta gccgaagagc acgctccggg caagaacgag tttgcggccc accggatgat
    19921 acgttgagtt gcggctgagc atgcggagga aatccgcctg cgaaccgaac ggcttggcgg
    19981 ctacgccgaa gtccactgaa ttgtatgtgc ccttgtggga atcggtcggg tcgtcacggc
    20041 gatcgtccac gtaagtgccc gaggggatgc cgacgcgcac cggttgggag agcagcggga
    20101 tgagttccgg gcggatcttg agcgtgtttt cgtcgatgga aacacggcgg aaggaatagc
    20161 gatagaacag cgttctcgat cgcgtccagc gctgggtaat ttgagccgag gtctcccagc
    20221 gccgcgagga gaatgtgcgg atgtcgcggg agtcgtcgta gagggcggtc agggaggcat
    20281 ctaaagtttc gtacccctga aattgggggg cgacatacgt gaccaggccg cggcgctgaa
    20341 tggtcgagac gcggcttcga aaactgactg tgtgggcacg gcccaggaag ttaagccggc
    20401 tgaagtcgaa cgagacgcga gggctgaagc cggcggatcc ggcaggagac tcaaggcttg
    20461 tctggctccc tccgatgcgg gcgatttccg cgccgaaacc ggtggtgatg gagtactttc
    20521 tcgattcgtc aagctggtac aggatgtact tgtacttttc gtcgccctcc gggttctgga
    20581 tggcggtgtc gactttcgcg aaaatgccga ggtcgtaaag gcggcgctgg gtgtcgagaa
    20641 tcttcgcctg tgagaccggc tcaccggggt tcagcaggat ctggcggttg accagttggg
    20701 gacgggtcgc gtcgaggccg gtgatgagca cttcgcggac gtagagccgc gggccctccg
    20761 taatcacgaa gcggaggttg acttggcggg gtttggcgga cggctcgaga ctccactcaa
    20821 acgccgcttt cgggtatcca ccgttgtagt agtaagcaag gatgttgtcg cggtcggcag
    20881 ccacattggc ttcgctgaac gcctgtcccg gcgaggattg gagcatcgac cggatcgtct
    20941 cctcatcttc ctgattgacg ccctccagct tcaaatcggc gacaaaccac tggtcgcctt
    21001 ccttcacgtc aacgaacacg gcgacattcc cgcgttttcc ccggtagtcg tcctggactt
    21061 ccgaggtgac gttcacatcc cggaagccgt tggtccggta gagatcggcg atggaggcta
    21121 gatcctgttc gagcatgctc tcgctgtacc gcccgcgacg gaactggagc gatgagggaa
    21181 ggacgtacat ccgctctcga atggttgcgg tatcgaagaa cgagttgccg cggatctcga
    21241 gatggaccag tttctgccgt tcaccccggt caatatcgta gacgatctcc tgacggccct
    21301 ggtttgcggg cagggaacga aacgagacgc gcgcgtcgaa atagccttta gtctgaaagt
    21361 actcggcgat atcacgcgtt ccctcgacca gcagatcgcg gtccacggag cgttcctcgt
    21421 agaccggaac gagttccttg aggcgtccac gggagacttt cgcaccctcc gttcggattt
    21481 cgacttcggg gccggcatcg acagtgaggg ccggcacggc gcgcccggtg ttggcgttat
    21541 gatcgagact gtcaagagaa acctgggcca tcaggtgatc cagtttctcg tacgactgcc
    21601 gaatacgctc taatccgcgc tgcacccgcg tggccgtgac gggcttccat ccaagccatc
    21661 ctttccatcg cgtggccttg atgatccgct cgacaggatg ctcaggattg ccgcggatga
    21721 ccggcacacc gaagcggtcg cggcggccgg gcgtgacttc gaagtggatg tgaacctgtt
    21781 gggtgcgggg atcatgctcg aagcgaggct caacggaagc accgaagaaa ccattgcgtt
    21841 gaagagtctg ctgaatgttg acgacagcgg cggcggtctg ggattccagg aactgatagc
    21901 caagattgag gtcggcgtcg ttcaccaact ggccgacgct gggcggcgaa ggaacacccg
    21961 tcaccgatac acggccgacg aaccagttgc tcttggtcag aaaacgcagg actaccttgc
    22021 cgtcgcgcag ttctgcgtcg acggctatgt cctcgtatcg cccggtcgcg tatagccgct
    22081 cgatggccgt ccgcaccgtc tccatcttga gcggggtgtt catcttgagc ggaagtatct
    22141 ccgctaactc ggaagagggg atcggttgtt cccgcggttc gaacgcgatc acggagatgg
    22201 gtttcgcttc gtactggtcc ggtgcggcgc gcaaggccac gcccagcgag cagaccaagg
    22261 ggaggaaaat ccaaaatggc aagccacaaa gccgcactat ctgcatgata cagcaagcgg
    22321 ttgatacgat tgttttgaat gacagcgcgg gaacgggaac gctactctcg gcagattctc
    22381 ttcaccgggc ttggcgaaac ggggcaggag agactgcttc gctcgcgggt cgcgatcgtg
    22441 ggttgcggag ctctggggag cttccaggcg gacgcgctgg cgcgagccgg cgtggggcgc
    22501 ctgaggatta tcgatcgtga ctacgttgag ctcagcaatc ttcagcggca gtggctgttc
    22561 gaggaagccg acgcggagga gggcctgccc aaggctgtgg ccgccgcgcg ccgggtggcg
    22621 aagatcaact ccggagttga agccgctccg gcggtggacg atctgacccc ggccaacgct
    22681 gaggaattgc tggggggcgt cgatctgatt ctcgacggaa cggacaattt cgagacacgg
    22741 tacctgatca acgatttcgc cgtccatcgc gaaatcccgt ggatctacgg ggcggcggtg
    22801 gggagttacg gcttgacgat ggcggtgttg ccggggcgta cggcgtgcct gaagtgcatc
    22861 tatccggaac ctcccggagg aacgcagccg acctgcgaaa ccgcgggtgt actgaacgcg
    22921 gtcacatcca cgatcgcagc gctacaaacc tccgaggcga tcaagatcct ctccgggaat
    22981 ctggaggcac tttcgaagcg gctgaccacg gtggacgtct ggagtggggt cgtgcggcag
    23041 atcgagcagc cggcgccgga tccgtcgtgc atggcctgcg gcaaaaggcg gttcgtgcac
    23101 ttggagggtt cgggccgagc gccgatcagc ctgtgtgggc gaaacgcggt gcagatccac
    23161 gaacgctcgc ggccggtcga cctcacggaa ctgaagcgcc ggctcgctcc gctgggagaa
    23221 gtccgcgcca acgagttcgc tctccgcttt ttcctgctcc cctacgagat cacggtgttc
    23281 ccggacggcc gcgcgatcat taaggggacg acggatacgg gcctcgcgcg cagtctttat
    23341 gcgcggtaca tcggcgcgta gccccggggc tactcccact ccacacgtgg aggagtttgc
    23401 atggactgcg ggctggtggc ggcgcccacg tgcaccggca tgtccacaaa gtgcagccag
    23461 aaagcgagag agcgggcgcc agttgacgcg aagtcccgcc gtatcgcgag ccggccacgc
    23521 gaattcacaa agatggcatc gctggtaacc ggtcgcgagc gggcgaaatc cgcgacagcg
    23581 accgcggtgg cacggcgccg gtccatcacg tgggcccacc cttcgacgtt gccttggcca
    23641 gcggcgtacg ctccgccggc gacgtcgatc cgccaggagg gcggtcctgt ggatgggaga
    23701 tccgcctgga acagggcggc gctgtcattc cgcaaggccg cgtaaaccat cgtgccagcg
    23761 ccgaaatcga ccagcaccgg atcgccgtcg agaagcaacc cgacatccgc ttccatcgcg
    23821 gcaaccaagg catccggatc ctcgacggtc caacgcacct cgacccagga cttcgagcgc
    23881 gggaattcca tttcgacggt ggtcttcaca gtaccggatt gaccgagtga ttcgtggccc
    23941 tcgaatctga gacacgcact caacggcccg cgcttgagaa tccgggaatc gagcggcgga
    24001 cggctggaac cgacttcgga ctccgcacca tctttagagc gcaggacgaa accacgcgag
    24061 ccgctgccca cgtaagtcag ttcagaccat cgcaccaaac tcaagaagcc acggacgtgc
    24121 tttgggatct cgaaaaccag tccagccggg taccgcacct ggaacgcttc cccgatctcg
    24181 acgacgtcaa taccagcgcc gggcgcaggg cccggtccct cttcgacccg gtaccggcga
    24241 acttcgaaag gtccaagact cacactgaag tcaatctcaa gcgcgccgtc gccgatgggc
    24301 gtgaactgcg cgggcacggt tttcccgtca tcgagcaggc gaagagcagc cgcaggcttc
    24361 tcgcaatgga agcgagcgcg aacagggtag ccgaatcgcc ggagccctgc cgtctcacgc
    24421 acgcggaatt cggccacctg cgcgcacaga gccgctggaa tcaagagcgc gatgagttgc
    24481 gcgaaactca tcgcgaggtc tccagaacaa tgatcgtgtc caggagatcg cgggcggaat
    24541 gcggcaatcg cagaatcgct ccgccgggga tctccttcca ttcgacggca gcgccagtcg
    24601 ccagcagctt cgcggacttc acgttcgcaa tattcggtag ggcgagaagt tcatccggcc
    24661 agtcaagcac gtgtacgaac actttgccct gcttctgcgt ggtgaccccc cagggacggg
    24721 gagtgacagg gccgccgcgg gttccgtaga tcgactcgcc attcttctgc atccactgtc
    24781 cgatttcccg gaggcgttcg acgaactccg gctggatctt tccgttgggc atgggtccga
    24841 cgttaagaag gaagttagcg ttataacccg ccgcgcgcac gaggtaacgg ataaggtcgc
    24901 tggtgttctt gaactgcttg tcggtggagt tgtagcccca ggaccggttg atggtctcgc
    24961 acgtttcgag cggcagttgt ccgattcccg cgtcggcgtt gaagcctgcg gtattgtggc
    25021 cgggaaggtc cttctcgaac atttggtagt cctcgccctc aaacggcatc cggtggtgat
    25081 tgttgccgat gagcgtcgca ggttggagtc tgtgaatgag ggagtaagtt ttgttcagac
    25141 gccattcggc atccggcctg tcccaccaac cgtcgaacca gatgccgccg atttcgccgt
    25201 aatcggtcag cagttcgcgg agttgggcgt ccatgtaatc gaggtaccgg ttgaagtccc
    25261 cattctcagg cctgccgctg ctctggcccg tgcggccccg gggatagtag tccggatggt
    25321 gccagtccag gtgtgagtga tagaagaaaa gcttgatgcc ctgcttctta cattcatccg
    25381 ccagcatctt caagacgtcc ttcgtgtacg gagtggcgtc gacgatgttc cacgtgctct
    25441 gcttcgtgcc ccacatagcg aagccgtcat gatgcttgct cgtgatggtg atgtatttca
    25501 tgccggcgtt cttggccatc gacacccact gtgccggatg gtactcggtg ggattgaact
    25561 tcggggcaag cttctcatac tcggaaatgg gaatcttctt gttgttcatc acccattccc
    25621 cgtcgcccag gacgctgtag acgccccaat ggatgaacat tccgaacttc gcattctgaa
    25681 accattcccg cgccttcagc ctttccggcg ggaccttctg tgtctgggcg acggccagaa
    25741 cggcgaggga gagaacgacc accagctttt tgagcacaat gactccttgg aagaatctgt
    25801 ccttcttatc tatcacatcc ttgacagggt gctgtagctt ggcgttgaca gctccctttt
    25861 ggccgcggct cgaaatggtg ctcgacctct gccgatgttg aaacatgaca aaggcgtagg
    25921 ggaaactggg agcgtgcctg agttctgtac atgtggggcg gaacttcctc ccgatgcaag
    25981 attctgccat aagtgcggca agccgcaacg cgaggagcct cagctttcgg aaccagtcga
    26041 gaacagtttg agcggcgaaa cacccgtgga tgccgagccg ctgccagcac tccggccgcc
    26101 ggtgatcaat ctccacaacg gcctcgcggt ccgggtgggt tttctcgcgg gcagcatcgc
    26161 gttcgtcctt ggtctgttac cagcgccatt catcggacgt gtggcgctgc tgttgttcgc
    26221 cggattcctc tcggtgtatt tctaccggcg gcggagcggg gagtcgttgt cgatcgggaa
    26281 cggcgcgcgt atggggtgga tcagcgggct cttctgtttc gtcatcatca ccgtgctgtt
    26341 caccatcaat atggtgatga tttcgattgt gagccaagag ggaggcctag ctgccttcta
    26401 ccggcagcaa ctcggcgcca tgggcatgcc ggcacagagc atcgaacagg tgattgaagt
    26461 tttcggcagc ccgttacaga tcaccgggct gttgttcacg cttttcgtga tgttcaccgg
    26521 cctgctcacg gcgggcgggg cgctcggagc gagattactc aggcgcgact agtccagttc
    26581 gagcgccgtg ggcggacggc tgtagcgttt gacgagcgcg acaccgacga agatgaccgc
    26641 catcgccagc gcttcccgcg cgccgaacga ttcccggtaa aacagccatc ccagcaggac
    26701 cgcgacgatc gggttgatgt aggtgtagat cgacacgagg gccacgggca gtttctccag
    26761 agcgtagaca tacgaactgt agcccacgat ggagccgaac accacgaggt acagcatcgc
    26821 cgcaaccccc cgagacttcc agacgacggg ctgagacggg atcaacaacg caggcggcag
    26881 aaaggccagc cccgccgtca gttgctggat tgccccgcct atcatcgggt gcgcctgctt
    26941 actcatgcgc cgctgccgta tggaaccgta agaccaggcg aggcaaccga attgaagcac
    27001 caagaacccc cagagtagcg gccctcgaag tccaccaccg gagccgggcg ccagtaacag
    27061 cgccgcgccc aggcacccaa ccgtgaggcc ggcagtggtg ggaaagtgga gccgctcgcc
    27121 gcccggcaga agcgcttcga cgccaaccat ccaaaaagcg gacgtggtaa cgatgagagc
    27181 ggccaggccg ctggggatcc acacttcggc aaaggtcagg caggcgttcc cgatgccgag
    27241 gataagcaag ccattccagg cggtctgcgc cagttcgcgg ccccgaggca acggcacccc
    27301 gaggattctg cccgccagga gcatgatcga gccggaaagc agaaagcgtg tactcacaag
    27361 tacaagaggc gggaaggact ccagcgccat gcggatgccg agatatgtcg tgccccagaa
    27421 gaagcacacc gacgccaacg ccgtgtaagc cttgaaatgt ggatgatggc gcaagagttc
    27481 agggtagcat cctgccggat gtgcgacgag agagggcgct tacgggggcc tgagctagaa
    27541 tgaagtgtga ttcttaccct cacggtaaac cccgccatcg accggaattt cactgctgac
    27601 aggctggttt tcgaagatcg agcgtacatc ctgtcaagtt cgatgtccgc gggtggcagg
    27661 gggatcaacg cctcgcgagt gatccactca ttcggcggcg agaccacggc aattgtgacg
    27721 tccggaggaa agccgggaac aaaatttgag cagctgcttg ccgacagcgg gtttccggtg
    27781 gaagtggtgc ggatcaagca ctggatacgt aacaatctga cggtcacgga taagaccggc
    27841 ctgacgatca agttgaacga actcggaccg acgctggaca gcgccgaact ggggcgcatc
    27901 gagcgggcag taagcaggaa actaccggga gcgaagtggc tcatgatctg cggcagcctt
    27961 ccgcccggcg taggaccgga gttctactca aagctgatcg ccatggcccg ggaaaagaac
    28021 gtgcggacgc tgctggacgc ggacggcgag gctctgcggg agggagtgga ggccggtccg
    28081 accgtcgtca cgcccaacca gcaggaggcg gagcggcttc tgggcgaggc tctgattacg
    28141 cgcaagcact tcctggaagc agcggaccgg atccggcgca tgggggccga gtcggtagtg
    28201 ctttcgcttg gaaacagagg cgcggtcggc gcacgagcgg gatcgaccat agaagtcatc
    28261 gcgccacgca ttgacgcggt atcgccgatc ggcgcggggg atgcgatggc ggcggcgttc
    28321 gtctggtcga tgtggcgccg ggatgatttt acggacgcgt tgcgctgggg agtggccgcc
    28381 gggacggctt caaccaagct gccgggggtg accttcgcct cgctggaaca aaccaaggaa
    28441 atctaccccc aggtggagct acgcacggtc gcgtaaagcg cggaggggtc aggcgtcccg
    28501 aagcccctcg cgggcacgat caagaaggat ccgggacatc gccgtgtggc catccagggg
    28561 cgcggtgaca acgacggaaa gaccggggtg ggccgcctct atctctccca cgagcgccgg
    28621 cagatcctga gcagcatgcc tccccagcgt gagaaagtac gggatgacca caactcgctc
    28681 ggcgccgaga ccgaccaggc gctcaatggc ccccttcaag tctggttccg ccaactcgag
    28741 gaaggcagcc tcaacgagct catagccgcc cagaccggcc agttcggccg caaccgcacg
    28801 cacagcctcg ttggctgatt ccactctcga cccgtgcgcg aaaacgacga tgcccgtctt
    28861 catcctacgg agtcaccagc ggtgaacttc cgcccagccg gccggcaggc tgggggaggc
    28921 cctggccttg cgggagggac gtacgcagaa agtccaggaa tgcccggcct ttccgccggc
    28981 cgttgttgtg tccgatctcc agtctttgca ggaacaccag aatcccgaga acatccgcgt
    29041 cccggaccgt ctcgatcccg actcgctcac gatgttgctt acgatactcg tcgatcgcct
    29101 ggcgaaacgc ctgctggatg gaggcggcaa caaggttgga gggcctggtt tcgtaaacca
    29161 acccgctttc gagagttcgg tgggtgcgga caagagcgtc aagcgcctcg cggacgtcga
    29221 aatcgaccgc accggctgtg tctgccgtgg cctcgaacag cgcgcggacg gagacggcga
    29281 gcagttcttc gtgatcgcga aggaagctct ccgacacctg aatgtcccgg ttggggaccg
    29341 tctcggggtc gatatcggga ggtctttcgt gaaggcgggc ctcctggagg tactcgcaat
    29401 ccagtgggca gtcaacggaa acttcccgtt cggttccgca acaaagcgag cagatttcac
    29461 cacgcactcc cggacagaaa cggcggggac gccttgtttc gcagatttgg cagtttctca
    29521 tgaaattcgt gggacgccgg tgcgactgtt tcccttcatc gtagcacttg cgggacgcgg
    29581 tccggccgct actctggctc cgcgacagtg cgctggaagg tccgcttcag gaagccgtcg
    29641 gtcatgccgg cgatgtagtc gcatacgacg cggtggacag gtccttcacc cgcctgctca
    29701 cggtaaggag aaggcaagcg gtccgggtgg cgaagaagaa actcaaacaa gccggacagc
    29761 atcgccgtgg agcgttcgcg ctcctcaagg atgccttccg aggcgtagac gttacggcgc
    29821 aggaagtcct tgagcgccgt gtcggttgcc cgcgcctcgg gcgtgaaaca cgcgagcctg
    29881 cccgggaaac ggcggacatc ctccacgtcc cgaagacggg cctccgaggc cgcggcgaac
    29941 gttccctcga tcaaaccgga gaccaacaga tcgatgagcc gccgcagcgc ctcgagaaaa
    30001 tggacccgct ccgacgcacc cgggaactgg ttggaaacgg tttcgaggat aaccgagaag
    30061 gaaggcacag atgcagcgac actttcgacg gaaaacagtc ccgccgcgaa gccatcgtcc
    30121 aggtcggctg tgttataggc tatctcgtcg gccaggtcga tcagttgcgc ctccagaggc
    30181 gggcgcaggc cgggaagata gtcgtcgagg tcgggattct cgcccggatc gagatcgcga
    30241 gaatgtttca cgattccctc gcgaacttcg aaagtgaggt tcaagccggg aaaccgtgcg
    30301 taacgctgct cgaatgattc gacgatgcgg agggcatgca gattgtggtc gaagcgctct
    30361 ccgaactgcc gcatccggcg atcaagttcc tcctcgccgg catgcgcgaa cggcggatgc
    30421 ccgatgtcat gggcgagtgc gagcgcctcg gtgagctgct catcgaggtc gagcacagtg
    30481 gcgaccgtcc gggagatctg agaaacctcg atcgtgtgtg tcaggcgatt ccggaaatgg
    30541 tcagacagcc cggcggagaa cacctgcgtc ttgtcttcca accggcgaaa ggcgcgggca
    30601 tggacaacgc ggtcacggtc ccgctgaaac tcgttgcggt agggatgtgg cggctcggga
    30661 taccggcgcc cgcgggtttg tccaaaaggg aatcgcagcc cgcggaggct cagtttcatt
    30721 tcctactgct taggcagttc tccgagaatc gtaagggcaa cgcggctgac ggcgccgggt
    30781 tgcgtacgca ggggctcgag gagctttcgc gcctcgtcgg gccgggaact ggctagaagc
    30841 cgcgccagca tgatgatggc ctgctccttg gagacgaaga tcgtcggttt tgccatgagg
    30901 gcgcgaagca gcttctcagc ctccgccgtc ttaccttgca ggtggtagac ctgcgcgaga
    30961 gagaggttcg cgagggaggc gtactcgggc gatccagact cagccgccga tttcagccag
    31021 ttggcggcgt catcgagccg cccctgatcc gctgctacag tgccgagata gtactgggca
    31081 atggcgcctt cttcggtacc ggagtggcgc gaagcgagtt cggtgaaagc cttgatggca
    31141 gccttgttct tctcttcaac cgtcggaaag gtcagaatgg attcacgccc agtgggcggg
    31201 ccaacgcctg cgtcctgaat acgggtcgcg gcggcgagtt cccgctggcg gagggcatgc
    31261 tgccgcttgc tgtagtagaa gtagccggcc accagcacca gtactacgag cgctatcgag
    31321 ccaaaccgga tgaattgcct gcggtgctcg gcgacgtact cgacggtgtg gccgacctcc
    31381 tgggcaaaga cgtccttctt gagttccttg cgagtgagtc tgtccacaaa aacctctggg
    31441 agatctatga tcgtagcaca atcgcgtttc agcggcccag actcaggaaa ccgatgtgag
    31501 attcttgcgg acggcgaact tcaccaggcc ggcaagatca tagatgtcca gttttcgcat
    31561 caggctgcgg cggaaagtgt cgacggtctt cgggctcagg tccaggcggg cggcgatctc
    31621 cttggccctc atgccctcca ccagcagcga aaagacctgg tgctccctga ggcttagggt
    31681 ttcgaacggc gcagttgtac cgctgcactt tcgggggata aagatttcgc cgagtgtcac
    31741 ctggggtgac acgtagacgc cgcctccgag gaccgagtga aacgcatcca aaacgcgagc
    31801 agcgcaatcg gactttgcca ggaatccatt cgcaccgtgc cgcagagccg caattacggt
    31861 tttgcgatcg gtcttctcgg acatcaagat cagccgcgat ggcacatttg cccggaccgc
    31921 tttatcgatc acggccaagc cgaacaactt cggaagattc agttgaagaa gtgcgatgtc
    31981 gggacggagc gactcaagca ttctgagcgc cgacaagccg tccgagcagt gtgctacgac
    32041 acggaacggc gaatcccgtt cgcagatcgc ggctatacct tcacaaagaa gttcccactc
    32101 gtcggccaca acgacacggg caattccctt ttctttcatt gccgcaaggc cctagcagcc
    32161 ttcgcgtccg gggcggaatc acatcgtgtc cgtccctggc cggacgaggt caccctcatt
    32221 ccttctgttg tattcgcctc ccgtcgaaca agccgaacag cgtcgccaag aaaacccccc
    32281 cgagcccaac ctgggagccc agatgccatc cacgtcaggc gaagctgcgg atggcgctgg
    32341 tctcgtcgtc gtaaacgtcg aacaccgtgt agagtttcgt gatctgaagg aggtccttca
    32401 ctctcttggt gagaccgagc agcctgagaa caccgccctg gttcgtcacg gtagtgaagc
    32461 ctgaaacaag ctcaccaatg cctgagctgt cgatgtatga aacatcgctg aggttcaaca
    32521 ggatcttctt gttgcccttg gcaacgagat cgcgaatcga ctcacgaagg gtgctggccc
    32581 cttcacccag agtaatgcgt ccggttacgt cgatcacgct gacatcgcca acctggcggg
    32641 ttgtcacttg aatactcact ggaacaatcc tcctcgctga gaactcacaa gaatgcttgt
    32701 caatcgtgcc gcaagtactt taccaacttc acttccgttc ccgccggtac caggcgctgc
    32761 atctggtaat catccacaaa cgcccggatc aggaggatgc cacgtccgga ctgctgcagc
    32821 aaattctcct cggcgagagg gtcggggatt gacttgggct cgaatccatt cccttcgtcg
    32881 gcgacggtaa tggtgattcg atcatccccc ttcaggacct gcagatgcac ccgcttgcgc
    32941 gaattgtacc ggttaccatg aaccaccgca ttgacgaccg tttcacgaac ggccataccg
    33001 atcttgtgca gatcgtcgtc gccgaaaccg acctcctcgg ctgctttcag tacaaggttt
    33061 tcggcgtcgt caacgctatc cagcgtggaa gacaggaaga cgtccacgtt tccgttctcc
    33121 ccgctcagaa tttctcccat aaccctctac acgaccgcta cggggcgatc agttcagccg
    33181 aagtcgaacc gttacaatag aatagtagcg tcgcaaaagt caagatgcag ctacctcggg
    33241 cgaggatgcg aggctcttgc agggcgggga gaccgttcgc cgccccacgg cggcggtgac
    33301 tcttgtggag atctcatggg agctttgctg gttgcattgg tattatccct gcccgtcacg
    33361 gcgcaggatt tctccggcgt tcgagtagag aagatttcgg ggggctatgc cttcacggag
    33421 ggtccggtgt ggtcgcgaga cggttacctc ctcttcagcg acgtcccgaa taacaagatc
    33481 atccgtttcg agcccgggga gggcacggct gtcttccggg agaattccga cggcgcaaac
    33541 ggcaatacgt tcgatgcccg gggacgcctc tacacctgcg agagcaggac acggcgggta
    33601 acgcgaaccg gcaaggacgg caaaatcgag gtgctggcag aaaaatggca aggcaagcgg
    33661 ctgaatgcgc cgaatgacat cgtcgtcagg aaggacggcc acgtctattt taccgacccc
    33721 gcgttcggac agcaagggga cacgcgcgaa ctcgacttct ttggcgtgta ccacatctcc
    33781 cccaaaggtg agatggaggt ggtcgcaaag ccagccggcc gacccaacgg gatcgccctt
    33841 tcgcccgacg gcaaggtcct ctatgtcgcc aactcggacg accgaaacct tcgcgcgtac
    33901 gacgtcgacc ggaacggcaa ggcggccaac gagcacgtag tcgtatccag gatcgacggc
    33961 gtgccggacg gcattaaggt cgatgagaga ggaaacatct acgtgaccgc ccgaggggtc
    34021 gccgtctact ctccgggtgg aaaactgctg acgaccctcg agttcacaga gacacccgcc
    34081 aactgcgcat tcggcgatgc ggatttccag acgctgtacg taactggccg tacatctctc
    34141 taccgcgtgc ggctcacggt aaagggagct gtgcagtact aggccggcgg catgtcgagg
    34201 gttgcaagtg gtccgcggat gagtccagga gtggagatgc atggccgtta aggggcggcg
    34261 gtatcccgag cgaccgatcg tgggggtagg ggcgattatc ctgagacgcg atcgagtgct
    34321 cctggtcgaa cgggcgggtg aaccgttgaa gggctactgg tcgctgccgg gcggtgtcct
    34381 cgaagccggc gaatcgcttc acgacggcgt tcagcgggag gtgagggaag aaaccggcct
    34441 cgaagtcgag cctttgggaa tcttcgaggt cttcgagcgg atcaaccgcg acgagcgtgg
    34501 gcggaccgaa taccactatg tgctggtcga ttacatgtgc cgggtgaccg gcggcgagct
    34561 ccggccggcg gacgacgcaa gccgcgcaga gtgggtccgg cgtgcggacc tccggaagtt
    34621 ccagattacg gaaggaaccg aggatgtcct cagaagagcc ttccagcagc gacggagagg
    34681 actcctcaag ccatgaaaac cacaagctca gacctgctcg aattcgaggc cctccgcgag
    34741 ctcgtcgggc gctacgtcgc gagcccgctt ggcctggcgc tgctccgcga ggcagctccc
    34801 catacggacc gcgggcacct ggagcaggcg ctcgccgacg ttgccgaggc gatcgagtac
    34861 gtgcaagcag cctccaagcc ccatcccgcg gtacgtggag cggcggtccg catccggttc
    34921 gactctctga gcgacccaac ggaagctctt cagaaactgc ggatcgaggg gacggttctg
    34981 gaggcgaagg agattctcca actcaccggt ctgatggacc tagcgtcgga tgtaaagcgg
    35041 ctgctcgggt cggttgccga acgattcccg cggctggccg ggagaggccg cgaaatcgga
    35101 gagttccact cgctggtccg cgacctggtc ggaaaggtcc tgccggacgg cacactggcc
    35161 gatgacgcaa gcccggcgct ggcgcgcctg cgccgggata tcgacaggca gaggaagctc
    35221 gtccaggagt cgctcgagag gtttctgcgg atccaccgcg aggagaacgt ccttcaggaa
    35281 gagttcgtca cgatccgcaa cgaccggttc gtggtaccgg tgattgccgg gcgccagcgg
    35341 aagatcgaag gcgtgatcca cggagccagc ggcagcggcc acaccatgtt cgtcgaaccg
    35401 ctcgaaacca tcgaattgaa caacgaactg gtgcggctca gcgaagagga actccgcgag
    35461 attcaccata tccttcgcga gatgaccgca agcctgcggg caagtggtga ggccatcgcg
    35521 gcaaccgccg aggtcctggg agatctcgaa ttcctgttcg ccaaggcgcg atttgcatcc
    35581 gatttcgact gcacggtgcc gcgattcagc ccgtccgagg cacggcgcct cagtcttcgc
    35641 gaggcgcgcc atccgctcct agcggatgtc ctgcgccgtc agaagaagcc gatcatcccg
    35701 gtgtctcttg aattgacgga agactgccgg accctcttga tcagcgggcc gaatacaggc
    35761 ggcaagacgg tcacgctaaa gacggtcggg cttctcgcgc tgatggcgca ggcgggcttt
    35821 ccggtaccct gctccgaggc ggagtttcca ttattcggcc aggtgcttgc ggatatcggc
    35881 gaccagcagt ccatccagga aagtctgagc acgttctcgg cgcacatctg ccgcgtgcgc
    35941 gacatgctcg acgcggccga ttcgacttct ctggtgttac tcgacgaact gggccgggcg
    36001 acggacccgg aggagggagg agcgctcggc gtcgccgttc tggagcgctt cggctcagcc
    36061 ggtgcgttca cgctgtcatc cacgcatctg atggctctga aggtgtacgg cgcctcaacg
    36121 ccgggcgtag tcaatgcgtc gatgggattc gacgacgaga cgctcaggcc gacgtatgtc
    36181 ctgaaggtgg gcgctcccgg caagtcagcc ggtctggaga ttgcgaagcg cctgggaatg
    36241 cccgatgtgt tgatcggcga ggcaggccag cggctcggaa gccgcgagcg cgatctcagc
    36301 agctttctct ctgacttaca ccgccggctg gaccgcctgg ctgcgcaaga gcaggaactg
    36361 cgggagataa aggaagccct gatcgcccgt cagaaatccc tggcaacgga atgggagcgc
    36421 cgggaatccg caaagctgaa agaactggag cggcgctacg agttggtgct tgagaagttc
    36481 gacagccagg cgcaggaggc gatcgaacgg attgtcgccg aagccgaacg gaggaaggtt
    36541 gcttcgtccg ccatgcgtcg tgtcggaggg ctgaagcgcg gacttcggga agagctggaa
    36601 gtcactgtgg tgtccacacg gaatgagtcc cggcaaggcg ccctcgaaac accgaaggcg
    36661 caaattacgg aaggcgtgcg ggtgcggctc aagaacatcc gcgaacccgc acgggtcagc
    36721 cgaaagctgg caggcggcct gatcgaagtc caggcgggct tcctcaagct tcaggtttcg
    36781 gaggatgacg ttctggaagt cctgccggat gccggagggg gcgcgggcct gccgaggcag
    36841 gtcacttacc gcccggcggt acaagcggcg caagctgtgc aagaaatcaa cgtaatcggc
    36901 cagcgtgccg aagaggcatg cgatgcggtg gagaagttcc tcgacaacgc ggtgctcgcc
    36961 gaggcgacgc gcgtgcggat tgttcacggg cacggcatgg gaattctgcg gcgggccatc
    37021 gctgagctgc tagccaaaaa tccgaacgtc gagaagttcc atgccggcga tcagttcgaa
    37081 ggaggcgccg gagcgacgat cgtggaacta aagggctgac agggcttcga gcacctgtgc
    37141 ggcgtgccct tgcgccttta ccttcggaaa aatcttcgcg atccggccgt ccttcccgat
    37201 gatgaaagtg gtgcgttcga tgcccatcac cgtcttcccg tacatgttct tttccttcca
    37261 cacgccgtaa gcctgagcgg ccgtcttgtc gacatcgcag aggagcgtaa aaggaaggtc
    37321 gtatttcgtc ttgaactttg actgggccgc caaagtgtcg ggggagattc caagaatcac
    37381 tgcgttatgt ttcgcgaact gcttcgaggt gtcgcggaac tcgcatgcct ccgttgtgca
    37441 gccgggtgtg tccgccctcg ggtagaaata gaggactaca gttttgccct tcaggctggc
    37501 gaggttgagg gcctctccgc gatctgtcgt gaggacgagg ggaggggctg gatcgccttc
    37561 tttcagggcg acgggcgctg ttttggccat aggtcttggg tgattactcg actgggacgg
    37621 ttgtcccctt tttttcttcc cgcgtgagcc actcgatgcg ctcatcgatc actgtccgga
    37681 tggctttcag gaactcaacg cgggcgttcc gaaagtgctc gcgagcagat tcggagccgc
    37741 actggagcat ggtggtcaac ttcggaccgg cacccatgca aagacagttc acacgaggct
    37801 cggtggaacc cgaagccgtg gtagtttcct tctcgctcat gaggcgacct ccttcatttc
    37861 gacggtcaga atcttgttgt ccagcctggc acgggccggt gtcaaggccg ccatcgaggt
    37921 tggcaggccg atatgccggc ggagtgtgcc gatctctacc accagttcgt cacccttctt
    37981 gaacagtccg atttcaccct tggtggcgaa gggcagatga atacggattt cgtagcggcc
    38041 atcgtacttc tcgaagctgt acggccgctc tgtgcggatt actgcggcgg gattctcgcc
    38101 atccggatag aggatcgaag caagctcttc caggcgcggc cttccgagca cttcgcgcgc
    38161 aaagagcgga acttttttca cgcgcaccgg agcgaagtac tcctcaatct cacggaggtt
    38221 ttcttcttgg gacacgcgcc agtcgtcaaa atagctgtca gtgacctcgc ttggtagtac
    38281 ccggttcaca atcaccgtgt ccacggtgag tccatgaagg gagaaataga cgaacgcgcg
    38341 ctgggtctcg cgcagtacca tcttctccgg gtttgttgcc agccgcacgg tcgtgaggcg
    38401 cggatcctcg agcagttcgt caataccctc gagcttccgg aacagctcct ggacgttggc
    38461 aaagtagctg tcagttggaa gctcaaccgg cgaaacgtga cgagcgagcg gccgcaccgc
    38521 cttgaggaga ctgcgctgga aggggaacac gtgcttcatg taccagtcga gcgtggtcgg
    38581 catgctcacg aatcgcatgg actcggcggt gggcgcgcag tccagcacga cggcatcgta
    38641 aagcttctcg cgccagtact gattcacgta aagcatggca ctcagctctt ccatgccggg
    38701 gagaatcgcc aactcctccg cttcgacgcc gcttataccc gtggtgcgca gcactgagac
    38761 gacataggag gagatctcct gccagtggcg cctgatctcc ttctggatgt tcacttcctg
    38821 gatggccaag tggctgttaa ccgggaatgg gtcggcggtt tgtccctgga agaggccggt
    38881 ggccagatcg aaagagtccg ccaggctgtg cgcgggatcg acgctcatca cgagggttcg
    38941 gagcccgagt tgcgccatcc taaccccggt ggccgcggca aggctagttt tccccacgcc
    39001 tcctttccca gtaaagagga ggatacgcat acccttattc tagtcctgcc aagagggggc
    39061 ggttcttttc aatccacgcc gcggtgtgcc gcggcacttc gggaccggtg gcgagtcgtg
    39121 ccaggtcagc aggtgtatca acgtcgaacc aagcggggcc gaccgcggtg gtaagaccac
    39181 acttccaaga ggcccggagg gtgtcttcca gggtgaaagg accagaccat cgaacgccgc
    39241 gaaacatgtc cgaatgggtg cggcggcagg cgatgacgca gtaaccgccg tcatcggtcg
    39301 gcatcagggc cacgtcagcg ggcaatgaca gcactgtttg caggtgtgac gtgggcaagg
    39361 tgggggcatc cgtcccgacg atcaccgctt gcgggcggtg ctccgagagg gctgtgttca
    39421 gagcatgcag catcctgatg ccgaggtcgc ccttctcctg caacctcctc gcaacctttg
    39481 tccccggcca ggcatccgta ttcatgttgg tgtgcaattc gatgtcggcg acgcgactca
    39541 ggacggcgat ccgttcgagc atgtctgcaa caaacgcctt gtgcaaaagg gcggcctcac
    39601 cagcccccag acgctcggca agcctggtct tgacctggcc ggccacgggc gccttggcga
    39661 agaggataat gacgggccgc atgaagtgat tgtagactgg tgttttcggg cgattgtttt
    39721 cgatgagggg aagaatgttt aaaatcggtc gttttcgtct gtttgcgatc tgcgtgtcgg
    39781 ccgccctggc ggccggagcg gcgattgccg cgccaaaggc tggaaagggc gacgcaggca
    39841 aggggaaggc tgtttttgag cagtgcgcgg tgtgccacaa cgccgacagc accgacaaga
    39901 agatggggcc tgggctgaag ggcctcttta aacgagcgaa gctggcgagt ggcaagccag
    39961 tgaatgaaag taacatccgt gcgaccatcg acgcgggtgg taacggaatg cctccctaca
    40021 aggagatgct gtcggatcag gagaaggaca acctgatcgc gtacctgaag acgctctgag
    40081 gtttccgtcc agctgaacgg ccgcggaccg cggttgacga ggccgcggcg caacgtctac
    40141 agtgaagaga gcctgccgag caaccggttt cacgtcccta tcgtctagcc cggcctagga
    40201 caccgccctt tcacggcggc aacgcgggtt cgaatcccgc tggggacgcc actcaaatca
    40261 agcccaatca gcgggttgtc ttggggagga ggtgcgcact gcctctacgg ctgaccctgc
    40321 gcctgccact ccgcaagtct ttcgcgagcc aagccggacg aggttgagct tgggcccagc
    40381 tcaagcaggc ggcgatactc gaccgccgcc gacgcccggt caccggccgc aacatggatt
    40441 tccgcgagca tggcgtggac ttccaggtag cggcggtctt cgcctgtttc gagaatggcg
    40501 tttattgact gcttcgctcg gtcagggtta tgcagcgcca tgttggctag gacgctgtaa
    40561 tagtgggcct ccgtcagcct gggatcgagc ctgagcgcgg agtccgcaag acgggccgct
    40621 tcagcaaagt gcctggattt caactcgatc acggccagcg acacgtacgg attcggaaat
    40681 ctgggatccg cggtgacggc gcgctcaaaa gccctgcgag ctccatcaat gtcatggcgt
    40741 tccagcagcg ttccccccaa gaaataccag gccgcagcga attgcggata gacgctcagg
    40801 gccctctcca gctcttgtga cattttgacg aggtttggcg tctttctgcc ggcctcgcgg
    40861 ctggctctct cgtaagcctt gcgtgcttcc ttcggtgccg caagagtcgt gaagctcaca
    40921 tagaactcac tcgcaacagc ggggggttgc gcgcgtacgg caccggctcc cagaaggaag
    40981 gccagagtca ccgaactcgc tcttttcatt gatgttcctc cgagtgaaac tgtcccgagc
    41041 ttagggtctg cgcaacctgc cgacaagcga caacgtgcga cgcacgtggg tgtcgccctt
    41101 tgtcgtgatt gcgggcgcgc ttgatgacct gcctgaccgg ggctaaaatt gactgagcct
    41161 gacgaggtgc cagtgccctc catcgagccg caaagagacc ggcgactgga ttcgtggaaa
    41221 gagatcgctg cctatctcgg ccgcgatgtg cgcaccgtgc agcggtggga gaaagaggag
    41281 ggcctccccg tccaccggca cacgcacaag aagctgggaa cggtctacgg gttccagtcg
    41341 gaaattgacg cctggtggga gagccgccgt gccagcctta agtctccgca accactcccg
    41401 aaacggtctc ggagaacatg gttggcggta ggagccgcgg ctgctatcgt tctcggcgcc
    41461 ggcgtcgtgt tggtaatccg attcatcact cctgagcccg ctcctgtaca gttcaaagcc
    41521 gtcccgttca ccacctaccc cggtgatgaa ctcggcgcca ctttctcccc ggacgggaac
    41581 caggtagcgt tcgcctggaa cgggcaaaac caggacaact atgacatcta cctcaaagcg
    41641 ttcgattcgg agaccccata tcggttgacc accgaccctg aggcggagat gttcccggct
    41701 tggtcgccag acgggcgcag aatcgccttt gttcgcagcg cggcgccttg gaaactgccc
    41761 gctgcgatct atgtgatacc cgcgacgggc ggcccccaaa ctcgggttgc ccagctctcc
    41821 accgtggagt gcttctttcg gaacgagagc ctgtcatggt ctccggatgg gaagtggctg
    41881 gcgttcgctg acggcgactc ggctgaggcc cagtccggaa tccacgtgct atcggtcgaa
    41941 accggcgaga cgcgaaggct cacacttccc cctgccggat ggggcggcga cgagctaccc
    42001 gcgttctctc cagatggccg gtcgatcgca ttcgcccggt tcaggagctt gacccgatct
    42061 gatatctacc tggtgtcgtt ggacggcggc atgcccagac gcctgtcgtt cgccaaccag
    42121 ttgattcgag gactggtgtg gacacctgat ggcaaggcga tcgtgttctc atcggacgga
    42181 ctgtggaaag tcgctgtcac cggaggttct ccggaacgcc ttcaaggaac aagcgacttg
    42241 gcagacgccc cggccatctc gccacagggc gggcggctga tcttctcgag gccggtctcc
    42301 aatgaaaaca tctggcgcgt cggcgtccag ggttcgcggc tggtgggaga gccgacacgg
    42361 ctgattgcct ccactcgaga cgatacgaac ccccagtatt cgccggacgg caggcgaatc
    42421 attttcgact cgaaccgctc gggaaactgg gagctctggg tgtgcaacag cgatgggtcg
    42481 aatccgattc cggtgagccg agccgggtgg ccgagctggt cgccggatgg caaatacatt
    42541 gctttcgacg acgatgtcaa aggcagttcg gacatttacg tagtcagcgc cgagggagga
    42601 acaccgaggg cgatcacgca ccggggtgaa aacgccgggc cgagctggtc tgccgacggc
    42661 tggatctact tccagtccaa tcgctccggc agcctcgaga tctggagaac gaggcccgac
    42721 ggcggtgagc aggttcaggt gaccaggaaa ggagggacga ggccggctgt tgcgcccgac
    42781 gggaagttcg tctacttcgc aaagggagtg ccggcagaag ctatatggcg gatcccggcg
    42841 gagggaggcg aggagaccct cttcctcgac aatgcggatg aagcggacat gggacattgg
    42901 gctccggtga aggaaggctt ctatttcgtg ctgcgcaagg aacggaggaa ggctctcgcg
    42961 tttctcgact tcaagaccgg tggcatcgcg gaggtgatgc agatcgagaa gccatgggac
    43021 cactccgccc tctccgtctc tccggacggg cgctcgattc tgtaccctca gttcgacgaa
    43081 atcgggcacg acctgatgct ggtggagaat ttccggtgaa gggtgcgcct cagggcggca
    43141 acggagtgct agtctaagag gctggatcct gctccattcg attcgaagtc gccaaaactg
    43201 tgagcaaaga aacgattcaa gcctacgacc tgccggcgcg ggtgtcgtcc tatgacgccg
    43261 acatggcgct gatgcacccc aaccgttcac ggatggtcga catcgccctg gaggtcgtgc
    43321 cttttgacgc gaaggccgcg ttcaccgtgt tggatctcgg tgccggcact ggattcttca
    43381 cccaggctgt cctgcgccgg tttccgaact gccgggtgat cgctgtggac ggcgcagctt
    43441 ccatggtcga ccttgccaaa acgaggctcg gcgcggaggc atcgcgcgtg gagttccgcg
    43501 tcggagactt ccgcgagttg tcgcgactca tcccgaacga acatggcgag tttattttct
    43561 cctcgtacgc gcttcaccac ctgacgccgg aggaaaagac cggcgtcata agggacgcac
    43621 tctctttcct gaaaccaggg ggatggttcc tgaatgcaga cgttgtgacc gcgggacacg
    43681 agcggacaga ggcgcgcatt caggacgtcc gcattcaggg tatcgtccaa cgggcctccg
    43741 gccgggacga gcgctttccg gacgctgtct cggtcaggac gttcctggat gacctggaag
    43801 cccgcgacca cgacaagccg ctgccggtaa ccgaggaact acgtatcctc aagcaaggcg
    43861 ggctggcgag cgcaacggtg ttctggctcg agtaccggga ggcagtgacg ggcggagtga
    43921 cgtaggccgt tggcggggcc gccattgcta tgatccgtat cctatgggct ctcgcgtgcg
    43981 ggtaccgcac ctgatcggcg tggcgggtcc atcgtgcgcc ggaaagaccg aactggcacg
    44041 gggagtcgcg gaccgtctgc acgcgccgat catctccctc gattcctact acgtcgacct
    44101 ggctcatctg ccgtttgagg agagaccgcg cacgaacttc gacgttccgg agtccctcga
    44161 taaggacctg ctcgtgaacc aactccggca gctttcgaga ggcgaaaaga ttcacgtacc
    44221 ggtctatgac ttcacccgtc acgttcgagc ctcggatacc cgttgcgtgc gagccggcga
    44281 gttcgtcatc gtcgagggcc tgttcgcttt gtactgggag gagctgcggg ccctgctgaa
    44341 aaccacggta ttcgtgtacg cgaaagacga catctgcttc gcgaggcggt tggagagaga
    44401 cgtccgggaa cgaggccgca ctccggaatc ggttcgggag cagtttgact ccacagtccg
    44461 cccgatggcg gagaaataca tcttgccgac acgcctcttc gccgacctgg tgctcagtgg
    44521 gacagacccg ttagagcagt ccgtcgcaac ggtactggat ggcgtcagga tctccgatgc
    44581 acgaggaggc tgagcgcata actacctgcc ccactccggg ggcgtgggcc gaactgggga
    44641 gtaacagggc cgcggtgtca gcttatggtt tctttagcct gggagaggtt gttgttggtc
    44701 acttcccaga tcgtgtaaat gctcttgtgg ctgccaagga aaaacgccga ggctaccagg
    44761 catacgaaag ccaacaggag cgtgtactcc accaagtcct gaccttcctg ctgcccaaga
    44821 aagtccaagc aaagggacct catcgtcagt cttttcctcc tgcgattctg tgccactctt
    44881 gactgagtat gggacggaca cagcccgccc gcgtttggtc cgtgcgagtt cagcctatcg
    44941 taaagccaca gactcccagt tgcaatagta ccttggatag cataggatat caccttccta
    45001 gaacttcgcc ggcaaccgca gccaaaaagt aaagttatga cggagtcata cgaatttcgt
    45061 cgtcctggat gatttgcgag ttgaccggcg tggcgaaacc gccggagact atcggtatgc
    45121 cgacacgtag agggtttctg gccggagcag tcgcgggcac ggtgtctgcc gcatcgcaaa
    45181 ccggaggttc ggccgtctcg ctccggaccc acgaatggtt cggggacaaa ctggaagagt
    45241 tcacgttccc gcccggatgg cgcgtttccg tgcagcacat gaaggggcac gctacgtctc
    45301 tcctggctcc ggcggcaatt cggacggccg tacagaatcc cgttggcaca ctgccactgc
    45361 gggagatcgc cgcaggcaag aagactgtgg cgatcgcatt tgacgacctg acccggccga
    45421 ctccgaccta tgaaatcgtc ccccatgtaa tcgaagagct gcgcgtagcc ggtattcgcg
    45481 acgagaacat cctgttcgtg acggcatatg gcgcccacta ccagatgaac gggctggaag
    45541 tggcaaagaa gctcggcgag gaggccgtcc ggcggcatcc gtggatcaat cacaacatct
    45601 gggagaacct tgcggatctg gggacgacga aagccggcaa ccacctgctg atcaatacct
    45661 actactcaaa agctgacgtc aagatcacac ttagcgggct gaaggcccac gggacgccgg
    45721 gctacggcgg cgggccgaaa ctcatcctgc ccggcatctc ggggatgaag acgatccgtt
    45781 acatgcacac ggagatcaaa cgggcgcgca gaccgcagac gggcccggac ggagtaccga
    45841 tcttccgcgt ctgggagaac gagcagcgcc aggacatgat cgaggcggcg aggcgcattg
    45901 gagtcgattt cagcgtgcag acggtctaca accaggaccg ccgtccggtg gacgtggtcg
    45961 cgggtgacat cgtcaaggcg caccacaagg ctgcgcggta cgccgtaaac cacctcgcca
    46021 cggaatacgc caaggatgct gatattgttg tggtcaatgc ctaccccaaa ggcggccaac
    46081 tacatgaaca cttcggatgg ggctcgcgcg gactgaaaga aggcgggagc atcgtggtga
    46141 tcaaccagaa ccccatgggt gagtttgtct ggcattacct cgatgagtgg aaattcaacg
    46201 aagggaagtc gtatttcgaa cagagggcag cccgaaagcg ccgctttccg caggcccggc
    46261 aggtcttgat ttactcgcaa tatctccagg ctcgggaatt ggaccacccg tatttcccgc
    46321 ccgatgccga aggtgtacgg tcctggagtg acgtcgtcga ccggcttaag cagaagcatc
    46381 gtggcgacgt ccacgttgcg gtctacccct atgccggcat ccagcacggg atcgcgcggc
    46441 tcgacctgcc agaggaaacg taaaagggcg gcctgaagcc atggctccgg ccgcccatgg
    46501 tggatagact ccgacgcggc tacagtggct tggccaccgt aatcgccgtt ccatcgctgt
    46561 cagtctcggg atcgtgattt gcgctggcct gataggtcag gtttgtccca gtgatgttgt
    46621 ttacggtgaa cgtcgcactg gtcttcttgg cagggatacc accggtggtc acgctgcact
    46681 gaccgtttcc gtccgtagtg catgtggctg cgcccgagaa cccgccgctc cacgttccgg
    46741 agaccgtagc accagagacg gtgccgtgag aaccgttgtg aaccgtgacg gtgattctcg
    46801 cttgccactt gttgcgcggc agggggcttg aggcgccgtc aaggtcaccc gaatgcactg
    46861 gagtcgcggg cggcggtgga ggaggcggcg agccgcaagg gctgatctgg aaggatccca
    46921 cacgagtccg ccacgaggca gaactcactc cgttgtaata ctcctgcgtg taccagaagc
    46981 tacagtcgct ttcattaacg gacatcgcgc tgtagtcgcc ccagcggccg ctggagtgtg
    47041 tttgatagcc gctgccggcg acaatcgttc cctcaccgag ggtcatctgg ccgagctggt
    47101 cactcgccaa ccgtccggtg tatcggattg acgggtagac gctggtactg gatgcgctgt
    47161 atccgagggc gacattgccg gaactgtcca tagcgatact acccatccag cgatgggtgc
    47221 tatccggcga gtaggtgccc tgttggtaaa tgttccagcc ggcgcccgta tctcttagct
    47281 cgtaccagcg aattccggcg aggtcggcgc cgttcacgtc caccgtgtgg ttggtgacca
    47341 ttgcctgatg tgtgccgaag ttccgatact gaagacgaaa caacagccgg tccgcgatcg
    47401 cgtccacttt cgtgccaccc ggttgcggga tgcaattcct tgagccattg cacatgttgc
    47461 catcgaaact tgccgtcggc aggatcctgg cgagtgtaaa ggtggatgat gccggctggg
    47521 tccagttggt ctggaacttc cagatctcca gctgatcggc ggggtaaccc caggcgttgt
    47581 cgtcgatctg tgcaaagtag tttggagtcc cggtgggcgg cgccgggcca tcgagatccg
    47641 aaggcagcat cccgccgaga ttcgggtccg tggagtagag atcgaaatat actgcccggg
    47701 cggattgccc gagcaacatc ttgtctcgct cgaaaaccac cgcacccgcg ccgccccaac
    47761 tcagactcgc ttggttgaat tggttcatcg acatgtagta gccatccggc cagaccgcaa
    47821 atttcgggta gtcgttcatc ttattgctgc tgatttggaa cccataccgg taccaggcac
    47881 cggttggatc gggcgtctgg gaaatcgcga tgcactggta gaacggcccg aaggggaagt
    47941 tcggcagggc gaactggctg atgagccagc ggtccgcgag gtgatcgtag aggacgatcg
    48001 ggtcaccatc gttgcgggtc tggcaggggc caccgaatcc gctgaagagc gtgttgatgt
    48061 tcgcagggcc gtaaagcagg gtaccactct tgttgtagat ggcgaaggca aggttgacgg
    48121 cctggacgta atgattcggg ccgatgtccc cgacagtgtc gggaggcaga acaccgctta
    48181 cattgttgac gccctcaaag gtcacggttg ttgtcggtgc tgcctcggtt ccaggagcgc
    48241 tctgcacaac ggtatcggtc tcacccggag gcgtaacacc cggccggttg ggcagccact
    48301 tgcgcggcag ttcgagaatc tcacccgccc tgggcggatt gggcctgatg gtgtgcagcg
    48361 gagcagagga gtcgtgccgg tccgaataga tcacgtgtgc cggcggattc tgaccttccg
    48421 ggggcgccgc ggcaagacta gttgccaaaa aggaagcgaa cagcaagaga agccgtattc
    48481 gcgattgcat tgagtagtct ccagtgggtg cagtcttaag ttcccagtga ggattcagcc
    48541 cacggcgctc gccccacctt tccccaaaat cggagcgtcg cccgggagcc gaccttaggc
    48601 caacgatagc ggagatgcag acgaagatca atagtacttt cgccagcaga acgaagttaa
    48661 ctgccatggc gagaaggtca gagctagtcg ccggaacttg tcaagacccg gttcccgctg
    48721 tacattcaga gcatgtctca tacatccgtc atccgagatg ccctcgccgc cggagatggg
    48781 atcgtccggc tagctccttg ttgggtaccg agatccttcc taatgcccgg cggccgcctg
    48841 aagttggacc cccgcgacct gtatgccttg ggcgcgcatc gcggcggcat cgacgagcgt
    48901 tggttctcat ccacaaccaa tgctgacaac ggtcctagta cccccgaaga cgaaggactg
    48961 agctacatag cctccaacgg caagagggcg ctgctgaagg aagctatcga gacgctaggc
    49021 aacgactttc tcggcgccga cgtcatgcgc gagcagggtg gctggaacct gctgtgcaag
    49081 ttcttcgata acttgggacc aatcccccac catatgcacc aaagggacga acatgcccgc
    49141 cgggtgggcc gcaaaggcaa gccggaggcg tactacttcc ctccccagta caacgtgaag
    49201 gagaataact tcccttacac gttcatggga cttgagccgg gcacgacgaa agacgatgtg
    49261 cgtcggtgcc ttaagcgctg gaacgagggt gacaacggcc tcctgtacca ctcccgggcg
    49321 tacaaactga agcctggcag cgggtggcag atcgatgccg gtattctgca cgcgccggga
    49381 tcactggtga cctacgagcc ccaagtgaat tcggacgtat tcggaatgtt ccagtcgatg
    49441 gtcgaaggtc gcccggtgcc atgggatcta ctcgtcaaag acgttcctcc ggagcatcat
    49501 caggatcttg actacatcat cgacatgctc gattgggagg ccaacgtcga tccggagttc
    49561 gccaaacacc ggctgttcca cccggcaccg gctcgtccca aggatgagat ggctgaatgc
    49621 ggctattccg agcagtggat tgcatactcg acgccgtact actcggcgaa ggaacttacg
    49681 gtcttccccg ggcgctcagt caagattcgg gacgcagcgg ccttcggcgc tatcgtcgtg
    49741 gccgggtggg gagcgatcgg caagatggag gtcgagactc caacactgat ccgctacggc
    49801 cagatgacta aagatgaagt ttttgtaacc gcagccgccg cctgggaagg tgtggttgtc
    49861 accaaccgga gcgacagcga gaacctggtc atgctgaaac acttcggacc gggcaatccc
    49921 gatgcggcgg ggttcatcgc atgaaattcg gggcaaacac cctcatctgg accgcggcct
    49981 tcgaccgtac gtacctgtcg ttgctcccgc gcatcaagga gcgcggattc gatggcgtgg
    50041 agattgcgcg gttcgacttt cacgacttcc cggccgcgga gattcgcagg aacctggaac
    50101 agaatggcct ggagtgcacc ttctgctcgg ctctgacggg agacctgagc ttgatcagcg
    50161 aggacgcagc ggtccggaag aaagcggcag actttctcca gtacggaatc cgcaccgccg
    50221 cggagctcgg agcgaggatt ttcatggggc ccttctactc tcaggtcggc tacctcgccg
    50281 gccgccggcg gacggcggat gaatggacgc gggcggtgga gggtcttcaa tcgttaggcg
    50341 agtcgctagc ggcaaacggc gtcgtaatca cgatcgagcc gctgaaccgc tttgagacct
    50401 attttctcaa cacagccgcg gacgccgcgg cgctgtgcga cgaggtcgat catccgaacg
    50461 tcggcattgc tctggacacg ttccacggga atatcgaaga gaaggatctc ggatctgcgt
    50521 tccggaccgc cggcaagcac ctgaaacacg tccatgcctg cgagaacgac cgcggaattc
    50581 cggggagtgg tcacgtggac tggagagggg tttttggagc gctgcacgaa ctccgatatg
    50641 acggctggct ggtgatcgaa agctttggtt tcaccattaa ggagattgca gcggcagcgt
    50701 gtatctggcg agatctggcg ccctctcccg aggacattgc gttcgagggg ttgaagtttc
    50761 tgaagaatcg cgccgcgtga aaagcgaaag gggtccggaa cgggacccct tgccgcgtcg
    50821 aacagagttg accctagagt tgtaccttcc tggtcccctc ctgatcgggg cgattctgat
    50881 gttaccacga aaccaaagca gagacgtaat tcttcgatga gctcaggatc ggatgccggc
    50941 gccgcgaacc agcaggctct cgacctgcga ggcaggcagg gacgcactga gcagatatcc
    51001 ctgcactttg tcgcacccga ccgctcgcag cgcttccatc tggttctcgc tctccactcc
    51061 ctcggcgacg acgtccagtc ccaagccatg cgccagtgat acgatcgcct gaaccagggg
    51121 cattgtattg gagtcgacgt cgagttcctt gacgaacgac cggtctattt tgatcgtgtc
    51181 gatcggcagg cgccgcaagt agctgagcga tgagtagcca gtgccaaaat cgtcgatcga
    51241 gatactggcg ccgagcgccc gaagccgctc catctggcgg ctcgattcct caaaatcccg
    51301 catgacgaca ctctcggtga gttccaactc caggaatgac ggatcgagac cgctgtgcga
    51361 gaggacctga gccacggttt cgacgaaatc cgcgcgggca aattggacgg gtgacacgtt
    51421 aacggcgacc ttcaccggct catagcccgc ctgccgccag gcgacggttt ggcggcacgc
    51481 ctcttccagc acccacgccc cgatctcgac aatcaggccg ctttcctcag ccaccgggat
    51541 gaactgggag ggcgatatga cgccgagctt cgggtggttc caaaccaata gcgcctccag
    51601 cgccgcaagc cgtccttcga tcgccagttg cggttggtag tacaactgca actcgccatt
    51661 cgccatcgcc cggcggaggg cattctccaa ttccagttgc tctagagccg cggcgcccag
    51721 atccggtgta aagaattggt agatgttctt tccttgattc ttggcgcggt acattgcgtt
    51781 gtccgcattc cggagcaacg tgactgagtc acgcccatcc ctggggaaga cgctaatgcc
    51841 aatgctggcg gtcacgaaca gctcgaatgc atccaccttg aacggggtct tcagagcatc
    51901 aagtagtttc tgcgcgaccc gcgtagtgtc ggaagggtct cgaagctcgg tcaaaatcaa
    51961 ctggaactcg tcaccgccca tccggcccag gctgtcggtg cgcctgagac aactctcaaa
    52021 gcgtctcgat acctgctgca acagggcgtc gccaatggcg tgtccgaggg tatcgttgat
    52081 ctgtttgaag cgatcgaggt cgatgaacag caccgcaacg tgccagccat gacgcgtagc
    52141 ctggtcgagc gcctgatgaa gccggtcctc aaacaaaaac ctgttgggca gacctgtaag
    52201 ggcatcgtgc atggcctggt aggccagcct gtcgttcagt tgacgctgtt cgacggctac
    52261 cgctgccaag cggccggcca tgtccacgag ttcgattaca gcgggagagg cttcgtgctg
    52321 ctcgcgaagg tagatcataa ataccccgag cgccctgcca tcgcccgaca ggatggggga
    52381 acaccagcaa gagcgcaagc cgagattccg aagcagtgcg gcctctcgcc ggaaaagcgg
    52441 atcggcagcc agattcggga tcaggagcgg tttgcccagg agtgccgtct cgcacggtcc
    52501 gagggcggtc gtcacatcaa gcccgtcaaa ggcgtcggca atcggctgtg gaaggctcgg
    52561 cgcggccccg gggtgcagac gtccgtcgcg caccagcagg accgaacaga ggagatcgga
    52621 acactggttc tcaaccagat tcgccaacct cgcgagtacg gtttccaaag gaccgttgct
    52681 ggctacaagt tcaagaacct ggttgcggtc ccgctccagt atctcagccc gctttcgatc
    52741 ggtcgtctct cgcaatacgg aaacccaata ggtgaaggcg ccacgctcgt cgacgatcgg
    52801 aacgatgttc aggtcgaccc agaactcgga gccatcctta cggtagtgaa tcagttcaac
    52861 ccgggccgtt tcccagcggc ggacggcggc acatatctga tccagaaccg cctcgtcggt
    52921 tttcgggccg tgaagtattc ggggagtcct gcccgcggac tccacatccg agtagccggt
    52981 aatccgggag aacgcatcgt tgacgtagac gatgcgcgag ccgagggggt cgtccgggtc
    53041 cgcctcagcc accatgacgc catcgttgga attcacgacc acggatttca agagccgcaa
    53101 ttgcgcctcg gctctttttc gctccgtgat gtcgcgagcg atcccctgga cgccgaccgg
    53161 gatgcctttg cggaagagca ggcgggtgct cacttccaga atgatccgcc ggttgtcttt
    53221 cgtgacgatg gtgacctcat aggtcgtttg cggcgcacca ccgagcttct gctggatcat
    53281 ctcgatggta aggcgctgag actccgggtc aagaacctga aaaatgttca tcttcagcgc
    53341 atcttcacgg ctatagccgg tgatgcgctg ggctgccttg ttggctgacg tgaaattgcc
    53401 gtgaaggtcg tgggtataga cgacgtcgct tgcgttctcg aacaattcgc gatagcggtc
    53461 atcgccgaac ccggggtttt ccgcggcggc gatggtcttg gcgaccgagg cccgaagctc
    53521 cccttccgcc atggccatac cggcaagatc acgaagcgcc gcgatctgag gcggatcgag
    53581 ttcgcgcgtc gtggtgtcca tgacgcaaag agtcccgatg ttcgctccgg tgggagtccg
    53641 caaggggacc ccggcgtaaa accggacctt gtgtgacccg gtcacgagcg gacaggtgcg
    53701 gaaacggtcg tccccaaggg catcgggcac caccatcacc tgatcggaca gaattgtgtg
    53761 ggtgcagaag gaagaagctc gcggaatctc gcggataccg agcccgtgtg aagccttgat
    53821 gtgctggctg tcgcggtcta tgacactgat agcggtgaac ggcacaccga acaggcgggc
    53881 taccagccca gccaggcggt cgaaaacagc accggagaga acgtccggaa cgacatagct
    53941 ctgtaactcc gctagcctct gttcctcgtt ctcgggtaac tgaccgtcca tcgaaacacc
    54001 cgaaccgcaa cggtgatcac cagcggaccg gacaccggct cctacgtcct ctactataca
    54061 agcgcagttg agtgagattc cacccggcgc ggccgtgctg ggttaggatt tcagtcgtct
    54121 cgcatcaggc gggaggtctt ccgcagccag ccgcgcaaca tggcagtgaa gtcacgcgct
    54181 tgggggtcgc tccgaaattc ttcgacggtg aacttcactt tgcggcgcca gttcgggtat
    54241 tgccaagtgc tgccaggcaa gttttgctgg tctgtctcct tcgtcagatc ctccagggcg
    54301 agcaacatca gcattgacgg cgtggacgcg agaaatccaa cgaccgcgtt gtggagttct
    54361 cccgagagct ccggcacgtc cgcgcagttt ctggggaacc attccggaag gtctccacat
    54421 cgcaaaagca cgtccagcaa attctgcttt tcgcgagccc gcactccgag ctgtctttga
    54481 tacgcatcac cgttgccgag cagaccggcg acccgccgtg cctcgatgtc tctacccaac
    54541 cagaacccgg ttagcgtcgg gaggtcatgt gtcgtgatcg atgccaaaga ctggcgggga
    54601 tactcgtggg gagttcggaa ttcgtcctgc tcattcttct caaaatagag cagccggtag
    54661 ctcagtatcc cgaagcggcc gagttcttcg cgaatcttgg gagccaccgt gcccaagtcc
    54721 tctccgacca ccagaacctg gtttcttaca ctttccagcg cgaggatgcg caacaggtct
    54781 ccatgtttct ccagcacgta cgtgccgtcg gcggcttgcc ccccgtccgg gatccaaaaa
    54841 aggcggaaga accgcatcac gtggtcgatg cgtagcgcgc cgccgtgact gcagttccgg
    54901 cgaatcgact ctacgaagaa ccggtaaccg gtctcgctgt ggtattcggc atttggcggc
    54961 gggaaagccc aatcctggcc ctccggcgcg aacgcatccg gcggcgaacc gacccggcag
    55021 ccggacacgt aacagcggcg gtgtgcccag aagtccgacc cgaagcgatc cgtagccagc
    55081 gcgagatcgt gatagagacc gatagagagc ccttgccggc gcgcgtgttc ctgggcctcc
    55141 tgaagttgca agtccgccag ccactgaagg tacttgtgaa agagcaccag ggaggcgttt
    55201 tccgcagcga agcgccgggt cgcttccgac tcaggatcct ggtactcgga gggccactcg
    55261 gcccacaccc acgtgtcggg gttgcgggcg tgaatccact cgtccagggc gcaaaaagtg
    55321 gcataacgat ccagaaggtc gctctcacgt tcgcagtatc gtcgaaacgc ctcggcgcgg
    55381 acggagtggg cggcggaact tcgggaaaat tcgcggaatg cgagccgcag aaagcgaagt
    55441 ttcaaagcat agaccctctc gtactcgacg aacggcgccg ttcgaagggc gcagatctcg
    55501 gcttgtactt ccggcgaggg gaaagctgcg accgcctcgg cggaggtccg aaagtcctcg
    55561 acccgctcga cgtccaggta gatagggttc ttgtagtaaa tgcagacggg gagataaggg
    55621 cttgtattgt atggctggcg gttgtgaatg gcgtggagag ggtttagcgc cacgaactcc
    55681 acaccggcgt cctccgccgc ccaatccacg atgcgttcga ggtcagagaa atctccgcat
    55741 ccccagttcc gctccgaccg cagtccgtag agattgaccg ctactcccgc ggcgcgcctg
    55801 cccgaagcca gactggacgg aagccagacc cgatcaggcg tcacaatcaa acgcatcgtg
    55861 gcgcaaaccg ggccatggtc tccgcggact tgaacacgta cgtcgtggta gccaagagga
    55921 atgcggagcg gcaccaacgc tcgaaccttc cggaagcggc ggccccggag ctcggccgcc
    55981 tccacggagg gcaggccatc cacgcgaaga tcgaggcttt gatgagtacc atcctccaga
    56041 cggaattcca ccgcgaccgg ccagccggcc gcttcgtcag ggatcgccag cgggagttcc
    56101 accatgggtg cgttttcgct gaccacgaga acagcaggca aaggccgcga ccaggagcac
    56161 cacagtcgtt cctccatcgc gcgatcgaga cactcctgcg tatcgcaggg cactcccaac
    56221 gctcggagga tcgcggcaat cacgtccggc cgggtgacga cacgatttcc gaatacgtcc
    56281 cagtactcgc gctcgatccc gaacagagaa gcggcgcgat gaagcgcctc ttcgtgactc
    56341 ttcgacggca caaacgacaa cgttggcttg gacatgaatg tgatcagatg gttgcggacg
    56401 gccagccgcc ggcaattgtt ctaaacattt tatccgaacc gagccgtctc ctgcgctgaa
    56461 tggacatgag acaatagagg catgggattg gacgcctcgg cccgcttcaa catgactggg
    56521 gtttcggacg tgcccgagaa cttcttcgcg cgcgtcgatg aagagagcct cgtagcgctc
    56581 tcggacgggg tcgtaaccac cacgttgggt caagcgatca actgggctcg caagaactcg
    56641 atttggccga tgacatttgg actggcctgc tgtgcgatcg agatgatgtc gatgagcgct
    56701 tcccgcttcg atatcgcccg attcggggcc gaagttttcc ggggatctcc gcggcaatcc
    56761 gatctgatga tcatcgcggg gcgggtatcg aacaaaatgg cgcctgtgat ccggcagctc
    56821 tatcaacaga tgcccgagcc gaagtgggtg atctcgatgg gagcctgcgc tacctcgacc
    56881 ggggtcttta gcaattacgc gcttattccg gtgaatcaga tcattccagt ggacgtgtac
    56941 gtgcctggct gtccacctag gcccgagcag ctcatttacg gcatcatgct gttgcagaag
    57001 aagatgcagc aagaagcggg caccttgaag gatgttctta acttaggctg aaccggcgga
    57061 caggtgcagc cgcggacctg ccggagtcag gtataatgag gttgggtgtc gggcaccaaa
    57121 agtgggcgaa agcccacttt tttgttggac acgaagcacg agacatgcac gaaacggtga
    57181 tcgataaggt ccgggagatc gcccggcgcg tcggagactc ggaagggatc gagatcgtcg
    57241 atgtcgagat ggcgggggcc ggcaacggcc gcaggctgag gatcttcatt gacaaaccat
    57301 ccggcgttac gcatgccgat tgcgaatcga tttcgcagca ggtcggcgcc gtactggacg
    57361 tcgaggatgt ggtgcccggg gggcgttata cgctggaagt cagttcgccg ggggtggaac
    57421 ggaagcttac cggcgcaagg gactttgagc ggttccaggg caagaaggcg aagattgtgc
    57481 ttcgtgagcc ggttgaaaac cggaaggttt gggaggggac attggcgggc gtcgccgatg
    57541 gcgttgtcac gatcgagcct gctccgggga agcaggtccg cattggcctc cagcaggtcc
    57601 agagggcgaa tctgaagttc gagtggtaat ctcctgacat tcaggaagaa gcagaggttt
    57661 cgttttggga acgatctacc agtctattga gattctgagc aaggagaagg gcatcgatcc
    57721 gcagatcgtg ctggacgcgg tcaaagacgc gatgcttgct gcggcgcgga agcatttccg
    57781 gaccgaggag gaactcgttg cggatttcga tcagcgggcg gggtcgattc agatcttcgc
    57841 cgtcttcaag gtggtcgaaa acgttgagga tccggtaaag gaagtcactg tggagcaggc
    57901 gcgccggatt gatccagccg ccgaactggg tacggaagtg cgggtttcca aaccgaccga
    57961 cctgctgggc cggatctccg cccagaccgc caagcaggtc atcctgcaaa aggtgcggga
    58021 ggcggagcgc gagacgatct accaggagta ctcaggacgg gtcggcgaac tggtcaattg
    58081 cacggtaaag cgggttgagg gactggatct cgtggtggac ctcggtaaga ccgaagctcg
    58141 cttaccgaag aaagaacagt ctaagctgga gagcttcagc gtgggggagc gcttccgctg
    58201 cgtgatccgg agcgtcgaaa aggcagggaa gaacgccggc ctgattgtct cccgggcggc
    58261 gccagagttg gtgatgcggc tcttcgaaca ggaagtcccg gaaatctacg acaacacggt
    58321 tgtgatcaag ggctgcgcac gggaagcagg tgaacgcacc aagatcgccg tctggagccg
    58381 tgaccgggac gtcgattgcg ttggggcctg cgtcgggatg aagggcatgc gagtgcagtc
    58441 gatcatccgc gaactgcgcg gcgagaaaat cgacatcatc gaatactccg acgatccggt
    58501 ggtattcgcc actcacgctc tcagcccggc gaagatatcg agggtcacgg tgatcgaggc
    58561 tggcgataaa cacatggaag tgattgtcga cgattcccag ttatcgctgg ccattgggaa
    58621 aaagggccag aacgtccgcc tgtgcgccaa actgctgggc tggcggatcg acatcaagag
    58681 cgaagaggag aagcgtcagg aagttgagtc gcaaatggcg gctctggtcg tccctggcgc
    58741 cccggtctca gttcttatcg accagggcct cacggagaag accgtcgaaa agttgcttga
    58801 ggcaggggtg tcgaccgtcg aaaagctcgg gtccatgacg ccggaggaac tcgaagagat
    58861 ccctggcatc gggcccaaga tggtggaaaa gatccaggtc gccgtcaaca actactacgg
    58921 acaatacgaa gaggcgatgg cgggatcacc ggaagctgtt gcggcggtga cggcggcgat
    58981 tgaatcgatg ccgcccggcg aagtccccga gggacaagca gagccggcgg ccgaagcgat
    59041 acccgctggg agcgaacagt ccgatgcagt tgcgacgcag ggggattcgc agatgcctga
    59101 tgcagccgcg tcggaggggg agtcaactga cagcagggtt tccgcacccg aaggtgagtc
    59161 caaagatgag tcgaaaccac gggaaaatgg gtcgggtgac ggggaatctg atacgataaa
    59221 gaacgtggag tagtactgca ttagtagtgt gcccggttgc aggtggcttg tcggagtacc
    59281 gcgaaggggg tttttccatg ggcaggtttt gtgtaaacgg acgctgcccg cgagatttgt
    59341 tttatgagta agatccgaat caacgagttg gcgcgggagc tggaagtaaa gcccaacaag
    59401 ctgcttgatc tgctccctca gttcgggatc acggagaaga agacgcattc cagctcactc
    59461 gaggacgata tcgccaacaa gttgcgcagg tacttcggcc tgctcgtaga ggaaccggtc
    59521 gaggcagaac ccgagattac cccaagggag atggcgccgg aaaccggccc gtccgtagaa
    59581 gccatcgagc aaaccacagc tcgcacggat accccgacgg aaacacaacc aacgacggtt
    59641 gcgcccgttg agagcctggc gcccaccgct gtcccgccgc cggacgccgg acctctcaaa
    59701 gaatcgccgt tcgcaccgag aacggcgccc caaagattgc gtccgccgct tgcgggtagc
    59761 ggccattccg ctcagccggc tgcggttgcg ccggatgcaa ttccgacgcc ccccgcgccc
    59821 ccaacgccgg tggtagcgag cggacccggc cggccggcag tcgtggcggc gaaaccgctg
    59881 ccggtggctc cccgtccggg gcagatcttg accgggccgc ggcagccctt tcccgtaaaa
    59941 ccggagattg taaagtcatc cgttccgtcc gccgagatgc ccgccgagcc acaggcgccc
    60001 ctgccccaag cggcaactgt cactcacacg ccgcggtccc aggccaaggc gactctggca
    60061 ggacagccgg ctgcgcgtcc cgtcgtgcca ccgcgccccg atctggtggc tcggcttgcc
    60121 caaacccaga tcaaagcggt tccaggacaa ccgcagccgc gaccgggtcg ccctgcgacg
    60181 gcgccggttc cgggccagcc gatctatcgg ggacccatcc ggccgggcca ggcgctgatg
    60241 accagaccat caccgggagc cccaggcagg gtggccagag gccgcccgct tcatccgact
    60301 tcagcgaggc tggaaccgcc gccacccgta ccaacagaac agcgccgccc gggtgaccgc
    60361 cgtgctcgta ctccggcgga acgggagcgg gagcgcgaac gcgaagaaag aacacttcgt
    60421 ccgtcgcgac gcgacgtgcc ggtcgttctg ccttcattga accggcagat caccgtcgcc
    60481 gaaggcatca cggtaaaaga gctgtctgaa aagcttgggc tgaaggcaaa cttggtgatc
    60541 aagaaactgg tggagcggaa aatcttcgcg accatcaatc agaccctgga ccttcaactg
    60601 gccgaggaac ttgtgaaggc gttcggtgcg acctcaacac ggcttagcta cgaggaggag
    60661 gcggtgcagg aggtcgggca ggccgaggcg gagcaggatc tgctgaagcg gccgccggtg
    60721 gtgacggtga tggggcacgt cgatcacggc aagacctcgt tgttggatgc catccggctg
    60781 accaacgtgg ccgagcgaga ggcgggaggc atcacccagc acatcggcgc ctaccacatc
    60841 gagaagaacg gccgaaagat cgttttcatc gatactccag gccatgaagc tttcacgcgg
    60901 atgcgcgccc gcggcgccaa ggtaaccgac attgtcgtgc tggttgtcgc cgccgatgac
    60961 ggcgtgatgc cgcaaactct cgaggcgatc gaccatgcac gggctgccgg cgtaccgatc
    61021 atcgtcgcaa tcaacaagat cgacagaccg gacgcgcaac ccgagagaat caaacaacaa
    61081 ctcgctgacc gtgggctcct ggcagaggag tggggcggcg agacggttgt cgtgcccgtg
    61141 tcggcaaaga caaagcagaa cctcgatttg ctgctggaaa tgatccttct ggtggccgac
    61201 atacaggatc tgaaggccaa tcctgcccgg ccggccgtgg gttcggtact ggaagcgcaa
    61261 ctggatcgcg gacgcggtcc ggtggcgacc gtcctagtcc ggaacggcac gctttcggtg
    61321 ggtgactatt tcatttgcgg gtcagtcttc ggcaaggtcc gtgcgatgtt cgacgaccgc
    61381 ggcgagccgg tgaagtcagc ggagccctcg atgccggtgg aggtgatcgg cctcgagtcc
    61441 ctgcccgagg tcggcgacac gttccagatg gtcacggaca ctgccaaggc caaacagatt
    61501 gtaatctacc gggaggcaaa ggcccgcgaa caggcgatgg cgcggtccag ccgtgtcact
    61561 ctcgaccaac tccacgagca gatcagggcg ggagaggtga aggaacttcc gatcattctg
    61621 aagacggatg tcggtggaac cgcagaggtc ctcaccgaca cgctgcaaaa gctctccacc
    61681 gacaaagtgc gagtccgggt gttgcacgcc agcgttggcg cgatcaccga aaccgacgtg
    61741 ctgctggctt ccgcctcgaa tgccgttatc atcggcttca atgtccggcc ggagcgaaac
    61801 gccgcgcagt tggctgaaaa cgagaaagtc gacatacggc ttcacaccat catttacgac
    61861 cttgtagacg agatgaagag ggccatgacc ggcatgctgg agcccgtctt ccgcgaggtc
    61921 ttccgggggc gcgcggaggt caaggaagtc ttccgcatta cgaaggtcgg tattgtggcc
    61981 ggctgccaag ttatcgacgg gacggtcact cgggacagcg aagtccgggt gcttcgcgat
    62041 aatgtggtcg ttcacaccgg ccgagtcggt tccctgcggc gattcaagaa cgacgtcagt
    62101 gaggtcaagt ccgggatgga gtgtggcgtg tcgcttgaaa acttcggcga cgtcaagcct
    62161 ggcgacgtaa ttgaagcctt cgtaaccgag caggtggcag cagaagcatt ggcatagacc
    62221 ggggacagga actagcggaa cgtatcacca tggtccccac tccgggcgcc caatatggcg
    62281 acgatcggtg ttctcaccct cgagatgagg cttgaacacg cccattccct gaaagataag
    62341 cgtcatgtag tgcaggggtt gaaggaccgc ctccggcaca ggtacaacgt tgcggtcgcc
    62401 gaaatcgatt gccaggacct gtggcagagg gcggtcgtgg ccgccgtcac cgtctcgggc
    62461 gagcacgcgc atgcagagcg ggtgctgcaa tcggtcgagc gggaggcgtc gtctctgctc
    62521 ggggccgctc ttgtgggggc gacggtcgag tggctggagt gaagcggtat ggatgagcac
    62581 cgaacgcagc gtgtctccga agcgatccgg gaagaattgg ctgagatcat cggctacgag
    62641 atggccgatc cgcgattagg cggtatcgat atcaccgagg tcctgatcac cccagacatg
    62701 cgccgtgccc agattcgggt ccatctcgga ggcggggaac aggcacgcca gcaagcgatg
    62761 acagcactgg acggcgcccg ggggttcctg cgccggaagc ttgccgcccg gctccggctg
    62821 tttcgcgtcc cggaccttcg cttcgaggcg gatgcctttg tatcggacag ccggcgcctt
    62881 gagaagctgc tcaggcgtac gaggagaggc cgcccgcaac cggaggtttc ggacgaaaaa
    62941 gaaccataaa aatgaaacat tttgtggtac ttttgttatt gtttcccgcg cttctttgcg
    63001 gggaggagtt acttcggctc gaggacaccg ccgatgcgat gggggccacc tacacggtgg
    63061 ttctgtacgg cgatgaccag gagcagttac agtccgcgtc tcaggcggcg tttgacgaag
    63121 tgcgaaggtt ggaccggatg ctgtccaact accgccctca aagtgaatgg agtgaggtga
    63181 accggagcgc cgccgaacgg ccggtccggg tgagcccgga gttgttcgag ttgctggagg
    63241 cgtgcctttc ctatagccgc gcgagtgagg gggcgtttga cgtcagcgtc ggtcccctga
    63301 tgcgggtttg gggcttctat aagggcacag ggcggctgcc aatgaaggag gaaatcgcag
    63361 cggtgctccc cgtcgtgggg tacagaaaca tcgagctcga ccgggagaac cagacggtcc
    63421 actttcggcg ccgcggcgtc gaactggatc cgggtgggat cggcaaaggt tatgccgtgg
    63481 atcgtatggt cgcccgcatc agggagttca agatcacgtc ggcactcgtg tccgccggcg
    63541 gaagcacgat ttatggcctc gggacaccac ccggcgacaa gggctggaag gtgaaaatcc
    63601 gccacccccg tgaatggaac gaaacgattg aagaggccta cctcaaagac gagtccatgt
    63661 ccacgtcggg agactatgag aagtttttcg aggcggaggg caaagtttac agccacatca
    63721 tggatccacg gagcggatac cccgccccgg ggatgctgtc tgtctcggta atcgcgaaga
    63781 agtcactcga tagcgaggcc tggacgaaac cattcttcat tctcggccgc ggatgggcgg
    63841 ccaaacataa acctcaggga cttcgcgcgt tcacatgcga agacagatcg gaactggcat
    63901 gcgcgtggct tcaatgaaca gccgctttct cgatgcttgc cgccgccggc cgacggacgt
    63961 gcgcccggtg tggttcatgc ggcaggcagg tcgatacatg aaacaatacc gggatatccg
    64021 agcgaaatac gggatcctgg atatctgcaa gagaccggac cttgcagcca ccgtcaccct
    64081 tcagccggtt gagattcttg acgtggacgc cgctatcatt tttgccgatc tacttctgcc
    64141 agttgaaccc atggggatga agctgcggtt tgcggcgggt gaggggccgg taatcgaaaa
    64201 ccctgtccgg acctcgcagg acgtggatac gctctccatc tccaataccg acgaactcgg
    64261 ctacgtcggc gaggcaatcc agactgtcgt caagtcgcta ggtggcaagg tgccagtggt
    64321 cggcttcgtc ggcgcgccat tcacgctcgc cagctacgcg atcgaaggcg gcgcctcgcg
    64381 gacttttctg cgaaccaagc agatgatgta ctccaatgag acgctatggc ggcggctgat
    64441 gggaaaactg gtggacgttc ttgggccgtt cgccctcatg caagtggcct ctggcgcgcg
    64501 atccatccag gttttcgaca gttgggtcgg cgcgcttggc ccggacgact atgtccgctt
    64561 cgtggcgccc tattcccggg cgctgatcga gcgcattcgc tccagcggcg ttccggtgat
    64621 tcacttcggg acgggcgcgg cgggcttttt ccgcgaattg cacgcggccg gcggcgacgt
    64681 gatgggagtc gactggcgca tcaacatcga ccaggcatgg atggacatca gttaccgctc
    64741 ggcgattcag ggcaacctcg acccggcggc gctgttcgca ccgctgcccg agctcaaggc
    64801 aaaggtccac gaactgctga aacgcaccgg gacccgccca ggacacatct ttaacctcgg
    64861 tcatggcatt ttgcctgaga cgccggta
//
LOCUS       DATIFQ010000037         4741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1926446, whole genome shotgun sequence.
ACCESSION   DATIFQ010000037 DATIFQ010000000
VERSION     DATIFQ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926446"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VLE22_01710"
     CDS             <1..418
                     /locus_tag="VLE22_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB13142.1"
                     /translation="FIMAVQVYGCNHVVIEVTDARKAVKFYSDVFGLKMLRGGEGAAW
                     CKLGEHQFMAIFEVEKLQPDRVKHFGLMVRDAQQIKEVRKKLTKKYKLKLHPGFRCDF
                     RDPWGNRIQVGDLSDESLVWLLPYQEVQKAGITFGR"
     gene            423..869
                     /locus_tag="VLE22_01715"
     CDS             423..869
                     /locus_tag="VLE22_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB13143.1"
                     /translation="MKAHYLGHVVFYVKDLERSLSFYRDLLGFQEVGKIFNGAAAALT
                     SGRTHHELLLIQVGDAPGPPQGRRRGLYHIGIKVGDSLDELRAAKKELERAGVTIDGM
                     SDHTVSQSLYLRDPDGNEVELYVDADESLWKSNPAAVVSPIKPLHI"
     gene            1139..1375
                     /locus_tag="VLE22_01720"
     CDS             1139..1375
                     /locus_tag="VLE22_01720"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HSB13144.1"
                     /translation="MEKPIIAAKQPAVLTLDPGTYHWCQCGRSKNQPFCDGSHKGTGI
                     TPVEFSLTEKKQVALCQCKQTKNPPFCDGTHKTL"
     gene            1683..2753
                     /locus_tag="VLE22_01725"
     CDS             1683..2753
                     /locus_tag="VLE22_01725"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HSB13145.1"
                     /translation="MIKVNKTWLVRIGFVAVAAGLVFLAWHYFQPKQLGKGFASGNGR
                     IEAVEIDVAAKTPGRIKDILVNEGDFVTAGQIVARMDTEVLESQRREAEAQLRQAQSA
                     VERAHSLVAQREAEKSAALAVVAQRESELRLAQQLLKRSEPLVAEQAIALQEVDDARA
                     TVRGAQAAVSAAKAQVAAADAAIATARSQVLEANSVVEAARATIERLQADINDSELRT
                     PRDGRVQHRVAQPSEVLSAGGKVLNLLDLADVYMTFFLPTAAAGKLAIGSEVRLVLDA
                     VPQYVIPAKVSFVADAAQFTPKTVETASEREKLMFRVKAQIDPALLRKHIHQVKTGLP
                     GMAYVRLDPNVEWPSTLQVKLP"
     gene            2753..>4741
                     /gene="rbbA"
                     /locus_tag="VLE22_01730"
     CDS             2753..>4741
                     /gene="rbbA"
                     /locus_tag="VLE22_01730"
                     /inference="COORDINATES: protein motif:HMM:NF033858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HSB13146.1"
                     /translation="MPHDQPATMAMAAEETVVRISNVSLRYGKRLALDAISLDLPAGK
                     MVGLIGPDGVGKSSLFALISGARAIQEGRIQVLGGDMAEAQHRRAVCPRIAYMPQGLG
                     KNLYPTLSVFENIDFFGRLFGHHETERARRIEDLLDSTGLRPFRDRPAGKLSGGMKQK
                     LGLCCALIHDPDLLILDEPTTGVDPLSRRQFWDLVARIRAGRPGMSVLMSTAYMEEAA
                     RFDWLVAMDAGRVLATGTPQELLARSGTTSLEEAFIALLPEEERRGHRAVVIPPRTGD
                     DGDIAIEAKDLTMRFGDFVAVDHVTFRIERGEIFGFLGSNGCGKTTTMKMLTGLLPAS
                     EGKAWLFSQAVDPNNLATRQRVGYMSQSFSLYSELTVRQNLALHARLFHVPAEEISGR
                     VEAVAARFDLADVIDALPEALPLGHRQRLSLAVAMIHRPEMLILDEPTSGVDPIARDA
                     FWQIMIDLARRDKVTIFISTHFMNEAERCDRISLMHAGRVLVTGSPAALTEQRGANTL
                     DDAFVSYLEEVVAQDKMKETPLAADMGPSQAPAFSALPAPAARAWEKRRGFDLRRLFS
                     YTRVEALGLRRDPIRATLALLGSVLLMFVVGYGINMDVENLSFAVLDRDQSSTSRDYV
                     LNLAGSRYFIERAPIFDYAELDRRMRSGELSLALEIPPG"
BASE COUNT          934 a         1428 c         1500 g          879 t
ORIGIN      
        1 cttcattatg gcggtccaag tctatggctg taaccatgta gtgatcgagg tcacggatgc
       61 cagaaaggcc gtgaaattct attcagatgt gtttggctta aagatgctcc gcggcggcga
      121 aggagccgcc tggtgcaagc tcggcgagca tcaattcatg gcgatcttcg aagtcgagaa
      181 actgcagccg gatcgtgtga aacatttcgg cttgatggtc cgtgacgcgc agcagatcaa
      241 agaagtcaga aagaaactga cgaagaagta caagctgaag ctgcaccccg gcttccgctg
      301 tgattttcgc gatccctggg gcaaccggat tcaggtcggc gatctgtccg acgagtcgct
      361 cgtctggctg ctcccctacc aggaagtcca gaaggccggt atcacgttcg ggaggtgaac
      421 ccatgaaagc gcactatctc ggccacgtcg tgttctatgt gaaggatctg gaacggtcgc
      481 tgtcgttcta tcgggatctg ctgggattcc aagaggtcgg gaagattttc aacggcgcag
      541 cggcggccct cacgtccggc cgcacgcatc atgagctgct cctgatccag gtcggcgatg
      601 cgcccggtcc gccgcaaggc cgcaggcgcg ggctctatca tatcgggatc aaggtcggcg
      661 atagcctcga tgaattgcgc gcggcgaaga aggaactgga acgagccggc gtgacgatcg
      721 acggcatgag cgaccacacc gtgagccaga gtctgtatct ccgcgatccg gacggcaacg
      781 aagtggaact ctacgtcgat gctgacgagt cgctgtggaa gagcaatccg gcggcagtcg
      841 tgtcgccgat caagccgctg cacatctagc aaactcttaa aagtccatgg gggattgtag
      901 attgtgctcc ggaacggaat cttttggagc tctaccgctt gatagtcatc tgtcccttcc
      961 tgccatggtt ccagcggaag actgtggcct ttctccacaa gccacccgca ctatcacctc
     1021 tcttaagagc gaaatatagc gctttgtaca tggcaagagt ttcgcacatt cgtacgttcg
     1081 ccaagaagca gaggacgtga gccaacagca acgatgtcaa cgaagaggag agcagctcat
     1141 ggaaaaacca atcattgcag ctaagcagcc ggcggtgctc acactggacc cgggcaccta
     1201 ccattggtgt caatgcggac gctcgaagaa tcaaccgttt tgcgacgggt cgcataaagg
     1261 aaccgggatc acgccggtcg agttttcgct gacggaaaag aaacaagtcg cgctctgcca
     1321 gtgcaaacag accaaaaacc cgccgttttg cgacgggacg cacaagacgc tctgaaggtc
     1381 ttgagagagc ggaaaagtaa agatcgcact catcggcgcc accggatatg tcgggtcggt
     1441 catttgacag aaagcgttga atcgtgggaa ttcttcgata tcagttgttg ctcaacgggc
     1501 agcctcgtct cgccccggtg cggtgcccgc tcaccggagt gtgtcgtcaa cgtgcagaag
     1561 atggagcgga tgatcttctg gatcgatatc cggttcagcc tcggggacgt cggcgcaccg
     1621 ttcgcctcgt gtgacaagcc catacgtcac tgccgacgcg gcatcagtca ccggataccg
     1681 gaatgatcaa ggtgaacaaa acatggctcg tgaggatcgg attcgttgcg gtggcggcgg
     1741 gtctcgtctt tcttgcctgg cactatttcc aaccaaaaca actgggtaag ggctttgcca
     1801 gcggcaacgg ccgtatcgag gcggtggaga tcgacgtcgc cgccaagact ccgggccgca
     1861 tcaaggacat tctcgtgaat gagggtgact ttgtcaccgc cgggcagatc gtcgcgcgga
     1921 tggacaccga ggtgctcgaa tcccaacgcc gggaggccga ggcccagttg cgccaggcgc
     1981 agagtgcggt ggaacgggcc cacagtctgg tggcgcagcg cgaggccgag aaatctgcgg
     2041 ccctggctgt ggtggcccag cgagaatccg agctaaggct ggcgcaacag cttctgaagc
     2101 gctccgaacc gctagtagcc gagcaggcaa ttgccctcca ggaagtggac gacgctcgcg
     2161 ccactgtccg tggcgcgcag gcggcggtca gcgcggccaa ggcgcaggtg gcggcggcag
     2221 acgccgcgat cgcgacggcc cgatcccagg tgctcgaagc taattccgtg gttgaagcgg
     2281 cgcgggcgac gatcgaacga ctgcaggcgg acatcaacga cagcgaactg agaacacccc
     2341 gggacggccg tgtgcagcat cgcgtcgccc agcccagcga ggtgctcagt gccgggggca
     2401 aggtgcttaa cctcctcgat ctggccgatg tctacatgac cttttttctt cccacggctg
     2461 ctgccgggaa gctcgcgatc ggatccgagg tgcgcctggt gctcgatgct gtcccccagt
     2521 acgtgatccc tgcgaaggtg tccttcgtgg ccgacgccgc tcagttcacg ccgaagaccg
     2581 tggagaccgc cagcgagcga gagaagctca tgttccgggt gaaggcgcag atcgaccctg
     2641 cgcttctcag gaaacatatc catcaggtca agaccggctt gccggggatg gcctatgtgc
     2701 gtcttgatcc gaacgtcgag tggcccagca ccctgcaagt gaagttgccg taatgcccca
     2761 cgatcagccg gccaccatgg cgatggcagc ggaggagacg gtcgtacgta tctccaacgt
     2821 cagcctgcgc tacgggaagc ggctcgccct ggacgcgatc agcctcgatc tgccggcggg
     2881 gaaaatggtc ggactgatcg gacctgacgg cgtggggaaa tcgagcctgt tcgcattgat
     2941 ctcgggggcc cgcgcgatcc aggaagggcg gatccaagtg ctcggcgggg acatggccga
     3001 ggcgcaacat cgccgcgcag tctgtccgcg gatcgcttac atgccgcagg gtctggggaa
     3061 gaatctctat ccgacgctgt cggtgttcga gaatatcgac ttctttggcc gcctcttcgg
     3121 ccaccacgag accgagcggg cgcgccgcat cgaggacctc ctcgacagca ccggcctgag
     3181 gccgttccgc gatcggccgg ccggaaagct ttccggtggc atgaaacaaa aactcgggct
     3241 ctgctgcgcg ctcatacacg atccggatct gttgattctc gacgagccga ccaccggagt
     3301 cgatcctctg tcgcgccggc aattctggga tctggtcgcg cgcatccgcg ccggccggcc
     3361 cggcatgagc gtgctcatgt cgaccgctta tatggaggag gccgcgcggt tcgactggct
     3421 ggtggcaatg gacgccggcc gcgtgctggc caccggcact ccacaagaac tgctcgcgcg
     3481 cagcggcacc acctccctcg aagaagcctt tatcgcgctg ctcccggagg aagagcgacg
     3541 cggccatcgg gcagtggtga tcccgccccg cacaggcgac gacggcgata ttgcgatcga
     3601 ggcgaaggac ctgacgatgc gcttcggcga cttcgtcgca gtcgatcatg tcacctttcg
     3661 catcgagcgc ggcgaaatct tcggttttct cggctcgaat ggctgcggca agaccacgac
     3721 catgaagatg ctgaccggcc tgctgccggc cagcgagggc aaggcctggc tgttcagtca
     3781 ggcggtggac ccgaacaacc tcgcaaccag gcagcgcgtc ggctatatgt cgcaatcctt
     3841 ctcgctctac tcagagctga cggtgcgcca gaatctggcg ctgcacgcca gactgttcca
     3901 tgtgcccgca gaggagatca gcggccgggt cgaggcagtg gctgcacgct tcgatctggc
     3961 cgatgtgatt gacgcgctac ccgaggcctt gccgctgggc caccgccagc gcctgtccct
     4021 ggcggtggcg atgatccatc ggccggagat gttgatcctc gatgaaccca cctccggcgt
     4081 cgacccgatc gcccgcgatg ccttctggca gatcatgatt gacctcgccc gtcgggacaa
     4141 agtgaccatt ttcatctcca cccatttcat gaacgaggcc gagcgctgcg accgtatctc
     4201 gctgatgcat gcgggccggg tgctcgtcac cggttcacct gccgcgctga cggagcagcg
     4261 cggggcgaac accttggatg atgcgttcgt cagctatctg gaggaggttg tggcgcagga
     4321 caagatgaag gagacgccgt tggccgcgga catgggaccg tctcaggccc ctgcgttttc
     4381 cgctcttccg gcacccgctg cgagggcctg ggagaagagg cggggcttcg atcttcgccg
     4441 cctgttcagc tacacccgtg tcgaggcgct cggactgcgc cgtgatccga tccgcgccac
     4501 gctggcgctg ctcggcagcg tcctgttgat gttcgtcgtg ggctacggca tcaacatgga
     4561 tgtcgagaac ctgtcctttg ccgtgctcga ccgggaccag agcagcacca gccgcgacta
     4621 tgtgctgaac ctcgccggct cccgctattt catcgagcga gcgcccatct tcgactatgc
     4681 cgagctggac cggcgcatgc gcagcgggga gctgagcctc gcgctcgaga ttccccccgg
     4741 t
//
LOCUS       DATIFQ010000038         3581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2692844, whole genome shotgun sequence.
ACCESSION   DATIFQ010000038 DATIFQ010000000
VERSION     DATIFQ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2692844"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..602)
                     /locus_tag="VLE22_01735"
     CDS             complement(207..602)
                     /locus_tag="VLE22_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13147.1"
                     /translation="MTPAADLLNPDSNSALYAATVLNLYVDLPDTPLRASIQDQRLAQ
                     RLFEAHVPLSLVEAALLLASLRRLCRPSELPPLPRVRSLAYFQPVIEELQANPVPKGY
                     LGYLRLKLRSIVDKADPAQVQKTTFSEDR"
     gene            complement(599..1384)
                     /gene="istB"
                     /locus_tag="VLE22_01740"
     CDS             complement(599..1384)
                     /gene="istB"
                     /locus_tag="VLE22_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HSB13148.1"
                     /translation="MSSPANMTTSPRNSLAGQLQQIGLRALPAELDDFLARATKARWS
                     PRMLLEQLAQAEIADRSRRSLERRLRFSGIKSFKPMADFEWTWPTKIEREVIERALTL
                     DFLPEGRNLVLVGRNGLGKTMIAQNICHAAVLAGSSVFFRSAPALLEDLHRQAPEGRH
                     RRLRSYANVGLLCIDEVGYLSFDDKAADLLYEVINRRYERKSLIVTTNRPFKEWNEVF
                     PNATCIATLLDRLLHHADVTVIEGDSYRVRESEQETAARRRKK"
     gene            complement(1344..2840)
                     /gene="istA"
                     /locus_tag="VLE22_01745"
     CDS             complement(1344..2840)
                     /gene="istA"
                     /locus_tag="VLE22_01745"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HSB13149.1"
                     /translation="MISPETRAEIRRYFFAEHWKIGTIAHELSVHPDAVRNAIESDRF
                     RRGPALHASILDPYLEFVRQTLDQHPRLRATRLYQMIRDRGYTGSIIQLRRTVARLRP
                     PAREAFLRLQTFPADQAQVDWAHFGQVAVGRARRKLSCFVMTLSYSRALYLEFFFDQT
                     MENFLRGHVHAFQDWGGQPRVILYDNLRSAVLERRSDQIHFHPRLMELCAHYHFVARP
                     CQVRAGNQKGRVERAIRYVRDSFWAGRPFTTLAESNRQALLWRDQIAHQRPWPGDDSR
                     TVEQVFAEERPRLLPHPLHPFSTDLIVGVRSGKTIYVRFDLNDYSIPPEAVGRSLTLV
                     ASDTLVRILDGTAEIARHARSYDRHQLVLNPAHAQALRQAKRKAFDATPGGRLALAVP
                     ESEKLLDQAFAQGESAGSQTAQLLKLLDLYGSAALRRAVSEALERNTPRASSVAFLLR
                     KQQRSAKSTAPMAVDLSRHPEAQSIDVRPHDLETYDELARKHDDKPKE"
BASE COUNT          695 a         1025 c         1172 g          689 t
ORIGIN      
        1 cgataagcgc ccaacggccc atataacaga tatcttgaac ctcttatcta gtttcgtcaa
       61 gccttcgtaa aaaggggaaa tggggttgct tcgcgattgt tgccgagatc ggtaatctct
      121 gtgtgatcaa cagtagatta atccgaacgc tggagatcag ctatgcctcc tggccatctc
      181 gaaacgcgca ctcatgccgc cgtctgttag cgatcctctg aaaacgtcgt tttttggact
      241 tgagccggat cggccttgtc cacgatgctt cggagcttga ggcgcaggta tccgaggtag
      301 cccttgggga ccgggttcgc ctgaagctcc tcaatgacgg gctgaaagta ggcaagcgat
      361 cttactcgtg gtaggggcgg cagttccgag ggccggcaga ggcggcgcag ggaagccaac
      421 agcagtgcgg cttccaccaa ggaaagggga acatgggcct cgaacaggcg ttgcgcgagt
      481 cgttggtcct ggatgctggc gcgtaacggg gtatccggga gatccacgta aaggttgagc
      541 accgtggcgg catagagtgc ggaattggag tccgggttca acaggtccgc ggcgggagtc
      601 atttcttcct ccgccgggcc gcggtttcct gttcactctc ccgaacccgg taactgtcgc
      661 cttcgatgac ggtcacgtcg gcatggtgca gcaaccgatc cagcagcgtg gcgatacagg
      721 tggcattcgg aaagacttcg ttccattcct tgaatggccg gttggtcgtt acaatcaggc
      781 ttttccgttc atagcggcgg ttgataactt cgtagagtaa gtcggcggct ttgtcgtcga
      841 atgaaagata gccaacctca tcgatgcaca acaaacccac gttggcatag ctgcgcagtc
      901 tacggtgccg cccttccggg gcttgccggt gcaagtcctc cagcagcgcc ggggcggagc
      961 ggaagaagac ggaactgccg gccaacacgg cggcgtggca gatattttgc gcaatcatgg
     1021 ttttgcccaa cccgttgcgg cccaccagga ccaggtttcg gccctccggc aggaagtcca
     1081 gagtgagggc gcgctcgatg acttcgcgtt cgattttggt gggccacgtc cactcgaaat
     1141 cggccatggg tttgaagctc ttgatgccgg aaaagcggag ccgccgttcc aggctgcggc
     1201 gggaacggtc cgcgatttcg gcttgcgcca actgctccag cagcatacgc ggcgaccagc
     1261 gtgctttggt ggcccgcgcc agaaagtcat ccagctccgc cggcagggcg cgcaagccaa
     1321 tctgctggag ttgccccgcc agactattcc ttgggcttgt cgtcatgttt gcgggcgagc
     1381 tcatcgtagg tctccaaatc gtgggggcga acgtcgatgg attgggcttc ggggtgacgg
     1441 ctcagatcga ccgccatcgg cgcggtggac ttggcggaac gctgctgctt gcgcagcagg
     1501 aaagccaccg aagaagcgcg cggcgtattc cgctccagag cttccgagac ggcacgccgc
     1561 aacgcggctg acccgtacag gtccagcagt ttgaggagtt gcgccgtttg actgccggcc
     1621 gattctccct gcgcgaacgc ttgatccagc agcttttcac tctccggcac cgccagcgcc
     1681 agacggccgc ccggcgtggc atcgaaggct ttgcgcttgg cctggcgcaa agcctgtgca
     1741 tgagccgggt tcaacaccag ttgatgccga tcataggagc gtgcatgacg agcgatttcc
     1801 gcggtgccgt ccagaatccg caccaatgtg tcggaggcca ccagggtcag cgagcgcccc
     1861 accgcttccg gcgggatgga gtaatcgttg agatcgaagc ggacgtagat ggttttgccc
     1921 gatcgcaccc cgacaatcag atccgtgctg aagggatgca gcgggtgagg cagcaagcgc
     1981 gggcgttctt cggcgaagac ctgctcgacc gtgcggctgt catctcccgg ccaggggcgt
     2041 tgatgtgcga tctgatcgcg ccagagcagt gcctgccggt tgctttcggc cagcgtggtg
     2101 aaggggcggc ccgcccagaa ggaatcgcgc acatagcgaa tggctcgttc cacgcggcct
     2161 ttttgatttc cggcgcggac ctggcagggg cgcgcgacaa agtgataatg cgcgcacaac
     2221 tccatcagcc ggggatggaa atgaatctgg tcgctgcggc gttccagcac ggcactgcga
     2281 agattgtcgt agagaatcac ccgaggctgc ccgccccaat cttggaaggc atgcacgtgg
     2341 cctcgcagaa agttctccat ggtctgatcg aagaagaact ccagatacaa tgcccgtgag
     2401 taggacagcg tcatcacgaa gcaggacaac ttgcgtcgcg cgcggcctac ggcgacctga
     2461 ccgaagtggg cccagtcgac ttgggcctgg tcggcgggaa aggtttgcag gcgcaggaag
     2521 gcttcgcgtg ccggcggacg cagtcgggcc acggtacgcc ggagttggat gatgctgccg
     2581 gtataaccgc ggtcgcggat catctggtac aggcgcgtgg cccgcaagcg gggatgttgg
     2641 tccagggtct ggcggacgaa ctccaggtat ggatcgagga tagaggcgtg gagcgccgga
     2701 ccgcggcgga agcgatccga ttcaatggcg ttacgcacgg catcggggtg aacgctcaac
     2761 tcgtgggcaa tggtgccgat tttccaatgc tcggcaaaaa agtaacggcg aatctcagcg
     2821 cgggtttcgg gactgatcat gggtttctac caccgggatc gtggaatgcg tgggaacggg
     2881 tccacccagt agccagggcg gccgcaaacg acgcagcaca gcatcgggac cgaggctttg
     2941 gggtaccact ctgaggtcga tccagctctc gatgttgcct gcgcgttccc acatggcaat
     3001 actgccggag aaaggaccga agcggaagat tgatccgtca cgcgcgcccg gcaccggcgg
     3061 cgccggtact cctgctgccg gtcccgatga tcctgtcggc cttcgggact ctgctgatgc
     3121 cggcggttgg cgcgccgtcg ttgttggagc cgggcggcgt cgcggcactg gggactgcaa
     3181 taacgctgtc cgcggtcgca gtgttcacaa atgcagaaca cggcgtaaca cccctcgccg
     3241 cgacaaaccc gttgacgcag aaccacttca ccgtgaaccg ccatgccgtc gcagggcggg
     3301 gggctggaca acggcagcca atcatgtcag cttagaacca gcttgctgcg gtgacccgtt
     3361 gagggttgcc aagccgggac cctgccggtg caacggtggg gtcccattcc cgcctcgggc
     3421 atgaacttta tcatttcggc aagcttctgg gaatgatgat ggggacggat gatccgcgcc
     3481 ttcggcgcga tgatattgct cgcacttcgg ctaaagttca aaaaacgacg atttcagatg
     3541 atcgatgaca gcttcgaggt gtcggagcgg acagtggctc g
//
LOCUS       DATIFQ010000039         3467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2693533, whole genome shotgun sequence.
ACCESSION   DATIFQ010000039 DATIFQ010000000
VERSION     DATIFQ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2693533"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..621
                     /locus_tag="VLE22_01750"
     CDS             193..621
                     /locus_tag="VLE22_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13150.1"
                     /translation="MNTGAAAQRIEQSVDGPAWFQYCFSVYVRAGVSTPVVLYSTDGT
                     VSADTRATAGPLWQRVVHSVRLESPTEATAFGFELGPGAAVELYGFQAECQPAPSAYR
                     RTTTRSGVYERSRFGDDVLTLRADGPGEYSCAVRIVAGMW"
     gene            771..>3467
                     /locus_tag="VLE22_01755"
     CDS             771..>3467
                     /locus_tag="VLE22_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HSB13151.1"
                     /translation="MKELSITETPLFLVECRTVSGAIERWSTHRVAVDGVLYEPRILR
                     HNLFEIRAEADDAIDGVSRVSITLGNADSHCSQIERGGGWKGARVTVWFLFFDLKHGQ
                     PASEKAVIFRGVANPPDEITESTMRLTVSNRMNLQRLLLPEVRIQKRCPWKFPSTVEQ
                     RAGAATGGSQGKYSPFFRCGYSADVAGGEGNLNGGSAYSSCNYTRASCAQRGMFDVDT
                     AGHTTRRFGGIEFVPSTTLVRSYGEKGHHVSPVAENEGRYNDFVPLVYGTAWYAPPVV
                     FARNDGNLTHLEVLLGMGEIEGALKVLVNDIEIPLGRQGADMTATGWYSIVSQGSRTG
                     AFNPDFGDQNGSAAGDPYGSMAVLSLVTPNRINDGRSLPRVQVLLQGLRLATYSTAGE
                     YLGEEFSNNPAWVILDILRRSGWSLEEIDTRSFARTAEFCAQMIDGADLYGGPLQMPR
                     FQSNLVVRKRRSAAELIRGIRNGSRLYLTLGMDGRLQLNAENNIALQQPVKPQGSNSS
                     EPLNGGWPCYEFGDGTSGFPGILRRENGEPAIRLWTRSTADTPNRFSVEFQDALNEYQ
                     QDSLSLVDVDDALRAGQEISVPLQALGIPNFHQAARAIQIQLDKSVRGNTYVEFETSV
                     RGVQLKPGDIIALTYLKEGFQRQPFRIRSISPGDNFGTAVIVAQIHDDALYTDEVGGA
                     TGAGGRRQPGFEVGIPRPLIGSVLDAEGEPQFEITERADETSDGQMKVWLSAGFVAPS
                     RPSPGGGNIPMLSLSPDVFDTGGALAGDQTLYYAVSALGAEGDESPLSFLARAVIPPG
                     TNTNRVRLTGLRLGPETASFRVYRGSNPSHLFRIAGGQPPAAEFTDPGFEKQLATAPD
                     ENYDHANFYWRFELLPEIAATLHSAETIGNGTLQMAENEYRGM"
BASE COUNT          711 a          965 c         1217 g          574 t
ORIGIN      
        1 atggggcgca cgaactcgtt gagtggcgga taagcctgac cggcttgagt gaggcagagc
       61 gaaacgccat cgagtccctg tttcagtcga cggaaggacg ccttggagag tttacttttc
      121 tcgatcctac ggacaacctg cctcgcagcg ggcttttcgg atccagccgg taccgcgagg
      181 gcgacgcggg cggtgaacac aggcgctgcg gcacagagga tcgagcagag tgtggatggg
      241 ccggcgtggt ttcagtactg cttcagcgtc tacgtgcggg ccggcgtctc gacgccggtg
      301 gttctgtact cgacggacgg gacggtgtcc gcagacacgc gtgcgacggc gggtccgttg
      361 tggcagaggg tggttcactc agtgcggctg gagagcccca ccgaggcgac ggcattcggg
      421 ttcgaactcg gaccaggggc agcggtggaa ctatacggat ttcaggccga gtgccaaccg
      481 gcgccgtccg cataccgccg gacgacgacg cgcagcggcg tctacgagcg ctcccggttc
      541 ggcgacgatg tgctgacgct gagggccgat gggccggggg agtactcgtg cgcggtgcga
      601 atcgtggcgg ggatgtggtg aaggggaaag gggaagagag agcgaggacg ggagaacggg
      661 aacaaggaaa atggggacag acagccattt cgcaacaaca gcgaaatggt ggtcagtccc
      721 ccattttccg acggcgaaat tggggtcagt ctgtggcgac gatatccgaa ttgaaagaac
      781 tgagcatcac cgagacgccg ctgtttctgg tcgagtgccg gacggtgtcc ggagcgatcg
      841 aacggtggag cactcaccgt gtggcggtgg acggcgtgct ctatgagcca cgcattctcc
      901 ggcataacct gttcgagatc cgcgccgaag ccgacgatgc aatcgacggg gtttcccggg
      961 tgtcgatcac gctgggcaac gcggattcgc actgctcgca gatcgagcga ggcggcggat
     1021 ggaagggggc ccgggtcacc gtctggttcc tgtttttcga cctcaagcac ggccagcctg
     1081 cgtcggagaa ggcagtgatc ttccgcggcg tggcgaaccc accggacgag atcaccgaga
     1141 gcacgatgcg gctgacggta tcgaaccgga tgaacctcca gcggctgttg ctgcctgagg
     1201 tacggattca aaagaggtgc ccgtggaaat tcccatcgac ggtcgagcaa agggcagggg
     1261 cggcgaccgg cggctcccag ggaaaatact cgcccttctt ccgctgcgga tactccgccg
     1321 acgtggcggg cggtgagggt aacctcaacg gcggcagcgc ttattcatcc tgcaactata
     1381 caagggcgag ctgcgcgcag cgaggaatgt tcgacgtgga cacggccgga cacacgacgc
     1441 gaaggttcgg aggcatcgaa ttcgtgcctt cgacgacgtt ggtccggagt tacggggaga
     1501 aggggcatca cgtttcaccg gtcgcggaga acgagggacg ctacaacgat ttcgtaccgc
     1561 tcgtgtatgg caccgcgtgg tacgcgccgc cggtggtgtt cgcacggaac gacggcaacc
     1621 tgacgcacct cgaggtgctt ctcgggatgg gcgagatcga gggagcgctg aaggtactgg
     1681 tcaacgacat cgagatcccg cttggacggc agggcgcgga tatgacggcc acgggctggt
     1741 attcgattgt cagtcagggg agccgtaccg gcgccttcaa cccggacttc ggggatcaga
     1801 acggaagcgc ggccggggat ccatacggga gtatggcggt actgtcgctg gtgacgccga
     1861 accgaatcaa cgacggacgg tcgctgccac gcgttcaggt gttgctgcaa ggtttgcggc
     1921 tggcgacgta tagcacggcg ggagagtatc taggggagga gttcagcaat aacccggctt
     1981 gggtgattct ggacatcctg cgacggagcg ggtggagcct ggaagagatt gacaccagaa
     2041 gcttcgcgcg gaccgccgaa ttctgcgcac agatgatcga tggcgcggac ctgtacggag
     2101 gcccgctcca gatgccacga tttcagtcga atctcgttgt gcggaagcgg cgcagcgccg
     2161 ccgaactgat ccgaggaatc cgcaacggaa gccggctgta cctgacgctg ggaatggacg
     2221 ggcgtttgca gctcaatgcg gagaacaaca tcgcgctcca gcagccggtt aagccgcagg
     2281 gcagcaacag tagcgaaccc ctgaacggcg gatggccctg ctatgaattc ggagacggca
     2341 catcgggttt cccagggatt ctgcggagag aaaacgggga accggcgatc cggttatgga
     2401 cacgcagcac ggctgacact cccaaccgat tcagcgttga gttccaggat gctctcaacg
     2461 agtaccagca agacagcctg tcgctagtgg atgtggacga cgctctgagg gcggggcagg
     2521 agatcagtgt tccgctccag gcgctcggaa taccaaactt ccaccaggcg gcaagagcca
     2581 ttcagattca actcgataag tccgtacgag gaaatacgta cgtcgaattc gagaccagcg
     2641 tccgcggagt gcaattgaag ccgggcgaca tcatcgcgct gacgtacctc aaagagggat
     2701 tccagcggca gcccttccgg atcaggagca tctcgccggg ggacaacttc ggtacggcgg
     2761 tcatcgtggc gcagattcac gacgatgcgt tgtacaccga cgaagtcgga ggcgctacgg
     2821 gcgccggagg gagacggcag ccgggctttg aggtcggaat cccgcgtccg ctgattggga
     2881 gcgtcctcga cgctgaggga gaaccgcagt tcgagatcac agagcgcgcc gacgaaacct
     2941 ccgatgggca aatgaaggtg tggctatcgg ccgggtttgt ggctccgtca cggccaagcc
     3001 ccgggggcgg aaacattccg atgctgagcc tctccccgga cgtgttcgac acaggcggag
     3061 cgctggcggg cgaccagacg ctctattacg cggtgagcgc gctcggggcg gagggagacg
     3121 agagcccgct ctcgtttctg gcgagggcgg tgattccacc ggggacgaat acgaatcgcg
     3181 tgcgcctgac ggggctgcga ttggggcccg agacggcaag cttccgcgta tatcgcggat
     3241 cgaacccgtc acacttgttc cggatcgcag gcggccagcc gccggcggcg gagttcaccg
     3301 atccgggctt cgagaagcaa ctggcaacag cgccggacga aaactacgac cacgcaaact
     3361 tctattggcg gttcgagctt ctgccggaga tcgcggcgac gctccactcg gcggaaacga
     3421 tcggcaacgg cacgctccag atggcggaga acgagtaccg cgggatg
//
LOCUS       DATIFQ010000040        11450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1353249, whole genome shotgun sequence.
ACCESSION   DATIFQ010000040 DATIFQ010000000
VERSION     DATIFQ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353249"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VLE22_01760"
     CDS             <1..1215
                     /locus_tag="VLE22_01760"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HSB13152.1"
                     /translation="VTFTDFNFTAPGYQEHAAEWMASKSIEYANETTLMCYNAEGWRN
                     HLEKQWDRLLAEFDFDGLYIDHWINIRYCWNSRHGCGGYLGSFATDGYHDFARRARRV
                     VARHTDGQGVMLLNANMLLFSGMVPWFDIRLNGENDDPLKMREETIVTTWNGWGQGVQ
                     SLAMWRPQQDTRSMINLMTTFAIPFRLGRGAMPKDVEEWRTSEATETALAREFWGIWR
                     FFGVNGAEKFSSFDVGGELEMTQPGSIVNGFARDGRVLAVMGVQGGKGVREEKLRILS
                     PSQLGLKDGLRYRIVDLRNNRYAGPARSATELAEVPVQLTNDDPLVLLIEPEHKGPRL
                     VWFRGADEVTAGPKFEFRVKAVPGSPLELYVDEAEQQIRPVTPGFDRKVAGDFAVFSG
                     TVPDDGVVKLTQ"
     gene            1392..2699
                     /locus_tag="VLE22_01765"
     CDS             1392..2699
                     /locus_tag="VLE22_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB13153.1"
                     /translation="MTVSRREFTSAALGGGLASLPLTLSCVRSASSGPASPGSASPAL
                     NSRYGKLDDVLKQPVLKKQFFSAPVIIETVELLRLNNSFLCRVRSRDGAEGISVGHSG
                     MRTLFPIFLHNLQPFFLGKDARELDRILERVYIYGFNFRYNGIALGLPLATIEFAILD
                     MLGRIAKKPVGELIGEIHNPEVAVYVATEWREKPVEESIELIKKAVAEYPEARALKIK
                     VGGLMFMTTDMDAVGPPGRTERMIPLVRKTFGDDMALYADSNSFYSVPEAIRVGKLLE
                     EHKYRYFEEPVMFDHIEEIKQVADALAIPVANGEQDNSFYGFRWLIANDGIDIVQPDN
                     YYFGGLIRSMKVARIAAAFGKSITPHMSGGGLGYVYNIQFVSAVPNAGEHHEFKGLQT
                     NVRFECKTSPLKVVGGKIKVPTGPGFGVDIDPEWVKKHQIVKL"
     gene            2776..3777
                     /locus_tag="VLE22_01770"
     CDS             2776..3777
                     /locus_tag="VLE22_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB13154.1"
                     /translation="MMTRRDALALPATLLTAHAAPALSRMGIAATCFSAQRPKDTLEF
                     LEFCRSLGAGGVQAPLSSTSPEHLAGLRRRAEQHGMYIEVMAGLPRGEDAETFERTLI
                     AAGEVGARCARVACLGGRRYEMFSRLDEWKQFAAGSRKAIARTLPLAEKHRMPVAIEN
                     HKDWTLEELLALLKEYSSEYIGVCLDTGNNIALLDEPMEFVERLSPYALSTHLKDMAV
                     EEYSEGFLLAEVPFGEGMLDMPRIAEIIRRARPGTKMTLEMITRDPLRIPCLTEKYWA
                     TFADTSPQRLPAMLRTVRAQAAKRIPRPGTVPPEIQANREADNVAACLRYARERMGL"
     gene            3834..5147
                     /locus_tag="VLE22_01775"
     CDS             3834..5147
                     /locus_tag="VLE22_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB13155.1"
                     /translation="MNRRRWLFAAGSAVTAGRVRGSNERVNVGLIGCGGRGRFVAGLM
                     KKTPKVEFTAVADVYLANAERAREWAGPEAKAYQDFRRLLEHKELDAVLVATPDHWHA
                     LATIFACHAGKHVYVEKPLAHNVREGRAMVDAAHRANRLVQAGTQQRSAEHFREVSEI
                     IRSGQLGAVHFVRIWNYFNLSPDGIGRAADGEPPADLDWDMYCGAAPKVPYNPLRHLG
                     TYRYFWDYSGGMITDYGTHRFDTVHQIMGVDSPRTVSASGGRFALRDAGEVPDVLQVT
                     YEYAGFVLSYEMCMISGHGLGGRSAGMRYYNAHGPLDRPHGIAFYGTNGALFCDRIGY
                     EIFPDLKKGSSTDFRMERRAKNTVDATPLHAANFIDAIRGLKPLASPVDIGHCSTTVP
                     HLGNIAFKTGRKLVWNAQKEEIENDREASRLLSRQARAPWQLPVL"
     gene            complement(5221..6819)
                     /locus_tag="VLE22_01780"
     CDS             complement(5221..6819)
                     /locus_tag="VLE22_01780"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HSB13156.1"
                     /translation="MPQSSYRRAVIAPLFAILLSVSYRSSGVDRLEGTILDDETGQPV
                     AAALLLSDGEGKPIEIEGPHSHVQYLGKRRCYVNGSFVLNSRPNRLVVEVRRGIETLP
                     IETAMDLTQHGSQPLTFRLRRWIDMRKEGYVSGDTHVHFLSQSEAHSQMRAEDLNVLN
                     LLVSDFTNDREKFTGDLDPVSTPGNVVYVGQESRDWQNGHVILLGIKRIVEPFAPYGG
                     KFQDRNEPNLVMSRILRDARRQGGTTTWAHFCNLPGAESPIAIVHGLVDAIDLITYDD
                     PTQLPSHWGPWTNSGMSQAEFTVMRSMDLYYQYLNSGFQVPIGAGTDKMGDYIPVGSN
                     RLYARPVGEADYKGWLAGLKAGNGFVTNGPMLTFEVDGRSSGDTVSFKGSHRVRVRAT
                     AKSILPFQSLEIVVNGETAAVKSLPNQYRPTSDGVYSLELEGTIDLDRSSWLAARVAE
                     SPGSRNRILPRGLTVFAHTNPIYFLRDGVKVRERSSVHYLQKYVKGTIHWLNTGARFR
                     NPAEKEEALRLAEEARQAYARLEK"
     gene            7172..7585
                     /locus_tag="VLE22_01785"
     CDS             7172..7585
                     /locus_tag="VLE22_01785"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSB13157.1"
                     /translation="MGYTGDEPTQLLSSDAGPSPAETLLHGMANCVSVTSSYHAAARG
                     LPLDAFKVDFEGDIDLQGFADLDDNVTPAYQNVRGKIHVKAGGSREAIAKFLKFATSH
                     SPMCDSVSRPVHTTFSLVHNGQAISQPVGSAAAAG"
     gene            complement(7607..9190)
                     /locus_tag="VLE22_01790"
     CDS             complement(7607..9190)
                     /locus_tag="VLE22_01790"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HSB13158.1"
                     /translation="MFLCHVAFILAALLQPTSVGKYEVSEISLKTDEMFSNPFTEASA
                     TAVFRSPSGRRVGVPGFYFGGTTWMVRFAPDEAGQWSWQAKLSGKRGAVNRSGVFRCV
                     PSDRRGFVRISKRNPYRFEFEDGTPFYPIGVQTCGYFRVGFDGPNSDGSWKTVPAEMW
                     SKEFQGTTNLIRWQLGAGTKAGCALTLIPVGGQPDRYDTEIAGKMDELLAVQRAHGMS
                     HIMIPFQDMSLWGKDVTAFGTVDDVVNYKSLKAANLPLQEAYLRYIVARFGAFVDIWE
                     LFNEDSYAPNDYLARLAVVIREADPYKHPITTNYTRSSEKWNEIVTWHGYMSISATET
                     DAWLADKIGRLKSYGKPVLNTEFGNKGVLSNVDPVKWRIAAWTAFMNESNILYWGMSG
                     RKTEPGQPRGNSNAYLGPDTRRQLRVLNEFIRDLPLDLRPVECTYSQQNDLRIFALGN
                     EKTTVLYVHHFADHSRPYKLDQRIYVHTGAGRFRVTRMNPEDGRVIRSGSAVTQQQYL
                     FFEMPPLTIDLACRVEREE"
     gene            complement(9248..10654)
                     /locus_tag="VLE22_01795"
     CDS             complement(9248..10654)
                     /locus_tag="VLE22_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSB13159.1"
                     /translation="MNLTQLFDLSFQGRREAVALEFGGRTYTFGEIDDRSNRMAALFG
                     ERGLEAGDRLCVYLSNCLELIDIYLGCVKSGVIFVPINILYREREILHILTDAEPKAV
                     IAAHEMPGSVPFWPVSGLQTPERAERPEIPLDGDAPAAIVYTSGTTGASKGAILTHNN
                     FAANALNLITCWGITAGDRFLLALPLFHVHALGNGLHCWLIGGCRMRLLERFEHQKAS
                     AEFLDFRPTLFFGVPTVYVRLLDLAPETARQIGAFMRLFVSGSAPLPAQVLEEFREKF
                     GHTILERYGMTETLMNISNPYAGERRAGTVGLPLPGVSARIVEGELQLRGPNVFAGYW
                     RREEATRAAFDAGWFKTGDMAERSTDGYYTLLGRKSDLIISGGFNIYPREIEEFLLEQ
                     EEVAEAAVVGVPDRLRGEVPVAYVVPRRPFDAAILEARCRESLASFKIPRAFISVEKL
                     PRTALGKIQKHMLPKWGE"
     gene            complement(10632..>11450)
                     /locus_tag="VLE22_01800"
     CDS             complement(10632..>11450)
                     /locus_tag="VLE22_01800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB13160.1"
                     /translation="GARYIVSVFSGKAAHVAQGFYGGSVVLGSGFVIFAVPQLLGAFG
                     WRGAFLSSTLVAVAVLVWWVIAAPRPEHIVRPAGKFGEMVRSPELWLLGLVQMASFGL
                     VIVVGAWITVLLKADFQMPIKPAGLMGSLVLLLGIGSRPAGGWLLRRMRMTALVRGAL
                     LMNAVACAALAWGQSLALTFIAILALGAGCGLPYAGVFNRAAALFPGRAGAAMGLVNM
                     IGILMILVGAPAVGYLADWTGEFRSSFLALGGFSLLAVAATFGFEKTHEPDTTV"
BASE COUNT         2336 a         3450 c         3475 g         2189 t
ORIGIN      
        1 gtgaccttca ccgacttcaa tttcacagcg cccggctacc aggaacatgc agctgagtgg
       61 atggcttcaa agagcatcga atacgccaac gagacgacgc tcatgtgcta taacgccgag
      121 ggctggagga accatctcga gaagcagtgg gaccgattgc tcgccgagtt cgactttgac
      181 ggtctctaca ttgatcactg gatcaacatc cggtactgct ggaactcccg gcacggctgc
      241 ggaggttatc tcggcagctt cgcgaccgac ggctatcacg acttcgccag gcgtgcgcgc
      301 cgggtggttg cacgccatac ggacggccag ggtgtcatgt tgctcaatgc caacatgttg
      361 ctgttttcgg ggatggttcc ctggttcgac atccgattga acggtgagaa cgacgatccg
      421 ctaaagatgc gtgaggagac gatcgtgacc acctggaacg gctggggtca gggcgttcag
      481 tcgctcgcga tgtggcggcc gcagcaggac acgcggtcga tgatcaacct gatgacgacg
      541 ttcgcgatcc cgttccgcct cgggcgcggc gccatgccca aggatgtgga ggagtggagg
      601 acttcggagg ctacggaaac cgcgctcgcg cgggaattct ggggcatctg gcgattcttc
      661 ggcgtgaacg gagcagaaaa attctccagc ttcgacgtgg gaggtgagct tgaaatgacg
      721 cagccgggct cgatcgtgaa cggcttcgcg cgcgacggcc gcgtgttggc cgtcatgggc
      781 gtgcaaggcg ggaagggagt tcgggaggaa aagctccgca tcctgtcgcc ctcccagttg
      841 ggtctgaagg acggtctgcg gtaccgcatc gttgacctgc ggaacaatcg ttacgccggt
      901 ccggcccgga gcgccacgga gctggcggag gttccggtac aactgaccaa cgacgatccg
      961 ctggttctat tgatcgagcc agaacacaaa ggcccgaggc tggtctggtt ccgcggcgcc
     1021 gacgaagtca ccgcgggtcc caaattcgaa ttcagggtga aagcagtgcc tggttcgcct
     1081 cttgaactgt atgtggatga agcggagcag cagattcgac cggtcacgcc cggtttcgac
     1141 cggaaggtgg ccggcgactt cgccgtgttt tcgggaacgg tgcccgacga cggggtcgtc
     1201 aagttgacac agtagctgtc ccttggcttg atggagcgtc tggttgccag attcgggcgg
     1261 aggcaggagt ataattccgc tgaggccaag catcgaaaac ggaaatttgt attgtcatgc
     1321 aaatctgatg gccggcacta tccttgagtg attgaaggca ccgtaacgat tgaaaccagg
     1381 agatccaacc aatgacagtt agtcgtcgtg aattcacctc agccgccctt ggcggaggct
     1441 tggcttccct accattaacc ttgtcctgcg tccgctcggc aagctctggg cccgcgtcgc
     1501 ccggttcggc ttccccggca ctgaattccc gctatggcaa actcgacgac gtactgaagc
     1561 aacccgtgct gaagaagcag ttcttttccg cgccggtcat catcgaaacg gtcgaattgc
     1621 ttcgtctgaa caacagcttc ctgtgcaggg tgcgttctcg tgacggagcc gaaggcatct
     1681 ccgtcggtca cagcggcatg cgcacgctgt ttcccatttt tctccacaac ctgcaaccgt
     1741 tctttcttgg aaaggacgca cgtgaactgg accgaatcct cgagagggtc tacatctatg
     1801 gcttcaactt ccgatacaac ggcatcgctc tcggcctgcc cctggctacg atcgagtttg
     1861 caatcctcga tatgctcggc cgcatcgcca agaaacccgt cggtgagctg atcggcgaaa
     1921 tccataatcc cgaagttgcc gtgtacgtag ccaccgaatg gcgcgagaag ccggtagaag
     1981 aatcgatcga actcatcaaa aaggccgtcg ccgagtatcc ggaagcgcgc gcgctcaaga
     2041 ttaaggtggg cgggctcatg ttcatgacca cagacatgga tgccgttggt cctcccggga
     2101 ggaccgagag gatgattccg ctcgtgcgca agacgtttgg ggacgacatg gcgctttatg
     2161 ccgactcgaa cagcttctac tcggtgccgg aagccatccg cgtcggcaaa ttactggaag
     2221 agcataagta cagatatttc gaagagcctg tgatgttcga ccacattgag gagatcaaac
     2281 aggttgccga tgcgctggca ataccggttg ccaacggcga gcaggataac agtttctatg
     2341 ggtttcgctg gcttatcgcc aacgatggca tcgacattgt gcaacccgat aactactact
     2401 tcgggggatt gattcggtcg atgaaagttg cgcgcattgc ggctgcgttt gggaaaagca
     2461 tcaccccgca catgtccggc ggcggactgg gatacgtcta caacattcag tttgtgtccg
     2521 ctgtcccgaa cgccggcgag catcacgaat tcaaagggct gcaaacgaac gtacggttcg
     2581 aatgcaagac ctccccctta aaagtagttg gcgggaagat caaggtccct accggtccgg
     2641 gattcggcgt cgacattgac ccggaatggg tcaagaagca ccagatcgtg aagttgtgac
     2701 ctgtttcttc gtggcgcgcc gcccgggccg gcgcgggagc cccgatgcca cacggacgtg
     2761 atacgttgtg aagccatgat gacacggcgc gatgcgcttg ccttgccggc gacgctcctg
     2821 accgcccacg ctgcgcccgc tctgtcgcgg atgggtatag cggccacctg tttttcggcc
     2881 cagcggccaa aggacacgct cgagttcctg gaattttgcc gctcgctcgg cgcggggggg
     2941 gttcaggcgc cgctgtcatc aacctcgccg gagcacctcg ccgggttgcg gcggagggcc
     3001 gagcagcacg gcatgtacat agaggtcatg gctggcctgc ctcgcggcga ggatgcggag
     3061 actttcgaac gaacgctcat tgcggccggg gaagtgggag cgcgttgcgc ccgtgttgcg
     3121 tgtttgggcg ggcggcgcta cgaaatgttt tccaggctcg acgaatggaa acagtttgcg
     3181 gcgggttcgc gaaaagccat cgcccggacg ttgccgctgg ccgagaagca caggatgccg
     3241 gtggcgatcg aaaaccacaa ggattggact ctcgaggaac tcctcgcttt gctgaaagag
     3301 tactcgagcg aatacatcgg cgtgtgtctg gacacgggaa acaacatcgc cctgctcgac
     3361 gagccgatgg aatttgtgga gcgcctcagc ccgtacgccc tttccacgca cttgaaagac
     3421 atggccgtcg aggagtactc cgaggggttt ctgctcgcgg aggttccatt tggggaaggc
     3481 atgctcgata tgccgcggat cgccgagatc atccggcggg cgcgcccggg gacaaagatg
     3541 acgctcgaga tgatcacccg agatccgctt cgcataccgt gcctgaccga gaagtattgg
     3601 gcaaccttcg cggacacctc cccgcagcgc ctgccggcca tgctgcgaac agtgcgtgcg
     3661 caggcagcca agcggatccc gcgtcctgga acggtcccgc cggagattca ggccaatcgg
     3721 gaagcagaca atgttgccgc ctgcttacgg tatgcgcgcg agcggatggg cctttgaacg
     3781 gcgaagggcg ggcacgagct gtggagtatg atttccaaaa cgggaggggt gaaatgaacc
     3841 gccgccgatg gttatttgcc gccggttccg ctgtcacggc cgggcgggtg cgcgggtcca
     3901 acgaacgggt gaacgtcggg ctgatcggat gcgggggcag aggccgattc gtcgccggcc
     3961 tgatgaagaa aacgccgaag gtggagttta ccgccgtcgc cgacgtctac ctggccaacg
     4021 ccgagcgcgc ccgggagtgg gcaggccccg aagcaaaggc gtaccaggat ttccggcgcc
     4081 tgctggagca caaagaactc gatgcggtgc tcgtcgccac tcccgatcac tggcacgcac
     4141 tggcgacgat cttcgcctgc catgcgggca agcacgtcta cgtggagaaa ccgctggcac
     4201 acaacgttcg cgaggggagg gccatggttg acgcggcaca tcgcgccaac cggctggtgc
     4261 aagcagggac gcagcagcgc tcggcggaac atttccggga ggtttccgaa atcattcgca
     4321 gcggccagct tggggcggtt cacttcgtgc gcatctggaa ctatttcaac ctgtcgcctg
     4381 acggaatcgg ccgtgcggcc gacggcgagc caccggcgga tctcgactgg gacatgtatt
     4441 gcggtgcagc acccaaggtc ccctacaacc cgctacgaca tctcggcacg tatcgctact
     4501 tctgggacta ctccggcgga atgatcaccg actacggaac gcatcgtttt gacacggtgc
     4561 atcagatcat gggggtcgac agcccgcgaa cggtctcagc ctcgggcggc cgttttgcgc
     4621 tgcgggatgc gggcgaggtg cccgatgtgt tgcaggtaac ctacgaatac gcgggcttcg
     4681 tgctgagcta tgagatgtgc atgatcagcg gacacgggct tgggggcaga agcgcaggca
     4741 tgcggtatta caatgcgcat ggcccgctgg accgcccgca tggaattgct ttttatggca
     4801 cgaacggggc actgttctgc gacaggatcg gttatgagat cttcccggac ctcaagaaag
     4861 gctccagtac cgacttccgc atggagcgcc gggcgaagaa tactgtcgac gccactcccc
     4921 ttcacgcggc caatttcatc gacgcgattc gcggcctgaa accgctggct tcaccggtgg
     4981 atatcggcca ttgctcgacc accgttcccc accttgggaa catcgcgttc aagacggggc
     5041 gtaagttggt ctggaatgcg caaaaggagg agatcgagaa cgaccgggag gccagccgac
     5101 tccttagccg gcaggcgcga gcaccgtggc agttgccggt gttgtagtga acagggggtg
     5161 gaatgtgttc gttcgactag taggttgtcc cgtccttccc gccagcaccg ctttctcgcg
     5221 tcacttctcc aaccgcgcat aagcttggcg agcctcctcg gcgaggcgca gcgcttcttc
     5281 cttctcggcg gggttacgaa accgggcgcc ggtgttcagc cagtgaatcg tccctttaac
     5341 gtatttctgt aagtagtgga cagaagaacg ttctcgaacc ttgactccat cgcgcaggaa
     5401 gtagatcgga tttgtatgag cgaacaccgt caaccctcga ggcaggatgc gatttctgct
     5461 gccgggactc tcggctactc gggcggcgag ccaactgctt cgatccaggt cgatcgttcc
     5521 ttccaactcc agggagtaca ccccatccga agtgggacgg tactgattgg gaagggactt
     5581 gacggccgcc gtctctccat tcaccacgat ttcgagactt tgaaaaggca ggatggactt
     5641 cgccgtcgct cgtacccgga ctctgtggct gcccttaaat gaaacagtgt cgccggaact
     5701 gcgaccatcc acctcaaaag ttagcattgg cccgttggtg acgaatccat ttcctgcctt
     5761 cagcccggcc aaccacccct tgtaatccgc ttcgcccacg ggtcgtgcat agagccggtt
     5821 actacccacc ggtatgtagt cgcccatctt atctgtgccg gcgccaatcg gaacttgaaa
     5881 gccggaattc aggtactggt aatagagatc catggaacgc atgacggtaa actccgcctg
     5941 agacatcccg gagttcgtcc aaggccccca atgactggga agctgcgttg ggtcgtcata
     6001 cgtaatcagg tcgatggcat ccaccaaccc atgaacaatg gcgatgggag actcggcccc
     6061 gggcaggttg cagaaatggg cccaggtggt tgtgccccct tgccggcgcg catctcggag
     6121 aatcctggac atcacgaggt taggttcgtt gcggtcctga aacttgcctc catatggcgc
     6181 gaagggctcc acaatccgct tgatgcccag cagaatgacg tgcccgtttt gccagtctcg
     6241 cgattcctga cccacgtata caacgttgcc gggtgtcgac accggatcga ggtcgccggt
     6301 gaatttctct ctgtcgttag tgaaatcact gacgagcagg ttcaggacat tcaggtcttc
     6361 cgcccgcatc tgtgagtgcg cctcagactg tgacaggaaa tgaacatgcg tgtcgccact
     6421 cacatagcct tctttacgca tatcgatcca acgccgcagg cggaaagtca gtggctgact
     6481 tccgtgctga gtcaggtcca tcgcggtttc gatcgggaga gtctcgattc cgcgcctcac
     6541 ctcaaccacc aggcgattgg ggcgggaatt caatacgaac gacccgttca cgtaacatct
     6601 gcgcttgccc aggtactgaa catgggaatg cgggccttca atctcgatcg gctttccttc
     6661 tccatcggaa agcagcagcg ctgctgcaac tggctggccg gtttcgtcat cgaggatggt
     6721 cccttccagc cggtcgacac ccgagcttcg gtaggatacc gacaaaagaa ttgcgaacag
     6781 cggggcgatc accgcccggc gataggaact ctggggcata tctggagcac tcttgcgctc
     6841 tggacgatag tagcaccaac acgtttgccc accgacgtcg aggtggacgt caaccggtgt
     6901 gagttacgac gactcggaaa acccgtgagc gacttcgttg gagttcaagc tcctggcggc
     6961 attctcgcat cgcaaaggcc atctggtcag ccggaaccaa gtggagttgg gtccggcgcg
     7021 tctacaatac ctggtgagcg tgcgaggcgt cgggtgccgc tgcgacggct gaagaggtgt
     7081 tcgttccgcg ccgccgatca ttcggactat gggtggacgt aggaggaaag ctgaagggga
     7141 tccgctgatc cggcacccaa gcgaaggccc gatgggttac acgggtgatg agcccacgca
     7201 gcttctcagt tccgatgccg gcccgtcgcc cgcggagacg ctattgcacg ggatggccaa
     7261 ctgcgtctcg gtgaccagtt cgtatcacgc ggcggcacgc gggcttcccc tcgacgcctt
     7321 caaggtcgac ttcgaaggcg acatagacct acagggcttc gctgatctgg acgacaacgt
     7381 cacaccggca taccagaacg tcaggggcaa gatccacgtg aaggctggag gaagccgcga
     7441 ggcgatcgcc aaattcctga aattcgctac ctcccactcc ccaatgtgcg acagcgtcag
     7501 ccggcctgtc cacacgacct tctcgctggt acataacggc caagcgattt cgcagccggt
     7561 cggcagcgcg gcagcggcgg ggtaggggaa cgggattaag cccgactcac tcctcgcgtt
     7621 cgactcggca ggccaaatca atcgtcagcg gaggcatctc gaaaaagagg tactgttgtt
     7681 gcgtcacggc gctcccgctc cggataaccc ggccgtcttc ggggttcatc ctagtcacgc
     7741 ggaagcggcc tgctccagtg tgaacgtaga tcctctggtc gagcttgtac gggcggctgt
     7801 gatccgcgaa gtgatgaaca tacagaacgg tggtcttttc gttacccaga gcgaaaatgc
     7861 gcaggtcgtt ctgctggctg tacgtgcact ctacggggcg aaggtcgagc ggcagatcgc
     7921 gaataaattc gttcaggacg cggagttgcc gccgggtatc agggccaaga taggcgttcg
     7981 aattcccgcg cggctggccg ggctccgttt ttcttccgga cattccccag tacaggatgt
     8041 tggactcatt catgaacgcg gtccaggccg cgattcgcca cttgaccgga tcaacgttcg
     8101 ataacacgcc cttgttgccg aactcggtgt tcagcactgg cttgccgtac gatttcagcc
     8161 ttccgatctt gtccgcgagc cacgcgtcgg tttccgtggc ggagatactc atgtatccgt
     8221 gccaggtgac gatttcgttc cacttctctg aagagcgcgt gtaattggtc gtgatggggt
     8281 gtttgtaggg atcggcttcg cggatcacca ccgccagtcg cgccaggtaa tcgttggggg
     8341 cgtacgagtc ctcgttgaag agctcccaga tatcgacgaa agcaccgaag cgagcgacga
     8401 tgtaacggag gtaggcctct tgcagcggga ggttggctgc tttgagcgac ttgtagttca
     8461 ccacgtcatc gaccgtgcca aaggcggtca cgtcctttcc ccaaaggctc atgtcctgga
     8521 aggggatcat gatgtgcgac attccgtgtg cgcgttggac agccagtagt tcgtccatct
     8581 tgccggcgat ttcggtatcg tagcggtccg gttggccgcc gaccggaatc agcgtgagcg
     8641 cgcagcccgc tttcgtgcct gcgcctagct gccatcggat gagattcgtc gtgccttgga
     8701 actccttcga ccacatttcg gcgggaactg tcttccagga cccgtcggag ttcggaccat
     8761 caaaaccgac ccgaaagtag ccgcaggtct ggacgccgat gggatagaag ggcgtgccgt
     8821 cctcgaactc aaaacgatac ggattgcgtt tggaaatgcg gacgaagcca cggcggtcgg
     8881 acggaacgca ccgaaatacg cccgatcggt tcacagcgcc tcgcttgccg gagagtttcg
     8941 cttgccagga ccactggccc gcttcgtccg gcgcgaagcg gaccatccac gtcgtgccac
     9001 cgaagtagaa gccggggaca ccgaccctgc gtccggaggg tgaccgaaat acagccgtcg
     9061 cactggcttc cgtgaaagga ttcgagaaca tctcgtctgt cttcagcgaa atttcagaaa
     9121 cttcgtactt accgacggac gtgggttgaa gcagagcagc caaaataaaa gccacgtggc
     9181 agagaaacat tggtcactcc ttttccgctg gctaaacttg cggaagctag gctcactggc
     9241 cggttactta ttcgccccac ttcggcaaca tgtgtttctg gatcttaccc agcgcggtcc
     9301 ggggtagttt ctccacgctt atgaacgccc gcggaatctt aaaggacgcc agactctcac
     9361 ggcagcgcgc ttcgaggatg gccgcgtcga atggccgccg cggaacgaca taagccaccg
     9421 ggacttctcc ccgcagtcgg tccggcacac ccaccactgc cgcctcggcc acttcttcct
     9481 gctcgagcag gaactcttcg atctcgcgcg ggtagatatt gaaaccgccc gagatgatga
     9541 ggtcgctctt cctgcccagc agcgtgtagt agccgtcggt cgaacgctcc gccatgtcgc
     9601 cggtcttaaa ccagccggcg tcgaaggctg cccgagtggc ttcctcccgc cgccagtaac
     9661 cggcgaacac attgggcccg cgcaactgga gctcgccttc gacaatgcgt gccgacacgc
     9721 ccggcagcgg cagtcccacc gttcccgcgc gccgctcacc agcgtacggg ttggagatgt
     9781 tcatgagcgt ttcggtcatt ccgtagcgtt caagaatcgt atggccgaac ttctcgcgga
     9841 actcctccaa cacctgcgcc ggcagcggag ccgatccaga gacgaacagc cgcatgaatg
     9901 ctccgatctg ccgggccgtt tcgggcgcca agtcgagcag gcgcacgtac accgtgggca
     9961 ctccgaaaaa cagagtcggg cggaagtcga ggaactccgc ggacgccttt tgatgctcga
    10021 aacgctccag cagacgcatg cggcatcccc cgatcagcca gcaatgcagc ccgttgccca
    10081 gagcgtgcac gtgaaacagc ggcagcgcca aaagaaaccg gtcgcccgca gtgattcccc
    10141 agcaggtgat cagattgagc gcgttcgccg cgaagttgtt gtgcgtcagg atagcgccct
    10201 tcgacgcccc cgtcgtccct gaggtataga cgatcgctgc cggcgcgtcg ccgtcaagcg
    10261 ggatctccgg gcgctcggcc cgctcgggtg tctggagacc ggagaccggc caaaacggta
    10321 ccgagccggg catctcgtgg gcagcgatca ccgccttcgg ctcagcgtcc gtgaggatgt
    10381 ggagaatctc tcgctcccgg tacaggatgt tgatggggac gaagatcacg cccgacttga
    10441 cgcagcccag gtagatatcg atcagctcca aacagttcga caggtagacg cacagccggt
    10501 cgccggcctc gaggccgcgc tcgccaaaca aggccgccat gcgattacta cggtcgtcga
    10561 tctctccaaa tgtgtacgta cgtccgccaa actccagcgc taccgcttcc ctgcgcccct
    10621 ggaacgagag gtcaaacagt tgtgtcaggt tcatgggttt tctcgaatcc gaacgtggct
    10681 gcgaccgcca gcaacgaaaa accgcccagc gcgaggaagc tcgagcggaa ctcccccgtc
    10741 cagtctgcaa ggtaacctac cgctggggcg cctacaagaa tcatgaggat gccgatcatg
    10801 ttcaccagcc ccatcgccgc gccggcgcgg ccgggaaaca gcgcagccgc ccggttgaag
    10861 acgcccgcat agggcagtcc acagccggct cccagcgcca gaatcgcaat gaacgtcagc
    10921 gccagggact gcccccaagc cagggcggca caggccaccg cgttcatcag cagggcgccg
    10981 cgcaccagag ccgtcatcct catcctccgg agcagccacc cgcccgcagg ccggctcccg
    11041 atgcccagca gtaggaccag cgaacccatc agccccgccg gcttgatcgg catttgaaaa
    11101 tccgccttca gcagaactgt gatccaggct ccgaccacga tcactaaccc gaacgacgcc
    11161 atctgcacca ggcccagcaa ccacagttcc gggctacgga ccatctcgcc gaacttgcct
    11221 gccggacgca cgatatgctc cggccgtggt gcagcgatta cccaccagac caagactgcc
    11281 accgccacaa gcgtcgagct gagaaacgcc ccgcgccatc cgaaggcgcc cagcaactgc
    11341 ggcaccgcga agatcacgaa tcccgagcca agcaccaccg accccccgta gaaaccctgg
    11401 gccacgtgcg ccgctttgcc ggagaacacc gacacgatgt agcgcgcacc
//
LOCUS       DATIFQ010000041         2491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1353677, whole genome shotgun sequence.
ACCESSION   DATIFQ010000041 DATIFQ010000000
VERSION     DATIFQ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353677"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(631..2091)
                     /locus_tag="VLE22_01805"
     CDS             complement(631..2091)
                     /locus_tag="VLE22_01805"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HSB13161.1"
                     /translation="MRKSKASIYERLTNGKLNRQQRKELKRRLYSEDPGLEVVHRNVA
                     GIDVGNGSHYVAVAPGRDPQPVREFGSWTAALHAMADWLKSLGIEHVVMQSTGVYWIG
                     VYEVLEARGFRVCLTNARETKNLPGRKSDVQESQWLMKLHTYGLLRNSFRPAEQIRRL
                     RTIWRLRDRHVHEASREIQHMQKALTTMNVQLANTISDLSGLTGQAIVRAILGGERDP
                     YRLAELRDPHIQASREEIARSLEGRWQEEVLFELQQAVDRYDFCYRQIRDCDARLQQY
                     LAEIPSHERSTQAAEASASTTASDANGKCKKKKAGRKRRQNEPDFDLETELTRICGVR
                     LTAIEGVDVTTVQTWVAELGVDVSAWPSEDHLVSWLNLSPKRQISGGKLIKHEHSHAK
                     NRVANALRMAATALFRSDSYLGARFRSLRGRLGPGKAVKAMAAHLARLMYRMLKHGQA
                     WVDRGAAEHEQRRQQREQFALERKASALGFRLVPAA"
BASE COUNT          425 a          867 c          677 g          522 t
ORIGIN      
        1 aacgcacccc gttgaggtgg tgaatggcta catcgagatc ccggagcgtc cgggcctggg
       61 cacggacctc aatatagagg aaatcctgaa gcacccctat cagcaggaga attatctgcc
      121 cctgttcaaa ccgggctggg agcggcggga acggcaggcc gccgggtaaa aagcgcattt
      181 gtgatgtaat ttcgaaaggg tgaagaatgc aacaattaca ggctacgttc ctctaaacac
      241 agccacttac gccgaatcaa cagtttagac tttggcacgg ctgttgcaac agagaaatgc
      301 agttgaagtg gcgcgaaccg gcggttccgg gaactggaca accagatccc agggccgccg
      361 gttttagttt ttagggggaa gaggcggcct ctctccagaa actcctccgg atgttgttga
      421 ttctgttttg tgggtttacc ttgccgcgcg ctttttgcgc ggtaatcaag tgcgacgcgc
      481 gccgaagcga agcgaggtcg cggcgcttct gcgccccctc gagggggcaa cgggggtgtc
      541 caatggttct gcgcgcgcac gaatcctcgg ccaccacggc gcgcccgctg cgcgcagcac
      601 cttctcttcc acaatttgtc cggctctgga ctatgccgca gggacgaggc gaaagccgag
      661 tgctgacgcc ttccgttcca aggcgaattg ctcgcgctgt tgtcgccgct gttcgtgctc
      721 cgccgctccc cggtccaccc acgcctgccc atgcttcagc atgcgataca tgagccgcgc
      781 caggtgcgcc gccatcgcct tcaccgcctt gcccgggccc agacgccccc gcaacgagcg
      841 gaaccgcgct cccaggtaac tgtcgcttcg gaacagcgcc gtggccgcca tgcggagcgc
      901 gttcgccact cgattcttcg cgtggctgtg ttcgtgtttg atgagcttgc caccgctaat
      961 ctgccgtttc ggtgacaggt tcagccaact caccaggtgg tcttcgctgg gccaagccga
     1021 aacatccacg cccagttccg ccacccaggt ttgaaccgtg gtcacatcca cgccttcaat
     1081 ggccgtcagc cgcaccccgc agatgcgggt cagctctgtt tccaggtcga agtcaggctc
     1141 gttctgccgc ctcttgcgtc ccgctttctt cttcttgcac ttaccgttcg catcgctggc
     1201 cgttgtgctc gcgcttgctt ccgccgcttg ggtgcttcgc tcgtggctcg gtatttccgc
     1261 caggtactgc tgcaagcgcg cgtcgcagtc ccggatctgc cggtagcaga agtcataccg
     1321 gtccacggcc tgctgcagct caaacaggac ctcctcctgc cacctccctt ccaaactgcg
     1381 cgcgatttct tcccggctcg cttggatgtg tggatctcgc agctccgcca gccggtaggg
     1441 gtcccgctct ccgcccagga tcgcgcgcac aatcgcctgc cccgtcagcc cactcaggtc
     1501 gctgatcgtg ttggccaact gcacgttcat cgtagtcagg gctttctgca tatgctggat
     1561 ctcccgactt gcctcgtgca cgtgccgatc ccgcaaccgc cagatcgtcc gcaaccgtcg
     1621 gatctgctcc gcgggccgga acgaattgcg caacagcccg taggtatgca gcttcatcag
     1681 ccactggctc tcctgcacgt cgctcttccg ccccggcagg ttcttggtct cccgcgcatt
     1741 cgtcagacac acccggaatc cccgcgcctc caatacctca tacaccccga tccaatacac
     1801 ccccgtcgac tgcatcacca cgtgctcgat ccccagcgat ttcagccaat ccgccatcgc
     1861 atgcagcgcc gctgtccaac tgccgaactc ccgcacgggt tgcgggtcac ggcccggcgc
     1921 cacggccaca tagtgactgc cattgcccac atcgattccg gccacgttgc gatgcaccac
     1981 ctccaagccc gggtcctccg agtacagccg tcgcttcagt tccttgcgtt gttgccggtt
     2041 cagcttccca ttcgtcaatc gctcgtagat cgatgccttg ctcttgcgca tgcggtggtc
     2101 tcttcctttt ctggattcgg accagacgct cggctcgatc cgcgatcata ctcaaactct
     2161 ccaccggggt cgggccaggc gccgctctac cggcgactgc ccgccaccaa accatggatc
     2221 gccgcaatcg aagtcaggct gagagacggg cactatggct ccaaatccta tgcgactata
     2281 gcttgccgcg cccggtcctc tcgtactttc gcctatttcg atccctcagc gccagtttca
     2341 gaccccttgg agcccgcgac cgcgcgcccc cttcgctgag aggccgccgc agggcacggc
     2401 cctgccccac tttcagggaa gcggaccgag cgacattgaa ataggaggta tatgacccgt
     2461 atcgccatta tcgcgacgct tgcggcctgc t
//
LOCUS       DATIFQ010000042        12365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2311726, whole genome shotgun sequence.
ACCESSION   DATIFQ010000042 DATIFQ010000000
VERSION     DATIFQ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12365
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311726"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /locus_tag="VLE22_01810"
     CDS             complement(<1..1949)
                     /locus_tag="VLE22_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB13162.1"
                     /translation="MRRLRRLAWTAGISLALAVACVWIGFALYQTSLPPSYEAGEDNP
                     DVTRKYAQPYENAAKQATRQAPSPVAKQDDGLIKVDRPLPSGAPAPRFADVTAEAGLA
                     SFVAFSGTRTSQLPEDMGSGLAWGDFDNDGFDDLFLVSAGGALNLPPEAHARSELYRN
                     LGNGTFSRVTDFPDLRILGMGAAWADCNNDGWLDLVVSGYDTLLLFRNEHGRFIRDGR
                     FPNPKGFWTGVSWGDYDRDGWPDLYVCGYVQYKPQPGTGGLTRQFGLEVPYTLNPSSF
                     EPARNLLFHNVHGSFTEVAAKLGVDNPDGRSLSALWQDFDDDGWPDLYVANDISESKL
                     FLNRKGTFVDAGKQAWVSEYRGSMGLAAGDWDGDGDDDLFISHWVAQGYALYNSLLRD
                     QSRAKVPESEQQPQLHFMDVAETYGIGNISLRYIGWGAEFVDFDSDGWLDLAVANGST
                     FETQGQPRKLVPMEAFLFWNNHGKFFFDLAPWNTSLSVAHVSRGLAVSDYDHDGAADI
                     AIMNLDGGVRLLRNRIPQGNWIEFRLHSRTVGGALGRGEGARLIAEAGGRTFRRSVTT
                     ASYLSQSSRTVHLGLGQAPRVDALEVRWPDGSMQHFAGLAPNQIWDLTQGKGEALPWN
                     PGAGGELLSRTQSPQHNVGSKQAEI"
     gene            complement(2024..4135)
                     /locus_tag="VLE22_01815"
     CDS             complement(2024..4135)
                     /locus_tag="VLE22_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HSB13163.1"
                     /translation="MAQSNIQGSTARTGWASRLTRVAMWTLLVETVTGLAIRFAPFHP
                     AVEWSVLVHTALGALTLIPLAWYCARHWSDYRRQAVSDVVFLGYVAVAALLVCALSGA
                     VVTVQALFGTRSSVLWRNIHLISTFAVLAAALPHLAISLYRRWLAASPAASRTLLVYG
                     GGGLAVALAGIVALPFLYSGTRYVNKFPSDYHFLYGKDRPFAPSLARTVTNGPFDARS
                     LAGSESCGTSGCHDEILAEWKPSAHRYAAMDTVFQGIQKVMAQQNGPESTRYCAGCHD
                     PISLFSGSKNIFTEKLTNAQGYHEGVSCLACHAIRKTDIQGNANYVVIQPREYLWQWA
                     TTGPARVARDFLIRSYPEEHNKLSKRLFKAPEYCAACHKQFIDQEVNRVGWVQLQNQY
                     DNWAASHWNRKGDTKKTVECRECHMPLVPSKDPAAGDALDYNRTERDGKHRSHRFLAA
                     NNLMPALLKLEGWQRHTQLTEQWLKGKLPVPEIRDKWAEGAIVTIRLEHPAEVAPGEK
                     IPIRVILTSNKVGHDFPTGPLDMIQSWLEIQVTDDNGGVLFASGRRDQKNFIEPGTFL
                     FKAEPVDQYGNLIDRHNLWEMVGVRYRRSLFPGYSDTVEYVAMCPSGVASGKSASPSD
                     SWRRRQDFLVPEQQRPGSVHIHAVLQYRKIDQFLLNYVLGEHTGITAPAVDLVEARGT
                     VRIGAPPKPHAAQLASNLGGR"
     gene            complement(4354..7149)
                     /locus_tag="VLE22_01820"
     CDS             complement(4354..7149)
                     /locus_tag="VLE22_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13164.1"
                     /translation="MRLLPVLPLLLLVSAVGAERTASLKVRIWWGHGVAQAQHYWVKP
                     EPADDRLKIRNITGVSLEAGEVEKEGAWETSAGGSDIDGVEFELEYDSTLDKPIDNLH
                     IIWSDLIAAADADTARRLSRDPALRVNPQKITVRMNPEGTRGFSVTVEQLLAEGALWI
                     PSHGIYFTAGEQLRSFEEHRKTLQNGKHRRVLDEIRSEPEATYHKYTALWEDMGHPAY
                     TNPQQRGPGHIVGLTWDSAIAKFGIDRGAGVWNDYGNPDKFQFWFDFADLTKGITPYW
                     KGQSLKDGLPVITTVLEREGVRYEIEQFAYPLNGPPAGRRGDIPMVLFQKVRLTDLTG
                     EPREIPVRMAHRRQFPSHVNSAIEMERQGTTALFREAGYRRALFSVEAPEGELYWSGT
                     RDYQREQKRIDATLILMLPAKGSREFVVKLPSPPVEPEDTAKLTGLDFAAARASTLKF
                     WSDYVARGAQFLVPEKVVNDLFRASLWHALRLPRRHGGEGEDVHIDLPYSNFAYSQTG
                     TPWPVNQAVYVDYMLYDLRGYHGISAEELLAQFRNNQERDGHVNGYANWLVYTPSMLY
                     SVAQNYLLSADRGAFDRLLPFSLKALDWCLAHQREAERLPARVRGLAHGPLNDGTGDG
                     FWAFNQAYMYAGLDIFGKALERAGHLRANEARRAASEIRESIDRAFHAASVRSPLVQL
                     RDHTWIPYVPAEAGTYRRLMDVWYPTDIDTGAVHMIRLRAVDRDGDLANWLLNDHEDN
                     LYFRSWGAANEPVYDQQATAHLWRDDPAAVVRAFYSYMASAFSHSALEPVEHRWTHGQ
                     YFGPPSTDGAWFELFRNMLVHERDDGSLALAMAAPRAWFENGKKIELRGAPTWFGPLT
                     MTIECRSASGTISADVETPSRSRPRALLLRLRHPEGKPMLSVTVNGQDWKDFQPEKDL
                     VRITDPSATKYTVIARY"
     gene            complement(7146..9323)
                     /locus_tag="VLE22_01825"
     CDS             complement(7146..9323)
                     /locus_tag="VLE22_01825"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HSB13165.1"
                     /translation="MRYLIALLLVFRALAAAASDPLASQVTIYRDDYGVPHVVGETEE
                     AAFFGYGYAQAQDHLERMMLQYRDAQGRRAEVQGFEALGDGYLHFIPYEYRWDGDYLQ
                     RLLRTKKGVVENRGKIERPVYAILDGFARGVNTYIRENRARIPAWIDEITAEDVEALE
                     RSQYFRFYSIHDALIKLPGATYDLPRLGSNQWAIAPGKSADGRVIHLEHTHMPWANRF
                     QNYEGHLIVRGKLNTAGISWFGSPVFLDGFNDRITWSATYNEPNIADIYEEKLNPDNP
                     LQYLYDGAWREMRVEQESFRVKGANGIETVTLPLYYTHHGPVVKVDRTARRAWSVRLP
                     NFENVTYTAALLGLMKARDLEQFKTVVRRQWMPRWNLLYSDARDIYWVHNGNVAQRPD
                     GYDWRKPVPGWISATEWGPYLPFESYPQILNPASGFLQNCNNPPWVVTRNSGLKPLDP
                     APYYLRSQPSADAGEEALNTRGERIFQVLSQNRKFSVREMIDLGFDTYVLAAEVVVPL
                     LERAYKAAPPSDSRARRAVDLLAAWDRRSGTDSAAYTYMHFWGKAYQDLFTGAKFSRF
                     NSFERNRIDTASAEEQKMARAALEEAISRIEKRYGRSEVPWGEINFVVRGGKFPLGGT
                     EMYGVLHPDEGPEQENGEIHCDDGWGHLMVVVEGQPKEVWTLLPYGQSEDPSSPHYND
                     QAKLHSRRQAKRFRFTASEILANTKSVWGDPERLKRLAAEEGR"
     gene            9381..12134
                     /locus_tag="VLE22_01830"
     CDS             9381..12134
                     /locus_tag="VLE22_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSB13166.1"
                     /translation="MLSNGRPVLMVALTFAAAVFCRAAERPRIDLNGSWQFRLDPQDS
                     GEKQQWYAGGGEFASTTRVPGAWQAQGVGERSGILRNHYAGAAWYRRTVAIPSAWQGK
                     VVRLHLGGALRHTTLFVNGAKAGEHSGFTTPFSFDVTDHVRPGSENTIVMRVANPGPN
                     VSDSPHDQQAAYPIGMLNYIGNWGGIYGAIHLEATEPVWIDEVAIKPDVEKRQATFRV
                     SFGQRGTERRPPVRVEVTSGQYRGVAEATGASAEIVLPMPDARLWSPEDPYLHTASIR
                     LIENNRELDLVQERFGMREIRTRGRILLLNGKPLYLRGYGDDNIEVINGVPPASKAVY
                     LERLRKAKAFGFNAVRFHSMTPAREFFDAADEAGVLVMAELPVAYTMHFLPHKEFLRA
                     ELERTLRVHRNHPSFLSLALGNELNLNWLKSDAERKEFRETVEEFYRLAKSIDPDRLI
                     LSNDGLMLDPTDMASIFTGAAKDVPTVRHEFGKYYCSLPDISLIPKFTGAVVPTWLEE
                     KKRWVERNGLADLYLLYLRNSQRLQQAGRKYQIERVRLDPDVTGYHYWLIVDYPGGTG
                     EGDSWEEGWFDYFWRPKGVEPSEGARLNSAVLVLIGADVHDRTMWSERGKSFDVFVSN
                     YSEKPIQNGTLRWSLTDGARRVGGGDVAGVNVALGEVARVARLSLPPLPASAARKLDL
                     TVELANGSSIFTNRWSFWAFARNSLLSRAPVPVVSTVRWANISRLYPFINESTESISP
                     DSLLITSALTEDAVRFLESGGKVWLMADRTQFDRAGEGTFFPASGGALGTLIREHPAL
                     QGFPHEGFADLQFYNLVEGAWPLALDGWPRDLTPVAGGIRTTSAFLSKTKNLSRFGYI
                     LEATAGKGKLLITTLRLRENLDEAYPEAVSLFDRLLRYATSEEFRPEFAVPREKLESL
                     IIR"
BASE COUNT         2247 a         3831 c         3907 g         2380 t
ORIGIN      
        1 tggatttccg cctgtttcga gcccacgttg tgttgcgggg actgcgtgcg gctcaagagt
       61 tctccaccgg cacccgggtt ccagggcaac gcctccccct tgccctgcgt gaggtcccag
      121 atctggtttg gcgcgaggcc agcgaagtgc tgcatcgagc cgtctggcca ccggacctcc
      181 agggcatcaa cccgaggggc ttggccgagg ccgaggtgaa cggtgcggct tgattgcgac
      241 aggtagctcg ccgtggtcac agagcggcga aacgtccgtc cgcccgcctc ggcgatcagc
      301 ctcgctccct cgcctcgccc gagggcgccg ccaacagttc tgctgtggag gcggaactcg
      361 atccagttgc cctgcggaat gcggttgcgg aggagacgaa cgcctccgtc gagattcatg
      421 atggcgatat ccgcggctcc gtcgtggtcg taatcggaga ccgcaagccc gcgactgacg
      481 tgcgccacgg agagtgatgt gttccatggg gccagatcga aaaagaactt gccatggttg
      541 ttccagaata ggaacgcctc catcggcacg agcttcctgg gctggccctg tgtttcaaag
      601 gtgctcccat tggcgactgc aagatcaagc cagccgtcgc tgtcgaaatc gacgaactca
      661 gcgccccatc cgatgtaccg aagagagatg ttgccgatac cgtacgtctc tgctacgtcc
      721 atgaaatgaa gttgcggctg ctgttccgac tccggaacct ttgcgcggct ctggtcgcgc
      781 agaagcgagt tatagagcgc gtatccctgt gcgacccagt ggctgatgaa gaggtcgtcg
      841 tccccgtcac catcccagtc cccggcggcg agtcccatgg aaccgcggta ttcggagacc
      901 catgcctgtt tgcccgcgtc cacgaaagtg cccttccgat tgaggaagag cttgctctcc
      961 gagatatcgt ttgccacgta caggtccggc catccgtcgt cgtcgaaatc ctgccagagc
     1021 gcgctcaagc tgcgaccatc gggattatca acccccagtt tcgcggcgac ttccgtgaac
     1081 gagccgtgaa cgttatggaa caggaggttt cgggcaggct caaaggagga agggttaagc
     1141 gtatacggca cttcgaggcc gaactgccgc gtcaatcctc cggtgccagg ctgcggcttg
     1201 tattgaacat agccacagac gtagaggtcc ggccagccgt cgcggtcgta gtcgccccag
     1261 gagacgccgg tccagaaacc cttcgggttt ggaaagcggc cgtcgcggat aaatcgtccg
     1321 tgctcattcc ggaagagcag aagagtgtcg tagccgctga cgacgagatc gagccaacca
     1381 tcgttgttgc agtcggccca agccgcaccc atgccgagga ttcggaggtc gggaaagtcg
     1441 gtcacgcggg aaaacgtccc gttgccgaga ttgcgataga gttccgaccg ggcgtgggct
     1501 tccggaggta ggttcagagc accgcccgca ctgaccagga acagatcatc gaagccgtcg
     1561 ttgtcgaagt cgccccaggc gaggcctgag cccatgtcct ccggaagctg agaagtccga
     1621 gtgccagaga acgccacgaa cgaagcgagt cccgcctcgg ctgtcacgtc cgcgaagcgg
     1681 ggcgcaggag cgccgctcgg aagggggcga tcaaccttga tcaggccgtc gtcctgtttt
     1741 gcgaccggtg agggcgcctg ccgggtggcc tgtttcgcag cgttttcgta aggttgcgcg
     1801 tatttccggg tcacgtccgg attgtcctcg ccagcttcgt acgatggcgg aaggctggtt
     1861 tggtacagtg cgaaaccgat ccagacacac gcgaccgcca aagcgagtga gattcccgcg
     1921 gtccaggcca ggcggcggag acgccgcaca gggcgtcgtt tcgctgccgg cgttccgccg
     1981 gccccgggag ccggaggtgg ttgagggttc gggaccacgg cgttcacctc ccgcccaaat
     2041 tactggcgag ctgggccgcg tgtggctttg ggggggcgcc gatccgtacg gtcccgcggg
     2101 cttctaccaa gtccactgcc ggcgcggtga tgcctgtatg ctctccgagc acgtagttga
     2161 gcaggaattg atcgatcttg cgatattgca gaacggcgtg gatgtgcact gagccgggtc
     2221 tctgttgctc cggcacgagg aagtcctgtc gccggcgcca ggaatcggaa ggactggcgg
     2281 acttcccgga ggcgactccg gacgggcaca tggcgacgta ctccaccgtg tcggaataac
     2341 ccgggaacaa ggagcgacgg tagcggacac ccaccatctc ccagaggttg tggcgatcga
     2401 tcaggttgcc gtactgatcg accggctccg ccttgaacag gaacgttccg ggctcgatga
     2461 aattcttctg gtcgcgccgg ccggaggcga acaggacccc accgttatcg tcagtgacct
     2521 gaatctcgag ccagctctgg atcatatcca gcggaccggt cgggaagtcg tggcctactt
     2581 tgttcgacgt gagaatgacg cggatgggta tcttttcgcc cggagcgacc tcagccgggt
     2641 gctcgaggcg aatggtcacg atcgccccct cggcccattt gtcgcgaatc tccggcacgg
     2701 gcagttttcc tttcaaccat tgctcggtga gttgggtgtg ccgctgccag ccttcgagtt
     2761 tcaacagcgc cggcatcagg ttgttggctg ccagaaagcg gtggctgcga tgctttccgt
     2821 cacgctcggt gcggttgtag tccaatgcgt cgccggccgc agggtccttg gaagggacca
     2881 gcggcatgtg gcactcccgg cattcaacgg tcttttttgt gtcgcctttc cggttccagt
     2941 ggctcgccgc ccaattgtcg tactggttct gcagttgcac ccagccgacc cgattcactt
     3001 cctggtcaat gaactgcttg tggcacgcgg cgcaatactc aggggccttg aacagacgct
     3061 tgctgagctt gttgtgttcc tcgggatagc tgcgaatcag aaaatccctg gctacgcgag
     3121 ccggcccggt tgtcgcccac tgccagagat actcccgcgg ttggattacg acatagttcg
     3181 cgttgccctg gatgtcggtc ttccggatgg catggcacgc gaggcacgag acgccttcgt
     3241 gataaccctg cgcgttcgta agtttttcgg taaagatgtt cttcgaaccc gagaacagag
     3301 agatcggatc gtggcatccg gcgcagtacc gtgtggactc cgggccgttc tgctgagcca
     3361 tcaccttctg gatgccctga aacacggtgt ccatggccgc gtagcggtgg gcgctcggtt
     3421 tccattcggc aaggatctcg tcgtgacagc cggacgtgcc acaggattcg gagccggcga
     3481 gcgagcgggc gtcaaacgga ccattggtga cggtacgggc aagactgggc gcgaacggcc
     3541 tgtctttgcc gtacaggaag tgatagtccg aagggaactt gttcacgtac cgggtgccgg
     3601 aatagaggaa cggcaacgcg acgattccag caagcgccac cgctagcccg ccacccccgt
     3661 agacgagcag cgtccgggac gccgccggcg acgcagccag ccaccgccgg tagagggaga
     3721 tggcaagatg cgggagcgcg gctgcaagga ccgcgaacgt cgagatcaag tgaatgttcc
     3781 gccagagcac cgaggaccgc gtaccgaaca acgcctgcac cgtcacgaca gcgcccgaca
     3841 gcgcacacac gagtagcgcc gcgacggcaa cgtagccgag gaagacgacg tcggagacgg
     3901 cctggcggcg gtagtccgac cagtggcggg cgcagtacca ggcgagtggt atgagggtca
     3961 gcgctccgag cgcggtatgt accagaacac tccactcgac ggcgggatgg aaaggtgcga
     4021 aacgaatcgc cagtccggtg acggtctcga cgagcagtgt ccacattgcg acgcgggtca
     4081 ggcgggatgc ccagcccgtt cttgcggtcg aaccctggat gttgctctgc gccatatcgg
     4141 ttcacaactc ctacgccaac aaaagcgagc ttcagaccca gtttcgggag ccagtatagc
     4201 ggcgggcggc atgcggctga tgtgacttga gtcaccggta gcggtagtgc aaaagtatta
     4261 gtgcagaagg agtttactgc cagccgctcg ctcacggtcg cggttctaac gccgctcgct
     4321 gcgggcgcgg cttccatctc ggtttggggt tcgtcagtag cgcgcgatca ctgtgtactt
     4381 tgtcgcggat ggatcggtga tccggacaag gtccttctcc ggctggaagt ccttccagtc
     4441 ctggccgttc accgtgaccg agagcatcgg cttcccttcc ggatgacgca accggagcag
     4501 cagcgcccgc ggccgcgagc gtgacggagt ctcgacgtcg gctgagatgg tccccgacgc
     4561 gctccggcat tcgattgtca tcgtcagcgg gccaaaccag gtcggcgcgc cgcgaagttc
     4621 gatctttttg ccgttctcaa accacgccct aggagcagcc atcgccagcg ccagcgagcc
     4681 gtcgtcccgt tcgtgaacaa gcatgtttcg gaagagctca aaccatgctc cgtcggtgga
     4741 cggcgggccg aagtattgtc cgtgggtcca gcggtgttcc accggttcga gcgccgagtg
     4801 gctgaatgcg cttgccatgt aactgtagaa tgcgcgcacc accgccgccg gatcgtcgcg
     4861 ccagaggtgt gcggtcgctt gctggtcgta caccggttcg ttagccgcgc cccaactcct
     4921 gaagtacagg ttgtcctcgt ggtcgttgag gagccagttt gcgagatcgc cgtcccggtc
     4981 cactgccctc agacggatca tgtggaccgc ccccgtgtcg atgtcggtgg gataccacac
     5041 gtccatcaga cgccggtatg tgcccgcctc agcggggaca tacggaatcc acgtatggtc
     5101 gcgtaactgg acgagtggcg aacgcacgct ggccgcgtga aacgcccggt cgatcgactc
     5161 gcggatctcc gatgcagccc gtctcgcctc gttcgcgcga aggtgtcctg ctctctccag
     5221 agcctttccg aagatgtcca gcccagcgta catataggct tggttgaacg cccagaagcc
     5281 gtcgccggtc ccgtcgttca gtgggccgtg agccagtcct ctcacgcgag ccggcagacg
     5341 ttccgcttct cgctgatgcg ccaggcacca gtccagcgcc ttcaggctga agggcaggag
     5401 ccggtcgaac gcgccgcgat ccgcagagag cagatagttc tgcgccacgg agtagagcat
     5461 cgacggcgtg tagaccagcc agttcgcata accgttgacg tggccgtcgc gctcctggtt
     5521 gttgcggaac tgagccagca gttcctcggc ggagattccg tggtaaccgc ggaggtcgta
     5581 aagcatgtaa tccacgtaga ccgcctgatt taccggccaa ggcgtccccg tctggctgta
     5641 cgcgaagttc gagtacggca ggtcgatgtg aacgtcctcg ccttccccgc cgtggcgccg
     5701 gggcaaccgg agggcatgcc agagactggc gcggaacagg tcgttcacaa ccttctcggg
     5761 cacgaggaat tgcgcgcctc gcgccacgta gtcagaccaa aacttcaagg tgcttgcgcg
     5821 tgcggctgcg aagtccagtc cggtcagttt tgcggtatcc tccggctcga ccggtggcga
     5881 cggcaacttc acgacaaact cccgagagcc tttcgccgga agcatcagga tcagcgtcgc
     5941 gtcgatgcgc ttctgctcgc gttggtaatc gcgcgtccca ctccaataga gttctccctc
     6001 tggcgcctca acggagaaca gcgcacggcg gtagcccgct tcgcggaaca gcgccgtggt
     6061 tccttggcgc tccatctcga tggcggagtt aacgtgggag ggaaactgcc gccggtgggc
     6121 cattctgaca gggatttcgc gtggttcccc ggtgaggtcc gtaagccgca ctttctggaa
     6181 caggaccatc gggatatcgc cccgcctccc ggccggcgga ccgttcaggg gataggcgaa
     6241 ctgctcaatc tcgtacctga cgccctcgcg ctcgaggacg gtggtgatca ccggcagacc
     6301 atctttgagg ctctgtcctt tccaatacgg tgtgatccct ttggtcaggt cagcaaagtc
     6361 gaaccagaac tggaacttgt ccgggttgcc gtagtcgttc cagacgccgg cgccgcgatc
     6421 gatgccaaac tttgcgatcg cgctgtccca cgtcagtccc acaatgtgcc ccggcccccg
     6481 ctgctgcgga ttggtgtaag ctggatggcc catatcctcc cagagcgccg tgtacttgtg
     6541 ataggtcgcc tcgggttcgc tacggatctc atcaagcacc cggcggtgct ttccgttctg
     6601 cagcgtcttc cggtgctctt cgaacgagcg cagctgttct ccggcggtga agtagattcc
     6661 gtgcgacgga atccacagag ctccctctgc cagcagttgc tcaacggtga cgctgaagcc
     6721 gcgtgttccc tccggattca tccgcaccgt gattttctgt ggattcacgc gcagcgccgg
     6781 atcgcgactc agacgccgcg ccgtgtcagc gtctgccgcg gcgatcaggt cggaccagat
     6841 aatgtgcagg ttgtcgatcg gcttgtccag cgtgctgtcg tattccaact cgaactcgac
     6901 gccgtcgatg tcactgccgc ccgcactcgt ttcccacgca ccttcctttt cgacctcccc
     6961 cgcttcaagc gagacgccgg tgatatttcg gatcttcagc ctgtcgtcgg caggctccgg
     7021 cttcacccag tagtgctgag cctgcgctac gccgtgaccc caccagatgc gcaccttcag
     7081 gctggcggtt cgctcggcgc ccacagcgga gaccagcagg agtaacggaa gaacagggag
     7141 aagtctcatc gcccctcctc cgccgcgagc cgcttcagcc gctcgggatc gccccatacg
     7201 gatttcgtgt tggcgagaat ctcggacgcc gtgaatcgga atcgcttcgc ctgccggcgg
     7261 ctgtgcagtt tcgcctgatc gttatagtga ggggaggaag gatcctccga ctgcccatac
     7321 ggcagcagcg tccagacttc tttcggttgg ccttcgacca cgaccatcag atgcccccag
     7381 ccgtcgtcgc aatggatctc gccgttctcc tgctcgggtc cttcgtccgg atgcaggacg
     7441 ccgtacatct ccgtgccgcc gagcgggaac ttcccgccgc gaaccacgaa gttgatttcg
     7501 ccccagggaa cctcgctgcg cccgtaccgc ttttcgatcc gggatatggc ttcttcgagc
     7561 gcagcgcgcg ccatcttctg ttcttccgcg gacgcggtat cgattctatt ccgttcgaac
     7621 gagttgaacc gcgagaactt cgctcccgtg aagagatcct ggtaagcctt gccccagaag
     7681 tgcatgtagg tgtacgcggc ggaatccgtg ccagaacgcc ggtcccacgc ggcgagtaga
     7741 tcgaccgcgc gccgggcgcg actatccgag ggcggcgccg ccttgtacgc ccgctcgagc
     7801 agtggaacga cgacctcggc agccagcaca tacgtgtcga acccgaggtc gatcatctct
     7861 cgcacgctga acttgcggtt ttgcgaaagc acctggaaga tccgctcccc gcgcgtgttc
     7921 aacgcctcct cgcccgcgtc ggccgacggc tggctcctca ggtaatacgg cgcgggatcg
     7981 aggggcttca gtcccgagtt ccgtgtcacc acccacggag gattgttgca attctggagg
     8041 aagcccgacg ccggattcaa aatctgcggg taactctcga acggcaggta ggggccccac
     8101 tcggtggcgc tgatccatcc cggtacaggc ttcctccagt cgtaaccgtc cggacgctgc
     8161 gccacgttgc cgttgtgaac ccagtagatg tcccgggcat cggaatagag caggttccag
     8221 cgcggcatcc actgccgccg cacaaccgtc ttgaactgct cgaggtcgcg ggctttcatt
     8281 agcccgagca gcgcagccgt ataggttacg ttctcaaagt tcggcaggcg cactgaccat
     8341 gcgcgccgtg cggtgcggtc taccttcacg accggaccgt ggtgcgtata gtagagcggc
     8401 agggtgaccg tctcgatgcc gttcgcgccc ttgacgcgga aggactcctg ctccacgcgc
     8461 atctcgcgcc aagcgccgtc gtagaggtac tggagtggat tgtcggggtt cagtttctcc
     8521 tcgtatatgt cggcgatgtt cggctcgttg tacgtggctg accatgtgat ccggtcattg
     8581 aagccgtcga ggaaaacggg gctgccgaac caactgatgc cggcggtgtt gagcttgcct
     8641 cgcacgatca ggtgcccttc gtagttctgg aagcgattgg cccacggcat gtgcgtgtgc
     8701 tccaggtgaa tcacgcgtcc gtcagccgac ttccccggag cgatcgccca ctggttcgat
     8761 ccaaggcgag gcaagtcgta cgtagccccg ggcagtttga tgagcgcgtc gtggatgctg
     8821 tagaagcgga aatattggct ccgctcgagc gcctcgacat cctcggccgt gatctcatcg
     8881 atccatgccg ggatgcgtgc gcggttctcc cggatgtacg tgttgacacc gcgcgcgaat
     8941 ccgtcgagga tcgcgtatac cggacgctcg atcttcccgc ggttttcaac gacgcccttc
     9001 ttggtcctga gcaggcgttg caggtaatcg ccatcccaac ggtattcgta cggaatgaag
     9061 tggaggtagc cgtcgcccaa ggcctcgaag ccctgcactt cggcgcggcg gccttgtgcg
     9121 tcgcgatact ggagcatcat gcgttccagg tggtcctgcg cctgggcgta gccgtacccg
     9181 aagaaagcgg cctcctcggt ttcgccgaca acgtggggaa ccccgtagtc gtcgcgatag
     9241 atggtgacct gcgaggcaag cgggtccgaa gcagccgccg cgagggcgcg gaagactagc
     9301 aacagcgcaa tgaggtatct cataccggca cacgcgatta tacgcaccac agtccgctcg
     9361 cgtggtagag tctcggcgtg atgctctcga atggccgtcc ggtattgatg gtcgccctga
     9421 cgttcgcggc agcggtcttt tgccgtgccg ctgaacggcc ccgtatcgac ctcaatgggt
     9481 cgtggcagtt ccgcctcgac cctcaggatt ccggagaaaa gcagcaatgg tacgccggcg
     9541 ggggagaatt cgcctccacg accagggttc ccggtgcgtg gcaggcgcag ggtgttggtg
     9601 agcgctcggg catcctgcgt aaccactacg ccggcgcggc atggtaccgc cgcaccgtcg
     9661 cgattccctc cgcgtggcag ggaaaggtgg tgcgcctgca ccttggcggg gcgcttcggc
     9721 acacgacgct cttcgtgaac ggagcgaagg cgggagagca cagcggcttc accacgccgt
     9781 tttcgttcga tgttaccgat cacgtccggc ccggttcgga gaacacgatc gtcatgcgtg
     9841 tcgccaatcc cggcccgaac gtgtccgact ctccgcacga ccagcaagcc gcgtacccga
     9901 taggaatgtt gaactacatc ggcaactggg gcggcatcta cggcgccatc catctggaag
     9961 cgactgagcc ggtgtggatc gacgaagtcg ccataaaacc cgacgttgag aagcggcagg
    10021 cgacattccg agtgtccttc gggcagcggg ggacagaaag gcgtcctcct gtgcgagtgg
    10081 aggtgacttc cgggcaatat cgcggcgtcg cggaggccac gggcgcgtcg gcggaaatcg
    10141 tgctgcccat gccggacgcc cgactgtggt ccccggaaga tccttacctc catacagcct
    10201 cgatccggtt gatcgaaaac aaccgcgaac tcgacctcgt tcaagaacgc tttgggatgc
    10261 gggagattcg gacgcgcggc cggatcctcc tgctcaacgg aaagccgctc tatctacgcg
    10321 gctacggtga cgacaatatc gaggtgatta acggcgttcc gccagcctcc aaggcggtgt
    10381 atctcgaacg gctgcgcaag gcgaaggcgt tcgggttcaa tgccgtccga tttcattcga
    10441 tgacgcccgc ccgggaattc ttcgacgccg ccgacgaagc cggcgtgctg gtcatggccg
    10501 agttgccggt ggcctatacg atgcacttcc tcccgcacaa ggagttcctt cgagcggaac
    10561 tggaacgcac tctgcgtgtc caccggaacc acccctcatt tctgtcactg gcgctcggaa
    10621 acgagttgaa cctgaactgg ctcaagagcg acgccgaacg aaaggaattt cgcgaaaccg
    10681 tagaggagtt ttaccggctg gcgaagtcga tcgaccctga ccggttgatc ctgtcgaacg
    10741 acggcctgat gctcgatccg accgacatgg cgagcatttt cacgggcgcc gccaaagatg
    10801 tccctacggt gcggcatgag ttcggaaagt actattgctc actgccggac atctcgctga
    10861 tcccgaagtt caccggggcc gtcgtgccca cgtggctgga agagaagaag cgatgggtgg
    10921 agcggaacgg attggccgac ctctatctgc tctatctccg taactcgcag agactgcagc
    10981 aggccgggag aaagtaccag atcgaacgcg tgcggctcga ccccgatgtc accggctacc
    11041 attactggct gatcgtcgac tacccaggcg ggaccggcga gggcgattcg tgggaggagg
    11101 gatggttcga ttacttctgg cggcccaagg gtgtggaacc gtccgaaggg gcgcggctga
    11161 acagcgcggt gctggtcttg atcggcgcgg atgtccatga ccgcaccatg tggagtgagc
    11221 gcggcaagtc cttcgacgtc ttcgtctcca actacagtga gaaaccgatc cagaacggta
    11281 cgttgcgctg gagcctgacg gacggcgcgc gccgcgtcgg cggcggggac gtagccggag
    11341 tgaacgtggc gctcggcgag gtggctcgcg ttgcgcgcct gtcgctccca cccctccccg
    11401 cgtcagcggc ccggaaactg gatctgacgg tggaacttgc gaacggttcc tcgattttca
    11461 ccaatcgctg gagcttctgg gccttcgctc ggaattcgct gctcagccgc gcgccggtcc
    11521 cggttgtctc caccgtccgc tgggcgaaca tctcgcggct gtatccgttc atcaacgaat
    11581 cgactgaatc gatttctccg gattcgctgc tcatcacttc ggcgctgacg gaagacgcgg
    11641 tccgtttcct cgaatccggc ggcaaagtct ggctgatggc ggaccggacc cagttcgacc
    11701 gtgcgggcga aggtacgttc ttccccgcat ccggcggagc cttgggaacg ctaattcgcg
    11761 agcaccctgc acttcagggc ttcccccacg aaggtttcgc cgacctccag ttctacaacc
    11821 ttgtggaggg agcatggccg cttgccctcg acggctggcc gcgcgacctg acgccagtag
    11881 ccggcgggat tcgcacgacc agcgcgttcc tcagcaagac gaagaaccta tcccggttcg
    11941 gctacattct ggaggcaacg gcgggcaaag gaaagctgct gatcacgacg cttcgattgc
    12001 gagaaaacct cgatgaggcg tatcccgaag ccgtcagcct gttcgatcga ctactacggt
    12061 atgcgacgag cgaagagttc cggcccgaat tcgccgtccc gcgggagaag ctggagtcac
    12121 tgatcattcg gtgacaagcg gtggagtcgc cgttgcgtct cagatgcgct gtgcttttac
    12181 cgtcaccgga gccagtcaca gcggaagtgg agtaatctct tccaattggg aggcgatatg
    12241 agcgacccga cggcaacggg acggcgcgga ttcctcaaga tgacggcgat ggcgggcggc
    12301 ctggcggcaa gccaatccgc accggcgggc cagacggacc agcaggcaca gaccgcgcag
    12361 tcgat
//
LOCUS       DATIFQ010000043        59359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_12818, whole genome shotgun sequence.
ACCESSION   DATIFQ010000043 DATIFQ010000000
VERSION     DATIFQ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 59359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59359
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12818"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VLE22_01835"
     CDS             <1..957
                     /locus_tag="VLE22_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB13167.1"
                     /translation="LRLPELDAFRRRAVYVPTAWPPASRTVCSIPALTIGRPVSKVKV
                     LNAGDLLLEFGGGTPSGRWSREPNVFSDVRKLGMNAAVIGWSLPYCRALCGDLTSCWW
                     WEMSAQHNSMGERLSEILPNQAISLFETTQFAPWGQSRATLKHVQTVREVLSRAVREA
                     VDRRHDLVYLHLPVPHSPHAYDRRTQGFTLHNAPVAGYWDSLELLDKILFDLRRAMER
                     SGVWDSTAILLSSDHWNRNSRRLDGREDHRVPFLLKMPGQNSGVVVPEQIHTVLSRDL
                     VRAILRRDIITPGDAAAWLEERGRRQLKNLRSSTEEVKQEVL"
     gene            957..3992
                     /locus_tag="VLE22_01840"
     CDS             957..3992
                     /locus_tag="VLE22_01840"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HSB13168.1"
                     /translation="MTTTNTRGRKHGFFGSAWLAGLIVVIAIAVPLIFTGNPAVRASG
                     KPQVLGRLPLLFVPNQGQMDQSMRFMTSAAGASVGFAPESVAFDFSGAEVRLSFVGAN
                     ASPSIEGVDHLPGKFNYMRGADPGHWTTDVPTFAGVRYRLLYPGTSVVYRGDGGRLKS
                     EFHVSAGADPRRIVWRYEGVTSIRVDRLGELVISTHFGELREHRPEACQTISGRRVTV
                     RAAYRLLNELTVGFEIGDYDRNLPLVIDPLLAFSTYLDGSGLDSAKAVAVDASGNIYV
                     TGQTDSLNFPAASALQGQSGGGVDVFVAKLNPSGASLAYCTYLGGGWDDRGFAIATDG
                     SGSAYVTGWTNSPNFPTTSGGRQRVLGGGRDAFIAKLNATGNALVFSTYLGGAGHDSA
                     YGIGVDTSGGVYVAGDTYSTNFPAIGGFQTTNAGRQDAFIAKLKSDGSALLWSTYLGG
                     SGDDAAKALAIDSAGAVYVTGGSDSPNFPVANAIQAVSGGGQDAFVAKLSSDGKALAY
                     STYLGGSGGSVGSNELGTGIRVDVGGSAYVAGVTSSTNFPTVSPLQAYFAGGTWDGFV
                     ARLNVAGTALQYSTYLGGVGIDYPTGLAVDSTGAATVTGYTSSTDFPIVGAVQDRPAG
                     GYDAFVAKLSPQGNGLMESTYFGGNDNDAANGVSLDPAGAIYIAGQTLSANFPLKNSF
                     QPTPAGGTSAFVAKIAAAAPTAVYRAPSGATVLNVFGSAARYSAGGVIVGDPAAAQNS
                     YGDTYAAGRNNIDDVWMNIFQAATLTWKGWIKAGGPAAGNPALAVAEGGEAYVVARDA
                     SYNIRLNQYLPSSGFQGWIALGSGFASDPAAAVAADGTVYVVGRSAAGGVRSGRYVPG
                     AGFQGWVSGGGAAAGKPAIVIGSDGAAYVSVKGTDNAIWIARLSGDLWEPWIKGSGTL
                     GSDPDLAAAGGAVYAAITSPTGLVYVQPFREGPSGGWQGWIWTAGTLNRASVAAAGNR
                     FFVAGRNSLNDFWWYQSGAGWSYYGNLGSPVSDLAAAPK"
     gene            4043..5329
                     /locus_tag="VLE22_01845"
     CDS             4043..5329
                     /locus_tag="VLE22_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HSB13169.1"
                     /translation="MKRNVAWALAGNVVYAGCQWAMLVALAKLCSPETVGQFALGVAV
                     TSPVVLLTGLQLRAALATDVTGTFSLEEYLGLRLIATAAGLATIAAVVVAAGYREETQ
                     WAVLAVGLGKAVESVSDLIYGVLQRNERMDLIARSMILRGAGTLLALGVTVQATGSVF
                     WGALALAAVSLVLLVAYDMPAAARVIPASGRRGVPLGWQAAAAALRPTWRIAGLWRLA
                     RETLPLGFVMMLIGLNRNLPRYFVERSMGERELGIFAAIAYIQVAGSTVVDAVCQSAS
                     PRLAGHHAAGRVRQFRGALAKLAGLASLMGAVGVGAALLAGRQVLALLYGAEYAAESP
                     VLIWLMAVAAVGYVGSVLGYGITATRRFALFPVPYLALTAVAAFCSAYAIPRWGLYGA
                     CATAGLVSLGSCVVPFLVLFQVREESKHVASPSFAA"
     gene            5301..6044
                     /locus_tag="VLE22_01850"
     CDS             5301..6044
                     /locus_tag="VLE22_01850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB13170.1"
                     /translation="MSRLHHLLLERYCDHYARLNSTVDPRVLSWSHRRQMDRTFGSYL
                     AGLPAGSRVLDLGCGSGRLLYWFSLRPGLVPVGVDVSPSQLAVARRALPVVETHCEDG
                     LAFLLRHRGEFSAILCTDVLEHVAGEDDCLRWVEAVRDALRPGGIFLCRTPNAANLLG
                     GYSRYLDLTHVRSFTPSSLLQLLGAAGLAECSIVPYRAGHPLGTLRMGLGNLLHRLFF
                     FLCGHRPAAAYTSNVYAIGYAGARGGTDG"
     gene            6041..7411
                     /locus_tag="VLE22_01855"
     CDS             6041..7411
                     /locus_tag="VLE22_01855"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSB13171.1"
                     /translation="MTAAFPMTVPPTSGSAPARFWMCVLGLSLLGYAVLGKGFAYLSV
                     PPLYAAELLMCLGVLCVACEPGALLRAVVFHRSTLPLYLFMAWGAVRTLPYLSEYGID
                     ALRDGVLWGYGLFAILVLAVASRGVQMLRPMVKAFRRFAWVYPYLTVVALLVQIAGIV
                     LPTLPDTNVPVLSNKTGDIAVHLAGASALFLVGLLPARGILWFICVFASVLTCASGNR
                     GAAVSYLAATAFVLLLSPRARSLAVRFLLPVTLLLGLTLFIDPDVRLHQDREISLRQL
                     AINYASVVTSLSDNPGNTEGTKQWRLMWWGRIVDYTIFGEHFWNGKGFGINLTTADGF
                     RSQSGLLRSPHNVHMTVLARGGVIGLALWLLLQGVWLIQVLRRHFRAARARHFHWSAM
                     FVALGGYWVAFIVNASFDVFLEGPMGGVWFWSLFGFGLAAVRVYDSWAIRMMCATEGR
                     WSPVIA"
     gene            7411..8559
                     /locus_tag="VLE22_01860"
     CDS             7411..8559
                     /locus_tag="VLE22_01860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB13172.1"
                     /translation="MTRKLQLLCIGRDLSPAGAERVQAALFRHLDQSRFSVDLHYMSD
                     GDDLLDEMPAGLAPCFGRIGRVPRKLDAARTLHRLVRAARRSDLIFATQEGSPVYLAA
                     IAGAVARKPVVGWIHGPWSRLRKQAGGWHVLASRILYPRASRLVCVSEGVRRDLESES
                     PSLADRLAVLPNPLDIERIRTQSLEPAPPWATNFPDEGAIVACGRLVPDKGFDLLLRA
                     FAKSASCRRGTRLVVLGEGPERARLESLGIELGLRGRVSLPGHLANPYPVFRRATVFV
                     LSSRVEGVGMVLLEAMTLSVPVVAFDSDAGGPREIFGVQDCGQLVEAGDPGRLADAID
                     RMLGDRALAARCRDWGERRARKFDAERAARRFETLFQSVLDAARSRRS"
     gene            8534..9703
                     /locus_tag="VLE22_01865"
     CDS             8534..9703
                     /locus_tag="VLE22_01865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB13173.1"
                     /translation="MLHVLVAHNYYRHPGGEDAVYEATGSLLEARGNRVTRFTAHNDA
                     TASMSRPALAKAALWNRDVRLRLRATMRAGRPNVAHFMNTFPLISPAAYYTARDEGVP
                     VVQTLHNYRLFCVNGLLFHEGRVCEDCAGKSVSWHGAVRGCYHNSRAASAVAAAVSGF
                     HRLIGTWSAEVDIYIALTEFARRKFLDCGLPASKVVVHPNFLHNDPGSGTGAGGYALF
                     AGRLSAEKGLSVLLDAWQKLGGKTTLKIAGEGPLAAQAASQSRKSVGIEYLGPCSSAE
                     ILALMRDAAFLVLPSECYEGLPMAIVEAFASGLPVIAAGHGSMSTLIDHGRTGLHFRP
                     GDSEDLAANLQWAFSHPAVLARMRTEARAEFEALYTAEKHYRGLVEIYERAMSGN"
     gene            9678..10478
                     /locus_tag="VLE22_01870"
     CDS             9678..10478
                     /locus_tag="VLE22_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HSB13174.1"
                     /translation="MSERCPAIEALDRPVPGFRHMLGMRVDATSYGSVVERVIGWARL
                     AQSRYVCVATVNNVMEAYDSPAFRKVMNSADLVTADGMPLVWGLRLLGERDATRVYGP
                     DLMPHVLAAAESARVPVGFYGGSPEVLERLLAKLRQRYPALRIAYAWSPPFRPLTSEE
                     DARIVCEIGDSGARIVFVGISTPKQERWMAQHRGCVRAVMVGVGAAFDFLAGVKPQAP
                     RWMQRAGLEWLFRLASEPRRLWRRYLRHNPRFLAFFTLELSGLRRFPL"
     gene            10529..11521
                     /locus_tag="VLE22_01875"
     CDS             10529..11521
                     /locus_tag="VLE22_01875"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSB13175.1"
                     /translation="MATDVLVTGAGGFIGHHLVKYLVGKGYRVRGVDIKHPEFEPSAA
                     HEFELLDLRRFDNCLLAARGVGEVYQLAADMGGIGYITAFHAGIARNNTMINVHMLEA
                     ARVHGVNRFLFSSSACIYPQYRQRTPDVTPLKEEDAYPADAEEGYGWEKLFTEKLCQY
                     YHHDYGLETRVVRFHNVYGPLGTYEGGKEKAPAAISRKIALAHDGDEIEVWGDGEQTR
                     SFMYVDDCVEGIHRLMRSNHTDPLNLGTDELVTVNGLVELVARIAGKRIRMRHNYAMP
                     QGVPGRNSDNARLREVLGWEPSTPLREGLGPTYRWIESELRASGRILEPAAAAD"
     gene            11543..13003
                     /gene="wbaP"
                     /locus_tag="VLE22_01880"
     CDS             11543..13003
                     /gene="wbaP"
                     /locus_tag="VLE22_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HSB13176.1"
                     /translation="MAALAEIFEERVRVDCAAPIARARPVATASLLAVSDVISLAAGG
                     CLGFISWSFVNPGVTATPYFVLWPALFVFPLVYASMGLYPAAGLGTVEELRRTVLSTT
                     VVYLAASASIFMLKDVGVYSRGVFVFSWLCSTVLVPLSRALVRWRCAARSWWGVPVVI
                     LGAGETGRLLCSRLLEQPSLGLKPVAFLDDHGAQEPHAGVVVSGPLSEAPAISASLRV
                     RHALVAMPGAERQELISVLEEMGAVFPHLIVIPDLFGMASLWVFPRDLGGVLGLEIRQ
                     NLLLPVNRWLKRGLDLTLSLLFGVLSLPVLALAVVWIKRVSPGPAFYAQQRQGEAGCP
                     IRVLKLRTMFPDAEMRLLEYLDADPDARDEWGRYLKLKNDPRILPGIGHFLRRTSLDE
                     LPQFWNVLRGEMSLVGPRPFPEYHLGKFGGKFRELRCRVTPGITGLWQVSARSNGDLK
                     VQEALDSYYIRNWSLWLDLHILARTLRAVFSRQGAY"
     gene            13071..14279
                     /locus_tag="VLE22_01885"
     CDS             13071..14279
                     /locus_tag="VLE22_01885"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSB13177.1"
                     /translation="MEPEGLIRSTAVALVTLLFFAILTIWIRERWALSALQASVFLLA
                     AGWLWRLLLRPFPVRFGFPHAVFVATAFWGLLQLALGTTVYPHDTWEALLYWAAALAL
                     FFLSSQMLAAAETRARFLRALLYFGFGVSILSVVQLYTSAGRVFWLFPSGYTDKVLGP
                     FVYHNNYAAFIELLLPLAFVECLRDRHRTVLFALIAGVMYASVIASTSRSGAILVTLE
                     VVVLGVLASVRRFLPLRRASMALVLLPVFAAVFTTVFGWRVVWERLQQPDPYVHRREI
                     LLSTLDMARERPGLGFGLGTFPTAYPAYAIFDIGLSVNHAHNDWAEWLAEGGVPFLLL
                     MLSVAFWAVRPAFRSLWGVGILIVFAHALVDYPMQRLGLAAWVFVMMGALDAWAREAR
                     SPEGRRHLSL"
     gene            complement(14269..15711)
                     /locus_tag="VLE22_01890"
     CDS             complement(14269..15711)
                     /locus_tag="VLE22_01890"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HSB13178.1"
                     /translation="MSGRIPIEATDAVRHRIPRDERRGMRTDVVVYASKQILDQISKD
                     LSLEQAMNVATLPGIVGPSLAMPDIHQGYGFPIGGVAATDYRTGVVSPGGVGFDINCG
                     VRLVRTSLTEGEVRPKIRELMNQAFRDVPCGTGGAGHIKIGEKQLNDVLAKGTRWMVE
                     NGFGEDSDAEYSEEGGRLEGADPECVSARAKERGLPQLGTLGSGNHFLEVQFVEKVHD
                     EAAARAMGIEQGQVVVLIHCGSRGLGHQVCTDYLAEMGAAMKRYNITLPDRQLACVPI
                     QSPEGQSYLGAMRAAANFAWANRQAILHFLRGSIWQVFGKGTRIGLIYDVCHNIAKRE
                     RHVVDGEKREVLVHRKGATRAFPPGHREIPASYRNIGQPVLIPGSMGTASWVLVGAEG
                     AMAETFGSVCHGAGRLMSRTAARKGRNAKEVLQALEKQGIVARSETRDGIIEEIPEAY
                     KDVDEVIEVVHAAGLARKVARLRPMGVIKG"
     gene            complement(15714..16124)
                     /locus_tag="VLE22_01895"
     CDS             complement(15714..16124)
                     /locus_tag="VLE22_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HSB13179.1"
                     /translation="MFEILEHTADIGFRAWGPNREELFANAALALESVAIEIENVEAC
                     EVYPVAASGEDDESLLVNWLSEVLYYLDGRRVVMRRFEIERLEPFQVRGRAWGESRDP
                     ERHPAKLVIKGVTYHQLRITQDQTGWSAEVYLDI"
     gene            complement(16117..17388)
                     /locus_tag="VLE22_01900"
     CDS             complement(16117..17388)
                     /locus_tag="VLE22_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB13180.1"
                     /translation="MKYRHRVLALLFLLSIITYIDRVCISVAGPRMQQDLDISPERWG
                     WVVGAFTLSYAVFEIPTGAMGDRIGPRAVLTRIVLWWSVFTTLTGTVSNYLLLLLTRF
                     LFGAGEAGAYPNSSASISRWFPTEERARAQGVVWMASRIGGAVSPLLVVPIQRLYGWR
                     VSFWVFGFLGVIWAAVWYFWYRDHPSDKPGLTAAEMKEIGAAPKGAHHGLPWRQVLRR
                     GNLWYIMLMYHTYCWGAYFYLSWLHTYLQKGRGFTEGAMAVWSTLPFIVGACGNLAGG
                     ATSDLLVRRYGLKVGRRTIGSLGLALSALFMLLTAMTGSKTAAAIFLALGYGSMDCML
                     PVSWAVCLDVGKKYAGAVTGAMNMAGQFGSFLSAVVFGYIVKIWGSYDLPLFPMSAML
                     FISALLFLKIDPTRQLVPEAPQVPAPEPGRV"
     gene            17448..18248
                     /gene="tsaA"
                     /locus_tag="VLE22_01905"
     CDS             17448..18248
                     /gene="tsaA"
                     /locus_tag="VLE22_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HSB13181.1"
                     /translation="MNLRPIGVVRSQVRERKAMPPFGAPASVEVFPEFRDGLHRLEKH
                     SHIWVFAWLDQAERELLQVTPRGVSDSGPEGLHGVFAVRSPARPNPIGLTAAEILRVR
                     DGTIDVDLLDFLDGTKVIDLKPYFCSRDIIFSASNAQIGQPSSREAMRDSLLRQALRF
                     HGELCPDLALAVRIIEHFRAEALGLNEPEEWRITAPVRRGCLIDALIGITRATPGRGG
                     LQFADEDAVRIQRQKTVFLYRLDAIRPADYDRVLSAPDADIFQFEIAP"
     gene            18245..20461
                     /locus_tag="VLE22_01910"
     CDS             18245..20461
                     /locus_tag="VLE22_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13182.1"
                     /translation="MNALTRRTFLGSSSAALASASLSQAISPPAGSADVQGQPVPIDF
                     RYSPLSWQTAYCFPDDPFKSLVGERGDLRYGHPGPGKPIDYFPVVVEFSLLGVEPDTA
                     RRQWLDAPDLPVVHTLITRPDGLLELTAFATHLAGEGRVDNIILEVRPRSGEEAYVRP
                     VITVRTREELQVKVEDSISLVFLATQTEKPFLVADCPLDRRDIGTGWILTLPAAVSTP
                     GKPVVHLLRFPQESQPAERLVAGLKDPSRLTMETRAYWTGWSPFGGDVRWKLAGRHHE
                     FLLACARNIQQAREERNGKLTFQVGPTVYRGLWVVDGHFILEAARYLGYDAEAQQGLE
                     ATWARQDAEGGIFAGGGREHWKDTGIAMFTLVRQAELSGNWDYFRAMQPQILRGVRFL
                     AALRDKAKAEGSANGRYGLLARGFPDGGIAGLRSEFTNTLWALAGLKAVVEASERLRM
                     SGFEEAKSLHDGLRSALSAAMREEMRRHARGFDYLPMLMKEDPQWSAENKWQQPRPQS
                     AQWALSQAIYPGILFEKDDPVVRGHIALMQACTQEDVPAETGWIQHGGLWNYGAAFVA
                     HAYLWAGLTDWARLTFTGFLNHATPLYCWREEQPLRGAMAAGYVGDMPHNWASAECVL
                     YLRHMLALEDGPALRLLPGIGDPELAAAAPCTITASPTRFGRVSLSLEPLPRAAGWRL
                     SFQRQDGPTPGAVQLPATLGSRLMSAEISGAASKRDGAAISVEPGARSWTCVWRAG"
     gene            complement(20468..22804)
                     /locus_tag="VLE22_01915"
     CDS             complement(20468..22804)
                     /locus_tag="VLE22_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB13183.1"
                     /translation="MTATLRGRVQDPTGANVGGAAVTIENALTGYAETARTPEDGAFS
                     ISNIPFREYVLTVTKEGFAVKRQTVALRSNLPVELEIRLELADVAERIAVSAFETVQL
                     VDTEATGTRTQLNLAQIERMAIPVGSRALESVLLSFPGFAADANGAIHPRGAHNQMTY
                     VIDGMPISDQLTGAFGNGIDSAIVQTIELHTGNIPAEYGSKVSGVANITTRSGLNSGR
                     RFFGSTEVSAAQFDHAANVTQFGGQTERFGYFASISAQKSNRFLDQVSIDNLHNGGNA
                     ERAFARLDYQHGPADFLRLNAMVARSGFQLANLRSQHVSGQDQRQLLRDASISLGYIH
                     VIGSSATLDTTASYRTSVAQLFPSAGDTPVTAAQARHLSNVTVANRMSLIQGPHELRF
                     GFDYQHFPVSENFSFGITDARFNPPGSDRFIPTLENFDLSRGGSLFQFSDRASGGLYS
                     GFVQDKFRVGPMLLNLGFRYDRYAFLVEGGQFQPRLGAAWNVKRTGTVFRASYNRIYQ
                     TPVNENLLLSNSERSSVLVPPEVRARLGGALIPIPPERQNVYEAGVQQPLFGKVSVNV
                     MYFRKDIRNLHDNDNFFNTGIIFPTALARAKVRGAEGRIAVPEFRRFSGSVSFTHYSV
                     LVTPPFTGGLFLGNTAIDLLRSGPFVIDHDQKLGVQGVVTYRPHKGIWTTASTRYDSG
                     LVTNPSDPKVVAADPDYADLLPYVDLGANPPRVRPRTLVDMAVGYERVRDDRRTWEVV
                     LQVSNLMNRTALYNFQSIFVGTRLVQPRSLGVRLKWYW"
     gene            complement(23618..24754)
                     /locus_tag="VLE22_01920"
     CDS             complement(23618..24754)
                     /locus_tag="VLE22_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HSB13184.1"
                     /translation="MPSERYEIRVPVKRLLIGLLLTIVPICLAGLWTISQSERALVNT
                     IGTHFRTIAEGTATEIAAYIHERVVNVSVMANEPTVVDAVTAANLAYQGMSEAAIAGR
                     IQRIENEWNTPAAEATVRGMLTSRASRTLLHHRELDPRFLRVTITDARGATVAATHKT
                     LDYFQADEDFWQAIYAKGRGTVNLTDILYDEVTKSNYIGIGVPIVEEGTQRFVGAVDA
                     LVDVTSLFPFVNRVQLGPTSRAALVKEDGTIIAAPGVVFSMNLKSDIYATVKDALQTL
                     HGRQTGYMVADVSGGARKLIGFADTGLKADYRNLGWVVLVSQDSLEAFAPVRTVSRFL
                     AFMALVGLAVVVFLGVYFSLHRRPEMTDIEGALKRPQPAERKTA"
     gene            25171..25815
                     /locus_tag="VLE22_01925"
     CDS             25171..25815
                     /locus_tag="VLE22_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HSB13185.1"
                     /translation="MQSGSNGKEEFVDYYELMQISPNAEHETIQRVYRMLAARHHPDN
                     PETGDAERFLRLNEAYRTLSDHERRAAYDLVYHTRQAEPLRVFELKEFAAGIDGEANR
                     RMGILCLLYSRRRTNPEHPGVSILELETMMWFPREHLVFTVWYLKEKNYIRQDDHSNL
                     MITAEGADYVEENLPSNKTLYKLLKASELGATRSERDVNWHADDQPYPTRAENS"
     gene            25888..26433
                     /locus_tag="VLE22_01930"
     CDS             25888..26433
                     /locus_tag="VLE22_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13186.1"
                     /translation="MRSTIVTPLLLFAVLAASATAAAQTVVEHSLTTGAMGTAAAGGA
                     KGASKSIGSVFGNVSKTLDKAAGSGAAATSAATATEPASSTSSTSETIITSKPPSPRD
                     SAPQKPADPFKIRVGMARVTLVETAGKPMMKTSRVEGTSFVETYYYQGLDDVVVVTLR
                     EGKVTDVIPPPPTDAKESARR"
     gene            complement(26444..26941)
                     /locus_tag="VLE22_01935"
     CDS             complement(26444..26941)
                     /locus_tag="VLE22_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13187.1"
                     /translation="MRQFVTAVVLALPFLALAVFARRFRREGVLMSAAILGWGLWAQL
                     PAEAEEAAAQPYLPLSASFLAFVAAGLATVLTGLLPWDRVASKSARRFGRAGVTAASA
                     GLIAAVAFAVSRGMGLYPTTGGALETVFGAMGALHLGILAGLALASLELLVESRREKR
                     SAAGW"
     gene            complement(26961..28283)
                     /locus_tag="VLE22_01940"
     CDS             complement(26961..28283)
                     /locus_tag="VLE22_01940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSB13188.1"
                     /translation="MVNRRTFLAVAAAAGTASSSPKLAVDGGAPVREKPLRAGFYGTQ
                     YYDDKEKQELLEVLETRRPFRWYGPGNEPPKKVMTFEKEFAARMQTKYALAVTSGTAA
                     LQCAVAGMGIGPGDEVIVPAWTWHSCFNAVVLAGALPVIAEIDETLNIDPEDFERKIT
                     PQTKAVMAVHLQGNPCDMDRVMAVARKHRILVLEDCAQSVGASYKGKPLGSLGDISIY
                     SLQLNKTITAGEGGAVVSNNPELFERAARYHDLGGLRNVHEDWLGGKRLDWFIGSGYR
                     VNEFTGGVLLAQVRKLDTIVNAVRGNARRVYDGIRDLPNLRLRKLPDPDGELGSSVFI
                     EFKTKEQRERFLAAMKAENVPASPPGGSVILPIQPHIEKKITVHPNWPSFNTERGRAI
                     QYGAASCPRTIDILNRFGGVTMDPKFTKRDCDDVIAAIQKVYPAVVGA"
     gene            28517..29575
                     /locus_tag="VLE22_01945"
     CDS             28517..29575
                     /locus_tag="VLE22_01945"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB13189.1"
                     /translation="MNRTLPGQIARVCILPALLLVPALLTGQRPYPCVDSPKIFLYPD
                     FAGQVYVVPDNLCFSNGPGIEVQADGVTIDLAGKTLFCSGSPLGCQGNSLAVGIDVGS
                     HKNVTIKDTIGLGRIEGFHTGILVDKGDRVRIESLTITGTHANFDAFRGDATGILVKD
                     VACPGAILIDGNDVSWHRYGIRLLNSSCVKIQRNIVHHNNNNGGEAYGILLSGLSDPP
                     SVMFGSSGNTITNNTLTYNGFHNTYGSSGEIDAGLVLDGRDTTGNRVSQNTVLSNCGD
                     GIMTRQSASGNTVSNNTVHDNPTQSNGPEQSCSWPSPTQLRNYDLASRGAGENRWSKT
                     NDCGVSFGILPGVCIVSK"
     gene            29820..30221
                     /locus_tag="VLE22_01950"
     CDS             29820..30221
                     /locus_tag="VLE22_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13190.1"
                     /translation="MKLILLVLIPLMVFAHSESDPLCGTAIYEDLVLDHDVTCYNSNG
                     LIIGAEGLTIDLNGKKITCNNGESMGCTGRGLTGIDASKVQAVSIKGPGTVLGFHLSM
                     RVAGTPQLSRAVSARRPVSASRRAPLKTRVD"
     gene            30295..30495
                     /locus_tag="VLE22_01955"
     CDS             30295..30495
                     /locus_tag="VLE22_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13191.1"
                     /translation="MDAAAKLQPELDTEELGLLVELLNRELRDLPAEIHHTRTAAFRE
                     QLRRRMAAAERLMARLRPLLRE"
     gene            complement(30565..31803)
                     /gene="icd"
                     /locus_tag="VLE22_01960"
     CDS             complement(30565..31803)
                     /gene="icd"
                     /locus_tag="VLE22_01960"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HSB13192.1"
                     /translation="MDSYNGVPVPAGGAQIEVKDGKFIVPDKPILPFIEGDGTGPDIW
                     RASKTVFDAAVKLAYGGRRRVEWYEIFAGEKAFNRFNSWLPEDTLAAIQRFHIAIKGP
                     LTTPVGGGIRSLNVSLRQLLTLYVCMRPVRYYPGVPSPMKNPEKLNVVIFRENTDDVY
                     AGIEWRQGTPEAKKVIDFLNSEMGTKIRPDSGVGVKPMSVGGSKRLIRRAIQYALRTG
                     KKSVTLVHKGNIMKFTEGAFRDWGYELVKEEFADACITEAEAGSGVPAGKLLVKDRIA
                     DSMFQQVLLRPDEYEVLATPNLNGDYLSDACAAQVGGLGMAPGANIGDEHGVFEATHG
                     TAPKYAGQDVINPSSLILSGSMMFEFMGWQEVSQLIEKGITETIRQKRVTYDLHRLMG
                     GATKLKTSEFATAIVENMAA"
     gene            32035..33348
                     /locus_tag="VLE22_01965"
     CDS             32035..33348
                     /locus_tag="VLE22_01965"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HSB13193.1"
                     /translation="MENETLTIIDNRTQKSYTVPLHRGNVRAIELRQIKKDAEDFGVM
                     SYDPAFTNTASCQSAITFIDGDKGILRYRGYQIQDLAEHCSFLEVAYLILFGELPTES
                     ELKEWTGKITHHTMIHENIKKFMDGFHHDAHPMGMLVSTVAALSTFYPDARSIHDRQS
                     RKLQILRLIGKMPTIAAYAYRHSLGLPYVYPDNSLTYTENFMNMLWKMAELKYMPNPA
                     LSRALDILFILHADHEQNCSANAMRSVGSSQADPFVSTAAAAAALSGPLHGGANEEVL
                     KMLDEIGSVDNVPAYVDRIKAGHGKLMGFGHRIYKNYDPRANIIKKAAEAVFKVTGRN
                     PKLDIAIELEKIAVQDEYFVKRKLYPNVDFYSGIIYQALGFTPDMFTVLFAIPRTVGW
                     LAQWQEMLEDPEQKIARPRQDYTGHMLREFVPAAQRVATTAAVTV"
     gene            complement(33361..34812)
                     /locus_tag="VLE22_01970"
     CDS             complement(33361..34812)
                     /locus_tag="VLE22_01970"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSB13194.1"
                     /translation="MPSINTFDSKRSRAILFQAALVCGFVLGILLLVETILTYRHVTG
                     TLVLDELTDEAGHYASLLENRARDRSIDSFDTLRSVFDELISEHPEQIAWLREYDRHG
                     AVVGESRGADLTTLPIPYLQSVLTGMERNIRQTRDTPKGPVVVVAMPFRFRFPAERPL
                     FRGNQPPRPGEPRFRAIEVALYLEGASSTFLPLRRNLVVGTAAAIALVASMVVMGVRF
                     RGYLRSRDIEQQLSIARQVQQELLPRGCPGCEEIEFAAECQPAWEVGGDFYDVYPDSG
                     GRTVLVLGDVSGKGLPAALLMGLLHGAVRTASAIAATSSLSEMVTHLNGFLYSQTSRE
                     VFASMFVGVFERDSTTLRYVNAGHNPPLLLRRGDNGPVLSDRLETGGPVLGLLPHAPF
                     EEGKLTVQRGDLLVLYSDGVVETANQQDEEFGEERVLTKVSAHFSEPVSEIQAAVLRA
                     VREHSVSAAPQDDLTVVVARFTPTGGGNGQAAA"
     gene            complement(34825..35571)
                     /locus_tag="VLE22_01975"
     CDS             complement(34825..35571)
                     /locus_tag="VLE22_01975"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HSB13195.1"
                     /translation="MDTPPELRVGTSGWNYSDWRGRFYPEEVASSEYLRYYAGGFDTV
                     EVNYSFYHLPRPATYRKWAEQTPDGFVFAVKASRYITHIKRLKGIEKEWSTFLENAAA
                     LGKKLGPILLQLPPSSRADLRALSRFLKMRRPLPEVKLAFEFRHASWFDPEILEALRQ
                     SRAGLVIANSERYPQAPCVPTADFVYLRFHGPGQLFASAYSNADLEPWARRVRDWLAA
                     GRIVYAYFNNDFHAHAIANARTLLRTSVSG"
     gene            complement(35640..37778)
                     /locus_tag="VLE22_01980"
     CDS             complement(35640..37778)
                     /locus_tag="VLE22_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSB13196.1"
                     /translation="MDCADEIAILRREIGPVAGGEDRLSFDLLKGIMIVEDAVTSPDA
                     IVAAVSRTGMRAEPWRSESDVTRRSFWETRGRTALTAASGLAVLAGFAVHAWLAGGIL
                     EILRPEGTAAGGAPSLAARTLYTIAIVAGGWYVFPKAWAALRRRRPDMNLLMTVAVFG
                     AIAIGEWFEAATVSFLFGLSLALESWSVARARRAVEALLDLAPPLARIQIPGGGERQV
                     RAEEVPTGSRFLVNPGDRIPLDGQVVEGTSEVNQASITGESVPVAKEPGAAVFAGTVN
                     GNGALEVRSTKPASDTVLAHIIRLIEQAQHRRAASEQWVNRFAEYYTPGVMALAAGVL
                     VIPPMLLNAPWHEWIYRSLVLLVIACPCALVISTPVSIVAALATAARAGVLIKGGIYV
                     EAPAHLQAVAIDKTGTLTRGRPVVVEVVALDGYSEARLLQIAAGIESRTDHPLAQALV
                     SYAKIREVPPAEATDVQVLPGKGITARIGGRSFWLGSHRHAAERALKTAEIGEHEERL
                     YGLGRTIVFAGDDARVIGLIAFADEIRPGVRDVVQRLYKAGIRHVVMLTGDNERTAAA
                     VATDAGITEFRAELLPEDKVAAIEELVVRYGPTAMVGDGINDAPAMARSSLGIAMGAA
                     GNDAAIEAADVALMSDDLSKVPWLLAHSRRTLTVIRQNVVASLLVKAVFVLLTFAGYA
                     SLWAAITADMGVSLLVIFNALRLLRANVQS"
     gene            37878..38747
                     /locus_tag="VLE22_01985"
     CDS             37878..38747
                     /locus_tag="VLE22_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB13197.1"
                     /translation="MKWGTDFIPLLLRNGGVPPFHPFRCLSALILSALLLSAPACAQT
                     SGDDDHFRRGSDLFVRQKPMEAAVEFEQSLQRDPRQPRAAKLLGLCYHLLKDNDKAEK
                     AFLLAVKLDPGDADAHFFLGRLHYLKNFFEKARKELDVALKLNPRDPRAHSYLALTLE
                     AMGDNDKALAEHQAAVKWNDKLPKPDFRPHESYGAFLLKLNRLPESEQQLRRAKEIDP
                     SIWETLFELGKLYHRKGDLEASRLHLEAALKAGSADPEDTARIRHLLARVYYGLGRRE
                     DAAKLIGVEIPTP"
     gene            38744..40420
                     /locus_tag="VLE22_01990"
     CDS             38744..40420
                     /locus_tag="VLE22_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB13198.1"
                     /translation="MNRRLGLSGIAVAAAFLMLSGAEKTVVPVVFESAGAKSGLDFVL
                     GNHATPDKHQVETMPAGVGIIDFDNDGFEDVFFINGATVPGLTKKDRTCWNRLYRNNG
                     DGTFRDVTEKAGLSGEGYSMAVAIGDYDNDGWSDLFIAGVNRNILYRNNGDGTFTDVT
                     RKAGVAGDNPQKMWSISAGWFDYNNDGLLDLFVVNYCKWDPLTEPYCGLRKPGYRTYC
                     HPKHYEGLPSTLYRNNGDGAFTDVSAESGIAAHIGKGMGVAFADYDDDGRTDVFVCND
                     TVRNFLFHNDGNGKFTEVGVWAGVSLTEDGAPISSMGVDFRDINNDGRPDLFVSALSN
                     ETFPLFLNMGKGAFRDITYASQIGFLSLPSGGFSAGVFDLNNDGWKDIFVAGSHVMDN
                     EELYSSRASRQPNRVFVNLGGDRFADGKDGSPADVWKPRFHRGCAFADFDNDGRIDVV
                     VSALNEPAELLRNASRPEQYWLQLLLRGVRSNRDAIGAKIRIVSESRAEQHNHVTTSV
                     GYASSSTRRVHFGLGNDSLVKRIEIRWPSGKTQALENIKARQLLRLTEPE"
     gene            40465..41412
                     /locus_tag="VLE22_01995"
     CDS             40465..41412
                     /locus_tag="VLE22_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB13199.1"
                     /translation="MTLTRRTLFGALSASAALAQRRQFANWKPKLGILCRYSKSNIEF
                     AKGDGFASLQLNVGGQLPHDVGDNVIAEVKGQIDRSGLYVSSLMMVVNHIDPDPAKSA
                     AGNSDFAKCIELAGKMRIPYVATMSGKNANIGFDKQIDEIVRVYNEKYFPLCQKHNVK
                     IIWEPWPEGPNVATGPEGYEALFTAFKDSPYVGLLYDPSHLVRQFMDPMQCARDFVDK
                     IWDVHLKDVEIMWHVLRKVGIQPFRNVSWWRYRLPGHGQLNWAEFFTILQEAGYKGAM
                     NIEHEDAFYYPAYEGDDFTPQYKEGFRVTHKFLRQFVPA"
     gene            41644..45117
                     /locus_tag="VLE22_02000"
     CDS             41644..45117
                     /locus_tag="VLE22_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB13200.1"
                     /translation="MILVRFILVFAFLAPGFCFAQATYTAQLRGTITDSTAAVIPSAK
                     VTVTDDATGVSETISSDGQGRYLFASLRPASYTVKVELTGFKTVVRPNVVLRVSQQTD
                     LDFTLEVGQTTETVEVTAESPLLNTVTATLGTEVTNRYIMNMPLLDRALTSLTFLAPG
                     VTEVPGAGADDIRGTNFVSNGQRNGTAEVRLDGALATVPESGEGGNTIVNYQPSVEII
                     QEFKVQNNNYSAEYGNNGGTVVNIVTKSGTNQFHGSGWWFFRRPRFDANDFFSNREGQ
                     PKGDYEKDEWGGSIGGPIKKQKAFFFFDFLKTRDNSPFTLTTTVPTALQKAGDFSQTF
                     NDDGSLQTIFNPRDVHEDENGNWIRAPFPENRIPTDWIDPVARKIVQLYPEATGSGDD
                     VTGRNNYTKKLVSSYPGYQLDTKIDFVPTAKTRIGGRYSRGHSSGSTPSADGQLLDEA
                     TSTGNNQNAVLEHNWTPTPTILWTNRVGIDRSFSHQIASERDPASVGFPPELNGYFGL
                     KRFPEIGPEEYGYLGQSCCVDTVKGQTQWMFSSSLSKVIGGHNLKFGGEQRHSFTNFW
                     QPDYPTGQFAFERSNTMQDVFNPDYMQGNGLASLLVGWGNYAHVGTQPPVADKSKDTG
                     FFIQDDWRVTQRLTLNFGLRYEWSSPFTERFNRLVVVDYNGDTGVDVPELGRLKGTSY
                     LADSKKRTANVDRNNFGPRFGFAFRLNENTVVRGGAGLYYGYNVATNFQYVGTPWYKN
                     VQVYFSKDGGITQYATLSNPFPVGFVGPPGPKYGKLAQWGFDNGYNLNDGLRNAEIYQ
                     WNIGIERKLPSDMLIEINYTANRSTHLPFNGYDGTRNRNFINRENRERYGSDGLAELV
                     DNPFYGFFQGPDAIFNEPDSPYNDPQIERIYLLRPYPQFAGSFGGYPQFIANARYNAL
                     QIRFEKRYSHGLAFTGNYTFSRMTDDNSLGFNPWVGNLQTTGELQDLTDLSTEKSRSG
                     VDTPQRLAFAVSYDLPVGRGKLVGGKMGRGLDAVVGGWKTNAFVTFQSGNPVGVHMAE
                     GRLADGNQRPNVSGSVSGAGVQAVVDGAGNFFNVDAFSDPGDQVPGNAPRYFSDLRTH
                     GIKNIDFSLFKEFYFREDVYLQLRAEFFNFLNTPRFGLPDTSYGSSDFGLITYQANRA
                     RNGQIAIRFVW"
     gene            45233..46693
                     /locus_tag="VLE22_02005"
     CDS             45233..46693
                     /locus_tag="VLE22_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB13201.1"
                     /translation="MADARNGDLKSAESELRKALALSPDDAFCLGTLGAILGMQNKLE
                     ESSQFLQKALKVNPEDWTTRRNLASNQFQLGRLAEARENLDIVLKKNPEDKTAVLLLG
                     MVAEEMGDYATAVGRLTSVPELTAWNPKSIAALARSYYRTGAHEQARATLRQMIDSEG
                     VLLGARIAAGADDFQQAESMLASLPPSFQHGNDTRYELALVRYRAGKYAESEKTLRGL
                     LDSGVQNSDVFNLLAWCHYKQDRMKEAVAAMDLAIDRDPAGESNYYDLGLMLLSRDRA
                     PVALEAARKAVEVASGSYRAHLLKGLVEAKLNRLDDAAISYARASELKPDAPEAVVAL
                     GVVLSAARRYPEAEKTFRSGLEKFPRCAALYQEYGKSLLEQSDFADAVGQARAVRLLE
                     TALSLDPALSEPHFQLGKLALDHDRPREAASHLEIAAKLNPASSRIHFVLSRAYTELQ
                     RPDNAAREMELFEQAKAREEGSAPTASRGETPPLPQ"
     gene            46772..48742
                     /locus_tag="VLE22_02010"
     CDS             46772..48742
                     /locus_tag="VLE22_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13202.1"
                     /translation="MKTNTWTLAALILSASWCFGAEQNWTHFVRIGGHGLALGRAGAI
                     VRSAVGTNVYGIEVDNDITGRYESLLDPAAKLRAIRELADAAHAAGQKAFVYIAGTEC
                     ITANADKAPRSMFKDHPDWVQRKITGEPAVFTGGAAFWIRKGDEDVWISPYASEWRKT
                     YMERVRQIAATGIDGVYVDIPYWMTHFDGWEDSWASFDDHTVAAFKAKTGLDAKKDIK
                     VGDFADPGFRKWVDFRITTLTEFMREIDENVKKVNPQCMTIPEIYPGIEAEAVRVGSD
                     VYEMYRVTDAIAHEYEFDGGAAIGASKTPLDWLHYMTGMYTFRAFAEGKASWMLSYSW
                     DGEKSVDPKEAMKNLFAAQLTAGTNSWDAATHVMSGSNDMATRKAIFGWIAAHEKIFY
                     RPRIPLRPIGVYFSPATRNYFAREWMESYLGTMAMLLQSHREFEIVTPRTLPAFRGEV
                     LVLPDARCLSEKEMQRLEKFAGQGKALILTGETGKLDERAEARPGNAVTRLVRSAGNA
                     AAPRVVAMPKCPGRAYWALLGKQYDRAAASGDPLPDTTQATILRDTLQEFKYQPAVEI
                     AASPFVTAQIAKVDGKVHVFLANFKGLKSKENAVQIPEKGVRISFPADAGQRVHFLPY
                     LDEASEIRSERKDGRLSCILPDIHKAAVVWVD"
     gene            49368..50930
                     /locus_tag="VLE22_02015"
     CDS             49368..50930
                     /locus_tag="VLE22_02015"
                     /inference="COORDINATES: protein motif:HMM:NF006560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB13203.1"
                     /translation="MKKEDDRLGRTTQSSPEQPLSATVPHTPANDPFMSRRDVLAASI
                     ALGSPLLLNHCGVGREKVYDTVIRNGRVMDPESGVDAVTGVGISDGKVLAVSKTALEG
                     RTTLDATGLVVAPGFIDVHQHGQDLENYRLKVMDGVTTALELELGTHDVDRWYAEREG
                     KAPIHFGVSAGHPAARMEVLGEPLKFLPAGDGAHRAATDAEIAQIRGRIEHGLRRGAL
                     AVGMGIQYTPAASRWEILEMFRAAARYGALCHVHLRNMGEREPGSSVEGLEEALAAAT
                     ITGAPLHVVHITSSGLRAADKLLAMIGEARKRGMNVTTECYPYSAAMTALESALFDEG
                     WQQRLGIGYGEVEWAATGERLTAATFAAYRKRGGLAIIHMIPENVVALAVASPLTMIA
                     SDGLIQGGKGHPRVSGTFSRVLGRYVRESRSLSLMGALRKMTLDPARSLERRVPSMRD
                     KGRIRVGAGADLTVFDPAQVLDQATYAEPAKPSAGIRHVLVSGQAVVKDGKLVDGMLP
                     GRAVRAPVDQAS"
     gene            50983..51312
                     /locus_tag="VLE22_02020"
     CDS             50983..51312
                     /locus_tag="VLE22_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13204.1"
                     /translation="MTKKFLMFLGTLAGALPLSGGQIFGTLTEGGKAVAKGVEVVVDC
                     AGEQVKGTTDNFGGYRVNVQKTGRCTFTLTYKGQKPAAAIASLREPVSADFELRMQDG
                     KYKLVRR"
     gene            51384..52217
                     /locus_tag="VLE22_02025"
     CDS             51384..52217
                     /locus_tag="VLE22_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13205.1"
                     /translation="MQILVIPVTTIIVGQYASNWQEKESARRTYADMMNRRAEAESAL
                     RKDMFQTTIVPFLERKTDDMEASVLRLELLASNFHDSLDLSPLFRDVYRTLAAERKQG
                     YEELMMRLTTSAQEVIRREVDAIREASTIRTGTVDVESLKQGPLPLFGEEALNLPGTS
                     SPPRTFRVEVLDFDKQRSEFLVRLTVWNAGSPPVMELQTKAFHLGPFDFPLIDNTRLA
                     GGDRVALTLDGAAEDVAQVMLIYFPGSRASMKDKQYYEDMAAELLRIEERNREKSQLS
                     R"
     gene            52397..53368
                     /locus_tag="VLE22_02030"
     CDS             52397..53368
                     /locus_tag="VLE22_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB13206.1"
                     /translation="MTKADVFPAWGRILSGHRPLLALEITRECPLRCPGCYAYELGHL
                     PNQVPLRELSDLRGDDLVNGVLALVRRFRPIHISIVGGEPLVRYRELDVLLPKLAAMR
                     IEVQLVTSAVRPIPAAWAELPNLHLVVSIDGLQPEHDRRRSPATYARILENISSHRII
                     VHCTVTRKLLVRPGYLRDFVGFWSERQEVRKIWFSLFTPQEGDYSEERLTSKDRSDVV
                     AELSRLRAFPKVDMPEVVLDGYLHPPESPRQCAFAQLTTCISADLATRITPCQFGGRP
                     VCSECGCMASAGLAAIAKYKLGGLIPVSSILSLSRRTGEQLSQMGFV"
     gene            complement(53856..54095)
                     /locus_tag="VLE22_02035"
     CDS             complement(53856..54095)
                     /locus_tag="VLE22_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSB13207.1"
                     /translation="MRRNRLREYLEPFVVLPYDRDLCTKWAEVTVAAQASGRRIDCAD
                     AWIAATALITGAPLITHNRNDCLGVPGLTLISHGQ"
     gene            complement(54103..54744)
                     /locus_tag="VLE22_02040"
     CDS             complement(54103..54744)
                     /locus_tag="VLE22_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB13208.1"
                     /translation="MFRKRGVSQVVRPVCLLAGVLLAAQASHAQMEVAVQAGWSTFRG
                     GKLGETTVLAYPQPVLLDVRLSDGFRAGAAITINQWEYLAHEVGYSYQRTHLKYTVSP
                     KNPPLEITNREVAGEGLGVHTLCYNFVAHAARQLSRVRPFATAGLGGATIRHDRKFVA
                     NVGGGLKIRLSDRYGIRLDVRDYVIQKLRDIQITPEPGWQHNLAYSAGFSLFL"
     gene            complement(54953..56197)
                     /locus_tag="VLE22_02045"
     CDS             complement(54953..56197)
                     /locus_tag="VLE22_02045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSB13209.1"
                     /translation="MRLRFLLSLAVAFVCVMPAQDGFSLRPNDTVVFYGDSITDQRLY
                     TTFVETFVVTRYPKLPVRFVHSGWGGDRVSGGGGGPIDKRLERDVVAYKPTALTVMLG
                     MNDGRYRAFDEEIFREYTAGFEHIVKMMKEAAPGIRMTLIQPSPFDDVTRDPTFPGGY
                     NAVLVRYGEFVKELAQRNGMAVADLNGPVVEALRKANATDPKQAQRIIPDRVHPGPGG
                     HLLMAACLLKAWGAGPLVTAVEIDAAAGRVLEAKNTEVTGVATGKTVSWTQKDAALPM
                     PVNMKDAVIALAVRSSDFVETLNQQTLKVSGLKAARYTLKINGATVGSFDAAQLGRGI
                     NLAELPTPMAAQAASVHELTLKRSGVHQTRWRQVEVPLAEDNLPRVGSILENLDALDA
                     DLEARQRATAQPAACYYELVAE"
     gene            56276..57973
                     /locus_tag="VLE22_02050"
     CDS             56276..57973
                     /locus_tag="VLE22_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HSB13210.1"
                     /translation="MQKSKLLTAIPLLVVLVGAGCGGSSSLRTWHVDSLVKVFPDDKP
                     GTNELSDKTWLVARNGHVNVQVALRSEKAIPQLRVELKAPENDGKKLEAEARWVEYVP
                     IGSNPPGTPFDELVRPAPGLFPDPLMETWPFALKAAITQPVWVTVHAPADAQPGEYKG
                     EVTFFAGQERLKSVDFTVRVTAAAVPAEQKLKVTNWFNFSYDPATPDLRLHYKIDPYS
                     DKHWELLGNLGRVMAAHKQNVILTPVMALAKPSVAGGAIRYDFSLFDKWVETFQKAGL
                     VGTIEGGHLLGRSGGFFTPMVVPAYVIEGGRIVQKGLDTDDPRAEQFLGSFLTALYAH
                     LKEKGWEKQYIQHVHDEPHDREAAIYNRYSKIIHKYLPGVPTIDAVGLDQDISFFADV
                     CDIWVPVLSSFDHQLDKMRAHVEKGGQAWFYTCIGPQGRYLNRFTDLPLVKTQLLHWF
                     NFRYDLTGFLHWGGSSWGPKPFQNVQTVINDNRTLLPAGDNAIVYPNAEKLSVLSSIR
                     LEAMREGIEDYELLVALAGKDQAKAKGLAKTAIPNINDYVRDVPAFRKLQRQLLEAF"
     gene            57990..>59359
                     /locus_tag="VLE22_02055"
     CDS             57990..>59359
                     /locus_tag="VLE22_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HSB13211.1"
                     /translation="MKRATFGALLVVFGLILTLFWGCSAGPGLEFWFVDALVKVFPDD
                     PAGTARLDKAVFPAARNSNVSIQLALRAKQPVGDLYVDALALTGPGQPIETARVRWVE
                     YVVVTTNTQNTPEEELLRKAPALFPDALLTEFPITVKKDQTRSVWVTIHVPASQEPGE
                     YKGELRLRQGTEAIATVPYTLVVHKATVATPIPLAVNNYLNLSDYHLEQFHSCTRGSG
                     PWWNLIANYARFWAGYYQTSIGANAVSLAKPELAGGGLRYNFSDFERYVETWEAAGVK
                     GQIDGGNLMERQRRRDATIMTEAWVVEGGKPVLRSIPWNDPRAQQFLNTFLPALYKRL
                     QELGWTKKYVQGIMDEPNQWEIPAFLDAAAKVRKFMPGVRTIEPVGLRQDVGFMKKTV
                     DIWVPLLGTFDEKLDLLQKHISEDNGEIWYYTCLSPRGKYPNRFVDTSLTKVRLLHWI
                     NYKWNFT"
BASE COUNT        11054 a        18790 c        18115 g        11400 t
ORIGIN      
        1 ctgcggcttc ccgaattgga cgctttccga cgacgcgcgg tctatgttcc gacagcctgg
       61 cctccggcct cgcggacggt gtgttccatc cccgcgctta cgattggccg gccggtatcg
      121 aaggtcaagg tcttgaacgc cggagatctt ctcttggaat tcggcggggg gactccttca
      181 ggacggtgga gccgcgagcc caacgtgttc tccgacgttc ggaagttggg aatgaatgcc
      241 gcggtgatcg gctggtcttt gccgtattgt cgcgcgctct gcggcgactt gacctcctgt
      301 tggtggtggg agatgagtgc ccagcacaat tccatgggag agcgcctttc cgagattctg
      361 cccaaccagg caatcagcct gttcgagacc acccagttcg cgccgtgggg gcagtcccgg
      421 gcgacgctca aacacgtgca aacggttcgt gaggtgctat ccagagccgt cagggaagct
      481 gtggatcgcc ggcatgacct cgtctacctt cacctgccgg ttcctcactc tcctcacgcc
      541 tacgaccgga gaacgcaggg attcacgctt cacaacgcgc cggttgccgg ctactgggac
      601 agcctggaat tgctcgacaa gattctgttc gacctgcgcc gcgcgatgga gcgttccggc
      661 gtttgggatt cgactgcgat tctgctcagt tccgaccact ggaaccgcaa ttcccggagg
      721 ttggacggca gggaggacca tcgcgtacct ttcctgctga aaatgcccgg ccagaactca
      781 ggcgtggtcg ttccggagca gatccacact gtcctcagtc gtgatctggt gcgggccatt
      841 ctccggcgcg acatcatcac gcccggggac gcggccgcgt ggcttgagga gcgaggccgc
      901 cggcaactaa agaacctccg gtcttcgacc gaagaagtta agcaggaggt tctgtgatga
      961 ctacgacgaa cacccgaggg aggaaacacg gatttttcgg atccgcctgg cttgcaggcc
     1021 tcattgtcgt gatcgccatc gccgtcccat tgattttcac cggaaatccg gcggttaggg
     1081 ccagtggaaa gccccaggtt ctcggacgcc tgccgctgct cttcgtcccc aaccaagggc
     1141 agatggacca gtcgatgcgt tttatgacca gcgcggccgg agcgtccgtc ggcttcgcgc
     1201 cggagagtgt tgccttcgat ttctccggcg ccgaagttcg tctgtcgttc gtcggagcga
     1261 atgcatcgcc gtcgatcgag ggagtcgatc acctgccagg caagttcaac tacatgcgtg
     1321 gagcggaccc ggggcattgg accacggacg tccctacgtt cgcgggcgtc cgttaccggc
     1381 tgctctaccc cggaaccagc gttgtctatc gtggcgatgg cggccgcttg aagtccgagt
     1441 tccacgtctc ggcgggcgcc gatcctcgcc gcatcgtttg gcgttacgag ggtgtcacca
     1501 gcattcgggt ggacaggctc ggtgaacttg tgatctccac tcatttcggg gagctgcgtg
     1561 aacaccgccc ggaagcgtgc cagacgatca gcggccgccg ggtaaccgtg cgtgcggctt
     1621 acaggctgct gaatgagttg acggtgggat ttgagatcgg cgactacgac cgcaatcttc
     1681 ctctcgtgat cgatcccctt cttgcgttca gcacctatct tgacggcagc ggattggaca
     1741 gcgcgaaagc ggtcgcggtc gacgcctccg gcaatatcta tgtgaccggg cagacggact
     1801 cgctcaattt cccggccgcc agcgcactgc aaggacagag tgggggcggc gtagatgtat
     1861 tcgtcgccaa actgaaccca tccggcgcaa gcttggccta ctgcacttat cttgggggag
     1921 gttgggacga ccgcggtttc gccatcgcaa cggatggaag cggcagcgcg tacgttaccg
     1981 gttggacaaa ttctccgaac ttccccacta cgtccggcgg caggcagcgg gtccttggcg
     2041 gagggcgcga tgcgttcatc gcgaagctga atgcaaccgg aaacgcactc gtcttcagca
     2101 cctatctcgg cggcgctggc cacgacagcg catatggtat cggggtcgat accagcggcg
     2161 gcgtttacgt cgctggcgac acctactcga ctaacttccc ggctatcggc ggtttccaga
     2221 cgacaaacgc agggcgtcag gacgcattca tcgcgaaact caagtccgac ggctcagcgc
     2281 tcctgtggag cacctatctt ggtggctccg gagacgacgc ggccaaggcg ctagccattg
     2341 acagcgcggg tgcggtgtat gtcaccggcg gctccgactc gccaaacttc ccggtagcga
     2401 acgcgatcca ggcggtgagc ggcggcggac aggatgcgtt cgtagcgaaa ctgagctccg
     2461 atggaaaggc gctcgcgtac tccacctatc tcggcggaag tggtggctcc gtgggttcca
     2521 acgaactcgg aacaggcatt cgagtggatg tcggcggcag cgcctacgtt gccggtgtga
     2581 cgagttccac gaactttcca accgtcagcc cccttcaggc gtacttcgcg ggaggaacgt
     2641 gggacggctt cgtcgccagg ttgaacgtgg cagggacggc gcttcagtac agcacgtacc
     2701 tgggcggtgt cggaatcgac tacccgaccg gacttgccgt ggattcgact ggggcggcca
     2761 cggtgaccgg ctacacgtcg tcgacagact ttccgatcgt cggcgcggtc caggatcgcc
     2821 ctgcgggcgg ctacgacgca ttcgtggcca agttgagccc gcagggcaac ggacttatgg
     2881 agtcgacgta tttcgggggg aatgacaacg acgcggccaa cggggtgtcg ctcgacccgg
     2941 ccggcgctat ctatatcgcg ggtcagacgc tatccgcgaa ctttccgttg aagaactcgt
     3001 tccagccaac gcccgccggc gggacaagtg cattcgtggc aaagatcgcc gcggccgcgc
     3061 cgacggcagt ctaccgggcg cccagcggag caaccgtgct caatgtcttc ggttcagcgg
     3121 cgcgctacag cgccggcgga gtgattgtcg gcgacccggc ggctgcccag aatagttacg
     3181 gcgacaccta cgcggccggc cgcaataaca ttgacgacgt ctggatgaac atcttccagg
     3241 cggcgacgct cacatggaaa ggatggatca aggcgggcgg cccggctgct ggcaaccctg
     3301 cgttggcggt tgcggaggga ggcgaggcct atgtggtggc acgcgatgcg agttacaaca
     3361 tccgcctgaa ccagtatctg ccctcgtccg gctttcaggg ttggatcgcg ctgggaagtg
     3421 gtttcgcgtc ggatcccgcc gcggccgtag ccgccgacgg gacggtctat gttgtcgggc
     3481 gctcggccgc aggcggtgtt cgaagcgggc gttatgttcc aggggccggc ttccagggct
     3541 gggtctcggg cggaggcgcg gcggctggga agccggcgat cgtcattggc tccgacggcg
     3601 cggcttacgt ctctgtcaaa ggaactgaca acgcgatctg gatcgcccgt ctttcgggcg
     3661 atctgtggga gccctggatc aaagggagcg gcacgctggg aagcgatccg gatcttgcgg
     3721 cggcgggtgg cgctgtctac gctgccataa ccagtccgac cggcctcgtc tatgtccagc
     3781 cgttccggga aggaccttcc ggaggctggc agggatggat ttggaccgcg gggacgctta
     3841 accgcgcatc cgtcgcggcg gccggaaacc gcttcttcgt cgccggccgc aactctctga
     3901 atgacttctg gtggtatcag tccggggccg ggtggagcta ctacggcaac ctcggctcgc
     3961 cggtgagcga tctggccgca gctcccaaat gagcgtcgag cgcgcggtgt ccgtttttcg
     4021 tagagccgct ggcgagctat agatgaaacg gaacgtcgcc tgggcgttgg ccggcaacgt
     4081 tgtgtacgcc gggtgccaat gggcaatgct cgttgctctg gcgaagctct gttctcccga
     4141 aacggtcggg cagttcgcgc tgggagtggc ggtgacctcg ccagtggttc tgttgaccgg
     4201 tttgcagttg cgggcggctc tggccaccga tgtcaccggg actttctccc tcgaagagta
     4261 tctcggtttg cgcctgattg cgacggcagc cggattggct acgattgccg cggtggtggt
     4321 cgccgcgggc taccgcgagg agacccagtg ggcggtgctg gccgtaggtc tggggaaagc
     4381 ggtcgaatcc gtgagcgacc tcatctatgg agtgctccaa cggaatgaac ggatggacct
     4441 gattgcgcgg tcgatgatcc tccggggcgc cggcacgctt ctcgctttgg gtgtgacggt
     4501 gcaagctacc ggcagcgtgt tctggggagc cctggcgttg gccgcagttt cgctggttct
     4561 gctggtggct tacgacatgc ccgcggcggc tcgcgtgatc cccgcttcag ggaggcgggg
     4621 cgtacccctc ggatggcaag ctgcggcggc ggcgttgcga ccaacctggc ggatcgccgg
     4681 tttgtggagg ctcgcacgcg aaacgctccc gctcggcttt gtgatgatgc tgatcggctt
     4741 gaatcgcaac ttgcccaggt acttcgtcga gcgctcgatg ggcgaacggg agcttggcat
     4801 cttcgccgcg atcgcttaca tccaggttgc cggttccacg gtggtggatg ccgtttgcca
     4861 gtcggcaagc ccacgactgg ccggccatca cgccgccggg cgcgtccggc agttccgcgg
     4921 cgcgctggcc aagttggccg gactggcgag tttgatggga gccgtcggcg ttggcgccgc
     4981 ccttctggcg gggcggcaag tcctggcgct cctctatggg gccgagtacg ccgcggaatc
     5041 gccggtcctc atatggctga tggcggtagc ggcggtcgga tatgtcggca gcgtcctcgg
     5101 ctacggcatc accgccacga ggagattcgc gctatttccc gtaccctatc tggcgttgac
     5161 ggccgtggcc gccttctgtt cggcctacgc cattccgcgt tggggcctgt atggagcctg
     5221 cgccaccgcg ggtctggtca gtttaggaag ctgcgttgtc ccgttcctgg ttcttttcca
     5281 ggtgcgtgaa gagtcaaaac atgtcgcgtc tccatcattt gctgcttgag cggtactgcg
     5341 atcactatgc gcgcctgaac tccaccgtcg atccgcgggt gctgtcgtgg agtcatcggc
     5401 ggcagatgga tcgcacgttt gggtcttacc tggccgggct gccggcgggc agccgcgtgc
     5461 tggatcttgg atgcggcagc ggtcgcctgc tctactggtt ttccctgcga ccgggacttg
     5521 ttccggtggg ggtcgacgta tctccatcgc agttggccgt cgcgcggcgg gcgttgccgg
     5581 tggttgagac gcattgcgag gatggtctgg cgttcctgct ccgccaccgc ggcgagttct
     5641 ccgcaatcct ctgcacggac gttctcgagc acgtggcggg agaggacgac tgtctccgat
     5701 gggtggaggc tgtccgggat gcgcttcgtc caggtggaat cttcctctgc cggacgccga
     5761 acgccgccaa cttgctcggc ggctacagcc gctatctcga tttgacgcac gtgcggagct
     5821 tcactccgag ctctctcctg caactactcg gggcggccgg tctggcggag tgttccatcg
     5881 tcccctaccg ggcgggccat ccccttggga ctcttcgaat gggtctcgga aacctattgc
     5941 accgcctgtt cttcttcctc tgtggccacc ggccggcggc ggcgtacacg agcaacgttt
     6001 acgcgatagg atatgccggg gcgcggggag ggacagacgg atgaccgccg ccttcccgat
     6061 gaccgtgccc cccacatcgg ggagcgcgcc cgcccggttt tggatgtgtg tgctcgggtt
     6121 gtctttgctc ggctatgccg ttctgggcaa gggctttgcg tacctctcgg ttccgcccct
     6181 gtacgcggcc gagcttctca tgtgcttggg cgtcctctgc gtagcctgtg agcccggtgc
     6241 actcctgcgt gcggtcgtgt tccaccggtc aacactgcct ctgtacctgt tcatggcctg
     6301 gggtgcggta aggacgcttc cgtacctgtc tgagtacggg atagacgcac ttcgcgacgg
     6361 cgttctctgg ggttacgggc tgttcgcgat cctggtgctg gccgtcgcct cgcgcggcgt
     6421 ccagatgctg cggccgatgg tgaaggcctt ccgccggttc gcctgggtgt acccctactt
     6481 gaccgtcgtt gcgttgctgg ttcagatcgc cggtattgtt ctaccgacac tcccagatac
     6541 caatgtgccg gtattgtcga acaagactgg cgacatcgca gtccaccttg ccggtgcgag
     6601 cgcgttgttc ctggtggggt tgcttccggc tcggggaatc ctttggttta tctgcgtgtt
     6661 cgcctcggtt ttgacgtgtg ccagcggaaa ccgaggcgct gccgtcagct accttgctgc
     6721 gactgcattc gtgcttcttc tctctccaag ggcccgttcg ctcgccgtcc gcttccttct
     6781 cccggtgacg ctgctgttgg gattgacgct cttcatcgat ccggatgtca ggctgcacca
     6841 ggaccgcgag atctccctac gccaactggc gatcaattac gcgagtgtcg tgacaagcct
     6901 ttcggataac ccgggaaaca cggaaggaac gaaacagtgg cggctgatgt ggtgggggcg
     6961 gatcgtcgac tacacgatct tcggcgaaca cttctggaac gggaaaggtt tcggaatcaa
     7021 cttgacgacc gcagacggtt ttcgcagcca aagcggcctg ctgcgcagcc cgcacaacgt
     7081 ccacatgact gtcctggccc gaggcggcgt tatcgggctt gctctctggc tcctgctcca
     7141 gggcgtttgg ctcatacagg tgttgcgtag gcactttcgt gccgcgcgcg cccggcactt
     7201 tcattggtcg gcgatgttcg tggcccttgg gggctattgg gttgcgttca tcgtcaacgc
     7261 cagcttcgac gtgttccttg aggggccgat ggggggcgtc tggttctggt cactcttcgg
     7321 attcggactc gcggccgtcc gtgtttacga tagctgggcg atccggatga tgtgcgcgac
     7381 tgaaggccgg tggtcgccgg ttattgcttg atgactcgga aactccagct tctgtgcatc
     7441 ggcagagatc tgagtccggc gggcgccgag cgggttcaag cggcgctttt ccggcatctc
     7501 gaccagtccc gcttctcggt tgatttgcac tacatgagcg atggggacga cctgctcgat
     7561 gagatgccgg ccggtctcgc gccatgtttc ggccggattg gccgtgtccc ccgcaagcta
     7621 gatgccgccc gcacgctcca ccgcctggtc cgagcggctc ggcggagcga tctgattttt
     7681 gccacccagg agggaagccc cgtctatctc gccgcgatcg ccggcgcggt cgcgcgcaag
     7741 ccggtggtgg ggtggatcca tggaccctgg agcagattgc ggaaacaggc gggtggatgg
     7801 cacgtcttgg cgtcgcggat tctgtaccca cgggcgtccc gtctggtgtg tgtttccgaa
     7861 ggggtccggc gagaccttga atccgaatct ccgtcgctcg cggaccggct cgccgtactg
     7921 cccaatcccc tcgacatcga gcggatccga acgcagagcc ttgaaccggc gcccccgtgg
     7981 gccacgaact ttcccgatga aggcgctatc gtggcctgtg gccggctggt gccggacaaa
     8041 ggcttcgacc tgctgcttag agccttcgcc aaatccgcga gctgcaggcg cggaacccgt
     8101 ctcgtggtac tcggagaggg accggagcgg gcccggctcg agagccttgg gatcgagctc
     8161 ggcctgcgag ggcgcgtatc actgccgggt cacctggcga atccctatcc ggttttcagg
     8221 cgcgctaccg tgttcgtgct tagctcaaga gtggaagggg tcgggatggt tcttttggag
     8281 gcgatgactc tgagtgtccc agtggtggcg ttcgacagtg acgcaggcgg tccccgggag
     8341 atcttcgggg tgcaggattg cgggcagttg gtcgaagccg gggaccctgg acggctcgcg
     8401 gatgcaatcg accgcatgct cggcgaccgg gcgcttgccg cgcgttgccg tgattggggc
     8461 gagcggaggg cacgcaaatt cgacgccgag cgcgcggctc gacgctttga aacgctgttc
     8521 cagtcggtgt tggatgctgc acgttctcgt cgctcataat tactaccgcc atccaggggg
     8581 cgaggacgca gtttacgagg ctactggctc gctgctggag gcccgaggga accgcgtgac
     8641 gcgcttcacc gcccacaacg acgcgaccgc ttcgatgagc cgcccggcac tggcaaaagc
     8701 ggcgttgtgg aatcgcgatg tccgtctccg cctgcgagcc acgatgcgcg ccggccgccc
     8761 gaatgtggcc cactttatga acaccttccc gctgatctcg ccggcggcct actacaccgc
     8821 ccgagacgag ggtgttccgg tggtccagac gctgcacaac tacaggctgt tttgtgtgaa
     8881 cggactcctt tttcacgagg gccgcgtatg tgaggactgt gccgggaaat ccgtgtcatg
     8941 gcacggcgca gtgagaggct gctaccacaa ctcacgcgcc gccagcgcgg ttgccgctgc
     9001 ggtgtccggg tttcaccgat tgatcggcac gtggagcgcc gaggtggaca tctatatcgc
     9061 cctgactgag ttcgcgcgga ggaagttctt ggattgcggt ttgcccgcct cgaaagtcgt
     9121 ggtgcatccg aatttccttc acaacgaccc gggttccggg accggcgccg gcggctatgc
     9181 cctcttcgca gggcgcctgt cagccgagaa gggcctctcc gtactgctgg acgcgtggca
     9241 gaaactcggc ggcaagacca cgctgaagat tgccggagag ggccctctgg cggcgcaggc
     9301 ggcttcccaa agccgaaaga gcgtgggaat cgaatatctc ggtccctgct cgtccgccga
     9361 gatcctcgct ttgatgcgag acgctgcttt cctggtgttg ccgtcggaat gctacgaagg
     9421 gctcccgatg gcgatcgtcg aggcgtttgc gagcggcctc cccgtgatcg ccgccggcca
     9481 cggaagcatg agcacgctga tcgatcacgg ccgaaccggc ctgcacttcc ggccgggcga
     9541 ctccgaggat cttgcggcga acctgcaatg ggcgttttcg catcctgccg tactggcacg
     9601 tatgcgtacg gaggcgcggg ccgagttcga ggccctttac accgcggaga agcactatcg
     9661 cggtctcgtc gagatctatg agcgagcgat gtccggcaat tgaggcactg gaccggccgg
     9721 tgccgggttt ccgccacatg cttggaatga gagtggatgc caccagctac ggcagtgtgg
     9781 tggagcgggt catcggctgg gcgcgcctgg cacagtcccg ctatgtgtgt gtggcgacgg
     9841 tcaacaacgt gatggaagca tacgactcgc cggccttccg caaggtgatg aattccgccg
     9901 acctcgtaac ggccgacggc atgccgctcg tctggggact gcggcttctg ggcgaacggg
     9961 atgccacgcg cgtttacggt cctgacctca tgccgcacgt cctcgcggcc gccgaaagcg
    10021 cccgcgttcc agtcggcttt tatgggggca gcccggaggt gctcgagcgg ctgctcgcaa
    10081 agttgcggca acgatacccg gcgctacgga tcgcttacgc atggtcgcct cccttccgtc
    10141 ctctcaccag tgaagaagac gcaaggattg tctgcgagat cggcgattcg ggagctcgaa
    10201 tcgtcttcgt cggcatcagc actccgaagc aggagcgatg gatggcacaa caccgcggat
    10261 gtgtgcgggc cgtcatggtc ggcgtcgggg ccgcattcga tttcctcgcc ggagtgaagc
    10321 cgcaggcacc gcgatggatg cagcgggccg gcctcgaatg gctgtttcgc ctggcgagcg
    10381 agccccgtcg tctctggcgg cgctacctgc gccacaatcc ccgctttctc gcctttttca
    10441 ctctcgagtt gtccggtcta cgtcgatttc ctctctaggg ctaaggttca cccgattccc
    10501 ggtcgatgga ttacctcgga gagcaaatat ggcaacggac gtcttggtaa cgggagcggg
    10561 agggttcatc ggccaccact tggtgaagta cctggtcggc aagggctacc gggtccgcgg
    10621 tgtggacatc aaacaccccg agttcgagcc cagcgccgcc cacgaattcg agttgctcga
    10681 cctgcgcagg ttcgacaact gcctgctggc cgcccgcggc gtcggtgaag tctaccagtt
    10741 agctgcggac atgggcggta tcggctacat caccgcgttc cacgccggca tcgcgcgcaa
    10801 taacaccatg atcaacgtac acatgctcga agccgcacgg gtccacggcg tgaaccggtt
    10861 tttgttctca tcgtcggcat gcatctatcc gcaataccgt caacgaacac cggacgtgac
    10921 ccccctcaag gaggaagacg catacccggc ggatgcggag gagggttacg gctgggagaa
    10981 gctctttacc gagaaactgt gccagtatta ccaccacgac tacggcctgg agacccgggt
    11041 ggtgcgcttc cacaacgtct atggtcccct cggtacctac gaaggcggga aggaaaaggc
    11101 gccggccgcc atcagccgca aaatcgcgct ggcccacgac ggtgacgaga tcgaagtctg
    11161 gggtgacggc gagcagacgc gatcgttcat gtatgtcgac gactgcgtgg agggcatcca
    11221 ccgcctgatg cgttcgaatc acaccgatcc gctcaacctt gggacggacg aactggtcac
    11281 ggtcaacgga ctcgtggagc ttgtggcgag gatcgccggc aaacggatcc gcatgcgtca
    11341 caactatgcc atgccgcagg gtgtgcccgg ccgcaacagc gataacgcca ggctgcggga
    11401 agtactcggc tgggagcctt cgacgcctct tcgggaaggt ctcgggccga cttaccggtg
    11461 gatcgaatcg gagctccggg cttctgggcg catccttgaa ccggcggcag ccgccgactg
    11521 agtgagtcag gacatccgaa cgatggcggc tttagccgag atcttcgaag aacgtgtgcg
    11581 ggtcgattgc gcggcgccca ttgcgagagc gcggcctgtc gccacagcaa gtctgctggc
    11641 ggtttccgat gtcatttcgc tggccgccgg agggtgcctc ggcttcattt cctggtcatt
    11701 cgtgaacccc ggagtcaccg ccactccgta cttcgtcctc tggccggcgc tgtttgtctt
    11761 tccccttgtc tacgcctcca tgggactgta tcccgcggca ggcctcggga cggtcgagga
    11821 actccggcgc acggtgcttt caacgacggt ggtctaccta gccgcatcgg cgtcgatttt
    11881 catgctgaag gacgtcggtg tgtactcccg aggggtcttc gtgttttcct ggctctgctc
    11941 caccgtgctg gtacccctca gccgggcgct ggtgcggtgg cgctgtgccg ccaggtcctg
    12001 gtggggtgtt ccggtcgtta tccttggcgc gggtgaaacc gggcgtttgc tttgctcgag
    12061 actgctggaa caaccttcgc tcggtctgaa gccggtcgct ttcctggacg accatggggc
    12121 gcaggaaccg cacgcaggcg tcgtggtttc cgggcccctg tcggaggcgc ccgcgatttc
    12181 ggcctctctt cgcgttcgcc acgccctggt tgccatgccc ggcgccgaac ggcaggaact
    12241 gatctccgtg cttgaagaga tgggcgctgt cttcccgcac ctgatcgtca tccccgacct
    12301 gtttggaatg gccagcctgt gggtgttccc gcgcgacctc ggcggagtgc tgggcctcga
    12361 gatccggcag aacctgctcc tcccggtgaa ccgctggctg aagcggggac tcgatctcac
    12421 gctctcactt ctcttcggcg tcctctccct tccggttctc gctcttgcgg tcgtgtggat
    12481 caagcgagtg agccccggcc ctgcctttta cgcccagcag cgccagggag aagcgggatg
    12541 tccgattcgc gtgctgaaac tccgcaccat gttcccggac gcggaaatgc ggttgctcga
    12601 gtacctggac gcggatcccg acgcgcggga cgagtggggc cgctacttga agctcaagaa
    12661 cgatccgcgc attctgcccg gcatcggaca cttcctgcgc cgcaccagcc tcgatgaact
    12721 gcctcagttc tggaacgtcc tccgcgggga gatgagcctc gtcggcccgc ggccgtttcc
    12781 ggaataccat ctcgggaagt tcggtgggaa gtttcgtgag ttgcgctgcc gcgtcactcc
    12841 aggcattacc ggcctctggc aggtctccgc acggagcaac ggcgacctga aagtccagga
    12901 agctctggac agctactaca tccggaactg gtctctctgg cttgacctgc acattctggc
    12961 aagaacgctg agagcagtct tctcacgcca gggcgcgtat tgaggcgtcg cggcgcctcc
    13021 cacggtggcg cgccggccac ggctcccctc gggtaagatg gcacttgcgc gtggagcctg
    13081 aaggactcat ccgtagtacc gccgttgccc tcgtcaccct cctcttcttc gccattctca
    13141 cgatctggat ccgggaacgg tgggcgctca gtgcccttca agcgagcgtc tttctcctgg
    13201 ctgcgggctg gctctggcgc ctcctgctgc gtcccttccc cgtgcgcttc ggcttcccgc
    13261 acgccgtatt cgtcgccacg gccttctggg ggctcctgca gcttgctctc gggaccacgg
    13321 tgtacccgca cgacacctgg gaggctttgc tttactgggc ggcggcgctt gccctgttct
    13381 tcctctcctc tcagatgctc gccgccgcgg aaacacgagc gcgttttctc cgggcgctgc
    13441 tgtattttgg atttggggtc tcgatcctct cggtcgtcca gctctataca tccgccggcc
    13501 gtgtcttctg gctctttccc agcggttata ccgacaaggt gctgggtccg ttcgtttacc
    13561 acaacaacta cgcagcgttt attgaactcc tgctaccgct cgcgtttgtc gaatgtctcc
    13621 gggaccggca tcgtacggtc cttttcgcct taatcgcggg cgtcatgtac gcctccgtga
    13681 ttgcgtcaac atcccggtcc ggagcgattc tcgtcacgtt ggaagtcgtc gtgctcggcg
    13741 ttctggcgtc ggtccggcga ttcctgccgc tgcggcgcgc ctcgatggcg ctcgtcttgc
    13801 tccctgtgtt cgcggctgtc tttacgaccg tattcggctg gcgtgtcgtg tgggaacggc
    13861 tccagcagcc ggatccgtac gtccaccgcc gggaaattct gctctccacc ctcgacatgg
    13921 cgcgggaacg cccggggctc ggtttcggcc tgggaacgtt tccgacagcc tatcccgcct
    13981 atgccatctt cgatatcggc ctctccgtca atcacgccca taacgactgg gcggagtggc
    14041 tggccgaagg tggcgttccg ttcctgctgc tgatgctgtc ggtcgcattc tgggccgttc
    14101 ggccggcctt ccgttctctc tggggagtcg ggatcctgat cgtgtttgca cacgcgctcg
    14161 tggactaccc catgcagcgc ctcgggctcg ctgcgtgggt cttcgtgatg atgggcgcac
    14221 tggatgcgtg ggcgcgcgaa gcgcgatcac ccgaagggcg caggcacctc agcctttgat
    14281 tactcccatc ggccgcagcc gcgccacttt gcgcgcaagc ccggccgcgt ggaccacctc
    14341 gatcacctcg tccacgtcct tgtaggcttc cggaatctcc tcgatgatgc cgtcgcgcgt
    14401 ctcgctgcgc gccacaatgc cctgtttctc gagcgcttgc aggacctctt tggcgtttct
    14461 gccttttcgg gcggcggtgc ggctcatcag ccttcccgca ccgtgacaga cgctgccgaa
    14521 cgtctccgcc atcgcgccct cggcgcccac cagaacccag gaggccgtcc ccatgctccc
    14581 aggaatcaac accggctgtc cgatgttccg gtaggaagcc gggatctccc ggtggcccgg
    14641 cggaaaggcc cgggtcgcgc ccttccgatg cacgagcacc tcccgtttct ccccgtcgac
    14701 cacgtggcgc tcccgcttcg cgatgttgtg gcaaacgtca tagatcaggc cgattcgggt
    14761 cccctttcca aatacttgcc aaatcgatcc tcgcaggaag tgcaggatcg cctgccggtt
    14821 cgcccaagcg aaattcgcgg ccgcccgcat tgctcccagg tatgactgcc cctccggaga
    14881 ctggatcggc acgcaggcca gttgccgatc gggaagtgtg atgttgtagc gcttcatcgc
    14941 ggcgcccatc tccgccaggt agtctgtgca cacctgatgt ccgagcccgc gggagccgca
    15001 gtggatcaga acgacgacct gcccttgttc gatccccatc gccctggccg ctgcctcgtc
    15061 gtggaccttc tcgacgaact ggacctccag aaaatggttt ccgctgccga gcgttccgag
    15121 ttgcggcagc ccgcgttcct tggcgcgggc ggaaacgcac tcgggatccg cgccttcgag
    15181 ccggccgcct tcttccgaat attcggcatc gctgtcctcg ccgaagccgt tctccaccat
    15241 ccagcgcgtg cccttcgcga gcacgtcgtt cagttgcttc tcgccgatct tgatgtgacc
    15301 ggcgccgccg gtgccgcagg ggacgtcccg gaacgcctgg ttcatcagct cccggatttt
    15361 cggccgcacc tcaccctccg tcagtgacgt ccgcacgagc cgcacaccgc agttgatgtc
    15421 gaaaccgaca ccgcccggcg acacgacgcc cgtccggtaa tcggtcgctg ccaccccgcc
    15481 gatcgggaat ccgtaacctt ggtgaatgtc cggcatcgcc aggctcgggc ccacgatgcc
    15541 cggcagtgtg gcgacattca tcgcctgttc gagcgagaga tccttgctga tctgatcgag
    15601 gatctgcttg ctcgcgtaca ccacgacgtc cgttcgcatg ccccggcgct cgtcgcgtgg
    15661 aatgcggtga cgcaccgcgt ctgtggcctc aatcggaatc ctgccgctca tgatcagatg
    15721 tccaggtaga cttcggcgct ccagcccgtc tggtcctgtg tgattctcag ttggtggtag
    15781 gtgacgccct tgatgaccag tttcgcggga tgacgctcgg gatcgcgaga ctctccccac
    15841 gcccggcccc ggacctggaa cggttccagt cgctcgatct cgaaccggcg catgactacg
    15901 cgcctcccgt ccaggtaata caaaacctcg ctcaaccagt ttaccagcaa cgattcgtcg
    15961 tcttcgcccg aggccgcgac cggatacact tcgcacgcct cgacattctc gatctcgatg
    16021 gccacggact cgagagcgag cgcagcgttg gcaaacaact cctccctgtt cgggccccag
    16081 gcgcggaagc cgatatccgc cgtgtgttcc agaatctcaa acacgtccag gttccggcgc
    16141 cggaacctgc ggcgcctcag gcactaactg gcgcgtcgga tcgatcttca gaaacagcag
    16201 cgcgctgatg aatagcattg ccgacatcgg gaacagcggg agatcgtagc ttccccatat
    16261 cttgacgata tagccgaaca cgaccgccga caggaatgag ccgaattgtc ccgccatgtt
    16321 catcgctccg gtcaccgctc ccgcatattt ctttccgacg tccaggcaga ccgcccacga
    16381 caccggcaac atgcagtcca tgctgccgta cccgagcgcg aggaagatcg ccgccgccgt
    16441 cttgctccca gtcatcgccg tcagcagcat gaacagcgcc gacagcgcca aaccgagtga
    16501 accgatcgtt cgccggccca ccttcagccc atagcgccgg actagtaagt cgctggtcgc
    16561 gcctcccgcg aggtttccgc aagccccgac gataaatgga agcgtcgacc agacggccat
    16621 cgccccctcg gtgaatccgc gtcccttctg caagtaggta tgaagccagg aaagatagaa
    16681 ataggcgccc cagcagtagg tatggtacat cagcatgatg taccagaggt tgcccctcct
    16741 gagcacttgt cgccatggaa ggccatggtg cgcgcccttc ggcgctgcgc cgatctcctt
    16801 catttccgcc gcggtcaggc ccggcttgtc ggacggatgg tcccgatacc agaagtacca
    16861 gaccgcagcc cagatgacac cgagaaagcc gaaaacccag aacgaaaccc gccaaccgta
    16921 caaccgttga atcggcacga caagcaacgg cgagaccgcc cctccaatgc ggctggccat
    16981 ccatacgacg ccctgcgcgc gcgcccgctc ttcggtcgga aaccatctgg aaatgctggc
    17041 cgaactgttc gggtaggccc ccgcctcgcc cgcgccgaac agaaaacgag tgagcaggag
    17101 caggaggtaa ttggacacgg tgcccgtcag tgtggtgaac acggaccacc acaaaacgat
    17161 ccgcgtcaac acggcgcgcg gtccgatgcg atcgcccatg gccccggtcg gaatctcgaa
    17221 cactgcgtac gaaagggtga aggcgccgac cacccaaccc cagcgctccg gtgagatatc
    17281 caggtcctgc tgcatcctcg gcccggcgac cgaaatgcag acccggtcga tgtaggtaat
    17341 gatggagagc agaaacagga gcgccagtac gcggtggcga tatttcataa cgtcgcaatt
    17401 ataatgcaca gcgcgcgcca gcacggtgcc ggctcccgga ggaatcgatg aacttgcggc
    17461 caatcggagt ggttcgctct caagtccgtg aacgtaaggc gatgccgccg ttcggcgctc
    17521 cggccagcgt cgaggtgttc cccgaattcc gggatgggct gcaccgtctg gagaagcact
    17581 ctcacatctg ggtcttcgcc tggctggatc aggcggagcg cgagctcctc caggtcacgc
    17641 cacggggtgt ctcggactcc ggtccggagg gactccacgg cgtcttcgcc gttcgctctc
    17701 cggcgcggcc aaacccgatt ggcctgaccg cggccgagat tctccgtgtg cgcgatggga
    17761 cgatcgacgt cgatctgctc gacttccttg acggcaccaa agtcatcgac ctcaagccgt
    17821 atttctgcag tcgagacatc atcttctccg ccagcaacgc gcagatcgga cagccatcct
    17881 cccgcgaagc catgcgcgac tccctgttgc gccaggcgct gcgcttccat ggtgagctgt
    17941 gtccggacct cgcgctcgcc gtccggatta tcgagcactt ccgggccgaa gcgcttgggc
    18001 taaacgagcc ggaagagtgg cggatcaccg cccccgtcag gcgcggctgc ctcatcgacg
    18061 cgctcatcgg catcacgcgt gcgacgcccg gccgcggcgg tcttcaattc gccgatgaag
    18121 acgcggttcg gatacagcgc cagaagacag tctttctcta tcgtcttgac gcgatccggc
    18181 ccgccgacta cgatcgagtt ctctccgctc ctgatgcgga tattttccag tttgagatcg
    18241 ctccatgaat gcactcaccc gcaggacgtt tcttggaagt tcgagcgctg cgctcgcctc
    18301 ggcaagtctc tcacaggcta taagtccccc cgccggatcg gcggatgtgc agggacagcc
    18361 ggtcccgatc gacttccgtt attcgcccct ttcgtggcag acggcgtact gcttccccga
    18421 cgaccctttc aagagcctcg tcggagaacg cggcgatctg cgctacgggc atcccggtcc
    18481 cggcaagccg atcgactact tcccggtcgt ggtcgaattc tcactcctcg gcgtcgaacc
    18541 cgacaccgcc cgccgccagt ggctcgatgc gccggacctg cccgttgtcc acacgctgat
    18601 caccaggccc gacggactcc tggaactcac cgccttcgcc acccacctgg ccggcgaagg
    18661 ccgcgtcgac aacatcatcc tggaggttcg gccccggagc ggggaagagg cgtatgtccg
    18721 ccccgtgatc accgtccgga cgagagagga actacaggtc aaggtcgaag acagcatcag
    18781 cctggtgttc ctcgcgacgc aaaccgagaa gccctttctc gtcgcggact gcccgctaga
    18841 ccgccgggac atcggaacag gctggatcct cacgcttccg gccgctgtct ccacccccgg
    18901 aaaacccgtc gtgcacctcc ttcggttccc gcaggagagc caaccggcgg agagactcgt
    18961 cgccggactg aaggacccca gccgcctcac gatggagaca cgggcgtatt ggacaggctg
    19021 gagccccttt ggcggcgacg tgcgatggaa actcgccggc cgtcaccacg agttccttct
    19081 cgcgtgcgct cgcaacatcc agcaggcgcg cgaagagcgc aacgggaaac tcaccttcca
    19141 ggtcggtcct accgtgtacc gcggtctgtg ggtcgtcgac ggccatttca tcctcgaagc
    19201 cgcgcgctac ctcggctacg acgccgaagc gcagcagggc ctggaggcga cctgggcgcg
    19261 gcaggacgct gaaggaggca tcttcgccgg cggcggccgc gagcattgga aggacaccgg
    19321 catcgccatg tttacactcg tccggcaggc cgagttgagc ggaaactggg actactttcg
    19381 cgcgatgcaa ccccagattc tgcgtggtgt ccgtttcctc gcggcgctgc gcgacaaggc
    19441 gaaagctgag ggcagcgcca acggccgtta cggtctgctc gcgcgcggtt tccccgacgg
    19501 cggcatcgcc ggcctgcgtt cggagttcac gaacaccctg tgggcgcttg ccggtctgaa
    19561 agccgtcgtg gaagcgtcgg aacggctgcg gatgtcaggc ttcgaagagg cgaaatcgct
    19621 ccacgacggg ctgcgcagcg ctctctccgc tgccatgcgc gaggagatgc gccgccacgc
    19681 gcgcggtttc gactacttgc cgatgctgat gaaggaagac ccgcagtggt cggccgaaaa
    19741 caagtggcag caaccccgcc cgcagagtgc gcagtgggcg ctctcgcagg ccatctaccc
    19801 cggcatcctc ttcgaaaaag acgacccggt cgtgcgcggt cacattgcgc tgatgcaagc
    19861 ctgcacgcag gaagacgtcc cggccgaaac cggctggatt cagcacggcg gcctctggaa
    19921 ctacggcgcc gccttcgtcg cccacgcgta cctttgggcg ggcctcacgg actgggcacg
    19981 gcttacattc accggtttcc tgaaccacgc gacgccgctt tactgctggc gcgaggaaca
    20041 accgcttcgc ggcgcaatgg ccgccggcta cgtcggcgac atgccgcaca attgggcgag
    20101 cgccgaatgt gtcctctatc tgcgacacat gctggcgctc gaggacggac cggccctgcg
    20161 gctgcttccc ggcatcggtg accccgagct ggccgcggcg gcgccctgca cgatcacggc
    20221 gtcgccgacg cggttcggcc gcgtgagcct ttctctggaa ccacttcccc gcgccgcagg
    20281 ctggcgcttg agtttccagc gtcaggacgg cccgactccg ggtgcagtcc agttgccggc
    20341 caccctcgga tcccggctca tgtcggcgga aatttcggga gcggcgtcga agcgcgacgg
    20401 cgcagcgatt tccgtggagc ccggcgcgcg ctcctggaca tgtgtctgga gggccgggtg
    20461 atacccgcta ccaataccac ttgagccgca ctcccaagct ccgcggttgc accagccgtg
    20521 tccccacgaa gatcgactgg aagttgtaaa gcgccgtccg gttcatcagg ttcgacacct
    20581 gcagaaccac ctcccaagtg cgacggtcat ctctgacgcg ctcgtagccg acagccatgt
    20641 ccaccagggt cctgggtctc acgcgcggcg gattggcgcc gagatcgacg tacggtagca
    20701 ggtccgcgta gtccggatcc gcggccacaa ctttcggatc cgacggattc gtgactaggc
    20761 cgctgtcata tcgagtggaa gccgtcgtcc agatgccctt atgcggccgg taagtgacga
    20821 cgccctgcac gccaagtttc tggtcgtgat cgatcacgaa cggtcctgaa cggaggagat
    20881 cgatcgccgt gtttcccaga aaaagcccgc ctgtgaacgg tggcgtcacc agcacgctgt
    20941 aatgcgtgaa gctcaccgac cccgaaaacc tccggaactc cggcactgcg atccttccct
    21001 ccgcgccgcg caccttcgcc ctcgccagcg ctgtcgggaa gatgatgcct gtgttgaaaa
    21061 agttgtcatt gtcgtgcaga ttgcggatgt ccttccggaa gtacatgaca ttgacgctga
    21121 ccttgccgaa cagcggctgc tggacgccgg cctcgtacac gttctgccgc tcgggcggaa
    21181 tcggaatgag cgccccgccg agcctcgccc gcacttcagg cggcaccagc acgctcgacc
    21241 gctcggaatt cgacagcagc agattctcgt tgaccggtgt ctgatagatg cgattgtacg
    21301 aggcgcggaa caccgtcccg gtcctcttca cattccaggc tgcgccaagc ctcggctgaa
    21361 actggccgcc ctccaccaag aacgcatacc ggtcatagcg gaacccgagg ttcagcagca
    21421 tcggcccaac gcgaaactta tcctgtacga atcccgaata gaggcctccc gacgcacggt
    21481 ccgaaaactg gaacaggcta ccgccgcggc tcaggtcgaa attctccaga gtggggatga
    21541 atcggtccga ccccggcgga ttgaacctcg catccgtaat gccgaagctg aagttctcgc
    21601 tcacggggaa gtgctggtag tcgaatccga agcgaagctc gtgagggcct tggatcaggc
    21661 tcatccggtt cgcgaccgtg acgttcgaga ggtgtcgcgc ctgcgccgct gttaccggag
    21721 tatcgcccgc gctcgggaaa agctgcgcca cggaagtgcg gtaggacgcc gtcgtgtcaa
    21781 gcgtggccga ggatccgatc acgtggatgt atcccagcga aatcgaggcg tcgcgcagca
    21841 attggcgctg gtcctggcca ctcacgtgct gcgatcgcaa gttcgcgagc tggaagcccg
    21901 accgggccac catcgcgttg agcctcagaa aatccgccgg cccgtgctgg taatcgagcc
    21961 gcgcaaaggc tcgctccgca tttccgccgt tatggaggtt gtcgatcgat acctggtcca
    22021 ggaaccggtt gctcttctgc gccgagatcg atgcgaagta gccgaaccgt tcggtttgcc
    22081 cgccgaactg cgtcacgttg gcggcatggt cgaactgagc cgcgctcact tccgtgctgc
    22141 cgaagaatcg ccgccccgaa ttcaaccctg accttgtcgt gatattcgcc acaccggaca
    22201 ccttcgaccc gtactcagcc ggaatgttcc ccgtgtgcaa ctcgatcgtc tgcacgatgg
    22261 cgctgtcaat gccgttccca aacgctcctg tgagctggtc gctgatcggc atcccgtcaa
    22321 tcacatacgt catctggttg tgtgccccgc gcggatggat cgctccgttg gcgtcggcgg
    22381 cgaagccggg gaagctcaac agcacggatt cgagcgcgcg gctgccgacg gggatggcca
    22441 tccgttcgat ctgcgccagg ttcaactggg tgcgcgtgcc cgtcgcctcc gtatccacta
    22501 attgcaccgt ctcaaaagcg ctcactgcaa tccgttccgc tacgtccgcc agttccagcc
    22561 ggatttccag ttccaccggc agattcgagc gcaaggctac ggtttgccgc tttacggcga
    22621 agccctcttt cgtcaccgtg agcacgtact cccggaatgg gatgttgctg atcgagaagg
    22681 cgccgtcctc cggcgtccgg gccgtctcag cgtacccggt gagtgcgttc tcaatcgtga
    22741 ccgcggctcc gccgacgttc gctcccgtcg ggtcctgcac ccgcccccgc aacgtcgctg
    22801 tcacctgcgc acccagagag agcggcaaca agataagaag aagaaggcag ccccgcatat
    22861 tcgttaatcg tagtttacca agaaatatga acctcagttt aaatcattcg tgaatggaat
    22921 gcaaccccgc agcgatacac tgggcaaatg tttcttggcc atcttgcggt cggctttgcg
    22981 gccaaacggc ttgctccgtc cacttcgctc ggaacactgt ttgcggcctg cgaatggatc
    23041 gacctcgtat ggccagcctt cctgctcgcc ggttgggagc aggtgcgggt gcatcccggc
    23101 gatacggcct tcaccccgct ggaattcgtc tcttacccgc tcacccacag ccttctgatg
    23161 gccgctgcat gggcggttgc cttcggcgtg gcgtacgcgc ttcgcacccg caagatgcgc
    23221 gctgctttgg tctgcggagg tgttgtgctg agccactggt ttctggattg gctcacgcac
    23281 cgccccgacc tgcccctgat tccgggtggc cacaagttcg gagcgggtct ctggaacttc
    23341 gttccggcga cactgaccct tgagctgacg atgttcaccg ccggagttgt gctctacacg
    23401 cgcatgacac gcgcccggga tcgtaagggc gccttcgggc tcatcgctct cgtaggtttt
    23461 cttgcggcgg tttatgccgc caacgtcttc ggcccgcccc cgccggatga gtccgcggtg
    23521 gcttgggcct ctctgtcgct gtggcttctc cccttctggg ccgcctgggt cgatcgccac
    23581 cgcgaatcgg cacgtcgagg cgtttcctcc gcgtttctca cgccgtcttg cgttcagccg
    23641 gctgagggcg tttcaaagcc ccctcgatat cggtcatctc gggccgccgg tgcagcgaga
    23701 agtagacgcc gagaaacacg acgactgcca ggccgaccag ggccatgaat gcaaggaagc
    23761 ggctcaccgt ccggacaggt gcgaatgctt caagggaatc ctggctcacc agcacaaccc
    23821 agccgagatt ccggtagtcg gccttgagtc cggtgtccgc gaacccaatc agttttcggg
    23881 ccccgccact cacgtccgcc accatgtagc ccgtctgacg gccgtgcagc gtttgcagcg
    23941 catcctttac ggtggcatag atgtcggact tcaggttcat cgagaacaca acgcctggag
    24001 cagcgataat tgttccgtcc tccttgacca gcgccgcgcg cgatgtgggg ccgagttgca
    24061 cacggttcac gaacgggaac aggctcgtga cgtccaccag ggcgtccacc gccccgacaa
    24121 acctctgggt accctcctcg acgatcggaa ccccgatgcc gatgtaattc gacttggtga
    24181 cctcgtcgta cagaatgtct gtgaggttga cggtgccacg gcccttcgcg tagatggcct
    24241 gccagaagtc ctcgtccgcc tgaaagtaat cgagtgtctt gtgcgttgct gcgactgtgg
    24301 cgccgcgagc gtccgtgatg gtgacgcgaa ggaaccgcgg atcgagttcc ctgtggtgca
    24361 gcaaggttcg cgacgcacgc gaggtaagca tcccgcgcac ggtggcctca gcggcgggcg
    24421 tgttccactc gttttcaatg cgctgtatcc gtccggctat ggcggcttcg ctcatcccct
    24481 ggtaggcaag gtttgcggcg gtgacagcgt cgacgacggt tggctcgttc gccatcacgc
    24541 tgacgttgac cactcgttcg tggatgtatg cggcaatttc cgtcgcggtt ccttcggcga
    24601 tcgtccggaa atgggtgccg atggtattga cgagagctct ctcgctttgc gagatggtcc
    24661 aaagaccggc aaggcagatc ggaacaatcg tcagcagcag tccgatgagt agccgtttga
    24721 ccggtacacg aatttcgtat ctttctgaag gcatggtatg actcccagga actcgggtct
    24781 gaaactgcgg tcgcgcgctg cgttgcgggc gtgccggaag ggttgggaac acccttgcgc
    24841 gatacgctca ggagtgccat cgagaggccg catgacctcc ctccgcttac gagcataaac
    24901 ccaaatcggt gcggtgaata gccccgaatc cgaaaacgaa ggtctttggt aatctggcga
    24961 aatatcgtgg agtactggaa gtactgcgac aaatgccgcc ggttccccga aaggcgctga
    25021 aaacacccga agaaccccat tccggtgcga ccacttgacg ctcatgcgca tcactgttgt
    25081 attgacacgc ccttgaattg gtagtactaa gtgagaagcg gcagaggagg agtccgcacc
    25141 ggtgcgggcc ggggcgggag gagttatttc gtgcagagtg gttcaaacgg caaagaagaa
    25201 ttcgtcgact attacgaatt gatgcagatc agcccgaacg ctgagcacga gaccatacag
    25261 cgtgtctacc gtatgctcgc ggcccgtcac catcccgaca accccgagac gggcgacgcg
    25321 gagcggttcc ttcgcctcaa cgaggcatac cggacgctca gcgaccatga acgccgcgct
    25381 gcttacgacc ttgtctacca cacgcgccag gccgagccgc tccgggtttt tgagctgaag
    25441 gaattcgcgg cgggaatcga cggcgaggcc aaccggcgga tgggaatcct ctgcctccta
    25501 tacagccgga ggcgcaccaa tcccgagcat cccggagtct ccatcttgga gctcgaaacg
    25561 atgatgtggt tcccgcgcga gcatctcgtg tttaccgtct ggtatctgaa ggagaagaac
    25621 tacatccgcc aggacgatca cagcaacctc atgatcacgg cggaaggcgc ggactatgtc
    25681 gaggagaatc tgccctcgaa caagactctt tacaaactgc tcaaggcctc cgaactcggc
    25741 gccacccgct cggaacgcga cgtcaactgg cacgcggacg atcagccata tcctacaaga
    25801 gcagagaact cgtgacgccc gatcgttcca tcctgcaacc gaagtgcccg gcgaaacacg
    25861 ccgcctgaag gagaaagagg atacgccatg cgatcgacga tagtgacccc gctgcttctt
    25921 ttcgcggtcc ttgccgcgtc ggctaccgcc gccgcccaga ccgtcgtcga gcattccctc
    25981 accaccggag ccatgggaac cgcggccgcc ggcggagcca agggcgcttc gaagtcgatc
    26041 ggcagcgtat tcggcaatgt cagcaagacg ctcgacaaag cggccggttc aggcgccgcc
    26101 gccaccagcg cggccaccgc caccgagcca gcctcctcga cttcctcgac ctcggagacg
    26161 atcatcacgt cgaaacctcc cagtccccgc gattcggccc cgcagaaacc tgccgatcct
    26221 ttcaaaatac gggtcggcat ggctcgcgtc acgctggtcg aaacggctgg aaagccgatg
    26281 atgaagacaa gccgggtgga ggggacgtcc tttgtcgaga cctactacta tcaggggctg
    26341 gatgacgtgg tggtagtcac tctgcgcgag ggcaaagtga cggacgtcat cccaccgccg
    26401 cccacggacg cgaaagagag tgcccgccgc tagcggtcct aggtcaccag ccggcggcgc
    26461 ttcgcttttc gcgacgagat tccaccagga gttccaggct ggcaagcgcc agccccgcga
    26521 gaattcccag gtgcagcgcg cccatcgcac cgaagacggt ttcgagcgcc cctcccgtag
    26581 tcggatacag tcccatgccg cgggaaactg cgaacgccac cgcggcaatc agtcccgcac
    26641 tcgccgccgt tactccagca cgcccgaagc gacgcgcgct ttttgacgct acgcggtccc
    26701 acggcaggag tccggtcagc acggtcgcga gcccggcggc aacgaaagcg agaaacgacg
    26761 ccgacagtgg caggtacggt tgcgccgctg cctcctccgc ctcggccggt aactgcgccc
    26821 acaaacccca cccgagaatc gccgccgaca tcagtacgcc ttcgcggcgg aaacgcctcg
    26881 cgaacacagc cagcgcaagg aatggcaaag ccagtacaac agccgtcacg aattgtcgca
    26941 tgccctcgct cgcgcccccg ttatgcgccc actaccgccg gatacacctt ctggatggct
    27001 gcgatgacgt cgtcgcaatc cctctttgta aacttcgggt ccatcgtcac ccctccgaag
    27061 cggttcagga tgtcgatagt gcgcgggcac gatgccgcgc cgtattggat cgcgcgtccc
    27121 cgttcggtgt tgaaagaggg ccagttcgga tggaccgtaa tcttcttctc gatgtgcggc
    27181 tggattggaa gtatgaccga ccctcccggc ggggaggctg gcacgttctc ggccttcatc
    27241 gccgccagga accgttcccg ttgctccttc gtcttgaatt cgatgaaaac actggatccg
    27301 agttcgccgt ccgggtcggg cagcttgcgg agacggaggt tcggaagatc gcggataccg
    27361 tcgtagaccc gtctcgcgtt gccgcgtacc gcgttcacga tcgtgtccag cttgcggacc
    27421 tgcgcgagca gcaccccgcc cgtgaactcg ttgaccctgt aacccgagcc gatgaaccag
    27481 tccagccgct tgccgccaag ccagtcctcg tggacatttc gcaagccgcc gaggtcgtga
    27541 taccgcgccg cccgttcgaa caactccggg ttgtttgata caaccgcgcc gccctctccc
    27601 gcggtgatcg tcttgttgag ctgcaagcta tagatggaga tgtccccgag agagccgagc
    27661 ggtttccctt tgtagctcgc tccgacgctc tgcgcgcagt cttcgagcac gagaatccga
    27721 tgcttgcggg cgaccgccat cacccgatcc atgtcgcacg ggttcccttg aaggtggacc
    27781 gccatcaccg ctttcgtttg tggcgtgatt ttgcgctcaa aatcctccgg gtcgatgttg
    27841 agcgtctcgt cgatctcggc gatcaccggc aaggcccccg ccagaaccac tgcgttgaaa
    27901 cacgagtgcc aggtccacgc cggaacgatt acctcgtcgc ccggaccgat acccatcccg
    27961 gccaccgcgc attgcagcgc ggcggtgccg gacgtcaccg caagggcgta cttggtctgc
    28021 atccgggccg cgaactcctt ctcgaaggtc atcaccttct tcggcggctc gttgcccggg
    28081 ccgtaccacc ggaacggcct ccgggtctcc agcacctcga gcagttcctg cttctccttg
    28141 tcgtcgtagt attgcgttcc atagaacccg gcgcggagcg gcttctcacg caccggggcg
    28201 ccgccgtcca ccgccagctt gggcgaggac gaagcggttc cggcagcggc cgctaccgcc
    28261 agaaacgttc tgcgattgac catggctgac tccttttcct caacagcata gtccccccgg
    28321 gcgtccggca caacggttat cgtacggtgg ccggatcgat ttcagtaatt tcttgtccgt
    28381 aataaccgaa gttatggtgc gatgtcaacc ttcgtcagta ttgctccatt acgcctgtga
    28441 gcatacaatc agcctacctg caggaatacc aggacaaggc cggtacaaca acagcgatag
    28501 tcaggaggaa gtgaacgtga acagaacatt accagggcag attgcccgag tttgcatcct
    28561 gccggcgctc ttgctcgtcc ccgcattgct gaccgggcag cgtccctacc cttgtgtgga
    28621 tagcccgaag atctttttgt atccggattt cgcggggcaa gtctacgttg tgccagacaa
    28681 cctctgcttc agcaacggtc ccggaatcga ggtccaggcc gacggcgtca ccattgacct
    28741 agcaggtaag acgctcttct gctccggctc accgctaggg tgccaaggta actctttggc
    28801 agtcggcatt gacgtcggct ctcacaagaa cgtcaccatc aaagacacca tcggtctcgg
    28861 tagaatagaa ggtttccata caggtatcct agtggataag ggtgatcggg tccgcatcga
    28921 gtcgttgacg atcacgggca ctcatgcaaa cttcgatgcc tttcgtggcg acgccacggg
    28981 gatactcgtg aaggatgttg catgccccgg ggctattctg atcgatggta acgatgtgag
    29041 ctggcaccgc tacggcattc ggctactgaa ttcatcttgc gttaagatac agcgcaatat
    29101 cgtccaccac aacaacaaca atggcggcga ggcctatggc atcctactat ccggtttgtc
    29161 cgatccgccg tccgttatgt tcgggtcgag cggcaacaca atcacgaaca acacgctaac
    29221 gtacaacggg ttccataata cttacggctc atccggggag attgacgccg gcttggtgct
    29281 agatggtcgg gacaccaccg gcaaccgagt cagccagaac accgttctat ccaattgtgg
    29341 agatggcatt atgaccagac agtcggcgag cggcaacacc gtcagcaaca acacagttca
    29401 tgacaacccg acgcaaagca atggacccga acaaagctgt agctggccct ctcccactca
    29461 gcttaggaac tatgatcttg cttcacgtgg ggccggcgaa aaccgctgga gcaaaaccaa
    29521 cgactgcggg gtatccttcg gcattctccc gggcgtctgc atcgtcagca aataaaatct
    29581 ccctctcgat cgctgtgaca ccagccttgt tcacgcaggg cggcactcgg ccgccctgcg
    29641 tatttcccgt ttttccagca gcttgcatgt gagcggccga tggtacgcgc gcgcttcttt
    29701 tcctagcctg ctatccgaag atatgggaac gtcgtgtccc tttttcagta ttgcctccgc
    29761 ttcatttgac ccctacaatc gaagcaatcc gtgacgatta cggacaaatg gagccggtaa
    29821 tgaagctcat tcttttggta ctcatacctc tgatggtctt tgcccattcg gaaagtgacc
    29881 cgctttgcgg aaccgccatc tatgaggacc tcgtgctcga ccacgacgtc acctgctaca
    29941 actccaacgg gctgattatc ggcgccgagg gcctcacgat tgatctgaac ggcaagaaga
    30001 tcacctgcaa caacggcgag tccatgggtt gcacggggcg aggcttgacg ggaatcgacg
    30061 cgtccaaagt ccaagccgtc tcgatcaaag gtccgggcac agttcttggc ttccatctga
    30121 gcatgcgcgt tgccggcacc ccgcaactct cgcgcgcggt ctctgcccgc cgtccagtca
    30181 gcgcttcgcg ccgggctccg ctcaaaacca gagtcgactg acgcgcactc ccatttccct
    30241 accaaaactc cggtggtaga ttaaagctgg agactaccgc tgggaggtgt cacgatggat
    30301 gccgctgcga agctacagcc ggagctggac acggaggagt tggggctgct ggtggaactt
    30361 ctcaatcgcg agctgcgcga tttgcccgcg gagatccacc atacccgtac cgctgctttc
    30421 cgggagcaat tgcgccgtcg aatggccgcc gcggaacggc taatggctcg tttgaggccg
    30481 ttgttgcggg aatagcgctc ccgcagggcg aacgaaagac gcgccaggcc tcgcgagaga
    30541 ccgtccgagg atgttctggc ctcttcaggc cgccatgttc tcgacgatag cggtggcgaa
    30601 ttcactggtc ttcagcttcg tcgccccccc catcagccgg tggaggtcat aggtcactcg
    30661 cttctgccgg atcgtctccg tgatcccctt ctcgatgagc tgcgacacct cttgccagcc
    30721 catgaactcg aacatcatcg agccggaaag gatcaagctg gaggggttga tcacatcctg
    30781 gcccgcgtac tttggagcgg tcccgtgagt cgcctcgaac acgccgtgct cgtcgccgat
    30841 gttggcgccc ggagccatcc ccaatcctcc cacctgggct gcgcaagcgt ccgaaaggta
    30901 gtccccattc aggttcggcg ttgcgagcac ttcgtattcg tccggacgaa gcagcacttg
    30961 ctggaacatc gaatcggcta tgcggtcctt cacgagtagc tttccggcag gaacaccact
    31021 tccggcctcc gcctcggtga tgcaggcatc tgcaaactct tctttcacaa gctcgtagcc
    31081 ccagtcgcgg aatgccccct cggtgaactt catgatattg cctttgtgta ccagggtgac
    31141 gctcttcttg ccggttcgca gcgcgtactg aattgccctc cggatcaatc gcttggagcc
    31201 gcccacgctc attggcttca cacccacacc ggaatcgggg cggatcttgg tccccatctc
    31261 gctgttgagg aagtcaatta ctttcttcgc ctcaggcgta ccctggcgcc actcgatgcc
    31321 ggcatagacg tcatccgtgt tctcccggaa gatcaccaca ttcagcttct cggggttctt
    31381 catcggcgac gggacgcccg ggtagtagcg gactggccgc atgcacacgt agagggtcaa
    31441 tagttgccgc agcgagacgt tcaggctgcg gattccgccg ccaaccgggg tcgtaagcgg
    31501 ccccttgatg gcgatgtgaa aacgctggat cgccgccagg gtgtcctcgg gaagccagct
    31561 attgaagcgg ttgaacgcct tctcgccggc gaaaatctcg taccactcca cccgccgccg
    31621 tcccccgtac gccagcttta ccgccgcatc aaatacggtc ttggaagccc tccagatgtc
    31681 agggccggtg ccatctcctt caataaaggg cagaatcggt ttgtctggga caatgaactt
    31741 gccgtctttg acttcgattt gcgctccgcc ggcaggaaca ggaactccat tgtacgaatc
    31801 catggtctct ccaagtggtc tacttacgat aacaagccgg cttgcggctg acaatatttt
    31861 ccgtctggta caaatcagat attgagatct ggaacgaatt gtgataaagc agaggcaggg
    31921 tgaggcgccc tccattcggc actgtttctg gcgcactgcc tcacccgtgc aacaggtttt
    31981 ttaacagcta gcggctccgc ggatgagacg actcctgtgc aaggagctaa ctccatggaa
    32041 aatgagacct taacgatcat tgacaaccga actcagaagt cctataccgt tcccctacac
    32101 cggggcaacg tgcgcgcgat cgagcttcgg caaatcaaga aggatgccga agacttcggg
    32161 gtgatgagct acgacccagc tttcaccaac accgcctcct gccagagcgc aatcaccttc
    32221 atcgacggcg acaaaggcat cctgcgctat cgcgggtacc agattcagga tctggccgag
    32281 cactgctcgt tcctcgaagt ggcctacctg atccttttcg gggaactccc taccgaatcc
    32341 gagctcaaag agtggaccgg caagatcacc caccacacga tgatccacga gaacatcaag
    32401 aagttcatgg atgggtttca ccacgatgcc catcccatgg gcatgcttgt cagcacagtg
    32461 gcggcgctct caaccttcta tcccgatgcc cggagcattc acgacaggca atcgcgaaag
    32521 ctccagattc tccggctgat cggcaagatg cccaccattg ccgcttacgc ctatcggcac
    32581 agccttggcc tcccctacgt atatccggac aactccctca cctacaccga gaacttcatg
    32641 aacatgctct ggaagatggc cgagctgaag tacatgccga atccggcgct ttcccgagct
    32701 cttgacatcc tgttcatcct gcacgccgac cacgagcaga attgcagcgc caacgcgatg
    32761 cgctccgtcg gaagctcgca agctgacccg tttgtatcca ccgccgctgc cgccgcggct
    32821 ctatccggac cactccatgg aggggccaac gaagaagtcc ttaagatgct cgacgagatc
    32881 ggctccgtgg acaacgtccc cgcgtacgtc gaccggatca aggccggcca tggcaagctg
    32941 atgggctttg gccaccgcat ctacaagaac tacgacccgc gggccaacat catcaagaag
    33001 gcagccgaag cagtcttcaa ggttaccggc cgcaatccga aactcgacat cgcgatcgag
    33061 ctcgaaaaga tcgccgtcca ggacgagtac ttcgtaaaac gcaagctgta tcccaacgtc
    33121 gacttctact cgggcatcat ctatcaggcg ctgggcttta cgcccgatat gttcacggtg
    33181 ctgtttgcca tcccgcgtac cgtgggctgg ctagcccagt ggcaggagat gcttgaagat
    33241 ccggagcaga aaatcgcgcg gccgcgccag gattataccg gccacatgct gcgcgagttt
    33301 gttcccgccg cacagcgcgt cgcgacaaca gcggctgtga ccgtttgagc gagaccggat
    33361 tcaggccgcc gcctgcccgt tgccgccgcc ggtgggagtg aaccgtgcca caacgaccgt
    33421 caggtcgtct tggggcgcgg cggaaaccga gtgctcccgg acggcgcgca agacggcggc
    33481 ctgaatctcg gagacaggtt cgctgaagtg ggcgctcact ttcgtgagca ccctctcttc
    33541 tccgaattct tcgtcctgtt ggttggcagt ctcgacgacc ccatccgaat acagcacgag
    33601 caggtcgcct cgctgaacgg ttagctttcc ctcctcgaat ggcgcgtgcg gcaacaaccc
    33661 tagcaccggt ccgcccgtct ccagacggtc cgatagcacc ggaccgttgt cccctcgtct
    33721 caacagcaac ggtgggttgt gccccgcatt gacgtaacga agcgtcgtcg agtcccgttc
    33781 gaacacaccc acgaacatgc tggcaaagac ttcgcgggag gtctgcgagt acaggaagcc
    33841 gttgaggtgc gtcaccatct ccgacagcga ggacgttgca gcgatcgccg atgccgttcg
    33901 gaccgcgccg tggagaagtc ccatcaaaag cgcggcaggc agccctttcc ccgacacgtc
    33961 gccgagaacc agcacggtcc gcccgccgga atctggatag acgtcgtaaa aatcgccacc
    34021 tacctcccac gccggctggc attcggctgc gaactcgatc tcctcacacc cagggcatcc
    34081 ccgcggtagc agttcctgct ggacctggcg ggcaatcgag agctgctgct cgatatcgcg
    34141 cgatcgcaga tagccgcgga atcgtacccc catcacgacc atcgacgcaa ccagcgcgat
    34201 cgccgccgcc gtcccgacca ccaggttccg gcgtagtggg aggaacgtgc tggaagcacc
    34261 ctcgaggtaa agcgccacct cgattgcccg gaaccggggt tcacccggcc ggggtggctg
    34321 gtttccccta aacagcgggc gttccgcggg gaagcggaac cgaaacggca tcgctaccac
    34381 gacgactggt cccttcgggg tgtcgcgggt ctggcggatg tttcgctcca tcccagtcag
    34441 gacggactga aggtagggga taggaagtgt cgtaaggtcg gcgccacggc tctccccgac
    34501 aaccgcacca tgccggtcat attcccgcaa ccacgcaatc tgctcggggt gctcgctgat
    34561 caattcgtcg aacaccgacc gtaatgtgtc gaaggagtcg atggaccggt cccgggcccg
    34621 gttctccagt agcgaagcat agtgccccgc ttcgtcggtt agctcgtcga ggaccagcgt
    34681 tcctgtgacg tgccggtaag taagaatcgt ttccaccagc agcaggattc ccagtacgaa
    34741 tccgcacacc aaggccgcct ggaacaaaat ggccctgctg cgcttcgaat cgaaagtatt
    34801 gatcgagggc actcgctcct tagtctatcc tgacacggaa gtccgaagaa gcgttcgcgc
    34861 attggcgatt gcgtgggcgt gaaagtcgtt gttgaaatac gcgtagacga tccgccctgc
    34921 cgcgagccag tcgcgtacgc gccgggccca cggctccagg tccgcattcg aatacgccga
    34981 cgcgaagagt tggccggggc cgtgaaaccg gaggtagacg aagtctgccg tcggaacgca
    35041 tggggcctgc ggatagcgct cggagttcgc gatcacaagc cccgctcgcg attgtctcaa
    35101 cgcctcgagg atctctgggt cgaaccagct cgcgtggcga aactcaaacg ccagtttcac
    35161 ctccggtaga ggacgcctca tcttcagaaa ccggctcagc gccctgaggt cggctcgcga
    35221 cgatggcggg agctggagaa ggatcggacc aagcttcttc ccgagcgcgg cagcgttttc
    35281 gaggaacgta ctccactcct tctcaattcc cttcagacgc ttgatgtgcg tgatgtagcg
    35341 gcttgccttg acggcgaaca caaacccgtc cggcgtctgt tccgcccact tgcgatacgt
    35401 cgcgggccgg ggcaggtggt agaaggagta gttcacttcg acagtgtcaa accctccggc
    35461 gtagtaccgt aggtactcgc tgctggctac ttcctcggga tagaaacgtc cgcgccagtc
    35521 gctgtagttc caacccgacg tgccaacgcg aagctccggc ggagtgtcca tgtcagccgc
    35581 ctcgctgtgg cgattataga tgaaagcagt acagtcggcc tggatctcgg cgtccgtcac
    35641 tagctctgga cgtttgcccg gagcagccga agcgcgttga agataacgag cagcgaaacg
    35701 cccatgtccg cggtgatggc cgcccaaagt gatgcgtagc cggcgaatgt caagagcacg
    35761 aacaccgctt tcacgagcag tgaggccaca acgttctgcc ggatgaccgt caacgttcgc
    35821 ctggagtgag caagaagcca cggcaccttt gacagatcgt cggacatgag cgcgacatcc
    35881 gccgcctcaa tcgcggcgtc attgccggcc gcgcccatcg cgatccccag gcttgatctt
    35941 gccatcgccg gtgcgtcgtt gatcccgtct cccaccatcg cggtcgggcc gtaacgcacc
    36001 acgagttcct caatggcggc gaccttgtct tccggcagca gttcggcgcg aaactccgta
    36061 atgccggcat ccgtggcaac ggccgccgcc gtccgctcgt tatcccccgt aagcatcacc
    36121 acgtgcctga ttccggcctt gtataagcgt tggactacgt cgcggacgcc cggccttatt
    36181 tcatcggcga atgcgatgag tccgatcacg cgcgcgtcgt ctccggcaaa gacgatcgtg
    36241 cggccgagcc cgtacagccg ttcctcatgc tcgccgatct cagccgtctt cagcgcgcgt
    36301 tcggccgcat gccgatggga accgagccag aacgagcggc ccccgatccg tgccgtaatc
    36361 cctttgccgg gcaggacctg aacgtcggtc gcctccgccg gcgggacctc gcgaattttc
    36421 gcatagctta ccagcgcctg tgccagcgga tgatccgtgc gcgattcaat gccggcggcg
    36481 atctggagca gccgggcttc gctgtacccg tccagagcta cgacctccac gacaaccggc
    36541 ctgcctctcg tgagcgtccc cgtcttgtca atcgccacgg cctggagatg cgcgggcgcc
    36601 tccacgtaaa tgcccccttt gatgagaact ccggcgcggg cagccgtggc cagagcggcc
    36661 acgatactca ccggcgtcga gattaccagc gcacacggac aggcaatcac cagcaggacg
    36721 aggctccggt agatccactc gtgccacggt gcgttcagca gcatcggcgg aatcacgagc
    36781 acgccggctg cgagcgccat gacacccggc gtgtagtact cggcgaatcg gttgacccac
    36841 tgctctgagg ccgctcgccg gtgttgcgcc tgttcgatca ggcggatgat gtgggcgagc
    36901 acggtgtccg atgccggctt cgtggagcgg acctcgaggg cgccgttgcc gttcacagtg
    36961 cccgcgaata cggcagcgcc cggttctttc gcgacgggca cgctctcacc ggtgatggaa
    37021 gcctggttga cttcgctcgt gccttcgacc acctgcccgt ctaacggaat gcggtcaccc
    37081 gggttcacca agaaacggga gccggttggc acctcctcgg cccgtacctg ccgctccccg
    37141 ccgccgggta tctgaatgcg ggcgaggggc ggggcgagat ccagcagcgc ctccaccgcg
    37201 cgccgcgccc gggccacgct ccatgactcc agcgccagcg agaggccgaa caggaatgag
    37261 accgtggcag cttcgaacca ttccccgatc gcgatagcgc cgaacacggc gaccgtcatc
    37321 agcaggttca tatcgggccg gcgccggcgc aatgccgccc aggccttcgg gaacacgtac
    37381 cagccgcccg cgacgatggc aatcgtgtac aacgtccggg cagcaagcga aggcgcccca
    37441 cccgctgcgg tcccttcggg acgcaggatc tccagaatgc cgcccgcaag ccacgcatga
    37501 acggcgaagc cggccaaaac cgcgagtccg ctcgctgccg tcagagccgt gcggccccgg
    37561 gtttcccaga agctgcgccg cgtgacgtcc gattcacttc gccacggctc cgcacgcatt
    37621 cccgtccggc taacggcagc cacaatcgcg tccggtgagg tgaccgcgtc ttcaacgatc
    37681 atgatgccct tcagcaggtc gaacgagagc cgatcttcgc cgccggcaac cggaccgatc
    37741 tcgcggcgaa ggatcgcgat ctcatcagcg cagtccatcc cccgaattcg aaaacgaagc
    37801 tgggaagctc tcacgaagcc tcagcccccc tccgatcgat tctataagat gaattcagtg
    37861 gcatcatgca gaattcgatg aaatggggga ctgatttcat tccgctgctg ttgcgaaatg
    37921 gcggtgtgcc cccatttcat cccttccgat gcctgtccgc actcatcctt tccgccctcc
    37981 tgctcagcgc gcccgcctgc gcccagacta gcggcgatga cgaccacttc cgccgcggct
    38041 ccgatctctt cgtccggcag aaacccatgg aagccgccgt cgagttcgaa caatcgctcc
    38101 agcgcgaccc ccgccagccg cgcgccgcaa aactgctcgg cctctgctac caccttctca
    38161 aggacaatga caaagccgag aaggcgttcc tcctggccgt caaactggac ccgggggacg
    38221 ccgacgccca cttctttctc ggccggcttc actacctcaa gaacttcttc gagaaggcgc
    38281 gcaaggaatt ggatgttgcg ctcaagctga atcctcgcga cccccgcgcg cacagttacc
    38341 tcgccctgac tctcgaagcc atgggcgata acgacaaggc cctcgcggag caccaggcgg
    38401 ccgtgaagtg gaacgataaa ctgccgaaac ccgatttccg cccccacgag agctacggcg
    38461 ccttcctctt gaagctcaac cgcctccccg agagcgagca acaactccgc cgtgcgaagg
    38521 aaatcgaccc ttctatttgg gagacgctct tcgagctggg caaactgtac caccggaagg
    38581 gcgatctgga agcctcgcgg ctgcatctcg aagccgcttt gaaggcgggc tccgcggatc
    38641 cggaggacac ggcgcgaatc cggcatttgc tcgcgcgcgt ctactacggc ctcggacgcc
    38701 gcgaggacgc tgcgaagctg atcggcgtgg agatccccac cccatgaatc gccgcctcgg
    38761 actctctgga atcgccgtgg cggcggcgtt ccttatgcta agtggggctg agaaaacggt
    38821 cgtgcctgtg gtcttcgaat cggccggtgc gaaatccggc ctcgacttcg tcctcggcaa
    38881 ccatgccacc cccgacaagc accaggtcga gacgatgccc gccggtgtcg gcattatcga
    38941 cttcgacaac gacgggttcg aggacgtctt cttcatcaac ggcgccacgg tgcccggcct
    39001 cacaaagaaa gaccggacct gttggaacag gctctatcgg aacaacgggg atggcacatt
    39061 ccgcgatgtg accgaaaagg ccggcctctc tggcgaaggc tacagcatgg cggtcgccat
    39121 cggcgattat gacaacgacg gctggagcga cctcttcatc gccggcgtca accggaacat
    39181 cctctaccga aacaacggtg atggcacgtt caccgatgtc acacggaagg cgggcgtcgc
    39241 cggcgacaac ccgcaaaaaa tgtggtccat ctccgccggt tggttcgact acaacaacga
    39301 cggcctgctc gacctgtttg tcgtcaacta ctgcaagtgg gaccccctaa ccgagccgta
    39361 ctgcggcctc cgcaagcccg gctaccgcac ctactgtcat ccgaagcact acgagggcct
    39421 gccgagcacc ttataccgga acaacgggga cggggcgttc accgacgtct ccgccgagtc
    39481 aggtatcgcc gcgcacatcg ggaaaggcat gggggtggcc ttcgccgatt acgacgacga
    39541 cggccggacc gatgtattcg tctgcaatga caccgttcgg aatttcctgt ttcacaacga
    39601 cggaaacggt aagttcaccg aggtcggcgt ctgggcgggc gtctccctga cggaggacgg
    39661 ggcgccgatc tcctccatgg gcgtcgattt ccgtgacatc aacaacgacg gccggcccga
    39721 tctcttcgtc agcgctcttt cgaatgagac gttccctttg ttccttaaca tgggcaaagg
    39781 cgcattccgc gacattacct atgccagcca gattgggttt ctcagcttgc cgtcaggagg
    39841 gttctctgcg ggagtcttcg acttgaacaa cgacggctgg aaagatatct ttgtcgccgg
    39901 ctcccacgtc atggacaacg aggagctgta ttcgagccgc gcttcccgcc aaccgaaccg
    39961 cgtcttcgta aatcttggcg gcgaccggtt cgccgatggt aaggatggat cgcccgcgga
    40021 tgtctggaaa ccccgctttc accgcggctg cgccttcgct gatttcgaca acgacggccg
    40081 catcgacgtc gtcgtgtcgg cgctgaacga acccgccgag ttgctacgca acgcttcacg
    40141 ccccgaacag tactggcttc agcttctgct gcgcggtgta cgaagcaatc gcgacgcgat
    40201 cggtgccaag atccggatcg tctcggagtc gcgcgccgag cagcacaacc acgtcacaac
    40261 atccgtggga tacgcgtcct ccagcacgag gcgcgtccac tttggcctcg gcaacgactc
    40321 gctcgtgaaa cggatcgaaa tccgctggcc ctccggtaaa acgcaggcgc tcgagaatat
    40381 caaagcccgc caattgctcc gcctcaccga accagagtga aaacctgtcc cctggttttc
    40441 cttccgctca gtacactggt agccatgact ttgactcgac gcaccctctt cggcgccctc
    40501 agtgcctcgg ccgcccttgc ccagcggcgg cagtttgcca actggaagcc gaagctcggt
    40561 attctctgcc ggtattcaaa atccaatatt gagtttgcga aaggagacgg ctttgccagc
    40621 ctccaactga acgtcggcgg gcagctcccg cacgacgtcg gcgacaacgt aatcgccgaa
    40681 gtgaagggcc agatcgatcg gagtggactc tatgtgtcat ctctgatgat ggtggtgaat
    40741 cacattgatc ccgatcccgc caagagtgcc gccggcaaca gcgactttgc gaagtgcatc
    40801 gaactggccg gaaagatgcg tatcccgtat gtggcgacca tgtccggcaa aaacgccaac
    40861 atcggtttcg acaaacagat cgatgagatc gtccgcgtgt acaacgagaa gtacttcccg
    40921 ctctgtcaga agcacaacgt caagatcatt tgggagccat ggccggaagg accgaacgtc
    40981 gccaccggac cggaaggcta tgaagcgctg ttcaccgcct tcaaagactc cccctacgtc
    41041 ggtctgctct atgacccgtc acacctcgtg cgccagttca tggatcccat gcagtgcgcc
    41101 cgcgacttcg tcgacaagat ctgggacgtg catttgaagg acgtcgagat tatgtggcac
    41161 gtcctgcgca aggttggcat ccagcccttc aggaacgtga gctggtggcg ctaccgcctg
    41221 ccaggtcacg gacaactgaa ctgggcggaa ttcttcacga ttctccagga agccggctac
    41281 aaaggcgcga tgaacatcga gcacgaggat gcattctact atccggccta cgaaggcgac
    41341 gatttcacgc ctcagtacaa ggagggtttc cgcgtgacgc ataagttcct ccgccagttc
    41401 gtccccgcgt aacacaagtg gcgcaggtct cccgacctgg gaaggcaagc tcctgacctg
    41461 cgaccaccgt gcgcgcgttc ggagcgctac ttacaggtta agtgtcttac aggttaagtg
    41521 ccttgacacc acccgctctt tccggctata agggatacag tcctatgtgc cctctgtggg
    41581 ctgggattgg ggtcagttct ggaagtggca agcccacacg gcctggatta ggaggaaact
    41641 cggatgatcc tggtcagatt catcctcgtg ttcgcttttc tcgccccggg cttctgcttc
    41701 gctcaggcca cctacaccgc ccagttgcga ggaaccatca ctgacagcac ggccgctgtc
    41761 attcccagcg ccaaggtaac cgttaccgat gacgccaccg gcgtatccga aaccatttcg
    41821 tcggatggac agggacggta tctatttgcg tccctgaggc ccgcctccta taccgtgaaa
    41881 gttgaactaa ccggcttcaa gactgtcgtg cggccgaatg tcgtcctgcg tgtcagccag
    41941 cagacggacc tcgacttcac gctcgaggtg ggccagacta ccgagacagt ggaagtcacc
    42001 gccgaatctc cgcttctgaa caccgtgacg gcgaccttgg gaacggaggt caccaatcgg
    42061 tacatcatga acatgccgct tctggacagg gcgctcacaa gcctcacctt cctcgcgccc
    42121 ggagtgacgg aagttcccgg cgccggggcc gatgatattc gtggcaccaa cttcgtgtcc
    42181 aacggccagc ggaacgggac ggccgaagtc cgtctggacg gagcgctggc gacagtgccg
    42241 gaaagtgggg aaggtgggaa caccatcgtc aactaccagc cgtcggtcga gatcatccag
    42301 gagttcaaag ttcagaacaa caactactcc gccgaatacg gaaacaacgg cggcacggtg
    42361 gtcaacatcg tcacaaagtc cgggaccaac cagtttcacg ggagtggctg gtggttcttc
    42421 cggcggccgc gttttgacgc caacgacttc ttctccaacc gggaagggca gccgaaggga
    42481 gattacgaga aagacgaatg gggaggctcg attggcggcc ccatcaagaa gcagaaggcg
    42541 ttcttcttct tcgacttctt gaagaccagg gacaattcgc ccttcacgct gaccacaacc
    42601 gtgccaaccg cgctccagaa ggcgggtgat ttctcccaga cctttaatga cgacggctct
    42661 ctccagacca tcttcaatcc ccgcgacgtc catgaagatg aaaacgggaa ctggatccgc
    42721 gcgccgttcc cggagaacag gatcccgacg gactggatcg acccggtcgc gcgaaagata
    42781 gtccaactct atcccgaagc gacaggctcg ggcgacgacg tgacggggcg caacaactac
    42841 accaaaaagc tggtgagttc gtacccgggt tatcagctcg acacgaagat cgatttcgtc
    42901 ccgaccgcca agacccgcat cggtggtcgc tacagccgcg gccattctag tggctcaact
    42961 ccttcggcgg acggccagtt gctcgacgag gcaaccagca ccggaaacaa tcaaaatgcc
    43021 gtgctggagc acaattggac tcccacgccg acgattctgt ggaccaaccg ggttgggatc
    43081 gatcgcagct tctcccacca gattgcaagc gagcgggatc cggcgagcgt gggcttcccg
    43141 ccggaactca acggctactt cggtctgaaa cgctttccgg agatcggccc agaagagtac
    43201 ggctacctcg ggcaatcctg ctgtgtcgac actgtgaagg gacagacgca gtggatgttt
    43261 tcctcctcgt tgtccaaagt tatcggcggt cacaatctca agttcggtgg ggagcagcgc
    43321 cactcgttca ccaacttctg gcagcccgac tatcccaccg gccagttcgc cttcgagcgt
    43381 tccaatacga tgcaggacgt gttcaacccc gactacatgc aaggcaacgg tcttgcctca
    43441 ctgctggtcg gatggggtaa ctacgcgcac gtcggaacgc agccgcctgt tgcggataag
    43501 tccaaggaca cggggttctt catccaggac gactggagag tcacccagcg cctgaccctt
    43561 aacttcggtc ttcggtacga gtggagcagc ccgttcacgg agcggttcaa ccgtcttgtc
    43621 gtggtggact acaatggcga cacgggtgta gacgtgccgg agctggggcg gctgaaaggc
    43681 accagctacc tggccgactc caagaagcgg acggcgaacg tggacaggaa caacttcggt
    43741 ccgcggttcg ggtttgcctt ccgcctcaac gagaataccg tcgtacgtgg cggggcgggt
    43801 ctctactacg gatataacgt ggctactaac ttccagtatg tcggcactcc ctggtataag
    43861 aacgtgcaag tttatttctc caaggatggc ggcattacgc agtacgcaac gctgtccaat
    43921 ccgtttcccg tcggctttgt gggcccgccg ggaccgaagt acgggaagct cgcgcaatgg
    43981 ggattcgaca acggctacaa cctcaacgac gggcttcgga acgccgagat ctaccagtgg
    44041 aacatcggca tagaacgcaa gttgccgtcc gacatgttga ttgagatcaa ctacacggcg
    44101 aaccggagca cgcatcttcc gttcaacggt tacgacggca cgcggaacag gaatttcatc
    44161 aatcgggaga accgcgagcg gtacggaagc gacgggctgg cggaactggt tgacaatccg
    44221 ttctatggct tcttccaggg acccgacgcc atcttcaacg agcccgactc tccctacaac
    44281 gatcctcaga ttgagcgcat ctatctctta cggccatacc cgcagttcgc cggcagtttc
    44341 ggcgggtatc cccagttcat cgccaacgca aggtataacg ccttgcagat caggttcgag
    44401 aagcgatact cgcacgggtt ggccttcacg ggtaattaca ccttctccag aatgaccgac
    44461 gataattcgc tcgggttcaa tccgtgggtg ggtaacctgc aaaccaccgg cgagcttcag
    44521 gatctgaccg acctcagtac cgagaaatcg agaagtggcg tggatacgcc acagcggctg
    44581 gcgttcgccg ttagctacga cctcccggtg gggcgaggca agttggtggg cggcaagatg
    44641 ggcaggggtc tcgacgccgt ggtgggaggc tggaaaacca acgccttcgt caccttccaa
    44701 tccggcaacc ctgtgggcgt gcacatggct gagggtcgat tggcggacgg caatcagcgc
    44761 cccaatgtct cgggaagcgt ctccggtgcc ggcgtccagg cggtagtgga cggtgcgggg
    44821 aacttcttca acgtcgacgc cttttcggac ccgggcgacc aggtcccagg caatgcgccg
    44881 cgttacttct ctgacctgcg gacccacggg atcaagaaca tcgacttctc cctcttcaag
    44941 gagttctact tccgggagga cgtctacctc caattgaggg ccgagttctt caacttcctg
    45001 aatacgccga ggtttgggtt acctgacact tcatacggga gcagcgattt cggtctgatc
    45061 acgtaccagg caaaccgcgc gcggaacggc cagattgcga tacgcttcgt gtggtagaag
    45121 acagggcgcc ggccgcgccg cggtcctcgc gttctgcgcg ctgtcgtgcg ctctgcacac
    45181 ccttctggcc gcaccgcaga aaccgacggc tgaagatcac acagcgaagg gtttggcaga
    45241 tgcccgaaac ggcgatctga agagcgccga atccgagctt cgaaaggcct tggcgctgtc
    45301 tccggatgat gccttttgcc tgggaaccct gggcgccatc ctggggatgc aaaacaagct
    45361 ggaagagtcg agtcagttct tacaaaaagc actgaaggtc aacccggagg actggacaac
    45421 acggcgcaat ctcgcttcga accagtttca gctcggcagg ctagccgagg ccagggagaa
    45481 tctcgacatc gtcctgaaga agaatcccga agacaagacg gccgttttgc tccttgggat
    45541 ggtggccgag gaaatggggg actacgccac cgcagtgggg cgcttgacct cagtgcccga
    45601 gctgaccgcg tggaacccga agtcaattgc cgcgctggca cgttcgtatt accggaccgg
    45661 agcgcacgag caggcccgag ctactttgcg gcaaatgatc gattccgaag gagtgctcct
    45721 gggagcccgg atcgcagccg gagctgacga tttccagcag gcggagagca tgcttgcctc
    45781 cctgccgcct tccttccaac acggaaacga cacgcgttac gagttggcgc tggttcgata
    45841 tcgcgccggt aaatacgcgg aaagcgagaa gacgctgagg ggtttgttgg atagcggcgt
    45901 gcagaacagc gacgttttca acctgcttgc ctggtgtcat tacaaacagg accggatgaa
    45961 ggaagccgtg gcggcgatgg acctggcgat cgaccgcgat ccagcgggcg aatccaacta
    46021 ttacgacctt ggcctgatgc tgcttagtcg tgaccgcgcc cccgtcgctc tggaggcagc
    46081 caggaaggcc gttgaggttg cttcgggttc ctaccgtgct cacctgctga agggcctcgt
    46141 ggaggccaaa ctgaaccgcc tggacgacgc cgccatttcc tatgcgcgcg caagcgagtt
    46201 gaaaccggat gcgccggagg cggtcgtggc gctgggcgtc gtactttccg ccgctcgaag
    46261 gtatcccgaa gcggagaaga ccttccgcag cgggctggag aaatttcctc gctgtgccgc
    46321 gttgtaccag gagtatggaa agtcgctcct ggagcaaagc gacttcgcgg acgccgtcgg
    46381 gcaggcccgc gctgtccgcc tgctcgagac ggcactgtct ctggaccccg ctctttccga
    46441 gccgcatttc cagcttggca aactggcgct ggaccacgat cggccgcggg aggctgcctc
    46501 acacctcgag atcgcagcca agctgaaccc ggcaagcagc cggattcact tcgtgctttc
    46561 gcgtgcctat actgagttgc agcgtccgga caacgcggcg cgcgaaatgg agctgttcga
    46621 gcaggcgaaa gcacgcgagg agggctcggc gccgacggcc tcgcgcgggg aaacgcctcc
    46681 gctgccgcaa tagcgcagat gatcttctga agtggcgcag gtctcccgac ctgcgatgca
    46741 ctgagaatcg gcgacgaaag gaacttccga aatgaagacg aacacttgga ccttggcggc
    46801 actcattctt tcggcatcgt ggtgcttcgg agcggaacag aactggactc atttcgtacg
    46861 gatcggaggc cacggccttg cgcttggccg cgccggcgcc attgtccgga gcgccgtcgg
    46921 gaccaacgtg tacggtatcg aagtggacaa cgacatcacc ggccgttacg agagccttct
    46981 ggacccggcc gcgaagctgc gtgcgattcg cgagcttgcc gacgccgcgc acgccgccgg
    47041 ccagaaagcg ttcgtctaca tcgcgggaac tgagtgcatt accgccaacg ccgacaaggc
    47101 tcctcggtcg atgttcaaag atcatcccga ttgggtgcag cgaaagatca ccggcgagcc
    47161 ggcggtgttc accggcggtg cggccttctg gatcaggaag ggcgacgagg acgtctggat
    47221 cagcccatac gcgtccgaat ggcgcaagac gtacatggag cgcgtgcggc agattgccgc
    47281 aaccggaatt gatggggtgt acgtagacat cccgtactgg atgacacact tcgacgggtg
    47341 ggaggattcc tgggcgagtt ttgacgacca tacagtggcc gccttcaaag cgaaaaccgg
    47401 cctcgatgca aagaaggaca tcaaggtggg cgacttcgcc gaccctggct tccgtaagtg
    47461 ggtcgacttc cgcatcacca cgctgacgga gttcatgcgg gaaatcgacg agaacgtgaa
    47521 gaaagtgaat ccacagtgta tgaccatccc cgagatctac ccggggatcg aagcggaggc
    47581 cgtcagggtc ggttcagacg tctatgagat gtaccgcgtc accgacgcga tcgcgcacga
    47641 gtacgagttt gacggcggcg cggcgatagg tgcttccaag acgccgctcg actggctcca
    47701 ctacatgacc ggcatgtaca ccttccgcgc gttcgccgaa ggaaaggcct cctggatgct
    47761 gagttactcc tgggatggag agaagagcgt cgatccgaag gaggcgatga aaaacctctt
    47821 cgccgcgcag ttgacggccg gaacgaacag ttgggacgcg gctacgcacg taatgtccgg
    47881 ctcgaatgac atggccacgc ggaaggcgat cttcggatgg atcgccgccc acgaaaagat
    47941 cttctatcgc cctcgaatcc ccttgaggcc gatcggcgtc tatttctcgc cggcgacgcg
    48001 taactacttt gctcgcgaat ggatggaatc ctacctgggg acgatggcga tgttgctgca
    48061 atcgcaccgc gagttcgaaa tcgtgacgcc gcgaaccctg ccggctttcc gcggcgaagt
    48121 gctcgtgctg ccggatgcgc ggtgcctgag cgagaaggag atgcagcgtc tcgaaaagtt
    48181 cgccgggcag ggcaaggctc tgattctcac cggcgagacg ggcaagctgg acgagcgcgc
    48241 ggaagcgcgt ccgggtaacg cggtgacgcg actggtccgt tcggccggga acgccgctgc
    48301 tccgcgtgtt gtagcgatgc cgaaatgtcc cggccgggcg tactgggcat tgctagggaa
    48361 acaatatgat cgtgcggcgg cctccgggga tccgctgccg gacacaacgc aagccacgat
    48421 ccttcgcgac actcttcagg agttcaaata ccaaccggcg gtggagatcg ctgcctcgcc
    48481 ctttgtcacg gcgcagatcg ccaaggtgga tggcaaggtc cacgtgttcc tggctaattt
    48541 caagggactg aagagtaagg agaatgccgt gcagataccg gaaaaaggcg tgcgtatctc
    48601 gttcccggcg gatgccggac aacgcgtaca tttcttgccc tatcttgatg aggcgagcga
    48661 gatccgatcg gaacggaagg acggcaggct gagttgtatc cttcccgaca tccacaaagc
    48721 cgccgtcgtc tgggtggact gagatatcgt tactgagcct caaccgcagg gaggggtctt
    48781 tctttcctcc tgcgcgttct ccgtgtcttc tgtggtgaat cgcttttaga catttacgga
    48841 agccgtgacc gtgagcggta gttcaggttt tcatgatgga ccattgtcta ccgactggga
    48901 caagccttac tgtgtcctga gtgcagtttg cgaacagaca gagctgcctg tctcgtctcc
    48961 tcataaactg ggcagtcttg gctcacgggc cgagggggcg gcagcgagaa aacccgggac
    49021 tgactccaat ttcgctgcct tttgcgaaat tggtgtctgt accgaggttt tctcactctc
    49081 tacctttcgt ggagtgggcc gttaggcccg ccaccccggc agtcctgccg gtggtcggct
    49141 tcacaggtgg cacaggcttc agccaggctt cagcctgtgc gcatgcagtt tccgagcggc
    49201 gcccggggca agttcctcga agcgccaggg tgcacgcatg atgcccgcaa gtggagaact
    49261 tccgataaac cggctgctgc cggtggagaa ccttgaccag ccggaacggg ttggaggatt
    49321 gaccgcgact ggcaggtaag tacaatcggt ggtggccagg aaacccgatg aagaaagaag
    49381 acgaccgcct cggcaggact acccaaagta gccccgaaca gccgctctcc gccaccgtgc
    49441 cacacacgcc ggccaacgat cctttcatgt ctcgccgcga cgttctcgct gcgtcgatag
    49501 cgctgggctc gccgctcctc ttgaatcact gtggcgtcgg acgggagaag gtgtacgaca
    49561 cggtcatccg gaacgggcgg gtgatggatc cggaatccgg tgtggacgcg gtgaccggcg
    49621 tcggcatctc agatggcaag gtgttggccg tgtcgaagac ggcactcgag gggcgcacga
    49681 cccttgacgc aacgggactg gtggtggcgc ccggcttcat cgacgtccac cagcacgggc
    49741 aggacctgga gaattaccgt ctcaaagtga tggacggcgt gaccacagcc ctggaactgg
    49801 aactgggcac ccacgacgtc gaccgctggt acgccgaacg ggaagggaag gcgccgatcc
    49861 acttcggcgt gagcgccggg caccccgcgg cgcgaatgga ggtgttgggc gagcctctca
    49921 aattcctgcc ggccggcgac ggcgctcacc gcgccgcgac ggacgcggaa atcgcgcaaa
    49981 ttcgcggccg aattgagcac gggttgcggc gaggggcgct cgcggtgggc atggggatcc
    50041 agtacactcc ggcagcatcg cgatgggaga ttctggagat gttccgcgcc gctgcacggt
    50101 acggagcact gtgtcacgtt catctgcgga acatggggga gcgcgaaccg ggaagcagcg
    50161 tcgagggcct cgaagaagcg ctggcagccg caaccatcac tggtgcgccg ctgcacgtgg
    50221 ttcacattac gagcagcggt ctaagggctg cggacaagct gctcgcgatg atcggcgagg
    50281 cgcgcaagcg gggaatgaac gtgacgacgg agtgttatcc gtacagcgcg gcgatgacgg
    50341 cgctcgagtc ggcgctcttc gacgagggct ggcagcagcg gctggggatc gggtacggag
    50401 aagtggagtg ggcggcgacg ggcgagcggc tgacggccgc gaccttcgcg gcgtaccgga
    50461 agaggggcgg cctcgcgatc atccacatga tccccgaaaa tgtggtggcg ctggcagtag
    50521 caagtccgct gacaatgatc gcaagcgacg gcttgattca aggcggcaag ggccacccgc
    50581 gcgtttcagg aacgttttcg agggtgctgg gccggtacgt gcgcgaatct cggtccctga
    50641 gcctgatggg tgcgctgcgg aagatgacgc tcgatccggc gcgctccttg gagcggcgcg
    50701 tgccttcgat gagagacaag ggacggattc gtgtgggggc cggcgccgat ctcaccgttt
    50761 tcgacccggc gcaagtcctc gaccaggcga cgtacgccga gccggcgaaa ccatcggcag
    50821 ggattaggca cgttctcgtt agcggacagg ccgtggtgaa ggacggcaaa ctcgtggatg
    50881 ggatgctgcc gggccgcgcc gttcgcgcgc cggtagacca ggcctcgtag cgctcgacgg
    50941 ccagagtgct gttaccctgg agtcagagcg caaaggagcg ctgtgaccaa gaaattcttg
    51001 atgttcctgg ggactctcgc cggcgccttg cctctgtccg gcggccagat cttcggaaca
    51061 ctgaccgagg gagggaaagc cgtcgccaaa ggagtggaag tggtagtcga ttgcgccggc
    51121 gaacaggtaa agggcacgac ggacaacttc ggaggatatc gcgtgaacgt ccagaaaacg
    51181 ggccgatgta cgtttacgct gacttacaaa ggccagaagc cggcggccgc gatcgcgtcg
    51241 ctccgtgaac ccgtttcggc ggactttgag ctgcggatgc aggatgggaa gtacaaactc
    51301 gtccggcggt gacgtccatg gccacggaac cacaaccggc tcggccgaag gaacccccac
    51361 ccccaaggga ctggtggcga tggttgcaga ttctcgtcat ccccgtgacg accatcatcg
    51421 tcggccagta cgcctcgaac tggcaggaaa aggagagcgc gcggcgaacc tatgccgaca
    51481 tgatgaaccg tcgggcggaa gcggaaagcg ctcttcggaa ggatatgttc cagacgacga
    51541 tcgtgccgtt tctggagagg aagaccgatg acatggaggc cagcgtgctg cgtctggaac
    51601 tgctggccag caacttccac gactccctgg atctgagccc gttgttccgt gacgtttacc
    51661 ggacgttggc cgccgagcga aagcagggct acgaggaact gatgatgcgg ctaacgacct
    51721 cggcccaaga ggtcatacgc cgggaggtgg acgccatccg cgaagcttcc accattcgga
    51781 ccggcacggt ggacgtcgag agcctcaagc agggtccgct gcctttgttt ggagaagagg
    51841 ccctgaatct tccggggacc agttcgccgc cgcgcacgtt ccgcgtcgag gtcctcgact
    51901 ttgacaagca gcggagcgaa ttcctggttc ggctcacagt gtggaacgcg ggtagcccgc
    51961 cggtgatgga actgcaaacg aaggcgttcc atcttgggcc tttcgacttc ccgctcatcg
    52021 acaacacgcg actggctgga ggcgaccggg tggcgttgac cttggacgga gcggccgaag
    52081 atgtggccca ggtcatgctg atctacttcc cgggttctcg cgccagcatg aaggacaaac
    52141 agtactacga agatatggct gccgagctcc tgcgcattga ggagcggaac cgtgagaagt
    52201 ctcaactttc caggtgagtg ctgagctcga cggttgtgcc aggatcagac cacaggtaga
    52261 ctatatagta tctgggttca tctggcgatc accggctcac gcccctggtt ccggactctt
    52321 ttctcggtgt cctactcatg ggtgctgaag taggctgggc gggggctcca aggcacaacc
    52381 gggagcttcg agtgacatga ccaaagcgga cgttttcccg gcttggggcc gcatcctctc
    52441 gggacaccgg ccccttttgg cgctcgagat caccagggag tgccctttgc gatgtcccgg
    52501 ttgctacgcg tacgaattgg gacatctccc gaatcaggtt cccctgcgcg agctgagcga
    52561 ccttcgaggc gacgatctgg taaatggtgt gctcgctctg gttcgccgct ttcggcccat
    52621 ccacatctcg atcgtgggag gggaaccgtt ggtccggtat cgggagttgg acgttctgct
    52681 gcccaagttg gctgccatga ggatcgaagt gcagctcgtc acgagcgcgg tgcggccgat
    52741 tcccgcggct tgggcggagt tgccgaatct tcacctggtc gtctcgattg acggcttgca
    52801 gccggagcac gaccgcaggc gatctcccgc cacctatgct cgtattctcg aaaacatttc
    52861 cagccatcgg atcattgtgc actgcacggt tacgaggaag ctcctcgttc gcccggggta
    52921 tctccgcgat ttcgtcggct tctggtcgga acggcaggag gttcggaaaa tctggtttag
    52981 cctcttcact ccacaggagg gcgactattc ggaagagcgg ctgacctcca aggatcgctc
    53041 ggatgtggtg gcggaactga gccgcttgag agctttcccc aaagtggata tgccggaggt
    53101 ggttctcgat ggctacctgc acccgccgga gtcgccgcgg cagtgcgcct ttgcgcagtt
    53161 gacgacctgc atctccgccg acttggccac ccgcattacc ccctgccagt tcggcgggcg
    53221 gcccgtctgc tccgagtgtg gctgcatggc ctcggccggc ttggctgcga ttgcgaaata
    53281 taagctgggc ggcttgatcc cggtctcgag cattctttcc ctctccagga ggaccggcga
    53341 gcagctatcc cagatgggct tcgtctgatc agtaggctgc cgggggccgc aagtgcaggg
    53401 aagaaatggg gactgactac catttcgccg ccgttgcgaa atggctgtct gtctcccatt
    53461 tcttcttgcg ttcagtcgcg ctaattcgcg cctcctattc cacccaccag ctcgtaatag
    53521 cacgccgccg gctgcgcggc cgtggggcag acgatcgctt tttgtcgtct gccaccctcg
    53581 caggccacga tgtccagaat attgccccgt ccataaatag tggacaacag aaatatttgg
    53641 acaggaacgg gacaacacca tattccccca acatccgttt gcgtttatct gtggcccctc
    53701 ctactccacc accagctcgt aatagcacgc cgccggctgc gcggccaccc gctgccgcgt
    53761 ctccaggtcc gcttccagcg cgtcgaggtg gtagcacgcc gaaaacgtgg tacctgcaat
    53821 accactcccc ctgaggttgg gttgatgccg cggcgctatt gcccgtggga gatgagagtg
    53881 agccccggaa ccccgaggca atcatttcgg ttatgggtga tcagcggcgc gccggtgatc
    53941 agggcagtgg ccgcgatcca ggcatcggcg cagtcaatcc tccgcccgct cgcctgtgct
    54001 gcgacggtga cctccgccca cttcgtgcag agatcccggt cgtacggcag cacgacaaaa
    54061 ggctccagat actctcgaag ccggttccgg cgcatagacg cttcacaaga acagcgaaaa
    54121 acccgccgaa tacgccaggt tgtgctgcca gcccggttcc ggcgttatct ggatatcccg
    54181 cagcttctgg atcacgtaat cgcgaacgtc caaccggatg ccatagcggt cgctgagccg
    54241 tatcttcagt ccgcctccga cattggctac gaacttacga tcgtggcgga tcgtcgcgcc
    54301 acccagcccc gcggttgcaa acgggcggac tcgacttagc tggcgggcgg catgcgccac
    54361 gaagttgtag cagagcgtgt gaacccccaa accctctccc gccacttccc gattcgtgat
    54421 ctccagcgga ggattcttcg gcgaaacggt gtacttcaga tgagtgcgct gataagagta
    54481 accgacctcg tgcgcgaggt attcccattg gttgatagtg atggcagccc cagcccggaa
    54541 gccgtcgctc agccgcacgt cgagcaggac gggctgcgga tacgcgagca cagtcgtctc
    54601 gcccagcttt ccgccacgga acgtggacca gcccgcctgg acagccactt ccatctgcgc
    54661 atgggaagct tgcgctgcca gcagaacgcc ggcgagcagg caaaccgggc gaaccacctg
    54721 ggagacgcca cgcttacgga acatagcggc ctcctttcat cggggcgaga aattctgtcc
    54781 cttggacgcg ctggaatttc ccgcggttgc gctctccttg cccgccgcca cgacgaccag
    54841 agccaagcaa agtcagggaa gtgccatatt tcccacgttc atagtgcgtc cgcggtacgg
    54901 ttcgttacag ggcgcgtgtc gggttcaagc gcccttggac tgcccgcggc ccctattccg
    54961 ccaccagttc gtaatagcac gccgccggct gagcagttgc ccgctgccgc gcctccaggt
    55021 ccgcgtccag cgcgtcgagg ttctccagaa tcgaccctac gcgcggcagg ttgtcctccg
    55081 cgagcggtac ctcgacctgc cgccaccggg tctggtgtac cccggaccgc ttcagtgtca
    55141 actcgtgcac gctcgccgcc tgcgctgcca tcggcgtcgg caactcggcc aggttgatcc
    55201 ccctgccaag ctgcgcggcg tcaaacgagc ccactgtcgc gccgttgatc ttcagcgtgt
    55261 accgcgcggc cttcaacccg gaaaccttta gcgtctgctg attaagcgtc tcgacgaaat
    55321 ccgacgaccg caccgccagc gcaatcaccg cgtctttcat gttgaccggc atcggcagcg
    55381 cggcgtcctt ctgcgtccac gaaaccgtct tgcctgtcgc gacgcccgtg acctccgtat
    55441 tcttcgcctc tagaaccctg cccgccgccg cgtcgatctc cacggccgtc accagcggcc
    55501 ccgctcccca cgccttcagc agacaggccg ccatcaacag atggccgccc ggacccggat
    55561 gcacgcggtc cgggatgatt cgctgcgctt gtttcggatc ggtggcgttc gctttgcgca
    55621 acgcctcgac caccggcccg ttcaggtccg ccaccgccat cccattgcgc tgcgccagtt
    55681 ctttcacaaa ctcgccgtag cgaaccagca ccgcgttgta gccgccgggg aacgtcgggt
    55741 cgcgcgtgac gtcgtcgaac ggcgacggct ggatcagcgt catccgaatg ccgggcgccg
    55801 cttccttcat catcttcacg atgtgctcga agccggccgt gtactcccgg aaaatctcct
    55861 cgtcaaacgc gcggtaccgg ccgtcgttca tgccgagcat caccgtcaac gccgtcggct
    55921 tgtacgcaac cacatcccgc tccagccgct tgtcgatcgg cccgccgcct cctccggaca
    55981 cgcggtcgcc accccatccg gagtgaacga accgaaccgg cagcttcgga tagcgcgtca
    56041 ccacgaacgt ctcgacgaac gtcgtgtaca gccgctgatc ggtgatgctg tcaccgtaga
    56101 acacgaccgt gtcattcggc cgcaacgaaa agccgtcttg cgccggcatg acgcagacga
    56161 acgcaacagc gaggctcagc aggaatcgta aacgcataat ctgctcatgg tacttcgaaa
    56221 cttcggacaa tcggtcgtcc tgtgatataa aagggtcctt ataggcgaag accgaatgca
    56281 aaaatccaaa ctgctgactg cgattccttt gctcgtcgtg ctcgtcggcg cggggtgtgg
    56341 cgggagttcc tcgctgcgca cgtggcacgt ggattccctc gtcaaggtgt tccccgacga
    56401 caagccgggc acgaacgagt tgtcagacaa gacatggctt gttgcgcgca acggccacgt
    56461 taacgttcag gtggccctcc gttcggaaaa ggcgatcccg caactgcgag tcgaactcaa
    56521 agcacccgag aacgacggca agaaacttga ggcggaagcg cgctgggtcg agtacgtgcc
    56581 catcgggtcc aacccgcccg gcacgccgtt cgacgagttg gttcgtcctg ctcccggctt
    56641 atttcccgat ccgctgatgg agacgtggcc gttcgcgctg aaggcggcca tcacgcagcc
    56701 ggtgtgggtt acggtccacg ccccggcgga cgcgcagccc ggcgaataca aaggcgaagt
    56761 aacgttcttc gccggacagg aaaggctgaa gtcggtggac ttcaccgtgc gcgttacagc
    56821 ggcggccgtt cccgcggagc agaagctcaa ggtcaccaac tggttcaatt tcagctacga
    56881 tcccgcgacc cccgatctga ggttgcacta caagatcgac ccttacagcg acaagcattg
    56941 ggagctgctc ggcaacctcg gcagggtgat ggcggcgcac aagcagaacg tcatcctcac
    57001 gccggtcatg gcgctggcga agccgtcggt cgccggcggc gccattcgct acgacttctc
    57061 gctgttcgac aagtgggtcg agacgttcca gaaggccggc ctcgtgggca ccatcgaggg
    57121 cgggcatctg ctcggccgca gcggcggctt cttcaccccg atggtcgtac ccgcctacgt
    57181 gatcgaaggc ggtcggattg tgcagaaggg actcgatacg gatgacccgc gcgcggagca
    57241 gttcctcggc tcgttcctca ccgcgctcta cgcgcacttg aaggagaaag gttgggagaa
    57301 gcagtacatc cagcacgtcc acgacgagcc gcatgaccgc gaagccgcga tctataaccg
    57361 gtactcgaag atcatccaca aatacctgcc tggcgtcccg accatcgacg ccgtcggcct
    57421 cgatcaggac atcagctttt tcgccgacgt ctgcgacatc tgggtcccgg tgctcagcag
    57481 cttcgaccac cagctcgaca agatgcgcgc tcacgtcgag aaaggcggcc aggcgtggtt
    57541 ctacacctgc atcggtccgc agggccggta cctcaaccgc ttcaccgacc tgccgctcgt
    57601 caagacccag cttctgcact ggttcaactt ccgctatgac ctcaccggct tcctccattg
    57661 gggcggcagt tcctgggggc cgaaaccctt ccagaacgtg cagacggtga tcaacgacaa
    57721 ccgcacgctg ctgccggcgg gcgacaacgc catcgtgtat ccgaacgccg agaagctctc
    57781 cgtgctcagc tcgatccgcc tcgaagcgat gcgtgagggc atcgaggact acgagctgct
    57841 ggtggcgctg gcgggcaagg atcaggcgaa ggcgaagggg ctggcgaaga ccgccatccc
    57901 caacatcaac gattacgtcc gcgacgtgcc ggcgttccgg aagctccaac ggcagcttct
    57961 ggaggcgttc taacgagggg aaaaaggtga tgaaaagagc cacttttggt gcacttttgg
    58021 tcgtttttgg actcattttg acactgtttt ggggctgttc ggcgggcccc gggctggaat
    58081 tttggttcgt ggacgcgctg gtaaaggtgt ttccggacga tccggcgggc actgcgagat
    58141 tggacaaggc ggtgttcccg gcggcgcgga attcaaacgt tagcattcaa ctggcgctgc
    58201 gggcgaagca gcccgttggc gacctgtacg tcgatgcgct ggcgctgacc ggccccgggc
    58261 aacccattga aacggcaagg gttcgttggg tggaatacgt ggtggtaacg accaacacgc
    58321 agaacacgcc ggaggaggag ctgctgcgaa aagcgccggc gttgtttccg gacgcgctct
    58381 tgacggagtt tccgatcacg gtgaagaagg accagacgcg ctcggtttgg gtgacgatcc
    58441 acgtcccggc gagccaggag cccggcgagt acaagggcga gctgcggctg cggcagggga
    58501 cggaggcgat cgcgaccgtt ccgtacacgc tggtggtcca caaggccacg gtcgcaacgc
    58561 cgattccgct ggcagtcaac aactacctca acctgagtga ctatcacctg gagcagtttc
    58621 atagctgcac gcgcggatcg gggccgtggt ggaacctgat cgcaaactac gcgcgtttct
    58681 gggccggcta ctaccagacg tcgatcggcg cgaatgcggt gtcgctcgcg aaaccggagc
    58741 tggcgggcgg agggctgagg tacaacttca gcgacttcga acgctacgtc gagacatggg
    58801 aggctgccgg agtgaagggc cagatcgacg gcggcaacct catggaacgg cagcgccggc
    58861 gggacgccac catcatgact gaagcctggg tggtcgaggg cggtaagccg gtcttgcgca
    58921 gcatcccctg gaacgaccca cgcgcgcaac agttcctcaa tacgttcctg ccggcgctct
    58981 acaaacggct ccaggagctc ggatggacga agaagtacgt ccagggaatc atggatgagc
    59041 cgaaccagtg ggagatcccg gcgttcctcg atgcggcggc caaggtgcgg aagttcatgc
    59101 cgggcgtgcg gacgattgag ccggtcgggc tgcggcagga tgtcggtttc atgaaaaaga
    59161 cggtcgacat ctgggtgccg ctgctaggca cgttcgacga gaagctggat ctattgcaga
    59221 agcacatcag cgaagacaac ggcgagatct ggtattacac ctgcctgtcg ccgcggggga
    59281 agtatcccaa ccggttcgtc gatacgtcac tgacgaaagt gcggcttctg cactggatca
    59341 actacaagtg gaacttcac
//
LOCUS       DATIFQ010000044        17731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_970635, whole genome shotgun sequence.
ACCESSION   DATIFQ010000044 DATIFQ010000000
VERSION     DATIFQ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17731
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970635"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VLE22_02060"
     CDS             <1..798
                     /locus_tag="VLE22_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB13212.1"
                     /translation="MLNIVGRGMAGRTTVEQDTTDMDPERTASEAARYPGLVVGIKIA
                     HYAGPEWIAVERAVEAGRRADLPVMVDFAEFRPERPFQDLVVKKLRPGDIYTHLYLGA
                     VPMLDPQGRVLPYLFEARKRGVIFDVGHGAGSFLFRQAVPAVRQGFVPDSISTDLHVG
                     SMNAGMKDMLNVMSKFLNMGMSLEDVILLSTWNPAREIRHTELGHLSVGSSADIAVLR
                     VVDGNFGFVDSSRRRLQGTKKLVAELTLRDGRVVWDLNGLTSDDWTK"
     gene            complement(808..2037)
                     /locus_tag="VLE22_02065"
     CDS             complement(808..2037)
                     /locus_tag="VLE22_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13213.1"
                     /translation="MKRRYALVALAFAVAAVGPTPARDAFRPVPLESRITNVQPMTGI
                     VMWEDSKNSETDAIQLEYSYMRYSDVVKQRGEYDWTPVERKLDASARRKHQAILRFWD
                     TYPGRPSSVPQYIKALPDYTDRVAKSERRDTGFPDWSHLEWQRLFLEFYEKFAEKYDR
                     DPRLAFLEVGFGLWSEYHVYSGPEVLDETFPSMEFQARFFHHLTKVLQHTPWMISQDA
                     HVAKRTPFASQPQLLKLRFGIFDDTFHLAWSPSYNLEGWEFFGRDRYKHSPMGGEILF
                     PGKERAEHVAEAWSRETRNFGITFMICEQWPRWTTMERIREHGLACGYKFKVASFEAG
                     AKASRVAVANTGVAPIYYDAFVAVNGVRAKESLKYLQPGETRQFTVASGGKNPSLTIE
                     CDRLVAGQRIEFEAGLK"
     gene            complement(2039..3613)
                     /locus_tag="VLE22_02070"
     CDS             complement(2039..3613)
                     /locus_tag="VLE22_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13214.1"
                     /translation="MIRRDFLRAAAITTLAPGITPALRADVPDHRWDGYDFGPAPAAT
                     DRLNQGPFGIDQDEGWQTIGFTNPSRGHVKNFGTGLVAYTWEEGGPSLAARRGQEPLE
                     EAVEKLASLPFVDVLYIRCDWRDVQKQPGKLDLNPVWKLTLDAAKRHNLRVGFRVQLS
                     SPNFQPKQLALPDFLQHKIPMVKIGRARGEGGGKPQDWDYVEPRYDHSEFQKAFQELN
                     ELLAAEFDGHPLLEFMDMMMYGFWGEGHTNRLPNPFPDYLTAEKTMAAMTRLQIDTWK
                     KTPLAINTQPDISRTGNRECLDIAVRAGCWLRSDSTVYIEEPIQVECLSNRPQWLAVV
                     MEEGRNRHYDPAALVFDAAGINDRENAALHVLDLCGNYWALWTEAENLQRFYEKYPFA
                     FETLHRRLGYRVRPSFIWQRKRYGTSEIILGIANDGVAGVPGVLRVHVESMDGRVRVG
                     GGLDGGHPHGGRLRQASFILPTGLEGQQVKLRAELETKGVRHPVRWTCAQPLHPDGSY
                     VVQLKQFDDRGWRKGV"
     gene            3808..5475
                     /locus_tag="VLE22_02075"
     CDS             3808..5475
                     /locus_tag="VLE22_02075"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSB13215.1"
                     /translation="MKTLSGVLCLLVIGFPARVGAAPDDRMASGSRGLVVSGRSAATA
                     AGIRILERDGNAADAAAATLLTLSVTTVGAFCIGGEVPLLVYDAKKNDVKVLSGQGGA
                     PLDPAAIEWYLKNGIPSNDVRAAAVPAVVDLVVTLLKLYGTKSFGEVVGPTLSILDAG
                     GPTWYIDTSDRRKIETGRNWRAELAVTFRKLVEAEQKAKGWRERRLQAVADRFYRGDI
                     ADALEKWYIAQGGFLRKADLAAHRTLVEEPVQVSYRGYAVLKCGPWTQGPYLLQSLRL
                     LEGFDLKGMGHASADYIHVVTEAMKLALADRDEYYGDPRFTMVPLTSLLSDPYTQMRR
                     PLIDIKKASLEVRPGDPYRMRPVKEPPPPAAASQGGTTVMCVADRWGNVIAATPSGLG
                     SSAGVAGDTGIIHGARLVSLNTWKGHPNCIAPGKRPRITLTPTLVLKNGKPLVAIAVA
                     GGDLQDQVSLQLILDHIDFGMVAESAFQAPRFATRHHTGSFGQDRPHLGSLQVSAKVS
                     ESVREDLKRRGHLVTTSSGEIGGAAMLSVDPASHEVRGAGPSAGRVE"
     gene            complement(5627..6505)
                     /locus_tag="VLE22_02080"
     CDS             complement(5627..6505)
                     /locus_tag="VLE22_02080"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB13216.1"
                     /translation="MFRVRIIQALLCAVATLQVIAADPTFLCRHVADIQPTQADLTTA
                     TAHYKPIFSIGDPDQQQLKSIARYGELTVDPGGSTETVSYPAEEQIYFVLEGTGVLLY
                     DSQKYPIKRDDFMYLPVDIRHGAANASSTPLRLLVMGFKIPDGLQVPPTQKLMLANVD
                     DVELRQLAGHGSTSQFKLLMGSTTARPDRHKLATTNTVIEMFIIDFSPGGTNIPHHHD
                     TAEEIYYVLRGHGDMVAGGGADGNEGRYASREGDAYFFRLNATVGFHSGSKAGEEHDL
                     VLAVRSKYPFSRERNR"
     gene            complement(6661..8070)
                     /locus_tag="VLE22_02085"
     CDS             complement(6661..8070)
                     /locus_tag="VLE22_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HSB13217.1"
                     /translation="MTENGVQAPVPQLRRELGIPQATSINMSNMIGIGPFITVPVIIA
                     TLGGPQSLLSWGVGALLALCDGLVVAELGAALPGSGGSYVFLRDSLGSARWGRLMAWM
                     FTWQFLFSGPLEIASAGIGMMQYLSYLFPPLAFHPLLMKLAAGMICALVVFTLYRKIH
                     DVARLMFILWITALITTGWVIASGLAAADPRLLFDIPAGSLRPNWAFLAGLGNGTVLV
                     IYNYLGYNQVCYLGGEVKRPERTIPVAVILSILGVTLIDVLVSASFVSVVPWREMLKP
                     GTPAHNAVASVFMSRIYGGWAAVTITVLVLATSFACVFALMLGYSRIPFAAAQDGVFF
                     RWFGALHPRGAFPHRSLLLVGALCIVACFFELEQIITALILARILVQFVGQIVALFVL
                     RRFRPEIALPFRMWVYPLPAVVALAGWLYVFCSPIVQPGGWKYALYAFGTIATGLAAF
                     LMNARRKHNWPFQGHAATS"
     gene            8164..9246
                     /locus_tag="VLE22_02090"
     CDS             8164..9246
                     /locus_tag="VLE22_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSB13218.1"
                     /translation="MKRIGIPEIAKLANVTIGTVDRALHGRPGISESTRQRVLEIARN
                     VGYQPNLAARALSVRRRPIRIGVSIPREIHYHFDQLQQGIKAEAQRAELLGVETIWAQ
                     TDRLGAAEPEKVAELLRNEPRALILTPGNPQQLIPVINEAERRNIRVICVDTDAPASI
                     RSSAVCVNATVSGRVAAELLGAMIPAGSSVAVVTGMLHIEDHSKKAEGFQEAFPQFCQ
                     GGRVAEIVEGHEDEDEAFQKCFALLERNESLAGMYVNTANCLPVCRAIGALGLAGRFK
                     LITTDLFDEMRPYFEKGTISASISSRPYVQGELAMRLVVDHLVNGAVLPKAHYLAPQV
                     VMRSTFHLFREMRQAVLAPAPASVAR"
     gene            complement(9255..10325)
                     /locus_tag="VLE22_02095"
     CDS             complement(9255..10325)
                     /locus_tag="VLE22_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB13219.1"
                     /translation="MRFLRLLPVFAVTLPLPAAGQETPPEKILLNDFRPVSIFKIPIT
                     EVSKAKYPVIDLHTHPYAETRAQLATWVELMDLSGIEKTIVLTGAVGAKFDALYQLYS
                     EYPRRFQLWCGFDYTALEESGFGPAAVKELERCYRAGARGVGEISDKGRGLNRRTQPG
                     GVHPDDVRLDPLWEKCAELGMPVNIHIADGIWDYEKMDIHNDGLMDAVSWRLDNQPGI
                     VGHAGMIDILERTLKKHPRTIFVACHFVNLIHDLKLLGQLLDRYPNLYTDISGRFAEM
                     GTIPRSTAQFCEKYAGRIVYGTDLGTDKRMYGITFRLLETLDEHYYERGEFNNHWTMN
                     ALGLSDSALKHLYRDTALKIIK"
     gene            complement(10908..11663)
                     /locus_tag="VLE22_02100"
     CDS             complement(10908..11663)
                     /locus_tag="VLE22_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB13220.1"
                     /translation="MHTGNWRYHWSTDDLAPIVRREGIRFALVSALESPPKRQVDSNR
                     EVLKEVAKYPDLFGALAWANPLEEGWREDLTATLDTGRCYGIKIHPARYKYDLSKFPE
                     ILRGVFEVARQYELPILTHTDEKFSPPKNFLALIEEFPEVPVVFYHSYPMIDSIAIAL
                     THANVYVENSWVADNPDFVLVAVKALGSQRVLYGTDAPVSFGWKPGEPLPESQRFFAR
                     RIKYIMGLPLPAQAKEDILFRNARRLFKLRLPK"
     gene            complement(12102..12228)
                     /locus_tag="VLE22_02105"
                     /pseudo
     CDS             complement(12102..12228)
                     /locus_tag="VLE22_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            12539..13687
                     /locus_tag="VLE22_02110"
     CDS             12539..13687
                     /locus_tag="VLE22_02110"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13221.1"
                     /translation="MDPISRSDLRQLIDIEQEPCVSIYFPTHRAGVHTLQDPIRLKNL
                     IREAERRLEERGDQAPVIREAIEPLRELVDQHEFWQHQADGLAVFRSRDIFRRYRVSF
                     SVPELAVVARRFHLKPLLPAVEANQRFRVLALSQDAAKVYEANHQSITEIEIQDMPES
                     PISPGVRSAERQLQSHTAGHQSSKRRFAIFHGHGADEEDRKQLLLSYFRRVDDTVRKT
                     CSDEHTPVVLAAVDYLCPIYRQASSNPNLLTEEIHGNPSSFPSEELHRLAWGIASSHF
                     QEDRTKAADEYHQLWHTQRASKDLSVIVTAARQGRVKVLFVAVGVQEWGHVDPSSAEV
                     VQVGQRSSQDQDLLNVAAMETFIAGGTVYAVPPDEVPGRGSVAAVFRY"
     gene            13706..14806
                     /locus_tag="VLE22_02115"
     CDS             13706..14806
                     /locus_tag="VLE22_02115"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSB13222.1"
                     /translation="MKPIWDSLLRHWTELVVPFVILLAVMATGWFARRFLFHVLERWA
                     ANSKARFDDLLVRTLRGPFMLWVMILGLHLAVQSSELPTRVTALVAKTLLILWIVSLT
                     VVAAKLAGSLVKLYGSGMASALPVTTLTQSLASLAVATVGILILLDTLHISVTPILTA
                     LGVGGLAVALALQDTLSNLFAGFYVSLAGQVRVGDYVKLDSTEEGYVTDISWRSTTLR
                     ALPNNLIVVPNAKLAQAIVTNYHLPEKRMSLLLPVSVSYDSDPETVERILLEEAVSGA
                     REIPGLLAEPAPFVRFIPGFGDSSLDFTLICQVAEFVDQYLAQHELRKRIFKRFRREG
                     IEIPFPIRTLYIKDAASSGAANHVPHHGGLGR"
     gene            14803..15450
                     /locus_tag="VLE22_02120"
     CDS             14803..15450
                     /locus_tag="VLE22_02120"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HSB13223.1"
                     /translation="MNHTEPFVFYTERRLVALTGQQASNLGQLLGNLHDVPGSSIFYH
                     THHMYLSHHFETPAFSNDFALWVSEALQEGALGEKLAAIDLLSFTSIRELREAIVATI
                     EQQIGEDGRRLRECPPGDELHFCRSKSFIMPTGLIARDPKEFFEVLPKVTNVSLFFHF
                     FEARLRLGRPTNDFSQWLAWRGEEILAKSIDALDPYSMTLDEFKERIIALSGNRG"
     gene            15454..16683
                     /locus_tag="VLE22_02125"
     CDS             15454..16683
                     /locus_tag="VLE22_02125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB13224.1"
                     /translation="MAIRLADYEEIVGREVIQEMHVLAERVKSRRLQNINSTPVGGGV
                     AEILTRMIPLLRELGIEATWDVIKGDQAFFNVTKAFHNALHGKKEDVTDEMLEVFRAT
                     TEANLREMNISGDIVLVHDPQPAGLVDRKREIGRQWIWRCHIDVSAPDARVWEFLRPY
                     VEQYDAAIFSMPDFAQRLPISQYMVAPSIDPLADKNVELTPAFITQVLEKYHLNPERP
                     IITQISRFDRLKDPLGVIAAYKMVKRRHDCQLVLAGGGASDDPEGEEVLREVEQQAAD
                     DRDIHILLLPPFSDLEINALVRGSTVVFQKSIKEGFGLTVSEALWKRKPVIGGAVGGI
                     KLQVINGVTGFLVHSPEGAAKRLVQLLGDRTLRARLGDNGYQHVKQNFLLTRHVKDYM
                     LVMLALDHPVEDIVHLG"
     gene            16702..17586
                     /locus_tag="VLE22_02130"
     CDS             16702..17586
                     /locus_tag="VLE22_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSB13225.1"
                     /translation="MGKLSRRWRLYGNALLHRKFANWLWRRIWADDIPGRAAQLSYYF
                     LLSLFPLLICLSALLGYFFAAQTRLEQRLLDYLGSIMPSAAFDVVKAATHELLQTRGG
                     GKLSFGLLVALWLASSGMEAVTEGLNVAFQVEEARPWWRRRLVALGLTVNGALLAGGA
                     LGLALAGGWGGKILTDIVGLGDIWSPVWRATQWIASILFLLFAISLVYFFGPNLRGRR
                     RTQVMLPGAIVALICWLTASAGLRLYLDNFSSFGRTYGSLGAVIALLLWLYLTGAALL
                     LGAEINSGIQARINETPH"
BASE COUNT         3548 a         5366 c         5265 g         3552 t
ORIGIN      
        1 atgctcaata ttgtcggccg gggcatggcg gggcgcacga cggtcgagca ggataccacc
       61 gatatggatc ccgagcggac cgcctccgaa gctgcgcgct atccggggct cgtcgtcggc
      121 atcaagatcg cccactatgc cggtccggaa tggatcgctg tcgaacgggc ggttgaggct
      181 ggcaggcggg cggatctacc cgtgatggtt gacttcgcgg aattccgccc cgagcggccg
      241 ttccaggacc tggtcgtgaa gaagctacgg cccggagaca tttacacgca cctgtatttg
      301 ggtgcggtgc ccatgctcga cccgcaaggg cgtgtgttgc cttacctgtt tgaggcaagg
      361 aaacgcggag tgatcttcga tgtcgggcat ggcgcaggaa gctttttgtt ccggcaggcg
      421 gttcccgccg tccggcaggg ttttgttccc gattcgatct cgaccgacct ccatgtgggc
      481 agcatgaacg caggcatgaa agacatgctc aacgtcatgt cgaagttcct gaatatgggc
      541 atgtcgctgg aggacgtgat tctgctctcc acttggaacc cggcgcggga gatccgccac
      601 acggaactcg ggcacctctc ggtcgggtcc tcggccgaca tcgccgttct tcgagtggtg
      661 gatggcaatt ttggcttcgt cgattccagc cgtagacgcc tccagggcac gaagaagctg
      721 gttgcggagc tcacgctgcg cgacggcaga gtggtctggg atttgaacgg cctcaccagc
      781 gacgactgga ccaagtagct ggcggctcta cttcaaccca gcttcgaact cgattcgctg
      841 ccccgccacc aggcggtcgc actcgatcgt gaggctggga ttcttgccgc ccgaggcgac
      901 cgtgaactga cgggtctcgc cgggctgcag gtacttgagc gactccttgg cgcgcacccc
      961 gttcaccgcc acgaaggcgt cgtagtaaat cggcgccacg cccgtgtttg cgaccgcgac
     1021 gcgcgaagcc ttcgccccgg cctcgaatga ggccaccttg aacttgtagc cgcacgccag
     1081 gccgtgttcc cggatgcgct ccatagtggt ccagcgcggc cactgctcgc agatcataaa
     1141 ggtgatgccg aagttcctgg tctccctgct ccatgcctcg gccacgtgct ctgctcgctc
     1201 ctttcccggg aaaagtatct cgccacccat gggggagtgc ttgtagcggt cgcgcccgaa
     1261 gaattcccac ccttccaggt tgtagctggg cgaccaggcc agatgaaagg tgtcgtcgaa
     1321 gatcccgaac ctgagtttca acaactgtgg ctgagaggcg aacggcgtgc gcttcgccac
     1381 gtgggcgtcc tgcgagatca tccagggcgt gtgctggaga accttcgtca ggtggtgaaa
     1441 aaagcgcgct tggaactcca tgctcgggaa cgtctcgtca aggacttccg gaccggagta
     1501 gacgtgatac tccgaccaca agccgaagcc gacttccagg aatgcgagtc gcggatcgcg
     1561 gtcgtatttc tccgcgaact tttcgtagaa ttcgagaaac aagcgctgcc actcgaggtg
     1621 cgaccagtcc ggaaatcccg tgtcgcgccg ttcgctcttg gctaccctgt cggtgtagtc
     1681 cggcagcgcc ttgatgtact gcggcacgct gctcgggcgg ccggggtaag tgtcccagaa
     1741 acgcaagatc gcctggtgtt tgcggcgcgc gctggcatcg agtttccgct cgacgggcgt
     1801 ccagtcgtac tcgccacgct gcttcaccac gtcgctgtaa cgcatgtagg agtactcgag
     1861 ctggatcgcg tcggtttcgc tgtttttcga atcctcccac atgacaatgc ccgtcatggg
     1921 ctggacattc gtgatgcgac tctcgagcgg caccggccga aaggcatctc gcgccggcgt
     1981 cgggccgact gcggccacgg caaaggccag cgcgaccaac gcgtacctgc gtttcatgtc
     2041 agaccccctt ccgccagccg cgatcatcga attgcttgag ttgaacgaca tacgagccgt
     2101 ccggatgtaa tggttgcgcg caggtccatc gcaccggatg gcggacacct tttgtctcga
     2161 gctcggcgcg aagctttact tgctggcctt ccaggcctgt gggcagaata aatgatgctt
     2221 gacgcaatcg tcccccgtgc gggtggccgc catcgagacc gccgcccacc cgcactctgc
     2281 cgtccatgct ttcgacatgc accctgagta cgccgggaac gccggcgacg ccatcgttag
     2341 cgatgccgag gatgatctcg ctcgtgccgt aacgctttcg ttgccagatg aaggagggac
     2401 gcacgcggta tcccaagcgg cggtgcagcg tttcgaacgc aaaaggatac ttctcataga
     2461 accgctgcaa attttcggcc tccgtccaca gcgcccagta attgccacac agatcgagga
     2521 cgtgcagggc ggcgttttcg cggtcgttga tgcccgctgc atcgaatacg agagcggctg
     2581 gatcgtagtg ccggtttcga ccctcctcca tcaccacagc cagccattgc gggcggttcg
     2641 acaggcactc cacctgaatc ggctcctcga tgtaaacggt actgtccgaa cgcagccagc
     2701 atccggcgcg cacggcaatg tcgaggcatt cccgatttcc cgttctgctg atatcaggct
     2761 gcgtgttgat ggccagcgga gtcttcttcc aagtgtcgat ctgaagccgg gtcatcgcgg
     2821 ccatggtctt ttccgccgtc aagtagtccg gaaacggatt ggggagcctg ttggtgtgtc
     2881 cttcgcccca gaatccgtac atcatcatgt ccatgaactc cagcagcgga tggccatcga
     2941 actcggccgc cagcaactcg ttcagttcct ggaacgcttt ctggaattcg gagtggtcgt
     3001 agcgtggctc tacgtaatcc cagtcctgcg gtttgccgcc gccctccccg cgcgcccggc
     3061 cgatcttcac cattggaatt ttgtgttgca gaaaatccgg tagcgcgagc tgctttggtt
     3121 gaaagttcgg gctagaaagc tggacccgga agccgacacg tagattgtgt cgcttggctg
     3181 catccagagt gagtttccag acagggttca agtccagctt gccaggctgc ttctgcacat
     3241 cgcgccaatc gcagcggatg tagagcacat cgacaaacgg aagactagcc agcttttcca
     3301 cggcctcttc cagcggctcc tggccgcggc gtgcggccag tgaagggcca ccctcctccc
     3361 aggtataagc caccagcccg gtcccgaagt tcttcacgtg cccacgggac ggattggtga
     3421 agccgatcgt ctgccagccc tcgtcctgat ctatgccgaa cggaccttgg ttaaggcggt
     3481 cggtcgcggc gggcgctgga ccgaaatcgt atccgtccca ccgatggtcg gggacatcgg
     3541 cgcgcagagc gggcgtgatt cccggcgcca gcgtggttat tgctgctgct cttaaaaagt
     3601 cacgtcgaat catatcgcgt ccccctcttc ggagagcgtg gctatggtaa cgtacacgta
     3661 gatggattgc aagacatagt ccagattatt aggcaacgat aacgaccttg acttcgcggg
     3721 aagcctgcac ctataatcca acggtccgaa gcggctcttg aacaggaccg agacgcctgc
     3781 gtaggccgca agttcgtgaa ggatgagatg aagactctgt ctggcgttct ttgcctgctg
     3841 gtaatcggat ttcccgcccg ggttggggca gcgccagacg accgcatggc ctccgggagt
     3901 aggggtttgg tcgtgtcggg cagaagcgct gcgactgctg ccggaatccg gattctcgag
     3961 cgggatggca acgctgccga cgccgccgca gcgacgctgc tcacgttgag cgtaaccacg
     4021 gtcggcgcat tctgtatcgg cggcgaagtt ccgctgctgg tctacgatgc gaagaagaac
     4081 gatgtgaagg ttctgtcagg gcagggcggc gcgcctcttg acccggcggc gatcgagtgg
     4141 tacctgaaga acggaatccc cagcaacgac gtgcgcgccg cggccgtgcc ggctgtcgtc
     4201 gacctggtgg tgaccctgtt gaagctgtat ggcacaaaga gctttggcga ggtcgtcggg
     4261 cccacgttgt ccatcctgga cgccggcggc cccacctggt acatcgatac ctcggaccgg
     4321 cgcaagatcg agacgggacg caactggcgt gctgagctgg cagtcacctt ccggaagctt
     4381 gtcgaagccg agcagaaagc caaaggctgg agagagagac ggctacaggc ggtcgcggat
     4441 cgtttctacc gtggtgatat cgcggatgcc ctcgagaagt ggtacatcgc gcagggcggg
     4501 tttctccgta aggcggatct ggccgcgcac agaacgctgg tggaagaacc tgtgcaggtc
     4561 agctatcgag gttacgcagt cctcaagtgc ggtccctgga cccaaggacc ctatctcctg
     4621 caatcattgc gcctgctgga aggcttcgac ttgaagggca tgggacatgc gtcagcggac
     4681 tacatccatg tggtgaccga agcaatgaag ctcgctctgg ccgaccggga cgaatactac
     4741 ggcgacccgc ggttcacgat ggttcccctg acgtctctcc tgtccgaccc ttatactcag
     4801 atgcgccggc cgctgatcga cataaagaaa gcctccctgg aagtacgccc aggcgatccg
     4861 taccgcatgc gtcctgttaa ggaaccgccg ccgccagcgg cagcatctca gggcggcacc
     4921 actgtcatgt gcgttgccga tcgttggggt aatgtcattg cggccacgcc gagcggattg
     4981 ggcagctcgg cgggcgtggc cggagacacc ggcatcatcc acggcgcccg cttggtcagc
     5041 ctgaatacct ggaagggcca tcccaattgc attgcacccg gaaaacggcc gcgcatcact
     5101 ctgacgccta cgcttgtgct caagaacggc aaaccgctgg tggccatcgc cgtggcggga
     5161 ggcgatctgc aggaccaggt cagcctccaa ctcatcctcg atcacatcga cttcggcatg
     5221 gtggcggagt cggcgtttca ggccccgcga ttcgccacgc gtcaccacac cggctcgttc
     5281 ggacaggaca ggccgcacct aggcagtctc caggtcagcg cgaaggtcag cgaatccgtg
     5341 cgcgaagacc tcaagcgccg gggccacctg gtgaccacca gctcgggcga aatcggagga
     5401 gccgcgatgc tgtccgtcga tcccgcgtcc cacgaggttc gcggagccgg gccgtcggcc
     5461 ggccgggtcg agtaacctac ttaccgaaga ttctcgacgc accggtgcac gcattgatca
     5521 ccgtgaaggc gccatcctag tgaaccgggt cgccggcggt tggtctgccc gttacgagcc
     5581 cgaggcagaa gaataccgta accgccaatc gcgacatcac gatgccttaa cggtttcgct
     5641 cccgcgagaa cggatacttc gagcggaccg ccaacacaag gtcatgctcc tcgccggcct
     5701 tggacccact gtgaaaaccg acggtcgcat tgagccggaa gaaatacgcg tcgccctccc
     5761 tgctcgcgta cctcccttcg ttgccgtcag ctccacctcc cgccaccatg tcaccgtggc
     5821 cgcgcaacac gtagtagatt tcctccgcag tgtcgtgatg gtgagggatg ttcgtgcctc
     5881 ccggtgaaaa atcgatgatg aacatctcta ttacagtgtt ggttgtcgcc agcttgtgtc
     5941 tgtcgggcct ggctgtggtt gagcccatca gcaacttgaa ctgtgaggtg gagccgtgtc
     6001 ccgccagctg tctcagctcc acgtcgtcga cattcgccag catgagcttc tgtgtcggcg
     6061 gtacctgcaa tccgtcggga atcttgaatc ccatcaccag cagccgtaac ggggtgctgg
     6121 atgcgttcgc cgcaccatgc ctgatatcca ccggcagata catgaagtcg tcccgcttta
     6181 tcgggtattt ctggctatcg tagagcagca cgccggtgcc ttcgaggacg aagtagatct
     6241 gttcctcggc cgggtagctc acggtctcgg tactgccgcc gggatccact gtcagttcgc
     6301 cgtaccgggc gatgctcttg agctgttgct ggtcgggatc tccgatggag aaaatcggtt
     6361 tgtagtgcgc ggtcgcggtc gtcaggtccg cctgcgttgg ctggatatcc gcaacgtgcc
     6421 tgcacaggaa agtaggatcc gccgcaatca cctgcaaggt cgcgacggca caaagaagcg
     6481 cctgtatgat tcttacgcga aacacaacgt tagtccacct cctcctctta cttacttcga
     6541 cggacgccac gcgatcagct gccgccgctg cgctggccat ggcggttagc gccacgccgg
     6601 atctgatgcc tacagccgat ctcaagaatg cccgtctcag gctcattttg cacctcctgt
     6661 tcacgaagta gcagcgtgcc cctggaaggg ccagttgtgc ttccgccgag cgttcatcag
     6721 aaacgccgcc aatccggtgg caatggttcc gaaggcgtag agtgcgtact tccatccgcc
     6781 tggctgcacg atcgggctgc agaaaacgta caaccatccc gccagggcca cgaccgccgg
     6841 cagggggtaa acccacatgc gaaacggcag cgcgatttcg gggcggaacc gtcgcaggac
     6901 gaagagagct acgatctgtc ccacgaactg aaccaggatc cgcgccagga tcagcgctgt
     6961 aatgatctgt tcgagttcga aaaaacaggc cacgatgcac agcgcaccca ccagcagaag
     7021 cgagcggtgc gggaaagccc cccgcggatg cagagccccg aaccagcgga agaaaacccc
     7081 gtcctgggca gccgcgaatg ggatgcggga gtagccgagc atcagcgcga acacgcacgc
     7141 gaacgaggtt gccaggacca gcaccgttat ggtaacagcg gcccatccgc catagatccg
     7201 gctcatgaaa accgaggcga cagcgttgtg ggccggcgtg cccggcttca gcatctccct
     7261 ccagggcact accgacacga aactcgctga aactaggacg tcgatcagcg tgacacccag
     7321 aatcgacaga atgacggcaa ccggtatggt tcgctcgggt cgcttcactt ccccacccaa
     7381 gtagcagacc tggttgtatc ccagatagtt gtagatcacc agcaccgtcc cattgccgag
     7441 ccccgccaga aaagcccagt tgggccgcag gctgccggcc ggaatatcga acagcagccg
     7501 tggatccgcg gcggccaaac cgctcgcgat cacccagccg gtggtgatta gtgcggtgat
     7561 ccacagaatg aacatgagcc gtgccacgtc atggatcttc cgatatagcg tgaagacgac
     7621 cagcgcgcaa atcataccgg ccgccaactt catcagcagc ggatggaagg ccagcggagg
     7681 aaacaggtaa ctcaagtact gcatcatgcc gatgccggcc gaggcaatct ccaggggccc
     7741 actgaacagg aactgccaag tgaacatcca cgccatcagc cgcccccagc gcgcgctgcc
     7801 caggctgtcc cggaggaaga cataggaacc tccggaaccg ggcagcgcgg cgcccaactc
     7861 ggctaccact aggccgtcgc ataaagccag caacgcaccg acgccccaac tcagcagaga
     7921 ctgcgggccg cccagggtgg cgatgatgac aggcacggtg atgaacggcc caatcccgat
     7981 catgttggac atgttgatcg aggtcgcctg cgggattccg agttcccggc gcagttgcgg
     8041 aacgggcgcc tgaacgccgt tttctgtcat acgtgaacgt tacctcaagc tgtcaatagt
     8101 acaccatgat gcggtcggtg tggcggaatt ccaaggtata ataaaaattc ataaaggaga
     8161 agcatgaaac ggatcggcat cccggagatc gcgaagctgg cgaatgtcac catcggcacg
     8221 gtagaccgcg ccctgcatgg gcggccaggc atcagcgaga gcacgcggca acgcgttctg
     8281 gagatcgccc ggaatgtcgg ttaccagccc aatctcgcag cccgggcgtt atcggtaagg
     8341 cgcaggccca ttcgaatagg cgtatcgatt ccccgtgaga ttcactatca tttcgatcag
     8401 cttcagcagg ggatcaaagc cgaggcgcaa cgggccgagc tattgggcgt ggaaaccatt
     8461 tgggcgcaaa ccgaccgctt gggcgcggcg gaaccggaaa aagtggcgga attgctgcgc
     8521 aacgagcccc gggcgctgat tttgacgcct ggcaaccccc agcagctgat ccctgtgatc
     8581 aacgaagcgg aacgccgcaa catccgtgtc atttgcgtcg acaccgatgc gccggccagc
     8641 atccgctcgt cggcagtctg cgtgaacgcc accgtgagcg gacgtgtcgc cgccgaactg
     8701 ctcggcgcca tgattcccgc cggatcctca gtggcggtcg tcaccggcat gttgcacatt
     8761 gaggatcata gtaagaaggc cgagggcttt caggaagcct ttccgcagtt ctgccaaggt
     8821 ggtcgcgtcg cagaaattgt cgaaggccac gaagacgaag acgaagcctt tcagaagtgc
     8881 ttcgcactcc tggagcggaa cgaatcgctc gcgggcatgt acgtcaacac ggccaattgc
     8941 ctccccgtgt gccgggccat tggagcgctg ggactcgccg gccgcttcaa gctcattacc
     9001 actgacctgt tcgacgagat gcggccatac ttcgagaaag gaacgatctc agcctccatc
     9061 tccagccgcc cgtacgtcca gggtgaactc gctatgcgcc tagtggtgga ccatcttgtc
     9121 aatggagccg tcctgcccaa ggctcactat cttgctccgc aagtggtgat gcgctccacc
     9181 ttccacctgt tccgcgagat gcgccaggct gtgctggcgc ctgcgcctgc ttcggtcgcc
     9241 aggtgaaact ccccttactt tatgattttc aaggccgtgt cgcgatacag gtgcttcagc
     9301 gcggaatccg acaagccgag ggcgttcatt gtccagtggt tgttgaactc gcccctttcg
     9361 tagtagtgct cgtcgagtgt ttccagtagt cggaaggtga tgccgtacat acgcttgtcg
     9421 gtccccaagt ccgtcccgta cacgatccgc cccgcgtact tctcacagaa ttgcgccgtg
     9481 gaacgcggaa tggtccccat ctccgcgaac cgtccggaga tgtccgtata caggttcgga
     9541 tagcggtcca gcaattgccc caggagcttc aagtcgtgaa tcaggttcac gaagtggcac
     9601 gcaacgaata tcgttctcgg gtgcttcttg agcgtccgtt ccaggatgtc tatcatcccg
     9661 gcatggccga cgatacccgg ttggttgtcc agccgccaac tcactgcatc catcaggccg
     9721 tcgttatgga tgtccatctt ctcgtaatcc cagatgccgt cggcgatgtg aatattcacc
     9781 ggcataccca attcggcgca cttctcccac agcgggtcga gccggacatc gtcgggatgg
     9841 acgccgccgg gctgcgttct cctgttcagg cctctgccct tatcgctgat ctcgccaact
     9901 ccgcgcgcgc ctgcccggta gcatctttcg agttccttca ccgcggcggg cccaaatccc
     9961 gattcttcga gagcagtgta atcgaagccg caccacaact ggaaccggcg cgggtattca
    10021 gagtacaact ggtagagcgc gtcgaacttc gcgcccacgg ccccagtcaa aacgatggtt
    10081 ttttcaatgc ctgacaggtc cattagttcg acccacgtcg caagttgcgc gcgcgtctcg
    10141 gcgtacggat gcgtgtgcag atcgatcacc ggatacttgg ccttggacac ttccgtaatc
    10201 ggaatcttga agatcgacac cggccggaag tcattcagaa ggatcttttc cggtggcgtc
    10261 tcttgcccgg ccgccggcag aggaagcgtc accgcgaaga ccggcagtag tctcaggaac
    10321 cgcatagacc gtctatacac ccttagtgcc tgtcgaagca agcccacgtg tccgttaccg
    10381 ccaggaatca gcgctgccac gcctggggtc gcgttggagc tacggttgtg ctaaactttc
    10441 acgttaacct taacggatgc gagacgaatt caactgaaac ccgtccaccg cggggtctgt
    10501 ccagcgcgtt tgcccccatc aaagaacgtt cacaagacca ttccgagagc ccgatcaaca
    10561 ttccgaaacg ccggtcgttt tcgatcggca tgacgtcgga acgtcgatcg gcatcacctc
    10621 ggaatcccga tcgccatcag ttcggaatca tgatcggctt gcccaggaat acgcagttgt
    10681 ccgaagaacg cgatcgagtg gaagatgtcc tgctgctcgc gaaacatcat cggacagtgg
    10741 cgtaggatcg gatgacgaac cacagcttcc tttcggagga ttaccaggtt caggagataa
    10801 cattctcttc aggcgtgaaa gtctgggtcg attccaagca taagacattc aagatcagcg
    10861 gcctgcctgg aattgaggcg gtgaaacggc agacacaatg acacagtcta cttcggcaac
    10921 ctgagcttga acaggcgccg ggcattgcgg aaaaggatgt cctctttggc ctgtgcgggg
    10981 agaggcaggc ccatgatgta cttgatccgc cgtgcgaaga accgttggga ctcggggaga
    11041 ggttccccag gcttccaacc gaatgacact ggtgcgtccg tgccgtagag gacacgctgg
    11101 gatcccagcg ccttcaccgc cacgaggacg aagtcgggat tgtccgccac ccatgaattc
    11161 tcgacataca cgttggcgtg ggttaaagca atggcgatcg agtcgatcat cgggtacgag
    11221 tggtagaaca ctaccggaac ttccgggaac tcctcgatca acgcaagaaa attcttgggc
    11281 ggactgaact tctcgtccgt gtgcgtcagg atcggcagct cgtactgacg agcgacctcg
    11341 aacactcctc tcaggatttc ggggaacttg ctcagatcgt acttgtagcg ggcggggtgg
    11401 atcttgatgc cgtagcaacg gcccgtgtcg agtgttgcag tcagatcttc ccgccagcct
    11461 tcctccagcg gattggccca tgctaatgcc ccaaagagat ccgggtattt ggccacttct
    11521 ttgagcactt ctctgtttga gtccacctgt cgtttcggtg gcgactccaa tgcggacacg
    11581 agagcaaagc ggatcccctc ccgcctcaca atcggcgcca gatcgtccgt ggaccagtga
    11641 tatcgccagt tgccagtgtg catgtggcaa tcgaccactg gaaccgaggg ctcaccgcct
    11701 aacagcggta agcgggaaca gcaggcaagt agcacaactg tcaacgccaa gatacatcgc
    11761 atcttccgcc agaacaccgc tgagcatacc acgtcaccac ggtaccgtca gagcggagca
    11821 ctcttgaagg actgcgtatc cgattggcgg atcacaaccc cgcttgtggc tctgccgaaa
    11881 aacaggttgc gatgtacttc agggcactat catgtcaacg ccgcttgaaa tctccccatt
    11941 tcgccggttg aaaattcccc acccataggc acctcaaatc tgcttttccg cagccccgcg
    12001 ccaagcggag ccccgcgccg cttttgggca cgttttgggc ggagcgaaag ccgggctgtg
    12061 gaaaagctcg ccgccacccc gccctcctgt tcccgcgagc ctaacagccc cgcccgccgc
    12121 cgatccttca gccgatagct ctccccgcga atgtttgatg gtcgtcgaat gatgcagcag
    12181 ccgacccaga atcgcggtcg caatgatcgg atcgccgaag atcgacccgg gccgaccagc
    12241 gtttccaggg tcgcggcagt ttgctgagtc ccggcaaact ggagatcatc tcaccaccat
    12301 tcttcaagcc aagtccgtcg cggcgccgcg tcccatgacg aagtcgcgtc cagggggcgc
    12361 ccagcaggaa cggagtggtt cccaaacatc aggatagtgg cttggacgag gcgtgaaaac
    12421 aacctaagcc cttttgggac gctggttcac cccttgtctg gatgttgatt ctcccggcga
    12481 aaacgtagtt tgggactagg gcattgcgtc cgagaacgcc cacgcccctg ggaggcttat
    12541 ggatcccatc agcaggagtg atcttcggca gttgatcgac atcgaacaag agccgtgcgt
    12601 ctccatctat ttcccaacgc atcgtgccgg tgtgcacaca ctccaggacc cgatccgact
    12661 caagaacctg atccgtgagg cggagcggcg gctggaggaa cggggtgacc aggcgccggt
    12721 catccgggaa gcgatcgagc ctctgcgaga acttgtcgac caacatgagt tctggcagca
    12781 ccaagccgat ggccttgcgg tgttccgctc gcgggacatc ttccgccgct atcgcgtttc
    12841 cttttcggtg ccagaactgg cagtggtggc caggcgcttt cacctaaaac ctctgcttcc
    12901 cgcagtggag gcgaaccagc gcttccgtgt tctcgcgctg agccaggatg cggctaaggt
    12961 ctacgaagcg aaccaccaga gcatcaccga gatcgagatc caggacatgc cggaaagtcc
    13021 catctcgcct ggggtacgca gcgcggagcg ccagttacag agccatacgg ccggccacca
    13081 gtcttccaag cggcgcttcg caatctttca cggccacggc gcggacgaag aggaccgaaa
    13141 gcagcttctc ctttcctatt tccgccgcgt ggacgacacc gtgcggaaaa cgtgtagcga
    13201 cgaacacact ccggttgtgc ttgcggcagt ggattacttg tgcccgatct atcggcaagc
    13261 aagctccaat ccaaacctgc tgaccgagga gatccatggg aacccctcca gtttcccatc
    13321 cgaggaactt catcggcttg cgtggggcat cgcgtcttcc catttccaag aggaccgcac
    13381 taaggcggcg gatgagtacc atcaactgtg gcacacgcag cgcgcgtcga aagatctctc
    13441 ggttatcgtg accgcggccc ggcaggggag agttaaggtt ctattcgtgg cagtaggagt
    13501 ccaggagtgg ggacatgtgg acccgtcgag cgccgaagtc gtccaggttg gacagcgctc
    13561 gtcgcaagat caggatctgt taaacgtcgc agccatggag acgttcatcg cgggaggcac
    13621 tgtttacgcc gtgcctcccg acgaagtacc gggacgagga tcggtcgctg cggtattccg
    13681 ctactgacag gagggcacaa gtgagatgaa gccaatctgg gacagcttat tgcggcattg
    13741 gactgaactg gtcgtgcctt ttgtcatcct cctcgctgtg atggccaccg gctggttcgc
    13801 ccgacgtttt ctctttcacg ttcttgaaag gtgggcggcg aacagcaagg cccgatttga
    13861 cgatttgttg gtccgaacgc tgcgcgggcc attcatgctt tgggtcatga ttctgggcct
    13921 tcacttggcg gttcagtcgt cagagctgcc gacgcgcgtc acggcactcg tcgccaagac
    13981 attgctgatt ctttggatcg tatccttgac ggtggtggca gcgaagctgg ccggctcatt
    14041 ggtcaagctc tacggcagcg gcatggcttc cgccctgcca gtcacgacgc ttacccaaag
    14101 cctggccagc ctagccgtgg ccacggtcgg gatattgatc ctgttagaca cgctccacat
    14161 atctgtgacg cccattctga ccgccctggg cgtcggcggc cttgcggtcg ctttggccct
    14221 ccaggacacg ctctcgaatc tcttcgctgg attctatgta agcctggcgg gacaagtgcg
    14281 tgtcggagac tacgtgaagc tggactctac ggaggagggc tacgtgacgg acatcagttg
    14341 gcgcagcacg accttgcggg cgctgccgaa caacctgatc gtagtgccca acgccaaact
    14401 cgcgcaagcg atcgtcacca actaccatct tccagaaaag agaatgtccc tgcttctgcc
    14461 ggtcagcgtc agctacgaca gcgatccgga aacagtcgaa cgaatcctgc tcgaggaggc
    14521 tgtctccggg gcgcgagaga ttccaggact gttggccgag ccggcaccgt tcgttcgttt
    14581 catccccggc tttggtgatt cttcccttga cttcactctg atttgccagg tggccgagtt
    14641 cgtggaccag tacctggcgc aacatgaatt gcgaaagcga atcttcaagc ggttccggcg
    14701 agaaggaatt gagattccat tcccgatccg gacgctgtac ataaaggatg ctgccagcag
    14761 cggtgcggct aaccatgtgc cgcatcatgg gggcctcgga cggtgaatca cacagagcca
    14821 tttgttttct acactgaacg caggttggtg gccttgaccg ggcagcaggc gagcaacctt
    14881 ggccaacttc tcgggaacct gcacgatgtt ccaggctctt cgatcttcta ccacactcat
    14941 cacatgtacc tttcgcacca tttcgagacg ccggcgttct cgaacgactt tgctctgtgg
    15001 gtgtcggagg cgttacagga aggtgcgtta ggcgagaagc tggcggcaat cgatcttctt
    15061 tcctttacct cgatccgtga actgcgggag gcgatcgtcg cgactatcga acagcaaatt
    15121 ggcgaggacg ggcggcgact gcgcgagtgc ccccctggcg acgaacttca tttctgccgg
    15181 tcgaagagct tcatcatgcc cacggggttg attgcccgtg atcccaagga gtttttcgag
    15241 gtactgccga aggtgacgaa cgtctcactt ttcttccact tttttgaagc gcggctgcga
    15301 ctcggcaggc cgacgaacga cttctcccag tggctggcct ggcgaggaga agagatcctg
    15361 gcgaagtcga tcgacgccct ggatccgtac agcatgacac tggacgaatt caaagaacgc
    15421 atcatcgcgc tcagcgggaa cagggggtag gtcttggcga ttcggctggc cgattacgag
    15481 gagatcgtgg gtcgcgaagt catccaggaa atgcacgtcc tagccgagcg cgtcaagagc
    15541 cggcgcttgc agaatatcaa ctcgacgccg gttggcggag gcgttgccga gatcctgacg
    15601 cgcatgattc ccctgttgcg cgaattgggg atcgaagcca cctgggatgt catcaagggc
    15661 gaccaagcct tcttcaacgt tacgaaggcc ttccacaacg cgcttcacgg aaagaaggaa
    15721 gatgtcacgg atgaaatgct agaggtcttc cgggcgacga cagaggccaa cctgcgggag
    15781 atgaacatct ccggtgacat cgtgctcgtg catgacccgc agcccgccgg cctcgttgat
    15841 cgaaagcgtg agattggacg ccagtggatc tggaggtgtc acatcgacgt ctccgccccc
    15901 gatgcgcgag tctgggagtt cttgaggccc tatgtggagc aatacgacgc cgccatcttc
    15961 tcgatgcccg acttcgcgca gcgacttccc atttctcagt acatggtggc gccgtcaatc
    16021 gaccctttgg ccgacaagaa cgtggaactc acccccgcct tcataaccca ggttctggag
    16081 aagtaccacc tcaatccgga acgtccgatc atcacgcaga tctcccggtt cgaccggcta
    16141 aaggatccct taggggtgat cgccgcctac aagatggtca aaaggcgcca tgattgccag
    16201 cttgttctcg ccggtggtgg cgccagcgac gatccggaag gtgaagaagt gctgcgtgaa
    16261 gtcgagcagc aggccgccga cgatcgggac attcacatcc tcctgttgcc gccgttcagc
    16321 gatctcgaaa tcaatgcctt ggtgcgcggg tcaactgtgg tctttcagaa gtcgatcaag
    16381 gaaggcttcg gactcaccgt aagcgaggct ctctggaagc gaaagccggt catcggcggc
    16441 gccgtgggag gtatcaagct tcaagtgatt aacggcgtaa ccgggttcct tgtccactcg
    16501 cccgagggtg cggccaaacg cctggtccag ctcctgggtg accgcacgct acgggcaagg
    16561 ctaggggaca acggctacca gcacgtgaag caaaacttcc ttttgacgcg gcatgtgaaa
    16621 gattacatgc tggtgatgct cgccctggat cacccggtcg aagatatcgt ccatctcgga
    16681 tgaggaatcg tgctagagcc aatgggcaag ctttcccggc gatggcgcct ctatggtaac
    16741 gcgctcctcc acagaaagtt tgccaactgg ctctggcgtc gaatctgggc ggacgacatt
    16801 ccgggtcgag cagcgcagct ctcctactac ttcctgctgt cgctgtttcc cctcttgatt
    16861 tgcctctcgg cacttctggg gtatttcttt gcggcccaga ccaggcttga gcagcgcctt
    16921 ctcgattatc tcggttccat aatgccgagc gccgcctttg atgtcgtcaa ggcagcgaca
    16981 cacgagctcc tgcaaacaag gggcggcggg aaactctcct tcggtctact cgtagcgctt
    17041 tggctggcct catcgggcat ggaagccgtc accgaaggct tgaacgtcgc gtttcaagtc
    17101 gaagaggctc gcccctggtg gagacggcgc ctggttgcgc tgggcctgac agttaacggc
    17161 gcgcttctgg cgggaggcgc gcttggactg gcgctggcgg gtggctgggg tgggaagatt
    17221 ctgacggata tcgtgggtct cggcgatatc tggagcccgg tttggcgcgc gacgcagtgg
    17281 attgcgagca ttttattcct cctgttcgcg atcagcctgg tctacttctt cgggcccaac
    17341 ctcaggggga ggcgcaggac gcaggtcatg ttgcctggcg ccatagtcgc acttatctgc
    17401 tggctcacag catccgccgg gctacgcctg tatctggata actttagttc ttttggccgg
    17461 acctacggct cccttggcgc cgtgatcgcc ctgctgctct ggttgtactt gactggcgcc
    17521 gcgctcctgc tgggggcgga gatcaactca gggattcaag cacgaattaa cgaaacaccg
    17581 cactgatgat ctctgcttcg aagaactgga agtgtcgttc ggtgtagggc caccacccga
    17641 ccgagtagcg cgccgggatc gctatcttgg aatgggaatg aggctacctt tcagcgcaga
    17701 agatcttctt gatgtctttc gaggctacaa c
//
LOCUS       DATIFQ010000045         2722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_970701, whole genome shotgun sequence.
ACCESSION   DATIFQ010000045 DATIFQ010000000
VERSION     DATIFQ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970701"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VLE22_02135"
     CDS             complement(<1..686)
                     /locus_tag="VLE22_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB13226.1"
                     /translation="MSTITTKDGTSIFYKDWGPRNAQPIVFHHGWPLSSDDWDTQMLY
                     FLGKGFRVVALDRRGHGRSSQVSDGHDMDHYAADAAAVVAHLDLRDAIHIGHSTGGGE
                     ATHYVARHGKGRVAKLVLIGAVPPIMVKTPGNPGGLPIEVFDGLRQQLAANRAQFYWE
                     FASGPFYSYNRPDAKVSQAVIENWWRQGMMGGAKAHYDGIKAFSETDFTEDLKIIDVP
                     TLVMHGDDDQ"
     gene            complement(1114..2058)
                     /locus_tag="VLE22_02140"
     CDS             complement(1114..2058)
                     /locus_tag="VLE22_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB13227.1"
                     /translation="MNERKISSIIEPAAPAAFGRRPGLDELVPSRYALRVGEIDVLVV
                     SDGVLSLPGAMLGHNADPAVRAAWLEDMFLPDVFEWALNEVVVRSGGRTILIDSGLGA
                     DPDLNLPRAGRLVQRLEAAGIDLASVTDVVLTHLHMDHIGGLLVDGVRDRLRPDLRVH
                     LAAAEAKFWESPDFSRVSMPPGFPDALRRAAKRFLNEYQSQMRPFDEEYEVAPGVLVR
                     RTGGHTPGHSVVRLASGGDRLTFAGDAVFAVGFDHPEWYNGFEHDPEEAARVRVRLLR
                     ELAATGEALVATHLSFPSVCHVAVAGDVFRWVPATWEY"
     gene            complement(2228..2383)
                     /locus_tag="VLE22_02145"
     CDS             complement(2228..2383)
                     /locus_tag="VLE22_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13228.1"
                     /translation="MLLRDTMSAKVEDLPADATLMQGAEKMMPLDIGAMPVRDAESEP
                     RSEHRTE"
     gene            complement(2437..>2722)
                     /locus_tag="VLE22_02150"
     CDS             complement(2437..>2722)
                     /locus_tag="VLE22_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB13229.1"
                     /translation="NWWRQGMMGGAKAHYDGIKAFSETDFTEDLKIIDVPTLVMHGDD
                     DQIVPIANSALLSVKLLKNGLLKVYERFPHGMCTTHTEVINADLLKFLRS"
BASE COUNT          477 a          922 c          812 g          511 t
ORIGIN      
        1 atctggtcgt cgtcgccatg catgacgagc gtcggcacgt cgatgatctt caggtcctcg
       61 gtaaagtcgg tctccgagaa ggccttgatg ccatcgtaat gcgctttcgc accgcccatc
      121 atgccctggc gccaccagtt ctcgatcacg gcctgggaca ccttcgcgtc cggccggttg
      181 tagctgtaga aggggccgct cgcgaattcc caatagaact gcgcgcggtt ggcggcgagc
      241 tgttgccgga ggccgtcaaa tacctcgata ggcaagcctc ccggattgcc tggtgtcttc
      301 accatgatcg gcggcacggc gccgatgagc accagcttgg cgacacggcc cttgccgtgg
      361 cgcgcgacat agtgcgtcgc ttccccgccg ccggtcgaat ggccaatgtg gatggcgtcg
      421 cgcagatcga gatgcgcgac gaccgcggcg gcgtccgcgg cgtagtggtc catgtcgtga
      481 ccatcgctga cctggctcga ccggccatgg ccccgccggt cgagggcgac gacgcgaaag
      541 cctttgccga ggaagtaaag catctgggtg tcccagtcat cggaactcag cggccagccg
      601 tgatgaaaca cgattggctg cgcgttcctg ggcccccaat ccttgtaaaa aatactcgtg
      661 ccgtccttgg tcgtgattgt tgacatgcca aggctccttt ctcgctggtt cgcgaggttg
      721 ctgtcagccg ctcggctgca ccttttcctg gtcgacgcaa cagccatcca ggcaccaacc
      781 tcgacaaccg caaattatct tcgcccttac taagcggaac aacgcgacac ctggcgcaag
      841 gtggttcggc cgaacccgag agtcgcctga ggcgaaaggc aagcggcacg tgatgcccga
      901 aagttcatgc gcccgtgctg ctcagcgcgg acctttgggc aacgataaca gcgcggcgcc
      961 gagcgcgtac aagaggtagc cattccatgc ggcgtttggc tcgcagttcc ctctccgccc
     1021 gaccaatcct cctcaggcgg gctgctgcgc tcttgccaga accagtatgc cagttgttct
     1081 gcaggcctag ttcggcccta acccaacaag cggtcaatat tcccaagtgg ccggtaccca
     1141 acgaaagaca tcgccggcga cagccacatg gcagacggac gggaacgaca ggtgagtggc
     1201 caccagcgcc tcgccggtcg ccgccagctc ccgcaaaaga cggacccgga cgcgggccgc
     1261 ctcctcgggg tcgtgttcga agccgttgta ccactcgggg tggtcgaacc cgaccgcgaa
     1321 cacggcgtcg ccggcgaacg tcagccggtc gccaccagac gccaggcgga ccacactgtg
     1381 cccgggggtg tggccgccgg tgcgacgcac gagcaccccc ggcgccacct cgtactcctc
     1441 atcgaacggc cgcatctggc tctggtactc gttcaagaac cgcttggccg ccctccgaag
     1501 cgcgtccggg aaccccggcg gcatggagac gcgggagaaa tcgggcgact cccagaactt
     1561 ggcctcggcg gccgccaggt ggacccgcag gtccggacgc agccggtccc tcaccccgtc
     1621 gacgagcagc ccgccaatgt ggtccatgtg caggtgggta agcaccacgt cggtcacgga
     1681 tgcgagatcg atgccggcgg cctccagtcg ctggaccaac cgcccggccc gcggcaagtt
     1741 caagtccggg tccgcgccga gcccagaatc gatgagtatg gtccggccgc cgctacgcac
     1801 cacgacctcg ttcagcgccc actcgaacac gtccggcagg aacatgtcct ccagccaggc
     1861 cgcccggacg gccgggtcgg cgttgtggcc caacatcgcg cctggcagcg atagcacccc
     1921 atcgctgacc accagcacgt caatctcgcc gactcgcagc gcgtagcgcg acggaaccaa
     1981 ctcgtcgagc cccggtctcc tgccgaacgc cgccggcgcg gccggctcga ttatcgaaga
     2041 aatcttccgt tcattcatgt tcggttctcc ttgttgttga gttccctcta tgaaaactgg
     2101 gcgtgattgc ccgacatttc ggctcacgtt cgcactggtc ctttaccgga accgcagcgc
     2161 gttgctcccc aacgcttccc tgccttcatc gagtctctcg ctcgatgccg agcgcctaca
     2221 agggtagtca ttccgtgcgg tgttcgcttc gcggttcgct ctcagcgtcc cggactggca
     2281 tggcgccgat gtccagtggc atcatctttt ccgcaccctg catcagcgtc gcatcggcgg
     2341 ggaggtcctc gactttcgca ctcatcgtgt ctcggagcag catggttggt ctcctttcgt
     2401 ttaagtgctt cttgtgcggt ccccgggtct gcccgctcaa gaccggagga atttcagcaa
     2461 atcggcgtta atgacctcgg tgtgcgtcgt gcacatgccg tgcggaaacc tctcgtaaac
     2521 ctttagcagg ccgttcttca gcagcttgac cgacaacaat gcggagttgg cgatcggcac
     2581 gatctggtcg tcgtcgccat gcatgacgag cgtcggcacg tcgatgatct tcaggtcctc
     2641 ggtaaagtcg gtctccgaga aggccttgat gccatcgtaa tgcgctttcg caccgcccat
     2701 catgccctgg cgccaccagt tc
//
LOCUS       DATIFQ010000046         3706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_779665, whole genome shotgun sequence.
ACCESSION   DATIFQ010000046 DATIFQ010000000
VERSION     DATIFQ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779665"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VLE22_02155"
     CDS             <1..1354
                     /locus_tag="VLE22_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13230.1"
                     /translation="IATWGIAAILMSNPLAGVGPSPPWAERAHAAIDQGRKIGAKTQR
                     ERDYIEAVAAYYEDWANRPERTRQLNRARAFEALAARYPNDDEAQIFSALYLAATQSL
                     ADQTYVTYLRAAEILEQQFAKHPDHPGVAHYLIHSYDAPPIAPKGLPAARRYARIAPA
                     APHALHMPSHIFTRVGAWSESVAANERSAVAAKQDKDFDEQLHAMDYLVYAYLQLARD
                     DDARRVMDEGATVTGTLRFAGPYAQAAMPARYMIERGDWKQAMKLEPRPSQFPFTEAL
                     THFARALGAARNGDTAAAEKDVKELARLRDTLKTAKNEYWATEVEASRLGAAAWTALA
                     QGKPDEALGLMQRAADIEDKNEKHIVTPGRIMPARELLGEMLLELKRPVEALKEFEAS
                     HVREPERLRGYYGAAQAAAQSGDQAKAKRYFARLVEVAGQGTARPELAQARAFLAANP
                     "
     gene            2368..2757
                     /locus_tag="VLE22_02160"
     CDS             2368..2757
                     /locus_tag="VLE22_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB13231.1"
                     /translation="MAKKTLLLVLLCLVSSPLMAQEPSVTPLMSKDLTDRPGKEVTMI
                     IVEYPPGGSDPVHRHNAHAFLYVLEGSVVMQVKGGKEITLTPGESFYEGPDDVHIVGR
                     NASGTKPAKFLVFLIKDKGAPVLVPVE"
     gene            2871..3620
                     /locus_tag="VLE22_02165"
     CDS             2871..3620
                     /locus_tag="VLE22_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB13232.1"
                     /translation="MKIVVIGGSGLIGTKLVNNLREHGHEAVAASPASGVDTLTGAGL
                     AEALTGTQVVVDVSNSPSFEDAAVLKFFETSTRNLLSAEAAAGVRHHVALSVVGADRI
                     PDSGYMRAKIAQEKLIKAATVPYTILRATQFFEFIGRIADGSTDGNTVRLTPALMQPV
                     AADDVAAELDRVALGEPVDGIVELAGPEPIRLDELARRLLSAKHDARQVTADVHARYF
                     GAEVNDQSLTPGDNPRVGPTRFDEWLDRLAA"
BASE COUNT          793 a         1125 c         1143 g          645 t
ORIGIN      
        1 gatcgccacg tggggcatcg ccgctatcct aatgtcgaac ccgctcgccg gcgtcggccc
       61 gtcgccgccg tgggctgagc gcgcgcacgc ggcgatcgac caggggcgga agatcggagc
      121 gaagacgcag cgcgagcgcg actacatcga ggccgtagcc gcttactacg aagactgggc
      181 gaaccggccc gagcgtacgc gccagctcaa tcgagcccga gccttcgagg cgctggccgc
      241 gcgctatccg aacgacgacg aggcgcagat cttctccgcc ctctacctcg ccgccacgca
      301 gtcgctggcc gatcaaacct acgtgaccta tctcagagcg gcggagatcc tggagcagca
      361 gttcgctaag catcctgacc accccggggt cgcccactat ctgatccaca gctacgacgc
      421 gccgccaatc gcgccgaagg ggctgccggc cgcccgccgc tacgcgcgca ttgctcccgc
      481 ggcgccgcac gcgctgcaca tgccgtcgca catcttcaca cgcgtcggcg cctggagtga
      541 atcggtcgcc gccaatgagc gctccgccgt ggccgcgaag caggataagg acttcgacga
      601 gcagctccac gcgatggact acctggtcta tgcgtacctg cagctcgcac gggatgatga
      661 cgcgcgccgc gtgatggacg aaggcgcaac ggtcaccggc acgctgcgct tcgccggtcc
      721 ctacgcgcag gcggcgatgc cggcgcgcta catgatcgag cgtggcgact ggaagcaggc
      781 aatgaagctg gagccgcgcc cgagtcagtt cccgttcacc gaggcgctca cgcacttcgc
      841 acgtgccctc ggcgctgcgc ggaatggcga cacagcggca gcggagaagg acgtcaagga
      901 gctcgcaagg ctgcgcgaca cgctgaagac ggcgaagaac gagtattggg cgaccgaggt
      961 cgaggccagc cggctcggcg ctgcggcctg gacggcgctg gcgcaaggca agcccgacga
     1021 agcgctgggt ctcatgcagc gcgccgccga catcgaggac aagaacgaga agcacatcgt
     1081 gaccccgggg cggattatgc ctgcgcgcga gctgctcggc gagatgctgc tggagctgaa
     1141 gcgccccgta gaggcgctga aggagttcga ggcgtcgcac gtgcgcgagc ccgagcgatt
     1201 gcgtggctac tacggcgccg cgcaggcggc cgcgcagagc ggcgaccagg cgaaggccaa
     1261 gcgctatttc gcacggctgg tggaagtggc cggccagggc accgcacgac cggaactggc
     1321 acaggcgcgt gcgttcttgg ccgcaaaccc gtgaccgcag ttgaacgcgt gacacacgcg
     1381 gcctcatgat cagccgaatc ccgcgctccc ggtcgatgag ggcggttttc cggccgcagc
     1441 tcgggcagct cggccatgca gccttggtga cttcgatccg aacatggaaa ccgccgccga
     1501 caaggcctta tccatatcat tttgaagaga ctccaccgaa ttggtggagt ctcttcctat
     1561 caatgacacc cgactgccgc ttgcagttga tggctcgtca tcggtgaagc atgaacgccc
     1621 gcagtaaaag ctgggcgtca tgcgaagaag ttaaagagag agcgtttcct ccaaggggtg
     1681 cccggttttg tctggtatgg cgcgcctgca gggactcgaa ccacggaccc gctgcttaga
     1741 aggagcataa gtagtcgtta ttattaaaga ttttagaggt ttctggtgga cagaaaatgc
     1801 caataattgg atcatgggac gacgggatag gaaccaggcg cgaacagatt tccgagtagc
     1861 cccctatcgg ggataggtca ccatcgacag acggcggcca ataacgggca ccgcactcgc
     1921 ctcctccgga tcaccctgag ggggtggtga ggaaacccgt gggggttaga gtttcccctt
     1981 cgaccccatt gcaattctca aagggatttc ggctagtcga agtccctttt tttatgaaaa
     2041 cgggcagagt ggatcaacag ggactcgcgg gcttcatggt ggcgtttcgc ctgctcctgc
     2101 cgcggagcca attcttcggt tagacgcaaa atgaacagaa ctcgaatcga tcatataacg
     2161 agaagcaacg tatgaatagg attcatacta tacagggagc ccgatcggtt ccaatgcggt
     2221 tacgtgtacg tgtttcggac ttgcgggcct cgcaaagccg caatgggatg acaaatggct
     2281 cggtcgccgg ccgcctatca tagaggtcaa gtgggtcagg gctgccttca tccgcccgca
     2341 caggcggcga tcaacgaaag gtcaattatg gcgaaaaaaa cgctgctttt ggttcttctg
     2401 tgcctcgtat ccagcccact gatggctcaa gagcccagtg ttacgccgct catgtcgaaa
     2461 gatctgacgg accgccccgg caaggaagtt acgatgatca tagtggagta tccccccggc
     2521 gggtcggacc ctgtacatcg gcacaatgcg catgcgttcc tttacgtgct ggagggttcc
     2581 gtcgtgatgc aggtgaaggg cggaaaggaa atcaccctga caccaggtga gagtttttat
     2641 gaaggacccg acgacgttca tatcgttggc cgaaacgcaa gcggtaccaa gccggcgaaa
     2701 ttcctcgtgt tcttaattaa ggacaaagga gctcctgtgc tagtgccagt ggagtgacta
     2761 gggaatgagc gccgtgccac ctctgaagag gaactacaac ctactaaact ctccgagaaa
     2821 ggtctcggac ttcactcgcc cgttgcgggc gccctgcgaa aggtcaactt atgaaaatcg
     2881 tagtcattgg cggcagtggt cttatcggaa caaaactcgt gaacaacctt cgtgaacacg
     2941 gccatgaagc ggtggcagca tcgccagctt caggagtcga tacgcttacc ggtgcaggac
     3001 tcgccgaagc actgacgggc acccaggttg tcgtcgatgt aagcaactcg ccttcctttg
     3061 aagacgcagc ggtgctgaag ttcttcgaaa cctcaacccg caacctcctt tccgcagaag
     3121 cggccgcagg tgtaagacat cacgttgctc tgtcggtcgt cggcgcggac cgtattcccg
     3181 acagcggtta catgcgcgcc aagattgctc aggagaaatt gatcaaggcc gcgacggtgc
     3241 cctacacaat tctccgcgcg acgcagttct tcgagttcat cggcagaatc gccgatggga
     3301 gcaccgacgg caacacggtc cgcctgacac ctgccctcat gcagcccgtc gcggccgatg
     3361 acgtcgccgc cgaactcgac cgcgtcgcgt tgggtgagcc ggtagatggc atcgtcgagt
     3421 tggccggacc cgaaccgatc cggctcgacg aacttgcccg gcgactcctg agcgcgaaac
     3481 acgacgcgcg acaggtaacc gccgatgttc acgcgcgcta cttcggcgca gaagtgaacg
     3541 atcagtcgct cacgcccggc gataacccgc gcgtcggccc aactcgcttc gacgaatggc
     3601 tcgaccgcct tgcggcctag cagcccgaaa attcgattcg ggaggaaatc ctatgagcac
     3661 gattacagtg aaagatggca ccacgatcta ctacaaagac tggggc
//
LOCUS       DATIFQ010000047         3530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1737508, whole genome shotgun sequence.
ACCESSION   DATIFQ010000047 DATIFQ010000000
VERSION     DATIFQ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737508"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VLE22_02170"
     CDS             <1..1084
                     /locus_tag="VLE22_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HSB13233.1"
                     /translation="PDERPSFAPAGLPWFQERRRELDLMWENLVDEAGAIVLVNADPG
                     SGKTSLAQQFAHAARGHFRDILWVACGARSRPSCVSDLGRQLGVALEGSTENVLDRIR
                     RTLQEHRLLVVFDDLTEEPPIVTPPDGRASVLITTRSSEFRVPPHARVMQIESTIPPV
                     LPELPEDSAEARLLHAISVCRPHGFPLDFAAGVAGLDENEAQDACKRLVDGRWVDPFD
                     DAGKRFRIGSGAVAIRRLGAEHEKFRRRHADALKEAMSGWRVNASQCTELLPEFEAAF
                     DWAIQSDWPRANSLADRAFPFLCDQQRLPEAIQIGRRVIEAAEARQDTPIAERWTWEL
                     SWIREGDGGVRRLPNGGDQLPLFDLG"
     gene            complement(1101..2636)
                     /locus_tag="VLE22_02175"
     CDS             complement(1101..2636)
                     /locus_tag="VLE22_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB13234.1"
                     /translation="MLLLLRLLFVAALVSVTASAKTYYVDSAAGNDANDGLAEARAWK
                     TLDKVNGTTFVASDRILFKAGSTYTGQLRPRGSGKEGSPIVVDMYSSGEKPLIAAEGK
                     FKEALLIENQDFWEVNNLQFTNTGAKREKFRYGVRVRAWDYGTMRHIHLKNLYVHDVN
                     GSLVKKDEGEGHGITWENGGGKVQSRFDGLLIEGCHLVRTDRNGICGYVGYRAAKARA
                     LPSLNVVIRKNLLEDIGGDAIKVWGCDRALVEHNVVRGARTRCDDYAAGIWPWASDNT
                     LIQFNEVSGMKGMKDGQAFDSDAYTTNTTFQYNYTHDNEGGFMLVCCFENTGTVIRYN
                     ISQNDRERLFHMAGSNENIEIYNNVFHVGKGIDIHLFLWTPGQGGWTRNARIFNNIFY
                     VEGTGQNSSGERRKPVDDGTYISRPGFGGATKIVFDRNVLYGNFPDVPKDWKTMVADP
                     KLMSPGSGRSGLASLDGYKLQNSSPAIGAGISAKNSGGRDFWGNPVPEGKNPSIGAHE
                     PKR"
     gene            complement(2667..>3530)
                     /locus_tag="VLE22_02180"
     CDS             complement(2667..>3530)
                     /locus_tag="VLE22_02180"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSB13235.1"
                     /translation="STNTVLHALALAHSAGIEYDLNRLNELSDVTPNICKVSPSRPDV
                     HVEDVHRTGGIGAILKEIAAHASTPLDLDVPTVIGTLRDYVEAAPEADGDVIRQTPNA
                     FSQTGGLAVLFGNLAPRGSVVKTAGVDAAMTEYEGAARVYESQEDALSGILGGEVKDG
                     DVVVIRYEGPRGGPGMQEMLSPTSAIVGAGIRAALITDGRFSGGTRGLCIGHVAPEAA
                     AGGPIAAVRNGDRIYINAKTRRLELKVSDEEIRQKLAALPAFEPKVKRGWLARYSLHV
                     TSADTGAVFEI"
BASE COUNT          589 a         1093 c         1127 g          721 t
ORIGIN      
        1 tccggatgag cgtccttctt tcgctccggc tggcctgccg tggtttcagg agcggcgccg
       61 ggagctggat ctcatgtggg agaacctggt ggatgaagcc ggcgccatcg ttttggtcaa
      121 cgccgatcct ggaagcggca agacgagtct tgctcagcag tttgcgcacg ccgcgcgcgg
      181 tcacttccgc gacattctgt gggtcgcgtg cggcgccaga tcgcggccgt cctgcgttag
      241 tgacctaggg cgtcagctcg gtgttgcgct ggaaggctcg accgagaacg tactggatag
      301 gattcgccgg acactccagg agcaccgatt actggttgtc ttcgatgatc tgaccgagga
      361 gccgcccatc gtgacaccac ccgacgggcg tgcttccgtg ctgatcacca ctcgttcgag
      421 cgagttccgg gtgccgccgc acgctcgcgt catgcagatc gagagcacaa ttcctccagt
      481 gctaccggag ctgcccgagg attctgcaga ggcccggctt ttacacgcca tctcggtctg
      541 caggccccac ggatttccgc ttgactttgc ggctggtgtt gcaggactcg atgaaaatga
      601 ggctcaagat gcgtgcaaac gcctcgtgga cggacggtgg gtcgatccct tcgatgacgc
      661 gggtaagcgt tttcgaattg gctccggcgc agtggcaata agacggctgg gcgcggagca
      721 cgagaagttc cgccgccggc acgcggacgc gctgaaagag gctatgtcag gttggagggt
      781 gaatgcgtcc caatgcacag agctcttgcc tgagttcgag gcggcattcg actgggcgat
      841 ccagtcggat tggcctcggg ccaacagcct ggccgaccgt gcatttccct ttctctgcga
      901 ccagcagcgc ctgcccgagg caatccaaat cggccggcgg gtgattgagg ccgccgaggc
      961 gaggcaggac acgcccattg cggagcgctg gacttgggag ctttcctgga tccgggaagg
     1021 agacggcggc gtacggcggc tgccaaacgg aggcgaccag cttccgttgt tcgatctggg
     1081 ctagtcagac gccggctcat tcagcgcttc ggctcgtggg cgccgatgga cgggttcttc
     1141 ccttcgggca ccgggttgcc ccagaagtca cgtccgccgc tgtttttcgc ggagatacct
     1201 gcgccgatgg cgggcgagct attctgcagc ttgtagccgt cgagtgaggc taggccgctc
     1261 cggccgctgc cgggggacat cagctttgga tcggccacca tcgtcttcca gtccttgggc
     1321 acgtccggga agtttccgta caggacgttc cgatcaaaaa cgatcttcgt cgcgccgccg
     1381 aatcccggcc ggctgatgta ggtgccgtcg tcgacaggct tccgcctctc tccactgctg
     1441 ttctggccgg taccctcaac gtagaagatg ttgttgaaga tccgggcatt ccgggtccat
     1501 ccgccttgac caggggtcca gaggaaaagg tggatgtcga tgcccttccc gacatggaac
     1561 acgttgttgt agatctcgat gttctcgttc gacccggcca tgtggaagag gcgttcccga
     1621 tcgttctggc tgatgttgta ccggatcacg gtgccggtgt tttcgaagca gcaaacgagc
     1681 atgaagccgc cctcgttgtc gtgcgtgtag ttgtactgga aggtggtgtt cgtggtgtag
     1741 gcatcggagt cgaacgcctg tccgtctttc atgcccttca ttccgctgac ctcgttgaac
     1801 tgaatcagcg tgttgtccga ggcccacggc cagatgccgg cggcgtagtc gtcgcagcgc
     1861 gtgcgggcgc cgcgaacaac gttgtgctcc accagcgcgc ggtcacagcc ccagaccttg
     1921 atggcatcac cgccgatatc ctcgagcagg ttcttccgga tgacgacgtt caggctcggc
     1981 aaggcacgcg ccttggccgc cctgtagccg acataaccgc agatgccgtt gcggtcggta
     2041 cgcacgaggt ggcaaccctc gatcagcagc ccgtcgaacc ggctctggac tttaccgccg
     2101 ccgttctccc acgtgatacc gtgcccttcg ccttcgtctt tcttcacgag ggagccgttc
     2161 acgtcgtgca cgtaaaggtt cttgaggtgg atgtgccgca tcgtcccgta atcccacgca
     2221 cggacgcgga cgccgtaccg gaacttctct cgcttcgcac cggtgttcgt gaactggagg
     2281 ttattgacct cccagaaatc ctgattctca atcagcagcg cctctttgaa cttgccttcg
     2341 gcggcgatca ggggcttttc gccagagctg tacatgtcga cgacgatcgg actgccttct
     2401 ttgcctgaac cacgcgggcg gagttgccct gtgtacgtgg atccggcctt gaacaggatg
     2461 cggtcactgg cgacaaaggt cgtgccgttg accttgtcga gggtcttcca tgcccgcgcc
     2521 tccgcaagac catcattggc atcattgccg gcggcgctgt ccacatagta ggtcttggcg
     2581 gaagccgtga ctgagacgag agctgcgacg aagaggagtc gaagaagcag aagcatcgta
     2641 tacctcgcgc tgcttgtgag agattgtcag atttcaaaca ccgcgcccgt gtccgcactg
     2701 gtgacgtgca gcgaatagcg agcgagccag ccgcgcttca ccttaggttc gaaagcgggt
     2761 agcgccgcga gcttttgccg gatctcctca tcgctcacct tcagctcgag ccggcgcgtt
     2821 ttggcattga tgtagatccg gtcgccattg cggacggccg cgatgggccc gccagccgcc
     2881 gcttcgggcg cgacatgccc gatacagagg ccgcgtgtgc cgccggagaa acggccgtcg
     2941 gtgatcagcg cggcacggat tccggccccg acgatggccg acgtcggtga gagcatctcc
     3001 tgcatgccgg gaccgccgcg cgggccttcg taccggatga cgaccacgtc accgtccttt
     3061 acctcgccgc ccagaatgcc ggacagggcg tcctcctgcg attcgtagac gcgcgccgct
     3121 ccttcgtatt ccgtcatggc cgcgtccact cccgccgtct tcacgaccga gccgcgcggg
     3181 gcgagattgc cgaacagcac ggccagtccg ccggtctgcg agaaggcatt cggcgtctgc
     3241 cggatcacat cgccgtccgc ttccggagcc gcctcgacgt agtcgcgcag cgtgccaatc
     3301 accgtcggca cgtcgagatc gagcggcgtg ctcgcgtgag ccgcaatttc ctttaggatg
     3361 gcgccgatcc cgcccgtgcg gtgtacgtct tcgacatgca cgtcgggccg gctgggagag
     3421 accttgcaga tgttcggcgt cacgtcggac agctcgttca accggttgag gtcgtactcg
     3481 atgccggccg agtgcgcgag cgcgagggcg tggagaaccg tattcgtgga
//
LOCUS       DATIFQ010000048         5255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1162680, whole genome shotgun sequence.
ACCESSION   DATIFQ010000048 DATIFQ010000000
VERSION     DATIFQ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162680"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="VLE22_02185"
     CDS             complement(<1..1337)
                     /locus_tag="VLE22_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13236.1"
                     /translation="MKTKILVSVIVSTVLLLVPCAFAQGAKPDRDALVKFQNALKQDG
                     FDVTPGAAEVWDPLGSWCADDLPNARLVNSGPNLVLTVPKEVPKPGEKPDLIPTFRIR
                     HDEAIVLIGRTPPPARYFGFYTFIATRFYPGKEEETEILSTLGDAVNHATIKTTGPTP
                     FDAPVVLIFTPDRGTDAGIRAALRRAGYPGAIINTNVLPASMLNLGHGETADEFQINL
                     RTALFEDPDAGNRYMTDAPRNLHILRVTPRPPAIADPFPMPPLRVRGTGESELRLMNK
                     LGELRTGIIAANAGLYATDFLSAIATYEDYDFLQRAKWADLGTRDAFVLSAGWMMPVS
                     YLNDVITLADDEFLMVYGVNHFATLKTTYMNINFYATGREVNGEPVEEANAFVGGVSD
                     KDLPGTATDYLPAGDPDAKLMYAYKVSRSCGEDEPNCLPLSAPCTRVTLASSTV"
     gene            complement(2808..4106)
                     /locus_tag="VLE22_02190"
     CDS             complement(2808..4106)
                     /locus_tag="VLE22_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13237.1"
                     /translation="MDTFQKALERDGFHVTPGAAAFQNLVEEWCAGTIDSALYANNEP
                     YIQVLVPESVQKPDQLSTNFQLRPDEAVVLIGQTPPAARYFSYTPYIWSKLYPDGKRR
                     LPFATLGDSVNNATIKAIGPTPFNSPVALVFTPDQGTDARIRAALRHADYPSAIINTV
                     VFPSSMLKLGRDETADQIRIVLRNAIWQLQAAGDSYIHNPPLSVFRVTPRTPATENPF
                     PAPPLRIRGTGQTEMNLMNKLDQLREGIIAANPELDATEIVMMPMCYEGYDLIQLGLE
                     MCGDSRDAFYVGAGITEFDKINRITLSDDEFLVLYGVNHVTTGKATYMNTNIYASEEA
                     KLTLGAIDDRDFPDTARPYLPAGDPAAAVMYAYRISRSCGNESNCKQLTVPEGCTRLT
                     LDSNTLLGVFTRTYLEPETKVGPAMPEMLYDRLIKFSPRP"
     gene            4501..4749
                     /locus_tag="VLE22_02195"
     CDS             4501..4749
                     /locus_tag="VLE22_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13238.1"
                     /translation="MSRRRPETTRPQCPSRNRKEAGDVDRDSVLAKRRHDVAIVGDKL
                     LHGPEPRVAAKPVCPGEGAVGIPRRAAREDNESPLFTV"
BASE COUNT          998 a         1549 c         1607 g         1101 t
ORIGIN      
        1 acagtgctgg aagcgagtgt cacgcgcgtg cagggggcgg acaatggcag gcagttgggt
       61 tcgtcttcac cacagctccg cgacaccttg tacgcataca tcagcttcgc atcaggatcc
      121 ccagccggga gatagtccgt ggccgtgccc ggcaagtcct tatcggagac tccgcctacg
      181 aacgcgttcg cctcctcaac gggttctcca ttaacttcgc gcccggtggc atagaaattg
      241 atgttcatgt acgtcgtttt gagggtagcg aagtgattga cgccataaac catcagaaac
      301 tcgtcatcgg ccagcgtgat gacgtcgttc aaataagaca cgggcatcat ccaaccggcg
      361 gacaggacaa atgcatccct ggtgcctaga tccgcccatt ttgcgcgttg caggaagtcg
      421 tagtcctcgt aggtggctat cgctgagaga aaatcggttg catacaaccc cgcattggct
      481 gcgatgatgc ccgtgcggag ttctcccagc ttgttcatca ggcgcaactc actctctccc
      541 gttccgcgga cgcgcagagg gggcatgggg aagggatcgg cgattgccgg agggcgtggc
      601 gtcacccgga ggatatggag attccgcggc gcatctgtca tgtagcgatt gccggcgtcc
      661 ggatcttcaa acagcgcggt gcgcagatta atctggaatt cgtcagcggt ctcgccatgc
      721 ccgaggttca gcatcgatgc cgggagcaca ttggtattga tgatcgcccc cgggtagccg
      781 gcgcgccgca gcgccgcacg gataccggcg tccgtacccc ggtccggggt aaagatcagc
      841 acaacgggag cgtcaaaagg cgtaggtcca gtggttttga tagtggcgtg attgacagcg
      901 tctcccaagg tactcagaat ctctgtttct tcttccttac cggggtagaa tctggtggcg
      961 ataaacgtgt agaagccgaa gtatcttgcg ggtggaggcg tccgcccgat cagcacgatg
     1021 gcttcgtcat gccgaattct gaacgtcggt atcaggtctg gtttctctcc cggtttcggg
     1081 acctctttcg ggaccgtcag gacgagattg ggacctgagt tgaccaaccg agcgttcggg
     1141 aggtcgtcgg cgcaccaact tcccagcggg tcccaaacct ccgcagcgcc gggggtcaca
     1201 tcaaatccgt cttgcttgag tgcattttga aatttgacca aggcgtcacg gtccggtttc
     1261 gctccttgag cgaacgcgca cggtaccagc agtaacacag tagacacgat tacggataca
     1321 aggattttcg tcttcatatc attctcctcc tggaccggta acgctaccga tgttccctgt
     1381 cgggtcaacg actcctcgac atatcccgca cctacgggcc tcgtgggcac catcctgaag
     1441 ggctccgagg acggctacgc tgacaccgtg agcaacaccg acacgttctt cgtgcactta
     1501 ttacctccgt tgcggctcgg acgcctgatc aattcccatt agctcgtcgg tctcacgccg
     1561 ggcgccggct cgacgcggct gggatcgaaa gggagctccg ggggacgttg atcaggagca
     1621 gcctgccacg tcgtccacgg caagaagggg gcatcctgtc gcgctgcccg gtcctcctga
     1681 ttcgaaagga ccgtgctgct ggaaaatgct ttgttcgagt cctccgccgc tcacccgttc
     1741 gatcccgttc ttcccggcgc aaatctatgg aaagcggcgg aattctagaa atcgcccgcg
     1801 ccgccggatt ccggcttttg cgggatcttc cgctgagaac tcgcccgacg ccgcgcgttc
     1861 cgcgcacggc ccgcccgtct tagtccttgc agagagcgcg cggaggctcg atccctgaaa
     1921 cgttctggcg agcatgaaca gttgctgccc gactacttct ccgaggtgga actcgcagat
     1981 cccgactttt gcgatcgact ctccgagacg agaggcgaca cagaaccggc cgactttcgg
     2041 ctgacctgaa tgaaaaggag tcaggcgagt ccgcccaact cccttgattt catggttgcg
     2101 ggggaaggat ttaccgccgg cccgcggccg gcgttctact ccgatgttgc ggcttccgcc
     2161 gccatcggag tagttaaacc ttccgcggct tcgccctttc tccggcttca aatctttgcc
     2221 gccatcggtc aggaacagca acgccgagag ttatctctcg gcgtcgaatg aatcttggtt
     2281 gcggggccgg gatttgaacc caggaccttt cggttatgag gactctccga tcgtcgactc
     2341 cagtcgacaa aacccgaaag gacgcgggtt cgtcggttcc ccgtgttgtc ggttttcgga
     2401 cggtgacggc tgggttggca gcgacttagc ggcgaccacc ggagcgtcgc ctaaaggtct
     2461 tactcgggtc agccctccga tcggagagcc acgcgacggc tgtccggagc aacctttcgg
     2521 acgcaaagaa agaggcccct tgcgggacct cgtgttgaac gagcgcgttc gcgcccggtc
     2581 tgacttatga gtgaagagtc gtggcggtga cgtcgtaggt ctcaccgtcg gggttccgac
     2641 gatcacgggg ttgagcttcg cgagaatctt gggatacgtt gaagtctgat aggcctcggc
     2701 gttctttcgg ttgtcccaaa ggctgacgct gacggcggga ttcgaacccg cccgcccggt
     2761 gctttcgcta gtggtcctcc tcagtgctgt gccccaatcc ctcacgatca aggccgcggc
     2821 gagaacttga ttaggcggtc atagagcatc tccggcatgg ccggcccgac cttggtctcc
     2881 ggctccaggt atgtgcgggt aaagacgccc aggagcgtgt tggaatcgag cgttaggcgt
     2941 gtgcagcctt cggggacagt gagttgcttg cagttgcttt cgtttccaca gcttcgcgaa
     3001 atcctatacg cgtacataac ggccgcagca ggatcgcccg ccgggagata aggcctggca
     3061 gtgtccggga aatcacggtc gtctatggcg cccaatgtca gcttcgcctc ctcgctggca
     3121 tagatattgg tgttcatata cgtggcctta ccggtggtga catggttgac gccgtaaagc
     3181 acaaggaact cgtcatcgga gagcgtgatc cggttgatct tgtcgaactc agtaataccg
     3241 gcgcctacgt aaaacgcatc ccggctgtcc ccgcacatct cgagaccgag ctggatcaag
     3301 tcatagcctt cgtaacacat cggcatcatc acgatctcgg tggcatccag ctccggattg
     3361 gccgcgatga tgccttcgcg gagttggtcc agcttattca tcagattcat ctcggtctgt
     3421 ccagttccgc gaatgcgcag aggtggcgcg gggaacggat tctcggttgc cggggtgcgc
     3481 ggcgtcacgc gaaagacact gagcggagga ttgtgaatat aggagtcgcc ggccgcctga
     3541 agctgccaga tagcattgcg caggacgatc ctgatctggt cggcggtctc atctcggccg
     3601 agcttcagca tcgatgaagg gaaaacgacg gtattgatga tcgccgacgg atagtcagca
     3661 tgcctcagcg ccgcacgaat gcgggcgtcc gtcccctggt caggggtgaa gaccagcgcc
     3721 acgggggaat tgaagggcgt cggcccgatt gctttgatgg tggcattatt cacggagtct
     3781 cccagggtag caaaaggcaa gcgcctcttt ccgtcggggt agagctttga ccagatatag
     3841 ggtgtgtagc tgaagtatct tgcagccgga ggcgtctggc cgatcaggac gaccgcctca
     3901 tcgggccgga gctggaagtt agtgctcaat tgatccggct tctgcaccga ttctgggacc
     3961 agcacctgaa tatagggctc gttattggcg tagagggcgc tgtcgattgt tccggcgcac
     4021 cactcttcca cgagattctg aaaggcggcc gcgccgggag tcacatgaaa tccgtcacgt
     4081 tcgagtgctt tttgaaacgt gtccatcgcg ttagggtttc gtcccgcgtg ttgagcgaac
     4141 gcgcagggta ccagcagtaa cactgcagac atgattgcgg atactagtat ttctgtcctc
     4201 atatcctctt cctcctagac cggtaacgcg acctatgtcg gcctgtcggt caaggacgcc
     4261 tcgacatatc gcgcacctac gggcctcgcg gacaccatct ccaacgctac ctcccttgct
     4321 gctcgggcgc ctgatccgtt cccatcactc gtcggtctca caccggacac cggcgcgacg
     4381 cgcttgcgga cagtctgccc gttgcgccgc acctcgtcct ggatcgagac gggcacgcac
     4441 cagacgcggg tcgtctcttt cgtcgcgatg tgcgatgtgc cgaaggtgtc ggcaaattcg
     4501 atgagccgac ggcgacccga gactacgcga ccacagtgcc cgtctcgaaa tagaaaggag
     4561 gctggggacg ttgatcggga ttccgtcctc gcgaagcgcc ggcacgacgt ggcgattgtc
     4621 ggcgacaagc ttctgcacgg tccggagcca cgagtcgccg cgaagcccgt ttgcccgggc
     4681 gagggtgcgg tggggatccc gagacgggcg gcgagggaag acaacgagtc gccgctcttc
     4741 acggtgtgag tgatcgcgtt cccgacgatg atccccggag tcggcggctg ctccgggact
     4801 tgcgggaacc gcacggcaag gagaagaacg acgagctgca ggatcggatg ggaggtgcgg
     4861 ccggcgttct actccgatgt cgcggcttcc gccgccatcg aagtagtcaa accttgggcg
     4921 ccggcagagc cggcgcagcc tactgccgtg ggtctgcctt cggtggccgg ctcccgcggc
     4981 ttttctccgg cttcaaatcc ctgccgccac cggtcaggaa cagcaacgcc gagagtcacc
     5041 tctcggcgtc caatgagtct tggttgcggg ggaaggattt gaaccttcga cctttgggtt
     5101 atgagaactc ctttaccgtc tgctcagagc gaaaaaaccc gtaaagacgc gaattctctc
     5161 ccggcacgag tcgacggttt tcggcaggtg tagacgcgaa accggccgac tttcgcccga
     5221 ccggatgccg gcgttcgacc gttcaggaag ctcaa
//
LOCUS       DATIFQ010000049         2102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1163184, whole genome shotgun sequence.
ACCESSION   DATIFQ010000049 DATIFQ010000000
VERSION     DATIFQ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163184"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..573
                     /locus_tag="VLE22_02200"
     CDS             256..573
                     /locus_tag="VLE22_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13239.1"
                     /translation="MRRTFCLFAATAIALPSAAVPAPPAPGSISPNHETGQQVGGENG
                     LECDEGGTPPSQSGREPGPGSPFQEDSVSGSHYAGNQDGINNKNTASVSQYDIACFKG
                     PGQ"
     gene            679..1725
                     /locus_tag="VLE22_02205"
     CDS             679..1725
                     /locus_tag="VLE22_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HSB13240.1"
                     /translation="MQQCSLLSLATAVPPHVIEQQDAKACAREAFGGKKALFDRLAGV
                     FDNAGIARRHIVAPQEWYMSAHGWHDRNAVYLEAAESLFVEAASDAIEKAGLAPEQID
                     GVVTVSTTGIATPSLEARVGPLMGLRDDVRRVPMFGLGCAGGVNGLATTSRLAASEPG
                     TNWLFVTVETCSISIRLDSDDPAAVVATALFGDGAAAAVVTSGEHSIARIMGGAEKLF
                     PDTLRIMGWDVEDPGLAVVFDRAIPPFIEKNLASTVDEMCAKFDIDRSDIDRLCCHPG
                     GVKVIDAIETALHLNQGELNIEREVLRDFGNMSAPTVMFVLDRLLKQGLPDRVLMTAF
                     GPGFTCAGLLLEAA"
     gene            1725..>2102
                     /locus_tag="VLE22_02210"
     CDS             1725..>2102
                     /locus_tag="VLE22_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HSB13241.1"
                     /translation="MSPLWLAVVILGFVTLQRLIELRLADSNSRKLLSEGAVEFGRDH
                     YAFIVGLHMIWIAALWWWAPGQPIDFALLVLFALLQLGRVWVIATLGDRWTTRIIVMP
                     GAPLVRTGPYRFLRHPNYLIVTLE"
BASE COUNT          364 a          668 c          665 g          405 t
ORIGIN      
        1 cgccgcccgc gagttcggtg gcgtctccat ccatctgatc tggtcaggcg gcggaccggg
       61 aaaggatttt taccggggcc gataccacga ccggaatgcg ctcatcgtgg gtctcagctg
      121 tcttctttga cagccgattg aacccttcgg ccgcctgggc gcactaacgc cctgctgtga
      181 cggcgatccg ctcatccgag catcgctgtc ggcgtgtgtc gttcctgagc gggggcagaa
      241 caaggagctt cgatcatgag acgaacattc tgtctctttg ccgcgacggc aatcgcgctg
      301 ccgtccgctg cggttccggc cccaccggcc cccggcagca tttcaccgaa ccatgaaacg
      361 ggtcagcaag tgggaggcga gaacggcctc gaatgcgacg agggcggcac acctcctagc
      421 cagtcgggcc gggagcctgg tcctgggtcc cccttccagg aggacagcgt ttccggttcg
      481 cactatgctg gcaatcagga cggcatcaac aacaagaaca ccgcgtccgt ttcgcaatac
      541 gacattgcct gcttcaaggg gcctggtcag tagtgaccgg ccaaggctgg cccgcctcgc
      601 cctcgggcgg gccagttgct tcttaggcat tcggcttgtg cagcgtgact gatgatataa
      661 gccggaaggc ccaagccgat gcaacaatgc agcttactct ctctcgcgac tgcggtccca
      721 ccgcacgtaa tcgagcagca agacgcgaag gcgtgtgccc gcgaagcctt cggtgggaag
      781 aaggccttgt ttgaccgcct cgcgggggtc ttcgacaatg ccggcattgc ccggcgccac
      841 atcgttgctc cgcaggaatg gtacatgtcc gcccacggct ggcatgatcg caatgccgtc
      901 tatctggaag ccgccgagag cttgttcgtc gaggcggcga gcgatgcgat agagaaagca
      961 gggctggcgc ccgaacagat cgacggcgtc gtcaccgttt ccaccaccgg aatcgcgact
     1021 ccgagccttg aggcgcgcgt gggaccgctc atggggcttc gggatgacgt ccgccgggtc
     1081 ccgatgttcg gcctcggctg cgcgggcggg gtcaacggcc tagcgacgac ctcgcggctt
     1141 gccgcctcgg agccggggac gaactggctg ttcgtgaccg tcgagacctg ctccatctcc
     1201 atccgcctcg acagcgacga ccccgctgcg gtggtcgcga ctgccctgtt cggtgacgga
     1261 gcggccgcag ccgtcgtcac cagcggcgag cacagcatcg cccggatcat ggggggcgcc
     1321 gagaagctct ttcccgatac gctcaggatc atgggctggg atgtcgaaga cccgggtctg
     1381 gccgttgtgt tcgatcgcgc gatcccgccg tttatcgaaa agaatctcgc aagcaccgtc
     1441 gacgagatgt gcgccaagtt tgacatcgac cgcagcgaca ttgaccgcct gtgctgccat
     1501 cccggcggag tgaaggtgat cgacgcgatc gagacggctc tgcatctcaa tcagggcgag
     1561 ctcaacatcg agcgcgaggt gcttcgcgat ttcggcaata tgagtgcgcc gaccgtgatg
     1621 ttcgtgctcg atcgtctgct gaagcaggga cttccggacc gagtgctgat gacagccttc
     1681 gggccaggtt tcacctgtgc cggactcctg ttggaagccg cctgatgagc ccgctctggc
     1741 tggcggtagt cattctcggc ttcgtaaccc tgcaaaggct catcgaactc cggctcgccg
     1801 acagcaacag caggaagctg ctctccgagg gtgctgtcga attcggccgc gaccactatg
     1861 cgttcatcgt cggtctgcac atgatctgga tcgcggcact ctggtggtgg gcgccagggc
     1921 agccgatcga ctttgcactg ctcgtcctgt tcgcactgct ccagctcgga cgcgtgtggg
     1981 tgatcgccac tttgggggat cgatggacga cgcgtatcat cgtgatgccc ggcgcgccac
     2041 ttgtccggac cggcccctat cgtttcctca ggcacccgaa ctatctcatc gtgaccctgg
     2101 aa
//
LOCUS       DATIFQ010000050        20121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_397411, whole genome shotgun sequence.
ACCESSION   DATIFQ010000050 DATIFQ010000000
VERSION     DATIFQ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 20121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20121
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397411"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="VLE22_02215"
     CDS             complement(<1..1473)
                     /locus_tag="VLE22_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13242.1"
                     /translation="MTRFVLLSLPIMLLAQGAELPVRQVILYKHGVGYFERSGEVAAG
                     EAARLDFKASEMNDVLKSLTIEDKRGNSISGVRYDSSEPLSGKLAEFPFQLGERQALS
                     TLIDSLKGARIDLRTGPETISGAVVGARLVPATQEHPEREQITLLLDSGALRTFDLST
                     ATEVRFRDAELQNQIRDYLAAMTGARSREQRSVYIDSADSAARQLFASYVIPVPVWKS
                     SYRLVFGATGEPLLEGWAIIDNVTSYDWSKVRLSLVSGRPISFVSKLYEPRYISRPTA
                     ELPEERAQAPVVHEGAIGGAVEQLAAAPPAPAKRAFAKAGEMRMLDLAASAPMAEVAP
                     REEIASTIAASAESRELGELFEYSFSAPVTVRKGESAMLPFLQQKIAARKLLIYADQR
                     SQHPTNAAEITNNTGKTLDGGPVTVFDSAAYGGEALIETLKASDKRLISYGVDLGTRI
                     TTQFDSGRQILREVHFRRGVLTSRHAVQETRTYTIRNVDQK"
     gene            1703..3025
                     /locus_tag="VLE22_02220"
     CDS             1703..3025
                     /locus_tag="VLE22_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13243.1"
                     /translation="MRTRILLVLTTALAGAAFAQRADYEGPTILSRGIGPVLGGGGDL
                     LRIRPYLSVTGVYDTGLTPVSVNSQGQIPKEDGYGGELGFGALGYHSWRDTVLGLDYR
                     GDVRHYSQQSYYDGSDHTLSLGVTHQLSRRVVLSLREAAGTYSRSFGPMGGLGYFDPS
                     FADIPQNELFDGRTHYLTTMGDLTLQKGPRLSFNMGGSGMVVRRRSRSLIGVTGWTAR
                     GDTAYRVARNQVIGVDYGFHHFDYTSAFGASDIHTLGLNWTARIGRNWSLGLRAGASR
                     VETLGLRRVTLDPIIAAILGQSSGIETFYRVNYVPSANARLTRTFRRSSVSFEYDRGT
                     GSGNGIYLTSQQESFGLNASYTASRRWNVGASAGYDNFRSLGQTLGKYESYHGGGGVT
                     FQMTSVLHFVARFDGRHYDIESTDFKRTHYRASLGIALSPGDMPLSLW"
     gene            3029..3415
                     /locus_tag="VLE22_02225"
     CDS             3029..3415
                     /locus_tag="VLE22_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB13244.1"
                     /translation="MPETDPRKRILVVDDEPALLKLLERYLTRSGYRVEACGSAQQAL
                     ERYGADPGAYSLVIADLRMPGMSGEELLEMLIRRNPSIRIVVCSGYPFNHLERHPGIE
                     GQIRFVQKPFLPSMLDHAIRELLGSS"
     gene            complement(3412..3996)
                     /locus_tag="VLE22_02230"
     CDS             complement(3412..3996)
                     /locus_tag="VLE22_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13245.1"
                     /translation="MADRLYLSYWIRGYSGQTMLRHFEKLLRKFPFSPNSPAPVELRI
                     YVQQFSEPPLIERVFDRLTKDLDDVIAASREFENADSGYIVEGYWDLWRYDPEWKLTP
                     APVSLACYGPLFENDLGDHLRIEFGNDADFLPQPETPGSSRKVESNIRGLLRLVHELD
                     DTLPTENRRLWSESGENFADRLAEALSEHPRAGS"
     gene            complement(4039..4800)
                     /locus_tag="VLE22_02235"
     CDS             complement(4039..4800)
                     /locus_tag="VLE22_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSB13246.1"
                     /translation="MTALLCMLLLQERSRLEAFLAAPEADPWILATALGVSFMLGAAH
                     ALTPGHGKSIVAAYLVGSRGRLTDAVFLGAVVTATHTASVFVLGIVTLYASQRVSLDR
                     VYPWLALASGVLVALIGAWLLSERLTARTHSHDHDHVHHHHAHNHHHDPPTRGSLLSL
                     GVSGGLVPCPEALVVLMISISLRRIGFGLAILVAFSLGLAAVLISIGVAMVLAAPALK
                     RLSGEGPLVRALPVTSAVIVTLLGLVIALQAARSF"
     gene            complement(4797..6068)
                     /locus_tag="VLE22_02240"
     CDS             complement(4797..6068)
                     /locus_tag="VLE22_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB13247.1"
                     /translation="MIRSRRGFLRSLPGAAVLPASWVSFTRTLEAAPGIPDATNEAYW
                     RMVQRQFPLEDGLIFLHAANVCPASRPVLDRYVEFLRDFEANPSFQNREKYEPLQEQL
                     RTKLAAVLGASADEIAITRNTSEGNNVVVKGIDLKAGDEVLITDHNHPSNEASWKMRA
                     KREGVVVRSVPVTVPAPGREQLLDGIVRAVTPRTKVIAVTHVTNTTGILYPVKELADF
                     ARSRGIWMHLDGAQTFGALDVNVRAIGCDSYAASAHKWAMGPLEAGVLYVRSERIPEL
                     WPSIVTAGWSDRLVGARKFEVLGQRDNPRVVAFDAAADFLQLLGMKNVEARMRLLANA
                     LKQKLREMPRVQLLTNLEPQLSGGVVKFRIRDADTRRAYDALWSRHRISGAMTPATTA
                     SNQEGIRLCPHIYNSIEQIDRTAAAVQELAG"
     gene            complement(6136..7068)
                     /locus_tag="VLE22_02245"
     CDS             complement(6136..7068)
                     /locus_tag="VLE22_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13248.1"
                     /translation="MKIVQIGILAALIACGVLLFLVLKGRQSEQAAVTTAETSEQAAA
                     PSSQAPAPPPAEQAQQLATETAAPAPSRAKPAPSVKPAAQKTVTAKSQTTAPEPKAEQ
                     TPAQSSQPAAASNVAEQKAPAPMAVTPPPPPPEPRKVTVAAGTLLSARLAETLDSSRM
                     KLGDTFTANLDQPLVVDGLVIAERGARLEGKVAEIQESGRVKGLAAMAIHLTKLHTSD
                     GQELAIETDLFHKQAPTSRGEDVKKVGIGAGIGAAIGAIAGGGKGAGIGAAVGGAAGA
                     GTAAATRGKPAVIPVETRIDFRLSGPLTVTEKLK"
     gene            7160..8173
                     /gene="dusB"
                     /locus_tag="VLE22_02250"
     CDS             7160..8173
                     /gene="dusB"
                     /locus_tag="VLE22_02250"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HSB13249.1"
                     /translation="MREFPPKLSIGKVEIAPATVLAPMAGVTDTVFRRFIRGLGGCGL
                     IMTEFTSSHGVVASSVRRKPTRTFHYLVFEPDEHPISAQLFGADPDVMANAARVCQDL
                     GFDIVDINLGCPVKKVVRCNGGSGLLRDLPLVERILRAVRAAITTPLTVKFRAGWNDR
                     ELVSVPLARIAEDCGLDAIALHPRTREQGYSGKADWDRIAEVKAAVRIPVIGNGDVIS
                     PEDAFRMVHETGCDAVMIGRAASYNPWIFRQIDQYREKGRYDCPTERDRYDMMRTYYA
                     RLIDRGEDDTVGKMKQFATYFTHGVRNGSRLRQEVHLAHAATEIVERVDRFFVHELEA
                     CPA"
     gene            8178..8615
                     /gene="rnhA"
                     /locus_tag="VLE22_02255"
     CDS             8178..8615
                     /gene="rnhA"
                     /locus_tag="VLE22_02255"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HSB13250.1"
                     /translation="MKKVQIITDGACLGNPGPGGWAAILRHARHKRELSGSEPHTTNN
                     RMELTAAVRGLAALKESCEVEVITDSEYLKNGITEWLPRWKRNGWKTASKDPVKNQDL
                     WEQLDALTERHKTRWVWTKGHANHEDNNRADELASAAARGVSR"
     gene            8612..9247
                     /locus_tag="VLE22_02260"
     CDS             8612..9247
                     /locus_tag="VLE22_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13251.1"
                     /translation="MRTLLLFVLAAGGALAQPLSLGVKAGVPLNNAFKIADASTYLSD
                     RQPYVIGPMAELHLPFRLSVEFDALYRRVKYSSEGVSGSEPSVPLGTSMTTKGRSFEF
                     PLLLKYHVAEGIVRPYLGAGLVWRSIGGLKQTFLPAGGTGLTETDEPAELRDSSGGGV
                     VFSGGLELRVPFLRVSPELRYTRWGTTSFRTAVGDFRSQVNQAEFLIGITF"
     gene            9279..9935
                     /gene="nadD"
                     /locus_tag="VLE22_02265"
     CDS             9279..9935
                     /gene="nadD"
                     /locus_tag="VLE22_02265"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HSB13252.1"
                     /translation="MIWRMKRKRRAMEEDITIRSRVAIFGGTFDPIHSAHLIVAREAL
                     ELYGLSQVLFVVAGNPPHKPQTTSAPYKHRMRMVELACEATSEFVPSRLEETEEVSYS
                     ITTIEKVKNLQGPEGKLYFLIGADAFAEIRTWYRWGDVIRGVEFIVVTRPGHQYKVPP
                     GALVQRLETLALPVSSSEIRARLAAGDDPPEVPRPVLAYIREHGLYGYPRPGRVSAGL
                     "
     gene            complement(9989..11794)
                     /locus_tag="VLE22_02270"
     CDS             complement(9989..11794)
                     /locus_tag="VLE22_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13253.1"
                     /translation="MTDAPGSSRSDPKAEYIRRRDAKLAVAGRHERLHVRFGNLRLAV
                     AVMAALIGWLALVRDTLTPWWLLAPAACFVVLAILHDRVLRARSHELRAAAFYDQGLA
                     RLSDRWAGTGEAGDRFSSKEHLYSDDLDLFGKGGLFELLSIARTRAGEETLARWLLSP
                     APLDALHARHAAVDELRTRLDLREDLAVLGEEVRAGLHSEPLAAWGDQPPILDSPRAR
                     IAAATLALAAVAALVLWIETGHRIAFFAVLVLEAAFGLHYRKRVERAVHAAELPSHDL
                     ALLSEVLARLEREKFHSPMLAELRARLDTETQSPSRSIARLNRLMELLDSRENVVLRV
                     FGPPLLWTTQLAFAVENWRKHHGPAVRRWLEAVGEIEALSSLSGYSWEHPADPFPELT
                     EDGPRFDGEGLGHPLIPEARCVRNDLRLGSDIRMLVVSGSNMSGKSTLLRTVGVNAVL
                     AMAGAPVRATRLRLSELLVGASIRVVDSLQGGTSRFYAEIKRLRRLVDLCGGQRPVLF
                     LLDELLHGTNSHDRRTGSEAVVRGLVERGAIGLVTTHDLALAHIAEVLSPPGSNVHFE
                     DNIEDGRITFDYILRPGVVRKSNALELMRSVGLDV"
     gene            complement(11817..13121)
                     /locus_tag="VLE22_02275"
     CDS             complement(11817..13121)
                     /locus_tag="VLE22_02275"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HSB13254.1"
                     /translation="MTAPAVALLLLFAAQASGPAPSIPKRARALIPRLERALTENIAV
                     FWYPRSLDRKHGGYIIAFDPKGEPKREYTKGLVTQARTLWLFARLARGGFKGPGWGNR
                     EMLEAADWGYRFLREKMWDHEHGGFYWEVDATGDRQLRPRKMIYGQAFALYALSEYYL
                     ACRRKEVLDFAEQFFRLLDAKAHDRTYGGYIEAFEEDWTPLPDGETTYMRPAGLKTMN
                     SHLHLMEAMTVFAEASELEKARERLLELITIESNTVVRKDAGACTDKYTRQWEPLMDG
                     DNARVSPGHDVENIWLLMEANRAAGIPDHPLHDLYRTLFAHALKFGNDEYRGGFYESG
                     LPGLLADRRDKIWWVQAEALVAALRMFDFTGDPKYFEVFEKTWEFVDQHQIDWKSGEW
                     HAIVTPDGPADADKGHLWKAGYHNGRALIECLKVLRKVAEEP"
     gene            complement(13118..13486)
                     /locus_tag="VLE22_02280"
     CDS             complement(13118..13486)
                     /locus_tag="VLE22_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13255.1"
                     /translation="MATKTIGIAVALILLGVAGYVFSGFVSVTALIPAFFGIVLLALG
                     ILARDDKKRKMAMHIAAAVGLLGFLGSVRGLMKLPALLQGEPLERPAAVLAQSIMALF
                     TAFFVGLCVKSFIDARRSRP"
     gene            complement(13508..16804)
                     /locus_tag="VLE22_02285"
     CDS             complement(13508..16804)
                     /locus_tag="VLE22_02285"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSB13256.1"
                     /translation="MRKQDWNRRQFLGTSVAAATAATAARTPAHAQDVGMGARKGSSK
                     RLETPYTLEIETPHVKWAKPLLGGPIRLLAVSSVDEGRTVVELAQRLSLDLTTVSIDP
                     AWDVNKWTMCFGRDYGARAERGDLNLVYSYLEQELTSTKQFDAILLPLSHGWKALTSA
                     SREALARRVKEGSGLVLIRPFSSEICPLEPTTAPPESEDELEVPREPGKTESSPWHRK
                     GEHYITRAVPVESFPFQHLDQYICRAAAGAEVLIESETGHAILAAHTYGSGRVAAFGY
                     RNAGVSWYMPQTARNDFVDTYWEYFYSLLCRALIYVAKREPAAIPDWEVPSTEWRVRD
                     LRSRIRDSGQGPTKQSQTLEGGRYFLEQQNGAEWRITVLDVQQSDRVEELKASKEIIS
                     EGDAVDVTWKSTRRARIELIDGLGRVIARSSGEDKARLKAGKPLVHSGWVRATAGSAV
                     AQIPVRFAASSREWKDYEILLPWFGPRSYQPWIPTVDEQLRSIGITTLARPERNFKIM
                     ASVHPLAFGIYWYRRDGYLKRKKAFAETGDKKYLTREVVLQSPEFEKSVKASLEKNVR
                     PVAALKPWAYYLADESSLTCYTDPFDVDWAPEAIAGLRQWLRKEYGSLEKLNSAWGTS
                     FQEWDSVVPMTTEEAQKHGNFAPWSDHRVYMEQEFVQAFAKAREWLREIDPEGRPSIS
                     GTQIPTAHNGCNWYEIDQQLEYIQPYSGGSQDAMHYLFNPKLQITGFTGYGLVGDEAK
                     HQQWQRLFYGHTGASIFWYYTLMNPDLTLSEQGAALADAFGRLQSGIGRIFMNSKVRE
                     DGVAVHFSMASIRGAWITDGKLSADMGNVMRSSKNFAELNKLRNAWVEDLERQGLQLR
                     FLATPQIEAGDLKNYRVLILPYSIALSDREIQEIERFMDRGGVVYADEQTGKMDERCR
                     WRKAVLWQHGRKGLIRRGPGDIELESAVPTDGKFLVTVRDFGGSRLIGLLPKEKAIYK
                     LPPMEGVIYDLIRGETARGTLEATPGTPVLLLARQSAITRLDMDANLNLRLLDAAGAP
                     VDRSVVHVEVFNPEDRLVREYCMNVDIVNGAGRIEIPFAMSDARGAWRIRVRDAISGL
                     GAERTLTPKA"
     gene            complement(16900..17343)
                     /locus_tag="VLE22_02290"
     CDS             complement(16900..17343)
                     /locus_tag="VLE22_02290"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSB13257.1"
                     /translation="MKQNRRSLLKVAAGVAAAAGAAKAAGGPVKKVHYRGEKPKKTPL
                     FSGAVSYGNLLFIAGKGAHFQGDITAHTKHVLDEVQKELENAGSSMDKVLKCNVYLND
                     LKDYKAMNEAFLGRFGSEPPVRTTIAAAGGIPGDSLVEIDVIAYI"
     gene            17384..18511
                     /locus_tag="VLE22_02295"
     CDS             17384..18511
                     /locus_tag="VLE22_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB13258.1"
                     /translation="MRRRAFLGIPAASAFSFSADSAPVPRYKVVSAYKSQAGQGMPGL
                     FPGSVVSVHSNTSIDETTEKVNAPAVAQMVAEGMKTLCGVKDPRDAWRRFFTPQDVVG
                     IKVNCSGAPGIMSTPAVVAEIVRNLVAVGVRTEQVYIYERFQNQLDSVNYAAHVPQGV
                     KIVAMEGFWRGSSRNYDPKTYVEVDFFGEDDTRSNLARLVCDPLTKIINVPNMKDHGA
                     SGVTGCLKNIAYGSFSNVARSHSDARTHTLSFIGTLAAVEPLRSKTVLQIMDGLRGVW
                     HGGPFSPSRRYRFYPKQMMFGTDPVSIDRLLLDIIENKRKAEGALSVWDRSTAHVKRG
                     GLDSPPDVNRYIREPGHIEYASKLGLGVYDVAKIHVKKIEL"
     gene            18511..19449
                     /locus_tag="VLE22_02300"
     CDS             18511..19449
                     /locus_tag="VLE22_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13259.1"
                     /translation="MIAVILALVLPALYWEQGPETAPTLREAGVECVHVPAASAAAWE
                     KAGFCHVGVSPATLEGFQKALTPGVRMIANEASATRSPWITANGWRFERAKDKRGYLY
                     EAKAGVAALAAAEAFTYDAEALIRIEPTDLKEFGRMLGFLKRVDKPPLPSLADVGVVD
                     DRSALVGEVMNLLLRRNVLFRALGAPDLSYSINIQLGVNYPKSEAANPGAFALKIRRQ
                     LTDDKRLLQIFGSAVVIGRFTSDGKTARLHLLNYGKRPVEGLRVRMRGSYGKPALLVA
                     GQESSTAEDYMVADGGTEFSIPVMGAYSVVDFSVVQ"
     gene            19526..>20121
                     /locus_tag="VLE22_02305"
     CDS             19526..>20121
                     /locus_tag="VLE22_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB13260.1"
                     /translation="MKKILLVEDDSDLFALLKYNLEKEGYAFVGSHTGKGAIELCRRE
                     KPDLIILDIMLPDSDGLEICKGIRSHPELSHIPIIFLTARAAETDRIIGLELGANDYI
                     VKPFFVRELIARVKIQFRVQATPARILKAGPLELDRNSMQVRLYGNLLALTATEFRLL
                     EFLMGRPEVVFNREQLLDAVWGHDRAVTDRTVDVYILRL"
BASE COUNT         3793 a         6212 c         6199 g         3917 t
ORIGIN      
        1 tttctggtct acattccgga tggtataggt gcgagtctcc tggacggcgt ggcgggaagt
       61 aagcacaccc cggcggaaat ggacctcgcg caagatctgg cgcccggagt cgaactgggt
      121 ggtaatgcgt gttccgagat cgacgccata actgatgagc cgcttgtcgg aggccttcag
      181 cgtttcaatg agcgcctcgc cgccgtacgc ggccgaatcg aaaacggtca ccgggccgcc
      241 atcgagtgtc tttccggtat tgtttgtgat ttcggccgcg ttggtcgggt gttgggatct
      301 ctgatcggcg tagatgagca gcttgcgcgc ggcgatcttc tgctgcaaaa acgggagcat
      361 ggccgactcg cctttcctga ccgtcaccgg cgcggaaaag ctgtactcaa acagctcgcc
      421 tagctcacgg ctttcagcgg aggcggcgat ggtactggcg atttcttcac gcggcgccac
      481 ttccgccatt ggcgccgaag ccgcgaggtc gagcatgcgc atttctccgg ctttagcgaa
      541 cgcacgcttg gcgggcgcag gaggcgccgc agcaagttgt tcgacagcgc cgccgatggc
      601 gccttcatgg acaaccgggg cctgcgcgcg ctcctccggc agttcagcgg tcggacggct
      661 gatgtagcgg ggctcataca gcttgctcac gaaggagatc ggccggcctg aaaccagcga
      721 caggcgtacc ttgctccagt cgtagctggt cacgttatcg atgatcgccc atccctcgag
      781 cagcggttca ccggtggctc cgaacaccag ccggtagctg gacttccaca ccggtaccgg
      841 gatgacgtag cttgcgaaga gttgccgggc tgccgagtcg gccgagtcga tgtaaacgct
      901 gcgctgctcg cgcgaacggg cgcccgtcat cgcggccaga taatcgcgaa tctggttctg
      961 gagttcggcg tcgcgaaatc ggacttcggt ggcggtggag agatcgaagg ttcgcagcgc
     1021 acccgagtcg aggagcagcg tgatttgctc gcgctccgga tgctcttggg tcgcggggac
     1081 cagcctcgct ccgacgacgg ctccgctgat cgtctcgggg ccggtgcgaa ggtcgatacg
     1141 cgctcctttc aaggaatcaa tgagagtgct gagcgcctgg cgttcgccga gttgaaaggg
     1201 aaactccgcc agtttgcccg agaggggctc gctcgaatca tagcggacac cggaaatcga
     1261 attacctcgc ttgtcctcga tggtaaggga cttgagaacg tcgttcatct ccgacgcctt
     1321 gaaatccagg cgcgccgcct cgccggccgc gacttctccg gagcgttcga agtaaccgac
     1381 tccgtgtttg tacagaatga cttgcctgac ggggagttcc gcgccttggg caagaagcat
     1441 gatggggagc gagagaagaa cgaagcgtgt catggagcct ccaagggggg gatgccgccg
     1501 gctcactatt cctttcagcc ggcccctttc tatttcagga taatcgcagg ggggcttcac
     1561 aggtgatgac gtgacgcact gcttgacagt gcggaacaag gcgcgtcaga cttttaaggt
     1621 tgccggcgga ggaattgtta cggttcgcgc actccattgt agttgcgtgc gcgtccagcc
     1681 cgcactccaa gacggaggga gtttgcgcac acgaattcta cttgttttga cgaccgctct
     1741 ggccggcgcc gcgttcgcac agagggccga ctatgagggg ccgacgattc tcagccgggg
     1801 tatcgggccg gtattgggag gcggcgggga cctgttgagg atcaggccct atctgagcgt
     1861 tactggggtc tacgatacgg gattaacgcc ggtatcggtg aactcgcaag ggcagatccc
     1921 gaaagaggac ggctacggtg gcgaactcgg tttcggagcc ctggggtacc actcgtggcg
     1981 ggatacggtg cttgggctcg actaccgggg cgacgtccgc cactactcac agcaaagtta
     2041 ctatgacggc agcgatcaca ccctgtcgct tggcgtgacg caccagcttt cgcgcagggt
     2101 ggtgctctcg ctgcgcgagg ccgccggcac gtactcgcga agcttcggcc cgatgggcgg
     2161 gctcggctac ttcgacccgt cgtttgcgga catcccacag aacgaactct tcgacggccg
     2221 cacccactac ctgacgacca tgggtgacct tacgctccag aaagggcctc ggctttcatt
     2281 caatatggga ggtagcggga tggtggtacg tcgccggtca cgttcgctaa taggagtcac
     2341 cggctggacg gcgcgcggcg atacggccta ccgggtcgcc cggaatcaag tcatcggtgt
     2401 ggattacggt tttcaccact tcgactacac cagtgcgttc ggcgcttcgg atatccatac
     2461 ccttggcttg aactggacgg caaggattgg gcggaattgg agtctcggcc tccgggccgg
     2521 tgcatcgagg gtagagacac tcgggctgcg tcgcgtgacg cttgatccga tcatcgcggc
     2581 gatcctcggc cagtcgtcgg gtatcgagac gttctaccgc gtcaactacg tgcctagcgc
     2641 gaatgcacgg ttgacgcgga cgttccggcg ttcgtcggtc agcttcgagt acgaccgtgg
     2701 gacgggttcg ggcaatggaa tatacctgac gtcgcaacag gagagcttcg gtctcaacgc
     2761 cagttacact gcatcccgaa gatggaatgt cggcgcttcc gctggctacg acaatttccg
     2821 cagcctggga cagaccctcg gcaagtacga gtcttaccac ggcggcggcg gtgtgacttt
     2881 tcaaatgacc tcggtgttgc acttcgtggc gcgattcgat ggtcgccact atgacatcga
     2941 aagtaccgac ttcaagcgga cccactaccg ggcgtcgctg ggcatcgcac tgagtcccgg
     3001 cgatatgccg ctctcgctct ggtagaccat gccggaaaca gatcctcgga agcggattct
     3061 cgtggtggat gacgaaccgg cgctgttgaa actgctcgag cggtatctca cacgctccgg
     3121 ctaccgggtt gaggcatgtg gctccgccca acaggcgctc gagcgttacg gagccgaccc
     3181 cggagcttac tctcttgtga tagcggacct gcggatgccc ggcatgtcag gtgaggaact
     3241 gcttgagatg ctcatccggc ggaacccttc gattcggatc gtcgtatgca gtggataccc
     3301 gttcaatcat ctcgagcgac atcccgggat cgaaggccag atccggtttg tgcagaagcc
     3361 gttccttccg agcatgctcg accatgccat tagggaactg ctcggcagtt cttaggagcc
     3421 tgcgcgagga tgttccgata gcgcctcggc aagccgatcg gcgaagttct ctcccgattc
     3481 cgaccagagg cggcggtttt ctgtcggcag cgtgtcatcg agttcatgca cgagccgcag
     3541 tagaccacgg atgttcgatt caactttacg agaactgcca ggggtttcgg gctgtggcag
     3601 gaagtccgcg tcgttgccga actcgatgcg cagatggtcg ccgaggtcgt tctcaaacag
     3661 cgggccataa caggcaaggc ttaccggcgc gggagtgagt ttccattcag ggtcgtatcg
     3721 ccagagatcc cagtagccct caacaatata gccggagtcg gcgttttcga actcccggct
     3781 ggcggcgatc acatcatcca aatcttttgt gagtcggtcg aaaacgcgct cgataagggg
     3841 aggttctgaa aactgttgca cgtaaattcg gagttccacg ggcgcggggg agttgggcga
     3901 aaacggaaac ttccgcagaa gtttctcgaa atgccgtagc atcgtctgcc cgctgtatcc
     3961 gcgaatccag tacgaaagat aaagccggtc agccattgtt cgggtactcc cctccatcca
     4021 ttatgggtcg tcccgaggtc agaaggaccg ggccgcctgc agtgcaatga ccagcccgag
     4081 tagggtaacg atcacggcgc tggtcacagg caatgcccgg accaacggcc cttcgccgct
     4141 caagcgtttc aacgccggtg cagccagcac catggcgact ccgatcgaga tcaacacggc
     4201 ggccagcccg aggctgaaag caaccaggat cgcgagcccg aagccgatcc ggcgcagcga
     4261 gatggagatc atcaacacca caagagcttc ggggcaggga accagaccac cggaaacgcc
     4321 aagcgagagt aaactgcccc gggtcggtgg atcgtggtgg tggttatgcg catggtggtg
     4381 atggacgtga tcgtgatcgt ggctgtgcgt tctcgccgtg agccgttccg aaaggagcca
     4441 cgcgccgatc agcgcaacca gcacgccgct tgcgagtgcc agccaggggt agacccggtc
     4501 gagcgacacc cgttgagacg cgtagagcgt gacaatgccc agcacgaaca cgctcgcggt
     4561 atgggtggcg gtcaccaccg ctccaagaaa aacggcatcg gtgagacgcc cgcggctgcc
     4621 gaccaggtag gcagccacaa tcgatttccc gtgtcccggc gtgagagcat gggccgcgcc
     4681 cagcatgaag gagacgccga gagcagtggc cagaatccag ggatccgcct cgggtgcggc
     4741 taagaacgcc tccaaccgcg aacgctcctg aagcagcagc atgcacaaca gcgcagtcaa
     4801 ccggccaact cctgtaccgc ggcggccgtg cgatcaatct gttcgatcga gttatagata
     4861 tgagggcaga gccggattcc ctcctgattg gaagcggtgg tcgccggtgt catcgcgccg
     4921 gagatgcggt gacgtgacca gagggcatcg taggcgcgtc gcgtgtcggc gtctcggatt
     4981 cggaacttga ccacgccacc ggacagttgc ggctcaagat tcgtcagcaa ctgcactcgc
     5041 ggcatctctc ggagcttctg tttcagagcg ttcgcaagca gacgcatccg cgcctcgacg
     5101 ttcttcatgc cgagaagctg gaggaagtcc gccgcggcat cgaatgcgac cactcgggga
     5161 ttgtcccgct ggccgagtac ctcaaacttc cgggccccca ccagcctatc cgaccaaccg
     5221 gcggtcacga tagagggcca caattcggga atccgttccg agcggacgta cagcacgccc
     5281 gcttcgagtg ggcccatcgc ccacttgtgg gcgctggcgg cgtaggagtc acaaccgatc
     5341 gcccgcacgt tgacgtcgag cgcgccgaag gtctgcgcgc cgtccagatg catccagata
     5401 cctcggctgc gggcgaagtc ggccaactcc ttcacgggat agaggatgcc cgtcgtatta
     5461 gtcacgtgag tgacggcgat gaccttcgtc cgaggagtga cggcacgcac gatcccgtcc
     5521 agaagctgct cacgacccgg cgcggggacg gtaacaggga cgctgcgtac cacgacgcct
     5581 tcgcgtttgg cgcgcatttt ccaggaagct tcgttggagg ggtgattatg gtccgtaatg
     5641 agcacctcgt cgcccgcctt gaggtcgatg cccttaacga cgacattgtt cccctcgctg
     5701 gtgttccggg taatggcaat ctcgtccgcg gaagccccga gcacggccgc caacttggtc
     5761 cgcaattgct cctggagggg ctcgtacttc tcacggttct ggaaggacgg gttggcctca
     5821 aagtcgcgga gaaactcgac atagcggtca aggaccggac gtgaggccgg gcacacgttc
     5881 gccgcatgca ggaagatcag gccgtcttcc agggggaact ggcgctggac catccgccag
     5941 taggcttcgt tggttgcatc ggggatgccg ggcgctgcct cgagagttcg ggtgaacgac
     6001 acccagcttg cgggcagaac cgcggcgccg ggcagagaac gaagaaaacc acgtcgggac
     6061 cgaatcatag gttgactcct cgcattcggt ccctcgattg tacagcaggc acgagtgtga
     6121 agcgtgcctg actcgctatt tcagcttttc tgtaacggtc agcggcccgc tcagacggaa
     6181 gtcgattcgt gtctcgacag ggatgaccgc cggtttgccg cgcgtggcgg cagccgtacc
     6241 ggccccggct gcgccgccga cggcagcgcc gatgcccgca ccctttcctc cgcccgcgat
     6301 tgcaccgatt gctgcaccga tgcctgctcc aataccgacc ttcttcacgt cctctccgcg
     6361 ggacgtgggc gcctgtttgt ggaagaggtc ggtttcgatc gccaactcct gcccgtcgct
     6421 cgtgtgaagc ttcgttaggt gtatagccat cgcggcgagc cccttgactc gcccggactc
     6481 ctggatctcg gccactttgc cctcgagtcg tgcacctcgt tcggcgatca cgagcccgtc
     6541 aaccacaagc ggctggtcca ggttggcggt gaacgtgtct cccagcttca tacggctgct
     6601 gtcgagtgtt tcggcgaggc gtgccgacag gagtgtcccg gcggcgacgg tcaccttccg
     6661 aggctctggc ggaggcgggg gaggggtcac tgccattggc gcaggcgcct tctgctccgc
     6721 cacgttggac gcggccgctg gctgggaaga ctgggccggt gtctgctcag ccttaggctc
     6781 cggggctgtc gtctgggatt tggcggtcac agtcttctgg gccgccggct ttacggacgg
     6841 cgcaggtttc gctctgcttg gcgccggcgc cgcggtttcg gtggctagct gctgtgcttg
     6901 ttcggcaggg ggcggcgccg gtgcctggct ggatggcgcg gccgcctgct cgctggtctc
     6961 agctgtggtt acggctgcct gttcactctg tcgtcctttt aagactaaga acagcaggac
     7021 gccacaggcg ataagggctg ccagtatccc gatctgaacg attttcatgc gcggatctcc
     7081 tccatgatcg tacctattat ccgacggagg cggctcgcat ttcgtttctc cagagccagt
     7141 cgctacaatg gactctagaa tgagggagtt tccgcccaaa ctaagcatcg gcaaggtgga
     7201 gatcgcgcca gcaaccgtcc tggccccgat ggcgggcgtc acggatacgg tgttccgtcg
     7261 tttcatccgc ggtctgggcg gctgcggtct catcatgacg gaatttacca gctcccatgg
     7321 cgtggttgcc tcttccgtcc ggcgtaagcc gacgagaacg ttccactacc tcgtcttcga
     7381 gccggacgaa cacccgatct cggcccagct tttcggcgcc gatcccgacg ttatggcaaa
     7441 cgccgctcgt gtgtgccagg acctcgggtt tgacatcgtg gacatcaacc tcggttgccc
     7501 ggtgaaaaaa gtggtgcgct gcaacggggg ctccggtttg ctgcgagact tgcccctggt
     7561 ggagcgcatc ctgcgggccg tgcgcgccgc aatcaccacc ccgctcacgg tgaaatttcg
     7621 agccggttgg aacgatcggg aactggtgtc cgttccgctt gcgcggatcg ccgaggactg
     7681 cgggctggac gcgattgccc ttcatcctcg tacgcgtgaa caaggataca gcggaaaggc
     7741 ggactgggat cgtatcgccg aagtaaaggc ggctgttcgc attccagtta tcggcaacgg
     7801 ggacgtgatc agtcccgagg acgccttccg catggtccac gaaacaggtt gcgacgccgt
     7861 gatgatcggc cgtgctgcat cctacaaccc gtggatcttc cggcagattg accagtaccg
     7921 cgagaaagga cgctacgatt gcccgaccga gcgcgaccgc tacgacatga tgcgcactta
     7981 ctacgcgcgg ttgatcgatc gcggagagga cgacactgtc ggcaagatga agcagttcgc
     8041 cacctatttc acccatgggg tccgcaacgg ttctcgtctt cgtcaggagg ttcacctcgc
     8101 ccacgccgcc accgagatcg tggaacgagt ggaccgcttc ttcgtccacg aactggaagc
     8161 gtgcccggcc tgacgcgatg aagaaagtcc agatcatcac ggatggagcc tgccttggca
     8221 acccgggacc cggcggctgg gcagcgattc tccgccatgc ccgccacaaa cgcgagctca
     8281 gcggctcgga gccgcacacc accaacaacc gcatggaact gaccgccgcc gttcggggac
     8341 tggcggcatt gaaggaatcc tgtgaggttg aagtgattac cgattccgag tacctgaaaa
     8401 acggcatcac cgaatggctc ccgcgctgga aacgcaacgg ttggaagaca gcatccaagg
     8461 accccgtgaa aaaccaggat ctttgggagc aactcgacgc gctgacagaa cgtcacaaga
     8521 caagatgggt gtggaccaaa ggacacgcga accacgaaga caacaaccgg gcggacgaac
     8581 tggcgtccgc ggcggcacgg ggggtgtcac gatgaggacc ttgcttctct ttgtcctggc
     8641 ggccggcggc gctctcgccc aaccgctgtc cctcggcgtg aaggccggcg tgccgttgaa
     8701 caatgccttc aagatcgcgg acgcctcgac gtatctttcc gaccggcagc cttacgtgat
     8761 cggaccaatg gcggagctgc atctaccgtt ccggctctca gtggagttcg atgcgttgta
     8821 ccgccgcgtg aagtacagtt ccgaaggcgt atcggggtcc gaaccctccg tcccgctcgg
     8881 cacctcgatg acgaccaaag gccgctcctt tgaattccct ctgctgctga agtaccacgt
     8941 tgcggagggc atcgtgcggc cgtacctcgg cgccggactg gtttggcgaa gtatcggagg
     9001 gctgaaacag acgttcctgc cggccggtgg aacaggcctc acagagacgg acgagccggc
     9061 cgaattgcgc gacagttcgg gcggcggcgt tgtcttcagt ggcggcctcg aactccgggt
     9121 cccgttcctt cgcgtttcac ctgagctaag atacacccga tggggtacga cgagcttccg
     9181 gaccgctgtg ggcgacttcc gctcccaggt gaaccaggca gaattcttga tcggcatcac
     9241 cttctaaagc tgcgcggtgc cgcgcgtgcg agacgatcat gatctggaga atgaagcgca
     9301 agcgtcgggc catggaagag gacatcacta tccggtcgcg ggtagcgatc ttcggcggca
     9361 cgttcgaccc gattcacagc gcacacctca tcgtggcaag agaggccctg gaactgtacg
     9421 ggttgtcaca agtgcttttc gttgttgcgg gcaaccctcc ccacaagccg cagaccacgt
     9481 ctgccccgta caagcaccgg atgcggatgg tggaactggc gtgtgaggcg acctcggaat
     9541 ttgtgccatc ccgcctcgag gagactgagg aggtcagtta ctcgattacc acgatcgaga
     9601 aggtcaagaa cctccagggt ccggagggca aactgtactt cctcatcggg gccgatgcct
     9661 tcgcggagat ccgcacctgg tatcgctggg gggacgtgat ccgcggcgtc gagttcatcg
     9721 tagtcactcg tccggggcat cagtataagg tgccgcccgg tgcactggtc cagcggcttg
     9781 aaacgctggc gcttcccgtc tcctcgtcgg agatccgtgc gagactcgcc gccggagacg
     9841 acccgcccga ggtaccccgg cccgtgctcg cctacatacg ggaacacggc ctttatggat
     9901 atcccaggcc cggccgggta tccgccggcc tgtgagggga ttgacgctcc aagcagagcc
     9961 gcaactgaaa ggagcggtga gcgctctcct acacgtccag cccaactgac cgcatcagct
    10021 caagggcgtt gctcttcctt accacgccgg gccgaagaat gtagtcgaaa gtgatccggc
    10081 cgtcctcgat gttgtcctcg aaatgaacat tcgaccccgg aggtgacagc acctcagcga
    10141 tatgcgccag ggcgaggtcg tgggtcgtca ccagcccgat cgcgccgcgt tccaccaggc
    10201 cgcgaaccac ggcctcggat ccggttcgcc ggtcgtgcga gttggttccg tggagcaact
    10261 cgtcaagcag gaagagcacg ggccgctggc cgccgcagag gtcaaccagg cggcgtaacc
    10321 gtttgatctc cgcgtaaaac cgcgacgtcc cgccctggag cgagtcaact acgcggatgg
    10381 aagcgccgac taatagttcg gaaagccgaa gccgtgtcgc ccgcaccggg gcgcctgcca
    10441 tcgccagcac ggcgttgacc ccaaccgtgc gcagcagtgt gctcttgccg gacatgttcg
    10501 atccgctgac gaccagcatc cggatgtcgc ttccgaggcg cagatcgttg cgaacgcaac
    10561 gagcttctgg gatgagcggg tgtccgagac cttccccatc gaagcgcggt ccgtcttcgg
    10621 tgagttccgg gaacggatca gccgggtgtt cccacgagta gcccgacagc gacgacaggg
    10681 cctcgatctc tcccaccgct tcgagccagc ggcgtacggc cgggccgtgg tgcttgcgcc
    10741 agttttcgac tgcgaaggcc agttgtgtcg tccacaggag cggcgggccg aagacccgaa
    10801 gcaccacgtt ctcccgggaa tcaagcaatt ccatgagccg gttgagccgg gcgatcgacc
    10861 gcgacggcga ctgcgtttcc gtatccagcc gcgcgcgtag ttccgccagc attgggctgt
    10921 ggaacttctc ccgctccaac cgggccagca cctctgagag cagggcaagg tcgtgagagg
    10981 gaagttccgc ggcatgaact gctcgttcca ctcgtttccg gtaatgcaag ccgaatgccg
    11041 cctccaggac cagcaccgca aaaaaggcga tgcgatggcc ggtctcgatc cagaggacca
    11101 gcgcggccac tgcagcgagc gcaagagtgg cggcggcgat ccgcgcccgt ggagagtcga
    11161 gaatcggcgg ctggtctccc catgcggcca gcggctccga gtggagcccg gcccggacct
    11221 cctcgccgag caccgcaagg tcctcccgaa ggtcaagccg cgtccgaagc tcgtccacgg
    11281 cggcgtgacg cgcatgcaag gcgtccaatg gagcaggcga aagcagccac ctcgcgagtg
    11341 tttcctcccc ggccctcgtt cgcgcaatcg aaagcagttc gaacaggcct cccttgccga
    11401 agagatcgag gtcatctgaa tagaggtgct ccttgctgga gaatcggtca ccggcttcgc
    11461 ctgtccccgc ccaacggtca cttaaccgcg cgagaccctg atcgtagaat gcggcggccc
    11521 ggagttcgtg gctgcgcgcc cggaggaccc ggtcgtgcag gatggcgagg acgacgaagc
    11581 acgccgccgg cgccagcagc caccagggcg tgagggtgtc acgaaccagc gcgagccagc
    11641 cgattagggc ggccatcacc gccactgcca ggcggaggtt gccgaatcgg acgtggagac
    11701 gctcgtgccg cccagcgact gccagcttgg cgtccctgcg acggatgtac tcggctttgg
    11761 gatcggaccg gctggagccg ggtgcatcag tcatgtgcga aaccttgaac aagtccctag
    11821 ggttcttccg ccactttccg caggactttc agacactcga ttaatgctcg tccgttgtgg
    11881 tagccggcct tccatagatg acctttgtcg gcgtccgccg gtccatccgg cgtgacaatg
    11941 gcgtgccatt cgccactctt ccagtcgatc tggtgctgat ccacgaactc ccacgttttc
    12001 tcgaatacct cgaagtactt ggggtctccg gtgaagtcga acatccgcag tgccgcgaca
    12061 agggcctcgg cctgcaccca ccagatcttg tcgcgccggt cggcgagtag tcccgggagg
    12121 ccgctctcgt aaaaccctcc gcgatactcg tcgttgccga acttcagagc atgggcgaac
    12181 agagtccgat agaggtcgtg gaggggatgg tccggaatcc ccgccgcccg gttggcttcc
    12241 atgagcagcc agatgttctc gacgtcgtgg cccggcgaca cccgcgcgtt gtcgccgtcc
    12301 atcaatggct cccattgccg ggtgtacttg tccgtgcatg caccggcgtc cttccgcacc
    12361 acggtattgc tttcgattgt gatcagctcg agcagccgct cacgcgcctt ttcaagttcg
    12421 gacgcctccg cgaagaccgt catcgcctcc atgaggtgca gatgcgagtt catcgtcttg
    12481 agacccgccg gccgcatata agtggtctcc ccgtccggga ggggtgtcca gtcttcctcg
    12541 aacgcctcga tgtaacctcc gtatgttctg tcgtgcgcct tagcgtccag cagacggaag
    12601 aactgttccg cgaaatcgag tacttctttt cgccggcacg caaggtagta ctccgagaga
    12661 gcgtaaagcg caaacgcctg gccgtaaatc atcttgcgcg ggcggagctg acggtctccg
    12721 gtcgcatcca cctcccagta aaagcccccg tgctcgtggt cccacatctt ttcacggaga
    12781 aagcgataac cccaatctgc cgcttccagc atctcgcggt tgccccagcc cggtcctttg
    12841 aatccacccc gagccagccg cgcgaacagc cacagtgtcc ttgcttgcgt gaccagaccc
    12901 ttcgtatatt cccgcttcgg ctcgcccttc gggtcgaacg cgatgatgta cccaccgtgc
    12961 ttgcggtcca gcgagcgtgg ataccaaaat acggcgatgt tctctgtgag ggcgcgttcc
    13021 aggcgcggaa tcagggcccg ggcccgcttt ggaattgacg gagcaggtcc ggacgcctgt
    13081 gcagcgaaca gcagcaggag cgcaaccgcg ggcgcggtca tggccttgag cgtcgtgcgt
    13141 cgatgaacga cttcacgcac agaccgacga agaaagcggt gaagagcgcc ataatcgact
    13201 gcgcgagcac cgccgccggc cgttcgagcg gctcgccctg caacagcgcc ggcagtttca
    13261 tcagcccgcg taccgaaccg agaaagccga gcagcccgac cgcggccgcg atatgcatcg
    13321 ccatcttgcg cttcttgtcg tcgcgggcga gaatccccag cgccagcaga acgatgccga
    13381 agaacgccgg aatcagcgcg gtcacgctca caaagcccga gaatacgtag cccgccactc
    13441 ccagcaggat cagtgccacc gcgatgccaa tcgtttttgt cgccatgttt ctcctttcga
    13501 cgaaccgtca ggccttgggt gtgagcgttc gctcggcgcc aaggccgctg atcgcgtcgc
    13561 ggactcgaat ccgccacgca ccccgagcgt cgctcatcgc gaacgggatc tcgatgcgcc
    13621 cggcgccatt cacgatatcc acattcatgc agtattcgcg gaccaggcga tcttccggat
    13681 tgaagacctc gacatgcacc accgagcggt ctaccggagc gcctgcggcg tcgagcagcc
    13741 tcaagttcaa attcgcgtcc atatcgagac gcgtgatggc tgactgccgc gcaagaagca
    13801 aaaccggtgt accgggcgtg gcttccaacg tgccgcgcgc cgtctcaccc cggatgaggt
    13861 cgtaaatgac gccctccatt ggcggtagtt tgtagattgc cttctccttc ggcagaagtc
    13921 cgatcagccg ggaaccgccg aagtcgcgaa cggtgacaag gaactttcca tccgtcggta
    13981 cagccgactc caactcgatg tcgcccggcc cccttcgtat gagacccttc cggccgtgtt
    14041 gccagagcac tgctttgcgc caccggcagc gctcgtccat cttgcctgtc tgctcgtcgg
    14101 cgtagaccac gccgccgcgg tccatgaagc gttcgatctc ttggatctca cgatcggaga
    14161 gagcgatcga gtacggcagg atcaacaccc gatagttctt cagatcacca gcctcgatct
    14221 gcggcgtcgc caggaagcgg agttggagtc cctggcgctc cagatcctcc acccacgcgt
    14281 tgcgcagttt gttgagctca gcgaagttct ttgaactcct cataacgttg cccatgtcgg
    14341 cgctgagttt tccgtcggtg atccaggcgc cccggatgct tgccatcgag aagtgaaccg
    14401 ccacgccgtc ctcgcggacc ttcgaattca tgaagatccg gccgatgccg gactgaaggc
    14461 gcccaaacgc atccgccaac gcggctccct gctcgctcag cgtcaggtcg ggattcatca
    14521 gtgtgtagta ccagaagatc gaggcaccgg tgtggccgta aaacagccgc tgccactgct
    14581 ggtgcttggc ctcgtcgccg acgagcccgt agcccgtgaa gccggtgatc tgaagcttcg
    14641 gattgaacag gtaatgcatc gcatcctggc ttccccccga ataaggctgg atgtattcaa
    14701 gctgctggtc gatctcgtac cagttacacc cgttgtgggc cgtcggaatc tgcgtacccg
    14761 aaatggacgg ccggccctcc gggtcgatct cacgcaacca ctcgcgtgct ttggcgaagg
    14821 cttggacgaa ttcctgctcc atgtagacac ggtggtccga ccacggtgcg aagttgccgt
    14881 gtttctgcgc ctcctcggtc gtcatcggca ccacggaatc ccattcctga aacgacgtcc
    14941 cccaggcgct gttcaatttc tccaggctgc cgtactcttt tcgaagccac tggcgcaagc
    15001 cggcgatcgc ctcgggcgcc caatccacgt cgaacgggtc ggtgtaacag gtcagcgacg
    15061 actcgtcggc gaggtagtag gcccacggct tcagcgcggc gaccgggcgg acgttctttt
    15121 cgagggatgc cttcacgctc ttctcgaatt cgggcgattg aagcacgacc tcacgggtca
    15181 gatacttctt atccccggtc tcggcgaacg ccttcttccg cttgaggtat ccgtcccggc
    15241 ggtaccagta aatgccgaac gcgagtgggt gaacactcgc catgatcttg aaattgcgct
    15301 ccggtctggc tagtgtcgtg ataccgatgc tccggagttg ctcgtctaca gtcgggatcc
    15361 acggctggta gctgcgcgga ccgaaccacg gcaagaggat ttcgtagtcc ttccactccc
    15421 gagaagacgc ggcgaagcgc accggaatct gagcgacggc actcccggcc gtggcccgca
    15481 cccagcccga gtggaccaac ggcttacccg ccttgagtcg cgctttgtcc tctccggaag
    15541 aacgcgcaat cacacggccg aggccatcga tcagttcaat cctcgcacga cgcgtagact
    15601 tccaggtgac atcgacggca tcgccttccg aaatgatctc cttcgatgct ttcagctctt
    15661 caacccggtc ggactgctgt acgtcgagaa cggtgatccg ccactccgcg ccattttgct
    15721 gttcgaggaa ataacggccg ccttcgagcg tctgagattg cttggtggga ccttgtccgg
    15781 aatcgcggat tctcgaacgg agatctctca cgcgccactc tgtcgaaggg acctcccagt
    15841 ccggtatggc ggcgggttcg cgcttcgcta cgtagatcag cgcacggcac aacagcgaat
    15901 agaaatattc ccaatacgta tcgacgaaat cattgcgcgc ggtttgaggc atgtaccagc
    15961 tcacgccggc attccgatac ccgaaggcgg ccactcttcc gcttccgtac gtgtgggcag
    16021 ccagtatggc atggcccgtt tcggactcga tcaggacctc cgctcccgcg gcggcgcggc
    16081 agatgtactg gtcgagatgc tggaacggaa agctctccac cggaactgcc ctggtgatgt
    16141 agtgttcacc cttccggtgc cacggcgagc tctccgtctt gcccggttcg cggggcacct
    16201 ccagttcgtc ttcggactcc ggcggcgctg tagtgggctc caacgggcag atttcgctgg
    16261 aaaacggacg gatgagcacg agaccggacc cttcctttac ccttcgggcc agcgcctcgc
    16321 gcgacgcgga agtcagcgcc ttccaaccgt gactcagcgg aagcaggatc gcatcgaatt
    16381 gcttcgttga ggtcagttcc tgttccaggt acgagtagac gagattcagg tctccccgct
    16441 ccgcgcgggc gccgtagtca cggccgaagc acatcgtcca cttgttgacg tcccaggcag
    16501 ggtcgatgct cacggtggtg agatcgaggg acaggcgctg cgccagttcg acgacagttc
    16561 ggccctcatc aaccgaggac accgcgagca ggcggatcgg gccgccgagc agtggctttg
    16621 cccacttgac gtgcggcgtc tcgatctcga gcgtgtaagg ggtctccaga cgtttggagc
    16681 tccccttgcg cgcgcccatg ccgacgtcct gcgcgtgcgc cggtgtgcgc gccgctgtgg
    16741 cggctgtagc cgccgcgacg ctcgtcccga ggaattgtct tcggttccag tcttgcttcc
    16801 tcattacttc atctccaggt tcaattcgct ccagtagaca gtcgacggcc tgcgcggggc
    16861 ggagcgatgc gccgcccccg gcacattcgg aagaaagtct cagatgtacg cgatgacgtc
    16921 aatctccacc agcgagtctc cggggatccc gccggctgct gcaatcgtcg tgcgcactgg
    16981 tggttcactg ccaaaacggc cgaggaaggc ctcgttcatc gccttgtaat ccttcaaatc
    17041 gttcaggtac acgttgcact tcaggacttt atccatcgac gagccggcgt tctccaactc
    17101 cttctggacc tcgtccagca cgtgttttgt gtgcgctgtg atgtcccctt ggaagtgtgc
    17161 gcccttcccg gcgatgaaca gcagatttcc gtaggacacg gctccggaga acagcggagt
    17221 cttcttcggt ttctctccgc gatagtgaac cttcttaacg ggtccacctg cggccttggc
    17281 cgcacccgcg gcggctgcga cgccggcggc cacttttagc aagcttctgc gattctgttt
    17341 catgaattcc tccgtgacgt tcaaaaggtg taatcataat tctatgagac gacgcgcctt
    17401 tctgggaatt ccggctgctt ctgcattctc gttttccgcg gattcggcgc cggtgcccag
    17461 atacaaggtg gtcagcgcct ataaatcaca ggccggccag ggcatgcccg gcttgtttcc
    17521 cggatccgtc gtttcggtgc attcgaatac atccatcgac gaaaccaccg agaaggtcaa
    17581 cgcgccagcg gtggcccaga tggtggccga aggaatgaag acgctatgcg gagtcaagga
    17641 cccgcgagac gcctggcggc gcttcttcac cccgcaggac gtcgtcggaa tcaaagtcaa
    17701 ctgttcgggc gcccccggaa tcatgtcaac gccggcagta gtggcggaga tcgtgcgaaa
    17761 tctggtcgcg gttggcgtca ggacagagca ggtctacatc tatgagcgct tccagaatca
    17821 gttggatagc gtgaattacg cggcgcacgt gccccaagga gtgaagatcg ttgcgatgga
    17881 gggattttgg cggggcagta gccgcaacta tgatccaaag acctacgtcg aagtggactt
    17941 cttcggcgaa gacgacacgc ggtctaacct tgcccggctc gtttgcgacc ctcttacgaa
    18001 gatcatcaac gtgccgaaca tgaaggacca cggcgcctcg ggcgtgacgg gctgcttgaa
    18061 gaatatcgcc tacggcagct tctcgaacgt ggcgcggtcg cacagcgacg cgcggacaca
    18121 cacgctttcc tttatcggga cgctggccgc ggtggagccg cttcgttcga agaccgtgct
    18181 tcaaatcatg gacgggcttc gcggcgtctg gcatggaggg cctttctcgc cctcacgccg
    18241 ttacaggttc tacccgaagc aaatgatgtt cggcaccgac ccagtgtcga tcgaccggct
    18301 gttgctcgat atcatcgaaa acaagcggaa ggcggagggt gccctctctg tctgggatcg
    18361 ctcgacggct cacgtgaaac ggggcggtct cgactctccg cccgacgtga atcgctacat
    18421 acgtgaaccc gggcacatcg aatatgcgtc gaagcttggt ctcggagtgt atgacgtcgc
    18481 caagatccac gtgaagaaga tcgaactctg atgattgccg tcattcttgc ccttgtgttg
    18541 ccggcactct attgggagca agggccggag accgctccca cccttcggga agccggtgtg
    18601 gaatgcgtcc atgtacccgc ggccagcgcc gctgcttggg agaaggccgg attttgccac
    18661 gtgggtgtca gcccggctac gctcgagggc ttccagaagg cgcttactcc gggtgtgcga
    18721 atgatcgcca atgaagcctc ggcgacgcgc tcgccgtgga tcaccgcaaa cggctggcgt
    18781 ttcgagcgcg cgaaggataa gcgaggctac ctttatgagg cgaaagccgg tgtggctgca
    18841 ctagcggcgg cagaggcgtt cacatacgac gcggaggccc taatccgcat cgaaccgacc
    18901 gacctcaagg aattcggacg tatgctgggg tttctgaaac gggtggacaa accgccttta
    18961 ccttctctgg ctgacgtcgg cgtcgtggac gaccgctcag cgcttgtggg cgaggtcatg
    19021 aacctattgc tccgtcggaa cgtgctattt cgtgcgctag gagctccgga tctgagttac
    19081 tcgatcaaca tccagttggg cgtgaactac ccgaagtccg aggccgccaa cccgggagcc
    19141 ttcgctctca agatccgccg gcaattgacc gacgacaaac gcctgctcca gatcttcggg
    19201 agcgcagtcg tcatcggacg gttcacttcg gacgggaaga ccgcacgcct ccacctgctc
    19261 aactacggta aacggccggt cgaagggttg cgcgtgagga tgagagggtc ctacggcaaa
    19321 ccggctctac tcgttgctgg tcaggagagc tccacggcgg aggactacat ggtcgcggac
    19381 ggcggcacgg agttcagcat cccggtgatg ggcgcctact ctgtggttga tttttctgtc
    19441 gttcaatagc aatgcaacag caacgtcact ggattgtcac gagttcgtaa caatcgttgc
    19501 gctatcttta cggtaagcaa gacttatgaa aaagattctg ttggtcgaag atgacagcga
    19561 cttattcgct ttgttgaagt acaacctgga aaaagaaggc tatgcattcg tcggatcgca
    19621 caccggcaag ggcgccatcg agttgtgccg ccgcgaaaag ccggatctca tcattcttga
    19681 catcatgctg cccgactctg acggtctgga gatttgcaag ggtatacgga gtcaccccga
    19741 gctctcccat attccgatca tcttcctcac ggcacgggcg gccgaaacgg accgcatcat
    19801 cggcctggag ctcggtgcta acgactacat cgtgaagccg ttcttcgtcc gggagctgat
    19861 cgcccgggtc aagatccagt ttcgcgttca ggccacaccg gcaagaattc tgaaggccgg
    19921 tccgctcgaa ctggatcgca acagcatgca ggtccgactc tatggtaacc tgcttgccct
    19981 gaccgcgact gagttccgcc tgctcgagtt tttgatgggc cgccccgaag tcgttttcaa
    20041 ccgcgagcag ttgctcgacg cggtgtgggg gcacgataga gcggtcacgg atcggaccgt
    20101 tgacgtctac atcctccgtc t
//
LOCUS       DATIFQ010000051         5753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_781361, whole genome shotgun sequence.
ACCESSION   DATIFQ010000051 DATIFQ010000000
VERSION     DATIFQ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781361"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VLE22_02310"
     CDS             complement(<1..189)
                     /locus_tag="VLE22_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13261.1"
                     /translation="MNRTSMSNVALSLLMLALLAPTAWPQASTATVSGTVRDQSGAVV
                     PTASVTLTNSATNIVSKTT"
     gene            547..2787
                     /locus_tag="VLE22_02315"
     CDS             547..2787
                     /locus_tag="VLE22_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB13262.1"
                     /translation="MAAVFKLYRFDDAFQSSAAVSGSLAPSSALMADPVIWHNFFSPK
                     DIPWILLRGQIGYDDHGDLLLKGQGATPVILAPKRPLVEWNRYEAVEIRMLAEGGKEI
                     KIRIGDREYKQKLGPLHQYNDYRFDIRIDESGMRPLALMPTDSLVDKVAIKSIRLVPR
                     KASFPQIAGRKFIGKREEYRNTLYVHSPSTVTFETPVPRNGRLHFGMGVVERNRPVTF
                     RVVAGSKELYSKTVSDPDSWDDAEVDLTPYGGGSVKLAFETSVEGQGAVALWTNPLLT
                     SKAPKNRPNVLIYMIDTLRADHTSLYGYARDTTPFLKELGTQGLAFEDCQVQATWTKP
                     STASLMTSLYSYTHGIIRDYDTIPKGATTLAEQLRAAGYVTAGVVANPFAGLLSGLDR
                     GFDYMSEWLAVQRYRNEAEDRSTDSAAVNKILFPWLERHRDEPFFIYAHTMDPHAPYR
                     PPPGFEEKFANPAETPEFDRTCRKLEGTARYGGGIVVSRAGCARRGVNPERFIQRAID
                     RYDGEILHNDRSLEQLVGKLRQLGILDNTLIVVVSDHGEEFWEHGWTAHGHSLYEELT
                     NGVFLIWNPKLIPTPRKIKEPVQLIDVLPTVLDLLGLKIPDVVQGQSLAPFVKGQAFK
                     RRGPVMTSRFAHPEAKRTAAIPENRIDTVALLDTNWKLIYREDAKAAGMNAVELYDRR
                     SDRVEARNVAEQHPREVDRMMAEISTWMEAQKRVRSLLGRGAHVTPDPRTIEKLRSLG
                     YLGGKQ"
     gene            complement(2932..4350)
                     /locus_tag="VLE22_02320"
     CDS             complement(2932..4350)
                     /locus_tag="VLE22_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB13263.1"
                     /translation="MIVTDDQGYADLSAYAHSAADVSTPNMDRIAAGGVLFTRAYVTA
                     PVCSPSRAGWNTGRYQQRWDPDAGWNPGLPSDSKTIAEYFKSAGYATAKVGKSDFGRG
                     YHRQDGREYPLNHGYDEFLGFSSHAHDYFLLSDKSEKMTPDPYGHSAALGTLFENRGR
                     KSFESGYITEIFTDRAIQFLERNRSRPFFLNVAYNSVHTLVHEVPEKYLEKFGVKPIP
                     NYDPRTMGKYVDYYDKYAQLGIISGEEMRKYYLANLNCLDDNIGRLLNALDRLKLTEN
                     TLIVFFSDNGGTPHGGGGNLPLRGSKSTTFEGGIRVPFMMRWPGRLPAGGTYAYPVST
                     LDLLPTFLDAARIPALPSATLDGESFLQAVKAGRPSRNESRPLFWLFRGRWAVLSGDW
                     KLVKTSGPGGPPAHQILSGGDPAEPKPALFNLKSDPAEQHDVSGQHPDVVKRLNRLFE
                     DWRQQTRTEALSGRGSSAPPKR"
     gene            5555..5752
                     /locus_tag="VLE22_02325"
     CDS             5555..5752
                     /locus_tag="VLE22_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13264.1"
                     /translation="MRRRAPRNRHADLVFNNINPTNGIVEIRFTGNELRGTQCEAVIE
                     ALEIGAGYGGSGDTPKTVVGL"
BASE COUNT         1215 a         1648 c         1752 g         1138 t
ORIGIN      
        1 cgtcgtcttg gaaacgatgt tcgtagcgga gttcgtgagc gttaccgatg ccgtcgggac
       61 cacggcgccg gattgatcgc gcaccgttcc gctgactgtc gcggtgctcg cctgcggcca
      121 cgccgtaggc gccagcaacg caagcatgag aagggacaaa gcgacattgg acatagatgt
      181 ccgattcata ttgcctcctc ttcctgactt ggggcctatt atgtgccaga ttgccggcgc
      241 tgtcaatcgc taagctcatt gcgctaagtc cattggggcg gcgccacact cggcgacggc
      301 gggccttccc gcggcgtacg tggaaaatga cataccgctg cgcagcggca ttaacaatca
      361 ttaaagctaa gtctcaaact ggtgttcgca atcggtcccc ggattgctac cgtgaccacc
      421 tgagcgagta accactcccg cggagtgtgc aagaatggca ccgtgcgagc gcctcttctc
      481 gcgggatgca tcggggtact aatcctggcc ccgggctgcc gaaaggccgc tgctcctccc
      541 gagtccatgg cagccgtatt caagctctac cgtttcgacg atgcgttcca atcgtccgcc
      601 gcggtttcgg ggtccctggc gccctcctcg gccctcatgg ccgatccggt catttggcac
      661 aacttcttct cgccaaagga cattccctgg attctactcc gtggccaaat cggatatgac
      721 gaccacggcg atctcctgtt gaagggccag ggcgccacgc cggtcatact ggccccaaag
      781 cggcccctcg tcgagtggaa tcgttacgag gccgtcgaaa ttcggatgct cgctgagggc
      841 ggcaaggaga tcaaaatcag gatcggcgat cgggaataca agcagaaact gggcccgctg
      901 catcagtaca acgactatcg cttcgatata cgcatcgatg aatccggcat gcggccattg
      961 gctctcatgc ccacagacag cctggtcgat aaggtggcga tcaagtcgat tcgcctggtg
     1021 ccgagaaagg cgagtttccc acagattgcg ggccgcaagt ttatcggaaa acgggaagaa
     1081 taccgcaaca cgctctacgt gcactcgccc tcgactgtga cattcgagac accggtcccg
     1141 agaaacgggc gattgcattt cggcatgggc gtcgtcgaga gaaaccgtcc cgtcacgttc
     1201 cgggtggtgg ctgggtcgaa ggagttgtat tcgaaaacgg ttagcgatcc ggattcgtgg
     1261 gacgatgccg aggtggatct taccccatac ggcggtggca gcgtcaaact tgcgttcgaa
     1321 accagcgtgg agggccaggg cgccgtggcg ctgtggacca atccgctgct caccagcaaa
     1381 gcaccgaaga accgtccaaa cgttctcatc tacatgatcg atacgctgcg ggccgatcac
     1441 acaagtcttt acggctacgc ccgcgacact acgcctttcc tgaaagaact ggggacgcag
     1501 ggcctggcat tcgaagattg ccaggtgcag gcgacatgga cgaagccgtc caccgcgtcg
     1561 ctgatgacct cgctgtactc ctacacccat gggatcattc gggattacga cacaattccg
     1621 aaaggtgcga ccacgctggc cgagcagttg cgggcggcgg gatacgtgac ggcgggcgtc
     1681 gtggccaacc cgttcgccgg cctgctctcg ggcttggatc gcgggttcga ttacatgtcg
     1741 gagtggctcg cggtgcagag gtacaggaat gaggcggaag accgcagcac cgactcggcg
     1801 gctgtgaaca agatcctgtt tccgtggttg gagcgtcaca gggacgagcc gttcttcatt
     1861 tacgcgcaca ccatggatcc acatgcgccc taccggcctc cgcccggctt cgaagagaag
     1921 ttcgccaacc cggcggagac accggaattc gatcgtactt gcaggaaact cgaaggtaca
     1981 gccagatacg gaggaggtat tgtggtcagc cgcgcgggtt gcgcgcggcg cggagtgaac
     2041 cccgaacgct ttatccagag agccatcgac cggtatgacg gtgagattct gcacaacgac
     2101 cggagtcttg agcagctcgt gggcaagctg aggcaactcg ggattctgga caacacgttg
     2161 atcgtggtgg tttccgacca tggagaggaa ttttgggaac acggatggac cgcgcacggc
     2221 cactcgctct acgaggaact gaccaacggc gtgttcctga tttggaaccc gaagctgatc
     2281 cccacaccgc ggaaaatcaa ggaaccggtg caattgatcg acgtgctgcc gacggtactc
     2341 gatctgctcg gcctcaagat accggacgtc gtgcaaggac aaagcctcgc cccatttgtc
     2401 aaagggcaag catttaagag gcggggcccg gtgatgactt cgcggttcgc gcatccggag
     2461 gcgaaacgta ccgcagccat tcctgagaac cgcatcgata cggtggcgct cctggatacg
     2521 aattggaagc ttatctatcg cgaagacgcc aaggcagccg gaatgaacgc ggtcgagctg
     2581 tacgaccggc gcagcgaccg cgtggaagcc aggaacgtgg cagagcagca tccgcgcgaa
     2641 gtagaccgga tgatggctga gatcagtaca tggatggagg cccaaaagcg ggtccgaagc
     2701 ctcctcggcc gcggcgcgca cgtgacacca gatccgcgga ctatcgagaa actgcggagc
     2761 ctggggtatc ttggtgggaa gcaatgattt cttgccgcta ttcctgagcc cacacgcgga
     2821 tcggcgtgcc gtcgaatgtg tccgagccga acgccgtcgt aacgcagtct gccgccacag
     2881 catacggtcc cgcgtgattg caaatccagt agccgttgca gaggaataaa gtcagcgttt
     2941 cggcggcgcg gatgatcctc ttccggacaa cgcttcggtc cgcgtttgct gccgccaatc
     3001 ctcaaacagc cggttcaagc gcttgacaac atcgggatgc tgcccggaaa catcgtgctg
     3061 ctcggccggg tcgctcttga ggttgaacag agcgggcttc ggctccgcag gatcgccgcc
     3121 ggaaagaatc tggtgcgcag gcgggccgcc tggcccggaa gttttgacga gcttccagtc
     3181 tccgctcaga acggcccagc gacctctgaa tagccaaaag agcggccggc tttcgtttcg
     3241 ggaaggtctg cccgctttta ccgcctgcaa gaagctctct ccatccagcg ttgcagaggg
     3301 aagcgctgga atccttgccg catcgagaaa ggtgggcaag aggtccagcg tcgaaacggg
     3361 atatgcgtac gtaccgcccg cgggaaggcg gcctggccac cgcatcataa acggcacacg
     3421 gatacctcct tcgaaggtgg tggacttgga tccgcgcagc ggtagattgc ctcccccgcc
     3481 atgcggtgtg ccgccgttat cggaaaagaa gacgatcaat gtgttttccg tgagcttcag
     3541 gcggtccaat gcgtttagca atcggccgat gttgtcgtcc aggcagttca ggttcgccag
     3601 gtagtatttg cgcatctctt cgccggagat gatgccgagc tgagcgtact tgtcgtagta
     3661 gtcgacgtac ttgcccatcg ttcgggggtc atagttcggg atcggcttca caccgaattt
     3721 ctccaagtac ttttcgggaa cctcgtgaac cagtgtatgg accgagttgt aggccacgtt
     3781 gaggaagaac ggccggctac gatttctctc gaggaattga atcgcccgat ccgtgaagat
     3841 ttccgtgatg tagccgcttt caaagctttt gcggccccgg ttttcaaaca gcgtgcccaa
     3901 cgcggcgcta tggccataag gatccggcgt catcttctcg ctcttgtccg aaagaagaaa
     3961 gtaatcatga gcgtgcgatg agaagccgag gaactcgtcg tagccgtgat tgagagggta
     4021 ttcgcgtccg tcctgcctgt ggtagccgcg gccgaagtca ctctttccga ctttggcggt
     4081 tgcatatccg gcactcttga agtattcggc gatggtcttg ctgtcagacg gcagtcccgg
     4141 gttccagccc gcatccggat cccatcgctg ctgatagcgc cccgtattcc agccagcgcg
     4201 cgaagggctg caaaccgggg cagtcacgta cgcccgcgtg aacagcacgc cgcccgccgc
     4261 aatccgatcc atgttcgggg tggagacgtc cgcggctgag tgcgcgtagg cgctcaagtc
     4321 cgcgtatccc tgatcgtcag tgacaatcac caagacgttg ggcctggcga cgggttgcgc
     4381 accacgctcg gaactcaggc caccgaatgc ggcgccgcag atctggcgga gaaaatcggc
     4441 tctggttaat tcaaagttgt tcgtgtgggt ttcccttggg cgggattatc gcacattcgt
     4501 gatttgtacc tatcgcaaat ctcaagggga gttgggtgca cgcgcttgac catcctcgaa
     4561 gaatcaacaa gttagacaga tcgcttccaa ccgctactga gtacttttct caatcacttg
     4621 gccgtgatgg aaggccacat ggtagcagtc catttatgca ggtggtgggc gtacctgagg
     4681 gcataccgtc gtacgctcgg cagcatcgtt tcgttcattc gctgcaacca atgcggcggg
     4741 aacgctttag agcgttccgt gcccacgcag ggaactagcg gtatgctctc cactcggatg
     4801 gtcgaatcgc cttccgccga tccaagaact tcaaacagcc ttttgcgacg ggccgcgcgg
     4861 cgctccgctg ctgtggggcc tccgttcttt ggctgccagg cgaacatggc ttcggtatcc
     4921 tcctacctgc tattcttgcc gccgttcaac gcgttgcata tagaacctcg ccgacgaatg
     4981 gctccagaac gtcgatatct ttgggtccag tacctccccg aacagtgaga acgtagcagc
     5041 aaaaagctcg gtagcgacct ttggcaagct cgagtgcgtc ggctgcttgt gcttgtgcct
     5101 gagttggcac accacgacga agaaaccagt gctgcgagtg gaactccagg ccgaaactct
     5161 atctgacgcg gcgactgttt ggagggatgg tgcggtggaa tgcggaattg tcagtgccga
     5221 cggaatagcc ggacgccgcg tcagcaggaa catcggtcgt cgaaacggtg ggggaaggaa
     5281 aggtgttccc tggaatcagt tggaaaagcg acagttccct gacttcgtcg tcctccagag
     5341 gggaccgacc tgatggagtt tatcgcccgg acggggaatt taaccgatgt ggtcgcgaaa
     5401 acgtggtcga cgatgcggca ggctgtcttc atcggcgacc cgaacgagca gaaagaccag
     5461 gaactgtacc gctatgagac ccaacatgac cgagcgcgcc aaagagccat gaccatatcg
     5521 attaacggcc gcaagatgga gggtttcgac gtctttgcga cggcgggcgc caagaaacag
     5581 acacgcggac cttgtcttca acaacatcaa cccgacaaac gggatcgtgg agatccgatt
     5641 cacgggcaac gagttgaggg gaacccaatg cgaagccgtg attgaggcgc tcgagatcgg
     5701 agcgggctac ggcggatcgg gcgatacgcc gaaaaccgtc gtcgggcttt gag
//
LOCUS       DATIFQ010000052         4420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_16459, whole genome shotgun sequence.
ACCESSION   DATIFQ010000052 DATIFQ010000000
VERSION     DATIFQ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16459"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VLE22_02330"
     CDS             complement(<1..197)
                     /locus_tag="VLE22_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13265.1"
                     /translation="MVPASLAPRKPDFENFRRTILRQGEPSHIPIVEFGVHQDVKAAF
                     LGRPVRTLRDEVEFWVRAGYD"
     gene            562..1215
                     /locus_tag="VLE22_02335"
     CDS             562..1215
                     /locus_tag="VLE22_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB13266.1"
                     /translation="MDGSKVYVDSSVVLRKLLDAPGAVPHWGSWTLAVASELVQVEAL
                     RTLDRLRILGELSVTEYADCVEELGLWLSAFELAPIDRAVLRRAAGPFSTAIGTLDAI
                     HLATALLWMEREPAELTLVTHDRQQAAAARACGIGGSVQPVAGLLDAPCGQGLNRLFP
                     FSREAAGKPGCESGLCLGRARDQARRGLERRAEFRLKPRISSQNVTPVDEPLDQDGP"
     gene            complement(2551..4014)
                     /locus_tag="VLE22_02340"
                     /pseudo
     CDS             complement(2551..4014)
                     /locus_tag="VLE22_02340"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
     gene            4095..>4420
                     /locus_tag="VLE22_02345"
     CDS             4095..>4420
                     /locus_tag="VLE22_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13267.1"
                     /translation="MRLAGAAGFAGALKGAEKPVRIGLVGVGSRGTGLLRILLDVPGV
                     QIPAISDINEEHLRNAQDLVEKGNRPRPEGYSGGPEDYRRLVAREGLDAVMTATPWEL
                     HTPVAVA"
BASE COUNT          855 a         1325 c         1411 g          829 t
ORIGIN      
        1 aagtcgtagc cggcgcgtac ccagaattcc acttcgtcgc gcagggtacg gacagggcgg
       61 ccgaggaagg cggccttcac atcctgatgg acaccgaact cgacgatcgg gatgtggctc
      121 ggttcgccct ggcggagaat cgtacgccgg aagttttcga aatcgggttt tctgggggca
      181 agggacgccg ggaccatggc ctcaatcata tcccggaggt tgcgtatggt ttgggccgat
      241 gcttgacggc agcgcacaac ctgatcacaa ttggtcatat atggtcaccg aagtccggat
      301 tgcggaactg aaagcgcgcc ttagcgatta cctgcgatct gtccgccgcg ggaacgaagt
      361 cattgtgaaa gatcgcgaga cgccggttgc gcgaattgtg cctctgcctt cccgtacagc
      421 cggctttgtc acacgccccg cgaccaggac gtttcaggaa gtgggaaaat tgctggcctc
      481 acggccgcgg aagcgcatca agatgaagcc cggcacgctt gagaaggcga tccgggaaac
      541 gaaaaaggac cggcttgact catggacgga tccaaagtct acgtcgactc gtcggtagtt
      601 ctgcggaagc tgcttgacgc gccgggtgca gttccgcact ggggatcctg gacgctggcg
      661 gtggcgagtg aactggtcca agtcgaagcg ctgaggaccc tcgaccgctt gaggattctc
      721 ggggaactca gcgtgacgga atacgccgat tgcgtcgagg aactgggctt atggctgagc
      781 gccttcgaat tggctccgat cgaccgcgcc gtgcttcggc gggcagcagg gccgttttcg
      841 acagccatcg gaacgctcga cgcgattcac cttgcgacgg cgttgctctg gatggaacgg
      901 gagccggctg agctgacgct cgtcacccat gaccgccagc aggcggccgc cgcgcgggcc
      961 tgcgggatcg ggggttcagt tcaacccgta gcgggcctgc tggatgcgcc ctgcggacag
     1021 ggcctcaacc ggttatttcc tttctcgcgt gaagcggctg gcaagccagg atgcgagagc
     1081 gggctctgcc tcggacgggc gcgcgatcag gcccgtcgag gtcttgagcg cagagccgaa
     1141 ttccgtctga agccgcgcat ttccagccag aacgttacgc ccgtcgacga acccctcgat
     1201 caggacgggc cgtgagcttt gggctgcgac gatgtcagcg acgtcggcgg catcgacaat
     1261 cccgggcacg atcacatcct gagggacgtg gctgaagcga tcctcgagca cggagacgaa
     1321 agaaaccaag ccgcctcttg cggcaaccgc ggcgacgtga tcttcataca agccagtcag
     1381 tagtgcaagc agtgcgccca tgggttcggc ctgatgctgg acgaactcac cgggcgtctg
     1441 ctggtggctt tgatcgaagg cgaaatcatc cgcattcatt tccgcgaacg attctcccca
     1501 gagtgcgata tgcgcggggt cgatgtccgg ccgcgaagcc agatagcgaa atacggttcg
     1561 agcgtccttc agccttgcgc caagaagcgt atcgccgagc atgaattcgc tcgccgcaag
     1621 agacatggcg tcgggaccgc gggattgagt gtcggcgagc tctcctatgc cccgcaggtc
     1681 cggcaggcag accgcgatcc cgtttcccag caagctcatc acttcatcgc cgcgtgccgc
     1741 caagaactgc tctttcccgc ccgcggcaat tgcgagaacc gccggcgctc gcgccgccgg
     1801 tttaaccggc ttcaggagca atacgggaag cgtgatcccg ttttcgcttt caatcgccaa
     1861 cgcttcgact gtggcttggg cgccctgctt atcccatagt gcttgaacgg cgggcctctc
     1921 gaccggctca atgtcgccga gtttcgcttt cagcaccact cgcaactggc tccgtcgctt
     1981 ctgctctgga acgatcgaga tcgcagaccg ggattgtgcg aggcgcccgc gagccaattc
     2041 gagtgcgatg gaactggcgg acttcggatt acgctccgct gcaatcgcgg gcgtcagaca
     2101 catcagctcc gattccggac ggttcttgtg atactccacg gcgggaactg agattccgag
     2161 ccagcgcttg aggatggggt atatcccctg gcggagaaat gtgccgacgt tcgtgcactc
     2221 gcccggtccc gggaatggac cgaagccatg gacttcgtcg aggtggtccc gtgccccgta
     2281 aagatcgaag actttcttgt agcgagtcca agctggttcg tgctccacat cctgcggcca
     2341 gccaatctcg aatgaataca ggaaataccg aggcgctacc gaggcgcaga tcatccaggg
     2401 gaagaactgg tcaatggagc tgcgtggcat gcagcgcgtg gtctcccact cgccccagcc
     2461 cggccaggcc gtctcgaagt cgtatccgcg cggacccagt gtgtagtgct cctcggggcc
     2521 ggcttcacca aaattaaacg ggatcacggc tgcaatgcgc ggatcgagcg ccgcagtgac
     2581 cgcggcggga tcgcctcccc cggctacggc gcccagcatg ataatccgtt ttggatcgat
     2641 gtacggcctt tctagcagaa gatcgacact gcgcatcaag tcccagacca tccacttcat
     2701 caggctttcg cccgcaacca gcagttgatt gcccagcgtg taccgcccgt agtaactctc
     2761 gcggtaccac ggctgcgatt gcgaccgttc gcctgcgcac agttgatcca tgacgagcac
     2821 tgctgttcca gaccgcgccc atgtcattcc gagatcctgc aattcggact gcgtctttgg
     2881 agcgtgatga gcatgtgcaa cgataatggc cgggacactt ccggatggaa ctgcgggcag
     2941 gtaaagattc gcggtgacga gcagattcgg acggctctcg aacacgatgt tttcgatcac
     3001 gaagccatcg ccgaaatccg cgcgacgcgt cacaaccgga cgtagaggcg tgcgctcggg
     3061 aaacggctcc agtgattttc gcaatgcccc gacgcgcgga tctcgaaagc gctcccaggt
     3121 ttctcgagaa ttgaccctct gccacgccaa tttctcctgc tcggcgatcg ccgacatgcg
     3181 agcgcgaaga gaattctcaa gcatgccgga cgtgcgcagc tggttgcgct gctcttcggt
     3241 ccagacggtc ttcggaatcc aggcaagagc ggggacgggc ttggtctctg gcctgggagc
     3301 ggcggcgccg agccgttggg cagggcgaaa gtccggtgcg ggctccctgt tgagttccga
     3361 aagctcgaat accgcccgct cattcgcggc cggccagtgc cagcgcaact ccggcgcaac
     3421 cggccggggc gctctgattc gctcgacgcc ggtgaatccg ttctttgcga gccgctcaag
     3481 ggggatagca gcctcgaccg tccattggcc gggatcgcga ttggttgcca ccaggactgt
     3541 ttccgctcct ttgagcggtt cgagcgtgat gctgttctga aaccgccagg cgcccagggg
     3601 attcacggcg agtgagacgt gtacgttgcg gtggagcgca ccgtcataga cacgaaacgt
     3661 ccagatgatg agatcctccg accaccagtc agggttgcgg cccgtcgaat gagcaaccag
     3721 gcgatccggt tccggcattc gcgcgctaag gcacaggtac ccgccacggg cggcgaggcg
     3781 cacctcacca cccccgggga acggcggacc gaattcagaa gaagccagcg cgagcacttt
     3841 cgcgctctgc catgcggggt catccagctt accgtcaatt gccagcgggc cggaaaccga
     3901 cggaacgaga atggacgcgg tttccgcggc aagggcgctt gcgcagcaca gggagagaac
     3961 ccagaggcga cttttattca tgagacggaa tttaccacgt tccacgcagc gcattgcgcc
     4021 cggcgtgaac actgcgtata ctgcgggtga attgaggggg ctgtatgtca ggtgactatc
     4081 aacgtcgtga gttcttgaga ttagcgggtg cggccggctt cgccggtgcg cttaaggggg
     4141 ccgagaaacc ggtgcgcatc ggtttggtcg gcgtgggtag tcgagggacg gggctgctga
     4201 ggattcttct cgacgtgccg ggggtgcaga taccggcgat ttccgatatc aatgaagagc
     4261 acctaagaaa cgctcaggac ctcgtcgaaa aaggcaaccg tccgcggcca gaggggtatt
     4321 ctggaggccc ggaagactac cggcgtcttg tggctcgcga gggcctggat gcggtgatga
     4381 cggcgactcc ctgggaattg cacacccctg tcgcggttgc
//
LOCUS       DATIFQ010000053         3451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1933064, whole genome shotgun sequence.
ACCESSION   DATIFQ010000053 DATIFQ010000000
VERSION     DATIFQ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933064"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1804
                     /locus_tag="VLE22_02350"
     CDS             77..1804
                     /locus_tag="VLE22_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13268.1"
                     /translation="MPFFLPPLLAGEAVLVVLALGFAFLWPLGGSRVFRTFAGRWKQL
                     ARRRWTSLIVVGVLVLVARAALLPFFPIPKPENHDEFSYLLAADTFASGRLTNPTHPM
                     WAHFESFHIIHQPSYMSMYPPAQGLVLAAGRRLFGHPWFGVWLSMGVMCASVCWMLQG
                     WLPPTWALLGGLLVALRLGIFSYWMNSYYGGSVAAIGGSLLLGALPRLRRRPSVACSL
                     CLGAGLAILANSRPYEGLLIALPVAGVIAWLAACRAGRSAFLRKVVAPLFAFLVLLVI
                     AMGYYNQRVLGNPFELPYDANRRMYAVAPDLIWQPQRTEPGYRHAVMRDFYIRHELTF
                     SRVTRTLNGYLGMIPVRLIVFWSFFIGPALTLPLLMLPRVLRDRRMRLLVIIGVVFAL
                     GLAAEVFLLATHRYAPATCLVHALLLQGMRHLRHWRWKNRPIGLTVVVLAPVVCLLML
                     GFRASAGVLGIPSDGIWTWYSSTSRGLQRGHILERLHRLGGRHLILVRYSPDHDYFSE
                     YVYNDADIDRSQVVWAREMDPQSDRELLCYFQDRQIWLLEADQNPPRLSPRPMSSPSL
                     CQTRAQPSP"
     gene            complement(1816..2775)
                     /locus_tag="VLE22_02355"
     CDS             complement(1816..2775)
                     /locus_tag="VLE22_02355"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB13269.1"
                     /translation="MSRTITRRRAIAGLAGATALSSCARKPRPGGEPRVAIIRAAGYT
                     QELYDKVRRLVGQSGLDVRGKRVVVKPNLVEFDPGTSINTNPLLVHAVVEAFRGLGAS
                     DVRIAEGPGHRRVTWDLAEAAGYFAAIPEFERCFTDLNLDDVAKVALRKNESRLGSLY
                     FPKTVLGCDLLVSVPKMKTHHWVCATLSMKNFFGLVPGGVYGWPKNILHWAGIHECIV
                     DLHATFPQAFAIVDGIEGMEGNGPIQGTPKSAGVVVAGSDLAAVDATCCRIMRIDPTK
                     IHYLELASDLGQTTEANVKQIGELIQTVHTEFALLPAFRSLRI"
     gene            complement(2779..>3451)
                     /locus_tag="VLE22_02360"
     CDS             complement(2779..>3451)
                     /locus_tag="VLE22_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13270.1"
                     /translation="APHPSVDQSTWVTALAVLALADGHSREVLQPAVSWLLRQEGKES
                     STLHRLRLWLLGQGSDVDTSQEGWPWYPETAGWVAPTALTILALKRVDRAGIGVDVQD
                     RVQAGRSFLMLRRCADGGWNHGSSRALGYEAGSYPESTGLALLALQEIPAAALTKSFE
                     TAERHFRECRSAEGRSWLRMALLAHSRPVPDSLVESARCRTVVDCSLCVLADAAANGH
                     NLLLG"
BASE COUNT          584 a         1088 c         1013 g          766 t
ORIGIN      
        1 aggttgctgg accttaccga ttgcctcgca gtgcctgctc gggattcacg gtatcatacc
       61 tcaggtaaca ggctgaatgc ccttcttcct tcccccattg ctcgctggcg aggcggtact
      121 tgttgttctc gctctcggct tcgcgtttct ctggcctctc ggcggttccc gagttttccg
      181 cacattcgcc ggccgttgga agcagcttgc gcggcgccga tggacgtctt taatcgtcgt
      241 cggtgtgctt gtcctcgtag ctcgggcagc tctcctgccg ttcttcccga ttcctaaacc
      301 ggagaaccac gatgagttca gctatctcct ggcggcggac actttcgcct cgggacgtct
      361 gacgaatcca actcacccga tgtgggccca cttcgagagc tttcacatca ttcatcagcc
      421 gtcctacatg tcaatgtatc cgccggccca ggggctggtc ctggcggccg gaagacgttt
      481 gtttggacat ccgtggttcg gcgtctggct cagcatgggt gtgatgtgcg cctccgtttg
      541 ctggatgctc cagggctggc ttccccccac ctgggcgctc ttgggtggcc tgcttgtcgc
      601 gttgcgtctc ggcatattca gttattggat gaacagctat tacggcggct cggtggcggc
      661 gatcggtggc agccttttgc tcggcgcgtt gcccaggctg cgccgtcgcc ccagcgtcgc
      721 gtgctcgctc tgtctggggg cagggcttgc aatcctggcg aatagccggc cgtacgaggg
      781 cttgctgatc gcgctgcctg tggctggcgt catcgcctgg ctggcggcat gtcgagccgg
      841 ccgtagcgcg ttcctaagga aagtcgttgc accgttgttt gctttcctgg tcttgctcgt
      901 gatcgccatg ggctactaca accagcgagt cctgggaaat ccattcgaac ttccctatga
      961 tgcaaatcgc cgtatgtacg ctgtcgcacc cgacttgatc tggcagcctc agcgaacgga
     1021 gcctggctat cgccacgccg taatgcgcga cttctatatc cgccatgaac tgactttctc
     1081 ccgcgttacc cgaacgttga acggttacct cggcatgatt ccggtccggc tgatcgtgtt
     1141 ttggtccttt ttcatcggtc ctgcgttgac gctcccgctt ttgatgttgc ccagagtgct
     1201 gcgagaccgg cgaatgcgcc tgctggtgat tatcggagtg gtctttgcgc ttggcctcgc
     1261 tgccgaagtg tttctcctgg cgacgcaccg gtacgctccg gcgacctgcc tcgtgcacgc
     1321 gctactgctc caggggatga gacatcttcg ccattggcgg tggaagaatc gacccatcgg
     1381 gttgacggta gttgtgctgg ctcccgttgt gtgcctgctc atgctcggtt tcagagcatc
     1441 cgccggcgtt ctgggaattc cgtccgacgg catatggacg tggtattcgt caacgtcccg
     1501 cgggctgcaa cgggggcaca ttctggagag actgcatcga cttggcggcc ggcatctcat
     1561 tcttgtccgg tactcacccg atcatgacta cttcagtgag tacgtctata acgacgccga
     1621 catcgaccgt tctcaggtag tctgggcccg cgagatggac ccacagagtg atcgggaact
     1681 tctctgctac tttcaagatc gccagatttg gcttctggaa gccgatcaga atccaccgcg
     1741 cctctccccc cgcccaatga gttccccatc cctgtgtcag acgagggctc agccgtctcc
     1801 ttgagacggg ggcattcaga tccgtaagga tcggaatgcc gggagcaacg cgaattcggt
     1861 atgaacggtt tggataagct cgccaatctg cttcacgtta gcctcggtcg tctgtccgag
     1921 atctgacgcg agttccaggt agtggatttt cgtcgggtcg attcgcatga tccggcaaca
     1981 tgtggcatcc accgcggcca ggtcacttcc cgccacaacg acgccggcgg acttcggcgt
     2041 cccctgaata ggcccgttcc cctccatccc ctcgattcca tcgacgatcg cgaatgcctg
     2101 tgggaacgta gcgtgcagat ccactatgca ttcgtgaatg cccgcccaat gaaggatgtt
     2161 cttgggccag ccgtaaacgc cgcccggaac cagtccgaag aagttcttca tacttagcgt
     2221 ggcgcaaacc caatggtggg tcttcatctt gggcacgctt accagcaggt cgcatccgag
     2281 gacggtcttc gggaagtaca acgagcctaa tcgggattcg ttctttcgca atgccacctt
     2341 ggccacatca tcaaggttca ggtccgtgaa gcaccgttcg aactccggta tggcggcaaa
     2401 gtatccggct gcctctgcga ggtcccaggt aacccggcgg tggcccggcc cttctgcgat
     2461 ccggacgtca ctcgcgccaa gcccccggaa ggcttcgaca accgcgtgaa cgagaagcgg
     2521 gtttgtgttg atcgatgtgc cgggatcgaa ctcgactagg ttcggcttga cgacgacgcg
     2581 tttaccacga acgtccagcc cactctgccc tactaggcgc cgcactttgt cgtagagctc
     2641 ctgcgtgtag ccggctgctc ggatgatcgc gactcgcggc tcgcccccgg ggcggggctt
     2701 cctcgcgcaa ctcgacaagg cggttgcccc tgcgagcccc gcaatagccc gccgccgtgt
     2761 gatcgtccga ctcatggtct acccgagcag aaggttatgt ccattcgccg cggcatccgc
     2821 cagcacgcac agggaacagt cgacaaccgt gcggcaccgg gcggactcca ccagtgaatc
     2881 ggggacgggc cgcgaatgag ccaacagcgc catacgcagc cagcttcggc cttcggccga
     2941 acggcactcg cgaaagtggc gttcagccgt ctcaaaagac ttcgtgagcg ccgcagcggg
     3001 tatttcttga agtgccagca aggcaaggcc ggtagattct gggtaggatc cggcttcata
     3061 gccgagcgca cgagaagaac cgtgattcca cccgccgtcg gcacatctcc gtaacatcaa
     3121 gaagctccgg ccggcctgga cccgatcctg cacgtccact ccgataccgg cgcggtcaac
     3181 tcgtttgagg gcaaggatcg tgagtgctgt cggggcaacc catcccgccg tttccggata
     3241 ccacggccac ccctcctggc ttgtgtcgac gtcggagccc tggcccagca gccagagccg
     3301 cagccggtgc aacgtgcttg actcctttcc ctcttgccga agcagccaag agacagcggg
     3361 ttgaaggacc tcacgggagt ggccgtctgc gagcgccagc accgcaagtg ccgtgaccca
     3421 cgtgctttgg tctacagatg ggtgaggcgc c
//
LOCUS       DATIFQ010000054         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1166869, whole genome shotgun sequence.
ACCESSION   DATIFQ010000054 DATIFQ010000000
VERSION     DATIFQ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166869"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="VLE22_02365"
     CDS             complement(<1..1521)
                     /locus_tag="VLE22_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB13271.1"
                     /translation="MVPSTLLGVVVRFGVFEFDLKSGELLKQGRRVRLSGQPAQILAF
                     LLRRPGELVTREELQQALWPADTHVNFEQSLNAAVKRLRHALGDSPEKPLFIETMARR
                     GYRFIAPASAPGQSALPSTKPVHEVNSIAVLPFENAMADPDADYLVDGLAEAIINALS
                     RLPALRVLARSTVFRYRGKLVDYRALGRKLNVGAVLSGRVSRRGDELMIGTELVEVQS
                     GCLIWGEQFSRKLSDVLLIEAELSAKISEKLRSELAGKANVFATARQHTQSTEAYQDY
                     LKGRYHWNRMSAEGLQRSIDYFQHALQKDPGFALAHAGLADSYCLLGFFDLLPPAEAM
                     PKAKEAAIRALEIDGDLAEAHASLANVLKVYDRDWLAAERRYQQALQLNPNYVHAYRG
                     YAALLAATGRFSESIPQIRHANELDPLSIVVSMEMAWNFFIARDYDHAIEQALRVTHF
                     EQEFPSAQFILGLACEQKGRFEEARAALERTLAGSHGHASAPASLGHLFGVTGQREG"
     gene            1802..>2069
                     /locus_tag="VLE22_02370"
     CDS             1802..>2069
                     /locus_tag="VLE22_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSB13272.1"
                     /translation="MSTFAFGLETEPVPMQAGLDPRNVAALETLAMSIVHDLRNPLAA
                     IHNGAEILNRSQLPEQQVRRLTRNMYNASVRIQELLQDYVDLCRT"
BASE COUNT          422 a          627 c          623 g          397 t
ORIGIN      
        1 tccctccctc tggccggtga cgccgaagag gtgcccgagg ctggccggtg cggacgcatg
       61 gccgtgggag cccgcgagag tccgttcaag cgcggcgcgc gcctcctcga atctcccctt
      121 ctgctcgcac gcgagcccca gaatgaattg cgcggacgga aactcctgct cgaagtgggt
      181 gacgcgcaac gcttgttcga tggcatggtc gtagtctcgg gcgatgaaga agttccaggc
      241 catctccatg ctgaccacga tggacagcgg gtccagttcg ttcgcgtgcc ggatctgggg
      301 aatggactcc gagaagcgcc cggtcgccgc gagtagcgca gcatagccac gataggcatg
      361 aacgtagttc ggattcaact ggagcgcctg ctggtaccgc cgttccgcgg cgagccagtc
      421 gcggtcatac actttcagga cgttggcgag ggaggcgtgc gcttcggcga gatcgccgtc
      481 gatctcgagg gccctgatgg cggcctcttt ggccttcggc attgcttcgg cgggcggcaa
      541 caggtcgaag aagcccagga ggcaatagga atccgcaagc ccggcgtgag ccaacgcaaa
      601 acctggatcc ttctgtagag cgtgctggaa gtaatcgatg cttctttgta acccttcggc
      661 cgacatcctg ttccagtgat acctgccttt caggtaatcc tggtacgctt ccgtgctctg
      721 cgtgtgctgt cgcgcggtgg cgaagacgtt cgctttcccg gccaattcgg aacggagctt
      781 ctcggagatt ttggccgaca gttcggcctc gatcagaaga acatctgaaa gcttgcgact
      841 gaactgctca ccccagatca ggcacccact ctggacttcc accagttcgg ttccgatcat
      901 cagttcatcg ccgcgccggg agaccctgcc ggaaagaaca gcgcccacgt tcagcttccg
      961 tccgagcgcc cggtaatcca ccagcttgcc ccgatagcgg aagaccgtgc tccgggccag
     1021 cacccgaaga gcaggcagtc tcgaaagcgc gttgatgatg gcctcagcga ggccgtcgac
     1081 cagatagtcg gcatccggat cggccatcgc attctcgaag ggcaacaccg caatggaatt
     1141 gacttcgtgg accggcttgg tcgatggaag agctgactgg cccggagcgc tggcgggagc
     1201 gatgaaacgg taaccgcgac gcgccatcgt ttcgatgaag aggggcttct cgggcgagtc
     1261 acccaacgcg tgacgtagcc gtttcacggc cgcattgagg ctctgctcga agttgacgtg
     1321 cgtatctgcg ggccagagcg cctgctggag ttcttcacgg gtgactagtt ccccgggacg
     1381 ccgcagcaga aaagctagta tctgggccgg ctggcccgaa agacgcaccc gtcgcccctg
     1441 cttaaggagt tcccccgatt tgaggtcgaa ttcgaaaacg ccgaatcgaa ctactacacc
     1501 cagaagcgtt gaagggacca tccggcacat ccctggcagg agaatccatg cacctagccg
     1561 cacccagtaa actgaggatc ccccgtctac caagggagta tagcacagcg ccgggtcggt
     1621 catagttccg tgtaatcttc tggtgtaaaa gtagttccgg tgtcacaaac gtttatctgc
     1681 aatgtcactc gattgctatt gaagtacacc tcagaacaga agaaactgct gatgtgggct
     1741 aggagccgac ctgggaagcc ggtctgccca agacgacccg ccgaacgagg agttgtgttt
     1801 gatgagcacg tttgcgtttg gccttgaaac cgagccggta ccgatgcaag ctggcctgga
     1861 cccgcggaat gtcgctgccc tggaaactct cgccatgtcg attgtgcacg acctccgcaa
     1921 cccgctcgcc gccattcaca acggggcaga gatactgaat cgctcgcaac tccccgagca
     1981 gcaggtccgg cgcctcacgc gcaacatgta caacgcctcg gttcgcatcc aggaactgct
     2041 ccaggattat gtcgatctat gccggacga
//
LOCUS       DATIFQ010000055         6983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1741834, whole genome shotgun sequence.
ACCESSION   DATIFQ010000055 DATIFQ010000000
VERSION     DATIFQ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741834"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VLE22_02375"
     CDS             complement(<1..1158)
                     /locus_tag="VLE22_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HSB13273.1"
                     /translation="MPVTGSKRFEARRERPVNRDSYIHEWPEAGLVMFDSPCDPRPGV
                     RVEGGRIVEMDGRREAEFDMLDRFIARYAIDPDMAAEAMSMDPLEIARMMVDIRVPRR
                     KVVRMVSGLTPAALAGVVNRLNVVEMMITLQKMRARRTPSNQAHVTNRKENPALLAAD
                     AAEAVERGFAELETTVGVARYAPLNALAILIGSQTGRGTALTQCAVEEALGLKLALAG
                     LTTYAETLSVYGTEQAFRDGDDTPWSKTFLAAAYASRGIKVRFTSGSGSEALMGHAEG
                     KSMIYLETRCLMAIKGAGSQGVQNGSISCIALPEALPGGVRGVLAENLIAALLDLEVA
                     SGNDAMASHSEIRKTAKLMLQFLPGTDFIFSGYSSMPREDNLFGGGNFDTED"
     gene            1207..2325
                     /locus_tag="VLE22_02380"
     CDS             1207..2325
                     /locus_tag="VLE22_02380"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSB13274.1"
                     /translation="MKRLFLVPAIPMFAVALAVAAEEDIKPLAIGAKAPDFSLPGVDG
                     RNHSLADFAKSKLLLIVFTANHCPTAQYYEERLKRIVSDYKGKGVAVVAISPNDPHSV
                     RLDELGYTDLADSFEEMKIRAAHKKFNFPYLFEGDSTGISRAYGPTATPHAFLFDSER
                     KLRYVGRIDDSEREQYVKTRDLRNALDALLAGREVPVAQTRAFGCSIKWAGKADSVKK
                     FMAKLAAEPVSVEPVDAAGLKALRKNDSGKLRLVNFWATWCGPCITEFPELIEINRMY
                     RHRAFEFVSVAANFPDEKNEVLAFLKKNEASNRNLLFADTDKYKLMEAFDAEWNAALP
                     YSMLIAPNGEVLYKIQGAIDPLELKRTIVRSLKEDRFK"
     gene            complement(2332..3510)
                     /locus_tag="VLE22_02385"
     CDS             complement(2332..3510)
                     /locus_tag="VLE22_02385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSB13275.1"
                     /translation="MARRLQPVTSPLAETFRRFGRGVRRNGVPLRRGVSRDSSTGGVV
                     GRVISHYRVLDRIGKGGMSVVYKARDLKLDRFVALKFLPPHLAESEEAVARFLREARA
                     ISSLNHPHIATIYDLKQTHEGLFLVLEYLPGGTVRSLVRELAAAGERLSLRQLFEFAA
                     GITEGLAHAHRQGVIHRDVKTDNVMLTAEGSAKLTDFGLAKLQDGCRLTRSGSIVGTA
                     SYMSPEQARGEELDERSDIFSLGVVLYEMATAQLPFRGPHEVAVLSEVLYSNAPNVCR
                     LRGDLPSAFGEIVRKAIEKKPERRYQSACELLVHLRTAAGDPGSGITAVSADAETKPL
                     TPLPRPGKARRPILRALPRWLRVAILLGLVASPAIVVPSFRAEIVNRLIPAQTANPGK
                     "
     gene            3630..4157
                     /locus_tag="VLE22_02390"
     CDS             3630..4157
                     /locus_tag="VLE22_02390"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HSB13276.1"
                     /translation="MRWLRQRAWSPYVAGALIGVLSWFAFLTADEPLGVSTAIVQTVA
                     FAERMIVPEHIQQNQYFAKIPQVVNWGWVLVVGLMLGAFVSSRLSGEESGEKVPLVWR
                     ERFGNSVKKRYFGALLGGFLIIFGARMAGGCTSGHGISGSLQLAVSGWTFFLSLFVSG
                     VLTALVLYGKGDRDV"
     gene            4150..5007
                     /locus_tag="VLE22_02395"
     CDS             4150..5007
                     /locus_tag="VLE22_02395"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HSB13277.1"
                     /translation="MFEPAPRLLLGLLTGFLFGFFLQKARVTKFDVIVNQFRFKDFTV
                     LKVMLTAIVTGGAGVYFLRFEGLANLHVKPAQMAGVVLGGLIFGVGMAVLGYCPGTGL
                     AAAAEGSRHALWGVLGMLLGAAGFAEMYAFLSGNVLKWADFGKITLPDVTGIPAWAWL
                     AGLALVAAAVFALIEKFERRGSRAEREKRRRLAVNARSRLAEATVADAREDTLFYSYT
                     VRGVQYSTSQDVTDLHDYLPGEPGMLVGPATVKYVPENPANSILVCEEWSGLRRYDAD
                     RSHTTTERS"
     gene            5018..5413
                     /locus_tag="VLE22_02400"
     CDS             5018..5413
                     /locus_tag="VLE22_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HSB13278.1"
                     /translation="MKKTLGNWKLMAAGFVALAMFGAGFLAGANTFNKPSTVLHLVTV
                     KWTADSNPEQRKAAIDGIEKMASEVPGIKNIWLKPVRVQPRDYNAVFAIEFENQAAAD
                     RYAKHPAHEAWYKVYEPVREESRSQQVTN"
     gene            complement(5468..5671)
                     /locus_tag="VLE22_02405"
     CDS             complement(5468..5671)
                     /locus_tag="VLE22_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HSB13279.1"
                     /translation="MKEKGTVKWFNAAKGYGFIQRSSGEDVFVHHSAIQMDGYRALDN
                     GVEVEFEVKQGPKGLQAENVTAV"
     gene            5946..6086
                     /locus_tag="VLE22_02410"
     CDS             5946..6086
                     /locus_tag="VLE22_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13280.1"
                     /translation="MRTKRKPQGDIELAYYTQVALIMAMRSFVERKVATFDATREEIQ
                     MS"
     gene            6156..>6983
                     /locus_tag="VLE22_02415"
     CDS             6156..>6983
                     /locus_tag="VLE22_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB13281.1"
                     /translation="MDPALQGSRPDADGAALNISNRRRWGIVGLLFSASMINYMDRAT
                     VSMALPFISADLGLDPTAKGELLSAFFASYALMQIPIGWCADRFNLKWLYAVMFLLWS
                     LAQGLSGFAVSLAMLMTFRVMLGIGESIYLPGGTKIVSLLFPPANRGLPSGLFDFGTR
                     TGLVLDGILIPWMIVHYGWRRMLMLVGFSALLWLIPWIFVFPSHVRSPKRTDHLQPRV
                     SFWRGAWVLMSNRNLNGICLGFFCFDYYWYLFVNWLPDYLVTVRHLTILKAGFFASLP
                     "
BASE COUNT         1252 a         2251 c         2096 g         1384 t
ORIGIN      
        1 gtcttcggtg tcgaagttgc cgccgccgaa caggttgtcc tcccgcggca tcgagctgta
       61 acccgagaag atgaagtccg tccccggaag aaactgaagc atcagcttcg ccgtcttccg
      121 gatctcggaa tgcgacgcca tcgcgtcgtt gcccgacgct acctcaaggt ccagcagagc
      181 cgcgatcagg ttttccgcga gcaccccccg caccccgccc ggcagcgctt ccggcagcgc
      241 gatgcaactg atcgatccgt tttgcacgcc ctgcgatcca gcgcccttga tcgccatcag
      301 gcaccgcgtc tcaaggtaga tcatcgactt cccctcggcg tgccccatca gcgcctccga
      361 gccgctgccc gaagtgaacc gcaccttgat cccgcgtgac gcgtacgccg ccgccagaaa
      421 cgtcttcgac cacggagtgt cgtcgccgtc ccggaacgcc tgctccgtgc cgtaaaccga
      481 cagcgtctcc gcgtaggtcg tcagtcccgc cagcgccagt ttcagcccca gcgcctcctc
      541 caccgcgcac tgcgtcagcg ccgtcccccg gcccgtctgc gagccgatca gaatcgccag
      601 cgcattcagc ggcgcatagc gcgccacccc caccgtcgtt tcaagttcgg cgaacccgcg
      661 ctccaccgct tcggcggcgt cggcagccag cagcgccggg ttctccttcc ggttcgtcac
      721 gtgcgcctga ttcgacggcg tccgccgcgc cctcatcttc tggagcgtga tcatcatctc
      781 gacgacgttc agccggttca ccacccccgc cagcgccgcc ggcgtcagcc cgcttaccat
      841 ccgcacaacc tttcgccgcg gaacgcggat gtccaccatc atgcgcgcga tctccagcgg
      901 gtccatcgac atcgcctccg cggccatgtc cgggtcgatg gcgtagcggg cgatgaagcg
      961 gtccagcatg tcgaactccg cctcccgccg gccgtccatc tccacaatgc gtccgccctc
     1021 cacgcgcacc cccggccgcg gatcgcacgg gctgtcgaac atgactaggc ccgcttcggg
     1081 ccactcgtgg atgtagctgt cccggttcac cggacgctcg cgccgcgcct cgaaccgctt
     1141 cgatcctgtc accggcattc ccccattctt gcccactggc gtcccatttt cagtaccctg
     1201 tacataatga agcgactttt ccttgtgccg gcaatcccaa tgtttgcggt cgcgctcgcg
     1261 gtcgccgccg aagaagacat caagccactt gccatcggcg cgaaggcgcc ggatttcagc
     1321 ctgcccggcg tcgatggccg caaccactcg ctcgcggact tcgcaaagtc gaaactgctg
     1381 ctcattgtct tcacggcgaa tcattgcccc accgcgcagt attacgagga gcgcctcaag
     1441 cgcattgtca gcgactacaa aggcaaagga gtcgcggtcg tcgccatctc ccccaacgac
     1501 ccgcattctg tccgcctcga cgagctcggc tacaccgacc tcgccgattc gttcgaggag
     1561 atgaagatcc gcgccgcgca caagaagttc aattttccgt atctgttcga aggggacagc
     1621 acgggcatct cgcgcgccta cggtcccacc gccacgccgc acgcattcct gttcgattcg
     1681 gagcggaagc tgcgttacgt cggccgcatc gacgacagcg agcgggaaca gtacgtaaag
     1741 acgcgcgatc ttcgcaacgc gctcgacgcg ctgctcgccg gccgcgaggt gcccgtggcg
     1801 caaacgcgcg cgttcggctg ctccatcaag tgggcaggca aggcggactc ggtgaagaag
     1861 ttcatggcga agctcgccgc cgagccggtc tcagtcgagc cggtcgacgc cgccggtctg
     1921 aaggccttgc gcaagaatga ctcaggcaag ctgcggctgg tcaacttctg ggccacctgg
     1981 tgcggtccct gcatcacgga gttccccgag ctgattgaga tcaaccgcat gtaccggcac
     2041 cgcgcgttcg agttcgtctc ggtcgccgcc aacttccccg acgagaagaa cgaggtgctc
     2101 gcgttcctga agaagaacga ggcatccaac cgcaacctgc tcttcgcaga caccgacaag
     2161 tacaagttga tggaggcgtt cgacgcggag tggaatgcgg cgcttcccta ttcgatgctg
     2221 atcgccccca acggtgaggt gctctacaaa atccagggcg cgatcgaccc cctcgagctc
     2281 aagcgcacca tcgtccgctc gctcaaggaa gaccgcttca aatagcgccg ctcacttccc
     2341 agggttggcc gtctgcgccg gaatcagccg gttcacgatc tcggcccgga aggaagggac
     2401 cacaatcgcc ggagacgcca ctaaccccag cagaatcgcc acccgcaacc aacggggaag
     2461 ggctcgcagg atcggccggc gagcctttcc cggcctcggc aatggcgtca acggcttggt
     2521 ctccgcatcc gcggaaaccg ccgtgatccc gctgcccgga tccccggcgg cagttcgcag
     2581 gtgcaccagt aattcacacg cgctctgata ccggcgctcg ggcttcttct cgatcgcctt
     2641 ccgcacgatc tctccgaacg cggaaggaag gtcgccgcgc agccggcaca cgttcggcgc
     2701 gttgctgtac agcacctcgg agagcaccgc cacttcatgc ggcccccgaa acggcaactg
     2761 cgcagtggcc atttcgtaca gcacgacccc gagggagaag atgtccgatc gctcgtccaa
     2821 ctcctccccg cgcgcctgct ccggcgacat atacgacgcc gtcccaacga tgcttcccga
     2881 acgcgtcagc cggcacccgt cctgcaattt cgccagcccg aagtccgtca gcttcgcgct
     2941 gccttcggcc gtcagcatca cgttgtcggt cttcacgtcc ctgtggatga cgccctggcg
     3001 atgtgcgtga gcgaggcctt ccgtgatgcc ggccgcgaac tcgaacaact gccgtagcga
     3061 cagcctctcg cccgccgccg ccaactcccg caccaacgaa cgcaccgtgc ccccaggcag
     3121 gtactccagc acaagaaaca gcccttcgtg cgtctgtttc aggtcataaa tggtagcgat
     3181 gtgcggatgg ttcagggaag aaatcgcgcg cgcctcgcgc agaaaacgcg ccaccgcttc
     3241 ctcgctctcc gccaggtggg gcggcaggaa cttgagcgcc acgaaccggt ccagcttcag
     3301 gtcgcgcgcc ttgtagacga cgctcatgcc acccttgccg atccgatcga gcacacggta
     3361 gtgcgaaata acgcggccga cgacgccgcc cgtcgagctg tctcgcgaga cccctctgcg
     3421 gagcgggacg ccgtttcgcc ggactccgcg gccaaagcgc cggaacgtct cggcgagtgg
     3481 ggatgttacg ggttggagtc tacgtgccac ttcactctgg tagtgcgctt ctcccagaaa
     3541 gcgtttccgg tactgggggg attattttca atgatatagt acccatggaa ccttgacgcg
     3601 ctctgctaca ctgaagtcgt ttcactccaa tgaggtggct cagacaacgt gcgtggtctc
     3661 cgtacgtcgc gggggcgctg atcggcgttt tgtcgtggtt tgcgttcctg acggcggacg
     3721 aaccgttggg agtttcgacg gccatcgtgc agacggttgc cttcgccgaa cgcatgatcg
     3781 ttccggaaca tattcaacag aatcaatatt ttgcaaagat tcctcaagtc gtgaactggg
     3841 gttgggtgct ggtggtgggg ctcatgctcg gggcgttcgt cagctcacgg ctttcgggcg
     3901 aggagagcgg tgagaaagtg cccctggtgt ggcgcgaacg gttcggaaac agtgtcaaaa
     3961 agcgctattt tggtgcgctt ttgggcggtt ttttgatcat tttcggcgcc agaatggcgg
     4021 ggggctgcac gagcgggcac ggaatcagtg gctcgctcca gcttgcggta tccggctgga
     4081 cgtttttcct gtcgctgttc gtctccggcg tgctgaccgc cctcgttctg tatggaaaag
     4141 gagaccgcga tgtttgagcc ggcgccccgg ctgctgctcg gcctcctgac ggggtttctc
     4201 ttcggctttt tcctccaaaa agcgcgggtc acgaaattcg atgtcatcgt gaatcagttt
     4261 cggttcaagg atttcaccgt cctgaaggtg atgctgaccg ccatcgtcac cggcggcgcc
     4321 ggagtttatt tccttcggtt tgagggactt gcaaacctac acgtgaagcc ggcgcagatg
     4381 gccggcgtcg tcctcggagg cctgattttc ggggtgggga tggcggtgct cggctactgc
     4441 ccggggacgg ggctggctgc cgccgccgag ggcagccggc acgccctctg gggcgtgctt
     4501 ggaatgctgc tcggcgccgc gggctttgcc gaaatgtacg cgtttttgag cggaaatgtg
     4561 ctcaaatggg ccgatttcgg caagattacg ctgccggatg ttaccggcat ccccgcgtgg
     4621 gcatggctcg ccgggctggc gctggttgcg gcggcggtct tcgcgctgat agaaaagttc
     4681 gagcggcggg gctcacgcgc cgagcgggag aaacgcaggc ggctagccgt gaacgcgcgg
     4741 agccggctgg ctgaggctac agtcgcggac gcacgtgaag acacgttatt ctattcctat
     4801 actgtgcggg gcgtgcaata ctcgacatcc caagacgtga cggaccttca cgattatctg
     4861 ccgggcgaac ccgggatgct tgtcggtccg gcgacggtga agtacgtccc ggagaacccg
     4921 gcgaattcga ttcttgtgtg cgaggagtgg tccgggctgc ggcgatacga tgccgatcgt
     4981 tctcatacaa ccactgaaag gagctaaagg agaagagatg aagaagacac tgggaaactg
     5041 gaaactgatg gccgcgggtt tcgtggcgct tgcgatgttc ggcgcgggct tcctcgcggg
     5101 cgcgaacaca ttcaacaaac cgtcgacggt cctgcatctg gtgacggtga agtggacggc
     5161 cgattcgaac cccgagcagc ggaaggccgc catcgacggc atcgagaaaa tggcgtccga
     5221 ggttccgggg atcaagaaca tctggctgaa gccggtccgg gtacagccgc gggattacaa
     5281 cgcggtgttc gcgatcgagt ttgagaacca agcggcagcc gaccgctacg ccaagcatcc
     5341 ggcgcacgaa gcctggtaca aggtgtacga gccggtccgc gaagaaagcc gcagccagca
     5401 ggtcaccaac tgagggaatc gggatagaaa aggcgccgta cccttcgggg gccggcgcct
     5461 ttgtttttca gaccgccgtg acgttctcgg cttgcaggcc cttggggccc tgcttgacct
     5521 cgaactcgac ttccacgccg ttgtcgaggg cgcggtatcc gtccatctgg atcgcgctat
     5581 ggtgcacgaa cacgtcctcg ccgctggagc gctggatgaa gccatagccc ttggcggcgt
     5641 tgaaccattt cacagtacct ttttccttca cttctgaact ctcctgaaca aatggtggct
     5701 cgcgaaatga tcgcgaacgg cggcgcgaat cgagggaccc ttcaagacaa gaaaagctac
     5761 gagatctcct cgtagctcag ccctgcttcc gaccaccgcg agaccgcatc ggataccaca
     5821 cctgcgttga actgccttga aattgtctca gaacactgag tttaggtcaa gggttggttt
     5881 gcggcgccgg ttggtgtgga cctggcgcgc aacggttgac gacttgcgtc acggagccgg
     5941 tgatcgtgcg aacgaagcgg aagccacaag gcgacatcga actcgcctac tacacacagg
     6001 tggcgttgat catggcgatg cggtcattcg tggagcgcaa ggtagcgacc ttcgacgcga
     6061 cccgcgagga gatccagatg tcgtgaggga aggtctcgac ccttggttgc tcgccgatgg
     6121 cttcggccct gctaataaga agctaaaatg actcggtgga tcctgccctg cagggctcac
     6181 ggccggatgc cgatggcgct gcattgaaca tctcgaaccg ccgccgctgg ggcatcgtcg
     6241 gcctgttgtt cagcgcctcg atgatcaact acatggatcg cgcgacggtc tcgatggcgc
     6301 tgcccttcat ctccgccgac ctcggactcg acccgacggc caaaggtgaa ttgctctcgg
     6361 ctttctttgc ctcttacgcg ttgatgcaga ttcccattgg ctggtgtgcc gaccgcttca
     6421 atttgaaatg gctctatgcg gtgatgtttc tcctgtggtc gttggcacag ggactttcgg
     6481 gtttcgcggt aagcctggcc atgctgatga cgttccgagt gatgctcggc atcggcgaat
     6541 cgatttatct gccaggaggc acgaaaatcg tcagcctgct cttcccgcct gccaaccgtg
     6601 gccttccttc agggctgttc gatttcggca cccgcaccgg gcttgtgctg gatgggatcc
     6661 taattccctg gatgatcgtc cactacggct ggcgtcggat gctgatgttg gttggatttt
     6721 ccgcgctgct gtggctgatc ccctggatct ttgttttccc gtcgcatgtg cgctcgccaa
     6781 agcggaccga tcatttacag ccgcgcgtct cgttctggcg cggcgcgtgg gttctgatga
     6841 gcaaccgcaa cctgaatggg atctgcctgg gcttcttttg ctttgactac tactggtatc
     6901 tgttcgtgaa ctggctaccg gactacctgg tcaccgtgcg ccaccttacg atcctgaagg
     6961 cgggattttt cgcctccctt ccc
//
LOCUS       DATIFQ010000056         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1550038, whole genome shotgun sequence.
ACCESSION   DATIFQ010000056 DATIFQ010000000
VERSION     DATIFQ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550038"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VLE22_02420"
     CDS             complement(<1..666)
                     /locus_tag="VLE22_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13282.1"
                     /translation="MARTSPWPTVLGTAALIALPVLAAAQTGQAGSTGSQTPQTPTAQ
                     SDKAYPSTAGAAVQHLTEAKQVLDGIPRNAVTGRNATKLNQIRREFEALQRSYSANPS
                     HSGSAHSSSATTTSSKGATGNWNKQLMALDRDLTALLGASSDSTSPETSGATGTSGTS
                     ASSPSAGSASSASSSSLDPNVRSQLEEFRKHITEFAAAASGTQAGGEMSSADTTGSTG
                     TSGT"
     gene            complement(777..1565)
                     /locus_tag="VLE22_02425"
     CDS             complement(777..1565)
                     /locus_tag="VLE22_02425"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HSB13283.1"
                     /translation="MPIPTIRVGSWKELNELVYEGSWNPSLHRHRSTLAFRGMQDAAD
                     DLRTSLHRLGGPTSRLEGHLLRNFRKYAQRETVPDGGMWNWLAVAQHHGLPTRLLDWT
                     YSPFVAMHFATDNVDKFDTDGVIWAIDYDRTNELLPEVLKQQLHEEGSYVFTAEMLSR
                     VASTQHELDTLTPSEFLLFFEPPSLDNRIVNQFALFSLLSSPEARLDRWLERLPDAGH
                     RLVIPAALKWEIRDKLDQANINERVLFPGLEGLSRWLKRYYMPR"
     gene            complement(1636..2037)
                     /locus_tag="VLE22_02430"
     CDS             complement(1636..2037)
                     /locus_tag="VLE22_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13284.1"
                     /translation="MAKKRSGTKRAPSRGRKTGGRKGGRKGAAKRGVRKTSRAARKGR
                     AKTSRGSRKGGTARKSAAKGGAKTRKGAAKKGGARKSATRRASRPARQAMALAGGEEA
                     TMTPMAEPSADLGMPPTTDAELLGDTDEDEM"
     gene            2148..>2679
                     /locus_tag="VLE22_02435"
     CDS             2148..>2679
                     /locus_tag="VLE22_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13285.1"
                     /translation="MLKRVHPVLAVAIEKRDVLAVETTPDSRAVVFSFRAEIRHLRRR
                     FGGRGELWPAGRGRAPFLASLVFSSHMSGIRVAMVSPLCRRVMDPRAAVLFTILIVAA
                     SAVFAGRVQQKMRDFEVYWTAGARVAASQSLYRESDGHYRFKYLPAFAVAVSPLARLP
                     LGTAKAVWFAVSVACLV"
BASE COUNT          398 a          821 c          894 g          566 t
ORIGIN      
        1 cgtcccggag gtccctgtcg atccggtggt gtcggccgag ctcatctcgc cgcccgcctg
       61 cgttccgctg gccgcggcgg cgaactcggt gatgtgcttg cggaactcct cgagctggct
      121 tctcacgttc ggatcgagtg aagacgaaga cgcgctcgaa gcgcttccgg ctgacgggct
      181 cgacgcgctg gtgcccgagg tgccggtcgc accgctcgtt tcaggactgg tgctgtcgct
      241 gctcgcgccg agcagggcgg tcaggtcgcg gtcgagcgcc atcaactgct tgttccagtt
      301 cccggtggct cccttgctcg aagttgtggt cgctgacgat gagtgcgccg acccgctgtg
      361 ggaaggattc gcggagtacg aacgctgcaa ggcctcgaac tcacgtcgaa tctgattcag
      421 tttggttgcg ttgcggcctg tgaccgcatt gcggggaata ccgtcgagca cctgcttggc
      481 ttccgtcagg tgctggaccg ctgcgccggc ggtgcttgga tatgccttgt cgctttgcgc
      541 ggtgggtgtc tgcggcgtct gcgatccggt gctgcccgcc tggccggtct gcgcggcggc
      601 gagaaccgga agcgcgatga gcgccgcggt gcccaacacg gtgggccatg gtgacgtgcg
      661 tgccatcgat gctcctcctc ctcgttgcac gatgtgaatg ttgcctcgcg aggaaagcag
      721 caatgacggt gccagcgagc acgacgagct gagcaggaga agctagcgct ctgccgttag
      781 cgtggcatgt aataccgttt caaccagcgg ctgagtccct cgaggccggg gaagagcacg
      841 cgctcgttga tattggcttg atccagcttg tcgcggattt cccacttgag ggctgccggg
      901 atgaccaggc ggtgccccgc atcaggcagc cgctcgagcc agcggtccag tctggcttca
      961 ggactcgaca gtaaagaaaa gagggcgaac tggttcacga tccggttgtc gagagatgga
     1021 ggctcgaaga acagcaggaa ctctgaaggt gtgagtgtgt ccagctcgtg ctgcgtcgag
     1081 gcgacgcgtg aaagcatctc ggcggtgaag acgtacgatc cttcctcatg cagctgttgt
     1141 ttgagcacct ccggcagcaa ctcgttggtc cggtcgtagt cgatggccca gatcacgcca
     1201 tcggtgtcga acttgtccac gttgtcggtc gcaaaatgca tcgcgacgaa tggggagtac
     1261 gtccagtcga ggaggcgagt gggcaagccg tggtgctgtg cgacggcgag ccagttccac
     1321 atgcctccat ccggcacggt ctcgcgctgc gcgtacttcc tgaagttccg cagcaggtga
     1381 ccctcgaggc gggacgtggg gccgccgaga cgatggaggc tcgtacgcag gtcgtcggcg
     1441 gcgtcctgca tgccccggaa cgccagggtg gaccggtgcc gatggaggct cggattccag
     1501 gagccttcgt agaccagttc gttcagttcc ttccagctgc cgacgcggat ggtggggatg
     1561 ggcacgggga tccttgtcaa ggacaaagga cgaagggaca aaccacaaag aggtcctctg
     1621 tccttgacag gctctttaca tttcgtcttc gtccgtgtcg cccaacagtt cggcgtcggt
     1681 cgtggggggc atgccgagat cggcagacgg ttcggccatc ggggtcatcg tggcctcttc
     1741 accgccggcc aaggccattg cctggcgtgc cgggcggctc gcgcggcgag tcgccgactt
     1801 gcgggcgcca cctttctttg cggccccttt tcgggtcttt gcgccccctt ttgcggccga
     1861 tttgcgagcc gttccccctt tccgggagcc gcgggaggtc ttcgccctgc ccttgcgcgc
     1921 agctcgcgac gtcttccgca cgccgcgctt tgcggcacct ttcctgccgc cctttcggcc
     1981 gcctgtcttc cggccccggc tcggcgcgcg cttagtacca gaccgtttct tcgccatgag
     2041 gtcctccaaa tggggccggc gtacaccgca cccgggcgga tgatagccga actcaacttg
     2101 agcgtccaac ccagcgccgg taatgcggcc gtggaaatcc gttgtcgatg ctgaaacggg
     2161 tccatccagt ccttgccgtg gcgattgaga agcgcgacgt gctcgccgtc gagacgactc
     2221 cagattcgag agccgtcgta ttttcatttc gtgcagagat tcgccacctt cggcggcgat
     2281 ttggcgggcg tggcgagctg tggccagcag gaaggggcag ggcgccgttc ctggcatctt
     2341 tggtattctc ctcgcacatg tccgggatcc gtgtggcgat ggtatctccg ttatgccgcc
     2401 gggttatgga cccgcgcgcg gccgtgttgt tcaccatcct catcgtggcc gcttctgccg
     2461 tttttgccgg ccgcgtacag cagaagatgc gggacttcga ggtgtactgg acggctggtg
     2521 cgcgggtcgc tgcttctcag tcgctctatc gagaaagcga cggtcactac cgtttcaagt
     2581 acctgccagc ctttgccgtt gctgtctcgc cgctcgcgcg ccttcctctc ggcaccgcca
     2641 aagcggtctg gtttgcggtg tcggtcgcct gcctggttg
//
LOCUS       DATIFQ010000057         2174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_784367, whole genome shotgun sequence.
ACCESSION   DATIFQ010000057 DATIFQ010000000
VERSION     DATIFQ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784367"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1443
                     /locus_tag="VLE22_02440"
     CDS             280..1443
                     /locus_tag="VLE22_02440"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HSB13286.1"
                     /translation="MVQVNDLSRSLAAFDPISTLVVVVEMSKASWLVSSVVPGVERQP
                     LKKLEPDATAVLRLIERWRNEAVRVGRPISRIALAYEAGRDGFWLARWLIARGIEVHV
                     IHSASVAVSRERKRAKTDRLDAAMLMRVFLGWLRGERGHCGMVAIPTMEEEDARRPNR
                     ERESLVKERSRIINRMKSALARLGIRGFKPHLRKAPERLAGLRTAEGTGLPANIIEEF
                     RRDMARLALVREQISSIEKTRAERLERAPDTGPHAMVRLLARVIGIGIETADMLVREI
                     LSRKLRDRRALARYAGLTGSPDESGLKSREKGLAKAGNARVRRGLIQLAWRFLMFQKD
                     SALARWYRTRTEGPSGARKTTMIVALARKLLIALWRLVTTGEVPDGVELRTAA"
BASE COUNT          431 a          590 c          792 g          361 t
ORIGIN      
        1 catagtagct gtgaagccga cgaacaaagc gaggaaagcc ccctgcggag gcgtctgcgg
       61 gggcggtcgc agcggtgtcg gtggagcgaa gggcgggggc caaggggaat acgcaccagc
      121 aaagcacgta ctggactcag aaccaggctc gcgtgtcaca ggcgctggag cgtatacggc
      181 aacttgtgcc gtcacccacc cgaggcagga gaagctatgg ggcgggcggc ggaggttgtg
      241 gcaccttcat cgtccatacg aaccgatgga ggttgccccg tggtccaagt caatgatctc
      301 agccgcagcc tggccgcgtt tgatccgatc tcgaccttgg tggtggtcgt cgagatgagc
      361 aaggcgagct ggctggtgag cagcgtagtt cctggcgtgg agcgccagcc gttgaagaag
      421 ctggagccgg atgcgacggc agtgttgcga ttgatcgagc ggtggcggaa cgaggcggtg
      481 cgtgttggcc ggccgatcag ccggattgcg ctggcctatg aagctggtcg cgacggcttc
      541 tggctggcgc gctggctcat tgctcggggg atcgaggtgc atgtcatcca ttcggcaagc
      601 gttgcggtgt cacgcgagcg caagcgggcg aagacggatc gtctggatgc ggcgatgttg
      661 atgcgcgtat tcctgggctg gttgcgcggt gagcgggggc actgcgggat ggtggcgatc
      721 ccgacgatgg aggaggagga tgcgaggcgc ccgaaccgcg agcgcgagag ccttgtcaag
      781 gagcgttcac gcatcatcaa ccgcatgaag agtgcgcttg ctcgtctcgg gatccgggga
      841 ttcaagccac acctgcgcaa ggcgccagag cgtctggcag gcctgcggac agcggagggc
      901 acgggcctgc cggcaaacat catcgaggag ttccggcgcg acatggcacg gctggcattg
      961 gtccgtgagc agatcagctc gatcgagaag acacgtgccg agcggctcga gcgggcaccg
     1021 gatacagggc cgcatgcgat ggtgcggctg ctcgccaggg tgatcggtat cggtatcgag
     1081 acggcggaca tgctggtgcg ggagatcttg tcgcggaagc ttcgagatcg aagagcgctg
     1141 gcgcgctacg ccgggctcac gggatcgccc gacgagagcg gtttgaaaag ccgagagaag
     1201 ggcctggcca aggccggcaa tgcgcgggta cgacgtgggc tgatccagtt ggcatggcgc
     1261 ttcctgatgt tccagaaaga cagtgcgctg gcgcggtggt atcgcacgcg gaccgagggg
     1321 ccgagcggcg cgcgcaagac gacaatgatc gtggcactgg cgcgcaagct gctgatagcg
     1381 ctgtggcgcc tggtcacgac cggtgaggtg ccggacggtg tcgagttgcg gacggcggcg
     1441 tgagcaatac tgtagaacga gaaaggacaa acagagttcg gcacctgact gcacttgatg
     1501 cgcggtagcc gatgacgatc cgaggtggcg gcaatccgca tacggctatg gctgcaatgc
     1561 cgctgggaga atggacccgc cgcctcggag ccttgcccgc agaagcgtat gactgcttca
     1621 tggtcggggc cctatatggg ttcaaccgaa tacaagcgtg gttcgcgcgc gattgctcgt
     1681 ggccaatggc aggctcccct cggatcatcg cgtggcgaac cacccgatca cgggcagcct
     1741 gccgccgtgc tcgcgttggt taaggacaag ccttcggcgg cactgaaaaa gcgccgtcct
     1801 tgaccatcgc tgcgcgcgac ggcgatggta atatgcggtc gcgacggaag aatgctcgcg
     1861 gcgcgggtcg aacaaaagaa tgaagcaaca gcacgacaaa aagtggcgtg atgcaccctt
     1921 gacaaggaaa gccccataca agccgtatgc gggaaagccg cacgtacgga tctgggcggg
     1981 gggcgtgcca tgaaacgcac gtccctaccg ctacagaggc gcgaattcat cacgctgctc
     2041 ggcggtgcga ccgcgtgatc gctggcggcg caagcgcagc aatctgataa aatctggcgg
     2101 atgggtttta tcgcgcgcgg acaccaaaca ttttatgact cccttttcga ggcctccggg
     2161 aattggggta cgcg
//
LOCUS       DATIFQ010000058        19675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1934673, whole genome shotgun sequence.
ACCESSION   DATIFQ010000058 DATIFQ010000000
VERSION     DATIFQ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19675
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934673"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="VLE22_02445"
     CDS             complement(<1..1650)
                     /locus_tag="VLE22_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HSB13287.1"
                     /translation="MRPLDWVVLAVSLVFIVSYGLWRGRGSTSVRQYLLAGKTMPWYA
                     MALSIMATQASAITFISTTGQSYVDGMRFVQFYFGLPLAMVILSATAVPIFHRANVYT
                     AYEYLEKRFDAKTRALVSVIFLIQRGLAVGLALYAPAVVLTVIFGWPDRWTTMVMGLL
                     VVVYTTLGGIKAVTWTDFQQMLIMISGVIAALVAAVWLLPDGVSFLDAVTLAGAAGRL
                     NAVTFNVDWNDRYNLWSGLIGGMFLALAYFGCDQSQVQRYLTGKSIAQSRLSLLFNAA
                     AKVPVQFFILFTGAMVFVFFLFVKPPMLFQPVVLERIQAPSFQSDYVPLAERYDSAFE
                     NRRDAAMRLLEANRGGDADRRQAGVRDYREAQKRFDSVRREGAGLAEKAAGERGFNDT
                     NYIFLTFVTRYLPAGVVGLILAAIFAAAMSTISAEINSLATVSVIDIYQRHFRKDAAD
                     RHYLWTSRAATVFWGGYAVVTAEFGKHIGSLIEAVNMLGSLFYGGMLGVFALAFFFPR
                     VGAAGAFYGVIAGEAAIFAARFLTGISFLWYNVIGCLVVIAT"
     gene            complement(1647..1796)
                     /locus_tag="VLE22_02450"
     CDS             complement(1647..1796)
                     /locus_tag="VLE22_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13288.1"
                     /translation="MSNDQPAAKTVPDEPPPFLRTWSRVYAAIIGWLAVLIFLFFLFS
                     KAFSA"
     gene            complement(1793..4315)
                     /locus_tag="VLE22_02455"
     CDS             complement(1793..4315)
                     /locus_tag="VLE22_02455"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSB13289.1"
                     /translation="MGNRHRRTLFLLCLCLLSPPPYRAFAQRSLAGAVQTRLAIERLN
                     VLGSVLMIAAHPDDENTALLAYFARGRKFRTAYLSLTRGEGGQNLIGPEQGDLLGIIR
                     TQELLAARRIDGAEQLFTRAIDFGFSKSAEETLAKWGHEKVLADVVWNIRRFRPDVIL
                     LVFSGTSRDGHGHHQSSAILAKEAFAAAPDRNRFPEQLKWVEPWQVTRVFWNVFYWSA
                     ERERVIKEYPDHVRIDPGEFDPVLGYSYAEIAGMSRSMHRSQGFGAAERKGRSENYLV
                     VVAGEPAKTDPFDGIDTSWNRVPGGAEIGAILEEAARTFRPERPEETVPLLLKARPLV
                     AAVEHPWAARKLAELDETIALCTGLWLDASAERYAATPGSSLKVETVALNRSRFPLKL
                     AGVAVEGADGATGATAASMPLDYNQPVRQTITISLPPDQPYSQPFWLREPKDGNLYHI
                     GSPELVGVPDSPPALRARFRLVAGSQEIELVRPVAHRYVDRVMGEQVRPLDIVPPVGV
                     RIAEPVRMFPDNHARPLDVRLKANGSEVSGDLLLRIPSGWKASPGRIPFRMSGRGEER
                     VLNFDVTPPAGASRGEVTAIAVVGGREISSGAEVIEYPHIPPQAVFPPSRAELVRTDV
                     RVLARRIGYVVGAGDEVPQALRQLGCEVTLLSEEELVRGDLAQYDAIVTGVRAYNVRQ
                     DLRASQQRLMDYVQGGGTLVVQYNVLPFGGEAPALGKLGPYSLTVGRGRVSVEEAPVT
                     FPNPDHPLLSAPNHITAVDFEGWVQERGLYFASEWDQRYETLFESHDPGEAPLTGGTL
                     VARYGKGVYIFTAFSWFRQLPAGVPGAFRIFANFLSAGRSLR"
     gene            complement(4359..4835)
                     /gene="bfr"
                     /locus_tag="VLE22_02460"
     CDS             complement(4359..4835)
                     /gene="bfr"
                     /locus_tag="VLE22_02460"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HSB13290.1"
                     /translation="MRGNEKVIAVLNEMLKEELTAINQYFVHAEMCENWGYKKLSGYI
                     KKQSIGEMKHAESLLERILFLDGTPQMAQPLALKIGDSVKAQLANDLALEVSAVAAYN
                     KAVKLATEQGDNGSRDLLVRLLKDEEEHVDFLEAQLNLIKEVGYQNYLALQMGEGE"
     gene            4977..6005
                     /locus_tag="VLE22_02465"
     CDS             4977..6005
                     /locus_tag="VLE22_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB13291.1"
                     /translation="MATGTQAGMAPSPALVFDTLVAYQRSAALRAAIELDLFRAIGEG
                     PGDVASLARRCSASERGIRILCDYLVVIELLAKENGLYRHTPTSRVFLDPSSPACVAS
                     TALFLGNPTMYEPYLRLADIVRNGRTVLPGEGTVEPENPVWVEFAHSMAPMMAPMAAP
                     LGEIALDGLSGAVRVLDIAAGHGLFGIEVAKQRPGAQIVALDWAPVLEVARENARKAG
                     VQDGYTTLAGSAFDVEYGGPYDVVLLTNFLHHFDPPTCVGLLKKVHVSLKPGGRAATL
                     EFVPNEDRVSPPMAAAFSMVMLASTASGDAYTFRELEEMHRKAGFERISAHPIPNSPH
                     TVVIGHAR"
     gene            complement(6088..9300)
                     /locus_tag="VLE22_02470"
     CDS             complement(6088..9300)
                     /locus_tag="VLE22_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB13292.1"
                     /translation="MGTRFRFLVFPAMLAVAFLLVSRGSEDSRAKEERLWQHRNLGKA
                     FYENQTTRDEAVDEFRKALELAPDSLLDRVNYGLALLRTRKVEEGVAELERVQKADPS
                     IPHTWFNLGVAYKKNGDYPRAIAQFHRMAELVPGEPITHFNLGVLYRYTGKTEDALKE
                     FEIAAKLDPNFAAPLFQIFDTFREAERMQEAGAALKRFQLLKQRQDPDADAEDTNWSY
                     YSEIYEIVDPKDAAREASPPAELKFDGRKLAGTVDAQTAGMIAPDVDGDGLPELLAWS
                     SAGIRMYRNGLPLQQDALASLKDVVSVAAGDSNNDGLPELAVLTGSGPLLFLNEKGTY
                     RKLDVPLPPGRFEKAVWLDFDHDHDADLFLLGERCALLRNIGSGGFDDRTAAFPFVAG
                     HAIDAVAFRLVADTKAIDLLVSYKDRPSVLYKDRLLGNFEAQTLDQLPAGAGSLSSLD
                     VDNDGSIDVVFRLGKSLGVLRNRLGKLERAETGIAVPSGYAFADLETRGLADVIAGQA
                     VYRNQGMLKFAGAAKPAGLPDAVAWLQAEFDCDGRPDLAAVASDGSVLQFMNQTSTEN
                     NWVAVTLTGVKNPKLAPGTEVEVKAGTNYQKKVYTGLPLSFGLRNFQQVDAVRITWPN
                     GMIQNETRQPANRMLKFQEAPKLSGSCPMIFTWNGQRFEFITDVLGVAPLGASAGNGQ
                     FFAVDHDEYVQIPGRLLVPRDDRYEVRITEELSEVSYLDQARLIAVDHPSNVEIFGND
                     KWKSPPFPEFRLYGVNHRVYPRRARNHRGRDVLDRLVRRDLRYVDGFARDYEGVAEQH
                     WVELDFGDAARSNRAVLVLNGWVDWADGSTFLKVSQQKSGELQPPYLQVKDERGEWRT
                     VIDNMGMPSGKTKTLAVDLTGKFLSKSREVRIVTSMCVYWDEIFLGEDSSAPEARLTA
                     MDASNADLRFRGFSKVLIHPERKQPERFVYDKVRPVSTWNPTPGWYTRYGDVRELMRV
                     VDDRLVVMGPGDELRLSYDAARLPSLPEGWTRDFLLLVDGWEKDRDANTAYSQTVEPL
                     PFHGMTRYPYGLGERHPDEAYRTQWNTRPALRLIPPLAGD"
     gene            complement(9367..12039)
                     /locus_tag="VLE22_02475"
     CDS             complement(9367..12039)
                     /locus_tag="VLE22_02475"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HSB13293.1"
                     /translation="MSSDSATRPSRRQFLGAGVSAPAILGLPAVAEPAAVSPAAPAPA
                     TVDWKARWIWFPERRTLPCTFVFFRKEFELAQAPAKATGWVSGNSRYMLYINGRFVQR
                     GPAPCDPRYWDVDPVDLAPHLRPGQNVIGGLVCSFGGGDGTYVPGTPVGNPACAGFLF
                     QAEIPTAGGALELLTGDSWQSHRARSWPAGNYQRWYLRALQEEFDAREYPYGWNEAGF
                     DASGWRPAAVRDIPPGRPLLTELSPEGFHDDWRIEPRTIPLMTERVVHPERLTASGWV
                     DWQVPAGEYFDCFPPDAFTEQETPSVVLNRSSAGPFPLRLAAPGKRSAVVTFDFEGEV
                     AGYPFVRFRAPAGSVLEMLFLESQEPGRLLLRTNPNYGQWIRVTAREGETYYEAFDWD
                     VVRHLQLAVRGCEAPVEILECGVTQRLYPYPQQPDVTTSDEAINRAIRGAITTHLITS
                     QDTLMDNITRERQQYAGDVDHAKLVSYCAFGEYRQPRRMLETFAQGQNDEGWFMDSWP
                     AWDRCARLWQKSLKLTKWGPILDHGLGFVISAANYYLYSGDKTAIESLYPRFLRFDQF
                     LASNVGRDGLLPVTEWVRNSVWIDHRGFLAEIDKQASLNIYYIGYLLEGIARMADWLG
                     DSKHAALARRRASEVAETVRRHFWSAEHGLFVDNLPRVRKDGELRLHERTLAMALLYG
                     LFAPDQERAAVNVLADLPTGSNGPVFDLKRPRSKAGFSFPANGGWRLWALSRYGRADA
                     VVRDLRERWAAMVSLTENGTFSEDWEPRRNSVWCQNGPVILYVLYGDILGVQPTAPGF
                     RAFDVRPQPGGLDSVAGTIHSPAGPIRVDCTRGGGRTTVSLSTPAGVEPSLVTAAGAR
                     VDGLPVNARREPGPIAGTVRWKLPPLEKQWLFAF"
     gene            complement(12047..13363)
                     /locus_tag="VLE22_02480"
     CDS             complement(12047..13363)
                     /locus_tag="VLE22_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB13294.1"
                     /translation="MLDQPKKHRTIRHLRWWIGGLLFASTVINYIDRQTLSVMAPFLK
                     KEFTWTNSDFALIVISFRVAYAFGQTLSGRFLDWLGSRRGLMISVTWYSLVAMLVSTA
                     SGLKSFSFFRFLSGSGESANWPGAAKTVAEWFPKSERGWAVALFDSGSSVGGAIAPFL
                     VIWLYTTFGSWRPAFIFVGILGLIWVLIWRAFYHPPESHPNLSAEERDMILKSKREEQ
                     ETETRALRQDGDAGQRPASWRSLLGLKQTWGIILGRSLTDPVWFFITDWFAIYLVSKG
                     FKLENTLIGFWVPFLAADLGNFFGGGFSSYLIQRGWPVLRARKFVILICGIGMALLIP
                     VVFVSKFSLIITLFAISTFAYAAWSTMALSLPSDLYPSSTVASVSGMSGTGAGIGTII
                     STFLIGWVSDRYSFEPILVVASFVPLVATLLVFILVRDSKKQPAVA"
     gene            13449..14480
                     /locus_tag="VLE22_02485"
     CDS             13449..14480
                     /locus_tag="VLE22_02485"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSB13295.1"
                     /translation="MLRRDFLAAMTAFLKSDKVEEAARLIERATESGEVSAAVLRVQH
                     GGDVFSRAFGKAKTPETVFLIASITKPMTATGVMVLADRGMLSLSDPVRKFIPEFQGG
                     DRDSMTIRHLLTHTSGLPDMLPENIELRKRFSPLSEFVSGVVRTPLLFKPGARVQYQS
                     MGILLASEIARRVTDTAFPEFLRKEVFVPLGMNRTSLGLGSRKIPDTAICQVAEEKWG
                     WNSTYWRNLGAPWGGAHSTAVDINRFLRSFAKPDGKVLKPETARSMVTPQTKFPGESY
                     GFGWRTTRGGFGRACSERTYGHAGATGTLCWLDPDADLSFVVLTTKPLDASEKTLLHP
                     VSDLVSEAR"
     gene            14508..15563
                     /locus_tag="VLE22_02490"
     CDS             14508..15563
                     /locus_tag="VLE22_02490"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HSB13296.1"
                     /translation="MTDWRQHPHRRFNPLIREWVLVSPHRTQRPWQGQVERHPQPADQ
                     TYDPNCYLCPGNERARGARNPNYEHTFVFENDFAAMRPDTPFFESDQGGLMTVRSERG
                     VCRVVCFSPDHSLTLSRMTRPDIRRVVDVWAEQYFELAGIDWLNYVQIFENRGEMMGC
                     SNPHPHCQIWANEALPNEVSKEQEAQAEYRSANGSCLLCDYLTMEWKAGERLVCENDE
                     FAAFVPFWAVWPFETLVVSKAHATGLDELDPSQRDRLADILKRVTTRYDNLFETSFPN
                     TMGFHQRPTDGQAHQEWHLHIHFYPPLLRSATVRKFMVGYEMLASPQRDITAESAAER
                     LRGVGERHYLNDGAHEA"
     gene            15595..16662
                     /locus_tag="VLE22_02495"
     CDS             15595..16662
                     /locus_tag="VLE22_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB13297.1"
                     /translation="MERQFYLSLAARGLRMPIGTDLVLHEHDDPEAIMRNGRRLGQVM
                     ATAARRYRTPLAVPLMDLRLEKADLLRTLGVAESEVDTFHFSAAPGEESLAKVEAAMD
                     APFSQRNQANIDSIAYIAQETDLVPVGMAIGPFSLSTKLMADPITPIAMAGTGLTAEE
                     DSGVLMAERCLALAEMAVSRSIQAQVRAGAKAVIICEPAANIVYLSPKQIEAGSDIFE
                     RFVMGPDLRLKRRLSDVGADLIFHDCGELTAQMVREFANRLDPVILSFGSSRKLWEDA
                     ALVPKHVVLYGNLPTKSFYSDAAMPVEQVSELTNELTDKMHASGHPHILGSECDVLHV
                     PEAAATIRRKVEAMLDSTSLR"
     gene            complement(16666..17856)
                     /locus_tag="VLE22_02500"
     CDS             complement(16666..17856)
                     /locus_tag="VLE22_02500"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HSB13298.1"
                     /translation="MNRRAFLSSLAASPAVVAQTSRRYDILIRNGEVIDPARKFRRRA
                     DVAVLDGKIAAVEERIPEDRGLEVIDATGLYVSPGLVDLHTHCYHAATGLSIEADPIA
                     WRTGVTTWVDAGSFGSDQADGFRRFVVAPQHSRVFGYIHLYPNLRNPDVDVVKYVSSG
                     MKRTGEVAVANRDIILGVKVYVGANMNGRYSLDFLKIGRELGDTFKVPLMVHISFAPP
                     ETPEVMELLRAGDVVTHCFNTHTLGIMDREGRLKPGVKEARERGVLFDVGHGLGSFNF
                     DIGRKALDAGFPPDTISTDLYNLNTGGPVYDMPTTLSKFLYLGMSLDDVLLRATAAPA
                     KVIGRLPGMGTLEPGAPADIALLAMEEGEFRLVDSQKNAVVAKKRLVSRLTICRGRRM
                     VAPV"
     gene            complement(17873..19150)
                     /locus_tag="VLE22_02505"
     CDS             complement(17873..19150)
                     /locus_tag="VLE22_02505"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSB13299.1"
                     /translation="MARLTIISLLLLPALTYPQSEADKRLKEAGVCARCHVISVLEWG
                     YSRHPNIGVGCVECHGASKGHVIDERNNVKPDRIPRGAEVAALCKGCHQNPCPKTKRT
                     DSCQSCHHPHALIDPAKPPAPADKGVAEAAARSDRYALRMREGEQYAAAGKWEPARAA
                     FHSALGEAPGDRTAQRRLRMCERRLAPLLPGFEIVGSEFDAETGLPRAVRLTGFDVEM
                     VLVPGGDVEIGSDQFSGAKPVHTVRVEPFYLARTEVTQALWKSVTGANPSAHQGKDFP
                     DADRLPVEQISWQDAQAFFAKLSEKVEGGGLRLPTEAEWESAARAGGGSDEVLGAVKP
                     RPVGSGRPDRLGLFDMRGNVREWCSSLARPYPYDAGDGRESASEDGLRILRGSAYIEP
                     AGWFDATARHGERPGRRLRWNGLRLARAVPEAK"
     gene            complement(19138..>19675)
                     /locus_tag="VLE22_02510"
     CDS             complement(19138..>19675)
                     /locus_tag="VLE22_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13300.1"
                     /translation="RTMTMALDELEGIDYLFFPYNFIHARADYSQFLPAAIRQGVGLI
                     AMKPLAQGSIVQLDPRSPRGAAPESDRIQLWQRNNKPIIPAVVAELTKSLDRLPDETL
                     CMAALRFVFAKPFISTAIPGLFDMQWLEEDYRALARYHDMLPEEKAALDAARRLADLC
                     GPGWLPLHYRWLEEQWRA"
BASE COUNT         3828 a         6046 c         6327 g         3474 t
ORIGIN      
        1 ggtggcgatg accacgaggc agccgatgac gttgtaccag aggaacgaga tgccggtcag
       61 gaatcgcgcg gcgaagatcg ccgcttcacc cgcgatcacc ccgtagaacg cgccggcagc
      121 ccccacccgc ggaaagaaga acgccagcgc gaacactccg agcatgccgc catagaacag
      181 cgaaccaagc atgttcacgg cttcaatgag ggagccaatg tgcttgccga actcggcagt
      241 caccaccgca tagcctcccc agaacaccgt cgccgcacgc gaagtccaga gatagtgccg
      301 gtccgccgcg tctttccgga aatgccgctg gtagatgtcg atcacactca ccgtggccag
      361 ggagttgatc tcggccgaga tcgtcgacat cgccgccgca aagatcgcgg ccaggatcag
      421 cccgaccacg ccggcgggca agtagcgcgt cacgaacgtc aggaaaatgt agttcgtgtc
      481 gttgaatcct cgctcaccgg cggctttctc ggccagcccg gcgccttccc gcctgacgga
      541 atcgaaccgc ttctgcgcct ccctgtaatc cctaacgccg gcctgccgcc gatccgcgtc
      601 gccgccacgg ttcgcctcca ggagccgcat cgccgcgtcc cggcggtttt caaacgcgga
      661 gtcataccgc tcggcgagcg gcacgtagtc agactggaag gaaggggcct ggatcctctc
      721 cagcacgacg ggctggaaca acatcggggg cttgacgaac aggaagaaaa cgaacaccat
      781 cgcacccgtg aacaggatga agaactgcac gggcactttg gcggcggcgt tgaacagaag
      841 gctcagacgg ctttgcgcaa tcgacttacc cgtaaggtac ctctgcacct gcgactggtc
      901 gcatccgaaa taggcgaggg cgaggaacat cccgccaatc agcccgctcc ataagttgta
      961 gcgatcgttc cagtccacgt tgaacgtgac cgcgttcaga cgccctgccg cgcccgcgag
     1021 cgtcaccgca tcgagaaacg acacgccgtc gggcagcagc cagacggcgg cgaccagcgc
     1081 cgcgatcaca ccggagatca tgatcagcat ctgctggaag tcggtccagg tgacggcttt
     1141 gatgccgcca agcgtcgtgt aaacgacgac aagcaacccc atcaccatcg tcgtccaccg
     1201 gtccggccat ccgaaaatca cagtgaggac cacggcaggg gcgtacagag ccagccccac
     1261 ggccagcccg cgctggatca ggaaaatcac gctgaccagg gcgcgggtct tcgcgtcgaa
     1321 gcgcttttcc aggtactcgt aggcggtgta gacgttcgcc cggtggaaga tcgggaccgc
     1381 cgtggccgaa aggatcacca tcgcgagcgg cagcccgaag tagaactgca cgaagcgcat
     1441 gccgtccacg taggactggc cggttgtcga gataaacgtg atcgcgctcg cctgggtcgc
     1501 catgatcgag agcgccatgg cgtaccaggg catcgtcttg ccggcgagca ggtactgccg
     1561 gacgctggtg cttccccgcc cccgccacaa accataggag acgataaaca cgagcgaaac
     1621 cgccagcacc acccagtcga gcggtctcat gcggaaaagg ccttcgaaaa gaggaagaac
     1681 agaaaaatca ggacggcaag ccagccgata atcgcggcat aaacgcgaga ccacgtgcga
     1741 aggaacggcg gcggttcgtc cggaactgtc ttcgcggcgg gctgatcgtt gctcaacgga
     1801 ggctcctgcc ggcgctgaga aagttggcaa aaatccggaa ggctcccggc accccggcgg
     1861 gtaactggcg gaaccaggaa aaggcggtga aaatgtagac gcctttccca tagcgggcaa
     1921 ccagcgtgcc gcccgtcaag ggagcctcac cgggatcgtg agattcgaac agcgtttcgt
     1981 aacgctggtc ccattccgag gcgaagtaca ggccgcgttc ctgcacccag ccctcgaagt
     2041 ccacggccgt gatgtgattg ggcgccgaga gcaacggatg atccggattg gggaacgtca
     2101 ccggcgcttc ttccaccgag acacgaccgc gtccgaccgt tagagaatac ggtccgagtt
     2161 tcccgagtgc gggcgcttcc ccgccgaacg gcaggacgtt atactgcacg accagcgtgc
     2221 caccgccctg gacgtagtcc atgagccgtt gctggctggc gcgcaggtcc tgccggacat
     2281 tgtacgcgcg cacgccggtg acgatcgcgt catactgtgc gagatcgccc cgcaccagtt
     2341 cttcctcgct gagcagcgtc acctcgcatc cgagctgccg caacgcctgc ggcacttcat
     2401 cgccggcgcc gacgacgtaa ccgatccggc gcgcgagaac gcggacgtcc gtgcgcacga
     2461 gctccgcgcg cgagggcgga aagaccgcct ggggcgggat gtgcggatac tcgatcactt
     2521 cagcgccgga ggagatctcc cggccgccga caacggcaat cgccgtaact tccccgcggg
     2581 aagccccggc cggaggcgtg acgtcgaaat tgagcaccct ttcctcaccg cgcccggaca
     2641 tgcggaacgg gatacgcccc ggagacgcct tccagccgga tgggatccga agcagaagat
     2701 cgccggatac ttcggacccg ttggccttga ggcgaacgtc cagaggccgg gcatggttgt
     2761 ccgggaacat ccgcaccggc tcggcgatcc tcacacccac aggcggcacg atgtcgagcg
     2821 gccggacctg ttcgcccatc acacgatcca catagcggtg agccacgggc cgcaccagtt
     2881 cgatctcctg cgagccggca acgagacgga accgcgcccg cagggccggc ggcgagtcgg
     2941 gcacgcccac gagttccggt gagccgatgt ggtagaggtt gccgtccttc ggctccctga
     3001 gccagaacgg ctgcgaatac ggctggtcag gcggcagact gatggttatc gtttgacgaa
     3061 ccggctggtt gtagtccaga ggcatcgagg cggcagttgc acccgtcgcc ccatctgcac
     3121 cctccacggc gacccccgcg agcttgagcg gaaagcggga gcggttcagc gcgactgtct
     3181 ccaccttgag tgaagagccg ggggtggcgg cgtagcgctc ggcggaagcg tccagccaca
     3241 gtccggtgca aagagcgatc gtttcatcca gctcagccag ttttcgcgcc gcccaggggt
     3301 gttcgactgc cgctactagc gggcgcgctt tcaagagcag cggtaccgtc tcttcaggcc
     3361 gctccgggcg gaaagtgcgg gccgcctcct ccaggatcgc tccgatttcc gcgccaccgg
     3421 gaacgcgatt ccaggacgta tcgattccgt cgaacggatc cgtcttcgcc ggttcgcccg
     3481 cgaccaccac cagataattc tcagatcggc ctttgcgttc cgccgcgccg aaaccctggc
     3541 tccggtgcat gctacggctc ataccggcga tctcggcgta ggaatacccc agcaccggat
     3601 cgaactcgcc ggggtcgatt cggacgtggt ccgggtattc tttgatcacc cgctcgcgct
     3661 ccgcggacca gtagaaaacg ttccagaaca cccgcgtgac ctgccatggc tcgacccact
     3721 tcagttgttc ggggaatcgg ttgcggtcgg gggccgccgc aaaggcctcc ttggcgagga
     3781 tggccgagct ttggtgatgt ccgtgtccgt ccctggaagt tccactgaag acgagcagaa
     3841 taacgtccgg gcggaagcgg cgaatgttcc aaacgacgtc ggcaagcact ttctcgtggc
     3901 cccacttggc gagggtctcc tcggcggatt tcgagaagcc gaaatcgatg gcgcgggtga
     3961 agagttgctc ggcgccgtcg atgcgccgag ccgcgagcaa ctcctgcgtg cggatgatgc
     4021 cgagcaagtc gccctgctcg ggtccgatca ggttttgccc gccctcgcca cgagtcagag
     4081 agaggtaagc agtgcggaac ttccggccgc gagcgaagta ggcaagcaga gcggtgttct
     4141 catcgtcggg gtgagcagcg atcatcagca cgctgccgag aacattcaac cgttcgatcg
     4201 ccaggcgggt ctggacagct ccggccaggc tgcgctgcgc aaaagctcga tatggaggag
     4261 gactgagcag gcaaaggcac aggaggaata aagttctcct gtgccgattg cccatggaaa
     4321 ttctgcggct ggcaggcgtc tggcggccgc ggagcagctt actcgccctc ccccatctgg
     4381 agagcaagat agttctggta acccacctct ttgatgaggt taagctgggc ctcgaggaaa
     4441 tcgacgtgct cttcctcgtc cttcaggagc cgaacgagca ggtcgcggga gccattgtct
     4501 ccctgttccg tagccagctt cactgctttg ttgtaagcag ccacggcgga tacctccagg
     4561 gcgaggtcgt tcgcaagctg ggcttttacg ctgtcgccga tcttcagcgc aagcggctga
     4621 gccatctgcg gagttccgtc gagaaacagt atgcgctcca gaagggactc agcatgcttc
     4681 atctcgccga tggactgctt cttgatgtag ccactcagtt tcttgtagcc ccagttctcg
     4741 cacatctcag catggacaaa gtactggttg atggcggtga gctcttcttt cagcatttca
     4801 ttcaggacag cgatcacttt ttcattgcca cgcatgtagg tttgtctcct tcccagaatt
     4861 tcaattgtat caccggaaga aatacggcca gcgcgatcga acagcgggtc aaggtagact
     4921 tagattagtg tcgtctggga acgtatcgac actttactga aactgggagg tttcaaatgg
     4981 caactgggac tcaagcgggg atggcaccgt ctcccgcgct cgtcttcgac acactggtgg
     5041 cgtaccagcg ctccgcggcg ttgcgcgcag cgatcgaatt ggacctgttt cgggcgattg
     5101 gcgaagggcc gggtgatgtc gcctcactcg ccaggcgatg ctcggcctcc gagcgaggta
     5161 ttcgcattct gtgcgattac ctggtggtca tcgaactgct cgccaaggag aacggcctgt
     5221 accggcatac cccgaccagc agagtgtttc tcgacccgag ttcgccggct tgtgtggcat
     5281 cgactgcgct tttcctcgga aatccgacga tgtacgagcc ttaccttcgc cttgcggaca
     5341 tcgtgcggaa tgggcggacg gtcctcccgg gggagggtac ggtcgaaccg gagaaccccg
     5401 tctgggtgga gttcgcgcac agcatggcgc cgatgatggc gcccatggcc gctccactcg
     5461 gagagatcgc tttggatggg ctgtccggag cggtgcgcgt cctcgacatc gccgcggggc
     5521 acggactgtt cggcatcgaa gtagcaaagc aacgtccggg agcccagatt gtcgcgctcg
     5581 attgggcgcc ggtactggaa gtcgcgagag agaatgcgcg gaaggcgggc gtgcaggatg
     5641 gttacacaac gttggcgggc agcgcattcg acgtggagta cggcggcccg tacgacgtcg
     5701 ttctgctcac caactttctg caccacttcg atccacccac ctgcgtcggc ctgctcaaga
     5761 aagtacacgt atcgctgaag ccgggcggac gcgcggcaac gctggagttc gtgccgaatg
     5821 aagaccgcgt atccccgccc atggcagcgg ctttcagcat ggtcatgctt gccagcacag
     5881 cctccggcga cgcgtataca tttcgggaat tggaagagat gcaccgtaag gcagggtttg
     5941 agcggatcag tgcacatccc atcccgaaca gcccgcacac ggtggtgatc ggacacgcaa
     6001 ggtgagcggt agcccgttgt tcgctccctg acggccgcgg ttccaaagac tcgtggtgga
     6061 ccggcggtgg gagcaatgag cgaatcgtca gtcgccggcg agcgggggga tgaggcgcag
     6121 ggccggacgg gtgttccact gcgtccggta ggcttcgtcc ggatggcgct ctcccaaccc
     6181 gtacggatag cgggtcatgc cgtggaacgg cagcggctcc accgtctgtg aataagcggt
     6241 gttggcgtca cgatccttct cccagccgtc caccagcaag aggaagtctc gcgtccaacc
     6301 ctccggcaag gacggcagcc gcgccgcgtc gtagctgagc cgcaattcgt ccccgggacc
     6361 catgacaacc agcctgtcgt ccaccacgcg catcagttcc cgcacgtctc cgtaccgcgt
     6421 gtaccatccc ggcgttgggt tccacgtcga aaccggacgc accttgtcgt agacaaaacg
     6481 ctccggctgt ttgcgttccg gatgaatcag gaccttggag aagccgcgga accgcagatc
     6541 agcgttcgag gcgtccatgg cggtgagccg ggcctccggc gcgctcgaat cctctccgag
     6601 gaagatttcg tcccagtaca cgcacatgct ggtcacgatc ctcacttcgc gagacttcga
     6661 gaggaacttc ccggtcagat cgaccgcaag cgtctttgtt tttccggacg gcatccccat
     6721 gttgtcaatt accgtccgcc actcgccgcg ctcgtccttc acttgcagat atggcggctg
     6781 taattccccg ctcttctgct gcgacacttt gaggaacgtg ctgccgtcgg cccagtccac
     6841 ccatccgttt aacaccagga ctgcccggtt agagcgcgct gcgtcaccaa agtccaattc
     6901 cacccaatgc tgttcagcga ccccctcgta gtcccgcgcg aatccgtcaa cgtacctcag
     6961 gtcacgccgc acgagtcgat cgaggacatc gcggccccgg tgattccgtg cccgccgcgg
     7021 atagacccgg tgattcacgc cgtacagccg gaactcggga aacggcgggc tcttccactt
     7081 gtcgttgccg aagatctcga cgttcgacgg atggtccacg gcaatcagtc gcgcctgatc
     7141 gaggtaggac acctcgctca actcctcggt gatccgcact tcgtagcggt cgtcacgcgg
     7201 gaccagtaac cggcccggga tctggacgta ttcgtcgtga tccaccgcga agaactggcc
     7261 gttgcccgcg ctcgccccca gcggtgcgac gccgagcacg tcggtgatga attcgaagcg
     7321 ttgaccgttc caggtgaaga tcatcggaca agacccggac agcttcggtg cttcctggaa
     7381 cttcagcatg cggttggcag gctgcctggt ctcgttctgg atcatcccat tcggccacgt
     7441 gatgcggacg gcgtccacct gctgaaagtt gcgcaaaccg aaactgagcg gcagcccggt
     7501 gtaaaccttc ttctgatagt ttgtgcctgc cttgacttcg acttccgtgc ccggtgcgag
     7561 ctttgggttc ttgacacccg tgagagtgac cgcgacccag ttgttctccg tgctggtctg
     7621 attcatgaac tgaagcacgg aaccatccga ggcgaccgcc gccaggtccg gacggccatc
     7681 acagtcgaac tcggcttgaa gccaggcgac cgcgtccggc agtccggcag gcttcgccgc
     7741 gcccgcgaac tttagcatcc cctgattccg atagacggcc tgtcctgcga tcacgtcggc
     7801 cagcccgcga gtctccagat ccgcgaacgc ataaccgctt gggacggcga tcccggtttc
     7861 ggcccgctcc aacttgccca gcctgtttct gagcacgccg aggctcttcc cgaggcggaa
     7921 aacgacatcg atcgacccgt cattatccac gtcgaggcta gaaagcgaac cggcgcccgc
     7981 gggcaattga tcgagagttt gcgcctcgaa gttgccgaga agccggtctt tgtacaggac
     8041 agacggccgg tccttgtagg agacgaggag gtcgatggcc ttggtgtcgg ccacgagccg
     8101 gaacgccacc gcgtcgatgg catgaccggc gacaaacgga aaggcggcgg tgcggtcgtc
     8161 gaaaccgccg gacccgatat tgcggagcag cgcgcagcgc tcgcccagca ggaacaggtc
     8221 ggcgtcgtga tcgtgatcga agtcgagcca gaccgctttc tcgaaccggc cgggcggaag
     8281 aggcacatcc agcttccggt acgtgccttt ttcgttcagg aataacaacg ggccggagcc
     8341 ggtgagcacg gcgagttcgg gaagcccgtc gttattggag tcgcccgcag ccacgctcac
     8401 cacgtctttg agagaggcaa gcgcgtcctg ctgcagggga agaccgttcc ggtacatccg
     8461 gatgccggcg gatgaccatg ccagcaactc cggcagtccg tcgccatcca cgtcgggtgc
     8521 aatcatgccg gcagtctgcg cgtccaccgt acccgcgagc ttccggccgt cgaatttcaa
     8581 ttctgcaggc ggggaagctt ccctggcggc gtctttcgga tcgacgatct cgtagatctc
     8641 cgagtagtag ctccagttgg tgtcctcggc atcggcgtcg gggtcctggc gctgcttgag
     8701 caactggaac cgcttgagtg cggcgcccgc ttcctgcata cgctcggcct cccggaatgt
     8761 atcgaagatc tggaacagcg gcgcggcgaa gttcggatcg agcttcgcgg cgatctcaaa
     8821 ctccttcagg gcgtcttcgg tcttcccggt gtaacggtag agcacaccga ggttgaaatg
     8881 cgtgatcggc tcgccgggaa cgagctccgc catgcggtga aactgggcga tggcgcgcgg
     8941 gtagtcgccg ttcttcttgt aggcgacgcc caggttgaac caggtgtgcg ggatggacgg
     9001 atccgctttt tgcactcgtt cgagttcggc gacgccctcc tcaacctttc gggtgcgcag
     9061 caaggcgagt ccgtagttca ctcgatcaag gagagagtcg ggcgcgagtt cgagcgcttt
     9121 tcgaaactca tccacagcct catcgcgggt cgtttggttt tcatagaatg ccttgccgag
     9181 gttgcgatgt tgccacagac gctcttcctt cgcacgggaa tcctcggaac cgcgcgacac
     9241 caggaggaag gcgacagcca gcatcgccgg aaacacgaga aaccggaagc gagtgcccat
     9301 ggaaagggag tccttatctc tcgaccagta tagacagggc ggaggagtga ccgtatcagg
     9361 agcggctcag aaagcgaaaa gccactgttt ttccaaaggc ggcagcttcc agcggacggt
     9421 gccggcgatt gggccgggtt cgcggcgtgc attcaccggc aggccgtcta cccgggcgcc
     9481 cgcggccgtg accagcgacg gctcgacgcc ggccggagtc gaaagcgata cggttgtccg
     9541 gccgccgccg cgagtgcagt ccacgcgaat cggtccggcg ggcgagtgaa tcgtgccggc
     9601 tacggagtcg aggccgccgg gttgcggccg cacgtcgaag gcacggaatc cgggtgccgt
     9661 gggttgaacc ccgaggatgt cgccgtagag aacatagagg atgaccggac cgttctgaca
     9721 ccagacgctg ttccggcgcg gctcccagtc ctcgctgaag gtgccgttct cggtgagcga
     9781 taccatcgcc gcccagcgct cgcgaaggtc gcgcactacg gcgtccgcgc gcccgtagcg
     9841 gctgagcgcc cacaatcgcc aaccgccatt cgcggggaag gagaagccag ccttcgagcg
     9901 gggcctcttc aggtcgaaga ccggcccgtt ggaaccggtg ggcagatccg ctagcacgtt
     9961 caccgcggcg cgttcctggt ccggagcgaa aagtccgtag aggagggcca tggccagcgt
    10021 gcgttcgtgg aggcgcaact cgccgtcctt gcgcacgcga ggaagattgt ccacgaacaa
    10081 accgtgttcg gcggaccaga agtgccttcg taccgtctcc gccacttcgg acgcacggcg
    10141 gcgggccaga gcggcgtgtt ttgagtcgcc aagccagtcc gccatgcggg cgatgccttc
    10201 gagtagatag ccgatgtagt agatgttgag cgatgcctgc ttgtcgatct ccgcgaggaa
    10261 gcctcggtgg tcgatccata cactgttgcg gacccattcc gtcacgggaa ggagtccgtc
    10321 gcggccgaca ttggaggcca ggaattggtc aaagcgcaga aaacgagggt agaggctctc
    10381 gatcgcggtt ttgtcgccgg agtacaggta gtagttagcg gcggagatga caaagccgag
    10441 gccatggtcg agaatcggtc cccacttggt tagcttgaga ctcttctgcc aaaggcgcgc
    10501 gcaacggtcc cacgcgggcc aactgtccat gaaccacccc tcgtcgttct ggccctgcgc
    10561 gaacgtttcg agcatccgcc ggggctggcg gtactcgccg aacgcgcaat aggagacgag
    10621 cttggcatgg tccacatcac cggcgtactg ctgccgctcg cgggtgatgt tgtccatcag
    10681 agtgtcctgc gacgtgatga ggtgcgtggt gatggcgccg cggatggcgc ggttgatggc
    10741 ttcgtcggag gtggtgacgt ccggctgttg cgggtaagga tagagccgct gggtgacgcc
    10801 gcactcgaga atttcgaccg gggcttcgca gccgcggacg gcgagttgaa ggtgtcgaac
    10861 tacatcccaa tcgaaggcct cgtagtacgt ctctccttcc cgagcggtga cgcgaatcca
    10921 ctggccatag ttgggattgg tgcggaggag gagtctgccc ggctcctgcg attcgaggaa
    10981 cagcatctca agaacacttc cggcgggcgc acggaagcgg acgaagggat agccggccac
    11041 ttcgccttcg aagtcgaacg tcacgacggc cgagcgcttt cccggcgccg caaggcgaag
    11101 aggaaacggg ccggcagagg agcgattcag cacgactgaa ggggtttcct gttcggtgaa
    11161 ggcatcgggt ggaaagcagt cgaagtattc cccggcaggc acttgccaat cgacccaacc
    11221 ggaagcggtg agtctttcag gatggacaac gcgctcggtc atcaacggga tggtgcgggg
    11281 ttcgattcgc cagtcatcgt ggaaaccttc gggagacagc tccgtgagca agggccgtcc
    11341 gggaggaatg tcccggacgg cggcagggcg ccagccggag gcgtcgaaac cggcctcatt
    11401 ccagccgtac ggatactcgc gggcatcgaa ctcttcctga agggcccgca ggtaccagcg
    11461 ctgatagttg ccggcaggcc aggaccgggc acgatgcgac tgccaggagt cgccggtcag
    11521 cagctcgagc gcaccgccgg cggtggggat ctccgcctgg aagaggaagc ccgcacaagc
    11581 cggattgcca accggggttc cgggaacata cgtaccatcg cctccgccga acgaacacac
    11641 gaggccgccg atgacgttct ggcctggccg caggtgcgga gcgagatcga ccgggtcgac
    11701 gtcccaatat cgcgggtcgc agggagcggg gccgcgctgg acgaatcgcc cgttgatgta
    11761 aagcatgtag cggctgttcc cggataccca gcctgtcgcc ttggcgggcg cctgcgcgag
    11821 ttcgaactct ttgcggaaga aaacgaacgt acaggggagc gtgcgccgct ccgggaacca
    11881 gatccagcgg gccttccagt caaccgtcgc cggcgcgggc gcagcggggc tgacggcggc
    11941 cggttcagcg accgccggga gtccgaggat agcgggcgcg gagacaccgg ccccgagaaa
    12001 ctggcggcgg gatggccgcg tagctgaatc gctgctcatg gacgcctcac gcaaccgcgg
    12061 gctgtttttt actgtcgcgg accaggatga acaccagcaa agtcgccaca agcggcacga
    12121 agctggccac gacgaggatc ggctcgaacg agtagcggtc cgaaacccag ccgatcagga
    12181 acgtcgaaat gattgtgccg atgcccgcgc cggtgccgct catcccgctg acggaagcca
    12241 ccgtgctgct cggataaagg tccgacggca atgagagcgc catggtggac caggcggcat
    12301 aggcgaaggt cgagatcgcg aacagcgtga tgatcaggga gaatttcgaa acgaagacga
    12361 ccggaatcag aagcgccatg ccgataccgc agatgaggat gacgaacttt ctcgcgcgga
    12421 gcacaggcca gccccgctgg atcagatagc tcgagaagcc gccgccgaag aagttgccca
    12481 agtcggcagc caggaatggc acccaaaaac cgatgagcgt gttctcgagc ttgaagcctt
    12541 tggagactag gtagatcgcg aaccagtccg tgatgaagaa ccagaccgga tcggtgagcg
    12601 aacggccgag gatgataccc caggtctgtt tcaggccgag caagctgcgc caggaggcgg
    12661 gccgttggcc ggcgtcgccg tcttggcgaa gcgcgcgagt ttccgtctcc tgctcttccc
    12721 gtttcgactt gagaatcatg tcgcgctctt cggcgctgag attcgggtgt gattccggag
    12781 gatggtagaa cgcccgccag atcagtaccc agatcaagcc gaggataccc acgaagatga
    12841 acgcgggtcg ccagcttccg aacgtagtat agagccagat gacgagaaac ggcgcgatag
    12901 cgcctccaac cgaggaaccg ctgtcaaaca gcgcaaccgc ccatccgcgt tccgacttcg
    12961 ggaaccactc ggcgacggtc ttggcagctc ccggccagtt ggccgactcg ccggaaccgg
    13021 agaggaaccg gaagaatgag aagctcttca gtccggacgc cgtcgaaacc agcatcgcga
    13081 ccagcgaata ccaggtgacg gaaatcatca gcccgcgccg ggagccaagc caatcgagaa
    13141 aacggccgga gagcgtttgt ccaaaggcat aggcaacgcg gaagctgatc acgatgaggg
    13201 cgaaatcgga attcgtccag gtgaattcct tcttgaggaa cggcgccatc acggaaagtg
    13261 tctggcgatc gatgtagttg atcacagtgg atgcgaaaag tagtccaccg atccaccaac
    13321 ggagatggcg gatggtccta tgcttctttg gctgatcgag caaagtattg gctccctgga
    13381 ggcgctggat gtccgtggga cagaccctac atgatatcga agcgccgctt tccgggtata
    13441 gtcgaaagat gctccggagg gattttctag cggcgatgac ggcgtttctc aagagcgata
    13501 aggtcgaaga ggcggctcgc ttgatcgagc gggcgacgga gtcgggtgaa gtgtcggctg
    13561 cggttctacg cgtccagcac ggaggcgacg ttttctcccg ggctttcggc aaggcgaaga
    13621 cgcccgagac ggtatttctg atcgcatcga tcaccaagcc gatgacggcg acgggcgtga
    13681 tggtcctggc ggatcgcggg atgctctccc tctccgaccc ggtgcggaaa ttcattccgg
    13741 agtttcaagg cggggaccgc gactccatga cgattcgcca tctgctgacg cacacgtcgg
    13801 gattgccgga catgctgccg gagaatatcg aactgcgcaa gcgcttctcg ccgttgagcg
    13861 agttcgtgag cggcgtcgtt cggacgccgt tgctcttcaa accgggcgcc cgcgtacagt
    13921 atcagagcat gggtatcctg ctggcgtcgg agatcgcccg gcgagttacg gacaccgcat
    13981 ttccggagtt tctgaggaaa gaagtttttg tgcccctcgg tatgaatcgg acctccctcg
    14041 ggctcggcag ccgcaagatt cccgataccg cgatttgcca ggtggcggag gagaaatggg
    14101 gctggaacag cacgtactgg cggaatctcg gcgcaccatg gggaggggcc cattcgaccg
    14161 ccgtggacat caatcgcttt cttcgcagtt ttgcgaagcc ggatggaaaa gtgctgaaac
    14221 cggagaccgc taggtccatg gtgacgccgc agacgaagtt tccgggcgag tcgtacggct
    14281 tcggctggcg gacaactcgc ggtgggttcg ggcgtgcctg ctcagagcgg acatacggcc
    14341 atgccggggc caccggcacg ctgtgctggc tggatccgga tgcagacctg agttttgtgg
    14401 tgctcactac gaagccgttg gacgcatccg aaaagaccct gctgcatcct gtctccgacc
    14461 tggtttcgga ggcgcgctag aatcgtgcat tcggaggcaa tcccaagttg accgactgga
    14521 ggcagcatcc gcatcgccgc ttcaatccgt tgatccgcga atgggtcctc gtctcaccgc
    14581 accggacaca acggccgtgg caaggccagg ttgagagaca cccccagccc gcggaccaaa
    14641 cttacgatcc gaattgctac ctgtgcccgg gcaacgagcg cgcacggggg gcgaggaatc
    14701 cgaactacga gcatacgttt gtcttcgaga acgatttcgc cgccatgcgg ccggatacgc
    14761 cgttcttcga gtccgaccag ggcgggctga tgacggtacg cagcgagcgt ggcgtctgcc
    14821 gggtggtgtg cttctcaccg gatcacagcc tgacgctctc gcgcatgacg cgaccggaca
    14881 tccggcgggt cgttgatgtg tgggcggagc aatacttcga actcgcgggc atcgactggc
    14941 tgaactacgt gcagatcttc gaaaaccgcg gagagatgat gggatgcagt aaccctcatc
    15001 cgcactgcca gatctgggcg aatgaggcgc tgcccaacga ggtctccaag gagcaggagg
    15061 cgcaggccga atatcgaagc gcgaacggtt cctgcctgtt gtgcgactac ctcactatgg
    15121 agtggaaagc gggcgagcgc ctggtctgcg agaacgacga atttgcagcg ttcgtgccgt
    15181 tctgggccgt ctggccgttt gagacgctgg tcgtcagcaa ggcccatgca acgggactcg
    15241 acgaactcga cccttcgcaa cgggacaggc tggcggacat cctgaagcga gtgaccacgc
    15301 gctacgacaa cctgttcgag acatcgttcc cgaacacgat gggattccac cagcggccca
    15361 ccgacgggca ggcacatcag gagtggcact tgcacattca cttctacccg ccgcttctgc
    15421 gctcggcgac cgtccggaag ttcatggtgg gctatgagat gctggcgagc ccgcaacggg
    15481 acatcacggc ggagagcgcg gccgaacgat tgcgcggggt cggcgaacgg cactacctga
    15541 acgacggcgc tcacgaagcc tgaatgccgc aacggccgtg agatactggc cgggatggaa
    15601 cggcaattct acttgagcct ggcggcccgc ggcctgcgga tgccgatcgg gacggatctc
    15661 gttcttcacg aacacgatga tccggaggcg atcatgcgaa acgggaggcg gcttggtcag
    15721 gttatggcaa ccgccgcccg gcgctatcgg acgccgctcg ccgttccttt gatggatctt
    15781 cgcctcgaaa aggctgacct gttgcggacg ttgggcgtgg ccgagtcaga ggtggacact
    15841 ttccacttca gcgccgcacc gggggaggaa tcactcgcga aggtcgaagc ggccatggat
    15901 gcgccgttca gccagaggaa ccaggcgaac atcgactcga tcgcttacat cgcccaggaa
    15961 accgacctcg tgccggtcgg catggcaatc ggcccatttt ccctttccac caagctgatg
    16021 gcggacccga tcacgccgat cgccatggcc ggcacaggtt tgaccgccga ggaggatagc
    16081 ggtgtgctga tggcagagcg ctgcctggct ctcgccgaga tggccgtctc cagatcgatt
    16141 caggcccagg ttcgcgcagg tgcgaaggcg gtcattatct gtgagcccgc agcgaacatc
    16201 gtgtacctgt cgccgaagca gatagaggcc ggctcggaca ttttcgagcg gttcgtcatg
    16261 ggaccggact tgcgtctgaa gcggcgcctt tccgatgtgg gcgccgacct gatcttccac
    16321 gattgcggcg aactcaccgc gcaaatggtg cgggagttcg ccaaccggct cgatccggtg
    16381 attctcagtt tcggcagctc acggaagctg tgggaggacg cggcactcgt gccgaagcac
    16441 gtcgtgcttt acgggaatct tcccaccaag agcttttact ccgacgccgc catgccggtc
    16501 gaacaggtca gcgaactgac caacgagttg accgacaaga tgcacgccag cggacatccg
    16561 cacatcctcg ggtcggagtg cgacgtgctg catgtgccgg aggccgcggc gacgatccgc
    16621 cgaaaggtgg aagcgatgct ggactccact tcgctgcgat gaccgttaga ccggcgcgac
    16681 catccgccgg ccacggcaga tcgtcagacg gctgacaagg cgtttcttag cgaccacggc
    16741 attcttctgg gagtcgacga gccggaattc gccctcctcc atcgcgagca gcgcgatgtc
    16801 cgccggagcg cccggttcga gagtgcccat gccgggcagg cgaccgatca ccttcgcagg
    16861 ggcagcggtc gcgcggagca gcacgtcgtc caggctcatg ccaagataaa ggaacttcga
    16921 cagcgtggtc ggcatgtcat aaaccggccc gcccgtgttg aggttgtaca ggtcggtcga
    16981 gatcgtgtcc ggggggaagc cagcgtcaag cgccttccgg ccgatgtcga aattgaagct
    17041 gcctaggccg tggccgacgt cgaacagaac gccccgttcg cgagcttcct tgacgccggg
    17101 cttcagccgg ccttcgcgat ccatgatgcc aagcgtgtgg gtgttgaagc agtgggtgac
    17161 gacgtcgcct gctcgcaaca actccatcac ttcgggtgtt tcgggcggcg cgaagctgat
    17221 gtgaaccatc aggggaacct tgaaggtgtc gccaagctcg cggccgattt tcaggaaatc
    17281 gaggctgtag cgcccgttca tattcgcacc gacatagacc ttgacgccaa ggatgatgtc
    17341 gcgattggca accgcaactt cgccggtgcg tttcatgccg gagctgacat acttcactac
    17401 gtccacgtca ggattgcgca gattggggta gaggtggatg tagccgaaca cccgcgaatg
    17461 ctgcggcgcg acgacgaacc ggcgaaaccc gtcggcctga tctgagccga agctgccggc
    17521 atcaacccat gtggtaacgc ccgtccgcca agcaatcgga tcggcttcga tgctgaggcc
    17581 ggtcgcggcg tgataacagt gtgtgtggag atcgacgagg ccgggggaaa cgtacaaacc
    17641 ggtcgcatcg atcacttcca atcccctgtc ttccgggatc cgttcttcga cagcggcgat
    17701 cttgccgtcg aggacggcga cgtccgcgcg gcggcggaac ttgcgtgccg ggtcaatgac
    17761 ctcaccattc cggatcagga tgtcgtaacg gcgactcgtt tgcgccacaa cggccggaga
    17821 ggcggctagg ctggagagaa aggctcgtcg gttcatcaga agggtctcct gttcatttcg
    17881 cttcgggcac tgcgcgggcg agccgcaggc cgttccatcg caaacgccgt ccggggcgtt
    17941 ctccgtggcg ggcggtggcg tcgaaccagc cggcaggttc gatgtaggcg ctcccgcgca
    18001 gtatacgaag tccgtcttcc gaggccgact cgcggccgtc gccggcatca tacggatatg
    18061 gacgcgcgag actcgagcac cactcgcgaa cgtttcctcg catgtcaaac agaccgagac
    18121 ggtcgggccg gcccgaccca acgggccttg gcttcaccgc accaagcact tcgtctgagc
    18181 cgccaccggc gcgagcggcc gactcccatt cggcctcggt gggaagtcgg agcccgccgc
    18241 cctccacctt ttcactcagc ttcgcaaaga aggcctgggc atcctgccaa gaaatctgtt
    18301 cgacaggaag acggtcggcg tccgggaagt ccttgccctg gtgggcgctg ggatttgcgc
    18361 cggtcaccga tttccagagc gcctgcgtga cctcggttcg cgctagataa aaaggctcca
    18421 cgcggacggt atgcaccggc ttcgcgccgc tgaactgatc cgagccgatc tcgacgtcgc
    18481 caccgggcac gagcaccatc tcaacatcga agccggtcag ccggaccgcg cggggcaacc
    18541 cggtctccgc atcgaactca ctaccgacga tctcgaagcc aggaaggagg ggggcgagac
    18601 gccgctcaca catccgaagc cgccgctgtg ctgtccggtc gccaggtgct tcgccgagcg
    18661 cggagtgaaa ggctgcgcgg gccggttccc atttcccggc ggcagcatac tgttcaccct
    18721 cgcgcatccg gagcgcgtag cggtccgagc gggcggcggc ttccgcgacg cctttgtccg
    18781 cgggcgccgg aggcttcgcc ggatcgatca gcgcgtgagg gtggtgacag ctctggcaag
    18841 aatcggttcg cttcgtcttc gggcaggggt tctgatggca gcctttgcag agagcggcga
    18901 cctcggcgcc gcgggggata cggtccggct tgacgttgtt ccgctcgtcg atcacgtggc
    18961 ccttgctcgc tccgtgacac tcgacacaac ccacgccgat gttcgggtgc cgcgagtagc
    19021 cccattccag cacggagata acgtgacagc gagcacagac gccggcttcc ttgagtcttt
    19081 tgtcagcctc actctgcgga tacgtgaggg cgggcaagag cagcagggag attatcgtca
    19141 ggcgcgccat tgttcctcca gccagcggta gtggagcggc agccagccag gtccgcagag
    19201 gtccgcaagg cggcgcgcgg cgtcgagagc agccttctct tccggcagca tgtcgtggta
    19261 tctcgcgagc gcgcgatagt cctcctccag ccattgcatg tcgaacaagc ccgggatcgc
    19321 cgtcgagatg aagggcttcg cgaagacgaa acgcaacgca gccatgcaga gcgtctcgtc
    19381 ggggagacgg tcgaggctct tcgtcaattc tgcgacaact gcgggaatga tcggcttgtt
    19441 gttgcgctgc cagagctgta tacggtcgct ttcgggtgcg gctcctcgag gcgaacgggg
    19501 atcgagttgc acgatcgaac cctgcgccag cggtttcatc gcgatcagac cgacgccctg
    19561 gcggatcgcg gcgggcagga actggctgta gtcggcgcgt gcgtggatga agttgtatgg
    19621 gaaaaagagg tagtcgatgc cttcgagttc gtcgagggcc atcgtcatgg tgcgt
//
LOCUS       DATIFQ010000059         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2317277, whole genome shotgun sequence.
ACCESSION   DATIFQ010000059 DATIFQ010000000
VERSION     DATIFQ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317277"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VLE22_02515"
     CDS             complement(<1..797)
                     /locus_tag="VLE22_02515"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSB13301.1"
                     /translation="MTNNTPLAGRRALVTGASGFIGSALCRDLTQAGMEVHGVSRTER
                     AATDFCARWHSNRLDDIEAVRALFKAARPDFVFHLASHVAGSRSIELVLPTFSANLAS
                     TVNLLTAGTELGCERILLTGSLEEPEPGPDHAVPSSPYAAAKFAASAYGRMFHALYKT
                     PVAILRLFMVYGPGQQDLRKLVPYVTLSLLKGQTPELSSGARKVDWIYVDDVVAGYIA
                     AASAKEVEGSTIDLGSGRLDTVRTVVEELVGLINPRVQPLFGSIPERA"
     gene            898..1200
                     /locus_tag="VLE22_02520"
     CDS             898..1200
                     /locus_tag="VLE22_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13302.1"
                     /translation="MNTLKNGLRPVQYFMIPKLQNPVAQGFQIGCPLTISVLLFLMLT
                     AIVLNHQLDVRTHEIDDVRLNHQLVPEPETTETTHPQVMPQQAFCIRHLTSQHPGQ"
     gene            complement(1679..>2133)
                     /locus_tag="VLE22_02525"
     CDS             complement(1679..>2133)
                     /locus_tag="VLE22_02525"
                     /inference="COORDINATES: protein motif:HMM:NF027546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HSB13303.1"
                     /translation="YDERQYCSPGFNLPVGCLMRSPHGSFPEYHTSADNLDFVRPENL
                     ATSLDAYRHVVEVLEGNGSYLNLNPKCEPQLGRRGLYSAVGADPNQKLDQMALLWVLN
                     LSDGAHSLLDVCEMANLPFREIKRAADALERVGLLRKCAAEPPAPTGS"
BASE COUNT          434 a          684 c          587 g          428 t
ORIGIN      
        1 gcccgttccg gtatcgatcc gaataatggc tggacgcgcg gattgatcaa cccaaccaac
       61 tcctccacca ccgtccgtac ggtgtccagt ctgccgctcc ccaagtcaat cgtgctcccc
      121 tccacttctt tcgcagaggc tgcggcgatg tacccggcca cgacgtcgtc cacgtatatc
      181 cagtcgactt tccgcgcccc gctcgacaac tccggtgttt gtcccttcag cagggagagc
      241 gtcacgtagg gtaccagttt ccggaggtcc tgctggccgg ggccgtagac cataaagaga
      301 cgcaggatcg ctaccggtgt tttgtacagc gcgtgaaaca tccggccata ggcactggcg
      361 gcgaacttgg cggctgcata cggcgagctc ggcactgcat ggtctgggcc cggttcgggc
      421 tcttccaggg aacccgtcag caagatccgc tcgcaaccca gctcggtgcc ggcggtcagg
      481 agatttacgg tactggcgag attggcggag aacgtgggca ataccagctc aatcgaacgg
      541 gatcccgcga cgtggctggc gaggtgaaaa acgaagtccg gcctcgccgc cttgaacaga
      601 gcacgcactg cctcgatgtc atcaagccgg tttgaatgcc atcgcgcgca gaagtccgtg
      661 gcggcacgct ccgtccgcga caccccgtgg acctccatcc ccgcctgcgt caggtcgcga
      721 cataacgccg atccgatgaa tccgcttgcg ccggtaacaa gggcgcgacg tccagccaat
      781 ggggtgttat tggtcatagg tttgttcggc acttccgcag aatgcgtggg tcggaccgcc
      841 gtcagctgcc tctcgctccg ggagagggtt ggggtgaggg gatttaatca gttcgtgatg
      901 aatacgctca agaacggcct gcgtcccgtt cagtatttca tgattccaaa attgcagaac
      961 ccggtagccc agggtttcca gataggctgt cctctcacta tctcggtcct gctgttcctc
     1021 atgctgaccg ccatcgtcct caaccaccaa cttgacgtca ggacacacga aatcgacgat
     1081 gtacggctca atcaccaact ggtgccggaa cctgaaacca cagagacgac gcacccgcaa
     1141 gtgatgccac aacaagcgtt ctgcatccgt catttgactt cgcagcatcc gggccaatga
     1201 cttcatgtat ttccgatccc ctctccctgg ccccctgttc ccgcccaggg tgcacgagaa
     1261 tcgcgtgcac ccgttcgacg gggaaaactt cggcgctgcg ctttcctgct ccgcgcgaag
     1321 tccagcccgt tgccagcccg gggcaggcaa cagctgcccg cccgttcgac ggcgagaacg
     1381 ttcaccgaac gttctcgaaa cctccgtctc acccctggcc gggcgttcgc ggctcgcatt
     1441 ctcgccgctc acccgccgga ccttttcctg tttccgcctc accccggagg gagagggatt
     1501 aataattaac actttcacgg ccaaccggct ccaggatatt acagtgaccg actgcatagc
     1561 caaaatctgc gcggcatgaa ttcaagaaca tcttcacaac cacagatggt tacggtggta
     1621 aaaatgctca atctacccaa aatccagcga aagagtgttt attcttgtta ccacgccatt
     1681 agctgccggt cggcgcagga ggctccgcgg cgcatttccg gagcagcccg acacgctcca
     1741 gcgcatcggc cgcgcgcttg atctcccgga atggcagatt cgccatttca cagacatcca
     1801 gcaggctgtg cgcaccatcg gaaagattca acacccataa cagcgccatc tgatccagtt
     1861 tctggttcgg gtccgccccg acagcgctgt acaagcctct gcgtccgagc tgcggctcgc
     1921 atttgggatt gagattgagg tagcttccgt tgccttcaag gacctcgacg acatgacgat
     1981 aggcgtcgag cgacgttgcc aggttttccg ggcgcacgaa gtccaggttg tccgcggagg
     2041 tgtggtactc gggaaaactc ccatgcggcg agcgcatgag gcatcctacc ggcagattaa
     2101 acccgggaga gcaatattgc cgctcgtcat aac
//
LOCUS       DATIFQ010000060        19778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1551154, whole genome shotgun sequence.
ACCESSION   DATIFQ010000060 DATIFQ010000000
VERSION     DATIFQ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19778
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551154"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..521
                     /gene="rpsJ"
                     /locus_tag="VLE22_02530"
     CDS             201..521
                     /gene="rpsJ"
                     /locus_tag="VLE22_02530"
                     /inference="COORDINATES: protein motif:HMM:NF001861.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HSB13304.1"
                     /translation="MLRERIRIRLKAYDHRILDESTKAIVKTAGDTGAQVAGPIPLPT
                     VRNRYTVLRSPHVDKKSREQFEIRTHKRLLDILEPTQDTVDALMKLDLPAGVDVEIKA
                     FGKR"
     gene            596..1228
                     /gene="rplC"
                     /locus_tag="VLE22_02535"
     CDS             596..1228
                     /gene="rplC"
                     /locus_tag="VLE22_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HSB13305.1"
                     /translation="MSPGILGKKIGMTQVFRPDGQVVPVTLLKAGPCVVVQRKTPVTD
                     GYDAVQLGLMEFAKAKRTTKAVAGHLKKSGGEGVKFLREFRLKPGDDDLKTGDRVLVD
                     QFKPADKVDVIGVSKGRGFAGLVKRHHFGGGDASHGSMFHRAAGSIGASSYPSRVWPG
                     QLMAGHMGVQRVTVRNLEVIDVDTEDNVLIVKGAVPGPNGGYVLVRRAKR"
     gene            1234..1860
                     /gene="rplD"
                     /locus_tag="VLE22_02540"
     CDS             1234..1860
                     /gene="rplD"
                     /locus_tag="VLE22_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HSB13306.1"
                     /translation="MPKVDVVDLNNKKVGEVELSDEVFAAPVKDALIYEAVRQYMAGQ
                     RSGTAKTKVRREVSGSGKKLWKQKGTGRARMGSIRSPIWRHGGTVHGPVPRDYSYRLP
                     RKMMLGALRSALTAKLRDGEMTVVQAFSLPDHKTKSVRSVLGNLEVRKTVLLVDNEDN
                     RNLALASRNLPGVTLLPTKDVTVYHLLEHERVLLSEPAARKLSEALSK"
     gene            1857..2144
                     /locus_tag="VLE22_02545"
     CDS             1857..2144
                     /locus_tag="VLE22_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HSB13307.1"
                     /translation="MNIYEVIRRPIVTEKGVEKKEGERTLCFEVTPEANKIQIKAAVE
                     KLFKVKVEEVRTANNAGKLRRRGRFTGYRPDTKKAYVRLKAGEKMPEYAEI"
     gene            2146..2976
                     /gene="rplB"
                     /locus_tag="VLE22_02550"
     CDS             2146..2976
                     /gene="rplB"
                     /locus_tag="VLE22_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HSB13308.1"
                     /translation="MPMKSFRPTTASRRFMSVVSRQEITKETPEKSLVAGKKRTGGRA
                     SHGRVSSRFRGGGHKKAYRVVDFKRDKAGVPAKVAAIEYDPNRSARIALLNYSDGEKR
                     YILCPLGLQVGRTVMSGPDADILVGNALPLKSIPAGTVVHNVELKPGKGGQMARAAGS
                     QVQLVSREGGYALLKLPSGEVRKVPVDCMATIGQVGNTDHENVSLGKAGRKRWLGRRP
                     HNRGVSMNPVDHPHGGGEGKTSGGRHPVTPWGQPTRGFKTRNNKRTEKWIVTRKSKKR
                     "
     gene            2980..3270
                     /gene="rpsS"
                     /locus_tag="VLE22_02555"
     CDS             2980..3270
                     /gene="rpsS"
                     /locus_tag="VLE22_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HSB13309.1"
                     /translation="MGRSVHKGPFCDDHLVRKMEALNAKNEKKVVRTWSRRTTILPEF
                     IGHTIAVHNGKKFIPVYITENMVGHKLGEFSPTRTFKGHAAKAASEKSSKPA"
     gene            3344..3679
                     /gene="rplV"
                     /locus_tag="VLE22_02560"
     CDS             3344..3679
                     /gene="rplV"
                     /locus_tag="VLE22_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HSB13310.1"
                     /translation="MEARAEARYIRVSAQKARLVVDLIRGRQAGEALNLLRATNKRVA
                     PLVEKVVRSAIANAQNRSTDVDVDKLVIREAYVNEGPRAKRIRPAPMGRAYRYQRRMA
                     HIAVTVAEK"
     gene            3699..4649
                     /gene="rpsC"
                     /locus_tag="VLE22_02565"
     CDS             3699..4649
                     /gene="rpsC"
                     /locus_tag="VLE22_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HSB13311.1"
                     /translation="MGQKVHPYGFRLGFTKTWRSRWFATHDYKKLLQEDLDLKESLRS
                     RLKAAGVSSVEVDRPGNKLRVTIRTSRPGIIIGRKGAEIEKLKLELAKRTKRDVFIDI
                     QEVHKPELDAQLVAESIALQLEKRVAFRRAMRKAVDSALRFGCKGIKVRVSGRLNGAE
                     IARSEWYLQGQLPLHTLRADIDYGFAQAYTTYGVIGIKTWVYRGEILEGQMRPGSLLG
                     EPEPRRTPPRDRRERRTPPPVQAAPPPEPAGPAVQAAPGELPQPARASTGPVIPPLAP
                     PQPSWKQEVKETQPDRPEAPGPETEPGTGSNEPPSGGAAK"
     gene            4672..5085
                     /gene="rplP"
                     /locus_tag="VLE22_02570"
     CDS             4672..5085
                     /gene="rplP"
                     /locus_tag="VLE22_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HSB13312.1"
                     /translation="MLMPKKVKYRKQQRGRMKGKAWTGSQLAFGDFGLKATRCAWVTD
                     RQIEAARVAMTRFIKRGGKVWIRVFPDKPITKKPAETRMGKGKGAPEQWVTVIRPGRI
                     LFEMEGVPFAVAEEAMKLAAHKLPIPCKLVARQAG"
     gene            5098..5316
                     /gene="rpmC"
                     /locus_tag="VLE22_02575"
     CDS             5098..5316
                     /gene="rpmC"
                     /locus_tag="VLE22_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HSB13313.1"
                     /translation="MKAEKVRDLDTNELQSQLREMTEQLFRLRFQFSMGQTEGLKKYR
                     VLRKDRARMLTVLRERESASAGAPAEKR"
     gene            5324..5674
                     /gene="rpsQ"
                     /locus_tag="VLE22_02580"
     CDS             5324..5674
                     /gene="rpsQ"
                     /locus_tag="VLE22_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HSB13314.1"
                     /translation="MAKAKGETRRNEKVGQVVSTKAAKTIVVEVTRRVQHPLYKRIVT
                     RRKKFHAHDEAGMARLGDSVRIIESRPLSRLKRWRLAEVLRRAVQVGVEPENLDTFAG
                     SGVAEKSPGQEKKS"
     gene            5671..6039
                     /gene="rplN"
                     /locus_tag="VLE22_02585"
     CDS             5671..6039
                     /gene="rplN"
                     /locus_tag="VLE22_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HSB13315.1"
                     /translation="MIGMRTLLEVADNSGARKVQCILPRGGDKGLRAGLGDVITASVK
                     EAAPDSQIKKGKVVRCVIVRMRKETRRKDGTYIRFDSNAAVLVTDVNEPVGTRVFGPV
                     ARELRDKRFMKIVSLAPEVI"
     gene            6039..6416
                     /gene="rplX"
                     /locus_tag="VLE22_02590"
     CDS             6039..6416
                     /gene="rplX"
                     /locus_tag="VLE22_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HSB13316.1"
                     /translation="MATMTSRKRRQEAPKLRVEIRKNDIVRVIAGRDKGKEGRVLSVD
                     RRKGRLLVEHVMMVKRHTRPNPSRQIKGGIAEREGPIAVSSVMIVCPACGPVRIAHKV
                     DATGGKARRTRVCRKCGAPLDKK"
     gene            6493..7035
                     /gene="rplE"
                     /locus_tag="VLE22_02595"
     CDS             6493..7035
                     /gene="rplE"
                     /locus_tag="VLE22_02595"
                     /inference="COORDINATES: protein motif:HMM:NF000585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HSB13317.1"
                     /translation="MAARLKEHYKKNVVQALVKEFGYANVMAVPKLAKISVNIGLGEA
                     TQNPKLLDGAVSELALIAGQRPIITKAKKSIAAFKLREGMSIGCTVTLRGERMYEFLD
                     RLVNVALPRVRDFRGVSTKSFDGRGNYTLGLKDQLIFPEIDYNKVEKIKGMNISITTT
                     AGSDAEGLALLKHLGMPFRQ"
     gene            7055..7264
                     /locus_tag="VLE22_02600"
     CDS             7055..7264
                     /locus_tag="VLE22_02600"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HSB13318.1"
                     /translation="MATTAKIAKDRRLLKAKTTKKPKLRCRHRNRCFRCGRPRGYLRK
                     FGICRICFRQLALAGEIPGVIKASW"
     gene            7285..7680
                     /gene="rpsH"
                     /locus_tag="VLE22_02605"
     CDS             7285..7680
                     /gene="rpsH"
                     /locus_tag="VLE22_02605"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HSB13319.1"
                     /translation="MTSDPIADMLTRVRNALGARHTKVDVPASRLKVDIAKILKDEGY
                     IANYKMAEEGARRLVRIYLKYTPENQPVISNIERVSRPGCRVYVGRKEIPRVLGGLGI
                     NILTTPRGVMTGRKAHKEGVGGEILCQVW"
     gene            7724..8260
                     /gene="rplF"
                     /locus_tag="VLE22_02610"
     CDS             7724..8260
                     /gene="rplF"
                     /locus_tag="VLE22_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSB13320.1"
                     /translation="MSRVGKKPISLPKGVKVQIDEMLLFEGPKGKLTVPLPDGIHARQ
                     EDATLHIARDADRYAAFHGLTRALASNAVQGVSKGFAKELDIVGIGYRADVKGRIATF
                     SLGYSHPIEVLLPEGIDLKIDKQTHIVISGADRQLVGQVAANIRALRPPEPYKNKGVR
                     YTGEALRKKVGKTGAGAK"
     gene            8257..8619
                     /gene="rplR"
                     /locus_tag="VLE22_02615"
     CDS             8257..8619
                     /gene="rplR"
                     /locus_tag="VLE22_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HSB13321.1"
                     /translation="MIQRTSKNQNRLGIHKRIRRKVHGTQERPRLCIFRSIHHIYVQV
                     IDDASGHTLVSASSADKGAGVAGGGNVAGAKTVGRLVAERAMEKGIRKVVFDRGGYLY
                     HGRVKALAEAAREAGLEF"
     gene            8624..9136
                     /gene="rpsE"
                     /locus_tag="VLE22_02620"
     CDS             8624..9136
                     /gene="rpsE"
                     /locus_tag="VLE22_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HSB13322.1"
                     /translation="MATTPTKRISPQNLNLKEQVVSINRVTKVVKGGKNLSFSALVVV
                     GDPDAKIVGFGTGKAKEVPAAIKKGIEAAKKNLRKVHVLPTTISHVVLGKFGSGMVLL
                     KPAPEGTGVIAGGPVRAVIQAVGIPNVLTKSIGSHNPHNVVKATIDALEKLRDRSAVA
                     DLRGIPVESL"
     gene            9191..9370
                     /gene="rpmD"
                     /locus_tag="VLE22_02625"
     CDS             9191..9370
                     /gene="rpmD"
                     /locus_tag="VLE22_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HSB13323.1"
                     /translation="MAEKIKIKLVRSPICAPEKHKKIVRALGLRKMNQVVERPDNPGF
                     RGMVAKVPHLLAVVE"
     gene            9384..9845
                     /gene="rplO"
                     /locus_tag="VLE22_02630"
     CDS             9384..9845
                     /gene="rplO"
                     /locus_tag="VLE22_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HSB13324.1"
                     /translation="MNLSQVKPPAGQKHAKKRVGRGMGSGTGKTSARGQKGQHSISGF
                     RLMRGFEGGQMPLHRRLPKRGFTNIFRKEFAIVNVGQLEKLEGDAFDPNRLVEMGAVK
                     NLRDGLKILGAGELTRKISVRAHLFSKSALEKIQAAGGSAEVIGRRQPAGE"
     gene            9850..11250
                     /gene="secY"
                     /locus_tag="VLE22_02635"
     CDS             9850..11250
                     /gene="secY"
                     /locus_tag="VLE22_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HSB13325.1"
                     /translation="MNKFFEAIANVFRIPDLRRRVLFTLGLLAVYRIGGHIPTPGINA
                     LRLEEFFQQNQGTLFGFIDLFSGGMFRRLTIFALGIMPYITASIILQLLTVVIPTLEK
                     LQKEGELGRRKITQWTRYLTIGLSMMQSFGIATALQTSQGNFVLNPGWGFILMTMLTL
                     TTGTAFIMWLGEQITDRGIGNGMSLIIFAGIVVGLPKAIGEIYTNVFVTRQWNAIQTT
                     IILAVMVAVVAFIVLVERGERRIPVQYAKRVVGRRMMGGQATHLPLKVNAGGVIPVIF
                     ASSILAFPQTLALVPFVKNNPWLSGMLSAMGNREPLYVVLFVAGIIFFCYFYVSIIFN
                     PNEAADNMRKYGGFIPGIRPGKPTADYINKILTKITMVGGLYLSLLCLIPQIMISGIA
                     LQHLPLAGNWIDAHFPRWLLDGLGVTFYFGGTSLLIVVGVAMDTVNQIEAQLIMRHYE
                     GFTPRAGRIRGRRSVF"
     gene            11289..11963
                     /locus_tag="VLE22_02640"
     CDS             11289..11963
                     /locus_tag="VLE22_02640"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSB13326.1"
                     /translation="MSAGNNSQIALVLFGPPGSGKGTQAKILQASLGIPHVSTGDMLR
                     EHVQADDEIGREVKVIMQAGRLVPDDLVNRLIEKRINRPDTEGGVLLDGYPRTLEQAD
                     KLTGLLRARGVAEVVVHLRVDYNKIIARLSGRRQCPKCGTLYNLASNPPKSEGVCDQD
                     GATLIVREDDREPVIRQRLEAYERQTRPVLEYFARTCPRFYEVEGGDAAPETVAAEIR
                     RLVGKS"
     gene            11960..12730
                     /gene="map"
                     /locus_tag="VLE22_02645"
     CDS             11960..12730
                     /gene="map"
                     /locus_tag="VLE22_02645"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSB13327.1"
                     /translation="MIVRKTPAELEKMRRSGLLVCQVLERLSSMVRDGVSTLDLEIEA
                     EKMIQGAGARPAFKGYYVPAIGKKYPFVLCTSVNDEVVHGMPSAKRILAKGDVVSIDT
                     GAELDGYFGDSAVTVPVGEVDERTKRLIEVTRNALELAIDKVRPGNRLFDVCGTVERH
                     VVANGFTIVREFVGHGIGTQLHEEPQVPNYVDRKNENPRLKEGMVLAVEPMVNAGKAD
                     TRILEDGWTAATRDGKYSAHFEHCVAVTSNGPWVLTRS"
     gene            12743..12988
                     /locus_tag="VLE22_02650"
     CDS             12743..12988
                     /locus_tag="VLE22_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13328.1"
                     /translation="MTESEQDPVRRDSRAVEAVVIELLPNAAFRLQLANLAQVIAHAP
                     GALAANFVRVRPQDKVLVELSPHDRTRGRIVKLLKKD"
     gene            13160..13546
                     /gene="rpsM"
                     /locus_tag="VLE22_02655"
     CDS             13160..13546
                     /gene="rpsM"
                     /locus_tag="VLE22_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HSB13329.1"
                     /translation="MARIAGVDLPNKKRALIGLTYIYGIGRTRSMSILHRAGVEPDKK
                     VADLSEDEVNRIRQIIEEEGAVEGDLRKETSLNVKRLMEMGAYRGLRHRRGMPVRGQR
                     THTNARTRKGPRRGTVANKKKSSAKT"
     gene            13553..13960
                     /gene="rpsK"
                     /locus_tag="VLE22_02660"
     CDS             13553..13960
                     /gene="rpsK"
                     /locus_tag="VLE22_02660"
                     /inference="COORDINATES: protein motif:HMM:NF003698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HSB13330.1"
                     /translation="MAKAPAKAGKKKVFKKKEKKVVPSGVVHIQATFNNTIVSVADPT
                     GNVVAWSSAGSLGFRGSRKGTPFAAQQASLTAANKAREVGMRAVEVHVAGPGAGRESA
                     IRALATAGLEVNAIKDVTPIPHNGCRPPKKRRV"
     gene            14100..14732
                     /gene="rpsD"
                     /locus_tag="VLE22_02665"
     CDS             14100..14732
                     /gene="rpsD"
                     /locus_tag="VLE22_02665"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HSB13331.1"
                     /translation="MARYIGPVCRLCRREGMKLFLKGERCHSEKCAIEKRNVPPGQHG
                     KDRRPKIVGYGLQLREKQKARRVYGVLERQFRNQFEKASQMKGITGEQLLSMLERRLD
                     NVIYRMGFGTSRAQARQVVRHGHIQVNGRKVNIPSFVAKVGDLVEVREKSRKNPTILA
                     ARDATAHAPCPNWLDVDREALRGRIIAMPRRDELVQIQLNEQLIVELYSK"
     gene            14806..15870
                     /locus_tag="VLE22_02670"
     CDS             14806..15870
                     /locus_tag="VLE22_02670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HSB13332.1"
                     /translation="MFRGFQKPKRLVANTENLTDRYGMFTAQPFERGFGTTIGNALRR
                     VLLSSIEGAAITAVRIEGVAHEFSPIPGVVEDATDIILNLKQIPFKMTGDGVRTARLV
                     VDHEGEVLSSQIETDADVEVLDRRMHIATVGENGRLNIEMRLKKGRGYVSADRNYDED
                     LPLGYIPIDSVHSPVRKVNFTVENARLGQMTDYDKLTLEAWTNGAISPQDSIGQAAKL
                     LKDHMSIFINFEETPEVVEEQGLVPNQLNEILNRSVEELELSVRSYNCLKNANIQTIS
                     DLVQKTEAEMLRTKNFGRKSLNEIKEILASLNLSFGMKFDSQGRLIMAAGAPAASGPD
                     DEDEPVDETDTDEELGQVSE"
     gene            15881..16336
                     /gene="rplQ"
                     /locus_tag="VLE22_02675"
     CDS             15881..16336
                     /gene="rplQ"
                     /locus_tag="VLE22_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HSB13333.1"
                     /translation="MRHKRGGYKLGRDTAGRRSLLRNLVTSVIEQERVVTTVIKAKAA
                     KPLVDKMITLAKRDSLHARRQAAAYLQTPASVQKLFEKLGTRFGQRNGGYTRIVRLGW
                     RKGDGAEQAMLELVGSELVKRAAERAKRREERLKAIQEGREPDEPATGA"
     gene            16506..16949
                     /locus_tag="VLE22_02680"
     CDS             16506..16949
                     /locus_tag="VLE22_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSB13334.1"
                     /translation="MAITKYDPFRDFEDFPAGLRLFQDTLNRFLSEPATQRPWTPSVD
                     VLETENELILKADLPDVSLKEIEIQIENGTLSLKGERKFEREQNGVGYHRIERSYGTF
                     ARYFSVPDTVDTDKVKADYKNGVLTVTLPKKEVAKPKSIKVEVHE"
     gene            17059..17910
                     /locus_tag="VLE22_02685"
     CDS             17059..17910
                     /locus_tag="VLE22_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HSB13335.1"
                     /translation="MNGVKTVVLLGLLSGLLLVGGEAVAGRNGLYMGLALAVVMNAAS
                     YFFSDKIALAMYRAQPVTQTENGQVYARVAPMVQNLCQRMGLPMPKLWLIPEQSPNAF
                     ATGRNPAHASVAFTAGLLELMDVSELEGVIAHELGHVKNRDILISSVAATIAAAITMV
                     ARMAMFAGMFGGRRDDRDDSGGGLGGLFMLIVAPIAAMLIQMAISRTREYSADATAAQ
                     YTGGPYGLISGLRKLEGWSKRIPMDASPATAHMFIIKPFSGQTLMRLFSTHPSTEDRV
                     RRLEALA"
     gene            17941..19086
                     /locus_tag="VLE22_02690"
     CDS             17941..19086
                     /locus_tag="VLE22_02690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HSB13336.1"
                     /translation="MSRKGADRIASGHPWVFSSDVQDRGGASPGDVVTVADPRGRPLG
                     TAHYSDTSQISLRLLTERFEPVDRSFFAGRLKAASEFRQRVVHDTSAYRLVHGEADGL
                     PGLVVDRYGDFLVIQTLDQGMDRSLPDIVACLEDLCAPSGIVARNDAAVRVRESLPRE
                     STVLTGNVPDRVSVRLNGLTFLVDILRGQKTGFFLDQRENYSATARYARGHALDCFTF
                     EGGFALHLAGGCESVEAVDSSEHALCLARENAGLNGIGNVQFRQADVFDLLAGYSAAG
                     RRFDTVVLDPPGFAKSRSAIEGAARGYKDLNLRALRLLGAGGVLVTCSCSHHVSEAML
                     LEIVASASLDADRKLRVLERRTQALDHPILLTIPETHYLKCVILEVA"
     gene            19266..>19778
                     /locus_tag="VLE22_02695"
     CDS             19266..>19778
                     /locus_tag="VLE22_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HSB13337.1"
                     /translation="MFRSEALPVIQRQASSQKALGRPVTFGLLRTVLVLLSLAVLAAP
                     MFAQEHHGGEANLILPDLDQATFLGGIGGRSLLMVGLLVSGLGLLFGLVIYRRLRDMP
                     VHAAMREVSELIYETCKTYLATQGKFLMMLEALIAVIIVFYFGFLQGFEAFRVGIILL
                     FSVVGISGSFL"
BASE COUNT         4166 a         5692 c         6482 g         3438 t
ORIGIN      
        1 ggagatggtg atgcccgggg acaacgtgaa cctgacggtg gagttgatca cgccggtggc
       61 gatggacaag ggcttgcggt ttgcgatccg cgagggcggg cgcaccgtcg gcgccggtac
      121 ggttacggaa gtcttggagt agaggcaagc cgccggcgca ctcgtgagtc cgctggcggc
      181 gcgcccctac agagggcacg atgctcagag aacgcattcg aattcggttg aaggcatacg
      241 atcaccgcat cctcgacgag tcaacaaagg ccatcgtaaa gacggccggc gacacggggg
      301 cgcaggtggc gggaccaatt ccgctgccga cggtgaggaa ccgctacacg gtcctccggt
      361 cgccgcacgt ggacaaaaag tcccgagagc agtttgagat ccggacccac aagcggctgt
      421 tggacatcct ggaaccgacc caggacacgg ttgatgcgct gatgaagctc gacctcccgg
      481 cgggcgtgga cgtggagatc aaagcgtttg gaaaaagata gtgcgtggcc cggcgccgtt
      541 ggcggcggcg gaagcgccgg ctagcggacg gatgccacag gccagttgag gaattatgag
      601 cccaggaatt cttggcaaaa agatcggtat gacgcaggtg ttccggccgg atgggcaggt
      661 tgtaccggtg acgctgctca aggcgggccc ttgcgtggtg gtgcagcgga agacgccggt
      721 cacggacggc tacgatgccg tgcaacttgg cctcatggag ttcgccaagg cgaagcggac
      781 gactaaggcc gttgccggac atctgaagaa gagcggcggc gagggagtca agttcctgcg
      841 cgagttccgc ctgaagccag gtgacgacga tctcaagacc ggcgaccggg tgctggtcga
      901 ccaattcaaa ccggccgaca aagtggacgt aatcggagtg agtaaagggc gcggattcgc
      961 tggtctcgtg aagcgccatc acttcggcgg cggcgacgcc tcgcacggtt ccatgttcca
     1021 ccgcgcggcg ggttcgatcg gcgcatcgag ctacccttct cgggtctggc cgggccagct
     1081 catggccgga cacatgggcg tgcagcgcgt cacggtgcgg aacctggaag tgattgacgt
     1141 ggataccgag gacaacgtgc tcattgtcaa gggcgccgtc cccgggccga acggcggcta
     1201 cgttttagtc cgccgtgcga agaggtgaac gccatgccca aggtggacgt cgtcgacttg
     1261 aacaataaga aggtcggaga ggttgagctc tccgacgagg tgtttgccgc acccgtcaag
     1321 gatgcgttga tttatgaagc cgtgcggcaa tacatggctg gtcaacgaag cggcacggcc
     1381 aagaccaaag tgcgccggga agtttcaggc tccggcaaga agctgtggaa gcagaaagga
     1441 accgggcggg cccgcatggg ttccatccgt tctccaatct ggcgtcacgg cggcacggtg
     1501 cacggaccgg tgccgcggga ttacagctac cgtctgccgc ggaagatgat gctcggcgcc
     1561 ttgcggtccg ctctcacggc gaagctgcgc gacggagaga tgacggtggt gcaggcgttc
     1621 tcgcttcccg accacaagac aaaatccgtc cggtccgtac ttggaaacct cgaggtccgc
     1681 aagacggtac tgctggtgga caacgaggac aaccgaaacc tggcgctggc gtcccggaac
     1741 ctgccgggcg tgacgctgct gccgacgaaa gacgtcaccg tataccacct gctcgagcat
     1801 gagcgcgttc tgctgtctga gccagcggcc cggaaactat cggaggccct gtcgaaatga
     1861 acatctacga agtcatccgc aggcccatag tgaccgaaaa gggcgtcgag aagaaggagg
     1921 gcgagcgcac cctctgtttc gaggtcactc ccgaagccaa caagatccag atcaaggccg
     1981 ccgtcgagaa gctgttcaag gtgaaggtgg aggaagtacg gacggcaaac aacgccggca
     2041 agctgcggcg gcgcggccgg ttcacgggat atcgtccgga caccaagaag gcttacgtcc
     2101 ggctcaaggc cggcgaaaaa atgccggagt acgcggagat ctgacatgcc gatgaagagt
     2161 tttcgtccga cgacggccag ccggcgtttc atgtcggtgg tttcccgcca ggagatcacc
     2221 aaggagacgc cggagaagtc gcttgttgcc ggcaagaagc gcaccggtgg gcgcgccagc
     2281 cacgggcggg tcagctcgcg cttccgtggc ggcgggcaca agaaggcgta ccgggttgtc
     2341 gatttcaagc gggacaaggc gggcgtaccc gccaaagtag cggctatcga atacgatccc
     2401 aaccggtcgg cgcggatcgc cctgctgaac tatagcgacg gcgagaagcg gtacatcctc
     2461 tgcccgctcg ggctacaggt gggccgtacg gtgatgtcgg gtcccgatgc ggacatcctc
     2521 gtcgggaacg cgctaccgct caagagtatc cccgccggca ccgttgttca caacgtcgag
     2581 ttgaagccgg gcaagggcgg gcagatggcg cgtgcggcgg gatcgcaggt gcaactcgtg
     2641 tccagggaag gcggctacgc tcttctgaag ctgccgtccg gcgaagtccg gaaggtgccg
     2701 gtggactgca tggccaccat cgggcaggtt ggcaacaccg accacgaaaa cgtgtcgctc
     2761 ggcaaagcgg gacggaagcg atggctcgga cgcaggccac acaaccgggg cgtgtcgatg
     2821 aaccccgtag atcatccgca cggcgggggc gagggaaaga cttcgggcgg acgtcacccg
     2881 gtgactccgt ggggccagcc gacccgtggt ttcaagacgc gcaacaacaa gcggaccgag
     2941 aagtggatcg taacgcgtaa gtcgaaaaag agatagctta tgggccgttc tgtacacaag
     3001 ggaccgttct gtgatgacca tcttgttcgc aagatggagg cgctgaacgc gaagaacgag
     3061 aagaaggtcg tgcggacctg gtcgcggcgc acaacaatcc tgcccgagtt catcgggcac
     3121 acgatcgccg tgcacaacgg gaagaagttt atcccggtgt atatcacgga gaacatggtc
     3181 gggcacaaac tgggcgaatt ttcgcccacg cgtaccttca agggccacgc ggccaaggcg
     3241 gcgagcgaga agtcgtcgaa gcccgcgtaa gaaggaggca tgtggcgcgc agtcagccgc
     3301 ctggacggcg gatgcagcag gcggtgagcc acaagtgaga gacatggaag cgagagcgga
     3361 agcaagatac attcgggttt cagcgcagaa agcgcgcctg gtggtcgatc tgatccgggg
     3421 ccggcaggcc ggcgaggccc tgaatttgct gcgggcgacc aacaagcggg tcgcgccatt
     3481 ggtggagaaa gtggtgcgct cggccatcgc caacgcgcag aaccggtcca ccgacgtcga
     3541 tgtcgacaag ctcgtaatcc gcgaagcgta cgtcaatgaa gggccccgcg ccaagcggat
     3601 ccgcccggcg cccatgggcc gagcctaccg ttaccagagg cgaatggccc acattgccgt
     3661 gacggtggcg gagaaatgaa gaccacggaa cggtagatat gggacagaaa gttcatccat
     3721 acggatttcg gctcggtttc accaagacct ggcggtcccg ttggttcgcc acgcacgact
     3781 acaagaaact gctccaggaa gacctcgatc tgaaggaatc gctccgcagc cggctgaagg
     3841 cggcgggcgt cagttcggtc gaagtcgaca ggcccggcaa caagttgcgg gtcacgatcc
     3901 gcacgtcgcg gccggggatc atcatcggac gcaaaggcgc cgagatcgag aagctcaagc
     3961 tggaactggc gaagcggacc aagcgcgatg tctttatcga catccaggag gtccataagc
     4021 ccgagctgga cgctcagctt gtggccgaat cgatcgccct ccagctcgag aagcgcgttg
     4081 cgttccgccg tgcgatgcgg aaagccgtcg attccgcctt gcgcttcggc tgcaagggca
     4141 tcaaagtccg cgtctcggga cggttgaacg gcgcggaaat cgcccggagc gaatggtact
     4201 tgcagggcca gttgcccctg cacacgctgc gggcggacat cgactacggt ttcgcgcagg
     4261 cttatacgac gtatggcgtg atcggtatca aaacctgggt ttacagggga gagatcctcg
     4321 agggccaaat gcggccagga tcgctgctgg gagagccgga accgcggcgt accccgccgc
     4381 gtgaccgacg cgagcgcagg acgccgccgc cggtacaagc tgctcctcct cccgagccgg
     4441 ccggtccggc tgtacaggca gcgccaggtg aattgcctca gccggcgagg gcctcgacgg
     4501 gccccgtgat cccaccgctg gcgccgcctc aaccgtcgtg gaagcaggaa gtgaaagaaa
     4561 cccaaccgga tcgcccggaa gcacccggcc ccgagacgga gcccggaacc ggatcgaacg
     4621 agcccccgtc cggcggggcc gccaagtagg atggcccgga ggtaaaacgc catgttgatg
     4681 ccgaagaaag tcaagtacag aaagcagcag cgaggccgca tgaagggcaa ggcctggacc
     4741 ggctcccagc tcgcttttgg cgacttcggg ctcaaggcga cacggtgcgc ctgggtgacg
     4801 gaccgccaga tcgaggctgc ccgcgtcgcg atgactcgtt tcatcaagcg cggcggcaag
     4861 gtttggatac gcgtttttcc ggacaagccg atcacgaaga aaccggccga gacccgcatg
     4921 ggaaagggca agggagctcc ggagcagtgg gtcaccgtaa tccggcccgg gcggattctg
     4981 ttcgagatgg aaggcgtgcc attcgcggtg gccgaggaag ccatgaagct ggcggcgcac
     5041 aagctgccga ttccgtgcaa gctggtggcg cgtcaggcgg gctgagagga gagaacgatg
     5101 aaagcggaaa aagtccggga tctggacaca aacgaactgc aaagccagct tcgggaaatg
     5161 acggagcagt tgttccgtct ccgtttccag ttctcgatgg gccagacaga agggctgaaa
     5221 aaataccgcg ttcttcggaa ggaccgagcc agaatgctca cggtcctgcg cgagcgcgag
     5281 agtgcgtcgg cgggtgcgcc ggccgagaag aggtaaccga gctatggcca aggcgaaggg
     5341 ggagacccgc aggaacgaga aggtcggcca ggtcgtttcg accaaggcgg cgaagacgat
     5401 cgtcgtggaa gtgacccggc gcgtgcagca ccctctgtat aagcgaattg ttacgcgccg
     5461 aaagaagttt cacgcgcacg acgaggcggg tatggcccgt ctcggagaca gcgtgcggat
     5521 tatcgagtcc cggccgctca gccggttgaa gcgctggcgg ctagctgagg tcctccgccg
     5581 tgccgtccag gtgggagtgg agccagagaa cctggatacg ttcgccggct cgggcgtggc
     5641 ggagaaatct ccgggtcagg agaagaagtc atgatcggaa tgcgaaccct gctcgaagtg
     5701 gcagacaaca gcggcgctcg aaaggttcag tgcatcctgc cccgcggcgg cgacaagggc
     5761 ctgcgggcag gcctcggcga cgtgatcacg gccagcgtga aggaagccgc tccggattcc
     5821 cagatcaaga agggcaaggt cgttcgctgc gtgatcgttc gcatgcgcaa ggaaacgcga
     5881 cgcaaggatg ggacctacat ccgtttcgac agcaacgcgg ccgttctggt aacggatgtc
     5941 aatgaaccgg tggggacccg cgtgttcggc cctgtcgcca gagaactgcg agacaagcgg
     6001 tttatgaaga tcgtgtcgct ggcgccggag gtgatttgat ggcgacgatg acgagccgga
     6061 aacggcgtca ggaagccccg aaattgcggg tcgagatcag gaagaacgac atcgtgcgtg
     6121 tgatcgcggg ccgcgacaag ggaaaagagg gccgcgtgct cagcgtggac cgccgcaagg
     6181 gcaggcttct tgtcgagcac gtgatgatgg tcaagcgcca cacccgtccg aacccgtcgc
     6241 ggcagatcaa gggcggcatc gccgaacgtg agggcccgat tgcggtgtcc agcgtgatga
     6301 tcgtatgccc ggcctgcggg ccggtgcgga ttgctcacaa agttgatgcg accggcggca
     6361 aagcacggcg tacgcgcgtg tgccggaagt gcggcgcgcc gctcgacaag aagtagggga
     6421 atgaaacaag gaaacatcgc tgagttgact ttgcgaatcg atgaagcgat ggaccagtag
     6481 agaaacaggc ctatggctgc cagattgaaa gagcactaca agaagaacgt cgtgcaggcg
     6541 ctagtcaaag agttcggcta cgcgaacgtc atggcggtgc cgaagctcgc caagatttca
     6601 gtgaacatcg gcctcgggga ggcgacgcag aacccgaagc tgcttgacgg cgccgtctcc
     6661 gagctggcgc tgattgccgg acagcggccc attatcacca aagcgaagaa gtcgatcgcg
     6721 gcgttcaagc tccgcgaggg aatgtcgatc ggatgcacgg ttaccctgcg cggcgagcgc
     6781 atgtacgagt tcctggaccg cttggtcaac gtggccctgc cccgagtccg cgactttcgc
     6841 ggggtgtcga ccaagtcttt cgacgggcgc ggcaactata cgctcggcct gaaagaccag
     6901 ttgatttttc cggagattga ttacaacaag gtggaaaaga tcaagggcat gaacatcagc
     6961 atcacgacca ccgccgggtc ggacgccgaa gggcttgcgc tcctgaagca ccttgggatg
     7021 ccgttccgcc agtagcggag aaaggacaca tctgatggca accaccgcaa agatcgcaaa
     7081 ggaccgcagg ctgctgaagg cgaagaccac gaagaagccg aaactccgct gccgtcaccg
     7141 taaccgctgc ttccggtgcg gccggccacg aggatatctg cgcaaattcg gcatttgccg
     7201 gatttgtttc cggcagctcg cgctcgccgg ggagatcccc ggcgtcatca aggcgagctg
     7261 gtaagtgagg aggggaaggt aaggatgaca tcggatccga tcgccgacat gctcacgcga
     7321 gtccggaatg ccttgggcgc gcgccacacg aaggtggatg tgcccgcttc ccggctgaag
     7381 gtcgatatcg ccaagatcct caaggacgag ggctatatcg ccaactacaa gatggccgag
     7441 gagggcgccc ggagactcgt ccggatatac ctgaagtaca cgcccgagaa ccaaccggtg
     7501 atctcaaaca ttgagcgagt gtcccgcccc ggctgccggg tgtacgtggg ccgcaaggag
     7561 attccgcggg tcctcggcgg cttgggcatc aacatactga cgaccccacg cggcgtgatg
     7621 accggccgca aggcgcacaa ggaaggcgtg ggaggggaga ttctttgtca ggtctggtag
     7681 gcggccgcaa ggctcctttg accagacact gaggtaacaa gtcatgtcga gagttggaaa
     7741 aaagccgatc tcgttgccaa aaggcgtcaa ggtccagatc gacgagatgc tgctgttcga
     7801 aggtccgaag ggcaagctca ctgtccccct tccggacggc attcacgcgc ggcaggagga
     7861 cgccactctg cacatcgccc gcgacgctga cagatatgcc gcatttcacg ggttgacacg
     7921 ggcgctggca tcgaacgccg tgcaaggcgt ttccaaaggc ttcgccaagg agctggatat
     7981 cgtcggtatc ggttacaggg ctgacgtcaa gggacgaatc gccacgttct cccttggtta
     8041 ttcgcatccg atcgaggtgc tgctgcccga gggaatcgac ctcaaaatcg acaagcagac
     8101 gcatatcgta atcagcggcg ccgaccggca gcttgtgggc caggtcgcgg ccaacatccg
     8161 cgccctgcgg ccgcccgagc cctataagaa caagggcgtc cggtacaccg gcgaagcgtt
     8221 gcgcaagaag gtcggcaaga ccggtgcggg ggccaaatga tccagagaac gtcgaagaat
     8281 cagaatcggc ttggtattca caagcggatc cgccgcaagg tgcacggcac gcaggaacgg
     8341 ccgaggctgt gcatcttccg cagcattcat cacatttacg tgcaggtcat cgacgatgcg
     8401 tccggccaca cgctggtgtc ggcctcgtcc gcggacaagg gagcgggcgt cgccggcggc
     8461 ggcaatgtcg ccggtgcaaa gacagtcggc cggctggttg ccgagcgggc aatggaaaaa
     8521 ggcattcgca aggtcgtttt cgatcgtggc ggctacctct accacggtcg cgtgaaagct
     8581 ctggccgagg ccgcgagaga agcgggattg gagttctagg ggcatggcaa cgacaccgac
     8641 aaagcggatc agcccgcaga acctgaatct gaaggaacag gtcgtctcga tcaatcgtgt
     8701 caccaaggtg gtcaagggag gaaagaacct gagtttctcc gcccttgttg tggtcggtga
     8761 tccggacgca aagatcgtcg gcttcggcac cggaaaagcc aaggaggtgc cggcggcgat
     8821 taagaagggc atcgaagcgg cgaagaagaa tctgcgcaag gtccatgttc ttccgacaac
     8881 gatttcgcac gttgtgttgg gcaagttcgg cagcggaatg gttctactga aaccggctcc
     8941 cgagggcacg ggcgtgattg cgggaggtcc cgtacgagcc gtgatccagg cagtcggaat
     9001 cccgaacgtg ctgacaaagt cgatcggttc gcataaccct cacaatgtcg tgaaggccac
     9061 gatcgacgcg ctcgagaagt tgcgcgaccg gagcgcggta gccgatctgc gcggcatccc
     9121 ggtggagagt ttgtagtatg agcctcgccg cggcccggcc gcggcggacg attggctgga
     9181 aagcttggtt atggcggaaa agatcaagat caagctggtg cgcagcccga tctgcgcgcc
     9241 ggagaaacac aagaagatcg ttcgtgctct gggccttcgg aagatgaacc aggtcgtcga
     9301 acggccggac aaccccggat tccgaggaat ggtggccaaa gtgccgcacc tgctggcagt
     9361 ggttgagtga ggaaacgatc cctatgaacc taagtcaagt gaaaccccct gccgggcaga
     9421 agcacgccaa gaagcgtgtt ggacggggca tgggctccgg caccggcaag acctcggctc
     9481 gcggccaaaa gggacagcac tccatcagcg gcttccggct gatgcgcggg ttcgagggcg
     9541 gacagatgcc gcttcaccgg cgcctcccga agcgcggctt cacaaacatc ttccggaagg
     9601 aattcgcgat cgtcaacgtc ggccagcttg aaaagttgga gggggacgcc ttcgatccga
     9661 atcgcctcgt ggaaatgggc gcggtcaaga acctgcgtga tgggttgaag attttgggtg
     9721 cgggcgagct gacccgaaag atcagcgtgc gggcgcacct gttctcgaag tcggctctgg
     9781 agaagatcca ggcggccggc ggcagcgcgg aagtgatcgg ccgacgccag ccggcaggcg
     9841 agtgagcgta tgaacaagtt cttcgaggcc atcgcgaacg tcttccgtat cccggacctt
     9901 cgcaggcgcg tcctgttcac cctcggcctg ctagcggtct atcggatcgg cgggcacatc
     9961 cccaccccgg gcatcaacgc cttacgcctt gaggagtttt tccagcagaa ccagggtacg
    10021 ctgttcggct ttatcgacct gttttcgggt ggcatgttcc ggcggctcac gatcttcgcc
    10081 ctgggaatca tgccgtacat caccgcctcg atcatccttc agttgctgac ggtcgtaatc
    10141 ccaacccttg agaaattaca gaaggaaggc gagctcggcc gccggaagat cacccagtgg
    10201 acgcgctacc tgaccatcgg cctctcgatg atgcagtcgt tcggtatcgc caccgccctc
    10261 cagacaagcc aggggaattt tgtgctgaac ccgggctggg gcttcatcct gatgacgatg
    10321 ctgacgctga ccacggggac cgcatttatc atgtggctcg gcgagcaaat caccgaccgc
    10381 gggatcggca acgggatgtc gctgatcatc ttcgcgggta ttgttgttgg cctgcccaaa
    10441 gcgatcggtg agatctatac gaacgtgttc gtcacgcggc agtggaacgc gattcagact
    10501 accattatcc tggcggtgat ggtggcggtt gtggccttca tcgtgctcgt cgagcgcggc
    10561 gaacggcgaa tcccggtgca gtatgccaag cgggtggtcg ggcggcggat gatgggcggc
    10621 caggcgacgc atctgccgtt gaaagtcaat gccgggggcg tgattccggt gatcttcgct
    10681 tcgtcgatcc ttgcgtttcc gcagacgctg gcgttggtgc cgttcgtaaa gaacaatccg
    10741 tggttgagtg gaatgctctc ggcgatggga aaccgggagc cgctctacgt cgtgctattc
    10801 gtcgccggca tcatcttctt ctgctacttc tacgtctcca tcatctttaa cccgaacgag
    10861 gcggccgata acatgcgcaa gtacggcggg ttcatcccgg gcatccgccc cggcaagccc
    10921 accgccgact acataaacaa gatcctgacc aagatcacca tggtcggagg cttgtacctg
    10981 tcgctgctgt gcctgatccc gcagatcatg atctccggga tcgccttgca gcacctgccg
    11041 ctggccggca actggattga cgcgcatttc ccccgctggc tgctggacgg tctcggggtc
    11101 acgttttact ttggcggcac gtccctgctc atcgtggtcg gcgtcgctat ggatacggta
    11161 aaccagattg aggcgcaatt gatcatgcgc cactacgagg gattcacgcc tcgtgccggc
    11221 agaatccgcg ggcgccgaag cgtcttctag atcggcgggt accgcagtgt tcgcaggcgt
    11281 ctctgttcat gtccgcaggt aacaatagcc agatcgctct ggtgctgttc ggtccgcccg
    11341 gctccgggaa gggcacgcag gcgaagatct tgcaggcgtc cctcggaatt ccgcatgtct
    11401 cgacgggcga catgctgcgg gaacacgtgc aggccgacga cgaaattggc cgggaggtca
    11461 aggtcatcat gcaggccggc aggctcgttc cggacgacct ggtgaatcga ctgatcgaga
    11521 agcggatcaa ccggccggac accgaaggcg gagtgttgtt ggacggatat ccccggacgc
    11581 tggagcaggc agacaaactg accggcttgc tgcgggcgcg aggcgtcgct gaagtggtgg
    11641 tccacctcag ggtggattac aataaaatta tcgctcgtct ctcaggccgc cggcagtgtc
    11701 cgaagtgcgg aacgttgtac aacctggcgt cgaacccgcc gaaaagtgaa ggggtgtgcg
    11761 atcaggatgg cgcaacgttg attgtgcgcg aagacgaccg ggagccggtg atacgccagc
    11821 ggctggaggc gtacgagagg cagacccgtc cggtgctcga gtatttcgca cggacctgtc
    11881 cgaggtttta cgaagtggag ggtggcgacg cagcgccgga aacggtcgcc gcggagatcc
    11941 ggaggctggt tgggaagtca tgatcgtccg caagactccg gccgagctcg agaagatgcg
    12001 ccgcagcgga ctgctggtat gtcaggtgct cgaacggttg tcctcaatgg tccgcgacgg
    12061 agtgagcacg ctcgatcttg agatcgaagc ggagaagatg atccaaggcg cgggcgcccg
    12121 gcctgctttc aagggctact atgtgccggc catcggaaag aagtatccgt ttgtgctttg
    12181 cacctcggtg aacgacgagg tggtgcacgg gatgccgtcg gcgaagagga ttttagccaa
    12241 gggcgacgtg gtttcgatcg acacgggcgc ggaactcgat ggctacttcg gcgactcggc
    12301 ggtaaccgtt ccggtcggtg aagtggacga acggacgaaa cggctgatcg aagtgacccg
    12361 gaatgcgttg gaattggcga ttgacaaggt ccggccgggg aatcggctgt ttgatgtttg
    12421 cgggacggtg gagcgccacg ttgtggcgaa cgggtttacg atcgtccgcg agtttgtggg
    12481 gcacggcatt ggtacgcagc ttcacgaaga gccacaggtc ccgaactatg tggaccgcaa
    12541 gaacgagaat ccgcggttga aggaaggcat ggtgctggcg gtcgagccga tggtgaacgc
    12601 gggaaaggcc gatacccgga ttctggaaga cggctggacc gcggcgacgc gggacggaaa
    12661 atactcggcg cacttcgagc actgcgtggc cgttacgtcg aatgggccgt gggtgcttac
    12721 gcggtcctga ggcctccctg gaatgacgga gtcggagcag gacccggtcc ggcgcgacag
    12781 ccgagcggtt gaggcggtgg tgatagagtt gctgccgaac gcggccttcc ggctgcagtt
    12841 ggcgaacctg gctcaggtga tcgcgcacgc gccgggagcg ctcgccgcga attttgttcg
    12901 cgtgcggccg caggacaagg ttttggttga gctgtcgccg catgacagga cgcgggggcg
    12961 gatcgtgaag ctgttgaaaa aggattagcc ggcggcggaa tcttcgcgat tccgggacgc
    13021 cggaggcaag cttatgaaag tacgggcttc cgtaaaaaag atttgtgata aatgcaaggt
    13081 gatccaccgc aatggtgtgg tgcgggtcat ctgcacgaac ccgaagcaca aacagcgcca
    13141 gggatagcag gaggatcaga tggcacgtat tgcaggcgtg gacctgccga acaagaagcg
    13201 ggccctcatc ggcctgacct atatttatgg gatcgggcgg acacggtcga tgtcgattct
    13261 gcaccgtgcc ggcgtcgagc ccgataagaa ggtcgccgac ctcagtgaag acgaggtgaa
    13321 tcgcatccgc cagatcatcg aggaagaggg cgcggtcgag ggtgacctgc gcaaggaaac
    13381 ctcgttgaac gtcaagcggc tcatggagat gggggcttac cgtgggctgc gccaccggcg
    13441 tggaatgccc gttcgcggcc agcgcaccca caccaacgcc cgcacgcgca agggacctcg
    13501 gcgcggcacg gtcgcgaaca agaagaaatc cagcgcgaag acttaggaac atatggccaa
    13561 agcaccagcg aaagcaggaa agaagaaggt cttcaagaag aaggaaaaga aggtcgttcc
    13621 gtccggtgtc gttcacatcc aggcgacgtt caacaacaca atcgtcagcg tcgccgatcc
    13681 gacgggaaac gtggttgcct ggtccagcgc gggttctctt ggttttcgcg gttcgcgcaa
    13741 gggaacacca tttgccgcgc agcaggcgtc gcttacggcc gccaataagg cgcgggaagt
    13801 cgggatgcgc gcggttgaag tccatgtagc cggccccggc gcgggccgcg agtcggcaat
    13861 tcgcgccctg gctacagccg gactcgaagt gaatgcgatc aaggacgtca cgccgattcc
    13921 ccacaacgga tgtcgtccgc cgaagaagcg aagagtttga gagagtctgc gggcatgcgg
    13981 catcaggctg cgaagccgga tcatgagcag cggtccgccg cccaagggcg atggaaagca
    14041 ggaggaagct ccctgccagg agcgtaaact gcacctgaga taaggttaag gaggaagact
    14101 tggcacgata tattggcccc gtttgccggc tctgccggcg cgagggtatg aagctgttcc
    14161 tcaagggaga gcgctgccac agcgagaagt gcgccatcga gaagcgcaac gtaccgcccg
    14221 gacagcacgg caaggaccgc cgcccgaaga tcgtgggcta cggcctgcag ctccgcgaga
    14281 agcagaaagc tcgccgcgtt tacggcgtac tcgagaggca gttccgcaac cagttcgaaa
    14341 aagcctccca aatgaagggc atcaccggcg agcagttgct gtcgatgctc gaacggaggc
    14401 tggacaatgt catctatcgg atgggtttcg gcacgagccg ggcccaggcc cgtcaggtcg
    14461 tacgtcacgg acacatccag gtcaacggac ggaaggtcaa catcccgtca ttcgtcgcga
    14521 aagtcggcga cctcgtcgag gtccgtgaga agagccggaa gaacccgacc atcctcgcgg
    14581 ctcgcgacgc taccgcccac gcaccgtgtc cgaactggct cgacgtcgac cgggaggcgt
    14641 tgcgcggccg tattatcgcc atgccgcgcc gagatgaact ggttcagatc cagttgaacg
    14701 agcagttgat cgtcgaattg tactcgaagt agccgggagc ggtttcgccg cgccaatcgg
    14761 cgtggcggag ccgtgggcgc aggattcgac aaaaggacaa gatccatgtt tagaggcttt
    14821 cagaaaccca agcggctggt tgccaacacg gagaacctca cggaccgtta tggcatgttc
    14881 acggcgcaac ccttcgagag agggttcggc accaccatcg gtaatgcact tcgccgggta
    14941 ctgctcagct ccatagaggg cgcggcgatc acggccgtcc gtatcgaggg cgtcgcccac
    15001 gagttcagcc cgatcccggg cgtggtggag gacgccacgg atatcatcct caacctcaag
    15061 cagattccgt tcaaaatgac cggtgacggt gtgcgtaccg cacgcctggt cgtcgaccat
    15121 gagggcgaag tgctgagcag ccagatcgaa accgacgccg acgtcgaggt gctcgatcgc
    15181 cggatgcaca tcgccaccgt tggtgagaat ggccggctca atatcgagat gcggctgaag
    15241 aagggacgcg ggtacgtcag cgcggatcgc aattatgacg aagacctgcc ccttggctat
    15301 attcccatcg attccgtgca ttccccggtt cgcaaggtca acttcaccgt ggagaacgcg
    15361 cggcttggcc agatgaccga ctacgacaag ctcacgttgg aggcctggac caacggagcc
    15421 atctccccgc aggactcgat cggccaggcc gccaagctcc tcaaagacca catgtccatc
    15481 ttcatcaact ttgaggagac acccgaggtc gtcgaggagc aaggactggt tcccaaccag
    15541 ttgaacgaga tcctgaaccg ctccgtcgaa gagctcgaac tgtccgtacg ctcttacaac
    15601 tgcttgaaga acgcaaacat ccagaccatc agcgatctcg tccagaaaac cgaagccgag
    15661 atgctgcgca ccaagaactt cggtcggaag tccctgaacg agatcaagga aatcctcgct
    15721 tcgctgaacc tctcgttcgg aatgaagttc gattcgcagg gccgcctcat catggcggcg
    15781 ggcgcacccg cagcaagcgg cccggatgac gaggacgaac ccgtcgacga aaccgataca
    15841 gatgaagaac tcggccaggt aagcgaatag gaggccagcc atgagacata aacgaggtgg
    15901 atacaaactt ggcagagaca cagcgggcag gcggtccctg ctgcggaacc tagtcaccag
    15961 cgtgatcgag caggaacgcg tagtcacgac ggtgatcaag gccaaggccg ccaagccgct
    16021 agtcgacaag atgatcacgc tggccaaacg ggactccctc catgccaggc ggcaggcagc
    16081 cgcctatctc cagaccccgg catctgtcca gaagctcttt gagaagcttg gcactcgctt
    16141 cgggcagcgg aacggcggct acacccgaat cgtacggctc ggctggcgca agggcgacgg
    16201 cgctgagcag gcgatgctgg agctcgtcgg ctccgagctc gtcaagcgcg ccgcagagcg
    16261 ggccaagagg cgcgaagaac gcctgaaggc gattcaggaa ggacgggagc cagacgagcc
    16321 ggctactgga gcctaaaggt cggtccatct taagttattg aggcgcagcg ggttaactac
    16381 ctgctgcgct tttcgtattt cttggggcgt aaaattttat aagatgactt gaatatcact
    16441 tgacgaatag acactcaggt tatattatta gatcaggatt tggtttgaag gagggaaacc
    16501 aagctatggc gattaccaaa tatgatccat tccgtgattt cgaagacttt ccggccgggc
    16561 tccggctctt ccaggacacg ctgaaccggt ttctgagtga gccggcgacc cagcggccgt
    16621 ggacgccgtc cgttgacgtc ctcgagactg aaaacgaact gatcctcaag gcagatctcc
    16681 ccgatgttag cttgaaggag atcgagatcc agattgaaaa cggcaccctc agcctcaagg
    16741 gcgaacgcaa gttcgagcgc gaacagaacg gcgtcggcta ccatcgcatc gagcgcagct
    16801 acgggacatt cgcccgctac ttctcggtcc ccgataccgt cgataccgac aaggtgaagg
    16861 cggactacaa gaacggcgtg ctcaccgtca cactgccgaa aaaggaagta gccaagccga
    16921 agtccatcaa ggtcgaggtt cacgagtaac cgcttgaggc ggtttaccag ggggggcgtc
    16981 gcatggcggc gcctctttcg ttttccggcc ccggttgtga aacaatgaag tcagcaaggg
    17041 cgaggtgacc aatctccgat gaatggcgtc aaaaccgtcg tactgctcgg gttgttgagc
    17101 gggttgctgc tggtgggtgg agaggccgtc gccggccgga atgggctcta catgggcctc
    17161 gcgctggctg tggtcatgaa tgctgccagt tacttcttct cggacaagat cgcgctcgct
    17221 atgtaccgtg cccagcccgt tacgcagacg gagaacggcc aggtctacgc acgggtggca
    17281 ccgatggttc aaaacttatg ccagcggatg gggctgccga tgcccaagct gtggctgatt
    17341 cccgagcagt ccccgaacgc cttcgccacc ggacgcaacc ccgcacatgc ttcggtagcc
    17401 ttcaccgccg ggctgctcga gttgatggac gtcagcgaac tggaaggcgt gatcgcgcac
    17461 gaactcggac acgttaagaa ccgcgacatc ctcatctcct cggtggcggc gaccattgcc
    17521 gcggccatca cgatggtggc ccgcatggcc atgttcgccg gcatgttcgg cgggcgccgc
    17581 gatgaccgcg atgatagcgg cggcggcctc ggagggttgt ttatgctgat cgtcgctccg
    17641 atcgcggcga tgctgatcca gatggccatc tcgcggacgc gtgagtactc ggccgacgct
    17701 accgcggcgc aatacaccgg cgggccctac ggactcatca gcggcctgcg gaaactcgaa
    17761 ggctggtcga aacggatccc catggatgcc tccccggcga ccgcgcacat gttcatcatc
    17821 aagccgttca gcggccaaac tctgatgcgg cttttctcca cccacccttc gaccgaagat
    17881 cgggtccgca ggctggaagc gctcgcgtaa ctgaggaccg gtacgccgcc caccgttcgt
    17941 atctctcgca agggtgcgga tcgcatcgcg tccggacacc cctgggtctt ttcgagcgac
    18001 gtgcaggatc gaggcggagc ttcgccgggc gacgtggtga cggtcgcaga tccgcgcggg
    18061 cggccgctcg gcaccgccca ctacagcgat acatcacaga tttccctacg cctgctcacc
    18121 gagcggttcg aacccgtcga ccgaagcttc tttgctgggc gtctgaaggc cgcctcggag
    18181 tttcggcaac gcgtcgttca cgacacctcc gcgtaccggc tcgttcacgg cgaagcggac
    18241 ggacttcccg ggctggtcgt cgaccgatac ggggatttct tggtcattca aacgcttgac
    18301 cagggcatgg accgttctct gcccgacatc gtagcctgtc tcgaagatct gtgcgcgcct
    18361 tcgggcattg tcgcgcgcaa cgacgcggcc gtccgcgtcc gcgagtcgct cccgagggaa
    18421 agtaccgttc tcaccggcaa cgttcccgac cgcgtatcag tgcgtctcaa cgggctgacc
    18481 ttcctggtgg atattctccg cggccagaaa acgggcttct ttctcgacca acgcgaaaat
    18541 tactccgcca cggcccgcta cgctcgtggc cacgcgcttg actgcttcac gttcgaaggc
    18601 ggtttcgcgc tgcacttggc cggcgggtgt gaatccgtgg aggccgtgga tagttcggaa
    18661 cacgcgctat gcctggcgcg agagaacgcc ggcttgaacg gaatcggcaa cgtgcagttc
    18721 cgccaggcgg atgtttttga cctcctggcc ggttactcgg cggcgggtcg ccgctttgat
    18781 accgtagtcc tggacccgcc ggggttcgcc aagtctcgct ccgccatcga aggcgcagcc
    18841 cgcggctaca aggacctgaa tcttcgtgct ttgcgcctgc tcggcgccgg cggtgtgctc
    18901 gtcacctgtt cctgctccca ccacgtgagc gaagcgatgt tgttggagat cgttgcctct
    18961 gcgtccctcg atgccgaccg gaagctccgt gtccttgaac gccgcaccca ggccctcgat
    19021 cacccgattt tgctgaccat ccccgaaacc cattacctta agtgcgttat tctggaggtt
    19081 gcgtaaacgg ctctccctgc cgcttgtgaa ccataactaa gttgattccg acatggcggt
    19141 ccgatatagt caaaagtggg ccgcaaacgt aaaggattgc tctattcgca gatctttcgc
    19201 ccgccggcga cgtggcccaa ctcgccggag ggtgccgtgt actcacaatc aggaggctct
    19261 ttgtaatgtt tcgcagtgaa gccctgcccg tcatccagcg gcaggcaagt tcccaaaagg
    19321 cgcttggccg gccagtgacc ttcggcctgt tgcgcacggt tctcgttctt ctctcacttg
    19381 ccgtcctcgc ggcgcccatg ttcgcccagg agcatcacgg cggcgaggcc aacctcattc
    19441 tgccggatct cgaccaggcg acttttctgg gtggaattgg tggtcggtcc ctgttaatgg
    19501 ttggcctgct ggtcagcggt ctcggcttgc ttttcggcct tgttatatac aggcggcttc
    19561 gcgacatgcc ggtgcacgcg gcgatgcgtg aggtctccga gctcatctac gagacctgca
    19621 agacctatct ggcgacgcaa ggcaagtttc tgatgatgct cgaagccctg attgccgtca
    19681 tcatcgtttt ctacttcggt ttcctgcagg gattcgaggc gtttcgcgtc ggcatcatcc
    19741 tgctcttcag cgtggtcggc atctccggca gcttcctg
//
LOCUS       DATIFQ010000061         3481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1169394, whole genome shotgun sequence.
ACCESSION   DATIFQ010000061 DATIFQ010000000
VERSION     DATIFQ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169394"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..576
                     /gene="rplU"
                     /locus_tag="VLE22_02700"
     CDS             265..576
                     /gene="rplU"
                     /locus_tag="VLE22_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSB13338.1"
                     /translation="MYAVIETGGKQYRVAPGETIDVEKLPGEVGSEIEFDKVLAVFKE
                     PEQLLAGSEVSQAKVKGTITGHGRGDKVLVFKFKRKKQYKRTIGHRQSYTRVQVNEIL
                     A"
     gene            587..844
                     /gene="rpmA"
                     /locus_tag="VLE22_02705"
     CDS             587..844
                     /gene="rpmA"
                     /locus_tag="VLE22_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HSB13339.1"
                     /translation="MAHKKGLGSSKNGRDSNAQRLGLKAFGGQMVPGGAIIVRQRGTK
                     FKPGDNVGRGKDDTLFAKVTGIVSFRDRGRLGKFISVSPTE"
     gene            923..1942
                     /gene="obgE"
                     /locus_tag="VLE22_02710"
     CDS             923..1942
                     /gene="obgE"
                     /locus_tag="VLE22_02710"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HSB13340.1"
                     /translation="MFIDEVRVFVKAGDGGNGCLAFRREKYVPRGGPSGGDGGRGGDI
                     ILVASQHHNTLLHFRYNPEHKAGRGRHGEGSNKTGRDGESVELPVPVGTVVYDADTGE
                     LLHDFTRPGENYMIARGGRGGRGNARFATSTHQAPTEHEPGRPGEEKRLRLELKLLAD
                     VGLVGFPNAGKSTLISRISAARPKIADYPFTTLEPHLGVVGLEDGRTFVVADIPGLIE
                     GAHLGQGLGTRFLRHVERTRLLAHLVDVSDASGRDPVHDFDVVLNELSSFSPELAAKP
                     MLVVAAKMDAAQEPERVESLRAMAARRGLPFYELSSVTGLGVERLKLAMGDAALQKSA
                     ETEQP"
     gene            complement(1966..2727)
                     /locus_tag="VLE22_02715"
     CDS             complement(1966..2727)
                     /locus_tag="VLE22_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSB13341.1"
                     /translation="MSKHILSIHAHPDDAEILAGGTLALLASGGHKITIVTMTPGDCG
                     SAELSAEEISSIRRKEAEHAAEVIGAAYICAEFRDYVIFPDDSARRRVTEILRCAKPD
                     LILTASPVDYMVDHEVTSQLVRDACFGAPLKNYVTQSPALASIPHLYFMDPIGGVGRD
                     GLPILPDFVVNVESAFEEKNMMLASHRSQREWLLKHHGIDDYMDTMKQWTRECGRRAG
                     VELGEGFRLYRGHPYPQTPLLEELLGEDLVSRRLT"
     gene            complement(2724..>3481)
                     /gene="proC"
                     /locus_tag="VLE22_02720"
     CDS             complement(2724..>3481)
                     /gene="proC"
                     /locus_tag="VLE22_02720"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HSB13342.1"
                     /translation="RKHVVATVRTAGRAREVSEKYSIPVTAGGNREAAEWSDILIVAV
                     KPPTLPRLLEKISGVLRSDQILISLAAAVPISLIEKTVDKRIPVFRAMPNIPVVVEEG
                     ATAVSANAAVTASHRKIVESIFRAVGVVCFVEEDQMHAVTALSGSGPAYIYMIIEALI
                     AGGLKMGISREISTRLAEQTVLGAAKLVRETGLHPAILKDQVITPGGVTISAIHELER
                     HGLRAMLISAIETATNHSRKLSEALLDRFSKES"
BASE COUNT          664 a          988 c         1193 g          636 t
ORIGIN      
        1 aggtcggaga gggcgttcac gaacgattcg tggttgacgt ggcgcggttc gcatcgaggg
       61 ttcaagcgaa actggcaggc cgctaggagc tgggcgagcg acagggaggc gcaacgcagg
      121 agttcctgga gcaatgggcg actccgggtt gttgacggaa gcggggtgtt gtaagataag
      181 agattgcagc tctcggcgcc gacccttatt tagcgggttg ccttcctgag tagcttccca
      241 aaacgacgac agacgaggat tgtcatgtac gcagtaatcg agactggcgg gaaacagtac
      301 cgcgttgctc cgggcgaaac gatcgacgtg gagaagttgc ccggcgaagt gggttccgag
      361 atcgaattcg acaaggttct ggccgttttc aaagagccgg aacagttgct ggcgggttcc
      421 gaggtgagcc aggcgaaggt gaaaggcacg atcacgggcc acgggcgcgg ggataaggtg
      481 ctggtgttca agttcaaacg gaagaagcag tataagcgga cgatcgggca ccggcagtcc
      541 tacacacgcg tgcaggtcaa cgagattcta gcgtaggagg gcagagatgg cacacaaaaa
      601 aggcctgggc agttccaaga atggccgcga ctcgaatgcg caacgtctcg gcctgaaagc
      661 gtttgggggc cagatggttc cgggcggggc catcatcgtg cggcagcgcg gtacgaagtt
      721 caaacccggc gacaatgtcg gccggggcaa ggatgacact ctgttcgcga aggtaacggg
      781 catcgtctcc ttccgcgatc gcgggcgcct agggaagttc atcagcgtca gccccaccga
      841 gtaagccgac tcgcagtccg cagcctgcgg ctctccggcc ggcagtcatg cgctgccgct
      901 agcatcacgc cacgagtcct ccatgttcat cgacgaagtc cgggtttttg tgaaagccgg
      961 tgacggcggg aacggctgtc tcgcgttccg gcgggagaaa tacgtcccgc ggggcggacc
     1021 aagcggcggg gacggcggcc gcggtgggga catcatcctt gtcgccagcc agcatcacaa
     1081 cactctcctt catttccggt acaatccaga gcacaaagcc ggccgcggcc ggcacggcga
     1141 gggaagcaac aagaccggaa gagacggcga atcggtcgaa ctgcccgttc cggttggaac
     1201 ggtcgtctac gacgcggaca cgggtgaact gctgcatgac ttcactcgtc cgggtgagaa
     1261 ttacatgatc gcccggggtg gtcgaggcgg gcgtggaaat gcgcgcttcg cgacttcgac
     1321 gcaccaagcg cccaccgagc acgaaccggg caggccgggc gaagaaaaac ggctgcgttt
     1381 ggagctgaag cttctcgccg atgtcggact ggtgggcttt ccgaacgcgg ggaaatcaac
     1441 cctcatttcg aggatttctg cggcacggcc gaagatcgcc gactatccgt tcacgaccct
     1501 cgagccacac cttggggtgg tgggtttgga ggacgggcgg acgttcgtgg tggcggatat
     1561 cccggggttg atcgagggtg cgcacctcgg gcagggtctg ggcacccggt tcctccgtca
     1621 cgtggagcgg acgcgcttgc tggcgcacct ggtcgacgtg tccgacgcga gcgggcggga
     1681 cccggttcac gacttcgatg tcgtgttgaa cgaactgtcg agcttcagcc ccgagctggc
     1741 ggcgaaaccg atgctggtgg tggcagccaa aatggacgcg gcacaggagc cggagcgcgt
     1801 ggaatcgctg cgcgcgatgg ccgcccgccg cgggttgccg ttctatgagc tctccagcgt
     1861 cacggggctc ggtgtcgagc gactgaaact ggcgatgggg gacgccgctc tgcaaaagtc
     1921 ggccgagacg gaacagcctt agcattcact agcgcccgaa ccggcttagg tcaaccggcg
     1981 ggacaccaga tcctcaccga gcaactcttc gagcagcggc gtttgcgggt aggggtggcc
     2041 cctatacagg cggaacccct cgccgagctc gacgccggca cgacggccgc actcacgcgt
     2101 ccattgcttc atggtgtcca tgtagtcgtc gatgccgtgg tgcttcagaa gccattcgcg
     2161 ctggctgcgg tggctggcga gcatcatgtt tttctcttcg aaggccgatt cgacgttcac
     2221 cacgaagtcg ggcaggatgg gaagcccgtc gcggccgacg ccgccgatcg ggtccatgaa
     2281 gtacaggtgc gggatcgagg ccaacgcggg ggattgggtg acgtagttct tgagcggcgc
     2341 tccgaaacat gcgtcccgaa caagctggct ggtcacttcg tgatccacca tgtagtccac
     2401 gggcgacgcg gtcaggatca ggtccggctt cgcacagcgc agaatttcgg tgacccggcg
     2461 gcgggcgctg tcgtccggga aaataacgta gtcgcggaac tcggcgcaga tgtaagcggc
     2521 tccaatcacc tccgcggcgt gttcggcctc tttccggcgg atcgaggaga tctcttcggc
     2581 ggaaagctcg gcggagccac aatcgcccgg ggtcatcgtc acaatggtga ttttgtgtcc
     2641 ccccgaggcc agcaatgcca gggtgccgcc ggccaggatt tcggcgtcgt caggatgtgc
     2701 atgaatggaa agaatgtgct tgctcatgat tccttgctga atctgtccag gagagcctct
     2761 gacagttttc gcgaatggtt cgttgcggtt tcgatggcgg agatcaacat tgcccgcaac
     2821 ccgtggcgtt cgagttcatg tatggcggag atggtcacgc cgccgggcgt gatgacctga
     2881 tccttcagga tcgcggggtg cagcccggtc tcgcgtacga gtttggccgc gccgagtacg
     2941 gtctgctctg ccagccgggt ggagatctca cgggagatgc ccatcttcag acctccggcg
     3001 atgagcgctt caatgatcat gtagatataa gccggtccgc tgccggagag cgccgtgacc
     3061 gcgtgcatct ggtcctcttc gacgaagcac accacgccga cggcgcgaaa gatgctttcg
     3121 acgatcttgc gatgggacgc ggtcactgcg gcgttcgccg agacggcagt ggctccttcc
     3181 tcaaccacga ccgggatatt cggcattgcg cgaaagacgg ggatgcgctt gtctacggtt
     3241 ttttcaatga gagaaatggg gacggcggcg gcgagtgaga tcaggatctg gtcgctgcgg
     3301 agcactccgc tgattttctc gagcaggcgc ggcaacgtcg gcggcttcac tgccacgatc
     3361 aggatgtcgg accattcggc cgcttcccgg ttgccgccgg cggtcaccgg gatcgaatac
     3421 ttctcggaga cctcacgggc gcggccggcg gtacggacgg tggccaccac gtgcttgcgg
     3481 t
//
LOCUS       DATIFQ010000062         5010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_211936, whole genome shotgun sequence.
ACCESSION   DATIFQ010000062 DATIFQ010000000
VERSION     DATIFQ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211936"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VLE22_02725"
     CDS             <1..1074
                     /locus_tag="VLE22_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB13343.1"
                     /translation="ILEYAVSNMAVAVGFSAYLNDFLESMLGFQLPKQLSAPMISGGQ
                     FTGSWFNLPALLVLLALTWILVRGVRESAETNNVMVLVKVGAILIFVIGAARAIEPGN
                     WRPFMPNGFQGVMTGAAIVFFTYIGFDSVSTAAEECKRPQRDLPFGIIATLIVCTLLY
                     AAVALVLTGIADYKTLANAAPVANALKAIGFENIRRWVSVGALMGMVSSLLVFQYGQA
                     RVWFAMSRDGLLPPAFSRVHPVHQTPYISTWVAGLAVGLPAGVWDIGTFADLANIGTL
                     FAFVLVSAGVIVLRRRQPERPRSFRVPLVPWLPMLSIASCFVLMLSLPLETWVRFFVW
                     LVIGLAVYFVFGKKHSELADRTW"
     gene            1049..1792
                     /locus_tag="VLE22_02730"
     CDS             1049..1792
                     /locus_tag="VLE22_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB13344.1"
                     /translation="MSWPIEPGNAVCGGVALVVCVVLCATARPQVADQHHPPRSAEEY
                     AKVLDAPSREAWQKPDEVVRALGFKPGESVADIGAGTGYFARRFAPYVAKVYAVDIDD
                     KLLKTAAEGAPPNLETVLATPADPKLPEASVDVIFFCNVLHHIENRTAYYQKLARALK
                     PGGRIVNIDFFKKPLPVGPPVGMKLSEDEVVAEFKAAGFSLVKTHSFLPYQYFQVFSR
                     ADRSSRFPASFRVAISAPDSRHLVNLKYD"
     gene            1978..2550
                     /locus_tag="VLE22_02735"
     CDS             1978..2550
                     /locus_tag="VLE22_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13345.1"
                     /translation="MTGTIVELEPTTRSGVIRSEDGSRVVFRGPAIGGEFDTLSVGGR
                     VRFDYDRTWSEVTAVHVLVETILPDAPLDLRYMGFDQAKDVRRYRFDAILGGKLKRRF
                     VVVADMALFRKHHIAVQDGPTLCLHKLMSGLETAPESSCYELGNDDLLAHAEARATAA
                     SRKPRPKGLTGRARNGAASHSPWGRPPESR"
     gene            2615..2803
                     /locus_tag="VLE22_02740"
     CDS             2615..2803
                     /locus_tag="VLE22_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13346.1"
                     /translation="MGTRSHTTYKKRQKELARQDKQREKAARRAQRKLEKQTIGSDHE
                     EGIEPVAVADVEQGEELR"
     gene            2800..3003
                     /locus_tag="VLE22_02745"
     CDS             2800..3003
                     /locus_tag="VLE22_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HSB13347.1"
                     /translation="MKEKGTVKWFNAAKGYGFIQRENGEDVFVHFSAIEASGYRSLDE
                     GAAVEFVVKKGPKGLQAESVALL"
     gene            complement(3175..4458)
                     /locus_tag="VLE22_02750"
     CDS             complement(3175..4458)
                     /locus_tag="VLE22_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB13348.1"
                     /translation="MKRRTLAIPCAALALVLANLTGCRTATKAGPRTVTVAQSGAADV
                     TGSDNVALQKAADMLRPGDALSIGPGTYTMENSVFIPTGVTVRGTKGQTILQKGKGVQ
                     SALAEDGDYGESQLLVAEPEKFRPGMGITVLDDQQNSGWDVSVTAVKAIEGNLLRIEP
                     MTLRDYSLEPHHARIQNTFPILAVIEGENVVLEDLIVDGNKDENARIDGCRGGAIYLY
                     RSKNSTVRNCVARNYNGDGISFQITDGIQVIDSEAYGNTGFGVHPGTGSPRATVKGCK
                     LHHNGDIGLFLCWRVRNGEFSGNIIEDNGHYGISIGHKDTDNVFTNNTVRRNGVCGVY
                     FRNETVKNAGHRNTFRNNLIADNGTVKQGYGFLIEPKAGDIVIENNRISDSRTEGRAQ
                     RYAVYKVAGAGEVKLASNTIEGNFAAEFKEGPASR"
     gene            4541..>5010
                     /locus_tag="VLE22_02755"
     CDS             4541..>5010
                     /locus_tag="VLE22_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HSB13349.1"
                     /translation="MHLRLSAILGALILTGMPAVPRQISIIPQPATVTGMEGEFILKP
                     GTVVVTDNASAAVARRFTADIAAATGYKLRIAEAGQARDNSITLGIDDSLGKLGAEGY
                     QLEVTSKRVTVRAPKPAGVFYGAQSIRQLLPPAVYGKASSGVTWSMPCVRIEDYP"
BASE COUNT          917 a         1482 c         1577 g         1034 t
ORIGIN      
        1 atcctcgaat acgcagtctc caacatggcc gtagccgtgg gcttttcagc ctatctcaac
       61 gacttcctcg aaagcatgct cggctttcag ttgcccaaac agctctccgc tccgatgatc
      121 tcgggcgggc agttcactgg aagctggttc aaccttccgg cactgttggt gctgctggcg
      181 ctcacctgga tcctcgtgcg cggggtgcga gaaagcgccg aaacgaacaa cgtcatggtg
      241 ctggtgaagg tgggcgcgat cttgattttc gtgatcggcg cggcccgcgc catcgagccc
      301 gggaactggc ggccgttcat gccaaatggg tttcagggcg tgatgacggg cgctgccatc
      361 gtttttttca cctatatagg cttcgattcg gtctccaccg ccgctgaaga gtgcaagagg
      421 ccgcagcgcg acctgccgtt cggcatcatc gccacattga tcgtttgcac gctgctctac
      481 gccgcggtag cgctcgtgct taccggtatc gccgactaca agacgctcgc gaacgcggct
      541 ccggtggcca acgcgctcaa ggcgatcggc ttcgagaaca ttcggcgttg ggtcagcgtc
      601 ggcgcgctga tggggatggt gtcttcgttg ctcgtattcc agtacggcca agcgcgggtg
      661 tggttcgcca tgtcccgtga cgggctgctg ccgccagcgt tttcgagggt gcatccggtt
      721 caccagacgc cgtacatcag cacctgggtc gccggacttg cggtgggact tccggcaggc
      781 gtctgggaca ttggcacgtt cgccgacctg gcgaacatcg gcaccctgtt cgccttcgtg
      841 ctcgtatcgg ctggtgtgat cgtactgcgc cgccgccaac cggaacgccc gcgcagcttt
      901 cgggtgccgc tggtgccctg gttgccgatg ttgtctatcg cgagctgttt cgtgctgatg
      961 cttagcctgc cgctcgagac gtgggtacga ttctttgtct ggctggtcat cgggctggcc
     1021 gtgtacttcg tgttcggaaa gaaacatagt gagttggccg atcgaacctg gtaacgctgt
     1081 ctgcggcggt gtggcgctgg ttgtctgcgt agtgctgtgc gcgacggcca ggccgcaggt
     1141 ggccgatcag caccacccgc ctcgttcggc cgaggagtac gcgaaggttt tggacgcgcc
     1201 gtcacgcgag gcgtggcaga agccggatga ggtggtaaga gcgctaggat tcaagccggg
     1261 cgagtcagtc gccgacattg gggccgggac aggctacttt gcccgccggt tcgcgccgta
     1321 cgtggcgaag gtctacgcgg tcgacattga tgacaagctg cttaagaccg ccgcagaggg
     1381 cgcgccgcca aacctggaga cagttcttgc gaccccggcc gacccgaaac taccggaggc
     1441 gtcggtagac gtcatcttct tctgcaatgt gctccaccac atcgagaatc gcaccgccta
     1501 ttaccagaag cttgcacgcg ctctgaagcc gggcggtcgc attgtgaaca tcgatttttt
     1561 caagaagcct ctgccagtcg gccctccggt cggcatgaag ttgtccgagg acgaggtcgt
     1621 cgccgaattc aaggccgccg gcttcagcct tgtcaagaca cactcgttcc tgccgtatca
     1681 atacttccag gtcttttcgc gcgcggatag atcaagccga tttccagctt cttttcgcgt
     1741 ggccatcagc gcgccagaca gccggcatct ggtaaacttg aagtacgatt gatcgtccag
     1801 cctagcttgg ccgcgataag tccaggctcg taaagccgct ttcaatccga agggagaccg
     1861 cgtgaaaaac atctctgcgg gcgatctcga ttccggccgc cacaaagttc cacacttgcg
     1921 gcccgttgtg tcgctccggg acatcgggac cttctcatcc gacgggagaa aagggctgtg
     1981 acgggaacga tcgttgagct ggagcccaca actcgcagcg gagttatccg ttccgaggat
     2041 gggtctcgcg tggtgttccg tggacccgcg attggcggcg agttcgatac gctgtcggta
     2101 ggggggcgag tccgattcga ttacgaccgg acgtggtccg aggtgacagc cgtccacgtt
     2161 ctggtggaga cgattcttcc ggatgcgccg ctggatctcc gttacatggg attcgaccag
     2221 gctaaggacg tccgccgata caggttcgac gccatcctgg ggggcaaact caagcggcgt
     2281 ttcgttgttg tggctgacat ggcgctgttc cggaagcatc atatcgccgt ccaggacggg
     2341 ccaaccctgt gcctgcacaa gctgatgtcg ggtctggaga ccgctcctga atccagctgc
     2401 tacgaactcg gaaacgacga tctgctcgcg catgcggagg cccgggccac cgccgcttcg
     2461 cgcaagccca ggcccaaagg gctgaccggg cgagcccgga acggggcagc ctctcacagt
     2521 ccgtggggcc ggccaccgga atcacggtga accgtgttca agacgaacga cgcgccgcga
     2581 gtgaggcttg attgcggcga ggaaaggaga gtggatggga actcgttcgc atactacata
     2641 caagaagcgc cagaaagaac tggcaaggca ggacaagcag cgcgagaaag ccgccagacg
     2701 ggcgcagcgc aaattggaga agcagactat cggatccgac cacgaagaag gcatagagcc
     2761 agtagcggtc gctgatgtcg aacaagggga agaattgaga tgaaagaaaa aggaacagtg
     2821 aagtggttta acgccgcgaa gggctatggc ttcattcaga gagagaacgg agaagatgtg
     2881 tttgttcatt tctccgcgat tgaggcgagt ggatatcgct cgctcgacga gggtgccgcg
     2941 gttgagttcg tcgtgaagaa gggccccaaa ggccttcagg cggagagtgt agcgcttctg
     3001 tagctgctag cgaaccacgg ccggctgggc aaaagtgccc gggcggtaca gaagacaaaa
     3061 gaaaccacgg ataggaaaaa cggggccagg cagcaccgtc cacgcggaaa tcatgcctcg
     3121 gacaacgctg ccaggctccg ttctttcgat ttcatcgatg ggcccagccc gccgtcaccg
     3181 gcttgcgggg ccttccttga attcggcggc gaagtttccc tcgatcgtgt tactcgccag
     3241 cttcacttcc cctgcgccgg ccaccttgta gacagcgtac cgctgcgcgc gcccctcggt
     3301 gcggctgtcg gagatccggt tgttttcgat cacgatgtcg ccggcttttg gttcaatcag
     3361 gaaaccatag ccctgcttga ccgtgccgtt gtcggcgatc aggttgttgc gaaacgtgtt
     3421 ccggtggccg gcgttcttaa ccgtctcgtt ccggaaatag acgccgcaga cgccgtttcg
     3481 tcgcacagta ttgttcgtga agacgttgtc ggtgtccttg tggccgatcg agatgccgta
     3541 atgtccgttg tcctcgatga tgttgccgga gaactcgccg ttccggacgc gccagcatag
     3601 gaaaagcccg atgtccccgt tgtggtgaag cttgcatccc ttgaccgttg cacgcgggct
     3661 gcccgtcccg ggatgcacac cgaatccggt attcccgtac gcctcagagt cgatgacctg
     3721 aattccgtcg gtgatctgga aggagatgcc gtcgccgttg taattcctcg ccacacagtt
     3781 ccggacggtg gagttctttg aacggtacag gtagattgcg ccgccgcggc agccgtcgat
     3841 ccgggcattc tcgtccttgt tcccatccac gatcaggtct tccaggacga cgttctcgcc
     3901 ttcaatcacg gcgaggatcg ggaaagtgtt ttggatccgc gcgtgatgtg gctcgaggct
     3961 atagtcgcgg agcgtcattg gttcgatccg cagcaggttg ccctcgatcg ccttgacagc
     4021 ggtgacgctg acgtcccagc cgctgttctg ctggtcgtcg aggacggtaa tccccattcc
     4081 ggggcggaac ttctccggct ccgccacgag aagttgcgac tcgccgtagt cgccatcttc
     4141 ggcgagcgcg ctctggactc ctttgccttt ttgcaagatg gtctggccct tggtgccgcg
     4201 taccgtgaca ccggtaggaa tgaatacgct gttttccatc gtgtacgtgc ccgggccgat
     4261 ggacagggcg tctcctgggc gcagcatgtc cgcggctttc tgcaaggcca cgttgtcaga
     4321 gccggtaacg tccgccgcgc cggattgcgc cacggttacg gtacgaggcc cggccttcgt
     4381 ggctgtccgg cagccggtca ggttcgccag aacgagcgcc aatgcggcgc aggggatcgc
     4441 aagggttctc cgcttcatac gccgcacatt ttacaacgaa tggcccttcc acaaagacac
     4501 acgccgattc gatacactcg acgcggaggc ttcttttttc gtgcacttgc gactttcagc
     4561 catactgggc gcgttgatcc tgaccgggat gccggcggta ccccggcaga tttccatcat
     4621 tccgcagccg gccacggtga ccgggatgga aggagagttc atcctgaagc ccggcaccgt
     4681 ggtggtgacg gacaatgcgt cggcggcggt cgcccggcgc ttcacggccg acatcgcagc
     4741 cgccaccggc tacaagctga ggattgccga agccggccag gcgcgggaca acagcatcac
     4801 gctggggatc gatgactcgc tcggcaaact tggcgccgag ggctatcagc tggaagtcac
     4861 ctctaagcgc gtgacggttc gcgccccgaa gcccgccggc gttttctacg gggctcagtc
     4921 gattcgccag ttgcttcctc ccgccgttta cggaaaagct tccagcggcg tgacctggag
     4981 catgccgtgc gtccggatcg aggattatcc
//
LOCUS       DATIFQ010000063         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_20336, whole genome shotgun sequence.
ACCESSION   DATIFQ010000063 DATIFQ010000000
VERSION     DATIFQ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20336"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..568)
                     /locus_tag="VLE22_02760"
     CDS             complement(245..568)
                     /locus_tag="VLE22_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13350.1"
                     /translation="MNRVPADAVAEFARLFTRRAELLRELAELEEALGRAFQSALSPE
                     PSDEVLGVAEAAAFMGEPVETFRRRFEYRKALLTRPRERRLRYSRVELERIRQNRLAA
                     RAVER"
     gene            complement(569..1120)
                     /locus_tag="VLE22_02765"
     CDS             complement(569..1120)
                     /locus_tag="VLE22_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13351.1"
                     /translation="MFERDTTRRALRFEAFLAIFILADVSQLRGQPADSEAQDWKIFV
                     HGRFDSGMTTAVNSAVKDARRKLENRECQLVFSDFRDASGRTIQENLDARKETAAGYL
                     RWLIFRNSSDQQYCMRSRSLAGTNPGDRVIQVCGQFQRVQMNDPGFAAASIIHEELHS
                     LGLPENPPSSFEINSRVMERCGR"
     gene            complement(1422..>2084)
                     /locus_tag="VLE22_02770"
     CDS             complement(1422..>2084)
                     /locus_tag="VLE22_02770"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HSB13352.1"
                     /translation="YAREYNEVKQVGDALVRGAAPDSEESDIARFWPGGGSNWNLTTR
                     VIVAGRGLDLWENARLFALLNMAETDGHIANQKNKYTYKFWRPVTAIRWADDGNPDTM
                     SDPGWRPFLVTPPYPDYPCALPTVSGAHTEVLRRFFGTDDIAFVRTHNAGAVPLPSPL
                     TALPPKAITRHFDSLSEAASESADARVFAGIHFRSGCTAGVRLGTQVGRFVIKHYLKP
                     LK"
BASE COUNT          371 a          637 c          687 g          389 t
ORIGIN      
        1 aacgatgtct cggatgattc tccgcccgtc tttggagcga ggtcaatctc gccttgcgtg
       61 catccatgcg aagccccgcc cggcgggtat ttggccggcg ggggacggcg ctaagtccta
      121 attacccgga caaatactga acgggttcga gtcccaggcg gccaaccact tcccaccact
      181 tgccaccggc ggactgactg agaaggatgg ccggaaacgg gagctggcgg tagggagggg
      241 cgctctatcg ttcgacggcg cgcgcggcga gccgattctg ccggatacgt tcgagctcga
      301 cacgactgta gcgcaaccgc cgttctctcg gccgcgtcag aagtgctttg cgatattcga
      361 atcgtcggcg gaaagtttcc accggctcgc ccatgaacgc cgccgcttcc gccaccccga
      421 gcacttcatc ggagggctcg ggggatagcg cactctgaaa ggcgcggccg agtgcctcct
      481 caagttctgc aagttcccgg agcagctcgg cccggcgggt gaagagacgc gcgaattcag
      541 ccacggcgtc ggccggcacc cgattcaatc agcggccaca gcgttccatg acacgcgagt
      601 tgatctcgaa gctcgacggg ggattctccg gcaggccgag ggaatggagc tcctcatgga
      661 tgatcgaagc cgccgcgaat ccgggatcgt tcatctgtac gcgttgaaac tggccgcaaa
      721 cctggatgac ccgatctccg gggttcgtcc ccgccagact gcggctgcgc atgcagtact
      781 gctgatcgga gctgtttcgg aagatcagcc atcggagata gcccgcggcg gtttctttcc
      841 gcgcgtccag gttctcctgg atcgtgcggc ccgaggcgtc gcgaaagtcg gagaagacca
      901 gctgacattc acggttttcg agcttgcgcc tggcatcttt gaccgcgctg ttgaccgccg
      961 tggtcattcc tgaatcgaag cgcccgtgga cgaagatctt ccaatcctgc gcctccgagt
     1021 cggcgggctg tccgcgaagc tgactcacat cggccagaat gaagatcgcc aggaaggcct
     1081 cgaaccggag agctctgcgg gtcgtatcgc gttcgaacat gagcggctcc tttcggagcg
     1141 ccccgccgct cctggaagcg gcgtgttggc gcgataccta ggattaggat tgggaaggcg
     1201 gcggtctcta tgagtgggtc cggatttcgt tcgctttttg tcggtgagaa atcctgcggc
     1261 tcgattttgg cgactgcagc aaatcggagg cgaatcccgg caaaatccgc gcgagcaacg
     1321 cctcattcgc cgcggctgcg aatctggcag caggcgatag agacagggtg gagtcgatcg
     1381 gtgatggaac gggccgtgtc gacccgttcc gcacgtctcg gttacttcag cggcttcaaa
     1441 tagtgtttga tgacgaatcg gccgacctgc gtgccgagcc gcacgccagc cgtgcagccg
     1501 ctgcggaagt ggattccggc gaacacgcgg gcatcggccg actcggacgc tgcctcggaa
     1561 agtgaatcaa agtgacgcgt gatcgccttg ggcggcagcg cagtcagagg cgacggcagc
     1621 gggactgcgc ccgcgttgtg cgtccggacg aaggcgatgt cgtcggtgcc gaagaaccgc
     1681 cgcaacacct ccgtgtgcgc gccgctgacc gtcggcaggg cgcagggata gtccggatac
     1741 ggcggggtga ccaggaacgg acgccagccc gggtcgctca tcgtgtccgg attgccatcg
     1801 tcggcccaac ggatggcggt gaccggccgc cagaacttgt acgtgtactt gtttttctga
     1861 ttggcgatgt gtccgtccgt ctcggccatg ttcaacaagg cgaacaggcg cgcgttctcc
     1921 cacagatcga ggccgcggcc ggcgacgatg acgcgcgtgg tcaggttcca gttggaaccg
     1981 ccgcccggcc agaaccgggc gatgtcgctc tcttcggagt cgggtgcggc gccgcgtacc
     2041 agtgcatcac cgacctgttt gacctcgttg tactcgcgcg cata
//
LOCUS       DATIFQ010000064        19092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2510819, whole genome shotgun sequence.
ACCESSION   DATIFQ010000064 DATIFQ010000000
VERSION     DATIFQ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19092
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510819"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VLE22_02775"
     CDS             complement(<1..915)
                     /locus_tag="VLE22_02775"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSB13353.1"
                     /translation="MWTRQRADAVDTRIVYWAYAALAWFAGFLLIAWGPMWLGGGPAA
                     ELSIRASLIRVLGSMLVAAGCFSAALATVPDPGSRRRGLLWIAAGHAVILVVVLLQQD
                     AVWSPLPAELPAAVLNCILLVFLYLWRTAESDSVTPFSPLTSLFGRANRSHTDRLRSQ
                     YEEQIRQAASREERSRLARELHDSIKQQIFVVQTAAATAQVRFDQDSAGARAALDEVR
                     NSAREAMAEMEAMLDQLRATPLENTGLVEALKRQCGALGFRTGAQIDFRIGKLPLNGS
                     LPPGAHQAVFRVAQEALANIGRHARAQNV"
     gene            complement(1019..1270)
                     /locus_tag="VLE22_02780"
     CDS             complement(1019..1270)
                     /locus_tag="VLE22_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13354.1"
                     /translation="MLQVVSMVWGILAILGMIVAFLPCLGSLNWLNIPFSVVGLVVSI
                     VALATANDGNRTGATVGVVCCGVATVFGIFRLAIGGGIL"
     gene            1684..2661
                     /locus_tag="VLE22_02785"
     CDS             1684..2661
                     /locus_tag="VLE22_02785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB13355.1"
                     /translation="MLSIVIPIHNEEPSILPLYDRLTAVLEKIHRPYEIIFIDDASSD
                     RSFDLLANLVETDGRLKVIRLRRNFGQTAALAAGFHEAEGDVIISMDGDLQHDPDDLP
                     ALLGKIDEGYDIASGWRKQRVDNAVTRKIPSRIANWLMAKASGVDLHDFGTTYKAYRA
                     EVLKDINLYGELHRFIPALASFYGARVAEVPIRNTPRATGGSHYGLSRTFRVLFDIIT
                     IKFLLKYFTRPMHFFGKLGLAGTTVGGSILAYLFVHKLMGGHIMTEHGPLMVLGGLLL
                     LAGLMMFTTGLLGEVLIRTYFESQGRRIYAVREIRTRRKPPVKEKDRPA"
     gene            complement(2722..3540)
                     /locus_tag="VLE22_02790"
     CDS             complement(2722..3540)
                     /locus_tag="VLE22_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB13356.1"
                     /translation="MNEELPAIDFQRFWRRLQQIGLNAYEARSYLVLVGHPRFKALEL
                     AARAHVPRQKIYEVLDSLVEKGFAQVVQEKTKLFSAVEPSLAIPGYLARKRDQLEHEL
                     TEQGRMAAGLTEDLNTAYCEVKGGRGTLDFLQIVSEPTQTATQYRRMLAEVQREYLEF
                     SRPPYAVDPLDEQLVREARGRGVACRLLLEAGTLSEDHRQRLGEYQAAGVEVRQAASL
                     PLKLALFDSSQGLIALLDPVITKPTWTSVVFNHPGLGEAMRGLFEGHWNRAQNF"
     gene            3649..5142
                     /locus_tag="VLE22_02795"
     CDS             3649..5142
                     /locus_tag="VLE22_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13357.1"
                     /translation="MTAHWDLPHTLGALRRSEFSDERIGRRSVKDELRENLIERLGTG
                     ETLFPGVVGYDDTVIPQIVNALLSRQNFVLLGLRGQAKTRILRMLTGLLDPATPCIAG
                     CEIHDSPYRPICRRCRNLVEEMGEETPIAWMKPEQRYVEKLATPDVTIADMLGDIDPI
                     KAARQGHDISDELTVHYGLLPRANRGIFAVNELPDLAGKIQVGLFNIMQEGDVQIKGY
                     PVRLPLDLLLVFTANPEDYTARGKIITPLKDRIGSEIRTHYPLSVEDGLAITRQEAWV
                     QRPATVKVEVPAYIAEVVEQLAFMAREDKRIDKRSGVSQRLPISALENVVSNAERRAL
                     RSGEDLAVPRVLDIYAALPAITGKLELEYEGELKGGETVARELVRMSVAKVYTTYFDG
                     ANVSAIVQWFELGGTLKLDELASSGSQVTQLGSIQGLMDRIGLLGIRPGAEDAERASA
                     AEFILEGLYAHRRISRTEERGFFAEEKKREPIPPEPGRRPGNRRQYN"
     gene            5156..6394
                     /locus_tag="VLE22_02800"
     CDS             5156..6394
                     /locus_tag="VLE22_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13358.1"
                     /translation="MRSIRYSRYTGEDFGISAEDLLNALSDFFLRSGFQHDPYSFQEW
                     NESSLENLKEAIRRALEQGMLSNDDRFEQMMRALESMTPEQLDQLLERLAQKLADEGY
                     INIEPQPEAPRAGTLGDGQNEQRIRVEVTDKTLDFLGYKTLKDLLGSLGKSSFGRHDT
                     RELATGVEASGAAKRYEFGDTLNLDVCATLFSAIRREGPGVPLALEYEDLHVHQAEYQ
                     SSCATVLMLDCSHSMILYGEDRFTPAKKVALALAHLIRTQYPGDSLRCVLFHDSAEEL
                     RLSELARVQVGPYYTNTRDGLILAQRLLRQEKKDMKQIVMITDGKPSALTLDDGRMYR
                     NAFGLDPLVISRTLEEVSRCKKQGILINTFMLASDYGLVDFVQKVTALCRGKAYFTTP
                     YTLGEYLLLDYMNRKTRTIH"
     gene            6456..7280
                     /locus_tag="VLE22_02805"
     CDS             6456..7280
                     /locus_tag="VLE22_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13359.1"
                     /translation="MKATLITAVALAALSLGQAQPVIDVGGVVNNASYRKAGTPGYGI
                     AQGSLFAVFGERLGPTNLFVAGAWPLGESLAGTSVEVTVGEVTLKAIPVYTSARQVGA
                     VLRSHTPAGEGTIVVRYDGETSAPASIRIVPRAFGIFTQNSAGHGPANAQQVRADARR
                     PMNSASDPARPGELVCLWGTGLGPVSGDEASGPRPGDMAVEFKVLVGDRPAEVVYRGR
                     SGCCAGIDEVYFHVPEGVKDCAAPVVIRSGGHTSNLATLAVADEHGVCGNPAFRAP"
     gene            complement(7319..7747)
                     /locus_tag="VLE22_02810"
     CDS             complement(7319..7747)
                     /locus_tag="VLE22_02810"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HSB13360.1"
                     /translation="MNETHVRRYFDAWNSGNEAALDELIAANCVHHDPANPDISTGPQ
                     GQKQLMRKYRTAFPDTRFRVEQIVEQGDMVATRWTVTGTHKGELEGVAATGKSVTVTG
                     ISLDRISGGKIVEGWSNWDALGLLTQLGVVRKPAAAGARA"
     gene            complement(7858..8622)
                     /locus_tag="VLE22_02815"
     CDS             complement(7858..8622)
                     /locus_tag="VLE22_02815"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSB13361.1"
                     /translation="MRRIVTALVLIPTIAYVALLAPQWLFLAVVAIVALLCFHEYGSI
                     VARHGFDPPGPAGYAAGILVLLVRDHELLLVTLLVLLALTLTLRTRDLSKALPGAAAF
                     LLGIVYVFGCWRFTIGLRQISPHWLFFTLALNWIGDSAAYYFGSAFGRHKLAPRLSPA
                     KSWEGSAASVVASALFGYFYLGWFLPSVPPLQVILVAVAANVAGQVGDLAESALKRGA
                     GVKDSSNLLPGHGGWLDRVDSTLFAIPVVYGLLQLL"
     gene            complement(8619..9452)
                     /locus_tag="VLE22_02820"
     CDS             complement(8619..9452)
                     /locus_tag="VLE22_02820"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HSB13362.1"
                     /translation="MKALFEVLRPGQKDWSLAEAIDPNRLPAHIAVIMDGNGRWAKRR
                     SLPRVAGHKAGVEPVRVTVETCARLGIRALTLYAFSVENWKRPRAEVDTLWRLLRIYL
                     RRELPNLRRNDIRLAAIGRVQALPAQAREELAAAVQATSLNRGLQVNLAINYGGRNEV
                     VDAVNAVLDEARRKGRLDSLTVDDNAIASHLYTAGLPDPDLLIRTSGEMRISNFLLWQ
                     IAYAELYVTDTLWPDFTRTELLRAILDYQKRDRRFGGLSPAGSSPGRESHLVGVGATA
                     R"
     gene            9510..10850
                     /locus_tag="VLE22_02825"
     CDS             9510..10850
                     /locus_tag="VLE22_02825"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSB13363.1"
                     /translation="MIRRGLPAVFLLFFLASALSPQNPPDLGAFLKAEMQRSKPDYVV
                     YVPGSVDGSTFDTGNEHFLVFDAPDGSLMAIWTQSTFEGAGDHRIMFTRSTDEGETWS
                     TPKRLVGPARKGEGPMASWGFPLTSKSGRIYVVYNQQQETADVHPQITGTMDCIFSDD
                     LGTTWSRPETIPMPRSCFDSPDASIASNWIVWQKPIRDLKDRWFNGFTRWVSKAVRTP
                     PHTNFSSHESVVEFMRFENIDANPRPPEIRVTYTPDTQALRVPHYTNPLLSIAQEPSL
                     LRLPDKRLFCVMRSMTGYIWYSVSGDDGQRWTNPRPLLRRDHGLPIRQPLSCTPMYEI
                     SAGRYFLLHHDNDGRFQGTKPEDTSWNRRPCYLVLGEFRKGADQPVWFSESKLLMDNK
                     GAQLGPLKRIDIGLYTSFTNRCGNAVLWHPERKFFLLGKRITSDFLADLKVPER"
     gene            10881..11738
                     /locus_tag="VLE22_02830"
     CDS             10881..11738
                     /locus_tag="VLE22_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB13364.1"
                     /translation="MSERAGGTYEFGPFRYDANQRLLFREGEIVPLAPKSLEMLQALL
                     ERRGMVVGKAELMKLVWPDTFVEEVGLARNISLLRKVLEDESEANTYIETVPRRGYRF
                     IAEVIRTGDWEQSSAPRQTRRLWWRVILAVVLVGLAGIIYWQFYAPSRYLPEGFASLA
                     VSPFETLTPDAEADAFSQGLTEVLAAELSKLKGVHVISPSTVRRYWRFRISAAMMARL
                     LGVQVIVEGTVQRSADRLMVTAQLTDVHSGKLIWAENFAVAAADPVAGRMTIARAVAE
                     QVGARLAVR"
     gene            complement(11917..12726)
                     /locus_tag="VLE22_02835"
     CDS             complement(11917..12726)
                     /locus_tag="VLE22_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HSB13365.1"
                     /translation="MIGAVIAFFAGLLIGSFLNVCIYRLPKDLSIVRPRSYCPACNVK
                     IAWFDNIPLVSYTLLGGRCRHCGVRIPFRYPIVEALTALAFALSVLALGPTLPALKVC
                     LFSALMIGLAFSDLEERILPDEFTLGGTAAGLLLALVVPMDVVFAGLFLNPLTTGRWL
                     WLAESILGAGVAAGLLWGVGFLYEKVRHKSGLGLGDVKMMGAIGAFLGVHGAFQTIIL
                     ASLVGSVVGLVYIKVSKKDFSSYELPFGTFLGLTALALAFLRATMPPLAGE"
     gene            complement(12723..13616)
                     /locus_tag="VLE22_02840"
     CDS             complement(12723..13616)
                     /locus_tag="VLE22_02840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB13366.1"
                     /translation="MRVLHVDTGSEMKGGQWQVLRLSEGLLSSQWEPVLAAPEDSELA
                     VRARASGIRVEPSRILSLRRLASDVDTVHVHDGRSHALSAALGLKPLVVARRVAFPVR
                     RSAVSRWKYRRADHYIAISNCVRDRLIEAGISDAKVSVVHDGVPVREPSGQGSLVVAP
                     ATRDPMKGRSLVEDAAAMAGVGVHFSENLEGDLRRAALFVYITREEGLGSAALLAMAA
                     AIPVVASRVGGLPEAVRDGETGLLVENRPAAIAEAIHRLLQDEELRRRLGERARQVVQ
                     EEFSVEKMVRETVKIYEWVLR"
     gene            complement(13618..14355)
                     /locus_tag="VLE22_02845"
     CDS             complement(13618..14355)
                     /locus_tag="VLE22_02845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB13367.1"
                     /translation="MKISATIITLNEEDNIARAIESLRCCDEVVVVDSGSQDRTMEIA
                     AKLGARIVESPWRGYAGQKNFAAEKASYDWILSIDGDEALSEALEGEIWQIKKSGPKF
                     DAYTMPRMAQYLGRWILHSGWYPDRKVRLYDRRRAQWAGDFVHESVNVSGSVGHLDYN
                     LLHFTCDSISSHLKRMERYTTLAAQDLVARGKKVTLADLLLDPPWTFLRTYVFQAGFL
                     DGIEGLTIAYMAAFYNFVKYAKARNMS"
     gene            complement(14367..15233)
                     /locus_tag="VLE22_02850"
     CDS             complement(14367..15233)
                     /locus_tag="VLE22_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSB13368.1"
                     /translation="MHVLLFLLTLVTTSAVGARLAHNFRYNRPAIDVSQDLESYIRLF
                     TDPRAWIDGLPFSLTLLLILLAHELGHYSACVHYRLDASLPYFLPSPTVIGTLGAFIR
                     IRAPIYSKRVLFDVGIAGPIAGFVFVLPALAIGLAFSKVVPGLGARGDLIFGTPAIFH
                     VFQWAIFPGVPAQDISLHPVARAAWVGVFATALNLLPIGQLDGGHILYAFFGERHRLL
                     SRIFVASLVPMGLVYWPWLLWAAILFFFGMRHPMVYDHSELGPIRKRLGVLALALLVL
                     SFMVAPIETSGG"
     gene            complement(15354..15620)
                     /locus_tag="VLE22_02855"
     CDS             complement(15354..15620)
                     /locus_tag="VLE22_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSB13369.1"
                     /translation="MIKLDIINEVVNRTGITKTKAEMAVETVFESMKRALGRGERIEL
                     RGFGIFNVRPRKTGIGRNPRTGAEVAIPPGKAVRFKPGKELQSL"
     gene            15742..16224
                     /locus_tag="VLE22_02860"
     CDS             15742..16224
                     /locus_tag="VLE22_02860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HSB13370.1"
                     /translation="MKLPSANKDQFRRACSKFATGITVASVIGVDGSPHGLTVNSFTS
                     VSLTPPLVLFCIDLGSGVLRHFRPARFFGINVLDQHQRSVSSHFARKGHDRFTGIAWL
                     PGKTGVPLLPGAIATLECRTVRRFAAGDHLVVIGEAVTAAAHEGKPLLYFASGYHEIP
                     "
     gene            complement(16287..16796)
                     /gene="tpx"
                     /locus_tag="VLE22_02865"
     CDS             complement(16287..16796)
                     /gene="tpx"
                     /locus_tag="VLE22_02865"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HSB13371.1"
                     /translation="MERAGATTLKGNPLTLIGPALKPGDKAPDFEVVDTTLSAVNLAK
                     TGNSVRIFSVVPSLDTPVCDAQTKRFNDELAKLPNVEVYTISMDLPFAQKRWCGAFGV
                     DRVKMVSDHKTGSFGSAYGTLIKELRIESRAIFVVDKNNVIRHVEYVKEVGDHPNYDT
                     ALAAAKAAL"
     gene            complement(16840..18948)
                     /locus_tag="VLE22_02870"
     CDS             complement(16840..18948)
                     /locus_tag="VLE22_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSB13372.1"
                     /translation="MHTNRLAQEKSPYLLQHAHHPVDWHAWGEEAFDVARREDKPIFL
                     SVGYSTCHWCHVMERESFEDDRIAEILNRDFVPVKVDREERPDVDRIYMLFVQVTTGS
                     GGWPMSVWLTPDLKPFFGGTYFPPDTRYGRPGFAFVLERIVEAWRADRARLVESGAAI
                     VEELRKQSEVTPSAAFLNDGPLESGYHAFRRIFDPNLGGFGGAPKFPRPSVHNFLLRY
                     FARSGNHEALEMVLKTLQAMARGGMNDQLGGGFHRYSVDDRWFVPHFEKMLYDQAQLA
                     TSYLEAFQITGQASCAAVARNVLEYVLRDMTHPEGGFYSAEDADSVIDPAQPDVKGEG
                     AFYVWSQDEIAARLRSPAAEWFCYAYGVQETGNVAEDPQDEFTGKNILYEARTATEVA
                     ARFGAGVDEVLASLAASRNVLLEARSKRVRPHLDDKILTAWNGLMISAFALGAQVLDE
                     PRYLAAARRAADFILGRLFDGHTGVLMRRYRDDEAAISGFLDDYAFFTQALVDLYETC
                     FELRYLETAERLAEEIGEWFEDPEHGGFFSTADNDASLLLRMKDDYDGAEPSGNSIAA
                     LNLLRLAQMTGRDDFRVAAEKTLLVFASRLQAMPAGIPQMLVTLDHYLAKQRQIVVVG
                     ARDAVDALALLQAVRRRFDPNKVVLLVHSEDVREALSRNVPAIAGMRRVGGRAAAYVC
                     EDFTCKLPVTEPEQLSELLK"
BASE COUNT         3831 a         5823 c         6037 g         3401 t
ORIGIN      
        1 tacgttttga gcgcgtgcgt gtcggccgat atttgccagc gcttcctgcg cgacgcggaa
       61 gaccgcctgg tgagcgccgg ggggcaagct cccgttgaga ggaagcttgc cgatccggaa
      121 atcgatttgc gcgccggtgc ggaaaccgag cgctccgcac tggcgtttca gggcttcgac
      181 caggccggtg ttctccagcg gagtcgcgcg aagctgatcg agcatcgcct ccatctcggc
      241 catcgcctcg cgcgccgagt tgcggacttc atccagcgca gctctggcgc cggcggagtc
      301 ttggtcaaat cgcacctgcg cggttgcggc cgcagtctgc actacgaaga tctgctgctt
      361 gatggagtcg tgcaactccc gcgccagccg gctgcgttcc tcacggcttg cggcctgccg
      421 gatctgttcc tcatattggg agcgcagccg gtccgtgtga gatcggttgg cgcggccgaa
      481 gaggctggtc agcggtgaga atggggttac tgagtcactc tccgcggtcc gccacaggta
      541 gaggaacacc aggaggatgc agttgagcac cgcggcaggc aactccgcgg gcagaggact
      601 ccagacggca tcctgctgaa gaagcacgac gacgaggatc acggcgtgtc ctgcggcgat
      661 ccaaagcaga ccacggcgtc tggatccggg atccggcacc gtcgcgagcg cggcggaaaa
      721 acaaccggca gcaaccagca tcgaccccag cacccggatc agcgacgccc tgatcgaaag
      781 ttccgcggca gggcctccgc cgagccacat gggaccccag gcgatcagca gaaagcctgc
      841 aaaccaggcg agggcagcgt acgcccaata gacgatgcgc gtgtcgaccg catcggcccg
      901 ttgtctcgtc cacatgccct cagtctacgg ctgcgccgcg agttccacat cacgcaatgg
      961 accgagtaga gcatcccccg tttgggggag gctccgaccg ggccggggct gcgaagttct
     1021 acagaattcc gccgccaatc gcaaggcgga agatgccaaa gacggtcgcg acgccgcagc
     1081 agacaacgcc gacggtagcg ccggtgcggt tgccatcgtt cgcggtggcg agcgcgacga
     1141 tgctcaccac aaggcccacc accgagaacg ggatgttgag ccaattcagc gacccgagac
     1201 aagggagaaa cgcgacgatc attccgagga tcgccaggat accccagacc atgctcacga
     1261 cctgcaacat ttgtttgctc tacctcctgg aggaaccggc cgcagcatgg taatcgcgaa
     1321 gacgacgcta ttgatcgcga aaaagacata cagcacaggc ccggccctgt ttgcggacca
     1381 tgcaccagat gcgtcgccgc gcgagaaccg gagaaagctg cgagtcaagc cacatgccgg
     1441 gcattcacgg ttgtatttcg ctttccattc acagacgggc gtccaaccgg cgatttgttt
     1501 ctctgcgaac agccacgggg cggccagcag tgcggcgccc gcgaggctga gtatcatcca
     1561 cacgattgtt agagacaacc gtacctgctg ccgggctggc attgggcgat tcatgaacgg
     1621 ctcgcccgcg cagtatatca ccccgtcacg cgcaacgggc tgatagactc aagagagtca
     1681 ggtatgttgt ccatcgtcat tccgattcac aacgaggagc cgtcgattct tccgctgtac
     1741 gaccggctca cggcggtgct cgagaagatc cataggccat acgagatcat tttcatcgat
     1801 gacgcgtcta gcgaccgcag tttcgacttg ctggccaacc ttgtggagac cgacgggcgg
     1861 ctgaaggtga tccggctgcg ccggaatttc ggccagaccg ccgcgctggc ggccggcttc
     1921 cacgaggcag agggggacgt gatcatctcg atggacggcg acctccagca cgacccggac
     1981 gacctcccgg cgctgctcgg taagatcgac gagggctacg acatcgcgag cggctggcgg
     2041 aaacagcggg tggacaacgc cgtaacccga aagattccgt cgcggatcgc gaactggctg
     2101 atggcgaagg cctcgggggt ggaccttcac gatttcggga cgacctacaa ggcctaccga
     2161 gcagaagtgc tgaaggacat caacctgtac ggcgagttgc atcgcttcat cccggcgctg
     2221 gcgagttttt atggcgcgcg tgtggccgaa gtgccgatcc gaaacacgcc gcgggcaacc
     2281 ggaggatcac attacgggct gagccgtacg tttcgagtgc tgttcgacat tatcaccatc
     2341 aagttcctgc tgaagtattt cacgcggccg atgcatttct tcggtaagct cgggctggcg
     2401 gggaccacag ttggcggatc gattctggcg tacctgttcg ttcacaaatt gatgggcgga
     2461 cacatcatga ccgagcacgg accgctgatg gtgctgggcg ggttgctgtt gctggcgggc
     2521 ttgatgatgt tcaccacggg cttgctcgga gaggtgctga tccggacgta cttcgagagc
     2581 cagggtcgcc gcatctatgc ggtccgggag atccgcaccc gccggaagcc gccggtcaag
     2641 gagaaggacc ggccggcgtg aagcgggcgg tagagcgccc ggaggcagac gactgactgc
     2701 gatcgtctgc cccaaccgag cttagaagtt ctgagcgcgg ttccagtggc cttcgaagag
     2761 acctctcatc gcctcgccga ggcctgggtg attgaacacg accgaggtcc aggtcggctt
     2821 ggtgattacc ggatcgagca gcgcgattaa accctgtgag ctatcgaaca acgcgagttt
     2881 caacggaaga gacgcagcct gacgaacctc gacgccggcc gcctggtatt cgccgagccg
     2941 ctgcctgtga tcttcactta gcgtgcccgc ctccagaagc agacggcagg cgacgccgcg
     3001 cccgcgggcc tcgcgtacga gttgttcgtc gagcgggtcc accgcgtaag gcggccttga
     3061 aaattcaaga tattcgcgct gcacttcggc gagcatgcgg cggtattggg tggcggtttg
     3121 cgtcggctca ctcacgattt gcaggaaatc gagggtgccg cgcccgccct tcacctcaca
     3181 gtaggcggtg ttgaggtcct cggtgaggcc cgccgccatg cgtccctgct cggtgagttc
     3241 gtgttcgagt tgatcgcgct tgcgggccag gtagcctggg atggcgagac ttggctccac
     3301 cgccgagaac agcttggttt tttcctgaac gacctgcgca aatcctttct cgacgaggct
     3361 gtcgagcact tcgtagatct tctgccgggg cacatgggcg cgagcggcca gttcgagcgc
     3421 cttgaaccgg ggatgaccca ccagcaccag ataggagcgc gcctcgtagg cgttgaggcc
     3481 gatctgctga agccgccgcc agaaccgttg gaagtcaatc gcaggaagtt cttcgttcat
     3541 tcaagcctca gaccattcac gatagcaaac agccgcactc ccgggtgggg gtgaagggga
     3601 ggcagtctga tacaatcacg ggtaagagca ctctgggggt gcaacaacat gacggcgcac
     3661 tgggacctcc cgcatacact gggcgcactc cggcgcagcg agttttccga cgagcggatc
     3721 gggcggcgca gcgtgaaaga cgaactgcgc gagaacctga tcgagcgatt ggggaccggg
     3781 gagacgctgt tccccggcgt ggtcggctac gacgacacgg tcattccgca gatcgtcaac
     3841 gcactgcttt cgcgccagaa cttcgtgctg ttgggactac gcgggcaggc caagacgcgg
     3901 attctccgca tgctaaccgg cctgttggac ccggcaacgc cctgtatcgc gggctgcgaa
     3961 atccatgaca gcccgtaccg tccgatttgc cgtcgctgcc ggaacctggt cgaagagatg
     4021 ggcgaggaga cgccgatcgc ctggatgaaa ccggagcagc gatacgtaga gaagctagcg
     4081 acgcccgacg taacgatcgc cgatatgctc ggggacatcg atcccatcaa ggcggcgcgg
     4141 cagggccatg acatttccga tgagctgacc gtccactacg ggctgttgcc gcgggcaaac
     4201 cggggcatct tcgcggtgaa cgaactgccc gacctcgcgg gcaagatcca ggtggggttg
     4261 ttcaacatca tgcaggaggg tgacgtgcag atcaaaggct accccgtccg gctaccgctc
     4321 gacctgctgc tcgtgttcac cgcgaatccg gaggactaca ccgcgcgcgg caagatcatc
     4381 accccgctta aggaccgtat cgggagtgaa atccggacgc attacccgct ttccgtggag
     4441 gacggcctgg cgatcacccg gcaggaagcc tgggttcagc gcccagcaac ggtgaaggta
     4501 gaggtgccgg cctacatcgc cgaggttgtc gagcagctcg cgtttatggc gcgtgaggac
     4561 aagcggatcg acaaacgatc cggcgtatcg caaaggctgc cgatctcggc cctggagaac
     4621 gtagtttcga acgcagagcg gcgcgcgttg cgatccggcg aggaccttgc agtgccgcgc
     4681 gtcctcgaca tttatgcggc gctgcccgcg atcaccggca agcttgaact cgagtacgag
     4741 ggggaactaa agggaggcga aacggtggcg cgcgagctgg tgcgaatgag cgtcgcaaag
     4801 gtctacacga cctacttcga tggcgcgaac gtgtcggcga tcgtccagtg gtttgagctg
     4861 ggtgggactc tgaagctgga cgagctcgcc tcttccggct cgcaagtgac tcaacttggg
     4921 tccattcaag gactgatgga cagaatcggg ctgttgggaa ttcgccccgg cgccgaagac
     4981 gccgagcgcg cttccgccgc cgagttcatt ctcgagggtc tgtacgccca ccggaggatc
     5041 agccgcacag aagagcgcgg cttcttcgcg gaagagaaga aacgcgaacc gattcctccg
     5101 gagccgggcc ggcgtcccgg caatcgcagg caatataact gaggggaggc gcccgttgag
     5161 atcaatccgg tattcgcgct acaccggcga ggatttcggc atcagcgctg aggatctgct
     5221 caacgcgctt tcggacttct ttcttcggag cggcttccag cacgatccct actcctttca
     5281 ggaatggaac gagagcagcc tcgagaacct caaggaggcg atccggcgcg cgctcgaaca
     5341 gggaatgctc agcaacgacg accgctttga gcagatgatg cgagcgctcg aaagcatgac
     5401 gcccgagcaa ctcgaccaac tgctcgaacg gctggcgcag aaacttgccg acgagggcta
     5461 tatcaacatc gagccacagc cggaagcgcc gcgggccggc acgctcggcg acgggcagaa
     5521 cgagcagcga atccgagtgg aagtgaccga caagacgctg gacttcctgg ggtacaagac
     5581 cctcaaagac ctgctcggct cgcttggaaa gtccagtttc ggcaggcacg acaccaggga
     5641 attggcgaca ggcgtcgagg cgtcgggggc ggccaagcgc tacgaattcg gcgacactct
     5701 gaacctggac gtgtgcgcga cgttgttctc ggcgatccgg cgtgaagggc cgggagtgcc
     5761 gctggcactc gagtatgaag acctgcacgt ccaccaggcc gagtaccaga gctcgtgcgc
     5821 gacggttctg atgctcgatt gcagccacag catgattctt tatggcgagg accggttcac
     5881 gccggcgaag aaggtggcgc tcgcgttggc gcacctgatc cgcacgcaat accccggcga
     5941 cagcttgcga tgcgtgctgt tccacgactc ggccgaagag ctgcgcctca gcgagttggc
     6001 gcgggtacag gtggggccgt actacacgaa cacgcgcgac gggctgattc tggcgcaacg
     6061 gctgctgcgt caagagaaga aggacatgaa gcagatcgtc atgatcaccg acgggaagcc
     6121 atcggcgctg acgctggacg acggacggat gtaccggaac gcattcggac tcgacccgct
     6181 ggtgatcagc cggacactcg aggaagtgag ccgctgcaag aaacaaggga ttctgatcaa
     6241 caccttcatg ttggcgagtg actacgggct agtagatttc gtgcagaagg tgacggcgtt
     6301 gtgccgtggc aaagcgtact tcaccactcc ttacacgctc ggcgagtacc tcctgctcga
     6361 ctatatgaac cggaagacac gaacgattca ttgagtcgaa catcccgctt gccggcgcct
     6421 gcgcgcggac ggacaatcgt ggtcgaagag gtggaatgaa agccacgctc attaccgcgg
     6481 tggctttggc agccctttcc ctcgggcaag cgcagcccgt gatcgatgtg ggcggggtag
     6541 tgaacaacgc gagttaccgg aaggctggca cgccgggata cgggatcgcg caagggtcac
     6601 tattcgcggt attcggagag aggctggggc cgacgaactt attcgttgcc ggggcctggc
     6661 cactcgggga gtcgctggca ggaacgtcgg tggaagtcac agtaggcgag gtcactctca
     6721 aggcgattcc ggtgtatacg tcggcacggc aagtcggcgc ggtgctccgc tcgcacacgc
     6781 cggcgggaga agggacgatc gtcgttcgtt acgacggcga gacaagcgct ccggcctcga
     6841 tacgaattgt tccccgcgct ttcggaattt tcacgcagaa ctcagccggc catggacctg
     6901 cgaatgcgca gcaggttcgc gcggacgcga ggcggccgat gaactcggcg tccgacccag
     6961 cgcgccctgg cgaattagtc tgcctctggg ggacggggct cggaccggtg agcggagacg
     7021 aggcgtcagg gccgcgaccg ggagacatgg ccgttgagtt taaggtgctc gtcggagacc
     7081 gcccagcaga ggtggtttac cgaggtcgct cggggtgctg cgccggcatc gatgaagtct
     7141 actttcacgt accggaaggc gttaaagact gcgcggcccc agttgttatc cgcagtggag
     7201 ggcacacaag caatctcgcg accctggcgg tcgccgatga acacggagtg tgcggaaatc
     7261 cggcgtttcg cgcgccgtga gggccaagct ccgctccctc acggccgcgt ttcctatcct
     7321 atgcgcgcgc gccggcggca gcaggcttgc ggaccacgcc caactgcgtt agcaagccta
     7381 gtgcgtccca gtttgaccac ccttccacaa tcttcccgcc ggaaatgcgg tccaagctga
     7441 ttccggtcac ggtcaccgac ttgcctgtcg cggcaacccc ttcgagttcg cctttgtgag
     7501 tcccagtcac cgtccagcgg gtagccacca tgtccccctg ctcgacgatc tgttcgactc
     7561 gaaagcgggt atccggaaag gccgttctgt acttccgcat tagctgcttt tgtccctgcg
     7621 gcccggttga aatgtcaggg ttcgctggat catgatgaac acaattggcg gcgatgagct
     7681 catcgagcgc cgcttcattc ccagaattcc aggcgtcgaa ataacgccgg acgtgagttt
     7741 cgttcatacc catgtacagt ccctccttac cacgattgta ttgtggaagc cgtggaggca
     7801 aaagtaacaa cttcagaggc ggttaaatag cctgaaaaca agtagttacg gccagggtca
     7861 gagcaactga agaaggccgt aaacaaccgg gatcgcaaac aacgtgctgt caacacgatc
     7921 cagccagccg ccgtgtccgg gcaggaggtt gctgctgtcc ttcacgccgg cgccgcgttt
     7981 caaggcggac tccgcgagat ctccgacctg tccggcgaca ttcgcggcga cggccaccag
     8041 aatcacctgg agtggcggca ccgacggcag aaaccagccg agatagaagt agccgaacag
     8101 cgccgacgcc acaaccgatg ccgcggatcc ttcccacgac tttgcgggac tcaatctcgg
     8161 agcaagcttg tgacggccaa acgcgctgcc gaagtaatac gccgccgagt cgccaatcca
     8221 gttcagggcc aaggtgaaga agagccagtg cgggctgatc tggcgcagcc cgatggtgaa
     8281 tcgccagcag ccgaagacat agacaatgcc gagcagaaac gcagcagcac ccggcaaggc
     8341 cttcgacaga tcgcgcgtcc gcaacgtgag cgtgagggcc agcagtacca gcagcgtgac
     8401 gagaagcaac tcgtgatcgc gcacgagcag gaccaggatc ccggcggcgt agccggccgg
     8461 tccaggggga tcgaagccgt gtcgcgcgac gatgctgccg tattcgtgga agcagagtag
     8521 cgcgacgatg gcgacaacgg ccaggaacag ccactgcggc gcaagaagag cgacgtaggc
     8581 gatggtgggg atcagcacca gcgcggtgac gatccgtctc atcgcgcagt cgcgcctacc
     8641 ccaacgagat ggctctcccg gccgggagaa ctgccggcgg gactcagccc accaaaccgg
     8701 cggtcccgct tctgatagtc gagaattgcg cggaggagct cggtacgagt gaagtccggc
     8761 caaagggtgt cggtcacgta caactcggcg tacgcgatct gccagagcaa aaagttgctg
     8821 atgcgcatct cgcccgacgt gcggatgagc aaatccggat cgggcagccc ggcggtatag
     8881 aggtgcgaag cgatggcgtt gtcgtccacg gtcagactat ccagccggcc tttgcggcgg
     8941 gcttcgtcga ggacggcgtt taccgcatcc acaacttcgt ttcttccccc gtaattgatg
     9001 gccagattca cctgaagacc gcggtttaaa gatgttgcct gtaccgccgc cgccaattcc
     9061 tcgcgtgcct gtgcaggaag cgcctggacg cggccgatgg ccgcaagacg aatgtcgttc
     9121 cgtcgcaggt tgggcagttc ccgccgcagg tagatgcgaa gcaggcgcca gagcgtgtca
     9181 acctcggccc ggggccgttt ccaattctcg accgagaagg cgtacagggt cagcgcgcga
     9241 ataccgagcc gtgcgcacgt ttccacagtg acacgcacag gttccacgcc cgccttgtgg
     9301 ccggccaccc gcggcaggct gcggcgcttc gcccagcggc cgttaccgtc catgatgacg
     9361 gcgatgtggg cgggaagacg gttcggatcg atcgcctcgg ccagcgacca gtccttctgg
     9421 cccggccgga ggacttcgaa cagagctttc atgaagtcaa cactctatac tacactgtca
     9481 ccgcagttgc ccgacggaaa gtggaagaca tgatacgacg tggactacca gcggttttcc
     9541 tcctcttctt cttggcctcc gcactgagtc cgcagaatcc gcccgatctg ggagctttcc
     9601 tgaaagcgga aatgcagcgc tcgaaaccgg actatgtcgt gtacgttccc ggcagcgtgg
     9661 atggatccac tttcgacacc ggaaacgagc acttcctggt tttcgacgcg ccggacggct
     9721 cgcttatggc gatctggacg caaagcacgt tcgagggtgc gggcgaccat cgaatcatgt
     9781 tcacgcgctc cacggatgaa ggcgaaacgt ggtccacgcc caagcgcctc gtcggcccgg
     9841 cgcgaaaagg tgagggacca atggcaagtt ggggattccc gctgacgtcg aagagcgggc
     9901 ggatctatgt cgtatacaac cagcagcagg aaacggcgga tgtccatccc cagatcactg
     9961 ggacgatgga ctgcatcttt tcggatgacc tggggacaac atggtcgcgg ccggagacga
    10021 taccgatgcc gcgtagctgt ttcgacagcc cggatgcttc gattgcctcg aactggatcg
    10081 tgtggcagaa gccgattcgt gacctgaagg accgctggtt caacggcttt acgcgctggg
    10141 tgagcaaggc ggtgcgcact ccgccacaca cgaactttag ctcgcacgag agcgtggtcg
    10201 agttcatgag gttcgagaac atcgacgcca acccgcggcc gccggagatc cgggtaacat
    10261 acactccaga cacccaggcg ctgcgcgtgc cgcactatac gaacccgcta ctgagcatcg
    10321 cgcaagagcc gagtctgctt cgacttccag acaagcgcct gttttgtgta atgaggagca
    10381 tgaccggcta tatctggtac agcgtctcgg gggacgacgg acagagatgg accaacccgc
    10441 gaccgctgct gcggcgagat cacggcctgc ctatccggca gccgctcagt tgtacgccga
    10501 tgtacgagat ctccgcgggc cggtacttcc tgctccacca cgataacgac gggcgctttc
    10561 agggaaccaa accggaagac acgagttgga accggaggcc ctgctatctt gtgctggggg
    10621 agttccgtaa gggggcggac cagccggtgt ggttcagcga gtcgaagttg ctgatggaca
    10681 ataaaggcgc acaactcggg ccactgaagc gaatcgacat cggcctctat accagcttca
    10741 cgaaccggtg cggcaacgcc gtcctgtggc acccggagcg gaagttcttc ctgctcggca
    10801 agcggatcac atctgatttc ctggcggacc tcaaggtacc ggaaaggtga ccctcgcatg
    10861 caaagaggaa gcaaagtacg atgagcgagc gtgccggcgg gacctacgag ttcggtccgt
    10921 ttcgttatga tgcgaaccag cgactgctgt tccgcgaggg cgagatcgtg ccgctcgcac
    10981 cgaagtcgct tgagatgctc caggcgctcc ttgagcggag ggggatggtg gtcgggaaag
    11041 ccgagctgat gaaactcgtc tggccggaca cgtttgtgga ggaggtagga ctcgcgcgaa
    11101 acatctcctt attacgcaag gtattggaag atgaaagcga agctaatact tacattgaaa
    11161 ccgtgccgag gcggggctac cgtttcattg ccgaggtcat tcggacggga gactgggagc
    11221 agtcttccgc tccccgacag acgagacgtc tctggtggcg ggtgatcctg gcagtcgtgc
    11281 tcgttggtct tgccggcatc atctactggc agttctatgc gccctcccgg tacctccccg
    11341 aaggattcgc gagcctcgct gtgtcgccct tcgagaccct gactccggat gccgaggccg
    11401 acgcgttttc ccagggattg accgaggtgc tggcagcgga actgtcgaag ctgaagggcg
    11461 tccatgtgat ctcgccgagc acggttcgaa ggtactggcg tttccggatc tccgcggcga
    11521 tgatggcgcg tctgctcggc gtccaggtga ttgtggaggg gacggttcag cgaagtgccg
    11581 accggctgat ggtgacagcg caactcaccg atgtccactc cgggaagctg atctgggcgg
    11641 agaacttcgc tgtcgcggcg gcggacccgg tcgccggccg aatgacgatc gcccgcgcgg
    11701 tggccgaaca ggtgggcgcc cgactggccg ttcggtgagc gtggagggca ggccaatggc
    11761 gaagcagcct acttcaatct gaagacgact atctccatat tccagttgtc aagcactgcg
    11821 aacgtggtac cgtccggccg cgatgctatg acttcggcgt cctggatgcc gaaaagccca
    11881 agttcttcgg aagtgtcgtc tggcgtaaac ggccttctac tcccccgcca gcggcggcat
    11941 ggtagcgcga aggaacgcga gcgcgagggc cgttagtccg aggaaggtac cgaagggaag
    12001 ctcatacgat gagaaatcct tcttgcttac cttgatatag accagcccta ccaccgagcc
    12061 gacgagcgag gcgaggatga tcgtctgaaa cgcaccgtgc acgcctagaa acgcaccgat
    12121 tgcgcccatc atcttgacgt cgccgaggcc gagcccggat ttatggcgaa ccttttcgta
    12181 aaggaaaccg accccccaga gcagcccggc cgctacccca gctcccagta tcgattcggc
    12241 cagccagagc caacggcccg tagtaagcgg gttcaagaag agaccggcga aaacgacgtc
    12301 catgggcacc accagcgcca gcagaagtcc ggcggccgtc ccgccaagcg tgaattcgtc
    12361 gggaaggatc cgctcctcga ggtcactgaa cgccagaccg atcatcaggg ccgaaaacag
    12421 gcagaccttg agggccggca gggtcggacc gagcgcaaga acggacaggg caaacgccag
    12481 agccgtcaga gcctcgacaa tcgggtatcg gaacggaatc cgtactccgc agtgccggca
    12541 ccgtccgccg agcagcgtgt agcttaccag cgggatattg tcgaaccagg caattttcac
    12601 attgcaggcc gggcagtacg accgcggcct cacgatcgaa agatccttcg gcagccggta
    12661 aatgcagacg ttcaggaagc tgccgatcaa caatcccgcg aagaaggcga ttacggcccc
    12721 gatcatctca gcacccactc gtagattttc accgtctcgc gcaccatttt ctccactgaa
    12781 aactcctcct gtaccacttg ccgcgcgcgc tcaccgagtc gccggcgcaa ttcctcatcc
    12841 tgcagcagcc tatgaatcgc ttccgctatc gccgcgggcc ggttttccac caacaacccg
    12901 gtttcgccgt cccggaccgc ttccggcaac ccaccgacac ggcttgccac caccggaatg
    12961 gcggccgcca tcgctagcag cgccgccgag cccaggccct cttcccgcgt gatgtagacg
    13021 aagagcgcgg cgcggcggag atcgccttcc aggttctccg agaagtgtac accgacaccg
    13081 gccattgccg ccgcgtcctc gacgagcgag cggcctttca tgggatcgcg tgtcgccggg
    13141 gcgacgacga ggcttccctg ccccgacggt tcacgcaccg gcacaccgtc gtggacgacc
    13201 gagactttgg cgtccgagat gccggcttcg atcagtctgt cccgtacgca gttggagatt
    13261 gcgatgtagt ggtctgcccg gcggtacttc cagcgggaga ccgcggaccg gcgtacgggg
    13321 aacgcgaccc gccgggccac aaccagcggc ttcaaaccga gcgccgccga gagagcgtgc
    13381 gagcgcccgt cgtgcacgtg caccgtgtca acatcgctcg ccaggcgccg gagcgagagg
    13441 atgcgggagg gttcgacgcg gatgccggac gcccgcgccc tcacggccag ctcggagtcc
    13501 tccggcgccg ccagtacagg ctcccattga gaggaaagca gcccctccga caggcgcagc
    13561 acctgccact ggcctccctt catttcgctg ccggtgtcga cgtggagcac ccgcatctca
    13621 gctcatgttt ctcgctttgg cgtacttgac gaagttgtag aaggccgcca tgtaggcgat
    13681 ggtgagccct tcgattccgt caagaaagcc ggcttgaaag acgtaggttc tcaagaacgt
    13741 ccacggcgga tcgagcagca ggtccgccag tgtgaccttc ttgccgcggg cgacgagatc
    13801 ctgggccgcg agcgtggtgt aacgttccat gcgcttgagg tgggaggaga tcgagtcgca
    13861 ggtgaagtgg agcagattgt agtcgaggtg accaacactc ccgctcacgt tgacactttc
    13921 atggacgaag tcccccgccc actgcgcgcg ccggcgatcg tacagacgca ccttgcggtc
    13981 ggggtaccag cccgagtgca gaatccagcg gccgagatac tgggccatgc gcggcatggt
    14041 ataggcgtcg aatttcggac cggatttctt gatctgccag atctcccctt cgagtgcttc
    14101 gctgagagct tcgtcaccat cgatggacag tatccagtcg tagctcgctt tttcggcggc
    14161 gaagttcttc tgcccggcat agcctcgcca cggcgattcc acgatccgcg caccgagttt
    14221 ggcggcgatc tccatcgtcc ggtcctgcga gccgctgtcc accaccacca cctcgtcgca
    14281 acagcgcaaa ctttcgatcg cccgggcgat gttgtcttct tcgttcaggg tgatgatggt
    14341 ggcggagatt ttcatggccc gatgtgtcag cccccactcg tttcaattgg cgccaccata
    14401 aaggagagca cgagcagcgc cagggctagg acgcccagcc tctttcgaat cggtccgagc
    14461 tcgctgtgat cgtacaccat cgggtgccgc atgccgaaga aaaacaggat ggcggcccag
    14521 agcagccacg gccagtaaac caggcccatc gggacgagcg aggcgacaaa gattctcgag
    14581 agcaaccggt ggcgttcgcc gaagaacgca tacagaatat ggccgccgtc cagttggccg
    14641 attgggagca gattgagcgc ggtagcgaag acgccgaccc acgcggcccg ggcgaccgga
    14701 tgcagcgaga tgtcctgcgc gggcacgccg gggaagatgg cccactgaaa aacgtggaag
    14761 atggcgggtg ttccgaaaat caggtctccc cgtgcgccaa gtccgggtac caccttggaa
    14821 aaggctagcc cgatcgcaag cgcgggaagt acgaagacaa agccggcaat cggcccggcg
    14881 attccgacgt caaacaagac cctcttggaa tagatcggcg cgcgaatacg gatgaacgct
    14941 ccgagggttc cgatgacggt cggcgagggc aggaagtagg gcaggctggc gtccaggcgg
    15001 taatgcacgc aagccgaata gtgtccgagt tcgtgcgcga gcaggatcaa gaggagcgtc
    15061 aacgagaacg gcagcccgtc gatccaggcg cgcgggtcgg taaagagacg gatgtaggat
    15121 tccaaatcct gcgagacgtc aatggccggc cggttgtagc gaaagttgtg ggctagtctg
    15181 gcgccgaccg cactcgtggt gaccagcgtg agcagaaaca gcagaacgtg caaccagtac
    15241 cggtgccggg ttcggctagc gccacgaaag aagtgggaga agcctctccc gtttacggcc
    15301 tccggcgaac tagggggtga cgggaactca tgcgctgaca cgaatttggc gcgttagagc
    15361 gactgaagct ccttgccggg cttgaaacgc acggcctttc cggggggaat cgcgacttcc
    15421 gcacccgtac gaggattgcg gccgattccg gttttccgag ggcggacatt gaagatcccg
    15481 aatccgcgca gttcgatccg ttctcccctg ccgagggctc gtttcatact ctcgaagacg
    15541 gtctctactg ccatctccgc cttcgtcttg gtgatgcctg tgcgattgac gacttcgttg
    15601 atgatatcca gtttgatcaa gggaacctcc cgcgtcgaga agcggtatcg agcaatatgc
    15661 cgcgtgtcgc gctgcacacg gcaaactaaa tgatatgatt agggttacat caatgtcaag
    15721 tgcgctgacg cacaagcatg catgaaactg ccgtccgcga ataaggatca attccgccgc
    15781 gcctgctcga aattcgccac cggaatcacg gtcgccagcg tgatcggcgt cgacggctcg
    15841 ccgcacgggc tgacggttaa ctcctttact tccgtatcgc ttacgccgcc cctggtgctg
    15901 ttctgcatcg acctcggatc gggtgtcctc cgccacttcc ggccggcgcg cttctttggt
    15961 atcaacgtct tggatcagca ccagcgttcg gtgtcatccc atttcgcccg gaagggccat
    16021 gaccggttta ccggaatcgc gtggctgccg gggaagactg gtgtgccgct gctacccggg
    16081 gcgatcgcga ctctggaatg caggacggtt cggagatttg ccgccggcga tcacctggtg
    16141 gttatcgggg aggctgtcac cgcggcggcg cacgagggaa agccgcttct ctacttcgca
    16201 agcggttacc acgaaattcc ctgaggagtt ccggcggaac gaaaagtggc ccgcggggac
    16261 gcgggccacg ggtcattcac cggaaatcac agggctgctt ttgccgcggc gagcgcggtg
    16321 tcgtagttcg gatggtcgcc cacctccttc acgtattcga cgtgacggat tacgttgttc
    16381 ttgtccacca caaagatcgc gcggctctca atgcgcaact ccttgatgag cgtgccgtag
    16441 gccgagccaa aggaaccggt cttgtggtcg gaaaccatct tcacccgatc cacgccaaag
    16501 gcgccgcacc accgcttctg cgcgaacgga aggtccatgc tgatcgtgta cacttcgacg
    16561 ttcggcagct tcgccagctc gtcgttgaag cgtttggtct gcgcgtcgca gaccggggtg
    16621 tcaagggaag gcacaacgct gaaaatccgg accgagttac cggtcttcgc cagattgact
    16681 gcgctcaggg tggtgtccac cacctcgaag tccggcgcct tgtccccggg cttcagcgcg
    16741 ggaccgatca aagtcaaagg atttcccttc agagttgtag ctcctgctct ctccatctgt
    16801 cctcctgaat gtgttgttgg ttgaagtgct ggttgcgtat tattttagca actccgagag
    16861 ctgctccggc tcggtcaccg gtaatttgca agtgaagtcc tcgcacacat aagcggctgc
    16921 tctcccgccg acccgcctca taccggcgat ggccgggacg ttgcgcgaca gcgcctcccg
    16981 gacgtcttca gaatgtacga gcaacacgac cttgttcgga tcgaagcgcc ggcggacggc
    17041 ctgcagcaac gccagcgcgt ccacagcgtc gcgggcgccg acgacgacga tctgcctctg
    17101 cttcgcgagg taatgatcga gcgtcacaag catttgcggg atcccggcgg gcatcgcctg
    17161 tagccgcgag gcgaagacaa gcagcgtctt ttcggccgcg acgcgaaaat cgtccctccc
    17221 cgtcatctgc gcgaggcgga gcagattcag cgcggcgatc gaattaccgg agggttcggc
    17281 gccgtcgtag tcgtccttca ttcggagcag caggctggca tcgttgtcgg ccgtactgaa
    17341 gaagccgccg tgttcggggt cctcgaacca ctcaccgatc tcttcggcga gccgctcggc
    17401 cgtttccaga tagcggagct cgaaacaggt ctcgtacagg tcgacaagag cctgcgtgaa
    17461 aaatgcgtag tcgtccagga agccggagat ggccgcctcg tcatcccgat agcgccgcat
    17521 gaggacgccg gtgtggccgt cgaacagcct tcccaggatg aagtccgccg cccggcgcgc
    17581 cgcagccagg tagcgaggtt cgtcgagcac ttgcgctccc aatgcgaacg cagagatcat
    17641 cagcccgttc catgctgtca gaatcttgtc gtccaggtga gggcgaaccc gcttcgatcg
    17701 cgcctcgagc agcacgttcc tcgacgcggc aagcgacgcc aggacttcgt ccacgcccgc
    17761 cccgaagcgc gcggcgacct cggtggcggt tcgagcctcg tacaggatgt tcttgccggt
    17821 gaattcgtcc tgcggatctt ccgcgacgtt acccgtttcc tggacgccat aggcgtagca
    17881 gaaccattcc gccgccggac ttctcagccg cgctgcgatc tcgtcctggg accaaacgta
    17941 gaaagcgccc tcgcctttca cgtccggttg agcggggtcg attacgctgt ccgcgtcctc
    18001 cgccgaatag aagccgcctt cgggatgagt catgtcccgc aggacatact caagtacgtt
    18061 ccgcgcgaca gccgcgcatg acgcctggcc ggtaatctga aatgcctcga gataggaagt
    18121 cgccagttgc gcctggtcgt acagcatctt ctcgaagtga ggcacgaacc aacggtcatc
    18181 gaccgagtaa cggtggaacc ctccgccgag ctgatcgttc attcctccac gcgccatcgc
    18241 ctgaagcgtc ttgagcacca tctcgagggc ctcgtgattg ccggaacggg caaagtagcg
    18301 cagcaggaag ttgtggaccg acggccgagg gaacttcggg gcgccgccga agccgccaag
    18361 gttgggatcg aagattcggc ggaaggcgtg gtatccgctt tcgagcgggc cgtcgttcaa
    18421 aaatgccgcc gacggtgtca cctcggactg cttccggagt tcctcgacaa tcgcagcgcc
    18481 ggactcgacc agcctggctc gatcggcccg ccaagcctcc acaatgcgct ccagaacaaa
    18541 cgcgaagccc gggcgcccgt agcgggtgtc gggaggaaag tacgtgccgc cgaagaaggg
    18601 cttcaggtcc ggtgtgagcc agacggacat gggccagccg ccgctgcccg tcgtcacctg
    18661 aacgaagagc atgtatatgc ggtcgacgtc cggccgttcc tcgcggtcca ctttcaccgg
    18721 gacaaaatcc cggttcagga tttccgcgat ccgatcgtct tcgaacgatt cccgttccat
    18781 gacgtggcac cagtgacatg tggagtagcc gactgacagg aagatgggct tatcctcccg
    18841 gcgcgccacg tcgaacgcct cttcgcccca ggcgtgccag tccaccggat ggtgggcgtg
    18901 ttgaagcaga tagggactct tctcctgtgc gagtcggttt gtgtgcattc ccccgaagct
    18961 acctgacgcc ggccgtccag gcggcccgat ccggcaagta gcgctcctct ctgagtttgt
    19021 cgagcacggc ggcgagcttg tcgcgagcgg agtcgggcat cggtcccacc ggacgccggc
    19081 acctccccac ag
//
LOCUS       DATIFQ010000065         5733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_595623, whole genome shotgun sequence.
ACCESSION   DATIFQ010000065 DATIFQ010000000
VERSION     DATIFQ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595623"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(590..1744)
                     /locus_tag="VLE22_02875"
     CDS             complement(590..1744)
                     /locus_tag="VLE22_02875"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HSB13373.1"
                     /translation="MTIGVPKETYPGERRVALVPTALQPLAKAGHQVIIESGAGTSAG
                     FPDSEYTEKGARVVSSRADVFTAADVIAQVRTYGSNPEAGKEDLSLYRSGQVVIGFFD
                     PLSFPAHLREVAPTGVTAYSMELMPRITRAQSMDALSSMATVAGYKAVLLAAERLPKL
                     FPMLTTAAGSIVPAKVFVIGAGVAGLQAIATARRLGGVVSAYDVRPAVREQVQSVGAK
                     FIELPLETAGAEDKGGYAQAQDESFYRRQRELMGRVVAESDVVISTAAVPGKKAPVLI
                     TRDMVAGMAPGSVIVDLAAERGGNCESTRMDEVVVDNGVTVLGPGNLPATVPYHSSQL
                     YAKNITNFFVHLAKNVLSQPDNEDEIGRDTLLTRGGAVVHPRILELLGNQ"
     gene            complement(1791..2705)
                     /locus_tag="VLE22_02880"
     CDS             complement(1791..2705)
                     /locus_tag="VLE22_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13374.1"
                     /translation="MVTTTSRRRLFQSAAGVALAGSRLSAAVSPALPTVRLGSHDITR
                     LIIGSNPFNGYSYGIYSLDHHMREWSTEENIAKTLRMAEQSGINTHQFSYNPKGMAGL
                     KRHQAEGGKLNWILLGGGEMKDNLALIPQTAKLGPLGIVHHGGVTDQRFRAGEIDKVH
                     TFLKTVRQSGVLVGLSTHDPKIIEYVEERNWDIDFYMACFYQFSRTKEEIKQMLGELP
                     LGKVFLEGDPARVCKVIRATRKTCLGFKILAAGRSAESPKELEAAFRFAFDNIKPKDA
                     VIVGMYPRFKDEVRENADIVRRVAGLPT"
     gene            2849..3406
                     /locus_tag="VLE22_02885"
     CDS             2849..3406
                     /locus_tag="VLE22_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB13375.1"
                     /translation="MTEPATGIVDRDLPDEGTVEVFAALVREHQAMVYSLAHHFLRDP
                     GAAEELAQEVFLQLYEHLDDIESPAHAAHWLRRVTSHRCIDRARRRSHRPEAREVQLT
                     EVVEPRSPAESSDPLLAEKLRKLVASLPEKPRMVVILHFQEDLDASEIASVLNMPVST
                     VRSHLQRALVMLREKAARTLGDLKS"
     gene            3403..3750
                     /locus_tag="VLE22_02890"
     CDS             3403..3750
                     /locus_tag="VLE22_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13376.1"
                     /translation="MKRMEDELRQALQRHSPPEGFADRLLAKAARRPARAEGRRNRRA
                     FGWYAWRWASVAAVVLAMIAGVRYQRIEAARGMAAKEQLVLALRITAAELQFAQEKVL
                     EIGREGHPSAVAH"
     gene            3764..4312
                     /locus_tag="VLE22_02895"
     CDS             3764..4312
                     /locus_tag="VLE22_02895"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HSB13377.1"
                     /translation="MKIVVVCIVLALSTALAQEIEFPPSFQKLAEKAVESVDVTLDGA
                     MLKAASKFLSEKKTEEGEAKKLVSGLKAIRVRSFKFAQEAEYSEADVAPIRSQLSSSS
                     GWSRVVSAKSGRENAGVYLRKEGDAVVGLLVLAMEPKELTVVNIVGPIDLDQLSNLGG
                     SLGIPRVSIKSKEKDQKEKDDE"
     gene            4390..5499
                     /locus_tag="VLE22_02900"
     CDS             4390..5499
                     /locus_tag="VLE22_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB13378.1"
                     /translation="MTHQRREFLQRAFLTAAATATYSSCAKAVPGKLGIPGTFRGRVV
                     AVEHPGSISSAGYQREPVRQMVHKGMMELTGAPSPQEAWRHFFQPGDVVGIKVNPVGQ
                     PHVISSAEVFQEIVEGLKMAGVKTRDIVAYDRYKKMFVTGGFEKWLPEGVRWTWATEN
                     FHELQLDMDGYDETHYMETQVVLPKQNPSDPHHRRSYVAKFLTKDVNKMVNLGVLKHH
                     QSAGITLALKNMSHGLVNNVSRSHSTPTLNTCGTFIPAVVDIPIIRERVVLHILDGVL
                     GAYHGGPGGNVKNYMWNHQTMYFATDPVAMDRIGWKVIDAKRTEMGMKPVGEAPPDKD
                     STFYRMQPEHVEIAGVLGLGEYDDKKIDLRKFKLA"
BASE COUNT         1153 a         1711 c         1798 g         1071 t
ORIGIN      
        1 tcgtagggtg gagcgccagg gtcatgagtc caaactgtaa tattccgcca gccgttggat
       61 ctcctcgtta acacgcgaat gggaggactg gtggtgagaa tacttgccac tcttctccaa
      121 gaggccgctt gaggcgtcaa aacaagggtt gggcagcctc cgctgccaca ccacgagctg
      181 ccgagcaaga aaacgattgc ctcatctttc ccatcagcgt ttagatcaac aaaggctctc
      241 gcataacgcg taacatcctc acctggaaac tgcttttgcc tgacgtactc ccggagaaac
      301 gttcttagag actcttcccg tggcaccgat gatatcgcgg gccgcggtgc ggactgtgag
      361 aaacatgtaa ccgccagagc cgtcaagcca catattcgaa atggcgaacg cgccagccaa
      421 gacgcggaca aggttttccg catacgtcca cctcaacgga agcagtccat cagtttcatg
      481 acggtgcttc cgtagttgcc atgaagccga ctacctacat cgatcgtgcc caggtctgtg
      541 gcagctcacg tggggcggac gatcgcattt tgtcgtccgc ctgcgactct cactggttgc
      601 cgagcagctc caggatgcgg gggtggacca ccgctccccc gcgggtcaag agcgtatcgc
      661 gtccgatctc gtcctcgtta tccggctgcg atagaacgtt cttggcgagg tgcacgaaga
      721 agtttgtgat gttcttcgcg tacagctggc tggagtgata gggcaccgtc gccggcagat
      781 ttccaggccc gaggacggtg actccgttgt cgacgacgac ctcatccatg cgggtcgact
      841 cgcagttccc gccgcgctcc gccgccaggt caacgatcac ggagcctggc gccattccgg
      901 cgaccatatc ccgagtgatc agcaccgggg ccttcttgcc gggcacggca gcggtggaga
      961 tcaccacatc gctctcggcc accacgcggc ccatcagttc cctttgccgg cggtagaacg
     1021 attcgtcctg tgcctgggcg tagccgccct tgtcttccgc ccccgctgtt tcgagcggca
     1081 gctcgatgaa cttggcgccc acgctctgca cttgctcacg caccgcgggc cggacgtcgt
     1141 acgcgctgac cactccgccg agacgccttg cggttgcaat cgcctgaaga ccggctacgc
     1201 cggcgccgat tacaaaaacc ttggcgggca cgatactccc cgcagccgtt gtcagcatag
     1261 ggaagagctt cgggagccgc tctgcggcca gcagaacggc cttgtatcct gccaccgtgg
     1321 ccatcgacga cagggcgtcc atgctctgag cgcgcgtaat acgcggcatc agttccatgg
     1381 aatacgcagt aacgccggtg ggcgccacct cccggagatg ggcaggaaac gagagcggat
     1441 cgaagaatcc gatcacaacc tggccggacc gatacaggga gagatcctcc ttgccggcct
     1501 ccggattgct accgtaagta cgtacctggg cgatcacatc ggcggccgtg aacacatccg
     1561 cccgcgagga aaccacgcgt gctcccttct ccgtgtattc ggaatccgga aagccggcgg
     1621 atgtgccggc gccggactcg atgatcacct ggtggcccgc tttcgcgagg ggctggagcg
     1681 cggtcgggac gagcgctacc ctgcgctctc ctgggtatgt ttccttagga acgccgatgg
     1741 tcataagttg tacagcttac caacgtgcgg gcctcccgtg tgggaccgcg ctatgtgggc
     1801 agaccggcga cgcgccggac gatgtccgcg ttctcgcgga cctcatcctt gaaccgcggg
     1861 tacatgccga cgatcaccgc gtctttcggc ttaatgttat cgaaggcgaa gcggaacgcc
     1921 gcctccaact ctttcgggct ctctgccgag cggccagcgg cgaggatctt gaatccaagg
     1981 caggttttcc tggttgcgcg aatcacctta cacacccggg cgggatcgcc ctcgaggaag
     2041 acctttccga gggggagttc gccgagcatc tgcttgatct cctccttggt ccggctgaac
     2101 tggtagaagc aggccatgta aaagtcgatg tcccagttcc gttcctccac gtactcgatg
     2161 atcttcgggt cgtgggtgga gaggccgacc agcacaccgc tctgccgcac ggtcttcagg
     2221 aacgtgtgga ccttgtcgat ctcgccggcg cgaaagcgct gatcggtaac gccgccgtgg
     2281 tggacgatgc cgagcggacc caatttggcg gtctgcggga tcagcgcgag gttgtctttc
     2341 atctcgccgc cgccgagcag aatccagttg agcttgccgc cctccgcctg atgacgcttc
     2401 agccccgcca ttcctttcgg gttgtaactg aactggtgag tattgatgcc gctctgctcc
     2461 gccatgcgca gtgtcttcgc gatgttctct tccgtcgacc attcccgcat atggtggtcg
     2521 agcgagtaga tgccatagga gtacccgttg aacggattgg agccgataat caaccgggtg
     2581 atgtcgtgac tgcccaacct gaccgtcggc aaagcaggcg acaccgcggc ggacaagcgg
     2641 ctgccggcga gagcaactcc ggcggcggac tggaacagcc tacgtctgga tgtcgtggta
     2701 accatgggac ctcccaccga gtttcagacc tcgaattctc actttatacc agaagtctgg
     2761 aatttcgtag ccgggagccg tcgaaaaagt tgcgttgcgg aacccgccct agcgcgtatt
     2821 ggatactgag agggaaagag acgtcggcgt gacggagcca gcgactggaa tcgtcgatcg
     2881 ggatctgccg gacgaaggca ccgtcgaagt gtttgccgcg ctggttcgag agcaccaggc
     2941 gatggtgtac agtctggcgc accacttcct gcgcgaccca ggagcggccg aagaactcgc
     3001 gcaggaggta tttctccaac tgtatgagca tctggacgat atcgagtcgc cggcccatgc
     3061 cgctcactgg ctccgaaggg tcaccagtca ccgttgtatt gaccgcgccc ggcgacggtc
     3121 gcatcggccc gaagcgcgtg aagtgcaact caccgaggtt gtggagccac gatcgccggc
     3181 cgaaagcagt gatccgctgc tggccgagaa actccgaaaa ctggtcgctt cactgccgga
     3241 gaaaccccgg atggtggtga tcctccactt ccaggaggat ttggacgcct cggagatcgc
     3301 ctctgtgttg aacatgccgg tctcgacggt ccgcagccac ctccagcggg cgctggtcat
     3361 gctccgcgag aaagcggcgc gcactcttgg agatttgaag tcatgaaacg catggaagac
     3421 gaattgcggc aagcattgca gaggcactcg cctccggagg gattcgcgga tcggctgctg
     3481 gccaaggcgg cgcggcggcc tgcccgcgcg gaaggccgac ggaatcgccg ggctttcggc
     3541 tggtacgcct ggcgatgggc atccgttgcg gcggtggtgc tggcaatgat cgctggagtc
     3601 cgttaccaaa ggatcgaggc ggcgcggggt atggctgcaa aagaacaact ggttctggct
     3661 ctgcggatca ctgccgccga gctccagttc gcgcaggaga aggttctcga gattggccgc
     3721 gaagggcatc cgtccgctgt ggcccattga tcagggaggt ctcatgaaaa tcgtagttgt
     3781 ttgcattgtg ctggcactct cgaccgcgct ggcccaggag atcgagttcc cgccgagctt
     3841 tcaaaagctg gcggaaaagg cagttgagtc cgtcgacgtg accctggacg gcgccatgct
     3901 aaaggcggcg agcaagttcc tgtccgagaa aaagacggaa gagggcgagg ctaagaagct
     3961 ggtgagcggc ctgaaggcca tccgtgtcag gagcttcaag ttcgcccagg aggctgagta
     4021 tagtgaggct gatgtcgctc cgatccggtc acaattgtcc tcttcttccg gttggtccag
     4081 agtggtgagc gcaaagagcg ggcgcgaaaa cgccggggtc tacctaagaa aagagggtga
     4141 cgcggtagtg ggtctgctcg tgctggcgat ggagccgaag gagttgacag tcgtcaacat
     4201 cgtcgggccg atcgacctgg accagctcag caatctcggc ggaagcctcg ggatcccgag
     4261 ggtgagcatc aagtccaaag aaaaggacca gaaggaaaaa gacgacgaat aggaacgcac
     4321 cgcgtatcag acttccatag cccgtcaccg gactgtacaa taggaccaat actcagagga
     4381 ggtcgtctgg tgacacatca acgacgggag tttcttcagc gtgcgttcct gaccgcggcg
     4441 gcgacggcga cgtattcgag ttgcgccaag gcggtcccgg gcaagcttgg catcccaggc
     4501 acgttccgcg ggcgggtggt ggccgtcgaa catccaggca gcatcagttc cgcagggtat
     4561 caacgcgagc cggtgcggca gatggtgcac aagggcatga tggaactgac cggcgctccc
     4621 tcaccgcagg aagcatggcg acacttcttc cagccgggcg atgttgtcgg catcaaggtc
     4681 aaccccgtcg gtcagccgca cgtcatctcc tcggcggagg tcttccagga gattgtcgag
     4741 gggctgaaga tggcgggcgt gaagacacgc gacatcgtcg cctacgaccg ctacaagaag
     4801 atgtttgtca ccggcggttt cgagaagtgg ctacccgagg gcgtgcggtg gacgtgggcc
     4861 accgagaact tccacgaact gcagctcgac atggacgggt acgacgagac ccactacatg
     4921 gagacgcagg tggttctgcc gaaacagaac ccgagcgatc cgcatcaccg ccgttcctac
     4981 gtggcgaagt tcctcaccaa agacgtcaac aagatggtca acctgggcgt cttgaagcat
     5041 catcagtcgg ccggcattac attggcgctg aagaacatgt cgcacgggct ggtgaacaac
     5101 gtaagccgaa gccattcgac acccaccctg aacacctgcg ggacgtttat tcccgcggtc
     5161 gtcgacatcc cgatcatccg ggagcgcgtt gtgctgcaca tcctggacgg cgtcctcggg
     5221 gcgtaccacg gcgggccagg cggtaacgtg aaaaactaca tgtggaacca ccagaccatg
     5281 tacttcgcga ccgacccggt ggcgatggac aggatcgggt ggaaggtcat tgacgcgaaa
     5341 cgcacggaga tgggcatgaa gccggtcggg gaggcgcctc ccgacaagga cagcacgttc
     5401 taccgcatgc agcccgagca cgtggagatc gccggcgtgc ttggtctcgg tgaatacgac
     5461 gacaagaaga tcgatctgcg gaagttcaag ctagcctgag cccggggcag tccgccccgc
     5521 cagccggata taatccgttc ctggacggaa cggaatgatg agaaacgctc tacttttgtt
     5581 gatcgcaggt tttgtgtgcc tgccctccgg ggccgagacg cgcttcgagc gccgggctcg
     5641 ggagaaacag gagctcgccc gccagatcgt ctggcccaat cacgagcccc tggttttcta
     5701 tctgcgccgc ggtggccggg ccgagaactg gcc
//
LOCUS       DATIFQ010000066         4399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1171280, whole genome shotgun sequence.
ACCESSION   DATIFQ010000066 DATIFQ010000000
VERSION     DATIFQ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171280"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VLE22_02905"
     CDS             complement(<1..461)
                     /locus_tag="VLE22_02905"
                     /inference="COORDINATES: protein motif:HMM:NF018123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HSB13379.1"
                     /translation="MEGNFGIASLLILISGVLNASFPLPMKFSREWRFENNWLAFTTL
                     ALLAVPAGLAMMIVPEFRQIYTAASPGEFLPGIVFGFLWGTAQVTFGLSIEMVGMAMA
                     FAIVVGMSAVIGSLTTLLVLNPEEALGSRGLILMASAVILAAGLMLYAKAG"
     gene            complement(532..1383)
                     /locus_tag="VLE22_02910"
     CDS             complement(532..1383)
                     /locus_tag="VLE22_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB13380.1"
                     /translation="MKMTTLILACFVAATPVFAQSSGVTILQQIRSHRDGVALWWLGN
                     DGWLIKSGGVLLATDLDLESSERLQPPAISTAELADVLDVAFVTHHHGDHFNEPTLKI
                     LGREARCTFVLPKTCLKGAGEIGLPKERIIVPQPGRPFEVKGVRVEPTHAIHGNQEFT
                     VLTREPDFVESIAANCGYVLHLGGKKFFQPGDSVLTEEHLALKDIYALFVSPTVHNMY
                     VDRSMILINKLEPAYIFPQHFETYQSSEDNAFWTRGYPDELKLRLSGELQKRYHKLKQ
                     GEMFVVR"
     gene            1471..3684
                     /gene="hepB"
                     /locus_tag="VLE22_02915"
     CDS             1471..3684
                     /gene="hepB"
                     /locus_tag="VLE22_02915"
                     /EC_number="4.2.2.7"
                     /EC_number="4.2.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF045571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015021 - heparin-sulfate lyase activity
                     [Evidence IEA];
                     GO_function: GO:0047488 - heparin lyase activity [Evidence
                     IEA];
                     GO_process: GO:0030211 - heparin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparin/heparin-sulfate lyase HepB"
                     /protein_id="HSB13381.1"
                     /translation="MHVIALLLALACAPPMAAQPSKIVDAKWMDAGGVSVPAPPAEHP
                     RVFLRSRDIDDLRRRTTHPALKPVWDEMQKLAESNVQIRIEVDAARYLLTRDAELGRR
                     TVALALATLQKAEYDPKAQDVTRPIGRMLVTGSVAYDWCYPLLTAEQKQAFLDQILRF
                     ARQLECGYPPRPMGWVTGHGSEWMVMRDMLAAGLAIYDEDPEMYRHAAVTFFIGLLPA
                     RNFWYPGHAFHQGTSYSETRFSSDLYPLWIFDRLGAGNVYHPAQQFVPYQWIYLRRPD
                     GQLLRAGDGQSREPKLRSLLSASYYGDGYVLADYLRNPGIDPMNKLFQFLWSDPDLKP
                     LPASDLPLTRYMGFPYGWMVARTGWGEDSVIAEMKVNAYYFGNHQHLDAGAFQIYHKG
                     PLAVDSGNYQGTSGAYGSPHHSNYYRRTIAHNSLLIYDPSEKFASSASRDFRNDGGQR
                     LPNNWREPATLDVLLSRGYKTGEVGGYWFGPDPQRPAFSYLKGDITEAYSAKVKEVKR
                     SFVFLNLAGEQTPAALIVFDRVVSADPSFKKYWLLHSMEEPEVREERTIVSLSERGWS
                     GRLINHTLLPEPVDAEFVKVGGPGKEFWVFGENFPNDISRGDPNTYELGAWRVELSPK
                     RPSSEDLFLNVIQITDRERNSVLPVAKVEAGPMVGVRIADRLVLFARTGRPATRPVAF
                     TATGEDTLKYLVTDLSEGTWQVWRDGRILHPAIHVTGSAGSLYFEGPPGEYSLRR"
     gene            3717..>4399
                     /locus_tag="VLE22_02920"
     CDS             3717..>4399
                     /locus_tag="VLE22_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB13382.1"
                     /translation="MNRRQFLHTSLGTVAGLVLPRSALPGNPPDLKITGIGFLHLQFP
                     GRTPRQRNSIIESGGGPPGMSQLEIYTDQGITGRSLSGRNSIIENQLFARIKGENPFY
                     IERIWDRMYRHNRKPVAKGEYIRAMGAVDIAIWDIIGKALNLPVYKVLGAYNEKLRVY
                     AAGGYYQEGKGLKELAAEMEGYVGEGFRAVKMKVGWGPMRQDAERVRAVRDAVGPDID
                     VLLDANNAW"
BASE COUNT          839 a         1438 c         1305 g          817 t
ORIGIN      
        1 ttgcccgcct tcgcatagag catcaacccc gccgccagga tcaccgcact cgccatcagg
       61 atcagtcccc gagaccccaa cgcttcctcc ggattgagca ccaatagggt cgtgaggctc
      121 ccgatcaccg cgctcatacc gaccacaatg gcgaacgcca tcgccatgcc gaccatctcg
      181 atgctcagtc cgaacgtcac ctgcgcggta ccccaaagaa agccgaatac gatgcccggc
      241 agaaactcac cgggcgaagc agcggtgtaa atctgccgaa attccggcac gatcatcatt
      301 gccagtcccg ctggaaccgc cagcagagcc agggtggtga acgccagcca gttgttttca
      361 aagcgccact cccgcgagaa tttcatcggg agtggaaagg aggcgttcag gacgccgctg
      421 atcagaatca gcagggacgc gatgccaaaa ttgccttcca tgatatgagg acccgattgt
      481 aattcaattc tcgtcaccgg atcaggcagg catggtcgcc gcaatggccg ctcatcgaac
      541 cacgaacatt tcgccctgtt tcagcttgtg gtatcgcttc tggagctccc cggagagccg
      601 cagtttcaac tcgtcagggt aacctctcgt ccaaaacgcg ttgtcttcgc tggattgata
      661 ggtctcgaag tgctgcggaa agatgtacgc cggctccagc ttgttgatga gaatcatcga
      721 ccggtcgacg tacatgttgt ggaccgtcgg tgagacgaag agcgcataga tgtccttcag
      781 cgctaggtgc tcctcggtca gcacggagtc ccccggctgg aagaacttct tcccgccaag
      841 gtggaggacg tacccgcagt tggcggcgat cgactcgacg aaatccggct cgcgcgtgag
      901 caccgtgaac tcctggttgc cgtggatggc gtgcgtcggt tccacacgga ctcccttgac
      961 ctcgaacggc cgccccggtt gaggcacgat gattcgttct ttcggcagac cgatctcccc
     1021 ggcgcccttc aggcatgtct tcggcagcac aaacgtgcac cgggcttcgc gcccgaggat
     1081 tttgagcgtg ggttcgttga agtggtcccc gtgatgatgc gtgacgaagg ccacatcgag
     1141 cacatccgcg agctccgccg tcgagatcgc cgggggttgg aggcgctcgc tcgattcgag
     1201 atcgagatcc gtcgccagga gcacgccgcc ggatttgatc agccagccgt cattgcccag
     1261 ccaccacaac gccacgccgt cgcggtgtga acggatttgc tggaggattg tcacgcccga
     1321 agactgggcg aagaccggcg tcgccgccac gaagcacgca aggatcaaag ttgtcatctt
     1381 catgcggttc tcgcttgtgc atggtagcac ttccatcgcg gcgtcctccg gttccggtgt
     1441 gatactctgc gcctctggtc aggagatttc atgcacgtca tcgctctttt gttggccctc
     1501 gcctgcgcgc cgccgatggc ggcgcaacca agtaagattg tcgacgcgaa gtggatggac
     1561 gccggagggg tctccgtccc ggccccgccc gccgagcacc cccgcgtatt cctgcgatcc
     1621 cgcgacatcg acgatctccg ccgccgcacc acccatcctg ccctcaagcc ggtttgggac
     1681 gagatgcaaa agctcgctga gagcaacgtg cagatccgca tcgaagtcga cgccgcgcgg
     1741 tacctcctca cgcgcgacgc ggaactcggc cggcgcacgg tcgcactcgc gctcgcgacc
     1801 cttcagaaag cggagtacga tcctaaagct caggacgtca cccgcccgat cggacgcatg
     1861 ttggtcaccg gctcggtcgc ctacgactgg tgctatccgc tgcttacggc cgagcagaaa
     1921 caggcttttc tggatcaaat ccttcgcttc gcccggcagc tcgagtgcgg ttacccgccc
     1981 cggcccatgg gctgggtgac cgggcacggt tccgagtgga tggtgatgcg cgacatgctc
     2041 gccgccgggc tggccatcta cgacgaggat cccgagatgt accgccacgc cgcggtcacc
     2101 ttcttcatcg gcctactacc cgcgcgcaac ttttggtatc cgggccacgc cttccatcaa
     2161 ggcacgtcgt actcggagac tcgtttcagc tcggacctct atcctctttg gattttcgac
     2221 cggctcggcg cgggcaacgt ctaccacccc gcgcagcagt tcgtccccta tcagtggatc
     2281 taccttcgcc gtcctgacgg acagttgctt cgcgcgggcg acggccagag ccgcgaacct
     2341 aagcttcgct ccctcctgag cgccagctat tacggcgacg gttacgtgct cgctgattac
     2401 ttgcggaatc ccggcatcga tccgatgaac aagctctttc agttcctctg gagcgacccg
     2461 gacctgaagc cgctgccggc gtccgacctt cccctcacgc gctatatggg attcccgtac
     2521 ggctggatgg tggcccgaac aggctggggt gaagacagcg tcatcgccga gatgaaggtg
     2581 aacgcctact acttcggcaa ccatcagcac ctcgacgcgg gagcgtttca gatctaccac
     2641 aagggtcctc tggcggtgga ctccggtaac taccagggca catcgggcgc ttacggcagt
     2701 ccgcaccaca gcaactacta ccggcgcacg atcgctcaca acagcctgct gatctacgac
     2761 ccgtccgaga agttcgcttc gtccgcctct cgcgacttcc gcaacgacgg cggccagcgt
     2821 ctcccgaaca actggaggga gccggccacg ctcgacgtgc tgctttcgcg cggatacaag
     2881 acgggcgaag tcggaggcta ctggttcggc cccgatcctc agcggccggc tttttcgtat
     2941 ctgaagggcg atattaccga agcctacagc gccaaggtga aagaagtgaa gcggtccttc
     3001 gtattcctga acctcgcagg agaacagacg cccgccgcgc tgatcgtctt tgacagagtc
     3061 gtctcggccg acccgtcgtt caagaaatac tggctgcttc acagcatgga ggaacccgag
     3121 gttcgggagg aacgcaccat cgtgtctctc tccgagagag gctggagcgg ccggttgatc
     3181 aaccacacac tgctcccgga gccggtggat gccgagttcg tcaaggtcgg cgggccgggc
     3241 aaggagttct gggtcttcgg tgagaatttc cctaacgaca tctcgcgcgg cgacccgaac
     3301 acatatgaac tcggcgcctg gcgcgtggag ctctcgccga agagaccctc gtccgaggac
     3361 ctgttcctca acgtcattca gatcacggat cgcgagagaa attccgtgct gccggtcgcc
     3421 aaggtcgagg ccggccctat ggtcggcgtg cggattgcgg accgtctcgt gctctttgcc
     3481 cgcaccggcc gcccggctac caggcccgtc gccttcaccg ccaccggaga ggacacgctg
     3541 aagtacttgg tcaccgatct ctcggaggga acgtggcaag tctggcgtga cggacgcatc
     3601 cttcacccgg caatccacgt gaccggcagc gcaggatcgt tgtactttga gggcccgccg
     3661 ggagagtatt cgctccggcg ctgacgccgc ctcgggctac aatcgggagc gtgcatatga
     3721 accggcgtca gtttctccac acgagcctcg gcacggtcgc cgggctggtc ctcccccgct
     3781 ctgccctgcc tggcaacccg cccgacctga agatcaccgg gatcggattt ctccacctcc
     3841 agtttcccgg acgcacgcca cgccagcgca actcgatcat cgaaagcggc ggtgggccgc
     3901 ctggcatgtc gcagctcgag atctacaccg accagggcat cactggccgc agcctgtccg
     3961 gccggaacag catcatcgag aaccagcttt ttgcgcgcat caagggagag aacccctttt
     4021 acatcgagcg catctgggac cggatgtacc ggcacaaccg caagccggtc gccaagggtg
     4081 aatacattcg cgcgatgggc gccgtcgata tcgccatttg ggacattatc ggcaaggcgc
     4141 tcaatcttcc ggtctacaag gtgctcggcg catacaacga gaagctccgc gtctacgcgg
     4201 ccggcggtta ctaccaggaa gggaagggct tgaaggagct ggctgccgag atggagggat
     4261 acgtcggcga aggcttccgc gccgtcaaga tgaaggtcgg ctgggggccg atgcggcagg
     4321 acgccgagag ggtgcgcgcc gtccgtgacg ccgtcggtcc ggacatcgac gttctgctcg
     4381 acgccaacaa cgcctggaa
//
LOCUS       DATIFQ010000067         4071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1554067, whole genome shotgun sequence.
ACCESSION   DATIFQ010000067 DATIFQ010000000
VERSION     DATIFQ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554067"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2138)
                     /locus_tag="VLE22_02925"
     CDS             complement(<1..2138)
                     /locus_tag="VLE22_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB13383.1"
                     /translation="MFGSRTWRRSLPCGLLTLVSISTLVVSLGMAADPTGTITGIVTD
                     PSGSAVPDAQIAVKQTLTGLTRTLSTGADGAFLFPLMPVGTYEISVEAQGFRRFEQRG
                     ITLQVNAVANLPVTLQIGTLTESVSVQADAAMVETRSGTIKGVVDQQRIVELPLNGRN
                     AANLVLLVPGTVDLMAGNSRGSGDAIQGGTYPGGQAVASNGARSDGLNYLLDGGSNRD
                     PYTNVNDPFPNPDALQEFVVQTNNYGAEYGRASGAVVSVVTKSGTNEFHGGAFEFVRN
                     EKFNARNFFSPKADILKRNQFGGSLGGPVVRDKVFFFGTIQGTTLRALPASLNSVVPT
                     AAQRAGDFSSISRQLKDPSTGVPFADNQIPTSMFAQASVKLLDSIPVPSGPDGVIFYT
                     QPDVQHEVQFMGRGDYNTNRNRLYGRYLISDLPQEPTAIASNLVAARNGKGYRAQSVS
                     VSDTYTFSPSIVNSAVFTVGRNRATVISAAPFSFADLGVPIAATEPAELVLSVAGYFS
                     INTGHPGLFARQSYNFSDTIHWIHGRHELAMGFDYLKMNIDLDNTYRQNGNFRFRGTG
                     FSGNALSDFFLGKVDRFIQGGGEYAARRGTLASGFVQDNFRVNRRLTLNLGLRYDPFV
                     PFGDELGRTECFRPGQKSTRFTNAPVGYLYAGDSGCPEGGSDSHYLLFAPRFGFSYDV
                     TGQGRTILRGGYGIFYQPPFVEAFNNMVDSA"
     gene            complement(2301..3671)
                     /locus_tag="VLE22_02930"
     CDS             complement(2301..3671)
                     /locus_tag="VLE22_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB13384.1"
                     /translation="MNKTFSGKSGDKKSTRRAFLGRAAAGSVVLSTTARSYARIVGAN
                     DRIQIGQVGCGHRAFGLRRMLKLSSKTDPNFDFRSVCDIWSVNAQRAADHATQLFGKR
                     PKIYRHSEEMLADPELDAVMIATGDHQHARMLVEVVRAGKDCYCEKPMANTLADAKLA
                     RDTVKASKQILQMGSQWLSDPYQHRVREIVRSGKLGKITRIEQSWNFNGPRWRVPMDP
                     NIADVREKDTDWNRWLAGREPRPFDPRAYFEFRIYKDFSGGITDQWYSHGSGLAHFYL
                     DTFIPDNTVANGGIFAWHDGRENPDTFACLSIFAGKEVLYSFSVCFGNSYGDHTIIRG
                     QKGTLYSPGGEGSPQWWYLPERHSGWRSNVVYDLKLPGAPKPELVTVPGHPETPPIEQ
                     GDDLAYHTDNWLACMRSRKTPNGNIDTGFAHSVAVVMATRAYREEKKMYWNRKSEEIV
                     DHRPAA"
     gene            complement(3741..>4071)
                     /locus_tag="VLE22_02935"
     CDS             complement(3741..>4071)
                     /locus_tag="VLE22_02935"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13385.1"
                     /translation="GTKGTLFVDRTEYRVVPEPGSELAAVNEKASNNTNMAHWANFLE
                     CVKTRQKPISDIETGHRSTATCLLGNVALRSGLRIDWDDKTQTARQKQAARYLKREYR
                     KPWKLSV"
BASE COUNT          788 a         1183 c         1265 g          835 t
ORIGIN      
        1 gcgctatcca ccatgttgtt gaacgcctcg acgaacgggg gctggtagaa gatgccgtaa
       61 ccgccacgga ggatggtgcg gccctggcca gtcacatcat acgagaaacc gaagcgcggg
      121 gcgaatagca agtagtggga atcgctcccc ccttcgggac atccggaatc gcctgcatac
      181 aggtagccga ccggtgcgtt cgtaaagcga gtggacttct gaccgggccg gaagcactcg
      241 gtccggccca gttcgtcgcc gaatggtaca aacgggtcgt agcggagacc caggttcagc
      301 gtgagccgcc gattgacacg gaagttgtcc tgaacgaatc cgctggccag cgtgccgcgc
      361 cgcgccgcgt attcgccgcc accctgaatg aaacggtcga ccttgccgag gaagaagtcg
      421 gagagggcgt tgcccgaaaa acccgtgccg cggaaccgga agttcccgtt ttgccggtac
      481 gtgttgtcca ggtcaatgtt catcttgagg tagtcgaagc ccatcgccag ttcatggcgg
      541 ccgtgaatcc agtgaatcgt gtcactgaag ttgtaactct gccgggcgaa caggcccggg
      601 tggcccgtgt tgatgctgaa atagcccgct acggagagga ccaactcggc tggctctgtg
      661 gctgcaatcg gcacgccaag atcggcaaaa ctgaagggcg cggccgagat tacggtcgct
      721 cggtttcggc cgaccgtgaa caccgcgctg ttgactatgc tcggactgaa agtgtaggta
      781 tcgctcacgc tcacggactg agcgcggtaa cccttcccgt tgcgtgcagc caccagattg
      841 ctcgcgatcg cagtgggctc ctggggcagg tccgagatca aatagcggcc atagagacga
      901 tttcggttgg tgttatagtc ccctcgtccc atgaactgga cctcatgctg aacgtcgggt
      961 tgcgtgtaga agatgactcc gtcgggaccg gagggaaccg ggattgaatc gagcagcttg
     1021 acagatgctt gagcaaacat gctggtggga atctggttgt ctgcgaaagg aacgcccgtc
     1081 gacgggtctt tcagttggcg tgagatcgaa gagaaatcgc ccgcccgctg ggcggcggtg
     1141 ggcacgaccg aattcagcga cgcaggaagc gcacggagag tggtcccttg aatcgtaccg
     1201 aaaaagaaga ccttatccct gaccaccggc cccccgagac taccgccgaa ctgattgcgt
     1261 ttcagaatgt ccgctttcgg agagaagaag tttctggcgt tgaatttctc gttccggacg
     1321 aactcaaagg cgcccccgtg gaactcgttg gtgcctgatt tcgtgactac gctgacaacg
     1381 gcgcctgacg cgcggccata ttcggcgccg tagttgttcg tctgcaccac gaactcctgg
     1441 agagcgtcag ggttggggaa cggatcattg acgttggtat aggggtcccg gttgctgccg
     1501 ccgtcaagga gatagttgag gccgtcgctg cgagccccat tagacgccac ggcctggccg
     1561 cctgggtagg tgccgccttg aatggcgtcg ccagaccctc gcgaattgcc cgccatcagg
     1621 tccacggttc cagggacaag gagtacgagg ttcgccgcgt ttcggccgtt caagggtaat
     1681 tcaacgatgc gctgctggtc caccacgccc ttaatcgtgc ccgatcgcgt ctcgaccatt
     1741 gctgcgtcgg cctgcacact gacagattca gtgagggtac cgatctgaag agtgaccggg
     1801 aggttggcaa cggcattcac ctggagggtg ataccccgct gctcgaaacg ccggaaaccc
     1861 tgcgcctcga cactgatttc atacgtgcca acgggcatca aggggaagag aaaagctcca
     1921 tcggcgccag tgctcaatgt gcgggtaagg ccggtcagag tctgctttac ggcgatctga
     1981 gcgtcaggaa cggcgctgcc tgaggggtct gtcacgatac ctgtgatggt cccggtggga
     2041 tcggctgcca tcccaaggga gacgaccaag gtagagatcg acacgagggt cagtaggccg
     2101 cacggaaggc tccgccgcca ggttcggctg ccaaacatgg cacacctcct taatgagtga
     2161 taactattgc ttaatggaat gaagtaactg tctgcctacc ccactttggt acgattttgc
     2221 ggcaactcgt acagaatgtc aatgcgcgcg ttcgcatccc atccagacag ttggacactg
     2281 tggaagccca gcgtgagaag ctaagcggcg ggccggtggt cgacgatctc ttcgctcttc
     2341 cggttccagt acatcttctt ctcttcgcga taggcgcggg tggccataac aaccgccacg
     2401 gaatgggcga agccggtgtc gatgttcccg ttgggagtct tgcggctgcg catgcaggcg
     2461 agccagttgt cggtgtgata ggcgaggtca tcaccctgct caatcggcgg tgtttccgga
     2521 tgtcccggta cggtgaccaa ctcgggcttc ggagcgccgg gtaacttcag gtcgtacaca
     2581 acattcgaac gccagccgct gtgtctctcc ggaagatacc accactgcgg gctgccttcg
     2641 cctcccggag agtagagcgt acccttctgg ccacgaatga tggtgtgatc tccgtacgag
     2701 ttcccgaagc acacgctaaa gctgtaaagc acttctttcc cggcgaatat cgacaggcag
     2761 gcgaaggtat caggattctc ccggccatcg tgccaggcga agatgccgcc gttggcgact
     2821 gtgttgtcgg ggatgaaagt gtccagatag aaatgggcga ggcccgaacc gtggctgtac
     2881 cactggtccg tgatgccgcc ggagaagtcc ttatagatgc ggaactcgaa gtaagcgcgc
     2941 gggtcgaacg ggcggggctc cctgcccgcc aaccagcggt tccagtcggt gtctttctca
     3001 cggacgtcgg cgatgttggg gtccatcggg acacgccagc ggggcccgtt gaagttccag
     3061 ctctgctcaa tccgtgtaat ctttcccagc tttccgctgc gaacgatctc ccggacgcgg
     3121 tgctggtaag gatcactcag ccactgtgaa cccatctgca agatctgctt actcgccttg
     3181 acggtgtcgc gggcgagttt ggcatccgcg agcgtgttgg ccatcggctt ttcgcagtag
     3241 cagtccttgc cggcccgaac aacttcgacc agcatccggg cgtgttgatg gtctccggtg
     3301 gcgatcatca cggcatccag ctccgggtcc gccagcattt cctcggagtg cctgtaaatc
     3361 ttcggacgct tgccgaacaa ctgcgttgcg tgatcggcgg ctctctgcgc gttcaccgac
     3421 cagatgtcac aaacgctgcg aaaatcgaaa ttcgggtcgg tcttggatga cagtttgagc
     3481 atccggcgaa gcccgaaggc tcgatgcccg catccgacct ggccgatctg aatacggtcg
     3541 ttggcgccga cgattctggc gtaggagcga gcggtcgttg agagaacgac ggatcccgcc
     3601 gccgctcttc cgagaaaggc tctccgcgtg gacttcttgt ctccggattt cccgctgaat
     3661 gtcttgttca ccggtctcgt acctcgatct gatcatagcg cccggaggac ggcctggcgg
     3721 ccagccatga gtttcacaac ctatacactc agcttccagg gcttccggta ctcgcgcttg
     3781 agataccggg cagcctgctt ctggcgcgcc gtctgggttt tatcgtccca gtcgatacgc
     3841 aggccgctgc ggagcgcgac gttgccaagc aggcacgtcg ccgttgagcg gtggccggtc
     3901 tcaatgtcgc tgatcggctt ctgccgggtt ttcacgcact ccaggaagtt ggcccagtga
     3961 gccatgttgg tgttgttgga ggccttctcg ttcaccgccg ccagctcgga gcccggctca
     4021 ggcacgaccc ggtattcggt acggtccaca aacagggttc ccttggtgcc g
//
LOCUS       DATIFQ010000068        16386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_789023, whole genome shotgun sequence.
ACCESSION   DATIFQ010000068 DATIFQ010000000
VERSION     DATIFQ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16386
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789023"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..2186)
                     /locus_tag="VLE22_02940"
     CDS             complement(276..2186)
                     /locus_tag="VLE22_02940"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSB13386.1"
                     /translation="MTPRLVAVAGPLTGAVFPVADADFSIGREESNSLCLEDRAVSHH
                     HCVVRAEGERFKITDLGSVNRTCVNGIPVSERFLAHGDEIKVGHSLFLFLLEEEPRAR
                     AEPVQLDEGALASGATVELRSDDALYLSPEKVLEALSEKGRVARDLGVLLGIGTAISS
                     VRGLENLERRLMELTFEAIPADHGAILLIETSLGELESAFHWSRVSRARQGFPISRTV
                     ASRVFEQGVAILCNDVRSAPGLSTAESVLRAHASSILAAPLACFEKVLGILYLVSCDP
                     AVRFDENHLHLATGIAGIAAGALDSALRMEELNDENRRLKAEIGVEHGLVGDSQRLRQ
                     VYEFIAKAAPGASTVLIRGESGTGKELVARAIHRNSPRAAKPFVAINCAALTESLLES
                     ELFGHEKGAFTGAIIQKRGKLEEAHGGTVFLDEVGELAHVLQAKLLRVLQEREFERVG
                     GTRPIKTDIRLIAATNRDLEEAVKAGQFRQDLYFRLNVVSVTMPPLRDRRGDIPLLAS
                     FFLQKHGKSARRRIAGISDEALSLLMSYQWPGNVRELENAIERAVVLGSTEIIQPDDL
                     PDRVLEADRPAGASSLQYHEAMKEAKRQLILRTLEQAGGSFTEAAKVLGLHPNNLHRL
                     VRTLNLRAALKK"
     gene            2275..3504
                     /locus_tag="VLE22_02945"
     CDS             2275..3504
                     /locus_tag="VLE22_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB13387.1"
                     /translation="MTPRERVLTTLNHREPDRVPVDFSGHRSSGIAAIAYHRLRAALG
                     LPPAEARVYDPVQQLAIVHDDVLDRFGGDTIEMGRGFSLEAADWADWTLPDGSPCQMP
                     VWALPVRGDGEWVLNSPAGRTLARMPDGALFFEQCHWPFLDTDDLGRLPEAFSESMWT
                     GLRSPPGPAAADPAALAEGARRLREKTDRAIVGLFGGNLFELGQFLYRMDNFLMMLAG
                     EPLRAHAFLDKATEVHLDNLERYLETVGPYIDIIQFGDDLGSQRGPLLSPKMYREFFK
                     PRHQFLWKYAKKLANVKVMLHCCGAVRPLLPDLIDAGLDAINPVQIAGKDMDAAGLKR
                     DFGNDITFWGGGCDTQKTLPDGTPDEVRAHVLGQLEVLAPGGGFVFQQVHNIMANVPP
                     ANIIAMFDAIREFNGRC"
     gene            complement(3514..4206)
                     /locus_tag="VLE22_02950"
     CDS             complement(3514..4206)
                     /locus_tag="VLE22_02950"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HSB13388.1"
                     /translation="MKRLVPLVFAVGLLSCAQDEYQAQRERMVREQMESRDIQNREVL
                     RAMRAVPRHLFVPPNLRSMAYADHPVPIGYSQTISQPYIVALMTQMLEPEKGHKALEI
                     GTGSGYQAAVLSPLVKQVYTIEIVPELARSAGALLMQLGCKNVTVKQGDGYRGWPEEA
                     PFDRIILTAAPPEVPQALLDQLKPGGRLVAPVGGSVFDQELVVIVKGADGKVRREPGI
                     PVRFVPMVRGEK"
     gene            4235..5656
                     /locus_tag="VLE22_02955"
     CDS             4235..5656
                     /locus_tag="VLE22_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB13389.1"
                     /translation="MSKVAQRKSTSPPVVADSPAALLRQLGIVSATALVVSNMVGTGI
                     FATSGFLAGDLGDPMLVLVIWLVGALFALIGAFCYSELGINFPASGGEYVYLTRAYGP
                     TWGFMTGWVSFFAGFSAPIAAAALAFSDYLGYFFPVLKQENAHFTIGSGAFSLTLGGA
                     QIAASTLVAVFTLLNFFGVRRVARVQNVLTATKVLVLVAFIGLGFFIGTGNWDHFSMP
                     AVRTSATPLAAQFAISLFWIYVSYSGWNAATYVAEELKRPERTLPAALAVGTALVAAL
                     YIGLNLIFIYATPLEEMKGVVAVGALAASKLFGPGIAGTFSALMALSLVSTVNAMVTI
                     GPRVYYAMAKNGAFLPVAAKVDPRWHTPVPAILLQGLCTILMTLTPFPALVVYIGFTL
                     NFFAVMSVTSLFKFRRRPEWQRLRIVSFAFPLLPALFVLIGAWMTVYGMILKPNVSLA
                     AILTIATGAAVYHFRFAPRTTRD"
     gene            5744..6664
                     /gene="folD"
                     /locus_tag="VLE22_02960"
     CDS             5744..6664
                     /gene="folD"
                     /locus_tag="VLE22_02960"
                     /inference="COORDINATES: protein motif:HMM:NF010783.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HSB13390.1"
                     /translation="MAQILDGNRIRDEIQAELKPRVSRLAASCRPPGLAVVLVGDDPG
                     SQIYVRNKVKACEELGIYSEKLTPPDTITTEELATLVEQLNARDEIDGILVQMPLPRQ
                     VDAKKVLFAVSPEKDVDGFHPLNVGNLVANRPGPRSCTPAGILEILKRYRIPIAGRQA
                     VVVGRSDIVGKPVAFLLLHEHATVTICHSKTFDLPGVCRRADILVAAMGRAALITRDY
                     IKPGATVIDVGINRIDDRSRVETIFRGDPAKLAAFDRKGSVLVGDVHPLDVAEVAGAY
                     TPVPGGVGPLTIAMLMVNTVQIAERRTGGC"
     gene            6658..7278
                     /gene="coaE"
                     /locus_tag="VLE22_02965"
     CDS             6658..7278
                     /gene="coaE"
                     /locus_tag="VLE22_02965"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HSB13391.1"
                     /translation="MLKVGLTGGLATGKSFVGAALADMGCHLIKADELGHQVLLPGGE
                     AYDGVVREFGAGILNPDGTIDRKRLASEVFGNPERLAALTRLVHPPVIRREEEFIAKI
                     AAREPDAIAVVEAAILIETGSYKRFDTIILAVCGEQQQVERAVLRDGADPDEIRARIR
                     RQMPLEEKRKYATYIIDTSGSKEDTLRQTRAVYNLLRSIPQCSSAS"
     gene            7260..8405
                     /locus_tag="VLE22_02970"
     CDS             7260..8405
                     /locus_tag="VLE22_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSB13392.1"
                     /translation="MFKRILILAAVILGGFYLLTSVTHWNPRDALAPVRDAGGLWTQP
                     DHARSAGLSADELNNIEVYKTAHNATVNITTVVYREDWFFRLVPVEGSGSGFIVNAEG
                     QIVTNNHVVSGGRQIKVILADKSTYEARVLFNDEANDLALLKITPRKKLPFLRLGDSD
                     QLQVGQKVLAIGNPFGLEGTLTTGVISSLNRTIQAEANSPMEDMIQTDAAINPGNSGG
                     PLLDTQGNVIGINTMIVGAANIGIGFAIPINRAKWRLDDYQARGRYARPYLGIRSLYV
                     SGELAEALDLPAEGGLLVISAERSSPAESAGLRGARRRVVVGNYEIPVGGDLIMAVDG
                     QAVDGNTTLERAKNRKRVGEALNLTIYRDGRTMKVKVTLGEAPEQRL"
     gene            8484..9683
                     /locus_tag="VLE22_02975"
     CDS             8484..9683
                     /locus_tag="VLE22_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB13393.1"
                     /translation="MVLVQRVSKVYPLYRRPSDRILELFPFRRTPLHSDFWALRDISF
                     SVERGEFLGIVGPNGSGKSTLLQILSGILQPTSGRVVTEGRIAALLELGAGFNPEFTG
                     RENVYINGEIMGLSRREIERAFPKIEAFAEIGEFIHRPVKEYSSGMYVRLAFSTAIHV
                     DPEILIVDEALAVGDAIFANRCIQKFEELKDRKVTVLFVSHDLGLVKRLSNRAILMVQ
                     GQVVAEGNPSDVVNRYVGLVHKRGRLEEAREGRALTASYRHGDGASRVVEVELLNEAG
                     EPVSTIQSGEQVTVRARAKFLRPVEEPIVGILIRNRLGTDVFGTNTKLEKRKLGMFAP
                     GDLLTAEFRFNCLLSQQEYTLTVATQYWDGSSQDWLDDVLGFRVVDEKAAAGLLSLKT
                     EIEWRKE"
     gene            9668..11161
                     /locus_tag="VLE22_02980"
     CDS             9668..11161
                     /locus_tag="VLE22_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HSB13394.1"
                     /translation="MAERIAPAVDGQAARFFGHPRGLATLFLTEFWERFSYYGTRALL
                     ILFMTAPAATGGLGFDVGKAGAIYGLYTSACYLMSLPGGWLADQFFGQRRAVLYGGSV
                     MALGNLLLAAPNVALFYPGLALVALGTGLLKPNVSVMVGQLYADSDARRDAGFSIFYM
                     GINLGAFVSPLACGYLGQRVDWHLGFALAGFGMIAGLIQYVAGGKHLGKAGLKPAPVN
                     AEESKRQLRKLRIVLLAAGVMFGGAVAFQAAGVMRITAQALSDALGWILVLIVAAFFV
                     WALFLGDWSAVERKRLVVVGVLFLASSLFWCMFEQAGSTLNLFAERNTDKTVLSFAVP
                     ASWLQALNALFIILLAPVFAWIWIKLGRREPSSPTKFVLGLVAGGLGYAVLVVGASVA
                     AQGVQVSLMWLVVVYLFHTVGELCLSPVGLSAMTKLAPAKISGLLMGVWFLSISVGNY
                     MGGRLASLYESLPLTELFGAVAAFGIGAGLVLAVFVKPVKRLMGGVK"
     gene            11176..12345
                     /locus_tag="VLE22_02985"
     CDS             11176..12345
                     /locus_tag="VLE22_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HSB13395.1"
                     /translation="MKLDEIQAALREQGLDGWLFFDHHQRDPLAYRVLEFAPEGPVTR
                     RWYYLIPADGEPRKLVHAIESFVLDALPGARLVYSGWARQTEGLLELLAGCRRVAMQY
                     SPQCAIPYVSMVDAGTVELVLATGVEITSSADLIQYFEARWDEEKLESHLEAGRRVDE
                     IRGNAFRLIGEKLRAGERIREFDVKQFILDSFAAAGMVTDHGPIVGVNANAANPHYEP
                     SADASEEIRRGDLVLIDMWAKLAKPRSVYYDITWTGFCGTEPRAEIERVFGIVREARD
                     RAVRRVRKAVEEGEPLRGFEVDDAAREYIREQGFADQFVHRTGHSIGEDVHGAGANMD
                     NLETHDERRVIAGTCFSVEPGIYLRDFGIRSEVNVYVGERSARVTGEAQERLVLI"
     gene            12345..13964
                     /locus_tag="VLE22_02990"
     CDS             12345..13964
                     /locus_tag="VLE22_02990"
                     /inference="COORDINATES: protein motif:HMM:NF044456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13396.1"
                     /translation="MRRVAFLALAMAGACVAVDSHPKAFPLSAQDGVALIEWVSASTF
                     RFCRAWETASCKPGVNREVEVEREDAGARLRFRTKYLHVDVDKATLRVSARTPEGPEL
                     LEDLTEARRAEGAVTIERSAPVGERFYGLGAHAYESADLRGTAMDTTKPFLISSRGYG
                     LHHVSPGRYTFDLGRKHSDRYRITVRGARKIDYYFHFGPSPKEILEEHKEVAGTPDIL
                     KEWQLGIIAQTQLPAYATPLPATLDASWESLCDCVRMMSHASLSGIPLPAFDIGLYRS
                     NSLALYRRALQLAQVAPVVFSSSAASADAEMLVMDESAAQARRRWLPFFLTYADEARL
                     RGLPMLHSLPLQFPRDHEASKRADEFLMGDEMLAAPVCTEDGRRSVYLPMGVWTNWRT
                     NKVYPGRQTIEIEAAEDELPLFVKNGTIVPAGPRSGSAPMELHYFPKLASEFFLFEPE
                     VSDYTQAHANPALDLWRLEIAPKVDRTYEWVLHNLPAPTRVWEGETEYQRVQDRGLLK
                     PGCWYFDEVTSNIHVTVRTLKGATRIVHMGQ"
     gene            complement(13995..14360)
                     /locus_tag="VLE22_02995"
     CDS             complement(13995..14360)
                     /locus_tag="VLE22_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13397.1"
                     /translation="MTRLGWKTAAAVIPYRLINSASFTFYSAGWVILMLMVFYWLVEV
                     LGYRRWAYPFIVLGMNSIFIYSFSQILHGWLYQGLGVFTGNFWYLGRLGEIPQNLATL
                     GVMWYLCYWLYQRRIFLKI"
     gene            14834..15358
                     /locus_tag="VLE22_03000"
     CDS             14834..15358
                     /locus_tag="VLE22_03000"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:15035..15037,aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSB13398.1"
                     /translation="MRAISAFAVCSVLFTGLTVQGATHLKVGDTAPDFTLPSSTGKPV
                     SLSEFRGKKGVVLAFFPAAFTGGUTREITAYQSGIAGFEGMDVQVLGISTDGQFTLKH
                     WADEHLKTTFPLLSDFLRKTSESYGVLNAESGMANRTTFVIDTEGRIQHIEEGKSAID
                     PSGAMTACSRVRKK"
     gene            complement(15367..>16386)
                     /locus_tag="VLE22_03005"
     CDS             complement(15367..>16386)
                     /locus_tag="VLE22_03005"
                     /inference="COORDINATES: protein motif:HMM:NF016563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HSB13399.1"
                     /translation="GVMEGEQHNTYDIEFYGPNTMMGTFYLGALAAASKMAVHLGDNA
                     AAERYRKLMEEGAARLDKLLWNGEYYVQKIDERAEKASRYQYGEGCLSDQLLGQWFAE
                     VVGLGKLLPHEHIQSTLRAIFRHNFREDFSDFPNAQRLYALNDEAGLLLCSWPRGHRP
                     ALPFVYSDEVWTGIEYQVAAHLFYEGMFKEGLAIVDAVRNRYDGVRRNPWNEIECGHH
                     YARAMSSWSLVLALSGFQWSSSEKALRFSPLVSQDNFRSLYSTGTAWGNYSQKRTKQK
                     LDAEITVEGGALELAQLRLPFPGKPSGSSLAGATVEAAGGEAIIRPKKPLRLGKGDRL
                     AVSVG"
BASE COUNT         3020 a         4989 c         5215 g         3162 t
ORIGIN      
        1 gttcggggtt gctgtcggtc gcctcgcgga tgttgttttc tatcgggtgc aagacctttc
       61 tggtcaactg gatatccccg agtcgttagt actcggccac attatggcgc acgaactcgg
      121 ccatgtgctg cttggtccgg aaagccactc ttcgagcgga gttatgatag cccacctgag
      181 gcggcgcgat ctgccgacag tggtcaaagg gtggctggtg ttcaactccc agcaggcagc
      241 gcggatgtac gcccacctgc ggcaacccgc cgtggctatt tcttcagcgc cgcccggagg
      301 ttcagtgtcc gcacgagcct gtgcagattg tttggatgca gccccagcac cttcgccgct
      361 tcggtgaagc tgccgccggc ctgctccagg gttcgcagga tgagctgccg cttggcttcc
      421 ttcatggcct cgtggtattg cagcgaggag gcgccggcgg ggcggtccgc ttcgaggacg
      481 cggtccggaa gatcgtccgg ctggataatt tcggtcgaac cgaggaccac ggcccgctcg
      541 atggcgttct ccagctcgcg aacgttgccc ggccattggt aactcatcag aagcgacagc
      601 gcctcgtcgg agatgccggc gattcgccgc cttgcgctct tgccgtgctt ctgcaagaag
      661 aagctcgcca gcagcgggat gtctccgcgc cgatcacgta gtggcggcat cgtgaccgaa
      721 accacgttga gccggaagta gaggtcctgg cggaactgcc cggccttgac cgcttcctcc
      781 agatcccggt ttgtcgccgc gatgagacgg atgtccgtct tgatcggccg ggtgccgccg
      841 acccgctcga actcacgctc ctgcaataca cgcagcagct tcgcctgaag aacgtgcgcc
      901 agttcgccga cctcgtcaag gaaaacagtc ccgccgtgcg cctcctccag ctttcctctc
      961 ttttgaatga tcgcgccggt gaatgcgcct ttctcgtggc cgaacagctc gctttcgagc
     1021 aggctctccg tcaaggcggc gcagttgatc gctacgaacg gtttggcggc acgcgggctg
     1081 ttgcggtgaa tggcgcgggc cacgagttcc tttcctgtgc cgctctcgcc gcggatcagg
     1141 acggtggagg ccccgggcgc ggccttggcg atgaactcgt agacctgccg caggcgttga
     1201 ctgtcgccca ccaggccgtg ctcgacgccg atctcggcct tgaggcgccg gttttcgtcg
     1261 ttcagttcct ccatgcgcag cgcgctgtcg agagcgccgg ccgcgatccc cgcgatcccc
     1321 gtcgccaggt ggaggtgatt ctcatcgaag cgcacggccg ggtcgcatga gacgaggtag
     1381 aggattccca gaaccttctc gaaacatgca agcggggccg cgagaatgga gcttgcatgc
     1441 gcacggagca cgctctcggc cgtgctcaag cccggagcgg agcggacatc gttgcacaag
     1501 atcgcgactc cttgctcgaa aacccggctc gcgaccgtcc gcgatatggg gaatccctgt
     1561 cgggcgcgcg aaaccctgct ccagtggaag gccgattcca gctcgcccag gctcgtttca
     1621 atcagcagaa ttgctccgtg atcggcggga atcgcctcga aagtcaactc catgagccgc
     1681 cgctccaagt tctccagccc gcggaccgaa gagatcgccg tgccgatccc caggaggact
     1741 ccaagatccc tagcaacgcg tcccttttcc gagagggctt cgagaacttt ttccggactg
     1801 aggtagagag cgtcgtcact ccgaagctcc accgtcgcgc cggaggccaa tgccccttcg
     1861 tcaagttgca ccggctcggc cctggcgcgc ggttcttctt cgagaagaaa caggaacagg
     1921 gagtgcccga ccttgatttc gtcgccgtgc gcgaggaaac gctccgagac agggatgccg
     1981 ttgacgcagg tgcgatttac gctgcccaga tcggtgatct tgaaccgctc tccttccgcc
     2041 cggacgacgc aatgatggtg cgaaaccgcg cggtcctcga gacaaaggga attggattct
     2101 tcgcgtccga tcgagaagtc tgcgtctgcc acgggaaaaa cagcgcccgt aagcggcccg
     2161 gcgacggcaa ctaatctcgg cgtcatgaca cggcaatcca cgctgattct acaacctctc
     2221 aactgtgata ggcgaggcta taatggcggt ctgcctcggg aggtgaattc tttcatgacg
     2281 ccgcgcgaac gcgtgctcac cactctcaat catcgcgagc ccgatcgggt tcccgtcgac
     2341 ttctccggcc accgttcgtc cggaatcgct gctatcgcgt accaccggct ccgtgccgcc
     2401 ctcgggctgc cgccggcgga ggcgcgcgtc tatgatccgg tacagcaact ggccatcgtg
     2461 catgacgacg ttctcgatcg cttcggtggt gacaccatcg agatgggccg gggtttctcg
     2521 ctcgaggccg cagactgggc cgattggacc ctacccgacg gatcgccgtg ccagatgccg
     2581 gtctgggcgc tccccgtacg cggcgacggc gagtgggtgc tgaattcgcc cgccggccgg
     2641 actctcgccc gtatgcccga cggcgccctg ttcttcgaac agtgccactg gccttttctc
     2701 gataccgacg acctggggcg gctcccagag gcattcagcg agtcgatgtg gaccggcctg
     2761 cggagtccgc ccggtcccgc cgcggccgat ccggcggcgc tggcggaagg cgcccgccgc
     2821 cttcgcgaga agaccgaccg tgccatcgtc ggcctcttcg gaggcaacct gttcgagctt
     2881 ggccagttcc tttaccggat ggacaacttc ctcatgatgc tcgccggcga accgctccgc
     2941 gcacacgcct ttctcgacaa ggcgaccgag gtccatcttg acaatctcga acgttacctg
     3001 gagacggtcg gcccctacat tgacatcatc cagttcggtg atgatctcgg ttcccagcgt
     3061 gggcccctcc tctcgccgaa gatgtatcgc gagttcttca aaccccggca ccagttcctg
     3121 tggaagtatg ccaaaaagct ggcgaacgtt aaggtcatgc tgcattgctg cggcgcggtg
     3181 cgccccttgc tgccggacct catcgacgcc ggcctcgatg caatcaaccc ggtccagatt
     3241 gcgggcaagg acatggatgc tgcgggcctg aagcgcgact tcggcaacga catcaccttc
     3301 tggggcggag gctgcgacac ccagaagaca ctgccggacg ggacgcccga cgaagtgcgg
     3361 gctcacgtgc tcggacagct cgaagtgctg gcgcccggag gcggcttcgt ctttcagcag
     3421 gtccacaaca tcatggcgaa tgttccgccg gcgaacatca tcgccatgtt tgacgcgatc
     3481 agggagttca acggccgctg ctgagccggg aacctacttc tcgcctcgca ccatcggcac
     3541 gaagcggacc ggaattccgg gctcccggcg caccttccca tccgccccct taacgatcac
     3601 gaccagttcc tggtcgaata ccgaaccgcc caccggcgct accagcctgc cccccggctt
     3661 gagctgatcg agcagtgcct ggggcacttc cggtggagcc gctgtcagga tgatccgatc
     3721 gaacggcgcc tcctccggcc atccgcggta cccgtctccc tgtttcactg tcacattttt
     3781 gcaacctagt tgcataagta gtgcgcccgc cgaccgggcc agctctggta cgatctcgat
     3841 cgtgtacacc tgcttcacca gcggcgacag cacggccgcc tggtagcccg acccggtgcc
     3901 gatctcgagc gccttatggc ccttttccgg ctccagcatc tgggtcatca gggcgacgat
     3961 atagggctgg gagatcgtct ggctgtagcc aattggcacc ggatggtccg cgtacgccat
     4021 cgaccggagg ttggggggga cgaacaggtg ccgcggcacc gcccgcatcg cccgcagaac
     4081 ttcgcggttc tgaatgtcac ggctttccat ctgttcccgc accatccgct cccgctgcgc
     4141 ctgatactcg tcctgagcgc acgaaagcag gccaaccgcg aaaacaagtg gaaccaaccg
     4201 tttcatatca gcatcgtagc tgtgctggcg tacaatgtcc aaggtggcgc agcgaaaatc
     4261 aacttcccct cctgttgtgg cggacagccc cgccgctcta ctccggcagc ttggcatcgt
     4321 cagcgccacg gcgctggtgg tgtcgaacat ggtcggtacc ggcatctttg cgaccagcgg
     4381 gtttcttgcc ggcgatctcg gcgaccccat gctggtgctc gtaatttggc ttgtcggcgc
     4441 tctgttcgcg ctcatcggtg cgttctgtta ctccgagctc ggcatcaact ttcccgcctc
     4501 cggcggcgag tacgtctatc tcacgcgcgc gtacggcccc acctggggct tcatgaccgg
     4561 atgggtgtct tttttcgccg gcttttcggc gccgatcgcc gcggcggctc tcgcattctc
     4621 ggactatctt ggctacttct tcccggtgct gaagcaggag aacgcccact tcaccatcgg
     4681 ctccggcgcg ttttcgctta cgctcggcgg cgcgcagatt gctgcctcaa ccctggtcgc
     4741 tgttttcact ctgctcaact tcttcggcgt ccgaagagtc gccagagttc agaatgtgct
     4801 gaccgcgacc aaggtccttg tgctcgtcgc cttcatcggt cttggcttct tcatcggcac
     4861 cggcaactgg gatcacttct cgatgccagc cgtgcgcaca tccgcgacac cgctggcggc
     4921 tcaattcgcc attagcctct tctggatcta tgtgagctac agcggctgga acgccgccac
     4981 ctatgtcgcc gaagaactaa aacgtcccga gcgcaccctg ccggccgcac tggcagtcgg
     5041 taccgcgctc gtggcggctc tctacatcgg actgaacctc atattcatct acgccacgcc
     5101 gctcgaggag atgaaaggcg tggtcgcggt tggcgcgctt gcggcatcga agttgttcgg
     5161 ccccggcatc gcgggaacgt tcagcgcatt gatggcgctg tcgctcgtct cgaccgtgaa
     5221 cgccatggtg accatcggcc cgcgcgtcta ctacgcgatg gcgaaaaacg gagctttcct
     5281 gccggtggcc gcgaaagtgg atccccgctg gcatactccc gtgcccgcca tcctcctgca
     5341 agggctgtgc accatcctga tgacgctcac tccctttccg gcgctggtcg tctacatcgg
     5401 gtttacgctc aactttttcg cggtgatgtc ggtcacctcg ctgttcaaat tccgccggcg
     5461 tcccgagtgg cagaggctcc gcatcgtgag cttcgctttc ccgttgctgc ccgcgctatt
     5521 tgtgctgatc ggagcgtgga tgaccgtcta cggcatgata ctcaagccga acgtctcgct
     5581 tgccgccatc ctcaccatcg ccaccggcgc cgccgtgtat cacttccgct tcgccccccg
     5641 cacaacccgg gactgacacg aacgcaaaac ccgtcccgcg gttttgcctt tcccgggttt
     5701 tccttctcag tccccagccc ccgtctgcta tcctgcctgt aacatggcgc agattctgga
     5761 cggaaatcgg atccgcgatg aaattcaggc cgaattgaag ccccgcgttt cgcggctggc
     5821 cgcatcctgc cggccccccg gcctcgcggt cgtgctcgtc ggcgacgacc caggctctca
     5881 gatctacgtc cgcaacaaag tcaaggcctg cgaggaactt ggcatctata gcgagaagct
     5941 cactccgccc gacacgatca ccaccgaaga gctggccact ctcgtcgagc aactcaacgc
     6001 ccgcgacgag atcgacggca tcctcgtgca gatgccgctc ccccgccagg tggatgccaa
     6061 gaaagtcctc ttcgccgttt cgccggagaa ggacgtggat ggcttccatc ccctgaacgt
     6121 cggcaatctt gtggccaacc ggcccggccc ccgctcgtgc acccccgcag gcatcctcga
     6181 gatcctgaag cgctaccgca tccccatcgc cggccgtcag gcggtcgttg tcggacgcag
     6241 cgacatcgtc ggcaaaccgg ttgccttcct cctgttgcac gaacacgcca ccgtcaccat
     6301 ctgccactcg aagaccttcg acctgccggg cgtctgccgc cgggccgaca tcctcgtcgc
     6361 tgcgatgggc agggccgccc tcatcacccg ggattacatc aagcccggcg ccaccgtgat
     6421 cgacgtcggc atcaatcgca tcgacgatcg ctcacgcgtc gaaacgatct tccgcggcga
     6481 ccccgccaag ttggccgctt tcgaccgcaa gggcagcgtc ctcgtcggag acgtccatcc
     6541 gctcgacgtg gccgaggtcg caggcgccta cacccccgtg cccggcggcg tcggccctct
     6601 gaccattgcc atgctcatgg tcaacaccgt ccagattgcc gagcggagaa ctggcggatg
     6661 ctgaaggtgg gtctcaccgg agggctcgcc acaggcaaga gcttcgttgg tgcggccctc
     6721 gcggacatgg gctgccacct catcaaagcc gacgagctcg gccaccaggt tctgctcccc
     6781 ggcggcgaag cctacgacgg cgtcgtgcgc gagtttggcg caggcatact caacccggac
     6841 ggcacgattg accgcaagcg cctggcctct gaggtgttcg gtaatccgga gcggctggcc
     6901 gcgctcaccc gcctcgttca cccgcccgta attcgccgcg aggaggagtt catcgcgaag
     6961 attgccgcac gcgaacccga cgccatcgcc gtggtggaag ccgctatcct gatcgagacc
     7021 ggtagctata agcgcttcga cacgatcatc ctggctgtct gtggcgaaca gcaacaggtg
     7081 gagcgcgccg tgcttcgtga tggcgccgat cccgacgaaa tccgcgcccg cattcgacgc
     7141 caaatgccgc tcgaagagaa acggaaatac gccacctaca tcatcgatac atccggttcc
     7201 aaggaggata cgctccgcca gacccgggca gtctacaatc tccttaggag tatcccgcaa
     7261 tgttcaagcg catcttgatc ctcgctgccg tcattctagg cggattctac ctgctgacat
     7321 cggtcaccca ctggaatccg cgcgacgcgc tggcgcctgt tcgggatgct ggcggcctct
     7381 ggacccagcc ggaccatgct cgctccgccg ggcttagcgc cgacgaactg aataacatcg
     7441 aagtctacaa aacagcccac aatgcgaccg taaacatcac tacggtggtc tatcgggagg
     7501 actggttctt ccgcctcgta cccgttgaag ggtccggctc cggcttcatc gtgaacgcag
     7561 aaggccagat cgtcaccaac aaccacgtcg tctccggtgg ccgccaaatc aaggttatcc
     7621 tcgccgacaa aagcacctac gaggcccgag ttttgttcaa tgacgaggcc aacgacctcg
     7681 ctttactgaa gatcacaccc cggaagaagc tgccgttcct caggctcggt gattccgacc
     7741 agttgcaggt gggccagaag gtgctggcca tcggtaaccc tttcgggctc gaaggcacgc
     7801 tgaccacagg cgtgatcagc tctctcaacc gcaccatcca agccgaggcg aactccccga
     7861 tggaagacat gatccagacg gacgccgcca tcaatccggg taacagcggc gggccgctgc
     7921 ttgacaccca gggcaacgtg atcgggatca acacgatgat cgtcggcgcc gccaacatcg
     7981 gcattggctt cgccatcccg attaaccgcg ccaagtggcg cctcgacgat taccaggccc
     8041 gcggacggta tgcgcgtccg tacctcggca tccggtcgct ctacgtatcc ggcgagctgg
     8101 cggaagcgct ggatttgcct gccgaaggcg ggttgctcgt tatcagtgcg gagcggagct
     8161 ctccggccga atccgccggc ctgcgcggcg cgcgccgccg ggtcgtcgtc ggcaactatg
     8221 agatccccgt gggcggcgac ctcatcatgg ccgttgacgg gcaggcggtg gacggcaaca
     8281 cgaccctcga gcgggccaag aaccgcaaac gcgttggcga ggctctcaat ctcacgattt
     8341 accgtgacgg gcgcacgatg aaagtcaaag ttaccctcgg cgaagccccc gaacagcgcc
     8401 tctaaccacc atttcgagaa aacggcaaaa ttgatggcgg ttcccggcat tcatcaaggc
     8461 gcggtatagt tgtcgattgc gccgtggtac ttgtccaacg ggtctcgaag gtttatcctc
     8521 tctaccgtcg tccctccgac cgcatcctcg aactctttcc cttccgacgt acccctcttc
     8581 attcggattt ctgggcgctc cgggacatca gtttttctgt tgagcgcggt gagtttctcg
     8641 gcatcgtcgg gccgaacggc tcgggcaaga gcacactcct tcaaatcctc agcggtattt
     8701 tgcagccgac ctccggacga gtggtcaccg agggccggat cgcggcgctg ctcgaactgg
     8761 gcgcggggtt caatcccgaa ttcacggggc gcgagaacgt ctacatcaac ggcgaaatca
     8821 tggggctttc gcggcgcgag atcgagcggg cgttcccgaa aatcgaggcg tttgcggaga
     8881 tcggcgagtt catccacagg ccggtgaagg aatactcgag cgggatgtac gtgcggctgg
     8941 cgttttcgac ggccattcac gtggatcccg agatcttgat cgtcgatgaa gcgctggcag
     9001 tgggtgacgc aatcttcgcg aaccggtgca tccagaaatt cgaagagctg aaggatcgca
     9061 aagtaacggt actgttcgtg tcacacgatc tgggccttgt gaaacggctg tcgaaccggg
     9121 cgatcctgat ggtgcaaggg caggtggttg cggaggggaa tccgagcgac gtcgtcaatc
     9181 ggtatgtcgg actcgtgcat aagcgtgggc ggctggaaga ggcacgcgaa gggagggcgc
     9241 tgacggccag ctaccgccac ggagatggcg cgagcagagt cgtggaagtt gaactgttga
     9301 atgaggcggg cgagcctgtg tcgacgatcc agagcggcga gcaggtcact gttcgcgcgc
     9361 gggcgaagtt tctgcggccg gtagaggagc caatcgtcgg gattctgatt cgaaaccggc
     9421 tggggacgga cgtcttcggg acgaacacga agctggagaa aagaaagctc ggcatgttcg
     9481 cgccgggcga tctgttgacg gcggagttcc ggtttaattg cctgctgtcg cagcaggaat
     9541 atacactgac tgttgccacg caatactggg acgggtccag ccaggactgg ctcgatgacg
     9601 tgctgggctt ccgggtagtg gacgagaagg cggcggctgg attgctcagt ctgaagacgg
     9661 aaatcgaatg gcggaaagaa tagcgccggc agtggacgga caggcagcac gttttttcgg
     9721 acaccctcgc gggctggcta cgcttttcct gacggaattt tgggagcgct tcagctatta
     9781 cggcacgcgg gcgctgctga tcctattcat gacggcgccg gcggccacgg gcgggctcgg
     9841 cttcgacgtg gggaaggcgg gtgcgattta cggactctac acgtcggcgt gttacctgat
     9901 gagcctgccg ggcgggtggc tggcggacca gtttttcggg cagcggcgcg cggtgctgta
     9961 tggcgggtcg gtgatggcgc tggggaacct actgctcgcg gctccgaacg tggcgttgtt
    10021 ttatccgggg ctggcgctgg tggcgctagg tacgggtctg ctgaagccga acgtgagcgt
    10081 gatggtggga cagctttacg cggacagcga tgcgcggcgg gacgcgggtt tctcgatctt
    10141 ctacatggga atcaatctcg gcgcgttcgt gtcgccgctg gcgtgcgggt acctgggaca
    10201 gagagtggac tggcacctcg gcttcgcgct ggcagggttc ggaatgatcg ccgggctcat
    10261 tcagtatgtc gccggcggga agcacctggg aaaggcgggg ctgaagccgg cgcccgtgaa
    10321 cgccgaagag tcgaagcggc aactccggaa gctgagaatc gtgctactgg cggcgggcgt
    10381 catgttcggc ggagcggtgg cgtttcaggc ggcgggcgtg atgcgcatca ccgcgcaggc
    10441 gctttccgat gccctgggtt ggatcctggt gttgatcgtg gcggcgtttt tcgtatgggc
    10501 cctgtttctg ggcgactggt cggcggtgga gcggaagcgg ctggtggttg tcggcgtgct
    10561 gttcctggcg tcgtcgttgt tctggtgcat gtttgagcag gcaggctcga cgttgaacct
    10621 gtttgccgag cggaacaccg acaagacggt cctgagtttc gctgtgccgg cgagctggct
    10681 acaagcactg aacgcgctgt tcatcatcct tctggcgccg gtgtttgcct ggatctggat
    10741 taagcttgga cggcgggagc cgtcaagccc gacgaaattc gtgcttgggc tggtggctgg
    10801 cggcttgggg tacgcggtgc tggtggtggg tgcgtcggtg gctgcgcaag gagtgcaagt
    10861 gagcctaatg tggcttgtgg tggtctacct gttccacacg gttggtgaac tttgcctgag
    10921 tccggtgggg ctgagcgcga tgacgaaact ggctccggcg aagatatcgg gactgctgat
    10981 gggtgtgtgg ttcctgtcga tttcggtggg caactatatg ggcggacggc tggcgtcgct
    11041 atacgaatcg ctgccgctga cggaactgtt tggcgcggtg gcggcgttcg ggataggcgc
    11101 ggggttggtg ctggcggttt ttgtcaagcc ggtgaagcgg ctgatgggcg gcgtcaagta
    11161 gcaggattca ttcagatgaa actcgacgaa attcaagcgg cgttgcggga gcaggggctc
    11221 gacgggtggc tgttcttcga tcatcaccag cgcgatccac tggcgtaccg ggtgctggag
    11281 tttgcgcccg aaggcccggt gacgcggcga tggtactacc tgattccagc agacggtgaa
    11341 ccgaggaagc tcgtgcacgc gatcgagtcg ttcgtcctgg acgcgctgcc gggcgccagg
    11401 ctagtgtatt cgggctgggc acggcagacg gagggactgc tggaactgct cgcgggttgc
    11461 cggagagtcg cgatgcaata ctcgccgcaa tgcgcgattc cgtacgtctc gatggtggac
    11521 gcaggaacgg tggaactcgt gctggcgacg ggcgtggaaa tcacgagctc ggcagacctg
    11581 atccagtatt tcgaagcgcg gtgggatgag gagaagctgg agagccatct cgaggcggga
    11641 cggcgggtgg atgagatccg cggaaacgcg ttccgcctga tcggcgagaa gctgcgagcg
    11701 ggagagcgga tccgtgagtt cgacgtgaag caattcattc tggattcgtt cgccgcggcc
    11761 ggtatggtca cggaccacgg gccgatcgtt ggggtaaacg cgaatgcggc gaatccgcac
    11821 tacgagccgt cagcggatgc gagcgaggaa attcggcgag gcgacctggt actgatcgac
    11881 atgtgggcga aactggcgaa gccgcggagc gtctattacg acatcacgtg gacaggcttc
    11941 tgcggaacgg agccgcgggc tgaaatcgaa agggtcttcg ggattgtacg cgaagcgcgc
    12001 gatcgggcgg tgcggcgcgt gcggaaggca gtggaagaag gcgagccgtt acgggggttc
    12061 gaggtagacg atgcggcgcg ggaatacatt cgagagcagg gtttcgccga ccaatttgtg
    12121 caccgcacgg gccactcgat cggcgaggac gtccacggag caggcgcgaa catggacaac
    12181 ctcgagacgc acgacgagcg gagggtgatt gctggaacgt gcttttcggt cgagcccggc
    12241 atttacctgc gggacttcgg gatacggtcc gaagtgaacg tgtacgtcgg tgagcgaagc
    12301 gcgcgtgtga ccggagaagc acaggaacgg ctggtgctca tctgatgcga cgcgttgcgt
    12361 ttctcgctct tgcgatggct ggcgcgtgtg tcgccgtcga ttcccatcca aaggccttcc
    12421 cgctgtcggc gcaggacggc gtggcgctga tcgagtgggt cagcgcgtcg acgttccgat
    12481 tctgccgcgc gtgggagact gcttcgtgca agccgggcgt gaatcgcgag gtggaagtcg
    12541 agcgtgagga cgcgggagcg cgactccgat tcaggaccaa gtacctgcac gtggatgtcg
    12601 acaaagccac actgcgggtt tcggcgcgta cgccggaagg tccggagcta ttggaggacc
    12661 tgaccgaggc tcgccgtgcg gagggcgccg tgacgatcga gagatcggcg ccggtagggg
    12721 agcggttcta cggcctcggg gcccatgcat acgagtcggc cgatcttcgt ggaacagcga
    12781 tggacactac caagccgttt ctcatatcga gccgcgggta cggtctgcac cacgtatcgc
    12841 ccggcaggta taccttcgat ctggggcgga agcacagcga ccgataccgg atcacggtgc
    12901 gcggggcgag gaaaatcgac tactacttcc acttcggtcc ttcgccgaaa gagatcctcg
    12961 aagagcataa ggaagtcgcg gggacgccgg acatcctaaa agagtggcag ctcggaatca
    13021 tcgcgcagac gcagttgccg gcctatgcaa cgccgctgcc ggcgacgctc gatgcgtcgt
    13081 gggaatcact gtgcgactgc gtccggatga tgtcgcatgc gtcgctttcg ggaatcccgc
    13141 tgccggcgtt cgacatcgga ctgtatcggt caaactcgct tgcgctgtac cggcgggcgc
    13201 tgcaactggc gcaggtggcg ccggtggtat tcagctcgtc ggccgcgtcg gcggacgcgg
    13261 aaatgcttgt gatggatgag agcgccgcgc aggcgcgccg ccggtggctt ccgttctttc
    13321 tcacgtacgc ggatgaggcg aggctccgag ggctcccgat gctacattcg ctgccgctcc
    13381 agtttccgcg cgatcacgag gcaagcaaga gagcggacga gtttctgatg ggtgatgaga
    13441 tgctggcagc gccagtgtgc accgaggacg gccggagaag cgtctacctg ccgatgggtg
    13501 tgtggacgaa ttggcggaca aacaaggttt acccgggacg ccagacgatt gaaatagagg
    13561 cggcggagga cgaactgccg ctgttcgtga agaatgggac gatcgtgcca gcgggaccga
    13621 ggtcagggtc ggcgccgatg gaactgcact actttccaaa actggcgtca gagttcttcc
    13681 tgttcgagcc ggaggtttcg gactatacac aggcgcacgc gaatccggcg cttgacttgt
    13741 ggcggctgga gatagcgccc aaggtggacc ggacgtacga atgggtgctg cacaatctgc
    13801 ctgcacccac gcgagtgtgg gagggcgaaa cggagtacca aagggtccag gatcgggggt
    13861 tgctcaagcc gggctgctgg tattttgacg aggtgacctc aaacattcat gtcacggtgc
    13921 ggacgctcaa gggagcgacg cgcatcgttc acatgggaca gtaggttggc gtgataggaa
    13981 cagcgggccg atgctcagat cttgagaaag atcctccgct gatacagcca gtagcagaga
    14041 taccacatca cgcccaaggt ggcgaggttc tgagggatct cgccgagtct gccgaggtac
    14101 cagaaattcc cggtgaatac accgaggccc tggtacagcc acccgtgcag gatctgagaa
    14161 aacgaataaa tgaagattga gttcattccc agcacgatga acggataagc ccatctcctg
    14221 taaccgagga cttcgaccag ccagtaaaac accatcagca taaggatgac ccagccagcg
    14281 gagtaaaacg tgaacgacgc cgagttgatc aagcggtagg ggatcaccgc agcggcggtc
    14341 ttccatccga gccgcgtcat ttcgagagcg gcgagcgcag ggagcagttc gaccggctgc
    14401 ggcccaggac ccggttaacc tttgtaagta tttacaggac aacggtctgc tggcagaatg
    14461 gggctgtgaa gtgccatctg ccgacatgcc gtgaagaggt tcggccggag cagcagcgcg
    14521 tctgtgtcaa cggcaggcag gactatcact ggcactgttt cttcctccag ataagccggt
    14581 tgatctccca cggcggagcc aaactacgcg accttaccga tcaagttacc gacctgaatg
    14641 gagatcgaag tcaaaacgtg caggaagtat aggcccttca agacgacggg ggatgggcac
    14701 agccggccga tactcagacc ttgaggagta tcttcctccg cccggcgaaa taagacatga
    14761 acgataccgt tgcgccccgc tgaaacgcag tgctacgctc agcccaaggg ccaattccgc
    14821 tcggaggagc accatgagag ccatctcggc gtttgccgtt tgcagcgtgt tgttcactgg
    14881 attgacggtt cagggagcaa cacacctcaa ggttggggac accgcgcccg actttacgct
    14941 tccttcttcg accggcaagc cggtcagcct gtcggaattt cgcggcaaga aaggggtcgt
    15001 gctggcgttc tttccggctg cgttcactgg cggttgaaca cgggaaatta cggcgtacca
    15061 gtctggtatc gctggatttg aaggaatgga cgtgcaggta ttgggaatca gcaccgacgg
    15121 ccagttcact ctgaagcact gggccgacga gcaccttaag acgacgtttc ctcttctgag
    15181 cgactttctg cgcaaaacgt cggagagcta cggcgtgctg aacgcggaaa gtgggatggc
    15241 gaaccgcacc acgttcgtga tcgacacgga gggccgtatc caacacatcg aagaaggcaa
    15301 gagtgcgatc gacccaagtg gggcgatgac ggcatgcagc cgggtccgaa agaagtaggc
    15361 gccgcgctac cccactgaaa ctgccagcct gtcgcctttg cccagccgga gcggtttctt
    15421 cgggcggatg attgcctcgc cgcccgcggc ttcgacggtc gcgccggcga gcgatgatcc
    15481 ggacggcttg ccagggaagg gaagtcggag ctgcgccagt tccagcgcac caccctcgac
    15541 ggtgatctcg gcgtccagtt tctgtttcgt gcgcttctgg gaatagttgc cccaggcagt
    15601 cccggtcgaa tacagagagc ggaagttgtc ctgactcaca agcgggctga accgcagggc
    15661 cttctcggaa gacgaccact ggaatccgga cagcgccagg accaacgacc aactcgacat
    15721 cgcccgcgca tagtggtggc cgcactcgat ctcgttccag gggttgcgcc ggacgccgtc
    15781 gtaacggttt cgtacggcat ccacgatcgc gagtccctcc ttgaacatcc cttcgtaaaa
    15841 caggtgtgcg gcgacctgat actcaatccc ggtccatacc tcatcggagt acacgaatgg
    15901 aagagctggg cgatggccac gtggccaact gcacaacagc agacctgcct cgtcgttcaa
    15961 ggcatagagg cgttgcgcgt tcgggaagtc ggagaagtcc tctcggaagt tgtggcggaa
    16021 gatagcgcgc agggtggact ggatatgctc gtggggcagc agcttgccga ggccgaccac
    16081 ttccgcgaac cactggccga gcaactggtc cgacaggcag ccctccccgt actgatacct
    16141 ggaggctttt tcggcacgct cgtcgatctt ctgaacataa tactcgccgt tccatagcag
    16201 tttgtccaac cgggcagcgc cttcttccat cagcttccgg taacgctcag cggcggcgtt
    16261 gtcgccgaga tgcactgcca tcttcgacgc ggcggcaagc gcaccgaggt agaaggtgcc
    16321 catcatcgta ttgggcccat agaattcgat gtcgtaggtg ttatgctgct cgccctccat
    16381 tacgcc
//
LOCUS       DATIFQ010000069         4641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_789279, whole genome shotgun sequence.
ACCESSION   DATIFQ010000069 DATIFQ010000000
VERSION     DATIFQ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789279"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VLE22_03010"
     CDS             <1..431
                     /locus_tag="VLE22_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13400.1"
                     /translation="QGDEVRLEQIPEPVEKPSLVRELVQAVLNRKVVFMGNGLNIGNA
                     PGNVSDLESLIAAQMGQTTIIGNALNLGSLAPPARPSSSVSAASLAKLKQLFDAKKYA
                     DALSTVNGLRAKDPQSAELKAWKARILEGMAPSMAPPKPR"
     gene            complement(494..1852)
                     /locus_tag="VLE22_03015"
     CDS             complement(494..1852)
                     /locus_tag="VLE22_03015"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HSB13401.1"
                     /translation="MRLRNAGLLWLCTAGLLCAQFPGPYPDPFPREPPRSRGDRGPGG
                     RLPAPANVLQQFTGKIDKIENDSITIRVPDSRLITFTCSKITKYFEHDKEIQRAALKP
                     GDEVEVEARSDNEGYFHAVNVKLEKAVEPLATETAPEEVPAQKHEAIVIGSAAAKDPD
                     VPILRRGKPAPRPHPAEDEPENGPAAAASPAAPPATAASAAWSESDSFLEKAREVANE
                     FSETLPNYICNQLTTRYESEGRPVSWRALDVVSAAVAYEDGVESYHDIRINNKPVNKA
                     MDQIPGSRSYGEFGNTLRGVFSPASAADFHLRRDSTASGRPATIYDFEVEQPNSHWQT
                     TVGGQSVLPAYKGSVWIDKITSRVLRIEMQALNVPETFPLDTIEWVVDYSFVRIGDTE
                     FLLPAHAENLSCWRGTLRCGRNALDFRNYRKFTSESTITTSDSTVRFEAEEHPEPATA
                     NK"
     gene            2156..2758
                     /locus_tag="VLE22_03020"
     CDS             2156..2758
                     /locus_tag="VLE22_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13402.1"
                     /translation="MKLAIALSFLFSTLAAQAPLDERGKSGVVEFFVVDSNGVPVKGD
                     ITFSVRQLRDGKTAREVKVGAKASLAYGTYKLTVQGSPAYPVEKIIKVKEAYQPVLIG
                     LFVAPIELPWAGNVVRGKLPRASAERGCLSVRLVSPVAENEYADARALASGEFGFENI
                     KPGRYLLVTFGERGICEVSPVSVFDKRTQELFIDVGSLRP"
     gene            2776..3525
                     /locus_tag="VLE22_03025"
     CDS             2776..3525
                     /locus_tag="VLE22_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13403.1"
                     /translation="MHELLHKNMIGGVFTHGQLRSTVDVVGTVTTVGRNAISDQIGKI
                     CCARIIRFVMVLCVASACLQGEEALVVSPQIFEIKYCREAGGGLSMRTVLQLTVRNPG
                     TTSIILARFTRLSAFSLFADQKDIELNRPETGEISSPWRMFDASKLNKAEPAPELFEV
                     IKPHASCELGMQEIRIPLKPGRPGLRLMGKDHYLRLAFNPWPESRRSGEELRRAWASY
                     GRLQIESLVSLPIKLHIDADPAAKACIERID"
     gene            3678..4028
                     /locus_tag="VLE22_03030"
     CDS             3678..4028
                     /locus_tag="VLE22_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13404.1"
                     /translation="MRNKRWLLAVVALGLPLIAQDPVKVDPKHYKVEFENSDVRVLRI
                     TYGPGEKSVMHEHPNAVAVFLMDQMTKFTFPDGKTEDLRTKAGETRWTAAGKHLPENT
                     GSKPFELVLVELKK"
     gene            4147..>4641
                     /locus_tag="VLE22_03035"
     CDS             4147..>4641
                     /locus_tag="VLE22_03035"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HSB13405.1"
                     /translation="MSHPILVTGAAGRAGGVGRTITELLLKQGKAVRAMVRNEDERAQ
                     ALRDKGAEVVVGNLLDLDSMHKVIAGCETMYFGMSVSDDYLAATVNTAAVAKHHGVKA
                     FINMSQMTLSQMSITQTTASPQHKLHWLAEQALNWSGLPVVHVRPTVLLEGFFLIFTS
                     DSVRE"
BASE COUNT          953 a         1290 c         1468 g          930 t
ORIGIN      
        1 tgcaggggga cgaagtccgc ctggaacaga tcccggagcc ggtggaaaaa ccctctctcg
       61 tgcgcgagct tgtgcaggcc gtcctaaacc gcaaagtggt cttcatgggc aacggcctga
      121 acatcggcaa cgcccctgga aacgtctcgg acctcgagtc gctcatcgcg gcgcagatgg
      181 ggcaaaccac aatcatcggc aacgcgctca atctcggatc gctggccccg ccggctcgcc
      241 cgtcctcttc tgtgtctgcc gcctcgctcg caaagctcaa gcagcttttc gacgccaaga
      301 agtacgccga cgccctctcc acggtgaacg gcctgcgagc caaagacccg caaagcgccg
      361 aactcaaagc ctggaaagcc cgcatcctcg agggcatggc cccgagcatg gcgccgccga
      421 agccgcgttg acagtggccg gcgacggtcc ccggtaccac aaaggggagg gcgcagtacg
      481 tgcaggccca catctatttg ttcgcagtgg ccggttcggg gtgctcctcc gcttcaaagc
      541 gaacggtgga gtcggaggtg gtgatcgtcg actcgctcgt gaacttgcgg taattgcgga
      601 aatccagggc attgcggcca cagcggagcg tgcctcgcca gcaggagaga ttctcggcgt
      661 gcgccggcag taagaactcc gtgtcaccga tgcgcacgaa ggagtagtcc actacccact
      721 cgatcgtatc cagcgggaac gtttcgggga cgttaagcgc ctgcatctcg attcgcagga
      781 cacgcgaggt tattttatcg atccacacgg aacccttata ggcgggtagc acggactggc
      841 cgcccacggt agtttgccag tgcgaattgg gctgttccac ctcgaagtcg tagattgtgg
      901 caggccggcc ggaggcggtg gaatcgcggc gaaggtggaa atcggcggcg ctggcggggg
      961 agaagacgcc gcgcagggtg ttgccgaatt caccgtacga ccgggaaccg gggatctggt
     1021 ccatggcctt gtttaccggt ttgttgttga tcctgatgtc gtggtagctc tcgacgccgt
     1081 cttcgtacgc caccgcagcg ctcaccacgt ccagcgcccg ccagcttacg ggacggccct
     1141 cgctctcata gcgtgtggtg agttggttgc aaatatagtt ggggagcgtt tcggagaact
     1201 cgttggccac ctcgcgggct ttttcgagga aggaatccga ttcgctccac gcggccgatg
     1261 cggcggtggc cgggggcgcg gcgggggaag ccgcagccgc cggcccgttc tcgggctcat
     1321 cctcggcggg gtggggccgc ggcgcgggct tgccgcggcg cagtattggc acgtccggat
     1381 ctttcgccgc agcggagccg atcacgatgg cctcgtgttt ctgggccggc acttcttccg
     1441 gggcggtctc cgtggccagc ggttccacag ctttctccaa cttcacattc acagcatgga
     1501 agtagccttc gttgtccgaa cgggcttcaa cttccacctc gtcgccgggc ttcagagctg
     1561 cgcgctggat ctccttgtcg tgctcgaaat acttggtgat tttggaacac gtgaaggtga
     1621 tcaagcgcga gtcgggcacg cggatagtga tggagtcgtt ttcgatcttg tcgatcttgc
     1681 cggtgaactg ctggaggacg ttcgccggcg caggcagtct gccgccgggg cccctgtctc
     1741 cgcgcgaacg aggaggctct cgtggaaagg gatccgggta tggtccggga aactgggcgc
     1801 agagcaggcc agccgtacac agccataaca ggccggcgtt tcggagccgc aaacaagcac
     1861 ctcctatctt ctggacgtcc gaggcagcgc cggagtttac gggattttgg aggtgagtcg
     1921 cggcatttga ggaactgggc cgacgagggc gtcggccgtg gcggcgacat tggacccaga
     1981 ccgggttttc cgtgtatcca ggaagcagta aggctgcttc atgctgggtg atatgatgcg
     2041 aggcgccggc ccccacagac gcgggcaagg catgcaataa cggcacgggc agcggaggtt
     2101 ggggttacgt agcatgtcgc cgataccccg atacttcgtt ttgaggacaa cccgagtgaa
     2161 acttgcaatc gcattatcgt tcctgttttc gactctggca gcacaagcgc ccctggatga
     2221 gaggggcaag agcggggtcg tcgagttttt cgtcgttgac tcgaatggcg taccagtgaa
     2281 gggggacatt accttctcgg tgcgacaact ccgggatggg aaaaccgcgc gcgaggtcaa
     2341 agtgggcgcg aaggcaagtc tggcctacgg cacctataaa ctgacggtcc agggctctcc
     2401 cgcgtatcca gtcgagaaga tcattaaagt gaaagaggct taccaacccg ttctgatcgg
     2461 tctatttgta gctccgatcg aactaccgtg ggccggcaat gtcgttcgcg gaaaacttcc
     2521 gcgggcaagt gccgagcgcg gttgtctatc ggttcgtctg gtttccccag tggcggaaaa
     2581 tgaatacgca gatgcgaggg cgctcgcgtc gggtgaattt ggatttgaaa acataaaacc
     2641 cggcaggtac ctactggtca cattcggtga gcgcggcatc tgtgaagtgt caccagtaag
     2701 cgtgtttgac aagcgcacgc aggaactgtt catcgacgta ggatctttgc gaccttagct
     2761 gctgtgaaaa ttctcatgca cgagctcctc cacaagaaca tgatcggcgg cgtattcacg
     2821 cacggccagc ttcgaagcac agtggacgtt gtagggacag tcacgacggt gggacgaaac
     2881 gcaattagcg accagatagg caagatatgt tgtgcgcgga tcatacgctt tgtgatggtg
     2941 ctgtgtgtcg catcagcgtg tctgcaaggc gaggaggcgc tcgtagtaag cccgcaaatc
     3001 ttcgagatca aatactgtcg cgaggcagga ggtggactca gtatgaggac cgtgcttcag
     3061 ctcactgtcc ggaatcctgg aaccacaagc attattctcg cccggttcac acggctatct
     3121 gcattttcac tttttgcaga ccaaaaggac atcgagttga atcggccgga gacgggcgag
     3181 atctcttccc cttggcggat gttcgacgcg tctaagctga ataaggccga accggcaccg
     3241 gaactatttg aagtgattaa acctcatgcc agctgcgaac tggggatgca ggagatcaga
     3301 attccgctga agccggggcg gcccggtcta cgattgatgg gcaaagacca ttaccttcga
     3361 ttggctttca acccttggcc cgagtcgcgc cggtctggcg aagaacttcg cagagcatgg
     3421 gcctcgtacg gacgccttca gatcgagagc cttgtatcgc tgccgatcaa gctgcatatc
     3481 gatgcggacc cagcggcgaa ggcgtgcatc gaacgaatcg attaaagccg tcgccttaag
     3541 agtcacagcg ccaaggggtc gattcaagta gtccgattgc gtctccaccg attcgtcctc
     3601 ggggttgagc tatactgctt ccaacgtcca cagcaaactt gaagttgctg cggatattca
     3661 ctcagaggag aatttgcatg cgaaacaaac gttggcttct ggctgtggtg gcgctgggtc
     3721 tgccgctgat cgcccaggat cctgtcaagg tcgatccgaa acactacaag gtcgaatttg
     3781 agaattccga tgtgcgggtg ttgcgcatca cttatggacc cggggagaaa tccgtgatgc
     3841 atgagcatcc caacgcggtg gccgtcttcc tgatggacca gatgaccaag ttcacttttc
     3901 cggacggcaa gacggaggac ctccgtacca aggcaggcga aacgcggtgg acggcggcag
     3961 ggaaacacct tcccgagaat accggctcga agccttttga actggtgctg gtcgaactga
     4021 agaagtaagt gacctgtggg ggacgagcgg cggcccctga ggccggtttc tgcctcacaa
     4081 taccggccga cgctcgcatt caacaaatct gacttcgatt caggtgaacg cacagaagga
     4141 aagaacatgt cacatcccat tctggttacc ggcgcggcgg gccgcgccgg cggggtcggg
     4201 cggacgatca ccgaactgct gttgaagcaa ggcaaagcgg tacgtgcgat ggtgcgaaac
     4261 gaagacgaac gcgcgcaggc gttgcgtgac aagggtgcgg aggtcgtggt tggaaatctg
     4321 ctcgatctcg attcgatgca taaggtgatt gccggctgcg agacgatgta cttcggcatg
     4381 tcggtttcag acgactatct ggccgcgacg gtcaatacgg cggcagtagc gaagcatcac
     4441 ggcgtgaagg cgttcatcaa tatgtcgcag atgacgctgt cgcagatgag cattacccaa
     4501 accaccgcga gcccgcagca caagctgcac tggctcgccg agcaggcgct gaactggtcc
     4561 ggtctgccgg tcgtgcacgt gcggcccaca gtgcttctcg agggcttctt cctgattttc
     4621 acttccgatt cggtcaggga a
//
LOCUS       DATIFQ010000070         4231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1172574, whole genome shotgun sequence.
ACCESSION   DATIFQ010000070 DATIFQ010000000
VERSION     DATIFQ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172574"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..540
                     /locus_tag="VLE22_03040"
     CDS             229..540
                     /locus_tag="VLE22_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13406.1"
                     /translation="MAKSAKGLFDLECPCCHAALKIDPETRAVISFKATEKPRSVEDI
                     DAAYNNWKGEADRREAAFRKSVEAEKKSKDVLSKKFDELLKQAKETPDLKPPTRDFDL
                     D"
     gene            complement(537..2429)
                     /locus_tag="VLE22_03045"
     CDS             complement(537..2429)
                     /locus_tag="VLE22_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13407.1"
                     /translation="MSTARLLLLSLPACAFFAAAQTTPQQMRAMLEPPLQTPDVVAFQ
                     LRHYLLKKIPKLPPASSAAQWTSEAKRLRESVLKDVVYHGWPREWVEAPLKVEDLGLM
                     PSGPGYRMRKLRYEIVPGMLSSAILYEPEKMNGKAPAILNVNGHVGPPGKSVEYKQKR
                     CINQAKQGILSLNLEWLSFGELSHPENVHWFAAHLDLTGANAAGLFYLAMRKGLDFLY
                     DHPNTDRSRIGVTGLSGGGWQTIVLSSLDERVAVAVPVAGYSSLRSRIERPGDTGDIE
                     QNATDMLTLADYPHLTAMRAPRPTLLVYNAEDDCCFRGPLVKPFIYDEVRPFFRLFGK
                     EAALEWHENTDPSDHNYQLDNRQQGYRFFATHFGMPPAGSEIPVDSEIKSYDELVVGL
                     PKDNLTILGVAKKLASMREQAAPATAGKAELASTVRIRETKVAEVLAVRSSKAKGLET
                     RSYRFDFENGLSAAGVWLKAIAEPAGAPATIVLNDKGKKSAGEVASDRVNRGEQVLAA
                     DLVFTGDGAPEDTHLYAEMLAATGDRALGLEAAQLAALASWLGSQTGAGKVRLEAAGI
                     RSQVTALMTATLYPDLFSDVVVREGMESLRHLLDKPVEYQAAPDLFCLGLYPEFDVAR
                     LEKMRR"
     gene            complement(2509..3936)
                     /locus_tag="VLE22_03050"
     CDS             complement(2509..3936)
                     /locus_tag="VLE22_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13408.1"
                     /translation="MKSTLLSGATLLLLAGSVPAAAGEIRLETRLSGGAIGGLEPSGH
                     AEFRMRAGETRFSAEVEDVNLPADTVLRVSVRGSEVGTITIGPAPVRGGDLNLDSRTT
                     GPVMEMRSGDTVTVAGPGGSILAGVLHERAAGERRLRGDDLADVNRGDDALRCQAAGE
                     AVMLRSALSGGPIGGVEPLGSAQFRAGGAGGEARFSVEVEHLNLPAGTVLDVFVNGNR
                     LGSITLAGERFRGGELELETHGGNGTLAIKGGDTVTVSGPSGLVLSGVLQPFSFCEPS
                     APAVPQTQGHDGGDDDAPSLETRLAGGALGGLEPSGHAEFQNRPERNEREFKVEVEDV
                     NLPAGTMLDVFVGGQKAGSIRVGPTPERGGELELKSEHGDIVPSVNDGDLIVVAVQGT
                     NAPILSGVVRMRSASTEARDDHGMQSPGTDDGPDDHKQGSGGSSSGSGSSSGSGSGDN
                     SGSGSGSGHDDDHSDDHGGDSSGKH"
BASE COUNT          762 a         1472 c         1214 g          783 t
ORIGIN      
        1 ccgcgatgcc aaaacgaggt tacagatatg agggaatggc aggctatgtc ttcggcaggc
       61 cggctgacgc cggccaagga aaaagaaaat gcttgacacc gaccggcctt ctcatggaag
      121 ccgcgaccgc aagggagcgg tggttgaggt atttcattac gttcgcccat ctccacctct
      181 gtgtcgcgca ccctctgctt cccgcctcaa agttgctagg atgaccctat ggccaagtcc
      241 gcgaagggtt tgtttgacct ggaatgcccg tgttgccacg cggcgctgaa aatcgacccg
      301 gaaacgcggg ccgtgatcag cttcaaagcg accgaaaagc cgcgctcggt cgaggatatt
      361 gacgccgctt acaacaactg gaagggtgag gccgaccggc gcgaggcggc cttccggaag
      421 tctgtcgaag ccgagaagaa gtccaaggac gtcctgtcca agaaattcga tgagctcctc
      481 aagcaggcca aggaaactcc ggacctcaag ccacccaccc gcgacttcga cctcgattag
      541 cggcgcatct tctcaagccg ggccacgtca aactccgggt acagtcccag gcagaagagg
      601 tccggcgccg cctggtactc gaccggtttg tccagcagat ggcgaaggct ctccatgccc
      661 tcccgcacca ccacatccga gaacagatcc ggataaagcg tcgcggtcat cagtgccgtc
      721 acttgcgagc ggattcccgc cgcctccagc cgcaccttac ccgccccggt ttgagatccg
      781 agccaggacg ccagcgccgc cagttgcgcc gcttcgagcc ccagtgcgcg gtcaccggtc
      841 gccgcgagca tctccgcgta gagatgcgta tcctccggcg ctccatcgcc tgtgaacacg
      901 aggtcggccg ccagcacctg ctcgccccgg ttcacgcggt cgctcgccac ctcgcccgcc
      961 gacttctttc ccttgtcgtt gagcactatg gtcgccggag cgcccgcggg ctccgcaatc
     1021 gctttcagcc agacacccgc cgcgctgagc ccattctcga aatcgaagcg gtacgatcgc
     1081 gtctcaagcc ccttcgcctt gctgctccgc accgccagga cctccgcaac cttcgtctcg
     1141 cgaatccgca ctgtgctggc aagttcggcc ttgcccgccg tggctggcgc cgcttgttcc
     1201 cgcatcgacg ccagcttctt cgccacgccg aggatcgtca agttgtcctt cggcagaccc
     1261 accaccagtt cgtcataact cttgatctca ctgtccaccg ggatctcgga gccggccggc
     1321 ggcattccga aatgcgttgc gaagaaccgg tatccctgct gccggttgtc gagttggtag
     1381 ttatgatcgg acggatcggt attttcgtgc cactcaagag ccgcctcctt gccgaaaagc
     1441 cggaagaacg gcctgacttc gtcatagatg aacggcttca ccagcggacc gcggaagcag
     1501 cagtcgtcct cggcgttgta gaccagcaag gtcggccgtg gagctcgcat cgccgtcagg
     1561 tgcggatagt ccgccagcgt cagcatgtcg gtggcgttct gctcgatgtc gccggtgtcc
     1621 ccgggccgtt caatccgaga tcgcagcgac gagtaccccg ccaccggcac ggccaccgcc
     1681 acccgctcgt caagagagct aagcacgatc gtctgccagc ccccgcccga cagtccggtc
     1741 accccgatgc ggctacggtc cgtgttcgga tggtcgtaca gaaagtcgag ccccttccgc
     1801 atcgccaggt agaacaatcc ggccgcattg gcgcccgtca gatcgagatg cgccgcgaac
     1861 cagtgtacgt tctccggatg cgatagctcg ccgaacgaaa gccactcgag attcaggctc
     1921 aggatgccct gcttcgcctg gttgatgcac cgcttctgct tgtactccac cgacttgccc
     1981 ggcgggccca cgtgtccgtt cacgttcaga atcgccgggg ccttgccgtt catcttctcc
     2041 ggctcgtaca ggatcgccga tgacaacatg ccaggcacga tctcgtatcg caatttccgc
     2101 atccggtagc ccgggcccga cggcatcagg ccgaggtcct ccaccttcaa cggcgcctcc
     2161 acccactccc tcggccagcc gtgatacacg acgtccttca ggacgctctc ccggagacgc
     2221 ttcgcctcgg acgtccactg tgcagccgac gacgccggcg gaagtttcgg gatcttcttc
     2281 agcaggtagt gccggagctg gaacgccacg acatctggcg tctgaagcgg aggctccagc
     2341 atcgcccgca tctgctgggg cgtggtctgg gcggcggcga agaatgcgca ggcaggaaga
     2401 gagagaagaa gcaggcgggc tgtggacata cagagatttc accacaggga cgcggagaac
     2461 gcagcagttc tccgcgtccc ctcggtgaaa tcgggcgact cggcagcgtc agtgcttgcc
     2521 gctgctgtcc cctccgtggt cgtccgaatg gtcgtcgtcg tgaccagatc cggagccgct
     2581 gccggagttg tctccggacc cgcttccgga gctactgcca gagccgctgg agctaccgcc
     2641 ggagccttgc ttgtgatcgt ccggcccgtc gtccgttccc ggactctgca taccgtgatc
     2701 gtcccgggct tcggtggaag cggagcgcat acggaccacg ccggacagaa tcggcgcatt
     2761 cgtgccctgt accgcaacga cgatcaggtc gccgtcattc accgacggca caatgtcccc
     2821 gtgctccgat ttcagctcca gctcgccgcc ccgctccggt gtcggcccca ctcggatcga
     2881 tccggccttc tgcccgccaa cgaacacgtc gagcatcgtt cctgcgggaa ggttcacatc
     2941 ttccacttcc accttgaact cccgctcgtt tctctcgggc cgattctgaa actccgcgtg
     3001 cccggacggc tccaaccctc ccagggcgcc gcccgctaga cgcgtctcga gactcggcgc
     3061 gtcgtcgtca cctccatcgt ggccttgcgt ttgaggaacg gcaggggcag atggctcgca
     3121 gaaagagaac ggctgaagaa cgccgctgag cacaagtccg gaaggccccg aaaccgtgac
     3181 tgtatcgccg ccctttatcg ccagtgtccc gtttccacca tgagtttcaa gctccagctc
     3241 accaccgcgg aatcgctctc cggccagcgt gatcgaaccg agccggttgc cgttgacgaa
     3301 tacatcgagc acagttccgg caggcaggtt caggtgctcc acctccacgg aaaaccgcgc
     3361 ctctccaccc gctcctccgg cacggaactg cgcggacccg agaggctcga cccctccgat
     3421 cggcccgccg ctcagggccg accgcaacat caccgcttcg ccggcagcct ggcatctgag
     3481 tgcatcgtct cctcggttca catcggccag gtcgtcgccg cgcagcctcc tctcgcctgc
     3541 cgcccgctcg tggaggactc cggccaggat ggagccgcct ggccccgcga ccgtgaccgt
     3601 atcgccactc cgcatctcca tcaccgggcc ggtagtgcga gagtccaggt tcagatcgcc
     3661 gccacgcacc ggcgctggcc cgatcgtgat cgttccgact tccgatccgc gtactgaaac
     3721 ccggagtacg gtgtctgccg gaagattcac gtcctccacc tccgcggaga agcgcgtctc
     3781 gcccgctctc atgcggaact cggcgtgacc tgatggctcg agcccgccga tcgcgccgcc
     3841 gcttaaacgt gtctcaagtc tgatttcgcc agccgccgca ggtacgctcc cagccagcag
     3901 taggagcgtc gctccggaca gcagagtgct cttcaacttt cgccccttag tcgctccatt
     3961 agggaattca cttgtgtttc cagttttcat cttccatcca acctttcccc ggagagatcg
     4021 tcgctccgtg acgctattct gtggcgcgtg gacacacggc gtcgcaaagg cccagtaaaa
     4081 gaattgtgaa aacgtggatc taaaaggtgg gagtcttcgt ctcggagatg cgggaaaggc
     4141 ggacggtctc cgcacccccg agatcgaact acttctgtga agcgctcaac gcctggtcga
     4201 ggtcccagag gatgtcgtcg aggtcttcca g
//
LOCUS       DATIFQ010000071        12639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_215025, whole genome shotgun sequence.
ACCESSION   DATIFQ010000071 DATIFQ010000000
VERSION     DATIFQ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12639
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215025"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VLE22_03055"
     CDS             <1..1175
                     /locus_tag="VLE22_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HSB13409.1"
                     /translation="AARRTPPALAHPNDRMGRFAPMKQLWCVSTLTLFAALGSVAAES
                     DAQEAQDDKARQWSVGPVRFKGLIDGYYSLNLNHPESRQNQLRNFDVKANQFSLNMAE
                     LVLQHDPEPLGFRVDLGFGRAFDMIHATEPAPNVFRNILQAYVSYKPSKKSDFQVDFG
                     KFVTGAGAEVVETHLNWNYSRSLLFALAIPYYHFGARATVPLHHHFTAGAMIVNGWNN
                     VEDNNSGKTVGVTGTFHSPWFTWTNNYYVGPEWPGVDHGRRHLYDTTLLFNPSAKAAF
                     YVNFDYGAEKNPAGNSRWLGVAGAARLALSDRFAVTPRIEWFHDANGFATGTAQRLKE
                     FTLTGEYKVASGVLTRLEYRRDWSDQPFFERGSTPATFRNQATVLIGFVAFFGPER"
     gene            1292..2479
                     /locus_tag="VLE22_03060"
     CDS             1292..2479
                     /locus_tag="VLE22_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSB13410.1"
                     /translation="MKTNTVISVSAVFLVAIAFAFQKSVKLPPPFHSPSSNNPPRVIS
                     RPDGAQLRLPAGFDIDEYAGGFERPRFMLQGPSGEIVLSDSVEGGSVYVLLDKNNDFK
                     ADERNVLLRELDRPYGLAFWKDYLYVAETTSLKRYKYDRKTMTAGRGEEVVSMKDFGS
                     GHWTRTVLFDRKGEKMYLGIGSRSNVSPGEPERRAAISRFNPDGSGYELFASGTRNPI
                     GLRWYPGTDTLWAAVQERDALGDDLVPDYFTTIQHGGFYGWPFAYIGPNEDPRNKGQR
                     PDLVKKTIVPDVPLQSHVAVLDCLFYTGKQFPAEYRGGAFLAFHGSWNRSKRVGYSVT
                     FLPYKGSKPSGPVRDFLTGWMLAPDKREVWGRPVGLLELPDGSLLVSDDGGNKVWRIS
                     YKG"
     gene            2494..3108
                     /locus_tag="VLE22_03065"
     CDS             2494..3108
                     /locus_tag="VLE22_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSB13411.1"
                     /translation="MALAASVPAVPADRDAERRPFSHRLHLKLRLDCQTCHVSAARSS
                     RLEDNNLPPKEVCLRCHKQAVVKAPRTTAIARFDHSKHIKIGDIGKVIAAAIDQGTYL
                     SSAEGLKQKLTSGQPCKGCHRGLEESDEVTKAAFPTMADCLVCHSKIQPPDSCLFCHN
                     EGQKLKPDDHTPDYIDLHTGGKISLKKESCAVCHGKRFTCMGCH"
     gene            complement(3136..3501)
                     /locus_tag="VLE22_03070"
     CDS             complement(3136..3501)
                     /locus_tag="VLE22_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13412.1"
                     /translation="MTRISVSLLVLVLFGSAAFGAAGTPVAGKWACNDGAGHEAAWTL
                     VVKDDGAELTGTLIGGPTEPVEIPLVDPKFGESMFTFKINVNPNCVVEARLKLDANKL
                     EGPFGCPEAKGTLKAVRQP"
     gene            complement(3867..4334)
                     /locus_tag="VLE22_03075"
     CDS             complement(3867..4334)
                     /locus_tag="VLE22_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13413.1"
                     /translation="MAGYLERYGAGEEQRERRIRRVLLSIAVIAALAGTFYLLFRNYR
                     ETKQAKRFFELLLEKDYKAAYVLWGCDKPGAPACHDYTFDNFMEDWGPKSPHADLSSL
                     EFGKVRGCSTGVIVTVEFGKGREDLLWVDRDTKVIGFAPWPVCNPRLPASGTL"
     gene            complement(4353..4970)
                     /locus_tag="VLE22_03080"
     CDS             complement(4353..4970)
                     /locus_tag="VLE22_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSB13414.1"
                     /translation="MAPRPFSAGGGKSRCVNLSEYSQKVRSLFGMISWNAFRLPLLAA
                     VTCAFTVLPAAAQSKIAIINMQQALLATAEMKKAQAELEAKFKPRQEAMDKLRQELEG
                     IQKQLQTMGDKLTQQAQQDLNIQGQRKQRELQRIGEDLQSEVDFERNTILGKGGRQMA
                     DVVGKIAEEKGLDVVIDVSNAVYFKPALEITKDATDAYDKAYPVK"
     gene            complement(4972..5262)
                     /gene="groES"
                     /locus_tag="VLE22_03085"
     CDS             complement(4972..5262)
                     /gene="groES"
                     /locus_tag="VLE22_03085"
                     /inference="COORDINATES: protein motif:HMM:NF001527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSB13415.1"
                     /translation="MDIRPLHDRVLVQRTEESESVQHGIIIPDTAKEKPTRAKVIAVG
                     NGKLLESGERVPVAVKEGDHILFGKYSGSEIKIAGEEYLIVREDEILGVFSE"
     gene            5427..6521
                     /locus_tag="VLE22_03090"
     CDS             5427..6521
                     /locus_tag="VLE22_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HSB13416.1"
                     /translation="MAKKSVSRRSFMAVAGGVPAAATARNPVVANPLVISEVKVEQEP
                     LRIVTMYPFEPHEATKITSAGAVRVDLIICKTPEEFREKLRDAEVVYGGVRGRDLPGA
                     PKLKWVQAGGAGVEGLDREFVESPVVLTNYARTFAPGISETAMGLLLCLTRGISKYYM
                     PQFSKRTMEPVGTPKSDHHSELVGRTMGIVGMGGIGSAVARRAHYGFDMRIVGTDARP
                     IPKPEYVAELRDPEWFPKMVPQADVLVAAAPHTPETDRMFNEKVFRNMKKTAYFLALS
                     RGGLFDDMALVKALKEGWIAGAGLDVFPKEPPPSEHPIFDCPNVVMSAHTSGWSPDRQ
                     VRLIDLFAENVSRYTRGQPLINVVDKQRGY"
     gene            complement(6515..7252)
                     /locus_tag="VLE22_03095"
     CDS             complement(6515..7252)
                     /locus_tag="VLE22_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13417.1"
                     /translation="MISKPPLQFYSEPLPGIEPDELQGKLIVIEGPDAVGRSTQVGRL
                     RVWLEQKGHAVLDTGMARSALAGKGIQQAKEGHTLGSVTLTLFYTTDFADRLENKIIP
                     ALRAGFVVLTDRYIFSIMARAIARNEDRRWIEQVAGFALVPHAVFYLRCDVKDLVSRV
                     VLGRGAFDYWESGMDMRFGRDMYDSFVRYQTRLIRAFDRMASKYNFVTIDASQQPELI
                     FRRLQQHIGGLFTAPRTRPRQKSEFDQ"
     gene            complement(7221..7952)
                     /gene="tmk"
                     /locus_tag="VLE22_03100"
     CDS             complement(7221..7952)
                     /gene="tmk"
                     /locus_tag="VLE22_03100"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSB13418.1"
                     /translation="MALFLPNRFKGKLIVVEGIDGSGKSTQVSLLSQWLRSQGLAVAF
                     SEWNSSPLVSETTRRGKKKEMFTPTTFSLIHATDFADRMERYILPLLKAGAVVCADRY
                     AYTAFGRDVVRGVSRRWVRNLYRFAIRPNLAFYFRVPLDVALGRILGGRNAIKYYEAG
                     MDIGLSRNIEESFRLFQGRILEEYESMIAEMGFHVIDASGSIEDQQKQMRRIVMEELG
                     ERLRTDVLRSEPGETSDFETAASVL"
     gene            7985..9517
                     /locus_tag="VLE22_03105"
     CDS             7985..9517
                     /locus_tag="VLE22_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HSB13419.1"
                     /translation="MPRYAAIDIGSNSVRMLAAELAPGAPTRILAAERQVTRLGEGVF
                     RNGFLTSEAMDFACATLTSMARIYQQLDVVGVRAVATSAVRDARNQVEFLQRASDAIG
                     APVELISGQEEARLIHLGVQARWPHPKQRVLIIDIGGGSAELILSEQGRMTAAFSRPL
                     GAVRLTEAFLKHDPPEPAELHRLDEYIEEKLAGPIQRIGTGPWNRAIATSATASAVVC
                     AVNRIPRSRRDSADRLRATVPQVRKFFDAVSRRDLNARRKLTGIGPRRAEIIIPGAAV
                     LMRAMRDFHLPPIWYSDAGVRDGVIADLAARRVGEELTQLSREQRRVVEDTARHYGVQ
                     VKHARKVASLAHTLFDSFQSLHELPPNTGRLLEAAAYLHDTGHYVSDTRHHKHSYYLV
                     ANSDMPGFTDRERLLIANLCRYHRRALPAPVHDNLQLLSAEERRTVDLLHPLLRLADS
                     LDRSHDQRVENLECQIHDNTVVLHIRSAADTDLEEWAGEKAAEIFHKVYQRPLSLVRV
                     RS"
     gene            9518..9955
                     /locus_tag="VLE22_03110"
     CDS             9518..9955
                     /locus_tag="VLE22_03110"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HSB13420.1"
                     /translation="MKPGGSPCTGEPIAKYAAVQASRRLNRFAFELRRAAKSGDADAI
                     HDLRVSIRRLSQCLRVFGQFFPVRDSRKIRRRLRLIMDMAAELRNRDITLTLLNEAGA
                     GADTPLCRKIAEDRKEAERELAGSIRRWGRREFSAKWRRRLRI"
     gene            9960..10448
                     /locus_tag="VLE22_03115"
     CDS             9960..10448
                     /locus_tag="VLE22_03115"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HSB13421.1"
                     /translation="MAKKNAQKWKASLSAAENARIVLPLLSRDYFSAGRKAVGDKLPA
                     DELHQFRLETKQFRYTLEMFQSCYPAGLDQRLAQLRQIQQYLGDISDCLTARDLIRTI
                     QPRRSRQAGAVRAFLDQRAAELAAKFRDYWTSTFDAPGRERNWTDYLTRFAGRGRMRS
                     HL"
     gene            10549..10622
                     /locus_tag="VLE22_03120"
     tRNA            10549..10622
                     /locus_tag="VLE22_03120"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10581..10583,aa:Gln,seq:ctg)
     gene            10650..11462
                     /locus_tag="VLE22_03125"
     CDS             10650..11462
                     /locus_tag="VLE22_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13422.1"
                     /translation="MRLITFVFTVLSVAGLAQEPAELFEKAPPDVDAALRARITKFYE
                     AYVSGKFREAYEMVAEDSKEVFFEAEKSRIQQFTIVSIKYSDNFTKAAAVVACDRDFG
                     AMGTRFKVKVPVGSQWKIENGEWRWFAIHSDVVMTPHGPMHPGPMPKEDEVAKLTRAP
                     RVPVLKRMVQPDRQVVKLSSTKISTDQIQLKSALPGEVSLEMDRSQLLPGLEVEFDHM
                     QLEPKGTALLSFRYTPSEKKPPKTKEVRIVVRPTEQVIPLKVLFDEPGDAPK"
     gene            11530..12168
                     /locus_tag="VLE22_03130"
     CDS             11530..12168
                     /locus_tag="VLE22_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13423.1"
                     /translation="MSALTLPSASRQAKAEEAEPADNSFTEQLFLQLQRRFQRVGIEG
                     ERHALAAHSTRQNSVTGAKLSTAPVSFSANAKANLEPNYLSEEEAKTGGIALDPVSGE
                     TRTFSVNRYAFVDRATAERLAEMLGGTLETRQVGENGGPFRGPVQYVIRVGKYAGDAA
                     AISRRYESFPHWMAELSTRMAIARANGERTPTWNKALAQLNSGQAVFGVRLA"
     gene            complement(12202..>12639)
                     /locus_tag="VLE22_03135"
     CDS             complement(12202..>12639)
                     /locus_tag="VLE22_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13424.1"
                     /translation="ETTINDVPVKRYQPGLFETLDAEGKRFAVLLNEDGSYVTRENGA
                     KRGTKVRMFASGLGQTNPALNTNYAGIAGQSVLAKVVIGVNNAGVPVVAAETMPGVVG
                     VYMVTFDLPADTAAGPSRPFAIAIFPEDGGSLIFGNPSEIPVQ"
BASE COUNT         2554 a         3714 c         4001 g         2370 t
ORIGIN      
        1 gggctgccag acgcacaccg cccgcgctgg cacatccgaa cgaccgtatg gggaggtttg
       61 cgcccatgaa acagttgtgg tgtgtttcca cgctgacctt gttcgccgct ctgggatcgg
      121 ttgcggccga atccgatgca caagaggcac aagacgacaa ggcgaggcaa tggtccgtcg
      181 gaccggtccg gttcaaagga ctgatcgacg ggtactacag tctgaacctg aaccacccgg
      241 aatcgagaca gaaccagttg aggaacttcg acgtcaaggc caaccagttc agcctgaata
      301 tggctgagct ggtcctgcaa cacgatccgg agccgttggg tttccgggtc gatctgggat
      361 tcgggcgggc gttcgacatg attcatgcga ccgagccggc gcccaacgtc ttccggaata
      421 tcctgcaagc ctacgtaagc tacaaaccct cgaagaaaag cgatttccag gtcgactttg
      481 gaaaattcgt cacgggcgcc ggggccgagg tggtggagac tcacctcaat tggaattact
      541 cgcgctcgct tctgttcgct ttagcgattc cgtactacca tttcggcgcg cgcgcgaccg
      601 tgccgttgca tcatcacttc acggccggag cgatgattgt gaacgggtgg aacaacgtcg
      661 aggacaacaa ctccgggaag accgtcggcg taaccgggac gtttcattcc ccgtggttca
      721 catggaccaa caactactac gtgggtccgg agtggccggg ggtggatcac ggccgccgtc
      781 acctgtacga cacaacgttg ctgttcaatc cgtcggcgaa ggcggcgttt tacgtgaact
      841 tcgactacgg agcggagaaa aatccggctg gcaactcacg ctggctggga gtggcagggg
      901 ccgcgcgact ggcgctgtcg gatcgctttg cggtcacgcc gaggatcgaa tggttccacg
      961 acgccaacgg attcgccacc ggaacggccc agagactcaa ggagttcact ttgaccggag
     1021 aatacaaggt ggcctccgga gtgcttacgc ggctggagta ccggcgagac tggtcggacc
     1081 aaccgttctt cgagaggggc tcgacgcccg cgactttccg taatcaggct acggtgctca
     1141 tcggtttcgt tgccttcttc ggaccggagc gctaggagac tccggcaggt caggagagct
     1201 gtcccactca ggcctctgaa aatgatggct cgggccacgt caactaaagt ccgcaattta
     1261 gtaaactggg agtatctggg ttgctccaag catgaaaaca aatacagtga tctccgtgtc
     1321 cgcggttttt cttgtggcga tcgcatttgc gtttcagaaa tccgtcaaac tgccgccacc
     1381 cttccacagt ccgtcctcca acaatccgcc gcgagtgatc tcgcgcccgg acggcgcgca
     1441 attacggctt ccggctggat tcgacatcga cgagtatgcc ggtggtttcg agcggccccg
     1501 attcatgctg caggggccgt ccggcgagat cgtgctaagc gatagcgtgg agggcgggtc
     1561 cgtctacgtc ctgctcgaca agaacaatga tttcaaggca gacgagcgga acgtgctgct
     1621 ccgcgagctc gaccgcccct acggcctagc gttttggaaa gactaccttt acgtagcgga
     1681 gacgacgtcg ctgaagcggt acaagtatga ccgcaagaca atgacggccg gacgcggcga
     1741 ggaggtcgtc tcgatgaagg acttcgggtc aggccactgg acgcgcacgg ttctgttcga
     1801 ccgcaaaggc gagaagatgt accttgggat cggatcgcgt tcgaacgtat ctccggggga
     1861 gccggagagg cgcgccgcga tcagccggtt caatcccgat ggaagcggct acgagttgtt
     1921 tgcctccggg acaaggaacc caatcggcct ccgttggtac cccggcacgg atacgctttg
     1981 ggcggcggtg caagagcggg atgcgctcgg cgacgacctg gtaccggatt acttcaccac
     2041 aatccagcat ggtggcttct acgggtggcc gttcgcatac atcgggccga acgaggatcc
     2101 gcgcaacaag gggcagcgcc ccgacctggt gaagaagacg atcgtgcccg acgtgccgct
     2161 ccagtcgcac gtcgccgtgc tggactgtct gttctatacc ggcaagcagt ttccggcgga
     2221 gtaccgcggg ggtgcgtttc tcgcgtttca cggctcatgg aaccgctcga agcgggtggg
     2281 gtattctgtg actttcctgc cgtacaaggg tagcaaaccg agcgggccag tccgagattt
     2341 tttgacaggc tggatgctgg caccagacaa gcgagaggtc tgggggcgcc cggtcgggct
     2401 actggagttg ccggacggca gcctgctggt ctcggacgac ggcggcaaca aggtttggcg
     2461 aatttcgtac aaaggttagc cctcggcgcc gcagtggcgc tcgctgcgag tgtgcccgct
     2521 gtcccggcgg accgcgacgc cgagaggcgg ccattctcgc accgcctgca tctgaagctg
     2581 aggctggact gccagacgtg tcacgtttcg gccgcgcgga gcagccgcct ggaggataac
     2641 aacctcccac cgaaggaagt ctgtctccgc tgtcacaagc aggcagtcgt caaggcgccc
     2701 cgcaccaccg cgatcgcacg atttgaccac tcgaagcaca tcaaaatcgg agacatcggc
     2761 aaggtgatcg ccgccgcaat cgaccagggg acctaccttt caagcgcaga aggactgaag
     2821 caaaagctca cgagcggaca accttgcaaa ggttgccatc gggggctgga ggaatcggat
     2881 gaggtgacca aggcagcatt cccaacgatg gcggattgcc tcgtgtgcca ctccaagatc
     2941 cagcctcccg atagttgcct gttctgccac aacgaagggc agaagctcaa acctgacgac
     3001 catacgccgg actacatcga cctgcacaca ggcggaaaga tcagcctgaa gaaagaatcg
     3061 tgcgcggtct gtcacggcaa gcggttcaca tgcatgggtt gccactgacc gttggttcgg
     3121 cgcagcagcg agagttcacg gttgcctgac ggctttgagt gtgcccttag cctctggaca
     3181 cccgaatggg ccctccagtt tgttcgcgtc caacttaaga cgggcctcga ccacgcagtt
     3241 cgggttcaca ttgatcttga aagtgaacat gctttcccca aacttcggat cgaccagcgg
     3301 gatttcaacc ggctcagtcg gtcccccgat aagcgttccg gtgagttcgg ccccgtcgtc
     3361 tttcacaacg agagtccagg cagcttcgtg gcctgcgccg tcgttgcagg cccacttgcc
     3421 ggccacgggg gtaccggccg ccccgaacgc ggcggaaccg aatagaacca acaccagaag
     3481 cgaaacagat atcctcgtca tcactattct cctgaaaccg agttcgcaca agattccgct
     3541 ccgaaccgcg ccctgcgaag gctggcgtcc gcgagtgcag cggcgcggag cactactccg
     3601 gtgcaaggca gttcttccgc cagaaaagga tataggcgtc cggtttcaag cagcttgaca
     3661 aacaatccgc acggcaagtc gacagaatcg ccttctggcg acgcaagttg ctgacgttaa
     3721 agcagaaaga gattgtgggc actcaggtcc acgcgttacc cgaaggactc acggaaggct
     3781 gtcaaaccac ggacggcgca tgaccctgcc tgggctggtg accgctattg ggactgcatt
     3841 gcccggaacc gggcgctcat ccgccctcag agcgtccctg aggcaggcaa gcggggattg
     3901 cagacgggcc agggggcgaa gccgatcacc ttcgtgtcgc ggtcgaccca aaggaggtcc
     3961 tcccgaccct tgccgaactc gaccgtgacg atgaccccag tcgagcaacc tcggactttg
     4021 ccgaactcca gggaggagag gtcggcgtgg gggctcttgg gtccccagtc ttccatgaag
     4081 ttgtcgaaag tgtagtcgtg gcaagccggg gcgccaggct tatcgcagcc ccaaagtacg
     4141 taagcggcct tatagtcctt ctcgaggaga agttcgaaga agcgcttggc ctgcttcgtc
     4201 tcgcggtagt tgcgaaacag gagataaaaa gtgccggcga gagccgcgat cacagcgatg
     4261 gaaagcagaa cacgcctgat gcgccgctcg cgctgttctt cgccggcgcc gtagcgctcc
     4321 agataacccg ccatcgtcgt cagattctgt ttctacttta ccggatacgc cttgtcgtaa
     4381 gcatccgtgg cgtctttagt gatttccagg gcgggtttga aatagacggc gttggagacg
     4441 tcgatcacga catcgagacc tttttcttca gctatcttcc caaccacatc ggccatctgc
     4501 cggccacctt ttccgagaat ggtgttgcgt tcgaaatcga cttccgactg gaggtcttcg
     4561 ccaattcgct gaagctcacg ctgcttgcgc tggccctgga tgttgaggtc ctgctgagcc
     4621 tgctgggtga gcttgtcacc catggtctga agctgcttct ggatgccttc cagctcctgc
     4681 cgcagcttgt ccatcgcctc ctgccgaggc ttgaacttgg cctcgagttc ggcctgggcc
     4741 ttcttcattt ccgcggtagc caacaaggct tgctgcatgt tgatgatcgc tatcttcgat
     4801 tgcgcagcag cgggaagcac cgtgaaggcg caggtgacgg ccgccagcaa cggcagacgg
     4861 aaagcgttcc acgaaatcat gccaaacaga ctcctcactt tttgggaata ctctgacagg
     4921 ttaacacaac gtgacttacc accaccggcg gagaacgggc gcggcgccaa actattcaga
     4981 gaacaccccg aggatctcgt cctcgcgaac gataaggtac tcctcgcctg cgatcttgat
     5041 ttccgaaccg gaatatttgc cgaacaggat gtgatcgcct tccttaacgg ccaccggcac
     5101 acgctccccg ctttcgagca gttttccgtt gccgaccgcg atcactttgg cccgggtggg
     5161 tttttccttg gcggtgtctg ggatgataat tccgtgctga acggattccg attcttctgt
     5221 gcgctgaacg agcacacggt catgcagagg ccgaatgtcc atgagacgac tcctttcctc
     5281 gaagaagctc cttggaagac ggaatgggcg gcgatcaatg ccgtccgtcg tgtgccaata
     5341 tagcccgtgg gcgcggaaga gtcaacgccg gagggatgcg tgatattctt gtgagctgcg
     5401 cggcgcggcc gcgcgggagg ttccggatgg cgaagaaatc cgtttcgcgg cggagtttca
     5461 tggcggttgc cggcggggtc cctgccgccg cgacagcgcg caatcccgtg gtggcgaatc
     5521 ctttggtcat cagtgaggtg aaagtggaac aggagcctct ccgaatcgtc acgatgtacc
     5581 cgttcgagcc gcacgaggcg acgaagatta cgagcgcggg ggcggtcagg gtcgatctga
     5641 tcatctgtaa aacgccggag gagttccgcg agaaactgag ggacgcggaa gtcgtctacg
     5701 gcggcgtgcg cggacgggac ctgccgggag cgccaaagct gaaatgggtc caggcgggcg
     5761 gcgccggcgt cgagggcctg gaccgggagt tcgtagagag cccggtggtg ctgacaaact
     5821 acgcgcgcac gttcgctccc ggtatttcgg aaacggcgat ggggctcctg ttgtgcctca
     5881 cgcggggcat cagcaagtac tacatgcctc agttttccaa gcgcaccatg gagcccgtcg
     5941 gcacgccgaa gtcggatcac cattccgagc tggttgggcg gacgatggga atcgtgggaa
     6001 tgggcggtat cggcagcgcg gtggcgaggc gggctcatta cggcttcgac atgcgcatcg
     6061 ttggcaccga cgcacggccg atcccgaagc cggagtacgt tgcagaactg cgcgacccgg
     6121 agtggtttcc gaagatggtc ccgcaagcgg acgtgctggt ggcagcggcg cctcacactc
     6181 ccgaaacgga ccgcatgttc aacgaaaagg tcttccgcaa catgaagaag accgcttatt
     6241 ttctggcgct gtcccgcggc gggttgttcg acgacatggc gctcgtgaag gcactgaagg
     6301 aaggctggat tgccggcgcg gggttggacg tattcccaaa ggagcctccg ccgtcggagc
     6361 acccgatttt cgactgcccg aacgtcgtca tgtcggcgca cacctctgga tggagtccgg
     6421 accggcaggt gcgtctgatc gatctgttcg cggagaacgt gagccgctac acgagagggc
     6481 agccgctcat caacgtggtc gacaagcagc gcggctactg atcgaattca ctcttctgcc
     6541 ggggccgcgt ccgtggggcg gtgaagaggc cgccgatgtg ctgctgaagg cggcggaaga
     6601 tcagttccgg ctgctggctg gcgtcgatgg tcacgaagtt gtacttggat gccatccgat
     6661 cgaacgcgcg tatgagccgg gtctgatacc tgacgaagct gtcgtacatg tcgcgcccga
     6721 atcgcatgtc catcccactt tcccaatagt cgaaggcgcc tcggccaagg accacgcggg
     6781 aaaccaggtc cttgacgtcg cagcggaggt agaagactgc gtgtggtacg agagcgaaac
     6841 cggccacctg ctcgatccac cgccgatcct cgttgcgcgc gatggcccgc gccatgatcg
     6901 agaagatgta gcggtcggtg agaacgacga aaccggcgcg caaggcggga atgattttat
     6961 tctcgaggcg gtccgcgaag tcggtcgtgt aaaacagggt cagcgtgacc gacccgagcg
     7021 tatgtccttc cttggcctgc tggatgccct tgccggcgag agcggaacgc gccatgccgg
     7081 tgtcgaggac ggcatggcct ttctgctcga gccagacacg caggcgaccg acctgagtgg
     7141 accgcccgac cgcatcggga ccctcgatca cgatgagttt tccctggagt tcgtccggct
     7201 cgatgccggg cagcggctca ctatagaact gaagcggcgg tttcgaaatc actggtctct
     7261 cccggctcgg agcgaagcac gtccgttctc aggcgctcgc ccaactcttc catcacaatc
     7321 cggcgcatct gtttctgctg gtcctcaata gagccggagg catcgatgac gtggaagccc
     7381 atctccgcga tcatcgattc gtactcctcc agaatccggc cctggaacag ccggaagctc
     7441 tcctcgatgt tacggctcag gccgatgtcc atgccggcct cgtaatactt gatggcgttg
     7501 cggccgccga ggatccggcc caaggcgaca tcgaggggca cccgaaagta gaaggcaagg
     7561 ttcggacgga tggcgaagcg gtacaagttt cgaacccagc ggcgactcac gccacggacc
     7621 acatcccggc cgaaagcggt gtaggcgtag cggtcggcgc aaacgacggc gcctgctttg
     7681 aggagcggaa ggatgtagcg ctccatgcgg tcggcaaagt cagtggcgtg gatgaggctg
     7741 aacgtcgtcg gcgtgaacat ctccttcttc ttgccgcggc gggtggtttc gctcaccagt
     7801 ggagaggagt tccattcact gaaggcaacc gccagcccct gggagcgcag ccactggctc
     7861 aggagcgaga cttgggtcga cttgccggag ccgtcgatgc cttccaccac gatgagcttg
     7921 cccttgaagc ggttcggcag aaagagagcc ataagcaccg gggaattctt attatattcc
     7981 tagtatgccg agatacgccg cgatcgacat cggcagcaat tctgtgcgga tgctcgccgc
     8041 ggaattagcc ccgggtgcgc cgacgcgcat ccttgcagcc gagcgtcagg tgacccggct
     8101 gggagagggc gtttttcgga acggatttct cacctccgag gcgatggact ttgcctgcgc
     8161 gacgctgacg agtatggcgc ggatttacca gcagctcgac gtggtgggtg tacgtgccgt
     8221 agccaccagc gccgtgcggg acgcacgcaa ccaagtggag ttcctccagc gggcttccga
     8281 tgccattgga gcgcctgtgg agctgatctc cgggcaggag gaggcgcgcc tgattcacct
     8341 cggcgtgcag gcccgctggc cgcatcccaa gcagcgcgtg ctgatcatcg acatcggtgg
     8401 cggcagcgcg gaactgatcc tgagcgaaca aggccggatg acggcggcat tctccaggcc
     8461 gctcggcgca gtgcggctga cggaagcgtt cctcaaacac gacccgccgg aaccggccga
     8521 attgcaccgg ctagacgagt acatcgaaga gaagctggct ggaccgatcc agcggatcgg
     8581 gaccggcccg tggaaccgcg cgatcgcgac gtctgccacc gcgtccgcgg tagtgtgcgc
     8641 ggtaaaccgc attccgaggt cgaggcgcga ctcggccgac aggttgcgag ccactgtccc
     8701 gcaggtgcgc aagtttttcg acgctgtctc gcggcgcgac ttgaacgcac gccgcaaact
     8761 caccggtatc ggaccgcggc gagccgagat catcattccg ggcgcggcgg tgctgatgcg
     8821 ggcgatgagg gatttccacc tgccgccgat ttggtactcc gacgccggtg tccgagatgg
     8881 agtcatcgcc gatctggccg cacggagggt gggcgaggag ctcacgcaac tcagtcggga
     8941 acagcgcagg gtggtcgagg acacggcgcg gcattacggg gtccaggtga aacacgcgcg
     9001 caaagtcgcg tcgctcgcac atacgctgtt cgattcgttt cagtcgctgc acgagcttcc
     9061 gccgaacact ggaaggctgc tcgaagcggc cgcctatctg catgacaccg ggcactatgt
     9121 cagcgacact cggcaccaca agcactccta ttacctggtg gcgaactccg acatgcccgg
     9181 gttcaccgac agggagcggt tgctgatcgc aaatctgtgc cgctaccaca ggcgcgcgtt
     9241 gccggcgccg gttcatgaca atctccagtt gctaagcgcc gaggaaaggc gtacagtcga
     9301 tctgctgcat ccgctgctgc ggcttgcgga tagcttggat cgcagccacg atcagagggt
     9361 cgagaacctg gaatgtcaaa ttcatgacaa cacagtggtg ctgcatatcc ggtcggctgc
     9421 ggacacagat ctggaggagt gggcgggcga gaaggccgcg gagatatttc acaaggtcta
     9481 ccagcgcccg ctgtcgctgg taagggtcag gtcataaatg aagccggggg gtagcccttg
     9541 caccggtgaa ccgatcgcga aatacgccgc tgtccaggcg tcccggcggc tcaaccgttt
     9601 cgcgttcgag ttgcggcggg ctgccaagtc cggcgatgct gacgcaatac acgaccttcg
     9661 cgtgtcgatc cgccggctct cgcagtgtct gcgcgtcttc ggacagtttt ttcccgtgcg
     9721 cgattcaagg aagatccggc ggcggcttcg cctgattatg gacatggccg cggaactccg
     9781 caaccgcgat atcactttga ctctcctgaa cgaggcgggc gcaggcgcag acacgccatt
     9841 gtgccggaag atcgccgagg accggaaaga agcggaacgg gagctcgccg gctcgatccg
     9901 aaggtgggga cgtcgagagt tctcggcgaa atggcgccgg cggctgcgta tctgacgtca
     9961 tggcgaagaa gaatgcccag aaatggaagg cgtcgcttag cgccgcagag aacgcgagga
    10021 tcgtgctccc attgctttcc cgagactact tttcagcggg gcgcaaggcc gtcggggata
    10081 agctgccggc agacgagttg caccagttca ggttggaaac gaagcaattt cgctacacgc
    10141 tggaaatgtt ccagtcctgc tatcccgcgg gcctggacca gcgactggcg caactgcggc
    10201 agatccagca ataccttggc gatatcagcg actgcctcac tgcgcgggat ctgatccgga
    10261 cgattcagcc gcggcgatcg aggcaagccg gggcagtccg tgcgttcctc gatcagcggg
    10321 cggctgagct ggccgccaag tttcgtgact actggacttc gacgttcgac gcgccgggac
    10381 gggaaagaaa ctggaccgat tacctgacgc gattcgctgg ccgcgggaga atgcgatcac
    10441 atctctgacc ggcgctccgg ccgaaacgaa ggagtggccg gaacgtccaa tgatcaatgg
    10501 cggacaaccc aatttccgga ctgtgcgatg ctaaaatagt gagggaattg ggaggtcgtc
    10561 taacggtaag acggcagact ctggatctgc ttatcggggt tcgaatccct gcctcccagc
    10621 caaggacttt accgcgtttc tgttctccca tgcgactcat tacctttgtt ttcactgttc
    10681 tgagcgttgc tggtctcgct caagagccag ccgaactctt tgagaaagcg ccgccggatg
    10741 tagatgcggc gctgcgcgcg cgcatcacca agttctatga agcatacgtg agcggcaagt
    10801 tccgcgaggc ctacgaaatg gtcgctgagg acagcaagga ggtctttttc gaggccgaga
    10861 agtcgcgcat tcaacagttc actatcgtca gcatcaagta ttccgacaac ttcacaaagg
    10921 ccgcagcggt cgtcgcctgc gaccgtgact tcggtgccat ggggactcgt ttcaaggtga
    10981 aggtaccggt gggcagccaa tggaaaattg agaacggcga gtggcggtgg ttcgcgattc
    11041 attccgacgt ggtgatgacg ccccacggtc cgatgcaccc tgggccgatg ccgaaggaag
    11101 atgaagtcgc gaagttgaca cgcgctccgc gggttccggt gctcaagcga atggtgcagc
    11161 ccgacaggca ggtggtgaaa ttgagcagca ccaagatctc gactgaccag atccaactca
    11221 agagcgcgct ccctggcgag gttagcctgg aaatggatcg gagccagcta ttgccggggc
    11281 tggaggtcga gtttgaccac atgcagttgg agccgaaggg gacggcgctg ctctcgttcc
    11341 gatacacgcc ctccgaaaag aagccgccaa agacaaaaga agtccgcatt gtggtccggc
    11401 cgaccgagca ggtaattccg ctcaaggttc tgttcgacga accaggcgac gccccgaagt
    11461 agttggcacg gcggttgctg aagcctcagc ggaggtttca gtcctatgtt ccgaggagtg
    11521 tccggtcccg tgtcagcgtt gactcttcca tcagcctcgc gtcaggcgaa ggcggaggaa
    11581 gccgaaccag ccgataactc cttcactgag cagcttttcc tgcaacttca acggcgtttc
    11641 cagagggttg ggatagaggg ggaaaggcac gcgctggcgg cgcactcgac ccgccaaaat
    11701 tccgtcactg gcgccaagct ttcgacagct ccggtgtcct ttagcgcaaa cgcgaaggct
    11761 aacctggaac caaactatct gagcgaggag gaggcaaaaa ccgggggcat cgccctggac
    11821 ccggtgagcg gtgagacgag gaccttctca gtcaaccgct atgcgttcgt cgatcgggcc
    11881 acggcggaac gtctcgccga gatgcttggg ggcacactgg agacccgcca ggttggggaa
    11941 aacggaggtc cattccgcgg gccagtacag tatgtaatcc gagtgggcaa atacgccggc
    12001 gacgccgcgg caatctcccg gcgctacgaa agcttcccac attggatggc cgaattgtcg
    12061 acccgcatgg cgatcgcgag ggccaacgga gagagaactc cgacgtggaa caaggccctg
    12121 gcgcagctaa acagcgggca ggccgtgttt ggagtgcggt tagcataggg gcacggcgcg
    12181 ggccgtgccc ctaccgaggg cttactgcac cgggatttcc gacgggttgc cgaagatcag
    12241 cgacccgccg tcttccggaa agatggcgat ggcgaacgga cgcgacgggc ccgctgccgt
    12301 atcagcgggc aagtcgaacg tcaccatgta gacgccgacg acaccgggca tggtctcggc
    12361 cgctacgacc ggcacgccag cgttgttgac gccgatgacc accttggcga gaacgctctg
    12421 tccagcaatc cccgcgtagt tggtgtttaa cgctggattc gtctggccca gacccgaggc
    12481 gaacatccgc accttcgtgc cacgctttgc gccgttctct ctggtgacgt agctaccgtc
    12541 ttcgttgagc agcaccgcaa agcgcttgcc ttccgcatcc agtgtctcga agagtcccgg
    12601 ctggtaccgc ttgaccggga cgtcattgat ggtggtttc
//
LOCUS       DATIFQ010000072         6066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1747519, whole genome shotgun sequence.
ACCESSION   DATIFQ010000072 DATIFQ010000000
VERSION     DATIFQ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747519"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1039)
                     /locus_tag="VLE22_03140"
     CDS             complement(32..1039)
                     /locus_tag="VLE22_03140"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB13425.1"
                     /translation="MRLLPVLLLCACGWAQSDPKALQILERVAAKYKNLDSFVMIGAA
                     TRPVFPDKVAQVKVQRAYVSRRFVPDDAPLPFVDAAARYRDDLEPAFLIFRDIADRVA
                     GVRLLRSETAILGGRPRPCDVLSVRYEDHARSSALKPVVYWIERETGTVWKLQFSERR
                     ASDVWQWTVALDQSSENEPPPSWAMLPAKMVTREDTRLAGSDAPEISGRTLDGRSFAL
                     SKLRGKVVVLDFWATHCGYCSEEMADLEQLKTSFTAQGVEIVGVNEEEPELAKRWLAE
                     RKRTLPVVLIAPETGFKSYGVDSLPTTVVIDRQGKVVKQWIEFASGRKVRRIIETLIA
                     R"
     gene            1159..1944
                     /locus_tag="VLE22_03145"
     CDS             1159..1944
                     /locus_tag="VLE22_03145"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSB13426.1"
                     /translation="MRKLFLVALVVSAAALMGQDRRRSAIDPRKVVDLSYPYDERTVY
                     WPTAEGFQRKEDAWGVSAAGYWYASASFCTSEHGGTHLDSPVHFAEGKWTTAEIPLER
                     LIGPAAVIDIAEPCLKDRDYRLSASDVLAWEKKHGRVAAGDIVLVRTDWGRYWPDAKK
                     YLGSEKRGDASGLSFPGLSREAAELLADRKVRGVGIDTASLDYGRSSDFIVHRTLNGA
                     NIYGLENVAQLDRLPPRGATLIALPMKISGGSGGPVRIIAVMP"
     gene            complement(1934..2428)
                     /locus_tag="VLE22_03150"
     CDS             complement(1934..2428)
                     /locus_tag="VLE22_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HSB13427.1"
                     /translation="MEYVLFALAIGLMVSNAFGVPAWLREAVRTEYYIKIGLVILGAG
                     MLFEEILQVGVLGVIQAVLVVSVVWQIGYWLSRKLQVDAEFAVILSTAVSICGISAAI
                     AACGAIQGDHKKLSYVTSLVLIVAVPMMVLMPWAVKRFGIPELVGGAWLGGEDHSHPA
                     VPKA"
     gene            complement(2432..2695)
                     /locus_tag="VLE22_03155"
     CDS             complement(2432..2695)
                     /locus_tag="VLE22_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13428.1"
                     /translation="MLAGLRPLMPRFSWSVPGSGGTAPLGQVFGSENLLRSALIGAGY
                     LVISDLGLAFMGASLARYLIGFPVLYSLSWLSLVIAGNSTVSY"
     gene            3140..3754
                     /locus_tag="VLE22_03160"
     CDS             3140..3754
                     /locus_tag="VLE22_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HSB13429.1"
                     /translation="MSARETGLDKARQVFAQHQGMLRTSEAIRLGVHPRTLYALRDKG
                     QIEQVARGFYRLSERPALTNPDLVAVALRIPRAVICLISALAHHGLTTQVPHTVDVAL
                     PGHAQIPNLGGIPLRVFWYSEPSFSAGVQSVSIDGVAVRIYSAEKSIADCFKYRNKIG
                     LDVAVQALRTYRERTRKPDLMLISKFARVDRVEKVMRPYLEAVL"
     gene            3751..4653
                     /locus_tag="VLE22_03165"
     CDS             3751..4653
                     /locus_tag="VLE22_03165"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HSB13430.1"
                     /translation="MSGRDLGASVRQRLLNRARAEGRPFQELLQYFAMERFLYRLARS
                     PFADRFVLKGALLLTAWKAPITRPTIDIDLAGRTSNQLEHIRAIVAKLCQLYTEPDGL
                     EFYPASIEGQRIKEDAEYEGVRIRFHATLAKARIPMQIDVGFGDVIVPKPVEVEYPAM
                     LEFPPPVLRAYPKETVVAEKLEALTVLGMLNSRIKDYYDLALLARLYPFEGALLVKAI
                     HSTFRHRGTTVEPEPVGLTEAFSSDPARAVQWRAFVRRSRLESGWELEKIVEQVRLFA
                     STPLAAAAADRPFDLNWRPGGPWE"
     gene            4833..5618
                     /locus_tag="VLE22_03170"
     CDS             4833..5618
                     /locus_tag="VLE22_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HSB13431.1"
                     /translation="MSSPLNFHLEHYEGPLDLLLDLIRKQEINIYDIPIASITAQYLE
                     YVQQALALDIELSAEFVYMAATLIHIKSRMLLPRDPELEKLYPEEDPRQELVERLLEH
                     ERFKQAAEMLQQKRLIEENVWSNPQISAFESDDDEPGLAVTMFDLVKTLQQVLERAKS
                     RPTYDVTDEDVSVPEMIQRLKSMLERTPRGTPLSAAEFFERQQNRRTMIAAFLAILEM
                     VKLQAVVLYQKDAFGEILIKRHKRFEEVFASGEAMAAIEKGYS"
     gene            5624..>6066
                     /locus_tag="VLE22_03175"
     CDS             5624..>6066
                     /locus_tag="VLE22_03175"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HSB13432.1"
                     /translation="MELTDQNAQVELDALLQEQPDATAPPAPDDSSESQLLAMLEAVV
                     YLTDEPLSAAQIAAALGQPADKVEKALERLVTEYEKPEHGVTLRHVAGGYKMTTKAEH
                     HEAIRGFVKNLKPPLKLSLPALETLALIAYKQPITAPEIMEIRGV"
BASE COUNT         1217 a         1953 c         1822 g         1074 t
ORIGIN      
        1 gtgctggact caacaggtta cacggtaccg gctaccgggc gatcagagtc tcgatgatcc
       61 ggcgtacctt tcggccgctc gcaaactcga tccactgttt gacgaccttc ccttggcggt
      121 cgatcaccac ggtcgtgggc aaggaatcca cgccatagct tttgaaaccc gtttcggggg
      181 cgatcaagac gaccggcagc gtgcgcttcc gttccgccag ccaacgcttt gccagttccg
      241 gctcctcttc gttgacgccg acgatctcca ctccctgtgc ggtgaaactt gtcttcagtt
      301 gttcgagatc tgccatttcc tcggagcagt aaccgcagtg cgtggcccag aagtcgagca
      361 cgacgacctt gccgcgaagc tttgaaagcg caaacgaacg cccgtcgagg gtccggccgc
      421 tgatttcggg agcgtcgctg ccggccagcc gggtgtcctc ccttgtgacc atctttgcgg
      481 gcagcatagc ccacgaaggg ggaggttcat tttcggacga ttggtcgagg gccaccgtcc
      541 actgccagac gtccgaggcc cggcgctcgc tgaactgcaa tttccatacg gtgccggtct
      601 cgcgctcgat ccagtagacc accggcttga gcgccgagct gcgcgcgtgg tcctcatacc
      661 gaacggagag gacatcgcac ggcctgggcc ggcctccgag gatggcggtt tcgctccgta
      721 gcagcctcac cccggccaca cggtccgcga tgtcgcggaa gatcaggaag gcgggttcga
      781 ggtcgtctcg atagcgtgcc gccgcatcga cgaacgggag cggcgcgtcg tcgggtacga
      841 agcgccgaga cacgtacgcg cgttggacct tgacttgagc aactttgtcc ggaaacaccg
      901 gccgcgtggc cgctccgatc attacgaaac tgtcgaggtt cttgtacttc gcggcgacgc
      961 gttccaggat ctggagggcc ttcggatcgc tttgcgccca gccacaggca cagagaagca
     1021 atacgggcag aaggcgcata ccgtcctccc gcacttatta taggacccaa gggtcttgaa
     1081 caacagaatc ccatctacag ggtggccacc atcctgggcg atgcactcgt gcgtcttctg
     1141 gtgtgggaaa atgcgcccat gaggaagctg ttccttgtcg cccttgtggt gtcggccgcg
     1201 gcgctcatgg ggcaggaccg gcgccgcagc gccatcgatc cccgcaaggt cgtggacctc
     1261 agttacccct acgacgagcg cacggtctac tggcccaccg ccgaagggtt ccaacggaag
     1321 gaagacgcct ggggcgtctc ggccgcaggc tactggtacg cttcggcgag tttctgcacg
     1381 agcgaacacg gtggcacgca ccttgacagc ccggtgcact ttgccgaggg caagtggacc
     1441 actgcggaga ttcccctgga acgcctcatc ggtcctgccg cagtcatcga catcgcggag
     1501 ccctgcttga aggatcgtga ctaccggctc agcgccagtg acgtcctggc atgggagaaa
     1561 aagcacggtc gcgtcgccgc cggcgacatc gtgctggtac gcaccgattg gggccgctac
     1621 tggccggacg ccaagaagta cctgggctcg gagaagcgcg gcgatgcgag cggccttagc
     1681 tttccgggtc tttcccgcga ggccgccgag ctgctcgcgg atcgcaaggt taggggagtg
     1741 ggcatcgaca cggccagcct ggactacggg cgttccagcg acttcatcgt gcaccggacc
     1801 ctgaacggcg caaacatcta cggtctcgag aacgtggccc agttggaccg gcttccgccg
     1861 cgaggcgcga cgctgatcgc cttgccaatg aagatcagcg gcggcagcgg cggcccggtg
     1921 cgcatcatcg ccgttatgcc ttaggcacag cggggtggga atggtcctca ccgcccagcc
     1981 aagcgccgcc gaccaattcc ggaatcccaa agcgcttcac ggcccagggc atcagaacca
     2041 tcatcggcac tgcgacgatc aacaccagcg acgtcacata ggagagcttc ttgtggtcgc
     2101 cctggatggc cccgcaggcg gcaatcgccg ccgagatccc acagatcgag acggcggttg
     2161 aaaggatcac cgcaaactcg gcatcgacct gcaacttcct ggaaagccag taaccaatct
     2221 gccagaccac gctcacgacg agcacagcct gtattacccc caacacaccc acctgaagga
     2281 tttcctcaaa gagcatcccg gcccccagga tcaccaagcc gatctttatg tagtactcgg
     2341 tccgcacggc ctcccgaagc caggccggaa cgccgaaggc attgcttacc atgaggccta
     2401 tggcgagcgc aaagaggacg tactccaagc ctcagtagct caccgtggag ttgcccgcaa
     2461 ttaccagtga aagccagctg aggctgtaaa ggacgggaaa gccgatcagg tatctggcga
     2521 ggctggcacc catgaaggcg agcccgaggt cggagatgac gaggtaaccg gcgccgatca
     2581 gggccgagcg caggaggttt tcacttccga agacctgacc caggggtgcc gtgccgccgc
     2641 tgcctggcac agaccagcta aaacgcggca tcagcggccg aaggccggcc agcaccaacg
     2701 cgatcaggag aaacgccggt cacacggcca accagtcttc acttgccaga aatgccgggc
     2761 gccttgcgtt gcgcgtcatg gggagactct actgcagatt ctcctccagc cgaaggggag
     2821 attccaatac gcgattctac tgttttcccg ccagccgcct ctcccggtcc ggcttgaaga
     2881 atctatgcgt tgtaagctat tgaaaagaat aggtctccga gaggccacgc gcgtcctaac
     2941 aggatatata gagaatagga agcccttccg tttctagcca ttaccaacca cgtcgaaccg
     3001 ttaccagggc tcggcgaaac ctctccgttc ccgctcctgc aaagggtacc gccttgcggt
     3061 tttcaatggg caccggttgc gtgccttgtg gtggatagaa gtatcggaaa acaagtataa
     3121 tgtccgtgga gtctatccag tgtcggccag ggaaacaggt ctcgacaaag cgcgccaggt
     3181 gttcgcccaa caccaaggaa tgctgcgaac cagcgaagcc atccgactgg gtgtccaccc
     3241 acggacgctg tatgcgctta gggataaggg ccagattgaa caggtggccc gcggcttcta
     3301 tcggctgtcc gaaaggcccg cgctcacgaa ccccgatctg gtggccgtgg ccctccgaat
     3361 cccgcgagct gtgatctgcc tgatctcggc cctcgcgcat cacggtctca cgacccaggt
     3421 tccgcacacg gtggacgttg ctcttcccgg ccatgcccag attccgaacc tcggtggcat
     3481 ccccctccgc gtgttctggt actccgagcc atccttcagc gccggcgtcc agtccgtctc
     3541 gattgatggg gtagcggtta ggatctactc agcggagaag tccatcgcag actgcttcaa
     3601 ataccgaaac aagatcggcc tcgatgtggc cgtccaagcg ctccgaacgt accgcgagcg
     3661 aacgagaaag cctgacctga tgctgatctc caagttcgcg cgagtggatc gagtcgagaa
     3721 ggtgatgcga ccctacctgg aggcggtgct gtgagcggcc gcgatctagg cgcatccgtc
     3781 cggcagcggc tactgaaccg cgcccgcgca gaaggccgac cgtttcagga actgctgcag
     3841 tacttcgcca tggagcgctt cctctatcgg ctggcgagat ccccgttcgc ggaccggttc
     3901 gttctgaagg gcgccctcct cttgacggcc tggaaggccc cgattactcg gccgacgatc
     3961 gacatcgacc tggccggcag gaccagcaac cagttggagc atatccgggc gatcgttgcc
     4021 aaactctgcc aactctatac cgaaccggac ggactggagt tttatccggc ctctatcgag
     4081 gggcaacgca tcaaggaaga cgcagaatac gaaggcgtgc gaatccggtt ccacgcgacg
     4141 cttgcgaaag cccgtatccc gatgcagatc gacgtcggct ttggcgacgt gatcgtgccg
     4201 aagcccgttg aggttgagta cccggccatg ctcgaattcc ccccaccggt gctccgggcc
     4261 tatccaaagg aaactgtcgt ggcggagaag cttgaagcgc tcacagtgct cggcatgctc
     4321 aacagccgga tcaaggacta ctacgacctg gcactgttgg cgcggctgta tccgtttgaa
     4381 ggtgcccttc tggtcaaagc cattcactcg actttccgcc atcgcggaac gacggtggaa
     4441 ccagaaccag tgggactcac agaagcgttc agctcagatc cggcgcgggc agtacaatgg
     4501 cgcgcctttg ttcgccgcag cagactcgag tcggggtggg agttggagaa gatcgtggag
     4561 caggtgcgac tctttgcctc cacaccgctc gccgccgccg ctgcagatcg tccttttgat
     4621 ctcaactggc ggccgggcgg accgtgggag tgactggctg tggcactgtc ccgcgcgtgg
     4681 ttgagtggac ccaaccctgg ggcaatccgt gccggcaacc cgtgccagtg accgtggctc
     4741 cgatcccgcg cgcccgctcc cccgattgtg gcgccgcccg ggttccggag gcccgcgagc
     4801 cgcccgttgc ctccgtgcta tcgtgagaag ttgtgtcgtc gccgttaaac ttccatctgg
     4861 agcattatga ggggccgctt gatctgctgc tggacctgat ccgcaagcag gagatcaaca
     4921 tctacgacat ccccatcgcc tccatcactg cccagtatct ggagtacgtg cagcaggccc
     4981 tcgcgctcga catcgaactg agcgccgagt tcgtctacat ggccgccacc ctcatccaca
     5041 tcaagtcgcg gatgctgctg ccgcgcgatc ccgagctcga aaaactgtat ccggaggaag
     5101 acccccggca ggaactggtc gaacgcctcc tcgagcacga gcggttcaaa caggccgccg
     5161 aaatgctcca gcagaagcgc ctcattgagg aaaacgtctg gtccaacccg cagatcagcg
     5221 cgtttgagtc cgacgacgac gagcccggac tcgccgtcac catgttcgac ctcgtgaaga
     5281 cgttgcagca ggtgctggaa cgcgcgaaga gccggcctac ctacgatgtg accgacgagg
     5341 acgtcagcgt gccggagatg atccaacggc tgaaatccat gctcgagcgg acgccccgcg
     5401 gcaccccgct ttccgctgcc gaattcttcg agcgccagca aaaccgccgc accatgatcg
     5461 ccgccttcct ggccatcctg gagatggtga agcttcaggc ggtggtcctt taccagaagg
     5521 acgccttcgg cgagatactc atcaagagac acaaacgatt tgaagaggtc ttcgcctccg
     5581 gtgaggcgat ggccgccatt gagaagggat acagctagct gggatggagt tgaccgacca
     5641 aaacgctcaa gtggagctcg acgcactgct ccaggagcag cccgatgcca ccgcgccccc
     5701 cgcgcccgac gattcgtcgg aatcgcagct cttggccatg ctggaggccg tcgtctactt
     5761 gacggacgaa ccgctgtcgg cggcgcaaat cgccgcggcg ctcggccagc cggccgacaa
     5821 ggtcgagaag gccctcgaaa gactcgtcac cgaatacgag aagcccgagc acggcgtcac
     5881 cctccggcac gttgccggcg ggtacaagat gaccaccaag gccgagcatc acgaggcgat
     5941 ccgcggcttc gtcaagaatc tcaagccgcc gctcaagctc tcgctgcccg cgctcgagac
     6001 gctcgccctg atcgcttaca agcagcccat cacggcgccg gagatcatgg agatccgcgg
     6061 cgtgca
//
LOCUS       DATIFQ010000073         8664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2129823, whole genome shotgun sequence.
ACCESSION   DATIFQ010000073 DATIFQ010000000
VERSION     DATIFQ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129823"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..969)
                     /locus_tag="VLE22_03180"
     CDS             complement(46..969)
                     /locus_tag="VLE22_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13433.1"
                     /translation="MRPLGLLLPFLLLGAAPPVANEKTIHVPLWVAAGGDSGLTAANV
                     SAKLEGRPATVRSLLGPDDPLLLLIVTDMVGDVSAIAPAKQALIEAVRQLPATAHAGL
                     LRAQDGLNVVLDPTADREALAAAIEAMTISGNAGMLNTVETIARIADGILQKSAVRVA
                     VLYVTDSDIRNYREDYTNPVINYSDPHDMSRRFPEGLIREKISKLDGVLARLEAPLFV
                     VHLDYRSETLNEAYQVGLKQLAATTGGASSFCRSNAEIPGAIKEMFRTILSYYRLDLQ
                     LPARYPKIVKVELSSDGRNLNYRTEFRLDGR"
     gene            complement(970..3762)
                     /locus_tag="VLE22_03185"
     CDS             complement(970..3762)
                     /locus_tag="VLE22_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13434.1"
                     /translation="MRSAFRLLITSLGCACAALSQQAEPGAEMTKAVEEFKVLTRDMG
                     LRPDSPRKRKQNGARWKWHGRVFENLRNDLLDAVPHEIVQLGGNKSLLRRNQFGFNIG
                     GPVVIPFLYSGTRSTHFSVSYEGVRERVSRSNLTTIATAPEHGGDFSATVDQSGVLLP
                     IFDPLSTRPNPNFDPSQAVSTENLEYLREQFPGNRIPAHQLDSVAQKALSYYPKPNAA
                     VGPFDRNNYFLVSPETNTANGMIAKVDHTLRERHRLNFDLSYSNGSLGAAELLPGPAN
                     PGANDRRFHSRRASVEHVFTSSSQTVNTLGFEATTDGSKNGSDTEADYPAELGLSGVS
                     GPTFPYLRIYPYLSMGRSYPISTNTRNSFSWSDSLSSRRGKHNLRLVTRHTRFQVHSY
                     WPQYPTGFLRFEPELTGLPGIVNTGHAFSSFLLGYAEYGARSIVVAPSYFRRSSTFVT
                     MRDSYELRRGFTISGGASMSCYTPRVEKYDRQSTIDLTAINPANDRPGALVVAGQGGH
                     GRAFQPTLVKVEPNLGLAWNPRGDTKTVVRANFSRDYSVIPVYYGQFGTQAFNATPTY
                     ISPNTQLQPALILTDGFPAPDHPVPDTRPEAANDTIADLINTTDNQPIYQSASLSLER
                     ELPGSFVLTAGLAYAGGKNLPVSENTADPNSLRLETLQYRDQLNDEEFNRSLRPYPQY
                     KGFELNDSWPLGRYRRDAGWLRVEKRTSRGLTLNLTYQVSKQYDDYSGPTGRQDVYNR
                     DNEWSLTWYNRPHYLGMNYVYELPFGRSLTDWRRLALEGWSLSGSSSVVSGEPLAIRP
                     LFNNTGGVVRALRVNVVPGADPSVANQGPDLWFNPAAFDQPADFTIGDAARTHPTLRA
                     PTNQNHDLALNKRFVLGADRTIELSAVGLNFLNHANWNDPDQYVGPASAPNLNAGKII
                     GSRGGRVIQVGVRLSF"
     gene            3814..4860
                     /gene="lpxD"
                     /locus_tag="VLE22_03190"
     CDS             3814..4860
                     /gene="lpxD"
                     /locus_tag="VLE22_03190"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HSB13435.1"
                     /translation="MKLKDIAARLECRLHGNGEIEITGLAGMERAGPSELTFLANQKY
                     IPKVKHTKAAAILVTEPVEGVPIASLVSRNPYFDFARALELFYRPPRPAPGIHPLAYV
                     SPSAEIGDGASIGPFAAVGDRVKIGRNAILHPHAVIYEGVEIGDDFTAHSHSSVREYC
                     RIGNRVILQNGVVVGGDGFGFAKREDGTHYKIVQSGPTVIEDDVEIQSLSNIDRATVG
                     ETRVKRGAKIDSLVQVGHACVVGEDNIICAQVGMAGSSILERNVLLAGQVGVSGHLTV
                     HDNAIVYAQSGVGGDVDPGAVISGSPAFDAVAWRRSVAAFQRLSDLVRTVRELKNRVA
                     QLESAAPSSTDSHE"
     gene            4853..5650
                     /locus_tag="VLE22_03195"
     CDS             4853..5650
                     /locus_tag="VLE22_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HSB13436.1"
                     /translation="MNNQNQARYRRPKAYKNERFLDSADARPLRILSEYLEPLSHFRR
                     EKIRDTIVFFGSARLHEDGPMGKYYNEARELARLLTEWSNQLENTSRRFVVCTGGGPG
                     IMEAANRGAQDANGKTVGLNIGLPFEQLPNPFITPELSFEFHYFFMRKFWFAYLAKAL
                     VVFPGGFGTLDELTELLTLAQTEKLAKKIIIVLYGTSYWREILNFEALVRHGMISEED
                     LDLFQFADDPQTALRILQDGLRKYYLEPGVPLPEREMETPAIAKSRI"
     gene            5752..6864
                     /locus_tag="VLE22_03200"
     CDS             5752..6864
                     /locus_tag="VLE22_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB13437.1"
                     /translation="MSQFNRRYFLMGSVAASIARPSMGQSANDALNVGMIGTGNRGYY
                     VMGGVMKQPGVKVVAVCDIKPDRLDKAATAASGAKTYTDYHKLLAQKDIDAVFIATPC
                     DLHVEMSIAALKAGKHVYCEKPVGITPESIRELVKAAKASNKVFQVGQQMRADARLRK
                     TIERIHEGVAGKVVMVKAQRHAGDDLDHDGSSADWFFNAARSGDVITEMSVHNLDVCN
                     WAIDSLPERAAGFGGALVWPNIPPGRTNMDGYALSYEYANGVKLSYTQVFFHPNGMPN
                     GGQYFNVFTTEGGVDLMSSTFYSRERGKKPVVLVPPENTPDDAHTAAFFEAIRTGSKP
                     FADIRVGATGALTAVMGREAIYRKKMMTWREMGVDI"
     gene            6877..7572
                     /locus_tag="VLE22_03205"
     CDS             6877..7572
                     /locus_tag="VLE22_03205"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB13438.1"
                     /translation="MKLRLAIVLLAVAALIAVAAQIQRSGDYRDPDWMYDEGWTTLFN
                     GKDFTGWVPVVAQPDGKAKRFRENEVSQQETFFIKDGKLCTTGKPNGYVRTVDVYDNF
                     VFHVEVRFSESGNSGVLIYVQRDEVWPKGVECQLYQAHMGRIFPIQGATMEGGEMIHA
                     AAKPPGEWNTYEVYSEEGRVATVLNGVLVGIGSNANPRVGYVCLQSEGVPAEFRNIKV
                     KRYTPAWHIRDEK"
     gene            7900..>8664
                     /locus_tag="VLE22_03210"
     CDS             7900..>8664
                     /locus_tag="VLE22_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSB13439.1"
                     /translation="MIEQIRPGVSARHARFVLFDFDGTVSLIRAGWVEVMVPMMVEIL
                     AELKTGESEQQLRAIVEDFVARLTGKQTMYQMIELAEQVEKRGGKPLEPLVYKRMYLD
                     RLNDKIRDRIAELRAGKVSPEKYLVPGARKMIEALAERGLTMFLASGTDQEYMRDEAR
                     LLDIDRYFQGGVYGALDDYKSFSKQILIRKVIDEANCRGEEFLGFGDGYVEIENVKEV
                     GGIAVGVATDEPECRKVDEWKRQRLAGVGADFIIPNF"
BASE COUNT         1728 a         2524 c         2771 g         1641 t
ORIGIN      
        1 aacaataacc caggccgcaa ggggcttgga catggctaga tcttatcacc tcccatccag
       61 acgaaactcc gtccggtaat tcaggttgcg cccatcgctg gacagctcaa ccttcacgat
      121 tttcggatac cgtgcgggca actgtaagtc gagtcgatag tacgagagaa tcgtgcgaaa
      181 catctccttg atggcgccag gaatctcggc gttcgagcgg cagaaggagc tggcgccgcc
      241 ggtggtcgcc gccagttgct tcaggccaac ctggtacgct tcattcagcg tctcgctgcg
      301 atagtcgagg tgcacgacaa aaagaggcgc ttcgaggcgc gcgagtaccc cgtcgagttt
      361 ggagatcttc tcacggatga ggccttcggg aaaccggcgg ctcatgtcgt gcggatcgct
      421 gtaattgatg accggattcg tgtagtcctc ccggtagttg cggatgtcgc tgtcggttac
      481 gtacagcacc gccacccgca cggcagactt ctgcagaatg ccgtcggcga tccgcgcaat
      541 cgtttccacc gtgttgagca tgcccgcatt gccgctgatg gtcatcgcct cgatcgcggc
      601 tgcgagcgcc tcgcgatctg cggtgggatc gaggacgacg ttcagtccgt cttgcgctct
      661 caagagcccc gcgtgcgcag tggcgggaag ttgccgtacg gcttcaatga gtgcctgctt
      721 ggccggagcg atggcggaga cgtcgccgac catgtccgta acgatcagga gcagcagggg
      781 atcgtccgga ccgagaagcg aacggacagt agccgggcgg ccctccagct tcgcgctcac
      841 atttgccgcg gtcagcccgg agtcccctcc ggcggcaacc cacaggggca cgtgaatggt
      901 cttttcgttg gcgaccggcg gggctgcccc gagcaggaga aacggcagaa gaagaccgag
      961 cggccgcacc tagaaactaa gcctcactcc cacctggatg accctgccgc cgcgcgagcc
     1021 gatgatcttg cctgcattca ggttcggcgc actggccggc ccaacgtatt ggtcaggatc
     1081 gttccagttg gcgtggttca ggaagttcaa accgacagcg ctcagctcga tggtgcggtc
     1141 cgcgccgagc acgaagcgct tgttgagggc gagatcgtgg ttctgattgg tcggcgcacg
     1201 caacgtcgga tgtgttcggg cggcgtcgcc aatggtgaag tcggcaggct ggtcgaacgc
     1261 agccggattg aaccacaagt ccggtccttg attcgccacg cttgggtctg cccccggcac
     1321 cacgttcacc cgcagggcgc ggacgactcc accggtgttg ttgaacagcg gccggatcgc
     1381 cagcggctct ccactgacaa ccgaggacga gccgctcagc gaccagcctt cgagggcgag
     1441 ccgcctccag tccgtaagcg agcggccaaa cggcagctcg tagacgtagt tcatgccgag
     1501 atagtgcggc cggttgtacc acgtcaggga ccattcgttg tcgcggttgt aaacatcctg
     1561 gcgtcctgta gggccggagt agtcgtcgta ctgcttcgat acctgatagg tgaggttgag
     1621 agtgagcccg cggctggtgc gcttctccac ccgaagccat ccggcgtcgc ggcggtatcg
     1681 gccgagcggc caggagtcgt tcagctcgaa gcccttgtat tgtggatacg gccgcagcga
     1741 ccggttaaac tcctcgtcgt tgagctggtc gcgatactgg agtgtttcga gccggaggga
     1801 attgggatcg gcggtgtttt cgctcaccgg cagatttttt ccccccgcgt aggccagccc
     1861 ggccgtcagc acaaacgacc cgggcaactc gcgctcgagc gaaagcgacg ccgactggta
     1921 gatcggctgg ttgtcggtcg tgttgattaa gtcggcgatc gtgtcgtttg cggcttcggg
     1981 acgcgtgtcc ggaacggggt ggtcgggcgc aggaaatccg tcggtaagga tgagcgccgg
     2041 ctgaagctgt gtgttcggcg agatgtacgt cggcgtggcg ttgaacgcct gggttccgaa
     2101 ctgaccgtag taaaccggga tgacactgta gtcgcggctg aagttcgcgc ggacgacggt
     2161 tttcgtgtct ccgcgcgggt tccaggctaa ccccaggttc ggctccactt tgactagtgt
     2221 cggctggaaa gcacgcccat ggcctccttg cccagcaacc acgagtgcgc cgggccggtc
     2281 gttcgctggg ttgatcgcgg tcagatcgat cgtggactgg cggtcgtact tttcgaccct
     2341 tggcgtgtaa caggacatgc tcgcgccgcc actgatggta aacccccgcc ttagctcgta
     2401 ggaatcgcgc atcgtcacaa acgtggacga gcggcgaaaa tacgacggcg ccacgacaat
     2461 gctacgcgcg ccgtactcgg cgtaacccag caggaaactg gagaaggcgt gacccgtgtt
     2521 gacgattcca ggcaggcctg tcaattcggg ctcaaaacgg aggaatccgg tcgggtactg
     2581 cggccagtaa gagtggacct ggaatcgcgt gtgccgcgtc acaagccgga ggttgtgctt
     2641 tccccggcgg ctggaaagtg agtccgacca gctaaacgaa tttcgtgtat tggtcgagat
     2701 cggataagac cggcccatcg acaggtaagg gtagatcctc aggtacggaa aggtgggtcc
     2761 ggagactccg ctgagtccca attctgccgg gtagtcggcc tcggtatcgg aaccgttctt
     2821 cgaaccgtcg gtggtcgctt cgaagccgag tgtattcacc gtctgggaag atgacgtgaa
     2881 cacgtgttcg acggaggccc gccgggaatg aaaccggcga tcgttcgcgc ctggattggc
     2941 cggaccaggc agcaattccg ccgccccgag ggaaccattc gagtacgaca gatcgaaatt
     3001 cagacggtgc cgctcgcgga gcgtgtggtc cacctttgca atcattccgt tcgctgtgtt
     3061 ggtctccggc gagacgagga agtaattgtt tcggtcgaac ggacccacgg cggcgttcgg
     3121 cttcggatag taactcagtg ctttctgcgc aacagaatcg agttggtgcg ctgggatgcg
     3181 gttgccggga aactgctcgc gtaagtactc gaggttctcc gtagacaccg cctgcgaggg
     3241 gtcaaagttc ggattgggtc gggtcgatag cggatcgaag attggcagca acacgcccga
     3301 ttgatcgacg gtcgccgaga agtcgccgcc gtgctcgggg gcggtggcaa tggtagtaag
     3361 attcgagcgc gacacacgct cgcgcacgcc ctcgtacgag accgaaaagt gagtggaccg
     3421 cgtgccggag taaaggaagg gaatcactac cgggccgccg atgttgaacc caaactggtt
     3481 ccgccgaagc agagacttat tgccgccgag ctgaacgatc tcatgaggca cggcgtccag
     3541 caaatcgttc cgcaggttct caaacaccct gccgtgccat ttccaacggg cgccgttctg
     3601 ttttcgcttg cgaggactgt cggggcgcag acccatgtcg cgggtcagga ccttaaactc
     3661 ctcgaccgcc ttcgtcatct ccgcgcccgg ctccgcctgc tgcgaaagag ccgcgcaggc
     3721 acaccccaag ctcgtgatga gaagccggaa tgcactcctc atacttatga gtgtaacctg
     3781 tagaggcagg cgtcgatcct gcttccgaag gctatgaaac tcaaagacat cgcggctcgc
     3841 ctggagtgcc gtctgcacgg aaatggcgag atcgaaatca ccggtctggc gggcatggag
     3901 cgcgccggtc cgtcggagct gaccttcctg gccaatcaga agtacatccc gaaagtgaag
     3961 cacacgaagg cggccgccat tctggtcacg gagccggtcg agggcgtgcc gatcgcgagc
     4021 ctcgtgtccc gaaatccgta tttcgacttc gctcgcgcgc tcgaactctt ctatcggccc
     4081 ccgcgtcctg cacccggcat tcacccgctg gcgtacgtgt ccccgagtgc ggagatcggc
     4141 gacggcgcct ctatcgggcc gttcgcggcg gtcggtgacc gggtgaagat cggccgcaat
     4201 gcgatactcc atccccacgc cgtcatctat gagggcgtgg agatcggtga cgacttcacc
     4261 gcacactcgc actcctcggt acgcgagtac tgccggatcg gcaaccgagt aattctccag
     4321 aacggcgtcg ttgtcggcgg cgatggcttc ggcttcgcga agcgcgagga cggcacgcat
     4381 tacaagattg tgcagtccgg tcctacggtc atcgaggacg acgtcgagat ccagtcgctt
     4441 tcgaacatcg accgggcgac ggtcggtgaa acgcgcgtaa agcggggcgc caagatcgat
     4501 agcctggtgc aggtgggcca tgcttgcgtc gttggcgagg acaacatcat ctgcgctcag
     4561 gttggcatgg ccggcagttc catcctcgag cgtaacgtgc tcttggccgg acaggtgggc
     4621 gtctccggcc acctcacggt gcacgataac gcgatcgtat atgcccagag cggggtcggt
     4681 ggcgacgtgg acccgggcgc cgtgatctca ggatccccgg cgttcgatgc cgtggcctgg
     4741 cggcggtcgg tggcggcctt ccagcggttg tcggacctcg tcaggacagt gcgagaatta
     4801 aagaatagag tggcgcaatt ggagagtgcc gccccatcat ccaccgattc ccatgaataa
     4861 ccaaaaccag gcccgctatc gccgtcccaa ggcgtacaag aacgaaaggt ttctcgatag
     4921 cgccgacgca cgccccctgc ggatcctttc ggaatacctc gagccgctct ctcactttcg
     4981 gcgggagaaa atccgggaca cgatcgtgtt tttcggctcg gcgaggctgc atgaggacgg
     5041 cccgatgggg aagtattaca acgaggcgcg ggagttggcg cggctgctga ccgagtggtc
     5101 gaaccagctc gaaaacacca gccggcggtt cgtggtctgc accggcggcg ggccggggat
     5161 catggaggcc gccaaccgtg gggcgcagga cgcgaacgga aagactgttg gcctcaacat
     5221 cggcttgccc ttcgagcagt tgcccaaccc gttcatcacc ccggaacttt ccttcgagtt
     5281 ccactacttc ttcatgcgca aattctggtt cgcctacttg gccaaggctc tggtggtctt
     5341 cccaggcggt ttcggcaccc tggacgagct gacggagttg ctgacgctcg cgcaaactga
     5401 aaagctggcg aagaagatca ttatcgttct ctacggaacg agttactggc gagaaatcct
     5461 caacttcgag gcgctcgtgc gtcacgggat gatctcggaa gaagacctcg acctgtttca
     5521 gttcgccgac gacccccaga ctgcgctgcg gattctccag gatggattgc ggaagtacta
     5581 tctcgaaccg ggcgtgccgt tgcccgagcg cgagatggag acgcccgcga tcgcgaagtc
     5641 ccgaatctga cggcattgga cgaccggaat gaaatactgg tacattcctt tgtttactga
     5701 actaaatcgg cggagaccgc acgacaaccg aaaatcattg agagaggaca aatgagtcaa
     5761 ttcaatcgca ggtatttttt gatgggaagt gtggccgcgt cgatcgcgcg gccgagcatg
     5821 ggccagagcg caaacgacgc tctcaacgtc ggcatgatcg gcaccggaaa ccggggttac
     5881 tacgtgatgg gcggcgtaat gaagcagccc ggcgtgaaag tggtcgcggt gtgcgacatc
     5941 aaaccggatc gcctcgataa agcggccact gccgccagcg gcgcgaagac ctataccgat
     6001 taccacaagc ttctcgctca gaaggacatc gacgctgtgt tcatcgccac accgtgcgac
     6061 ctccacgtcg agatgtcgat tgcggcgctc aaggccggca agcatgttta ctgcgagaag
     6121 ccggtcggga tcacgccgga atcgattcgc gagctcgtga aggcggcgaa ggcgtccaac
     6181 aaggtttttc aggtcggaca gcagatgcgt gccgacgcac ggctgcgcaa gacgatcgag
     6241 cgcatccacg aaggcgtggc cggaaaggtc gtgatggtca aggcgcaacg gcacgccggc
     6301 gacgatctcg accacgatgg ctcgtcggcc gactggttct tcaacgccgc ccgctcgggc
     6361 gacgtgatca ccgagatgtc ggtacacaat ctcgatgtct gcaactgggc gatcgacagc
     6421 cttcccgaac gtgccgccgg cttcggcggg gcgctggtgt ggccaaacat tcccccgggc
     6481 cggaccaaca tggacggcta cgcgctgagc tacgagtacg ccaacggcgt gaaactctcc
     6541 tatacacaag ttttcttcca tccgaacggt atgcctaacg gcggccagta cttcaacgtg
     6601 ttcaccaccg agggcggcgt cgacctgatg agttcgacgt tttactcgcg cgagcggggc
     6661 aagaagcccg tggtgctggt gccaccggag aacactccgg acgatgcaca caccgcggcg
     6721 ttcttcgaag cgattcgcac cggttcgaag ccgtttgccg acatccgcgt gggggccacg
     6781 ggcgcgctga ccgccgtcat ggggcgcgag gcgatctatc ggaagaagat gatgacctgg
     6841 cgcgagatgg gcgtcgacat ctagggaact tcgagcatga agctcagact ggcgattgta
     6901 cttctggccg tggccgcgct catcgcggtc gcggcgcaga tccagcgcag cggcgactac
     6961 cgggatccgg actggatgta cgatgagggc tggacgacgc tattcaacgg aaaggacttc
     7021 accggctggg tgccagtggt tgcgcagccg gacggcaagg cgaagcgttt ccgcgagaac
     7081 gaagtcagcc agcaggaaac gttcttcatc aaggacggaa agctctgcac caccggcaag
     7141 ccgaacgggt acgtgcgcac ggtcgacgtt tacgataact tcgtcttcca cgtcgaggtc
     7201 cggttctcgg agtccggcaa cagcggtgtg ctgatctatg tgcagcgcga cgaggtctgg
     7261 ccgaagggcg tcgagtgcca gctttatcag gcgcacatgg ggcgcatctt cccgattcag
     7321 ggagccacga tggagggcgg cgagatgatt cacgctgccg ccaaaccccc gggggagtgg
     7381 aacacctacg aggtctactc cgaggaaggg cgcgtcgcta ccgtgctgaa cggagtgctg
     7441 gtgggtatcg gctcgaacgc caacccgcgc gttggatacg tctgtctgca atcggaaggc
     7501 gtgccggcgg aattccgcaa tatcaaggtg aagcgctaca ccccggcctg gcacattcgc
     7561 gacgagaagt agactggcac actgcgcttg tctccgcttc atcggtgact tggcgccctg
     7621 aggcattatc gctcctggaa tcaatacaat agtcgcttgc gcagtcatct cgctggaact
     7681 atgccgtgta tgatttgagt gaaaggatcc gccgtgctgg attccgccac gtagttgccc
     7741 cgtttcgagt ggcgcatcgt gatggacgat gcgtccctag gcccgcaacg gatcaatcga
     7801 atcaaagagt taggccgtgt ttgcgttacg cacatatatt ttgagaatgc gtccctgtta
     7861 gccaccgcct ccggcgatcg catggcacaa taggaaagaa tgattgagca gattcggccg
     7921 ggcgtgtcgg ccaggcacgc ccggtttgtc ttatttgact tcgatggtac ggtctccctc
     7981 atccgggcgg ggtgggtgga agtgatggtc ccgatgatgg tcgagatcct cgccgaactc
     8041 aagacgggcg agagcgagca gcaacttcgc gccatcgtag aggactttgt cgcccgcctg
     8101 accggcaagc agacgatgta ccagatgatc gaactggctg aacaggtcga aaagcgcggc
     8161 ggcaagccgc tcgaaccgct ggtctacaaa cggatgtatc tcgaccggct gaacgataag
     8221 atccgagatc gcattgcaga actccgcgca ggcaaggttt cgcccgaaaa gtacctcgtg
     8281 ccgggcgccc ggaagatgat cgaagctctc gccgagcggg gcctgacaat gttcctcgcc
     8341 agcggcaccg accaggaata catgcgggat gaggcgcgat tgctcgacat cgaccgctat
     8401 ttccagggtg gtgtctacgg cgcgctcgac gactacaaga gtttctcgaa gcaaattctt
     8461 atccggaaag tcattgacga ggcgaactgt cgcggcgagg agttcctcgg cttcggcgac
     8521 ggctacgtcg aaatcgagaa cgtcaaggaa gtgggaggca tcgcggtggg tgtcgccacc
     8581 gacgaacccg agtgccgcaa ggtggacgag tggaaacggc aacggcttgc cggtgtcggg
     8641 gcggacttca tcatccccaa cttc
//
LOCUS       DATIFQ010000074        30402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1364316, whole genome shotgun sequence.
ACCESSION   DATIFQ010000074 DATIFQ010000000
VERSION     DATIFQ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 30402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30402
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364316"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2941
                     /locus_tag="VLE22_03215"
     CDS             <1..2941
                     /locus_tag="VLE22_03215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HSB13440.1"
                     /translation="AGIRALIGAEITSVENCRYSLLAENRTGYQSLCRLITKMKLRAK
                     KGEAAATPEDLAEHAAGLICLTGGEEGPLALALRGGPEEGRRCAERLVSIFGKGNVYA
                     EVQRHYDREEEARNQAVAGLGLPLVACNGVSHAREEQRELLDVLTCVRHKTTIHEAGR
                     LLAKNSERHLKTPAAMKRLFAGLPEAIHNAEELAARIEFTLANLGYQFPHYPVPAGET
                     MNSFLRKLTEECARNRYRPYHERARKQIERELAVIEKLDLAGYFLIVWDIVRFCREKG
                     ILAQGRGSAANSAVCYVLGITAVDPVGMELLFERFLSEERGEWPDIDIDLPSGERRER
                     VIQYVYQRYGKLGAAMTANVITYRDRSAARDVGKALGFDPDSLARLSSLVRGFEWDDP
                     EETVERHFREAGFDLNEPLVAKFISLYAQAQDLPRHLGQHSGGMVICQGQLDSIVPLE
                     PATMPGRVVVQWDKEDCADMGVIKVDLLGLGMLAAVEESLVLIRDAYGEEVDLAHLPQ
                     DDPLVYATLQRADTIGMFQVESRAQMSCLPRLRPEKFYDLVVEVAIIRPGPIVGNMVH
                     PYLQRRQGLEPVVYPHPSLEPVLKRTLGVPLFQEQLLRMAMTVAGFTGGEAEELRRAM
                     GFKRSEKRMREIEVKLRQGMDRNGIDKTAQDEIVKSITSFALYGFPESHAASFALIAY
                     ASAYLKCHYLAAFTAALLNNQPMGFYHPATLIKDAQRHGSHFKTADVTQSGRTCTVED
                     DNGKKCVRLGLNYVKGLRAEMAAAIVEQRERASFRSIEDLTARVPLRKDELATLAEVG
                     ALNSLEREGHRRSALWDAALAVRPAGELFAGSGENGSRSPLASMTLPERLLADFRNTG
                     VTIGPHPMRLHRERMRTMGVKSAAELKGLRNGRPVRIGGCVICRQRPGTAKGFLFISL
                     EDETGVANAIVTPDLFTEYRTVIVNAPYLLIDGALQNQEGVIHVRARHIEALPLGAAT
                     VTSRDFC"
     gene            3190..4593
                     /locus_tag="VLE22_03220"
     CDS             3190..4593
                     /locus_tag="VLE22_03220"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HSB13441.1"
                     /translation="MTGNAVWLIASWRLALPAGLLAWAGAQVGGLLPSPAWQAIAGIA
                     PLAGRLTPSANAAPPQAHIAHHEIYCAMTVVWLAGILTCLLRWHRQLRTGGRVHREPN
                     QFERQAFQTAEEGTGLHQRVRLVISAGPTEPVLRGILRPRIILPEGLSGVLSPQELVT
                     VLSHELVHAMRRDNLTAALHRAVACVLWFHPAAWWIERRMAEERGHACDEAVVRRGAV
                     REDYASALLMTCRFCAGCATAGVSGVTGGTNMKERIERIIHAPARPAARRALALVAAV
                     IVAAVWPAVGAFLVQAGESGVKKLLPGIEWVRIMSAGKVVYDSSQAGPAAAPDAFLAR
                     SRTAPSGLTAPYRKWLEEDVTYLITDAERAEFLRLPSDSDRERFIERFWKGRDPSPGT
                     AANEFKEEHYRRIHYANERFRDDGPGWRSTRGRVYVKLGPPDEIESHPRDRYEAWKYA
                     RWGNLPQSLFVKFVLPE"
     gene            complement(4604..5320)
                     /locus_tag="VLE22_03225"
     CDS             complement(4604..5320)
                     /locus_tag="VLE22_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HSB13442.1"
                     /translation="MNADFLVPVSRTTVSIEVCRTLLGYILAGDWKDGDRLPGERELC
                     RRLGVGRASLREALKGLQVLGMIEMRQGEGTFVSSQNGFLTRPLLWAISGTGPDSFSQ
                     LYEARRVLEGELAALAAERATDEELSAIEALVLRMEAEGFTNVVLGEADVEFHLCIAK
                     AARNVVLQNLITLLLHLTRQMTREVMLPPTVSGITAQLHRQLYEAIKRRDAAGARAAC
                     AQHFQTALDRRRRAFAQPGL"
     gene            5508..8801
                     /locus_tag="VLE22_03230"
     CDS             5508..8801
                     /locus_tag="VLE22_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB13443.1"
                     /translation="MKHLVIACLLILAVCGLAFSQTTFATITGTVTDASGAVVPQANV
                     TVTNLETNITTTATSNEAGVYSAAQLREGNYSLKAEAPGFAPFVTQGITLTARDIRRL
                     DVTLQVGATSTAVEVSAGATLIETETARISNTQNDRLMKSMPLNARWLWAYFNQVPNM
                     INGSDGYRFAGSTGNENNWSIDGTTMNDGQGWAIGPQLNYIESFQEVKVDMANNTAEF
                     GAIGHVDVVTKSGTNELHGSVYDTFMTPGLRARNFFSDSIWTGRFHIFGGSIAGPVYI
                     PKVYNGKNKTFFFFSMERSAGNASFTNLNPTVPIAAWRQGDFSGLRDEGGNPITIYDP
                     TTGSPFPNNRIPAGSINNVSKLIQDQYYPLPNFGDPNVFQANNYREIKTMPWSVPISR
                     TLRLDHHFSDKNFVYGRAIITNGPATWWESNLPTVGLHRQTRPTRSATVSYTHVFSAS
                     LINEARWGMDYNNIPSEGPVRGYDEVKRLGLTGLAPNLPDVPGLLQVNWSGIGLQGIS
                     QENGNNPGFRNHNEQWMDHVSWFRGRHTFKFGVDLARVEGDDFSIPGNLFGAVNFSNK
                     FTSLNKAGEVGGHPYADFLLGIPTYSSRAFPSLLLERNRWQYATYFADEFKVTPSLTL
                     NVGLRYQLHQPWRENHGRMSIFDIGSGSIVVPDASIAQISPLFPTSYVNVVSASSAGL
                     PSETLIRPDKNDFAPRVSLAWRPFGNNTVLRAGYGIFYEVTPFDFTSSGSPFQLAEPG
                     FTNPADNPLVILPRVFPDRAIGSPYATVNLPSAVNPGLITPRSQQYNATIEHQQWNTG
                     FRLSYIGTAQRQGSYSYNYNSPIPDDRPFTSKARPFQQYPEIMYRTNGAGHQYHGLSA
                     EVKRSMVKNLYFQAAYTLARDRYDAFRWGTIENPFDRHREVGNAFDMPRHRVTANWVW
                     DLPFGKGRKFGGNVNRWVNLIAGGWVLSGTYAFHSGNFLTPYWSGPDPVGIAYTESDA
                     PWVTLRPDQLRDPNLPSGQRTIERWFDTTAFAAPQKGRFGTAAKGTIRGPRCNVLNAG
                     IQKELIFSERGRVILEMTSRNVLNHPNFNNPDMNISNTGSVGRISWAGGISDDGGPRE
                     ERLGLRIEF"
     gene            8952..10250
                     /locus_tag="VLE22_03235"
     CDS             8952..10250
                     /locus_tag="VLE22_03235"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB13444.1"
                     /translation="MSKHPNHLRRDFLKQAGSTAVLPFTAASQRALGGVSPNERINVG
                     FIGVGGMGSGRLSQFMEHPDVNVAALCDVDQTHLDRAVEEVRKQRSTAPPAFRDFRKL
                     LDMKDVDAVTIATPDHWHAITTVQACRAGKDVFVEKPLCHSIAEGRAMLDAAHRSGRI
                     TQLGTHIHNDYPNYRRVVEVVRSGMLGEIHKAYCWIRSSDKGIGNPPDAPPPKELDYD
                     FWLGPAPKRPYNPNRSHGRFRNFWDYSGGVFIDFWCHITDLAYWALDLKAPTSVAAAG
                     GRYLTDDNGETPSLMEVVYQYPGLVLSWNVHSKGMPGFESRSIGCIFQGAEATLVAGY
                     EWHELYVAGKHVPDFKRPDPTIPDSPGHVREFLDAIKSRKRTTCDLEYAFRLTKGGLL
                     GNLAYRTGRRLYWDDQRERIIDDREAQQMTSRRYRKPWRL"
     gene            10307..10834
                     /locus_tag="VLE22_03240"
     CDS             10307..10834
                     /locus_tag="VLE22_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB13445.1"
                     /translation="MRDYLIVGSSRQASLDVRYQQLLEAYASALRRLCAAYLTDQADR
                     QDLFQEIALALWKALPRYRREASERTWLYRVAHNVALTYSGRRRRKDRLERPLEEMAT
                     EPGVTEGQRHRELLEAVQQLPSFDKQLALLYLEGLTGREMEDVTGISANNVAVRLSRV
                     RQRLAASLRPKEVRR"
     gene            10831..11436
                     /locus_tag="VLE22_03245"
     CDS             10831..11436
                     /locus_tag="VLE22_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13446.1"
                     /translation="MNSHESPGDELQAVWRQNPAGKEDVAMTIRLVQEKGRSFQDVVR
                     EQNRAGYLLALALGPLTALAAWKANAAIFQIGYLILTVTVVAGCVVTWLYHRKERSLA
                     DTGASVREYHRQLLEVYDRRIRFLKSVKFWYAIPLLTGASMVLFPIWTRSGNPWDLWM
                     PVTLVVLVWVWMWHFNDVRNVGQVRRKRQEVEELLAQIEHE"
     gene            complement(11489..12796)
                     /locus_tag="VLE22_03250"
     CDS             complement(11489..12796)
                     /locus_tag="VLE22_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB13447.1"
                     /translation="MDSANRFSRRSLLSGASSLAAAPAILAQRNANDRLGVAMVGVGT
                     RGIYLLERAQECPNTEIRVICDLYDSNLKRAADKCFNKKAQLMKDWEKAVSSPDVDAV
                     FIAAPDFWHATMAVRAAENKKHVYVEKALCRTLPEAKAIRKAVRDNKVVLQLGHHQNS
                     DPYFIKAREIYQSGQLGKVALVRTYIDRTSAWPEWQFYTAYNIQETPSDATPATIDWK
                     RFVANAPEIPFDVQRFFRWRCWWEYGTGIAGDLMSHQWDGVNMIVGMGIPEAVQTMGG
                     LYFWKQDREVPDQWHVMFEYPKKEMNVTFACTFHNRHHGTNTYVFGRDATLEVHTDWC
                     RVYGAEWKPDFPKKWAAARKAAESMGMLPNDAIVAPDYSMKKGELEVTDHMRNFIDCI
                     RSGETPRCGLQRAWEEAVTVVMSVEAFFKERKVKWDPVSEEIV"
     gene            complement(12846..15314)
                     /locus_tag="VLE22_03255"
     CDS             complement(12846..15314)
                     /locus_tag="VLE22_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="interleukin-like EMT inducer domain-containing
                     protein"
                     /protein_id="HSB13448.1"
                     /translation="MPILLILGHIAAAGSLRAGSIRLESGRLWAGDRKFVVKGIAYSD
                     VPIGRRPADEFPAAACLWARDLPLVAATGANTVRTYALLPADAGVFLELLASNDLYWL
                     ADFPLDRYYDPTRRLRESQDRILAEFRAYAERFRGQPRLIGYVFGNEVLSAYPRKFAG
                     PYSDFDDLLAGAAGVLRQLEPEGTPLLTTTARAPAEVHDVAGLSFWSWNAAGLNGFTE
                     TLAAAKGRTHLPVLVSEFGVDAFDAQAGAENEQASADAGMSLADTIESAEGVLGGVYR
                     SFVDEWWRSGPDSSIHSITGSDDSSFPDGVRNDSWFGIFRATESGIADLDSLRPRLAF
                     RALAGRWGGRVPASFDLEDNPRIAKLEHAATGEETAAPGVLLRLSGDALASTESSAAL
                     LDRWPLHLETSCLCLDGRPAPLGRVTPDIVTAQAPWDVKLGDEVAIWFRSGRAANPMM
                     LSIREYAPGIFPDAVVRAGTTCRASAENGVRPGEVLEVYATGLGSGASSDSVEASVNG
                     ASAEVLYAGLVPSFVGLNQVNLRVGAATPPSLSARLVLRIGGISGPPYPLPVAAVTDR
                     YGVSLAYSGGEIVLQAGGEPRTVPVRVEGVNGYCGPVLFTALDPEPGLSFRSPTAFTG
                     QSVPLELRASAAARPAEALAFHLLGSAAGTLGGRVSIPVTVLPSRGEIPVRVISGGFR
                     SKPIARFDWNSRTIFAASGGGPGRGINLMAVNPSTGVFDAVRTFDTWGDEGASSRLVA
                     YLAALPPRTIVLFAVADEATYLLSQQARDTISALFGSRAIGELGYQHSWAMIGRKGDE
                     TPLAERSSGDTQVVLERVLSLPSP"
     gene            15547..17442
                     /locus_tag="VLE22_03260"
     CDS             15547..17442
                     /locus_tag="VLE22_03260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HSB13449.1"
                     /translation="MRRRAAGRSHLVVRFATMPSPEQSRELDRRGVRIVEYLPEGGFA
                     VAVDDGADLDSLNIVAAVRPRASQKISPYLKEDAAPQDETAVVEFFLDVEREDSRALL
                     QEQGFEIVDHPDLVRNQWLVRGEWWRLRELAGWDEVSYVFPGSAELARGERVRGCPGA
                     VTAFGYVGQSAALVGDGWDGPGRGSAQLGYFLEQLSRKLPPDATKQEIQRAMLEWARY
                     ASLTFLPSSEAQSGRTLNILFAPRNHGDAYPFDGFSGVLAHTYYPAPPNPEPRAGDIH
                     FDDDEEWVTGPDLSRYSADLFSVALHEFGHALGLGHSDLPGTVMYPYYRRIAQLAADD
                     IAAIQTLYAAPGTNPGGGGALTLQIQNPGVFPYTTTQSSVSLSGTVTGGSGAVTVSWS
                     TNRGAAGVAQGGREWSISSLPLQTGDNAVTIVAIDEAYTRSSRTVTITLSSGTTAPDL
                     RITLPTTASTYTATSPAVTIAGTASDASGIARVEWVSARGGSGLASGTSNWRTGTIGL
                     QTGSNEIILTATAVSGGRSSRKLTVSFGGGPDTVAPAITVTYPASAAVVTYSASISVR
                     GTATDNTGVSAVTWSVSSGKSGNAVGTNFWATPEIPLLVGTNTVVVRAFDAAGNTSWR
                     SITVTRR"
     gene            complement(17445..19232)
                     /locus_tag="VLE22_03265"
     CDS             complement(17445..19232)
                     /locus_tag="VLE22_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HSB13450.1"
                     /translation="MSRPTLLLATILLWGCSDSGPKPASSVDEFLTMYNELDRRLYTV
                     AAEAQWKAVTDVTEEHTGARIGADRALAAFRGSRYVIDSSRRFLDLRESLNDLKFREL
                     DKILLNAAESPGTIPDVVQARIEAEARLTAAMDGFTFCLERRGEQCLKPITANEIDDI
                     LLNSRNLAERRKVWEASKQIGPPLKAGLAEVRDLRNRTATELGYSSFFHLQVADYGMS
                     VSELMQLMDKVVLETQPLYRQLDLYARRKLAERYGQPVPERLPAHWLPNRWGQEWPGL
                     AGTVDFNGLFKRKEPKWIVEQAERFYTSLGFPALPRQFWEKSDLYQLPPDATRRKNTH
                     ASAWHIDRNQDVRSLMSVVPNFRWFETSHHELGHVYYYLAYSNPKVPIVLREGANRAF
                     HEAVGDLIGAAARQELYLRQLGLLPASQTIDRTQWLLNEALDSAVVFLPWSAGVMTHF
                     EHDLYERKLPAEQFNRRWWELVTKYQGFVPPSERGEQFCDACTKSHLIDDPAQYFDYA
                     VATLLKYQLHDYISRRILKQDPRRCNYYGNKEVGRWLWEILSLGATRDWRQVLREKTG
                     EDLSSRAMIEYFRPLSEYLEKEDASAGKN"
     gene            complement(19283..21955)
                     /locus_tag="VLE22_03270"
     CDS             complement(19283..21955)
                     /locus_tag="VLE22_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSB13451.1"
                     /translation="MTPGVTLGHYRVISRLGSGGMGEVYLAQDTRLERTVAIKILPAD
                     VASDPDRMRRFEQEAKAAAALSHPNVAHIYEIGEEDGVRFLAMEYVEGQTLAELIRNG
                     PFDAPAILDISIQVADALEEAHSRGVTHRDVKPANIMLTSRSRAKVLDFGLAKVRLPK
                     AHAITASAPTQTLAFPATDSGTVVGTVQYMSPEQALGRNVDHRSDIFSLGAVMYEMAA
                     GSPAFPGATAAAVYDGILNRTPVSPLQTNPALPEELERIIYKALEKECELRYQTVADL
                     RVDLKRVRRDIESGTKSAARAAATARRPWTESLLLVSAVLAVLVGVAIFFVQQRHAGK
                     PEAALQANPLTSYPGSESQPSFSPDGNQVAFAWNGETETDWDIYVKVVEAGTPLRLTS
                     NPAADYSPVWSPDGRHIAFLRQSDSGSAFYLIPALGGLEQKLSDASSDRSGLDTPYLA
                     WSPDGRTLALADKDSPQEPSSIFLMAVESGRRRKLTNPERKSPGDSSPVFSPDGKRLA
                     FVRTASLAVQDIYVMSLEGGGQPTRLTFDNRRVMGLVWNPVDGKLIFSSSRAGNSRLW
                     RISPRGGEPERLPGGIGENASFLAISRKGDRLAYTRSVVDTNIWRYPMPGKAAPGETP
                     VRLISSTRHEQGPKYSPDGQRIVFASNRSGSQEIWVSDRNGVNSNQLTSFDGPPTGSP
                     YWSPDGQFIAFDSRPDGNPDIYIVGAGGGVPRRLTSDSSQDIVPSWSRDGRWVYFASN
                     RTGTFEVWKVSPGGGPAVQVTRAGGFHATESPDGRYIYYTRAPGAPGLWRLPVDGGDE
                     EPVIEGLRAGFWSYWAIVNEGVYFLDRQEVPKEGIHYYLRFFELKTRKDRLITELPKR
                     PFNSGLDISPDGRSFLYTQVDQSDTDIMLVDNFR"
     gene            complement(22031..22558)
                     /locus_tag="VLE22_03275"
     CDS             complement(22031..22558)
                     /locus_tag="VLE22_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13452.1"
                     /translation="MAGGSLASSIHGVVRATQDIDLVVKLRPPQVDQLVAELGPAFYA
                     DAGVIRSALAAGRPFNLIHYATGYKFDLFPLKTDAYYELQFHRRVIEHPVVFDGEFEL
                     PVATAEDTILAKLAWYRAGGEVSERQWNDVRGVILVQRNRLDVDYMKRWAKHLEVEDL
                     LDRVLRECGGELSAE"
     gene            complement(22621..22893)
                     /locus_tag="VLE22_03280"
     CDS             complement(22621..22893)
                     /locus_tag="VLE22_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13453.1"
                     /translation="MTVPASEWYADTDPRALGVFLGVQREMPVSRKIETVLQMSEMIR
                     RLSEANVRRLHPDASDREVFLRTVARRLDRETMICVYGWDPIAAEP"
     gene            complement(22890..24194)
                     /locus_tag="VLE22_03285"
     CDS             complement(22890..24194)
                     /locus_tag="VLE22_03285"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HSB13454.1"
                     /translation="MSNIVVLGAQWGDEGKGKIVDLFSERFDVVARYQGGHNAGHTVR
                     IGDKKFILKLIPSGILRPGKQAVIGNGLVIDPAALIDEIEALETLGIDVRSQLAVSNR
                     AHVIFPFHRMVEKIAESRPDRVAIGTTSRGIGPCYEDKVGRRGIRVGEFIDPESFACL
                     YGYLAEDKQVLARAFGIDDPIDFGAIRQQYTQLAERIRPMVCDTAALLNDAIGSGKRV
                     LFEGAQGTMLDIDHGTYPFVTSSSAAAGGVCTGAGVPPTKIHGVLGVSKAYITRVGGG
                     PFPTEAKDEMGERIRKVGNEYGAVTGRPRRCGWFDVPLLRYTAMVNGFDSLVITKLDV
                     LDELDDIPVCTAYRVDGRESLEMPATRRGVEAIEPVFERLPGWRARTAGVTRYEDLPV
                     RARDYLWFLQDHTGVEIGCISTGPERDQTIVMPGSKLEKLLG"
     gene            24251..24712
                     /locus_tag="VLE22_03290"
     CDS             24251..24712
                     /locus_tag="VLE22_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13455.1"
                     /translation="MRLSEVFVQLGEQRFGDLVRRISIGKLRTYQLYESFKTRAHLGK
                     VNTETLRKAAPRLWARVQENDEDLAKDTAQAILLTHFDMVKSVLDFLGIPNEDGFFAK
                     DLDASSYLTDGWQQRVYDKFHGAYPEPAILLYVNHLAWELEKEPQLFKPAS"
     gene            24743..25765
                     /gene="pheS"
                     /locus_tag="VLE22_03295"
     CDS             24743..25765
                     /gene="pheS"
                     /locus_tag="VLE22_03295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HSB13456.1"
                     /translation="MLDATLSQLETRARECIAAAATAQDLEPVRIEVLGRKGALAQVS
                     KDLGKLPPDERARIGKLLNAVKQSLEEAMEARKAQFDAAVLAARLDAEWLDLTLPAPE
                     PERGHLHLVSQIQAELEDIFTSLGFTVLDGPEVETEYHNFDALNIPQDHPARDMQDTF
                     WLTDGSLLRTHTSPVQVRGMERLGPPLRMIAPGRVFRNEEVDASHEHTFYQLEGMMID
                     REVSIAHLVYFMKTMLSAIFRREVTVRLRPGYFPFVEPGFELDIRCLLCDGQGCRVCK
                     QSGWLEQVGCGLVHPNVLRNSGIDPEQWMGWAFGVGLTRLVMMRHAIDDIRYLQSGDL
                     RFLRQF"
     gene            25781..28111
                     /gene="pheT"
                     /locus_tag="VLE22_03300"
     CDS             25781..28111
                     /gene="pheT"
                     /locus_tag="VLE22_03300"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSB13457.1"
                     /translation="MKFSYNWLRELVDELETPAAELGRLITMKTAECEGVESVGAHMA
                     NVRAAKVLTAEPLGAGLNKKVVLDAGRFGKKTVVCGAPNCRPGITSAWLPPGTRLGGI
                     EIGTITIDGVTSEGMLASGAELGINRNGAGILEIDVAPGDPIPGCAPDQIIEIDNKSI
                     THRPDLWGHHGLAREVAAITGSRLLDPVKPGLVPGNPAPIGVDIRDLDLCPRYSALAF
                     ENVTVQPSPLWLQYRLEAIGLNPINNIVDVTNYVMAELAQPMHAFDWDTLKGPAIIVR
                     PAVQGEHFVALNQEEYHLDARDLVIADARGAIALAGVIGGLDTSINERTTRLVLESAN
                     FQASSIRKTSSRQKLRTDASMRFEKAQDPLNTVRGLARAVELLCEVSPGIRLVGGLAD
                     QQKETPSPAPIRLPMDWLIRKLGRGVETEEVTEILRRLQFGVSEESPRVLSVSVPSWR
                     ATRDISIKDDLVEEVGRMVGYGSVTPQPPALPVIPPPRNEERLFHRGVRHAMAEQGFT
                     EVYNYSFISEEQALALGMNPEEHVRVANPISAEQTLMRRSLLPEILKNILENSRHFEK
                     FRLFEIGREIHKQPEGLPKETPHLAAVVYAKDDGQAGLMELKRAAGHLMPDCEIRPGP
                     ARGYEHPARAAEVVWRDSALGRLFEFHSSLVSGRAAVLDIDLELMRSLTREDKRYVPI
                     RRFPSSAFDLSVIVGLRDLVGDIRKRLVGFAGADLDQIEFLRQYSGPPLPEGGKSVSF
                     RLTVAAPDRTLSSEEVGVIRGRIIDGMRGLGYDLRL"
     gene            28108..28290
                     /locus_tag="VLE22_03305"
     CDS             28108..28290
                     /locus_tag="VLE22_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSB13458.1"
                     /translation="MKIVLILLFGATALSLPAAELAAPPERVQQWRDYKFGMFIHWEA
                     YSLSSEVNAREILPLY"
     gene            28517..29005
                     /locus_tag="VLE22_03310"
     CDS             28517..29005
                     /locus_tag="VLE22_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13459.1"
                     /translation="MPLTVVDFAPQPELPYAESDVVVVGNITHLQPFLSADKKNIYTE
                     YTLQVVEHVKNAVGVPTVPGDSLTLLRQGGVARFSNGRVVKHEIRNGVEPVPNQQYLV
                     FLRYRPQLDAFVYRKIWLIREGVMKAAYPDDVSREHAGKSKYSGKPLQEVLVTLRDKV
                     TR"
     gene            29243..30166
                     /locus_tag="VLE22_03315"
     CDS             29243..30166
                     /locus_tag="VLE22_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSB13460.1"
                     /translation="MSRFVFRAPLVVLIAAAAIPASGDDQSNNAQRLPTVTVRGASFS
                     LIRIPSGEFLMGSEKEFENPRHRVRIRAFDLGSTEVTVRQFRAFAEATGYRTDAEREG
                     SAWTCHWSVDDAVIWRSRGFWNIEKGASWRNPGFSQSDEDPVVAVSWHDAVEFCRWLS
                     RESGDRFRLPTEAEWEYAARAGTKAEQPSDLGEAAWYKVNSDGTTHPAGRKKPNPWGL
                     YDMLGNAWEWVEDVWSKNYDGAPNDGTPRVDGGSGFASLAPGEVRPLRGGGWCLESAE
                     ARYSARPGFGLHQRCNNSGFRVARELPPPRS"
BASE COUNT         5875 a         9473 c         9450 g         5604 t
ORIGIN      
        1 ggcaggcatc cgcgcgctga tcggagcgga gatcacgtcg gtggaaaact gccggtactc
       61 gctgctcgct gaaaaccgca ccggctacca gagcctgtgc cgtctgatca cgaagatgaa
      121 actgcgggcg aagaagggcg aagcggcggc cactccggag gatctggccg agcacgccgc
      181 cggactgatc tgcctcaccg gcggggagga agggccactg gcactggcgc tgcgcggcgg
      241 tcccgaagag ggccgtcggt gcgcggagcg gctggtgtca atcttcggaa aaggcaatgt
      301 ctacgccgag gtccagcggc attacgaccg ggaagaggaa gcgcgcaacc aagcggtggc
      361 cggcctgggg ctgccgctcg tcgcctgcaa cggcgtcagt cacgcgcgcg aggagcagcg
      421 cgagttgctc gacgtgctca cctgcgtacg ccacaagacc acgatccacg aagccggccg
      481 gctgctggcc aagaactccg agcgtcacct gaaaacgccc gcggcaatga aacgactgtt
      541 cgccggcctg ccggaggcga tccacaacgc ggaagaactg gcggcccgga tcgaattcac
      601 actggcaaac ctcggctacc agtttccgca ttaccccgtg ccggccggtg aaacgatgaa
      661 ttcatttctg cgcaaactga cggaggagtg tgcgcggaac cgctaccgcc cgtaccacga
      721 gcgcgcgcgg aagcagatcg aacgggaact ggcggtgatc gagaagctcg acttggccgg
      781 ctactttctg atcgtctggg acatcgtccg cttctgccgc gagaagggga tcctggcgca
      841 gggacggggc tccgccgcca acagcgccgt gtgctacgtg ttgggaatca cggctgtcga
      901 tcctgtaggg atggaactgc tgttcgagcg gttcctctcc gaggagcgcg gcgagtggcc
      961 ggacatcgac attgatctgc cgagcggcga gcgccgcgag cgcgtgatcc agtacgttta
     1021 ccagcggtac gggaagctgg gcgcggcgat gaccgccaat gtgatcacct accgcgaccg
     1081 gtcggcggca cgggatgtcg gcaaggcgct cggtttcgat ccggactcgc tggcccggct
     1141 gtcgagcctg gtgcgcgggt ttgaatggga cgacccggag gagacagtgg agcggcactt
     1201 ccgcgaggcc ggctttgacc tgaacgaacc gctggtggcg aagttcatat cgctgtatgc
     1261 gcaggcgcag gacctgccgc gtcacctcgg gcagcactcc ggcggcatgg tgatctgcca
     1321 gggacaactg gattcgatcg tgccgctgga gccggccacg atgccgggcc gcgtggtggt
     1381 ccagtgggac aaagaagact gcgccgacat gggcgtcatc aaggtggacc tgctcggact
     1441 gggcatgctg gcggcagtcg aggaatcgct ggtgctgatc cgcgatgcgt acggcgaaga
     1501 ggtggatctg gcacacctgc cgcaggacga tccgctcgtc tacgccacgt tgcaaagggc
     1561 ggataccatc ggcatgttcc aggtggagag ccgcgcgcag atgtcgtgcc tcccgcgcct
     1621 tcggccggag aagttctacg acctggtggt tgaggtggcg atcatccgtc ccggacccat
     1681 cgtcggcaac atggttcacc cgtaccttca gcggcggcag gggctggaac cggtcgtcta
     1741 tccgcacccg tcactcgagc cggtgctgaa gaggacactc ggcgtaccgc ttttccagga
     1801 gcagttgctg cgcatggcga tgacggtggc cggattcaca ggcggcgagg ccgaggagtt
     1861 acggcgcgcg atgggattca agcgctccga aaaacggatg cgtgagatcg aggtgaaact
     1921 gcggcaggga atggatcgca acggcatcga caagacagct caggacgaga tcgtgaagtc
     1981 gatcacgtcg ttcgcgctgt acggttttcc ggagtcgcac gcggccagct ttgcactgat
     2041 cgcttatgcc agcgcttacc tgaagtgtca ctacctggct gcgttcacgg cagcgctgtt
     2101 gaacaaccaa ccgatggggt tctaccaccc cgccacgctg atcaaggacg cgcagcggca
     2161 cggctcgcac ttcaaaacgg cagacgtgac gcaatcggga cgtacgtgca cggtagagga
     2221 cgacaacggc aaaaagtgcg tgcggctggg actgaactac gtaaagggcc ttcgcgccga
     2281 gatggcggcg gcgatcgtgg agcagcgaga acgggcgtcc tttcggagca tcgaggacct
     2341 gaccgcgcgc gtgccgctgc ggaaagatga actggcgact ctggccgagg tgggtgcgtt
     2401 gaactcttta gagcgggagg gtcaccgccg gtccgccctc tgggacgccg ctctggcagt
     2461 gcggccggca ggcgagttgt tcgccgggtc cggagagaac ggttcgcgct cgccgcttgc
     2521 ttccatgact ttgcccgaac ggctgctggc cgatttccgt aacacggggg taaccatcgg
     2581 cccgcacccg atgcgactgc accgcgagcg gatgaggaca atgggcgtga agagcgccgc
     2641 cgagcttaag ggcctgcgaa acggacggcc ggtgcggatc ggtgggtgcg tgatctgccg
     2701 gcagcgtccc gggacggcga aaggctttct cttcatcagc cttgaggacg agaccggagt
     2761 tgcgaacgcg atcgtcacgc ccgatctctt caccgaatac cggacggtga tcgtgaacgc
     2821 gccgtatctg ttgatcgacg gcgcccttca gaaccaggaa ggcgtcatcc acgtccgtgc
     2881 ccgccacatc gaagcgttac ccctcggagc ggcgacggtg acgtcgcggg atttctgttg
     2941 aagcgagcag gcagaagcga ccctctcgga tcagggcaac tcgccgcgat tcacggcaga
     3001 cgctggtaca ccaacacgtc cgcggcttcc gtgttcaggc ggtccgcgtg gcggttgatg
     3061 atttcagcgt ccgcagcgtc acggcgggct ttctcgaggc gtgccagata gaaccggacc
     3121 gctctttcta caaaggcaga gcgatctgca ttcgcccggt caattctctc cagaaggtcc
     3181 aaagggagtg tgactggcaa cgcagtctgg ctgatcgcat cctggcggct agcccttccg
     3241 gccgggttgc tcgcatgggc cggggcccag gtgggcgggt tgcttccctc ccccgcgtgg
     3301 caggcgatcg ccggaattgc accgctcgcc gggcgcctga cgccatccgc gaacgccgcg
     3361 ccaccacagg cccacatcgc tcaccacgag atctactgcg ccatgacggt ggtatggctg
     3421 gcggggatcc ttacgtgcct gcttcgatgg caccggcagc tccggaccgg cggcagggtg
     3481 caccgagagc cgaatcaatt cgagcggcag gctttccaaa ccgctgagga aggcacgggg
     3541 cttcaccaac gtgtccgcct tgtgatctcg gcaggcccga cggagcccgt gctccgggga
     3601 atcctgcgcc cgcggatcat cctgcctgag ggcctctccg gcgtcctgag ccctcaggaa
     3661 ctcgtgaccg tgctttcgca cgagctggtc cacgcgatgc gtcgtgacaa tctaacggcc
     3721 gccctgcatc gcgccgttgc atgcgtgttg tggttccacc cggccgcgtg gtggatcgag
     3781 cgccgcatgg ccgaggagcg ggggcatgcg tgcgatgaag ctgtggtccg gcgcggcgcc
     3841 gtgcgggagg attacgcctc ggcgctgctc atgacttgcc gattctgtgc gggttgcgca
     3901 accgcgggtg tctcgggagt gaccggtgga acaaacatga aagaacgaat cgagcggatc
     3961 atccacgcac cggcgcggcc ggcggcacgg cgcgcgctgg cgctggttgc ggcagtcatt
     4021 gtggcggcgg tgtggcccgc ggttggagcg ttccttgttc aggcgggcga gtcgggcgtc
     4081 aagaagctac tgcctggcat cgagtgggta cgaatcatga gcgcgggaaa ggtggtctac
     4141 gattcgtccc aggccggccc cgcggccgcg ccggatgcgt ttctggcgcg ttcgcggacc
     4201 gcgccctcgg gtctcacggc cccttatcga aaatggctcg aggaggacgt tacctatctg
     4261 atcaccgatg ccgagagggc cgagtttttg agactcccct cggattcgga ccgggagcga
     4321 ttcatcgagc ggttctggaa ggggcgcgat ccgagtcccg gcacggctgc gaacgagttt
     4381 aaggaagagc actatcggcg catccattat gccaacgagc gattccggga cgacggtccg
     4441 ggctggcgaa gcacccgcgg ccgcgtgtac gtcaaactcg gaccgcccga tgagatcgaa
     4501 tctcaccccc gcgatcgtta cgaggcttgg aagtacgccc gttggggcaa tcttccgcag
     4561 tcgctttttg taaagtttgt cctgccggaa taggcggcgg gagttacaag ccgggctgcg
     4621 cgaaggcgcg gcgtcgtcta tccagtgcgg tctggaaatg ctgcgcgcat gccgctctgg
     4681 cgccggcggc gtctcgccgc ttgatcgcct cgtagagttg ccggtggagt tgcgcggtga
     4741 ttccggacac cgtgggaggc aacatgacct cgcgagtcat ctgccgcgtc aggtggagga
     4801 ggagcgtgat gagattctga aggacgacgt tgcgggccgc tttcgcgatg cacagatgga
     4861 actcgacgtc cgcttcccca aggacaacat tcgtgaaacc ctcggcttcc atccggagaa
     4921 cgagtgcctc gatcgccgac agttcctcgt ctgttgcccg ttcagccgca agcgcagcca
     4981 gttcaccctc aaggacgcgc ctcgcctcat agagttggct gaaactgtcc ggacccgtac
     5041 ccgaaatcgc ccatagcagc ggccgggtga gaaagccatt ttggctggaa acgaaggttc
     5101 cctctccctg ccgcatctcg atcattccga gaacctggag acctttcagc gcttcgcgca
     5161 gggaggcccg gcctacaccg aggcgccggc acaactcgcg ctcgcccggt aaccggtcac
     5221 catctttcca atcacccgcc agaatatagc cgagcagcgt tcggcacact tcgatactga
     5281 ccgttgttcg ggacaccgga accagaaaat cggcgttcat cgcattcccg tcaagacact
     5341 ctagcacagg aataaacaag tttcaagtct actggtatgg acatcagtcc agactagagt
     5401 tcgtttctgt aaataactga tattaatgct taaaaaaact tcttgacagt cgtattgccg
     5461 ggcagtaaaa tctattctga ctactggtcc taccagttgg aggttatgtg aagcacctag
     5521 tcatcgcatg tctactcatt ctcgctgtct gcggtttggc tttctcgcag actaccttcg
     5581 caaccatcac ggggacggtc acggacgcct cgggcgcggt tgtgccccag gcgaacgtca
     5641 ctgtcaccaa cctcgaaaca aacatcacca ccaccgcaac ctcgaatgag gcaggtgttt
     5701 attcggctgc ccagttgcgc gagggcaact actcgttgaa ggccgaagcc cccggcttcg
     5761 cgcccttcgt cacacagggc atcaccctga ccgcacgaga catccgccgc ttggacgtca
     5821 cactacaggt gggcgccacg agcacggccg tggaagtctc ggccggggcc acactgatcg
     5881 aaaccgagac cgcccgaatc tccaatacac agaacgacag actgatgaag agcatgcctc
     5941 tcaacgcccg ctggttgtgg gcgtatttca atcaggtgcc gaacatgatc aacggcagcg
     6001 acggctaccg cttcgcaggc agcaccggca acgagaacaa ctggtccatt gatggaacca
     6061 ccatgaacga tggacagggc tgggcgatcg gcccgcagtt gaactacatc gagtcgttcc
     6121 aggaagtcaa ggtcgatatg gccaacaaca ccgccgagtt cggcgcaatc ggccacgtcg
     6181 acgtcgtcac caagtcgggc actaatgaac tgcacggcag cgtgtacgac acgttcatga
     6241 cgcccgggct ccgagcccgc aatttcttct cagatagcat ttggaccggc cggttccaca
     6301 tttttggcgg cagtatcgcc ggaccagtct acatcccgaa ggtttacaac ggcaagaaca
     6361 agacgttctt cttcttttcc atggagcggt ccgccggaaa cgctagcttc accaatctca
     6421 acccgaccgt tccaattgca gcctggcggc agggcgactt ttccggtctg cgggacgaag
     6481 gcggcaaccc catcacgatt tacgacccaa cgaccggctc gccgttcccg aataacagga
     6541 ttccagcggg cagtatcaac aacgtcagca aactgatcca ggaccagtac tacccgctcc
     6601 ccaacttcgg cgacccaaac gtgttccagg caaacaacta ccgcgagatc aagacgatgc
     6661 cctggagcgt cccaatcagc cgaactctgc gtctcgatca ccacttctcc gacaaaaact
     6721 tcgtgtatgg ccgtgcgatt atcaccaacg gaccggcgac ctggtgggaa tccaacctgc
     6781 cgacggtcgg cctgcaccgc cagacgcggc cgacgcgatc ggcaaccgtc tcttacaccc
     6841 acgtcttcag cgcatcgttg attaacgagg cgcgctgggg catggactac aacaacatcc
     6901 cctccgaggg tccggtgcga ggctacgacg aggtaaagcg gctgggcctg accggcttgg
     6961 ccccgaacct tccggacgtc cccggcttgc tccaggtgaa ctggtccggc atcgggctgc
     7021 aagggatctc ccaggagaac ggaaacaacc ctggtttccg aaatcacaac gagcagtgga
     7081 tggaccacgt gagctggttc cgcggccggc acacgttcaa gttcggcgtg gatcttgccc
     7141 gggtcgaggg tgacgatttc tccatccccg gaaacctgtt tggtgccgtg aacttctcga
     7201 acaagttcac gagcctcaac aaggcaggtg aggttggcgg ccatccctac gccgacttcc
     7261 tgctgggcat cccgacgtac tcctcccgtg cctttccgtc attgctgctc gaaaggaacc
     7321 gctggcagta cgccacctac ttcgccgacg agttcaaggt cacgccgagt ctgaccctga
     7381 acgtcggact gcgctaccaa ctgcaccagc cctggcgcga gaaccacggc cggatgtcga
     7441 tcttcgacat tggctcgggc agcatcgtcg tccctgacgc ctcgattgca cagatcagcc
     7501 cactgttccc tacgagttac gtaaacgttg tgagcgcgtc cagcgccggg ctgccgtcgg
     7561 agaccttgat ccggcccgac aagaacgact tcgctccccg ggtcagtctg gcctggcgcc
     7621 cgttcggaaa caacacggtt ttgcgcgcgg gctacggcat cttctacgag gtgacgccgt
     7681 ttgatttcac ctcttccggc agtccgttcc agcttgcgga accgggcttc acgaacccgg
     7741 ccgacaatcc gcttgtgatt ctcccgcgcg tattcccgga tcgcgctatc gggtcgcctt
     7801 acgccaccgt gaatcttccc agtgccgtca accctggact gatcacgcct cggagccagc
     7861 agtacaacgc gaccatcgag caccagcagt ggaacaccgg cttccgcctg tcttacatcg
     7921 gcacggcgca gcgccaggga agctactcgt ataactacaa ctccccgatt ccggacgacc
     7981 gcccgttcac ctccaaggca cggccgttcc aacagtaccc cgagatcatg tatcgaacca
     8041 acggagcggg tcaccagtac cacggcctga gcgccgaagt gaaacgatcg atggtcaaga
     8101 acctctattt ccaggccgcc tataccttgg ctcgtgaccg ctacgacgcc ttccgctggg
     8161 gcacgatcga gaacccgttc gaccggcacc gtgaagtcgg caacgcattc gatatgccgc
     8221 ggcatcgggt gacggcgaac tgggtgtggg atcttccctt tgggaaggga aggaagtttg
     8281 gaggaaacgt caatcgctgg gtcaatctga ttgccggcgg ctgggtcctt tccgggactt
     8341 atgctttcca ctccggaaac ttcctcactc cgtactggag cggaccggat cctgtgggca
     8401 ttgcgtacac ggaaagcgac gccccgtggg ttacgctgcg ccccgatcaa ctccgggatc
     8461 cgaaccttcc atccggccag cgcaccatcg agcgctggtt tgacacaacc gcatttgccg
     8521 caccccaaaa gggccgtttc ggcacagccg ccaagggtac catccggggg ccgcgctgca
     8581 acgttctgaa tgcgggcatt cagaaggagt tgatcttcag tgaacgcggc cgtgtgatcc
     8641 tggagatgac ctcacgcaac gtgctcaacc acccgaactt caacaaccca gatatgaaca
     8701 tctccaacac cggcagcgtc ggccggataa gttgggccgg cggcatctct gatgacggcg
     8761 ggccacgcga agagcgcctc ggtctccgca tcgaattcta gactcagtcg aattgacgta
     8821 caaccccaag ggcgggcctt cgggctcgcc tctttttttg tccatcattc cctcccgctc
     8881 acgccttcct gtctcccgtt cttacggtct cggagcgagt ggtatagaat gttgccgggc
     8941 tcagagaact aatgtccaaa catccgaatc atttgagacg agattttctg aagcaggcgg
     9001 gctccacggc cgtgctgcca ttcaccgccg catcgcagcg ggcgctcggc ggtgtaagcc
     9061 cgaatgaacg aatcaacgtc ggattcatag gtgtcggcgg catgggcagt ggccgcctga
     9121 gccagttcat ggaacatcct gacgtgaacg tcgccgccct ctgcgacgtc gatcagacgc
     9181 atcttgaccg ggcggtcgag gaggtccgca aacagcgctc caccgcacct ccagctttcc
     9241 gcgacttccg caagttgctt gatatgaagg acgtcgacgc ggtgacgatc gccacacccg
     9301 atcactggca cgcgatcact acagtgcaag cctgccgggc cggcaaggac gtgttcgtcg
     9361 agaagccgtt gtgccactcc atcgcggagg gccgcgccat gctcgacgcg gcgcaccgga
     9421 gcggccgcat cacgcaactc ggcacgcaca ttcacaacga ctacccgaat taccgccgcg
     9481 tcgttgaggt ggtccgctcg gggatgctgg gcgaaatcca caaagcgtac tgttggatcc
     9541 gctccagcga caagggcatc ggcaatcccc ccgatgcgcc tccgccaaaa gagctcgact
     9601 atgatttctg gctcggcccg gccccgaaac gcccgtacaa tccgaaccgc tcgcacggac
     9661 ggtttcgaaa cttctgggac tactccggcg gcgtgttcat cgacttctgg tgccacatca
     9721 cagatctcgc ctactgggcg ctcgatctga aagctccgac gagcgttgcg gcggcaggag
     9781 gacgatatct cacggacgac aacggtgaga cgccgagttt gatggaggtt gtgtatcagt
     9841 accccggatt ggttctttcc tggaacgtac actcgaaggg gatgccaggc ttcgagtcga
     9901 ggtcgatcgg ctgcatcttt cagggcgccg aagcgacgct ggtcgcgggg tacgaatggc
     9961 atgagttgta cgtcgcggga aagcacgttc cggacttcaa gcggcccgac ccgacaattc
    10021 ccgactcgcc cggacacgtc cgtgaattcc tggatgcgat caagtcccgc aaaagaacca
    10081 cctgcgacct ggagtacgcg tttcgtctga cgaagggagg cctgctcggg aacctcgcct
    10141 accggaccgg caggcggctc tattgggacg atcagcggga gcggatcatc gacgatcggg
    10201 aggcacagca gatgacctcg cgccggtacc gcaagccctg gcggctgtag acagcgcctc
    10261 ggtagagctc ggcggacggc aacatcacct gtaagttttt cggccggtga gggactatct
    10321 gattgtgggc agttctcggc aggcaagcct cgatgtccga tatcagcaac tgttggaggc
    10381 ctacgcgtcc gcactgcgca ggctctgtgc ggcatacctg accgaccaag cggaccggca
    10441 ggaccttttc caggagatcg ccctcgctct gtggaaggct ctgccccggt atcgccgtga
    10501 ggcgagcgag cgtacctggc tgtaccgcgt cgcgcacaac gtggctctga cctattccgg
    10561 aaggcgccgg cgtaaagaca ggctggaaag gccgctcgag gagatggcca ccgagccggg
    10621 tgtaacggaa gggcaacgtc acagggagtt gctggaggcg gtacagcaac tgccttcctt
    10681 cgacaagcaa ctcgcgcttc tctacctcga aggacttaca ggccgggaga tggaggacgt
    10741 cactggcatc tccgccaaca acgtcgcagt gcgtctctca cgcgtgcgac aacggctggc
    10801 tgcctctttg cgcccaaagg aggtgagacg atgaactccc acgaatcgcc aggcgatgaa
    10861 ttgcaggcgg tctggcggca gaatcctgcg ggaaaggaag acgtggcaat gacgatccgg
    10921 ctggttcagg agaaaggcag atcctttcaa gacgtcgttc gcgagcagaa tcgggcgggg
    10981 tacctgctgg ccctggccct cgggcccctg accgctcttg cggcgtggaa agcgaatgcg
    11041 gcgatcttcc aaatcgggta tctgattctg acggtgacag tcgtcgccgg ttgcgtggtt
    11101 acctggctct accatcgcaa ggagcggtcc ctcgcagaca ccggggcaag cgtgcgggag
    11161 tatcaccggc aattgctcga agtatacgac cgccggattc gtttcctgaa gagcgtcaag
    11221 ttctggtacg ccattcctct cctgacgggc gcatcgatgg tcctcttccc gatctggact
    11281 cgaagcggta atccgtggga tctgtggatg cctgtcactc tggtggttct ggtgtgggtg
    11341 tggatgtggc acttcaacga cgttcggaac gtgggacaag tgcgacgcaa gagacaagag
    11401 gtcgaggaac tgctcgcaca aatcgaacac gagtgaggct taccgcggaa gttctgacgt
    11461 ctatgcgcga gggggcgcgc cgcgccccct aaacgatctc ctcgctcacg ggatcccact
    11521 tcaccttgcg ttccttgaag aaggcctcga ccgacatcac caccgtgacc gcctcctccc
    11581 aggcgcgttg aagcccgcac cgcggcgtct cgccggagcg gatgcagtcg atgaagttgc
    11641 gcatgtggtc cgtgacttcc agctcgccct tcttcatcga gtagtccggt gcgacgattg
    11701 cgtcgttggg cagcatgccc atcgattcgg cggctttgcg agcggcggcc catttcttcg
    11761 ggaagtcggg cttccactcg gcgccgtaga cacggcacca gtcggtatgt acctcgagcg
    11821 tcgcgtcgcg gccgaagacg taggtattcg tcccgtgatg gcggttgtgg aacgtgcagg
    11881 cgaaggtgac gttcatttcc tttttcggat actcgaacat cacgtgccac tggtccggca
    11941 cctctcggtc ctgcttccag aagtacagcc cgcccatcgt ttggacagcc tccggaattc
    12001 ccataccgac gatcatgttg acgccgtccc attggtggct catgaggtcg ccggcgatcc
    12061 cggtaccgta ctcccaccag cagcgccatc ggaagaaccg ctgaacgtcg aaggggatct
    12121 cgggagcgtt ggcgacgaag cgcttccagt ctatcgtcgc cggcgtcgcg tcagacgggg
    12181 tctcctgaat gttgtacgcc gtgtagaact gccactccgg ccatgcactc gtgcggtcga
    12241 tatacgttcg caccagcgcg actttgccga gctggccgga ctgatagatc tcgcgcgcct
    12301 tgatgaaata cggatcgctg ttctggtgat ggcccaactg caggaccacc ttgttgtccc
    12361 tgaccgcctt gcggatcgcc ttcgcctcgg gcagagtgcg gcacaaggct ttctctacat
    12421 agacatgctt cttgttctcc gccgcgcgaa cggccatcgt cgcatgccag aaatcgggcg
    12481 cggcgatgaa gacggcatcc acgtcgggcg aggaaaccgc cttctcccag tccttcatga
    12541 gctgggcctt tttgttgaaa cacttgtcgg cggcgcgctt gaggttggag tcatagagat
    12601 cgcagatgac gcgaatctcc gtgttcgggc actcctgagc gcgctcgaga agatagatgc
    12661 cgcgagtgcc gacgcccacc atcgccactc caaggcgatc gttggcgttg cgctgcgcga
    12721 gaatcgccgg ggccgccgcg agtgaagagg cgccggagag aagacttctg cgggaaaaac
    12781 ggttggctga gtccatatca atcactcaag gggatatttc agcacaatcc ggcccacttc
    12841 agaaatcacg gcgacggaag cgacagtacg cgctccagga ccacctgcgt gtcacccgag
    12901 gaacgttcgg ccagaggagt ttcgtcgccc ttccggccga tcattgccca tgagtgttga
    12961 tagccgagct cgccgatcgc gcggctgccg aacagagcag agatggtgtc gcgggcctgc
    13021 tggctcagca ggtacgtggc ttcgtccgcc acggcaaaca gtacgatcgt tcggggcggc
    13081 agagcggcca ggtatgcgac gaggcggctg gaagctccct cgtctcccca cgtgtcaaac
    13141 gttcgcacag cgtcgaagac cccggtcgag gggttcactg ccatcaagtt gatgccgcgg
    13201 cccggccccc caccgctggc ggcaaagatc gtacggctat tccagtcgaa gcgtgcgatc
    13261 ggtttcgacc gaaagccccc ggagatcaca cggactggga tctcgccgcg gctgggtaat
    13321 accgtaaccg ggatgctgac acggccgccg agcgtaccgg ctgcgctccc tagcaaatgg
    13381 aacgcgagcg cctcggccgg cctggcggcg gccgaggcac gcagttcaag tgggacactc
    13441 tgcccggtaa acgcggttgg cgagcggaac gagaggcccg gctctggatc cagagccgtg
    13501 aagagcacgg gaccgcagta gccgttgacg ccttccacgc gcaccggtac cgtccgcggc
    13561 tcgccgccgg cctgaaggac gatctcacca ccggagtagg cgagcgagac gccgtagcgg
    13621 tcggtgaccg cggccaccgg caaggggtaa ggcgggccgg agatgcctcc gattcgcagg
    13681 acgagacgcg ccgacaggga gggaggggtg gcggcgccga cccgcagatt cacctggttc
    13741 aatccgacaa acgacggaac caggcccgca tagagtacct cggcggacgc accattaacc
    13801 gaagcttcga cgctatcgga gctggctccg gaccccaggc cggtcgcata tacctcgagt
    13861 acctcgcccg gccggacgcc attttccgct gacgcccggc aagtcgtgcc ggcgcgaacg
    13921 acggcatccg ggaagattcc aggagcgtac tcacgaatcg aaagcatcat cgggttcgcc
    13981 gcccggccgg aacggaacca tatggcgacc tcgtcgccca gcttgacgtc ccagggagcc
    14041 tgtgcggtca cgatgtccgg tgtaacccga cccaacgggg ccggtcttcc gtcaagacaa
    14101 aggcacgacg tttccaaatg cagcggccac cggtccaaga gagcagcgga cgattcggtg
    14161 gaggcgagcg cgtcccccga caggcgcagc agaacgccag gcgccgcagt ttcctcgccc
    14221 gtggcggcgt gttcgagctt cgcaattctg gggttgtctt cgagatcgaa cgacgccgga
    14281 acccgtccgc cccagcggcc ggcaagagct cgaaacgcga ggcggggacg cagactgtct
    14341 aggtccgcaa tgccggattc agtggcacgg aagatcccga accatgaatc gttcctcaca
    14401 ccgtcgggaa agctgctgtc gtcactccca gtaatcgagt gaatcgagga atccggcccg
    14461 gagcgccacc actcgtcaac gaagctcctg tagacacccc caaggacgcc ttcggccgat
    14521 tcaatcgtgt ccgcgagcga catccccgca tccgccgacg cttgctcgtt ctcggcgccg
    14581 gcctgcgcat cgaaggcgtc gacgccgaac tcggagacga gaaccggaag gtgtgtgcgg
    14641 cccttggccg cggcgagcgt ttcggtgaaa ccgttaaggc cggcggcgtt ccagctccag
    14701 aacgagaggc cggctacgtc gtggacttcg gcaggcgcgc gggcggtggt ggtcagaaga
    14761 ggagttcctt cgggctccag ttgccggagc acgccggctg cccccgcgag gaggtcgtcg
    14821 aaatcggagt agggacccgc gaacttacgt ggatacgctg acaggacttc gttgccgaag
    14881 acgtatccga tcaaccgggg ctggccccgg aaccgctctg cgtaagcccg aaactccgcc
    14941 aggatccggt cctggctttc gcggaggcgc cgagtgggat cgtagtagcg gtccagaggg
    15001 aaatcagcca gccagtagag atcattggac gcgagaagct cgagaaacac gccagcgtcg
    15061 gctggaagaa gtgcgtaggt acgaaccgta ttggctccgg tggccgccac aagcggcaga
    15121 tccctcgccc agaggcaggc ggcggcaggg aactcatcag cgggccggcg tccgatggga
    15181 acgtctgagt aagcgattcc cttaacgacg aactttcggt ccccggccca aagtctgccg
    15241 ctttccaggc ggatcgagcc tgcccggaga gaaccggcag cggcgatatg ccccaggatc
    15301 aacagaatcg gcatcgttag gcggaaatac tggcagcgca tacgacagat tccttactga
    15361 actgagtggc cgccgtatgg gagttgtctc agatcgttcg ataggtcaga tgtgaagtgt
    15421 catagggtct ttttgcccct tcttctcgtg gggatggccg tcacagcggc agacagcccc
    15481 ctggttctgc gcaaaccgag gcgcctggaa gcaaccgccg aaccgcgggc cgccctggat
    15541 tacccagtga gacgaagggc ggccggccgc tcgcaccttg tggttcggtt cgcgaccatg
    15601 ccgtcgccgg aacagagccg ggagctggac cgacggggtg ttcggatcgt cgaatacctg
    15661 ccggagggcg ggtttgccgt cgctgttgac gatggagcgg acctcgacag cctcaacatc
    15721 gtggcggccg tgcgcccacg cgcgagtcaa aagatcagcc cgtacctcaa ggaggacgct
    15781 gctccgcaag acgagacggc cgtagtcgag ttcttcctgg acgtcgagcg cgaggattca
    15841 cgggcgctgc tccaagaaca gggattcgag atcgttgacc atccggatct cgtccgcaac
    15901 cagtggctcg ttcgcggcga gtggtggcgt ctgcgagaac tggccggatg ggatgaggtt
    15961 tcctatgtct tccctggctc cgctgaactt gcccgcgggg agcgagtccg cgggtgtccc
    16021 ggggcggtga cggcgtttgg ctatgtgggg caatcggcgg cgttagtggg cgacggttgg
    16081 gacgggccgg gacgcggtag cgcgcaactt gggtacttct tagagcaact gtcgcgcaag
    16141 ctcccgccag acgcgacgaa gcaggaaatc cagcgggcca tgctcgagtg ggccaggtac
    16201 gcaagtctta ctttcctgcc gtccagtgaa gcgcaaagtg ggcgtacgct gaatatcctc
    16261 ttcgctcccc gaaaccacgg tgatgcgtat ccgttcgacg gctttagcgg ggtgcttgct
    16321 cacacgtact accccgcccc gccgaatccc gagccgcgtg ccggagacat tcatttcgac
    16381 gacgacgagg agtgggtgac cgggccggac ctttccaggt attcggccga tcttttctct
    16441 gttgccttgc acgagtttgg tcacgcgctc ggactcggcc actcggatct accggggacg
    16501 gtgatgtatc cgtattaccg ccggatcgca caactggcgg cggacgatat cgccgcgatt
    16561 cagacgctgt acgccgcgcc cgggaccaat ccgggcggcg ggggcgcact cacactccag
    16621 attcagaacc ctggcgtctt tccgtacacc accacccaaa gttcggtgag cctctccggc
    16681 acggtcacgg gaggaagcgg ggcagtcacg gtgagctggt cgacgaaccg gggagccgcg
    16741 ggtgttgcgc agggcgggcg agagtggtcg atctcctccc tgcctctcca gaccggggac
    16801 aatgcggtga cgatcgttgc gatcgacgag gcgtacacca gaagctcacg cacggtcacg
    16861 atcacgctga gcagcggcac gacagcgccc gatcttcgga ttaccttgcc gacaacagcg
    16921 agcacataca ccgcgacttc accggccgtg acgattgccg gtaccgcctc ggatgcctcc
    16981 ggaattgcgc gtgtcgagtg ggtcagcgcg cgcggcggaa gcggcctggc ttcggggacc
    17041 tccaactggc ggaccggaac gatcgggttg cagaccgggt ccaacgagat catcctcacc
    17101 gcgacggcgg taagcggcgg caggtcttcc cgcaagctga cagtgagctt tggcggcggg
    17161 cctgacactg tcgcgcccgc catcaccgtc acttacccgg cttcggccgc cgtagtgacg
    17221 tattcggcct cgatttcggt gcgcggcacg gcgacggaca acaccggtgt cagtgccgtt
    17281 acgtggtctg tctcgagcgg gaagtcgggc aatgcggtgg gaacgaactt ctgggctacc
    17341 cctgaaatcc cgctgctggt tggtacgaac acggtcgtgg tgagggcgtt cgacgccgcc
    17401 ggaaacacgt cctggagatc gatcactgtg acgcggaggt aggctcagtt cttccccgcg
    17461 ctggcgtctt ctttttccag gtattccgac agcgggcgga agtactcaat catcgcgcgc
    17521 gagctgaggt cctcgccggt cttctctcgc agcacctgcc gccagtcgcg cgtggcgccg
    17581 aggctcagga tttcccagag ccagcggccc acctccttat tcccgtagta attgcagcgg
    17641 cgaggatcct gcttgaggat gcgccgggaa atgtagtcgt gcaactgata cttcagcagg
    17701 gtggcgacgg catagtcgaa gtactgagcg gggtcgtcaa tcaggtggct cttcgtgcag
    17761 gcgtcacaga actgctctcc acgttcggag ggcggtacga atccctggta tttggtcacc
    17821 agttcccacc agcggcgatt gaattgttcc gccggcagct tgcgttcgta caggtcgtgt
    17881 tcgaagtgag tcatcacccc tgccgaccaa ggcagaaaaa caaccgcact gtctagcgcc
    17941 tcgtttagca gccactgcgt ccggtcgatc gtctggctgg ccggcagcaa ccccaactgg
    18001 cggaggtaca actcctggcg ggcggccgct ccgatcaggt ctccaacagc ctcgtgaaat
    18061 gcacggttcg cgccctcacg gaggacgatg ggcaccttgg gattggaata cgcaaggtag
    18121 tagtagacgt ggcccagttc gtgatggctc gtctcgaacc accggaagtt cggcacgaca
    18181 ctcatcaggc tgcggacgtc ctggttgcgg tcgatgtgcc aggccgaggc gtgcgtattt
    18241 ttgcgccggg tggcgtcggg tggtagctga tagagatcgg acttttccca aaactggcgc
    18301 ggaagcgccg ggaagccaag tgacgtgtag aagcgctccg cctgctcgac gatccattta
    18361 ggttcttttc gcttgaacag gccgttgaag tcgacagtcc cggccagccc cggccattcc
    18421 tgtccccaac ggttcggtag ccagtgcgcc gggaggcgtt cgggaacggg ctgcccatat
    18481 cgctcggcga gtttgcgccg cgcgtagaga tccaactgac ggtacaacgg ctgggtctcc
    18541 aggacgacct tgtccatcaa ttgcatcagt tcggagacgg acatgccgta atcggccacc
    18601 tgcagatgga agaacgacga gtagccgagc tcggtggcgg tccggttcct gagatcacgg
    18661 acctcggcca aaccggcctt tagcggcgga ccgatctgct tggacgcctc ccacaccttc
    18721 ctccgctcgg caagattgcg ggaattcagc aaaatgtcgt cgatctcgtt ggccgtgatc
    18781 ggcttcaggc actgctctcc gcgccgttcc aggcagaacg tgaacccgtc catcgccgcc
    18841 gtcagtcgcg cctcggcctc aattcgcgcc tgcacgacgt cgggaatggt ccctggcgac
    18901 tcggcggcat tgagaaggat tttgtccagt tcccggaact tcaggtcgtt gagagactct
    18961 cgcagatcga ggaagcggcg gctgctgtcg atgacgtagc ggctccctcg gaatgccgcc
    19021 agcgcacggt ccgcgccgat gcgtgctccc gtgtgttcct cagtgacgtc agtaaccgct
    19081 ttccattggg cttcggcggc aaccgtgtac agacgccggt cgagttcgtt gtacatcgtc
    19141 aagaactcgt caacactcga ggcgggcttt ggtccggaat ctgagcagcc ccatagcagg
    19201 atcgttgcca gcaggagggt gggtcgactc acacggcaat tgtagaacga aagcggcgga
    19261 atcaaagtgt cccgttttcg ggtcaccgga agttgtcgac cagcatgata tcggtgtcgc
    19321 tctggtccac ctgggtataa agaaaggagc ggccgtccgg cgagatgtcc aggccggagt
    19381 tgaacggccg tttggggagt tcggtgatga gccggtcctt gcgcgtcttg agttcgaaaa
    19441 atctcaggta gtagtgaatt ccttctttcg gcacttcctg ccggtcgagg aaatagacgc
    19501 cttcattaac aatggcccag tagctccaga agccggcccg aagcccttcg ataaccggct
    19561 cctcgtcccc gccgtcgacc ggcagccgcc agagacccgg tgctccgggg gcccgtgtgt
    19621 agtaaatgta cctgccgtcc ggcgactcgg tggcatggaa accaccagcc cgcgtgactt
    19681 ggacggccgg accacctccg ggagaaacct tccacacctc aaacgtcccg gtgcggttgg
    19741 aggcgaagta gacccaccgc ccgtctcggg accagcttgg cacgatgtcc tgcgaactgt
    19801 cggaggtgag cctccgcggg acgcccccgc cggcgccaac gatatagatg tccgggttgc
    19861 cgtccggccg cgaatcgaac gcgatgaact ggccgtccgg cgaccagtaa gggctgccgg
    19921 tagggggccc gtcgaaagaa gtcaactgat tcgaatttac cccgttgcgg tcgctaaccc
    19981 aaatctcctg gcttcccgag cggttcgacg cgaacacaat gcgctgccca tcgggggagt
    20041 acttcggtcc ctgctcgtgg cgggtcgacg agatcaaccg gacgggcgtc tcccctggag
    20101 cagctttgcc cggcatcggg taccgccaga tatttgtatc gaccaccgaa cgtgtgtagg
    20161 cgaggcggtc acccttgcgc gagatggcaa ggaacgaagc gttttcaccg ataccgcccg
    20221 gaagccgctc gggttcaccg cctctgggtg agatgcgcca cagccgggaa ttgccagcgc
    20281 gcgaagacga gaagatgagc tttccatcga cggggttcca gaccagcccc atgacgcgtc
    20341 gattgtcgaa cgtaagcctc gtcggttgac cgcctccttc caacgacatg acgtagatgt
    20401 cctggaccgc caaactggcc gtcctcacaa aagccagccg ctttccatcc ggggagaata
    20461 ccgggctgct gtcaccgggc gacttcctct cagggttcgt gagtttccgg cgacgcccgc
    20521 tttcgaccgc catgaggaag atgctcgacg gctcctgcgg cgagtccttg tctgctaagg
    20581 ccagtgtcct gccgtccggc gaccaggcga ggtaaggagt atccaacccg ctgcgatccg
    20641 aactggcatc ggagagcttc tgctcgagtc cgccaagagc cggaatcaga tagaacgccg
    20701 agcccgagtc cgattggcgc agaaaggcaa tgtggcgtcc atccggggac cacaccgggc
    20761 tgtaatcggc ggcaggattg cttgtgaggc gaagcggcgt gccggcttcc acaactttca
    20821 cgtaaatgtc ccagtccgtc tcggtctccc cgttccacgc aaaggcgacc tggttgccgt
    20881 caggtgagaa gctcggctgg ctctcgcttc ccggatagga cgtgagcgga tttgcctgca
    20941 gagcggcctc cggcttgccg gcatgtcgct gctgaacaaa gaaaatcgcc acgcccacca
    21001 ggacagcgag cacggcggaa accaacagca gagactcggt ccatggccgc cgtgcagtcg
    21061 cggcggctcg agccgcgctc ttcgtgcccg actcgatgtc ccggcgcacc cgcttgaggt
    21121 ccacgcgcag gtcggcgacg gtctgatatc gcaactcgca ctctttttcg agagccttgt
    21181 aaatgatccg ttcgagttcc tccggtagtg ccggattggt ctggagaggc gataccggcg
    21241 tccggttgag aatcccgtcg tacaccgccg cggctgtcgc gcccggaaac gccggagacc
    21301 cggcagccat ctcgtacatc acggcaccaa ggctgaagat atccgaacgg tggtccacat
    21361 tgcggccgag ggcctgttcg ggactcatgt actggaccgt accgacaacg gttccggagt
    21421 cggtggccgg gaatgcaagg gtctgcgtcg gcgcggatgc ggtgatcgcg tgagccttgg
    21481 gcaggcgcac cttggctaga ccgaaatcca ggactttcgc gcgggaacgc gacgtcagca
    21541 tgatgttcgc cggcttcacg tcacgatgag taactccccg ggaatgggct tcttcgagcg
    21601 catccgcgac ctggatcgag atatcgagga tggccggcgc atcaaacggt ccgttccgga
    21661 tcagctcagc gagggtctgt ccttcgacgt actccatcgc cagaaaccgg acgccgtcct
    21721 cttcgccgat ctcgtagata tgggcaacgt tcgggtggct gagcgccgcg gctgctttcg
    21781 cctcctgctc gaaacgccgc atcctgtcag gatcggaagc cacgtcagca ggcaagatct
    21841 tgatcgccac ggtccgctca aggcgcgtat cctgggcgag ataaacctct cccatgcctc
    21901 cggatccgag acgcgaaatc acccgatagt gccctagcgt caccccagga gtcatggtcg
    21961 tcgatctttc gaatgtagca cagggggatc gccggacagc accggaaaac gcctgaaaaa
    22021 gttctgtggg ctattccgcg gacagttctc cgccgcactc tcgaagaacg cgatcgagca
    22081 gatcctccac ttcgaggtgc ttcgcccaac gcttcatgta gtccacgtcc aaacggtttc
    22141 gctgcacgag aatcacaccg cgcacgtcat tccactgtcg ttcggacacc tctccaccgg
    22201 ctcgatacca cgcaagcttc gcgaggatcg tatcttccgc agtggctacg ggcaactcga
    22261 attccccgtc gaagacgacc gggtgctcga tgactcggcg gtggaactgc aattcgtagt
    22321 aggcgtcggt tttcaggggg aagaggtcaa acttgtatcc cgttgcgtag tgaatcagat
    22381 tgaacggcct gcccgctgcg agagcactcc ggatcactcc cgcatctgca tagaacgccg
    22441 gcccgagttc cgcaacaagc tgatcaacct ggggtggccg cagcttcaca accaggtcga
    22501 tgtcctgggt cgctcggact accccgtgga tcgaacttgc cagcgaacct ccggccatga
    22561 atggaatccc tatccggttc aaagcgtcta tgaccaatct taatggtgtg tccggtgatg
    22621 tcatggctct gcggcgatgg ggtcccagcc gtaaacgcag atcatcgtct cacggtccaa
    22681 ccgccgggca accgtccgca ggaagacctc gcggtcgctg gcgtcggggt ggagcctgcg
    22741 cacattcgcc tcagacaaac gccggatcat ttcggacatt tgaagcaccg tttcgatctt
    22801 tcggctgaca ggcatctctc gctgcacacc gaggaacacc ccaagagccc tcggatcggt
    22861 gtcggcgtac cattccgaag cgggcacggt caccccagca gcttttcgag cttcgatccc
    22921 ggcatcacga tcgtctgatc gcgctcggga ccggtcgaaa tgcagccaat ctcgacgccc
    22981 gtatggtcct ggaggaacca caggtagtcc cttgcccgca caggcaggtc ttcatagcgt
    23041 gtgacgccgg ctgttcgggc acgccagccg ggcagccttt cgaacaccgg ctcgatcgct
    23101 tccacacccc ggcgggtggc cggcatctcg agcgattccc tgccgtcgac gcgatacgcc
    23161 gtgcaaacgg ggatgtcatc gagctcgtcg agcacatcga gcttggtgat caccaggctg
    23221 tcgaagccgt tcaccatcgc cgtgtaccgg agaagcggca cgtcaaacca cccacagcgc
    23281 cggggacggc cggtaaccgc gccgtactcg ttgccgacct ttcggatacg ctcgcccatc
    23341 tcgtccttgg cttcggtcgg gaacggaccg ccgccaaccc gcgtgatata agccttcgag
    23401 acgccgagga cgccgtggat cttcgtgggc ggcacaccgg ctccggtaca aacgccgccg
    23461 gcggccgcgc tcgacgaggt gacgaacgga tacgtgccat gatcgatatc gagcatcgtg
    23521 ccctgggcgc cctcgaacag tacccgcttg cccgagccga tggcgtcatt gagcagcgcc
    23581 gcagtgtcgc agaccatggg ccggatgcgc tctgcgagtt gcgtgtactg ctgccggatc
    23641 gcgccaaagt caatcggatc gtcgataccg aaggcgcggg cgagtacctg cttgtcctcg
    23701 gccagatagc cgtacagaca ggcaaaactc tccggatcga tgaattcgcc gacgcggatg
    23761 ccacggcggc cgactttgtc ctcgtagcac gggccgatac cgcgcgacgt cgtgccgatg
    23821 gccacgcggt ccggccggct ctcggcgatc ttctccacca tgcggtggaa cgggaagatc
    23881 acgtgagccc ggttgctgac ggcgagctgg ctcctcacgt cgatgcccaa ggtttcaagc
    23941 gcttcaatct cgtcgatcag cgccgcggga tcgatgacca gaccgttccc gatgactgcc
    24001 tgttttcccg ggcgcagaat gccgctggga atcagcttga gaataaattt cttgtccccg
    24061 atacggacgg tgtgcccggc gttgtggccg ccctggtacc ttgccacaac gtcaaaccgt
    24121 tcggaaaaaa gatcgacaat cttccctttt ccttcgtcgc cccactgtgc gcccaaaacg
    24181 actatgttgc tcatgttgaa gtgcggaacc aacttattgt agcgcacgcg gctgtgctac
    24241 cctgctttcg atgcggcttt cggaagtgtt tgtccaactc ggcgagcagc gctttggcga
    24301 ccttgtccgg cggatctcga ttggaaaact aagaacttac cagctctatg agtcctttaa
    24361 gaccagggca cacctgggga aagtgaatac ggaaacgctg cgtaaggcgg ctccgcgtct
    24421 gtgggcgcgc gtccaggaaa acgacgaaga tctggcgaag gatacggccc aggcgatcct
    24481 tttgacccac ttcgacatgg tgaaaagcgt gctcgatttc cttggcatcc cgaacgagga
    24541 tggtttcttc gccaaggacc tcgacgcctc gtcttacctc acggatggct ggcagcagag
    24601 ggtctacgac aagttccacg gcgcgtaccc cgaaccggcg atcctgctct acgtcaacca
    24661 cctcgcttgg gaactggaaa aggaacctca gctcttcaag cccgcgagct gatctcacgt
    24721 tagaataaga agtttcccaa ccatgttgga tgccacgctg tcacaactgg agactcgtgc
    24781 acgcgaatgc atcgcggcgg cggccactgc gcaggatctc gaaccggtgc gcatcgaagt
    24841 gctcggacgg aaaggcgcgc ttgcgcaggt aagcaaggac ctcggaaagc tccctcccga
    24901 cgagcgggcg cgtatcggca agctgctgaa cgccgttaaa caaagtctcg aagaggccat
    24961 ggaggcgcgc aaggcgcagt tcgacgccgc tgtcctcgcc gctcgcctcg acgccgagtg
    25021 gctcgacctg acccttcctg cgccggaacc ggaacgggga catctgcacc tcgtgtcgca
    25081 gattcaggcc gagctcgagg atatcttcac ttcgctcggc tttacagtgc tggacggtcc
    25141 ggaagtcgag accgagtacc acaacttcga cgccctgaac atcccgcagg accatccggc
    25201 tcgcgacatg caggacactt tctggctgac agacggcagc ctgctgcgaa cgcacacttc
    25261 accggtgcag gtgcgcggca tggagcgcct cggaccgcca ctccggatga tcgctcccgg
    25321 ccgcgtgttc cgcaatgagg aagtcgacgc ctcgcacgag cacaccttct atcaactgga
    25381 aggaatgatg atcgaccgcg aggtctccat cgcgcacctg gtctacttca tgaagacgat
    25441 gttgagcgca atcttccggc gcgaggtgac cgtacggttg cggccgggtt attttccctt
    25501 tgtcgaaccg ggcttcgagc tagatatccg atgcctgctc tgcgatggac agggctgccg
    25561 cgtctgcaag cagagtggat ggctcgaaca ggtcggttgt ggcctggtgc atcccaatgt
    25621 gctccgaaac agcggcatcg atcccgagca gtggatgggc tgggcgttcg gcgtgggttt
    25681 gacgcggctc gtgatgatgc gccatgccat cgacgacatt cgctatctcc agagcggcga
    25741 tctcagattc ctgaggcaat tctaagagga gcccccgacc gtgaaattct cctacaactg
    25801 gctccgtgaa ctcgtagacg agctggagac gccggctgcc gagctcggcc gtttgatcac
    25861 tatgaaaacg gcggagtgcg agggtgtcga gagcgtcggc gcgcacatgg cgaacgtgcg
    25921 cgcagcaaag gtcctcacgg cagaaccgct aggcgccggc ctcaacaaga aagtcgtctt
    25981 ggatgccggc cgcttcggca aaaagacggt cgtgtgcggc gcgcccaact gccgccccgg
    26041 tatcaccagc gcctggctgc ccccgggcac gcgacttggc gggatcgaga tcggaacgat
    26101 caccatcgac ggcgtgacca gcgagggaat gctcgccagc ggcgcggagc tcggcatcaa
    26161 ccgcaatggc gccggtatcc tcgagatcga cgtcgcgccg ggcgatccta tccccggttg
    26221 tgcgccggac cagatcatcg agatcgacaa taagtcgatc actcaccgcc cggacctgtg
    26281 ggggcatcac gggctcgccc gcgaggttgc ggcgatcacg ggatcacggc tcctcgatcc
    26341 ggtgaagcct ggccttgttc ccggcaaccc ggcgcccatt ggcgtggaca ttcgcgacct
    26401 cgacctctgc ccgcgttaca gcgcactggc cttcgaaaac gtcaccgtcc aaccctcgcc
    26461 gctgtggctc caataccggc tggaagcgat cggcctcaac ccgatcaaca acatagtcga
    26521 cgtgacgaac tacgtcatgg ccgagctggc ccagccgatg cacgccttcg actgggacac
    26581 gctaaagggc ccggcgatca tcgtacgtcc ggccgtccag ggcgagcact tcgtcgcgct
    26641 gaaccaggaa gagtatcacc tggatgcgcg cgacctcgtg atcgccgacg cgcgcggagc
    26701 aattgcgctg gcgggcgtca tcggcggcct cgacacgagc atcaatgagc gcaccacacg
    26761 gctcgtgctc gaaagcgcga acttccaggc ttcctccatc cgcaagacgt cgtcccggca
    26821 gaagctgcgg acagacgcct cgatgcggtt cgaaaaggcg caggaccccc tgaatacagt
    26881 aaggggactg gcccgcgcgg ttgaactgtt atgcgaagtc tcgccgggaa ttcggctggt
    26941 gggcgggctc gccgaccagc aaaaagaaac gccgtccccc gccccgatcc gcctgcccat
    27001 ggactggctg atccgcaagc tcggccgcgg cgtggaaacc gaagaagtga cggagatact
    27061 gaggcgcttg cagttcggcg tgagcgagga atcgccgcgg gtgctttcgg tcagcgtgcc
    27121 gagctggcgc gccacccgcg atatctcgat caaggatgac ctggtggaag aggtcggccg
    27181 gatggtgggc tacggctcgg tgacgccgca accccccgcg cttccggtga tcccgccgcc
    27241 tcgcaatgaa gagcgattgt tccaccgagg cgtgcgccac gccatggcgg aacaaggctt
    27301 caccgaggtc tacaactact ccttcatcag cgaggagcag gcgctggctc tcgggatgaa
    27361 tcccgaggag cacgttcggg tcgccaaccc gatttcggcc gagcagacgc tcatgcggcg
    27421 cagcctgctt ccggagattc tcaagaacat tctcgaaaat agcaggcact tcgagaagtt
    27481 ccggctcttt gaaatcgggc gagaaatcca caagcagccg gaggggcttc cgaaagagac
    27541 gccgcacctc gcggccgtcg tctacgccaa agacgatggg caggccggct tgatggaact
    27601 gaagcgcgcc gccgggcacc tgatgccgga ctgcgagatc cgtccgggac cggcccgcgg
    27661 ctacgaacac ccggcgcgcg ccgccgaagt cgtctggcgc gacagcgccc tcgggcgcct
    27721 gttcgagttt cattcgtcgc tcgtcagtgg acgcgccgcg gttctcgaca tcgacctcga
    27781 actgatgcgg agtctgaccc gtgaggacaa gcggtacgtt ccgatccggc gcttcccgtc
    27841 cagtgcgttc gatctgtccg tgatcgtcgg actgcgcgac ctcgtcggcg acattcgaaa
    27901 gcgcctggtc ggtttcgcgg gcgcggacct cgaccagatt gagttcctgc gccagtattc
    27961 cggcccgccg ctgccggagg gaggcaagag cgtctcgttc cgcctgaccg tagccgcgcc
    28021 cgaccgcacc ctatcctccg aagaggtggg tgtaatccgt ggacgcatca tcgacggcat
    28081 gcgcggtttg gggtacgatc tccggctgtg aagatcgttc ttattcttct tttcggcgcc
    28141 accgcacttt cccttcccgc cgccgaactg gccgctccgc ctgagcgcgt ccagcagtgg
    28201 cgggactaca agttcggcat gttcatccac tgggaggctt actcgctctc cagcgaggtg
    28261 aatgcccgcg agattctccc gctatactaa aacctgttct cggtcgaggc aggtgactat
    28321 gcaattttgt agctccttcg ctgtccttct aacttttgcc ggcttcgttc tgacacaggg
    28381 ctccagccaa caagttccct ttttcctgtc ccccgcagag gacaccgtac cgcaatgggt
    28441 gcgcgatgca cggaacaacg cctacaacgt gcgacccgga cgaccatcga tgatagaccc
    28501 cgaggagtca ggcctattgc ctctgaccgt cgtggacttc gcgcctcaac cagagttacc
    28561 atacgccgaa agtgatgtag tagtggttgg caacatcaca catctgcagc cctttcttag
    28621 cgcggacaag aagaatatct ataccgaata cacgctgcaa gtggtagaac atgtgaaaaa
    28681 tgcagtcggg gttccaacag tacccggcga ttctctaacc cttctgcgcc agggtggggt
    28741 ggctagattc tccaacggac gtgttgtcaa acacgaaatc cgcaacggcg tcgagccggt
    28801 tcctaaccag cagtacctgg tttttctccg gtatcggcct cagcttgacg ctttcgtcta
    28861 tagaaaaata tggcttatcc gggaaggtgt gatgaaggcg gcatatcctg atgatgtgtc
    28921 acgcgaacac gcaggcaagt ccaaatactc ggggaagcct ctacaggaag tactagtcac
    28981 cctcagagat aaagtaaccc gataggcgac gcgctctgca ccgcagtcca aaagactgcc
    29041 atgcatgtca cttgcggtga ctggtctgcc gcagtctcag gtcagctcgg cacgtccttt
    29101 gaccgtgggt ttcaactttc ggatggtgcg tcattcggtc gggggatgcg gacgcaggct
    29161 gacgaatcac ggcgaaacgc gtccagtcta caatatttgg atctgttacc ctgatttcga
    29221 attacaatcg agaggggcac ttatgtctcg attcgttttc cgggcgccgc tcgttgttct
    29281 gatcgcagcc gcggcaattc ccgcgtctgg tgacgaccag tcaaataatg ctcaacggct
    29341 ccccactgtt acggtccgcg gcgcgagctt cagcctgatt cgaatcccgt cgggagagtt
    29401 tctaatggga tcggagaagg aatttgaaaa cccgcgccat cgagttcgga tcagggcttt
    29461 tgacctcggc tcgaccgagg taaccgtccg ccaattccgc gctttcgccg aggcgactgg
    29521 ataccgcacc gatgccgagc gagaagggtc cgcctggact tgccattggt cggtcgatga
    29581 cgccgtgatc tggcggagcc gtggtttctg gaacatcgaa aagggcgcca gttggcgcaa
    29641 tccggggttt tcgcaatcag acgaagatcc cgttgtggcc gtgagctggc acgacgcagt
    29701 cgaattctgt cggtggctgt cgcgtgagtc aggcgaccga ttccggctgc cgaccgaggc
    29761 cgagtgggaa tacgccgcac gtgctggaac taaggcggaa cagccatccg atctcggcga
    29821 ggcggcgtgg tacaaggtca actcggacgg gacgactcac cctgccgggc ggaaaaagcc
    29881 caacccgtgg ggcttgtacg atatgcttgg caacgcttgg gagtgggtag aggatgtctg
    29941 gtcgaagaac tacgacggag ccccgaacga tgggacaccc agagtcgacg gagggagcgg
    30001 attcgcatct ctcgcgcccg gcgaggtacg ccccctgcgg ggtgggggtt ggtgcctcga
    30061 aagcgctgag gctcgctaca gcgcccgccc agggttcggc ttgcaccagc ggtgcaacaa
    30121 tagtggcttc cgggttgccc gcgagcttcc gccgccacgg agctaattgc cctgcgagtc
    30181 cttttcaagt tgttgcgcct cgtgttcctc cgcgccttgt ggcccgagcc aatcctcggg
    30241 tagtgctccc ggggctcacc cgtccgtcac gccaccggca ggtgctgata cctcctcacc
    30301 gcgcaccagc ggcagatgaa gaccagcagc gacttcgccg ccgctaccgc cgtgtcgatc
    30361 tccaggtact ggtccgcgtt gtgagtgttc ccgcctggcg aa
//
LOCUS       DATIFQ010000075         5156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_598626, whole genome shotgun sequence.
ACCESSION   DATIFQ010000075 DATIFQ010000000
VERSION     DATIFQ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598626"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VLE22_03320"
     CDS             complement(<1..761)
                     /locus_tag="VLE22_03320"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB13461.1"
                     /translation="MFKTLGDWGRAGLVLALLLAVTLPVLGQERFGNLSGTATDPSGA
                     VLPGVTVTATNKATNRVDTAKTRGDGSYMVPDVEPGRYMVRFEKSGFSRYEVPDVIVL
                     VGKTSRVDASMQVGSLEQVVQVTEAAPLIDTSSTMIAHNVTSEEFSRLPKARTFQGIA
                     IFSTSVNTGEIEGGYQVNGASSAENNYYIDGVSTNSVIDGSARQAAAFEYLQEVQVKT
                     AGLEAEYGGALGGVVSAITKSGGNDFHGEIHYYYYG"
     gene            1288..3372
                     /locus_tag="VLE22_03325"
     CDS             1288..3372
                     /locus_tag="VLE22_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB13462.1"
                     /translation="MTKRRLLYGSGVVALAVLVTLIVWQGSFTFGDYGPANPAQTLLY
                     WAVSSLVFILTLTLAFILFRTGVKLYIEHRSGREGSRIRTKLVIGALALSFLPVFFLV
                     LWSVYVLNRNLDKWFSRPAEGIKLNLVEIVESIEREAQARVKAQAELLAVLPEIRAIE
                     GAPAGASGPPPGFFDRFCRDHGMEAAVLEHTDGTRTTLCEGRAEGSDSVIVASVPTAG
                     GRLVVAALKPVDLVRKRDEIQSYLTAYDQLALDRRSARNFYLQLLFLLTLFILFLATW
                     IALFLARLISVPISALLVAAGEVRKGNLGYRLEVRAADELATLVRGFNEMTEALETGR
                     RELESRRRFTEAILESIPTGVISLAPDGRILRVNSALEQIFPEERVARAERLEDLFSR
                     EDTAEIKYMMKRARRTGVASNQMELSTGRGNLHLSVTVSALEAKITSGFVLVLEDTSE
                     LLRAQKAAAWQEVARRIAHEVKNPLTPITLSAQRVSRQLERAQIPAETARIVGECTTT
                     IVQEAESLKRLLDEFSQFARFPAAQPAPSDLNEVVESALNVFAGRLEGIQVHKELAPG
                     LPPVNIDRDQFRRVVTNLVDNSAEAMQESPVKRLFVATHAMAPDAVELVVADTGRGVS
                     TAEKEKLFLPTFSTKGRGTGLGLAIVSRILTDHNAHIRVEDNHPAGARFIIEIPALAA
                     SETETNPAEVRA"
     gene            3369..4730
                     /locus_tag="VLE22_03330"
     CDS             3369..4730
                     /locus_tag="VLE22_03330"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB13463.1"
                     /translation="MNRPRVLVVDDEPAIRQSLCGVLADEGYEAHAVASGEDCLTELG
                     RGFELVLLDIWLPGIDGMETLARIQEIPFGDRPVVVMISGHGTIETAVKATKLGAFDF
                     LEKPLTIEKVSVVAKNALRHRELELENQQFKKDEQCRYRIIGESVPMKALRQQMALMA
                     GTNGRVLIFGESGTGKELVAHALHAMSARAAQPFVEVNCAAIPEELIESELFGHRKGS
                     FTGAHEDKAGKFHKADGGTLFLDEVGDMSLKTQAKVLRALDEQRFEPVGAPESMQVDV
                     RVVAATNKHLEEEIERGNFREDLFYRLNVIPFYVPPLRERAEDVPLLAEYFLREFTTA
                     YGRKAKELTPEAYRVLRDYYWPGNVRELRNLMERIVIMNPQVRIDARHIPLNPARRTT
                     VERSLDGFGSLHEVRAAAEREYILRKLDETNGNVSRAAELLGLERSNLYRKMKTLGIA
                     PKE"
     gene            4768..>5156
                     /locus_tag="VLE22_03335"
     CDS             4768..>5156
                     /locus_tag="VLE22_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13464.1"
                     /translation="MKHTLKLLFLVTLLSAAALAQPVVDAVLHSASYALPGMPNYGIP
                     QGGIFTVFGRNLGPGGLKQVTAFPLLPNWEGITIKVTVDGTTVDAVPISYAAVNAASS
                     TQVSAILPSNTPNGDGTLVLSFNNQPSA"
BASE COUNT         1023 a         1531 c         1623 g          979 t
ORIGIN      
        1 ccgtaatagt aatagtggat ttcgccgtgg aaatcattgc cgccggactt ggtgatcgcg
       61 ctgactacgc cacccaacgc tccaccgtat tcggcctcca ggccggcggt tttcacctgg
      121 acttcctgaa ggtactcgaa cgcggcggcc tggcgagcgc tgccgtcgat gacactgttc
      181 gttgaaacgc cgtcgatgta gtagttgttt tcagcggagc tggcaccgtt gacttgatag
      241 ccaccctcga tttcaccggt gttcaccgaa gtcgagaaaa tggcgatgcc ctggaaggtg
      301 cgtgccttcg ggaggcggct aaactcttcc gaggtcacgt tgtgagcgat catggtgctc
      361 gacgtgtcga tcagcggagc ggcctcggtc acctgtacca cctgctccag gctgccgacc
      421 tgcatggagg catcgactct cgaggttttg cctaccagaa cgataacgtc cggaacttcg
      481 tatctcgaga atcctgattt ctcgaaacgg accatatatc ggcccggctc cacatccgga
      541 accatgtagc ttccatcgcc gcgtgtcttg gcggtgtcga cacggttggt agctttattg
      601 gtcgccgtga cggtgacgcc cggaagaacc gcaccgctcg gatcagtggc cgtgccgctg
      661 aggttgccga agcgctcctg cccgagcacg ggcagagtca ccgcaaggag aagtgcgagc
      721 acgagacccg cacgtcccca atcgcctaag gttttgaaca taaactacct cctcaatgaa
      781 attcctttgc ggactaaggt atccatgtac ctttgatgac tgaagtcttc ccctgcctgc
      841 aatcgtcggg ccgaaatgtc aacattcagc gctccaatag cttgcaagcc gcgccaaatg
      901 cctccggtta tgagaatgcg gagtttgtaa cccaaagatc ttatatgcta accgaagata
      961 ggcgctactc aaagctccaa gccaaaagcc gccaacgccc tatacggcac ccacccctgg
     1021 gtgctgtata ggagttgagg caaatcatca ggattcctgt agcctaatcc ctacccgata
     1081 gcgaatagga ttgtagcaca tggatcgcca agattcgtcc tcacggattc aaaaggttac
     1141 acgttaaatt ctattaagct tacaagatcc catatccctg cgtcttggat tcggtaggat
     1201 gtcgcgccta atacctgatt tttgggtgct ggtaccccaa ccgggcgtgc tcgcgcaccc
     1261 taccgtataa tcgggaggag gttgatcttg accaagaggc gtctgctcta cggctccggc
     1321 gtcgtcgcgc tcgcggtgct ggtgacgctg atcgtgtggc aggggtcttt cacgttcggc
     1381 gattacggcc ccgcgaaccc ggcccagacg ctgctttatt gggcggtctc gagcctcgtt
     1441 ttcattctca cgctcacgct cgccttcatt ctgttccgga ctggcgtgaa gctctacatc
     1501 gaacaccgga gcgggcggga aggatcgagg atccgcacca agctcgtgat cggggccctc
     1561 gcgctcagct ttctgccggt attctttctc gtcctctgga gcgtctacgt cctgaaccgg
     1621 aacctcgaca agtggttcag caggccggcg gaaggcatca agctgaacct agtcgagatc
     1681 gtcgaatcga tcgaacgcga ggcccaggca cgggtcaagg cacaggcgga actgctcgcc
     1741 gttctgccgg agattcgagc gattgaaggg gctccggccg gcgcgtccgg tccgccacca
     1801 ggcttcttcg accgattctg ccgcgaccac ggaatggagg cggccgtgct ggagcatacc
     1861 gacggcacca ggaccacgct gtgcgaggga cgggcggaag gctcagattc ggttatcgtg
     1921 gcgagcgttc caaccgccgg tggccgattg gtggtcgcgg ctctgaagcc cgtggatctt
     1981 gtcaggaagc gggacgagat tcagagttac ctgaccgcct atgaccagct cgccctcgac
     2041 cgcaggagcg cccgaaactt ctatctccaa ctgctgtttc tgctgacgct gttcatcctg
     2101 ttcctggcga cctggatcgc gctcttcctg gcccggctga tcagcgtgcc gatctccgct
     2161 ctgttggttg ccgccggcga ggtgcggaaa gggaacctcg gctaccggct cgaggttcgc
     2221 gcggccgacg aactggccac gctcgtccga ggcttcaacg agatgaccga agcgctcgag
     2281 acgggccggc gcgaactgga gagccggcgg cgctttaccg aagcgatcct cgagagcatc
     2341 ccgaccggcg tgatttcgct tgctccagac ggcaggatcc tgcgcgtcaa cagcgcgctc
     2401 gagcagatct ttcccgagga gagagtagcc cgggcggaac ggctcgaaga ccttttctca
     2461 cgggaggaca ccgccgagat caaatacatg atgaagcgcg cacgccgcac cggcgtcgca
     2521 tcgaatcaga tggagctgtc caccgggcgg ggcaatcttc atttgagcgt cacggtgtcg
     2581 gcgctggagg cgaaaatcac ctcgggattt gtcctggtgc tggaggacac gagcgagctg
     2641 cttcgggcgc agaaggccgc ggcctggcag gaggtggcgc ggcgcattgc gcacgaggtg
     2701 aagaacccgc tgacgccgat cactctttcg gctcagcgtg tgagccggca gctcgagcgg
     2761 gcccagatcc cggcggaaac cgctcgaatt gtcggcgagt gcactaccac gatcgtacag
     2821 gaggcggaat cgctcaagcg gctgctcgac gaattctcgc agttcgcccg cttcccggcg
     2881 gcgcagcccg cgccgtccga tctgaacgaa gtggtcgaaa gcgcgttgaa tgtgtttgcc
     2941 gggcggctcg agggaatcca ggtgcacaag gaactggcgc cagggctccc gccggtcaac
     3001 attgatcgcg accagttccg gcgcgtggtg accaaccttg tcgacaactc cgccgaagcg
     3061 atgcaggaat cgcctgtaaa gcggctcttc gttgccacgc acgcgatggc gccggacgcg
     3121 gttgaactgg tcgtcgccga caccgggcgc ggggtctcga cggcggaaaa agaaaagctg
     3181 tttctgccca cattttccac caaggggcgc ggcaccggac tcggcctcgc catcgtcagc
     3241 cggatcctga ccgaccataa cgcgcacatc cgggtggagg ataaccatcc cgcgggtgct
     3301 cggttcatta tcgaaatccc ggcgctggcg gcgagtgaga cagagacaaa cccggcggag
     3361 gtgcgtgcgt gaatcgcccg cgcgtccttg tggtggacga cgagccggcc atccggcaat
     3421 cactctgcgg cgttctcgcc gatgaaggct atgaggccca cgccgtcgcg agcggcgagg
     3481 attgcctgac ggagctggga cggggtttcg agttggtgct cctggatatc tggctgccgg
     3541 gaatcgacgg catggagacg ctcgctcgca tccaggagat ccccttcgga gaccggccgg
     3601 tggtcgtgat gatttccggg cacggcacca tcgagaccgc ggtaaaggcg acgaaactcg
     3661 gcgcgttcga tttcctcgaa aagccgctga cgatcgagaa ggtatcggtg gtggcgaaga
     3721 atgcgctgcg tcaccgggaa ctggaactgg aaaaccagca gttcaagaag gacgagcagt
     3781 gccggtaccg gatcatcggc gaaagcgttc cgatgaaggc cctgaggcag caaatggcgc
     3841 tgatggccgg caccaacggg cgggtgctga tcttcggaga gagcggaacc ggcaaggaac
     3901 ttgtggcgca tgcgctccat gcgatgagtg cgcgggcggc gcagccgttc gtcgaagtga
     3961 attgcgccgc gattcccgag gagctgatcg agagcgaact tttcggacac cgcaagggca
     4021 gcttcacggg cgctcacgag gacaaagccg gcaagttcca caaggccgac ggcggtacgc
     4081 tgttcctcga tgaggtcggc gacatgagcc taaagacaca ggccaaggtg ttgcgggcgc
     4141 tcgacgagca gcggttcgag ccggtcggcg cgccggagtc gatgcaggtg gacgtccggg
     4201 ttgtcgcagc caccaacaaa caccttgaag aagagattga acgcggtaat ttccgcgagg
     4261 atcttttcta ccggctcaac gtgattcctt tttatgtgcc cccgttgcgc gaacgggccg
     4321 aggatgtgcc gctgctggcc gagtattttc tgcgggagtt caccacggcg tacggccgca
     4381 aggcgaagga actcacgccg gaggcgtacc gcgtactccg ggactactac tggccgggca
     4441 atgtgcggga actgcgcaac ctgatggaac ggatcgtgat catgaatccg caggtgcgga
     4501 tcgacgcccg gcacatcccg ctgaatccgg cgcggcggac gacggtggag cgttcgctcg
     4561 acggtttcgg gagcctgcat gaggtgcgag cggcggccga gcgggagtat atactaagga
     4621 agctggatga aaccaacggg aacgtttctc gggcggctga gctactgggg ctggagcgaa
     4681 gcaacctcta ccgaaagatg aagacgctcg ggatcgcacc caaagagtag tctgggttct
     4741 ccacaactcg aatcaatcgg aggcaacatg aagcacacgc tcaagctgtt gttcctggtc
     4801 acgttgctat cggcggccgc actggctcag ccggtggtgg atgcggttct gcactccgcc
     4861 agctacgccc tgcccggcat gcccaactac ggaatcccac agggtgggat tttcactgtc
     4921 ttcggcagga atctcggacc gggtggactg aagcaggtca cggccttccc gctgctcccg
     4981 aattgggagg ggattaccat caaggtgacg gtcgacggca caacggtgga tgccgtaccg
     5041 atctcctacg cggccgtcaa tgcggcgtct tcgacgcagg tatcggcgat cctgccttcg
     5101 aacacgccta atggcgatgg cacgctcgtc ctctccttca acaaccagcc gagcgc
//
LOCUS       DATIFQ010000076         4406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2706266, whole genome shotgun sequence.
ACCESSION   DATIFQ010000076 DATIFQ010000000
VERSION     DATIFQ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2706266"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VLE22_03340"
     CDS             complement(<1..493)
                     /locus_tag="VLE22_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSB13465.1"
                     /translation="MHTNRLAQEKSPYLLQHAHNPVDWHAWGEEAFDVARREDKPIFL
                     SVGYSTCHWCHVMERESFEDDRIAEILNRDFVPVKVDREERPDVDRIYMLFVQATTGS
                     GGWPMSVWLTPDLKPFFGGTYFPPDNRYGRPGFALILERIAEAWRTDRARLVESGAAI
                     VEEL"
     gene            complement(504..1427)
                     /gene="dapA"
                     /locus_tag="VLE22_03345"
     CDS             complement(504..1427)
                     /gene="dapA"
                     /locus_tag="VLE22_03345"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HSB13466.1"
                     /translation="MPASGMRGIIPAVVTPFHKDESIDYTAWQHLIEYLIASGVHGLF
                     VIGGQGEFFALNEEERTVATRFCVQTVNRRVPLYANAGAVTTRETIRLAEKAEGEGVD
                     YIVVITPYYLKPSADELVDHYVEVCRSVRLPVLAYNIPERTGVELTPLILQRVAASAE
                     NFVGLKDSSGRLDLMPDWVSAKLAVFIGRDHMILEALKLGAVGAVTACANVVPRAFVD
                     LYNAFAAGNLEEAARLQALVEPLRKAFALATFPSVVKEAMSIAGMPVGCCRRPAGPMP
                     DSARDKLAAVLDKLREERYLPDRAAWTAGVR"
     gene            1485..2840
                     /locus_tag="VLE22_03350"
     CDS             1485..2840
                     /locus_tag="VLE22_03350"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HSB13467.1"
                     /translation="MNYYERNRDAFLKGLMEFLRIPSISTLPENKPDIQRAAEFVAAE
                     LRGAGMQQVEIIEGQGHPLVYGEWTGAPGKPTLLMYGHYDVQPPDPLEEWKSPPFEPT
                     LRGDDLFARGSSDDKGQTYILIKAVEGLLKTEGRLPVNVKFLIEGEEEVGGEHIEAYV
                     SSKPPRLKADAAVICDTEMFAPGLPTLCVGLRGIVYLELDVRGADHDLHSGSYGGAAP
                     NPFQAVAEILCALKDREGHILIPGFYDRVRPPSAKEREAWARLPFDEEEYKRKEVGVT
                     ELVGEPEIPLFERLWARPTLEVHGIRGGFTGEGAKTVIPARAVAKISMRLVPDQRPEE
                     ALEQFTRAVAAACPSGVTAEVRNIHGAAASLVDTESPFIKAAAEALSESFGKETVYIR
                     SGGSIPIVGLFDRFLGIPSVLMGFGLPDDNLHAPNEKFHLPNFYRGIEAVAKYLEVLG
                     R"
     gene            2844..4370
                     /locus_tag="VLE22_03355"
     CDS             2844..4370
                     /locus_tag="VLE22_03355"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HSB13468.1"
                     /translation="MRFQLWPVAFLLCAPAFSQQRLEVIVKDAETNRMMPARVYLTDS
                     KGELHVPPGAITYKKYKEEHFITDGRFRIDLPSGEYTLTVERGLEYVPRTVRLLNTTS
                     PQHFDLALKRWIPMNKLGWYSGDLHNHRKIDEMPQLLLAEDLNLAPTLTDWIWEDRPV
                     SRPPDTTEAIRRVDATHVFSVLDKEVERLQKGPGAIDLVGLKSAIPFEGYWLYPPNDH
                     YCKLAREQGAYIDAEKILWRDSPAIAALCPMDFGGIVHNHFNRHGVEVETDKWGFGPK
                     FRPEFGTPAGMPLWAMEVYYKFLNCGFRIAASAGSASAVKAAPLGYNRVYVKLDGPFS
                     YEAWFRALKQGRSFATNGPVLLLTVNDRIPGDTIEAPSGSVRLNIKANAETIGQLDRL
                     EIVFKGRVVKTVSGGADSDELSAEVELPVNETGWVAARAFERPGETIRFAQTSPVYVK
                     VGDDRGIVRDDAKFFIDWIDIQMEHYRREPGFRSPEHREAMLDYFNRAREVYKKLAAQ
                     "
BASE COUNT          864 a         1413 c         1346 g          783 t
ORIGIN      
        1 ggagttcctc gacaatcgcc gcgccggact cgaccagcct ggctcgatcc gtccgccaag
       61 cttccgcaat gcgctccaga ataagcgcga agcccgggcg cccgtagcgg ttgtcgggag
      121 gaaagtacgt gccaccaaag aacggcttca ggtccggcgt gagccagacg gacatgggcc
      181 agccgccgct tcccgtcgtc gcctgaacga agagcatgta gatgcggtcg acgtccggcc
      241 gttcctcgcg gtccacttta accgggacaa aatcccggtt caggatctct gcgattcgat
      301 cgtcctcgaa cgactcccgt tccatgacgt ggcaccagtg acatgtggag tagccgactg
      361 acaggaagat gggcttatcc tcccggcgcg ccacgtcgaa cgcctcttcg ccccaggcgt
      421 gccagtccac cggattgtgg gcgtgttgaa gcagataggg actcttctcc tgtgcgagtc
      481 gatttgtgtg cattcccccg aagctacctg acaccggccg tccaggcggc ccgatccggc
      541 aagtagcgct cctctctgag tttgtcgagc acggcggcga gcttgtcgcg agcggagtcg
      601 ggcatcggcc ccgctggccg ccggcaacat cccacgggca tcccagcgat gctcatcgcc
      661 tctttcacca ccgaaggaaa cgtcgccagc gcgaacgcct tccgaagggg ttccaccagc
      721 gcctgcaagc gcgccgcttc ttccaggttt cctgccgcga acgcattgta caggtctacg
      781 aacgcccgcg gcacaacgtt cgcgcaggcc gtgacggcgc cgacggctcc cagtttcagc
      841 gcctccagga tcatgtggtc tcgcccgatg aacaccgcca gcttggccga aacccaatcc
      901 ggcatgaggt ccagcctgcc actcgaatct ttcagcccga cgaaattctc ggcggaggcg
      961 gctacccgct gcaagatcag cggcgtgagc tcgacgccgg tgcgctccgg gatgttgtac
     1021 gcgagcaccg gcaggcggac gctgcggcag acctcgacgt agtggtccac aagttcgtcg
     1081 gcggacggct tgaggtagta cggcgtgatc accacgatgt agtcgacgcc ctcgccctca
     1141 gccttctccg cgagacggat ggtctctcgt gtggtgacgg cgccggcgtt ggcgtaaaga
     1201 ggaacccgac ggttcaccgt ctgcacgcaa aagcgagtcg ccaccgtgcg ttcctcttcg
     1261 ttgagagcga agaactcgcc ctggccacca atcacgaaga gcccgtgcac gccgctcgcg
     1321 atcaggtatt cgatgaggtg ctgccacgct gtatagtcga tggactcgtc cttatggaac
     1381 ggcgtcacta ctgccggaat gatcccccgc atcccgctgg caggcatgtc tttattctag
     1441 cccacctcac ctcacggcct acccgttgtt atcattcctc tggtatgaac tactacgagc
     1501 ggaatcgcga tgcttttctg aaggggctca tggagttcct tcggatcccc agcatcagta
     1561 ccctccccga gaacaaaccg gacatccagc gcgcggccga gtttgtagcc gcggagctcc
     1621 gcggggccgg catgcagcag gtcgagatca ttgagggcca ggggcacccg ctggtgtacg
     1681 gcgagtggac cggcgccccg ggtaagccga ccctcctgat gtacggccac tatgacgtcc
     1741 agccacccga tccgctcgaa gagtggaagt cgccgccgtt cgagccgacc ctccgcggcg
     1801 acgatctgtt cgcgcgaggc tcgagcgacg acaagggaca gacgtacatc ctcatcaaag
     1861 ccgtggaggg gctcctcaag acggaaggac ggctgccggt caatgtgaaa ttcctgatcg
     1921 aaggcgaaga ggaggtaggc ggcgagcaca ttgaggctta tgtgtcgagc aaaccgcctc
     1981 gcctcaaagc cgacgccgcc gtcatctgcg acaccgagat gttcgctccg ggcttgccaa
     2041 ccctttgtgt cggcctgcgg ggcatcgtct atctggaact cgacgtccgt ggcgcggacc
     2101 acgacctgca ctccggatcg tacggcggcg cggcgccgaa tcccttccaa gcggtggctg
     2161 agattctctg cgcgctgaag gaccgcgagg ggcatatcct gatcccggga ttctacgacc
     2221 gtgtccggcc accgagtgcg aaggaaaggg aagcctgggc gcggctcccg ttcgacgagg
     2281 aggagtacaa gcgcaaggaa gtcggtgtca ccgagcttgt gggtgagccg gagatcccgc
     2341 tattcgagcg gctctgggcg cgaccaacgc ttgaagtcca cggcattcgc ggcggcttca
     2401 cgggagaggg cgccaagaca gtcattcccg cgcgcgcagt cgccaagatc agcatgcgcc
     2461 tcgtgccgga tcagcggccc gaagaagcgc tcgaacagtt cactcgcgcc gtcgcggcag
     2521 cctgcccaag cggcgtgacc gcagaggtgc gaaacatcca cggcgcggcc gcgtcgctgg
     2581 tcgacacgga gagccccttc atcaaggcgg cggctgaggc actgtcggag agcttcggca
     2641 aggagaccgt gtacatccgc tcgggcggct cgatcccgat cgtcgggctg ttcgaccggt
     2701 tcctcggcat cccaagcgtg ctgatgggct tcggcctgcc ggacgacaac ttgcacgcac
     2761 cgaacgagaa gttccacctg ccaaacttct atcgaggtat cgaagcggtg gcaaagtacc
     2821 tcgaggtcct ggggaggtaa ccagtgagat tccagctatg gcctgttgct tttctgctat
     2881 gtgccccggc gttctctcaa caaaggctcg aagttattgt caaagacgcc gaaaccaacc
     2941 gcatgatgcc ggcccgggtc tacttgacag actccaaggg agagcttcac gttcccccgg
     3001 gcgccatcac atacaagaag tacaaggaag agcatttcat tactgacgga cgcttccgga
     3061 tagacctacc ttccggagag tacacactta ccgtcgagcg gggtcttgaa tacgtcccaa
     3121 gaacggttcg gctgctcaac acgacgtcac ctcagcattt cgatctggcc ctcaagcgtt
     3181 ggatcccgat gaacaagctc ggctggtatt ccggcgacct ccacaaccac cgcaagatcg
     3241 acgaaatgcc gcaacttctc ctcgccgagg acctgaacct cgcaccgacg ctcaccgact
     3301 ggatctggga ggacaggccg gtctcgcgcc cgcccgacac gacagaggca attcgccgcg
     3361 tggacgccac gcacgtcttc agcgtgctgg acaaggaggt cgagcggctc cagaaaggcc
     3421 ctggcgcgat cgacctcgtc gggctgaagt cggcaattcc gttcgagggc tactggctct
     3481 atcctcccaa cgaccattac tgcaagcttg cccgcgagca aggcgcctac atcgacgccg
     3541 agaagatcct gtggcgcgac tcgccggcca tcgccgcgct ctgcccgatg gacttcggcg
     3601 gcatcgtcca caatcacttc aacaggcatg gcgtcgaggt ggaaacggac aagtggggat
     3661 tcggaccaaa gttccgcccg gaatttggca cgccggcggg gatgccgctc tgggccatgg
     3721 aggtctacta caagtttctg aattgcggct ttcgcatcgc ggcgtccgcg ggcagcgctt
     3781 ccgcagtcaa ggccgcgccg ctcggataca accgcgtcta cgtgaagctc gatgggccgt
     3841 tctcgtacga ggcctggttc cgagcgctga agcagggccg cagcttcgca accaacggac
     3901 ctgttctcct tctcaccgtg aatgatagga ttcccggcga cacaattgag gcgccctccg
     3961 gctctgtgcg gctgaacatc aaggccaacg ccgagacgat cggtcaactc gaccgcctcg
     4021 aaatcgtttt caagggccgt gtcgtaaaga ccgtctccgg tggtgcggac tcggacgagc
     4081 tgtcggcaga agtggagctg ccggtgaacg agaccggttg ggtggcggca cgtgcatttg
     4141 agcgcccggg agagacgatc cggtttgcgc agacaagccc ggtgtatgtc aaggtcggag
     4201 acgaccgggg catcgtacgt gacgatgcga agtttttcat cgactggatc gacatccaga
     4261 tggagcacta ccggcgggaa ccaggatttc ggtcgcccga acaccgcgag gccatgctcg
     4321 actatttcaa ccgcgcccgg gaggtgtaca agaagctggc cgcgcaatag cggtccccac
     4381 cgtccgaaaa aagtgctatc atccct
//
LOCUS       DATIFQ010000077         4433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1365190, whole genome shotgun sequence.
ACCESSION   DATIFQ010000077 DATIFQ010000000
VERSION     DATIFQ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365190"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VLE22_03360"
     CDS             complement(<1..433)
                     /locus_tag="VLE22_03360"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HSB13469.1"
                     /translation="MAQEKDVASGILEAAARHDRLVIAGALGFVSLLAWAWLLTGGGM
                     DMPSSMDMNMVMPKDWTPLYALVVLVMWWVMMIAMMLPSAAPMILLFSLVNRRSRVPG
                     APYVPTAVFATGYLVAWGAFSLAATLVQWCLEQAGWLTMGMT"
     gene            complement(459..1100)
                     /locus_tag="VLE22_03365"
     CDS             complement(459..1100)
                     /locus_tag="VLE22_03365"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HSB13470.1"
                     /translation="MLMATIDWRIRGPEIATCNCNWGCPCQFNSRPTHGHCRAGVAMQ
                     IDEGHFDGTSLDGLRWAAIFAWPKAIHEGNGEALLILDERADETQREALLSILSGQTS
                     VPGATIFNVFAATLSTLHDPVTAPIEFELDRDNWTGHFSVPGYLDAHAAPIINPVSGE
                     SHRARVMLPDGFEYHEAEFVSSRTRGEGPIPLDWTDGHGHICMLHMTPNGPVD"
     gene            complement(1582..1848)
                     /locus_tag="VLE22_03370"
     CDS             complement(1582..1848)
                     /locus_tag="VLE22_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13471.1"
                     /translation="MMIKDLEVRKDLTSDELSAVRGGDNFSYQGGQFAPAVGLSGPSF
                     FSSQTVTNAAVNSPVAVQNDNDLNLSLENKTANVLQSLGTLVVQ"
     gene            complement(2430..2699)
                     /locus_tag="VLE22_03375"
     CDS             complement(2430..2699)
                     /locus_tag="VLE22_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13472.1"
                     /translation="MKTVKIDDLETRKDLDAATMKTISGGNLIGQGGQFTPVTAIGGG
                     IFSPQVVTNVPVNVPIAIQLDLDTNLGIDTKVANVIASAASGITQ"
     gene            3163..3471
                     /locus_tag="VLE22_03380"
     CDS             3163..3471
                     /locus_tag="VLE22_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13473.1"
                     /translation="MSTLVINDLALSKDLDTAAVMKICGGFSLGWTVARQEPPPSSRA
                     LVPSSIYNITNNYIDYDYHVINQNPTIFNVYNGAGNSGTIVNSFSTLSVNAASPALMA
                     "
     gene            complement(3789..4427)
                     /locus_tag="VLE22_03385"
     CDS             complement(3789..4427)
                     /locus_tag="VLE22_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSB13474.1"
                     /translation="MVHEVQPLSLFLNGITALFSLFGFARSGLVAWRPAILLAIVTTV
                     FAPFGAWLAQIVDARWLWAFYFAAVAFLAWRMFQPEKSGATTTAEPNMKLALLLAAPI
                     AIAAGLLGVGPGFLLLPSLILLGFEPKHAAAINSLAVTPPSFSALIPHLATAKVDLAL
                     GAVLVVVGALCSFLGARITSLYVPGKRLKQAFGVLIVVMTGYKLFQILGGSG"
BASE COUNT          867 a         1342 c         1428 g          796 t
ORIGIN      
        1 tggtcatgcc catggtcagc cagccagcct gctccaggca ccactgcacc agggtcgccg
       61 caaggctgaa cgccccccag gcgactaggt agcccgtggc gaacaccgcg gtcggcacgt
      121 agggcgcacc cggcacgcgg ctacggcggt tgaccaggga gaacaacagg atcatgggag
      181 cggcacttgg cagcatcatg gcgatcatca tgacccacca catcacaaga accacgaggg
      241 cataaagcgg tgtccagtcc ttgggcatta ccatgttcat gtccatggac gacggcatgt
      301 ccatgccacc accggtcagc agccaggccc aggcaagcag tgagacgaaa cccagcgcgc
      361 cggcgatcac cagtcggtcg tgtcgcgcgg ccgcttccag tatgccgctg gccacgtcct
      421 tttcttgcgc catgatcctg gcgaccgtgc cgtgaggttc agtccaccgg cccgttgggt
      481 gtcatgtgga gcatgcagat atgaccgtga ccgtcagtcc agtccagcgg gatcgggccc
      541 tcgccccggg tgcggctgct gacgaactct gcttcgtggt actcaaagcc atccggtaac
      601 atcactctgg cgcgatgaga ttctccggag accgggttga tgatgggtgc ggcatgggca
      661 tccagatagc cgggtacgga gaaatgccca gtccagttgt cgcgatccag ctcgaactcg
      721 ataggggcag tcaccggatc gtgcagcgtg ctgagtgtcg cagcgaagac attgaatatg
      781 gttgcacccg ggacggaggt ttggccggag aggatgctca gcagagcctc gcgctgcgtt
      841 tcatcggccc gctcatcgag gatcagcagg gcttctccgt tgccctcgtg gatggccttc
      901 ggccaggcga atatggccgc ccaacgcagg ccgtcgagtg aagtgccgtc gaagtgaccc
      961 tcatcgatct gcatggccac accggccctg caatggccgt gggtgggtct cgagttgaat
     1021 tggcagggac aaccccaatt gcaattgcag gtggcgattt cgggtccacg gatacgccag
     1081 tcaatagtag ccatgagcat tcctccgctt catatagtct taagttttat gtatagcaga
     1141 ggcttgacca ttttgccttc cggctgacag gagaggtgtc ccgtgccagg cgtggaaacc
     1201 gtgggtgtat cggcgcgagg tacgggtaaa aaaacccgcc ggtcagtgat caggcgggtg
     1261 cggggaggtg agcccggggg ggctctgtca gggacgggca gagggagtac tgcgtgggtg
     1321 tggttatgcc gtgcgcgaga caatagcgga ttcaggcgag tgggaaggcc agcgccaatc
     1381 tctactccct tatcgagacg tgcaaggtca cgctctggaa ccctatgcct atctgcgcca
     1441 gcgtcaggcc gtttccggca gccggccgcc atcgatcctt gcaacctcgc cggttgtgat
     1501 tcgatccgaa ccggcggtaa aagcacaacg ggggtccgaa gacccccgtt gtcatgggct
     1561 gcgccgcagg cggcggataa cttattgcac gaccagcgtg cccagggact gcaggacgtt
     1621 ggcggtcttg ttttccaggc tgaggttcag gtcgttgtcg ttctgcaccg caaccgggct
     1681 gttcaccgcg gcattggtca ccgtctgcga gctgaagaag ctcgggccgg agaggccgac
     1741 ggcgggggcg aactggccgc cctgatagct gaagttgtca ccaccgcgca ccgcggacag
     1801 ctcgtcgctg gtcaggtcct tacgcacttc caggtcttta atcatcatgg tcaggttctc
     1861 ctaaagtcag ttgcaaaaat gggttgggtc agggttgcct ctcggcgttg tgtgaacgtt
     1921 gcggttcacg tcccctctat tgcagcggcc gtgccagctt tcggcggcgg cgcgaaaata
     1981 aatcacaagc gattgagaac tatgtgttta ttatttttat gcggcggcga ggtcacggtg
     2041 cagggcggcg ctaaccggcc gcgtcgttcg ctcgccacca acaccagcgg gggcgaagtt
     2101 tgcagactgg caacacctcg gcgggcgaca ccccccgagg cggcgagaca ctgccatccg
     2161 gcggcccggc agcggtcacg ctgtgggtga tggcgcaggt gataagtggc gcggcgggaa
     2221 gcagcgcggc aaccgctgcc ggagatccgg cgatagccgg ccgcggcgcg ggaagccccg
     2281 cctgccaggg tgtgcggatc ggcggggtcg tttccggcgc cggctcaagc cggtgtctat
     2341 caggggaggg aagtgcggga gtagcaagct acacggaggt ccggcgggac ctccgcgtag
     2401 ctggcggcac ggcgtgccgc cctggagtgt tactgggtga tacccgaggc ggctgaggca
     2461 atcacattgg caaccttggt atcaataccg aggttggtat ccaggtccag ctggatcgcg
     2521 atcgggacat taaccggcac gttggtgact acctgcgggc tgaagatgcc cccgccgatg
     2581 gcggtcaccg gggtaaactg gccgccctgg ccgatcaggt tgccgccgct gatggtcttc
     2641 atggtggcgg cgtccaggtc cttgcgggtt tccaggtcgt caatcttcac agtcttcata
     2701 agcaaatctc ctaaacttgt cacgggttgg ggtgggtcgg ggtgttaacc gtgtgggcac
     2761 cgcggtgcgc acgcccacta ccaaggcagg atccgtacca gttctgtcgc cgccgccgcc
     2821 gtggcggtgt agctccctgt tttttcttgt gaataacatc ggcccgacgg cgcggcgcgg
     2881 tccccggagg cgctcgaggc gccgcggaag tgggtcccgg acaaacactc gtccccggac
     2941 tgttgggcgc cgccggcgtt tgcgcggcac ccgggcgcgg cttgggcgcg gggaaagtgg
     3001 gccaggtacg ttatttgttc ttgctaacca ggggcttatc ctgaccgtcc gccaggcgcg
     3061 gcggtattgg cacggctcct gctcagtatc tggtcagccc gcggcggcaa cccgccacgc
     3121 cgcaaaccgc gaatctgacc aacagaacca ggagacccga ccatgagcac actcgtcatc
     3181 aacgatctgg cgctaagcaa agacctcgac acggccgccg tgatgaagat ctgcggcggc
     3241 ttcagcctcg gctggaccgt cgcccgccag gaaccgcccc cctcctcccg ggcgctggta
     3301 ccaagcagca tctacaacat caccaacaac tacatcgatt acgactacca cgtgatcaac
     3361 cagaatccga ccatcttcaa cgtctacaac ggcgcgggca actccggcac catcgtcaac
     3421 agcttcagta ccctgtccgt gaacgccgcc agcccggcac tgatggcctg accccgacgc
     3481 gagtgaaacc cgatgcctcc cccttgcgga gttcctggtg aactccgctt ttttttgggg
     3541 gcacaacaga tcagaccccg accttcctgg attctcgctt ccgcgggaat gccagaaatt
     3601 ccggatctct tgccttcacg ctgagtaagt gccatgcggt gctgacccgt gtggtacttg
     3661 cttactcata cccgccagcg tcagttgaat gaccagaccg tgtgaaggta gtgttgcgcc
     3721 tgcggacatg gatcacggac attcgctgac aagaccaagc gaaccagaga gcgacacact
     3781 atagcttctc atcccgagcc accaagaatc tggaacaact tgtagccagt catcaccacg
     3841 atcaacacgc caaaggcctg tttcagacgc ttgcccggca cgtagaggct cgtgatgcgc
     3901 gcgccaagga aggaacacag cgcccccacg acaaccaaca cggcgccaag agcgagatcc
     3961 actttggcgg tggcgaggtg cgggatgagc gcggagaacg agggcggcgt caccgcgagc
     4021 gaattgatcg ccgccgcgtg cttgggttcg aagccgagca ggatgagtga gggcaatagc
     4081 aggaatcctg gccccacgcc gagcagaccg gcggcgatcg cgatcggggc ggcaagcagc
     4141 agggcgagtt tcatattggg ttcggcagtg gtcgtcgcgc cggatttctc tggctgaaac
     4201 atgcgccagg cgagaaaggc gacggcggcg aagtaaaacg cccacagcca gcgcgcatcc
     4261 acgatttggg cgagccacgc gccgaaggga gcgaagaccg tggtgacgat ggcgagcagg
     4321 atcgccggcc gccaggcgac cagaccgctg cgcgcgaagc cgaacaggga aaacagcgcg
     4381 gtaatgccgt tcagaaacag cgacaacggc tgtacctcat gtaccagatc gtg
//
LOCUS       DATIFQ010000078        11344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_25026, whole genome shotgun sequence.
ACCESSION   DATIFQ010000078 DATIFQ010000000
VERSION     DATIFQ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11344
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25026"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1518)
                     /locus_tag="VLE22_03390"
     CDS             complement(145..1518)
                     /locus_tag="VLE22_03390"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB13475.1"
                     /translation="MPRNRILIVDDEENIGRSLRLILEREGYAVTVCRSISDCRAHPD
                     TGRADVFLLDVKLPDGNGIDLLRDLRGTDHCAPAVMISGHATIADAVEATRAGAFDFL
                     EKPLGRDRVLLAVKNALEQANLRQENARLRELVGDGPRMIGGSPAWQRAVEQATMAAR
                     SDARVLLIGESGTGKELLAEQIHRLSPFAPGPFVKVNCAAIPTELLESELFGHEKGAF
                     TGAASARRGKFELADSGTLFLDEVGDLHPASQAKLLRVLQEGEFHRVGGEQTIRVTVR
                     VISATNRDLAALVAKQQFREDLYYRLSVVPVRVPALRERPQDVRPLAEYFLGEFCARN
                     NFRAKAFVDEVFPVFERYGWPGNVRELRNTVERMAILSAGEQLGEDSIPAEVRAARRE
                     GTPSSLQQARETAEREHLVKALSEAEWNVSAAARALGMERTNLHKRIRALGLSRERDP
                     RAANGRE"
     gene            complement(1490..2899)
                     /locus_tag="VLE22_03395"
     CDS             complement(1490..2899)
                     /locus_tag="VLE22_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB13476.1"
                     /translation="MPRSLRGRLILLFVVATIPPLGATLWFTSSLLEWSLTYASTREL
                     DEISRALQRLGREYYRQAQDSLRRDAAQGAIEPQRYLPGAEASWPAEVRAFQESGETE
                     RFVLSGTNGDRLNFMTRRGGEVRVWSRPLGSVGMESLTRQYRQARALVDAGNQRNLRR
                     GFTLTLLLLALSVWLVGFALLAYLASRVSRPIRELSAALSELASGNLAVRVSVDREDE
                     IGEAMHAFNRTAEELEQNRARLVYLAQLASWQALARKMAHELKNSLTPIRLTMEEVLA
                     RAGESDPAFIQQAAQIVGEEVETLERRVRAFSEFAAEPPVRPGVLDVNAVVEERVAFL
                     RTGRPEVRYTLRLDPRLPAAHTDEDLVKGVLTNLLENAAEAAGAGGEVLGRTSLLNGK
                     VAVEVHDSGPGLSEDVRRSLFEPAISFKKGGMGLGLSIARKSALLAGGDILLVKGELG
                     GAAFRVVLPRYAAEQNPDR"
     gene            complement(2919..3485)
                     /locus_tag="VLE22_03400"
     CDS             complement(2919..3485)
                     /locus_tag="VLE22_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13477.1"
                     /translation="MKLATAGHLALLAAAQALYGQGLEVRLGTGTLRVAAPNFHFVEG
                     RPLEQLRNGNSVIFAVQLTLLGDRKTTVIDRTGGRFAMSFDLWEEKFAVTRLGGTRKA
                     TSHLAAGAAEAWCLDNVSVSRARLSPSSPFWVRLEVRAEEPNGDTPPEENAGINLARM
                     VEFFSRSPHPGESRWLAEGGPFRLAELK"
     gene            complement(3639..4883)
                     /locus_tag="VLE22_03405"
     CDS             complement(3639..4883)
                     /locus_tag="VLE22_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HSB13478.1"
                     /translation="MKYGWLIALVMASGLGASDAGSQVNVNSRYRVERVDISDNGINR
                     ISRTLREDLDRLVGEKLNPQKLDRLAQRIRRELNARLVQQKLERGTQPEQVIVLFEVT
                     GRRPRQNFDASMTRGVYHAKQGWSGAIGTTTTVGTNRFSLGIVSDGDELLERYAGVRA
                     GYENTKVGTERVRLGFRFASYHQKWNADTQAALAARSDVPGVYRTRQDFAPSATFVLA
                     EPLTLTVGTSFQRFQTQFPAARTESANAVTTTLRLARRLEDSRGDKHDVEAGYSLRAA
                     TKILDTDFAYARHQVEAGYTFTRDAHRLGARFEAGRISGTAPLFERFVLGNTRTLRGW
                     HKFAVNPLGGTREAHGSIEYGYHWFQVFYDTGALWDRGEDAEAKHAIGMGIRTKGGFF
                     LAMAFPVKSGRATPLFMAGTEF"
     gene            5036..7171
                     /locus_tag="VLE22_03410"
     CDS             5036..7171
                     /locus_tag="VLE22_03410"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HSB13479.1"
                     /translation="MSHTRIIPLLLAATALSAEVVHDKASQTWTLRSAGVEYALALKD
                     GAVTLAYFGPTGSAPWKPASGRPAAPRYDLAGRADGRSLAADAFELTGHEVHSLRAGV
                     DELLLLFRHRRLPLALEARYAAWGDTGVITRRVALTNLGTQPVRVESLASLSWSLPPG
                     GYELTYLHGGWGRERQLATEKLGAGQRVFENRRGRSTSGYSPWFCLRNETTGVRYIAQ
                     LAWSGNWEMLFERYADSMPLHDQDLRAELGLRFDFGGPLGLEPGRTFDFPRVAFSASM
                     GDLDDASNNLHRYQRTYVFPRIASNDPPLVQFNSWYPFPGKLSVTDLKRCADVAAELG
                     AEVFVLDAGWFNKKDWSRELGDWQVDGTAFPRGLQELSEYVRKKGMKFGIWVEIENLG
                     VESNTFTQHPEWCLSYEGKPIQTSARYHLNFALPEVRQWARAVIDRLVRDYGIEWVKI
                     DYNIDIGEQFDPPGAGRSGDVLYRHITAYYGWLDDVRAAHPKLIVENCSSGGLRFDAG
                     ILEHTHTTWLSDVVRPLPSLQLGYGCTVEFAPQACNHWMVGDKDNGEVDNSRPPGWWD
                     FLFRVPMNGQLGISSRVFDWTPALKQRAAENVALYKQIRSTISAGDAFHLAPQPDHDN
                     PAGWMAIQYSVPDGARSVLLAYRLAEGQPTQTFKLRALRRDASYRVKRDGMAQSGRSG
                     AELMDTGLPVELPAEWRATVFELDAGPSF"
     gene            7214..8059
                     /locus_tag="VLE22_03415"
     CDS             7214..8059
                     /locus_tag="VLE22_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13480.1"
                     /translation="MPSKSKPLLTRRRLLRQSAAAGVASVLNNAGAAVVDLHPKGNFR
                     YLRGIAPYSSGALAERNHQVVHVTFASPPPYRLGFLAIDSHLKQEKRPPESLCGIELR
                     SPRPFTFDGFNQFNQEYIEQLKQRGLFTDGTNPVARTNVAPELNPPSEVLLYGFSYTA
                     PAKTTKQTFVIAGAGELLGERLDARDIVKRGDVSPAGLRAKATHVLGLMDQRLRTLGV
                     SWGDVTIVNVYTVHNIAPLMKDLLLARAGAATLHGIDWHFAHPPIREIEFEMDVRGCA
                     RELVL"
     gene            8099..9802
                     /locus_tag="VLE22_03420"
     CDS             8099..9802
                     /locus_tag="VLE22_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB13481.1"
                     /translation="MILNARRRPLCALPLLCSVLLGQPQKPTEPPAVVIRSTTRLVQV
                     NVVVRDRNGNAVTGLTKDDFVLEDDGKPQDIRVFAFQPSEKPARPTARPAPGVSSNRY
                     VERAAVGVTAILLDGLNTSWGDQAATRQQMVRYLQNVRPEDRIALYVLGRELRILHDY
                     TSEPSALLKVLSAYTGRHSPELDASHQPMIEEIAQSVAAEYGNTKEARLAMEALQGVI
                     NTLKEEEFFYKEQRVLRTFKALEAIANHLAGVPGRKNLIWISAAFPLQLGYFESPDAA
                     RGPSPESSDSPFSARYPGRRSTPADRSSLLVKHGRSRTFGDEFQRAARALSTSNLSVY
                     PIDARGLSPNPRAFENIATMNDLAHYTGGKAYRNTNDIMGSIREAVQDAEVSYTLAYY
                     PNQSNLDGRFRRIKVKVRHPSVSLRHRLGYYDLAEPSPGEASDAALRDALWSPMDATA
                     VLMDARLKRAEAAQAFALEVTIDPSGITFQPKNDRWTADLEFFLAQTGEEGREHGITS
                     QTVHLELLPEIYENVRRDGYILRQSVNRNPGATSLRVLVRDVLSGSIGRVIVPFEAPP
                     L"
     gene            9899..10294
                     /locus_tag="VLE22_03425"
     CDS             9899..10294
                     /locus_tag="VLE22_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13482.1"
                     /translation="MQRTILGALALAAAMPFAFDARERYNDYRPYGYQDPVSRAIRNL
                     EGIGSRSFVDRHEQRHFRGALEELYRFQQRARDGRFDKGRLDRAIEHIDHLAGADQLH
                     PRARQILRGDLWELRRFRSEYSRDRYRGW"
     gene            complement(10318..>11344)
                     /locus_tag="VLE22_03430"
     CDS             complement(10318..>11344)
                     /locus_tag="VLE22_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13483.1"
                     /translation="PGHGLREGASIAAAPPSDLTGRLQSGIRVDMRRAFVAVPAMTLV
                     IQICTGGLLVGDPGSQNDQKKSWTVGIYTGPSPFQLSAPANVKNPVLTGAAVTDLNVD
                     TLAHPFMVVTKSRYYLFFTAKDLKTDKGGIGLAESKNGREWKFRRTVVHEPFVLSHPY
                     VFQWNNDYYMIPEGHTESSVRLYKATKFPDEWKYEGDILQGDHFISPTLVRYKDMWWL
                     FTVRSGNKTLRLFYARDFKGPWTEHPLSPIVKDDLNTARPAGRPFVIDGVLYRLAQDC
                     YPTYGNQVLAFRITDISPTTYSETMIDTPIVKASSQGWNSDAMHHVDAHQIGKNKWIA
                     AVDALGN"
BASE COUNT         2086 a         4072 c         3138 g         2048 t
ORIGIN      
        1 tgtgatgact ggaccctgcg ccgccgcaaa cgcccccgcc aacagggaaa gcaccgcaac
       61 cacaccatac tttcgcatgg cctgatccct ctggccttac tgtagcatca gataatcaga
      121 cccgtatagc tctatgtttc gcctttattc ccgcccgttc gcggcccgag gatcacgctc
      181 gcgactcaaa ccgagcgccc gtatcctctt atggaggttc gtccgctcca tccccagcgc
      241 ccgggccgcc gccgacacgt tccactccgc ctcgctcaac gccttcacca ggtgctcccg
      301 ctcggccgtt tcgcgcgcct gctgcaggct cgacggcgtt ccctcccgcc gcgccgcgcg
      361 cacctccgcc ggaatcgagt cttcgcccaa ttgctcgcct gccgacagta tcgccatccg
      421 ctccacggtg ttacgcagtt cgcggacgtt gcccggccac ccgtaccgct cgaacacagg
      481 aaaaacttcg tccacgaatg ccttcgcccg gaaattgttc cgagcgcaga actcgccgag
      541 gaaatactcc gccagtggcc gcacgtcctg cgggcgctcg cgcaaggccg gcacccgcac
      601 cggcacaacg ctcagccggt agtaaagatc ctcccggaac tgctgcttcg ccaccagcgc
      661 cgccaggtcg cggttcgtcg ccgagatcac ccgcaccgtc acgcggatcg tctgctcgcc
      721 gcccacacga tgaaattcgc cctcctgcaa cacacgcaac agtttcgcct gcgatgcagg
      781 atgcaggtcg ccgacctcat caaggaatag cgtcccgcta tccgccaact caaatttccc
      841 ccgcctcgcc gacgccgctc cagtaaacgc gcccttctca tgcccgaaca gttcgctttc
      901 cagcagttcc gtcgggatcg ccgcacagtt cactttcaca aatggcccgg gagcaaacgg
      961 gcttaagcgg tgaatctgct ccgccagcag ttccttaccc gtccccgact cgccgatcaa
     1021 cagcaccctg gcgtccgagc gcgccgccat cgtcgcctgc tccaccgccc tctgccacgc
     1081 cgggctccca ccgatcatcc tcggaccgtc gcccacgagt tcccgtagcc gcgcgttctc
     1141 ctgccgcagg ttcgcttgtt ccagagcgtt cttcactgcc agcagcacgc ggtcccgtcc
     1201 cagcggtttt tcgaggaaat cgaacgcacc ggcgcgagtc gcttccaccg cgtccgcaat
     1261 cgtggcgtgt ccggagatca tcactgccgg cgcacagtgg tccgtccctc gcaggtcgcg
     1321 tagcaggtca ataccgttgc cgtccggcag cttcacgtcc agcagaaaga cgtccgcgcg
     1381 ccccgtgtcc ggatgcgcgc ggcagtcgga aatcgagcgg cacacagtca ccgcgtaccc
     1441 ttcgcgctcc aggatcagcc ggagcgaacg gccgatgttc tcttcatcgt caacgatcag
     1501 gattctgttc cgcggcatat cgcggcagca cgacgcggaa cgccgcgccg cccagttctc
     1561 ctttcaccag caaaatatca ccgcccgcga gcagggcgct cttgcgggcg atcgacaagc
     1621 ccaatcccat tccacctttc ttgaaggaaa tggccggctc gaacagcgac cgccgcacat
     1681 cctcgctcaa ccccggaccc gagtcgtgca cctccaccgc caccttccca ttcagcagcg
     1741 acgtccgccc caacacctca ccgcccgctc ccgccgcctc ggcagcgttc tctagcaagt
     1801 tcgtcagcac acccttcacc agatcctcgt ccgtatgcgc cgccgggagt ctcggatcga
     1861 gccgcagcgt gtaccgcacc tccggccgtc cggtcctgag aaacgccacg cgttcttcga
     1921 caaccgcgtt gacatccagg acaccgggcc gcaccggagg ctcggcggcg aactcggaaa
     1981 acgcccgcac gcgccgctcg agtgtttcca cttcctcgcc gacgatctga gccgcctgct
     2041 ggataaatgc gggatcgctt tcgccggcac gtgccagcac ctcttccata gtcagccgga
     2101 tcggcgtgag cgaattcttt agctcgtgcg ccatcttgcg cgccagcgcc tgccagcttg
     2161 caagctgcgc caggtagacg agccgcgccc ggttctgctc cagttcctcg gctgtgcggt
     2221 taaaggcgtg catcgcctcc ccgatctcgt cttcgcggtc cacggatacc cgcaccgcca
     2281 ggttgcccga cgccagttcc gaaagcgccg ccgagagctc gcgaatcggc cggctgacgc
     2341 gagacgccag gtaagccagc agcgcaaatc cgaccaacca cacactcaga gccagcagca
     2401 gcagagtcag cgtgaatccc cggcgcaggt ttcgctggtt gccggcgtcc accagcgccc
     2461 tggcctgccg gtattgcctc gtcaagctct ccatcccgac gctgccgagc gggcgtgacc
     2521 acacccgcac ctcaccccca cgccgggtca tgaagttcag ccggtcgcca ttcgtacccg
     2581 aaagaacgaa gcgctctgtc tccccgctct cctgaaacgc acgaacctcc gctggccagc
     2641 ttgcctccgc gcccgggaga taccgctgcg gttcgatagc gccttgcgcg gcgtcgcggc
     2701 gcagcgagtc ctgcgcctgc cggtaatact cgcgtcccag ccgttgaagc gcacgggaga
     2761 tttcgtcgag ttcgcgagtt gaggcgtagg tcaggctcca ctcaagcagc gacgacgtaa
     2821 accacagcgt cgccccgagc gggggaatcg tcgcgacgac aaacagcagg atcagcctgc
     2881 cgcggagcga tcgaggcatc cgggaagtca ccccgccgct acttcagttc cgccagccgg
     2941 aacggtcctc cttcggccag ccatcgcgac tcgccgggat ggggagagcg gctgaagaat
     3001 tccaccatgc gcgcgaggtt gatgccagcg ttttcctccg gaggcgtgtc gccattgggt
     3061 tcctccgcgc gcacctccaa tctaacccaa aagggcgacg agggagataa ccgcgcgcgc
     3121 gacactgaga cgttatccag gcaccacgcc tccgccgcgc ctgcggcgag gtgcgaagtc
     3181 gctttccgcg tgccgcccag ccgcgtcacg gcgaactttt cttcccagag gtcgaagctc
     3241 atcgcgaagc gcccgcccgt ccgatcgatc accgttgttt tccgatcccc aagcagggtg
     3301 agctgaacgg caaagatcac ggaattcccg ttgcgtagct gttcgagcgg ccggccctcc
     3361 acaaaatgga agttcggcgc ggccaccctc agcgttcccg tgccaagacg cacctcaagt
     3421 ccttggccgt acagcgcctg cgccgccgcc agcagcgcga ggtgacctgc cgtcgcaagc
     3481 ttcatcggaa ttcgccaacg acccattatt gtcaaacagc gcccgggaat cgggagagaa
     3541 ttcggtgacc gtcccggctc ccgttaaacg gtcctctcgg tcatcgaggc ccgtgggagt
     3601 gccatcgaat tccctcccgt cccccagccg caaaacggct aaaattccgt ccccgccata
     3661 aacaacggcg ttgcccgccc cgacttcacc gggaacgcca tggccaggaa gaaccctcct
     3721 ttcgtgcgaa ttcccatgcc gatcgcgtgc ttcgcctcgg cgtcctcgcc acggtcccaa
     3781 agagcacccg tgtcatagaa gacttgaaac cagtgatacc cgtactcgat cgagccgtgc
     3841 gcctctcgcg taccacccag tggattcact gcgaacttgt gccagccacg caaggtccgt
     3901 gtgttcccca ggacgaagcg ctcaaacagc ggcgccgtgc cagagatgcg ccccgcctcg
     3961 aatcgcgcac ccaacctgtg ggcgtccctc gtgaaggtat agcccgcctc cacctggtgt
     4021 cgtgcataag cgaaatcggt gtcaagaatc ttggtggccg cgcgcaggga gtaccctgct
     4081 tcgacatcgt gcttgtcgcc acgcgaatcc tccagccgcc gcgccaggcg cagagttgtt
     4141 gtcacggcgt tggcagactc ggtccgcgcg gccggaaatt gcgtctgaaa gcgctggaag
     4201 gaggtcccca cggtgagtgt gagcggttcc gcgagcacga acgtcgccga cggtgcgaag
     4261 tcctgccgtg tacggtacac acccgggaca tccgaccggg cggcaagagc cgcttgggta
     4321 tcggcgttcc acttttggtg gtagctcgcg aatcggaacc cgagccggac gcgttccgtc
     4381 ccaactttcg tgttctcgta gcccgctcgc acgcccgcgt accgctcgag caattcgtcg
     4441 ccatcgctta cgatgccgag gctgaaccgg ttcgtaccca cggtcgtggt cgtcccgatc
     4501 gcaccactcc agccctgctt cgcgtggtag acgccccgcg tcatggacgc gtcgaagttc
     4561 tgtcgcggcc gtctgcccgt cacttcgaac agcacgatca cttgctccgg ttgcgtgccg
     4621 cgctcgagtt tctgctgcac cagacgggcg ttcagctctc tgcggatccg ctgtgcgagc
     4681 cggtccagct tctgtggatt cagcttctcc ccaaccagcc ggtccaagtc ttcccgcaat
     4741 gtccggctga tgcggttgat cccgttgtcc gagatatcca cccgctcaac ccggtaccgc
     4801 gagttcacgt tgacctgtga ccccgcatct gaggcgccca acccggacgc catcacaagc
     4861 gcgatcagcc agccgtactt catacgcccc tcaatgtgca cgcgccttgc cacacgtaac
     4921 ccgttgaatt acaaagacgt gtgacgcaca aaaccgtgag ccggtttcac aggtgtgcgt
     4981 cgggaagcca tccgcgccac aatgggggac ttacatccaa ttttcggagc aatctatgtc
     5041 ccacacacgg attattcctc tcctcttggc cgccaccgcc ctatctgccg aagtcgttca
     5101 cgacaaagct tcgcagacct ggaccctccg cagcgccggc gtggagtacg cgctcgcgct
     5161 gaaggacggc gctgttacgc tcgcatactt cgggcccacc ggctcggcgc cctggaaacc
     5221 agcctcaggc cgacccgcag ccccgcgcta tgacctcgct ggccgtgccg acggacggag
     5281 cctcgcggca gacgctttcg agctcaccgg ccacgaagtc cactcgctgc gcgcaggtgt
     5341 cgacgaactt ttgctcctct tccgccaccg ccgcctcccc cttgcactgg aagcgcgcta
     5401 cgctgcgtgg ggcgacaccg gtgttatcac acgccgcgtc gcactaacaa atctcgggac
     5461 gcagccggtc cgcgtcgagt cgctcgcgtc cctctcctgg agcctcccgc ctggcggcta
     5521 cgagctgacg tacctccacg gcggctgggg ccgtgaacgc cagcttgcca ctgagaagct
     5581 cggcgccggc cagcgcgtct tcgagaaccg tcgcggccgc tccaccagcg ggtactcccc
     5641 ctggttctgc ctccgcaacg aaaccaccgg cgtccgctac atcgcccagc tcgcctggtc
     5701 tggcaactgg gagatgctct ttgaacgcta cgccgacagt atgcccctcc acgaccagga
     5761 tctccgcgca gaactcggcc tccgattcga cttcggcggc ccgctcgggc tggaacccgg
     5821 acgcacgttc gacttccccc gggtcgcctt cagcgcctcc atgggcgacc tcgacgacgc
     5881 ctccaacaac ctccaccgct accagcgcac ttacgtcttc ccgcgcatcg cctccaacga
     5941 cccgcctctt gttcagttca actcctggta cccgttcccc ggcaagctct ccgtcaccga
     6001 cctgaaacgc tgcgccgacg tcgcggctga gctcggggcc gaggtctttg tcctcgacgc
     6061 gggctggttc aacaagaagg actggagccg cgaactcggc gactggcagg tcgatggcac
     6121 cgcgttcccg cgggggcttc aggagctctc cgaatacgtc cggaagaaag gcatgaagtt
     6181 cggcatctgg gtcgaaatcg aaaacctcgg cgttgaatcg aacaccttca cccagcaccc
     6241 cgagtggtgc ctctcctacg agggcaagcc catacagacc tccgcgcgct atcacctgaa
     6301 ctttgcgctg cccgaagtcc gccagtgggc ccgcgccgtc atcgaccgcc tcgtccgcga
     6361 ctacggcatc gagtgggtaa agatcgacta caacatcgac atcggcgagc agttcgatcc
     6421 gcccggtgca ggtcgcagtg gcgacgtact ctatcgccac atcaccgcct actacggctg
     6481 gctcgacgac gtccgcgccg cgcatccaaa gctcatcgtc gaaaactgct cgagcggcgg
     6541 tttgcgcttt gacgccggga tcctcgagca cacccacaca acgtggcttt ccgatgtcgt
     6601 caggcccctc ccgtcgctcc agttgggcta cggttgtacc gtcgagttcg caccgcaggc
     6661 ctgcaaccac tggatggtcg gcgacaagga caacggtgag gtggacaatt cgcgcccgcc
     6721 tggctggtgg gactttctgt tccgagtccc gatgaacggc cagttaggta tctcgagccg
     6781 cgtattcgac tggacaccgg cactgaagca gcgcgcggcc gaaaacgtag ctctttacaa
     6841 gcagatccgt tccaccattt cggcgggcga cgccttccac ctggcgccgc agcccgatca
     6901 cgacaacccc gccggctgga tggcgatcca gtacagcgtc cccgacggcg cgcgcagcgt
     6961 cctgctcgcg taccggctcg ccgaaggcca acccacgcag accttcaagc tccgcgctct
     7021 gcgccgcgac gcctcgtatc gcgtcaaacg cgatggaatg gcccagtccg ggcgttccgg
     7081 cgccgaactg atggacaccg gcctgccggt cgaactaccc gccgaatggc gcgccaccgt
     7141 cttcgagctg gacgccggac cgtcattctg aacgccgctc ccggctcttg tacactacct
     7201 gtgggtgtct cttatgccgt ctaagtctaa gccgttgcta acacgtcgga gactgctccg
     7261 ccaaagcgcc gccgccggag ttgccagcgt tcttaacaac gcgggcgccg cagtcgtcga
     7321 ccttcatccg aaaggcaact tccgatacct gcggggtatc gcaccgtact catccggcgc
     7381 tctagcagag cggaatcacc aggttgtgca cgtcactttc gcctcgccgc cgccttaccg
     7441 tctcggcttc cttgcgatcg acagccacct caagcaagag aagcgtcctc ccgagtcgct
     7501 gtgcggaatc gaactgcgtt cgccgcgacc ttttacgttc gacggcttca accagttcaa
     7561 tcaggaatac atcgagcaac tgaagcaacg cggcctcttc accgacggca caaatccggt
     7621 agcccgaacg aatgtagcgc cagagctgaa cccaccctcc gaggtgcttc tctacgggtt
     7681 cagctacacc gccccggcga agaccacgaa acagactttc gtcatcgccg gcgccggcga
     7741 gcttttgggt gaacgtctcg acgcccgcga tatcgtcaag cgtggcgatg tttccccggc
     7801 tggcctgcgt gccaaggcca cgcacgttct cggcctcatg gaccagcgcc tccgcaccct
     7861 cggcgtctca tggggagacg tcaccatcgt caacgtctac actgtgcaca acatcgctcc
     7921 gttgatgaaa gacctgttgc ttgcccgcgc cggcgctgcc accctccacg ggatcgactg
     7981 gcacttcgcc caccctccca tccgggaaat tgagttcgaa atggacgtcc gaggctgcgc
     8041 ccgcgaactg gtgctctaag ctcgtccacc gcgcgataat gtaaggcgga caatcgtgat
     8101 gatcctcaac gcacggaggc gcccgctgtg cgcgcttccg ctgctctgct ccgtcctgct
     8161 tggacaacct cagaaaccca cggagcctcc cgccgttgtg atccggtcta ccacgcgcct
     8221 cgtccaggtc aacgtcgtgg tccgcgatcg gaacggaaac gccgtcaccg gcctcaccaa
     8281 ggacgacttt gttctcgaag acgatggcaa gccgcaggac atccgtgtgt ttgccttcca
     8341 gccgtccgag aagcctgcac gtccaaccgc gagacccgcc cccggagtca gttccaatcg
     8401 gtacgtcgaa cgcgccgccg tcggtgtcac cgccatcctc ctcgacggcc tcaacacaag
     8461 ttggggagac caagcggcta cgcggcaaca gatggtccgc tatctgcaaa acgtccgccc
     8521 ggaggaccgc atcgcgctct acgtcctcgg acgggagctt cgcatcctcc acgactacac
     8581 gagtgagccg tcggcattgc tcaaggtgct ctctgcctat accggtcgcc attcacctga
     8641 actcgacgcc tcccaccagc cgatgattga ggagatcgcc cagtccgtcg cagcggagta
     8701 cgggaatacc aaagaggctc gactagccat ggaagcgctg caaggggtca tcaacacgct
     8761 caaggaggaa gagttcttct acaaggagca gcgcgtcctg cgcaccttca aggcgctcga
     8821 agccatcgcc aatcatctcg ccggcgtgcc cggacgcaag aacctgatct ggatttccgc
     8881 ggccttcccg ctccagctgg gttacttcga gtccccggat gccgcgcgtg gcccctcgcc
     8941 ggaaagctcc gactcgccat tcagcgctag gtaccccgga agacgcagta cacccgccga
     9001 ccgaagctcc cttctcgtca agcacggccg ctcccgcacg tttggagacg aattccagcg
     9061 agctgcccgc gccctgagca cctccaacct ctcggtgtac cccatcgacg cccgcggcct
     9121 gagtccgaat cccagggcgt tcgagaacat cgccaccatg aacgaccttg ctcactacac
     9181 cggtggcaag gcctaccgca acaccaacga catcatggga tccatccgcg aggccgtcca
     9241 ggacgcggaa gtctcctaca ctctcgctta ctacccgaac cagtcgaacc tcgacggccg
     9301 cttccgccgc atcaaagtga aggtgcgcca ccccagcgtt tccctgcgcc atcgacttgg
     9361 ctattacgac cttgccgagc cctcgcctgg agaggcctct gacgccgcac ttcgtgacgc
     9421 gctctggagc ccgatggatg ccaccgccgt tctgatggac gcgcggctca agcgggccga
     9481 agcagcccaa gcattcgctc tcgaagtaac gatcgatccc tccggcatta cgttccagcc
     9541 gaagaacgac cgctggactg cggacctgga gttctttctc gcccaaaccg gcgaagaggg
     9601 ccgggagcac ggcatcacgt cccaaacggt ccacctggaa ctgctccccg agatctatga
     9661 gaatgtgcgc cgggacggct acattctccg tcaatccgtg aatcgaaacc ctggcgccac
     9721 ctcactgcgt gttctcgtcc gcgacgtcct gtccggtagc atcggtcgcg tgatcgttcc
     9781 gttcgaagca ccaccactct gacgtcgcgc aggtcaaaag gatgtcgcgg tccagccggt
     9841 tgatttttcg taaccagatg cgcactgagc cgcgtctatc gggcgggagg taacggcgat
     9901 gcaacgaacc atcctcggcg ccctagcgtt ggccgccgcg atgcccttcg cgttcgacgc
     9961 ccgagaacgc tacaacgact acaggcctta cggctaccaa gaccctgtaa gccgcgccat
    10021 ccgcaatctg gagggaatcg gctcacgatc gttcgtggac cgccacgaac agaggcattt
    10081 ccgcggcgcg ctcgaggaac tctaccgatt ccagcagcgc gcacgggacg gccgcttcga
    10141 caagggccgc ctcgacaggg ccatcgagca catcgaccac cttgccggtg cggaccagct
    10201 acatccccgc gcccgccaga tcttgcgcgg cgatctctgg gagctgcggc gctttcgctc
    10261 ggaatactct cgcgaccgat accggggttg gtaaggactg ccccgccaag cgccaagcta
    10321 gttgcccagt gcgtcaacgg ccgcgatcca cttgttcttt ccgatctggt gagcgtcgac
    10381 atggtgcatg gcatcggaat tccagccttg cgaagacgct tttacgatcg gagtgtcgat
    10441 catcgtttcc gagtaagtcg tggggctgat gtccgtgatg cgaaacgcaa gtacttggtt
    10501 cccgtacgtc gggtaacaat cctgagcaag ccggtatagg accccgtcga tgacgaacgg
    10561 ccggcccgcg ggcctggcgg tgttcaggtc gtcctttacg attgggctta gcggatgctc
    10621 cgtccaaggc cccttgaagt cccgtgcata gaaaagccgc agcgtcttgt tcccggagcg
    10681 caccgtgaac agccaccaca tgtccttgta ccggaccagc gtcgggctga tgaagtgatc
    10741 tccctgcaat atgtcgcctt cgtacttcca ttcatccggg aacttcgtcg ctttgtagag
    10801 ccgcaccgag gactcggtgt gcccctcggg aatcatgtag tagtcgttgt tccactgaaa
    10861 cacataaggg tgagacagaa caaaaggctc gtgcacgacc gttcgccgga acttccactc
    10921 gcggccgttt ttgctctccg ccagaccgat accaccttta tcggtcttca ggtccttggc
    10981 cgtaaagaac aggtaatacc gggatttggt gacgaccata aatggatggg cgagtgtatc
    11041 cacattcagg tcggtgacag ccgcgcccgt cagcacgggg ttcttgacat tcgctggcgc
    11101 ggaaagctga aacggcgacg gcccggtgta gatgccgacc gtccaagact tcttctgatc
    11161 gttctgtgac ccgggatcgc ccacgagaag tccgccggtg cagatctgta tcaccagcgt
    11221 cattgctggt acagcgacaa atgctcgtct catgtcaact ctaataccgg actgaagccg
    11281 ccccgtcaag tcgctcggcg gcgccgctgc tatactggcc ccttcccgga gcccgtgccc
    11341 gggg
//
LOCUS       DATIFQ010000079         4854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_25149, whole genome shotgun sequence.
ACCESSION   DATIFQ010000079 DATIFQ010000000
VERSION     DATIFQ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25149"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VLE22_03435"
     CDS             complement(<1..1578)
                     /locus_tag="VLE22_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HSB13484.1"
                     /translation="MEGLHHLELVILLLALVLALTTVAQKILIPYPILLVIGGLALSV
                     IPGLPVVTLSPDLVFLVFLPPILWAAAYFTSWREFRANLRPISLLAVGLVLATTGAVA
                     AVAHAMLPGIGWAAAIALGAIVSPPDAVSATAIGRRLHIPRRIVTILEGESLVNDATA
                     LVLYRAAVGVAIGGSYVMGQTVIQFVFAASVGVAVGLAVGVAVRWALCATEDSFTQTA
                     ITLLAPYVAWVLGELTQASAVLACVAGGLYIRRHFSAAVAPTTRLQARAVWNLLIFVL
                     NGFIFILIGLQLAVLRSALPSARFGQVLMAGALVSGTTILVRLVWVPLAAILPRALSP
                     SLRARDPMPPWPALFLIGWTGMRGIVTLAAALALPLTTAAGQPFPFRAEIILISFTVI
                     LATLVLQGLSLAPLIRAINLQEDHDQAQEERQAREHAATVALRRLDELAGEDWPPAEH
                     VDRLRVHYSRQLERFASSGSKDQNRAFETGESFRRLRHETLSAERLALIDLRNDGTIS
                     DELLHRLEHELDVEALRL"
     gene            1857..2129
                     /locus_tag="VLE22_03440"
     CDS             1857..2129
                     /locus_tag="VLE22_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13485.1"
                     /translation="MDSSLANLLTGLVSGAVGGNIAGALMKNLSLGLVGNTLAGIVGG
                     GLGQQVLSALGLLQNLGIVGDVGGSAVGGGAVMAVVALIKNAMSKS"
     gene            complement(2296..4242)
                     /locus_tag="VLE22_03445"
     CDS             complement(2296..4242)
                     /locus_tag="VLE22_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HSB13486.1"
                     /translation="MLSRTAASCFWIARYTERAEYTARLINVHYHLLLESADLQDQIG
                     IWRWYLESTGQLSLYEERAQPLATPLVMEFLTLDKGNPNALVNLISAARENARGIQDQ
                     LSSEVWHHINRVHLAWRDYTPASIAASPHRILTEVQDTCYTLHGIMASTMLHDEGWTF
                     YRLGKDIERASHTARLLVHPILLQTSREPTELSDLHQCIAILKSASAYQAYRKVSRSV
                     VLPEKIVEFFLLNQYFPRSVRFCARALRQNIERIAEHPLGPSSREPARLVGQMAADLD
                     FITLDEIHEIGLENFLTEVVRQLDRITTAISRTYFRAGETPEVTISSPRRHLRLEHHE
                     TAVHNIKALLSARHQFTYRYESPVTNVRTIMRVAPHQHYGRQRRLDIRWHMDPPADYR
                     HYTDAFGNLVWQMDHARVEQEITCTVEMRVETEAGYLTDGTLALQGTDPQATDCTVDS
                     SEFMRLTHLVDRSEKLARLAERLRGGGGASGRLAEAILHHVHAALRYERGRTHVGTPA
                     SEAMALGSGVCQDYTHVMLAICRLAGLPARYVSGLLPGEGQMHAWVEVLLPGTVQKPP
                     TWVGYDPTHQRRCDERYITVAVGRDYQDIAPTSGYYEGEAKNQLEMAVSVVLESHGPA
                     DRWLSGQGLALPPVSVPDEHQQQQ"
     gene            complement(4249..>4854)
                     /locus_tag="VLE22_03450"
     CDS             complement(4249..>4854)
                     /locus_tag="VLE22_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HSB13487.1"
                     /translation="FLDPEVFRPDSVLGVPGLVRAYHAGNVALANAIGNGVADDKAIY
                     AYVPKMIAFYLSESPILPNVPTYLCSVEDDRRYVLEHLPSLVVKAVNESGGYGMLMGP
                     ASTKAEQEEFRARILAHPRNYIAQPIVSLSRLPCMVDDPDGGFRLAGRHIDLRPFVLC
                     GQDITLSLGGLTRVALREGSLVVNSSQGGGSKGTWVLYGED"
BASE COUNT         1002 a         1513 c         1476 g          863 t
ORIGIN      
        1 gagacgcaac gcttcgacat cgagctcgtg ctccaggcga tgcaacagtt catcgctgat
       61 cgttccgtca ttgcgcagat cgatcagcgc caggcgctca gccgataagg tctcgtggcg
      121 caaccgtcga aaggactccc ctgtttcaaa agcacgattc tgatccttcg aaccggagct
      181 cgcaaatcgt tcaagttgcc ggctgtaatg aacgcgtagg cgatcgacat gctccgctgg
      241 cggccagtct tcacctgcca gttcatccaa ccgtcgcaag gcaaccgtgg ccgcatgttc
      301 ccgcgcctgt cgttcctcct gcgcctggtc gtgatcttct tgcaggttga tggcccggat
      361 caacggcgca agtgaaagtc cctgcagcac cagggtggcc agaatcactg taaagctgat
      421 caggatgatc tcggctcgaa agggaaaggg ctgtccggca gccgtcgtga gcggtaatgc
      481 cagggcggcc gccaacgtca cgatcccgcg catcccggtc cagccgatca aaaagagcgc
      541 aggccatggc ggcatgggat cccgggcccg caacgacgga ctcagcgccc ggggaagaat
      601 cgccgccagg ggcacccaga ctagccggac gagaatggtc gtgccgctca ccagcgcgcc
      661 ggccatcagt acctgcccga accgggcgga cggcagcgcc gaacgtaaga ccgcaagctg
      721 gaggccgatc agaataaaaa tgaagccgtt gagcacgaag atcaggagat tccacacggc
      781 acgagcctgc agacgcgtgg tcggcgccac ggccgcacta aaatgccgcc gtatgtacaa
      841 acctcccgcg acacaggcga gcacggcgga cgcttgcgtc aattcaccca gcacccatgc
      901 cacatagggc gccagcaagg tgatcgcggt ctgcgtaaag ctatcttccg tggcacagag
      961 tgcccagcgc accgccacgc ccacggcaag accgacagcc acccccacgg aggctgcaaa
     1021 gacaaactgg atcaccgtct gccccatgac atagcttccg ccgatcgcga cgccgacagc
     1081 ggcgcggtac agcacaagcg ccgtggcatc attgaccaga ctttctcctt cgaggatcgt
     1141 aacgatacgg cggggaatgt gcagacgtcg gccgattgcc gtcgccgaca cggcatccgg
     1201 cggagagaca atggcgccca gcgcaatcgc cgcagcccat ccgataccgg gcagcatcgc
     1261 atgcgccacc gccgcaaccg ctcctgttgt cgccagcacg agcccgaccg ccagcaagga
     1321 gatcggcctg agattggcgc gaaattcacg ccacgacgtg aaataggcgg cggcccacag
     1381 gatgggcggc agaaagacga ggaacaccag atcgggactg agcgtgacca ccggaagccc
     1441 cgggatgaca ctcagagcca atccgccgat cacgagaaga atgggatagg gaatgaggat
     1501 cttttgggca accgtcgtca gcgccagcac cagcgcgagc aggagaatga cgagttcgag
     1561 atgatgcaga ccctccatcc ctcacctttc ttcacgatgc atgaccgttg agagcggaaa
     1621 tttcgagaca ggctacttgg gggagaaaac ggagtcaact atgggaaggt gacattgacc
     1681 aggcactcat gcactcgccg cacgcggacg gggcgggatg ctttattgga atgttttttg
     1741 acccggatgt gaccgacggc gtgtcatcac ctgtttactt cggcctctgt cctgactaca
     1801 ctggcctcgc tcgtacctca gagcgccgca acgatccctc acctcaagga ggatctatgg
     1861 acagctcgct cgcgaatcta ctcaccggat tagtcagcgg tgcggtcggc gggaacatcg
     1921 ccggggccct gatgaagaac ttgagtctcg gcctcgtcgg gaacaccctc gccggaatcg
     1981 tcggcggcgg cctcggacaa caagtcctca gcgcactggg gctccttcaa aacctcggga
     2041 tcgtaggcga cgtgggcggg tcggcggtcg gcggcggagc agtgatggcc gtggtcgcgt
     2101 tgatcaagaa cgccatgtcc aagtcgtaag tctgctcttc tcagcggcca cagtcctgtc
     2161 actcagtgga gacaatgtgt gcgtaccgca cctccgttgg gcgcctgctc acacatactg
     2221 tccgccgcgc gcggacgaca ggatcgaaaa gtaaaatgtc cccgcattgc cggtcaagat
     2281 tcccctcctc gaccgttact gctgctgttg atgttcatcc ggcacggata cgggagggag
     2341 tgcaaggccc tggccgctga gccagcggtc ggcgggtccg tgggattcca gtacgacgga
     2401 cacagccatc tcgagctgat tcttggcttc gccttcgtaa tacccgctgg tcggggcgat
     2461 atcctgatag tctcgtccca cagccacggt gatgtagcgc tcatcgcacc gtcgttggtg
     2521 agtcggatcg taaccgaccc aggtcggggg tttctggacc gtgcccggca ataagacttc
     2581 aacccacgcg tgcatctgcc cttcgccggg gagcagcccc gacacatagc gcgcgggcag
     2641 gcccgccaat cgacagatgg ccagcatgac atgcgtgtag tcctggcaaa cccccgaacc
     2701 cagggccatg gcttcggaag caggagtacc cacatgcgtc cgcccgcgct cgtaccgcaa
     2761 ggccgcgtgc acgtggtgca gaatggcttc ggcgagcctc cccgacgcgc cgcctccgcc
     2821 gcgcagacgt tccgcgagac gggcgagctt ctcggaccga tccacgagat gggtgagtct
     2881 catgaattcg gacgagtcca ccgtgcaatc agttgcctga ggatcggtgc cttgcagcgc
     2941 gagtgtcccg tccgtcagat acccggcctc ggtttcgacg cgcatctcca ccgtgcaggt
     3001 gatctcctgt tccacgcggg catgatccat ctgccagacc agattgccga aggcatcggt
     3061 gtaatgccgg taatcggcgg gcgggtccat atgccaccgg atatcgagac gccgctgtcg
     3121 cccgtagtgc tggtgcgggg ccacgcgcat gatcgtgcgg acattcgtca ccggcgactc
     3181 gtagcgatag gtaaactgat gccgtgccga gaggagcgcc ttaatgttat ggaccgccgt
     3241 ttcatgatgt tccaatcgca gatgccgccg cgggctggag atcgtgacct cgggggtctc
     3301 cccggccctg aaataggtgc gtgaaatcgc cgtggtaatc cggtccagct gccgaacaac
     3361 ctccgtcaag aaattttcca gacctatttc atggatttca tccagcgtaa tgaaatcaag
     3421 gtcggccgcc atctgcccca ctagccgggc aggctcccgg ctggaaggcc ccagcgggtg
     3481 ttccgcaatc cgctcgatat tctggcgcag cgcccgcgcg cagaaccgga cggacctggg
     3541 gaaatactga ttcagcagaa agaactccac gattttctcc ggcagcacga cagagcggga
     3601 caccttccgg taggcctggt acgcgctggc cgatttgagg atggcaatgc attgatgcag
     3661 atcggacaac tcggtgggct cgcgcgaagt ctggagcagg atcgggtgaa cgagcagtct
     3721 cgccgtatgg ctggcgcgct cgatgtcttt cccgagccgg taaaacgtcc atccctcatc
     3781 gtgcagcatc gtgctcgcca taatgccgtg gagggtatag caggtatctt ggacttcggt
     3841 cagaattcga tgcgggctcg cggcgatact ggcgggcgtg tagtccctcc aggccagatg
     3901 cacgcgattg atatggtgcc agacctcgct cgaaagctgg tcctggatcc ctcgcgcatt
     3961 ctccctggcg gcgctgatca aattgacgag ggcgttagga ttccccttgt cgagcgtgag
     4021 aaattccatt acgagcggcg tcgccagagg ctgcgcccgc tcctcatata gactcagctg
     4081 ccccgtgctc tccaggtacc agcgccagat tccgatctga tcctgcagat ccgcgctctc
     4141 cagaaggaga tggtagtgca cattgatgag gcgggccgtg tattcggctc gctcggtgta
     4201 ccgggcaatc cagaagcacg aggcagcggt ccggctcagc atagttgctc aatcctcgcc
     4261 atagagaacc catgtgcctt tgctgcctcc cccttgcgac gagttgacta cgagggagcc
     4321 ttcgcgaagg gccactcgcg tcaatccacc cagggacagg gtgatgtcct ggccgcagag
     4381 aacgaagggc cgcagatcga tgtggcggcc ggccagccgg aatccgccgt cgggatcgtc
     4441 gaccatacag ggaagccgcg acaaagaaac gatcggctgg gcaatgtagt tccgcggatg
     4501 cgccaggatc cgggcgcgga actcttcctg ctcagccttc gtagacgccg gtcccattaa
     4561 catgccatag cctccggact cattcactgc cttcacgacc agggacggaa ggtgctcgag
     4621 gacgtagcgc cggtcgtctt ccacactgca caggtacgtc ggcacgttgg gcaggatggg
     4681 ggattcgctc agatagaacg caatcatttt gggcacatac gcgtagatcg ctttatcgtc
     4741 ggcgacgcca ttgccgatag cgttcgccag ggcgacattc cctgcgtgat aggcccggac
     4801 gaggcccggc accccgagga cggaatccgg acgaaagact tccggatcca ggaa
//
LOCUS       DATIFQ010000080         8326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_791961, whole genome shotgun sequence.
ACCESSION   DATIFQ010000080 DATIFQ010000000
VERSION     DATIFQ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791961"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1113
                     /locus_tag="VLE22_03455"
     CDS             172..1113
                     /locus_tag="VLE22_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSB13488.1"
                     /translation="MPANLTPDYLAAEQEYKHAQTPQEKIAALERMMATIPKHKGTEK
                     LQAEIKRRLSQARKDSQKKGATHATPFYLIKKEGAGQVVLVGPPNSGKSQLVCTLTHA
                     RPEVADYPFTTRLPTPGMMPFENVQIQLVDLPPLSPEFTEPWLPQVIRYATLGVLVVD
                     LNDPGVLDEMEFIHGLLDERRLPAPKLLAGNKIDLGGARDNFAALVDLYGDRYRCVAT
                     SALTGEGLDRFARAVYDALEIVRVYTKVPGKPPELTAPYILRRGSTVEDAARHVHKDF
                     AEHLKYARLFRASGSRDGLMVDRHRLVEDEDIFEFHM"
     gene            complement(1146..1355)
                     /locus_tag="VLE22_03460"
     CDS             complement(1146..1355)
                     /locus_tag="VLE22_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13489.1"
                     /translation="MAAGKVLGRLRLWYDKQPLWLTEKLFESAGMTALGAFVIGIVCG
                     VFWLVLVIARYAQSLNDLRDLRASF"
     gene            complement(1444..4209)
                     /locus_tag="VLE22_03465"
     CDS             complement(1444..4209)
                     /locus_tag="VLE22_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13490.1"
                     /translation="MPERIYKLQPDRTVQLRGFDGLGAAAALHSATPAAFKVSGAFRD
                     PGDFAVVVLYDADNFYEHPRLKYLPDTNFDGLTLRFDVRFSGLMPLDSPKYATIDWPY
                     LDVIRPDGSTAKIRLFDHAAQVGGTYAPAQAEFLVEATGLQPYDRLTLWYLTYAFDYI
                     VPVTQPLPSAADVAAALAAQINSADWSALGALIPLRAESYGATLRIIAARPGYDGNMI
                     RMYAVAKNGRLRTVAPTAVFQGGSSDATWRVTLDFTALGIPEIRQMWLTFAPPLANGA
                     SFSDTEWQAEFTNWTVGGPEEVRALKVAGPGSVRVEEDDSWCSYTGEWGVETGFYSGG
                     YARRTAAAGNAVTVTYSCSEVHDLYVGTSLYGDRGVAGVRLNGDSETDLDCRMDVEPA
                     VVTRRKVRAAVPAGRHSVTLRLKSEGCFYLDFLEAAVAGDVQDALPPLLNASPALDYS
                     TDHTYKLPPARVLWNLDQLGYGGPMNEYIGVFWWNQRTRVNAVLPSVVVTFSGQFAAG
                     DQVFLDIGGQVCGKTVFPNEDTVVLAAHFQHFINATYVGVWATAGGNVLTITVRSPKP
                     AYSFTFQATKESPSGSSGAVTFTGSLQGGQPGQWVIDLSQTPALNRGARDWHRDLYQG
                     CKARGREIVTSASMELVNPPEGFAAKFPDGAAVETSVGFGDLVSTHCAFVSGVTEYQK
                     RVYECLAGLMAEEGLTPELQFGEFCWWYFTNRTAENPNGGMAYFHEEITAAAQAALGR
                     PLYLFSDPDDDPGVNGGADALFLRGRLRDHVAALASHLRALYSSAKLEVLYPFDVNYP
                     SPAGVHGIGGRLNRFVNLPAEWERKDTSGLDRLKVEALDFGAWSRDLGLVYQAIRFPL
                     ELGWPRDSLRHLLAVFTPGYAWEKEYLTALGEGIPVVNLWAFDHVCLFGLTPGAEWGT
                     ASARRMG"
     gene            complement(4614..5108)
                     /locus_tag="VLE22_03470"
     CDS             complement(4614..5108)
                     /locus_tag="VLE22_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSB13491.1"
                     /translation="MDKTKSIELLNHAVADELQAVHQYMYFHFHLDDQGFRPLSLLFK
                     RIAIEEMGHVERLAERILFLKGDVDMKPAGGVDRIVDPEQMLAKAALMEETGARDYNA
                     AAIACSANADSVSKQIFEQLVQDEERHFDEYDKQLDNIKRFGPSYLALQSFSTAPEPP
                     GVPA"
     gene            complement(5187..6866)
                     /locus_tag="VLE22_03475"
     CDS             complement(5187..6866)
                     /locus_tag="VLE22_03475"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB13492.1"
                     /translation="MLAAKRVLIVGGVAGGASCAARLRRLDETAEIVVLDRGPYVSFA
                     NCGLPYYVGDVIRDETKLLVATPELFRQRFNIEVRTQNEVVAIDRIAREIEVRELPSG
                     RMYRERYDALVLSPGAMPIRPPIAGIALPGIFTLRTVPDSRRIREWIEQRNARRAVVV
                     GGGFIGLEMTENLVHRGLEVTVVEMADQLMPPLDPEMADFVRRRVVTHTAHVHLGDGV
                     AGFSASEDGSLIVRTAGGSALPADLVILSIGVRPETSLARQAGLVIGDCGGIRVDERM
                     RTSDASIWAVGDAVEVRNTVTGEWEMIPLAGPANRQGRIAADVICGRNTRFRGVQATT
                     VCGFFGLTVALTGATEKSLRRAGITDYEAIYVHPGHHAGYYPGAKPIHLKLIFRKCGG
                     EILGAQAVGDEGVERRIDAISMAIQARQTVFDLEEAELCYAPQYGAAKDPVNVAGMVA
                     ANVVRGDVELAHWGDVPGADALLLDVRDATEFAGGSIEGSVNIPLGQLRDRARELPRD
                     REIWVSCGVGQRAYYACRLLAQLGFRVRNLPGGYHSYRSWYPHGLHRIPPR"
     gene            7188..>8326
                     /locus_tag="VLE22_03480"
     CDS             7188..>8326
                     /locus_tag="VLE22_03480"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB13493.1"
                     /translation="MTVLLTLLSSAVFGQEYRGRIQGTVMDTTTAAIPGAKVTLLNNR
                     TGVEVVRQSNEMGRFLFDLVEPGSYNLTVENAGFSKFVLENINVPSRADLTLDATLKP
                     GEVRETISVVAEAATLQLNTAKLETTVDQKLTQSLPQLYRNPFLLAQLDPSVESTSWG
                     EDNPYDTWASNNLRIGGSGQFTNDLQVDGSPSGISVKTGYVPSPDMVQEVNILQNAVD
                     AEYGHSSGSAISVVMKAGTNEYHGSAFFQGARPYWNALENRVYRSENQTRNNMYGATL
                     GGPIIKNKLFNFAGYEGWNKTEPAMLYNTVPTDLERTGNFSQTLTDEGGLRPIYDPWT
                     TQTASDGTITRTPFTDNTIPSTRIAPVASKYTAALWKANGPGIDA"
BASE COUNT         1640 a         2634 c         2522 g         1530 t
ORIGIN      
        1 ctggcccggt tcggttttgt gctgctgatc gtcgcgatct cggtgtcgtc gttggggcgg
       61 tacctctccc tgtggagctt ccagggtacc caagtgatcc tccggatgtt ctggtaggac
      121 gggcggaccg ccggactcgt cggcggtgtt attcttgctg ccgagagcgc tatgccggcg
      181 aacctgacgc ccgactatct ggcggccgag caggaataca agcacgcgca gacgccgcaa
      241 gagaagatcg cggcgctcga gcggatgatg gcgaccatcc ccaagcacaa ggggacggaa
      301 aagctccagg ccgagatcaa gcgccgcctg tcgcaggcac ggaaggattc tcagaaaaag
      361 ggcgccactc acgcaacgcc gttctacctc atcaagaaag agggcgcggg acaggtggtg
      421 ctggtcggcc cgcccaattc cgggaagtcg cagcttgtgt gcacgctcac gcatgcgcgc
      481 ccggaggtcg cggactatcc cttcaccacg cgcctgccca cgcccggcat gatgccgttc
      541 gagaacgtgc agatccagct tgtggacctg cctcctctgt caccggaatt caccgagccg
      601 tggctgccgc aggtgatccg ctacgcgact ctaggagtgc tcgtggtgga tctgaacgat
      661 ccgggcgtgc tcgacgaaat ggagttcatt cacggactgc tggacgagcg gcgccttccg
      721 gctccgaagc tgcttgccgg gaataagatt gacctgggcg gcgcccgcga caacttcgcg
      781 gcattggtgg acctttatgg ggaccgttac cgctgcgtcg ccacctccgc gttgacgggc
      841 gagggactcg accgctttgc acgggcggtt tatgacgccc tcgagattgt tcgcgtgtat
      901 acgaaggttc cggggaagcc gccggagttg acggcgccat atatcctccg gcgtggctcg
      961 acggtcgagg acgccgcccg tcatgtccat aaggacttcg ccgagcacct gaagtatgcc
     1021 cgactgttcc gtgcctcagg cagcagggat ggcctcatgg ttgaccgcca ccgcctggta
     1081 gaggacgaag acatcttcga gtttcatatg tgaggagcgg ctgcttgggg atttgacggt
     1141 gccgttcaga aactcgcacg caggtcgcgc agatcgttga gggattgggc gtatcgggcg
     1201 atcacaagaa caagccagaa cacgccgcag acgatgccga tcacaaaagc gccgagagcc
     1261 gtcattcccg cactttcgaa gagcttttcc gtcagccaga gcggctgctt gtcgtaccag
     1321 agacgaagcc ttcccaggac tttccccgcg gccatctttc cccttccctg gcgaacagtg
     1381 tagccggaca cgacagggaa agcaacaaga aatcgtgtgt cgagttttcc aacgctccgg
     1441 tcgtcagccc atccgccttg ccgaagcagt gccccattcc gcccccggag taaggccgaa
     1501 gaggcagacg tgatcgaacg cccacaggtt cactacggga attccctccc ccagtgccgt
     1561 caggtactct ttctcccacg cgtaccccgg cgtgaacacg gccagcaagt ggcgcagcga
     1621 gtctcggggc cagccaagct ccagagggaa gcggatggct tgatagacga ggccgagatc
     1681 ccgcgaccac gcgccgaagt cgagcgcctc cactttgagc cggtccagtc cgctcgtgtc
     1741 cttacgctcc cattcggccg gcagattgac gaaccggttc agccggcctc cgatcccgtg
     1801 cactcccgcg ggtgacggat agttgacgtc gaatggatac agcacctcga gcttcgccga
     1861 cgaatagagc gcccgcaagt gggaggccag cgccgccaca tggtcgcgca gccgaccgcg
     1921 gaggaagagg gcgtcggcgc cgccgttcac gcccgggtcg tcgtccgggt cggagaacag
     1981 gtacagcggc cttcccaatg ccgcctgcgc cgccgcggtg atctcttcat ggaagtatgc
     2041 catgcctccg ttcgggtttt ccgcagtccg attggtgaaa taccaccagc agaattcgcc
     2101 gaactgaagc tccggagtaa gtccttcctc cgccatcagg cccgccaggc actcgtagac
     2161 ccgcttctgg tattcggtga cgccggagac gaatgcacag tgtgtcgaaa cgaggtcgcc
     2221 gaaccccacc gaggtctcga cggcggcccc gtccgggaac ttcgcggcga aaccttccgg
     2281 cggattcacc agctccatgg acgcggaagt tacgatttcg cgtccccgcg ctttgcatcc
     2341 ctggtagagg tcccggtgcc agtcccgcgc accccgattg agagcgggcg tttgcgaaag
     2401 atcgatcacc cactgccccg gctgtccacc ctggagcgag ccggtgaacg tgacagcacc
     2461 actcgatccg gaagggctct ctttcgtcgc ctggaatgtg aacgagtagg ccggtttggg
     2521 cgagcggacg gttatcgtca gtacattgcc cccggccgtt gcccagacgc caacgtaggt
     2581 tgcgttgatg aagtgctgga aatgcgccgc cagcaccaca gtgtcctcat tcgggaacac
     2641 agtcttgccg catacctgcc cgccgatgtc gaggaacact tgatcgcccg ccgcgaactg
     2701 gccgctgaac gtgacgacca cactcggaag cacggcattc acgcgcgttc gctgattcca
     2761 ccagaacacg ccgatgtact cgttcatcgg cccgccgtag cccaattgat cgagattcca
     2821 gagtacgcgg gccggcggca gcttgtatgt atggtccgtg ctgtagtcga gcgcgggcga
     2881 cgcgtttaac agcggcggta gcgcgtcctg gacatcgccc gccaccgccg cttccagaaa
     2941 gtcgaggtag aagcagccct cggacttgag ccgtaacgtc accgagtgcc ggccagccgg
     3001 caccgccgcc cgcacttttc tgcgcgtcac cacggccggc tccacgtcca tccggcagtc
     3061 aaggtccgtt tccgaatcgc cgttgagccg cactccggcc acgccacgat ccccgtaaag
     3121 agacgtgcct acgtacaagt catgcacctc gctgcaagaa tacgtcaccg tcaccgcatt
     3181 gccggcggcc gcagtgcgcc gcgcatagcc gcccgagtag aaccctgtct cgacacccca
     3241 ctctcctgtg tagctgcacc aggagtcatc ctcctctacg cgcacgctgc ccggcccggc
     3301 caccttcagc gcccgcacct cctccggtcc gccgaccgtc cagttcgtga actcggcttg
     3361 ccattccgtg tcggagaagg aagcgccgtt cgcgagcggc ggtgcgaagg tgagccacat
     3421 ctgccggatc tcggggatcc ccagagccgt gaagtccagg gtcacccgcc aagtggcgtc
     3481 cgaacttccc ccctggaaca ccgccgtggg cgccacggtc cggagccggc cgttcttcgc
     3541 aacggcgtac atgcggatca tgttgccgtc gtaccccggg cgcgcggcga tgatccgaag
     3601 cgttgctccg taagactccg cccgcagcgg gatcagcgct ccgagtgcgc tccagtcggc
     3661 cgagttaatc tgcgctgcta gcgctgccgc cacgtctgcg gcggagggca aaggctgcgt
     3721 gaccggaacg atgtagtcga aggcgtaggt taagtaccac agcgtcaggc gatcgtacgg
     3781 ctgaagcccg gttgcctcga ccagaaactc ggcttgggcc ggcgcgtagg tccccccgac
     3841 ctgcgctgcg tggtcgaaca ggcggatttt ggccgtggac ccgtcagggc ggatgacgtc
     3901 gaggtatggc cagtcgatgg tggcgtattt cggcgaatcg agcggcatca gcccggagaa
     3961 tcgcacgtcg aaccgaagcg ttagcccgtc gaagttcgtg tccggaagat acttcagacg
     4021 tggatgctca tagaagttgt cggcgtcgta gaggaccacg acggcgaagt cgcccgggtc
     4081 tcggaaggca ccgctcactt tgaaggcggc gggcgtcgcg gaatgcagtg ccgccgcggc
     4141 gccgagaccg tcgaaaccgc gcagttggac ggtacggtca ggctgcagtt tgtagattcg
     4201 ctccggcaag tgagtgcctc ccgttttgat cgtacgggtg gcgctcaccc ggaacctcac
     4261 caatgagttg caaaaacaac cgcatctcat tgacgcagca taactaaacg aaatataccg
     4321 ccgttgtgac tggacggcgg gctgtccgtg atgcgggatg atgtacgtga cgtgcgcggt
     4381 cggctataat tgcggagatc gcggtacacc gctcatgagc aaagtcggca aaacggtgct
     4441 ctggttgcta gccgctttgg cggcggtgtg gattgccacg cactggcagg aattcctgag
     4501 ccaattgcgc agcatcggaa tgatgccgtt cattgaggag cgcctgcact ccagcggagt
     4561 caccccacgt tcgccgctgg cgaaactacc ggctggaacc gccttacccg cggttatgcc
     4621 ggcacaccgg gaggctcggg agccgtgctg aacgactgaa gagccaggta gcttgggccg
     4681 aaccgcttga tgttgtccag ttgcttgtcg tactcgtcga agtgccgctc ttcgtcctga
     4741 acaagctgct caaaaatctg tttcgagaca gaatcggcgt tcgccgagca ggcgatcgcg
     4801 gcggcattgt agtcgcgagc gccagtctcc tccatcaacg ccgctttcgc cagcatttgc
     4861 tcgggatcga cgatccggtc gacaccgccg gccggcttca tgtccacgtc gcccttcaga
     4921 aacagaatcc gctcggcgag gcgctccaca tgtcccattt cctcaatcgc gatgcgcttg
     4981 aacaggagtg agagtgggcg gaagccctgg tcgtcgagat gaaagtgaaa gtacatgtac
     5041 tggtggactg cttgtagttc gtcggcaaca gcgtggttca ggagttcgat gcttttggtc
     5101 ttatccataa gattgcctcg ccttcatcaa ctatcatagg gccaacggag cagctcaggc
     5161 gccggcctgt gcttccgaca gcaccgtcat cgaggcggga ttcggtgcaa cccgtgcggg
     5221 taccacgaac ggtacgagtg ataccctccc ggcagatttc tcacacgaaa cccgagttgc
     5281 gcaagcaggc ggcaggcgta gtacgctcgc tgcccgactc cgcaactcac ccagatttcg
     5341 cggtcccggg gcaattcacg ggcgcggtct cggagctggc cgagtgggat attcaccgaa
     5401 ccctcgatgg agccacccgc aaattcggtg gcgtctcgca cgtcgagtag caaggcgtcg
     5461 gcgccgggca catctcccca gtgggcaagt tccacatcac ctcggacgac gtttgccgcg
     5521 accattccag cgacgttcac ggggtccttg gcagcgccgt attgcggcgc gtaacagagc
     5581 tcggcctctt cgagatcgaa gaccgtctga cgggcctgga tggccatcga gatcgcgtcg
     5641 atgcgccgct ctactccctc atcgccgacg gcctgggctc cgagaatttc gccaccgcac
     5701 ttacgaaaga tcaacttcag atgaatcggc ttggcgccgg gatagtaacc cgcatggtgc
     5761 cccggatgca cgtagatcgc ttcgtagtcg gtgatgcccg cgcggcgcag ggacttttcg
     5821 gtggccccgg ttagggcgac ggtcaagccg aagaacccgc acacggtcgt ggcctgtact
     5881 ccgcggaacc gtgtgttgcg cccgcagatg acgtccgccg cgatgcggcc ctggcggttg
     5941 gccggcccgg ccagcggaat catctcccac tcacccgtca ccgtgttgcg tacctcgacc
     6001 gcgtccccga ccgcccaaat ggaagcgtca ctcgtccgca tccgctcgtc cacccggatg
     6061 ccaccgcaat cgccgattac cagccccgcc tgccgggcca gactcgtctc gggccgtacg
     6121 ccgatcgaaa gaatcacgag gtccgccgga agcgcagatc caccagccgt tcggacaatg
     6181 aggctgccgt cttccgacgc gctgaaaccc gcgacgccgt caccgagatg gacatgagcc
     6241 gtgtgcgtaa ccacgcgtcg gcggacgaaa tcggccatct cggggtcgag cgggggcatc
     6301 aactggtccg ccatctctac gaccgtgact tcgaggccgc ggtgtacgag gttttcggtc
     6361 atttccaggc cgatgaatcc accccccaca accaccgccc ggcgggcgtt cctctgctcg
     6421 atccactccc ggatgcggcg gctgtccggc acagtacgca acgtgaatat gccgggaagg
     6481 gcgattcccg cgataggcgg ccggatgggc atcgcgccgg gcgagagcac gagggcatcg
     6541 taccgctctc gatacatcct cccggaggga agctcgcgaa cctcgatctc gcgcgcgatc
     6601 cggtcgatcg cgactacctc gttctgagtg cgtacctcga tgttgaagcg ttggcgaaac
     6661 agttcgggcg tcgcgaccag cagtttggtc tcgtcacgga tgacgtcacc cacgtagtac
     6721 ggaaggccgc agttggcgaa ggacacgtat ggcccgcgat cgagcactac aatttcagcg
     6781 gtttcgtcca ggcgccgaag gcgcgcggcg caggatgccc cgcccgccac accacccaca
     6841 atcaagaccc gttttgcagc caacatgtca gttccagttt cgcccatgcc aatttgcggt
     6901 cgtcagattt tgcgttacga aactctaata ttcgcacgag cttggtcaag tcttgctgcc
     6961 ctcccgtcac tgcctggcat tcgcccttga aagcgcgcaa ctgtacggca agaaaaaaga
     7021 gcatgcgtta attaaatttt gttaattaat gaccgagagc catccattca gtaccgaact
     7081 cgatatataa tggcgcgcga cgaggtttgt cgagccgcaa tcgcggactg caaaaagggg
     7141 gatatgtgta ttacttgagg cgaaggactc ttttctcgat agcgtgcatg actgttttgc
     7201 tgacgctgct atcgagcgca gtgtttgggc aggagtatcg cggcaggatc cagggcactg
     7261 tgatggacac cactaccgcc gccattcccg gcgccaaggt aaccctgctg aacaacagaa
     7321 ccggtgtgga agtcgtccgg caaagcaacg agatgggccg cttcctgttc gacctggtgg
     7381 aaccggggag ctataacctc accgtcgaaa acgccggttt cagcaaattc gtgctggaga
     7441 acatcaacgt gccatcccgc gccgacctga cgctggatgc cacgttaaaa cctggagagg
     7501 taagggagac aatctcggtc gtcgcggagg cggcgacact gcaactgaac accgcaaaac
     7561 tcgagacgac ggtcgaccag aaactgaccc agagccttcc gcagttgtat cgcaatccct
     7621 tcctattggc gcagttggac ccgtcggtcg agagtacgtc ctggggagag gacaaccctt
     7681 acgacacttg ggcatccaac aacctaagga tcggaggcag tggacagttc accaatgatc
     7741 tgcaagtgga cggcagtccc tccgggatca gcgtgaagac gggctacgtt ccctctccgg
     7801 acatggtcca ggaagtcaac atccttcaga atgcagtgga tgccgagtac ggacacagct
     7861 ccggctccgc aatcagcgtg gtgatgaagg cgggcaccaa cgagtatcat ggaagcgcgt
     7921 ttttccaggg cgcacgtcca tactggaatg cgcttgaaaa ccgcgtgtac cggagcgaga
     7981 accagacccg caacaacatg tacggcgcca ctctcggcgg gcccatcatc aagaacaagc
     8041 tgttcaactt tgcgggctat gaaggatgga acaaaactga acccgccatg ctgtataaca
     8101 cggttcccac cgatctggag cggaccggga atttttcgca gacgctgacg gatgaaggcg
     8161 gtttgcggcc gatttacgat ccctggacca cgcagacggc gtccgatggc acgattaccc
     8221 gcacgccctt cactgacaac acgattccgt ccacccggat tgctccggtc gcttcgaagt
     8281 acacggctgc gctctggaaa gcgaacggac cggggattga cgcctt
//
LOCUS       DATIFQ010000081        17066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_217793, whole genome shotgun sequence.
ACCESSION   DATIFQ010000081 DATIFQ010000000
VERSION     DATIFQ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17066
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217793"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VLE22_03485"
     CDS             <1..966
                     /locus_tag="VLE22_03485"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSB13494.1"
                     /translation="RGDLVNQPGFTDGERTPDPRLMLRGYERAALTLNFIRALVTRGF
                     VDPHHLEYWDLDFVKDSPLADEYRRMARGIAESLRFMENILGAHPGEMHWVDFFTSHE
                     GLLLPYEQAQTRRVPRREGWYNLSTHFPWIGMRTADPVGAHVEYFRGIVNPVGVKVGP
                     DTSPETLRRLISILHPDNEPGRLTLIHRFGNDRITACLPPLIEAVRSTGKTVLWCCDP
                     MHGNTRLTAGGIKTRRFEDILGEVDKAFDIHSACGSDLGGIHIELTGEDVTECIGGAR
                     GLSEVDLARAYESHLDPRLNYEQSLELAMFVAQKMRERKSALYLR"
     gene            complement(946..2139)
                     /locus_tag="VLE22_03490"
     CDS             complement(946..2139)
                     /locus_tag="VLE22_03490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB13495.1"
                     /translation="MNHVASANRLIFLTCLGILIFGMVSATLGTLQPYWAGIGLNPEQ
                     TAHVALAQAVGMILASLIAGPLCDNKGKKTGLMLGLGSLLIGLAALPHVVSFVMIIAA
                     LFLVGLGGGILVTSVNALLSDISAERRAAVLNFVSVFFGLGGMLAPFLVANLLSRNSA
                     ESAVQFFYVMVALVAVTLVVYAATKMPPPTGQRGFKFSEAGQVLGRPALWLLVVYMFL
                     YISCELNYWNWLTRHLMAQGIKDSVAQNILSFGFALGLLLGRIAVLPILMRVSVVTVT
                     LGATALMAVSTFCVLQTNDPALVGLAVFFAGMAMAPVFPNIVAMTGGAFDYATGTAIG
                     IVITSGWAGLAVSSRIIGAIAGDDPARLGTALYLLPAFSLAMILVNLALRPFLRRPRS
                     AEVKG"
     gene            2287..2874
                     /locus_tag="VLE22_03495"
     CDS             2287..2874
                     /locus_tag="VLE22_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13496.1"
                     /translation="MRALAVGTWMIVGLAAIAIDPSFGQLPEDLKRKFDEAERRIVRL
                     PPTAFHELPANVIRELQRRGCTVPQEAYTKKPHNVIKGEFAKPGQIDWAVLCSVRGVS
                     SILVFWNGSEKNPSAIAPAEDRNYLQGITAEQFGFSRGITSVGKGLIMKHYRAYGGPT
                     PPPTGHQGIDDAFIEKGSRTWYFHGGEWLQLTGAD"
     gene            3073..6162
                     /locus_tag="VLE22_03500"
     CDS             3073..6162
                     /locus_tag="VLE22_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB13497.1"
                     /translation="MLVLLALAASGQQRLSGIAGTVYDTRGAVVSGASITVTGVDTGV
                     RREAVSGPDGVYRVPSLELGRYAVTASKAGFKSSRHEDVLLELEREAVVDHRLEVGDF
                     SQTIVVVGQSRLIDASAATLSGLVRSSAIEHLPLNGRDYTRLALLQPGTAVAQSQSRD
                     VNNGYGVPISFAGSRPFQNGFLLDGISLNSFHNSAPASVNGATLGVEAIQEFSVIAGP
                     FSAEYGRSGGGIMNAVTRSGGNEYHGSAYYFHRNDNLDARNFFDTAKPEFRRHQGGIS
                     VGGPLRRNRVFFFSNYEAFRVARGNTTVNTTLSSEARRGELLSGTMTVDSAVEKAAAL
                     YPLPNGTIFGDTGLFVFTNQLRARQDMGTGRIDWNPSDTDRVFARYSVDDGLRSQHTD
                     FALGRTTNISRAQSLSLEHTHLFGPSWLNTARAGFARDRTVRGASRAAVSAVDDPSLA
                     FLPSGGSVGIIEVTGLTTLPGGSGALDADLHAFTSYQLSDDALRTGGKHMFRFGGRLE
                     RTWLNSDSQNSVSGIYQFRSIAEFLTNRPNRFQAQLPGSDTVRGFRQWTGALYVQDHW
                     RIHERVTAELGLRWEVASVPAEVQGKLSNLDQLTDARMREGQPFFRQPSAVFLAPRAG
                     LAWDTRGAGRTVLRAGYGIYPDLILTQFLLLAGMRNPPYFFRGSLRALQKGDFPSNGY
                     RRLVESGTAELRIDRFDPAPNQPYVQHWTLQLEQRLSRLATARAAYLGSHGLNLSSVT
                     DDANLVTPVRLADGRLYYPSDGKKLNPNFSAIRNRLFDAHSFYHGLLTSLERPLDHGL
                     QVQASYTFSKSIDDSSNFFSPTESTNYSSSAVIDNPRFNRGLSAHDVPHRLAVSGIWE
                     APLPKRTNWLQPLRGLVLSAMYTYSSGIPFSAWLGYDGAGTKAERSDWRSGQRPDIIE
                     GSGRRLFTGNPDGWVDLSAFRRPERGFLGNLGRNTIRGPNLSNLDFSTNWRRPLKERA
                     TVELRFEVFNSLNRTNLSIPSAERMQIFTVDGIREDAGRITEALDSREIQIGLKISF"
     gene            6162..7778
                     /locus_tag="VLE22_03505"
     CDS             6162..7778
                     /locus_tag="VLE22_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13498.1"
                     /translation="MLDSLQMLLRFSTGLRKHLSSPLTIQECRDIVVKSIQEREVSFL
                     DLLRRAIYENPRSPYLALLSRAGAEYGDVSAIVRQDGVDAALEKLYDGGVRIRLEEFK
                     GLRPIERSGLTLAVKAEDFDNPLLRRDFEVATSGSTGRRRRLAIDVDLLLQEAAYTRV
                     IEEAHGVFERPKAIWRPVPPGSSGLKAVLRAEKCGRSLDKWFSQTHISWGPRGWTSAV
                     FTTSAVMIGRFCGRSIPMPEHVPLDEARRVAEWMSEQASAGRPAYLSAPSSTVVRICQ
                     AARVGGLDITGSVFRVSGEPYTEGKRRVVDSCGVRTFSGWSLAECGGVAGGCARSEAL
                     DEMHLLTGMVALIQRPVSLRGEAGSVDAFHLTTLRRETPKVMLNVDAGDYGMVSRRRC
                     GCPLEELGMHVHVHGIRNYEKLTSNGMHIIGSDILELVEDFLPRRHGGAPTDYQFVEE
                     SGGAESRIAIVVSRRVPNLDEGALLTHVTAFLTGKSRAHRMMINVWEQGGSLRVVRAE
                     PHTTSTGKTPALRVIQELETETGEAKRVGS"
     gene            7768..9072
                     /locus_tag="VLE22_03510"
     CDS             7768..9072
                     /locus_tag="VLE22_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HSB13499.1"
                     /translation="MALDAAVQRRSRWSWVRSLSGPGLVFVLAALGPQDLVSNAVAGA
                     DHGYSLLWTLILLAIARYTVLEASGRYVVVSGESLVEGYARCGRWIAWLLLVSILIKR
                     HLTNLSLILLMGVAVNLLFPLDHPRGAAFWSLALWMLGLWVMLWGRYARIEKWFRGLA
                     ILLACCLILVAALARPDPLLILKGLTIPSMPQGSSLPGTLFVLMALMGAGAGSVNNLK
                     YPAFLLETGWDSRHYLRANRRQALFSSGGLLLAAVLVQVAASASGQQAEGIRTAGDLT
                     AIFGRALGEPGRLAITVGLAAAVFSTFVGANTGYSLIVSDLVHNVLRRNQSRTGTRPP
                     GSLPAYRWALLVFTVPSLYVLVTNWQPVWMVLLSAAATVALLPLTVPVLLILTSDRER
                     MGEQASGWATKIIMLLVVLAALYLTWQNARTLLAGGFSSAPG"
     gene            complement(9069..9569)
                     /locus_tag="VLE22_03515"
     CDS             complement(9069..9569)
                     /locus_tag="VLE22_03515"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB13500.1"
                     /translation="MRYGLVGLLLSLWCLLRRVGRQGKAPTAKSPKSLRERAERGDAV
                     AQFLLGARYDLGQGMAQDVAEAAHWYRKAAVQGYAPAQVALGLMYALGGGIAQDYVEA
                     HLWINLAASHASGSRQKRYTDLREAFAGVMTPAQIAEAQRRAGEWKPTTGGHPEPINS
                     RQLRVA"
     gene            9780..10901
                     /gene="tal"
                     /locus_tag="VLE22_03520"
     CDS             9780..10901
                     /gene="tal"
                     /locus_tag="VLE22_03520"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HSB13501.1"
                     /translation="MNSNPLRRLKTLGQSIWLDYIRRDLITSGELRRLIEEDGVRGMT
                     SNPAIFEKAITGSRDYDDSIRALALDGKGVQAIYDVLTMRDVQSAADEFRPLYDRTGG
                     KDGYVSLEVNPHLARDTKGTIQEARRLWDSLNRPNVFIKVPATAEGLPAIQQLIGEGI
                     SVNVTLLFGLPRYRKVAEAYIAGIEARAAQGKALNHVASVASFFVSRIDGLVDPMLEK
                     LATQSGEKAELAKNLHGQVAIANAKMAYQIYKEIFGHDRFKRLAGSGAGVQRLLWAST
                     GTKNPDYSDVKYVEALIGLDTINTVTIETLNAYRDHGDPKARLEQDVMEARWVLEQLP
                     ELGINIDNVTQQLEDEGVQKFNQPFDKLMETLAQKSSQT"
     gene            11012..11296
                     /locus_tag="VLE22_03525"
     CDS             11012..11296
                     /locus_tag="VLE22_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13502.1"
                     /translation="MPGQRVLFGTSGHRGSAFDKAFNEWHIPAISQAICLYRKQKEID
                     GPLFLGTDTTLELIYRFKQRLCYLLLKKHRTRKQCQALVPRFLRAVYQLR"
     gene            11679..13022
                     /locus_tag="VLE22_03530"
     CDS             11679..13022
                     /locus_tag="VLE22_03530"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HSB13503.1"
                     /translation="MSDLPNRPGPEFAAWIGIDWGDKKHAWAVQAEGSTQVEQGEMAH
                     TPEAIQEWAMELRRRFPTGRLAVALEQSRGPLVFVLSKYEHLVLYPIHPTSAANYRKI
                     FRPSGAKSDGPDAAIHLDMLVKHRDQLRPLHPDTVETRTLQLLVEDRRQLVNERTRIS
                     NRLVADLKSYYPQPLQWFEKPYAPVTLDFLARWPTLEELQKARPATLQEFFKQHQCRD
                     DAKNQARIEDMHKTIAATHDGAVRIAGRAAVCAAVRLLRGLSQSIQDYDQKIEELAQA
                     HPDFEIFVSLPAAGAAFAPRLIAAFGAQRDRYRDAAEVQQFSGIAPVTEASGNQRWVH
                     WRWSCPKFLRQTFHEWAAQTIQQCDWAAAYYQVQREDKKKSHHAAVRSLAYKWIRIVF
                     RCWKDRKPYDESRYEGALKRQHQKAAAKSVQPSPFELPVQWISCAGFQKLASKNG"
     gene            13051..13203
                     /locus_tag="VLE22_03535"
                     /pseudo
     CDS             13051..13203
                     /locus_tag="VLE22_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13264..13357)
                     /locus_tag="VLE22_03540"
     tRNA            complement(13264..13357)
                     /locus_tag="VLE22_03540"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13321..13323),aa:Ser,seq:gct)
     gene            13479..13919
                     /locus_tag="VLE22_03545"
     CDS             13479..13919
                     /locus_tag="VLE22_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HSB13504.1"
                     /translation="MLRLTKKADYGLIAVTHLAAAGERGAVSAKEIADAYHIPLPLLA
                     KILQKLARRGVLASVHGTNGGYRLSRDASRISALEVIRAIDGPVALTSCGGSHGDCGQ
                     SEKCTVREPLRVVHDGIMELLAGISISDLSQDRRRVPLTMIQTS"
     gene            13930..15144
                     /locus_tag="VLE22_03550"
     CDS             13930..15144
                     /locus_tag="VLE22_03550"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HSB13505.1"
                     /translation="MTKPIYMDNHATTPIDPRVLEAMMPYFTGKFGNAASRNHSYGWE
                     AEQAVEKARQQIARLIGASPKEIVFTSGATESDNLAIKGVAGMYAEKGNHIITVPTEH
                     KAVLDTCKRLQKHGYEITFLSPGRYGIIDLDELRGAITDKTILITVMHANNEIGVLQP
                     IREIGKIARERGVLFHTDATQSVGKVPVNVNDDNVDLMSISAHKMYGPKGVGALYVRR
                     KNPRVQLTCQMDGGGHERGMRSGTLNVPGIAGLGEACAICQQEMAKEAIRLSDLRDKL
                     KAKLEGGLDEVFVNGSMEHRLPNNLNMSFAYVEGESLLMGINDVAVSSGSACTSATLE
                     PSYVLKALGVGDDLAHTSIRFGLGRFNTEEEVDYVAARVIEVVKRLRELSPLYEMAKE
                     GVDLKTVDWVAE"
     gene            15193..15606
                     /gene="iscU"
                     /locus_tag="VLE22_03555"
     CDS             15193..15606
                     /gene="iscU"
                     /locus_tag="VLE22_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HSB13506.1"
                     /translation="MAYSDKVLDHYNNPRNVGTMDKKDPSVGTGMVGAPECGDVMKLQ
                     LKVNPETGVIEDAKFKTFGCGSAIASSSLATEWVKGRTVDQALEIRNTDIVNELSLPP
                     VKIHCSVLAEDAIKAAIDDYKRKQEALRAAEPAIS"
     gene            15634..15960
                     /locus_tag="VLE22_03560"
     CDS             15634..15960
                     /locus_tag="VLE22_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HSB13507.1"
                     /translation="MIQVTPKALDKIRQAFTRHGVSEAGGLRLGVQGGGCSGMSYLFR
                     FDPKERPNDNVYNFEGVRIFIDPKSLVYLKGMTLDYKESLIHSGFVFENPNAKKSCGC
                     GTSFSA"
     gene            15960..16580
                     /gene="hscB"
                     /locus_tag="VLE22_03565"
     CDS             15960..16580
                     /gene="hscB"
                     /locus_tag="VLE22_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HSB13508.1"
                     /translation="MHDAESCWKCGEPSAQSLFCRFCNTLQAPSSDYYRFLGLERRLS
                     LDPDDLQRRFYSLSRLVHPDRYLRSAANEQRFALEATALLNDAYRTLRDPVARAEYFL
                     KEEGFDSGGQKSNNVPPEMLEEVFDLNEALEELRGGDPSVRPQLEAARRRFRSLREDV
                     DGDLEARFREYDRGRQREILGKIRALLNRRRYIENLLHDVERELMN"
     gene            16584..>17066
                     /locus_tag="VLE22_03570"
     CDS             16584..>17066
                     /locus_tag="VLE22_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSB13509.1"
                     /translation="MSDTFQIDFSASDRPLVVGIDLGTTNSLVAFMDLTGPRVIPGDD
                     GNNLVPSVVSYSRAGEIVAGRLAREMLITEPERTVYSVKRLMGRGVADVQEELKLFPF
                     RIAEGSESVIRLELGERKFTPPEISAFILRQLRQNAEADLGETVTKAVITVPAYFNDA
                     Q"
BASE COUNT         3498 a         5131 c         5276 g         3161 t
ORIGIN      
        1 cggggcgacc tcgtcaacca acccggattc accgacggcg aacgaacgcc ggatccgcgc
       61 ctgatgctgc gaggatacga gcgcgccgca ctgacgctga atttcatccg ggcgctggtg
      121 acgcgcggct tcgtggatcc acaccacctc gaatactggg acctggactt tgtcaaagac
      181 tccccgctag ccgacgaata ccgccggatg gcgaggggca ttgccgagtc actgcgcttc
      241 atggagaaca tcctgggcgc acacccgggc gagatgcact gggtcgattt cttcaccagc
      301 cacgagggac ttctgcttcc ctacgaacaa gcgcaaacgc gccgcgtacc gcggcgggaa
      361 ggctggtaca acctctcgac gcacttcccg tggatcggca tgcgaacggc ggacccggtc
      421 ggcgcgcacg tcgagtattt tcgcggcatc gtcaacccgg tcggcgtcaa ggtcggaccg
      481 gacacgtcgc cggagacact gcgcaggctc atctcgatcc ttcaccccga caacgaaccg
      541 ggacggctga ccctgattca ccggttcggc aacgaccgga tcaccgcatg ccttccaccg
      601 ctgatcgagg ccgtgcggtc gacggggaag acggtgctct ggtgctgtga cccgatgcac
      661 ggcaacaccc gcctgaccgc cggcggcatc aagacgcgcc gttttgagga catcctcggt
      721 gaagtggaca aggcgttcga catacattcc gcgtgcggca gcgacctcgg aggcatccac
      781 atcgaactga cgggcgagga cgtcaccgag tgcatcggcg gcgcacgcgg cttgagcgaa
      841 gtggacctgg cgagagcgta cgaatcccat ctcgaccccc gcctgaacta cgagcagtca
      901 ctcgaactgg cgatgttcgt tgcgcagaag atgcgggaga gaaaatcagc cctttacctc
      961 cgctgaccgg ggtctccgca ggaacgggcg aagcgccagg ttcaccagga tcatcgccag
     1021 cgagaatgcc ggcagtagat acagggcggt gcccaggcgc gccggatcgt cgccggcgat
     1081 cgcaccgatg atgcgcgaac tgacggccaa cccggcccat cccgaggtaa tcacgatccc
     1141 gatcgccgta ccggtggcgt agtcgaaggc tccacctgtc atggccacga tgttggggaa
     1201 cacgggagcc attgccatac ctgcaaaaaa gaccgccaaa ccgacgagcg ccggatcgtt
     1261 ggtctgcaac acacagaacg tgctcaccgc catcagcgct gtggccccga gtgttactgt
     1321 caccacactc accctcatca ggatgggcag aacggcgatt ctgccaagca gcaggcccag
     1381 agcaaaaccg aaagagagga tattctgcgc cacgctatcc ttgatgcctt gcgccatcag
     1441 gtgcctcgtc aaccagttcc agtaattcag ctcgcaggaa atatacagga acatatacac
     1501 gacgagcagc cacaacgcgg gacgcccgag cacctgtcca gcctcggaga acttgaagcc
     1561 gcgctgaccg gtcggaggcg gcattttcgt ggccgcatac acgaccagag tgacggcgac
     1621 gagcgccacc atcacataga agaactgcac ggcggattcg gcggaattcc tggacaacag
     1681 gttggccacc aggaaaggcg ccagcatgcc gcccagtcca aagaaaacgc tcacaaagtt
     1741 cagaactgcc gcccggcgct cagcgctgat atcgctcagc agtgcgttta ccgaagtgac
     1801 caatatgccg ccgccaaggc cgacgagaaa cagagcagcg atgatcatta cgaagctaac
     1861 cacgtggggc aacgcggcca agccgatcaa cagcgaaccc agcccaagca tcagaccggt
     1921 cttcttgcct ttgttgtcac acagcggacc cgcgatcaga gaggcgagaa tcattccgac
     1981 cgcctgtgcg agcgccacgt gcgcggtctg ttccggattc aatccgatgc cggcccagta
     2041 aggctgaagc gtgcccaacg tggcggagac cattccaaaa atcagaattc ccaagcaggt
     2101 aagaaaaatc aggcgattgg ccgaggcaac atgattcatg agattctcac ttccagctcc
     2161 cagaagccga tgtggtgcgc cgggacgatt acgaaggacc attctaaccg caatttccgt
     2221 ctataacggc gtcggcatag tctaagttgc gaaaatgggc gagtgcagct tacgaggaga
     2281 agaggaatgc gtgctttagc ggtaggaacg tggatgattg tggggcttgc ggcaatcgcg
     2341 atcgacccgt ccttcggtca gttgccggaa gacctgaagc ggaagttcga tgaagccgag
     2401 cggcgaatcg tgcgactccc gccgacagcg ttccacgagc ttccggccaa cgttatccgg
     2461 gaactgcaac gccgtggttg caccgtcccg caagaggcgt acacgaagaa gccgcacaat
     2521 gtcatcaagg gtgaatttgc caaacccggc caaatcgatt gggctgtctt gtgctcggtc
     2581 aggggcgtct ccagcatcct cgttttctgg aacggctcgg agaagaaccc cagtgcaatc
     2641 gccccggcgg aagaccgcaa ttatcttcag gggatcacgg ctgaacagtt tggcttctcc
     2701 agagggatca cgagtgtcgg gaaaggctta atcatgaagc actaccgggc atacggtggc
     2761 cccacgccgc cgcccactgg ccatcagggg attgatgatg cgttcatcga gaagggttcc
     2821 cggacgtggt acttccacgg tggagagtgg ttgcagttga ccggagcgga ttgactgcag
     2881 gctctcggta gagcttcagt ccccttacag tcggaacgca caagattcag tgctcaaaag
     2941 aacgctgcac aattgtatac tgcgtgaagc cggagatgag aatggagatt ctgtctgttg
     3001 tctgacatcc tgacccgaag ttgctctccg gtcccggccc gtctgttaag tacctccatc
     3061 gaatccctcc taatgcttgt cttgctggcg cttgccgctt ccggccagca gcggctctct
     3121 gggattgcgg gcaccgtgta cgacacccgg ggcgccgtcg tgagcggtgc atcgattact
     3181 gtgaccggag tagatacggg cgtccgccgg gaggccgttt cgggcccgga cggcgtgtac
     3241 cgggttccct cgctcgagtt agggcgctat gcagtaaccg cctcgaaggc cggcttcaag
     3301 agcagccggc acgaagatgt gctgcttgag ctggagcggg aagcggtggt ggaccaccgc
     3361 cttgaggtcg gggacttttc ccagaccatc gtcgtggtgg gccagtcgcg actgatcgac
     3421 gcgagcgccg cgacgctgtc cggcctagtg agaagctcgg ctattgaaca cctgccgctg
     3481 aacggccggg attatacaag actggccctg ctgcaaccgg gaacggcagt ggcgcagagc
     3541 cagtcgaggg atgtcaataa cggctacggc gtgccgatca gcttcgcggg ttcacggccg
     3601 tttcagaacg gctttctcct cgatggaatc agcttgaaca gcttccacaa ttccgcgcct
     3661 gcgagcgtga atggggccac tctgggggtc gaggcgatcc aggagttcag cgtgattgcc
     3721 gggcctttca gcgccgagta tggacgctcg ggcggcggga ttatgaacgc cgtcacgcgc
     3781 tcgggcggca acgaatacca cggcagcgcc tactacttcc accgcaatga caatctggac
     3841 gcccgcaact tcttcgatac cgcgaagcct gagttccgcc gtcatcaggg tggcatctcg
     3901 gtgggagggc ccctccgccg caaccgggtc ttcttcttct ccaactacga agccttccga
     3961 gtggcgcgtg gcaacacgac cgtcaacaca acgctgagct cggaagcaag gcgtggagag
     4021 ctgctgtcgg gaaccatgac agtggattcg gccgttgaga aagccgccgc tctgtacccg
     4081 ctgccgaacg gaaccatctt cggagacacc gggctgtttg ttttcaccaa tcagttgcgc
     4141 gcacggcagg acatgggaac cggacgcatc gactggaatc cttcggacac ggaccgcgtc
     4201 ttcgcgcgat atagcgtgga tgacgggctg cgaagccagc ataccgattt tgccctcggc
     4261 cggacgacaa acatcagccg cgcgcaatct ctctcgctgg agcacacgca tctgtttgga
     4321 ccttcctggt tgaacacagc tcgcgcgggc ttcgccaggg accggacggt ccgtggggcc
     4381 agccgggcgg cggtttcggc cgtggatgac ccgtcgctgg cattcctgcc ctcgggcgga
     4441 tcggtcggaa tcatcgaggt aaccgggctc acgactctgc ccggtggcag cggcgccctc
     4501 gacgccgacc tccacgcgtt cacgagttat caactgtccg acgacgcctt gcgtaccggc
     4561 ggcaaacaca tgttccgctt cggcggacgg ctggaacgca catggttgaa cagcgacagc
     4621 cagaacagcg tgagcggcat ataccagttc aggagcattg ccgagtttct gaccaaccgg
     4681 ccaaaccgct tccaggcaca gttgccgggg tcggacaccg tgcgcggttt ccggcaatgg
     4741 accggtgcgc tgtacgtcca ggaccattgg cgcatccacg agcgggtgac cgcggaactg
     4801 gggctgcgct gggaggttgc cagcgtcccg gccgaggtgc aaggaaagct gtcaaatctt
     4861 gatcagctca cggacgcccg gatgcgcgag gggcaaccat ttttccgtca gccatcggcc
     4921 gtcttcctcg ctccgcgagc cgggttggcc tgggacacgc gtggggctgg gcggaccgtg
     4981 ctccgcgcgg gatacggcat ctacccagac ctgatcctga cgcagttcct gctgctggcc
     5041 gggatgagga accctcccta cttctttcgc gggagcctgc gcgctctgca gaaaggtgac
     5101 tttccgtcca acggctaccg gcgccttgtc gagagcggaa cggctgagct ccggatagac
     5161 cggttcgatc ccgcgccaaa tcaaccctac gttcaacact ggactctgca attggagcag
     5221 cgcctgagcc ggcttgcgac ggcccgggcc gcgtatctgg gctcgcacgg gctgaacctg
     5281 tcgtcagtca ccgatgatgc caatctcgtg actccggtcc ggctggcgga cgggcgcctg
     5341 tactatccgt ccgacgggaa gaaactcaac ccgaacttca gtgccatccg gaaccggctt
     5401 ttcgatgcgc actccttcta ccatgggctg ctcacttcgc tggagagacc gttggaccat
     5461 ggcctccagg tccaggcatc ctacacgttc tccaagagca tcgacgacag ctcgaacttc
     5521 ttcagcccga cggaatcgac gaactacagc tcctccgcgg tcatcgataa cccgagattc
     5581 aatcgaggcc tttccgcgca cgacgtgcca caccgcctgg cggtgagcgg catctgggag
     5641 gcgccgctcc ccaagcggac aaattggctg cagccccttc gcggtctggt cctgagcgcc
     5701 atgtacacgt attcgtctgg catccctttc agcgcgtggc tgggatatga cggcgccggc
     5761 acaaaggcgg agaggtcgga ttggcgctcg ggccagcgcc ccgacatcat cgagggatca
     5821 ggtcggcgtc tgttcacagg caacccggac ggctgggtgg acttgtcagc gttccgccgc
     5881 ccggagcgtg gtttccttgg aaatctggga cgcaacacca tccgcgggcc caatctttcc
     5941 aatctcgact tctcgacgaa ctggcggcgg cctcttaagg agcgggcgac cgtagagctg
     6001 cgtttcgagg tctttaactc gctgaatcgg accaacctaa gcattcctag tgcggagcgc
     6061 atgcagatct tcaccgtgga cggcatccgg gaggacgccg ggcggatcac cgaagccctc
     6121 gatagccgcg agatccagat cgggcttaag atcagtttct gatgctcgat tccctccaga
     6181 tgctgttgcg gtttagcacc ggactcagga agcacctctc ctcgccgctg acgatccagg
     6241 agtgccgcga catcgtcgtc aagagtatcc aggagcgaga agttagcttt ctggacctgc
     6301 tccggcgggc gatctacgag aatccccgca gcccctacct ggcgctcctc agcagggcgg
     6361 gcgccgagta tggcgacgta tccgccattg ttcggcagga cggcgtcgac gccgcactgg
     6421 agaagcttta cgatggcggc gtccgcatcc gcctggagga gttcaaaggt ctacgaccca
     6481 ttgagcggtc cggcctgaca ttggcggtaa aagcggaaga ctttgacaac ccccttctgc
     6541 ggcgcgactt cgaggtagcc accagcgggt ccacaggcag gcggaggcgc ctggccatcg
     6601 acgtcgacct cctgctccag gaagccgcgt atactcgcgt gatcgaggag gcccatggtg
     6661 ttttcgagcg cccgaaggca atctggcggc cagtgccgcc aggctcgtcc ggtttgaaag
     6721 ccgtgctcag ggccgagaag tgcggacgct ccctggacaa atggttcagc cagacgcaca
     6781 tttcctgggg gccgcggggg tggacttccg ctgtattcac cacctctgct gtgatgatcg
     6841 gccggttctg cgggaggtca atcccgatgc ccgaacacgt gcccttggat gaagcccggc
     6901 gtgtggctga gtggatgtcg gagcaagcct cggccggccg gcctgcctat ctgtcggccc
     6961 cttccagcac cgtagtgcga atctgccagg cggctcgcgt ggggggactg gatatcactg
     7021 ggagcgtatt ccgagtgagc ggggaacctt acaccgaggg caagcggcgg gtcgtcgatt
     7081 cctgtggggt acggaccttc agtggttgga gcttggccga gtgtggtggc gtggcgggag
     7141 ggtgcgcccg gagcgaggct ctcgacgaaa tgcatctgct gacaggcatg gtcgcgctca
     7201 tccagcggcc tgtttcattg cggggggagg cgggctctgt cgacgctttt cacctcacca
     7261 ctctccggcg tgagactcca aaagtcatgt tgaatgtgga cgccggcgac tacggcatgg
     7321 tctcccgccg acggtgcggc tgtccgctgg aagagctcgg gatgcatgtc catgtgcacg
     7381 gcatccgcaa ctacgagaaa ctgacctcca acggcatgca catcatcggt tcggacattc
     7441 tggagttggt ggaagacttt ctcccgcggc gtcacggtgg ggcgccgacc gattaccagt
     7501 tcgtcgagga atccggcggc gccgagagtc gcatcgccat tgtagtcagc aggcgcgtgc
     7561 cgaatctcga cgaaggggcg ttgctcactc acgtgacggc ttttctgacc ggcaagagcc
     7621 gggctcatcg catgatgatc aacgtgtggg agcaaggagg cagcttgcgc gtggtccgtg
     7681 cagagcctca cacgacatcc accgggaaga cacccgccct gcgcgtcata caggagttgg
     7741 aaaccgagac cggggaggcc aaacgcgttg gctcttgacg ccgcggtgca acgccgcagc
     7801 cggtggtcgt gggtgcgatc tctgtccggg ccggggctgg ttttcgtact cgctgctctt
     7861 ggtccgcaag atctcgtatc gaacgcggtg gccggcgccg atcacgggta ttccctgctc
     7921 tggacgctga tcctgcttgc catcgcgcgc tacaccgtgc tggaggcttc gggacgctat
     7981 gtggtggtat caggcgaaag cctggttgag gggtatgcgc ggtgcggccg ctggatcgcg
     8041 tggctgctcc tggtctcgat tctcatcaag cgccacctca cgaatctgtc actcattttg
     8101 ctgatgggcg tcgctgtgaa cctgttgttt ccactcgacc acccgcgtgg cgcggcgttc
     8161 tggtctctgg cgttgtggat gctgggtctc tgggtcatgt tgtggggacg gtacgcccgc
     8221 atcgagaagt ggttcagggg gctcgccata ctgctggcgt gttgcctgat cctggtcgcc
     8281 gcgctcgccc ggccggatcc gctcttgatc ttgaagggtc tgacgattcc atccatgccc
     8341 caaggcagtt ccttgccggg cacgctgttt gtactcatgg ctctgatggg tgcgggggcg
     8401 ggatcggtga acaatttgaa atacccggca ttcttgctgg agacgggatg ggacagtcgc
     8461 cactacctgc gcgccaaccg ccggcaggcg cttttcagct ccggcggcct gttgttggcc
     8521 gcggtcctgg tgcaggtagc ggcctccgcc agcgggcagc aagcggaggg aattagaacc
     8581 gccggggatc tgaccgccat cttcgggaga gccctgggcg agccgggccg tctcgcgatt
     8641 accgtcggac tggcggcagc ggttttctcc accttcgtcg gagccaacac agggtacagc
     8701 ctgatcgttt cggacctcgt ccacaacgtt ctccgccgga atcagagcag gacgggaact
     8761 cggccgcccg ggagcctccc cgcttaccgg tgggcgctgc tggtgttcac cgttccatcc
     8821 ctgtacgttc tcgtcaccaa ctggcagccc gtctggatgg tgctactgtc agcagcagcc
     8881 accgtcgcgt tactgcccct cactgttccc gtgttgctga tcctgaccag tgatcgcgag
     8941 cggatgggcg agcaggcgag cgggtgggcg acgaagatca taatgttgct cgtggtcctt
     9001 gccgccctct acctaacctg gcagaatgct cgaactctgc ttgccggcgg attctcctcc
     9061 gcgccgggct aggcgacgcg cagttgacgg ctgttgatcg gctcgggatg tccccctgtc
     9121 gtcggcttcc actctccagc tcgccgctgc gcctcagcga tctgagcggg agtcatcaca
     9181 ccagcaaacg cctcacgcag atcggtatat cgcttttggc ggctgccact ggcgtgtgaa
     9241 gccgccaggt taatccacaa atgtgcttcg acgtagtcct gtgcaatccc cccacccaac
     9301 gcgtacatga gaccgagagc gacctgcgcg ggggcatacc cctgcacggc tgccttgcgg
     9361 taccagtggg cggcttcggc gacgtcttgt gccatcccct gccctaagtc atacctggca
     9421 ccgagcaaga actgcgcgac ggcgtcgccc cgttccgctc gttcgcgcaa ggactttggc
     9481 gacttcgcag tgggagcctt cccctgccgg ccaaccctac gcaagagaca ccacaggctc
     9541 aataagagac ctaccagtcc gtatctcatc agaacgccca tcctgagccg ccgctgttga
     9601 cttaagtata ggagtgtcaa cgtggcgtgt cactccggta cgtggcggca cgtccgggag
     9661 gagccactcc cgggttcctt tcaggcggct ggatctccgg gcgggccagt ggcccaagct
     9721 gcactacact ggacgggatg aggtgaaaaa tcgtgactca gttgagggga gacagaatca
     9781 tgaatagcaa ccctttgagg cggttgaaaa cactggggca atccatctgg ctggactaca
     9841 tccggcgcga cttgatcacc agcggggagc tccggcgttt gatcgaggaa gatggcgtgc
     9901 gggggatgac ctcgaatcca gccattttcg agaaggcgat tacaggcagc cgcgactatg
     9961 acgacagcat cagggctctg gcgcttgacg gaaagggcgt ccaggcaatc tacgatgtcc
    10021 ttaccatgcg tgacgtgcag agtgccgccg acgagtttcg gccgctgtac gacaggactg
    10081 gcggcaagga tggatatgtc agcctggagg tcaatccaca cctggcgcgc gacaccaagg
    10141 gcacgatcca ggaagctcgc cggttatggg acagcttgaa ccgacccaac gtcttcatca
    10201 aggtcccggc tacggccgaa ggactgcccg ccattcagca gctcatcggc gaaggcatca
    10261 gcgtcaacgt gacgttgctc ttcggattgc cgcggtatcg gaaggtggcg gaagcataca
    10321 tcgccggcat cgaagcgcgc gccgcgcaag gaaaggccct gaatcatgtt gcttcggtgg
    10381 ccagtttttt tgtgagccgc attgatgggc tggtggaccc catgctggag aagcttgcca
    10441 cccagagtgg tgaaaaggcg gagctcgcca agaatttgca cgggcaagtc gccatcgcca
    10501 acgcgaagat ggcttatcaa atctacaaag agatctttgg ccacgatcgg ttcaaacgat
    10561 tggctggcag tggggcgggc gtccagcggc tgctctgggc cagcaccggc accaagaacc
    10621 cagactacag cgatgtgaaa tacgtcgagg ctctgatcgg gctggacacg atcaacacgg
    10681 tgaccatcga gactctcaac gcataccgcg accacggcga cccgaaagcg cggctcgaac
    10741 aagatgtcat ggaagcccgc tgggtgctgg agcaactgcc ggagctgggc atcaacatcg
    10801 acaatgtgac gcaacagctc gaggacgaag gagtccagaa gttcaaccag ccgttcgaca
    10861 agctgatgga gaccttggcg caaaagtcgt cacagacttg acggtgagac atgaaactga
    10921 gctctcttgc gggaaaaccg gccgaggcat cgatgctggt gaacgtgccc agactcgtga
    10981 cagcctacta tgcggagacc ctgacccttc ggtgcctgga cagcgggtct tgttcggcac
    11041 gtccggacat cgtgggtctg cattcgacaa ggctttcaac gaatggcaca tccccgccat
    11101 tagtcaggct atctgcctgt atcggaagca gaaagaaatc gatggtccac tgtttctcgg
    11161 cacggacacc accctggagc tgatctaccg cttcaagcag cggctctgct acctgctgct
    11221 gaaaaaacac cgcacccgca aacagtgcca agcgctcgtg cctcgcttcc tgcgtgccgt
    11281 gtaccagttg cgctaggccc gcctggcgca actggttcaa ctcggcgaaa ccttgtcctc
    11341 cttatagtcc cctcaggagg agaatcgaga tgaagggttt tccacagtcg ccgccgccag
    11401 aaactgtgga aaactggggt tgcgtcgcga tcatattgac gctcttgctt ccgcgcatcg
    11461 tttgcgatac gtgagagagg agcgggcaac cccgctgcta cgactaatgt cctcccgtac
    11521 gcaaaacgtc tggaccagag tgtcgagcag cgggttgcgc cttgtttcca aacgaatact
    11581 cagatgtcgt ctggtttcga caacccgact ctactataag gctttcgccg agacaaggcc
    11641 ctgcccgtga aatcgaaggt cgtaaagagg agatgagcat gtcggatcta ccgaatagac
    11701 ccgggccgga gtttgcggcc tggatcggga tcgattgggg cgacaagaag catgcctggg
    11761 cagttcaagc cgagggttcg acgcaagtcg agcagggcga gatggcacac acgcccgagg
    11821 ccatccagga gtgggccatg gagctgcgac ggcgattccc caccggccgc ctggctgtcg
    11881 ccctggagca atcgcgcggc ccattggtgt tcgtcttgag taagtacgag cacttggtgc
    11941 tgtaccccat tcatcccacc agcgctgcca actatcgaaa gatcttccgg ccatcgggcg
    12001 ccaagagcga tggcccggac gcagcgatac atttggacat gctggtcaag catcgggacc
    12061 agcttcggcc gctccatcca gacaccgtgg agactcgcac actgcaactt ctggtggaag
    12121 atcgtcggca gttggtgaac gaacggacgc gcatttccaa ccgtctggtc gccgatctga
    12181 agtcctacta cccccagccg ttgcagtggt ttgaaaaacc ctacgcgccg gtcacgctcg
    12241 atttcctggc gcgctggcca acgttggagg aactgcagaa agcccggccg gcgaccctgc
    12301 aagagttctt caagcaacac cagtgccggg acgatgcgaa gaaccaagca cgcatcgaag
    12361 acatgcacaa aaccatagcg gcaacccacg atggcgccgt gaggatcgcc ggccgcgcgg
    12421 cggtgtgcgc cgcggtgcgt ctgctccgcg gcttgagcca gtccatccag gattacgatc
    12481 agaagatcga agagttggct caggcccatc ccgatttcga gatcttcgtt tcgctgcccg
    12541 cagccggtgc cgcgttcgct ccgcgtctca tcgctgcctt cggcgcccag cgcgatcgat
    12601 accgggacgc ggccgaggtg cagcagttta gtgggatcgc cccggtgacc gaagccagtg
    12661 gcaaccaacg ctgggtccac tggcgctgga gttgtccgaa atttctcagg cagaccttcc
    12721 acgagtgggc ggcacaaacc attcaacagt gcgactgggc tgcggcttat taccaagtgc
    12781 aacgcgagga caagaagaaa tcgcaccacg ctgccgtacg gtccctggcc tacaaatgga
    12841 ttcgcattgt tttccgctgc tggaaggacc gaaagcccta cgacgagtcg cggtacgaag
    12901 gtgctctcaa acgccaacac cagaaagccg ctgccaagtc ggtccaaccc tccccgtttg
    12961 agttgccggt ccagtggatt tcctgtgcag gcttccaaaa actcgcttca aaaaatggat
    13021 aaaaaccctt gactccagac ctcagatgtc tggtcagagg agatcgttgc catgtggcgc
    13081 ttcacccgca acaacggcat caccgaaggt tttcacaaca aaatggagct cattaaccag
    13141 caagcctacg gcttccgtaa cttccaaaac tacagacttc gagtcaaggt attatgtggg
    13201 tgattcaatc gggagcgggg cttgcccccg tttttggcgt agagcccatg agtaaacaaa
    13261 gtctggcgga gagggaggga ttcgaaccct cggtagagct ttatggccct acgacggttt
    13321 agcaaaccgc tgctttcgac cactcagcca cctctccgcg gtctggtggg tctcttgcat
    13381 agtatcatag caggcccggt gcgttaccct ctcgcttgac ggctcggcac ggccgcggta
    13441 cagttgatat gggaccagaa gggtcgcata tccgaaacat gctgcggctg accaagaagg
    13501 ctgattatgg actgatcgca gtgacccatt tggcagccgc gggcgaaagg ggggccgtga
    13561 gcgcgaaaga aatcgccgac gcctatcaca tcccgctgcc gctgctggca aagatcctcc
    13621 agaaactcgc gaggcgaggt gttctggctt cggtccacgg aaccaacggc ggctaccgat
    13681 taagccggga cgccagtcgt atcagtgctc tcgaagtgat ccgcgcgatc gacggtccgg
    13741 tagccctgac ctcctgcgga ggctcccacg gagactgcgg ccagtccgaa aagtgtaccg
    13801 tccgcgagcc gttgcgggtg gttcacgacg gaattatgga gctgctggcg ggtatcagca
    13861 tttccgatct ttcgcaggac cgccggcgag ttccactgac aatgatccag acatcatgag
    13921 gcactcatca tgacaaagcc gatctatatg gacaaccacg ccaccacgcc gatcgacccg
    13981 cgcgtgctgg aggcgatgat gccttacttc accggtaagt tcggcaacgc cgccagccgc
    14041 aaccacagct acggctggga ggcggagcag gcggtggaaa aagcgaggca gcagatcgca
    14101 aggctcatcg gcgcctcccc gaaagagatc gtcttcacca gcggcgccac cgaatccgac
    14161 aacctagcca tcaagggcgt ggctgggatg tacgccgaaa agggcaacca tatcatcacg
    14221 gtaccgacag aacataaagc tgttcttgat acctgtaaac ggcttcaaaa gcacggctac
    14281 gagatcacct tcctctcacc gggacgctac ggcattatcg atctcgacga actgcggggc
    14341 gcgatcacgg acaagacaat cctgatcacg gtgatgcacg ccaacaacga gatcggtgtg
    14401 ctgcagccga tccgcgaaat cgggaagatc gcgcgggaac ggggagtttt gttccatacc
    14461 gacgccacgc aatcggtcgg caaggttccg gtcaacgtca atgacgacaa cgtcgacctg
    14521 atgtccatca gcgcccacaa aatgtacgga ccgaagggcg tcggagcgct gtacgtgcgc
    14581 cggaagaatc cgcgggtcca gctcacctgt caaatggacg gcggcgggca tgagcgcggg
    14641 atgcgctccg ggacgctcaa cgtgccgggc atcgccggcc tcggcgaagc gtgcgcgatc
    14701 tgccagcagg agatggcgaa agaagccatt cggctcagcg atttacggga caaactgaag
    14761 gcaaaactcg aaggcggtct cgacgaggtt ttcgtcaacg gctcgatgga acatcgcctg
    14821 ccgaacaacc tgaacatgag cttcgcctat gtcgagggcg aatcgctatt gatggggatt
    14881 aatgacgtgg cggtttcgag cggttcggcg tgcacgtccg cgacactgga gcccagctac
    14941 gtgctgaagg ctctcggggt aggagacgac cttgcccata cgtcgatccg cttcgggctc
    15001 gggcggttca acaccgagga agaggtcgac tacgtggctg cgcgggtgat cgaggtggtg
    15061 aagcgacttc gggaactatc accgctctac gaaatggcta aagagggcgt ggacctgaag
    15121 acggtggact gggttgcgga gtgaggaatc gcggcacggc gccgccgtat ctgatgaggt
    15181 agagagggaa cgatggcata ctcggacaag gttttggacc attacaacaa cccccggaac
    15241 gtcgggacga tggacaagaa ggatccgagc gtcggaaccg gcatggtggg cgcgcccgag
    15301 tgcggcgacg ttatgaagct ccagctcaag gtcaaccccg agacgggcgt catcgaggac
    15361 gccaagttca agaccttcgg ctgtggcagc gccatcgcca gctcgtcgct cgccaccgag
    15421 tgggtgaagg ggcgtacggt ggaccaagcc ttggagatcc ggaacacgga cattgtgaac
    15481 gagcttagcc tgccgccggt gaagatacac tgctctgtcc tggccgagga cgccatcaag
    15541 gcggccatcg acgactataa gcgcaagcag gaggctctga gagcagccga accggcgatt
    15601 tcctagaata cagtcaggag ggggaggagt cgaatgattc aagtgacgcc aaaggctctg
    15661 gacaaaatcc gccaggcatt cacgcgccac ggcgtgagcg aggccggagg gctgcgcctg
    15721 ggggtgcagg gcggcgggtg ctccggcatg agctacctgt ttcgcttcga tccgaaggaa
    15781 cgcccaaacg acaatgttta caacttcgag ggtgtcagga tcttcataga ccctaagagc
    15841 ttggtctacc tcaagggaat gactctcgac tacaaagagt cgctcatcca ctcgggcttc
    15901 gtgttcgaaa acccgaatgc aaagaaaagc tgcggctgcg gcacgtcctt ctccgcctga
    15961 tgcacgacgc agagagttgc tggaaatgcg gtgagccgtc cgcacagtca cttttctgcc
    16021 gcttctgtaa tacgctccaa gctccgtctt ccgactatta ccggtttctg gggctggagc
    16081 ggcgtctaag cctcgatccg gacgacctcc agcgacggtt ttacagcctg agccggctgg
    16141 tccaccctga ccggtatctc cgaagcgcag cgaacgagca acgctttgct ctcgaagcca
    16201 ccgccctact caacgatgca taccgaacac tccgagatcc ggtcgcacgc gccgagtact
    16261 ttctgaaaga agagggcttc gacagcggag gacaaaagtc gaacaacgtt ccgccggaaa
    16321 tgctggagga agtgtttgat ctgaacgagg cgctggagga acttcgcggc ggtgatccgt
    16381 cggtgcgtcc ccaactcgaa gcggctcgtc ggcggtttcg gtctcttcgg gaggacgtcg
    16441 acggcgacct tgaggcgcgg ttccgtgagt acgatcgggg ccggcagcgg gaaatcctgg
    16501 gaaagattcg ggcgctgctg aatcggcgcc ggtacatcga gaacctgctt cacgatgtgg
    16561 agcgcgagtt gatgaactag gccatgtccg acacgtttca gattgatttc agcgcgagtg
    16621 atcgtccgct ggtcgtggga atcgacctcg gcacgaccaa cagccttgtg gcgttcatgg
    16681 acctcactgg tccccgtgtg attcccggag acgacggaaa caacctcgtt ccaagcgttg
    16741 tcagctacag ccgggctggg gagatcgtgg ccggcagact tgcgcgggag atgttgatca
    16801 ccgaaccgga gcggactgtc tattccgtga agcgcctcat gggccgcggc gtcgccgacg
    16861 ttcaggagga actgaagctc tttccgtttc gcattgcgga gggcagtgag tcggtgatcc
    16921 ggctggaact cggcgagcgg aagttcacgc cgccggagat atctgcattc atcctccgcc
    16981 aactcaggca gaatgccgag gcggacctcg gtgagacggt gaccaaagct gtgatcacgg
    17041 taccggcata tttcaatgac gcccag
//
LOCUS       DATIFQ010000082         8680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2325367, whole genome shotgun sequence.
ACCESSION   DATIFQ010000082 DATIFQ010000000
VERSION     DATIFQ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325367"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(424..1263)
                     /locus_tag="VLE22_03575"
     CDS             complement(424..1263)
                     /locus_tag="VLE22_03575"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSB13510.1"
                     /translation="MSALDLARLVDPGAKPKRIATGFRFTEGPVFSRLNYLLFTDIPA
                     SRIMKWSQGAVTVFRENSNGANGLTFDHQGRLLACEIGQVTRSEKDGSLTVLAARYDG
                     KGLHRPNDLVYAIDGSVYFSDRRSKTAPGGLPMTELSAVYQVTRSGEVRIAARDCDLP
                     NGVALAPDQQKLYVADRNLLRVYEIAADGSLRSGRVFADMQSGAPGGPDGLKTDESGN
                     VWVAGPGGIWVFDPKGERLGIIEVPETPSNCCWGDAFRNLYITARTSVYRLETKVSGT
                     RTY"
     gene            complement(1260..2456)
                     /locus_tag="VLE22_03580"
     CDS             complement(1260..2456)
                     /locus_tag="VLE22_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13511.1"
                     /translation="MRRREFLQLPALAVARPPGRKKIAALVTTYHVRSHADNFVTRFL
                     EGYWINERYTAPPCDIVSLYMDQVHPADIGRRLASAYGFPVVKSIPEALTLGTGRLAV
                     DGVLIVAEHGNYPDNEKQQKLYPRFEFFEQVVQVFRKSGRSAPVFTDKQLSYDWSKAK
                     QMYDWSRELRFPMMAGSSVSVTFRRPELDYPLDTVFEDALMVGGGWIADGGIFHNLET
                     LQCFVERRKGAETGVRAVHHLEGEAVWKAAEQGLWSKELMLAALARAEKSGPGRPEDV
                     KQPVLCLLEYNDGFRAAVVMLGGLVNEYLVAFKTKGRNGIDSTLCYCPAENSNNFSPL
                     VDAIARMYLTGRTAVPVERTLLTTGALSFLMESAHQGHKRIETPSLKIAYRASPSSYY
                     AHGRGS"
     gene            3338..4192
                     /locus_tag="VLE22_03585"
     CDS             3338..4192
                     /locus_tag="VLE22_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB13512.1"
                     /translation="MPFKMTMSTDNWRPDWWGVERCFQRIRELGERHVELTTATGFNL
                     LEGLGFSPYISVDDDPFIIRDLLQKYGLELVSIDCDYPIWSHHSIDVLIKSIVFGDMI
                     GCRLFVTTDSGNYPPGRSDEEWLRVIKYHFDCVLPVAERHNAVIAIEPHGYLTTKPDA
                     LWRLATQNASDRIGINFDTGNSFIAGQDPVAFLGKVKDRVVHMHIKDVSEALARAMGG
                     EETGIASSEIAVGEGVNAENIMLCLDIMAATGREIPISPEAGGDRLMPRSIAWVRDYL
                     NRKGYALV"
     gene            complement(4364..5473)
                     /locus_tag="VLE22_03590"
     CDS             complement(4364..5473)
                     /locus_tag="VLE22_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB13513.1"
                     /translation="MNINRRSFLLGSAVTARIGYGQTAQTVNTASIGVGNRGSYLLKA
                     VLAQPNAKVVALCDIKPDRLDKAASAAARDNPATYADWRRIIDRKDVEAVFIATPPHL
                     HSEMAIAALEAGTHVYCEKPIGVTAAHVRDLVKAARSTSKVFVVGQDMRTEGQLAEAI
                     RKIREGAIGDVIMVKAQRHASSDLNHAGSSADWYFDVTKSGGYLIEQSVHNLDACNWV
                     IGAHPSRACGFGGTLLYKNDPPGRTIFDCGLLTFDYPNAVKMSFTQNVFHPRRMPNNN
                     QMIYVYGTKGAVDLLDTVMMYPLARDAQPSLLAEKRQDPPHAHVTAFYDCIAKGAPNP
                     ADITVGATAALTSILGHEALVTEKVVNWADLGVDL"
     gene            5472..5606
                     /locus_tag="VLE22_03595"
     CDS             5472..5606
                     /locus_tag="VLE22_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13514.1"
                     /translation="MITSGSVYAEEAAYGFHPPSLTMVVTSSRISMDRQLRDIDLEVR
                     "
     gene            6868..7140
                     /locus_tag="VLE22_03600"
     CDS             6868..7140
                     /locus_tag="VLE22_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13515.1"
                     /translation="MPKHRLLDAGILSAALEGLELQKQRLEQKIAEVRRVLGGVTRKA
                     AVVAVEAKRPRRKMSAAARKRIAAAQRKRWAEWRKKGRTAGAKAKG"
     gene            complement(7315..7968)
                     /locus_tag="VLE22_03605"
     CDS             complement(7315..7968)
                     /locus_tag="VLE22_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB13516.1"
                     /translation="MRISTSPEIPSHLTVLFCSVMRSAPVGVAVCVFAMCAALAPAAM
                     MTDLERQRLVAHLEMTGSWLGDEISGLSLAQLQFRPAPGSWSILEVLNHLVVSEPIYW
                     RNFQDAMNAPPVNRGPSGSDDSVLWYGIDRTNRQKVAVGEDATGKLKEVKTGLAAFRK
                     LRAEMLHYARTTKEDLRAHFVERERSDAYQWFLLISTHSQRHILQIREIKANLSFPR"
     gene            8147..>8680
                     /locus_tag="VLE22_03610"
     CDS             8147..>8680
                     /locus_tag="VLE22_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13517.1"
                     /translation="MDSLAHGLWAAAEATVAKRTAGLRVRILPFALWATFPDMLAFGP
                     GIAVGLWLRIAGGAGYAESAHGGHFHHFHVGLPLYAMGHSLLVFASVYGLCSLVARRG
                     VVSPLGWLLHILIDIPTHSYSYYATRFLWPLSNFGFDGVAWWTPWLLWCTYGSLALVY
                     LLMWRKGWLRSTSRSSSR"
BASE COUNT         1716 a         2599 c         2627 g         1738 t
ORIGIN      
        1 gtggtcagaa tgaaccagaa gacattgcac atgggcggat ttctcctccg gctgcacagc
       61 acctcatggg aatcaggtcc atgctctgtt ttacccagga tttccccact ggctgcctgg
      121 ccacggtcga ttatatgccc agcggcctcc atccccgatg ccagcgctca ttctgcgggt
      181 cattacgggc ctggaaagcc gctccataac tcggattcgt acgatctcgg tgcggtcgag
      241 gaagacctgt ggttcggcag cttccccccg attcgcccca agaaccctca acggtcaggg
      301 gaggtgcggt cgccgagcgt ccgcagcagc cgctgcacgg tcatggcgcc gacgacaacc
      361 gcaactcgcc tgcgctcggc ctgaaccgaa gttaactgta agtggtcaaa gtggagaagc
      421 gggttaatac gttcgtgtgc cactgacctt cgtctccagc cggtagacgg acgtccgggc
      481 cgtgatgtag aggttgcgga acgcatcgcc ccagcaacag ttgcttggcg tctcgggcac
      541 ctcgatgatt ccgagcctct cgcccttcgg gtcgaatacc cagatgcccc ccggtccggc
      601 cacccatacg tttccgcttt cgtccgtctt gagaccgtcc ggaccgcccg gtgcgcccga
      661 ctgcatatcc gcgaagacac gtccgctccg aagcgagcca tccgcggcaa tctcataaac
      721 gcgaagcagg tttctatcgg cgacgtatag tttctgctga tccggcgcga gagctacccc
      781 gtttgggaga tcgcagtctc gcgcggcgat acgcacctcg ccgctccgcg tgacctgata
      841 cacggcggag agctccgtca tcggcagacc tcctggtgcg gtcttcgacc gccggtcgct
      901 gaagtacacg ctcccatcaa tcgcgtaaac caagtcgtta ggacggtgaa ggccctttcc
      961 gtcgtacctg gctgccagga cggtgaggga accgtccttt tcgctgcgcg ttacctgtcc
     1021 gatttcacag gccagcagcc gcccctggtg atcgaaggtg aggccgtttg cgccgttact
     1081 gttctcccgg aacaccgtca ccgccccctg cgaccacttc atgatcctcg atgcgggaat
     1141 atcggtgaac aacaggtagt tgagccggct gaatacgggc ccttccgtaa aacggaagcc
     1201 ggtggcgatt cttttcggct tcgcacccgg gtccacgagc cgtgcgagat ctagcgcgct
     1261 catgatcccc tcccatgcgc gtagtaggaa ctgggcgacg ctctgtaggc gatcttcagg
     1321 gacggcgtct caatccgctt gtgtccctgg tgagcggact ccatcaggaa cgacagcgca
     1381 ccggtggtga ggagagtacg ttctaccggc acggcggtcc ggcccgtcag gtacatccgt
     1441 gcgatggcat ccacaagcgg gctgaagtta ttggagtttt cggccgggca atagcagagg
     1501 gtgctgtcaa ttccgttccg tcccttcgtc ttgaaggcga cgagatattc attgacaaga
     1561 ccgcccagca tgaccacggc ggcgcgaaaa ccgtcgttgt actcgagcag gcacagcacc
     1621 ggttgcttca cgtcctccgg gcggcctggg ccggatttct cggcgcgtgc cagtgcggcc
     1681 agcatcagtt ctttcgacca caggccctgc tccgccgctt tccagactgc ctcaccttcc
     1741 aggtgatgaa cggcccgaac tccggtttcc gctcctttgc ggcgctcaac gaaacactgg
     1801 agcgtctcga ggttgtggaa gatccctccg tcagcgatcc aaccgccccc gaccatcagc
     1861 gcgtcctcga agacagtgtc gagcgggtaa tcgagctccg gccgccgaaa cgtcaccgag
     1921 acggaagagc ccgccatcat cgggaaccgc agctctcgcg accagtcgta catctgcttc
     1981 gccttggacc agtcgtacga taactgcttg tcggtgaaca ccggcgccga gcggcccgac
     2041 ttccggaaga cctgtaccac ctgttcgaag aattcgaaac gcggatagag cttctgctgt
     2101 ttctcgttgt cgggatagtt tccatgctcg gcgacgatga ggacaccatc gacggcgagc
     2161 cggccggtcc cgagcgtcaa cgcttccggg atggacttca ccaccgggaa accgtaagcg
     2221 gaagcgagcc ttcggccgat gtcagccgga tggacctgat ccatgtagag cgacacgatg
     2281 tcacaggggg gcgccgtata ccgctcgttg atccagtaac cctcgagaaa ccgggtcacg
     2341 aagttgtcag cgtgggaacg gacatggtac gtcgtcacca gggccgcgat cttcttccgg
     2401 ccgggtggcc gggccaccgc taacgccgga agctgaagaa attccctgcg tctcatttgt
     2461 tggaaatagc ctcattggtg cgcatgatcc ggagcaggtt cccgcctatg accttgcgaa
     2521 tgtcctcatc cggtcgacgt cctcgagtcc ggccagcgcc atcccgagat gccgtcgaaa
     2581 tcctagccga taccgacgag taccgggccc gcgaccttgg cgatgtggtc gatgtggcgg
     2641 agggccgcct tgatatccac cttcggaaac tgggagcgat agcgcagcgc cgctgaatgg
     2701 cgcgcacgtc gctgctaggc aagatgagga ctcctaactt ggcggagtac acggcaactt
     2761 ccgtattggc cttgcggccg gtatcagcgt agcacgatcg gcgtgtcgtg gagccgttgc
     2821 cgctcacctt cttctcctga atgccttctc tttagcgccg cgagggtcgt cctgctcctc
     2881 ccgaggaccc cgtggccagt aactgcgtca ccgcacccgg ccggatgcgg cgccctggga
     2941 gcgggtgggg gcggttctcg ccgtgcgaag caccaggtgg agaaggcgat catcaaccgg
     3001 cgcaaaaggg cgcgcggcac acacatcaac aattttttcg accccccgat cgcgtgtata
     3061 cttatgtgcg cggaaagatc ctacgtggcc aggaggcttg agcgggagtc ctgggtaaga
     3121 cctggcagca attgccagtc cgcatctaat cgattgattt ccggagctgc ctggttcggg
     3181 cattgtgccg ttttcggaag aagatgtgcg aaatcggacg atgctagggc gaaacgcgag
     3241 tctcggaagc cgggatacgc gtccttgttc ggttcgcgat gttgaaacag cagccttggc
     3301 gaggtgaggg acgagaaaag aaaggacgga atcatccatg ccattcaaga tgaccatgag
     3361 caccgacaac tggcgcccgg attggtgggg tgtggaaaga tgtttccaac gcattcggga
     3421 gctcggcgaa aggcacgtgg aactcaccac cgccacgggc ttcaacctgc tcgagggctt
     3481 aggattctcc ccctacatta gcgtagacga cgatcccttc atcattcgcg atctcttgca
     3541 gaaatacggc ctcgaactcg tctccatcga ctgcgactac ccgatctgga gccatcactc
     3601 catcgacgtg ctgatcaagt caatcgtatt cggcgacatg atcggatgcc gtctgtttgt
     3661 caccaccgac agcggtaact acccgcccgg cagatccgac gaggagtggc tccgcgtcat
     3721 caagtaccat ttcgattgcg tgcttccggt ggccgaacgg cacaacgccg ttatcgccat
     3781 cgagccgcac ggctacctca ccacgaagcc cgacgctttg tggcggctgg ccacccagaa
     3841 cgcaagcgat cgcatcggca tcaacttcga tacgggcaat tccttcatcg ccggccagga
     3901 tcctgtggcg tttctgggga aggtgaagga ccgcgtggtt cacatgcaca tcaaggacgt
     3961 cagtgaggcg ctcgcccgcg cgatgggcgg cgaggagacc ggcatcgcct ctagcgagat
     4021 cgcggtcggt gaaggggtaa acgcggagaa tatcatgctc tgtcttgaca tcatggcggc
     4081 caccgggcgc gagatcccca tttcgccgga ggcgggcggt gaccggctga tgccccgcag
     4141 catcgcgtgg gtcagagact atctgaatcg caagggctac gcgctggtct gagccacgcg
     4201 gcggccactt ccttttcctc gcatgaaact tcgatgccag gcgtgcgcct cggcagtcca
     4261 aaggctacga ccgtaagcgc tgattccaca ccaccgccag gttccgccat cggacagcca
     4321 ttcgccagcc gaagtacgat ctgtgatgta gccgtggaac gcgctacaga tctacgccga
     4381 gatcggccca gttaacgacc ttctccgtga ccagcgcctc gtggcccagg atcgacgtaa
     4441 gggccgccgt cgcaccgacg gtgatatccg ccggattggg cgcacccttt gcgatgcagt
     4501 catagaaggc cgttacgtga gcgtgcggcg gatcttgccg cttttccgcc aacagggacg
     4561 gctgggcgtc tcgcgccagg ggatacatca tcaccgtgtc cagcagatcc actgcgccct
     4621 tggttccgta aacgtaaatc atctggttgt tgttcggcat ccgcctggga tgaaacacgt
     4681 tctgagtgaa cgacatcttc accgcgttcg gatagtcgaa cgtgaggaga ccgcagtcga
     4741 agatcgtgcg ccctggagga tcgttcttgt acaacagcgt cccgccaaaa ccacaggcac
     4801 gcgaaggatg cgctccgatc acccagttac acgcgtccag gttatgcacc gactgctcga
     4861 tcaggtagcc gccggacttg gtcacgtcaa aataccagtc ggccgatgag ccggcgtggt
     4921 tgagatcgct ggaggcatgc cgctgggctt tcaccatgat cacgtcaccg atcgctcctt
     4981 cacggatttt ccgtatggct tccgccagtt gcccttcggt ccgcatatcc tgcccgacaa
     5041 cgaatacttt gctggtgctc ctggcggcct tgacaaggtc acggacatgc gctgccgtga
     5101 ccccgatcgg tttttcgcag tagacgtgcg tgccggcctc gagagccgcg atggccatct
     5161 ccgagtgcag gtgcggcggc gtagcgatga aaacggcctc cacgtccttc cggtcgatga
     5221 tcctgcgcca gtcggcgtac gtcgccgggt tgtcccttgc cgccgcgctg gccgccttgt
     5281 cgagccggtc gggcttaatg tcgcacagcg caacaacctt ggcgttcggt tgcgcgagta
     5341 cggccttgag caaataggac ccgcggtttc cgacgccgat tgatgcggtg ttgacagtct
     5401 gtgcggtttg accatagccg atgcgtgcag tcacggccga accgagcagg aaggatctgc
     5461 gattgatgtt catgatcacc tctggatcag tctacgccga agaggcggcc tatggtttcc
     5521 atccgccgtc actcactatg gttgtcacct cgtcgcggat ttcgatggac cggcaacttc
     5581 gtgatatcga tctcgaagtg cgctgaccgc cgaaaatctg tttccgtcga aaacgactcg
     5641 cgctgttcca ggaacgcaag gtcaaacctc acggtgccgc cgacgcgaca aggctcatga
     5701 tggtcctcct cggccgattc ttaactgagc ggattggtga agccttcggg cgctttgtgg
     5761 agcttccggc cccaagagcc tggatcgaag aagactgagc cacgtgcgag gaagagtcct
     5821 ccaaacgata gatggcttcc gaggtcacca cccgactgga atccacagcc gacgcttcag
     5881 aaataagcgc tggaggacta cctacaggtg atcgggtcgc aagtgctgtg atagtctctc
     5941 acagacgctc aatttcctcc tgctcgcctg ctgctaccag gaggtctcgg agcttcgggc
     6001 ctgaactctc ggcatagcct caggacaacg gcggaaaact cagcagacac cgacggaatc
     6061 ctgcgccgat tcaaccggct gatccgagaa ctgctccaag ggggagcccg ccgggagtgg
     6121 ttccagcgtt ggaaagtgga gctgctcctg gacgcaaccg caggaacaga ccgtgtggag
     6181 gagaaaacgc tggagtggac tgtgtggccc aaaggacagg aataacgcga ctatgaaccc
     6241 ggtcatataa atggtctcgt aactgagcga ctcgattctc gacgttgccc tcatcaaaga
     6301 cctcctgtgt taacgtcctg aaacggcaca ctcaaattct cttcgagttc cctcagttcc
     6361 cgatcgatga tgtgttgctc aatggcccat ccaagtccgg gacagcctgt caaacgcctt
     6421 tttgactgaa tggtttggag aaggctaaac ccagattcaa gtctcgcccg gagagtttgc
     6481 tccctagcag cgttatcgca aagcatcatc gctgtccttc ttctaatcct acattgagag
     6541 cacgagtaaa gatgttaaag cccggaaaag taaatgtaag aatgatcgac cacccgatcg
     6601 gcctcgtggc aaactgacgg cctgagttca gaactgtgag atcaagaaat cggcccgggg
     6661 agacaccctg cgtcgctacc agcgggcggt tcagcgtcag cttgagcggg gtggcacggt
     6721 accgatgaag ttgtccgagt tccttgcgtc caggcgttag tggatgtcct gaggccgctg
     6781 ttgccgctgc ggatgtataa tcgctgtctc ggtccgtgca gatcgttcgt cccgaccatc
     6841 agaagataaa ggagccagtg taaacccatg ccgaaacaca gattgttgga cgcggggatt
     6901 ctgtccgccg cactggaggg gctggaactt cagaagcaac ggctagagca aaagatcgcg
     6961 gaagtgcgcc gcgtgcttgg cggcgtaact cggaaggctg cggtggtggc agtggaggcg
     7021 aaaagacccc ggcgcaagat gagtgcggca gcgaggaagc gcattgccgc ggcgcaacgg
     7081 aagagatggg ccgagtggcg gaaaaagggg aggacagcgg gcgcaaaggc gaagggatag
     7141 tccgccaccc tcggctacga acgccaagca gcatgagaag taccggcacc gggctcgctg
     7201 ctggtacgaa cgtgctgccg gcagcgcgaa gccgcgtcac accgacgggc gcgctggtcc
     7261 gtcccgaggc gtcgagcgtc ggtgacgctg ctctccgccg cacgcgatca gctactaccg
     7321 ggggaaactg agattggctt tgatctcgcg gatctgaagg atgtgacgtt gcgagtgtgt
     7381 ggaaatgagc aggaaccatt ggtaggcatc gctccgttct cgttccacga agtgagcgcg
     7441 aagatcttct ttcgtggtcc gggcatagtg cagcatttcg gcgcgcagct tgcgaaaggc
     7501 cgccaggccc gtcttgacct ccttcagttt gccggtggca tcctctccga cggccacttt
     7561 ctggcggttg gtgcggtcga taccgtacca gagaacactg tcatcggagc ctgaggggcc
     7621 gcgattcact ggtggcgcgt tcatcgcgtc ctggaagttc ctccagtaga tgggctcgct
     7681 gaccaccagg tggttgagga cctccaagat gctccaggaa cctggggcgg gacggaactg
     7741 tagttgggcc agagagaggc cggaaatctc atcgccgagc cagcttccgg tcatctctaa
     7801 gtgggcaacc aaccgctgac gctcgaggtc ggtcatcata gcggcgggcg ccagcgcggc
     7861 gcacatggcg aatacgcaga cggctacgcc aaccggcgcg ctcctcatga ctgaacagaa
     7921 taacaccgtg agatgtgacg ggatttccgg agatgttgaa attctcatac cgagcgcctg
     7981 gggagatgac ggacacgaaa tttgcgcggg gcggagtcga aggcggcagg ctgggagtat
     8041 actgaacaag acctgaagtc caaagtggaa attcccgcgg aaacagtatt cttcggctga
     8101 aggtgcgtta tgtgtgagta agtcggcagt accgaaaggc ttattcttgg attccttggc
     8161 gcatggatta tgggcagcgg cggaggctac ggtcgcaaag cggaccgccg ggctccgggt
     8221 ccgcattctg ccatttgccc tttgggccac gttccccgat atgctggcat tcgggccggg
     8281 cattgcggtc ggcctttggc tccgaattgc gggaggagca ggctatgctg aatcggccca
     8341 cggcggccac ttccaccact tccacgtcgg gttgccgttg tatgcgatgg ggcatagcct
     8401 gctcgtcttt gccagtgtct acggcctttg ctcgctggtg gcgcgccgcg gcgtcgtctc
     8461 gccactgggc tggctgctcc acatcttgat tgacattccc acgcacagct acagctacta
     8521 cgcgactcgt tttctgtggc cgctctcgaa cttcggcttc gacggagtcg cctggtggac
     8581 tccgtggttg ttgtggtgca cttatggaag cctggcgctc gtgtacttgc tgatgtggcg
     8641 aaaaggctgg ctgcgttcca cttcacgctc atcgagccgc
//
LOCUS       DATIFQ010000083         4280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2708968, whole genome shotgun sequence.
ACCESSION   DATIFQ010000083 DATIFQ010000000
VERSION     DATIFQ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2708968"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1404
                     /locus_tag="VLE22_03615"
     CDS             292..1404
                     /locus_tag="VLE22_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSB13518.1"
                     /translation="MRPDSLYERPIPERHRAVFYLGHLEAFDWNLICRGSLDIPSFQV
                     EFDRLFAFGIDPDSSGLPQDKPADWPGLEEISAYNTRVRSTLDGVLEESPQELLHVAI
                     EHRLMHAETFAYMLHNLPYDRKMGPRTAPRRNGRPVDPSMVEIPGGQAILGLQKGEAF
                     GWDNEYQRHSIDVAPFAIGKYKVTNGDYLAFVEAGATAPPFWVSRSHRWFYRGMFEEI
                     PLPLDWPVYVSYDQAAAYAAWAGKRLITEVEYQRAAFGSGDESGLSLDPARDNYDFHE
                     WDPVSVSSSRENKFGVVQLVGNGWEWTSTVFYPFPGFEPFPFYPGYSANFFDGQHYVM
                     KGASQRTAAKLVRPSFRNWFRNSYPYVYATFRVAEN"
     gene            1405..2415
                     /gene="egtD"
                     /locus_tag="VLE22_03620"
     CDS             1405..2415
                     /gene="egtD"
                     /locus_tag="VLE22_03620"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HSB13519.1"
                     /translation="MSAQPRTHSAISEFASEVHAGLAKEGQKTLPCRYFYDDVGTALF
                     QAISLLPEYGLTRADERVLRAHAGELAAWLPSPVAVLELGSGTGSKTRWVLEALSVRA
                     SVVYYPIDVSRTALQCCHRELSDVAEVVPVEASYLEGVTRAVSHRKPRERILTLFLGS
                     TIGNFERSEMVQFLQEIRLRLRPGDALLLGTDLEKAESRMTLAYDDPPGVTAAFNLNL
                     LARINRELGGDFVIREFAHQALYNRSERRIEMHLRSRRDQEVFIRDAGIAVRFRRGET
                     IWTESSHKFELSEVRTLAADSGFNCVRQWVDQEWPFAENLLIAGGKRSSNGRGFLAGS
                     AS"
     gene            2412..3764
                     /locus_tag="VLE22_03625"
     CDS             2412..3764
                     /locus_tag="VLE22_03625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSB13520.1"
                     /translation="MNASFDLVVIGTGSAAMTVAYKCRKADWRVAIVDSLPFGGTCAL
                     RGCDPKKVLVGAAEVIDFCRRMKGRGVLSGDTRIDWAGLMRFKRTFTDPVPAQREKSL
                     AEAGISTFHGHARFTAPGTIAVGQDNLSAKHFVVATGARPIPLGVPGEELLTTSDQFL
                     DLERLPSSLVFVGGGYIAMEFAHLAARAGSEVTILHRGPRPLPRFDPELVDLLVERTR
                     RLGVTIRLGTAVTAIEESGGSLKVHASASGETVTVSAEMVVHSGGRVPNVAALGLEAA
                     GIEYTRQGVTVNEYLQSVSNPAVYSAGDSAHSGGAPLTPVAGYEGRIVAANLLDGNHS
                     KPDYNGLASVAFTVPPVAAVGLSEYAAQSQGLRFRVLHEDTSGWYSSRRIAEECSGFK
                     LLVEEETGRILGAHILGDRAEEVINLFALAIRYDLTARHLSETLFSYPTHSSDVQYMV
                     "
     gene            3764..>4280
                     /locus_tag="VLE22_03630"
     CDS             3764..>4280
                     /locus_tag="VLE22_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSB13521.1"
                     /translation="MVFANMSPEPAGKAGEGPILEFRNVSYLVRNQWILREITLTVYQ
                     GETLALLGRSGAGKTTLLKMVNGLVSPTEGEVLFEGRATARWDPIRLRRRIGYVIQEV
                     GLFPHLTVEDNVGLVPRLEEWIPATRRSRVELLLNSVGLPPDQFAARYPRSLSGGQKQ
                     RVGVARALAADP"
BASE COUNT          823 a         1243 c         1345 g          869 t
ORIGIN      
        1 actgcgccgt ccagaaccgg tccaacccct acatcgtgac cgtctctgac ccaaggtcca
       61 gaaacctaag aagatgtacc ccggctgctt tcgccgctgc tgggaagacg cccatcgtta
      121 gaggtggcag tacaataaaa acaaggcaca cgcgtcactg ggacttcatt cgagacgggc
      181 agatttgctt ttatacgttt cgctatgcct accgaatcca acaactccac ttcggaactc
      241 gttgccgctc tgcagtcggc acgctcatgt acggatgccc tgttttcgcg agtgcgcccg
      301 gactcgctgt atgagcgccc tattccggag cgccatcgcg ctgtctttta cttgggacat
      361 ctggaggcat tcgattggaa cctgatctgt cgaggttccc tcgatattcc cagtttccaa
      421 gtcgagttcg acaggctgtt cgcgttcggg atcgatcccg attcgagtgg cctgccacaa
      481 gacaaacccg ctgactggcc gggtctggag gaaatcagcg catacaacac ccgcgtgcgc
      541 agcacactcg acggggttct ggaagagtcg ccacaagaac tcctgcacgt ggcgattgaa
      601 cacaggttga tgcacgccga gaccttcgcc tacatgcttc acaaccttcc gtacgaccgc
      661 aagatgggtc cgcgcaccgc acctcgcaga aacggtcggc cggtagaccc gagcatggtc
      721 gagatccctg gcggtcaagc tattctaggc ttgcaaaagg gtgaggcgtt cggctgggac
      781 aacgagtatc agcggcatag tatcgacgtc gcgccgttcg ccatcggcaa gtacaaggtt
      841 acgaacggag actacctggc ctttgttgag gcgggcgcta cggctccccc gttctgggtg
      901 tcccgctcgc accgctggtt ctatcggggg atgttcgagg agattccttt gccgctcgat
      961 tggccggtgt acgtctcata tgaccaggct gcagcgtacg cagcgtgggc ggggaagcgc
     1021 ctgatcacag aagtcgaata tcagcgtgcc gcgttcggct ccggtgacga atccgggctg
     1081 agtctcgacc ctgctcgcga taactacgat ttccacgagt gggaccccgt gtcagtcagt
     1141 tcctcgcgcg agaacaagtt cggagtggtt cagttggtag gcaatgggtg ggagtggacc
     1201 tctactgtgt tctatccctt ccccggcttc gaaccattcc ccttctatcc aggctactcg
     1261 gcgaatttct tcgatggcca acactacgtg atgaaaggcg cgtcgcagcg aacagcggcg
     1321 aagctggtca ggccctcgtt tcgcaactgg ttccggaact cgtatccgta cgtttatgcc
     1381 acgtttcgag tggcggagaa ctgaatgtcc gcgcaacccc gcacgcattc ggccatctct
     1441 gaatttgcct ccgaggttca tgccggactg gcgaaggagg gccagaagac tttgccctgc
     1501 cgttacttct atgacgacgt cgggaccgct ctcttccagg cgatcagcct gcttcccgag
     1561 tacggcctga cccgggctga cgagcgcgtc cttcgagcgc acgcagggga gcttgccgct
     1621 tggttacctt ctccggttgc cgtgctggaa ctgggcagcg ggaccggttc caagacacgt
     1681 tgggttctag aggccctttc ggtgcgcgcc agcgtcgtct actatccgat cgacgtctcc
     1741 aggacggctc tccaatgctg ccacagggaa cttagcgatg tcgccgaggt ggtgcccgtg
     1801 gaggcttcct atctggaggg cgtgacgcgg gccgtctcac atcgtaagcc acgggagcgg
     1861 atcctgacgt tgtttcttgg cagtaccatc ggcaactttg aacgctcaga aatggtgcag
     1921 ttccttcagg aaattcgctt acggttgcgc cctggcgacg cgctcctgtt gggcacagat
     1981 ctggagaagg ccgaatccag gatgacgctg gcgtacgatg atccgccggg ggtgacagcg
     2041 gcgttcaacc tgaacctcct ggcgcggatc aatcgcgagt tgggtggaga cttcgtcatc
     2101 cgggaattcg cgcaccaggc cctctacaac cgatccgaac ggcgcattga gatgcacctg
     2161 cggtcgcgtc gcgaccagga agtgttcatc agggacgccg gaatcgcggt tcgattccgg
     2221 cgcggagaga ccatctggac agagtcttcc cataagttcg agctttccga ggtcaggacc
     2281 ctggcagcag acagcggctt caactgcgtg aggcagtggg tagaccagga atggcctttc
     2341 gcggagaatc tactgatcgc cggcggtaag cgaagctcaa atggacgggg ctttctcgcg
     2401 gggagcgcgt catgaacgca agcttcgatc tggtcgtcat agggactggt tcggctgcaa
     2461 tgacggtggc atataagtgc cgtaaggcgg actggcgggt ggccatcgtg gattcgctgc
     2521 cgttcggagg cacgtgtgct ctccggggat gcgatccgaa gaaggtctta gttggggcag
     2581 ccgaggtcat tgacttctgc cgccggatga aaggcagggg cgtgctttcc ggcgacactc
     2641 gcatcgattg ggccggacta atgcggttca aacgcacctt caccgatccg gtccccgcac
     2701 agcgtgagaa gagtctggcc gaggccggca tctcaacctt tcacggacac gcgaggttca
     2761 cggcgccggg gacgatagcc gtcggtcagg acaacctctc cgccaagcat tttgtcgtcg
     2821 ctacgggagc aaggccgatc ccgttgggtg tgccgggcga ggaattgctc accaccagcg
     2881 atcaattcct ggatttagaa cgattaccct ccagcctcgt tttcgtcgga ggtggttaca
     2941 tcgccatgga gttcgcccat cttgccgcgc gagctggatc ggaggttacg atcctgcatc
     3001 gcggcccgcg gcccctcccg cggttcgacc ccgagctcgt agacttgctg gtggagcgga
     3061 cccggcgcct cggtgtcact atacggctgg gcaccgctgt cacggcgatt gaagagagcg
     3121 ggggaagcct gaaggtccac gcgtcggcaa gcggggagac agtgaccgtg agcgcggaaa
     3181 tggtcgtgca ttcggggggc cgcgtgccca acgtcgcggc cctgggtctg gaggcggcag
     3241 ggattgagta cacgcgccaa ggagtgacgg tcaacgagta ccttcagagt gtttcgaacc
     3301 cggcggtcta ttccgccgga gattccgccc acagcggagg cgcacctctc accccagtgg
     3361 ccggttatga gggaaggatt gtcgccgcga acctgctgga cggaaaccat agcaaaccgg
     3421 actacaacgg actggcaagt gtggccttca ccgttccgcc ggtggccgcg gttggcctct
     3481 ccgagtatgc tgcacagagt caagggctgc gcttcagggt gcttcacgag gacacctcgg
     3541 gttggtactc ttcccgccgg atcgcggagg aatgctcggg cttcaagtta ctggttgagg
     3601 aggaaaccgg gcgcattctc ggcgctcaca ttctggggga ccgggccgaa gaagtcatta
     3661 acctgttcgc cttggcaatc cgctacgatc tgacggcgcg ccatctgagc gagacactgt
     3721 tttcgtatcc cacccacagc tcggacgttc agtacatggt ctaatggtct tcgcgaatat
     3781 gagccccgaa cccgctggga aggctggcga aggccccata ctggaattcc gcaatgtgtc
     3841 gtatctcgtg cggaaccagt ggattctgcg cgaaatcaca ttgacggtat accagggcga
     3901 aaccttggcg ctgttgggac ggagcggtgc cggaaaaacc acgcttctca agatggtcaa
     3961 cggacttgta agcccgacag aaggtgaggt gctctttgag ggcagggcga ctgcacgctg
     4021 ggatcctatc cggctaagaa ggcggatcgg ctatgtgata caggaggtgg gcctcttccc
     4081 gcacctgaca gtggaggata acgtagggct ggtgccccgg ctggaggaat ggattcctgc
     4141 gacaagaaga tcccgcgttg aactgctgct gaactcggtg ggacttccgc cggatcagtt
     4201 cgcggcacgc tacccccgga gtttgtcggg ggggcagaag cagcgagtgg gggtggcccg
     4261 ggcccttgca gccgacccac
//
LOCUS       DATIFQ010000084         5063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1559056, whole genome shotgun sequence.
ACCESSION   DATIFQ010000084 DATIFQ010000000
VERSION     DATIFQ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559056"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VLE22_03635"
     CDS             complement(<1..471)
                     /locus_tag="VLE22_03635"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSB13522.1"
                     /translation="MTEQDKMSRPLVEVDPEVFAAIRSELERQNSGLELIASENFTSE
                     AVLEATASVFTNKYAEGYPGRRYYGGCEFVDVVENLARDRAKKLFQCEHANVQPHSGS
                     QANQAAYAAVLQPGDTILGMNLAHGGHLTHGHHLNFSGKTYKVVPYGVRREDEII"
     gene            complement(468..944)
                     /gene="rpiB"
                     /locus_tag="VLE22_03640"
     CDS             complement(468..944)
                     /gene="rpiB"
                     /locus_tag="VLE22_03640"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HSB13523.1"
                     /translation="MKIAIGADHAGFLLKERLRDKLLRDGHHVVDYGTHSTESTDYPD
                     YAAPVARDVASGEADRGVLICATGIGMSIAANKVPGVRAALGTSEEEVRLVRGHNDAN
                     ILTLGGKFIEPAQAEKLLDVFLSAQFEGGRHARRVAKIGRMERGGAGMDESRGEKA"
     gene            1013..2731
                     /locus_tag="VLE22_03645"
     CDS             1013..2731
                     /locus_tag="VLE22_03645"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HSB13524.1"
                     /translation="MHWSKLFIPTLRENPAEAEVPSHQLLLRAGYIRQLSAGIYSYLF
                     LAQRSLLKVTRIVREEMDSIGAQEMLLPALHPAELWQESGRWEVMGDTMFRLKDRFQR
                     DLCLGMTHEEVMTVIARGELRSYKQLPQIWYQIQTKFRDEPRPKSGLLRVRQFIMKDS
                     YSFDIDASGLDAAYDKHYRAYCRIFSRCGLQYLVVEAHSGAMGGSQSHEFMVAAEAGE
                     DWVAVCPSCKYAANLEKAEAVPSPPAAADPEGDLNPEKFHTPGRKTIAEVAEFTSLPE
                     TSQIKSLVMVADGKPFLALLRGDHSLSETKFAGVAKAAEIRPAHPEEIRQWFGAEAGS
                     LGPVGAKIPIVADLALKGRRNMIAGANEDDYHLRHVTPDEDFRPEWRDLRQVAAGDAC
                     TQCGGSLELKKTVEIGHIFKLGYKYSDSMGLRVLNAAGEEVTPIMGSYGIGIERILSA
                     AIELYNDKDGMALPVAIAPFTVVVTPVNFADEAQRKAAEQIYSSCLSLGLDALLDDRD
                     ERPGVKFKDADLIGIPFRVTVGKKLAQGIVEVVERRSRKSADVPVGEVVEFVAGKIKA
                     ALENGC"
     gene            2721..3851
                     /locus_tag="VLE22_03650"
     CDS             2721..3851
                     /locus_tag="VLE22_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB13525.1"
                     /translation="MAVDWKAIRSEFPALERWTYLNTATYGQLPRRSVEAAARHFSHR
                     DELACADFLDWFDDADRIRGLIARLIHCEAADIAFIPSAAHALSLLLGGLEWRAGDRV
                     VTLADEFPNNLYNPALLRRHGVEAVEASWPEFYNAITPRTRLVVLSEVNYTSGFRPPL
                     AEIAAFLRERGVLLYIDGTQSVGALEFDMAAIRPDMLAVHAYKWLLSPNGAGFLYVAP
                     GLRERLEPNVIGWRSHRDWRRVDNLHHGAPEFKSEAERYEGGMLAFPVIYAMGASVGM
                     MLEIGPEVIARRVRELTDTTCAVLRRLGARLLRDESPRLYDSQIIAARFEGADASALA
                     RKLREGGVLISARHGNLRISSHFYNNEDDIARLETELRKTRD"
     gene            complement(4213..4641)
                     /locus_tag="VLE22_03655"
     CDS             complement(4213..4641)
                     /locus_tag="VLE22_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HSB13526.1"
                     /translation="MTADEGAIQDILKQFEAAWNRYDSVGIGALFAEDANFIHIFGGQ
                     LDGRTAIEAAHRVIFNTIYRGSHASFPLRSIRFLRPDVAVVFARAHVQFKEGDEAREI
                     ETRPTLVVVKEQAKWQVVAFQNTKISEVPTAAQAAARLAT"
BASE COUNT          873 a         1674 c         1525 g          991 t
ORIGIN      
        1 gatgatctcg tcttcgcgcc gcacgccgta cgggaccacc ttgtaggtct tgccggagaa
       61 attcaggtgg tgcccgtgcg tcaggtgccc gccgtgcgcc aggttcatgc cgaggatcgt
      121 gtctcccggc tggagcaccg ctgcatacgc ggcttggttt gcctgcgagc cggagtgcgg
      181 ctgcacgttg gcatgttcgc actggaagag cttcttcgcc cggtcccgcg ccaggttctc
      241 gaccacgtcc acgaactcgc agccgccgta ataccgccgc ccgggatacc cctcggcgta
      301 cttgttcgtg aacacgctcg cggtggcttc cagcaccgcc tccgacgtga agttctcgct
      361 cgcaatcaac tcgagcccgg agttctgccg ctcgagctcg ctccggatcg cggcgaacac
      421 ttcgggatcc acttcgacca gcggcctcga catcttgtct tgttccgtca tgctttttcg
      481 ccccgtgatt cgtccattcc ggccccgccg cgctccatcc ggccgatctt cgcgacccgg
      541 cgagcgtgac gcccgccttc aaactgcgcg ctgagaaata cgtcgagcag cttctccgct
      601 tgagccggct cgatgaactt tcccccgagc gtcaggatgt tggcgtcgtt gtgaccccgg
      661 accagccgca cttcctcctc ggaagtaccc agggcggccc gcacgcctgg taccttgttc
      721 gcggcgattg acatcccgat gccggtcgcg cagatcagca cgccgcggtc ggcctcgccc
      781 gaggcaacgt cccgcgccac cggcgcggcg tagtccgggt agtcggtgga ctccgtgctg
      841 tgggtgccgt agtccaccac gtggtgcccg tcgcggagca gcttatcccg aagacgctcc
      901 ttcaacagaa agcccgcgtg gtcggctccg attgcgattt tcatggggga ttctgctcca
      961 ttttagcatg ctagaatgag tgcttcgtct cttttccccg tcgagtaact tcatgcattg
     1021 gagcaaattg ttcattccca ccctgcggga aaatcccgcg gaggcggagg ttccgagcca
     1081 tcagcttctc ctgcgcgccg gctacatccg ccagttgtcg gccggcattt acagctactt
     1141 gttcctcgcc cagcggtctc tcctgaaggt cacccggatc gtccgtgagg agatggattc
     1201 catcggggcg caggaaatgt tgctgcccgc tctccacccc gccgaactct ggcaggagtc
     1261 cggacgctgg gaggtcatgg gcgacaccat gttccgcctc aaagaccgct tccagcgtga
     1321 cctctgcctc ggcatgaccc acgaagaggt catgaccgtc atcgcccgtg gtgagctgcg
     1381 cagctacaag cagcttccgc agatctggta ccagatccag accaagttcc gcgacgagcc
     1441 gcggccgaaa tccggcctgc tccgcgtccg gcagttcatc atgaaggact cctacagctt
     1501 cgacatcgac gcctccggtc tcgacgccgc ctacgacaag cactaccgcg cctactgccg
     1561 gatcttcagc cgttgcgggc tgcaatacct cgtagtcgag gcgcactccg gcgccatggg
     1621 cggcagccag tcgcacgagt tcatggtggc ggcggaagca ggcgaggact gggtagccgt
     1681 gtgcccgtcc tgcaagtacg cagcgaactt ggagaaggcg gaggcggttc catcacctcc
     1741 ggcggcggcc gatccggagg gcgatctcaa ccccgaaaag ttccacaccc ccggccgcaa
     1801 gaccatcgcc gaggtggccg aattcacctc gctgccggag acatcgcaga tcaagagcct
     1861 cgtcatggtg gcggacggga aaccttttct cgccctgctg cgcggcgacc actcgctgag
     1921 tgaaacgaag ttcgccggag tcgcgaaggc ggctgaaatt cgtcccgcgc atcccgagga
     1981 gatccgccag tggtttggcg ccgaagccgg ctcgctcggc cccgtcggcg cgaagatccc
     2041 catagtcgcg gatctcgcct tgaaaggccg ccggaacatg atcgccggcg ccaatgaaga
     2101 cgactaccat ctgcgccacg tcaccccgga tgaggatttc cggcccgagt ggcgcgacct
     2161 gcgccaggtc gctgccggcg acgcctgcac acagtgcggc ggttctctgg agctgaagaa
     2221 aaccgtcgag atcggccaca tcttcaagct cggctacaag tactccgact cgatgggcct
     2281 ccgcgtcctg aacgccgccg gtgaagaggt cacgccgatc atgggttcct acggcatcgg
     2341 catcgagcgg atcctgagcg ccgctatcga gctgtacaac gacaaggacg gaatggctct
     2401 gcccgtcgcc atcgccccgt tcaccgtcgt cgtgacgccg gtgaacttcg ccgacgaagc
     2461 acagcgcaag gcggccgaac agatttactc ttcctgcctt tccctcggcc tcgatgcgct
     2521 gctcgacgac cgcgacgagc gtccgggagt caagttcaag gacgccgacc tgatcggcat
     2581 tcccttccgg gtgacggttg ggaagaaact cgcccagggc atcgtggaag tcgtcgagag
     2641 gcgcagccgg aagtcggccg acgtccccgt gggcgaggtg gtcgagttcg tggcgggtaa
     2701 gatcaaggcc gctctcgaaa atggctgttg actggaaggc aattcgatcc gagttccccg
     2761 ctcttgagcg gtggacgtat ctgaacacgg ccacgtacgg ccagcttcct cgccgctctg
     2821 tcgaggcggc cgcccggcac ttttcgcacc gcgatgagct ggcctgcgcg gacttcctgg
     2881 actggttcga cgatgccgac cgcattcgcg gcctcattgc ccgcctcatt cactgcgagg
     2941 ctgccgacat cgccttcatc cccagcgcgg cgcatgcgct gtcccttttg ctcggcgggc
     3001 tggagtggcg ggccggggac cgcgtggtca cgctcgccga cgagtttccg aacaacctct
     3061 acaatcccgc gctgctgcgg cggcacggcg tcgaggctgt cgaggcgtcc tggccggaat
     3121 tctacaacgc gatcaccccg cgcacccgtc tcgtcgtcct gagcgaggtc aactacacga
     3181 gcggcttccg gccgcccctt gcggagatcg cggcgttcct gcgggagcgc ggcgtgctgc
     3241 tgtatatcga tggcacgcag agcgtcggcg cgcttgagtt cgacatggcc gccatccggc
     3301 ccgacatgct ggcggtccac gcttataagt ggctgctctc gccgaatggc gccggcttct
     3361 tgtatgtcgc gcccggcctg cgggagcgcc tggaacccaa tgtgatcggc tggcgcagcc
     3421 accgcgactg gcggcgcgtg gacaaccttc atcacggcgc gccggaattc aagagcgagg
     3481 ccgagcggta cgaaggcggc atgctggcgt ttcccgtcat ctatgccatg ggcgcttccg
     3541 tcggcatgat gctcgagatc ggccccgagg tgatcgcgcg ccgcgtgcgc gagttgacgg
     3601 acacgacctg cgcggtgctg cgtcgtctcg gcgcccggct cctgcgcgac gaatcgccgc
     3661 ggctctatga ttcgcagatc atcgccgccc gcttcgaggg cgccgacgca tccgcgctgg
     3721 cgcgcaagct ccgcgagggc ggcgtgctga tctccgcccg ccacggcaac ctccgcatct
     3781 cctcgcactt ctacaacaac gaagacgaca tcgcccgcct ggaaaccgag cttcgcaaaa
     3841 cccgggactg actccaaatt tcgcagcgtt tgcgaaattg agcgggacgg gcctactctg
     3901 tcctcagcgt tgttcgcgag cggacagagc tgcctgtccc gtctgtctcg gagtttcggg
     3961 gattcttagc cgggctacca tttccccgcc gttgcgagca aaacccggga ctgactccga
     4021 atttcgctgt atctgcgaaa ttggtgtctg tcccgggttt tgcccttcgc gcccagagtc
     4081 accgcggaag aaatgggaca gaccaccatt tcgccgttgt tgcgaaatgg ctgtctgtcc
     4141 caatttcttc cagcctcggc cacagtgagc ggaagctcca gcgaagtttc tcagcacccg
     4201 gaggtttcca tactaagttg caagacgggc cgcagcctgg gctgccgttg gcacctcgga
     4261 aatcttggta ttttggaagg ccacgacttg ccactttgcc tgttccttta ccacgaccaa
     4321 ggttgggcgg gtctctattt cgcgcgcttc atcgccttcc ttgaactgca cgtgcgctcg
     4381 ggcgaagacg accgcaacgt cggggcgcaa aaagcgaatg ctccgtaatg ggaaactggc
     4441 gtggctgcct ctgtagatcg tgttgaagat gacccggtgc gcggcttcga tggccgttcg
     4501 cccgtccaac tgccccccaa aaatgtggat gaagttggca tcctccgcaa acaacgcccc
     4561 gatgcccaca ctgtcgtaac gattccacgc ggcttcaaat tgcttcagga tgtcttgaat
     4621 ggcgccttca tcggctgtca tgactatccc cccttggatc agaatactat caactccagt
     4681 ttcggtgaca agctacgcga tcgcggtggt gcgggcggcg ggccgtgaat ttggggattc
     4741 gtagcctttc cgggactgcc tccaaatttc gctatgttta gcggaagaaa tgggacagac
     4801 caccatttcg ccgcatgcag gtaaccgagc cgcgaccgaa agggagcgga cgcgcgcgct
     4861 tccggatctt ggcccgtatt cgcatgcagc taccacggca gaaatgggac aggccaccat
     4921 ttcgccgttg ttgcgaaatg gctgtctgtt ccaataggcg gtaacttacc ccttgagtgt
     4981 tgcgcagagc ctttcgagcg tccgcgccgg ttcggggtcc gactcaccag tcctatgaca
     5041 agccgacgat atatacttat gaa
//
LOCUS       DATIFQ010000085         8711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2709151, whole genome shotgun sequence.
ACCESSION   DATIFQ010000085 DATIFQ010000000
VERSION     DATIFQ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2709151"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VLE22_03660"
     CDS             <1..683
                     /locus_tag="VLE22_03660"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB13527.1"
                     /translation="RGGNTCRGIVRSVMRDTVMRPALILMLASAFCVAAAGEWQSLFD
                     GKTLEGWRETPFTGRGQVRVENGTILLGRGKPMTGVTWAGSLPKSGYEVRFEGARLEG
                     NDFFASLTFPVEDSFCTWVTGGWGGDIVGLSSLDGWDASDNETRTYFNFENGRWYALR
                     LQVTADRIMAWIDERRVINVEIRGRSIGLRYGETKLSVPFGFASYATAGALRKIEYRL
                     LPESGAAR"
     gene            complement(1113..2723)
                     /locus_tag="VLE22_03665"
     CDS             complement(1113..2723)
                     /locus_tag="VLE22_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB13528.1"
                     /translation="MQRRDLLKSAAASAGLTILKSGTLRGQNAPSNRLNIALIGVWGR
                     GTAHYNTMRNENVVALCDINDLRLKEAVQIFPKAKTYWDWRRLLDQKDVEAVIICTAD
                     HHHAFIANWALNRNMHVFCEKPLGISVEEVRTVRANYLKRKVKLATQHGTQRHAYPNF
                     ERVRELILDGAIGELKTIHSWDSRKLPRPGYPAGEGMPPSFLHYEQWIGPSPYHPYSP
                     QYFGGSNGLNCLFWNMYRDFGVGQMGDMGAHTMDLVWNAIDAGAPTAVEVDQEVTDKY
                     DPNITPVRLKASFEHPANAWRGPVTVVWYQGGLKPEAPKGYIDVESIGNGAIFEGTKG
                     SILADFTTRVIIPNNDDGDLTYYKRRTKEQLLPLIRGTGQVTQAPPRPARRPPAAQRA
                     AAPPLPRGMTAMPSAEVGPSGFPTIQFLEGGIPPALGLPNPAVDAILAAEKAGRTPGG
                     QADVFQLEWIDACKGKNDRVVNGTSSKTHCDFDYSGTMMEQMLLGLVAHRVGKRLEYD
                     GATGRVTNAQEANDYLKREYRANWTLNG"
     gene            2806..3564
                     /locus_tag="VLE22_03670"
     CDS             2806..3564
                     /locus_tag="VLE22_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HSB13529.1"
                     /translation="MTRREFVAAGAAGAATVAEAGAQAGRPAPRILAVSAHPADFCSR
                     AGGTLIKHVRAGSKAKVIWLTQGESDESDFLIKQRPGISVAEVRRVREKEAFACAEII
                     GAEGKMFGFGDGPLLMTPERMEMLVREIADFKPDLILTHWKDELTYPTHWRTSRSVIE
                     AAQMARVSWDIRFFEPNIGTASRVGFVPDHYVDISEVFERKVEALKTLAAQPRLVSDY
                     TACNRWRGIECGRQYAEAFVRWAPKPPVYELLGA"
     gene            complement(3644..4255)
                     /locus_tag="VLE22_03675"
     CDS             complement(3644..4255)
                     /locus_tag="VLE22_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13530.1"
                     /translation="MIQTFMKSWWLLALCGVLDAIISVVNLLMQDPDGALTLRTFAVK
                     STAVFLGKVALAAGVCTVAAGIWNSRKGKSWLLVLNGLALSAFGLLCFWTDRRLDFRT
                     FALLLVVMAVSFGIFALATALTLRHQVPDKWFLSLAGAASVGFALAFLALGFRWIELV
                     QPESLFLWLSSYFAFSAICMLGLGLISLRGATHRMAGSALPTG"
     gene            complement(4380..4670)
                     /locus_tag="VLE22_03680"
     CDS             complement(4380..4670)
                     /locus_tag="VLE22_03680"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSB13531.1"
                     /translation="MRFLSIVLILSVVAFSCPALFAQTGSSDDRIYDEVRRKLAADSV
                     VKGGGFEVDVKDGVVTLRGKVREEKQKRRAERITKKVKGVKSVVNELRVEPY"
     gene            complement(4672..5598)
                     /gene="era"
                     /locus_tag="VLE22_03685"
     CDS             complement(4672..5598)
                     /gene="era"
                     /locus_tag="VLE22_03685"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HSB13532.1"
                     /translation="MNEAAPAGFVSGFVSILGRPNSGKSTLLNTLVQMKVAIVAEKPQ
                     TTRTSIQGVLNLPGAQIIFVDTPGIHEPDSAFNQRMLETVRAALAERDLLLYLADATR
                     PFTDADRKAVELVKQARTPALLLLNKIDRIHDKGPLLTLIEQYKAAHEFEEYIPISAL
                     TGDGLDVVRDCIVKRLPPGPPYFPPDHVTDQPERFMVAEIVREKILHETRQEVPHAVA
                     VLIDYWEEKERVTRIAATIYVERVGQKAIVIGAKGAMLKRIGTLAREEMENLLGRKVF
                     LELHVKVRPKWREDPRFLDALDWRSMAGTGVE"
     gene            complement(5591..6850)
                     /locus_tag="VLE22_03690"
     CDS             complement(5591..6850)
                     /locus_tag="VLE22_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSB13533.1"
                     /translation="MLILLVVLAFWFGATLVVVTYTQMLYLESLRLRTRDLPAFTFFR
                     EKLEERIGLKSERGALAFSLIKHTTLALLGVVVLAIASTRSAMLWEAVIEASIASWLV
                     MFLGAYALPHALYRRTEGRWLLPLVPVLRALAVAVRPLTAMLEFFQSVVDLGEPGDAE
                     EEPNHVENLDALISAGAEEGLIEEEDRKLIQAAVAFGDKTVREVMTPRPNIIAIEAKK
                     TLEDLRDLVINEQYSRVPVYEESIDHIVGFVHVRDMFELDEEERIGRAVGDLMRPVRF
                     VPETKLVSELMREMQEDRSHMAVVVDEYGNTAGLATMEDLVEEILGEIRDEHEPELDV
                     TAEPDGAYVVSGSFDLDRLHELLQFRPGGETESTTIGGLAAEWLGRVPSAGEIVERDG
                     LRIEVLSGNERRVDLVRIRRSEGEENE"
     gene            complement(6844..7293)
                     /gene="ybeY"
                     /locus_tag="VLE22_03695"
     CDS             complement(6844..7293)
                     /gene="ybeY"
                     /locus_tag="VLE22_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HSB13534.1"
                     /translation="MPTFEDPLIFLRVRSGLSRRRLRELAKHLAATVGGGRRFTCLLT
                     DDRELRRLNRQFLGKDYPTDVLSFPEPGPDDFLGEMAISVERAAAQAREYGHAVEDEI
                     GILLLHGLLHLTGMDHETDRGRMARAEKRWRKALGLPAALVERARRC"
     gene            complement(7303..8334)
                     /locus_tag="VLE22_03700"
     CDS             complement(7303..8334)
                     /locus_tag="VLE22_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HSB13535.1"
                     /translation="MKVSVEISRGIESLFGTRDENIRLLETGLNVSTQLLEDCLEIEG
                     DEANVARAERILEDYVSLVREGHTFANGDLNSYLRVVTEDPGVRLRDLVLSGRQRSFG
                     KKVVAPKSGNQRRYVDAIERHDLVFGIGPAGTGKTYLAVAMAVSALMAKQVSRIILTR
                     PAVEAGERLGFLPGTLQEKVDPYLRPLYDALYDMLDVDKVEKFLERSVIEIAPLAFMR
                     GRTLNDSFIIMDEAQNCTPEQMKMILTRQGFNSKMVVTGDVTQIDLPIAKRSGLLEAI
                     EVLRGVEGIRFVNFDERDVVRHNLVQRIVKAYERYGETNGSSGQLKLKLGDPAGERPV
                     DGEPARDGN"
BASE COUNT         1672 a         2586 c         2809 g         1644 t
ORIGIN      
        1 ggagaggcgg caacacatgc cgtggtatag tccgtagcgt catgcgagac acggtgatga
       61 ggccggcgtt gattctcatg cttgcgtcgg cgttttgcgt tgccgcggcc ggggaatggc
      121 aatccctctt tgacggcaaa acgctggagg ggtggcggga gacgccgttc acgggacgcg
      181 gccaggtccg ggtggagaat ggaaccatcc tgctcggacg cggcaagccc atgaccggcg
      241 tcacgtgggc cggttcgtta ccgaagtccg gctatgaggt ccgtttcgaa ggagcacggc
      301 tcgaaggcaa cgatttcttc gccagcctca catttcccgt cgaagattcg ttttgcacct
      361 gggtgaccgg cggatggggc ggcgatatcg tcggactctc cagcctggac ggatgggatg
      421 catcggacaa cgaaacgcga acctacttca actttgagaa cggccgttgg tatgcgctgc
      481 gcctacaagt gaccgcggac cggatcatgg cctggatcga cgagcggcgg gtcatcaacg
      541 tcgagatccg aggccgatca atcggtcttc gatacggtga gaccaaacta tccgtgccct
      601 tcggctttgc ctcgtacgca acggcgggag cgttgcggaa aatcgaatat cgcctgttgc
      661 cggagtccgg ggcagcgcgt tgaaaaaacc tgaactgggg gcatttgtcc tcatggccaa
      721 ccacgtgcac gttctgctgc tgccccagat tgcccccagc cagttgctga aatcgctcaa
      781 agggtacacg gcccgggagg cgaaccgtct gttgggcaga acggggggca cccttctggc
      841 aacgcgaatc gtacgatcat tgggtgcgcg atccagcgga gtggacacgg attgccgcgt
      901 atatcgagga caacccagtg aaagccggac tggtctcgcg ggccgaagaa tatcgctggt
      961 ccagtgcgca cgagagatgg cgactgcggc tcagcgccac aggagccgac aggggtgtcg
     1021 gctcggcacg caccagtgcg tgcgccacga tcgagaatcc ggacgcctta agttagcgca
     1081 tgtgggtctg gagacctacc ctacgtcaag cgctagccgt tcagcgtcca gttggcccgg
     1141 tactcacgct tgagatagtc gttggcttcc tgcgcattgg tcacccgtcc ggtggcgccg
     1201 tcgtactcga gccgtttgcc gacgcggtgc gccaccagac cgaggagcat ctgctccatc
     1261 atcgtgccgg agtagtcaaa gtcgcagtgc gtcttgctgc tggtgccgtt tacaaccctg
     1321 tcgttctttc ctttgcaggc gtcgatccac tccaactgga aaacgtctgc ctggccaccc
     1381 ggggtccggc ctgccttctc ggcggcgaga atggcgtcga cagcggggtt aggaagcccg
     1441 agagccggtg gaattccgcc ctccagaaac tggatcgttg ggaaaccact cggaccgact
     1501 tcggcgctcg gcattgccgt catgccgcgc ggcagcgggg gagcagcggc gcgctgtgcg
     1561 gccggggggc gccgggcggg ccgcggaggc gcctgcgtca cctgcccggt gccgcggatc
     1621 agcggcaaaa gctgttcctt cgtgcggcgc ttgtaatacg tcagatcgcc atcgtcgttg
     1681 ttggggatga ttacccgggt ggtgaaatcg gcgagaatgc tgcccttcgt gccctcgaag
     1741 atcgctccgt tcccgatgct ctccacgtcg atgtagccct tgggcgcctc gggcttcaga
     1801 ccgccctgat accagaccac ggtgaccggc ccgcgccagg cgttggcggg gtgttcgaag
     1861 ctggccttca accgaacggg ggtgatgttg ggatcgtatt tgtcggtgac ttcctggtcg
     1921 acctcaacag ccgttggggc gccggcgtcg atcgcgttcc agaccagatc catcgtgtgg
     1981 gcgcccatat cgcccatctg gccgacgccg aaatcccggt acatattcca gaacaagcaa
     2041 ttcaggccgt tcgaaccgcc gaaatactgc gggctgtaag gatggtacgg cgacggtccg
     2101 atccattgct cgtagtggag gaacgacggc ggcatgccct cgcccgccgg gtaccctggc
     2161 cgcggcagtt ttcggctgtc ccagctatgg atggtcttca gctcgccgat agcgccgtcg
     2221 agaatcaact cgcgcacgcg ttcgaaattc gggtacgcgt gccgctgggt gccatgctgt
     2281 gttgccagct tgaccttccg cttcaggtag tttgcgcgga ccgtgcgcac ctcttcgacg
     2341 ctgatgccga ggggcttctc gcagaagaca tgcatgttgc gattcaaggc ccagttggca
     2401 atgaaggcgt ggtgatgatc ggcggtgcag ataattacag cctcaacgtc cttctgatcg
     2461 agaagcctgc gccagtccca gtatgttttg gccttcggga agatctggac ggcttccttg
     2521 aggcggaggt cgttgatgtc gcacagggcc acgacattct cgttcctcat cgtgttgtag
     2581 tgcgcggttc cgcggcccca cacgccgatg agtgcgatgt tcagcctgtt gctgggggcg
     2641 ttctggcctc tcagggtgcc gctcttcaga atcgtcaatc ccgcggaggc ggccgcggac
     2701 ttcagcagat cacgtcgttg cattgcacag tcctcctgaa catactatcg gagcttgaca
     2761 gaagatgggg agacgtgagt tgatgtgcgg cgggagggaa atggaatgac gcgtcgcgag
     2821 tttgtggccg ccggggcagc cggggctgca acagtggcag aggcgggcgc ccaggcggga
     2881 cggccggcgc cgaggattct ggcagtcagc gctcacccgg ccgatttctg ttcgagggca
     2941 ggcggcacgc tgatcaagca cgtgcgtgcg ggatcgaagg cgaaagtgat ctggctcaca
     3001 caaggcgagt ccgacgaaag cgactttttg atcaaacagc ggcccggcat ctccgtcgcg
     3061 gaagtgcgcc gcgttcgcga aaaagaggcg tttgcgtgcg cggaaatcat cggcgccgag
     3121 ggaaaaatgt tcgggttcgg cgacggcccg ctgctcatga ctcctgagcg aatggagatg
     3181 ctggtgcggg aaatcgcgga cttcaagccc gacctgattc tcacgcactg gaaggatgaa
     3241 ctcacctacc cgacccactg gcgaacgtcg cgcagcgtga ttgaagcggc gcagatggcg
     3301 cgggtgtcct gggacatccg gttcttcgag ccgaatatcg gcaccgcgtc gcgggtggga
     3361 ttcgtgccgg atcactacgt ggatatcagc gaggtcttcg agcgcaaggt ggaggcattg
     3421 aagacgctcg ccgcacagcc ccggctcgtt tccgactaca cggcctgcaa ccgctggcgc
     3481 ggtatcgagt gcggccggca gtacgccgag gcgttcgtgc gctgggcgcc gaagccgccg
     3541 gtgtacgagt tgctgggtgc gtaaaggcaa aaccctggga cagacaccaa tttcgcaaac
     3601 gcatcgaaat ttggagtcag tcccggattc tgcacaaccg aactcagccg gtgggcaatg
     3661 cgctgccggc catgcgatgg gtggcccccc gcagactaat caggcccagc cccaacatgc
     3721 agattgcgct gaaagcaaag taagagctca gccaaagaaa gagtgattca ggttgtacca
     3781 gttcgatcca gcgaaacccc aaagcaagaa atgccaaagc aaaaccaacc gaggccgctc
     3841 cagccaggct aagaaaccac ttgtccggaa cctgatggcg cagggttagc gcggtcgcca
     3901 gtgcaaaaat accgaagctc actgccatca cgacgagcag gagcgcgaac gtgcgaaagt
     3961 ccagtcttct gtctgtccaa aagcagagca gtccgaatgc gctaagagca aggccattca
     4021 gcaccagaag ccaggatttg ccctttctgg aattccaaat gcccgctgcg accgtgcaaa
     4081 ctcctgccgc cagtgcgact ttacccagaa acacggccgt gctcttcacc gcgaatgtac
     4141 gcaaagtcag ggcgccatca gggtcctgca tgagaaggtt cacgaccgat atgatcgcgt
     4201 caagaacgcc gcacaatgcc agtaaccacc agctcttcat gaacgtttgg atcatgcgca
     4261 cccccagatc gtgatccagc actacttggg cgggaggcct ctgtcctaat catagtactg
     4321 cgacgcggga tctcccggcg gaacgagaaa cgccgtgtcc ccgtttttcc cgcggagaat
     4381 cagtagggct cgacgcggag ttcgttgacg acgctcttga cgcccttgac cttcttggtg
     4441 atacgttcgg ctctgcgctt ttgcttctcc tcgcggacct taccgcggag ggtgacgacg
     4501 ccgtccttaa cgtcgacttc gaagccgccg cctttcacga cggagtcggc ggccagcttg
     4561 cggcggacct cgtcgtagat acggtcgtcg gaagaacccg tctgcgcaaa caacgccggg
     4621 caggagaacg ctacaacgct cagaataaga acaatcgaaa ggaatctcat ttcattccac
     4681 cccggtaccg gccatagaac gccagtctaa cgcatccagg aagcgcgggt cctcacgcca
     4741 cttgggccgc actttgacgt gcagttccag aaagactttc cggccgagga gattttccat
     4801 ctcctcgcgt gccagagtgc cgattctttt cagcatggcg cccttggcgc cgatgacgat
     4861 cgccttctgc cccacccgct ctacgtagat cgtggcggcg atgcgtgtga cgcgttcctt
     4921 ctcttcccag tagtcgatca ggacggcgac ggcgtgcggc acttcctgcc gcgtctcgtg
     4981 gaggatcttt tcacggacga tctcagcgac catgaaacgc tctggctggt ccgttacgtg
     5041 gtccggtgga aaatagggcg ggccaggcgg aaggcgcttc acgatgcagt cgcggacaac
     5101 gtccaggccg tccccggtga gtgccgaaat tgggatgtat tcctcgaact cgtgcgcggc
     5161 cttgtactgc tcaatcaggg tgagcagcgg acccttgtcg tggatgcgat cgatcttgtt
     5221 caacagaagc agcgccgggg tgcgggcctg cttcacaagt tccacggcct tgcggtccgc
     5281 gtcggtgaac gggcgcgtgg cgtcggcaag gtagagcagc aggtcgcgct cggcgagagc
     5341 ggcgcgcacg gtttcgagca tacgctggtt gaacgccgaa tccggttcgt gaatgcctgg
     5401 agtatcgaca aagataatct gcgcgccagg aaggttcagc acgccctgga tggaggtgcg
     5461 cgtggtctgc ggcttctcgg cgacgatcgc gaccttcatc tggacgagcg tattcaacag
     5521 cgtcgacttg ccggagttgg gacgccccag gatggaaacg aagccggaga caaagccggc
     5581 gggcgcagct tcattcattc tcttcgccct ccgagcgccg gatgcggacc aggtcgacgc
     5641 ggcgttcatt accggacaag acttcgatac gaaggccgtc gcgctcgacg atttcaccgg
     5701 cggaggggac gcgcccgagc cattctgccg cgaggccgcc aatggtggtg gattcagtct
     5761 ctccgccggg gcggaactgg agcagctcat ggagacggtc cagatcgaag ctgccggaga
     5821 caacataggc cccgtcaggc tccgcggtca cgtcgagctc cggttcgtgc tcgtcgcgga
     5881 tttcaccgag gatctcctcg acgaggtcct ccatggtcgc cagaccggca gtgttgccat
     5941 actcgtcaac cacgacagcc atgtgcgagc ggtcttcctg catctcacgc ataagttcgc
     6001 tgacaagctt ggtttcgggc acgaagcgca ctggccgcat aagatcgcca accgcgcggc
     6061 cgatcctctc ctcctcatcc agttcgaaca tgtcgcgcac atggacgaag ccgacgatat
     6121 ggtcgatgga ctcctcatag acgggtacgc gcgagtactg ttcgttgatg acaaggtcgc
     6181 gaaggtcttc gagggtcttc ttcgcctcga tggcgatgat gttcggccgc ggagtcatga
     6241 cctcgcgcac ggtcttgtcg ccgaaggcga cggcggcctg aatgagcttg cggtcttcct
     6301 cctcgatcag tccctcctca gcgccggcgg agatgagtgc gtcgaggttc tcaacgtgat
     6361 tcggttcctc ctcggcgtca cccggctcac ccagatccac gacggactgg aagaactcaa
     6421 gcatcgccgt gagcggccga accgcgaccg cgagcgcgcg gagcacgggg acgagcggca
     6481 acaaccagcg gccttcggtg cgccgataga gcgcatgggg cagcgcatac gccccgagga
     6541 acatcacgag ccaggacgcg atcgacgctt cgatgacagc ctcccagagc atggcgctgc
     6601 gggtggacgc gatcgccagg accacgacgc cgaggagggc cagggtcgtg tgcttgatca
     6661 acgaaaaggc aagcgccccg cgctcggact tgagcccgat gcgctcttcc agcttctccc
     6721 tgaaaaaggt gaacgcgggc aagtcgcgcg tgcgcagccg cagcgattcc agatacagca
     6781 tctgggtgta ggtgacgact accagagtcg cgccgaacca aaacgccaga acgacgagga
     6841 ggattagcat cggcgggcgc gctccaccag agcggcggga aggccgagcg cctttcgcca
     6901 gcgcttttcc gcccgagcca ttcggccgcg gtcggtttca tgatccatcc cggtgaggtg
     6961 cagcaggccg tgcagcaaca ggatgccgat ttcgtcctcg actgcgtgac cgtactcacg
     7021 cgcctgggcg gcggcacgct cgacggagat ggccatttca ccgaggaagt cgtctgggcc
     7081 gggctcggga aaggaaagga cgtcggttgg gtagtccttg ccgaggaact gccggttgag
     7141 acggcgcaac tcgcggtcat ccgtaagcag gcacgtgaag cgccggccac caccgacggt
     7201 ggcggcgaga tgtttggcga gctcgcgaag acgccggcgc gaaagcccgc tgcggacacg
     7261 aagaaatatc aaagggtctt caaatgtcgg catggcagcg aatcagttgc cgtctcgggc
     7321 gggttcgcca tccacggggc gctcgccggc gggatcgccg agtttcagct tgagctggcc
     7381 gctcgagccg ttggtctcgc cgtagcgctc gtaggctttc acgatacgct gcacgaggtt
     7441 gtggcgcacg acgtcacgct cgtcgaagtt gacgaagcgg ataccttcca cgccgcgaag
     7501 aacctcaatc gcctcgagca acccgctccg cttggcaatg gggaggtcga tctgggttac
     7561 gtctccggtg acaaccatct tcgagttgaa tccctggcgg gtgaggatca tcttcatctg
     7621 ctcgggcgtg cagttctggg cctcgtccat gatgatgaag ctgtcgttga gagtacggcc
     7681 gcgcatgaag gcgagcggcg caatttcgat gaccgagcgc tcgaggaact tttcgacctt
     7741 gtcaacgtcg agcatgtcgt agagggcgtc gtagaggggc cggaggtagg ggtcgacttt
     7801 ctcctggagc gtaccgggga ggaagcccag ccgctcgccg gcttccacgg cgggacgggt
     7861 gaggatgatc cggctgacct gcttggccat gagcgcggag acggccatcg cgacggccag
     7921 ataggtcttg ccggtgccgg cggggcctat gccgaagacg aggtcgtggc gctcaatggc
     7981 atccacgtag cgccgctggt tgcccgattt cggagcgacg accttcttac cgaaactgcg
     8041 ctggcggccg ctgaggacaa ggtcgcggag ccgcacgccg gggtcctcgg tgaccacacg
     8101 caggtagctg ttgaggtcgc cgttggcgaa cgtatgtcct tccctgacga gggatacgta
     8161 gtcctcgagg atgcgctcgg cgcgagcaac gttggcctcg tcgccttcta tttcgaggca
     8221 gtcttccagc aactgtgtgc tcacattgag gccggtttcg agcaggcgga tattttcgtc
     8281 acgggtaccg aaaagcgatt cgatgccccg gctaatctcg acacttactt tcattggcaa
     8341 tcgagtatgg ctacctccag acgactatgg cggaaaacgg gtgaggcggg cacgacttcg
     8401 cgctcgggca cgtcccctgt ctgtttcaac cgtgtccgcg cgcgcggagg ggtgaacatc
     8461 cagtctgagt atagcagaca gcggcgttag cctcaagaaa ataaaaggtt acagaagctt
     8521 gagagtctgc tggccgagtg tagaccgcag attccagacc gcttgaacgg ccgacggtca
     8581 tttcaagcac cgtcacgctc gagcgcggga aggtgtattc gaattcgggt gcggcggggg
     8641 cgacgctctc gaccgggtgg acggcttcgg ggcgcatctc gtcattcggc tgttggatgc
     8701 tcgtaccggc g
//
LOCUS       DATIFQ010000086         3966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1559227, whole genome shotgun sequence.
ACCESSION   DATIFQ010000086 DATIFQ010000000
VERSION     DATIFQ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559227"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VLE22_03705"
     CDS             <1..971
                     /locus_tag="VLE22_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HSB13536.1"
                     /translation="IGDTPQGLIFSYGVVGGLGVGFAYVTPIATCIKWFPDRRGFIVG
                     LSVMGFGIGSLIFAPLLEALIGRDPALFAVTIPRTFQILSVIFFVLVVGAAQAFRVPP
                     PGWKPEGWNPPAASATARENYGPGEMLRTWQFYVLWLLYFLGTSVGLTAIGESTPLIQ
                     EMAKDAALSGGAALGIMSLFNGAGRLCWGTLSDRIGRNRTVVAMCGVSILACLAFLRN
                     ASGFWEFLTGLCLVGFAYGGYLALMPSFTADFYGSRSVGANYGILFSAWGLCGFIVPG
                     YFASVMERARQAGDLAAGYNEVYATLAVLAAVGGVAAMAARRPSAS"
     gene            complement(984..1850)
                     /locus_tag="VLE22_03710"
     CDS             complement(984..1850)
                     /locus_tag="VLE22_03710"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB13537.1"
                     /translation="MKTTARLGLVALGLMLAGSCAALAQNDPNKQEWIQLFNGKDLKD
                     WAVKINGYDLNDNFGDTFRVENGVLKVVYDKYDKFNSRFGHIFYRRPFSHYIIAVEYR
                     FLGNQVPDAPDWAFRNSGIMVHCQPPETMRKDQNFPISIEVQLLGGPDSGERSTANLC
                     TPGTNVVMNGKLVTQHCVNSTSKTFRGDQWVRAEAMVLGDSVIRHIVNGETVLTYEMP
                     QIGGDAVDNFDPAVKHDGELLTEGYISLQSESHPIEFRKVELLNLAGCMDPKASNYKA
                     YYVKANNAECRT"
     gene            1970..2470
                     /locus_tag="VLE22_03715"
     CDS             1970..2470
                     /locus_tag="VLE22_03715"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HSB13538.1"
                     /translation="MTSAAFTSAGATGFPPLEPVPSLDLERYAGRWYEIARYPNRFQR
                     DCVSDTTATYTLRSDGKVTVLNECREADGKLKSAKGTAKLANENGPASKLKVTFFWPF
                     YGKYWVIDLDLEYQWAVVSEPGRAYLWILSRTPRIDEALYDQIVKRLIERGFDAGKIM
                     RTKQSG"
     gene            2599..>3966
                     /locus_tag="VLE22_03720"
     CDS             2599..>3966
                     /locus_tag="VLE22_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSB13539.1"
                     /translation="MPHILYRRAILVYLAVIVVPALVLLYLGLQSVKRQNEAISALRA
                     SNLRLSQERLEEELERRTALLAETCLREAAAVTGDHARLEQVRRQHTVARHFFVLTDD
                     TVRYPLLRTPPPRLPAGLPAEFHQAEAAEHRENRTAEALAAYRGVLEMETSDPVKALA
                     LSRVARCLQKLRRTNEAAQAWRTLLEKYGDLRDPSYRPYALVAAFELGQERSVYEDIV
                     QGRWELSAGQVDYFLERLERPASASLTNPLDRFEFARAVEDNFHHAGPLRPDEVYRLD
                     LPPYQLYYLLLSPGSNPETLLGLSVNSAWVEKELVPQIRDELGIAEQVPPAGERSLLL
                     YGGATGLVLGVLIMGVVLMIRDVTRETRVNQLRAEFVSGVSHELRTPLTLIRLYGDTL
                     LEGGEFAEEERRGFYRIITRESERLTHLIEKVLTFSRIERGEKQYRLEEGDLAPVVAR
                     TVEPYE"
BASE COUNT          698 a         1133 c         1313 g          822 t
ORIGIN      
        1 agatcggcga cacaccgcag ggactgatct tctcctatgg cgtggtgggc ggcctcgggg
       61 tgggattcgc gtacgtgaca ccgatcgcga cgtgcatcaa atggtttccc gaccggcgcg
      121 ggttcatcgt gggcctgtcg gtgatgggct tcggaatcgg gtctttgatc ttcgcgccgc
      181 tgctcgaagc gctgatcggc cgcgacccgg ccctcttcgc ggtgacgatc ccgaggacgt
      241 ttcagattct gagcgtaatc ttcttcgtgc tggtggtggg cgccgctcag gcgttccgcg
      301 taccgccgcc gggatggaag cccgaagggt ggaaccctcc ggcggcatcc gcgacggcac
      361 gggagaatta cggaccgggc gagatgctgc gcacgtggca attctatgta ctgtggctgc
      421 tctacttcct gggcacgtcg gtcgggttga cggccatcgg cgaatcgacg ccgctcattc
      481 aggagatggc taaggatgcc gcactctcgg gaggcgccgc gctgggaatc atgtcgctgt
      541 ttaacggcgc cggacgcctt tgctggggca cgctgtccga ccggatcggc cgcaaccgga
      601 cggtggtggc gatgtgcggc gtgtcgattc tggcgtgcct ggcgtttttg cggaacgcgt
      661 cggggttctg ggagttcctt acaggcctgt gcctggtggg ctttgcgtac ggcggctacc
      721 tggcactgat gccgtcgttc accgcggact tctacggatc gagaagcgtc ggcgccaact
      781 acggaattct gttttcggcg tgggggctct gcggtttcat cgtgcccggg tatttcgctt
      841 cggtcatgga acgggcgcgc caagcgggcg acctcgcagc gggctacaac gaggtctacg
      901 ccacgcttgc cgtgttggca gcggtgggag gcgtggcggc aatggccgcg cggcggccga
      961 gcgcgagctg agagggtcgg cccctacgtc cggcactcgg cgttattcgc cttgacgtag
     1021 taagccttgt agttggacgc cttggggtcc atgcagccgg ccaggttcag cagctcgacc
     1081 ttacggaact cgatgggatg actttcgctc tggagggaaa tatagccttc cgtgaggagt
     1141 tcgccgtcgt gtttcacggc cggatcgaag ttgtcgacgg catcgccacc gatttgcggc
     1201 atttcgtagg tgagcaccgt ctcgccgttc acgatgtgcc ggatcaccga gtccccgagc
     1261 accatcgcct cggcgcgcac ccactggtcg ccgcggaagg tcttcgaagt tgaattgacg
     1321 cagtgctgcg tgacgagctt accgttcatg accacgtttg tcccgggggt acagaggttg
     1381 gcggtggagc gctcgcccga gtcggggccg ccgagtagct ggacctcgat ggagatcggg
     1441 aagttctggt ctttgcgcat ggtttccggc ggctggcagt gcaccatgat tccgctgttg
     1501 cggaatgccc agtccggggc atcgggaacc tggttgccca agaagcgata ctcgacggcg
     1561 atgatgtagt gggagaaggg ccggcggtag aagatgtggc cgaaccgcga gttgaacttg
     1621 tcgtacttgt cgtagacgac cttcaggacg ccgttttcga cgcgaaaggt gtcgccgaaa
     1681 ttgtcgttga ggtcatagcc gttgatcttt acggcccagt ctttcaggtc tttcccgttg
     1741 aagagctgga tccactcctg cttgttcggg tcattctggg cgagggcggc gcacgatccg
     1801 gccagcatca atcccaacgc cacaagtcca aggcgcgctg tggtcttcat gatgagtcaa
     1861 gtatagcgat gtagttgcgt tgcgagcggg cgtatatctt gaatcggaag ctcgttgctg
     1921 gcatctgttg tatgggatgg ccaaaactgc gttctaccgg ttatttttga tgacatccgc
     1981 ggcctttacg tctgcgggcg cgacgggatt tccaccgctg gagccggtac cgagtctgga
     2041 cctggaacgg tacgcgggcc gctggtacga gattgcgcgg tatccgaacc gtttccagcg
     2101 tgactgcgtg agcgacacga cggcgaccta tacgctccgc agcgacggga aggtcaccgt
     2161 tctgaacgag tgccgggagg cggacgggaa gctgaagagc gccaagggga cggcgaagct
     2221 ggcgaatgag aacgggccag cgtcgaagct caaagtgacg ttcttctggc cattctatgg
     2281 aaagtactgg gtgatcgacc tggacctcga ataccagtgg gcggtagtga gcgagccggg
     2341 gcgagcctac ctctggatac tgagccggac cccgcgaata gatgaggcgc tttacgacca
     2401 gatcgtgaag cggctcatcg agcgaggctt cgacgcgggc aagatcatgc ggacgaagca
     2461 gtcggggtga ggactggacc gagcggtgtc gcccgccgcc gaacaactgt ctgtcagcag
     2521 acaattcatg acattgtttt acgggtttca gaggttcctt tccggtcatt gtggtaatct
     2581 gtaggcggct tggcagtatt gcctcacatc ctgtaccgcc gggccatctt ggtttaccta
     2641 gcggtcattg tggtcccggc gctggtgctg ctctacctcg ggctgcagtc cgtgaagaga
     2701 cagaacgagg ctatcagcgc gctgcgagcg tctaacctgc gcttgtccca ggaacgactc
     2761 gaggaggaat tggagcgccg aaccgcgctg ctggccgaga cgtgcctgcg tgaagccgcc
     2821 gccgttaccg gcgatcatgc ccgccttgag caggtacgcc gccagcacac cgttgccaga
     2881 cacttcttcg tgctgacgga tgacaccgta cggtaccccc tactcaggac cccacctcct
     2941 cgtcttcccg ccggactgcc cgccgagttc catcaggctg aggcagcaga gcaccgggag
     3001 aaccgcacgg cggaggcgct tgccgcgtac cgcggcgtgc tggagatgga gacgtccgac
     3061 cccgtgaagg cccttgcgct cagccgcgtg gcaaggtgtc ttcaaaagct acgcagaacc
     3121 aacgaggcgg ctcaagcttg gcgcaccctt ctggagaagt atggcgatct ccgtgatcct
     3181 tcctaccgtc cttacgcgct cgtggcggca tttgagttag gccaggaacg tagcgtttac
     3241 gaggacattg tccaggggcg ctgggagttg agcgccggac aggtcgacta ctttctcgaa
     3301 cgcctcgaga ggccggcttc cgcctctctt acaaaccccc tagaccgctt cgagtttgca
     3361 cgagcagtgg aggacaactt ccaccatgcg ggtccgcttc ggccggacga agtctatcga
     3421 cttgatctgc caccctatca gctttattac cttctgcttt ccccagggag taatccggaa
     3481 acactgctcg gtttatccgt gaactccgca tgggtggaga aagagctggt cccgcaaatc
     3541 cgtgacgaac tcggcatcgc agagcaggtg ccgcctgccg gtgagcgcag tctcttgctt
     3601 tacggcgggg cgacagggtt ggtcctgggt gtactcatca tgggggtagt cctcatgatt
     3661 cgcgacgtca cccgggagac gcgagtcaac cagcttcggg ccgaattcgt cagcggggtc
     3721 tcccacgagt tgaggactcc tctgaccctg atccggctgt acggcgacac attgctcgaa
     3781 ggcggcgaat ttgcggagga ggaacgccgg ggtttctatc ggattatcac gcgggaaagc
     3841 gaacggctga cccacctgat cgagaaggtg ctgaccttct ctcgaatcga gcgcggcgag
     3901 aagcagtacc ggctcgagga gggagacctt gcgcctgtgg tcgcccgcac ggtagagcct
     3961 tatgag
//
LOCUS       DATIFQ010000087        43929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1942987, whole genome shotgun sequence.
ACCESSION   DATIFQ010000087 DATIFQ010000000
VERSION     DATIFQ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 43929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43929
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942987"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VLE22_03725"
     CDS             complement(<1..956)
                     /locus_tag="VLE22_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HSB13540.1"
                     /translation="MTARIPLVFCSLLGLLLLPPTSRAAENKKGDTGSGQRETIAKPL
                     SAKEQRKRDERLRKELEGPYRKWLNEDVTYIITDEERAAFKRLSTDDEREQFIEQFWL
                     RRDPTPDSSENEYKEEHYRRIAYSNERFASGIPGWKTDRGRMYIMYGPADEVESHPSG
                     GSYERPFEEGGGTTSTYPFEIWRYRYIDDVGTDVLIEFVDPTMTGEYRMTMDPSEKDA
                     LLYVPNAGLTMMEQMGLSSKADRFNRTDGTHLGTGDSPLPSRMNQFERLERFAKLQKP
                     PSIKFKDLEAAVSSKITYNLLPMKVRADYVRVTNSSILTNVTA"
     gene            1292..4219
                     /locus_tag="VLE22_03730"
     CDS             1292..4219
                     /locus_tag="VLE22_03730"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB13541.1"
                     /translation="MGELKAQFWSALLVILTVAALVAAGINFQQQSKFRLPEDGVTWV
                     DRGGNVEALHVNPGSPAERAGLRSGDIVRSISGVPTTTPTDVTRVLVSLGAWRKAEYA
                     VHRAGVEIKVPLIIGERTPTGTLYFQYIVGLAYLALGLFVYFRRGNAHKATHFYLLCL
                     TSFVLYTFHYTGKLNEFDKVMYWGNVAAGLLAPTIFLHFCLTFPEPSRWIRGRWRVAC
                     LYVPSAALFLVFLGVASGTLRVGISAVELRWLLDRSWLALLSGFYLAGAVVLHRRWRT
                     AEDPIIRQQLKWLRNGTLLGVAPFIVFCAAPYTLGAVPGAWMKLSVLALAIVPITWTY
                     AVLRYRLMDVDVIFQQGYAYTLATLCILGIFYGLIFSLGKFEDISPTAAGLLILIAAF
                     VFQPIRAWIKDQLDKYYFYRDRYDYRRTLVEFARELSSETDLNQMLASVADRIQRTIS
                     VERAAFFIEDERAERFRLHMTAGWPGGGPGDTEKLDLRFLTSMPDGPYLFFERTKHPL
                     DIVSRGLPAPVRQTIAELDLTYYLPCKVRGKTVAWLGVGRTDKGDFLTSDDLELLATL
                     AGYVGIAVENSQLYSSLQGKVEENERLKEFNENIVESINVGILAVDLADRVESWNTQM
                     ESLTSIPREMAVGQPLAVLFPAKLLTQFDHSREETGIHHISKFSFRPSLRIAVLDAGD
                     NGTNGQTHDQGRSGSAADESTTETAAEVTVNIAIAPLFSREGKRIGRLILFDDVTERA
                     DLERRLVQADKLSSIGLLAAGVAHEVNTPLAVISTYAQLLAKQIAGDEQKSVLLEKIA
                     RQTFRASEIVNSLLNFSRTSPTEFIETDLGKVIRETLSLVQHQFEKSGVSTALNLEEP
                     LPPIRGNPGRLQQVFLNLYLNARDAMERGGVLTVSAWNGENGVEVEVSDTGRGIAPEH
                     VHRIYDPFFTTKAVQKGTGLGLSITYGIVREHGGVIEVDSRLGEGTRFHLEFPAVRKA
                     VNA"
     gene            4212..5687
                     /locus_tag="VLE22_03735"
     CDS             4212..5687
                     /locus_tag="VLE22_03735"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB13542.1"
                     /translation="MPEKALANGIPSTASKGRILVVDDEADIRESLQFLLFHEGYQVD
                     LASNGGEGIGKIEGGGYDLLLLDLMMPDRSGMEVLRDIRERDPEIPVFLITAYGSVET
                     AVEALKAGANDFFEKPWKNEKLLIEIERMISKRRLESENTQLKRALKQRYSFPNIVGK
                     SERMLRVLDLVAQVAPSRSTILITGETGTGKELIAKAIHANSPRADQMFVAVNSGSLP
                     PELLESTLFGHVKGAFTGAIASRKGYFEIADRGTIFFDEIGTIGAETQAKLLRVIQER
                     EFMPLGSTETMKVDVRILAATNADLKKLVEDGRFREDLYYRLNVINLALPALRERKED
                     IPLLADYFFTKYCRENEKFLASNGRSLLRFEAEAMQFLIDHNWPGNVRELENVVERAV
                     VLAAGQMVPVSVLPEYLLQAGGMRIRRDDSGSLPPDASLFEIVADYERRVIMEHLETA
                     GWSQTDAAESLRVPLSTLNQKIKRLNIEVRKRSEARAERGA"
     gene            6267..7151
                     /locus_tag="VLE22_03740"
     CDS             6267..7151
                     /locus_tag="VLE22_03740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSB13543.1"
                     /translation="MNQPYFIVVLAHSLHGRLRRVHIPHQVLYVVLGLALLGCFSLAG
                     FVSSYARMLWKVANYNSLRTEAESLRVRYQKLQKVHDQTTQQLATLQLFASEVSTAFG
                     IKQRVDGPADIAGEGRLVPTFSESLEQYNFLKSASFSRFNRTYSRAWLTNMRPSIWPV
                     DGRLIGSFGQRSDPFSGEPRFHAGVDLGAPYAAPIRVTADGIVTHAEYSAGYGKLVVV
                     DHGGGVSTYYAHLSRIDVIDGQEIRRGETVGALGATGRVTAPHLHYEVRVGNNPVNPY
                     SFLAKSVFAKNATKDFPF"
     gene            7202..8224
                     /locus_tag="VLE22_03745"
     CDS             7202..8224
                     /locus_tag="VLE22_03745"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HSB13544.1"
                     /translation="MDAALVRLLAVCSAAVLVAWGGRKTSVIPVMTGTAETFLAALTP
                     DQRIKAALAFENEERFNWHYIPRARKGIPLREMTSEQKHLAHALLNAGLSQRGYVKAT
                     TIMSLEEVLRAVENDSGERRNPDGYFFTVFGRPSESGTWGYRVEGHHLSLNFTIAGGS
                     IAGGPTFFGANPAEVRSGPRKGLRALSREEDLARDLLASFREEQIAAVIVDKTAYGDI
                     LTAASRKAALAGQPSGLPAARMTVAQRAKLEALLREYAENLPAELADARMVRIRQAGG
                     EIRFAWAGGTARGEPHYYRIQAPAFLVEYDNTQNNANHIHSVWRDFEGDFGLDLLEAH
                     YRASHR"
     gene            8259..9371
                     /locus_tag="VLE22_03750"
     CDS             8259..9371
                     /locus_tag="VLE22_03750"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HSB13545.1"
                     /translation="MEGKGRRPAGWTRRALLTGLAGVSLPVAADVGHPFPSDRVRFLD
                     RATEFEVLRLTDPSYSSILPHYYARAFAKKRNALLFACDRGGSIQGFQMDLKTGASVQ
                     LTEATGLDASSLTWLPDERSCCYFDGVSLRQLNVANRRSREIYRVPDGWRRGSGFSVS
                     GDGVHAFFMEMQADTCRLRIVGIAKGDAVTVAESSEVMSDPVPRPRRSGVLYRRGGNA
                     LWLVNYDGQQNRPLKTAPGGLGPVHWSPDGKSIIYLNFPEDRRDLNSLREHTPDSNSD
                     ELISKTSQFVHFGLNADGSVFVGTSGSKASPYLLLMLRVTHRELTLCEHRASDPNRVT
                     PVFTPDSQQVVFQSDRDGKLAIYAVRVDRLVEKTDS"
     gene            9404..10204
                     /locus_tag="VLE22_03755"
     CDS             9404..10204
                     /locus_tag="VLE22_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB13546.1"
                     /translation="MVRWIASAVVASITIPCTAATKIVVTVVEKRSGRPVPELKADDF
                     IVLDDRAPRRVESAEFASGLMDVMLLLDTSLLGGAVQPVAENLVAQLQPKEQMAIVAF
                     HSSADLVQDFTASQELLGRAISSVKYGNTPRLLDAIYAAIDGGFQASTFRRVIVLLTT
                     GVEGPSRVSEREVLRLARRNGVSIYPVYVVGQERSMFENLARQTGGASFNLRDLQRDS
                     KSPPGPRIFEVMRSHYTLTLEGNLSLGDRVEVKVKRPEKLLVSGLPLD"
     gene            complement(10266..11141)
                     /locus_tag="VLE22_03760"
     CDS             complement(10266..11141)
                     /locus_tag="VLE22_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13547.1"
                     /translation="MSELTETRTPSRSGGNIQIPILFGLVIAVAAATAYLFFTVDGLK
                     TDLHSMRESVLAELAKVRETSSISTASNQQHLDELRSELENTRKQAAMAAGRARTDAL
                     KHAEQLAKQIQTEQARQQEMVKTELTEVKEAASTANNKITDVSGEVTNVKTEVASTKS
                     ELEKTIADLKSVRGDLGVQSGLIATNGQELAALRTLGERNYFEFKLAKSKQPQRIGDI
                     SVVLKKTDPKRSKYSIDVLADDKKVEKKDKTANEPVQFYVAKARLPYEIVVNEVQKDR
                     ITGYLATPKVQIARK"
     gene            complement(11306..12175)
                     /locus_tag="VLE22_03765"
     CDS             complement(11306..12175)
                     /locus_tag="VLE22_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13548.1"
                     /translation="METDEIYGSSGRTSKILLPILYGAVIALSAATVYLFVQLDKMRT
                     EVTQMRESVLNEVTNLRESSSVTSETNRRHLDALREQLEAARRNAAVAAGQAKTEAQR
                     HAEELAKRLAEEQQKQQQAVRSEISEVRQATSDANTKIEDVSKDVTNVRSEVSSTKSE
                     LDKTIAELKTVRGDMGVQSGLIATNSKELSALRTLGERNYFEFNITRSKEYRKVGDIS
                     VRLRKADTKRNRYTVEVLADDKRVEKKDKNVNEPVQFYVARARQPYEFVVNEVRKDQI
                     VGYLATPKVKTVR"
     gene            complement(12245..12874)
                     /locus_tag="VLE22_03770"
     CDS             complement(12245..12874)
                     /locus_tag="VLE22_03770"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HSB13549.1"
                     /translation="MDFISGNIGWIEVICGPMFSGKSEELIRRLRRAMIARKRVQVFK
                     PSLDTRFSEDEIVSHAEVRMKSEIVPDAAEILSRMDWRTEVVGVDEANFMGPGLIEVA
                     GRLADRGKQVIVAGLDTDYMGRPFTPVPELLALAESITKTLAICVRCGNPAKHTQRLV
                     DSDDLIVVGAAGMYEARCRRCFEPGLPKQEVLEFVRLRRAAEAVSGLEK"
     gene            12988..13386
                     /locus_tag="VLE22_03775"
     CDS             12988..13386
                     /locus_tag="VLE22_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13550.1"
                     /translation="MNSRVLCFMAGIVLLAGCNRGVENKEAVRQAVIDHLANRAGLNV
                     ASMQVDVSAVSFRKDEADATVVFRSKGGAPGQSMTMNYTLERKGGRWVVKGRSESGMP
                     HGAAGTPAPGGQMPQGHPPIGGAKPSEVPK"
     gene            13383..14558
                     /locus_tag="VLE22_03780"
     CDS             13383..14558
                     /locus_tag="VLE22_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB13551.1"
                     /translation="MKRVAILGGGPAGAFAAERLAAGGAKTVVFDEKLAWEKPCGGGL
                     TYKAYSRYPFLIENDTPKKFVTVTQLAAPGAGVAKMALRKPLVVYSRFDLNRMLLARA
                     ERAGAQIEQTRVLGMTPAGRGWRLSTHHGDFEADFCVVATGARNPLRDVGTQLTAADT
                     MLALGYYVQADQEHIDIQFLPQLEGYIWVFPRCGHLSVGICGKGEPAHQLRARLEAYM
                     RERGISWKDAVFYSHLLPSLARTGWRNNRVAGHGWIAVGDAGGLVDPITGEGLYYAMR
                     SGDLASQVVLSGRHSAETMAVAYRAMLTRDFAADLEFGASLARRVFLGRFLLDSVPNR
                     MVQIMRRSPRFCELMQDLFAGTQPYLDLKRRLLSNLHGTLLETAMNFLLSRVVPSET"
     gene            14561..15826
                     /gene="hemL"
                     /locus_tag="VLE22_03785"
     CDS             14561..15826
                     /gene="hemL"
                     /locus_tag="VLE22_03785"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HSB13552.1"
                     /translation="MRRHTSESLFDRAQRSIPGGVNSPVRAFRSVGGKPLFISRGEGS
                     HIFDADGNEYVDYVGSWGPLLLGHRHPAILTALEEAAHSGTSFGAPTEREIELAEAIR
                     DAVPSIEMVRLVNSGTEATMSAIRLARGLTGRDLTVKFEGCYHGHVDSLLVKAGSGVA
                     TLGLPDTAGVPKSFADTTIALPFNSAEAVEQAFREHGARIASVIVEPVAGNMGCVPPK
                     PGFLAALREITERYGALLIFDEVMTGFRVAWGGAQQLYGIRPDLTTLGKVIGGGLPIA
                     AYGGRAEVMAKIAPLGPVYQAGTLSGNPMAVAAGLAMLRHLKAHPEVYTTLERNGERL
                     QAAAPSEITVNRVGSMFTFFFTDQRVTDYDSAKTCDTARFGDYFRFMLDRGVYLAPSQ
                     FEAAFLSAVHTDEDITRTIEAAREFFARR"
     gene            complement(15836..17428)
                     /locus_tag="VLE22_03790"
     CDS             complement(15836..17428)
                     /locus_tag="VLE22_03790"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSB13553.1"
                     /translation="MAPTETADWLIEGARIYTAEPGNPVASAIAVKGGRILAIGDNLS
                     RLAGSSTRRIDAGGASIIPGLIDSHVHMLALGESLENPDFRYVRTVEEVAEIVRKAAS
                     GRTRGEWIQGRNWDQTNWGGRFPTEEPLSRAAPDHPVFLTRVDGHASWVNRKALEIAG
                     ITAKTADPPGGKIVRGANGKPTGILVDTAQGLVERKIPNPTDEQVRRRLERAARECAR
                     LGITSVHDAGVGARELAAYRALIREGRLPVRVYAMIGGEGPLWREYLERGPETGDRLT
                     VRSIKLVADGAMGSRGAAFFEAYSDDPGNKGLVMFSREDVERVARQAAGKGFQVNTHA
                     IGDRANHDVLEAYRAVLGSKNDKRFRIEHAQCVAPGDFELFGKYSVIASMQATHATSD
                     MRWAEARVGPERVKGCYAWRRFLSIGVPVANGSDFPVEEPNPLWGFYAAITRQDRDGN
                     PKDGWFPDQRMTREEALKSWTVTCAYAAFEEKEKGSLAPGKMADFVMLSNDIMRAAPP
                     EILETRVLMTVLGGATVYAEKR"
     gene            17460..18383
                     /locus_tag="VLE22_03795"
     CDS             17460..18383
                     /locus_tag="VLE22_03795"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSB13554.1"
                     /translation="MHDSMAHAVDESAPLEWPAWKTWVSAISAVLLAILFIVSGVWKV
                     TDPLAAAVRMAQAKVPNVLSLPTAIGFGIAEAFAGVLLLVPRFRRWGAWLTGLMLVAF
                     LIYIGVLYNDLRGEDCNCFPWVKRAVGPAFFIGDAIMLAMAVLAGWWVRPSHGLRSAS
                     IIFGAVCVFALVSLGIHLTRQSGVLAPETVTVDGKAFPLREGRVFLFFFNPECSHCNH
                     SASELAKLDWGETKIVGVVTQLAQFGPDFMRETGLKGVVTADVGLLKNAFSFVDVPFG
                     VALENGRQRASFPNVDPEPAAETLRKLGFVN"
     gene            complement(18455..19468)
                     /locus_tag="VLE22_03800"
     CDS             complement(18455..19468)
                     /locus_tag="VLE22_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSB13555.1"
                     /translation="MTGKLKTALAILVAACVLIPTAAIAKDKKKDPDEIGNRDVGRGV
                     NFYSLEKEIALGKQLAQEVEQQAKVVDDPVIGEYVNRVGQNLVRNSDAKVPFTIKVLD
                     SDEVNAFALPGGFFFVNSGLILRAESESELAGVMAHEIAHVAARHGTRQASRGQLMNI
                     ASIPLIFMGGWTGYAIRQGAGLAIPITFLTFSRGFEEEADLLGLQYLYKTGYDPTAFV
                     DFFEKIQTLEKKKPGTMSKVFSTHPMTDDRIKTAQKNIQEILKAKPEYVVNTSEFNEV
                     KTRLSAMHNRRKVDDKDLNRPTLRKAPGGSTAPIEDDKSKGKDKDQQKEDPDERPTLK
                     RRG"
     gene            19567..20712
                     /locus_tag="VLE22_03805"
     CDS             19567..20712
                     /locus_tag="VLE22_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB13556.1"
                     /translation="MGYEKATESEEVPLWKRFRHCFPVTERLVYLNHAGVAPICKPAA
                     DAMSRLTEDALQFGSYHYDSWLATYEGLRAIASRLIGASRDEIAIVKNTSEGIATLAS
                     GLKWRQGDKIVAFTEEFSANYFPWKRLEAQGVTVEWLSVFDTFDKIDRACKGARLLAV
                     SYVQYLSGHRINLEVLGEICNLRGCLFFVDAIQGLGAFPLDVRSSRIHALSADGHKWL
                     LGPEGCGILYIHKNLFDSVEPPMIGWTNFAHSSDYESRDMTLRTDAGRFECGTLNTVG
                     CFGLRASIEFLLSVGVPKIAVAVQLLGDQIVQGVTAKGYQVLGRRTASNGAGIVCFRR
                     SDVDSRLIVRDLKAKGIVAASRQGWVRVSPHFYIAPDDIDRLVEELP"
     gene            complement(20717..21193)
                     /locus_tag="VLE22_03810"
     CDS             complement(20717..21193)
                     /locus_tag="VLE22_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HSB13557.1"
                     /translation="MAGEAPPRLRVLCLGNEILGDDAFAIRVAGRLNELSIEGVEVVT
                     SEASGFHLLDDILCAASLAVVDVIETRKAPPGTLYEFREDDISPLPGEFPHGIGLFQV
                     LALGRKLGLEVPPEVLFLAVEPHDCLTVGGAMHPAVLAAVPRTVARIQELASASVA"
     gene            complement(21202..22707)
                     /locus_tag="VLE22_03815"
     CDS             complement(21202..22707)
                     /locus_tag="VLE22_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HSB13558.1"
                     /translation="MSVETTPTRRVTIDPITRLEGHGKIDIFLDDAGQVERAYFQVPE
                     LRGFEKFAEGRPAEDMPQITSRICGVCPSAHHMAATKALDDLYQVEPPPAGKKIRELF
                     YNTFMAEDHALHFYFLGGPDFVVGPNAPAAERNVLGVVAKVGLEAGKKVIGFRRKLRD
                     LMTLVGGKAIHPVLGLPGGVAKRITKDDQEQFRRVAGESVDFCLFTLDLFDQVVLKNS
                     DYVNLIVSDTYTHRTHYMGTVDERNRVNFYDGKLRVVSPEGKELMKFPQNQYLDYVAE
                     HVEPWSYMKFCYLKPIGWKGFVDGADSGVYSVAPLARLNVSDGMPTPLAQAAYEQFFA
                     TLGGKPVHHTLALHWARMIEMLCAAERMKELAEDPELTDPNVRKLPTATPKEGIGVIE
                     APRGTLIHHYQTDEKGLIRKANLIVATQHNAARMAMSVEKAAKGFVTRDKISDGVLNM
                     VEMAFRAYDPCLGCATHSLPGSMPLFVRVRDANGATTATLRRDNDGSVRRD"
     gene            complement(22711..23682)
                     /locus_tag="VLE22_03820"
     CDS             complement(22711..23682)
                     /locus_tag="VLE22_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HSB13559.1"
                     /translation="MSEKAKVAFYWCASCGGCEEAVVDLAEKILEVSAAVEIVLWPVA
                     LDFKRKDIEAIPDGGIVATFLNGAIRSTEQEEMAHLLRRKSKVMVALGSCSQLGGIPG
                     LANLWDRSAILEAVYKGSPSTVNPEGTTPQTAYKDNGRTPTLPMLYNTVRTLDQVVDV
                     DYYIPGCAPAAKLVLDAVETLLSGNLPPKGTVLAPDRALCDECPRRDTRPDKLELAEF
                     KRPHQIIADPEKCLLAQGLLCLGPATRGGCGGLCTKGNMPCTGCYGPTSRVRDYGGKA
                     LTALASIIEAKDDTDIEKVLAGIPDPVGTFYRYSLPASLLKRKTLEA"
     gene            complement(23679..24128)
                     /locus_tag="VLE22_03825"
     CDS             complement(23679..24128)
                     /locus_tag="VLE22_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HSB13560.1"
                     /translation="MPENYEPKIVAFFCNWCTYLAADLAGTSRMKYAANTRVIRVMCS
                     GRVDPQFVLEAFAKGADGVLIGGCHPGDCHYQEGNYKALRRYYLLKRTLREMGIEEER
                     FRLEWISAAEGDRVRTVVNDMVQKLRALGPRKMNPAIEPELEEVAAS"
     gene            complement(24121..26115)
                     /locus_tag="VLE22_03830"
     CDS             complement(24121..26115)
                     /locus_tag="VLE22_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HSB13561.1"
                     /translation="MSERNGIVRIGVYVCHCGTNIAAIVDCKAVAEYAGGLYHVTVSR
                     DYKYLCSDPGQELIRQDIRDHKLNRIVVASCSPHLHEHTFRTAAEGAGLNAFYVHMVN
                     IREHNSWVHENREAATKKAQDMVRAAVRRVVFHQSLEKVRVPVHPDVLVVGGGIAGIH
                     AALTLANAGKKVYLVERQPTIGGHMAMFDKTFPTLDCAACILTPKMSSVKAHPNITLW
                     AHSDVTKVEGYVGNYKVEVARRPRYINEDLCSGCLECIEACVYKEPKFIDEFNMGLGK
                     RKPIYIPFPQAVPQMPLIDIDTCIQFKSGKCKKTCVQACGDRHAIDFSQKEEIRNIEV
                     GTIIMATGFKAFDARRTPYYGYGTYPNVYTSLEVERLVNASGPTAGDVVLRDGRKPKT
                     VGIIHCVGSRDEGTNRWCSRVCCMYSLKLAHLLKEHTDAEIFNFYIDIRTPGKGFEEF
                     YQRLLSEGVHFIRGRVAEVTDWALDPTEENKLVLRVEDTLLGTVRRIPVDMVVLSVGI
                     EPHEDAQDVRRLFNFGCGSEGFFLERHPKLAPVSTFTDGIFVAGCCQGPKDIPDTVAQ
                     AGAAAAEALALIDTGFVEQEPNTAYVIEDECSGCKTCVPMCPYSAVYFNADTKRAGIH
                     QVLCKGCGTCVAACPSGSIKQHLFEDDQIFEEIEGVLTYA"
     gene            complement(26108..27034)
                     /locus_tag="VLE22_03835"
     CDS             complement(26108..27034)
                     /locus_tag="VLE22_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HSB13562.1"
                     /translation="MTPEQAAKTSKGYLYFPGCSLRATGVAYEESLVELFRLLNIPLV
                     ELPDWNCCGATSYMCIDEGSAFLLSARNLSLAHTVNSRDLLAPCAACYLVLKKTQDYA
                     AKHPKIRTQVEESLARGGLPPFDGVKVRHPLEMLYNDVGRDTLKSNVIRRWRGGRIAC
                     YYGCQAVRPYDEVDTAYNPMRMDELLQDVGAPTVDYALKTKCCGGSLTGTVHEVGVRL
                     NYILLKEAARKGAEAIVTICPLCQFNLDAYQAEIRKATNEPIDMPILYFTQVLGYALG
                     GDPKKLGLHRGISGRDLIKRWFPAHEEVEAYV"
     gene            complement(27031..27615)
                     /locus_tag="VLE22_03840"
     CDS             complement(27031..27615)
                     /locus_tag="VLE22_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSB13563.1"
                     /translation="MLALIRRKMKFQREADPEWAEQISKAPGCDRVFSCIQCGTCSGT
                     CPLSIYMDFTPRRIIALVREGFRTDALSCQTIWLCASCYSCTVHCPQNVHITDVMYSL
                     KREAIKHKLYPSRFPIPVLAREFYEIVRRRGRNSEFWLVLRMAMKSNPLVLLTMAKTG
                     WGLLRTGRMSLKSERITRIRELQNELADPQEVSR"
     gene            complement(27731..29713)
                     /locus_tag="VLE22_03845"
     CDS             complement(27731..29713)
                     /locus_tag="VLE22_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HSB13564.1"
                     /translation="MIDAESPRIGAYVCHCGTNIAGTIDVYELARYAGTLPGVTVARE
                     YKYMCSDPGQELIKNDIRDHGLTRIVVAACSPCLHEKTFRKATAEGGINPYFFQMVNV
                     REHASWVHDDKLAATEKAKDMVRAAAARVALHRPLERRQVPIHPDVLVVGGGIAGIHA
                     ALTLANAGKRVYLVEREPTIGGHMAMFDKTFPTLDCAACILTPKMTAVRSHPKITLWT
                     LSEVAAVEGYVGNYKVTVRRKPRYVIEELCVGCMECIDACVYKQPRFEDEFNQGLSKR
                     KPVYIPFPQAVPQVVVIDPDTCIEFKSGKCKKTCIEACGERNGIDFQQKERLETIEVG
                     AIILATGFQTFDAKRIPYYGYGTYPNVYTALEVERLVNASGPTGGEVILRDGRRPQSV
                     GIIHCIGSRDEHTNRYCSRVCCMYSLKLAHLIKEHTGAQIYNFYIDMRTPGKAFEEFY
                     NRVAEEGVQFIRGKVGDVYPEENGAGRLIMQAEDTLLGQVRKIPVDMVVLAVGLEAQP
                     DAQEVRRRFNITCANEGFFLERHPKLAPVNTFTDGVFLAGCCQGPKDIPDTVAQAGAA
                     AAEAFSLIDAGFIEVEPNTAYVLEEDCSGCKTCVGLCPYSAIRFLEEKKKAHINEVLC
                     KGCGTCVAACPSGSIGQNLFEDDEIFEEIRGVMAYV"
     gene            complement(29710..31563)
                     /locus_tag="VLE22_03850"
     CDS             complement(29710..31563)
                     /locus_tag="VLE22_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HSB13565.1"
                     /translation="MADPATSVYFGVPGYAVLWALTLVSFFLFGRRVERYIGVLRKAR
                     PEVRWNEPWRRMRMFAIYVLGQKKMFGERAIGAAHFLIFWSFVFYASSFFWNLIRGLL
                     PFLPIPYTDSVRWMAAVLELFGVLGLVALIVAAVRRYVFTPPRLERSKDASLILTLIA
                     VVLITSLAGSMTESPQIYAAMWWLHMATVLGFLAYLPYSKHMHLLASPFGVFFGTLRE
                     GGIPAASEGASRREEFTWRELFNGLACAECGRCDRSCPCFDAGLPLSPKMLMHHVKEL
                     VRNGGGNGDFAGGVVKAEEIWACATCYSCMERCPVFNEHIPVIVEMRRHLVSKGEVEP
                     RVQEALMNFTRYGNSFGTSPRARAKWIQGLDFKPKDARKEPVEYLWFTGDYASYDPRL
                     QPVTRSAARLFRKGGVDFGILYEEEQNSGNDARRVGEEGLFEMLRDKNLKALGKAKFE
                     RIVTTDPHTYHALKNEYGEGNGAVLHYTELIDELLKSGKLPLERRLEAAVTYHDPCYL
                     GRYNGIYDAPRRVLSTLGAKLVEMPRNRARAHCCGAGGGRIWMEDTPGVKERPAENRV
                     REAATTDGVSTLAVACPKDLVMFQDAVKTTGLEGRLAVRDLIELVEECTAQ"
     gene            complement(31570..32541)
                     /locus_tag="VLE22_03855"
     CDS             complement(31570..32541)
                     /locus_tag="VLE22_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HSB13566.1"
                     /translation="MAGNIWVVAEQWRGEISEVSFELLALGREVADQLGVPLEAVLMA
                     EGRKESAGALGKADVVLYADHSSLAEPIPDVCAEALVQLAQERRPRAILVALTNVSMG
                     IGTLAAAKLSAPGINFCTDLKVADGRIEAQCVLYGGKMEATVTAHGEPAILGIWPGAR
                     SPEKGRATQAPAVEDVTVTLPEPRVRLKRYLEPEAGDIDISQQDALVAVGRGIQSKDN
                     VALAEELAETLGGAICGSRPVIDQGWLPLSRQVGKSGLSVKPRLYVAAGVSGAPEHVE
                     GMKNASLIVAINSDPQAPIFNVAHYGIVGDAMDVLPALTEAVRARKG"
     gene            complement(32555..33340)
                     /locus_tag="VLE22_03860"
     CDS             complement(32555..33340)
                     /locus_tag="VLE22_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13567.1"
                     /translation="MKILVLLKMVPDVVEELEVAPDGKGLDLEFLRMILSEPDDHALE
                     EALILKERYGGSVTVAALDAPDVDDVLYTALAKGADRVVKVADIEPGTTTRMAAAALS
                     GVLRESTGLLPADLILTGSQAIDDLDGLIAPLLAHSLELPYVGIVTQVRADEAAKTAT
                     VIKEYPGGVRGEFETPLPVVLGIQSAEKPPRYVPVAKVRAAMKSQPIETAGALTAADA
                     GGPLAELVAMNKPMVTGAAEMIEGSPEDIAGRLCGIFAERGVL"
     gene            complement(33347..34555)
                     /locus_tag="VLE22_03865"
     CDS             complement(33347..34555)
                     /locus_tag="VLE22_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSB13568.1"
                     /translation="MLGTAPVETEPTCQITLGNLYDTPEGKRLLTCIQCGVCAGTCPY
                     GDVMEYPPRKMIAMLRASLLEKVFTSESLLACVACYACMAKCPRDIRLTEVLLPLMKE
                     QTLVHLPDLPAELKKALENTLRYGNPMGESSRKRAAWVKTADVPIRILSDYRQPVDVL
                     WFVECYTSFYPRGQDNSRATAKLFHAIGSDFAILGNEEKCAGECGRLTWEPGLFETLT
                     DYNMAVFQKYEFNRLVTSCPHAHNAFMNRYRKLGFWWELEHTVPYLARHLDRLKPMLK
                     RKLNYTVTYHDPCCMGRYNDVYEEPRQLLMAIPGIKLAEMKHNRMNAVCCGGGGGGMW
                     LDTYYKAKGYDRLSERRIKEAIATGADVLAVCCPYEVSRFEDALKVLGHDNRMIVRDV
                     TELLAESLEV"
     gene            complement(34867..35328)
                     /locus_tag="VLE22_03870"
     CDS             complement(34867..35328)
                     /locus_tag="VLE22_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13569.1"
                     /translation="MRPVGADKKASYQANQSSPKQLEVVLFPYTANTKELPDDTVFDA
                     IKANNVGVFGIKPFADNALFVGDGTLNGPYAKEDDRRARLAIRYILSNPAITAPIPGM
                     INSHQVDNMALAVQEHRKLDLKEKAELEKACKEMWANLSPDHKWLRNWEYV"
     gene            35616..35837
                     /locus_tag="VLE22_03875"
     CDS             35616..35837
                     /locus_tag="VLE22_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSB13570.1"
                     /translation="MGGKANLPELTPREKQVYDLVILGKPDKDIASDLGISLRTAKFH
                     VSNIYRKYGVSSRIQLILRERNMSLNTSH"
     gene            36091..38058
                     /locus_tag="VLE22_03880"
     CDS             36091..38058
                     /locus_tag="VLE22_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13571.1"
                     /translation="MSYKRCTSAAVWIAGLLAEAAAAAVILGILSTAQAAVPYPPPTT
                     WKGVNYSPRRHTYFRMLYDWYSWDSTAGKYVHQMVDADLTRLSQNGYNLLHLYLWDQE
                     LLRSVNAAEPAGFLSAPSDPSGSPNNQWGALNDFVTKAESKGLFVALHFASGRFIHRM
                     QGNPGGTPDQVATEFANWTEKFIRYLNSTNPHNNVLLWGLAYSIAPVPGDTTGDWSTT
                     WKLAYKKVDDYARMYSPHPSVLGLIGTNLYMDQLRPDGQPWDGFIYPRSQGYTWNWQE
                     AQRMAKTMRDLLTSAYGTQKDPDIYMMQLYHPNSYDLLLHLSSLSSGYVANGIPAPVA
                     KIFVVEFATSSSLNSPPNGNNIPYYGDAQTPTTTVSGHSQWLNNTLCAYRSAGLQKYA
                     YWSMFDPYTMWTGSPWYVTGQDLAWSGFWGLSFEPEASGDKPSWSLLSSFYLNGSINC
                     PSPGQSNAPAVVSLTSSSNYYTVAQPVRLTWTAADVASFSLNRGHGASYSCSPEQTGQ
                     VSSDVAGSCAYTDASAFSSTGSQTVTLTGYNVWGSPQNASASVTIGLGPIVNAATDQN
                     YTYTINAYDTIIVWGNGFSLTGGNTIQLTRAGYPDVWMYNGDGHYFWDHSHFQINASL
                     DGRAATGQWTLYVRNGYSGTPSAAYTIMINP"
     gene            38110..38871
                     /locus_tag="VLE22_03885"
     CDS             38110..38871
                     /locus_tag="VLE22_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB13572.1"
                     /translation="MRTFLALFLGCVFLPVCLSAQTGGRVSGAILSDETGAPIVGATV
                     VAVQQSSNFGKVGPAIFRSTTELDGSYAIANVPSGDYLLCLYEAGDYLDPCQWGPATN
                     ATIATGTTSLDIRVRKGVRLAVRVGDAENLVAQYKKSSPDASAIPGAFIRITVTDTQG
                     KMRTLPLTYASERLYEYSTLVPPDTDLTLNVSSSFFMLADQSDNPIGEQGYSAPLRLS
                     IPKPPDPRIRLPFAFLDRRATVIPVKIRAAASGAP"
     gene            38930..39790
                     /locus_tag="VLE22_03890"
     CDS             38930..39790
                     /locus_tag="VLE22_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13573.1"
                     /translation="MTSDRTWSVLFLTILASCFTVEAEIRGVVATSGASFELGLPAKG
                     SIASIFCAGIEVDGIVAADSLPLPRTLAGVRVWVGGAPAPLFAVAALQGFQQINIQVP
                     HEAEFGAEAAEVVVEQNGQSGTATVPLRLSTPGDFFRFAGSNLGIFQHASDYSVVTPE
                     NPARPSEAIIAYLTGMPGTEPTVPTGEPAPFDPLAVVPQYRYANAYEHYEVFIGTSSL
                     GVEPSFVGFTPGLVGVYQLNFVVPSDRPSAMAQLVLVRISCGGFFGSCPGNPLFTRTY
                     KSTPVTIPIK"
     gene            complement(39989..41083)
                     /locus_tag="VLE22_03895"
     CDS             complement(39989..41083)
                     /locus_tag="VLE22_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB13574.1"
                     /translation="MSDTPMTRRGFLAGGAAVAGVAAANATPVNTRSILNYNPNMEYR
                     KLGKTGMMVSAVCLGGHWKRVHTMLGGKLKTSVGYSENDMENIQDSDFLKSRDAVVSR
                     CMEVGINYIDACAGPEIMAYSRVLRGRRQKMYLGYSWHVRESRYMEWRAGTKLLAGLD
                     EGMKEAKLDYVDIWRISLPMDMIQSLDELLGVEQGAMEALEKAKKQGKVRFTGISTHN
                     RVWLKSLIEQYPKQLEVVLFPYTANTKELPDDTVFDAIKANNVGVFGIKPFADNALFV
                     GDGTLNGPYAKEDDRRARLAIRYILSNPALTAPIPGMINTHQVDNMALAVQERRKLDL
                     KEKAELEKACKEMWANLSPDHTWLRNWEYV"
     gene            41176..43560
                     /locus_tag="VLE22_03900"
     CDS             41176..43560
                     /locus_tag="VLE22_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HSB13575.1"
                     /translation="MRRFILSILFAACVLRAALPTPESYFGHRMGADRRVLDWSRVVS
                     YFQELDRSSDRIVVKELGKSTEGRPFLAAFIASPATLRDLDRFRSIQKRLADPRKTSR
                     ADAEPLIGRGRAVVMITCSIHATEIASSHTAVEFAWRLLTDDSPKVRAILDNVILILV
                     PSLNPDGMDLVADWYRKTLGTPFEGTSPPHLYQKYTGHDNNRDWYIFSQQETRLVVSQ
                     LHNAWHPHIVYDVHQQGPNASRMFVPPWLDPVDPNIDPLILQECNLFGMGMAAGLTGA
                     GKTGVVVNALYDFWTPARHYQAYHGGLRILSESASARIATPVTVRPDQIDRTALGYNP
                     RESSWNYLEPWLGGEWRLRDIVDYQSIAWESLLTQAASCRETLLRNFYCINQRAAART
                     SPYAFVVSASQSDPGAARKLLETLVFGLVEIGRARGAFEAVGKRYPASSYVIRMQQPF
                     SSFAKTLLERQRYPDLRVYPGGPPRRPYDVTAHTLPLLMGVAVDSVEGPFDAPLDLVS
                     RFEPPPKNRPILPASDTDSWREVNRVWRAGGTVWRDPATGDFYTALRPGLIQLHRPRI
                     GLYRSHVPSMDEGWTRWLLEEFGFAYESVGNTDIEASSLRPRFEVIVFPDQSSSEIEE
                     GFRKGEMPDEYCGGLGDSGAEALRAFARDGGTLIFLNHSTAWARAHLGLDVVDLLEGL
                     PNTEYYSPGSLLNVTLESKSPLTLGLPERIAIWSQGSPAWRAPDATVVARYSQSDMLA
                     SGWLLGEKRLSGQAALVDLPIGSGRVILFGMRPQYRAQSYQAFKLLFNALLLGA"
     gene            43617..>43929
                     /locus_tag="VLE22_03905"
     CDS             43617..>43929
                     /locus_tag="VLE22_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13576.1"
                     /translation="MSPDLLHLHTYFLFPFSIDKQAVLEDHRDLWAKYPRWIDGLDEW
                     IAVHRTRASSTVAQRLGPWRRAAYSRFDLDSSAYQDMVFFHPIVRRVFFDTTDAAGGE
                     KE"
BASE COUNT         8032 a        14048 c        12773 g         9076 t
ORIGIN      
        1 gcggtgacgt tggtcaggat ggaggagttc gtgaccctca cgtagtcggc tcgaaccttc
       61 atcggaagaa ggttgtacgt aatcttggaa ctgacggcgg cctcgaggtc cttgaacttg
      121 atggaaggag gcttctggag ctttgcgaac cgctcgaggc gctcgaactg attcatcctc
      181 gacggcagcg gggagtcacc ggtgccgaga tgggtgccgt cggtacgatt gaaccggtcc
      241 gccttcgaag acaggcccat ctgctccatc atggtgagac cggcgttcgg aacgtacagc
      301 agggcgtcct tctcggacgg gtccatggtc atgcggtact cgccggtcat cgtcgggtcg
      361 acaaattcga tcaagacgtc ggtgcccacg tcgtcgatat accggtagcg ccagatctcg
      421 aacggataag tcgaggtggt gccgccacct tcctcgaacg gtcgctcgta gctaccgcca
      481 gaggggtgag attccacctc gtcagcgggg ccgtacatga tgtacatgcg cccccggtcg
      541 gttttccacc cgggaatccc cgaagcgaac cgctcattgc tgtaagcgat ccggcggtag
      601 tgttcttctt tgtactcgtt ctctgaggag tcgggcgtcg ggtcccgccg aagccagaac
      661 tgctcgatga actgctcgcg ctcatcgtcc gtggagaggc gtttaaacgc ggctcgctcc
      721 tcgtcggtga tgatatacgt gacgtcctcg ttcagccact tgcggtaggg gccctcgagc
      781 tcctttcgaa gccgttcgtc ccgttttcgc tgctcctttg cgctgagagg tttggcgatc
      841 gtttctcgct gcccgctccc cgtgtcgccc ttcttattct ctgccgcgcg agaagtgggc
      901 gggagcaaaa gcaagccgag aagcgagcag aagaccaaag gtattcttgc cgtcatagcc
      961 ttcaccgttc ccagtccata ctggtactgc gtctcttcag ggagctacac caattgtagg
     1021 ctccggcaaa agggggcgtc aagccaaaag gagcggccgc gtgtgaacac ttcggactgg
     1081 cggccagcct cgcggagcgc acactgcccc tacacttccg cgtagtgtaa ttctcccaaa
     1141 tttcagattc gcgcaaccgt tattcgcgtc cggagccaca aaaatgctgt tatctttaga
     1201 agtaagcagg atgctgaggc tggcagtcaa cttgctcagc agtgggtggc atagtgtgga
     1261 gaaccggcaa cagcctcggt ggagtcgccc aatgggggaa ctgaaggccc aattctggag
     1321 cgccctcctc gtcatcctga cggtggctgc cttggttgcc gcggggatca actttcagca
     1381 acagagcaag ttccggctcc cagaggacgg tgtgacgtgg gtggaccgcg gtgggaacgt
     1441 cgaggctctt cacgtgaatc cgggaagccc cgcggaacgg gctggtctga ggtccggcga
     1501 catcgtgcgg agcatcagcg gcgtgccgac gacgaccccc acagacgtga cccgggtgct
     1561 ggtcagtctg ggggcatggc ggaaagcgga atatgcggtc caccgagccg gggtggagat
     1621 caaggtgccg ttgatcatcg gcgagcgcac tcccacgggc acactatact tccagtacat
     1681 cgtcgggctc gcgtatctcg ctcttggact cttcgtctac ttccgccgcg gcaacgcgca
     1741 caaggcgact cacttctacc ttctgtgcct gacttccttt gtcctctaca ctttccacta
     1801 caccgggaaa ctgaatgagt tcgacaaagt gatgtactgg ggcaatgtgg ctgccggtct
     1861 gcttgcgccg acgatcttcc tgcatttctg cctcacgttc ccggagccga gccgttggat
     1921 ccggggacga tggcgagtgg cttgcctgta tgttccatca gccgccttgt tcctggtgtt
     1981 ccttggggtg gcgtcaggca cgcttcgggt ggggatttcc gcggtggaac tgcgttggct
     2041 cctcgaccgg tcctggctgg cccttctctc ggggttctac ctcgccggcg ccgtcgtgct
     2101 ccaccggagg tggcggacgg cggaagatcc gatcatccgg cagcaattga agtggctgcg
     2161 aaatggaaca ctgctcgggg tggcgccatt catcgttttc tgtgcggctc cttacacgct
     2221 gggcgcagtt cctggggcct ggatgaaact gtccgtcctg gcgctggcaa tcgttccgat
     2281 tacgtggacc tatgcggttc tgcggtaccg gctgatggat gtcgatgtca ttttccagca
     2341 ggggtatgct tacacacttg cgaccctgtg cattcttggc atcttttatg gcctgatttt
     2401 ttctctggga aagttcgaag acatcagtcc aactgcggcc ggtctcctga ttctcattgc
     2461 ggcattcgtg ttccagccca tacgggcgtg gatcaaggac cagcttgaca agtactactt
     2521 ctaccgggac cggtacgact atcgccggac gctagtcgag ttcgcccgtg agctgagctc
     2581 ggaaaccgac ctgaaccaga tgctggcttc cgtggcggac cggattcagc ggactatctc
     2641 cgtggaacgg gccgcgtttt tcatcgagga cgaacgcgcc gaacgattcc ggctccacat
     2701 gacggccgga tggcccggcg ggggaccggg cgataccgaa aagctggatc tgcggttcct
     2761 gacatccatg cccgacgggc cttacctttt cttcgagcgg accaagcacc cgctggatat
     2821 cgtttcccgc gggttgcccg cgccggtgcg ccagaccatc gcagagctgg acctgacata
     2881 ttacctgccg tgcaaagtgc gaggaaagac ggtcgcctgg ctgggcgtcg gccgcaccga
     2941 caaaggcgac tttctaacta gcgacgacct ggaactgctg gcgaccctcg cggggtatgt
     3001 cgggatcgcg gtggagaact cccagctcta cagctcgctc caggggaagg tcgaagaaaa
     3061 cgagcggctg aaagagttca acgaaaacat agtcgagtcg atcaacgtcg gcattctagc
     3121 ggtggacttg gcggatagag tcgaaagctg gaacacgcaa atggagagcc tgacctccat
     3181 tccccgggag atggcggtcg ggcagcctct ggccgtgctt ttcccggcca aactgctgac
     3241 gcagttcgac cattcccggg aggaaaccgg gattcatcac atctcgaagt tctcattccg
     3301 cccgagtctg cggattgcgg tgctcgacgc cggcgacaac gggacaaacg gtcaaacgca
     3361 tgaccaaggc cggagcgggt ctgccgcgga cgagtcgacc acggaaaccg ccgcggaggt
     3421 cactgtcaac attgccatcg cgccactgtt ttcccgggaa ggaaagcgga tcggccgctt
     3481 gatcctgttc gatgacgtga ccgagcgcgc cgatctggag cggcggctcg tccaagccga
     3541 taagctcagc tcgatcgggt tgcttgccgc aggcgtggcg cacgaagtca acacaccgtt
     3601 ggcggtaatt tcgacctatg cgcagctgct tgcgaagcag atcgcgggcg acgaacagaa
     3661 atcggtgctg ctggagaaaa tcgcacggca gaccttccgc gccagcgaga tcgtcaactc
     3721 cctgttgaac ttctcccgga catcgccgac cgagttcatc gaaacggatc tcgggaaggt
     3781 gatccgcgag acgctctcgc tggtccagca ccagtttgag aaatcgggcg tctcgaccgc
     3841 gctgaatctc gaagaacctc ttccgccgat ccgaggcaac cctggccgcc tccagcaggt
     3901 tttcctgaac ctgtatctga acgcgcgcga cgcaatggag cgggggggcg tcctgacagt
     3961 cagcgcgtgg aacggagaaa atggagtgga ggtcgaggtc tctgacaccg gacggggcat
     4021 cgctccagag cacgttcacc ggatttacga tccgttcttt acgacgaagg cggtccaaaa
     4081 gggcacggga ctcggactct cgattacgta cggcattgta agggagcatg ggggagtgat
     4141 cgaagtcgat agcaggctcg gtgaaggaac gcgattccat ctggagtttc ccgccgtcag
     4201 gaaggcagta aatgcctgaa aaggcgcttg ccaacggcat tccatccact gccagcaaag
     4261 gtcggattct cgtcgtcgac gacgaggcgg acatccggga aagccttcag tttctccttt
     4321 tccacgaggg gtaccaggtc gacctggcat cgaacggcgg tgagggaatc gggaagattg
     4381 aagggggcgg atatgacctc ttgctgcttg atctgatgat gccggatcgg tccggaatgg
     4441 aagtgctgcg cgacattcgg gagcgcgatc cggagatccc ggtttttctg attacggctt
     4501 acggctcggt ggagacggcg gtcgaggcat tgaaggccgg tgccaacgat ttcttcgaga
     4561 aaccctggaa gaacgaaaag ctgctgatcg agatcgaacg aatgatctcg aagcggcggc
     4621 tcgagagcga aaacacccag ctcaagcggg ccctcaagca gcgttacagc ttcccgaaca
     4681 tcgtcggcaa gagtgagcgc atgcttcggg tgctcgatct ggtggctcag gtggcgccga
     4741 gccggtcgac cattctcatt accggagaga cgggcactgg caaggaactg atcgccaagg
     4801 cgattcatgc gaactcaccg cgagccgacc agatgtttgt cgcggtgaac agcggctcgc
     4861 tgccgccgga gctgctcgaa tccacgctgt ttggccatgt caaaggagcc tttaccggag
     4921 cgatcgcttc ccggaaagga tacttcgaga ttgccgatcg gggaacgatc ttctttgatg
     4981 agatcggtac gatcggtgcg gaaactcagg caaaactgct ccgggtgatt caggagcgcg
     5041 agttcatgcc gctgggttcc accgagacga tgaaagttga cgtccggatc cttgccgcca
     5101 cgaacgccga cctgaagaaa ctggtcgagg acggaaggtt ccgtgaggac ttgtactacc
     5161 ggctcaacgt catcaacctc gcgttgcccg cgctcaggga gcggaaggaa gacattccgc
     5221 tgctggcgga ctacttcttc acaaagtatt gccgcgagaa cgagaagttc ctcgcgtcaa
     5281 acggccgatc tctcctccgg ttcgaggccg aggcgatgca gttcctcatc gaccacaact
     5341 ggccgggaaa cgtgcgggaa ctggagaatg tcgtcgagcg cgcggtggtc ctcgcggccg
     5401 gacagatggt gccggtgagc gtgcttccgg agtacttgct gcaggcggga ggaatgcgga
     5461 tccggcgcga cgacagcggc agtctgccgc cggacgcatc gttgtttgag atcgtggcgg
     5521 actacgagcg gcgagtgatc atggagcacc tggagacggc cggctggagc cagaccgacg
     5581 ccgccgagag cctccgcgtc cccctctcaa cgctcaacca gaagatcaag cggttaaaca
     5641 ttgaggtgcg gaagcgctcc gaggcgcggg cggaacgcgg ggcctgaggg gccctcccgt
     5701 cctcaaggaa acgaaaaccg gcgctgccct gcggtaaaag cggttctagc gaaggataac
     5761 gttccgacgg tctttgtgcc tccggcataa cccttacgcg tcagccgtat caatccaaga
     5821 cgaagcgctc tctccgacgc gggcgcggta atgcggtaat agagagttgc gcctcaggta
     5881 ttcgatggaa aaaacgggaa ccccctccct ggtgccaacc gtgccatgag caataaaatg
     5941 cttgttttta atatattaca gatccaagga gccgacgttt ccgatggccg tgcgcgtgcg
     6001 caattcgaca gccgacgtac gcggagaccc aaactcagcg cctaaggtac tagaacttaa
     6061 cgacctctag ctggtactga accgtcgttc ggttgacgcc gccactcgtc ccggacaccc
     6121 gccgtgcagt ttgggcttga caggccgggc gggctgctga taaagttaca gtttcgcttg
     6181 taaatcatag tccttcggtt ggcctgccag cggttgtgat ttcgttttgg gctgatatgc
     6241 tgcagtggtc cgacccgagg agacgaatga atcagcctta tttcatagtt gtacttgcac
     6301 attctttgca cggcaggctc cgccgcgttc acattcctca ccaagtcctc tacgtagtgt
     6361 tgggtctcgc gttgctcgga tgcttctccc tggccggatt tgtttcgagc tacgcgcgca
     6421 tgctctggaa ggtcgccaac tacaactcac tgcgcactga ggcggagtct ttacgcgtgc
     6481 ggtatcagaa gttgcagaaa gttcacgatc agacgacaca gcaactggca acgctgcaat
     6541 tgttcgccag cgaggtgtcg acggcgttcg gaatcaaaca acgggtggat ggcccggcgg
     6601 acatcgcagg cgagggccgc ctggtaccga ctttctctga gtctttggag cagtacaact
     6661 ttctgaagag cgcaagcttt tcccgattca acaggaccta ctcccgcgcc tggctgacaa
     6721 atatgcggcc cagcatctgg ccggtggacg gccggttgat cggttcgttc ggccagagga
     6781 gcgatccgtt ttcgggagag ccaaggttcc acgccggcgt tgacctcggg gcgccgtacg
     6841 cagctccgat tcgcgtcacg gcggatggca tagtgacgca cgcggagtat tcggcaggat
     6901 acggaaagct ggttgtggtg gatcacggtg gcggtgtgag cacgtactat gcgcatctgt
     6961 cccggatcga cgtaatcgat gggcaggaaa taaggcgagg cgaaaccgtc ggggcgctgg
     7021 gcgccactgg aagagtgacg gctccacatc tccattacga agtccgggtc ggaaataacc
     7081 cggtaaaccc atacagcttc ctggcgaagt cggtgttcgc gaagaacgcg acgaaggatt
     7141 ttcctttcta gattctggga atcattagcc cggcttggcc ggtaaaacca aggaggcccc
     7201 tatggacgcg gcgctggttc gtctgctcgc cgtctgttcc gcagcggttc tagtggcctg
     7261 ggggggccgc aagacgtccg tgattccagt gatgaccggt accgcggaga ctttccttgc
     7321 agctctcact cccgaccagc gcataaaagc cgctttggct ttcgagaacg aggaaaggtt
     7381 caactggcac tacatcccca gggcacgcaa agggatcccg cttcgagaga tgacatcgga
     7441 gcagaagcac ctcgcacacg cgctgctcaa tgcggggctg agccagcgcg gctacgtcaa
     7501 agccacgacc atcatgagtc tcgaggaagt gctgagggca gtcgagaacg acagcgggga
     7561 gcgccgcaac cctgacgggt acttcttcac tgtcttcggc cgtccgtccg agagcggcac
     7621 gtgggggtac cgtgtggagg ggcaccacct gagtctgaac tttacgattg ccggcggcag
     7681 catagccggg ggcccgacct ttttcggcgc gaatccagcc gaggtacgat cgggaccgcg
     7741 gaaggggctg agagcgctgt cgcgggagga ggatctcgcg cgggatttgc tggccagctt
     7801 ccgggaagag cagatcgcgg cggtgatcgt cgacaagacc gcctatggcg acatcctgac
     7861 tgcggcttcg cgtaaggcgg ctctcgcggg acaaccgagt ggactgccgg cggcgcggat
     7921 gacggtagcc caacgggcca aactggaggc gctgctgcgc gagtacgccg agaatctgcc
     7981 ggcggaactg gctgacgccc ggatggtgag gattcgccag gcgggaggag aaattcgctt
     8041 tgcctgggcc ggcggcacgg cgcgtggcga accgcactat taccggattc aggccccggc
     8101 ttttttggtg gagtacgaca atacccagaa caacgccaac catattcact cggtgtggcg
     8161 ggacttcgag ggggacttcg gattggactt gctcgaagcg cattaccgcg ccagccaccg
     8221 ctgaggacgc acgcggaccg cgtgctacga tggcagatat ggaaggcaag ggccgccggc
     8281 ctgccgggtg gacacgacga gccctgctca ctggacttgc aggagttagc ctgccggtgg
     8341 cggcggatgt tggccacccg tttccttcgg atcgggttcg tttcctggac cgtgccaccg
     8401 agttcgaagt ccttcggctg accgatccgt cgtactccag catcctgccc cactattacg
     8461 cgcgcgcttt tgcgaaaaag cgaaacgctc ttctgttcgc ctgcgaccgc ggcggatcga
     8521 tacagggctt ccagatggat ctcaaaaccg gagcatcggt gcaattgacc gaagctacgg
     8581 gcctagacgc cagctcgctg acctggctgc cggatgagcg cagttgctgc tatttcgacg
     8641 gcgtttcgct gcggcagttg aatgtcgcca accgccgttc ccgcgagatc taccgtgtcc
     8701 ctgacggctg gcggcgcggc tcaggtttca gtgtgtccgg tgacggcgtg cacgcgtttt
     8761 tcatggagat gcaggcggat acctgtcggc tgcgtatcgt cgggattgcg aaaggtgacg
     8821 cggtgacggt cgcggaatcc agtgaggtaa tgagcgaccc ggttccgagg ccccgccgat
     8881 ctggtgtact gtaccggcgg ggtggtaacg ctctctggct ggtcaattat gacggacagc
     8941 agaaccgtcc gctcaagacc gctcccggtg gcctcggccc ggtccactgg tcgcctgacg
     9001 ggaaatcgat catttacttg aattttccgg aagaccggcg cgatctgaac tcactgcgag
     9061 agcacacgcc ggattcaaat agcgacgaac tcatctcgaa gaccagccag ttcgtgcatt
     9121 tcggactgaa cgctgacgga tcggtgtttg tgggtacgag cgggagcaag gcttcgcctt
     9181 acctcctgct gatgctgagg gtcacgcacc gcgagttgac gctctgcgag caccgtgcct
     9241 cggacccgaa ccgggtgacg cctgtcttca cgccggacag ccaacaggtt gtgtttcaga
     9301 gtgaccgtga cggtaaactg gcgatctacg cggtgcgggt agaccgtcta gtggagaaaa
     9361 cagattctta gctggggttt cggacccccc tatttgagca acgatggtgc ggtggatagc
     9421 ttcggcggta gtggcctcga ttacgatacc gtgtacggcg gccaccaaga tcgtggtgac
     9481 ggtggttgag aagaggtcgg gccggcctgt gcccgagctg aaagccgacg actttattgt
     9541 gctcgacgat cgcgcgcccc ggcgagtgga gtcggccgaa tttgcgtccg gcctgatgga
     9601 cgtaatgctg ctgctcgata ccagtctgct cgggggggcg gttcagcccg tcgccgaaaa
     9661 tctggtcgcg cagttgcagc cgaaggagca gatggcgatc gtcgcctttc actcgtccgc
     9721 cgacctggtc caagacttca ccgccagcca ggaattgctc ggccgggcga tctcatcggt
     9781 gaagtacggc aacacccctc gcctgctgga cgcgatctac gccgccatcg acggagggtt
     9841 ccaggcgagc acattccgcc gtgtgatcgt gctgttgacg acaggggtcg agggcccgag
     9901 ccgggtcagc gagcgcgaag tactgcgcct ggcacggcgg aacggagtct caatttaccc
     9961 ggtctatgtt gtgggacagg aaagatcgat gtttgagaac ctggcccgcc agacgggtgg
    10021 cgcttcattc aatcttcgag acttgcagcg ggattcgaag tcgccgcccg ggcctcggat
    10081 cttcgaggtg atgcgatcgc actacacgct gacgctggaa ggaaacctct ccctcggcga
    10141 cagggtagaa gtaaaagtaa aaaggccgga gaagctgctt gtctccggcc ttcccctcga
    10201 ttgactgagc ggtgcgggcc cacctcagga tctcctcaac ccacactcca ctcgctggag
    10261 gtaagctact ttcttgcgat ctgaaccttg ggggtcgcca ggtagcccgt aatgcggtcc
    10321 ttctgcacct cattgaccac gatttcgtac ggaagccgag ctttggccac atagaactgc
    10381 acgggctcgt tggccgtttt gtccttcttc tcgactttct tatcgtcggc gaggacgtcg
    10441 atgctgtatt tgctgcgctt cggatcggtc ttcttcagga cgaccgatat gtcaccaatc
    10501 cgttgcggct gttttgactt cgcgagtttg aactcaaagt agttgcgctc cccgagcgtc
    10561 ctcagcgctg ccagttcctg accgttggtg gcgatcaacc cgctctggac tcccaagtca
    10621 ccccggacac ttttcagatc agcgatcgtc ttttccagtt cggacttggt agatgcgact
    10681 tcggtcttga cgttcgtcac ctcgccagaa acgtcagtga tcttgttatt ggccgtgctg
    10741 gctgcctctt tgacctcggt cagttccgtt ttgaccatct cctgctgccg ggcctgctct
    10801 gtctggatct gttttgccag ttgttccgca tgcttcagag cgtcggtccg ggcgcgaccc
    10861 gccgccatcg ccgcctgctt gcgggtgttc tccaattccg agcgcagctc atcgagatgc
    10921 tgctggttgg aggccgtgct aatggacgac gtctcgcgga ctttggcgag ctcagcgagg
    10981 acggattccc gcatgctgtg caggtcggtt ttcaacccgt ccaccgtgaa aaacagatac
    11041 gccgtcgcgg ctgccacggc gatcaccaat ccgaatagga tcgggatttg aatgttgccc
    11101 ccacttcggg aaggcgtgcg agtctcggtc agttcactca ttgtgggtct cacctctggc
    11161 cataagaacg caagcttgat gccaaacgga gtgaaggctt taaacgattg aaaaaaaata
    11221 acttagagct cggatggcgg aagagaactc tccggggagc aagtaggaat taccggctcc
    11281 ccgcaattgt tgccggaaag gggcgtcaac gcaccgtctt gactttggga gtggccaggt
    11341 aacccacgat ctggtccttc cgaacctcgt tcactacgaa ctcgtacggc tgtcgtgcgc
    11401 gcgccacgta aaactggacc ggttcgttta cgttcttgtc ctttttttcg accctcttgt
    11461 catcggcgag gacttcgacg gtgtagcggt tgcgctttgt gtccgctttc ctgagccgaa
    11521 ccgagatgtc gccaaccttt cggtattctt tgctccgggt gatgttgaac tcgaagtagt
    11581 tcctttcgcc aagagtccgc agcgcggaca gctccttcga attggtggcg atgagaccgc
    11641 tctggacgcc catgtcgccc cgcaccgtct ttaactcggc aatggttttg tccaactccg
    11701 atttggtcga agagacctcg gagcggacat tcgtcacgtc cttggacacg tcttcgatct
    11761 tcgtgttggc gtccgaggta gcctggcgca cctctgagat ctcactgcgg accgcctgct
    11821 gttgcttttg ctgctcctcg gcaagacgtt ttgcgagttc ctccgcatga cgctgcgcct
    11881 cggttttcgc ttgcccggcg gcgacggcgg cgttgcgacg tgccgcctca agctgttctc
    11941 gcaacgcatc gaggtggcgc cggtttgttt cgcttgtgac ggacgacgat tcgcgcaagt
    12001 tggttacctc gttcagaacg gactcccgca tctgggtaac ctccgtcctc atcttgtcga
    12061 gctgcacaaa gaggtagacg gtggctgcgg aaagcgcgat caccgctccg tataggatag
    12121 gcaagaggat cttcgaggtg cgaccggaag agccgtaaat ttcgtccgtt tccatagtgg
    12181 ttggattcct cccaactgga gagccgaata actacctttc acttcagtct actgcggatt
    12241 caggttactt ttccagccca ctcactgcct cggccgcgcg gcggaggcgc acaaactcga
    12301 gcacttcctg tttcgggaga cccggctcga aacagcggcg gcaacgtgct tcgtacatgc
    12361 cagcggctcc aacgacgatg aggtcatcgg aatctaccag ccgctgggtg tgcttggccg
    12421 ggttgccgca tcggacacag atagcgagtg tcttggtaat cgactcagca agcgcgagaa
    12481 gctccggaac gggcgtgaac ggtctcccca tatagtctgt gtcgagaccg gcgacgatca
    12541 cctgctttcc acggtccgct agacgcccgg caacctcgat gagtccaggt cccatgaagt
    12601 tggcctcgtc gactccgacc acttcggtac gccagtccat ccgcgagagg atttcggcgg
    12661 catccgggac gatctcggac ttcatgcgaa cctcggcgtg cgagacgatc tcgtcctccg
    12721 agaagcgggt gtccagagac ggcttgaaga cctgcactct cttccgggcg atcatggccc
    12781 ggcgcaggcg gcggatcaac tcttcgctct tgcccgagaa catgggcccg catatcacct
    12841 cgatccagcc gatgttcccc gagatgaagt ccatggggaa tatgatgaca cgccgtgttc
    12901 cgtgatgtgg tcctggctca agtcctgatg gatagacgga ttatgggcta ttctgccaac
    12961 ccaccgccgt gcggctataa tcgtcttgtg aattctcgcg ttctctgttt catggcgggc
    13021 atcgtactgc ttgccgggtg caatcgcggc gtcgaaaaca aagaagcagt gcggcaggct
    13081 gtcattgacc acctcgccaa ccgcgccggc ctcaacgttg cctccatgca ggtcgatgtt
    13141 tccgcagtgt cgtttcgcaa ggacgaagcc gacgctaccg tcgtcttccg ttccaagggc
    13201 ggcgctcccg gccagtcgat gaccatgaac tataccctgg agcggaaggg tggccgctgg
    13261 gtcgtcaagg gcagatctga aagcggcatg cctcacggag ccgcgggaac cccggccccc
    13321 ggcggacaga tgccgcaagg ccacccgccg atcggcggcg ctaagccttc cgaagtccct
    13381 aaatgaagcg cgtcgccatc ctcggtggtg gtcctgccgg tgcattcgcc gccgaacgcc
    13441 tcgcggcggg cggcgccaaa accgttgtct tcgacgagaa actcgcctgg gaaaaaccct
    13501 gcggcggcgg tctcacctac aaggcttata gccgctatcc cttcctcatc gaaaacgaca
    13561 caccgaagaa gttcgtcact gtgacccaac ttgcggcgcc cggcgccgga gttgcgaaga
    13621 tggcgttacg taagcccctt gttgtctact cccgtttcga cctcaaccgg atgctgctcg
    13681 cgcgggccga acgcgccggt gcacagatcg aacagacccg ggtcctcggc atgacgcccg
    13741 ccggaagggg ctggcggctc tccacgcacc acggtgactt cgaggctgac ttctgcgtcg
    13801 tcgccactgg cgcgcgcaac ccgcttcgcg acgtcggcac ccagctcacg gccgccgaca
    13861 ccatgcttgc cctcggctac tatgtccagg cggaccagga gcacatcgac atccagtttc
    13921 ttccgcaact ggagggctac atctgggtct tcccgcgttg cggccatctg tcggtcggca
    13981 tatgcggcaa aggagaaccg gcccatcaac tacgcgcccg cctagaggca tatatgcggg
    14041 agcgcggcat ctcctggaaa gacgcggtct tctatagtca cctgctgccg tcgctcgccc
    14101 ggacgggatg gcggaacaac cgtgtcgcag gccatggatg gatcgctgtc ggcgatgccg
    14161 gcggcctcgt cgacccgata accggcgagg gactttacta cgcgatgcgc tccggcgacc
    14221 tcgccagcca ggtcgtgctc tccggtcggc actctgccga gaccatggca gtcgcctacc
    14281 gtgccatgct gacccgcgac ttcgcagccg acctagagtt cggagcctcg ctcgcgcgcc
    14341 gcgtcttcct gggccgcttt ctgctggaca gcgtgccgaa ccgaatggtc caaatcatgc
    14401 gccgcagtcc ccgcttttgt gagctgatgc aagatctctt cgccggcacg cagccctatc
    14461 tcgacctcaa gcggagactc ctcagcaacc tgcatggaac cctgctcgag acggccatga
    14521 acttcctact cagccgcgtc gtcccgagcg aaacttagcc atgcgccgac acacctccga
    14581 atcccttttt gaccgcgccc agagatcgat tcccggcggg gtgaactctc cggtccgcgc
    14641 cttccgcagc gtgggaggca agccactgtt catttcccgc ggggagggct cgcacatctt
    14701 tgacgcggat ggcaacgagt atgtcgacta cgtcggttct tggggtccgc tgctgcttgg
    14761 acaccgccac cccgctatcc ttaccgcgct cgaagaggcg gcgcacagcg gtaccagctt
    14821 cggcgcgccc accgaacgcg aaatcgaact tgcggaagct atccgcgacg ccgtcccctc
    14881 catcgagatg gtccggctcg tcaacagcgg caccgaagcc accatgagcg ccatccgtct
    14941 cgcccgtggc ttaacgggcc gcgacttgac cgtcaagttt gagggctgct accacgggca
    15001 cgtcgactcc ttgctcgtga aggccggttc cggcgtggcg acgctcgggc ttccggacac
    15061 cgccggcgtt ccgaaaagct tcgccgacac taccatcgcg ctgccgttca actcggccga
    15121 ggccgtcgag caggccttcc gcgaacacgg cgcgcgcatc gcttccgtga tcgttgagcc
    15181 ggttgccggc aacatgggct gcgtgccgcc gaagccgggc ttcctcgcgg ctcttcgtga
    15241 gatcaccgaa cggtacggcg cactgcttat cttcgacgag gtgatgacgg gttttcgcgt
    15301 ggcttggggc ggcgcgcagc agctttatgg aatccgtcca gacctgacca cgctcggcaa
    15361 agtcatcggc ggaggccttc cgatagcagc ctatggcggg cgcgcggaag ttatggcaaa
    15421 gatcgcgccg ctcggtccgg tctaccaggc tggcacgctc tccggcaacc cgatggccgt
    15481 ggcagccggt ctcgccatgc tgcgccacct caaggcccac ccagaggtct acaccacttt
    15541 ggagcggaac ggcgagcgat tgcaggcggc agcgccctcc gaaatcaccg ttaaccgcgt
    15601 tggctcgatg ttcacgttct tctttaccga tcagcgggtc acggactacg actcagccaa
    15661 gacctgcgac accgcgcgat tcggcgacta cttccgcttc atgctcgacc gcggcgtcta
    15721 cctcgcaccc tcgcaattcg aggccgcatt cctctctgcg gtccacacgg atgaagacat
    15781 cacacgtacg atcgaagccg ccagggagtt cttcgctcgg cggtagcgaa cacagtcagc
    15841 gcttctccgc gtaaaccgtt gcgcctccca acaccgtcat cagcacccgc gtctctagga
    15901 tctccggtgg cgctgcgcgc atgatgtcgt ttgacagcat cacgaaatcc gccatctttc
    15961 ccggtgccag tgatcctttc tctttttctt cgaacgctgc gtaggcgcat gtcaccgtcc
    16021 agctcttgag cgcctcctcg cgcgtcattc gctggtccgg gaaccatccg tccttcggat
    16081 ttccatcgcg gtcctgacgc gtgatggcgg cgtaaaaccc ccacagcggg ttcggctcct
    16141 cgacggggaa gtcggaaccg ttggccaccg gcacgccgat cgaaagaaac cgccgccaag
    16201 cgtagcaccc cttcacgcgc tccggcccca cccgcgcctc ggcccaccgc atgtcgctcg
    16261 tggcgtgcgt cgcctgcatc gaggcgatca ctgagtattt gccgaacagt tcgaagtccc
    16321 ccggcgccac gcactgcgcg tgttcgatcc ggaatcgctt gtcgttcttg cttccgagca
    16381 cggcccggta tgcctccagc acatcgtggt tcgcccggtc gccgatcgca tgggtgttca
    16441 cctggaatcc cttcccggcc gcctgccgcg ccacgcgctc cacgtcctcg cgggagaaca
    16501 tcaccagccc tttgttacca ggatcgtccg agtaggcttc gaagaacgcc gcgccacgcg
    16561 agcccatcgc cccgtccgcc accaacttga tgctgcgaac cgttagcctg tcgccggtct
    16621 cgggtccgcg ctcaaggtat tcgcgccaca gcgggccctc gccgccgatc atggcgtaca
    16681 cgcgaactgg caaccgccct tcgcggatga gcgcccggta cgcagccagt tccctcgcgc
    16741 ccacaccggc gtcatgaacg ctggtgatcc ccagccgcgc gcattcccgg gctgcacgct
    16801 cgagtctgcg gcgaacctgc tcgtcggtcg gattcggaat cttccgctcc accaggcctt
    16861 gcgccgtgtc cacaaggatt ccagtcggct tgccattcgc gccgcgcacg atcttgccgc
    16921 ccggcgggtc ggctgttttc gcggtaatgc cagcgatctc caaggcctta cggttcaccc
    16981 aagacgcatg cccgtccacg cgcgtcaaaa agacaggatg atccggtgcc gctcgcgaaa
    17041 gaggttcctc ggtgggaaag cgcccgcccc agttggtttg gtcccagttg cgtccctgaa
    17101 tccactctcc ccgagtccgt ccgctggcgg ccttgcgcac gatttccgct acctcctcga
    17161 ccgtgcgcac ataccggaag tcgggattct ccaggctctc ccccagcgcc agcatgtgga
    17221 cgtgcgaatc gatgaggccg ggaatgatgg acgcgccgcc ggcgtcgatc cggcgtgtac
    17281 tggacccggc gaggcgcgaa agattgtccc cgatcgcaag aattcgcccg cctttgacgg
    17341 cgatggccga agccaccgga tttcccggct cggcggtgta aatccgtgcc ccttctatca
    17401 gccaatcggc ggtctcggtg ggtgccatga ctgctgccaa tgagactaga ataaggaaca
    17461 tgcacgacag tatggcacac gccgtggacg agtctgcccc tttagaatgg cctgcgtgga
    17521 agacgtgggt cagcgcgatt tcggcggtgc ttctggcgat cctgttcatc gtttccggcg
    17581 tctggaaggt caccgatccg cttgcggccg cggtgagaat ggcgcaggcg aaagtcccga
    17641 atgtgctcag tcttccgact gcgattggct tcggcatcgc cgaagcgttc gccggtgtct
    17701 tgctccttgt gccccgcttt cgccgttggg gcgcgtggct gaccggcctc atgctggtgg
    17761 cgtttctgat ctacatcggc gtcctctaca acgacctgcg aggagaagac tgcaactgct
    17821 tcccctgggt caagcgcgcc gtcggtcccg ctttcttcat cggcgacgcg attatgctcg
    17881 cgatggcggt cctggcagga tggtgggtaa ggccttcaca tggccttcgt agcgcatcga
    17941 tcatatttgg cgcggtctgt gtcttcgcgc tagtctcgct cggaattcac ctgacgcgcc
    18001 aatccggcgt tctggcgcca gaaacggtca cggtcgacgg gaaggctttc cccctgcgtg
    18061 agggccgagt gttcctcttc ttctttaacc ccgaatgctc ccactgcaac cattccgcca
    18121 gcgagttggc gaagctggac tggggcgaaa ccaagattgt tggcgtcgtg acgcaactgg
    18181 cccagtttgg gcctgatttc atgcgggaaa ccggcctgaa aggcgtcgtc accgccgatg
    18241 tcggcttgct caagaatgcg ttctcgtttg tggacgtgcc gttcggcgtg gctctagaga
    18301 acggtcgcca gcgggcatct ttccccaacg tcgaccccga gccagccgct gagactctcc
    18361 gcaagcttgg gtttgtgaac tgacacggcg cgcaaagtaa aaacgcccct gacccactct
    18421 cgggccacgg gcgttttcgg ttttgcagaa ggatctagcc ccgccgcttt agcgtcgggc
    18481 gctcgtccgg gtcttctttc tgctggtcct tgtccttccc cttggacttg tcatcctcga
    18541 tcggcgcggt gctcccgccc ggcgccttgc ggagcgtcgg ccggttcaga tccttgtcgt
    18601 ccaccttgcg gcggttgtgc attgcggaaa gccgcgtctt cacttcgttg aattccgagg
    18661 tgttgacgac gtattccggc ttcgccttca ggatctcctg gatgttcttc tgcgcggtct
    18721 tgatccggtc gtccgtcatc ggatgggtcg agaaaacttt ggacatcgtc cctggcttct
    18781 tcttttcgag cgtttgaatc ttctcgaaga agtccacgaa tgccgtcgga tcataaccgg
    18841 tcttgtagag atactgcaag ccgagcaggt ccgcctcttc ctcgaagccg cgggagaacg
    18901 tcaggaaggt gatcgggatc gccaggccgg ctccttgccg gatggcgtag cccgtccatc
    18961 cacccatgaa gatcagggga atggaagcga tgttcatgag ctggccgcgg gaggcctgtc
    19021 gcgtgccgtg ccgggccgcc acgtgcgcga tctcgtgtgc catcacgccc gccagctccg
    19081 actcgctctc ggcacggagg attagtcctg aattcacgaa gaagaaaccg ccaggcagtg
    19141 cgaacgcgtt gacttcgtcg ctgtcgagca ccttgatcgt gaatggaacc tttgcatcag
    19201 agttccgcac aaggttctgg ccgacccggt tgacgtactc cccgatcacg ggatcatcca
    19261 ctaccttcgc ctgctgctcg acctcttggg cgagctgttt gccgagcgcg atctctttct
    19321 cgagcgagta aaagtttaca cccctgccga cgtcccggtt gccgatctca tccgggtctt
    19381 tcttcttgtc ttttgcgatt gcagcggtgg gaatcagcac acacgccgcc acgaggatgg
    19441 ccagcgccgt tttgagcttg cccgtcatcg cgatcctcct tggtcccaga gtgctagacc
    19501 tattatagta ccccccgctg gggagggacc ccggtcccac acttcattta acgtatattt
    19561 gtcttgatgg gttacgaaaa agcaacggaa tccgaagagg ttccactttg gaagcgcttc
    19621 cggcattgct tcccggtcac ggaaaggttg gtctacctga atcatgccgg agtcgcacct
    19681 atatgcaagc cagccgccga cgctatgtca cggcttacgg aggatgctct tcaattcggt
    19741 tcctaccact acgattcgtg gttggccacc tacgaagggc tgcgggcgat cgcatcccgc
    19801 ctgatcggag ccagccggga cgaaatcgca atcgtcaaga atacgtcgga gggaatcgcc
    19861 acgttggctt caggtctgaa gtggcggcag ggcgacaaaa tcgtcgcctt taccgaggag
    19921 ttctctgcta actacttccc ttggaaacgg ttggaggcgc agggtgttac cgttgagtgg
    19981 ctctctgtgt tcgacacgtt cgacaaaatc gaccgggcgt gcaagggcgc gcgtctgctc
    20041 gccgtcagct acgttcagta cctcagtggg catcgcatca acctggaagt gctcggcgag
    20101 atctgcaacc tgagaggctg tctgttcttc gtcgatgcca tccagggcct cggtgcgttc
    20161 ccgcttgacg tccgcagttc gcggatccac gctctgtcgg cggacggaca caagtggctg
    20221 ctgggcccgg aagggtgcgg catcctttac attcacaaga acttattcga ctccgttgag
    20281 ccgcccatga tcggctggac gaatttcgca cattccagcg actatgagtc gcgtgacatg
    20341 accctacgta ccgatgccgg gcggttcgag tgcgggacac ttaacacagt gggctgcttc
    20401 ggcctgcggg cctcgatcga attcctgctc tcggtcggcg tcccaaaaat cgccgtcgcc
    20461 gtgcagttgc tgggcgatca gattgtccag ggggtcaccg ccaagggcta tcaggttctt
    20521 ggccgcagga ccgcgagcaa tggcgccgga atcgtctgtt tccgccgttc agacgtagac
    20581 agccgtctga tcgtgcggga cttgaaagcg aaagggatcg ttgccgcgag ccgccagggg
    20641 tgggttcggg tgtcaccgca cttctacatc gctccggacg atatcgatcg cttggttgaa
    20701 gaacttccgt gagggcttag gcgaccgacg ccgacgccag ctcctggatc cgtgccaccg
    20761 ttctcggcac tgccgccagc acagccggat gcattgcgcc gcccacggtc aagcagtcgt
    20821 gaggctccac cgccaggaat aacacctccg gcggaacctc caggccaagc ttccggccga
    20881 gagccaggac ctgaaacagc ccgatgccgt gtggaaactc gcccggtaga ggcgaaatat
    20941 cgtcctcccg aaactcgtac agcgttcccg gcggcgcctt acgtgtttcg atgacgtcaa
    21001 ccaccgccag cgatgcggcg cagagaatgt cgtcgagaag atggaagccg gacgcctccg
    21061 acgtcacgac ctccaccccc tcgatcgaca actcgttcag acgcccggcg acgcgaatcg
    21121 cgaacgcatc gtcgccgagg atctcgtttc ccaggcaaag aactctcagc cttggaggtg
    21181 cttcgcctgc catggagtgg ctcagtctct cctcacgctt ccgtcgttgt cgcgccgaag
    21241 cgtcgccgtc gtggctccgt tcgcgtcgcg cacccggaca aacagcggca tgctgcccgg
    21301 caacgagtgc gtcgcgcagc ccaggcaggg atcgtaggct cggaacgcca tctcgaccat
    21361 gttcagcacc ccgtcggaga tcttgtcgcg ggtaacgaaa cctttcgcgg ccttctccac
    21421 gctcatcgcc attcgcgctg cgttgtgctg ggtggccaca atcaggttcg ctttgcggat
    21481 caggcccttc tcgtcggtct ggtagtgatg gatcagcgtt ccacgaggcg cctcgatcac
    21541 gccgattcct tctttcggtg tcgccgtcgg cagtttgcgc acgtttggat cggtgagctc
    21601 cggatcttcc gccagttcct tcatccgctc cgctgcacac aacatctcga tcatccgcgc
    21661 ccaatggagt gccagcgtat ggtggaccgg cttgcctccg agcgtggcaa agaactgctc
    21721 gtaggccgcc tgcgccagcg gcgtcggcat tccatccgac acgttcaatc gcgccagcgg
    21781 cgccacgctg tagaccccgc tgtccgctcc gtccacgaag cccttccacc cgatcggctt
    21841 caggtagcag aacttcatat agctccacgg ttcgacatgc tcggccacgt agtcgagata
    21901 ttgattctgc gggaacttca tcaactcttt gccttcgggc gaaaccacgc gcagcttgcc
    21961 gtcgtaaaag ttgacacggt tccgctcgtc caccgtgccc atgtagtggg tgcggtgagt
    22021 gtaggtgtcc gagacgatca ggttgacgta gtccgagttc ttcagcacca cctggtcgaa
    22081 caggtccagc gtgaataggc agaaatccac cgactcgccg gccacccgcc ggaactgctc
    22141 ctgatcatcc ttcgtgatcc gcttggccac accgccggga agcccgagaa cggggtggat
    22201 cgccttaccg cccaccagcg tcatcaggtc gcgcagcttc cggcggaagc cgatgacctt
    22261 cttgcccgcc tccagcccga ccttggctac cacgccgagc acattccgct cggcagccgg
    22321 tgcgttcggc cctaccacga agtccggccc accgaggaag tagaaatgca gtgcatggtc
    22381 ctcggccatg aaggtgttgt agaagagttc gcggatcttc ttgcctgccg gcggcggttc
    22441 gacctggtag aggtcgtcta gcgccttcgt cgccgccatg tggtgcgccg acgggcagac
    22501 accgcagatg cggctggtga tctggggcat atcctcggcc ggcctgcctt cggcgaactt
    22561 ctcgaagccg cgcagctccg gtacctggaa atacgcgcgt tccacctgac cggcgtcatc
    22621 cagaaagata tcaatcttgc catggccttc caggcgcgta atcggatcga tggtgacccg
    22681 gcgtgttgga gtagtttcaa ccgacatgag ctatgcctcc aatgtcttcc gtttcagcag
    22741 agatgccggc aggctgtacc ggtagaacgt accgacggga tcgggaatcc ctgccagaac
    22801 cttctcgata tcggtatcgt ccttggcctc gatgatcgac gccagcgccg tcagggcctt
    22861 gccgccgtaa tcgcgaaccc ggctcgtcgg cccgtagcag cccgtgcacg gcatgttgcc
    22921 cttcgtgcag agccccccgc agcctccgcg tgttgccggt cccaggcaga gcaacccttg
    22981 agcgagcagg cacttttccg ggtcggcgat gatctggtgc ggccgcttga actccgcgag
    23041 ttccagcttg tcgggacgcg tgtcccgccg cgggcactca tcgcaaagcg cgcggtccgg
    23101 cgccagcacg gtgccctttg gcggcagatt cccgctcagc aacgtctcga cggcgtccag
    23161 taccaacttc gccgccggcg cgcaccctgg gatgtagtag tcgacatcca ccacctggtc
    23221 gagcgtccgg accgtgttat aaagcatcgg cagcgtaggc gttctgccgt tgtctttgta
    23281 tgcggtctgc ggtgtcgtgc cctcgggatt caccgtgctc ggcgacccct tgtagaccgc
    23341 ctcgagaatc gccgaacgat cccacaggtt cgcgagtccc gggatgccgc ccaactgcga
    23401 gcaggagccc agcgcgacca ttaccttgga cttccgccgc agcaggtgtg ccatctcctc
    23461 ctgctccgtg ctccgtatcg cgccgttgag gaacgtcgcc acgatcccac cgtccggtat
    23521 cgcttcgatg tccttgcgct tgaagtccaa cgccaccggc cacagcacga tctcgaccgc
    23581 cgccgacact tccaggatct tctcggcgag atcgaccacg gcctcctcgc aaccgccgca
    23641 ggaggcgcac cagtagaatg caacttttgc cttctcactc atgacgcagc tacctcctcc
    23701 aactctggct cgatggcggg gttcatctta cgtggcccga gcgcccgcaa cttctgaacc
    23761 atgtcgttga ccacggtccg cacccggtcg ccctccgcgg cggagatcca ctcgaggcga
    23821 aaacgctcct cctcaatgcc catctcgcgc agcgtgcgct tcagcaggta ataccgccgt
    23881 agcgctttat agttgccctc ctggtagtgg cagtcacccg ggtggcaccc gccgatcagc
    23941 acgccgtcag cgcccttcgc gaacgcttcg agcacaaact gcgggtccac gcggcccgaa
    24001 cacatgacgc gaatcacgcg cgtgttcgcg gcgtacttca tgcgagacgt tcccgccagg
    24061 tccgccgcca ggtaggtaca ccagttacag aagaaggcga cgattttcgg ttcgtaattc
    24121 tcaggcatag gtcagaactc cttcgatctc ttcaaagatc tggtcgtcct cgaacaaatg
    24181 ctgtttgatg gatcctgaag gacacgcggc gacgcacgtg ccgcagccct tgcagagtac
    24241 ttgatgaatg ccggctcgct ttgtgtcggc gttgaaataa acggcgctgt acgggcacat
    24301 cggaacgcac gtcttgcagc ccgaacactc gtcttcaatc acgtaggcag tgttcggttc
    24361 ctgctctacg aatcccgtat cgatcagcgc cagcgcctcc gccgccgctg ctccggcctg
    24421 cgccaccgtg tcgggaatgt ccttcggtcc ctggcagcag ccggccacga aaatgccgtc
    24481 ggtaaacgtg ctgaccggcg ccagcttcgg gtgccgctcg aggaagaatc cctcggaccc
    24541 gcatccgaaa ttgaacagcc ggcgaacgtc ctgggcgtcc tcatggggct cgatccccac
    24601 cgacagcacc accatgtcca ccggaatccg gcgcactgtg ccaagcagag tatcctcgac
    24661 acggagaacc agcttgttct cctctgtcgg gtcgagcgcc cagtcggtaa cttcggccac
    24721 gcgcccgcgg atgaagtgta cgccctcgct caacagccgc tggtagaatt cttcaaaccc
    24781 ttttccgggc gtccggatgt cgatatagaa gttgaaaatc tccgcatccg tgtgctcctt
    24841 gagcaggtgc gccagcttca gcgagtacat gcagcagacg cgcgagcacc accggttcgt
    24901 cccttcgtcc cgcgacccca cgcagtggat gatgccgacc gtcttcggtt tccggccgtc
    24961 gcgaagcacg acgtcgcccg ccgtcggccc cgacgcgttc accagccgct cgacttcgag
    25021 cgacgtgtac acgttcggat acgtgccgta cccgtagtac ggtgtccgcc gcgcgtcgaa
    25081 cgctttgaag ccggtcgcca tgataatcgt cccgacttcg atgttgcgta tctcttcttt
    25141 ctgcgagaag tcgatcgcat gcctgtctcc gcaggcttgt acacaagtct tcttgcactt
    25201 gccagacttg aactggatgc acgtgtcgat gtcgatcagc ggcatctgcg ggacggcttg
    25261 cggaaacggt atgtagatgg gcttgcgttt gccgagtccc atgttgaatt cgtcgatgaa
    25321 cttcggctcc ttgtagacgc aggcttcgat acactccagg caccccgaac agaggtcctc
    25381 gttgatgtac cggggccggc gagcgacttc gaccttgtag ttcccgacgt atccttcaac
    25441 cttcgtcacg tccgagtggg cccacaacgt gatgttcgga tgcgccttca ccgacgacat
    25501 cttcggcgtc aagatgcacg ccgcgcagtc gagcgtcggg aatgtcttgt cgaacatcgc
    25561 catgtggccg ccgatcgttg gctggcgctc caccaggtac acctttttcc cggcgttcgc
    25621 cagggtcagc gccgcgtgga tgccggcgat tccgccgcct accaccagca cgtcaggatg
    25681 caccggcact cgaaccttct cgagcgactg gtgaaacaca acccggcgca ccgctgcccg
    25741 taccatgtcc tgggccttct ttgtcgccgc ttcccggttc tcgtgtaccc acgagttgtg
    25801 ctcgcggata ttcaccatgt gcacgtagaa cgcattgaga cctgcgcctt ccgccgccgt
    25861 gcggaacgtg tgctcgtgca ggtgcggcga gcaggacgcc accacgatcc ggttcagctt
    25921 gtggtcccgg atgtcctgcc ggatcagttc ctgccccgga tcagagcaca ggtacttgta
    25981 gtccctggat accgttacgt gatacaggcc tccggcgtac tcggccacgg ccttgcagtc
    26041 cacaattgcc gcgatgttcg ttccgcagtg gcagacgtag actccgattc gaactatgcc
    26101 gtttcgctca gacatacgcc tccacctcct catgggccgg gaaccatcgc ttgatcaggt
    26161 cgcggccgga aattcccctg tgcagcccga gtttcttcgg gtcgccgccg agcgcgtagc
    26221 ccagcacttg ggtgaagtag aggatcggca tgtcgatcgg ttcgttggtt gccttgcgga
    26281 tttccgcctg gtacgcgtcc aggttgaact ggcagagcgg gcagatcgta acaatcgcct
    26341 cggcgccctt ccgggccgcc tctttgagca ggatgtagtt tagccggacg cccacttcgt
    26401 ggaccgtgcc ggtcagcgag cctccgcagc acttcgtctt cagcgcgtag tcgacggtcg
    26461 gcgccccgac gtcctgcagc agttcgtcca tccgcatcgg gttgtaggcc gtgtccacct
    26521 cgtcgtacgg ccgtaccgcc tggcacccgt agtagcaggc gatcctgccg ccccgccacc
    26581 gccggatgac attcgacttc agggtgtcgc ggccgacgtc gttgtacagc atctcgagcg
    26641 gatgccggac cttgacgcca tcaaacggtg gcagtcctcc cctggccagc gactcttcca
    26701 cttgcgtgcg gatcttcgga tgtttggctg cgtagtcctg agtcttcttc agcacgaggt
    26761 agcacgcggc gcagggtgcc agcaggtcgc gtgagttcac cgtgtgcgcc agcgaaaggt
    26821 tgcgggccga gagcaggaac gccgaaccct cgtcaatgca catgtaggat gtcgcgccgc
    26881 agcaattcca gtccggcagt tccaccagtg gtatgttcaa cagccggaac aattccacca
    26941 ggctctcctc gtacgccacc ccggttgcgc ggagcgagca gccgggaaag tagaggtatc
    27001 ctttcgatgt cttcgcggcc tgttccggcg tcatcgggac acctcctgag gatcggcgag
    27061 ctcgttctga agctctcgaa ttctggtgat ccgctcgctc ttcagggaca tccgtcctgt
    27121 gcgcagcaga ccccagcccg tcttcgccat cgtcagcagc acgagtggat tcgacttcat
    27181 agccatccgc agcacgagcc agaactccga gttgcggccg cgccggcgca cgatctcata
    27241 gaattcacgc gccagaacgg gaatgggaaa ccgggaagga tacagcttgt gcttgatcgc
    27301 ctcacgcttg aggctgtaca tcacgtcggt gatatgaacg ttctggggac agtggaccgt
    27361 acacgagtag cacgacgcgc acaaccagat cgtctggcaa ctcagtgcgt cggtccggaa
    27421 tccctcccgc accagggcga tgatgcggcg aggagtgaaa tccatgtata tagatagcgg
    27481 acaagtgccc gagcaggtcc cgcactgtat gcaggaaaaa acccgatcgc atccgggcgc
    27541 cttgctgatc tgttccgccc actccggatc agcttcccgc tgaaacttca tcttccttcg
    27601 gatgagtgca agcattgctt atctcgtttc agctccggaa ttgtcaaaca tcgcggcagg
    27661 aatacagcca ctctcagtat ataacagaca atcaggtctt aatatagctt gcttgtccgc
    27721 ggccactcgc tcacacatat gccatcacgc cgcgaatctc ttcaaagatc tcgtcgtcct
    27781 cgaacaggtt ctgcccgatc gagccggacg ggcacgccgc cacgcacgtg ccacaacctt
    27841 tgcacagcac ctcgttgatg tgcgcttttt tcttctcctc gagaaaacgg atcgcgctgt
    27901 atggacacag gcccacgcag gttttgcacc ccgagcaatc ctcctccagc acgtaggcag
    27961 tgttcggctc cacttcaatg aaaccggcgt cgatcagcga aaacgcttcg gccgcggccg
    28021 ctcccgcctg cgccacggtg tcgggaatgt ccttcggacc ctgacagcat cccgccagga
    28081 acacgccgtc ggtgaaagta ttcaccgggg ccagcttcgg gtgacgctcg aggaagaagc
    28141 cctcattggc gcacgtgatg ttgaaccgcc tcctcacctc ctgcgcgtcc ggctgcgcct
    28201 ccaatcccac cgccagcacc accatgtcga cggggatctt tcggacctgc ccgagcagcg
    28261 tgtcctccgc ttgcatgatc aggcggccgg cgccgttttc ttccgggtac acgtcgccca
    28321 ccttgccccg gatgaactgc acgccttcct cggctacacg gttgtagaac tcctcgaacg
    28381 ccttgccggg cgtccgcata tcgatataga agttatagat ctgggccccg gtgtgttcct
    28441 tgatcaggtg cgccagtttc agagagtaca tgcagcagac gcgcgaacag taacggttcg
    28501 tgtgctcgtc ccggctgccg atgcaatgaa taatcccgac cgattgcggc cgccgcccgt
    28561 cccgcaggat gacctcacct ccggtcggcc cggaagcgtt caccaggcgc tccacttcga
    28621 gcgctgtgta cacgttcggg taggttccgt aaccgtagta cggaatccgc ttcgcgtcga
    28681 aggtctggaa ccccgtcgcc aggatgatgg cgcccacttc gatagtctcc agccgctctt
    28741 tttgctggaa gtcgatgccg ttcctttcgc cgcaggcctc aatacacgtc ttcttgcact
    28801 tcccgctctt gaactcgatg caggtatccg ggtcgatgac cacgacttgc ggcaccgctt
    28861 gcgggaatgg aatgtagact ggcttccgct tgctcagccc ctgattgaac tcgtcctcga
    28921 atctcggctg cttgtacacg caagcgtcga tacactccat acagcccacg cacagttctt
    28981 cgatcacgta gcgcggcttc cgccggaccg tcaccttgta gttgccgacg tacccctcga
    29041 ccgcggctac ctcggatagc gtccacagcg tgatcttcgg gtgcgaccgc accgccgtca
    29101 tcttcggtgt cagaatgcag gcggcgcagt cgagcgttgg gaacgtcttg tcgaacatcg
    29161 ccatatgccc gccaatcgtg ggctcccgtt ccaccaggta cacccgcttt ccagcgttgg
    29221 ccagcgtgag cgccgcgtga atccccgcga tcccgccgcc gacgaccagg acatccggat
    29281 ggatgggcac ttgccggcgc tccagcggcc tgtggagcgc gacgcgcgcc gcggctgcgc
    29341 ggaccatgtc cttcgctttc tcggtcgctg ccaatttgtc gtcgtgtacc caggaggcgt
    29401 gctcgcgcac gttcaccatc tggaagaagt acgggttgat ccctccctca gccgttgctt
    29461 ttcggaaggt cttctcatga aggcatggcg aacaggccgc taccacgatg cgcgtgagcc
    29521 cgtgatcgcg tatgtcgttc ttgattagtt cctgccccgg gtcggagcac atgtacttgt
    29581 actcccgcgc cactgtcacg ccgggaagcg tgcccgcgta ccgcgccagt tcgtacacgt
    29641 caatcgtgcc tgcgatgttt gttccgcagt ggcacacgta tgctccgatt cgtggagatt
    29701 ctgcgtcgat cattgggccg tgcactcctc taccagctcg atcagatccc tcaccgccag
    29761 cctgccttcc agtcctgtgg tcttcaccgc gtcctggaac atcaccaagt ccttcggaca
    29821 ggccaccgcc agtgtgctca cgccgtctgt cgttgcggcc tcgcggacgc ggttctccgc
    29881 cggccgctcc ttcacgcccg gcgtgtcttc catccagatc cttccaccgc ccgcaccaca
    29941 gcaatgcgcc ctcgctcgat tcctcggcat ctctacgagc ttcgcgccga gcgtgctcaa
    30001 aacccggcgc ggtgcgtcgt agatcccgtt gtaccggccg agatagcacg gatcgtggta
    30061 cgtcaccgct gcttcaagcc gccgctccag cggcaatttg ccggacttca acagctcgtc
    30121 gatcagctcc gtgtagtgca acaccgctcc attgccctcg ccgtactcgt tctttagcgc
    30181 gtggtacgtg tgcgggtccg tggtcacgat ccgctcgaac ttcgccttgc cgagcgcctt
    30241 gaggttcttg tcgcggagca tctcgaacaa cccctcttca cccacgcgcc gcgcgtcgtt
    30301 gcccgagttt tgctcctcct cgtagagaat gccgaaatcg accccgcctt tccggaacag
    30361 ccgtgcggcc gaccgcgtca ctggctgaag ccgtggatcg taggaggcgt agtcgccggt
    30421 gaaccacagg tactccaccg gttccttgcg cgcgtctttc ggcttgaagt cgagtccctg
    30481 gatccacttt gcccgcgccc gtggcgaggt gccgaacgag tttccgtagc gcgtgaagtt
    30541 catcagcgcc tcctggactc tcggctcgac ctcgcctttc gacaccaggt gccggcgcat
    30601 ctcgacgatc accggaatgt gctcgttgaa caccggacag cgctccatgc acgagtagca
    30661 ggtcgcgcac gcccagatct cctccgcctt caccacgccg cctgcgaaat cgccgttccc
    30721 gccgccgttc cgcaccagtt ccttcacgtg atgcatcagc atcttcggag agagcggcaa
    30781 tcccgcgtcg aaacacggac aggagcggtc acatcggccg cactccgcgc acgccagccc
    30841 gttaaacagc tcccgccacg tgaactcttc ccgccgcgac gcgccttcgg acgctgccgg
    30901 tatgcctcct tctcggagag ttccgaagaa gaccccgaag ggcgatgcca gcaggtgcat
    30961 gtgcttcgag tacggcagat aggccaggaa cccgagcacc gtcgccatgt gcagccacca
    31021 catcgcggcg tagatttgcg gactttcggt catcgacccc gcaagcgatg tgatcagtac
    31081 cacagcgatc agcgtcagaa tcagcgatgc gtctttcgat cgctccagcc tcggcggcgt
    31141 gaacacgtac cgccggaccg ccgcgacgat cagcgccacc aggcccagca cgccgaacag
    31201 ttcgagcacc gcagccatcc acctcacgct gtcggtgtag ggaatgggca ggaacggaag
    31261 cagcccgcgg atcaggttcc agaagaacga gctcgcatag aatacgaaag accagaagat
    31321 caggaaatga gcggctccga ttgcccgctc gccgaacatc ttcttctggc cgagcacgta
    31381 gatcgcgaac atccgcatgc gccgccacgg ctcgttccaa cgcacctccg gacgcgcctt
    31441 ccggagaacg ccgatgtacc gctctacgcg gcgtccgaag agaaagaacg agaccagcgt
    31501 cagggcccac aataccgcgt agcccggaac gccgaagtag accgacgtcg ccggatccgc
    31561 catccgccct tatcctttcc gcgcccgtac ggcttccgtc agcgccggca gcacgtccat
    31621 ggcgtccccg acgatgccgt aatgggccac gttgaaaatc ggcgcctgcg gatcgctgtt
    31681 gattgcgacg atcagcgagg cgttcttcat cccttcgaca tgctcgggtg ctccgctaac
    31741 tcccgcagcc acgtacagcc gcggtttcac cgacagcccc gacttgccca cctgccgcga
    31801 gagcggcaac catccctggt cgatcaccgg gcgcgatccg cagatagctc cgccaagcgt
    31861 ctccgccagt tcctccgcca gtgcgacgtt gtccttgctt tgaatgccgc ggccgactgc
    31921 gaccagcgcg tcttgctgtg aaatgtcgat gtcgccggcc tccggctcca ggtagcgttt
    31981 caggcgtacg cgcggctccg gcagtgtgac ggtcacgtct tcgactgcgg gcgcttgcgt
    32041 ggcccggcct ttctccggcg accgcgcgcc cggccagatc ccgaggatcg ccggttcgcc
    32101 atgcgccgtg acggtcgctt ccatcttgcc gccgtacaaa acacactgcg cctcgatccg
    32161 gccatcggcc accttaagat cagtgcagaa gttgatgccc ggcgcgctca gcttcgccgc
    32221 ggccagcgtc ccgatgccca tgctcacgtt cgtcagcgcc actaggatgg cccgcggcct
    32281 ccgttcctgc gccagttgca ctagcgcttc ggcgcagacg tccgggatcg gctccgcgag
    32341 cgacgagtgg tctgcataca acaccacgtc cgctttccca agcgcgcccg ccgactcctt
    32401 tcggccctca gccatcagca ctgcttcgag gggcacaccc agttgatcgg caacctcacg
    32461 gccgagcgcc agaagctcaa aactgacttc ggaaatctct cctcgccact gctctgccac
    32521 aacccatatg tttccggcca tctcgctctc ctctctacag cacgccccgc tcggcgaata
    32581 tcccgcagag ccgtccggca atgtcctcag gactcccttc gatcatctcc gccgcgcccg
    32641 tgaccattgg cttgttcatc gccacaagct ccgccagcgg tcccccggcg tctgccgcgg
    32701 tcagcgcccc cgctgtctcg atcggctgcg acttcatcgc cgcccgtact tttgccaccg
    32761 gcacgtagcg cggcggcttc tccgccgatt ggattccgag caccaccggc aggggcgtct
    32821 cgaactcgcc ccggacgccg cccgggtact ctttgatcac cgtcgcggtc ttcgctgcct
    32881 cgtcggcccg cacttgcgtc acaatcccca cgtatggcag ctccaggctg tgcgccagca
    32941 gcggcgcaat caacccgtcg aggtcgtcga tcgcctgcga gccggtgaga atcaggtccg
    33001 ctggcaggag tccggtagac tctctgagga cgcccgaaag cgctgccgcg gccatccgcg
    33061 tcgttgtacc cggttcaatg tcggcgacct ttaccacgcg gtccgccccc tttgccagag
    33121 cggtgtacag cacgtcgtca acgtcaggtg cgtcgagagc cgccaccgtc acgctgccgc
    33181 cataacgctc cttcaagatc agcgcctctt cgagggcgtg atcgtccggt tcgctgagga
    33241 tcatccgtag aaactcgagg tccagcccct tcccgtccgg ggcgacctcc agttcttcga
    33301 cgacatccgg gaccatcttg agaagaacaa ggattttcat cttcttctag acctccaaag
    33361 actcggccag cagttccgtc acgtccctga cgatcatccg attgtcgtgg ccgagtacct
    33421 ttagtgcatc ttcgaatcgc gaaacttcgt acggacaaca caccgccaga acatcggcgc
    33481 cggtggcgat ggcttccttg attcgccgtt ccgacagccg gtcgtacccc ttcgccttgt
    33541 agtaggtgtc gagccacatg ccgccgccac ccccgccgca gcacactgcg ttcatccggt
    33601 tgtgcttcat ctccgcgagc ttgatgcccg ggatcgccat caagagttgt cgcggttctt
    33661 cgtagacgtc gttgtagcgg cccatgcagc acgggtcgtg atacgtcacg gtgtagttca
    33721 gctttcgctt cagcatcggc ttcagtcggt cgagatggcg cgctaagtac ggaaccgtgt
    33781 gctccagttc ccaccagaag ccgagtttcc ggtaacggtt catgaatgcg ttgtgcgcgt
    33841 gcggacacga ggtcaccagg cggttgaatt cgtatttctg gaacaccgcc atgttgtagt
    33901 cggtcagtgt ctcgaacagg cccggctccc acgtcaggcg cccgcactcg cctgcgcact
    33961 tctcttcgtt gccgagaatg gcgaagtcac tgccgatcgc gtgaaagagc ttggcggtgg
    34021 cccggctgtt gtcctgccca cgtgggtaga aggacgtata acactcgacg aaccacagca
    34081 cgtccaccgg ttgccggtag tcggaaagaa tccgtatcgg tacgtccgcc gtcttcaccc
    34141 acgcggccct cttgcgcgac gactcgccca ttgggttgcc gtaccgcagc gtgttttcca
    34201 gcgccttttt cagttccgcc ggcaggtccg gcaggtgtac cagcgtctgc tccttcatca
    34261 acggcagcag cacctcggtg agccggatgt cgcgcgggca cttcgccatg cacgcgtagc
    34321 atgccacgca cgccaggaga ctttcgctgg tgaacacctt ctccagcaaa ctcgcccgga
    34381 gcatcgcaat catcttgcgc ggcgggtact ccatcacgtc cccgtaagga cacgtgcccg
    34441 cacacacccc gcactgaatg caggtcagca gccgcttgcc ttcaggtgtg tcgtacaagt
    34501 ttccgagggt aatctggcag gttggttcgg tctcgaccgg agcggtcccc aacatccgtc
    34561 tcctcccgca ggatccactt tcagaccggg tgatctggcc ggccggaacc ctgcttgtgg
    34621 ccccggcggc cgattcccca agcaaagcgt agccggagac cgccccaatt atatcaacag
    34681 gtcttaagac cctaaatact tttttcgtga agtattagta acggagaata ttggcagatt
    34741 tctcgacaca ccgatcaggt cgagactggg atttttcaac actgggcaga tgaggggagc
    34801 ggagcccagc cgcgcgtccc gatcacccag ttgttcgcgg ccttggcact ctttgcggga
    34861 gactctttac acgtactccc agttacgcag ccacttatgg tccggagaga ggttcgccca
    34921 catctccttg caggccttct cgagttccgc tttttccttg agatccagct tgcggtgctc
    34981 ctgcacggcc agcgccatgt tgtctacctg gtgggagttg atcatcccgg gaatcggcgc
    35041 cgtgatcgcc gggttgctga ggatgtacct gatcgcgagg cgcgcccgcc ggtcgtcctc
    35101 cttcgcgtac gggccgttga gggtcccgtc tcccacgaag agcgcgttgt ccgcaaacgg
    35161 cttgatgccg aagacgccta cgttgttcgc cttgatcgcg tcaaaaaccg tgtcgtccgg
    35221 tagctctttg gtgttggcgg tgtacgggaa cagcaccacc tccaactgct tcggagaaga
    35281 ctggttagct tggtatgagg ctttcttgtc agcgccaacc ggcctcatga atccgggccg
    35341 cgagcgttta tgagatgggt tctagtctag tgtttgagtg aactgcgcat ttcttgcacg
    35401 agcaagccag gacaagcgct gctctgcata gacctctatc ggggccatca tcttggcacg
    35461 gcgccaggaa gcggttaggc ttcgccagac caatatttcg ccgacccgtc gtgaaacgct
    35521 accgatccgc ggagcacaat ataaccggga atcgacccga actgagacgg ttattggttc
    35581 ggccagtgga aaaaaggtcc agaacttctt ggaatatggg cggcaaggcc aaccttcccg
    35641 aactgacacc gagggaaaaa caggtctacg atcttgtgat tttgggaaaa cccgataaag
    35701 atatcgcgtc cgatctagga ataagcctcc gcacagcgaa attccatgtg agcaatattt
    35761 accgcaaata tggcgtttca agtcgcattc aactgattct gcgcgaacgg aacatgtctt
    35821 tgaacaccag ccactgaagt ttgcaagtcc gcccatattc tttccctttc tcggctgcgg
    35881 ccccaagggg cgcggtgcac ggatgtcatg ccgactggca ccaaatgcgg ccaagaaacg
    35941 gcaaacctgt ccttcaggac aggagacaaa ttagagattt ttggatatgt ttatctctga
    36001 tgccaaacca aaacgcagct tgcctaaaga agcaattccc tcgaaagttg cttcgctttt
    36061 gcgtcaattg gattcgtaag gagggctgac ttgtcctaca aaaggtgtac atctgctgcc
    36121 gtttggatag ccgggcttct ggccgaggcg gccgctgcag ccgtcattct cgggattctc
    36181 tcgaccgcgc aggcggcagt cccttatcct ccgccgacta cgtggaaagg tgtaaactac
    36241 tcgccgcgga ggcacaccta tttccgaatg ctttacgatt ggtactcgtg ggacagcacc
    36301 gctggaaagt acgttcatca gatggtggac gcggacctga cgcgtctgag ccagaacggg
    36361 tacaacctgt tacatctgta cttgtgggat caggaactcc tccgcagcgt caatgctgcc
    36421 gaacctgctg gtttcttgtc ggccccctcc gacccgagcg gatcgcctaa caaccagtgg
    36481 ggagcgctga acgattttgt cacgaaagcg gagagcaagg gcctcttcgt cgctttgcac
    36541 tttgcaagcg gccggttcat ccatcggatg caaggcaacc caggcggtac cccggaccag
    36601 gtggcaacag agtttgccaa ctggaccgag aagttcattc ggtatctgaa ttccacaaat
    36661 ccccacaaca atgtcctcct ctgggggttg gcctactcca tcgccccggt gcccggtgac
    36721 acaacgggcg attggagcac gacgtggaaa cttgcctaca agaaggtcga tgactatgct
    36781 cgcatgtact ctcctcaccc gagtgtgttg ggactcattg gtacaaacct gtacatggac
    36841 caactgcggc ccgacgggca gccctgggac ggcttcatct atccccgcag tcagggatac
    36901 acgtggaact ggcaagaagc ccagaggatg gcaaagacca tgcgagatct cctcacgtcg
    36961 gcgtacggga cccaaaagga ccctgacatt tatatgatgc agctctatca tccgaactcc
    37021 tacgacttgc ttctgcacct gagctcacta tcctcgggtt atgtagcaaa cggcataccg
    37081 gcacctgtcg ctaaaatatt cgtggtcgaa ttcgccactt cttcctcgct caacagtcct
    37141 cccaacggga acaacatccc gtactacggc gacgctcaga ccccaacgac gaccgtaagc
    37201 ggtcattcac agtggctgaa caacacgttg tgcgcgtacc gttcagctgg gctccagaaa
    37261 tatgcatact ggtcaatgtt cgatccctac accatgtgga ccgggtcacc atggtacgta
    37321 accggccaag atctcgcttg gagtggcttt tggggacttt cgttcgagcc agaagcttct
    37381 ggcgacaagc cctcctggtc ccttctgagc tctttctacc tgaacggctc catcaactgc
    37441 ccctcacccg gtcaaagcaa cgcaccggcg gttgtctcgc tcacctcaag cagcaactac
    37501 tacaccgtcg ctcaaccagt tcggctcacg tggacggcgg ccgatgtggc atcgttttcg
    37561 ctgaaccggg gccacggcgc cagttattcc tgtagcccgg aacagaccgg tcaagtgtca
    37621 agtgacgtgg ctggtagttg tgcgtacacc gatgcttcgg ccttctcgtc gacgggaagc
    37681 cagaccgtga ccctcaccgg gtacaacgtg tggggatctc cgcagaacgc ctcggccagc
    37741 gtcaccatcg ggctgggtcc gatcgtgaac gctgcgacag accagaacta cacctacaca
    37801 atcaacgctt acgacaccat catcgtctgg ggcaatggtt tttctttgac tggtggcaac
    37861 acgattcaat tgacaagagc aggttatccc gacgtgtgga tgtataacgg agatggtcac
    37921 tatttctggg accactccca ttttcagatc aacgcttcac tggatgggag ggcggctaca
    37981 ggtcagtgga cgctgtacgt ccgcaatgga tactccggca cgccctcggc ggcgtatacg
    38041 atcatgatca atccctgagt ttcaggcaag cacgcaaatg ttccggaacc aagaacttgg
    38101 aggttttcaa tgcgcacttt tcttgctttg tttctaggat gtgtctttct tccggtctgc
    38161 ctgtcggcgc agactggtgg gcgcgtctct ggggccatcc tatcggatga aaccggggcg
    38221 ccgatcgtag gggcgaccgt tgtggccgtg cagcagagct caaactttgg gaaagtcggg
    38281 ccggcaattt tccgttcaac gacggaattg gacggcagct atgcgatcgc aaacgtgcct
    38341 tccggagact atctcctttg tctttacgag gctggcgact accttgatcc gtgccagtgg
    38401 ggtcccgcga ccaacgctac gatcgcaacg ggaacgacgt cactggacat cagggtacgc
    38461 aagggcgtcc gcctggcggt tcgggtgggc gacgccgaga acctcgtggc tcagtacaag
    38521 aaaagctcgc cggatgcgtc agctattccg ggagccttca tccggataac tgtaactgac
    38581 acgcaaggta agatgagaac gcttcctctt acctatgcaa gcgagcgact ctacgagtat
    38641 tccaccttgg ttcccccgga caccgatctt actttgaacg tctccagcag cttcttcatg
    38701 ctggcggacc agtcggacaa cccaatcggt gaacagggct attccgcacc gctacggctg
    38761 tcaattccga agccaccgga cccccgcatc cgtctgccat ttgccttctt ggatcgtcgg
    38821 gccaccgtga ttccggtcaa gattcgcgct gcggccagcg gggcgccgta ggtcgtcggc
    38881 tctcgaagtc ttcgtgattc gatgtagact tgaagcaaag gattggagaa tgacttcaga
    38941 tcggacttgg tcagtccttt ttctgacgat tttggcgtcg tgctttaccg tcgaagccga
    39001 gatccgaggt gtcgtggcga cgtccggcgc gagctttgaa ctcgggctgc cggccaaggg
    39061 atcgatcgcc tccatattct gcgccgggat cgaagtcgat ggaatcgtcg cagccgactc
    39121 gctgcccctc ccgagaaccc tcgccggagt gcgcgtctgg gtcggcggag ccccagcccc
    39181 cctgtttgca gtagcggcgt tgcaggggtt tcaacagatc aacattcagg tgccgcatga
    39241 agccgagttc ggcgcggagg ccgcggaagt ggtggtcgag cagaacgggc agagcggaac
    39301 cgcgacagtt cctctgcggc tgtcgacccc cggagacttc tttcgctttg cgggaagcaa
    39361 tctcggcatc ttccagcacg ccagtgacta ttcggtggtg actcctgaaa atcccgcccg
    39421 ccccagcgaa gcgatcatcg cttacctgac cggcatgccg ggtacggaac ccaccgtgcc
    39481 gaccggtgag cctgcgccgt tcgacccgct ggcggttgta ccgcagtacc gttacgccaa
    39541 cgcatatgag cactacgaag tcttcattgg cacgagttcg ctgggagtag agccttcttt
    39601 tgtcggtttc actcccggcc tcgtgggcgt ctaccagttg aacttcgtgg tgccgagcga
    39661 ccggccgagc gccatggcgc agcttgtgtt ggttcgaatc agttgtggag gcttctttgg
    39721 gtcgtgtcca ggaaaccccc tcttcacgcg gacctataag agcactccgg tgacgatccc
    39781 aatcaaatag caggtagcgg ttgaagccat ccgtgatcgt gagcgggtcg tagcacgtgc
    39841 catcgccgca ccagaaatgc cctttgaaat ccgctgacca gacctagtct ggaccgtcaa
    39901 agaaccgcga ccgagaggga gcggcgccac gtcgaacgtt ctcatcgccc tcttcttcgc
    39961 ggcattggcg ctctttgtgg gaaattcttt acacgtactc ccagttgcgc agccaggtgt
    40021 ggtcgggcga gaggttcgcc cacatctcct tgcaagcttt ctcgagttcc gctttttcct
    40081 tgagatccag cttccggcgc tcctgtaccg ccagcgccat gttgtccacc tggtgggtgt
    40141 tgatcatccc cggaatcggc gccgtgagtg ccgggttgct gaggatgtag cggatcgcca
    40201 ggcgcgcccg gcgatcgtct tccttcgcgt acggtccgtt gagggtcccg tctcccacga
    40261 acagcgcgtt gtcggcaaac ggcttgatac cgaagacgcc cacgttgttc gccttgatcg
    40321 cgtcgaagac cgtgtcgtcc ggcagctctt tcgtattcgc ggtgtacggg aacagcacca
    40381 cctccaactg tttcggatac tgctcgatga gcgacttcag ccacacccgg ttgtgcgtcg
    40441 aaatcccggt gaaccggact tttccctgtt tcttcgcctt ttccagcgcc tccatggcgc
    40501 cctgctcgac tcccagcagc tcgtcgaggc tctggatcat atccatcggc aggctgatcc
    40561 gccagatgtc cacgtagtcg agcttggcct ccttcatgcc ctcgtccagc ccggccagca
    40621 gcttcgttcc cgcgcgccac tccatgtacc gcgactcccg cacgtgccac gagtagccca
    40681 ggtacatctt ctgccgccgc cctcggagca cacggctgta cgccatgatc tcaggccccg
    40741 cgcaggcgtc gatatagttg atgcccactt ccatgcagcg gctcaccacg gcgtcccggc
    40801 tcttcaggaa gtccgagtcc tggatgttct ccatgtcgtt ctcggagtaa ccgacgctgg
    40861 tcttcagctt cccgccgagc atcgtgtgca cgcgcttcca atgcccgccc aggcataccg
    40921 ccgacaccat catcccagtc ttgccgagct tccggtattc catgttcggg ttgtaattca
    40981 ggatgctccg cgtgttcacc ggcgtggcgt tcgccgccgc caccccggcc accgccgctc
    41041 cgccggccaa gaagccccgc cgcgtcatgg gagtgtcgct catagttttg cctccgttaa
    41101 tcttcaacta tatcccaggt ttcccgcccc atgtacaatg attttctttc atcgttcttc
    41161 agaggatctc tgaacttgcg gcgattcatc ctctccattc tctttgcggc ctgcgtccta
    41221 cgtgcggcgc tccccacgcc cgagagctac ttcggtcatc gcatgggcgc cgaccggcgc
    41281 gtgctcgact ggagccgggt cgtctcctat tttcaggagc tcgatcgctc cagcgaccgc
    41341 atcgtcgtga aggaactggg caagagcacc gaggggaggc ccttcctcgc cgccttcatc
    41401 gcctccccag cgacgctccg cgatctcgat cgcttccggt ccatccagaa gcgcctcgcc
    41461 gacccacgta agaccagccg cgcggacgcc gagccgctga tcggccgggg cagggcagtg
    41521 gtcatgatca cctgctccat tcacgcgacc gagatcgcct ccagccacac cgccgtcgag
    41581 ttcgcttggc gcctgctcac ggacgacagc ccgaaagtac gtgcgatact cgacaacgtc
    41641 atcttgatcc tcgtgccgtc gctgaacccg gacgggatgg atctcgtcgc ggactggtac
    41701 cggaaaaccc tcggcactcc gttcgaaggc acgtctcccc cgcacctcta ccagaagtac
    41761 accggccacg acaacaaccg tgactggtac atcttctctc agcaggagac ccggctcgtc
    41821 gtctcgcagt tgcacaacgc ctggcatccg cacatcgtct acgacgttca tcaacaggga
    41881 ccgaacgcgt ctcgcatgtt cgtgccgccc tggctcgatc ccgtcgaccc gaacatcgat
    41941 ccgctcatcc tccaggagtg caacctcttc ggaatgggaa tggccgccgg cctcaccggc
    42001 gccggcaaga ccggtgtagt cgtgaacgcc ctgtacgatt tctggacgcc cgcccgccac
    42061 taccaggcct accacggtgg tctccggatc ctgagcgaat cggcttccgc ccggattgcc
    42121 acgcccgtga ccgttcgacc cgaccagatc gatcgcaccg ccctcggcta caacccgcgc
    42181 gagtcgagct ggaactatct cgagccttgg cttggtggcg agtggcgctt gcgcgacatc
    42241 gtcgactacc agtccatcgc ctgggagtcc cttctcaccc aggccgcatc ctgccgcgag
    42301 accctgctgc gcaacttcta ttgcatcaac cagcgtgcgg ccgcgcgcac ctcgccttac
    42361 gccttcgtcg tatccgcgtc gcagtccgac cccggtgccg cacgaaagtt gctcgaaacg
    42421 ctggtgttcg gcttggtgga aatcgggcgc gcccgcgggg cgttcgaagc cgtcggcaaa
    42481 cgctaccccg caagcagcta cgtcatccgg atgcagcagc ccttcagttc gtttgcgaag
    42541 acgttactcg agcgccagcg ataccccgac ctccgtgtct atcctggagg accgcccagg
    42601 cgcccgtatg acgtcaccgc gcacactctc cccctgctaa tgggcgtcgc tgtcgactcc
    42661 gtcgagggtc cgttcgatgc gccgctcgat ctcgtctccc ggttcgaacc acctccgaag
    42721 aaccgcccca ttcttccggc gtccgacacc gattcctggc gggaagtgaa tcgtgtctgg
    42781 cgcgccggcg gcacggtctg gcgcgacccg gctacgggcg acttctacac cgccctgagg
    42841 cccgggctca tacagctcca tcgcccgcgc atcggcttgt atcgcagcca cgttccaagc
    42901 atggacgaag gctggacccg ctggctgctg gaggaattcg gattcgccta cgaaagcgtc
    42961 ggcaacaccg atatcgaggc aagctccctg cgccctcgct tcgaggtcat cgtgttcccc
    43021 gaccagtctt cctccgaaat cgaggagggg ttccggaaag gggaaatgcc cgacgaatac
    43081 tgcggcggat tgggcgactc tggcgcggag gcgctccgag cgttcgcccg agacggcggc
    43141 acgctcatct ttctgaatca ctccaccgcc tgggcacgcg cgcatctcgg tctggatgtc
    43201 gtcgacctgc tcgaaggcct cccgaacacc gaatactact cacccggctc gttgctcaat
    43261 gtcaccctgg aatcgaagag cccgctcact ctcggcctgc ccgaacgcat cgcgatctgg
    43321 tctcagggca gtccggcttg gcgggcgccc gatgccaccg tcgtcgcccg ctattcgcaa
    43381 tcggacatgc tcgcctcggg ctggcttctt ggcgagaagc gtctgtccgg ccaagctgcg
    43441 ctcgtcgacc tccccatcgg ttcaggccgc gttatcctct tcggcatgcg cccgcagtac
    43501 cgcgcccaaa gctatcaggc ctttaagctc ctcttcaacg ccttattgct gggcgcgtga
    43561 cccgtttccg ctatcagcca ccttcaactc agcccccgag ctataattcc cccttgatgt
    43621 cgccggatct cctccatctg cacacctact tcctgtttcc gttctctatc gacaaacagg
    43681 cggtgctgga ggaccaccgc gacctctggg cgaagtaccc tcgttggatc gacggtctgg
    43741 acgagtggat cgccgtgcat cgcacgcggg ccagttccac cgtggcccag cgtcttggcc
    43801 cctggcggcg cgcggcctat agccgcttcg acctcgactc ctccgcctac caggacatgg
    43861 tcttctttca ccctatcgtc cgccgagtgt tcttcgacac caccgacgca gcgggcggag
    43921 aaaaggaag
//
LOCUS       DATIFQ010000088        14843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2517794, whole genome shotgun sequence.
ACCESSION   DATIFQ010000088 DATIFQ010000000
VERSION     DATIFQ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14843
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2517794"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1535
                     /locus_tag="VLE22_03910"
     CDS             216..1535
                     /locus_tag="VLE22_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13577.1"
                     /translation="MAAPATASLLPQDKAKVRLVFTHPDPKLEGWPYQGYDYESRKAK
                     YAAKLLEGCPGIEFLPDTAATEADAKRILSRDAEVDGYLIYMFGIPAQANPVFAFSSR
                     PALMVDDLYGGTGSFLGAYPKARAKGMPVAGVSSSRFEDIVEAANAFAAIKKLRSSAM
                     LQVAENDQAKAIALYGQTLGLTVRQVRAEELNQAYEGVDRAEVRRWAKGWIERADRVI
                     EPSSAEIEKSAAMYLGMRDLLAKYKAQGIAVDCLRLFYGHKITAYPCLGFMQLNDDGL
                     VGACEADLQSASTMLMLAYLTGRPGFISDPVIDTSNNQIIYAHCVAPTKVFGPDGPST
                     RYEIRSHSEDRKGAAVRSLMPLGEMTTTLKIVPDQKLVVIHRAKTVANLEEDRACRTK
                     LAAEVPDARKLLNDWDFGWHRVTVYGDAKVQVETITGLMGLKFVEEG"
     gene            complement(1544..2566)
                     /locus_tag="VLE22_03915"
     CDS             complement(1544..2566)
                     /locus_tag="VLE22_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HSB13578.1"
                     /translation="MKRRIFLMGLAGLAGCTSGRRPRLNVFNWSNYVAPDTIADFESE
                     FGVRVRYAVYESNEEMLAKVISGNSGWDVVFPSNYFIRPMREQRLLTPLRHDWLPELS
                     ALRGTFRSPSWDPVLQWCVPYMWGGTGIVYNRKLTPPPASWSFLWDARLAGRITMLDD
                     PSEVLGAALKKLGVSVNSTDPAQLRRAQQEALAQKPLLRAYLNAEVRDQLVAGDVLSA
                     QLWTNTAQQALDGSPDLGFVFPSEGFPLYADNAAILRESRHPELAHRFINYLLRPRVS
                     ASIAGAARAATANGAAQALLPDAVRQNPTLYPPPEVLARGEWWETPPAAIQRLRDRLW
                     TEIKSA"
     gene            complement(2563..3321)
                     /locus_tag="VLE22_03920"
     CDS             complement(2563..3321)
                     /locus_tag="VLE22_03920"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB13579.1"
                     /translation="MKRLLALYAVIAYAFLHLPLLILTVFSFNASRFTVWEGFSLDWY
                     AAAVRDPQLVEAAFNSLVIAVVATVISTVIGTLCAYGLWKRGSRWFSGTLYLSLVTPE
                     IVMGISLLAFYQWLFRFFGARLGMHTVIGAHVAFSIAFVVVVVIARLRTFDATLEEAA
                     MDLGATEWQAFRSVTLPALAPAVGASALLAFTLSFDDYVITSLVAGVDSETLPMVIYA
                     MARRGANPVVNAVSAFIVLGLTVLILAAEKLRET"
     gene            complement(3318..4148)
                     /locus_tag="VLE22_03925"
     CDS             complement(3318..4148)
                     /locus_tag="VLE22_03925"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB13580.1"
                     /translation="MTRLRLYFLTPAALSLTLLFLAPLAIVLVYSLMNRGVWGGVELP
                     YTVENYRRLLDPIYLGILLRSFLMAGAATLACLLLGFPLALFIARAGKRQSLYLHLVI
                     VPFWTSFLVRTYAWMFLLRDTGLVNTALLALGFIREPLPLLYNSFAVLVGLVYGYLPF
                     MVLPLYATLAKIDRSLLEAAADLGARPWQSLRRVIVPLAAPGIRAGAVLVFIPCLGAY
                     LTPDLLGGSKTIMIGNVIQNQFTSSRDWPFGSAVSLAVMAVVMALLLGWLRREKERLL
                     "
     gene            complement(4145..5143)
                     /locus_tag="VLE22_03930"
     CDS             complement(4145..5143)
                     /locus_tag="VLE22_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB13581.1"
                     /translation="MRSALLSLQMVPVLELRGLSKRYPGHTAVDGISLSIPRGAFYSL
                     LGPSGCGKTTTLRLIAGLEAPDSGEIYLNGESISHLRPYERNVNTVFQNYALFPHLTV
                     EGNIGFGLRHKRSDDIGRRVRETLQLVQLEGKENRYPSELSGGERQRAALARSLVLEP
                     AVLLLDEPLAALDQKLRKQMRAELKSLQRRVDITFLFVTHDQEEALSLSDCIAVMNQG
                     RIEQIGAPQEVYLRPRTRFVAGFLGAVNWINGAGVRPETTHISRVIPEGDVRVCPATV
                     ESSVFLGNCFHVEARLATGIPVIAEVPRHDDTYESGESVHVWWRTSDELTFEHESE"
     gene            5235..7841
                     /locus_tag="VLE22_03935"
     CDS             5235..7841
                     /locus_tag="VLE22_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB13582.1"
                     /translation="MNRRCVSSLALWAVLCGGIVIGAENEGVVRSGGLPIPGATVTAT
                     QREKSVTTVTDEQGRYSFSDLTAGAWSIEVQMFGFMPKTQTVTVPSPRPVEWDLTLAA
                     MAALGPQGRPADNRRLPMNRTAAAMMTSIDTADLAVSSQEANESFLVSGSQSSGLGMQ
                     PQWPFGFGGPPGGPSVFGPGGMGGPPAGMEPGFGGGRSGGGFGGGMGPGMPGGGGMGR
                     PGRPEGGPPGGDMGRMRGMRGGPPGGRRPDWMARRGVAVFGNRRPPNQSAIRGHAFFS
                     LNNSALDARPYSISGQQVAKPSYNQVRFGIGAGGALRIPKLISGSRTFFFVNYSGTRG
                     RSAFDRISTVPSPAERLGDFSQSVARGSVVVFDPLTNQPFPDNQIPSARFDRAASGLL
                     SYIPLPNLSSRVQNYQFVASVPQSSDNLSLRLNHSLTGKDQLSGSFNLQNRHSETAQL
                     FGFQDETNGRGASVNIGWTHNLTTTMINDLRFQFSRNRSEALPYFAYRVDVAGDLGIQ
                     GTSRDPINNGPPNLAFTNFGALTDGSPLLRRNQSSGFTEGVTLVRGDHTFRFGFEYRR
                     NQLNTRTDENGRGSYSFSGLITSAFDTSGNPLPDTGFDMADFLLGRPQSSTIRWGNSD
                     MYFRAGNYNFHVVDDWHARPNLTLNIGMRYEYSEPLYEKYNRMANLDIAPGYTGVALV
                     TPTAAGTYSGLFPRGLIEPDRNNFSPRAGIAWKLWPKQSLIVRAGYGIYYNGSVYNTI
                     ASLLGQQPPFANTNAFTTSIENPLTIAEGFLGIPDTRITNTYAVDRGYLVGYAQTWNL
                     SVEKSLPFALTMEIGYSGTKGTRLDVQRVPNQAATGSPLNSENRRPIPYAVGSHLTVR
                     TGIRFTTPARCG"
     gene            7862..8527
                     /locus_tag="VLE22_03940"
     CDS             7862..8527
                     /locus_tag="VLE22_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13583.1"
                     /translation="MSFFTVYRWSKSIDDVSTYGSGGTVVAQDPFDLSAERGLSSFDQ
                     RHSLDATFILTSPFGGGPAAVHMEGLSRRLLEDWTLGGGMALHSGTPLTAQVRGNRSD
                     TGGTGVVGSGRADASGMPVDAGSGFFNREAFTVPPSGRYGNAGRNTIPGPDLFSLNLE
                     FGRSFPLWSDRRSAEFRVEANNPLNSVSFSGFGTTVNASDYGRPTAAASMRKVTAQLR
                     FRF"
     gene            8608..10608
                     /locus_tag="VLE22_03945"
     CDS             8608..10608
                     /locus_tag="VLE22_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB13584.1"
                     /translation="MTTRLVVVNVEVKDRNGRPAENLTRDDFELTEDGKAQKIAVFDY
                     QRLETKPGTTSPAVGARPGKSRNEITPSTPGEFRYRDHRLLVLYFDLSSMPAEDQIRA
                     RTAAEKFIREEMTPVDLVAIMSFSSELKVLQDFTDDRDRLMQTLAELRIGETSDLAGQ
                     AATGETEESQDTGAAFSADETEFNIFNTDRKLSALESATRMLASLPEKKGLVYFSSGV
                     GRTGVENQSQLRATVNAAVRANVSFYSVDARGLVAEAPLGDARSGGNRGSSMYSGGAQ
                     RGRRDTFLNQQETLFTLAADTGGKALLDSNDLSIGIVQAQSDISSYYTLGYYSTNTAE
                     DGKYRRILVRVKAVEKAKLDYRSGYFAAKRFEDFSASERERQLEEALLLEDPITDLNL
                     ALEVDYFRRDRDRYIVPLAVKIPGSQIRLAQKSGAETTRLDFIGQVRNIRGFLMGTVR
                     DHIQVTLRGENSAQLSRRNLEYDSAFTLPPGEYVLKFLVRENETGKMGTFETRFEIPN
                     LDSEKSYLKLSSVVWSNQREPMSEAVGGVKRDKRLVKNDPLVRDREKLIPSITKVFRK
                     DQTLRVFFEVYNPGVVNGEKTANLTANVSLYRAQAKVFESEAIRVSEGQGNRYFLPVE
                     FEIPLANLETGRYVCQLNVIDEAGRKFAFRRTPLMLLPAAPL"
     gene            10663..11469
                     /locus_tag="VLE22_03950"
     CDS             10663..11469
                     /locus_tag="VLE22_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13585.1"
                     /translation="MQYLCGSARLTRRAVLTFTAAASASLWNRLFAANSDFWNRKKPE
                     DWSSDEIERLVTKSPWAKEVTAEFNQPERGGRGGGMGVPGGGMGGPTTRRGGMGMPPI
                     GGGGMGIPPLGGGGRDGRPSGGMERRKVTVRWESAKPIREALKTPLPDSLADRYVISV
                     NGLPMMPGSGRRREDEGSEDTSRAPRNMLERLKETTFLQPKGKESVQPGVVERQPSAG
                     GASLLFGFSRETLVLHPEDKEVTFTMAMGPLEVKTKFKLTDMTYREELAL"
     gene            complement(11547..12980)
                     /locus_tag="VLE22_03955"
     CDS             complement(11547..12980)
                     /locus_tag="VLE22_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB13586.1"
                     /translation="MRTDFEQLYSERLNRYVTAMRNGMPDRVPLRPFAAEITARHAGF
                     TCQEVTHDYNKAFEAVIRCCKDYNWDAVVPNMVYVWTGLTQAIGLRYYGVPGIDVGPD
                     VGFQYREPSEREAFMKREEYDDLIEDPTRFLYETWLPRVTGEVSAKGTPVTYRNNLSF
                     VKGGMSMLSYFAAFGPQVERMRRETGTVSSICGMLKAPLDILADKLRGYIGLAFDLKE
                     IPEKVVAACHALMPHLGYLALSSADPGKSVPIPIWMHRGCVPFISREHFETIYWPTLK
                     PIVEALWAQGNQVLFYAEGKWDAHLERFAELPAGSIIFHIDRGDPDRVHRVLGRKFCL
                     SGGVPNALLTLGKPEQVKAQCRKLIETIGAEGGYIMDASAIMQNDATLENLKAMTDAT
                     LEYGCYRSPSSPSTKARVEPRWEAPTPGIPGWVTAPPVRPGVCLPWEEKLKELPPMCG
                     DPSLVRSVWESNEGFAYLYIWHLLLSF"
     gene            complement(13054..14118)
                     /locus_tag="VLE22_03960"
     CDS             complement(13054..14118)
                     /locus_tag="VLE22_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HSB13587.1"
                     /translation="MRSFDEAPFVVIWEVTQACDLACLHCRASAIACRNPLELATNEG
                     FRLLEEIRTFGDPLMVFTGGDPLKRPDLFDLLRHSVSLGLRTTVTPSATPLLTAEAIQ
                     GFRDCGVARMAVSLDGPDAALHDGFRRVDGSFERTIFALEHARRAGLPTQINTTVTRH
                     NFGQLEEIATLVAHWDARLWSVFFLVVTGRAMRSDDLTPEEYEDVFRFLYELSKTAPF
                     DIKTTEAQHYRRYVAQRRKAEGGAANRSTAGDVIQRQAGINDGKGFVFVSHTGEIFPS
                     GFLPLAAGNVRRDSIAEVYRNSALFRSLRNSDNLLGKCGDCEYRNLCGGSRSRAYALT
                     GNYLESDPNCVYQPRLATVA"
     gene            14220..14795
                     /locus_tag="VLE22_03965"
     CDS             14220..14795
                     /locus_tag="VLE22_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSB13588.1"
                     /translation="MDLAENKTAATVPGEGADWSSAPLHSLMDHIVGIHHEYLKLELP
                     RLADKAGRLVKSRIEKYGDSSLVPLLDTFLELKSELESHMWKEEVVLFPLIRNMEAAA
                     TAGVKAPPAHCGSVNNPIRVMEHEHASAERALEEMRRITSNYAVPEGACETHRMLFEA
                     LRVLEEDLCRHIRLENDILHPRASELEMLVS"
BASE COUNT         2976 a         4343 c         4849 g         2675 t
ORIGIN      
        1 ccagcgctgc gagccacccg ggttgacccg atggtcgcgc tccgccagga gtgactggcc
       61 gagcctatgg aacctgtccg actgatgtat actcggcggg caaagccatg aaaccgctta
      121 atgttcttac ctcttccagc cgcagaagct tcctttcgaa gtgctgcgga gcggccgcat
      181 gcgccgcgtg tcccgccgtg ttgcggcgtc cgttgatggc cgcgcccgct acggcgtcgc
      241 tcctgccaca ggacaaggcg aaagtccgcc tggtcttcac gcaccccgac cccaagcttg
      301 aaggctggcc ctatcagggc tacgactatg agagccgcaa ggcgaagtac gcagcaaagc
      361 tgctcgaggg ttgtcccggg attgagtttc ttcccgacac cgcagccacc gaggccgacg
      421 caaaacgcat tctttcgcgt gatgcggagg tggacggcta cctgatctac atgttcggta
      481 tccctgccca ggccaatccg gtgttcgcat tctcgagtcg gccggcgctg atggtagatg
      541 acttgtacgg aggtaccggc tcgtttctcg gcgcgtatcc gaaggcgcgc gccaagggaa
      601 tgcccgtggc tggcgtttcg tccagtcggt tcgaggatat tgtcgaagct gcgaatgcgt
      661 tcgcagccat caagaaactt cgcagttccg cgatgcttca ggtggccgaa aacgaccagg
      721 caaaagcaat cgcgttgtac ggccagacgc tggggcttac ggtccggcag gtccgcgcgg
      781 aagagctgaa ccaagcgtac gaaggtgtcg atcgcgccga ggtacggcgt tgggccaagg
      841 gctggatcga gcgtgccgac cgggtgatcg agccgtcgtc ggcggagatc gaaaagtcgg
      901 cggcgatgta cctgggcatg cgcgatctgc tggcaaagta caaggcgcag ggaatcgcgg
      961 tggactgcct gcggctgttt tacgggcaca agatcacggc atacccatgc cttggtttca
     1021 tgcaactcaa cgacgacggg ctggtggggg cctgcgaagc cgacctgcaa tcggcgtcaa
     1081 caatgctgat gctggcgtac ctgacgggac ggcccggttt catctcggat ccggtgatcg
     1141 acacgtcgaa caaccagatc atctatgcgc actgcgtggc gccgacgaag gtgttcgggc
     1201 cggacgggcc gtcgacgcgg tacgagattc ggagccactc cgaagaccgg aagggcgcgg
     1261 cggttcgctc gctgatgccg ctcggcgaga tgacgacgac gttgaagatc gtgccggacc
     1321 agaagctggt cgtgattcac cgggccaaaa cggtggcgaa cctcgaggag gaccgcgcct
     1381 gccggacgaa gctggcggcc gaggttccgg acgcgcggaa gctcctcaac gactgggatt
     1441 tcggctggca ccgcgtgacc gtctacggtg atgcaaaagt tcaggtcgaa accattaccg
     1501 gattgatggg attgaagttc gtggaggagg gatagctaga gactcacgcc gatttgattt
     1561 cggtccagag gcggtcgcgg agacgctgga tggcggcggg cggcgtctcc caccactcgc
     1621 cgcgggcaag cacctcgggc ggcgggtaca gggtcgggtt ctgacgcacg gcgtccggga
     1681 gcagggcctg cgcggcgccg ttggcggtgg cggcgcgcgc cgcaccggcg atcgacgccg
     1741 agacgcgtgg ccgcagcagg tagttgatga agcggtgggc cagctctggg tgtcggctct
     1801 cacggaggat ggccgcgttg tcggcgtaaa gcgggaagcc ctcggacggg aagacgaagc
     1861 cgaggtccgg agaaccgtcc agggcctgct gcgcggtgtt ggtccagagt tgcgcgctaa
     1921 gaacgtcgcc ggcgacgagc tgatcacgca cttcggcgtt aaggtacgcg cgcaggagcg
     1981 gcttttgagc cagcgcttcc tgttgtgcgc ggcggagctg ggcagggtcg gtcgagttga
     2041 ctgaaacccc gagtttcttc agcgccgcgc ccagcacctc cgaggggtcg tcgagcatgg
     2101 tgatccggcc ggccagccgt gcgtcccaga gaaaactcca ggacgcgggc ggaggcgtca
     2161 gcttccggtt gtacacaatg ccggtgccgc cccacatgta cggaacgcac cactggagaa
     2221 ccggatccca cgaaggagac cggaacgttc cgcgaagcgc ggagagttct ggcagccagt
     2281 cgtggcggag cggcgtgagt aatcgctgct cccgcatcgg tcggatgaaa tagttgctcg
     2341 gaaagacgac gtcccagccc gagttgccgg agatgacctt cgccagcatc tcctcgttgc
     2401 tttcgtaaac ggcatagcgg acccgcacgc caaactcact ctcgaaatca gcgatcgtgt
     2461 cgggtgcgac gtagttggac cagttgaaga cgttaaggcg cggtcggcgg ccggaggtgc
     2521 acccggccaa gccggctagg cccatcagaa agatgcggcg cttcacgtct ccctcagctt
     2581 ttccgcggcg agaatcagca ccgtgagccc gagcacgatg aaggccgaga ccgcattcac
     2641 taccggattg gcgccgcgcc gggccatcgc atagatcacc atgggaagcg tttcggagtc
     2701 cacaccggcg accagactgg tgatgacgta atcgtcgaag gaaagggtaa aggcgagcag
     2761 cgctgaggcg ccaactgcgg gtgcaagggc cggcagagtc accgagcgaa aggcctgcca
     2821 ttcggtcgcg ccgagatcca ttgccgcttc ctccagggtt gcgtcgaacg tgcggagccg
     2881 cgcaatcacg accacgacga caaaggcgat cgagaacgcg acgtgcgcgc cgatgaccgt
     2941 gtgcatccca agccgggcgc cgaagaaccg aaacagccac tggtagaagg ccagcagaga
     3001 gattcccatc acgatctcgg gcgtcacgag cgagaggtac agcgtcccgg agaaccatcg
     3061 cgatccccgt ttccacaatc catatgcgca gagcgtaccg attacggtgg agatcacggt
     3121 cgccacaacc gcgatcacga ggctgttgaa cgccgcttcg acaagctggg ggtcacgaac
     3181 cgccgcggcg taccagtcga gagagaagcc ttcccaaacg gtgaagcggg acgcgttgaa
     3241 actaaagacc gtgaggataa gaagcggcag gtgcagaaag gcatacgcaa tgacagcgta
     3301 aagcgcgagc aggcgtttca cagcaaacgc tccttctcgc gccgcagcca cccgaggagc
     3361 agcgccatca ccaccgccat cactgccagc gacaccgcgg agccaaacgg ccagtcgcgc
     3421 gaggaggtga actggttctg gatcacgttg ccgatcatga tcgtcttact gccgccgagc
     3481 aggtcaggag tcagataggc gccgaggcag ggaatgaaga cgagcaccgc tcctgcccga
     3541 atgcctggcg ctgccagtgg cacgatgacg cgccggaggc tctgccacgg ccttgcgcct
     3601 aggtcggccg ccgcttcgag caggctgcgg tcgattttcg cgagggtggc atagagaggc
     3661 agcaccatga aaggaaggta gccgtagacc agaccgacca agaccgcgaa agagttgtag
     3721 agcaggggca ggggttcacg gatgaaaccg agcgcgagca gcgcggtgtt cacaagtcct
     3781 gtgtcacgga gaagaaacat ccaggcgtac gtccggacaa ggaaactggt ccagaacggg
     3841 acaataacca agtggaggta gaggctctgc cgctttcccg cgcgggcgat gaacagcgcg
     3901 aggggaaatc cgagaaggag acacgcgagc gtcgctgcgc cggccatcag aaaggaacgc
     3961 agcaggatgc cgaggtagat cgggtcgagc aggcggcggt agttctcgac ggtgtaagga
     4021 agttccaccc ctccccaaac gccacggttc atcaggctgt agacgagcac gatggcgaga
     4081 ggcgcaagga acaacagcgt gagggaaagc gccgcagggg tgaggaagta cagcctgagc
     4141 cgcgtcattc cgattcgtgc tcgaaggtga gctcgtccga cgtgcgccac cagacgtgga
     4201 cgctttcgcc gctctcgtaa gtgtcgtcgt gacgcggaac ctcggctatg acgggtatgc
     4261 cggtcgcgag ccgggcttcg acgtggaagc agttcccgag gaataccgag ctttcgacgg
     4321 tcgcgggaca gactcgcacg tcaccttccg ggatgacccg agagatgtgg gttgtctcgg
     4381 gacgcacgcc ggcgccgtta atccagttga cggcgccgag gaagccggcg acgaaacgcg
     4441 tccggggccg caggtaaacc tcctgcggcg cgccgatctg ctcaatccgt ccctgattca
     4501 tgacggcgat gcaatcggaa agagagagcg cttcctcctg atcgtgagtg acaaagagga
     4561 aggtgatgtc gacgcggcgc tggaggctct tgagctctgc gcgcatctgc ttgcgcagtt
     4621 tttgatcgag cgcagccagg ggctcgtcca ggagcaacac cgccggctcg aggaccaacg
     4681 agcgggcgag cgctgcccgt tgccgctcgc caccggagag ttcggagggg tagcggttct
     4741 ctttgccttc gagttggact agttgcaggg tctctcgaac gcgccttccg atgtcgtccg
     4801 aacgcttgtg ccgcagaccg aacccgatgt tgccctcgac cgtgaggtgc gggaacagag
     4861 cgtagttctg gaagaccgtg ttgacgttgc gctcgtacgg acgcaggtgg ctgatggact
     4921 caccgttcaa gtagatctcg cccgagtcgg gcgcttcgag gccggcgatc aggcgcagcg
     4981 tcgtggtttt tccgcagccg gaggggccga gcagcgagta aaacgcaccc cttggaatcg
     5041 acagcgagat cccatccacc gccgtgtgtc cgggataacg ttttgacagg cctctcagtt
     5101 ccagaacagg aaccatttgg agagatagta gcgcagaacg catactaatc attcattaga
     5161 aacccaagaa atcccgccgc caagtgtaat cccaggacgc cgtagcgtcg tcttgaatca
     5221 ttagggccag gtttatgaac cgaaggtgcg tctcttcttt ggcgctatgg gccgtgctgt
     5281 gtggcgggat cgtcattggg gccgagaacg aaggcgtcgt ccgttcgggc gggttgccca
     5341 tcccgggagc gaccgtgacc gcgacgcaga gggaaaagtc cgtcacgacg gttacggatg
     5401 aacaaggtag gtactctttt tcggacctca cggcgggcgc ctggagcata gaggtccaaa
     5461 tgttcggctt catgccaaaa acccaaactg ttacggttcc ctccccacga ccggtcgagt
     5521 gggatctcac tctggccgcg atggccgctc tggggccgca aggacggccg gccgacaacc
     5581 gccggctgcc tatgaatcgc accgcggcgg cgatgatgac cagcattgat acggccgatc
     5641 ttgcggtttc ttctcaggaa gcgaatgagt ctttcctggt cagcgggagc caaagcagtg
     5701 ggttggggat gcaaccgcag tggccattcg gattcggggg gccgcccggc ggaccgagtg
     5761 tatttgggcc gggaggaatg ggcggtccgc ccgctggtat ggaaccgggg ttcggcgggg
     5821 gacgatcggg cggcgggttt ggcgggggca tgggtcccgg aatgccgggg ggtggaggaa
     5881 tgggcagacc aggcaggccg gaaggcggtc cgccaggcgg agacatgggt cgtatgcgtg
     5941 gtatgcgcgg cggtcctccc ggtgggaggc gtccagactg gatggcgcgg cgcggtgtgg
     6001 cggtctttgg caatcgtcgg ccacccaatc agtctgccat tcgcggacat gccttctttt
     6061 cgctcaacaa ctcggcgctg gatgcgcgtc cgtactccat ttcaggccag caggtggcta
     6121 agccgtctta caaccaggtg cgtttcggca tcggtgcggg cggagcactc cgtatcccaa
     6181 agctgatcag cggcagcagg acgttcttct tcgtgaatta ctccggcacc cgaggccgga
     6241 gtgcgttcga ccggatctcg acggtaccct cgcctgctga gcgtctcggt gatttctcgc
     6301 agtctgtggc ccgtggctca gtagtggtct tcgacccact caccaaccag ccctttcctg
     6361 acaaccagat tccctcagcg aggtttgacc gggcggcgag tgggctgttg tcttacattc
     6421 cactgcccaa tctgtccagt cgagttcaga actatcagtt tgttgcgtct gtcccgcaga
     6481 gttccgacaa cctcagcctg cggctgaacc atagccttac tgggaaagac cagctctccg
     6541 gttcgttcaa cctgcagaac cggcattcgg agacagcgca gttgttcggc ttccaagacg
     6601 aaaccaacgg gcgtggggca agtgtaaata tcggatggac gcataacctc acgaccacaa
     6661 tgatcaacga tctcagattt caattcagca ggaatcggag tgaggcgctt ccgtacttcg
     6721 cgtaccgtgt cgatgtggct ggcgaccttg gcatccaggg aacttcgcgg gaccccatca
     6781 acaacggccc tccgaaccta gcctttacga actttggcgc tctgacagat ggcagcccgc
     6841 tgctgcgccg gaaccaatcc tccggcttca cggaaggcgt tacgttggtt cggggagatc
     6901 acacctttcg tttcggattc gaataccgcc gcaaccagtt aaacactcgt actgacgaaa
     6961 acgggcgtgg ctcgtacagt ttcagcggct tgattaccag tgccttcgac acctccggga
     7021 atccgctgcc cgacaccggg ttcgacatgg ccgacttcct tctcggtcgc ccgcagtcga
     7081 gcacgatccg ctggggcaac tcggacatgt acttccgcgc tgggaactat aacttccacg
     7141 tggttgacga ctggcacgct cgccccaacc tcacgctcaa tatcggcatg cgatacgagt
     7201 attcggaacc tttgtatgag aaatacaacc ggatggcaaa tctcgacatc gcgccgggct
     7261 acacgggcgt cgccttggtc actccgacgg ctgcgggcac ttactccggc ctgtttccac
     7321 gagggctgat cgagccggac aggaacaatt tttcgccgcg cgccggcatt gcatggaaac
     7381 tgtggccgaa acaatcgctg atcgttcgcg cgggatacgg catttattac aacggctcgg
     7441 tctacaatac gatcgcgtcg ttgctgggcc agcaaccacc gttcgccaac acgaatgcgt
     7501 tcacgacgag catcgagaac ccgctgacga tcgccgaggg tttcctggga atacccgaca
     7561 cgaggatcac gaacacttat gcggttgacc gcggctatct ggtcggttac gcgcagacct
     7621 ggaatctctc cgtcgagaag agcctgccat ttgcgctgac aatggagatc ggctattccg
     7681 gaaccaaggg gacgcggctg gacgtgcagc gggtgccgaa ccaagcggct acgggctcac
     7741 cgctcaattc cgagaatcgg cggcccatcc cgtacgcggt cggttcacac ttgacagtgc
     7801 ggacgggaat tcgatttacc acgccggcca ggtgcggctg atgtggcggt tcaggagagg
     7861 ggtgtcgttc ttcaccgtct acaggtggtc aaagtcgatt gacgatgtat cgacgtacgg
     7921 tagtggcggt accgtggtgg cgcaggatcc cttcgacctc agcgccgagc ggggactgtc
     7981 gagcttcgac cagcggcact cgctggatgc cacgttcatt ctgacttccc cgttcggcgg
     8041 ggggccggcg gcggttcaca tggaagggtt gagccgccga ctgctcgagg actggacgct
     8101 cggcggcgga atggctctcc actcggggac gcctttgacc gctcaagtgc gcggcaaccg
     8161 ctcggacacg ggcggcaccg gtgtagtggg aagcggccgg gcggacgcct caggtatgcc
     8221 ggtcgacgct ggcagtggct tctttaacag ggaggctttc actgtcccgc cgtccggccg
     8281 ttacggcaac gccgggagga acactattcc tggccctgat ctcttctcgc tcaacttgga
     8341 gttcggacgg tctttcccgc tgtggtcgga ccggcgctcg gcggagttcc gggttgaggc
     8401 gaacaacccc ctcaactcgg taagcttcag cggtttcggg acaacggtga acgcctccga
     8461 ctatggacgg cctaccgccg ccgcttccat gcgtaaagtg actgcgcagt tgcgcttccg
     8521 gttctgacca tgagaaggct gacactcatc gcgctcgtct cactcctggc tgcaggccag
     8581 actccgaagg agccagccag attccaagtg acaacgcgac tggtagtggt gaacgtcgag
     8641 gtcaaggacc gcaatggaag gccggccgag aacttgacgc gagacgattt cgagttaact
     8701 gaagacggca aggctcaaaa gatcgctgtt tttgactacc agcggcttga gaccaagccg
     8761 ggcacaactt cgcctgcggt cggggcgcgc cccgggaagt cgcgcaacga gataactccc
     8821 tcaaccccag gggaattccg ctatcgggac caccgcctgc tggtactcta cttcgatctc
     8881 tcctcgatgc cggcggagga ccagatccgc gcgcgaacgg ccgccgagaa attcattcgc
     8941 gaggagatga cgcctgtgga cctggtggcc atcatgagct tctcgagtga gctgaaggtc
     9001 ctgcaggatt ttaccgacga ccgagacagg ctgatgcaga cccttgctga gctccgcatt
     9061 ggcgagacga gcgacttggc ggggcaggcg gccacagggg agacggagga atcccaggat
     9121 acgggcgccg ccttcagcgc tgacgagact gagttcaaca tcttcaacac ggaccgtaaa
     9181 ctaagtgcgc tggaatcagc cacccggatg ttggcgtcct tgcctgagaa gaaggggctc
     9241 gtgtactttt cgagcggagt cggaaggacc ggtgtcgaga atcagtccca gcttcgcgcg
     9301 actgtgaatg cagcggtccg ggcgaatgtc tccttctatt cggtggacgc ccgggggctc
     9361 gtggccgagg ctccattggg cgatgcccgc tctgggggga atcgcgggtc gagtatgtac
     9421 tccggcggcg ctcagcgggg gcgccgagac acgttcctta atcagcaaga gacgcttttc
     9481 acgctcgctg ccgacaccgg cggtaaggca ctgctggatt cgaacgacct cagcataggc
     9541 atcgtccagg ctcaaagcga catctccagt tactacacgc tcggttatta cagtacgaac
     9601 actgccgagg acggaaagta ccggagaatt ctggtccgtg tgaaggcagt cgagaaggct
     9661 aagcttgact accgctccgg ctatttcgcc gctaaacgct tcgaagactt cagcgcgtcg
     9721 gagagagagc gccagctcga agaagcgctg ctacttgagg acccgatcac cgacctgaac
     9781 cttgcgctcg aggtcgatta ctttcgtcgc gaccgggacc gctatatcgt tccgttagcg
     9841 gtgaaaatcc ccggttctca gattcgcctg gctcagaaga gcggcgccga gacgacgcga
     9901 ctggacttca tcggccaagt acgcaatatt cgcggctttc tgatggggac tgtgcgggat
     9961 cacatccagg tgacgctccg aggtgagaac agcgcgcagt tgagccgccg aaatctggaa
    10021 tacgacagcg ccttcacgct gccgcctggt gagtacgtgc tgaagttcct ggtgcgggaa
    10081 aacgagaccg ggaagatggg cacgttcgag actcggttcg aaattccaaa cttggactcc
    10141 gaaaagtcct atctgaagct cagttcggtc gtctggtcga accagcggga gccgatgtcc
    10201 gaggcggtcg ggggagtgaa gagggacaag aggctggtca agaacgatcc actggtacga
    10261 gacagggaga aactcattcc cagcatcacc aaagtcttcc gcaaggacca gacactgcga
    10321 gtctttttcg aggtttataa tcccggagtt gttaacggcg agaagacagc caatctgacg
    10381 gccaacgtca gcctgtaccg tgcacaggca aaggtcttcg aatctgaggc catccgggtc
    10441 agtgaagggc aggggaatcg atatttcttg ccggtggaat tcgaaatccc gctggccaac
    10501 cttgagaccg gccgctacgt ttgccagctc aacgtcattg acgaagcggg gcgcaagttt
    10561 gccttccgcc ggacgccgct catgcttttg cccgccgcgc cgctgtaggc gcgcgtaaac
    10621 cccccagcgc aatccgcgtc aaatatggtt acggaggtac ccatgcagta tctttgcggt
    10681 tcggcgaggc tgactcgacg ggctgtcctg acatttacgg ctgcggccag tgcgtcactg
    10741 tggaaccgcc tcttcgcggc aaattccgac ttttggaata ggaagaagcc ggaagactgg
    10801 tcgagcgacg aaattgagcg acttgttacg aagtcacctt gggcgaagga ggtgaccgcc
    10861 gagttcaacc agcccgagcg tggcggacga ggcggtggaa tgggcgtccc aggcggcggg
    10921 atgggcgggc caaccacccg ccgtggcgga atgggtatgc cgcccatcgg cggtggtggg
    10981 atgggcatac cacccttggg tggcggagga cgcgacggca ggccttcggg cggaatggaa
    11041 aggcggaagg tgacggttcg ctgggaaagc gctaaaccga taagggaggc cctgaaaaca
    11101 ccgctgccgg attcgcttgc cgaccgttac gtaatcagtg tcaacgggct gccgatgatg
    11161 cccgggagcg gccgtagacg ggaggatgaa gggagcgagg acacttcccg ggcgccacgc
    11221 aatatgctcg agcgtctgaa agagacgact ttcctccagc ccaagggcaa ggaatccgtc
    11281 cagcccggtg tcgtcgagcg gcaacccagc gcgggaggcg caagcctcct ttttggattc
    11341 tcgagggaga ccctggtcct ccaccctgaa gacaaggagg tgacattcac aatggccatg
    11401 gggccgctgg aggtcaagac gaaattcaag ctcaccgaca tgacgtaccg cgaggagctt
    11461 gcactgtaac gatacaagtg tcaagccgcc cttccggcta gcgatgtcaa gctatagagt
    11521 gggcctgccg ccggctcgat gaaccatcag aagctcagca gcaagtgcca gatgtagagg
    11581 taggcgaaac cctcattgct ctcccagaca ctgcggacga gcgacggatc gccgcacatg
    11641 ggtggcagtt ccttgagctt ctcttcccac ggaaggcaaa cgccgggccg gacgggcggc
    11701 gctgtcaccc acccagggat gccaggcgtg ggcgcctccc agcgtggctc tacccgcgcc
    11761 ttggtcgacg gcgaagacgg cgagcggtag cagccgtact cgagcgttgc gtcggtcatt
    11821 gccttcaggt tctcgagcgt cgcgtcgttc tgcatgatgg cgctcgcatc cataatgtag
    11881 ccaccctcgg cgccgatggt ttcaatcagt ttgcggcatt gcgccttcac ctgttcgggc
    11941 ttgcccaacg tcaatagcgc gttcggaact ccgccgctga gacagaattt gcggccgagg
    12001 actcggtgaa cgcggtcagg gtcgccgcgg tcgatgtgaa agatgatgct tccggctggc
    12061 aattccgcga agcgctccag gtgggcgtcc cacttgcctt cggcataaaa gagcacctgg
    12121 ttgccctgag cccagagcgc ctcgacaatg ggtttgagcg tcggccagta gatcgtctcg
    12181 aagtgctcgc gcgaaatgaa cggcacgcag ccgcgatgca tccatatggg gatcgggacg
    12241 ctcttgccgg gatccgcgct gctgagcgca agatagccga ggtgcggcat cagcgcatgg
    12301 caggccgcca caaccttttc aggaatctcc tttaggtcga acgcaaggcc gatgtagccg
    12361 cgcagcttgt cggcgagaat gtcgagcggc gccttcagca tgccgcaaat cgaggagacg
    12421 gtgcccgttt cgcgccgcat tcgctcgacc tggggcccga aagccgcgaa gtagctcagc
    12481 atcgacatcc cgcccttcac gaaggaaagg ttgttgcggt aggtgaccgg cgtgcccttt
    12541 gcgctaacct cgcccgtgac acgcggcaac caggtttcgt acagaaatcg cgtcgggtct
    12601 tcgatcaggt cgtcgtactc ctcacgcttc atgaaggctt cccgctcgga tggctcgcgg
    12661 tactggaaac cgacgtcggg gccgacatcg atcccgggca cgccgtagta gcgcaggccg
    12721 atcgcctggg taaggccggt ccagacgtaa accatgttgg gaacgacggc gtcccagttg
    12781 tagtccttac agcaacggat gaccgcctca aacgccttgt tgtagtcgtg cgtgacctcc
    12841 tggcaggtga aacccgcgtg ccgggcggtg atctcggcgg cgaagggacg aagcgggaca
    12901 cgatccggca tcccgttgcg catggcggtg acgtagcggt tcagtcgctc ggagtacagt
    12961 tgctcgaagt cggttctcat tctccgtgac attgtacgcc gggagcgcca atggatggcc
    13021 gcactgctcc cagagggtcg cggagacggc gtgtcaggcg actgtggcca gccgcggctg
    13081 gtacacgcag ttggggtcgg attcgaggta gtttcccgtc agcgcgtagg cgcgggagcg
    13141 ggagccgccg cagagattcc ggtattcgca gtctccgcac ttgcctaaaa ggttgtcgct
    13201 gttgcgcagg gagcggaaga gcgccgagtt acggtaaact tcggcgatcg agtcccggcg
    13261 cacattgccc gcggccagtg gcaggaatcc gctcgggaaa atctcgccgg tgtgggacac
    13321 gaagacgaat cccttgccgt cgttgatgcc cgcctgccgc tggataacgt ccccagccgt
    13381 tgagcggtta gcggcgccac cctccgcctt ccgcctctgt gcgacatagc ggcgatagtg
    13441 ctgtgcctcg gtggttttga tgtcgaatgg cgcggttttc gaaagctcgt agaggaaccg
    13501 gaacacgtct tcgtactctt ccggcgttag atcgtcggag cgcatcgcgc ggccggtgac
    13561 aaccaggaag aacacgctcc agaggcgtgc atcccagtgg gccaccagtg tggcgatttc
    13621 ttcgagttgg ccaaaattat ggcgggtaac ggtggtgttg atctgtgtcg ggagtccagc
    13681 ccgccgggcg tgctcgagcg cgaagatcgt ccgctcgaac gagccgtcca ctcgccggaa
    13741 gccgtcatgc aaggcggcgt cgggaccgtc gagactgacg gccatgcggg ccacgccgca
    13801 gtcacggaag ccctgaatcg cttccgcggt gaggagcggc gtcgcgctcg gcgtcacggt
    13861 ggtgcgcaga ccgagtgaga cgctgtgccg taacaggtcg aaaaggtcgg gccgtttaag
    13921 aggatcgccg cccgtgaaca ccatcagggg atcgccgaaa gtgcggatct cttcgagcag
    13981 acggaaaccc tcgttggttg cgagttccaa cgggttgcgg caggcgatag ccgatgcgcg
    14041 gcagtgaagg caggccaggt cgcaagcctg ggtgacctcc cagatcacca cgaagggcgc
    14101 ctcgtcgaaa ctcctcatgc tcccagccta agcgcatttc cttcagtctc ctgtgacgag
    14161 tgtcacatcc tggcggggag atcgccccta gcattgggaa ggagacgcag gagggcgtta
    14221 tggaccttgc agagaacaag acggctgcta cggtgccggg ggaaggggcc gactggagct
    14281 cggccccgct gcactcgctg atggatcaca tcgtgggcat tcatcacgag tacctaaagc
    14341 tcgaattgcc gcggctggcc gacaaagccg gccggttggt gaaatcccgc atcgagaaat
    14401 acggtgactc ctctctcgtt ccgctcctcg acacgttcct agagctgaag agcgaactgg
    14461 agagccacat gtggaaggaa gaggttgttc tgttcccgct tatccggaat atggaggctg
    14521 ccgccaccgc gggagtcaaa gcgccgccgg cgcactgcgg ctcggtgaac aacccgatcc
    14581 gggtgatgga gcacgagcac gcctccgccg agcgggcgct ggaggagatg cggcgcatca
    14641 ctagcaatta cgccgttcct gagggagcgt gcgaaaccca ccggatgctg ttcgaggctc
    14701 tgcgcgtttt agaggaagac ctgtgcaggc acatccggct ggaaaacgat atcctgcacc
    14761 cgcgcgcgtc ggaactggaa atgctggtga gctgacgcgc ggagcctcga aacgatagag
    14821 cggacgccta cgttcgcggc cgg
//
LOCUS       DATIFQ010000089         2506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1559902, whole genome shotgun sequence.
ACCESSION   DATIFQ010000089 DATIFQ010000000
VERSION     DATIFQ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559902"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VLE22_03970"
     CDS             complement(<1..769)
                     /locus_tag="VLE22_03970"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HSB13589.1"
                     /translation="MFRILMVVVFTALLNAAPLTLVRDGRSSYSICISRDASPSEKRG
                     AAELQRFVEEMSGARLPIVTDAGNPRGALVLLGRSAVTDGLKLGIPYESLGPEGFALK
                     TAGEHLVIAGGRQRGTMYGVYTFLEKLGCRWFARDASRIPKLRTIVVPALDETQKPAF
                     EYREPFFTEAFDKDWAARNKTNGDHSELDDSTGGKVKYFPFVHSFYAMIPPEKHFQEH
                     PEYFSLIDGKRRVERGQLCLTNPDVLRLGIEAVERWIR"
     gene            875..1390
                     /locus_tag="VLE22_03975"
     CDS             875..1390
                     /locus_tag="VLE22_03975"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB13590.1"
                     /translation="MQTDQAVFLLNVLLPTVESEAETTKKVIAAIPEGKGDYRPDPKS
                     KTADELAWHIVSAEPWFLEGIAAGKFAEGDPGRPAELKTPADLVAWYEKNLAAGIEKV
                     KAMPPEKLAETLSFYGIFNYPAVTYLSFLTLHSVHHRGQLSAYIRPMGGKVPSIYGGS
                     ADEPFQMPEGA"
     gene            complement(1526..2290)
                     /locus_tag="VLE22_03980"
     CDS             complement(1526..2290)
                     /locus_tag="VLE22_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB13591.1"
                     /translation="MADTVCAKCGGSGWIIVEQDGISGAERCDCVAAERAGRIEDNAG
                     IPPLYRNASFDNFALPQDNPIANRGLAAVLNTVRAYTREYPVLPKPGLLFIGDPGTGK
                     THLAVAALRKLIARGFEGLFYDYQNLFDRIRASYDATSGAADKEAYRSALDAEILLLD
                     DLGAHRVTDWVEDTITSIVTYRCNHRRALIATTNLPDPDAGDALVERSADVQGVRYRT
                     SLADRIGSRARSRLFEMCKVVHMPLVEDYRLRKTVR"
     gene            complement(2309..>2506)
                     /locus_tag="VLE22_03985"
     CDS             complement(2309..>2506)
                     /locus_tag="VLE22_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HSB13592.1"
                     /translation="APMPGKVVRVLAAEGDLVEAGQGLLVVEAMKMQNEVKAPKAGRV
                     AALAAREGGTVAAGDVLAVIE"
BASE COUNT          472 a          868 c          713 g          453 t
ORIGIN      
        1 cccggatcca tcgctccacg gcctcgatcc ccagacgcag cacatccggg ttcgtcaggc
       61 acaactgccc acgctccacc cttcgctttc cgtcgattag cgagaagtac tcggggtgct
      121 cctggaagtg cttctccggc gggatcatcg catagaagct gtgaacgaac gggaagtact
      181 tcaccttccc accggtcgag tcgtccagct ccgagtgatc gccgttcgtc ttgttccggg
      241 ccgcccagtc cttgtcgaag gcctccgtaa agaacggctc gcggtattcg aatgcgggct
      301 tctgcgtctc gtcgagcgcc ggcacgacaa tcgtgcgcag tttcggaatc cggctggcat
      361 cgcgcgcgaa ccagcggcag ccaagctttt cgagaaacgt gtacacgccg tacatcgtgc
      421 cgcgctggcg tcccccggca atcacgagat gttctccggc ggtcttcagc gcaaagccct
      481 ccgggccgag cgactcgtac gggatgccga gcttcaggcc gtcggtgacc gcgctgcggc
      541 ccaacagcac cagcgctccg cgcggattgc cggcgtccgt aacgataggc agccgagcgc
      601 ccgacatctc ctcgacgaac cgctgcaact ccgccgcgcc gcgcttctcc gacggcgacg
      661 catcccgcga gatacaaatt gaataagaag acctcccgtc gcggacgagc gtcagcgggg
      721 ccgcattcag cagcgccgtg aagacgacca ccatcaggat tcgaaacatg agattccttt
      781 ctcccgtttt cccgttcctg tgaattatgc ctcgaaaccg cgcttgaccg cacactgcgc
      841 acgtgggaaa atcggccaat ctctgaggaa tctcatgcaa accgaccaag cagtttttct
      901 attgaacgtc cttctcccca ccgtggagag cgaagcggaa actaccaaga aggtgatcgc
      961 cgccattccc gagggaaaag gggactatcg tcccgatccc aaatcgaaaa ccgccgacga
     1021 actggcctgg cacatcgtat ccgccgagcc ctggtttctc gagggaatcg cagccggcaa
     1081 gtttgccgag ggggaccccg gccgtcccgc cgaactcaag acgccggccg accttgtggc
     1141 ctggtacgag aaaaacctcg ccgccggcat cgaaaaggtc aaggcgatgc cgcccgaaaa
     1201 gctggccgag accctgtcgt tctacgggat tttcaactat ccggcggtga cgtatctgag
     1261 ttttctcacg ctgcactccg tgcaccatcg cggccaactc tccgcctaca tccggccgat
     1321 gggaggcaag gtgccgagca tttacggcgg cagcgccgac gagccgtttc agatgccgga
     1381 aggggcgtaa cgaagacggc tgttagttta ggagcttaaa aacggtgaca ggctacgctg
     1441 ccacgcgcaa gaaacgcttg ggacagcgaa gccagccacc gttttcccag acaacgcttg
     1501 ggtcagcgta gccagccacc gttgctcacc tgaccgtctt cctcaggcgg tagtcttcca
     1561 ccagcggcat gtggacgact ttgcacattt cgaacagccg cgagcgcgcc cgtgagccga
     1621 tccggtccgc cagagacgtg cgatacctca ccccctgcac atcagcggag cgttccacca
     1681 gcgcgtcgcc agcgtccgga tccggcaggt tcgtcgtcgc gatcagcgcc ctccggtggt
     1741 tacagcggta ggtcacgatc gacgtgatcg tgtcctccac ccagtccgtc acgcggtgcg
     1801 cgccgaggtc gtcgagtaag aggatctcgg cgtccaacgc cgaccggtag gcttctttgt
     1861 ccgccgcgcc cgatgtggcg tcgtagctcg cgcggatgcg gtcaaaaaga ttctgatagt
     1921 cgtagaagag cccttcgaac ccccgggcga tcagtttccg cagcgcggcc accgcgaggt
     1981 gtgtctttcc ggtcccggga tcgccgatga acagcagtcc cggcttcggc agcaccggat
     2041 attcccgggt gtacgcgcgc accgtgttca gcaccgcggc aagcccgcgg ttcgcgatgg
     2101 gattgtcctg cggcagcgcg aaattgtcga aggaagcgtt ccggtagagc ggcggaatcc
     2161 ctgcgttgtc ctcgatcctt ccggcacgct ccgcggctac gcagtcgcag cgctccgccc
     2221 cggaaatgcc gtcctgttcg acgataatcc agccgctgcc tccacacttc gcgcacaccg
     2281 tatcggccat gttgagcact attatccgct actcgataac cgcgagcaca tcgccggccg
     2341 cgaccgtccc gccctcccgc gccgccagcg ccgccacccg tcccgccttc ggtgctttca
     2401 cttcgttctg catcttcatc gcttccacaa ccagcaggcc ctggccggct tccaccagat
     2461 ccccctcggc cgccagcacg cgcacaacct tacccggcat cggcgc
//
LOCUS       DATIFQ010000090         7175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_219902, whole genome shotgun sequence.
ACCESSION   DATIFQ010000090 DATIFQ010000000
VERSION     DATIFQ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219902"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VLE22_03990"
     CDS             complement(<1..325)
                     /locus_tag="VLE22_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13593.1"
                     /translation="MALLVGAVLAFAVGLLARGCDLDRDRAFYPTVMIVIASYYVLFA
                     VMEASTHVLVLESLVGAIFLALAVSGFRSSLWVVVVALAAHGIFDLVHGQAISNPGVP
                     GWWPQF"
     gene            complement(442..936)
                     /locus_tag="VLE22_03995"
     CDS             complement(442..936)
                     /locus_tag="VLE22_03995"
                     /inference="COORDINATES: protein motif:HMM:NF028012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HSB13594.1"
                     /translation="MTDVDDFIALVREYLHLVERAGGTQAHALLQACARVLPRIYATG
                     LALPNVEPGNDDAAPVVVSPLARLRSFLGRYDSYWEVFDPYVDEAPVVGSLADDLAGI
                     YLDLATPLAAFDAGRTADAVWEWSFNLRGHCGDHVVDAMRAIHRAVNDHMPPEYVAGS
                     DTAG"
     gene            complement(1087..1830)
                     /locus_tag="VLE22_04000"
     CDS             complement(1087..1830)
                     /locus_tag="VLE22_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13595.1"
                     /translation="MSSAHPPIWKSVIVASLVCFGIQGCGVLLWSNVKPVPIDEVDAH
                     GISLDRVQKLRRGDRAESVGLVLGEPADRQPSCLPGEVIWRYPIRAWNDMASRREIVP
                     AVLLRISFDGSDTLTDWEFIDPRTGHSLPVLENSDEASHWFQSLSHAPPPIPPRVEFD
                     EVLIRGRTTQLEVERILGQWRPDLHCGYGGPVPVVRKGTTDSGSVWDWYADRPSPLFV
                     PPRYLVVSFDDRGALIVWHFEQTYPGGRK"
     gene            complement(1952..2218)
                     /locus_tag="VLE22_04005"
     CDS             complement(1952..2218)
                     /locus_tag="VLE22_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13596.1"
                     /translation="MPILRDFRGALKMGFSESDDVVLLMVTFLVYAWCVADDLQVVPE
                     QRDVIMQTSHHGEAIVCCSNPGRLEEFVSSMVERGFPPHREKNA"
     gene            complement(2660..2962)
                     /locus_tag="VLE22_04010"
     CDS             complement(2660..2962)
                     /locus_tag="VLE22_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HSB13597.1"
                     /translation="MARPNEFSLATQQLALARQKFRCASCGTHISGLGQAGRADHRYG
                     EGAQAHHVRHVKLGGRDSLDNCVILCQACHYSAHEGGNYRFGTVTGRETDYPYFRG"
     gene            complement(3223..4161)
                     /locus_tag="VLE22_04015"
     CDS             complement(3223..4161)
                     /locus_tag="VLE22_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HSB13598.1"
                     /translation="MARIFFAYDARDRDLARGLEKGLEAKGHQSVWGVDELIAGRGWG
                     ELMPRRLASANAVVAILTPNSERSPSVWCEIGAARALANSEKRTALLPVIIGLDQAPS
                     YTKDTLVLWANSSEGNDKPTLVAEIDTAIKAHLAAIDAEAARVSSPKIFISHRHKDQK
                     IAAALTETIRTAFEVQQSEIRCTSVQPYRLPFGKNTGERLRDEIARAQVVLGILTPDT
                     TESSYVMFELGAAWSQRIYTCPLLSKGAEYNDIPGPIFDLAPARLWRASDCHQLLDNL
                     EAELHLTRRKDTQGAVSDRITALTEAASVAQGPAGA"
     gene            complement(4286..4951)
                     /locus_tag="VLE22_04020"
     CDS             complement(4286..4951)
                     /locus_tag="VLE22_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13599.1"
                     /translation="MDRDLVNAIHFMYRHGPLDWNYIWCSPDTKPDIEKFFDPTSPGT
                     LPNQLIAFLKAVEEQMSTLPKPDFAFGYPLKVPVRKLGDRRPCYAVMPYSMPWSLPVK
                     TAIITAAANCGFDCEVTGDLPTPGKIVDQIWQGIRKADAVIVDITGNNPNVFYELGLA
                     HALGKEVILLAQGSQDLPFDIKTTRYLTYDPANLKDLERSIENALKLVSARYPHEGPE
                     PRF"
     gene            complement(5073..6191)
                     /locus_tag="VLE22_04025"
     CDS             complement(5073..6191)
                     /locus_tag="VLE22_04025"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HSB13600.1"
                     /translation="MSTPSASDPVIQELTSLDAEIVPVLNDRASASRLLELLPSKYSP
                     STVVGPNGARVWELAALWFLNSNRVHEALGLFWRLYQQMVEGQQAAWAHKGMPLVWLS
                     ECYARLGFALHAKRYLMLTLCEDALRGNGSVSPNTSGVYFRLVWRQGLSDYELRRYAA
                     QFFWLSQQMPEEAKYPEALLQRVDDRWLTEAPAPAESGSYVVNAAYVRHLLGKLRDKS
                     GHTLELLAEYLMASMPGCRAKRRQRSGSTDYDVVCSMEGFDVDFRSELGRYFVCECKD
                     WSSPADFTVMAKFCRVLDSTKSRFGVLFSRSGISGTGETAFAALEQLKVFQDRGVIIV
                     VLDLEDLEAVASGANLTQLLREKYEAVRLDLRGSGHAG"
     gene            complement(6458..6694)
                     /locus_tag="VLE22_04030"
     CDS             complement(6458..6694)
                     /locus_tag="VLE22_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13601.1"
                     /translation="MKSSRLRLGGVLAFGLFAPIWWTWAVGNLTYLIYVWSGARERAT
                     KTLLWASIYLSSFALGLVAGAIVALVLVEAPLKG"
     gene            complement(6906..>7175)
                     /gene="tnpA"
                     /locus_tag="VLE22_04035"
     CDS             complement(6906..>7175)
                     /gene="tnpA"
                     /locus_tag="VLE22_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HSB13602.1"
                     /translation="IQVDHVHLVAVIPPKYAVSAIVAKIKANTSREIRQRFPWIKKVY
                     WRNEFWSVGFFSSPVGIDEAVIKRYVEFQEKIDRGQLKLQLDFGF"
BASE COUNT         1492 a         2199 c         2084 g         1400 t
ORIGIN      
        1 agaactgcgg ccaccaacct ggaacgcccg ggttggatat tgcttggcca tggacgagat
       61 cgaagatgcc atgtgccgca agcgcgacca cgaccaccca tagcgatgac ctgaaacccg
      121 ataccgcaag ggccagaaag attgcaccaa ccagcgactc cagtacgagc acgtgggtgg
      181 acgcctccat gactgcgaac aagacatagt aggacgcgat gacaatcatt actgttggat
      241 aaaaggcacg atcacgatcc aggtcgcaac ctctggccaa cagccccacc gcgaatgcca
      301 gaactgctcc gacgagtagg gccacgatgc gccttttgac ctgatctgca acctaacctg
      361 atggcgttca cccgcgggcg gcaagcgcag cgagccacgc tcgattggca tcctgccgcc
      421 ccccccgggt gcaacgccag gttatccggc tgtatcgctg ccggcgacgt attccggcgg
      481 catgtgatcg ttcacggctc gatggatcgc gcgcatggcg tcgaccacgt gatcgccgca
      541 gtgcccgcgg aggttgaagc tccactccca aacggcgtcg gcggtacggc cagcatcaaa
      601 agctgcgagt ggcgtcgcaa ggtccaggta tatgcccgcc aggtcgtcgg ccagcgaccc
      661 gacaacaggt gcttcatcga catatggatc gaaaacctcc cagtagctgt cgtaccgtcc
      721 gagaaaactc cggagccgcg ccagcggcga gacgacgacc ggtgctgcgt cgtcgtttcc
      781 cggttcgacg ttcggcaggg caagccccgt cgcgtagatg cgtggaagta cccgcgcgca
      841 ggcttggaga agagcatggg cttgggtacc acctgcccgt tcaacgaggt gaaggtactc
      901 cctgaccaac gcgatgaagt cgtcgacgtc cgtcatcttg tcgcctggtc ggacggttcg
      961 acgctcagct gcgagccact gcgcctctgc agctcgctcg aagcgcctga ctcagacgcc
     1021 agacggtatc acgggccgat cggagcgcgc tgtgcctcgg cagatggagc gtcttgttag
     1081 gccgcgtcat ttccttccgc ctgggtaggt ctgctcgaag tgccagacga tcaaggcgcc
     1141 tctgtcgtcg aaggagacga ctagatagcg cggcggaaca aacagggggc tgggccgatc
     1201 ggcgtaccaa tcccaaaccg agccagagtc ggtcgtgccc ttcctgacca ccggcacggg
     1261 acctccgtat ccgcaatgga ggtctggtcg ccactggccg agtatccgct cgacctcgag
     1321 ctgcgtggtt ctccctcgga tcagcacctc gtcgaactcg acacgcggtg ggatcggtgg
     1381 cggtgcgtgt gagagtgact ggaaccagtg ggacgcctca tctgagtttt ccaggaccgg
     1441 caaggaatgc ccggttcgag gatcgataaa ttcccaatct gtcagcgtat ccgacccgtc
     1501 aaagctgatg cggagaagga ctgccggaac gatttctcgg cggctggcca tgtcgttcca
     1561 agcccgaatg ggatatcgcc atatgacttc accgggcaag caacttggct gtcgatccgc
     1621 aggttcaccg agaacgagtc ctacggattc cgctcgatcg ccgcgcctga gcttctggac
     1681 acggtcaagg ctgataccgt gcgcatcgac ttcgtcgatc ggcacgggct tgacgttcga
     1741 ccacaggaga acgccacagc cctgaatgcc gaagcagacc agcgatgcga cgatgacgga
     1801 cttccagatc ggcgggtgag ctgacgacat tgtcatcacc cttcggggtg gaggggtgac
     1861 ttgggctccc atgacgcggc ccgacgacaa gcatcagccg ggcgcgaagc gcagcggctg
     1921 gatgcattgg ctaggcccac acctcgaaga gtcacgcgtt cttctctcgg tgaggtggaa
     1981 agccccgttc caccatcgag gacacgaact cttcgagtct cccgggattc gagcagcaga
     2041 cgatcgcttc tccatgatgg gatgtctgca tgatgacgtc tcgctgctca ggtacgacct
     2101 gcagatcatc ggcgacacac catgcataga ccagaaaggt caccatcagc aagaccacgt
     2161 cgtcactctc actgaaaccc atcttcaagg cgccacggaa gtctctcagg atgggcaacg
     2221 tgccgacaat tcaatcgcgc aggtgttccc actcgggccc cgtggcggga tcacagcccg
     2281 tgaaccaagg cgctccgccc ttgatgaaca agtgcacgga cgcctgctgc gctgtgcaac
     2341 cgagcaggca cgaggcaaac cccggcagat tgaatggtgt ctccggggcg attcaccgac
     2401 gcacggatgg tgaggtacca cgaaacgtta gcggagcttg cccgtcccgc tcacgtgggt
     2461 cgggcacgat gccgcctttc gcggcccacc gcaagaagtc attcgcggcg acaagctcca
     2521 tcaaaccctg cctgcgggaa gggtctaacg cccacgttgc cggccgcggc gagcgaaccc
     2581 gagccagcgg tccggtccaa cgtgctgtta tgtggcatgt ccggcacggg ggtttcggag
     2641 cattccctcc gctccaaggc tatccgcgaa aatacgggta atcagtctca cgcccggtga
     2701 ccgtgccgaa tcgatagttg ccgccctcgt gggcggagta gtgacaggcc tggcataaga
     2761 tgacgcagtt atccagggag tcccggccgc caagcttaac gtggcgcacg tgatgggctt
     2821 gcgctccttc gccgtaccgg tggtctgccc gcccagcctg gccaagaccc gagatatgcg
     2881 ttccgcagct tgcgcagcga aacttctgcc gggcaagggc tagttgctgg gtggctagag
     2941 aaaactcatt cggtcgcgcc atcgtcctgc cctccttcac ccaagaccgc tgcccgggca
     3001 ggctgtgtct tggcgcactg acttctgcac tgtcccgaag gccgcagaac acgatggtca
     3061 ccggcgtggt gcgccgttct gccacgtccg gtgcacctat ttgttgggcg gcgcggccat
     3121 gggttgcact tcggatctcg cagcgcgcgg gccacactgt ggttgagcgg attcagcacc
     3181 tcgtgatcgt acagacctta acctcatcgt cacttcagct cactaggcgc cggctgggcc
     3241 ctgcgcaacc gaggcggctt ccgtgagggc cgtgattctg tcggaaaccg ccccctgtgt
     3301 gtccttcctt cgggtcaaat gaagctccgc ctcgagattg tcgaggagct gatgacaatc
     3361 ggacgctctc caaaggcggg ccggcgcgag gtcgaagatc ggacccggta tgtcgttgta
     3421 ttcagctcct ttcgaaagaa gcggacacgt atagatgcgt tgcgaccagg cggcaccgag
     3481 ctcgaacatg acgtaggagg actcggttgt gtcaggcgtg aggattccga gaaccacctg
     3541 cgcccttgca atctcgtcgc gcaggcgctc acccgtgttc ttaccgaatg gaaggcgata
     3601 cggttgcaca gaggtgcaac ggatctcgct ctgctgcact tcaaatgccg tgcggatcgt
     3661 ctctgttagc gcagcggcta tcttctgatc cttatggcgg tggctaatga agatcttcgg
     3721 tgacgagact cgcgctgcct cggcgtcgat ggccgccaag tgcgccttaa tagctgtatc
     3781 gatttcggcg acgagagtgg gcttgtcgtt tccctctgaa ctattcgccc aaagcaccag
     3841 cgtatccttc gtatagcttg gcgcctggtc aagtccaatg atgaccggca gaagggcagt
     3901 ccgcttctcg ctgttcgcaa gcgcacgagc ggcgccgatc tcgcaccaca cggacgggga
     3961 tcgctcagag ttcggtgtca aaatcgcgac aaccgcattg gcgctcgcga gccggcgggg
     4021 catcagctcg ccccagcccc tacctgcaat tagttcgtcg acgccccaca cgctttgatg
     4081 gcccttcgcc tcaaggcctt tctctagacc tcgcgcaagg tcccggtcgc gtgcgtcgta
     4141 cgcaaagaag attcgcgcca ttgattctct cctctaatgc ggcgaggtcc gcaaaatcct
     4201 gcgatgcggc ttcgccgccc aacgggagcg tgagcgcgtg gctgaaagcc atccgttcca
     4261 cactttggtt agacggagtc atgtctcaaa atctcggctc gggaccttcg tgcggataac
     4321 gggccgagac caactttagg gcgttctcga tcgacctctc aagatccttg agattcgccg
     4381 ggtcgtaggt aagatatctc gtggtcttta tatcaaacgg caaatcctgt gatccttgag
     4441 ccaggaggat tacttcctta cccagcgcgt gcgccaaccc tagctcatag aagacattcg
     4501 ggttgttgcc cgtaatatcg acaattacgg cgtccgcctt gcgaatccct tgccagatct
     4561 ggtcaacaat cttacctggc gtaggcaaat ctcctgtcac ttcacaatca aaaccgcaat
     4621 tagcagctgc ggtgattatc gcggtcttaa cgggcaggga ccaaggcatg ctgtacggca
     4681 taacggcgta gcagggccga cggtcgccaa gtttgcgtac ggggaccttg agtggatatc
     4741 cgaatgcgaa gtcgggttta ggtagggtgc tcatttgctc ttcaactgcc ttcagaaacg
     4801 cgatgagctg attcgggagt gtaccaggac tggttgggtc gaaaaatttc tcgatatccg
     4861 gctttgtgtc agggctacac caaatataat tccaatcgag tgggccatgg cggtacataa
     4921 aatgaatggc attgacgagg tcacgatcca tagtgtttac ctccaacttt tgcccctatt
     4981 gtagtctgct tgttcttcag tcactggggg aactccgtct aacgctctcg cgatgagccg
     5041 cggcgccgtt cagccgccgg ctccatcgcg tgttagccag cgtgcccgga acctcgcaga
     5101 tcaagacgca cagcttcgta cttctcccgc agcagctgcg taagattcgc cccggaggct
     5161 actgcttcaa gatcctcgag atcaagtacg acgatgataa cgccgcgatc ctggaacacc
     5221 tttaattgct ccagagcggc aaatgcggtt tcccccgttc cggagattcc gctccttgaa
     5281 aacagaacac cgaaccgtga tttggtcgaa tcgagcaccc ggcagaactt ggccatcact
     5341 gtgaagtcgg caggcgagct ccagtccttg cactcgcaca cgaagtaacg tcctagctcc
     5401 gagcgaaaat cgacgtcgaa accctccatt gagcagacga cgtcgtagtc ggtcgatccg
     5461 ctgcgttgtc gacgtttcgc tctgcagcca ggcatggaag ccatcaagta ctcggcgagt
     5521 agctctagcg tgtgacctga tttatcgcgg agcttcccga gcaagtggcg tacgtacgcg
     5581 gcattcacga catagctgcc cgactcggct ggcgccggcg cctcggtcag ccagcggtca
     5641 tcgactctct ggagtagggc ctctgggtac ttcgcctcct cgggcatctg ctgcgacagc
     5701 cagaagaact gggcggcgta tcgtcgtagt tcgtagtccg ataacccttg ccgccagacc
     5761 aaacggaagt agacaccaga agtatttgga gagactgatc cattcccacg caacgcgtct
     5821 tcgcagagcg tgagcatgag gtagcgcttc gcgtggaggg caaacccaag tctcgcgtaa
     5881 cactcactca gccacacgag aggcatcccc ttgtgcgccc aggctgcctg ttgcccttca
     5941 accatctgct gatagagccg ccagaacagg ccgagtgcct cgtgcacccg atttgagttc
     6001 aggaaccaca gtgccgctag ctcccacacc ctggccccgt tcgggcccac gaccgtggac
     6061 ggcgagtact tcgacggtag gagttcaagc agtcgagagg ccgaagcgcg gtcgttcaga
     6121 accggcacta tctccgcgtc cagtgacgtg agttcctgaa tgaccggatc agatgctgat
     6181 ggcgtcgaca tgtctagagg gtgtccgact agctggctaa cgtccgcgtt gaacggcgcg
     6241 agcaagggag cacggcgacg cttgcgaacg tccgtcttcg aacgcgttgt tagccggcac
     6301 gcttcttacc ccatgcagcg acaggcaaaa ttgccgagcc gatggagaaa aaccagatat
     6361 ccacagactc aaacatcgtg taaatgtgtt cggatggcgc acccaagacc gcgcccaaga
     6421 caagcgcgcc gagcaccagt gaaccgaaaa acgccagcta tcccttcagc ggtgcctcca
     6481 ccagcacgag agcaacaatg gcccctgcta ccaaaccaag ggcgaatgac gacaaataga
     6541 tcgaagccca caacagcgtc ttcgtcgcgc gttcacgtgc gccgctccag acgtatatga
     6601 ggtaggtgag attgcccact gcccaagtcc accatattgg agcgaacagg ccgaacgcca
     6661 gtacaccgcc aagccttagc cgtgatgatt tcatgtgctg gctaactaca agtatgcaga
     6721 actgtatagt ctaccggaag ctgacaggct tgcgggctag cctgccgaga agatgcgtga
     6781 cctacgaacc ggaactcctc gtcgttgatc cggttgcggt aattccagaa ctccgccagc
     6841 gggctcgcta tgctggccgg cctccggcgt agatacccac gggcttacgc ccgtggcact
     6901 gcggatcaga acccgaagtc caactggagc ttcagttggc ccctgtcgat cttctcctgg
     6961 aactcgacgt agcgcttgat cactgcttca tcgatcccga caggtgacga gaagaagccg
     7021 acggaccaga attcattgcg ccagtacacc ttcttgatcc aggggaaccg ctggcggatc
     7081 tcccggctgg tgttcgcctt gatcttcgcg acaatcgcgg agaccgcgta cttgggcgga
     7141 atcaccgcca ccaggtgcac gtggtccact tggat
//
LOCUS       DATIFQ010000091        19792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_603100, whole genome shotgun sequence.
ACCESSION   DATIFQ010000091 DATIFQ010000000
VERSION     DATIFQ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19792
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603100"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1800
                     /locus_tag="VLE22_04040"
     CDS             211..1800
                     /locus_tag="VLE22_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB13603.1"
                     /translation="MPIYRVTVIERTTKAVNYHHRGGATKIDFQGTPLLPQARGEAKV
                     ESKQGYIEIEVEFDDLKPATGYGAEYLTYVLWAITPEGRTSNLGEVLLNGTKSKLNVT
                     TELQSFGMIMTAEPYFAVTQPSDVVVMENVIRQDTSGTIEEVAAKYELLQRGQYVFNV
                     NPAEIKPVRLDRKVPLELYEARNAVQIARWTGAQQYASETYQKAVEGLQNAEGYLTRK
                     AGTKPIVTAAREAVQMAEDARIITVKRIQEDKLANERQAGVDREMRAENDRAAAQSET
                     ERVAREAEKARVTAQAEAERLRQDNDARTAAAQNEADRLRRENDAQRAAAQADADRLK
                     RENEAQAAAAQNEADRLRRENDAQRATAQADLDRAARERAQAEAEKTALRAQLLAQFN
                     AILQTRDTARGLIVNMSDVLFDTAKFSLRPLAREKLAKVAGIVSGHPGLRLDVEGHTD
                     SVGGDDYNQRLSEQRGESVRGYLIQQGMPVSSVTTRGFGKTQPVASNDTAAGRQQNRR
                     VELVISGDIIGAAIGSPIAAK"
     gene            1957..2379
                     /locus_tag="VLE22_04045"
     CDS             1957..2379
                     /locus_tag="VLE22_04045"
                     /inference="COORDINATES: protein motif:HMM:NF042853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6804 family protein"
                     /protein_id="HSB13604.1"
                     /translation="MNTIGTVTNEKQGLSWRDWQERNHLQDLHRVALRAKLTKYASIV
                     VLLLAVVFWSYAADYQAVLGFVVCAGAVRVAFLAAAVRRYDWASLFIGMALLYNPVFP
                     LFALTGRVAFFLVIASITAFAASLFLLKPRLTPSVSGC"
     gene            2574..2747
                     /locus_tag="VLE22_04050"
     CDS             2574..2747
                     /locus_tag="VLE22_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13605.1"
                     /translation="MPELFESGLREIFPYLLKLECAWDFEGKTAWGLEQEIKDSIRER
                     PEKELTDDESPER"
     gene            complement(2850..3074)
                     /locus_tag="VLE22_04055"
     CDS             complement(2850..3074)
                     /locus_tag="VLE22_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13606.1"
                     /translation="MEPSFITVGEYRINPAYVSYIVLRTEELEGRFAGKQQEGAEIHF
                     VGVQEPLWLEGQQGQDFLLLYEKHLPRLSS"
     gene            complement(3605..4483)
                     /locus_tag="VLE22_04060"
     CDS             complement(3605..4483)
                     /locus_tag="VLE22_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSB13607.1"
                     /translation="MDLALVIRRRLKELGVGQRDLAAAAQVTDSYISQLLGGKKTPPA
                     PNRTDIYDKMGEFLRLPSGELARLADLQRREALKKKLAEPPRPLFQGFRELMLRKCAP
                     AKQDQVRAIFGKEPFGELERLVTQKLLDVAKRVAKEELESENWLRLVARLSGRTYEQM
                     RVTILEFLETDVFHVSVENWVSFLDPLIESWDVDLMTFGVEIVLNRRLALGPLKRFEF
                     VEREPEQPFEMEPGLEEFLKNSALSGDASEEEIEFLKRLKFKARHPAALYYYRELQNL
                     RDPLHFPTPQSEGGGT"
     gene            4682..5008
                     /locus_tag="VLE22_04065"
     CDS             4682..5008
                     /locus_tag="VLE22_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13608.1"
                     /translation="MFARTVSIRLKPNGIVEFSRVIENETIPLLRKQKGFQDEITFFI
                     PGGSEAVEISLWDRREDADAYGRKATAKLRKAFENVIEGDSEVRTYAVSNSTFHKIAV
                     PVFARS"
     gene            5093..5458
                     /locus_tag="VLE22_04070"
     CDS             5093..5458
                     /locus_tag="VLE22_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13609.1"
                     /translation="MLTKTMKWITIGALVAAAILWRSAANSQVPQFLLGFVVCLGASV
                     VVLQAGRVKKFVWAGGFTAIALLFNPLVPVFPFGGEWGRWLVLLTLVPFAVSLITLRT
                     QPLLSIPSITDRNPGSESL"
     gene            5518..6201
                     /locus_tag="VLE22_04075"
     CDS             5518..6201
                     /locus_tag="VLE22_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13610.1"
                     /translation="MTSFRIFAVSLSWVVLASPLLYAGDLSRYRSFQLGADLPTIAGQ
                     ARMDPSEAKVVHRLPALIQVLEWRPQPLGPSTRVESADKVVFSFYNGELYRIVVNYDR
                     YNTEGLTAEDLIEAISATYGTATTNAAGITLQSVYDANENVKVIACWEDSSYSMNLVR
                     SSYQPTFALVVFSKRLDSLAGVAVAEAVRLEKEQAPQREIERQEKQAEENRVLQEKAR
                     LANKPGFRP"
     gene            6228..6662
                     /locus_tag="VLE22_04080"
     CDS             6228..6662
                     /locus_tag="VLE22_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB13611.1"
                     /translation="MTDTIKTGTLLFVGAAEGDGLSLGAILRESDWQLHGTRNCQEAL
                     EFLDKNRISVVLSEAELPDGNWRELLNSMAKRSAAPSLIVSSRLADDRLWAEVLSLGG
                     FDVLMTPFETEEVLRVTFGARQNSRSRAEGLQRLGQPGGRNE"
     gene            6655..7491
                     /locus_tag="VLE22_04085"
     CDS             6655..7491
                     /locus_tag="VLE22_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HSB13612.1"
                     /translation="MSSVLSINVTTITIIIVAIYLLSSIKILKEYERGVIFRLGRVLP
                     RAKGPGIILVFAPIDRMTRISLRIEALEVPAQDVVTRDNVTVKVNAVIFFRVIDPRLA
                     VLEVSNFLYATSQLAQTTLRSVLGEAELDELLSQRGKLNIRLQSILDQHTSSWGMKVT
                     LVEVKQVELAEQMIRAIARQAEAERERRAKVIHAEGEYMAAEKLGMAAEVIQRQPAAI
                     QLRYLQTLVEIGTEKNTTVVFPLPIDMISSLGRLLDKFAGPGRSTVSESRGDTVGSIE
                     SH"
     gene            7505..7720
                     /locus_tag="VLE22_04090"
     CDS             7505..7720
                     /locus_tag="VLE22_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13613.1"
                     /translation="MFNAHPARTTKFKESDNVVLAEGTYQGATGVFIRLRPDVNWAEI
                     RERNGTVRCHPVVWLQHADPPGKNVSG"
     gene            7791..8336
                     /locus_tag="VLE22_04095"
     CDS             7791..8336
                     /locus_tag="VLE22_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13614.1"
                     /translation="MTDTIKTGTILIEEGTPVPDSLLLESEPYSSGWGSLRNLDLRGL
                     DRELTKTGWTFFFMAGEIRATAFGFDRRRAVREAVQRIIASVKSQKCNCLEISQVAMK
                     SFLRVPYVTVSAHARHIQEGVVFLRPRQRDRTVRLPAADPRIRRQRSEITEEPLFTEE
                     AVAAWEDEGGAPGHMGARLAA"
     gene            8387..8698
                     /gene="groES"
                     /locus_tag="VLE22_04100"
     CDS             8387..8698
                     /gene="groES"
                     /locus_tag="VLE22_04100"
                     /inference="COORDINATES: protein motif:HMM:NF001533.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSB13615.1"
                     /translation="MKIRPLYDRIVVKRIEEQETVKGGIIIPDTAKEKPQEGEVVAVG
                     KGKRLEDGKMVPLDVQVGDRILFGKYSGSDIELGGDEYLIMREDEVLGIIGKTPKKTG
                     A"
     gene            8717..10339
                     /gene="groL"
                     /locus_tag="VLE22_04105"
     CDS             8717..10339
                     /gene="groL"
                     /locus_tag="VLE22_04105"
                     /inference="COORDINATES: protein motif:HMM:NF009488.0"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSB13616.1"
                     /translation="MAAKLIIYSENSRQAILRGVNQLADVVKVTLGPKGRNVVLDKKF
                     GGPAITKDGVTVAKEIELKDPLENMGAQMVREVASKTSDVAGDGTTTATILAQSIFRQ
                     GVKSVAAGANPMAMKRGIERAVQAVVEQIKKYSKPVSGAMIAQVGTISANGDTTIGDR
                     IAESMKKVGKDGVITVEESKTMETTTDMVEGMQFDRGYLSPYFVTDAERMECVLEDPY
                     ILIHEKKISNMKDLLPLLEQVARSGKPMLVVSEDVEGEALATLVVNKLRGTLNACAVK
                     APGFGDRRKAMLEDIGILTGGKTIMEETGIKLEGVRLDDLGRAKRVTIDKDNTTIVEG
                     AGSHNAIEGRIKQLRAQIEETTSDYDREKLQERLAKLAGGVALIKVGAATETEMKEKK
                     ARVEDALHATRAAVEEGIVPGGGVALFRSAGALEMLKVESEEQIGVDIVKRACEEPLR
                     QICQNAGFEGAVVAEKVRVNDNVNFGFNAETEQYEDLVKSGVIDPTKVTRTALQNAAS
                     IASLMLTTEAMICEIPEKKEPMPGGHGPDMDY"
     gene            11067..11498
                     /locus_tag="VLE22_04110"
     CDS             11067..11498
                     /locus_tag="VLE22_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSB13617.1"
                     /translation="MELARFNAYNDFPTGMQLFHDAINRLLNDENQVRPWSPAVDIFE
                     TETELFFKVDAAGMSLEDIDVQLENGTMTLKGERKFEKDDKVKGYHRIERSYGAFARS
                     FTLPNTVDPEGVRADYTHGVLTITLPKKELAKPRAIKVNVN"
     gene            complement(12129..12278)
                     /locus_tag="VLE22_04115"
     CDS             complement(12129..12278)
                     /locus_tag="VLE22_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13618.1"
                     /translation="MGLRWTIGEGQPTGSVKITANPYDDGNKQSYLRVNYTLPGRSTL
                     IVVSQ"
     gene            complement(12773..13849)
                     /locus_tag="VLE22_04120"
     CDS             complement(12773..13849)
                     /locus_tag="VLE22_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HSB13619.1"
                     /translation="MQICAALVTVCIAFAQGVSSTRQSPVPEFPRRVILDTDPGIDDA
                     MAVLLALRSPELKVEAITTVAGNVPVELGTENARKLVELARRTDVIVAKGAARPLQRK
                     LSTAEAIHGENGLGGVVLPKPNIGLDPRHAVQVIHDLIEANPGQITLVPVGPLTNIAM
                     AFLQYPDLAAKTREIILMGGTVGAGNVTPAAEANIYHDAEAAKIVFESGVPLLMVDLT
                     ACAQARLTRNDAAQLRGSTDPVARFVGAIAEPYLSFSERFGPGGAAIHDALAVGIAID
                     PQVARTVKPVHVDIETKGEFAYGATVTNQLLTAEHSEWRGDRFVIVGFPRVPANAAYP
                     AVVDGERFVRMFSERMMRAPARRN"
     gene            complement(14202..15518)
                     /locus_tag="VLE22_04125"
     CDS             complement(14202..15518)
                     /locus_tag="VLE22_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB13620.1"
                     /translation="MAGREVYEFGEFTLDVGERRLTKHGQAIGLAPKAYDVLVVLVRN
                     GGRLVRKDELLAAVWPESFVEEGILAVHISHLRKALGRRQYIETVARSGYRWVSGVGM
                     RPLLPVHTSEVYELIGRGRAHLLAASMTETPKAVAAFQAAVALDPTYAAAHAGLALAW
                     CAQAEFRVMPHAEAYHQAKAAALAALALDASCADAQVALGIVLFLSEWNWIGAERSLR
                     RALELNPNHTEAYLVCGRLLEAIGKLEEGLQLKQKALERDPFSPLVHLQISLSYWNQR
                     RYDESIEWANKALELDPRHLLAREHLAGAYWKKGDYDRHMAEAIRHAEDCGAPGELLD
                     ELKRAYAEGGRAAVVTYSLSRMASGGDLVPAIQLAVLYGEAGDLDAAFHHLGRALEGR
                     DPCLVHLAVAPQWDCLRADPRFNNCLARMGLPQPTVAMAEHKFVIR"
     gene            15669..16079
                     /locus_tag="VLE22_04130"
     CDS             15669..16079
                     /locus_tag="VLE22_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13621.1"
                     /translation="MPNQQDPFSGTWKMNPQKSNFDPNHRPSEATMRFERESEGYLMR
                     AEGVCDGKHVEEQPQRFILDGREHPVPGVLDVTAVSTRPDPNTIRAAARSGDRTVGEG
                     SYVVSADGATLTATVRGIDAQQRPFQTTVVWDRQ"
     gene            complement(17079..17957)
                     /locus_tag="VLE22_04135"
     CDS             complement(17079..17957)
                     /locus_tag="VLE22_04135"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HSB13622.1"
                     /translation="MHLLAIALLAQATMATAAELRWPVVLPMLMEKNLPLVQVTVNGA
                     QTLTFILDSAAANCVVDRARAAALGLNESGSAISSGSGGAQQVGLLVGVHLNLGELEI
                     RPPRSITFDMKSLAFQSPVAGILGFPLFGKYIVEIDYPGSRVRIFQPGSYQPSPTADM
                     LPIRMTIGPVVRGSIRVRGQRPIEADMQLDTGSAHVLTICSPFVDRHQLLDSADEVAP
                     GTTRGFGGATVDMVGRIEEVRIGRFAADKPVVRFSRQTTGTLGSEQHYSANLGGEFMK
                     NYKVTFDIPGSRLFLE"
     gene            18728..19351
                     /locus_tag="VLE22_04140"
     CDS             18728..19351
                     /locus_tag="VLE22_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13623.1"
                     /translation="MNRAYAVVACLTLVIAAPPPTSAQTNLGTRVTIDVTEVAPQKVF
                     ELLARSLDCTITVDPAVKKPLTLRVVDMPATDILSMVCQSIKCEYRFDGKNIFVKPLS
                     ASRRRQMALMEDHSRKLMSRLPTGMHFDGTPLKDVLDTIAKAAGLKLRPWKDEGGRKV
                     TIDVGGKTVNEALEAVVRQIDGEGVVMMRTWAGSWGQYRLVDKRREP"
BASE COUNT         4200 a         5787 c         6007 g         3798 t
ORIGIN      
        1 caactgaacc gtatttcctc gcgggaaggc aaagaagttg gccagacgtc gaaatcacga
       61 agtgcaaaga agaactgagg cagaaaaatg aaaatgatca ctgcattact gaccgtatgc
      121 gggtgtctgg cggcccaggc gccgcaagct ccgaacccga ctcaacgatc gactccaacc
      181 gagaatgagg caaggatggc cgctgggtcg atgcccatct atcgcgtcac cgtgattgag
      241 cggactacaa aagcagtcaa ttaccatcac cgcggtggag ctacaaagat cgatttccaa
      301 ggcacccccc ttctgccgca ggcccgcggc gaggccaagg tggaaagcaa acagggttac
      361 atcgagatcg aagtggaatt tgatgatctt aagcctgcga ccgggtacgg ggcagagtac
      421 ctcacgtacg tgttgtgggc catcaccccg gaaggccgca catccaacct cggcgaagta
      481 ctcctgaacg gaaccaaaag caagctgaac gtgactaccg agctgcagtc gttcggcatg
      541 atcatgacgg cggaaccgta tttcgcggtc acacaaccca gcgatgtcgt ggtgatggaa
      601 aacgtgatca ggcaagatac gagcgggact attgaagagg tcgccgcgaa gtacgaactg
      661 ctgcagaggg ggcagtacgt tttcaacgtg aacccggccg aaatcaagcc tgtgcgactg
      721 gatcggaagg tgccattgga gctctacgaa gcgcgaaacg ccgttcagat tgcacgttgg
      781 acgggcgctc aacagtatgc ttctgagacc taccaaaagg ccgtcgaggg gcttcagaac
      841 gcggagggct atttgacgcg taaggcgggc acgaaaccga ttgtgacggc ggcgcgcgag
      901 gccgttcaaa tggcagaaga tgcccgaatt atcactgtca agaggataca ggaagacaag
      961 ttggcgaacg agcgccaggc gggagtggac cgcgaaatgc gcgcggagaa cgaccgggct
     1021 gccgcgcagt ctgagaccga gcgagtcgca cgggaagcgg agaaagcgcg ggtcaccgca
     1081 caggccgaag ctgagcgctt aaggcaggac aatgacgcgc gaactgcggc cgcgcagaat
     1141 gaagctgatc gtctgagacg ggaaaacgac gcccaaagag ctgctgccca ggcagacgct
     1201 gatcgtttga agcgggaaaa cgaggcccaa gcagcggctg cgcagaatga agctgatcgt
     1261 ttgagacggg aaaacgacgc ccaaagagct actgcccagg cggacctgga tcgcgcggcc
     1321 agagagaggg cacaagcgga ggctgaaaaa acggccctgc gagcccagct attggcacaa
     1381 ttcaacgcga ttctccagac tcgggataca gcccgcggct tgatcgtaaa tatgtcggat
     1441 gtccttttcg acacggctaa gttttcgctg cgccctctgg cgcgtgagaa gctggccaag
     1501 gtggccggca tcgtctcggg gcacccgggt ctgagactgg acgtagaagg tcataccgac
     1561 agcgtgggcg gcgatgacta caatcagcgg ctctccgaac aacgcggcga gtcagtgcgc
     1621 ggctacctga tccagcaggg aatgccggtg agttcggtta cgacaagggg cttcggaaag
     1681 actcagccag tggcttcaaa tgacaccgcc gcaggccgac aacagaatcg gcgggtggaa
     1741 ctggttatct ccggtgacat cattggtgcg gcgatcggct cgcctatcgc ggctaagtaa
     1801 gcccgtctgg gatttcaaac acgcagggcc ggcatccgat gcgcccccgg ccctgcgtga
     1861 aagcggcatg gaggaacctt tggtttaaca acggagttga gtcttgccac ccccggctcg
     1921 agattatagg aacgcgcatt ttgaggtctg aacgctatga atacgatagg aacagtaacc
     1981 aacgaaaaac aaggcctttc ttggcgcgac tggcaggaga ggaaccatct ccaggatctg
     2041 caccgtgtgg ccctgcgcgc gaagctgacg aagtatgctt ccatcgtggt gttgcttctg
     2101 gccgtggtgt tctggagcta cgcggcggac taccaggcgg tactggggtt cgtagtgtgc
     2161 gcgggcgccg tccgggtcgc attcctggca gccgccgtgc gcaggtacga ttgggcaagc
     2221 ctgttcattg gcatggccct gctttacaat ccggtcttcc ctctgtttgc actcactggc
     2281 cgcgtggctt tcttcctcgt gatcgccagc atcaccgcgt ttgctgcgtc gttgttcctg
     2341 ctgaagccgc gcctaacacc atcggtttcg gggtgctgaa cccatacacg ctggggcgat
     2401 ccgcggagtt cccggtctcc ggtggctggc atcagccgtt tactctccgc cccgccgggc
     2461 tcccgcacag ttgatctgtt cgggcatcgt gaaaacaacc aagttgccct tgttttgcat
     2521 ctgcctggct gccacttccg ccccggttgg cttctggctg agaaccttct gaaatgcctg
     2581 agttgttcga aagcggctta cgggagattt tcccgtacct gctgaagttg gaatgcgctt
     2641 gggacttcga gggcaagacc gcctggggcc tggagcagga aataaaagac tcaatccgcg
     2701 agcggcctga aaaggaactc accgacgatg aatcgccgga gcggtaggaa tacgggtgca
     2761 gcgattggtg cgggagaagg tgggcatcga ccggcggcag gttgccgttg ttcgaccatc
     2821 aaaggtgctg aatcactgaa gagcaacagt cagctgctca ggcggggaag atgcttctcg
     2881 tacaggagta ggaagtcttg cccctgctgt ccctctagcc agagaggttc ttgtacgccc
     2941 acaaaatgaa tctccgcccc ctcctgttgt ttgcctgcaa aacgtccctc cagttcctcg
     3001 gtacgcaaaa caatatagct gacataggcc gggttgattc ggtactcgcc gacggtgata
     3061 aatgatggtt ccattcgtgc aagtctagca tcgtcagaat cgaagactcc gtgctcgaag
     3121 cgtgaacgcg gcggtgacgc cgagttctga gctgaagcac gtgctccacg gtcccagttc
     3181 tcatgtcgaa caaacctgcg gcttgtagga cgtgattcca gcgtggtctg tcgccggcaa
     3241 agtgtcaggc cgatttttta cgccagtggt aaaccgaaag tgcagctaac gctccggcga
     3301 agagtgccac agtactcggt tccggaacct ccgtgagaaa atcccggatc tcactgccag
     3361 gagcacgctc tctccatttc cagcggcggg acaggaagga ggtcgcagat gagaatactt
     3421 ctggtcttga tcttctgcct cggtgcgcca gcagcgatcg cgcagtcagt tgtactgaaa
     3481 atcgcatatc agacctcctt gaacagttcg acctcgttgc cattaacttg cgccccctgg
     3541 cctggcagat gagcacaagt aacgttactc gatcggcaag aggtgttcag gagacgttct
     3601 tgtcctatgt gcccccaccc tcggattgcg gagttgggaa atgaagaggg tctctgaggt
     3661 tttgcagctc tcggtagtaa tagagggcag cggggtgccg tgctttgaac ttcagcctct
     3721 tcagaaactc gatctcctct tccgaggcat cgccgctgag ggcgctattc ttgagaaact
     3781 cctccagacc gggctccatc tcgaacggct gttcaggctc cctttcgaca aactcgaatc
     3841 tcttcagcgg gccaagcgcc agtctacggt tgaggacaat ctccacccca aacgtcatca
     3901 gatcgacgtc ccaggattcg atcagcggat cgaggaagga gacccagttt tcaaccgaga
     3961 cgtgaaagac atccgtttcc aggaactcga gaatcgtgac acgcatctgt tcataggtcc
     4021 ggccgctgag acgagccacc aggcggagcc agttttcact ctccagttct tctttggcaa
     4081 ctcgcttggc aacgtccaag agcttctgcg ttaccagccg ttcgagttcg ccgaagggct
     4141 ccttcccaaa gatcgcgcgg acctggtcct gcttggcagg cgcgcatttg cgaagcatca
     4201 actctcgaaa cccctgaaac aaagggcggg gaggttccgc cagcttcttc ttcaaggctt
     4261 ccctccgttg gaggtcggcc aacctcgcaa gctcaccgct cgggagcctc aagaactctc
     4321 ccatcttgtc gtagatgtcg gtccggttcg gtgccggcgg cgttttcttc cctcccagca
     4381 attgcgagat gtaggagtcg gtcacctgag cagcagcggc cagatctctc tgaccgaccc
     4441 ccaattcctt caaccggcgc cgaatcacca aggccaggtc cacaatgttt cctttgcgct
     4501 ttccattaac caaaaatatt taagtctaca gcgagagtat cgcatacttc cgcttgacaa
     4561 gaggaataat ttcacctaaa ctagttaatg actgagcaca tccgcccaat ctgcagatcg
     4621 tctgaactaa gcaggtcctc tgcagtttcc cctcttagcc aacccgaccg gggaggtcga
     4681 tatgttcgct cgcaccgtct ccatccgcct gaaaccaaac ggaattgtgg agttcagccg
     4741 ggtaatcgag aacgagacca ttcccctgct tcgaaaacaa aaagggttcc aggacgaaat
     4801 caccttcttc atcccaggcg gaagcgaagc cgtcgagatc agtctgtggg accggagaga
     4861 ggacgcggac gcctatggcc gcaaggccac cgcgaagttg cggaaggcct ttgagaacgt
     4921 gattgaggga gattccgagg ttcgcactta tgcggtttcg aattcgacgt tccacaagat
     4981 cgccgttccg gtctttgccc gaagctgagc gggttctcgc tgagagccaa gtccaccctc
     5041 agtgtcgtct ggcaaagagc aatgcacaca tagaggaaaa ggagatttcc aagtgcttac
     5101 gaagacaatg aagtggatca ctatcggggc gttagtcgca gcggcaatcc tgtggcgctc
     5161 cgccgcgaac tcgcaggttc cgcaattcct gctgggcttc gtcgtttgtt tgggcgcgag
     5221 cgtcgtagtg ctgcaagccg gccgggtgaa gaagttcgtc tgggctggtg gatttactgc
     5281 catcgctctg ctcttcaacc ccctcgtgcc ggtctttccg ttcggtggcg agtgggggcg
     5341 ctggctggtt ttgctcaccc tcgtgccgtt cgcggtctca ttgataacgc tgaggaccca
     5401 gccactgctg tcgatcccat cgatcacaga ccggaatccg ggaagtgaat cactgtagag
     5461 ctaagagtga gggttgggcg ggcgcgcaaa gggcgcccaa cctgaaggac ataccaaatg
     5521 acaagctttc gcatctttgc agtttcgctt tcgtgggttg tgctcgcttc gccgctgctg
     5581 tacgccggag atctctccag ataccgcagc tttcaactcg gagcggacct cccgacaatc
     5641 gccgggcagg cgcggatgga cccatctgag gcgaaggtgg tccatcgtct tccggcccta
     5701 atccaagtgc tcgagtggcg cccccagccc ctcgggccat cgacccgggt ggaatcggcg
     5761 gacaaggttg tcttcagctt ctacaacggc gaactgtacc gaatcgtggt caattacgac
     5821 cggtacaaca cggaaggatt gacggctgag gacctcatcg aggcgatttc agcaacgtat
     5881 ggaactgcga cgacgaatgc cgctgggatc accctgcagt ccgtctacga cgccaacgaa
     5941 aacgtgaaag tcatcgcgtg ctgggaggat tcgagctatt cgatgaacct ggtgcgttcc
     6001 tcataccagc caacttttgc attggtcgtc ttttcgaagc ggctggattc actggcgggg
     6061 gtggcggtcg ccgaagcggt ccggttggag aaggaacagg ctccccagag agagatcgaa
     6121 cgccaggaga aacaagcgga ggagaatcga gtgctgcaag agaaggccag gctggcgaac
     6181 aagccgggtt ttcgcccttg aacgccgcac gcgcgagcac cggcgccatg acagacacga
     6241 tcaaaacggg gacccttttg ttcgtcggcg cagcggaagg cgatggcctt tctctcggcg
     6301 cgattctccg ggaatccgac tggcagttgc acgggaccag gaattgccag gaggctctcg
     6361 agttcctcga caagaaccgt atctccgtgg ttctttctga agcagaactg cccgatggca
     6421 attggagaga actactgaac agcatggcaa aacgctctgc tgctccaagc ctcatcgtgt
     6481 cgtcccgttt ggctgacgac cgcctctggg cggaagtgct gagtctgggt ggcttcgacg
     6541 tgctgatgac accttttgaa acggaggagg ttttgcgggt gaccttcggt gcccggcaga
     6601 actcgcgatc gagagccgaa ggcttgcagc gcttaggcca acctggaggt cgaaatgagt
     6661 agcgtactgt ctatcaacgt gacgacgatc accatcatca tcgtggccat ttacctgttg
     6721 tcatccatca aaattctcaa ggagtacgag cggggcgtca tcttccgcct cggtcgcgtg
     6781 ctgcccaggg ccaaaggtcc cggaatcatc ttggtattcg cgccgattga tcgcatgacg
     6841 cggatctcat tgcgaattga agctctggag gtgccggcgc aggacgtggt cacgcgcgac
     6901 aacgtgaccg tgaaggtgaa tgcggtgatc ttcttccgcg tgattgatcc gcgtttggcc
     6961 gttctggagg tctccaattt cctgtatgcg acttcgcaac tggcgcagac taccctgcgc
     7021 agcgtcctag gggaagcgga actcgacgaa ttgctgagcc agcggggcaa attgaatatc
     7081 cggttgcagt cgatcttgga tcagcacacg tcgtcatggg gcatgaaggt gacgctggtc
     7141 gaggtgaagc aggtagagtt ggccgagcag atgatccgtg cgatcgcgcg acaggccgaa
     7201 gccgaacggg aacggcgcgc gaaggtgatc cacgccgagg gtgagtatat ggccgcggaa
     7261 aaactgggca tggcggctga agtcatccaa cgccagccgg cggcgatcca gttgcggtac
     7321 ctgcaaaccc tggtggagat cgggacggag aagaatacca ccgtcgtatt ccccctcccc
     7381 atcgacatga tttcgtcgct ggggcgtctt ctggacaagt tcgcaggtcc cggccgatca
     7441 acagtctccg aaagccgcgg cgacaccgtc ggctccatcg agtctcactg aggaggacac
     7501 cataatgttc aacgctcatc cagcaagaac gaccaagttc aaggagagtg acaacgtggt
     7561 attggccgaa ggcacatacc agggcgccac tggtgtattc atccggctaa ggcccgacgt
     7621 gaactgggcg gagatcaggg aacgcaacgg cacggtccgg tgccaccctg tcgtatggct
     7681 gcaacacgca gacccaccgg ggaagaatgt gtcaggctaa accggggaac cagaggattg
     7741 acgtgccggg tgaacaagcc gagtttttgc ccgtcgcgag cggaggcgcc atgacagaca
     7801 ccattaaaac gggaaccatt ctgatcgagg aaggcacacc cgtgccggat tctctactgc
     7861 tggaaagcga gccctattcg agtggttggg gatcactcag aaacctggat cttcgcggac
     7921 ttgatcgaga acttaccaaa accggctgga ctttcttttt catggccggt gagatcaggg
     7981 cgaccgcctt cggttttgac aggcgccggg cggtacgtga ggccgtgcag cgaatcatcg
     8041 caagtgtgaa gtcgcagaag tgtaattgtc tggaaatcag ccaagtggcg atgaagtctt
     8101 tcctgagagt gccctatgtg actgtctccg cccacgcgcg gcatatccag gagggtgtgg
     8161 tgtttctccg gccacggcag cgcgatcgaa ccgttcgact accggcggca gacccccgca
     8221 tccgacgcca gcggagcgaa atcaccgaag agcccttgtt tacggaagaa gcggttgccg
     8281 catgggaaga cgagggtgga gcaccggggc acatgggcgc ccgcctggcg gcgtaagcga
     8341 aggccgagct gagtgctgaa gacttggagt tcaaggagat tcttgtatga agattcgacc
     8401 gctctatgac cgcatcgtgg tcaaacgtat cgaagagcaa gagaccgtga agggtggcat
     8461 catcatcccg gacaccgcca aagagaagcc tcaggagggc gaagtggtgg cggtgggaaa
     8521 gggcaagcgc ctggaagacg gcaagatggt gcccctggac gttcaggttg gcgaccgcat
     8581 tctgttcggc aagtactctg gcagcgacat cgagttgggt ggcgacgagt acttgatcat
     8641 gcgagaggac gaggtccttg gaatcatcgg gaaaaccccg aagaagaccg gggcctagac
     8701 atcgggagag atttctatgg cggcaaaact gattatctac agcgaaaact caagacaagc
     8761 gattctgcgc ggcgtcaacc aactggccga cgtcgtaaag gtgaccctcg gtccgaaggg
     8821 ccgcaatgtc gttctggaca agaagttcgg cgggccggcg atcaccaagg acggcgtcac
     8881 ggtagccaag gaaatcgagc tgaaagatcc gctcgagaac atgggtgcgc agatggtgcg
     8941 agaggtggcg tcgaagacct cggacgtcgc cggtgacggc accaccaccg cgacgatcct
     9001 ggcgcagtcg atcttccgcc agggcgtgaa gagcgtcgcc gccggcgcca acccgatggc
     9061 tatgaagcgc ggcatcgagc gggccgttca ggcggttgtc gaacagatca agaaatacag
     9121 caagcccgtt tccggcgcga tgatcgccca ggtggggacc atctcggcca acggcgacac
     9181 gaccatcggc gacaggatcg ccgagtcaat gaagaaggtg ggcaaggacg gcgtcatcac
     9241 cgtcgaagag tccaagacca tggaaaccac aaccgacatg gtcgagggca tgcagttcga
     9301 ccgcggctat ctgtcgccct acttcgtgac cgacgccgag cgcatggagt gcgtgctcga
     9361 ggatccgtac attctgatcc acgagaagaa gatcagcaac atgaaggacc tgctgccgtt
     9421 gctcgaacag gtcgcccgtt ccggcaagcc gatgttggtg gtctctgaag atgtggaagg
     9481 cgaggcgctg gcgacactgg tagtcaacaa actgcgcggc accctgaacg cctgcgcggt
     9541 gaaagcgccc ggattcggcg accgccggaa agccatgctc gaggatatcg gaatcctcac
     9601 cggcggcaag acgattatgg aagagaccgg catcaagctc gagggcgtac ggctggatga
     9661 ccttggccgc gcgaagcgcg tcaccatcga taaggacaac acgaccatcg tcgagggcgc
     9721 gggcagccac aatgccatcg agggccgcat caagcagttg cgtgctcaga tcgaggagac
     9781 gacgtcggac tacgaccgcg agaagcttca ggagcggctg gcgaagcttg ccggcggcgt
     9841 cgcgctgatc aaggtcggtg cagccaccga gaccgagatg aaggagaaga aagctcgcgt
     9901 cgaagacgct ctgcacgcga ctcgtgccgc ggtcgaggaa ggaatcgttc ccggcggcgg
     9961 cgtggctctg tttcggagtg ccggcgcgct cgagatgcta aaagtcgaat ctgaagaaca
    10021 gatcggcgtc gatatcgtca agcgggcttg cgaggagccg ctgcgtcaga tctgccagaa
    10081 cgccggcttt gagggagccg tggtggccga aaaggttcgc gtcaacgaca acgtgaactt
    10141 cggcttcaac gccgagaccg aacaatacga ggacctggtg aagtcgggcg tgatcgaccc
    10201 gacgaaggtg acccgcaccg cgttgcagaa cgcggcctcg attgcctcgc tgatgctgac
    10261 gaccgaagcg atgatctgcg agattccgga gaagaaggag cccatgcccg gcggccacgg
    10321 ccccgacatg gactactaag ccgaaatctc cgattcggac tcctggcgtg tacaccagtc
    10381 tggaggtgga gtactctcaa cgtagccacg atcgccatca tcatcgtggt catccacttt
    10441 gttgtcgtcc atcaagatcg tcagagaatg cgagcgcgga gtaattctcc gcctgcacga
    10501 cccgaggcgg agcgagtttt tggatccgag gtcggctcat cctccatcga cgctacggcc
    10561 ctccgcgtgg ccgccgggct gtaggcgttc gtccaagcct actcgaccac cgcttgcgcc
    10621 ttttcacagg ttatcagctt gcagaacccg ttacggcggt catcgctgct atcctcaaaa
    10681 tccgctccct gtacaccggg cggcggttct gctgtcactc gctcccgcct caatacatca
    10741 ccctcgccgc tcgatctccc gcctgctcca gcgcgagccc tagatggtct gcgtgttttc
    10801 acgggcatcc tcagtcccgg cgatactaaa gagcttcgag gggacagaaa aggtaggtgt
    10861 acggtgagtg tccggcgaac tcatgtctgc cgaagtgagt gggtgcgatg cacggaggat
    10921 gggtgagtta ggagttgaag agcggcgaga ctggttcgtg ctgtcagcga ccaaaatatt
    10981 actacttagg tgcgatttta gttgacaata ttacgctcat attatataat ttagttgtac
    11041 ctaagaagaa agagaccaga gaccaaatgg aactagcaag gttcaacgct tacaatgatt
    11101 tccccacagg aatgcagctc ttccacgacg ccatcaaccg cctcctcaac gatgagaacc
    11161 aggtcaggcc ctggagccca gccgtcgaca tcttcgagac cgagactgag ctattcttca
    11221 aggtcgatgc ggcgggtatg agcctggagg acatcgatgt ccagctcgag aacggaacaa
    11281 tgacgctcaa gggcgagcgg aagttcgaga aggacgacaa ggtaaagggc taccatcgca
    11341 tcgaacgcag ctacggtgct ttcgcgcgtt ctttcacgct gcctaacacg gtcgatcccg
    11401 agggcgtgcg cgccgactat acccatggcg tgctgacgat cacgctgccc aagaaggagc
    11461 ttgccaagcc tcgggcgatc aaggtgaacg tcaactgagc caccgcggcg acgcggcaac
    11521 cccagagaat cttaatgttg tgacggcggg ctgctggaaa gcccgccgtt ggtttttgtg
    11581 aactgctcaa gaccgcgtac cgtccaaata ctcgatctgt ttgaaatcgc atgacttgca
    11641 gtgccccatc ctgcgcggac gtggcggacg tcacgcctgc tatcctggtg ccaatgcgcc
    11701 ggaccttcga cccgtttcga ctgttgttgg tttccatcgc cggttggttg agccaggagc
    11761 agcgtaacgc gatcgattac cttcacgaag agaacgaact gagtactgcg ggagcaactc
    11821 ggagagaaac gtcttcgact gaacgacgat caagaagaaa tctacgttcc gggccgtagc
    11881 cagaacgcgc accgtgacgc ctgacgccgc agctgcggtg gccgccagcc cggggcggta
    11941 aaaagcagcc ggaagagcgc ccccgggcgg tgacgcttgc ccttgggccg acgcggagtg
    12001 ggcgtacgct gctctggcgg ttctggagct ctccccgcga ccggaggcga ctccggagac
    12061 gagggcgagc ttgccggcgt cgaacagcga ctcgtcggaa aagtgtgccg tcgaaatgca
    12121 tggccagttt actgcgagac cacgatcagc gtggacctgc ccggcagggt gtagttcacc
    12181 ctcaggtacg actgcttgtt gccgtcgtcg tacgggttcg cagtaatttt gacacttccg
    12241 gtcggctgcc cttcgccgat cgtccatcgc aggcccatgt ggccggtctt ggagatattc
    12301 gagagattct gctggctgaa ggtgctgtaa acgttgcatc ccttgacgga tgaaagtgcg
    12361 gtgccggagt gggcgttcga tcccggaaac gttccggttg tggcgcgcga tccagagcgg
    12421 cggatctctt tctatgcaac gtcaatcgag gtcctagccg ggaggagggt tttggcgcgc
    12481 ttgtttccga gtagcgcgtg atccgaccgc gaacggcgga gcggttctac cgaagtccgg
    12541 aattgccgga gcccaggcac aacaggcagt cggcgacact ttgcaggtca tcgcaagggc
    12601 aagagcctgg gcggagcgct ttggatgagt ccccgagtac accctaagat agagagccaa
    12661 gtgagtagcc aaccgatgcc gacacacgcc ttccacaact ggccgtttcc gcgtctttcc
    12721 aagctgatca aagccggcaa catcggccac cgatcctacc gatgagtacc cgtcaatttc
    12781 tgcgcgcggg tgcccgcatc attcgttccg agaacatacg gacgaatctt tcgccatcca
    12841 cgacagccgg gtatgcggca tttgcgggca ctcgagggaa tccgacgatc acaaaccggt
    12901 cgccgcgcca ctctgagtgc tcggctgtga ggagttgatt ggtgacggtc gcgccgtagg
    12961 cgaattcgcc tttggtctcg atgtccacgt gaacgggctt cacggtcctg gccacctgcg
    13021 ggtcgatcgc aatgccaacc gcgagtgcgt cgtgaatggc cgcgccgccg ggaccgaatc
    13081 tctccgaaaa gctgaggtac ggctcagcga tggccccgac aaatctggct actggatcag
    13141 tgctccctcg caattgtgcc gcatcgttgc gggtgagacg tgcctgtgcg caggccgtca
    13201 gatccaccat cagcaagggg acgcccgact caaacacgat ctttgcagcc tcggcatcgt
    13261 ggtaaatgtt ggcttccgcc gccggcgtca cattccccgc tccgacagtt ccccccataa
    13321 ggatgatctc ccgtgttttc gccgccaagt ccggatactg caggaacgcc attgcgatat
    13381 tggtaagcgg tccgacaggc acaagcgtga tctgccctgg attggcttcg atcaaatcgt
    13441 ggattacctg tacggcgtgc cgggggtcaa gtccgatgtt cggcttcggt aagactaccc
    13501 cgcccagtcc gttttcgcca tgaatcgctt cggctgtgct cagtttccgc tgcaggggcc
    13561 gtgcggcgcc ttttgccacg atcacatccg tccgccgcgc cagttccacc agtttgcgag
    13621 cattttctgt tcccagttcc accgggacat ttccggcgac agtcgtaatt gcctcgacct
    13681 tcagttctgg cgaccgcaaa gccagcagga ccgccatcgc atcgtcgatt cccgggtcgg
    13741 tgtcaagaat gactcggcgg ggaaattccg gcactggact ctgacgtgtc gacgaaactc
    13801 cctgcgcgaa tgcgatgcat acggtgacca gcgctgcgca aatttgcatt gcaaccttat
    13861 agcacgcttt atctcgaata gcacggggcc gaggcagtag tcgccgaaga gtcgcctcac
    13921 cggaattcat ctattccggg tggtcgccaa accggaagtt gacctgatgg gcggcgccac
    13981 gcatctcaac ttccggtttg cgcgtcaacc ggccggagca ggcacaacgg taaacgctgg
    14041 gcgacttgtg gaccgaagag ggcgagagat catactgagc tccgccgacg cttagcgggc
    14101 cgaagacgga gcgggctgtg tctacctcaa gactgtcgcc tggcgcgttc acctcgcact
    14161 cgtcgcccgt gctcctgcct cggagcgtcg cctgtccgca gttaacgaat gacgaatttg
    14221 tgctcggcca tcgcgaccgt gggctggggt aggcccatcc gcgccaggca gttattgaac
    14281 cgcggatcgg cgcgcagaca atcccactgc ggcgcgacgg ccagatgtac caggcacggg
    14341 tcgcggccct ccagcgctcg ccctagatga tgaaacgccg cgtccagatc gccggcttcc
    14401 ccgtagagga ccgccagttg gatagccggg acgaggtccc cgccgctcgc catgcggcta
    14461 aggctgtacg tcaccaccgc ggcgcgcccg ccctccgcgt aagcccgctt cagttcgtcg
    14521 agcagctcgc cgggagcgcc gcagtcttcg gcgtgacgga tcgcctccgc catgtggcga
    14581 tcgtagtcgc cctttttcca gtaagcgccg gccaggtgct cgcgagcgag caggtggcgc
    14641 gggtccaact ccagcgcctt gttcgcccat tcgatcgatt cgtcataccg ccgctggttc
    14701 cagtacgaga gcgagatttg cagatgcact agcggcgaga acgggtcgcg ttcgagggcc
    14761 ttctgcttca actgaagacc ttcttccagt ttgccgatcg cttccagcag ccggccgcac
    14821 accaggtacg cttcggtgtg attcgggttc aactcgagcg cgcgccgcag gctcctctcc
    14881 gcgccaatcc agttccactc actgaggaag agcacgattc ccagcgcaac ctgtgcgtcg
    14941 gcgcaggagg catccaaagc cagcgccgcg agcgccgcag ctttcgcctg atggtaagcc
    15001 tccgcgtgcg gcatcacgcg gaactccgcc tgcgcgcacc acgcaagggc caaaccggcg
    15061 tgcgccgcgg cgtaggtagg gtccaacgca acggcagcct ggaacgccgc caccgctttt
    15121 ggcgtctcgg tcatggacgc cgccagcaaa tgcgctcgcc cgcgcccgat caactcgtac
    15181 acttctgacg tatggaccgg aagcagcggg cgcatcccga cgccactcac ccatcgatat
    15241 cctgagcgcg ccacagtttc gatgtactgt cgcctcccca gcgccttgcg caggtgcgaa
    15301 atatggaccg ccagtattcc ttcttcgaca aacgattccg gccacaccgc cgcaagcagt
    15361 tcgtccttcc gaaccagccg ccccccgttt cgaaccagca ctaccaacac gtcgtacgct
    15421 ttcggtgcta gcccgatcgc ctggccgtgc ttcgtcaatc tccgctcccc gacgtcgagc
    15481 gtgaattccc cgaactcata aacttcccgg ccagccattc gtttaatcgt atcccacgct
    15541 tcactgtgcc gcgtcgacac tcgcgtcgac gtccgggagc ttagatgaac gcttaatcga
    15601 aatttaagga atccttcaag actccctccc gcctttgcct ctactctgag cactatcagg
    15661 agggaaacat gccgaaccaa caagatcctt tcagcggaac gtggaaaatg aatccacaga
    15721 aatcgaactt cgaccccaat catcggcctt cggaggcaac catgcgcttc gagcgtgaat
    15781 ccgagggtta cctcatgcgg gcggagggcg tctgtgacgg taaacacgtc gaggagcagc
    15841 cccaaaggtt catcttggat ggcagggagc atcccgttcc cggcgtgctt gatgtaaccg
    15901 ccgtgtcgac tcgcccggac ccgaacacca tccgcgcagc ggcacgaagc ggcgatcgca
    15961 cggtcggcga gggcagctac gttgtatccg ccgacggagc aacactgaca gcaacggtac
    16021 gcggtatcga tgctcagcag cgcccgtttc aaactacggt ggtgtgggac cgtcagtaga
    16081 tccacgcctg ctaccctcgc ttcagatatg aatttcaaac ccgcgagcta gcgccaacta
    16141 tttcagttag gacgtcttcc acggcgtagt gtagaacaaa gttgctcaac tgccggatgg
    16201 tcggcaatcc gggagctatc cacatgagcg tatagtcgct agccttgatc ggaaatgtct
    16261 cggcagaagg aggcccgggc gaagcccgat gagctgggcc gagacgtttt cgataaggag
    16321 acttgaagga agccgctggc gtttgcggtg ggcgcggtca gctatgggat ttgaatgcca
    16381 ctttggtacc cgtagtaccc ggatcaggaa ttgcctcttt cagattggcg acgactcgca
    16441 tttgtgcttg tgatcctact ccagattggc tctcctccgc aagaggtagt gcgctaaaag
    16501 tgtggcggcc cgccctgccg aggccgccac cagggccaca cagatttagc gcactacccc
    16561 ttggggttct cccgcggctg gccagcaata cggctggcag cgggcgcggc ccttggtacc
    16621 tcgcccgggc caaggcgctg tccgttgggc tttcgaatgc gtacttcaaa tcgctcggac
    16681 ttccgacgct gatcgatggg tgttagcgta accgactcga accgccgtgt acggacccgt
    16741 acgcacggtg gtgtggcaga ggtcggtggg cgaccgccgc ccctatgccg atcaagtcgc
    16801 cgactcgtgg aagtgcctgc gaccgccgac tggccgataa tcagccgctg gtagtcgccc
    16861 agcgtttact gacgggcgcg ctaaacttcc ggacggatga cagcgtgtcc gggggttggg
    16921 cgacgcgatg ccgccttgtc gttcctaatc ctgccaactt ccggatagga ccaatcgtcc
    16981 cgaatctacc ggatcgggag cggccactct tgcagttcgc acgttatagc cacgcggcgg
    17041 gctcctctgt ggcgaggtgt gacgtcaggg tcgccgcatc attccaggaa caaccgcgag
    17101 cctgggatat cgaaagtcac tttgtagttc ttcatgaact ctccgccgag attcgcagag
    17161 tagtgctgct cagagccgag agttccggtg gtctgccgcg agaaacgtac gactggctta
    17221 tccgccgcga agcgtccgat gcggacttcc tcgatgcgcc cgaccatgtc aactgtggcg
    17281 cccccgaacc cgcgggtggt ccctggggcc acttcatcgg ctgagtcaag cagttgatgg
    17341 cggtcgacga agggcgagca tatggtcaga acgtgtgcgc tgccggtatc gagttgcatg
    17401 tcagcctcga tgggtctctg gccgcgcacg cgtatgctgc cacgcacgac gggcccaatg
    17461 gtcatgcgga tggggagcat gtcagccgtc ggtgatggct ggtatgaacc tggctggaag
    17521 atgcgcacgc gggagccggg gtagtcaatt tcgacaatgt acttgccgaa cagggggaag
    17581 cccaggattc cagccacagg gctctggaag gccagcgact tcatgtcgaa ggtgatggaa
    17641 cggggtggcc ggatttccaa ctcaccaagg ttcagatgca cgccaacaag caagccgacc
    17701 tgctgcgcgc cgccgcttcc ggaactgata gcgctgccgg actcgttcag accaagggca
    17761 gcggcacgcg cgcggtcgac aacgcaattc gcggcagcgg aatccaaaat gaacgtgagg
    17821 gtctgggctc catttactgt gacctgcacc aacgggagat tcttctccat cagcatgggt
    17881 agcacgacgg gccatcgcag ctccgcggcg gtcgccatcg ttgcctgggc gagtaaagca
    17941 atcgcgagta ggtgcatggt ctaaccgttg gacacctgcc tgcacgctga agttcccggc
    18001 gcatacgcat gtgtcgcgcg gcggcggcct gacgccaccg ctcgggatcg ctgatcgccg
    18061 ccagggcggc cgggcgccgg cgatcgcttt cccttgccgc accgcgcccg gacggaggcg
    18121 gcaccctgcg agccggaggt ggagactcca cggcgcgcag caccggtcgc aggcgttcaa
    18181 cgagcagcgc aggagggcgc gcttccgaca cacatcaaca attttttcga cccccgatct
    18241 ggtgtgggct tatgttcgcg ggaggagatt ggggaagact acgggcgctt tgtctagcta
    18301 tttagctaca ccggaagttc tccaccagca tcacgtcgga gccggtgtcg tcaatttgcg
    18361 tgtagagaat cgttcgacca tcgagcgaga cggctggcgg cctcgggaag tatgactcct
    18421 tcgcaagagc ctgcttggtg gcgatgtcaa agaactggat gaaccactta actccgggag
    18481 cggcaccttt tgctcgaccg cggaagtaga gacctttctc ggctatggcc cagcggccct
    18541 ccagtctctc gaggaccgag acctcctccc ctcccccgac gggaactttc caggcatcgg
    18601 ggacagcttc ctgggcaggg atctcccggt agagggaact cgaatggact gcaaatcgta
    18661 ttaaggaaat gcagacgcta tgaactgatt ctcaaacaaa gcaacccatc aatgaaggag
    18721 gcgccaaatg aaccgcgcgt acgcagtggt ggcatgcctc accctcgtca tcgccgcccc
    18781 tccccccacc tcagcccaaa cgaacctggg gacacgcgtc acgatcgacg ttaccgaggt
    18841 cgcgccgcag aaggtcttcg agttgctcgc ccgctcgctg gactgcacga ttacggtcga
    18901 cccggccgtg aagaaaccgt tgacgctacg ggtcgtggac atgccggcca ccgacattct
    18961 cagtatggtc tgccagagca tcaagtgcga gtaccggttc gacggcaaga acatctttgt
    19021 caaaccgctc tccgccagcc ggaggcgcca gatggcccta atggaagacc actccaggaa
    19081 actcatgagc cggctgccca cgggtatgca cttcgacggc acgccgttga aggacgtgct
    19141 cgacaccatc gcaaaggctg ccgggctcaa acttcggccc tggaaggacg aaggcggtcg
    19201 gaaggttacc atcgatgtag ggggcaagac cgtgaacgag gcgctcgagg cggtcgtgcg
    19261 ccagatcgac ggcgagggcg tcgtgatgat gcggacatgg gcgggttcct ggggccagta
    19321 ccgcttggtg gataagcgcc gcgagccgta gctgggcccg ggctggcagt tgtccgcgaa
    19381 agccacccct gactcctgat tcctggctcc cgaatcagac gatttgtttc tgagtccgac
    19441 cgacatgcgc aggacacgaa ggaggcgatc gtgacggtca acggatgaac aggggccggc
    19501 ccggtcgaac aatcggcttt tcacttcctc tttgccgacg actcactcgc aatccatggt
    19561 tgggagcgac gagaagatct acgctgcggt tgtcctgtct gatctgagga tgcggacaga
    19621 atgtagttgc tgggaccgcc tccggcggca acacaccccg ttcttcaatc cttgctaccg
    19681 gcaatacaat tggagtggag atcgtcgatg agcgcaacgg actccgaaca ctgcacttcg
    19741 gatgcgatac gccgaatcgg accccggatc tccggcctcc cggcctgggc at
//
LOCUS       DATIFQ010000092         3178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1753249, whole genome shotgun sequence.
ACCESSION   DATIFQ010000092 DATIFQ010000000
VERSION     DATIFQ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753249"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..684
                     /locus_tag="VLE22_04145"
     CDS             181..684
                     /locus_tag="VLE22_04145"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB13624.1"
                     /translation="MTSAKDLQALYDYCYWANEKLLDVVAQLTPEQFTQSVGGSYGSV
                     RNTLVHVLSAEWGWLDRCGGVKRGPALVATDYPTVTALVDRWRQVEAYVREFLSSLSD
                     EDLSRIVTFSLGSGQKYANRLGDLMHHAAIHGIHHRGQVSLLLRSLDQVPGNFDILLY
                     YGRNQTS"
     gene            717..1412
                     /locus_tag="VLE22_04150"
     CDS             717..1412
                     /locus_tag="VLE22_04150"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HSB13625.1"
                     /translation="MTRELTFRVSESAGEVSALLERPHQARWLLVLAHGAGAGMRHAF
                     MENLASELANAGIATLRYQFPYMQHRRGRPDPPAVLTATVRSAIGAAVETTPDLPLLA
                     GGKSLGGRMTSHVFSELPRAGATDAVARVIGLVFFGFPLHQPGRPDTKRADHLSRVTV
                     PMLFLQGTRDALADLSLVRPLCEGLAPRATLHIVDDADHSFHVLKRSGTTDADVLRDL
                     ARVTASWADSLRV"
     gene            complement(1426..2604)
                     /locus_tag="VLE22_04155"
     CDS             complement(1426..2604)
                     /locus_tag="VLE22_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HSB13626.1"
                     /translation="MGTEQDPNPRLGRYVVKMHNSETVRAYVPPPLPPNPPIRMEALQ
                     LQLEQANQSLGRLDGLASVLPNLPLFIYAYVRKEAVLSSQIEGTQSSLSDLLLFENDQ
                     APGVPLHDVQEVSNYVAAMNHGLDRLREGFPLSLRLIREIHGILLSKGRGSHKMPGEL
                     RRSQNWIGGTRPGNAVFVPPPPELVPDCMSALELFLHEKRPVLPALIKAGLVHVQFET
                     IHPFLDGNGRLGRLLITFLLCAAGVLREPILYLSLYFKQHRPVYYDLLDRVRTHGDWE
                     TWLDFFLTGVRDTAEQAAAAARRILALFEDHRRRIETLGRPAASVLRVFEHIQRNPIL
                     SIPSAAEKIGMSAPTVAKSLEHMRRLGILREITGRRRHRLFAYDTYLAILSEGTEPIR
                     "
     gene            2769..>3178
                     /locus_tag="VLE22_04160"
     CDS             2769..>3178
                     /locus_tag="VLE22_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HSB13627.1"
                     /translation="MFRKVLVANRGEIAVRVIRALRELRIASVAVYSDADRASLHVRM
                     ADEAVHIGPAPSAESYLRIDRILDAARTRGAEAIHPGYGFLSENADFAAACEDAGITF
                     IGPSSGAIRAMGSKTASRRLALEAGVAPVPGTTSP"
BASE COUNT          595 a          954 c         1026 g          603 t
ORIGIN      
        1 acgtccggcg caatctcatc gactctggcg gtcacaccgc acctcccatc cgggattcct
       61 atgttggact cccgagtctt aacgtagtgt ataccgatcc gtctctcttg taggcgaaaa
      121 gcggatgagt caaacgccgg cgccggatcg gtcattaccg ccggatgtac gatagttgag
      181 atgaccagcg cgaaggatct tcaggccctt tacgactact gctattgggc aaacgagaag
      241 ctcctcgacg tcgtagcaca acttacgccg gagcagttca ctcagtcggt aggggggagc
      301 tacggttctg tgcggaacac gctggttcac gttttgagcg ctgagtgggg atggctcgac
      361 cgttgtggtg gtgtgaaacg cggcccggcg ttagttgcaa ctgactatcc caccgtgact
      421 gcgctcgtcg accggtggcg gcaagtcgag gcttacgtac gagagttcct ctcaagtctg
      481 agtgacgaag acctcagcag gatcgtgacg ttctccttag gcagcgggca gaagtacgca
      541 aaccggcttg gtgacctgat gcaccacgcc gcgattcacg gaattcacca tcgcggccag
      601 gtttctctcc tccttcgctc gctcgaccaa gtacccggca acttcgacat cctgctctac
      661 tacgggcgga accaaacgag ttgaggctct caacgacgaa gctctaggga agtcttgtga
      721 ccagggaact cacatttcgc gtctccgaat cagcgggcga ggtatcagct ctgctcgagc
      781 ggccgcacca agcgcgctgg ctactcgtgc ttgcgcacgg cgccggtgct gggatgcgcc
      841 atgccttcat ggaaaatctg gcgagcgaac tcgcgaacgc tggtatagcc acgctgcgct
      901 accaattccc gtacatgcag catcgacgcg gaagaccgga cccgccggcg gtgcttacgg
      961 ccactgtacg ttccgcgatc ggtgcagcgg tagaaacaac accggatttg ccgttgcttg
     1021 cgggcggaaa gtcccttgga ggccgcatga cctcccacgt attctccgaa ctgccgcgcg
     1081 ctggtgcaac agacgccgtc gcgcgagtga tcggcctggt gttcttcggg tttccgctac
     1141 atcaaccagg ccggccggac acgaagcggg ctgaccacct aagtcgtgtg accgtgccga
     1201 tgctatttct gcaaggcacg cgcgatgcgc tggccgacct gtccctcgtg cgcccgttgt
     1261 gtgaggggct cgcgcctcgt gcgacgttgc acatcgtgga tgatgctgac cactctttcc
     1321 acgtcctgaa gcgctccggc acaacggacg ccgatgtcct gcgtgacctc gcaagggtta
     1381 ccgcttcttg ggcggactca ctgagagttt gatcgcatag caccactacc ggatcggctc
     1441 cgtcccctcg ctgaggattg cgaggtaggt gtcgtaggcg aacaggcggt ggcgccggcg
     1501 cccggtgatc tcgcgaagga tacccaggcg gcgcatgtgc tccagcgatt tcgcgacggt
     1561 gggcgcggac atgccgattt tctcagcggc ggatgggatc gacaggatcg ggttgcgctg
     1621 gatgtgttcg aagacgcgca gcaccgaggc tgcggggcgg ccaagagtct caatcctgcg
     1681 gcggtgatcc tcaaaaaggg ccagaatccg acgcgcggca gcagccgcct gctcggcggt
     1741 gtccctcacg ccggtgagaa agaaatccag ccaggtttcc cagtccccgt gggtccggac
     1801 ccggtccagc aggtcgtagt acaccggccg gtgctgcttg aagtagagac tcaggtagag
     1861 gatcggctcc ctgagaacgc ctgccgcaca gagcaggaag gtgatcagca ggcggccgag
     1921 ccgcccgttg ccgtcaagaa acgggtggat cgtctcaaac tggacatgga cgaggcctgc
     1981 cttaatgagc gccgggagaa ccggcctctt ctcgtggaga aataactcaa gcgcgctcat
     2041 gcagtcggga acgagctcgg gcggcggtgg aacaaagacc gcattgccgg ggcgggttcc
     2101 gccgatccag ttctggctgc ggcggagctc tcccggcatt ttgtggctgc cccggccctt
     2161 cgacaggaga atcccgtgga tctcgcggat cagccgcagt gacagcggga aaccctcgcg
     2221 cagccgatcg agaccgtggt tcatggcagc cacatagttg gatacttctt ggacgtcgtg
     2281 caacggcacg ccgggcgctt ggtcattttc gaagagcaag aggtctgaca atgacgactg
     2341 cgtcccttcg atctgagagg agagcaccgc ctccttgcgt acgtatgcgt aaatgaacag
     2401 tggcaggttg ggaaggaccg acgccagccc gtccagccgg ccaagagact ggtttgcctg
     2461 ctcgagctgg agctggagcg cctccatgcg gatgggagga ttggggggga gtggcggcgg
     2521 cacatacgcc cgaacagtct ccgaattgtg catcttcacg acgtagcgac ccaggcgcgg
     2581 attcggatcc tgctctgtac ccatgaagac ggaatctttt cgttagacgg ttccttacga
     2641 aaggatacac cgttttcgtt tcgtaagcca gtagagcctg tccgggctct ccccggagga
     2701 acgccgcccg gccgccgcca cacgaattcg aacggaaccc acgatcggac ttgctacaat
     2761 tgccctgcat gtttcggaag gtgctggtgg cgaaccgggg agagatcgcg gtgcgggtga
     2821 tccgcgcgct gcgggagctg cggatcgcca gcgtggccgt gtattccgac gccgaccgcg
     2881 cgtctctgca cgtgcgaatg gcggatgagg ccgtgcacat cgggccggcg ccgtcggccg
     2941 agagttactt gcgcattgac cggattctcg acgccgcgcg gacgcgtggc gcggaagcga
     3001 ttcatccggg ctacggattt ctgagcgaga acgccgactt tgccgcagcg tgcgaggacg
     3061 cggggattac cttcatcggg ccttcgtccg gcgccatccg tgcgatggga tcgaagaccg
     3121 cgtcgagacg cctggcgctg gaagcgggtg tcgcgccggt gccggggacc acgtctcc
//
LOCUS       DATIFQ010000093         2588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_604204, whole genome shotgun sequence.
ACCESSION   DATIFQ010000093 DATIFQ010000000
VERSION     DATIFQ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604204"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..2230)
                     /locus_tag="VLE22_04165"
     CDS             complement(302..2230)
                     /locus_tag="VLE22_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB13628.1"
                     /translation="MEGDALVGRRLCFGVFELDLRAGELRKRGLRVRLQQQPFQVLAM
                     LLEHAGEVVLREELQKALWPGDTFVDFDHGLNKAINKIREALGDSAESPRFVETVARR
                     GYRFIADVTVADVAPVRSPEPVPEPPPASDLRDRRDPAGSFAKITHRLTPRVWTASTF
                     VLIVLLASFAAWKLHFSNPPSSVIRSLAVLPLESLSSDASQDYFADGMTDQLITDLGQ
                     ISALRVISRTSVMAYKHTRKSLPQIARELNVDAVVEGTVLRSGDQVRITAQLIEGSAD
                     RHLWSQSYQGELRDTLALQNTVARAIADQIRINVNPQEQAALRTVAVVNPAAYTSYLK
                     GRYFWNKRTADGLRVALEYFNQAIDEDSKYARAYSGLADTYALLGDWQYAVMTPREAL
                     PKAKTAAIKALELDDSLGEAHNSLAFCLDGFDWDFDAAGKEFRRAIELNPGYATAHHW
                     YAWHLALLRRYDEAIVEMRKAANVDPLSLVINADLAELLALAHFYDESIRQSLKTLEM
                     DPNFGLAHNHLGQAYLQKHMNDKAVAELQEAVRLTGGSATCVANLARAYTASGKRSEA
                     ERLLSDLTKRSNPGQSYAPEIAAIYVALGDADHAMSWLEKGYEERFNPGVLIRPGFDP
                     LRSDPRFQDLVRRTGFRY"
BASE COUNT          518 a          772 c          798 g          500 t
ORIGIN      
        1 cccaatgctc ttgccatttc cgcggcattc atgatccgat attatactta ccgctaagca
       61 aaagcgaaag catggttccg ttcaaagctc cgccccgtac cgcgcaaccg ggggctgccg
      121 tgccgctgcg tgtcattctc aattggacac gcgccctcga gaagtgaggc ggcatctcgt
      181 tgtgcacggc ttcgatacgt ggagagtcgt cctgaccgag acatttgccg atgagatcga
      241 ctcgagcgac cagttggagt gcgtgcgctt gcacgtcttc cacacggaac gaagagcgtg
      301 ttcaataacg gaagccggta cggcgtacga ggtcctggaa tcgggggtcc gaacgaagag
      361 gatcgaagcc tggtcggatc aggacgccgg ggttaaaccg ctcttcgtag cctttttcga
      421 gccaactcat cgcgtgatcc gcatcaccga gcgcgacgta gatggcggca atttctggag
      481 catacgactg gccggggttt gaccgcttcg tcaagtcgct cagcagcctt tccgcttcgc
      541 tccgcttgcc ggaggccgtg taggcgcgag cgagattggc gacgcaggtc gcgctgccgc
      601 cagtcaacct gaccgcttcc tgcagttctg ccacagcctt atcgttcatg tgtttctgga
      661 ggtaggcctg gcccaggtga ttgtgggcga ggccgaaatt gggatccatt tcgagcgtct
      721 tgaggctttg ccgtatcgac tcgtcgtaga aatgcgcaag ggcgagcagc tctgccagat
      781 cggcgttaat gaccagagac agcggatcca cgttcgcggc ttttctcatc tccacgatcg
      841 cttcgtcgta gcggcgcaac agagccagat gccacgcata ccaatggtgc gcggtggcgt
      901 aacccgggtt cagttcgata gcccggcgga attccttccc ggcagcgtca aagtcccagt
      961 cgaagccatc caggcagaac gcgagcgagt tgtgggcttc gccaaggctg tcgtccagtt
     1021 ccagggcctt gatcgcagcg gtcttggctt tcgggagagc ctccctgggg gtcatcaccg
     1081 cgtactgcca gtcgcccaac aacgcgtagg tgtcggccaa gccagagtac gctcgtgcgt
     1141 atttcgagtc ctcgtcgatc gcctggttga aatattccag cgccactctc aaaccatccg
     1201 ccgttcgctt gttccagaag tagcgtccct tgagataaga cgtgtacgcg gcaggattca
     1261 caactgcgac ggttctgagc gccgcttgct cctgagggtt cacgttgatc cgaatctgat
     1321 cggcaatggc tctcgccacg gtgttctgga gcgccagcgt gtcccgcaac tcaccctggt
     1381 agctctggga ccacaggtgc ctgtctgccg aaccctcgat caactgcgcc gtgattcgga
     1441 cctggtcgcc ggaacgcagg acagtgcctt cgaccacggc gtccacgttc agttcacgag
     1501 cgatctgcgg cagagacttc cgcgtgtgtt tgtaagccat caccgaggtg cgcgagatca
     1561 cgcgcaacgc gctaatctgg ccgaggtccg taatcaactg gtcagtcatc ccatcggcaa
     1621 agtagtcctg ggacgcatcg ctcgagaggc tctccaatgg cagcacagcc agggaccgaa
     1681 tgaccgatga cggagggttg ctgaaatgaa gcttccaggc ggcgaaagag gccagcagga
     1741 cgatcagcac gaacgtcgat gccgtccata ctcgcggcgt cagccgatgt gtgatcttcg
     1801 cgaaactccc ggcgggatcc cggcgatcac gaaggtctga cgcgggagga ggctcaggta
     1861 cgggttccgg gctgcgaaca ggggccacat cggcgaccgt aacgtcagcg atgaagcggt
     1921 agccgcggcg cgcaacagtc tcgacgaacc gcggactctc ggcggagtcc ccgagggcct
     1981 cacgaatctt gttgatcgct ttgttgagcc cgtggtcgaa atccacgaac gtgtcgccgg
     2041 gccagagtgc cttctgaagc tcctcccgaa ggacgacctc tccggcgtgc tccagcagca
     2101 tggccaggac ttgaaagggc tgttgctgaa gccggactcg caggccgcgc ttgcgcaatt
     2161 cgccggcacg aagatccagc tcgaataccc caaaacagag gcgccggcca accagcgcgt
     2221 ccccttccat atgacgaggt tgaagggtac cacacgtctg cccgaccgct ggaagggact
     2281 gggcccggta agcctcttgc ccagtgatga ttacgagggg acccacgttc cagcagagag
     2341 gacttcccgt ccattgtgta gatcccgagc gcaaaccata ttgacagctc gcaccgcgca
     2401 ggctggcggc atgcggcgaa cgggacttga agaacgaagt agcagacgag ggaggacgag
     2461 gatgtctgag atgaaaggtg acgagctcct gcacgatcac agcaacgacg gattcgaccg
     2521 gcgggggttt ctgaagtgca tggcctgggc cggaaccggc gcgttccttg tcatgcaggg
     2581 tggcgtcc
//
LOCUS       DATIFQ010000094        21996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2328028, whole genome shotgun sequence.
ACCESSION   DATIFQ010000094 DATIFQ010000000
VERSION     DATIFQ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 21996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21996
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328028"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VLE22_04170"
     CDS             complement(<1..511)
                     /locus_tag="VLE22_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB13629.1"
                     /translation="MIAVAALGLCLLNAQTRVPAESYRKQALEFSKKKLWSQAIANYR
                     KALALEPADPDTHYNLALALKYSGNPGAAVGEFEAALRLKPDWPSAHFGLGAAEYERH
                     NQAAALREFRAALAGDPNNAGTHYFLAVVLMDRREFVEAIAELKKTLELRPSHSEACV
                     ALGLCYLRAQ"
     gene            complement(551..4033)
                     /locus_tag="VLE22_04175"
     CDS             complement(551..4033)
                     /locus_tag="VLE22_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB13630.1"
                     /translation="MRVRIGVALAVLSVVIAPALLRADVTGTILGLVTDSTSAVVPGV
                     RVVAINLGTSQRQEATSDSAGQFRLLALPVGRYRVEATAQGFQKFVTTAFDLSVNEQR
                     RVDVVLQVGNIEQQIEVSANALQIETTNTQLGEVVEEKKVLSLPLNGRSYIDLLGLQA
                     GVAPTTAGSMQQDRPVSGGLSAGNVSVNGQRETANAFLVNGGDVSEGRNLGASVIPNL
                     DSVAEFRLITNSFDAEYGRFSGAVMNAVTKSGTNGFHGTMFEFLRNDKLDARNFFDPE
                     KGALKRNQFGYSMGGPILKNKLFWFTDYQGTREVRGIGTGLVVLPTAAMRGGDLAAAP
                     GFVASSPDEAPTVNGSYWAGVLSQRLGYQVTAGMPYSFAGCTSPADCVFPGGVIPSRA
                     FSAPVPATMKYIPLPNAGEDTFASAGQNQKLVDDKAGQRVDFLNKLTGNWSFFYFFDD
                     ATLTSPLPASSVPGFPSVTPTRAQQGVLSNTRVFGPTAVNEARLSFMRTATITDKPQA
                     GFAKVGDLGFITGIGTLGIIPSGPPGFEAVPPISFNDFSIGSPTLTTKQPNNTWHASD
                     IFSKVWSKHTLKLGGEFRYLQINERNVCAPNGSFSFDGSETGSDWADYLLGAPSEYIQ
                     CSMQFLDSRTKYGGAFAQDSWRVKPNLTLNLGLRWEFSMPWYDTQDKIETIVPGLQSV
                     VFPTAPKGWVVPGDPGIPSTLSPTDYNNLGPRFGLAYSPGFSDGVLGKVFGGPGKTSI
                     RLAYGIYYTAVEDLTLFYEVGDAPYGLYWPSIVPPLFEEPFRTRSDGSSQTQRFPFKF
                     PIPGDPALKTLDYSIYLPIQSSPGYKTDNVQPYAMHFNFTIERTLSPSMILRLGYVGT
                     QGRKLIAQYESNPGDPALCLSLRGSGVMEGTPECGRNGELGVYTRPDGTIVNGTRGPL
                     GPNFGSNGYTATAANSSYNSLQVTLERKAADLTFLTAYTFGKAMDNSSGFNEWINFTN
                     FGLSRSLSQYDITHNFVVSYNYAVPFDRWLGQNRFTSGWSLNGITRFATGFPVNIRQS
                     GDYSLVGSGSTDVPDLVGSVRTQDPRNSGPNGEANLYFFTDAFSLAPLGRFGNSNRRF
                     FHGPGFNNWDFGVHKDTRIAESMAIQFRLEFFNLFNHAQFYNPAGNIRSAQFGQVSGA
                     RDPRIGQMSLKFLW"
     gene            complement(4209..5690)
                     /locus_tag="VLE22_04180"
     CDS             complement(4209..5690)
                     /locus_tag="VLE22_04180"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSB13631.1"
                     /translation="MSTPVHTLPNFIGGEWRASSAAETLGFLNPATAETLGRVPISTA
                     SEVDEAARAAAAASIEWRRVPVMERIQPLFKLKGLLETHFEDLSSTITLECGKTLAES
                     RGEMQRAIENVETACGIPILMQGYLSEDIARGIDEYMIRQPLGVAAVIAPFNFPGMIP
                     FWFLPYAIACGNAFLVKPSEKVPLTMHNVFRLIEQIGLPKGVVSLVNGAKETVDAILD
                     HPAVRAVSFVGSTPVAKYVYARAGANGKRAQCHGGAKNPIVVLPDADMEMTAQISADS
                     AFGCAGQRCLAASFAVTVGEARAPFTDAIVSQAEKRKVGNGLDEGVQMGPVITSESKR
                     RIETIVQKGLDAGARAVVDGRGPKISGHEDGFFVRPTVLDNIAPGTEISRTEIFGPVL
                     SLMHVETVDEAIGLVNSGTYGNMACLFTSSGAAARKFRNEVEAGNIGINIGVAAPVAA
                     FPFSGARESFFGDLHGQGRDAVEFFTQEKVVVERWPREWSRKF"
     gene            5853..7955
                     /locus_tag="VLE22_04185"
     CDS             5853..7955
                     /locus_tag="VLE22_04185"
                     /inference="COORDINATES: protein motif:HMM:NF044450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13632.1"
                     /translation="MPFPKGFLKDVGRLRLYDAGGKPVAASFRVVNRWWDDASTQVAS
                     IQWAHFDFLADAPANGKAVYRLRLSDEEAPAPSAPLRVETDGDRIRVNTGAIAFSVSR
                     SGPFLEAPGLKDADILLRSDERIYKASNGPATELVVEEQNPLRVVLKRTGSHGWVDGE
                     DRALDYVMRIVAWAGRPQIRIVYSFVNRQGQQMSDFVRLDGLWLQYRLERAAEPQGIE
                     QLSAEPRRQGWFEAGGIGVGLRWFWQLYPKGFEVRQDGTVRLALFPETARPQNIYKGV
                     AKTHEMILSFGGENLSAHLDHPLYAVAPPKWYTRETKALGRLVESSPEAYRPEHWPLV
                     ERYDRWLTACRDAVLQKRDRGFQFQDRRYDEYGMLNFGDAMHKLVTNDRRPDFGIHWE
                     TEYYDFPHALFLHFFRTGDMKSFQTAVEAAAHLADVDISHDETVPKRNGAARTGPGLN
                     HWTRYSNGVFISSSSWAFYKNESLFDRYLLTGDLWSRDVARLSSSFGTEYNGLDLQSN
                     TRSIGHGLFAMLKAYEVFGDKKYLDRAHWIVDCVQAWQDGDVAKLKVLNQRAVWDPQF
                     KGGYSHQSWMYGIALEGMAQASMTLGRPEMPGYLRRAADWIFANPKEWDPNRRRFLNS
                     DTLAPMLTPGLAYIAETSGDRKYWDIALENFTRHVEEGRTTDRLKLFAQLFRNSQRFL
                     WYLSAEAGKEAAGASGSE"
     gene            8023..9168
                     /locus_tag="VLE22_04190"
     CDS             8023..9168
                     /locus_tag="VLE22_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSB13633.1"
                     /translation="MEALRPETPDQLAESLITAASAGRTITLGGAFSKNGMAGPVKPS
                     DVTISTTGLNRILQYEPRDLTISVEAGIRYEDLARALEENGQILPLDPPFSSEATIGG
                     ILAANTCGSLRRLYGTARDLLIGMKFATLEGKLVQSGGMVVKNVAGLDMGKLLIGSFG
                     TLAAIAVANFKLVPKPPCSGTFLLEFEDVAQAMETRDRILHSVLQPTALDLLNAAAAE
                     QLGREGCLIALQAGGNAAVMERYRRELGAARAQDEGEEANFWTGVREFTPHFLKRNPE
                     GVIVRASCTLAQVREVMESFTGPAVARAASGVCYGYFSENGAASEWMERATVNGWRPV
                     IEFAPESKKTGLDLWPGPGSDFDTMKKVKGMFDPGNLLNRGRLYGRL"
     gene            9155..10459
                     /locus_tag="VLE22_04195"
     CDS             9155..10459
                     /locus_tag="VLE22_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSB13634.1"
                     /translation="MAVSEEVRRDAPRQFDLDKCVHCGLCLNSCPTYRELHLEMDSPR
                     GRVYQMVQVAGGAMAIGPSYIEHIGLCLACRACETACPSGVPYGRLVEAARAEIRARM
                     PRSPAVEVLRRFVFEHLLQSPALLQIAGALLYAYERTGLQRLVRSSGLLKLFGELGGI
                     EQLTPHAEWPFFFRNIGQTFPAEGTARYRVAFHAGCIANVCFARLNEATVRVLRKNGC
                     EVVIPAEQNCCGALHVHSGEREEARKLARRNIDAFLAGGYDAIITNAAGCGSTLKEYH
                     ELLEDDPDYRERAQRFVKLVKDVNEFLASIELNREMGSVPVTATYQDSCHLAHGQRIR
                     IPPRKLLEAAPGLRFVEMPLSDICCGSAGIYNVVQHEMAMAILRKKIQTVNGTGADVV
                     LTANPGCMLQLRAGVELYGKGQRVAHVVEILDEAYRNYSAGK"
     gene            complement(10583..10684)
                     /locus_tag="VLE22_04200"
     CDS             complement(10583..10684)
                     /locus_tag="VLE22_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HSB13635.1"
                     /translation="MDNTTLSAVLWIAAGAMLALYLLRRRKRKMGGR"
     gene            complement(11094..12461)
                     /locus_tag="VLE22_04205"
     CDS             complement(11094..12461)
                     /locus_tag="VLE22_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HSB13636.1"
                     /translation="MAAAPITQVESRAHILGVGAAASKSPVSPHDLPANLIEMKIAIH
                     TRLLDTLNLDQLGNMVNERARTEVRASIGRAIDDLRLPLNGERETVIDQVLEEVYGLG
                     PIEPLLEDPTISDILVTTPKMVHIERAGRLYETAVQFRDDAHLVRVMEKIVSRVGRRL
                     DQTSPLVDARLADGSRVNAVVPPVAVDGPLLSIRRFGHSPLPAEALVKNLSLTEGMLE
                     LLRACVKARLNIIVSGGTGAGKTTLLNILSGHIPEHERIVTIEDAAELQLRQRHVARM
                     EARPANIDGQGAIRIRQLVINALRMRPDRIIVGEVRGEEALDMLQAMNTGHDGSLTTV
                     HANSPRDAISRLETMVGMANANIPIRAIRQQMSSAINLFVQIARMTDGSRRVTHITEC
                     CGMEGEQVTIQDLFVFEKTGLADDGRVKGRFRATGIRPKFSERLAAAGVQLPASLFQV
                     VVELR"
     gene            complement(12710..16930)
                     /gene="rpoC"
                     /locus_tag="VLE22_04210"
     CDS             complement(12710..16930)
                     /gene="rpoC"
                     /locus_tag="VLE22_04210"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSB13637.1"
                     /translation="MYRSSPYDRANLIADFDSIRISLASPEKIRSWSHGEVTKPETIN
                     YRTFKPERDGLFCARIFGPTQDWECLCGKYKRMKHRGVICDKCGVEVTLARVRRERLG
                     HIELASPCSHVWFFKGLPSRIGYLLDITLRELERVLYFEAFVVVDPGELTTLSTGEVI
                     SDERKRQLDQEFPGKFVAMMGAEGIKELLKKIDVESLSLDIREKMKTEVSQQKKLKFA
                     KRLRVVESFRKSGNKPEWMILDVLPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKKLIELHAPDVIVRNEKRMLQEAVDALFDNGRRGRVLRGANNRPLKSLSDTLKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPDLKLHQCGLPKKMALELFKPFIYHRLEQRGHCT
                     TIKQAKELVEQQDPVVWDILEEVIRDHPILLNRAPTLHRLGIQAFEPVLVEGKAIKIH
                     PLVCTAFNADFDGDQMAVHIPLSPEAQIEAATLMLASNNILSPAHGSPIAVPTQDMVL
                     GLYYLTKARPKTKGEGRTFASNDDVLIALEMGEVETLTPIKLRYSGKVIDLTKAFDNQ
                     NVLHTEPIEFNKQYMDTTVGRVILNDVLPDEMPFINGLLKKKGLTQLVQHCYLRFGLP
                     TTVQMLDRVKDLGFLYATRAGISIGIDDMVVPGEKAKLVKDAEKQVMQVQQQYQEGAI
                     THGERYNKIIEIWSKVTERVSDEMFKVMEEDDRTGRYLNPIYIMADSGARGSKQQIRQ
                     LAGMRGLMAKPSGEIMETPITANFREGLNVLQYFISTHGARKGLADTALKTADSGYLT
                     RRLVDVAQDVIVSEFDCGTNDGIFVEPIVESGEIIEPLRDRIVGRVALEDVLDYDQNI
                     LIRANQEISEELAALIQASGIERVKIRSVLTCESKRGACQLCYGRNLATGRLVERGEA
                     VGVIAAQSIGEPGTQLTMRTFHIGGAATRVSEQSTQDAKSGGKVKYLGIVTVRNHLGE
                     LIAMNRNGILAVVDEKGREKERYQVVYGAKILFEDGASVEANQVLLEWDPYTFSILTE
                     VSGTVHFRDLVDGETLQEQVDEITGMSQWVVVDAPDAKRMPTVMIRPAGGTRSEEKRY
                     LMPTHAHLMVRDGEEAHAGDVLAKIPRATTKTKDITGGLPRVVELFEARKPRETAVIS
                     EINGAVKFGEVSKGFRKVYVVGDDGQQREYLIPRGVHINVQEGERVRAGDPLMDGPRN
                     PHDILHVLGEKELQKYLVNEIQEVYRLQGVNINDKHLEVIVRQMMRWVKIEEIGDTEF
                     LPEEVVDKFKFREENLRVVEAGGRPADGKAVLLGITKASLSTDSFISAASFQETTRVL
                     TEAAINGKVDHLRGLKENVIMGRLIPAGTGMEYYRRVKIAGEDIEEEVMTEEPEGPLE
                     GIPGYEDETRLHYTGGLSEDIGDQSLAE"
     gene            complement(16994..21298)
                     /gene="rpoB"
                     /locus_tag="VLE22_04215"
     CDS             complement(16994..21298)
                     /gene="rpoB"
                     /locus_tag="VLE22_04215"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSB13638.1"
                     /translation="MQNPASSANRERIDFSNIKTSIPIPNLIEVQKKSYERFLQMDLL
                     PDEREDVGLQSVFNSVFPITDFRGLSQLEFVDYSIGNWECKCGNLKGLNHLRTTCRNP
                     GCGAYIRTDPFHAGDVLCPKCGTFNKNIVTFCNRCGDPVGLQLKYEVPECEERGMAYA
                     APLKVTIRLTVYDKEGETGQKTVRDIKEQEVFFGEIPLMTDNGTFIINGTERVIVSQL
                     HRSPGVFFEKIPAQGYFLGKIIPYRGSWVEFEYDAKNLLHVRIDRKRKFYGTVFLRAL
                     GLKSDEQILRTFYKVDKITIRENRLFWTVSPGLVGLKLSHAVTTRGGDTVVAAGKKIT
                     TAVLKELQKAKIEHVEVAPNDLEGAYIVADVVDMSTGEVLMEANQELTTASVSKLIEG
                     GTPSFEVFFPERDEVGNVISMTLRKDSVRSQNDALLEIYRKLRPGDPPTLDTAQQLFS
                     GMFFDPRKYDFSRVGRMKFNIKLYGKADATPLDQRTLSAEDFNETIRYVLRLRRGIGS
                     VDDIDHLGNRRVRAVGELLENQFRIGLVRMERAIKEKMSVYQEMSTAMPHDLVNAKPV
                     MAAIREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHPTHYG
                     RICPIETPEGPNIGLISSLSCFARINDYGFIESPYRRVLDSQVIDEVKILNPGDTNHR
                     VGDIVFRNDVERANRALSAKKQPAEYEAYCTYLSAWEEDKYTIAQANVELDSESRLTA
                     ELVNARQAGNFLLMERDAVEYMDVSPKQLVSVAASLIPFLENDDANRALMGSNMQRQA
                     VPLLRAEAPYVGTGMERVTARDSGAVRICKRAGVVDSVDSERIIVRVEGGAHEGQMSR
                     EVGADIYQLTKFKRSNQNTCISQKPIVSHGQRVKKGDVLADGPCTDNGELALGRNVLV
                     AFMPWRGYNFEDAVIVSEKLVKEDYYTSIHIEEFEIEARDTKLGPEEITRDIPNIAES
                     FLRNLDESGVIRIGATVKPGDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVKDASL
                     YCPPGIEGTVVDCKIFSRKGAELDDRSKQILDDEIARLHRNLEDEKRILNDERAKRLA
                     GLLEGKLLLADLHDEKTNKRLLVKGMALERDVIEKLRSRDLKRMKLKDKDPRLNDEID
                     QIEEMTSRQVAVLEKIAEEKTAKLKKGDELPPGVIKLVKVYIAMKRKLSVGDKMAGRH
                     GNKGVIARIVPDEDMPHLPDGTPVEIILNPLGVPSRMNVGQILETHLGWAAHALNRVY
                     ATPVFDGATERDIKKELVEANLPTSGKITLRDGMSGLPFEQPVTVGYIYMLKLSHLVD
                     DKIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYILQELLTAKSDDV
                     YGRAKIYEAIVKGEAAAEPGVPESFNVLIRELQSLCLDVELMKKPRELLETALAAD"
     gene            complement(21750..>21996)
                     /gene="rplL"
                     /locus_tag="VLE22_04220"
     CDS             complement(21750..>21996)
                     /gene="rplL"
                     /locus_tag="VLE22_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HSB13639.1"
                     /translation="GGGAAAPAAEEKTEFTVVLTAAGANKINVIKAVREVTSLGLKEA
                     KDLVDGAPKPVKEGISKEEAETIKKKFVEAGATVEVK"
BASE COUNT         4105 a         6412 c         7059 g         4420 t
ORIGIN      
        1 tctgggcccg taggtagcac aagcccaagg ccacacatgc ctccgagtgc gaggggcgaa
       61 gttcgagggt cttcttcagc tcggcaatcg cttcgacaaa ctccctccgg tccatcagga
      121 cgacagcgag gaagtagtgg gtgccggcgt tgttcggatc gccggcaaga gccgcgcgga
      181 attcacgcag cgcggcggcc tggttgtgcc gctcatactc ggccgcgccg aggccgaagt
      241 gtgcggacgg ccagtccggt ttcaagcgca gggcagcctc gaactctccg acagccgcgc
      301 cggggtttcc gctgtacttg agggcaagag cgagattgta gtgggtatcg gggtccgcag
      361 gttcaagcgc gagagctttc cggtagttgg caatcgcctg gctccagagc ttctttttcg
      421 agaactcgag agcctgcttg cggtaagact ccgccggtac gcgggtctgt gcattcagca
      481 aacacagacc cagcgcggca accgcaatca ttcccatcct ggcttgcagt gtagcgaatt
      541 ccgaaaccgc tcaccaaagg aacttcaagc tcatctgtcc gattcgagga tcacgcgcgc
      601 cggacacctg tccaaactgg gcgcttcgga tgttccccgc cggattgtaa aactgcgcat
      661 ggttgaagag gttgaagaac tccagccgaa attgaattgc catgctctcg gcaatcctgg
      721 tgtctttgtg aaccccaaag tcccaattgt tgaagcccgg tccgtggaag aaccgccggt
      781 tggagtttcc gaaacggccc agcggcgcaa ggctgaaggc gtcggtgaag aagtacagat
      841 tggcctctcc attggggccg gagttccggg gatcctgggt cctgacagag ccgacgagat
      901 ccgggacgtc ggtgcttccg cttcccacga gcgaatagtc gccgctttgc cggatgttca
      961 ccgggaatcc ggttgcaaaa cgggtaatcc cgttcaagga ccaaccggag gtaaacctgt
     1021 tttgcccaag ccagcggtcg aagggcaccg cgtagttgta actgacgacg aaattgtggg
     1081 tgatatcgta ctgcgagagc gagcggctca ggccgaagtt ggtgaagttg atccattcgt
     1141 tgaacccgga cgaattgtcc atcgccttgc cgaaggtgta ggcggtgagg aaggtgaggt
     1201 cggcggcctt acgttccagg gtgacctgga gggagttgta actggaattc gccgcggtcg
     1261 cggtgtagcc gttgctgccg aaattcggcc cgaggggccc ccttgtgcca ttgacgatcg
     1321 tgccgtccgg tctcgtgtag acgcccagtt ccccgttgcg gccgcactcg ggggtccctt
     1381 ccattactcc gcttccgcga aggctcaggc acagggccgg atcgccggga ttggattcgt
     1441 actgcgcgat cagcttgcgg ccctgagtac ctacgtagcc aaggcggaga atcatggagg
     1501 gactcaacgt tcgctcaatc gtgaagttga aatgcatggc gtacggctgg acgttgtcgg
     1561 tcttatatcc aggtgaactt tggatgggga ggtagatcga gtagtccaga gttttcaggg
     1621 cggggtcgcc cggtatcggg aacttgaagg ggaagcgttg ggtctgggaa cttccgtcgg
     1681 aacgcgtgcg gaagggctct tcgaacaacg gggggacgat gctcggccaa tagaggccgt
     1741 agggggcatc gcctacctca tagaacaacg tcagatcttc gacggccgtg tagtagatac
     1801 cataggcaag gcgaatgctt gtctttccag ggccgccgaa gacctttccc agaacgccgt
     1861 cggagaatcc cggcgagtac gcgaggccga aacgcggacc gaggttgttg tagtccgtgg
     1921 gcgaaagcgt gctcgggata ccagggtcac ccggtaccac ccaccccttg ggggcggtcg
     1981 ggaacacaac cgattgcagg cccggaacga tggtctcgat cttgtcctgc gtgtcatacc
     2041 agggcatgct gaattcccag cgtaggccaa ggttcagcgt gaggttcggt ttgacgcgcc
     2101 aggagtcctg ggcaaaggcg ccgccgtact tggtgcgcga gtccaggaac tgcatgctgc
     2161 attgaatgta ttcgctgggc gcgcccagga ggtagtccgc ccagtcgctg ccggtttcag
     2221 agccgtcaaa gctgaagctc ccattcggtg cgcacacgtt ccgctcgttg atctggagat
     2281 accggaattc gccaccgagc ttcaaggtgt gtttactcca gactttcgag aaaatgtccg
     2341 aggcatgcca ggtgttattc ggctgcttcg ttgtaagggt ggggctaccg atggagaagt
     2401 cgttgaagga aatcggggga actgcctcga aacccggcgg tccggaggga atgattccaa
     2461 gcgtcccaat cccggtgatg aagccgagat ctccgacctt agcaaagccc gcctgcggtt
     2521 tgtccgtaat cgtcgccgtt cgcatgaagc tgagacgggc ttcgttgaca gccgtcggac
     2581 cgaaaacgcg cgtgttactc aggacgccct gctgggcgcg ggtcggcgtc acgctcggga
     2641 aaccgggcac gctggaggcc ggcaggggac tggtaagcgt ggcgtcatcg aagaagtaga
     2701 agaaggacca gttcccggtg agcttgttca gaaaatcgac gcgctgccct gccttgtcgt
     2761 ccacaagctt ctggttctgg ccggcactgg cgaaggtgtc ctcgccggcg ttcgggagtg
     2821 ggatgtactt catggtcgcg ggcacgggag cggagaacgc ccgcgacgga atgacccctc
     2881 caggaaagac gcaatccgcg gggctcgtgc aaccggcgaa gctgtacggc attcccgcag
     2941 tcacctggta ccccaagcgc tgggaaagca cgccggccca ataggagccg ttcaccgtgg
     3001 gggcctcgtc cggggatgag gccacaaatc ccggcgcggc ggccaagtct ccgccccgca
     3061 tagcagccgt cgggagcacg accaaaccgg tcccgatgcc acggacctcg cgtgtgccct
     3121 ggtagtcggt aaaccagaac agcttgttct tgagaatcgg tccgcccatc gagtagccga
     3181 actggttgcg cttcagcgct cctttctccg gatcgaagaa gttgcgggca tcgagtttgt
     3241 cgttgcgaag gaactcaaac atggttccgt ggaaaccgtt ggtcccggat ttcgtgacgg
     3301 cgttcatcac cgcgccgctg aagcggccgt actcggcgtc aaacgagttc gtgatgagcc
     3361 tgaactccgc caccgagtcg aggttcggaa ttaccgaagc gccgagattt cgcccttcgc
     3421 tgacgtcccc tccgttcaca aggaaggcat tggcggtttc ccgctggccg ttgaccgaga
     3481 cattgccggc cgataagcct cctgagacgg gccggtcctg ctgcatggaa ccggcggtgg
     3541 tcggcgccac gccggcctgc agtcccaaga ggtcaatgta actacggccg ttgagaggaa
     3601 gactcaacac tttcttctct tcgacgactt caccgagttg cgtgttcgtg gtttcgatct
     3661 ggagcgcgtt agcgctcact tcgatctgct gctcgatgtt acctacctgt agaaccacgt
     3721 ccacgcggcg ttgttcgttt acgctcaggt cgaacgcggt ggtcacgaac ttctgaaacc
     3781 cctgtgccgt ggcctccacg cgataccgtc cgaccgggag agccagaaga cggaactggc
     3841 cggctgaatc gcttgttgct tcctgccgct ggctggtccc gaggttgatt gcaacgaccc
     3901 gcacacctgg aaccaccgca gatgtggagt ccgtgacgag tccaagaatt gtgccggtaa
     3961 cgtcagccct caaaagagct ggcgctatca cgacagagag aactgcaagc gcaacgccga
     4021 tgcgtacacg catggcaccc tccgattccg atgtgtagtg ttagcccact gtaactggca
     4081 taattatgta tcaaagccgg agtgcgatca aatagttttt tcttaatctt atggctaact
     4141 ggagtttgca agtgcaaaca aagaaagcaa aagcgacaga tgaaggcggt ggaaaacagg
     4201 tccggtagtt agaacttccg ggaccactcg cggggccagc gttcgacgac gactttctcc
     4261 tgggtgaaga actcgaccgc gtcgcggccc tggccgtgga ggtcgccgaa gaagctttcg
     4321 cgggcgccgc tgaaggggaa cgccgccacg ggcgcggcca ccccgatgtt gatcccgatg
     4381 ttgccggcct cgacctcgtt gcggaacttg cgcgcggccg cgccgctcga cgtgaacaga
     4441 caggccatgt tgccgtaagt tccactattg acgaggccga tcgcctcatc gacggtttcg
     4501 acgtgcatga ggctcaatac gggaccgaag atctcagtgc gtgagatttc ggttccgggc
     4561 gcgatgttgt cgagcacagt cggccggacg aagaagccgt cctcatgacc ggagatcttc
     4621 gggccgcggc cgtcaaccac cgctcgggcg cccgcgtcga ggcctttctg aacgatcgtc
     4681 tcgatgcgcc gcttgctctc tgaggtgatg acggggccca tctgcacgcc ctcgtcgagg
     4741 ccattgccga ctttccgctt ctccgcctgt gagacgatcg catcggtgaa tggggcgcgg
     4801 gcctcgccca cagtcacggc gaacgaagcg gcgaggcagc gctggccggc gcagccgaag
     4861 gcgctgtcgg cagagatctg ggctgtcatc tccatgtcgg cgtcaggcag aacgacgatc
     4921 gggttcttgg cgccaccgtg acattgcgcg cgcttgccgt tggcgccggc ccgggcgtag
     4981 acatactttg ccactggcgt ggatccgacg aagctgacgg cgcggaccgc gggatggtcg
     5041 aggatggcgt caaccgtctc tttcgcgccg ttcacaaggc tcacgacgcc tttgggcagg
     5101 ccgatctgct caatcagccg gaagacgttg tgcatagtga gcggcacctt ctccgacggc
     5161 ttgaccagga atgcgttgcc acaggcgatc gcgtagggca ggaaccagaa cgggatcatg
     5221 ccggggaagt tgaacggcgc gatgacggcg gccactccga gcggctggcg gatcatgtac
     5281 tcgtcgatgc cgcgggcgat gtcttcggag aggtagcctt gcatgagtat cgggatgccg
     5341 caggcagtct cgacgttttc gatggcccgt tgcatctctc cgcgcgattc ggcgagggtt
     5401 tttccgcact ccaatgtgat ggtgctagac agatcctcga agtgcgtctc gagcagcccc
     5461 ttcagcttga agagcggctg gatccgctcc atgacaggta cgcggcgcca ttcgatggac
     5521 gcggcggccg cggcacgggc agcttcgtcg acttccgacg ccgtggagat cggcacgcgg
     5581 ccgagcgttt ccgcggtcgc ggggttgagg aagccgagag tctccgcagc cgaagaggcg
     5641 cgccattcgc cgccgatgaa gttaggcagg gtatgcaccg gggtagacat gataatttcg
     5701 ttcgtatact gattgaacca cagatcgcta tgacacgcgc ctttgttgtc tgctttgcat
     5761 gtttgtcggc ggcggcccag acgctggatc tacccctgac tctcgaagaa actgcaggcg
     5821 tggcccgagc ggcggagccc gtaacagcag gtgtgccgtt tcccaagggt tttctcaaag
     5881 acgtgggccg cctgcggctc tacgatgcgg gcggcaagcc cgtggcggcg tcgttccgcg
     5941 tggtgaaccg ctggtgggac gatgcgtcca cacaggtggc atctatccaa tgggcgcact
     6001 tcgatttcct ggcggacgcg ccggcgaacg gcaaggccgt ctaccggctt aggctgtcgg
     6061 atgaagaagc gcctgcgcca tcggcgccgc tgcgggtgga gacggacggc gaccgaatcc
     6121 gggtgaatac cggcgcgatc gcgttctcgg ttagccggag cggtccgttc ctggaggctc
     6181 cggggctgaa ggacgccgat atcctgctgc ggtccgacga gcggatctac aaagcgagca
     6241 acgggccggc gacggaactc gtggtcgaag agcaaaaccc gctgcgtgtg gtgctgaagc
     6301 gcacaggatc acacggctgg gtggacgggg aggaccgggc gctcgactac gtgatgcgaa
     6361 tcgtcgcgtg ggcggggcgg ccgcagattc gcatcgttta cagcttcgtg aaccggcaag
     6421 gccagcagat gagcgatttc gtgcggctgg acgggctgtg gctgcaatac cggctggagc
     6481 gtgcggctga accgcaggga atcgaacagc tttcggcgga accacggcgg cagggctggt
     6541 tcgaggccgg cggcatcggc gtagggctac gctggttctg gcagctttac ccgaaggggt
     6601 tcgaggtccg gcaggacggg accgtccggc tcgcgctgtt ccccgagacg gcgcggccgc
     6661 agaacatcta caagggcgtc gcgaagacgc acgagatgat tctgtcgttt ggtggggaga
     6721 acctctcggc acacctggat cacccgctgt atgcggtggc tccgccgaaa tggtacacgc
     6781 gggaaacgaa ggcgctggga cgtttggtcg agtcgtcacc ggaggcgtat cggccggaac
     6841 actggccgct ggtcgaacgg tatgaccggt ggctgacggc gtgtcgcgac gcggtgctcc
     6901 agaagcgcga ccgcggtttt cagtttcagg accggcggta cgacgagtac gggatgctca
     6961 acttcggcga cgcgatgcac aagctggtta cgaacgaccg gcggcccgac ttcgggatcc
     7021 actgggagac ggagtactac gatttcccgc acgcgctatt cctgcatttc tttcgaaccg
     7081 gagacatgaa gtcgttccag acggcggtgg aagcggcggc gcacctggcg gacgtggaca
     7141 tctcgcatga cgagacggtt cccaaacgga acggagcggc ccgaacgggg ccgggactca
     7201 accactggac gcggtactcg aacggcgtat tcatctcgtc gtcgagctgg gcgttctaca
     7261 aaaacgagag cctgttcgac cggtacctgc tgacggggga cctatggtcg cgggacgtgg
     7321 cgcggctgtc gtccagcttc ggcaccgagt acaacggtct ggacctgcaa agcaacacac
     7381 ggtcgatcgg gcatggcttg ttcgcgatgc tgaaggcgta cgaggtgttc ggcgacaaaa
     7441 agtacctgga ccgggcgcac tggattgtgg attgcgtgca ggcgtggcag gacggcgacg
     7501 tggcgaaact gaaagtgttg aaccagcggg cggtgtggga tccgcagttc aaaggtggat
     7561 acagccacca gtcctggatg tacgggatcg cgctggaagg aatggcgcag gcgtcgatga
     7621 cgctcgggcg tccggagatg ccgggctacc tgcggcgcgc cgcggactgg attttcgcca
     7681 acccgaagga gtgggaccca aaccgccgcc ggttcttgaa ctcggacacg ctggcaccga
     7741 tgctgacacc ggggctcgcg tacatcgccg agacgagcgg agaccgcaag tattgggaca
     7801 tcgcgttgga gaacttcacc cggcacgtgg aggaaggacg gaccacggac cggctcaagc
     7861 tgttcgcgca gttgttccgg aactcgcaac ggtttctgtg gtacttgtca gcggaggcgg
     7921 ggaaggaagc ggctggggcg tctggcagcg agtgaacgcg ggtacacgcg aatctggcgg
     7981 cccactccac agcgcgatcg agtgatacag tattgtctgc gcatggaagc cctccggccc
     8041 gagacgcccg atcaacttgc cgagtcgctg atcacggctg cgtccgcggg taggactatc
     8101 accctcggag gcgcgttcag caagaacggg atggcagggc cggtcaagcc gtccgacgtg
     8161 acgatttcga cgaccggcct gaaccggatc ctgcaatacg aaccgcgcga cctgaccatc
     8221 agcgtcgaag ccggcatacg atacgaagat ctcgcccggg cgctcgagga aaacggacaa
     8281 atcctgccgc ttgacccgcc attctcaagt gaagcgacga tcggcgggat tctggcggcg
     8341 aacacgtgcg gatcattacg gcggctctat ggcacggccc gggacctgct gatcggaatg
     8401 aagtttgcga cgctcgaggg caaactagtc cagtcgggcg gcatggtggt gaaaaatgtc
     8461 gccgggctgg acatgggtaa gctattgatc gggtcgttcg ggacgctggc ggccatcgcg
     8521 gtggccaatt tcaagctggt cccaaagccg ccgtgttccg gcaccttcct gctcgagttc
     8581 gaggacgtcg cccaggcgat ggagacccgc gaccgcattc tccacagcgt gcttcagccc
     8641 acggccctgg acctgctcaa cgccgccgcg gcggaacaac tcggccgcga gggatgtctg
     8701 atcgcactcc aagcgggcgg caacgctgcg gtgatggagc ggtaccgccg agaactcggc
     8761 gctgcacgcg cgcaggacga aggtgaagaa gccaatttct ggacaggcgt ccgtgagttc
     8821 acgccgcatt ttctgaagag gaacccggaa ggcgtgatcg tgcgggcctc gtgcacgctg
     8881 gcacaggttc gcgaggtgat ggaatcgttc accggtccgg ctgtggcgag ggcggcatcg
     8941 ggtgtctgtt acggttactt ctctgagaac ggggcagctt ccgaatggat ggagcgggct
     9001 acggtgaacg gatggcgccc ggtgatcgag ttcgcaccgg aatcgaagaa gaccggactt
     9061 gatctgtggc ctggaccggg aagcgatttc gatacgatga agaaggtcaa ggggatgttc
     9121 gatcccggga accttctgaa tcgtgggagg ctttatggcc gtctctgagg aagtacgcag
     9181 ggacgcgccg cgccagttcg atctcgacaa gtgtgtgcac tgcgggcttt gcctgaactc
     9241 ctgcccgact tatcgcgagc ttcacctgga gatggactcg ccgcgcggcc gcgtctacca
     9301 gatggtacag gtggccgggg gcgccatggc gatcgggccc tcgtacatcg agcacatcgg
     9361 cctgtgcctg gcctgccgtg cgtgcgaaac ggcgtgcccg tcgggagttc cgtacgggcg
     9421 cctggtggag gcagcgcgcg cggagatccg ggcaaggatg ccacgatccc cggcggtgga
     9481 agtactgcgc cggttcgtct tcgaacatct gcttcagtcc cccgctctgc ttcagattgc
     9541 aggggcgctg ctatacgcgt atgagagaac gggactccag agactggtac ggtcctcagg
     9601 tctcttgaag ctgttcgggg aactgggcgg tattgagcag ctcacgccgc acgcggagtg
     9661 gccgttcttc ttccggaaca tcgggcagac gtttccggcg gaaggcacgg cacggtaccg
     9721 cgtggcgttc catgcgggct gcatcgccaa cgtctgtttc gcgcggctca acgaagcgac
     9781 cgtgcgtgta ctccggaaaa acggctgtga ggtggtcatc ccggcggaac agaactgctg
     9841 cggggcgctg cacgtccact cgggtgaacg cgaggaggcg cggaagctag ccaggcgcaa
     9901 catcgacgcg tttctggcag gcgggtacga cgccatcatc acgaacgccg ccggctgcgg
     9961 ctcgacgctg aaggagtatc acgagctact cgaggacgat ccggactacc gcgagcgtgc
    10021 acaacgcttc gtcaagctcg tcaaggacgt gaatgagttc ctcgcgtcga ttgagctgaa
    10081 ccgggagatg ggatcggttc cggtaacggc cacgtaccag gattcctgcc accttgcgca
    10141 tggccagagg atacggatac caccgaggaa gctgctggaa gcggcgccgg gattgcgttt
    10201 cgtcgaaatg cccctctcgg atatctgctg tggaagcgcc ggcatttaca acgtggtgca
    10261 gcacgagatg gcgatggcga tcttgcggaa gaagatccag accgtgaatg gcaccggcgc
    10321 agacgttgtc ctgacggcaa accctggctg catgcttcaa ctacgcgccg gagtggaact
    10381 gtacggaaag ggacagcgcg tcgcgcacgt ggtggagatt ctcgacgagg cgtacagaaa
    10441 ttacagcgcg ggcaagtgag tgcgcgccta ccaggcgaag acgaagggta accgtggggg
    10501 ctaagacgca cagaacaccc tccgaggaac ggaggatgac ccccttcgcc gtagctaccg
    10561 cgcgtccgcc ggaatcccta cgttaccttc cgcccatctt tctcttgcgc cggcgcagca
    10621 aatagagggc gagcatcgcc ccagcggcaa tccaaagaac agcggagagg gtagtgttgt
    10681 ccatcagatc tgttgcctcc tccaaagaac tatactgcaa tcccctcggg aattaccact
    10741 attattcgac gggatcttcg cgtgaaatct gctaatttct gattgagccg ccccggccgg
    10801 aagttatgag accggggcag ctccccggtt gacgcaccca taaggcaggc gtcaagctcg
    10861 aacggtccgg tccggcggcc gcgaatggca cggagcggga cccggacaga tcttgtagtt
    10921 gagtccggct tatcgttctt gcactttccg agagcggcgg cgcagaaggt acagagtgag
    10981 tactcctccc gcggcgatcc acagaatagc ggccaatgtg gtgttatcca tcgtttgttc
    11041 ctcctttccc gctaaactga ggctgacttt gcggccagcc tctcctcctc cctctaacga
    11101 agctccacga cgacctgaaa cagcgaggcg gggagttgca cgcccgcagc cgccagcctc
    11161 tcgctgaact ttggccggat gccggtggcc cgaaagcgtc ctttgactct cccgtcgtct
    11221 gcgaggccgg tcttctcaaa cacgaacaga tcctgaatcg ttacctgctc cccctccatc
    11281 ccgcagcact cagtgatatg cgtcacccgg cgggagccgt cggtcattcg ggcgatctgt
    11341 acgaaaaggt tgatggcaga actcatctgc tggcggatgg cgcggatagg aatgttggca
    11401 ttcgccatgc cgaccatggt ttcgagccgg ctgatagcgt cgcggggcga gtttgcgtgg
    11461 actgtggtga gggagccgtc gtggccggtg ttcatagcct gcagcatgtc cagggcctcc
    11521 tctccacgca cctcaccgac gatgatgcgg tctgggcgca tgcgcagcgc attgatgacg
    11581 agctgcctga tcctgatcgc accttggcca tcaatgttgg ccggccgcgc ctccatccga
    11641 gcgacatgcc gctggcggag ctgcagttcg gcggcgtctt cgatggtaac gatccgttcg
    11701 tgttccggga tgtggccgga gaggatattg agcagagtcg tcttgccggc gccggttcca
    11761 ccggacacga tgatattcag ccgggccttg acgcaggcgc ggagcaactc tagcatgcct
    11821 tcggtcaagc tgagattctt gacgagcgcc tcggcgggca gaggactgtg gccaaagcga
    11881 cggatggaca gcagtggacc atccaccgcg accgggggca caacggcgtt aacacgggag
    11941 ccgtccgcaa ggcgcgcgtc gacgagaggg gatgtctggt cgagacgccg ccctactctc
    12001 gatacgatct tctccatcac tcgaaccaga tgagcgtcat cgcggaactg gacggccgtt
    12061 tcgtagagac ggcccgcacg ctcgatgtgg accattttcg gcgtagtcac aaggatgtca
    12121 gagatggttg ggtcctccag gaggggttcg atggggccaa gtccgtagac ctcctcgagt
    12181 acctggtcga tgacggtttc cctttcaccg ttgagaggca gccgcaggtc gtcgatggct
    12241 cttccgatcg aggcgcggac ctcggttcgg gcgcgctcgt tcaccatgtt gcccagttgg
    12301 tcgaggttca gggtatcgag caaccgagtg tggatggcga tcttcatctc gatcagattg
    12361 gcaggaagat cgtgcgggct aaccggcgac tttgaggccg cggcgccgac tcccagaata
    12421 tgggctctcg attcgacctg tgttattggg gctgctgcca ttgtggttcc tccagggctc
    12481 agggataaac tctagacgta ttatacgtac tatgaccgac cgtaagtcaa ggaaattctg
    12541 gtatttcctt gaaattttac gaaaaacgag gcgaatcaac cgagggatta caacaatagc
    12601 tctagtactt tagatatatt gaccgttctc tgagagggtc tggaaaaaaa caaacgcccc
    12661 gctgtctctt ccaacggggc gcctttgcaa agactgagtg atgccgtgct tactcggcca
    12721 gggactggtc gccgatgtct tcggagagtc cacctgtgta gtggaggcgg gtctcgtcct
    12781 cgtagccggg aatcccttcg agggggccct cgggttcctc agtcatgacc tcctcctcga
    12841 tgtcctcgcc ggcgatcttg acgcggcggt agtactccat gccggtacct gcggggatga
    12901 ggcggcccat gatgacgttc tctttgaggc cgcgaaggtg gtctaccttg ccgttgatgg
    12961 cggcttcggt gagcacgcgg gtggtctcct ggaacgaagc ggcggagata aacgagtcgg
    13021 tcgagagcga cgccttggta atcccaagga gaacggcctt cccgtcggcg ggacggccgc
    13081 cggcctcgac gacgcgaagg ttttcctctc gaaacttgaa cttgtcgacc acttcctcgg
    13141 ggaggaactc ggtgtcgccg atttcctcga tcttcaccca gcgcatcatc tgacgaacaa
    13201 tgacctcgag gtgcttgtcg ttaatgttga caccctgaag gcggtaaacc tcctggattt
    13261 cgttgaccag gtatttctgg agttccttct cgccgagaac gtgaagaatg tcatgcgggt
    13321 ttcgcggtcc gtccatcagc gggtcgccgg cacggacgcg ctcgccttcc tgcacgttga
    13381 tgtgaacgcc gcgcggaatg aggtactcgc gctgctgtcc gtcgtctccg acgacgtaga
    13441 ctttgcggaa gcccttggaa acctccccga acttgacggc gccgttgatc tcggaaatga
    13501 ctgccgtttc gcgaggcttg cgcgcttcga acaactcgac gacgcgcggg agaccaccgg
    13561 tgatgtcctt cgtcttcgtg gtggctcgcg ggatcttcgc aagcacgtcg ccggcgtgcg
    13621 cttcctcgcc gtcgcgcacc atcaggtggg catgggtcgg catgaggtag cgtttctcct
    13681 ccgaccgcgt gcctccggca gggcgaatca tgacggtagg catacgctta gcgtcaggtg
    13741 cgtccacgac gacccactgc gacatgccgg tgatttcgtc aacctgctcc tggagcgtct
    13801 cgccatccac gaggtcacgg aagtggactg tgccgcttac ttcggtaagg atcgagaagg
    13861 tgtagggatc ccactcaaga aggacctggt ttgcctcgac cgacgccccg tcctcgaaca
    13921 ggattttggc gccgtaaacg acttgatagc gttccttctc gcggcccttc tcgtcgacca
    13981 cggccagaat tccgttgcgg ttcatggcga tcaactcgcc gaggtggttt cgcacggtga
    14041 cgatgccgag gtacttgacc ttgccgcccg acttggcgtc ctgagtggat tgctcactga
    14101 cacgggttgc ggcgccgccg atgtggaagg tacgcatggt caactgcgtg ccgggctcgc
    14161 cgatcgactg ggcggcgatg acgccaaccg cttcaccccg ctccaccaga cgtccggtgg
    14221 ccaggttacg gccgtagcag agttggcagg cgccccgttt ggactcacag gtgagcacgg
    14281 aacggatctt gacgcgctcg atgccagagg cctggatcaa agcggccagc tcctcgctga
    14341 tctcctggtt tgcccgaatg agaatattct ggtcgtaatc gagcacgtcc tcgagcgcca
    14401 cgcggccgac gatgcggtcg cggaggggct cgatgatctc accagattcg acgatcggtt
    14461 cgacgaaaat gccgtcgttg gtgccgcagt cgaattcgct gacgatgacg tcctgggcga
    14521 cgtccacgag gcggcgcgta aggtaacccg agtcggccgt cttgagcgcc gtgtcggcga
    14581 gtcccttgcg ggcgccgtgg gtcgagatga agtactggag tacgttaagg ccctcgcgga
    14641 aattcgcggt gatcggggtc tccatgatct cgccggacgg cttggccatc aagccgcgca
    14701 tgccggcgag ctgtcggatc tgttgtttgg agccgcgggc gccggagtcg gccatgatgt
    14761 aaatggggtt cagatagcgt ccggtgcgat catcctcctc cattaccttg aacatttcat
    14821 cggagacacg ctcggtgact ttcgaccaga tttcgatgat cttgttgtag cgctcgccgt
    14881 gggtgatggc gccttcctgg tactgctgct gcacctgcat gacctgtttt tcggcgtctt
    14941 tgacaagctt ggctttctcg ccgggcacaa ccatatcgtc gatcccgatg gagatgccgg
    15001 cgcgggtggc atagaggaag cccaggtcct tgacccggtc gagcatctgc accgtggtcg
    15061 gcagcccaaa ccggaggtag cagtgctgga cgagttgtgt gaggcctttc ttcttaagca
    15121 ggccgttgat aaaaggcatc tcgtccggaa ggacgtcgtt caagatgacc cggccgaccg
    15181 ttgtgtccat gtactgcttg ttgaactcga tcggttcggt atgaaggacg ttttggttgt
    15241 cgaaggcctt ggtgaggtcg atgaccttcc cggagtaccg cagcttgatc ggcgtaagtg
    15301 tttccacctc gcccatctcg agcgcgatga gaacatcgtc attagaggcg aaagtacggc
    15361 cctcgccctt ggtcttcggt ctggccttgg tgaggtagta cagcccgagc accatgtcct
    15421 gggtcggtac ggcgattgga gacccgtgcg ctggcgagag aatgttgttc gaagccagca
    15481 tgagggtggc cgcctcgatc tgagcttcgg gcgacagagg aatatggacg gccatctggt
    15541 cgccgtcgaa gtcagcgttg aacgccgtac agacgagcgg atggatcttg atggctttgc
    15601 cttccaccaa cacgggctcg aacgcctgga tgccgagtcg gtgcagcgtc ggggcgcggt
    15661 tgaggaggat ggggtggtcg cgaataacct cttcgaggat gtcccagacc accggatcct
    15721 gctgttcgac cagttctttg gcctgcttga tggttgtgca gtggccgcgc tgttcgagcc
    15781 ggtgataaat aaacggctta aacagttcga gcgccatttt cttgggaagc ccgcactggt
    15841 ggagcttgag gtcggggccg acgacgatca ccgagcgtcc cgaatagtcg acgcgttttc
    15901 cgagcaggtt ctggcggaag cgtccctgct tccctttcaa cgtgtcggaa agcgacttga
    15961 ggggccggtt gttggcgccg cggagaacgc ggccgcgccg cccgttgtcg aacagggcgt
    16021 cgacggcctc ctggagcatc cgcttttcgt tccggacgat aacgtccggg gcatgcaact
    16081 cgatgagctt cttgagccgg ttattccggt tgatcacccg gcggtagagg tcgttcaggt
    16141 cggaggtggc gaagcgcccg ccgtcgagcg gaacgagcgg acggagttcg ggaggaatga
    16201 ccgggaggac atcgaggatc atccactcgg gtttgttccc cgacttgcgg aacgattcca
    16261 caacccggag gcgcttggcg aacttgagtt tcttctgctg ggacacctcg gtcttcatct
    16321 tttcccggat gtccaggctg agggactcga cgtcgatctt cttcagcagc tccttgattc
    16381 cttcggcgcc catcatggcg acgaatttgc cgggaaactc ctggtcgagc tgccgtttcc
    16441 gctcgtcgct aatcacttcg ccggtgctga gcgtggtgag ttcgccgggg tccacgacga
    16501 cgaaggcctc gaaatagagg acccgctcca gttcgcggag ggtgatgtcg agcagatagc
    16561 cgatccggct gggcaggccc ttgaaaaacc agacatgcga gcagggactg gccagctcga
    16621 tgtgtcccaa ccgctcgcgc cgtacgcgcg ccagcgtcac ttcgacgccg cacttgtcgc
    16681 agatgacgcc gcggtgcttc atccgtttgt acttgccgca caggcattcc cagtcctgcg
    16741 tagggccgaa gatgcgggcg caaaacaggc cgtcgcgctc cggcttgaag gtgcggtagt
    16801 tgatggtttc cggtttagtg acttcaccgt gcgaccaact gcggatcttt tccggagacg
    16861 cgagactgat gcgaatcgaa tcgaagtccg cgatcaggtt cgctctgtcg tacggagagg
    16921 atcggtacaa ggctgcctcc ttatcaactg aaccatcggc ccgtccgggt caacagctcg
    16981 atgaatcaat gcactagtct gccgccagcg cggtttccag caactcgcgc ggcttcttca
    17041 ttaactcgac gtccaggcag agcgactgga gttcgcggat gagcacgttg aacgattccg
    17101 ggacacccgg ctcagccgcc gcctcgccct tgacgatcgc ctcataaatc ttggctctgc
    17161 catagacgtc gtccgacttg gccgttagca gttcctggag gatgtaagcg gcgccgtacg
    17221 cctccagcgc ccacacctcc atctcgccga agcgctggcc gccaaactgg gccttgccgc
    17281 ccagcggctg ctgtgtgatg agcgaatacg gaccgatgga tcgcgcgtgg atcttgtcgt
    17341 cgaccaggtg cgagagtttt agcatgtaga tgtaccccac ggtcaccggc tgctcgaacg
    17401 ggagcccgct catgccgtcg cgcaatgtga tctttcccga agtcggcagg ttggcctcaa
    17461 ccaattcctt cttaatgtca cgctcggtgg cgccgtcgaa caccggggtc gcataaacgc
    17521 gattgagcgc gtgagccgcc cagccaaggt gagtttccag aatctggccg acgttcatgc
    17581 gggaggggac gccgagcggg ttcaggatga tctccaccgg ggtaccgtca ggtaggtggg
    17641 gcatgtcctc gtcgggaacg atcctcgcaa tgacaccttt gttgccgtgc cggccggcca
    17701 ttttgtcgcc gaccgacagc ttgcgcttca tcgcgatgta gaccttgacc agcttgatca
    17761 cgcctggagg aagctcatcg cctttcttga gcttggcggt cttctcctcg gcgatctttt
    17821 ccagcacggc gacctggcgg gaggtcatct cctcgatctg atcgatctcg tcattcagcc
    17881 tcgggtcctt gtccttcagc ttcattcgct tcaggtcgcg ggacctgagc ttttcgatga
    17941 cgtcgcgttc gagcgccatt cccttcacca gcaggcgctt gttagttttt tcgtcgtgca
    18001 ggtcggcgag caggagtttg ccttccagca gcccggcgag ccgcttggcg cgctcgtcgt
    18061 tcagaatccg cttctcgtct tcgaggttgc gatgcagccg ggcgatctcg tcatcgagaa
    18121 tctgcttcga gcggtcgtcc agctccgcgc ccttccgcga gaagatcttg cagtcgacca
    18181 ccgtgccctc gatgcccggc gggcagtaca gagaggcatc ctttacgtcg ccggccttct
    18241 caccgaagat ggcccgcaag agcttctcct cgggcgtgag ttgtgtttca cccttgggcg
    18301 tcaccttacc cacgaggatg tcgcccggct tcaccgtggc gccgatgcga atgacgccgc
    18361 tctcatcgag attccgcagg aaactttcgg cgatgttcgg gatgtcccgg gtgatctctt
    18421 cggggccaag cttggtatcg cgcgcttcga tctcgaactc ctcgatgtga atcgaggtgt
    18481 agtaatcctc cttcaccagc ttctcgctga cgatgaccgc gtcctcgaag ttgtagcctc
    18541 gccagggcat gaaggcgacg agtacgttgc gtccgagggc gagctcgccg ttatcggtac
    18601 acggaccgtc ggcaagcaca tcgcccttct tgacgcgctg gccatgactg acgatcggct
    18661 tctggctgat acaggtgttc tgattcgagc gcttgaattt cgtgagctga tagatgtccg
    18721 caccgacctc acgggacatc tgaccctcgt gggcgccgcc ctcgacgcga acgatgatcc
    18781 gctcggagtc caccgaatcc accacgcccg cgcgcttgca gattctcacg gcgcccgagt
    18841 cgcgggcggt cacgcgctcc attccagtgc cgacgtaagg ggcctcggcg cgaagcaatg
    18901 ggaccgcctg gcgctgcatg ttcgagccca tgagcgcgcg gttcgcgtcg tcgttttcga
    18961 ggaatgggat caggctggcg gccaccgata cgagctgttt tgggctgacg tccatgtact
    19021 cgacggcgtc gcgttccatg agcagaaagt tgcccgcctg ccgggcattg accagctcgg
    19081 cggtgagtcg cgattccgaa tcgagctcga cgttggcctg agcgatcgtg tacttgtcct
    19141 cctcccatgc agagaggtac gtgcagtagg cctcatattc ggccggctgc tttttggcgc
    19201 tgagcgcacg gttggcgcgt tccacgtcgt tccgaaagac aatgtcgccg acgcggtggt
    19261 tcgtgtctcc cgggttgaga atcttgacct cgtcgatcac ctgactgtcc aggacgcggc
    19321 gatacgggct ctcgatgaag ccatagtcgt tgattcgggc aaaacaggac agagaagaga
    19381 tgagaccgat gttggggcct tccggcgtct cgatggggca aatccggccg tagtgggtcg
    19441 gatggacgtc gcggacctcg aatcccgcgc gctcgcgtga cagaccaccc ggacccaggg
    19501 cggagagccg ccgcttgtgc gtgatctccg acagagggtt ggtctggtcc atgaattgcg
    19561 agagctggct ggagccaaag aactcgcgga tcgcggccat caccggtttc gcgttcacca
    19621 ggtcgtgcgg catcgcggtc gacatttcct ggtagaccga catcttttcc ttgatggcgc
    19681 gttccatccg gaccaaaccg atgcggaact ggttctccag gagttcgccg accgcacgca
    19741 cgcggcggtt gccgaggtgg tcgatgtcgt ccacggagcc gatgccccgg cgcagccgaa
    19801 ggacgtagcg aatggtttcg ttgaagtcct cggcggagag ggtgcgctgg tcgaggggcg
    19861 ttgcgtctgc cttcccgtac agcttgatgt tgaacttcat gcgccccacc cgcgaaaagt
    19921 catacttccg cgggtcgaag aacataccgg agaagagctg ttgggcggta tcgagagtcg
    19981 gagggtcgcc gggccgcagt ttcctgtaga tctccagcag ggcgtcgttc tggctcctga
    20041 cgctgtcctt cctgagtgtc attgagatga cgttgccgac ttcgtcgcgc tccgggaaga
    20101 acacctcaaa gctcggcgtg ccgccctcga tcaatttcga aaccgaggcg gtggtgagct
    20161 cctgattcgc ctccatcaaa acctcgcccg tcgacatgtc aaccacgtcg gcgacgatgt
    20221 acgcgccttc gaggtcattg ggcgcaacct cgacatgctc gattttcgcc ttctggagtt
    20281 ccttgagtac cgcggtggtg atcttctttc ctgcagcaac gacggtatcg ccgcctcgag
    20341 tggtgaccgc gtgggagagc ttcaggccga ccaagcccgg ggataccgtc cagaacaacc
    20401 gattttcgcg gatggtgatc ttgtcaactt tgtagaaggt tcgcaggatc tgctcatcgg
    20461 acttcagtcc tagcgcccgg aggaacacgg tgccataaaa cttgcgcttg cggtcgatgc
    20521 gaacatgaag gaggttcttg gcatcgtact cgaactccac ccagcttccg cggtagggga
    20581 tgatcttgcc gaggaaataa ccctgcgccg gaatcttctc gaagaagacc ccaggggagc
    20641 ggtgtaactg ggagacgatg acgcgctcgg ttccgttgat gatgaaggtg ccgttgtcgg
    20701 tcatcagcgg gatttccccg aagaagacct cctgctcctt gatgtcccgg acggtcttct
    20761 gtccggtctc gccttccttg tcgtaaaccg tgaggcgaat ggttactttc agcggtgcgg
    20821 cgtaggccat gccgcgttcc tcgcactcgg ggacttcgta cttcagttgc agccccacag
    20881 ggtcgccgca acggttacag aacgtgacga tgttcttgtt gaacgtgccg cacttggggc
    20941 aaagcacgtc gcccgcgtgg aaggggtccg ttcgaatata tgcgccgcaa ccgggattcc
    21001 ggcaggtggt gcggaggtgg ttgaggccct tcagatttcc gcacttgcac tcccaattgc
    21061 cgatcgagta gtccacgaac tcgagctggc tcagcccgcg aaagtcggtg atcggaaaga
    21121 cggagttgaa tacgctctgc aatccgacgt cttcccgctc gtccgggagc aggtccatct
    21181 gcagaaaccg ctcataggac ttcttctgga cctcgatgag gttcggaatt gggatcgagg
    21241 tcttgatgtt cgaaaaatca attcgttcgc ggttggcgct actagcagga ttctgcattt
    21301 tgcactccta aaagccagac ggcaatgacc cgaaggtcca agttcaagca ctagcggccg
    21361 cgggcgggct agaacgcggg gatgacgggc tgcccacccg atctatatag cccataagaa
    21421 cgccgcgctg cgcgcgcggc ggggcaaact ggaactcttg tgatatgtga gacgagcggt
    21481 aatacggacc ggagtgaagg cgcacgtaca cctcgtgcga cgagggtacg gacgcaccca
    21541 gcccagctcc agacaaacta ctgccgctta cgcagcaaga gtgattcaca ggcgatagtc
    21601 cgggctcgac tccggttgag acatctaaca gtctagcaga cgtcgccagt gcggtcaaat
    21661 gccccaccaa ggcccgctgc caagtacctg gagtggaacg acttgtccac cgggtccttg
    21721 cgcgggccgc ggattgccgg ttaggagccc tacttcacct cgaccgtggc gccggcctcg
    21781 acgaacttct tcttgatcgt ctcggcttct tcctttgaaa tgccttcctt gacgggctta
    21841 ggagcgccgt ccacgaggtc cttggcctcc ttcaggccga ggctggtgac ttcgcgaaca
    21901 gccttgatga cgttgatctt gttcgcgccg gccgccgtta aaaccaccgt gaactcggtc
    21961 ttctcctccg ccgcgggcgc agccgccccg ccaccc
//
LOCUS       DATIFQ010000095        36478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2520840, whole genome shotgun sequence.
ACCESSION   DATIFQ010000095 DATIFQ010000000
VERSION     DATIFQ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 36478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36478
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520840"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..624
                     /locus_tag="VLE22_04225"
     CDS             121..624
                     /locus_tag="VLE22_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSB13640.1"
                     /translation="MLRTAKRNRPATVGPPGTYASSEVSRLARVSLRQLQWWDEKHVV
                     SPRHQGHKRMYTPEEVLEITVIAELRRKGFSLQKLRRVLKYLQRDMGRRLSDVLHNDS
                     DLHLLTDGKAIFLEDRRDRVIDVLKNARQPMFLVCVSDHARRLGSAPKKTVRSETIAA
                     GRKSRAV"
     gene            692..2287
                     /locus_tag="VLE22_04230"
     CDS             692..2287
                     /locus_tag="VLE22_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HSB13641.1"
                     /translation="MTSRRRHLFFVPLIVVLSTLLGGVFGPGLTGVSAASPQEQDEIQ
                     ASVRSFSRLYAVVEQNFAEPLDPDKAVYNGAIPGMLRTLDPHSSFFDPRYFQLLREEQ
                     KGHYYGVGMKVGPRNNKTIVIEVFAGAPAYKSGLRPGDVIIEVNGKSTEGLNTTEVAD
                     LLKGPRGTAAVVRIMRAGNEQPLVFNIIRDEISRKSVPDAYWVRPGIAYMHIQQFNET
                     TSREVDENLRTLGEESVKGLILDLRGNPGGLLNEGVAVADRFLRKGQTIVSHRGRASA
                     EKPYVARHGNGGRDYPIVVLVNRQSASAAEIVAGALQDHDRGWVLGDNTFGKGLVQTV
                     YPLSENTGLALTTAKYYTPSGRLIQRDYSSRSFFDYYYRKNTDTKNPLDAKMTDSGRT
                     VYGGGGIAPDEKFVPPRLNRFQMELYKDYAFFNFTSKYLGTRDIKVTTDWEPGQSVMA
                     EFRQFLIDESVSFNDVEFAQNEAWVKSQLKREMYASALSIDAANRIAIESDPAVEKAV
                     QSLPKARALLETAKKLIAQRSAK"
     gene            2334..4130
                     /gene="guaA"
                     /locus_tag="VLE22_04235"
     CDS             2334..4130
                     /gene="guaA"
                     /locus_tag="VLE22_04235"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HSB13642.1"
                     /translation="MVSSFVMTSYIPEPQVTVLDTGGQYCHLIARKVRELGVYAEVRA
                     SETPANTLRRRKAIIISGGPNSVYEAGSPTVDAAIFSLGTPVLGICYGQQLMAHLLGG
                     QVRKGDKGEYGLALLEMGAREGLFQGLSGSQQIWMSHRDTVAEVPPGFRVTGHTGTCA
                     VASMSDGSRRLYGVQFHPEVVHTSRGTEILSNFLFRISECERDWNPKHRLPLIEDRIR
                     EVVGNRNVFFFVSGGVDSTVAFTLCVRALGHERARGIYVDTGLMREGETEFVRSVFEK
                     LDSGAFEVCTAQEQFLSALQGVHDPEQKRQIIGEEFVRIQDRIIDAHHFLDEHWILGQ
                     GTIYPDTIESGGTEKAAVIKTHHNRVPGIRRLLEANRIVEPLSFFYKDEVREVGRELG
                     LPEEMLDRHPFPGPGLAIRCLCSETEAPVREVEDGWIVPVRSVGVQGDSRSYAPVLAL
                     HRSPSSREDLHTIATEMINRLPGVNRIVAYVAGHSSLARLRVAASWLSPERLARLRRV
                     DEIVRRLSHDSGFDRTVWQFPVILIPAGADGRPDSIVLRPVHSVDGMTARSVAMDPVL
                     LEKIATRLLDVAGVCAVFFDLTHKPPATIEWE"
     gene            4189..4923
                     /locus_tag="VLE22_04240"
     CDS             4189..4923
                     /locus_tag="VLE22_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HSB13643.1"
                     /translation="MTEPQVSLEGMGELKRLAGVFWEPKPVFEELAARPRWWVPLIFL
                     TVLALVYIGTFSRVIGWDTFIRQQFETNPRLQSLSSDQRAQAMEMQMKIGSRFAYVGG
                     ALGMTVSSLVIAGILFGAFNLLGGASLKFKQAFSITCYSLLPTALSSILALIVMLFKN
                     PEDFDLQNPLVLNVGAFLDPAATPKALHSLATSLDLFSIWVILLLALGFSVAARRISY
                     AKSLILVLVPWALYVAGKSGWAAMFG"
     gene            complement(4941..6935)
                     /gene="mutL"
                     /locus_tag="VLE22_04245"
     CDS             complement(4941..6935)
                     /gene="mutL"
                     /locus_tag="VLE22_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HSB13644.1"
                     /translation="MGRIRVLSDQVANKIAAGEVVERPASVVKELLENSLDAGSTEVR
                     IEVDSGGRRLIRVSDDGSGMLRDDAMLAFERHATSKLSDVKDLLSIATLGFRGEALPS
                     IASVSRLVLETRSAEETTGTRIEIAGGKMLRVEEAALAGGTAITARDLFYNVPARKKF
                     LRSEQTELAHIASLVTHYSLAHPGKTFLLRSGGDDLLYVTPVPTLKERVYQVFGSQTL
                     DELVDLSIRERHLAIPAPFVPPGAPADETEEPPGRTFRLSGFVSRPQVQKLNRNSIFL
                     FVNGRLIRDRLLMHSISSAYHNLIPPACYPFALLFLECDFEEVDVNVHPSKTEVRFRH
                     GSFVHDFVRDSIRETLMESRPISSLPLPQHSPDLPGGQAAAGLPYSEFTQRIENQVWS
                     EPPSQVVSGAVPEAPEFLPDVTLRAPQPPEARFDFGGAPFPVREPGVPEPDVSPMRVR
                     IPDTHGGFPAEAMADSSGSLAVLADLRPLGQIHDSFIIAAGRDGLWIIDQHVAHERIL
                     FEKVLRQSASGRVEMQRLLLPLILQLTPAQQIEYARIADELHHVGFETEPFGHRTIAV
                     KASPAGVAQADVERVVFEILETAEKELRGVSMDDLRRAMAASIACRAAIKMNMRLDQS
                     KMEWLLSALAATECPMSCPHGRPIALRYSTRDILRGFHRI"
     gene            complement(7002..8645)
                     /locus_tag="VLE22_04250"
     CDS             complement(7002..8645)
                     /locus_tag="VLE22_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB13645.1"
                     /translation="MRILTFLAGLLCAGYLYSQEVPQFPSPAYFRQTFSTPIPRVELQ
                     PPTRLEDFAVGDKLELSLRAYLELVLANNTDVAIQKVTVEVQRNAITRAFGRFDPTLQ
                     ASFNATRANSPASDVLAGAATVSQLSQPANFNYQQTLESGTTYNVGFNASKLSTNSSF
                     ATFNPSLTTRLTFGFAQPLLRNRGTYITRLPVLIARSRLRASEYGMRDQLLRLLTTAE
                     SAYWDVVEARENLKVQEQALALRGVALKRAQDELRLGALPPLDIFQPQADYAAAEIQV
                     SQARFRLAQTEDALRKQIGADLDPHVRNVPLELTETVLPPADTVSFDREAIVEKALQM
                     RPDLRSVLQNLETDDLSIRSATNNLRPDLSLTGSYASAGRGGNFFQRPISLGDTTTAA
                     TMIPGGFGDALDQLFGFNFPTYAFGLTLRLPLRDRAAAADFADAVVQKRLNSLRARTA
                     EQSIRLEVLNAISQVESSKDAVRLAVVARDLAQKNYEAEQKKYDLGTTVLFFVLDAQN
                     RLTVAESRLLTESINYRRNLLNLLRVNGSLLEERGVRIQ"
     gene            complement(8657..9877)
                     /locus_tag="VLE22_04255"
     CDS             complement(8657..9877)
                     /locus_tag="VLE22_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB13646.1"
                     /translation="MGLSSLLVHKLRSLLTMLGMIFGVGAVVAMLSITAGAQKEMISF
                     IDLLGVNNIIIEAKEAVDRNELQTRRATSAGLTFRDFRAISQNVQGIEAATPRKRFKP
                     GKVLPKTVNEPPVLIGVLPNFLEINSLKVIEGRFFTAEDDLRSAPVCVLGESAKVNLL
                     GYDKAVGKYVKINEVWLQVIGVLAHQATADTEVEGLEVVNRNNLAIAPLNTVMRRFED
                     GNSYLKDEIDGIYVRVTPQTDSIEVASVISAILSATHKDAGDFTVVVPAGLLEERRRT
                     QFIFEVVMICIAGISLLVGGIGIMNIMLATVLERTREIGIRRAIGARQADIVRQFLTE
                     AVMISIVGGLIGIAFGFSLSRVIAAAAGWSTVVTAGSVAVAFGVSVFIGLLFGIYPAV
                     QAAKLDPIEAIRYE"
     gene            complement(9945..11870)
                     /locus_tag="VLE22_04260"
     CDS             complement(9945..11870)
                     /locus_tag="VLE22_04260"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB13647.1"
                     /translation="MKKLMFRSLILIAVLAAAWGGYHFVRQLPQRQQQIPTARVRQGD
                     VMIRAFSRGELRAVRSVTLTAPNLFGTVQVTRLAPLGAMAREKDLIVEFDDSEVRARL
                     EEKQLELEQVDEQIKKAEAELAIRSNQDQVELLRARYSVRRAELEKSRNELLSKIDAR
                     KNELNLEEAQRRLKQLESDIKSRLEQAKAEIAVLREQRNKAVIELTREKSRLSQTRLL
                     SPISGLVFIHQNRFGGGRMFGMQVPDIREGDQVQPGMPVADVLDLSEMEVIAKIGEID
                     RANLREGQAVSMQPDALAGTRLRGQIKSMSGTASANIWSGDPSKKFDVLFSVDMKQLL
                     TTVGATPEQIQRVLATAERNRNKPPAASRPAFAMGGFPGGSPGGRRGSGGPGQESGGE
                     PGMLLAGIGSGGPSGGPGGEGQGATGERRSRGEGQGRRSQMSDADRQKMREALQKELG
                     DRKMEDLSQEERQKIFAKVRQSMPGASGQRREGLGQAGAQTAGGPSSSGIGPGMPFVG
                     GPPSGFTEKELADAQLPPPPEEDSQLEVLLRPGLLADVEITIEKIPNAIYIPAQAVFE
                     KDGKPIVYVQSGRRFEERSVTLLRRSEATMVVSSGLKPGEHVALADPNAKKGDKKSGQ
                     PESRPGQTGGATGAGKS"
     gene            complement(11893..13197)
                     /locus_tag="VLE22_04265"
     CDS             complement(11893..13197)
                     /locus_tag="VLE22_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB13648.1"
                     /translation="MSKDLKPKQRIGRPPASRKKKIILWTLSLVGFGGAGYAAYKYTS
                     PEVVEVPVAKVRRGEFVLAVRTRGEVKSTRSLTLTAPQVPNPQIVKLAESGKPVKKGD
                     VVVEFDTATQEQYYLERATTVRTVDSQIVQTQAQHRMTNEMDGMNLMQSEYNLERAKL
                     EASKAEILSEIEGAKNRINVGIAGGELGQVKTTINSHKVSQEADLARLGETKDKTIRD
                     MTRAKGYLQKMVILAPNDGIINILPNFRSGGTFGQMPPPFKEGDRAWTGAAIAEIPDL
                     SEMRAELKLDEVDRGKIRLGQLVKIRVDAIPDKEFTAEIDWISPIASLVWKGFMNADK
                     VFPTFATLKNLDPRLRPGMSASVDIIIESEPNVLLIPARASFVHDGKPSVYVQRGQRF
                     PVRVIQVGKRNDNDIVVLAGLKEGEMVALEDPAEAAKKAKKR"
     gene            complement(13217..14209)
                     /locus_tag="VLE22_04270"
     CDS             complement(13217..14209)
                     /locus_tag="VLE22_04270"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HSB13649.1"
                     /translation="MKKGDVVAGFDRQDMENRLDDFEAGVRQAEASMKRFQAELEVAR
                     KAHEHSIRVAKSELDKARLDLKTIPVRSTIQTETLRLAAEEAEASYEQILKEAKYLEA
                     SLRAQLRVAELSLAEAKVETQRVERDVDRMLMKAPIDGLVVMQTTWRGGEMGQIQLGD
                     QVHPGQPFMRIVDPDSMVVSASVNQVDAEMLRIGAHARVRFDAYPDLELPARVYSIGA
                     ITRASGFRADFVKEIPVTLRLERMDPRVIPDLSVSVDVVVNSEADAIIAPRESVFQTG
                     GGEKPYVFVQTKSGWQRREVELGRASHTTVAVRDGLRAGEVVALERPPSTPKKV"
     gene            14307..14471
                     /locus_tag="VLE22_04275"
     CDS             14307..14471
                     /locus_tag="VLE22_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13650.1"
                     /translation="MDPSPEDPRALELLLDEVERDELLLEEEVWLPNRVVAARKAEAV
                     DLPAPVPPLA"
     gene            complement(15135..16571)
                     /gene="rho"
                     /locus_tag="VLE22_04280"
     CDS             complement(15135..16571)
                     /gene="rho"
                     /locus_tag="VLE22_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HSB13651.1"
                     /translation="MANELENQENRETVAADEAKAPEQPAAAPPPPPTTTPPTPPTRK
                     KQGHGGHGPGQQAKNGSTTLDLVELKEMSIQKLNQIAKDFGVQGAAGLRKQELIFKIL
                     QAQAEKSGLIFSEGVLECLPDGFGFLRAPEYNYLPGPDDVYVSPSQIRRFDLRTGDTI
                     SGQIRPPKEGERYFALIKVDAINFEPPEEARNKIFFDNLTPLYPNERLRMETVKDNFS
                     GRVMDLLTPVGKGQRGLIVSPPRTGKTMLLQSIANSVAVNHPEVALIVLLIDERPEEV
                     TDMQRSVKGEVISSTFDEPATRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAY
                     NTVVPPSGKVLSGGVDSNALQRPKRFFGAARNIEEGGSLTIIATSLIDTGSRMDDVIF
                     EEFKGTGNMEIHLDRKLVDKRIFPAIDINKSGTRKEELLQPKEELNRVWVLRKVLSPL
                     SPVECMELLLDKLGKTRSNAEFLAAMSG"
     gene            complement(16718..19135)
                     /gene="lon"
                     /locus_tag="VLE22_04285"
     CDS             complement(16718..19135)
                     /gene="lon"
                     /locus_tag="VLE22_04285"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HSB13652.1"
                     /translation="MLTSKERSETKRLPMMPVRDVVIFPHMMTPFVVGRESSVRALEE
                     ALAGEKKIFLATQHDASVDEPRPDEIYSVGTIVNIVQSLKLPDGNIKVLVEGVERAKI
                     VSVGEDEGYFRAVVKTFGFKVEAGPQIDALTGRVTTLFEQYVKLSQNLNYETMIAAIR
                     VDDPGRLADTVGANLQLTIEEKQELLEIFDPVERLTRVAEMLDIEIEKLNVDRTIQGR
                     VKRQMERAQKEYYLNEKIKAIQKELGRGEKSEFDELKKKIESAGMTKDAHEKAMAELR
                     RLENMPPMSAESTVSRNYLDWLLAVPWKKKTKEIRDLKFAEKVLESDHYGLEKIKERI
                     LEFLAVRRLVKHPKGSILCFVGPPGVGKTSLGMSIARATGRKFVRMSLGGVRDEAEIR
                     GHRRTYIGALPGQIIQMMKKAGTRNPVFMLDEVDKMSMDFRGDPSAALLEVLDPEQNF
                     MFVDHYLDVEYDLSQVFFIATANVLHTVPPALQDRMEVIRLSGYTELEKLEIAKRFLV
                     KKQLEQGGLGDNQIRFTDEGLLTLVRSYTREAGVRNLEREIGNLCRKVARSLVTSQTA
                     DAKAEFPLVTITKETIPGFLGPLKFRDLEADKKNEVGAATGLAWTEVGGQILTTEATI
                     MEGRGKLTTTGKLGDVMQESAQAAMSYIRSRAHSIGLPKDFYRHLDIHVHVPEGAIPK
                     DGPSAGITIATTIVSALAKVPVRCDVAMTGEITLRGRVFPIGGLKEKLLAAHRHGIFE
                     VILPKDNEKDLPDIPEVIRKDMRLHFVESMDEVLKVALEREIEALPIAPGPSTVEIAS
                     QHDEKLH"
     gene            complement(19259..20527)
                     /gene="clpX"
                     /locus_tag="VLE22_04290"
     CDS             complement(19259..20527)
                     /gene="clpX"
                     /locus_tag="VLE22_04290"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HSB13653.1"
                     /translation="MKRTGSDDSLRCSFCHKSQDVVGKLISSPSDYPRAYICDECIAV
                     CNSILEDDRNEQPRASAHKLPKPLELKEYLDQYVIGQEASKKKLAVAVYNHYKRVQMN
                     KHRTTDVELSKSNILLIGPTGTGKTLLAQTLARILDVPFAIVDATTLTEAGYVGEDVE
                     NIILRLLQNSDWDVQRCQQGIIYIDEIDKIGRKDENPSITRDVSGEGVQQALLKILEG
                     TIANVPPQGGRKHPHQEFTPVETSNILFICGGAFVGLEKVIGRRVGRKAMGFMTAEDA
                     PEGARRGNSRRDTNLLQEIQPVDLIRFGFIPEFVGRLPVVSVLEDLDKDALVQILTKP
                     KNAIVRQYQRLFEFENVRLKLSDDALEAIASLALERKVGARGLRMILEDLMLDLMYYL
                     PSYKKVREFLVTKEMVMTQKINLAVLEKAG"
     gene            complement(20662..21927)
                     /gene="tig"
                     /locus_tag="VLE22_04295"
     CDS             complement(20662..21927)
                     /gene="tig"
                     /locus_tag="VLE22_04295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HSB13654.1"
                     /translation="MASQESCKQTLEITIPIEDVEKETNRVVESLSQRVRLPGFRPGK
                     VPVGIIRTRFSSDIREEVIRNLVPKRFQKEVEDRNLRVVGTPDITDVHFHSGEPLRFK
                     AEFEVSPEFELQDYRGLTVAYQDPEVSDDDVARRIEELREQKADYVNVDPRPAEDGDH
                     AVVALEALSGLEKPLKQDEVMLHVGGQDTLDAFTENLRGMSPGDEKEFDVRYPEDFGD
                     AKLAGKSVRFRAAMKGIRRKELPEINDEFAKDLGDYLTMDELRSEVRKSLLREKEYLA
                     QQEAKNKLVEKLVEMHEFPVPEAFLDRQIENQVEQYLRAVAASGGDPRSVKLDWQKVR
                     ESQREQATKDVKASLLLERIADREAIEVMQDEVDREVQRIARQDREPVAAVRRRLEEQ
                     GVLRRIASRIRTEKTLTFLFEHARKVAED"
     gene            complement(22006..23286)
                     /gene="ahcY"
                     /locus_tag="VLE22_04300"
     CDS             complement(22006..23286)
                     /gene="ahcY"
                     /locus_tag="VLE22_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSB13655.1"
                     /translation="MSTNTQTTTGCDVRDLSLAELGKRRTEWAFQSMPVLQTIRKQFI
                     KTQPLAGIRIAACLHVTTETANLMVTLRDGGASVSLCASNPLSTQDDVAATLVRDYNI
                     PVFAIKGESNDVYYQHIASVLDHKPQITMDDGADLVTILHTKRKELLDVVIGGTEETT
                     TGVIRLRAMANEGVLRYPIVAVNDAHTKHLFDNRYGTGQSTIDGIIRATNVLMAGLKV
                     VVAGYGWCGRGVAMRARGMGANVIVCEVDPIKALEALMDGYRVMSMSDAAETGDVFVT
                     VTGNKSVMGREHFDKLKNGAILANSGHFNVEIDLEALGRMASSRRPTREFVEEFVMRD
                     GRRIYVLGEGRLVNLAAAEGHPASVMDMSFANQALSAEFMVREHKSLEKRVYSVPETI
                     DREVARMKLDAMGVKVDRLTPEQEKYLASWSEGT"
     gene            complement(23367..24530)
                     /gene="metK"
                     /locus_tag="VLE22_04305"
     CDS             complement(23367..24530)
                     /gene="metK"
                     /locus_tag="VLE22_04305"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HSB13656.1"
                     /translation="MTENKHLFTSESVTEGHPDKMADQISDAVLDAVLAQDPTGRVAC
                     EVLVTTGLCVVSGEITTSCYVDVPKVTRSVVNEIGYNDATFGFDGKTCGVINTIQSQS
                     PDIAMGVDTGGAGDQGLMFGYACDETPELMPLPIMMAHKLVRRLSEARRAGELDFLRP
                     DGKSQVSVEYVDGKPTRIDAVVVSTQHSDKVPTEVLRAEVRRRIIDPVLPPSMVDAAT
                     KYHINPTGRFVIGGPHGDTGLTGRKIIVDTYGGMGRHGGGCFSGKDPTKVDRSACYMA
                     RYIAKNIVAAGLAKRCEVQLAYAIGVADPVSVMVNTFGTGAASEDRLVELVRANFPLT
                     PRGMIEHLRLRRPIYRKTAAFGHFGRTEETFSWEAADKAEALRSDIGVALARG"
     gene            24833..27217
                     /locus_tag="VLE22_04310"
     CDS             24833..27217
                     /locus_tag="VLE22_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB13657.1"
                     /translation="MLLFGLRDDLREAFHRIRRNPRTSLVAVGTLALGIGAATAILTV
                     AHTCLMAPPPFREPDRIVRVHGPRTSWGWQGVSGADFLDYLEEPGLFQSGTLVGYAEF
                     SWTGQSLPGFDGAEVLRGYVVTADYFRVMDHPMAAGRGFLSGEEHAVVISHALWQRRF
                     AGRGDIIGQAVTINGTSHVVVGVAGRGFLTYGSYEVMAWVPISPDREWRSSRQYSCFA
                     RLAPGIGLEQVQQRLDVLNRHLAEAYPATNAKYAAKVEPFLHEVRQEVRPAMLALLAG
                     VLCLLLIVVANVASLLLARAAGQAREMAIRTALGASRLRLYRMVIAESCLLALVASVG
                     GVALGAWLITGLRELIPATLAVGWAFALDARVFAAAFLLSALAGLTAGVAPAFESFRM
                     AAGGIRPTISRSRLLRGITTVEIALAVVLAIGAGLLGKSFLQLLDRPLGYRTDSLLGM
                     RVRLVGDRYKSIDQRAAYWSELMERASTLPGIAKSAMVSDLPMGWQYSGGSFEAADKP
                     VAPGETRPRAHQIVASPGYFATLGIPLLAGRGFNEGDGPKSEPVVIVNDFLAQKIWPG
                     ENAIGKRIKAWDGKTWRRVVGVVYRVRHGGPADQYENQLYVPYRQGNSNVMFLVVRTH
                     VPPESVVPAIRTTLKFMDPNVPAFEIRSMKAAFERETALPRLPMALTVGFAGLAALLA
                     ALGLFGVIAYWVSRRTKELGIRAAIGARPAELRALVLRQGLRMAAIGLTAGVAVSLTV
                     MRYLHSLLYGMSERDPWIYAGAIALALGTAIFACWLPASRAARVDPARALREEG"
     gene            27279..27905
                     /locus_tag="VLE22_04315"
     CDS             27279..27905
                     /locus_tag="VLE22_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSB13658.1"
                     /translation="MTVVTNGLQSRRARKKAATRSQIIAAAVRLFSEHGVEAVTVDQI
                     AAAADVGKGTIYNYFQTKEDIVVDFMVDLERSVQAQVRDFKSRQRSVAGVLTNFVQTQ
                     FRMKEHYHAFVRVFLGHMFLHTEQFLPYMAEMQKVLDPPLEALFGSLQQRGAIRSDVD
                     LRELILMFKTIQLGLTALWAIEGPPFQQVARIIEQEITLFCEGLEVAR"
     gene            27902..28786
                     /locus_tag="VLE22_04320"
     CDS             27902..28786
                     /locus_tag="VLE22_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13659.1"
                     /translation="MMIRLMISIVVLGALMGSQDSSPAEGLLSRRYRDGERLNYLMKG
                     RNNGRTYEVRLTAVVKKGTDGQFVEEYAWSDLVVDGVPQLLTPASREFRQAVTLAGGS
                     PFTMPDLSKVQPGLIGPVTDLLTFYADLFLATHAGQLREPGDRFFFANPTVGSWADGT
                     RVLIGEDSIDFDIHLTAVDRSRGVATLLVKHVPPKAPKIRIPAEWMREPVAGVPNNWV
                     QVRKAAGAYIGAVGKETFDVELTIRLSSGEILSATMDNRVEAMERECVDVELSNCGER
                     RPDPTIRHIEMSLTNSGR"
     gene            complement(28852..30042)
                     /locus_tag="VLE22_04325"
     CDS             complement(28852..30042)
                     /locus_tag="VLE22_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13660.1"
                     /translation="MTRTERKYELAVSAAVAAVFAAFWVIVFAMGDPPEFQSQYVFSA
                     AMVAAVATLWVIAVRLLWRAWWARAGASVSAMDGPALLLASGVATLPDDRRDWGAAMT
                     AELAQVRNRSSRWWFAAGCARVAIFPPRSSRMPVLMAGVVAATAVATAWPVVGYALPA
                     MRVFAVTLAALVGAMATLAAARSIRVRQAMPGPTITGTGVVGVAACIAVTAYFLVKHP
                     TAAEHLPPIGAVTFAVVLTGCLWLALTPPHGLTTSRLARGLGAGAALTLGLGFLVTSR
                     LTINTYAGPWIWVIFAPIVIFFAAAAVAAAAGRSFRAGVQAAVWTALIGTLLVYAITL
                     PEAMHRYAVDGWTLGDGELGYPVGVNLPDAIWVLVLLPVVGRPIGVLGAAAGNARWRR
                     RLAP"
     gene            complement(30039..30356)
                     /locus_tag="VLE22_04330"
     CDS             complement(30039..30356)
                     /locus_tag="VLE22_04330"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSB13661.1"
                     /translation="MPRRPSPQTTAVLFALAEDPPAWRYGYDLCRQTGLKAGSMYPIL
                     MQLADRGLLETTWENDAPLGRPPRHLYRLTGKGLELVTELPTPARAAKAKRARLRARL
                     EGA"
     gene            complement(30539..32440)
                     /locus_tag="VLE22_04335"
     CDS             complement(30539..32440)
                     /locus_tag="VLE22_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSB13662.1"
                     /translation="MNRSLPTRKLREHPDLDQLKRQAKELLEAFTTGDAAAVAEVNAH
                     YRAASPVTFALHDAQLVLARAYGFDSWPKLRAFVDGVTVKRFEEVARNGDIAQLRVML
                     KHRPELVNSAALRHAVLRRDDTMVRMLMEHGANARTGVYPHRDATTPLVIATDRGYDE
                     IVVTIKEVERRRRETNSAGLGAPDPEVLFGAIASGENDRAIGLMESEPSLIQARLLHH
                     GWTPLHVAAAKLNVQMVNWLTERGADVNAKGRNWTEQTPLDTAAHSSDANKSAEYAVV
                     ANRLVRAGAEMTPWAAVALGDAEWLRARHAQGSLTNPIEGTGGMLRIAASYNRADILQ
                     LLLKLGFDPNERTRMEDVDADSIAFTWGMPLYHCAQFGKCELAEILLKHGADPNGKVY
                     ASGDPVFQAYDKRDWKMVELLQRYGGVPEATTAGLFRQTELARKMLAGEAKYGSEGVG
                     GDTLAEQLLWGGACGGDAEIVRMALEYIDRPKDDPWWFHIMEQPIRMWSDGGAQLDCL
                     KLILERCDPNILGRSGDGFNLTILHSVAGARPHVTEEERLAFATVLLDAGGRTDLRDG
                     LLNSTALGWACRWGRAELVKLLLERGADPVEAGAESWATPRAWAETMKHDNVLQVLLK
                     YGVQEKKPN"
     gene            complement(32814..35021)
                     /locus_tag="VLE22_04340"
     CDS             complement(32814..35021)
                     /locus_tag="VLE22_04340"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HSB13663.1"
                     /translation="MTDRVSRREFLGVAGTLIAATKTQAAGDDGEPWYRQIQRCGQTN
                     LNEKDLTGLDVEEWAEYWASLKVDVVLLNGGGIVAYYPTTAPYHHRSQFLGERDVLGE
                     FIAAVRRRGMRVVARMDPNFAWEDALRDHPEWFQRDADGKPRTDPESTWLYRTCMFSP
                     YFTEQLPKIFREVSSRYDIDGFFTNGWPTTGAPVLCYCENCARVYREKTGGTPERTAP
                     SDPRYRKFFDLHMERILEIWRLWDETAKVKKRDGVFYGNLHTNLRAVKDLTKLAEAAE
                     WFNLDGQDRGGDTNPLWYCAQQVRAARSAMKGRTVTNVCGAYASTNYMAWRHTSKSPE
                     EATLWLAATVAAGAAPTYHWLGASPVDNRWRDAGREFYQWTARHSEHFRNVRPLADIA
                     MVYSQRTLAFYGVEGSRAYRDRREATDHFQGLYYALLEGRHAFDFVHENDLSPETLRQ
                     YRALILPNVANLGDRQCMQLRDYVGRGGSLLATYETSLYNEWGDRRPDFGLAEVLGVS
                     STGDAQGPLRNSYGRVEKADHEIFDGIGPTELLPGAVYRVPVRVTGKAEAPLSYMPPF
                     PSHPPEMVYPRTLRTDESALVLRETDGGGRVAYFPGDVDRTCWRTSNLDLDRLLQNAI
                     RWVRGGRTAQGPAPVEVRGDGVIEIFAWETKPGYAIHLVNYTNPHMLGGWVREFYPVG
                     RQEITLRVPEDIRIAEVRALRSGRRLPFEHRGGLVRFDVPQVVDYEVAAVVRR"
     gene            complement(35151..>36478)
                     /locus_tag="VLE22_04345"
     CDS             complement(35151..>36478)
                     /locus_tag="VLE22_04345"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HSB13664.1"
                     /translation="YEPTTSVTEVANHIGNRFGASFKAMPWKVSDVMEERFRLIERWR
                     EGGDSITELAARFGVSRKTVYKWLERFEESGLEGLKDQSRRPLVQAGRTSAEIEQWVS
                     DLRGTHPSWGPRKLRRWLERKMPEKSWPAESTMGLILKRHGLNGKRSKRRHATPSNQP
                     LAHASRANQVWSIDFKGWFRCGDGSRCDPLTVSDAATRFLLCCRATPSTDTEAVKCEL
                     KRVFRHYGLPERMRSDNGSPFASTGVGGLSALSVWWVKLGIVPERIEPGEPQQNGRHE
                     RMHRTLKAETALPPAATVAAQQKRFAEFLGIYNHERPHEALGGETPAEQYQRSAREFP
                     RKLPEVEYPAGWPLRLADEGGKIRWKQARCRVGRALAGEVVGLEEIDDNVWRVWFGPV
                     LLGLLDEREGPSRAASKIDSHWPPLQSPSGLLARRPMTEHHDDEEQEKV"
BASE COUNT         6456 a        11903 c        10769 g         7350 t
ORIGIN      
        1 acgtggacca cccggactag accgcctagc ccgaaatacg gcaggtcgcc tattgacacg
       61 gcccgccggt cgagtacaat ctgaaggtcg cctttcttta cccgacggaa gtgccatctc
      121 atgttgagaa ctgctaaacg aaaccggccc gccaccgtag gtcctccggg aacctacgcg
      181 tccagtgaag tctcgcgcct cgctcgtgtc agcctgcggc agcttcagtg gtgggacgag
      241 aaacacgtcg tctctccccg ccatcaaggg cacaagcgaa tgtacacccc ggaagaagtt
      301 ctggaaatca cggtcatcgc cgaattgcgt cgcaagggct tctccctgca gaagctccgg
      361 cgcgtcctga agtaccttca gcgggacatg ggccgccggt tgagcgacgt cctgcacaac
      421 gactccgatc tgcatcttct gaccgacggg aaggcgatct tcctggagga ccggcgggac
      481 cgcgtgatcg atgtcctgaa gaacgcccgc cagccgatgt tccttgtctg cgtgagcgat
      541 cacgcccggc gtctcggctc cgctccgaag aagaccgttc gctcggaaac gattgcggcg
      601 ggccgaaaat cccgcgcagt ctgagcccgt gccgggcgct ggccagaggc cgcgcctgct
      661 gggatataat tgaagtgctg ggagtatctc aatgacgtcg cgccgccgac acctattttt
      721 tgtgcctctg attgttgtgc tatccaccct tctgggtggt gtattcggtc ccggtctgac
      781 gggcgtttcg gcggcgagcc cgcaggaaca ggatgaaatc caggcgagcg ttcgttcgtt
      841 ctcgcgtctc tatgcggtag ttgagcagaa cttcgctgag ccgctcgatc cggacaaggc
      901 cgtttacaat ggcgccatcc cgggaatgct gcggaccctc gacccacact cgagtttctt
      961 cgaccctcgc tattttcagc tcctgcgcga ggaacagaag ggtcactact acggcgtggg
     1021 aatgaaggtc gggccccgga acaacaagac catcgtgatc gaggtcttcg ctggcgcgcc
     1081 cgcctacaag tccggcctgc gccccggaga cgtgatcata gaagtgaacg ggaaatcgac
     1141 cgagggactg aacaccactg aggttgccga cctcctaaag ggtccgcgtg gcacggccgc
     1201 cgtcgtgcgg atcatgcggg ctggaaacga gcagccgttg gtgttcaaca tcatccgtga
     1261 cgaaatctcc cgcaagagcg tgccggatgc gtactgggtt cgtccaggta tcgcctacat
     1321 gcatattcag cagttcaacg agacgaccag ccgcgaggtg gacgaaaatc tccggacact
     1381 cggcgaagag agtgtcaagg gtctgattct ggatctcagg ggcaatcccg gggggctgtt
     1441 aaacgaaggc gtggcggtag ctgaccgttt cctgcggaag gggcagacga ttgtgtctca
     1501 ccgtgggagg gcatcggccg agaagccgta cgttgctagg catggcaacg gcgggcgtga
     1561 ctacccgatt gtcgtccttg taaaccgtca atccgcctcc gccgctgaga tcgttgccgg
     1621 cgcattgcag gaccacgacc gcggctgggt tttgggagac aacacgtttg gcaagggact
     1681 cgtgcaaacc gtctaccctc tatccgagaa cacgggcctc gctttgacaa ccgccaagta
     1741 ctacacgccg agcggccggt tgatccagcg ggactactcc agcagatcgt tcttcgacta
     1801 ctactaccgg aaaaacaccg atacgaagaa ccccctggac gcgaaaatga ccgacagcgg
     1861 acggacggtg tacggaggcg gaggcatcgc accagacgag aagttcgttc cgcccaggct
     1921 caaccgcttc cagatggaac tgtacaaaga ctacgcgttc ttcaatttca cgtccaagta
     1981 cctcggcaca cgggacataa aggtgacgac ggattgggaa cccgggcagt cagtcatggc
     2041 cgagttccgg cagtttctga tcgacgagag cgtttcgttc aacgacgtcg aattcgccca
     2101 gaacgaagcg tgggtgaagt cccaactgaa gcgcgagatg tacgcttcgg ccctcagcat
     2161 cgacgcggcg aatcgcatcg cgatcgagtc ggatccggca gtggagaagg cagtccaatc
     2221 gctgccgaag gcgcgcgccc tgcttgaaac cgccaagaag ctgatcgccc agcgtagcgc
     2281 gaaatagcgt ccgtgacacg cttggatccg ctctccgctt tcaccggcgg agcgtggtct
     2341 cttctttcgt gatgaccagc tacattcccg aaccccaggt caccgttctc gatacgggtg
     2401 gccagtactg tcatctcata gcccggaaag tgcgcgagtt gggcgtgtac gccgaggttc
     2461 gcgccagcga gacgccggcg aacacgctga ggcggcgcaa ggcgatcatc atctccggcg
     2521 gtccgaacag cgtctatgag gcaggcagcc cgaccgtgga cgccgctatc ttctcgctcg
     2581 gcacgccagt gcttggcatc tgttacgggc agcaattgat ggctcacctg ctgggcgggc
     2641 aggtgcggaa gggcgacaag ggagagtatg ggctggcgct tctcgagatg ggcgctcggg
     2701 aagggctgtt tcagggtctc tccggaagcc agcagatctg gatgagtcat cgcgacacgg
     2761 tggcggaagt tccaccgggc ttccgcgtca ccggccatac cggaacctgc gccgtcgcat
     2821 cgatgtccga cggttcccgg cggctttacg gcgttcagtt tcatccggaa gtggttcata
     2881 cgtcgcgcgg cacggagatc ctctccaact ttctcttccg gatcagcgag tgcgaacgcg
     2941 actggaatcc gaaacaccgg ctgccgctga tcgaagatcg gatccgggaa gttgtcggca
     3001 accgaaacgt attcttcttc gtcagcggcg gagtggactc gacagtcgcc ttcacgctct
     3061 gcgtccgcgc tctcgggcat gagagggctc gcggaatcta cgtggacacc ggcctgatgc
     3121 gcgaaggtga gaccgaattc gtccggtcgg ttttcgagaa actggactcc ggcgcgttcg
     3181 aggtgtgcac cgcccaggag cagtttctga gcgcgctaca aggtgtccat gaccctgaac
     3241 aaaagcgcca gattatcggc gaagaattcg tcaggatcca ggaccggatc atcgacgcgc
     3301 atcatttcct ggacgaacac tggatcctgg gacagggcac gatctatccc gacaccattg
     3361 agtccggcgg gacggagaag gctgccgtca tcaagacgca ccataaccgc gtccccggaa
     3421 tccgccgcct gttggaagcg aaccgcattg tcgagccgct cagcttcttt tacaaggacg
     3481 aggtccgtga agtgggccgg gagttgggac tccccgagga gatgctcgac cgtcatccgt
     3541 ttcccggtcc cggactagcc atccgctgtc tctgttccga gacggaagcg ccagtgcgcg
     3601 aagtcgagga cggctggatc gtcccggttc gctccgttgg cgttcagggc gactcgcgga
     3661 gctacgcgcc ggtgttggcg ctccatcgct ccccctcttc gagggaagat cttcacacga
     3721 tcgcgacgga gatgatcaac cgtctgcccg gggtcaaccg gattgtcgcc tacgtcgcgg
     3781 gacacagctc gttggcccgc ttgcgagtcg ccgcgagctg gttgtcgccc gagcgactgg
     3841 cccgtctgcg ccgtgtggat gagatcgtcc ggcgtctctc ccacgacagc gggttcgaca
     3901 ggacggtgtg gcagttcccc gtaatcctga ttccagccgg cgccgacggc cgtcccgact
     3961 caatcgtcct gcggccggtg cactcggtcg acgggatgac ggcacgatcg gtcgcgatgg
     4021 atcccgtgct gttggagaag atcgccacgc gcctcctcga tgtcgccggg gtctgtgctg
     4081 tctttttcga cctcacgcat aagccccctg ccaccatcga atgggaatag cggcccgccc
     4141 gggcccattg ccgcgctgtc tcgactataa tgcggaccgg aggtcgaaat gaccgagcca
     4201 caggtgtcgc tcgagggcat gggcgaactc aagcgccttg ccggggtctt ctgggagccc
     4261 aagcccgtat ttgaagagtt agccgcccgc ccgcgctggt gggtgcccct gatcttcctc
     4321 accgtgctgg ctctcgtcta catcgggacg ttctcccgcg tgatcggctg ggacacgttc
     4381 attcgccagc agttcgaaac aaaccccaga cttcagagcc tctcttccga ccagcgcgcg
     4441 caggcgatgg agatgcagat gaagatcggc tccaggttcg cctacgtcgg cggcgcgctc
     4501 ggcatgacgg tctcgagtct cgtaatcgcc ggaatcctgt tcggtgcgtt caaccttctt
     4561 ggtggcgcca gcctgaagtt caaacaggcg ttcagtatta cgtgttactc gttactgcct
     4621 acagcgctat cgtccatcct cgccttgatc gtcatgctct tcaagaatcc cgaggatttc
     4681 gaccttcaaa acccgcttgt gctgaatgtt ggcgctttcc tcgatccggc ggcgacccca
     4741 aaggctctgc acagcctggc gacttcgctc gatctgttca gcatctgggt gattcttctg
     4801 ctggcgctcg gcttcagcgt cgcggcccgg cgcatctcat acgccaagtc actgatcctc
     4861 gtcctggtgc cctgggcgct ctacgtcgca ggtaagagcg gctgggcggc tatgttcgga
     4921 tagattcgtc gggaatccgg tcagatccgg tggaacccgc gtagaatatc gcgggtggag
     4981 taacgtagcg cgatcggacg tccgtgcggg caactcatcg ggcactccgt ggctgccaga
     5041 gctgacagca gccattccat tttgctctga tccagccgca tgttcatctt gattgccgcc
     5101 cggcatgcga tcgaggcggc cattgcccgc ctcaggtcat ccatcgaaac accccggagt
     5161 tccttctctg ccgtctcgag gatctcgaac acgactctct ccacgtccgc ttgggccacg
     5221 cccgcgggcg acgccttgac ggcgatcgtg cgatggccga aaggctccgt ctcgaagccg
     5281 acgtgatgaa gttcgtccgc gatgcgtgcg tactcgatct gttgtgccgg cgtgagttgc
     5341 agtatgagcg gcagaagcag ccgctgcatc tcgacgcgcc cggaggcgct ttgcctcagc
     5401 accttctcga acagaatccg ctcatgagcg acgtgctggt cgatgatcca gagcccgtca
     5461 cggcccgccg cgatgatgaa gctgtcatgg atctgcccaa ggggccgcag atcagctagc
     5521 acagcgaggc ttccggatga gtccgccatg gcctctgctg ggaatcctcc gtgggtgtcg
     5581 ggaatgcgca cccgcatcgg ggatacatcg ggctcgggta cgccgggctc cctcacggga
     5641 aacggggctc cgccgaagtc gaagcgagcc tctggaggct gcggagcccg cagtgtgaca
     5701 tccggcagaa actccggcgc ttcgggcacg gcgcccgaca cgacctgcga cggaggctcg
     5761 ctccaaacct ggttctcgat acgctgcgtg aactccgaat acggcagccc tgccgcggcc
     5821 tgccctcccg gaagatccgg cgaatgctgg ggcagcggaa gggaggagat cggccgggac
     5881 tccatcagag tctcgcggat cgaatcccgc acgaagtcgt gtacaaacga gccgtggcga
     5941 aatcgcactt ccgtctttga agggtgcacg ttcacgtcga cctcctcgaa gtcgcactca
     6001 aggaatagca atgcgaacgg atagcaagcc ggaggtatca ggttgtgata ggccgaggag
     6061 atggagtgca tcaagaggcg gtcgcggatc aacctgccgt tcacgaacag gaagatcgag
     6121 ttgcgattca gcttttgaac ctgcggacgc gagacgaagc cgctcagacg gaacgtgcgt
     6181 ccgggcggtt cctccgtttc gtctgccgga gcgcccggcg gcacaaatgg agccggaatc
     6241 gccaggtggc gctcgcgaat gctgagatct accagttcgt ccagtgtctg gctgccgaac
     6301 acctggtaga cccgctcttt caaggtggga acgggagtga cgtagagcag gtcgtcaccg
     6361 ccgcttcgca gcaggaaggt cttgccggga tgagccagcg aatagtgcgt aaccagggac
     6421 gcaatgtgcg ccagttcggt ctgctccgac cggaggaact tcttgcgcgc gggcacgttg
     6481 tagaagagat cccgcgcggt aatggccgtc ccgccggcaa gagccgcttc ttcgacgcgg
     6541 agcatctttc cgccggcaat ctcgatccgc gtaccggtgg tttcctcggc cgagcgcgtc
     6601 tccagtacta ggcgcgagac cgaagcgatc gacggcaggg cttcgccgcg gaacccgagc
     6661 gtggcgatcg aaagcagatc cttcacgtcg ctcagcttgc tggtggcgtg gcgctcgaag
     6721 gcaagcatcg cgtcgtcgcg cagcatgccg gagccgtcat cggaaacgcg gatgagccgg
     6781 cggccgccgc tgtctacctc gatcctgact tcggtggagc cagcatcgag cgagttttcg
     6841 aggagttcct taacgacgga tgcgggacgc tcgaccactt cgccggcggc gatcttgttg
     6901 gcgacttggt cggagagaac ccggatccgt cccatgcagg ttgtgtggcg cgaaaggcag
     6961 tatccccgtg ctttacagcc cgtctgccgc tgccagcctg cctattgtat cctgaccccg
     7021 cgctcctcca gcagggaacc gttgacgcgg aggaggttga gcaggtttcg ccggtagttg
     7081 atcgactccg taaggagacg ggactcggcg accgtaagcc ggttctgcgc gtcgagcacg
     7141 aagaacagca cggtggtgcc cagatcgtac tttttctgct cggcctcgta gttcttctgg
     7201 gccaggtcgc gcgccacaac cgccagccgc acggcgtctt tactcgattc cacttgactg
     7261 attgcgttga gaacttcaag ccggatgctc tgctccgcgg tgcgggcgcg caacgagttc
     7321 agcctcttct gaacaaccgc atcggcaaaa tcggcggcgg ccgcgcggtc acgcaaggga
     7381 aggcgaagag tcagtccaaa cgcatacgtc gggaagttaa agccaaataa ctggtccagc
     7441 gcgtctccaa atccgccggg gatcatcgtg gccgcggtcg tggtgtctcc caggctgatc
     7501 ggccgctgaa agaaatttcc tccgcggccg gcgctggcgt agctcccggt gagtgacagg
     7561 tccggacgca ggttgtttgt cgccgagcgg atgctgaggt cgtctgtttc gaggttttgg
     7621 agcacgctcc gcagatccgg acgcatctgg agcgccttct ccacaatcgc ttcccggtcg
     7681 aacgagaccg tatccgccgg cggcaatacg gtttcagtga gttcaagagg cacattcctg
     7741 acgtgggggt ccaggtctgc accgatctgt ttgcgaagcg cgtcttcggt ctgcgcaagg
     7801 cggaaacgcg cttgtgacac ctgaatctcc gcggcggcat aatcggcctg cggctggaaa
     7861 atgtccagcg gcggcaaggc gcccaaacgc agttcatcct gcgctcgctt tagggcgacg
     7921 ccacgcaacg ccagcgcctg ctcctggacc ttcaggttct ccctggcctc caccacgtcc
     7981 cagtacgcgc tctcggccgt ggttaacaga cggagcaact gatccctcat cccgtactcg
     8041 cttgctcgca gccggcttct ggcaataaga accggcagcc gggttatgta cgttccccgg
     8101 tttcgaagca gtggttgcgc aaacccgaag gtgagacgcg tggtaagcga gggattgaac
     8161 gtcgcgaagc tgctattcgt tgaaagtttg ctggcattga agccgacgtt gtacgtcgtg
     8221 ccgctttcca gcgtctgttg gtagttgaag ttggcgggct ggctcaactg gctcaccgtc
     8281 gccgcaccgg cgagcacgtc gctggccgga ctgttcgccc gagtggcgtt gaacgaggcc
     8341 tggagtgtcg ggtcgaatcg gccaaaagcc ctggtgatgg cgttcctttg cacttcaacg
     8401 gtcactttct gtatcgcgac atcggtattg tttgccagaa ccaactcgag atatgcacgc
     8461 agcgagagtt cgagcttatc gccgaccgcg aaatcttcga gccgtgtggg aggctgcaat
     8521 tcgactcgtg ggatcggcgt ggagaaggtt tgtcggaaat aagccggcga ggggaactga
     8581 ggcacttcct gagagtacaa gtaacctgcg cacagcagcc ctgccaggaa cgtaagaatt
     8641 cgcatgatga tcttgcttac tcgtaacgga tcgcttcgat aggatccagt tttgcggcct
     8701 gcacggccgg ataaataccg aacaacagcc cgataaacac cgatactccg aacgcgacgg
     8761 cgaccgagcc ggcggtcacg accgtcgacc agcccgccgc ggcggcgatg acccgcgaaa
     8821 gcgagaatcc aaacgcaatg ccgatcaacc cgccgacaat cgaaatcatg actgcctcgg
     8881 tgagaaactg ccgcacgatg tcggcttggc gcgcgccgat cgcccggcga atgccgatct
     8941 cccgtgtacg ctcgagaaca gtggcgagca tgatattcat gatgccgatg ccacccacca
     9001 atagagagat gccggcgata cagatcatga ctacttcgaa aatgaattgc gtccgccgcc
     9061 gttcctccag caggccggcc ggcacgacca cggtgaaatc gcccgcatcc ttgtgggtgg
     9121 cggacaaaat ggcgctgatg acactcgcga cctcgatcga gtccgtttgc ggagtgacac
     9181 gaacgtaaat cccgtcaatc tcgtccttca agtaactgtt gccatcctcg aagcgccgca
     9241 tcacggtatt caacggcgca attgcaaggt tattgcggtt tacgacttcc aaaccctcga
     9301 cttcggtatc ggcggtcgct tggtgtgcca gaacgccaat cacctgcaac cagacttcgt
     9361 tgatcttgac gtacttgcct accgccttgt cgtagccgag aaggttgacc ttggccgact
     9421 ctcccaggac gcataccggt gcggagcgaa gatcgtcttc tgcggtgaaa aaccgtcctt
     9481 cgatcacctt cagactgtta atctctagga aattcggcaa cacgccgatc agcaccggcg
     9541 gctcgtttac cgtcttgggc aggaccttcc cgggcttgaa acgcttgcgt ggggtcgccg
     9601 cctcgattcc ctgcacattc tgcgagatgg cgcgaaagtc gcggaacgta agtccggccg
     9661 aggttgcgcg gcgcgtctgc aactcgttgc gatcgacagc ttccttggct tcgatgataa
     9721 tgttgttcac gccgagcaga tcgatgaagc tgatcatctc cttctgcgcg ccggccgtaa
     9781 tggagagcat cgctaccacc gcgcccacgc cgaagatcat tcccaacatc gtcagcaggc
     9841 tgcgcagctt gtgcaccagg aggcttgaaa gccccatgta tatttccggg aggtaattgc
     9901 ccagaccgct catctcgtct caaccctttt gctcgacatc cggatcaact ctttcccgcc
     9961 ccggtcgcac cgccggtctg tcccggtctg ctctcaggct gcccggactt cttatcaccc
    10021 ttcttggcgt taggatcagc cagcgcaacg tgctcgcccg gcttgagtcc cgaggaaacc
    10081 accatcgtgg cttcgctgcg gcgaagcagc gtcacggacc gctcttcgaa ccggcggccg
    10141 gactgaacgt agacgatcgg ttttccgtcc ttctcgaaga ccgcctgtgc cggaatgtag
    10201 atcgcgttcg gaatcttctc gatcgtgatc tcgacatcgg cgagcaggcc gggacgtaag
    10261 agcacctcga gctggctgtc ctcttccggc ggcggcggaa gttgcgcatc ggcgagttct
    10321 ttctccgtga agccggaagg cggtccgccg acgaacggca ttccggggcc aattcctgag
    10381 gacgaggggc cgccggccgt ctgcgccccg gcctgtccaa ggccctcgcg tcgctgtccg
    10441 gatgcgcctg gcatcgattg gcgcaccttc gcgaagatct tctgccgttc ttcctggctg
    10501 agatcctcca tcttccggtc accgagttcc ttctggagag cctcccgcat cttctggcgg
    10561 tcggcgtcgg acatctgact gcggcgtcct tggccttcac ctcgactgcg gcgctccccg
    10621 gtggctccct ggccttcacc tccagggccg ccggagggac cacccgaacc tatccctgcc
    10681 agcaacatgc caggctcccc gccgctctcc tgtcccgggc cgcccgatcc acgtctgccg
    10741 ccgggggacc cgcccggaaa tccacccatg gcaaacgccg ggcgcgatgc cgccggcggc
    10801 ttgttccggt tcctttccgc cgtagccaat acacgctgga tctgctcggg cgtcgcgccc
    10861 accgtcgtga gtaactgctt catgtcgacg gagaagagca cgtcgaactt cttggaggga
    10921 tcgcccgacc aaatgttggc gcttgcggtc ccactcatgc tcttgatctg tccccgaaga
    10981 cgagtgccgg cgagggcgtc gggttgcatg gaaaccgctt ggccttcccg aaggttggcc
    11041 ctgtcgatct ctccgatttt agcgatgact tccatttcgg aaagatcgag tacgtccgcc
    11101 actggcatgc cgggctggac ttggtcgcct tcgcggatgt ccggcacctg catgccgaac
    11161 atccgcccgc cgccgaaccg gttctggtga atgaacacca acccgctaat cggagagaga
    11221 agacgcgtct ggctgagtcg cgacttctca cgagtcagct cgatgactgc cttgttgcgc
    11281 tgctcacgca acacggcaat ttcggccttc gcctgctcca gccgcgactt gatgtcgctc
    11341 tcgagctgct tgagtctgcg ctgcgcttct tccagattga gctcattctt gcgggcatcg
    11401 attttcgaga gaagttcgtt gcggctcttc tctagctcgg cccggcgcac cgagtaccgc
    11461 gcccgaagta actcaacctg atcctggttg ctgcggatcg ccagctctgc ctccgccttc
    11521 ttgatctgtt catcgacttg ctccagctca agttgcttct cctcaagccg ggcgcggact
    11581 tcggaatcgt cgaactcgac gatcaggtcc ttttcccgcg ccatcgcgcc gagcggagcc
    11641 agccgggtga cctgtactgt gccgaaaagg ttgggcgcgg tgagtgtcac cgaacgcacg
    11701 gcacgcagct cgccgcgcga aaacgcgcgg atcatcacgt ctccctgccg taccctcgct
    11761 gtgggaatct gttgttgccg ctgcggcaac tgccgaacga agtggtaccc gccccatgca
    11821 gccgccagca cagcaatcag gatcaacgac cggaacatca gctttttcat tacgttctgt
    11881 cgcccgctac cctcagcgtt ttttcgcttt cttcgccgcc tccgccgggt cctcgagtgc
    11941 gaccatctcg ccctctttca accctgcgag caccacgatg tcgttgtcgt ttcgcttgcc
    12001 gacctggatc acccggaccg ggaacctctg cccgcgctgc acatagaccg acggcttgcc
    12061 gtcatgcaca aaactggcgc gggccgggat cagcaacacg ttcggttcgc tctcgatgat
    12121 gatgtctacc gaagcgctca tgccgggacg cagccgtggg tccaggtttt tgagcgtcgc
    12181 gaacgtcgga aagaccttgt cggcattcat gaagcccttc cagacgagcg aagcgatggg
    12241 gctgatccag tcgatctcgg cggtgaactc cttgtccggg atggcatcaa ccctgatttt
    12301 taccaactgc cccagccgga tcttgccgcg atcgacctca tcgagtttca attcggcgcg
    12361 catctcggaa aggtcgggaa tctcggcaat ggccgccccg gtccaggcgc ggtcgccctc
    12421 cttgaatggt ggcggcatct ggccgaacgt gcccccggag cggaagttcg gaagaatgtt
    12481 aataatgccg tcgttcgggg ctagaatgac catcttttgg aggtagccct tcgctcgcgt
    12541 catgtcccgg atggtcttgt ctttcgtctc gcccagacgc gccaaatcgg cctcttgaga
    12601 cactttgtgg gagttgattg tcgttttgac ctgcccgagt tctccgcccg cgatcccgac
    12661 gttaatgcgg ttcttcgccc cttcgatctc tgagaggatt tccgccttgc tcgcctccag
    12721 cttcgcccgt tccaggttgt actcggactg catgagattc atgccgtcca tctcgttggt
    12781 catccggtgc tgcgcctgcg tctgaacaat ctggctgtcc accgtgcgca cggttgtcgc
    12841 gcgctccagg tagtactgtt cctgagtggc cgtgtcgaat tcgacgacga cgtcgccctt
    12901 cttgaccggt ttacccgact cggcgagttt cacgatctgg gggttgggta cctgaggcgc
    12961 cgtcagcgtg agtgatctgg tgctcttcac ttcgccccgc gtccgcacag cgagtacaaa
    13021 ctcgccgcgc cgcaccttgg cgaccggcac ctccacgacc tccggggacg tgtacttgta
    13081 cgcagcgtag cctgctccgc cgaaccccac cagggacaag gtccacagga tgatcttttt
    13141 cttgcgtgac gccggcgggc gcccgattct ctgctttggc tttaggtctt tggacattcg
    13201 ccgaataccg agatctctat accttctttg gcgtcgatgg aggccgctct aacgcaacca
    13261 cctcgcctgc gcggagtccg tcgcgcactg ccaccgtggt gtggcttgcc cgaccaagct
    13321 ccacctcgcg ccgctgccac ccggacttgg tctgcacaaa cacgtaaggc ttctctccgc
    13381 cgcctgtctg aaaaaccgac tcgcgcggcg caatgatggc atccgcctcc gaattgacca
    13441 cgacatcaac acttaccgac agatcgggga tgactctcgg atccatgcgt tcgagccgca
    13501 gggtcaccgg tatctcttta acgaagtccg cccgaaaccc gctggctctt gtgatggcgc
    13561 cgattgagta cacgcgcgcc gggagttcga ggtcggggta tgcgtcgaac cgtacccgtg
    13621 cgtgagctcc gatccggagc atttccgcat ccacctggtt cacgctggcg ctgaccacca
    13681 ttgagtccgg atcgacgatt cgcatgaatg gctggccggg atggacttgg tcgcccagct
    13741 ggatttgacc catttcgccg ccgcgccagg ttgtttgcat cactaccaga ccgtcaatgg
    13801 gcgccttcat tagcatgcgg tcgacgtctc gttcgacccg ctgggtttct accttagcct
    13861 ccgccaggct aagctctgcc acccgcaact gggcccgcag ggacgcctcc aggtacttcg
    13921 cttccttcaa aatctgctcg taactggctt cggcctcctc cgcagcaagc ctcagggttt
    13981 ccgtttggat ggtcgagcgg accggtatcg tcttgagatc cagacgggct ttgtcgagtt
    14041 ccgacttggc cacacgaatc gaatgttcgt gagccttccg cgcgacttcg agttcggctt
    14101 ggaaccgctt catgctagcc tcggcttgcc gaacacctgc ctcgaaatcg tcgagccggt
    14161 tctccatgtc ctggcgatcg aaaccagcca cgacgtcacc cttcttcact cgggctccac
    14221 tggcggccag ttgctccaga atcagccctc tgccgaatcc gccgccaccc ccgccgccgc
    14281 cgccaccacc gacaacggcg ttgctcgtgg acccaagccc cgaagatccg agagcgctgg
    14341 agctgctgct cgacgaagtc gaacgcgacg aactgctgct cgaagaggag gtctggctgc
    14401 cgaatcgggt tgtcgccgcg cgaaaggccg aagcagtgga tctccccgct ccggttcccc
    14461 cgctggcctg agacattgca ctcccactcg acccggtgcc cccggccgaa gccgtggacg
    14521 tagacgacga atcggattcg ccgcccgcgg aggcaagcga cccggatgcg gatgatgaag
    14581 aggaactatt gacgctgcgc gtcgccgccg tgctcactga cgggatgcgg acgtcagagt
    14641 ttcccccatg ccctccgcgc gtccggggcg cattgagcgc ggcgtagttc agcgccgtgg
    14701 ttgtccctgt caaccgcacc gtttgttcga gagtgcctgt cgcgactcgc gccgtgcgca
    14761 ccatctgggc gacggctggc ctgcttgcct gcctggacca gcgcgctcca aggaatagaa
    14821 gaagcgccgc aaggctggct aacactaaga aataaggcca cttccgccgc ggaggtttcg
    14881 cttgccggcg gttttcccgc gccggcgcgc caggcgtggt tgtcagaacc gggttttcgc
    14941 tggcttgggg ctgcatcgaa ggcctacacc attcctattg tgcccaattc gactggacca
    15001 aatgaaaaga cgctgctgtc ggccctgagg ttatgggtct gttgcggaaa gtgaagcaaa
    15061 ggaaagctcg aactgctcgc gggccgcagc cgtccccgga caggccgagg cacaggagtc
    15121 cggcgaacct cttgttaacc gctcatcgcc gctagaaact cggcgttgct ccgcgtcttt
    15181 ccaagcttgt cgagcagcaa ttccatgcac tccaccggcg acagcgggct caacaccttg
    15241 cgcaacaccc acacacggtt caattcttcc ttcggctgaa gaagctcttc cttcctcgta
    15301 ccgctcttgt tgatgtcgat cgccgggaag atacgcttgt cgaccagttt tcggtcgagg
    15361 tggatctcca tgttgccggt gcccttgaac tcttcgaaga ttacgtcgtc catgcggctg
    15421 ccggtgtcga tcagcgacgt ggcgataatg gtcagcgatc caccttcttc gatgttccgt
    15481 gccgcgccga agaaccgctt cggccgctgc aatgcgttgg agtccacgcc gccggagagc
    15541 accttgccgg agggcggcac gacggtgttg taggcgcgcg caaggcgggt aatggagtcg
    15601 agcagaatta ccacgtcccg cttgtgctcg accagccgct tggctttttc aatcaccatc
    15661 tcggccacct gcacgtgccg cgtggccggc tcatcgaatg tcgagctgat cacctcgccc
    15721 ttcaccgagc gctgcatgtc cgtcacttcc tcgggccgtt cgtcgatcag cagcacgatc
    15781 agggcgacct cgggatggtt gacggccacg gaattcgcga tgctctggag cagcatcgtt
    15841 ttgcccgtgc gcggcgggga cacgatcaaa ccgcgctgtc ctttgccgac cggcgtgaga
    15901 aggtccatca cgcgcccgga gaaattgtcc ttcaccgtct ccatccgcag ccgctcgttc
    15961 ggatagagcg gagtcaggtt gtcaaaaaag atcttgttgc gcgcctcttc gggcggttcg
    16021 aagttgatcg cgtcaacttt gatgagggcg aagtagcgct ccccttcctt gggcggccgg
    16081 atctgccccg agatcgtgtc gccggtgcgc aagtcgaaac gccggatctg ggacggcgag
    16141 acgtacacat cgtccggacc gggcaggtag ttgtactcgg gcgcccggag gaagccgaag
    16201 ccatcgggca ggcactcaag cacgccctcc gagaagatga gcccgctctt ctcggcctga
    16261 gcttgaagaa tcttgaagat cagttcctgc ttccgtagac cggccgcccc ctgaacaccg
    16321 aagtccttgg cgatctggtt gagcttctga atgctcatct ctttcaactc gaccagatcg
    16381 agggtcgtgg agccgttctt tgcctgctgc ccgggaccat ggcctccgtg cccttgtttt
    16441 ttcctcgttg gcggagtcgg aggggtagta gtcggcggag gaggaggcgc ggcagccggt
    16501 tgttcgggcg cctttgcctc gtcagcggca acggtctctc ggttctcttg gttttccagc
    16561 tcgtttgcca atttgcgtca ctccttggcc gattccggcc cgttgcggta gcgggaagcg
    16621 gaacgttctg tcgttccagg gaggctcacg gcgggatcgg gccgtacctt gtactacctc
    16681 gtactacgtc gattgcactc cccgaacaat gcacctctca gtgcagcttt tcgtcgtgct
    16741 gagaagcgat ttccacggtc gaagggccgg gggctatcgg caatgcttca atctcccttt
    16801 ccagggcgac tttcagcact tcgtccattg actcgacgaa gtgcaacctc atgtcctttc
    16861 gaatgacttc cgggatgtct ggcagatcct tctcgttgtc cttcggcagg atcacttcaa
    16921 agataccatg ccggtgcgcg gcgaggagct tctccttcaa ccctccgatc gggaacacgc
    16981 gcccgcgcag ggtgatctcc ccggtcatcg ccacgtcgca acgaaccggc accttcgcca
    17041 gggcgctgac gatcgtggtg gcgatcgtga tgcccgctga tggcccatcc ttggggatcg
    17101 ccccctctgg cacatgaaca tggatgtcca ggtggcggta gaaatccttg ggcaacccga
    17161 tcgaatgtgc gcgcgagcga atgtagctca tcgcggcttg ggccgactcc tgcatcacgt
    17221 ctccgagctt tcccgtcgtg gtcagcttgc cccggccctc catgatcgtc gcctcggtgg
    17281 tcaggatctg cccgccgacc tcggtccagg ccaggcccgt cgccgcgccg acttcattct
    17341 tcttatcggc ctccaggtcc ctaaacttca aaggcccgag aaacccagga atcgtttctt
    17401 tggtgatcgt aactagcgga aactcggctt tcgcgtcggc cgtctggctg gtcacaagcg
    17461 accgcgcgac cttgcggcac aggttgccga tctcccgctc caagttccgc acccccgctt
    17521 cccgggtgta gctccgcacc agcgtcagca gaccctcatc cgtgaagcgg atctggttgt
    17581 ccccgagccc gccctgctcc aactgcttct tcaccaggaa ccgcttggcg atctccagct
    17641 tctcaagctc cgtgtaaccg gaaagccgga tcacttccat ccggtcctgc aacgccggcg
    17701 gaacggtgtg gagcacgttc gcggtggcga tgaaaaagac ctggctgaga tcgtactcta
    17761 cgtccaggta gtgatccacg aacataaagt tctgttccgg atcgagcacc tccagcagcg
    17821 ccgccgatgg gtccccgcga aaatccatcg acatcttgtc cacttcatcg agcatgaaga
    17881 ccgggttccg ggtgcccgcc ttcttcatca tctggatgat ctgaccaggc agagcgccga
    17941 tgtacgtccg tcggtggcca cggatctcag cctcgtcacg cacgccgccg agcgacatgc
    18001 gcacgaactt gcggcccgtc gcgcgggcga tcgacattcc aagcgacgtc ttcccaaccc
    18061 cgggaggccc gacaaagcag agaatcgaac ccttcgggtg ctttaccagc cgacgcaccg
    18121 ccagaaactc gaggatgcgc tccttgatct tttcgagacc gtaatggtcg gattcgagca
    18181 ccttctccgc aaacttcaaa tcgcggatct ctttcgtctt cttcttccac ggcaccgcca
    18241 gcagccagtc gaggtagttg cgggagaccg tagactcagc cgacatcggc ggcatgttct
    18301 ccagccgccg caattccgcc atcgccttct cgtgggcgtc cttggtcatg ccggccgatt
    18361 cgatcttctt cttcagctcg tcgaactcgc ttttttcgcc ccggcccagt tccttctgga
    18421 tcgccttgat cttctcgttg aggtagtact ccttctgcgc gcgctccatc tgccgcttca
    18481 cgcggccctg gatcgtccgg tcgacattga gtttctcgat ctcgatgtcg agcatctccg
    18541 ccacccgcgt cagccgctcc acgggatcga agatctcaag cagttcctgc ttttcctcga
    18601 tggtgagctg gaggttggcg ccgacggtgt ccgccagccg tcccgggtcg tccacccgaa
    18661 tcgccgcgat catcgtctcg tagttcagat tctggctgag cttgacgtac tgctcgaaca
    18721 gcgtcgtcac gcggcccgtc aaagcgtcga tctgcggacc tgcctccact ttgaagccga
    18781 acgtcttcac caccgcgcgg aagtagcctt cgtcttcgcc cacggaaacg atcttggcgc
    18841 gctccacccc ctcgaccagc actttgatgt tgccgtcggg cagcttcagg ctttgcacga
    18901 tattgacgat cgtcccgacc gaataaatct cgtcgggacg cggctcatcc acggaggcgt
    18961 cgtgttgcgt cgccaggaag attttcttct cgccggccag cgcctcttca agcgcccgca
    19021 cgctcgactc gcgtcccacc acgaacggcg tcatcatgtg cggaaagatg accacgtccc
    19081 ggacgggcat catcggcaat cgcttcgttt ccgaacgttc cttcgatgtc aacattggat
    19141 tatgctgtcg ggcggcgcgc agccctgatt ttgcttgacg ggaaaacttc cagatacaac
    19201 tcccaggttg gcgccggatc ccctgtcgct tattctaccc gctgccggtc cggagaggct
    19261 atccagcctt ctctaatacg gctaaattta tcttctgcgt catgaccatc tccttcgtca
    19321 ccagaaattc ccgcactttc ttgtatgaag ggagatagta catgaggtcc agcatcagat
    19381 cttcgagaat catgcggagg cctcgggccc ccacttttcg ctcgagcgcc aggctcgcga
    19441 tcgcctccag tgcgtcatcg ctcaatttca gtctaacatt ttcgaactcg aagagccgct
    19501 gatactggcg cacgatggcg ttcttcggct tcgtgaggat ctgaaccagc gcgtccttgt
    19561 cgaggtcctc gagcaccgaa accaccggca ggcggccgac gaactcgggg atgaacccga
    19621 agcggatcag atcgacgggc tgaatctctt gcaggaggtt cgtgtctcgc cgcgaatttc
    19681 cgcggcgagc gccctccggc gcatcttccg ccgtcatgaa gcccatcgcc ttccggccca
    19741 ctctccggcc aatcaccttc tccagcccca caaacgcgcc gccgcagata aacaggatgt
    19801 tcgacgtttc aaccggcgtg aattcctggt gcgggtgctt gcgccctccc tgcggaggaa
    19861 cgttcgcaat cgttccctcc agaatcttca gcagcgcctg ttgcacgcct tcacccgaga
    19921 cgtcccgggt aatcgacggg ttctcgtcct tgcggccgat cttgtcgatc tcatcgatgt
    19981 agatgatgcc ctgctggcag cgctgcacgt cccaatcgga gttctggagc agccgcagaa
    20041 tgatgttctc tacgtcttcc ccaacgtaac cggcttccgt cagcgtggtc gcatccacaa
    20101 tcgcaaacgg cacgtcgaga atccgcgcca gcgtctgcgc cagcagcgtc ttgcccgtgc
    20161 cggtgggacc gatcagcagg atgttggact tggaaagctc cacatccgtg gtccggtgct
    20221 tgttcatctg gacgcgcttg tagtggttgt atacggccac tgcaagcttc tttttcgacg
    20281 cctcctgccc gatcacgtac tggtcaaggt actccttcag ttcgaggggc ttcggtagct
    20341 tatgggcact ggctcggggc tgctcattcc ggtcgtcctc caggatcgag ttgcacaccg
    20401 cgatgcattc gtcgcagatg tacgctcggg ggtaatcgct gggggaggat atcagcttcc
    20461 cgacgacatc ctggctcttg tgacagaagg aacaccggag cgagtcatcc gaaccggtcc
    20521 gcttcacaaa gtgtctccca ggatcggata gtcttggctc ataggcatcg caagaaatcc
    20581 acccccatta tcacctacac cgggcgggca atcaatgatt ctcttaatgt gacctgccat
    20641 agtcccagtc cgccgaaagt actagtcctc tgccaccttc cgggcgtgct cgaacaaaaa
    20701 ggtcagcgtc ttctccgttc ggatgcgcga agcgatccgc cgcaagaccc cctgctcctc
    20761 cagacgccgc cgcaccgccg ccaccggctc ccggtcctgc cgcgcgatcc gctgcacttc
    20821 gcggtccact tcgtcctgca tgacttcaat cgcctcacgg tccgcgatcc gctcgagtag
    20881 tagcgacgcc ttgacgtctt tcgtcgcctg ctcccgctgc gactcccgca ctttctgcca
    20941 gtccagcttc accgacctcg gatctcctcc gctggcggcc acggcccgga gatactgttc
    21001 cacctggttc tcgatctgcc ggtccagaaa tgcctccgga accggaaact cgtgcatctc
    21061 aaccagcttc tcgaccagct tgttctttgc ttcctgctgc gccaggtact ccttctcgcg
    21121 cagcagagat ttgcggactt ccgaccgcag ttcgtccatc gtcaggtagt caccgagatc
    21181 cttggcgaac tcgtcgttga tctccggcaa ttctttccgc cggatcccct tcatcgcggc
    21241 gcggaaccgc accgacttcc cggcgagctt cgcgtccccg aagtcttccg gataacgtac
    21301 atcgaactcc ttctcgtctc cgggcgacat cccgcgaaga ttctcggtga acgcgtcgag
    21361 cgtatcctgg cccccgacgt gaagcatcac ctcgtcctgc ttgagcggct tttccaggcc
    21421 actcagcgct tcgagcgcca ccaccgcatg gtcgccgtcc tccgccgggc gtgggtccac
    21481 gttgacgtag tctgccttct gctctcgcag ttcctcgatc cgccgggcaa cgtcgtcgtc
    21541 ggagacctcg ggatcctgat acgccaccgt cagaccgcgg tagtcctgaa gctcgaactc
    21601 gggactcacc tcgaactcgg ccttaaaacg aagaggttcg ccggaatgaa agtggacatc
    21661 ggtgatgtcg ggggtcccca caacacgaag attcctgtcc tcaacctctt tctggaagcg
    21721 cttcggcacc aggtttcgga tgacctcttc gcgaatgtcg gacgaaaaac gcgtgcggat
    21781 gattcccacc ggcactttgc cggggcggaa cccaggcagc cggacgcgct gggaaaggga
    21841 ctcgaccaca cggttcgtct ccttctcaac gtcctcgatg ggaatcgtga tttcgagtgt
    21901 ctgcttgcag ctttcttgag aggccatttt ctatgacggc gcgaaatacc ggacccccgg
    21961 gggagccgcc cgaagcggcg tccccggagt ccggaaaatc tcggactacg taccttcgga
    22021 ccaggaggcc aggtacttct cttgctcggg cgtgagccgg tccactttga cgcccattgc
    22081 gtcgagtttc attcgggcga cttcccggtc gatcgtctct ggcacggagt acacacgctt
    22141 ctcgagcgac ttgtgctcgc gcaccatgaa ctcggcggac agcgcctgat tcgcgaagct
    22201 catgtccatg acggaggcgg gatggccttc ggcggcggcc aggtttacca gccgtccctc
    22261 gccgaggacg tagatgcggc ggccgtcccg catcacgaat tcttccacga attcccgcgt
    22321 cggccgtcgg gaggacgcca tgcgccccag cgcctccaga tcgatctcca cgttgaagtg
    22381 ccccgagttg gccagaatcg ccccgttctt cagcttgtcg aaatgctcgc ggcccatcac
    22441 gctcttgttg cccgtgacgg tgacgaacac gtcgccggtc tcggcggcgt cgctcatcga
    22501 catcacccgg tagccgtcca tcagcgcctc gagcgccttg atcgggtcca cctcgcagac
    22561 aatcacgttc gcgcccattc cgcgggcccg catcgccacc cctcggccgc accacccgta
    22621 gcctgccacc acaaccttca ggccggccat caggacgttt gtggcccgga tgatgccgtc
    22681 gatggtgctc tgccccgtcc cgtaacggtt gtcgaacaag tgtttcgtgt gggcgtcatt
    22741 gacggccacg atcgggtatc ggagaactcc ctcgttggcc atcgcccgca ggcggatcac
    22801 tcccgtggtg gtttcctcgg tcccgccgat caccacgtcc agcagttctt tccgcttggt
    22861 atgcagtatg gtcaccaggt cggcgccgtc atccatcgtg atctgcggct tgtgatcgag
    22921 caccgacgcg atgtgctggt aatagacgtc gttgctctcg cctttgatcg cgaaaaccgg
    22981 gatgttgtag tcccgcacca gggtcgccgc gacgtcgtcc tgcgttgaca gcgggttgga
    23041 tgcgcacagc gagaccgaag cgccgccatc ccgcagcgtg accatcaggt ttgccgtctc
    23101 ggttgtgacg tgaaggcacg ccgcgatgcg gatgcctgcg aggggctgcg tcttgatgaa
    23161 ctgcttgcgg atcgtttgaa gcaccggcat cgactggaag gcccactcgg tgcggcgctt
    23221 ccccaattcg gccagagaca gatcgcggac gtcacagccc gtggtagttt gcgtattcgt
    23281 tgacatgaac tcctcgtttg ggaggatgga ccgcgtcgta ttctgccccg cggaagcctc
    23341 cgccgcttca gaaaactata gcccgcttac ccgcgggcca atgccactcc gatgtcgctg
    23401 cgcagcgctt ccgccttgtc ggcggcctcc caactgaacg tctcttcggt ccggccgaaa
    23461 tgaccgaacg ccgccgtctt tcggtagatc gggcgccgca gtcgcaggtg ctcgatcatg
    23521 ccacggggcg tcagcgggaa gtttgcgcgc accagttcca ccagcctgtc ttcactggcg
    23581 gcgccggtgc cgaacgtatt caccatcacc gaaaccgggt ccgcgacgcc gatcgcgtac
    23641 gccagttgca cctcgcagcg ctttgcaagg ccggccgcca caatgttctt cgcgatgtat
    23701 cgcgccatgt agcacgcgga gcgatccacc ttcgtcgggt ccttccccga gaagcagccg
    23761 ccgccgtgcc gccccatgcc gccgtacgtg tcgacgatga tctttctgcc ggtaagacct
    23821 gtatccccat gcggtccgcc gatcacgaac cggccagtcg ggttgatgtg gtacttcgtg
    23881 gccgcgtcca ccatcgacgg tggaagcacc gggtcgatga tgcgcctccg cacctcagcc
    23941 cgcaagacct ctgtcggcac cttgtcgctg tgttgcgtcg atacgacaac cgcatcgatg
    24001 cgcgtcggtt ttccgtccac gtactcgacg ctcacctgcg acttgccgtc gggacgcagg
    24061 aaatcgagtt ctccggcccg ccgggcctcg ctcaggcggc gtaccagctt gtgggccatc
    24121 atgatcggca gcggcatcag ctccggtgtc tcgtcgcacg catagccgaa catcagcccc
    24181 tggtcccccg ccccgccggt gtctacgccc atcgcgatgt cgggagattg cgattggatg
    24241 gtgttgatga cgccacacgt tttgccgtcg aacccgaacg tcgcgtcgtt gtacccgatt
    24301 tcgttcacaa cggaccgtgt caccttcgga acgtcgacgt agcacgacgt tgtgatctca
    24361 cccgacacca cgcagagccc ggtcgtcacg agtacttcgc acgcgacgcg gcctgtcgga
    24421 tcctgcgcca gcacggcgtc cagcacggcg tcggaaatct ggtcagccat cttgtccgga
    24481 tgaccctcgg tcactgactc tgaggtgaac aggtgtttgt tttcggtcaa agcaacttcc
    24541 tccttacgcc gggcgcacgt ttggctggtc cctcgcctga cgaatgacgg cgaaccgcaa
    24601 ctctcctgcg agtacccagc ccgggaatcg acgtggacag gccgaagcta aagtttagca
    24661 tgccggccgg ttacgcaatc cggcccaacg cccgccgcta cttctcccct cgtggacttt
    24721 cgcagaggag tcgcgatgcc aatgtgatag ccgtcacggc tgtctgaaag accactccac
    24781 ggcgctccac attcggacgt ctatatctga taatgagcag ggaggcgacc cgatgctcct
    24841 gtttggcctc cgagacgatc tgcgcgaggc gtttcaccgc atccgccgca atccgcgcac
    24901 gtccctggtc gctgtcggca ctctggctct tggaatcggt gccgccaccg cgattctcac
    24961 cgttgcccac acctgtctca tggcgccacc gccgttccgc gagccggatc gaatcgtgag
    25021 agttcacgga ccgcgcacct cgtgggggtg gcagggcgtc agcggcgcgg acttcctgga
    25081 ttacctggaa gagcccggtc tattccaatc gggcaccctc gtcgggtatg cggaattcag
    25141 ttggactggt cagagcctcc ctggattcga tggggccgaa gttctgcggg ggtacgtcgt
    25201 taccgccgat tattttcgcg tgatggacca cccgatggcc gccggtcgcg gattcctctc
    25261 cggcgaagag cacgccgtcg tcatcagtca cgctctgtgg cagcgccgtt tcgccggccg
    25321 cggcgacatc atcggccagg ctgtcacgat aaatggcaca tctcacgtcg tggtcggtgt
    25381 cgcggggcgc ggcttcctga cctatggaag ttacgaggta atggcctggg tgccaatttc
    25441 accggacagg gagtggcgct cctcgcgcca gtacagttgt ttcgcgcggc tggctccagg
    25501 catcggcctg gaacaagtgc agcagcgcct cgacgtgctc aaccgccact tggccgaagc
    25561 ctatcccgcc accaatgcca aatacgcagc caaggtcgag ccgttccttc atgaagttcg
    25621 acaggaagtg cggccggcga tgctcgcgct gctggccggg gtactctgcc tgctcttgat
    25681 tgtcgtggcc aatgtcgcga gcctgctgct tgcacgcgcg gccggtcagg cgcgcgagat
    25741 ggccatccgc accgcccttg gcgccagccg cctgcggctc taccggatgg tgattgcgga
    25801 gagttgtctc ctggccctgg tggcgagcgt gggtggcgtg gcactgggcg cgtggctgat
    25861 taccgggctg cgcgaactga tcccggccac gctcgcggtc ggctgggcct tcgcgctcga
    25921 cgctcgcgtg ttcgccgccg cattcctact ctctgcactg gccggtctga ctgctggtgt
    25981 cgcgccggcg tttgagagct tccggatggc ggcgggcggc atcaggccga cgatttcgcg
    26041 cagccggctg ttacgtggta tcacaaccgt cgagatcgcc ttggcggtgg tgctggcgat
    26101 cggcgccggg ctgctgggga agagtttcct gcaactcctc gaccgtcctc tcggctatcg
    26161 caccgactcg ttactgggca tgcgcgtgcg tcttgtcggt gatcgctaca agagcatcga
    26221 tcaacgcgcc gcttactgga gcgaattgat ggagcgcgcc tcgaccctgc ccggcatcgc
    26281 caaatccgca atggtctccg atctgcccat gggctggcaa tacagcggcg gctccttcga
    26341 ggcggcggac aagccagtag cgcccggcga gacgaggccg cgagcacacc agatcgtggc
    26401 gtcacccggt tatttcgcca cactgggcat ccctctcctc gctggccgcg gcttcaacga
    26461 aggcgacggt cctaaatcgg aaccggttgt gatcgtcaat gactttctgg cgcagaagat
    26521 ctggccgggc gagaatgcca tcggaaagcg gatcaaggcc tgggacggaa agacgtggcg
    26581 acgtgtggtc ggggtggtgt accgggttcg gcacggcggc ccggcagacc agtacgagaa
    26641 ccagctctac gttccgtatc gccagggcaa ctccaacgtg atgttcctgg tagtgcgtac
    26701 gcatgtccca ccggaatcgg tggtgccggc gatccgcacc acgctgaaat tcatggaccc
    26761 caacgttccg gctttcgaga tccgtagcat gaaggcggcc ttcgaacgcg aaactgcttt
    26821 gccgcgtctc ccgatggcgc tcacggtcgg attcgcgggg ctggctgcgc tgctcgccgc
    26881 tctcggcctt ttcggcgtca ttgcctactg ggtctcgcgg cgcaccaagg aactggggat
    26941 acgggcggcc atcggtgcgc gccctgccga actgcgagcg ctggtcttgc gtcagggact
    27001 gcggatggcg gccatcggtc tcacggcagg tgtagcggtg tcgctgacag taatgcgcta
    27061 tcttcacagc cttctttatg gcatgagcga gcgcgaccct tggatttacg ccggagcgat
    27121 cgcgctggcg ctgggcacgg cgatatttgc atgctggctg cctgcgagcc gggcagcgcg
    27181 cgtcgatccc gcccgagccc tacgcgagga aggctgagga ccggtggctc cgccatcctc
    27241 ttgacgtgcg ccgatcaaat attagactat agtcatatat gaccgtagtc acaaatgggc
    27301 tccagtcgag acgcgcgcgg aagaaggccg ccacgcggtc gcaaatcata gctgcggctg
    27361 tccgattgtt ttccgagcat ggcgtcgagg cggtcaccgt cgaccagatc gccgccgcgg
    27421 ccgatgtcgg gaaaggcacc atctacaatt acttccagac caaggaagac atcgtcgttg
    27481 atttcatggt tgaccttgag cgctctgtgc aggcacaggt gcgcgatttc aagagtcgtc
    27541 agcgttccgt ggccggtgtg ctcaccaact ttgtccagac ccaattccgg atgaaggagc
    27601 actaccacgc gttcgtccgc gtattcctgg gacacatgtt cctccacacc gagcagtttc
    27661 tgccgtacat ggcggagatg cagaaggtgc tcgatcctcc gcttgaggct ctgtttggtt
    27721 cgcttcagca gcgtggagcg attcgctccg atgtggacct ccgcgagctt attctcatgt
    27781 tcaagaccat tcagctcggg ctgaccgcgc tctgggcgat tgaaggaccg ccgttccagc
    27841 aggtcgcgcg catcatcgag caggaaatca cgctcttctg tgaaggcctg gaggtcgcga
    27901 gatgatgatt cggctgatga ttagcattgt cgttctgggc gccttgatgg gcagccagga
    27961 ttcttcgccg gccgagggac ttttgagcag gcgttaccgg gacggcgagc gactgaatta
    28021 tttgatgaaa ggacgtaata acggccggac gtatgaagtt cgactgaccg ctgttgtcaa
    28081 gaagggcact gacggacagt tcgtcgaaga gtacgcgtgg tcggaccttg tggtcgatgg
    28141 tgttccacag cttctgacgc ccgcaagccg ggagtttcgg caggcggtga cgttggccgg
    28201 aggttcacca ttcacgatgc ccgatctttc caaggtgcaa ccgggcctaa tcgggccggt
    28261 caccgatctg ttgacgttct acgccgacct gttcctggct acacatgccg ggcaattacg
    28321 cgagcccggg gatcgcttct tctttgcgaa ccccactgtg ggctcgtggg cggacgggac
    28381 acgtgtgctg ataggggaag actcgattga cttcgatatc cacctgaccg cagtcgaccg
    28441 gtcgcgcggc gtcgccacgc tcctggtcaa gcacgtgccg cccaaggcgc caaagattcg
    28501 gattcccgcc gaatggatgc gagagccggt ggccggcgtg ccgaacaact gggtccaggt
    28561 gagaaaggca gcgggcgcat acatcggcgc cgtcggcaag gagacgttcg acgtcgaact
    28621 gacgatcagg ttgtcttccg gcgagatttt gtcggcaacg atggacaatc gcgtcgaagc
    28681 catggagcgt gaatgtgtcg acgttgagct gtccaactgc ggcgagcggc gtcccgatcc
    28741 gacgattcgc cacatcgaga tgtcgctgac caactcagga cgataggaaa aatcaggtcc
    28801 cgcctgcggc ttccaaactc actggccaca ccatctcggg ggtcgcggat ctcagggagc
    28861 cagccggcgc cgccatctcg cgttgccggc agccgcgccg agcaccccga tgggccgccc
    28921 caccaccgga agcaagacca ggacccagat ggcgtccggc aggtttacgc cgacggggta
    28981 gcctagttca ccgtcaccga gcgtccaccc atcgaccgcg taccggtgca tggcttcggg
    29041 tagcgtgatg gcgtagacca gcagcgtgcc gatcagcgcc gtccacaccg cggcttggac
    29101 acccgcgcgg aaggaccggc cagccgcggc ggccaccgcc gcggcagcga agaagatcac
    29161 gatgggcgcg aatattaccc agatccaagg tccggcgtac gtgttgatgg tgaggcgcga
    29221 tgtcacgagg aacccaagac cgagcgtcag ggccgcgccc gcgccaagtc cccgtgcgag
    29281 acggctcgtg gtcagcccgt gcggcggggt cagcgcaagc cacaggcagc ctgtcaacac
    29341 gacggcgaag gtcaccgccc caatgggcgg gagatgctct gcggcagtgg ggtgcttgac
    29401 caggaagtat gcggtaacgg cgatgcacgc agccacgccc accacaccgg tgcctgtgat
    29461 ggtcggaccc ggcatcgcct ggcgcacccg gatcgagcgc gccgccgcca gcgtggccat
    29521 ggcgccgacg agcgcggcca aggtgaccgc gaagacccgc atcgcgggca aggcgtagcc
    29581 gaccaccggc caggccgtcg ccaccgccgt cgcggccact acccccgcca tgagcacggg
    29641 catccggctg ctgcgcggcg ggaatatcgc aacccgggcg cagccggcgg cgaaccacca
    29701 ccgcgatgag cgattccgca cctgggccag ttccgccgtc atcgccgcac cccagtcgcg
    29761 gcggtcgtcg ggcagcgtgg caacccccga agccagcagc agagccggac catccatggc
    29821 ggacacactg gcccctgccc tggcccacca ggcacgccaa agcagccgga ccgcgatcac
    29881 ccagagcgta gcaaccgccg cgaccatcgc cgcggaaaac acatactgag actggaactc
    29941 cgggggatcg cccatggcga acacgatcac ccagaatgcg gcgaacaccg cggctacggc
    30001 cgcggacacc gccaactcgt acttgcgctc cgtacgcgtc atgcgccctc caatcgcgct
    30061 cgcagcctgg cccgcttcgc cttcgcggcg cgggccggcg tcgggagttc ggtaactagt
    30121 tcgaggccct tgccggtcag acggtacagg tgccgcggtg gacggccaag cggcgcatcg
    30181 ttctcccagg tggtctccaa cagtccccgg tcagccagtt gcatgaggat ggggtacatc
    30241 gagccggctt tgaggccggt ctgccggcac aggtcgtagc catagcgcca cgctggggga
    30301 tcttccgcta gagcgaacag caccgcggtg gtctgcggcg aagggcgacg gggcatgaca
    30361 caagtctata tagatagagt tgtcatgtca agtaggtctg ggggacaccg cgccccgcac
    30421 ccccgccgcc acctggtaat cgacttcctc tcgaggggcg aacgagttgg cactggttcc
    30481 gagtagcagc gatcctagag ttggaatgcg ccggtctaca cttcgccgtt gatcgcggct
    30541 aatttggctt cttctcttgg accccgtact ttagcaggac ctggagcacg ttatcgtgtt
    30601 tcatcgtctc ggcccaagcc ctcggcgtcg cccacgattc ggcacccgcc tccaccgggt
    30661 cggcaccacg ctcaagcagg agtttcacca actcggcccg gccccaacgg caggcccaac
    30721 caagcgcggt gctgttgagc aggccatcgc gaagatcggt tcgtccgccc gcgtccagca
    30781 agacagttgc gaaagcgagc cgttcctctt cggttacgtg agggcgcgct ccggcgacgc
    30841 tgtgaagaat ggtcaggttg aagccatcac ccgagcgtcc gaggatgttc gggtcgcaac
    30901 gctcgagaat gagcttcagg cagtcgagtt gagcgcctcc gtcgctccac atacggatcg
    30961 gctgttccat gatgtggaac caccacgggt cgtccttggg ccgatcgatg tactccagcg
    31021 ccatgcgtac gatctcggca tcgccgccac aggcgccgcc ccacaacagt tgctcggcaa
    31081 gcgtatcgcc gcccacgcct tcgctcccgt actttgcttc gccggccagc atctttcgag
    31141 ccaactcagt ttggcgaaaa agacccgcag tggtggcctc cggaaccccg ccgtaacgct
    31201 gcaatagttc gaccattttc cagtcgcgtt tatcataggc ctgaaagacc gggtcaccgc
    31261 tggcgtagac tttgccgttc gggtccgctc cgtgtttcag cagaatctcc gccaattcgc
    31321 atttgccgaa ctgggcgcaa tggtagagtg gcattcccca ggtgaaggcg atgctgtcgg
    31381 cgtccacgtc ttccatgcgc gtgcgttcgt tcgggtcgaa gccaagtttg agcagcaatt
    31441 gcagaatatc ggcgcgattg tagctcgcgg cgatgcgaag catgcctccg gtcccctcaa
    31501 tcgggttggt aagcgagcct tgcgcgtgcc gcgcccgaag ccactcggcg tccccgagcg
    31561 ccacagcagc ccacggtgtc atttccgcgc ccgctcgtac aagccggttc gcgacaacag
    31621 cgtattccgc actcttgttc gcgtccgacg agtgcgctgc agtatccagt ggtgtctgct
    31681 cggtccagtt gcgccccttg gcattcacgt ccgcgccgcg ctccgtaagc caattcacca
    31741 tttggacgtt aagcttggct gcggcaacgt gcaggggtgt ccatccatga tgcagaaggc
    31801 gtgcctggat cagtgaaggc tctgactcca tcaagccgat ggctcgatca ttctccccag
    31861 aagcgatcgc gccaaacagc acttccggat caggggctcc gaggccggcg ctgttggttt
    31921 cgcggcgccg ccgttcaacc tctttgatag tcacgacaat ctcgtcgtat ccgcgatccg
    31981 tcgcgatcac cagtggtgtg gttgcatccc gatgtgggta caccccagtc cttgcattgg
    32041 cgccgtgctc catcagcatg cgcaccatcg tgtcatcgcg ccgcaagact gcatgccgca
    32101 gagcggcgct gttcacgagc tccggccgat gtttcagcat cacgcgaagc tgggcgatgt
    32161 caccgttccg agcgacttcc tcgaaccgct tgaccgtgac accgtccacg aacgccctca
    32221 atttgggcca actatcgaag ccgtacgcac gagcgaggac gagttgcgcg tcatgcaatg
    32281 caaacgtgac cgggcttgcg gcgcggtaat gtgcattgac ctcagcgact gctgcggcat
    32341 ctccggtggt aaatgcttcg agcagttcct tcgcctgacg cttgagttga tcaagatccg
    32401 gatgctcgcg aagcttgcga gtgggcagac tgcgattcac gatgagctcc tttctctgcg
    32461 gctgcgctat ccgcgccggc cgctgcacct ggtgtccgct cgctcaggca gaaaagagtt
    32521 cagtcaaacg actcagtcgg cgtacaacgc tcagggggga cgcagtcctt cccgcggaca
    32581 cggaggcgtc ctgacgcgcc actgacatct tagcagggcc aaccaccgcg aaatgtcggc
    32641 cagcgtttac cgatgactcg gcatcaccgg tggggccact ctgatcaaat gactgccaaa
    32701 ccggaagttg gtgctctggg aagtagttct tggctaccag gggccggcca gcttggcagg
    32761 actctccgaa acgctgcgcg ataagcttgc actgaggtac tgccgcccgc ccttcatctc
    32821 cgcaccaccg ccgccacctc gtaatccacc acctgcggta cgtcgaatct gaccaggccg
    32881 ccccggtgct cgaatggcag ccgcctgccg gaacggagcg ctcgcacctc agcgatccgt
    32941 atgtcctccg gcacccgcaa cgtgatttcc tgccggccca cagggtagaa ctcgcgcacc
    33001 caaccgccaa gcatgtgggg gttggtgtag ttcacaaggt gaatcgcata gcccggcttc
    33061 gtctcccacg cgaagatctc gatgactcca tcgccgcgca cctccaccgg cgcaggaccc
    33121 tgagctgtgc ggccgccgcg aacccatcgg atcgcgtttt gcagaagccg gtccaagtcg
    33181 agattactcg tgcgccagca cgtgcgatcc acatcgccgg ggaagtaggc gacgcggcct
    33241 ccgccgtccg tttctctcag aacaagcgcc gactcatccg tcctcagcgt tcgcgggtac
    33301 accatctccg gcggatgcga cggaaacggc ggcatgtagc tcaacggcgc ttcggccttg
    33361 ccggtcaccc tcaccgggac ccggtagacc gcgccgggca gcagttccgt ggggccaatg
    33421 ccatcgaaaa tctcgtggtc cgccttctcc actctgccgt aggaattccg cagcgggccc
    33481 tgcgcgtcgc cggtcgacga caccccgagc acttccgcca gcccgaagtc cggccggcgg
    33541 tctccccatt cgttgtacag gctcgtttcg tacgtcgcga gcaacgatcc gccccgcccg
    33601 acatagtccc ggagctgcat gcactgccgg tctcccagat tcgcgacatt tgggaggatc
    33661 agcgcccggt attgccgtaa cgtctctggg gagagatcgt tctcgtgtac gaagtcgaac
    33721 gcgtgacggc cctcgagcag cgcgtagtac agcccctgga aatgatcggt cgcctcgcgc
    33781 cggtcccggt acgcccgtga accttcgacc ccgtaaaatg ccagcgtccg ctgcgagtag
    33841 accatggcga tgtcggcgag cggccgcacg ttgcggaagt gctcggagtg gcgcgccgtc
    33901 cactggtaga actcccggcc tgcgtcccgc caccggttgt ctaccggcga cgcgccgagc
    33961 cagtgatacg tcggagcggc cccggccgcc accgtcgccg ccagccagag cgtcgcctcc
    34021 tccggcgact tgctcgtgtg ccgccacgcc atgtagttcg tgctggcata cgccccgcag
    34081 acgtttgtca ccgtgcgccc tttcatcgcc gagcgcgccg cccgcacctg ctgcgcgcaa
    34141 taccagagcg ggttcgtgtc gcctccgcgg tcctggccgt ccaggttgaa ccactcggcc
    34201 gcttccgcca gtttggtcag atccttcact gccctgaggt tcgtatgcag gttcccatag
    34261 aagacgccgt cgcgcttctt taccttggcc gtttcgtccc agagccgcca gatctccagg
    34321 atccgttcca tgtggaggtc gaagaacttc cggtatctcg gatcgctcgg cgccgttcgt
    34381 tccggggtcc cgccggtctt ctcgcgatag acgcgcgcgc agttctcgca gtagcagagc
    34441 accggcgcgc cggtggtcgg ccagccgttc gtgaagaatc cgtcgatgtc gtaccggctg
    34501 ctcacctcgc ggaagatctt cggcaactgc tcggtgaaat acgggctgaa catgcaggtc
    34561 cggtacagcc atgtggactc cggatcggtg cgcggcttgc cgtccgcatc gcgctggaac
    34621 cactccggat ggtcccgaag ggcgtcctcc cacgcgaaat tcgggtccat gcgcgctacc
    34681 acacgcattc cacgccgtct caccgcggcg atgaactcgc ccagaacgtc cctctctcct
    34741 aagaactggc tgcggtggtg gtacggcgcc gttgtggggt agtaggcgac gattcctccc
    34801 ccgtttagca ggacgacgtc gactttcagg ctcgcccagt actcggccca ctcctcgacg
    34861 tccagacccg tcaggtcttt ctcgttcagg ttggtttggc cgcagcgttg aatctgccgg
    34921 taccacggct caccgtcatc ccccgcggcc tgggtcttcg ttgccgcaat caatgttccc
    34981 gcgacgccca gaaactctcg ccgtgataca cggtccgtca tgattccttc ccttccgcca
    35041 ccgggggaca ggcttactct gtcctgagcg cactggggac aggcccactc tgtcctgagc
    35101 gcagttgtgc tatccgggac atgggtaaca gaagttgcta accacatggg ttacactttt
    35161 tcctgttcct catcatcatg atgctcagtc attggtcgcc gcgcaaggag cccggagggt
    35221 gactggagcg gcggccaatg actgtcgatc ttactcgccg ctcggctcgg gccttctcgt
    35281 tcgtccaaca gacccagcaa caccggcccg aaccacacac gccagacgtt gtcgtcgatc
    35341 tcctccaatc cgaccacctc tccagccaac gcccgcccca cccgacagcg ggcctgcttc
    35401 cagcggatct ttcccccttc atcggccaac ctcagcggcc agcccgccgg atattccacc
    35461 tccggcagtt tccgcggaaa ctcacgcgcc gaccgctgat actgctcggc cggtgtctct
    35521 ccccccaacg cctcatgcgg ccgttcgtgg ttgtagatcc ccaggaactc cgcaaagcgc
    35581 ttctgctggg ccgccacggt ggccgccggc ggcagcgccg tttctgcctt caacgtccgg
    35641 tgcatccgtt cgtgccgccc gttctgctgc ggttcgcccg gctcaatccg ttccggcaca
    35701 atacccaact tcacccacca cacgctcaag gcgctcaacc cgcccacgcc ggtggaggca
    35761 aacggactcc cgttgtcact ccgcatcctc tccggcaatc cgtagtggcg aaagactcgc
    35821 ttcagctcgc acttcaccgc ctccgtatcc gtgctgggcg tcgcccggca gcagagcaga
    35881 aatcgggtgg ccgcatcact gacggtgagc ggatcgcagc ggctcccatc cccgcatcgg
    35941 aaccagccct tgaaatcgat cgaccagacc tggttggcgc ggctagcgtg cgccaacggt
    36001 tggttggacg gcgtcgcatg gcgacgcttg gaccgcttcc cattcaatcc gtgacgcttc
    36061 aaaatgagcc ccatcgtgct ttccgccggc cagctctttt ccggcatctt tcgctccagc
    36121 caccgccgca gcttccgcgg cccccaactg gggtgcgtcc cccgcaaatc gcttacccat
    36181 tgctcgatct cggcgctggt ccgcccagcc tgcaccagcg gccgccgcga ctgatccttt
    36241 aacccttcca gaccgctttc ctcaaatctc tccaaccact tgtacacggt cttgcggctc
    36301 accccaaacc gggctgccaa ctccgtaatg ctgtcgccgc cttcccgcca acgctcgatc
    36361 agccgaaacc gctcttccat cacatcgctc actttccagg gcatcgcttt gaaggatgcc
    36421 ccaaatctgt taccgatgtg attggcaact tctgttaccg atgtggtcgg ttcgtacc
//
LOCUS       DATIFQ010000096         3348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_30663, whole genome shotgun sequence.
ACCESSION   DATIFQ010000096 DATIFQ010000000
VERSION     DATIFQ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30663"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VLE22_04350"
     CDS             <1..200
                     /locus_tag="VLE22_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13665.1"
                     /translation="GKSAGGAPQMQLSEQWLVERKPGPGNVMVETSSVAKPTLDYPAR
                     LGAYQKIFEVVCSGSCKSEEK"
     gene            330..1412
                     /locus_tag="VLE22_04355"
     CDS             330..1412
                     /locus_tag="VLE22_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HSB13666.1"
                     /translation="MRVACLIVLVALSLLAQTPPLQQPWEGVPERFRHLPIGQLKAPS
                     SLKEWQKQRQQVKKIVLQSLGDLPARPSPLKVRTVSTDKKEGYTIEKFVFDNGVDSMV
                     PGYIAIPDKRGGRLPAILTMHGHGSSKENMFGYQPTSQNVAEMLVGKGYVVLGIDNYF
                     NGERKGTGPAGELETMAKGSDQEMSLFKLNLWLGRTLWGMMLRDEQIALDYLSSRPEV
                     DHDRIGAQGMSMGSTRSWWLAAIDDRIKAVVGVACFTRYEDLIAIRALSAHGIYYFVP
                     GILKHFDSEGVMALLAPRPFLALTGDRDAGSPPEGMKTLEQALKRFYGLYGKPDAFQS
                     IIYPETGHVYNDDMKLRMVEWFGKFL"
     gene            1465..>3348
                     /locus_tag="VLE22_04360"
     CDS             1465..>3348
                     /locus_tag="VLE22_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13667.1"
                     /translation="MPTLSIVSQAAGVTAGRQGGGETQGRSKKFTPVDLSPYYNAGPV
                     DFGPREKARGLSGKSRGDGLIYMPAGRQTFRGIPFRLGRQGIREKSWIWLSTRTGKAA
                     PGVEVPLQDKAGFICFAHFCDWDPNEIPDLDAEAVEKVGQTLTEAILIYSDGSEVPFP
                     IRRRIEVNAPSIRWGFPGYACVSHYQDSTRKSRNELDELKDWGWFRLQSDVLEGNYEE
                     DATTGAPKPSLWLFALPNPHPEPSLTALRLRSVSEEPLLVCGITLFHGRQHPLRYGRL
                     TLYRLTLPDTAGRWSVDIDLGLVARSYRLGKFEPQEWLVAPYAGLGSRSEPGSGRHLY
                     VEVTASPEATLLLRDDRGGRQFEFALDKVQAGEEMPGTPAGPRIEVLETEKTWIHGKV
                     TDAATGRPTPVRLAFCSKEGRYIPPYGHRTEVDAGTFKDWGADLKLRDTSYAYVDGTF
                     QIELPVGDVYVEINKGFEYAPLRQKLSIQRGQRELSLEIARALDFRSLGWVTADTHVH
                     FLSPSTAVLEGQAEGLNIVNLLAAQWGDLFTNVGDLGQDELMSRDGDTVVWMGTENRQ
                     HILGHLGVLGKVVFPMSAGGPRESYHGDPLWDTLAEWADACRRQGGLAVSVHFPYPAG
                     EQAA"
BASE COUNT          683 a          951 c         1078 g          636 t
ORIGIN      
        1 ccggcaagtc cgccggcggc gcgccgcaga tgcagctcag cgagcaatgg ctggtcgagc
       61 ggaaacccgg cccgggtaac gtcatggtgg aaaccagtag cgtcgccaag cccacgctcg
      121 actatcccgc ccgcctgggc gcttaccaga agattttcga agtcgtttgc agcggcagtt
      181 gcaagtccga agagaaatga cacacgcctg accctactgg caggcgccgg ccgagagact
      241 gtttcgagtg tctggacggc aacgtagttc gggtggtccg cgcagcgttg tccaagatgt
      301 cgtgattccc atcataggag gagtaacgca tgagggttgc ttgcttgatt gtgcttgtcg
      361 cgctgagcct tctggctcaa actccgcctt tgcagcagcc ctgggaaggg gtgcctgagc
      421 ggttccgtca tctccccatc ggacaactga aggcgccgtc gagcctgaag gaatggcaga
      481 agcagcggca acaagtgaag aagatcgttc tgcagtctct cggagatctg ccggcacggc
      541 cttctccgct gaaggtccgc acggtatcca cggacaagaa ggaaggttac accatcgaga
      601 aattcgtatt cgacaacggg gtggactcga tggtgcccgg ctacatagcc attccggaca
      661 agagaggcgg gcggctgccg gccattctca caatgcatgg ccacggaagc agcaaggaaa
      721 acatgtttgg ctaccagccg acgtcgcaga acgtagcgga aatgctggtc ggaaagggct
      781 acgtggtcct cggcatcgac aactacttca acggcgagcg caagggaacc ggtccggctg
      841 gtgaactgga gaccatggcg aagggctcgg accaggagat gtccttgttc aagctgaacc
      901 tctggctggg acgcacgtta tgggggatga tgctgcgcga cgagcagatc gctttggact
      961 acctgtcctc ccgccccgag gtggaccacg accgtatcgg cgcgcaagga atgagcatgg
     1021 gcagcacccg ctcctggtgg ctggcggcca tcgacgaccg gatcaaggca gtcgtcggag
     1081 tggcgtgctt tacccgatac gaggatctga tcgccatccg tgcgctgagc gctcatggca
     1141 tctactactt cgtcccggga atcctgaaac acttcgacag tgaaggggtg atggcgctgc
     1201 tggccccgcg cccttttctt gctttgaccg gagaccgtga cgccggttcg ccgcccgaag
     1261 gcatgaagac gctggagcag gcgctgaagc gcttctatgg tctctatggc aaaccagacg
     1321 cctttcaaag catcatctac ccggaaacag ggcacgtcta caacgacgac atgaagctga
     1381 ggatggtcga gtggttcgga aagttccttt gaagagatga agaatcggac aagaagacac
     1441 tttctagaga ccgtggggac tggtgtgcct acgctatcta tcgtttcgca ggcagccggc
     1501 gtcactgccg ggcggcaagg gggaggtgag acccaaggac gttccaagaa gttcacgccg
     1561 gtggatctga gtccgtatta caacgccggg ccggttgact ttggcccgcg cgagaaggcc
     1621 cggggcttga gcgggaaatc ccggggcgac gggctgatct acatgcccgc gggccggcag
     1681 accttcaggg ggatcccatt ccggctggga cgtcaaggaa tacgcgagaa atcctggatc
     1741 tggctgagca cgcggaccgg gaaagcggcg cctggagtgg aggttccact gcaggacaag
     1801 gcgggcttca tctgttttgc acatttctgc gattgggacc cgaacgagat tccagatctc
     1861 gatgccgaag cggtggagaa agtaggccaa acgctaactg aggcaattct aatctactcc
     1921 gatggcagcg aagtgccgtt cccgatccgg cggcgtatcg aggtgaatgc tccttccata
     1981 aggtggggct ttccgggcta cgcctgtgtt tcccactacc aggacagtac ccgcaagtcc
     2041 agaaatgaac tcgacgaact caaggactgg ggatggttcc ggctccagtc ggatgttctc
     2101 gaaggcaact acgaggagga tgccacaact ggcgccccaa agccgagcct ctggctattt
     2161 gcgcttccga accctcatcc ggagccgagc cttacggcgc tgcggctgcg atctgtcagc
     2221 gaagaaccgc tcctggtctg tggaatcacc ttgtttcacg gccggcaaca tcctttacgg
     2281 tacgggcggc taaccctcta tcgcctgact ttgcccgata ccgccgggcg atggtcggtg
     2341 gacatcgatc tcggactggt ggctcgatcc taccgcctcg ggaagtttga gccgcaggaa
     2401 tggctcgtcg cgccctatgc gggtttgggc tcgaggtcgg agccggggag tggccgccac
     2461 ctctatgtcg aagtgacagc cagccccgaa gctaccttgc tgttgcgaga cgaccgcggc
     2521 gggcggcagt tcgaatttgc tctggacaag gttcaggccg gagaggaaat gccgggtacc
     2581 ccggccgggc cgcgaatcga ggttctggag accgagaaaa cctggatcca cggcaaggtc
     2641 acggatgctg ccacaggcag gcccacgccg gtcagattgg cgttctgttc taaggaaggg
     2701 cgttacattc ctccttacgg ccaccgcacc gaggttgatg ccggaacgtt caaggattgg
     2761 ggcgccgatc tcaagctcag ggacacgtcc tatgcttacg tggacggcac gttccagatc
     2821 gaacttcccg tgggcgatgt atacgtcgaa atcaacaagg gattcgaata cgctcccctt
     2881 cgccagaagt tgagcattca acgcgggcaa cgcgagctga gcctggagat tgcgcgagct
     2941 ctggattttc gttcgctcgg ctgggtgacc gccgacactc acgtgcattt cttatcgccg
     3001 tcgaccgcgg tattggaggg acaggcggag ggactgaata ttgtcaatct cctggccgcc
     3061 caatgggggg acctcttcac caacgtaggc gatctgggcc aggacgagct gatgtcgcgc
     3121 gatggcgaca cggtcgtgtg gatggggacc gagaataggc agcacattct cggtcacttg
     3181 ggtgtactcg gcaaggtcgt ttttccgatg tccgccggcg gcccgcgcga gagctaccac
     3241 ggcgacccct tgtgggatac tctggccgag tgggccgacg cctgccgcag gcagggggga
     3301 ctggccgtgt ccgttcactt tccttatccg gcgggcgagc aggcggcg
//
LOCUS       DATIFQ010000097         6199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_30785, whole genome shotgun sequence.
ACCESSION   DATIFQ010000097 DATIFQ010000000
VERSION     DATIFQ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30785"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..2367
                     /locus_tag="VLE22_04365"
     CDS             343..2367
                     /locus_tag="VLE22_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSB13668.1"
                     /translation="MRRKRGSPSSQYGSKRAEDLRSKEMERTGALLELYMKAPQLTDQ
                     QICECALEEAVKLTDSEVGYLHELSVDQTVLTLSTWNEEARKQCSATHTDHYPLSEAG
                     IWADCVRLGRPVVHNDYPAIEHKKGLPDGHVQVSRHLSLPVMENGRISFVLGVGNKQA
                     GYNADDIIQLQQISSELHKIMVRRRAERALRASETRYRVVADNTYDWEFWLDPDGRFI
                     YSSPSCERITGYSAAEFEADPGLLVRLIHPDDRPRFEAHQRKVCVDRVVAEEEFRIVR
                     ADGAVGWIGHLCGPVFDEAGHFLGVRGSNRDISERKLAEETLRRSEERYGTLVRSSPY
                     CIHEIDRQGRLTSMNPAGLAMMGVRDECAIVGMPYLDAVADEDRGRIGQLLQAALEEG
                     QRSEFEFRAGNGLYFASSFVPIRDAEGAVIRVMGLTQDITARKKAEASLLDYQERLRC
                     LTSELSRAEQQERRRVAGLLHDDVIQALALSRIRLSELRQSLVAEQQGLLDRTRELVE
                     DAIHGTRSLMSQLSPPILHELGLDPALDWLTEQIAGREKLAWRFVSDGQPKLLDDDGK
                     VLLFTAARELLLNVVKHARAAQVTVRSSRENSAIQISVEDDGVGFDTRFKSRCCGESG
                     GFGLFSIRERLGYLGGCCEIHSEPGKGTRAVLALPLKQDGAHEDSHHTGG"
     gene            2339..2986
                     /locus_tag="VLE22_04370"
     CDS             2339..2986
                     /locus_tag="VLE22_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB13669.1"
                     /translation="MKIRIILADDHRIIRDGIRVLLAGLDGMEVIADVGDGRTAVKLA
                     AELKPDVIVMDIGMPDLNGMEATRQIKAQDPGVQVIALSMHRDVRFVSEMLRAGASGY
                     LLKEAAFEELARAIQAVTRHETYLSPSISGVVVQDYVSRLGEARPAGSAALTPREREV
                     LQLISEGHSSKEIAFRLHVSLKTVETHRHQIMSKLGLRSVAELTKYALREGLTAL"
     gene            3338..3649
                     /locus_tag="VLE22_04375"
     CDS             3338..3649
                     /locus_tag="VLE22_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13670.1"
                     /translation="MLLRVSIPVEAGNAAAKAGTLGSTVEQIVANLKPEAAYFFADDS
                     GQRSGSIVFDMKDTSEIPAVAEPWFLAFNAKVSLRPIMNPQDLAKAGPSIGKAAKQYG
                     K"
     gene            3764..4576
                     /locus_tag="VLE22_04380"
     CDS             3764..4576
                     /locus_tag="VLE22_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13671.1"
                     /translation="MLFSDLALARRLERAEGHACVQFAEARRRLFPDSGAEWIECAGA
                     YAIFDGIDSPVTQSFGLGIFEELSADSLDVIERFFVSHGAPVLHEVSPLAGVAALGLL
                     CARNYRPIEISNVLYQPVEKPAADHSGNITVRVIGPQEARLWADIGARGWAYEHPELL
                     DFLLQLAPISAARRHSVCFLGEVDGKPGAAGVLCMHNGVALFGGSATIPELRRRGLQA
                     ALLHGRMRYAFDHGCDLAMMVAAAGSDSQRNAERKGFRIAYTRTKWRLIADR"
     gene            complement(4595..5095)
                     /locus_tag="VLE22_04385"
     CDS             complement(4595..5095)
                     /locus_tag="VLE22_04385"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSB13672.1"
                     /translation="MKREELRALQAPLKQRYREDPKAAVVTLTAEGWLGEGVSCRVET
                     FGGLVEAGLHPATGGSGQEACSGDMLLEALVACAGVTLSAVATSLGLELRGGHLRAEG
                     DLDFRGTLGVAKDVPVGFREIRLRISLDTDASREERQKLLELTERHCVVYQTLRNGPR
                     LTVDVD"
     gene            5262..5705
                     /locus_tag="VLE22_04390"
     CDS             5262..5705
                     /locus_tag="VLE22_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB13673.1"
                     /translation="MIELAAELRTVVERAAVRFRSLREDDVTRDLGAGRWVKKEILGH
                     LIDSAANNHQRFVRAQFTWPFVWPGYDQQTWVPLHHYRDRPWTEVVDLWVALNRHVAA
                     VIEFVPVEKLQTPCTIGDYEPASLEWWMRDYLRHLKHHLEQFEVA"
     gene            complement(5911..>6199)
                     /locus_tag="VLE22_04395"
     CDS             complement(5911..>6199)
                     /locus_tag="VLE22_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HSB13674.1"
                     /translation="INRYIAGAKLGLHQDRDEKDAWAPIVSVSLGLPAVFLWGGKRRS
                     DPVRRLRVENGDVAVWGGPARFVYHGVAPLKDGQHPLTGNARINLTFRKVF"
BASE COUNT         1205 a         1877 c         1983 g         1134 t
ORIGIN      
        1 acggcaagtc cccggtcatg ggcatcgacg tctgggagca cgcctactac ctgaagtatc
       61 agaaccgccg cgccgaatac atcgccgcct ggtggaacgt cgtcaactgg aaagaaatcg
      121 aaaagcgctt ctgatagctg acggctgatc gctaactaac agctgacgca gggagcggcc
      181 tctctttgct cctctgcttc ttccgcgtcc tgtgtggtga atcaggtctt tccgagcccc
      241 accgcaggga gcggtcaaca aaacccgcga ccacgccccc gcgcgcgagc cgtcggtgat
      301 agctgacacc tgaaagccga tagctgtatc ctaaatagcg tcatgagacg gaagcgcggg
      361 agtccgtcgt cacagtacgg cagcaagcgg gccgaggact tgcggtccaa agagatggaa
      421 cggactggcg cgttgctcga gctctacatg aaggcacccc aactgaccga ccagcagatc
      481 tgcgagtgcg ccctcgaaga ggccgtaaag ctcaccgata gcgaagtcgg ctacctccac
      541 gagttgagcg tcgatcagac cgtgctcact ctctcgacct ggaacgagga ggcccgcaag
      601 caatgctccg caacccacac cgaccactac cccttgtcgg aggccggtat ctgggcggat
      661 tgcgtgcgcc tggggcggcc ggtggtccat aacgattacc ccgcgatcga acacaaaaaa
      721 ggtcttccgg acggacacgt gcaagtctca aggcacctga gtctgcccgt gatggagaac
      781 ggccggatca gtttcgtcct cggagtcggc aacaagcagg ccggctacaa cgcggatgac
      841 atcatccagt tgcagcagat ctctagtgaa ttgcacaaga tcatggtgcg gcggcgggcc
      901 gagcgggcgc tccgggcttc ggaaacccga taccgggttg tggccgataa cacctacgac
      961 tgggagttct ggctggaccc cgacggcaga ttcatctact cgtctccctc ctgcgagcgg
     1021 atcaccggct acagtgcggc tgaattcgag gctgatcccg gcctcctcgt gcgcctgatc
     1081 catccggatg accgtccgcg atttgaagca catcagagga aggtttgcgt cgaccgggtc
     1141 gtggccgagg aggagttccg cattgttcgc gcagatggcg ccgtgggatg gatcggccat
     1201 ctgtgcggtc cggtttttga cgaagccgga cactttctcg gggtccgcgg cagcaaccgc
     1261 gacatcagcg agcgaaagct cgcggaggag acgctgcgca ggagcgagga gcggtacggc
     1321 acgctcgtgc gaagctcacc ctactgcatt cacgagattg atcgccaggg acggctgacc
     1381 tcgatgaacc ctgccggcct ggcgatgatg ggcgtccggg acgagtgcgc gatcgtcgga
     1441 atgccctacc tcgacgccgt ggcggatgag gatcgggggc ggatcggcca actgcttcag
     1501 gctgcgctcg aggaggggca gcggtcggaa ttcgaattca gggccgggaa cgggctctat
     1561 ttcgcatcct ccttcgtgcc catcagggac gcggagggcg cggtgatccg cgtcatgggt
     1621 ctgacccagg acattaccgc ccgcaagaag gcggaggcga gtttgctcga ctaccaggaa
     1681 cggctccgct gcttgacgtc cgaattgtcg cgggctgagc agcaggagcg gcgccgcgta
     1741 gcgggcttgc tgcacgatga cgtgatacag gcgctggctt tgtcccggat caggctatcg
     1801 gaattgcgcc aatcactcgt cgcggagcag caagggttgc tggaccgtac ccgcgagctg
     1861 gttgaggacg cgatccatgg cactcgctcg ctgatgtccc agctcagccc gccgatcttg
     1921 cacgaactgg gcctggaccc cgcgctcgac tggctcaccg agcagatcgc cgggagggag
     1981 aaactcgcct ggcggtttgt cagcgacggg cagcccaaac ttctggatga cgacggaaaa
     2041 gtccttttgt tcaccgccgc gcgggaactc ctgctgaacg tcgtcaagca cgccagggcg
     2101 gcccaggtaa cggtgcgctc cagccgggaa aacagcgcga ttcagatctc tgtcgaggac
     2161 gacggtgtcg gcttcgacac ccggttcaag agccgctgtt gcggcgagag cgggggcttc
     2221 ggtcttttca gcatccggga gcgtctgggg tacctcgggg gctgctgcga gatccactcc
     2281 gaaccgggta aaggcacgcg cgcggtcctg gcgctgcctc tcaagcagga tggcgcacat
     2341 gaagattcgc atcatactgg cggatgatca ccgcatcatc cgggatggca tccgggtcct
     2401 gctcgccggc ctcgacggca tggaagtgat cgccgacgtg ggcgatggac gcaccgccgt
     2461 gaagctggcc gctgagctca agccggacgt gatcgtcatg gacatcggca tgccggatct
     2521 gaacggcatg gaagcgacgc gccagatcaa ggcgcaggat cctggcgtgc aggtaatcgc
     2581 actctccatg catcgcgacg tgcggttcgt ttccgaaatg ctgcgtgccg gcgcctccgg
     2641 ctaccttctc aaggaggcgg ccttcgagga actggcccgc gccatccagg ccgtgaccag
     2701 gcacgaaacc tacctcagtc ccagcatcag cggcgtggtc gtccaggact atgtgagccg
     2761 gcttggcgag gctcggccgg cgggatcggc cgccctgacg ccgcgggaac gcgaggtgct
     2821 tcagttgatc tcggaaggcc acagcagcaa ggagatcgcc ttccgcctgc acgtgagcct
     2881 gaagaccgtg gagacgcacc ggcaccagat catgagcaag ctgggcctcc gcagcgtggc
     2941 cgagctcacc aagtacgccc tgcgcgaggg gctcaccgcg ttgtagcgag tccgcgcaac
     3001 ccatctcagg attttcgcac ccccggatcg ggggatctcc cgatgcgcac cgggactacc
     3061 gtgtgtcaat ttggtggtgc gggtccgtgg gaggccaaga aacaccagga agcaaatcgg
     3121 ctgggaaacc gaatttgggt tctgggggag gcggtggagg atgtggatgt cccttgatct
     3181 gggcggaggc tgcgcgatca tccacacgcc gtagctgggt acgcaacggg ggtggccgga
     3241 gaaccacccc cacgccgccg ggaagcagcc cttccacaca ccattcgggt ataatcgtcc
     3301 cacgcctttt ccttgaggga ggaaaaccca aatgcgaatg ttgctgagag tatcgattcc
     3361 cgtggaggca ggaaatgccg ctgccaaggc cggaaccctg ggttccactg tcgaacaaat
     3421 tgtagccaat ctcaaaccgg aagctgccta tttcttcgcg gacgacagcg gccaacggtc
     3481 gggctccatc gtgttcgaca tgaaggacac atcagagatt ccagcggtcg ctgagccctg
     3541 gtttctcgcg ttcaacgcaa aagtctcgtt gcggccgatc atgaacccac aagatcttgc
     3601 caaggccggt ccatcgattg gcaaggctgc taagcaatac ggaaagtaga cgtgggacag
     3661 gcctactctg tcctgcgccg attttgcgag cggacagagc agcctctcac ccatttccat
     3721 tccgttgccg ctaccatccg ggccgtatac cataccagac gcaatgctct tttctgacct
     3781 cgcactcgcg agacgtttgg agcgtgcgga agggcacgcg tgcgttcaat tcgcggaagc
     3841 acggcgtcgt ctgttccccg acagcggggc cgagtggata gaatgcgccg gggcgtacgc
     3901 aatcttcgat ggcatcgact ctcctgtcac tcagagtttc ggactcggca tattcgagga
     3961 gctgagcgcg gactcgctgg atgtgatcga gcgtttcttt gtgagccacg gggcgccggt
     4021 gcttcatgag gtgagtcctc tcgcgggggt cgccgcgctg ggcttgctct gtgcgcgcaa
     4081 ctacaggccg atcgaaatca gcaacgtctt gtatcagcct gttgagaagc ccgcggcgga
     4141 ccactcgggc aatatcaccg tgcgggtaat cggtccccaa gaagctcgac tctgggcgga
     4201 cataggcgcg aggggctggg catacgaaca tccggaactc ctcgactttc ttctgcaact
     4261 cgcccctatt tccgcggcgc gccggcatag tgtgtgcttc ctcggcgagg tggatggcaa
     4321 accgggcgcc gctggcgtgc tgtgcatgca taacggagtt gcgctgtttg gcggctcggc
     4381 taccattccg gagctgcggc gtcgcggtct gcaagcggcg ctccttcatg ggcgcatgcg
     4441 ctacgccttt gaccatggct gcgatttggc gatgatggtc gccgcggctg gcagcgattc
     4501 ccagcgcaac gccgagcgta aagggttccg gatcgcctac acacgcacca agtggcggct
     4561 gatcgctgac cgctgatagc ttccagcgaa tcagctaatc cacatcgacc gtcaggcgcg
     4621 gcccattgcg aagtgtctga tagacgacgc aatgtcgctc ggtcagctcc agtaacttct
     4681 ggcgttcctc ccgggaagcg tcggtatcga gagagatgcg aaggcggatc tcgcggaagc
     4741 ccaccggcac gtctttggcg acgccgagag tccctcgaaa gtcgaggtcc ccctcggccc
     4801 taagatgtcc gccgcgcagt tcgagtccta acgaagtcgc cacggctgac aaagtcacac
     4861 cggcgcaggc gaccagcgct tcgaggagca tgtcgccaga gcaggcttcc tggccgctgc
     4921 ccccggtggc cggatgcagg cccgcttcga ccaggccgcc gaaggtctcg acacggcagg
     4981 agacgccttc acccagccag ccttcagccg tgagtgtgac gaccgcggct ttgggatctt
     5041 cccgatatcg ttgcttcaag ggcgcctgga gcgcgcgcaa ttcttccctc ttcatgaccc
     5101 cattctcgct tagtggggcg gctccccatt tcttcttctc gttcttcgtg ccttcgtggt
     5161 gaaatctgaa ccgcgcgcat gagcaagcgg agctgaaagc tgatcgctga cagctgacac
     5221 cgaagcgtga caccataatc gaagggagga agcactggca catgatcgaa ctggcagcgg
     5281 aattgcggac cgtcgtggaa cgagcggcag ttcgcttcag gagcctgcgc gaggacgacg
     5341 tcacccgaga cttgggtgcg ggtcggtggg tgaagaagga aatcctcggc catttgatcg
     5401 actcggccgc caacaaccat cagcggtttg ttcgagcgca gttcacgtgg ccgtttgtct
     5461 ggcccgggta cgatcagcaa acctgggtgc ccctgcacca ctatcgtgat cgtccgtgga
     5521 cggaggtggt ggatctgtgg gtggctttga accgccacgt ggcggcggtc atcgaattcg
     5581 taccggtcga gaagctgcaa acaccttgca cgatcggcga ttatgagccg gcctcgctcg
     5641 aatggtggat gcgcgactac ctccgccatc tgaagcacca tctggagcag ttcgaggtcg
     5701 cctgaaacac cgcggttgtt ttgtggcgca ggccatcgtt ttccgtggcc tgcgacggcg
     5761 gcagacgaca aaaggcgagg tcgttttgtg gacaaaaggc gaggccgttt tgtggcgcag
     5821 gccatcgttt tccgtggcct gcgacggcgg tagacgacaa aaggcgatcg ccggccccac
     5881 aagacataag gtcccgagat ggcaacgata tcagaacacc tttcggaacg taagattgat
     5941 gcgagcgttg cctgtcagcg gatgctgccc gtccttgagc ggagcgacgc cgtgatagac
     6001 gaaacgcgcc ggaccgcccc acaccgcaac gtcgccgttt tcgacgcgta ggcggcgcac
     6061 cggatcggag cggcgtttgc cgccccagag gaatacggca ggcaggccca gcgagacgga
     6121 gacaatcgga gcccaggcat ccttctcgtc acgatcttga tgaaggccga gcttggcccc
     6181 tgcgatgtag cggttgatc
//
LOCUS       DATIFQ010000098         5831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_414725, whole genome shotgun sequence.
ACCESSION   DATIFQ010000098 DATIFQ010000000
VERSION     DATIFQ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414725"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            570..902
                     /locus_tag="VLE22_04400"
     CDS             570..902
                     /locus_tag="VLE22_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSB13675.1"
                     /translation="MRSAKRKKLEAAGWQIGTAAQLLGLSKQETELVELRLALARGLR
                     LRREARHLTQASLAKLIGSSQSRVAKMEAADSSVAFDLLLRSLVATGATRRELARIIQ
                     RPTSSSAA"
     gene            complement(1005..1823)
                     /locus_tag="VLE22_04405"
     CDS             complement(1005..1823)
                     /locus_tag="VLE22_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13676.1"
                     /translation="MRVGIVLGAGSVQAEDAWVVQFGAFGGYVYPPCGGIIWYDDLVF
                     HNATDAPLSVQALETSNGGSTNSVPLVIPPHVTRSGYGSLNSEISSWAPDRRTQLWAV
                     HLDVPRGVTVSSRALALIRTPNPEYCGPDPGPTTLFAGPALPIVRQLVPAGVPQILLS
                     ADLGESEKFGGRRTNVGIYNAGTSTASAFVELRRGCDDRLLDRRLISVPAKTIIQVAG
                     PSFSPDTVPGCTALNTTASVNYAVINADQPTFSYIAVLANQLPPITPFTFSFVR"
     gene            complement(2004..2534)
                     /locus_tag="VLE22_04410"
     CDS             complement(2004..2534)
                     /locus_tag="VLE22_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSB13677.1"
                     /translation="MAKLVFGMNQSLDGYVDHMAFAPSPTLFRHFIEEAQGQAGSVYG
                     RRMYEVMRYWDDDHPEWNAEEHAFAAAWRNQPKWVVSRSLKAVGPNAELVERDLEGAI
                     REIKAERDGEIEVAGPDLAHSLTKLGLIDEYRIYLHPVILGHGKPYFAGPRPPLRFIA
                     NDRIDEAVIRLTYVPA"
     gene            complement(2666..2950)
                     /locus_tag="VLE22_04415"
     CDS             complement(2666..2950)
                     /locus_tag="VLE22_04415"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HSB13678.1"
                     /translation="MSKAKRKRLPKFETEEQERKFWAEHDSVDFVDWASAQRRTFPSL
                     RPTLRTISLRLPESMIRHLKVLANKRDIPYQSLLKQFLAERLEHELSKGA"
     gene            complement(3392..5455)
                     /locus_tag="VLE22_04420"
     CDS             complement(3392..5455)
                     /locus_tag="VLE22_04420"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HSB13679.1"
                     /translation="MKISRRSFLKAAAAIGASLAWVGPARGSRVHWHERRDLYPQGVA
                     SGDPDPHSVILWTRRPFAKGTRQLLTVEVAEDEAFRRVIAHAQAPVSSAADWTARVLI
                     GGLKPARTYWYRFTDTDGNGSRVGRTITAPLPNDPRPVNFAFVSCQDVNEGKLNAYRR
                     MIYEDERAPAAQQLDFVLHLGDFIYEVVEYPDEVKTRYDRTIYEVARIPDDHKVGNFH
                     IPLTVDGYRAIYRAYLADPDLQDARARWPFVAIWDNHEFSWQGWQSIVKAGKFEQPGQ
                     SVKVAANQAWFEYLPARVAPPSGLLERFDPPSVKDVPIKKFDSDGLGVEPNNLTAINS
                     LKGYRALRYGQHLDLIITDQHSYRSADPFSDPSLEKLGGDEFIGMFPEPAMQVLDGGR
                     AFNGGNPPPEIRFNDAHVPNPQQSAPPQTILGTEQKAWFKDKLKSSTAVWKIWANSLG
                     ALDWRADPQNLPAGLTKKSWPPNTFAALGGGDYGTAWAERAEIYHLVRDTKITGFAIV
                     SGDRHSFWAGYATAELPPGKFEPIGLSFVGGSLASPGVMEAYEHRLPKDAPLRPLFLA
                     DKDAGAKPAWTFNMLLKHGVRACFEYAKSFDLKRARSLSNPDLAPHLEFVDLGGHGYA
                     KVQLSADEMRTEYVCIPRPIARSERPDGGPIRYRVLHTAVLWKSGERPQLKTSVLEGD
                     VGLAI"
BASE COUNT         1144 a         1807 c         1782 g         1098 t
ORIGIN      
        1 agggagcttc gaagcctcat caaaagcctt ctcgagaacc ttagtcatga ccgggcaatt
       61 ctaccgtctc gccaccgtat ctcgctaaga gtgccgccga acggtcgggc cttcagcggc
      121 gagccgagcg agcacagcga gcggcccgag gagcggcgag tccgctgcaa cgtcatgtta
      181 ggccgcgccc cggtcagctt gacaatatgt cagtcctgac atattgtgct accggcgtat
      241 ggagaagccg ctggcatggc tacgaggaga tcaagactcc tccgttctct accggtgctc
      301 ggatcgaggc tggatacctc ctgcgaagga ttcagcaggg cgagaagctc tcgcttccac
      361 actctcgacc tatgccagcc atcggccctc gttgccacga gcttcggctt cgggatcgcg
      421 agcacacctg gcgaatcatc taccgggtcg atcccgactg cattctgatc ttggagatct
      481 tcgacaagaa aacaactgcg acgcccaaga gcgtgattga cgattgtcgg cggcggttga
      541 gacattacga cgagcacggg aggtgaccga tgaggagcgc gaagaggaag aaacttgaag
      601 ccgcgggttg gcagatcggt acggccgctc aacttctcgg gctgtccaag caggagaccg
      661 agttggtcga gctcagactt gccctcgctc gaggtctgcg gctgcgacgc gaggcccgcc
      721 acctcaccca ggctagtctc gccaagctga tcggatccag ccagtcccgc gttgccaaga
      781 tggaagccgc cgactccagc gtcgcattcg atctgctcct gcgctccttg gtcgccaccg
      841 gtgcgactcg gcgggaactc gccaggatca tccagcgccc gacctcgtct agcgcggcct
      901 aacagcttgc ggctcagcgg cgagccgagc gagcaaagcg agcggcccga gcgaaagcga
      961 gggcggcgag gccgctgcaa cgccatgttg ggccgaatcg ccgattaccg aacgaacgag
     1021 aacgtaaacg gagttatggg cgggagctga ttcgccagca cggcaatgta gctgaaggtg
     1081 ggttgatcgg cattgataac ggcgtagttg accgatgctg tcgtattcag ggccgtgcaa
     1141 ccggggactg tatcgggaga aaacgacggt ccagcaacct ggatgatcgt cttggccggg
     1201 accgagatca gacggcggtc caggaggcga tcgtcgcagc cgcggcgcag ttccacgaac
     1261 gcggacgccg tgctggttcc agcgttgtaa atccccacgt tcgtccggcg gccgccgaac
     1321 ttttccgact caccgaggtc cgcagaaagc agaatttgag gaactcccgc aggcacgagc
     1381 tgccggacta tcggcaatgc cggccccgcg aacaacgtcg ttggtccggg gtccggaccg
     1441 caatactcgg ggttgggagt cctgatcagc gcgagcgccc ggctgctgac cgtgactcct
     1501 cgtggtacgt cgagatgaac tgcccagagt tgtgttctcc gatccggcgc ccaactactg
     1561 atttccgagt tgagcgaacc gtacccggac ctcgtgacgt gcggaggaat tacgagtgga
     1621 acggaattgg tcgagccacc gttgctcgtc tcgagtgcct gaaccgacaa cggggcgtcg
     1681 gtggcgttat ggaaaaccag atcgtcgtac cagattatcc cgccgcacgg cggatatacg
     1741 tatccgccga acgcgccgaa ttgcacaacc caggcgtcct cagcctgcac cgacccggcg
     1801 ccaagcacga tgccaacgcg cattaggaca aacggcgtca accttagtgc cgtacgctgt
     1861 ctctcacccg ggaagcggcc caacgctccg gttcagcggc ggaccgcgca gcggaccgtc
     1921 cgctgcaacc ggttgttaga ccgcagttgg acctagccaa agaccacatt cctgcggcaa
     1981 tgaccttcct gagagccgcg agattaagca ggaacatagg tcaaccggat cacagcctcg
     2041 tcaatccggt cattagctat aaaacggagc ggcggccggg gtccggcgaa atatggcttg
     2101 ccgtgaccaa ggatgacggg atgcaggtag attcgatatt catcgatcag gccaagcttg
     2161 gttaggctgt gcgccaagtc cgggccagca acttcgatct ccccgtcgcg ttcggccttt
     2221 atctcgcgga tcgcgccctc aagatctcgc tcaacaagct cggcgttggg gccgaccgcc
     2281 ttcaacgagc gcgagacgac ccatttcggc tggttccgcc acgccgccgc gaaggcgtgt
     2341 tcctccgcat tccattcagg atggtcgtcg tcccagtaac gcatgacctc atacattcgg
     2401 cggccgtaca cactgcccgc ctgcccctga gcttcctcga tgaagtggcg aaatagcgtg
     2461 gggcttggcg caaacgccat atggtcgacg tacccgtcca gggactgatt cattccgaac
     2521 acgagcttag ccatgtcctc ttccttttct tgcgcgcggg acgtgtttcc tgcggtctaa
     2581 cggtcgggcg ttcagcggcg agccgagcga gcaaagcgag cggcccgagc gaaagcgagg
     2641 gcggcgagtc cgctgcaacg ccatgttagg cgccctttga tagctcgtgc tccagccgtt
     2701 cagccagaaa ctgtttgagc agcgactgat acgggatatc acgtttgttc gccaggacct
     2761 tgaggtgacg gatcatcgac tcgggcaaac gaagggaaat cgtgcgtaga gtcggacgca
     2821 ggctagggaa ggtcctacgc tgagcagaag cccaatcgac aaaatcgacg gagtcgtgct
     2881 cagcccagaa ctttcgctcc tgctcctccg tctcgaactt cggaagccgc ttacgctttg
     2941 ctttgctcat aggcttggcg ttcccttcga gtcatgtctc gcgccgagat tattcggatg
     3001 agcgacccac gaacggtaaa gctaatgaag agtcgccgat cagagtccga ttgccccagc
     3061 gcttggtagc gagcctcgcc aaccgagtgc tcgcgatcgg gcgatacgac gagaggctcg
     3121 ttgaaaaaga cctcctcggc ctcccagaac gcgacacgat gccgttccca gttcttctca
     3181 agattcccct catcccaatc aaagccggtg cagccctcga gggagtcgcc tgccactcat
     3241 cactgagtat atatcagaga tatacggcgc gcaaccgggc acctaacggg tggcgcgtca
     3301 gcggcgcgcg aagagccgaa ggcgatgagc gagcccgctg cacgcgcgtg ttaggcggct
     3361 ccatcacgcc acccatgcct gccaaggtcc gtcaaatcgc cagtccaacg tcgccttcga
     3421 gcaccgatgt cttcagctgc gggcgctctc cgctcttcca cagtacagcc gtgtgtaata
     3481 cccgatacct gatgggcccc ccgtccggtc tttcgctacg cgcaatgggg cgtgggatgc
     3541 agacatactc ggtgcgcatc tcatcggccg acaattggac cttcgcataa ccgtgccctc
     3601 caagatctac gaactcgagg tgcggagcga gatccgggtt ggacagcgac cgtgcgcgct
     3661 tgaggtcgaa gctctttgca tattcgaagc acgcacggac gccgtgcttc agcagcatgt
     3721 tgaaggtcca ggccggcttc gcccccgcat ctttgtcagc aagaaacaac ggtcgtagcg
     3781 gagcatcctt cggcagtctg tgctcatagg cctccatcac tcccggactg gcgagagacc
     3841 caccaacgaa gctcaacccg atcggttcga atttgccggg cggaagttcc gcagtcgcat
     3901 atccggccca gaagctgtgg cggtcgcccg agacgatggc gaagcctgtg atcttcgtgt
     3961 cgcgtacgag atggtagatc tcagcgcgct ctgcccaagc tgtgccgtaa tcaccgccgc
     4021 ccagggctgc gaacgtgttg ggaggccagg acttcttggt gagacccgcg ggcaggttct
     4081 gcgggtcggc gcgccaatcc agcgcgccca gcgagttcgc ccagattttc cacaccgcgg
     4141 ttgagctctt gagcttgtcc ttgaaccatg ccttctgctc cgtcccgaga atcgtttgcg
     4201 gcggtgcgct ctgttgcggg ttgggcacat gtgcatcgtt gaagcggatt tccggaggag
     4261 ggttgccgcc gttgaaggcg cgcccgccgt cgagaacctg catcgccggc tcaggaaaca
     4321 ttccgatgaa ctcgtctccg ccgagctttt cgagagacgg atcgctgaag ggatccgcgc
     4381 tccgatagct gtgttggtcg gtaatgatca ggtccaggtg ctgaccgtac cggagcgccc
     4441 tatagccctt gagactgttg atcgccgtca ggttgttcgg ttccaccccc agcccatcgc
     4501 tatcgaactt tttgatgggt acgtctttta cagatggtgg atcgaaacgc tcgagtaacc
     4561 cgctcggcgg agcgacacgc gcggggaggt attcgaacca agcctgattt gccgcgacct
     4621 tgacgctctg gccaggctgc tcgaacttcc ccgccttgac gatgctttgc caaccttgcc
     4681 acgagaattc gtggttgtcc cagatcgcga cgaacggcca gcgggcgcga gcgtcctgga
     4741 ggtcgggatc ggcgagatac gccctgtaga tggcccggta gccgtcgacg gtcagcggta
     4801 tgtggaagtt tccgaccttg tggtcgtctg ggatgcgcgc aacctcatag atcgtgcggt
     4861 cgtagcgtgt cttcacctcg tcgggatact ccaccacctc atagatgaaa tcgcccaggt
     4921 gcagaacgaa gtcgagctgt tgggcggcgg gagcgcgttc atcttcgtag atcatccggc
     4981 gataggcgtt gagcttgccc tcattgacat cctgacagct cacgaatgcg aagttcactg
     5041 ggcgcggatc gtttggcaac ggtgcagtga tcgtgcggcc gacccggctg ccgtttccgt
     5101 cggtgtcggt aaaccgatac cagtaagtgc gcgccggctt gagtccgcca atcagaacgc
     5161 gcgcggtcca gtccgcggcg cttgagaccg gtgcctgcgc gtgcgcgatg actcggcgga
     5221 atgcctcatc ctcggctacc tcgacggtca ggagctggcg tgtgcccttg gcaaacggac
     5281 gcctcgtcca caggatgacg ctgtgcggat cgggatcgcc cgaggcgacc ccttggggat
     5341 agagatcacg ccgctcatgc cagtggacac gcgaacctcg agccggtccg acccatgcga
     5401 gcgaagcacc gattgctgct gcagctttga gaaaggatcg gcggctaatc ttcatcgtct
     5461 cactccgtcc gggctgtagt tggcgtcacc tcagggttgg acacgaggcc aatcgtcagc
     5521 gcaaggtgaa cagccgtcac cacgagaagc cgcttcgtca ctctcatcgg gtagggccta
     5581 acggtcgggc gttcagcggc gagccgagcg agcaacgcga gcgacccgag cgaagcgagg
     5641 gacggcgagt ccgctgcacg cgcatgttag gcggcggctc agaaagcaca ccagactcca
     5701 tccggtgtgc tcaacactct gccattcacc aagtcaacag ctacccgaag cctcaccgcc
     5761 gtccgccgct ccgggccagc tcgcgcaggg cctccgccag gagcgcgttg aaggcctcgg
     5821 gctgctcgag c
//
LOCUS       DATIFQ010000099         4046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1755587, whole genome shotgun sequence.
ACCESSION   DATIFQ010000099 DATIFQ010000000
VERSION     DATIFQ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755587"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /locus_tag="VLE22_04425"
     CDS             <1..1787
                     /locus_tag="VLE22_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HSB13680.1"
                     /translation="DRGWWRYVSVADVARIGLVNLSGSALSAAIIVAVVPPGFPRSVY
                     LLDLILCVLASAGVRVMVRVIAEAAAQNRHERGAKRTLIYGAGEAGITLLREIRSNPR
                     LPYRVCGFVDDHPEKAGVRIQGRPVLGSGADLEALAREHNVEEVLIAVPSATGEQMLG
                     ILEKCRAGNVRCKTVPGLAEVVEGKGLAGQIRDVAMEDLLGRSPVRLEEEQIREKLEG
                     KVVLVTGAAGSIGSELCRQIARFRPAAVVGFEVGETPLFHLESEMRQLFPNVTFHAEI
                     GSVQNTRRLEEVLRLYEPSILYHAAAYKHVPMMEAHVFEAVENNVFGTYNAARLAAEC
                     GLTDFVMISTDKAVRPTSVMGATKRVAELVVNSLQNGGTKFASVRFGNVLGSNGSVIP
                     LFKKQIADGGPVTVTHPDMQRYFMTIPEAAQLVLQASTMGRGGEIFVLNMGKPVKILD
                     LARNLILLSGLRPSEDVKIEFTGVRPGEKLFEELHTCDESTAPTRHEKISIYSGACVS
                     LEDMYPRLGLLKQICAEREVDSLLLWLKDLIPEYNPSGHVLRRALSSSVNTRKEVGVR
                     PELTLHATAGWNGTYVELEHAAAHAPIS"
     gene            1821..3200
                     /locus_tag="VLE22_04430"
     CDS             1821..3200
                     /locus_tag="VLE22_04430"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSB13681.1"
                     /translation="MGYLVLLGLVFAYAVYLDGGATRWSWELCLPVIGLTGLIYWLRT
                     RRTELAPPLEGWLLRAALLLPAYAAVQAIPLPLPVLQILSPARAELQVALSKAGAAGD
                     FASLSVVPPVTLRHGVTLAGLLVVLLLVRELAWRWPERRWTLASPFVFVAALEAALGI
                     AQAAEGDGAHGTYVNHNHFAGLLEMALPFAVVYAVVMTRRVLRSEKNEVCARRLRPVV
                     KPVVGACVALSAAVLIVAGVVASQSRMGFVSALTGLAAAMVLLTIWCKQSTNHRRNAL
                     AVALAVALAGAGLALLPSEALVTRFGHLAQQDLSTDTRTKIWNETLALIAAYPVFGCG
                     FGGYESAFLKFKSAAPMYTVDYAHNDYLQGVAELGVAGFLLAACFLVAVVRTLLRGRL
                     SSDREERCFIIACLGAMTAILLHSITDFNLYMPANAMLLAWICGAGLGSLRYSPGGAA
                     RIVDVAPAR"
     gene            3260..3865
                     /locus_tag="VLE22_04435"
     CDS             3260..3865
                     /locus_tag="VLE22_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13682.1"
                     /translation="MRTSNYVLLFVVIVLVGSLQAADHVGTVTAGGPLMLRGQLVPHE
                     AAHGMALTSGDEVETIDDPAMIMLNDQGRVTVEPRSLVKIILSGSRTQVSLGRGTIQY
                     GVTAASKLSICASSRLIEPAAPSEGVVTIDGPGKVNVATAQGSLKVDEKTACGYDGQP
                     AKWLTKTKAIVVVGTTAAAGALVGIGKSQTGPPNPTSVSPK"
BASE COUNT          774 a         1133 c         1342 g          797 t
ORIGIN      
        1 tggaccgggg gtggtggcga tacgtgtccg tcgcggatgt ggcacggatc ggtctggtga
       61 acctctcagg ctcggcgctg agcgcagcaa tcatcgttgc ggttgtgcct cctgggtttc
      121 cgaggtcagt ctatctgctc gacttgatcc tttgcgtgct cgcctccgcg ggggtgcgtg
      181 tgatggtgcg ggtgatcgcc gaggccgcgg cccaaaaccg gcacgaacgt ggggcgaagc
      241 gaacgctgat ctacggcgcg ggggaggcgg gcatcacgct gttgagggag atcaggagca
      301 atccccggct gccatacaga gtgtgcggat ttgtggacga tcatcccgaa aaagccggcg
      361 tccgcatcca agggagaccg gtgcttggtt cgggcgccga cctggaggcg ctggcgcggg
      421 agcacaacgt cgaagaggtc ctgattgcgg tgccttcggc aaccggcgag cagatgctcg
      481 ggatcctcga gaagtgccgc gcagggaacg tgcggtgcaa gacggtgcct gggctcgccg
      541 aggtggtgga agggaaggga ctcgcaggcc agatccggga cgtagcaatg gaagacctgc
      601 ttggccggag ccccgtacgc ctggaagagg agcagattcg cgagaagctc gaaggcaagg
      661 tggtgctggt gacgggggcg gcgggatcta tcggaagcga gttgtgccgg cagatcgcac
      721 gattccggcc ggcagccgtc gtcgggttcg aagtcggcga gaccccgttg ttccacctcg
      781 aaagcgagat gcggcagttg ttcccgaacg tgacgttcca tgcggagatc gggagcgtcc
      841 aaaacacacg ccggctggag gaagtcctcc ggctgtacga gccgtccatc ctgtaccatg
      901 cggccgcgta caagcacgtg ccgatgatgg aggcgcacgt gttcgaagcc gtcgagaaca
      961 acgtcttcgg cacgtacaac gccgcacgac tggcggcgga gtgcggactg acggacttcg
     1021 tgatgatctc aacggataag gccgttcgcc ccaccagcgt catgggcgcc acaaaacggg
     1081 ttgccgaact cgtggttaat tcccttcaga acggcggaac gaagttcgcg tccgtgcggt
     1141 tcggaaacgt gctcgggagc aacgggagcg tcatcccgct cttcaagaag cagatcgccg
     1201 acggcgggcc ggtgacggtg acgcacccgg acatgcagcg ctacttcatg acgataccgg
     1261 aggcggccca acttgtgctc caggcatcca cgatggggcg gggcggcgag atcttcgtcc
     1321 tgaacatggg caagccggtg aaaatcttag atcttgcccg taacctgatc cttctgtccg
     1381 gcctgaggcc gagcgaagac gtcaagatcg agttcacggg tgtgcgtccg ggggagaagc
     1441 tctttgagga attgcacacc tgtgacgaat cgaccgcacc gacgcggcac gaaaagatca
     1501 gtatctactc cggcgcctgc gtgtccctcg aagatatgta cccgcgccta ggcctgctga
     1561 aacagatctg cgcggagcgg gaagtcgact cactccttct ttggttgaag gacttaattc
     1621 cagagtacaa tccgagcggc cacgtgttgc gccgcgctct ctcgagttcc gtgaacacca
     1681 ggaaagaagt cggagtgcgg ccggagctga cgcttcacgc caccgctggc tggaacggca
     1741 cgtatgtgga gctcgaacac gccgcggctc acgcacctat aagttgaaag cccgatctga
     1801 gaaccagacg agacagtaac gtgggttact tagtactgct aggcttggtc ttcgcatacg
     1861 ccgtctatct ggacggcggt gcgactcgat ggagctggga gttgtgtctc ccagtcatcg
     1921 ggctgacggg ccttatctac tggctccgca cacgccggac cgaacttgcg cctccactcg
     1981 agggctggct cctgcgagct gcgctcctgt tgccagctta cgcggcggtc caggccattc
     2041 cgttgccgct ccctgtactc caaatacttt cgcctgcccg agcggaactc caggtcgcac
     2101 tgagcaaagc cggtgcagca ggggacttcg cgtctctcag cgtggtcccg ccggtgactt
     2161 tgaggcatgg tgtcacgctc gcaggcttgc tagtcgtgct tttgctcgtt cgggagctcg
     2221 cctggcggtg gccggaacgg cgctggacac tggcatcccc attcgtgttc gtagccgccc
     2281 ttgaggcggc gctgggcatt gcacaggccg cggaagggga cggtgcgcac ggcacgtatg
     2341 tgaaccataa ccacttcgca ggtttactcg agatggcctt gccgttcgcg gtagtgtacg
     2401 cggtcgtgat gacgcgacgg gtcctccgga gtgagaagaa cgaggtttgc gcgcggcgcc
     2461 tgcggccggt ggtgaagccg gtagtggggg cgtgtgttgc actttcggct gccgtcctga
     2521 tcgtggccgg ggtggtggct tcacagtcac ggatgggttt cgtgtcggcg cttaccggct
     2581 tggccgcggc gatggtgctt ttgacaatct ggtgcaagca gtcgaccaat caccggcgga
     2641 acgcactcgc ggtggctctg gcggtggctc tggctggggc agggctggcg cttctgccat
     2701 cagaggcact ggttacaagg ttcggacacc ttgcgcagca ggatctatcg accgatacaa
     2761 ggacgaagat atggaacgaa acgttggcgc tgattgcggc ctatcccgtc ttcggctgtg
     2821 ggttcggtgg gtatgaatcc gcgtttctga agttcaagtc ggccgccccg atgtacacgg
     2881 tagactatgc gcacaacgac tacttgcagg gagtagccga actgggagtg gcgggctttt
     2941 tgctggcagc ctgcttcctg gtcgccgtcg ttcgcacact gctacgcggc cggctgtcat
     3001 ccgatcggga agaacgctgc ttcatcatag cgtgccttgg cgcgatgacc gcgatcttac
     3061 tgcacagcat cacggacttc aatctctata tgccggcgaa tgcgatgctt ctggcatgga
     3121 tttgcggggc tggacttggg tcgttgcggt atagcccagg aggagccgcg cgaatcgttg
     3181 atgtagctcc ggctcgataa gatgccggtg ttcgatgttc ttaggctaac tcaataaggg
     3241 acttttcaag gaggtcaacg tgcgtacttc aaactatgtt ctgttgttcg tagtgatcgt
     3301 tttggttggt tcgcttcagg cggctgacca cgtcggaaca gtgacagcgg ggggcccgct
     3361 catgttgcga ggccaactgg tgccacatga ggctgcccac ggaatggcgc tcacgagcgg
     3421 tgatgaggtg gagactattg acgatcccgc aatgatcatg ctgaatgatc agggacgggt
     3481 gacagtggaa ccacgaagtc tagtgaagat tatcctctcc ggaagtcgaa ctcaggtgag
     3541 cctcggacgg ggcacgatcc agtatggcgt gaccgccgct tcgaagctga gcatctgtgc
     3601 gagctcacgg ttgatcgaac ccgcagcgcc gtccgaaggt gtagttacga tcgatggccc
     3661 cggtaaggtc aatgtcgcca ccgctcaggg aagcctgaag gtggacgaga agacggcgtg
     3721 cggctacgac ggacaacccg cgaaatggct gacaaagacc aaagcgatcg tagttgtggg
     3781 gacaaccgct gccgcgggcg ctcttgtggg gatcgggaag agccagaccg gaccacccaa
     3841 cccgacgagc gtcagtccca agtagcgctg acgttcgagc atccgacact cgcccactgt
     3901 cgctgcatat ctccagctcc agccaaacag gtatgccgat gctggaaatg tcaagatcga
     3961 atccaccagg cggtatatat cgtgtcatcg cgcacacctt tgggcgcttt atctcaggca
     4021 ccactgcaca aagcctaggt cacatc
//
LOCUS       DATIFQ010000100         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1755761, whole genome shotgun sequence.
ACCESSION   DATIFQ010000100 DATIFQ010000000
VERSION     DATIFQ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755761"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VLE22_04440"
     CDS             complement(<1..1340)
                     /locus_tag="VLE22_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HSB13683.1"
                     /translation="MALEPVAPVAAATRTEWVCPMHPEIVRDAPGSCPKCGMTLEPRT
                     AAAGEEENAELVDMRRRFRVSVALTIPLVVVAMGEHLPGHPLARISATESMGWLQLVL
                     ATPVVLWGGWPFFVRGWQSILNRSLNMFTLIGLGVGVAYGYSLVAVLFPGIFPPSFRD
                     ASGMVAVYFEAAAVIVTLVLLGQVLELKARSQTGAAIKALLGLAPNTARRLGDNGSDE
                     DVPLDQVHVGDRLRVRPGEKVPVDGVVLEGTSAVDEAMVTGEPIPVEKKPGDHVVGAT
                     VNGTGSFVLRAERVGADTLLARIVRMVAEAQRSRAPIQKLADQVAGWFVPAVVSIAVA
                     TAIVWGLVGPEPRMGHALVNAVAVLIIACPCALGLATPMSIMVATGKGATAGVLFRNA
                     EAIEILRKVDTLVIDKTGTLTEGKPKLVTVDPAKGWDEKGLLRLAASLERGSEHP"
     gene            complement(1477..1974)
                     /locus_tag="VLE22_04445"
     CDS             complement(1477..1974)
                     /locus_tag="VLE22_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HSB13684.1"
                     /translation="MMWRILVASAIAMSLVSATGARAHDDKHHEKKEERQADGHAAAI
                     GKPGDPGKVTRSVEVEMIDTMRFKPDSIRVKQGETIRFIVRNTGKVKHEMVLGTIKEL
                     KEHAEMMRKFPEMKHADPNQVSVEPGMTGELVWQFTKAGTFDFACLVPGHFESGMVGK
                     VLVGH"
BASE COUNT          429 a          843 c          723 g          463 t
ORIGIN      
        1 ggatgctcgc tcccacgctc cagactggcg gccagccgca aaagaccctt ttcgtcccat
       61 cccttggccg ggtccaccgt gaccagcttc ggtttccctt cggtgagcgt cccggtcttg
      121 tcgatcacga gggtgtccac cttgcgcagg atctcgatcg cctcggcgtt ccggaacagg
      181 acgccggcgg ttgcaccctt tcccgttgcg accatgatcg acatcggggt cgcaagcccg
      241 agcgcgcagg ggcaggcgat gatgagcacg gcgaccgcgt tgaccagcgc gtgtcccatc
      301 cgcggctccg ggccgacgag tccccagacg atggccgtgg cgacggcgat cgagaccacc
      361 gccggaacga accagccggc cacctggtcc gccagcttct ggatcggcgc gcggctgcgc
      421 tgcgcctccg cgaccatccg gacgatccgt gccagcaatg tgtcggcgcc cacccgctcg
      481 gcccgcagga cgaacgagcc cgtcccgttg accgtggcgc cgaccacgtg atcacccggc
      541 ttcttctcca ctggaatggg ctcccccgtg accatcgcct cgtccaccgc gctggtccct
      601 tcgaggacga cgccgtccac cggcaccttc tccccgggtc gaacgcgcag ccggtcccca
      661 acgtgaacct gatcgagggg gacatcctca tcggagccgt tgtccccgag ccgccgcgcg
      721 gtattcggcg caagccccag aagcgccttg atcgccgccc cggtctggct gcgcgccttc
      781 agttccagga cctgcccgag cagcacaagg gttacgatga cggccgctgc ctcgaagtag
      841 acggcgacca tgcccgacgc atcgcggaac gaggggggga agatacccgg gaatagtacg
      901 gccaccaggc tgtaaccgta cgccacgccg acgcccagcc cgatcagcgt gaacatgttg
      961 aggctccggt tcaggatcga ctgccagccg cgcacgaaga agggccagcc tccccacagg
     1021 acgacgggag tggcaaggac aagttgcagc cagcccatcg actccgtggc ggagatccgc
     1081 gcaagtggat gccccggcag atgctccccc atggccacca ccacaagggg gatcgtgaga
     1141 gcgacgctga cccggaagcg tcgcctcatg tcgacgagtt cggcgttctc ctcctcgccg
     1201 gccgcggccg tccgtggctc cagcgtcatg ccgcactttg gacagctgcc aggcgcatcg
     1261 cggacaattt ccggatgcat ggggcagacc cattccgtcc gggtggcggc cgcgacgggg
     1321 gcgaccggtt cgagcgccat gccgcacttc gggcagtttc ctgggccgga ctgccgtacc
     1381 tcggggtgca tcgggcaggt gaacacccct gcggttgtct cagcaggtgg cgcgctttcc
     1441 cttgagggca ttcccatcgc cgcttccgtc tgcgcctcag tggccgacca gtactttacc
     1501 gaccatgccc gactcgaaat ggccgggcac caggcaggcg aaatcgaacg tacccgcctt
     1561 ggtgaattgc cagaccagtt cgccggtcat gccgggttcg acgctgacct ggttgggatc
     1621 ggcgtgtttc atctccggga atttccgcat catctcggca tgctccttca gttccttgat
     1681 cgttccaagc accatctcgt gcttcacctt gccggtgttc cggacaatga accggatggt
     1741 ctcgccctgt ttcacacgga tgctgtcggg cttgaagcgc atcgtgtcga tcatttccac
     1801 ttcgacgctg cgggtgacct tgccgggatc tcccggcttg ccgatagccg ccgcgtggcc
     1861 gtcagcctgc cgttcttcct tcttctcatg atgcttgtcg tcgtgggccc gcgcaccggt
     1921 ggcggaaacg aggctcatgg caatcgcaga agcgacgagg attcgccaca tcatggtttg
     1981 tccccttcct tggtcagttg tgcccttgga catcgtcctt gcgcacgtcc agcaggctga
     2041 tggtctattt agaagccttt cagttggata ccgtttcctc actccaagca ccagttacgt
     2101 cactcggcgg agcggttcgg tacagccatg ccctctgcca tgccctctac catctgccgg
     2161 aaaacatttc ccttgacacg tcgcatccaa taaatatact acataaccca tagggtatat
     2221 atttatttag acggctccat ccgacgcgaa gtaccctaca aaatctaccg ctttaaggag
     2281 gtaccatgaa acgcctgctc ctcttcgtcg ccgtggcgct gctcgccgtc cagccggctt
     2341 acgccgaaac ggtaaagatc aacctccact cctgctgcgt cacggaggac gccacgacga
     2401 aggagatcct cccggcgttc aggaagtact acaaggagaa gttcggaaag gaggtgga
//
LOCUS       DATIFQ010000101         3030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_224425, whole genome shotgun sequence.
ACCESSION   DATIFQ010000101 DATIFQ010000000
VERSION     DATIFQ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224425"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..667
                     /locus_tag="VLE22_04450"
     CDS             125..667
                     /locus_tag="VLE22_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSB13685.1"
                     /translation="MTRYQQADSAAASALVKRLSPRLFRFFASQADSPADADDMLQDL
                     WLRLHRVRHTYRAGEPLLPWAYAIAHRVRIDSYRRRRRISREIAVDVLPEPAAHEEKQ
                     SDLPAFDELLAALPESQREVLTMLKVDGFTVEEIARATSSTAGAVKQKAHRAYERLRN
                     LLQEMAARQVRKGGTPQRGA"
     gene            740..1594
                     /locus_tag="VLE22_04455"
     CDS             740..1594
                     /locus_tag="VLE22_04455"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HSB13686.1"
                     /translation="MEGAEDGARAEAQGGPKVTCRDIDGFIILHVSGAAIPPEAAAHI
                     AGCERCRQLADAIGETQRTALPSPESLTRIENRILADLKPVKPLAPTSVLFFALMFFL
                     VVVAAIGSAALGTSGWRVLSALQRIAVFTALAATAGLLAFSAVRQIVPGSRLLLSPYW
                     PEIAVLGVMTGILAALFHPREESAFVSRGLKCLGIGLEYAIPTALLAWLLLRRGAVLN
                     PVLTGATAGMLAGLSGLTVLEIHCPNLNAYHILAWHIGSVLASVLGGVAIGLMAAYSG
                     GRRTRRIP"
     gene            complement(1931..2107)
                     /locus_tag="VLE22_04460"
     CDS             complement(1931..2107)
                     /locus_tag="VLE22_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13687.1"
                     /translation="MIPAGLDDLIPMEVLVGTLWTLIVVMRALLLVGGVCFLVGACFL
                     VAMANKRGSWHATG"
     gene            complement(2154..>3030)
                     /locus_tag="VLE22_04465"
     CDS             complement(2154..>3030)
                     /locus_tag="VLE22_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HSB13688.1"
                     /translation="SAPAKLVRRFEGVNEWPTYSPDGTRLSYVSARGTVARAQNRRDT
                     LCILDLKTARTKDFVTDFRQIYNPLWLSRNSGLIVPAQKRSEDIGLYLFDIETGRFTL
                     IVQFRYGDLLHHDVAPDGKTLYYARRDKEKDVTEFVARDLDTGTERLLHTVTPSEWIR
                     TALSPDGTQLAYINRAEDRVIRVIPTSGGEPRDAVRYRHIGNHLVQFTWSADGKYFLV
                     PRIDNPPPPASGWSLWRFRVSDGQGEKILSNCGRATKTTVHPDGQRIAFASPGAGPQN
                     AELWVVEHYLPENGK"
BASE COUNT          573 a          892 c          965 g          600 t
ORIGIN      
        1 ccaccgcttt tcttgagaat cacttcgttc cgattcggag gccgtcggaa actaccggat
       61 ggtaagatgt taggattccg ccgtggaacc cgagggatct tgcgagcctg acctggagca
      121 gctgatgacg cggtaccagc aagcggattc ggcggccgcg tctgccctcg ttaagcgcct
      181 cagcccccgc ctgtttcgct tctttgccag ccaagcggat agcccggccg acgccgacga
      241 tatgctccag gacctgtggc tgcgcctgca ccgcgtgcga cacacttacc gcgcgggcga
      301 gccgctgctt ccctgggcgt acgcgattgc gcatcgggtg cgaatcgaca gttatcgccg
      361 gcgccggcgg atctcgcggg aaattgcggt cgatgtgcta ccggagcctg ccgcccacga
      421 ggagaaacaa agcgacctgc cggccttcga cgaacttctg gcggccttgc cggaaagcca
      481 gcgagaggtg ctgacgatgc tcaaggtgga tggatttacc gtcgaagaga tcgctcgggc
      541 cacgtcttcc acggccggcg cggtcaaaca gaaagctcat cgggcgtatg agcgactgcg
      601 caacttacta caagagatgg cggcccggca ggtacggaaa gggggtacac cccaacgtgg
      661 agcatagtac cccaagttcc aatcatggta acaagcgtta ctcggcgcac agtatcagcg
      721 aatcattaga ggagagagga tggaaggtgc tgaagacggt gctcgggcgg aggctcaggg
      781 aggaccaaag gtgacgtgcc gtgacatcga cggcttcatt atcttgcacg tctcgggtgc
      841 tgccatccca ccggaggctg ctgcccacat tgctggatgt gagcgttgcc ggcagttggc
      901 cgatgcaatt ggggagactc agcggactgc cctaccgtcg ccggagagtt tgacacggat
      961 cgagaaccgg atactggcgg acttgaaacc ggtgaagccg ctggccccta ctagtgtgct
     1021 cttcttcgcc ttgatgttct ttctcgtggt tgtagcggcg attggtagtg cggcgcttgg
     1081 gaccagcggc tggcgggtgc tcagtgcgct gcaaaggatt gccgttttca ctgctctggc
     1141 ggcgactgcc ggtctgctcg ccttctcggc ggtccggcag atcgtgcccg gaagccggct
     1201 cttgctgtct ccctactggc cggaaattgc tgtgttgggc gtcatgaccg gaatccttgc
     1261 agccctgttt catccgcgcg aggaatccgc gttcgtctcg aggggcctga agtgcctcgg
     1321 aatcggactc gagtacgcca ttccaaccgc gctcctggcg tggctgctgc tgcgccgtgg
     1381 ggcagtccta aaccccgtgc tgaccggggc aacggcgggc atgttagccg gtttgagtgg
     1441 gcttaccgtg ctggagatac actgcccgaa tttgaatgcg taccacatcc tcgcctggca
     1501 cataggatct gtgttggcga gtgtcctggg tggggtggct attggtctta tggcggcgta
     1561 ttccggcggg aggcgaaccc gccggattcc ttgagggcag tcccgttgct tttgacgcaa
     1621 taggagtgcc cgctcccagg agccggtaca gcccggccag ctccagatac gggccacccg
     1681 acccccgcgt cgcatagaag atgttgagga aagcctttgc aggaaggtca gtgcggagcg
     1741 aagatcgcga acctgcgccc gcgccaagcg aaggacgctg tgtgctcatc gaaaccgcca
     1801 gtattgcgcc gcgggggaac ggacacttgg gattctcatt cctgacaccg taacactact
     1861 gcgaacggcg tttcagccag acttccaagt cgccggccac cggcagaggc ccggttcgtg
     1921 gtcttacctc ttagccagtg gcgtgccagc taccgcgctt gttggccatc gccacgagga
     1981 aacaggcccc caccaggaaa cagacccctc ccactaacag gagtgcccgc atcacaacaa
     2041 tcagggtcca cagggtgccg accagaactt ccatcgggat cagatcatca agaccggccg
     2101 gaatcatcac atcgctccct gctaacaagt agtgagacgg cttctgccat cagtcacttg
     2161 ccgttctcag gcaagtaatg ctccacgacc cagagctcgg cgttctgggg tccggcgccg
     2221 ggcgaagcga aagcaatgcg ctggccatcg ggatggaccg tcgttttcgt ggcgcgcccg
     2281 cagttggaca gaatcttctc cccctggccg tctgagacgc ggaaccgcca aaggctccaa
     2341 ccagacgccg ggggcggcgg gttatcaatc ctaggcacca ggaaatactt gccgtccgca
     2401 gaccatgtga actggacgag gtggttaccg atgtgacgat agcggaccgc gtcgcgcggt
     2461 tcgcccccgg aagtcggtat aacccggatg actcggtctt ccgcgcggtt tatgtaggcc
     2521 agttgagtgc cgtcgggaga cagggcggtt ctgatccact ccgagggcgt caccgtgtgg
     2581 agaagccgtt ctgtgcccgt atcgagatcg cgagccacaa actcggtgac gtccttttcc
     2641 ttgtccctgc gggcatagta gagggtcttg ccgtcagggg cgacgtcgtg gtggaggagg
     2701 tcgccatacc ggaactgcac tatcagagtg aaacggccag tctcaatgtc gaacaggtag
     2761 agcccgatat cctcgctcct cttttgtgca ggcacgatca agccgctgtt gcgggataac
     2821 cagagcgggt tgtagatctg ccggaaatcg gttacgaagt ctttcgtcct agcggtcttc
     2881 aggtccaaaa tgcatagagt gtcccgccgg ttttgggccc gggcgacggt tcccctggcg
     2941 gagacgtagg agagtctcgt gccgtcgggg gaataggttg gccattcgtt cactccttcg
     3001 aagcggcgga ccagtttcgc gggagcagag
//
LOCUS       DATIFQ010000102        10756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_990740, whole genome shotgun sequence.
ACCESSION   DATIFQ010000102 DATIFQ010000000
VERSION     DATIFQ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990740"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VLE22_04470"
     CDS             <1..943
                     /locus_tag="VLE22_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB13689.1"
                     /translation="YGQFLAAAVILLLAGVNYVGVRLSGGIQVSVTIVKVVLLAGIIV
                     VGLYWGRGTMGNYGSSAPAIGGVAGFFAALVAALWAYDGWNNLSMVASEITRPRRNLP
                     IALIAGTIAVMGIYLLANLAYFYVLPAAAVASSDRVAAEMMRRIAGSGGAGAVSIAAM
                     VSIFAALNGSILSGSRVPYAMARDGLFFSPLGRVHPKHRTPGTSILVLSVWAALLVFS
                     GRYEELFTYVIFASWIMYGMTAVSVIVLRKKRPHLSRPYRTIGYPTVPVLFVLGAVCV
                     VVSTLLNSPRESVMGLVLILFGLPFYFRWKRRKVDVA"
     gene            complement(1419..3755)
                     /locus_tag="VLE22_04475"
     CDS             complement(1419..3755)
                     /locus_tag="VLE22_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HSB13690.1"
                     /translation="MKTRLLLLAAAVLVAPLSPAWGVFPGSRDLDAAIERAIQEDQIP
                     GAVLLVSHHGKIVHRKAYGFRSLTPRREKMTLDTIFDAASLTKVVATTSAVMKLFEDG
                     KLRLNDRVTVYLPEFQGGKSNITVRDLLTHFSGLRPDLDLKPEWKGYETGIRLALADT
                     PVADAGTRFIYSDINFILLGEIVRRVSGNPLPEYAGEHVFRPLGMKDTMFQPPARLRR
                     RIAPTETVPGHAGPLRGVVHDPTSRYMGGIAGHAGLFTTADDLSRFAGMMLGMGQPES
                     RRIFSPLVVRKFTEPQSPPSQPILRGLGWDIDSPFSGNRGELFPIGSYGHTGFTGTSL
                     WIDPATQSYVILLANSVHPRLRPPITSLRCRVATVVAASLDMKVPAISITGYNEAQAG
                     LRRVVARNGQVLTGLDVLAAQNFAPLAGKRVGLITNHTGLDRNGRRNVDLMLEAGIAV
                     KALFSPEHGIAGREDTPDVGHATDPATGLPIWSLYSGQNRRPTEEMLRDLDVLVFDVQ
                     DIGVRFYTYVSTMGYAMEEAARGKLPFYVLDRPNPLTGIYVEGPVLDKELESFIGYFP
                     LPLRHGMTAGELARLFNEEKSIGADLHVVAMQGWQRGDWFDSTGLVWVDPSPNMRSFN
                     AALLYPGVALLEYARNYSVGRGTDAPFEQVGADWINGRELAAELNSKFIPGVRFYPTR
                     FRPASSNFEGALIEGVRFVITDREAFRGERLGLEIAAALGKLYPGKILLEANRGLIGS
                     VETIAALETGEEPREIQWKEEQKLREFVQLRKKYLLYQ"
     gene            complement(3752..4051)
                     /locus_tag="VLE22_04480"
     CDS             complement(3752..4051)
                     /locus_tag="VLE22_04480"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HSB13691.1"
                     /translation="MANVSRLSDSEIQAALKGLPEWKVVNGKLHREYRFPDFVHAFGF
                     MATAAVAIEAMGHHPEWFNVWNRVTVDLMTHDAAGITSKDIALAEKLESIAKKLQ"
     gene            4175..5392
                     /gene="amrB"
                     /locus_tag="VLE22_04485"
     CDS             4175..5392
                     /gene="amrB"
                     /locus_tag="VLE22_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HSB13692.1"
                     /translation="MPSRLPRLRTNLDFMPSPVADRPGLLIRDSYQYSDVTLIVPPIL
                     VQCLPFFDGEQSDLDLRQALVQLTGEIRIGDVQDQLVEALSKAGFLENEVYFRLRDEK
                     HREFAEAGVREATHQGSAYPEDPGELRQVLLRYLEDGKPAPAPGTLIGIAAPHVSPEG
                     GWPTYQAAYHTLREEYRDRTFVILGTSHYGAPDRFGLTRKPFVTPLGQARTDTVLVDR
                     IAAEAPESVLMEDYCHAVEHSIEFQVVFLQYLFGPDVCILPILCGSFAKSIYEGGRPE
                     DTEEVRRFLGVLGDIAAREGDRLFWVLGIDLAHMGRRYGDRFEAQAGHGEMLTVEQRD
                     KERIERVKEGDAAGFWDRVQHQQDDLKWCGSSPLYTLLKAVPQARADLLRYQQWNIDE
                     QSVVSFAALAFRR"
     gene            5463..5690
                     /locus_tag="VLE22_04490"
     CDS             5463..5690
                     /locus_tag="VLE22_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13693.1"
                     /translation="MKKRDLTIVWIALIGIVFWLVTVRPAPIRKPLRRAQLFIERIVR
                     GKEPSQSKAGKARAGAKRKTPEKSSAREPVP"
     gene            5812..6432
                     /locus_tag="VLE22_04495"
     CDS             5812..6432
                     /locus_tag="VLE22_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13694.1"
                     /translation="MLALLAACLSADAAGPQVWNSSEFTFYETGWFRADLLGVVRSRN
                     HLTDAFDNRLGAQVVFPIRPRLSIGGGYLKRWVDPDGRGAHLEDRIFLAPRLLLAYRP
                     IKIESLTSWERNFSVPGLPDFNRYRETIDVEKVRHGASPFFFEQVSFRDYGLAWTRTA
                     VGLRWRLHSGFRFEAGYQFESQKIAGAWAPRHAIRTVIGFRKPRPN"
     gene            complement(6458..8305)
                     /locus_tag="VLE22_04500"
     CDS             complement(6458..8305)
                     /locus_tag="VLE22_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13695.1"
                     /translation="MSSESEPSGDKFPFEAGSRRRGHLTYVPVAPGRLEFAVELRRAL
                     LRERPGIVAVELPVTLEQAYLSAVGRLPEISVVVYGEEDGEDDRAIYVPVEPADPFTE
                     AVRTALEIGAEIVFIEPDFGEKPHVPDRYPDPYSLYSTGLEKYIEAYRLFPQPRSEKL
                     TAQGRGIAWKLQGLNSEAAAFVVLSLNSLDPVLDAMEEPQAQPMSRQRRSGVQVLNPH
                     PDCLAEITVEYPYLQYRYEQFRVEMTSVALADRRRTQFAVLRDAEKSYETATGERVAH
                     WQRRLMARYTRNLALSAGDLTAGLFDITVAARCVVDDNYAWDVWETAGKYPPQKTASD
                     VVTVNLSGEEVWLDTKKIRLRRRLPRPKQRLRPVGLKPRKKEKFPGEWARQIDGSAIC
                     SYPPEDLVVENYGRFLQKKAKSILSEERVRTEPFTTSILDGIDLRETIRNWHESRIYV
                     RQFDKIAGEVGSVVVIFDEDRDDRYPYLTTWLGEHQNESDMAFYSTDPFGNLVGPGVG
                     RAEYGGFLMSLPPLRMFDVWGDPDYDLARNKPERLLLAGLDYSVHRHVVYVAAKPPRS
                     IFRSIASRMNRSILYIPIGQLSPTKLKKIRVVHVLDSHARRDTAKEYIW"
     gene            complement(8289..9116)
                     /locus_tag="VLE22_04505"
     CDS             complement(8289..9116)
                     /locus_tag="VLE22_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSB13696.1"
                     /translation="MVVEIEGVALHLAHPDELPGRWVGQDEVMRQLLAAWLVVDPQDV
                     PMNPRLVGKPGVGKTTLAYAAGRRLGRDVYIMQATVDTRPEDLLVTPVIEGEGKLRYV
                     ASPLVTAMLRGGVVILDEGNRMSEKSWASLAPLLDTRRYVESIVAGIKIKANPNFRFV
                     ATLNDDASTFDLPEYIHSRLQPQILIDFPERDEEYQILRENLPFGEERILQYVTDFLQ
                     QAHSADERYTVRDGINIARFAMKLTGLSANGFHERQAIETAILQTLGEEAMRYVERI"
     gene            complement(9180..9521)
                     /locus_tag="VLE22_04510"
     CDS             complement(9180..9521)
                     /locus_tag="VLE22_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HSB13697.1"
                     /translation="MIRVKRIYDPPAKGDGLRILVDRVWPRGMSKEKAAVDLWLKHVA
                     PSTELRKWFGHEPERWSEFKRRYQIELSKHADTLNEIRSRGRKGAITLLFAAKDTAHN
                     NAVALLEYLAK"
     gene            9614..10171
                     /locus_tag="VLE22_04515"
     CDS             9614..10171
                     /locus_tag="VLE22_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSB13698.1"
                     /translation="MIHLEQITDKPEMGVKATLDRPLDHLVACHRRIEERLEILERAA
                     VHLRDRRDEAMEAIENCFRFLDSNGMWHTADEEESIFPRLQGRLNETDAQFLRNLEAE
                     HREADELYGRLKELRAGLAAAVPGTLEGLIEDFGDVVRRLCEIYRRHIAAEDAHLIGI
                     ARAGLNESQLSEISREMKVRRGLNS"
     gene            10181..10723
                     /locus_tag="VLE22_04520"
     CDS             10181..10723
                     /locus_tag="VLE22_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSB13699.1"
                     /translation="MRLTAYTDYSLRVLIYLGLQNDRLATIADISRAYDISANHLMKI
                     IHNLGKLGYIQTIRGRGGGMRLAGKPGEINLGQLVRQVEPDFAIVECFDPNCRQDCVI
                     TPACRLQLVLDKAVQAFLDVLDRHTLADLLRNPTRLKELLQVDTTAIASRVEPGKQVR
                     RHRGTDSRRALEQPPSEARH"
BASE COUNT         2031 a         3257 c         3328 g         2140 t
ORIGIN      
        1 ctacggccag ttcctcgcag cggccgttat tttgctgctg gccggtgtga actacgtcgg
       61 cgtacgcttg agcggcggta tccaggtatc ggtcaccatc gttaaggtcg tcctgttggc
      121 cggcatcatc gttgtcggcc tctattgggg gcggggaaca atgggcaact acggaagctc
      181 cgcccctgcc atcggtggcg ttgcggggtt cttcgcggcc ctggtggcgg cactctgggc
      241 ctatgatggc tggaacaacc tgagtatggt cgcttccgag atcacgcggc ccaggcgcaa
      301 tcttcccatc gcgctgatcg cgggaacgat tgccgtgatg ggaatctacc ttctcgcgaa
      361 tctcgcctac ttttacgtgc tgcccgcggc ggcggtggct tcgagcgatc gcgtagcggc
      421 cgaaatgatg cgccggatcg ccggttccgg cggggctggc gcggtcagta tcgccgcaat
      481 ggtgtcaata ttcgccgcgc tgaacggctc aattctgtcc ggctcgcgcg tcccgtacgc
      541 catggcgcgg gacggcctat tcttctcgcc actcggacgg gtgcacccaa aacaccggac
      601 tcccggtacg tcaattttag tactcagcgt ctgggcggcc ctcctggtct tcagcggccg
      661 ttacgaggaa ttgttcacct atgtcatctt cgccagttgg attatgtacg gaatgacggc
      721 tgtctcggtg attgttttgc ggaaaaagag accgcattta tcgaggcctt accggaccat
      781 cggctatccc accgtaccag tactgtttgt gttgggtgcg gtctgcgtcg tcgtttcgac
      841 cctgctgaat tccccccggg aatcggtaat ggggctagta ctcatattgt tcggcctgcc
      901 cttttatttc cgttggaaac gacgcaaagt cgatgtagca taagggatgt attcggtgtc
      961 tgatgaaggg atttccgggg caaatttgcg tcctggaacc gttttgttct gaaatcggaa
     1021 caatgtcctt gaaatccctc gaaaaatgcg atacaaagct aggtgcgagt tggtaagcgc
     1081 actgcggaat tgttccattt tcggaaccag gtacgcggga cgaatgcgat gcatgggagc
     1141 aaatcgatgg acgtcgccaa aattctggcc gaattgcgcc aggaacggga acagattgaa
     1201 gaggccatcc tgagcctcga gcgattggca agcggacgcg gacggcggag agggagaccg
     1261 ccctcgtgga tggtagaggc gcgaaaacgg gcgcgccagg cgcccaaaaa ggctaagcct
     1321 gaaacaaatt caagaacagc cgccgcttga cagggcctaa attttcatcg ggtggggaaa
     1381 cggagcctgg aggaggttcc gtctcccacc tgggcagcct actggtacag gagatatttc
     1441 ttccgtagtt gaacaaattc ccgcagtttt tgttcctctt tccactggat ctctcgcggt
     1501 tcctctcccg tctccagtgc cgcgatggtc tcgacactgc cgatcaaccc gcggttggcc
     1561 tcgagcagga tctttcccgg gtagagtttt cccaacgccg cggcgatttc cagccccaac
     1621 cgttctccgc gaaaggcctc ccggtccgtg atgacgaacc gcacgccctc aatcagcgcg
     1681 ccttcgaagt tcgatgatgc cggacggaac cgtgtcggat aaaagcgcac gcctggaata
     1741 aactttgaat tcaactcggc cgccaactcg cgtccgttga tccagtccgc tcccacctgt
     1801 tcgaatggcg cgtccgtgcc acgtcctacc gagtagttgc gcgcatactc cagcagggct
     1861 acgcccggat agagcagcgc tgcgttgaag cttcgcatat tcggagaggg atcgacccag
     1921 acaagccccg tcgagtcgaa ccagtccccc cgttgccagc cctgcatcgc cacgacgtgg
     1981 agatccgcgc cgatgctctt ctcttcgttg aacaggcgcg ccagctcgcc ggccgtcatc
     2041 ccatgccgga gcgggagcgg gaaatagccg atgaacgact ccagttcctt gtcgagcacc
     2101 ggtccttcca cgtaaatgcc ggtaagggga ttcggccggt cgagcacata aaacgggagc
     2161 ttgcctcggg ccgcctcctc catcgcataa cccatcgtcg aaacgtaggt gtaaaagcga
     2221 acgccgatat cttggacgtc aaacaccaga acatccagat cccgcagcat ttcttcagtt
     2281 gggcgccggt tttgtcccga atacaggctc cagatcggga ggccggtggc cggatccgtt
     2341 gcgtggccga cgtccggtgt atcctcacgg cccgcgatcc cgtgctccgg cgaaaacagc
     2401 gctttcacgg caatgccggc ctccagcatg aggtcgacgt tacgccgtcc gttccggtcg
     2461 agtcccgtgt ggttggtgat caaaccgacg cgttttcccg ctagcggtgc aaagttctgg
     2521 gccgcgagga cgtccaaccc cgtaagcacc tgcccgtttc gggctacaac gcgacgcaga
     2581 ccggcctgtg cttcgttata cccggtgatg ctgatggccg ggaccttcat gtcgagcgac
     2641 gcggcaacca cggttgccac ccggcagcga agagaagtga tcggcggtct gagccgaggg
     2701 tggacgctat tcgcaagcag gatcacataa ctctgcgtcg ccggatcgat ccacagcgac
     2761 gttccagtga aacccgtgtg gccgtacgaa ccgatcggaa acaactcacc gcggttgccc
     2821 gagaacggcg aatcaatgtc ccaccccaga ccgcggagga tcggctggct gggcggagat
     2881 tgcggctcgg tgaacttcct caccactagc gggctgaaaa tgcgacggct ctccggctgc
     2941 cccatgccga gcatcatgcc ggcgaagcgc gagagatcgt cagctgtcgt aaagaggccg
     3001 gcgtgcccgg cgatgccgcc catgtagcgg ctcgtaggat cgtgaaccac gccgcgcagg
     3061 ggccccgcgt gcccgggaac cgtctccgtg ggcgcgatgc gccgccgcag ccgggcgggc
     3121 ggctggaaca tcgtgtcctt catccccaac ggccggaaaa cgtgttctcc ggcgtattca
     3181 ggcagcggat tcccgctgac ccgccggacg atctcgccca gcaggatgaa gttgatatcg
     3241 ctgtaaatga agcgcgtccc cgcgtcggcc acgggcgtgt ccgccagagc caacctgatc
     3301 cccgtctcgt accccttcca ttccggcttg aggtcgaggt ccggccgaag accggagaaa
     3361 tgggtcagca gatcgcggac ggtgatattg ctctttcctc cttggaactc gggcaggtac
     3421 acggtgacac ggtcattcaa tcgcagcttc ccgtcctcga acagcttcat gaccgcggag
     3481 gtcgtggcga caaccttggt cagggacgcg gcgtcgaaga tcgtgtccag cgtcatcttc
     3541 tcgcggcgag gcgtgagcga gcggaaccca tacgccttcc gatggacgat cttgccatgg
     3601 tgcgagacga gtagcacagc gcccgggatc tggtcttctt gaatcgctct ctcgatggct
     3661 gcatcgaggt cgcgagaacc tggaaacact ccccacgcgg gcgagagcgg agcgacaagc
     3721 actgccgccg ccaacagcaa gagtctggtt ttcattggag cttcttggcg atactctcca
     3781 gtttctccgc gagcgcgatg tccttcgagg taatccccgc ggcgtcgtgg gtcatcagat
     3841 cgaccgtcac gcggttccag acgttgaacc actcggggtg gtggcccatg gcctcgatgg
     3901 cgaccgccgc cgtggccatg aagccgaacg cgtgcacgaa gtcgggaaaa cggtactcgc
     3961 ggtgcagttt tccattgacg actttccatt cgggcagccc cttgagagcg gcctgaattt
     4021 ccgaatcgct gagcctggaa acgttggcca taatccccca tcttatgctg aaacggttcc
     4081 cacgcctgtg aaccgccagg tttgcatgca gctttcaggc cgcgacgaac ggcgcggaag
     4141 cccggccggc ctttttcttt atgatggtag aaggatgccg tcgcgacttc ctcgtctgag
     4201 aaccaacctt gacttcatgc cttcgccggt cgccgaccgc ccagggctcc tgatccggga
     4261 ttcgtatcag tactcggacg tcaccctgat cgtcccgccg attctcgtgc agtgccttcc
     4321 gttcttcgac ggtgaacaat ccgacctgga tctgcgccag gcgttggtgc agctcacggg
     4381 ggaaatccgc attggagacg ttcaggacca acttgtcgaa gcgctgagca aagcgggatt
     4441 tctcgaaaac gaagtgtatt ttcgcctgcg tgatgagaaa caccgtgagt tcgccgaagc
     4501 tggggtgcgc gaagcgaccc accagggctc agcctatccg gaagatcccg gcgagctgcg
     4561 ccaggtgctg ttgcggtatc tggaggacgg caagcctgcg cccgcgccgg gaactctcat
     4621 cggcatcgcg gcgccgcatg tcagccctga gggcggatgg ccgacgtatc aggctgccta
     4681 ccacacgttg cgggaagagt accgcgaccg taccttcgtg atcctcggaa cctcgcacta
     4741 cggcgccccg gatcgtttcg ggctgacgcg aaaaccgttc gttacacccc tcggccaggc
     4801 gcggaccgat acggtgctcg tcgacagaat cgcagctgaa gctccggagt cggttctgat
     4861 ggaggactac tgccacgcgg tcgaacattc gatcgaattc caggtcgtct tcctgcagta
     4921 cctcttcggt ccggatgttt gcattttgcc aatcctctgc ggatccttcg cgaagagcat
     4981 ctatgagggg ggacgccccg aggataccga agaagtgcgc cggttcctgg gcgttttggg
     5041 cgacatcgcg gcacgggaag gtgaccggct gttctgggtg ctgggaatcg acctcgccca
     5101 catgggacgc cgttacggcg accggttcga ggcgcaggcc ggacacggcg agatgctcac
     5161 ggtcgagcaa cgtgacaagg agaggatcga gcgggtcaag gaaggcgacg ccgccggctt
     5221 ctgggatcgg gtgcagcatc agcaggacga cctgaaatgg tgcggctcgt cgccgctata
     5281 cacgttgctc aaggccgttc ctcaggcgcg agcggatctg ctccgttacc agcagtggaa
     5341 tattgacgag caaagtgtgg tcagcttcgc cgcgttggcg tttcgccggt agccgagccc
     5401 ttcgaccgga ggccctccat cgagctctcc cggcgggtgc agaatggagg gattccaacc
     5461 cgatgaagaa acgcgacctc accatcgttt ggatcgcact gatcgggatt gtgttctggc
     5521 tcgtgacagt ccggccggcg ccgatccgca agccgctgcg cagggcgcag ttgtttatcg
     5581 agcggatcgt ccggggcaaa gagccgtccc aatccaaggc cggaaaggcc cgtgcgggag
     5641 caaagcggaa gacacccgaa aaatcttcgg ctcgcgaacc cgtcccgtaa gggccggcct
     5701 gaattcccgc ctctctttcc cggtaggccc caagcgtccc ggtggtgttg ccggaaactg
     5761 ttacaatttg attttccttg cccgctcgcg atgaggattt cccccaccat tgtgcttgcc
     5821 ctgctggcgg cgtgtctgtc cgccgatgcc gccggtccgc aagtttggaa tagcagcgag
     5881 ttcacctttt acgaaaccgg atggttccgc gcggacctcc tcggtgtggt gcggagccga
     5941 aatcatctga cggacgcgtt cgataaccgg ctgggcgcgc aggttgtctt tcccattcgt
     6001 ccacggctca gcatcggcgg cggctaccta aaacggtggg tcgatccgga tggccgcggc
     6061 gctcacctcg aggaccggat ctttcttgca ccgcggcttc tactggcgta ccgcccgatc
     6121 aagatcgagt cgttgacttc gtgggaacgc aatttctcgg ttccggggct gccggacttc
     6181 aaccgctacc gggagaccat cgacgtcgag aaagtgcggc atggcgcatc gccgttcttc
     6241 ttcgaacaag tgtcgtttcg agattacgga cttgcctgga cccgtaccgc ggtcggcctg
     6301 cgatggcggc ttcattccgg gttccgcttc gaggccgggt atcagttcga aagccagaag
     6361 attgccggcg cctgggcgcc gcggcacgct atccggacag tgatcggatt ccgtaagccg
     6421 aggccgaact aaggacgcca cgactgggtc cggcggatca ccagatgtac tcctttgccg
     6481 tgtcccggcg ggcgtgactg tcgagcacgt gtaccacccg gatcttcttc agcttggtcg
     6541 gcgaaagctg cccgatcggg atgtagagga tcgatcgatt catgcgtgag gcgatggagc
     6601 gaaagatcga gcgcggcggc ttcgcggcga cgtagacgac gtggcggtgc accgagtaat
     6661 cgaggcccgc cagcagcagc cgttcgggtt tgttccgcgc aaggtcgtag tcgggatcgc
     6721 cccacacgtc gaacatccgc aacggcggca aactcatcag aaagccacca tactcggcgc
     6781 gcccgacgcc gggaccgacc agattgccga acgggtccgt cgagtagaag gccatatccg
     6841 actcgttctg gtgctcgccg agccaggtcg tcaggtatgg gtagcgatcg tcgcggtcct
     6901 cgtcgaaaat gacgaccacc gagcccactt cgccggcgat cttgtcaaac tgccgcacgt
     6961 agatcctgct ctcgtgccag ttgcggattg tctcccgtag gtcgataccg tcgaggatgg
     7021 acgtggtgaa aggctcagtg cggacgcgct cctccgagag aatactcttc gctttcttct
     7081 ggagaaagcg gccgtagttc tccaccacga gatcctcggg cggataggag cagatggcgg
     7141 agccgtcgat ctggcgcgcc cactcgccgg gaaacttctc ctttttgcgc ggcttcaggc
     7201 ccaccgggcg gagtcgctgc ttggggcggg gaagacggcg ccgcaggcgg atctttttcg
     7261 tgtcgagcca gacctcttca ccggaaagat tcaccgtgac gacatcgctg gcggttttct
     7321 gtggtggata cttaccggct gtttcccaga cgtcccaggc gtagttgtcg tcgacgacgc
     7381 agcgcgcggc gaccgtgata tcgaacagcc ccgcggtcag gtcgcccgcg gaaagcgcca
     7441 ggttgcgggt gtagcgcgcc atcaggcgcc gctgccagtg ggccaccctt tcgcctgtgg
     7501 cggtctcata agacttctcg gcgtcgcgca ggacggcgaa ctgagtgcgg cggcggtcgg
     7561 cgagtgccac actcgtcatt tcaacgcgga actgctcata gcggtactgc agatacgggt
     7621 attcgacggt gatctccgcg aggcaatcgg gatgcgggtt cagtacttgt acgccggaac
     7681 gccgctggcg ggacatcggc tgcgcctggg gctcctccat ggcatcgagg accgggtcga
     7741 gactgttaag cgagagaacg acgaatgcgg ccgcctctga attgaggccc tgaagcttcc
     7801 aggcgatgcc tcggccctgc gcggtcagct tctccgatcg cggctgagga aacagccggt
     7861 aggcctcgat gtacttctcc aggcccgtcg agtaaaggga gtagggatcc gggtaacggt
     7921 ccgggacatg aggcttctca ccgaaatcgg gttcaatgaa gacaatctcg gcgccgatct
     7981 cgagggcggt gcggaccgct tcggtgaacg ggtcggcggg ctccaccggg acgtaaatcg
     8041 cgcggtcgtc ctcgccgtct tcttccccgt agactacgac agatatttcg ggcaggcggc
     8101 cgactgcgct caagtaggcc tgttcaagag tgacgggcag ctcgacagcg acgatccccg
     8161 gcctctcgcg cagaagcgcg cgccgcagct caaccgcgaa ctcaaggcgt ccgggagcca
     8221 cgggaacata cgtgaggtgg ccacggcgcc ggctgccggc ttcgaacgga aacttgtcgc
     8281 cggacggctc agattcgctc gacataccgc atcgcctctt cgccgagcgt ctggaggatg
     8341 gcggtttcga tcgcctggcg ctcgtggaag ccgttcgcag aaagcccggt gagcttcatc
     8401 gcgaagcgcg cgatgttgat gccgtcccgg acggtatagc gttcgtcggc ggagtgcgcc
     8461 tgctgaagga agtcggtcac gtactggaga attcgttcct cgccgaaggg caggttctcg
     8521 cgcaggattt ggtactcttc gtcgcgctcg gggaagtcga tcaggatctg cggctggagg
     8581 cgtgagtgga tgtattccgg caggtcgaac gtgctggcgt cgtcattgag cgtggcgaca
     8641 aagcgaaaat tcgggttcgc tttgatcttt attccggcca cgatggattc gacatatcgt
     8701 cgcgtgtcga gcagcggcgc gaggctggcc cagctcttct cgctcatccg gttgccttcg
     8761 tcaaggatta cgacgccgcc cctcagcatg gcggtaacga gaggcgaggc gacgtaacga
     8821 agtttgcctt cgccttcgat caccggcgtg accagcagat cctccgggcg cgtgtccacc
     8881 gtagcttgca tgatgtagac gtcgcgtccg aggcgccggc cggcggcata agcgagcgtt
     8941 gtcttgccca cgccgggctt gccgaccaga cgcggattca tgggcacgtc ctgcgggtcc
     9001 acgaccagcc acgccgcgag cagttgccgc atgacttcgt cctgtccgac ccagcggccg
     9061 ggcaactcgt cgggatgagc caaatgcagc gcaacgccct cgatctcgac caccatgacg
     9121 actattgtcc taaaatcgga ggcgttcggg tgtgtgacgc tgctgtctct acggctcggc
     9181 tactttgcca ggtactccag taaggcgacg gcgttgttgt gcgcagtgtc cttcgccgca
     9241 aagaggagcg tgatcgcacc tttccggccg cgtgatcgga tctcattcag cgtatcggca
     9301 tgtttgctca gctcgatctg gtagcggcgt ttgaactccg accagcgctc tggctcgtgt
     9361 ccgaaccact ttcgtaactc ggtgctcggc gccacgtgct tcagccacag atccacagcc
     9421 gccttctctt tggacatacc cctcggccag acgcgatcga cgagaatcct cagcccatct
     9481 cccttagccg gcggatcgta gatacgcttt acgcggatca tctttcgtcg ctgattccag
     9541 ttgattcgaa cagctaaaac aagtatacta catacatcta tagtctccaa gattgaaaag
     9601 ttggaaccgc ataatgattc acctcgaaca gatcactgac aaaccagaga tgggggtcaa
     9661 ggccaccctc gatcggccgc tcgaccactt ggttgcctgt catcgacgca ttgaagagcg
     9721 tttggagatc ctggagcggg cggcggtcca tctccgcgac cggcgcgatg aggccatgga
     9781 ggcaatcgaa aattgcttcc gatttcttga ctccaacgga atgtggcata ccgccgatga
     9841 ggaggaaagt atcttcccga ggttgcaggg cagattgaac gaaaccgacg cgcagtttct
     9901 tcgaaacctc gaggcggagc atcgggaggc ggacgagctg tacgggcggc tcaaggaact
     9961 tcgagccggc cttgcagccg ctgttcccgg gaccttggaa ggcctgattg aagacttcgg
    10021 ggatgtcgtc cgccgcctgt gcgagatcta ccggaggcat atcgccgcgg aagacgcgca
    10081 tttgatcggc atcgcccgcg ccgggctaaa cgaaagtcaa ctctccgaga tctctcgaga
    10141 gatgaaggtc cggcgcgggc tgaactcgta agagcttccc atgcggctca ccgcttatac
    10201 cgactacagc ctccgagtgt tgatttacct gggtcttcag aatgaccggt tggctactat
    10261 cgctgacatc tcgagagcgt atgacatctc cgccaaccac ttgatgaaga tcatccacaa
    10321 tctgggcaaa ctgggctata tccagaccat tcgcggccgc ggcggcggga tgaggctagc
    10381 cggaaaaccc ggagagatca acctcggaca actcgtgagg caagttgagc ccgactttgc
    10441 catcgtggag tgttttgatc ccaactgccg tcaagactgc gtgatcacgc ccgcgtgcag
    10501 attgcagttg gtccttgaca aggcggtgca ggcctttctg gatgtcctgg atcgccacac
    10561 cctggccgac cttcttcgga atccaaccag gctcaaggag ctgctgcagg tcgacacgac
    10621 ggccatcgca tcacgggtcg aaccgggaaa gcaggtgcgg cggcaccgcg ggacagacag
    10681 ccgccgggcg cttgaacagc ctccctcgga ggcgcgccac taaatcgtag gccgcatcgc
    10741 gcagcggggc ggggac
//
LOCUS       DATIFQ010000103         2656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2139808, whole genome shotgun sequence.
ACCESSION   DATIFQ010000103 DATIFQ010000000
VERSION     DATIFQ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139808"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VLE22_04525"
     CDS             complement(<1..223)
                     /locus_tag="VLE22_04525"
                     /inference="COORDINATES: protein motif:HMM:NF020859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HSB13700.1"
                     /translation="MLLIQVAKARQVRVDGIHFQSLRYISTTLAAYVGETVTLRFDPR
                     DMAEIRVFHGDRFLCQAVCPELAGATVPLR"
     gene            complement(247..1716)
                     /locus_tag="VLE22_04530"
     CDS             complement(247..1716)
                     /locus_tag="VLE22_04530"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HSB13701.1"
                     /translation="MPKQRWVELFQEALRPGTPATSGAAALDRELASCRFCDPRLERR
                     FRRLVGQLATRLGQTIPLACQDWTNTKAAYRFLSNRRVDEMAILGGHFQATRERFAAA
                     GGPILVLHDTTEFSYTRESKEAIGILYKSCGRKDKEGRSRLHTLCGILMHASLAVTAE
                     GLPLGLAAIKFWTRSKFKGTNALKRKVNPTRVPVEAKESVRWLENLRQSTKLLDEPSR
                     CIHIGDRESDIYELFCAAQESGTKFLVRTCVDRLAGDGRHTIAALMRRIKVKAIHQVE
                     VRDAKGTVSQATVKVKYHRLRVYPPIAKQKQYPPLNLTVIYAQESSTPRNRDRIDWKL
                     ITNLPVRSRKEAVEKLAWYAMRWRIETFHKILKSGCRAEASKLRTAERIVNLIAMFCV
                     LSWRIFWMTMMNRVAPVASPLVALTRVETQLLDRLLPEASKRRKATLSTYLIKIAKLG
                     GYLARTKDSPPGNMVMWRGLSRLTDIELGFLLGVRVVGN"
     gene            1834..2163
                     /locus_tag="VLE22_04535"
     CDS             1834..2163
                     /locus_tag="VLE22_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB13702.1"
                     /translation="MAEIIGNADARKADEWRERLAEQDRSGLTVQQFCKERGVSTWSF
                     YTWRKRLREADSGAVRFALIDRAAEPIELRNTADLEVVFATGDRLLIRSGVDAAILRT
                     VLRALRG"
     gene            2160..2354
                     /gene="tnpB"
                     /locus_tag="VLE22_04540"
     CDS             2160..2354
                     /gene="tnpB"
                     /locus_tag="VLE22_04540"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HSB13703.1"
                     /translation="MIHLPASVRVYLCLTACDMRKSFDGLHALVRDHLELDPFGGHLF
                     VFASRRRDRLKILYWGVSRM"
BASE COUNT          555 a          764 c          778 g          559 t
ORIGIN      
        1 ctcgaagagg aaccgtagcc ccagccagtt cagggcagac cgcctggcat agaaatcgat
       61 ccccgtggaa aacgcggatt tcggccatgt cgcgcggatc aaagcgcagc gttaccgttt
      121 ccccgacgta cgccgctaac gttgtcgaga tgtaacggag gctttggaag tggatgccat
      181 cgacgcgcac ctgccgggct ttggccacct ggatcagcag caaatcgtaa gcgtccggtg
      241 aaccttttaa ttacccacaa ctcgcacccc caagaggaat cccaactcga tatcagtgag
      301 ccgagacaga ccgcgccaca tgaccatgtt tcccggtggg gagtctttcg tccgggcgag
      361 ataaccgcct aatttggcga tcttgatgag ataggtggat agtgtcgctt ttcgacgctt
      421 ggatgcttca gggagcagtc tgtccaacaa ctgagtttcc acacgggtca gcgcaaccaa
      481 gggcgatgct acgggcgcta cgcggttcat catcgtcatc cagaagatcc gccaactgag
      541 aacacagaac atcgcaataa ggttgacgat cctttccgca gtccgcaact tcgatgcttc
      601 tgcccggcag ccggatttca ggatcttatg aaaggtctcg atgcgccacc gcatcgcgta
      661 ccaggccaac ttctccaccg cctccttacg gctgcggacc ggcaggttgg tgatcagttt
      721 ccaatcgatc ctgtcccggt tccgtggcgt tgacgattcc tgagcgtaaa tcacggttag
      781 attcagcggc gggtattgct tctgtttggc gatcggcgga tatacccgca accggtgata
      841 cttcaccttt acggtcgctt gcgaaaccgt ccccttggcg tcgcgaacct cgacctggtg
      901 gatcgccttc accttgatcc gccgcatcag tgcggcaatc gtgtggcggc catcaccggc
      961 caggcggtcc acacaggtgc gtaccaggaa tttcgtgccg ctttcctggg ccgcgcaaaa
     1021 cagttcgtaa atgtcgctct cgcgatcccc gatgtgaatg caacgggatg gttcgtccag
     1081 caacttggtg gactgcctca aattctccaa ccagcgcaca ctctccttgg cctcaacggg
     1141 tacgcgagtt ggattcactt ttcgcttcag tgcattggtc cccttgaatt tactccgagt
     1201 ccagaacttg attgccgcca gtccgagtgg cagcccctcg gcagtaacgg cgaggctggc
     1261 gtgcatcaag atgccgcaga gcgtgtgaag ccgggaacgt ccctctttgt ccttcctgcc
     1321 acacgatttg tacaggatgc cgatcgcttc cttggactcc cgcgtataac tgaattccgt
     1381 cgtgtcatgc agcacgagaa tagggccacc cgccgcggcg aatcgttcac gggtggcctg
     1441 aaaatgtccg cccagaatcg ccatctcatc aacccgtcga ttcgaaagga accgatatgc
     1501 tgccttcgta ttcgtccaat cttggcaagc caacggaatg gtctggccga gtctggtggc
     1561 cagttgtcca accaacctcc ggaatcgccg ttcgagtctc gggtcacaga accggcaact
     1621 cgccagttcc ctgtccagag ccgccgcccc gctcgtggca ggagtgccgg ggcgcagagc
     1681 ctcttgaaac aattcaaccc atctctgttt tggcaccttg gcgcttcatt ctacacctca
     1741 aggcaacggc aagtcgcggt caaaagttgt gggtaattaa aagccggtga acacctctat
     1801 ccgactggcc cgagcgcact gcatagtatg gcaatggcgg aaatcatcgg gaacgcggat
     1861 gcgcggaaag cggacgaatg gcgcgaacgg ctggctgagc aggaccgcag cgggttaacc
     1921 gttcaacagt tctgtaagga acgtggagtg agcacgtggt cgttctacac gtggcgcaag
     1981 cggctacgcg aagccgactc cggcgctgtt cgcttcgccc tgatagaccg cgcagccgag
     2041 cctatcgaac tcaggaatac ggccgacctg gaagtcgtat tcgcgaccgg agatcgactg
     2101 ctgattcggt ccggggtgga cgctgcgata ttgcgaactg tactcagggc gttgcgggga
     2161 tgattcacct gccggccagc gtgcgcgttt acctgtgtct gacggcctgc gatatgcgca
     2221 agagctttga tgggctgcat gcgctggtgc gcgaccacct cgagttggat ccgttcggcg
     2281 gacacttgtt tgtgttcgcc agccggcgac gtgatcgctt gaagatcctg tactggggtg
     2341 tgtcacgaat gtgagtgaaa acttgcagag ctgtaccgaa gatgggagac ggcctcccgg
     2401 agtcggcttt caggagttgg cttcaaacca cccggagctg ctgcgttcaa aaggcgtggt
     2461 ggtgaacgtc cgaggaaaag gcgtccgaag agggcgtcag gtgagcgttg agaagatgag
     2521 cgaaagcgaa ccgtccgatg acgcatcgtc aaagattcaa ggaactgtcg gaactggagc
     2581 cgcccagtag ctccaggatg agccgggaag gagcctgttt actggtccgg cggcagtcgg
     2641 tgtataggcg gcatga
//
LOCUS       DATIFQ010000104         2386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_417560, whole genome shotgun sequence.
ACCESSION   DATIFQ010000104 DATIFQ010000000
VERSION     DATIFQ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417560"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VLE22_04545"
     CDS             complement(<1..708)
                     /locus_tag="VLE22_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13704.1"
                     /translation="MRFALIFALALAAPLAGQRITVPLDGQWNIDESVSLNETPASFR
                     HTVPVPGMANLAAPPFPDVDLFDSREIINNRVRKGVLPESAIVTNAGISRQKRNYFWY
                     EKRFRVPARKEVAILKINKAQFGTAVWLNGRKSGEHLSCFTAGYFNVTDAIDWNGENR
                     LVVRVGAHPGVLPEGIPAGTDFEKLKWTPGIYDSVSLLLGDNPIIETVQVAPRIKSSE
                     IIVETKLRNYGAARAAEL"
     gene            818..1561
                     /locus_tag="VLE22_04550"
     CDS             818..1561
                     /locus_tag="VLE22_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB13705.1"
                     /translation="MDTGNDIAIRAVDLAKHYRSGETDLIVFSGLGFEVWRGERLAIV
                     GESGAGKSTLLHLLGGLDRPSNGTIHFGAKEITALPDTELADFRNREIGFVWQIHYLL
                     PEFTALENVMMPLLIRGASHEEAARPSLARLEEVGLTARASHRAGELSGGEQQRVALA
                     RALVGNPACLFADEPTGNLDSRTGEMVFGLLGELCRSRRLTTVIVTHNFSFARRCDRV
                     LKMEGGALGPAAEASLKEPSRQEDGKNYV"
     gene            1554..>2386
                     /locus_tag="VLE22_04555"
     CDS             1554..>2386
                     /locus_tag="VLE22_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HSB13706.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGSPYIETEHLLLGLLREDKAL
                     ANRFLRSHAAIESIRKQIEAHTTVREKVPTSVDLPLSHECKRVLAYAAEEAERLNHKH
                     IGTEHLLLGLLREEKSFAAEILHERGLRLSQVREEIARSTSEKMSSSRPKESSLLSEF
                     SRDLTQSAMDGVLDPLIGRDHEVERVVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQR
                     IAEGDVPSFLSDKRILALDLSLIVAGTKYRGQFEERLKTIMKELMENQNAIIFIDELH
                     TL"
BASE COUNT          451 a          694 c          827 g          414 t
ORIGIN      
        1 cagttcggcg gcccgtgcgg cgccgtaatt tcttagcttc gtctcgacga tgatctcgga
       61 tgacttgatc cggggagcca cctggacggt ctcaatgatc gggttgtccc cgaggagcag
      121 cgagacgctg tcgtagatgc cgggagtcca cttcagcttc tcgaagtcgg tcccggcggg
      181 gattccttct gggagcactc cgggatgggc gccaacgcga accacgagcc ggttctcgcc
      241 gttccagtcg atcgcgtccg tcacgttgaa gtagccggcc gtaaagcagc tcaggtgttc
      301 accggacttg cggccgttca gccacaccgc cgtgccgaac tgggccttgt tgatcttgag
      361 gatggcgact tccttccggg cgggaacccg gaagcgcttc tcgtaccaga agtagttcct
      421 cttctgacgc gagatgccgg cgttggtgac aatcgccgac tccggcagca cgcccttccg
      481 gacgcggttg ttgatgatct cgcggctgtc gaacaggtcg acgtccggga atggaggagc
      541 ggcaaggttg gccattcccg gaacggggac ggtatggcgg aacgaggcgg gcgtctcgtt
      601 gagagagacg ctctcgtcga tattccattg gccgtcgagg ggcacggtaa tccgctggcc
      661 ggcgagcggg gccgcgagag cgagggcaaa aattagagca aaacgcacga tgaaacctcc
      721 agtgttatcc ggaccgttgt accgcacaaa acgcctcaaa agccacagcc gaggctcgct
      781 ttcgtgttag cgtagaggaa agggggttgg gcggcgattg gataccggca acgacatcgc
      841 tatacgggcc gtcgatctcg ccaaacacta ccgctcgggc gagacggacc tgatcgtgtt
      901 cagcggtctc gggttcgaag tgtggcgggg cgaacgtctg gcgattgtgg gtgaatccgg
      961 ggccggaaaa tccaccctgc tgcatcttct cggcggtctc gacaggccat cgaacggaac
     1021 gatccacttc ggcgcgaagg agatcacggc gctgccggac acggaactgg cggacttccg
     1081 gaaccgcgag atcggttttg tctggcagat ccattatctg ttgcctgagt tcacggcgct
     1141 cgagaacgtg atgatgccgc tgctgatcag gggcgcctcc cacgaggagg ccgcccggcc
     1201 gtcgcttgcg cggctggagg aagtgggcct cacggcgcgc gcctcgcacc gcgcgggcga
     1261 gctttccgga ggcgagcaac agcgggtcgc gctggcgcgg gcgctggtcg ggaatccggc
     1321 ctgtctcttc gcggacgaac cgaccggaaa cctggactcg cgaaccggcg agatggtctt
     1381 cgggctgctc ggggagctgt gccggtcccg gcggctgact acggtgatcg tcacccacaa
     1441 tttttcgttc gcccggcggt gcgaccgcgt cctgaaaatg gagggcggcg cgctcggccc
     1501 cgcggcagaa gcatccctga aagagccgtc aaggcaggag gacggaaaaa actatgtttg
     1561 agcgatacac ggaaaaggcc cgcagggtga tctttttcgc gcgctacgag gcgagccagt
     1621 tcggcagccc gtacatcgag accgaacatc tgctgctcgg gctgctgcgc gaggacaagg
     1681 ctctggcgaa ccgctttctg cggtcgcacg cggccatcga atcgattcgg aaacagatcg
     1741 aggcgcacac caccgtccgt gagaaggtgc cgacctcggt ggatctgccg ctgagccacg
     1801 agtgcaagcg ggtgctggcg tacgcggcgg aggaggccga gcggctgaac cacaagcaca
     1861 tcggcacgga gcacctgttg ctcgggctgc tgcgcgagga gaagtcgttc gcggccgaaa
     1921 ttctgcatga gcgcgggctg cggctgtcgc aggtgcgcga ggagattgct cgatcgacgt
     1981 cggagaagat gtcgtcgagc cggccgaagg agagctcgct gctctcggag ttcagccggg
     2041 acctgacgca gtccgcgatg gacggggtgc tggacccgct gatcggacgg gatcacgagg
     2101 tggagcgggt ggtacagatc ctgtgccgcc gcacgaagaa caacccggtg ctgatcggcg
     2161 agccgggcgt gggaaagacg gccatcgtcg aaggcctggc acaacggatc gccgaagggg
     2221 atgtgccgag cttcctctcg gacaagcgaa ttctggcgct ggacctgtcg ttgatcgtcg
     2281 ccggcacgaa gtaccgcggg cagttcgaag agcggctcaa gacgatcatg aaggagctga
     2341 tggagaacca gaacgccatc atcttcatcg acgagctgca cacgct
//
LOCUS       DATIFQ010000105         4426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2332766, whole genome shotgun sequence.
ACCESSION   DATIFQ010000105 DATIFQ010000000
VERSION     DATIFQ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332766"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VLE22_04560"
     CDS             <1..1157
                     /locus_tag="VLE22_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13707.1"
                     /translation="LFHASADGASADTYLPWEGGPAYYRKWSNGVSADPGYFPISVWL
                     QDPKYAQEYRAIGINLYVGLWRGPNDEQLAALAAAGMPVLAGQRKPAMASLNSRMIRG
                     WTIGDEPDNAQSKPGGGYGPCIFPPAIVDQYRGIQSADATRPVYLNLGQGVANDAYVG
                     RGVCRGHSEHYAEYIRGADIVSYDVYPVNSKYPLWWVGEGVERLRRWGEHKKPVWNWI
                     EASAIQGERKPSPAQIRSEVWMSIIHGSMGIGYFCHQFKPVQDEAAPLHDPETRQALA
                     DINGQIASLARVLNTPPVGNGVTVASSNPDSPIDVMLKRQGGKTYLFAMGARPGGETT
                     ARFKLRDCGDVRATVLGESRTIPVTGGIFEDRFADYQVHLYELPFDPGAR"
     gene            1306..2241
                     /gene="argF"
                     /locus_tag="VLE22_04565"
     CDS             1306..2241
                     /gene="argF"
                     /locus_tag="VLE22_04565"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSB13708.1"
                     /translation="MASANAIWQIQARRLTFDLDLKREELAYLLDLTEQVKRKPGRYA
                     QSLAGHYLSLLFEKPSLRTRLTFELAIKQLGGDSVLSEGPIGSREPLKDVARNLDRWT
                     NAIVARTFSQKTVDDLAKWSSVPVINALSDVYHPCQALADVFTLKEQYGDLRGLKVAF
                     AGDGNNVAHSLMLTAARLGASFTIACPAGYEPKAEVVAEAQELAAETGAAVTVTSDPE
                     EAVRDADAVYTDVWASMGQEGQLDQRRRDFQPYQVNETLFAIAKPSAVFMHCLPAKHG
                     EEVTDGVIESPQSVVFDQAENRLHTQKALLLMLLG"
     gene            2353..3564
                     /locus_tag="VLE22_04570"
     CDS             2353..3564
                     /locus_tag="VLE22_04570"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSB13709.1"
                     /translation="MSKPKKVALAYSGGLDTSIIIPWLKENYQCEVVAVCGDIGQGGD
                     ELTGLREKALKTGASEVHVEDMREEFVSEYLWRLVRAGAVYEHKYLLGTSVARPLLAK
                     RQVEVALRTGCDALSHGCTGKGNDQVRFELTYKALAPHLAVIAPWREWDINSREDAIE
                     YAAKHNVPISATTTKIYSRDRNIWHISHEGGALEDPKNAAPEDVWLLTKSPREAPNTP
                     AEVTIGFEQGRPVSVNGQHYVSAVALLETLNEIGAEHGIGRIDLVENRFVGMKSRGCY
                     ETPGGTLIMAAHRELEALTLDRSTLHYKQKLSVDYAELVYNGLWFTPLREALDNFFEA
                     TARTTTGEVTLRLYKGTIEPVCRKSPYSLYSLDIASFTMGASYDQKDALGFINLIGLP
                     IRIRAAVTKKS"
     gene            3561..>4426
                     /gene="argH"
                     /locus_tag="VLE22_04575"
     CDS             3561..>4426
                     /gene="argH"
                     /locus_tag="VLE22_04575"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSB13710.1"
                     /translation="MKLWGGRFEQGPSEIFERFSGSLHFDRRLIGADIRGSQAFARAL
                     ERVGILKAEERERIVEAFDAILAESERPGFFDGAADEDVHTLVIRKLKERTGELAEKI
                     HTGRSRNEQVSLDTRLWLREEIGRIEELLSGFLAALLGLAKRYPDAVLPGFTHMRRAQ
                     AVLWPHYVLAYFEMFARDWERLEQARRSANLLPLGSGALAGSGFPFDREAMARDLGFD
                     GVTANSMDVSSDRDFLLDFLYAASTAMLHLSRLAEDWILYSSEEFGWLELGDEVTSGS
                     SLMPQKKNPDSL"
BASE COUNT          859 a         1442 c         1399 g          726 t
ORIGIN      
        1 cacttttcca cgcgagcgcc gatggagcct cagccgacac ctacctgcca tgggaaggcg
       61 gcccagccta ctaccggaag tggtcgaatg gcgtctccgc ggatcccggc tatttcccga
      121 tcagcgtgtg gctgcaggat cccaagtatg cgcaggaata cagagccatc gggatcaacc
      181 tttatgtcgg gctctggcgg ggacctaacg acgagcaact cgcggccctg gcggcagcgg
      241 ggatgccggt gctggctggc cagcggaagc cggccatggc gagtctgaac agccggatga
      301 tccggggttg gaccattggc gacgaacccg acaacgccca gtcgaaaccg ggcggcggct
      361 acgggccctg cattttcccg cccgcgattg tcgatcagta tcgcggcatc cagtcggcgg
      421 atgccacccg gcccgtgtac ctcaatttag gccagggcgt cgccaacgac gcttacgtgg
      481 ggcgcggagt ctgccgcggg cacagcgaac attatgccga atacatccgc ggcgctgata
      541 tcgtttccta cgacgtgtac cccgtcaaca gcaagtatcc tctgtggtgg gtgggagagg
      601 gtgtcgagcg tctgagaagg tggggagagc acaagaaacc ggtgtggaac tggattgagg
      661 ccagcgcgat ccagggcgaa cgcaaaccga gtcccgcaca aatcagatcc gaagtgtgga
      721 tgtcgattat ccacggctcc atgggtatcg gctatttctg ccaccagttc aagcccgtgc
      781 aggatgaagc ggcgccgctt catgatcctg agacgaggca ggcgctggcg gacatcaatg
      841 gccagatcgc ctccctggcc cgcgtgctga acacgccgcc ggtgggaaac ggcgtgaccg
      901 tggcctcctc caatccggat tcgccgatcg acgtgatgct gaagcggcaa ggagggaaaa
      961 cctacctgtt cgcgatgggc gcgcgccccg gcggggaaac cacggcgaga ttcaagcttc
     1021 gcgactgcgg cgacgtacgc gccaccgtgc tcggcgaatc caggacgata ccggtgaccg
     1081 gcgggatttt cgaagaccgc ttcgccgact accaggtaca cctttacgag ctgccattcg
     1141 accccggcgc ccggtgaagc ttgtcagctc aatctgagcc gcgactgaaa ggagcggtca
     1201 aaatggttcc cccaccttga cgtccgcgct tccggtgtgt aattattcag tagggtgaat
     1261 aaaaatacag cgccggccag gagcaagacc gcccgtgtct cgtccatggc gtcggccaat
     1321 gccatctggc agatccaggc gcgcaggctg accttcgacc tcgacctcaa gcgggaggaa
     1381 cttgcctacc tgctcgacct caccgagcag gtcaagcgca aacccggccg ctatgcgcag
     1441 tcgctcgcgg gccactacct cagcctcctg ttcgagaagc cctccctgcg cacccggctg
     1501 acgttcgagc tggccatcaa gcagcttggc ggcgactctg tgctgagcga aggccccatc
     1561 ggaagccgcg agccgctcaa ggacgtcgcc cgcaacctcg accgctggac gaacgccatc
     1621 gtcgcccgca cattctcgca gaaaacggtc gacgacctcg ccaagtggtc gagcgtcccg
     1681 gtaatcaacg cgctgagcga cgtctaccac ccctgccagg cactcgccga cgtcttcacg
     1741 ctgaaggaac agtacggcga cctgcgcggc ctcaaggtcg ctttcgccgg cgacggcaac
     1801 aacgtcgccc actcactgat gctgaccgcc gcccgcctcg gcgcgagctt caccatcgcc
     1861 tgcccggcag gctatgagcc gaaagcggag gtcgtcgccg aagcccagga actcgcggcc
     1921 gagaccggcg cggccgtcac cgtcacaagc gatccggaag aagcggtccg cgacgccgac
     1981 gccgtttaca ccgacgtctg ggccagcatg ggccaggaag gacaactcga tcagcgccgc
     2041 cgtgacttcc agccctacca ggtcaacgaa acgctgtttg caatagccaa gccgtcggcc
     2101 gtgttcatgc actgccttcc ggccaagcat ggagaagagg tgaccgacgg cgtgatcgaa
     2161 tccccgcagt cggtggtgtt tgatcaggcg gaaaaccgcc tgcacaccca gaaagccctg
     2221 ctgctgatgc tgctgggcta ggctggttgt tttgtggcta gcagggcgct aaaaaacccg
     2281 cgccacaggc tgaaggctgt gtcgggcgca aacaccggcg ggtttttcag caccctgtta
     2341 gaatcccaag ttatgagtaa accgaagaaa gttgcactcg cctactcggg cggtctggac
     2401 acgtccatca tcattccgtg gctgaaggag aactaccagt gcgaagtcgt cgccgtgtgc
     2461 ggcgatatcg gccagggcgg cgacgagctg accggcctgc gcgagaaggc gctcaagacc
     2521 ggcgcgtccg aggttcatgt cgaggatatg cgcgaagagt tcgtcagcga gtacctgtgg
     2581 cgtctcgtcc gcgccggcgc ggtgtacgag cacaagtatc tgctgggcac atcggtggcg
     2641 cggccgctgc ttgccaagcg gcaggtggaa gtggcgctgc gcaccgggtg cgacgcgctg
     2701 tcgcacggct gcaccggcaa ggggaatgac caggtgcgtt tcgagctgac ctacaaggcc
     2761 ctggctccgc accttgccgt catcgctccg tggcgcgagt gggacatcaa ctcgcgcgaa
     2821 gacgcgatcg agtacgccgc gaagcacaac gtccccatct ccgccacaac cacaaagatc
     2881 tacagccgcg accggaacat ctggcacatc tcccacgaag gcggcgccct cgaagatccg
     2941 aagaacgccg cgcccgagga cgtctggctg ctgacaaaga gcccgaggga ggccccgaac
     3001 acgcccgccg aggtgacgat cggattcgag cagggccgtc cggtctccgt gaacggccag
     3061 cactacgtct ccgccgtcgc gctgctcgaa acgctgaacg agatcggcgc cgagcacggc
     3121 atcgggcgta tcgacctcgt cgagaaccgc ttcgtcggca tgaagtcgcg cggctgctac
     3181 gagacgcctg gcggcacgct gattatggcg gcccaccgcg agctcgaagc gctcacgctc
     3241 gaccggagca cgctgcacta caagcagaag ctctcggtcg attacgccga actggtctac
     3301 aacggcctct ggttcactcc gctccgcgag gcgctcgaca acttcttcga agcgacggcc
     3361 cgcaccacga ccggcgaagt gactctgcgg ctctacaagg ggaccatcga gccggtgtgc
     3421 cggaagtcgc cgtactcgct ctactcgctc gacatcgcca gcttcacgat gggcgccagc
     3481 tacgaccaga aggacgcgct cggattcatc aacctgatcg ggttgccgat ccgtatccgc
     3541 gccgccgtca cgaagaaatc atgaagctct ggggcggaag gttcgagcag ggtccgtcgg
     3601 agatcttcga gcgcttctcc ggctcgctcc acttcgaccg gaggctgatc ggcgcggaca
     3661 tccgcggctc gcaggcattc gcgcgggcgc tggagcgcgt cggtatcctg aaggccgagg
     3721 agcgggagcg catcgtcgag gccttcgacg cgatcctcgc ggaatcggaa cgccccggct
     3781 tcttcgacgg agccgccgac gaagacgtcc acacgctcgt catacgcaag ctgaaggagc
     3841 ggacgggcga gctggccgaa aagatccaca ccggccgcag ccgcaacgag caggtgtcgc
     3901 tcgacacccg gctatggctg cgggaggaaa tcggccgcat cgaggaactg ctctccggat
     3961 ttctcgccgc gttgctaggc ctcgcgaagc gctatccgga tgcggtgctt cccggcttca
     4021 cgcatatgcg gcgcgcgcag gcagtgctct ggccgcatta cgtgctcgcc tactttgaaa
     4081 tgttcgcgcg cgactgggag cggctggaac aggcgcggcg gagtgcgaac ctgctgccgc
     4141 ttggctccgg agcgctcgcc ggcagtggct tcccattcga ccgcgaggcg atggcgcgcg
     4201 accttggctt cgacggcgtc accgccaaca gcatggacgt ctcctcggat cgggattttc
     4261 tgctcgactt cctttatgcc gcctcgaccg cgatgctcca cctgagccgc ttggccgagg
     4321 actggatcct gtactccagc gaagaattcg gctggctgga gcttggcgac gaggttacca
     4381 gcgggtcgag cctgatgccg cagaaaaaga accccgattc gctcga
//
LOCUS       DATIFQ010000106         4197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2140691, whole genome shotgun sequence.
ACCESSION   DATIFQ010000106 DATIFQ010000000
VERSION     DATIFQ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140691"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..3629)
                     /locus_tag="VLE22_04580"
     CDS             complement(24..3629)
                     /locus_tag="VLE22_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB13711.1"
                     /translation="MPEHKSDAELIRDMHNTSRFFVETRHISWVLLIGTALWGIYGYL
                     SMPQRKDPDIPVRIAVALTPWPGASAERVEQLVTSKVEEKIGQNAKVTEIKSISRTGV
                     SIVYLELDQKLKETGKELDDIKLKLDQLQNLPDGAGPIQFLKDFGDTAALMLTVASPK
                     VTEVEVGLRSRSIQSAIEEARRDARGEFAAPRVTLVMGMPQSMNLDVARSSVDLSTRW
                     TRERGLARDIRVLEGPGFLGLDFWTSMTDEEVQQAADRFIHERLQASDFHPDHWDPVV
                     VRDPSETAAKLGAVAAAKYSYRQLDDFTDLIEKTIKTVPQVSKVTRTGLLQEQVHLMY
                     SQERLASYGITPGNLPQILKARNITLPGGILEAQGKNLAIDPSGEFKNEREIGDVLLG
                     ASESGLPLYLRDLVDIMRAYESPARFVNYYTARDSSGHWQRYRAVTLAIQMRQGEQIG
                     RFGEAVDAALNDLRPRLPADLIMARTSDQPLQVRENISLFMTSLYEAIVLVVLVSLIG
                     FWEWRSAVLMALSIPITLAMTYGMMHLLGIDLQQVSIATLIIALGLLVDDPVVAGDAI
                     KRDLAAGHPRLIASWLGPTKLATAILFATITNIVAYLPFLMLSGDTGRFLYSLPIVIT
                     CALVASRIVSMTFIPLLGYYLLRPKAEKPIEERRTTGFASMYYRAGTAAIQHRWKFLF
                     ASFLVLAAGAMIGSRLKTQFFPKDLSYLSFVDVWLSEDASLAATDAVAIQTENIIRRV
                     ADEHGGAGMLKSLTTFTGGGGPRFWFSVSPELQQLNYAQILIEVKDKHFTQHLIGPLQ
                     RAVSSAIPGARIDVRQLETGKTVGLPVQIRVSGDNLVLLRAQAEEIKNILRAIPAADR
                     VRDDWGEENFTVKLRIDPDRARLAGVTNSDVAAAAATGINGYTVATLREGDKQIPVVA
                     RLRNEQRSRLSDIQNLYVYSSRGPEKIPLRQVSSISYEMQTQKTRRLNQFRTITVLCV
                     PVAGVLPSEVLAEAMPRLKQFGGALPPGYKMEIAGEYKDQQESFQELAVVMAISVGMI
                     FLALVIQFRNAVKPFIVFAAIPYGMVGALAALWVMGAPFGFMAFLGIASLVGVIVSHV
                     IVLFDFIEERQAEGHPLVESLLDAGIMRLRPVLITVGATVIALYPLASHGGPLWEPLC
                     YAQIGGLTIATFVTLLLVPVLYAIFVLDLKLVKWTGPPAGHSAGPVISGSISTGA"
     gene            complement(3641..>4197)
                     /locus_tag="VLE22_04585"
     CDS             complement(3641..>4197)
                     /locus_tag="VLE22_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB13712.1"
                     /translation="LSRPIEVGSLVSPGSVGVVLGDTSSVKAAFGVPDAAVAQLHLGQ
                     SLQIRTDAIPGTDFPGRITRIAPAADMKSRLFEVELSVPNRQKQLRPGMIATVTIFGA
                     GSRTPVPVLPLTAIVPSKTERQYAVFILETNGKQHVARMRDVRLGDAVGSSITVLEGV
                     KLGQLVVTSGSNLVTDGQPVQLIP"
BASE COUNT          835 a         1292 c         1299 g          771 t
ORIGIN      
        1 aaaaaagtag acgttgcgcg ccgtcaggct cctgtcgaaa tcgaaccgga gatcaccggc
       61 ccggcgctgt gccctgccgg cggcccggtc cacttcacca gcttcagatc cagaacgaaa
      121 atcgcgtaca gaaccggaac cagcagaagc gtcacgaagg ttgcgatggt cagtccgccg
      181 atctgcgcgt agcacagcgg ctcccacagg ggcccgccgt gggaggcgag cggatacagc
      241 gcgatcaccg tggcgccgac cgtaatcaga accggccgca gccgcatgat acctgcatcg
      301 aggagcgact cgactaacgg atgcccctcc gcctggcgct cttcgatgaa gtcgaacagc
      361 acgattacgt ggctgacgat aacgcccaca aggctggcga tgccgaggaa ggccatgaac
      421 ccaaacggcg ctcccatcac ccacaatgcg gccagtgcgc ccaccatgcc gtacgggatc
      481 gcggcaaaga caatgaacgg cttcacggcg ttcctgaact ggatcaccaa agcgaggaag
      541 atcatcccga ccgagatcgc catgacgaca gccagttcct ggaagctctc ctgctggtcc
      601 ttatactcgc cggcgatctc cattttgtaa ccaggcggga gcgcgccgcc gaactgtttc
      661 agtcgcggca tagcctccgc cagcacttcg gacggtagaa cgccggcgac aggcacgcac
      721 agcaccgtga ttgtgcggaa ctggttcagc ctcctcgtct tctgtgtctg catctcgtag
      781 ctgatgctcg acacctgccg cagcgggatc ttctccggcc cacgggaaga gtacacgtag
      841 agattctgga tgtccgaaag ccgcgaccgt tgctcattgc gcagacgcgc aaccaccggg
      901 atctgcttgt ccccctcgcg gagggttgcg acggtgtagc cattgatgcc ggtagccgcc
      961 gcggcggcaa catcggagtt cgtgacgcct gcaagtctgg ctcggtccgg atcgatgcgg
     1021 agcttgaccg taaaattctc ttctccccag tcgtcacgca cgcgatcagc cgccgggatc
     1081 gcccgcagaa tgttcttgat ctcctctgcc tgtgcgcgca gtagcaccag gttgtcgcct
     1141 gagacacgaa tctgaactgg caagccgacg gtcttgccgg tctcgagctg tctgacgtcg
     1201 attcgcgctc cggggatcgc cgaggacacc gcgcgctgaa gcgggccgat caggtgctgc
     1261 gtgaagtgct tgtctttcac ctcaatgagg atctgggcat agttgagctg ctggagttcg
     1321 ggagaaacgg agaaccagaa cctcgggcca cccccgcctg tgaacgtggt caacgacttg
     1381 agcatgccag cgccgccgtg ctcatcggcc acccggcgaa tgatgttctc ggtctgaatg
     1441 gcgactgcat cggtagccgc cagcgatgcg tcctcggaca accagacgtc gacaaacgac
     1501 agataggaga gatccttggg aaagaactgg gtctttagac gcgatccgat cattgcgccg
     1561 gcagcgagga caaggaagga cgcgaacagg aacttccagc gatgttggat cgccgcggtg
     1621 ccggcgcgat agtacatcga agcgaaaccg gtcgtcctcc gctcctcgat cggtttttcg
     1681 gccttgggcc gcaggaggta ataacctagc aaggggatga aggtcatcga cacgatgcgc
     1741 gaagcgacga gcgcgcaggt gatcacgatc ggcaggctat acaaaaaccg gcccgtgtcg
     1801 ccgctcagca tcaggaacgg caggtacgcg acgatgttcg ttatggtcgc aaacaggatc
     1861 gcggtggcca gcttcgtcgg gccgagccag ctcgcgatca gccgcggatg gccagccgcc
     1921 aagtcacgct tgatggcgtc acccgccacc acggggtcat ccacaaggag tcctagggcg
     1981 atgatgagag tcgcgatcga gacctgctgg aggtcgatcc ccagcaggtg catcatcccg
     2041 taggtcatag ccagcgtgat gggaatggac agggccatca ggacggcgga gcgccattcc
     2101 cagaacccga tcagcgacac gaggaccacc agcacgatag cctcgtaaag actcgtcatg
     2161 aagaggctga tgttctcgcg gacctggagc ggctgatcgg aagtacgagc catgatcaag
     2221 tcggccggca gcctggggcg gagatcgttg agtgccgcat ccaccgcctc gccgaaccgt
     2281 ccgatctgct ctccctggcg catctggatg gctagggtca ccgcgcggta acgctgccaa
     2341 tgcccgctcg agtcgcgcgc cgtgtagtag ttcacgaacc gtgccggact ctcgtaggcg
     2401 cgcataatgt ccacgaggtc gcgcaagtac aatggcaagc cggattccga cgcgccgagt
     2461 aggacgtcgc cgatctcccg ctcgttcttg aactccccgg acgggtcgat ggccaggttc
     2521 ttgccttgcg cttccagaat gcctcccggc agcgtgatgt tccgagcttt gagaatctgt
     2581 ggcagattgc cgggggtgat gccgtaggag gcaagccgct cctgtgagta catgagatga
     2641 acctgctctt gcagcaggcc tgtgcgtgtg accttggaga cttgcggcac ggttttgatc
     2701 gtcttctcga tgagatccgt gaagtcgtcc aactggcggt agctgtattt cgccgccgcg
     2761 accgcgccga gcttggccgc ggtctcggac ggatcgcgca ccactacggg gtcccagtgg
     2821 tcgggatgaa aatcggaagc ctggaggcgc tcgtggatga agcgatcggc agcttgctgg
     2881 acctcttcat cggtcatgct cgtccagaag tccagcccaa gaaaaccagg gccttccagg
     2941 acccggatgt cgcgggcgag gcctcgttcc cgggtccagc gtgtggacag gtcaaccgaa
     3001 ctgcgggcca cgtccaggtt catcgattgc ggcatcccca tcacgagcgt taccctgggc
     3061 gccgcgaatt ctccgcgagc atcacgacgc gcctcttcga tggcgctctg aatgctacgc
     3121 gagcggagcc cgacctcgac ctcggtcact ttcgggctgg caacggtcag catcagggca
     3181 gcagtgtcgc caaagtcctt caggaactga atcggaccgg cgccgtcggg aaggttctga
     3241 agctggtcca atttcagctt gatatcgtcc agctccttgc cggtttcctt cagtttctgg
     3301 tccagctcaa ggtagacgat ggagacccca gtccgcgaaa tggacttgat ctcggtgacc
     3361 ttcgcatttt ggccgatctt ctcctcgacc ttgctggtga caagctgctc gacgcgctca
     3421 gcgcttgctc ccggccacgg cgtaagggcg acggcgatcc ggacgggaat gtccgggtcc
     3481 tttcgctggg gcatgctgag gtagccataa atcccccaca gagccgtgcc aatcagcaac
     3541 acccacgaaa tgtgacgcgt ctcgacgaag aagcgcgatg tgttgtgcat gtcgcgaatg
     3601 agctcggcgt cggacttatg ttcgggcatt gcgatcgcct tcaggggata agctggacag
     3661 gctggccgtc agtgaccagg ttgctgccgc tggtgacgac gagctgcccg agcttgactc
     3721 cttcaaggac cgtgatggaa cttcccacgg cgtctcccag ccggacatcc cgcatgcgcg
     3781 ccacgtgctg cttgccgttg gtttcaagga tgaacacagc gtactggcgc tccgtcttcg
     3841 agggcacgat cgcagtcaac ggcaggaccg gaaccggcgt ccgcgaaccg gcaccgaaga
     3901 tggtcactgt cgcgatcatg cctggccgaa gctgtttctg gcgatttggt accgagagct
     3961 cgacctcgaa caggcgactc ttcatgtcgg ctgccggcgc aattcgagtg atacgccccg
     4021 ggaagtccgt cccgggaatc gcatccgttc gaatttggag agattggccg aggtggagtt
     4081 gcgcaaccgc tgcgtccgga acgccgaagg ctgccttgac ggaactggtg tcgcccagga
     4141 ccacaccgac cgagcccggg ctgaccagcg accccacttc aataggacgg ctgagaa
//
LOCUS       DATIFQ010000107         3894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_34398, whole genome shotgun sequence.
ACCESSION   DATIFQ010000107 DATIFQ010000000
VERSION     DATIFQ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34398"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VLE22_04590"
     CDS             complement(<1..1394)
                     /locus_tag="VLE22_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSB13713.1"
                     /translation="MEKRLILGLVAWTCLASAQPVETLPGTAPLDWQGDLSERMMDGA
                     HRFIERKIAQSRGRRKLDAGSRQRFSERIGVVDPLAAASMERFGDETNPALVAETRSY
                     RVFQVRWTAIEGVHGEGLLLEPKRAPVAFMVALPDVGQTPEQTAVQFAHRFAEAGCSV
                     VVPVLVDRETTFSGHPEIRMTDQPHREWIYRQAYHMGRHIIGYEVQKVRGVVEWFKKR
                     NPHAKVGVAGYAEGGLIAFYSAAVDERIDAAMVSGYFDARERVWAEPIYRNVWGLLDE
                     FGDAEIASLIAPRGLVVEASRPPEWKSVKGDIPAPELASVRTEFERLGSQPKALVEGG
                     PWSAEAMQRFAAMIGVKISAATDKPPSLARPGFDYSARQMRQVTELEAHVQGLVRSSE
                     HVRAASFLYKAEPKFTDERWSTRRRRDTIDPAGFIENAKRYREQFWTELMGRFDDPLL
                     APNPRTRKIFDNAR"
     gene            complement(1493..3634)
                     /locus_tag="VLE22_04595"
     CDS             complement(1493..3634)
                     /locus_tag="VLE22_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSB13714.1"
                     /translation="MMLSLRFTLLAGCFLAVLHGQSDWPVFGHDPGAIRYSPLKQITA
                     GNVSRLRRAWTFHSGKAGSEVTPIVVNGVMYLTQPNGIFALEPETGKLLWKYEAPGAA
                     LRGLCYWPGGRGTHARVFAGVRNNLVAIDVTTGKPAPGFGNEGIVDLKQGVADDLPDA
                     ELVLPSPPAVYRDIVITGSANGEGAPSAGAYGDIRGWDARTGKLVWTFHTVPRPGEPG
                     NETWPEGGWKNRSGTNTWGFFSVDVERGLVFAPTGCPTSDFYGADRHGNNLYANSVIA
                     LDAATGALRWHQQLVHHDLWDYDLAAAPALIEVKRNGKKIPAVAQITKMGTLFIFDRL
                     TGQPLFGIEERPVPPTNIPGEVTSPTQPFPLKPPPLARNSFREEEMYNLTPEHAAFCR
                     DLWRRNGMFVGVPFTPLPLEGNVLIFPSTLGGGNWGGVSFDPSLGYLFANVMNIGQFG
                     HMEKREDPKTGAVTYIRTSEYGPYARFWNRDTRIPCTNPPFGELVAVNVNTGDIAWKV
                     PLGTVESLEAQGVKNTGALNLGGSIATAGGLVFIAATNDSRIRAFESKTGRMLWEHRI
                     DANGHAIPVTYLGKDRKQYVVIMAGGGGGYFGGATSDTLTAFALGAGPSSNEVASSPP
                     RAVPVTEPVSAAPAPRAQGGPVGSPDSAGQAIVDRSCGKQCHSLEVVSSQRLSRTEWK
                     RTVDAMVARGATLTDAEVKLVLDYLSRHFGR"
     gene            3664..>3894
                     /locus_tag="VLE22_04600"
     CDS             3664..>3894
                     /locus_tag="VLE22_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13715.1"
                     /translation="MVPASHRSRPVISSGRSLGLPRTKTTSTNSTAINATVEVSRHGG
                     ASQVCLAPGPVERPFDGRRRVRDLRGGPDCDRR"
BASE COUNT          728 a         1268 c         1160 g          738 t
ORIGIN      
        1 catcgcgcgt tgtcgaaaat cttgcgcgtg cgcggattgg gagccagcag aggatcgtcg
       61 aatcttccca tgagctccgt ccagaactgc tcgcggtagc gcttcgcgtt ttcgataaac
      121 cccgccggat cgatggtatc gcgcctgcgc cgggtggacc accgttcatc ggtgaatttc
      181 ggctccgcct tgtaaaggaa cgacgccgcg cgaacatgtt cggaagagcg gacgagtccc
      241 tggacgtgtg cttctagttc cgtcacctgg cgcatctggc gcgcggagta atcaaaaccc
      301 ggccgcgcga gcgaaggcgg cttgtccgtg gcggcggaaa tcttcactcc gatcatggcg
      361 gcgaaccgtt gcatcgcctc ggccgaccat ggaccgccct cgacgagggc tttcggctga
      421 gagccgagtc gctcgaactc ggtccggacg ctcgccaact cgggcgcggg gatatcgccc
      481 tttacactct tccattcggg cggcctgctg gcttcgacaa ccagcccgcg cggtgcgatc
      541 agagaggcga tctcggcgtc gccgaactcg tcgagcaggc cccagacgtt gcgatagatc
      601 ggctccgccc aaacacgctc ccgcgcgtcg aaatacccgc tgaccatggc ggcgtcgatc
      661 cgttcatcga ctgcggccga gtagaacgcg atcaggccgc cttcggcgta gccggcaacg
      721 ccgaccttcg catgcggatt tcgcttcttg aaccattcca cgaccccgcg caccttctgg
      781 acctcgtacc cgatgatgtg gcgtcccatg tggtaggcct gccggtagat ccattcgcgg
      841 tgcggctggt cggtcatgcg gatctcgggg tgaccggaga atgtcgtctc gcgatcaacc
      901 agcaccggca caaccacgga gcagccggct tcggcgaagc gatgagcgaa ctgcacggcc
      961 gtctgttccg gcgtctggcc gacgtccgga agcgcaacca tgaaggccac gggcgcccgc
     1021 tttggttcca gcagcagccc ttctccgtgc acgccttcga ttgccgtcca gcggacctgg
     1081 aaaacgcgat acgaccttgt ttcggccacc agagccggat tcgtttcgtc gccgaagcgt
     1141 tccatcgagg cagccgcgag cgggtccacg acgccgatcc gctcgctgaa acgctgtcgg
     1201 ctcccagcgt ccagcttccg ccgcccgcgc gactgcgcga tcttccgctc gatgaagcgg
     1261 tgcgcgccgt ccatcatccg ctccgacagg tcgccttgcc agtcgagcgg agcggtgcca
     1321 ggcaaggttt ccaccggctg ggcagaagca aggcaagtcc aggctaccaa cccgagaatc
     1381 agtcgttttt ccatgagtta ctcccggcag tgcagagcat gagagagggc cagcgtatca
     1441 gccgcttgtc cccactagtg tcaccttcat cagatccgcc agcgcgatgc cattatcggc
     1501 caaaatggcg agacaggtaa tccaaaacca atttcacttc tgcatctgtt agcgtcgccc
     1561 ctcgggccac catggcatcc accgttcgct tccactccgt cctcgacaag cgctgactgc
     1621 ttacgacttc aagactgtga cactgtttgc cgcacgaccg gtccactatg gcttggcccg
     1681 ctgaatcggg cgagcccacg ggaccgccct gcgcacgagg cgctggcgca gcagacactg
     1741 gctccgtgac gggaactgca cggggcggcg aggaagccac ctcatttgag gaagggccgg
     1801 cgcccaaggc gaatgctgta agagtgtcgc tggtggcacc tccgaaataa ccgccgcctc
     1861 ctccggccat aatcacgacg tactgtttcc gatccttacc gaggtaggtg accggtattg
     1921 cgtgcccgtt agcatcgata cgatgctccc agagcatccg gccggtcttc gactcgaacg
     1981 cgcgaatgcg gctgtcgttg gtggctgcaa tgaaaaccag tccgcccgcg gtcgcgatac
     2041 tcccacccag gtttagcgct ccggtattct tgactccttg cgcttcgagg gattccacag
     2101 ttccgagcgg taccttccag gcgatgtccc cggtgttgac gttgaccgcc acaagttcgc
     2161 cgaacggagg attcgtgcac gggatgcggg tatcgcggtt ccagaaccgt gcgtacgggc
     2221 cgtattcgga ggtgcggatg taagtgaccg cgccggtctt cgggtcttcc cgtttctcca
     2281 tatgcccgaa ctgtccgatg ttcataacgt tcgcgaagag atagccgagg gacgggtcga
     2341 aagacacgcc gccccagtta ccgccgccca aagtactcgg gaagatgaga acgttaccct
     2401 ccagcggcaa cggtgtgaac ggcacgccga cgaacatgcc gttcctgcgc cacaggtccc
     2461 tgcaaaatgc cgcatgctcg ggtgtcaggt tgtacatctc ctcttcacga aaagaattgc
     2521 gcgccagcgg aggcggctta agcgggaacg gttgtgtggg cgaggtcacc tcgccgggga
     2581 tgttcgtcgg cggcaccgga cgctcttcaa ttccgaacaa cggttgtccg gtaaggcggt
     2641 cgaagatgaa tagcgttccc atcttcgtga tttgcgccac cgctggaatc ttcttcccat
     2701 tccgcttgac ttcgatcagc gccggcgccg ccgccagatc gtaatcccag agatcatgat
     2761 gaaccagttg ctggtgccac ctcagggctc cggtagctgc atctagggcg attacgctgt
     2821 tggcgtaaag gttatttccg tggcggtctg cgccataaaa atcggatgtg ggacagccgg
     2881 tcggggcaaa aaccagccca cgctcgacat ccaccgaaaa gaatccccac gtatttgtgc
     2941 ccgaccggtt cttccagcca ccctccggcc acgtctcatt gcccggttca cctggccgcg
     3001 gcactgtatg aaaggtccac accagtttcc ccgtgcgcgc atcccaaccg cggatgtcac
     3061 cgtaagcgcc agcgctggga gcaccctcac cgttggcact accggtgatc acgatgtccc
     3121 ggtacaccgc cggaggagac ggcaacacca gttcggcatc aggcaggtca tcggcaacac
     3181 cctgcttcaa atccacaatc ccttcgttgc cgaagcccgg cgctggcttg cccgtcgtca
     3241 cgtcgatcgc aacaagattg tttcggaccc ccgcaaagac acgggcatgt gtccctctgc
     3301 cgccgggcca gtagcacaga ccgcgaagcg ccgcgcccgg ggcttcatat ttccagagca
     3361 gtttgccggt ctccggctcc agcgcgaaaa tgccgttcgg ctgggtcagg tacatgacgc
     3421 cgttaaccac aatgggcgtg acttcggaac cggctttgcc gctgtgaaag gtccaggctc
     3481 gccgcaagcg ggaaacgttt ccggccgtga tctgcttcag cggcgagtaa cgtatggcgc
     3541 cagggtcgtg gccgaagacc ggccagtcgc tttgcccgtg caggacggcc aggaaacacc
     3601 ctgctaagag tgtgaagcgg agagataaca tcatgtgccg aaccccagaa cggatgaatt
     3661 cacatggtac ccgcaagcca tcgctcgcgc ccggtgatct cttctggccg gtcgctgggt
     3721 ctcccgcgga cgaagacgac ctccacgaat tctacagcaa tcaacgccac cgtcgaggtg
     3781 agccgccacg gcggcgcttc tcaagtgtgt ctagcgccgg gcccggtcga gcgcccgttt
     3841 gacggccggc gtcgggttcg tgatctccgc ggcggaccgg attgtgatcg tcgg
//
LOCUS       DATIFQ010000108         3486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2525335, whole genome shotgun sequence.
ACCESSION   DATIFQ010000108 DATIFQ010000000
VERSION     DATIFQ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2525335"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..708)
                     /gene="rplJ"
                     /locus_tag="VLE22_04605"
     CDS             complement(181..708)
                     /gene="rplJ"
                     /locus_tag="VLE22_04605"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HSB13716.1"
                     /translation="MKKREDKRKEVESLRKELERVDHLFVAGFEKLKVGQDFQLRKIV
                     RSAGGRYKVIKNNLAAKAAEGTKAEDLMKGLAGMTSLAYTAADPVALAKVLTAYAKEN
                     PTFQFKAGMVEGRVIDVRTVGELASLPSREEMFGKLLYLIQTPAQRLVSAINGVGRKL
                     AVVVDQACQENKFTA"
     gene            complement(728..1435)
                     /gene="rplA"
                     /locus_tag="VLE22_04610"
     CDS             complement(728..1435)
                     /gene="rplA"
                     /locus_tag="VLE22_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HSB13717.1"
                     /translation="MARKQGKKYASAAQQIERRPYTLEEAVPLVQRLKFAKFDETVEL
                     HMRLGVDPKHADQMVRGTIVLPNGLGKSKKVLVIASGDKQKEAEEAGADFVGGEDMVA
                     KIQQENWTDFDAVVATPDMMRSVGRLGKVLGPRGLMPNPKTGTVTVDVARAVKEVKAG
                     KVEFRVDKTGSIHVPVGKASFAGDKLLENTQTLIQAILKAKPSAAKGKYVRSATVCST
                     MGPGVSLDVTPFNTKIV"
     gene            complement(1562..1990)
                     /gene="rplK"
                     /locus_tag="VLE22_04615"
     CDS             complement(1562..1990)
                     /gene="rplK"
                     /locus_tag="VLE22_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSB13718.1"
                     /translation="MAKKVTGQVKLQIPAGKATPAPPVGTALGPQGVNIMEFCKAFNA
                     KTSGKDQDGLIIPVVITIYNDRSFTFITKTPPVPVLIKRAVNLAKGSAEPNKNKVGRI
                     TEKQVEEIAKIKMPDLNCFTLEAAMQSVKGTARSMGVDVV"
     gene            complement(2067..2780)
                     /gene="nusG"
                     /locus_tag="VLE22_04620"
     CDS             complement(2067..2780)
                     /gene="nusG"
                     /locus_tag="VLE22_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HSB13719.1"
                     /translation="MEEKDLENLGEEDTGVKAPEPGAEPLPEEPETKGASSGESSDVA
                     GAEASAQIPPEAAVSEPSSKQWYILHTYSGFEQKVADSLRGRGEAFGFADKIGQVLIP
                     TEEVVELRGGKKVTSKRLLYPGYVLVEMEMNDELWHAVKSTPRVTGFVGGGNTPVPLT
                     ADEVNQILYRQSSSAEKPRPKMSFEKSEMVRIVDGPFANFSGKVDEVNTERNTLRVLV
                     TIFGRATPVELDFLQVEKI"
     gene            complement(2796..3014)
                     /gene="secE"
                     /locus_tag="VLE22_04625"
     CDS             complement(2796..3014)
                     /gene="secE"
                     /locus_tag="VLE22_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HSB13720.1"
                     /translation="MVQRVSGWPVAIKEYTQELQLEMRRVTWPAWKQVRATTGVVIAC
                     VFVFAAYFWIVDFVLGRSVGKVFETFSK"
     gene            complement(3076..3150)
                     /locus_tag="VLE22_04630"
     tRNA            complement(3076..3150)
                     /locus_tag="VLE22_04630"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3115..3117),aa:Trp,seq:cca)
BASE COUNT          611 a         1145 c          925 g          805 t
ORIGIN      
        1 gcttctcttc gagcaacttc acgagctgag atgcctcgag cagcgtcagc ccctggattt
       61 gttccgcaat cgcgttgata tccgccatgt tctcttcctt gaactccttc tgcctgcagc
      121 ttgtagcctg cgacccgtgc aaacacggcc cgaaaaatcc ctgaccacgg gtcaccggcc
      181 ctacgcagta aacttgttct cctggcacgc ctggtcgacg accaccgcca gtttgcgccc
      241 gactccgttg atcgccgaaa ccagccgctg tgccggagtc tggatcagat aaagcagctt
      301 cccgaacatc tcctcgcgag agggcagcga cgccagttcc ccgacggtcc ggacgtcgat
      361 tacccgtcct tccaccatgc ccgccttgaa ctggaacgtc gggttttctt tcgcgtaggc
      421 tgtgagcacc ttcgccagcg ccaccgggtc cgccgctgtg taagcgagcg acgtcattcc
      481 ggcgaggccc ttcatgaggt cctccgcctt cgtcccctcg gcggctttcg ccgcgaggtt
      541 gttctttatg accttgtacc ggcctcccgc ggaccggacg attttgcgaa gctggaagtc
      601 ctggccgacc ttcagttttt caaagccggc cacgaacagg tgatccaccc gctccagttc
      661 cttacgcagc gactcgactt ccttccgctt gtcttctctc ttcttcatgg gtttcccctg
      721 ctagcggcta aacgatcttc gtattgaacg gcgtcacatc cagtgaaaca ccgggtccca
      781 tcgttgagca gacggttgcg ctccgcacgt acttgccttt cgcggctgac ggcttcgcct
      841 tcaggatggc ctgaatcaac gtctgggtat tctccagcag cttgtcaccg gcgaaactgg
      901 ccttaccgac gggcacgtgt atggagccgg tcttgtcgac ccggaactca accttacctg
      961 ccttcacttc cttaacggct cgggcgacat ccaccgttac cgtgccggtc tttgggttgg
     1021 gcatgaggcc acgggggccg agcacttttc cgagccgtcc cacggaccgc atcatgtccg
     1081 gggtggcgac gacggcgtca aagtcagtcc agttctcctg ctggatcttc gcgaccatgt
     1141 cttcgccgcc cacgaagtcg gcgccagcct cttccgcttc cttctgcttg tcgccagagg
     1201 cgatcaccag aacttttttg gattttccca aaccgttggg cagaacgatg gtgccacgca
     1261 ccatctggtc ggcgtgcttc ggatcgacgc cgagacgcat gtggagttcg acagtctcgt
     1321 cgaatttggc gaacttgagc cgctggacaa gaggtaccgc ctcttccagg gtgtagggtc
     1381 tgcgctcaat ctgttgagcg gcggatgcgt acttctttcc ttgtttgcga gccattgttc
     1441 tcctggtcct aacggcagga ttcgttcaga cccctgcctc ccatggtttc tacaacacga
     1501 attttcaggc ggagcttgaa gtatagcaga ccaaaggtag gcccttcaac cggaacccct
     1561 cctagacgac gtccactccc atgctgcgag cggtgccctt gacggactgc atcgcggcct
     1621 cgagcgtgaa gcagttcagg tccggcatct tgatcttcgc gatttcctcc acctgcttct
     1681 cggtgatccg gcccacctta ttcttgttcg gttccgcgga ccccttggct aggttcaccg
     1741 cgcgcttgat cagcaccggc acgggcggag tcttggtgat gaaggtgaag gagcggtcgt
     1801 tgtaaatcgt gatcacgacc gggatgatca acccgtcctg gtctttgcct gacgttttcg
     1861 cattgaacgc cttgcagaac tccataatgt tgacgccctg cggaccgagc gccgtaccaa
     1921 ccggcggagc gggagtcgcc ttcccggccg gaatttgcag cttaacctgt ccagtaacct
     1981 tcttcgccat tattcaacac ctttcaccgc acggtgcgcc gaggcggact cttaattccg
     2041 taatccgtaa gcgccccgtg gttcgtctaa atcttctcca cctgcagaaa gtccagttcg
     2101 accggcgtcg cgcgaccgaa gatcgtcacc agaacgcgca gtgtgtttcg ctcggtgttc
     2161 acctcgtcca ccttgcccga gaagtttgcg aaggggccgt cgacgatcct caccatctcg
     2221 ctcttctcga aagacatttt cggacgcggt ttctcggccg aggaggactg ccgatacagg
     2281 atctggttca cctcgtccgc cgtgagaggc acgggcgtgt tcccaccgcc gacgaaaccg
     2341 gtgacgcgcg gcgtcgactt cacagcgtgc catagctcgt cgttcatttc catctcaacg
     2401 agcacgtagc ccggataaag cagccgctta ctggtcactt tcttgccgcc gcggagttcc
     2461 acaacctcct cggtcggaat caggacctgc ccgatcttgt cagcgaaccc gaacgcctcg
     2521 ccgcggcccc gcagggagtc ggcgaccttc tgctcaaaac ccgaataggt gtggaggata
     2581 taccactgtt tggaagaggg ctcgctcact gccgcttccg ggggaatctg agccgatgct
     2641 tccgcgccgg caacgtcgga ggactcgccg gaggaggcgc ctttcgtttc cggttcctcg
     2701 gggaggggct ccgcaccggg ctccggcgct ttcacgccgg tatcctcttc cccgagattc
     2761 tcgagatctt tctcttccac ggtctgctcc ctgcactact tgctgaacgt ctcgaagacc
     2821 tttccaacgg accggcccaa gacaaagtct acgatccaga agtaggccgc aaacacaaac
     2881 acgcacgcga ttacgacccc ggtcgtcgcc cgcacctgct tccatgccgg ccacgtcaca
     2941 cggcgcatct cgagctgtag ttcctgggtg tattccttga tcgcgacagg ccagcccgaa
     3001 acgcgctgaa ccaagctctt gctctcttcc atagttttca atttgccgtg gctgttgagc
     3061 gacgggaatg ttctctggca ggggcggagg gattcgaacc cccactcgcg gttttggaga
     3121 ccgctggtct accgttaaac ctacgcccct gcgtgaaggg ccgaagcacg cctcgcccct
     3181 tttattttat ctcacggtgc acctgatgct tgcgacaatg gggacagaac ttctttagct
     3241 cgatccgctc cgtaaccgtc ttcttgttct tggtacttgt atagttccga ttcttgcact
     3301 cagtgcactg gaaggtgatg atctcgcgcg gcattgtcga ttccttactc caagacttcc
     3361 gtaaccgtac cggcgccgac ggtgcgcccg ccctcgcgga tcgcaaaccg caagcccttg
     3421 tccatcgcca ccggcgtgat caactccacc gtcaggttca cgttgtcccc gggcatcacc
     3481 atctcc
//
LOCUS       DATIFQ010000109        11174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1951348, whole genome shotgun sequence.
ACCESSION   DATIFQ010000109 DATIFQ010000000
VERSION     DATIFQ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11174
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951348"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /locus_tag="VLE22_04635"
     CDS             <1..1620
                     /locus_tag="VLE22_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB13721.1"
                     /translation="DADDKWLPQRLERQVADLESHPDAAMVYGPTRYWFSWTGKPEDR
                     QRDFVAPLGLPPNTLVKPPALFTTCHPLGEAPSPATCSLTLRRSLLETTGGFEDSFRD
                     SYEDRAFLVKVYLHGNVFVSGDCLANYRIHPDSHSFRALEARQSNRERRIFLKWLEGY
                     LKAQRIQDKAIWRALGHARRRWRYTVLTKWFESGRRYSTRARYKWLRVKRWVGRRTAY
                     IEARPNPIPVSAATADRFPIGATTLSWTAGTETEVEVHVDSPDGPLLSRTAGCGTITT
                     GPWVHDSMPFFLQDVSNGKPLTAENTLDVTVVRVTKPRLRLTPGLGKTRFGDLRRLTP
                     ISSHWGFDRGLPVDRHYIEGFLARYAGDIRGRVLEVGGSAYTRRFGSGLVTQSDVLNL
                     LDGVPGTTVVADLACAPQIPSEAFDCVIVTQTLQCIYEVRSAVQTLWRILKPGGVLLA
                     TIPGISQTYDPEWGEYWCWNFTVISARRLFEEAFSTTKVQIDSFGNAFTAICFLEGIA
                     AEELRAEELDHHDPGYVVTIAVRAVKSEERP"
     gene            1617..5174
                     /locus_tag="VLE22_04640"
     CDS             1617..5174
                     /locus_tag="VLE22_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB13722.1"
                     /translation="MKATGTLTAHPNPVLADASGMGATTLSWSSEGTDDVEVRVGAPD
                     GPLLSRTAGSGSTTTGKWVGDGMVFYLQDVSDRAPLTSEHVLATVTVRHDSQIDRSGP
                     KALILLYHRVAEVQPDPWSLCVTPQHFAEHLDVLSSRWNVLRLRDLVRALQDGALPDR
                     SIAITFDDGYADNLHNAKPILERYGKPATTFVSSGYVGNKREFWWDELERIVRRDGSL
                     NLDLYLSLHRLLQPLTHTERCRMLDGFLEWEGIEPGVRETHSTLSATELVELARGDLI
                     EIGSHTFTHALLDALPEAQQRAEIVQSKAGLEEILGGRVDHFAYPYGHYNEATISILR
                     RAGFACACTTNASSVDKYSDRFQLPRLTVGDWGGEEFARRLSDWYGSQTRKAFEAVQA
                     ADSQAEMAPAPAKRRAEVQDSTPDRQLERAERVRLQVEAGLRAFQAQFDSVLRAYRAQ
                     RAWQAMLILRKAYTLLVQKRDVTSFLMWVLRFPLKSTNDLLESELSFPEVQSYLPPEL
                     KSTFRASSTQPVNSGAAARRVPTQTKYDVVVLPVFEFDFRFQRPQQLAAQFARNGHRV
                     FWVSPSRRIPGSATDAYELLTLRENLWEVHPRGGSPSIYAGSLSSENHADVLASLRNL
                     YRHWAIAESVVLIELPFWRSIGLSLREMFGASVVFDCMDDWQSMPMISEFNRREERVL
                     VTECDLLVLSARQLSEKYTASARRSVLASNAADFGAFSAPVDAHDSLAGVPKPVVGYF
                     GAIADWFNYDLFHDVVTSRPQYSFVLVGGLGLEKEVCGEEIARFRELPNVFLVGHRPY
                     SELPSYLRQFDACVIPFVISDVTRATDPVKVYEYLSQGKPIVATAMPELSDRSSLIYL
                     AEDAADFARKLDLALSENDPELKRRRSSYASRNTWRDRLETIDAAVRETFPRVSILIV
                     TYNSQDYIQPCLESVIRNTSYPSYEILIVDNGSSDGTVQLLKDYAEAERRIRVIALPD
                     NKGFAAANNTASRLAHGEYLIFLNIDTIVTPGWVGRLVRHHQNDPSVGVVVPVTNWAG
                     NEARINTDYANYAEMEEFAIELAQKNMGMSNELDVAPFFCALIPREVWTQAGELDERF
                     EIGMFEDDDYSIRVRQLGRRIITAEDCFIHHFGRGSFSKMSSTEYRRIFDSNLRRFEE
                     KWAKPWIQHKFRPGIRPEHVQHNPGDFNTALPSRQGFQR"
     gene            6179..8437
                     /locus_tag="VLE22_04645"
     CDS             6179..8437
                     /locus_tag="VLE22_04645"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSB13723.1"
                     /translation="MKPLASLFLLLAAGTPGSQAVQDYFPMAVWYGGGKARAPMLERD
                     PHAKKETWRKDLQQIKSLGFNTIRCWIDWASGEPSEGDYRFDTVDVLLELAEQEGLKL
                     VIQVYMDAAPDWVGKRYPDSLFVAAGGEVMRPEPAPGYCFDHAGVQKATLGFFTALAR
                     RAAKSPAFLGWDLWSEPHVINWAMAPYLPNAEFCFCPHSVGRFRSWLKKKYGTLDALN
                     VAWYRRFSDWNQVEPGRLSTILSYTDYIDWRTFIQEKLGEDLRMRYEAVKRGAPDRVA
                     TSHAAVPSLFTSPLAGDGAPDDWIMARQADYYGTSFYPKHSSPVGRDVPWRAALMDFA
                     RSSAKDRGFWVGELQGGFGTIALRVSATVTPDDLRVWTWTALSRGAKGLNFYAWYPMS
                     SGYESGGYGLIQLDGTITERSRAAGEIARVVDRNQKLFLDARPPKAEVAIVYNPMVHF
                     VGGRQRAFSSPAAQGEVFGIERDSLLGTYRALFPTNVPVDFVHINEITVAELKPYKLV
                     IFPYPLMMPGRVATELAGYVRAGGALVSEARLGWNNEQGWAAETIPGLGLHEIMGCRE
                     TAIQTTPNNRTELTWTGDGIPGLKPGDRLPGRLYEETLEPLGAQSKAVAKHANGAPAA
                     VISSFGAGKTLTLGTYVGVAYEAQQDPAVQRLFTGLLEWAGVTRPVESSAADTEVRWL
                     ESGSDRIVFIFHHADKPSDVPVALSMPPGNWTAVDLQSGRVVELNRSGASVRFGKRLG
                     PSEVWVLRLSPE"
     gene            8527..8979
                     /locus_tag="VLE22_04650"
     CDS             8527..8979
                     /locus_tag="VLE22_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13724.1"
                     /translation="MLSRTLVQMIEDHCEQITDRLLRHQRQDPELIQVKRLPESELRD
                     RAGEVLKNLGHWLSAGREAELSSRYELLGHRRFEEAIPLYEVVRSLHLLKTNMIEYVR
                     EQGIGQNPVELYAEEELEHRVGLFFDGAVYHTVHGYETALRKKAHLAA"
     gene            9035..10504
                     /locus_tag="VLE22_04655"
     CDS             9035..10504
                     /locus_tag="VLE22_04655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HSB13725.1"
                     /translation="MTVGPQARRRNIEDLRTGSLDVLVIGGGINGVGVARDLALRSDH
                     AGAGLRIGLVEQHHFASGTSGKNSQLIHGGLRYLKYFQFGMVRESLRERALLLRLAPH
                     LVEPLPLLIPMFSSFDRFYYGAGLCLYDLLAGSRNIGKHRRLSLPAVAGLEPGLSHER
                     LSGGAIFFDCRVHSARLVLENVLDAARLGTIIANYVLAVDLRRESGRWRVSIEDAFSG
                     ERFEATAREIVDAAGPWSTGAPLRLVRGSHLILPRITTGERAIAHFDENGRIVFLIPW
                     GSHKQLTLVGTTDVDHESGPDDVEISPEERAYLLRIVRKLFPGAAGVEPVSTFSSLRP
                     LLRDGSGSPVAASREYRIWRSDDGIVRIAGGKYTTYRSMSEQAADLVLRNIAPELARM
                     HVTAEAPFPPPEGRRIEDLPVDEQASLVVNCEMAQRLPDLLFVSTYLGYERIWDRAAL
                     RPFAEAMALHLGWDERRMHEEIDLVLRLCGRPPASTLRT"
     gene            10501..>11174
                     /locus_tag="VLE22_04660"
     CDS             10501..>11174
                     /locus_tag="VLE22_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSB13726.1"
                     /translation="MSPYWRTFWWLFRRSFVLAFQDSCFGVAKAAAYSALLSLFPLLT
                     ATATILVQVRAEPITRVISRALSQVLPPGTEDLVLNHFRVHGERPSGLLVAATLISIW
                     AASRVMTSLMEGFQAAYHVPVDRPFLRQQGMAILLVFATAVPVVAASGLILFGTRIER
                     AVVAWLGLVPAGADLRSGVAFTSSVLRFAVALGSMILVNAMMYRLGPNRPQRWRNVWP
                     GAIVATV"
BASE COUNT         2109 a         3411 c         3419 g         2235 t
ORIGIN      
        1 gacgcagacg acaagtggct accccaaagg ctggagcggc aggtggcgga tctggagtcg
       61 caccctgacg ctgccatggt ctatggcccc acccgatact ggtttagctg gaccggcaag
      121 ccggaggatc gccaacgaga ctttgtggct ccgctcgggc tgccgcccaa cacgctggtt
      181 aagccaccgg ctctcttcac gacttgtcac ccgctgggag aggcgccgtc tcccgccact
      241 tgcagcctca cgcttcgccg ttcccttctc gagacgaccg gcgggttcga ggactctttc
      301 cgagattcgt acgaggatcg agcattcctg gtaaaagtct accttcacgg gaacgtattc
      361 gtttccggag actgcctggc gaactaccgc atacatccag attcgcattc gttccgcgcg
      421 ctagaagcta ggcaatccaa ccgtgaacgt cggatctttc tgaagtggtt ggaaggctac
      481 ctgaaagctc agcggatcca ggacaaggcg atctggcggg cgcttggcca cgcgcgtcga
      541 cgctggcgct atacggtcct gaccaagtgg tttgaatccg gcaggcggta ttcgacaaga
      601 gcacgttaca agtggctcag agttaagcga tgggtcggac ggcgaaccgc atacatcgag
      661 gcccgtccca atccgattcc ggtgtccgcc gccaccgcgg accgtttccc gattggtgcg
      721 acgacattgt catggaccgc cgggacggaa acggaggttg aggtacatgt ggactcgccg
      781 gacggaccgc ttctgagtcg cacggcgggc tgcggaacaa tcactacggg cccctgggtt
      841 cacgatagta tgccgttctt cctgcaggat gtgtccaatg ggaagccgct cacggcggaa
      901 aacacccttg acgtcaccgt cgtgagggtt accaagccac gactgcggct tactccgggt
      961 ttaggcaaaa cacgctttgg cgatcttagg cgactgacgc cgatcagcag tcattggggc
     1021 tttgaccgag ggcttccggt cgatcgtcac tatatcgagg gattcctcgc ccgctacgct
     1081 ggagatattc gtgggcgtgt gctggaggtc ggcggttccg cttatacgcg gcgtttcggc
     1141 tccggcctcg tcacacagag cgacgtcttg aacctgctgg acggcgttcc cgggacgacg
     1201 gttgtagctg atcttgcgtg cgcgccccaa atcccgtccg aagccttcga ctgtgtcatc
     1261 gtcacgcaaa ccctccagtg tatttacgag gtccgttcgg ccgtccagac tctttggcgg
     1321 atcttgaaac cgggcggcgt ccttctggcc actatccctg gcatcagcca gacatacgat
     1381 ccggagtggg gagagtactg gtgctggaat ttcaccgtca tttcagcccg gcgtttattc
     1441 gaggaagcgt tctcgaccac gaaggtccag attgacagct tcggaaatgc ctttacagca
     1501 atatgctttc tcgagggcat cgccgcagaa gaattgcggg ccgaggaatt ggatcatcat
     1561 gatccaggct atgtggtcac tattgcggtt cgcgccgtga aatcggaaga acgaccatga
     1621 aggccacagg caccctcaca gcgcatccca atcccgtttt ggcggacgcc tcaggaatgg
     1681 gggcgaccac tttgtcgtgg agctccgagg gaaccgatga cgttgaagtg cgcgtgggcg
     1741 ccccggatgg ccccctgttg agccgcactg caggttccgg gagtacaacc actggcaaat
     1801 gggtgggcga tgggatggtc ttctatcttc aggacgtatc cgatcgcgcg ccgctgactt
     1861 ccgagcatgt gttggccacg gtcacggtgc gccatgatag ccagatcgac aggtccggcc
     1921 caaaggctct catcctcctg tatcaccggg tggcggaggt gcaaccggat ccgtggtcct
     1981 tgtgtgtcac accgcaacac ttcgctgaac acctggacgt tctgtccagc cgatggaacg
     2041 tattgcggct ccgggatttg gtgcgtgcgc tgcaagacgg cgcacttccg gaccggtcga
     2101 tcgccattac ctttgacgac ggctatgccg acaatctgca taacgccaaa ccaatactgg
     2161 aacgttacgg aaagcccgca accaccttcg tctcgagcgg ctacgtcggc aacaaacggg
     2221 agttctggtg ggacgaactg gaacgaatcg tgcggcgcga cggcagtctg aacctggatc
     2281 tttacctatc tctccaccgt cttttgcagc ccctgacaca cactgagcgg tgccggatgc
     2341 tggatggatt cctggagtgg gaagggatag agccgggagt tcgcgagacc catagcacgc
     2401 taagcgccac ggagctcgtt gaactggcaa ggggcgatct gatcgagatt ggctcacaca
     2461 cgttcactca cgcgctcctt gatgctttgc cggaggcgca gcaacgtgcc gagatcgtgc
     2521 aaagcaaggc cgggctggag gaaatcttgg gcgggcgcgt ggatcatttc gcctatcctt
     2581 acggccacta taacgaggcg accatcagta ttcttcggcg cgccggcttc gcctgtgcgt
     2641 gcacaacaaa tgccagttcc gtggacaagt actcggaccg gttccaactg ccccgactca
     2701 cggtaggaga ctggggaggt gaagagtttg cacggcgcct ctcagactgg tatggctcac
     2761 agacccggaa agcattcgag gcggtgcagg ccgctgattc ccaggcggag atggcgcctg
     2821 cgcctgcgaa acggcgcgcc gaagtccagg actcaacacc agaccggcaa ttagagaggg
     2881 ccgagcgcgt tcggctgcag gtggaagcgg gattgcgggc atttcaggct caattcgatt
     2941 ctgtcctcag ggcctatcga gctcagcgag cctggcaggc gatgcttatt ttgcggaagg
     3001 cttacacgct tctcgtccaa aaaagggatg tgacctcatt tctcatgtgg gtgttgcggt
     3061 tccctctgaa atcaacaaac gatcttcttg agtccgagct gagttttcct gaagtccaga
     3121 gttacctccc gccggagctg aaatccacct tccgtgcctc ttccacccag ccagttaact
     3181 ccggcgccgc ggcacggaga gttcctactc agaccaagta cgacgtcgtc gtccttccgg
     3241 tgttcgagtt tgatttccgc ttccagcggc cccagcaact tgcggctcag ttcgcccgca
     3301 atggtcaccg ggtgttttgg gtgagcccgt ccagacgaat cccggggtct gctaccgatg
     3361 cttatgaact actgacgcta cgggaaaacc tctgggaggt gcatccgcgc ggcggcagtc
     3421 cgagcattta cgccggcagt ttgtccagcg aaaaccatgc ggacgtactg gcatccctga
     3481 gaaacctgta ccggcactgg gcgattgccg aaagtgtagt cctaatcgag ttgccgttct
     3541 ggcgaagcat cggcttgagt cttcgcgaga tgtttggggc gagtgtcgta ttcgactgta
     3601 tggacgactg gcagtctatg ccgatgatca gtgagttcaa ccgccgtgaa gagcgcgtgt
     3661 tggtcacgga atgcgacctc ctcgtgctct cagcccggca attgtccgaa aagtacaccg
     3721 cctccgcacg ccgctccgta ctggcttcca acgccgccga ttttggcgcc ttcagcgccc
     3781 ctgtggatgc acatgacagc ctggccggcg tgccgaagcc ggttgttggg tactttggag
     3841 cgatcgccga ttggttcaat tacgatctct tccacgatgt ggtcacgtcg cgacctcagt
     3901 attcttttgt gctggtagga ggtctgggac tggaaaaaga ggtgtgtgga gaagaaatcg
     3961 cgcgatttcg cgagttacca aacgtttttc tcgtgggtca tagaccttac agcgaacttc
     4021 cttcctatct ccgccaattt gatgcgtgcg tcataccttt cgtgatcagt gacgtgaccc
     4081 gcgccacaga tcccgtgaag gtgtatgaat atctgagtca gggaaagccc attgtcgcca
     4141 cagctatgcc tgagctgtcg gaccggtcga gtttgatcta tcttgccgag gatgccgccg
     4201 acttcgcccg caagcttgat ctcgccctct ccgaaaatga cccggaattg aaacgccgcc
     4261 ggtcgtccta cgcctcacgg aatacctggc gcgaccggct ggaaaccatt gacgccgccg
     4321 tgcgagagac ctttccacgg gtttcaatcc tgatcgtgac ttataacagc caggattaca
     4381 tccaaccctg tctggaatcg gtaatccgca acacctccta tccgagctac gagatcctga
     4441 ttgtggacaa cggatcttca gacggcacgg tgcagttgct gaaggattac gccgaggccg
     4501 agcgccgcat tcgcgtgatc gcccttcctg acaacaaggg gtttgcggct gcgaacaata
     4561 ctgcatcccg gctggcccac ggcgagtacc tgatctttct caacatagac acgatcgtga
     4621 cccctggctg ggtgggacgg ctcgtccggc atcatcagaa cgatccatcg gtgggtgtgg
     4681 tcgtgccggt aactaactgg gccggcaacg aggcgagaat caacaccgac tatgccaact
     4741 atgccgagat ggaggagttc gccatagaac tcgctcagaa gaatatgggc atgtcaaacg
     4801 aacttgacgt ggctcccttc ttctgtgcgc tgattccccg tgaggtgtgg actcaagccg
     4861 gagaattgga tgagcggttt gagatcggga tgttcgagga cgacgactac tcaatccggg
     4921 tacgtcagct tggccgccgg attattacgg cggaggactg tttcatacac cattttggcc
     4981 gcggatcctt ctccaagatg agttcaacgg agtaccggag gatttttgac agcaacctcc
     5041 ggcgcttcga agaaaagtgg gcgaagccgt ggattcagca caagttccgt cccggtatcc
     5101 gcccggaaca tgtgcaacat aacccgggcg acttcaacac agcgcttcca agccggcagg
     5161 gcttccagcg ctagcctggc gggggtccgg ttggtcgtgc aatcgttccc ccagtcgtcc
     5221 tagaccttcc gtgcggcggt ccagaactag gtcctacatc cgtgcgcttc ttgcggcaga
     5281 gggaaccagg acaacaaggg tcgcttgcgt gaacgcagcc tccaggcgtt gggaagcgcg
     5341 gacagtgccc acgaggtaca ggttccgatg gaggcctatt ctcccgctgt gtggaggcca
     5401 gtaagatcgc ccctaggagg gtttcaatga aaacgcttgt attgtttggc ttgtgtgtac
     5461 tgacggtgaa cgccgccgag tttgcacagg caaaggcgag tgcggtctca ggaggcgggc
     5521 ttttggtctc gtggcgggag acaggactgt ctccggaatc tccatactcg tatgaagtca
     5581 cggccgacgc tacggcgaac tactggtgca tcgataccgg ggcgagacac cccaccgcaa
     5641 agagcacatg gccggtgatt tcaccagacc ctaaatccgg tgggacgttt acgtcatcca
     5701 agtcgggaaa cgtgtcaggg gaactcccat tatccccacc acccggcccg acgggagtgt
     5761 gtaatgctgg ggagtctcta gttctccatg acgtgactta cgaggcagtc gagttaacgg
     5821 atgggaccat ggttgtgtcc atcgatgggg tgtacagtgt cctcctgttt gggaggaccg
     5881 tcggggccgg gaagggactc ccgatttact gacggtgaca cgtctgagga tcgcaagacc
     5941 aggagagggc agagctatgt gtccgccgct ggtgaccgtg gcgggcttgt ggaatcgccc
     6001 gagcgagctc ctcccgaagg cgggcggaga cactgggaac acgcgaaacg ccggtctttt
     6061 gggaagttgc agagagggct aacgggccaa catactgcgg ggaagatatt gctgggacaa
     6121 tggtccgtct ccccgagagg gacgcagctc ccgaatgatt catggagaat gatactctat
     6181 gaagccgctt gcgtctctct tcctgcttct tgccgccgga acccccggct cgcaagccgt
     6241 tcaggactac ttccccatgg ccgtctggta cgggggcggc aaagcccgtg ctcccatgct
     6301 cgagcgcgac ccgcacgcca agaaggagac ttggcggaaa gatctccagc agatcaagag
     6361 cctcggtttc aacaccatcc gctgctggat cgactgggcg agcggcgagc cctccgaagg
     6421 cgactaccgg ttcgacaccg tcgacgtgct cctcgaactt gccgagcagg agggcctgaa
     6481 actcgttatc caggtctaca tggacgccgc ccccgactgg gtcggcaaga gatacccgga
     6541 ctcgctgttc gtcgcagcgg gtggcgaagt gatgcgtcct gagcccgcgc ccggctactg
     6601 tttcgaccac gccggcgtgc agaaagcgac actcggcttt ttcaccgctc tggcccgccg
     6661 tgcagcaaaa agcccggcct tcctcggctg ggatctgtgg agtgagccgc acgtcatcaa
     6721 ctgggcaatg gctccgtact taccgaacgc cgagttctgc ttttgcccgc attccgtcgg
     6781 gcgttttcga agctggctca agaaaaaata cggtacgctc gatgcgctga acgtcgcttg
     6841 gtaccgccga ttcagcgact ggaatcaggt ggaaccgggg cgcctcagca cgatcctctc
     6901 ctacaccgac tatatcgact ggcgcacttt catccaggag aagctcggtg aagacctccg
     6961 gatgcgctac gaggccgtga agcgcggggc gccggaccgg gtcgccacca gccacgcggc
     7021 cgtgccgagc ctgttcacct cgccgctcgc aggcgacggc gcgcccgatg actggatcat
     7081 ggcccgccag gcagactact atggcacttc gttctacccg aagcactcaa gccctgtcgg
     7141 acgcgatgtc ccgtggcgcg cggcgctcat ggacttcgcc cgctcctccg ccaaagatcg
     7201 cggcttctgg gtcggcgagc tacagggcgg ttttggaacg atcgctctgc gcgtgagcgc
     7261 tacggtcact cccgatgacc tccgcgtctg gacctggacc gccctctccc gaggcgccaa
     7321 aggcctcaac ttctacgcct ggtacccgat gagttccggt tacgagtcgg gcggatacgg
     7381 tttgattcaa ctcgacggca cgatcaccga gcgttcgcgc gccgccggag aaattgcgcg
     7441 cgtggtggat cgcaaccaaa agctgttcct cgacgcccgg ccgccaaagg ccgaggtcgc
     7501 catcgtctat aacccgatgg tgcacttcgt cggcggccgg cagcgggcct tctcgtcgcc
     7561 cgccgcgcag ggcgaggtgt tcggcatcga gcgtgactcg ctgctcggca cataccgcgc
     7621 gctcttcccc acaaatgtcc ctgtggattt cgtccacatc aacgagatca cggtggcaga
     7681 gctgaagcca tacaagctcg tcatttttcc gtacccgttg atgatgccag gccgtgtcgc
     7741 gacggaactc gccggctatg tgcgcgccgg cggtgcgctg gtttccgagg cgcgcctcgg
     7801 ctggaataac gaacaagggt gggccgcgga aaccatcccc ggtctcggcc tccacgagat
     7861 catgggctgc cgcgaaactg cgatccagac gaccccgaac aaccgtacgg aactgacctg
     7921 gaccggcgac ggcatcccgg gactgaaacc gggcgaccgc ctgccgggcc gcttgtacga
     7981 agagacgctc gagcccctgg gagcgcaatc gaaagccgtt gcgaaacacg ccaacggcgc
     8041 tcccgcagcc gtcatctcgt cgttcggcgc gggtaagacg ctgaccctcg gcacttacgt
     8101 cggagttgcc tacgaggcgc aacaggatcc ggcggttcag cgtttgttca caggccttct
     8161 cgaatgggcc ggcgtcacgc gtccggtgga atcctccgcc gcagacactg aagtgcgctg
     8221 gctcgagtcc ggaagcgacc gcatcgtttt catcttccac cacgcggaca aaccgtccga
     8281 cgtcccggtt gcgctgtcga tgccgccggg caactggact gcggtcgacc ttcagagcgg
     8341 ccgagtagtg gagttgaacc gatccggagc gtctgttcgt tttggcaaga gactaggtcc
     8401 atccgaggtc tgggtgctaa gacttagccc ggagtagccc cattgactcg cagcgcggtt
     8461 gggagtactt acgaagcagg ggcccggctc gatgtcgctg ggcgccggtc cccatggagg
     8521 tgagcgatgc tctcccgcac cctcgttcaa atgatcgagg atcactgcga gcagattaca
     8581 gaccggctcc tccggcacca gcggcaggat cccgagctca ttcaggtaaa gaggctgccc
     8641 gagtccgagc tgcgcgatcg ggcgggcgaa gtcctgaaga acctcggtca ctggttgtcc
     8701 gccgggcgtg aagccgagtt gtcaagccgc tacgaactgc tcggtcaccg ccgtttcgag
     8761 gaggccatcc cactatacga ggtggtgcgc tcgttgcacc tactcaaaac aaacatgatc
     8821 gagtacgtcc gtgaacaggg gatcggccaa aaccccgtcg agttgtacgc ggaagaggaa
     8881 ctcgagcacc gggtcgggct cttcttcgac ggcgccgtgt atcacacggt acacggctac
     8941 gagaccgcgt tacggaagaa ggcgcacctc gccgcgtgag cgggtctcca gcgccggtgc
     9001 gctaccaagc gaaaggggag cggatcgact cctgatgaca gtcggtcccc aggcgcgtcg
     9061 ccgcaacatc gaggatctgc gaacgggatc gctggatgtc ctcgtgatcg gcggcggcat
     9121 taatggagtg ggcgtggccc gcgacctggc cttgcgctcg gatcacgcag gcgccgggct
     9181 gcgcatcggg ctggtggaac agcaccactt cgcctccggc acaagcggca agaactcaca
     9241 gctcattcat ggcggactgc gctacctgaa gtactttcag ttcggcatgg tccgagaaag
     9301 cctccgtgaa cgggcactgc ttttgagact cgctccacat ctcgtcgaac ctctgccgct
     9361 tctgattccg atgttcagct cgttcgatcg cttctactat ggcgccgggc tgtgtctgta
     9421 tgacctcctc gccgggtcca gaaacatcgg aaaacaccgc cgcctgtccc tgccggccgt
     9481 ggccggtctc gaaccgggtc tgtcccacga acgtctgtcc ggaggcgcga tcttcttcga
     9541 ctgccgggta cattcggcac gtctcgtcct ggaaaatgtc ctcgacgcag cccgcctcgg
     9601 aacgatcatc gcgaactatg tgctggctgt ggacctccgc cgcgagagcg gccgctggcg
     9661 agtttcgatc gaggacgcct tctccggcga gcggttcgag gccactgccc gggaaatcgt
     9721 cgacgcggcc ggaccgtgga gtacaggcgc gccgctccgg ctggttcgag gctcccacct
     9781 gattctgccg cggatcacca ctggcgagcg cgccatcgcg catttcgacg agaacgggcg
     9841 gatcgtgttc ctgatcccct ggggcagtca caaacaactc acgctcgtcg gaacgactga
     9901 tgtggatcac gagtccgggc ccgacgacgt cgagatctcg cccgaggaga gggcctatct
     9961 gctccggatc gtgcgtaaac ttttccccgg agccgctggc gtggagccgg tctcaacctt
    10021 cagttccctg cggccgctac tgcgcgatgg ctccggctca ccggtggccg ccagccggga
    10081 gtaccggatc tggaggtcgg acgacggcat cgtccgcatc gccggcggaa agtacaccac
    10141 ctaccgttcc atgagcgaac aggcggccga tctcgttctg cggaacatcg cgccggaact
    10201 ggcccgcatg cacgtcaccg cggaggcacc gttcccgccg ccggaagggc gccgcatcga
    10261 ggaccttccc gtcgacgaac aggcatcgtt agttgtgaat tgcgagatgg cgcagcgttt
    10321 gcccgatctg ctgttcgttt cgacctacct gggttacgag cggatttggg atcgagccgc
    10381 cttgcgtccc ttcgccgaag cgatggcgct tcacctcggt tgggacgaac ggcgtatgca
    10441 cgaagagatc gacctcgtgc tgcggctgtg cggccgccct ccggcgagta cactgaggac
    10501 gtgagcccgt actggcgcac tttctggtgg ctgttccggc gttccttcgt cctcgccttc
    10561 caggacagtt gtttcggcgt cgccaaggca gcggcttact cagctttgct gtcgctgttt
    10621 ccactactta cggcgaccgc caccattctc gtgcaggtgc gggcagaacc gatcacgcgt
    10681 gtgatctcac gggcgctctc gcaagtgctc ccgcccggta ccgaggacct cgtcctgaac
    10741 cacttccgcg tgcacggtga acggccgtcc ggcttgctcg tcgccgcgac cctgatctcc
    10801 atctgggcag cgtcgcgcgt aatgacgagc ctgatggaag ggttccaggc tgcataccac
    10861 gtccccgtag accggccctt cctgcgccag cagggcatgg caatcttgct cgtcttcgcc
    10921 accgccgttc cggttgttgc cgcgtccggc ctcatcctgt ttggcacgcg gatcgagcgg
    10981 gcggtggtgg cgtggctagg gctggtccca gccggggccg atttgcggtc gggagtcgcc
    11041 ttcacgagca gcgtcctgcg attcgccgtg gcgttgggct cgatgatcct cgtgaatgcc
    11101 atgatgtacc ggctcggccc gaatcgcccc cagcgatggc gtaacgtgtg gcctggggcc
    11161 atcgtcgcca ccgt
//
LOCUS       DATIFQ010000110         4306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2334417, whole genome shotgun sequence.
ACCESSION   DATIFQ010000110 DATIFQ010000000
VERSION     DATIFQ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2334417"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VLE22_04665"
     CDS             complement(<1..367)
                     /locus_tag="VLE22_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13727.1"
                     /translation="MTSSFDYEPLGRPEKVTEAGMRETNTVYDDENLAVLMKADLDTK
                     ADGKLTTVRHFDPLWRVRLERTTDGTPLALGSEEAGIKVQRRYRTVTGARYELASNPY
                     RAATSGAASSEQTMGWTRTK"
     gene            884..3184
                     /locus_tag="VLE22_04670"
     CDS             884..3184
                     /locus_tag="VLE22_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HSB13728.1"
                     /translation="MRGPRNFSAASIVYHQLMSRPSFFLALLCVCTSVLPAAGGRGRI
                     NYFLGNDPSRWEVDNRSSPSPRQAAGSGPYSVYIGGSKEVQPARIVTDAQGNVFVAGS
                     TVWNAVAWWGGEITFSDVFVSKIDANGEPVFTTYFGGDGFDTAYGLTIDATGRVYVVG
                     MTSSVNFPTVNAYQKQPSRLPDAFVCVLDANGGFVYSTYLGGLRDETAFAVAADAAGN
                     AYVTGTTSSGDFPWTPGAYQSAPVPGGNALVSPSGVFVSKFSPDGRTLVYSASLRGTR
                     VVCVGGSRCVFAVSRDNGTAIAVDADGNAYVGGSTNSVDFPVTPGVLQPVCKCDYFST
                     DAFVSKLNPSGSALVYSTHLGGSVLVDVMRGESVAGLRVDASGRVYVTGSTSSPNFPT
                     TAGAYRAAPAGEPTDALYGFVSELAPDASRLVYSTLLNGKGQVETRGFSLDPQGSAWV
                     AGTTTNSDLPVSQGGFPRGEDFLARLNADGSALAYSTLLPNAFGGKDVALDLAGNAYL
                     LGNAGYVSRIEGDSYRLPAVLGIANAAGDQVTGRISSGELISIFGNGIGPDSPVGLEL
                     TPEGKVATKLAGTEVWIGGIPAPLTYAQKDQINAVTPYYAGQTPQVRIVRDGATVGEI
                     RLSAIPADPGVFRYPPDPATPGALRRAAALNEDGSVNSSANPAKPGSVVSIFATGLGA
                     TEPPLEDGAVSLEKLPKPKLPISVEIVGPQPEVEVTYAGQAPGLVAGAMQVNFRLPEG
                     TVSGRLFLALKAGEFRSGMFAVEVGP"
     gene            complement(3187..>4306)
                     /locus_tag="VLE22_04675"
     CDS             complement(3187..>4306)
                     /locus_tag="VLE22_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13729.1"
                     /translation="DAEQTRDFVLSYTRGDRYKPLPGYKTMVTHFHTAFAEELMQAKS
                     LDVQAPWIPAIRALGADIAHIHDFHGDGHPQDTGPLRLKELENYFAACRRHSDRDFLI
                     LPGEEPNAHFGGHYNILFPKPVYWTLRRAPGQQFLEQHPTYGTVYHTGSSEEVLEMAK
                     RENALIWTTHPRTKNSTGYPDKMRDSEWFRSDRWLGGAWKSLPVDLSEQRLCERRCFD
                     VLDDMNNWGAPKYILGEVDTYKKFPEYDLYGDFNVNYLRLEALPSGDDWTPIVRALRA
                     GDYFVTTGEVLIRDFRVEGNSAVTAEVEWTFPLEFVEVVWGDGRTVGRKIIPATDLPA
                     FGSRRFRVPVDLTGKKWVRFAAWDSAVNGAFTQPAHLK"
BASE COUNT          776 a         1292 c         1378 g          860 t
ORIGIN      
        1 gcttggtccg cgtccaaccc atcgtctgtt cgctcgatgc ggcgcccgac gtcgccgccc
       61 ggtaggggtt ggacgccagt tcgtaccgcg ccccagtcac cgtgcggtac cgccgctgca
      121 ccttgatacc cgcctcctcg ctccccagag ccaatggcgt cccatcggtg gtccgctcca
      181 gccgcaccct ccacagcgga tcgaagtgcc gcaccgtggt cagcttcccg tccgccttcg
      241 tgtccaggtc ggccttcatc agtaccgcca ggttctcgtc gtcgtagacg gtgttggtct
      301 cccgcatccc cgcctcggtc accttctccg gccgccccag cggctcgtag tcgaagctgc
      361 tagtcacgcc gttgttggtg acataaccta tctgattgat atctaataac ttacgtaatg
      421 attcccgaag ccaaggctgt attatgagat cggggaacca aatgtcgtca atcagaagaa
      481 ttctttcatc acgggtcaac ggagcccgtt ccagagggcc ggtcacgctg gggggcaata
      541 gcgaccagtt ggaccgcatg acggcggctt ttgcgaatct agctgccaaa ccctcagccg
      601 ccctgctgca ccgttacgaa acgcgcctcc atctcatgta tcaccgcgcg ttacaaaatc
      661 tgcttctgtt gcgcctcgcc ataccaaacg aacccagtcc catttccgaa cacctggggt
      721 aggcttcccc ctggtcgggg gacgtacact ccgctgcgcg ggaaacccgt gaatgagtcc
      781 ccggttttga aattagattt caacaatatc gagaggtgat agtacgaaaa ttaaccggaa
      841 tggtactgga aattcaacgg aatcgcgcgg ggctggcgag gaaatgcgcg gcccgcgcaa
      901 cttttcggct gcttccatcg tctatcatca gttaatgtcc aggccttctt ttttcctggc
      961 actgctctgc gtctgcactt cagtgctccc cgcggccggc ggccgcggga gaatcaatta
     1021 cttcttaggc aacgatccca gtcgatggga ggtcgacaat cggagcagtc cgtcgccgcg
     1081 ccaggccgcc ggctccgggc cgtattcggt ttacattggc gggagcaagg aagtccaacc
     1141 ggcacgaatc gtgaccgacg cgcaggggaa tgtattcgtc gcgggcagca cggtgtggaa
     1201 cgccgtcgcc tggtggggcg gggaaatcac gttctccgac gttttcgtct ccaaaattga
     1261 tgcaaacggc gagccggtct tcacgacgta ctttggcggc gacggcttcg atacagcgta
     1321 cggcctgacg atcgacgcca cgggcagagt gtacgtagtg ggaatgactt cgtcggtgaa
     1381 tttcccgacg gtcaacgcct accagaagca accgtcgcgg ctaccggacg ctttcgtttg
     1441 cgtgctcgac gcgaacgggg ggttcgtcta ctcgacctat ctcggcgggc taagggatga
     1501 gactgcgttc gccgtggcgg cggacgcggc ggggaacgcc tatgtgaccg gaaccacctc
     1561 atccggcgat ttcccgtgga caccgggcgc ctaccagtcg gctccagtcc cgggcggcaa
     1621 tgcgctcgtg agcccgtccg gcgtctttgt ctcgaaattc agcccggacg gcaggacgct
     1681 ggtctactcg gcgtcactgc gagggacgag ggtggtatgc gtgggcggca gccggtgcgt
     1741 ttttgcggtt tcgagagaca atggcaccgc gatcgcggtg gacgcagatg gaaatgcgta
     1801 tgtcggcggg agcacgaatt cggtcgactt cccggtgacg cctggcgtgt tgcaaccggt
     1861 ctgcaaatgc gactattttt cgaccgatgc gttcgtcagc aagctaaacc cgtccggaag
     1921 cgcactggtc tactcgaccc acctcggagg gtccgtgctg gtggacgtga tgcgcgggga
     1981 atccgtggcg ggattgcggg ttgatgcctc gggacgcgtg tatgtgaccg gctcgacatc
     2041 gtctccgaac tttcctacga cggctggagc gtaccgcgcc gctccggcgg gcgagccaac
     2101 cgacgccctg tatggcttcg taagcgaact cgctccggat gccagccggc ttgtgtactc
     2161 caccctgctg aacggaaagg gacaagtcga aacgcggggc ttctcgctag acccgcaagg
     2221 aagcgcctgg gttgccggta cgaccacgaa ctcggacctg ccggtttcgc agggcggttt
     2281 cccacggggc gaagacttcc tcgcacggct caacgctgac ggatcggcgc tggcgtattc
     2341 cacactcctg ccgaatgctt tcggaggaaa agatgtcgca ctcgacctcg cgggcaatgc
     2401 ctatctgctc ggaaacgccg gctatgtctc gcggattgag ggagactcgt accgtcttcc
     2461 ggcagttctc gggatcgcga acgcggcggg tgatcaggtg accggaagga tctcctcagg
     2521 agaactgatc agcattttcg gaaacggaat cggcccggac agcccggtgg gactggaact
     2581 cactccggag ggcaaggttg cgacgaagct tgccgggacc gaggtgtgga tcggaggtat
     2641 cccggcgccg ctgacgtatg cgcagaaaga ccagatcaac gcggtgacac cgtactacgc
     2701 cgggcagacc ccacaggtac ggattgtgcg tgatggggcg actgtcggtg agatccggct
     2761 gtcggcaatt ccggccgatc caggcgtgtt ccgctatccg ccggacccgg caaccccggg
     2821 cgctttgcgc cgtgcggccg cgctgaacga agacggatcg gtcaactcgt cggcgaaccc
     2881 ggccaaacca ggatcagtgg tttcgatctt tgcgaccggc ctgggagcga ccgaaccgcc
     2941 gcttgaagac ggagcggtgt cgcttgagaa gctcccgaaa ccgaaattac ccatttcggt
     3001 cgagatcgtt ggtccgcagc cggaggtcga agtcacttac gccggccagg cgccaggtct
     3061 ggtggcggga gcgatgcagg tcaacttccg cttgccggaa ggcactgttt ccggccggct
     3121 ctttctggcc ctcaaagccg gcgagttcag gagcggtatg ttcgccgtcg aggttgggcc
     3181 gtaacttcac tttaggtgtg ccggttgggt gaatgcaccg ttgacggctg agtcccatgc
     3241 tgcgaaacgg acccatttct tgccggtgag gtccaccggg actcggaacc ggcgcgaccc
     3301 gaacgcaggc agatccgtgg cggggatgat cttgcggcct acagtgcggc cgtcgcccca
     3361 gacaacttca acaaactcaa ggggaaacgt ccactccacc tccgccgtta cggctgagtt
     3421 cccttcgacg cgaaagtctc ggatcagcac ttcgcctgtg gtgacgaagt agtcgccggc
     3481 acgcagcgcg cggacgatcg gcgtccaatc gtcgccggag ggtagtgctt ccagacgaag
     3541 atagttcacg ttgaagtcgc cgtaaaggtc gtattcggga aacttcttgt acgtgtccac
     3601 ctcgccgagg atgtacttcg gcgcgcccca gttgttcatg tcgtcaagga cgtcgaagca
     3661 gcgcctctcg cacagacgtt gctcggaaag atcgaccggc agggacttcc atgcgccgcc
     3721 gagccagcgg tcgctgcgga accattccga atcacgcatc ttgtcgggat agccggttga
     3781 gttcttcgtg cgcgggtgag tcgtccagat caacgcgttc tcccgtttcg ccatttcgag
     3841 gacttcttcc gagctgccgg tgtggtagac ggttccgtat gtcgggtgtt gttcgaggaa
     3901 ttgctgtccc ggcgcgcggc ggagagtcca gtagaccggt ttcgggaaga ggatgttgta
     3961 gtggccgccg aagtgcgcgt tgggctcctc gccggggagg atgaggaaat cgcggtccga
     4021 gtggcggcgg caggcggcga aatagttttc gagctccttc aggcgcaagg gcccagtgtc
     4081 ctgcgggtgc ccgtcgccgt ggaagtcgtg gatgtgcgcg atgtcggcgc cgagagcccg
     4141 gatggccgga atccatggcg cctgcacgtc caggctcttg gcttgcatga gttcttcggc
     4201 gaaggcggtg tggaagtgcg tgaccatcgt cttgtagccg ggcaggggct tgtagcggtc
     4261 gccgcgggtg taggagagca cgaaatcccg cgtctgctcc gcatcg
//
LOCUS       DATIFQ010000111         8804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1759821, whole genome shotgun sequence.
ACCESSION   DATIFQ010000111 DATIFQ010000000
VERSION     DATIFQ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759821"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VLE22_04680"
     CDS             complement(<1..345)
                     /locus_tag="VLE22_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13730.1"
                     /translation="MSNFTQQLQKMKTHPGFVAALDQSGGSTPSALRSYGIKEEAWSN
                     EDEMFAIVHQMRTRIITSPSFTGDRILGAILFENTMDREIAGSPSADYLWNVKQVVPF
                     LKVDQGLAAETGG"
     gene            complement(403..2058)
                     /gene="pgi"
                     /locus_tag="VLE22_04685"
     CDS             complement(403..2058)
                     /gene="pgi"
                     /locus_tag="VLE22_04685"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HSB13731.1"
                     /translation="MKATAKPLTQRPAWKALTVHHQEIRELHLRHVFAKDPKRGERLT
                     AEAVGLYLDYSKHRITDETLRLLLQLAEESDLRGRIDAMFRGEKINITEGRAVLHVAL
                     RAPREASIAVDGENVVPQVHAVLDKMEAFSTRLRRGAWTGYTGKPIRNVINIGIGGSD
                     LGPVMAYEALKHYSDRTRTFRFVSNIDGTDFLESVRDLDPAETLFIVSSKTFTTLETM
                     TNARSARAWSLAGLGGNEKSVAKHFVAVSTNAAEVAKFGIDTANMFEFWDWVGGRYSM
                     DSAIGLSTMLAVGPEHFRTLLAGFHQMDEHFRTAPFARNLPVLMGLLGIWYSNFFGAQ
                     TVAVLPYEQYLKRFPAYLQQLTMESNGKHVTLDGTEVSYPTGPVYWGEPGTNGQHSFY
                     QLIHQGTPLIPCDFIAFSQSLNPLGRHHDLLLANVFAQTEALAFGKTAEQVKAEGTPD
                     RLVPHRVFEGNRPSTTILAERLTPETLGKLVALYEHVVFTQGTIWQIDSFDQWGVELG
                     KVLAQRIAPELESQGEPALGHDSSTNALIRRYRQLKAHEGHRV"
     gene            complement(2055..2804)
                     /locus_tag="VLE22_04690"
     CDS             complement(2055..2804)
                     /locus_tag="VLE22_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSB13732.1"
                     /translation="MRRVLVIDIGGTHVKVLASGQPNEREFVSGPKLTPKRMMSEVQS
                     LVADWKYDVVSIGYPGPVRRGRPVAEPWNLGKGWVDFDFEDAFKRPVKVINDAAMQAL
                     GSYRHANMLFLGLGTGLGSALVVDGIVVPMELGHLPYKKATFEDYVGVRGVVKYGKKK
                     WRRHVTDVVERLLAAIEPEDVVLGGGNVKNLKKLPLGCRAGNNANAFLGGFRLWKENG
                     APQNLSRARQRTHEREADRSNRPKGTKGTRR"
     gene            complement(2925..5297)
                     /locus_tag="VLE22_04695"
     CDS             complement(2925..5297)
                     /locus_tag="VLE22_04695"
                     /inference="COORDINATES: protein motif:HMM:NF003616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HSB13733.1"
                     /translation="MNRRRTKAAPLSPDLLRKMDAYWRAANYLSVGQIYLYDNPLLKR
                     PLRREHIKPRLLGHWGTTPGLNLIYVHLNRVIKERDLNVMYITGPGHGGPGLVANTYL
                     EGTYSEVYPDVSQDEAGLKKLFKQFSFPGGIPSHVAPETPGSIHEGGELGYALSHAYG
                     AAFDNPDLIVACVVGDGEAETGPLATSWHSNKFLNPVRDGAVLPILHLNGYKIAGPTV
                     LARLPQEELESLFRGYGYVPHCVEGDDPKDVHQQMAAAFDTVFTDIRRIQEEVRRKGF
                     RQRPRWPMIVLRTPKGWTGPTNVDGKIIEGTFRAHQVPMGDMEKPAHIKILERWMKSY
                     KPHELFDKTGRLRPELADLAPKGRHRMGANPHTNGGLLLHDLKLPDFRNYAIKVSRPG
                     SVVAEATRVQGAFIRDVMKLNPENFRVFSPDEAASNRWTAVFEVTNRCSTAQILPTDE
                     HVSPDGRVMEMLSEHQCEGWLEGYLLTGRHGFFSCYEAFIHIVDSMFNQHAKWLKVTR
                     HLSWRRPIASLNYLLSSLVWRQDHNGFSHQDPGFIDHVVNKKAEVIRVYLPPDANTLL
                     SVTDHCLRSRNYVNVIVSGKQPELQWLDMDAAIKHCTAGLGMWEWASNDRGGDPDVVM
                     ACAGDVPTLETLAAVQVLREHFPDVKVRVINVVDLMTLQPQHEHPHGLDDKDFDALFT
                     KDKPIIFAFHGYPWLIHRLTYRRTNHTNLHVRGYKEEGTTTTPFDMVVLNDLDRFHLV
                     GDVVDRVPSLEARAAYVKQFIRDKLLEHKEYVQTHGQDLPEVRDWKWKAT"
     gene            complement(5257..6546)
                     /locus_tag="VLE22_04700"
     CDS             complement(5257..6546)
                     /locus_tag="VLE22_04700"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HSB13734.1"
                     /translation="MPVRRERSGDTGRAQSGSTGVLTVNGGSSSIKFALFEADGRLRR
                     ILQGRIEGIGLPQGSFAAKGLNPGDTFSRRVVAPEHGTAVSLLMDWVQERIERSAVTA
                     VGHRVVHGGPRYWEPQPITPEMLETLQQLSAFDPEHLPEEILLAQTFHRRFPSLPQIA
                     CFDTAFHHEMPRVARLLPIPRRYDARGVRRYGFHGLSCAYLIEELARLAGAGTANGRV
                     VLAHLGNGASITAVREGKSIDTSMGFTPTAGMPMSTRTGDLDPGLGWYLARTEGLTAK
                     QFHRMVNHESGLLGVSESSSDMRELLGRESTDIRAAEAVALFCYQAKKWIAAMAAALG
                     GLDTLIFAGGIGENAPEVRARICRGLEFLGFTLDGKRNGTSAPVISADGSAATVRVIR
                     TDEEWIIAKTVCRIVGLSLERDTDHEQEKNQGRAAVP"
     gene            complement(6547..7632)
                     /gene="tal"
                     /locus_tag="VLE22_04705"
     CDS             complement(6547..7632)
                     /gene="tal"
                     /locus_tag="VLE22_04705"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HSB13735.1"
                     /translation="MKATQLLHDLGQSLWLDNITRDLLTSGTLRRYIDELSVTGLTSN
                     PTIFDQAIKNSTAYDAVIHGKLAKGTSGEDLFFEVALEDLTQAAHLFRPVHERTNRVD
                     GWVSLEVSPLLAYNTEKTLAAAKSLHARAGEPNVLIKIPGTREGLPAIEGAIFAGVPV
                     NVTLLFSREHYLAAAEAFLRGIERRIALGLNPGVGSVASLFVSRWDAAVAGKVPATLR
                     NQLGIAMAKRTYKAYRTLLESPRWQRAYNAGARPQRLPWASTGTKDPQASDVLYIKAL
                     AAPFTVNTMPEGTLTALADHGEIGAPLPPDGGDCEDMLAQFVKAGIDIDALAAQLQDG
                     GAKSFVKSWNDLLAVIGSKSAALTAKV"
     gene            complement(7719..8315)
                     /locus_tag="VLE22_04710"
     CDS             complement(7719..8315)
                     /locus_tag="VLE22_04710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSB13736.1"
                     /translation="MSESLPVIYLARHGETAWSLSGQHTGLTDLPLTERGERNARSLG
                     KRLQGRTFATVLTSPLQRAARTCDLAGFGAEAAMDRDLVEWNYGEYEGRRTIEIRAER
                     PDWQLFRDGCPGGETPEQVGTRADRVVSRVREARGDALLFSSGHFLRVLAARWVGLPP
                     GAGRYFLLGTASLSALGYEDGLPRPVIRFWNDTSHVEL"
     gene            complement(8312..>8804)
                     /locus_tag="VLE22_04715"
     CDS             complement(8312..>8804)
                     /locus_tag="VLE22_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSB13737.1"
                     /translation="FRRLSEGVFDLDDLRARTHELADFVEAAARRYRLASDRILAVGF
                     SNGANIAASVLLLRPRVLAGAVLFRAMVPLVPETLPDLRGTPVFLGAGRRDSIVRPDE
                     PERLRRLLVQAGASVTLHWEQGGHGLIPADIEAAHAWLEGWAAARGDRAPAKANSPKG
                     TPA"
BASE COUNT         1558 a         2814 c         2722 g         1710 t
ORIGIN      
        1 cccgcccgtc tccgcggcca ggccctgatc cactttcaga aacggcacca cctgtttcac
       61 attccacaga tagtccgcgc tcggcgaccc ggcgatctcc cggtccatgg tgttctcgaa
      121 caggatggcg cccagaatcc ggtcgccggt gaagctgggg ctggtgatga tgcgggtgcg
      181 catctgatgc acgattgcaa acatctcatc ctcgttggac cacgcctctt ccttgattcc
      241 gtaggaacgc agcgcactgg gcgtgctgcc accgctttga tccagcgccg caacgaaacc
      301 gggatgggtc ttcatcttct gcagttgctg tgtgaagttg ctcatcttaa tcacctcaga
      361 tcttggagat aaggtttgcg agtactctag ccacaggcct ggtcacaccc gatggccctc
      421 atgtgccttg agctgccgat agcgacggat cagggcgttc gttgaactat catggccgag
      481 cgcgggttcc ccctggctct ccagctccgg ggcaatgcgt tgggccagca ccttgcccag
      541 ctcgacgccc cattggtcga aggagtcgat ctgccagatc gttccctggg tgaagaccac
      601 atgttcgtag agtgccacga gcttcccgag ggtctccggc gtgagtcgct cggccaggat
      661 cgtggtcgac ggtcgattgc cctcgaagac ccggtggggg acaagccggt cgggcgtgcc
      721 ctcggccttg acctgttcgg cagtcttgcc aaaggccagc gcctcggtct gggcgaagac
      781 attcgccagg agcaggtcgt gatgccggcc caggggattc agcgattggg agaaggcgat
      841 gaagtcgcac gggatgagcg gggtcccctg gtggatcaac tggtagaagg agtgctggcc
      901 gttggtcccg ggctcgcccc agtagacggg gcccgttggg tagcttacct cggtgccgtc
      961 gagggtgacg tgcttgccgt tgctctccat ggtcaattgc tggaggtagg cggggaagcg
     1021 cttcaggtac tgctcgtacg gcagcaccgc tacagtctgg gcgccgaaga agttgctgta
     1081 ccagatcccc aggaggccca tgagcaccgg aaggttccgg gcgaagggcg cggtgcggaa
     1141 gtgctcgtcc atctgatgga agccggcgag gagggtgcgg aagtgctccg gtccgacggc
     1201 cagcatcgtg gagagcccga tggccgagtc catggagtag cgccccccga cccagtccca
     1261 gaattcgaac atgttggcgg tatcaatacc gaacttcgcc acttccgcgg cgttggttga
     1321 cacagcgaca aaatgcttgg ccaccgattt ttcgttaccc cccagacccg ccagcgacca
     1381 ggcgcgcgcg ctccgggcgt tggtcatcgt ttccagggtc gtgaaggtct tcgaagagac
     1441 gatgaaaagg gtctcggccg ggtccaggtc gcgaaccgac tctaggaagt cagtgccatc
     1501 gatgttggac acgaaccgga acgtccgggt gcggtcgctg tagtgcttga gggcctcgta
     1561 ggccatgacc ggaccaaggt ccgaaccccc gatgccgatg ttgatgacgt ttcgaatggg
     1621 cttgccggtg tagcccgtcc acgcaccgcg ccggagtcgg gtggagaagg cttccatctt
     1681 gtcgagcacg gcgtgaacct gggggaccac attctcgccg tccaccgcga tggaggcctc
     1741 gcgcggagcg cgcagcgcca cgtgcaacac ggcccgccct tccgtgatgt tgatcttctc
     1801 gccccgaaac atggcgtcga tccgcccccg caggtcggat tcctcggcca attgcaggag
     1861 aagcctgagg gtttcgtcgg tgatccgatg tttggagtag tccaggtaga ggccgaccgc
     1921 ctcggccgtc aggcgctcgc cgcgtttcgg atccttcgcg aacacgtgcc ggagatgtaa
     1981 ttcccggatt tcctggtgat ggacggtaag cgccttccat gccggcctct gggtgagcgg
     2041 tttggcggtt gccttcatct tcgcgtccct ttcgtgccct tcgggcggtt ggatcggtct
     2101 gcttcacgct catgggtgcg ctgtctggca cgagacaagt tttgtggggc accgttctcc
     2161 ttccatagac ggaaccctcc aagaaacgcg ttggcgttgt ttccggcacg acaccctaag
     2221 ggaagctttt tcagattctt gacgttccct ccgccaagga ccacgtcctc aggctcgata
     2281 gcagccaaca ggcgttctac gacatcggtc acatggcgac gccacttctt cttgccgtac
     2341 ttcaccacgc cccggacacc gacgtagtcc tcgaaggtgg ccttcttgta cggcaagtgc
     2401 cccaactcca tcggcacaac gatgccatcc acgactagag cggatcccaa gccggtgccc
     2461 aagcccagaa agagcatgtt ggcgtgcctg tagcttccca aggcttgcat ggcggcatca
     2521 ttgatgacct tgacggggcg tttgaaagca tcctcgaagt cgaaatcgac ccaccctttt
     2581 cccagattcc agggttccgc cacgggccgt ccgcggcgga ccggccccgg gtacccgatg
     2641 gagacgacgt catacttcca atcagcgacg agactctgaa cctcggacat catgcgcttt
     2701 ggcgtcagct tcggccccga cacgaactcg cgttcgttcg gctggccact agcgagaact
     2761 ttgacgtgcg ttccaccgat gtcaattacc agcactcttc gcatgtacgt cgagttcctt
     2821 tccgtattcc gatggcgttc ctttatgagc caatggaacg gtcagcagat attcacccgc
     2881 caagaagcca gctctaccta gtccggactc catacatagg tggtctatgt ggctttccac
     2941 ttccagtctc tgacttctgg caagtcctgg ccgtgcgtct gaacgtactc cttgtgctcg
     3001 agcagtttgt cgcggataaa ctgcttcacg tatgcggcac gcgcttccaa gctgggcacg
     3061 cgatcaacca catctcccac aagatggaaa cgatccaggt cgttgagcac gaccatgtca
     3121 aacggcgtgg tggtcgtccc ctcttccttg tagccgcgaa cgtgcagatt cgtgtgattg
     3181 gtacgccgat acgtcagccg gtggatcaac caaggatagc cgtgaaaggc aaagatgatc
     3241 ggtttatctt tcgtgaacag cgcgtcgaag tctttgtcgt ccaagccgtg cggatgttcg
     3301 tgctgcggct gaagcgtcat caggtctacg acgttgatga cccggacttt cacatccgga
     3361 aagtgctcgc gaagaacctg aaccgcagcc aatgtctcca gcgtcggcac gtcgcccgcg
     3421 caggccatca ccacatccgg atcgccaccc cggtcgttgc tggcccactc ccacatgccg
     3481 agtccggccg tgcagtgctt gatggcggca tccatatcca gccactgcag ctccggctgt
     3541 ttgccgctga cgatcacgtt cacgtaattt cggctgcgca ggcaatggtc ggtgaccgag
     3601 agcagcgtat tcgcatccgg gggaagatag actcggatca cctcggcttt cttgttcacc
     3661 acgtgatcga tgaagcccgg gtcctggtga ctgaacccgt tatggtcctg ccgccaaacc
     3721 agcgaactga gcaggtaatt caacgacgcg atgggacgtc gccatgagag gtgccgcgtc
     3781 accttcagcc acttggcgtg ctggttgaac atcgagtcca cgatgtgaat gaaggcctcg
     3841 tagcaggaaa agaacccgtg ccgacccgtg agcagatacc cttccagcca gccttcgcat
     3901 tgatgctcac tcaacatttc catcacgcgg ccatccggcg acacgtgctc gtccgtggga
     3961 aggatctgcg cggtcgagca gcgattcgtg acctcgaaaa cggcagtcca gcgattcgag
     4021 gcggcttcat cgggactgaa gactcggaaa ttctccggat tgagcttcat cacgtcgcga
     4081 atgaatgcgc cctgcacgcg cgtggcttct gccacaacgg agccgggccg cgagaccttg
     4141 attgcgtagt tgcggaagtc cggcagcttc aggtcgtgga gcagcagccc gccattggtg
     4201 tgcggattgg cacccatgcg gtgtcggcct ttgggtgcaa gatccgccag ttccgggcgc
     4261 aatcggccgg tcttatcaaa gagttcgtgc ggcttgtagc tcttcatcca tcgttcgagg
     4321 attttgatgt gcgccggctt ctccatgtcg cccatgggca cttggtgcgc gcgaaatgtg
     4381 ccctcgatga tctttccgtc gacattcgtt ggtccagtcc agcctttggg ggtgcggaga
     4441 acgatcatcg gccagcgggg acgctgtctg aagcccttgc ggcgaacctc ctcttgaatg
     4501 cgtcgtatgt ccgtgaacac ggtgtcaaag gcggcagcca tttgttgatg gacatctttc
     4561 ggatcgtcgc cttccacaca gtgcggcacg tacccgtacc cgcggaacaa ggattcgagt
     4621 tcctcctgcg gcaggcgagc gagcacggtg gggccggcaa tcttgtagcc attgagatgg
     4681 agaatcggta gcacggcgcc gtcgcgcaca gggttgagga acttgtttga gtgccagctc
     4741 gtggccagcg gcccggtctc ggcctcgcca tccccgacca cgcaggccac aatcagatcg
     4801 ggattgtcga atgccgctcc gtaggcatgg gacaacgcgt acccgagttc acccccttcg
     4861 tgaattgacc ccggggtttc gggtgcaacg tggctgggga tgccgccggg aaaggagaac
     4921 tgtttgaaga gcttcttcag ccccgcctca tcctgcgaca catcgggata cacttcgctg
     4981 taggtgccct ccaggtaggt gttggccact agaccaggcc cgccgtggcc gggaccggtg
     5041 atatacatga cgttcaggtc cctctccttg atcacgcggt tgaggtggac gtaaatcaga
     5101 ttcaatccgg gcgtcgtgcc ccagtggcca aggagccggg gtttgatgtg ctcgcgcctg
     5161 agcggccgtt tgagcaaggg attgtcgtac aggtaaatct gccccaccga cagataattg
     5221 gcggcccgcc aatacgcatc catcttccgc agcagatcag gggacagcgg cgcggccttg
     5281 gttcttctcc tgttcatgat ctgtatccct ttcaagggaa agccccacaa tccgacagac
     5341 cgtcttcgcg atgatccatt cctcgtcggt ccgaatgacg cgcactgtgg cggcactacc
     5401 gtccgcggag atcaccggcg cgctcgtgcc gtttcgtttt ccatccagtg tgaacccgag
     5461 gaactcgagg ccccgacaaa tgcgtgcgcg cacctccggc gcgttttcgc ctatccctcc
     5521 ggcgaagatc agcgtgtcta ggcctcccag tgcggcggcc atggccgcaa tccacttctt
     5581 ggcttgatag caaaacagcg ccacggcttc ggcggcgcgg atgtccgtgc tctcgcgccc
     5641 gagcaactcc cgcatgtcgg agctgctctc cgagacgccg agcaatcccg actcgtgatt
     5701 gaccatgcgg tgaaactgct tggccgtcag gccctcggtg cgcgccagat accagccgag
     5761 ccccggatcc agatctccag tgcgagtgct catgggcatg ccggccgtgg gcgtaaagcc
     5821 catgctggta tcaatactct tgccctcgcg cacggcggtg atgctcgccc cgttgccaag
     5881 gtgcgccaga acgacgcgcc cgttcgcggt ccccgcgccc gccaggcggg ccagctcctc
     5941 gatcagatac gcgcaggaga gcccgtgaaa tccgtagcgg cgaactcccc ttgcatcgta
     6001 gcgccgcggg atgggcagca gacgtgccac gcgcggcatc tcgtgatgga aggccgtgtc
     6061 aaagcaggcg atctgcggta ggctcggaaa gcggcgatgg aacgtctggg ccagcaagat
     6121 ctcttccggc agatgctcgg ggtcgaaggc actgagttgc tgcaacgtct ccagcatctc
     6181 cggcgtgatg ggttgcggtt cccaatacct ggggccgcca tgcaccaccc tgtgaccgac
     6241 cgccgtcaca gcgctgcgct cgattcgttc ctggacccag tccatcagca ggctgactgc
     6301 cgttccatgc tccggggcca cgactcgccg cgagaaggtg tcgcccgggt tcaggccttt
     6361 ggccgcaaaa ctcccctgcg gaagccctat gccctcgatt ctgccctgca ggatccgccg
     6421 cagcctcccg tccgcttcga acaacgcgaa cttgatactc gaggagccgc cattcaccgt
     6481 aagcacgcct gtgcttccgg actgcgcgcg ccccgtatcc ccggaccgtt cccgacgcac
     6541 aggcatctac accttggctg tgagcgcggc gctcttggaa ccaatcaccg ccaggaggtc
     6601 attccaggat ttcacgaatg atttggcccc cccatcctgg agctgggcgg ccaaggcgtc
     6661 gatgtcgatc ccggccttga cgaactgggc caacatgtcc tcacagtcac cgccgtccgg
     6721 tggcaagggc gctccgatct cgccatggtc ggcgagggct gtcagggtgc cttccggcat
     6781 ggtgttcacc gtgaacggcg cggccagggc cttgatgtac aggacatcgg aggcctgggg
     6841 gtccttggtc cccgtgctgg cccacgggag acgctgcggg cgggcaccgg cattgtaggc
     6901 gcgctgccag cgtggcgact ccagcagcgt gcggtacgcc ttgtacgtgc gcttggccat
     6961 ggcaatgccg agctggttgc gaagcgtcgc ggggaccttc ccggcgacgg cggcgtccca
     7021 gcggctgacg aacagcgagg ccacggagcc cacacccggg ttcagcccga gggcgatgcg
     7081 ccgctcgatg ccgcgcagga acgcctcggc agccgccaga tagtgctccc gcgagaacag
     7141 gagggtcacg ttcaccggta ccccggcgaa gatcgccccc tcgatggccg gcagaccctc
     7201 ccgggtgccg gggatcttga tcaagacgtt cggctccccc gcccgggcgt gcagactctt
     7261 ggccgccgcc agggtcttct ccgtgttgta ggccagtaac ggcgagacct ccagcgatac
     7321 ccagccgtcc acgcgattcg tcctctcgtg gaccggccgg aacagatggg cggcctgggt
     7381 gagatcctcc agggccacct cgaagaacag gtcttctccc gatgtcccct tggcgagctt
     7441 cccgtggatg acggcgtcgt aggcggtgct gttcttgatg gcttggtcga agattgtcgg
     7501 attcgaggtg agtcccgtca ccgaaagctc gtcgatgtag cggcggagcg tcccgctcgt
     7561 tagcaggtcg cgtgtgatat tgtcgagcca gaggctctgg cccagatcgt ggagcagttg
     7621 agtggctttc attggtgacc tccggaaata atgaggttgg ctggcgtcat ggcaacgcct
     7681 gccgtcgcgc ctcgtgatgc cggcgccaac ggatcgagtc acaactcgac atggctcgtg
     7741 tcattccaga atcgaatcac cggccgggga aggccgtcct catagcccag cgcactcagg
     7801 ctcgccgtgc ccagcaggaa gtatcggccg gcccccggtg gaagccccac ccagcgggcg
     7861 gccaacaccc ggaggaaatg cccgcttgaa aagagcagcg catccccccg agcctcacgc
     7921 acacggctca cgacccggtc tgcccgcgtt ccaacctgct ccggcgtttc cccaccagga
     7981 cacccatcgc ggaacagttg ccagtcgggc cgttcggcac ggatctcgat cgtgcggcgc
     8041 ccctcgtact ccccgtagtt ccattcgacc agatcgcggt ccattgcggc ctctgcccca
     8101 aagccggcca aatcgcaggt ccgggccgcg cgctgcaacg gactggtcag caccgtggcg
     8161 aaggtccgtc cctgcagtcg ttttccgagg gagcgggcgt tgcgctcgcc gcgctcggtc
     8221 aagggaagat cggtcagccc cgtgtgctgt cccgagaggc tccaggcggt ctcgccgtgc
     8281 cgggccagat aaatgacagg aagggactcg ctcatgcggg tgttccttta ggggaattcg
     8341 ctttggccgg cgcccgatca cctcgggccg ccgcccaacc ttccaaccac gcgtgagccg
     8401 cctcaatgtc cgccgggatg agcccgtgtc ctccctgctc ccagtggagc gtgacgcttg
     8461 ccccggcctg gaccaggagt cgtcgcagcc gctctggctc gtccgggcgg acgatcgagt
     8521 cccgccgtcc ggctcccaga aagaccggcg tcccccggag gtccggcagc gtctccggga
     8581 cgaggggaac catcgcgcgg aagaggaccg cccccgcgag aacccttgga cggaggagca
     8641 ggacgctggc cgcaatgttc gccccgttgg agaagccgac cgccaggatg cgatcggagg
     8701 cgaggcggta ccgccgggcg gcggcctcga cgaagtcggc aagctcgtgg gtgcgcgccc
     8761 gcaggtcgtc caggtcgaac accccctcgg agagccgccg gaag
//
LOCUS       DATIFQ010000112        16271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_993539, whole genome shotgun sequence.
ACCESSION   DATIFQ010000112 DATIFQ010000000
VERSION     DATIFQ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993539"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VLE22_04720"
     CDS             <1..1397
                     /locus_tag="VLE22_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB13738.1"
                     /translation="PRCILVLADMLGSESRRKFRSRRAAIISFIYLVAAGTWIAVSDF
                     LLAHWVPLSDLPVYQTYKGWLFVAITTVGLYWLTRNAFHSIESSEQALLATNRTLRRT
                     EDRFRRAVLHAPFPIIIHAEDGEVLQLNNSWTELSGYTIDEIPTVSEWVEKAYPANKE
                     DVRRGIGDLYRLDRRVDQGEFTVTTKTGATRSWEFSSAPLGKLPDGRRAVISMAVDVT
                     ERKRLEAQFLQSQKMEAVGRLAGGVAHDFNNLLTIIIGHSENLLAQFGPDTPARVAAA
                     EIKGAGERAAALTRRLLAFSRRQPVQLRVVDVNGVIAGMESMLRRLIGEHILLETSPC
                     PDLWLVKADPCQLERVVMNLVINARDAMSNCGRIRIETRNLTAEDGGYWRKLALPPGE
                     HVLIAVHDSGKGIDPNILPNIFEPFFTTKEHGSGLGLSTVYGIVRQSGGEVRVESRPR
                     EGASFYVCLPRAGA"
     gene            1451..2374
                     /locus_tag="VLE22_04725"
     CDS             1451..2374
                     /locus_tag="VLE22_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HSB13739.1"
                     /translation="MSSAEEKRLDILCVADLCVDLVLTGNVRPHFGQFEQLIGDYALE
                     LGGSANLFASQFVKLGGRAGVIGRSGCDPFGDLALARLQSLGVDVARVRRDEDLKTGI
                     GVALSEPSDRAILTFLGSIDAVEPADLTEECLDSARHWHLASYFLLSRLRTVWPGWLR
                     RCKEQGLTTSLDTNWDPAARWDSVTELLPWTDVFLPNDAEALAISGESDVEAAGRRLA
                     SFGPLVVVKRGADGVAAFSGDKCWSEPATGHSARVVDTTGAGDNFDAGFLRAWMLGRD
                     VPECIELGQRCAFASLHAAGGIEGQLCEEIQ"
     gene            2388..3440
                     /locus_tag="VLE22_04730"
     CDS             2388..3440
                     /locus_tag="VLE22_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSB13740.1"
                     /translation="MTELLQDILAEPAELAGILDRLLGPQRRELDSAAALIGGARHVY
                     VAGIGSSWHAGMAVCSFFDAVARPALLAEASELLHFGAIPPDSALLVLSRSGRSVEIV
                     KLVRKAREAGAKIIGVTNTPDSPLARESDVVISLDARFDHLVSVSMYSGVALAGALAA
                     SAALGSLGDATAAALRASLVAAGESIDGWRRRIDESEWPGPGAPIYLLARGASLASCH
                     EARLAFEEATKSPASALTTGGYRHGPQESIHEGLRIALWLDGAKMRSEDIALAEDSRR
                     AGAHVMLIGQNIPADAGDLVVNLPAILPEWQFLIDIIPAQLAAECLARRKGVDCDSFR
                     LCPYIIESEGGLSARS"
     gene            3478..4299
                     /locus_tag="VLE22_04735"
     CDS             3478..4299
                     /locus_tag="VLE22_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB13741.1"
                     /translation="MTRREFGKIAAALPLACGAGLAAKRIPVGLQLYSVRKDCERDLP
                     GTLSAISKMGYEAVEFAGYYKRSAKELRKLLDDNGLKCCGTHTALETVLGAELPKTIE
                     FNQTIGNRNLIVPWLDHDKYLKPKSNLIKTAKMFSEIAAKLKPQGMRVGYHNHNVEFQ
                     PMDGSTPFNIFFENSSKDVCVQVDVGNALMGGGDPVSVIRRFPGRSISIHMKDWKPGE
                     DATLFGQGNVNWKDVFQACETVGGTEWYIIEYESETEPPIPSVKKCLDAFHAMRS"
     gene            4437..5498
                     /locus_tag="VLE22_04740"
     CDS             4437..5498
                     /locus_tag="VLE22_04740"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSB13742.1"
                     /translation="MSRTRSAAALGFRPTAIFVAIVISFGLTAGCRSGRSAGARTLRN
                     VSYDPTRELYQDINDAFAEKWKRETGESVTIEQSHGGSGKQARSVIDGLDADVVTLAL
                     AFDIDAIVERGKLIAPDWRNRFPNGSAPYTSTIVFVVRKGNPKGIKDWNDLSKPGISV
                     ITPNPKTSGGARWNHLGAWGYALRKFGGDEKRAREFVSALYRNVPVLDSGARAATTTF
                     AQREIGDVLLSWENEAFLAIREFGAGKLEIVVPSMSILAEPPVAVVDAVVERHGTRRL
                     AEEYLRFLYTEPAQEIAARHFFRPRSALALVKSGRKFPELDLFTVDEIAGGWRKAHET
                     HFADNALFDQIYRPVTQSE"
     gene            5498..6331
                     /gene="cysT"
                     /locus_tag="VLE22_04745"
     CDS             5498..6331
                     /gene="cysT"
                     /locus_tag="VLE22_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HSB13743.1"
                     /translation="MLARRYSIVPGFGLTFGYTVSYLSLIVLIPLAGLFVQSSAMTWQ
                     QFWQSISDARVVASYRLTILCALTAAGLNAVFGFLVAWCLVRYRFPGRRIIDAFIDLP
                     FALPTAVSGIVLTTVYSPQGWIGSLLAPLGIKVAYTPLGIIVALVFIGLPFVVRVVQP
                     AIQDIDPELEEAASSLGASRMQTFLRVLLPMLVPAISSGAALAFARALGEYGSVIFIA
                     GNMPMRTEITSLLIVTRLEQYDYAGATAIAVVMLTASFVMLLTINVLQWWNSRRYRSV
                     P"
     gene            6328..7236
                     /gene="cysW"
                     /locus_tag="VLE22_04750"
     CDS             6328..7236
                     /gene="cysW"
                     /locus_tag="VLE22_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HSB13744.1"
                     /translation="MSVTAQPAAIASAAGTPPVSREEPAWVRRTLLAIAFAFLILILF
                     VPLAAVFTEALRKGIGPYFNALLEPATLSAIRLTLLTAAIVVPLNAVFGLSSAWLITH
                     FEFRGRNLLLTLIDLPFAVSPVISGLIYVVLLGSHSVLGGWLAEHGVRIIFAVPGIVL
                     ATLFVTFPFVARELIPLMQEQGSEDESAAVTLGANGWQTFWRVTLPRVKWALLYGLIL
                     CNARAIGEFGAVSVVSGHIRGLTNTMPLHVEILYNEYDFVGAFAVASLLAMFALATIG
                     AKSLIEWRLRRESSLAVEVQRVEQAV"
     gene            7233..8309
                     /locus_tag="VLE22_04755"
     CDS             7233..8309
                     /locus_tag="VLE22_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HSB13745.1"
                     /translation="MNIALENVTKTFGTFRALQDVCLDVKDGELLALLGPSGSGKTTL
                     LRIIAGLDVPDHDGKSVIRFDGKQVDWSAAQQRLVGFVFQHYALFRTMTVFENIAFGL
                     RVGPREMRGSEADIRKKVNDLLSLIQLESMADRYPSQLSGGQRQRVALARALAIEPKI
                     LLLDEPFGALDAKVRQGLRRWLRRLHDEIHVTSVLVTHDQEEALEVADRVVIMNAGRV
                     EQAGTPEEVFHSPASAFVMDFLGNVNLFHARMDGDAVRPASADGASAAEGEAAGKAAR
                     ILVRPHDLDISVVENGASVLRARVLRIQTAGSMVKVEVVGDSGENVVVEIPHERFRSL
                     GIARDDLVFVSLKDSSLFVEDYSI"
     gene            complement(8360..9676)
                     /locus_tag="VLE22_04760"
     CDS             complement(8360..9676)
                     /locus_tag="VLE22_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13746.1"
                     /translation="MAKRYFSVFVLAFLLLACERRPDERAAGHKTDHSAGVLNARWHP
                     SGFELGDDNYNAVTAASDGKIYYVICAHKIDTGAQMYSYDPAAGKVQHLGDLTEASGE
                     KDLKAVPQGKSHVPFFEHAGKLYFATHLGYYNTQGGKEMVGVPPPGYKPYPGGHFLAY
                     DMKAGTFERLATAPAGEGIITMTMDPKRGRLYGITWPSAHFLSYDLAKKELKDWGLVS
                     EQGEKGSGPTFRVVCRSLALDPTTGSVYFTVSTGEILQYDYSLNALNKLEGCTMKRDV
                     FGVLDPDKPGHMGYNWRWTVWHDGEKVFYGTHGNTGYLFRFDPRARQIEVLDRIASAK
                     TRASGMYDEFRYGYMGLTLGPDGQTLYYLTGTPAGDEIRLVTYHIPTKKYSDHGAIVL
                     EDGSRPTWAQAIALGPDKRIYTVSKLRQDGKQKIDLLSFPDPLQGY"
     gene            complement(9735..10697)
                     /locus_tag="VLE22_04765"
     CDS             complement(9735..10697)
                     /locus_tag="VLE22_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13747.1"
                     /translation="MLKTVLLALALVMTASAAVKIEKVAYQGWPNCYRVSNGEIELIV
                     TSDIGPRIIRLGFVKAQNFFKEFKEMMGKSGEKQWMIRGGHRLWAAPEDSVKTYALDN
                     SPIKIDIKGDVLIATQPVEQLTGLEKQITVKMAPAGTAVEVVHRIRNTNRKAVELSPW
                     GLTVMAQGGVGFTGFPPRGKHPEVLPPTNPLVMWAYTDFSDKRWTFTKKYLALRQDPK
                     AAEPQKVGHFNSDTWGAYLLGSELFIKRYQADPSKTYPDFGCSYETFTNADFLEIETL
                     GPMTKLASGASVGHVERWSLHRNVRIPSLTDAVLDKVLLPLLGK"
     gene            complement(10735..12474)
                     /locus_tag="VLE22_04770"
     CDS             complement(10735..12474)
                     /locus_tag="VLE22_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSB13748.1"
                     /translation="MHNLDLIVTLAGGFIGALALGYLTHRLGWSSIVGYLLAGILVGP
                     HTPGFVANTQMAEQLAEVGVILLMFGVGLHFHLKDLLAVRGIAVAGALVQSTVATVLG
                     ALVFHAYGWNWSQGIVFGLALSVASTVVLIRVLSDNGALQSPTGRIAVGWLVMEDIFT
                     VFVLVLLPVLFSASSAQGSRGVPMAVGIAAAKLAVFIGFTLFVGSRLIPRLLNKVADT
                     HSRELFTLSVLAVALGIAVGSAYFFDVSMALGAFLAGMVVGQSEFSARAASEALPLRD
                     AFAVMFFLSVGMLFDPRQAFESPLLIALTLAIVMIGKPLAAVIIVAALGYSSKIGLGV
                     ALALAQIGEFSFLLATLGRQVGALPDGAMNPIVAVAIISIMLNPLVYRWVGPMEGFIE
                     RHPVLWRALNRRAVRERDSSLVYQEERSPAHRAVIVGYGPTGQMVSRILRQRGIEPTV
                     IEMNIETHRRLRGEGQAVVYGDANQREVLEQAGIANAASLILSASGTAASTEAVRVAR
                     EINPDIHVVARADFLRDTELLRRAGAAEVFSGEGEVALAIADSILRQLGATPDQLDEA
                     REQIRSDLRPTNS"
     gene            12719..13042
                     /locus_tag="VLE22_04775"
     CDS             12719..13042
                     /locus_tag="VLE22_04775"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HSB13749.1"
                     /translation="MAELPKVAEEAHDLELVTVLETEDSVALGLAKAALEEAGIEYLV
                     SSDEALRQLIRGGFGMSPDQAPYQTCRIQVAAETEAEARALLEPLAHPDPEAGAEEAP
                     GDEGW"
     gene            13133..13876
                     /locus_tag="VLE22_04780"
     CDS             13133..13876
                     /locus_tag="VLE22_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13750.1"
                     /translation="MGITVSEYVSGGYYVTRLSRHAEYMSPELIPDKVISASGCICEF
                     FPDDWAIEWTSESVDDRSRQAAALGISTIDLPKVVTWATESFSKAFGWPSAFYTLEAA
                     QEARARFLSDDSEIVIFGLGLHDSDVEDFLRAAKPEPTKPGCAPVGETGAFQCVSGGK
                     KIAAGGDAVGFELLATYTGLLTCSWLCNGLEKDCANELGIVTNRHGFVQTYADALRCA
                     EFISRDETGAEPGLWLPWLVTVYSTQSVE"
     gene            complement(13995..14906)
                     /gene="thiL"
                     /locus_tag="VLE22_04785"
     CDS             complement(13995..14906)
                     /gene="thiL"
                     /locus_tag="VLE22_04785"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HSB13751.1"
                     /translation="MNELRMVERIRRLVPGVARSGVVLGIGDDCAVYRPRGSREDLLF
                     TTDMVIEDVHFRRDSHPARAVGWRTLARGLSDIAAMGGEPRFCLVSLALAPWTDTRWL
                     NAFYRGFLDLARRAGAVLAGGDLARAKKVACDVVVCGAAPRGQALRRGGARPGDAIYV
                     SGELGASALGLERRSGAAWRRHLRPEPRLELGRFLRTRLRATAAMDLSDGLSLDLRRL
                     CLASGVAASLDGPLPSFPGVTLEQALHGGEDYELLFTVRPGTRVPEWHRGVPLTRIGT
                     ILRGASGRITYAGFPLPPLGWDHLRKR"
     gene            complement(14884..>16271)
                     /locus_tag="VLE22_04790"
     CDS             complement(14884..>16271)
                     /locus_tag="VLE22_04790"
                     /inference="COORDINATES: protein motif:HMM:NF023945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13752.1"
                     /translation="YYIGIGGGPGGEKARAVCLVPRGAEDGAFLELDREDLQLVANRP
                     VSFRLYSSLTRTGDTLGDVVEFDSAQRGDEVHVHAPLHAVIRVGKRTGDRLVPVKLAA
                     RLTEVGTLEIWCESKVSEHRWRLQFELRKTAVETEKPRAAAVVSEEAVAGAAEIVRTA
                     FSTAEPPLAPEELPARVEQAMGLGRNSWPLGVIRHLADVFLETAEGRRRSPAWEARWL
                     NLCGFCLRPGFGYPGDDWRIEQARRVYAAGLQFASHVQNEIEWWIFWGRLAGGMNRNQ
                     QIDVYQRLSPALLPRGGKRPRINSSLLREMWRAAASLELLPLQTKTDLGEELLRRVRA
                     GDYRESELWCLSRLGARQLFYGPINQVLPPATATRWVETLLKIAQARDTVVAIARRTG
                     DGARDLAPATIEMVRRSVENDPASGRLLAALEGEQPLGESEFSRIFGEELPGGLVFGN
                     SEESGERTTDG"
BASE COUNT         3027 a         4901 c         5260 g         3083 t
ORIGIN      
        1 gtccccgatg tatactcgtc ctagccgaca tgctgggctc ggagtcgcgc aggaagttcc
       61 gtagccgtcg agcggcaatc atcagcttca tctacctggt ggccgccggg acgtggattg
      121 ccgtctccga ctttctgttg gctcattggg ttccgctctc cgatcttccc gtctaccaga
      181 cttacaaagg ctggcttttc gtggcgatca cgacggtagg actctactgg cttactcgta
      241 acgccttcca ctccatcgag tcgtcggaac aggcccttct ggctaccaac cggaccctcc
      301 ggcgcaccga ggaccgcttc cgccgcgccg ttctccacgc cccgttcccg attatcatcc
      361 atgccgaaga cggcgaggtc cttcagctca acaattcctg gaccgagctc tccggataca
      421 ccatcgacga aatcccgacc gtctcggagt gggttgagaa agcgtacccc gccaataagg
      481 aagacgttcg aaggggaatc ggagacctgt accggctcga ccgccgggtg gaccagggtg
      541 agttcacggt taccacgaag accggcgcca cccgctcctg ggagttcagc tccgcgccgc
      601 tcggaaagct cccagacggc cgccgagccg tcatcagcat ggccgtcgac gtcaccgaac
      661 ggaagcggct cgaggcgcag tttctccaat cacagaagat ggaagccgtc ggcaggctgg
      721 ctggcggcgt cgcgcacgac ttcaacaact tgctgaccat catcatcggt cacagcgaaa
      781 acctgctagc ccagtttggc ccagacacac cggcgcgagt agccgcggcg gagatcaaag
      841 gcgccggcga gcgtgccgcg gcgctcacgc gccggctcct tgcctttagc cgccgccagc
      901 ctgtccagct cagagttgtc gacgtgaacg gcgttatcgc cggaatggag tccatgctcc
      961 gccggctcat cggggaacac attctgctcg aaacctcacc ctgccccgat ctctggctcg
     1021 taaaggccga cccgtgccaa ttggaacggg tcgtgatgaa cctcgtcatc aacgcccggg
     1081 atgcgatgtc gaactgcggc cgcatccgca tcgagacccg gaacctaacc gcagaagacg
     1141 gaggttactg gcggaagctg gcattgcccc ctggcgagca tgttctgatc gcggtgcacg
     1201 actccggtaa aggcatcgac ccgaacatcc tgcctaacat cttcgagccg tttttcacca
     1261 cgaaggagca cggctccgga ctcgggctct ccaccgtcta tggcattgtg cggcagagcg
     1321 gcggcgaggt ccgcgtggaa agccggccca gagagggcgc gagcttctac gtgtgcttgc
     1381 ctcgcgccgg cgcctgatcc gcggttccac ctccggtcgt atactgtgaa ctcacattca
     1441 ggcgccgccg atgtcctctg ccgaagaaaa acgcctcgac attctttgcg tcgccgacct
     1501 ctgcgtggat ctcgtgctca ccgggaacgt acggccgcac ttcggtcagt tcgagcagtt
     1561 gatcggcgac tacgcgcttg aacttggcgg ttcggccaac ctgttcgcaa gccagttcgt
     1621 gaagctcggc ggccgagccg gtgtcatcgg ccgctcgggc tgcgatcctt tcggagacct
     1681 tgcgctcgcg cggctccagt cgctcggtgt ggacgttgcg cgcgtccggc gggatgagga
     1741 tctcaagacg ggcatcggtg tggcgctgtc cgaaccttcc gatcgggcga ttctgacctt
     1801 tcttggcagc atcgacgcgg tcgagccggc cgacctcacc gaggagtgcc ttgattcggc
     1861 ccgccactgg cacctcgcgt cgtacttcct cttgagccgc ttgcggactg tctggccggg
     1921 gtggctccgc cgctgcaagg aacagggcct gacgacctcg ctcgacacga actgggaccc
     1981 cgccgctcgt tgggacagcg tcacggaatt gctcccctgg accgacgtct ttcttcccaa
     2041 cgacgccgag gcgctggcca tctccggaga gagcgacgtg gaagccgccg gacggagact
     2101 cgcttccttc gggcctctgg tcgtcgtcaa gcgcggcgcg gatggggtgg cggcgttttc
     2161 cggagacaaa tgttggagcg agccggccac cggccattcc gcccgcgtcg tggacacaac
     2221 cggcgcggga gacaactttg acgccgggtt tctccgggcg tggatgctcg gacgagacgt
     2281 tccggaatgc atcgaactgg gccagcgttg cgcctttgcg agcctgcacg ctgccggcgg
     2341 catcgaaggc cagttgtgcg aagaaattca atagaaaggg ccacatcttg accgaactgc
     2401 tccaagacat cctggccgag cccgcggaac tcgcgggaat cctagaccgc ctcctcggcc
     2461 cccaacgccg cgagctcgac agtgcggcgg ccctcatcgg cggcgcgcgc cacgtgtacg
     2521 tcgccggcat cggcagcagt tggcacgccg gtatggcggt ctgttcgttt tttgacgccg
     2581 tcgcgcggcc ggcgttgctc gccgaggcct cggaattgct ccacttcggc gcaatcccgc
     2641 cggattcggc tctgctggta ctctcgcgaa gcgggcgtag cgtggagatc gtcaaactcg
     2701 tccggaaggc gcgcgaggcg ggggcgaaga tcatcggcgt caccaacact ccggacagcc
     2761 cgctggctcg agaatccgac gttgtgattt cgctggacgc ccggttcgac cacctcgtct
     2821 cggtttccat gtattccggc gtggcactcg ctggggcgct ggctgcctcc gctgcgcttg
     2881 gctcactagg cgacgcgacg gctgcggcac tgcgcgcgtc gctagtggcg gcaggtgaat
     2941 cgatcgacgg gtggcggcgc cggatcgatg agagcgaatg gccggggccg ggcgctccta
     3001 tctacctgct ggcccgcggc gcgagcctcg cgagttgtca cgaggcaagg ctggcgttcg
     3061 aggaggcgac gaaaagtccg gcgtcggcac tgaccaccgg tgggtaccgc cacggtccgc
     3121 aggaatcgat ccacgaggga ttgcggatcg cactttggct cgacggcgcg aaaatgcgga
     3181 gcgaagacat cgcgctggcc gaggattccc gccgtgcagg cgcgcatgtc atgctgatcg
     3241 gccagaatat ccctgcagat gcgggcgatc ttgttgttaa cctgccggcg attctgcccg
     3301 agtggcagtt cctgattgac atcatccccg cgcaactcgc tgccgagtgc ctggcgcggc
     3361 ggaagggtgt ggattgcgac tcgttccgcc tgtgcccgta catcatcgag agcgagggcg
     3421 ggctcagcgc gcggagttaa gatggtgatc cggacgctat tctctttagg aggactcatg
     3481 accagacgcg aattcggaaa aatcgccgcc gcgctgccgc tcgcatgcgg cgcggggttg
     3541 gcggcgaaac ggatcccggt cgggcttcag ctttattcgg tgcggaagga ttgcgagcgc
     3601 gatctgccgg gcacgctctc ggcgatctca aagatgggct acgaagccgt cgagttcgcc
     3661 ggctattaca agcgcagcgc gaaggaattg cgtaagctgc tcgacgacaa cgggctgaaa
     3721 tgctgcggca cgcacaccgc cctggagacc gtgcttggcg cggaactgcc gaagacgatc
     3781 gaattcaacc agacgatcgg caaccggaac ctcatcgtgc cgtggctcga ccacgataaa
     3841 tacctcaagc cgaagagcaa cctcatcaaa acggcgaaga tgttcagcga gatcgctgcg
     3901 aagctgaagc cgcagggtat gcgggttggc taccacaacc acaacgtcga gttccagccg
     3961 atggacggct ccacgccatt caacattttc tttgagaatt cgagtaagga cgtgtgcgta
     4021 caagtggacg ttggcaacgc gctgatgggt ggcggcgacc cggtctcggt gattcgccgc
     4081 tttccggggc gttcaatctc gatccacatg aaggactgga agcccggcga ggacgccaca
     4141 ctatttggcc agggcaacgt caactggaag gatgtgttcc aggcgtgtga gaccgtgggc
     4201 ggcaccgagt ggtacatcat cgaatacgaa agcgagaccg agcccccgat cccgtccgtg
     4261 aaaaagtgcc tcgacgcctt ccacgccatg cgaagctaag tgcgcggggc cagaaagtac
     4321 attaactcaa acaatcaaaa cgaatttacc gagttgaggt tcaggcggcg aatggtaaca
     4381 ccactatgtc tatagagaat tggatatatc agtgttacca tggtcgtttg accccaatgt
     4441 cccgaacacg ctctgctgca gctctcggct tccggccaac ggcgattttt gtcgcgatcg
     4501 tgatttcgtt cggtctgaca gcgggctgcc gatctgggcg ttccgcggga gctcggactc
     4561 ttcgtaatgt ctcctacgat ccgaccaggg agctgtatca ggatatcaac gacgcctttg
     4621 cggagaaatg gaagcgcgag actggcgaat cggtcacgat cgaacagtca cacggaggga
     4681 gcgggaaaca ggcgcgttct gtcatcgacg ggctcgatgc cgatgtggtc acgctggccc
     4741 ttgccttcga catcgacgca atcgtggaac gtggcaaact gatcgcgccg gactggcgaa
     4801 acaggtttcc gaatggcagc gcgccttaca cgtcgaccat cgtgtttgtt gtccgaaaag
     4861 gaaaccctaa aggcatcaag gactggaatg acctgagcaa gccgggcatc tccgtcatca
     4921 cgccgaatcc caagacgtcc ggcggcgcac ggtggaatca cctcggcgct tggggctatg
     4981 cgctgcggaa gttcggcggc gacgagaaac gggcgcggga atttgtcagc gcgctttatc
     5041 gaaacgtgcc ggttctcgac tcgggagcgc gcgccgcgac aaccaccttt gcacagcggg
     5101 agatcggcga cgtcctcctg agttgggaaa atgaggcgtt cctggcgatc cgagagtttg
     5161 gagctggcaa actggagatt gtggttcctt cgatgagcat cctcgccgaa ccgcccgtgg
     5221 ccgtggtcga cgccgtggtg gaacgtcatg gcacgcgccg ccttgcggaa gagtatctcc
     5281 gctttctgta cacggagccc gcgcaggaga tcgcggcgag gcatttcttc cgcccccgct
     5341 cggcactggc cctggtgaaa tcaggcagga agttccctga gttagacttg ttcaccgtgg
     5401 atgagattgc cggtggctgg cgaaaggcgc atgagacgca tttcgcagac aacgcgctgt
     5461 tcgatcagat ttacaggccg gtgacgcaaa gcgagtgatg ctggcaagac gctacagtat
     5521 tgttccgggt tttggcctga ccttcgggta cacggtttcg tacttgagcc tcatcgtcct
     5581 cattccactg gcgggccttt tcgtgcagtc ctcggcgatg acgtggcagc agttctggca
     5641 atcgatttcg gatgctcgcg tcgtggcgtc gtacaggctc acaatcctgt gtgctttgac
     5701 ggcggccggt ttgaacgcgg tgttcggctt ccttgtggcc tggtgtctcg ttcggtaccg
     5761 gttcccggga cggcgaatca tcgacgcatt catcgatctt ccgttcgcgc tgccgacagc
     5821 cgtctccggc atcgtgttga caacagtgta ctcgccgcag ggatggatcg gcagtttgct
     5881 ggctcccctt ggaatcaaag tcgcctacac gcctctggga atcatcgtgg cgctggtgtt
     5941 catcgggctg ccgttcgtag tgcgcgtggt ccaacccgcg atccaggaca tcgatccgga
     6001 gctggaggag gcagcatcga gcctcggcgc cagccgaatg cagacgttcc tgcgcgttct
     6061 ccttccgatg ctcgtccccg caatcagctc gggcgctgcg ctggcctttg cgcgcgccct
     6121 cggggagtac ggatccgtca ttttcatcgc cggtaacatg ccgatgcgaa cggagattac
     6181 ctctttgttg attgtgacgc ggctcgagca atacgactac gcgggtgcaa cggcgatcgc
     6241 ggtcgtcatg cttaccgcat ccttcgtcat gctcctcacg atcaacgtgc ttcagtggtg
     6301 gaactcgcgc cgttaccgga gcgttccatg agtgtcaccg cgcagccagc agccatcgca
     6361 tcggccgcgg gaaccccgcc ggtctctcgg gaggaacccg cctgggtccg gcgaacgctg
     6421 ttggcgattg ccttcgcgtt cctcatcctg atcctctttg ttccgctcgc ggccgtgttc
     6481 acggaggcat tgcggaaagg aatcggccct tacttcaacg cccttctgga accggcgact
     6541 ctctccgcta tccggctgac gcttctgacg gcagccattg tcgtgcctct gaatgcggtc
     6601 tttggcctct cttccgcctg gctgatcacg catttcgagt tcaggggccg gaatctgctg
     6661 ctcacgctga tcgacctgcc ctttgccgtg tcgcccgtca tctcaggcct tatctacgtc
     6721 gtcttgctgg gttcgcacag cgttctgggc ggctggctgg cggagcatgg agtccgcatc
     6781 atcttcgccg tccccggcat tgtactcgca actctctttg tgacttttcc ttttgtcgcc
     6841 cgcgaactga tcccgttgat gcaggaacag ggtagcgagg atgaatcggc cgccgttacc
     6901 ctgggagcca acgggtggca aacgttttgg cgtgtcactc tgccgcgcgt aaaatgggct
     6961 cttctctacg gtctcattct gtgtaacgct cgcgcgatcg gggaattcgg agcggtatcg
     7021 gtggtgtccg gacacatccg cggcctgaca aacacaatgc ctcttcacgt cgagattctc
     7081 tacaacgaat acgacttcgt cggcgccttt gccgtggcgt cgctgctggc gatgttcgct
     7141 cttgcgacga tcggcgccaa aagcctgatc gagtggcgcc tccggcgcga aagtagtttg
     7201 gcggtggaag tccaacgggt ggagcaggcc gtatgaatat cgccctggaa aacgtaacca
     7261 agacgtttgg gactttccgc gccttgcagg acgtctgcct cgatgtgaag gacggcgagt
     7321 tattggcgct actgggacct tccgggtcgg gtaagacaac actgcttcgg atcatcgccg
     7381 ggctggatgt gcccgaccac gatggcaagt ccgtgatccg gttcgacggc aagcaggtgg
     7441 attggagcgc cgcacaacag cggcttgtcg gattcgtatt ccagcattac gcgttattcc
     7501 ggacgatgac ggtgtttgag aacattgcat tcgggctgcg tgtgggcccg cgcgagatgc
     7561 gcgggtccga agcggacatc cggaagaagg tcaatgacct gctttcattg atccagctcg
     7621 agtccatggc tgaccgatat ccttcgcagt tgtcgggcgg acagcgccag cgggtcgcgc
     7681 tcgcccgtgc cttggcaata gaacctaaga tcctgctgct ggacgagcct ttcggtgcgc
     7741 tcgacgccaa ggttcggcaa gggctccgga gatggctgcg gcggctgcac gacgagattc
     7801 atgtcacgag cgttctcgtg acgcatgacc aggaagaggc gctcgaggtg gctgaccgcg
     7861 tagtgatcat gaacgcaggc cgagtcgaac aggccggtac gcccgaggag gtgttccaca
     7921 gccccgcctc cgccttcgtt atggatttcc tcggcaatgt gaacctgttc cacgcaagga
     7981 tggatggtga cgcggtgcgg ccggcaagtg cggacggtgc atccgccgct gaaggggagg
     8041 ccgccggcaa agcggctcgt atcctcgtcc ggccgcatga tctggatatc agcgtcgtcg
     8101 aaaacggcgc atcggtgttg cgcgcccggg ttcttcgtat tcagaccgcc ggttcgatgg
     8161 tgaaagttga agtggtcgga gactccggcg agaacgtggt agtcgagatt ccgcatgagc
     8221 gctttcgctc gcttgggatt gcacgagacg acctggtgtt cgtcagcctg aaggactcaa
     8281 gtctcttcgt cgaggactac tcgatttagg cggcagacga caagaaccga tcgtctgcct
     8341 ccacgattga gccgacgcat cagtaaccct ggagagggtc ggggaaactg agaaggtcga
     8401 tcttctgctt tccgtcctgc cggagtttcg agaccgtgta gatacgtttg tcgggaccca
     8461 gagcgatggc ctgcgcccag gtcggccggc tgccgtcctc cagcacgatc gctccatgat
     8521 cggagtactt cttcgtgggg atgtggtagg tgaccagccg aatctcgtcg ccggcgggcg
     8581 tcccggtcag gtagtacagc gtctgaccgt ccgggcctag cgtgagtccc atgtagccgt
     8641 accggaactc gtcatacatg ccgctggcgc gcgttttcgc cgaggcgatg cggtcaagca
     8701 cttcaatctg acgggcgcgc ggatcgaagc ggaaaagata gccggtattc ccgtgcgtac
     8761 catagaagac tttctcgcca tcgtgccaca ccgtccagcg ccagttgtag cccatgtggc
     8821 cgggtttgtc cggatcgagg actccaaaga cgtcacgctt catcgtgcac ccttccaact
     8881 tattgagcgc gttcagactg tagtcgtatt ggagaatctc gccggtggaa accgtgaagt
     8941 aaaccgagcc ggtcgtcggg tcgagcgcga gcgatcggca gacgacgcgg aaggtcggcc
     9001 cggagccctt ctcaccctgc tcggaaacga ggccccagtc cttcaactcc ttcttcgcca
     9061 ggtcgtagga caggaagtga gccgagggcc aggtaatccc gtagaggcgg ccgcgtttcg
     9121 ggtccatggt catggtgatg atgccctcgc cggcaggtgc ggtggcgagt cgttcgaagg
     9181 tcccggcctt catgtcatag gccaagaagt gcccgccggg gtagggtttg tagcccggcg
     9241 gcggcacgcc caccatctcc ttgcccccct gcgtgttgta gtagccgaga tgggtggcaa
     9301 agtagagctt gcctgcgtgc tcgaagaacg gcacgtgaga cttgccctgc gggacggctt
     9361 tcaggtcctt ctcgcccgac gcctcggtga ggtcgccgag gtgctgcacc ttccctgccg
     9421 ccggatcata agaatacatc tgcgcgccgg tatcgatttt gtgagcgcag atgacgtagt
     9481 agatcttgcc gtcgctggcg gcggtcaccg cgttgtagtt gtcgtcgccg agctcgaatc
     9541 cggaagggtg ccatcgggcg ttgaggacac cggccgagtg atcggtcttg tgtccggcgg
     9601 cccgttcgtc aggccgccgc tcgcaggcga gcagcagaaa agcaagcacg aagacggaga
     9661 agtatcgttt ggccatgggt gggtccatca tacgcaaaac gggcctccgt ttcccgggag
     9721 ctgctggggc gcggctattt tccgagcaga ggcagcagaa ccttgtccag gacggcgtcg
     9781 gtcagcgaag gaatccggac gttcctgtgg aggctccagc gctcgacgtg cccgacggaa
     9841 gcgcccgagg caagtttcgt catcgggccc agcgtctcga tttcgagaaa gtccgcgttg
     9901 gtgaacgtct cgtaggagca gccgaaatcc gggtacgtct tcgagggatc ggcctggtag
     9961 cgcttgatga atagctcaga gccgagcagg taggcgcccc aggtgtcact gttgaagtgg
    10021 ccgactttct ggggctcggc cgccttcgga tcctggcgca gcgcgaggta ctttttcgtg
    10081 aaggtccagc gcttgtcgga gaagtcagtg taggcccaca tgaccagcgg gttggtcgga
    10141 ggaagaacct ccgggtgctt gccgcgcggg gggaaaccgg tgaagccgac accgccctgc
    10201 gccatcaccg tgagtcccca gggagacagc tcgaccgctt tgcggttcgt gttgcggatg
    10261 cggtgaacga cctcgacggc ggtcccggcg ggggccatct ttacggtgat ctgtttctcg
    10321 agtccggtga gttgctcgac gggctgggtg gcaatgagta cgtccccttt gatgtcgatc
    10381 ttaatcgggc tgttatcgag ggcgtaagtt ttgacggagt cctcgggagc ggcccagagt
    10441 cggtggccgc cgcggatcat ccactgcttt tctccgctct tgcccatcat ttccttgaac
    10501 tccttgaaga agttctgtgc tttgacgaag ccaaggcgga tgatgcgggg accgatatcg
    10561 ctggtaacga tgagctcgat ctccccgttg gagacgcggt agcagttggg ccagccctgg
    10621 taggcgacct tctcgatttt cacagcagcg gaagcggtca taacgagtgc gagtgcaagc
    10681 aggacggttt tcaacatcga cgctattatg tcgcaattcc ggagggccgg cacgctagct
    10741 gtttgtagga cgaagatccg atcgaatctg ctcacgagct tcgtccagtt gatctggagt
    10801 tgccccgagt tgacgaagga tgctgtccgc gattgccaga gcgacttcgc cttcgcccga
    10861 aaagacctcg gccgctccgg cgcggcgcag caactccgta tcccgaagga aatcagctcg
    10921 cgcgaccaca tggatatcgg gattgatctc gcgggcgacc ctcacagctt cggtcgaggc
    10981 agcggtgccc gaggcgctca ggatcaggct cgccgcgttg gcaatgcccg cctgctccag
    11041 aacttctctc tgatttgcgt cgccatatac caccgcctgg ccctcgccac ggagtcggcg
    11101 gtgcgtttca atgttcatct cgatcacggt cggctcaatt ccgcgttgcc gcaggatgcg
    11161 ggacaccatt tgtccggtcg gtccatatcc cacaattacg gcgcgatggg ccgggctgcg
    11221 ctcctcctgg tacacgagcg aggaatcgcg ttcgcgtaca gcccgtcggt tgagcgcccg
    11281 ccacagcacg ggatgacgtt cgatgaagcc ttccataggt cccacccatc gataaaccag
    11341 cggattcagc atgatggaga tgattgcgac cgccactatc ggattcatcg caccgtccgg
    11401 gagcgcgccg acctgccgtc cgagcgtcgc tagaaggaat gagaactcgc cgatttgcgc
    11461 aagcgcgagc gcgacgccga gtccaatttt ggagctgtat ccgagcgccg caacgatgat
    11521 gacggcggcg agcggcttgc cgatcatgac gatggcgaga gtcagggcaa tgagcagtgg
    11581 agactcgaag gcctggcgag ggtcaaacag catccctacc gagagaaaaa acatcaccgc
    11641 gaacgcatcc cgcagcggca acgcctcgga ggcggcgcgt gcgctgaatt ccgactgtcc
    11701 tactaccatg cctgcaagga acgcgcccaa ggccatcgaa acgtcaaaga aatacgcgga
    11761 accgacggcg attccgagcg cgactgcaag cacgctgagg gtgaacaact cacgggaatg
    11821 tgtgtcggcg accttgttca agaggcgcgg gatgaggcga ctgcccacga aaagggtaaa
    11881 acctatgaag actgcgagct tggccgcggc gattccgacc gccatcggca cgccgcggga
    11941 tccttgggcg gaggacgcgc taaagaggac cggcaggagg acaagcacaa aaaccgtaaa
    12001 gatgtcttcc atcaccagcc agccgacggc gatccgtccc gtgggactct ggagtgcgcc
    12061 attatcggag agtacgcgaa tcagaacgac cgtactcgca acggagagag ccagcccgaa
    12121 aacgatgccc tgggaccagt tccacccgta ggcgtgaaac acgagagcac ccaggacggt
    12181 cgcgaccgtg ctttggacca gagcgcccgc caccgcgatt cctcggacgg cgagcaggtc
    12241 cttcaggtgg aaatgtaggc cgacgccgaa catcaagagg atcacgccga cttcggccaa
    12301 ttgctccgcc atctgggtgt tggcgacgaa acccggcgta tgaggaccga cgagaattcc
    12361 cgcaagcaag tatccaacga tggacgacca ccccaggcgg tgggtaaggt agccaagtgc
    12421 cagcgccccg ataaaaccgc cggcgagcgt tacgatgagg tccaaattgt gcacgtttgc
    12481 agtctctgtt ccgcggtcca gacacttttc gtcgccaagg cgaattttca gagtaacaaa
    12541 gcggggcgtc tggctccgcc ggcacggcgg tcagaggccg tgggtggaag ttcggtggag
    12601 tattctgtaa gccagcagcc gccgtagcgc ggcgtggagg tggagcgtgt attgtccacg
    12661 gtgccgtacc gagtatcggc agggagtttc cgagtgcgcc gactgtgggg cgccgctcat
    12721 ggcggagctt ccgaaggtgg ccgaggaagc gcacgacctg gagcttgtga cggtgctgga
    12781 gacggaggac tccgtggcgc tggggctggc gaaggccgcg ctcgaggagg cggggatcga
    12841 atacctcgtg agcagcgatg aagcgttacg acaattgatc cggggaggat ttgggatgag
    12901 tccggatcag gcgccgtacc agacgtgccg gattcaggtg gcggccgaaa ccgaggccga
    12961 ggcccgcgcg cttctggagc cgctcgcaca tcccgacccg gaagccggag cggaggaggc
    13021 accgggcgac gaggggtggt gaaggctgtg agcggcctta caaggccgag gtctgccctc
    13081 cgcccgcaaa atggttgaga gcgatcgcgc aaagaatgtc caaagtgaag agatgggaat
    13141 taccgtgagc gaatatgtgt ccggtggcta ttacgtaact cgcctctcaa gacacgccga
    13201 gtacatgtcc ccggaattga tccctgacaa ggttattagc gcatccggtt gtatatgtga
    13261 gttctttcca gacgactggg ccattgagtg gacctccgaa agcgtcgatg accgatcgcg
    13321 gcaagcagcg gcgctcggca tttccacaat cgaccttccg aaagtcgtga cgtgggcaac
    13381 ggaatctttt tcaaaagcgt ttggatggcc cagtgctttt tatacactag aggcggcaca
    13441 ggaagcgcgt gcgaggttcc tgtcggatga ctccgagatt gtcatcttcg gtctgggcct
    13501 gcatgactcg gacgtcgagg actttctgag ggcggcgaag ccggaaccca caaagccagg
    13561 gtgcgccccg gtaggagaga cgggcgcctt ccaatgcgtc agcggcggga agaagatcgc
    13621 agccggtggt gatgccgtgg gtttcgaact cctcgcaaca tacactggac tgcttacttg
    13681 ctcgtggctg tgcaacggtt tggagaagga ttgcgcgaac gagctaggca ttgttacgaa
    13741 tcgacatgga ttcgtccaaa cgtacgctga cgcgttgcgc tgtgctgagt tcatttcgag
    13801 agatgagacc ggagcagagc caggcctgtg gcttccttgg cttgttacag tctactccac
    13861 ccaatccgtc gaatagaaag cgtgcttgtg gtttgggaac gtccctttcc cagattacgc
    13921 agacggaggt gggcgactgc tccaccgggg gacgagctgg tatcagcgag gagcggagtt
    13981 ccaggtcgct gccgtcagcg ctttcgaagg tggtcccagc cgagaggggg gagggggaaa
    14041 ccggcgtagg tgatgcggcc ggacgcgccg cggagaattg tgccgatgcg cgtgagcggg
    14101 acgccacgat gccactcggg gacgcgtgtc ccggggcgca cggtgaagag aagctcgtag
    14161 tcctcgccgc cgtgcagcgc ctgttccaga gtgacgccgg ggaacgacgg cagcggacca
    14221 tcgagcgagg cggcgactcc ggaagcgagg cagaggcggc gaagatcgag cgagaggccg
    14281 tcgctcaagt ccatggccgc tgtggcgcgc aagcgcgttc gaaggaagcg gcccagttcg
    14341 aggcgcggct cgggccgcag gtgccgcctc catgcggcgc cggaccgtct ttcgaggcca
    14401 agcgccgaag caccgagctc gcccgagacg taaatggcgt caccgggacg agcgccgccg
    14461 cgacggagcg cctgtcctct aggagcggcg ccgcagacga cgacgtcgca ggcgactttc
    14521 ttcgcacggg cgaggtcgcc gccagccagc accgcgccgg cgcgacgggc gagatccagg
    14581 aacccacgat agaacgcgtt cagccatctc gtatccgtcc agggcgcgag agcgagggag
    14641 acaagacaga agcgcggctc gccgcccatg gctgcgatgt cgctgagacc gcgcgcgagc
    14701 gtacgccatc cgacggcgcg ggcggggtgg ctgtcgcggc ggaaatgcac gtcctcgatc
    14761 accatgtcgg tggtgaaaag aaggtcttcg cgggaacctc gggggcggta gacggcgcag
    14821 tcgtcgccga tgccgagcac gacgcccgat cgggccacgc ccgggacgag ccggcgaatc
    14881 cgttcaacca tccgtagttc gttcacctga ctcctccgaa ttgccgaaga cgaggccgcc
    14941 gggcagttcc tcgccgaaga tccgactaaa ctcgctttcc ccaagcggct gttccccttc
    15001 gagcgccgcg agcagacggc cgctcgccgg atcattttcc actgagcggc ggaccatctc
    15061 aatggtagcg ggggccaggt cccgggcgcc gtctccggtg cgccgtgcga tggcgacgac
    15121 ggtgtcgcgc gcctgagcga tcttgagcag cgtttccacc cagcgagtcg cggtcgcggg
    15181 cggcagcacc tggttgatcg ggccatagaa caactggcgc gcgccgaggc gcgagaggca
    15241 ccacagttcg ctctcgcgat agtcacctgc gcgcacgcgc cggagtaatt cttcaccgag
    15301 gtcggtcttg gtctgaagcg gcaacagttc gagactggcg gccgctcgcc acatctcccg
    15361 cagcaggctg ctgtttatgc gcgggcgctt gccgccgcgg ggaaggagag caggcgagag
    15421 gcgctggtag acgtcgatct gctgattgcg gttcatccca ccggccagac gtccccagaa
    15481 gatccaccac tcgatctcgt tttgcacatg gctggcgaac tgcaagccgg ctgcatacac
    15541 gcggcgggcc tgctcgatgc gccagtcgtc accgggatag ccgaaccctg gccgcaggca
    15601 gaagccacag aggttgagcc agcgggcttc ccaagcgggc gaacggcggc gcccctcggc
    15661 cgtttcgagg aagacatcgg ccagatggcg gatgacgccg agcggccagg aattccgccc
    15721 gagtcccatc gcctgttcga cccgggcggg aagctcctcg ggcgcgagtg gcggttcggc
    15781 agtggaaaac gcggtgcgaa cgatctcggc ggcacccgcg acagcctctt cgctgaccac
    15841 cgccgcggct cgcggcttct ccgtctcgac ggctgtcttg cgcagctcga actggagccg
    15901 ccagcggtgc tcgctaactt tcgactcgca ccagatttcc aaggtgccga cctcggtgag
    15961 gcgggcggcc agtttcaccg gcacgagccg gtcgccggtt cgctttccga cgcggattac
    16021 ggcgtgaagt ggtgcgtgaa cgtgcacctc atcgccgcgc tgcgcggaat cgaactcaac
    16081 gacgtcgccg agcgtgtcgc cggtgcgcgt cagcgagctg tagaggcgga acgaaacagg
    16141 ccggttggcg acgagctgaa ggtcttcacg atctagttcg aggaaggcgc cgtcttcggc
    16201 cccgcgcggc acaagacaga cggcccgcgc cttctccccg cccggaccgc cgccgatgcc
    16261 gatgtagtat g
//
LOCUS       DATIFQ010000113        14901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2718464, whole genome shotgun sequence.
ACCESSION   DATIFQ010000113 DATIFQ010000000
VERSION     DATIFQ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14901
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2718464"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VLE22_04795"
     CDS             <1..294
                     /locus_tag="VLE22_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13753.1"
                     /translation="GTVVEVKVNGKPAGIIGWQPYELDVSRFVRGGDNTVEVTVIGSL
                     KNLLGPHHGKISRGFVSPWLFRNAPEHMPPGSGYDLDSYGLFGDIELIEYRPR"
     gene            302..1366
                     /locus_tag="VLE22_04800"
     CDS             302..1366
                     /locus_tag="VLE22_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB13754.1"
                     /translation="MPGYDESDINRLLKTFRHQEADRVPHLEFWVTSKPVYEYVLGYP
                     LQYDVVDARVGGQSITPEDHVLFARKLGMDAVACNFSWRPNNVFQKAADGTEHYTGGT
                     VRTRDDFSRLEPPPALDDQLRYLERYLRAAEGTGVGVFANFTSFFDSALLAIGVTDSM
                     YLFHEDRPFLESLMDILLDHQEGVVRAVLGRFAADLAFVLVNDDIAYNSGLLIRRDMF
                     LEMFPHRMKRLIAPVKERGKLLAMHTDGRMEKILPILSDIGFDIVHPIEPEVNDIFEL
                     KQQWAGRLAFVGNIPTPLLAYGTKERIEETVREYCRRLGPGGGWVLGSSTSIMDGIPP
                     ENFVAMTRAVHKYGRLGADS"
     gene            1363..2454
                     /locus_tag="VLE22_04805"
     CDS             1363..2454
                     /locus_tag="VLE22_04805"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13755.1"
                     /translation="MTRRDFCALLPFAGAAAPARIKAFCVDFNWRRPRNPGWHNDFAR
                     PGHWADASPAEHVAWYEALGANVIQTFCVSCNGYAWYKNGFVPQQPGLKHDFLPEMVE
                     LGHRKGMLVLGYFCAGANTKWGQDHPELSYGSPSTLHIPFTDEYLDYLCRSITDAVRR
                     TKIDGYMLDWVWPPDDKLREKGWSDCERKLYEKLTGKTFPVGGRPGAAEKLAYERAAI
                     ERCWARVREATKKASPDCIIWLTCNNLTDSRVIDTRMLREADWVLNEAPNAEYYARAR
                     KMVGPQTRLIQCLVGWPGHDARAYLFDRSRGDNDLYGFAEPGDNSLPRPVSEYLSKPV
                     SAFTGTDPKAANDRNIAALARFYRGLPVD"
     gene            complement(2451..3527)
                     /locus_tag="VLE22_04810"
     CDS             complement(2451..3527)
                     /locus_tag="VLE22_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HSB13756.1"
                     /translation="MKRLLFALFLLSGALLAAGPKAKNVILFLTDGGGLATVSAASIH
                     GYGRPQALYIQNMLHIGLSDTSSASNWVTDSANGMTAIVTGVKTHNGVISQGPDAVRG
                     VKDGTPLKTILEYAEERGLSTGVMTDVEIGDATPAVCYAHHNDRGKMGEIFLQLTKPR
                     FGDGPEIVIGRGRDGLARGTQALGVDLWAALRKAGYTLLETPDAIKSADTAAPKLAGV
                     FKDKFDVTEAVDLAINRLSRNRKGFFLMVECDVHLGDARKSVEAMVAADRLVRHTAEK
                     MKGTDTLIISTSDHSYDLRLPGGVKKGEDIIPHMKLEGHHDGEEVVVAAQGPGSELVH
                     GFLPNTRIFEIMMAAYGWKADARR"
     gene            3617..4891
                     /locus_tag="VLE22_04815"
     CDS             3617..4891
                     /locus_tag="VLE22_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB13757.1"
                     /translation="MNSRRTFVLSAGGAVARMAAGKAQANDRIRIAVIGVNGRGRDHI
                     EGFEPLPDAEVAMLCDPDRNVLFERAEQFEKKYGRKVATETDLRRVYENKEIDAVSIA
                     TPNHWHALAAIWACQAGKDVYVEKPGTHNVREGRKLIEAAHKYNRIVQHGVQLRSSEA
                     LQEAVGHLRKGTIGNVYMARGLVFRWRPSIGKKPNEQPPEYLDYNLWLGPAEERPFSR
                     RLVHYNWHWHWDFGNGDVGNQGIHETDMCMWGLGVQKLPSKITAMGGKFLFDDDKETP
                     ETLSSTYLFGDEKKMIEFEVRPWCTNLEDGAGVGNLFYGSEGYMVVKGYNEYAIYLGE
                     RRERGPARKAGGDHYANFLKAVRSRKTSDQNGPVETVHLASALAHLGNISYRLGRQLT
                     FDPAAEKFVGDQQANAMLTRNYRAPFVVPEKV"
     gene            complement(4912..7344)
                     /locus_tag="VLE22_04820"
     CDS             complement(4912..7344)
                     /locus_tag="VLE22_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB13758.1"
                     /translation="MSVRQHLEILAQDVCYALRGFFRAPMFTLAAVFAVAVGVGAGTA
                     VFSVVDRVLFRSLPYPAAERLVSFGMVAPIVPQEFMLAYDYLDWRTSQTPFESLGAWS
                     GVSDCDLNDTNPVRLRCAYVDASLLPALGVEPIRGRNFTAAEDRPNAPRAAIISHRLW
                     QSRFAGDPGAIGRPVPLDGQPTAVVGVLPPGFELPSLATADLLVPLALNEVEQRTRRT
                     ATLLFTVGRLRPGVTVPQAIAALQPLLEKSLESVSPGFRKEVKLRLRPLRDRQIQDSR
                     RASWILLASVLAVLLIACSNVANLLLARAAARRRELAVRAALGAGRGRLVRQALTESV
                     LLGVAGGAAGCVLASLLLQLFVAVAPDGIPRLNQADVDGRVLLFTLAVSLLSGALFGL
                     APALQNPSVEFLTGWRTLGPSHRLLRQGLVGMQICVSMVLLTGAGLLLRSLWNLQNQP
                     LGMRTDSVLTAEVTLGRKSYAEPARRLAFFEELEERLRRIPGVAEVALSDSLPPEGNG
                     MGTMLYAAIDVYGRPRIAEGTGGSVASRTVSPRYFAALGIPILRGRGFQDEDRDPNRN
                     VVILSDTLARRMFPGEDPLLKQIQPGRRGPWLSVIGLAGNVKNAGLIERDDPEFYMVR
                     KRSPINVRPGAVAIIRGALDPETFAESVRAKIASIDPTLPVNIRTMEQRVGKLAERPR
                     FNALLLGIFAAMGLLLAAVGLYGVVSFLVAQRTREIGVRMALGAAPAAITRDVLKRTV
                     RWAAAGAVAGVIGSIFATRLLQGMLYRVSAADPWTFTGALAVLMGVTLLAAWIPSRRA
                     ARVDPMEALRQE"
     gene            complement(7430..7957)
                     /locus_tag="VLE22_04825"
     CDS             complement(7430..7957)
                     /locus_tag="VLE22_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13759.1"
                     /translation="MKRRRVDGTRPERRHDPRGAAWLAVLIMTVAAGLPRPAVAQSEG
                     SWDNINRLRAGQKIQVVQMNLKSSEGKFLSSSDTAIALNVAGLDIAIPREDVMRVTLQ
                     ERPKRLRNALIGAAIGAGAGFALGYAVSAKEAGGEVELLSVALLATAAGAGAGASLPS
                     YPTLYRAPPPAGRRQ"
     gene            complement(8168..8590)
                     /locus_tag="VLE22_04830"
     CDS             complement(8168..8590)
                     /locus_tag="VLE22_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13760.1"
                     /translation="MCRRLLCVLTFAAGMIVLPLSAETKPNFSGTWKLNEKAQRADGP
                     RNIVFKIDHKDAVLKYTATGQTSGGEAFTEAVEVPIDGKEHPGIHSGTVSARWEEQRL
                     VIRYTISGNEYSMVLRSSADGNQMFRDVLGQHEVYDKQ"
     gene            complement(8817..9089)
                     /locus_tag="VLE22_04835"
     CDS             complement(8817..9089)
                     /locus_tag="VLE22_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HSB13761.1"
                     /translation="MALPAKRRAVFHPEFREDLRFWVKSDRKIALRILDLVEAALRDP
                     FQGAGKPEPLRYVLAGCWSRRITGEHRLVYRVSDDRIDFLQARYHY"
     gene            complement(9432..11894)
                     /locus_tag="VLE22_04840"
     CDS             complement(9432..11894)
                     /locus_tag="VLE22_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB13762.1"
                     /translation="MTSLLQDLRYALRLCRAKPGFTLMVLLTLALGIGVNVVMFTVVE
                     TALLRPLPYPQADRLVELTESSRDYPSIPVAYPNFQDWRRQSAAFENMSALALVSSTL
                     ADQAGARRIPAAQVSASFFDVLDLPPSMGRNFQASDDRTGAPTTAIVTHGFWLREMNG
                     DPAPIGKSIQLDRRPYTVIGVLPAEFRFYRESEIFLPIEAAMGNVLANRANHSEMVVL
                     GRLRTGITPERGRTEIRTIARRLEQQYPDTNSAISANLVTLRERVSGPARTSLLVLWG
                     AVCLVLLIACANIANLLLARSTERRRELAIRAALGAGRARLLRQLLIESLVLALAGAA
                     IGLLLVLWSLKAAAGLLPAGLGAADLRLDWAVLLYTLLATLACALLFSLVPAWRSARN
                     GVCEALKEGGRAGELREHGRLRDALVTAEVAVSLTLLVGAGLLGRSMYRLLHVDLGFQ
                     TDNLASVEVNVPFAFDFGRSQAFFAQVLERTQALPGVEAAAAATNFALAGGRSAVEVY
                     REDRPTPARGHFPSIPYQVASPGYFRTVGIPLRRGRFFDSRDRGPLIPPGTDPGRAFG
                     MLHETRLSAVINETMARTLWPGEDPIGRYFRFGTPELHGPGLEVVGVAGDTRQEGLET
                     RPQASWYLCSLQFPLINGSTHLLLRSQRPATALGAEVRRMIAGIDPQIVVPAARTFDE
                     IRDGVLSGRRTRTALLAMFAGLALLLALVGLYSVVSYAVAQRRHEFGVRMALGADQRK
                     LVRLVMRHSMRPVLAGVACGLAGAVALTRALRGMLFEVQPGDPLTLAAVTLLLVATAL
                     AASLLPARRAARANPLDAMRCE"
     gene            complement(12048..13736)
                     /locus_tag="VLE22_04845"
     CDS             complement(12048..13736)
                     /locus_tag="VLE22_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSB13763.1"
                     /translation="MCLLLLIPLLLSAQTAPWPTYGWSAAAPETQGLDPARLDQASQY
                     ITENCPTRYSLLVVRNGYLVFERYYNGSYASQANNIKSMSKSVLSVLTGIAIQEGRIR
                     GVGQTLEEFYPEYFHDGLDPRKSDITLKHLLTMTAGFEWDELSTTSAELFRSWNWRQK
                     MIEWPMANNPGEVFNYNTGLTHLLSGILTKVSGSSTLAYATSRVFDPLRITCGRWTQD
                     PMGIYFGGSEVWLTARDLAKFGLLVLRQGRWEDHEIVNREWLRESTRIHARNGLGWPM
                     GDYGYLWWNKPQQGYPVILASGFGGQYVFLVPDLDLMVVTTADSGISVENLIVYTQAF
                     DLLSRYILPAVAGPAPSIEPGGIVQSADYTPVLAAGSFGSVFGRALSLVENTWDGDMP
                     ADGRLPTSVGGVRVRIGGRTAAVSYAGPGQINFLIPPDLAPGRQQVEVLTARGGASAD
                     VEVADAAPAWFQTGRKRVRPGDVVELYASGLGRTEPAAPVGAVVREPLVLISPPEVLV
                     AGRAATVEWAGLVYAGVYQVNVRVPADTPPGEARVQLRVAGRSAASEAVLDVVQ"
     gene            13948..>14901
                     /locus_tag="VLE22_04850"
     CDS             13948..>14901
                     /locus_tag="VLE22_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB13764.1"
                     /translation="MSTVMKDVWFGLRILRRNPWFTAIAVLSLGLGIAVNTTVFSWID
                     SVLLHPFPGVGDPQELALIETVTPTGEYLVNTSVLDYLDYRDKLKLVSGVALGRFTPL
                     SVGADGKTARAWAELVSPNYFDLLKVKPVLGRGFLPEEVREEGGAPVAVISYGMWQAR
                     FGGDPKVLGKIIRLNRHELTIIGVAPREFHGSLAGVVFDVWVPATMATAMGTGDGTLH
                     YRGTRDQTSTIVRLKPGVTLEQVRAEVSALGKRLAELYPDTNRGIDLTVTPVWKGHLG
                     AQGMLLQPLRTLMAVSALLLLIVCANVANLLLARAVSRQKEF"
BASE COUNT         2781 a         4816 c         4791 g         2513 t
ORIGIN      
        1 gggaccgtcg tcgaagtcaa agtcaacggc aagcctgccg gcatcatcgg ctggcagccc
       61 tacgagcttg acgtcagccg cttcgtccgc ggcggcgaca acacggtcga agtgaccgtc
      121 atcggctccc tcaagaatct gctcggcccg caccacggca agatcagccg cggtttcgtt
      181 tcgccgtggc tcttccgcaa tgcccccgag cacatgccgc ccggctcggg ctacgacctc
      241 gattcctatg gcctcttcgg cgacatcgag ttgatcgaat atcggccgcg ttgacgtcca
      301 gatgcccggc tacgacgagt ccgacatcaa ccggctgctc aaaacattcc gccaccagga
      361 ggcggaccgc gtaccccacc tcgagttctg ggtcaccagc aagcccgtct acgagtacgt
      421 tctcggctac ccgcttcaat acgacgtcgt cgacgcgcgc gtcggcggcc agtccatcac
      481 gccggaagat catgttctct tcgcccggaa attgggcatg gacgcggtcg cgtgcaattt
      541 ctcctggcgc ccgaacaacg tcttccagaa ggccgctgat ggcactgagc actataccgg
      601 cggcacggtc agaacccgag acgattttag ccgcctcgaa ccgccgcccg cgctcgacga
      661 tcagttgcgc tacctggagc ggtaccttcg cgccgcggag ggaaccggcg tcggcgtgtt
      721 cgcgaacttc acttcgttct tcgacagcgc gctgctggcc atcggtgtca ctgattcgat
      781 gtatctgttt cacgaggacc gcccgttcct tgagtcgctg atggacatcc tgctcgatca
      841 ccaggagggc gtcgtccgcg cggtcctcgg ccgtttcgcc gccgacctcg cctttgtgct
      901 cgtcaacgac gacattgctt acaactccgg cctgctcatc cgccgggaca tgttcctcga
      961 aatgttcccg caccgcatga aacggctgat cgctcccgtg aaagaaagag gcaaactcct
     1021 cgccatgcac accgacggcc ggatggagaa gatccttccg atcctcagcg acatcgggtt
     1081 cgacattgtc cacccgatcg aaccggaagt gaatgacatc ttcgagctca aacagcagtg
     1141 ggccggaaga cttgccttcg tcggcaacat cccgacgcct ctgttggcct acgggacgaa
     1201 ggagcggatc gaggagaccg tgcgggagta ttgccgccgc ctcggtccgg gcggcggttg
     1261 ggtgctcggt tcctctacca gcatcatgga cggcatcccg ccggagaact tcgtcgccat
     1321 gacccgggcc gtccacaaat acggccgcct cggagcagac tcatgacccg gcgtgacttt
     1381 tgcgcactgc ttcctttcgc tggcgccgct gcgccagcgc gcatcaaagc cttctgcgtg
     1441 gacttcaact ggcgccggcc gcgcaacccc ggctggcaca acgactttgc caggcccggc
     1501 cattgggcgg acgcttcccc ggccgagcat gtcgcctggt acgaggccct cggcgcaaac
     1561 gtcatccaga ccttctgcgt ctcctgcaat ggctacgctt ggtacaagaa tggcttcgtt
     1621 ccgcagcagc ccggtctgaa gcacgacttc ctgcctgaga tggtcgaact gggccaccga
     1681 aaaggcatgt tggtcctcgg ttacttctgt gccggggcaa acacgaagtg ggggcaggat
     1741 catccggagc taagctacgg ctcgccctcc acgcttcaca ttccgttcac ggacgaatac
     1801 ctcgactacc tatgccgctc gatcaccgac gcggttcggc gcaccaaaat cgacggctac
     1861 atgctggact gggtctggcc gcccgacgat aagctccgag aaaaaggctg gtcggactgc
     1921 gagcggaagc tgtacgagaa actaaccgga aaaacgttcc ccgtgggcgg ccgtcccggc
     1981 gccgccgaga agctcgcgta tgagcgcgcg gccatcgaac gctgctgggc ccgcgtacgc
     2041 gaagccacga aaaaagcgag cccggactgc atcatctggc tgacgtgcaa caatctgacc
     2101 gactcccgcg tcatcgacac ccggatgcta cgcgaggcgg attgggtcct gaacgaggcg
     2161 ccgaacgcgg agtactatgc ccgcgcacga aaaatggtcg gcccgcaaac gcgcctgatc
     2221 cagtgcttgg tcggttggcc gggccacgac gcccgcgcct atctgttcga ccggtcgcgc
     2281 ggggacaacg acctttacgg cttcgcggaa cccggcgata attcactccc gcgtcctgtc
     2341 tccgagtact tgtcgaagcc tgtctcggcc ttcaccggca cggacccgaa agccgcaaac
     2401 gaccgcaaca tcgcggccct cgcgcgcttc tatcgcggcc tgccggtcga ctaacggcgc
     2461 gcgtcagcct tccagccgta ggcggccatc atgatttcga agatgcgcgt gttcggaagg
     2521 aatccgtgca ccagttcgga gccgggacct tgcgccgcca cgacgacctc ttcgccgtcg
     2581 tggtggcctt cgagtttcat gtgggggatg atgtcctcgc ccttctttac gccgcccggc
     2641 agacgcagat cgtaggagtg atccgacgtg ctgatgatga gggtgtccgt gcccttcatc
     2701 ttctccgcgg tgtgccgcac gagcctgtcg gctgcgacca tggcctcgac gcttttccgc
     2761 gcgtcgccga ggtgaacgtc acactcgacc atgaggaaaa agcccttgcg gttccgcgaa
     2821 aggcggttga tcgcaaggtc gactgcctcc gtgacgtcga acttgtcttt gaaaactccg
     2881 gcgagtttcg gagcggcggt gtcggcactc ttgatcgcgt ccggcgtttc gagcagcgtg
     2941 tagccggctt tccggagcgc cgcccagaga tctactccca gggcttgtgt gcccctcgcc
     3001 agaccgtccc ggccgcggcc gatcacgatt tccggaccat cgccgaatcg gggttttgta
     3061 agttggagaa aaatctcgcc cattttcccg cgatcgttgt gatgggcgta gcacaccgcc
     3121 ggggtagcgt ccccgatctc gacgtccgtc atcacgccgg tcgagagacc gcgctcctcg
     3181 gcgtattcga ggatggtctt gaggggcgtt ccgtccttga ccccgcgaac ggcgtcaggc
     3241 ccttgtgaaa tcacgccgtt gtgtgtcttc acgccggtga cgatggcggt catgccgttg
     3301 gcggaatcgg tgacccagtt cgaagcgctg gaggtgtccg agagtccgat gtgcaacatg
     3361 ttctggatgt agagcgcctg ggggcggccg tagccgtgaa tgctcgcggc gctgaccgtc
     3421 gccaggccgc cgccgtcggt caggaacaga atcacgtttt tcgccttcgg accggcggca
     3481 agcagcgctc cagagagcaa aaagagtgca aaaagtagtc gtttcatact caaaatgtgt
     3541 ccttttgcga caaagagtga ttctatctga acgtcggagt cgtccgtgat agaatcgccc
     3601 gcgaggactc attcccatga acagccgcag aacttttgta ttgagcgccg gtggggcggt
     3661 ggcccgcatg gcggcgggca aagcgcaggc gaacgaccgg atccgcatcg ccgtgatcgg
     3721 cgtcaacggt cgcggccgcg atcacatcga agggttcgag ccgttgccgg acgccgaggt
     3781 ggcgatgctc tgcgacccgg accgcaacgt gctgtttgag cgcgctgagc aattcgaaaa
     3841 gaaatacggc cggaaggttg cgaccgagac tgacttgcgg cgggtctacg aaaacaagga
     3901 gatcgatgcg gtcagcatcg ccacgccgaa ccactggcac gccctggcgg cgatctgggc
     3961 gtgccaggcg ggcaaggacg tctacgtcga aaagcccggc acacacaacg ttcgggaagg
     4021 acgcaaactc atcgaggcgg cgcacaagta caaccggatc gtacagcacg gcgtgcagtt
     4081 gcgcagctcg gaggcgttgc aggaggcagt cgggcatctg cggaagggca cgatcggaaa
     4141 cgtctacatg gcgcgggggc tggtgttccg gtggaggccg tcgatcggga agaaacccaa
     4201 cgagcaacct ccggagtacc tggactacaa cctgtggctg gggccggccg aggagcggcc
     4261 gttctcgcgg cggctggtgc attacaactg gcactggcac tgggatttcg gcaacggcga
     4321 cgtcggcaac cagggcatcc acgagaccga catgtgcatg tggggcctgg gcgttcagaa
     4381 gctgccgagc aagatcacgg cgatgggcgg taagttcctc ttcgacgacg acaaggaaac
     4441 gccggagacg ctgagctcga cgtacctgtt tggcgacgag aagaagatga tcgagttcga
     4501 agtgcgcccg tggtgcacaa acctcgagga cggcgcgggt gtgggcaacc ttttttacgg
     4561 ctccgagggt tacatggtgg tgaagggcta caacgagtac gcgatctacc tcggcgagag
     4621 gcgggagcgc gggccggcgc ggaaggcggg cggcgatcac tacgcgaact tcctgaaggc
     4681 ggtgcggagc cggaagacgt cggaccagaa cgggccggtg gaaaccgtac acctggcgtc
     4741 cgcgctggcg caccttggca acatctcgta ccggctgggc cggcagttga cgttcgaccc
     4801 cgcggcggag aaattcgtgg gcgaccagca ggccaacgcg atgctgacgc gcaactaccg
     4861 cgctccgttc gtagtgccgg agaaggtata gagtcccagc ctgagcgcga gttattcctg
     4921 gcgcagggcc tccatgggat cgacacgggc ggcgcggcgg gaggggatcc aggcagctag
     4981 cagagtgacc cccatcaaga cggccagcgc gccggtgaac gtccaggggt cggccgcgga
     5041 cacgcggtag agcatgccct gaagcagccg ggtggcgaag attgatccga tcacgcccgc
     5101 cacggcgccg gctgccgccc agcgtacggt gcgtttcagc acatcccgcg tgatggccgc
     5161 gggcgcggcg ccgagcgcca tgcggacccc aatctcccgg gttcgctgcg ccacaagaaa
     5221 tgagaccacg ccgtaaagac ctaccgccgc cagcagcagc cccatagcgg cgaagatccc
     5281 caacagaagg gcgttgaagc ggggccgctc cgccagtttc ccgacgcgct gctccatggt
     5341 ccggatgttc accggcaagg tgggatcgat cgacgcgatc ttcgcccgta ccgattccgc
     5401 gaaggtctcc gggtcgagag caccacggat gatcgcaacc gctccaggcc gcacgttgat
     5461 gggcgagcgc tttcgcacca tgtagaactc agggtcgtcc ctctcaatca gtccggcgtt
     5521 cttcacgttg ccggccaggc cgatcacgct cagccacgga cctcgccggc ccggctggat
     5581 ctgcttgagc agcgggtctt cgccggggaa catccgccga gccagcgtgt cactaaggat
     5641 gaccacattg cggttcgggt cgcgatcctc gtcctgaaag ccccggccgc gcaggatcgg
     5701 gattccgagg gcggcgaaat agcgcggtga gaccgtccgc gacgcgacgc tgccgccggt
     5761 tccttccgcg atgcgcggcc gtccgtacac gtcaatcgcg gcgtagagca tcgttcccat
     5821 gccgttgccc tccggtggca gcgagtcgct gagagcgacc tccgccaccc ctgggatccg
     5881 gcgaagtcgt tcttcgagtt cctcaaagaa ggccagccgc cgcgcgggtt ccgcatacga
     5941 tttccggccg agcgtgacct cggccgtcag cacactgtcc gtccgcattc cgagcggctg
     6001 attctggagg ttccaaaggc tgcgcagtag caggccggcg cccgtaagca ggaccatcga
     6061 cacacagatc tgcattccaa ccaggccctg acggagcagc cggtgactcg gcccgagcgt
     6121 tcgccagccc gtgaggaact ccacgctggg gttttgaagc gctggagcca acccgaacag
     6181 ggcgccggag agcagcgaca cggctagcgt gaacagcagc actctcccat ccacatcggc
     6241 ctgattcaac cttggaatgc cgtcgggggc gactgccacg aacaactgca acagcagaga
     6301 ggcaagcaca cagcccgcgg cgcctcccgc cactccaagg agtacactct cggtgagcgc
     6361 ctgccgcacg agccggccgc ggccggcccc gagggccgcg cgaacggcca gttcccgccg
     6421 gcgcgcagcc gcgcgcgcga gcagcaggtt ggccacgttg gaacaggcga tgaggagcac
     6481 cgccagcacc gaggccagca agatccacga ggcccgccgc gaatcctgga tctggcggtc
     6541 tcgaagcggc cgcagccgca gctttacttc cttgcgaaac cccggggaga cggattccag
     6601 ggatttctcc agcagcggct gcagcgccgc gatagcctgc ggcacagtca cgccgggtct
     6661 caagcgcccg acggtgaaga gcagtgtcgc agtccggcgt gtgcgctgtt ccacttcgtt
     6721 gagcgccaga ggcacgagga gatcggcggt ggccagggag ggcaactcga accccggggg
     6781 caggacgccc accaccgccg tcggctgccc gtccaggggg accggtctgc ctatcgcgcc
     6841 cggatcgcct gcgaaccggc tctgccagag ccggtgagaa atgatcgcgg ccctcggcgc
     6901 gttcggacga tcctcggcgg cagtgaaatt gcggccccgg ataggctcga caccgagggc
     6961 cggcagcagg gaggcatcga cgtaggcgca tcggaggcgc actgggttcg tgtcgttgag
     7021 gtcgcaatcg ctcacgccgg accaggcgcc gagcgattca aatggcgtct gtgatgtgcg
     7081 ccagtcgagg tagtcgtacg ccagcatgaa ctcctgggga acgatcgggg cgaccatgcc
     7141 gaaggagacg aggcgctccg ccgcgggata ggggaggctc cggaagagga cgcggtccac
     7201 gacgctgaag acggcagtcc cggcgccgac gccgacggcc accgcgaaaa ccgcggcgag
     7261 ggtgaacatg ggcgcgcgga agaagccgcg cagggcgtag cagacgtcct gcgcgaggat
     7321 ctcgaggtgc tgtctgacgg acatgcggag gcctcctccc gaggctctac cgcgtttaga
     7381 aaccgcggca agtcggaagt tcccgaaacc cgatgtgcgt gcagaagatc tattgccggc
     7441 ggcccgcggg cggtggcgcc ctgtagagtg tcggatagct cggtagcgag gcgccggctc
     7501 cggctccagc cgccgtcgca agcagagcca cgctcagtag ttcaacttct cctccggctt
     7561 ccttcgcgga aaccgcataa ccgagcgcga agcccgcccc ggcgccgatc gccgcaccga
     7621 tcagtgcatt ccgtaaacgc ttgggcctct cttgcagggt gactcgcatc acgtcttccc
     7681 gcggaatcgc gatgtcgaga ccagcaacat tcaaagcgat tgccgtgtca gaggagctga
     7741 gaaacttgcc ctcgctggac ttgagattca tctggaccac ctggattttc tgccccgctc
     7801 gtaaccggtt gatgttgtcc caggaaccct cggactgtgc caccgccggc cgaggcaggc
     7861 cggcggcgac ggtcataatc aagacggcca accatgccgc tccacgcgga tcatggcgcc
     7921 gctccggcct cgtcccgtca acgcgacgcc ttttcatgtt gttccctccc aaatccgagg
     7981 gagcaattcc ctcgccagga atcgacgccg cattccgttg aaaacgcacg gccttcaggt
     8041 gggcggatag gaagcccccg tcccccggct acggcctttt ccgacactgt catgcggaac
     8101 tgactggcac gaccgaacgc ggacttcgag gccgacgtcg agcacgcgtc acgaagccgg
     8161 cgcccggcta ttgtttgtcg tagacctcgt gctgaccgag cacgtcccgg aacatttggt
     8221 tgccatcggc ggacgaccgg agcaccattg agtactcgtt accgctgatc gtgtaccgaa
     8281 tgacaaggcg ctgctcctcc caacgcgccg acacggtgcc tgagtggatt ccgggatgct
     8341 ctttgccgtc tatggggacc tcgacggctt ccgtaaaagc ttcgccgccc gaggtctgac
     8401 ccgtagcggt gtatttcaag acggcgtcct tgtggtcgat cttgaacacg atgtttctgg
     8461 ggccatccgc ccgttgggct ttctcattca gcttccaggt gccgctgaag ttgggcttgg
     8521 tttccgcaga cagcggcaga acgatcattc cggccgcaaa agtaagaaca cacaacaatc
     8581 ttctgcacat cagcttgatt cctctttctg ggcgcgaacc gcaccctggc tttgcgccgg
     8641 atatcaggtc gacgtgttct gcgaatgctt ctggcgatcc tgttccggcg cgttccacca
     8701 agttagacac cggaggatgt gcattgtttc ggcggcgagg aggccttagc gatgctgagg
     8761 tgcaagtacc gcgttcggtg agtggcggcg gaaatgcaag gggcggctgt ccgccgtcaa
     8821 tagtggtacc gcgcctgaag gaagtcgatc cggtcgtcgc tgacccggta gaccagccgg
     8881 tgctctcccg tgattcgccg tgaccagcag ccggcgagca cgtaccgtaa cggctccggc
     8941 ttgccggctc cctgaaacgg gtcgcgcaac gccgcttcga ctaagtccag aatccggagc
     9001 gcgattttgc ggtccgactt cacccaaaag cgaaggtcct cgcggaattc ggggtggaaa
     9061 acggcacggc gtttagcggg cagagccaat cgcgatctcc cgccgcaatc gctccatcga
     9121 tgccggtctg ccttttccaa gttccgcacg cttcagtgct gttagcaatc tgcgtgcatt
     9181 tcggggagaa cgtaggaggt gggcagtttc catcaggccc gcaagttcgg ctgcagggag
     9241 catcgcgaca tcgctcgaac ctttccggcg gacgatcacg acctcctggt ccctgactac
     9301 gcggtccaga acacttgcga ggttggcccg gagactggta taagtcattt ccatcggcat
     9361 cagaggcacc tcgctttatt gtacaggatt cctgtacaat acatctgccg gcgtgaggga
     9421 cggcagagga tctattcgca gcgcatggcg tccagcgggt tggcccgcgc ggcccggcgg
     9481 gccggaagca ggctggcggc gagcgctgtc gcgaccagca gcagtgtcac cgctgccagg
     9541 gtgagtggat cgccaggctg cacctcgaaa agcatgccgc gcagtgcgcg cgtcagcgcc
     9601 accgctccgg ccagaccgca ggcgactccg gccagcaccg gccgcatact atgccgcatc
     9661 accagccgga ccagtttgcg ctgatccgcg ccgagcgcca tgcgcacgcc gaactcatgg
     9721 cgccgctgtg ctacagcgta ggatacgacg ctgtagaggc cgaccagtgc cagcaacagc
     9781 gccaggcccg caaacattgc cagcagcgcc gtgcgggtgc gccggccgga aagcacgccg
     9841 tcgcggattt cgtcgaaggt gcgggcggcg gggaccacaa tctgcggatc gatgccggca
     9901 atcatgcgcc gtacctcggc tcccagcgcc gtggccggcc gctggctgcg cagaagcagg
     9961 tgcgtgctgc cgttgattaa cgggaactgt agcgagcaca gataccagga tgcctgtggc
    10021 cgggtctcca gcccttcctg ccgagtgtct ccggccacac cgaccacttc caatccggga
    10081 ccatgcagtt cgggagtgcc gaagcggaag taccggccaa tcggatcctc gcccggccag
    10141 agagtgcgtg ccatggtctc gttgattacg gccgacaagc gggtttcgtg aagcatccca
    10201 aaggcgcgcc cgggatccgt tccgggtgga atgagaggcc cgcggtcgcg cgagtcgaag
    10261 aaacgaccgc gccgcagagg gattccgaca gtgcggaagt atccggggct ggccacctgg
    10321 tagggtatag atgggaaatg gccccgggcg ggcgtgggac ggtcttcacg gtagacctcg
    10381 accgcggacc tgccgccggc cagtgcgaag ttggtcgccg cagcggcggc ttccacgccc
    10441 ggcagggcct gtgtccgctc gagcacctgg gcgaagaacg cctgagaccg cccgaaatcg
    10501 aacgcaaacg ggacattcac ctcgaccgag gccagattgt ccgtctggaa gccgagatcg
    10561 acatgcagaa gccggtacat gctgcgcccg agcaggccgg cgcctaccag cagggtaagg
    10621 gagaccgcta cctcggcagt gaccagcgcg tcgcgaaggc ggccatgctc ccgtagctcg
    10681 cccgcgcgcc cgccttcctt gagggcctca cagacgccgt tccgggcact ccgccaagcg
    10741 ggaacgaggc tgaacagcaa ggcgcaagcc agcgtggcca gcagggtgta aagcagcaca
    10801 gcccagtcca ggcgcaggtc ggcggctcca aggccggccg gcagaagacc agccgccgcc
    10861 tttagactcc acaacaccaa cagaagccct atggccgcgc cggcgagggc caggaccagg
    10921 ctctcgatga gcaactggcg gagcaggcgg gcccgccctg cgcccagggc ggctcgtatg
    10981 gcgagttcac gcctgcgctc ggtcgagcgc gccagcagga gattggcgat gttggcgcag
    11041 gcgatcagca gcaccaggca cacggcgccc cagagcacca gcaagctggt gcgggccgga
    11101 ccgctgacgc gttcccggag ggtcaccaga ttcgcgctga tggcgctgtt ggtgtcaggg
    11161 tactgctgct cgagtctccg ggcgatggtc cggatctcag tgcggccccg ttccggcgtg
    11221 attcccgttc gcagccgccc caggacgacc atctcggagt ggttggcgcg gttcgccagt
    11281 acgttgccca tcgcggcctc gatgggtagg aagatctcgc tctcccgata gaagcggaac
    11341 tcggcaggga gcaccccaat cacggtatag ggcctccggt ccaactggat gcttttgccg
    11401 atcggggctg gatccccatt catctcgcgt agccagaaac catgggtgac gatggcggta
    11461 gtgggggctc ccgtccggtc gtcgctcgcc tggaagttgc ggcccatgga aggaggtaag
    11521 tccagaacat cgaagaagga cgcggagact tgggcggccg ggatccggcg tgcgccggct
    11581 tggtcggcca gggtactgct taccagcgcc agggcggaca tgttctcaaa cgcagccgac
    11641 tgccgccgcc agtcctggaa gttgggatac gccaccggga tggaggggta gtcacgggaa
    11701 ctttccgtga gttcgaccag ccggtccgcc tggggatacg gcagcgggcg gagcagggcc
    11761 gtttcgacca ccgtaaacat aacaacgttg acgccgatgc ccagggccag ggtgagcagg
    11821 accatgaggg tgaacccggg cttagcgcga cagagccgca gggcatagcg caggtcctgg
    11881 agcagagaag tcatcggcag acacagtcgc aagtcggctg ccaaagtgct aaggcattga
    11941 taggatgcga gccgcagccg ggaaacggtc ccgcaggcgg acactgccgt cccagaggcg
    12001 gacaggctgc cgggcggaat gcctccgtcc gccgcaccac gtcagcgcta ctgcacgacg
    12061 tccaacacag cttcgctcgc cgctgaacgt ccggcgactc gaagttggac gcgtgcctcg
    12121 ccgggcggcg tatcggcggg aacacgaacg ttcacctgat acacaccggc atacaccaat
    12181 cccgcccact cgacggttgc cgcacgcccg gccacgagca cctcaggcgg gctgatgaga
    12241 accagcggct cgcgaaccac tgctcccacg ggagccgcgg gttcggtgcg ccccagcccg
    12301 gacgcgtaca actcgactac gtctccgggc cgcacacgtt tacggcccgt ttgaaaccac
    12361 gccggtgcgg cgtcggcgac ttcgacatcc gccgatgcgc ctccgcgggc ggtgagaacc
    12421 tcgacctgtt gtcttccagg ggcgaggtcc ggcggaatca gaaagttgat ctggccgggt
    12481 ccggcgtaac tcaccgccgc cgtccgcccg ccgatccgca cccggacgcc gcccacagag
    12541 gtcgggagcc gcccgtcggc cggcatatcg ccgtcccacg tgttttcgac aagactcagc
    12601 gcgcgtccga acaccgagcc gaaggaaccc gccgccagaa ctggcgtgta gtccgccgac
    12661 tgcacgatgc ctcccggctc gatggacggc gcaggaccgg cgacagccgg cagaatgtat
    12721 cgcgagagga gatcgaacgc ctgcgtgtac acgatcaagt tctcgacgga gatcccggag
    12781 tccgccgtcg tcactaccat caggtccaga tccggaacga ggaatacgta ttgcccgcca
    12841 aagcccgatg cgaggatgac cgggtaaccc tgctgcggct tgttccacca caggtacccg
    12901 tagtcaccca tgggccaccc gagcccgttg cgcgcgtgaa tgcgcgtcga ctcgcgcagc
    12961 cactcccggt tcacgatctc gtggtcctcc cagcgccctt gccggagcac gagcagtccg
    13021 aacttcgcaa ggtcgcgcgc ggtcagccag acttccgagc cgccgaagta gatacccatc
    13081 ggatcctgcg tccaacggcc gcaggtgatt cggaggggat cgaatacccg tgaagtggca
    13141 taggcaagcg tgctcgagcc cgagaccttc gttaatatgc ccgagagcag atgagtgagg
    13201 ccggtgttgt aattgaagac ctcgcccggg ttgtttgcca tcggccactc gatcatcttc
    13261 tgacgccagt tccagcttct gaagagctct gcggaggtcg tcgagagctc gtcccattcg
    13321 aaacccgcgg tcatggtcag aaggtgcttg agcgtgatgt ccgacttgcg cgggtcgagg
    13381 ccgtcgtgga aatactccgg gtagaactcc tcgagcgttt ggccgacgcc ccgaatccgg
    13441 ccctcctgga tcgcgatgcc ggtcaggacg gaaagcacgc tctttgacat cgacttgatg
    13501 ttgttggcct gcgacgcgta gctgccgttg taatacctct cgaaaaccag gtagccgttg
    13561 cggactacca gtagactgta ccgcgtcggg caattctcgg tgatgtactg ggacgcctga
    13621 tcgaggcgcg cagggtcgag cccctgcgtt tcgggagctg ccgccgacca gccgtacgtc
    13681 ggccatggtg cggtctgcgc tgacagcaaa aggggaatga ggagtaaaag acacactcgt
    13741 ctgaaaccca tggtcagaga aatggacgca gcggcctcgc gtgaggttcc gagcccaacc
    13801 aacaggccgt ccgagggctt ccgggcgccg cgagcgaaaa atctcgattc cgaacaactc
    13861 ttcccacaag cggacagccg gcccagcgaa gtcgcgcgcc tgtaaggact tgcgattgga
    13921 attcgagttg caagccctct tatctctatg tccaccgtca tgaaggacgt ctggttcgga
    13981 ttgcggatcc tgcggcgcaa cccgtggttc acggccatcg ccgtcctgtc gctcggcctg
    14041 ggcattgcgg tgaacacgac ggtgttcagt tggatcgatt ccgtcctgct gcaccccttc
    14101 cccggcgtcg gcgatccgca ggaactcgca ctcattgaga ccgtgacccc cacaggggag
    14161 tatctggtca acacctccgt tctcgactac ctcgattacc gggacaaact gaagctggtc
    14221 tccggagtgg ccctcgggcg attcacgccc ttgagcgtcg gggccgacgg caaaacggcg
    14281 cgcgcctggg cggaactcgt ctcgccaaac tacttcgatt tgctgaaggt caagcccgtg
    14341 ttgggccgtg ggtttctgcc cgaggaggta cgcgaggaag gcggcgcccc ggttgccgtc
    14401 atcagctatg gcatgtggca ggcccggttc ggcggcgacc caaaggtgct gggcaagatc
    14461 atccgtttga accggcacga gttgaccatc atcggtgtgg cgccccggga gttccacggg
    14521 tcgctggccg gtgtggtctt cgacgtgtgg gtgccggcca cgatggccac ggcgatgggc
    14581 accggcgacg gtacactgca ctaccgcggg acgcgcgacc agacctccac gatcgtgcgg
    14641 ctgaagcctg gcgtcaccct cgagcaggtg cgcgcggaag tcagcgcgct aggcaaacgg
    14701 ctggcggagc tctaccctga caccaaccgg ggcatcgacc tcacggtaac accggtgtgg
    14761 aaggggcatc tcggcgccca gggcatgctg ctccagccgc tgcggactct gatggcggtc
    14821 tccgcgctcc tgctgctgat cgtctgcgca aacgtcgcca acctgctgct cgcccgcgca
    14881 gtatcccgcc agaaggagtt c
//
LOCUS       DATIFQ010000114         5574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2718563, whole genome shotgun sequence.
ACCESSION   DATIFQ010000114 DATIFQ010000000
VERSION     DATIFQ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2718563"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..1800)
                     /locus_tag="VLE22_04855"
     CDS             complement(421..1800)
                     /locus_tag="VLE22_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HSB13765.1"
                     /translation="MTTLFVQASYIIAAALFILALKWMSHPATSRRGVWAGEVGMLLA
                     VVGTLLRFEVVNYQWIFIAFLIGSAIGVPLAYLMPMTAVPQRTALSHAFGALAAALIG
                     VAEYYRETPHGFTMVALSIEVLLGFLTFTGSLMAFGKLQEVLPQRPIVYKGQNIFNLS
                     ILGAAVLFGVYLCIHPEATWAFPIFSILALIFGVLLIVPIGGADMPTVIALLNSYAGL
                     SASFMGFALNNYLLVIAGALDGSSGFILSVIMCRAMNRSFTNVLFGAFGQLQVKAHEV
                     EQRTARSATAEEAAQILDAARLVIVVPGYGMAVAQAQHKLREMFDLLTKRGVDVRFAI
                     HPVAGRMPGHMNVLLAEADVPYDKLFEMDEINSEFPQADVALVIGANDVTNPGARTDK
                     SSPIYGMPILDVDKARTVMVIKRSLNPGFAGIDNELYYMDKTLMLFGDAKAFLTEVNK
                     EIAAVGVSA"
     gene            complement(1797..2060)
                     /locus_tag="VLE22_04860"
     CDS             complement(1797..2060)
                     /locus_tag="VLE22_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HSB13766.1"
                     /translation="MLATFLGLELIKKVSPLLHTPLMSLTNAISAISVVGAILITGEA
                     HATNLGRVLGFIAVTAATINVVSGYLITDRMLKMFKKREPGKK"
     gene            complement(2199..3047)
                     /locus_tag="VLE22_04865"
     CDS             complement(2199..3047)
                     /locus_tag="VLE22_04865"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB13767.1"
                     /translation="MKTSRLKAFLFLLPALVFAQSTPTPLNQLAQGEAAEGWRLLFDG
                     KTMAGWDDPAKKSPPGDSWTIEDGCLKALANPKIGADLVSKETFGDFELAFDWRIAPA
                     GNSGVKYRIQERVFIDHGKRKPGAKRWEETVDYELLSRVSDRSRLAPGGRAEEYVIGF
                     EYQLIDDARNPDGAGSPTHRTGALYDMAAPAAGKSRPVGEFNSSRIVVRGNRTEHWLN
                     GAKVLDTLLDSPAVKESLARRWTPSSPVYRLLANPRTASCPISLQNHGDVAWFRNIRI
                     RRLDRR"
     gene            3128..5083
                     /gene="shc"
                     /locus_tag="VLE22_04870"
     CDS             3128..5083
                     /gene="shc"
                     /locus_tag="VLE22_04870"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HSB13768.1"
                     /translation="MGGFQLVEPAGSRRRAVSSATQQAEARASQQRRAEAARTSAEAL
                     LRLQEPEGYWRGDLTADISLEADYVLLQLWLHQPKGSGWNPPARARIEKCCSSILEWQ
                     LPDGGFALYPGGLSDVSLSVKAYTALKLAGWSPEAPEMVKIRETVLRLGGVDACNSYT
                     KINLSLFGLYPRRFVPTVPPEIALVPGGVLYEMSSWTRAIVVPLSIVQAAGRERRAPE
                     GFTLNELFLPGKRRSLVKGEGLATLFSHLDKLVKLWDKRGLKNLRALALREAEHWILD
                     RTRYSDGLGAIYPSMMYHVMALDSLGYPDDHPDLIEAIRQFDGLLTETPDRFFFQPCV
                     SPVWDTAIAAFVLGELGLAGQEELTRSADWLVSREVRRKGDWSVKRPNLEPSGWAFEF
                     ANEFYPDIDDTAMVLLAFCHARASAPEKQARTMRRAVNWLLGMQSSDGGWAAFDADNN
                     WRILNKLPFADHNAMLDPTCPDITGRVMEALVRTGVPRDDKAIRDGVKFLLGAQETDG
                     SWYGRWGVNYIYGTFLALRGLCASGSASAEVAMRKAAEWLQSIQNLDGGWGESCASYV
                     RNCYVPAPSTPSQTAWALLGLAAAGGTESLAVRRGLQYLISTQLPDGAWPEELATGTG
                     FPNVFFLRYHLYRHYFPLLALATCRES"
     gene            5087..>5574
                     /locus_tag="VLE22_04875"
     CDS             5087..>5574
                     /locus_tag="VLE22_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13769.1"
                     /translation="MKRREFVALLGAGRALCGRQGADWYKTPVIPTPEDPAQWPAFRE
                     ELKRWREEARQKLRYDGSLYSRPDFSWVTSSFACCFLMLCDSRFYDPKENRYLVEHWL
                     AEGERDFGGYDSVVLWHAYPRIGLDQRNQFDFYRDMPGGLEGVRDAARRFQNRGVRVY
                     ID"
BASE COUNT         1063 a         1709 c         1716 g         1086 t
ORIGIN      
        1 agtttccagg tgaggatgat acgcgttatg cgagagcctt tgttgaccta aacgctgatg
       61 ggaaagatga ggcgatcgtt ttcttgctcg gcagctcgtg gtgtggcagc ggaggctgcc
      121 caacccttgt tttgacgcct caagcggcct cttggagaag agtagcaagt attctcacca
      181 ccagtcctcc cattcgcgtg ttaacgagga gatccaacgg ctggcggaat attacagttt
      241 ggactcatga ccctggcgct ccaccctacg aagcagaact gcaattcgat ggtaagacct
      301 acccgctgag cccgacgatt ccagtaggga agaacgcggc tggaaaggtc atcatttcgt
      361 cgccgaaagc cgcttcgttt ctccgctgac cgatcctagg aactcaagga gtcagcccct
      421 tcaggcgctg actccgaccg ccgcgatctc tttgttcact tcggtcagga aggctttggc
      481 gtctccaaag agcatcaagg tcttgtccat gtagtaaagc tcgttgtcaa tgccggcgaa
      541 gcctgggttc aggctgcgtt tgatcaccat caccgtgcga gccttatcca catcgaggat
      601 cggcatgccg taaatcgggc tgctcttgtc ggttcgcgcc ccagggttgg taacgtcgtt
      661 ggcgccgatg acgagcgcca cgtccgcctg cgggaactcg gaattaatct cgtccatctc
      721 gaacagcttg tcgtaaggca cgtccgcttc ggcgagcaga acgttcatgt gtcctggcat
      781 gcgccccgca accgggtgga tggcgaaacg cacgtccacg ccccgtttcg tcagcagatc
      841 gaacatctct cgaagcttgt gctgcgcctg cgcgacggcc attccgtagc cgggcaccac
      901 gatgaccagg cgggcggcat cgaggatctg cgccgcttcc tccgccgtcg ccgaccgcgc
      961 ggtgcgctgc tcaacctcgt gggcctttac ctggagctgt ccgaaggcgc cgaagagcac
     1021 gttcgtgaac gaacggttca tcgcccggca catgatcacg gagagaatga agccggacga
     1081 gccgtcgagc gcaccggcga tcacgagcag gtaattgttc agagcgaagc ccatgaaact
     1141 cgccgacagg ccggcgtacg agttcaacaa cgcgatgacc gtcggcatgt ccgcgccgcc
     1201 gatcggaacg atcagcaata cgccgaaaat cagcgccagg atcgagaaaa tcggaaaggc
     1261 ccaagtcgct tcggggtgga tgcagaggta cacgccgaat aggaccgccg cgccgaggat
     1321 cgacaggtta aagatgttct gtcccttgta gacaatcggc cgctgtggca ggacctcctg
     1381 gagctttccg aacgccatca gacttccagt gaaggtgaga aagccgagca acacctcgat
     1441 ggacagcgcc accatcgtga agccatgggg cgtttcacgg tagtactcgg caacgccgat
     1501 cagggccgcc gccagcgcgc cgaaggcgtg cgagagcgct gtccgctgag gcacggcggt
     1561 catcggcatc agatatgcca gcgggacgcc gatggccgaa ccgatcagga aggcgatgaa
     1621 aatccactga tagttcacca cctcgaaccg caaaagcgtg ccgaccaccg cgaggagcat
     1681 gcccacctcg ccggcccata ctccgcgccg ggaggttgcg ggatggctca tccacttgag
     1741 tgcaaggatg aacagcgccg ccgcgataat atagctggcc tgaacgaaga gggtcgtcat
     1801 ttctttcccg gctcccgctt cttgaacatc ttgagcatgc ggtccgtgat caggtagccg
     1861 ctgaccacgt tgatcgtcgc cgccgtcacc gcgatgaagc cgagcacgcg ccccaggttg
     1921 gtggcgtgtg cctcgcctgt aatcaggatg gcgccgacca ccgagatcgc cgagatagcg
     1981 ttggtgagcg acatcagcgg cgtgtgcagc agcggtgaaa ccttcttgat cagctcgaga
     2041 ccgagaaagg tcgcgagcat gaacacatac acgcttaact catttaaagc cacagtcctg
     2101 gttcccctac agtggtttct ttggcaaatg cctctggaaa tgtcgcagtg tacaacatct
     2161 gccccctggt ttgcctgcca cccccgccag gcggctccct accgccggtc gagccgccgg
     2221 atcctgatgt tgcggaacca cgccacgtct ccgtggttct gaaggctgat cggacagcta
     2281 gcggtgcggg ggttcgccag cagccggtac accggcgatg agggcgtcca tcgcctcgcc
     2341 agcgactcct tgactgccgg agaatcgagg agtgtgtcga gcaccttcgc gccgttcagc
     2401 caatgttctg tgcggttacc acgcacgacg atacgggagc tgttgaattc gcccactgga
     2461 cgcgatttcc cagccgccgg tgcggccata tcgtagagcg ctcccgttcg gtgcgtcgga
     2521 gacccagcac catcgggatt ccgggcgtca tcgattagtt ggtattcgaa tccgattacg
     2581 tactcttcgg ccctgccgcc gggcgccaat ctgctccggt ccgacacccg gcttaacagt
     2641 tcgtagtcga cggtttcctc ccagcgcttc gccccgggct ttcgtttgcc gtgatcgatg
     2701 aagacacgct cctgaatgcg gtacttcacg ccgctgttac cggctggtgc gattcgccag
     2761 tcgaatgcca gctcaaaatc gccgaacgtc tccttcgaga ccagatctgc tccgatcttc
     2821 gggtttgcca gcgccttcag gcagccatcc tcgatggtcc aagagtcgcc tggtggactc
     2881 tttttcgccg gatcgtccca gcccgccatc gtcttcccat cgaatagcaa ccgccagcct
     2941 tcagcagcct cgccctgcgc aagctggttg agaggagtag gcgtggactg tgcgaacacg
     3001 agagcaggca gcaagaatag gaacgctttc agccgggagg ttttcatgtc tttgacaaca
     3061 gtccaggcga ttgtagcgcc acgcgcaccg ggtttggaaa tcagccgtct tctggcagca
     3121 tagaattatg ggcggctttc aattggttga gcccgccggc tctcggcgcc gagccgtttc
     3181 atcggcgaca cagcaagcag aagcgcgggc ctcacagcag cgccgcgccg aggcggctcg
     3241 aacatcggca gaggcattgc ttcgcctcca ggagccggag ggttactggc gcggggacct
     3301 gacggcggat atctcgctcg aagcagatta cgtcctgctt caactttggc tgcatcagcc
     3361 gaaaggttcg ggctggaatc cgcccgcccg cgccaggatt gagaagtgct gcagctcgat
     3421 tctggaatgg cagttaccgg atggcggctt cgcgctctat ccgggagggc tgtcggacgt
     3481 cagtctctca gtgaaagcgt acacggcgct gaagctggcc gggtggagcc cggaagctcc
     3541 cgaaatggtc aagatccggg agaccgtgtt gcggctggga ggcgtcgatg cgtgtaacag
     3601 ctacacgaag atcaatctca gcctcttcgg gctgtaccct cgacgcttcg tgccgaccgt
     3661 tccgccggag atagcactgg tgccgggcgg cgtcctttac gagatgtctt cgtggacccg
     3721 ggcgatcgtc gttccgctgt ccatcgttca ggctgcgggg cgcgagcggc gtgcgccgga
     3781 aggatttaca ctgaacgaac tgtttctgcc gggcaagcgg cgttcgctgg ttaagggcga
     3841 agggctcgcg acgctgttca gccacctcga caagctggtc aagctttggg ataagcgcgg
     3901 cctgaagaac ctccgcgcct tggcattgcg cgaggcggag cactggatcc tcgaccgcac
     3961 gcgttactcc gacgggctcg gcgccatcta cccgtcgatg atgtaccacg tcatggcgct
     4021 tgactcgtta ggctacccgg acgaccaccc cgacctgatc gaagcgatcc ggcagttcga
     4081 cggcctgctg acggaaaccc cggaccgatt cttcttccag ccgtgcgtct cgccggtatg
     4141 ggacacggcc atcgctgcgt ttgttctggg agaactcggc ttggccggcc aggaggaact
     4201 cacgcgcagc gcggactggc tcgtttcgag ggaagttcgc cgcaagggcg attggtcggt
     4261 gaagcgtcct aatctcgagc cgagcggctg ggcgttcgaa ttcgccaacg agttctatcc
     4321 ggacatcgac gacaccgcca tggtcctgct ggccttctgc catgccaggg cctccgcacc
     4381 ggagaagcaa gcccgcacaa tgcgccgggc ggtcaactgg ctgctaggca tgcaatcgag
     4441 cgatggcggc tgggccgcct tcgacgcgga taacaactgg cggatcctca acaagctccc
     4501 gttcgccgat cacaacgcga tgctcgatcc cacctgcccc gacatcactg gccgcgtgat
     4561 ggaagcgctt gtccgcaccg gggttccccg ggacgacaag gcgatccggg acggcgtgaa
     4621 gttcctgctt ggcgcccagg aaacggacgg gagttggtac ggccgatggg gtgtgaatta
     4681 catttacgga accttccttg cgttgcgcgg actgtgcgcc tcgggaagtg cgtcggcgga
     4741 agtggccatg cggaaggcgg cagagtggct ccagtcgatc caaaacttgg acggtgggtg
     4801 gggcgagagt tgcgcaagtt atgtccgcaa ctgttacgtc ccggcgccaa gcaccccgtc
     4861 gcaaacggca tgggcgctgc ttggcttagc cgcggccggt ggcacggagt ccctcgccgt
     4921 ccgccgtggc ctccagtacc tgatctcgac gcagttgccg gatggagcct ggccagagga
     4981 acttgccacc ggcaccgggt tcccgaacgt gttcttcctt cggtatcatc tctaccggca
     5041 ttacttcccg ctcctggcgc tagccacctg ccgggaaagc tagcgaatga aacgaagaga
     5101 attcgtcgca ctgctcggcg ccggccgcgc gctgtgcggc cgtcaggggg cagactggta
     5161 caagacgccg gtgattccca cccctgagga tcccgcgcag tggcccgcgt tccgcgagga
     5221 gctgaagcgc tggcgcgagg aagccagaca gaagctgcgc tatgacggct cgctttacag
     5281 tcgtcctgac ttctcgtggg tgacgagttc tttcgcctgc tgcttcctga tgctctgtga
     5341 cagtcggttt tacgatccca aagagaatcg ttacctggtg gaacattggc ttgcggaagg
     5401 cgagcgcgac ttcggcgggt acgattccgt ggtactctgg cacgcctacc cgcgcatcgg
     5461 tctggaccag cgcaaccagt tcgatttcta ccgcgacatg cctggaggat tggaaggagt
     5521 tcgcgatgcg gcgcgccgtt ttcaaaaccg cggcgtccgt gtctacatcg acta
//
LOCUS       DATIFQ010000115        13305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1760409, whole genome shotgun sequence.
ACCESSION   DATIFQ010000115 DATIFQ010000000
VERSION     DATIFQ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760409"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /gene="fusA"
                     /locus_tag="VLE22_04880"
     CDS             <1..1213
                     /gene="fusA"
                     /locus_tag="VLE22_04880"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSB13770.1"
                     /translation="TRSVSDSEPVSAFVFKTVADPFAGRLTYFKVCSGVLKNDANLTN
                     VRTGGAERLAHVGCLLGKSIQPVTELRAGDIGAVAKLKETLTGDTLADKASPISYPKV
                     KLPEPSIAFAVEAKTRQDEDRMGTAIQKILEEDQSLRFYRDPQTKEFLLGGSGQQHVE
                     VVVSRLKNRYGVDVTLKAPKVPYRETIRGTADVQGRHKKQTGGHGQFGDCWIRMEPLP
                     RGGNFEFVSEIFGGSIPRQYIPAVEKGIVEAAEKGYLAGYPVVDFRVAVYDGSYHDVD
                     SSELSFKLAGRKAFKAAMGVAKPALLEPIMNVEIQAPVEFAGDLMGDLNGRRGRISGM
                     ETRGNTQIIRAQVPMAEMLNYQNDLISMTQGRASFTMEFDHYDFVPQQQAEKIIAAAK
                     AAKEGVEEEEE"
     gene            1356..3047
                     /locus_tag="VLE22_04885"
     CDS             1356..3047
                     /locus_tag="VLE22_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSB13771.1"
                     /translation="MTDLRPYPYVFFSLLILAVLAPSTGCAPGRPQPFQASLLPPRPA
                     PPILADIPIEPPTVTPSLYLNELPAFLSEPREPELSSTSVDAILGRSLARYEAGKEHY
                     EAGEFAAAREDFDLAVEALLSAPNNLKDHQRIERKLEEIVEAIYRYDVAGLGAGELAA
                     EPAFDKSPLEEIPDATFPIDPRLANQVTEELLVTISQLPLDVNEEVLRYVKYFSSERG
                     RRIIANGLRRAGRYRSLIQRILDEEGVPQELIHLAQAESGFMPRAVSRKKATGMWQFM
                     QFRGREYGLVQTRSSDDRLDPEKATRAAARHLRDLYQKFGDWYLAMAAYNCGPGVIER
                     AVQRTGYADYWELKRRNVLPKETANYIPIIISMIIVSKNPADYGLDAVTPDPALEYST
                     LELDAPTHLALVADVLECPVSELLDLNPALLRNVAPEGYTLRVPKGKAGAVVSALEMV
                     PLEQRAAWRLHRVEPGETLATIAKRYRATAKGITDVNRLDGGAPEEGNLLLIPAAKQA
                     SKALAKPRVRAASAKRRATTRKASVRSGSRSSSSGKRSATIASKGSGTKRATLNR"
     gene            3153..3761
                     /locus_tag="VLE22_04890"
     CDS             3153..3761
                     /locus_tag="VLE22_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13772.1"
                     /translation="MVDDGRFALNGEDDEFTFDEEHIEMESEILSYGPDDEDEETELG
                     IEAEVQPEPAPKPPAPPVAPVPVAVVEPPAAPVPTKKPVSKAAKKAAAPKRVAVKAPA
                     KKAAKKAAPKKAAVKAPAKKAAKKAAPKKAAVKAAPKKAGKKVASKKAMPKKVAKKAA
                     PAMTARKAAVKAAPRKAAKKVASKKVAKKALRKPAKKAAKKK"
     gene            3832..4668
                     /locus_tag="VLE22_04895"
     CDS             3832..4668
                     /locus_tag="VLE22_04895"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HSB13773.1"
                     /translation="MPLNARRHIRKMRGGAQSHLIEADDGNYYVVKFLNNPQHRRILV
                     NELIAGVFAQYLQIATPETAIISVSPGFVAENPEVSIQLGSRSIPVSPGWHFGSRFPG
                     DPARVAVYDYLPDALLRQVANADHFLAVLVLDKWLSNADGRQSVYFRARIQDWDPRAA
                     VHPRQLGFVACMIDQGFVFNGPNWEFVDAPLLGLAPRLAVYERVCSLESFQPWLDQVV
                     GFPEHVVDKALSQVPAQWFDGDEEALERLLEQLMRRRARVPDLLLDCRRARIDLFPNW
                     NG"
     gene            4725..5597
                     /locus_tag="VLE22_04900"
     CDS             4725..5597
                     /locus_tag="VLE22_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HSB13774.1"
                     /translation="MHRFLLHNDQILDASSKVLSPGQVGLLSGWGVFSTIKVVSGVLF
                     AWERHWARMKRDSARMHIPFPGDPDTVLASLERLIEANGVRDATMRVAVLRNRGGMWE
                     GPGITRDYDLIAFTADLKGWGEGVNLAVMPQARHSACPFAGAKILSWAMNLAWLDEAQ
                     ARGFDEVILLNEHGEISECTSANIFIAEGNRVWTPPLSSGCLPGVTRELLLSEIHAPD
                     IVVGERTFKPSDLEKADEVMITSTTRDLLPVISVEGLQIRRNDRVRLKLQTAFSAFIE
                     GYVAVRTQAVPPRS"
     gene            complement(6812..7930)
                     /locus_tag="VLE22_04905"
     CDS             complement(6812..7930)
                     /locus_tag="VLE22_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13775.1"
                     /translation="MRRSLALAILCGASCLCAADRWELQFFHDAKDTTLAINDLKFSS
                     PGRGVAVGILTTKKGKTKPAMLVTSDGGRTWTYTSFKEIGISLFFLNESVGWLVTPDG
                     IWKTVEAGRSWTKLRKSPKDVYRVLFLDERSGWAVGADKGVWETKDGGQNWSRVAAAE
                     EVKSTRDYTFFSSITFVNGSTGIITGASVPPRRRGSPFPDWMDPELAEIRREWPAMNI
                     LLETRDGGKRWTSSTMSLFGRITRVVLAPQGTGLGLIEFFHFFEWPSEVFRLDWHSGK
                     SERVFREKNRAVTDIAITPDGEAYLAGFEPSGKLHHAPIPGKLKILRSSDLNQWQDMD
                     VDYRAVASRAVLSALDESHIWVATDTGMILRLTTGNAQ"
     gene            complement(7927..8535)
                     /locus_tag="VLE22_04910"
     CDS             complement(7927..8535)
                     /locus_tag="VLE22_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSB13776.1"
                     /translation="MPKVRDLELRLERIDQQLRLFQKISRMMVREMSLQEVLQGIVGL
                     VVEFTECDSCLVYLLDNDDLVLCASNTPHPSSIGKVRLKTSEGLTGWVARERRLLAIS
                     REAYKDPRFKAFGDLPEDSYEAFLSAPVIARNRVVGVINVQHRLPHPHTGGEMELLTT
                     VGEQVGCLLVLARMEPTAIEAANHVELVLSSGPVLPRPTGAA"
     gene            complement(8592..9551)
                     /locus_tag="VLE22_04915"
     CDS             complement(8592..9551)
                     /locus_tag="VLE22_04915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB13777.1"
                     /translation="MNVSRREFLGALAATTAVGAEGNALPTRVLGKTGQKVSILAFGC
                     GSRFLMYKDEDASVEALNRALDLGITYVDTAYGYGRGVSEQRVGKVMKTRRQGIFLAT
                     KVQDRKGDDAMRTFEGSLKRLQVDHVDLLHIHSLNDMEDLAAIEAPDGVLKTLYKIRD
                     QKMARFIGITCHSDPVALKTALERHDFNVTQMALNAALIGMANGARGMSPNPLGGPAS
                     FESVALPVANRKKMGVIGMKIFAQEKIVGQAPAQQLLRYVLSLPVTAAVVGMPKLEFL
                     DENIQTARTFTPMPKSEMQKLAGELADRNKVALDRYFSGHVDA"
     gene            complement(9659..10828)
                     /locus_tag="VLE22_04920"
     CDS             complement(9659..10828)
                     /locus_tag="VLE22_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB13778.1"
                     /translation="MRGSGDESRRFTHIQLKNWRNFLRVEATLERRTYLAGPSASGKT
                     NFLDLFRFLHDLVTPGVGFQEAVRKRGGLRKLRCLAARQDSDIGILVHAGAGGDPAGW
                     EYELHFNQESQPRPVIKRERFLSSGREVLVRPDERDSAAPECLERTLLEQESSRDDLR
                     ELAAFFRSIRYWHLAPQLVREPERPAVREQDAFGGDLLDRMAAMPEKTCRARLRFILE
                     TLHDAVPRIEQLDVRRDARGRPHLRARHEHWRSRGAVQTEQQLPDGALRLIGLLWTAL
                     EGGGPLLAEEPEISLHPQAVRLISGMLARLGRRSDRQLLMTTHSPDLVAGDSVRLSEI
                     LLFLPEDEATIVRPVLSAAEVRTMLEEGFGEREERPAGSAADDENQIELFDPSGA"
     gene            complement(10907..11326)
                     /locus_tag="VLE22_04925"
     CDS             complement(10907..11326)
                     /locus_tag="VLE22_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HSB13779.1"
                     /translation="MRQFLHTLAVSTAGKGLYEITRAVERWVSERGISEGLLTVFVRH
                     TSASLVIQENADPDVIQDLNSFFTRLVPEDDSLYRHTIEGSDDMPAHIRAALTGAQLS
                     IPVTGGRPALGAWQGIYVFEHRSIPHRRQVVLHLIGE"
     gene            complement(11323..13176)
                     /gene="glmS"
                     /locus_tag="VLE22_04930"
     CDS             complement(11323..13176)
                     /gene="glmS"
                     /locus_tag="VLE22_04930"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HSB13780.1"
                     /translation="MSGIVGYIGHRRAVPIILDGLRRLEYRGYDSAGIAVLDDDRRLE
                     VRRASGKLRNLEEAIRMAPVDGVYGIGHTRWATHGRPTAENAHPHRDCHGDVAVVHNG
                     IVENFLSLKQELLAGGHVFQTDTDTEVIAHLIEEHFDGSLEEAVRQSVRRLSGLFALA
                     IVSRSDPDRIVAVRSGPPVVIGLGAGEYFAASDVPAILSQTRDMFFLDDGEIAVLTPE
                     GVRLTDFDGRPVKHQVTHVLWDPIMAERGGYRDFTLKEIFEQPRAVRDTLAGRVEPDS
                     GKVILDELGIQPAELREFRQVRMVACGTSWHAALVGKFMIERLARLPVEVDYGSEFRY
                     RDPVLGPEVLTVIISQSGETADTIAALREAKQKGSKIVAVCNTPRSMVTREADGTLFT
                     HAGPEIGVASTKTFTAQLAALALLAMHLGQVRQVLDSAGSRDLAAELLRIPGKLEAVF
                     SSETFYEDLARGLYLARDFLFLGRGIQFPIALEGALKLKELSYIHAEGYPAGEMKHGP
                     NALIDQDVPVVVLAARDDSSDESRALYQKTLSTIQEVKARDGRVIAVAIEGDEEVRRI
                     LRDVIAVPPSIDLFLPMVEVVPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
BASE COUNT         2555 a         4055 c         4200 g         2495 t
ORIGIN      
        1 gactcgttcg gtctccgatt ccgagcctgt ttccgctttt gtcttcaaga cggtagctga
       61 tcccttcgcc ggccggctca cgtatttcaa ggtgtgctct ggcgtcctga agaacgacgc
      121 gaacctcacc aacgttcgca ccggtggcgc cgagcgcctc gctcatgttg gctgccttct
      181 cggcaagagc atccaaccgg tcaccgaact gcgggccggc gacatcggag cggtcgctaa
      241 gctgaaggaa acactcacgg gcgacactct ggccgacaag gcctcgccga tctcctatcc
      301 taaggtgaag ttaccggagc cgtcgatcgc cttcgcggtt gaagccaaga cccgccagga
      361 cgaggaccgg atgggtaccg ccatccagaa gatcctcgaa gaggaccagt cccttcgctt
      421 ctaccgcgat ccgcagacga aggagtttct gcttggcggc agcgggcagc aacatgtcga
      481 ggttgtcgtc agccgcctga agaaccgtta cggcgttgac gtcacgctca aagcgccgaa
      541 ggtgccgtac cgcgaaacca ttcgggggac ggccgatgtc caggggcgtc acaagaagca
      601 gaccgggggc cacgggcagt tcggcgactg ctggatacgg atggagccct tgccgcgcgg
      661 cggcaacttc gagttcgtca gtgagatatt tggcggctcg atcccccggc aatacatccc
      721 ggctgtcgaa aaaggcatcg tggaagcggc cgaaaaaggg tacctagctg gctatccggt
      781 ggtcgacttc cgtgtggcgg tctacgacgg ttcgtatcat gatgttgatt cgtcggaact
      841 ttccttcaag cttgccggcc gcaaggcctt taaggcggcg atgggggttg ccaagcccgc
      901 cctcctcgaa ccgatcatga atgtggaaat ccaggcgccg gtcgagttcg caggcgacct
      961 gatgggcgac ttgaacgggc gtcgcggccg catctccgga atggagacac gggggaatac
     1021 gcagatcatc cgggcccagg tccctatggc cgagatgttg aactaccaga acgacttgat
     1081 ctccatgacg cagggtcggg cttcgttcac catggagttc gatcattacg attttgtgcc
     1141 tcagcagcag gccgagaaga tcattgccgc cgccaaggcg gccaaagagg gcgtcgaaga
     1201 ggaagaggag taagagcccc tccccgtcgt cccggtggcg gcaatcccgc ggcaggactg
     1261 gggttgccct tgaccgccgt ccgtcgggtg ttatactgtc agagcgttgt aatcgaatgt
     1321 agagtccaga ctccagaaat acgccaggac tgcggatgac agatttacgt ccttatccat
     1381 acgtcttctt ctctttgctg attctggcgg tgctcgcgcc aagtaccggt tgcgctccgg
     1441 gaaggcccca gccgtttcag gcttccctgc tgccgccaag acccgcaccg cctatcctag
     1501 ccgacatccc catcgaaccc ccgacggtaa cccccagcct gtatctgaac gaactgccgg
     1561 ccttcttgag cgaaccgcgg gaaccggaat tgtcgtccac atcggtggac gcgatactgg
     1621 gccgttcctt ggcgcgctat gaggcgggca aggaacacta tgaggccggt gagtttgcgg
     1681 cggccaggga ggattttgac ctcgcggtcg aggcgctgct gtcagcgccc aataacctga
     1741 aggaccatca gcggatcgag cgaaaactcg aggagatcgt tgaggcgatc tatcgctacg
     1801 atgtggccgg gctgggcgcc ggcgaactgg cggcggaacc ggcgtttgac aagtcgccgc
     1861 tggaggagat ccctgacgcg acgttcccca ttgatccgcg tctggcgaac caagtcacgg
     1921 aagaactgct ggttacgatc tcgcaactcc ctctggatgt gaatgaagaa gtgctgcggt
     1981 acgttaagta cttctcgtcc gagcgtgggc gccggatcat cgcgaacggg ttgcggcggg
     2041 cgggccgcta tcgctccctg atccagagga ttctcgacga agagggcgtc ccacaggagc
     2101 tgattcacct ggcacaggcc gagtcggggt tcatgccgcg cgccgtttcc aggaagaagg
     2161 cgaccggcat gtggcagttc atgcagttcc gagggcgcga gtacggcctc gtgcagacgc
     2221 gcagcagcga tgaccggctg gatccggaaa aggcgacccg tgcggccgcc cggcacctgc
     2281 gcgacctcta ccagaaattc ggcgactggt acctggcgat ggccgcatac aactgcggcc
     2341 cgggcgtcat cgaacgggcg gtccagcgca cgggttatgc cgactactgg gaactcaaac
     2401 ggcgaaacgt actgccgaag gagacagcta actacattcc gatcatcatc tcgatgatca
     2461 tcgtgtccaa gaatcccgcc gactacggac tggacgccgt gacgccggat ccggctctcg
     2521 agtactcgac tttggagttg gatgcaccga cccacctggc gctggtcgcg gacgtcctcg
     2581 agtgcccggt cagcgagttg ctggacttga atcccgcact cctccggaac gtcgcgccgg
     2641 aaggctacac attgcgcgta ccgaaaggga aggctggggc agtcgtctcg gctcttgaga
     2701 tggtgccgct cgagcagcgg gctgcatggc ggcttcaccg ggtggagccc ggtgaaaccc
     2761 tcgccacaat cgccaagcgg taccgggcca ccgccaaggg catcacagac gtgaaccggc
     2821 tcgacggcgg cgcccccgag gaaggcaacc tgctcctgat ccccgccgcc aagcaggcgt
     2881 ccaaggcgct ggcgaagcca cgcgtacgcg ccgcgtccgc caaacggcgt gccacgacgc
     2941 gaaaggcttc cgttcggagc ggatctcggt catcatcctc gggcaaacga tctgcgacga
     3001 tcgcatcgaa ggggtccgga acgaagcggg ccaccctcaa tcgctgatcg gcgcagggct
     3061 gtgataacac cggcttcgtg cttttcccct tgccttgatc ggaaaacgag gtattctatc
     3121 tgtgaaccat gcccaaacag gaggcactga gaatggttga cgatggcaga ttcgcgctca
     3181 acggcgaaga tgacgagttc acgttcgacg aggagcatat cgaaatggag tctgagattc
     3241 tgtcgtatgg accggacgac gaggacgagg agacggaact cggtatagag gccgaagtgc
     3301 agccggaacc ggccccgaag ccccctgcac cacccgtggc accggtgccg gtagcagtgg
     3361 ttgagccgcc agcggctccg gtgcccacga agaagccggt gagcaaggcg gcaaagaagg
     3421 ccgcagctcc caaaagggtg gcggtgaagg cgccggccaa gaaggccgca aagaaggccg
     3481 ctcccaagaa ggcggcggtg aaggcgccgg ccaagaaggc cgcaaagaag gccgctccca
     3541 aaaaggcggc ggtgaaggct gctccgaaga aggcagggaa gaaagtggca tccaagaaag
     3601 cgatgcccaa gaaagtggcc aagaaggccg ccccagccat gaccgcacga aaagcggccg
     3661 taaaggcggc cccgaggaaa gcggcgaaga aagtagcgtc caaaaaggtc gccaagaaag
     3721 ctcttaggaa gcccgcgaag aaggcggcga agaagaagta aggcactgcc ccacttttac
     3781 tccacttggg acaccacgga tgggccgtca gccgggtaga ctggcatagc gatgccgttg
     3841 aatgcccggc ggcacatccg gaaaatgcgc ggcggagcgc agtcgcactt gatcgaggcc
     3901 gacgacggaa actactacgt cgtcaagttt cttaataacc cacagcatcg gcgcatccta
     3961 gtcaatgaac tgatcgccgg tgtcttcgct caatatcttc agatcgccac cccggagacg
     4021 gcgatcatct ccgtaagccc tggattcgtt gcagagaacc cggaagtatc gattcaactc
     4081 ggctcgcgga gtatcccggt gagtccagga tggcacttcg ggtcgcggtt tccgggtgac
     4141 ccggcgcggg tagccgtgta tgactacctt cccgacgcgc tgctgcggca ggtcgccaat
     4201 gcggaccact tcctcgccgt actggtcctg gacaagtggc tttcaaacgc cgatggccgc
     4261 cagtcggtct actttcgggc gcggattcag gactgggatc cgcgggcggc ggtgcacccc
     4321 cggcaactcg gttttgtcgc ttgcatgatc gaccagggct ttgtattcaa cgggccgaac
     4381 tgggagttcg tcgatgcacc gctgctgggg cttgcgccga gacttgctgt gtatgagcgg
     4441 gtctgctccc tggagagctt tcagccctgg ctcgaccagg tggtcggctt tccggagcac
     4501 gtcgtcgaca aggcgctgag tcaggtcccg gcccagtggt ttgacgggga tgaagaggcc
     4561 ctggaaaggt tgctcgagca gttgatgagg cgtcgagcgc gcgtgcccga cttgcttctg
     4621 gactgccggc gggcgagaat cgacctattc ccgaactgga acggctgacg cattcgacag
     4681 gtccggtcgc ggcagtcgaa aaatcctaat agaatagtga cttcatgcat cgatttctcc
     4741 ttcacaacga ccagatcctc gacgcatcga gcaaagtgct ttcgcccggc caggttggcc
     4801 tgctttcggg ttggggagtg ttctcgacaa tcaaggtcgt cagcggcgtc ttgtttgcct
     4861 gggagcgcca ctgggcgcga atgaagcggg attcggcgcg gatgcacatc ccgtttcccg
     4921 gcgatccgga tactgtgctg gcgagcctcg agagactcat cgaggcaaac ggcgttcggg
     4981 atgcgacaat gcgggtcgca gtattacgga accgcggcgg catgtgggaa ggaccgggaa
     5041 tcactcgcga ctacgacctt attgcgttca ccgcggacct caagggttgg ggtgaaggag
     5101 tcaacctggc tgtcatgccg caagcgcggc actccgcgtg tccgttcgcg ggcgcgaaga
     5161 tcttatcctg ggcgatgaac ctggcatggc tcgatgaagc tcaggcccgg ggctttgatg
     5221 aggtcatcct gctgaacgag cacggggaga tcagcgagtg cacgtccgcc aacatcttca
     5281 tcgccgaggg aaaccgagtg tggactccgc cgctcagctc cggttgcctt ccaggcgtca
     5341 cccgtgagct tctcctgtcg gagattcacg cgccggacat cgtggtaggg gagcgcacat
     5401 tcaagccctc cgacctggag aaggccgacg aggtaatgat tacgtcgacc actcgagatt
     5461 tgctgcccgt aatctcggtc gagggacttc agatccggcg caatgacagg gtccggctga
     5521 agcttcaaac ggctttcagc gcctttattg aaggctacgt cgccgtccgc acgcaggcag
     5581 ttccaccaag gagttgaccg cggcccgccc tcggcctgcc gggattctga gcgagcgatg
     5641 gatattcgat gtccgcagtg tggaagccgt gacctccgtt gttctcatct gcgtaccccc
     5701 ggggagcgga tcgcaagctt gatgggcgcg cgcccagtcc gttgccgcga gtgccgtgcg
     5761 cgtttctctt ggcgcctttg gaagttctcg gatctccgct atgcgcgctg cccgaagtgc
     5821 ctgcggatgg acttggggat ctggtctgag ggccactatc gattgccaac cttccggagt
     5881 atccttctgc agctcggggc gaatccatac cgctgcgaat actgccgcta caacttcgtg
     5941 agtttccggc ggcgtaagga gcgcttcagt ttccatagat ggaaagagaa gagggaggtg
     6001 caagtcaaga gtgccgccag ccgaagacgg gaaggggagg acgctcaaga aacagaaagt
     6061 ggttccaagt gacccacttt ccagcttgct ctccaaccgg catgcccggc ccgagcgtag
     6121 cacacagtca aaccgaacgc tttctccgtt gctccacgtg accggttttg gccgaccacg
     6181 cttcgatgct ccgaagagcg cctgcaagtc tgactgctag aagccgacgt ctgagaacgg
     6241 cctttcactc gcccacagcg attgctcgtt ttacggagcc gccacggcag ggtcgtcgat
     6301 cccgacctat tctttcaacg tcccaccgga ccgttgatga acccgttcgg cctttggact
     6361 cccttacccg ccaccgattg ccgccggtca cggtgaagat cactgccctc ggcccgttgt
     6421 ccacctcgga tccagctgtc ccgacctctc ctcgcgcttc cactcccctt tcgggttttc
     6481 aggcccgttc ggatcgcagt gctcggcgcg attcctgatc aggaaactta cccctccgga
     6541 ctgcccgatt gcccttcgct cccctccggc gttgtttctt agtcaatccc gccgaaggct
     6601 caccgttcca ggctcgctac aacccgtgag gcccgctgtt ccgtgaacct cttggaacca
     6661 tccccataat gcactcgatc caaatccaag tcaaacgaaa tcgtcatcgg atcttagctt
     6721 ttccagagat tttattgcct ttataatcaa cacgataatg acacttccgg tcggatttgt
     6781 tgtgaataaa acaggatggc ggtcaagtgt tttattgagc gttgcccgtt gtgagccgca
     6841 gaatcattcc ggtatccgta gcaacccaga tgtggctttc gtctaacgcc gagagtacag
     6901 cacggctcgc cactgcacga tagtccacgt ccatgtcctg ccactggttg aggtcgctgc
     6961 tgcgaaggat cttgagcttg cccggtatgg gggcgtgatg gagtttcccg ctcggttcaa
     7021 atccagcgag ataggcttcg ccatcgggcg tgatcgcaat gtccgtcacg gctcgattct
     7081 tctcacgaaa cacgcgctcg cttttcccgc tgtgccagtc gagacggaag acttcggaag
     7141 gccactcgaa gaaatggaag aactcgatca acccgaggcc cgtgccctga ggggcaagga
     7201 caacccgcgt aatgcggccg aagagcgaca tggtggagga ggtccaccgc ttgccgccgt
     7261 cccgagtttc gaggaggatg ttcatcgccg gccattcgcg ccgtatttcc gccagctccg
     7321 gatccatcca gtctggaaac ggggagcccc gccggcgcgg cggcacgctc gctccggtaa
     7381 tgatgcccgt actcccgttt acgaacgtga tcgagctgaa gaacgtgtag tcccgcgtgc
     7441 tcttgacttc ctcggcggcg gcgacccgac tccagttctg gccgccgtcc tttgtctccc
     7501 agactccctt gtcggctccg acggcccagc cgctccgctc gtcgagaaaa agaacgcggt
     7561 agacgtcctt tggactcttc cgcaatttcg tccaactccg gcccgcctcc actgtcttcc
     7621 agattccgtc cggcgtaacc aaccacccca cggactcatt cagaaagaac agggagatcc
     7681 cgatctcctt aaacgaagtg taggtccagg tgcgcccgcc gtcgctggtg accagcatcg
     7741 caggcttggt cttgcctttc ttcgtggtca gtatcccaac ggcaacaccg cggccggggg
     7801 aggagaactt gagatcgttg atcgccagag tggtgtcttt ggcgtcgtga aagaactgaa
     7861 gctcccagcg atcagccgcg caaagacagg acgcaccgca gaggatggcg agcgcaagag
     7921 atctcctcat gcggcgcccg tcggccgggg aaggacgggc cccgaggaga ggaccagttc
     7981 gacgtggttg gcggcctcga tcgcggtcgg ctccatgcgc gcgagcacca gcaggcagcc
     8041 gacctgctcg ccgacagtgg tcaggagttc catttcaccg cccgtgtgcg ggtgcggcag
     8101 ccggtgctgc acgttgatga cgccgacgac gcggttgcgg gcgatgacgg gagcggataa
     8161 aaaggcctcg tagctatcct cgggcaagtc gccgaacgct ttgaagcgcg ggtctttgta
     8221 ggcttcgcgg gagatggcga gcagccttcg ttcgcgggcc acccatccag tcaaaccttc
     8281 cgaggtcttc aggcgcacct tgccgatact cgagggatgc ggcgtgtttg aagcgcagag
     8341 gaccaagtcg tcgttatcaa gcaggtagac aaggcaggag tcgcactcgg tgaattcgac
     8401 aacgagaccc acgatgccct gcaggacctc ctggaggctc atctcgcgca ccatcatccg
     8461 gctgatcttc tgaaacagcc gcaactgctg gtcgatgcgc tcaaggcgca actccaagtc
     8521 ccggaccttc ggcactcccc tattatccca aacgctcagg tgtctgaatc gggcgctgtg
     8581 gacagcctgg attatgcgtc gacgtggccg gagaagtaac ggtcgagtgc gactttgttg
     8641 cgatcggcga gctcgcccgc aagtttctgc atctccgatt ttggcatcgg cgtaaatgtc
     8701 cgggcggtct ggatgttctc atcgaggaac tcgagcttgg gcatgccgac tacggcggcg
     8761 gtgaccggca aagagagcac gtaccggagc aactgttggg ctggcgcctg gcccacgatc
     8821 ttctcctgag cgaagatttt catgccgatt acacccatct tcttcctgtt ggcgacaggc
     8881 agtgcaaccg actcgaagct ggcgggccct ccgagcggat tgggggacat cccacgcgcc
     8941 ccgtttgcca tgccgatgag agcggcgttg agcgccatct gcgtgacgtt gaagtcgtgc
     9001 cgttcgagcg cggtcttcag cgcgaccgga tcgctgtggc aggtgatgcc gatgaagcgc
     9061 gccattttct ggtcgcggat cttgtaaagg gttttgagaa cgccgtcagg agcttcgatg
     9121 gcggcgaggt cctccatgtc gttcaggctg tggatgtgga gcagatccac atgatcgacc
     9181 tggagccgtt tcaggctgcc ctcgaaggtc cgcatggcgt cgtcgccctt gcggtcctgg
     9241 actttcgtcg cgaggaagat tccctggcgg cgggtcttca tcaccttgcc cacccgctgt
     9301 tcgctgacgc cgcggccata gccgtacgcg gtgtcgacgt aggtgattcc cagatccagc
     9361 gcgcggttca acgcttcgac ggaagcatcc tcgtccttgt acatcaggaa gcggctgcca
     9421 cagccgaagg cgaggattga gactttctgc cccgtcttgc ccagaactcg cgtgggaagt
     9481 gcattgccct cggcgccaac ggccgttgtc gccgccaatg cacccagaaa ctcacgtctt
     9541 gaaacgttca ttatccgctc cttacaccca gagggtcccg atttttatga caggtcccac
     9601 ctcatcggtg ttcaccagcg tatcctacta cgcggcgcgc cttcaactgc tcgcaggctc
     9661 atgccccgga ggggtcgaac agctcgatct gattttcgtc gtccgccgca ctaccggctg
     9721 gacgctcctc gcgctcgccg aagccttcct caagcatggt ccggacctcg gccgcactga
     9781 gaaccggccg gacgatcgtt gcctcatcct ccgggaggaa cagcaggatc tcggaaagcc
     9841 gcacggaatc accggccacg agatcgggcg agtgggtggt catcagcagt tgccggtccg
     9901 agcggcgccc gagacgggcg agcatgcccg atatcaggcg gaccgcctga gggtggaggg
     9961 agatttccgg ctcctccgcc agtagcggcc cgccaccttc gagcgctgtc cacaagaggc
    10021 cgatcaaccg aagcgcgcca tcggggagct gctgttcggt ctggactgcc ccgcgcgagc
    10081 gccagtgctc atgcctggcc cgcagatggg gccggccgcg ggcgtcgcgg cgaacgtcga
    10141 gctgctcaat ccgcggcacg gcgtcgtgga gcgtttcgag gatgaacctc agccttgcgc
    10201 ggcatgtttt ttcgggcatc gcggccatac gatcgaggag gtcgccacca aacgcgtcct
    10261 gctcgcggac agccggccgt tccggttccc ggacaagctg cggggcgagg tgccagtagc
    10321 ggatcgaccg gaagaaggcg gctagctcgc ggagatcgtc ccggctggat tcctgctcta
    10381 gcagggttcg ttccaggcac tcgggcgcgg cggagtcgcg ctcatcgggc cggacgagca
    10441 cctcgcgacc gctgctcaga aaacgctccc gcttgatgac tggccgcggc tggctctcct
    10501 gattgaaatg aagctcgtat tcccagccgg ccgggtcgcc tccggctccc gcgtggacga
    10561 gaatgccaat gtcggagtcc tggcgggcgg cgaggcagcg cagtttgcgc agtccgccac
    10621 gcttccgcac ggcttcctgg aatcccacgc cgggtgtcac gaggtcgtgg agaaagcgga
    10681 agaggtcgag gaaatttgtc tttccggaag cgctgggccc ggcaaggtaa gtgcgccgct
    10741 caagggtggc ttcaactcgc agaaagttcc gccagttctt gagctggatg tgggtgaagc
    10801 gccggctctc gtcgcccgat cctcgcatgc gcgctcagcg cgtcctttcg ggagtaccca
    10861 gtgtgacacc gcggcacgga ggacgcaaga gccgcgaagg cccgccctac tcaccgatga
    10921 gatggagaac gacctggcgg cggtgcggga tggagcggtg ctcgaacacg tagattccct
    10981 gccaggcgcc gagcgcgggg cgtccaccgg tgacgggaat ggaaagctgg gcgccggtga
    11041 gcgcggcgcg gatgtgcgcg ggcatgtcgt ccgatccttc gatggtgtga cggtacaggc
    11101 tgtcgtcttc gggtaccagg cgggtgaaga aggagttgag gtcctggatc acgtcggggt
    11161 cggcgttctc ctggatcacc agagacgcgg aagtgtgccg gacaaaaaca gtcagcaggc
    11221 cttccgagat gccacgctcg gacacccatc gctccaccgc tctggtgatc tcgtagagcc
    11281 ccttgccggc tgtggatact gccagagtgt gaaggaattg cctcattcga cagtgacgct
    11341 cttggcgaga ttgcgcggct ggtcaacgtc acagccgcga cgcacggcga tgtggtaggc
    11401 gagaagctgg agcggcacga cctccaccat cggcaggaag aggtcaatcg aaggcgggac
    11461 ggcgatgacg tcgcgtaaga tccgccgaac ctcctcgtcg ccttcgatgg ccacggcgat
    11521 gacgcgtccg tcgcgggcct tgacctcttg aatagtggac aaggtcttct gatagagggc
    11581 gcggctttcg tcgctcgagt cgtccctcgc cgccagcacg acgaccggga catcctggtc
    11641 gatcagcgcg ttgggaccgt gtttcatctc gccggccggg tagccttcgg cgtgaatata
    11701 ggaaagctct tttagcttga gcgcaccctc gagagcgatc ggaaactgga tgccgcggcc
    11761 gagaaacagg aagtccctgg cgaggtagag tcctcgggct aaatcctcat agaacgtctc
    11821 gctggagaaa accgcctcga gtttgccggg aattcgcagc agttcggcgg cgagatcgcg
    11881 cgaccccgcg gagtcgagca cctgccggac ctggccaagg tgcatagcca gaagcgcgag
    11941 ggccgcgagc tgcgcggtaa aagttttcgt cgatgcgacg ccgatttcgg gtccggcgtg
    12001 cgtgaagagc gtgccgtcgg cttcgcgcgt caccatcgag cgaggtgtgt tgcagacggc
    12061 gacgatcttc gatcctttct gcttggcttc gcgaagcgct gcgatcgtgt cggcggtttc
    12121 gccggactgc gagatgatca ccgtgagaac ttcggggccg agaaccgggt cgcgatatcg
    12181 gaactcgctg ccatagtcca cctcgacggg aaggcgcgcc agcctttcga tcatgaattt
    12241 gccgaccaaa gcggcgtgcc agcttgtgcc gcaagcgacc atcctgactt gacgaaattc
    12301 ccgaagttcc gccggctgga tgccgagttc gtcgagaatg accttgccgg agtccggctc
    12361 gacacggccc gccagcgtgt cgcggaccgc gcgcggctgt tcgaatatct ccttcagcgt
    12421 gaagtcccgg tagccgcccc tctcggccat aatcggatcc cacaagacgt gtgtaacctg
    12481 gtgcttcacc ggacggccgt cgaagtcagt caggcggaca ccctcaggcg tcagcacggc
    12541 gatctcgcca tcgtccagga aaaacatgtc ccgagtctgg ctcaggattg cgggcacgtc
    12601 cgaggcggcg aaatactcac cagcgccaag tccgatgact acgggaggcc cggagcgcac
    12661 ggcgacgatg cggtccggat ccgagcgcga gacgatagcc agcgcaaaca ggccggacag
    12721 ccgacgcact gattgccgca cggcctcctc gagactaccg tcgaagtgct cttcgatgag
    12781 gtgcgcgatg acctccgtgt cggtgtcggt ctggaaaacg tgaccgccgg cgaggagttc
    12841 ctgcttcagg gagagaaagt tttcgacgat gccgttgtgc accacggcga catcgccgtg
    12901 acagtcgcgg tgcgggtggg cgttttccgc ggtcggacgg ccgtgcgtgg cccagcgtgt
    12961 gtggccgatc ccatacactc cgtcgacggg ggccattcga atggcctcct cgagatttcg
    13021 gagtttgccg gaagcgcggc gcacctccaa ccggcggtcg tcgtcgagca cggcgatgcc
    13081 cgccgagtcg taaccgcggt attccaggcg gcgcaggcca tccagaatga tcgggaccgc
    13141 cctgcgatgg ccaatgtagc caacgattcc ggacatcggg cgggctgcct agtcgaggat
    13201 ctcaacccgg tattcttcaa tgaccggatt ggccagcaca tcgtgagcga tctgctcgat
    13261 ctccttccgg acctcgttgg gaccggactc gggagcgaga gtgat
//
LOCUS       DATIFQ010000116         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_420803, whole genome shotgun sequence.
ACCESSION   DATIFQ010000116 DATIFQ010000000
VERSION     DATIFQ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420803"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VLE22_04935"
     CDS             <1..1375
                     /locus_tag="VLE22_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB13781.1"
                     /translation="GRLHMNELTRRDFARSAGFASALSYSRILGANDRVRLGYIGLGN
                     RGDQVHEAFLEHGDCQTVAVCDLRDDYMDLAVKKSRATPRKYKEYKKLLDDRDVDAVV
                     IATPDHWHALMFIDACNAGKDVYVEKPLSLTVHEGRKMVETAERTKRVVQVGTDRRSW
                     KTYKEAVDFVRSGGIGHVSVARCFHIRNEWPNGLGPAPDGPPPSEWEWDQWLGPAPKV
                     PYNRNRTYYNFRWFYDYSGGQLTNFGVHYVDVIRWLLGQDSPRAVTALGGQYAGIRDN
                     REIPDTLEVLWSFDGPTLVVFSQFNANAAPGNVKDSEIELRGTKGTMYIRAMSWEVVP
                     ERTTEAYIGYDQGKGYGNPVDRTASRRYSASLKPAMEPIPLRGSGGYDTIPHARNFLD
                     CIKSRGKCNADILTGHLSTSATLIGNIALKTESHLKWDARAERFTNKPEANRFLQYQY
                     RTPYKLI"
     gene            complement(1419..>2280)
                     /locus_tag="VLE22_04940"
     CDS             complement(1419..>2280)
                     /locus_tag="VLE22_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13782.1"
                     /translation="YAVRCSTGRWPLVIGEKQGAVVVASECGGFANLGFRRVRDLEPG
                     EIVLLKDGVWETKGRISGGTVRVCSFYWVYTGFPTNVFEGVPDSLVRKRLGAALARRD
                     IENEFIPDVVIPVPDSGRFHAIGYQQEFCRQMMAGKVQNVPVYDELLVKYPYAGRSFI
                     PQDQKQRDREARVKLLASGEDCSGKVVAVCDDSIVRGTQAQTDLVPKLRQTGVREIHF
                     RISNPELRSYCPWGKTTRKGELLADRVPAIADRVKYLGVDGLKYNTIQDLSEAIGRPL
                     ESLCVDCALG"
BASE COUNT          476 a          692 c          670 g          442 t
ORIGIN      
        1 tggaaggctc catatgaacg aactcactcg aagggatttc gctcgaagcg ccgggttcgc
       61 ctcggcgctc agctactctc gcattctcgg cgccaatgat cgtgtgcgcc tgggttatat
      121 aggtctcggc aaccgtggtg atcaggtcca tgaggcgttt ctcgagcacg gggactgcca
      181 gacagtagcg gtgtgtgatc tccgcgatga ctacatggat ctggctgtga agaagtcgcg
      241 ggcgactccg agaaagtaca aggaatacaa gaagcttctc gatgacaggg atgtggatgc
      301 ggtggtcatc gccactccgg accactggca cgcgctgatg ttcatcgatg cctgcaatgc
      361 aggcaaggac gtttacgtcg agaagccgtt gagcctgacc gtccacgagg ggcgaaagat
      421 ggtggagacc gcggaacgca ccaaacgggt cgttcaggtc ggcactgacc gtcgctcctg
      481 gaagacgtac aaggaggcag tggacttcgt gcgctcaggc ggcatcggcc acgtgtcggt
      541 agctcgttgc ttccatatcc gcaacgaatg gccgaacggc ctgggtccgg cccctgacgg
      601 tcctccgccc agcgaatggg aatgggatca gtggttgggc ccggcgccga aggtgccgta
      661 caaccgaaac cgcacctact acaatttccg ctggttctat gactattccg gcggccagct
      721 tacgaacttc ggcgttcact acgtggatgt gattcgatgg ctgctggggc aggactcgcc
      781 acgcgcagtc accgctctcg gcgggcagta cgccggcatc cgggacaatc gggagatccc
      841 cgacacgctg gaagtcctgt ggagtttcga cggtcccacg ctggtggttt tctctcaatt
      901 caacgccaat gcggcgcccg gcaatgtgaa agactccgag atcgagcttc gcggcaccaa
      961 aggcacgatg tacattcggg ccatgagctg ggaagttgtg ccggaacgga ccaccgaggc
     1021 atacattgga tacgatcagg gcaagggata cggcaaccca gtggaccgta ccgcctcccg
     1081 ccgatattcc gcttccctga aaccggcgat ggagccgatc ccgctgcggg gttcaggcgg
     1141 ttacgatacg attccacacg caagaaactt tcttgactgc atcaagagca ggggcaagtg
     1201 caacgccgat atcctgacgg gacatctctc gacgtctgcc actttgatcg gcaacatcgc
     1261 tctcaaaacc gagagccatc tcaagtggga cgccagggcc gagcgattca ccaacaaacc
     1321 ggaagccaac aggttcctgc aataccagta ccgaacgcct tacaagttga tctgagatgt
     1381 gagacgcgga ccttggagcg cctgccgttt ctagcgcctc agcccagggc gcagtccacg
     1441 cagaggcttt ccaacgggcg tccgatggct tcggagaggt cctgaatcgt gttgtacttg
     1501 aggccgtcga cgcccaagta cttcactctg tcggcgatcg ctggaacccg atctgcaagg
     1561 agttcaccct ttctcgtcgt cttgccccag gggcaatagg agcgtagctc cgggttagag
     1621 atgcggaagt ggatttcacg cacgccagtc tgtctcagct ttggcaccag gtcggtttgc
     1681 gcttgtgtcc cgcgcacgat cgagtcgtca cagacggcga cgaccttgcc gctgcagtcc
     1741 tctccgctcg ccagcagctt cacacgcgcc tcgcggtccc tctgcttctg gtcctgcggg
     1801 atgaaactcc tgccggcata ggggtatttc accagcagtt cgtcgtagac gggtacgttc
     1861 tggacctttc cggccatcat ctgccggcag aattcctgct gataaccgat cgcatgaaac
     1921 cttcccgagt ccggcacagg gatgacaaca tccgggatga attcattctc gatgtcacgg
     1981 cgcgccaaag ccgctccaag acgcttccgg accagcgagt cggggacacc ctcaaacacg
     2041 ttggtgggga aaccggtgta gacccagtag aagctgcaaa cccgaaccgt tccgccggag
     2101 atcctaccct tcgtttccca tacaccatct ttcaacagga cgatctcgcc gggctcgaga
     2161 tcccgaaccc gccggaagcc gaggttggca aaaccgccgc attcggacgc aaccacgacg
     2221 gctccctgtt tctctccaat gaccagcggc cagcggccgg ttgagcaccg gaccgcatag
//
LOCUS       DATIFQ010000117        27276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2719731, whole genome shotgun sequence.
ACCESSION   DATIFQ010000117 DATIFQ010000000
VERSION     DATIFQ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 27276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27276
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719731"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VLE22_04945"
     CDS             <1..517
                     /locus_tag="VLE22_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13783.1"
                     /translation="SPNPGSSEENYRDNPTPDTKGKVVVSDTDHLWGHTGGDAVWVWK
                     SFTRGLNVLLMEEFTPSPAWQDSAREAMGQARRYAEKMDLAAMTPAAELCSTGYCLAA
                     KGREYLAFMPGSPGQFNVNLTDVAGGCSAEWLNVTTGKTVPGQEVRGGARRTFATPFG
                     GPAVLYLKCGG"
     gene            complement(561..1604)
                     /locus_tag="VLE22_04950"
     CDS             complement(561..1604)
                     /locus_tag="VLE22_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB13784.1"
                     /translation="MSIPVSVVFNPNWWFRNYDVSFDRPFYFHRETIVRNDVVMRRAL
                     YDRFGLGEADPQPRPIAGSRHVAGGFVVPALLGVEIRFSDNQAPWNVPRNLSREEALR
                     LRVPEIESTWPMNEWSALMDGLEKECGRLTGDFNTGGLINTALELRGNDFFLDLLEDP
                     ETTDHVFTVIAETQLRVANYVRSRTGTSSVSVNRGILHVDPGLYLHASCSVQMISPSL
                     YERAILPHEQLLAERLTPYGIHHCGNNLHLFAQSYSRLPVRFLDVGWGSDVAACARVF
                     PEAFLNLRLSPVRMLQESSETIRRDTIELLRAAGRTANVGVCCINMDYGTPDANVLAM
                     FRAAAEFPGNARK"
     gene            1791..2324
                     /locus_tag="VLE22_04955"
     CDS             1791..2324
                     /locus_tag="VLE22_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13785.1"
                     /translation="MKRLVWITLWLLAPIFVPAATLERLSLDEMIEKSTAIVRGKIVS
                     AGASFHGPVIYTHFSVQVLEHWKGDDVAQVDVVVPGGTAKGLHQTFAGTPQLEPGGEY
                     VLFLWKGKSGLTHVIGLSQGVFTLKSDGDGGMLATRSASTEAMLDRRTGQVVNDETLR
                     LRVGDLRARVSAAARAR"
     gene            2336..4876
                     /locus_tag="VLE22_04960"
     CDS             2336..4876
                     /locus_tag="VLE22_04960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HSB13786.1"
                     /translation="MVAARKLMVAAVAATLLSGTAFAHYHFLHYKSRNAPFAPVPEKF
                     DLKALPNQTLFYFISDQGPEQMAPGDSRASFLSQIRMAADVWSGVETSELRLEFGGLF
                     TPGALQANPGVDILFDEIPPGLVAMGGPTVRASMVDAEANSFVPILRSVLILQKDLSQ
                     RPSYTDSFFQTVVHEMGHALGLQHTMTSSVMATQVTRATTKSRPLAADDVAGLSALYP
                     AQAFAERTGSVSGRVATDGVGVHLASVVAVAPNGTAVSTLTDPDGHYRLVGLTPGQYY
                     LYVHPLPPAAQQGLGPADVVLPVDPDGQPFEAGPVFETVFYPGTKNIQAAAVLGVQAG
                     VTLEGYDFPVTRRGGLDLFGVTTYSFPGNYAVKPAFINVNGPRNFLVASGVGLTTDSA
                     PSPGLTAAVMGGTALIPEGGIKAYSPAPVFLQLDFGFNPFSGEGPRHLLFSRNNDLYV
                     LPAGLNLVNKQPPSITAVSPALDGSGNRVAILSGSNLTRDTRILFDGLRAQVVSADDT
                     AGTLTVVPPVGGPNHQAVVTALNGDGQTSMFLDAAAPPQYKYDAVEAAPYLSVLPSSL
                     PAGSEAMVDIYGANTHFTPGQTVIGFGTSDIVVRRMWVLSPDRLRANVHVGTLAAEGI
                     ARLTVVTGFEIVEHPFALLVQPANPAAFVVNPRAVNPMTGLPSIYAGGQAAVEVANLP
                     AGAAVAVTLNDQPATVVSVEDGRLIFVVPAGLGTGTAILRISVGTVAAYPVVLVLDAA
                     PPAVLAISKSPVVPGEELVVTVAGLGDPGVIGSPRKVRILVGGVEHAAAEVTPSESDP
                     AVYLVRFVLSPAVPQGDQVPLIVRIGDRASLPVPIAVHSPEQPPTDSL"
     gene            4918..5199
                     /locus_tag="VLE22_04965"
     CDS             4918..5199
                     /locus_tag="VLE22_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13787.1"
                     /translation="MAFEADKTMFEIYRETAYSGQYKVVYFTELQDHNKETEINRALS
                     GQHVYDGYLKNFGKDSAKEIIDGLLARMNQGEKVGAEEIERSLGSHLAR"
     gene            complement(5217..7067)
                     /locus_tag="VLE22_04970"
     CDS             complement(5217..7067)
                     /locus_tag="VLE22_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13788.1"
                     /translation="MPIRLLASALGLLIIAAPVMAAKTFVFHNAIRDLDEFRAHAGIA
                     ARLKSYGTVQVDIGVLAEKSPVHIPGVRSPWHDYGAYMATMWAFFPHPKLAAHLPAGW
                     VAKNRALLLAKVAILKELGLEAVFSANETQFLPESFFRQYPHLRGPRVDHPRRSGKDE
                     FAWCVDQPETLEMIEWMMAELKRNAPMIRAMHSWNNDSGSGLCWLRALYPGPNGPAYC
                     RGRDAGERIRGLIEAMDRGARKGGGPVHIRLAGNFPGEDREKIEPVLPSCARFRTGDK
                     SIIPVSTRVSEAYPVRGLIDLLPMLGALEKLPDPEVQTIDIDTCQSWYYRTDEPLSIV
                     KRVVDIVEDSIKSPARNLPDPTRKARTLVERWGGEKNADRLLEGFELVKQSFSVFSRG
                     FFREVVCSPGYAYMATNRLMTRPLVAKPELLTRQEEAYFLPFIFSTDENDARLDYNTA
                     HGDRRGGVSEYRSAVFRSIHDTAIEAAGIFEQAADAPEGAWFRQLGLSLRLWASMVRS
                     HDNFYFGQEIRDRRKDELAMPPRFLLAQMRDPDLLLWNEIERDELDNTNDLLVMLRNG
                     GLELIARARTPQEEDVFLYGPDVLGALRKKADIMRAHWLDGQRYLVPPRK"
     gene            7158..8687
                     /locus_tag="VLE22_04975"
     CDS             7158..8687
                     /locus_tag="VLE22_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HSB13789.1"
                     /translation="MQRRSFLQLAGGACASPLLSGNVLTAAVRQPNIVLIYADDLGFG
                     DLGCYGATRVKTPNLDRLAASGVRFTDAHSSSATCTPSRYSLLTGEYAFRKEGARVLP
                     GDAPLLIEPGRVTLASMLRSQGYRTGVVGKWHLGLGSGNVDWNGEIKPGPLELGFDYS
                     FLIPATGDRVPCVYVENHRVAGLDPKDPIRVSYKEKVGDEPTGRDNPELLQMHPSHGH
                     DMTIVNGISRIGYMSGGKAARWVDQDMADVITGKATGFIRQSKTQPFFLYFATHDIHV
                     PRVPHRRFAGNTPMGPRGDVIAELDWCVGQVLAEIERQNLAENTLVIFSSDNGPVVDD
                     GYKDQAVEKLGDHRPPGPYRGGKYSIFEAGTRIPLIVNWPRRVKPAVSTALVSQVDFL
                     ASFAGLTGAKLPQNAGPDSFDVMPALLGEAKSGRNHLVEHAGVLSLRKGNWKLILPGK
                     GARFNPNVNIELGNDPAPQLYDLSGDEVERKNLAAEHPGKVKEMIAMLEGIRKDGRSR
                     P"
     gene            8881..9843
                     /locus_tag="VLE22_04980"
     CDS             8881..9843
                     /locus_tag="VLE22_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13790.1"
                     /translation="MIGALCTGSSHGAPRVNPKRQPWSITVGYRFQPSSRHFVGTVEQ
                     KQRELNHNQIQNTYHLFDVSVTRQMGRRWALNGSLPILYATRNQLYVPRGDFRVAGLG
                     DMTLGGRAWLFRPPTESGANVGIGLSLKLPTGVYNATGRALDRQGNEIIATADQSIQA
                     GDGGTGFALDLQAYRPAWYRTMLYFSGTYLFNPRNTNGVSTFRTRPGEEVMSVADQYL
                     FRGGVSRAVPKVRGLTVTFGGRIEGVPVRDALGKSDGFRRPGYAISADPGFLYSTGSY
                     VFSCNVPFALERNRRKSVTDYQNGVHGDAAFADYSLILAVSRRF"
     gene            complement(9814..13044)
                     /locus_tag="VLE22_04985"
     CDS             complement(9814..13044)
                     /locus_tag="VLE22_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13791.1"
                     /translation="MNSLDQLNAYLARLESRLRMAAWVRGLALAAAVALIATLLLVLA
                     ANGYAFAERVVSGARVALFLSVALAAAFGLAVPLLRLNRRIAAREAETKFPEFDQRLL
                     TLAERGGDEQAGPFVDLLAADTLRLAEQAEPKRVVTSRRLLALASVATIAGGMLLWMG
                     LAGPGFLGYGTSLLWAGPSKEGVSPFYDIVIEPGNRSVRRGASQAVTARLVGFDAPQV
                     RLFARYRSASKWEEAPMLPKEGGAGYEFLFAGIPETVDYYAAAGRVRSKTHTLSVRDL
                     PSVKRIRVTYRFPDWTGQGTVVEDPGGDLSAVEGTGAEIAAETDRPLEGGLLVVDGSA
                     GTDLNRREGNWSSGQLTIRNNGHYYVAARDQGELVRLSDDFFIEARKDSEPVLRIVRP
                     GRDAKVSPIEEVTIAVEAEDDFGIQDMSLRYSVNGGPEKTLPLPAQKGAKQAEGSAVI
                     ALEDYRLVPGDVVTLYATARDARTAAKTDMYFLEAQPFEREYSQSQQMSSGGGEGGAE
                     NRISQRQKEIIAATWNQLRDGSRDGKSPADNARFLAEVQRTLGDQARSLARRMQSRQL
                     SSTNQEFQSFSADMEKAAGAMDEAAGKLRGARWQDALAPEQKALQHLLRAEATFRQIQ
                     VAFGNQRGGQGGGSGRELESLFDLELDTEKNQYETGQQTPSASQRNREVDETLQRLEQ
                     LARRQQELAGRERQAQQSSQQRWQQEMLRREAEELQKRMEQLSRGSGQSSGDQRLEQA
                     ARRLGEATRDMRQAGGSQGSGDAQARRAAERLREARDLMRGLRSEKSSGQLDDLARMA
                     EDLAKRQREFDGRLQQAFGGPAPEQEGRMVRPRLTPEQAEQSRQLAEEKRQMMDDLAR
                     LERDMQDATRDLAGSQPAAAGKLRRALGNMQKEELALRMRYGNELLRRGYGQQAVPRE
                     APITKGLEALRDELREARGALAQGPSGQGNATRALAEVERLRGRLGAGPGRLGERVFR
                     ESLRDLVRLRQSLSDIPEAAGDVEEIIGEMQRFNPQRVDEIASRILPALETMELRLRR
                     KLDEEQGGQVRSGAPDPVPPGYGDAVAEYFRRLSSRKSEPSAHRQD"
     gene            complement(13041..15044)
                     /locus_tag="VLE22_04990"
     CDS             complement(13041..15044)
                     /locus_tag="VLE22_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HSB13792.1"
                     /translation="MGFLTPWYLLGLVALGLPVWFHLLKKHKSEPVSFSSLMFFERRT
                     QSSILHRRLQHLLLFALRAALVAALVLAFAAPYLPGVAGTTGDRRMAVLAIDRSFSMR
                     QGSRLTEAKQKAVEVLAGLPSGSPAQVLEFAGQARLAGEPSSDKAALRAAIETVSASD
                     SRSSYADLVRALRSVAQSSRLPLDIHLFSDMQKTSLPAGFGDLQLPGDSRLSVHSVAA
                     ERLPNWTVESVTAPDRIFDPKRTRVQATVAGFGTGAAERQVSLVVNGKVLDTKRVAVP
                     VGGRAIVEFPSLDAPFGMSRCEVRIDAADSFPADDRFLFAVERADPRRVLFLHEPSDT
                     RSAVYFRAALEASAEAAFALDAVPVPQSGGVVPSKYSFVVLSNVASIPANFESELRSF
                     VRGGGALLIALGPSSAVRGKVPVVDLGLAESRYSSRRGERFQTAEWLDSGHPSVRRAN
                     RWDGVRFYQVVRADAGDGRVVARNSDRTPLLFEKTLGAGRVLVFASTFDNISNDFPLH
                     ASFVPFVEQTAYYLAGLEERRGGFTVDAYVPLSASRGSRAAVEVIGPDGNRMLELSES
                     SFVRDVPLAREGFYEIRHSSGGPALVAANADRLESDFEMIPAETLELWRSTGQGAAAR
                     GGKDETGAKPQHLWWYAMLVVLALALAESLVAGRYLGPEGETA"
     gene            complement(15197..16084)
                     /locus_tag="VLE22_04995"
     CDS             complement(15197..16084)
                     /locus_tag="VLE22_04995"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSB13793.1"
                     /translation="MFQRLLDPEVLAGISGLDLVAKTVVEGFIAGLHRSPDFGFSQEF
                     AEYRAYTQGDDLRHVDWNVFARTERAYLKRYRGETNTRLTLLLDASASMSYGSHLVSK
                     LDYARYLAASLFYLAHHQRDAAGLLVFDDEVRNYLNPSTRHGQLSRLLHALDKAVAGT
                     RTDFEKPFFHFQQFLYRPGLVVVVSDFYEDPDKVVTTIQPLRFRGNELILFHVLDPEE
                     IAPQFGRPVLLRDMETSDTMEVSPEYVQNEYRQKIDAHIEELRTKARSAGLDYFLLNT
                     ARPLDEALREYLVVRKGRM"
     gene            complement(16087..17112)
                     /locus_tag="VLE22_05000"
     CDS             complement(16087..17112)
                     /locus_tag="VLE22_05000"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSB13794.1"
                     /translation="MSTLTTQLDSAELKTRISRFQEVHEEILAQVRRVIVGQQEVLDQ
                     ILISLFVGGHCLITGLPGTAKTLMVRTIAQTLGLVFKRIQFTPDLMPSDITGTDIIEE
                     DTATGHRTWTFVPGPIFANVLLADEINRTPPKTQSALLEAMQELSVTVRGNHYDISRP
                     FFVLATQNPIELEGTYPLPEAQLDRFLFNTLLDYLSADEEMQVIDLTTTTHVAQADPV
                     TNAEEIVGFQQLVRMVPVAESVARYAVDLVRATRPSDPGAPDFVRKYVNFGGSVRAAQ
                     FLVLAAKARALMRRRYHVAFDDVAALVTPVLRHRLLLNFYAESDRLKPDDILRRLLDA
                     KPAPRGD"
     gene            complement(17132..19303)
                     /locus_tag="VLE22_05005"
     CDS             complement(17132..19303)
                     /locus_tag="VLE22_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB13795.1"
                     /translation="MTRGFLLLLLAVPLMAQTLDVCRAHRHHGRLQEASACFEKLAAS
                     PDAYLRAEGFWALGRHRQANDAFREAVAREPKSAVYRVRWGRLFHERMQRQDAAKLFQ
                     EALEISPGNPGALLGLALVASDGYERKAVELAERAAGLDAKFAEPRELLARLALEDGN
                     PRKAAEEADMALSIDPESLDAMAIRAAIDWLEDKPGTPWIGRVLKVNPVYGEAYALAG
                     HFLVLNRRYEEGIQFYRKALKLNPALLRARAELGVNLMRLGQDEEARWHLEYCYNNGD
                     QYAGVVNPLRLLDSHKHFVVLKSGQAVLKLHKRDADLLKPYFEAELARAIRTYERKYQ
                     MRLDRPVELQVYPDHEDFAVRTLGMPGLGALGVTFGFVVAMDSPNGRKPGTFHWASTL
                     WHELSHVFVLTATKHRVPRWFTEGMAVHEETAVSPDWGDRLSPEVIAAIREKKLLPIA
                     QLDRGFLRPSYPAQIVVSYFQAGRICDFINEKWGYAKLLDMMRSFGERKTTPEVVEQH
                     LGVKPEEFDQRFLSWLDGSTGKTVRGFDEWKTGLKAVAMLVKGGRHDDAIREGLAIRD
                     IYPDFVENGSVYELLAEAYLAKGDKPAAIAELSRYAKTGGRSPETIKQLASLLEEAGK
                     KREAAEALDRLNYIYPRDEDLHRRLGDLWLSVGDTQGAIREYQALVAMRPIDRATSHF
                     SLARAYRVANRVDEAREQVLLSLEAAPGYRPAQKLLLELSQ"
     gene            complement(19300..20223)
                     /locus_tag="VLE22_05010"
     CDS             complement(19300..20223)
                     /locus_tag="VLE22_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13796.1"
                     /translation="MRLAGLLLCSSLSLPAGTCYLTVSGLGGEPDYEQRFVSLAKTVD
                     KILRESSGEARVETLYGPAATRERVRAALEAFSRDCRPDDALVVMLIGHGTFDGVDYK
                     FNLPGPDLSAIELAGLLDRVPTSRQLVVNMTSASGGALDTLGKENRVVITATKSGTEK
                     NATVFARYWVEALRDAAADTDKNESVSALEAFRYADSKTVEFYDSQKRLATEHAMLDD
                     TGARQGVRAPSTENGQGSLAGSFTLVRFGSAQAAANSPEKRELLAKREELERQIDLLK
                     YRKAAMPSDDYRKQLGALLLDLARTQEEIDR"
     gene            complement(20220..22481)
                     /locus_tag="VLE22_05015"
     CDS             complement(20220..22481)
                     /locus_tag="VLE22_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13797.1"
                     /translation="MFEFFFKYSPTVYEKGSFVLLSRYPVWVLAVAVSMASGLLGWLI
                     WRRRERLAAGLSGWRPAAVWALQSSLAALLLLLLWHPAISVATLKPQQNIVAIVVDDS
                     RSMALREDGGTRREQAAAVLKGGLVAGLRKRFQVRMYRFGERLERIGEIGQVTAGAQA
                     THLGDSLRQLSAESAALPIGAVVLLSDGADNSGGIGLETISEIRRRRIPVHAIGFGRE
                     QFARDVELSDVTVPARVLADSRLSAQVTLRQHGYAQQKARLTVSDAGKVLASREIVFG
                     AEGEPQVETVDFNAGVAGARGLDVAVAPLAGEENLNNNMVARLINVEAFRPRLLYLEG
                     DPRWELKFIRRAVEEDRSLQITSILRTTQNKIYVQGVDKPKEFEDGFPTAAEVLFRFQ
                     GLIIGDVEASYFTPAQQELVRQFADRRGGGVLFLGGRAALADGGYGQSPLADLLPVVL
                     PTRKGTFRRDPATVELTAAGRDSLICRLEEEAERNAQRWKKLPALADYQEAGSAKPGA
                     LVLAESVVGGRQRSPLLVTQNFGRGRSAVMATGGSWRWKMLQEHTDRMHEMFWQQLLR
                     WLVAESPSQVTVSTPRQVIADEARIPLRAEVRDKAYAPAADARVEARILGPDGASASV
                     TLAPAQGEPGVYQGEWAAEKPGSYLVEAAATRGAEELGRDVMAFRREDGVAENFRAEQ
                     NRELLEKLADETGGRYYRPQDAAKLTDEISYSEAGITTREARDLWDMPALFLALIALK
                     SAEWLLRRRWGVV"
     gene            complement(22509..23315)
                     /locus_tag="VLE22_05020"
     CDS             complement(22509..23315)
                     /locus_tag="VLE22_05020"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HSB13798.1"
                     /translation="MRARTFTPVVLLLLLAVCAALALQESRLRYFLREEDNPAPIPPD
                     ANEKTEFAFARLRYNSIRSGGYWGFRGSWTTDYPKADRQFVQGVRRLTRIHTRSVEQV
                     VDLESDDIFNYPFVYAVETGHWDLTDAHAKKLREYLLRGGFLMTDDFHGTWEWDIFMS
                     SLRRVFPDRPVVDIGNDDAIFHVLYDLDERFQVPGIIMFRTGVTYEQDGVEPLWRGIY
                     DDRGRLMVAVCANMDLGDAWEWADMPQYPERYASLAYRVGINYIIYSMTH"
     gene            complement(23377..24348)
                     /locus_tag="VLE22_05025"
     CDS             complement(23377..24348)
                     /locus_tag="VLE22_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB13799.1"
                     /translation="MDPQIRPFEMVSETDEEVVVRTGFGAMIRKRFDAPMPAFLRFET
                     DSIEKMRAFSFDDPWDERRFFRGGDNQIAGVGDGFTRDLAPWIDIVKSLHPDFPVYGS
                     VCEGHETLWRIIGSENVMLWIGEYPDEVARFVERINAFSLELAKAQIKAAGGMLDGMV
                     IWGDVAYKKGTFFSPEYWRKYFKPGVKAIIDACHANGLPVIYHGCGNVNRIFEDFIEI
                     GVDAYNPLEAKAGLDVVDLRRRYGHRIGFCGNMDVLLWANGTREELKRAVLTKLNAAK
                     GGGYIFQSDHSVPGNVSAANYEYVVDLIRERGRYPLELGEYDLPDLS"
     gene            24602..24802
                     /locus_tag="VLE22_05030"
     CDS             24602..24802
                     /locus_tag="VLE22_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HSB13800.1"
                     /translation="MGAKAKPQIILRDGKPAAVILDIDKYRQLLERAEDAEDLRTLTS
                     MRRQKLKFRRLDDVLKEIGSRA"
     gene            24795..25049
                     /locus_tag="VLE22_05035"
     CDS             24795..25049
                     /locus_tag="VLE22_05035"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSB13801.1"
                     /translation="MHEILVSSQAGRDLRRAPAVILQRVVRAIKALSNDPRPSGARKI
                     TGSKNDWRIRIGDYRVLYELNDTERTVRVMRVRHRREVYR"
     gene            complement(25083..25943)
                     /locus_tag="VLE22_05040"
     CDS             complement(25083..25943)
                     /locus_tag="VLE22_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB13802.1"
                     /translation="MDLKLPIAAITDEFSPDLEVAVDAMSKIGMTGAELRVLWGKNIM
                     DLTDAELDRAKQILDGKGLQVMSIASPLLKCVLPDSPEIDSRFQHDIFASKHTYEGQP
                     RLAAHAFEIAHRTGAKIIRVFSYWRTVEPLKCFDRIVTALGELAEKASKEDLIIGLEN
                     EHACNLATGAEAAAALRAVDHPNLKLVWDPANALVSGEVPFPDGYRLLPANRIAHVHA
                     KDCYIKGHKPEWGPLGACGIDWKGQIAALLADGYRGYISLETHWAGPAGDKLQGSTIC
                     GWNMKNLVAA"
     gene            complement(25988..26452)
                     /locus_tag="VLE22_05045"
     CDS             complement(25988..26452)
                     /locus_tag="VLE22_05045"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HSB13803.1"
                     /translation="MIDTPQITQSTAQLTAVIGLIIPRAEIRNVMGPAISEVMATVAA
                     QGIAPAGPVFSYHFRMDPDTFDFEVGVPVAKPISPAGRVKPGELPAKKVARTVYHGGY
                     EGLGAAWGEFGAWIASNGHKPGPDLWEFYVAGPESSPDPANWRTELNRPLIR"
     gene            complement(26494..>27276)
                     /locus_tag="VLE22_05050"
     CDS             complement(26494..>27276)
                     /locus_tag="VLE22_05050"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB13804.1"
                     /translation="GAMGVDAADYDRSGRPHLLVGNFSNQMLGLYHNEGNGLFVDEAP
                     RSAVGKASLLSLAFGVFFFDYDLDGLPDLFAANGHIEEEIGRVQPKIQFQQPPLLFHN
                     LGKGRFEEVGTGAGQSFKRPIVARGAAYGDYDRDGDLDLLISTNHGPAYLFRNDGGNR
                     NNWLCVKAVGAKANRSGIGAVLRVESASGRQWQIVHSGSSYCSQSDLALTFGLGKDPI
                     VTVLEVTWPGGSKERFTKIKPNQFLTVVEGKGIMSPPGRSGS"
BASE COUNT         4969 a         8673 c         8633 g         5001 t
ORIGIN      
        1 gtcgccgaat cccggctctt cggaggagaa ctaccgcgac aatccaacgc cggacacgaa
       61 gggcaaagtg gtcgtgagcg acacggatca cctgtggggc cacacgggcg gcgacgcggt
      121 gtgggtgtgg aagagcttca cccgggggct gaacgtgctg ctgatggagg agttcacgcc
      181 gtcgccggcg tggcaggatt cggcgcggga ggcgatggga caggcacggc gctacgcgga
      241 gaagatggac ctggcggcga tgacgccggc tgccgaactg tgctcgacgg gatactgcct
      301 ggcggcgaag ggacgggagt acctggcatt catgccagga agtccggggc aattcaacgt
      361 gaacctgacg gacgtcgcgg gaggctgctc ggcggagtgg ctgaacgtaa caaccgggaa
      421 gaccgtgccg ggacaggaag tgcgcggcgg cgcgcggcgg acgttcgcga cgccgttcgg
      481 cgggccggcg gtgctctatt tgaaatgcgg gggctgaggg gaatctagaa ccgcacgcga
      541 gtgcacgccg gattctggct ttatttgcgt gcgttcccgg gaaactcggc ggccgcacgg
      601 aacatcgcga gcacgttcgc gtccggggtc ccgtagtcca tgttgatgca gcagacgccg
      661 acgttggcgg tccgtccggc ggcgcgaagc agttcgatgg tgtcgcggcg gatcgtttcg
      721 gacgattcct ggagcatgcg gaccgggctc agccggagat tgaggaacgc ttccgggaag
      781 actcgtgcac aggccgcgac gtccgatccc cagccgacgt caaggaacct tacgggaagt
      841 cgcgagtagc tttgcgcgaa caggtggaga ttgttcccgc agtggtggat gccgtaaggc
      901 gttagccttt cggccagcaa ctgctcgtgc ggaaggatag cccgctcgta gagtgacggg
      961 gagatcatct gcacggagca gcttgcgtgc aggtagagac ccgggtccac atgaagaatg
     1021 ccgcggttca ccgagaccga tgaggtcccg gtccgggacc gaacgtaatt cgccacgcgg
     1081 agttgggttt ccgcgattac ggtgaacacg tgatcggtag tctcggggtc ttcgaggagg
     1141 tcgaggaaga agtcgtttcc tcggagctcg agggcggtgt tgatgagacc gccggtgttg
     1201 aagtcgccgg ttagccggcc gcactctttc tcgaggccat ccatcagagc gctccactcg
     1261 ttcatcggcc aggttgactc gatctccggt acgcgaaggc ggagcgcctc ctcccggctc
     1321 aggttgcgcg gcacgttcca gggcgcctga ttgtcagaga accggatctc gacaccgagc
     1381 agggcgggta cgacgaaccc gcccgcaaca tggcgcgagc cggcgatggg gcgaggctga
     1441 gggtcggctt ctccgaggcc gaagcggtcg taaagtgccc ggcgcatgac aacgtcgtta
     1501 cggacgatgg tctccctgtg gaaatagaag ggacggtcga aggagacgtc gtagttccga
     1561 aaccaccagt tggggttgaa gacgacggag acgggaatgg acatgggccg gacggatggt
     1621 ctgaggtgct tccgtcgtag ccagtatacg ggatcggcgg aaagctggga catcccgccg
     1681 cggggttgtg tcccgggaac acagaaagta cttcttcggt tcctttaagc cttttagttt
     1741 caacacgcta agatggaaat aagggtgcaa ggaggagagc tggtatctgt atgaagcggc
     1801 tcgtgtggat cacgctttgg cttctggcgc cgatcttcgt accggcggcg acgctcgaac
     1861 ggttgtcgct ggacgagatg atcgaaaagt ccaccgcgat tgtgcggggc aagatcgttt
     1921 cggcgggggc gtcgttccac ggcccggtca tttacaccca cttttcggtt caggttctcg
     1981 aacactggaa gggcgacgac gtcgcgcagg ttgacgtcgt tgtgccgggc gggacggcca
     2041 agggtctcca ccagacgttc gcggggacgc ctcaactcga gcccggaggc gagtacgttc
     2101 tgttcctgtg gaaaggtaag agcggactga cgcacgtgat cgggctgtcg cagggcgtgt
     2161 tcacgttgaa gagcgacggc gatggcggca tgctggccac gcgctcggcg agcacggagg
     2221 cgatgctcga ccggcggacg ggccaggtgg tcaacgacga gacgcttcga ctgcgggtag
     2281 gcgatttgcg cgcgcgtgtc agcgcggcgg cgcgcgcgag gtgacggatt taaacatggt
     2341 tgcggcgcga aaactgatgg tggcggcggt ggcggcgact ctgctctccg gaaccgcgtt
     2401 tgcgcactat catttcctgc actacaagag ccgcaacgct ccatttgctc cggtccctga
     2461 gaagttcgac ctcaaggccc tcccaaatca gaccctgttc tacttcattt ctgaccaggg
     2521 accggagcag atggcgcctg gcgacagccg cgcatcgttc ctcagccaga tacggatggc
     2581 cgccgacgta tggagcggag tggaaacgtc ggagctgcgg ctggagttcg gcggcctgtt
     2641 cactcccggt gcgctgcaag cgaatccggg agtggacatt ttgttcgacg agattcctcc
     2701 cggcctggtg gcgatgggcg ggccgacggt tcgggcgtcg atggtggatg ccgaagcgaa
     2761 ctcgttcgtt ccgatcctgc gctcggtgct gatccttcag aaggatcttt cgcagcggcc
     2821 aagttacacc gattccttct tccagacggt ggtccacgaa atgggccatg cgctcggcct
     2881 gcagcacacg atgacttcga gcgttatggc gacgcaggtg acgagggcga cgacgaagtc
     2941 ccgcccgctg gcggcggacg acgtcgccgg gttatcggcg ctgtatccgg cgcaagcatt
     3001 cgccgagcgg acgggttcgg tcagcgggcg tgttgcgacg gacggtgtgg gtgtgcatct
     3061 cgcgtcggtg gtcgcggtgg cgccgaacgg cacggcggtg agtacgctga cggatccgga
     3121 tggccactac cggctggtcg ggctgacccc gggccagtat tacctttacg tccatccact
     3181 gccgccggcc gcgcagcagg ggttgggtcc ggcggacgtt gtccttccgg tggacccgga
     3241 tggtcagccg ttcgaggccg gtccggtctt tgaaacagtg ttctaccctg gaacgaagaa
     3301 tatccaggcg gccgccgtgc tcggggtcca ggccggcgtc acgctggaag gctacgactt
     3361 cccggtgacg aggcgcggcg ggctcgacct gttcggcgtc acaacctaca gttttcctgg
     3421 caattatgca gtgaagccgg cgttcatcaa cgtcaacggc ccccgcaatt tccttgtggc
     3481 ctccggcgtg gggctgacta cggatagcgc tccttcgccc ggactgacgg cggcggtgat
     3541 gggcggtaca gcgctgattc cggaaggcgg cataaaggct tactctccgg cgccggtgtt
     3601 cctccaactg gacttcgggt tcaatccgtt ctccggggag ggaccgaggc acctgctgtt
     3661 ctcgcgcaac aacgaccttt acgttttgcc ggccggactg aacctcgtga acaagcagcc
     3721 gccgtcgatt acggcagtgt ctccggcgct cgacggttcg ggaaaccgcg tggccatcct
     3781 gagcgggagc aaccttacac gcgacactcg gattctgttc gacggcctgc gggcgcaggt
     3841 agtgtcggcg gacgacacag cgggaacact gacggtagtt cctccggttg gcggcccgaa
     3901 ccaccaggca gtggtcacag cgctgaacgg cgacgggcag acatcgatgt tcctggatgc
     3961 agcggctccg ccgcagtata agtacgatgc cgtcgaggcg gcgccatatc tctcggtgtt
     4021 gccctcttcc ctgcccgccg ggtcggaagc gatggttgat atctacggcg caaacacgca
     4081 tttcacgccc gggcagacgg tgatcggctt cggcacgagc gatattgtcg ttcgccgtat
     4141 gtgggtgctc agcccggacc gcctgagggc caatgttcat gtcggcacgc tggccgcgga
     4201 agggatcgca aggctgacgg tagttacggg cttcgagatc gtcgaacatc ccttcgccct
     4261 cctagtgcag ccggcaaacc cagcagcatt cgtggtcaat cccagggcgg tgaaccccat
     4321 gaccggcttg cccagcatct acgcgggcgg ccaggcggcg gtcgaagtcg cgaaccttcc
     4381 agccggtgct gcggtcgcag tgaccctgaa cgatcagccg gcgacggtgg tttcggtgga
     4441 agatggccgg ctgatattcg ttgtcccggc gggcctcggc accggaacgg cgatcctgcg
     4501 aatttcggtg ggtacggtgg ccgcttatcc ggtggtccta gtgctcgatg cggcgccgcc
     4561 ggcagtgctg gcgatttcga aatccccggt tgtgccaggt gaggagcttg tggtgacggt
     4621 agccgggctc ggagatccgg gcgtcatcgg ctccccgcga aaggtgcgaa tcctcgtcgg
     4681 aggcgtcgaa catgccgccg ccgaagtgac gccctccgag agtgatccgg ccgtctacct
     4741 tgtgcgattc gtgctctccc ccgccgtgcc ccaaggcgac caggtgccgc tgatcgtgcg
     4801 catcggtgac cgggcgtcgc tgccggttcc gatcgccgtt cacagcccag agcagccccc
     4861 gaccgatagc ctgtaacgcg ttggttcccg cgccggctgt ggtaatccta aacatatatg
     4921 gcgtttgaag cggacaagac aatgttcgag atctaccgcg agaccgcgta ctctgggcag
     4981 tacaaggtcg tttacttcac ggagcttcag gaccacaaca aggaaacgga gatcaaccgc
     5041 gccttgtcgg gccagcacgt ctacgacggc tatctgaaga acttcggaaa ggacagcgcg
     5101 aaggagatca tcgacggcct gctcgcgcgg atgaaccaag gcgagaaggt aggcgccgaa
     5161 gagatcgaac gctccttagg cagccacctg gcgcgctgag cgcgggacgg cccagcctac
     5221 ttcctgggcg gtaccaggta tcgctgcccg tcgagccagt gggcccgcat gatgtccgcc
     5281 ttcttgcgga gggcgcccaa gacgtccggc ccgtacagaa agacgtcctc ctcctgtgga
     5341 gttcgggctc gcgcgatcag ttccaggccg ccgtttctca gcatgacgag gaggtcgttc
     5401 gtgttgtcca actcgtcccg ctcgatctcg ttccacaaaa gcaggtcagg atcgcgcatt
     5461 tgagccagca gaaaccgggg cggcatggca agctcatcct tgcgccggtc ccggatctct
     5521 tgcccaaagt agaaattgtc gtggctgcgg accatgctgg cccacagccg cagcgataac
     5581 cccaactgcc ggaaccacgc cccctcggga gcgtccgccg cctgctcgaa gattccggcc
     5641 gcctctatcg ccgtgtcgtg gatgcttcga aacacagcgg accggtattc gctcacaccg
     5701 ccccgtctgt caccgtgcgc cgtgttgtag tcgagacggg cgtcattctc gtccgtggaa
     5761 aagatgaacg gcagaaaata ggcttcctcc tgccgcgtca gcagttccgg tttggccacc
     5821 aacggtctcg tcatgagccg gttcgtggcc atgtaggcgt accccgggga acaaacgacc
     5881 tctcggaaga atccccgcga aaaaacgctg aatgactgct tcaccaactc gaagccttcg
     5941 agcaaccgat ccgcgttctt ttccccgccc cagcgttcga ccagcgtgcg ggctttcctg
     6001 gttgggtccg gcaggttccg ggcgggagac ttgatcgagt cctccacgat gtcaactact
     6061 cgcttgacga tcgagagtgg ttcgtcggtt ctgtaatacc aggactggca ggtgtcgatg
     6121 tcgattgtct gaacttcagg atcgggcagc ttttccaaag cccccagcat gggaagtaaa
     6181 tcaatgagac ctcgaacagg ataggcttcc gacactcttg tgctcaccgg gatgatcgac
     6241 ttgtccccgg ttctgaacct cgcacaggag ggcaggaccg gctcgatctt ctcgcggtcc
     6301 tcgccgggaa agttgcccgc cagccggatg tgcacgggcc cgccgccctt cctggcgcca
     6361 cggtccatcg cctcgatgag tcctcgaatc cgctctccgg cgtcgcggcc gcggcagtag
     6421 gcaggtccgt ttgggccagg gtacaacgct cgcaaccagc acaatcccga cccggagtcg
     6481 ttgttccagg aatgcattgc gcgaatcatc ggggcattcc gcttcagctc cgccatcatc
     6541 cactcgatca tctcgagcgt ctcgggctga tccacgcacc acgcaaactc gtctttcccg
     6601 cttcgacgcg gatgatccac gcgcggaccg cgcagatgcg gatattgccg gaagaacgat
     6661 tccggcagaa attgcgtctc attggcggag aagaccgcct cgagtcccag ttctttgagg
     6721 atagccacct ttgccagcaa cagcgcgcgg tttttcgcaa cccacccagc cggcaagtgg
     6781 gcggcgagtt tagggtgcgg gaagaatgcc cacatagtcg ccatgtaggc gccatagtca
     6841 tgccacgggc ttctcacacc cgggatatgg accgggctct tctccgccag gacaccgatg
     6901 tccacctgta ctgtgccgta actcttaagg cgggccgcta ttccggcgtg cgcgcggaac
     6961 tcatcgagat cgcgaatcgc gttgtgaaag acgaacgtct tggcagccat gacaggcgcc
     7021 gcgatgatga gcaaaccgag ggcgctagca agaagacgta ttggcatact cgcactgaga
     7081 atatcgatca aactcgagct atgcaaatcc ctaagccctt cggctgcacg aggggtatac
     7141 tgtgtaagcc taacgtcatg cagagacgct cttttctaca gctcgccggc ggcgcgtgcg
     7201 cctcgcccct actgagcgga aatgtgctca cagcggccgt gcggcaaccc aacatcgtgc
     7261 tgatctacgc cgacgacctc ggcttcggcg atctcgggtg ctacggcgcc accagagtca
     7321 agacgcccaa tctcgaccgg ctcgctgcat cgggcgtgcg tttcaccgat gcgcactcat
     7381 cgtcggcgac ctgcacgcct tcccggtact cgctgctcac cggtgagtac gcgtttcgga
     7441 aggaaggcgc gcgcgtgctg ccgggagacg caccgctgtt gatcgagccc ggccgcgtga
     7501 cgctcgcatc gatgctcagg agccagggct accgaacagg cgttgtcggc aagtggcacc
     7561 tgggccttgg cagcggcaac gtcgactgga acggcgagat caagccgggg ccgctcgaac
     7621 tcggcttcga ctactcattc ctcatcccgg ctacgggcga ccgtgtaccg tgcgtgtacg
     7681 tcgagaacca ccgggtcgcc ggactggacc cgaaggaccc gatccgggtc agctacaaag
     7741 agaaggtcgg cgacgagcca accggcaggg acaatccgga actgctccag atgcacccga
     7801 gccacgggca cgacatgacc atcgtgaacg gcatcagccg gatcgggtat atgagcggag
     7861 gcaaggctgc ccgttgggtg gaccaagaca tggccgacgt catcaccggc aaggccaccg
     7921 gcttcatccg gcagagcaag acgcaacctt tctttctgta cttcgctaca cacgacatcc
     7981 atgtgccgag ggtcccgcac agacgattcg ccggaaatac gccgatgggt ccgcgcggcg
     8041 acgtcatcgc agaattggac tggtgcgtgg gccaggttct ggcggagatc gaacgtcaga
     8101 acctggccga gaataccctt gtgattttct cgagcgacaa cggccccgtg gtcgacgacg
     8161 gttacaaaga tcaggcagtc gaaaaactcg gcgatcacag gccgcccggt ccataccgcg
     8221 gcggcaagta cagcatcttc gaggccggca cgcgaatccc gctgattgtg aactggccgc
     8281 gccgggtgaa gcccgccgtc tcgaccgccc tggtcagcca ggtggacttt ctggcatcct
     8341 tcgccggcct cacgggcgcg aagctgcccc aaaacgccgg accggacagc ttcgatgtga
     8401 tgcctgcgct gctcggtgag gccaagtcgg gccgcaatca cctcgtcgag cacgccgggg
     8461 tcctatcgct tcggaaggga aactggaagc tcatcctgcc aggtaagggc gccaggttca
     8521 atccaaatgt aaacattgaa ctcggcaacg accccgcgcc tcagctttac gacctctccg
     8581 gagacgaggt cgaaaggaag aacctcgcgg cggaacatcc cgggaaggta aaggaaatga
     8641 tagcgatgct cgaggggatc cggaaagatg gccggagtcg gccgtagtta actacttccg
     8701 ccctggacgc tgaaatacgg tcttgcccta accgcaatgg ctgagttatt ctaaagccag
     8761 ttaaggggct tctgggtggc tatccgttct gggtttctcg cctgttcgat cgcgcttgta
     8821 ctttgccttg cccgaccggc ttccggccag ggctgagtgg ccgcacgcca gagcgcgccc
     8881 gtgattggcg cgctatgcac gggatcgtcc cacggcgcac cgcgagtgaa tccgaagcgg
     8941 cagccttggt cgattactgt cggttaccgg ttccagccct cgtcgcgcca ctttgtgggt
     9001 acggtggagc agaagcagcg ggagctgaac cacaatcaga tccagaacac ttaccacctg
     9061 ttcgacgtgt cggtaacgcg gcagatggga cgccgctggg ccctgaatgg cagccttccg
     9121 attctttacg cgacgcgaaa ccaactgtac gtcccgcggg gagattttcg ggtggccggg
     9181 cttggggaca tgacgctcgg aggccgcgcg tggttgttcc gtccgcctac ggaatccggg
     9241 gcgaacgtgg gtatcggact tagcttgaag ctgccgacgg gcgtctataa cgccaccgga
     9301 cgcgcgctgg accgtcaggg caatgaaatc atcgccaccg cggatcagtc gatccaggcg
     9361 ggcgacgggg gcacaggatt tgcgctcgac ctccaggctt acagacccgc gtggtaccgc
     9421 accatgctct atttttcggg cacgtatctg ttcaatcccc ggaatacaaa cggcgtgtca
     9481 acgttccgga cgcggcccgg cgaagaggta atgtcggtgg cagaccagta cctgttccgt
     9541 ggcggagttt cccgtgccgt accgaaagtc cgcgggctga cggtgacctt cggcgggcgg
     9601 atcgagggcg tgcccgtgag ggacgcgcta ggcaagagcg acggcttccg gcggcccgga
     9661 tatgcgatct cggccgatcc cggtttcctt tactccaccg gctcatatgt gttctcgtgc
     9721 aacgtgccgt ttgcgttgga gcgcaatcgc aggaagagtg tgaccgacta tcagaacggt
     9781 gtgcacggcg atgcggcctt cgccgactac agtctaatcc tggcggtgag cagacggttc
     9841 tgacttccgg gaactgaggc ggcggaagta ctccgcgacg gcatcgccat agcccggcgg
     9901 aacgggatca ggcgccccag accgcacctg cccaccctgc tcttcatcaa gcttccgccg
     9961 gagccgcaac tccattgtct ccagcgcggg cagaatccgc gacgcaatct cgtcgacgcg
    10021 ctgcggattg aaacgctgca tctcgccgat gatctcctcg acgtctccag cggcctcggg
    10081 gatatccgaa agcgactgcc gcagccggac gagatcgcgg aggctttccc ggaagacccg
    10141 ctcgccgagc cgtccgggac cggcgccgag acggccgcga aggcgctcca cttcggcgag
    10201 cgcgcgagtc gcgttgccct ggcccgacgg gccttgcgcg agcgcgccgc gtgcttccct
    10261 gagttcgtcg cgcagcgcct ccagtccttt tgtaatcggc gcctcccgcg gcaccgcctg
    10321 ctggccgtaa ccgcgccgca ggagctcatt gccgtaccgc atccggagcg cgagttcctc
    10381 tttctgcata ttgccgagag cgcgccgtag tttcccggca gccgcgggct gcgaaccggc
    10441 gaggtcacgc gtggcgtcct gcatatcgcg ttccagacgg gcgaggtcgt ccatcatttg
    10501 ccgtttctcc tcggcaagct ggcgcgactg ctcagcctgt tccggtgtca gccgcgggcg
    10561 caccatccgg ccctcctgct ccggagccgg tccaccgaaa gcctgctgaa ggcgcccgtc
    10621 gaactcgcgc tgccgcttgg cgagatcttc tgccatccga gcgagatcgt ccaactgccc
    10681 ggagctcttc tcgcttcgca agccgcgcat gagatcgcgg gcttcccgaa gccgctcggc
    10741 ggcacggcgc gcttgagcgt cgccactccc ctgtgagccg ccggcctggc gcatgtctcg
    10801 ggtggcctcg cccagcctgc gagccgcctg ttcgaggcgc tggtctccgg aagattgccc
    10861 gcttcctctg gagagctgtt ccatgcgttt ctggagctcc tccgcctcgc gccggagcat
    10921 ctcttgctgc cagcgctgct gggaggactg ctgtgcctgc cgttcgcggc cggccaattc
    10981 ctgctgcctg cgggcgagtt gctccagccg ctgaagggtc tcgtcgacct cgcggtttct
    11041 ttggctcgcc gacggcgtct gctggccggt ctcgtactgg ttcttttcgg tgtcgagctc
    11101 gaggtcaaaa aggctctcga gttcgcgccc cgagcctccg ccctggccgc cacgctgatt
    11161 tccgaacgca acctggatct gacgaaaggt agcttctgcg cggaggagat gctggagcgc
    11221 tttctgctcc ggagcaagcg cgtcttgcca tcgggctccg cgcagtttgc cggcagcctc
    11281 gtccatcgca ccggcggcct tctccatatc cgctgaaaag ctttggaact cctggttggt
    11341 ggatgaaagc tggcggctct gcatgcgccg cgcgagcgac cgtgcctgat cgcccaacgt
    11401 cctctgcacc tcggcgagga aacgcgcgtt gtcggcgggt gattttccgt cgcgtgaacc
    11461 gtcgcgcaat tgattccaag tcgcggcaat gatctccttt tggcgttgcg agatgcggtt
    11521 ctcggcgccg ccttcccctc cgcccgagga catctgctgg gactggctgt actcacgttc
    11581 gaacggttgc gcctcaagga agtacatgtc cgtcttcgcc gcggtacggg cgtcgcgggc
    11641 ggtggcgtaa agggtaacaa cgtcgcccgg aacgagccgg tagtcttcga gcgcgatgac
    11701 ggcggaaccc tccgcctgtt tggcgccttt ttgtgccggc agcggcagcg tcttctccgg
    11761 cccgccattg accgaataac gcagcgacat atcctggatg ccgaagtcat cctcggcctc
    11821 aacggcgatg gtgacctcct cgatcgggct gaccttggca tcgcgccctg ggcgcacaat
    11881 gcgaagcaca ggctcactgt ccttgcgagc ttcgatgaag aagtcgtcgc tgagacggac
    11941 cagctcgccc tgatcgcggg cggcgacgta atagtgaccg ttgtttcgga tggtgagctg
    12001 tcctgaggac cagttccctt ccctccgatt gaggtctgtt ccagcggatc cgtcgacaac
    12061 gagcagcccg ccttcgagag gccggtcggt ctccgccgcg atttcagcgc cggtgccctc
    12121 caccgcgctg aggtcgcctc cgggatcctc cactactgtg ccctggccgg tccagtcggg
    12181 aaaacgatac gtgacgcgaa ttcgcttcac agagggcaga tctctgaccg acagagtgtg
    12241 ggtctttgat cgaacgcgtc cggcagccgc gtagtagtcc acggtttcgg gaataccggc
    12301 aaagaggaac tcatacccgg cgccgccttc cttgggaagc atcggcgctt cttcccattt
    12361 cgaggcgctt cgatagcggg cgaagagccg cacctggggc gcatcgaatc caacgagacg
    12421 tgcagtcaca gcctggctcg ctccccggcg cacggaacgg ttgccgggct cgatcacgat
    12481 gtcgtagaac ggcgagacgc cttctttgga tggtccggcc cacagtagcg aagtcccata
    12541 accgaggaag ccgggtccgg ccaagcccat ccagaggagc atgccgccgg cgatcgtagc
    12601 aaccgaggcg agggccagta gccgcctcga agtcaccacg cgtttcggtt cggcctgctc
    12661 ggccagtcgc aaggtatcgg cagctagaag gtcgacgaac ggtcccgctt gctcgtcccc
    12721 gccgcgctcg gccagtgtca gcaggcgctg gtcgaactcg gggaacttgg tttcggcctc
    12781 gcgagccgca atacgccggt tgagccgcag cagcgggacc gcgagaccga aggcggctgc
    12841 caacgcaacg ctcaggaaga gcgcaacacg ggcgccggac accacgcgct ccgcgaacgc
    12901 atagccgttc gcggccagaa cgagcagaag ggtcgcaatc agcgctacag ccgcagcaag
    12961 agccaggccg cgcacccacg ccgccatgcg gagacgagac tccaaccgtg ccaggtaagc
    13021 gttgagttgg tccagcgagt tcatgcggtt tccccctccg ggccgagata ccggcccgcg
    13081 accagcgact ccgcgagcgc aagcgcgaga acaaccaaca tcgcgtacca ccaaagatgc
    13141 tgcggcttgg cccctgtctc atcctttccg ccccgtgcgg ccgcgccctg ccccgtgctc
    13201 cgccagagtt cgagcgtttc ggccgggatc atctcgaagt cggactcgag ccggtcggcg
    13261 tttgcagcga ccagcgccgg tcccccgctg ctgtgccgga tttcgtagaa accctcccgt
    13321 gcgagcggaa cgtcgcgaac gaacgaagat tccgataatt cgagcatgcg gtttccatcg
    13381 ggcccgatta cttccaccgc ggcccgactt ccgcgagacg cactcagcgg gacgtaggcg
    13441 tccaccgtga acccaccgcg tcgttcttca aggcccgcga gatagtatgc ggtttgctcg
    13501 acgaacggca caaacgaggc atgcaacggg aaatcgttcg aaatgttatc gaaggtggag
    13561 gcaaagacca gcacacggcc ggcgcccaaa gtcttctcga atagcagggg tgtgcggtcg
    13621 ctattgcgtg ccacgactcg gccgtctccg gcgtcggcgc gaacgacctg ataaaaccgg
    13681 acaccgtccc aacggttggc gcgccgaacg gagggatgcc cggagtcgag ccactccgcg
    13741 gtctgaaaac gctcgccgcg gcgggaggag taacgggatt cagccagccc caagtcgacc
    13801 accggcacct tgcctctcac cgccgaggat ggtccgagag cgatcaggag cgcaccgccc
    13861 ccgcggacga aacttcggag ctcgctttcg aagttcgcgg ggatcgaagc gacgttggag
    13921 agcacgacga aactgtattt cgacggtacg acgccgccgg actgcggcac gggaaccgca
    13981 tcgagagcga acgctgcctc ggccgatgct tcgagcgcgg cgcggaaata aaccgccgac
    14041 cgtgtatcgc tcggctcatg gaggaacagt acacggcgcg ggtcggcccg ctcgacggca
    14101 aacaaaaagc ggtcgtctgc cgggaaggag tcggctgcgt cgatgcgaac ctcgcaacgg
    14161 ctcatcccga agggcgcgtc gagcgacgga aattcaacga tggcgcggcc tccaacgggc
    14221 actgcgactc tcttcgtgtc gagtaccttg ccattaacca caagggagac ctggcgctcg
    14281 gcggccccgg ttccgaagcc cgccacggtg gcctggaccc gcgtccgctt cggatcgaag
    14341 atccggtcag gggcggtgac actttccacc gtccagttcg gcagccgctc cgcggccacg
    14401 gagtgaacag acagccgaga gtctcccgga agttgtagat cgccgaagcc cgcgggcagc
    14461 gacgtcttct gcatgtcgct gaagagatgg atgtcgagtg gcagcctgga cgattgagcc
    14521 accgaccgga gcgcgcgcac gagatcggca taggagcttc gggagtccga agcagacaca
    14581 gtctcgatag ctgcgcgcag tgcggcctta tccgaggacg gttcgcccgc caggcgggcc
    14641 tgtccggcga actcgagcac ctgcgccggc gagcctgagg gcagaccggc gagcacctct
    14701 accgccttct gcttcgcttc ggtcagccgg ctgccctgcc gcatgctgaa tgagcggtca
    14761 atggcgagca cggccatccg gcgatcgccc gtcgttccgg ctacccccgg caggtacggc
    14821 gcggcgaagg ccaacacaag cgcggcaacg agggccgccc gcagggcgaa taggagaagg
    14881 tgctggagcc ggcggtgcag gatggagctt tgcgtccgcc gctcgaagaa catcagcgag
    14941 ctgaaggaaa ccggctcgct cttgtgcttc ttgagcagat ggaaccacac gggcagcccg
    15001 agggcgacca gccccagcag ataccatgga gtaagaaagc ccatcagctc atctcagaat
    15061 tctttcacca caactctgtc ttcaccacag aggacgcaaa ggacacagaa aaaagatgtt
    15121 tgccgatggc tctctgtggt ctctgtgccc cctgtggtga tgccttttca ccacaggtga
    15181 tgccttctac aaacatctac atcctccctt tcctcacaac taggtactcg cggagggctt
    15241 cgtcgagagg ccgggccgtg ttcagcagaa agtagtcgag gccagcgctc cgggccttcg
    15301 tccgcagctc ttcgatgtgc gcgtcgattt tttgcctgta ctcgttctgg acgtattcgg
    15361 gactcacctc catcgtgtcg ctggtttcca tgtcgcgaag caaaacgggc cggccgaatt
    15421 gcggggcaat ctcctccggg tcgagaacat gaaacaggat aagctcgttc cctcggaaac
    15481 gcagcggctg aatcgtcgtt acaaccttgt ccggatcctc atagaagtcc gagacaacca
    15541 ccacgagccc gggccggtac aggaactgct gaaaatggaa aaacggcttc tcgaaatccg
    15601 tgcgcgtgcc ggcaacagcc ttatccagtg cgtgcagaag ccggctgagc tggccgtggc
    15661 gcgtggacgg gttcagataa ttgcgtactt cgtcgtcgaa caccagcaga ccggccgcgt
    15721 cgcgctggtg atgggcgagg taaaatagcg atgccgccag gtaacgcgcg taatcgagct
    15781 tgctcacgag atgcgagccg tacgacatgg acgcgctcgc gtcgagaagc agcgtgagtc
    15841 gcgtgttcgt ctcgccacgg taccgcttga ggtaggcgcg ctcggttctg gcaaagacgt
    15901 tccaatccac gtgccgcaga tcgtcgccct gcgtgtaggc gcggtattcg gcgaactcct
    15961 ggctgaatcc gaagtcgggg gaacggtgca accccgcgat gaacccctcg accacggttt
    16021 tcgccacgag gtcgagtccc gagatgccgg ccagaacttc aggatcgagg agccgctgga
    16081 acatggtcag tctccacgtg gagccggttt cgcatcgagc aaccggcgga gaatgtcgtc
    16141 cggcttgagg cggtcggatt cggcatagaa gttcaacagg agccggtgcc ggagcaccgg
    16201 cgtcaccaga gccgcaacgt cgtcaaacgc cacgtgatag cggcgtcgca tcaaagcgcg
    16261 cgccttggcg gccaggacca ggaactgggc ggcgcgcacg ctgccgccga agttcacgta
    16321 cttcctaacg aaatccggcg cgccggggtc ggacgggcgc gtggcccgga cgaggtccac
    16381 cgcgtatcgc gccaccgact cagcgaccgg aaccatgcga acaagctgct gaaatccgac
    16441 gatctcctcc gcgttcgtga ccggatcggc ctgggcgacg tgggtcgtgg tcgtcagatc
    16501 gatgacctgc atctcttcgt ctgccgacag atagtcgagg agcgtgttaa acagaaaccg
    16561 gtcgagctgc gcttccggca gcgggtacgt gccctccagt tcaatcgggt tctgcgtggc
    16621 gaggacaaag aacggccgcg agatatcgta atgattgccg cgaacggtaa ccgaaagctc
    16681 ctgcatcgcc tcgaggagcg ccgactgcgt cttcggcggc gtacggttga tttcgtccgc
    16741 cagcagcacg ttggcaaaaa tcggccccgg cacgaacgtc caggtgcggt ggccggtggc
    16801 agtgtcctcc tcgatgatgt ccgtgcccgt gatatcggac ggcatcaggt ccggcgtgaa
    16861 ctggatgcgc ttgaaaacca gaccgagcgt ctgggcgatc gtgcgcacca tcagcgtctt
    16921 cgccgtcccg ggcaggccgg tgatcagaca gtgtccgcca acgaaaaggg aaatcaggat
    16981 ctggtcgagc acttcctgtt gccccacaat gactcgccgc acctgtgcca ggatctcctc
    17041 atgcacctcc tgaaaccggc tgatccgtgt tttcaattcc gctgaatcga gttgagtggt
    17101 cagtgttgac attccgtcca tccgctttcg cttattgaga gagctccagg agcaactttt
    17161 gcgcgggccg gtagcccggc gcggcttcga gcgacagcag cacttgctcg cgtgcctcgt
    17221 ccacgcggtt cgccacccgg tacgcccggg caagactgaa atgcgacgta gccctgtcga
    17281 tcgggcgcat cgccacaaga gcctggtact cgcgaatcgc accctgcgtg tcgcccacgg
    17341 acagccacag gtctccaagc cgccggtgca ggtcctcgtc gcgcgggtag atgtagttga
    17401 ggcggtcgag cgcctcggcg gcttcccttt tcttgcctgc ctcctccaac agtgatgcaa
    17461 gctgcttgat cgtctccggg ctgcgcccgc cggttttggc gtaacggctg agttccgcga
    17521 tggcagcagg cttgtcgccc ttcgcgaggt aagcctcggc gagcaactca tagacgctgc
    17581 cgttctccac gaaatccgga tagatgtcac gaatggcgag gccctcccgg atcgcgtcgt
    17641 cgtgacgccc gcctttgacg agcatggcaa cggcctttaa gcccgtcttc cattcgtcga
    17701 aaccgcggac ggtctttccg gtggagccgt caagccacga aaggaaacgc tggtcgaact
    17761 cctcgggctt cacgccgagg tgctgctcga ccacctcggg tgtggtcttt cgctcgccga
    17821 aagatcgcat catgtcgagc agcttcgcgt agccccattt ctcattgatg aagtcgcaga
    17881 ttctgcctgc ctggaagtac gagaccacaa tctgcgcggg gtacgatggc cgcaggaaac
    17941 cgcggtcgag ctgcgcgatt ggaaggagct tcttttcgcg gatggcggcg atcacttccg
    18001 gcgagaggcg gtcgccccaa tcgggcgata cggctgtctc ttcgtgaacg gccatgcctt
    18061 ccgtgaacca ccgcggtaca cgatgcttcg tcgcagtgag cacaaacacg tggctcagtt
    18121 catgccagag cgtgctggcc caatggaacg tgccgggctt gcggccgttc gggctatcca
    18181 tggcgacgac gaagccgaag gtcacgccaa gggctccgag ccccggcatg ccgagtgtgc
    18241 ggactgcgaa atcctcgtgg tccggataga cctgaagctc cacgggccgg tcgagccgca
    18301 tctggtactt gcgctcgtag gtgcgaatgg cgcgcgccag ttcggcctca aagtagggtt
    18361 tcagcagatc ggcgtcccgt ttgtggagtt tcagcaccgc ctggccggac ttcagaacga
    18421 cgaagtgctt gtggctgtcg agcagccgca gcggattgac cacgccggcg tactggtcgc
    18481 cgttgttgta acagtactcg aggtgccagc gcgcctcctc gtcctggccg agccgcatga
    18541 gattgacgcc aagctcggca cgggccctca gaagcgcggg attgagcttc agtgctttgc
    18601 ggtagaactg gatgccctct tcgtagcggc ggttcagcac gaggaaatgg ccggctagcg
    18661 cgtaggcttc gccgtacacg ggattgacct tcagcacgcg gccgatccag ggagtccccg
    18721 gcttgtcttc gagccagtca atcgccgcgc gaatggccat cgcgtccagc gattcggggt
    18781 cgatcgacaa cgccatgtcg gcctcctcgg cggccttacg cgggttgccg tcttcgagag
    18841 cgaggcgggc aagcaactcg cgcggctcgg cgaactttgc gtccagaccg gctgcgcgtt
    18901 cggccagttc gaccgccttc ctttcgtagc cgtcggaggc gaccagcgcc agacccagga
    18961 gcgctccagg gttcccgggg ctgatttcca gcgcctcctg gaagagcttc gcagcgtcct
    19021 gtctctgcat ccgctcgtgg aacaggcggc cccaccggac gcgataaacc gcactctttg
    19081 gttcccgcgc cacggcctcg cggaaagcat cattcgcctg ccggtgccgt ccgagcgccc
    19141 agaagccttc tgcacgcaga taggcgtccg gcgacgccgc gagcttttca aaacacgccg
    19201 aggcttcctg caaccgcccg tgatgccggt gtgcgcggca gacatcgagt gtctgcgcca
    19261 ttaacgggac ggccaacagt aagagcagga aaccgcgagt catcggtcaa tctcctcctg
    19321 cgtccgggca aggtcgagca gcagagcgcc aagctgtttg cgataatcgt ccgacggcat
    19381 ggcggctttg cggtacttga ggagatcgat ctgccgttcg agctcctctc tcttcgctag
    19441 aagttctcgt ttttccgggc tgttcgccgc ggcctgggcg ctgccgaagc gtaccagcgt
    19501 gaacgacccg gccaggcttc cctggccgtt ttcggtagat ggcgcgcgga ccccctgccg
    19561 ggccccggtg tcgtccagca tcgcgtgctc ggtggccaaa cgcttttgtg aatcgtagaa
    19621 ctcgacagtt ttgctgtccg cgtagcggaa ggcttccagt gcggacaccg actcgttctt
    19681 atccgtatcc gcggcggcgt cacgcaacgc ctccacccaa tagcgggcga acacggtcgc
    19741 gttcttctca gtgccgctct ttgtggcggt gatgacgacg cggttctcct tgccgagcgt
    19801 gtcgagggcg cccccgctgg cactggtcat gttcacgacc agttgccgcg aagttgggac
    19861 gcggtcgagc agccccgcga gctcaatggc cgatagatcc ggacccggca ggttgaactt
    19921 gtagtcgacg ccgtcgaacg tgccgtggcc gatcagcatg acgaccagcg cgtcatcagg
    19981 ccggcagtct cgtgagaagg cttcgagcgc agcacggaca cgctcgcgcg tggccgctgg
    20041 accgtaaagc gtctcgacgc gcgcctcccc actgctttcc cggagaatct tgtcgacggt
    20101 cttcgccagt gagacaaagc gttgctcgta gtccggctcg ccgccgaggc ccgaaacagt
    20161 gagatagcac gtacccgccg gaagcgagag gctggagcac agcaacagcc cagcgagtct
    20221 cataccaccc cccaacggcg acgcagcagc cactcggcgc ttttgagcgc gatcagcgcg
    20281 aggaacaacg ccggcatgtc ccatagatcg cgggcttccc gcgtggtaat gccggcttcg
    20341 gagtacgaga tttcatccgt cagcttcgcc gcatcctgtg ggcggtagta acggccgccg
    20401 gtctcatcgg cgagtttctc gagcagttcg cgattctgct cggcccggaa attctcggcg
    20461 acgccgtctt cgcggcggaa ggccatcacg tcgcgcccga gttcttccgc gccccttgtc
    20521 gccgccgcct ccacaagata ggaacccggc ttctctgcag cccattcgcc ctggtacacg
    20581 cccggctctc cctgagccgg cgcgagcgtc acactcgccg aagcgccgtc gggcccgagg
    20641 atgcgtgctt cgacgcgggc gtctgccgcc ggcgcgtatg ccttgtcccg tacttccgcc
    20701 cgaagaggga tgcgggcttc gtcggcgatc acctggcggg gcgtcgacac ggtgacctgc
    20761 gacggcgatt ccgccaccag ccagcgcagc aactgctgcc agaacatctc gtgcatgcgg
    20821 tcggtgtgtt cctggagcat cttccagcgc cagcttccgc cggttgccat caccgcggag
    20881 cgtccccggc cgaagttctg cgtcaccagc aacggcgacc gttgccttcc acccacaacc
    20941 gactcggcca gcacaagcgc gccaggcttc gcgctgcccg cctcctggta gtcggccagc
    21001 gccggtagtt tcttccaccg ttgcgcgttt cgctcggctt cctcctccag ccggcagatg
    21061 aggctgtcgc ggccggcggc ggtcagttcc acggtcgccg gatcgcgccg aaacgtcccc
    21121 tttcgcgtgg gcagtacgac ggggagcagg tccgccagcg gagactgccc atagccgccg
    21181 tcggcgagcg ccgctctccc tccgagaaag agaacgccgc cgccgcgccg gtcggcgaat
    21241 tgccgcacca actcctgttg ggccggggtg aagtagcttg cctcgacatc gccgatgatc
    21301 aaaccctgaa agcggaagag tacttcggcg gccgtcggaa agccgtcttc gaactccttc
    21361 ggcttgtcga cgccctgcac gtagatcttg ttctgcgtgg tgcgcaggat cgacgtgatc
    21421 tggagactgc ggtcctcctc gaccgcgcgg cgtatgaact tcagctccca gcgcggatcg
    21481 ccctccaggt acaggagtct cggccggaag gcttccacgt tgatcagacg ggcgaccatg
    21541 ttgttgttca ggttttcctc gccggcgagc ggcgcgaccg ccacatccaa tccgcgggcc
    21601 ccggcaacgc ccgcattgaa atcaaccgtc tcgacctggg gctcgccctc cgcgccaaag
    21661 acgatctcgc gagaggcgag caccttgccg gcatcgctca cggtcaaccg cgccttctgc
    21721 tgcgcatagc cgtgctgccg gagcgtcacc tgcgcgctga gccgggaatc cgccagcacc
    21781 cgggccggca cggtgacgtc gctaagctcc acgtcacggg cgaactgctc ccttccgaat
    21841 ccgatcgcat gcaccgggat gcgccgccgc cggatctcgg agatcgtctc caggccgatg
    21901 ccgccgctgt tgtccgcgcc gtcgctcagc agcactaccg caccgatcgg caatgcggcg
    21961 ctttcggcgg aaagttgtct cagtgaatcg ccgaggtgcg tcgcctgcgc cccggcggtg
    22021 acttggccga tctcgccgat cctctccagg cgctcgccga aacgatacat ccgcacctgg
    22081 aaccgcttcc gcagtccggc gaccagcccc cccttgagca ccgcggcggc ctgctcccgg
    22141 cgagtgccgc catcctctcg cagcgccatg ctccgtgaat cgtccaccac aatcgcgacg
    22201 atgttctgct gcggcttgag cgtcgccacg ctgatcgccg ggtgccacaa aagcagaagc
    22261 agcagggccg ccagcgaact ctggagcgcc cacacggccg ccggacgcca accggaaagg
    22321 ccggcagcca gcctctcgcg acgccgccag atgagccatc cgagcaggcc cgacgccatt
    22381 gatacggcca cggccaaaac ccagaccgga taacgcgaca gcaacacaaa gctccccttt
    22441 tcatacaccg tgggggaata cttaaagaaa aactcgaaca tgtcgcgctc gccgtttcgg
    22501 tttttttgct agtgcgtcat cgaataaatg atgtagttga tcccgacgcg gtaagcgagg
    22561 gatgcatagc gctccggata ctgtggcatg tcagcccact cccaggcgtc gccgaggtcc
    22621 atgttggcgc aaacggccac catcaggcgg ccccggtcgt catagatgcc ccgccagagc
    22681 ggctcgacgc catcctgctc ataggtgacg cccgtgcgga acatgataat gccgggcacc
    22741 tgaaaccgct cgtcgagatc atacaggacg tggaagatcg catcgtcgtt gccgatgtcg
    22801 acgaccggcc ggtccgggaa aacccgccgg aggctcgaca tgaagatgtc ccactcccac
    22861 gttccgtgga agtcgtcggt catgagaaag ccgccgcgaa ggaggtactc gcgaagtttc
    22921 ttcgcgtggg catcagtcag atcccagtgt cctgtctcca ccgcgtatac gaacggatag
    22981 ttgaagatat cgtcggactc aaggtcgacc acctgctcga ccgagcgcgt gtgtatccgc
    23041 gtaagccgcc tgacgccttg gacgaactgc cggtcggcct tcggataatc ggtcgtccac
    23101 gagccgcgaa agccccaata gccgccggat cgtatcgaat tgtaacggag gcgcgcgaag
    23161 gcgaattcgg tcttctcgtt agcgtcaggg ggaatcgggg cgggattgtc ttcctcccga
    23221 aggaaatagc gcaacctgct ctcttgcagc gcgagcgccg cgcacacagc gagcagaagg
    23281 agtaacacca ccggcgtgaa tgtccgagca cgcatcggcc ccccgcagat gaatccagaa
    23341 tagcagattc atgaacaccg gcactcagta aagcgctcag ctcaggtcgg gcagatcgta
    23401 ctcgccgagt tcaagcggat agcgccctcg ctcgcgaatg aggtcaacga cgtactcgta
    23461 gttcgcggcg ctgacgttgc ccggcaccga gtggtcggac tggaagatgt agccgccgcc
    23521 cttcgccgca ttgagcttgg tcaggactgc ccgcttcagt tcctcgcggg tgccgttcgc
    23581 ccagagcagc acgtccatgt tcccgcagaa gccgatgcga tgcccgtagc gccggcgcag
    23641 gtcgacgaca tccagtcccg ccttggcttc gagcgggttg taggcgtcca cgccgatctc
    23701 gatgaagtcc tcgaaaatgc gattgacgtt tccgcacccg tgataaatga caggcagacc
    23761 gttcgcgtgg caggcgtcga tgatcgcctt cacccccggc ttgaaatact tgcgccagta
    23821 ctcaggcgag aagaacgttc ctttcttgta tgctacgtcg ccccagatca ccatgccgtc
    23881 gagcatcccg ccggcggctt tgatctgcgc cttcgcgagt tcgagcgaga acgcattgat
    23941 ccgctcgacg aagcgggcta cttcatccgg gtattcgccg atccagagca tcacattctc
    24001 cgagccgatg atgcgccaga gcgtctcgtg tccctcacag acgctgccgt aaacggggaa
    24061 atcgggatga agcgacttca cgatgtcgat ccacggcgcg aggtcgcgcg tgaagccgtc
    24121 gccgacgccg gcaatctggt tgtcgccccc tcggaaaaag cggcgctcat cccacgggtc
    24181 gtcgaaagag aacgcccgca tcttctcgat gctgtcggtc tcaaaccgca ggaaagccgg
    24241 catcggggcg tcgaatcgct tccggatcat ggcgccgaag ccggtccgca caacgacctc
    24301 ttcgtccgtc tcggaaacca tctcgaatgg ccggatttgt ggatccatgt tggggatggt
    24361 gacttgccag tcgaggtcgt agtagcggta gatgtccgcg tccgccgcga gacccagctc
    24421 ttccctccac cgcctgagga agctccccca gaaaaaagtc gctgaccggg acgcggtccg
    24481 gctcctcgtg ccgcagggcc ttgttcatcc gctcgagctt ggcgaggcaa ttggaggtgc
    24541 ggggcatgag aaggtgattt taccgctgtc gcaatccgta gccatgggat aaactcgtgc
    24601 tatgggtgcc aaagcaaagc cccaaattat tctgcgagac ggaaagccgg cggccgtgat
    24661 cctcgacatc gacaagtacc gccagttgct ggaacgggcg gaggacgccg aggatctcag
    24721 gactctgacg tccatgcgcc gccagaagct caagttcaga cgattggacg acgtactgaa
    24781 agagatcggc agtcgtgcat gagatcctcg tctccagcca ggcagggcga gatcttcgac
    24841 gcgcgcccgc tgtcattctc caacgggtag tccgggccat caaagcgctc tccaacgatc
    24901 cccgtccatc gggcgcgcga aagatcaccg ggtcgaagaa cgattggcgg attcgaatcg
    24961 gggactatcg cgtcctgtac gaactgaacg acactgagcg cacggttcgc gtcatgcgag
    25021 taaggcaccg gcgagaggtt taccggtaaa cgtgtccgtg ctcccgcgac ttcaggccgc
    25081 gcctacgccg ccacgaggtt cttcatgttc cagccgcaga tggtgctgcc ctggagcttg
    25141 tcgcccgcgg ggccggccca gtgagtctcg aggctgatgt aaccgcggta gccgtcggcc
    25201 agcagcgccg caatctgtcc cttccagtcg attccgcagg caccgagcgg cccccactcg
    25261 ggcttgtgcc cctttatata gcagtccttg gcgtggacat gcgcgatccg gttcgccggc
    25321 aacagacgat agccgtccgg gaacggcact tcgccggaaa ccagcgcgtt ggcgggatcc
    25381 cagaccagct tcaagttcgg atgatccaca gccctcagcg ccgccgcggc ctccgcgccc
    25441 gtcgcgaggt tgcaggcgtg ctcgttttcg agcccgatga tgaggtcctc cttggatgcc
    25501 ttctccgcca gttcgccgag cgccgtgaca atccggtcga aacacttcag cggttcgacc
    25561 gtccgccagt acgagaacac gcggatgatc ttcgcccccg tccggtgcgc gatctcgaag
    25621 gcgtgagcag ccagccgggg ttggccctcg taagtgtgct tggaagcgaa gatgtcgtgc
    25681 tggaaacggc tgtcaatctc cggtgagtcg ggcagcacgc acttaagcag cggtgacgca
    25741 attgacatca cctgaaggcc cttgccgtcg agaatctgtt tcgcccggtc gagttcggcg
    25801 tccgtgaggt ccatgatgtt cttgccccac agaacgcgta actccgcgcc ggtcatgccg
    25861 attttcgaca tggcgtccac ggcgacttcc agatcgggcg aaaactcgtc agtgatggcg
    25921 gcgataggca gtttcaggtc catacgtcga ttgtggcata ggaaaacgtg ccggcttttg
    25981 gagtgcgtca gcggatcagt ggacggttaa gttccgtgcg ccagttggcc gggtcggggc
    26041 tcgattccgg gcctgcgacg tagaattccc agaggtccgg acccggcttg tgcccgttgg
    26101 aggcgatcca ggcgccgaac tctccccacg cggcgcccag tccctcgtag ccgccatggt
    26161 agacagtccg tgccaccttc ttggcgggca gttcgcccgg cttcacgcgc cccgccgggg
    26221 aaataggctt agcgacaggc acgccgacct cgaaatcaaa ggtgtccgga tccatcctga
    26281 agtggtacga gaacactggc ccggcggggg caatgccttg ggccgcgacg gtggccatca
    26341 cttcgctgat ggccgggccc atgacattcc tgatctccgc ccgcggaatg atgaggccga
    26401 tgacggcggt gagttgggcg gtgctctgcg tgatctgggg tgtgtcgatc atgagaattc
    26461 agtctcctaa caaaggcgtt agaaacaccg tgtttacgac ccgctccgtc cgggcggcga
    26521 catgattccc tttccttcga cgacagtcag aaactgattc ggcttgatct tcgtgaagcg
    26581 ttctttcgaa ccgccaggcc aggtgacttc gaggaccgtc acgatcgggt ctttgccgag
    26641 accgaacgtc agcgcgaggt cgctctgcga acagtaactc gatccgctgt gaacaatttg
    26701 ccattgcctc ccggaggcgc tttccacccg cagcaccgcc ccgatgccgc tgcggttcgc
    26761 tttcgccccg acggccttca cacacagcca gttgttccgg tttccgccgt cgttgcggaa
    26821 caggtatgcc gggccgtggt tggtgctgat cagcaggtcc aggtcgccgt cacggtcgta
    26881 atcgccgtac gctgcgccgc gcgccacgat cgggcgcttg aacgactgcc ccgcccccgt
    26941 tccgacttcc tcaaaccgcc ctttgccgag attgtggaac aacagaggcg gctgctggaa
    27001 ttggatcttc ggctgcaccc gcccgatctc ctcctcaatg tggccgttgg ccgcgaacag
    27061 gtccggcagg ccgtccaggt cgtagtcgaa gaagaacacg ccgaacgcca ggctcaacag
    27121 gcttgccttg ccgaccgcgg agcgcggggc ttcatcgacg aacagcccgt taccctcgtt
    27181 gtggtacagc ccgagcatct gattcgagaa gttgccgacg agcaggtgcg gccgtcccga
    27241 gcggtcatag tcggcggcgt cgactcccat cgcccc
//
LOCUS       DATIFQ010000118        14871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1569682, whole genome shotgun sequence.
ACCESSION   DATIFQ010000118 DATIFQ010000000
VERSION     DATIFQ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14871
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569682"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(356..3058)
                     /locus_tag="VLE22_05055"
     CDS             complement(356..3058)
                     /locus_tag="VLE22_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSB13805.1"
                     /translation="MIGRTISHYQILEKLGEGGMGVVYKARDIHLDRFVAVKILPPEK
                     VVDPERKRRFVQEAKAASALSHPNIIHVYDIDQADGIDFIAMEFLAGKTLDRLIPRHG
                     MPLTEVLKCAVQIADALAAAHGAGIVHRDLKPANVMVNEPGQVKVLDFGLAKQTVTAE
                     SSEASETWTMGPQTENGTILGTIAYMSPEQAEGKPLDFRSDIFSFGTLLYEMVTGRRA
                     FEADSTAATLVALLHENPILPSEVVEGIPPELDRIIDRCLRKQPEKRFQSTSYLKFAL
                     EDIADDLEAGRPVRAGVKIERSATSRWLRLVTVPLGVTLIAAIWWGWRPGEAPQPAPL
                     TLLTGDAGLSIDPTLSSDGKLVAYASDRTGDGDLDIWVQQRAGGAPIRLTRDPGVERE
                     PMFSPDGNTIAFRSDRDGGGIYVIPGLGGEARLVVKGGRNPRFSPDGSEIAYWVGRGT
                     RGRVQVVPSRGGTPRDLLPESDQGAYPVWSPDGKHVLMRDFREQQYDAWVVPATGGPA
                     MRTGLYDYLREHGFEPWQQQVVWQWLPGNALLFSARLGDSTNLWSIQVSEGSWKVTGE
                     ARRITSGAGVDTSPSVAAGVLAFASLRPSTDIYGAPLEPNRGVLTGDPIRLTERSSPH
                     EMPSVTERGDKVAYRASRFGSLDVWLLDLTRGKDTALTSTQEGEWNPSITWDGSGVTY
                     YFCDPTDCFGYFLRQGEVIPELLCKGCRDPVLSRDGAWILHTDSTVHGLYLQERASGG
                     RTALVRDARYNIWQPGLSPDGRWACFATERERDTCRVHIIPAQGNGPVAESAWITIGA
                     GHWDDKPRWSPDGNLLYFVSDRDGYLCIWAQDLDPATKQPRGEPRAVYHAHGSRRSLA
                     NTEVSTLDISISRDKLVFNMTEVAGSIWATASHGGVAEGVGRAR"
     gene            3392..3712
                     /locus_tag="VLE22_05060"
     CDS             3392..3712
                     /locus_tag="VLE22_05060"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HSB13806.1"
                     /translation="MAKYLYHASYTAEGVRGLLKDGGSSRREAIRKMTESLGGTMEAL
                     YFAFGEDDVYLIVDVPDHVTAAAASLAVAASGAVNIKTTVLLTPEEIDAAAKKTVAYR
                     PPGR"
     gene            3967..4164
                     /locus_tag="VLE22_05065"
     CDS             3967..4164
                     /locus_tag="VLE22_05065"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HSB13807.1"
                     /translation="MQRGLVTPVILICTGGLLLANNVLPDFDLGQWWPVVVIGIGTVL
                     LLDRFCNRQPRIESPSLDRRD"
     gene            complement(4180..4578)
                     /locus_tag="VLE22_05070"
     CDS             complement(4180..4578)
                     /locus_tag="VLE22_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13808.1"
                     /translation="MKAKPAISTLFLAAAVYDGILGMAFLSVPASIFEWFNVTPPNHR
                     GYIEFPALLLLSFAVMYVAIARNPVANRNLIPYGMLLKVSYCAVVFYHWVRGGIPNLW
                     KPWAFADLVFLVLFYAAWRRLAAEAESTAS"
     gene            complement(4526..5905)
                     /locus_tag="VLE22_05075"
     CDS             complement(4526..5905)
                     /locus_tag="VLE22_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB13809.1"
                     /translation="MRIGLIAMSGIRVCDAELLRLGLTLPGFVERSKTIASLPSLGLL
                     TLAGMTPAEHDVRYIEMPEFNASTSIPEDLDLAAISSYSAQIGEAYALARTYRELGVP
                     VVIGGPHVTALPEEAAESADAVVIGEGEACWNEVLEDCAAGRLKQFYGSRESAFDLAH
                     APMPAFDMLDPSKYNRFTVQTSRGCPHQCEFCGSSVLLTGKYKQKPVEKVLAEIDRIL
                     AICEHPFLEFADDNTFVDRSYWKELLRALAERRVRWFAETDLSVSTDDELLSLMRQSG
                     CAQVLIGFESPIEERLRGLELRNDWKRRQWPSYREGIRRIQDHGITVNGCFVLGLDGD
                     DSGVFERVLSFAEETELYEVQVTILTAFPGTPLYSRLKHEGRILADSRWDKCTLFDVN
                     FRPAGMSPEELAEGFRKLVVKLYGEECTNRRRNAFLRRLRAQSKEKEAAHHESQTCDL
                     DAVSGSGSL"
     gene            complement(5902..6285)
                     /locus_tag="VLE22_05080"
     CDS             complement(5902..6285)
                     /locus_tag="VLE22_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13810.1"
                     /translation="MQVEAAYAAGWIGISLGCVAGAVQGLFFHGQNWFGGYNSWTRRM
                     LRLGHISLVGIGLLNLAFALTLNALGITDGALWPLRLLFTGAVTMPLVCYLSAFKSDF
                     RHLFFIPALSVIAAAVLTAWRVLTR"
     gene            6551..8986
                     /locus_tag="VLE22_05085"
     CDS             6551..8986
                     /locus_tag="VLE22_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB13811.1"
                     /translation="MFHDLRQSFRALKREPGFAALAVLTVALGIGANTAIFSIVNGTL
                     LRPLPFAQPDRLVSIRATMPAIAQTYPTLPVSAPLFLEFREHATSFERLSAIHSGSIG
                     LTGAGEPEQLEAARVSANLFDTLGVRPRLGRGFLPHEDQQPGHRVAVITDSLWRRRFN
                     ADPSVIGRTIRLDQDPYTVIGVLPGWFRFPGANLLPIGGQMASSKPEIFRPLAFEPGE
                     LAEFYGCNYGVFARLKPHVSVQRANSELNVITAQALRRRGDSNELRTVAVPMLDSMVG
                     QTRRGLFVLLGAVGAVLLIACVNLANLMLARADRRSHESAIRTALGASRRRLVWSALF
                     ETLLISLLGGILGVAFAAAGLGALIRSAPVDLPRLEEVRIDQVVLLFAFALTTLTGLL
                     FGLAPAWRSASADPQDSLRSGGRTSTSAAGSGRLRACLVAGEVGLSVVLLIAAALLAR
                     SFVRLMRTDPGFHAASALAADVQVPWNKYDAGQRVGFYKRVVDDLSSRPGVNSAALVT
                     MPPLQGEMWIDTFSVPGDTRRNWERPSANIRFASPDYFRTMGIPMISGRTFSENDRPR
                     EVAIVSAKLAEALWPGQDPVGRRVSRHGAEYEVVGVVGDVRAEPHKPAAAILYFPYWM
                     WAPYGAAPRRMVVVVRSEGDPRAVAGAFRAAVRAADPEVPAPAVHAMTEVLEESVAQR
                     RFQMLLISAFGVTALLLAGLGIYGVVSYTVARRRNEMGLRLALGARPGHIYAIVFRQV
                     LTPVAFGLLAGVAGALATGRVLAALLYQVSPRDPLTMTAVASLLIAVTFVACLVPARR
                     ATRQDPLTALRNE"
     gene            complement(9032..10153)
                     /locus_tag="VLE22_05090"
     CDS             complement(9032..10153)
                     /locus_tag="VLE22_05090"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HSB13812.1"
                     /translation="MLTTRWNQAGVVLAIVMATTAWADEVASVRLPSRKQIRAVMRRV
                     NSWQLSHPVMKADDRNWERGTWYTGVVAAWKATGDKSYLDQALAWGKQHNWQVGTEPF
                     GGNRLFCVQTWTELYLLHKRDPAMIEPAIRWLDTPDPRSPAGGRQRWYVEGSRVYVDS
                     LYGASALAMLSRATGKPGYLDIMQAFFDDVSEQLWDEPSGLYYRDERFKGQLSPNGKK
                     VFWSRGNGWVFAGIPRILDYMPAGDPRRARYIEIYRRMASELIQRQHSDGLWRASLDD
                     PQSLPDPESSGTGFFCYGLAWGINQGILDPKEYGDPVIKAFNGLMRSVSADGKVLWGQ
                     PVDAQPNPAARESTHEYVTGTFLLAASEVYRMKRGRGPR"
     gene            10257..10727
                     /locus_tag="VLE22_05095"
     CDS             10257..10727
                     /locus_tag="VLE22_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB13813.1"
                     /translation="MREIADSLTATLEQALPVLEAVREEDASKPALGGGWSRKQVVGH
                     LIDSASNNHQRFVRAGLAGGLEWPGYDQNGCVNIQAYDQAPWAMLLEIWSAMNRLLGH
                     VLRYLPAEAAGAVCRIGNYQPMPLRELAQDYVSHLRHHLSQIGVEIEIDGHGRN"
     gene            complement(10709..10769)
                     /locus_tag="VLE22_05100"
     tRNA            complement(10709..10769)
                     /locus_tag="VLE22_05100"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            11331..12188
                     /locus_tag="VLE22_05105"
     CDS             11331..12188
                     /locus_tag="VLE22_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13814.1"
                     /translation="MLAVCIGALQPSPGWAQAAKSAAAMQPQTSPTVEQPDAQRTREE
                     LSALLQRYPPTLSSVLALDPSLLGSQSYLAPYPALVNFLGAHPEIARNPSFYLGWGPG
                     GRLNQDPATQLQELWRGVLAGLAVFAGFGMAIGLLVWLIRTLVDYRRWSRLAKVQTDV
                     HTKLLDRFTANDDLLAYIQSPAGARFLQSSPITLDAAPRSVGAPLGRILWSVQGGLVL
                     IAGGIGLQVVSGRVADDASQPIHALGILGIALGLGFVISAIISFVISQRLGLIEPASP
                     APRNEPPSA"
     gene            12224..12802
                     /locus_tag="VLE22_05110"
     CDS             12224..12802
                     /locus_tag="VLE22_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSB13815.1"
                     /translation="MFRETTYTDIRRDCSLDDASAGFVMDEDAFRGFYERTARGLWVY
                     LARVTGDRQLADDLLQETFYRFLRAAATHDSEAHRRNSLYRIATNLARDARRRSLTRA
                     PSSLTGNDIERLPSGDEAGATERMTDFTRAMAQLKPRERAMLWLAYGEGISHGEIADL
                     LGLRPASLKPMLFRARRKLAALLGRAPDEGKQ"
     gene            12799..13308
                     /locus_tag="VLE22_05115"
     CDS             12799..13308
                     /locus_tag="VLE22_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13816.1"
                     /translation="MKPVECEFESEALTAVLQSRWPERVDASLRAHVAVCAICSDVVA
                     IAVTIDHARNEMRARAVVPDPGRVWWLAQLRARREAIKAVGRPITVAQVIAFACAVGL
                     LGACFGATSAWFQSALARITTTVAEFRIQALLPSATALLAEHSALALAMAALLFLVPA
                     AVYLAISRD"
     gene            13380..13766
                     /locus_tag="VLE22_05120"
     CDS             13380..13766
                     /locus_tag="VLE22_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSB13817.1"
                     /translation="MVPFRLVPLSGIPIAEQVAFAAKKAIFSRKLRPGDAFPSVRTLA
                     RAMKIHANTAQKVIAQLATEGLLEVLPGIGTVVAQPAAPRSGRARLLAHEVEELAVQA
                     MQIGVSLEELQSAVNECWRSLAGKDE"
     gene            13763..14659
                     /locus_tag="VLE22_05125"
     CDS             13763..14659
                     /locus_tag="VLE22_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB13818.1"
                     /translation="MMIRTENLTKRYGRQEALHGVTLEVPPASVFALVGPNGAGKSTA
                     IKIVMNLVRPSAGRVEILGVDSRRLGAEQLAEIGYVSENQKLPEWMRVDYFLDYSKAL
                     YPSWDDALASELVRELELPLGHRLSALSRGMKVKAALAVSLAYRPRLIILDEPFSGLD
                     VLVREQLIETIVDRTPEATVFLASHDLAEIESFATHVGYLNEGRLEFVEEMAAISARF
                     REIEVTLDAPSGLPSGLPATWLNPEQAGVVVRFTDTSYEARRLHDEVARRFAGVRDIR
                     ARELSFRAIFLALARSRRVQCA"
     gene            14650..>14871
                     /locus_tag="VLE22_05130"
     CDS             14650..>14871
                     /locus_tag="VLE22_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13819.1"
                     /translation="MRLMLHTLKKDARRLWPAAAVTWLMLAALANADRWRADWIPSAM
                     EGWMNLLLSGAWACLAALAVLEEPLVGDRH"
BASE COUNT         2620 a         4829 c         4481 g         2941 t
ORIGIN      
        1 tgcttcctca gcagtaaatt gcacaggcgc tgcttgaagc ggtagatcag ctcgagcacc
       61 ggatgcgggg caaggtacgc cgcgagcttg gcctgctgtt cggggctgcg cttgtggcga
      121 tggcggcgca ttaaccaccg caggcccgcg gtgcttgctg ccggctcgat ccaactcccg
      181 ccagcaggcc aggaagtggt tgatgagccg gatcacgtgg aagcgatcgg ccactccacc
      241 ggcttccaag acttccgtgg ccagggtgtg gcgaaacctg tgaccatgag cgccaggcac
      301 tccggatgct cggaagacgg tgtcccctcc gagcctccga aagggcgttc tggagtcagc
      361 gcgctcgccc cactccctcc gcgacgcccc cgtgcgacgc cgtcgcccag atactgcccg
      421 ccacctccgt catgttgaac acgagcttgt cgcgggaaat ggagatgtcc aacgtcgata
      481 cttcggtgtt ggccagcgat cgccgcgagc cgtgcgcgtg gtacaccgcc cgcggctcgc
      541 cccgtggctg ttttgtggcc gggtcgagat cctgagccca tatgcacaaa tagccgtcgc
      601 ggtccgaaac aaaatacagc aggttcccgt cggggctcca gcgcggtttg tcgtcccagt
      661 gcccggcccc gatcgtgatc cacgcgcttt ccgccaccgg cccgttgcct tgcgccggga
      721 tgatgtggac ccggcacgtg tcccgctccc tctcagttgc gaagcacgcc cagcgcccgt
      781 cgggagacag tccgggctgc caaatgttgt aacgggcgtc gcgtaccagc gcggtccgcc
      841 ctccggatgc tcgctcctga aggtacagcc cgtgaacagt tgagtccgta tggaggatcc
      901 aggctccgtc ccgcgacagc accggatcgc ggcacccttt gcagagtaac tcaggaatga
      961 cttcaccttg ccgcagaaag taaccaaagc aatccgttgg atcgcagaaa taataagtca
     1021 cgccggagcc gtcccaggtg atcgatgggt tccactcccc ctcctgcgtg gaagttaggg
     1081 ccgtgtcttt tcctctggtg aggtccagca gccaaacgtc caggctcccg aaacggctcg
     1141 cccggtatgc caccttgtcg ccgcgctccg tcaccgaggg catttcatgc ggcgaagagc
     1201 gctcggttag ccgaataggg tcgcctgtga gcacgccccg gttcggttcg agcggcgctc
     1261 cgtaaatgtc ggtgctcggc ctcaagctgg cgaacgccaa cacgccggcc gccacggaag
     1321 gcgacgtgtc cactcccgca cccgaggtga tcctcctggc ctcacccgtg accttccacg
     1381 acccctccga gacctggatg gaccataggt tcgtactgtc gccgagccgc gctgaaaaca
     1441 gcagagcgtt gccgggtagc cactgccaga ccacttgctg ctgccatggc tcgaatccgt
     1501 gctccctcag gtagtcgtac aacccggtcc gcattgccgg gccgcccgtg gccggaacca
     1561 cccacgcgtc gtattgctgc tcacggaaat cccgcatcag aacatgcttg ccgtccggag
     1621 accaaacagg ataggcccct tggtccgatt ccgggagcag atcgcgcggt gtcccgccac
     1681 gggatggaac cacttgcaca cgaccgcgcg ttccgcgccc cacccagtag gcgatttcag
     1741 atccgtcggg agagaaccgc ggattgcggc ctcccttcac caccagcctt gcctccccgc
     1801 ccagacccgg aatgacgtag atcccgcctc cgtcgcgatc ggaccggaag gcgattgtgt
     1861 ttccgtcggg cgagaacatt ggctcgcgtt cgaccccggg gtcccgcgtc agccgaatcg
     1921 gcgcgccgcc ggcccgttgc tgtacccaga tgtccagatc gccgtctccc gtgcggtcgg
     1981 atgcgtacgc caccagcttg ccgtccgacg acaacgtcgg atcgatcgac aggccggcgt
     2041 cgcccgtgag cagcgtaagc ggtgcgggtt gcggcgcttc tcccggccgc cacccccacc
     2101 agattgcggc aatcagtgtc accccaagag gcacggtgac cagccgcagc cagcggcttg
     2161 tcgccgaacg ctcgatcttg actccagccc tcactggccg gcctgcttcg aggtcatcag
     2221 cgatgtcctc cagggcaaac ttcagatacg aagtgctctg gaatcgcttc tccggctgct
     2281 tgcgcaggca ccggtcaatg atccgatcca gctccggcgg tatgccttcc accacctcgc
     2341 tcggcagtat cggattctcg tgaagcagcg ccaccagcgt tgccgccgtg gaatcggctt
     2401 caaaagctcg acgacccgtc accatctcgt acaacagcgt tccaaagctg aagatgtctg
     2461 accggaagtc cagtggcttg ccctcggctt gctccggcga catgtaggcg atggtcccga
     2521 ggatggttcc gttctccgtc tgcggcccca ttgtccaggt ctcgctcgcc tcgctgctct
     2581 ccgctgttac ggtctgcttc gcgagcccga aatccagcac cttcacctgc ccaggctcat
     2641 tcaccatcac gttggccggc ttcaagtcgc ggtgcacgat accggctccg tgagcggccg
     2701 ccaacgcatc ggcgatctgc acggcgcact tgagcacttc ggtcagcggc atcccgtgcc
     2761 ggggaatcag ccgatcgagc gttttgccgg ccaggaattc catcgcgatg aagtcgatac
     2821 cgtcggcttg gtcaatgtcg tagacgtgga tgatattggg atgactgagt gcggaggcgg
     2881 ctttcgcctc ctgtacgaac cggcgcttgc gctcgggatc gacgaccttc tccggtggca
     2941 ggatcttcac agcgacgaaa cggtcgaggt ggatgtcccg agccttataa accacaccca
     3001 tcccgccctc gccgagcttc tcgaggatct ggtagtgcga gatggtgcgg ccgatcatgg
     3061 cccgccctcc tttcggacaa catgaggtaa ccaatcagga tcctctttgg tgccgtccgg
     3121 ttagccggag tatatcacca ccatgctgtt tcttgacgtg cggacgcggt ccttttttcc
     3181 tcaataatgc tgctgtctaa ccctgacagg ggtatccagg caggcctaag attgcctctg
     3241 tgctgatcaa aacgtttcct tccaaggatg ttttgtcagt cagacactac agaatgcccc
     3301 caataatgtc atcagttccg atggaggtcc gccagctcgc tggaccactg gacactgtgg
     3361 tatcctgctc ttcagaggga gggactttca gatggccaag tatctctacc atgcttccta
     3421 cacggctgag ggcgtgagag gactgctcaa ggacggggga tccagcagac gcgaggcaat
     3481 taggaagatg accgagtctc tcggcggaac catggaggcc ctctactttg ccttcggtga
     3541 ggacgacgtg tacctgattg tggacgttcc cgaccatgtc acggccgccg ctgcatcgct
     3601 ggcggtcgcc gccagcggcg cagtgaacat caaaacgacg gtgcttctca caccggaaga
     3661 aatagacgct gccgcgaaga agactgtcgc ctatcgaccg cctgggcgat agcgggcaca
     3721 gccaccaaat tgtctgaggc atcggactac gcccgtgagg cggagcggtt cttacaggct
     3781 cattcttccg aggtcctgac tgttagtgcc ggatagcgcc cgaaagcgcc cgctttcgtc
     3841 gcctatcggg ctggagacgc cgtggtcttc ttcgattgcg tctgccgata atccttccga
     3901 actttggcca gcgggatctc gtttcttcta atgggtggga tcaatacccc agtgggaggt
     3961 cctaagatgc agcgcggtct tgtgactccg gtgatcctga tttgcacggg cgggttgttg
     4021 ctcgcgaaca atgtcctgcc cgatttcgat ctcggccagt ggtggccggt agtagtgatc
     4081 gggatcggaa cagtcttgct cttggaccgc ttctgcaatc gtcagcctcg gatagaaagc
     4141 ccttcgcttg accgccgaga ctgagagtaa agagacctgc tacgacgcag tgctctccgc
     4201 ttccgctgcg agccggcgcc acgcggcata gaacagaacc aggaacacca ggtcggcgaa
     4261 agcccacggc ttccataggt tcgggatgcc gccgcggacc cagtggtaga acaccacggc
     4321 acagtaggag actttcaaca gcatgccgta ggggatgagg ttgcggttcg ccaccggatt
     4381 gcgggcgatg gcgacgtaca tcaccgcgaa cgatagcagc agcaaggccg ggaattcaat
     4441 gtagccgcga tggttcggcg gagtcacatt gaaccactcg aagatactgg ccgggactga
     4501 caggaacgcc attcccaaaa ttccgtcata aactgccgct gccagaaaca gcgtcgagat
     4561 cgcaggtttg gctttcatgg tgtgccgcct ccttttcttt gctctgcgcc ctgagccgcc
     4621 tcagaaacgc atttctgcgc cggtttgtgc attcctcgcc gtatagcttc accaccagtt
     4681 tcctgaagcc ttccgccagc tcttcgggac tcatgcccgc gggcctgaag ttcacatcga
     4741 agagcgtaca cttgtcccac cggctgtctg caaggatccg gccttcgtgc ttgagccggg
     4801 agtaaagcgg agtgcccggg aaggcggtca ggatggtgac ctgcacttca tacaactccg
     4861 tctcctcggc gaaagagagg acccgctcga agactccgga gtcgtcgccg tcgagcccca
     4921 acacgaagca gccgttgacg gtgatcccgt gatcctggat cctgcggatg ccttcccggt
     4981 aactgggcca ctgcctgcgc ttccagtcat tgcgcaactc caggccgcgc aagcgttctt
     5041 cgatggggct ttcgaacccg atcagaacct gcgcgcagcc gctctggcgc atgaggctca
     5101 acagttcgtc gtcggtgctg acggaaaggt cggtctcggc aaaccagcgc acacgccgct
     5161 ccgccagcgc ccgaagcaat tccttccaat aactccggtc cacgaaggtg ttgtcgtcgg
     5221 caaactccag gaagggatgc tcacagatgg cgagtatcct gtcgatttcg gccagcacct
     5281 tttcgaccgg cttctgcttg tacttgccgg taagcaggac cgagctgcca cagaactcgc
     5341 attgatgcgg gcacccgcgg ctggtctgca cggtgaagcg gttgtacttg gaggggtcca
     5401 gcatgtcgaa agctggcatg ggcgcgtgtg ccaggtcgaa tgcgctttca cgcgacccgt
     5461 agaactgttt cagccggccc gctgcgcagt cctccaatac ctcgttccag cacgcctccc
     5521 cttcgccgat gacgaccgcg tccgccgact cggctgcctc ttccggcagc gctgtcacgt
     5581 gcggcccacc gatgacgacg ggaactccga gttctctgta cgttctcgcc aacgcgtagg
     5641 cctcaccgat ttgagcgctg taactggaga ttgcggccag atccaggtcc tcaggaatgc
     5701 tggtggaagc gttgaactcc ggcatttcga tgtagcggac atcgtgttcc gcgggagtca
     5761 tgccggccag tgtaagaagc cccaggctcg gcagcgaggc gatggtcttg ctgcgttcga
     5821 cgaagcccgg caaagtcaaa ccgagccgca gcagttccgc atcgcaaacg cggatgccgc
     5881 tcatggcgat caagccgatt ctcatcgcgt aagaactctc caggcggtca ggacggccgc
     5941 tgcgatcacg ctcagcgcgg ggatgaaaaa caggtgacgg aaatcgctct tgaatgcgga
     6001 gagatagcac accagcggca tcgtgacggc gccggtgaac agcagccgca aaggccacaa
     6061 tgcgccgtcg gtgatgccca gggcgttcag cgtcaacgcg aacgccaggt tgagaagtcc
     6121 gatcccaacc agtgagatgt gccccaaccg cagcatccgc ctggtccatg aattgtagcc
     6181 gccgaaccaa ttctggccgt ggaagaatag cccttgaacg gccccggcaa cgcacccgag
     6241 cgagattccg atccaacctg cggcataggc ggcttcgacc tgcatgttag gctctaaaac
     6301 aattgaactg gtcttccggg ccgcgcaatt tcggatggcg gccacttaaa cgattatcgc
     6361 cccaagtcac ttagaagcaa agagaaatcg gcccttctca aagaccagag aggtcgattg
     6421 ttgcgggtga agcgcggcat gatcgccaag cttttcggtg cgctccggtt ccacatcacg
     6481 accctggtgg aggatcaggc gatcatcgga acattccttc ccaactctgc gtatgttccc
     6541 gcatatgtcc atgttccacg atcttcggca gtccttccgt gcgctgaagc gcgaacccgg
     6601 tttcgccgct ctcgccgtcc tgactgtcgc actcgggatt ggggctaata cagcaatctt
     6661 ctccatcgtc aacggcacgc tgctccgtcc gctccccttt gcccagcccg atcgcctcgt
     6721 cagcatccgt gcaaccatgc ccgccatcgc tcagacctat cccaccctcc cggtgagcgc
     6781 ccccctgttt ctggaattcc gcgagcacgc gacttccttc gagcgcctct ccgccattca
     6841 tagcggcagt attggcctta ccggcgccgg agagcccgag caactcgagg ccgcgcgcgt
     6901 gtccgcgaat ctgtttgaca ctcttggcgt aaggccccgc ctgggccgcg gcttccttcc
     6961 ccacgaggac cagcagcccg gccaccgcgt tgcggtcatt acggactcgc tctggcgccg
     7021 gcgcttcaat gccgaccctt ctgtcatcgg ccgcaccatt cgcctcgatc aggatccgta
     7081 cactgtgatc ggcgtcctgc ccggctggtt ccggtttcct ggcgctaacc tgctccccat
     7141 tggcggccag atggcatctt cgaaacccga gatcttccgg ccgctggcct tcgagccagg
     7201 cgagctggcc gagttctatg gctgcaacta cggcgtgttc gcccgcctca agcctcatgt
     7261 ctcggtgcag agagccaact ccgagctgaa cgtcattacc gcgcaagcgt tacgccgaag
     7321 gggcgactcc aacgaactac gcaccgttgc cgtccccatg ctcgattcca tggtcggcca
     7381 aacgcggcgt ggcctcttcg ttcttctggg cgctgtcggc gccgttctgc tcatcgcttg
     7441 cgtgaacctc gccaatctca tgctggcgcg ggccgatcgc cgttctcacg aatccgccat
     7501 acgtaccgca ctcggtgcga gccggaggcg cctcgtatgg tccgccctct tcgaaaccct
     7561 gctcatctct ctgttgggcg ggattctggg tgtcgccttc gctgccgccg gcttgggtgc
     7621 gctcattcgg agcgctcccg tcgatctacc gcggctcgag gaggttcgca tcgaccaggt
     7681 cgtcctgctc ttcgctttcg ccctcacgac ccttacgggt ctgctcttcg ggctcgcccc
     7741 cgcgtggcgc tccgccagcg ccgacccgca ggactcgctc cggtccggtg gccgcacgtc
     7801 aacgagcgcg gccgggagcg gccgtctgcg cgcctgcctg gtggctggcg aggtcggcct
     7861 cagcgtcgtg ctcctgatcg ccgcggccct gctggcccgc agcttcgtcc gcctcatgcg
     7921 caccgatcct ggttttcacg ccgcctcggc cctggccgcc gatgtccagg tgccctggaa
     7981 caagtacgac gccggccagc gcgtcggctt ctataagcgc gtggttgacg atctctcctc
     8041 tcgcccgggc gtgaactcgg cggcgctcgt gacaatgccc ccgctgcaag gtgagatgtg
     8101 gatcgatact ttctccgtgc ccggtgatac acggcgcaac tgggaacgac cttccgccaa
     8161 catacgcttc gccagcccgg actatttccg cacgatgggg atccccatga tttccggccg
     8221 caccttcagc gagaacgacc gcccgcgcga ggtcgccatc gtttcagcaa agcttgctga
     8281 agcgctctgg cccggtcagg atccggttgg acggcgcgtc tcaaggcacg gcgccgagta
     8341 tgaggtcgtc ggcgtcgtgg gcgatgtacg cgccgagccg cacaaaccgg cggccgccat
     8401 cctctacttt ccgtattgga tgtgggcgcc ctacggggcc gcgccgcggc gcatggtggt
     8461 tgtggtacgc agtgaagggg atccgcgcgc cgttgccggg gctttccggg cggccgttcg
     8521 cgccgccgat ccggaggtcc ccgcgcccgc cgtccatgcc atgaccgaag tgctggagga
     8581 atccgtggcc cagcgccgtt tccagatgct gctcatctcc gctttcggtg taacggcgct
     8641 cctccttgcg ggattaggca tctacggtgt ggtctcatac acggtggccc gccgccgcaa
     8701 cgagatgggt ctacgtctgg ccctcggcgc gcgcccaggg cacatctacg ccatcgtctt
     8761 ccgccaggtt ctcacgcccg tggcgttcgg cctgctggcc ggcgtcgcgg gagcgctggc
     8821 taccgggcgt gtgctcgcgg ctttactgta ccaggtaagt ccccgtgacc cgctgaccat
     8881 gacggcggtg gccagtctac tcatcgcagt cactttcgta gcctgtctcg tgcccgctcg
     8941 ccgcgccacg cgccaggacc cgctcacggc tctgcggaac gagtaagatg ctcgtggttt
     9001 tggctatcgc ctggtgagga ctgtggccct atcaccgcgg tccgcgcccg cgcttcatcc
     9061 tgtaaacctc gcttgcagcc agcaggaagg ttcccgtaac gtattcgtgc gtgctctccc
     9121 gagcagccgg gttcggttgc gcatccaccg gttgccccca caacaccttc ccgtccgcgc
     9181 ttacggaccg catcaggccg ttgaatgcct taatgaccgg gtctccatat tccttagggt
     9241 cgaggattcc ctgattgatg ccccatgcga gtccgtagca gaagaatccc gtgccgctgc
     9301 tctcgggatc aggcaaggac tgtggatcat ccaggctggc gcgccacaaa ccatcactgt
     9361 gctggcgctg gatcagttcg gatgccatac ggcggtagat ctcgatgtat cgggcacgcc
     9421 gtgggtctcc ggccggcatg tagtcgagga tacgaggaat gccggcgaat acccacccgt
     9481 taccgcgcga ccagaaaacc ttctttccat ttggagagag ttgccctttg aaccgttcgt
     9541 cgcggtagta gaggccggac ggttcgtccc aaagctgctc cgatacgtcg tcgaaaaatg
     9601 cttgcatgat gtctaggtag ccgggctttc cggtagcgcg ggacagcatt gccagagccg
     9661 acgcgccata gagtgagtcc acatagacac ggctgccttc aacgtaccac cgctgccgtc
     9721 caccggcagg cgagcgcggg tccggtgtgt cgagccagcg aatggcgggc tcaatcatcg
     9781 cgggatccct tttgtgcaac agatacaact cagtccacgt ctggacgcag aagagccggt
     9841 tgccgccgaa cggctccgtg ccgacctgcc agttgtgctg cttaccccag gccagcgcct
     9901 ggtcgaggta gctcttatcc ccggttgcct tccatgcggc gaccactccg gtgtaccatg
     9961 tgccgcgttc ccagttccga tcgtctgctt tcatcacggg gtgcgacaat tgccaactgt
    10021 tgacgcggcg catcacagcg cggatctgct tgcgggacgg aaggcggact gaggcaactt
    10081 cgtctgccca cgccgtggtt gccataacaa tcgccagcac cacgcctgcc tgattccaac
    10141 gggttgtcaa cacatccgta gtgtatgtct tctccgagtc ccggctgggg cgccgggtgc
    10201 gagccgcatg gaacaacagt tagaatcttg ataaaacgct gtgttgggaa cactccatgc
    10261 gtgagatagc tgacagtttg acggccacgc tcgagcaggc cttgccggtt cttgaggcag
    10321 tccgcgaaga agatgcgtcg aaaccggcgc ttggaggcgg ctggtccagg aaacaggtcg
    10381 ttgggcacct gatcgactcg gcgtccaaca atcaccagcg tttcgtgcgg gcaggtctcg
    10441 cgggcgggct cgaatggccg ggctacgatc aaaacggctg tgtcaatatt caggcctatg
    10501 atcaggcgcc atgggcaatg ctgctggaga tctggtccgc gatgaaccgg ctgctcggcc
    10561 atgtattgcg gtatttgccg gcggaagcgg cgggcgcagt ctgccgcatc gggaattatc
    10621 aaccgatgcc gcttcgggag ctggcgcaag actacgtgtc ccacctgcgg caccatttga
    10681 gccaaattgg tgtcgagatt gaaattgatg gtcatgggcg gaattgaacc gccgaggcag
    10741 ccttttcagg gctgcttacc gactgcgtta agtggttcag aatcagtgga agtccatggc
    10801 cgaacgagac ttatgcgaaa cgactcttag cactgtttgg gatcatctag gctgtttccg
    10861 cctcttcgat attgcccctt gttcccgcgc atcccgccgt attcctaggg cccaaacgtt
    10921 cggaggtgct catgaaagcg gcactgttcc tccgggtcag cacccgagac ggcacccagc
    10981 gcaccgacaa ccagcggctc caattgcgcc aactctgcac atcgagagtg tggcgcccac
    11041 atcactgtgc gagcggaacc tgaaccgagc accgagtact tgtgcaagtt ccgcgtagtc
    11101 gaccatgcgt ccggaacctt actccgacgc ctccccataa cgccgtttcg gggccgcaaa
    11161 acggggcagc tttggtctga ccgtggtctt ctctccctcc tcgaacggcg gaatactttt
    11221 ttcaatttgg agatcaagtg ttaacctgct caccctgact gcgtaactat atggcagaga
    11281 ggagtgcgat gatgacgcgg gcgcgaatca gaccagtttt gggagcggga atgctggcgg
    11341 tatgcatcgg tgcgctgcag ccgtcgcccg gctgggcgca ggcggcaaag agcgctgcgg
    11401 ctatgcaacc tcagacgagc ccgactgtag agcaaccgga cgcgcagcgc accagagagg
    11461 agctgtcggc ccttttgcag cgctatccgc ccacattgag cagcgtcctg gcgctcgacc
    11521 cgagtctcct cggcagtcag tcctatcttg cgccgtaccc cgcccttgtg aactttctgg
    11581 gcgcgcatcc cgaaattgcg cgcaatccgt cgttctacct cggctggggt ccgggtggcc
    11641 gcttgaatca ggaccccgcc acccagctcc aggagttgtg gaggggcgta ttggccggct
    11701 tggccgtatt cgccgggttc ggcatggcga tcggtctgct cgtctggctg attcggacgc
    11761 tagttgacta cagaaggtgg agccggctgg ccaaagtgca aaccgacgtc catacgaagc
    11821 tcctcgaccg gttcacggca aacgacgatc tgctcgccta catccagtca ccggccggcg
    11881 cgagattcct gcagtcgtcg ccgatcacgc ttgacgccgc tccacggagc gtgggtgcgc
    11941 ctcttgggcg cattctctgg tcggtgcagg ggggcctggt gctcatcgct ggcggcatcg
    12001 gcctgcaggt tgtgagcgga cgcgtcgccg atgacgcgtc tcagccgatc catgctctag
    12061 gcattctcgg catcgccctc ggtctggggt tcgtgatctc ggcgataatc tcatttgtga
    12121 tctcacagag gcttgggttg attgaaccgg catctccggc gcctcggaat gagccgccca
    12181 gcgcctaggg agcccaaact ccgggaagaa acgacgctct cccatgtttc gcgagacgac
    12241 gtacaccgat atcaggcgcg actgctctct ggatgatgcc tctgccggct tcgtgatgga
    12301 cgaggacgca ttccgcgggt tctatgagcg caccgcgcgc ggactatggg tgtacctcgc
    12361 ccgggttacc ggtgaccgtc aactcgcgga cgacttgctt caggaaacgt tctatcgctt
    12421 cctccgcgca gcggcgacgc acgacagcga ggcgcaccgc cgcaactctc tgtatcgcat
    12481 tgccacgaat ctggcgcggg atgcacgacg gcgaagtctg acgcgcgcgc cctccagcct
    12541 gaccggcaac gacatcgagc gccttccctc cggggatgaa gccggcgcaa ctgagcgcat
    12601 gaccgatttc acgcgcgcca tggcgcaact caagccgcgt gaaagggcca tgttatggct
    12661 ggcgtatggc gagggcattt cccacggcga gatcgcggac ttgctcggcc tgcgtccggc
    12721 cagcttgaag cccatgttat ttcgcgcgcg acgaaagctg gccgcgttgt tgggccgtgc
    12781 gccggacgag ggtaaacaat gaagcccgtt gagtgcgaat tcgagtcaga ggccctgacc
    12841 gccgtcctcc agtcccgctg gcccgagcgg gtggatgctt cactgcgcgc tcatgttgcg
    12901 gtatgcgcca tctgctccga tgtcgtggcc atcgccgtta ccattgacca cgcccggaat
    12961 gaaatgcggg cccgtgcggt ggttcccgat ccgggccgtg tgtggtggct cgcccaactg
    13021 cgcgctcgcc gcgaagctat caaagccgtc gggcgtccga ttaccgtcgc tcaggtgatc
    13081 gcgttcgctt gcgcggtggg cctgctgggg gcgtgcttcg gcgccacgtc ggcgtggttt
    13141 caatcggctc tggcgaggat tacgacgacc gtggcggagt tcaggattca ggcgctgctt
    13201 ccttccgcga cagcgctgct cgctgaacac agcgcgttgg ccctcgcaat ggctgccctg
    13261 ctcttcctgg ttcctgccgc cgtttatctc gcgatctcaa gggactgacg acgtgagcac
    13321 agccgtcctc cgctcaaaat tccccttcac accactgtaa catgtgatat agtacacgta
    13381 tggtcccttt ccggctggtt ccgctatccg gcataccgat agccgagcag gttgcatttg
    13441 ccgccaagaa ggccatattc agccgtaagc tgcgcccggg cgacgccttt ccctcggtcc
    13501 gcacgctggc ccgcgcgatg aagattcacg ctaacaccgc gcagaaggtg atcgcacaac
    13561 tcgccacgga gggactgctt gaggtgctgc cgggaatcgg aaccgtcgtg gcgcagccgg
    13621 cagcgccgcg tagcggccgc gcccggctgc tggcccacga agtggaagaa ctcgccgtgc
    13681 aggccatgca gatcggggtc tcgctggagg aactgcagtc cgccgtcaac gagtgctggc
    13741 gcagtctggc cgggaaggat gaatgatgat tcgcaccgag aaccttacca aacggtatgg
    13801 ccgccaagaa gcgctccacg gtgtcacgct cgaagtgccg cctgccagcg tcttcgcgct
    13861 cgtcgggccg aacggcgccg gaaagagcac cgcgatcaaa atcgtcatga acctcgtccg
    13921 gccgagcgcc ggccgcgtcg agattcttgg tgtagattcg cggcggctcg gagccgagca
    13981 actcgcagag atcgggtacg tctcggaaaa ccagaagctg cccgagtgga tgcgggtcga
    14041 ttatttcctc gactattcga aagcactcta cccctcctgg gacgacgcct tggcctccga
    14101 attggtgcgc gagctcgagc tgcccctggg ccaccgtttg agcgcgctct cgcgcgggat
    14161 gaaggtgaag gccgctctgg cggtatcgct tgcctaccgc ccgcgcctga tcatcctcga
    14221 cgagccgttc agcggcctag acgtgctggt gcgggagcaa ctgatcgaga ccatcgtcga
    14281 tcgaactcca gaggccactg tctttctcgc ctcgcacgac ctcgccgaga tcgagagctt
    14341 cgctactcac gtgggctacc tcaacgaggg ccggctcgaa tttgttgagg agatggcggc
    14401 tatctccgcc cgcttccgcg agatcgaagt cacgctggac gcgccgtccg gattgcccag
    14461 cggacttccc gcgacgtggc tcaacccgga gcaggccggc gtcgtcgtgc gcttcaccga
    14521 cactagctac gaagcccgcc gtttacacga cgaggtggcg cgccggttcg ccggtgtacg
    14581 cgacattagg gcgcgagagt taagtttccg cgccatcttc ctggcgctgg caaggtcgag
    14641 gagggtgcaa tgcgcctgat gctacacact ttgaagaagg acgcccggcg tttgtggccc
    14701 gcggcagcgg tgacgtggct catgctcgca gcgctggcca atgccgaccg gtggcgcgca
    14761 gactggattc catccgccat ggaaggctgg atgaatctgc tgctttccgg agcgtgggcc
    14821 tgcctggcgg cattggccgt gctcgaagag cccctggtgg gcgatcgcca t
//
LOCUS       DATIFQ010000119         4377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2528432, whole genome shotgun sequence.
ACCESSION   DATIFQ010000119 DATIFQ010000000
VERSION     DATIFQ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2528432"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1170)
                     /locus_tag="VLE22_05135"
     CDS             complement(280..1170)
                     /locus_tag="VLE22_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HSB13820.1"
                     /translation="MNFSLGCSLVYRVKSPTPLVFNFEAARFNAQTVREERLQLIPKT
                     SIERWTMPESGNRYLRVLAEPGELHVRYEAEIELSPRLEDPDVVTEISAGQLPFEVLT
                     HLYPSRYCQSDKLTRFAGRTFRDLPPGYHRVNGVCNWIRDNVDYVLGSSDLLTSAFDT
                     VTQRVGVCRDFAHLGMALCRALGIPARYVSAYAWRLDPPDFHAVFEAFLNGPSGAGWY
                     LFDPTRMSAPDALVRIGIGRDAAEVAFCTAYEEVESDAPQVWISGDGDATEPITTSAV
                     RTETQPASRSATPTTSDARP"
     gene            1299..2615
                     /locus_tag="VLE22_05140"
     CDS             1299..2615
                     /locus_tag="VLE22_05140"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSB13821.1"
                     /translation="MATRATQPGRPPHVVVIGAGFGGLATVRGLCNTPVRVTWIDRKN
                     YHLFQPLLYQVATAALSPADIAAPARGIARTWRNVETVLAEVTGIDAEARVVKTTRQT
                     YSYEYLVMATGAETSYFGNEKWARFADGLKTLEEAVAIRRKVLLSLELAETAQSEEER
                     RRLLTFVLIGAGPTGVEMAGAIADLAKHMLARDFRHIESGTICVILLEAMDQVLPGYP
                     RDLATFAEKKLKEMGVDVRTGTKVEDIDEEGVVAGGTRIASRTIIWSAGVKATPIADW
                     LGTEADKKGLVKVRPDLSLPSRPEVFVIGDAALALDEEGHPLPGLAAVAKQQGAYVAR
                     CLLDRIKGREPLSPFCYHDWGTLATMGKASAVANFGRLRLRGFVAWVVWVLVHIWYLI
                     GFRNRVRVLLNWVWLYATFSPGARLITGDFNWSELGVRESSEAKAR"
     gene            2707..2901
                     /locus_tag="VLE22_05145"
     CDS             2707..2901
                     /locus_tag="VLE22_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13822.1"
                     /translation="MQRYTVGRNIKGKSDPVTVEAEDALAAAMQAKQQAPDAVITYVR
                     KRNERGDQRHPHHEVANGEG"
     gene            3272..3589
                     /locus_tag="VLE22_05150"
     CDS             3272..3589
                     /locus_tag="VLE22_05150"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HSB13823.1"
                     /translation="MATFIMLTRLAYGAIRSPEQLEKLEKEVMERIEEGLAGGQVKWL
                     ANYAVLGPYDYLDIFEAPDIDAAMKVSAIVRTLGHAHTEVWAATEWKRFKEIVRELPS
                     GGD"
     gene            3849..4238
                     /locus_tag="VLE22_05155"
     CDS             3849..4238
                     /locus_tag="VLE22_05155"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HSB13824.1"
                     /translation="MTPTIPPPEPDSLSSSLKRNIKALEDRRRQQAAAATCEERIAEA
                     ITRFTGSMRFVYLHVVLFGAWFLVNLGVVPGVPRFDPSFVVLAMVASVEAIFLSTFVL
                     ISQNRMADAADKRADLDLHISRSPSTN"
BASE COUNT          881 a         1320 c         1366 g          810 t
ORIGIN      
        1 gggaggggga atttcaactt atagtacttt ttcgccgcgt aagacacaag aatttgcgta
       61 caaggccacc agcgtcgcgc ccctgccggc cggctcgatg gcaaccgccg gcggcaggta
      121 tgcggcgtta tggaaatggc ccgccgccgc tatcctggac cccccgtagc tcgcgaacaa
      181 tcacccgctc gctgaggtca gcgatcagtt gagtcgtcgt tacagtcacc aaggacatta
      241 gcctcacctt aaggctgccc gatgagatcg tggacgtcct caaggacgag catccgacgt
      301 tgtcggcgtg gcggatcggc tggccggctg tgtctcggtt cttacagcgc tcgtggtgat
      361 tggctctgtc gcgtcaccgt caccgctgat ccagacctgc ggcgcgtccg actcgacctc
      421 ctcatacgca gtgcagaatg caacctccgc agcgtcccgc ccaatgccga tgcgcaccag
      481 agcgtccggt gccgacattc gcgtggggtc gaacaggtac cagccggcac cggagggccc
      541 gttcaggaac gcctcgaaga ctgcgtggaa gtcgggcggg tccaagcgcc acgcgtacgc
      601 gctgacgtag cgggccggaa tgcccaaggc acggcagagc gccatgccca ggtgggcgaa
      661 gtctcggcat acaccgacgc gttgcgttac tgtatcaaag gccgaggtca acaggtcgga
      721 cgaaccgagg acataatcga cgttgtcgcg aatccagttg cagacaccgt tgacgcgatg
      781 atagccgggc ggcagatcgc ggaacgtgcg gccggcaaac cgcgtcagct tatcggactg
      841 gcagtagcgg cttggataga ggtgcgtgag cacctcgaaa gggagctgtc cggcggagat
      901 ctcggtgacc acatccgggt cttccaagcg cggcgacagc tctatctctg cctcgtaacg
      961 gacgtggagt tcgcccggtt ctgcgaggac acgcaggtag cggttgccac tttccggcat
     1021 cgtccagcgc tcaatcgacg tcttgggtat cagttgcagc cgttcttccc ggaccgtctg
     1081 cgcgttgaac cgcgcagctt cgaagttgaa gacgagcgga gtgggtgatt tgacgcggta
     1141 gacaagcgag caacccagcg aaaagttcat ggcgatatcc gaccgtgagc aaccggacta
     1201 agacccaatc cggaaacctg acggccacgc acgttcagca tggagtggct tttgaaggga
     1261 aaagccgccg tgcgacctgc atctagcggg agggccccat ggcgacacgc gccacgcaac
     1321 cgggcaggcc tccgcacgtc gtcgtcatcg gcgccggctt cggtgggctc gcgacggtgc
     1381 gcggcctttg caacacgccg gttcgcgtca cctggatcga tcgcaagaat taccatctgt
     1441 tccagcccct gctgtatcag gtcgcaaccg cggccttgtc gccggccgat atcgcagcac
     1501 ccgcgcgcgg cattgcgcga acttggcgga atgtcgagac cgtgctcgcc gaggtcacag
     1561 ggatcgatgc cgaagcccgc gtcgtcaaga ccacgcggca gacttactcc tatgagtacc
     1621 tcgtcatggc cactggtgcg gagacaagct atttcggcaa cgaaaagtgg gcacggttcg
     1681 ccgacgggct taagacgctg gaagaggcgg tggcgatccg ccgcaaagtt ctcctctctc
     1741 tggagctcgc cgaaaccgcg cagtcggagg aggaacgccg acgcctcctg acgttcgtct
     1801 tgatcggggc aggtccgacg ggcgtcgaga tggcaggtgc cattgccgac ctcgctaagc
     1861 acatgctggc gcgcgacttt cgccacatcg agtccgggac catctgcgtc atcctgctcg
     1921 aggcgatgga tcaggtcctg ccgggctatc cgcgcgatct tgcgaccttc gccgagaaaa
     1981 agctcaagga aatgggcgta gatgtccgca cgggcaccaa ggtcgaagac attgatgaag
     2041 aaggtgtcgt cgccggcgga acccggatcg ccagcagaac gatcatttgg agcgccgggg
     2101 tcaaggccac tcccatcgcc gactggctcg gcaccgaggc cgacaagaaa gggctcgtca
     2161 aggtgcgacc ggacctttcg ctcccgagcc gcccggaggt cttcgtcatc ggcgatgccg
     2221 cgcttgcgct tgatgaggaa ggccatccgc tgccgggatt ggccgccgtc gccaagcagc
     2281 agggcgctta cgttgcccga tgtctcctcg atcgcatcaa gggccgcgag cccttgtcac
     2341 ctttttgcta tcacgattgg ggaaccttgg cgaccatggg caaggcctcg gcggtggcca
     2401 atttcggccg ccttcgcttg cgcgggtttg tcgcatgggt tgtttgggtc ctggtgcaca
     2461 tctggtatct catcggtttc cgcaatcgcg ttcgcgtgct gctcaactgg gtatggctat
     2521 acgccacgtt ctcgccaggc gcgcgcctca tcaccggcga tttcaattgg tcggagttag
     2581 gcgtccggga aagctcggaa gcgaaggcgc gatgacggag gtcctgcgtg aggacgtgtg
     2641 ctcgcgttcg gggattaaca gcgggcggcc gccgccgcgt ccatgagggc cacacagagg
     2701 caacaaatgc aacggtatac agtcggacgg aatatcaagg gaaaaagcga cccggttacg
     2761 gtcgaggcgg aagacgcact tgcggcggcc atgcaggcga agcagcaagc tccggatgcg
     2821 gttatcacct acgtccgtaa gcgcaacgaa cggggcgacc agcgccaccc acaccatgaa
     2881 gtggccaacg gcgagggtta atcggggaga ttacgaccgt gcggcgcaag agatcgatta
     2941 cgacgttttc gtgacctctc aagaatccac gtgagaaccc atcgcgaatg gctcaggtgg
     3001 ttcccgaaca gctcaccgac atccgttcgg agcgcccgca ccacgtggcg ggtagcgacc
     3061 ggaactcaga atgaggcaat gtagcgctgt tcatcacagt ttgcccgggc ttgagacgcc
     3121 aggccggtag aatcgccaac tcctcgtcac gctgggcccg cgagcggaac atgacggaaa
     3181 ttctttaacc gcaactgggc ggtccggtgg agggctgccg tgtgccactg cattggatat
     3241 aatgggtact gcgggatcgg gaggagcatc cgtggcgacc ttcatcatgc tgacgcgcct
     3301 cgcgtatggc gcgatccgct caccggaaca actggaaaag ctcgaaaaag aggtcatgga
     3361 acggatcgag gaggggcttg ccggcggcca agtgaaatgg ctcgccaact acgccgtgct
     3421 cggtccttac gattacctgg acatcttcga ggcgccggac atcgatgcgg ccatgaaggt
     3481 ctcggccatc gtgcgcacgc ttggtcatgc gcacaccgag gtttgggcgg cgactgaatg
     3541 gaaaagattt aaagagatcg tccgcgaatt gccgagcggc ggcgactgag ccgaagacgt
     3601 tcattcccgg gctccaggta ccgagcgacg tcgctgtcgc tcggtataaa agatcgctcc
     3661 atcctggctg gccgtgtttt agagccatat gaggccgtta atccggcatt attttattcc
     3721 ccgatgcagt ttgatattcc atttacgggt ctccacaacg atcaggcgtg ctcggaccag
     3781 cccgcgtatg gcctgtcaag cagatgagag aacgggaaat ttgtcagagt ttccgtgcag
     3841 caaagcacgt gacgccgacc atcccgccgc ccgagcccga cagcctgagt agctcactca
     3901 agcgcaacat caaggccctg gaagaccgcc gcaggcagca ggccgccgcg gccacgtgcg
     3961 aggagcggat cgcggaagca atcactcgct tcaccggcag catgcgcttc gtctatctgc
     4021 acgtggtcct tttcggcgcc tggtttctgg tcaacttggg ggtcgtgccg ggcgtgcccc
     4081 ggttcgatcc atcgttcgtg gtcctggcta tggtggcgtc ggtggaggcg atctttctat
     4141 ccaccttcgt gctgatcagt cagaaccgca tggcggacgc ggcggacaag cgggccgacc
     4201 tcgacctgca catcagccgc tcaccgagca cgaactgacg aagctgaccc ggcttgtcac
     4261 aatcgcaatc ccacttacag caggaaatta cgataaaccg tccctcaata tctgtactct
     4321 cgtcaggttt aataacaatc tccactatac cgctgctata gtcccgaaca attgtat
//
LOCUS       DATIFQ010000120        52149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1187911, whole genome shotgun sequence.
ACCESSION   DATIFQ010000120 DATIFQ010000000
VERSION     DATIFQ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 52149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52149
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187911"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1674
                     /locus_tag="VLE22_05160"
     CDS             295..1674
                     /locus_tag="VLE22_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSB13825.1"
                     /translation="MRANTPQLDQLQSMFRGQLIQPGDPGYDSARKVYNGMIDRHPKL
                     IAQCVDAADVIAAVNFGRENRMLISIRGGGHNAGGLGVCDDGLVIDLSRMRSVRVDPR
                     EHNVRVEGGCTWGDVDHAAHVFGLAVPSGTISTTGVAGLTLGGGIGHLSRKYGLTIDN
                     LLEADLVLADGSFVKASAKENEDLFWAIRGGGGNFGVVTSFLFRAWPVRTVHAGPTLW
                     PLEQATQAMQWYREFIVKAPEDLTGFFAFLVVPPIPLFPAELHGKKMCGVVWCYSGAP
                     EKADEVLKPVRTSVPPVFAHIGAMPFPILQSFFDALYPTGLQWYWKADFVRELSDEAI
                     DRHVEHAAKLPTGHSTVHMYPIDGAVHRVGKQDTAFYYRDVNWASVIVGVDPDPANKD
                     RIVSWAKDYWQALHPYSAGGAYVNFMMDEGQEGVQSTYQENYQRLAGIKGKYDRANVF
                     RVNQNIRPS"
     gene            1811..2320
                     /locus_tag="VLE22_05165"
     CDS             1811..2320
                     /locus_tag="VLE22_05165"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSB13826.1"
                     /translation="MDDLRAMQAPLKERYRSEPDAALITLKAEGRIGDRVTCSIQTGR
                     ALVEAGLHPATGGTGTFACSGDMLLEALAACAGVTMAAVATSIGVDLRSGKVRAEGDL
                     DFRGTLGVTKDVPVGFQRIRLEFELDTDASAEQLATLRRLTERYCVVYQTLRQAPPIS
                     VSLIGQMTK"
     gene            2493..2957
                     /locus_tag="VLE22_05170"
                     /pseudo
     CDS             2493..2957
                     /locus_tag="VLE22_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
     gene            3059..5134
                     /locus_tag="VLE22_05175"
     CDS             3059..5134
                     /locus_tag="VLE22_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSB13827.1"
                     /translation="MSPEQAQGKKVDARSDIFSFGAVLYEMVTGQRAFQGDSSMSTLA
                     AILSKEPKPVSEIAPATPHDLAKIISRCLRKDPEKRFQHMADLKVALAELKEESESGK
                     LAAQPVVAVTNHRRLLWISAAAVLVVAAVASTWFLRTKAPPPEVAMTSAPLTTYAGSE
                     SMPTFSPDGNQVAFVWNGNAQDNFDIYVKLIRTEPPLRLTTDPAGDASPAWSPDGRSI
                     AFLRATGDGAEVLLIPALGGPERKVAEVLRRPQRPSIPPLLAWLPHGDGLVFADASSA
                     DESTGLFLFSMKTGERRRLTSPPKASVGDSGVAISPDGEKLVFVRTFSFQNSHLYLLA
                     LSSDLRPVGEPERIATNLQACYNPAWTSDGKDIVFSAVEQGNERLWRIPASGSAVARP
                     LAAGEGGDDPAISRQGNRLAYRVGATDSNLWKVAISGQGTNHPVKVISSSRQDVNAQY
                     SPDGKRIVFASDRSGDMEIWASNADGSAPAQLTSLGAHSGTPRWAPDGRRIAFDSNKE
                     GRFQIYSIDAGGGVPQRLTRSPVDDAAPSWSRDGKQIYFSSKRTGRWEVWKMPAEGGD
                     PVQVTRQGGFSPFESPDRQLVYCQKTEGNSEVWKVPVDGGQESKVLDPVGGRQFAVAD
                     QGIYFIRYRAATPTLQVFRFATGETKEITRIGTPTMMGLSVSPDGRSLLYTQIDQVGS
                     DLMLVENFR"
     gene            5465..5950
                     /locus_tag="VLE22_05180"
     CDS             5465..5950
                     /locus_tag="VLE22_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB13828.1"
                     /translation="MSTRAALLAQRIGQGADTLAAFAEGLSDAQWHTVVPRDGRQVGV
                     IIHHVASIYPIEIDLAQQVASGNNIAGVTWGFVAELNAKHAHDHAAVGRQETIDLLRS
                     NGHSAAEAVRAFSDEELDRAAPVSLNADAPLTAQFLIEDHALRHSWHHLAKIKAVLGS
                     V"
     gene            complement(6428..7438)
                     /gene="galT"
                     /locus_tag="VLE22_05185"
     CDS             complement(6428..7438)
                     /gene="galT"
                     /locus_tag="VLE22_05185"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HSB13829.1"
                     /translation="MPELRQDPATKEWVIIATERMQRPHEFRRAAARPAQPEYDPNCP
                     FCPGLERQTPPELMARRNTGAANEPGWTVRVIPNKFAALVPQGNTERLLLDGFFLQME
                     GVGYHEVVIETPVHNRCIHRMSPAEVEEILHAYRDRYLGLRRDRRLKLILIFKNHGEN
                     AGTSLTHPHSQIVATPVVPAYVRRKCEEAIRYYDATGRCLYVDILKAEVRSGQRVILE
                     TDCFLVFHPFASQVPFGTWIVPKRHRPSFGQATIDELRDLATVLKESLSRLAKLLNDP
                     DYNYVIHSAPVDGEDDDYFLWHLEIVPRLTRIAGFEIGSGMRINTACPEETARAMREI
                     TA"
     gene            complement(7504..7785)
                     /locus_tag="VLE22_05190"
     CDS             complement(7504..7785)
                     /locus_tag="VLE22_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13830.1"
                     /translation="MKLSTKACALAFAALWGGCMLFVGLLNAVFSPYGVEFLRLMSSV
                     YPGYGAAPTVVSVLAGTIWGTLDGAAAGFLFGWLYNRFLMLGGPAGPAG"
     gene            complement(8256..8690)
                     /locus_tag="VLE22_05195"
     CDS             complement(8256..8690)
                     /locus_tag="VLE22_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13831.1"
                     /translation="MVSEKRPIMARPSLPDHDGVVALVITADESGRLFMTAALEASQR
                     RLRLAKDGIEARKVPATDSIPVVIMDGDAQAPSWSELLEQISVVEGALSPKFIVVSRL
                     RDDTMWAEVLNLGGYDLLPKPLEADQVSWVVRSALSGWNGAL"
     gene            complement(8710..9099)
                     /locus_tag="VLE22_05200"
     CDS             complement(8710..9099)
                     /locus_tag="VLE22_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSB13832.1"
                     /translation="MTRAQENRFKRAIEAKRDELIREICERRERLAIDPVSDPMDQVR
                     SIADRELLLRNVDRICAQLRSVEGALREIREGTFGICASCGGDIPTRRLAIIPWSPYC
                     VSCQERAEQSEGNEQLAEYQTPCALAG"
     gene            complement(9287..9637)
                     /locus_tag="VLE22_05205"
     CDS             complement(9287..9637)
                     /locus_tag="VLE22_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HSB13833.1"
                     /translation="MSTARRYGKNRWIVLAIALMLACLLVALPALAVQDQKETESVAL
                     ITGLDRGTYEPYNPGVIEQVQAVLKKNGLYQGDVNGGLDEPTMKAIGEFQKANNLTVS
                     GVPSPATRRLLLEE"
     gene            complement(9790..9966)
                     /locus_tag="VLE22_05210"
     CDS             complement(9790..9966)
                     /locus_tag="VLE22_05210"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSB13834.1"
                     /translation="MIFCGFTPAAENSNPDHEEIVRLAYSYWEARGGQEGSPEEAWYK
                     TEGALNGFGAAAGK"
     gene            complement(9963..10403)
                     /locus_tag="VLE22_05215"
     CDS             complement(9963..10403)
                     /locus_tag="VLE22_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13835.1"
                     /translation="MLPSLGSSVLETGIEVVLISPSETDSLPLRRLFGHSRSSVYWFS
                     TCQAALAFLREHPVGVVISNTELPDGCWTDLLGALTGFIPPPNLIVSSRLADDRLWAQ
                     VLNLGGYDVLLTPFDPDEALRVSTSAWLAWRQKLSEVPPPRKVA"
     gene            complement(10637..11107)
                     /locus_tag="VLE22_05220"
     CDS             complement(10637..11107)
                     /locus_tag="VLE22_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13836.1"
                     /translation="MSVALRLWAMLAVIALGLSAQKSNKPDFSGTWQLDLQRSRFGNV
                     PPPKSLTIQIEHNEPQIRIFTVTTNETGETNETLELTTDGKQHAYTLKGQTCAASARW
                     HWWRGQRLVVEVSCPENSRSRRFTLGAEGRMLTTVLTIRDRSVENKAYEFFFKR"
     gene            complement(11104..11652)
                     /locus_tag="VLE22_05225"
     CDS             complement(11104..11652)
                     /locus_tag="VLE22_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HSB13837.1"
                     /translation="METPDRAIPQELLDRAHCSVIVPGLKRGFLIFGARYGKGYVSCR
                     NKSRVGWTAPGTVRIEGVSFGPQVGGSETDVILLAMNERGMERLLSSQFMLGVGTSVA
                     AGVFAGVSLEGTTLRQDVSDNGELYGRTLEDREIIEKELQVPGLARRLISSLDKYSGR
                     QGSPPVIGQGGKRHESRTGGLQ"
     gene            complement(11755..12051)
                     /locus_tag="VLE22_05230"
     CDS             complement(11755..12051)
                     /locus_tag="VLE22_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13838.1"
                     /translation="MKTILSLFLGIVLIVAGTAVLTYQGITLTTAERQLLHVGPLQAA
                     TEQSNWTITLPPILGAVLLAGGTVIVFVIAMVARVPKGEKDSRPSPESRSMEQR"
     gene            complement(12336..12473)
                     /locus_tag="VLE22_05235"
     CDS             complement(12336..12473)
                     /locus_tag="VLE22_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13839.1"
                     /translation="MLIGDLHNSLGNASGGLGPVFRWVWASLIAVFVAGLVYMVSLAV
                     D"
     gene            complement(12558..15470)
                     /gene="treY"
                     /locus_tag="VLE22_05240"
     CDS             complement(12558..15470)
                     /gene="treY"
                     /locus_tag="VLE22_05240"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HSB13840.1"
                     /translation="MSQLRVPISTYRVQFTRDFRFVDCRELVAYLHGLGVGDLYSSPR
                     FRPQRGSSHGYDVTHPGRVNSELGTDEEFDDLCEKLKHYGMGLLLDIVPNHMAASHEN
                     SWWMDVLENGPGSPYAQFFDIDWRPATSKAAFLLEDKILLPVLGDLYGNVLENGKLAV
                     HLDESGFFLRYHERRLPLDPCSYAPILEQCVDQITGQIAAGHPSIDELRQLQSIVEGM
                     PRRTATEPVEAGRRRELSQSLKQRLFHLYRDHLEVRLAIDAALRDLSDTKDRPERADF
                     LDRILAGQGYRLAHWKIAYEEINYRRFFDINDLVRLRVEDEEVFRTRHEPILQLVKEG
                     KVTGLRVDHIDGLHDPQGYLERLQAELGGKGSPPSFYVVVEKVLGRDEPLPRQWRACG
                     TTGYDFLNVLNDVFVHPDGLRSLEETYANFTGDRMPFAEVCYARNKQVMWKLFAGEVH
                     ELGHHLGALAAQDRQARDVPLSELMNALVETTACLPVYRTYIRDFTVDERDRHYIERT
                     LDLARRRTSEIRVSTAAFDFLRRVLLLEPPAYAKDQKPQWLRFVMRWQQFTGPVMAKG
                     LEDTAFYAQHCLISRNEVGGDPLREGPPYSLTDFHSILKDQNEHWPYAMNTTSSHDTK
                     RSEDVRARINVLSELPDEWRNRLSRWNRWNGDKKTTVNGHEVPSPTEEVLIYQTLLGA
                     WPLEPDENRSFLERLKCFLVKAAREAKDHSGWLRPNQAHEEALVCFAESILDPTESNR
                     FLPDFLRFQERLALHGAFNALSQVLIKITAPGVPDFYQGTELWDFSLVDPDNRRPVDY
                     RTRIALLEQLRKRETENLRGLLREIVSGWKDGRIKLYLTDKALDFRRAHTDVFLAGDY
                     LPVEATGAKKDNLVAFCRRHETTWALTVAPRWTTQLTATYRVPLGERAWSDTALRLPE
                     GAPHSWRDVLSGEIVDVQLREGVRSVLRVRDLLRRFPVAVLTNTAESEHARKTPVR"
     gene            complement(15467..17917)
                     /locus_tag="VLE22_05245"
     CDS             complement(15467..17917)
                     /locus_tag="VLE22_05245"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HSB13841.1"
                     /translation="MERYVCIHGHFYQPPRENPWLEAIELQDSAYPYHDWNQRIAAEC
                     YAPNSASRILDADNRIRQIVNNYSRISFNFGPTLLAWMEQECPDVYRAILAADNESQR
                     RFSGHGSAMAQAYNHVILPLANNRDKRTQVLWGIRDFGHRFRRKPEGLWLPETAVDLE
                     TLDILAEMGIRFTVLSPYQANKVRPIGSRVWKDVNGGRIDPSIAYRIRLPSRRSLALF
                     FYDGPISKAVAFEDLLVRGENLAQRLNGAFADSRSWPQLVHIATDGETYGHHRRHGDM
                     ALAYALHFIEASSLARLTNYSEFLERYPPSHEVQVIESTSWSCAHGVERWKADCGCNS
                     GAHPGWHQNWRAPLRQALDWLRDQLAERFKEAGHEVFRNPWKARDAYIDVILDRSPDN
                     VSRFLETHANRSLQPADRVRALKLLELQRHAMLMYTSCGWFFDELSGIETVQVIQYAA
                     RALQLGQEMFGDGLEDGFLTLLERAKSNLPEHRDGRLIYQKFVKPAMVNLEKVGAHYA
                     ISSLFEEYPPKSGIYSYEVDREDYQRVHEGKAHLVLGRAKITSRITEESTTLSFGAIH
                     LGDQNVSGGVREYRGADAYGALLAEIQEIFHRGEIPELIRSVDQNFGSGTYSLRLLFR
                     DEQRKIVGLIMEKALAEAAFLYRGFYSNYATLARFVTELGIPLPARFQMAVDFTLHED
                     LSAALSTDEPETNQVQALLDQVNRTGILLDMVTLEFAFRKTVEHAAEAFHRNPDERQL
                     LERFHKVVSLCPLLPFQVNLWAAQNVYHAVREARFEELQAKANGCDSAAQEWVRTMGA
                     LGAKLGFYFEPRTAEAAK"
     gene            complement(17918..19774)
                     /gene="treZ"
                     /locus_tag="VLE22_05250"
     CDS             complement(17918..19774)
                     /gene="treZ"
                     /locus_tag="VLE22_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HSB13842.1"
                     /translation="MEPMDFGFRHGTLHLGEGVTRFRVWAPQASDVELRITYPQRFAV
                     AMHQLGAGYWEVVPGNAPPGTRYLYRINGRLNRPDPASRFQPDGVHGPSEVVNAEFHW
                     QDRAWWGLPIEQCVLYELHVGAFTPEGTFDAIIPCLEYLRDLGVTCVELMPVAQFPGE
                     RNWGYDGAYPFAVQNSYGGPQGLKRLVNECHEHGLGVVLDVVYNHLGPEGNYLSDFGP
                     YFTENYKTPWGPAINFDGPGSDEVRRFFIENALYWVREFHIDALRLDAVHGIFDRSAY
                     PFLEELGDAIREEATRLNRRVHVIPESDLNDPRLIRSKDLGGFGLDAQWADGFHHALR
                     VLLTDDRAGYYEDFGELRHIAKAFEEGYVYSGEYSRFRRRRHGASALDIPAYKFVVFS
                     QNHDQVGNRMLGERLGNLVSFEDLKLAAAAVILSPHVPLLFMGEEYAETAPFLYFVSH
                     SDANLVEAVRRGRKEEFAAFAWQGDPPDPQAEGTFLRSKLNLELRQKGEHGTLLGFYR
                     ALLHLRREVPALAFLSREQMSVVTDEEAKVIFVRRWRDNDEVILVLCFAETGVRVKLP
                     VATGRWSKLLDSSDATWFGPGSDLPEELDSPNDMELALKRRSAVLYRRCVEA"
     gene            19979..20149
                     /locus_tag="VLE22_05255"
     CDS             19979..20149
                     /locus_tag="VLE22_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13843.1"
                     /translation="MQVWVAWTTVVGPHRSRYVALETGEEIAKKKTRKGGGGFRRHIV
                     EIEFLWRTRVLR"
     gene            20454..21875
                     /locus_tag="VLE22_05260"
     CDS             20454..21875
                     /locus_tag="VLE22_05260"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HSB13844.1"
                     /translation="MEPQSPAFRTRIEHLLAIDRESKPKVYLQVFDAAEVFSLNYALE
                     LLLSAGIATLGLVLDSPAVVIGAMLISPMMGPILAGGLALAAADIYLGIKSLLSVVLS
                     SLCAILFAAALVWLLPFHSPTQEILARTHPNLLDLVVALFSGLAGSVVVCRGGGGGGV
                     TALPGVAIAVALMPPLCTVGFGVGSGFSWPIITGAGLLFLTNLAAIMASAFVTFYAVR
                     MDAPELRMKIDYSILEHAASDRLYRLLMKTRLARTFGDIGKLRWRVLMLVATLVALFI
                     PLRQSLFQLREETIARTAANEAVRLMASPGMIFTQQLDVTPERIILRMIVTETVPEDR
                     IREAEKLLLRRTGKEVTVAVRKVASEEELAMLRERFRTPPAPPQPRDLNSMRTELIGR
                     VEQPLKEVWPTELAPLVSYEVGFTPEDILVRVRYKSETPLDATAEAMLTKVLQTRLNE
                     PKLRLILEHEATPKSRTRRSVRK"
     gene            21971..22105
                     /locus_tag="VLE22_05265"
     CDS             21971..22105
                     /locus_tag="VLE22_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13845.1"
                     /translation="MLDTNLTLQIIESTVFLFPKLLAEVVILVTGFWLVQYMSGSMVV
                     "
     gene            complement(22475..22612)
                     /locus_tag="VLE22_05270"
     CDS             complement(22475..22612)
                     /locus_tag="VLE22_05270"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HSB13846.1"
                     /translation="MAKIDVLEGGGMKLDPKFFLQFGSMRAHQVRLEGGDASSDSYCY
                     S"
     gene            22855..24198
                     /locus_tag="VLE22_05275"
     CDS             22855..24198
                     /locus_tag="VLE22_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSB13847.1"
                     /translation="MRMRSWPFLLMGFGALLVLIGLSSAALHHRMEQVRAEVQALQQA
                     NEETSRVLNDLRSEIYLFAVLLRDYLLERSPEAAAQQRQALGELRNSTQDHLRALQGK
                     AVSRDRPEVQRLRNVIEEYWRSAEPVFGWTPAQKASEGSVFLRRRVVPQRQAAFQLAS
                     EIENVSLSQSRLRQQEVLRTQEGLKGYLRRVAAAALILGAVVAMVSVARTRSLEASAT
                     RHLRLIERGAEDLRRLSLRLSKAQEDERKSISRELHDQVGQMLTALRIELVNIEEFRQ
                     DAGGGFAQHMADARRLAEETLRTVRDISMGLRPSVLDELGLGPALKWQGREFTRRSGV
                     AVEIQIDGSLDKLPDTHRTCVYRVVQEALTNCARHARASLIRITVHGGATAIALTVED
                     NGVGYNVPADSSRGLGLLGAEERVRELGGRMEIFSRPSKGTLLRCEIPTSPEVKV"
     gene            24195..24848
                     /locus_tag="VLE22_05280"
     CDS             24195..24848
                     /locus_tag="VLE22_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB13848.1"
                     /translation="MTTKTKILVADDHGIVRHGLRSLLERQQDMEVVGEARDGREAVR
                     MAKELDPHIIVMDIAMPQLNGIDAAEQVLRTSPPTKIILLSMYADEEFLTRALTVGVK
                     GYLLKDSAELDLVRAVRAVAAGKSFFSPAIAQALAEDYVRQLQSKGLQDSFELLTSRE
                     KEIIQLLAEGKSNKEVATVLNVSPYTVETHRMHLMQKLNLHNTAEIVLYAVRKKIIT"
     gene            25121..26449
                     /locus_tag="VLE22_05285"
     CDS             25121..26449
                     /locus_tag="VLE22_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSB13849.1"
                     /translation="MSNQPEQGRPGSTRRQFVGAGAAAVAVLGAARGSAETLAIDGGA
                     KTVTVPDEHQAAISKWPRYGPEEKQALVELLDNNRSYDEIPLFENELKEYLKAPYVKA
                     HCNGTSALTSMFFALDLPRGSEIMAPSYTAWATTAPMQFFGYVPVFIDINPRTMTFDL
                     EYAKKRLNPRVRAVLPMHSFGNPCDMDDICEFGRKHGLVIVEDAAQAQGASLKGRQMG
                     TWGSIGAFSFQNSKILPAIEGGCGVYQSREHYERATTFGNYELPRSFPEDSPYRKYGG
                     TGMGLKFRIHPLAAAIARRQLRGMDERNARLDAQVRKLTDRLIELPGLSRPLTRTDAK
                     RVYWAGLMLFLDDAKARCSRGTLMKALQAEGVRIAPGAYDEQHKYVLYSEAKWWDHPP
                     VIPSDLHGTTEVNRLAVRLPLFYEEAPELIDQYVKAFEKVWAKRSQLARA"
     gene            complement(26568..26699)
                     /locus_tag="VLE22_05290"
     CDS             complement(26568..26699)
                     /locus_tag="VLE22_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13850.1"
                     /translation="MLLLILFFHNSEDENRYVARDLFLFEDRFLVAPLLTKGSVRDV"
     gene            complement(28350..30086)
                     /locus_tag="VLE22_05295"
     CDS             complement(28350..30086)
                     /locus_tag="VLE22_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB13851.1"
                     /translation="MNPTGNTASLDALPEWARRLSEKYYSRTIAMFVLHGNVHDLVAW
                     KRGGHTEYAPLAKFLDEGLFGRRDLVLSYDRGGGLGFANSEVQQDFQRALAGYDAYHG
                     TKYAQGLPRNPDGVLALIESYLRLRVLDRKKIALSIGYADTIAPAGDASSMGSEDRNC
                     LVILKRWAQNQSFLRADVTLCLVTESLAGLNAGLVQNPGVAAIEIPLPNEQERTDFVQ
                     AQMEATPLPPGSDVTAELLAKLGAGLKLVQLQNLIANACENRQPLTIPFLTKLKKELI
                     EAESGGLLEFVQSKYDLSVVAGHEAVKKKLRDAAAALRAGRFDVLPMGYVICGPVGTG
                     KTFLTTCFAGEVGIPAVMLKNFRSMWQGVTESNLERVLTLLKAMSPIAVVVDEADAQL
                     GTRSNFGDSGVSSRVFAQIAQFMGNTEYRGKVIWFLLTCRPDLLPVDLKRQGRAEEHL
                     ALFYPHTDEERLAMLRAMQKKTGTRVASEEAEKFFLAQAGTLSGADTEALLVRARMKS
                     ALEGDAAVDVEDLKAAIADFVAPSYPTEIELQNMVAVLECTSKSLLPDAYRDMDRGEI
                     LRRVGELGVLSR"
     gene            complement(30089..30994)
                     /locus_tag="VLE22_05300"
     CDS             complement(30089..30994)
                     /locus_tag="VLE22_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13852.1"
                     /translation="MLDDKPSFGAEVKGVVNRKLDEAGLSQHDYVKAALKWQYNWIGL
                     AGAALFALISGTGLPLVLAAGLEMMYVALVPQSNPFRRLVRSWKYADQKREHQKRLNE
                     MYRNIPAEMRSRYTNVLQVAEAIRANYSQLSYASQIFATQMEAKLAGLLEGYVRLLYA
                     AHTHREYLKSLNPDQVRNEAATLEQSLAKAPAKVQEINQRRIEILRKRVAKFEKIREN
                     SQVIEAQCSAIEDVLQLIRDQSVTMRDPQEVSAQLDHLVQDVEQTEQSVKEMEEIFAL
                     AAPDADEPHLPMAEQSPPPRDRVRN"
     gene            complement(31057..31881)
                     /locus_tag="VLE22_05305"
     CDS             complement(31057..31881)
                     /locus_tag="VLE22_05305"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB13853.1"
                     /translation="MEPKNRITLRNVSRVFTGASGKKVEALRDVNLEIADEFAADGRD
                     IGEFRVLLGPSGCGKSTILRMIAGLDFPTAGEVMVNGRRVVGPGPDRGMVFQKYTSFA
                     WLSVARNVEYGLRIKGTQETDRRQIVEHLIEAVGLSGFENAYPHTLSGGMQQRLAIAR
                     SLAVRPQVLLMDEPFGALDAQTRNDMQTLLLRIWDETAATVLFVTHDVAEAVFLADRL
                     YIVSARPGTIVEEIPVPFVRPREQGLKEQREFQELESHALARLRRTAGSGQVRVSL"
     gene            complement(31865..32698)
                     /locus_tag="VLE22_05310"
     CDS             complement(31865..32698)
                     /locus_tag="VLE22_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSB13854.1"
                     /translation="MVQPTSIRKPIARPLTLVLGFAAWTVLVAVWFLVTRSNLLPPLA
                     LPEPVGVVKAIARLWVEYDLLGNVFQSWWRIAQAFCWSAVVAIPLGLLMGSFPWVFHL
                     VNPIAAPMRSMPITAFLPAFIALFGMDEGMKVGFLFFGMFFYLLALVVEQVSRVDHAL
                     LETAYTLGAKVHHVLWLLFRASFPAIFGSFRILYDIGWTYVILAEMVNARKGVGYMVE
                     AARKVLDFERVYAGIIAIGVAAFLFRWLLTVLEDRLFPWQRPAREQSEPAISSAHGTK
                     K"
     gene            complement(32698..34254)
                     /locus_tag="VLE22_05315"
     CDS             complement(32698..34254)
                     /locus_tag="VLE22_05315"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HSB13855.1"
                     /translation="MAVRIEKGGWIFIFLIGLGLVAYGLDRYGVVNLSGLFGKKAATT
                     TVDTSKPLATTGDRETNQVRVRVNIWVGCVGGLVANGGLDTAAGSIYDKKGLKVSFKI
                     IDDWTEGTAALASNNVDVMLTTADVWAKDYGQLLDKGFRARAVYMVDWSRGADGVIGK
                     QGINSIEDLAGKSVAFAPYTPSHFLLWNGLKSSGLNTEQRNEIFTQAVHTKDGIEPAT
                     LFAQQKVDAAVAWDPDMTDAVGKRPGAKKIYDTRVANRLIADILVVSDRFSANSPQTL
                     RNFLEGWLEGVEFIRQQPTRAHTLIGTIKDFNIPEDLAKTMLGGVRLSDYADNKAFFG
                     SGAGSDYDNIMGMAQDMYRELRLIKGAPDIEGSVDRRYIAAMEGKFSSQSTEAPIEYK
                     APPKGATPIATQRRSIYFETNSAALSPDSRAVVEEIGGFMRAYENTVVDIDGNTDSTG
                     SRELNLTLSRQRADTVKNYLMTKYGYPASRMRTAGNGPDKPIETNETPEGREKNRRTD
                     IKVYPNPANQ"
     gene            complement(34270..35001)
                     /locus_tag="VLE22_05320"
     CDS             complement(34270..35001)
                     /locus_tag="VLE22_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HSB13856.1"
                     /translation="MFKRLSRILRSFFGFFISVAEDPELILEQNIRDMNDQVPRMNES
                     IAMVKANATLLEKEENKYKADVNDLATKVKAAIQAGRDDLAGHFAVQLEQVRSALART
                     QGQLATARAGFEKAQNVKAAFLREKERKTREAMNAIRDYRRSQWQKKVADAMEQFEVA
                     GVSQTHDEMVRKIEETTAVNEARMEMALGNVDQQKFQIEEEAEKLRASELVKQFKAEM
                     GLISPEASTPAPEKTIGGRETEKTA"
     gene            complement(35023..35712)
                     /locus_tag="VLE22_05325"
     CDS             complement(35023..35712)
                     /locus_tag="VLE22_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13857.1"
                     /translation="MSKKWYNYFVSVDPVNPGAGAGGEPAPDTPPGSARTAAQTIADI
                     AAAAKATPTFTQPVADPTAFEQIYQAAEVRTPAHGFTIFKIADMLKSEHIRSLPVEIK
                     RSSVLLALDAAGVKLQEIIEDAVRRDRALDAFELVQQRALDQLEAKKSDENKKLQQEA
                     DRVLTELRASIQANNDAVARERERMQTWRLQKQQEERRIADAVAPFATENPITTGPAA
                     APAKPEETPGS"
     gene            36176..37561
                     /locus_tag="VLE22_05330"
     CDS             36176..37561
                     /locus_tag="VLE22_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HSB13858.1"
                     /translation="MTKSEQFSWRRRDFLGATLLPVVCHAVAAPDDMVARSVKNLKLS
                     VSRNGRFFVDQNGKPFFYLGDTCWLLFQRLNHAELDEYLKDRAGKGFTVIQAYVIRGL
                     DKKHPDGNSSLLGESPFIDRDPTKLNPAFFANVDYVINRANELGLVMGLVTSKSWHVN
                     EHPERVFDATNAFTFGKLLGQRYRNNAVLWYVGGDSPPGKDEATWVAMARGLKEGNGG
                     SQLVCYHGQGSTSSSMWFHNADWLDFNSIQSGHHFGSDSYAFVSKDYALTPAKPTVDM
                     EPAYENHPTGANKPRVDAHKVRSQAYSAMLAGAAGHGYGTLDLFYLYKDADGPFPKNG
                     FQHWRTAMAYEGSRQVGLMRRLFEQRPWHKMVPDQSALASAPGDGPYRLVAARAEDGS
                     FVIAYTPRGGSVSIHMNKFNGSPVKAQWYDPRNGTWKAIGQYASQGIQEFVAPSHGEQ
                     DDWVLVLDVVP"
     gene            37997..39265
                     /locus_tag="VLE22_05335"
     CDS             37997..39265
                     /locus_tag="VLE22_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13859.1"
                     /translation="MRVLLEDAIAQWRACDASFPQATPRFSEHEQAPREEQLARFLGS
                     LEAEVARLPRTRSDRDGVRERLTGAFVRLAKSGLGLEDRHLDLLLGGGFSGVGTALAR
                     RARRFDPAVSTADILQACRNAWTACGLQVLLGRPMEVTPSIFAYSMLYPYSDNYLDDP
                     KVTREEKVSFSSRFGRRLEGDSVSPANAHEENIWRLVGLIEGQYLRSLYPQVYSSLLA
                     IHLAQFDSLRLSRSGAAAGEVDVLRLGFAKGGTSVLADGYLSAGSLTAEEARFIFLWG
                     VVLQLGDDLQDVRDDQAGGILTLFSQAAGKKPLDAVTSRTFHFGERVMSLLEGVGVAE
                     SGTLKELMRRSSRSFLLRCAGEAGEFYTPEYLARLEACSPYRFSFLNRRREQFTRRQA
                     AFTRLFEAFLAAGDDEPAFPLLPSGLMPRL"
     gene            39582..41519
                     /locus_tag="VLE22_05340"
     CDS             39582..41519
                     /locus_tag="VLE22_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HSB13860.1"
                     /translation="MMLGPGRMACSHWLGGWLPVWTNRSRAVLFALVCSPLMSAATCY
                     AQEWRFWGGDAGGTKYSPLEQINKKNVSVLKVAWTYDTGDWSDGSEYPTRSAFEVTPL
                     VVDGVMYVSTPFCRLIALDPETGEKRWEFDPKIDRAMRTNLFVSRGVTFWQDGNKKRI
                     FLGDLHGCLFSIDAMTGKPDPKFGKNGMLDLRAGLADQFPDKQYKLTSPAAVCGKVVI
                     TGSLVSDGTPKGPSGDIRGFDARTGELLWRFHTVPRPGEFGNDTWQGDAWKDRGGTNA
                     WSVMSVDEKNGLIFVPLTSPSYDMYGGDRKGANLFGNSVVALDCRTGKRRWHFQTVHH
                     DVWDYDLPAQPVLVTVQRQGKDVPAVAQVTKMGFTFVLHRLTGAPLFPVEERPMPKST
                     IPGEETWPTQPVPVKPPPFARQSVTRDDITDVTPESRKECLEILGDADVDVKIYDPFG
                     EQDAIIMPGLNGGTNWGGASFDPTTRLLYVNSMDVGGLFRLVKRPEGSEIPYALRARK
                     YEFLWDSKGYPCQKPPWGRLTAIDLNTGDFRWQVPLGEFDELKARGVPKTGTPNIGGS
                     VVTAGGLVFIGATNDGKFRAFDKDTGAELWITSLPASGMATPTTFLGKKTGKQFVVIA
                     AGGGNKYDKAFTGKLVAFALP"
     gene            41530..42054
                     /locus_tag="VLE22_05345"
     CDS             41530..42054
                     /locus_tag="VLE22_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSB13861.1"
                     /translation="MQSVAFAILAALLLVSLLAQRAPEYKGKDEKLPQAVAPQPVAYS
                     HKKHVALGMRCLDCHKGAAQEDQAGLPGTALCMDCHATIKAESPEISKLAEIHKRGEK
                     LGWVRVYRVPGFVFFSHASHVKAGEQCITCHGQVQARDVLAKEVSTGMIACMNCHAAR
                     KVSNDCVLCHQLSF"
     gene            complement(42363..44108)
                     /locus_tag="VLE22_05350"
     CDS             complement(42363..44108)
                     /locus_tag="VLE22_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13862.1"
                     /translation="MHSLCRVLSVGLAAVSLQAVSLPGGYYRILEAGADKVESRLNAQ
                     PDADLKTLESQSGSRHFPYAILAPTVLYAKQHRDNPRYHDPKMLALALRIGDLLASED
                     EKGTFEPRGDSDWDTYMWLEAYRLLEKELGEERRARWKRAIERNLALLVDDAKARLDF
                     PWYNSPYIGTSPNHYAQWASNLLLAGQVFRNKEWEELGARILQRFATIEQTPDGYWGE
                     HSRRGPTTGYNHLTLTSVALYWELSKDPAALKALRRVTDFHKYFTNPDGSPVEVIDDR
                     NRNRTVSAWGHFAFSNFADGRGYAEFLANFFKPETLTIDQLGRLAQDALYYHDGPKSP
                     APQEQSRYFHQMEIPAGIRKTGPWVVVLSGIVDTQAINSRFYLDRQGNLGVFHEKLGM
                     IINGGNSKRQPELATFSEKLMGQIVHMPLSSRLQMSDAQDRLSLAYNTFWVDLLMGPP
                     SDKEMSFQVVVNGRGRPSDETLLNLQLVLKSGAVLETGAGRKITLGAERFELSAAELG
                     GLIRHGGWTMSVDPAARLSWPVFPHNPYADAPETSLDQAVGRLTIPLRTKFVPGGYVK
                     PAEQQVRFKITAGNP"
     gene            44238..45110
                     /locus_tag="VLE22_05355"
     CDS             44238..45110
                     /locus_tag="VLE22_05355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB13863.1"
                     /translation="MRLIDRDRRSFLKSCVAAPLAAVEVPQAKPGYRTWDLHVHMFGF
                     AGATPEELIESLVRFADRMEVERWVLSNPLHADPTPEQFRDANDAVLRAIKRAPDRTL
                     GLVYLNPNHLQASLDELDRCVRDGPMIGVKLWIARHCNAPELDPIVERAAQYQSIIFQ
                     HTYLKNQGNLPGESTPSELAELARRHTRAKIILGHTGADWERGVRSVRDLKHVTVDLA
                     GSDPTAGFTEMAVRELGAERVLYGSDVGGRSFASQLAKVLGANIPEQAKIDILGGNLR
                     LLLAPIMRAKGMRV"
     gene            45107..45877
                     /locus_tag="VLE22_05360"
     CDS             45107..45877
                     /locus_tag="VLE22_05360"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB13864.1"
                     /translation="MIIDTNVHLSRWPFRRLADDEPGALVTRLRRHGVIEAWTGSFDG
                     IFHKDIGGVNARLEEDCARYGRGLLIPFGTVNPTLPDWREDLRRCREVHHMPGIRVYP
                     NYHGYTLDDPKFTELLRAATDSGVIVQLVVQMEDDRMQHPLLKIPAVDLAPLPTLTQT
                     HPKLRLMLLNGFRSLNGGAVKTLVNAPGVRFEFAMLDGIGGVGDAVEAVSANRLLFGS
                     HAPFYYFESALLKLKEAGLPKEVVDRICQANARELLPR"
     gene            45904..47079
                     /locus_tag="VLE22_05365"
     CDS             45904..47079
                     /locus_tag="VLE22_05365"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HSB13865.1"
                     /translation="MDDQNAAGARVAATADHPAEPVVAPVKERLQSLDVFRGFTLLGM
                     VLVNSHPGKIFPGLGHASWNGWGFADLIFPFFVFIVGVAMPYSFASRLARGDSHSKLF
                     WQALRRSVLLFVVGVFLNGYPQFDLATLRVMNVLQRIAICYFLASILYVHLRLTARSM
                     VWLCGTILVLYFVLMKFVPVPGFGAGVLEPVGNWGNYIDQLVMPGRLGHKTWESKSLL
                     GCVPALVTMLMGLLTGIYLRTRRPAYEKLANLYFYGSLCMAAGVIWTPWFPINQHLWT
                     SSLVLFMGGVALVGLATCYYIVDVRKVTWWTLPFLIFGVNSIAVWVFSQLGMKTLMAI
                     QTVAPDGTATNLWKAGGDILSRHLGEMGGPFVFAVLFDLFWLGVMGILYWRRIFIKL"
     gene            47268..48482
                     /locus_tag="VLE22_05370"
     CDS             47268..48482
                     /locus_tag="VLE22_05370"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB13866.1"
                     /translation="MFIPRRVLGAPGRPGANDRIRVGIIGVGNRGSLLIDQLPEGAEM
                     VAAADCYLKKAYDGASKRQAKWHIYQDYRKLLEQKDIDGVIVATGERWHVLCSIHACQ
                     AGKDVYVEKPMSLYVEEGRVLVRAARKLKRIVQVGSQQRSMAMNQLACDFVRSGGIGR
                     VFFVEGKNYGSPQPPQSVESSAMSEPVPEGFDYNMWVGQAPMRPYSSKGGGGWGWDFG
                     GGDMTNWGPHGLDQVQSALGMDGSGPVEFWLLAEGPGPEGSMAFRYANGVTVRLEIPT
                     GDVQGGAIFVGEKGRIDVIRNMFRTDPPQMVRQLPPPEEVQKWRDDVALWQAKYHMQN
                     WLDCMRTRKLPLADVEIGHRSVTVCHLATITRRLGRMLRWDPQAERFIGDEDANSLLS
                     RPRRKGYELPTV"
     gene            48539..49279
                     /locus_tag="VLE22_05375"
     CDS             48539..49279
                     /locus_tag="VLE22_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13867.1"
                     /translation="MKRIGFCLTVFVAGVLFGLGGLRLWDHSTAVAQGLGTAYPPHPA
                     ASRPGITIDPTAFVHPSVILEGTINIGPYAYLDAGSILTGNVTVGHHSLIRCNVTIRG
                     KVRIGNYTHIYDNVNIEGGRPAKPLGGSLAEVADEAIIGDHCWINHGAIMHGTRIADG
                     GAVGLGAVCDYNTRIGKDAVLADGSATRVDMVICDGCFFEGVPAVVKRKGLTEKDRAE
                     YFGVSPLAWTTFEGDRQEANAKKRMGQK"
     gene            49416..50534
                     /locus_tag="VLE22_05380"
     CDS             49416..50534
                     /locus_tag="VLE22_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HSB13868.1"
                     /translation="MHLYTYAVLTGTFVCGLLLEAQEPPRPLISPEVQQDRRVTFRLR
                     APNAEQVFLALDGSKRAAMQKGEDGVWAITAGPLEPDIYSYSFVADGVTLLDPSNSSI
                     TLNLLNPASSVHIPGEGLAWDVTNVPRGVVHHHFYRSNIVGDDRDFYVYTPPGYDPRA
                     KKPYPVLYLLHGFSDDAGTWTSVGRAHVILDNLIAQGKARPMLIVMTLGYGAPEILSR
                     TRPAFRDPGLRQRSFEKFRDSLFEEVMPRVEKEYRTSNDRKMRAIAGLSMGGAESLYI
                     GLNALDRFAFIGAFSSGGLGDDLAAQFPKLDEEANSLLRVFWISCGTEDRLLDFSRKF
                     VEFLKTKKVEVTLREVPGSHNWRVWRRDLANFASMLFQ"
     gene            50710..>52149
                     /locus_tag="VLE22_05385"
     CDS             50710..>52149
                     /locus_tag="VLE22_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13869.1"
                     /translation="MKRLVLSLLLAVLVLPAADLYHGKVYGIFDPLKVSETPVSITVS
                     GGTFRYIIDRATGQITSVKALEDEFVAAGGAFPNPYVGLMPEDDPGARREGGKERPRY
                     GYEKSVEMRPLLWSGELTDAHRFDAAKGKGIRTELVRSAADSVEVRSSGRYGDTPLSW
                     NINYLVDVDGFTKVTVTLSTDKAVQLRWHCFNHAVLAKNVIQYLTRVSDPERPPIEVR
                     PLPTVSIGDVQPDSPILESHWNAFFHLANRVTGIEFSKQDFEDRYSGYRDSAVILESG
                     TSVDTGAVGTKDGRTLRGWDSRGKTNIFTQIYQREQGLEVEEFDIRNATFPLNPGDVR
                     TRVFWVQPTPAKRPRNDLNSLRVVWPGPHQIVMTRWRGRQQPWSPPDDEQVKLWAQMG
                     VNFIVGGADYWSGDYARPLAPEKIRHFIETAHRYGIKVIPYVTFTDFNFTAPGYQEHA
                     AEWMASKSIEYANETTLMCYNAEGWRNHLE"
BASE COUNT        10283 a        15696 c        15775 g        10395 t
ORIGIN      
        1 ctctgcttcg aagaactgga agtgtcgttc ggtgtagggc caccacccga ccgagtagcg
       61 cgccgggatc gctatcttgg aatgggaatg aggctacctt tcagcgcaga agatcttctt
      121 gatgtctttc gaggctacaa ctaggcggtc tgtttctctg ggagggacac cgcgggtcgc
      181 tttcggtcat tgcgatcccg gatcagattg tcgcctccgg ctgccgagca tgaggtgctt
      241 cacctctgga ttccagagag gaatgtctca ttcaaaagtc ttaacctgat gcatatgaga
      301 gccaacacac cacaacttga tcaattacag tcgatgtttc gtggccagtt gatccagcca
      361 ggtgacccgg gctatgacag cgcccgcaag gtctacaatg gcatgatcga ccggcatccc
      421 aagctaattg cgcaatgtgt tgacgccgcc gacgtgatcg cagcggtgaa ttttggacgc
      481 gaaaaccgga tgttgatatc catccgcggc ggtgggcaca acgccggagg cctcggggtc
      541 tgcgacgatg ggctcgtgat cgatctctct cggatgcgga gcgtccgtgt ggacccgagg
      601 gagcataacg ttcgcgtcga gggaggatgc acctggggcg acgttgacca cgctgcgcac
      661 gtattcggac tcgctgtccc gagcgggacc atctccacta ccggcgtcgc cggtctgacg
      721 ctcggcggcg gaattggcca cctctcccgc aagtacggtc tcaccatcga caatctgctc
      781 gaagccgatc tcgtgttggc cgatgggagc ttcgtgaagg ccagcgcaaa ggaaaacgaa
      841 gatttgttct gggctatccg gggcggcggc ggaaatttcg gcgtcgtgac atcgttcctg
      901 ttccgcgcct ggccagtaag gaccgttcac gcgggcccga ccttgtggcc gctcgaacag
      961 gcgacgcaag cgatgcagtg gtaccgcgag ttcatcgtca aggcaccgga agatctgacc
     1021 ggcttcttcg ccttccttgt ggtgccgccg ataccgctct ttccggccga actccacggc
     1081 aaaaagatgt gtggagtcgt ctggtgttat tccggtgcgc ccgagaaggc tgatgaagtt
     1141 ctcaaaccgg tccgcacctc ggtgccgcca gtttttgcgc acatcggtgc gatgccgttc
     1201 ccaattctac aaagcttctt tgatgcgctc tatccaacgg ggcttcagtg gtactggaaa
     1261 gctgatttcg tcagggaact tagtgacgaa gccatcgacc gccatgtcga gcacgctgcg
     1321 aagttgccga cagggcactc cactgtgcac atgtacccga tcgacggagc ggtgcaccgc
     1381 gtcggaaaac aggatacggc gttttactac cgcgacgtca actgggcgag tgtcatcgtc
     1441 ggtgtagacc ccgatccggc aaacaaggac cggatcgtga gttgggccaa ggactactgg
     1501 caggccctgc atccgtactc cgccggtggt gcctatgtca acttcatgat ggacgagggc
     1561 caggagggcg tccaatcaac ctaccaggag aattaccagc gcctggcggg aatcaaagga
     1621 aaatacgacc gggccaacgt ctttcgcgtc aatcagaaca ttcgcccaag ctgaaacccc
     1681 ggtccgggcc tcaacgcgtc acagcgacca tcgaataacg atcaccagtg gacatccggg
     1741 cctccaggtg aagctcgttt ctccaggttg cggcttgaga gagcgatcta cataatggaa
     1801 tcccatgaac atggacgacc ttcgcgccat gcaggcgcct cttaaggaac gctatcggtc
     1861 agagccggat gcagccctca tcacgctcaa ggcggaggga cggattggcg atcgagtcac
     1921 atgcagcatc cagaccggca gggctcttgt cgaggcgggc ttgcatccgg ccaccggagg
     1981 caccggtacg ttcgcctgct cgggcgacat gctgctggaa gccttggcgg catgcgctgg
     2041 cgtcacgatg gcggccgtgg cgacctccat cggcgtggat ctgcggagcg gtaaggtgcg
     2101 cgccgagggc gacctggatt ttcgcggaac gcttggcgtg accaaagatg tgccagtggg
     2161 tttccaacgc atccggctcg agttcgaact cgatacggac gcctctgcag aacagctcgc
     2221 aacactgcgc cgcctcaccg agcgctattg cgttgtctat cagacgcttc gccaggctcc
     2281 ccctatttca gtatccctga ttggccagat gactaagtga cccaaattcg cccgagaatg
     2341 tttgatcgct gaagcgcagc cgaccccccg tgacgtatcc tggccggatc gtggacaatc
     2401 ctctgaccag ttagcagttg tttgattctc aaagtcccgt cttttagcag ttgcggtaca
     2461 atcagcagaa gaatccttcc gggacctggg aaatggttgg cctaacaatc gggcactacg
     2521 agatcatcga caagctcggt gaaggcggta tgggcgtcgt ttacaaggcc cgcgatacgc
     2581 acttggatcg cctcgtcgcc atcaaactgc tgccggcgga caagatcgcc aatcccgaac
     2641 gaaagaggcg ctttgttcag gaggccaaag cggcttcctc gttgaaccat ccgaatatca
     2701 ttactatcca cgacatcgcg aacgacaacg gtcatgactt tatcgtcatg gagtacgttc
     2761 cgggcaaaac gctggatcaa cagatcccac gccacggcct gagagtgaac gaagtgctga
     2821 aatgcgccgt gcaggtcgcc gacgcggtgg cccgcgcgca cggagccggg atcatccatc
     2881 gcgacctcaa gcccggcaac atcatggtgg acgagcacgg gttagtgaag gtgctcgact
     2941 tcggtctggc caagctgacg gagacggccg aaggcggcga agaccccgaa acacgtacga
     3001 tccgtgacga ggaagcgccg cgtaccgggg aaggcaggat catgggcacg gccgctacat
     3061 gtcgccggag caggcgcaag gcaagaaagt cgacgccagg tcggacattt tcagtttcgg
     3121 cgcagtgttg tacgagatgg tcacagggca acgggcgttc cagggcgact cgagtatgtc
     3181 gacgctcgct gcaatcctga gcaaagaacc caagcctgtc agcgagattg cgccggcgac
     3241 tccacacgac ctcgcgaaga tcatcagccg ctgtctgcgc aaggatcctg aaaagcgctt
     3301 tcagcacatg gccgatctca aggtcgcgct tgccgaactg aaggaggaat cggaatcggg
     3361 gaagttggcg gctcaacccg tcgtcgcagt aacgaatcac aggcgcctcc tctggatctc
     3421 ggcagccgcg gtgctggtgg tcgcagctgt ggcgtctacc tggtttcttc gtacgaaggc
     3481 cccgcctccg gaggtcgcca tgaccagcgc ccccttgacc acgtacgcgg gctcggagtc
     3541 catgcctact ttttcgcctg atggcaacca ggtggccttc gtatggaacg gaaacgccca
     3601 ggataacttt gacatttacg tgaaactgat ccgaacggag ccgccactcc gtctgacaac
     3661 cgatcctgcc ggtgatgcca gtcccgcctg gtccccggac ggccgctcga tcgcattcct
     3721 gcgcgcgaca ggtgatgggg ccgaggtcct cttgattcct gcgctgggtg gcccggagcg
     3781 taaagtggct gaagtactac gtcggcctca gagaccctcg attcctcctt tgctagcctg
     3841 gttaccgcac ggcgacgggc tcgtttttgc agacgcgagt tccgccgatg aatcaactgg
     3901 cttgtttctg ttttcgatga agacgggcga gaggagacgg ttgacgtccc cgccgaaagc
     3961 atccgtaggt gacagcggcg tcgccatctc gccggatgga gagaagctgg tatttgtccg
     4021 gactttctct tttcaaaaca gccatctcta tctattagcg ctgtctagtg acttgcggcc
     4081 agttggtgaa ccagagcgga tcgccaccaa tctacaggct tgttacaatc cagcttggac
     4141 gtccgacgga aaggacattg tgttcagtgc tgttgagcaa ggcaacgaac ggctctggcg
     4201 aattcctgca tccggctctg cggttgcccg gccactggcc gccggggagg gtggagacga
     4261 cccggcgatt tcgcgacagg gaaaccgcct cgcgtaccgc gtaggtgcca cggactcaaa
     4321 tctctggaaa gtggcaattt ccgggcaggg aacgaatcat ccagtgaagg tgatttcatc
     4381 cagtcgccag gatgtgaatg cgcagtactc cccggatggg aagcggattg tttttgcctc
     4441 tgaccgatcg ggagacatgg aaatctgggc tagtaatgcc gatggctccg cccctgcgca
     4501 actcacttcg cttggggcgc actccgggac accacgatgg gcgccggacg gccgacggat
     4561 cgcatttgac tccaacaagg aaggacgctt tcaaatctac tccatcgacg ccggcggggg
     4621 agttccacaa cggctgacca ggagtcctgt agatgatgca gcccccagtt ggtcacggga
     4681 cgggaaacag atctacttct cctcgaaacg aacgggccgc tgggaggtgt ggaagatgcc
     4741 cgccgaaggg ggagacccgg ttcaggtgac tcgccagggc ggattctctc ccttcgaatc
     4801 cccagaccgc cagttggtct actgtcaaaa aacggagggc aactcggagg tttggaaagt
     4861 gccggtggac ggtggccagg agagcaaagt cctggacccg gtcggaggcc gacagttcgc
     4921 ggttgccgat caaggaatct acttcatcag gtaccgggcc gcaacgccga ccctgcaagt
     4981 ctttcgtttt gcgacggggg agacgaaaga aatcaccagg ataggaaccc cgaccatgat
     5041 gggcctgagt gtttcgcctg acggccgatc attgctttac acacaaatag atcaggtcgg
     5101 cagtgacctg atgctggtgg agaacttccg gtagcgccgg ataggtgaag caagcgccgc
     5161 tttgtctagc gatctggaag tgtgtagcgc gcccagtggt ggcggcgaag gagggtttag
     5221 cttcgatgag ttgttcctcc cgtgaggctc atcgtgacca atctggagac cgatgagccg
     5281 cgtggtagtg agggcttaca acaagcgggg aacagcggag cagtggatca gggaggcaat
     5341 caggacggtg aagatggtgc gactgagccg gttgggtgca tactctgagc caaaaacgcc
     5401 tcatcggatt attgagacga ttctgcggcc taacaatgaa cggctttcga aacgagaggg
     5461 acaaatgagc acacgagcag cactacttgc acagcggatc ggacaaggcg ccgacacgct
     5521 tgcggcattt gcagaggggc tgtcggatgc gcagtggcac acggtggtgc cgcgcgacgg
     5581 tcgtcaggtc ggcgtgatca ttcaccatgt ggcgagcatc tacccgatcg agatcgatct
     5641 cgcccagcag gtcgcctccg ggaacaacat tgcgggagtg acgtggggct tcgttgccga
     5701 gttgaatgcg aaacacgcgc acgaccatgc cgcagtcggg aggcaggaaa ccatcgatct
     5761 tctgcgcagc aacggccatt ccgccgcgga ggcggtgcgc gccttctccg acgaagagct
     5821 cgaccgggcg gcgcccgtct cgctgaacgc agacgccccg ctcacggcgc aatttctcat
     5881 cgaggaccac gcgctcagac acagctggca ccatctggcc aaaatcaagg cggtcctcgg
     5941 gagtgtttga cccacgggcc catggcgtgg tacctgacct tcatgctcaa acgtggcggt
     6001 tatcgccgct gtcagcttac cgtctatctg cgtctgaagg gaaccgaaat ttccagtatc
     6061 gacggcgcca gcgggaacag cgagtaggcg aagggcgcca ctggttttgg cacgagtgaa
     6121 tcaactcgga gtccggactg tcagccgacg gtttgcgcga gagcgtccgc tgcgttctgg
     6181 cctcggctgg ggaggcttcg ttgaaccggg gcgtggattc atcatcttcc gtgagcagcg
     6241 gaggaccggc agacgagaag caccgcacgt gctggaccag cggatagctc ccgaaaacgc
     6301 ccgtagtcgt cacctatcgg ggttggttgg ccacatctgc ctactgctac tcagcccgag
     6361 agccgccacc gctgctgtca agtagtaaaa ggagccattg ggcaggcaga aaaagcactg
     6421 cgcgacgtca agcagttatc tcgcgcatgg cgcgtgccgt ctcttccgga cacgcggtgt
     6481 tgattcgcat gccggaaccg atttcgaacc cggcgattcg tgtcaggcgg ggcacgattt
     6541 cgaggtgcca caaaaagtag tcatcatcct cgccgtccac gggcgctgag tggatcacgt
     6601 agttgtagtc cgggtcgttc aggagctttg cgagccgcga gagcgactcc ttcaagacgg
     6661 tcgcgaggtc ccgcaattcg tcgatggtcg cctggccaaa agagggccgg tgccgctttg
     6721 gaacgatcca agttccaaag gggacctgag atgcaaacgg gtggaagacc aggaagcagt
     6781 ctgtctccaa aatgacgcgc tggccggacc gcacctccgc cttcaggata tccacgtaaa
     6841 ggcaacgccc cgtggcgtcg taataacgaa tcgcctcctc gcacttcctt cgcacgtacg
     6901 ccggcaccac gggggtagca acaatctgtg agtgcggatg ggtgagcgag gtaccggcgt
     6961 tctccccgtg gttcttgaat atgagaatca gcttcaaccg ccggtcccgg cgtaggccga
     7021 ggtagcggtc ccgataagcg tgaaggattt cttccacttc ggcaggactc atcctgtgga
     7081 tgcagcggtt gtgaaccgga gtctcgatca cgacctcgtg gtatccgaca ccttccatct
     7141 gcaggaagaa gccgtcgagc agcagacgtt ccgtgtttcc ttgaggcacc agagcggcga
     7201 acttgttggg aatgacgcgg acagtccagc caggctcgtt ggcggcgcct gtattccgcc
     7261 gcgccatcaa ctccggtggt gtctgacgtt ccagcccagg gcagaaaggg cagtttggat
     7321 cgtattcagg ctgcgcgggc cgagcggcgg cacgccggaa ttcatgaggc cgctgcatcc
     7381 gctcggtggc gatgatcacc cactccttgg tggctggatc ttgtctcagt tcgggcactg
     7441 ctatactcct cttgtttccc caaacgggtt tcccggccga cgctctgcaa cgggcagaat
     7501 tcgtcagccg gctgggccgg ctggcccgcc caacatcagg aagcgattgt agagccagcc
     7561 gaacagaaat cctgcagctg cgccatccag agttccccag atcgtgcctg caagcacgct
     7621 cacaactgtg ggcgctgcgc cgtaacccgg ataaacggaa ctcatgagac gcaggaactc
     7681 aacgccataa ggtgaaaaca cggcgttcag caatccgacg aacagcatgc agccgcccca
     7741 cagggcagcg aatgccagtg cacaagcttt tgtcgatagc ttcatttttc ttgcgccatt
     7801 cttccttctg cccgagccag gagggcgcgg gcacacttac gtgatggccg gagttggtcg
     7861 ccagcctgga tctagctgaa ctctaagccg caaaaggtct gactgacgac cacaagaccg
     7921 tcaacgggag cgcgaaccac caccttcccg acgttcgcct ccgctcgtac gctcctcgac
     7981 ccgtgcttcc tgcatcgtca ggctccgggc aaagagcata tcgtctcgat cctttggccg
     8041 ggcttgtact cctttcgttc cacaggcggg caattagcct ccggagcgat atcccgccta
     8101 aaagctgtcg gagccttgat tgggcctctc gcagttgtct gtcgagttct tgcgcgtggt
     8161 tctcaaaccg gccggtacaa tgtttcctgg cctgatcgag atactcccac atccgctcga
     8221 gctgggccgc gctaaactcg agcttcggag ctttcttaga gagcaccgtt ccatcccgat
     8281 agagccgacc ggaccaccca tgacacttga tccgcctcta gcggtttcgg cagcagatcg
     8341 tacccgccga gattaaggac ttcggcccac atagtgtcat ccctcagccg ggagacgaca
     8401 atgaatttcg gcgatagggc gccttcaacc accgagatct gttcgagcag ttcgctccag
     8461 gaaggagctt gcgcgtcgcc atccatgatc acgacaggaa tggagtcggt agccggcacc
     8521 ttacgcgcct cgatcccgtc tttagctaag cgaagtctcc gctgtgaggc ttcgagcgct
     8581 gccgtcatga agagccggcc actctcatcc gctgtaatca ccagtgcgac cacaccgtcg
     8641 tggtcaggaa gcgacggccg cgccatgatc ggtcgcttct cgcttaccat ttccgtctcc
     8701 tcgcgctgcc taaccggcaa gggcgcacgg ggtctgatac tccgcgagtt gctcgttgcc
     8761 ttcggattgt tccgcacgtt cctggcaaga aacacagtaa ggagaccaag gaatgattgc
     8821 gagtcgtctc gtcgggatgt cccccccgca cgacgcacaa atgccgaagg ttccttcgcg
     8881 aatctcgcgc agtgctccct cgaccgagcg aagctgcgcg cagattcgat cgacattgcg
     8941 aaggaggagt tcgcgatccg caatgctgcg tacctggtcc atgggatcgc tcacggggtc
     9001 gatggctagg cgttcgcgcc gttcacaaat ctctcggatc agctcgtcgc gcttggcctc
     9061 gatggctcgt ttgaatcggt tctcttgagc tctggtcatt tcggcctcct gttgtgtcac
     9121 caaccttgat tctaggaatc ttgtgcgcgg cggcgtatcc cctggacaag tgaagtcacc
     9181 tgtaaattgt ggtgaggtgg gacagggagt gggcgagcgg atttgacaca cactccatct
     9241 tcttgtgcgg actgcccgct catgctgtgc ccgcgcgcag gccccgctac tcctcgagca
     9301 gtagcctccg ggtggccggc gacggcacac cgctgaccgt caggttgttt gccttctgga
     9361 actcgccgat cgccttcatc gtcggctcgt caagtccgcc attcacatcg ccctgatata
     9421 gcccgttctt tttcaacacc gcctggacct gctcgatcac accggggttg tagggctcgt
     9481 aagtccctct atccagtcct gtgatcaggg ccacgctctc ggtttccttt tgatcctgga
     9541 ccgctaacgc cggcagcgcc acgaggaggc acgccagcat gagagcgatt gccagcacga
     9601 tccatcggtt ctttccatac ctccttgccg tgctcacttg tgcatggccc ttgtgcgcat
     9661 aaggtttgcc tcctaccacc agactagtgg ggtgcaacgg gaaagggtat ccctccaatg
     9721 cactaactgc cggccgacat ctcattagca gcgtctcgcc tgtatcaaca tcaagttgtt
     9781 cgagagtaac tacttgccag cagcggcacc aaaaccgttc aacgcgccct cggtcttgta
     9841 ccaggcctcc tccggtgaac cttcctggcc accgcgcgcc tcccagtagg aataagcgag
     9901 gcggacgatt tcctcatggt caggattgga gttttcagca gcgggcgtga atccgcaaaa
     9961 gatcatgcca ccttgcgggg cggcggaact tcagaaagct tctgtctcca ggcaagccaa
    10021 gcggaggtgc tgactcgcaa cgcctcgtct gggtcaaaag gtgtaagcag cacgtcataa
    10081 ccgccgaggt tcaacacttg tgcccagagc ctgtcgtccg ccaggcgaga tgagacgatc
    10141 aggttgggcg ggggtatgaa acctgtcaac gcaccgagca ggtccgtcca gcagccgtcc
    10201 ggcagctctg tgttcgaaat cacgacgccg acgggatgct ctcgcagaaa ggcgagtgcc
    10261 gcctggcacg tcgagaacca gtagacgctt gatcgtgaat ggccgaaaag gcgccgaaga
    10321 ggcagactat ccgtttcgga gggtgagatg agaacaactt caatccccgt ctcaaggacg
    10381 gaggaaccca gagagggcag catgttattg ctcctcgctc acccctgatt ctgtatctga
    10441 cgaaggacca tgtcaagggg ctgtttcagg tctttaacta ttcaaccgcc tccaggtttc
    10501 agaacgtatg ggcaccgcgg aaccaaagcc gggaaccgtc agggctccag ccgacatcca
    10561 ttccgagcag aaagctctcg cccagattct ttaaaggaat gagccggaca cgctgccgat
    10621 tatttcgaaa tcgagtctat cgtttgaaga agaattcgta agccttattc tccactgacc
    10681 ggtcccttat cgttaagacc gtggttaaca ttcttccctc cgcccccaac gtgaagcggc
    10741 ggctacgcga gttttcggga cagctcacct cgacgaccag cctctgcccg cgccaccagt
    10801 gccatcgcgc actcgccgcg catgtctgtc cctttaacgt gtaagcatgc tgcttgccat
    10861 cggtggttag ctccagcgtc tcgttcgttt cccctgtctc gttggtggta acggtgaaaa
    10921 ttcgaatctg gggttcgttg tgctcgatct ggatcgtcag actcttaggc ggtggaacat
    10981 tcccgaaccg gcttctctga agatccaact gccacgtccc gctgaaatca ggcttgttcg
    11041 acttctgggc ggacaacccg agagctataa cggccaacat cgcccacaat cgcagtgcga
    11101 cactcattgc agacctcctg ttcgcgactc gtgacgtttc ccgccctgac cgatgactgg
    11161 cgggcttccc tgccgccccg agtacttgtc cagtgacgat atcaatcttc tggcgagccc
    11221 cgggacctgc aattctttct cgatgatttc acggtcttcg agcgtcctgc cgtagagctc
    11281 gccgttgtca ctcacgtcct gccgcaaggt ggtgccctcc aaggatacgc ctgcgaacac
    11341 accggctgcg acggaggtgc cgacgcccaa catgaactgg ctggagagca ggcgctccat
    11401 cccgcgctca ttcatcgcca gcagaatgac atcggtttcc gagccgccta cttgcggacc
    11461 gaagctcacg ccctcaatcc gcacagtacc tggagcggtc caaccgaccc ggctcttatt
    11521 cctacacgaa acgtagcctt tgccgtagcg agcgccgaag attaggaaac cccgttttag
    11581 ccctggcacg atgacggagc aatgtgcacg gtccagcagt tcctgtggga ttgcccggtc
    11641 gggcgtctcc atgacttcct gaaacacggt cgttgcgtcg cgaagacact cgtcgtgttc
    11701 tgacgccgcc aagcaagccg caaccgaaga tcgaaagaac cggccacctc atttctacct
    11761 ctgttccatc gatctggatt ccggcgacgg cctggaatcc ttttcgccct tgggaactct
    11821 cgccaccatg gcaatgacga acacgataac cgttccgccg gccagcaaca ccgcaccaag
    11881 aataggtggc agtgtgatcg tccagttcga ttgttcagta gctgcctgaa gggggccgac
    11941 gtgcaatagt tgccgctccg ccgtggtgag ggtgattccc tgataggtca gcacggcggt
    12001 accggccaca atgagcacga tccctagaaa gagactgagt atggtcttca tcaattcctc
    12061 caagcgtctc tcgaggcctc gccagcgtat cgagccccga aagtacatgg gcctgcattc
    12121 ataagcattg tgcggcgacg cgggcgggca ctccatccgt tgggggcatg agtttcggac
    12181 ggttcaggcc gtaaaatggc tcgcccttct gcacggcatc gaagaccgcc gcgggaggac
    12241 gcgcttctca cgctaggggc gaaactctgc ggccagaaaa attgacttct gaagaatgca
    12301 gatgcgtggt ccgaagcttg acactcgtta cggatctagt caacagccag cgataccatg
    12361 tacacaaggc cggcaacgaa gaccgcaata agagacgccc aaacccatcg gaacaccggc
    12421 cccaacccgc ccgatgcgtt tcccagcgag ttgtgcagat cgcctatcag catgtttggc
    12481 tcctcaattt cagtctatga acgacgggtt tgcgcggaca tcccgcaaac aggtgacatg
    12541 gtcccctgac ttccacttca tcgtacgggg gtcttccgcg cgtgttcact ctcggccgtg
    12601 ttcgtcaaca ccgccaccgg aaatcggcgc aacagatcgc ggactctcag gacgctgcgc
    12661 acgccctccc ggagctgaac gtcgactatt tcgccggaga ggacatcacg ccaggagtgc
    12721 ggagctccct ctggcaaccg cagcgcggta tcggaccatg cccgttctcc gagagggacc
    12781 ctgtatgttg cagtcaactg cgttgtccat cgcggagcga cggtcagagc ccaagtggtc
    12841 tcatggcgcc ggcagaacgc cacgaggttg tcctttttgg ctcccgttgc ttccactgga
    12901 aggtagtccc cggcgaggaa gacatccgtg tgagcgcggc ggaagtccaa agccttgtct
    12961 gtaaggtaga gtttgatacg gccgtctttc cagccggaga ctatctccct gagcagtccg
    13021 cgcaggttct ctgtctcccg cttccgcagt tgctccagca gcgcgatacg cgtccgatag
    13081 tccacggggc gccgattgtc gggatccacc agactgaaat cccagagctc cgtgccctgg
    13141 tagaaatccg gcacacccgg cgctgtgatc ttaatcagca cctgcgacag cgcgttgaaa
    13201 gcgccatgaa gcgctaaccg ctcctgaaat cggaggaagt ccggcaggaa tcggttggat
    13261 tctgtggggt ccagaatgct ctcagcaaaa caaacgaggg cttcttcgtg tgcctggttg
    13321 gggcgaagcc aaccagagtg gtccttggct tcgcgcgctg cctttaccag gaagcacttc
    13381 agacgttcca ggaaactccg gttctcgtct ggctcaaggg gccacgcgcc tagtagtgtt
    13441 tggtagatga gcacctcctc ggtcggggag ggaacctcat ggccgttcac ggtggtcttc
    13501 ttgtcgccgt tccaacggtt ccagcgcgag aggcgatttc tccattcgtc gggcagctcg
    13561 ctcagcacgt tgattctggc cctgacatcc tcgctgcgtt tggtgtcgtg actggaagtc
    13621 gtgttcatgg cgtacggcca gtgctcattc tggtccttga gaatgctgtg gaagtccgtc
    13681 agagaatatg gcggtccctc acgcagagga tcacctccca cttcattgcg cgagatcagg
    13741 cagtgctgcg catagaaggc cgtgtcttcc aatcctttcg ccatgaccgg gccggtgaac
    13801 tgttgccacc tcatgacgaa acgcaaccac tgcggtttct gatccttggc gtaagccggc
    13861 ggctccagga gcaagacccg ccgaaggaaa tcgaatgcgg ccgtgctgac gcggatctcg
    13921 gaggtccggc ggcgcgcgag gtccagggtt cgctcgatgt agtgccggtc ccgctcgtcc
    13981 acggtgaaat cccggatgta agtccgataa accggaaggc aggcggtagt ctccaccagt
    14041 gcgttcatca actcggagag ggggacatcg cgcgcctgcc ggtcttgtgc cgcaagggcc
    14101 ccgaggtgat gtcccagttc gtgtacctcg ccggcaaaca gcttccacat cacctgcttg
    14161 ttgcgggcat aacagacttc ggcaaagggc atccggtcgc ccgtgaagtt cgcgtacgtt
    14221 tcctccagcg atcgcagccc gtcgggatgc acgaacacgt cgttcaagac gttcagaaag
    14281 tcgtagccag ttgtgccgca ggcacgccac tgtcgcggca gaggttcgtc gcgtccgaga
    14341 accttctcga ccacaacata gaagctcggt ggcgaaccct tgccgccgag ctcggcctgt
    14401 agccgttcca ggtaaccttg cggatcgtga agcccgtcga tgtggtcgac gcgcagaccg
    14461 gtgaccttgc cctccttcac gagttggaga atcggctcgt gcctcgtgcg aaagacctcc
    14521 tcgtcttcga cccgcagacg cacgagatcg ttaatgtcga agaagcggcg gtaattgatc
    14581 tcctcgtacg cgatcttcca atgcgcaagc cgataaccct gtccagcgag tattcggtca
    14641 agaaaatcgg ctctttcggg ccggtccttt gtgtcactga ggtcccgcag tgccgcatcg
    14701 attgcgagac gaacttcgag atgatcacga tagaggtgga acaatcgctg cttgagggat
    14761 tgcgaaagct ctcgtctgcg gccggcctcg accggttcag tcgcagtgcg ccgcggcatc
    14821 ccttcgacaa tggattggag ctgccgcagt tcatcgatcg atggatgacc tgctgcaatc
    14881 tgccctgtga tctggtcaac gcattgttca agaatcggag cgtacgaaca gggatcgagg
    14941 ggaagccggc gctcatggta acgcaggaag aagccggact catccagatg aacggccagc
    15001 ttgccattct ccagcacatt gccgtaaagg tcaccgagca ccggaagcag gatcttgtct
    15061 tccagtagaa aagcggcttt ggacgtggct ggacgccaat caatgtcgaa gaattgcgcg
    15121 tagggagagc cggggccgtt ttcaaggaca tccatccacc aggaattctc atggctggca
    15181 gccatgtgat tgggtacgat gtcgaggaga aggcccatgc cgtagtgctt gagcttctcg
    15241 cacagatcat cgaattcttc atctgtaccg agctctgagt tcacgcgccc cgggtgtgtg
    15301 acgtcgtagc cgtgcgaact ccctcgctgc ggccgaaacc gtggagaaga gtataagtcg
    15361 ccgacgccga gtccatgcag ataagcaacc aactcgcggc aatctacgaa ccggaaatcg
    15421 cgtgtgaatt gaacgcggta ggtcgatatg ggaacacgca gctgactcat ttcgctgcct
    15481 cagcggtacg cggctcaaag taaaaaccga gcttcgctcc cagcgcaccc atggtccgaa
    15541 cccattcttg cgccgcgctg tcgcatccgt tggctttcgc ctgcaactcc tcgaatcggg
    15601 cctcccgaac cgcgtgatag acattttgcg ccgcccagag gttgacctgg aacggcagga
    15661 gcgggcacag gctgacgact ttgtggaagc gttcgagcaa ctgccgctcg tccggattgc
    15721 gatggaaagc ctcggccgcg tgttcgaccg tctttcgaaa tgcaaactcc agtgtgacca
    15781 tgtccagaag gatccctgta cggttcacct ggtccaggag agcctgcact tgattcgtct
    15841 caggttcatc cgtcgataga gctgccgaca aatcctcgtg cagtgtgaag tcgaccgcca
    15901 tctgaaaacg cgcgggcagg gggatgccga gctccgtaac aaagcgggcg agggtggcgt
    15961 agttgctgta gaaaccgcgg tacaagaagg ccgcctcagc caacgctttt tccatgatta
    16021 gacccacgat tttccgctgc tcgtcccgga acagcagtcg cagggaatac gtgccactgc
    16081 cgaagttttg gtcgacggaa cgaattagtt ccggaatttc gccgcggtgg aagatctctt
    16141 gaatttcggc taggagagcg ccgtaggcgt cggctcctct atactccctc acgccgccac
    16201 tgacgttctg atcaccgagg tggatcgctc cgaaactaag tgttgtcgat tcctcggtga
    16261 tgcgggaagt aatcttggcc cgtcctagta caagatgcgc tttcccctcg tgcactcttt
    16321 ggtagtcttc ccgatccacc tcgtaggaat agatcccgct cttcggcggg tactcctcaa
    16381 acagtgaact gatggcataa tgagccccga ccttctccag attcaccatc gcgggtttca
    16441 cgaacttctg gtaaatcaat ctgccgtcgc ggtgttccgg caggttgctc ttggctctct
    16501 caaggagggt cagaaagcca tcttcgagtc cgtcgccgaa catctcctgg ccaagctgta
    16561 atgcgcgggc ggcgtattgg atcacctgca cagtctcgat gccggacaac tcgtcgaaga
    16621 accaaccaca actcgtgtac atcaacatcg cgtgccgctg gagctccagc aacttcagcg
    16681 cgcggactcg atccgccggc tggagcgagc ggttggcatg agtttccagg aagcggctta
    16741 cattatccgg cgaccgatcc agaatgacgt caatgtaggc atcgcgcgcc ttccaagggt
    16801 tgcggaaaac ctcgtgccca gcttccttga agcgctccgc cagttggtcc cgcagccagt
    16861 cgagggcctg gcgtagcggg gcacgccagt tctggtgcca tccgggatgc gccccggagt
    16921 tgcagccgca gtctgccttc cagcgctcga cgccgtgggc gcagctccag gaagtactct
    16981 caataacctg cacctcgtgt gacggagggt acctctccag aaactcgctg tagttcgtca
    17041 atcgagccag actacttgcc tcgatgaagt gaagcgcgta agcgagcgcc atgtctccat
    17101 gccggcgatg gtggccgtaa gtttcgccat cagtggcgat gtggaccaac tgcggccagg
    17161 agcgggaatc agcgaacgct ccattcaacc gctgcgccag gttctcgccc cgaaccagga
    17221 ggtcctcgaa agccaccgct ttcgagatcg ggccgtcata gaaaaacagc gctaatgaac
    17281 ggcgtgacgg caggcggatc cggtaggcga tcgaaggatc gatcctgccg ccgttcacat
    17341 ccttccagac gcggcttcct attggtcgca ctttattcgc ctggtacggc gaaaggaccg
    17401 taaacctgat tcccatctcg gcgagaatgt ccagcgtctc cagatccacg gctgtttccg
    17461 gcagccacag gccttccggc ttgcggcgga accgatgccc gaagtcgcgg atgccccaca
    17521 gtacctgcgt ccgcttgtcc ctattgttcg ccaggggaag aatcacgtga ttgtaggctt
    17581 gagccatcgc cgaaccatgg ccagagaatc tccgctgact ttcgttatcg gccgcgagga
    17641 tggcgcgata gacatccgga cattcctgct ccatccaggc cagcagcgtg ggaccgaagt
    17701 tgaagctgat ccttgagtag ttgttgacga tctgccggat tcggttatcg gcatccagga
    17761 tccgcgaagc gctgttcggc gcgtagcact ccgccgcaat ccgttggttc cagtcgtggt
    17821 acgggtatgc cgaatcctgg agctcgatcg cctccagcca gggattctct ctcgggggct
    17881 ggtagaaatg cccgtgaatg cagacgtagc gttccatcta tgcctccaca caccgtctgt
    17941 ataacaccgc cgatcgccgt ttcaatgcga gttccatgtc gttaggcgaa tccagctctt
    18001 ccggtagatc ggagccgggg ccaaaccagg ttgcatctga cgagtccagt agtttcgacc
    18061 aacgcccggt cgccacgggc agcttcactc gaacgccagt ttcagcaaaa cacagcacca
    18121 gaatcacttc gtcgttgtcg cgccatcgcc tgacgaatat cactttggcc tcttcatccg
    18181 tcaccacgga catttgctct ctgctgagga aagccaatgc cgggacctcc cggcgaagat
    18241 gtaacaacgc ccggtagaaa cctaggagcg tcccgtgctc acccttctgg cggagttcga
    18301 ggtttagctt cgaccgcagg aaggtcccct ctgcctgagg atccggggga tcgccctgcc
    18361 aagcgaaggc ggcgaactcc tccttccttc cccggcgaac cgcttccacg agattcgcat
    18421 ccgagtggct tacgaaatac agaaacggcg cagtctcagc atactcctca cccatgaaca
    18481 aaagcggaac gtggggtgag agaatcacgg ccgccgccgc caatttcagg tcttcgaacg
    18541 acaccaggtt gccgagacgc tcgcccagca tgcggttccc cacctggtcg tgattctggg
    18601 agaacaccac gaacttgtag gccgggatgt caagcgcaga ggcgccgtgg cggcgccggc
    18661 ggaaccgaga gtattcgcca gagtaaacgt agccttcttc gaacgccttc gcgatatggc
    18721 gcagttcgcc gaagtcctcg tagtatccgg cccgatcgtc ggtcagcaga actcgcaagg
    18781 cgtggtggaa gccatcggcc cactgggcgt ccaggccaaa accgccgaga tccttggacc
    18841 ggatgagacg aggatcattc agatcgcttt ccgggatcac gtgaacccgt cggttcaaac
    18901 gggtggcctc ttcgcgaatg gcgtcgccca attcctccag aaacgggtag gcagagcgat
    18961 cgaaaatacc gtgcacggca tcgagcctta gagcgtcgat atggaattcc cggacccaat
    19021 aaagcgcgtt ctcgatgaag aaccgcctca cttcgtcgct cccgggaccg tcgaagttga
    19081 tcgcaggtcc ccatggtgtc ttgtagttct ccgtgaagta cgggccaaaa tccgaaaggt
    19141 agttgccttc ggggccgagg tggttgtaaa cgacgtccag taccacgccc aagccgtgct
    19201 cgtgacactc attcacaagc cgcttcagcc cctgcgggcc accataggag ttctgcaccg
    19261 cgaagggata ggcgccatcg taaccccagt tccgctcgcc tggaaactgg gcgaccggca
    19321 tgagctcaac gcaggtgacg ccgaggtcgc gaaggtattc gaggcaaggg atgatagcat
    19381 cgaaggtacc ctccggcgta aatgctccga cgtggagttc atagaggaca cactgctcga
    19441 tgggcagccc ccaccacgct cggtcctgcc agtgaaactc cgcattcaca acctcggatg
    19501 gtccgtgcac tccgtccggc tggaaccgtg aggcggggtc cggccggttc agtcgcccgt
    19561 tgattcggta gagatagcgc gtccctggag gagcgttccc aggaactact tcccagtagc
    19621 ccgccccgag ctggtgcatg gccaccgcga agcgctgcgg ataagtgatc cgcagttcga
    19681 cgtccgatgc ctgcggcgcc cacacgcgga aacgcgtcac gccttcgccg aggtggagag
    19741 ttccgtgtcg gaacccaaag tccattggtt ccatgcagtt gtttctttta aggttagcgg
    19801 gaagatgggc aggttagaca tcccaccaac acaggagggt ttggtctttt atgccgcagt
    19861 ttgccgcccc tcattgagcg ggactgagaa agcgcatgcc ggccggtcct gcaaggccgc
    19921 agcgtgttcc aagactcttc tcgcgtgctc ggcgatcata cgaaactccg gcttcgtatt
    19981 gcaggtttgg gttgcctgga ccaccgttgt aggcccccac cgctcgcgct acgtcgccct
    20041 ggaaacgggc gaggagatcg cgaagaaaaa aacccgcaaa ggtggtggcg gcttccggcg
    20101 gcacatcgtg gagattgagt ttctttggag gacgcgagtg ctccgctagg gtggagtagt
    20161 cgtgcgcgtc cttctgggag aggccatgtg cctagcgcaa cgtcttcctg gtgattcgcc
    20221 agatgccgga ggattcgtcg aaaccagaaa ccctttcgac cgcgctttca ggatcacatc
    20281 gcctttctca gcgtgcctca ggacatgctc caggcgcaca gtaggacggc gacgacgccg
    20341 actaccgccg gccaggtacg catatgcgcc tcctggtccg tatttgccaa cgtaagcgga
    20401 gaccgcgtga aggcctgaca ggaaatgatc tctcgatcta caattcaaaa gggatggaac
    20461 cgcagagccc cgccttccga acccgcattg agcatctttt ggccatcgac cgggagtcga
    20521 agccgaaagt ctacttgcag gtcttcgatg ccgcggaggt cttcagcctc aactacgcct
    20581 tggaactcct cctgtcagcc ggcatcgcca cgcttggctt ggtgttagac agcccagcgg
    20641 tcgtcatcgg cgcgatgcta atttccccaa tgatgggccc gatccttgcc ggaggcctgg
    20701 cacttgctgc agcggatatc tatctgggga tcaagtccct gctgagcgtg gtgctgagtt
    20761 cgctgtgcgc gatcctgttc gccgccgcgc tcgtttggct gctgccgttc cactcgccga
    20821 cccaggaaat tctcgctcgt acgcatccaa acctgctcga cctggttgtg gcgctttttt
    20881 ccgggctcgc cggttccgtc gtggtgtgcc gtggcggggg tggtggcggc gtcaccgcac
    20941 tgcccggtgt ggctatcgcg gtggcgctga tgccgcccct gtgcacggtc ggatttggag
    21001 tcggcagcgg attctcttgg ccgatcatca ccggcgccgg attgctgttc ttgaccaacc
    21061 tggctgccat catggcgagc gccttcgtca cgttctatgc ggtcagaatg gacgcaccag
    21121 agctccgcat gaagatcgat tattcgatcc tggagcacgc ggcctcggac cgtctctacc
    21181 gcctgttgat gaagacccgc cttgcccgca cgttcggtga catcggcaag ttgcgatggc
    21241 gcgtcctgat gctggtcgcc actcttgttg cgttgttcat tccgctgcgg cagtccttat
    21301 ttcagttgag agaagagacg attgcccgga cagccgcgaa cgaggccgtg cgactgatgg
    21361 cgtccccggg gatgatcttc acccaacaac tggatgtgac tccggagcgg atcatccttc
    21421 gaatgatcgt gacggaaact gtgcccgagg acaggattcg agaagcggag aaactcctgc
    21481 ttcgccgcac tgggaaggag gttactgtgg ccgtccgcaa ggtggccagt gaggaggaac
    21541 tggccatgct ccgcgagcgg tttcgcaccc cgccggcgcc acctcagcca cgagatctga
    21601 attccatgcg gaccgaattg atcggccgtg ttgaacagcc cctcaaggag gtctggccca
    21661 ccgagttggc gccactcgtt tcctacgaag tcggattcac tccagaagat attctggtgc
    21721 gagtgcggta caaatcagaa accccgctcg acgccaccgc ggaggcaatg ctcaccaaag
    21781 tgctccagac ccggttgaat gagccaaagc tgcggctcat cttggagcat gaagcgactc
    21841 cgaagtccag aacacgcagg agcgtcagga agtagtccgc caagcagtct gggcggatgc
    21901 gcggtgctcc gcgggttgct gtggtggctt actggcaatt ctcggcgtgg gtctgctcgc
    21961 cgcctaggac gtgcttgaca cgaacctgac cttgcagatc atcgaaagta ctgtttttct
    22021 gttcccaaag ctgctggccg aggtggtcat cctagtcacc ggattttggc tggtgcagta
    22081 tatgagcggc agcatggtag tctaggccgt taatgagaac cttccgttcg tgtctcttac
    22141 ggtaacggaa acgccgagcc ggagaacagg agaaattctt gtggaaccac ctttgagtcg
    22201 cgccagctag agtgccaagg tcagagtgcc cgtcgcaacc aaggcgatcg cccagacccg
    22261 atgcgcggcg tagcagcgcg acaccaaact tctcaaagac cagcgcagca actgccgccg
    22321 tcaccagcag gtatccggcc gcgtgcgcgc ctgtggccag aaatgctaca ggcgcactgg
    22381 aggcgctcat gctgtgatgc gcgttccgtg aaaggccccc aatccgatga gggccaccca
    22441 tgaggcaact gcgtggtctg tcatccgggt cgcctcatga ataacagtaa gagtcggagg
    22501 acgcgtcgcc gccctccaac cggacctggt gcgcacgcat gctgccgaac tgaaggaaga
    22561 actttggatc cagcttcatc cctcctcctt cgagcacatc gatcttggcc atccagccga
    22621 gtatgcacac tcttcatgct tcatgagcag cactgctgac cagcggaatg gcccggctcc
    22681 tgaggatatg gagtgccggt tgtgcctcgg tgaccgagta ggtcgaaatg cttcaggagt
    22741 gtgccatcga ggtcggtgag aacgacgtcc atctgccacc ggagtacacg aactgtccgc
    22801 tcgtagcggt tttccgactc tgccagacca caagtgactt aaaatacagg cgttatgaga
    22861 atgcgaagct ggccgttctt acttatggga tttggcgccc tgctggtact gattgggctg
    22921 tcctcggcgg ctttgcatca ccgcatggaa caggttcgcg cggaagtgca ggcgcttcag
    22981 caagcgaacg aggaaaccag ccgagtgctt aatgatttgc gatctgaaat ctacctcttt
    23041 gcggtcctgc tccgggatta cttgctggag cgttccccgg aggccgccgc ccaacaacgg
    23101 caggccctgg gggaacttcg caattctacg caggatcact tgcgagcgtt gcaggggaag
    23161 gcggtgtcga gagatcggcc cgaggtacaa cgattgcgga atgtcatcga ggaatactgg
    23221 cggtcggcgg agcctgtctt cgggtggacg ccggcgcaaa aggcgtcaga aggctcagtg
    23281 tttcttcggc ggcgagtcgt tcctcagcgg caggcggcct tccagctggc ttctgaaatc
    23341 gagaatgtga gtctgtcaca atcacggctc cgtcagcagg aagttctgcg tacccaggaa
    23401 ggtctgaaag gctacctgag gcgcgtcgct gccgcggcgc tcatcctcgg cgcggtggtc
    23461 gcgatggtca gtgtcgcgag aactcgctcg ctcgaagcca gcgccactcg gcaccttcgt
    23521 ctgatcgaac gcggcgctga ggacctcagg cgcttgtcac tgaggctgtc caaggctcag
    23581 gaggacgagc gaaaaagcat ttcacgagag ctgcatgacc aagtgggaca gatgctcaca
    23641 gctttgcgca tagaactcgt caacatcgag gagttccgcc aagacgctgg cggcggtttt
    23701 gcgcagcaca tggccgacgc gaggaggctc gccgaggaaa cgttgcggac cgttcgggac
    23761 atctcgatgg ggctgcgtcc ttcagtgctg gacgaactgg gtttgggccc cgccctcaag
    23821 tggcaggggc gcgagttcac gcgtcggagt ggcgtagcag tggagatcca gatagacggt
    23881 agcctcgaca aactcccgga cacgcatcgg acgtgtgtgt atcgagtggt gcaagaggcg
    23941 ctaaccaatt gcgcacgtca tgcccgcgcc agtctgatca ggattacggt gcatgggggt
    24001 gctaccgcga tcgctctgac agtcgaagac aatggcgtcg gctacaacgt tccagcggat
    24061 agctcgcgcg gactgggact actcggtgcc gaggagcgag tgcgagaatt agggggcagg
    24121 atggagatct tctcacgacc ctcaaaagga acgttgctgc gttgcgagat tccaacctca
    24181 ccggaagtga aagtatgacg acgaagacaa agatcctagt ggccgatgat cacggaattg
    24241 tgagacatgg tctgcgttcc cttttggaac ggcagcaaga catggaggtc gtcggtgaag
    24301 ccagggacgg cagagaagcc gtccgcatgg cgaaggaact cgatcctcat atcatcgtca
    24361 tggacatcgc gatgccgcag ctaaatggaa ttgacgcggc ggagcaggtg ctccggacaa
    24421 gtcctccgac gaagatcatc ctcctgagca tgtatgcgga tgaagagttt ttgacgcgag
    24481 cgttgacggt tggcgtgaag ggttacctgt tgaaagattc ggccgaactc gacctcgtgc
    24541 gcgccgttcg tgctgtggcc gccggcaagt ccttcttcag tccagccatc gcccaggcct
    24601 tggctgagga ttacgtacgc cagttacaga gcaaggggct tcaggactct ttcgagctgt
    24661 tgacctcgcg agagaaggag atcatccaac ttttagccga agggaaatcg aacaaagagg
    24721 tagccacggt tctcaatgtc agcccgtaca ctgttgagac acaccggatg cacctcatgc
    24781 aaaaactgaa cctgcacaac acggccgaga tcgttctata cgcggtgagg aagaagatca
    24841 tcacgtgagg cggtgtcggg ccggcttgga aacttcaatc acctgcaggc ccaactagcg
    24901 tcggctgacg cacggttccg gtcgaatgcc gggtgccaag tcctccccca agccactccg
    24961 cagagcgcca aaatcgggcc ctcaacaggg accagcacca gccgccggtg gcgtactggc
    25021 accggccgca aggccaaaaa agacatagtg ggtgacgcgg attgtcttcg cccggtcgaa
    25081 ttggtagcgt gaatacggaa tccgtatcga aggagtaccc atgtcgaatc agccagaaca
    25141 agggaggccc ggtagcacgc ggcggcaatt cgtgggcgcc ggagcagcgg cggttgccgt
    25201 cttgggggcc gcccgcgggt cggctgaaac actggcgatt gacggcggcg ccaagaccgt
    25261 caccgtaccg gacgagcacc aggccgcgat cagcaagtgg cccagatatg gccccgagga
    25321 gaagcaagcg cttgtcgaac tgctcgataa caaccgctcc tacgatgaaa tcccactgtt
    25381 tgagaacgag ctgaaggagt atctgaaggc gccgtacgtt aaggcgcact gcaacgggac
    25441 aagtgcgctg acatcgatgt tcttcgcgct cgacctgcct cgcggcagcg agatcatggc
    25501 tccgtcctac acggcatggg cgacgacggc gccgatgcag tttttcggct acgttcccgt
    25561 atttatcgac atcaacccgc gcaccatgac cttcgatctt gaatacgcca agaagcggtt
    25621 gaatccccgt gtgcgcgcgg tcctgccgat gcactccttc gggaatccct gcgacatgga
    25681 cgatatctgc gagttcggaa ggaaacacgg actggtcatc gtcgaggatg ccgcccaggc
    25741 tcagggcgct tcactgaaag gccggcagat gggaacgtgg ggctcgatcg gagccttcag
    25801 tttccagaac tcgaagatcc tgccggccat tgagggcggt tgcggcgtat accagagccg
    25861 cgagcattat gaacgcgcga ccacgtttgg caattacgag ctgccgcgat cctttccgga
    25921 agacagtccg taccggaaat acggcggcac cgggatggga ctgaagttcc ggattcaccc
    25981 cctggccgcg gcgatcgcgc ggcggcagtt gcgcgggatg gacgagcgca acgcgcgtct
    26041 cgacgcccag gtccggaaac tgaccgaccg cctcatcgaa ctgcccggac tctcgcggcc
    26101 gctgacacga acggacgcca agcgcgttta ctgggcgggg ctgatgcttt tcctcgacga
    26161 cgcgaaggcc aggtgttcgc ggggcaccct gatgaaggcc ctccaggcag agggcgtgcg
    26221 gattgcgcca ggcgcctatg acgaacagca caaatacgtg ctgtattcgg aagcgaagtg
    26281 gtgggaccac ccgcccgtca tcccgtctga tctgcacggc acgaccgagg tgaaccgcct
    26341 ggcggtacgc ctgccgctgt tctatgaaga agcgccggaa ctgatcgatc agtacgtcaa
    26401 ggcgtttgaa aaagtatggg caaagcgctc gcagctggcg agggcgtagt tatcagcggc
    26461 tcaacgagca caggcagagt ctcgaccgga gcggggtagt tcctgggagt ccgtacgccc
    26521 cggtacaggc ggtcgcacca gaagcccttc gatgtcccgg aaagcaacta cacgtcgcgc
    26581 acagatccct ttgtgagcag cggcgccacc aggaaccggt cctcgaacag aaacaggtcc
    26641 cgcgcgacgt acctgttttc gtcttcggaa ttgtggaaga acaagattaa taaaagcatg
    26701 gaagagtgac tcggcttgcc gtccgggtaa ggtggcgctg aagagaagcg ttctccgttg
    26761 cccggggttg gtggcgccta tccgccttgg gccggtgcac atcatcgtcg cgggttcagg
    26821 gccccccctt agttgacggg cctgccgcaa agcgggttct ctcaccggca gttatggccg
    26881 gcaaaccggg ggaccgattc gagcggtccc ccatttacct gcgttttcag tgtggtatac
    26941 cacgccaagc gcttagtgtg gctcagttga catacggcca ctgccttcgc ctcgttaagc
    27001 caatactcct tactgggggc gcccccaatt cggaggtttg tgttgctata acacaaacct
    27061 tgtgttgcta caacacaaac ctcgccgtat agctcaagct cctggcgagt gcggtcaatc
    27121 aaacgatgaa tctccccgaa gcacctcaaa accaagcatt ccgcgagatc caaatcccga
    27181 actcgtggct cttatcgctc gcgtaacacc ttcggaccaa gaagtgctca gtgtttttcc
    27241 gatggcggca aggggcgcgc gtgtcccaaa ttcggagccg aacgacgact cagagaagcg
    27301 cttgcctctc agctatccat ggccgcggtt gcggaactac catacaacgg cggggcggga
    27361 tgaagctgac gatccggcgc gggcgcggta cgttgacctg tgctggcggc ggctgacaca
    27421 ccaacaagtg ggggtactca ggtattagcc aaggcccgac gaggcgcggt agcgggggcg
    27481 gtcctatcgg tctatgaaga agacgattcg acgtggtccc attcgtctgc tgtctcggca
    27541 atgacgctac ggtccctatt tcctggagat gtgcgtctac ctggaggctt atcaacccac
    27601 cgctcaaacg acggagccca tggtggcttc cgtcaacttc taccgcggga aagtcaaggc
    27661 tttcgaaacc gtgccgctcg ggatcataga gggtccgaac tccaggtcca aagcggtgcc
    27721 aatccgcttt cgaatatcga tgggtgacct tcaacccggc agacacactt gccaagtcaa
    27781 tgtaatcaac ccctcgatgc agaagtttgc cgtctggcga gcgtcgaagt tgacgggcct
    27841 cgccatcagc tcaccataca gtttctggcg agcctcgccg ccgaattgaa cggggttgtt
    27901 gatgtcctct tcctggggcc aatcgccggg aataagaaca ccttggtgat agggaaagaa
    27961 ggagtagctg acataaccac cgcggcaggt ccgagtcgcg tgacacgcct ttcgcgagaa
    28021 tccgctaggg aaacctcttg gatcttgtca tctttctcga aaacgaattc gcgattgcct
    28081 tcccggctca tgtgccagtc gacacccgag ctgagatgcc ggacgttaca gccattgtgg
    28141 ttagatctac cgtgaccctc agatctttcg attcaagaac aatgttttgt tgtgcaaaga
    28201 gacgcacgga agtgcatctt tatttcttgg gagcccccgg ttttggagag tagcgaacag
    28261 ggctagacga ctggcgatcc tgcgctcaac acgcaaatcc agttcaggtt ccagaatgac
    28321 gccgggctgc tgggaactcg ggccgcggat taccggctga ggactcccag ttcgcccacg
    28381 cgccgcaaga tctcgccacg gtccatgtcc cggtaagcat ccggcagcag gctctttgac
    28441 gtgcactcga gtaccgcgac catattttgc agctcgattt cggtcgggta ggagggcgcg
    28501 acgaaatcgg cgatggccgc cttcaggtcc tccacgtcca ccgccgcatc gccttcgagc
    28561 gcgctcttca tgcgggcgcg aaccaacaac gcttcggtgt cggcgcccga gagcgtcccg
    28621 gcctgcgcca gaaagaactt ttccgcctcc tccgatgcca cgcgcgtgcc ggttttcttc
    28681 tgcatggcgc gcagcatcgc gagccgctct tcatcggtat gagggtagaa gagggcgagg
    28741 tgctcctctg cgcgtccctg gcgcttcaga tccaccggca gcaaatcggg gcggcaggtg
    28801 agcaggaacc agatcacctt gccccggtat tctgtgtttc ccatgaactg ggcgatctgc
    28861 gcgaacacgc ggctgctgac gccgctgtcc ccgaagttcg aacgagtgcc gagttgcgcg
    28921 tcggcttcgt ctaccaccac tgcgatcgga ctcatggctt tcaatagcgt cagcacgcgc
    28981 tccaggttcg actccgtcac tccctgccac atgctgcgga aattcttcag catcacggcg
    29041 ggaatgccca cttcacccgc gaaacaagtc gtgaggaagg tcttgcccgt gccgaccggg
    29101 ccgcagatca cgtaccccat cggtaacacg tcgaagcgcc ccgcacgcaa cgccgccgcg
    29161 gcatcgcgca gtttcttctt cacggcctca tgaccagcca ccacggagag gtcatatttg
    29221 ctttggacga actccagcag gccccccgat tcggcctcga tcagctcctt cttcagcttg
    29281 gtcaggaacg ggatcgtcag aggctggcgg ttttcgcagg cattcgcgat gaggttctgc
    29341 aactgcacga gcttcaaacc cgcgcccagc ttggcgagca gttcggcggt cacgtcggaa
    29401 cccggcggca ggggtgtcgc ctccatctgc gcttgaacga agtccgtccg ctcctgctcg
    29461 ttgggcagag ggatttcgat agccgccacc cccggattct gcaccagccc ggcattgagc
    29521 cccgccagac tctcggtcac caggcacagc gtcacgtcag ctcgcagaaa actctggttc
    29581 tgcgcccatc gcttcagaat caccaggcag ttgcggtcct cggaccccat cgaggaggcg
    29641 tcgcctgccg gcgcgatggt atccgcgtac ccgatcgaga gcgcgatctt cttcctgtcc
    29701 agaacgcgca gccgcaggta gctctcgatc aaagcgagaa caccgtcggg attgcgaggt
    29761 aatccctggg catactttgt gccgtggtat gcgtcgtaac ccgcaagggc gcgctggaaa
    29821 tcctgctgca cttcagaatt ggcgaatccg aggcctccgc cacggtcata ggaaagcacc
    29881 aggtcacgcc ggccgaacaa cccctcgtcg agaaacttcg ccagcggagc gtactcggtg
    29941 tggccgccac gcttccaagc cacgaggtcg tgcacgttgc catgcaacac gaacatggcg
    30001 atggtacggg agtaatactt ctccgaaagc cggcgcgccc actcgggcag ggcgtcgagt
    30061 gaagctgtgt tgcctgtggg attcatggtc agtttcgtac gcggtcccgc ggcggcgggc
    30121 tctgttcggc catcggcagg tgcggctcgt cggcgtccgg cgcagccagc gcaaagattt
    30181 cctccatctc cttcacgctc tgctcggtct gctccacatc ctgcaccagg tggtcgagct
    30241 gtgcgctcac ttcctgcggg tcacgcatgg tcacggactg gtcgcggatc aactgcagca
    30301 cgtcttcgat ggcggagcat tgcgcttcga tcacctggga attttcccgg atcttctcga
    30361 acttcgccac acgcttcctg agaatctcga tgcgccgctg gttgatctcc tgcaccttcg
    30421 ccggcgcttt ggccagcgat tgttccagcg tcgcggcttc gttgcgcacc tggtccggat
    30481 tcagcgattt caggtactcg cggtgcgtgt gagcagcgta aaggagccgt acgtagccct
    30541 ccagcagccc cgccagctta gcctccatct gggtagcgaa aatctgcgac gcgtaggaga
    30601 gctgcgagta gttggcgcga atggcctcgg cgacctggag aacgttggtg taccggctgc
    30661 gcatctccgc cgggatgttg cggtacatct cgttgaggcg tttctgatgt tcccgcttct
    30721 gatcggcgta cttccaggat cggacaagac ggcgaaaagg gttgctctgc ggcaccagcg
    30781 ccacgtacat catttcgagt ccggccgcca ggaccaacgg cagcccagta cccgagatca
    30841 gcgcgaacag cgccgcgccg gccaatccga tccagttgta ctgccacttg agggcagcct
    30901 tgacgtagtc gtgctgcgag aggcccgcct cgtcgagctt tcgattgacg acgcccttca
    30961 cctcggcacc gaaactgggc ttgtcatcga gcatcagacg ctggcctgcc ttccgattgt
    31021 ataaggcggc tgcggcatgc cgccacgccc gccagatcaa aggctgaccc ggacttgccc
    31081 tgagccggcc gtccggcgca ggcgggcaag ggcgtgggat tccagttcct ggaattcccg
    31141 ctgttcctta agcccttgct cgcgcggacg cacgaacggc accgggattt cttcgacaat
    31201 ggtccccggc cgcgcactga caatgtacag acgatcagcc agaaagaccg cctccgcgac
    31261 gtcgtgtgtt acgaacagca ccgttgccgc ggtctcgtcc cagatgcgca gcaggagggt
    31321 ctgcatgtcg ttgcgggtct gcgcgtcgag cgcgccgaag ggttcgtcca tcaacagcac
    31381 ctgcggtcgt accgccagcg agcgcgcgat cgccagccgc tgctgcatgc cgccagagag
    31441 cgtgtgcggg tacgcgttct cgaaccccga gagccccacg gcctcgatca ggtgctccac
    31501 gatctggcgc cggtcagtct cctgcgttcc cttgatgcgc agcccgtact ccacgttgcg
    31561 tgccacgctg agccaggcga atgaagtgta tttctggaag accattccgc gatccggccc
    31621 cggccccacc acccgacggc cgttcaccat tacttccccg gcggtcggaa agtccagccc
    31681 cgcgatcata cgaaggatcg tggacttgcc gcagccggag gggccgagca gcacacggaa
    31741 ttccccgata tcgcgcccgt ccgccgcaaa ctcgtcggcg atttcgaggt tgacgtcccg
    31801 caaggcttcc accttcttac ccgaggcgcc cgtgaagacg cgcgaaacgt tccgcagcgt
    31861 gattctattt tttggttcca tgggcggagg aaatggccgg ctcggattgc tcccgcgccg
    31921 ggcgctgcca agggaagaga cggtcttcca ataccgtcag cagccaccgg aacaggaatg
    31981 cggccactcc gatggcgatg atacctgcgt agacgcgctc gaaatccagc accttgcgcg
    32041 cggcctctac catgtagccg acgcccttgc gcgcgtttac catctcagcc agtatcacgt
    32101 aggtccagcc gatatcgtag agaatccgga atgagccaaa aatagcggga aacgaggccc
    32161 gaaacaacag ccatagcacg tggtggacct tcgcgcccag agtatacgcg gtctcgagca
    32221 gcgcgtggtc cacacggctg acctgttcca ccaccagcgc cagaaggtag aagaacatgc
    32281 cgaagaacag gaaccccacc ttcatgcctt cgtccatgcc gaacagcgcg atgaacgccg
    32341 gaagaaacgc tgtgatcggc atcgaccgca tcggcgcggc tatcggattc acaaggtgga
    32401 acacccacgg aaagctgccc atcaacaacc ccagcgggat cgcgaccacg gcggaccagc
    32461 agaaggcctg tgcaatgcgc caccaactct ggaagacatt gccgagcagg tcgtactcga
    32521 cccacagccg agcgatcgcc ttgaccacgc ccaccggctc tggcaacgcc aacggtggca
    32581 gcaggttgct cctggtcacc aggaaccaga cagcgacgag gactgtccag gcggcaaacc
    32641 cgagaactag tgtcaacggc cgcgcgatcg gtttccggat gcttgtgggt tgcaccatca
    32701 ctggttcgcc ggattcggat acaccttgat gtccgtacgc cggttcttct cacgtccctc
    32761 gggcgtttcg ttggtttcaa tcggtttgtc cggcccgttt ccagccgtcc gcatgcgtga
    32821 tgccgggtag ccgtacttgg tcatcaagta gttctttacc gtgtcggcgc gttgtctcga
    32881 gagagtcaag ttcaactcgc gcgagccggt cgaatccgtg ttgccatcga tatcaaccac
    32941 cgtattctcg tatgcccgca tgaagccacc gatctcctcc actaccgccc gcgaatccgg
    33001 gctcagagcg gccgagttgg tctcgaagta gatggaccga cgctgcgtgg cgatgggtgt
    33061 cgcgcccttg ggcggagcct tgtactcgat gggtgcctcg gtcgactgcg aagagaactt
    33121 gccctccatc gcagcgatgt agcgccggtc cacgctgccc tcaatatcgg gagcaccctt
    33181 gatcaaccgc agttcgcggt acatgtcctg cgccatcccc atgatattgt cgtagtcgga
    33241 accggcgccc gagccgaaga aggctttgtt gtccgcgtag tcggacagcc gcacgcctcc
    33301 cagcatggtc ttggcgaggt cttccggaat gttgaagtcc ttgatggtgc cgatcagcgt
    33361 gtgtgcccgc gtgggctgct gccgaatgaa ctcgacaccc tccagccacc cttccaggaa
    33421 attccgcagt gtctgcggtg agttggccga gaagcggtcg ctgaccacca ggatgtcggc
    33481 gatgagccgg ttggcgaccc gcgtgtcgta gatcttcttc gcgccgggcc gtttgcccac
    33541 cgcgtcggtc atgtccggat cccaggccac cgcggcgtcc actttctgtt gcgcgaacag
    33601 ggtcgctggc tcgattccgt ccttggtatg gactgcctgg gtgaagatct cgttgcgctg
    33661 ctccgtgttc aggcccgagc ttttgagtcc gttccatagc aagaaatggg agggcgtgta
    33721 gggcgcgaag gcgacgctct tccccgccag gtcttcgatg ctgttgatgc cctgcttgcc
    33781 gatgacaccg tcggcgccgc gcgaccaatc caccatatac acggcgcgcg cccggaagcc
    33841 cttatcgaga agctggccat agtctttggc ccacacgtcc gcggttgtaa gcatcacgtc
    33901 cacgttgtta gaggccagcg cggccgtgcc ctcggtccag tcgtcgatga tcttgaaaga
    33961 aaccttcagg cccttcttgt cgtagatcga accggccgcg gtgtccagcc cgccgttggc
    34021 cacaaggcct cccacgcagc cgacccaaat gttgacgcgc acgcgcacct ggttcgtttc
    34081 cctgtcgccc gtggtggcga ggggcttcga agtatccacc gtcgtggtgg ccgccttctt
    34141 cccgaataat ccagacaggt tgacgacgcc gtaccggtcc aggccgtatg cgaccagccc
    34201 gagaccaatc agaaatatga agatccatcc gcctttttca attcgtaccg ccatgacttc
    34261 ctccgcaaac tacgcagtct tctcggtctc ccggccgccg atagtcttct cgggcgccgg
    34321 ggtggatgct tcgggcgaaa tcaggcccat ttcggccttg aactgtttga ccagttcgct
    34381 agcgcgcagt ttctcggcct cttcttcgat ttggaacttc tgctggtcca cgttgcccaa
    34441 ggccatttcc atgcgggcct cgttgactgc cgtggtttct tcgatcttgc ggaccatctc
    34501 gtcgtgggtc tgactgaccc ccgccacttc gaactgctcc atggcgtcgg ccactttctt
    34561 ctgccactgc gagcggcggt agtcgcgaat cgcgttcatg gcttcccgcg tcttccgctc
    34621 tttctcacgc aggaaggcgg ccttgacgtt ctgtgccttc tcgaagcccg cccgcgcggt
    34681 tgccaactgc ccctgcgttc gtgccaatgc gctccgcacc tgttcgagct gcacggcaaa
    34741 gtgcccggcc agatcgtcgc ggcccgcctg tatggcagcc tttaccttgg tcgccagatc
    34801 gtttacatcg gccttatact tgttctcttc tttttcgagc agcgtggcgt tggcctttac
    34861 catcgcgatg ctctcgttca tgcgcggcac ctggtcgttc atgtcgcgaa tgttctgctc
    34921 gaggatcagc tcgggatcct ccgccacaga aataaagaat ccaaagaacg aacgcaaaat
    34981 acgcgaaagc cgcttaaaca tgagatcgtt cctccttccc ggctagctgc cgggagtctc
    35041 ctcgggcttg gccggagcag cggccggccc ggttgtaatc gggttctccg ttgcgaatgg
    35101 cgccactgcg tcagcgatgc ggcgctcttc ttgctgcttc tggagccgcc aggtctgcat
    35161 gcgttcgcgt tcgcgcgcga cggcgtcgtt attcgcctgg atgctcgcgc gtaattccgt
    35221 gagcacgcgg tccgcttcct gctggagctt cttgttctcg tccgacttct tggcttccag
    35281 ttgatcgagt gcgcgctgct gcaccagttc gaatgcatcg agcgcgcggt cgcggcggac
    35341 cgcgtcttca atgatttctt gcagcttcac gccggccgca tcgagcgcca gcaacacgga
    35401 actgcgtttg atctccacgg gcaggctgcg aatgtgctcg ctcttcaaca tgtccgcgat
    35461 cttgaaaatc gtgaagccgt gtgccggggt gcgcacctcg gcggcctggt agatctgctc
    35521 gaacgcggta gggtccgcga ccggctgggt aaacgtgggc gtggcctttg cggcagcggc
    35581 aatgtcggcg atggtctgcg ccgcggttcg tgcgcttccg ggcggagtat ccggggctgg
    35641 ttccccgccg gccccagcgc ccggattcac tggatcaacc gagacgaagt agttgtacca
    35701 cttcttactc atcgggcatc tccctcattg ttccttgggt aagggtaaga ctatcaacgg
    35761 gttgaagtca atcgggcgct gtgctctatg gaaggctcac atttcgccat acgaagccag
    35821 cgagcgggat gttcccgaaa acgaatttcg gaccaccgcc cgatttccga aaggctacgg
    35881 ataaggcagc ctgcgtcgtt aatgttaacg cagcaaagta gaacaactgc gggatacgcc
    35941 ggcaggcctg ccgccgatct ggccagggcc gaggacacac tcttgatcga aaagccgtaa
    36001 gatggtgggc gattgcgcgg aggttcacca gcaggtggaa cgcttcgacg gaaaggcgcc
    36061 ccgcccgtat gccagtacag aagaggacgc gagcgggtat cttggatgga ggcgatgcgg
    36121 atgaagacgc actcattgtg cgcaatggag ttttcgtgtc tgcagggtag gaaccatgac
    36181 aaaatctgaa cagttctcgt ggaggcggcg tgatttcctg ggtgcgacgc tattaccggt
    36241 ggtctgtcat gccgtcgcgg caccggacga tatggtcgcg cgttccgtca agaacctcaa
    36301 actgagcgtt agccggaatg ggcgcttctt cgtggatcag aacggcaagc cgttcttcta
    36361 cctcggcgac acttgctggt tgctgtttca acggctgaac cacgcggaac tggacgagta
    36421 cctcaaggac cgcgctggca agggatttac cgtgatccag gcctatgtca ttcgcggact
    36481 ggataagaag catccggatg ggaacagctc gcttctcgga gagtcaccgt ttatcgaccg
    36541 cgaccccacc aaacttaacc ccgcattttt cgcgaacgtg gactacgtca ttaaccgagc
    36601 caacgagctg gggttggtga tgggcctggt cacctccaag tcgtggcacg tcaatgaaca
    36661 cccggagcgt gtcttcgatg cgacgaatgc gttcaccttc ggaaaacttc tcggccagcg
    36721 ctacaggaac aacgctgtcc tgtggtatgt gggcggggat tcgccgccgg gcaaagacga
    36781 ggccacatgg gttgcgatgg cccggggact gaaggaagga aatggcggaa gtcagcttgt
    36841 ctgctatcac gggcagggca gcacttcttc atccatgtgg tttcacaatg ccgactggct
    36901 cgacttcaac tcgatccaat ccgggcatca tttcgggtcg gacagctacg cgtttgtcag
    36961 caaggactac gcgttgacgc ccgccaagcc gaccgtggac atggagccgg cctatgagaa
    37021 ccatcccacg ggcgccaaca agccgcgcgt tgacgcccac aaggtgcgtt ctcaagccta
    37081 ttcggccatg ctcgctggcg cggcggggca cggctacgga acgttggacc tgttctacct
    37141 ttacaaggac gccgacggtc cgttcccgaa aaacggcttc caacactggc gcaccgcgat
    37201 ggcctacgag ggctctcgcc aagtggggct gatgcgccgc ctgttcgagc agcgaccgtg
    37261 gcacaagatg gtgcccgacc aatcagctct cgcctcggcg ccgggcgacg gtccgtaccg
    37321 cttggttgcg gcgcgggccg aagacgggag cttcgtgatc gcatacacgc cgcgcggcgg
    37381 gagcgtgagc attcacatga ataagttcaa cggctcgcca gtgaaagctc agtggtacga
    37441 cccacgcaac ggaacatgga aagcgatcgg ccagtatgcc agccaaggca tccaggagtt
    37501 tgttgcgcca tcccacggcg agcaagatga ctgggttctg gtgctcgatg tcgtgccgtg
    37561 aaagcgagaa gcggtgggtc aatttggttg tgctcaccaa acggcggggc agcttcgaac
    37621 acgatgacca tcgccgtgca gtgcgcgtga aagcggaatg cgcgccgtag cgatgccgac
    37681 tgccgaacgc agattgcatt tgcagtgtgg gaggaatccc aggaggaggt cggcgctcgc
    37741 aaaatgcgca cgggatgggt tcctggccct tcgcgtccga cctgtggccc tcgcgcgcgg
    37801 ggaacggagt gtgtggtttt ctgcgattcc gtcggcgtga tcacctccgg aaaccgactc
    37861 caataaactc agggagcgac gatcgacacg gtcctggacc ggtccctgat tcttgaggac
    37921 gaaaggcgtg ccggcccttg cctggcgtcg gcggaactgt gtgtgcgcct cgaccgtaca
    37981 atcgggtatg gacggcgtgc gggtcttact agaagacgcg atcgcccagt ggcgggcgtg
    38041 tgatgcgtct tttccacaag ccacaccccg gttcagcgag cacgagcagg ctccccgcga
    38101 ggagcaactg gcgcggtttc tgggcagcct ggaagccgag gtagcgcggc tgcctcgcac
    38161 caggagtgac cgcgacggcg tgcgcgagcg cctcaccggc gccttcgtcc gcctcgccaa
    38221 gtccgggctc gggctggagg accggcacct cgatctgctg ctcggcggcg gcttctcagg
    38281 agtcgggacc gcactggccc gccgggcgcg gcggttcgac cccgcagtga gcacggccga
    38341 catcctccag gcctgccgca acgcttggac cgcctgcggg ttgcaggtgc tcctcggtcg
    38401 tccgatggag gtgacgcctt cgatcttcgc ctacagcatg ttgtaccctt acagcgacaa
    38461 ctacctggat gacccgaagg tcactcgcga ggagaaggtc agctttagca gtcgtttcgg
    38521 ccggcgcctg gaaggagact ccgtcagtcc ggcgaacgcc catgaagaaa acatctggcg
    38581 actggtcggc ctgatcgagg gccagtatct ccggtcctta tatccgcagg tctactcgag
    38641 cctgctcgcg attcacctgg ctcagttcga cagcctgcgc ctgtctcgct cgggcgccgc
    38701 tgccggcgag gtggacgtct tgcggctcgg cttcgccaag gggggaacgt cggtgctcgc
    38761 ggacggttac ctgtccgccg gttcgctgac ggccgaggag gcgcgcttca tcttcctatg
    38821 gggcgtagta ctgcagctcg gagacgatct gcaagacgtc cgagatgacc aggcgggcgg
    38881 tattcttact ctcttctccc aagccgcggg aaagaagccc ctcgacgcgg tcacgagccg
    38941 caccttccat ttcggcgagc gcgtcatgag cctgctggag ggcgtgggcg tggcggagtc
    39001 cgggacactg aaggaactga tgcggcgtag ctcgcggtcc ttcctgctcc gttgtgcggg
    39061 cgaggcaggc gagttctaca cgccggaata cctggcccgg ctcgaagcgt gctcaccgta
    39121 ccggttctcg tttcttaacc gccgccgcga acagttcacc cgccgccagg cggccttcac
    39181 gcgcctcttc gaagccttcc tcgccgccgg cgacgacgag cccgccttcc ctcttctccc
    39241 cagcggcctg atgccgagac tgtgagccag ccgaccggac ttcggcgcct ggcttgtcgg
    39301 ccgccttctg tcggaactgg agctgaccgg cgcttcggta cgtgatcgcg ggtctggaat
    39361 tcacacttct tccggtgcat gtcaccctgc ttcgacgacc acggcttcga taccgacgtg
    39421 gcaagcccga ccttcgggga gtggaacgga gcggtgacac ccccgcggac gatacaattc
    39481 agcgggaagt ttgtattctg aggaaatcgg gttgaaccat ccgcgggttt cgttgccgta
    39541 gcctcgtgcg gcggcttgcc tgtcgatagg agggggtgtc tatgatgctg ggcccaggtc
    39601 gcatggcgtg ttcccactgg ctgggcggat ggttaccggt ctggacgaat cgtagcagag
    39661 cggtcctctt cgcactcgtg tgctcgccac tgatgtcggc cgcgacctgc tacgcgcagg
    39721 aatggcggtt ctggggaggt gacgcgggcg ggacgaaata ctcaccgctt gaacagatca
    39781 acaagaaaaa cgtgtctgtg ctgaaggtcg cctggactta cgacacgggt gactggagcg
    39841 acggcagcga atatccaacg cgctcggcct tcgaggtgac gcccctcgtg gtggacggcg
    39901 tgatgtacgt gagcacgccg ttctgccgcc ttatcgcgct cgaccccgaa accggcgaga
    39961 agcgctggga gttcgatccc aaaattgacc gtgcgatgcg caccaacctc ttcgtcagcc
    40021 gcggcgtgac gttctggcag gacggaaaca agaagcgcat ttttctcggc gatctccatg
    40081 gctgcctttt ctcgatcgac gccatgaccg ggaagccgga cccgaagttc ggaaagaatg
    40141 gcatgctcga cctgagagct ggtttggctg accaattccc cgacaaacag tacaaactga
    40201 cgtctcctgc cgcagtttgc gggaaggttg tcatcacggg aagtctggtg agcgatggca
    40261 cgccgaaagg accaagcgga gacatccgcg ggttcgatgc gcgcaccgga gagcttctgt
    40321 ggcgcttcca taccgtgccg cgcccgggcg aattcggcaa cgacacctgg cagggcgacg
    40381 cgtggaagga ccgcggaggc accaatgcct ggtcggtcat gagcgtggac gagaagaacg
    40441 gcctcatctt cgtgcccctt acttctccca gctacgacat gtacggcggc gaccgcaagg
    40501 gcgcgaatct gttcggcaac tctgtcgtcg ccctcgattg ccgaaccggg aagcgcaggt
    40561 ggcatttcca gacggtgcac cacgatgtat gggactacga cctgccggcc cagccggtcc
    40621 tggtgactgt tcagcggcag ggcaaggacg ttcccgccgt ggcgcaggtc acgaagatgg
    40681 ggttcacgtt cgtgctccac cgcctcaccg gagcgccgct gttcccggtc gaggaacggc
    40741 ccatgccgaa gagcacgatc cccggcgagg agacctggcc gacgcagcct gttccggtga
    40801 agccgccgcc gttcgcgcgc cagtcggtta cgcgcgacga catcaccgac gtgacccccg
    40861 aatcgcgcaa ggaatgcctc gaaatccttg gcgacgcgga cgtggacgtc aaaatctatg
    40921 atccgttcgg cgagcaggat gcgatcatca tgccggggct gaacggaggt acgaactggg
    40981 gcggggcttc cttcgacccg acgaccaggc tgctttacgt caattccatg gatgtcgggg
    41041 gattgttccg gctggtgaag cgcccagaag ggtccgagat tccgtacgcg ctccgcgcga
    41101 ggaaatacga attcctctgg gattcgaagg gctatccttg ccagaagccg ccgtgggggc
    41161 ggctgaccgc gattgacctc aacaccggcg acttccgctg gcaggtgccc ttgggggagt
    41221 tcgacgaact caaggcccgg ggtgttccca aaacggggac gccgaacatc ggtggctcgg
    41281 tggtgacagc tggcggcctg gtcttcatcg gcgccacgaa cgacgggaaa ttccgtgcct
    41341 ttgacaaaga caccggtgcg gagctttgga tcacctcgct tccggccagc ggcatggcca
    41401 cgccgacgac cttcttgggc aagaagaccg gcaagcaatt cgtagtcatc gcagcgggcg
    41461 gtggcaacaa gtacgacaag gcgttcaccg gcaagctggt ggcctttgcg ttaccgtagt
    41521 actctgtcga tgcaaagcgt tgcatttgcc atcctcgctg cactcctgct cgtgagcctg
    41581 ctcgctcagc gggctcctga gtataaggga aaggacgaga aactcccgca ggccgtcgct
    41641 ccacagccgg tcgcttacag ccacaagaag cacgtcgcgt tgggcatgcg atgcctcgac
    41701 tgccacaagg gcgcggctca agaagatcag gcgggcctgc cgggcacggc tctgtgcatg
    41761 gactgtcatg cgacgatcaa ggccgagagc ccggaaatca gcaagctcgc cgaaatccac
    41821 aaacgcggcg aaaagctcgg ctgggtgcgc gtgtatagag tccctggttt cgtcttcttc
    41881 agccacgcca gccacgtgaa ggccggcgag cagtgcatca cctgccatgg ccaggtccag
    41941 gcgcgcgatg tcctcgccaa ggaagtgtcg acaggcatga tcgcttgcat gaactgccac
    42001 gcagctcgga aggtctccaa cgactgcgtg ctctgtcacc agctcagttt ttgacgggaa
    42061 caaaagcgcc cggggactcg ccccgggtcc agggcggaat atcgccgcga cgcagggagc
    42121 cgagtcgctt gctgagccta tcttcaggcg tccagatccc cctcccaggc cacttctgcc
    42181 gccaggcatc gctcaacggt gtcgcgcact gggctcgtcg tcgccttcgg atcgtcggac
    42241 accacagtga tggcggatcg aggaagactc ctgcgtccgc cgcgtggcga gccgaaccag
    42301 aactgtccag ctcacagctc ctcagctaga tgataactct cgaaaacgca cgagcattcc
    42361 actcacggat tcccggcagt gatcttgaag cggacctgct gctcggcggg tttgacgtaa
    42421 ccgcccggca cgaacttggt ccggagtgga atcgtgagtc ttcccaccgc ctgatcgagg
    42481 ctcgtctccg gtgcatcggc gtacgggttg tgcgggaaca cgggccacga aagccttgcc
    42541 gccggatcga cgctcatcgt ccagccgccg tgccggatca aaccgcccag ttccgcagcg
    42601 gataactcga accgttccgc gcccaacgtg atcttcctgc cggcgcccgt ctcgagtacc
    42661 gcaccggact tcaggaccag ttgcaggttg agcagggtct cgtcagaagg ccggcccctt
    42721 ccgttgacca cgacctggaa ggacatttcc ttgtccgatg gcggccccat tagcaggtcg
    42781 acccagaaag tgttgtatgc gagcgataga cgatcctgcg catcgctcat ctggaggcgg
    42841 ctgctcagcg gcatgtgcac gatctggccc atcagtttct ctgagaacgt cgccagctcc
    42901 ggctgccgct tcgagtttcc gccgttgatg atcatcccga gtttctcgtg aaagacgcca
    42961 agattcccct gacgatcaag gtagaagcgg ctgttgatcg cctgcgtgtc tacgatcccc
    43021 gaaaggacca ccacccaggg acccgtcttg cgaattcccg ctggaatttc catctggtgg
    43081 aagtatcggg actgctcctg aggcgccggc gatttcggtc cgtcgtggta atagagcgca
    43141 tcctgcgcca gacgcccgag ctggtcgatg gtcagcgttt cgggcttgaa gaagtttgcc
    43201 agaaactccg cgtatccgcg cccgtcggcg aagttcgaaa aagcaaagtg cccccacgcg
    43261 cttacggtcc ggttgcggtt gcggtcgtcg atcacctcga cgggagagcc gtcgggattg
    43321 gtgaagtact tgtgaaagtc cgtgacgcgg cgcaacgctt tcagggctgc ggggtccttg
    43381 ctcagttccc agtacagggc cacgctggtc agggtcaggt ggttgtaacc tgtcgttggg
    43441 ccgcgccggc tgtgttcgcc ccagtagcca tcgggcgtct gctcgatcgt cgcgaagcgc
    43501 tgcaggattc gcgctccgag ttcctcccat tccttgttgc gaaacacctg cccggccagc
    43561 agcagattgc tcgcccactg ggcgtaatga ttcggcgacg tcccgatgta cggagagttg
    43621 taccacggga aatccagcct cgctttggcg tcatccacca gcagggcgag gtttcgttcg
    43681 atggcgcgtt tccacctcgc gcggcgctcc tctccgagtt ccttctcaag caatcggtag
    43741 gcttccaacc acatgtaggt gtcccagtcg ctgtcaccgc gcggctcaaa ggtccctttc
    43801 tcgtcctcac tcgcgagaag gtcgccaatg cgaagcgcca aagctagcat cttcgggtcg
    43861 tgatagcgcg ggttgtcccg gtgctgcttt gcatagagaa ccgtcggagc aagaatggcg
    43921 tacggaaaat ggcgagatcc agactgggat tcaagcgttt tcaaatccgc gtcaggctgc
    43981 gcgttcaacc gggactccac cttgtccgct ccagcctcca agatccggta atagcctccg
    44041 ggcagggaca ccgcctgaag cgaaacggcc gcaaggccga ctgacagaac tcggcaaagc
    44101 gaatgcatct cgtcgctcct ccctcatgga ttgtagccag gattttcatt tccgctgatt
    44161 tggcttcgac cgccagtcca acgggatggc ggggggtata tactgtcgcg aagccgggtg
    44221 ccgagaagga agtgtgaatg cgcttgatag accgtgaccg tcggagtttc ctgaaaagct
    44281 gcgttgcggc accccttgct gccgtcgaag taccccaggc caagccaggc tacaggacct
    44341 gggatctgca cgttcatatg ttcggattcg ccggcgccac gcccgaggag ctgatcgagt
    44401 cgctggtccg ttttgcggac cggatggagg ttgaacgctg ggtcctgtca aacccgctcc
    44461 acgcggatcc gactccggag caattccgtg atgccaacga tgccgtgttg cgcgccatca
    44521 aacgtgcgcc cgaccgcacg cttggcttgg tctacctcaa cccgaatcac ctccaggcca
    44581 gcctcgacga actggaccgc tgtgtgcgtg acggacccat gatcggggtg aaactctgga
    44641 tcgcgagaca ctgcaacgcg ccggaactgg accccatcgt cgaacgagcc gctcagtacc
    44701 agtcgatcat cttccagcac acgtacctga agaatcaggg caacctgccc ggtgaatcga
    44761 ccccttccga actggcggaa ctggccaggc ggcacaccag ggcgaagatc attcttgggc
    44821 acaccggggc ggattgggaa cgcggcgtcc gctccgttcg cgacctgaag cacgtgacgg
    44881 tcgatctggc cggctccgat ccaaccgcgg gcttcacaga aatggcggtg cgcgaattgg
    44941 gcgcggagcg ggtcctgtac gggagcgatg tgggtgggcg cagtttcgct tcgcaactcg
    45001 ccaaggtatt gggcgcgaac atccccgagc aagccaagat agatatcctg ggcggcaacc
    45061 tgcgccttct gctcgcgccg atcatgcggg ccaaggggat gcgtgtatga tcatcgatac
    45121 caacgtccat ctctcgcgct ggccgttccg gcggctggcc gacgacgagc cgggggcact
    45181 tgtcacgcgg ttgcggcggc atggtgtcat tgaggcctgg acgggaagtt tcgatggcat
    45241 cttccacaag gacatcggcg gggtcaatgc acggctggag gaggattgtg cgcggtacgg
    45301 ccgagggctg ctgattcctt tcggaacggt gaaccccacg ctgcccgact ggagagaaga
    45361 cttgcgacgt tgccgcgaag tccaccacat gccaggcata cgcgtgtatc cgaactatca
    45421 cggctatacc ctcgacgatc cgaagttcac cgagctactg cgggcggcga ccgatagcgg
    45481 agttattgtt cagcttgtcg tacagatgga agatgaccgc atgcagcacc cgctgctgaa
    45541 aatccccgcg gtggacctgg ctccgctccc gacgctgact cagacacatc cgaaactgcg
    45601 tctcatgctg ctgaacggat tccggtcgct gaatggggga gcagtgaaaa cgctggtgaa
    45661 tgcgccgggt gtccgctttg aattcgcgat gctggacggc atcggcggtg tgggcgatgc
    45721 agtagaagcg gtctcagcaa accgcctcct cttcggttcg catgccccgt tctactattt
    45781 cgaatcggcg cttctcaagc tgaaggaggc aggactacca aaggaagtcg tggacagaat
    45841 ctgccaggca aacgcgcggg aattgctgcc ccgttaacgg tcaacggcac tgaaaggaga
    45901 gccatggacg accagaatgc ggcgggagcc agggttgctg cgacggctga ccatccggcc
    45961 gagcctgtcg tcgcccccgt gaaggagcgg ctgcaatctc tcgacgtgtt ccgcgggttc
    46021 actctgctgg ggatggtgct ggtcaacagc cacccgggca agatctttcc cggtctgggc
    46081 cacgcgagtt ggaacggatg gggctttgcg gacttgatct tccccttctt cgtgtttatc
    46141 gtcggcgtgg ccatgccgta ttcgttcgcc agccgcttgg cgcgtggcga cagccactcg
    46201 aaactcttct ggcaggcgct gcgccgctcc gtgttgctgt tcgtggtggg cgtcttcctc
    46261 aacggttatc ctcagtttga tctggcgacg ctgcgcgtca tgaatgtgtt acagcgcatc
    46321 gccatatgtt atttcctcgc atcgattctg tatgtccacc tgcgcctgac ggccaggtcg
    46381 atggtgtggc tctgcgggac catcctggtg ctgtacttcg tgttgatgaa gttcgttccg
    46441 gtgcccggtt tcggcgctgg cgtgctcgag cccgtgggta actgggggaa ctacatcgac
    46501 caactcgtca tgcccgggcg tttgggacac aagacctggg aaagcaagag cctgctcggc
    46561 tgtgttccgg ccctggtgac gatgctgatg gggttgctga ccggcatcta tctgcggact
    46621 cgacggccag cgtacgaaaa gcttgcgaac ctctacttct atggctccct gtgcatggcc
    46681 gcgggggtta tctggacccc ctggtttccg atcaaccagc atctctggac cagttcgctg
    46741 gttctgttca tgggcggggt cgcgttggtg ggtctagcca cttgttacta catagtggat
    46801 gtcagaaagg tcacctggtg gaccctgcct tttctgattt tcggtgtgaa ctcgattgct
    46861 gtctgggtct tctcgcaact tggcatgaag accctcatgg caatccaaac ggtcgccccg
    46921 gatggtacgg caacgaatct gtggaaagcc ggcggcgata ttctctcccg tcaccttggg
    46981 gaaatgggcg gaccgttcgt tttcgctgtt ctgtttgacc ttttctggct gggcgtgatg
    47041 ggcattctgt actggcggcg gatcttcatc aagttatgaa acggaggaat ccgcctcggc
    47101 tcgctgatgt ggaatcagca gcgggcagca aatcgcgcgt gatagaggaa tgtaatctcc
    47161 aggttgagta tctgaaggga gcgtcgatga gccaagatca aaagtcatca cggagagaac
    47221 tgatcaagca aagcgttctg ttaggagcgg gaggggctgc ggcccccatg tttattccac
    47281 ggcgggtgct cggagcgcct ggacggccgg gagcgaacga ccgcatccgg gtcggcatca
    47341 tcggcgtcgg gaaccgcggc agtttgctga tcgatcagct acccgagggc gccgagatgg
    47401 tcgcggccgc tgactgctat ctcaagaagg cctacgatgg ggcctcgaag aggcaggcca
    47461 agtggcacat ctaccaggat taccggaagc tgctggagca aaaagatatc gacggtgtga
    47521 ttgtggcaac cggcgagcgc tggcacgtgt tgtgctcgat ccacgcgtgc caggcgggca
    47581 aagatgtcta cgtagagaag ccgatgtccc tctatgtcga agaggggcgg gtgcttgtgc
    47641 gagccgcgcg caagctcaag cgaatcgttc aggtcggcag ccagcagcgg tccatggcga
    47701 tgaaccagct tgcatgtgac ttcgtgcgga gcggtggaat cgggcgagtg ttttttgtag
    47761 agggcaagaa ctacggcagc ccgcagcccc cgcagtcggt ggagtcgtca gctatgtcgg
    47821 agccggtgcc ggaaggattc gattacaaca tgtgggtcgg ccaagcgccg atgcggccct
    47881 acagctccaa gggtggtggc ggatgggggt gggacttcgg tggcggcgac atgacgaact
    47941 ggggcccgca cggactcgat caggtgcagt cggcgctggg aatggatggg tcggggccgg
    48001 tggagttctg gctccttgcc gaggggcccg gcccggaagg ctccatggcc ttccgctacg
    48061 caaatggcgt gaccgtgcgg ttggagatcc ccaccggcga cgtgcaggga ggagccatct
    48121 ttgtcggcga gaagggccgg attgacgtga ttcggaatat gttccggaca gacccgccac
    48181 agatggtcag gcaactgccg ccccccgaag aagtgcagaa gtggcgcgac gacgttgcgc
    48241 tgtggcaggc gaaatatcac atgcaaaact ggctggattg catgcggact cgcaagttgc
    48301 ctctggcaga cgtggagatc ggacaccgtt cggtgaccgt gtgccacttg gcgaccatca
    48361 cccgcaggct cggccggatg ctcaggtggg acccgcaagc cgagcgcttt atcggtgacg
    48421 aggatgcgaa cagcctgttg agccgccccc gcaggaaggg atacgagttg cccacggtat
    48481 aggcggtaag gcagccgggt atatactggc ccgcagcaag gaggcggaga aaaagagcgt
    48541 gaaaaggatc gggttctgct tgactgtttt tgtcgccgga gtgttgtttg gtcttggcgg
    48601 gcttcgcctg tgggatcact ccactgccgt ggcacagggc cttggcactg cctatcctcc
    48661 ccatccagcg gcgagcaggc caggcatcac tattgaccct acggccttcg tgcatcccag
    48721 cgtgatccta gagggaacga tcaacatcgg cccgtatgcc taccttgatg ccggttccat
    48781 cctgacgggc aacgtcaccg ttggccacca cagcctgatc cgctgcaatg tcaccatccg
    48841 cggaaaggtc cggatcggta actatacgca tatctacgac aatgtcaaca ttgagggcgg
    48901 ccgaccggcc aaaccactcg gcggctcgct ggccgaggtg gccgatgagg cgatcatcgg
    48961 ggaccactgc tggatcaacc atggtgcgat catgcacgga acgcggatcg cagatggtgg
    49021 agctgtcggt ctgggagcgg tctgcgacta caacacgcgc atcggtaagg acgccgtgct
    49081 ggccgacgga agcgcaacgc gggttgacat ggtgatctgc gacggctgtt tcttcgaagg
    49141 cgttcctgct gtcgtgaaac ggaagggcct aaccgagaag gaccgggcag agtatttcgg
    49201 agtctcgccc ctggcctgga caacttttga gggcgaccgg caagaagcaa acgccaagaa
    49261 gcgcatgggc cagaagtaat ccccgctgta cccttcgttt gtaaccgcaa gttgaagagg
    49321 cctctctggc aaacgcccgg gacgccaact caggacgaac caggaggtcg gatagtgtag
    49381 ctttccgtgc gaccttctga caaaatgcta ccgtcatgca cctttacaca tatgctgtgt
    49441 tgaccggaac gttcgtttgc ggtctcttgc tggaggccca agaaccgccg cgcccattga
    49501 tctcgcccga ggtacagcag gacaggcgcg tcaccttccg gctccgcgct cccaatgctg
    49561 agcaggtttt cctcgccctg gacggctcga aacgcgccgc gatgcagaaa ggcgaagacg
    49621 gagtctgggc tatcaccgcg ggcccattgg agccagacat ctacagctac tccttcgtcg
    49681 cagacggcgt cacgctgctg gatccgtcca actcatcgat aacgctcaat ctgctgaatc
    49741 cggccagctc ggtgcacatc cccggggagg gcttggcgtg ggatgtgacg aacgtgccgc
    49801 gcggcgtcgt gcaccaccac ttctatcgtt cgaatatcgt cggagacgac cgtgacttct
    49861 atgtgtacac cccgccgggc tacgatccac gcgcgaagaa gccgtaccca gtcctatacc
    49921 tgctgcacgg tttcagtgat gatgccggca cctggacctc cgtcgggcgt gcgcatgtca
    49981 tcctcgacaa cctgatcgcg caaggcaaag cgcggccgat gctgatcgtg atgacgctgg
    50041 gctatggagc tccggaaatc ctgtcgcgca caaggcccgc gttccgtgac cccgggctgc
    50101 ggcaacgcag cttcgaaaag ttccgggatt cgcttttcga ggaggtgatg ccgagagtgg
    50161 agaaggaata ccgaacctcg aacgaccgca agatgcgcgc catcgccggc ctctccatgg
    50221 gcggcgcaga atcgctgtac attggcctca acgctctcga ccgcttcgct ttcattggcg
    50281 cgtttagttc cggcggcctt ggcgacgatc ttgccgctca gttcccgaaa ctggatgaag
    50341 aggcgaactc cctgcttcgt gtgttctgga tatcgtgcgg gacggaggac aggcttctcg
    50401 acttcagccg taaattcgtg gagttcctga agacgaagaa ggtggaggtc acactgcgcg
    50461 aggtcccggg ttcgcacaac tggagggtct ggcgccggga cctcgcgaac ttcgcgtcga
    50521 tgctgttcca gtagactgag agcgcgtcga acaacgcgtt ccgctgacga atcccccgcg
    50581 gcgcgccgta gagcgagaag atcgggtgct cctcgctgac atccaattgg agtggtacgc
    50641 tgcagcttcc cgaggtagca tgttcggtgt tcagaagttg aattcgatga cccggttctg
    50701 gagtcgatca tgaaacgact ggttctttcc cttctcctag ctgttctggt tcttcctgct
    50761 gcggatctct atcacggcaa ggtgtacggc attttcgatc ctctgaaggt cagcgagacc
    50821 ccggtttcga tcaccgtttc gggcgggacg ttcaggtaca tcattgaccg cgccacggga
    50881 cagatcacgt ccgtcaaagc tctcgaagac gaattcgtgg ccgccggtgg agcctttcca
    50941 aatccgtatg tggggctgat gcccgaagac gatcccggtg cgcgccgcga gggaggcaag
    51001 gagcgcccgc gttacgggta cgagaagtct gtcgagatgc gcccgctgtt gtggtcgggc
    51061 gagctcacgg atgcgcaccg cttcgacgcg gcgaagggaa aggggattcg gactgagctg
    51121 gtgcgctctg ccgcggactc cgtcgaggtg cgctccagcg gccgatatgg ggacactccc
    51181 ctctcgtgga acatcaacta tctcgtcgac gtggacggtt tcactaaagt tactgtcaca
    51241 ctgagcaccg acaaggcggt gcaactgcgc tggcattgct tcaaccatgc cgtcctcgcg
    51301 aagaacgtca tccagtacct gacacgggtc agcgacccgg agcggccgcc gattgaggtg
    51361 cggcccttac cgaccgtatc gatcggcgac gtgcagccgg acagtcccat cctcgagtcc
    51421 cattggaacg ccttcttcca tctggcgaac cgggtaaccg gcatcgagtt ctccaagcag
    51481 gacttcgaag accgctacag cggttacagg gattctgcag tcattctcga aagtgggact
    51541 tcggtggaca ctggcgccgt cgggaccaag gacggccgga cgctccgggg ctgggattcc
    51601 cgcgggaaga ccaatatctt cactcagatc taccagcgag agcagggcct tgaggtggaa
    51661 gagttcgaca tccgcaacgc gacgtttccg ttgaatcccg gcgacgtacg cacacgcgtg
    51721 ttctgggtgc aacccacccc ggctaagcgt ccgcgcaacg atctgaacag cctgcgggtg
    51781 gtgtggccgg gacctcatca gatcgtcatg acacgctggc gtggcaggca gcagccgtgg
    51841 tcaccgccgg acgacgagca ggtcaagttg tgggcgcaga tgggcgtgaa cttcatcgtc
    51901 ggcggggctg actactggtc gggcgactat gcacgtccac tcgcgccgga aaagatccgc
    51961 catttcattg agacggctca ccggtacggc atcaaagtca ttccttacgt gaccttcacc
    52021 gacttcaatt tcacagcgcc cggctaccag gaacatgcag ctgagtggat ggcttcaaag
    52081 agcatcgaat acgccaacga gacgacgctc atgtgctata acgccgaggg ctggaggaac
    52141 catctcgag
//
LOCUS       DATIFQ010000121         2975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_805476, whole genome shotgun sequence.
ACCESSION   DATIFQ010000121 DATIFQ010000000
VERSION     DATIFQ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805476"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VLE22_05390"
     CDS             <1..371
                     /locus_tag="VLE22_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13870.1"
                     /translation="LWLTTIPWNLLWNLLARRYRPRPPLIRIVRTTVQVLVVGLLVHL
                     AEVLAVLCRYVFRRSDPFRKTQQILYEYAQTPSPAGVPVLEGRAAEAAAASENGSAQR
                     RTLAGKERSLRPPCLRLRRW"
     gene            535..2319
                     /locus_tag="VLE22_05395"
     CDS             535..2319
                     /locus_tag="VLE22_05395"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HSB13871.1"
                     /translation="MFLRRHRRTKDGKPHSYWSLVETVRTPDGPRQRRLCYLGELNES
                     AQARWLKGIEVFNAQGESRQLKLFPSEVEPPEDDPAVARVRVDKVRLERPRRFGDCYL
                     GLELWRRLALDHFSESVLDRLEDPADVPWSRTAALLVINRLCAPGSELAIEERWYPTT
                     ALDDLLHIGPGKINDTRLYRCLDRLIPHKTKFERHLVERYGELFAAQYDVLLYDLTSS
                     YVEGAAEKDPMMQRGYSRDHRPDCKQVVIALIVNVEGFPLSYETFDGNRADVTTLEAV
                     LRMVERKYGKARRVWVFDRGIISEENLEALRRRGGQYLVGTPRAKLKQFEQPLLESSG
                     WEQVRPEVEVKLVPTPQGEETYVLCRSTARREKELAIRSRFSQRLEKGLKALAKRVAE
                     GKLKDRGKVERRLGSLVARHPQVADLYAAKITERAGRLQLDWNLRPERRAWQQAREGA
                     YLLRTNLPATEPEQLWKSYIQLTEAEAAFRALKSELSIRPIFHQLERRAKAHILVAFL
                     GYALWVTLKHLLIRKRSNLSPGKALHLLSTLQSADIILPTTDGREIRLRRITTPSREQ
                     QLLLDQLGICLPERLSFDQECSADSAVA"
     gene            complement(2336..>2975)
                     /locus_tag="VLE22_05400"
     CDS             complement(2336..>2975)
                     /locus_tag="VLE22_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HSB13872.1"
                     /translation="ATRWHQLERWHKETLAVADNDESLDLLKVRTRRGDFWVQRNGTH
                     WDGTQLLAYLLTEHQMFAEKASAGYVRRGDVVVDCGAHIGVFTRFALDAGASRVVAIE
                     PDPANLECLRRNMAGDSRVTVIPKAVWSEETGTLELLIAANNSGSNRVVGSHGANTIR
                     VAATTLDNIEKDLRLTRVDFIKLDIEGAERHALKGASRMLSPSSSREGRGLS"
BASE COUNT          564 a          910 c          942 g          559 t
ORIGIN      
        1 tcttgtggct gaccaccatt ccctggaatc tgttgtggaa cctgttggcc cgtcgctatc
       61 gtccgcgccc gccattgatt cgtatcgtcc gcacgaccgt ccaggtgctg gtagtcgggc
      121 tgctggttca tctggccgag gtgctggcgg tgctgtgccg ctatgtgttc cggcgttccg
      181 atcccttccg aaagacccaa cagatcctct acgaatacgc acaaaccccg tcacccgcag
      241 gtgttccggt cctggaaggt agagcggctg aagcagcagc agcaagcgaa aacggctccg
      301 cgcaaaggcg cacgctcgca gggaaagaac gctccctccg tccgccctgt ctccggttga
      361 gacgctggtg agacactcta gcccaagttc gtcacggcgg ggttgatttc gggcattttc
      421 gggcgtgggc ggagcagaag tggtgtggct gcaacgcggg tgctaccgaa agccgatgta
      481 gtgcagacct accctcttga agttgcgcaa cagccgtgac aagatcgccg cagaatgttt
      541 ctgcggcgac atcggcgcac caaggatggc aagccacaca gttactggtc gctggtggaa
      601 acggtgcgca cgccggacgg gccgcggcag cgcaggttgt gttatctggg cgaactgaac
      661 gagtcggcgc aggcgcggtg gctgaagggc atcgaggtgt tcaacgccca gggcgagagc
      721 cggcaactga agttgtttcc ctccgaggtt gagccgcccg aggacgatcc cgcggtggcg
      781 cgggtgcggg tggacaaggt gcggctggag cgcccgcgcc gtttcggcga ttgttacctc
      841 ggcctggagt tgtggcggcg cttggctctc gatcatttct cagaaagcgt gctggaccgg
      901 ctcgaggatc cggccgatgt gccctggtcg cggaccgcag ccttgctggt gatcaaccgc
      961 ctgtgcgcgc cgggcagtga gctggccatc gaagagcgct ggtacccaac cacggcactc
     1021 gacgacttgc tccacatcgg gccgggcaag atcaacgaca cgcggctcta ccgctgcctg
     1081 gatcggctca tcccgcataa gacgaaattc gagcgacacc tggtcgagcg ttatggcgag
     1141 ttgtttgccg cccagtacga cgtgctgctc tatgacctga ccagcagcta tgtggaaggt
     1201 gcggccgaga aggacccgat gatgcaacgc ggttactcgc gggatcatcg gccggactgc
     1261 aagcaggtgg tgattgccct catcgtcaac gtcgaaggct ttccgctgag ctacgagacc
     1321 ttcgacggca accgggccga cgtgaccacg ttggaagccg tgctgcggat ggtggaacgc
     1381 aagtacggca aggcgcgccg ggtatgggta ttcgatcgcg gcatcatcag cgaggagaat
     1441 ctggaggctc tgcggcgccg tggcggccag tatctggtag ggacgccacg ggcgaagctg
     1501 aagcagttcg agcagccgtt gctggaaagc agtggctggg agcaggtgcg tcccgaggtg
     1561 gaagtgaaac tggtgccgac accgcagggg gaggagacct atgtgctgtg tcgctccacc
     1621 gcccggcggg agaaggaact ggccatccgc agccgcttct cgcagcgtct ggagaaaggg
     1681 ctgaaagctc tggccaagcg ggtggcggaa ggcaagctca aggaccgcgg caaagtggag
     1741 cggcgactgg ggagcctggt ggcgcgccat ccgcaggtgg ccgatctgta cgccgcaaaa
     1801 atcacggagc gtgccggacg cctccagctg gactggaatc tgcggccgga gaggcgcgct
     1861 tggcagcagg ctcgcgaagg cgcctacctg ttgcgcacca atctgccggc caccgagcca
     1921 gaacaacttt ggaagagcta tatccagctc acggaagcgg aggcggcctt tcgcgcgctc
     1981 aaaagcgagc tttccatccg ccccattttt caccaactgg agcgccgcgc caaggcgcac
     2041 atcctggtgg ccttcctggg ctacgccctg tgggtcacct tgaagcatct gctgatccgg
     2101 aagcgctcga acttgtcccc gggcaaggcg ctccacctgc tgtcgacgct gcaaagcgcg
     2161 gacatcatcc tgcccaccac cgatggccgc gagattcgtc tccgccggat caccacaccg
     2221 agtcgtgagc aacagttgct tctcgatcaa ctcggcatct gccttcctga gcgcctcagt
     2281 ttcgatcagg aatgtagtgc agactcggcg gtagcctgaa ctgattcgaa aagacttaac
     2341 tcaatccccg gccttcccgt gacgaacttg ggctaagcat ccgcgaagct cccttcaaag
     2401 catgccgctc cgcgccctcg atatcgagtt taatgaagtc gacgcgtgtc agcctgagat
     2461 ctttttcgat attgtccaac gtcgtcgcgg cgaccctaat ggtattggca ccgtgcgagc
     2521 cgacgactcg gttgcttcct gagttgttcg ctgcaatcag caactcgagt gtcccagtct
     2581 cctcactcca taccgccttc gggattacgg ttacacgact gtcacccgcc atgttccgcc
     2641 gcaggcactc caggttcgcc ggatcgggct ctatggcgac gacccggctg gcgcccgcgt
     2701 cgagcgcgaa ccgggtgaac actccgatat gggcgccaca gtcgaccacc acgtccccgc
     2761 ggcgaacgta gccggcgctc gccttctcag caaacatctg gtgttcagtc aagagatagg
     2821 caagcagttg agtgccgtcc caatgtgtac cgttacgctg gacccagaag tccccgcgtc
     2881 gcgtacggac cttcagcagg tcaagcgatt catcattgtc ggccacggca agtgtttcct
     2941 tgtgccatcg ctcaagctga tgccatctcg tggca
//
LOCUS       DATIFQ010000122        17118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_997690, whole genome shotgun sequence.
ACCESSION   DATIFQ010000122 DATIFQ010000000
VERSION     DATIFQ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17118
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997690"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1730)
                     /locus_tag="VLE22_05405"
     CDS             complement(<1..1730)
                     /locus_tag="VLE22_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13873.1"
                     /translation="MTKEEIRLQESRERRAHWNRWGPYLSERAWGTVREDYSPGGTAW
                     EYLPHDHARSRAYRWNEDGLAGISDRHQLICFAIALWNGRDPILKERLFGLTGNEGNH
                     GEDVKEQYFYLDSTPTHSYMRYLYKYPQAEFPYAELVAGNRARDKHSREYELLDTGVF
                     NESRYFDVFVEYAKGSPEDILIRITAANRGPEPATLHLLPTVWFRNTWSWGNGGERPS
                     LRQVCSCSIELEHTTYGRRRLVCEGSPEMLFTENETNYQRLYGGERKYAKDGINDCIV
                     QGHTDVVNPTRTGTKAAYHYVLHLGSGETAAVRLRLTDLAVDRLDESFDRIFAERQRE
                     ADEFYATVIPKDLSDDGRNVMRQSFAGLLWSKQFYHYVVRDWIKGDPAFPSPPPERRA
                     GRNHEWTHLYNADVISMPDKWEYPWYAAWDLAFHCVPLALVDSDFAKEQLILMLREWY
                     MHPNGQLPAYEWAFGDVNPPVHAWAAWRVYKIEKRRRGVGDRVFLARVFQKLLLNFTW
                     WVNRKDAEGRNVFQGGFLGLDNIGVFDRSAPLPTGGHIEQADGTGWMGMYCLNLLAIA
                     MELAREESCY"
     gene            complement(1760..2398)
                     /locus_tag="VLE22_05410"
     CDS             complement(1760..2398)
                     /locus_tag="VLE22_05410"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HSB13874.1"
                     /translation="MRIRLSVLLAIATGLLHAEVAAYNIRPAQGARFVLTVEKTGLLR
                     GKKHQFVFEDYKGTLHYDSDSPERSRVDLSIRSASAVCMDKWVSQKDLKKIQAYALDD
                     MLAAEKYPELHFSSAGVVRKGENTFEVAGTLTIRGIAKPTTVMVELGRDSGRISSLSG
                     HAVVRLKDYGLKPPTAALGTIGTKNEMTVEFSLVPVAAIETALSGARSATEP"
     gene            complement(2402..3022)
                     /locus_tag="VLE22_05415"
     CDS             complement(2402..3022)
                     /locus_tag="VLE22_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB13875.1"
                     /translation="MKGTEAIAVVAMLMAAPGALAQRHEIGLTLGAISGADRNTSSGQ
                     LGLGTGVALQANYGYRIFASRHAALFAEVHFLANPLRDITSMDGRASRDVATLYVLPG
                     LRIKFLPARSFSPYVAVGGGYALYEQSLTQIDGSPNPAPRNTHRGALGFGGGADFPVW
                     RFVGGRIEIRDFYTGNPSFNIPVSGGGQHNVVAGGGVVLRLGSSEK"
     gene            complement(3080..3826)
                     /locus_tag="VLE22_05420"
     CDS             complement(3080..3826)
                     /locus_tag="VLE22_05420"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HSB13876.1"
                     /translation="MEPYRIRCSEIWGGVRSIDFDVRTGGLTASLYSQACGGDAGGDI
                     YYFSVCGSDSLTRIALADVRGHGEQVSHMSSWIYDSLKQKMNTLDGGGVLCDLNAMIH
                     QHGFEALTTASVVGYYATESRLYFSYAGHPPALFCRRTEGKWGPLCLSSSGRSRNTIL
                     GALPSVHFDQEHTSLVPGDRFFLYTDGVIECPDLRGDEFGQQRLIALLNDADGESLVE
                     IKTRVVEAIIQHIAPGPFNDDFTLLAAEVL"
     gene            complement(3882..4331)
                     /locus_tag="VLE22_05425"
     CDS             complement(3882..4331)
                     /locus_tag="VLE22_05425"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HSB13877.1"
                     /translation="MRNLLTMIAAAGLLIVAPTSGRSLVDRVNKNGQGVALKGYDPVA
                     YFEQGRPVKGSPKFPHEWMGATWWFASAENRALFTKDAQKFAPQFGGYCAYAVSEGYT
                     ANIDPEAWKIVDGKLYLNNSKGVQKKWKQDVQTRIGAAEKNWPSLHK"
     gene            4440..5243
                     /locus_tag="VLE22_05430"
     CDS             4440..5243
                     /locus_tag="VLE22_05430"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSB13878.1"
                     /translation="MARLSGKNVLVTGSSSGIGQAIAVRFAQEGANVAINYRSGAEQA
                     EVTRAMAQKARTNGAGKEFVVQADVSIEDQVKTMFAKVIEAFGSLDVLVNNAGIQKPS
                     PSHEVETSDFDRVIAVNLRGPFLCAREAIRHFLARGGGGVILNNSSVHEIIPKPKYLP
                     YSVSKGGIENLTRSLALEYAGDGIRVNAVGPGAVVTPINRAWIDNPTARAQVESHIPM
                     NRAAAADEIAAVFAFLASDEASYITGQTIFACGGLTLYPEFRVAWSSGE"
     gene            5254..5952
                     /locus_tag="VLE22_05435"
     CDS             5254..5952
                     /locus_tag="VLE22_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSB13879.1"
                     /translation="MNQLENKVTLITGAKGGLGTSVTQAFLSSGATVVGSSRSIQISD
                     FAHPNFTAIPAELSSVEAARSLADSVLARHGRIDVLVHVVGGFEGGKPVAETEDATLD
                     HMLDLNLRSAFHITRAVIPHMRKQAGGRIVVVGSRAAVQPQALVGAYSASKAAMLSLM
                     QTLALENVDLGITVNVVLPGTMDTAAARASDPKADFSKWVPPAKVASTILWLVSDDSS
                     HVSGSAIPVYGRSL"
     gene            5969..6229
                     /locus_tag="VLE22_05440"
     CDS             5969..6229
                     /locus_tag="VLE22_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13880.1"
                     /translation="MAIAEAFVVALAAYMIIGVAFAIPFLLVAVQRIDAQARGAGVGF
                     QLLILPGVAALWPLFLRRWIHGLSEPPVERNPHRKAALTGGS"
     gene            6229..6696
                     /locus_tag="VLE22_05445"
     CDS             6229..6696
                     /locus_tag="VLE22_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13881.1"
                     /translation="MIQPLRTGHRLVMFTLAAALPVLFVAGLVARKSPTPANQAVRWE
                     GDGGSQVQWQSATPFRLGSTEATIRESADGRWLEIRLSADPLTPDLLVYWSAIAPSGD
                     ALPPDSHLLGTLAGPRAKRMQLPPSARQTSGYVMVYSLAHQSLAGSVRLRGLR"
     gene            6693..7751
                     /locus_tag="VLE22_05450"
     CDS             6693..7751
                     /locus_tag="VLE22_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSB13882.1"
                     /translation="MSVQYQAIGWNRQKRVYDTILAGSVLLYLALFVGFGTLIHANAT
                     AETLLIRAFGTAALLLLHVVLSIGPLCRLDRRFLPLLYNRRHLGVTTFLLGLAHGGFA
                     IFQFHSIGNVNPLVSLLISNRRFGSLADFPFQLLGFLALLILFLMAATSHDFWLRNLT
                     APIWKRLHMLVYLAYALLVAHVTLGALQSETNLVFAAILGAGMVTVLSLHVAAAVRER
                     RVDSSVVRANEDGFVYVCDVESIPEKRARIVSLSGERVAVFRYDGKVSAISNVCQHQN
                     GPLGEGRIIDGCVTCPWHGYQYLPDSGASPPPFTEKVPTFRTRVAGGKVFVHPRPHAP
                     GTRLEPARIDGQTERVAV"
     gene            7748..8509
                     /locus_tag="VLE22_05455"
     CDS             7748..8509
                     /locus_tag="VLE22_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13883.1"
                     /translation="MNNDEFYVGYAPKPPEHLRRTIVKIVIALNAIAVVLAVLLVLGQ
                     QHFAASAFEFQQFREFEGVLLEHPYPSLLVRRPGMAEGTLPFSRYLLVASGKHAASSV
                     VSGLDGRKVRIRGSLIYREGNTMIELASGAAPVAEEADSNRAATASDLGQVTLSGEIV
                     DSKCYLGVMNPGSGKVHRDCAVRCISGGIPPAFSVKDAEGRPKILLLAGVDGRQLNRE
                     VLDFVAEPITIRGRLTRSGETYILWTEPSSFVRRH"
     gene            8556..10460
                     /locus_tag="VLE22_05460"
     CDS             8556..10460
                     /locus_tag="VLE22_05460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HSB13884.1"
                     /translation="MTEPTIDAVLRNLFPVPIPKPVDGALGRILGLRQAANLYRDLIA
                     ERDDRPLTDRLLSHLHVTYRVSDRDLDRIPRLGPVVIVANHPYGILDGAVLASILLRL
                     RPDVKFLANQMLSVIPEIRDLLIPVDPIGGAASTQINGRGVKQALDFLSGGGLLVVFP
                     AGEVSHFRWKERSVADPEWNPAISRVIAIACRRVRGFSVVPAYVSGSNSFLFQTLGIV
                     HPRLRTALLVRELLNKRRHDVEVRIGHPLLGERLLAIPTDGQRTQHLRWRANLLACRN
                     PFKAKTASSLRSRRRRETQEPIAAAMPAEGLTREVQALGALLKSSEFEVYLAPADRIP
                     AVLAEIGRLREVAFRAAGEGTGKASDLDAFDAHYLHLFVWNTDRHEVVGAYRLGPVDS
                     IRRRLGIAGLYTATLFNYGDEFLDRLGPALELGRSFIRPEYQRAFAPLLLLWKGIGKY
                     IAENLRYKVLFGPVSISNQYQSVSRQLIVSFLECHAALNEWAALVSSKNPFRSRAARP
                     WKGEGYTSPGLDIEDLSAAVADIEPAQPGVPVLLRQYLKLGGKLLGFTVDPEFADSLD
                     GLIMVDLTRTELKLLERYLGKVEAAEFLNFHWGEYDPQQTSHTSELDAASHRSGDSAA
                     NAVLQVHRRG"
     gene            10414..10788
                     /locus_tag="VLE22_05465"
     CDS             10414..10788
                     /locus_tag="VLE22_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13885.1"
                     /translation="MILLQTLFFKFTGAAESVYIFTTLGAEPWGRIGSGVVELIASIL
                     LLVPRTVVYGAILSLGTMGGAIFSHLTKLGVALPAVDDHGELFALAVLVFVCSAAVLF
                     LHRHELPLVRHRAVTPVAGRLG"
     gene            complement(10821..11147)
                     /locus_tag="VLE22_05470"
     CDS             complement(10821..11147)
                     /locus_tag="VLE22_05470"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HSB13886.1"
                     /translation="MREYSILGIEYLAIAVEIAAAAIITLAVAEAAVRAAPLFVRRNT
                     PQEAKVDLRLALGRWLAVGLEFALAADILRTAIAPTWNDIGQLAAIAVLRTALNFFLA
                     KEIERV"
     gene            complement(11140..12774)
                     /locus_tag="VLE22_05475"
     CDS             complement(11140..12774)
                     /locus_tag="VLE22_05475"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSB13887.1"
                     /translation="MPHLILIALATLVSEDLTCIATGVLVAQGKVGFISGILACLAGI
                     YFGDLLLFLGGRCIGRPVIRWPLFRRFVSPEQLDRASSWLSSKGAAVVLLSRFTPGLR
                     LPTYFAAGLLRTRVWRFSMYFLLAAAIWVPLVVCLTVALGGPVVRTMLFAGGKSSMAI
                     VAIFVLWIGLRQELPLLFGFRTRRKLAGFLKRKVRWEFWPPWAAYLPLIPYLALLALK
                     HRSPTLFTAANPGMPSGGFIGESKSEILDHLDRIPGIVAAYTRIPGSLESTARIRKAN
                     AFLQQHGLAFPVVLKPDVGERGSGVAIIRSLGEMESYLEAANGDTILQEYIEGYEFGV
                     FYYRYPLEPTGHILSITKKRFPEVIGNGRNTLEELVLKDDRAVCLASTYLGRIGRSAH
                     EVPAPGEAVRLAELGSHCRGAVFLDGSRLITQPLEAAIDRISKSHPGFFFGRFDIRAP
                     SIDAFKEGQPLKVIELNGVTAEATHIYDPAVSLLDAYRTMLTQWRIAFEIGATNRRLG
                     ANPMTVNEFIRLLWKRVRREGGFSEQMAPNRRVIHA"
     gene            complement(12762..13481)
                     /locus_tag="VLE22_05480"
     CDS             complement(12762..13481)
                     /locus_tag="VLE22_05480"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HSB13888.1"
                     /translation="MSWVHEDEGYQLFCNRDERISRAPALGPRIAARAGVRFVAPIDG
                     DFGGAWIGVNEFGVSLCLLNGAPRLAGRVPAPHRSRGLLVLELLSATCLAEINERVCR
                     QDLSPFTPFTLAALEPGLPAAVLEWTGEETAIVPYGDPYMPLVSSLFDQTGVLASRRE
                     ELARTAGSAARLDSDVLHAFHKSHGDRPSAYSPCMHRADAETVSFTHVRVTESAVTLT
                     YTPAAPCRRLPGERVTLPCRT"
     gene            complement(13478..14002)
                     /locus_tag="VLE22_05485"
     CDS             complement(13478..14002)
                     /locus_tag="VLE22_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13889.1"
                     /translation="MTLTESSTVLTETSVRLLHQVKSLLEGMDSRAYAESPPAVTPHR
                     VGGHLRHILEFYECFLDGVGSGRIDYDARKRDITVETDCRAALAKTREIMLRLLIGDF
                     ESERVVNVRMEDGDSFLASSVGRELQVLSSHTVHHFALIALVLKALGIPVEHDFGMAP
                     STLRYLAEASKCVR"
     gene            14088..14921
                     /locus_tag="VLE22_05490"
     CDS             14088..14921
                     /locus_tag="VLE22_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HSB13890.1"
                     /translation="MRTSTASAQQTALRGEWYEALADHWPEYLMEGAALGLFMISACV
                     FTVLLEHPSSWLHQAIPSPVLRRLLNGVAMGCTAIGLIYSPWGKRSGAHMNPSVTLTF
                     LSLGKIQRWDAIFYVTAQFLGGLFGVVLAAMSIGLPLRHSAVNYVVTVPGTGGPGIAF
                     WAELLISFLMMWMILFASNTKRFERFTGVFAGFLLAAFITFEAPLSGMSINPARTLGS
                     AIPANEWTALWVYFAGPPLGMFLASAAYRLRWGAHAVFCAKLHHHNDKRCIFRCNYAA
                     M"
     gene            14951..16522
                     /locus_tag="VLE22_05495"
     CDS             14951..16522
                     /locus_tag="VLE22_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HSB13891.1"
                     /translation="MSSSNHYDVIVIGTGAGGGTLAYKLAPAGKRILLLERGDYVPRE
                     KDNWNPRVVNVEGKYQTKEIWKDSDGKDLHPHTNYWVGGNTKFYGAALFRLRKEDFGE
                     LRHHGGVSPAWPISYDELEPYYTEAERLYHVHGQRGEDPTEPPARTPYPHPAVSHEPR
                     IEQLSEDFARNGATPFHTPLGVMLDEASRHTSPCIRCNTCDGFPCLVHAKSDAQVVAV
                     DPALAHPNVTLLRNSFVERLETSPSGREVTSVIVTRNGAREAFSADIVAVSCGAINSA
                     ALLLRSASDKHPGGLANGSGVVGRHYMGHVNSVLMAISKCPNPTVFQKTLSVNDFYFG
                     SKDWEYPMGHISFVGKLDGETLKAGAPAIAPGFTLDLMASHSLDFWLTSEDLPDPNNR
                     VTLDREGNIVLAYKPNNEEGHKRLIAKLRDLMNQQRKCPMHGHECHQGLFARSLYLGQ
                     RIPLAGVAHQNGTIRFGRDPKTSALDANCKAHDLDNLYIVDASFFPSSGAVNPGLTIM
                     ANALRVGAHLIERMG"
     gene            16526..>17118
                     /locus_tag="VLE22_05500"
     CDS             16526..>17118
                     /locus_tag="VLE22_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB13892.1"
                     /translation="MHRRAFLILVLLASTLCANDVSPLVTAVDSIGLTVSDLDRSVEF
                     YAKVLSFEKVSEGEVAGEDFEHLHGVFGLRLRTARMRLGDEFIELTEYLAPRGRPAPA
                     DARSNDRWFQYIAIIVSDMHKAYQWLRQNKVQHSSPGPQRLPDWNRNAGGIEAFYFRD
                     PDGHPLEILAFPPDKGDPKWRRSADKLFLGIDHTAIVV"
BASE COUNT         3311 a         5198 c         5118 g         3491 t
ORIGIN      
        1 tcgtaacacg attcctcgcg agccagctcc atcgcgatcg cgaggagatt cagacagtac
       61 atccccatcc agcccgtacc gtccgcctgc tcgatgtgtc ctccagttgg gagcggagca
      121 cttcggtcaa atacgccgat gttgtcgagt ccgagaaagc cgccctggaa gacgtttcgt
      181 ccttcggcgt ccttgcggtt cacccaccag gtgaagttca gcagcaactt ctgaaagacg
      241 cgcgccagga acacacggtc gccgacgccc cggcgccttt tttcgatctt gtagacgcgc
      301 caggcagccc aggcatgcac cggcgggttc acgtcaccga acgcccactc gtacgcaggc
      361 agttgaccgt tcggatgcat gtaccattcg cgcagcatca gaatgagctg ttcctttgcg
      421 aagtccgaat cgacgagggc aagcggcacg cagtggaagg cgaggtccca ggcggcgtac
      481 cacggatact cccacttgtc cggcattgaa atgacgtcgg cattgtacag gtgagtccac
      541 tcgtggtttc ggcccgcacg ccgctccggc gggggtgacg ggaaagcagg gtcgcccttg
      601 atccaatccc taaccacgta atggtaaaac tgcttggacc aaagcagccc ggcaaacgac
      661 tggcgcatca cattcctccc gtcgtcggaa agatcctttg ggatcacggt cgcatagaat
      721 tcgtcggctt cgcgctggcg ctcggcgaag atccggtcga agctctcgtc aagcctgtcc
      781 accgcgagat ccgtgagccg caggcgaacc gccgctgtct cccccgagcc gagatgcaga
      841 acgtagtgat aggcggcctt cgtgcccgtc cgggtgggat tcaccacatc cgtgtggcct
      901 tgaacgatac agtcgttgat tccgtccttg gcgtatttcc tctcaccgcc atacaatcgc
      961 tggtagttcg tttcgttctc ggtaaagagc atctccggcg agccttcgca gacaagccgt
     1021 cttcggccgt atgtcgtatg ctccaattcg atcgagcaac tgcacacctg gcgcaggctc
     1081 ggacgctcgc ctccatttcc ccatgaccac gtgttccgaa accacacggt gggcaaaaga
     1141 tggagcgtgg cgggctcggg accacgattg gcggcggtaa tccggatcag gatgtcctcc
     1201 gggctccctt tcgcatattc cacgaacaca tcgaaatagc ggctttcgtt gaacacgccg
     1261 gtatccagga gctcgtactc gcggctatgt ttgtcgcgtg cgcgattccc ggcaaccagt
     1321 tccgcgtagg ggaactcggc ctgcgggtac ttgtagagat accgcatgta ggaatgcgtc
     1381 ggcgtgctgt cgagatagaa atattgctcc ttgacatcct cgccgtgatt tccttcgttg
     1441 ccggtgagcc cgaacaatcg ctctttgaga atcggatcgc gtccgttcca cagtgcgatg
     1501 gcgaagcaga tcagttggtg gcgatcggag attccggcaa gcccatcttc gttccaccgg
     1561 taggcccgtg agcgggcatg gtcatgtggc aggtattccc aggcggtgcc tccgggactg
     1621 tagtcctcgc gcaccgtgcc ccaggcacgc tcgctgagat atgggcccca gcggttccag
     1681 tgagcgcgtc gctcacgaga ttcctgtagc ctgatttctt ccttcgtcac ggtgtgtgtc
     1741 ctttcccctt tgcctttgtt cagggctctg tcgcggagcg tgcccccgag agagctgtct
     1801 caatggcggc taccggtaca agggagaact ccactgtcat ttcgttcttg gtcccaatcg
     1861 tgccgagagc cgccgttgga ggttttaggc catagtcctt gagccgcacc acagcgtggc
     1921 ccgagagtga tgagattcta cctgagtctc tacccagttc aaccattacc gtcgtgggct
     1981 tggcaatccc acggatcgtc agcgtgcccg cgacttcgaa cgtgttctcg cctttgcgca
     2041 cgaccccagc ggaggaaaaa tgcagctccg ggtacttctc agccgctagc atgtcgtcga
     2101 gcgcataggc ctggatcttc ttcagatcct tttggctgac ccacttgtcc atgcacaccg
     2161 cgctcgccga tcggatggac aaatcgacgc gcgaacgctc gggcgagtcc gaatcgtaat
     2221 ggagcgtgcc cttgtaatct tcaaaaacga actggtgctt ctttcctctt aacaggcctg
     2281 tcttctccac cgtcagaacg aatctggctc cctgcgcggg gcgtatgttg taggctgcaa
     2341 cttctgcgtg caacaacccc gtcgcgattg ccagcagtac gctcagacga atccgcatcg
     2401 gtcacttctc cgaacttccc agacgcagaa cgacgccacc gcccgctacg acgttgtgtt
     2461 ggccgcctcc agagaccgga atgttgaagc tcggattgcc cgtgtagaaa tcccgaatct
     2521 caatgcggcc accgacaaaa cgccacacgg gaaaatccgc gccgcctccg aatcccagag
     2581 cgccacgatg ggtgttccgt ggcgccggat tgggactgcc gtcgatttgc gtcaagctct
     2641 gttcgtatag cgcgtagccc ccacctaccg ccacgtaagg tgagaatgaa cgcgctggca
     2701 gaaacttaat tcgcagccca ggcagaacgt aaagcgtcgc gacatcgcgg ctggcccgcc
     2761 cgtccatgga ggtaatgtcc ctcagcgggt tcgcaaggaa gtgcacctcg gcaaacaaag
     2821 cagcatgccg cgaggcgaag atgcgatatc cgtagttcgc ttgcaatgcg accccagtcc
     2881 cgagacccag ctgtcccgaa gaagtgtttc gatccgcgcc cgagatggcg cccagagtaa
     2941 gaccgatctc atgccgttgg gcgagcgcgc ccggtgcagc catgagcatc gccacaaccg
     3001 caatcgcttc cgtccctttc attgtcgtac ctcaccttga catcccgcca gttctgcccg
     3061 gtattcccac tcccagcact caaagtactt cggcagccag cagagtgaag tcgtcgttga
     3121 agggcccggg cgcaatgtgc tggatgatcg cttccacgac tcgcgtcttg atctcgacaa
     3181 ggctctcccc gtcggcgtcg ttaagcaatg cgatgaggcg ctgctgaccg aactcgtcgc
     3241 cacggagatc cgggcactcg atcactccat ccgtgtaaag gaagaagcga tcgcctggca
     3301 caagagaggt gtgttcctgg tcgaaatgca ccgaaggaag cgctccaaga attgtgtttc
     3361 gagacctgcc gctggaactg aggcaaagcg gaccccactt gccttccgtc cggcgacaga
     3421 acaacgcagg tgggtgccca gcatacgaga agtacagacg cgactcggtt gcgtagtaac
     3481 cgaccacgct tgctgtcgtc aaagcctcaa agccgtgctg gtgaatcatt gcattaaggt
     3541 cgcagaggac gccgccgcca tcgagcgtat tcatcttctg tttcagggaa tcgtagatcc
     3601 aggaactcat gtggctgacc tgctcgccgt gaccccggac atccgccagc gcgattcggg
     3661 tcagggaatc cgagccgcag acgctgaagt agtagatgtc cccgccagca tctccaccac
     3721 aggcttggga gtacaagctg gctgttagtc cgcccgttcg gacgtcgaag tcgatactgc
     3781 gcacaccgcc ccagatctcg gagcatcgga ttcggtacgg ttccatcggg cccccttcgc
     3841 agcagacact cactcggcgc gggcgtcgga attcccgcgg tctatttgtg cagtgacggc
     3901 cagttcttct cggctgcccc gatccgcgtc tggacgtctt gcttccactt cttctgcacg
     3961 cctttactat tattcaggta gagcttgcca tcgacgattt tccacgcctc cggatcgata
     4021 ttcgctgtgt acccctcgct gacggcatag gcgcagtagc cgccgaactg cggagcgaac
     4081 ttctgcgcgt ctttagtgaa gagggcgcgg ttttcggcgc ttgcgaacca ccacgtcgct
     4141 cccatccact cgtgtgggaa cttcggtgac cccttcacgg gccggccttg ctcgaaataa
     4201 gcgaccggat cataaccctt cagagcaacg ccttggccgt tcttgtttac ccggtcaacc
     4261 agcgaacggc cggacgttgg ggccacgatt aaaaggccgg cggccgcaat cattgttagg
     4321 aggtttctca tgccttgttc atcctcgcca cggtcagggt tattcccttc gggccgcgga
     4381 atagcggaat aaacggacgg caccgacgga tgttgctgtt gaagttcgag aggtagcaaa
     4441 tggcaagact gagtggaaag aacgttctgg tcacggggtc gtcctccggg atcggtcagg
     4501 caatcgcggt ccgattcgct caggaaggcg caaatgtggc gatcaactat cgaagcggcg
     4561 ctgagcaggc tgaagtcacc agagcaatgg cgcaaaaggc tcgcaccaat ggagccggca
     4621 aggaattcgt ggttcaggcc gacgtgtcga tcgaggacca ggtcaagacc atgttcgcga
     4681 aggtgatcga ggccttcgga tcactggacg tcctcgtcaa caacgccggg attcagaaac
     4741 catctccgag ccatgaggta gaaacaagcg actttgaccg cgtcatcgcg gtcaacctgc
     4801 gtgggccgtt cctttgtgcg cgcgaggcga tccggcactt cctggcccgg ggcggaggcg
     4861 gcgtgatcct gaacaactcg agcgtccacg aaatcatccc caagcccaag tacctcccct
     4921 attcggtcag caagggtgga atcgagaacc tgacaaggtc gctggcgcta gaatacgcgg
     4981 gagacggcat tcgtgtaaac gcggtgggcc ctggcgcggt agttacgccc attaaccgcg
     5041 catggatcga caatccgaca gcgcgcgcac aggtggagag tcatatcccg atgaaccgcg
     5101 ctgcggcggc ggatgagatt gcggcggtgt tcgcgttcct cgcctccgac gaggcctcgt
     5161 acatcaccgg gcaaacgatc tttgcttgcg gcggactgac cctgtatcca gagttccgcg
     5221 tcgcctggtc ctccggcgag tgaaggagac ttcatgaatc agcttgagaa caaagtgacg
     5281 ttaatcaccg gcgcgaaggg cggcctcggc actagcgtta cccaggcgtt cctgagcagc
     5341 ggcgccaccg tggtcgggtc gtcacgttcc atccagatat cggatttcgc tcatcccaat
     5401 ttcacagcga tcccggccga actgtcgagt gtggaggccg cgcgatcatt ggccgattct
     5461 gtgctcgcgc gtcacgggcg aatcgacgtc ctcgttcacg ttgtcggtgg tttcgagggc
     5521 ggtaagcccg tagctgagac cgaggacgcg acgctcgatc acatgctgga cttgaatctg
     5581 cgatcggcat tccacatcac tcgcgccgtg atcccacata tgcgcaagca ggccgggggg
     5641 cggattgtgg tcgtgggtag ccgtgcggcg gtacagcccc aggcgcttgt gggggcgtac
     5701 agtgcatcta aggcggcgat gttgtccctt atgcaaacgc tggcgctcga gaacgtggac
     5761 ttggggatca cggtgaacgt cgtcctgccg gggacgatgg acacggcggc ggcccgcgcg
     5821 agcgatccca aggctgactt ttcgaagtgg gttccaccgg ccaaggttgc aagcacgatc
     5881 ctgtggctgg tgtcggacga ctcgtcgcac gtgagcggct ctgcgatccc ggtgtacggc
     5941 cgcagcttat agcgccacgg agggagcaat ggccatcgct gaagcgttcg ttgttgcgct
     6001 cgccgcgtac atgatcatag gcgtggcgtt cgcgattccg ttcctgctcg tggcggtgca
     6061 acggatcgac gcacaggctc gaggcgcagg ggtcggcttt caattgctca tcttgccagg
     6121 agtcgcggct ttgtggccct tgttcttgag gcgctggatc cacggtctgt ctgagccgcc
     6181 cgtcgagcgc aaccctcacc gaaaggccgc actgacggga ggctcgtgat gatccagccg
     6241 cttcggaccg gacacaggct cgtgatgttc acacttgcgg cggcactgcc ggtcctgttt
     6301 gtcgcgggct tggttgccag gaaatcgcca actcccgcca accaggccgt cagatgggag
     6361 ggagacgggg gctcacaagt ccagtggcaa tctgcgactc cgttccggct cgggagcacg
     6421 gaggccacca ttcgcgagag tgctgacgga agatggctcg aaatccggct cagcgctgat
     6481 ccgctgacgc ccgatctgtt ggtctactgg tcggccatcg cgccgtccgg cgacgcgcta
     6541 ccgcctgaca gccacctgct cggcacactg gccggcccgc gtgccaagag gatgcagcta
     6601 cccccgtccg cccggcagac tagcggatac gtgatggtct acagtctggc gcaccaaagc
     6661 ctagccggca gcgtgcgttt gaggggcctt cgatgagcgt ccagtatcaa gctatcggat
     6721 ggaatcgtca gaagcgcgtt tacgacacca tcctcgccgg ttcggtgctg ctttacctcg
     6781 cgctgtttgt cggatttggc acgctgattc acgccaatgc gacggcagag acgttactga
     6841 tccgcgcctt tggcacggcc gcgctcctgc tcctgcacgt agttctttcg atcgggccgc
     6901 tatgccggct cgatcggcga ttccttccgt tactctacaa ccgcagacat ctcggcgtga
     6961 cgacgtttct gcttgggctg gctcacggtg gattcgcgat cttccagttt cacagcatcg
     7021 gcaacgtaaa cccgctggtg agcttgctca tcagcaaccg gcgctttggc agtctcgccg
     7081 actttccttt tcagttactc ggcttcctcg ccctgctcat ccttttcctt atggcggcaa
     7141 ccagtcacga cttttggttg cgcaacctga cagctcccat ctggaagcgg ttgcacatgt
     7201 tggtgtatct cgcctacgct ctgctcgttg cgcacgtcac gctgggagct ctccaatcgg
     7261 aaacgaatct cgtgtttgct gccatcctcg gcgccgggat ggtaaccgtg ctgagtttgc
     7321 acgttgccgc cgcggtgcgc gaacgccgtg tggactccag tgtggtccga gccaatgaag
     7381 acggcttcgt ttacgtgtgt gatgtggaga gcattccgga gaagagggcg aggattgtct
     7441 cgctcagcgg agaacgggtc gccgtgtttc ggtatgacgg caaggtctcg gcgatttcaa
     7501 atgtgtgcca gcaccagaac ggtccgctcg gcgaaggccg gattatcgac ggttgcgtga
     7561 cctgtccgtg gcacggctat cagtatttgc cagattcggg tgcatctcca cccccattca
     7621 ctgaaaaggt gcccaccttc cgcacgcgtg tggccggtgg caaggtgttt gtccatcctc
     7681 gcccccacgc ccctggaacg cgcctcgagc cggccaggat cgacggtcag acagagaggg
     7741 tcgcggtatg aacaacgatg agttctatgt cgggtacgca ccaaaacccc cggaacacct
     7801 gagacggacc atagtcaaga tcgtaattgc cctgaatgcg attgcagtgg tgctcgcggt
     7861 tctgctcgtg cttggccaac aacatttcgc cgcgagcgcg tttgagttcc agcaattccg
     7921 tgagttcgag ggagttcttc tggagcaccc ctatccgagc ctgctggttc gaaggccggg
     7981 aatggcggag ggaaccttgc cgttttcgcg ttacttgctc gtggcgtcag gcaagcacgc
     8041 ggcgtcttcg gttgtttctg gactggatgg cagaaaggtc cgcattcggg gatcgctgat
     8101 ctatcgtgag ggaaacacaa tgatcgagct ggcttcgggt gctgccccag tcgctgaaga
     8161 agcggattcc aaccgcgcgg ctacggcgtc ggaccttggc caagtgacgc tttcaggcga
     8221 gatcgtggac agcaagtgct acttaggcgt gatgaacccc ggtagcggca aagtgcatcg
     8281 cgattgcgcc gtacggtgta ttagtggcgg gatccctccg gccttctccg tgaaggacgc
     8341 cgagggcagg ccgaagatcc tcttgctcgc cggagtcgat ggccgccagt tgaatcgcga
     8401 ggtgctcgac ttcgtcgcgg agccgatcac aattcgtggc cggctcacgc gatcgggcga
     8461 gacttacatc ctgtggaccg aacccagctc cttcgttcgg cgtcactaat aacaacgaac
     8521 ccaagcgggt attctcaagt cgaggacgaa ttccaatgac agagccgacg attgacgcgg
     8581 ttcttcgaaa cctctttccc gttcccattc ccaaacccgt tgatggcgcg ctcgggcgca
     8641 tcctcggact gcgccaggct gcgaacctct accgcgatct gattgcggag cgagacgatc
     8701 gtccgcttac ggaccggctg ttgagtcatc tccatgtcac gtatcgcgtc tcagaccgtg
     8761 acctggaccg catcccgcgg ctcggaccgg tagtgattgt ggccaatcat ccatacggca
     8821 tcctggacgg agccgttctg gcttcgatcc tgcttcggtt gcgccctgac gtgaagtttc
     8881 ttgcgaatca aatgctttcg gtgattcccg aaatccgcga cctgctgatc ccggtggatc
     8941 cgattggtgg agcggcatct acccaaataa acgggcgcgg ggtcaagcag gcactcgatt
     9001 tcctcagcgg cggtgggttg ctcgtcgtgt tccccgcggg agaagtctcg cacttccgct
     9061 ggaaggagcg ttccgtcgcg gacccggaat ggaatccggc gatttcgcga gtcatcgcca
     9121 tcgcttgccg ccgtgtgcga ggttttagcg tggtgccggc gtacgtgagt gggagtaata
     9181 gcttcctctt tcagacgctt gggatagtgc acccgcgatt gcgcacggcc ttattggtcc
     9241 gcgaactgct gaacaagcgc cggcatgacg tcgaggtccg tatcggccat ccccttctcg
     9301 gtgagagact gcttgcgatt cccaccgatg gtcagagaac tcagcacctg cgttggcggg
     9361 ccaacctgct ggcctgccgc aatccattca aggcgaagac agcttcgtcg ttgcggagtc
     9421 ggaggcgtcg cgaaacacag gagccgattg ccgccgccat gcctgcggag ggcctgacgc
     9481 gagaggtcca agcgcttggc gcgttattaa agtccagcga atttgaagtt tatctcgctc
     9541 cggctgacag gattccggcc gtcctggcgg agatcggacg cctgcgggaa gtggcgtttc
     9601 gggccgccgg tgaaggaacc ggcaaggctt ccgacctgga tgcctttgac gctcattacc
     9661 tccatctgtt cgtctggaac acggacaggc atgaggtcgt aggagcgtat cgcttggggc
     9721 cggtggattc gatccggcgt cgactgggga tcgccgggct ctacacggca actctgttta
     9781 actacggaga cgagttcctt gaccgcctag gtccggcact ggaactgggc cggtccttca
     9841 ttaggcctga ataccagaga gcctttgctc cgctgctctt gctttggaaa ggcatcggta
     9901 agtacatcgc ggagaatctt cggtacaagg tcctgttcgg cccggtcagc atcagcaatc
     9961 aataccagtc ggtgtcccgg cagctcattg tgtctttcct tgagtgccat gctgcgctaa
    10021 acgagtgggc cgcgctagtt tcctcgaaaa acccgttccg ttctcgcgcg gccaggcctt
    10081 ggaaaggcga gggttacacg agccccggac tggacatcga ggacctttcc gcggcggtgg
    10141 ccgatattga gcctgctcag cccggcgttc ccgtcctgtt gcggcaatac ctcaagcttg
    10201 gtgggaagct gctgggcttc accgtggacc cggaatttgc cgactcgctg gacgggctga
    10261 ttatggtgga cctgaccagg accgagttga agcttttgga gcggtatctc ggtaaagtcg
    10321 aggccgccga gtttctcaat tttcactggg gagaatatga tccgcaacaa acgtctcaca
    10381 cttctgagtt ggacgctgcg agtcatcgca gcggtgattc tgctgcaaac gctgttcttc
    10441 aagttcaccg gcgcggctga atcggtgtac atcttcacta cgctcggcgc agagccctgg
    10501 ggacggatcg gcagcggcgt cgtggaactg atcgcgtcca tcctgctgct tgtgccgcgg
    10561 acagtcgtct acggcgccat tctatctctg ggcactatgg gtggagccat cttcagccac
    10621 ctgacgaagc tcggcgttgc gctcccggct gtcgacgatc atggcgagct ttttgcgctg
    10681 gccgttctgg tgtttgtttg ctcggccgca gtgctttttc tgcaccgcca cgaactgccg
    10741 ctcgtgcgcc atcgagcggt gacaccggtg gctggccggc tcggatgagt tgaacgacgt
    10801 gaggccgggt gaatccggcc ctacactcgt tcgatttctt ttgccaggaa gaagttgagt
    10861 gctgtccgca gcacggcaat tgcagccagt tggccgatgt cgttccaggt cggtgcgatt
    10921 gcggtgcgga ggatgtcagc ggcgagtgcg aattccagcc cgaccgccag ccagcggcca
    10981 agagcaagac gaaggtcgac tttggcttcc tgaggtgtgt tgcggcgaac gaagagcggc
    11041 gcagcgcgca ccgcggcttc ggctaccgcc agggtaatta tcgctgccgc ggcgatctcc
    11101 accgcgatag ccaggtattc gatcccaagg atggagtatt cacgcatgaa tcactcgccg
    11161 gttcggcgcc atctgctcgc tgaagccacc ctcccggcgc acgcgtttcc agagcaggcg
    11221 gatgaattcg ttcactgtca tcgggttcgc acccagtctg cggttggtcg cgccaatctc
    11281 gaaagcgata cgccactgcg tcagcatcgt gcgataggca tcaagcaggc tgactgcggg
    11341 atcgtaaata tgggtggctt cagcggtcac accattcaat tcgatgacct tgagcggttg
    11401 gccttccttg aaggcgtcga tcgagggtgc gcgaatgtcg aaccggccaa agaagaagcc
    11461 gggatgactt ttgctgatcc tgtcgatcgc ggcttcgagc ggctgggtaa tcaggcggct
    11521 gccatccaag aagaccgcac cgcggcagtg cgatccgagt tctgccaggc ggaccgcttc
    11581 tcccggggcc ggaacttcgt gagcggaacg cccgatacgg cccaagtacg tagaagccag
    11641 gcagaccgct cggtcgtctt ttaggaccag ttcttcgaga gtgttccggc catttccgat
    11701 gacctccgga aaccgcttct tggtaatcga tagaatgtgt ccggttggct ccaagggata
    11761 ccggtaatag aagacgccga actcgtatcc ctcgatgtat tcctgcagga tcgtgtcgcc
    11821 gttggccgct tccaggtagg attccatttc gccaagggag cggatgatgg caacgccaga
    11881 accgcgttcg ccaacgtccg gcttaaggac gaccggaaac gctagaccgt gctgctggag
    11941 gaatgcgttt gccttccgaa ttcgcgcagt ggactccagc gaacccggga ttctggtata
    12001 cgcagccaca atgcccggta ttcggtccag atgatctagg atctctgact tggattcgcc
    12061 aatgaagccg ccggatggca taccgggatt ggcagctgtg aacagggtcg gcgaacgatg
    12121 cttgagcgcc agcagggcga gatagggtat cagcgggaga taagcagccc aaggcggcca
    12181 gaattcccag cgaacttttc gtttcaggaa accggcgagt ttcctgcggg tgcgaaaccc
    12241 gaacagcagc ggcagctcct ggcgtagccc gatccaaagc acaaagatcg cgacaatcgc
    12301 catcgagctc tttccgccgg cgaatagcat cgttcgaacc accgggccgc cgagggccac
    12361 ggtaaggcaa accaccaatg gcacccagat cgccgctgca agcaggaagt acattgagaa
    12421 tcgccagact ctcgtcctga gcaggccggc agcgaagtag gtcggcagcc gcaaaccggg
    12481 tgtgaatcga ctgagaagca cgaccgcggc gcccttcgag gaaagccatg aagacgcacg
    12541 atcgagttgc tccggcgaga cgaacctccg aaacagcggc cagcggatca cgggtcggcc
    12601 gatgcaccgc ccgcccagga acaaaagaag atccccgaag tagattccag ccagacaggc
    12661 cagaatcccg ctgatgaaac ccactttccc ctgagctacc aagacgccgg tcgcgatgca
    12721 ggtcagatct tcggagacga gggtcgccag agcaatcaga atcaggtgcg gcatgggagc
    12781 gtcactcttt ctccgggcag tcgcctgcac ggagccgccg gcgtataggt caatgtcacc
    12841 gcggattccg tcacccgaac gtgggtgaag ctcacagtct cggcgtccgc gcgatgcata
    12901 cacggagagt aggcggaggg ccgatcgccg tggctcttat ggaatgcgtg gagtacgtcc
    12961 gagtcgagcc ttgccgcgga gccggccgta cgagccaact cctcccttcg cgaggccagc
    13021 acacctgtct gatcgaataa cgacgaaacc aatggcatgt aggggtcgcc gtacggcact
    13081 atggccgtct cttcgccagt ccactcgagg acggctgcgg gcaggccggg ttcaagagca
    13141 gccagcgtga aaggcgtaaa cggagagagg tcctgtcgac agacacgttc gttgatttcg
    13201 gcaagacacg tcgcgcttag cagctctagt accagcaatc cacggctcct gtgtggcgcc
    13261 ggtacgcggc cggccagccg aggagcaccg ttgagcaggc atagcgacac accgaattcg
    13321 ttgaccccga tccatgcgcc accgaaatcg ccgtcgatcg gcgcaacaaa ccggactccg
    13381 gctcgggcgg caatccgggg accgagcgcc ggcgcccggc taatccgctc gtcgcgatta
    13441 cagaagagtt ggtagccctc gtcctcatgg acccagctca ccgtacacac ttcgacgcct
    13501 ccgccagata ccgcaaggtg gacggcgcca tcccgaaatc gtgttccact ggaatgccga
    13561 gcgccttcag aaccagtgcg atcagtgcga agtgatgaac agtgtgactg ctcaggactt
    13621 gcaactcgcg tcccaccgag gatgccagga aggaatcgcc gtcttccatc cggacattca
    13681 ccacccgctc gctctcgaaa tcgccgatca gtagccgcag catgatttcg cgcgtcttcg
    13741 ccagagccgc tctgcagtct gtctcgacgg taatgtctct cttgcgggcg tcgtagtcga
    13801 tccggcccga ccccaccccg tcaagaaaac attcgtagaa ctccaggatg tgcctcaggt
    13861 gcccacccac gcgatgcgga gtaacagccg ggggcgactc cgcatacgct ctggagtcca
    13921 ttccttccag tagcgacttc acctgatgga gcagccggac gctcgtttca gtcaggacag
    13981 tactgctctc cgttagggtc atgtcttctt tgaaatcccg tgacggaact cgcttattcc
    14041 cgagtagggg ggaataccag cccctacgcg aggcattaca catgcgaatg cggacttcga
    14101 cggcaagcgc tcagcagacg gccctgcgcg gcgagtggta tgaagccctg gcggaccact
    14161 ggccggaata tctgatggaa ggtgcggcgc tcggcctttt catgatctcg gcctgcgtat
    14221 ttactgtact cctggagcat ccttcatcat ggctgcatca ggctattccc agcccggtgc
    14281 tgcgccgtct gctgaacgga gtcgcgatgg gctgcaccgc aatcggactc atctattcgc
    14341 catggggcaa gcgttcgggc gcgcatatga acccttcggt aaccctgact tttctgtctc
    14401 tgggcaagat ccagcggtgg gacgcaatct tctatgtaac cgcgcagttc ctcggcggtt
    14461 tgttcggggt tgttctcgcg gcgatgtcaa tcggactgcc gctgcgccac tccgcggtca
    14521 actacgtggt gacagtgccg ggcacgggcg gtccgggcat cgcattttgg gcggagctgc
    14581 tgatctcctt cctgatgatg tggatgatcc tgttcgcttc gaacacgaag cggtttgaaa
    14641 ggttcaccgg cgttttcgcc ggtttccttc tagcggcctt cattaccttc gaggcgccgc
    14701 tgtccggcat gagcatcaat cctgcgcgga cactcggttc ggcgatcccc gcaaatgagt
    14761 ggaccgcgct atgggtgtac ttcgcgggac cgccgctggg gatgttcctg gcgagcgcgg
    14821 cttaccggct tcgctgggga gcccacgccg tcttctgcgc caagctgcat caccacaacg
    14881 ataaacggtg tattttccgc tgcaattatg cggcaatgtg aaacgcgatc gctcggggag
    14941 gtgtgtgtca atgtcgtcca gtaaccacta cgacgtaatc gtcattggaa ccggagccgg
    15001 cggcggaacg cttgcgtaca aactggcgcc agccggaaag cgaatcctgc tgctggaacg
    15061 cggcgattac gttccgcgcg agaaagacaa ctggaatccc cgcgttgtga acgtcgaagg
    15121 caagtaccag accaaggaga tctggaagga tagcgacgga aaggatctgc atccccacac
    15181 caactattgg gtgggcggca acacgaagtt ttacggtgcg gcgttattcc gtcttcgcaa
    15241 ggaggatttc ggcgaacttc gccatcacgg cggagtatcg ccggcctggc cgatttccta
    15301 cgacgagctg gaaccttact acacggaggc ggagcgactg taccacgtgc acggccagcg
    15361 cggcgaagat ccgaccgagc cgcccgcccg cacgccctac ccgcatccgg cggtcagcca
    15421 tgaacctcgg atcgagcagc ttagcgagga tttcgctcgt aacggagcaa cgccgttcca
    15481 cactccgctc ggtgtgatgc tcgacgaagc gtcccggcac acaagtcctt gtattcgctg
    15541 caacacctgc gacggcttcc cttgtctggt gcacgcgaag tccgatgcac aagtcgtcgc
    15601 cgtggacccg gcgcttgcac acccgaatgt cacgttgctg aggaactcct tcgtcgagcg
    15661 gctggaaaca agtccgtccg gccgcgaagt gacaagcgtg atcgtcacgc gaaatggcgc
    15721 ccgtgaggcg ttctcggcgg acatcgtggc cgtctcctgc ggcgccatca actccgcggc
    15781 tctcctgttg cgctctgcca gcgacaagca cccaggcgga ttggcgaacg gctccggcgt
    15841 ggtgggacgc cattacatgg gacacgtgaa ctcggtcttg atggcgattt cgaagtgtcc
    15901 taatccgact gtcttccaga aaacgctctc ggtaaacgac ttctacttcg gctccaaaga
    15961 ctgggaatac cccatgggcc atatttcgtt cgtcggcaag ctggacggcg agaccctcaa
    16021 ggcgggcgcg ccggcgatcg cccccggttt cacgctcgac ctgatggcta gccactcact
    16081 cgatttctgg ctcacgtccg aagaccttcc cgatccgaac aaccgcgtga cgctggaccg
    16141 cgaggggaac atcgttcttg cctacaagcc gaacaacgaa gaaggccaca aacggctgat
    16201 cgcaaaactc cgcgatctga tgaaccagca acgcaaatgc cccatgcacg ggcatgaatg
    16261 ccatcaggga ctgttcgcgc ggagtctcta tctcggacag cgcattcctc tggccggcgt
    16321 ggcgcatcag aacggcacaa ttcgattcgg ccgcgacccg aagacttcag cattggacgc
    16381 gaactgcaag gcgcacgacc tggacaacct gtacatcgtc gatgccagtt tcttcccgtc
    16441 ctcgggcgcg gtcaacccgg gactgacaat catggccaat gccttgcgcg tgggagcgca
    16501 cctcatagag cggatgggct aggacatgca tcggcgagcc tttctcattc tcgttctgct
    16561 tgcgtcgacg ctttgcgcca acgacgtgtc tccgctggtc acggcggtgg actcgatcgg
    16621 tctcacggtc tccgatctcg accgctcagt ggagttctat gccaaggtac tctctttcga
    16681 aaaagtttcc gaaggagaag tcgccggcga ggacttcgag cacctgcacg gtgtctttgg
    16741 actgcggttg cgtaccgcgc ggatgcgtct cggagacgag ttcatcgaac tgactgagta
    16801 cctggcgcca agaggccgtc ctgcgcccgc cgacgctcgc agcaatgacc gctggttcca
    16861 gtacattgcc atcatcgtca gcgacatgca caaggcctat cagtggctgc gccagaacaa
    16921 agtccagcac tcctcgccgg gaccccagcg cctgcccgat tggaacagga acgccggagg
    16981 catcgaagcc ttctactttc gtgatcctga cgggcatcca ctggaaattc tggcattccc
    17041 gccggacaag ggcgacccga agtggcgccg ctcggcggac aagctgttcc tcggcatcga
    17101 tcacaccgcc atcgttgt
//
LOCUS       DATIFQ010000123         8794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1956364, whole genome shotgun sequence.
ACCESSION   DATIFQ010000123 DATIFQ010000000
VERSION     DATIFQ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956364"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VLE22_05505"
     CDS             complement(<1..713)
                     /locus_tag="VLE22_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB13893.1"
                     /translation="MTGTHSTRRVVRFGVFEADLATMELRKSSLKLRISEQPFLILAA
                     LVERPGEVVGREELRQRIWPSDTFVDFDHSINAAVNKLREVLGDSAENPRYIETFPRR
                     GYRFVYPIETQCNGEPEVAAAEAVTTQAAALRRRSWALFYVGVTLVLALAAAFLALRH
                     YLTTARKPDTLLRPVPLTTYAGSEYMPSFSPDGNQVAFAWTGPNGDNLDVYVKMIGTE
                     NAVRLTTGPGPKAAPEWSP"
     gene            complement(790..1617)
                     /locus_tag="VLE22_05510"
     CDS             complement(790..1617)
                     /locus_tag="VLE22_05510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB13894.1"
                     /translation="MFFSDIVLARRLEGGEAESARECAEAMVRLWPASDGAVMAVGSG
                     WAAFAGATSPLSHAVGLGMNEPMTVQEIERVEEFYRSRGAAATIDLCPLADPSLCGIL
                     AARGYHVAEFNNILVRRLDEGEPSPETECGVEVRETAESDATVWTDTLARGFFEGNEP
                     GTEELEVGQILFRMRSGVCYLARVDGLPSGGGALAVRDRLAMLYADATHPFFRRRGVQ
                     VALIAARLRSAVERGCDLATAGTLPGTVSQRNYERLGFRVAYTRAVMVREWQQSHAG"
     gene            1685..2491
                     /locus_tag="VLE22_05515"
     CDS             1685..2491
                     /locus_tag="VLE22_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13895.1"
                     /translation="MQPFHIIVCGSIVPDPLQTLEPVTGPSGPALKNEMMLPAVLDPW
                     AAHALFEAANLAKKAPGSKVWLVSLGPKAKLQQVMMTVAQKVPFEFVGLDGPAGGFTE
                     AYNVATVLAEAIQAIPGLDHSRLLIFGGWESASRGAGVTMQMVGERLGITDQFQGVDE
                     LSVAADGSLQVLERIEGGRHQVSVCDGPPALLGWATGNLPEPPNNPQIGMTNMRAIMP
                     ALQRAKQVKLTGEGLSFSAVSLPSQRRETKIVKDLPADEIAREIVEWIRS"
     gene            2498..3529
                     /locus_tag="VLE22_05520"
     CDS             2498..3529
                     /locus_tag="VLE22_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13896.1"
                     /translation="METILFLAHTENDGTLARPALEALAGAKWLAGALPGSTFAAGLI
                     GERTQPAAGQTASCGASRFLSVTGSEFGQPRYATDAAAAEALCRAAGTTIVIAPATTR
                     WARSLAGVAQRLDGRIDSHVTGISAVGGKLVVSRWYYRQRMEAVLDRTKRPWLILLET
                     SVFPAWSGEPGSAPVEAVAVDVPDSAKRTRFVGVRAPKTDEQTIRPDANLLFVTGAGW
                     TKKQSDGQVHLADAAELILGFLRKTQSSLGSSKSLVDLSGEGQVVLPFLTHLNQVGQT
                     GSTPRHPKGLATCCHGEEPHVVGWRFINERRAVNLDPNCGWARGKADVLYVADAFEVM
                     KKVNALLAD"
     gene            complement(3556..4875)
                     /locus_tag="VLE22_05525"
     CDS             complement(3556..4875)
                     /locus_tag="VLE22_05525"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB13897.1"
                     /translation="MLPGFARHNSTVYRQYVENRRTFLKTAGLVSAHAAAGRVLGANE
                     KIVVALIGCRNQGRGDALGAIKQGAEIKTICDIDDEIIAKVSPQIEKAQDKAPTVVKD
                     YRRVLEDKDIDGVIIATPDHWHAIQTIHACQAGKDIYIEKPLSHTIQEGQMMRDAARK
                     YNRVVQVGTQRRSGEHFRTAIEYVGSGKLGKVCMVKAWINQLRKSIGNPPDGTPPPGA
                     DYDVWLGPAPKRPFNPNRFHYNWRFFYDYCNTELGNQGVHMLDVGMWGIQKLRGVENS
                     LPKRISSNGGIYWLNDAKEVPDTQVVTYDYGDMMLVWEMHSFQSMHPIEGDYAGTAFY
                     GTEGTLVVEDGWKVYGPKNEVITSAEPSGGSHPGNWLQCIKSRQWPNADIELGRLSTT
                     ICHLGNISHRLGRDVKFDPKTETFPGDKEANAWLTKQYRAPYTLPKV"
     gene            complement(4923..5660)
                     /locus_tag="VLE22_05530"
     CDS             complement(4923..5660)
                     /locus_tag="VLE22_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB13898.1"
                     /translation="MNPAVEELTERGEQLRRDGVVIRTYDLWKTYLMGDQEIHAVSGV
                     DVEIRRGEYVAIMGPSGSGKSTLMNLIGCLDTPTAGQYYINGRLVSEMNDDELAQIRN
                     KEIGFVFQTFNLLPRATALHNVELPLIYAGIAADQRLERAKQAMRQVDLEQRMYHKPS
                     ELSGGQRQRVAVARALVNNPSILLADEPTGNLDTATGNEIMALFERLHQQGNTIVLVT
                     HEHDIAMHAHRVVHVRDGKVEKDEKLK"
     gene            complement(5692..7107)
                     /locus_tag="VLE22_05535"
     CDS             complement(5692..7107)
                     /locus_tag="VLE22_05535"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB13899.1"
                     /translation="MKKKWKIWLAILGVLVIGASVYVGIRKSRAGIVTVQTGRVARQD
                     LTSLVTASGEIKPRNYINIGANAMGRITEILVREGDRVRKGQLLAKLESVQAESEVQA
                     QKAMLSSSEADAAASEAAVRSADENLRTAQASIDRAKAELERASLNFKRAEKLYNEKL
                     IARQEFDQRKAEYESMDAAVREAEARLAQARAQRGQATSQLTATQRRVGQARANVRRV
                     TDVLEKHLAVSPLDGVVTNLPVRVGETVVPGIQNSSASLIMTIADMSLITAEVKVDET
                     DIVNVQLDQVADITIDAIPGKTFKGHVIEIGNTAILRSTGLAASQSAISSQEAKDFKV
                     VIALDSPPAEIRPGLSCTSKITTATRQGALTVPIQALTIRQKGDLEPPPKKGVQAAAK
                     VDPAKEKERKEEVQGVFVVSGDKVTFRKVETGITGSTDIEVLSGVKAGEEIVTGSYKV
                     IRTIRSDTRVKVDNKTPAKTE"
     gene            complement(7216..7476)
                     /locus_tag="VLE22_05540"
     CDS             complement(7216..7476)
                     /locus_tag="VLE22_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13900.1"
                     /translation="MTAIVVTAFVLAVIGLLVYSSLGLRKHRAEVCVSFQGRTNCRVA
                     SGATREQAVRTATDNACALIASGMTESMACTSAPPVSVKWLD"
     gene            complement(7479..8324)
                     /locus_tag="VLE22_05545"
     CDS             complement(7479..8324)
                     /locus_tag="VLE22_05545"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HSB13901.1"
                     /translation="MDTSQPAASQAAVSPSWERVQLARHPKRPHSLDYIQRLITDFSE
                     LHGDRAFGDDAAIVCGFGFFEGIPVVVAGQQKGRDTKQKLHRNFGMPKPEGYRKAMRV
                     MEMAAKFGRPVITLLDTPGAYPGIDAEERGQAEAIAMNLREMSRLGVPIIAICIGEGG
                     SGGALALGVGNRVYMLENAIYSVISPESCAAIIWRDSGKAELAATALKLTAEDLLRFG
                     LIDGIIPEPEEGAHSDHDKAVETVRQTLRQALEELRQLSPEALVEQRYQRFRKMGNFF
                     SEAGG"
     gene            complement(8440..>8794)
                     /locus_tag="VLE22_05550"
     CDS             complement(8440..>8794)
                     /locus_tag="VLE22_05550"
                     /inference="COORDINATES: protein motif:HMM:NF022664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HSB13902.1"
                     /translation="SGTVRMVRQISGSPCRQNETWGYDSNGVWVDRGCRAEFEIVPRN
                     DTWGWGTGSSSVKTITCSSNSGNRVHCNADTSGGVRMLRQVSGSPCRQNETWGYDSTG
                     VWVDRGCRAEFEVRK"
BASE COUNT         1476 a         3091 c         2457 g         1770 t
ORIGIN      
        1 tccggcgacc actccggcgc cgctttagga cccgggccgg tcgtcagccg caccgcgttc
       61 tccgtgccga tcatcttcac gtagacgtcg aggttgtcac cattgggccc cgtccaggcg
      121 aaagccactt ggttgccgtc cggagagaag ctcggcatgt actcggagcc cgcatacgtg
      181 gtcaggggaa ccggcctcag cagcgtgtcg ggctttctcg ctgtcgtcag atagtgccgc
      241 agggcgagga acgcagccgc cagcgccaac accagggtca ccccgacata gaacagcgcc
      301 cacgaccgcc ggcgcagagc cgctgcctgg gtcgttactg cctccgccgc tgcaacctcc
      361 ggttcgccat tgcactgcgt ttctatcggg tatacaaacc ggtatccacg ccgtggaaac
      421 gtttcgatgt agcgcgggtt ctcggccgaa tcccccagca cctctcgcag cttgtttacc
      481 gcggcgttga tgctgtgatc gaaatcgacg aacgtgtccg aaggccagat ccgctgccgt
      541 aactcctccc gcccgacaac ctctccggga cgctcaacca gcgccgccag aatcaaaaaa
      601 ggctgttcgg aaatgcgcag cttgaggctg ctcttacgaa gctccatcgt cgcgagatcc
      661 gcctcaaaga ctccgaagcg cacgactcgt cttgtcgaat gtgtgcctgt catcacgctc
      721 gccttcacac gcaaactata tcgacgctaa tttccttcga cctgttcccg tgaaggattg
      781 accctgccgt cacccggcgt ggctctgctg ccactcccgc accatcaccg ctcgcgtata
      841 cgccacccgg aacccaagtc gctcgtagtt tcgctgagac acggtgcccg gaagcgttcc
      901 cgccgtcgcc aggtcgcacc cccgctccac tgccgaccgc aaccgcgccg cgatcagggc
      961 cacctgcact ccccgccgcc ggaagaacgg gtgtgtcgcg tcggcataca gcatcgccaa
     1021 cctgtcgcgg accgccagcg ccccgcctcc ggagggcagt ccgtctacgc gagccagata
     1081 acagacgcct gaccgcatac ggaacagaat ctgtccgacc tccagttcct cggttccagg
     1141 ctcgttcccc tcgaagaatc cgcgggcgag tgtatccgtc cacaccgtag cgtccgattc
     1201 ggccgtctcg cgcacctcca ccccgcactc cgtctccggg ctcggctcgc cttcatcaag
     1261 acggcgcacc aggatattgt tgaactccgc aacgtggtac ccgcgcgccg caaggattcc
     1321 gcaaagcgac gggtccgcaa gcgggcacag gtcgatggtc gccgccgcgc cccggctgcg
     1381 gtaaaactcc tcgacgcgtt cgatctcctg caccgtcatc ggctcgttca tcccgagtcc
     1441 gaccgcgtgg ctcagcggtg acgtcgcccc ggcgaacgca gcccacccgc tccctactgc
     1501 catcaccgct ccgtccgagg ctggccacag ccggaccatc gcctcggcgc actccctcgc
     1561 actctcggcc tcgccaccct ccaggcgccg ggcgagtacg atgtccgaga agaacacggc
     1621 ttcagtgtat ccgaatctct tccagacagc gcggccgcgc gtcgcgcctg catatactga
     1681 gtaaatgcaa ccgtttcaca tcatcgtctg cgggagcatt gtcccggacc cgctccagac
     1741 cctggagccg gtcaccgggc cgtccggtcc cgccctcaag aacgaaatga tgctccccgc
     1801 cgtgctcgac ccctgggcag cgcacgccct tttcgaagcc gccaacctcg cgaagaaagc
     1861 acccggcagc aaagtttggc tcgtcagcct cgggcccaag gcgaagctcc agcaggtgat
     1921 gatgacggtc gcacagaagg tcccgttcga gttcgtaggg cttgacggtc ctgccggcgg
     1981 tttcaccgaa gcatacaacg tggcgactgt acttgccgaa gctatccagg ccatccccgg
     2041 cctcgaccat tcgcgccttc ttatcttcgg tggttgggag tccgcgtctc ggggtgctgg
     2101 cgtcaccatg cagatggtcg gcgagcgcct cggcatcacc gaccagttcc agggcgtcga
     2161 cgagctctcc gtcgccgcgg acggctccct ccaggttctc gaacgcatcg agggcggccg
     2221 ccatcaggtc tcggtctgcg acggcccacc ggcgcttctc ggctgggcca ccggcaacct
     2281 gcccgagcct cccaacaatc cgcagattgg catgaccaac atgcgtgcca tcatgcccgc
     2341 gctccagcgc gcgaaacagg tgaaactaac aggagagggc ctcagcttca gcgccgtcag
     2401 ccttcccagc cagcgtcgtg aaacgaaaat tgtgaaggat ctgcccgccg acgagatcgc
     2461 gcgggagatc gtcgagtgga tcaggagcta gaccgccgtg gaaaccatcc tctttctcgc
     2521 acacaccgaa aacgacggca cgctcgcccg cccggcgctc gaggcgctcg ccggcgcgaa
     2581 atggctcgcc ggcgcactgc ccggctccac cttcgccgct ggattgatcg gtgagagaac
     2641 gcaacccgcc gccggtcaga ctgcgtcgtg cggcgcctcc cgcttcctct ccgtcacggg
     2701 atccgagttc ggccagcccc gctatgcgac cgatgccgcc gccgcggaag cgctgtgccg
     2761 tgccgctggc accacgatcg tgatcgcccc cgccaccacg cgctgggcgc gttcactggc
     2821 gggcgtcgcc cagcggcttg acggccgcat cgattcgcac gtcaccggaa tctccgcggt
     2881 gggcggcaaa ctggtggttt cccgctggta ctaccggcag cggatggaag cagtcctcga
     2941 tcggacgaaa agaccatggc tgatcctgct tgagaccagc gtcttcccag cctggagcgg
     3001 cgagccaggc tcagccccgg ttgaggccgt cgccgtcgat gttcccgatt ctgcgaaacg
     3061 aacccgcttt gtcggggtcc gtgctccgaa aaccgacgaa caaacaatcc gccccgacgc
     3121 gaatctgctc ttcgtcaccg gagccgggtg gaccaagaaa caatccgatg gtcaggtgca
     3181 cctcgccgat gccgcggaac tgatccttgg tttcctccgg aaaacacagt cgtcgctcgg
     3241 cagcagcaaa tctctggtgg acctctcggg cgaaggacag gtggtgctcc ccttcctgac
     3301 gcatctcaac caggtgggcc agactggttc cacgccgcgc cacccgaagg gcttggccac
     3361 gtgctgtcac ggtgaggagc ctcacgtcgt cggctggcgc ttcatcaatg agcgcagggc
     3421 cgtcaacctc gaccccaact gcggctgggc gcgcggcaaa gccgatgtcc tctacgtcgc
     3481 cgacgccttc gaggtgatga aaaaggtgaa cgctctactg gcggactaac ggctttccgc
     3541 gccctctgcg gtgaactaca ccttcggcag tgtatacggc gcccggtact gcttcgtcag
     3601 ccatgcgttt gcctctttgt cgcctgggaa agtctcggtc ttcggatcga acttcacgtc
     3661 tcgccccagc cggtgagaga tgttgcccag gtggcagatg gtcgtgctca gccgcccaag
     3721 ctcgatgtcg gcgttcggcc actgccgact cttgatgcac tgcagccagt tgccaggatg
     3781 cgatccgccc gacggctccg cgctcgtgat cacctcgttc ttcggaccgt agaccttcca
     3841 accgtcctcg acgaccagcg tcccctcggt accgtaaaac gccgtccccg cgtagtcgcc
     3901 ttcgatcgga tgcatgctct gaaaactatg catctcccag accagcatca tgtcgccgta
     3961 atcgtatgtg accacctgtg tgtccggtac ctctttggcg tcgttaagcc agtagatgcc
     4021 accgttgctc gagatccgct tgggcaacga attctcgacg ccgcgcagct tctgaatgcc
     4081 ccacatgccg acatccagca tgtgtacgcc ctggtttccc agttccgtgt tgcagtagtc
     4141 gtagaagaac cgccagttat agtgaaaccg gttcggattg aacggacgtt tcggcgccgg
     4201 cccgagccac acgtcatagt ccgcgcccgg cggcggcgtg ccgtccggcg ggttgccaat
     4261 gctcttgcga agctggttga tccacgcctt caccatgcac accttgccga gtttgccgga
     4321 gcccacgtac tcgatcgcgg tccggaaatg ctcgccgctc cgccgctgcg tgcccacctg
     4381 caccacgcgg ttgtactttc gcgccgcgtc gcgcatcatc tgcccttcct gaatcgtgtg
     4441 cgaaagcggc ttctcgatgt aaatgtcctt gcccgcttgg caggcgtgaa tggtctggat
     4501 ggcatgccag tggtccggcg tggcgataat gacgccgtcg atgtccttgt cttcgagcac
     4561 ccgccggtag tccttcacca ccgtcggagc cttgtcctgc gccttctcga tctgtggcga
     4621 caccttcgcg atgatctcgt catcgatgtc gcagattgtc ttgatctccg cgccctgctt
     4681 gatcgcgcct agtgcgtcgc cgcgcccctg gttacggcac ccgatcagcg cgacaacgat
     4741 cttctcgttc gctcccagca cgcgcccggc cgcggcatgc gcggacacca ggccagccgt
     4801 cttcaggaat gttctccggt tctccacgta ttgacgatac accgtggagt tatggcgggc
     4861 aaaccctggt aacacccgtc ctctttggtg tcttcgtgcc tttgcggtga accggccccg
     4921 ccctacttca gcttctcgtc cttttcgact tttccgtcac ggacgtgaac aacgcggtgg
     4981 gcgtgcatgg cgatgtcgtg ctcgtgcgtc accagcacga tcgtgttccc ctgctggtgc
     5041 agccgctcga acagcgccat gatttcgttg ccggtcgcgg tgtcgaggtt cccggtcggc
     5101 tcgtcggcca gcagaatcga tggattattg accagcgcgc gcgccacggc gacacgctgc
     5161 cgctggccgc ccgacagttc gctcggcttg tgatacatgc gttgctcgag gtccacttgc
     5221 cgcatcgcct gcttcgcccg ttcgagcctc tggtctgcag cgatgccggc atagatcaac
     5281 ggcagttcca cgttgtgcaa agccgtcgcg cgcggcagga ggttgaacgt ctggaacacg
     5341 aagccgatct ccttgtttcg gatctgcgcg agttcgtcgt cgttcatctc gctcaccaac
     5401 cgcccgttga tgtaatactg gcccgccgtc ggcgtgtcca ggcacccgat caggttcatc
     5461 agcgtggact tgcccgaacc cgaaggaccc atgatcgcga cgtactcgcc ccggcgaatc
     5521 tccacatcca ccccggagac cgcatggatc tcctggtcgc ccatgaggta ggtcttccat
     5581 aggtcataag tgcggatgac aacgccgtcg cgccgtaatt gttcgccgcg ttcggttaat
     5641 tcctcgactg ctgggttcat gctcggtcct cgcttcgaaa aaaatcgcgg gttactccgt
     5701 ctttgccggt gtcttgttgt cgactttcac cctggtgtcg ctgcgaatcg tccggatcac
     5761 cttgtagctg cccgtgacga tctcctcgcc cgctttcacg ccgctcagca cttcgatgtc
     5821 ggttgagccg gtaattcccg tttccacctt gcggaatgtg accttgtcgc ctgaaactac
     5881 gaatacaccc tggacttctt ccttccgctc cttctccttc gccggatcca ccttcgccgc
     5941 ggcctgaaca cccttcttcg gtggtggctc gaggtcgcct ttctgccgga tcgtcagcgc
     6001 ttgaatggga acggtcagtg cgccctgccg cgtcgcggtc gtaatcttcg atgtgcacga
     6061 taagcccggc cggatctccg cgggcgggct gtccagtgcg atcacaacct tgaaatcctt
     6121 cgcctcttgg cttgaaatcg cgctttgcga cgccgccagt cccgtcgagc gcaggatcgc
     6181 cgtattcccg atctcgatca cgtgcccctt gaacgtcttc ccgggaatcg cgtcgatcgt
     6241 aatatcagcc acctggtcga gttgtacgtt gacgatgtcc gtctcgtcca ccttcacctc
     6301 ggccgtgatc agcgacatgt cggcgatcgt catgatcaga ctcgccgacg aattctggat
     6361 gcccggtact accgtctcgc ccacgcgaac aggcaggttc gtcaccaccc catcgagcgg
     6421 cgaaaccgcg aggtgcttct ccagaacatc cgtaacccgc ctcacgttag cacgcgcttg
     6481 gccgaccctc cgctgcgtcg ccgtcagttg cgatgtcgcc tgccctcgct gcgccctggc
     6541 ctgcgccagc cgcgcctcag cctcgcggac cgcggcgtcc atcgattcgt actcggcctt
     6601 cctctggtcg aactcctggc gggcgatgag cttctcgttg tacaacttct ccgcccgctt
     6661 gaagttcaga ctcgcgcgct cgagttcggc tttcgcccgg tcgatcgaag cctgcgccgt
     6721 ccgcagattc tcgtcagccg accggaccgc cgcttccgac gcagccgcat ccgcctccga
     6781 tgagctcaac atcgccttct gcgcctgcac ctccgactcg gcctgcacgg actccagctt
     6841 cgccagaagc tgcccctttc gaaccctgtc tccttctctg accaggatct ccgtgatcct
     6901 gcccatcgcg tttgcgccga tattgatata attccgcggc ttaatttcac cggatgccgt
     6961 caccagggat gtgagatcct gccgggccac ccggcccgtt tgtaccgtga cgatgccggc
     7021 ccggctcttc cggataccga cgtacaccga ggcgccgatc accagtaccc cgaggatcgc
     7081 cagccagatc ttccactttt ttttcacact aagaactccc tgtttcttag acgacgttcc
     7141 agggtccttt gttcataaat cccatcctac cacgcaggcc catctgaggc gtggggaaga
     7201 tgcagataaa ccactctaat ctaaccactt aacagagaca ggcggggcgc tggtacaggc
     7261 catcgattcg gtcatgccgg aggcgatcaa cgcacaggcg ttatcggtag ctgtgcgcac
     7321 cgcctgctcc cgcgtggcgc cggatgctac gcggcagttc gtccggcctt ggaagctgac
     7381 gcatacttcc gccctgtgtt tccgcaaccc gaggctcgag tacacgagca gcccgatcac
     7441 ggccagcaca aaggcggtca ccacaatggc ggtcatcttc acccgccggc ctccgaaaag
     7501 aagtttccca tcttgcggaa acgttggtag cgctgttcta ccagcgcttc cgggctgagc
     7561 tgcctgagtt cctcaagcgc ctgccggagc gtctggcgga cggtttccac cgccttgtcg
     7621 tggtccgagt gagcgccctc ttccggctct ggaatgattc cgtcaatcag cccgaatcgc
     7681 agcaggtcct cggccgtcag cttgagcgcc gtcgccgcca gttccgcctt tccggagtcc
     7741 cgccatatga tcgccgcgca gctttccggc gaaatcacgc tgtagatcgc gttttcgagc
     7801 atatagacgc ggttgcctac acccaacgcc agcgcgccgc cgctcccgcc ttcgccgatg
     7861 cagattgcga tgatcgggac acccagccgg gacatctccc ggaggttcat ggcgatcgcc
     7921 tcggcctgcc cgcgttcttc ggcgtcgatt cccggatagg cacccggcgt gtcgagcagc
     7981 gtgatcaccg gccgcccgaa cttcgcggcc atctccatta cccgcatcgc cttccggtag
     8041 ccctctggct tgggcatgcc gaagttccgg tgaagcttct gctttgtgtc tctccccttc
     8101 tgctgtcccg cgaccaccac ggggattccc tcgaaaaaac caaacccgca aacgatcgcg
     8161 gcgtcatcgc cgaacgcgcg gtcgccgtga agctcgctga agtcggtaat cagccgctgg
     8221 atgtaatcga gcgagtgcgg acgcttcgga tgccgcgcca attgtacccg ctcccacgaa
     8281 gggcttactg cggcctgcga cgccgccggc tgtgacgtgt ccatgaaggt cttttcctac
     8341 actaccactt gaggttgtga atcggctgtc tgtcgctgaa gccgcatccg cacagagccg
     8401 cgacggaacg gagcggtcag aatgcgctct tccgaacggt cactttcgca cttcgaactc
     8461 ggcccggcac ccgcggtcca cccaaacgcc ggtcgagtcg taaccccacg tttcgttctg
     8521 ccggcatggt gagccgctga cctgtcgcag cattctcact cccccgcttg tatcagcgtt
     8581 gcagtgtacg cggttgccgc tgtttgacga gcaggtgatt gtcttgacgg atgacgatcc
     8641 ggtgccccag ccccacgtgt cgttgcgagg gacaatctcg aactcggccc ggcacccgcg
     8701 atccacccag acgccgttcg agtcgtagcc ccacgtttcg ttctgccggc atggcgaccc
     8761 gctgatctgc cgcaccatcc gtacggtccc gctc
//
LOCUS       DATIFQ010000124         6224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_40192, whole genome shotgun sequence.
ACCESSION   DATIFQ010000124 DATIFQ010000000
VERSION     DATIFQ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40192"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VLE22_05555"
     CDS             <1..1180
                     /locus_tag="VLE22_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB13903.1"
                     /translation="DSLTDPLGAVAGASTLEGLIEEVRTHKREVSRTIQWPDGPETFQ
                     AIPLTGREGDLLGVLLAGSSRRELAALIGRIRMAGLAVGGAGLLLGIALSYWVSARIT
                     RPVDQLAAGAREVAAGNWDARVDVRSRDEIGELAEAFNRMTGQLIEQRDRLVQTERVA
                     AWRELARRLAHELKNPLFPLQITVENLQRARAQAPDQFDEIFRESTATLLAELSNLKT
                     IIGRFSEFAKMPPPQFEIVDLNELVQENLRLFDAQFRGEARPRIHTEAHLDESLAKIS
                     ADPGQVLRALQNLTLNAIDAMPAGGTLRFRTRRVEGGVTLEVSDTGEGLTKEECERLF
                     TPYYTTKQHGTGLGLAIVQSVVSDHNGRITVESEQGRGTTFRIELPNAPENDLAPEPS
                     "
     gene            1182..2537
                     /locus_tag="VLE22_05560"
     CDS             1182..2537
                     /locus_tag="VLE22_05560"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB13904.1"
                     /translation="MSHLLIVDDDPNTLASLARAFRLAGHEATVCDNAGRALELIRAQ
                     RFDLVLSDVVMPGKDGLALLEEIKSIDGAIPVVMISGQATVEMAVRATRLGAVDFLEK
                     PISTDKLLLTVENTLKLTRLEEENRQLRRRVGKQEIVWGSEAMRSVMAQIERVAASET
                     RVCILGETGTGKELAARTIHDRSPRKNGPFVTLNCAAVPSELIESELFGHEKGAFTGA
                     ASRHAGRFEQAHRGTLFLDEIGDMPQVMQAKLLRVLEGGEVERVGGDRAIPVDVRVVV
                     ATHRNLSELTAQGKFREDLYHRIYVFPLSLPPLRQRPEDIPLLAEHFSRHVAEINGWK
                     PRPFTAEALEELKRYPWPGNVRELRNVVERLMLLADEVDAETVRLALSGRAPAALASG
                     SGSLAERVDAFEREALLAELRRHEFRMAETARALGLERSHLYKKCQQHGIDLQTLKKV
                     E"
     gene            2592..5813
                     /locus_tag="VLE22_05565"
     CDS             2592..5813
                     /locus_tag="VLE22_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB13905.1"
                     /translation="MSGVWVLVLCCVLTVVAPAQTTHALISGRVVDVQTGLGIGGAQV
                     TYSAAATNASATAHSDDSGYYVLPLLAPGFCRVRVAADGYQPQELYALELPVAGRLDL
                     DFRLRLLADVWQAGEHRSVFFPNSEAVLTFFGPDVDTSRYGSFDANRGRKGSLEPTVS
                     HVIDPFQVSYLPLAGRDVYTMLLTLPGVTADTTTSRGLGLSVNGQRPSASNFLLDGLE
                     NNNYLITGPLTAIAPEAIQEYRVSTNNFSAEYGRTSGFLANAVTRAGGTQWHGLAYFY
                     LKNDVLNANGFQDNRNGQPRRPLKENYMGFQAGGPVPSLPLFTSTAFERLRSRSFGEP
                     TDWLLPSTRFSLFTTPDSVAARLLDRFSPPPATDGNLPYATLRLVPPLSLDRTLVLQR
                     FDYQPEGSRHRLMARLAAATLSRPDFIWTPYHDFVSGLSQDSVSLAFTLTSYLSPRWA
                     SETRAGLNWDDLHWDRPHPEIPTLASRDGAVLPGSPAFYAYRNRTRGAEFVENLTWSR
                     GRHVFKTGFGVLLRGLDGYLTAGRDGLYEFGDIIEFALDSPGFFSASVARASLPDLQR
                     PEFPREYEYRQYYLFAQDAFRPTRRLSLSYGLRYEFFGAPRNTGSVKDAIVNLGTGAT
                     FAERIGSAGLTFDGSGAQRLYDTDANNWAGRFGFSYDLSGNARTVLRGSYGIFYDRPF
                     DNLWQNVQSNSLTLASFSLPLSSRDYLKPIPEALAEFRGRAFSADSPALTLVQPGFRD
                     AYVHSYFLGIQQQVTEDWAVEVNGLGSLGRKLITTDWVNRSFSFPPSTSNPDGRLNPA
                     LPPIFYRANQGSSNYHAMSALVRYRARWSQLQLAYTWSHAIDNQSEPLARLFFDLSAT
                     RATAGSGRGIISAFARQFDSGVDRSNSDFDQRHNLVFFSTWELPGYGRLLRGWRFSQM
                     VAFRSGFPYTVLAPSRFSPGGDLIINNRADIVGPAETTRLPVDGGQLLLDRSLFREPP
                     AGRLGNTGRNALRGPGLFNIDVSLSRSTALPWLGESGRLTLRADVFNVLNHANLGNPA
                     SFLTSDDFGVALYGRRGRESTFPALTPLAELARQVQLILRVEF"
     gene            5813..>6224
                     /locus_tag="VLE22_05570"
     CDS             5813..>6224
                     /locus_tag="VLE22_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13906.1"
                     /translation="MRRLLLAAVLSVGVTQAQPAGVILSITGGLLRRAGTQLALTAKS
                     GDILFPGDALATQNTPAAILFCPEKSLLTLQAQTEVLVDARQLKIRTGSLGTPKPVSS
                     CFLPPVDRTTLASQQHYGASLVRALKPEPPPPQLP"
BASE COUNT         1114 a         1985 c         1954 g         1171 t
ORIGIN      
        1 ggacagcctt accgatccgc tgggggcggt tgcgggcgcg tcaacccttg aggggttgat
       61 cgaggaggta cgcacgcaca aacgggaggt cagccggacg attcaatggc cggacgggcc
      121 ggagacgttt caagcgatcc cactgacggg ccgggagggc gacctgctcg gggtactgct
      181 ggcgggcagc tcgcggcggg aactggcggc gctcatcggg cgtattcgaa tggccggact
      241 ggccgtaggt ggtgcgggac tcctgcttgg gattgcgctt agttactggg tgtcggcgag
      301 aatcacccgg ccagtggacc agctcgcagc cggggctcgg gaggtggccg ctggaaactg
      361 ggatgcccgg gtggacgtgc gctcgcgcga cgagatcggc gagctggcgg aggcgttcaa
      421 ccggatgacc gggcaactca tcgaacagcg cgaccgcctt gtgcagaccg agcgcgtcgc
      481 cgcctggcgc gagctggcgc gacggctcgc ccacgagctg aaaaacccac tcttcccatt
      541 gcagatcacg gtggagaatc tccagcgcgc acgggcgcag gcgccggatc agttcgacga
      601 gatcttccgc gagagtaccg cgacgctgct cgcggaactc tcgaatctga aaacgatcat
      661 cggccggttc agcgagttcg cgaagatgcc gccgccgcag tttgaaattg tcgacctgaa
      721 cgaactcgta caagaaaacc tgcggctgtt cgacgcacag tttcgaggcg aggcccgccc
      781 aaggattcac acggaggcgc acctcgacga aagccttgca aagatctcgg cagatcccgg
      841 gcaagtcttg cgcgcccttc aaaacctgac cttgaacgcc atcgacgcaa tgcccgccgg
      901 gggcacgctt cgctttcgga cgcggcgtgt cgaaggcggc gtgacgctgg aggtatccga
      961 taccggcgag gggctgacga aagaggaatg cgagcggctg ttcactccgt actacaccac
     1021 caaacagcat ggtaccggcc tcggactggc gatcgtgcag tcggtggtca gcgaccacaa
     1081 tggccggatc accgtagaga gcgagcaggg gcgcgggacc acgtttcgca tcgagcttcc
     1141 gaacgctcct gaaaatgacc ttgcgcccga accatcctga catgagccac cttcttattg
     1201 ttgacgacga cccgaacacg ctggcttccc ttgccagggc cttccgcctg gcggggcatg
     1261 aagcgacggt gtgtgacaac gccggccgcg cgctggagct gattcgcgca caacgtttcg
     1321 acctggttct ttcagacgtg gtgatgccgg gcaaggacgg gctcgcactg ctcgaagaga
     1381 tcaagtcaat cgacggggcc attccggtgg taatgatttc gggtcaggcc acggtggaga
     1441 tggcggtgcg cgctacacgg ctcggggcgg tcgatttcct cgagaaaccg atctcgaccg
     1501 ataagctgct gctcaccgtg gagaacacgc tgaaactaac ccgcctcgag gaagagaacc
     1561 ggcagttacg gcgccgtgtg gggaaacagg agatcgtgtg gggcagcgag gccatgcgca
     1621 gtgtgatggc acagatcgaa cgggtggcgg caagcgaaac gcgggtgtgc atcctgggcg
     1681 agacgggcac cggcaaagaa cttgcggcgc gaacaattca cgaccgaagc ccgcggaaga
     1741 acgggccatt cgtcacactc aactgcgcgg cagtgccgtc ggagttgatc gagtccgagc
     1801 tgttcggtca cgagaaaggc gccttcaccg gagcggcctc gcggcacgcc gggcggttcg
     1861 agcaggcgca tcgcggcaca ttgtttctcg atgagatcgg cgacatgccg caggtgatgc
     1921 aggcaaagct gctgcgggtg ctcgagggcg gcgaggtcga gcgggtcggc ggggaccggg
     1981 cgatcccggt ggacgtgcgg gtggtcgtcg ccacgcaccg aaatctgtcc gaactcacgg
     2041 cacaggggaa gttccgggag gatttgtatc accgcatcta cgtgttcccg ctgagcctgc
     2101 caccgctgcg gcaacgcccc gaggatatcc cgctactggc agagcatttc tcacgacacg
     2161 tggcggagat caacggatgg aagccccggc ccttcacggc ggaggcactc gaggaactaa
     2221 aacgctaccc ctggccgggc aacgtgcgtg aacttcgcaa cgtcgtggag cggctgatgc
     2281 tgctggcgga cgaggttgac gcggagacgg tccggctggc cctttccggt cgcgcgccgg
     2341 cggcgctggc ttcggggagc ggcagtctgg ccgaacgggt agatgcgttc gagcgagagg
     2401 cgctgctcgc ggaactccgg cgccacgaat tccgcatggc ggagacagcc agggcgcttg
     2461 ggcttgagcg cagccatctc tacaagaagt gccagcagca cggcatcgat ctgcagacgc
     2521 tgaagaaagt agagtaggcc gggcgtgcgg ttttcggcag atctccagat tgatttcgta
     2581 taattggtcg catgtctggg gtctgggtgc tggtgttgtg ttgtgtcctg acggtcgtcg
     2641 ctccggccca gactacgcat gctctcatct cgggccgtgt ggtggatgtc cagacgggtc
     2701 tcggcatcgg aggagcccaa gtcacctact cggcggcggc gaccaacgcg agcgccacgg
     2761 cgcacagcga cgattccgga tactacgttc ttccgctact tgcgccggga ttttgccgcg
     2821 tcagggtggc agccgacggc taccagcctc aagaactgta cgctctggaa cttccggtcg
     2881 cgggacgcct cgacctcgat ttccggttgc gtttgctggc cgacgtctgg caggcggggg
     2941 aacaccgcag cgtgttcttc ccgaattcgg aagcggtgct caccttcttc ggtccggacg
     3001 tggacaccag ccgctacggc tcgttcgacg caaaccgcgg gcgcaagggc tctcttgagc
     3061 ccacggtttc gcacgtgatc gacccgttcc aagtcagtta ccttccgctg gccggccgtg
     3121 acgtctatac gatgctgctc acgctgcctg gggtcaccgc cgatacgacg acttcccgcg
     3181 gtttgggcct ctcggtcaac ggtcagcggc cgtccgcctc gaattttctg ctcgacggcc
     3241 tggagaacaa caactacctc atcaccggcc cgctgaccgc gatcgctccc gaggccatcc
     3301 aggagtatcg cgtatccacg aacaatttct ccgctgaata cggaaggacc tcgggtttcc
     3361 tggccaacgc cgtcacccgc gccgggggaa ctcagtggca cggcctcgcc tatttctacc
     3421 tgaagaatga tgtcttgaac gcgaatggct tccaggataa ccggaatggc cagccacgcc
     3481 gcccgctgaa ggagaactac atgggcttcc aggccggagg acccgtgcca tctctgccgc
     3541 tgttcacttc cacggcattt gagcgcctcc gcagccgtag cttcggagag cccaccgatt
     3601 ggcttctccc ctcgacccgc ttttcgttgt tcaccacccc cgacagtgtc gccgcaaggc
     3661 tactcgacag gttttctccc ccgcctgcaa ctgacggcaa cctcccctat gcgacactcc
     3721 gactcgttcc tcctctttcg ctggatcgaa ccttggtttt acagcggttc gactaccaac
     3781 cggaaggcag ccgccaccgc ctgatggcca gactggcggc agcgacgctc tcgcggcccg
     3841 atttcatctg gactccgtat cacgattttg tttccggact gtcgcaggac tcagtcagcc
     3901 tcgcctttac gctgaccagc tacctgagcc cgcgctgggc gagcgaaact cgcgccggct
     3961 tgaattggga cgacctccac tgggaccgcc cgcatcccga gatccccact ctggcatcgc
     4021 gagacggggc ggttcttccc ggaagcccgg ccttttacgc ataccgcaac cggacccgcg
     4081 gggcggagtt tgtggaaaac ctgacgtggt cgagggggcg acacgtcttc aaaaccggat
     4141 tcggtgtcct gttgcgtggg ctcgacggct acctcaccgc gggccgcgac gggctctacg
     4201 agttcggcga catcatcgaa tttgccctgg attctccggg tttcttctcc gcctcggttg
     4261 cacgggcctc tctgcccgac cttcaacgtc ccgaattccc gagggaatac gaataccgcc
     4321 agtactacct cttcgctcaa gacgccttcc ggcccacgcg acgtttgtcc ctgagctacg
     4381 gccttcgcta cgagttcttc ggcgcgccgc gcaacacggg ttccgtaaag gacgctattg
     4441 tgaatctcgg caccggcgcc acgttcgcgg agcgcatcgg cagcgctggg ttgaccttcg
     4501 acgggagcgg cgcgcagcgg ttgtacgata cggacgcaaa caactgggcg gggcgcttcg
     4561 gcttctccta cgatctctcc ggcaacgccc gtaccgtcct gcgcggctct tacggcattt
     4621 tctacgaccg gcctttcgat aatctctggc aaaacgtgca aagcaacagc ctgacgctcg
     4681 caagtttctc gcttcctctc tcttcgaggg actacctcaa gcccatcccg gaggcgctgg
     4741 cggagtttcg cgggcgggct ttcagcgcgg attccccagc cctgacgctc gtccagccgg
     4801 gctttcgcga cgcctatgta cacagctact tcctgggtat ccagcagcag gttacagagg
     4861 actgggcggt ggaggtgaac ggcctcgggt cgctggggcg caagttgatt acgaccgatt
     4921 gggtgaaccg ctccttcagt ttcccaccaa gcacttcgaa tcccgatggc cggctcaacc
     4981 cagcgcttcc acccatcttc taccgcgcca accaggggtc gtcgaactat cacgccatgt
     5041 cggccttagt gcgataccgt gcgcgttggt ctcagctaca actcgcctac acgtggagtc
     5101 acgccatcga caatcagagc gagcccctgg cgagactttt tttcgatctg agcgcgacac
     5161 gtgcgacggc cgggtctgga cgaggcatca tctccgcctt cgcgcggcag ttcgacagcg
     5221 gggtcgaccg cagcaactcc gatttcgatc agcgccacaa tctggtcttc ttctcgacgt
     5281 gggaactacc cggctacggg cgcctgttac gaggctggcg tttttctcaa atggtggcgt
     5341 ttcgttccgg ttttccttac acggttctgg ctccctcccg cttttcaccc ggcggggact
     5401 tgatcatcaa caaccgggcc gacatagttg ggcccgcaga aacgacacga ctgccggtgg
     5461 acggcggaca gctcctgctg gaccgctccc ttttccgcga gccacccgcc gggcgccttg
     5521 ggaataccgg gcgcaacgct cttcgcggcc cgggcctgtt caacatcgac gtttcgctga
     5581 gccgctcaac ggcactgccg tggcttggcg aatccggccg cctgacgttg cgcgctgacg
     5641 tcttcaacgt gttgaatcac gccaacctgg gtaacccggc gtcctttctg acctcagacg
     5701 atttcggtgt cgcgctgtac ggacgtcgag gccgcgagtc gacgttcccg gcgctcactc
     5761 cgctggcaga gttggcgaga caggtgcaac ttatcttgcg ggttgagttc tgatgaggcg
     5821 tctcctgttg gccgcggtgc tgagcgtggg cgtgacgcaa gcgcagccgg ccggtgtaat
     5881 tctgtcgatt accggcgggc tcctgcggcg cgcaggaacg cagcttgcgc tgacagcaaa
     5941 atccggggac attcttttcc ccggagacgc tctggcgaca cagaacactc cggcggctat
     6001 cttgttctgt ccggagaaat cactgctcac gctgcaagcg caaaccgaag tgcttgtgga
     6061 tgcgcggcag ctcaagatca gaacaggctc cttggggacc cccaagcccg tcagctcctg
     6121 cttcctcccg cctgtagatc gcaccacgct ggccagccag caacactacg gcgcatcgct
     6181 ggtccgggcg ctgaaacctg agccgccgcc cccgcagttg ccgt
//
LOCUS       DATIFQ010000125         7068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_615667, whole genome shotgun sequence.
ACCESSION   DATIFQ010000125 DATIFQ010000000
VERSION     DATIFQ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615667"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VLE22_05575"
     CDS             <1..640
                     /locus_tag="VLE22_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13907.1"
                     /translation="DEREEFEEHYFSCPECAEEVTTGALLRANTAAVLRDEAQRSTEV
                     PVRSEPGWLAWLGLRPALSAAWAAACVLFAGVAVYQTAFVVPKLNVQLAAARAPQVFQ
                     DVVLRPLTRGEDTVVKAAAKGGYSVLKVHLSPDASFARYRAEILNESGEVLYPIDAAP
                     PAVPGDPLQFLVHSSSVPPGTYTLVVFGRRDPAEPGPGSRLETYRFAVKQDK"
     gene            756..1394
                     /locus_tag="VLE22_05580"
     CDS             756..1394
                     /locus_tag="VLE22_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13908.1"
                     /translation="MTGGTVTAGPVSQTDPVLSFSNARAEIKGWFDSASNTYITENWV
                     RVESLDVEDWLHADRVEVKLTFKFQKSSEQLTADLVTVYSELRVDNAPIASAPPNTLP
                     VHDAENHAQFRNHLAADPGAIGLSGKFKIKKSKAHASLAQNPGLSFVDPNFGFKDSTL
                     HPGRRVYLSEWAEDEDWQGIVGLRAVFLDPQGNLQRQIVVADWLGNNGQFYP"
     gene            1490..3919
                     /locus_tag="VLE22_05585"
     CDS             1490..3919
                     /locus_tag="VLE22_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HSB13909.1"
                     /translation="MRHQNLDAALAHAEQGLAAFKGRRESPWHWRFLLLKAEILSGKG
                     RAADALKLLDHPAPGSPDAELAARLKMQQGYAAFRLNRYQDSLRLLDEALGIAAHVSS
                     PALVAEIQNKRGAPFVLLGRPDEAEQAFRNALEQARRAGDSYLEVSALGNLGSTRLFT
                     ERFDEAIVSFEQMLPLARKGGSPRIIGRTLHNLGICYRSLGDIDRALQCYREAEPLYA
                     QAGDVQPNFDLLGDLGNVYMTTRQYERAADSFRKALDFSRKAGNRLYAAEWLNNLAAL
                     SIELGDLPAAGSYVRESLAALSKIEQPARDKGMLEPLLNSARIEAARGNSSKAEEMYL
                     RAIAGAPKLGAPRVSLEARARLGSLYVRAGRWDRAGRLLRDLASTIESTRSKLKRDEW
                     KLTFQSSIIPFYEEYVSLLMEKGETVRALEVAESGRARVLAEKLDLAGKPQTVTVAAL
                     RNKARALNAILLSFWLAPKQSYLWVVTPSAVRSFTLPPKKEIERLVEACSNLILRMED
                     LRYSENPAGRQLYQTLLAEAERLIPAGSRVVVVPDGALHGMNLEALVTGGVQPRYWIE
                     DVSLAVAPSLALLDTADARAPAGDSLLLIGDPAASSPEFPRLPESATEIRDIRAQFDP
                     ARSTVLTGADARPEEYGRAGPGRFALIHFAAHAVANRESPLDSAVILSGGPDSFKLYA
                     RDVARIPLRAELVTISACRGAGARMYAGEGLVGFSWAFLQAGARNVIAGLWDVSDRST
                     ALLMSRLYAEQRQGKGPADALRAAKLSLLAEPAWRRPYYWAPFELFTRAAPFHSAGIG
                     RRVKRSAASAH"
     gene            complement(4275..5549)
                     /locus_tag="VLE22_05590"
     CDS             complement(4275..5549)
                     /locus_tag="VLE22_05590"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HSB13910.1"
                     /translation="MLLTSRRRFLGRSILLPSLTCTLYEQAGPLLAQQTGASPQYDLL
                     IRGGHVIDPSQDLSADVDIAIREGKVRRIASGIAESEARQVLDARKMIVTPGLVDVHV
                     HVYDGVAPLGIPADPNCIAKGATTVVDAGSAGAHTFPGFRQYVVRMVETRVFALLNIS
                     VVGQSTYSNGNPFGELLDLRYANPKLAIRMIEQNRDVILGVKVRLSRNIAGENDLQAL
                     GLAREAADAVGLPLMAHIGDTHSPLSKILTMLKPGDLITHSYHGRAGGILDDRDRILS
                     EVRKAVESGILLDVGHGAGSFSFRTAEKAFPQDVLPGTISSDIHQGNINGPVYDLATT
                     LSKFLHMGISLEEVIRRATANPAKALVFASGTGSLGVGATADVAVFELREAPWEMVDS
                     MGDKRPGKWRLRPMATVKAGRPYGNSVIPVFA"
     gene            5663..6073
                     /locus_tag="VLE22_05595"
     CDS             5663..6073
                     /locus_tag="VLE22_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13911.1"
                     /translation="MRRRGFLLSLLLGIPALAETGKETVRGTLVQQKDRKPSLRTKEG
                     KTVFLDGDEPTRGVLNDPRLAGSDFEALGHFESPGNFRIDPIHLRAMFVYKDGKKLFI
                     TYWCEVCAIRTYTPGKCWCCQEETALDLREHDQP"
     gene            6073..7047
                     /locus_tag="VLE22_05600"
     CDS             6073..7047
                     /locus_tag="VLE22_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HSB13912.1"
                     /translation="MRPLYAAVLLTSMNIQAADYSARKLTIDSIEIVRLTDTARKIQV
                     SVVPSIGNNAYEMLVNGKNVFWTPFASLAEFKAKPALCANPFLAPWANRIDKDSYFVN
                     GRSYGFNLELGNLRRDPNGRPIHGLLLFSDAWEVVSLKADSAGAEVTSRLEYWRHPAL
                     MAQFPFAHTIEMTYRLKDGGLEVETAMENHAVEPMPVAIGYHPYFRLHDSPRDQWKVH
                     LAAREHLVLSELLIPTGERKPVDLPDPVSLATTQLDDVFGGLVRDASGRAVFSVTGKK
                     EKLSVVYGPKYSVAVVYAPPGREFICFEPMAAVTNAFNLTHAGAYKEL"
BASE COUNT         1381 a         2248 c         2113 g         1326 t
ORIGIN      
        1 cgatgagcgt gaagagttcg aagagcatta cttttcctgt ccggagtgcg ccgaggaggt
       61 aacaaccggg gcgctcttgc gggcgaatac ggcggccgtc ctccgcgacg aggcgcaaag
      121 gtcaacggaa gtgccggtcc gttcggagcc cggctggctg gcgtggctgg gattgcggcc
      181 agctttgtcc gccgcttggg ccgctgcttg cgtgctgttc gccggcgtgg ctgtctacca
      241 gacggcgttt gtcgtcccca agctcaatgt ccagttggcc gcggcgcggg cgccgcaggt
      301 tttccaggac gttgtcctgc ggccattgac gcgaggagaa gacacggtag tcaaagcggc
      361 ggcaaaaggc gggtactccg tactgaaggt acacttgtca cctgacgcta gcttcgctcg
      421 ttaccgggcg gagatcctga acgaatccgg cgaggttctt tatccgatcg acgcggctcc
      481 tcctgccgtt cctggcgatc ctctgcaatt cttggttcac tcttcaagcg tgccgcccgg
      541 cacgtatacc ttagtcgttt tcgggcgccg cgatccggcc gaaccgggac ccggctccag
      601 actggagact taccgctttg ccgttaaaca agacaagtga ggcaagaatg tcaaaccaga
      661 agagattcat cttcagcgct agcgcagtcg cggtagcgga caacgccgag ccccccgggc
      721 aggccgggaa agcctgggcc agtgtcgccc ttcccatgac cggaggcacg gttaccgcgg
      781 gacccgtgtc tcaaaccgat ccagtactct ccttcagtaa tgccagagcc gaaatcaaag
      841 gctggttcga ctctgcatct aacacctaca tcaccgagaa ctgggtccgg gtggaaagtt
      901 tggacgtcga agattggctc cacgccgacc gcgtcgaagt gaaactgacg ttcaaattcc
      961 agaaatcgtc cgagcaactg acggccgatc tggttaccgt ttattcggaa ctgcgggttg
     1021 acaacgcccc aattgccagc gcgcccccga acactcttcc tgtccatgac gcagagaatc
     1081 acgcccaatt ccgaaatcac ctggctgccg atcctggggc catcggactt tcgggcaagt
     1141 tcaagataaa gaaatcgaag gctcacgcaa gccttgccca gaatcccgga ctgagcttcg
     1201 tagatcccaa ctttggtttc aaagacagca ccttgcatcc gggacgtcgc gtctatctaa
     1261 gcgagtgggc ggaggatgaa gactggcagg gaatcgtcgg cctgcgggcc gtgtttctgg
     1321 acccccaggg caacctgcag cgccagatcg ttgtggccga ttggctcggt aacaacggac
     1381 agttctatcc ataatgcggt ggtgtccggc tttggtcctg ttgctggcgg catgcggctg
     1441 ccgtctgggg gaaaagcctg acaagctgta tcaagaagct caacgcgaga tgcggcatca
     1501 gaacctcgat gccgctctcg cgcacgccga gcaaggtttg gcggccttca aaggcaggag
     1561 ggaatcgccg tggcactggc ggttccttct gctcaaagcc gaaatcctct ccggaaaagg
     1621 cagggcggcc gacgccctga aactcctcga ccatcccgcc ccgggctctc ccgatgccga
     1681 acttgccgcc cgcttgaaga tgcaacaggg ctacgccgcg tttcgcctca accgatacca
     1741 ggatagcctg cgtcttctgg acgaagcgct cgggattgcc gcacacgtga gctcgccggc
     1801 gctcgtcgct gaaatccaga acaagcgcgg ggcgccgttc gtcctgctcg gccggcccga
     1861 tgaggcggaa caggctttcc ggaatgcgtt ggaacaagcc cgccgagcgg gtgattccta
     1921 tctcgaagtc tccgcgctcg gcaacctggg atccacgcgg ctgttcaccg aacgattcga
     1981 cgaggcgatc gtcagcttcg aacagatgct gccgctggcg cggaagggcg gctctccccg
     2041 catcattggc cggactctgc acaacctcgg catctgttac cggagcctcg gagacattga
     2101 ccgggccctc cagtgttacc gcgaagcgga gccactctat gcccaggcgg gcgatgtcca
     2161 accgaacttc gaccttctgg gggatctcgg caatgtctat atgacgacgc ggcagtatga
     2221 gcgagccgcc gactcctttc gcaaggcgct ggacttctcc cggaaggccg gaaatcgcct
     2281 ctatgccgcc gaatggctca acaacctcgc tgcgctatcg atcgaactgg gtgatctacc
     2341 tgctgccggg agctacgtcc gggaaagcct ggccgccctg agcaagatcg agcaaccggc
     2401 ccgggataag gggatgctcg aaccgttgct gaactctgct cggatcgaag cggcgcgcgg
     2461 gaattcgtcc aaagcggagg agatgtacct ccgtgcgatc gcgggtgcgc cgaagctcgg
     2521 agcgcctcga gtgtcgctcg aagcgcgcgc ccgcctcggc agcctctacg tccgtgccgg
     2581 ccgctgggac cgggccggcc gcctgctccg tgacctggcc tcgacgatcg agtccacccg
     2641 ctcgaagctc aaacgcgacg agtggaaact gacctttcag tcgagcatta ttccgttcta
     2701 tgaggaatac gtcagcctgc tcatggagaa aggcgagacc gttcgggcgc tggaagtggc
     2761 cgaatccggc cgggcacggg ttctggccga gaaactcgac ctggccggca agccgcaaac
     2821 cgtaaccgtg gccgccttga ggaacaaggc gcgcgcgctg aacgcgatcc tgctctcgtt
     2881 ctggcttgct ccgaagcagt cctacctctg ggtcgtcacg ccgtcggccg tcaggagttt
     2941 caccctgccc ccgaagaagg aaatcgagcg cctcgtcgaa gcgtgcagca acctgatcct
     3001 gcgaatggag gatctccggt attcggaaaa ccccgcgggc cggcagctct accagacgct
     3061 gctggccgaa gcagagcggc tgattccagc gggatctaga gttgtcgtgg tccccgacgg
     3121 cgcactccac gggatgaacc ttgaggcgct cgtgacgggt ggcgtccagc cccgctactg
     3181 gattgaggac gtgtcgctcg cggtggcgcc gtcgctggcg ctactcgata cggcggacgc
     3241 gcgcgcgcct gccggcgaca gcctgctgtt gatcggcgac ccggcggcgt cctctccgga
     3301 gttccctcgg ctgcccgaat ccgctacgga gatacgcgac attcgcgcgc agtttgaccc
     3361 ggcgagatcc accgttctca ccggagcgga cgccaggccc gaggagtacg gccgcgccgg
     3421 gcccggccgc ttcgcgctga ttcacttcgc cgctcacgcc gtcgccaacc gggagagccc
     3481 gctcgactcg gcggtcatcc tctccggcgg tcccgactct tttaagctgt acgcccgcga
     3541 cgtggcccgc atcccgcttc gggccgagtt ggtcacgatc tcggcctgcc gtggggctgg
     3601 tgccaggatg tacgccggcg agggattggt gggcttctcc tgggcgttcc tccaggccgg
     3661 ggcgaggaac gtgatcgccg ggctttggga tgtgagcgac cggtcgaccg cccttctgat
     3721 gtcgcggctc tacgcggaac aacggcaggg caaaggtccg gcggacgctc ttcgcgccgc
     3781 caagctgtcc ttgctcgccg agccggcctg gcggcggccc tattattggg cgccgttcga
     3841 gttgttcacc cgcgccgctc cgttccattc cgcgggaatc ggccggcggg tgaaacgttc
     3901 ggccgcgtcc gcgcactaaa gggttggaag ccgaaaccgt gaggcggcgc agttgctctc
     3961 aggagcaccc tcggtttttc cccacaacga ccgcagtacc aacctcgaaa cggcttgccc
     4021 gccctagcat ccgcgccgcc tccgacactc cgtctgctca atcttcccgt ggcgcagaaa
     4081 aattctcctt cgtctgaaac gccgcggccc gcaatgtcac taacagggta agaatcgaga
     4141 tttcttgttt cttcgccggt acaagaaatt ctcattcgag ggccatggcc aacggagggg
     4201 ccgcggccct cttttttggc ctcgttcggg ggaactgtgg gcgactgtga tgtttcgaag
     4261 agtgggccgg cggattatgc gaagaccgga atgacggagt tcccgtaagg gcgaccggcc
     4321 ttcacagtag ccataggccg cagacgccac tttccaggcc gcttgtcacc catagaatcg
     4381 accatttccc acggcgcttc gcgcagttcg aaaacggcga catccgcggt tgcccccaca
     4441 ccaagcgagc cggtaccgga agcaaacacc agcgcttttg caggattcgc ggtcgcccgc
     4501 cgaattacct cctccaaact gattcccatg tggaggaatt tggataacgt tgtcgctaaa
     4561 tcgtaaaccg gcccgttgat attgccctgg tggatgtcgc tagatatcgt tcccggcaag
     4621 acgtcctgcg ggaacgcttt ctccgcagtc cggaaagaga agcttccggc gccatggccg
     4681 acatccagca gaatgccgct ttcgacagct ttacgcactt cggacaaaat gcggtcacgg
     4741 tcatcaagga tcccccctgc acgaccgtgg taactatgag taatcagatc tccgggcttc
     4801 agcatcgtta gaatcttgga cagcggcgaa tgtgtgtcgc ctatatgcgc catcagcggc
     4861 aggccaacgg catcggccgc ctccctcgcc aagccgagag cctgaagatc gttctcaccg
     4921 gcaatattcc tgctgagacg aaccttcacc cctaagatca cgtcgcggtt ctgctcaatc
     4981 atgcggatcg cgagcttggg attggcgtag cgtaagtcca acaattcacc gaatggattc
     5041 ccgttggagt aggtggactg acccacgaca gagatattaa gtagcgcaaa gacccgggtt
     5101 tccaccatcc gaacgacgta ttgccggaac cccgggaacg tgtgggctcc ggctgaacct
     5161 gcatcgacca ccgtagtggc acccttagca atgcaattcg ggtcggcggg tattcccagc
     5221 ggggcgacgc cgtcatagac gtgcacatgc acatcgacca gcccgggtgt cacgatcatt
     5281 ttcctggcat caagcacttg acgggcttcg gattccgcaa ttccgctggc tatgcgtctg
     5341 accttcccct cacgaatcgc aatgtccaca tccgcggaaa ggtcttgtga ggggtcgatg
     5401 acatgcccac cccggatcag gagatcgtat tgcggggagg cgccggtttg ctgagcgagg
     5461 agcggtccgg cttgctcata gagagtgcat gtcaaagagg gaagtagaat actccgaccc
     5521 aggaaccgcc ggcgagacgt gagtagcatg agtccaaggg tatttcaggc atttggcctg
     5581 tcgcaagctg gaatcgtcaa cgtgtccgct gcgcacgaat tgccggcagg cacgcgatcg
     5641 acgtcgcgat agaatgaaag tagtgagacg ccgagggttc ctgctcagcc tgcttctggg
     5701 tatccccgca ctggctgaaa ccggaaaaga gaccgtgcgc gggacactcg tccagcagaa
     5761 ggaccgcaaa ccgtcgctga gaacgaaaga gggcaagacg gtttttctcg acggcgacga
     5821 gccgacgcgg ggtgtgctaa acgatccccg gctcgcaggt tccgatttcg aggcgctcgg
     5881 ccatttcgag tcgcccggga acttccggat cgaccccatc catcttcgcg ccatgttcgt
     5941 ctacaaggac ggcaagaagc tcttcatcac ctactggtgc gaagtttgcg ccattcgaac
     6001 gtacacgccc ggcaagtgct ggtgctgcca ggaagaaacc gccctcgacc tccgcgagca
     6061 cgaccaacca taatgagacc gctctacgcc gctgttctgt tgacttccat gaacatccaa
     6121 gccgccgact actccgcccg gaaattgacc atcgattcca tcgaaatcgt gcgtctcacc
     6181 gacaccgcgc gcaaaatcca ggtatcggtc gtgccatcca tcggcaacaa cgcctacgag
     6241 atgctcgtca acggcaagaa cgtcttctgg acgccgttcg ccagtctcgc ggaattcaaa
     6301 gccaagccgg cgctgtgcgc caatccattc ctggccccat gggcgaacag aatcgacaag
     6361 gattcctact tcgtgaacgg acgcagctac ggcttcaacc tcgagctcgg caacctgcgc
     6421 cgcgacccga acggcaggcc cattcacggc ctgctgctct tctccgacgc ttgggaagtc
     6481 gtctcgctga aagcggactc cgcgggcgcg gaagtgacca gccgcctgga atactggcgc
     6541 catccggccc tcatggcaca gttccccttc gcccacacca tcgaaatgac ctaccgtctg
     6601 aaggacggcg ggctcgaagt ggaaaccgcg atggaaaacc acgccgtcga accgatgccg
     6661 gttgccatcg gctaccatcc ttatttccgc ctgcacgact ccccgcgcga tcaatggaaa
     6721 gtgcacctcg cagcccgcga gcacctggtg ctttccgagc ttttgattcc gaccggagag
     6781 cgaaagcccg ttgatcttcc agaccccgtc tctctcgcaa cgacgcaatt ggacgacgtc
     6841 tttggcggtt tggtgcgcga cgcgagcggc cgcgcggtct tctccgtcac cgggaagaaa
     6901 gagaagttgt cggtggttta cggcccgaag tacagcgtcg ccgtggttta cgccccgccg
     6961 ggaagggagt tcatctgctt cgagccgatg gcggccgtca ccaacgcctt caacctgacg
     7021 cacgcgggcg cctacaagga actctagagc atcccgcccc ggcagacc
//
LOCUS       DATIFQ010000126        36391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_616756, whole genome shotgun sequence.
ACCESSION   DATIFQ010000126 DATIFQ010000000
VERSION     DATIFQ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 36391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36391
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616756"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VLE22_05605"
     CDS             <1..486
                     /locus_tag="VLE22_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB13913.1"
                     /translation="HLDTFRIIRQQCSTPLAMGEVYTGQWDGLFLVTEHLIDYVRHDL
                     VHCGGITAGKKVATMCEPYGILTAWHGPGNISPVAHMANCHVSYSVPNFGIQEYSTGW
                     SPAIREVFTAVPAYRDGHVTIGDAPGLGIDVNEAAAKKYPYLRRMRPTIRRADGTAWP
                     Y"
     gene            763..1146
                     /locus_tag="VLE22_05610"
     CDS             763..1146
                     /locus_tag="VLE22_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB13914.1"
                     /translation="MSSCVLDASAVLAYLHREPGHDRIERLMEGGAVSAVNVSEVASK
                     LIDRGVREETVREIVGDLGLSVIPFDEALAYRAASLRQPTEALGLSLGDRSCLATAAL
                     LGVRAITADRAWEKLKLPVRVLVVR"
     gene            complement(1167..3203)
                     /locus_tag="VLE22_05615"
     CDS             complement(1167..3203)
                     /locus_tag="VLE22_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HSB13915.1"
                     /translation="MRRGLRLLPAVSLSSLLLLFGCAFRGPDSARVDGPGIRIEFNQA
                     THSRVIAKFGLKEVPIGPFSASETLTAGGKEIRDFRMSGDRIEQRIGEKLYIITGEVP
                     GVRKEVSVTVSDRFPQMALFEVRYTNTGSGDLTVGGWTNHHYVIDAPPGAAEPAFWSY
                     QSGSYENRPDWVLPLKAGFHQENFLGMNGTDYGGGTPVADVWRRDAGIGVGHVELVPK
                     QVSLPVEMPGAGHATVAVRFKTNRTLKPGETLKTFRTFVAVHNGDYFQTLAEYRRFMI
                     SQGVRFPVPPADAYEPIWCAWGYGRNFTPKQVYGTLPVVKKLGFRWVTLDDGWQTAEG
                     DWYVNRKKFPAGDRDVRKMVDRIHAEGFKAQLWWAPMSVDPGTDLIKARPEQLLLNAD
                     GSRQKISWWDAFYLCPAFPPVRQDAAAFAVKALRDWDFDGLKIDGQYLNAAPPCYNPA
                     HRHAAPEEAVEGVPGFFKAIYDAAVGVKSDALVEICPCGTAYSFFTMPYMNMAVASDP
                     GNSWQVRTKGKTLKALLGDSATYFGDHVEMSDGGDDFASTVGIGGVVGTNFTWPPKVG
                     KDRKVWLTPEREKHWARWLEIYKTKMLSKGEYLGSLYDIGFDRPEAHAIRKDGVMYYA
                     FFAGNWKGKVELRGLDRRPYRVTDYVGGKDLGTVTGPVASVDVAFEKHLLLEAK"
     gene            complement(3325..3705)
                     /locus_tag="VLE22_05620"
     CDS             complement(3325..3705)
                     /locus_tag="VLE22_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13916.1"
                     /translation="MQKRVHKRVLAMVSDLFFTVKISDAAKRAGMDVEFVKDDKELIE
                     KAQSKPEVIILDLNFSAAQPLKVISKLKGNSDLKATSLLAFVSHVHGELKQKAHENGA
                     DLVMARSAFSQNLPQILKRHAGTP"
     gene            3937..5469
                     /gene="zwf"
                     /locus_tag="VLE22_05625"
     CDS             3937..5469
                     /gene="zwf"
                     /locus_tag="VLE22_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSB13917.1"
                     /translation="MSEGNPFHDPLRFERHAPPCALVIFGANGDLTRRMLMPALYRLA
                     YDRSLPPGFTVVGNSRSAMSDERFRDVMRESVIQFLRDTPFDERIWRDFAEGLYYRAG
                     DLRDQGFYRELEKVLVSAENERHSGGNVVFYLATQPSLYEAAAAGIGAAGLQQSAGWR
                     RLVIEKPFGRDLDSARDLNRKLHAVFPEESIYRIDHYLGKETVQNVLAFRFGNGVFEP
                     LWNRRYVNHVQITAAESIGVEGRAAYYQESGALRDMIQNHLLQVMATVAMEPCAVYEP
                     NAVRDERAKLLRSIRILREDEVDSHAVAGQYGPADIGGEQARGFRQEDGANPDSQTDT
                     YAALALFVENWRWAGVPFYIRTGKRMPRRVTDIAISFNTAPFSLFGSDGQTGLMATTP
                     NLLVLRIQPEEGISLRFLSKQPGGGMRLRPVSMDFNYGSSFGARLPSAYETLLLDVMT
                     GDATLYTRQDMVEASWAVVQPVLNAWAGRRFDFPNYAAGTWGPAAADEMLACHGHAWR
                     KP"
     gene            5469..6467
                     /locus_tag="VLE22_05630"
     CDS             5469..6467
                     /locus_tag="VLE22_05630"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HSB13918.1"
                     /translation="MAAVVNPERLLRELHGLWIDLGREAEADGGPGVIRACALTLIVM
                     TEDAAGVAETLAEVMREHPCRGIVVSVRPDASSVDARVSAQCWLPLGQKRQICSEQIE
                     ITTSGPTLGHVYPLLNALTAPDLPVVVWYRGASLFRSSELRLPLSMAGRLILDSGQIE
                     APGAFLRELIEVPHNGLRAGDLAWTRLTCWRDKIAQLFEAHVCRDGSTGMSEVHILWE
                     GRTARSDVVYMAAWIAEALGWEPQDSRLCVERARGAGDGRLLGVRLAAPGMTVAIARE
                     DGGKVAVTLNGMLHEAFFPERSEASALTEELSIVERDAVYEHVLPLAARLVRPGLE"
     gene            6471..7190
                     /gene="pgl"
                     /locus_tag="VLE22_05635"
     CDS             6471..7190
                     /gene="pgl"
                     /locus_tag="VLE22_05635"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSB13919.1"
                     /translation="MDARRHCFPTPQLAAEGCARHVGSLIEETFAEQSRATLAVSGGN
                     TPKLLFEHLAVSSLPWDRVHVFWVDERAVSPADPRSNYRLAEESLIHPARIPERNVHR
                     IHGEWTPDLAARRYAGELREFFGLSEGEMPVFDVILCGMGSDAHTASLFPGEPAILDR
                     ERIAAAVRVTKLPQSRITLLPGPLLAARHLVFLVTGADKAEAVRAVFQEPPDPLKRPA
                     QLVRDRRTTWFLDDAAASLLE"
     gene            7197..8180
                     /locus_tag="VLE22_05640"
     CDS             7197..8180
                     /locus_tag="VLE22_05640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HSB13920.1"
                     /translation="MTTRRNFVLLPAATLLCGRRPPSGDPRPEPLPATRLVFGGDVML
                     SRFVGRLARSQRDPTWPFQALAPYLAGADIAFLNLESPFSDRGWPVEKGMVFKAEPEM
                     IDGLLMAGVDVVSTANNHARDCGAYGIEFTLGWLARHGVAAAGTGMTEEEARQGAVLE
                     RNGVRFGFLAYTYDQSNGNHPKPDPRVAMLDLERMREDIAGMKARSDVVIVSMHAGVE
                     YRPKPNEQQLRFARAAIDAGARLVVGHHPHVVQPVEKYGEGVIFYSLGNLIFDQFHRR
                     ETQRGLLSEVTFRGKWLERVELRDVEIPRGAPQLSGLPSVFLERSIAPAGG"
     gene            complement(8158..9243)
                     /locus_tag="VLE22_05645"
     CDS             complement(8158..9243)
                     /locus_tag="VLE22_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HSB13921.1"
                     /translation="MNYYEELGLAENASADEIHRAYRNLARLLHPDQQPDESVRRLAE
                     VQMKRLNRIHETLGDPVERRKYDLSLRDGESGGALHPGLVALRPACAFPRRRRGLAAG
                     DAIWVVTALIAGAAMCGYVGTQASPPPQPASAVGKTSADPKASAMPPRAVMPDTARRR
                     QVAALSSELISLRRELEAARSERDTALAQLAEHDLQAREEFRRAGEDTSAPPAHAPPA
                     GRSLASASPPISGIPPPDEIHGRWTGTWVYVPLPLTSDSMALYPPDYIEMVISEHNGS
                     IRGRYRARYVITDRAIPSEVRFQFEGEAERDAAHLNWMGVGGARGEVRLRLVSDHSLE
                     VNWTALELGRQMGLVSGTSVLTRRLEQ"
     gene            9353..10498
                     /locus_tag="VLE22_05650"
     CDS             9353..10498
                     /locus_tag="VLE22_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13922.1"
                     /translation="MRNRDSEGAQASRVLPYLEGRRGGQTAPRLFSSTRIEDWLARAP
                     IVVTEGPERGGVVGWLDEDDVAPFVYGEASGYYLTFLTWLARETGISFTTWARREAVL
                     GWCRRWTQGNRVPPTRCILDGYAADWRNGFQFSFDLAMVLRGLVHAGCESPARSLLAY
                     LCRFVSPAGTLRVALPLNKDCADVHHWSVEPGPCQLKTAAAILSASDFGIPEALRVAV
                     GRSIDCWRRTTDPSGEAHPFLYFIEGLILCGYYLGDRSLWRTAAETYARLLDHQTADC
                     GLPASVPDSGSVQGSDVIAQALRIGCLLRHLGHLKPYFDTRLEGLASALDRFLLADGA
                     VSFLDPRLAARTHRNTWCAMFAHQAYCLYGRFLRGAAMPASFLDVMV"
     gene            10501..14190
                     /locus_tag="VLE22_05655"
     CDS             10501..14190
                     /locus_tag="VLE22_05655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB13923.1"
                     /translation="MEPPEVPPADEIPCDPPRVLWIELTSRCPFECIFCSRKTLRGAG
                     RHMDFGLCRRILDQLRNPEVIRLNYSGESIHYPRLAEAIELAHATGARTELVSALASA
                     PEHLLEALVTRGLDRLTVSLHTTASNQFEEIYRYSSLDNLRAKLALLLRLREERRTAK
                     PSLDFAFVAMRRNLDQLLPIAEFARECGVREIRVFPVIRRDEIPHRFDQELEDGRLRT
                     SFQREILETVERVRQLCPDVQFGVTPAETLDEPALGPQPQPYSGALPPGGRIYSCEQN
                     PWETAHILANGDVVVCEVQDKVSMGNLNDVPLEQIWHGPKYSAFRRRYADGEDPVCCR
                     CPYKIAYAPSAMRCCVEAGDGMSFQLARGWHLPAHEDAIWSKWHCCMVLKGNGFGAKL
                     QIRGILPPARDASSNTLSLRAGGRFLGSIRNPSGDMLAFDSTFTLPGDAGTVTIECST
                     SSVCRPAEQGASRDERELGFALIRVAVEKKESRWLRWALYAADFLVPFARRLRPFRRR
                     LSAAWSPGISIVIPERGSPEMLRECLSGVRAAAGQALEPVEILVVVNGAPLLQYREVA
                     REFPETIWLTSPRPLSFAQAVRRGVRRARYDWVFLLNSDAVPEPDALAQLLPWRGPHV
                     FAVASRIVLPGASAHPEETGWTDFRQVRGEVEIFDVAPEDEETVRGSLYAGGGASLFR
                     KALLRRFLARRDPYAPFYWEDVEWGVRAWRAGYEVLFAPKSRVRHRRRATVSRYYKDH
                     EIDRIFRRNQIQFMLRNRFDDGDAHRLLAGLVKEDPTTQAAILKPGNCLRLLWARVRT
                     SAARNANFCFPYVRRKFYPTPFQSLPEGRAILVASPYAVFPPAHGGAVRLARLLEHLK
                     RQRKIVLLSDEESCYQEQALPYFAGLAAVHLVGGRREMCEQRADRIGRILSHSHDILK
                     REATRLAESYSVGAVQVEFVELAKLVEAREGAAPWFLTLHEVLLESGGRPEEDSFEKE
                     WIARYDAAIVCSPEDAALLHHREVLVAPNGVEMRGDVYVPSRGRRTIAFLGPFRYRPN
                     WDGIQRFLTCVYPRLLRTLPGVGIDVFGGLGALERAAGVDCFRQPGVRVHDHVEDVRR
                     RLDACAVTINPLDQTRGSCLKVIESLAAGRVCVSTVAGARGFLRDGFRSLVAVPRVGD
                     FLEPLGLLLSEEDRRLELEPPQAELLDRYRWEHAARKQLDLYRRYREASQQAPMWRSL
                     YRLLKNPLDGSATYPASGAVVDRKL"
     gene            complement(14198..15226)
                     /locus_tag="VLE22_05660"
     CDS             complement(14198..15226)
                     /locus_tag="VLE22_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSB13924.1"
                     /translation="MKVARIHQHGGPEVLRYEEAPEPQIRADQVLVRVKACALNHVDL
                     WVRAGIPGMEFSMPHVLGSDIAGEVIAVGEYCERIRTGQRVLLAPGLSCRQCPQCVSG
                     RDNHCRRYTMFGYGVDGGNAELMAAPEYTAIPIPDDLSFEEAAAVPLVFLTAWHMFFD
                     RAKLQPGEEVLVLAASSGVGSAAIQIAKLFRCRVIATAGSEAKLAKAKDLGADEVIDH
                     YRQDVAAEVKRLTGRRGVEVVFEHVGAATWSKSTQALAPAGRLVTCGATTGFDVKVDL
                     RFLFNRQQSLLGSFMGSLGELHQVLQFVFRKQVHAVIDSIYPLSEIAEAHRRLEGKEQ
                     FGKIVLRV"
     gene            complement(15292..15972)
                     /locus_tag="VLE22_05665"
     CDS             complement(15292..15972)
                     /locus_tag="VLE22_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13925.1"
                     /translation="MPRESRLSLILIVLIVCAFLAAPAGATCVLYTDRITWEAATSGI
                     FTVDFEGLATAAVPGNYSTAGGYTIGGLQFIGLTPPASYYLYAQDPASSQNYTWGSGD
                     VLMGPLAAWGGGYIQVNMPSGGVTSFGTNIMTHTPFAAQIVVTLSTGDVRTVNTFSNP
                     NPAFVGFTSDVPITFVRLAPVNGAPMIDNFSYGAAMQETPEADSLILAGAGLLSILLG
                     QRLRRRAA"
     gene            complement(16092..18770)
                     /locus_tag="VLE22_05670"
     CDS             complement(16092..18770)
                     /locus_tag="VLE22_05670"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HSB13926.1"
                     /translation="MPSSQLTRRECAKNLILGATPLVLRGAAKEHTIENEALKFSVGL
                     GDGVLVSRSFTNKLSNEAAQLPRRDFLFEFDGGETVDCADLSGKVTRAAADRIELVFS
                     RTSGAMAGLEAKVEYHLPAGKAYLRKQISLRRGSGPVARLLRADLDNWQGVRRDWQSL
                     HADRRPFGSHPIFCDSLWAGVEFVAAFNEYGPDGFVLRSRPGGMQIGSDWRPLRSTVA
                     GCAAPRRVREAFLRYIDDIRVAPPRLVACYNTWWTFYAKDLSAERYQALAEELKRQLR
                     DAHGLFFDVVATDEGWALPQSIWEMDRRKLPNGFDRVREVVESAGGKMGLWMSPSAVY
                     PNSTDYDWAKSQGHVVLEYHMRPNQRRPGISLADPAYREKTKTHLKKLIEDSRLEHVK
                     YDGFIAFEKQGHHDLLPGDDSVEPLASHMLELIEASKAANPNLVAEPTCMNSHVNYIS
                     PWIVKHADVIWGNAGGDCPPGLGPAPDYREAHTTAREYYIFSSLNEVWLPQNALQYFD
                     IVHCDDSAGFANHAAMAFGRGRFFVPTYVNPKYMTADDWRIYAGLLRWARKNTDLLRN
                     TVVLPSRVELGEPYAYAHWLGARGVIAVRNPSNETREYILDLERSGAPRELTDAVCYT
                     QYPYRKGIAAGVTSASTITLRLAPWELLFVEVVPRAQLREPVIVGGRWYADAGGQAFA
                     SPDFEVESVRVLEPGGRDSVTKTTASTRGDFRGSLISRTVRSIPEGEWLKDKDKPVPT
                     AGFELECDVSVPTGASGAKMFFLAEFPGRVHHASTCRITVNGKPVEPGVTSSEGHIGQ
                     YSASPDSYWKDVLKHESVWTWYTIEIGAGSSRIKFDGMAADRRCRIGLWSWADFDTRA
                     RQVSLPVAARMPEMPQHSEHIERHGICIVPPAPVLG"
     gene            18936..21992
                     /locus_tag="VLE22_05675"
     CDS             18936..21992
                     /locus_tag="VLE22_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13927.1"
                     /translation="MGILGEVLALTLLGVSAVEAQQYDISTVVGGSPPPTPIAGVHVS
                     IGFPLGTATDTAGNLYFTALNCVFRLDSNGVMTHVAGNSRAGYSGDGGPATSAQLDST
                     SKGFDWPVPAGVAVDASGNLYIADSSNHRVRKVSAAGIISTVAGDGSPGYSGDNGPAI
                     SAQLGVPSGVAVDAGGNLYIGDTGNYRIRKVSAAGIISTVAGDGSPGYSGDNGPAISA
                     QISRPYGVAVDAGGNLYIADADNNRIRKISVAGIITTVAGNGSAGYSGDGGPAISAQL
                     DYPWGVALDASGNLYIADLRNSRIRKVSVARVITTVAGNGSAGYSGDGGPAVNAQFFN
                     LYAVAVDASGNLHIADTGNSRIRKVSAAGIVTTTAGNGSLSYSGDGGPAASAQLSQPR
                     GVAVDAGGNLYIADTDNFRVRKVSATGIITTVAGNGSRGYSGDGGPATSAQLLNLYAV
                     AAGASGNLYLAGIPHIRKVSAAGIITTFVGGDSPGYSGDGGPAANAQLNFPNDITVDA
                     GGNLYIADQGNRRIRKVFTTGIITTVAGNGSAGYSGDGGPAASAQLSQPRGVAVDAGG
                     NLYIADTDNFRIRKVSTAGIITTVTGNGSAGYSGDGGPGVDAQLNYPNGVAVDAGGNL
                     YIADEGNNRIRKVSATGIITTVAGNGSRGYSGDGGPATSAQITFPSDVAVDAGGNLYI
                     TDYSNNVIRLLTPTSSTCTYTLSATSLQSTVLGGSSTLTITTESGCGWAISNLPDWIT
                     ISGSATGSGSASITLVVAPNFFAPRAAQIFVAGITVTVYQASNVLWISPGGVVSAASY
                     TAPVAVGSIAAIFGNFLLPEPASVSSFPIPTDLGGLSLLFSGARLAPLFYANVGQVNA
                     QVPWELAGLPQTTITAALDGQTSVPQTVTLATFAPGIFATNSGGTGQGAVLDENYHLV
                     DPTNPATAGSTVVQIYCTGLGPVTNRPATGGPAPRNPLAETTTQPEVTIGGARAQVWF
                     SGLAPGYAGLYQVNAQVPQGAAKGHAVPVVISIGGVQSNTVTIAVQ"
     gene            complement(22125..23495)
                     /locus_tag="VLE22_05680"
     CDS             complement(22125..23495)
                     /locus_tag="VLE22_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSB13928.1"
                     /translation="MRLLCSLLLLAWCAGAETADWIWTARYVVTMDPARRLIEDGAVA
                     IRGDRIAAVGTRTDIERRFRASSRIDLPQGLIAPGLVNTHTHAPMSLFRGIADDLRLQ
                     EWLEKYIFPAEAKNLSPDFVRWGTRLACLEMALSGTTTFADMYYFEDVIAEATKEAGL
                     RGVLGETILDFPAPDAKTPEDALAFTERFIRRFKDDSLIVPAPAPHALFTNKDETLKA
                     ARVLANRYGVPLLTHLSETKQERDDAVTKRGMSPVRVLDSLGVFNGRTICAHSVWVDD
                     ADLGILKKRGVGIAHCPSSNMKLASGVAPVVKMLSMGIAVGLGTDGPAGSNNDFNLFE
                     EMDLASKLQKVISGDPRALPARQAFEMATITGARVLGLEKEIGSLEAGKRADLIALRL
                     DRPHAVPLYDVYSQLVYALKGSDVEHVMVNGRMIVRARRMLTLDEARVLAKAREYAEK
                     VRRSLR"
     gene            complement(23479..23862)
                     /gene="cdd"
                     /locus_tag="VLE22_05685"
     CDS             complement(23479..23862)
                     /gene="cdd"
                     /locus_tag="VLE22_05685"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HSB13929.1"
                     /translation="MDPLVDAAVSARENARASFSGFRVGAAIEDAEGRLHTGCNIENA
                     TLGLTVCAERVALFKALSEGVRTFRRIAIATDSETLTPPCGACRQLLWEFCGDVEVIL
                     ANLRGKTESLRLKDLFPRPFDASSL"
     gene            complement(23862..24671)
                     /locus_tag="VLE22_05690"
     CDS             complement(23862..24671)
                     /locus_tag="VLE22_05690"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HSB13930.1"
                     /translation="MYQAAIDEIRSRTPLRPRIAVVLGSGLGAFAEELSGRTEIPYGK
                     IPGWPHSTAIGHAGMLVFGRLGGIEVAVMSGRVHLYEGYSPDQVVFGVRVLGKLGVTA
                     LVLTNAAGGIDLSLSRGGLVLISDHINLQGSNPLVGPNDESLGPRFPDMSVAYSAGYR
                     EIAKETAAELGIPLGEGVYAGLLGPSYETPAEIRHLRAIGADLVGMSTVPEVIAANHM
                     GMKVLAISCVTNMAAGILPQKISHEEVLETGVMVRGTLVKLLTALLPRLEV"
     gene            complement(24675..25898)
                     /locus_tag="VLE22_05695"
     CDS             complement(24675..25898)
                     /locus_tag="VLE22_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HSB13931.1"
                     /translation="MGRFSGLIGLVVILGAAWVFSAKRRAIRPKLLLWGLGLQFAFAF
                     LVLKTGFGRVFEAASVAVNALLAYAEAGSQFVFGPLGAKSGPFGVVFAFQVLPIVIFI
                     ASLFSVLYYFGVMQVFVRGMAVLMQRVMGASGAESTNVAASIFMGQTEAPLTIRPFLP
                     RLTQSELFTVMASGMAHVSGAVMAAYVKIAGVDIKHLLTAVIMTAPATILLAKMLIPE
                     TEQPETAGKVRVEIEKPGVNVIDAAARGAGDGLHLALNIGAMLIAFLALIAMVNGMLG
                     WTHGLPGMGWLPESLEKIFGVVFAPIAWLMGVSWKDATSVGNLLGTRLVLNEFVAFLQ
                     LGPIRDQLDPRSFTIATYALCGFANFSSIAIQIGGIGALAPGRKSDLARLGLRAVAAG
                     TMANFMSACIAGMLL"
     gene            complement(25909..27213)
                     /locus_tag="VLE22_05700"
     CDS             complement(25909..27213)
                     /locus_tag="VLE22_05700"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HSB13932.1"
                     /translation="MRIVDLIQRKRDGEELAPNEITFLVDGYTQGEVPDYQMSAFLMA
                     VYFSGMSDREVTALTDCMLRSGESVDLSSVPGVKVDKHSTGGVGDKTSLIAAPLAASA
                     GAIVPMISGRALGHTGGTLDKLESIPGFRTNLTLDEFRAQLQQVGIAFIGQTAEVTPA
                     DSKLYSLRDATATIESIPLIASSIMSKKLAAGLDAIILDVKVGAGAFMKRQVDARRLA
                     QMMVGIGRRMDKRIQALITDMNQPLGYAVGNALEVMEASQTLQNVGPTDLTRISLELA
                     ARMIYLGSITETLDDARELAQQKLLDGSGYRKFREVIEAQGGNPQVLDRFELLPNASG
                     AREITSPRAGFVSAIDAEDVGKASAMIGAGRDNKDDQVDPAVGVILEVKAGEKVEAGA
                     VLCRIYYTGDERLDDAAAVIEDAFRISNQAPEERDLILEVVG"
     gene            complement(27351..28160)
                     /locus_tag="VLE22_05705"
     CDS             complement(27351..28160)
                     /locus_tag="VLE22_05705"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSB13933.1"
                     /translation="MTSLLDSAKEIAREAGALLHNYYERRVTFELKGEFDLVTEADRA
                     SERLVVERLRSRYPTHGIVAEEGGGQSGTSEYRWYVDPLDGTTNFAHGFPMFNVTLAV
                     ERSSELIAGVIYDPLRDEMFAAEKGGGAYLNNHRIRVSKAARLQDCLVATGFPSRKRH
                     LSVNVHFYHQLAMATHGVRRGGSAAIDLAYVACGRLEGFWEFGLNPWDMAAGVLLVAE
                     AGGTVSDMDGANHNLSSPHLLADNTAIHSEILGLFEEIFAGKYRVPLPQIG"
     gene            complement(28183..28428)
                     /locus_tag="VLE22_05710"
     CDS             complement(28183..28428)
                     /locus_tag="VLE22_05710"
                     /inference="COORDINATES: protein motif:HMM:NF028518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HSB13934.1"
                     /translation="MPKKENGKSKAKPPEQTFEEPKYLRRLIENAIPITVRLQSGEEY
                     SGTIEFYDANFIRLTRQDGPNLFLYKHDIKYLYEDEE"
     gene            complement(28443..29414)
                     /locus_tag="VLE22_05715"
     CDS             complement(28443..29414)
                     /locus_tag="VLE22_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB13935.1"
                     /translation="MGRIRPQKVVALAGAALLLAGALLLAQETVFRVDVSLVRILATV
                     KDSAGGLVGSLEKADFTVIDSGVPQEVSVFERRTEQPLSISLLVDVSLSTAKELRYEL
                     DSVNRFLKAVFSEGHPEDAVALYGFSYEVTLHARFTNRHERLEDAMKSLKPESGTSLY
                     DAIYLASDALDQREGRRVIILVTDGGDTTSAKNFHQALEAAQRSDAVLYAILVMPITN
                     DAGRNIGGENALTLLSSGTGGRVFTPSVGPALDSAFSDILKELRTQYFLAYYPKNLPP
                     SQDRYHRIEVKVSRRDLRVLARNGYYEETDSSSKRRPRGWRPVEKSP"
     gene            complement(29387..30103)
                     /locus_tag="VLE22_05720"
     CDS             complement(29387..30103)
                     /locus_tag="VLE22_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HSB13936.1"
                     /translation="MRVKVLFFGVLKEATGCSEQQIELSDGSEVGAAFEHFARRFPNL
                     AEWKASVVPAVNQHFSQWNQRVADGDEIAFLPPVSGGAGFARVVEDSETGHFFALTRE
                     PIDARALAARLLRGEDGAVVTFEGVVRNNTKGRPTLYLDYECYEPMAIGVMARLGREI
                     AASHAISHIGMIHRLGRMEIGEASVVIVVTAPHRKPAFEAALEGINRLKRTVPIWKKE
                     HFADGETWVEGEWDESVLKR"
     gene            30166..31722
                     /gene="garD"
                     /locus_tag="VLE22_05725"
     CDS             30166..31722
                     /gene="garD"
                     /locus_tag="VLE22_05725"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR03248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HSB13937.1"
                     /translation="MPPLTVRVHPDDNVAIIVNPEGLPAGTVFPDGLTLAEAIPQAHK
                     VALRDLDAGTPVVRYGHVIGYANRPVRAGSWVREDVLDLPEPPAFADLPLATNVPPSL
                     PPLDGCTFDGYRNPDGSAGTKNILGITTTVQCVAPTVEYAVRRIKSEILPRYPGVDDV
                     VAITHTYGCGVAIDAPGAAVPIRTIRNLSRNPNLGGSPLVVGLGCEKLQPERLFPDAK
                     LQPTGAGLPVIGDDPWVLRLQDPDLRGFEAIVAAILRAAEKRLEELSRRRRKTCPASD
                     LVVGLQCGGSDAFSGVTCNPAVGYAADLLIRAGSTVMFSEVTEVRDAIHLLTPRCATE
                     AIARDLIREMKWYDEYLQRGSADRSANPTPGNKRGGLSNVVEKALGSIAKAGSSALVA
                     VAAPGERVTAKGLVFAATPASDFICGAQQLASGMNLHVFTTGQGSPYGLALAPVIKVS
                     SRTALADQWPDLIDVDAGRIATGRASIEDVGWEIFRLILDVASGRRQTWTEHWGLHNA
                     LAIFNPGPVT"
     gene            complement(31726..32292)
                     /locus_tag="VLE22_05730"
     CDS             complement(31726..32292)
                     /locus_tag="VLE22_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13938.1"
                     /translation="MMRAFVTFVLFAACLPAETPSEIARQLDEIGRVATVMVDGDAAK
                     RIVTKRALESFRKVDPKDPWVASDNFDVNHEPYIQVKKTLIRLSKLASFPCDVNLWSP
                     VEGMPGRIQIVIRNVHEMSQFWSWGVLHQDTPPRLKAVLETGKRVTVTDRPDMISVLA
                     PVYDSLGDVVAVAEAVAQEKPNPRENVK"
     gene            complement(32292..34109)
                     /locus_tag="VLE22_05735"
     CDS             complement(32292..34109)
                     /locus_tag="VLE22_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13939.1"
                     /translation="MRALIFLSCIFAFGLRAADLEAYSEFARLDPFGVVIEADRPAGQ
                     PLSGPNGVVALRGARGGYVSFRLAARLSKPGPYSVTFAWNTPATNLTAEAFREWFHYT
                     EPKNSYYPDALAPVKLPYRSQLPEPDNRIEKQTVQTFWVDIWIPRDTAPGVYRGEARI
                     EGDGKKVSRRIELQVLPASIPEKEIVAIDHNSYGSEWLSSQYPKLRQRLGEKFFASDE
                     FFGLIHSYHRIFYEHLGVFHQLGYGHGGKVGPEFAPELDGSGRTKRIKSWSLYDRHYG
                     PLLDGSAFAGTRRGAQPIPFVYLPINPEWPASFLWWGEPGYETEFVNVVREMEQHFRD
                     KGWTKTAFEMFFNHKKRYKAFPWDGDETRFPNDNRYFAEYARLLKKAVPPGSQVHFRF
                     RSDSSWMMGQQFKELAGVIDFWVLGGSELSWYKEVPAMLRARGDIVWMYGGTPEVTES
                     SGAITFAPFQTWIWGAEGFVRWLTVSPGADPWFRFGGGDTVLAYSGDRFAIDGPIPSV
                     RLKLQRNCVQDLTLLDSLKTRKPFEELRAGAASRYNSSRYEDWWPPRPAAADLPPYEM
                     TNATIAENSKPVKDLAARVDAQSWEKVRVWILQLAVGGK"
     gene            34143..34550
                     /locus_tag="VLE22_05740"
     CDS             34143..34550
                     /locus_tag="VLE22_05740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSB13940.1"
                     /translation="MRTHETSLRVRYAETDQMGVAYYSNYFVWMEVGRVEYCKATGFR
                     YRDMEEQDGVLLAVVGAECRYLSPARYDDEVIVKTWVEQANPRMIRFGYEMREASSGR
                     TLATGETKHLFCGRDMKPVKLPEKYWALIGIRS"
     gene            complement(34665..35258)
                     /locus_tag="VLE22_05745"
     CDS             complement(34665..35258)
                     /locus_tag="VLE22_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13941.1"
                     /translation="MTRSTLVRAAGLAAIAGGALRAAASFAPVVIGSDIERESLYIVV
                     DVCLTIGLVGFSARHSKSIGWPGAAGLALALVGLATVRANRAISTVDLYPAGALATAC
                     GVILLSARAWIARWIRGWVPVAFLLSTLIGLIGSYVRGANALFVWSGVIFGVAFAGLG
                     IEMWKSGSNSYPHDAASAGDSQSGERLLQPHSDQSQS"
     gene            complement(35583..>36391)
                     /locus_tag="VLE22_05750"
     CDS             complement(35583..>36391)
                     /locus_tag="VLE22_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HSB13942.1"
                     /translation="KRVSQGCVSVFLNDRATVGMTVEANGPFGHFCFDESKHQNVVLL
                     AAGSGITPMMAMLRYMDDLCLETTVTLLYCVRTSNDIIFHGELEELRARLKNFQYHLL
                     LSQPHTEWPGPRGHVSREFMENTLKEIALQDFFLCGPPPFMEASRAILIGLGVKPERI
                     RQESFGSSAPKSAQPVSAAAETDVVVEFARSGKAYAIRSDQTLLEAAEEHGVGISSSC
                     RQGQCGTCKTKLLAGNVRMDTQEGLDPDSRAQGFVLTCVGHADGAVKLDA"
BASE COUNT         6620 a        11603 c        11264 g         6904 t
ORIGIN      
        1 cacctggaca ccttccgcat catccggcaa caatgcagta cgccgctcgc gatgggagag
       61 gtgtacaccg gccagtggga cggcctgttc ctggtcaccg agcacctgat cgactacgta
      121 cggcacgacc tggttcactg cggcggcatc accgcgggca agaaggtcgc gaccatgtgc
      181 gaaccgtacg gcattctgac ggcgtggcac gggccgggca acatttcacc ggttgcgcac
      241 atggcgaact gccacgtcag ctactcggtt ccgaacttcg gcatccagga gtactcgacg
      301 ggttggagcc cggcgatccg cgaggtgttc acggcagttc ctgcgtatcg ggacggtcac
      361 gtcaccatcg gcgacgcgcc gggcctggga atcgacgtca acgaagcagc ggcaaagaag
      421 tacccctacc tgcgccggat gcgtccgacg atccgccgcg ccgacgggac cgcgtggcca
      481 tattgagcaa atcgtgccat aataacagct atatggcagg tatgcgtgat aatgccacaa
      541 ctgtcgattc tgggggaaga gtcgttctac ccgccaggtt ccggaagtcc ctcggcattc
      601 agcccggaga ccgagtgatc gttcggctgg aaggagatca tctaagcatt tacagcaggc
      661 agatggcgcg cagaagcgca caacagaaaa tctgcaagct tgtgccgcgg gacgtgtcgc
      721 ttgtgacgga gctactcgag gacaggaagc gggagtcgtc tcgtgagtag ttgcgttctt
      781 gatgcttcgg cggtccttgc ctacctgcac cgcgagcccg gtcacgaccg gattgagcga
      841 ctgatggaag gcggcgctgt cagcgccgtg aacgtgagtg aagtcgcctc gaagctcatc
      901 gaccgcggcg tgcgcgaaga aaccgtccgg gagatcgtgg gtgatctcgg tctctcggtg
      961 atccccttcg acgaagcgct agcctatcgc gcggccagcc tgcgtcaacc cacagaagcc
     1021 cttgggttgt ctctgggtga ccgttcctgc ctcgcgaccg cggcgcttct gggcgtgcgt
     1081 gctatcaccg ccgacagggc ttgggagaaa ctcaagctgc cggtaagagt gctggtggtg
     1141 cggtagggac ggccgccgcc gtttgcctac ttcgcctcca gcagcaggtg cttttcgaac
     1201 gcgacgtcga ctgacgcgac gggaccggtg accgtgccga ggtcctttcc gccgacgtag
     1261 tcggtaacgc gatacggccg gcgatcgagg ccgcgcagtt cgactttgcc tttccagttg
     1321 ccggcgaaga aggcgtagta catcacgccg tcttttcgga tcgcatgcgc ttcggggcga
     1381 tcgaagccga tgtcgtacag cgagccgaga tactcgccct tcgacagcat cttcgtcttg
     1441 tagatctcga gccagcgcgc ccagtgtttc tccctctcgg gcgtgagcca gaccttgcga
     1501 tctttaccca ccttgggagg ccaggtgaag ttcgttccga ccaccccgcc tattccgacg
     1561 gtggaggcga aatcgtcccc gccgtcgctc atctcgacgt ggtcgccgaa ataggtggcg
     1621 gagtctccaa gcagagcttt cagggtcttg cccttggtgc ggacctgcca ggaattgcca
     1681 ggatcggagg cgacggccat gttcatgtat ggcatggtga agaacgagta ggccgtgccg
     1741 cacgggcaga tctcgaccag ggcgtcggac tttacgccaa ccgccgcgtc gtagatggct
     1801 ttgaagaagc cgggcacacc ctcgacagcc tcttcgggcg ccgcgtgccg gtgggccggg
     1861 ttgtagcaag gcggggccgc attcaaatac tggccgtcga tcttcaggcc gtcaaagtcc
     1921 cagtcccgga gcgccttgac ggcgaatgcc gcggcgtcct ggcgaaccgg cggaaaagcg
     1981 ggacagaggt agaacgcatc ccaccaggag attttctgcc gcgagccatc cgcgttgagc
     2041 aggagttgct cagggcgggc cttgatcaga tcggtgccgg ggtcgacgga catcggcgcc
     2101 caccacaatt gcgccttgaa tccttcggcg tgaatccggt ccaccatctt gcgcacgtcg
     2161 cgatcccccg cggggaactt cttcctgttg acataccagt caccctcggc ggtctgccag
     2221 ccatcgtcga gcgtcaccca gcgaaagccg agctttttca cgacgggaag cgtgccgtag
     2281 acctgtttgg gagtgaagtt gcggccgtag ccccacgcgc accagatcgg ctcatacgca
     2341 tcggccggag gcacgggaaa ccgcaccccc tggctgatca tgaagcggcg gtactccgcg
     2401 agtgtttgga agtagtcgcc attgtgcacg gcgacgaacg tccggaaggt cttcagagtt
     2461 tcgcccggtt tcaaggtccg gttcgtcttg aagcgtacgg cgacggttgc gtgcccggcg
     2521 ccgggcatct cgaccggcag ggaaacctgt ttcggaacaa gctccacatg accgacaccg
     2581 attccggcgt cgcgtctcca gacatccgcc acaggtgtgc cgcctccgta gtcggtccca
     2641 ttcatgccca ggaaattctc ctggtggaag cccgccttca acggcagaac ccagtcggga
     2701 cggttctcgt aggagccgct ctggtaggac cagaacgccg gctcagcggc tcccggcggg
     2761 gcgtcaatta cgtagtgatg gttcgtccag ccaccgaccg taaggtcgcc gctcccggtg
     2821 ttggtatagc ggacttcgaa cagtgccatt tgtgggaagc ggtcggacac ggtcaccgat
     2881 acctcttttc ggacgcccgg cacttctccg gtaatgatgt agagtttttc tcctatccgc
     2941 tgctcgattc tgtcgccgga catccggaag tctctgatct ctttgccgcc ggcggtcagc
     3001 gtctcggaag ccgaaaacgg tccgatcgga acttccttga ggccgaactt cgcgatcacc
     3061 cggctgtgtg tggcctggtt gaactcgatt cggattccgg gcccgtcgac ccgagccgag
     3121 tccggcccgc ggaaggcgca accgaaaagc aacagtaacg aagaaagaga aacagcaggc
     3181 aacaagcgaa ggccacgacg cacgatcatc ctcctgatac cattctccac caggtcattc
     3241 cgggccgcga ccacggggac cggagggagc ggtcaggtgt gcccgccgtc ttattgacgg
     3301 cttcgagggg cgcggaggcg cgctctatgg cgtgccggcg tggcgcttga ggatctgcgg
     3361 gagattttga gaaaaggccg aacgggccat gacgaggtcc gctccgttct catgggcctt
     3421 ctgcttcaac tcgccgtgga cgtgcgaaac gaaggcgagc aggctggtcg ctttcaaatc
     3481 ggagttgccc ttcagtttgg agatcacctt cagcggctgg gcggctgaaa agttcaggtc
     3541 gaggatgatg acctccggtt tggattgcgc cttctcgatc aattctttgt cgtccttgac
     3601 gaactcgacg tccattccgg cccgcttggc ggcgtcactg atcttcaccg tgaagaacag
     3661 atcggacacc atcgccaaga ctcgcttgtg tacccgcttc tgcatttgcc tctgggtatg
     3721 caccttgggt tcgtgttgtt gcacaaaaaa tccaatcgct gttttgagtg ctactgctgg
     3781 aggggcaata caatcttact aattcttaac ccgcccgtgc aactgtagct gtcgctcaac
     3841 ggtgagcttc taaagggtct gatgacgcag ggttgcccgg cgttgcagga ttctgcaagc
     3901 gcggtgacgg cagagggtgc gtgttatgtt gagagtatgt ccgaggggaa cccgttccac
     3961 gaccctcttc gtttcgaacg tcacgcacca ccctgcgcgc tcgtcatctt cggcgcgaac
     4021 ggggatctca cgcggcggat gctcatgccg gcgctgtatc ggctggcgta cgaccggagc
     4081 ctgccgccgg gtttcaccgt agtaggcaac tcgcgctcgg caatgagcga cgagcggttc
     4141 cgtgatgtga tgcgggagtc tgtcatccag ttcctccggg atactccgtt cgatgagcgg
     4201 atatggcggg acttcgcgga gggtctgtat taccgggccg gcgatctgcg cgaccaagga
     4261 ttctaccgcg aactggagaa ggtcctggta tccgcggaga acgaacggca cagcggcggc
     4321 aacgtggtct tctatctggc tacgcagccc agcctgtacg aggcggcggc cgccggaatc
     4381 ggcgcggcag ggctccagca gtcggctgga tggcggcggt tggtcataga aaagccgttc
     4441 ggaagggatc tcgacagcgc ccgggacctg aatcggaagc tccatgcggt cttccccgag
     4501 gagagcatct accgcatcga tcactacctc ggcaaagaga ccgtgcagaa cgtgcttgcc
     4561 tttcgcttcg gcaacggcgt tttcgagccg ctctggaacc ggcgctacgt gaaccacgtg
     4621 cagatcacgg cggcggaatc catcggggtg gagggccggg cggcgtacta tcaggagtcg
     4681 ggcgcgctcc gcgacatgat ccagaaccac ctgctccagg tgatggcgac ggtagccatg
     4741 gagccgtgcg cggtgtatga accgaatgcg gtccgggacg agcgggcgaa actgctccgc
     4801 tccatacgga ttctccgaga ggacgaagtc gacagccatg ccgtcgccgg acagtacggg
     4861 ccggcggaca tcggcggcga acaagcccgc ggtttccgcc aggaggacgg cgcgaatccc
     4921 gattcgcaga cggacaccta tgcggcgcta gcgcttttcg tcgaaaactg gcgctgggcg
     4981 ggtgtgccgt tctatattcg gaccggaaaa cgcatgccga ggcgggtgac cgatatcgcc
     5041 atctccttca acaccgctcc gttttctctg ttcggttccg acggccagac gggcctcatg
     5101 gcgacgacgc cgaatcttct ggtcctgcgc atccagccgg aagagggcat ctcgctgcga
     5161 ttcctgtcga agcagcctgg aggcggcatg aggcttcgcc ctgtttcgat ggatttcaat
     5221 tatggctcga gtttcggcgc gcggctgcca tccgcctacg agacactgct gctcgatgtg
     5281 atgactggcg atgccactct ttacaccagg caggacatgg tcgaggcgag ctgggcggtc
     5341 gtgcagccgg ttctgaacgc gtgggccgga cggcgcttcg actttccgaa ttacgctgcc
     5401 ggaacgtggg gcccggcggc tgccgacgag atgcttgcgt gtcatggtca cgcctggagg
     5461 aagccctgat ggccgccgtc gtcaatcccg agcggttact tcgggaactc cacggcctat
     5521 ggatcgacct tggacgcgag gccgaagcgg atggtgggcc tggagtcata cgcgcctgcg
     5581 cgctgacgct catcgtcatg acggaagacg cggccggcgt tgccgaaaca ctggccgagg
     5641 tgatgcgcga acatccgtgc cgcggcatcg tggtatcggt tcgcccggac gcctcgtctg
     5701 tggacgcacg ggtatcggct cagtgctggc ttccgctagg gcagaaacgg cagatctgct
     5761 cggaacaaat cgagattacc accagcggcc caacactcgg gcacgtatac cctctgctga
     5821 atgcgctgac ggcgcccgat ctgccggttg tggtgtggta ccgcggcgcg agtttattcc
     5881 ggtcttctga gttgcgatta cccctgtcga tggccggccg tctgattctg gactccgggc
     5941 agatcgaggc gccgggcgca ttcctgagag aacttatcga ggtcccccat aatgggctcc
     6001 gcgccggaga tctcgcttgg actcggctga cgtgctggcg ggataagatc gcccagttgt
     6061 ttgaggccca tgtctgccgc gatggaagca cggggatgtc ggaggttcac attttgtggg
     6121 aggggcggac ggcgcgctcc gatgttgtgt acatggccgc ctggattgct gaagcactcg
     6181 gctgggagcc gcaggattcc cggctctgcg tcgaacgcgc ccgcggcgcc ggagacgggc
     6241 gattgctcgg agtacggctg gcggcccccg gtatgacggt cgcgatcgcg cgagaggatg
     6301 gcggcaaagt ggccgtcacg ctgaacggca tgctccacga agcgttcttc cccgagcgaa
     6361 gcgaagcttc ggcgcttacc gaagaactgt cgattgtgga acgcgatgcc gtctacgagc
     6421 acgtgctgcc gttggcggcg cgcctggtcc ggcccggatt ggagtgatct gtggacgccc
     6481 ggcgacactg ctttccgacc ccgcaactgg ccgccgaggg gtgtgcccgc cacgttggat
     6541 cgctcatcga ggaaactttt gccgagcagt cgcgggccac tcttgccgtc tccggtggaa
     6601 atacgcccaa actcctgttc gaacacctcg cggtctcgtc cctgccgtgg gatcgggtgc
     6661 acgtcttttg ggtggacgag cgggcggtgt cgcccgcgga cccgcggagc aactacaggc
     6721 tggcggagga atctctgatt catccggccc gcataccgga gcgcaacgtc caccgaatcc
     6781 acggcgagtg gacgccggat ctcgctgccc gccggtacgc gggcgaattg cgggagtttt
     6841 tcggactttc cgagggcgag atgcccgtgt tcgacgtcat cttgtgtggc atgggcagtg
     6901 acgctcacac ggcaagtctt tttcctggag aacctgcgat tctggaccgc gagcgcatcg
     6961 cggctgcggt ccgggtcacg aaactgccgc agtcacgcat caccctgctg cccggtccgc
     7021 ttctggcggc tcgccatctg gtcttcctcg tgaccggcgc ggacaaggcg gaagcggtac
     7081 gcgcggtctt tcaggagccg ccggatccgc tgaagcgccc ggcccaactg gtccgcgacc
     7141 gcaggacaac ttggttcttg gacgacgcgg ccgccagcct cctcgagtga agtgaaatga
     7201 cgacccggcg taactttgtg cttctccccg ccgcgacgct cttgtgcggg cgccgtccgc
     7261 cgtccgggga cccccggccc gagccgttgc ccgcgacgcg gctggtgttc ggcggcgacg
     7321 tgatgctgtc ccggttcgtg gggaggctgg ctcgttcgca gcgtgatcct acttggccgt
     7381 ttcaggcgct ggcgccgtac ctggcgggcg ccgatatagc cttcctcaat ctcgaatcgc
     7441 cgttcagcga ccgcggctgg ccggtcgaga agggcatggt gttcaaggcc gaaccggaga
     7501 tgatcgatgg cttgctgatg gcgggagtgg acgtcgtgtc gacggccaac aaccatgccc
     7561 gcgactgcgg ggcctacggt atcgagttca cgctgggctg gctggcgcgg cacggtgttg
     7621 cggccgccgg caccgggatg accgaggagg aagcccgcca gggtgcggtg ctcgagagaa
     7681 acggcgttcg cttcggtttt ctcgcctaca cgtatgatca gtccaacgga aatcatccca
     7741 agcccgatcc ccgcgtcgcc atgttggacc tggagcgaat gagggaggac attgccggga
     7801 tgaaagcccg gtccgatgtg gtgatcgttt ccatgcacgc cggcgttgaa taccggccga
     7861 aaccgaacga gcaacagctt cgctttgccc gcgccgccat tgatgccggc gcccggctgg
     7921 tcgtcggcca tcaccctcac gtcgtgcagc cggtagagaa atacggcgag ggagtgatct
     7981 tctactcgct cgggaatctg atcttcgacc agttccatcg ccgggaaacg cagcgcgggc
     8041 tcctttcgga ggtcactttt cgcggtaagt ggctggagcg ggtggaatta cgggatgtcg
     8101 agataccccg cggggcgccg caattgtccg gcctgccgtc cgttttcctg gagcggtcta
     8161 ttgctccagc cggcgggtga gcaccgaggt tccggacacc agtcccattt ggcggccgag
     8221 ttccaaggca gtccagttca cttcgaggga gtggtccgag acgagcctga gccggacctc
     8281 gcctcgcgct ccccccacac ccatccagtt aaggtgggcg gcgtcccgtt ccgcttcgcc
     8341 ttcgaactgg aaccgcactt ccgaaggaat cgccctgtcg gtgatgacat atcgtgcgcg
     8401 ataacggccg cggatcgatc cgttgtgctc cgaaatcacc atctcaatat agtccggtgg
     8461 gtaaagcgcc atggaatcgc tggttagggg tagtggaacg tagacccagg tccccgtcca
     8521 ccgtccgtgg atttcgtcgg gcgggggaat gcccgagatc ggtggagaag cgctggcgag
     8581 agatcgtcct gcgggcggcg catgggctgg tggcgccgat gtgtcttcgc cggccctccg
     8641 gaactcctcc cgtgcctgca aatcatgctc ggcaagctgc gccaacgccg tgtcccgctc
     8701 ggaccgggcg gcctccagtt cccggcgcag tgaaatcagt tcggacgaca aggccgccac
     8761 ctgacgccgg cgagccgtgt ccggcatcac ggcgcgtggg ggcatcgcac ttgctttcgg
     8821 gtcggcgctg gttttcccga ccgctgaagc cggttgcggg ggcggggaag cctgtgtgcc
     8881 gacgtacccg cacatcgcag cgccggcgat cagcgcggtg accacccaga ttgcgtcacc
     8941 tgcggccagg ccgcgccgcc ggcgaggaaa ggcacacgcc ggacggaggg ccacaagacc
     9001 gggatgcagc gcgcctccgc tttccccgtc ccgcagactc aagtcgtact tccgccgctc
     9061 gaccgggtca cccagcgtct cgtgaatccg gttgagccgc ttcatctgga cttcggccag
     9121 ccgccggacg ctctcgtcgg gttgttggtc cggatgcaga agacgggcca ggttccggta
     9181 ggcgcggtga atctcgtccg ccgaggcatt ctcagccagc ccgagttctt cgtagtaatt
     9241 cactgcgcgc gatgcacctc cgggtaaccc atcggctgaa ccgccggttt aaagtaggtc
     9301 tgatagctta caaccttatg cagttagcga cccgcgacca aacggagcgg tcatgaggaa
     9361 ccgcgacagt gagggagcgc aagcaagccg ggttcttccg tacctggagg ggcggcgtgg
     9421 cgggcagacg gccccgcgat tattctccag tacgaggata gaggactggc tcgctcgtgc
     9481 cccgatcgtc gttactgaag gacccgagcg cggcggtgtg gttggctggc tggatgaaga
     9541 cgatgtggcg cctttcgttt atggtgaggc gagcggctac tacctgacgt tcttgacgtg
     9601 gctggcccgc gaaacgggca tttcgttcac gacatgggcg cggagggagg cagtgctcgg
     9661 ttggtgccgc cgctggacgc aagggaatcg cgtgccgccc acccgctgta tcttggacgg
     9721 gtacgcggct gactggcgca acggtttcca gttctccttc gatctggcta tggtgctgcg
     9781 cggtctggtc cacgccgggt gcgagtcccc ggcccggtcc ctgttggcgt acctgtgtcg
     9841 ttttgtatct ccggccggca ccctgcgggt cgctctccct ctcaacaaag actgcgcaga
     9901 cgtccatcac tggtccgttg aacccggacc gtgtcagttg aaaaccgcgg cggctatcct
     9961 ctccgcctcg gacttcggca tccccgaggc actgcgtgtc gccgtgggca ggagtatcga
    10021 ttgttggcgg cgcacgacgg atccgtccgg ggaggctcat cctttccttt acttcatcga
    10081 gggattaatt ctctgcggtt actacttggg tgatcgatcg ctttggagga cggcggccga
    10141 aacttacgcg cgactgctgg accaccagac ggccgactgc gggctccccg cctcggttcc
    10201 ggattcgggt tccgttcaag gatccgatgt catcgcccaa gctctgcgga tcggctgcct
    10261 gctgcgccac ctcggacacc tgaagccgta tttcgacacg agactcgagg gccttgcttc
    10321 agccctggat cgtttcttgc tcgcagatgg cgcggtgagc tttctcgatc cccggctggc
    10381 cgcccgcacc caccgtaaca cgtggtgtgc aatgttcgcg caccaggctt actgtctgta
    10441 tggacggttt cttcgcggag cagccatgcc cgcgagtttc ctggatgtga tggtctgacg
    10501 atggagcccc ccgaagtccc gccagccgac gagataccct gcgatcctcc gcgcgtgctt
    10561 tggattgagc tgacgtcccg ctgccccttc gagtgtatct tttgctcgcg caagacgcta
    10621 cgaggcgcgg gaagacacat ggatttcggg ctctgccgcc ggattctgga tcaacttcgc
    10681 aatccggaag tcatccgact gaactattcc ggggaatcga ttcattaccc gcggctggcg
    10741 gaagcgatcg agctggcgca cgcgacgggc gcgcgaaccg agttggtgtc ggcgcttgca
    10801 tcggcgcccg agcacctgct cgaagcgctg gtcacccgcg gactcgaccg gctgaccgta
    10861 tcgcttcaca ccaccgcctc caatcagttt gaagagatat atcgctatag ctctctcgac
    10921 aacctgcgag cgaagctggc gctcctgctt cgtctgcgag aagagcgcag aacggcaaaa
    10981 ccctcgttgg atttcgcctt cgtggcgatg cgccgaaacc tcgatcaact gctcccgatt
    11041 gccgagttcg cccgggagtg tggagtaagg gaaatccgcg tctttcctgt catccgtcga
    11101 gacgagatcc ctcatcgttt cgatcaggaa ttggaggatg ggcgcttgcg cacctccttt
    11161 caacgggaaa tccttgaaac cgtcgagcgg gtgcggcaac tctgcccgga cgtccaattc
    11221 ggcgtgacgc ccgccgaaac actcgatgag ccggcgctcg gcccccaacc ccagccatac
    11281 tccggggcgt tgccaccggg aggcaggatc tactcttgcg agcagaaccc ttgggagacc
    11341 gcccacattc tggcaaacgg tgacgtggtg gtgtgtgagg tgcaggacaa agtgagcatg
    11401 gggaacctca acgacgttcc cctcgagcag atttggcacg gcccgaaata cagcgcgttt
    11461 cggcgccgct acgccgatgg cgaagatccg gtgtgttgcc gctgtcccta caagattgcc
    11521 tacgccccgt cggcaatgcg ctgctgcgtg gaagcggggg acgggatgag ctttcagctc
    11581 gcccgcggct ggcacttgcc tgcgcacgaa gacgccatct ggagcaagtg gcattgttgc
    11641 atggtattga aaggcaatgg gttcggcgcc aagcttcaga ttcggggcat tttgcccccg
    11701 gcccgggacg cctcgtccaa caccctttcg ctgcgagccg gaggccgctt cctcggttct
    11761 atccgcaacc cgtcgggtga catgctggcc ttcgattcca cattcacctt gcctggcgat
    11821 gccggcacgg taacgatcga gtgttcgaca agctcggtct gccgtccggc ggagcaaggc
    11881 gccagccgcg acgagcgcga gttaggcttt gccttgatac gagtggccgt cgagaagaag
    11941 gaatctcgct ggctgcggtg ggcgttgtac gcagccgact tcctagtccc cttcgcgaga
    12001 cgcctgcgcc cctttcgcag acgtctgtcc gccgcgtgga gtcccggaat cagcatcgtc
    12061 atccccgaac gcggctcgcc ggaaatgctt cgtgaatgtc tgtccggcgt tcgggcggcg
    12121 gcgggccaag cgcttgaacc ggtcgagatc ctcgttgtgg tcaacggtgc gccgcttttg
    12181 cagtaccggg aagttgcgcg agagtttccg gaaaccattt ggctcaccag cccgcggccc
    12241 ttgagttttg cccaggcggt gcggcgggga gtgcggcgcg ccaggtacga ctgggtcttc
    12301 ctcctcaaca gcgatgccgt gcccgaaccg gacgcgctgg cacaactgtt gccatggcgc
    12361 ggaccgcatg tttttgcggt cgcctcgcgg atcgttctcc ccggcgcttc ggcgcacccg
    12421 gaggagacgg gctggacaga tttccggcaa gtgcgcggcg aggtcgaaat cttcgacgtg
    12481 gcgccggagg acgaggagac tgtgcggggc tctctctacg ccggcggtgg cgcctccttg
    12541 ttccggaaag cgctgctgag gcgctttctg gcccgcagag atccgtacgc tccgttctac
    12601 tgggaagatg tcgagtgggg cgtgcgcgcg tggcgcgccg gctacgaggt gctgttcgcg
    12661 ccgaaatcgc gggtccgtca ccgccgtaga gcgacggtct cgcgctacta caaggaccac
    12721 gaaattgaca ggattttccg gcgcaaccag attcagttta tgctcaggaa ccgcttcgat
    12781 gacggtgacg cgcaccggtt gctcgccgga ctggtaaagg aagatcccac cacacaagcg
    12841 gcgatcctga agcccgggaa ctgcctgcga cttctgtggg cgcgcgttag gacgagcgcc
    12901 gctcgaaatg cgaacttctg cttcccctac gtccgcagga agttttatcc caccccgttc
    12961 cagtccctgc cggaaggtcg cgcgatcctg gtggcctccc cgtatgcagt cttcccgccg
    13021 gcccacggtg gcgctgtccg cctggcacgc ctgctcgaac acttgaaacg ccagcgcaag
    13081 atcgtgctgt tgagtgacga agaatcctgc tatcaggaac aggccttacc ttacttcgcc
    13141 ggcctggcgg ccgtgcacct ggttgggggc aggcgtgaga tgtgcgagca gcgggcggac
    13201 cggatcggcc gcatcctcag ccattcccat gacatcctga agcgcgaggc cacccggctg
    13261 gcggaaagtt actctgttgg cgccgtccag gtggagttcg tcgagttagc caagctggtc
    13321 gaggcccgcg aaggcgctgc tccttggttt ctcacgcttc acgaagtgct gctggagtca
    13381 ggcggccgcc ccgaagaaga tagcttcgag aaagaatgga tcgcccgcta cgatgccgca
    13441 atcgtctgct cgccggagga tgcggcgttg ctccaccacc gggaggtatt ggtcgcgccg
    13501 aacggcgtcg agatgagagg cgatgtgtac gtgccgtccc gtggacgccg aacaatcgcg
    13561 ttcctcggcc cgtttcgcta ccggccaaat tgggatggca tccagcgctt ccttacgtgt
    13621 gtttatcccc gactccttcg cacgctcccc ggcgtgggca tcgatgtctt tggcggcctt
    13681 ggagcgctgg agcgggccgc cggggtcgac tgctttcgcc agcccggtgt gcgggtgcat
    13741 gatcatgtcg aagacgtacg ccggcggctc gatgcctgcg ccgtcaccat caacccgctc
    13801 gatcaaacac gcggatcttg tctgaaggtg atcgagtctc tggccgcggg ccgtgtctgc
    13861 gtatccacag tcgcgggtgc gcgtggtttc cttcgggacg gcttccgctc gctggtcgcg
    13921 gttccccgcg ttggggactt tctcgagccg cttggtctct tgctctccga ggaagaccgc
    13981 cgcctggagc tcgagccgcc ccaggcagaa ctcttagacc gctaccgttg ggagcatgcg
    14041 gcacgcaaac agctcgattt gtaccgccgt tacagagagg cgtcgcaaca agccccaatg
    14101 tggcgtagcc tatacaggtt gttgaaaaac ccgctggacg ggtcggcaac ctatccggcg
    14161 agtggcgcag tcgttgaccg gaagctgtga aaaagcctca gacgcgcagg acgatctttc
    14221 cgaactgctc tttgccctcg aggcgccggt gggcttcggc gatctccgaa agcgggtaga
    14281 tgctgtcgat aactgcgtgc acctgcttgc ggaagacgaa ctgaagaacc tggtgcagct
    14341 ctccgagcga ccccatgaac gagccgagga gcgactgctg gcggttaaac agaaaccgca
    14401 gatcgacctt cacgtcgaag ccggtagtcg ctccgcaggt caccaggcgg cccgccggag
    14461 ccaaagcctg cgtgctcttt gaccaggtgg cggccccgac gtgttcgaac accacctcga
    14521 ccccgcggcg gccggtcagg cgcttgacct cggcggcgac gtcctgccgg tagtggtcga
    14581 tcacctcgtc ggccccgaga tccttcgcct ttgccagttt cgcctcgctg cctgccgtgg
    14641 cgatcacgcg gcagcggaac agctttgcaa tctggatggc ggcggaaccg acgccactcg
    14701 acgccgcaag gaccaggacc tcttcgcccg gctgaagctt cgcccggtcg aagaacatgt
    14761 gccacgccgt cagaaagaca agcgggactg ccgctgcctc ctcgaagctc aggtcgtccg
    14821 ggatggggat cgccgtgtac tcgggcgccg ccatcaattc ggcgttaccg ccgtcgacgc
    14881 cgtagccgaa cattgtgtag cggcggcagt ggttgtcccg gcccgaaacg cactgcggac
    14941 actgccggca actcaatccg ggggcgagca gcacgcgctg gccggtccgg atgcgctcgc
    15001 aatactcacc cacagcgatg acctcgccgg cgatgtcgct gcccagcacg tgtggcatgg
    15061 agaactccat gccggggatt cctgcccgga cccaaaggtc gacgtggttc agcgcacatg
    15121 ccttgactcg cacgagaacc tggtcagccc ggatctgagg ctcgggcgcc tcctcgtacc
    15181 tcagaacctc cggtccgccg tgctggtgga tacgcgctac cttcatgaat gccgtccctt
    15241 tcctccacat tgtccttcaa cttccagcgg agggttagga acgtgaattc ctcaggcagc
    15301 ccggcgccgc aagcgctgcc cgagcaagat gctgagcaac ccggcgcccg cgagaatcag
    15361 gctgtcggct tccggcgtct cttgcatcgc cgcaccatag gaaaagttat cgatcatcgg
    15421 agcgccattg acgggcgcca ggcgaacaaa ggtgatcggc acgtcagagg tgaatccaac
    15481 aaacgcggga ttcgggttgc tgaaggtgtt gactgtgcgc acgtcgccgg tcgaaagcgt
    15541 caccacaatc tgcgccgcga acggcgtgtg ggtcatgatg ttcgtgccga acgatgtaac
    15601 ccctccagac ggcatgttca cctggatgta gccgcctccc caggcggcga gtggtcccat
    15661 cagcacatcg cctgaacccc aggtgtaatt ctgggaactg gcgggatcct gtgcgtacag
    15721 atagtaactg gccggcggtg tgaggccgat gaattggaga cctcctatcg tgtaaccgcc
    15781 ggcggtgctg tagttcccag ggaccgcggc cgtggccagt ccctcaaagt caacggtgaa
    15841 aatgcccgaa gtcgcggcct cccacgtaat tctgtcggtg tagagcacgc atgttgcgcc
    15901 tgccggagcg gccaggaacg cgcaaacgat caggacgatc aggataagac tcagtctgct
    15961 ctctcgcggc atgaagtggt cctattcact taggtgggcg gacggagtgg attctctcgg
    16021 accgatctga gaatttctag cagtcggcgg gagtattccc cggtgtagtc cggaaagatg
    16081 cgggatcggg gctacccgag caccggcgcc ggagggacga tgcagatccc gtgccgctcg
    16141 atgtgttcgc tgtgctgcgg catctcgggc attcgcgcgg caacaggcag cgagacctgg
    16201 cgggcgcgcg tatcgaagtc cgcccatgac cacaggccaa tccggcaacg ccgatccgcg
    16261 gccattccgt cgaactttat gcgcgaggac ccggctccga tttcgatggt gtaccacgtc
    16321 catacgctct cgtgcttgag tacatccttc caatacgagt ccggagacgc ggagtattgc
    16381 ccgatgtgtc cctccgacga agtcacaccc ggttccaccg gcttcccgtt caccgtgatg
    16441 cggcacgtgc tggcatgatg cacgcggccg gggaattccg ccaggaagaa cattttcgcg
    16501 ccggaagctc cggtggggac ggagacatca cactccagct cgaagccggc ggttggaacc
    16561 ggcttgtcct tatccttcag ccattcaccc tcagggatgc ttcggacagt tctggaaatc
    16621 aggctgccac ggaaatctcc gcgcgtgctc gcagtggtct ttgtgacgga gtcccggcca
    16681 cccggttcca gcacgcgcac gctctctacc tcaaagtcgg gagaggcaaa cgcttgcccg
    16741 cctgcatccg cgtaccaacg cccgccaact atgaccggtt cccgaagttg ggcgcgcggc
    16801 acgacttcga cgaacaggag ctcccacgga gccagacgga gcgtgatggt tgaggcggac
    16861 gtgacgccgg ccgcgatgcc cttgcggtaa gggtactgcg tatagcacac cgcgtccgtg
    16921 agctcgcggg gggcgccgga acgttcgaga tcgaggatgt attccctcgt ctcattgctc
    16981 gggttgcgca ccgcgatgac gccgcgcgcg cctagccagt gcgcgtacgc atacggttcg
    17041 cccagttcga cccgagacgg caggaccacg gtgttcctga gaaggtccgt gttcttgcgc
    17101 gcccagcgaa gaagcccggc gtagatccgc cagtcgtcgg cggtcatgta cttcgggttg
    17161 acgtacgtcg gcacgaaaaa ccgtccgcgg ccgaaagcca tggcggcgtg gttggcgaag
    17221 ccggcggagt catcgcagtg cacgatgtcg aagtactgga gcgcgttttg cggcagccac
    17281 acctcgttca atgacgagaa gatgtagtac tcccgagcgg tagtatgggc ctcacgataa
    17341 tctggcgcgg gaccgaggcc gggcgggcag tcgccgccgg cattccccca gatgacgtcc
    17401 gcgtgcttga cgatccacgg gctgatgtaa tttacgtgag aattcatgca cgtcggctcg
    17461 gcaaccagat tcgggttggc tgccttggac gcttcgatca gctccagcat gtgcgaggcc
    17521 agcggctcca ccgagtcgtc gccgggcagc aggtcgtggt ggccctgctt ctcgaaggcg
    17581 atgaacccgt cgtacttgac gtgttcgaga cggctgtcct cgatgagttt cttcaggtgc
    17641 gttttcgttt tctcgcgata cgccgggtcg gccagcgaga ttccgggccg ccgctggttc
    17701 gggcgcatgt ggtattccag cacaacgtga ccctggctct ttgcccagtc gtagtcggtg
    17761 ctgttcgggt aaaccgcgct gggagacatc cagaggccca tcttccctcc tgccgattcg
    17821 accacctcgc ggacgcggtc gaatccgttg gggagcttgc gccggtccat ctcccagatg
    17881 ctctgtggga gagcccatcc ctcatcggtc gcgacgacgt cgaagaacag cccgtgagcg
    17941 tcacgaagtt gtcgtttcag ttcctcggcc agcgcctggt agcgctccgc gctgaggtct
    18001 ttggcataga aggtccacca ggtgttgtag caggccacaa ggcgcggcgg agcgactcgg
    18061 atgtcatcga tgtaccgaag aaaagcctcg cgtacgcgcc gcggcgcagc acagcccgcc
    18121 acggtagagc gcaacgggcg ccaatccgag ccgatttgca tcccgccagg ccggctgcgc
    18181 agcacgaaac cgtccggacc atattcattg aaggcagcga caaactccac tccggcccag
    18241 aggctgtcgc aaaagatcgg gtggctgccg aacggccggc ggtcggcatg gagggactgc
    18301 cagtcccgcc tgacgccttg ccagttgtcg aggtccgcgc ggagaagccg cgcaaccgga
    18361 ccgctcccgc gcctcagcga aatctgtttg cggaggtacg cctttcccgc cggcagatga
    18421 tattcgacct ttgcctcgag tcctgccatt gcccctgacg tcctggaaaa cacgagctcg
    18481 atgcggtcag ccgccgcgcg cgtgacctta cccgagagat ccgcgcagtc cacggtctcg
    18541 ccaccgtcga actcgaacag aaagtcgcgg cgggggagtt gcgctgcctc gttggacagc
    18601 ttgtttgtga agctgcggct tacgagcacg ccgtcaccga ggccgacact gaacttcagg
    18661 gcctcgttct caatcgtgtg ttccttcgct gccccgcgca gaacgagcgg ggttgctccg
    18721 aggatcaggt tctttgcgca ctctcgccgt gtgagttgac tcgatggcat ccggattctc
    18781 tccctgagga acagccaaga taccaccgat caggtcaatc ccgccgaagc aagcggagtt
    18841 ggaccaagcc gtcggagccg ctcgggatgg taaatgagag aagcggctcc gatggagtct
    18901 gccgggttta taatcgctgg tgacccgagg cgaaagtggg aatcctgggt gaagtactcg
    18961 ctctgacgct acttggcgta tcggccgtcg aagcgcagca gtacgacatc tcgactgtcg
    19021 tcggaggttc cccccctccg acgccgatag cgggagtgca tgtctccatt ggatttccac
    19081 taggtacggc cacagatact gccggaaacc tctacttcac cgcactgaac tgcgtgttta
    19141 ggctcgattc gaacggagtg atgacgcatg tggctggcaa ctcacgcgct ggctactctg
    19201 gcgacggcgg cccggccacc agcgcgcagc tcgattccac aagcaaaggt ttcgactggc
    19261 ccgtccctgc aggcgtagca gtggacgcta gcggcaacct ttacatcgcg gatagcagca
    19321 accaccgggt ccgtaaggta tccgccgcgg ggatcattag cacggttgcc ggcgacggtt
    19381 cccctggcta ctccggcgac aacggcccgg ccatcagcgc gcagctcggt gtcccctctg
    19441 gcgtggcagt agacgccggc ggcaacctct acatcggcga cactggcaac tatcgcattc
    19501 ggaaggtatc ggccgcaggg atcatcagca cggttgccgg cgacggttcc cctggctact
    19561 ccggcgacaa cggtccggcc atcagcgcac agatatctcg gccctatggc gtggcagtgg
    19621 acgctggcgg caacctctac attgctgatg cagacaataa ccgcatccgg aagatatccg
    19681 ttgcgggaat catcacgacg gtcgccggca atggttccgc tggctactcc ggcgatggcg
    19741 gcccggccat cagcgcgcag cttgattacc cttggggcgt ggcactggac gccagtggca
    19801 atctgtacat cgcggaccta cgcaatagcc gcatccggaa ggtgtccgtc gcccgagtca
    19861 tcaccacggt tgccggcaac ggctccgccg gctattccgg tgacggcggc ccggccgtca
    19921 acgcgcagtt ctttaatcta tatgccgtgg cagtggatgc cagcgggaac ctccacattg
    19981 cagacacggg caacagccgc atccggaagg tgtccgccgc gggaatagtc acgacgactg
    20041 ccggcaacgg ctccttaagc tactccggcg acggcggccc ggccgccagt gcgcaacttt
    20101 ctcaacctcg gggcgtggca gtggacgctg gcgggaacct ctacattgca gacacggaca
    20161 acttccgcgt tcggaaggtg tccgccacgg gaatcatcac cacggttgcc ggcaacggct
    20221 ccaggggcta ctccggcgac ggtggtccag ccaccagcgc gcagctcctt aacctctatg
    20281 ctgtggcagc gggcgccagc gggaacctct atctcgccgg aattccccat atacggaaag
    20341 tatctgccgc agggatcatc actacatttg tcggcggcga ttcccctggc tactccggcg
    20401 acggcggtcc ggccgccaac gcgcagctta atttcccaaa cgacatcaca gtggacgccg
    20461 gcgggaacct ctacattgca gaccaaggca accgccgcat ccgcaaggta ttcactacgg
    20521 gaatcatcac cacggttgcc ggcaacggtt ccgctggcta ctccggcgac ggcggcccgg
    20581 ccgccagtgc gcaactttct caacctcggg gcgtggcagt ggacgctggc gggaacctct
    20641 acattgcaga cacggacaac ttccgcattc ggaaggtatc caccgcggga atcatcacca
    20701 cggttaccgg caacggttcc gctggctact ccggcgacgg tggtccgggc gtcgacgcgc
    20761 agcttaatta cccgaacggc gtggcagtgg acgctggcgg gaacctctac attgcagacg
    20821 agggcaacaa ccgaatccgc aaggtatccg ccactggaat catcaccacg gtcgccggta
    20881 acggttccag gggctactcc ggcgacggtg gtccggccac cagcgcgcag atcacattcc
    20941 catctgacgt ggcagtggac gccggcggga acctctacat caccgactac tccaacaacg
    21001 tcatccgtct cctcacgcca acctcttcca cttgcaccta caccctctct gcgacgtcgc
    21061 tccaatctac ggttttggga ggaagttcca cgctgaccat caccaccgaa tccggctgcg
    21121 gctgggccat ctccaacctg ccggactgga tcaccatctc cggttcggcc accggttccg
    21181 gctctgcctc gatcaccctg gtggtggccc ccaacttctt cgcaccccgc gccgcacaga
    21241 tctttgtagc cggtattacg gtgacggtct accaggcctc gaacgtgctc tggataagtc
    21301 cgggcggggt ggtcagcgct gccagctaca cggcgcccgt cgctgtcggc agcatcgctg
    21361 ccatcttcgg caacttcctt ctccccgagc cggcctccgt ttcctcgttc cccatcccga
    21421 cggatcttgg ggggctctcg ctgctgttta gcggtgcacg cctggctccg ctgttctacg
    21481 ccaacgtggg gcaggtgaac gcccaggttc cctgggaact ggccggcctg ccgcagacca
    21541 ccatcaccgc cgccctcgat ggccagacaa gcgtgcccca gacggtgact ctggcaactt
    21601 tcgcgccggg catttttgcc accaacagcg gaggcaccgg gcaaggcgcg gtcctggacg
    21661 aaaactacca cctcgtcgat cccaccaacc cagcgactgc aggcagcacg gtggtacaga
    21721 tttattgcac cggcttggga ccggtgacca atcggcccgc gaccggtggg ccggcaccca
    21781 ggaaccctct ggcggagacg accacgcaac ccgaggtgac gatcggcggg gctcgggccc
    21841 aagtgtggtt ctccgggttg gcgccgggct atgccggttt gtaccaggta aacgcacagg
    21901 tgccgcaagg cgcagcgaag ggccacgcgg tgccggtagt gatctccatt ggcggggtgc
    21961 aatccaacac ggtcacgatc gcggtgcagt agcttaacaa tcgaagagag cgctagtgaa
    22021 gtccgccggg ttccagaaat tgggccggtt ggacggctga caacctgagg acagttaagc
    22081 gcagtgattg aagtaaaggc ggcgaaaaag cggccgctcc gagcctatcg cagcgaccgg
    22141 cgtacctttt cggcgtactc ccgggccttg gccagcaccc gtgcttcgtc gagcgtcaac
    22201 atgcggcggg cgcggacgat catgcggccg ttcaccatca cgtgctcgac gtctgatccc
    22261 ttgagggcgt aaacgagttg cgagtacacg tcgtacagcg gcactgcatg cggcctgtcg
    22321 aggcgcaggg cgatcaagtc cgcccgcttg ccggcttcga gcgatccgat ctccttttcc
    22381 aggccaagca ctcttgcgcc ggtaatcgtc gccatttcga atgcctggcg cgccggtagc
    22441 gcacgcgggt cgccggatat gaccttctgg agtttcgagg cgaggtccat ttcctcgaag
    22501 agattgaagt cgttgttgct gccggccggc ccatcggttc ccagaccgac ggcgattccc
    22561 attgacagca tcttcacgac gggcgccacg ccgctcgcca gtttcatgtt gctcgacggg
    22621 cagtgcgcga tgcccacgcc gcgcttcttc agaattccga ggtcggcatc gtccacccac
    22681 acgctgtggg cgcagatcgt gcggccgttg aataccccga gagaatcgag gacgcgaacc
    22741 gggctcatgc ctcgtttggt gactgcgtca tcgcgctcct gcttcgtttc agacaggtgc
    22801 gtgagcagcg ggacgccgta gcgattggca agcacgcggg ccgccttcag cgtctcgtcc
    22861 ttgttcgtga aaagcgcgtg cggcgccggc gccgggacga tcagcgagtc gtccttgaat
    22921 cgccggatga accgttccgt gaaagcgagc gcgtcttcgg gcgttttcgc atccggagcg
    22981 gggaagtcga ggatcgtctc acccaatacc ccgcgcaggc cggcctcctt cgtcgcttcg
    23041 gcgatcacgt cctcgaagta gtacatgtcg gcaaaggtcg tggtgcccga aagggccatc
    23101 tcgagacagg ccagtcgggt gccccatcgc acgaaatcag gggaaaggtt ctttgcttcc
    23161 gcgggaaaga tatacttctc cagccattcc tggaggcgca gatcgtcggc gatgccgcgg
    23221 aacagcgaca tcggagcgtg cgtgtgggta ttcaccaggc ctggcgcaat caggccctgc
    23281 ggcaggtcga tccggcttga ggcccggaac cgccgttcga tgtcggtgcg cgtccccacg
    23341 gccgcgatcc ggtccccgcg aattgctacc gcgccgtcct cgatgagacg ccttgccggg
    23401 tccatggtga cgacgtagcg ggcggtccag atccagtccg ccgtttcggc gccggcgcac
    23461 caggccagca gaagaagact acaaagaaga cgcatcaaat ggtctcggga acaggtcttt
    23521 caagcgcaac gactcggtct ttccgcgaag gttcgccagg atcacctcca cgtcgccgca
    23581 gaattcccac agcaactggc ggcaagcccc gcatgggggt gtcagcgtct cggagtcggt
    23641 ggcgatggcg atccgcctga acgtccgcac tccctcggag agcgctttga acagcgcaac
    23701 gcgctcggcg cagacggtaa gcccgagcgt cgcgttctct atgttgcagc ctgtgtggag
    23761 acggccctca gcgtcctcga tcgccgcgcc cacgcgaaat ccggagaacg aggcgcgcgc
    23821 gttctcccgg gcgctcaccg cggcatccac cagtgggtcc atcacacctc cagtcgcggc
    23881 agaagggccg tcaggagctt taccagtgtg cctcgcacca tcacgccggt ctccaggact
    23941 tcctcgtggc tgatcttctg cggcaggatg cccgccgcca tgttggtcac gcaggagatc
    24001 gccagaactt tcattcccat gtggttggcc gcaatgactt ctggaacggt tgacatgccc
    24061 accaagtcgg cgccgatggc ccgaaggtgg cggatctcgg ccggcgtttc ataactcggg
    24121 cccaggagac cggcgtagac gccctcgccc agcgggattc caagctcggc cgccgtttct
    24181 ttcgcgatct cccgataacc ggccgaatac gccaccgaca tgtccgggaa acgcgggccg
    24241 agagattcgt cattcggtcc aaccagcggg ttcgagcctt gcagattgat gtggtccgag
    24301 atcagcacca gcccgccccg gctcagagaa aggtcgatcc cacccgccgc gttcgtcagc
    24361 accagcgccg tgacgccaag tttgcccagc acgcgtacgc cgaaaacgac ttggtccgga
    24421 ctgtacccct cgtacaagtg gacgcgcccc gacatcaccg cgacttcaat tccgcccagc
    24481 cgtccgaaga cgagcattcc ggcgtgaccg atcgccgtcg agtgcggcca gcccggaatt
    24541 ttcccgtacg gtatttcagt gcggccgctg agttcttcgg cgaaagctcc caacccgctg
    24601 ccgagcacga cggcaatccg gggcctgaga ggtgtgcggc ttcgaatttc gtcgatcgcg
    24661 gcttggtaca tgacttacag cagcatgccg gcgatgcagg ccgacataaa gtttgccatc
    24721 gttcccgctg ctacggctcg caggccaagc cgtgcaaggt cggacttccg gcccggagcc
    24781 agagccccga tgccgccgat ctgaatggcg atcgagctga agttggcgaa tccgcacagg
    24841 gcataggtag caatcgtgaa cgatcgcgga tcgagctggt cacggattgg tccgagctgc
    24901 agaaacgcaa cgaactcgtt gagcaccagg cgcgtgccca gcaggttgcc gaccgaggtg
    24961 gcgtctttcc agctcacacc catcagccag gcgatcggag cgaagacaac accgaagatc
    25021 ttctcaaggg actccggaag ccatcccatg ccgggtaatc cgtgggtcca gccgagcatc
    25081 ccgttcacca tcgcgatgag cgccaagaag gcgatcagca tcgcgccgat gttcaacgcc
    25141 aggtgcagcc cgtcgcccgc gccgcgcgcc gcggcgtcga tcacgttcac tcccggcttc
    25201 tcgatctcga cccgcacctt cccggccgtc tccggctgct ccgtttccgg tatcaacatc
    25261 ttcgcgagca gaatggtcgc cggggccgtc atgatcaccg ccgtcagcag gtgcttgatg
    25321 tccacgccgg cgatcttgac gtaggccgcc atcaccgctc cggacacgtg ggccatgccg
    25381 ctggccatca cggtaaatag ttcggactgc gtcagcctcg gaaggaaagg ccggatggtg
    25441 agaggcgcct ccgtctggcc catgaagatg ctggcggcga cgttggtgga ttccgcgccg
    25501 ctcgcgccca tcacgcgctg catcaggacc gccatcccgc gcacgaacac ttgcatcaca
    25561 ccgaagtaat acagcacgga aaacagcgat gcgatgaaga tgacgatcgg cagcacctga
    25621 aatgcgaaga cgacgccgaa aggtccgctc ttcgcaccta atgggccgaa cacgaactgg
    25681 ctgccggctt cggcgtaggc gagcagtgcg ttcacagcca cgctggcggc ctcgaagaca
    25741 cgcccgaagc cggtcttcag aacgaggaac gcaaaggcga actggagccc caggccccac
    25801 agcagcaatt tcggccggat tgcgcggcgc ttcgcggaga acacccatgc ggcgccgaga
    25861 atgacgacga gaccgattag cccggagaac cggcccataa ggatggcgtc agccgactac
    25921 ttcgaggatc aggtcgcgct cttccggcgc ctggttcgag atccggaatg catcctcgat
    25981 gaccgcggcc gcgtcgtcga gccgctcgtc gccggtgtag tagatacggc acaacacggc
    26041 gccggcctcg accttttcac cggccttgac ctcgagaatc acgccgacgg ccgggtcaac
    26101 ctggtcgtcc ttgttgtccc gtcccgcgcc aatcatcgcc gaagccttgc cgacgtcctc
    26161 ggcgtcgatg gcgctgacaa aacccgctcg cggagaggtg atttcccgcg caccgctcgc
    26221 gttgggcagc agctcgaacc tgtcgagcac ctgtggattg ccgccctgcg cttcaatgac
    26281 ttcacgaaac ttgcgatagc ccgaaccgtc gagcagcttt tgctgcgcca gttcgcgtgc
    26341 gtcgtccaga gtttcggtta tcgagccgag atagatcatc cgtgcggcca gttcgagcga
    26401 gatccgcgtc aggtccgtcg gtccgacatt ctgcaaggtc tgggacgctt ccatcacttc
    26461 gagtgcgttg ccgaccgcgt agccgagcgg ctggttcatg tcggtgatga gcgcttggat
    26521 ccgtttgtcc atccggcggc cgatgccgac catcatctgg gcgagccgcc gcgcgtccac
    26581 ttgccgcttc atgaaggcgc cggcgcctac cttcacgtct agaatgatcg cgtccaggcc
    26641 cgctgccagc tttttcgaca tgatggatga agctatgagc gggatcgact cgatggtggc
    26701 ggtcgcatcc cgaagcgaat acaacttgct gtcggccggc gtcacctccg ccgtctgccc
    26761 gatgaaagcg atccccacct gctggagttg cgcacggaac tcgtcgagcg tcaggttggt
    26821 gcgaaaaccg gggattgact cgagtttgtc caaggtgccg ccggtgtgcc cgagcgcgcg
    26881 tccggaaatc atcggaacga tagcgcccgc ggatgccgcc aacggcgccg caatcaggct
    26941 ggttttgtca ccgacaccac cggttgagtg cttgtcgacc ttcactccag gaacggagga
    27001 cagatccaca ctctcaccgg aacgcagcat acagtccgtc agcgcagtaa cctctcggtc
    27061 actcatgccg gaaaagtaca ctgccatcag gaacgccgac atctggtagt cgggaacttc
    27121 accttgcgtg tagccgtcca ccaggaaggt gatttcgttc ggggctaatt cttctccgtc
    27181 ccgcttgcgt tgaatcaagt cgacgattct cattcgtgta agatccttca ggctaccatc
    27241 ttaggagatt gaaagtaaag gaactttttg cgttgaacga cgtcgcctta ctctttcgcc
    27301 ttccggcggc agatgcggcc ggtcagccgg gtttcgaccg ctgccggctc tcagcctatc
    27361 tggggcaacg gcacccggta cttccccgcg aagatctctt cgaacagacc caggatttcg
    27421 gaatggatcg ccgtgttgtc ggccaggagg tgcggactgc tgagattgtg attcgccccg
    27481 tccatgtcgg acaccgttcc cccggcttct gccaccagca gcactccggc agccatgtcc
    27541 cacgggttca gcccgaattc ccaaaacccc tccagccgtc cgcacgctac gtacgccagg
    27601 tcgatggccg ccgacccccc gcgccgtact ccgtgcgtcg ccatcgcgag ttggtggtag
    27661 aagtgcacgt tcacgctcag gtgccttttc cggctcggaa agccggtggc gacaaggcag
    27721 tcctggagtc gggccgcctt cgacacgcga atccggtggt tgttcaaata agcgccgccg
    27781 cccttctcgg cggcgaacat ctcgtcccgc agcggatcgt agatcacccc ggcgatcagt
    27841 tcgctggagc gttccaccgc cagtgtgacg ttgaacatcg gaaacccgtg cgcgaagttt
    27901 gtagtgccgt ccagcgggtc gacgtaccag cggtactccg aagttccgga ctggcccccg
    27961 ccctcttccg cgacaatgcc gtgggtcggg tagcgtgacc gcaaccgctc caccaccagt
    28021 ctctctgacg cgcggtccgc ctccgtcaca aggtcgaact cacccttcag ttcgaacgta
    28081 acgcggcgtt cgtaatagtt atggagcaac gcgcctgctt cgcgcgcaat ctccttggcg
    28141 ctgtcgagta acgatgtcat gactgggatc gggaagctga gcttactcct cgtcctcgta
    28201 cagatacttg atgtcgtgct tgtacaggaa gagattggga ccgtcttgcc gggtcagtcg
    28261 gatgaaattg gcgtcgtaaa actcgatcgt gccggaatac tcctcgccgc tttggaggcg
    28321 aaccgtgatc ggaatcgcgt tctcgatcag acgccgcaga tacttgggct cctcgaaagt
    28381 ctgctcgggc ggttttgcct tcgactttcc attctctttt ttcggcattg ttctttcggg
    28441 ccttacgggg atttctctac cggccgccag ccacgcggcc tgcgcttcga cgagctatcg
    28501 gtctcttcat aatatccgtt gcgcgcgagc acccgcaaat cccggcggga aaccttcacc
    28561 tcgattctgt ggtaacggtc ttgcgaaggc ggcaggtttt tcggatagta ggccaggaag
    28621 tactgcgtcc gaagttcctt gagaatgtcc gagaaggcgc tgtcgagcgc cggcccgacg
    28681 ctcggtgtga agacgcgccc gcccgtaccg gaagatagca gcgtcagggc gttctcgcct
    28741 ccgatgttcc tgcccgcgtc gttggtgatg ggcattacca gaatcgcata cagcacagcg
    28801 tcgctccgct gcgccgcctc gagcgcctgg tgaaagttct tggcgctggt ggtgtctccg
    28861 ccgtccgtga ccaggatgat gacgcgccgc ccttcccgct ggtcgagggc gtccgaagcg
    28921 aggtagatcg catcgtagag cgacgtaccg ctttccggct tcaatgactt catcgcgtct
    28981 tccagacgct cgtggcggtt tgtgaagcgt gcgtgcagcg tgacttcgta actgaagccg
    29041 tacagggcca ccgcatcctc gggatggccc tcggagaaga cggccttcag gaaccggttc
    29101 accgaatcca gttcgtagcg gagctccttc gccgtcgaga ggcttacatc caccagcagc
    29161 gaaatcgaga gcggctgttc ggtgcgccgc tcgaaaaccg agacttcctg cggaactccg
    29221 ctgtcgatga cagtgaagtc cgccttttcg agcgacccca ccaggccgcc cgccgagtcc
    29281 ttgacggtcg ccaggatgcg aacaagactc acgtccaccc gaaagacggt ttcttgggcg
    29341 agcagtagcg ctccggccag aagcagcgcc gctccggcga gcgcgactac cttttgagga
    29401 cggattcgtc ccattcgcct tccacccagg tttcgccgtc ggcgaagtgt tcttttttcc
    29461 agatcgggac ggtacgtttc agacggttga ttccttccag cgccgcctca aaggcgggct
    29521 ttcgatgagg tgcggtcacc acgatgacca cgctcgcctc gccgatctcc atacgtccca
    29581 gcctgtggat cattccgatg tggctgatcg cgtgcgacgc ggcgatctcc cggccaagcc
    29641 gggccatcac cccgatcgcc atcggctcat agcattcgta atcgaggtac agcgtaggac
    29701 ggcccttcgt gttgttgcgg acgacgccct caaacgtaac cacggcgccg tcctcgccgc
    29761 gcaacagtct ggcggccagg gcgcgggcgt cgatcggctc ccgcgttaga gcgaagaaat
    29821 gaccggtttc gctatcctcg acgacccggg caaatccggc gccgccgctg accggaggca
    29881 gaaaagcgat ctcgtctcca tcagcaaccc gctgattcca ttgggagaaa tgctggttca
    29941 ccgccgggac cacactggct ttccactcgg cgagattggg aaagcggcgc gcgaagtgct
    30001 cgaaggccgc accgacttcg gagccatccg aaagctcgat ctgttgctcc gagcagccag
    30061 tcgcctcctt caaaaccccg aaaaacagca ccttgacccg cacactccta gcgtaacaga
    30121 cgggtattct cgaaaacggg ttgcggggcc ggttacattg ataaagtgcc gcccctcacc
    30181 gtccgtgtcc atcccgacga caacgtcgcc atcattgtga accccgaggg cctgccggcg
    30241 gggactgtct tcccggacgg cttgaccctg gccgaggcga taccgcaggc gcacaaagtg
    30301 gcgctccggg atcttgatgc cggtacgccc gtggtccgct acgggcatgt gatcgggtac
    30361 gcaaaccgcc ccgtgcgcgc agggtcctgg gtgcgcgagg acgtcctcga cctgcctgag
    30421 ccgcccgctt tcgccgacct cccgctggcc acgaacgttc cgccatcgct gccgccgctc
    30481 gacgggtgca ccttcgacgg ataccgcaac ccggacggtt ctgcgggcac caagaacatc
    30541 ctcggcatca ccacgacggt ccagtgcgtc gcgccgaccg tcgagtacgc cgtccggcgc
    30601 atcaaatccg aaatcctgcc gcgctatccc ggcgtcgatg acgtcgtggc gatcacgcac
    30661 acgtacggtt gtggcgtcgc catcgacgct ccgggcgcag ccgtgccgat ccggactatc
    30721 cggaatctca gccgcaatcc gaacctcggc ggctcgccgc tcgtcgtcgg tctcggctgc
    30781 gaaaagctcc agcccgagcg gctttttccg gatgcaaagt tgcagccaac cggcgctggc
    30841 ctgcccgtga tcggcgacga cccgtgggtg cttcggctcc aggatccgga tctgcgcggc
    30901 ttcgaagcca tcgtcgccgc gatcctgcgg gctgcggaga aacgtctgga ggaactcagc
    30961 cggcgacgtc gcaagacatg tcccgcctcg gacctggtcg tcggcctcca gtgcggcggc
    31021 agcgatgcct tctccggggt cacctgcaat cccgccgttg gctatgcggc ggacctgctc
    31081 attcgcgccg gctcgaccgt catgttctca gaagttaccg aagtccgcga cgccatccat
    31141 ctcctgactc cgcggtgcgc gacggaagcc atcgcccggg accttatccg tgagatgaag
    31201 tggtacgacg agtacctcca acgcggttcc gccgaccgca gcgccaaccc gacgccggga
    31261 aacaagcgcg gcggactgtc gaacgtggtc gagaaggcgc tcggctccat cgctaaggcc
    31321 ggaagctcgg cgctggtggc agtcgcggct cccggggagc gcgtcaccgc caagggactt
    31381 gtcttcgcgg cgacgccggc tagcgatttc atctgtggcg cccagcaact ggcctcgggt
    31441 atgaacctgc acgtcttcac gactgggcag ggaagtccgt acgggcttgc gctggcgccg
    31501 gtcatcaaag tgtcgtcgcg gaccgccctt gccgaccaat ggcccgacct gattgacgtg
    31561 gacgccgggc gcatcgccac cggaagggct tcgatcgaag acgtgggctg ggaaatcttc
    31621 cggctgattc tcgacgtcgc cagcggccgg aggcagacgt ggaccgaaca ctgggggctt
    31681 cacaacgccc tcgcgatctt caacccaggc ccggtgacct gaaccttact ttacgttctc
    31741 gcgcggattt ggcttctcct gcgccaccgc ctccgccacg gcgaccacgt cgcccaggct
    31801 gtcgtagaca ggcgccagga cggagatcat gtcgggccgg tcggtaacgg tcaccctctt
    31861 accggtctcg agaacggcct tcagccgcgg cggggtgtcc tgatgaagga ccccccagga
    31921 ccagaattgg ctcatctcgt ggacgttccg gatgacgatc tggatcctgc ctggcattcc
    31981 ttccaccggc gaccagagat tcacgtcgca cggaaacgag gcgagcttcg acagccggat
    32041 cagcgtcttc ttcacttgaa tatatggctc gtggttgacg tcgaagttgt cgctggcaac
    32101 ccatgggtct ttcggatcga ctttacgaaa cgattcgagc gcgcgtttcg tcacgatgcg
    32161 cttggcggcg tctccgtcca ccataaccgt ggctactcgg ccgatctcgt ccaactgcct
    32221 cgcgatctcg gagggagtct cggccggcag gcacgcggca aacagcacga atgttacgaa
    32281 ggctctcatc atcactttcc ccccacggcg agttgcagaa tccacacccg tactttctcc
    32341 caggactgtg catcgacgcg tgcggccagg tccttcaccg gcttggagtt ctcggcgatc
    32401 gtggcatttg tcatctcgta gggcggcaga tcggcggcag cgggccgcgg cggccaccag
    32461 tcctcgtagc gcgaactgtt gtaacggctc gcggctcctg cgcgcagttc ttcgaagggt
    32521 ttcctggtct taaggctgtc aagcagcgtc aggtcctgca cgcaatttcg ttgcagcttc
    32581 agccgaacgc tcggaatcgg cccgtcgata gcgaagcggt cgcccgaata cgccagcaca
    32641 gtgtctccgc ctccgaagcg gaaccacggg tccgcaccgg gagaaaccgt cagccaccgg
    32701 acgaatccct ccgcgcccca gatccacgtc tggaatggtg cgaaggtgat agccccggaa
    32761 ctttccgtta cctccggtgt cccgccgtac atccagacga tgtcgccgcg cgccctcagc
    32821 attgcaggga cctctttgta ccagctcagc tccgatcctc caaggaccca gaagtcgatc
    32881 acgcccgcca gctctttgaa ctgctgtccc atcatccagc tcgaatcgga gcggaagcgg
    32941 aagtgaacct gcgagccggg cggtacagct ttcttcagca gccgggcata ttcggcaaag
    33001 tagcggttgt cgttggggaa acgtgtctcg tcgccgtccc acggaaacgc tttgtagcgc
    33061 ttcttgtgat tgaagaacat ctcgaaagcg gtcttcgtcc agcccttgtc ccgaaaatgc
    33121 tgctccatct cgcggacgac gttcacgaat tccgtctcgt agccgggctc gccccaccac
    33181 agaaacgacg ccggccactc gggattgatc ggaaggtaga cgaacgggat cggttgagcg
    33241 cctcgcctcg tgccggcaaa cgcggagccg tcgagcagcg gaccgtagtg ccggtcgtac
    33301 aggctccagc tcttgattcg ctttgttcgc cccgagccgt cgagttccgg cgcgaactcc
    33361 ggaccgacct tcccgccgtg cccatatccc aactggtgga agacgccgag gtgctcatag
    33421 aaaatccgat ggtaggagtg gatcagtccg aagaactcgt cgcttgcgaa gaacttctcg
    33481 cccagccgct gccgcagctt cggatactgg ctggagagcc actcgctgcc gtacgagtta
    33541 tggtcgatcg ccacgatttc cttctcggga atcgaggcag gcagcacctg aagctcgatg
    33601 cggcgcgaca ccttctttcc gtcgccctcg attctcgctt caccccgata gacgccggga
    33661 gcggtgtccc gcggaatcca gatatccacc cagaacgtct ggacggtctg tttctcgatc
    33721 ctgttgtccg gctctggtag ctgcgaccgg tacggcagtt tcaccggggc gagcgcgtcg
    33781 ggataatacg aattcttcgg ttccgtgtag tgaaaccact cacggaaggc ttcggccgtc
    33841 aggttggtcg ccggcgtgtt ccacgcaaat gtcaccgaat acggccccgg cttcgaaagc
    33901 cttgcggcaa gacggaacga cacgtaaccg ccgcgggcgc cccgcagcgc gaccactccg
    33961 ttcggacccg agagcggctg gccggcgggc cggtccgctt caatgaccac gccaaaagga
    34021 tcgagcctcg cgaactcgga atacgcctcc agatccgctg cccggaggcc gaaggcaaat
    34081 atgcaggaga gaaagatcag tgcacgcaca ggattatcgt agcagggcta taattcgaca
    34141 cgatgcgaac gcacgaaacg agcctccgtg tccgctacgc ggaaacggac cagatgggag
    34201 tggcctacta ctccaactac ttcgtttgga tggaggtcgg gcgggttgaa tactgcaagg
    34261 ctaccggctt ccgctaccgc gacatggagg aacaggacgg cgttctactg gcggttgtcg
    34321 gcgccgaatg ccgctacctc tcaccggcgc gctatgacga cgaagtaatc gtcaaaacct
    34381 gggtcgagca agccaatccc cggatgatcc gcttcggcta cgagatgcgc gaggcatcgt
    34441 cgggccgcac gcttgccacc ggcgagacaa agcacctctt ctgcggccgc gacatgaagc
    34501 cagtcaaatt gcctgagaaa tactgggccc tcataggaat ccgctcctag agatgtccca
    34561 tcctgagtca ctcgtctcca tagacgttgc ccccgcgata gacggcgcag gcatccgcat
    34621 tgcgcggaga agggcgacgc aattctgact ctgtcgacgg ctgcctacga ctggctttgg
    34681 tccgagtgcg gctgaagtag acgttctccg gactgcgaat caccggctga cgcggcatcg
    34741 tgtgggtatg aattggagcc ggatttccac atctcgatgc ccaggccagc gaacgcaacg
    34801 ccgaagatca cgccagacca gacgaagaga gcattcgcgc cccgaacgta actcccgatg
    34861 aggccgatga gcgtcgagag caggaatgcc acgggaaccc agccacgaat ccagcgtgcg
    34921 atccacgccc tggcgctgag aaggatcacg ccgcacgcag tcgccaacgc tcccgctggg
    34981 tatagatcga cggtcgaaat cgcccgattc gctctcacgg tcgcgaggcc aacaagcgcg
    35041 agagcgaggc cagccgcgcc tggccatccg atgctcttag agtgccgcgc actaaaaccc
    35101 acgagcccta tagtcagaca cacgtcaacc acgatgtaga gcgactcacg ctcgatatca
    35161 gaaccgatga ccaccggtgc aaacgaggca gccgctcgta gcgctccgcc cgcgatcgct
    35221 gccagccctg ctgcacgaac gagagtcgac cttgtcatac accagacctc ctgaccaaat
    35281 gtatcgtagt ggccgcgtct gcctcggtgg acgggcgaaa agcccggcgc tttggctgtt
    35341 cccatggcac caaactccga tgcggaccct taagctcctg acagccaata cgcccgagac
    35401 acatttgcgg agactgatgg aaacccaaca caaatgagtg ccgtaccgat acgaacgagc
    35461 ccccccggcg gcagcattga tagcgccagc ggtcatcccg gagaaagaca ccgaacgact
    35521 cgatcatcag gaatctcccc ttgtagcttt cgtggagtta ccggctgcgc tgcttctgga
    35581 tttcaggcat caagcttcac ggcgccgtcc gcgtgcccca cgcacgtcag cacaaagcct
    35641 tgcgctctcg aatcgggatc tagtccctct tgcgtgtcca ttcgcacatt tcctgccagc
    35701 agcttcgttt tacaggtccc acactgtccc tgacggcagg atgaagaaat gccaacgcca
    35761 tgctcctcgg ccgcctccaa taaggtctgg tcgctgcgga tggcgtacgc tttgcctgac
    35821 cgagcgaact cgaccactac gtccgtctcg gccgccgccg aaaccggttg agcactcttt
    35881 ggagcggagc tgccgaaact ctcctgtctg atacgttccg gcttcactcc caggccaatc
    35941 aggatcgcgc ggctggcttc catgaacgga ggtgggccac aaagaaagaa gtcctgtaat
    36001 gcaatttctt tgagggtatt ttccatgaat tcacggctga catgcccgcg tggaccaggc
    36061 cactccgtgt gaggttgcga aagcagaagg tgatactgaa agttcttcag gcgtgcccgt
    36121 aactcttcca actcaccgtg gaagatgatg tcgttactag tgcggacgca atacagcaaa
    36181 gtcacagttg tttcgagaca gaggtcatcc atatagcgga gcatcgccat catgggtgta
    36241 atcccgcttc cagccgcaag cagaacgacg ttctggtgtt tgctctcgtc aaaacagaag
    36301 tgcccgaagg ggccattcgc ttcgaccgtc attccaacgg tagcccgatc gttcaagaac
    36361 accgacacgc agccctgact tactcgcttt g
//
LOCUS       DATIFQ010000127         5851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1765659, whole genome shotgun sequence.
ACCESSION   DATIFQ010000127 DATIFQ010000000
VERSION     DATIFQ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765659"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VLE22_05755"
     CDS             <1..941
                     /locus_tag="VLE22_05755"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13943.1"
                     /translation="GKYAATEVPAALTIEECWELVNTSEQTGMPCMMLENDCFGRGAL
                     MALNLVQQGVLGELIHCEGGYDHDIRAGLLRNGELSWRGMHALRRNANLYPTHPIGPI
                     AWWTGINRGDRFTYLTSMSSKSRGLNRYAEKHFGPDNVNAKRKFVNGDVNTSVIMTEK
                     GVTVTLNHDTQLPRPYSDSGDTSIPLMVQRLQGTEGIFFGSMEKIYIDGRSPLHKWED
                     TGPYYEQYEHLLWKELGEMAKSSSHGGEDYVELYSFVRAVRNKIQTPVDVYDAATWSV
                     ITPLSEQSVASRSAPVDFPDFTRGKWKTVRPIDLGL"
     gene            1194..4406
                     /locus_tag="VLE22_05760"
     CDS             1194..4406
                     /locus_tag="VLE22_05760"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB13944.1"
                     /translation="MTNSKFAVYLIAAFLLLQCNLSAQFTTASLNGTVVDPSGAAVPE
                     AKVAIHNTETGFQTVTTTGDTGLYVFPRLPVGNYTLRVEKGGFATYVQEGINLTVNQS
                     ATQNVSLKVGQIAESVVVTSNVELVTTGTATVGQLVDEKRVVELPLNGRRAQTLLYLA
                     AGTVDEDQIRSLGYGGVYPGEQLANINGTGLGQVNYQLDGAGHNDTYLNLNLPFPNPD
                     AIQEFNLQSDNLSAQFGNAAGGVVNIATKSGTNEIHGGVFEFLRNGSLNARNYFAPVH
                     DSLKRNQFGGNVGGPIKKDKLFYFGTFQGTRIRTAPEGQIAYVPTQAQRDGDFSEIST
                     QLVDPLNGQPFSGNQIPGTRLSPVAKNLLKGIPLPTGPGGEVNYTGRSGDLNENQFMI
                     KGDYNAGKHQFNARYFFSDYNDLGSFSAENLLAASSNINGVRVQNVAANYTYVARPNL
                     MINSWFGWNSQRGGSIPNATFGFPDAGMVIATPDFPELDFTVSDYFTVGTSGAGDFDR
                     GDWTLREDVSWIKGGHEMHYGAEVVRITNDLANPWLQNGNFTFGNQLSGDNLADYMLG
                     AVSEFIQGAGEYKNMVGTRLSFYVQDHWRVNPRLTLNLGFRWDPSTPYRETKDRILCF
                     VPGAPRSQRYPNAPLGLAYGGENHDPGCPEAGYDSNLMNLAPRVGFAYRLTSDGRTSL
                     RGGAGFYYTPVMSALVYYVGDQPFGPIYDFTGVVSFDDPWKSLGIQSPFPRLYGAFVP
                     GADAEFVTPAWLYYMQKDFRLPQLFTWNLTLERQIGQSWVVRAAYVGNKGTHISGSDD
                     YNPAQEKNPAVYIPGASTADNTQERRPYQDFSNINEIGSNNNTRYQSAQLTAERRFSR
                     GLSVLANYTWSKLLDDIGWTNPFNRAFDFGRSRDDLGQNFKLSSIWEIPNTRLSGAAG
                     GLLNGWGLTTNLTWHGGFPFNLRSGRDNSRSGVGRDRPDFVGTDIHDAQLDSGRSHAE
                     MIAEFFETSLFEQNAEGTYGNAGKNVLLGPRFFNADWGVLKNTRITERTNLQFRAEFF
                     NLFNNVNFKLPNSRKTSSSFGRITSAFDPRIIQFGLKFSF"
     gene            4711..5373
                     /locus_tag="VLE22_05765"
     CDS             4711..5373
                     /locus_tag="VLE22_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13945.1"
                     /translation="MQLVPTQGDVIDLLSKTGALRDGHFACPSGFHTNRYLETALAMR
                     YYHHAKTLSVGLSRLLRADSELRAILPELSIVAATPAGLPVAYGLCESLRARQVYWTE
                     KPSPAEPMRFRQYLEPSPGEKVVLANDILRSGVLVGEARALLESRGARVLALAALVYQ
                     PTPRTPDLGPLPLYYLAKLEVSYYADAASCELCKRGAPIERIGRESVEQVLEAHAAVG
                     AF"
     gene            5397..>5851
                     /locus_tag="VLE22_05770"
     CDS             5397..>5851
                     /locus_tag="VLE22_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HSB13946.1"
                     /translation="MSSKHVSGIVDRHRGNRGALIAILQDIQARFGYLPAEALKTVAE
                     ETGRPLTDVYGAATFYRAFSLKQRGKHVCSVCLGTACHVRGGGVIADEFTRQLEVKPG
                     GTTQDREFTLETVNCLGACALGPIVVVDGHYFSNVSPVRVKSILEQARDG"
BASE COUNT         1253 a         1676 c         1806 g         1116 t
ORIGIN      
        1 cgggcaagta cgcagccacc gaggttccgg cggccctcac gatcgaggaa tgttgggaat
       61 tggtgaatac ctccgagcaa acgggcatgc cctgcatgat gctggagaac gactgtttcg
      121 gccgaggcgc gctgatggcg ctcaacctcg tgcagcaggg cgtgctgggt gaattgattc
      181 actgcgaagg agggtacgac cacgacatcc gcgcagggct tttgcgaaac ggcgagctgt
      241 cctggcgcgg gatgcatgcc ctgaggcgca acgcaaatct ctatccgacg catccgatcg
      301 gcccgattgc gtggtggacc ggcatcaacc gcggcgaccg cttcacgtat ctgacctcga
      361 tgagcagcaa gtcgcgcggg ctgaatcgtt acgcggagaa gcacttcggt cctgacaatg
      421 tgaatgcaaa gcgcaaattc gtcaacggcg acgtcaacac cagcgtcatc atgaccgaaa
      481 agggtgtcac cgttaccctc aaccacgaca cgcaattgcc gcgtccgtat tccgattccg
      541 gcgatacaag catcccactg atggtgcagc gccttcaggg cacggagggg attttcttcg
      601 gaagcatgga aaagatctac atcgacggcc gcagtccgct tcacaagtgg gaggataccg
      661 gcccgtacta tgaacaatac gaacaccttc tctggaaaga actgggcgag atggcgaaaa
      721 gctcgagcca cggtggcgag gattacgtgg aactgtactc gttcgtcagg gcggtccgta
      781 acaagattca gacaccggtc gacgtgtacg atgcggccac ctggagtgtg atcactcctc
      841 tgagcgagca gtcggtcgct tccagaagcg ctccggtgga ctttccggac ttcaccaggg
      901 ggaagtggaa gaccgtgcgg ccgatcgatc tcggtttgta gccgggttcg gcgttgcgga
      961 gaatcatcgg taaagccttg tttatcaatt ggttagcacg ggattccatc cctaccgtca
     1021 caccgtcaaa ccattgacaa ggctccggga ttgccctaga ctatacgtgt ttatccgggg
     1081 atcgggggcc gcacattcag tggttgttgg gtagatcgtg taaaggctga gaaccgcgga
     1141 agcggttcgg cgttgccccc gatatgtgct gaggccatca tagggggcga ccaatgacca
     1201 acagcaagtt tgcagtctat ctgattgcgg cattcctgct gctgcaatgc aatctatcgg
     1261 cgcaattcac cacagccagc ctgaatggca ctgtcgttga cccttcaggc gcggccgtgc
     1321 ccgaagcgaa ggtagccatt cacaacaccg agacaggctt tcagacggtg accaccaccg
     1381 gcgacaccgg cttgtacgtt tttccccgtc tgcccgtggg gaactacacg ctgcgggtcg
     1441 aaaaaggcgg ctttgcgacc tacgtccagg aggggatcaa cctgacggtg aaccagtcgg
     1501 ccacccagaa tgtttccctc aaggtggggc agatcgccga gagcgtggtc gttacgtcga
     1561 acgtcgagct cgtcaccacc gggacggcga ccgtcgggca gttggtcgat gagaaacggg
     1621 tggtggagtt gccgctgaac ggccggcgcg cccagacgct gctctatctt gcggcgggaa
     1681 ctgtggacga agaccaaatc cggagcctcg gctacggcgg ggtgtatccg ggcgaacaac
     1741 tcgccaacat taatggaaca gggctcggtc aggtgaatta ccagctcgat ggcgcagggc
     1801 acaacgacac gtacctgaat ctgaacctgc cgtttccgaa ccccgacgcg atccaggagt
     1861 tcaatctgca atcggacaat ctgagcgcgc aattcggcaa cgccgccggc ggcgtggtga
     1921 acatcgcgac caagtcgggg accaacgaga ttcacggcgg cgtcttcgaa ttcctccgga
     1981 acggctcgtt aaatgcccgc aactactttg cgcccgtgca tgattcgctg aagcgaaacc
     2041 agttcggcgg caacgtgggc gggccgatca agaaagacaa gctgttctac ttcggcacgt
     2101 tccagggaac acggattcgt accgcacctg aaggtcagat cgcctacgtc ccgactcagg
     2161 cccagcgcga cggcgatttt tccgagattt cgacccaact ggtggatccg ctgaacgggc
     2221 agccgttctc aggcaaccag atcccgggca ccaggctgag tccggtcgcc aagaacctgc
     2281 tgaaggggat tccacttccg acgggtccag gtggcgaagt gaactacacg gggcgcagcg
     2341 gtgacttgaa cgagaaccag ttcatgatca aaggggatta taacgccgga aagcatcagt
     2401 tcaacgcgcg gtattttttc agcgactata atgacctggg ctcgttttcg gcggagaatc
     2461 ttctggccgc cagcagcaac atcaatggcg tccgggtgca gaacgtggcg gcgaactaca
     2521 cctacgtcgc gcggccgaac ctgatgatca actcctggtt tggctggaac tcgcagcgcg
     2581 gcggttccat cccgaatgca acgtttgggt ttccggatgc cggcatggtc attgcgactc
     2641 ccgactttcc agaattggac ttcacagtaa gcgattactt caccgtgggt accagcggcg
     2701 ccggggactt tgaccgcggc gactggacac tgcgcgagga cgtgagctgg atcaagggcg
     2761 gccacgaaat gcactacgga gccgaagtgg tccggatcac gaatgatctg gccaatccgt
     2821 ggctgcaaaa tgggaatttc acgttcggaa accagttgtc cggcgacaat ctggcggatt
     2881 acatgctcgg agcagtctcg gagttcatcc agggcgccgg tgagtacaag aacatggtcg
     2941 gcacgcggtt gtctttctac gtgcaggatc actggcgggt aaacccgcgg ctgacgttga
     3001 acctcggctt ccggtgggac ccgtctacgc cttacaggga aaccaaagac cggatcctgt
     3061 gcttcgtgcc gggggcgccg cggtcacagc gatatccgaa cgcaccgttg ggactggctt
     3121 acgggggcga gaaccacgat ccgggctgcc cggaggctgg ctatgacagc aacctgatga
     3181 acctggcgcc ccgtgtcggc tttgcttacc ggctgaccag cgatggaagg acgagcctcc
     3241 gtggcggagc cggcttctac tacacgccgg tcatgagtgc actggtttac tatgtcggag
     3301 accagccgtt tggaccgatc tacgatttca ctggcgtagt ctcgttcgac gacccgtgga
     3361 agagccttgg aattcaaagc ccgttcccga ggctgtacgg ggcgttcgtc cctggcgcgg
     3421 acgcggagtt tgtgacgccg gcgtggctgt actacatgca gaaggacttc cgcctcccac
     3481 aacttttcac ctggaacctg acgctcgaac gacagatcgg gcagagttgg gtggtccggg
     3541 cggcgtacgt ggggaacaag gggacgcaca tctccggctc ggacgactac aatccggccc
     3601 aggaaaagaa ccccgcggtc tacatcccag gggcctcaac ggccgacaat acgcaggaac
     3661 ggcggccgta tcaggacttc agcaacatta acgagattgg ctcgaacaac aatacgaggt
     3721 atcagtcggc acagttgacg gcagaaagac gtttcagccg cggcctctca gtgctggcga
     3781 attacacctg gtccaagctg ctggacgata tcgggtggac caacccgttc aaccgggcat
     3841 tcgactttgg gaggtcccgg gacgaccttg ggcaaaactt caagctttcg agcatttggg
     3901 aaatcccgaa cacacgctta tcgggtgcag cgggcggttt gctgaacggg tgggggctta
     3961 cgacgaacct gacctggcac ggtggttttc cgttcaatct tcgcagcggg agggacaact
     4021 ctcggtcggg ggtcggacgc gaccgtccag acttcgtcgg caccgatatt cacgacgcgc
     4081 aattggattc cggccgctcg cacgcagaga tgatcgccga gttcttcgaa acctcgctat
     4141 tcgagcagaa tgcggagggt acgtacggga acgcgggcaa gaatgtgctg ctcgggccga
     4201 gattcttcaa tgccgattgg ggagtgttga aaaatactcg gattacagag cggaccaacc
     4261 tccagtttcg ggcagagttt ttcaatcttt tcaacaacgt gaatttcaag ctgcctaact
     4321 cgcggaagac ttcgagcagc tttggccgca tcacatcggc gttcgaccca cggatcatcc
     4381 agtttggttt gaagttctct ttctgagcgg acgcaaccgg ggcagggcag caggccgtcg
     4441 ctctcaacga acgccggcct gctcatttcc ttagcactaa taatcccgga ggattcatgc
     4501 cgtgatggcg ctgggtgatg ccgccagcgc tactcgcatt tgtgtgtggc gtcgctcaaa
     4561 atagcgacag gatgcgtaac ccccgcttca tcagccagat cctggtctgt gagtaccaca
     4621 gacccattga cgcaccacgg cggacggcag tataaacaga tagccgagtc tgatttcgcc
     4681 tgccttaccg ggcagggcgg gaggtgagtc atgcaactcg tcccgacaca aggggacgtg
     4741 atcgacctgc tgagcaagac cggcgccctt cgagacgggc actttgcctg cccgagcgga
     4801 ttccacacga accgatacct ggaaacagcc ctcgccatgc ggtattacca tcacgcgaaa
     4861 acacttagcg tgggtctgag ccgcctgttg cgcgcggact cagaactgag ggcaattctg
     4921 ccggagcttt cgatcgttgc ggccacaccg gccggactgc cggtggccta cgggttgtgc
     4981 gaatccctga gggcgcgaca ggtttactgg accgagaagc ccagtccagc ggagcccatg
     5041 cgcttccggc agtacctgga accgtcaccc ggagagaaag tcgttctggc aaacgacata
     5101 ctccggtccg gcgtcctcgt gggcgaagcc agggcactgc tggaaagccg aggcgcaagg
     5161 gttctggcgc tggcggccct ggtctatcaa cccaccccaa ggactcccga cctgggaccg
     5221 ctgccgctct attacctggc caagctggaa gtgagctact acgccgatgc tgcctcctgt
     5281 gagctgtgca aacggggcgc accgatagag aggatcggga gggaatctgt ggaacaggtt
     5341 ctggaagcgc atgccgccgt gggcgccttt tgaaagggaa acaacgatcc gctgatatga
     5401 gttcgaaaca tgtctcaggc atagtagata gacacagggg caaccggggt gcactgatag
     5461 ccattcttca ggacatccaa gcccgcttcg ggtacctccc agcggaggcg ctgaagactg
     5521 tcgcggagga gacgggccgc cccctgaccg acgtttacgg cgctgcaacc ttctatcgcg
     5581 cgttcagcct gaaacagcgg ggcaagcacg tttgctcggt ctgcctgggc acggcttgcc
     5641 acgtgcgcgg gggaggggtc atcgccgatg agtttacgcg gcagcttgaa gtcaagccgg
     5701 gagggaccac gcaggatcgc gagttcactc tcgagacggt caactgtctg ggggcgtgcg
     5761 ccctgggccc catcgtggta gtggatggcc actacttttc caatgtgagc ccggtgcgag
     5821 taaagtcgat cctggaacag gccagggacg g
//
LOCUS       DATIFQ010000128         5319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1382777, whole genome shotgun sequence.
ACCESSION   DATIFQ010000128 DATIFQ010000000
VERSION     DATIFQ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382777"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2126
                     /locus_tag="VLE22_05775"
     CDS             <1..2126
                     /locus_tag="VLE22_05775"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSB13947.1"
                     /translation="DRHLQSRIEKAVEADKKEWERVKTLADWERFRDPRLQALRASLG
                     EFPARTPLQTRVIKDVPEQGYRREDLVFQSRPGLWVTANLYLPSKPPNRMPGIVIIHS
                     HHRPRTQAELQDMGILWARAGCAVLVMDQVGHGERIQAYPWNRDAYHARGLTGMQLYL
                     VGESLVKWIVWDVMRGIDFLLERKDIDPERIILLGAVAGGGDPAAVAAALDSRIAAVA
                     PFNFGRRGRDWGAGWGEWESTRCLRRSIVDQFLPWLICASIAPRRLVYSNEMGWDGYN
                     TDRAWLDYQQIFSFYNARDSLEEAHGFGTFPGPGECTNIGPAQRGTLYPELKRWFGIP
                     IPTEEPDDRRPEVELAALNPSVASQLGMRKIHELAQEIAAAKLNAARAELRKLKPEAR
                     REWLQARWAARLGDIEPNRLPEATLHWKKHWADAEVEGITLEVESGILVPLLLLGPAT
                     TVNTRLPVVVAVSQGGKERILAERSREIEALLKGGTAVCLPDVRGTGETSPDQRRGLM
                     GEERSAAATELMLGNTLLGARLKDLRTVLAYVRGRRELDPQRVCLWGESLVPVNPPRL
                     LLEETLWWQVGPEIQHQAEPLGGLLALLGALYEDGVRTVTVRQGLSAYLSILESPFAY
                     VPYDIVVPGIFDAGDVEDLAASLAPRPLLLEGLVDGRNRLVPEGTLRTRLAMIYESYR
                     ASDAPLLIRSREGTPSLTQWILARL"
     gene            2409..4655
                     /locus_tag="VLE22_05780"
     CDS             2409..4655
                     /locus_tag="VLE22_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB13948.1"
                     /translation="MAAVRSTAKGVPGGLSWGDADDLRERAPSVEKLAIYSPRTWGVQ
                     TERQGHVEVLLSAMTTGEFFEVLGVSPALGDPMTRAHDAIGAQSEAWLANAAAARLFG
                     HPEASLHRTLWINGAPYSVAGVLPAGFRFPMAQGTPDMIIPLSRAEYCCARGAGAQQA
                     IALLRDSGRFDAELRVASNTLARDFPATNAGLRFVPVALREYLFGPRILALRWILAGA
                     LCLTIIAAANGSGIWMARWLRVRRDMAIRLSLGASAPRLAAVRATEGAIAGFAAGAVG
                     LAIASAILRVAQAVPFLRDRLEAFAVWRPVSLESAAIPGALMVGVGVGLIAALLPHIA
                     VMGRLAREGTCEGMSTHPAARRVRLVLTAVQLTATAILGWTAITIGENVHTLLTARRG
                     FETEETLIAGIGLPEARYDTDEKMIGFHAAAIAQLQAVPGVTAAAGGVNVPQGNMRTR
                     FLRDGQTLERDRQPMARIGVVSPELLPLLRISLRRGRGFTSDDRWSAPRAALVNEAFV
                     RGYLADSEDPLREGLRLSFYNGFAMKPYTRFQIVGIIADTRNDGLLVDPQPQILIPAA
                     QIAMEGFFYYVKTARPAASVQTELREAIWRVDPAIERVTFSPLNDYVEQGLAERRALS
                     AFGLLVLLVAAVIVAAGLFASLSASLLESTRELAIRAALGATPARLAVESLRWALVAV
                     GLAGVVTAVAVPFVASSVPLEKAILRPTPASVTLCLLTMGVVTGAAALRPAWRAASVS
                     PADALRAQ"
     gene            4764..5183
                     /locus_tag="VLE22_05785"
     CDS             4764..5183
                     /locus_tag="VLE22_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13949.1"
                     /translation="MRFGLIFPVLLAAALNADPPMKPRVFLAGAEPERISGEALLGES
                     RGALELTRSTPSYDIESMRTFTRQCPGVVVTTRRDKADIMIRVERDDPNPTTPFVKAN
                     KIAVFNLNDELVYATRARLLSNACKDACRAILNYVKQ"
BASE COUNT          903 a         1631 c         1760 g         1025 t
ORIGIN      
        1 ttgaccgcca cctgcagagc cgtatcgaga aggctgtgga ggcggacaag aaagagtggg
       61 agcgcgtgaa aaccctggcc gactgggaac gattccgcga tccgcgcttg caggcgcttc
      121 gagcgtccct gggcgagttt ccggcccgca ccccgctcca gactcgcgtg atcaaggacg
      181 ttcccgagca ggggtatcgc cgggaggatt tggtctttca gagccgtccc ggcctttggg
      241 tgaccgccaa tctctacctg ccctccaaac ccccgaaccg catgccgggg atcgtcatca
      301 tccatagcca tcaccggccc agaacgcaag cggagttgca ggacatggga atcctttggg
      361 ctcgggcggg ctgcgctgtc ctggttatgg accaggtcgg ccacggtgag agaatccaag
      421 cctatccctg gaaccgcgat gcctatcacg cgcgcggttt gacgggcatg caactctacc
      481 tggtgggcga aagcctggtc aagtggattg tgtgggacgt catgcgcggt attgacttcc
      541 tcctggagcg caaggacatc gatccggagc ggatcatttt gcttggagcg gtggccggcg
      601 gcggggatcc tgcggcggtt gccgccgcac tcgactcgcg gatcgcggca gtcgccccat
      661 tcaatttcgg caggcggggc agagactggg gcgccggctg gggcgagtgg gaatccacgc
      721 gctgcctgcg acgcagcatc gtcgatcagt ttctgccgtg gctgatctgc gcttccatcg
      781 cgccccggcg cctggtgtat tccaacgaaa tgggttggga cggatacaac accgaccgag
      841 cctggctcga ttaccaacaa atcttcagct tctacaatgc ccgcgacagc ctggaggagg
      901 cccacggctt cggcactttc ccgggaccgg gggagtgtac caatatcggg ccggcccaac
      961 ggggaaccct ctacccggaa ctcaagcgtt ggttcggtat tcccattccc accgaggagc
     1021 ctgacgaccg ccggcccgag gtggaactgg ccgccttgaa tccgtccgtc gcttcgcagc
     1081 tcgggatgcg aaaaattcat gagctcgctc aggaaatcgc cgccgcgaag ctgaacgcgg
     1141 ctcgcgccga actccggaag ttgaaacccg aggcgcgccg tgaatggctc caggcccggt
     1201 gggcagccag gctcggcgac atcgaaccga atcgccttcc cgaagcgaca ctgcactgga
     1261 aaaagcactg ggcggatgcc gaagtcgagg ggatcaccct ggaggtcgaa tccgggatcc
     1321 tggttccact gctcctgctt ggccctgcga cgacggtgaa cactcgcttg ccggtcgtcg
     1381 tggccgtttc ccagggcggc aaggagcgaa tcctcgcgga acgcagtcgt gagatcgaag
     1441 cgcttctgaa aggcggcacc gcagtttgcc tgcccgacgt gcgcggcacc ggtgaaacct
     1501 cgcccgatca gcggcggggt ctcatgggcg aggagcggtc ggctgccgct accgagctga
     1561 tgctcggcaa cactctgctc ggcgctcgtc tgaaagacct gcgtaccgta cttgcgtatg
     1621 tgaggggccg gcgggagctt gacccacagc gggtctgcct ctggggagaa tccttggtcc
     1681 cggtgaatcc gccccgcttg ctgctggagg aaaccctctg gtggcaagtc gggcccgaga
     1741 tccaacacca ggcggagcca ctggggggcc tactggccct cctgggcgct ctctacgagg
     1801 acggagtgcg cactgtcacc gtgcgccaag gcctgtcggc ctacctgtcg attctcgaaa
     1861 gcccgttcgc ctatgtgcca tacgacatcg ttgttcccgg cattttcgac gcgggcgatg
     1921 tcgaggacct ggcggcgagc ctggcgccgc ggccgttgct gttggagggc ctggtggatg
     1981 gacgaaaccg cctggtacca gaaggaactc tccgcactcg actggcaatg atttacgagt
     2041 cttaccgagc ttccgatgcc cctctgctga ttcgaagccg agaagggacg ccgagcctca
     2101 cccagtggat cctcgcacgt ttgtaaggcg gatcacatca accggagccc gatccggcgg
     2161 agggttttgg cgcggttctt tctgagtaca ccataatttg tggcactggg cttgacggat
     2221 cgacaatact taaacgaatg ccgatactcc tcttactggg tgaggcggta cgcgaacgcc
     2281 gccgcgattg gcgagtaact gctggcttct gcggcgtgct gggaagctct ttggccgttg
     2341 cggtcgcatg cttttcgtta gcttggcctc ttttgtggga accgctgccg ttcccgaacg
     2401 caagacgggt ggcggcggtc cgctcaacgg cgaaaggcgt cccgggagga ctgtcctggg
     2461 gtgacgcaga cgatctgcgg gaacgggcgc cgtcggtcga aaaacttgcc atctattcac
     2521 cccgaacgtg gggcgtgcaa acagagcggc agggccatgt ggaggtcctc ctgtcggcca
     2581 tgacgacggg cgaattcttc gaagtgctgg gcgtcagtcc cgctctcggc gacccgatga
     2641 cgcgcgccca tgatgcgatc ggggcgcaga gcgaggcttg gctggcgaac gctgcggctg
     2701 cgcggctgtt cggccaccct gaggcatcac tgcatcgcac gctttggatc aatggcgcac
     2761 cttacagcgt ggcgggtgtg ctgcccgctg gctttcgttt tccgatggcc caaggcacac
     2821 cagatatgat cattccgctc tcgcgcgcag aatactgctg cgcacgaggc gccggcgccc
     2881 agcaggccat agcactgctg cgagactcag gacggtttga cgccgagttg cgggtcgcct
     2941 cgaatacgct ggcgcgcgac tttccagcta caaatgcggg gctgcgattc gtgccggtcg
     3001 cattgcgcga atatttgttc ggcccgcgta ttctggcatt gcgctggata ctcgccggtg
     3061 cgctctgcct cacgattatt gctgccgcca acggttctgg aatctggatg gcaagatggc
     3121 tgagggtccg gcgcgacatg gcgattcgct tgtcacttgg cgcatccgcc ccgcggctgg
     3181 ccgctgtcag agcgaccgaa ggcgcgatag cgggcttcgc tgccggcgct gtcgggttgg
     3241 ctatagcgtc ggcgatactg cgcgtggcgc aagcggtgcc atttctgcgt gaccgcctcg
     3301 aagcgttcgc cgtttggcgc ccggtttcgt tggagtccgc tgcaattccg ggcgccctca
     3361 tggtgggagt tggcgttggc ttgatcgccg ccctgctacc acatattgcc gtaatgggcc
     3421 ggctggcgcg cgagggcaca tgcgagggga tgtcgacaca cccggcggcg cggcgcgtgc
     3481 ggctggtgtt gacggcagtc caacttacgg ccacggcgat tctcggctgg accgccatta
     3541 caatcggcga gaatgtgcac acgctgctga ctgctcggcg cggatttgag acggaggaga
     3601 cgctgatcgc aggcatcggg cttccggaag cgcgttacga cacggatgag aaaatgatcg
     3661 ggtttcacgc ggcagcgatc gcgcaattgc aggcggtgcc gggcgtaacc gcggccgcgg
     3721 gtggtgttaa cgtaccgcag ggcaatatgc gcacgcgatt cttgcgcgac ggccagacgc
     3781 tggagcgtga ccggcagccg atggcgcgga tcggagttgt cagcccggag ctgctgccgc
     3841 tgcttcggat ctcactgcgc cgcggacgcg ggttcaccag tgacgaccgt tggagcgcgc
     3901 cacgggcggc gctggtaaac gaggcgtttg ttcgcggcta cctcgcggat tcggaggacc
     3961 cgctgcgcga ggggcttcgt ttgagcttct acaacggctt cgcgatgaag ccgtatacgc
     4021 gtttccagat agtgggcatc attgccgata cgcgcaacga cggtctgctg gtggacccgc
     4081 agccgcagat cctgattccg gcggcgcaga ttgcgatgga aggatttttc tattacgtca
     4141 agacggcgcg tccggcggct tcggtgcaaa cggagcttcg cgaggccata tggcgcgtcg
     4201 atccagcgat tgagcgcgtg accttcagcc cgttgaacga ctatgtcgag caagggctgg
     4261 ccgagcgccg cgctctgagc gcattcggct tgctggtgct gctggtagcg gcagtaattg
     4321 tggcggctgg gctcttcgca agcctttcgg cgtcattatt ggaatccacc agggaactag
     4381 ccattcgggc ggcgctgggc gcgacgccgg cgcgcctcgc ggtcgagtcg ttgcgatggg
     4441 cgttagtggc ggtgggcctc gctggagtag taactgctgt ggccgtgccg tttgtcgcgt
     4501 cgagcgtgcc attggagaag gcgatcttgc ggcccacacc agcgagcgta actctgtgtc
     4561 ttctcacaat gggcgtggta accggcgctg cggctttgcg gccggcgtgg agagctgcgt
     4621 ccgtttcgcc cgcggacgcg ctgcgtgcgc aatgagccaa gcgaaggcac tgtgcctgac
     4681 gccgcagcgt agtgggtcgc tcggtgattg gtctgaaact ctccagttgc ggcctgcgtc
     4741 caaccagtgc caggaggtgc tccatgagat tcggtttgat cttccctgtg ctgctggccg
     4801 ccgcactgaa cgccgacccg cccatgaaac cgcgcgtttt cctcgccggc gccgagccgg
     4861 agaggatttc cggcgaggct ctcctgggcg aatcccgcgg agcgctcgag ctgacccgct
     4921 ccactccgtc ctacgacatc gaatcgatgc ggacattcac ccgtcaatgc cccggtgtgg
     4981 tcgtgaccac caggcgggat aaagccgaca tcatgatccg cgtcgagcgc gacgatccca
     5041 accccaccac tcccttcgtg aaggccaaca agatcgccgt ctttaatctc aacgacgagc
     5101 tggtctacgc gacccgcgcc cggctgctca gcaacgcctg taaagacgcc tgccgtgcca
     5161 tcctcaatta cgtcaagcag tagctaagct agaccagacg gaggctggac actccggctt
     5221 gatttgctct ggcagcggcc gcaccgtgat gtgggggctg cgctggccac tgtcaccgcg
     5281 aatgcacgtt taccccgtcc cctgcgtctt atatgttca
//
LOCUS       DATIFQ010000129        39554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2724293, whole genome shotgun sequence.
ACCESSION   DATIFQ010000129 DATIFQ010000000
VERSION     DATIFQ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 39554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39554
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724293"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VLE22_05790"
     CDS             <1..768
                     /locus_tag="VLE22_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSB13950.1"
                     /translation="SRESKFYLWSDSADAPPPGPAGKDTYFMVRPTHLPGDGPGNLYD
                     SRKHEFWQYSARAGKYYWTKWGGVDKAGKRTRLSQYNWGSREFQEEAERIVRFWMDTG
                     IDGMIIDAVNWYVDYTWELGRRRITDVIASYGNVYSQPEDAGGFHEDPVPWITEGGWN
                     SVQNYALNIWWEKGSNVIQNAIETGDPRPIERALRDFRDRVVEAGGTLYFAPEARGRI
                     DDASKRRLAAAVVITTGHLVAVHEGRKGCWWTPRCAG"
     gene            744..1193
                     /locus_tag="VLE22_05795"
     CDS             744..1193
                     /locus_tag="VLE22_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13951.1"
                     /translation="MDPEVRRLIEAKRAHPALGQLARRRQLATRADDKHYAFMLAAPD
                     NSERMMVAMNFQAAPQTVEVDLSGVATPRLVEVGTGASLPRQYPARRGPACLRLPPLC
                     CQAGAGRAVTLVTTTKASSGATGFYGNFFLYPGDGGSGRLSLGRPGG"
     gene            complement(1455..2018)
                     /locus_tag="VLE22_05800"
     CDS             complement(1455..2018)
                     /locus_tag="VLE22_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13952.1"
                     /translation="MGFLCAYATALKPQLGLIFVSQIVSGLLAVICMIWLAVGLANPQ
                     AVLPQSVDPRRWHVIVIFIIVVSILNSLGAFPSKIAIGPEVTSTQTETTETSATTLEA
                     SVVCSLDSVIVSNASGEDWTEIKILVNEGYSHAVDGMTAGEAYELPQELFTNLKGERL
                     NLSRVACKTINIHATTPGGRKHCSRSY"
     gene            2124..2315
                     /locus_tag="VLE22_05805"
     CDS             2124..2315
                     /locus_tag="VLE22_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13953.1"
                     /translation="MEIRQYMAKLGKKGGKIAAFRMTKAGRAARARKGGQAGETRKPS
                     QRSSSLGETGERRHNGPSG"
     gene            2521..3357
                     /locus_tag="VLE22_05810"
     CDS             2521..3357
                     /locus_tag="VLE22_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13954.1"
                     /translation="MISLFETTQKEKTPTSREVYLKRLHEIAYDPNGKSSEPATATAP
                     DPAVEAPPETVSAETVAEMEIKTIPQLPGTLLFPAVEPFSFQGEMKVGAMEEAATLDS
                     RKLAEEIAPKFVDAFVVAFDEMERHALGSNSKASEAAAELSRASQDLQALNGQVGAIR
                     RAIDSLAAAQRELSTRVSNIEAGLREHEKINLNLEAGMKQSEQALKAQQGLLTEKLNT
                     VAEQAAPINERLDAQANAIRSLHRASPDRLGHRDDLRSTLQKIQEIAGKLSTPGSLPG
                     KL"
     gene            4245..5411
                     /locus_tag="VLE22_05815"
     CDS             4245..5411
                     /locus_tag="VLE22_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13955.1"
                     /translation="MTGGHGQNWIKTVGGVYMASEANGPSTWAGETSGTGTVEASVIN
                     IYGAGGVKEPGSYSPMPTNLDNFEPFRDPVEKATRLVGQPDAPDSQSAPLQAVSGGTI
                     FGDCANPLTVSSGTYYATDQNGVPTGQPIRVNGCVNFGTPGQFNNFVFAGGLHFADTG
                     SQVKVAPGRYFAAGAMRNNDVFYMRNGVALTDYTPLIDGNAQPTQDLGEIFVFTDQNY
                     PGLPIPADLKQQLDALGLGYGRVEVQTGNTDGSEISLHGLTTDPVLKVSLPGNLQHFS
                     PVVLYVDQGNSRIDYDNGVLQEPGDPGCIAEPCLSDTTEVDDPMMMLQSSPNYDVFGT
                     VYVPRGGSLQFQGSGNIDAPSQFIAGTFGLRGGAQINPQPLSPGFALMRAVLIE"
     gene            5607..6017
                     /locus_tag="VLE22_05820"
     CDS             5607..6017
                     /locus_tag="VLE22_05820"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB13956.1"
                     /translation="MSVRNKRASDDDRPRAKQRRTALLADLMHAQAEKPTRFPIVREV
                     RYKVMNSKGAVEVGTGETINISSKGVLFNAHAPLPSGKRIEISINWPAQLDGKCGLKL
                     VARGRIVRCEGTNVALEIEKYEFRTAGRGPGAGI"
     gene            complement(6129..6205)
                     /locus_tag="VLE22_05825"
     tRNA            complement(6129..6205)
                     /locus_tag="VLE22_05825"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6169..6171),aa:His,seq:gtg)
     gene            complement(6287..7732)
                     /locus_tag="VLE22_05830"
     CDS             complement(6287..7732)
                     /locus_tag="VLE22_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HSB13957.1"
                     /translation="MAYTDLREFIRALEKEGELKRVAVEADPVLEITEFADRAVKQGG
                     PALLFERPTGSRVPLLINAFASQRRMEIALEVSSVEEVAGRISEYLEMRMPEGLMGKL
                     KMLPKLAEMGSFFPKIVSGGPSKEVVRHEGFSLSEYPILKCWPQDGGRFITLPLVFTK
                     NPDTGKRNCGMYRMQVFDDHTTGMHWQTHKQGAEHYRRRIKEGAKRMDVAVAIGSDPA
                     TMYSAVLPLPPDLDEMMIAGFLRGKPVEMVKCETSDLEVPSNAEIVLEGYVEPGELRT
                     EGPFGDHTGFYSLEDPYPVFHVTCVTQRREPIYATTIVGPPPMEDYYMGKAIERIFLP
                     LMRLQLPEVRDISMPAEGVFHNLILVAIRKSYPLHARKVMHAIWGLGQAMFSKCIVVV
                     DEDVDVQNVREVTWKALNNIDPQRDIEFVMGPVDSLDHSSRLPNFGSKMGIDATRKWP
                     EEGFVRPWPDVIEMAPEVKRRVDELWKQAGL"
     gene            7792..8526
                     /gene="fabG"
                     /locus_tag="VLE22_05835"
     CDS             7792..8526
                     /gene="fabG"
                     /locus_tag="VLE22_05835"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HSB13958.1"
                     /translation="MVNRVAFVTGASRGIGRSIALMLCRSGYEIVVASPEVENNEAVA
                     AEIRACSGRALTLNFDLTSLDSIKSGVAAALKECGRIDVLVNNAGITKDGLAVRMKPE
                     DWNLVIQINLTGAFYTTQQVLPGMMKERWGRIVNVASVVGQMGNAGQANYVSSKAGII
                     GLTKALAREVASRNITVNAVAPGFIETDMTARLPQEVKDQMASTIALKRFGTPDDVAA
                     AVRFLASEEAGYITGNVVNVNGGMYM"
     gene            8563..11061
                     /locus_tag="VLE22_05840"
     CDS             8563..11061
                     /locus_tag="VLE22_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSB13959.1"
                     /translation="MPTIPATRRDFLRGAALGTIALGTSAQSPTRILIEEAPDTGFQR
                     RLAECELLRGLSGLGLRSEIRFATPGERPTAGDILVTLRVEPARSKNAEAYSISAARG
                     AVSLTAASDQALLYAVFDFLERQGVFFGIDGESYPLDPVSALALPTENQPWTAEPRFA
                     VRGLLPWPDFLNCITVYNQEDFRAYFSSMLRMRFNTFGMHVYTGADQWAESYLSFEFA
                     GAGHLAFLDNTASHRWGYLPQRTSRFGMGAADFYDSEVFGSDSTRLARDPWETADRAR
                     KLLQDGFSFANSLGIHTGIGFEPYQIPDEIWRALPPEVKPQELPGRNSEGPRFDIESV
                     TARKLLETRLGQLLEAYPDVEHVWLWEDEHMNWESRRTGQPFSVTPFLQAHDFLRRHA
                     PKKRLVVGGWGGVARHFDDFHKRLPMDVIFTCLSDSLGWDPVHEVFGRLEGRERWPIP
                     WLEDDPAMWLPQFHVHRFEMDLNRAAQFGCQGMIGIHWRHRIVDPTAAFQARFSWDPK
                     LAPAAYYRAYARTQAAGSRVDRLADVLTDADKNRKLLSTFTGEFKDGHAVTREFSGDY
                     GEAFAFWNDYEPDLAVAASQKEVASALRQLAGAAASDTEKERLEYLTRHVEFLVPYAE
                     AWTLAHRLHGVLKQAGESKTAGRVDEARSKVRNEGVPLWMKLAPEVRAAMLHFQRIVA
                     NRNDLGTLASMQNKFVRLALYRLPLSIQDYLGEMPPEIESLREQMLRPDSEAPARLFL
                     PTRPTLLRPGESLRIVILAPGGPPPESVALYTRVRGAREWLPVPAKLLGRRTWEATLG
                     PFRASGSLIDYYARAGRLTTPFDAPRLFYTATLP"
     gene            complement(11116..12321)
                     /locus_tag="VLE22_05845"
     CDS             complement(11116..12321)
                     /locus_tag="VLE22_05845"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HSB13960.1"
                     /translation="MDGEELSLSEARRIALVAQGFDRSRPPHGRVGARDIARTIRQLG
                     LVQIDYVNVLTPAHYLAPFSRLGPYDRKLLDGVVYGRRGFTEQWAHEASIVPVESWPL
                     LRHRMENHRVRPWGFETFLERNPDYVEWVLGEVRERGPLSADDLPEREGVSRRLEGTW
                     IGTVPRAVLEALFGRGVLAVAGRRPNFARVFDMAERVVPVEHLDRRVSREEAQRELLR
                     MAARSCGVGTAADLADYYRMPIREVRPRIEELVETGELCEVRVEGWPETAYLDAGARA
                     PGQVDAAALVAPFDPVVWHRPRAERLFGFQYRVEIFVPEEKRRWGYYVLPFLLGDGLV
                     ARVDVKADRSGRRLVVPAAYAERGVNRGEVARALASELRTMAEWLRLEAVAVGRRGDL
                     ARELQAAVK"
     gene            12422..13612
                     /locus_tag="VLE22_05850"
     CDS             12422..13612
                     /locus_tag="VLE22_05850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HSB13961.1"
                     /translation="MTFSHPKLREQAFSGDLYGTALAYVRGDVDVAGDVVAAVRFFEN
                     QARRGWRQARHNLLGRMGRWRLEPWWQSRRRAAENIRHHYDRSNDFYLQFLDRGMTYS
                     CAYFKNPEWDISRAQEAKLDLILRKLDLRPGERFLDIGCGWGSLVEEAARRGAAATGC
                     TLSPAQHEFALERLRTGSAGPTVLRTDYRDLSGQWDKIASIGMFEHVGRRRLPAYFRK
                     VYDLLSPTGLFLNHGIVRPQTAREGVESLFLRRKVFPGSELPHLGELIRASEEAGFEV
                     LDVENLRPHYALTCRAWVERLLRNEEACVRFAGRETHRTWVLYLAACAHQFETGCMDI
                     YQTLLARRSSPHGRRLTREYMYNGVSARAEVSGSEGAPRCADVTQGEPVIPIQEVKKR
                     QRQG"
     gene            complement(13517..14248)
                     /locus_tag="VLE22_05855"
     CDS             complement(13517..14248)
                     /locus_tag="VLE22_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB13962.1"
                     /translation="MHTTLKASKRARSAQKGPILRMAEMIGQSLLHFEEVVERTVAEF
                     TESTAADSRVLGVSSGEIGNTSHFKRHRYCSLELSTARNGRSQRRPHVIGDPATLPFP
                     EKRFDACVNLLTLEHVCEPGLVLRELVRTLKPGGGLLLVVQQESEINQAPHDYYRFTR
                     FGVEYLLESAGFTEIIIKPVGGLFRLLSRRLFDGLEFIPVIVFPLAALLLAPLALALP
                     LLDFLDRDNRFTLGYICTARRPLTT"
     gene            complement(14288..16099)
                     /locus_tag="VLE22_05860"
     CDS             complement(14288..16099)
                     /locus_tag="VLE22_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HSB13963.1"
                     /translation="MVSKRYSRRPKGQVLPMVALSLLTMSGVLGLALDLGWMYFVKKS
                     AQAAVDASVLAAATSVLEGVGEEGPWTCGAGMTCQEATPCPASIGTPPSSDIEVACLY
                     ASENGFRTGGRDGRQQVVMTSGVGPAVPDVSGVKVNYWVRATAAERIPQLFSAMMGNL
                     VGTSSVRATAGVVDSIVPGSLILLNRENDCLPMGNANSSVCGVNALIQANDNQGLHAL
                     QAEGGIMMASTKHGADVDARFAGENTGGGAVIAPFTYIRESGSYTLSGGATWTAQPDS
                     GKPDTRYFTDPVRGKGQPPPPRGLADRPVLGGTLSGSADPNNPLVVQPGNYYAMAINA
                     QGQPYATGDPIRLQGNLEFGDGGTGFGRYAFFGGVTNQSAGTTITFGPGSYFFVGAKP
                     QANKPAPLFDISVNMTLKDKTTGYDTPANAGEIFVFTDPNYHGGPDGTATLEIPPLVQ
                     TIASDLRHGMSGFQSGNSSSVSINLHGLNREHEALPSEMKLYAPVVLWQDQANSIIKY
                     TGRGYIDTSCGQPECPNTALWSSASTELVFQASPNLHFWGVSYQPRGSWTSMLGGGGY
                     DAPLQLITGALKVHANSNVHLRPLNLPLVRTVIALIE"
     gene            complement(16138..16668)
                     /locus_tag="VLE22_05865"
     CDS             complement(16138..16668)
                     /locus_tag="VLE22_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13964.1"
                     /translation="MFSGRSFQRRRGQVFPLVTLSLLAMSGLAVAAEFTQAKASADSG
                     GKLQVSWRETGLSPGLQYSYLVTATATATYWCVEGGTRHPTGTPRSAVNSAVWSTDAF
                     TSSKAGTVSGEISLSPPGPTEACPVGQFLVLHDVIYDEIGLRNESTGASVSIPGAYSV
                     LLFGKTIGAGKGLPIY"
     gene            16854..17879
                     /locus_tag="VLE22_05870"
     CDS             16854..17879
                     /locus_tag="VLE22_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB13965.1"
                     /translation="MDDATRRHFLEATLLSAGALDSCSPSASVSAPRTMERRDFEPAY
                     LRLERTGDLARRARELYSIYRACRLCPRACGVNRLKGEKGVCSSTSRAKVYSAHSHFG
                     EERPLVGRGGSGTIFFSNCNLLCVFCQNWKINHRGEGSYVSDDQIGRLMIDLQDIGCH
                     NINLVTPTHIVPNIVQGLRSAVARGLRIPIVYNCGGYEPVEILKLLDGIVDIYLPDYK
                     YTDGRMSEKYSSGARDYPEVAAAAIEEMHRQVEELVVDENGVALRGLIIRHLVLPQNI
                     AGTDKFVRFVANELTPSTYVNIMAQYRPEHLAHKYPELSRRLTRAEYQQAVNWARAAG
                     LTHITGA"
     gene            17954..18295
                     /locus_tag="VLE22_05875"
     CDS             17954..18295
                     /locus_tag="VLE22_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSB13966.1"
                     /translation="MTETQVKVTRDLCMGLIRLHVLHHAAEEPIFGLGIMEELARHGY
                     RLSAGTLYPILHGLERRGYLRSRVERRGRVNRRVYRATPLGRRALSMAKRKVQELFGE
                     LFEETTHDLLS"
     gene            18279..19757
                     /locus_tag="VLE22_05880"
     CDS             18279..19757
                     /locus_tag="VLE22_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB13967.1"
                     /translation="MTCCLDQNRQQNRGLAPISPCLRNWWLPPRFAWHPVLPLTFYLT
                     LLLALGGSLACAEQPLTIGQVVATAGEQYPSIRVSEERISSAAAAVSLARTSYLPRLD
                     FLAQANRATRNNVFGMLLPQSIIPSISGPPSMTNSLTSVWGSTVGLLATWEPIDFGVR
                     AAKVRTADAGRLRAEAARGRTRFEVSALAADAFLTVLAAQQTLVSAQAGVERSGTVVK
                     VVKSLVDSELRPGADLSRAQAELALAETQVIQSRSAIRVAKASLAQFTGGDPGAMTLI
                     AGKLLDLPASKPLPGESAAQHPAAAEQAAASAEAAAKLRELDRSYYPKFLLQGASYAR
                     GTGAIPDGTALGGVNGLGPNIHNWGVGFTATFPILDLPAIRSRRQEEQHLLRAEQARE
                     EQVRRELDAGLQRSNAALEGAWEVAAKTPEQVAAAHATLEQASARYRAGLGTIVDVAE
                     AQRLLTQAETDDSLARLQVWRAMLAGAVAQGDLGPFLQTAAQ"
     gene            19770..22919
                     /locus_tag="VLE22_05885"
     CDS             19770..22919
                     /locus_tag="VLE22_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB13968.1"
                     /translation="MWLVNTALRRPITVLVAILAVVLSSWLAVRRMRADIFPSLGAPT
                     IYVAQPYGGMDPSQMEGFLTYYYEYHFLYITGIEHVESKSIQGVALMKLQFHPDTNMN
                     QALSETVAQVNRARAFMPPGAVPPFIVRFDAGSVPVGQLVFSSPIRSPGEMQDIAINR
                     VRPLFATLPGVSAPPPFGGNQRTIVVRLDPDRLRGYGVSPEEAIQAVSKASTVLPSGS
                     VRIGDQTIIASTNVVIGGDLNELTGAPVRTGNGPAVYIRDIGVVENGADIITGYAHVN
                     GRRTVYIPVTKRSDASTLAVIREVKEALPAMKNAVPEDVDVRLEFDQSGYVARALRGL
                     VNEGLLGALLTGLMVLVFLRDWRSALIVVATIPAALLAAVVWLWAAGQTINIMTLAGL
                     ALAVGVLVDEATVEIENIHTHMATGASRAWAVLDACRKTALPRLLSMLCILSVFVPSF
                     FMAGVGRQLFIPLSLAVAFAMVSSYFLSSTLVPILSTWLMRRSQGSDEDRGFFGALRA
                     LYARYLGFVIRFRWPLVAAYSLSVALLIWLMFPRLGTEIFPVVDTGQFQLRLRAPTGT
                     RIERTEIMTLKALDLITREVGPDNVRISTSFIGVQPASYPINTIYLWTSGPHESVLLV
                     ALKPAAKLRGDALRERLRARFAQELPNVKVSFEAGDIVSQVMSFGSPTAVEVAVQGPS
                     LPANRAYAEKVYAELAKLRSLRDVQYSQALDYPTLQVNVNRDRAGQYGLTAASVAKSL
                     VAATSSSRFIEPNYWRDPNSGNAFQIQVEIPQHRMASLDDVRSIPLARNGGTPVELAD
                     VAELKMANSMGLVERYNMQRVVSITANIHGRTLGEIAGELRQALKRVGNPPRGIAVGV
                     RGQIPPLEETLGGLRIGLLLSIGVIFLLLAANFQSFRLALAVIAVIPAVLCGVLLILL
                     VTGTTLNVQSFMGAIMAIGISVANSILLLSFAEMSRADGAEPGRAAVEAGRGRLRAIL
                     MTATAMIAGMIPIALGLGEGSEQTAPLGRAVIGGLAAATFATLTILPAFYAVLQRRAT
                     AHSASLHPMDPRSRFYEVR"
     gene            22906..23979
                     /locus_tag="VLE22_05890"
     CDS             22906..23979
                     /locus_tag="VLE22_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB13969.1"
                     /translation="MKSVSVLVFALASLCFGQPADLVAVASRPLERTVKLPGEFAPYE
                     QVDLHAKVSGYVDKVLVDRGSVVKEGDLLVTMSAPEVKAQVAEADAKVKAIEAQMAEA
                     RARILAAQSTYDRLKQASSTPGAIAGNELVIARETVEAAKAALMAQEGARDAARASAD
                     TLRELQKYLDIRAPFSGVITERLVHPGALAAAGTGAAAPLLRLERLSSLRLTVAVPES
                     AVGGSLPRGQVEFQVPAFPGRSFRGTIARAARTLDPNTRTMPVEIDVQNSNGLLAPGM
                     YPEVLWPARGLGASLLVPPASIVTTTERTFVIRSTQGKAEWVTIRRGAAAGDLVEVYG
                     PLKPGDRILKRATDEIREGTKLP"
     gene            complement(23985..25193)
                     /locus_tag="VLE22_05895"
     CDS             complement(23985..25193)
                     /locus_tag="VLE22_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB13970.1"
                     /translation="MAPTVMVILALLTLQAPVEVPGVDWKAEIEKGNKAIAARDYAEG
                     TARFRSALAQLETAPGEDAGVLAAIRGCATGSRLQGHGADAEQYLSRAVVVAVRLHGD
                     ASLEVAALLSELAAVQRSNGRRQEALTTLQSAVRIRQSRTDGKAEDLARDITGVAILQ
                     VALGDAKFAEETLKQALTAWEAAVAPDSPQLLPVLDALGGIHQTNADYEKAEPLYLRA
                     LRVREAALGPDSSELLSTLDSLAYVYFGQKKFSEAEPVYKRLLALWESSAGLEHPMVA
                     LTLDKMAEFYAFQQRYEEAEKAASAALTIRTTAHIASLNQVGRVLLMEAKLGEAEDLY
                     HRAIGIGDLAKAPDEMLDPLLRIYAKVLRAGNRLREADTVDKRIKDALLRKADREGRR
                     PSPVKPPVGQ"
     gene            25442..27826
                     /locus_tag="VLE22_05900"
     CDS             25442..27826
                     /locus_tag="VLE22_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB13971.1"
                     /translation="MRKDLLYAVRNMARNPGVSVVAVLTLVLGIGANTAMFSVIHAVL
                     LRPLPLREPERLVTISAGIPSMNIRNASVEYNTFTEWWRARTRSFEAMSAYTPETANL
                     TSGDQPQRVRMLRVSANHFSVVGTRPAAGRDFLDEEDRPGAPRVSIISHGLWERRFGG
                     DRALLGRAIVLDGKNYTVVGILPSGFDLNPEDVFTPIAHSGARVPDMPTVGAYARLKR
                     GVAVETAQAEIDGLCRGWVAQYRYPKDWGARVWPIREFMVRDVRASVLVLAVAVALVL
                     LIACANVSNLLLARAGDRQREIAIRTALGAGRKRIVRQLLTESALLGTVAAGLGLALA
                     WAAVRALAAVELPFPFLRKVSIDAPVLLFTTAAMLLTTILFGLAPAVAGAQTGLVENL
                     KEGGRGAGESIRRSRFRSALVVAEVALALLLVIGATLTVRSLARLMAVDPGFNPEGVL
                     TAQLTLPQSSYAEPVRRVNFFKTVLERLAATPGVKAAGMVSHLPFGGSKSGLDVVPEG
                     APPVRPGEQKIAFARATDPEYFRVMQVRLVRGRFFTGRDPAGPPVAVINETMARRCWP
                     GQDPVGKRFGLGRTDFRLTVVGVSADMRNTSLADEPDAEYFAPYAQSPGASMALVVRT
                     ELDPMSVASAIQAVVRELDKDLPVSEVATMSAKISQSTSTRRFSVALLGIFALIGLML
                     GAVGIYGVVSYSVSRRTREIGLRMALGAAGGRISAMVVGRAVLLAACGVAIGIAGSLA
                     LTRLLGSMLFGVSATDPVVFAAASVFLVALSALAAYIPARRASRVDPLVALRHE"
     gene            27970..28074
                     /locus_tag="VLE22_05905"
     CDS             27970..28074
                     /locus_tag="VLE22_05905"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSB13972.1"
                     /translation="MEQLVYHLWEESGRPTGKAEEHWYRAEQLIHGAA"
     gene            complement(28098..29024)
                     /locus_tag="VLE22_05910"
     CDS             complement(28098..29024)
                     /locus_tag="VLE22_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13973.1"
                     /translation="MTSHLRKTHSIYLGILTILLCNVANAQSTHPKDFVTVKERPPRG
                     QDLTLNPGDSEVDERGKLVIDFTEAKMQPKDEGGRPRVIIRISGELFKEKSSLPLVPI
                     QYYVSKGNEQNPVVVHDKDFLNPGDIMPDTDINLRSIGFGDYEYLELRITNLVTLESV
                     VYTVSPQPFGFRVKVTDSLLFLKRLGVSEEDKKNGVDAINFGPSPGVTYGGTYLSRGN
                     DFLRFMRPGAGMNVSFMNWKDPAFDLSTGKFAPATKGSDVQVGLGVQFSLFNNILQFT
                     YGANLHVEQDRQYWGIGISFVNLTSKVASLIQ"
     gene            complement(29040..29312)
                     /locus_tag="VLE22_05915"
     CDS             complement(29040..29312)
                     /locus_tag="VLE22_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13974.1"
                     /translation="MNKFLIFFKSGQDQLSRVFDIVDQAEACRSATGKSFNIRHVDVS
                     DAGDPDLPHWSGQRTIECVTVVNNAVFDRRVSPPPVDIGVMVRNLP"
     gene            29537..30013
                     /locus_tag="VLE22_05920"
     CDS             29537..30013
                     /locus_tag="VLE22_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSB13975.1"
                     /translation="MNHVTRRRWLQTSMLLAGAPCACLAKASNDCCTVPEAPGDAVRI
                     QNGLLTIDLTLTPGLKHIGSAVKVVDPARKLRVIIARPGKHQFVALDQKCTHGGGALT
                     YVHRHKHLYCTCWGHSKFALDGSVLRWPNKLTPRPLRAHPVERKGNLLEIRVEGLA"
     gene            30010..30768
                     /locus_tag="VLE22_05925"
     CDS             30010..30768
                     /locus_tag="VLE22_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HSB13976.1"
                     /translation="MIHLLLAVACGVMAFAQKPEFVEIPAGSFIMGCDPAWKCAQSLP
                     KKRVDFDRPFLMSKTEVTVGQFRAFVKATSYRTDAEKAGEARNWQSPGFPLSNKQPVV
                     FLSLNDAKAYCEFIGARVPFEAEWEYAARAGSTTHHYWGEEIDGRYLWYFNNTDERPM
                     PVGRKLPNAWGLYDVEGNAMEWVRDGGPHSSITRQGAGSVRGASYASCPEPYPPMDGL
                     RQRAIGLGPTFPGFENSNFRPDERRFDYGVRCAK"
     gene            complement(30842..33178)
                     /locus_tag="VLE22_05930"
     CDS             complement(30842..33178)
                     /locus_tag="VLE22_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB13977.1"
                     /translation="MRCVRHLAVLLPILLLAAGCARRDQGSHKQEAPYQPYRFDDNLE
                     TAATVSAASGQTAARVTDPVVWKDFFTEKDATWELVRGRMGLRDGDLILKGDGESPVI
                     VSPKEFAIDWSLFESVQIRMMAEGGSEVRIRIGPLDMRQKLAPPKQYQVYRFAVDIQA
                     PTGSRPLAVIPTDSPFHLAAIDFIELVPRRTSFPKAAGRQVLGKGEEYRNTIYAHSPS
                     AITYEAPIPEKGRLKFGIGVAEKKPVTFRVLAGPSQSPVFSKTLEDPERWADAEVDLS
                     QYAGRTTKIIFETSSESQGAIGLWANPLLTTAAAKARPNVLVYLVCSMRADHTSLYGY
                     SRDTTPFMKQLGAAGVVFDDAQAQAPWTKGSVPSLLTSIHSYTLGMRQDTDAIPQRAV
                     TLAEALRAAGYSTVGIVTNPYAGRASGLNRGYDYMMEYPVVHRLRTDEADRGTDSAAV
                     NKVAMPWFEQHRDEPFFMYVHTTDPHAPYRPPKEFEERFANPAETEQFNRDYQNLWDK
                     RQYGGGMAVTRAKCKAKGIDPDKFLRRAIDRYDGEIAHADRSLEVLTGKLKELGILNN
                     TLVVVLSDHGEEFWEHGLTGHGHSLYQELVHAVFLMWNPKLLPAPRRVSDTVQLLDVY
                     PTILDLAGVAPQGVMQGQSLAPLAKGQPFQRKGPVMSSRFALPFAKPGGLVPENCTDT
                     YAIWNGPWKLLYRDRARLAGLPEVELYDRRTDRKETTNVASRNSGVAQKLRAEIDQWL
                     KAQDQIRKLLGPGGETTLDRQSIERLRSLGYLGGKPSQ"
     gene            complement(33318..36902)
                     /locus_tag="VLE22_05935"
     CDS             complement(33318..36902)
                     /locus_tag="VLE22_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB13978.1"
                     /translation="MLILTLVLVVVPVMGQTGLGVVRGTVQDATKAVIPNAKVILTHT
                     ATGVARAAETNAVGIYYFASVPIGPYTLAVEASGFKKWAGTLRVEVGQTVVVDTTMEV
                     GALEATVEVTGAAPVVTTEGMEVNDVKDRLRIEQLPLNGRVVSNLFDLTPGVEGGGSP
                     RINGMKVGSADMLLDGISIMDRFGGGMRPVQPGLDIIQEYRVETTGSGAQYSRPATVT
                     LVTKSGTNELHGSVFWTHRNNFGGLRARQRQDFFSRPPQYIRNEFGASAGGAIIKNKT
                     FWFAAYEGQRERQARYARTQAPTDAIWGGDFSTAVDYDNTPITIYNPFSTMADGTRLP
                     FNGNRIPSNMIHPFASVMKSVSAAPAGPNAGGNAWIEANFETYYPVTLDITTYTVKGD
                     HVFSEKDNLSGRFTHGTRPRKVYGGQYGYPPPGMSNAGGTGRSDTTIYSAFSRWNHVF
                     TPTFLNEFQASANRNANSSGTLADSTNWADKLGLPNPFGETGWPTMCAGPFFDWCWDA
                     DNPQKQNLTAYQIEDNVTWIRGTHSVKLGFKGRSEYNNARELQQAQGMHYFWGDWTSL
                     YDPAAQQAVSYTGTGFGSILLGLPTYLSNQYNRGFFYFQQKEIGLYVQDSWKLSPRLT
                     VDLGLRWDKWTVYKEKYNRLVNLDIQNYVGKMEVVTPHDTRMEDIPGIPPAVLASWAK
                     RGVTWKTANEAGLPGSLLPADNNNFAPRVGAAFRMSDKWVLRGGYGVYYWTMPLSQIL
                     QSARVSAPLNLRFENDIATQNGSIYNYALKSVPAATDIVGKAQVNIEGVAGISSQAQP
                     FMPWDFRTWSDNMMQEWNLTFERELMKETALRLSYIGNHGSDLEQRWRWNDGESQYNY
                     QLRTGLAAPSNEDLRRANPNWTSGCCNAPLRHNGFSNTHSLQAELERRYSNGLAFQWF
                     YTFSRVMTTTDTGGFNFGSSGINNSGSGTAFAAPENHVIMGAPALSEDQRLRLGYANS
                     DSVPAHRIRWNGIYDLPFGKGEKFGNGASGALNHVIGGWQIAFIGDWRSGFWTGVGSG
                     RYLFGDPTLSADERLLLTYAGRPRRLWFKGDFDTSLASNVDASKLAALIPADRSQRVL
                     RPVGGGFDNRIPMELADGTVRATGIGDMLNWNARNFFFGPGSWNTDISLFKTILVTER
                     IKTKITADFFNALNHPVDRPPDGTTGLQDLSQQLNDPRIIQFSLRVEF"
     gene            37179..39116
                     /locus_tag="VLE22_05940"
     CDS             37179..39116
                     /locus_tag="VLE22_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB13979.1"
                     /translation="MRLDSISHRSGRRWWWAFALLIATPALALALLGLRVAQLESLQR
                     AQQTREEQSQLGRLTDTTISSMLTVLKSELARTETSLAAARDYTPFVLEPGGRLVFPR
                     EKTYFPDPSVLDSHSGPQWSQATSQEIERAQIADQQRAGEASSLFHHIGKTEPRLRAW
                     SELALVRRQFESGDVSALTALANPSWTHSEGMTPSGLPVALLAATYAEGLPPDQRSRF
                     VPLLKATLEALRAGRWWFSIEERRFYDEELRRLIALAGSRPPADDARLAEWASIERLA
                     RRIRPAGDARPSDPDPAAPFLLLWSHSPDGTWLGAAVSRDRLTGLVDRALQPLFTTRS
                     FAVAIRHLKGPIIWSRPLQSTLAGAAAPLQAVEDWELVFSRPTEGGAFDRRRLLWYGF
                     TLVVVMTMAVGLAVTLFVVKREVELNQLQSDFVAAVTHEFKSPITSIRLLVERLSSGR
                     LRTPAAAGEYQDAIHRETDRLERLVNRVLESQKIQSGEKRYNLVPGSVVELAEAAIWR
                     LRPQADAKNIGVDLELAGEIPQIQIDKTAVADALDNLVDNAIKYSPEGARVLVRIQRR
                     ERFLCVEVQDHGIGIDPEDLPRIFDRFYRGRRGDQHDVHGTGLGLALVKAAAEGHGGS
                     VEVTSAPGQGARFCLRLPLES"
     gene            39116..>39554
                     /locus_tag="VLE22_05945"
     CDS             39116..>39554
                     /locus_tag="VLE22_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB13980.1"
                     /translation="MAPRILVVDDEPSIILAVKDEMEFEGFVVDSAADGPSGLKKARE
                     SRPDVLLVDLMLPGLNGFEICRQLRADLPDLWIIVLTARGQEVDRVTGFEAGADDYIV
                     KPFSLRELVGRVKVGLRRRKATAPALLRFGDLVIDPGAHRVTKA"
BASE COUNT         7280 a        12785 c        11772 g         7717 t
ORIGIN      
        1 agccgggagt caaaattcta tctttggagt gacagcgcgg acgcgccgcc tccgggtcct
       61 gccggcaagg acacatactt tatggtccgc ccgacccatt tgccggggga cgggccgggt
      121 aacctctacg actccaggaa gcacgaattt tggcagtaca gcgcgcgagc cggcaagtac
      181 tactggacaa agtggggcgg cgttgacaag gccggcaaac ggacccgcct gtcgcagtac
      241 aactggggga gccgcgagtt ccaggaagag gccgagagaa tcgtccgctt ctggatggac
      301 actggtatcg atggcatgat catcgacgcg gtgaactggt acgtggatta cacctgggaa
      361 ctcggacgcc ggcgcatcac ggacgtgatc gcgagttacg ggaacgtata ctcccagccc
      421 gaagacgccg gcggctttca cgaggacccg gtgccctgga tcaccgaggg cggctggaac
      481 tccgtacaga actacgcgct gaacatctgg tgggagaagg gctctaacgt aatccaaaac
      541 gctatcgaga cgggcgaccc gcgccccatc gaacgggcgt tgcgtgattt ccgcgaccgc
      601 gttgtcgagg cgggcgggac cttgtatttc gcgcccgagg cgcgtggccg catcgacgat
      661 gccagcaagc ggaggctggc ggcggcggtg gtcatcacga cgggccacct ggtggcggtt
      721 cacgaaggcc ggaagggctg ttggtggacc ccgaggtgcg ccggctgatc gaagcaaagc
      781 gggcgcatcc ggctctgggg cagctcgcac ggcgacggca gcttgcgaca cgcgctgacg
      841 acaagcacta cgcgttcatg ctcgcggcgc cagacaactc cgagcgcatg atggtagcga
      901 tgaactttca ggcggcgccg caaaccgtcg aagtggatct tagcggtgtc gcgacgccgc
      961 ggttggtgga ggtcgggacc ggtgcttcgc tcccccggca atacccggct cgccgtggac
     1021 ctgcctgctt acggctaccg cctctttgtt gtcaagccgg tgcaggccgc gccgtgaccc
     1081 tcgtaactac gacgaaagcg tcctcagggg ccaccgggtt ctacggcaac ttctttctct
     1141 atccggggga cggtggtagc gggcggcttt cgctggggcg acctggcggt tgaccaaaaa
     1201 aaggccaccg gcagcgccga tggcctggag tgtcagagaa gagaacgctt cctcgcgtct
     1261 actgttctcg gcaatgatgc tcctgtcccc tttttctgtc aagtccattc tggacatcga
     1321 agaatacggc aaagacgatg gggcagtggc ggcgacacaa aggccaccga accggtgaga
     1381 ttcgatgacc ctgtgggtcg acgtggcgtt tgaggcttgt gtggcccgtt tgaccggata
     1441 gcattcttct cgttttaata cgaccggcta caatgcttcc ttccaccggg agttgtcgca
     1501 tggatgttga tcgtcttaca ggcaacgcgg ctgagattca agcgctcacc cttcaagttt
     1561 gtaaaaagtt cctgggggag ttcataagcc tctccagctg tcatcccatc cactgcgtgg
     1621 ctatatccct cattcacgag gatcttgatc tcagtccagt cttccccact cgcattagag
     1681 actattacac tgtcaagcga acatactacc gatgcttcca gagttgtggc gctagtctct
     1741 gttgtttctg tttgggtgga agtcacttct ggtccgatgg ctattttcga gggaaaggcg
     1801 ccaagggaat tcaagatcga caccacaatg atgaaaatca cgataacgtg ccaacgtctc
     1861 ggatcaacag actggggaag taccgcttgt gggttagcca gaccgacggc aagccagatc
     1921 atacagatca ccgcaaggag gccagacacg atctgtgaga cgaagatcag ccccagttgc
     1981 ggcttcaggg cggtggcata tgcacagaga aaccccactg tcgcttcctc tcgcgtgcca
     2041 atatatcacg cgcccgacaa cggctccagg ctcagcccaa ctggggaaaa tcgactccac
     2101 agcgagacag tcgggagctc tcaatggaga ttcgacagta catggcgaag ttggggaaga
     2161 agggcggcaa gatcgccgca ttccgcatga ccaaagccgg acgggccgct cgagccagaa
     2221 agggcggtca ggctggcgag acacggaagc cgtcgcagcg aagctcatca ctgggagaga
     2281 ccggggagcg ccgacataac gggccaagcg gctgacgatt acccctgcag atgggacagt
     2341 attgctggta gaagagaccg cttgattgca gccgttagag gctggcgggg tgcaattgca
     2401 tcaccgagta aaccaaagtt agtatccgtt gtagacccgg aggaaacatt gacagaaaat
     2461 ggcttcggct ctacactcgt cctgaccctg gattcgacta gccggtaaga gagggcgacc
     2521 atgatcagtt tgtttgagac gacgcaaaag gaaaaaacgc caacatcccg cgaggtctat
     2581 ttgaagcgcc tccatgagat tgcttacgat ccaaacggga aatcgagcga gccggcaaca
     2641 gcaaccgcac cagatcccgc ggtagaggcg cctccggaga ccgtctccgc cgagaccgta
     2701 gcagagatgg aaatcaagac aatcccacag ctgcccggaa cattgctttt tcccgcggtc
     2761 gagccgtttt ccttccaggg cgagatgaaa gtgggggcca tggaggaagc tgcgacattg
     2821 gattcgcgga aactggcaga ggagatcgcg ccgaaattcg tcgacgcgtt cgtcgtcgca
     2881 ttcgacgaga tggagcgtca cgccttgggt tcgaacagca aggcttccga ggcggctgcc
     2941 gaactgagcc gggcgtcgca ggatctgcag gcgctcaacg ggcaggtggg ggcaatccgc
     3001 cgggccatcg attccctcgc cgccgcccag cgagagcttt ccacgcgagt gtcgaacatc
     3061 gaagccggcc tgcgtgagca cgagaagatc aacctgaacc tcgaggccgg catgaagcag
     3121 tccgaacagg ctctcaaagc acagcagggc ctcctcaccg aaaaactgaa cactgtcgca
     3181 gagcaggcgg ctcctatcaa tgaacggcta gacgcccagg ccaatgcgat ccgcagtctc
     3241 catcgcgcat ccccggatcg cctaggccac cgcgatgatc ttcgctccac tcttcaaaag
     3301 attcaggaaa tcgcaggcaa gctgtccaca ccgggttcac tgccgggcaa actctaagtc
     3361 ttgggtgacc ttccttccag gtccctgtta tcagacaatc cttcgtgaca aacttgccac
     3421 taaccatcgt taatggtcgt actatacact tccgctctga gtggcctggc actggaagtc
     3481 tcgcctgtag aagtgtggat cttgactaca gacaagtacc atcaacgtta tgaaagataa
     3541 cgtaatgttg cactagcaca aaagtttgcc acagatttgt ccaaaagata gccaccacaa
     3601 cttgtaactt ctgcactact cttatggttg gtaaggagga ccagcgtatg cggctggtgt
     3661 ccggtggaac gcgtcgtggt caggttgtgg taatggtcgc ggtgggcctg atcaccatgt
     3721 gcggtatgtt gggcctggtt gttgatcttg gctggtcgta tttcgtcaag aaatcggcac
     3781 aaggagccgc cgatgcagcg gcactggcag cggctttggc ggcgtacgat accgctggtc
     3841 cctctgcgcc atataacaac aaatgcggag tgagcttgga gtgttccccg acaccggttc
     3901 tttgtcccgc gtctgacaat ctgatggccg gatgcctgta cgcacagcag aatggttttg
     3961 cggtggatgg acacgggggt cgtcaagctg tgaggatggc cgccaacgct gagttcccgc
     4021 cagcttctcc gccgaccgcc cccggcgtcc cagtttacta ttgggtcact gctcgcaccg
     4081 tggaagatgt cccacagctc ttctctgctg tcctgggaaa ctcatcggga gttgtgtccg
     4141 cacgggccac ggcgggcatc gccagtattc cgtttcccgg tgtcatttac gggctcaata
     4201 tcattacgga tcctggtcca tttggaaccg gggtggatat ttcaatgacg ggcggtcacg
     4261 gtcaaaactg gatcaaaacg gtgggcgggg tttacatggc ctctgaggcg aacggcccca
     4321 gcacctgggc gggcgagacg agcggcacgg ggactgtgga agcctcagtg atcaacatat
     4381 acggagccgg cggcgtgaag gaaccgggct cgtacagccc gatgccgacc aacctggaca
     4441 attttgaacc ttttcgcgat ccggtggaga aggcaacgcg cctggtgggt cagcctgacg
     4501 ccccggattc acagagtgcc ccgttgcagg ccgtctctgg cggaactatc tttggcgact
     4561 gcgcgaatcc gctcacggtc agctccggta cttactacgc cacagaccag aacggggtgc
     4621 cgactggcca gcccatccgg gtcaatggat gtgtgaactt cggtaccccc ggacagttca
     4681 acaattttgt cttcgctggc ggcctgcact ttgctgatac gggatcacag gtgaaggtcg
     4741 ctccgggccg ctatttcgcc gcgggtgcca tgagaaacaa cgatgttttc tacatgcgca
     4801 atggggttgc tttgacggac tacacacctc tcatcgacgg aaacgcacag ccgacccagg
     4861 accttggtga aatcttcgtg ttcaccgacc agaattatcc cggccttcca attcctgcgg
     4921 acctcaaaca gcagctggac gctctgggtc tcggctacgg aagagtcgaa gtgcaaactg
     4981 gaaacaccga cggctctgaa attagtctcc atggtctgac gactgatccg gtgctaaagg
     5041 tatcccttcc aggcaacctg cagcacttct cgccggttgt cttgtacgtc gatcagggaa
     5101 attcgagaat cgactacgac aacggcgtgc tccaagaacc tggcgatccc ggatgcatcg
     5161 cagaaccctg cctgagcgac acgaccgaag tggacgatcc gatgatgatg cttcagtcga
     5221 gccccaatta cgacgtattc gggaccgtgt acgtgccgcg cggcggtagc ttacaattcc
     5281 agggctctgg gaacattgac gcgccgtcgc aattcatcgc cggtacgttc ggtctgcggg
     5341 gcggtgcgca gatcaatccc caaccgctca gtcccggatt cgcactgatg agagcggtgc
     5401 tgatcgaata agaaaatgtt cgtcggagag cagcgtcacc cagcctgaca agatgagggt
     5461 tgtttcttcc cactagggga agaaactcct gtgggtgaag cagcaacggg tcgatctctc
     5521 cacacattct catcggttta acggcgttct catttggcta ctgacgtgcc acaactggtc
     5581 ttgtcgtcac caacgggggc gcgaccatga gcgtgcggaa caagcgtgca tccgatgacg
     5641 accgtccccg ggccaagcag cgccgcactg ccttgctagc agacctgatg catgcgcagg
     5701 cggaaaagcc gactcggttt ccgatcgtcc gtgaagtccg ttacaaagtg atgaattcga
     5761 agggagccgt cgaagtcggt accggcgaga caatcaacat cagcagtaag ggggtgttgt
     5821 tcaacgcgca cgcacctttg ccttcgggca agcggataga gatttccatc aattggccgg
     5881 ctcaactcga cgggaaatgc ggcctgaaac tggtggctcg tggccgcatc gtgcgttgtg
     5941 agggcacaaa cgtcgccctg gagatcgaga agtacgaatt ccggaccgcc ggccgaggcc
     6001 cgggagcggg aatctgagtt cgtggcctat caggtcggct gccggagcgg tgaacgatct
     6061 acggcaaagt aacgggcaga gtcgcccctc ggcgaacttg cttatttcca gtaaattctc
     6121 gaattgaatg gggtgggcgg agggattcga accctcggcc accggaacca caatccggcg
     6181 ctctaccaag ctgagctacg cccaccgtgg ggaaacttca ttttagcaaa tccacggaaa
     6241 aaccaggaac agcccgcatc cgcgttttct cgtcagctcc cggcttctac aaccccgcct
     6301 gcttccacag ttcgtccacc cgccgcttca cctccggcgc catctcgatg acgtccggcc
     6361 atggccgcac gaatccctcc tccggccact tccgcgtcgc gtcgatcccc atcttcgacc
     6421 cgaagttcgg cagccggctc gagtgatcca gcgaatccac cgggcccatc acgaactcga
     6481 tgtcccgctg cggatcgatg ttgttcagcg ccttccaggt cacctcacgc acgttctgca
     6541 cgtctacgtc ctcgtccacc acaacaatgc acttcgagaa catcgcctgc cccagccccc
     6601 agatcgcgtg catcaccttc cgcgcgtgca gcgggtacga tttccggatc gctaccagga
     6661 tcaaattgtg gaacaccccc tccgccggca tcgagatgtc ccgcacctcg ggcagttgca
     6721 gccgcatcag cggcaggaag atccgctcga tcgccttgcc catgtagtag tcctccatcg
     6781 gcggcggccc gacgatcgtt gtcgcgtaga tcggctccct ccgttgcgtg acgcaggtga
     6841 cgtgaaacac cggatacggg tcctcgagcg aatagaatcc cgtgtggtcg ccgaacggac
     6901 cctccgtccg cagctccccc ggctccacgt atccttcgag aacgatctcc gcattcgacg
     6961 gcacttccag gtccgacgtc tcgcacttca ccatctccac cggcttgccg cgcagaaagc
     7021 ccgcgatcat catctcgtcc aggtccggcg gcagcggcag gaccgctgaa tacatcgtcg
     7081 ccgggtcgct cccgatcgcc accgccacgt ccatccgctt cgcgccttct tttatccgcc
     7141 gccggtagtg ttccgcgccc tgcttgtgcg tctgccagtg catgccggtc gtgtgatcgt
     7201 cgaacacctg catcctgtac atgccgcagt tgcgcttccc ggtgtccggg ttcttcgtga
     7261 ataccagcgg cagcgtgatg aaacgcccgc cgtcctgcgg ccagcacttc aaaatcggat
     7321 actcggacag cgagaagccc tcgtgcctta ccacctcctt cgacggcccg cccgagacga
     7381 tcttcggaaa gaagctgccc atctccgcca gcttcggcag catcttcagc ttccccatca
     7441 gcccctcggg catccgcatc tcgaggtact ccgagatccg ccccgccacc tcctctaccg
     7501 atgacacctc gagcgcgatc tccatccgcc gttggctggc aaaggcattg atcaacagcg
     7561 gaaccctgga acccgttggc ctctcaaaca gcagcgccgg gccgccctgc ttcaccgccc
     7621 ggtcggcgaa ctcggtgatt tcgagcaccg gatcggcttc caccgccacc cgctttagct
     7681 ctccctcttt ctccagcgca cgaatgaact cccgcagatc cgtgtaagcc atatgggtgt
     7741 gttccctttc tactatgatt gatgggttat agccaacatc caggagattt catggtgaac
     7801 cgtgttgcct tcgttaccgg tgcgagccgt ggcatcggcc gctccatcgc tctcatgctt
     7861 tgccgcagcg gctacgagat cgtcgtcgcc tctcccgagg tcgagaacaa tgaggcggtg
     7921 gccgccgaga tccgcgcgtg cagcgggcgc gcccttaccc tcaacttcga cctcacctcg
     7981 cttgactcga tcaagtcggg cgtagccgcc gccctcaagg aatgtggccg aatcgacgtg
     8041 ctcgtgaaca acgcaggcat caccaaggac gggctcgccg tccgcatgaa gcccgaggac
     8101 tggaacctcg tcatccagat caacttgacc ggcgcattct acacaaccca gcaagtcctc
     8161 ccaggcatga tgaaagagcg ctggggacgc atcgtcaacg tcgcctccgt ggtcggccag
     8221 atgggcaatg caggccaggc caactacgtc agctccaagg ctggcatcat tggtcttacc
     8281 aaggcgctcg cccgcgaggt cgcgagccgc aacatcaccg taaacgccgt cgctcccggc
     8341 ttcatcgaaa ccgacatgac cgccaggctg ccccaggaag tgaaggacca gatggcctcc
     8401 accatcgccc tgaaacgctt cggcacgccc gacgatgtcg ccgccgccgt ccgcttcctg
     8461 gcgagcgagg aagccggcta cattaccggc aacgtcgtca acgtcaacgg cggcatgtac
     8521 atgtaaggaa ccgcgagcgc aagcgagcgg ccggaggtgc ctatgccaac catcccagct
     8581 acccgtcgtg acttcctccg aggcgccgcc ctcgggacga ttgccctcgg cacttccgcc
     8641 cagtcgccga cgcgcatcct catcgaggag gctcccgaca ccggcttcca gcgccgcctc
     8701 gccgaatgcg agttgctccg aggcctgtcc ggccttggcc tgcgctccga aattcggttc
     8761 gctactcccg gcgagcgccc caccgccggc gacattctcg tcacgctccg tgtcgagcct
     8821 gcccgctcca agaacgccga ggcttactcc atctccgccg cccgcggcgc cgtctccctc
     8881 accgctgcga gcgatcaggc cctcctttac gccgtcttcg acttcctcga gcgccagggc
     8941 gtcttctttg gcatcgatgg cgagagctat ccgctcgatc ccgtctccgc gctcgccctg
     9001 cctacggaaa atcagccctg gaccgccgag ccccgcttcg ccgtccgcgg cctcctcccc
     9061 tggcccgact tcctgaactg catcaccgtc tacaaccagg aggatttccg cgcctacttc
     9121 tcctccatgc tccgcatgcg cttcaacacc tttggcatgc acgtctacac cggggccgat
     9181 cagtgggccg agtcgtacct gtcgttcgag ttcgccggcg ccggacacct cgccttcctc
     9241 gataacaccg cctcccaccg gtggggctac ctcccccagc gcacgtcccg ttttggaatg
     9301 ggcgccgccg acttctatga cagtgaggtc ttcggctcgg actccacccg cctcgcccgc
     9361 gacccctggg aaaccgccga ccgcgcccgc aaacttctcc aggacggctt ctctttcgcg
     9421 aatagtctcg gcatccacac cggcatcggc ttcgagcctt accagatccc cgacgagatc
     9481 tggcgtgccc tcccgccgga ggtcaagcca caggagctgc ccggcaggaa ttcagagggc
     9541 ccacgatttg acatcgaatc ggtcaccgcg cgcaagctcc tggaaacgcg cctcggccag
     9601 ttgctcgaag cctatcccga cgtcgaacac gtctggctct gggaagacga gcacatgaac
     9661 tgggagagcc ggcgtaccgg ccagcccttc tccgtcaccc cgttcctcca ggcacatgat
     9721 tttctccgcc gccacgcgcc gaagaagcgc ctggttgtcg gcggatgggg tggcgtcgcc
     9781 cgtcacttcg acgacttcca caagcgcttg cccatggacg tcatcttcac ctgcctcagc
     9841 gactctctcg gctgggaccc cgttcacgaa gtcttcggtc gcctcgaagg ccgtgagcgc
     9901 tggcccattc cgtggcttga ggacgacccg gccatgtggc ttccgcagtt ccacgtccac
     9961 cgcttcgaga tggacctgaa ccgtgccgct cagttcggct gccagggcat gatcggtatc
    10021 cattggcgtc accgcatcgt cgatccgacc gccgccttcc aggctcgctt tagttgggac
    10081 ccgaagctcg cgcccgcagc ctattaccgc gcgtacgccc gcacccaggc cgccggcagc
    10141 cgcgtggatc ggctagcgga cgtgctcacc gacgccgaca agaaccgcaa gctgctctcc
    10201 acgttcaccg gcgagttcaa ggacggccat gccgtcaccc gggaattctc cggcgactac
    10261 ggcgaagcgt tcgccttctg gaacgattac gagcccgacc tcgcggtcgc cgcctcgcag
    10321 aaggaagttg cctccgcctt gcggcagttg gcgggcgccg cggcatccga caccgaaaaa
    10381 gagcggctcg agtatcttac ccgccacgtc gagttccttg ttccctacgc cgaagcctgg
    10441 accctcgctc accgcctcca cggcgtgctg aagcaagccg gcgagagcaa gacggccggc
    10501 cgtgtggacg aagcccgctc caaagtacgg aatgagggag tccctctctg gatgaagctg
    10561 gcgcccgagg tccgcgccgc catgcttcac ttccagcgca tcgtggccaa ccgcaacgac
    10621 ctcggcacgc tcgcctccat gcagaacaag ttcgttcgcc tggcgctcta ccgcctgccg
    10681 ctctcgatcc aggattacct tggagagatg ccgccggaga tcgaatcgct tcgggagcag
    10741 atgcttcggc cggactccga agctcctgcg cgcctcttcc tgcccacgcg cccgacgctc
    10801 ctccgccctg gcgagtctct gcgcatcgtg atcctcgccc ccggcggccc gccacccgag
    10861 agcgtcgcgc tctacacgcg cgtccgcgga gcccgggagt ggttacccgt tccggcgaag
    10921 ctccttggcc ggcgcacctg ggaagctacg ctcggcccct tccgtgcgtc aggatcactg
    10981 atcgactact acgcccgcgc cggccgcctc accaccccct tcgacgcccc ccgcctcttt
    11041 tacaccgcca ccctccccta acccgggaca gtacactcat ttcctaattt cgatgctccg
    11101 ccgcggcgtc agcctctact tcaccgccgc ttggagttcc ctcgccaggt ctcctcgccg
    11161 ccccaccgcc accgcctcca gccttagcca ctccgccatc gtcctgagtt ccgacgccag
    11221 cgcccgcgcg acctcgcccc gattcacgcc ccgttccgca tacgcagccg gcacgaccag
    11281 cctgcgaccc gaacgatccg ccttcacgtc cacccgcgcc accagcccgt cgcccagcag
    11341 gaacggcagc acgtaatacc cccaccgacg cttctcttcc ggcacaaaga tctcgacccg
    11401 atactgaaac ccgaacagcc gctccgcgcg cgggcgatgc cacaccaccg gatcgaacgg
    11461 cgcaaccagc gccgctgcat cgacctgccc cggcgcacgc gcacccgcat ccagatacgc
    11521 cgtttccggc catccctcca cccgcacctc gcacaattcg cccgtctcca ccagttcctc
    11581 gatccgcggc ctcacctccc gtatcggcat gcgatagtaa tccgccaggt ccgccgccgt
    11641 cccgaccccg cacgaccgcg ccgccatccg caacagttcc cgctgcgcct cctcgcgcga
    11701 tacacgccgg tcaaggtgtt cgaccggcac aactcgctcc gccatgtcga acacccgcgc
    11761 gaagttcggt cgtcttcccg ccaccgccag taccccgcgc ccgaacaacg cttcgagcac
    11821 cgcccgcgga accgtcccga tccacgtacc ctccagacgc cgggaaacgc cctcgcgctc
    11881 cggcagatca tccgcgctca gcggcccacg ctctcgcacc tcgcctagca cccactccac
    11941 gtaatcagga ttccgctcca ggaacgtttc gaagccccac ggccgcacgc gatggttctc
    12001 catccggtga cgcagcagtg gccagctttc caccggcacg atcgacgctt catgcgccca
    12061 ctgctccgtg aacccacggc gcccgtatac cactccatcc agaagttttc ggtcatatgg
    12121 ccccaggcgc gaaaacggcg ccagatagtg cgccggagtg agtacattga cgtagtcgat
    12181 ctgcaccagc ccgagttgcc ggatcgtccg cgcaatatcg cgcgccccta cccgcccgtg
    12241 tggcggccgt gaacggtcga atccctgcgc cactaacgcg atccgccgcg cctcgctcaa
    12301 ggaaagttct tccccatcca tcagcccctc atactagccc attgaccctg caaccctccc
    12361 ggcgcacaat ttcaatagga cggaacatca agcgaaaagg ggggctgact ccttgttcac
    12421 ggtgacgttc tcccacccaa aacttcggga gcaggctttc agtggggacc tgtacggcac
    12481 ggcgctcgcc tacgttcgtg gtgacgtcga cgtagccggc gatgtcgtcg cggccgttcg
    12541 cttcttcgaa aaccaggctc gtcgcggctg gcggcaggct cgccacaacc tgctcggccg
    12601 aatgggccgc tggcgcctcg agccatggtg gcagagccgc cgccgtgcag ccgagaatat
    12661 ccgccaccac tacgaccgct cgaacgactt ctacctccag tttctcgacc gtggcatgac
    12721 gtattcgtgc gcctatttca aaaacccgga atgggacatc agccgcgctc aagaggcgaa
    12781 actcgacctc atcctccgga agctggacct ccggccgggt gaacggttcc tcgacatagg
    12841 ctgcggctgg gggtctctgg ttgaggaggc cgcgcgccgg ggcgccgcgg caaccggctg
    12901 cactctcagc cccgcccagc atgaattcgc cctcgaaagg ctccggacgg gttccgccgg
    12961 ccccaccgtc ctccgcaccg actaccgcga cctctccggc cagtgggaca agatcgcttc
    13021 catcggaatg ttcgagcacg tcggccgccg gcgtctcccc gcatatttcc gcaaagtgta
    13081 cgatctgctc tcccccactg gccttttcct gaaccacggc atcgttcgcc cgcaaaccgc
    13141 ccgggagggt gttgagtccc tcttcctccg ccgaaaagtc ttcccaggat cggagctccc
    13201 ccacctcgga gagctaatcc gcgcctccga ggaagccggc ttcgaagtcc tcgacgtcga
    13261 aaacctgcgt cctcattacg ccctcacttg ccgcgcttgg gtcgaacggc tgttgcggaa
    13321 cgaggaagcg tgcgtgcgct tcgcgggccg cgagacgcac cgtacctggg tgctctacct
    13381 cgccgcctgc gcgcaccagt tcgagaccgg ctgcatggac atctaccaga cgctgctcgc
    13441 ccgccgctcg agcccccacg gccgccgcct cacccgcgag tatatgtata acggcgtgag
    13501 cgcaagggcg gaagtttcag gtagtgaggg ggcgccgcgc tgtgcagatg taacccaggg
    13561 tgaaccggtt atcccgatcc aggaagtcaa gaagcggcag cgccagggct agtggagcca
    13621 ataagagcgc ggcgagagga aagacgatca ctggaataaa ctcgagtcca tcgaaaagcc
    13681 gccgtgacag caatcggaag agtccaccca ctggtttgat gataatctct gtaaatccag
    13741 cactctctag caggtactct actccgaacc gagtgaaacg atagtagtcg tgcggcgcct
    13801 ggttgatctc cgattcctgc tggacgacca gcaggagtcc tccgccgggc ttgagagtcc
    13861 ggacgagctc tcgcagaacc agccccggtt cacaaacatg ttccagtgtc agcaaattga
    13921 cgcacgcgtc aaagcgtttc tcgggaaacg gcagagtcgc cgggtcgcca atcacgtgag
    13981 gacggcgctg gctgcgcccg ttacgagcgg ttgagagttc taggctgcaa tagcgatgtc
    14041 tcttgaaatg actggtattg ccgatttcgc ctgagctcac gccgagcacc cgcgagtcgg
    14101 cagcggtgct ttcggtgaac tccgccacgg ttctctcaac cacttcttca aaatgaagga
    14161 gcgactgacc gatcatctca gccattcgca ggatcgggcc cttctgcgca gagcgtgcgc
    14221 gtttggacgc tttaagagtc gtatgcatgg ctcgtccctt cccctcgtcg tgcctctgcg
    14281 ccggcagcta ttcgatgagc gcgataacgg tccgtacgag tggaaggttg aggggtcgta
    14341 ggtgcacgtt cgagttggca tggaccttca gagcgcccgt gatcagttgc agcggcgcgt
    14401 cgtagccgcc tccgcctagc atcgaggtcc acgatccgcg aggctgatag gatacgcccc
    14461 aaaaatgaag gttcggtgac gcctgaaaca ccagttcggt cgatgccgag ctccacaacg
    14521 ccgtgttcgg gcattccggt tgcccgcaac tggtgtcgat gtatcctctc cctgtgtact
    14581 tgatgattga gttcgcttga tcctgccaca gaacgaccgg agcgtagagc ttcatctcgc
    14641 taggcaatgc ttcgtgctcc ctgttcaagc cgtgcaggtt gatagatact gacgagctgt
    14701 tgccggattg gaagcccgac attccgtgcc tcaggtctga agcaatggtc tggacaagtg
    14761 gcggtatttc gagcgtcgcg gtcccatccg gcccgccgtg gtagttcgga tcggtaaaga
    14821 cgaagatctc gccggcattc gctggcgtat cgtacccggt cgtcttgtct ttcagagtca
    14881 tgttgacgct gatatcaaac agcggggccg gcttgttggc ttgcggcttt gccccaacga
    14941 agaagtaact tcccggaccg aaggtgatgg ttgtgccggc ggactggttc gttaccccgc
    15001 cgaagaaggc gtaccgtccg aagccggtac caccgtcgcc aaactcgagg tttccttgca
    15061 ggcgaatggg gtcgcccgtg gcgtacggtt gcccctgtgc gttaatcgcc atcgcataat
    15121 agtttcccgg ttgcacaacc agagggttgt tgggatcggc gcttccggaa agcgttcccc
    15181 caagcacggg acggtccgct aggccccttg gaggtggtgg ttggcctttt ccgcgcaccg
    15241 ggtccgtgaa atagcgcgtg tcgggttttc cgctgtcggg ctgtgcggtc caagtcgcgc
    15301 ccccggacaa cgtgtaactt ccggactctc gaatgtaggt gaaaggggct ataaccgcgc
    15361 cgcccccggt gttctcgccg gcaaacctgg cgtctacgtc agccccgtgc ttggtagagg
    15421 ccatcatgat tccgccttcc gcttgcaggg cgtgcaggcc ttggttgtcg ttcgcttgaa
    15481 tcaacgcatt cactccgcaa actgaggagt tcgcatttcc cataggcagg cagtcgttct
    15541 cccggttcag caaaatcagg gagcccggaa caatggagtc gaccacaccg gccgtggctc
    15601 gaaccgatga cgttccgacg aggttcccca tcatcgcgga gaacaactgc gggatccgct
    15661 cggccgccgt cgccctgacc cagtagttga cctttacccc ggacacgtca ggaacagccg
    15721 gacccacacc cgacgtcatc accacctgct gccgtccatc cctgccgccg gtacggaacc
    15781 cgttctctga ggcgtagaga caagctacct cgatgtcact tgagggtgga gtcccaatcg
    15841 atgctggaca gggagtggcc tcctgacagg tcatgccggc gccgcacgtc catgggccct
    15901 cctctcctac cccttcgagg acgctcgtgg ctgcggcgag aacggacgca tccacggccg
    15961 cctgggccga tttctttaca aaatacatcc agcccaaatc aagtgccaga cccaaaactc
    16021 cggacatggt tagaagcgac agagcgacca tcggcaacac ttggcccttt ggccttctac
    16081 tgtacctttt tgaaaccata tttcacctca ctactaatcg ccacggaccc aaagtggtca
    16141 gtaaatcggg agtcccttcc cggctccgat ggtcttccca aacagaagga cgctgtaagc
    16201 cccagggatg gagaccgagg ccccggtgct ctcgtttctt aaaccgatct cgtcatagat
    16261 cacgtcatgg agaaccagga actgtccgac aggacacgct tccgttgggc caggcggcga
    16321 caaggagatt tcccctgaca cggtgccggc tttagacgac gtgaaagcgt cagtggacca
    16381 aacggctgaa ttcaccgccg atctcggtgt gccggtgggg tgtctggtcc caccctcgac
    16441 gcaccaatag gttgccgtgg cagtggccgt gactaggtac gagtactgga gccccggaga
    16501 cagtcccgtc tcccgccagg aaacctgcag tttgccgcct gagtccgcac tcgcctttgc
    16561 ttgtgtgaat tctgcagcga ctgccagtcc ggacatggcg agaagcgaca aagtgaccag
    16621 cgggaaaacc tggcctcgcc gcctttggaa ggacctgcct gaaaacatat atcacctcat
    16681 taccaagaag atattagtac tatacgtact aaaactatca tatccgatat aatacgtcgt
    16741 ttcggaagaa tgtcaagagg ttttcttccc ccaattgagt ttttttcgtg tttcactcag
    16801 gatttttgtc ggactataat tgaactgcca actccggcga cacggtgctg ctcatggatg
    16861 atgctacccg ccgccacttc ctcgaagcca cgctgctctc tgcgggggcc ctcgactcat
    16921 gtagccccag cgcatccgtg agcgctcccc ggacgatgga gcgcagggac tttgagcccg
    16981 cctatctccg cctcgaacgg accggtgatc tcgccaggcg tgcccgggag ctttattcca
    17041 tctatcgcgc ttgccgcctt tgtccgcgcg cgtgcggcgt caaccggctg aaaggcgaga
    17101 aaggtgtctg ctcctccacc tcccgcgcca aggtttactc cgcccactcc cacttcggtg
    17161 aagaacgccc gttggtcggg cgcggcggct ccggtacaat cttcttcagc aactgcaatc
    17221 tcctttgcgt tttttgccag aactggaaga tcaaccaccg cggcgaaggc tcctacgtga
    17281 gcgacgacca gatcggtcgc ctcatgatcg acctccagga tatcggctgc cacaacataa
    17341 acctcgtcac ccccacccac atcgtcccga atatcgtcca aggactgcgc tccgccgtcg
    17401 ccaggggtct gcgcatccct atcgtctaca actgcggcgg ctacgagccg gtcgaaatcc
    17461 tcaagttgct cgacggcatt gtggacatct acctgcccga ctacaaatac accgacggca
    17521 ggatgtcgga gaagtattcg tcgggtgcgc gcgactaccc cgaggtggcc gccgccgcta
    17581 tcgaggagat gcaccgccag gtcgaagagc tcgtcgtgga tgagaacggc gtcgcccttc
    17641 gcgggctgat cattcgccat ctcgtgctgc cacagaacat cgcgggcacg gacaagttcg
    17701 tccgattcgt cgcgaacgag ttgacgccct caacatacgt gaacatcatg gcgcagtacc
    17761 ggccggagca cctggcgcac aagtatcccg agctgtcgcg ccgcctcacc cgcgccgagt
    17821 accagcaagc ggtcaactgg gcccgcgccg ccggcctcac acacataacg ggggcctgac
    17881 tccaatctcg ctgcctttgc gaaacttgac aacgattccc gcctacgcta tcgtatttcg
    17941 atatcgaagt tcgatgacag aaactcaagt taaagtgacc cgcgatctct gcatggggct
    18001 gatccggctt catgttctcc accatgcagc cgaagagccc atattcggtc tcggcatcat
    18061 ggaagagctg gctcgtcatg gctaccgctt gagcgccggc actctgtacc ccatcctcca
    18121 cggcctcgaa cggcgaggct acctgcgctc gcgcgtcgaa cgccgcggac gcgtgaaccg
    18181 gagggtttac cgcgcgacgc cccttgggcg gcgggcgctc tccatggcga aaaggaaagt
    18241 gcaggagctc ttcggcgagt tgtttgagga gacgacccat gacctgctgt cttgatcaaa
    18301 accgtcagca aaaccgggga ctggctccaa tttcgccctg tttgcgaaat tggtggctgc
    18361 ccccgcgttt tgcatggcac ccggttctac ccctcacctt ctacctgacg cttctccttg
    18421 ccctcggcgg ctctctggcc tgtgccgagc agccgctcac catcgggcag gtcgtggcta
    18481 ctgccggcga gcagtatccg tccatccgtg tctccgagga gcggatcagc tccgccgctg
    18541 ctgcggtaag cctggcgcgg acctcgtacc tgccccgcct cgattttctc gcccaggcga
    18601 atcgcgcgac acgcaacaat gtcttcggga tgttacttcc tcaatccatc attccgtcga
    18661 tctcgggacc gccctcaatg accaacagcc tcacgagcgt ttgggggagc accgtaggtc
    18721 tgctggcgac ctgggagccg attgacttcg gcgtgcgcgc cgcgaaggtt cgtaccgccg
    18781 atgccggtcg cctccgcgcc gaggccgcac gtggccggac ccgtttcgag gtaagcgcgc
    18841 tggctgccga cgcatttctc accgtcctcg cggctcagca gacgctcgtc tccgcacaag
    18901 ccggagtcga aaggtccgga accgtggtga aggtcgtaaa gtccctggtg gactcggaac
    18961 tgcgtcccgg tgcggacctg agccgcgctc aagcggaatt ggctctagcg gagacacagg
    19021 tcatccaatc gcgcagcgcg atccgggtgg cgaaagcgtc cttggctcaa ttcacaggcg
    19081 gagaccccgg cgccatgacg ctcattgccg gaaaattgct agacctgcct gcctcgaagc
    19141 ctctacccgg cgaaagcgcc gcacagcacc ccgctgcggc cgaacaggcg gccgcctccg
    19201 cggaagcagc cgcgaaactc agagagttgg atcgatccta ctacccgaaa ttcctgctcc
    19261 agggtgcgag ttacgcccgc ggcaccggcg ccattccgga cggaaccgcc ctgggcgggg
    19321 tgaacggact gggcccgaac attcacaatt ggggagtcgg cttcaccgct acattcccga
    19381 tcctggacct gcctgcgatc cggtccaggc ggcaggagga gcagcatttg ctccgcgccg
    19441 agcaggcccg cgaggagcag gtgagacggg agctcgacgc cggccttcag cggtcgaacg
    19501 ccgcactgga gggagcctgg gaggtcgccg ccaagacacc ggaacaggta gctgccgccc
    19561 atgcgacgtt ggagcaggcg tcggcccgct accgcgccgg cctcggaacc atcgtggacg
    19621 tcgcggaggc gcagcgcctg ttgacgcagg ccgaaaccga cgactctctc gcgcgcctgc
    19681 aagtctggcg ggcgatgctt gctggggcag tggcccaggg tgatctcggg ccgttcctgc
    19741 aaacggccgc ccaataaacg gagggaccta tgtggttggt gaacaccgct cttcggcgtc
    19801 ccatcaccgt gctcgttgcg atcctcgctg tcgtcctgtc ttcctggctg gccgtcaggc
    19861 gaatgcgtgc ggacatcttc cccagcctcg gagcaccgac catttacgtt gcgcaacctt
    19921 acggcggcat ggatccctcg cagatggagg gcttcctcac gtattactac gagtaccact
    19981 tcctctatat caccggaatc gagcacgtgg aatcgaagag catccagggc gtcgccttga
    20041 tgaaactcca gttccacccc gacaccaata tgaaccaggc gctgtcggaa accgtagccc
    20101 aggtgaaccg ggcgcgcgcc ttcatgccgc cgggcgcggt ccctccgttc attgtgcgtt
    20161 tcgacgccgg cagcgttccc gtcggacaac tcgtcttctc tagccccatc cgcagtcccg
    20221 gcgagatgca ggacatcgcc atcaaccgcg tcagaccgct ctttgcgacc ttgcctggcg
    20281 tgtccgcgcc gccgcccttc ggcggcaatc agcgtactat cgtcgtacgc ctcgaccccg
    20341 accggctccg gggctacggc gtttcgccgg aagaagcgat ccaggccgtg agcaaagcca
    20401 gcaccgtgct gccctccgga agtgtccgca tcggggatca gacgatcatc gcatcgacta
    20461 acgtcgtcat cggcggcgac ctgaacgagt tgacgggcgc cccggtgcgc acgggcaacg
    20521 gcccggcggt ctatatccgc gacatcggcg tggtggagaa cggcgccgac atcatcaccg
    20581 gttacgcgca cgtcaacggg cggcgcacgg tctacatccc ggtcaccaaa cgctccgacg
    20641 cctccacgct tgccgtgatc cgggaggtga aagaggccct tccggcaatg aagaacgcgg
    20701 tgccggaaga tgtggacgtc cggctcgagt tcgaccagtc gggctatgtg gcccgcgctc
    20761 ttcgcggact ggtcaacgaa gggctgctcg gtgcgcttct caccggcttg atggtgctcg
    20821 tctttcttcg cgactggcgt agcgctctca tcgtcgtcgc aacgattccc gcggcgctcc
    20881 ttgccgcggt cgtgtggctg tgggcggcgg gccagaccat caacattatg accttggccg
    20941 gtctggctct cgccgtcggc gtgctggttg acgaagcgac ggtagaaatc gaaaacattc
    21001 acacgcatat ggctaccgga gcatcgcgtg cttgggcggt tctcgacgcc tgccggaaga
    21061 ccgccctgcc ccggctgctc tcgatgctgt gcatcctttc ggttttcgtt ccgtcgttct
    21121 tcatggcagg cgtgggccgg cagttgttca tcccgctctc gctcgctgtc gcgtttgcga
    21181 tggtttcgtc gtacttcctg tccagcaccc tcgtgccgat cttgtcgacc tggctcatgc
    21241 gaaggagcca ggggagcgat gaagaccggg gcttcttcgg cgcgctgcgc gccctctacg
    21301 cccgctacct cggattcgtg attcgattcc gttggccgct ggtggcggca tactcgctga
    21361 gcgttgccct tctgatctgg ttgatgttcc cgcggctcgg aacggagatc tttcctgtcg
    21421 tcgacaccgg ccagttccag ttgaggctgc gcgcgcccac tgggacgcga atcgagcgca
    21481 ccgagatcat gaccctgaag gcgctcgatc tcatcacgcg cgaagttgga ccagacaacg
    21541 tccgcatctc gacaagcttc atcggggttc agccggcaag ctaccccatc aacacgattt
    21601 atctgtggac gagcggtccg cacgagtccg tgcttctcgt cgcgctgaag cctgccgcca
    21661 agctacgcgg tgacgcgctg agagaacgtc ttcgcgcgag gttcgctcag gaactgccga
    21721 acgtgaaagt gtcgttcgag gcaggcgaca tcgtcagtca ggtgatgagc ttcggatcgc
    21781 ctaccgcggt tgaggtggcg gttcaggggc caagcctacc cgccaaccgg gcgtacgccg
    21841 aaaaggtcta cgcggagctc gcgaagctcc gttccctgcg cgatgtgcag tactcccagg
    21901 ctctggacta cccgactctg caggtgaacg tgaatcgcga ccgtgcgggt caatacggcc
    21961 tcaccgcggc cagcgtcgcg aagtcgttgg tcgcggccac ctcatcgagc cgcttcatcg
    22021 agccgaacta ctggcgtgac ccgaacagtg gaaacgcctt ccagattcaa gtcgagatcc
    22081 cccagcaccg catggcctct ctcgacgatg tccggtccat tcccctggcc aggaacggag
    22141 gaacccctgt cgagttggcg gacgtcgcgg agctgaagat ggcgaactcg atggggctgg
    22201 tcgagcgata caacatgcag cgcgtcgtca gcatcaccgc caacatccac ggcaggacgc
    22261 ttggcgaaat cgcgggagag ctgcggcagg cgctgaaacg ggtcggcaac ccgccgaggg
    22321 gtattgccgt cggagtcagg gggcagatcc cacccctcga ggagactctc ggcggccttc
    22381 gcatcggcct gctgctctcg atcggagtca tctttctgtt gctggccgcc aacttccagt
    22441 cgttccgcct cgccctcgcg gtgatcgccg tgattccggc ggttctgtgc ggcgtgctgc
    22501 ttattctgct ggtcacgggg accacgttga acgttcagtc gttcatgggc gccattatgg
    22561 ccatcggtat ctctgtggcg aactcgattt tgttgctcag cttcgcggaa atgagtcggg
    22621 cggacggagc cgagcccggc agggcggcgg tcgaggccgg ccgcggccgg ctccgcgcca
    22681 tcctgatgac ggcgaccgct atgatcgccg gcatgattcc catcgccctc gggctgggcg
    22741 aaggttcgga gcagacggcg ccgctcggac gggccgtgat cggcggcctg gccgcggcga
    22801 ccttcgcgac tcttacgatt cttcccgcct tctatgcggt ccttcagcgg cgcgcaacgg
    22861 cccattcggc gtcgcttcat cccatggatc ccaggagcag attctatgaa gtccgttagt
    22921 gtgcttgttt tcgcgcttgc atcgctctgc ttcggccagc cggcggacct cgtcgcggtg
    22981 gcctcacgcc cgttggagcg aacggtcaaa ctgcccggcg agttcgcccc ttacgaacag
    23041 gtcgacttgc atgccaaggt ctccggctac gtagacaagg tcctcgtgga ccggggaagt
    23101 gtggtgaaag aaggcgacct cctcgtgacc atgagcgcgc ccgaggtgaa agcgcaggtc
    23161 gcggaggcgg acgccaaagt aaaagctatc gaagcgcaga tggcggaagc gcgcgccaga
    23221 attctcgccg cgcaaagcac gtacgaccgg ttgaagcagg cctcttccac gcctggcgcc
    23281 atcgctggaa acgagcttgt tatcgccagg gagacggtcg aggcggccaa ggccgctctc
    23341 atggcccagg agggtgcccg ggacgccgcg cgagcttcgg cggacaccct gcgtgagctg
    23401 caaaagtacc tcgatatccg cgcgccgttc tccggcgtca tcacggaaag gcttgtgcat
    23461 ccgggcgccc tcgcagccgc gggaaccggc gccgccgctc cactgctccg gctggaaagg
    23521 ctctcctcgt tgaggctaac cgtcgcggtt cccgagtctg cggtcggcgg ttcgctgccc
    23581 cggggacaag tggagttcca ggtaccggct ttccccggcc gttcgttccg tggaacaatt
    23641 gcgcgcgccg ctcgtaccct cgacccgaac acccgcacca tgcccgtgga gatcgatgtc
    23701 cagaactcaa acggactgct cgcgcccggt atgtatcccg aagtgttatg gccggcccgg
    23761 ggcctcggcg cgtcgctgct cgtccctccc gcgagcatcg tcaccacgac cgagcgcacc
    23821 ttcgtcatcc gctccactca ggggaaagcc gagtgggtaa ccatccgacg cggcgctgcc
    23881 gccggcgacc tcgtcgaggt ctacggtccg ctaaagcctg gcgaccggat tctgaagcgc
    23941 gccaccgacg aaatccgcga aggcacgaag ctcccctaga gcccctactg ccccaccggc
    24001 ggcttcaccg gcgacgggcg ccgcccttcg cgatccgcct tccgaagcag cgcatccttg
    24061 atacgcttgt ccacagtgtc ggcctcgcga aggcgattcc ccgcgcgaag gaccttcgcg
    24121 taaatgcgaa gcagcgggtc gagcatttcg tccggcgcct tcgccagatc gccgatcccg
    24181 atggccctat ggtacaagtc ctcggcttcc ccgagtttcg cctccatcaa caatacccgc
    24241 ccgacttggt tcagactggc gatgtgcgcg gtagtcctga tagtgagagc ggcggacgcc
    24301 gccttttctg cttcttcgta gcgctgttgg aacgcataga actccgccat tttgtccagc
    24361 gtgagcgcaa ccatcgggtg ttcgagcccg gcgcttgact cccagagggc gagcaggcgc
    24421 ttgtagaccg gctcggcctc ggaaaatttc ttttgcccga agtagacgta cgccagtgag
    24481 tccagggtcg acagcagttc tgaggaatcg ggtcccaagg ccgcttcacg cacccgtagt
    24541 gcgcggaggt acagcggttc ggctttctcg taatcggcgt tggtctggtg gatgcccccg
    24601 agtgcatcga gcaccggcag aagttgcggg ctgtctgggg caaccgcggc ttcccatgcg
    24661 gtcagcgcct gtttcagcgt ctcctcagcg aacttcgcgt cgcccagcgc cacttgaaga
    24721 atcgccacac cggtgatgtc tctcgcgagg tcctccgctt tgccgtccgt gcggctttgt
    24781 cggatccgca ccgcactctg caacgtggtg agcgcttctt ggcgtctgcc gttcgagcgt
    24841 tgaaccgccg caagttcgga caacaacgca gcaacctcga ggctcgcatc gccgtgcaac
    24901 cgcacggcta ccacaaccgc ccgcgagagg tactgttctg cgtccgcgcc gtgcccctgc
    24961 aagcgcgagc cggtcgcgca accacggatc gcggcgagca ctcccgcatc ctcccccggc
    25021 gccgtctcca actgcgccag cgccgatcgg aaccttgccg tcccctcggc atagtcgcgc
    25081 gcggcgatcg ctttgtttcc cttctcgatc tctgccttcc aatcgacccc gggcacttca
    25141 acgggcgcct gcaaggtcag tagggcgagt atcaccatca cggttggcgc catgtctgat
    25201 ctccttctgt gcgtctggca ggtgcagtta ccgggagcgc aagctcagcg ctccttcttc
    25261 agcaccctct tcagaccgtg ctgctagttt tctagcacct tactagacac cagagccgca
    25321 caaagagttc ccgctggatg cgagagtaag cacgcattcc ggcgtcggaa aggaaaccaa
    25381 ttccgatccg gcggtgtcaa atcggcatgc ggcgcgcacg ctgattgccg cggaggttgc
    25441 gatgcgcaag gatcttctct acgccgtccg taacatggcc cggaatcctg gagtttccgt
    25501 ggttgccgtg ttgacgctgg tgctcggtat tggagcgaac actgccatgt tcagcgtcat
    25561 tcatgccgtt ctccttcggc ccctccccct ccgcgagccc gagcgtctgg tgaccatctc
    25621 ggccgggatc ccgagcatga atatcaggaa tgcgtctgtg gaatacaaca ccttcaccga
    25681 gtggtggcgt gcgcgtacac gctcgtttga agcgatgtcg gcttacacgc cggagacggc
    25741 aaacctcact tcaggcgatc agccgcaacg cgtccggatg ctgcgcgtga gcgccaatca
    25801 cttttccgtc gtcgggacga ggccggcggc tggccgtgac tttctggacg aggaagaccg
    25861 gccaggcgct ccgcgcgttt ccattatcag ccacgggctg tgggagcgcc gcttcggcgg
    25921 ggaccgcgcc ctcctcggtc gcgcgatcgt gctggacggc aaaaactaca ccgtggtcgg
    25981 gatcctgccg tccggcttcg acctcaaccc ggaggacgtg tttactccga ttgcgcatag
    26041 cggggcgcga gtgcccgaca tgccgacggt cggcgcctac gcgcgcctga agcgcggcgt
    26101 cgcggtcgag accgctcagg ctgagatcga cggtctgtgc cgcggctggg tcgcgcaata
    26161 tcgctatccc aaagactggg gcgctcgtgt gtggccgatc cgcgaattca tggtgcgcga
    26221 cgtgcgggcg agcgtcctgg tgcttgcggt cgcggttgcc ctggtcctgc tgatcgcgtg
    26281 cgcgaatgtc tccaacctgc tgctcgcgcg cgccggcgac cggcaacgcg agatcgcgat
    26341 ccgcacagcg ctcggcgccg gccgcaagcg cattgtccgg caactgttga cagagagcgc
    26401 gctgctcgga acggtcgccg ccggtctcgg gctggcgctc gcctgggccg cggtccgcgc
    26461 gctggccgcg gtcgagctgc cctttccgtt cctgcgcaaa gtctccatcg atgcgcccgt
    26521 actcttgttt accaccgccg cgatgctgct aacgaccatt ctgttcggct tggcgccggc
    26581 agttgccggc gcgcaaaccg gcctggtcga aaatctgaag gaggggggcc gtggggccgg
    26641 cgaaagcatc cgccgaagcc gctttcgctc ggcgctggtg gtggccgagg ttgcgctcgc
    26701 gctcctgctg gtcatcggcg cgacgttaac cgtccgcagc ttggcccggc tgatggcggt
    26761 cgatcctggc ttcaatccgg aaggcgttct tacggcccag ctcacgctgc ctcagtcgag
    26821 ctacgccgaa ccggttcgcc gcgtgaactt cttcaagacg gtgctggaac ggctcgcggc
    26881 gacgccgggc gtaaaggcgg cgggcatggt ctcccacctg ccgttcggcg ggtccaagtc
    26941 cggtctggat gtcgtgcccg aaggcgcgcc gccggtccgg ccgggcgagc agaagatcgc
    27001 cttcgcgcgt gcgaccgatc ccgaatactt ccgcgtcatg caggtgcgcc ttgtccgcgg
    27061 acggtttttc accggacgcg atcccgcggg ccctccagtc gcggtcatca acgaaacgat
    27121 ggcgcggcgc tgctggcccg gccaggaccc ggtcggcaag cgtttcggcc tcggccggac
    27181 cgacttccgg ctcacggtag tcggcgtcag cgctgacatg cggaacacgt cgctggccga
    27241 cgaaccggac gccgagtatt tcgcccccta cgcccagagt cccggcgcct cgatggcgct
    27301 ggtggtccgg accgagctcg atcccatgag cgttgcctct gcgatccagg cggttgtgcg
    27361 cgaactcgac aaagatctgc cggtatccga ggtggcgacg atgtcggcga agatctccca
    27421 gtcgacgagc acacgccggt tttcggtcgc cctgctaggc atcttcgctc tgatcgggct
    27481 gatgcttggc gcggtcggca tttacggggt ggtctcgtac tcggtctcgc gccgcacgcg
    27541 cgagatcggc ttgagaatgg cactcggcgc agccggcggg cgcatttcgg cgatggtggt
    27601 cggccgggca gtactgctcg cggcctgtgg tgtggcgatc gggattgccg gcagcctcgc
    27661 gctgacacgc ctgctcggca gcatgctctt cggcgtgagc gctactgatc cggttgtgtt
    27721 cgcggcggcc tctgtgttcc tggtcgcgtt atccgcgctc gccgcttaca tcccggcccg
    27781 ccgtgccagc cgcgtcgacc cgttggtcgc tctccgccac gagtagttct gtgcgagcga
    27841 aacccgggac tgactccaaa tttcgctgct gttgctccta tgcggcggcg gagcaagaac
    27901 ggggactgac tctccatttt gccgctgttg cgaaatgcgt gtctgtcccc gcttttgcgg
    27961 cgaccggaga tggagcagct tgtgtaccat ctctgggagg aatccggccg gcccaccggc
    28021 aaggccgagg agcactggta ccgcgcggag caactcattc acggcgcggc ctgatcacat
    28081 tttaggctcc tgcgcaatca ctggatcagg ctggcaactt ttgacgtcaa gttgacgaaa
    28141 cttatcccaa tgccccaata ctggcggtcc tgctctacgt ggaggttcgc tccgtacgtg
    28201 aactgcaaga tattgttgaa gagcgagaac tggacgccaa ggcctacttg cacgtcgctg
    28261 cccttggtcg cgggtgcgaa cttccctgtt gagaggtcga acgctgggtc tttccagttc
    28321 atgaagctga cattcatccc cgcaccaggc cgcatgaagc ggagaaagtc gttcccccgg
    28381 gacaagtagg tgcctccgta ggtcactcca ggagacggcc cgaagttgat tgcatcgact
    28441 ccgttctttt tgtcttcctc gctgacgccc agccgcttga gaaacagtag gctgtccgtc
    28501 accttcaccc ggaaaccgaa cggttgcgga ctgactgtgt aaaccacaga ttcgagggtc
    28561 accagattgg tgatgcgaag ctccaggtat tcgtagtcgc caaatccgat cgagcgcagg
    28621 ttgatatcgg tgtccggcat gatgtcaccg ggattgagga aatccttgtc atggacgacc
    28681 acggggttct gctcgtttcc cttgcttacg tagtactgga tgggaacgag aggcagactc
    28741 gacttctctt tgaacagttc accgctgatg cggatgatca cccggggccg tccgccttcg
    28801 tccttgggtt gcatctttgc ctcggtgaag tcgatcacca gcttgccgcg ctcatctact
    28861 tcggagtcgc caggattcag ggtgagatcc tgtccgcgag gcggcctttc tttgaccgtg
    28921 acgaaatcct tgggatgggt ggactgggca ttcgctacgt tacagagtag aatcgtcaga
    28981 atcccgaggt agattgaatg tgttttgcga agatggctag tcatgggggc tccttttctt
    29041 cagggcagat ttcgcaccat gacgccgata tcgacgggcg gaggcgaaac cctccggtcg
    29101 aagaccgcgt tatttacaac agttacgcac tcgattgtcc gctggccgct ccagtgcggc
    29161 agatccggat cgccggcgtc cgatacgtcc acgtggcgaa tgttgaagct tttgccagtg
    29221 gcggacctgc atgcctccgc ttggtccaca atgtcaaaga cccggctgag ctggtcctgc
    29281 cccgatttaa agaagatcag aaacttgttc atcggcttcc ctcccctttg gggaacttgc
    29341 aggaagagca aattccctcg gatagtcgct agtgacggat gcgggtaaaa cgtttcaacg
    29401 agccagacaa aactcgtcat gaattttttt cacccagagg tacccgttct ctgaaccgat
    29461 ccaaacgaga gagagaaggc cgctctcatg aaaaagagag caccggcaag gaatctgata
    29521 ttctgagtgc accgcgatga accacgtaac ccggcgccgc tggctgcaga ccagcatgct
    29581 tctagccggt gcgccgtgcg cctgcctcgc caaagcatcc aacgattgct gcactgtgcc
    29641 ggaagcaccc ggcgatgccg tccgcatcca gaacgggctc ctcaccatcg atctaaccct
    29701 gacccccggc ctgaagcaca tcggctctgc cgtcaaggtc gtcgacccgg ctcgaaagct
    29761 gcgcgtcatc atcgcgcggc ccggcaagca ccagttcgtc gctctcgatc agaagtgtac
    29821 ccacggcggc ggcgcactca cctacgtgca cagacacaag cacctttact gcacctgctg
    29881 gggccactcg aagtttgcgt tggacggtag cgtactccgc tggcccaaca agctgacgcc
    29941 ccgcccactc cgcgcccacc cagtggagcg caagggtaac ctgctcgaaa tccgcgtgga
    30001 agggctcgca tgatccactt gctcctggcc gtcgcctgcg gcgtcatggc attcgcccag
    30061 aagccggagt tcgtcgaaat ccccgcgggt tcgtttatta tgggatgcga tcccgcttgg
    30121 aagtgcgccc aaagcctccc caagaagcgc gtcgacttcg atcgcccctt tctaatgtcg
    30181 aaaaccgaag tcacagtggg ccagttccgc gcgttcgtca aagctaccag ttaccggact
    30241 gatgccgaga aggcgggcga agcccgtaac tggcaatccc ccggtttccc acttagtaac
    30301 aagcagcctg tcgtcttctt gagcctcaat gacgccaaag cttactgcga attcattgga
    30361 gcacgcgtgc cgttcgaagc cgagtgggaa tatgccgcac gcgccgggtc cactacgcac
    30421 cactactggg gcgaggagat tgacggccgg tatctctggt atttcaacaa caccgatgag
    30481 cggccaatgc cggtcggccg caaactcccc aatgcctggg gactctatga tgtcgaaggc
    30541 aatgcgatgg agtgggtgcg cgatggcgga ccgcactcca gcatcacgcg ccagggtgcg
    30601 ggctctgtcc gcggcgcatc ctacgcttcg tgccctgaac cgtatccgcc gatggacggc
    30661 ctgcggcagc gtgccatcgg tcttggcccc acgttcccgg gtttcgagaa tagtaacttc
    30721 cgtcccgacg agcgccgctt cgactacggc gttcgctgcg cgaaatgacg cgcagaaacg
    30781 gtggctggct acgctgaccc aagcgtcgtg tgcgcgtggc agcgtagcct gtcaccgttt
    30841 cctattgaga cggctttcca ccgagatagc cgagactgcg gaggcgctcg atcgactggc
    30901 gatcgagcgt cgtctctccg cctggaccca gtagtttcct gatctggtcc tgggccttca
    30961 gccactggtc gatctcagcc ctcaacttct gggcgacgcc ggaattccgc gatgccacat
    31021 ttgttgtttc cttccggtcg gtccggcggt cgtacaactc cacttcgggc aatcctgcga
    31081 gccgcgcccg atcccggtac aacagcttcc acggcccgtt ccagatggcg taagtgtctg
    31141 tgcagttctc gggaacaagc ccgccgggtt tggcgaacgg aagagcgaag cgtgacgaca
    31201 tcaccggacc ctttcgctgg aacggttggc ccttagccag cggcgcgaga ctctgtccct
    31261 gcatcactcc ctgtggtgcc acgccggcaa gatcgaggat cgtcggatac acgtccagca
    31321 gttgaaccgt gtcggaaacg cggcggggag ccggtaacaa ttttggattc cacatcagga
    31381 aaacggcgtg cacaagctcc tggtagaggg agtggccgtg ccctgtcaga ccatgctccc
    31441 agaactcctc gccgtggtcg gagaggacca ccaccagcgt gttattcagg atgccaagtt
    31501 ctttcagctt gccggtgagg acttcaaggc tgcggtcggc gtgcgcgatc tcgccgtcgt
    31561 agcggtcgat ggcgcgcctg aggaacttgt ccggatcgat ccccttcgcc ttgcacttgg
    31621 cgcgcgtgac ggccatgccg ccgccatact gccgcttgtc ccatagattc tggtagtcgc
    31681 ggttgaactg ctccgtctcg gcgggattgg cgaatctctc ctcgaactcc ttggggggcc
    31741 gatacggggc gtggggatcg gtggtgtgga cgtacatgaa gaagggttcg tcccggtgct
    31801 gttcgaacca cggcattgcc accttgttga ctgccgctga gtccgtgccc cggtcggctt
    31861 cgtccgtacg caaccggtgt acaaccgggt attccatcat gtagtcgtag ccgcgattca
    31921 gcccggatgc acggcccgcg taaggatttg tgacgatgcc cacggtcgaa tatccggccg
    31981 cacgcagcgc ttcggccagc gtgaccgcac gctgtggaat cgcgtccgtg tcctggcgca
    32041 tcccgagtgt gtaactgtgg atcgacgtca gcaacgaggg caccgagccc ttcgtccatg
    32101 gcgcttgcgc ctgggcgtcg tcgaaaacga cgcccgccgc ccccaactgt ttcatgaacg
    32161 gcgtggtgtc gcgggaatac ccatacaaac tcgtgtggtc ggccctcatg ctgcaaacga
    32221 ggtagaccag cacattcggc cgggccttcg cggcagcggt tgtcagcaag ggattcgccc
    32281 acaacccgat ggcgccctgg ctttccgagg aggtttcgaa gatgatcttc gtcgtcctcc
    32341 cggcgtactg cgagagatcg acctcggcat ccgcccagcg ctcgggatcc tccagggtct
    32401 ttgagaacac tggactttga gacgggcccg cgagaacgcg gaaggtcacg ggcttcttct
    32461 cggcaacccc gatcccgaac ttcagccggc ccttttcggg tatcggcgcc tcgtacgtaa
    32521 ttgcggacgg cgagtgcgca tagatcgtgt tgcggtactc ctcgcctttc ccgaggacct
    32581 ggcgcccggc cgctttcggg aaactggtcc ggcgcggcac gagttcgatg aagtcaatcg
    32641 ccgccaggtg aaagggactg tcggtgggga tgacggccag tggccggctt ccggtgggcg
    32701 cctggatatc aacggcaaac cgatacacct ggtactgctt gggcggtgcg agcttttgcc
    32761 gcatgtcgag aggcccgatc ctgatcctga cttcagagcc cccctcagcc atcatccgga
    32821 tctgtacgct ttcgaacagg ctccagtcga tcgcgaactc cttgggcgag acgatgactg
    32881 gagactcgcc gtcgcccttc aggatcagat cgccgtcgcg caggcccatc ctcccgcgga
    32941 ccagttccca ggtggcgtcc ttctcggtaa agaaatcctt ccagacgacc ggatctgtca
    33001 ccctcgcggc ggtctgcccg cttgctgcgg acacggtagc agcggtttcg aggttgtcgt
    33061 caaaacgata cggctggtag ggtgcttcct gcttgtggga gccctgatcc cggcgcgcgc
    33121 aaccagccgc caacaacagg atcggcagga gaacggcaag atggcggaca catctcatga
    33181 ggggccgaaa ttgcaagtat acccacgcca agaaacccgg gacagtacac tcatttccga
    33241 gttttcatta gtgggggaag gccgggtccc cttacaactt tcgggacccg gcttttcagg
    33301 cgagaaacgg ccgcgggcta gaattcaacc cgaagcgaga attggatgat gcgcggatcg
    33361 ttgagctgct ggctgaggtc ctgcaggccc gttgtcccat ccggcggcct gtccaccggg
    33421 tggttaagcg cgttgaagaa gtccgccgtg atcttcgtct tgatcctctc ggtgaccaag
    33481 atcgtcttga acaaagaaat gtcggtattc caggagccag gcccaaagaa gaagttcctg
    33541 gcgttccagt tcagcatatc gccgatgccc gtggcgcgaa ccgtgccgtc agcgagctcc
    33601 atcggaatcc ggttgtcgaa gccgccgccc actggccgaa gcactcgctg agagcggtcg
    33661 gccggaatca acgccgcaag cttggacgcg tcgacgttgc tggccaggct cgtgtcgaaa
    33721 tcccccttga accagaggcg tcgtggacgg ccggcgtacg tcagcaggag ccgttcatcg
    33781 gcgctaaggg tcggatctcc aaacagatag cggccggagc cgacacctgt ccagaaaccg
    33841 cttctccagt cgccgatgaa ggcgatctgc cagccgccga tcacatggtt gagagcgccg
    33901 gaggccccgt ttccgaactt ttcgcccttg ccgaacggca agtcatagat gccgttccac
    33961 cgaattcggt gtgcgggaac ggaatcggag ttggcgtagc cgaggcgcag ccgctggtct
    34021 tcggagaggg ccggcgcgcc cataatcacg tgattctccg gcgccgcgaa cgccgtgccg
    34081 ctaccgctgt tattgatgcc gctcgagccg aagttgaagc ccccggtgtc ggtcgtggtc
    34141 atcacgcggg aaaaagtgta gaaccactgg aaggcgagcc cgtttgaata ccggcgctcg
    34201 agctcggctt gcaaggaatg cgtgttcgag aagccgttgt gccggagcgg cgcgttgcag
    34261 caaccgctgg tccagttcgg gttcgcgcgc cgcaggtctt cattggacgg agcggccagg
    34321 ccggtccgaa gctggtaatt gtactgcgat tcgccgtcgt tccagcgcca gcgctgttcg
    34381 aggtcgcttc cgtggttgcc gatgtagctc aaccgcagcg cggtctcctt catcagctcc
    34441 cgctcaaagg tgagattcca ttcctgcatc atgttgtcgc tccaggtgcg gaaatcccac
    34501 ggcatgaacg gctgggcttg tgaagagatg cccgccacgc cctcgatatt cacctgcgct
    34561 ttgccgacga tatcggtagc ggctggcaca ctcttgagtg cgtagttgta gatacttccg
    34621 ttctgggttg caatgtcatt ctcaaaccgc agattgaggg gcgcgctgac ccgagcgctt
    34681 tggaggattt gcgagagcgg catcgtccag tagtagacgc cgtaaccgcc gcgaagcacc
    34741 cacttgtcgg acatgcggaa cgcggcgccc actcgcggag cgaagttgtt gttgtccgcc
    34801 ggcaacaagc tgccgggcaa tccggcttcg ttggccgtct tccaggttac acctcgcttg
    34861 gcccaggatg cgagtacggc cggcgggatg ccgggaatat cttccatccg cgtgtcgtgg
    34921 ggcgtgacga cctccatctt ccccacatag ttctggatgt ccaggttcac cagccggttg
    34981 tacttctcct tgtaaaccgt ccacttgtcc cagcggagac caaggtccac ggtcaacctg
    35041 gggctcagct tccagctatc ttgaacatac agcccgatct ccttctgctg gaagtagaag
    35101 aaaccccggt tgtattggtt ggacaggtac gttggtaacc cgaggagaat actcccgaag
    35161 cccgtgccgg tataagaaac agcctgctgg gccgcggggt cgtagagaga ggtccagtcg
    35221 ccccagaagt aatgcattcc ctgagcttgc tgcaactcgc gggcgttgtt gtactcggag
    35281 cgccccttaa aaccgagctt gactgagtgc gtgccgcgga tccaggtgac gttgtcctcg
    35341 atctggtaag cggtgaggtt ctgtttctgg gggttgtcgg cgtcccagca ccagtcgaag
    35401 aacgggcccg cacacatcgt cggccagccg gtctctccga aggggttggg taaaccgagc
    35461 ttgtcggccc agttggtgct gtccgccaag gtgcccgagc tgttggcgtt ccggtttgcc
    35521 gaggcctgga actcgttcag gaaggtcggc gtgaaaacgt ggttccaacg ggagaacgcc
    35581 gagtagatcg tcgtgtcgct gcgcccggtg ccgccggcat tcgacatgcc cggcggtgga
    35641 tagccgtact gtcctccgta aactttgcgc ggccgtgtgc catgcgtgaa ccggccggag
    35701 aggttgtcct tctccgaaaa gacgtggtct ccctttacgg tgtaggtggt gatgtccaat
    35761 gtcaccggat aataggtctc gaagttggct tcgatccaag cgttgccgcc cgcgttggga
    35821 ccggccggtg cggccgaaac gctcttcatt acgctggcga agggatggat catgttgctc
    35881 gggattctgt tgccgttgaa cggcagccgg gtgccgtcgg ccatggtcga aaacggattg
    35941 tagatggtga tcggcgtgtt gtcgtaatcc accgccgtgc tgaagtcgcc gccccagatt
    36001 gcgtccgtgg gtgcctgggt gcgagcatag cgagcctggc gctccctctg cccttcgtag
    36061 gcggcgaacc agaatgtctt gttcttgatg atcgcaccgc cggcggacgc gccgaactcg
    36121 ttccggatgt attgcggcgg cctggagaag aaatcctgcc tctgtcgcgc gcggagcccg
    36181 ccgaaattgt tgcgatgcgt ccagaacacg ctcccatgca attcgttcgt gccgctcttg
    36241 gtgacgagcg tgacggttgc cgggcgggag tactgggcgc ccgagccggt ggtctcaacc
    36301 cggtattcct ggatgatgtc gaggccgggc tggacgggac gcataccacc gccgaagcgg
    36361 tccatgatcg agatgccgtc cagcagcatg tccgccgagc cgaccttcat cccgttgatg
    36421 cgcgggctgc caccgccctc gacgcccggg gtgaggtcaa acaggttgct gacaaccctt
    36481 ccgttcagcg gaagttgctc gatgcggagg cggtccttga cgtcattcac ctccatgcct
    36541 tcggtggtca cgacgggagc ggcgcccgtc acttccacgg tggcctccag tgcgccgact
    36601 tccattgtcg tatcgactac caccgtctgg ccgacttcca ctcgcagggt cccggcccat
    36661 ttcttgaagc cggaagcctc gactgccagc gtgtacggac cgataggcac actggcgaaa
    36721 tagtaaatcc cgacggcgtt cgtttccgcc gctctggcga cgcctgtcgc cgtgtgagtg
    36781 aggatgacct tcgcgtttgg tatgaccgcc ttcgtggcgt cctgaaccgt tccacggacg
    36841 acgccgagac cggtctggcc cataacaggg acaaccacca acacgagcgt gaggatcagc
    36901 aagacacact tgcttctgaa ggaaagcgaa actggcatgc aatctccctg tgcactgaat
    36961 gtgctggttg gagtatactt ccccaatcct attcaggtca agagatctta atactttaaa
    37021 ctctcggttt gctgcgttca gcaactactt ccccagcctc gcaatccgga tgttgcggaa
    37081 gtccaggttc gtcgtcgtga aaatccgacg ggcacgtcat tacaggtgcc aaagcccggc
    37141 cggagttcgg atggtctaca ctggcggaag actgtcctgt gcgcctggat tccatttcgc
    37201 accgttccgg acgccgctgg tggtgggcct tcgccctcct gatcgccact cctgcgttag
    37261 cgctagcatt actggggcta cgggtagcgc agcttgaaag ccttcagcgc gcgcagcaga
    37321 ctcgggagga gcagtcgcag ttggggcggc tgacggatac cacaatctcc tcaatgttga
    37381 ccgtactgaa gagcgaactg gcgcggactg agacgtcgct ggcagcagct cgggattaca
    37441 cgccctttgt tttggagccc ggcggaagat tggtatttcc gcgggaaaag acttattttc
    37501 cggaccccag tgtgctggac tcccactccg gaccgcaatg gtcccaggca accagccaag
    37561 agatcgagcg tgcccagatc gccgatcagc agcgcgccgg cgaggcttct tcgctgtttc
    37621 accacatcgg caagactgag ccgaggctgc gtgcttggtc tgaactggca ctggtgcgac
    37681 ggcagttcga atcgggcgac gtcagcgcac tgaccgctct tgccaaccct tcctggactc
    37741 attcggaagg catgacaccg agcggtttgc ctgtggcttt gttggcagcc acctatgcgg
    37801 aaggccttcc cccagatcag cgctctcgct tcgttccgct actgaaggcc acactggaag
    37861 ccctgcgcgc gggacggtgg tggttcagca ttgaggagcg ccgtttctat gatgaggaac
    37921 tgcggcggtt gatcgctcta gctggctcac gaccgccagc cgatgacgcg cggctggccg
    37981 agtgggcatc gattgagcgt cttgcgcggc gcatccgccc ggcaggggat gctcgccctt
    38041 ctgatcccga cccggcagcg ccattcctgc tgctgtggtc ccatagccca gacggaactt
    38101 ggctgggcgc ggcggtctcg cgtgaccgtc tcaccggctt ggtcgacaga gccttgcaac
    38161 ctttgtttac aactcggtct ttcgcggtgg ccatccgcca tttgaaaggt cccatcatct
    38221 ggagccggcc tcttcagagc acactcgccg gggccgctgc tccgctccag gcggtggagg
    38281 actgggaact cgtctttagc cggcccaccg agggcggtgc attcgatagg aggcgtctgc
    38341 tctggtacgg attcacattg gtcgtggtta tgacgatggc tgtcggattg gccgtgactc
    38401 tgtttgtggt caaacgcgag gttgagctga accagttgca gtctgacttt gtcgctgcgg
    38461 tgacacacga attcaaaagc ccgatcacca gcatacgttt gctggtggaa cggctcagca
    38521 gcggacggct gcgaacgccc gccgctgccg gcgaatatca agatgcgatt caccgcgaaa
    38581 cggatcggct ggaacggctg gtcaaccggg tgctcgaatc gcagaagatc cagtcgggcg
    38641 agaagcgata caacttggtt cccggctcgg tcgtggagct ggcggaagca gcgatctgga
    38701 ggctgcggcc gcaggcggac gccaagaata tcggcgtcga tctggaactc gccggcgaga
    38761 ttccccagat tcagatcgac aagacagccg tcgccgacgc cctggacaac ctggtggata
    38821 acgccatcaa gtattcgccg gagggcgccc gtgtccttgt tcggatccag agacgtgagc
    38881 gttttctgtg cgtcgaggtt caggatcatg ggatcggaat cgaccccgaa gacctgcccc
    38941 gcatcttcga caggttctac cggggtcgcc gcggcgatca gcacgacgtg cacggcaccg
    39001 ggctcgggct tgcgctggtc aaagccgccg cagaagggca tggaggaagc gtcgaagtga
    39061 ccagcgctcc cggtcagggc gcccggttct gcctgcgcct tccgctggag agctgatggc
    39121 gccccgcatt ctagtcgtgg atgacgagcc gtccatcatt cttgccgtga aagatgagat
    39181 ggagttcgag ggtttcgtcg tggattcggc cgcggacgga ccgagcggtc tcaagaaagc
    39241 ccgcgagtcg cggccggacg tacttctggt ggacctgatg ctgcctggat taaacggttt
    39301 cgagatctgc cgtcaactgc gtgccgactt gcccgacttg tggatcatcg tgctgaccgc
    39361 ccgaggccag gaggtcgatc gggtgaccgg tttcgaagcc ggcgccgacg actatatcgt
    39421 caagcctttc tccctgcggg agctcgtggg gcgtgtgaaa gtcgggctga ggcggcgcaa
    39481 ggccaccgcg ccggcattgt tgcgcttcgg cgatctcgtc atcgatcccg gcgcccatcg
    39541 ggtcaccaaa gccg
//
LOCUS       DATIFQ010000130         3567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1958532, whole genome shotgun sequence.
ACCESSION   DATIFQ010000130 DATIFQ010000000
VERSION     DATIFQ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958532"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VLE22_05950"
     CDS             complement(<1..465)
                     /locus_tag="VLE22_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13981.1"
                     /translation="MAFSRREFMTMAGAAALAAKPYFGLHPFIEANPKAVFIRRTRVR
                     EKMDSAAKRTEGLRLAREIFVPMDGPGVPLTHRIVLKPNVISVKSRNRPAAENWGTGT
                     DPDFYEGIVLGLKEIGLRKFHFIEANLFHMWNYRGFVDINERLGVAMNDPELR"
     gene            complement(487..1983)
                     /locus_tag="VLE22_05955"
     CDS             complement(487..1983)
                     /locus_tag="VLE22_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13982.1"
                     /translation="MKPPVLLRCLLAAFVLPAVLGAAIDLENELVVRWPRVDDESAKL
                     LKELGVKVVIVPRSENQPAGEVHRVCRRAGLTPALEIGHFERWEEIEKEFAQAMTDGF
                     RVVVFEAIENSADRIRELAATHGDAGVLALLKPEWAGWQVAPAIAVLNDGRWPGLEAR
                     DPTVASASEAPWINANCHLFGYLRGMFPERPAALSYLPDEKAGVKKSQVVHFESVELS
                     LAEAMAGGGNVLLAFPESYRTALAAKETKALEAWRSLRETAVLLRAHRDRFRQPLSQD
                     VVVAGGDLDQTGEILNMFFRRNLSPRSRPVSRLSPASVSAGRVLVVVNIAPPPPPARA
                     AITTFAKNGGVVLLAPAANGDKAWWTSADPPAAHESERDVYAVGKGRVAVYNEPVIDS
                     AEFAWDVIDSLGSVNLDLRIWNAPTVIGLVRRQKDGAAVVALLNYGSPLTMQEDDEIL
                     MRIRGTFTRVELSLPGKPAPAPLKATKRGGTTEVEVRGLERIAVVSFQ"
     gene            complement(1980..3404)
                     /locus_tag="VLE22_05960"
     CDS             complement(1980..3404)
                     /locus_tag="VLE22_05960"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HSB13983.1"
                     /translation="MLRSVLSLLGCAFSLCASDLMIGRGVVKITPPAGTPMGGGFYIQ
                     VSTGVHDDLYCKALAFAEGASKAAIVSCDVESLHRPTVEAARALIDRRTGIPGRNVML
                     TATHSHSGPEMTPMVIDGATGEAGRLIREFHAGLPGKIAEALRLAEANFVPTRVWTGA
                     GHEDSVSFNRRFLLKNGNVQMNPGQLNPEIVRAMGPIDPSVSVVYFDTPDGKPLATAV
                     NFALHATTFGELDFSADYPGVLAARLGEAKGSEMLTLFTNGCAGNINQVDVTSRHHPF
                     GPGESARVGTILAATVLKTYKQMDAAGSGPLQVRSEIVSLPPLSYGEADVKKARQVVE
                     LSRKQELDFLDLVQANRILNVMETHGGKPIPAEVQVIAIGDKVAWVGLPGEVFVELGL
                     AIKLASPFRLTLVSELANGMLDYLPDRKAHEEGGYEVTTARCAEGCGEALVDTARRLL
                     VETYPVKYPSGARARGGMDGGTRR"
BASE COUNT          634 a         1219 c         1050 g          664 t
ORIGIN      
        1 ccgcaactcg ggatcgttca tggccacacc gagccgttcg ttgatgtcca caaacccgcg
       61 gtagttccac atgtggaaca ggttcgcctc gatgaaatgg aacttccgga gcccgatctc
      121 cttcaggccg agaacgatgc cctcatagaa gtccggatcc gtgcccgtcc cccagttttc
      181 ggcggcgggc ctgttccggc tcttcacgct gatgacgttc ggcttgagga cgatccggtg
      241 cgtcaaggga acgcccgggc cgtccatggg gacaaagatt tctcgcgcca gtcgcaggcc
      301 ctccgtgcgc ttggcggcgg agtccatctt ttccctcacg cgggttcggc gaatgaaaac
      361 cgccttcggg ttcgcctcga tgaacgggtg cagtccgaaa tacggctttg cggccagcgc
      421 cgcggcgccg gccatggtca tgaattctcg tcgcgaaaac gccatcgcat cacctcgctt
      481 gggagttcac tggaacgaga ccaccgcgat ccgctcaaga ccgcggactt cgacttctgt
      541 tgtaccacct cgcttggtcg ccttgagcgg cgccggcgcg ggtttccccg gtagcgaaag
      601 ttccactctc gtgaacgtgc cgcggatccg catcaggatc tcgtcgtcct cctgcatcgt
      661 caacggggag ccgtagttca acagcgccac gacggcggcg ccgtccttct gccgccgcac
      721 cagtccgatc accgtaggag cgttccatat ccgcagatcc aggttcacag aaccgaggct
      781 gtcgatgaca tcccaggcga actccgcgct atcgatgact ggttcgttgt aaaccgcgac
      841 tcgtcccttt ccgaccgcgt agacgtcgcg ctcggattcg tgggcagcgg gcgggtcagc
      901 ggatgtccac cacgctttat cgccgttcgc ggcaggcgcg agcaacacga cgccgccatt
      961 tttcgcgaat gtcgtgatgg cggctcgcgc gggcggcggg ggcggcgcaa tgtttaccac
     1021 gacgagcacc cgaccggcgg acacggacgc cggcgaaagc ctgctcaccg gccgggaccg
     1081 agggctgagg ttgcgccgga agaacatgtt caggatctcg cccgtctgat cgaggtctcc
     1141 gcccgccaca acgacgtctt gggaaagggg ctgtcggaac cggtcgcggt gggcccgcag
     1201 cagcacggcg gtttcacgga gggaacgcca agcttccagc gcctttgtct ctttcgcagc
     1261 gagcgcggtg cggtaacttt cggggaatgc caggagcacg tttcctcctc ctgccatcgc
     1321 ctccgctagc gacagctcca ccgattcaaa gtgcaccacc tgcgacttct tgacgcccgc
     1381 cttctcatcg ggcaggtagg aaagcgcagc cgggcgctca gggaacattc cgcgcaggta
     1441 cccgaagaga tggcagttgg cgttgatcca cggcgcctcg ctggcactgg caacggtggg
     1501 gtcgcgcgcc tcgagtcccg gccaccgtcc gtcgttcagc accgcaattg ccggggcaac
     1561 ctgccaaccg gcccactcgg gcttcaacag cgcaagaact ccggcgtccc cgtgcgtagc
     1621 cgcgagctcc cgtatcctgt ccgcactgtt ctcgatggct tcgaacacca ccactcgaaa
     1681 cccatctgtc atggcctgcg cgaactcctt ctcgatctcc tcccaccgct cgaagtggcc
     1741 gatttccagg gcaggcgtga ggccagcccg ccggcacaca cggtgtacct ccccggccgg
     1801 ctgattctct gaccgcggca caatcaccac cttgacgccg agttccttga gcagcttcgc
     1861 gctctcgtcg tccactcgcg gccatcgcac caccaactcg ttttccaggt cgatggcggc
     1921 cccgagcaca gccggcagca cgaatgccgc aagcagacat cgcagaagca cgggcggctt
     1981 catcggcggg tacctccgtc cattccgcct cttgcacgtg ccccgctcgg gtacttcacc
     2041 ggataggtct ccaccaacag gcggcgagcg gtgtcaacga gcgcctcacc gcacccctcg
     2101 gcgcaacgcg ccgtcgttac ttcgtagccg ccttcctcgt gcgccttccg gtccggcagg
     2161 tagtcgagca tcccgttggc cagttcgctg acgagcgtaa ggcgaaatgg cgacgccagt
     2221 ttgatcgcca gtcccaattc aacgaatacc tcccctggca ggccgaccca tgcgaccttg
     2281 tccccgatgg cgatcacctg cacttccgcc ggaatcggct tcccgccatg cgtctccatc
     2341 acgttcaaga ttctattcgc ctgcacaagg tcaagaaaat ccagttcctg ttttcgggac
     2401 aactcaacca cttggcgagc cttcttcacg tccgcttcgc cataagaaag cggaggcagg
     2461 ctcacgatct cagacctcac ttgaagtggg ccggaaccgg ctgcgtccat ctgcttgtaa
     2521 gttttgagaa cagtggctgc gaggatcgtt ccgacgcgtg ccgattcacc gggcccgaac
     2581 ggatggtgac ggctggtcac atccacttgg ttgatgttcc cagcgcagcc gttcgtgaaa
     2641 agcgtgagca tctccgaacc cttcgcctca ccgagccggg cggccagcac gccgggatag
     2701 tcagccgaga aatcaagctc accgaacgtg gtcgcgtgca gagcgaagtt caccgcggtc
     2761 gccagcggct tgccatccgg agtgtcaaag tagaccaccg aaacggacgg atcgatcggc
     2821 cccatcgccc ggacgatctc cgggttcaac tggcccgggt tcatctgcac gttgccgttc
     2881 ttgagcagga accgccggtt gaaactgacg ctgtcctcgt gccccgcacc cgtccacacc
     2941 cgcgtcggga cgaagtttgc ctcggccagt cgaagggcct ccgcgatctt gccaggcagg
     3001 ccagcgtgaa actcccggat caaccgtccc gcttctcccg tggcgccgtc aattaccatc
     3061 ggcgtcattt ccggcccgga gtgcgagtgg gtggccgtca gcatgacatt tcgccccggg
     3121 atgccagtcc ggcggtcgat cagcgcgcgg gccgcctcca ctgtcgggcg gtgcaggctt
     3181 tccacgtcgc agctcacgat cgcggctttc gatgcacctt ccgcgaaggc tagcgccttg
     3241 cagtagagat cgtcgtggac gccggtgctg acctggatat agaacccgcc gcccatcgga
     3301 gtacccgccg gcggtgtgat cttcaccacg ccgcgtccga tcatgaggtc ggaggcacac
     3361 aggctgaagg cgcaccccag cagagacaag acgctccgaa gcatgccaag atgctagcac
     3421 acggcgctat ttcgcttgcc acctttgacg agcgggagca taatcaacca ggatcgtctc
     3481 ccgcgccggc gtatggtccg gctccgtgca gagggatccg atgtcacgga ggtggcaatg
     3541 ttccactctc tttcggggtt caaggcc
//
LOCUS       DATIFQ010000131         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1766927, whole genome shotgun sequence.
ACCESSION   DATIFQ010000131 DATIFQ010000000
VERSION     DATIFQ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766927"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2658
                     /locus_tag="VLE22_05965"
     CDS             <1..2658
                     /locus_tag="VLE22_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSB13984.1"
                     /translation="KLGGGGMGVVYKAEDTRLGRTVALKFLTQGHATDPQAFERFQRE
                     ARVASALNHPHICTLYDIGEWEGAPFLVMEHLEGQTLKRRIAGKALKLEEVLIAAIQI
                     ADALEAAHAKGIVHRDIKPANIFLTKRGDVKVLDFGLAKLAGDQRKERVLPDGSGTLP
                     MSEESLTVQGTTLGTVAYMSPEQAEGKDLDVRSDIFSFGAVLYEMLTGRPAFRRDSTA
                     STLAAILKEEPKPVGELVRGLPAELERILRQCLRKAVEHRFQYMADVKVELEEVRGEL
                     PSMGTQPQLPVPSGTPGRRLLWIALLLAVTGLGVRFWPFGMGTGELGLQAVLLTGYPG
                     SEDSPSFSPDGNQVAFEWDGPQQDNRDIYVKQVGNETALRLTTDSAPDTTPAYSPDGL
                     SIAFLRWMPNGKKAVMLVPAIGGPESKLAETRCCAYSNTRVSPGLAWSPDGTWLAVMD
                     GESEWGPPSLYLLSVESGEKRRLTAPPPGAGDGTPAFSPDGRHLAFGRNSIGDVSELY
                     VLDFAEDFSPRGEARQLTSLKLSSFSPAWTADGREIVFSSTSLVGAFYELWRVPVTRS
                     GVPRRLQFPGEQGVRPAISRAGNRLAYVRRYSDRNIYRLEVPSARRGVSAPLSLISST
                     GDDYLPQYSPDGRRIAYVSARSGRPEIWVCASDGSNAVPLTSMRATVVGPPQWSPDGE
                     QIVFDSNLEGKYEVYVIGARGGRPRRLTNHPASNDLGSWSRDGRWVYFASNRTGRSET
                     YRIPAGGGQAEQVTRNGGFSAFESPDGSSVYFWRGGGVYRIRKTGGEETEILAEKSIG
                     PFGFALGRKGIYYFREAYWEDAQAPPEGIAWRQLQYLPFDGGTPATITAVKPDPRFAS
                     VSVSPDERFILYGQDDQSGSDLMLVENFR"
BASE COUNT          500 a          825 c          889 g          482 t
ORIGIN      
        1 aagctcggcg gcgggggcat gggtgtcgtc tacaaggccg aggacacccg gctcggccgc
       61 accgtcgccc tgaagttcct gacccagggc catgccacgg acccgcaggc cttcgaacgc
      121 ttccagcgcg aggctagggt cgcctccgcc ttgaaccatc cccatatctg cacgctttat
      181 gacatcggcg aatgggaagg cgctccgttc ctggtcatgg aacacctgga agggcagacg
      241 ctgaagcggc gcatcgccgg gaaggcgttg aagcttgagg aggtgctgat tgccgcgatc
      301 cagatcgccg acgcgctgga ggcggctcac gccaagggca tcgtccaccg cgacatcaag
      361 ccggccaaca tcttcctcac caaacgcggc gatgtgaagg ttttggactt cggactggca
      421 aagctggccg gcgaccagcg caaggagcga gtgctgccgg acggctcggg gactctgccg
      481 atgagcgagg aatcgctcac cgtccagggc actacgttgg gcacggtggc ctacatgtcg
      541 ccggagcagg ccgagggcaa ggacctggac gtccgttcgg acatcttcag cttcggcgca
      601 gtgctatacg agatgctgac cggccgcccc gccttccggc gtgactccac ggcttccacg
      661 ctggcggcga ttctcaaaga ggaaccgaaa ccggtgggcg aacttgtccg cggtctgccg
      721 gccgaactgg agcgcatact ccggcaatgc ctgcggaaag cggtggaaca ccgcttccag
      781 tacatggccg atgtgaaggt ggagttggag gaagtgcggg gggagcttcc gtccatggga
      841 acccagccgc agttgcccgt tccatccggc accccggggc gccggctgct ttggattgca
      901 ctgctgctcg ccgtgacggg gctgggggta cgattctggc cgttcggcat gggtacgggg
      961 gaactgggtc ttcaagcagt cctgctgaca ggctacccgg gcagcgagga cagcccgagc
     1021 ttctctcccg atggaaatca ggtcgcattc gagtgggacg ggccgcagca agacaaccgg
     1081 gacatctacg tcaagcaggt cgggaacgaa accgcattgc gcctcacgac ggactccgcc
     1141 ccggacacca ccccggccta ttccccagat ggactgagca tcgcgtttct gcgctggatg
     1201 ccgaatggaa agaaagccgt catgctggtg ccggccatcg ggggaccgga gagcaagctg
     1261 gcagagacgc gctgttgcgc gtacagtaat actagggtct caccggggct tgcttggtcg
     1321 ccggacggca cgtggctcgc cgtcatggac ggcgaatccg agtgggggcc accctccctc
     1381 tatctgctct ccgttgagag cggtgagaag cggagactca ccgccccgcc gcccggcgcc
     1441 ggcgatggta cgcctgcatt ctcacccgac ggacgccacc tggccttcgg ccgcaattcc
     1501 attggcgacg tcagcgagct gtatgtgctc gattttgccg aagacttcag tccgcgagga
     1561 gaagcgcggc agctcacatc tttgaagctg tcttccttta gcccggcctg gacggcggat
     1621 ggccgcgaaa tcgtcttctc ttctacctcc ctcgtgggag cattctatga gctgtggaga
     1681 gtgccggtga cacgatccgg cgtgccgcgg cgcttgcagt ttccaggaga acagggcgtc
     1741 cgcccggcga tttcccgcgc aggcaaccgt cttgcctatg tgcggcgata ttcggacagg
     1801 aacatctacc gcctggaagt accgtcggcg agacgcggtg tgtccgcgcc gctgagcctg
     1861 atctcctcga cgggggatga ttacctgcct cagtattcgc cggacgggag acgaatcgcc
     1921 tatgtttcgg cccgttcggg gagaccggag atctgggttt gcgcaagcga cggatcgaat
     1981 gctgtcccgt tgacctccat gagagcgacg gtcgtcgggc ctccgcagtg gtcacccgat
     2041 ggcgagcaga tcgtcttcga ctctaacctg gagggcaagt atgaggtcta cgtgatcggc
     2101 gcgaggggcg gcaggccgcg ccgcttgacg aaccaccccg cgagcaacga cttggggagc
     2161 tggtccagag atgggcgctg ggtctacttc gcttcgaacc gcaccggacg atctgagact
     2221 tacagaatac ccgcgggggg cgggcaagct gagcaggtca cgcgaaacgg aggattttcc
     2281 gctttcgagt ctcccgacgg ctctagcgtc tatttttgga gaggcggtgg cgtatatcgg
     2341 atccgaaaga ccggcggcga agagaccgaa atactcgcgg agaagtctat cggaccgttc
     2401 ggcttcgcgc tgggcagaaa gggcatctat tacttccgtg aggcctactg ggaagacgcc
     2461 caggcccccc ccgaggggat agcctggcgt cagcttcagt atctgccttt cgacggaggc
     2521 acgccggcga caatcacggc cgtgaaacca gacccccggt ttgcaagtgt gtctgtgtcc
     2581 cccgatgaac gcttcatcct gtacggccag gatgaccaat ccggcagcga cctgatgctg
     2641 gtggagaatt tccggtgagg gtctcggagc agacccgact cgacagccgc gatact
//
LOCUS       DATIFQ010000132         3196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_426669, whole genome shotgun sequence.
ACCESSION   DATIFQ010000132 DATIFQ010000000
VERSION     DATIFQ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426669"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VLE22_05970"
     CDS             <1..201
                     /locus_tag="VLE22_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13985.1"
                     /translation="YEYFWLLAQKAGGRGQADEAVNGIVSDFVECNCGVEPLGTPGMW
                     KHNPDEWERARISLGELIDKTR"
     gene            complement(256..792)
                     /locus_tag="VLE22_05975"
     CDS             complement(256..792)
                     /locus_tag="VLE22_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSB13986.1"
                     /translation="MMKRSSFRKLLVVAAVALIAAPLLAQGATGNGQLEQKVRHELLM
                     LPYYGVFDNLAFRVEGNTVVLLGQVRRPTLRPTAERVVKQIEGVSAVDNRIEVLPLSF
                     HDDRVRLGVLRAVYGNSALYRYNLGAIAPIRIVVKNGNVTLEGVVANEMDRNIAFLAA
                     NGVPGVFSVKNNLHVNNG"
     gene            complement(865..1437)
                     /locus_tag="VLE22_05980"
     CDS             complement(865..1437)
                     /locus_tag="VLE22_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13987.1"
                     /translation="MYNPLVDQLGTLGFIATDAAGGHWIVSCYHVLCRTNGSAFPDGE
                     PVYQPLDGEPDALVARLSADRADPVVDCAAARVVDGVLTAAEIFGLGKPGIPAVPVTG
                     MRVVKSGVATGITEGVIREVDGDRVTIAVPDGFPVQYELTHGGDSGALWMERDTRAPV
                     ALHTRGNDTGEELAFGVSILKIINSLGVFL"
     gene            complement(1502..2398)
                     /locus_tag="VLE22_05985"
     CDS             complement(1502..2398)
                     /locus_tag="VLE22_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HSB13988.1"
                     /translation="MKARHIALVVAAALVVLAGCGHSALRTRAARYSVRTVREAGLSP
                     DDQKRADRNCVFGMPRINPKWDFGPTRFVFRDGYVLQHSSIDKVPLWVCEDVTTQQLT
                     GAAKRGNKFAPDPLLPPKERAELADYKGSGYDRGHQAPAGNQAQDQRLKDETFFLSNM
                     APQKPKLNQQAWRELEDLTRKWTEKRGQTWQITGGMFYDPAEEDEKTADGLIEYTTIG
                     KDNVGVPTHFYKIVAGKGPSGDWEAIAFVMENKAYPRPFHFEQFIQSIDWIEERTGLD
                     FMPDLDPVEERRLERKPSPMWQ"
     gene            complement(2453..2668)
                     /locus_tag="VLE22_05990"
     CDS             complement(2453..2668)
                     /locus_tag="VLE22_05990"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HSB13989.1"
                     /translation="MTVDRYLRMIAGAFVLASMALGHWVNPYWYLFTAFVGLNLFQSA
                     FTDWCPMITFLRKLGVKTCADELPAKG"
     gene            2917..>3196
                     /locus_tag="VLE22_05995"
     CDS             2917..>3196
                     /locus_tag="VLE22_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13990.1"
                     /translation="MSRLNALLQWSVAAVAFAGAHPVCFGGPVAPVTLDFLLRDASAI
                     VVATVDGSVVNGAATGNLIVERALRGELRPGSAVQLTWTGRQTVQPMSR"
BASE COUNT          609 a          960 c         1001 g          626 t
ORIGIN      
        1 tacgaatatt tttggctgct ggctcaaaag gcagggggac gcgggcaggc ggacgaagca
       61 gtcaatggca tcgtgagcga cttcgtggaa tgcaactgcg gcgtcgagcc gttgggaacg
      121 cctgggatgt ggaagcacaa cccggacgag tgggagcggg cgcgcatcag ccttggggaa
      181 ctgatcgaca aaaccaggta aaaaaatggc ccgacccaag gagggccagg ccgctcgttt
      241 gcagaatccg aaaactcaac cgttgttgac gtgcaggttg ttcttcaccg agaacacccc
      301 cgggacgcca ttcgccgcga ggaatgcgat gttccggtcc atctcattgg ccacaacgcc
      361 ctccagtgtg acgttgccgt tcttcacaac aattcggatc ggcgcgatcg caccgagatt
      421 gtaccggtag agcgcactgt tcccatagac ggctcgaagg acccccagcc ggacccggtc
      481 gtcgtggaac gacagcggca gaacttcgat gcggttgtcg accgccgaca cgccttcgat
      541 ctgcttcact acccgttcgg cagtcggccg aagcgtcggc cgcctcacct gtcccaacag
      601 gaccaccgta ttgccctcca cgcgaaaggc aagattgtca aaaactccgt agtaggggag
      661 catcagcaac tcgtgacgga ccttctgctc cagttggccg ttgccggtcg ccccttgagc
      721 cagcagcgga gcggctatca acgccaccgc cgcgaccaca agcaatttcc gaaagctact
      781 gcgtttcatc atcccctcct tctactcatt gagatgcact gtgcgaaggg gccgttgcag
      841 gggtgacaga ccttaacaga aactttacag aaatacgccc aagctattga taatcttcag
      901 aatactaact ccaaaggcca gctcttcccc ggtatcgttg ccgcgggtgt gaagggccac
      961 gggggcgcgg gtgtcgcgtt ccatccagag cgcaccggag tccccgccgt gagtcagttc
     1021 atattgaacc gggaagccgt ccggtacggc gatggtcaca cggtcgccat ccacttcgcg
     1081 aatcacgccc tccgtgatcc cggtggcgac accggacttg acgacacgca tcccggtgac
     1141 aggaacggcg gggatgccgg gtttcccgag tccgaagatt tcggcggcag tgagcacgcc
     1201 gtctacgacg cgcgctgcgg cgcaatcgac aaccggatcc gcgcggtccg ccgacagccg
     1261 ggcgacgagg gcgtcaggct cgccgtcaag gggctggtag acgggctcgc cgtccggaaa
     1321 agcgctgccg ttcgtgcggc aaagcacgtg gtagcagctc acgatccaat ggcctccggc
     1381 agcgtccgtg gcgatgaagc cgagggtgcc gagttggtcc accagcgggt tgtacacggc
     1441 gatcccaccg gtgagcggcc ggtatttcag ggcgtggttt cgaacgatga tggcgggcct
     1501 cctactgcca catcggcgag ggcttccgct cgaggcgccg ctcctcgacc gggtcgagat
     1561 ccggcatgaa gtccagcccg gtacgctctt cgatccagtc gatcgactgg atgaattgct
     1621 cgaagtggaa gggccgggga taggctttgt tctccatcac gaacgcgatg gcttcccaat
     1681 cgccggacgg gcctttgccg gccacgatct tgtagaagtg agtcggcacg ccgacattgt
     1741 ctttgccgat cgtcgtgtac tcgatcagac cgtcggccgt tttctcgtct tcctcggcgg
     1801 gatcatagaa cattccgccc gtgatctgcc acgtctggcc gcgcttttct gtccatttcc
     1861 gggtcaggtc ttcgagttcg cgccacgcct gctggttcag ttttggtttc tgcggagcca
     1921 tgttggagag aaagaaagtt tcgtccttga gccgctgatc ctgggcctgg ttgccggcag
     1981 gcgcctggtg gccgcgatcg tagcctgagc ctttatagtc cgccagctcg gcgcgctcct
     2041 tcggggggag gagcgggtcc ggggcgaact tgttgccccg tttcgcggcg ccggtcaatt
     2101 gttgcgtggt gacgtcttcg cagacccaca gcggcacctt gtcgatcgag ctgtgttgta
     2161 acacgtagcc gtcgcggaag acgaaccgag tgggcccgaa gtcccatttg gggttgatcc
     2221 gcggcatgcc gaaaacgcag ttccggtcgg cgcgtttttg gtcgtcgggg ctcagcccgg
     2281 cttcacggac ggtccggacg gaatatcggg cagcgcgtgt gcgaagcgcg gagtgtccgc
     2341 atccggcgag gacaacgagc gcggccgcga cgactaacgc aatatgacga gctttcatcg
     2401 gccctccgat ccaatctata gtgccggagg gccgcaccgc gttacaaggg actcagcctt
     2461 tggccggcag ttcgtcggcg caggtcttca cgccgagctt cctcagaaac gtgatcatcg
     2521 ggcaccagtc ggtgaaggcc gactgaaaca ggttcagccc gacgaaggcg gtgaaaaggt
     2581 accagtaagg gttcacccag tggcccagcg ccatgctggc cagaacgaac gccccggcga
     2641 tcatgcgaag gtaacgatct acagtcatcg tcaatctcct cttacgaacc cagatgcgga
     2701 acagcggaaa aggtgacggg gcagcctcag ctccccgcca ctttccgctt gttcacaatg
     2761 tagtacacaa tcgggacggt catgcgggag agggccagcg aggcgatctc gccggccatc
     2821 agggcaatcg tgccctgaga ttcgcggttt ccagcctaag atcctgaaac tgctagaccg
     2881 ttcggtgcac catatatttc tggaggtgcc cccgtattgt ccaggctcaa tgcgttattg
     2941 cagtggagtg tggcggcggt tgcgtttgcc ggcgcccatc ccgtttgttt tgggggcccg
     3001 gttgcgccag ttactttgga ctttcttctc agagacgcaa gcgcgatcgt ggttgccact
     3061 gtcgacggga gcgtcgtcaa tggggcagct accggcaatc ttatcgtcga gcgagcgttg
     3121 cggggtgaat tgcggcccgg ctcggcggtg caactgacct ggactggacg ccagacggtt
     3181 caaccgatgt ctcgac
//
LOCUS       DATIFQ010000133        24953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_43223, whole genome shotgun sequence.
ACCESSION   DATIFQ010000133 DATIFQ010000000
VERSION     DATIFQ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24953
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43223"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VLE22_06000"
     CDS             complement(<1..1449)
                     /locus_tag="VLE22_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13991.1"
                     /translation="MRAIISAVVVLYSILPALAQSPDKVAPAINDIELWQQVGQQPYE
                     FTWVQRAEHPKTLVDFEDLNGWKLEMFDGAKGELRRSREQQMWGQYVAKFLYSGTSEK
                     SRVVARPPKPIPIPDKFDSIELWGYGNRWSWVPDPTTPAADVSILVTDSRGKEHTIQL
                     TNIQWKQWWLIHRKVPAATLKQIVWPATFTGIEISKGKNTDLRYFFCDSLAFETEELK
                     PLTLQPQPKRNLKPFRGQIAGMNIGEGTLPFPTREETILPLNFEKEFQVTMRQVEKNR
                     FELQYKGKDGTVRYEYRPSAGNMSELTASVNGGASFRPMDGGGIRFAGTPEGKISEGR
                     LVSAAVNGDVIEAKFRHGSQIVDYNLRLWQKSIVLDVWCDGGQATEFRFGRVAGVANP
                     KLINVPYITYGQTNPRVLMSGNADKPMFTSIWFDWYRTNASEPFYAKDPKATADGAEI
                     NGGMRYIAKTNGERNNLYERIFVTASPFYEETL"
     gene            complement(1612..5019)
                     /locus_tag="VLE22_06005"
     CDS             complement(1612..5019)
                     /locus_tag="VLE22_06005"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB13992.1"
                     /translation="MTRLSSENLYLRVLSVLSLLLLLPILGFAQGNITGLVTDSTQAV
                     IPDVEITITHTATNVATTARSGPTGVYTLMRVPIGTYTLVAKKDGFRAYQQTQIPVLE
                     GDTLRLDITMQLGQVTETVNVTGAAPLVQSENAQVGMTITSSQFTELPLTLGGGIRNP
                     SSFIYLQPGVTPGSTWTKQINGVSSFSDQVYYEGISLSRGDLANDAEVNPSVDAIAEF
                     KLIANNYTAEYTHALGGVTTFNMKSGTNDLHGNAFWLNSVEAYNARNFFQRSKPKYRQ
                     NNWGFTVGGPILLPKIYDGRNKSFFFFSFDQFYLRAPGSPGLGTVPTAKMLEGDFSEW
                     VDSGRGLVYNPSSQRTVDGKVIRDPFENNVVPKSMWSRISTQMVGMHPQPTYPGLTSN
                     YQLETGAPSTDQRSSGFKIDHLFTEAHRVSGMFNFTDRPAVKCPNHNNSGAASLAGDL
                     ECHNIQRVTTRIVRLNYDWMVSPTVLNRFSAGLSRFRNPNFSVHYGKGWPEKLGLTGL
                     GGDLFPWVDFNHDYAGYGDTIASDNYFTNFTFLDTLSVMRSNHTLKFGVEVQRHRNNF
                     RDFGTTGGNWQFNQLSTGLPGVSKSGNAFASFLLGDVYSASAFFPFLQYGNRNGYYSP
                     WVNDDWKVTPKLTLNLGLRWEIQPGFTDPNNRLSYMDPTAPNTPLGGIPGAYVYASDG
                     AFSSTGNTHWKDFAPRFGFAYRVTDKTVIRGGFGIFYAQVITQGTGVNGLREGYNVSA
                     SFSSPDLGVTPAMNWDNGFRQDFAHPPQLIPGLKNTQSATLVDRDRSTIVSYTHQLNF
                     IIERQVGQSMSFTIGYVGNGGRRLSKGINWNQNETKYLSLGSLLSKNILDPAVVAAGF
                     QEPFAGFATLWGSRGTLAQALKRWPQFTSVGQAGAFFGSSNYHSLQISGLRRMSKGLM
                     FTLAYTFSKSMNDTEVYGYGAGTMDYFNRKLDKSLASLDQPHTLSLSYIYELPFGPGQ
                     KYLAQGVTSKILGGWKITGMQIYASGTPLGFNINNNLPIGNASQRPDIVSSNLRSSVS
                     ANAFDPAKDLWLNPGAFAIPAAYTFGNAPRNTWARSPAFMQESLGLLKDTKWGERFNW
                     QLRVESSNPLNRVVFGGPVSNFSSGSFGKITSARGSRSLTLGTKFYF"
     gene            complement(5341..7401)
                     /locus_tag="VLE22_06010"
     CDS             complement(5341..7401)
                     /locus_tag="VLE22_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB13993.1"
                     /translation="MALPVQSKRSYFPRLPNALACACLLATAILSSCKHSESSGARTA
                     STTPLRPLNLVVVTIDTLRADHLRCYGYPKIETASVDRIAQDGVLFENAVTQTPLTPP
                     SHASMFTGLYPTAHHVRGNGGFILRPSTTTLATILQQQGWDTAAFISSVSLKRVFGLN
                     QGFAVYDDQMPKAGKGQEFVADAERPAGQTVDRALRWMDAQSGKPFFLWVHVFDPHVP
                     YNPPAPFRNQYRDRPYDGEIAYADHELGRLFDAVSKKSPPGKTLIAVLSDHGESLGDH
                     GEYSHGVFVYDSTLRIAFIMSGPGIPAGLRVKQQARTVDLLPTVLELMGGRPPASVQG
                     VSLTPFFAGKNAATDVSYAETLYPKINMGWAELRGIRTNRWKYILAPKPELYDLSQDP
                     AETNNVIQSHAAEAQKLQAELAAIAGKGTEKVETSTVDQRTLEQLRSLGYVSGVSQPT
                     FDLKGKGVDPKDRLGVLKAIEEADGPTSHLPFPHRIELLRQALKQDAANPTLYYSLGR
                     NCERAGRYAEAIDVYRTAIRNGVESGWLRSRIGDFLVRSGRKTEAIPEYERAIRLDPA
                     DTGTQTNLATAYVETGRLEDAERIFKGIIKIDETHAAAYNGLGVIAIQRQDTAAARSY
                     FEKTVQLDPDLLEAQLNLGILYRMAGESARARSCFESFLAKASPAQYGEMIPKVREEL
                     ETLQ"
     gene            complement(7586..10993)
                     /locus_tag="VLE22_06015"
     CDS             complement(7586..10993)
                     /locus_tag="VLE22_06015"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB13994.1"
                     /translation="MERLQSQKFYLYAFCLLLLLPLVAVAQGDRATITGLVIDSTQAA
                     IPDVEITVTHVETNVQTSTKTGPTGVYNLLRLPVGTYTLVAKKDGFRTYQQTEIGARV
                     GETLRLDITMQLGQVTEQVTVTGAAPLIQSESAEVGMVLTSSTFTELPLTLGGGIRNP
                     SSFIFLQPGVTPGATWEKHVNGNAAFTDQVYYDGIALSRGDLANDAEVNPSVDAIAEF
                     KLIANNYSAEYTHALGGVTTFNYKSGTNDLHGNAFWLNSVEAYNARNFFQPSKPKYRQ
                     NNWGFTVGGPILLPKVYDGRNKSFFFFSFDQYYQRSPGSPGLGTTPTAKMLEGDFSEW
                     VDAGRGLVYNPRSQRTVDGKVVRDPFENNTVPKTMWSSISSKMVAMHPQPTYPGLANN
                     YQLDTGYPSTDQRSSGFKIDHLFTEAHRVSGMFNFTDRPAVKCPGANNVGGNGLAGDL
                     ECHNIQRVTTRIIRLNYDWTVNPTVLNRFSAGLSRFRNPNFSVHYGKGWPEKLGLKGV
                     GGDLFPWVDFNHDYIRFGDTIASDNYFTNFTFLDTLSVIRSNHTIRIGAEVQRARNNF
                     RDFGSSGGNWQFNQLSTGLPGVSKSGNAWASFLLGDVYSASSFFPFLQYGNRNGYYSL
                     WANDDWKVTPKLTLNLGMRWEVQPGFSDPNNRISYMDPTAPNPALGGIPGAYIFASDG
                     AFSSTGNTHWKDFAPRFGFAYRITDKTVVRGGYGIFFAQIITQGTGINGLREGYNISA
                     SFGSADLGVTPAMNWDNGFRQDFAHPPQLIPGLKNAQGATLVDRNRSTIVPYTQQFNF
                     LIEQQVGQNMSFSVGYVGNLGRRLHTGIDWNQVDPKYLSLGALLSKNILDPAVVAAGY
                     TEPFAGFASLWGARGTLAQSLRRWPQFTGVGQAGATFASSSYHSLQISGERRMAKGFM
                     FTLAYTFSKAMSDSEVFSSGAGRMDNFNRALDKSITSIDQPHILTVSYIYELPFGPGQ
                     KYLANGIASKVLGGWKITGLQMYASGLPISVSMNNNLPIGNAGQRPNVVSSDIRSGVN
                     SGAFEPARDVWLNASAFATPAPYTFGNAPRYTWARNMSTRNESLGLVKDTKWGERFNW
                     QFRFESSNPFNRVVWGGPVSNFSSGSFGRISSAGGSRQITLGTKFYF"
     gene            complement(11220..13280)
                     /locus_tag="VLE22_06020"
     CDS             complement(11220..13280)
                     /locus_tag="VLE22_06020"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HSB13995.1"
                     /translation="MKELALFLCVAAGLPAATPSLEALDAPGGGPIRLEVAGGERVAA
                     ISNYWRIEFDLRNGGVLDSIVFPAGSGKNLLLSPFASYVDQWSDENAPVTGFTQAQKG
                     SVVRLEFSGRMAAAGRRPGPIEYRTTWTISPFFIRADHTLRLSEDMTASAVGIGSTAV
                     RADLNEFGVRSGPMVDDVAWQQRINTRYGTIGSAGTKLVDDRHTLLYLLLFDRGVEGF
                     DLSTASDLATWERALAGRAGAGRFEASVSDDGSRIRVVCEPLRSERPVRLKKGEYMFS
                     YYLGLPRIVDKSNRKWRHLSFGNHPWPSDSEIARWAESGVNIVRLHNDYSEDENFWHD
                     GAWPPYDKKGMAEMRRVIAACRRHGIKVVPYFSIHELHPKAQGFTEHSPEWARTVDEL
                     GTVIHTLVGKGEYGALMCPTSGWLERRKHDIEQAYRELGFDGIYYDQVVNIGCTNTKH
                     GTNLHLGVDGVVELLAWTRRLVAPDGPLILHIGGNRPAIIFENFADLVVNMEELSGAE
                     SSLRLEDLPLLTLLAESLPRSPCPSYRRDRPLERNRRNISILTVFGMFPWAGSGRSEV
                     YEETLKLFRAFKPYRLETFKLHNALTGVVKTSRESVYGALYSSPEQTIVIVSNTRPEP
                     RKNVVWRVKAEDLGMPPAAKLTVKDVKTGQTQQVAPAALADGSFMTDLDGYEYRLFEV
                     SPAR"
     gene            complement(13287..15347)
                     /locus_tag="VLE22_06025"
     CDS             complement(13287..15347)
                     /locus_tag="VLE22_06025"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HSB13996.1"
                     /translation="MTSRLRGLCTAALFCTVSFAQAPQIQAPGGGSIQLDTSPGDRVK
                     VTTDYWHLEFDLRNGGALDTIVFKHGSGNNLLLAPFRTYVDRWCAANAPKTDFRPSRN
                     GDIVTLEFSGRMAAAGRNPGPVEFVTTWTLSPFVARADYKLRFLEDAMVSKAGVVSTS
                     LRRELNEWGLRVGMTDDPDRRKMAGGRFGKVQQAGEVLIAEHHAPTYMLFFDRAKEGF
                     DITVASDVATWETGLAKRGGVGRYEAKVADDGGSISLAMEPLSLPYPVKVAKGEYTFS
                     YYLGLPRIVEKANRKWMHLSFGNHPWPSDTEVKKWADNGVNIVRLHNDYAEDENFWHD
                     GAWPPYDDKGMAEMKRVIAACHRHNIQVVPYFSIHEFHPKAEGYAANENEWKRSVDQA
                     GTVFHNAWGKGEFGAQMCPQSRWLERRKQDIERAYRDLGFDGIYYDWVMTLPCTNKAH
                     NEKLHLGTDGVIDLLAWTRRLVAPNGTLILHLYGAMPSIAFENFADLVVNMEEISGAE
                     KLMVMDEVPIVTVLAESIMRSPCPSYRRDMPVERNQRNIAHLVVLGMFPWSGGTGDGV
                     YAETLKLFRTFKPYRLEDYRFHDALTGVVKTSWETVYGALYSSPEQAVVIVSNARPEK
                     RKNVVWRVEAEDLGMSPAAKLTVKDVKTGQTQQVAPAALSDGSFMTELDGYEYRLFEI
                     APAK"
     gene            15438..18320
                     /locus_tag="VLE22_06030"
     CDS             15438..18320
                     /locus_tag="VLE22_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB13997.1"
                     /translation="MKAYAWVAVCLASALFLSPASSQQRKPVRVYSTLLNPREHPDDG
                     RRHVRPPSWETFGHRTLFTALRGFEVKDGRLVRYKEELDKYTREHELAEVIWPSYPIL
                     FANNLGDLADEIRDRKLFLFDIWGYVPGSGPGGYWQQFEPPPGVFQMLESKLGDRWLG
                     MDVGEQDGRYIGGYAPQMYPVSAGRFDQYLNFHRHFERFTSDLGNRMATLVSLNFGHY
                     FLKEGIYTLIGAETAQALPNGQVYYSFIRGAGKQYGVPWFGNASVWNRWGHKTYSDSG
                     SDFGPTKGTSVSLLKRLLYSHILYNSLLVGFESAWFDGGGKLTPIGHVQESANRWVKQ
                     NGQPGVMMTPIAVMADFFSGWSFPRHLYSRHVYRVWGNLPYESGDHLTDGVLDMLYPG
                     YQDSSYFHDERGFITQTPYGDAADALLSDAPGWLLARYPVVVVAGELSGDIETRDKLE
                     EYVRQGGHLVITAGNIAKWNGGIGGVTAAGPARSFAPKQALQVAGSRVVEDRAFEAIG
                     LRLQAVHKVLVRSTGTLAAEVPLGRGRLTALGSLFGVPKEAVIQTVRNEIDKPLAKPY
                     PLLKHVRMILDRVFREQVLFDAGEGLSLIVCRKEAGEYTLGITNNSWRERPFHIVSHI
                     GKIESVNELPLDRSEWGATGHVPEGVDASALGSSTNRTIAGGDIRVLRVRVRDENVEE
                     IRHAAPPPGPRGRALPLRGDGSIQEQILRRPSFFQHFDTAVIDWRYLNSRETEALNKE
                     ARWLKLQGLKVIVDLTSGLNLYPDLRIIDNIGSDYAASMRAIDSVLSKMQILDSRDLI
                     LSLHRVPENNFTRDETWAGFEKTLRRICGQAESARITVHLRMAPGKPPSDLKEAAAFV
                     ARVNMPNLKIAPSTAMLLAAKNGPEALSAVPPEKVGMWLVATPASDIGGSVWQYHGPV
                     AGKDLEALVRPFLAAAPGVPLVLDAVYPDQDAEYRDARALDRLAGK"
     gene            complement(18399..19643)
                     /locus_tag="VLE22_06035"
     CDS             complement(18399..19643)
                     /locus_tag="VLE22_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB13998.1"
                     /translation="MIRTISTVIVCLFLLAAAAPGQQQFHNPERVAAKTFPIMAWGGS
                     PSDQAQLNLMKDAGLNASGFCRVEDVGKVQDAGLSCFVSDKRVNGYDWANMPSESEIR
                     RNVAEVVKATASNPAVLGYYLRDEPHASLMPAMGRVSKMLKEAAPDKWPYVNLFPYRV
                     SKERMGTPDYDTYAKMLVDTIGQPFLSYDNYSVVGGEMLEYFYNNLEAVRRISQETNT
                     PFWNCILANAHFNYMEPSDATFNLQVYATLAYGGRGIQYFTYYSPHNGNYRLGAVDQF
                     GNKTATWDALRRINLQIHALAPTMLKLKSTGVYHYPDVPEFGKPLSQSKLIRSVPMVM
                     RYVKPPIQGRFLIGEFEDGQGRPYFMIVNKDLNNSFQFSIELKEQGKKLIRICNYAGI
                     EEEFGREMDWLAPGAGILFRVE"
     gene            complement(19665..22916)
                     /locus_tag="VLE22_06040"
     CDS             complement(19665..22916)
                     /locus_tag="VLE22_06040"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HSB13999.1"
                     /translation="MSRIFLTFALTLYLLATGAHAFGQVKAWEEKLTMPTWEIGPPQK
                     HPVFLDGGRDIYPYPLQETLTNNKVDKAYTAVYLENEYIKVLVLPEIGGRLHGALDKT
                     NGFEFFYWQKTVKPGLISMTGAWISGGIEWNFPHGHRPSCFMPTDHRIVKNADGSATV
                     WVGETEPIFRMRWLVGMTVYPGRSYIKADYVFINPTQHKHSFQFWATAATYAGDPVQA
                     QYPGDMVTGHSKHEFWNWPVHNGVDLTWWKNVPNASSFFAFNNPSEWFGTYDHQKQAG
                     MVHVGNHHIVLGKKLWTWGSGPSGRIWDDILSDGSGPYYEPQAGVWSDNQPDYHWMGA
                     YEVKTAHDYWYPVRDLRGYRNASKDFAVNTDLKDGKAFGGVNATGVFSNCKVVLKDTK
                     TGKVLSEAAATISPDKPYTVEVPAGGATVYDLHLAVYDAKGKLAIELQQKPPQKVDLP
                     PGQTDPGDPKKMNPDELYHAGEWLDRFRRTNEALVYYNEALSRDPKDSRVNAEMGFLA
                     LKQGKWNEALTYLNTALERDSDNARLYYGKGLALSGLRKFKEAYDQFYRAIYGYEYFV
                     PAYLNLAKIDMLDGNYREAIAKLDEAGKQNDKFADIPALKAAAYRHLGDHKRALTAAE
                     KALDLDAMNFMGGYEKTLAVKNTGKAADPWGSIWSGYMRDAVQNYLELAAAYAGAGQF
                     VDADDVLARYAEGKDVTRISPLVSYFRGYFKELTGDKTTAAQFYAVARKGSPINVFPH
                     RLEEKAALEAAIRAYPDDANARLFLGNLLYGSGQREEGFADWQKAVQLNPQSPLAQRN
                     VAYGQRYLKKDMQASYEAYQKAFAVDATDGRILLELDDVAQQIKVSEAERYALFMKHP
                     KTVNSRDDLISTLIDLRLKQGGTENLKAVYDVLKNHHFHSWEGKYEIHYAWVEVNKQL
                     GDAAFDRKDYQTALAHYQQAGEYPKNLEVAARTPDFQAHVNWDLARVYQAMGRRDTAQ
                     QHLKKILDEKYGKAHLGMYYQMLANKTLGQTGEYKALLGKLEAEARERTSGKYEYRGL
                     PETIGYYLLSLVLEEKGDTQGAAAARQKAIGMDAMVARLSLREAQIEFAGAHQ"
     gene            22992..23708
                     /gene="mtgA"
                     /locus_tag="VLE22_06045"
     CDS             22992..23708
                     /gene="mtgA"
                     /locus_tag="VLE22_06045"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HSB14000.1"
                     /translation="MKSALRRRPRKTQSGTKSRLLRRIARWLSIGLVAFIGLCGLSLL
                     LMRWIDPPFTAVHAERRIESLFRKGRYQKRYRFVPLRRISVPLQHAVIAAEDGRFYQH
                     WGLDVLEMKKVVEDDWTRGRMRGGSTITQQLVKNLFLTTRGSVIRKGFEFVLAPLAEV
                     LLPKDRILELYLNVIEWGPGVYGAEAAARQYYATSAVAIGREQAARLAAIIPSPLRRR
                     PGRMDDYSSEILERMSRMGW"
     gene            complement(23757..>24953)
                     /locus_tag="VLE22_06050"
     CDS             complement(23757..>24953)
                     /locus_tag="VLE22_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB14001.1"
                     /translation="AATPRIYVIGHAIRAPKPEELEKMRQLVREAMREGALGIASSLI
                     YPPGNFAKTEELIELAKVAAESGGIYISHLRSEGSQLLPSVDELLRIAREAKIRAEIY
                     HLKASGKANWPKMTEAIAKVDQARAQGLHVTADIYTYPASSTGLDSVMPPWTQEGGYE
                     AFLKRLKDPATRKRIVQEMKRESDQWENMYLGAASPENILLVDFRKAELKPLTGKTLG
                     EVARMRKKSPEETAMDLILEDESRIGTVYFTQSEDNVRKAIATPWVSFCSDSPSLAPE
                     GVFLKSNVHPRAYGSFARLLGKYVRDEKIIPLEEAVRKLSALPAENLRLNRRGMLKAD
                     YYADVVVFDPATIQDHATFEKPHQYATGVQYVFVNGVAVLWEGDHTGAKPGRFVKGPG
                     YSGAER"
BASE COUNT         4509 a         7577 c         7679 g         5188 t
ORIGIN      
        1 caatgtttcc tcatagaacg gggacgcggt gacgaaaatg cgctcgtaca ggttattgcg
       61 ttcgccgttc gttttcgcga tatagcgcat gccgccgttg atctcggcgc cgtccgcggt
      121 ggctttggga tccttcgcat agaacggctc cgacgcgttg gtgcggtacc agtcaaacca
      181 gatcgaggtg aacatcggct tgtccgcgtt tccggacatc agcacacgag ggttggtctg
      241 cccgtaggtg atgtagggca cgttaatcag cttcggattc gctactccgg ctacgcgccc
      301 gaaccggaac tccgtcgcct ggcctccgtc gcaccacacg tcgagaacaa tcgacttctg
      361 ccagaggcgc aggttgtagt cgacaatctg ggaaccgtga cggaacttcg cctcgatcac
      421 gtcgccattg acggccgcgg agacgagccg cccttccgat atcttccctt cgggcgtgcc
      481 ggcgaaccgg atgccacccc cgtccatggg ccggaacgag gcgccgccgt tgacgctcgc
      541 cgttaattcg ctcatgttgc cggcgcttgg acggtactcg tacctcacag tgccgtcctt
      601 gcccttgtac tgaagctcga aacggttctt ttcgacctgc cgcatcgtga cctggaattc
      661 tttctcgaag ttcagcggga ggatggtctc ttcacgagtt gggaagggta gcgtgccctc
      721 gccgatgttc atgccggcga tctgtccacg gaagggtttc agattgcgct tcggttgcgg
      781 ctgaagggtg agcggcttca gttcctcggt ttcgaaggcg agcgagtcgc agaagaagta
      841 gcgcaggtcg gtattcttgc ccttggaaat ctcgatcccg gtgaaagtgg ccggccagac
      901 gatctgcttc agcgtcgcgg cggggacttt ccggtgaatc agccaccact gcttccactg
      961 gatgttggtg agctggatgg tgtgctcctt gccgcgagag tcggtgacga ggatggagac
     1021 atcggccgcc ggcgtcgtcg ggtcaggaac ccacgaccag cggttgccgt agccccacaa
     1081 ctcgatcgag tcgaacttgt cgggtattgg aattggcttc ggcggccgcg cgacgacgcg
     1141 actcttctcg ctcgtcccgg aatacaggaa cttcgcaacg tactggcccc acatttgctg
     1201 ttcgcggctg cggcgcagtt cgccctttgc gccgtcgaac atctcgagct tccatccgtt
     1261 gaggtcctcg aagtcaacga gcgtcttcgg gtgttcggcg cgctggaccc aggtgaattc
     1321 gtaaggctgc tggcccacct gttgccatag ctcgatgtcg ttgatcgccg gcgcgacttt
     1381 gtccggggat tgggccagcg ccggaagaat cgagtagaga acgacaacgg ctgaaatgat
     1441 ggctcgcata agtctccttg ggtatttgag aaatggggac agacagccat tttgcaacta
     1501 cggcaaaatg gtggtcagtc cccattcctc agattccaaa tcatataaga aagccctccc
     1561 ggccggtgaa gccgagaggg cggaaggtta aacggttact agcaacgaag cctagaagta
     1621 gaacttcgtg cccaatgtga gcgaccggga gccgcgggcg ctggtgatct tcccaaacga
     1681 gcccgatgag aagttgctca ccggtccgcc aaacaccacc cggttgagcg ggttcgagga
     1741 ctcgacgcgt aactgccagt tgaaccgctc accccacttc gtgtccttca acaggccgag
     1801 ggattcctgc atgaaggccg ggctgcgcgc ccaggtgttc cgtggcgcgt tgccgaacgt
     1861 gtacgccgcc gggatcgcaa acgccccggg gttcagccaa aggtccttgg ccgggtcgaa
     1921 agcattcgcg ctgacgctgc tccgcagatt gctcgaaaca atgtccggcc gctgcgacgc
     1981 gttgccgatc ggcaggttgt tgttgatgtt gaacccaagc ggcgtgccgc tggcgtatat
     2041 ctgcatgccg gtgatcttcc aaccgcccag gatcttcgac gtgacgccct gcgccaggta
     2101 cttctgtccc ggcccgaatg gcagctcata aatgtagctc aagctcagcg tgtgcggctg
     2161 gtcgagggag gcgagggact tgtccaactt ccggttgaag tagtccatcg ttcccgcgcc
     2221 gtagccgtat acttcggtgt cgttcatcga tttggagaag gtgtaggcca aagtaaacat
     2281 caagcccttg ctcatccgcc gcaggccgct gatctgaagc gagtggtaat tcgaagagcc
     2341 gaaaaaggcg cctgcctggc cgacgctcgt gaattgcggc cagcgtttca gcgcctgtgc
     2401 gagcgtcccg cgggaacccc atagcgtcgc gaatcccgca aacggctcct ggaacccggc
     2461 cgcgacgacc gccggatcga gaatgttctt tgaaagcaga gagccgagac tgagatactt
     2521 cgtctcattc tggttccagt tgatgccttt cgatagccgc cggcccccgt tccctacata
     2581 gccgatggtg aaagacatgc tctggcctac ctgccgttca atgatgaagt tgagttggtg
     2641 cgtgtaggag acgatcgtgc tgcggtctcg atccaccaac gttgcgctct gggtgttctt
     2701 caagcccgga atgagctggg gcggatgagc gaagtcctgc cggaagccgt tgtcccagtt
     2761 catcgccggt gttacgccaa ggtcgggcga actgaaactg gcgctgacgt tgtaaccttc
     2821 gcgcaaaccg ttcacgccgg tgccctgggt aatgacctgg gcgtagaata tgccgaagcc
     2881 gccgcgaatc accgtcttgt ccgtcacgcg gtaggcgaag ccgaatcgag gagcgaagtc
     2941 cttccagtgc gtgttgccgg tgcttgaaaa ggccccatcg ctcgcgtaga cgtacgcgcc
     3001 tgggatgccg ccgagcggtg tgttaggcgc cgtcggatcc atgtaggaga ggcggttgtt
     3061 cggatcggtg aagccgggct gtatctccca ccgaaggccg agattcaagg tcagcttcgg
     3121 cgtaaccttc cagtcgtcgt tcacccacgg gctgtagtag ccgttgcggt tgccgtactg
     3181 aaggaacggg aagaatgccg aggcggagta gacgtctccc agcaggaagc tggcaaaggc
     3241 gttgccgctc ttggagacac cgggaaggcc ggtcgagagc tggttgaact gccagttgcc
     3301 gccggtcgta ccaaagtccc gaaaattgtt ccggtgccgc tgcacctcca cgccgaattt
     3361 cagcgtgtgg ttgctgcgca tgacactgag cgtgtcgagg aaggtgaagt tggtaaagta
     3421 gttgtccgaa gcgatggtgt ccccgtaccc ggcgtagtcg tggttgaagt ccacccacgg
     3481 gaacaggtcg ccgccgaggc ccgtcagtcc cagtttctcc ggccaaccct ttccgtagtg
     3541 caccgagaag ttgggattgc ggaagcgcga caggccggcc gagaagcggt tcagcaccgt
     3601 cgggctgacc atccagtcat agttgagccg gacgattcgc gtcgtcaccc gctggatgtt
     3661 gtggcactcc agatcgcccg ccaggctggc agcgcccgag ttgttgtggt tcggacactt
     3721 caccgccgga cggtcggtga agttgaacat gccgctgacg cggtgcgctt cggtgaacag
     3781 gtggtcgatc ttgaagccgc tcgaccgctg atccgtgctc ggagcgccgg tctcgagctg
     3841 atagttactg gtcaggcctg ggtacgtggg ctgcgggtgc atccctacca tctgcgtgga
     3901 aatgcggctc cacatcgact tcggaacgac gttgttctcg aacggatcgc ggatcacttt
     3961 gccgtccacc gtgcgctgcg aactcgggtt gtagaccaag cccctgccgg agtccaccca
     4021 ctcactgaag tctccttcga gcatcttcgc ggtcggcacg gtcccgaggc ccggcgagcc
     4081 gggggcgcgc aggtagaact ggtcgaacga gaagaagaag aacgacttat tgcggccgtc
     4141 atagatcttc ggtagcagga tcggaccgcc caccgtgaag ccccagttgt tctgccggta
     4201 ctttggcttg gagcgctgga agaagttgcg ggcattgtac gcctcgacgc tgttcagcca
     4261 gaaagcgttg ccgtgtagat cgttggtgcc gctcttcatg ttgaaggtgg tgacgccgcc
     4321 cagcgcgtgc gtgtattcgg ccgtgtagtt gttggcgatc agcttgaact cggcgatggc
     4381 gtcgaccgac gggttcacct cggcgtcatt ggccaggtcg ccgcggctga gcgagatacc
     4441 ctcgtaatag acctggtcgg agaaggacga gacgccgttg atctgcttcg tccacgtgga
     4501 gcccggtgtc acgcctggct ggaggtagat gaaggaggac gggttgcgga ttccgccgcc
     4561 gagcgtgagc ggcagttccg tgaactgcga cgaggtaatg gtcatgccga cctgggcgtt
     4621 ctccgattgc accagaggcg ccgcgcccgt gacgttcacc gtttcggtca cctggccgag
     4681 ttgcatggtg atgtcaaggc gaagcgtgtc accctccagc accgggatct gggtctgctg
     4741 ataggcccgg aacccgtcct tcttggctac gagggtgtag gtgccgatcg gcacgcgcat
     4801 cagtgtgtaa acgccggtcg gtccactgcg cgctgtggtg gcaacgttgg tcgcagtgtg
     4861 cgttatggtg atttcaacgt ccgggatcac tgcctgggta ctgtccgtca ccaatcccgt
     4921 gatgttgcct tgggcaaatc ccagaattgg cagcagaagc agcaacgaca ggactgacaa
     4981 gacccggaga tataaattct ctgaagacaa tcttgtcatc ttgacctctt tcacgaactc
     5041 agcccgttgg gtgaagctat agacttcacc tgatcgtgcc ggaactcctg gtacgagcta
     5101 aaaacctctc cccgtagcac tctagactag gagcgtcccg aagtcaagaa aaaaatcaga
     5161 gacctagacc cgattctcgc catgacggaa gcaacgttac gggcgctcga cggccgaccc
     5221 aataaagctt ctttcattag aggttttgtg cgtttggctc gcgcaaccac cctaaccgcg
     5281 gcttggcgcg tcagggcagc gtcgagcgag cgaggaccgg gagctgggca gcgcgtctga
     5341 ctattgcagc gtttccagtt cttcacgaac cttgggtatc atctcgccgt actgcgccgg
     5401 cgaagccttc gccagaaagg actcgaagca acttcgtgct cgcgcgctct cgccggccat
     5461 cctgtatagg attccgagat tcagttgcgc ttccaacagg tcgggatcca gttgcacggt
     5521 cttttcgaag tagctgcgcg cagccgccgt gtcctgtcgc tgaatggcga tcacgcccaa
     5581 gccgttgtac gctgccgcgt gcgtttcgtc gatcttaatg atgcctttga agatacgctc
     5641 cgcatcctcc aacctgccgg tctcgacgta agcggtagcc agattcgttt gcgttcccgt
     5701 atccgcgggg tccaaccgga tggccctctc atactcgggg atggcctccg tctttcttcc
     5761 ggagcgtacg agaaaatctc cgatacggga acgcagccag ccactctcga cgccgttccg
     5821 gatcgccgta cggtatacgt ctatcgcctc ggcgtagcgg ccggctcttt cgcaattgcg
     5881 tcccagcgaa tagtagagag tcggattcgc cgcatcctgt ttcagagcct gcctcagaag
     5941 ttcgatccgg tgggggaagg gaagatgcga tgtcggcccg tcggcttctt ctatggcttt
     6001 taggacgccg agacgatctt tcgggtcgac tcccttgccc ttcaggtcaa acgtcggctg
     6061 agaaactcct gaaacgtagc cgagtgatct caactgctcc agagtccgtt gatcgaccgt
     6121 ggacgtctcg accttttccg ttcccttgcc ggcgatggct gccaattccg cttggagttt
     6181 ctgcgcttca gcggcgtggc tctgaatcac gttgttggtt tccgctggat cctgcgaaag
     6241 atcgtagagt tccggtttgg gcgcaaggat atatttccac cggttcgtac ggatgccgcg
     6301 cagttccgcc cagcccatgt tgattttcgg atacagcgtc tctgcgtagg agacgtctgt
     6361 ggcagcgttc tttcccgcga agaaaggcgt caggctgacg ccttggaccg aagcaggcgg
     6421 acggccgccc atcaattcca ggaccgtcgg caggaggtcc acggttcgcg cctgctgttt
     6481 cacccgcagg ccggccggaa tcccgggtcc ggacataatg aacgcgatcc gcaaggtgga
     6541 gtcgtacaca aatacaccgt gcgagtactc cccgtggtcg ccaaggctct caccgtgatc
     6601 cgatagcacc gcgattagcg tcttgccggg cggggatttc ttcgagaccg catcgaacag
     6661 gcgtccgagt tcgtggtcgg cgtatgcgat ctcgccgtca taagggcggt ctctatactg
     6721 attccggaat ggagccggcg gattgtacgg cacatgggga tcgaagacat gcacccagag
     6781 aaaaaacggt ttgccggatt gtgcgtccat ccagcggagt gctcggtcaa ccgtctgccc
     6841 cgcgggccgc tccgcatccg cgacgaattc ttgcccttta ccggccttgg gcatctgatc
     6901 gtcgtaaacc gcgaagccct ggttcaagcc gaataccctc tttagactta cggaactgat
     6961 gaatgcagcc gtatcccagc cctgctgttg gaggatcgta gctagggttg tagtggaggg
     7021 cctcaagatg aatccgccat tgccgcgcac gtgatgagct gtaggataca ggccggtgaa
     7081 catactggcg tgcgaaggag gcgtcaacgg cgtttgggta acggcattct cgaacaggac
     7141 gccgtcttgt gcgatgcggt cgacgctcgc ggtttcgatt tttggatagc cgtaacagcg
     7201 caggtgatca gcgcgcaatg tatcgatcgt gaccacgaca aggttgaggg gacgtagggg
     7261 tgtcgtggag gccgtcctcg cgccgctgct ctcagagtgt ttgcaggagc tgagaatggc
     7321 tgttgcaagc agacacgcac acgcgagtgc attcggcagc cgggggaagt aggacctttt
     7381 ggattgcact ggaagagcca cctagcaact ctaggtcacg atagcagatc acggcgcaac
     7441 cgttcagccc ccctccaccc ctccagcagc gacagcgcgg gctgaccggt ccggcgccgc
     7501 ctgtaatgaa agcccgacaa taaaaagccc tcccggccgg tgaggccgag agggctgaag
     7561 gttcatagga gttcggccgc gaagtctaga agtagaactt cgtgccgagt gtgatctggc
     7621 ggctgccgcc ggcgctgctg atgcgaccga acgaaccgga cgagaagttg ctcaccggtc
     7681 cgccccagac cacacggttg aacgggttgg acgactcgaa gcggaactgc cagttaaacc
     7741 gctcgcccca cttcgtgtcc ttcacaaggc cgagcgactc gttccgcgtc gacatgttgc
     7801 gcgcccatgt gtaccgaggc gcattgccga acgtgtaagg cgccggtgta gcgaaagcgg
     7861 aggcgttcag ccacacgtct ctggccggct cgaacgcacc cgaattgaca ccactgcgga
     7921 tgtcgctcga aaccacattc ggccgctggc cggcgttgcc gatcggcagg ttgttgttca
     7981 tgctgaccga aatcggcaag ccgctggcgt acatctggag gccagtgatc ttccagcccc
     8041 ccaaaacctt tgaggcgatg ccgttggcta ggtacttttg gcccgggccg aacggcaatt
     8101 cgtagatgta gcttacggtc aaaatgtgcg gttggtcgat cgatgtgatg gacttgtcta
     8161 acgcgcggtt gaaattgtcc atccgtccgg caccggagga gaaaacctcg gaatcggaca
     8221 tcgctttgga gaacgtgtag gccagagtga acatgaagcc cttggccatc cgccgctcgc
     8281 cgctgatctg gagcgagtgg tacgaggaag aggcgaacgt cgcgcccgct tggccgacgc
     8341 cggtgaattg cggccagcgc cgcagtgact gcgccagcgt tccgcgagcg ccccacaacg
     8401 aggcgaaacc tgcgaacggc tccgtgtagc cggccgcgac gaccgccggg tcgaggatat
     8461 tcttcgacag cagcgcgccg aggctcaggt acttcggatc tacctggttc cagtcgatgc
     8521 cggtgtgcag ccgccggccg aggttgccca cgtacccgac gctgaacgac atgttctggc
     8581 ccacctgctg ctcgatcagg aagttgaatt gctgcgtgta gggaactatc gtgctgcggt
     8641 tccgatccac gagcgtagcg ccctgggcat tcttcaggcc gggaatgagc tggggcggat
     8701 gggcgaagtc ctgccggaag ccgttatccc agttcatcgc cggtgtgacg ccgaggtcgg
     8761 ccgagccgaa actcgcgctg atattgtagc cttcgcgcaa gccgttgatg cccgtgccct
     8821 gggtgatgat ctgggcgaag aagatgccgt agccgccgcg gaccaccgtt ttgtccgtga
     8881 tgcggtaggc gaagccgaag cgcggcgcga agtccttcca gtgtgtgttg ccggtgctgc
     8941 tgaaggcccc gtcgctcgca aaaatgtacg ctcccgggat gccgccaagc gccgggttgg
     9001 gcgccgtagg atccatatag gagatgcggt tgttcgggtc gctgaaaccg ggctgcactt
     9061 cccagcgcat gccgaggttc aaggtcagct tcggagtgac cttccagtca tcgtttgccc
     9121 acaggctata gtagccgttg cggttgccgt actggaggaa cgggaagaac gacgacgcgg
     9181 agtagacatc acccagcagg aagctggccc aggcgttgcc gctcttggaa acgcccggca
     9241 atccggtcga gagctggttg aactgccagt tgccaccgct cgatccaaag tcccggaagt
     9301 tgttccgggc gcgctgaacc tccgcgccga ttctgatcgt gtggttgctg cggatcacac
     9361 tcagcgtatc gaggaacgta aagttggtga agtagttgtc cgaagcgatc gtgtcgccga
     9421 agcggatgta gtcgtggttg aagtccaccc acggaaacag gtcgccgccg acgcccttca
     9481 gccccagctt ctccggccac cctttgccat agtgcactga gaagttcggg ttgcggaaac
     9541 gcgacaggcc ggccgaaaac cggttcaaca ctgtcgggtt caccgtccag tcataattca
     9601 gacggatgat gcgagtggtc acgcgctgga tgttgtggca ctccaggtcg ccggccaagc
     9661 cgttgccgcc gacgttgttg gcgccagggc acttcaccgc cgggcggtcg gtgaagttga
     9721 acatgccgct gacgcggtgc gcttcggtga acaggtggtc gatcttgaaa ccgctcgatc
     9781 gctgatccgt gcttggatag cccgtatcca actggtagtt gttggccagt ccaggataag
     9841 tcggctgcgg gtgcatcgcc accatcttgc tggagatgct gctccacata gtcttcggca
     9901 cagtattgtt ctcgaacgga tcgcgaacca ccttgccatc caccgtgcgc tgtgaacgcg
     9961 ggttgtagac caagcccctg ccggcgtcca cccactcgct gaagtcgcct tcgagcatct
    10021 tcgcggtcgg ggtggttccc aagccgggcg agccggggct ccgttgatag tactggtcga
    10081 acgagaagaa gaagaacgac ttgttgcgac cgtcgtagac cttcggcaac aggatcgggc
    10141 cgcctaccgt gaagccccag ttgttctgcc ggtacttggg cttagacggc tggaagaagt
    10201 tgcgggcgtt gtaggcctcc acgctgttca gccagaaggc gttgccgtgc agatcgttgg
    10261 tgccgctctt gtagttgaac gtggtgacgc cgcccaaggc gtgggtgtac tcggccgagt
    10321 agttgttggc gatcagcttg aactcggcga tggcgtcgac cgaggggttc acctcggcgt
    10381 cgttggccag gtcgccgcgg ctgagtgcga tgccgtcgta atacacctga tcggtgaagg
    10441 ctgcgttgcc gttgacgtgc ttctcccacg tagcgccggg ggtgacaccg ggctgtagga
    10501 agatgaatga ggacgggttg cggattccgc ctccgagtgt cagcggcagt tcagtgaacg
    10561 tggacgaagt gagtaccatg ccgacttcgg ccgattcgga ttgaatcagc ggtgcggccc
    10621 cggtgacggt gacctgttcg gtcacctggc cgagttgcat ggtgatgtcg aggcggagcg
    10681 tttcgccaac gcgtgctccg atttccgtct gctgataagt ccggaagccg tccttcttgg
    10741 ctaccagggt ataggtgccg acaggcaacc ggagaaggtt gtagacgccc gtgggaccgg
    10801 ttttggtgga tgtctggacg ttggtttcaa cgtgcgtgac ggtgatttca acgtccggga
    10861 tagcggcctg cgtgctgtct attaccaatc ccgtgatcgt ggcgcgatcg ccttgcgcca
    10921 cggcgacgag aggtaacagg aggagcagac agaacgcgta cagatagaac ttctgcgact
    10981 gcaaacgttc catatttgac cccccatgtt cacggggtct tgtccctaca agctgccccg
    11041 cgagagaaaa tacatcctag caatgtcgat atattgtatt ttcgtagtaa ttctcttttc
    11101 aaggggaaaa gttcaaagtg tcaatagctt aatggtaagc gctgacattc gtttgtatgt
    11161 ttagtgttct taatgtgagg cccacggaca atcgaggttc cgcacaccac tcaaactgtt
    11221 tatctcgccg gcgaaacctc gaatagccgg tattcgtagc cgtcaaggtc ggtcatgaag
    11281 gacccgtccg cgagagccgc gggagcgacc tgctgcgttt ggccggtctt cacgtccttg
    11341 accgtgagct tcgccgccgg cggcattccg agatcctcgg ctttcacacg ccagacgacg
    11401 ttcttgcgcg gctcggggcg ggtgttggac acgatgacaa tggtctgctc cggcgaactg
    11461 tacaaggcgc cgtacactga ctccctcgac gttttcacga cccctgtcag agcgttgtgt
    11521 agtttgaacg tttcgagcct gtacggtttg aacgcccgaa atagcttgag ggtctcttcg
    11581 tagacctcgc ttcgcccgct tccggcccac gggaacatac caaacacggt caggatggag
    11641 atgttccgcc gattgcgctc gagcggccgg tctctccggt aggatggaca tggcgacctc
    11701 ggcaggctct ccgctagcag ggtgagcagc ggaagatcct cgagccgcag cgacgactcc
    11761 gcccccgaca gttcttccat gttcacaacc aggtcggcga agttttcgaa gatgatcgcg
    11821 ggcctgttgc cgccaatatg caggatcagc gggccgtccg gagcaaccag cctccgagtc
    11881 caggccagca actcgacaac gccgtcgact ccgagatgca gattggtccc gtgtttcgtg
    11941 ttggtgcagc cgatgttgac gacctgatcg tagtagatgc cgtcaaatcc gagttcccga
    12001 tacgcttgct cgatgtcgtg ttttctccgt tcgagccacc ccgacgtcgg acacatgagt
    12061 gccccgtact cgcccttgcc aacgagcgtg tggatcaccg tcccgagttc gtcgaccgtc
    12121 cgcgcccact cgggcgagtg ttctgtgaag ccctgcgctt tcggatgcag ttcgtgaatc
    12181 gagaagtacg ggactacctt aatgccgtga cgccggcaag ccgcaatcac ccgccgcatc
    12241 tcggccatgc cctttttgtc gtaaggcggc caggccccgt catgccagaa gttctcgtcc
    12301 tcggagtagt cgttgtgaag ccggacgatg ttgacgccgc tctccgccca ccgggcgatc
    12361 tcgctatccg acggccaggg atggttcccg aaggacagat ggcgccattt ccggttcgac
    12421 ttgtcaacga tgcgcggcaa gcccagatag tagctgaaca tgtactcgcc cttcttgagg
    12481 cgaacagggc gctccgaccg cagaggttcg cagaccacgc gaatccggga gccgtcatcg
    12541 gagacgcttg cctcgaaccg gccagcgccg gctcttccag ccagcgcacg ctcccaggtg
    12601 gccaggtccg aagcagtgct caagtcgaac ccctccacac ctcggtcgaa caacaaaagg
    12661 taaagcagcg tgtggcgatc atcaaccagt tttgtcccgg cgctcccgat cgttccatac
    12721 cgcgtgttga tccgctgctg ccacgcgacg tcgtcgacca taggcccgct ccggacgccg
    12781 aactcgttca ggtccgcgcg gacggctgtc gagccaatgc ccaccgccga cgccgtcatg
    12841 tcttcgctca gccgcagcgt gtggtccgcc cggatgaaga agggcgagat cgtccacgtc
    12901 gtccgatatt caatcggccc ggggcgcctt ccagccgcgg ccatcctgcc gctaaactcc
    12961 agtcggacca cgctgccctt ctgagcctgg gtgaagcccg taacgggagc gttttcgtcc
    13021 gaccactgat cgacgtagct cgcgaagggt gacagcagca gattcttccc ggacccggcc
    13081 gggaacacga tgctgtcgag cacgccgccg ttccgcaggt cgaactcgat ccgccagtag
    13141 ttgctgattg cggcaacgcg ctcgccgccg gcgacctcca atcggatggg acctcctccc
    13201 ggggcgtcga gcgcttccag ggaaggcgtg gcggcgggaa gcccggccgc cacgcacagg
    13261 aacagagcca gttctttcat cagtgtctac tttgccggcg cgatttcaaa cagccggtat
    13321 tcgtagccgt caagttcggt catgaaggac ccgtccgaga gagccgccgg ggcgacctgc
    13381 tgcgtttggc cggtcttcac gtccttgacc gtgagcttcg ccgccggaga catgccgaga
    13441 tcctcggctt ccacacgcca gacgacgttc ttgcgcttct ccggacgagc gttagacacg
    13501 atgacgacgg cctgttcggg cgagctgtat aaggcgccgt acaccgtctc ccaggaggtc
    13561 ttcacgaccc cggtcagcgc gtcatggaat cggtagtcct ccaggcgata cggcttgaac
    13621 gtgcggaaca gtttcagcgt ctccgcatag acgccgtcgc cggtaccgcc cgaccacggg
    13681 aacatgccta gcacgacgag atgcgcgata ttccgctggt tccgttccac cggcatgtca
    13741 cgccggtaag acgggcacgg cgagcgcatg atgctttccg ccagaacagt gacgatcgga
    13801 acctcgtcca tcaccatgag cttctccgcg ccggagatct cttccatgtt gaccaccagg
    13861 tcggcgaagt tctcgaaggc gatggacggc attgcaccgt aaagatgcag gatcagtgtg
    13921 ccattcggcg cgacgagtct ccgcgtccag gccagcaggt cgatcacacc gtcagtgccg
    13981 agatgcagct tctcgttatg ggccttgttg gtacacggga gcgtcatcac ccagtcgtag
    14041 tagattccgt cgaaacccag gtcccgatac gccctctcaa tatcctgctt gcggcgttcg
    14101 agccagcggg actgcgggca catctgcgct ccgaactctc ctttgcccca ggcgttgtgg
    14161 aagacggtgc cggcctggtc gacgctgcgc ttccactcat tctcgttcgc cgcgtagcct
    14221 tccgctttcg gatggaactc gtgaatcgag aagtacggga ctacctggat gttgtgccgg
    14281 tggcaagccg cgatgacacg cttcatctcg gccatgcctt tgtcgtcata gggaggccag
    14341 gcgccatcgt gccagaagtt ctcgtcttcg gcgtagtcat tgtgcagccg gacgatgttc
    14401 acaccgttgt cggcccactt cttgacctcc gtgtccgacg gccaggggtg gttgccgaac
    14461 gaaagatgca tccacttccg gttggccttc tcgacgatgc gcggcagccc gaggtagtag
    14521 ctgaacgtgt actcgccttt ggcgaccttc accgggtacg gaagactcag cggctccatg
    14581 gcgagggaga tcgagccacc gtcgtcggcg accttggcct cgtagcggcc cacgccgcct
    14641 cgcttcgcca ggcctgtctc ccaggtagcc acgtcggagg cgacggtaat gtcgaagccc
    14701 tccttggctc ggtcgaagaa caacatatac gtaggcgcgt ggtgctcggc gatgagtact
    14761 tccccggctt gctggacttt cccgaaacgc cccccagcca tcttccgccg gtcgggatcg
    14821 tcggtcatgc cgacgcgcag tccccattcg ttgagctcgc ggcgcagcga ggtggaaacg
    14881 acgccggcct tagagaccat ggcgtcttcc aggaaacgca gcttgtagtc ggcgcgcgcg
    14941 acgaacggcg acagagtcca ggtggtcacg aactcgaccg gccccggatt tcttccggcc
    15001 gccgccatcc ggcctgagaa ttccagcgtg acgatgtcac cgttccgcga cgggcggaag
    15061 tccgttttcg gtgcattcgc cgcgcaccag cgatcgacgt acgtacggaa cggcgcgagc
    15121 agcaggttgt tgccgctgcc gtgcttgaag acgattgtgt cgagtgcccc gccgttgcgc
    15181 agatcgaact ccaggtgcca ataatcggtg gtgaccttga cgcggtcgcc gggtgaggtg
    15241 tcgagttgga tggaacctcc gcccggcgcc tgtatctgtg gcgcctgagc gaaagacacc
    15301 gtgcagaata gcgcggcagt acacaatcca cgaagccgtg aagtcatgag aggtcctttg
    15361 atagatgaag tcggtaccaa cggaattcta tatcgtgatc tgatagtctg caaacgagat
    15421 aaaacggagg gtcgttgatg aaagcttacg cgtgggtcgc agtttgcctg gcgtcggctc
    15481 tgttcctttc tcccgcttcc tcgcagcaga ggaaaccggt ccgagtctac tcgacgcttc
    15541 tcaatccgcg cgagcatccc gacgacgggc gccgccacgt acgcccgccg tcgtgggaga
    15601 cgttcggcca tcgcacgctc tttaccgctc tccgtgggtt tgaagtcaag gacggaaggc
    15661 tcgttcgcta caaggaggaa ctcgacaagt acacgcgcga gcacgagctt gccgaggtca
    15721 tctggccgtc ctaccccatc ctgttcgcga acaacctcgg cgacctcgcc gatgaaatcc
    15781 gcgaccggaa gctcttcctg ttcgacatat ggggttacgt gccaggctcc ggacccggcg
    15841 gctactggca acaattcgag ccgcctcccg gcgtcttcca gatgctcgaa tcgaagctcg
    15901 gggatcgctg gctcggcatg gacgtgggcg agcaggacgg ccgctacatc ggcggctacg
    15961 ctccccagat gtatccggtc tccgccgggc gcttcgacca gtatctgaac ttccaccgcc
    16021 atttcgagcg cttcaccagc gacctcggca accggatggc gacgcttgtc tccctcaact
    16081 tcgggcacta cttcttgaaa gagggaatct acaccctgat cggcgccgaa accgcccagg
    16141 cgctcccgaa tggccaggtc tactactcgt tcatccgcgg cgccggaaag cagtacggcg
    16201 taccatggtt cggcaacgct tccgtctgga accgctgggg ccacaagacc tattccgact
    16261 cgggctccga cttcggaccg accaagggca ccagtgtcag cctgctcaaa cggttgcttt
    16321 acagccacat cctctataac tccttgctgg ttggcttcga aagcgcgtgg ttcgatggcg
    16381 gaggcaagct gactcccatc ggccacgtgc aggaatcggc gaacaggtgg gtgaaacaaa
    16441 atggccagcc gggcgtgatg atgacgccga tcgcggtgat ggccgacttc ttctccggct
    16501 ggtcgtttcc ccggcacctt tacagccggc acgtctaccg cgtctggggc aacctgccat
    16561 acgagtccgg cgaccacctg accgacggcg tccttgacat gctctatccg ggctaccagg
    16621 attcctcgta cttccacgac gaacgcggct tcatcacaca gactccgtac ggcgacgccg
    16681 ccgatgcgct gctcagcgac gctccgggtt ggctgctcgc gcgctatccc gttgtggtcg
    16741 tggccggcga gttgtcgggc gacatcgaga cgcgcgacaa gcttgaggag tacgtgcggc
    16801 agggcggaca tctggtaatc accgcgggca acattgcgaa gtggaacggt gggatcggcg
    16861 gagtaacggc cgccggccca gcgcgcagct tcgcgccgaa gcaggcgctt caggttgccg
    16921 gctcgcgggt ggtggaggat cgggcgttcg aggccatcgg cttacgcctg caggctgtgc
    16981 acaaggtgct ggtccgttcc accggcaccc tcgccgcgga agtgcccctc ggcagaggcc
    17041 ggctcaccgc gctgggtagc ctgttcggcg tcccgaagga ggccgtgatt cagactgtgc
    17101 gcaatgagat cgacaagccg ctcgcaaagc cgtacccgct gttgaagcac gtgcggatga
    17161 tcctcgaccg cgtgtttcgc gagcaggtgc ttttcgatgc cggcgaggga ctgagcctga
    17221 tcgtatgccg gaaagaggcg ggcgaataca cgctcggcat cacgaacaac tcgtggcgcg
    17281 aaaggccgtt ccacatcgtt tcgcacatcg gcaagatcga gtcggtcaat gagttgccgc
    17341 tcgaccggtc ggaatggggc gccacgggtc acgtgccgga aggggtggat gcttccgcgc
    17401 ttggatcgag cacaaaccgg acgatcgccg gcggcgacat tcgcgtgctt cgcgtgcgcg
    17461 tccgggatga gaacgtcgag gagattcggc atgccgctcc gccgccgggg ccgcggggac
    17521 gggcactgcc gctgcgcggc gatggttcta tccaggaaca gatcctgagg cggccctctt
    17581 tcttccagca cttcgacacc gctgtgattg attggcggta tctgaacagc cgcgagactg
    17641 aggctctgaa caaggaagca cgctggctca agctccaggg actcaaggtg atcgtcgatc
    17701 tgacctcggg tctgaatctc tacccagacc tgcggatcat cgacaacatc ggctcggact
    17761 acgcggcgag catgagagcg atcgacagcg tgttgtcgaa gatgcagatc ctcgattcgc
    17821 gcgatttgat tctctcactg caccgcgtac cggagaacaa cttcacccgg gacgagacct
    17881 gggctgggtt cgagaagacc ctccggcgaa tctgcggaca ggcggagtcg gcgcggatta
    17941 ccgtgcattt gcgcatggcg ccgggcaagc cgccatccga cctgaaggag gcggccgcat
    18001 ttgtcgcgcg ggtcaatatg ccgaatctga aaatcgctcc gagtacggcg atgcttcttg
    18061 cggccaaaaa tgggccggaa gcgctttccg cggtgccgcc ggagaaggtc gggatgtggc
    18121 ttgtcgccac gcccgccagc gatatcggag gcagcgtgtg gcagtatcac ggaccggtcg
    18181 ccgggaagga tttggaagcg ctggtacggc cttttctggc cgccgccccc ggagtcccgc
    18241 tggtcctcga tgccgtctac ccggaccagg acgcggaata ccgtgacgcc cgcgcgcttg
    18301 accggctggc ggggaagtag gttggcagcg atcagtcttc agctctcagc agtcagccag
    18361 cgaggctgat cgctgagagc tgacagctga aggcttccct actccacccg gaacaggatg
    18421 ccggcgccgg gggcgagcca gtccatctcg cgtccgaact cttcttcgat gccggcgtag
    18481 ttgcagatgc ggatcagctt tttcccctgc tccttcagct caatcgagaa ttggaagctg
    18541 ttgttcaggt ccttgttcac gatcatgaag taggggcgac cctgcccgtc ctcgaactcg
    18601 ccgatcagga aacggccctg gatgggtggc ttcacgtagc gcatcaccat cggtacgctt
    18661 cggatcaact tgctctgcga aagcggcttg ccgaactcag gcacgtccgg atagtggtat
    18721 acgcccgtgc tcttgagctt cagcatcgtc ggcgcgagag cgtgaatctg gaggttgatc
    18781 cgccgcagcg cgtcccacgt cgccgtcttg ttgccgaact ggtcgaccgc gcccagccgg
    18841 tagttgccgt tgtgcggcga gtagtaagtg aagtactgga ttccgcgccc gccgtaggcc
    18901 agcgttgcgt acacctggag gttgaatgtc gcgtccgacg gctccatgta gttgaagtga
    18961 gcgttcgcca ggatgcagtt ccagaacggc gtattcgttt cctgtgaaat gcggcgtacc
    19021 gcctccaggt tgttgtagaa gtactccagc atctcgccgc cgaccaccga gtagttgtcg
    19081 tagctcagga acggctggcc gatcgtgtct accagcatct tggcgtaggt gtcgtagtcc
    19141 ggcgttccca tccgttcctt actcacgcgg tacggaaaca ggtttacgta gggccacttg
    19201 tcaggcgcgg cctccttgag catcttcgag acgcgtccca tcgccggcat cagcgaggcg
    19261 tgcggctcgt ctcgcaggta gtacccgaga acggctgggt tagaggcggt cgccttcacc
    19321 acctcggcga cgttcctccg aatctcggac tcggacggca tgttcgccca gtcgtacccg
    19381 ttgacccgtt tgtcggagac aaagcaggac agtccggcat cctggacctt ccctacgtcc
    19441 tccactcggc aaaagccgga tgcgttcagc ccggcgtctt tcatcaggtt gagctgcgcc
    19501 tggtcggacg gcgaacctcc ccaggccatg atcgggaagg tcttggcggc cacgcgctcg
    19561 gggttatgga actgctgctg gcccggcgcc gccgcggcca gaagaaacag gcacacgatt
    19621 accgttgaga ttgtgcgaat catgatgttc tccgtgtttt tgagttactg gtgcgcgccc
    19681 gcgaattcaa tctgcgcctc tcgcagcgat aggcgcgcca ccatcgcgtc catcccgatg
    19741 gccttctgcc gcgccgcggc cgccccttgg gtgtcgcctt tctcttcgag tacgagcgac
    19801 aagagataat aaccgatcgt ctccggcagg ccacgatatt cgtacttgcc ggacgtccgt
    19861 tcccgcgcct ccgcttcgag cttgcctagc agtgctttgt attcgccggt ctgcccgagc
    19921 gtcttgttcg cgagcatctg gtagtacatg ccgagatgcg ccttgccgta cttctcgtcg
    19981 aggatcttct tcaggtgctg ctgggccgtg tcccgcctgc ccatcgcctg gtagacgcgc
    20041 gccaggtccc aattgacgtg ggcctggaag tccggcgtcc gcgcggccac ctcgaggttc
    20101 ttcggatact cgcccgcctg ctgatagtgt gctagcgccg tctggtaatc cttccggtcg
    20161 aacgcggcgt cgccgagctg cttgttgacc tcgacccaag cgtagtggat ttcgtacttg
    20221 ccctcccagc tatggaagtg gtggttcttc agcacgtcgt agactgcctt caggttttcc
    20281 gtgccgccct gtttgagccg taagtcgatc agcgtcgaga tcaggtcgtc gcgggagttc
    20341 acggtcttcg ggtgcttcat gaacagtgcg tatctctccg cctcggacac cttgatctgc
    20401 tgggcgacgt catcgagttc cagcaggatc cggccgtccg tcgcgtcgac cgcgaacgcc
    20461 ttctggtagg cctcgtaaga cgcctgcatg tccttcttca gataccgctg cccataggcg
    20521 acattgcgct gggcgagcgg tgactgcggg ttcaactgga ccgctttctg ccagtcggca
    20581 aacccctcct ctcgttgccc actgccgtag agcaggttgc cgaggaacag gcgggcgttg
    20641 gcgtcgtccg ggtaggcccg gatggctgct tcgagcgccg ccttctcctc caaccggtgc
    20701 gggaaaacgt tgatcggcga tcctttgcgg gcgacggcat agaactgcgc cgccgttgtt
    20761 ttgtcaccgg tcagctcctt gaagtagccc cggaagtagc tcaccagcgg gctgatccgc
    20821 gtgacgtctt tgccctccgc atagcgcgcg agcacgtcgt cggcatccac gaactgaccg
    20881 gctccggcat aggcagccgc cagctcgaga tagttctgca cggcgtcacg catgtatccg
    20941 ctccagattg aaccccacgg atcggctgct ttgccagtgt tcttcaccgc gagcgtcttc
    21001 tcgtatccgc ccatgaagtt catcgcgtca agatcgagcg ccttttccgc cgccgtcagg
    21061 gcccgcttgt gatcgccgag atgccggtag gccgcggcct tcagagccgg aatgtcggcg
    21121 aacttgtcat tctgtttccc ggcttcgtcg agcttggcaa tcgcctcgcg gtaattgccg
    21181 tcgagcatgt cgatcttggc gagattcagg tatgcgggca cgaaatactc gtagccgtaa
    21241 atcgcgcggt agaactggtc gtaggcttcc ttgaacttcc gcagacccga cagcgccagg
    21301 cccttgccgt agtagagtcg agcgttgtcg ctgtcgcgct ccagcgctgt gttcaagtac
    21361 gtgagcgcct cgttccattt cccctgcttc agggccagga agcccatctc ggcgttgacg
    21421 cgcgaatcct tcggatcccg gctcagcgct tcgttgtagt acaccagcgc ctcgttcgtc
    21481 cggcggaacc ggtcaagcca ttcgccggcg tgatacagtt cgtccgggtt cattttcttc
    21541 ggatcgcccg gatccgtctg gcctggcggc aggtccacct tctgcggcgg cttctgctgg
    21601 agttcgatgg ccagtttgcc ctttgcgtcg tacaccgcca ggtgcaggtc gtaaaccgtc
    21661 gcgccgccag ccggcacttc caccgtgtat ggcttgtccg gcgaaatcgt ggctgccgct
    21721 tcgctcaaca ccttgcctgt cttggtgtcc ttcaacacca ccttgcagtt cgaaaagact
    21781 cccgtagcgt tgacgccgcc gaaggctttg ccgtctttca agtcggtgtt cacagcaaaa
    21841 tccttgctcg cgttgcgata gccgcggagg tcgcgcaccg gataccagta gtcgtgcgcc
    21901 gtcttcactt catacgcgcc catccagtgg tagtcgggct ggttgtcgct ccagacgccc
    21961 gcctgtggct cgtagtatgg cccggagccg tcggagagga tgtcgtccca gatgcggccg
    22021 gaggggccgg aaccccacgt ccagagtttc ttcccgagca cgatgtgatg gttgccgacg
    22081 tgcaccatgc cggcctgctt ctggtggtca taggttccga accactccga cgggttgttg
    22141 aacgcgaaga agctgctcgc gttcggcaca ttcttccacc acgtcaggtc cacgccgttg
    22201 tgaaccggcc agttccagaa ctcgtgcttg ctgtggcccg tcaccatgtc acccggatac
    22261 tgcgcctgca cgggatcgcc cgcgtaggtc gcggcggtgg cccagaactg gaaggagtgc
    22321 ttgtgctgtg tcgggttgat gaagacgtag tccgccttga tatagctacg gcccgggtac
    22381 accgtcatcc cgacaagcca gcgcatccgg aagatcggct ccgtctctcc gacccaaacc
    22441 gtcgcgctgc cgtccgcgtt cttcacaatt cggtggtcgg tgggcatgaa gcaggacgga
    22501 cggtggccgt gcgggaagtt ccactcgatg ccgccggaga tccacgcgcc agtcatgctg
    22561 atcaggccgg gcttaacggt cttctgccag tagaagaact cgaagccgtt ggttttatcc
    22621 aacgccccat gcaggcgccc gccgatctcc ggcagcacca gcaccttgat gtactcgttc
    22681 tcgaggtaga ccgcggtgta cgctttgtcg accttgttgt tggtcagcgt ctcctgcaag
    22741 ggatacgggt agatatcccg cccgccgtcg aggaataccg ggtgcttctg cggtggtccg
    22801 atctcccagg tgggcatggt gagtttttcc tcccaagcct ttacctggcc gaacgcgtgc
    22861 gcgccggtcg ccagtagata caatgtcagt gcgaaggtta agaagattct gctcatggat
    22921 gtgctccgga ggacgctgtc tctcccgcgt ccgaccacag tatatcagca cctggaggaa
    22981 ttcctgcccg agtgaaatcc gctctccggc gccggccccg aaagactcaa tccggcacga
    23041 aatcgagact tctcagacga attgcgcggt ggctgtcgat cggtctcgtt gccttcatcg
    23101 gactctgcgg cttgagcctg ctgctcatgc ggtggatcga cccgccgttc accgccgttc
    23161 acgccgaacg ccggatcgag tcccttttcc gaaagggtcg ctaccagaaa cgctaccggt
    23221 ttgtcccgct gcggcggatt tcggttccct tgcaacatgc ggtgatcgcc gccgaagacg
    23281 ggcggttcta tcagcactgg ggtcttgacg tgctggagat gaagaaggtc gtcgaagacg
    23341 attggacgcg aggccggatg cgaggcggtt ctacgatcac ccaacagctc gtcaagaacc
    23401 tcttcctcac gacgcgcgga tccgtaattc gaaaaggatt tgaattcgtt ctcgcaccgc
    23461 tggccgaagt gctgctgccg aaggaccgca ttctcgagct ctatttgaat gtgatcgagt
    23521 ggggacccgg tgtctatggc gcggaggccg ccgcgcggca gtactacgcg acatccgcgg
    23581 tcgcgatcgg ccgggagcag gcggcgcggc tagcggccat cattccatct ccacttcgga
    23641 gacggcccgg gcggatggac gactacagct ccgagattct ggagcgcatg agccggatgg
    23701 gctggtagcc cgttcagggt ggctggggcg ccgctccctc acgggtcgcg gttccttcat
    23761 ctctctgccc ccgagtatcc aggtcctttc acaaatcgtc ccggcttcgc tccggtgtgg
    23821 tctccttccc agaggacagc cacgccgttc acaaacacat attgcacgcc agtcgcgtac
    23881 tgatgcggtt tttcgaatgt tgcatgatcc tggatcgtgg ccgggtcgaa aaccaccacg
    23941 tccgcgtagt agtccgcctt caacattcca cggcgattga gcctgaggtt ctcagccgga
    24001 agcgcggaga gcttacgaac cgcctcttcc agtggaatga tcttctcgtc acgcacgtac
    24061 ttcccgagca ggcgcgcgaa gctgccgtac gccctgggat gcacgttact tttcaggaag
    24121 acgccctccg gcgcgagcga gggcgagtcc gagcaaaagc tgacccacgg cgtcgcgatc
    24181 gctttgcgca cgttgtcctc cgactgcgtg aagtacaccg tgccgatccg gctctcgtct
    24241 tcgaggatca gatccatcgc cgtctcctcg ggcgatttct tgcgcatccg ggccacctcg
    24301 ccaagagttt ttccagtgag cggcttcagc tccgccttcc ggaagtcgac gagcaggata
    24361 ttctccggcg atgccgcgcc gagatacatg ttctcccatt ggtccgactc gcgtttcatc
    24421 tcctgtacga ttcgttttcg cgtcgccgga tctttcaagc gcttgaggaa cgcttcgtag
    24481 ccgccctctt gcgtccacgg cggcatcacg gaatccaggc cggtcgagct cgcgggatag
    24541 gtgtagatgt ccgccgtgac gtgcaggccc tgggcacggg cctggtcaac tttggcgatc
    24601 gcttcggtca tctttggcca gttcgctttg ccggaggctt tcaggtggta gatctccgcg
    24661 cggatctttg cctcgcgcgc gatcctcagc aattcatcga cggatggcag aagctgggag
    24721 ccttcgctcc gcaagtgcga gatatagatg ccgcccgact ccgcggcgac cttggccagt
    24781 tcgatcagtt cctcggtttt ggcgaagttg cccggcggat agatcagcga ggaggcaatg
    24841 cccagcgcgc cttcccgcat cgcctcgcgc acgagttggc gcatcttctc gagctcttcc
    24901 ggcttgggcg cccgaattgc gtgcccgatc acgtaaatcc gcggcgttgc cgc
//
LOCUS       DATIFQ010000134         7677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_618695, whole genome shotgun sequence.
ACCESSION   DATIFQ010000134 DATIFQ010000000
VERSION     DATIFQ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618695"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..141
                     /locus_tag="VLE22_06055"
     tRNA            52..141
                     /locus_tag="VLE22_06055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:86..88,aa:Ser,seq:cga)
     gene            647..1264
                     /locus_tag="VLE22_06060"
     CDS             647..1264
                     /locus_tag="VLE22_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14002.1"
                     /translation="MEQEESTGSRLYLSNCLAWFAFLLLGVSVVPAPTVGQKAPAGLT
                     MHRQQAGTPNAKGWYDAKSTEGHFSVSLPIPFNDFTVKVKEKDGTEVVSYVIGSTSKE
                     GFKFSATEMLNGRRTREFDADAYMEKFKADKRNTVSNETLFEVDGHSAIQFHVSNAQQ
                     SAFMRAIVVPSGVLLLIVEYPTRLERDVISLVKHFFDSLKIEQIM"
     gene            1499..2329
                     /locus_tag="VLE22_06065"
     CDS             1499..2329
                     /locus_tag="VLE22_06065"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB14003.1"
                     /translation="MHSHRVVTGCVSLLLLPVLVCAQEAVTPAETIHLFNGKDLSNFY
                     TWLVNTKYQDPDRVFSVVEAIDGVPAIRISGQRWGTLATKREYANYRLIMEYRWGVLT
                     WAQRKNGVRDSGILLHGQGRDGNSKADFNGPWMRSIECQVGEGIVGDFIVVGGFEEDG
                     QRLVPKLTVKVRKAPSGELFYDPDGVEREVGRVAWSGRDPDWEDILGFRGRNDVESPW
                     NRWTKLEVICEGDSITHIVNGKIVNVGTRSSLTRGKILIQSEGAEMYLRRFDLEPVTR
                     "
     gene            complement(2373..3119)
                     /locus_tag="VLE22_06070"
     CDS             complement(2373..3119)
                     /locus_tag="VLE22_06070"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HSB14004.1"
                     /translation="MNRFVGVDLGWYGKPSGLASLEWNGRELIQRKLDRLERPDEILG
                     WIDSETGQENAVVAVDAPLVIANETGIRPAERDLNRDFRRFHAGCHPANLSRPFAPHV
                     IEFSHRLESLGFAHGTSIQPRQDGRFQVEVHPHAATITLFGLDRIVKYKRGPRQQRAR
                     ELRRLRTLILKRLSVADPTLHARLPGIPRAGSTKPAEDKIDAVLSAYIAAHWWFWATQ
                     RNRTYGSAQSGYIVVPNPHQGGRFQSNSTA"
     gene            complement(3116..3817)
                     /locus_tag="VLE22_06075"
     CDS             complement(3116..3817)
                     /locus_tag="VLE22_06075"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HSB14005.1"
                     /translation="MKSWSGSRECVADPSRDTNVRIKEIWRYPVKSMAGEMLQTAELS
                     STGIAGDRIIQVRNATGRIMTARTRPLLLRHRAELRPGDQILIDGQPWRSPEIARRVA
                     AAAGPGTRLVMSGAQDRFDVLPLLVVTDGMLEAVGYDRRRFRPNLVIGGVPGLSERQW
                     EGTQLRIGEVVIGLEDLRGRCIMTTYDPDSGEQDLNVLRKVQKQFDGRLGLNSYVVTP
                     GRIAVGDPVEWIKSR"
     gene            complement(3814..4686)
                     /locus_tag="VLE22_06080"
     CDS             complement(3814..4686)
                     /locus_tag="VLE22_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB14006.1"
                     /translation="MYYRYANVDGLQVFYREAGDPKNPAMVLLHGFPSSSHMYRNIIP
                     KLADKFYLIAPDYPGFGNTSAPSPKTFPYTFDKLSEVMEKLLISLGVKKFTLFIQDYG
                     SPVGFRIALRHPEWIEGLVCQNGNAYEEGFTGVWDALRSALWKNRTPATEAPLAAFFT
                     PDGVKWIYSEGTKDPAKISPDNWNTDLFFLGRPENHRIQLDLFYDYRTNVDQYPRWHE
                     YLRKHQPPTLLVWGANDPIFTQEGGRAFARDLKTPELHMLDTGHFALEDHCDEIVGLV
                     RSFYDRRITVKKAA"
     gene            4868..6430
                     /locus_tag="VLE22_06085"
     CDS             4868..6430
                     /locus_tag="VLE22_06085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSB14007.1"
                     /translation="MDLHSVILSIAQAEGPPSVLGQIIKAIMANEHVALARIWFLDQD
                     GACLECGESGEETALHMRASGGRPLAPGADWSRIDGAHHRVPLDSERKLAQMARTAEP
                     VLIRDIQGIHDAVMDREWARHEHIEGFVGHPLLFRGSVVGVLAVFLRVHPNQATLEWL
                     STFAAHAAVAIGNCRAFQQIDRLRRQLEQERDYLREEVVETGEFRDIIGHSPALMRVL
                     QQIDLVAQTDSSVLIQGESGTGKELVGRAIHQRSRRAAKPLIRVNCGSIPKDLFESEF
                     FGHVKGSFTGAIRDRIGRFQLADGGTLFLDEIGEIPADLQSKLLRVLQEGEFERVGDE
                     HTRKVNVRVIAATNRDLLRESEAGRFRSDLFYRLSVFPLEVPALRDRREDIAVLAAHF
                     VRQACARLHMPEAKLPRRELDRLINYPWPGNVRELQNVVERAVILAHGGNLTFDLGSA
                     RPPITAPHDETRFYTEAQWRDMERENLLRALKQAGGKVSGAGGAADLLGLNPNTLASR
                     LRTLGISRGFGG"
     gene            complement(6892..7020)
                     /locus_tag="VLE22_06090"
     CDS             complement(6892..7020)
                     /locus_tag="VLE22_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14008.1"
                     /translation="MQLLVQILSVRILLYGEHFFEIRYDEVFVLTARLLLTASCPD"
BASE COUNT         1583 a         2248 c         2334 g         1512 t
ORIGIN      
        1 cgcatccagt cggctatagt ggagaaagag acctaccttc cacatcgagc cggtgaggtg
       61 cgagagcggt tgaatcgggc ggtctcgaaa accgttgaac ctttacgggt tccgtgggtt
      121 cgaatcccac cctcaccgcc atcataagtt cttttgagtc aatcaattga cgacctcctg
      181 tttctcgttg tgactctggt gtacccttcg catggacgaa cacgattagg ctgcggtccc
      241 aagtccggag atgacgtggc gacactcttg aagcatggcg cggcggatgg aggctctacc
      301 ggtgccagcc gggtagatcg cggcaccggg caagaaggcg ggtcaaagga aagagggtca
      361 aaggaaagag ggggagaggg agagccacat aagaagtctc ttgagataag acacttacgg
      421 attcctgcgg caggacaatc cagcacggct gggagatcga ccacgtcaaa cctgtttcgg
      481 aaagcggcac ggatgatctc tccaatctcc aaccgctgca ctgggagaac agccgggcaa
      541 agggcgatga ctggccgcgc tggacctgca cgaaacgccg ttgaatgggc tgggtgtaga
      601 atagagtcaa tctcgctgaa gccgccccga actcggagta gtgaccgtgg agcaagagga
      661 atcgacaggc agccgccttt acctttcgaa ttgtttagcc tggttcgcct ttctgcttct
      721 cggtgtcagc gtcgttccgg ctccgactgt gggccaaaaa gcacctgctg gcctgacgat
      781 gcaccggcaa caggcgggca ctcccaatgc aaaaggttgg tatgatgcca aatcgaccga
      841 gggccacttc agcgtatccc tgccgatccc cttcaacgat ttcaccgtaa aggtcaagga
      901 gaaggacggg acggaagtcg tgagttacgt gatagggtcg accagcaagg aaggcttcaa
      961 gttctcggcg actgaaatgc tgaatgggcg caggacccgt gagttcgatg ccgacgcata
     1021 tatggagaag ttcaaggccg ataagagaaa tactgtctcc aatgaaacac tatttgaggt
     1081 cgacggtcat tcggccattc aattccatgt ctcgaacgcc cagcagtctg ccttcatgcg
     1141 tgcgattgtc gttccgtctg gcgtgttgct cttaatcgtc gagtacccca cgaggttgga
     1201 gagggacgtg atttcccttg tgaagcattt cttcgattcg ttgaagatcg agcagattat
     1261 gtaagagacg cgtcctgaac gggagtcacc cggactggtg tcagtgacgg gccgcaggat
     1321 ttcacggaac cgattgccac gccgtttgta tcagccagcg cgacgttgcg gattgtctgg
     1381 gataggcggt gcaggttagc cgggatgctg gagcggacta ccgtacgaat cccccgaccg
     1441 caagagagaa gcgatttttg tgggctgtgc acgtaatgtt attaaagagc gaaaggccat
     1501 gcactcacat cgcgtagtga ctggatgcgt ttccctgcta ctgttgcccg tcctggtgtg
     1561 cgctcaagaa gccgtcacgc cggccgagac gatccacctc ttcaacggga aggacttgtc
     1621 gaacttctac acgtggctgg tgaatacaaa gtatcaggac ccagaccgtg tcttcagtgt
     1681 tgtcgaggcg atcgacggag tcccggccat tcggatcagc ggtcagcggt gggggacgct
     1741 ggccacgaag cgggagtatg ccaactaccg actcatcatg gaataccgct ggggcgtgtt
     1801 gacctgggca cagaggaaga atggtgtccg cgacagcggc atcctgctgc atggacaggg
     1861 ccgcgacggc aacagcaagg ccgatttcaa tggtccgtgg atgcggtcga tcgaatgcca
     1921 ggtcggtgag ggcatcgtcg gcgacttcat cgtggtcggc ggatttgagg aggacggcca
     1981 gcgtttagtg ccaaagttga cggtgaaagt caggaaggcg ccgagtgggg aactgtttta
     2041 tgatcccgat ggcgtggagc gtgaggtagg ccgcgtggct tggtcgggcc gcgatccaga
     2101 ctgggaggac atcctcggct ttcggggcag gaatgatgtc gagagcccct ggaatcgctg
     2161 gaccaagctt gaggtgattt gcgagggcga cagcatcacc catatcgtaa acggcaagat
     2221 tgtgaacgtg ggtacacgtt caagcctcac gagagggaag atcctcattc agtcggaggg
     2281 cgccgagatg tacctccgcc gcttcgacct cgagccggtg actcggtaga gccgggcaag
     2341 ggcgcgctcg tatggtctgg aaactgcatc gcctaagcgg tggagttcga ttgaaaccta
     2401 ccgccctgat gagggttggg caccacaatg tacccgctct gcgcggagcc gtacgttctg
     2461 tttcgttgcg tcgcccagaa ccaccagtgg gctgcaatgt aggcgctgag aacagcgtcg
     2521 attttgtcct cagcgggctt ggtgcttcca gcacgtggaa tgcccgggag ccgtgcgtgg
     2581 agcgtcgggt cggcaacaga aagccgcttg agaatcagag tgcgcagccg gcgcagttcc
     2641 cgtgctcttt gttggcgcgg gccacgcttg tacttgacga tccgatcgag accgaacagg
     2701 gtgatcgtgg cggcgtgcgg gtggacttcg acttgaaaac gcccgtcctg ccgcggctgg
     2761 attgacgtgc cgtgtgcgaa gccgagggac tccaaccgat ggctgaactc gatgacgtga
     2821 ggggcgaaag ggcggcttag attcgcggga tggcatccag cgtgaaaacg ccggaagtcg
     2881 cggttgagat ctcgctccgc gggccggata ccggtttcgt ttgcgatcac caagggcgcg
     2941 tccaccgcaa caacggcgtt ctcttgtccg gtttccgagt caatccatcc gagaatctcg
     3001 tccggccgct ccaggcggtc gagcttccgt tgaatcaact cgcggccgtt ccactcgagc
     3061 gacgcgagac cgctgggctt cccgtaccag ccgagatcca cacccacgaa gcgattcatc
     3121 gagatttgat ccactctacc ggatcaccca ccgcgatccg gcccggcgtc acgacgtagc
     3181 tgttgagccc caagcgccca tcaaattgtt tctgtacctt ccgcagcacg ttcagatcct
     3241 gctcgcccga gtccgggtca taggttgtca tgatgcaccg accacgaagg tcttccagcc
     3301 cgatcaccac ttcgccgata cgcagttgcg taccttccca ttgccgttcc gaaagacccg
     3361 gaacgccacc gatcacgaga ttcggtcgaa accgccgccg gtcatagccc acggcctcca
     3421 gcatcccgtc tgtcacgacc aacagcggca acacgtcgaa gcggtcttgc gcgccgctca
     3481 tcaccaggcg cgttcccgga cccgccgccg cagccacccg gcgcgcgatc tcgggcgatc
     3541 tccacggctg gccgtcgatc agtatctgat cgccggggcg cagttccgcc cgatggcgta
     3601 gcagtagcgg tcgcgtcctc gcggtcatga tccgtcccgt ggcgttccgc acctgaatga
     3661 tccggtcgcc ggcgattccc gtggaagaaa gctccgcagt ttgcagcatc tcccctgcca
     3721 tcgatttcac cggataccgc cagatttcct tgatccgcac gttagtgtcc cggctcggat
     3781 cggcgacgca ctcgcgcgag ccgctccaac tcttcaagcg gccttcttca ccgtgatgcg
     3841 ccggtcgtag aaagaacgca ccagccccac gatctcgtcg caatggtctt cgagagcaaa
     3901 gtgccccgtg tcgagcatgt gcagctccgg cgttttcagg tcgcgcgcaa acgcccggcc
     3961 accctcctgc gtgaagatgg ggtcgttcgc tccccacacc agcagcgtcg gcggctgatg
     4021 cttgcgcaga tactcgtgcc agcgcggata ctgatccacg ttggtgcgat agtcgtagaa
     4081 gaggtcgagc tggatacgat ggttctccgg ccggcccagg aagaaaagat ccgtgttcca
     4141 gttgtccggg ctgattttcg cggggtcctt cgtaccttca ctatagatcc acttcacccc
     4201 gtccggcgtg aagaacgccg ccagtggagc ctcggtcgcc ggcgtgcgat tcttccacag
     4261 cgcggagcgc agcgcgtccc acacgcccgt gaacccctct tcgtaggcgt tgccgttctg
     4321 gcaaacgagg ccttcgatcc actcgggatg gcgcagggcg atgcggaagc cgacgggcga
     4381 gccgtagtcc tgaatgaaca gcgtgaactt cttcacgccg agtgagatga gtagtttttc
     4441 catcacctcc gagagcttgt cgaacgtgta ggggaacgtt ttgggagagg gcgcactggt
     4501 gttgccgaaa cccggatagt ccggcgcgat caggtaaaac ttgtccgcca gtttcgggat
     4561 gatgttgcga tacatgtgag aagacgaagg aaaaccgtgc aacagcacca ttgccgggtt
     4621 ctttggatcg cccgcttcgc gataaaacac ttgcaagcca tccacattcg cgtatctgta
     4681 atacatggct gagttccttt ctgttgtggg ttgacacccg cggagggatg gtgcacggac
     4741 ctagccgcag tactgatggc atctaatcaa tgagttgcag gaaaacggcg cagcctgttc
     4801 tcatgatcca tcacgagacg ggaataaggc gccacgaatt cgtgatttct aacgagaggg
     4861 acgcttgatg gatcttcatt ccgtcatcct ttcgattgca caggctgaag ggccgccttc
     4921 cgtgctcggc cagatcatca aggcgatcat ggccaacgaa catgtggcgc tcgcgcgcat
     4981 ctggtttctc gaccaggacg gagcgtgcct cgagtgcggc gaatcgggcg aggagaccgc
     5041 gctccacatg cgcgccagcg gaggccgtcc gctcgcgccc ggcgccgact ggagccgcat
     5101 cgacggcgcc caccaccgcg tgcccctcga ctcggagcga aagctcgcac agatggcgcg
     5161 tacggccgag ccggtgctga tccgtgatat ccaggggatt catgacgccg tgatggaccg
     5221 cgaatgggca cggcacgaac acatcgaggg cttcgtgggc catccgctgc tatttcgcgg
     5281 cagcgttgtc ggagtgcttg ccgtgttcct gcgcgtgcat ccgaaccaag cgacgctcga
     5341 atggctgagc acctttgctg cccacgccgc agtcgcgatc ggcaactgcc gcgcgttcca
     5401 gcagatcgac cggctgcgcc gccagctcga acaggagcgc gactacttgc gcgaggaggt
     5461 ggtcgagacc ggcgagttcc gggacattat cggccacagt cccgcgctga tgcgtgtgct
     5521 ccaacagatc gacctcgtgg cccagaccga ttcgagcgtc ttgattcaag gcgagtcggg
     5581 caccggcaag gaactcgtcg ggcgcgcaat tcaccaacgc agccgccgag cggcgaaacc
     5641 gctgatccgg gtgaactgcg gatcgattcc aaaggatctc ttcgagagcg agttctttgg
     5701 acacgtgaag ggatcgttca ccggcgcgat ccgcgatcgc atcgggcgct ttcagctcgc
     5761 cgacggcggt acgctgtttc tagacgagat cggcgagatc ccggccgatc tgcaatcgaa
     5821 gctgctgcgc gtgctgcagg agggtgagtt cgagcgagtt ggcgacgagc acacccgcaa
     5881 agtaaacgtg cgcgtcatcg cagccacgaa tcgggacttg ctgagggagt cagaggccgg
     5941 ccgtttccga tcggacctct tctatcggct cagtgtgttt ccgctggagg tcccggcgct
     6001 ccgcgaccgc cgcgaggaca tcgccgtact cgcagcgcac ttcgtccgcc aggcctgcgc
     6061 ccggctccac atgccggaag caaagctccc tcgccgtgaa ctcgaccgcc ttattaacta
     6121 tccttggccg ggcaacgtgc gcgagttgca gaacgtcgtc gaacgcgccg tgattctcgc
     6181 gcacggcgga aatctcacct tcgatctcgg ctccgctcgc ccgccgatca cggcgcctca
     6241 cgacgaaacc cgcttttaca cggaagcgca gtggcgggac atggaacgcg agaacctcct
     6301 tcgggcgttg aagcaggctg gcggcaaggt ttccggcgcc ggcggggcgg cggacctgct
     6361 tgggctcaac cccaacacgc tggcgtcacg acttcgaaca ctcggaatct cgcgcgggtt
     6421 tggcggctag tccgaggacg aatcccccct cacggccaga cttgatgctc ccaattcccc
     6481 gcccagcagc ccgggagcca tcggacgcca ctcgaaccca taatgccaac tgtgcctaat
     6541 cttatgcatg cggctgcggt cctggcgtat attaagcgga aatggatttc gttagcctga
     6601 ggccctggat cgaacacaac cagcccctca tcaacttcgg cctgctgatc ggagcggtcg
     6661 tgtacaccgt cgtgacaggt tggattctcc ggatcaatgt tcgccagatg agagtgacgg
     6721 ttcagccggc gttgacgctg aagagggagc gccgatcaga tggaagccga gagccaccat
     6781 atctaacgag tggatctctg gtcttttgca acacagggaa tgggacgggc ttaaacgtct
     6841 cctcaaccat ctatgtgcat tcgggcagcg gactggcttg gatgccgaag ttcagtcggg
     6901 acaggatgcg gtcagcagta gccgtgcagt caggacgaag acttcgtcgt agcgaatctc
     6961 gaaaaagtgt tctccgtaga gcagaattcg tacggagagg atctgtacaa gcagctgcac
     7021 gagtacgaag tgtttctgag atatgccagc attgtgggta cgcgctatct cacctatgtt
     7081 tcggccggta tggccggaga gatccgagct gactatgttg gcacggactc attgatgcaa
     7141 cgcctcctga tctggatcgg cgtcgcatgg cggtattacg cggtccacat cccgatttgg
     7201 agaaaactgc gtaggcagaa aaagcagaag caacacacct aaagggctgg ccacaaacct
     7261 cgaacagccc tgcccgactt atccacagtt gaccgccagc gcagatgccg aatagtaggg
     7321 ggtgtctcta atgccgagag acacaagctt ttgtcggata atgcccatcg aaagacaaat
     7381 gagcagctta ggcccctccc gccacctcaa agagcttggg gtgaagcagg agaaacccct
     7441 cccaggtcga gtcgtcccac cgttttcttt cttcaagggt ttccacgtag tcgactttgg
     7501 caccacgcct ccaacgtatt gaaaacaggg caacgtgttt agaatgggcc gcggtcgcga
     7561 tagcgaatcc caccctcacc gccagctatc cgaagacaaa acaatctgca tccatgctct
     7621 tcggcaattc cacgccactc ggttgtgttc cacgtgccgc tgaaagcggc cgacgct
//
LOCUS       DATIFQ010000135        12590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_427397, whole genome shotgun sequence.
ACCESSION   DATIFQ010000135 DATIFQ010000000
VERSION     DATIFQ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427397"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VLE22_06095"
     CDS             complement(<1..1304)
                     /locus_tag="VLE22_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HSB14009.1"
                     /translation="MSTSTVEPSTDAGTNYLNAGYGIKSWLLTKDHKRIALLYLAAIT
                     FFFFIGGAFAVLIRLELITPAGDLMGAETYNKAFTMHGVMMIFFFLIPSIPAVLGNFL
                     IPIMIGARDLAFPRLNLLSWYIYIAGGLFTLYAMAAGGVDTGWTFYTPYSSTYSNTHV
                     IATALGVFITGFSSILTGLNFVVTIHKMRAPGLTWFRLPLFVWAHYATSLIQILGTPV
                     VAIVIVLVGLERLFRIGIFDPKLGGDPILFQHLFWFYSHPAVYIMILPGMGVISELIT
                     CFSRKKIFGYNFIAMSSVAIAVLGFLVWGHHMFVSGQSMYAGMVFSVLSFLVAIPSAI
                     KVFNWTATLYKGSISYHTPLLYALGFIGLFTVGGLTGLFLATLAVDVHVHDTYFVVAH
                     FHYIMVGGMLMAYLGGIHFWWPKMFGRMYPELLARFSAVVIFV"
     gene            complement(1301..2254)
                     /gene="coxB"
                     /locus_tag="VLE22_06100"
     CDS             complement(1301..2254)
                     /gene="coxB"
                     /locus_tag="VLE22_06100"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HSB14010.1"
                     /translation="MWTDFQLFPEKASSIAAGVDNLYFFLVAVGVFFSTLIFATIFVF
                     AIKYRRRRPDEIPRPIHGSTLLEIFWSVTPGMIAMIMFGWGAYVYFRNYHPPEGAMEI
                     YVVGKQWMWKLQHPEGHREINELHIPAGRPVKLTMTTEDVIHSFFVPAFRVKRDVVPG
                     MYTSMWFQANKPGKYHLFCAEYCGNQHSGMIGWVYVMDPTDYENWLSGTSTGETMVQA
                     GERLFQRLGCHTCHMQDASGRGPALEGVFGSTVLLETGQKVVVDEAYLRESVLRPGAK
                     VAAGYKIQMPTFQGQISEEGLLQVVAYIKSLAPKKRVETQQ"
     gene            complement(2263..3057)
                     /locus_tag="VLE22_06105"
     CDS             complement(2263..3057)
                     /locus_tag="VLE22_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HSB14011.1"
                     /translation="MLRALVFVFAGILLGVAAQAQTTGMPEPLSKVGIDQRLNEQAPL
                     DLTFRDETGKQVQLRQYFGRKPVVLSLVYYECPMLCTMVLNGLLRSLRATPLNVHEHF
                     EVVTVSFDPKEGPELAAPKKAEYVKKYGRKGGSEGWHFLTGDEDSIKKLAQSVGFRYA
                     WDAQTNQWAHASGIMVLTPQGKIARYLYGVEYSAKDLRFALVEASAGRILSPVDQLLL
                     LCYHYDPKTGKYGFVIMNALRAAGMATVFLLGLFMFVNLRRDRRKT"
     gene            complement(3067..3435)
                     /locus_tag="VLE22_06110"
     CDS             complement(3067..3435)
                     /locus_tag="VLE22_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14012.1"
                     /translation="MKNYEPQDANIRGVVIFGIGLTALVAVALLLMGGLFRFLAERKE
                     PGPVPSPLALERTVPPEPRLQVTPNTDIEKLHAAGQAALNSYGWVDRQAGVVRIPIER
                     AIELTARRGLPVRAQAQEKK"
     gene            complement(3432..4589)
                     /locus_tag="VLE22_06115"
     CDS             complement(3432..4589)
                     /locus_tag="VLE22_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14013.1"
                     /translation="MARTEDFKRAQKLALAFGGAGLAVCALGAAFNPAQFFRSYLVAY
                     LFWAGIALGCFSVVMLHHLVGGRWGVMIRRLLESGTRTFPLMALLLVPLLFGLPHLYL
                     WARPEVVAHDELLQHKSPYLNVPFFLARSAFYFAVWLILSYFLNKWSAEQDRTPTASV
                     KSRLQYLSGPGLVLYGLTATFAAVDWVMSLEPHWFSTIYGAVFIVGQVLATLAFVIVA
                     LTLLSGREPMKGVLRPSHFHDLGNLMLAFVMLWAYVAFSQFLIIWSGNLPEETPWYVR
                     RLSGGWGWIAGILIGFHFILPFFLLLVREAKRRAGVLAILACAMLVLRLVDLFWVVAP
                     AFHENRIQVHWMDLAAPVGVGGIWLAVFLSQLKRRPLLPAAEFPLEGGRPA"
     gene            complement(4598..5218)
                     /locus_tag="VLE22_06120"
     CDS             complement(4598..5218)
                     /locus_tag="VLE22_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB14014.1"
                     /translation="MSFREMNHKGTKNTKESGVLCVFCVAVVIFSAGCRQDMHDQPRY
                     KPLGASSFFADGRASRLQVEGTVARGQLREDELYYTGKTGNDPAAVLPFPATREVLER
                     GRERYGIYCTPCHGLLGDGEGMIVQRGLRHPPSFHSDRLREAPVGHYFDVMTNGFGAM
                     ASYASRIPTADRWAIVAYVRALQLSQNATLEDAPASEQKRLMEGAK"
     gene            complement(5208..5744)
                     /locus_tag="VLE22_06125"
     CDS             complement(5208..5744)
                     /locus_tag="VLE22_06125"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HSB14015.1"
                     /translation="MERRRLYGVIAQFESPGDLLAAAQRAYAEGYRKMDAYTPMPIHG
                     LAEAIGFRRNYLPLLVLIGGLFGCGAAYLMLWSIETIIYPLNVGGKPFNSWPAFIPIT
                     FETTVLFAALAAVFGMLALNGLPMPYHPVFNVPSFALASNDRFFLCIEAKDPKFDYEA
                     TRKFLESQHSREVSDVEL"
     gene            complement(5749..7152)
                     /gene="nrfD"
                     /locus_tag="VLE22_06130"
     CDS             complement(5749..7152)
                     /gene="nrfD"
                     /locus_tag="VLE22_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HSB14016.1"
                     /translation="MEAEQRQDPGAAAVAATRAGEVPVIGPGHTYASVTGKISEIVLT
                     TKTPRGWIIGFAISFLLTMMLLFTIASLLVKGVGLWGINIPVGWGFDIINFVWWIGIG
                     HAGTLISAILLLLRQEWRTSINRFAEAMTLFAVACAGLYPLMHMGRPWLGYWLLPYPN
                     TMGTWPQPRSPLVWDVFAVSTYATVSLLFWFVGLIPDLATLRDRTKSRPLRVVYGMLS
                     MGWRGSAIHWQRYETAYLLLAGLATPLVVSVHTVVSFDFAIGIIPGWHTTVFPPYFVA
                     GAIFAGFAMVLTLAIPLRKFYRLEDFITMRHIDNMGKILLATGLIVGYGYLMETFMAW
                     YSGSQYEQYMISNRMNGPYQGYYWALIFCNILTPQLLWFSRFRRNLLALWLISLVVGV
                     GMWLERFVIIVVSLHRDFLPSSWGMYHGTKYDWSMYIGTIGFFLALMFLFIRVLPMIS
                     IFEMRTLVPEPDGKAKH"
     gene            complement(7157..10126)
                     /locus_tag="VLE22_06135"
     CDS             complement(7157..10126)
                     /locus_tag="VLE22_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HSB14017.1"
                     /translation="MMQPKNSLLDLSAIRARLENARGEEYWRSLDEVAQTAEFQAFVD
                     NEFTPGTSEWKNPLGRRDLLKLLGASIAFAGLTACTKQPPEKIVPYVRAPEDMIPGKP
                     MYFATAMTLGGYAAGVLVESHMGRPTKIEGNPEHPASLGATDAFAQASILTLYDPDRS
                     QVVLRNGLISNWAAFLAEVGLLREGLLAKEGAGMRILTETVTSPTLAQQLKDLLAEFP
                     EARWHQYEPAARDSAVGGAKLAFGEPVNTIHRIGQADVILSLDADFLASGPGSVRYAR
                     EFAARRREGGDRMNRLYIVEAAPSSTGSIADHRLPLRASDVEPFAWAVAAALGVAVPA
                     PGSPAPSGWVEAVAADLKKHAGSSLVIAGDQQPVAVHALAHAINAALGNAGKTVVYTE
                     PVEAAPVEQGESMRQLAEDMRAGRVETLVIIGGNPVYNAPADCEFRQGLLKVKRRIRL
                     GLYDDETSGLCHWHLAETHYLEGWGDARAFDGTVTIQQPLIAPLYGGKSAHEVLAVLS
                     GKGELAGHDIIKEYWRGRLGEAGFEKTWKTALHDGLIAGTAVAPKNVSVRKDFAAALP
                     APKGQEGMELCLRPDPTIWDGRFANNGWLQELPKPLTKLTWDNTVLISPATAERLGVA
                     NGQVLELRRAGRTVRGAAWILPGHAENSATVHLGYGRTRGGKIAEGPGFSAYPLRTSD
                     APWGGGGLAVSKTGATYKLACTQDHHSMEGRDLVREATVDEYRKDPHFAQHVVHGPVK
                     ELSLYKEFKYEGHSWGMAVDLNACTGCNACVVACQAENNIPVVGKTEVARGREMQWIR
                     IDRYFKGGLDDPEVCHQPMMCHHCDHAPCEVVCPTGATNHSSEGLNQMVYNRCVGTRY
                     CSNNCPYKVRRFNFFLYSDWDTKSLEPMRNPDVTVRSRGVMEKCTYCVQRINSARIES
                     EKEGRRIRDGEVVTACQAVCPTQAIVFGDMNDPNSRVAKLKKDPLNYGILDELNTKPR
                     TTYLARLRNPNPELKKG"
     gene            complement(10123..10731)
                     /locus_tag="VLE22_06140"
     CDS             complement(10123..10731)
                     /locus_tag="VLE22_06140"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSB14018.1"
                     /translation="MSIFGALFIGGGLLWVLNEVNRSPYVTDVGVALDQPVPFSHKHH
                     VAGLGIDCRYCHSTVEISSSAGMPPTETCMSCHSQIWADSPMLEPVRASFRNDQSIRW
                     TRVHDLPGFVYFNHSIHVKKGIGCATCHGRVDQMPLMWKTNTLYMEWCLECHRQPERF
                     VRPRGQVFNMAYRAPSDQLALGRKLVEEYKIRKLTDCSVCHR"
     gene            complement(11015..11194)
                     /locus_tag="VLE22_06145"
     CDS             complement(11015..11194)
                     /locus_tag="VLE22_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSB14019.1"
                     /translation="MPLYEYRCGNCGRTFEKIRRMADADRVMQCPHCESEAIERLLSA
                     FATGGCGGGGKSRFT"
     gene            11279..>12590
                     /locus_tag="VLE22_06150"
     CDS             11279..>12590
                     /locus_tag="VLE22_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HSB14020.1"
                     /translation="MRRIGLLALFLAALVPAAQDKFEYWPGAVYDPAIPTFRKVLGYE
                     TGERISWHANLVKYLEALAAAAPDRVKVFEYGKTWEGRRLVCAAISAKENIRRLDQIR
                     SAMQKLADPRRTPEAEAGKLMAGLPSVIWLAYGVHGNELSSPEASLLTAYHLLAARND
                     KMVQDILANVVVLIDPDQNPDGHDRFVHNFEQNEGIEPDPSPLAAEHSEPWPSGRVNH
                     YFFDLNRDWFALTQSETRGRVRLMLEWFPQVCVDLHEMGADSTYYFAPGALPFNPHLT
                     KFQREGFDIFGKNNARWFDQFGFSYFIREVFDEFFPGYGTSWPSLHGAIAMTYEQASS
                     RGLLMRRTDEMLLRFRDTVRNHFVASLSTAEASARNREKLLGEFYQYRSTAIQEGSTE
                     PVREYILPARGDKSAVDKLAALMVSLGAEVKRASAPFRNGDKEFP"
BASE COUNT         2496 a         4131 c         3656 g         2307 t
ORIGIN      
        1 acgaagatca cgaccgctga gaagcgcgcg agcagctccg gatacatgcg accgaacatc
       61 ttcggccacc agaagtggat gccgcccagg taggccatca gcatcccgcc caccatgatg
      121 tagtggaagt gcgccaccac gaagtacgtg tcgtggacgt gtacgtccac ggcgagggtc
      181 gccaggaaca gtccggtcag cccgccgacc gtaaacagcc cgatgaatcc caacgcatac
      241 agcagcggtg tgtgatagga gatcgacccc ttgtagagcg tcgccgtcca gttgaacacc
      301 ttgatcgccg aggggatcgc caccaggaag ctcagcaccg agaacaccat gcccgcgtac
      361 atggactgcc cgctcacgaa catatggtgg ccccagacca gaaagcccag taccgcaatg
      421 gccacgctgg acatcgcgat gaagttatac ccgaagatct tcttccgcga gaagcaggtg
      481 atcagttcgc tgatcacgcc catgcccggc aggatcatga tgtacaccgc agggtgcgag
      541 tagaaccaga agaggtgctg gaacaggatt ggatctcccc cgagcttggg atcgaagatg
      601 ccaatgcgga acagtcgctc aagccccacc agcacgatca cgatcgccac cacgggcgtt
      661 cccagaatct gaatcaggct cgtcgcataa tgcgcccaca cgaatagagg cagccggaac
      721 cacgtcaaac ccggcgcccg catcttatgg attgtgacga cgaaattcag accggtcaga
      781 atagacgaaa atcccgtaat gaacacacca agcgccgtcg cgattacgtg tgtgttcgag
      841 tacgtgctgc tgtacggagt gtaaaacgtc caaccggtat caacgccgcc tgccgccatc
      901 gcgtagagcg taaacagacc gccggcgatg tagatgtacc agctcagcag gttcagcctc
      961 gggaacgcga gatcccgcgc gccgatcata atcgggatca ggaagttacc aagtactgcg
     1021 ggaatggacg ggatgagaaa gaagaagatc atcatcaccc cgtgcatcgt aaaggccttg
     1081 ttgtaggtct cggcgcccat caggtcgccg gcgggcgtaa tcaactcgag acggatcagc
     1141 accgcaaacg caccgccaat gaaaaagaag aaggtgatcg cggcgaggta gagcagcgca
     1201 atgcgtttgt ggtccttggt caagagccat gacttgatgc cgtaaccggc gttcaagtag
     1261 ttcgtacccg cgtcagtact cggttctaca gtgctcgtgc tcattgctgc gtctcaaccc
     1321 tcttttttgg cgcgagggac ttgatatagg cgaccacctg cagtagcccc tcctcgctga
     1381 tctgtccctg gaaggtaggc atctgaattt tgtatccggc ggctactttc gcgcccggcc
     1441 tgagaaccga ctcgcgcaag taggcctcgt caaccacgac tttctgtcct gtctcaagca
     1501 gcaccgtgct cccgaatact ccttcgagcg ccggccccct tccactggcg tcctgcatgt
     1561 ggcatgtgtg gcatcccaga cgctggaaga gccgctcgcc agcctgcacc atcgtctcgc
     1621 cggtcgaagt cccgctcagc caattctcgt agtctgtcgg atccattacg tagacccacc
     1681 cgatcatccc cgaatgctgg ttgccgcagt attcggcgca gaagaggtgg tacttccccg
     1741 gcttgttggc ctggaaccac atcgaggtgt acatacccgg cacgacgtcc cgtttcaccc
     1801 ggaacgccgg cacgaagaaa ctgtggatca cgtcctcggt cgtcatcgtg agcttcacgg
     1861 gccggcccgc tggaatgtgc aactcgttga tctcgcggtg gccctccggg tgctggagtt
     1921 tccacatcca ctgcttgccg acaacgtaga tctccattgc gccctcgggc gggtggtaat
     1981 tgcggaagta cacgtaggcg ccccagccga acatgatcat cgcgatcatg cctggcgtga
     2041 ctgaccagaa gatctccagc agagtcgagc cgtggatcgg ccgcggaatc tcgtccggcc
     2101 ggcgccgccg gtatttgatg gcaaacacga aaattgtcgc gaagatgagc gtggagaaga
     2161 agacgcccac cgccaccagg aagaagtaga ggttgtccac tcccgcagcg atgctggatg
     2221 ccttctcggg aaatagctga aagtcggtcc acattgtgcg tatcaggtct tgcgccggtc
     2281 gcgccggaga ttcacgaaca tgaacagacc gagcaagaac accgtggcca tgccagccgc
     2341 gcgaagcgcg ttcatgatga caaacccgta cttgccggtc ttcggatcgt agtggtagca
     2401 gagcagtagc agctggtcga ccggggacag gatcctgccc gccgacgcct cgaccagcgc
     2461 gaaccgcaga tccttggccg aatactcgac cccgtacagg taccgcgcga tcttgccctg
     2521 aggggtgagc accatgattc cgctcgcgtg agcccactgg ttcgtttgcg catcccaggc
     2581 gtagcggaat ccgacggact gcgcgagctt cttaatggag tcttcatctc ccgtcaagaa
     2641 atgccagccc tccgaaccgc cctttcgccc gtactttttc acgtactccg ctttcttcgg
     2701 agccgccaat tccgggcctt ccttcggatc gaagcttacc gtgaccactt cgaagtgctc
     2761 gtgaacgttc agcggcgtgg cccgcaggct ccgcagcagc ccgttcagca ccatcgtgca
     2821 gagcatcggg cactcgtaat agaccaggga caaaaccact ggcttccggc cgaagtattg
     2881 gcgaagctgg acctgctttc cggtttcatc gcgaaacgtc aggtcgagcg gcgcctgctc
     2941 gttcaggcgc tggtcgatcc cgactttgct tagcggctcg ggcatgcccg tcgtctgagc
     3001 ctgagccgca acgccgagca gaatcccggc gaagacaaaa acgagcgcgc gtaacataaa
     3061 gtgcacctat ttcttctcct gagcctgcgc ccggaccggc agcccccgcc gcgcggtcag
     3121 ttcgatggcc cgctctatcg gaatcctcac cacgccggcc tgccgatcca cccagccata
     3181 actgttcagg gcggcctgcc cggcggcgtg caacttttcg atgtcggtgt tgggcgttac
     3241 ctgcaggcgt ggctccggcg gaaccgttcg ctccagggcg agcggcgaag gaaccggccc
     3301 gggttctttc cgctcggcga ggaaacggaa caacccgccc atcagtagaa gcgctaccgc
     3361 cacgagcgcg gtcagcccga tcccgaatat cacgacgccg cggatgttcg cgtcctgtgg
     3421 ttcgtagttc ttcacgccgg gcgccctcct tccaacggaa actcggcggc aggcagcaac
     3481 ggccgacgtt tcagttgcga caagaacacc gccagccaaa tcccgcccac acccaccggc
     3541 gctgcgaggt ccatccaatg cacttggatc cggttctcgt gaaacgccgg cgccacgacc
     3601 cagaacaagt cgaccagccg cagcacgagc atggcgcagg caagtatcgc cagaacaccg
     3661 gcccggcgtt tggcctcccg aaccagcagc agaaagaagg gcaagataaa gtggaatccg
     3721 atcaggattc ccgcaatcca gccccagccg ccgctgagac gccgtacata ccagggcgtc
     3781 tcctcgggca gattgcccga ccagatgatc aggaactgcg agaacgccac ataggcccac
     3841 agcatcacga atgccagcat caggttgccg agatcgtgga agtgcgaggg ccgcagcacg
     3901 cccttcatcg gctcccggcc cgacagcagc gtcagcgcaa cgatcacaaa ggcgagcgtc
     3961 gccaggacct gtccaacgat gaacaccgcg ccgtagatcg tcgagaacca gtggggttcg
     4021 agcgacatga cccagtccac cgccgcgaac gtcgccgtca gtccgtagag caccagcccc
     4081 ggaccgctca aatactggag gcggcttttc actgacgccg tgggcgtccg atcctgttct
     4141 gcggaccact tgttgaggaa gtacgagagg atcagccaaa ccgcaaaata gaatgccgag
     4201 cgcgccagaa agaacggcac gttcaggtat gggctcttgt gctggagcag ttcgtcatgg
     4261 gccacaacct ccggccgcgc ccagagatag aggtgcggta aaccgaacag cagcggcacc
     4321 agcagcaacg ccatcagcgg aaaggttctt gtgccggatt cgagcaggcg gcggatcatg
     4381 acaccccagc gtccgcccac caggtggtgg agcatgacaa ccgaaaagca gccgagcgcg
     4441 attccggccc agaacaggta tgccaccaga tacgagcgga agaactgcgc cgggttgaac
     4501 gcggcgccta gtgcgcacac ggccagtccc gcgccgccga atgccagcgc cagcttctga
     4561 gccctcttga agtcctccgt tcttgccatg ttcgcgttca tttcgcgcct tccatcaacc
     4621 gcttctgctc ggacgccggt gcgtcctcaa gcgtcgcgtt ctggctcagt tgcaacgcgc
     4681 gcacgtatgc cacgatcgcc caccggtcag ccgtcggaat tcgcgaggcg taactcgcca
     4741 tcgccccgaa accgttcgtc attacgtcga agtagtgacc aaccggagct tcccgaagcc
     4801 ggtcgctgtg aaacgatggc gggtggcgga gaccgcgctg cacgatcatc ccttcgccgt
     4861 cgccgagcag accgtggcac ggcgtgcagt agatgccgta acgctcgcgc ccacgttcca
     4921 gaacttcacg ggtcgcgggg aatggaagca ccgcggccgg atcgtttccc gttttgcccg
     4981 tgtagtacag ttcgtcctcc cggagttgtc cccgtgccac cgtgccttcc acctgcaaac
     5041 gcgatgccct gccatccgca aagaaactgc ttgccccgag cggcttataa cgcggctggt
     5101 cgtgcatatc ctgccggcag ccggcactga aaatcaccac agcaacgcag aaaacacaga
     5161 gcactcctga ttcctttgtg ttctttgtgc ctttgtggtt catctctcta aagctcaacg
     5221 tcagatacct cccggctgtg ctggctctcg aggaacttgc gggtggcctc gtaatcgaac
     5281 ttgggatcct tggcttcaat gcagaggaaa aacctgtcgt tgcttgccag ggcgaaactc
     5341 ggaacgttaa acacggggtg gtacggcatt ggcaatccgt tcagcgccag cattccgaac
     5401 actgctgcca gcgcagcgaa cagaacagtg gtctcgaacg tgatcggaat aaacgccggc
     5461 cagctattga aaggcttgcc gcctacgttc agcggataaa tgatcgtttc gatcgaccac
     5521 agcatcaggt acgccgcacc gcagccgaac agcccgccaa tcagcaccag caacggcagg
     5581 tagttgcgtc ggaagccgat cgcctccgcc agcccgtgga tcggcatcgg cgtgtaggcg
     5641 tccatcttgc ggtagccttc ggcgtacgcc cgctgcgcgg ccgcaagcag atcgcccggg
     5701 ctttcgaact gcgcgattac tccgtacagt cgccgtctct ccatacgctc agtgcttcgc
     5761 cttcccgtcc ggctcaggga cgagcgttct catctcaaaa atcgaaatca tcgggagcac
     5821 acggatgaag aggaacatca gcgccaggaa gaacccgatc gtcccgatgt acatgctcca
     5881 gtcgtacttc gtcccgtggt acatgcccca cgaggatggc aggaaatcgc ggtgcaggct
     5941 cacgacaata atcacgaacc gttcaagcca catcccgacg ccaaccacga ggctgatcag
     6001 ccagagcgca agcagattcc tgcgaaatcg gctgaaccac aacaactgcg gcgtcaggat
     6061 attgcagaag atgagcgccc agtagtaccc ctggtagggc ccgttcatgc ggttcgaaat
     6121 catgtactgt tcgtactggc tgccgctgta ccaggccatg aaggtttcca tcaggtagcc
     6181 gtagccgacg atcagcccgg tcgccagcag aatcttgccc atgttgtcaa tgtgccgcat
     6241 cgtgatgaag tcttcgaggc ggtagaactt tcggagcggg atcgccaggg tcagcaccat
     6301 cgcgaatccg gcgaagattg cgccggcgac gaagtacggc gggaacaccg tcgtgtgcca
     6361 ccccgggatg atgccgatcg cgaagtcgaa gctgaccact gtgtggaccg aaaccaccag
     6421 cggcgtggcg agccccgcca gcagcaggta cgccgtctcg tagcgctgcc agtggatcgc
     6481 cgacccccgc cagcccatcg acagcatgcc gtacaccacc cggagcggcc ggcttttcgt
     6541 acggtcccgt agcgtggcca agtcgggaat cagtcccaca aaccagaaca gcagggacac
     6601 cgtggcgtac gtcgacaccg cgaacacgtc ccacaccagc gggctacgcg gctgcggcca
     6661 cgtacccatg gtgttcggat acggaagcag ccagtagccc agccatggcc ggcccatgtg
     6721 catcagcggg tacaggcccg cgcacgccac cgcgaacagc gtcatcgcct cggcgaagcg
     6781 gttgatggag gtgcgccact cctggcgcag aagcagcagg atagccgaaa tcagcgtccc
     6841 ggcgtggccg attccgatcc accagacgaa gttgatgatg tcgaagcccc acccgacggg
     6901 aatgttgatg ccccacagtc cgactccctt caccagaagc gaggcgattg tgaacagcag
     6961 catcatcgtc agcaggaacg agatcgcgaa gccgatgatc cacccgcgcg gggtcttcgt
     7021 cgtcagcaca atctcgctga tcttgccggt gaccgacgcg tatgtgtgcc ccgggccgat
     7081 gacaggaacc tcacctgcgc gcgttgccgc gactgccgcc gcacccggat cttgacgctg
     7141 ttccgcttcc attgcgctag cctttcttca gctccgggtt ggggttccgc agtcgcgcca
     7201 ggtaggttgt tcgcggcttg gtgttcagct cgtccaggat gccgtagttc agcgggtcct
     7261 tcttcaactt cgccacgcgg ctgttcggat cgttcatgtc gccgaacacg atcgcctgag
     7321 tggggcacac cgcctggcat gcggtgacca cctcgccgtc acgaatcctg cggccttcct
     7381 tctccgattc aatccgcgcc gagttgatcc gctgcacgca gtaagtgcac ttctccatca
     7441 cgcctcggct gcgcaccgtg acgtccgggt tccgcatcgg ctcgaggctt ttcgtatccc
     7501 agtccgagta caagaagaag ttgaagcgcc gcaccttgta cgggcagttg ttcgaacagt
     7561 agcgggtgcc gacgcagcgg ttgtagacca tctggttcag cccctcgctg ctgtggttcg
     7621 tcgcgcctgt cgggcagacg acctcgcacg gcgcgtggtc gcaatggtgg cacatcatcg
     7681 gctggtggca cacctccgga tcgtccagcc cgcccttgaa gtagcggtcg atccggatcc
     7741 attgcatctc ccggccgcgc gccacctcgg tcttcccgac cacgggaatg ttattctccg
     7801 cctggcaggc caccacgcac gcattgcagc cggtgcatgc gttcaggtcc accgccatgc
     7861 cccacgaatg gccctcgtac ttgaattcct tgtagagcga caactccttc accgggccgt
     7921 gaaccacgtg ttgggcgaag tgcggatcct tgcggtactc gtcgacggtc gcctcgcgca
     7981 cgaggtcccg cccttccatg ctgtggtgat cctgcgtgca cgcgagcttg tacgtcgcgc
     8041 ccgtcttcga gaccgccagt ccccctccgc cccacggggc gtccgaagtt cgcagcggat
     8101 aagcgctgaa gcccggcccc tccgcgatct tgcctccccg ggtgcggccg tagccgagat
     8161 gaacggtcgc cgagttttct gcgtggcccg gaaggatcca cgccgcccca cggaccgtcc
     8221 ggccggcacg tcgcagctcc aatacctgcc cgttggccac gcccaggcgc tcggcagtcg
     8281 ccgggctgat gagcactgtg ttatcccacg tcagcttggt cagtggcttc ggcaactcct
     8341 ggagccaccc gttgttcgcg aagcggccgt cccaaatcgt cgggtcagga cgcaggcaca
     8401 attccattcc ttcctggccc ttcggcgccg gcagagcggc cgcgaagtcc tttcgcaccg
     8461 aaacgttctt cggggcgacg gccgtgccgg ctatcaggcc gtcgtgcagc gcagtcttcc
     8521 acgttttctc gaaccccgcc tcgccgagcc ggccacgcca gtactctttg atgatgtcgt
     8581 gtcccgccag ttcgcccttg cccgacagca cggcgagtac ttcgtgcgcc gacttgcctc
     8641 cgtataacgg agcgatcagc ggctgctgga tggttaccgt cccgtcaaac gccctggcat
     8701 caccccagcc ctcgagatag tgggtctcag ccaggtgcca gtggcacagg ccggatgtct
     8761 catcgtcgta caaccccaac cggatccggc gcttcacctt gagcagcccc tgccggaact
     8821 cgcaatcggc cggcgcgttg tatacgggat tgccgccgat gatcacgagc gtttcgaccc
     8881 ggcctgccct catgtcctcg gcaagctgcc gcatggattc gccctgctcc acgggcgccg
     8941 cttcaaccgg ctccgtatag acgacggtct tcccggcgtt gccgagagcc gcgttgatgg
     9001 cgtgcgcgag ggcgtggact gccactggct gctggtcgcc cgcgattacc aggctcgacc
     9061 cggcatgctt ctttaggtcc gccgccaccg cctccaccca tccggacggt gccggcgagc
     9121 ccggtgccgg cacagcgact cccagcgccg ccgccaccgc ccacgcgaat ggttccacgt
     9181 cgcttgcacg caacggcaat cgatggtcgg cgatgcttcc cgtgctcgac ggggccgctt
     9241 caacgatgta caggcggttc atccggtcgc cgccttcccg gcgccgcgcc gcaaactccc
     9301 gcgcataacg gacgctgccc ggaccggagg ccaggaaatc cgcgtcgagc gaaagaatca
     9361 cgtcggcctg gccaattctg tggattgtgt tcaccggttc gccgaaagcg agcttcgcgc
     9421 cgccgaccgc gctgtccctc gccgccggtt cgtattggtg ccaccttgct tccggaaact
     9481 ccgccagcag gtccttcaac tgctgcgcta gtgtgggcga cgtcacggtc tcagtgagga
     9541 tgcgcatccc ggcgccctct ttcgcgagca ggccttcgcg caacaggccc acttccgcca
     9601 ggaacgcggc ccagttgctg ataagcccgt tccgcaacac cacttgcgac cggtccggat
     9661 catagagcgt caggatcgac gcctgcgcga aggcatcggt cgcgcctaag ctcgccggat
     9721 gctccggatt gccctcgatc ttcgtcggcc ggcccatgtg actctccact aaaacgccgg
     9781 cggcgtaccc gcccagagtc atcgccgtag cgaagtacat cggcttgccc gggatcatgt
     9841 cctcgggagc tcggacgtag gggacgatct tttccggcgg ctgcttggtg caggcggtga
     9901 ggcccgcaaa ggcgatcgac gctcccagca gcttcagcag gtcgcggcgg ccgagcgggt
     9961 tcttccattc ggaagtgccc ggcgtgaact cgttgtcgac aaacgcctga aattcggcgg
    10021 tctgggcgac ttcgtccagg ctgcgccagt actcctctcc ccgggcgttc tccagacgcg
    10081 cgcggatcgc ggacaggtcg agcagtgaat tcttcggttg catcatcggt ggcagacgga
    10141 acagtccgtc aacttgcgga tcttgtattc ctcgacgagc ttgcgcccca aggcgagctg
    10201 gtcagaaggc gcccggtagg ccatgttgaa aacctggccc cgagggcgca cgaagcgctc
    10261 cggctgccgg tgacactcca ggcaccactc catgtaaagc gtgttcgtct tccacatcag
    10321 cggcatctgg tcaacccgcc cgtggcaggt cgcacagcca atcccctttt tcacgtgaat
    10381 gctgtggttg aagtacacaa atcccggcag atcatgcacc cgcgtccagc ggatggactg
    10441 gtcgttccga aagcttgccc gcaccggttc gagcatcggg ctatcggccc agatctgcga
    10501 gtggcagctc atgcacgtct cagtcggagg catcccagcc gaagaggaaa tctcgaccgt
    10561 cgagtggcag taccggcagt cgatgccaag cccggccaca tggtgtttat ggctgaacgg
    10621 caccggctga tcgagcgcga ctccaacgtc ggtaacgtac ggcgagcgat tgacctcgtt
    10681 gaggacccag agcaacccgc cgccgatgaa cagcgccccg aagatgctca cccgggcgat
    10741 cgtattcgta cttggatgaa agatctgcaa gcccagtacc caaacatccc ctcaggcaga
    10801 cccagacgcc ccgccttcgt aggtaaatgt taaacatcca gtatattttc ttgtcatacc
    10861 gtatgtcaaa gccctataat ccccgcaaat aaaagaaaaa aggaggttta cctctgttaa
    10921 caacagccgg tgggaaaggg gtttcacggc gtttcagggg ggtgacgatg acttggttct
    10981 tgtgcggccg accggccgcg tggcaggact accctcacgt gaagcgactt ttgccaccgc
    11041 cgccgcaccc gccagtcgcg aaggccgaga gcagccgctc gatcgcttcc gactcgcagt
    11101 ggggacactg cattacgcgg tctgcgtccg ccatccggcg tatcttctca aacgtccgcc
    11161 cgcagttccc acaccggtat tcgtagagag gcatgctgtc atccatgatg caggggaccg
    11221 cggtgaggtg acagaccctc tgccacttct ccgaaagctg ctgtaatctg gggaatctat
    11281 gcggagaatc ggccttctcg ctctgtttct tgccgctttg gtgcccgccg cccaggacaa
    11341 attcgaatac tggccgggcg ccgtctacga tccggcgatc cccacgttcc gaaaagtgct
    11401 cggctacgag accggcgagc gcatctcgtg gcacgcaaat ctcgtgaagt acctcgaagc
    11461 attggcggcc gccgcgcccg accgtgtcaa ggtgttcgag tacggcaaaa cctgggaagg
    11521 ccgccggctt gtctgtgccg ctatcagtgc gaaggagaat atccggcggc tcgatcagat
    11581 ccgctccgcg atgcaaaagc tcgccgaccc caggcgcact cctgaagcag aagcaggcaa
    11641 actgatggcg ggtcttccct ccgtcatctg gctcgcctac ggcgtccatg gcaacgaact
    11701 ctcgtcgcca gaagcctcgc tgctcaccgc ctatcacctg ctggctgcgc gaaacgacaa
    11761 gatggtccag gacattctcg ccaacgttgt ggtcttgatt gacccagacc agaaccccga
    11821 cgggcacgac cgcttcgttc acaacttcga gcagaatgag ggaatcgagc ctgacccgag
    11881 cccgctcgcg gctgaacaca gcgaaccgtg gccaagcggc cgggtcaatc actacttctt
    11941 cgatctcaat cgcgactggt tcgcgctcac acagtccgag acccgtggcc gggtcaggtt
    12001 gatgctcgag tggttcccgc aagtctgcgt ggacctccac gaaatgggcg ccgactccac
    12061 gtactacttc gcgcccggcg cgcttccctt caacccgcat ctgacgaagt tccagcggga
    12121 gggtttcgac atcttcggca agaacaacgc gcgctggttc gatcagtttg ggttcagcta
    12181 cttcatccgc gaggtcttcg acgagttttt cccgggctac ggaacaagct ggccctcgct
    12241 ccacggggcg atcgcaatga cgtacgagca agcttcctcg cgcgggctcc tgatgcgccg
    12301 gaccgacgag atgctgctgc gcttccgcga caccgtccgc aaccactttg tcgcgtcact
    12361 ctcgactgca gaggcttcgg cgcggaaccg ggaaaagctg ctgggtgagt tttaccagta
    12421 tcggagcacc gcgattcaag aaggctccac cgaaccggtt cgcgagtaca tcctgccggc
    12481 gcgaggcgat aaatcggccg tggacaagct ggcggctttg atggtctctc tgggagctga
    12541 ggtgaagcgt gcgtccgcgc cgttccggaa cggcgacaag gaatttcctt
//
LOCUS       DATIFQ010000136        13952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1002097, whole genome shotgun sequence.
ACCESSION   DATIFQ010000136 DATIFQ010000000
VERSION     DATIFQ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13952
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002097"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VLE22_06155"
     CDS             <1..258
                     /locus_tag="VLE22_06155"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HSB14021.1"
                     /translation="AWTDPSETDVNIRWAREFWTAMQPFLADAVYVNYLGEVEEERVR
                     AAYGRKYDRLASLKAKYDPTNFFRMNQNIRPARSASETAAH"
     gene            650..1147
                     /locus_tag="VLE22_06160"
     CDS             650..1147
                     /locus_tag="VLE22_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB14022.1"
                     /translation="MTLRIEKLQRGHVLEGFDCGREALNRFLIRYALQSQQAGASQTY
                     VALANAEVAGYYTLVVGQVEYDGAPERLKRGLARHPVPIMLLARLAIAIGWQGKGLGS
                     GLLKDAMLRTLQAADIAGIRAFVVHAKDAEAKSFYERYDFTASPSDPYHLFRLLKDIR
                     AALQF"
     gene            complement(1192..2181)
                     /locus_tag="VLE22_06165"
     CDS             complement(1192..2181)
                     /locus_tag="VLE22_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSB14023.1"
                     /translation="MAVVLPPLATIFLLWQGCARRPENQRAGRFPEELVYVRSEDDIV
                     NGGAIFVPPKESAKPIAVVWIHGWGVNFYYPTYVGIGRALAERGYACISGNTRMHDLA
                     NVAAWRGEKRIRGGGYWGVASEEVRDLAAWIDFAEDRGFKKVVLVGHSAGWSAVRRYQ
                     AEKQDRRVAGVVLASGAVQADTRVPDPEQLAEATRLMAEGKGDDLVRDPKRSFPSFTS
                     AATFLDIANAPPEFKDFFGVQTPNPAVTRIRCPLLAFFGTNGDVGTQQDLELLKSSIQ
                     RQSSGPSRVNTVMIQNADHMYTGEEAQVAQTIAGWADTLLSPEAGKAEVPGRR"
     gene            2391..3494
                     /locus_tag="VLE22_06170"
     CDS             2391..3494
                     /locus_tag="VLE22_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB14024.1"
                     /translation="MHGNGSTGKNRPRLVFWELTTGCNLRCVHCRASATELMSPDDLN
                     YQECCDIVDQIAEYAPLILVLSGGEPLWRRDVFDIAKRATGKGIRVALATNGTLVDEA
                     MAQRIEESGILRVAISLDGADRITHDTFRGQSGAFDSAIAGLKHLSALGISTQINTSV
                     ARHNVHQLPDILKLAQSLGVDAFHIFLLVPVGCGLTIAEEQSIRGEEYEQILNWFYDR
                     SIDSGLELKATCAPHYYRIMRQRRAEARRAGMAVPELASHGMHAVTRGCLAGSGVCFI
                     SHEGKVYPCGYLPVEAGNLRRQPFREVWESAQVFLDLRDPDRLEGKCGICEFKQVCLG
                     CRARAFGMMGDYLAEEPFCVYQPRGTEGPERHV"
     gene            3566..4075
                     /locus_tag="VLE22_06175"
     CDS             3566..4075
                     /locus_tag="VLE22_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HSB14025.1"
                     /translation="MPLLAERFTAQAAPPRDNREKLLERAMSRHRYSGDSLIEVLHTA
                     QELYGFLTPELLTKIARKLRLPPSRVLGVTTFYHFFSLAPKGEHSFIVCTGTACYVAG
                     AQGLLETLERRCHCKSGGTSPDGKATIAAARCIGSCGLAPAVIYDGNIMARVKPDQLN
                     AELDRIGVQ"
     gene            4072..5727
                     /gene="nuoF"
                     /locus_tag="VLE22_06180"
     CDS             4072..5727
                     /gene="nuoF"
                     /locus_tag="VLE22_06180"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HSB14026.1"
                     /translation="MTVEELEQKQADEQERQRGLENRIYCCTAAGCVSSGADRLKQAL
                     TDEVKAQGLDGKVEVCGTGCMGLCSRGPLVRSSKDDALYGNIEPAEAAALVRGAAEDQ
                     EKLAPFRIDTTAPFFAKQEKIVLANSGFVDPEKLPDYIAAGGYGALARAITEMSPQEV
                     LEEIKASGLRGRGGAGYPTGLKWELVARQPAQPKYVVCNADEGDPGAFMDRSVLEGDP
                     HRVLEGMAIAGYATGAQQGYIYVRGEYGLAILRLQAAIKQAEREKLLGNRVLETGFQF
                     RVDIRIGAGAFVCGEETALIASIEGKRGQPRQRPPYPPESGLWGNPTLINNVETFGNV
                     PAIITKGSAWFSAIGTPASKGTKVFALAGKINNTGLIEVPMGTTLRTILFDIGGGVPE
                     GGEFKAAQTGGPSGGCIPAEFLDTPVDYESLAKIGSIMGSGGLIVMDDTSCLVDVARF
                     FMEFCADESCGKCIPCRAGTVQMHDILHRITRGDCHEKDLKILEELAKLLREASLCGL
                     GQTAPNPVMSTLRFFREEYEAHIRDHKCPAGHCSFDGKDGGGR"
     gene            5724..6440
                     /gene="hoxU"
                     /locus_tag="VLE22_06185"
     CDS             5724..6440
                     /gene="hoxU"
                     /locus_tag="VLE22_06185"
                     /inference="COORDINATES: protein motif:HMM:NF005745.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HSB14027.1"
                     /translation="MSVKTLQIDGKMVTGLAGQTIFSAAWDNGIQIPRLCHVGGLTDV
                     GACRLCLVEIEGQRKLQAACMTPIEEGMSVRTDNDRLRAHRKLIVELLFAERNHVCSV
                     CVMNGNCELQELAASLGIDHIRLRYQFPALSVDTSHERFGIDHNRCVLCTRCVRICDE
                     VEGAHTWDVSGRGTHSQIISDLGQPWGDSDTCTSCGKCVQVCPTGALFEKGKTVSEMR
                     KSRTFLKYIVTAREKGEWIK"
     gene            6437..6970
                     /locus_tag="VLE22_06190"
     CDS             6437..6970
                     /locus_tag="VLE22_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14028.1"
                     /translation="MKDKPRLATVWLGGCSGCHMSFLDLDERLAELAGRVEFLASPLT
                     DFKEFQDADITLVEGSVANVEHLEQIREIRKRTKILVSFGDCAVTGTVTAMRNAFPVE
                     EVIDRSYVENAAVVIGRPGGGDVVPALLDRVRPVHEVVEVDYFLQGCPPSADDIYGFV
                     MDLLNGRTPRTDGRRFG"
     gene            6971..8395
                     /locus_tag="VLE22_06195"
     CDS             6971..8395
                     /locus_tag="VLE22_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HSB14029.1"
                     /translation="MAERILIAPVTRIEGHAKISIFVNDAQEVESAMFHVTEFRGFEK
                     FCEGRPFHEMPGLTARTCGICPISHILASSKACDALLGVEIPPTAVLQRKLMNYAQIL
                     QSHALSFFHLSSPDFLLGFDAPIEKRNIFGLLAEDPDFARRGIRLRKFGQRIIELLGG
                     KRIHPAWGTPGGVIGTVDPSERAEIATGVPEALESVKLALGRMKTIIDSCPDEVEHIG
                     NFPTLHLGTVTPTGELEYYDGVIRIVDGEGNIVADGLDPQRFHTYLGEASEESSYMKF
                     PFYKPLGYPGGMYRVGPLARLNIARAAGTALADRELREFKHRSTGAVNQSFHYHLARL
                     IEMLHCVERIEEIASDPVLLKTRVRAVAGQNRNEGVGSSEAPRGTLFHHYEVDDNGLI
                     RKVNLLIATGQNNLAMNKTVEQTAIRFVNPRALREEMLNRVEAGIRCFDPCLSCSTHA
                     AGQMPLMLEIRNRRGEVLGRLARR"
     gene            8392..8805
                     /locus_tag="VLE22_06200"
     CDS             8392..8805
                     /locus_tag="VLE22_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HSB14030.1"
                     /translation="MTFLIAVGNTLRRDDGVAHRVLELLKPPAGVVVRSCHQLTPEMA
                     EEIASADTVIIIDADVAPGEPRLEKVEERPDNPLTHVVRPAELVALSRRLFSFRGEAW
                     LCRVPGIDFGQGSGLSPATEANACITAELLLRFLG"
     gene            8972..9748
                     /locus_tag="VLE22_06205"
     CDS             8972..9748
                     /locus_tag="VLE22_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14031.1"
                     /translation="MKPNYRLLVVLFVVAALAVAWAQGPRGRAQSAPGNGQGQPALDM
                     TKLVTVEGPVSAIQLNYGAQYPTIEVNKTLIKIAPVWFLIENDFEIDVNDVVKVTAAL
                     STADAYLHAVEIVNVTNSNALITLRDKSGTPLWTTRQAGRAGRWSQDRMLRGNGGGQA
                     CLDMASGTTVSGIVSQVTMGTGIKQPVVVIEQADKSLISVKIGPERILLENDVELTAG
                     EAVTAKIAKSACKDEWVALELTVDGKTVALRTELGAPAWN"
     gene            9838..10224
                     /gene="crcB"
                     /locus_tag="VLE22_06210"
     CDS             9838..10224
                     /gene="crcB"
                     /locus_tag="VLE22_06210"
                     /inference="COORDINATES: protein motif:HMM:NF010802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HSB14032.1"
                     /translation="MIYLWIALGSAIGGAARYWSYGISARLFGETFPWGTLAVNVIGS
                     AFIGFFATLTSPDGRLLVPSSTRQFVMAGVCGGFTTFSTFSLETLNLARDGEWGRVLG
                     NVLGTVILCLAGVWCGYALATALNER"
     gene            10234..10572
                     /locus_tag="VLE22_06215"
     CDS             10234..10572
                     /locus_tag="VLE22_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HSB14033.1"
                     /translation="MQIPRDAVLLRIFLGENDRHGHQPLYEAIVLKAREMHLGGATVL
                     RGPMGFGHSSRLHTAKILRLSEDLPIVVEIVDSEAKIDAFLPILDTMIGSGLVTLEKV
                     TVLQYGKAGK"
     gene            10905..11279
                     /locus_tag="VLE22_06220"
     CDS             10905..11279
                     /locus_tag="VLE22_06220"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HSB14034.1"
                     /translation="MSSSAVEVQEARAQSVKVTDDALSVDLVDGRTIIVPLVWFPRLW
                     HGTAQERYRFEIFGDGAYIHWPALDEDLTVSGLLAGRQSGESPESLRRWLTSRKIRRK
                     PRASKELSRQSAAPPRKAARRG"
     gene            complement(11690..12577)
                     /locus_tag="VLE22_06225"
     CDS             complement(11690..12577)
                     /locus_tag="VLE22_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14035.1"
                     /translation="MRLTFLIPALLLLVLPPPAHACTAFCAAAKGRVLVGNNEDLFDP
                     HTRLWFFPAENGEHGRMYVGYDYAWPQGGMNDRGLFFDGFGVPLVKLPPSTKPPLQPV
                     ANRVPNRPFPGGALDRFLAECASIEDVIGLFRKYEFPWAEKSVLMFVDASGDAAIFDG
                     NTILRKKGRFLVQTNFRQSATRPGEAPCERFRIATRMLKSAGDNTNIDLFRRILAATH
                     QEGNNPTQYSNIYDLKRRIMYLYHFHNYENVVTIELSRELRKGAHSVAIPSLFPRTHA
                     AEKWAAEHQDALKKLLIKK"
     gene            12777..13730
                     /locus_tag="VLE22_06230"
     CDS             12777..13730
                     /locus_tag="VLE22_06230"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HSB14036.1"
                     /translation="MSESPQQDRLNQDLGLGGKMSERSRARLLNRDGTFNVRRNNLSS
                     FHPYNAYNTLLSLPVPRLLALVAAGYVATNLLFASLYWLAGPDALSGAAPSPLTRFED
                     CFFFSVQTLATIGYGKLTPHTRAANFLVAIEALVGLLGFAILSALLFARFARPTAKIT
                     FSRNALIAPYENGWALIFRLASLRNHELTNVHAIVSFARWIEVNGLRRRRFDQLPLER
                     DFIIFMPLHWAVVHPITETSPMRGFTPESLAAADPEIVCLINADDETFAQTVHGKTSY
                     DKSDIVWGARFRDMYLPDVDKVAIDLNRLHDFERVAPPSSL"
     gene            complement(13739..>13952)
                     /locus_tag="VLE22_06235"
     CDS             complement(13739..>13952)
                     /locus_tag="VLE22_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSB14037.1"
                     /translation="AAGDVSALTARELEVLRMMAEGAANKIIAWKLGISEHTVKFHVA
                     SILAKLGASSRTEAVTIGIRKGLILL"
BASE COUNT         2646 a         4463 c         4153 g         2690 t
ORIGIN      
        1 gcctggaccg atccatcaga aaccgatgtc aacatccgct gggcacgtga attctggacg
       61 gcgatgcaac ctttcctcgc ggatgcggtg tacgtcaact acctcggcga ggttgaggag
      121 gaacgcgtgc gggcagccta cggccggaaa tatgaccggc tggcttcctt gaaagccaaa
      181 tacgacccga caaacttctt ccgcatgaac caaaacatca ggccggccag atccgcgtcc
      241 gagacggcgg cccactgaac cgggaaccag agctctgatc ggacaagtgg aacagcccgt
      301 ccgctgtcat gacattgtct ggcctacgtg ttatcctgat cgtgtgagag taggaaagag
      361 ccatgccggc ttcggaaacc cgtaaggaga aacttgatct gcgcctgact ccgtctgcga
      421 agcgcaccct tcaagttgcg tcccaggctg ccggccggtc agtcagcgag ttcgtattgg
      481 aaagcgcgct tgctcgcgcc gaggaaacct tgccggatcg ccggcgtttt ggtttgagcg
      541 ccgagcagtg ggccgctttc caagctctct tggatgctcc tcctccgccc gctcggcgac
      601 tggccaggct cctccgtcag ccgagcgtct tcgagcgcgg ccccaggaaa tgactcttcg
      661 cattgagaag cttcagcgcg gacacgtgct ggaaggcttc gactgcgggc gtgaagcgct
      721 caaccgcttt ctgatccgct acgcgctcca gagccaacag gccggagcgt cccagacgta
      781 tgtcgcgttg gcgaatgcag aggtcgccgg ttattacacg ctggtggtcg gtcaagtcga
      841 gtatgacggc gcgccggagc gtctgaagag aggtctcgcc cgccatcccg tgccgattat
      901 gctcttggct cgtctcgcga ttgctatcgg ctggcagggc aagggccttg gttccggtct
      961 cctgaaagat gcaatgcttc gtacattgca ggcggccgac atcgccggca tccgggcttt
     1021 tgtggtccac gcgaaggacg ccgaggcgaa gtcgttctat gaacgctacg acttcacggc
     1081 gtcgccgagc gatccgtacc atctatttcg cctactcaaa gacatccgcg ccgcgctcca
     1141 gttctgattc cagtttgtgg ttcagccgga atggagggat ggcgtcttct gttagcgtct
     1201 ccctggaacc tcagctttgc ctgcttccgg tgacaacagg gtgtcagccc acccggcgat
     1261 ggtttgggcc acctgtgcct cttcccctgt gtacatatgg tcggcgttct ggatcatgac
     1321 ggtgttcacg cgactggggc cgctcgactg acgctggatg ctggacttaa gcaattccag
     1381 gtcctgctga gttccgacat ccccattcgt gccgaagaag gcgaggagcg gacagcggat
     1441 acgcgtgacg gccgggtttg gtgtctgtac tccgaagaaa tctttgaatt ccggaggcgc
     1501 gttggcgata tccagaaacg tggccgcgct ggtgaaggac ggaaaggagc gtttgggatc
     1561 gcggaccaag tcgtcccctt tcccttcagc catcaaccga gtcgcttctg caagctgttc
     1621 cgggtctggc accctcgtgt ctgcctgaac cgctccggag gcaagaacca cccccgctac
     1681 gcgtcggtct tgcttttcag cttggtatct gcgaaccgcc gaccagccgg cactgtgccc
     1741 taccagcacc accttcttga accctcgatc ctcggcgaag tcaatccagg cggcgaggtc
     1801 tcgcacctcc tcactagcca caccccagta accaccgccg cggatccgtt tctcccctcg
     1861 ccacgcggca acgttggcga ggtcatgcat acgcgtattc cccgagatgc acgcatagcc
     1921 gcgctccgcg agtgcccggc cgatccccac gtatgtcggg tagtagaaat tcacacccca
     1981 tccgtggatc cagacgaccg cgatgggctt ggctgattct ttcggcggta caaagatcgc
     2041 gccgccattg acgatatcgt cctcggaccg gacatagaca agttcttcgg ggaaccttcc
     2101 agccctctgg ttttcggggc gccgtgcgca cccttgccaa agcaggaaga ttgtcgccag
     2161 cggagggagc accacggcca tcgagataag tcgacaccgc cctgtcgaat cgtgtcgttt
     2221 cactagcctt ctgcctctct gggattattc ttccttgttt cttcaggacc gtccatgctg
     2281 cgaagtatat ccgaacacac gatcacaagc gcatcctggc ctgtgtcgaa gattaacgat
     2341 cctgcatcat tctctgcgtt ttctggccca tcaattgcac ccggaacagt atgcacggca
     2401 atggctcgac tgggaaaaac cgcccccggc tggtgttctg ggaactcaca actggttgta
     2461 atctaaggtg tgttcactgc cgcgccagcg ccacggagct catgtctccg gacgacctca
     2521 actaccagga atgctgcgat atcgtagacc aaattgcgga atatgcccca ctgatcctcg
     2581 tcctcagcgg cggtgagccg ctctggcggc gcgatgtctt cgatattgca aagcgcgcga
     2641 ccggcaaggg catccgcgtg gcgctcgcca ccaacgggac cctggtcgat gaggcaatgg
     2701 cccagcgcat cgaggaatcc ggcatcctcc gggtggcaat cagcctggat ggcgccgacc
     2761 ggatcactca cgacaccttc cgaggccagt ccggtgcgtt cgactccgcc atcgcgggtc
     2821 tcaagcacct gagcgcactc ggcatctcca cccagatcaa cacgagcgtc gcccgccaca
     2881 atgtccatca gttgcccgac attctgaaac tcgcccagtc gctcggggtc gacgcattcc
     2941 acatcttcct gcttgttccc gtaggatgcg ggctgaccat cgcagaggaa cagtcgatcc
     3001 gcggcgaaga atacgagcag attctcaact ggttctatga ccgctccatc gacagcggtc
     3061 tcgaactcaa ggcgacctgc gccccgcact actaccggat catgcggcag cggcgcgccg
     3121 aagcccgccg cgccgggatg gcagtcccgg agctcgccag tcacggcatg cacgccgtca
     3181 cgcgagggtg ccttgcgggg agcggtgttt gcttcatctc ccacgaaggc aaggtgtacc
     3241 cgtgcgggta cctgcccgtc gaggcgggca accttcgtcg gcagcccttc cgggaagtct
     3301 gggagagcgc tcaggtgttc ctcgatctcc gtgatcccga ccgcctcgag gggaaatgcg
     3361 gcatctgcga gttcaaacag gtgtgcctcg gttgccgcgc ccgggcgttc ggaatgatgg
     3421 gagactacct ggccgaagag cctttctgcg tgtatcaacc ccgaggcacg gaaggaccag
     3481 agcggcacgt gtagcccggc tgacacggag cggaccgggg ctaaaggcgg acggttcgcc
     3541 atataacagg ttttaaggtc ttattatgcc tttattggcc gagcgtttca cggcgcaagc
     3601 tgccccgccc cgggacaacc gggagaagct gctggagcgg gcgatgtccc gccaccggta
     3661 ttccggggat tccctgattg aagttcttca taccgcgcag gagttgtacg gcttcctgac
     3721 gccggagctg ctgacgaaaa tcgcccgaaa gcttcgtctc ccgccaagcc gcgtacttgg
     3781 cgttaccact ttctaccact tcttctcgct cgcacccaaa ggtgagcact ctttcatcgt
     3841 gtgcaccgga acggcgtgct atgtggccgg cgcgcagggg ctgttggaga ccctcgagcg
     3901 gcgctgccac tgcaagtcag gcggaaccag cccggacggt aaggccacca tcgccgcggc
     3961 gcgctgcatc gggtcgtgcg gcttagcgcc ggcggtgatc tacgacggca acatcatggc
     4021 ccgtgtgaag ccggaccagc tcaacgcgga gctagatcgg atcggtgtcc aatgacagtc
     4081 gaagaactgg agcaaaaaca agctgacgaa caggagcgcc agcgcgggct cgaaaatcgc
     4141 atttactgct gcaccgccgc cggctgcgtc tcctccggtg cggaccggct gaaacaggcg
     4201 ctcacggacg aagtaaaagc gcaggggctc gacggcaagg tcgaagtctg cggcaccggc
     4261 tgcatgggac tctgcagccg cggaccgctg gttcgctcat ccaaggacga cgccctctac
     4321 ggaaacatcg agccggccga agccgccgcg cttgtgcggg gcgctgccga ggatcaagag
     4381 aagctggcac ccttccgcat cgacaccacc gcgccattct tcgcgaagca ggagaagatc
     4441 gtcctcgcca acagcggctt cgtagacccc gagaaactgc ccgactatat tgcagccggc
     4501 ggctacggag cgctcgctcg tgccatcacc gagatgagtc cccaggaggt cctcgaagag
     4561 atcaaggcct ccggacttcg cggccgtggc ggcgccggct acccaaccgg cctgaagtgg
     4621 gaactcgttg cacggcagcc cgcccagccc aagtacgttg tctgcaacgc ggatgagggt
     4681 gacccgggcg cgttcatgga ccgcagcgtt ctcgaaggcg atccccatcg tgtgctcgaa
     4741 ggaatggcca tcgcgggcta cgcgaccggc gcacagcagg gttacatcta cgttcgcggc
     4801 gagtacgggc tcgccatcct gcggcttcag gccgccatca agcaagccga gcgcgagaaa
     4861 ctcctcggca accgtgtgct cgaaaccggc ttccaattcc gcgtcgacat ccgcattggc
     4921 gcgggcgcct tcgtctgcgg cgaggagact gcgctgatcg cctcgatcga aggcaagcgt
     4981 ggccagccga ggcagaggcc gccctatccg ccggagtcgg gcctgtgggg caatcccacg
     5041 ctgatcaaca acgtcgagac cttcggcaac gtgcccgcga tcatcaccaa aggttccgcc
     5101 tggttttccg cgatcggcac ccctgccagc aagggaacca aggtctttgc gctcgctgga
     5161 aagatcaaca acaccggcct gatcgaggtg ccgatgggca ccaccctgcg cacaatcctc
     5221 ttcgatatcg gcggcggcgt tccggagggc ggcgagttca aggccgcgca gaccggcggt
     5281 ccgtccggcg gctgcatccc ggctgagttc ctcgacacgc cggtcgacta cgagtcgctg
     5341 gcaaagatcg gctccatcat gggatcgggc ggcttgatcg tgatggacga cacctcctgc
     5401 ctggtcgacg tcgcccgctt ctttatggag ttttgcgccg atgagtcctg cggcaagtgc
     5461 attccctgcc gggcgggcac ggtccagatg cacgacattc tccaccgcat cacccgcggc
     5521 gactgccacg aaaaggatct caagattctg gaggaactgg cgaagctgct tagggaagcc
     5581 agcctctgcg gcctcggcca gacggccccc aacccggtca tgagcacact ccggttcttc
     5641 cgcgaggaat acgaagccca catccgcgac cacaaatgcc ccgctggcca ctgctcgttc
     5701 gacggcaagg acggaggtgg acggtgagcg tcaagacact gcaaattgac gggaagatgg
     5761 tgaccggcct ggccggccag accatcttca gcgctgcctg ggacaacggg atccagatcc
     5821 cgcgcctctg ccatgtcggc ggtctgaccg acgtcggcgc ctgccgtctc tgcctagtgg
     5881 aaatcgaagg ccagcggaag ttgcaggcgg cctgcatgac ccccatcgag gaaggcatgt
     5941 cggtgcgcac cgacaacgac cggctccgcg cgcaccggaa actgatcgtc gaactcctgt
     6001 tcgccgagcg caatcacgtc tgttcggtgt gtgtgatgaa cggcaattgc gagcttcagg
     6061 agttggctgc cagcctcgga atcgaccaca tccggctgcg ctaccagttc ccggcccttt
     6121 cagtcgatac gagccacgaa cgtttcggga ttgaccacaa ccgatgtgtc ctctgcacgc
     6181 gctgcgttcg catctgcgac gaggtggaag gggcgcacac ctgggacgtc tcggggcgcg
     6241 gaacccattc gcagatcatc tccgacctcg gccagccgtg gggcgattcc gacacctgca
     6301 cgagctgcgg caagtgcgtg caggtctgcc cgaccggcgc gctcttcgag aagggaaaga
     6361 cggtcagcga gatgcgcaag agccgcacct tcctcaagta catcgtgacc gcccgtgaga
     6421 aaggggagtg gatcaaatga aggacaaacc tcgtctggcg accgtctggc ttgggggttg
     6481 ttccggctgc cacatgtcgt ttctggacct cgacgaacgg cttgcggagc tggccggccg
     6541 ggtcgagttc ctcgcgtcgc cgctgaccga tttcaaggag tttcaggatg cggacatcac
     6601 cctcgtcgag ggctcggtag ccaatgtcga gcacctggag cagatccgcg aaatccgcaa
     6661 gcgtacaaag atcctcgttt ccttcggcga ctgcgccgtc accggcaccg taaccgccat
     6721 gcgtaacgcc ttccccgtcg aagaagtgat cgaccgaagt tacgtggaga acgccgccgt
     6781 ggtgatcgga cggccgggtg gcggcgatgt cgtcccggct ctgctcgacc gcgtgcggcc
     6841 cgtccacgaa gtcgttgaag tggactactt cctccagggc tgtccgccgt ccgccgacga
     6901 tatctacggc ttcgtgatgg atctgctcaa cggacgaacg ccccgcaccg acggtaggag
     6961 gtttggataa atggccgaac gaattctcat cgcgccggtg acccgcatcg agggtcacgc
     7021 gaaaatctcg atcttcgtga acgacgccca ggaagtggaa tcggcgatgt tccacgtcac
     7081 cgagtttcgc ggcttcgaga agttctgcga gggccggccg ttccatgaga tgcccggcct
     7141 tacggcccgc acctgcggca tctgcccgat cagccacatc ctggccagct ccaaagcctg
     7201 cgacgcgctg ctcggcgtcg agattccgcc cacggcggtg ttgcagcgaa agctgatgaa
     7261 ctacgcccag atcctccagt cgcacgctct gagcttcttc cacctctcct ctcccgattt
     7321 tctgctcggc ttcgacgccc ccatcgagaa gcgaaacatc ttcgggcttc tcgccgagga
     7381 cccggacttc gcccgccgcg gcatccgtct tcggaaattc ggacagcgga tcatcgagtt
     7441 gctgggcggc aagcgaattc atccggcatg gggcacgccg ggaggagtca tcggaacggt
     7501 cgatccgtcc gaacgcgccg agatcgccac gggcgtgccg gaggcgctcg aatccgtcaa
     7561 gctcgctctc gggcggatga aaacgatcat cgattcgtgt cccgacgaag tggagcacat
     7621 cggcaacttc cccacgctgc acctcggtac tgttacgccg accggcgagc tggaatacta
     7681 cgacggcgtc atccggatcg tcgatggcga gggtaacatc gttgccgacg gcctcgaccc
     7741 gcagcggttc catacttacc tcggcgaggc cagcgaagag tcgagctaca tgaagttccc
     7801 gttctacaag ccgctcggat atccgggagg aatgtaccgg gtcggcccgc tggcgcgcct
     7861 caacatcgcc cgcgcggccg gcaccgctct ggctgaccgt gagttgcgcg agttcaaaca
     7921 ccgttccacg ggcgcggtca accagtcctt ccactaccac ctggcgcggc tgattgagat
     7981 gctgcactgc gtagagcgca tagaggagat tgcttccgat cccgttctcc tcaagacgcg
     8041 cgttcgcgcc gtcgccggcc agaaccgcaa tgaaggcgtc ggttcgagcg aggctccgcg
     8101 cggcacgcta ttccaccatt acgaagtcga cgataatggc ctgatccgca aagtgaatct
     8161 gttgatcgcc accggccaaa ataaccttgc gatgaacaag accgtggagc agaccgcgat
     8221 tcggttcgtg aatccgcgcg cgctccgcga ggagatgctg aaccgcgtgg aggccgggat
     8281 tcgctgcttc gatccctgcc tgtcgtgttc tacgcacgcg gccggacaga tgcctctcat
     8341 gctcgagatt cgcaaccggc gcggcgaggt cctcggcagg cttgcccgaa gatgaccttt
     8401 ctgatcgcgg tgggcaacac actccgtcgt gacgacggcg tggcccaccg cgtcctcgaa
     8461 ttgctgaagc cacccgccgg cgtcgttgtc cgctcctgcc atcaactgac ccccgaaatg
     8521 gccgaagaaa tcgcgtccgc ggacacggtc atcatcatcg acgctgacgt ggctcccgga
     8581 gagccgcgcc ttgagaaggt cgaagagcgc ccagacaacc cgctcacgca cgtcgtgcgg
     8641 cccgccgagc ttgtggcgct ttcgcgccgg cttttttcgt ttcgcggcga agcctggctc
     8701 tgccgcgttc ccggcatcga tttcggacag ggctccggcc tcagccctgc gacggaggcc
     8761 aacgcctgca tcaccgccga gctcctcctc cgcttcctcg gctgacacgc tgcatcttct
     8821 gcgcatcgaa ccagtccgtc gcgcaacccg tgcgtcgttc acccatgtcg gtcgctcgta
     8881 accatctgat tcttagactg ttccactatg gcgtgacccg tgcgctccgg tcgtgcggac
     8941 gtcaggtccg aagagtaaaa aggagaacgt tatgaaaccg aactatcgct tgctggttgt
     9001 cctgtttgtg gtcgctgcgc ttgccgtcgc gtgggcgcag ggtccccgcg ggcgggccca
     9061 gagtgcaccc ggaaacggcc agggacaacc cgcgctggac atgacgaaac tggtcacggt
     9121 ggagggaccg gtttcggcga tacaactcaa ttatggagcg cagtacccca caatcgaagt
     9181 caacaagacc ttgatcaaga tcgcaccggt gtggttcctg atcgagaatg acttcgaaat
     9241 cgacgtgaat gatgtcgtca aggtaacggc ggcgctgtca accgccgacg cttacctgca
     9301 cgccgtcgag atcgtgaatg tcaccaacag caacgcgctg attacgctgc gcgacaaaag
     9361 cggaacgcct ctgtggacca cgcggcaggc cggacgcgct gggcgctgga gccaggaccg
     9421 gatgttgcgt ggaaacggcg gcggacaagc gtgcctcgac atggcctctg gcaccactgt
     9481 ttcgggcatt gtaagccagg tgacgatggg aaccggcatc aagcagcccg ttgtcgtgat
     9541 cgaacaggcc gacaagtctc tcatcagcgt gaagattggg ccggagcgga tcctgctcga
     9601 aaacgatgtc gaactgaccg ccggcgaagc ggtgaccgcg aagatcgcca agtctgcctg
     9661 caaggacgaa tgggtagcac tcgagctcac ggtggatggc aagactgttg cgctgagaac
     9721 ggaactcggc gcaccggcct ggaattgacc ccaaccaagt ctcactcgcc cttcggcgat
     9781 acggcccgca agccgtatcg ccttttatct tgatcatcga catgggagaa tcttcgtttg
     9841 atctatcttt ggattgctct cggcagcgcc atcggcggtg ccgcccgcta ctggtcctac
     9901 ggcatctcgg cccgcctctt cggcgagacc tttccatggg gcacactggc cgtcaacgtc
     9961 atcggatccg ccttcatcgg tttcttcgcg acgttgacct ctcccgacgg ccggctcttg
    10021 gttccgtcgt ccacgaggca gttcgtcatg gccggtgtct gcggcggctt cacgacgttt
    10081 tccacgttca gcctggagac cctcaacctt gcccgcgacg gggagtgggg gcgggtgctc
    10141 ggcaacgtcc tcgggacagt gatcttgtgc ctcgccggcg tctggtgcgg gtacgcgctg
    10201 gcgacggcgc tcaacgaaag gtagaggtgc gcgatgcaga ttccacggga cgcggttttg
    10261 ctgcggatct tcctcggcga aaatgaccgc cacgggcacc agccgctcta cgaagcgatt
    10321 gtgctaaagg cgcgggagat gcacctcggt ggggctacgg tgcttcgggg accgatgggt
    10381 ttcggccact cgagccgcct gcacacggcg aaaatcctgc gcctgtccga ggacctgcca
    10441 attgtggtcg aaattgtcga ttccgaggcc aaaattgacg cttttctgcc cattttggac
    10501 accatgatcg gcagcggcct cgtcaccctc gaaaaggtca ccgtcctcca atacggcaaa
    10561 gccggaaagt gaacctgttt ggcaatttac tgctgtcggc catgccgagt gttcacgcga
    10621 gatccgcgcg cgaatgctga agggcgggct gagctagtgg tggaccgaga tggcaacgcc
    10681 cccgacctgc accccacctc agccgacaaa tggagagaaa ccggacgtcg cgattggtga
    10741 gtaactgttc gagggttttt ccacgcactc ttgcagcgaa cacaagccgt tagggtttct
    10801 cctggtgaca tccgcaatga caactggggg attcacccga gttgagcttc atgagatcat
    10861 gaagctggtg caggtgcatc gcgagatgct aatcgagggt tggtatgagt tcttcggccg
    10921 ttgaagtaca agaggcacgt gcgcagtcgg taaaggtcac cgacgacgcc ttgtccgtcg
    10981 atttagtgga tggccgtact atcatcgtgc cgctggtgtg gttcccccgc ctttggcatg
    11041 gtacggcgca ggaacggtat cgcttcgaga tcttcgggga cggcgcttac atccactggc
    11101 ccgccctcga cgaagatctg accgtctcgg gattgttggc cggtcgtcag tccggtgaga
    11161 gccccgagtc tttgcggcgg tggttaactt ctcgaaagat cagacgcaaa ccgcgagcct
    11221 cgaaagagtt gtcccgccaa tcggcagcgc cgccgcgcaa ggcagccagg cgcggctgaa
    11281 tccagaagtc ccagagcccc ggatgtttcc gggagaagcc gatccggcaa tgaaacgtgc
    11341 tttcgcggac gtggtctgcc gcatgctacc cccggagtgg acgcgcccgc gccggccctt
    11401 gcggctgccg ttgtccagca gatcgcggcc gcacggatgg taaacagagg attcttcctc
    11461 tcccagggac gaacccctta ttgcagtctg gaagaaatgg ttcaggccgt cgaactcgac
    11521 aacctgcaca tcggggttct ttccgtcagc cagcgcccct tttgatggct gcgaggttct
    11581 ctgttggggc agatcgaaga gatctttgcg ccgtgcgtcc tggacctttc accgactggc
    11641 tcagggatca cacaacgacg cgggctggcg gctgaagtgc gcagcaggat cattttttga
    11701 tcaacaattt cttcaaggca tcctgatgtt cggcagccca cttttccgcg gcgtgtgtgc
    11761 gcggaaacaa cgaggggatc gcaacggaat gcgccccttt gcgtagttcc cgcgagagtt
    11821 caattgtcac cacgttttca tagttatgga aatggtagag atacatgatg cggcgtttca
    11881 ggtcgtagat attcgaatac tgagtgggat tgtttccctc ctggtgcgtg gccgcgagaa
    11941 tgcgccggaa caagtcgatg tttgtgttgt ccccagcact tttcagcatc cttgtcgcga
    12001 tccgaaaccg ttcgcacgga gcctcacccg gtctggtcgc ggactgccga aagttagtct
    12061 ggacaaggaa tctccccttc ttgcgcagga tggtgttgcc gtcgaagatg gccgcatccc
    12121 cgctcgcatc gacgaacatc agaacggact tttccgccca gggaaactcg tacttccgga
    12181 agagtccgat aacgtcctcg atgcttgcac actctgccaa gaaccgatcc agcgctcccc
    12241 ctggaaatgg cctgttcggc acgcggttcg ccacgggttg cagcggcggt ttggtgcttg
    12301 gcggaagctt gacgagcggg acgccgaaac cgtcgaagaa aagaccgcga tcgttcatcc
    12361 cgccttgtgg ccaggcgtag tcatatccca cgtacatccg gccgtgttcc ccgttctccg
    12421 ccggaaagaa ccaaagccgc gtgtgcgggt cgaacagatc ctcgttgttg ccgacgagca
    12481 cccgcccttt tgcggcagca cagaatgcgg tgcaggcgtg cgccggcggc ggaagcacta
    12541 gcaggagaag ggcaggtatt aagaaagtca ggcgcatcgt gtcctgattg atgttacgca
    12601 gtgagggcag ccggtgttcg cagatccttc actcaagttg ccagcatcgt tgtgattggc
    12661 ggagctccca attcgttcca cttgtggcgg ttttcaggag agcggcgacc aatttctcaa
    12721 acccggcgaa attggagtcc gtcgccgtaa ttacctagaa tgagagaacc tggatcgtgt
    12781 cggaatcacc gcaacaggat cggctcaatc aggacctcgg gctcggcggc aagatgtccg
    12841 agcgcagccg cgcccgcctc ctcaatcgcg acggcacctt caacgtccgc cgcaacaacc
    12901 tgagctcgtt ccatccctac aacgcctata acacgcttct ctctcttccg gttccccgcc
    12961 tgctcgccct cgtggctgcc ggctacgtcg ccaccaacct cctgttcgca tccctgtact
    13021 ggcttgccgg ccctgacgcg ctctcgggcg ccgccccctc accgcttacg cgcttcgagg
    13081 actgcttctt tttcagtgtc cagaccctcg ccaccattgg ttacggcaag ctcaccccgc
    13141 acacgcgggc ggccaacttc ctggtcgcca tcgaggctct tgtcggtctc ctcggcttcg
    13201 ccatcctcag cgcactgctg ttcgcacgct tcgcccgtcc caccgccaag atcacgttca
    13261 gccgcaacgc cttgatcgcg ccctacgaga acggttgggc gctgatcttc cgcctcgcca
    13321 gcctccgcaa ccacgagctc accaatgtcc acgccatcgt gagcttcgcc cgctggattg
    13381 aagtgaacgg cctgcgtcgc cgccgtttcg atcaacttcc cctcgagcgc gacttcatca
    13441 tctttatgcc cctgcactgg gccgtcgtcc accccatcac cgaaaccagc ccgatgcgcg
    13501 gcttcacccc cgaatctctg gccgccgccg accccgaaat cgtctgcctc atcaacgctg
    13561 acgatgaaac cttcgctcaa accgtccacg gcaaaaccag ctacgacaag agcgacatcg
    13621 tctggggcgc ccgcttccgc gacatgtacc tccccgatgt tgacaaggtt gccatcgacc
    13681 tcaaccggct tcacgacttc gagcgggtgg cgccgcccag ttctctctga ccggattgtc
    13741 aaagcagaat gaggcccttg cgaatcccga tggtgacggc ttcggtgcgg ctggaagcgc
    13801 cgagcttggc aagaatcgag gccacgtgga acttcaccgt gtgctcggag attcccagct
    13861 tccaggcgat gatcttgttt gccgcaccct cggccatcat ccgcagcacc tccagttccc
    13921 gcgccgtgag cgcggagacg tcaccggcag cc
//
LOCUS       DATIFQ010000137        22305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1963487, whole genome shotgun sequence.
ACCESSION   DATIFQ010000137 DATIFQ010000000
VERSION     DATIFQ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 22305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22305
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963487"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VLE22_06240"
     CDS             complement(<1..852)
                     /locus_tag="VLE22_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSB14038.1"
                     /translation="MKNAGAEAATGAARRFETLSGIPVEPVYGPEHLSGFDTARDLGA
                     PGEFPYTRGIHRDMYRGKLWTMRQFSGFATPEETNRRYRYLLEQGQTGLSVAFDLPTL
                     MGFDADHPTSLGEVGKCGVAISSLEDMETLFEGIPLGDVSVSMTINSPAPVIWAMYLV
                     VAEKQGVLWERLDGTLQNDILKEYIAQKEYIYPPRPSMRLVTDVIEFAARRVPRFNPV
                     SISGYHIREAGSTAVQELAFTLRDGVEYVDWALERGLEIDEFAPRLSFFFNAHNDFFE
                     EIAKYRAA"
     gene            complement(912..4589)
                     /locus_tag="VLE22_06245"
     CDS             complement(912..4589)
                     /locus_tag="VLE22_06245"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HSB14039.1"
                     /translation="MSSKSEEKLGVNSWLEDELYQQYLHDRAAIDESWKRVFDADGGT
                     AAATPRSDSATSRPTPSAPPLQPGPGEEVVPLRGAAARIVDNMTASLGIPTATSQRII
                     PVKVIDENRHIINHHRTLVGKSKVSYTHLITWAILKALDSYPVLNHAYAEREGESFRV
                     IRKQINFGVAIDLPGKDGSRSLVVPNLKNAGALDFQEFLNAYDGLIARARSGKLAIED
                     FQGTTVSLTNPGTVGTVGSVPRLMAGQGAIIATGAIGYPAEYQGAAPEVRAMLGIGKV
                     MMITCTYDHRIIQGAESGMFLARVHALLQGEDGFYDRIFADLKMPHGPVRWESDRAAS
                     VPGAGGGRAAEIAKEAAVLQLINAYRVRGHLIADLDPLGSEPSYHAELDPATYGLTIW
                     DLDREFLTGSLAEAIGEKSPQPVATLREILETLRRTYCGKTGCEYMHIQHPEQKRWLQ
                     MRMEPETDNRPLDRDTRERILARLTEAEEFEHFLHARFVGHKRFSLEGAETAIPILDE
                     LLNRAAANGVVEVVMGMAHRGRLNVLANIVGKPLTQVFSEFEGEIDPASAQGSGDVKY
                     HLGARGAHVSPAGQQIVVSVAPNPSHLEAVDPVVEGIVRPKQERLGDTERERVIPVLI
                     HGDAAFAGQGVVAETLNLSQLHGYRTGGTVHLIINNQIGFTTMPEEARSSPYSTDVAR
                     TVQAPVFHVNGDDPEAAIRVTQIAFDYRQRFKKDVVIDMLCYRRHGHNEADDPSYTQP
                     ILYRKIKEHPSAAALYAERLIREQVLSAEAVEEMRKRATTRLSAAYDASQTHAEQYEL
                     QELTVVGEEEAGAWRPQTAVDQSVIERVVEGITTFPADFHLHPKLRGFIDRRRDGLRK
                     GGPIDWAFAESLAFGSLVLDGTPVRLSGQDSGRGTFSQRHLVLYDYETAREYTPLQHV
                     SDGQARFEVFDSSLSEYAVLGFEFGYSVGDPLTLVLWEAQFGDFGNGAQIMIDQFISC
                     AEQKWGQPTGLVMLLPHGFEGQGPEHSSARIERFLVLCAESNMQVCNCTTPAQYFHLL
                     RRQMYGGQDRRGVRKPLILFTPKSLLRHPKVVSSIPDFTGGGFREILSETGAIDPQSV
                     SRILLCSGKVYYDLIEAREQREAGQVAIVRVEQLYPFASSQLGDILLKYPPAADVFWV
                     QEEPRNMGPWRFMRDRIQPLLDPTERMLRFAGRPESASPATGSHKRHAAEQAALIEEA
                     FAPAAMPSRSAARIVARRKAK"
     gene            complement(4608..5585)
                     /locus_tag="VLE22_06250"
     CDS             complement(4608..5585)
                     /locus_tag="VLE22_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14040.1"
                     /translation="MYYSLSMFNWLVIGIGDITRKRVIPAIQLQGRSRLYGIVTRDRR
                     KAEPYGVQAWTSLDEALVDPLIDAVYVAAPVFLHAPQTIASLRAGKHVLCEKPAAMNF
                     TEAREMVRTANEAGRTLGIAYYRRMYPKLQRARQLLAEGAIGRPVLAEINCHDWFNDE
                     DGTRSWLLDPRQAGGGPLYDIGSHRIDVLNFLFGRPVRVTAQLSNVVHSAPVEDNATV
                     LIEYESGVRGVVDVRWHSRVSRDEFRIAGTDGEMELSPLNGPLLIYAGGREELRAHAN
                     LHYPCVDNFVNAVLDGTPLVADAESAVWTDWVTEQALVAARVGSLGYHE"
     gene            5595..6203
                     /locus_tag="VLE22_06255"
     CDS             5595..6203
                     /locus_tag="VLE22_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSB14041.1"
                     /translation="MKLLVFSDIHSNHGALERLMDVEADCYIAAGDLVSWSRGLDRCG
                     EILSRRTDRMYVLPGNHESAASIASFCRKFGFHDFHEQSMELAGFQIAGLGYSNPTPF
                     NTPGEYSEQEIELRLERFANLKPLILICHCPPFGTALDRIRNGLHAGSRSVRTFIEKH
                     QPVFFFCGHIHEAEGASAEIGKTRGINVGQQGYLLDFATLKV"
     gene            6249..7349
                     /gene="prfB"
                     /locus_tag="VLE22_06260"
     CDS             6249..7349
                     /gene="prfB"
                     /locus_tag="VLE22_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HSB14042.1"
                     /translation="MKLCGAIFDSPKKKAELTRLEERISSPDFWNQPEKSQQVMQERK
                     RLEQSIADEARIAGRISDFDAVFELAREGNTEGALVLLGDPLDELKKFRHDLDRIETA
                     MLLSGENDARSAIVTIHPGAGGTESQDWAEMLLRMYLRWAEREGFGTVVTDRLEGDGA
                     GIKSVTFEINGEYVYGLMQSEVGVHRLVRISPFDANARRHTSFASVFVYPQIDDEIKI
                     DIRPEDLRVDTFRASGAGGQHVNRTDSAIRMTHLPTGIVVQCQNERSQHKNRASAMKQ
                     LRARLYEFELEKKQAEDSKLEATKLDINFGSQIRNYVLAPYRLIKDLRTKLAIGDVDR
                     VLDGDLDDLIHAHLVFRKTGRTAGDGKDELPE"
     gene            7333..8322
                     /locus_tag="VLE22_06265"
     CDS             7333..8322
                     /locus_tag="VLE22_06265"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HSB14043.1"
                     /translation="MSCPSSPVETVTPETLARAAELIRAGGIVAFPTETVYGLGANAL
                     DEEAVRRVFEAKGRPSTSPLIVHVESVEMARGLVRDWPPSAEALARQYWPGPLTLVLE
                     KQPHVPGIVTAGLPTVGLRMPAHPLALSLIRAAGVPIAAPSANRFAKLSPTTARHVRD
                     ALGGRVDLILDGGPTEVGIESTVLSLAASPPRLLRPGRVTQTDIEAVIGSIEIAGRIE
                     NGDNDGHPSPGLHPKHYSPATPLVLVDGGPLPAGRGVYLYRERHAQAARCVAMPTDAG
                     AYAAVLYETLHREDAAGWDWIAVERPPEGPEWAGVTDRLTRAESKRSGSGDKS"
     gene            8319..9167
                     /locus_tag="VLE22_06270"
     CDS             8319..9167
                     /locus_tag="VLE22_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB14044.1"
                     /translation="MIQPVRKGRELLDEIDNEAAQTPVLWWLGQSGFVVKYRRIVFYL
                     DPYLSESLTKKYGNTNRPHIRMTEAPFRGEDVIHADLVFSTHGHSDHLDPGTVPAILA
                     SSPEARLVLPKALVEKACAMGVPSERMRPTDAGDVIEFRKDGAVATIHPIPSAHEQLD
                     WTPESGYPYLGYVIRFGEWTIYQPGDCVPYDVLVERLRPFGVTVALLPVNGRDPARGV
                     AGNFSISEAAQLAEDIGARWLIPAHYDMFTFNTVAIGLFVEHMKIRPSQNFKILRCGE
                     RWVVPR"
     gene            complement(9193..9600)
                     /locus_tag="VLE22_06275"
     CDS             complement(9193..9600)
                     /locus_tag="VLE22_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB14045.1"
                     /translation="MPFVLDASVAASWAFQDEDHPAADLAFEHFRSDEAVAPSLLWFE
                     LRNILVVSERRKRLGQADTTAFLREFSRLPLRVDRSPDELEVLRLARSYQLSVYDAAY
                     LELALREGLPLATLDTTLAKAAQAEGVRLIGVT"
     gene            complement(9600..9845)
                     /locus_tag="VLE22_06280"
     CDS             complement(9600..9845)
                     /locus_tag="VLE22_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HSB14046.1"
                     /translation="MREIQASDAKTHLPRLLDAVERGETVVITRHGRPIARIVPEANR
                     RQREIDDALARIANLRKRTGRITSDELLSARDEGREG"
     gene            10129..10458
                     /locus_tag="VLE22_06285"
     CDS             10129..10458
                     /locus_tag="VLE22_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14047.1"
                     /translation="MVRVKRAGQVAAFEFGVSAEGYSALRRMFNARPFDAMPGLKNRY
                     FFTGTYGRRKLGEKPAFFQVRIEQGWTAKLFEFPGPTSLVANLLWFKEMKTLEAASAL
                     KRVDTAL"
     gene            10597..12150
                     /gene="purH"
                     /locus_tag="VLE22_06290"
     CDS             10597..12150
                     /gene="purH"
                     /locus_tag="VLE22_06290"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HSB14048.1"
                     /translation="MAKIQRALISLTDKSGILEFARGLAELGVEILSTGGTARMLRDA
                     GLAVRDVADVTGFPEMLDGRVKTIHPRIAGGILAIRSNEEHMQALAAHGIPTIDMVVV
                     NLYAFEKVAAREGATVPELIENIDIGGPTMIRAAAKNYQDVAVVVSPADYDPILTELR
                     ASGGLSLATHWRLAKTAYRLTADYDRAVSARLARIEVREGAFADSTPNLPPVLDLRAD
                     LALPLRYGENPHQSAALYSDRRSGIAGAEQLQGKELSYNNLVDLDAAWQLVSEFGDPA
                     SAIIKHTNPCGCAEAASLAESYRKALECDPVSAFGGVLAFNRPLDEETAREIAKTFIE
                     AIAAPDYSQEALAVLAAKKNLRLLHVTNPAPDPVVIKSISGGFLAQTADLHRLDRAQI
                     QVKTKRVPTEEEWAALEFGWKVVKHVKSNAIVFARAGQIAGVGAGQMSRVDSVKVAAM
                     KAVLPLQGTALASDAFFPFPDGIEEAARYGATAVIQPGGSVKDAEVIAAANRLDLAMV
                     FTGIRHFRH"
     gene            12170..13081
                     /locus_tag="VLE22_06295"
     CDS             12170..13081
                     /locus_tag="VLE22_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB14049.1"
                     /translation="MRREFLSRRRLLKASILAAAGAAAQTQGKATEFQIACMTLPYAA
                     FPLERALKGIAGAGYRYVAWGATHKESDGQTRQALAVDDPPSAAKELGSRSRDLGLEP
                     VMMFSLVNVEAPDSIKSHTRRIEQAAAAGVPFVLTFGHTRIASYETWIRNLKELGPIA
                     RNAGVTIVIKQHGGNSATGKLCAQILREVFDEGIQMCYDAGNNLGYVGDDPIADIRHC
                     WQYVRAFAIKDVRLTPKRDACGPGLGEIDHYKLLLPVARTGLKIPLCCETLWAPLLPR
                     PEQPEGVDALAKRAREFLEVVTVGVTA"
     gene            complement(13100..13555)
                     /locus_tag="VLE22_06300"
     CDS             complement(13100..13555)
                     /locus_tag="VLE22_06300"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HSB14050.1"
                     /translation="MPTTTALLLALLIAPTGFAGTADASVLLDADRAFAVATEKGRLE
                     GFLSFLAEDTATLRPDLPVIRGRKAVAERWASLLSNPAMSIRWQPLDAAISSSGDLGY
                     TIGAYEIVRSGENAGVVATGKYVTIWKKQADGSWKVVFDSGVPDTPKNK"
     gene            complement(13560..14297)
                     /locus_tag="VLE22_06305"
     CDS             complement(13560..14297)
                     /locus_tag="VLE22_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB14051.1"
                     /translation="MSELVIETRDLEKEFHRDGFGITALRMINLTVHKGEFVALMGPS
                     GSGKSTLLHLIAAMDRPTGGEIRVLGENLRDLSDGEIADWRNTHIGFVFQTFNLIPVL
                     TAFENVELPLKLTHLKKRERIAHATTALKLVGLGDRLHHLPRQLSGGQEQRVAIARAI
                     VTDPDLILADEPTGNLDAGSAQEVLSLLSKLNKEYGKTIVMVTHDPHAAHFAVKIRYL
                     EKAELLPEGQVPADWAMPARSVKRTGE"
     gene            complement(14302..15630)
                     /locus_tag="VLE22_06310"
     CDS             complement(14302..15630)
                     /locus_tag="VLE22_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB14052.1"
                     /translation="MDADLKSLQIERKHRDKGEPKWATRWIVAGVAFFLLLGLGRFLY
                     SRMNAATEVSVVRVSASGGAGDNGRNTILNATGYIVAAHKIEVASKVIGRVAWIGVDK
                     GDKVKERQVIVRLEDDEYRAQLQQARGQLTNLEARLQELLNGSRPEEVAVAKANLEQS
                     RADLENARTNLDRIRKLHAEKVTSQQSLDDAQARYDAQAARVASLEKSYELVRIGPRQ
                     EQIDAMRGQVEQARGMVAFYETQLANTVIRAPVTGTILERVVEKGEFVTTSFVGERGA
                     KGYVVSLADLNDLEVELDISQNDFAKLGAQQRGVVTTDAFPDRKYEGVIDEISPEANR
                     QKATVQVKVKILHPDEYLRPEMNASVAFYEEGATGAPVRPSIVIPASAVRDGAVFVTL
                     GEKAVRRAVRTGATTNDGIRIEQGLTGGEDVIVNPPSELKDGDRVRIRKG"
     gene            complement(15640..16806)
                     /locus_tag="VLE22_06315"
     CDS             complement(15640..16806)
                     /locus_tag="VLE22_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14053.1"
                     /translation="MKIPLTYNLRNLVVRKTTTVMTALGVALTVAVLLSVLALLDGLR
                     IAFQSTGNPLHVLILRKGSESELVSNFTREMYQVVRAKPGIQRGKSGEPRASLEMVTV
                     VNLPRVDSPEGMNVTIRGLMPVGIEMREDLKLLRGRWFESGRREVVVGSSIAKRFPGA
                     EVGKKIHAGSGDWDVVGVMEAGTGVSNSEIFADLNQLSSDYNRTEVVSSALVEASDEV
                     AAQALINDLNSDRRLNVEARTEKDYYAAQTVSGAPIQYIGTLVAIIMAVGSSVAAMNT
                     MYAAVARRAREIGTLRVLGFSRGSILFSFLIESVLLALLGGLIGVLLTLPLNGVTTGI
                     GNFVTFSEIAFNFHVGPKTITTGLIFAMLMGAVGGLFPALMAAKKEILIALREI"
     gene            complement(16823..17980)
                     /locus_tag="VLE22_06320"
     CDS             complement(16823..17980)
                     /locus_tag="VLE22_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14054.1"
                     /translation="MRYLPFIFKNAWRNRRRTALTVLSLGVSLCLLGLLMAIYQAFYL
                     TEASPEQARRLIVRNRVSLAVPMPIYYRERIRQIPGVQEVMISQWFGGVFRDSRDPEN
                     FFARFAVEPEKLFTVRGEIRVPEDQKRAFLAERTACAIGRPLAERHNLKLGDRITLKG
                     DIFPVDLELTVRAIWEAAQDDEVLYFHWKYLEEGLPNRRQGMVGTFTILAASPDAVGG
                     IAAAVDETFRNAPFQTRTETEQAFALSFLAFLGNVKAFLLSIFAAVTFTILLVSANTM
                     AMSVRERIREVGVMKTLGFKPGGIVGIILGEATVISLAGGVLGCLLAAGLVSAIRNGP
                     AALAMLRNLAVGPPVVAVSLSIAVFVGLVSAAVPAWSASRTSIVDALRHAG"
     gene            18039..18485
                     /gene="tadA"
                     /locus_tag="VLE22_06325"
     CDS             18039..18485
                     /gene="tadA"
                     /locus_tag="VLE22_06325"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HSB14055.1"
                     /translation="MTDETWMLEALALAREAGSEGEVPVGAIVVCGGVIVGRGRNSPL
                     ARVDPTAHAEILALREAAARLENYRLEDCTLYVTIEPCVMCAGALVTARVRRLVFGAR
                     DLRFGGVRSKFRVADSDLLNHRVDVEEGVLATDCAELLREFFRERR"
     gene            complement(18601..19158)
                     /locus_tag="VLE22_06330"
     CDS             complement(18601..19158)
                     /locus_tag="VLE22_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14056.1"
                     /translation="MARHSCSHCHGLGLRQGHRERMYPCNCVLRAIFRACYARFRYCA
                     AKEKYLSRVSLEKISGREGRWSWGRKDEEYVADFCLVSRRMLTEYEHMVFRYHFLLGA
                     DWKLCCRKLKMDRGNFFHSVYRIEQTLGRAFRELEPYPLYPLHDYFHGTMLTEPDRKG
                     PASSALAAPLRAARPVFHPPLLKTA"
     gene            19441..20139
                     /locus_tag="VLE22_06335"
     CDS             19441..20139
                     /locus_tag="VLE22_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14057.1"
                     /translation="MGPTMDFQELQARLVAALKARLHNGELTERRLARLAGISQPHMH
                     NVLKGARILSPDAADRVLKCLRLSVLDLITVPELHQAGCPQCPKHGAYRGVPILEGWL
                     GPGLPLPTATGPEYRCPFPTLLVTSLTDPVVARLTADARMTAVVRHNDLVLLDRSHHS
                     RSRLDGEGLYVVNRHGEGLIRRLRMEGRRVFLGTEAGLGRPCEWEEIVLENSHVMDIV
                     RARVVWIGRYLLSF"
     gene            complement(20173..21351)
                     /locus_tag="VLE22_06340"
     CDS             complement(20173..21351)
                     /locus_tag="VLE22_06340"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSB14058.1"
                     /translation="MALRILIDARHLRDFGIGTYIRNLVRALACIDRENHYVLACGKE
                     GGAELAGLPGNFKIARYERHDSDPLDDVAFPWFLRHFSADLYHIPLNAVSLAMPQPYV
                     VTIHDMSSLMYDRQRDFWRRLRLYRFRRGLQRAEKVIAVSEATLRDVEDLFGILPERV
                     RRIYNAPDPRFLEHKPAADARSAGPEGHSREVRRILERYQIDYPFVLYTGSIRPQKNV
                     PRLVEAFAVVRGQLETHPVYKDLRLIIIGDELSRYPAVRRAVIQTRVEDVVRFLGFVP
                     IDTLRVFYEAAAAFVFPSMYEGFGLPPLEAMASGTPVVSSNRSSLPEVVGDAAALVNP
                     ENVFDIARGIRDVLTDEQLRVSLIARGYEQARRFSWESTARQVLEVYEEVGRERKVLR
                     "
     gene            complement(21352..>22305)
                     /gene="glyA"
                     /locus_tag="VLE22_06345"
     CDS             complement(21352..>22305)
                     /gene="glyA"
                     /locus_tag="VLE22_06345"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSB14059.1"
                     /translation="AVLQPGDTILGMNLAHGGHLTHGHHLNFSGKTYKVVPYGVRRED
                     EIIDYDELARLAEEHKPKMIIAGGSAYPRIIDFRRFREIADAAGAIFLVDMAHFSGLV
                     AAGLHPNPCEYADIVTSTTHKTLRGPRAGIILSRAQFAQAIDKTVFPGVQGGPLVHVV
                     AAKAVCFAEAMTPEFVSYQRQVKANAAALADGMAEAGYRVVSGGTDTHLLLVDVFARA
                     IRGRDAETGLDKAHITVNKNAIPFDANPPLNPSGIRLGSPAVTTRGFREPEMREVASM
                     IAEVLGNLGNDDVIASVRRRVAELTGRFPLYHWKLNAVAAN"
BASE COUNT         4080 a         7211 c         6799 g         4215 t
ORIGIN      
        1 cgccgcccgg tacttcgcga tctcctcgaa aaaatcgttg tgggcgttga agaagaagct
       61 gagccgtggc gcgaactcgt cgatttccag tcccctttcg agcgcccagt cgacgtactc
      121 cacgccgtcc cgcagcgtga acgccagctc ctgcaccgcc gtcgatccgg cctcgcgaat
      181 gtgatagccg ctgatcgaga ccggattgaa cctcggcacg cgccgcgccg caaactcgat
      241 gacgtccgtt acgagtcgca tcgaagggcg cggcgggtag atgtattcct tctgtgcgat
      301 gtactccttc agaatgtcgt tctgaagcgt gccgtccagc ctctcccaga ggacaccctg
      361 cttttccgct acaacaagat acatcgccca gatgacaggc gccggcgagt tgatggtcat
      421 cgagaccgaa acgtcgccga gcggaatacc ctcgaacagc gtctccatgt cctcgagcga
      481 actgatggcg acgccacact tgccgacttc gccgagcgat gtcggatggt cggcgtcgaa
      541 gcccatcagt gtgggcagat cgaatgcgac ggaaagcccg gtctggccct gctcaagaag
      601 ataccggtag cgccggttcg tttcctcggg ggtggcgaat cccgagaact ggcgcatggt
      661 ccagagtttt ccccggtaca tgtcgcgatg gattccgcgc gtgtaaggga actctccggg
      721 agcgcctagg tcgcgagcgg tgtcgaaacc ggaaaggtgt tccggcccgt acaccggctc
      781 cacgggaatg ccgctcagcg tctcaaagcg ccgcgcagcc cctgtggccg cctccgcccc
      841 agcgttcttc atctgtcttc tattgtacct gtcggattgg cgagtacttg gggcgcccgt
      901 ttggtcttca ctcactttgc cttccgccgg gcgacaatgc gggcggcgct cctgctcggc
      961 atcgccgccg gggcgaaggc ctcttcgatc aatgcggcct gctcggcggc gtgtcgtttg
     1021 tgggaaccag tagccggact ggcgctctct gggcgccctg cgaagcggag catccgctcg
     1081 gtcgggtcga gcaacggctg gatgcgatcc cgcatgaagc gccacgggcc catgttgcgc
     1141 ggctcttcct gcacccagaa cacgtccgct gccggcggat acttcaaaag gatgtctccg
     1201 agttgcgacg aagcgaacgg atacagttgc tcgacgcgga cgatggccac ctgcccggct
     1261 tctcgctgct cccgagcctc gatcaagtcg tagtagacct tgccagagca gagcagaatt
     1321 ctcgacacgc tttggggatc gattgcgcct gtttcgctca gaatctcgcg gaagccgccg
     1381 ccggtgaagt ccggaatgct cgagaccacc ttcgggtggc gcaacaggct ctttggcgtg
     1441 aacaggatca gcggcttacg gaccccccgg cgatcctgcc cgccatacat ctggcggcgc
     1501 agcaagtgaa aatactgggc cggcgtcgtg cagttgcaca cctgcatgtt gctttcggcg
     1561 cagagtacaa gaaaccgctc gatgcgggcg ctcgagtgct ccgggccctg tccctcgaag
     1621 ccgtgcggca gcagcatcac gaggcccgtt ggctgtcccc acttctgctc ggcgcaggag
     1681 atgaactgat cgatcatgat ctgcgcgccg ttgccgaagt ccccgaactg cgcctcccag
     1741 agcaccagtg ttaatggatc gccgacactg tagccgaact cgaatccgag tacggcgtac
     1801 tcgctcaggg agctgtcaaa aacctcgaag cgggcctggc cgtcggaaac gtgctggagc
     1861 ggagtgtact cgcgggccgt ctcgtagtcg tacaaaacga gatgcctctg gctgaacgtt
     1921 ccgcgcccgc tgtcctggcc gctgagccgg acgggcgtgc cgtcgagcac gaggctgccg
     1981 aacgcgagcg actccgcgaa cgcccagtcg atcggtccgc ccttacgcaa cccatctctg
     2041 cgccggtcga tgaatccgcg gagcttcgga tgcaagtgga aatcggccgg gaaggtcgta
     2101 atgccctcca ccacgcgctc gatcacgctc tggtcgaccg cggtctgcgg acgccacgct
     2161 ccggcttcct cctcgccgac aacggtcagt tcctgaagct cgtactgctc ggcatgagtc
     2221 tgcgatgcgt cgtacgccgc ggaaaggcgg gtcgtggcgc gctttcgcat ctcctcaacg
     2281 gcttccgcgc tgaggacctg ctcgcggatc aaccgctcgg cgtagagcgc ggccgcggac
     2341 gggtgctcct tgatcttccg gtacagtatc ggctgcgtgt agcttgggtc gtcggcctcg
     2401 ttgtgtccgt ggcggcggta acacaacatg tcgatcacga catccttctt gaaccgctgc
     2461 cggtagtcga acgcaatctg tgtcacccgg atggccgcct ccggatcgtc gccgtttacg
     2521 tggaataccg gcgcctggac ggtgcgtgcg acgtccgtgg aatagggcga agaccgtgcc
     2581 tcctcgggca ttgtggtgaa accgatctgg ttgttgatga tcaggtgcac ggtgccgccc
     2641 gtccggtaac catggagttg cgagaggttc agcgtctcgg ccaccacgcc ctggcctgcg
     2701 aaggcggcgt caccgtgaat gagcacgggg atcaccctct ctcgctccgt atcgcccagc
     2761 cgctcctgtt tcggccggac gatcccttct accacgggat cgaccgcctc caaatggctc
     2821 gggttcggag cgacagagac gacgatctgc tgtcctgcgg gcgagacgtg cgcgccgcgc
     2881 gcgccgagat ggtacttcac gtcgccggag ccttgcgcgc tggctgggtc tatctctcct
     2941 tcgaactcgg agaagacctg cgtgagcggc ttgccgacga tgttcgccag cacgttcaag
     3001 cgcccacggt gagccatccc catgacgact tcgacgacgc cgttcgcggc ggcgcggttc
     3061 agcaactcat cgagaatcgg gatggccgtc tcggcgcctt cgagcgaaaa gcgcttgtgc
     3121 ccgacgaagc gggcatggag gaaatgctcg aactcctcgg cctccgtcag tctcgccagg
     3181 atccgctcgc gtgtgtcgcg atcgagcggc ctgttgtcgg tttcgggctc catccgcatc
     3241 tggagccagc gcttctgctc cggatgttgg atgtgcatgt actcgcagcc ggttttgccg
     3301 caataggtcc ggcgcaaagt ctccaaaatc tcgcgcaaag tcgcgaccgg ttgcgggctt
     3361 ttctcgccga tcgcctcagc gaggcttccg gtcagaaact cgcgatccag atcccagatg
     3421 gtgaggccgt acgtcgcggg atccagttcg gcgtgatagc tgggctcaga gcccaacgga
     3481 tcgaggtcgg cgatcaggtg gccgcggacc cggtaggcgt tgatgagctg caggacggcc
     3541 gcttctttgg cgatctccgc ggctcggccg ccgcccgctc ccggcacgga agcggcgcgg
     3601 tccgactccc agcgcactgg gccgtgcggc atcttcagat cggcgaagat cctgtcatag
     3661 aatccgtctt cgccctgaag cagtgcgtga acgcgtgcga ggaacatgcc ggactcagcg
     3721 ccctgaatga tccgatggtc gtacgtgcag gtgatcatca tcaccttgcc gatgccgagc
     3781 atcgcacgga cttcgggggc cgcgccctgg tattccgccg ggtaaccgat ggcgcccgtg
     3841 gcgatgattg cgccctgccc ggccatcagc ctcggaacgg aaccgacagt accgacggtg
     3901 cccgggttgg tcagcgagac ggtcgtgcct tgaaaatcct cgatcgcgag cttgccggag
     3961 cgagcacggg cgatcaggcc gtcgtaggcg ttcagaaatt cctggaaatc gagcgcgcca
     4021 gcgtttttga ggttcggaac cacgagagaa cgggagccat ccttacccgg caggtcgatg
     4081 gcgacgccga agttgatctg cttccggata acgcgaaagc tttccccttc cctttcggcg
     4141 taagcgtggt tgagcacggg gtagctgtca agcgccttca gaatggccca ggtaatgaga
     4201 tgcgtgtaag agaccttgct cttgccgacc agtgtgcggt ggtggttgat gatgtgccgg
     4261 ttttcgtcga tcactttcac cgggatgatg cgctgcgatg tggccgtggg aatgcccagg
     4321 ctggccgtca tgttgtccac aatgcgcgct gccgcgccgc gaagggggac gacttcttca
     4381 ccagggccgg gctgcagcgg cggggcggat ggcgtgggcc gggacgtcgc ggagtcggag
     4441 cgcggcgttg ccgccgccgt cccgccgtcg gcgtcaaaaa cacgcttcca gctctcgtcg
     4501 attgccgcac ggtcgtgcag gtactgctgg tacaactcat cttccagcca actgttgaca
     4561 cccagtttct cttcgctctt ggaggacatt cgggcaggcc ttcttcttta ttcatgatac
     4621 ccgagcgaac ccacgcgggc cgccaccagc gcctgttccg tcacccaatc cgtccacacc
     4681 gccgattccg cgtcggcgac gagcggcgtt ccgtcgagca cggcgtttac gaagttatcg
     4741 acgcatgggt agtggaggtt tgcgtgcgcc cgcaattcct cacggccgcc cgcatagatg
     4801 agcaacggtc cgttgagagg actgagttcc atctcgccgt ccgttccggc gatgcggaac
     4861 tcgtcgcggc tgacgcggga atgccaccgc acgtcgacga cgccgcgtac cccgctctcg
     4921 tattcgataa gaaccgtggc gttgtcctcg accggagcgg agtgaacgac gttcgagagt
     4981 tgcgcggtca cgcggaccgg cctgccgaac aggaagttga gcacgtcgat acggtgtgaa
     5041 ccgatgtcgt aaagcggccc gccgcccgct tggcgcgggt cgagcagcca gcttcgcgtg
     5101 ccgtcctcgt cattgaacca gtcgtggcaa ttgatctcag ccagcaccgg ccggccgatc
     5161 gccccctccg ccagcaactg cctggctcgc tggagcttcg ggtacatccg gcggtagtaa
     5221 gcgattccga gggtcctgcc ggcctcgttc gcggtccgga ccatctcgcg ggcttcagta
     5281 aagttcatcg cggccggctt ctcgcagaga acatgcttgc cggcgcgaag cgaagcgatc
     5341 gtctgcggag catgaagaaa aaccggcgcg gcaacgtaga ctgcatcgat gagggggtcc
     5401 acaagggcct cgtcgagcga agtccaggcc tgcacgccat agggctccgc cttccgccgg
     5461 tcgcgggtga cgatcccgta gagccggctc cgcccctgca attggatggc aggaataacc
     5521 cgcttcctgg tgatgtctcc aattccgatc acaagccagt tgaacatgct gagagaatag
     5581 tacaatcacg cgaaatgaag ctcctcgttt tttcggacat ccacagcaac cacggggcgc
     5641 ttgagcggct gatggacgtc gaagccgact gctacatcgc ggccggcgac ttggtgagct
     5701 ggtcgagggg cctcgaccgt tgcggcgaga ttctgagccg caggacggac cgcatgtacg
     5761 tactgcccgg caaccacgag tccgcggcct cgattgcgtc cttctgccgg aaattcggct
     5821 tccatgattt tcacgaacag agtatggaac ttgccggctt ccagatcgcc gggctcggct
     5881 actcgaatcc gacgccgttc aataccccgg gcgaatattc ggaacaggag atcgagttgc
     5941 gtctggagcg gtttgcgaac ctgaagccgc tgattctgat ctgtcactgc ccgccgttcg
     6001 gcacggcgct cgacagaatc cgtaacggac tccatgccgg cagccgctcc gtcaggacat
     6061 tcattgagaa acaccagccc gtcttcttct tctgcggcca tatccacgag gccgaaggcg
     6121 cctccgccga aatcggaaag acccgtggaa tcaacgtcgg gcagcaaggt tatcttctcg
     6181 actttgctac actgaaggta tgaacatcga ggaactcgaa agagaatact cgccgctccg
     6241 ccagcaggct gaagctgtgc ggagctatct ttgactcccc caaaaagaaa gctgaactaa
     6301 ctcgactgga agagcggatc tcttcgccgg atttctggaa ccagccggaa aaaagccagc
     6361 aggtcatgca ggagcgcaag cgcctggagc agtcgatcgc cgacgaggcc aggatcgcgg
     6421 gccggatctc cgactttgat gccgtgtttg agcttgcccg agagggcaac accgagggcg
     6481 cgctcgtgct cctcggcgat ccgctcgacg agctgaagaa gttccggcac gatctcgaca
     6541 gaatcgaaac ggctatgttg ctctccggtg agaacgacgc ccggagcgcg attgtaacca
     6601 ttcatccggg cgcgggcggc acagaaagcc aggactgggc cgagatgctg ctccgcatgt
     6661 acctgcgatg ggccgagcgg gaggggttcg ggaccgtcgt aacggaccgg ctcgagggcg
     6721 acggagccgg catcaagtcg gttaccttcg agatcaacgg cgaatacgtc tacgggctca
     6781 tgcaatccga ggtcggcgtg catcgcctgg tgaggatctc gccgttcgat gcgaacgccc
     6841 ggcggcacac gtcgtttgcc tctgttttcg tctacccgca gattgacgac gagatcaaga
     6901 tcgacatccg gccagaggat cttcgcgtcg atacgttccg cgccagcggc gccggcggcc
     6961 agcatgtcaa ccgcacagac tcggcgatcc gcatgaccca cctgccgacc ggcatcgtgg
     7021 tgcagtgcca gaacgagcgc tcgcagcaca agaaccgcgc ctcggcgatg aagcagctca
     7081 gggcgcgcct ctatgagttc gagctcgaga agaagcaggc cgaagacagc aagctcgagg
     7141 ccacgaagct cgacatcaat ttcggcagcc agattcgaaa ctacgtgctc gccccctatc
     7201 gcctcatcaa ggatctccgc accaagctgg ccatcggcga tgtcgatcgc gttctggacg
     7261 gtgatctcga cgacctcatc cacgcgcacc ttgtcttccg aaagaccggc aggactgcgg
     7321 gcgacggtaa ggatgagctg cccgagtagt ccggtggaaa ccgtcacgcc tgagaccctg
     7381 gcccgcgccg cggagctgat ccgcgccggt gggattgtcg ccttccccac cgagaccgtc
     7441 tacggtctcg gcgccaacgc acttgacgag gaggcggtgc ggcgcgtctt cgaagcgaag
     7501 ggacgcccgt ccacaagccc gttgatcgtg catgtggagt cagtcgaaat ggcgcgggga
     7561 ctggtgcgcg actggccgcc ctccgcggaa gcgctggccc gccagtactg gccgggacca
     7621 ctgaccctgg tgctcgaaaa gcagccgcac gtgcccggca tcgtgacggc cggacttccg
     7681 actgtggggc ttcgcatgcc ggcccatccg ctggctcttt ccctgattcg cgcggccggc
     7741 gtgccgatcg ctgcgccgag cgccaaccgt tttgcgaaac tgtctccgac aaccgcgcga
     7801 cacgtgcggg atgcgctcgg cggccgcgtc gacctgatcc tcgatggagg acccactgag
     7861 gtcggcattg aatctacggt gctgtccctg gcggcgagcc cgccacggct gctgcgtccc
     7921 gggagggtca cgcagacgga catcgaagcc gtcatcggct cgatcgaaat cgccggccgc
     7981 atcgagaatg gggacaacga tggccatccc tcgcccggcc tgcatccgaa acactacagt
     8041 ccggccacgc cgcttgttct tgtggatggc gggcctcttc ccgccggccg gggcgtctat
     8101 ctctaccgcg agcgccacgc gcaggctgcc cgttgcgtcg caatgcccac ggacgctggc
     8161 gcctatgctg cggttctcta cgagacgctt caccgggagg acgcggccgg gtgggactgg
     8221 atcgccgtcg agcggccgcc cgaaggaccg gaatgggccg gtgtcaccga ccggctcacg
     8281 cgtgcggagt cgaaacgatc cggaagcggg gacaagtcat gatccagcct gtccggaagg
     8341 gccgtgagtt gctcgatgag atcgacaacg aagctgccca aacgcctgtt ctgtggtggc
     8401 ttgggcagag cggctttgtc gtcaagtacc gccggatcgt cttctacctc gatccgtacc
     8461 tttccgaatc gctgacgaag aagtacggga acacgaaccg gccgcatatc cggatgaccg
     8521 aggccccatt ccgcggcgaa gacgtcattc acgctgatct cgtcttctcg acgcacgggc
     8581 acagcgacca tctcgacccg ggcaccgtcc cggcgattct cgcctcttct ccggaggccc
     8641 ggctcgtgct gccgaaggcg ctggtcgaga aagcctgtgc catgggagtt ccgagcgagc
     8701 gcatgcgacc aacggacgcg ggtgacgtga tcgagtttcg caaggacgga gcggtcgcga
     8761 cgattcaccc cattccgtcc gcgcacgagc aactggactg gaccccggaa agcggctatc
     8821 cgtacctggg atatgtcatc cggttcggcg agtggacgat ctaccagccc ggcgactgcg
     8881 ttccctacga cgtccttgtg gaacgcttgc ggcccttcgg tgtcaccgtg gcgctgctgc
     8941 cggtcaatgg gcgcgacccg gcccgcggag tcgccggcaa cttcagcatc tctgaagccg
     9001 cccaactcgc cgaagacatc ggcgcgcgct ggctcatccc cgcccactac gatatgttca
     9061 cgtttaatac cgtggccatc ggcctgttcg tcgaacacat gaagatccgc cccagccaga
     9121 acttcaagat cctccgctgc ggcgaacgct gggtggtgcc gaggtagcgc tttcgtttcg
     9181 ctcctggaga actcaagtca ctccgatgag gcgaacaccc tcggcctgag ccgccttcgc
     9241 taacgtcgtg tcaagtgtcg cgagcggcaa gccttcccgc agcgcaagct ccaggtaggc
     9301 ggcatcgtag actgacaact ggtatgaccg ggcaagtcga aggacttcaa gctcgtccgg
     9361 tgagcgatcc accctcagtg gcaatcgcga gaattctcgt aagaaggctg ttgtatcggc
     9421 ttggccgagc cgtttgcggc gttcgctaac caccaagatg tttcgcagct cgaaccatag
     9481 aaggctcggc gcgactgcct cgtcgcttcg gaagtgctca aacgccaggt ccgcagcggg
     9541 atgatcctca tcctggaacg cccacgatgc ggccacggat gcatctagga cgaatggcat
     9601 cagccttcgc gaccctcgtc tctggccgac agcagttcat ccgacgtgat tcgccctgtg
     9661 cgcttgcgaa ggttggcgat tctcgcaagt gcgtcgtcga tttccctctg gcggcgattg
     9721 gcctcgggaa cgattctggc gatggggcgg ccgtgccgag tgatgacgac cgtctccccg
     9781 cgctccacag cgtcgaggag ccgtggcaga tgagttttgg cgtcagaagc ctgaatctcg
     9841 cgcataaaat tcaaccagtc gattcgacta gtctaatttt acaacggaga cgggcagccc
     9901 gtcaacccga ttccgataca aggccagcaa cccgctaatc cctcagggtt cacctgggag
     9961 aagacgtcga ctcccgggaa ttccagcaat tccgccagat cgggcgcgga gtctcgactt
    10021 gttcggctgt tggacagtag aatcagggtg ttgcggtcac atgaggaacg acagcgaagc
    10081 tcttcctgaa ctgatgatgc ctacgagcgc gatgcgcatt ttttcggctt ggtgcgagtc
    10141 aagcgtgcgg gacaggttgc cgccttcgag tttggagttt ctgccgaggg atattctgcg
    10201 ctcaggcgca tgttcaatgc gcgtcccttc gatgccatgc cgggcctgaa gaacaggtac
    10261 ttctttaccg gaacctacgg acgacggaaa ctaggcgaaa agcctgcttt cttccaggtg
    10321 cgcatcgagc agggctggac tgcgaaattg ttcgagtttc cagggccaac ctcgttggtt
    10381 gcgaacctac tctggttcaa agaaatgaag accttggaag ctgcatctgc cctgaagcgc
    10441 gtggatacgg cgctctgaag cggagctacc ttctggtttc gaatcgcatg tcgaggccga
    10501 tatgacccag tgggcgctct tgaagattaa gtccatgctc catttgggac gtgcagccat
    10561 cttctcaccc ttcccgtcat ggcaaaatga aaggccatgg ccaaaatcca gcgcgcgctc
    10621 atcagtctca ccgataaaag cggaatcctc gaatttgctc gcggattggc ggagctcggc
    10681 gtcgagatcc tctccacggg cggaacggcc cgcatgctcc gcgacgccgg acttgcggtc
    10741 cgcgacgtcg ccgatgtgac cggctttccg gagatgctcg atggccgcgt caagacgatt
    10801 cacccgcgca tcgccggcgg catcctcgcc attcgctcca atgaagaaca tatgcaggct
    10861 ctcgccgcgc acggcattcc caccatcgac atggtcgtgg tcaacctcta cgcattcgag
    10921 aaagttgccg ctcgggaggg cgcgaccgtg ccggagctga tcgagaacat cgacatcggc
    10981 gggccgacca tgatccgcgc cgcggcgaag aactaccagg acgtcgccgt cgtggtttcc
    11041 cccgccgact acgatccaat tctcacggag ttgcgagcct ccggcggctt gtcgctggcg
    11101 acgcactggc gccttgcgaa aacggcctac cgcctgaccg ccgactacga ccgggctgtg
    11161 agcgcccggt tggcgcggat cgaggtccgg gaaggcgcct tcgctgacag cactccaaac
    11221 ctgccgccgg tgctcgactt gcgcgccgat cttgcgctcc cgcttcgcta tggcgagaac
    11281 ccccaccagt cggcggcgct gtactcggac cggaggagcg gcatcgccgg agccgagcag
    11341 ttgcagggta aggaactctc ctacaacaat ctcgtggacc tggacgccgc atggcaactc
    11401 gtctccgagt ttggcgatcc tgcctccgcc atcatcaaac acacgaatcc ttgcggctgc
    11461 gccgaggcgg cctcgctcgc ggagagctat cgcaaggcgt tggagtgcga tccggtgtcg
    11521 gcgttcgggg gagtgctggc gttcaatcgc ccgctggacg aggagacggc ccgcgagatc
    11581 gccaaaacct tcatcgaagc catcgccgca cccgattact cgcaggaagc actggccgtg
    11641 ctggccgcga agaagaacct gaggcttctg cacgtgacga atccagcgcc cgatcccgtc
    11701 gtgatcaagt ccatcagcgg cggcttcctt gcccagactg ccgacctgca tcgacttgac
    11761 cgggcgcaga ttcaggtgaa gacgaaacgc gttccaaccg aagaagaatg ggcggctctg
    11821 gagtttgggt ggaaggtggt gaagcacgtc aagtcgaacg ccatcgtgtt tgccagggcg
    11881 ggacagatcg ctggcgtggg cgccggccag atgagccgtg tggattccgt caaggtagcc
    11941 gccatgaagg cggtgttgcc gcttcagggg accgcactgg cttccgacgc cttcttcccg
    12001 ttccccgacg ggatcgagga agctgcgcgc tatggcgcga cggcggtgat ccagccggga
    12061 ggttcggtga aggacgccga agtgatcgcc gcggcgaacc gtctcgacct ggccatggta
    12121 ttcaccggca ttcggcattt ccggcattaa gtgaactgga gaaccccaca tgagacgaga
    12181 gtttcttagc cgccgccgcc tgctgaaggc aagcatcctg gctgccgccg gagctgccgc
    12241 gcagacgcag ggaaaggcta ccgaattcca gattgcatgc atgacgctcc cgtatgccgc
    12301 ctttccactc gaacgggcgt taaaggggat cgcgggcgcc ggctatcgct acgtcgcctg
    12361 gggcgccacc cacaaggagt ccgacgggca gaccagacag gcactggccg tggatgatcc
    12421 cccgtccgcc gcgaaggagc ttgggagtcg ttcgcgcgac ctcgggctcg aaccggtgat
    12481 gatgttctcg ctggtcaacg tggaggcgcc ggactcgatc aagagccaca cccgccgtat
    12541 cgagcaggcg gcggcagcgg gcgttccctt cgtgctgacc ttcggccata cccggatcgc
    12601 ttcctatgag acgtggatcc ggaacctgaa ggaactcggg ccgatcgcgc ggaacgccgg
    12661 ggtaacgatt gtaattaagc agcacggcgg caacagcgcc accgggaagc tctgcgccca
    12721 gatcctgcgg gaagtcttcg acgagggcat ccagatgtgc tacgacgccg gcaacaacct
    12781 cggctacgta ggcgacgacc cgatcgcgga catccgccac tgctggcagt acgtgcgcgc
    12841 ctttgcgatc aaagacgttc gcctcacgcc gaaacgggac gcttgcggtc ccggccttgg
    12901 cgagatcgat cactacaagc tgctgctgcc cgtggcgcgg accggcctca agattccgct
    12961 ctgttgtgag acgctctggg cgccgctgtt gccccgcccg gagcaaccgg aaggcgtcga
    13021 cgccctggcc aaacgggccc gcgaattcct ggaggttgta acggtcggag tgacagcctg
    13081 acccggaccg cgaagtgggc tacttgtttt tcggtgtatc cggtacgccg gagtcgaaga
    13141 caactttcca cgaaccgtcc gcctgctttt tccagatagt gacgtactta ccggtcgcca
    13201 ccacgccggc gttctcgccg ctgcgcacaa tctcgtacgc gccaatcgtg tagccaagat
    13261 cgccggaact ggaaatcgct gcatcgagcg gctgccaccg gatcgacatc gcagggttgc
    13321 tcagaagaga cgcccagcgt tcggcgaccg ccttccttcc gcgaatgacc ggcaaatccg
    13381 gccgcaacgt cgcggtgtct tcggcaagga atgataagaa gccctccaac cttcctttct
    13441 cagtcgctac agcaaacgcc cggtcggcgt ccagcaagac ggaggcgtct gctgtgccgg
    13501 cgaaacccgt tggggcgatg aggagcgcga gcagaagtgc cgttgttgtt ggcatcggat
    13561 tattcgcccg ttctcttcac agacctcgcc ggcatcgccc agtcggccgg aacctgaccc
    13621 tcaggcaaca actccgcctt ctcgagatac cggattttga ccgcgaagtg cgcggcgtgc
    13681 gggtcatgcg tcaccatgac gattgtcttg ccgtattcct tgttcagctt tgaaagcagc
    13741 gagagcactt cctgcgcgga accagcgtcc aggtttccgg tcggctcgtc ggcaaggatc
    13801 aggtccgggt cggtgacgat cgcgcgggcg atggccacgc gctgttcctg tccgccggaa
    13861 agctggcgtg gcaggtggtg aaggcggtcc ccgaggccca ccagtttcaa cgccgtcgtg
    13921 gcgtgcgcga tccgctcgcg cttcttcagg tgcgtcagct tcagcggcag ttccacgttc
    13981 tcgaacgcag tcagaacggg aatcaggttg aacgtctgaa agacgaaccc gatgtgcgtg
    14041 ttgcgccaat ccgcgatctc accgtcgctc aggtcgcgca gattttctcc caggacccgg
    14101 atctcaccgc cggtcggccg gtccatggcc gcgatcaggt gcaacagcgt ggactttccc
    14161 gagccggacg gccccatcag cgcgacgaac tcgcccttgt gaaccgtcag attgatcatt
    14221 ctcagcgcgg tgatgccgaa gccatcccgg tggaattcct tctccaggtc tcgcgtttcg
    14281 atgaccagtt cgctcatgcc gtcaaccttt cctgatccgc acgcggtcgc cgtctttgag
    14341 ctccgatggc ggattcacga tcacatcctc gcccccggtc aatccctgct cgatgcgaat
    14401 cccgtcgttt gtggtcgcgc cggttcgcac cgcccggcgc acggccttct ctccgagggt
    14461 cacaaagacc gcgccatcgc ggacagccga cgcgggaatc acgatgctgg gccgcaccgg
    14521 cgcgccggtg gccccttctt catagaacgc cacgctcgca ttcatctccg gccgaaggta
    14581 ctcgtcggga tgaagtattt tcaccttcac ctgcacggtg gccttctgcc ggtttgcttc
    14641 cggggagatc tcgtcgatca cgccctcgta cttccggtct gggaacgcat cggtcgtcac
    14701 aacgccgcgt tgctgtgcac cgagcttggc gaaatcgttc tggctgatgt caagttcaac
    14761 ttcgagatcg ttcaggtccg ccagggaaac tacgtaaccc tttgccccgc gctcgcccac
    14821 gaaactggtc gtgacgaact cgcccttttc gaccacccgc tccaggatgg tccccgtcac
    14881 cggcgcccga atcacggtat tcgcaagctg cgtttcgtaa aaagccacca tgccgcgggc
    14941 ctgctccacc tgccctcgca tggcgtcgat ctgttcctgc cgtggcccga tgcgaacgag
    15001 ttcatagctc ttctcgagcg atgcaacgcg tgccgcttgg gcgtcgtacc gcgcttgggc
    15061 atcgtcgagc gactgctgcg atgttacctt ctccgcgtgc agcttacgaa tgcgatcgag
    15121 attcgtcctg gcgttctcga ggtcggctcg cgactgctcc agattcgcct tcgcgaccgc
    15181 aacttcctcc ggccgggaac cgttcagcag ttcctgtagc cgtgcctcga gattggtgag
    15241 ctggccgcgt gcctgctgca actgcgcccg gtattcgtca tcttccagcc gtacaatgac
    15301 ctgcctctct ttgaccttgt cacccttgtc cacgccgatc caggccactc ggccgatgac
    15361 cttcgaggcc acctcgatct tgtgtgccgc cacgatatag cccgtggcgt tcaggatcgt
    15421 gttccggccg ttgtccccgg ccccgccgga ggccgaaact ctcaccaccg agacttcggt
    15481 ggctgcgttc atacggctgt atagaaaccg tcccagcccg agtagcagga aaaacgcgac
    15541 gccggcgaca atccatcgcg tcgcccactt aggctcgcct ttgtcgcggt gcttgcgctc
    15601 gatctgcaag cttttcaggt ctgcatccat ctcgatctct tagatttcac gcagtgcgat
    15661 gaggatctcc ttcttcgctg ccatcagggc ggggaacaag ccgcccacgg cccccatcag
    15721 catggcgaag atgagccctg tcgtgattgt ctttggtccg acgtggaaat tgaaagcgat
    15781 ctcgctgaag gtcacgaaat tcccgatccc ggtcgtgacg ccgttcaggg gcagtgtcag
    15841 gagcactccg ataagcccac cgagtaatgc caacagcacc gactcgatca gaaagctgaa
    15901 caggatgctg ccccgggaga atccaaggac gcgcaacgtg ccgatctccc gtgcgcggcg
    15961 ggccaccgcc gcatacatgg tgttcatggc tgcgacgctc gacccgaccg ccatgatgat
    16021 cgccaccagc gttccaatgt actggatcgg cgcacccgaa accgtctgcg ccgcgtaata
    16081 gtccttctcc gtccgcgctt cgacgttcaa gcgccggtcg ctgttcaggt cgttgatgag
    16141 cgcctgcgcg gccacctcgt cggacgcttc gaccagcgcc gagctcacca cctcggtgcg
    16201 gttgtagtcc gagctcaact ggttcagatc cgcgaaaatc tcgctgttgg agacgcccgt
    16261 tcccgcctcc attacgccca caacgtccca gtcgccgctg ccggcgtgga ttttcttccc
    16321 gacctccgct ccaggaaagc gtttggcgat cgatgagccg acgacaacct cgcgcctgcc
    16381 gctctcgaac catcggcccc ggagcagctt caggtcctcc cgcatctcta tgccgacagg
    16441 catgagcccc cgaatcgtga cgttcatccc ctccgggctg tcgacgcggg ggaggttcac
    16501 caccgtcacc atttccaggg aagccctggg ctccccggac ttgcctcgtt ggatccccgg
    16561 cttcgcccgg accacctgat acatttcacg ggtgaaattg ctgaccagct cggattccga
    16621 tcccttccgc aggatcagca catgcaacgg gttgcctgtc gactggaagg cgatccttag
    16681 gccgtccagc agcgccagaa ccgagagcag caccgcaacc gtaagggcga cgccaagcgc
    16741 cgtcatgacg gtggtcgtct tgcggaccac caggttccgc agattatatg tcaacggaat
    16801 cttcatgttg tccctctgtc tgctaacctg cgtgccgcag ggcgtccacg atcgacgttc
    16861 tggatgcgct ccaggccgga actgccgcgc tcaccagccc cacgaacact gcgattgaca
    16921 gcgatacagc caccaccggc gggccgacgg ccaggttcct taacatcgcg agtgccgccg
    16981 gcccgttcct gatggcgctg actagccccg ccgccagcag gcacccgagc acgccgccgg
    17041 ccagcgagat gaccgtcgcc tcgccgagga taatccccac gatgccgcca ggcttgaacc
    17101 ccagcgtctt catcacgccc acttcccgga ttcgctcgcg gaccgacatc gccatggtgt
    17161 tggcgctcac cagcaggata gtgaacgtga ccgccgcgaa gatgctcagc aggaacgcct
    17221 tcacgttgcc gaggaaagcc agaaagctca gcgcgaaagc ctgctccgtc tccgtccggg
    17281 tctggaacgg cgcattacgg aaggtttcgt cgaccgctgc cgcaattcca ccgacggcgt
    17341 cgggactggc cgcgagaatg gtaaaggtcc cgaccatccc ctgccgcctg ttggggagcc
    17401 cttcctccag gtatttccaa tggaaataca gcacttcgtc gtcttgcgcg gcttcccaga
    17461 tggcccggac cgtcaactcc aggtcgacag gaaagatgtc gcccttcagc gtgatgcggt
    17521 cgccgagttt caggttgtgc ctctcggcca gcggccgtcc gatggcgcag gcagtgcgct
    17581 ccgccagaaa cgcccgcttt tggtcctccg gcaccctgat ttcgccgcga accgtgaaca
    17641 gcttctcggg ttcgacggcg aaccgggcga aaaaattctc cggatcgcgg ctatcccgaa
    17701 agacgcctcc gaaccattgc gaaatcatga cttcctgaac accggggatc tgccggatcc
    17761 gctcgcggta gtagatcggc atcggcaccg ccagcgaaac acggttacgc acgatgagac
    17821 gccgtgcctg ttcgggggat gcctccgtca ggtagaaggc ctgatagatc gccatcagca
    17881 atccgagcag acacagcgac actcccaggc tcagcacggt cagtgccgtg cgacggcggt
    17941 tccgccaggc gttcttaaag ataaacggga ggtagcgcac agggtattag attcatctta
    18001 gaatagctgc ggcgcccggt ggagggcctg tctgccgcat gaccgacgaa acatggatgc
    18061 tcgaggcgct tgcccttgcg cgcgaagccg gaagtgaggg tgaggtgccg gtgggagcca
    18121 tcgtcgtctg cggcggcgtg atcgtcggac gcggccggaa ctcgccactt gcccgggtcg
    18181 atcccaccgc tcacgcggag atccttgctc tccgggaagc cgcggcacgg ttggagaact
    18241 accggctgga ggactgcacc ctgtacgtga cgatcgagcc gtgcgtcatg tgcgccggcg
    18301 cgctcgtgac ggcgcgcgtc cgccgtctcg tgttcggcgc cagggacttg cgcttcggcg
    18361 gcgtccgcag caagttccga gtcgccgact ccgacctgct caatcaccgc gtcgacgtgg
    18421 aggaaggcgt gctcgcgacc gattgtgccg agttgttgcg cgaattcttc cgggaacgcc
    18481 ggtaatcggc ttcgcaggat cggagcccgg ccggggaccg ccgtccgcgc cattccggct
    18541 cggacggcag ctcccggcag cgcgctgccc tgtactcacg acgaggagtg aaggcgggag
    18601 tcaggcagtt ttcagtaaag gcggatgaaa gacgggccgc gccgcacgca atggcgcggc
    18661 gagggcggat gacgcagggc ctttgcgatc gggctccgta agcatcgttc cgtggaaata
    18721 atcgtgcagc ggatagagcg ggtacggctc tagttcgcgg aatgcccggc cgagcgtttg
    18781 ctcgatccga tagacggaat ggaagaaatt cccacggtcc atcttgagct tccggcagca
    18841 gagcttccag tctgctccga gaagaaagtg ataccggaag accatatgtt cgtactcggt
    18901 gagcatccgc cggctcacca gacagaaatc ggctacgtac tcctcatctt tgcgtcccca
    18961 ggaccatctc ccctcccgcc cggagatctt ttcgagagaa acccggctca agtacttttc
    19021 tttcgcggca cagtagcgaa accgcgcgta gcaggcgcgg aaaatggcgc gaaggacgca
    19081 gttgcagggg tacatccgct cccggtgccc ctgcctcaat cccaggccat ggcagtgcga
    19141 acatgagtgt ctcgccattg ctaacgtttc ggatctggtc cattccataa ggctcattcg
    19201 cggcggcgcc ctttgggacg cctttccgct cctgctcaca gactattcca ggtccgaaaa
    19261 tcgtcaacat taaattcgca gtattttccg ggaaatatct taaatcgatg taaattctat
    19321 tttatcattt gttttcagcg tcttacggcg atctcaattt gcggctacgg tactgaagtt
    19381 atgagagttc tatgatagta tttcttaaga agtactacct atttcacagg gaaatagggc
    19441 gtgggcccca caatggactt ccaggaactg caggcgcgcc tcgtcgccgc tctcaaagcg
    19501 cgcctccaca acggagagct cacggaacgc cggctcgcac gcctggccgg gatttcgcag
    19561 ccgcatatgc ataatgtgct gaaaggcgca agaattctct ctcccgacgc cgccgatcgc
    19621 gtcctcaaat gtctccgcct gtcagtgcta gacctgatca ccgtccccga actccaccag
    19681 gcgggctgtc cccagtgccc gaaacatggc gcctaccggg gagtcccaat tctggagggt
    19741 tggctgggac ccgggctgcc gcttcccact gccaccggcc cggagtacag atgccccttc
    19801 ccgaccctgc ttgtcacctc gctgaccgat cccgtggtgg cgcggctgac cgcggatgcc
    19861 aggatgaccg cggtggtccg ccacaatgat ctcgtcctgc ttgatagatc ccaccacagc
    19921 cggtcccggc tcgacggaga gggcttatac gtagtaaacc gtcacgggga aggactcatc
    19981 cggcggctgc gtatggaagg ccggcgcgtg tttcttggaa ccgaagccgg cctcggacgg
    20041 ccgtgcgaat gggaggagat tgtgctggag aacagccacg tcatggatat cgttcgggcg
    20101 cgtgtcgtct ggatcggacg gtatcttcta agcttctgaa acgcgccggc gatccggcgg
    20161 agcccgcgct cctcagcgca gcacctttcg ttctcgtccc acttcttcgt acacttccag
    20221 cacttgccgc gccgttgact cccaactgaa acgccgcgct tgctcgtagc cccgggcaat
    20281 cagcgagacc cggagctgct catccgtcag cacgtctcga ataccccggg cgatgtcgaa
    20341 cacattctcc ggattcacca acgccgcggc gtcgccgaca acctccggta gggaactgcg
    20401 attcgacgac acaaccggcg tcccggaggc catggcttcc agcggcggga gcccgaagcc
    20461 ctcgtacatc gatggaaaaa cgaacgcagc agccgcttca tagaagacgc gaagagtgtc
    20521 gatcggaacg aaacccagaa agcggacgac gtcctccacc cgcgtctgga tcaccgcccg
    20581 ccggaccgcc ggataccggc tcagttcatc gccgatgatg atcagccgga gatccttata
    20641 caccggatgt gtctctagct gcccgcggac tacggcgaac gcctccacca gccggggcac
    20701 gttcttctgc gggcggatcg atcccgtgta cagcacgaac gggtagtcga tctgatagcg
    20761 ctccaggatg cggcgcactt cgcggctgtg accctcgggg ccggccgagc gcgcgtcggc
    20821 ggcgggcttg tgctccagga agcgggggtc cggcgcgttg taaatccgcc gcaccctctc
    20881 cggcagaatt ccgaagaggt cttccacgtc tcgcagggtg gcctccgaaa ccgcgatgac
    20941 cttttccgcg cgctgaagac cccggcggaa ccggtacagg cgcaacctcc gccaaaagtc
    21001 gcgctgccgg tcatacatca ggctgctcat gtcgtggatc gtgaccacgt acggctgggg
    21061 catcgccagc gacaccgcat tcagcgggat gtgatagaga tccgccgaga agtggcggag
    21121 gaaccacggg aacgcgacgt catccagcgg gtcggaatca tggcgctcat atctggcgat
    21181 cttgaaattg ccgggaaggc ccgccagctc tgcgccgcct tccttcccgc aggccagcac
    21241 atagtggttc tcccggtcga tgcacgccag agcgcgtacc aggttgcgta tgtacgtgcc
    21301 gatgccgaag tcccgcaggt gccgggcgtc gatcagtatg cgcagcgcca ttcaattcgc
    21361 ggcgacggcg ttaagtttcc agtggtacag cggaaaccgt ccggtcaact ccgccacccg
    21421 gcggcggacc gaagcgatca catcgtcgtt tccgaggttt ccgagcacct cggcgatcat
    21481 cgacgcgacc tcgcgcattt ccggctcgcg gaacccccgg gtcgtcactg cgggagatcc
    21541 taaccggatc ccgctcgggt tcagcggcgg gttggcgtcg aacggaatcg cgttcttgtt
    21601 gaccgtgatg tgcgccttgt cgagccccgt ttccgcgtcg cggccccgaa ttgcccgtgc
    21661 aaacacgtcg accagtaaca ggtgcgtgtc tgtgccgccg gagaccaccc ggtaacccgc
    21721 ttccgccatc ccgtccgcca gcgctgccgc gttcgccttc acctgcctct gatagctcac
    21781 gaactcgggt gtcatcgcct cggcaaagca taccgccttg gctgccacca cgtgtaccag
    21841 cggccctccc tgcacccctg gaaacacggt cttgtcgatc gcctgggcga actgtgcgcg
    21901 cgacaggatg atccccgcgc gcggcccgcg cagcgtcttg tgagtcgttg acgtcacgat
    21961 gtcggcgtat tcgcagggat tcggatgcag accggccgcc accagtcccg agaaatgagc
    22021 catgtccacg aggaagatcg ctccggcggc gtccgcaatc tcgcgaaatc gccggaaatc
    22081 gatgatccgc ggatacgcgc ttccgccggc gataatcatc ttcggcttgt gctcttcggc
    22141 cagcctcgcc agctcgtcgt aatcgatgat ctcgtcttcg cgccgcacgc cgtacgggac
    22201 caccttgtag gtcttgccgg agaaattcag gtggtgcccg tgcgtcaggt gcccgccgtg
    22261 cgccaggttc atgccgagga tcgtgtctcc cggctggagc accgc
//
LOCUS       DATIFQ010000138         9906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_431059, whole genome shotgun sequence.
ACCESSION   DATIFQ010000138 DATIFQ010000000
VERSION     DATIFQ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431059"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VLE22_06350"
     CDS             <1..339
                     /locus_tag="VLE22_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14060.1"
                     /translation="SELSAPLRMLYPSGSVDSPAFLSGDAKTQAEAERKRMASLPTAP
                     NHLAGQAIRWALVNPQDPRVPEALHLAVRATRYGCVDAATSRFSRQAFELLHRRYPKN
                     VWARKTRYWF"
     gene            complement(349..1395)
                     /gene="recA"
                     /locus_tag="VLE22_06355"
     CDS             complement(349..1395)
                     /gene="recA"
                     /locus_tag="VLE22_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HSB14061.1"
                     /translation="MDEKERRRVIDLALVQMEKQFGKGAVLRLGSGNVVPVEVIPSGS
                     ISLDAALGVGGFPKGRVVEVFGPESSGKTTIALQVIAEAQKAGGTAAFIDAEHALDAA
                     YARKLGVDVDNLLVSQPDFGEQALEITSALVSSGGIDVAVVDSVAALVPKAELDGEMG
                     DNHVGLQARLMSQALRKLTGTVARNNACLVFINQIREKIGVMFGSPETTTGGRALKFY
                     SSVRLDVRRISTLKEGDKVVGSRTRVKVVKNKVAAPFREAEVDLLYGEGISRDADLLD
                     LATTHNLVEKSGSWFSYKGERIGQGRENARMFLKEHSDTRDRLEVDVRRLLGLPLNGL
                     ASLTTSVPVATASR"
     gene            1458..4700
                     /locus_tag="VLE22_06360"
     CDS             1458..4700
                     /locus_tag="VLE22_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSB14062.1"
                     /translation="MGLIHSFRRLILIAFAVPLLAAPSDRTAGRYALILEDPPAAEVA
                     AQSKGITPRAAEDHSRRIEAAQSTLRKNLEQLGIQPTGAVQLLLNAVFVRCTPERIPE
                     LSSLPGVKGVVPLPDLELKLNAAVPAANGPAAWNLLGGVGSAGAGVKIAVIDTGIDYQ
                     HPAFQDDSLPMPAGYPKCDGSDCAFTNHKVIAARSYVRLVAAGTGPDPAADSRPDDLS
                     PRDRVGHGTAVATAAAGWTSTGPLGTITGMAPKAYLGNYKVFGSPGVNDGTSGAAVIS
                     ALEDARKDGMDIAVLSLGGSAFTGPLDEGAACGAPPDVPCDLLARAVENAVQAGLTVV
                     VAAGNEGNDGEKSPTLNSVSSPATAPSAIAVGAVSSPRTFTSSVRVAGGAVPENLRVI
                     SAVFGDGPALSSTLTAPLRDVTEFGADGLACSVKIPLESLRGALVLIQRGSCLFLEKL
                     LNAQQAGAVGVVFYQQDGSEDLIRPGGLTGTSIPAVMIGNTAGRALKQYLASNRGAPV
                     TLDPSLIAVSGGAPNTMAAFSSRGPSAGESAIKPEIVATGTTMYMGTQKFDPNGVMYD
                     ASGFTVAQGTSFSTPLVAGAVALVKQKNPDFSPADLKSAVANTATADATDGGVPVGAV
                     AQGGGRLDARTAVQAGFTAEPSTLSFGVLRSGSLPRTQGLTLHNRGTAQLDLTLSVTS
                     SGLLSLDQTVVTIPPGRSVEVLATLSGTLPPAGSYEGAVSVQGGGTTLHVPYLYLVGD
                     GVAYNLLVLSGSGFVEAVGSSIPGHGLSFKLTDQYGAPVQGAPVQFSVVRGGGAILAA
                     NSQTDAYGVARAQAVLGPVAGIQQFSAVSGGLTAMFSGGARVRPTIQTNGIVNAASFK
                     AGAAPGSYVSIFGSGLADEILSTPSTSLPLSLSDVSVSFDVPSASLSLPGRLHLVAPG
                     QINVQVPWELHGQTSVLVKVSIGDVSSEVYTLPLAEYSPGVFEYADPVSKQTLAAALD
                     EKNAVVSGGNPVAKGRPVQLFLNGLGPVDHTQETGEPAPADPLARTTVMPEVTIGGVK
                     AVVEYSGLAPLFAGLYQVNVVVPGDAPTGIQPLVVTVNGVTAQASRLPVQ"
     gene            4729..5745
                     /locus_tag="VLE22_06365"
     CDS             4729..5745
                     /locus_tag="VLE22_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSB14063.1"
                     /translation="MFAGLQTAVMWTLLGLLVLLLYLIVKPFLVPLAWAVILVVFLYP
                     LHWRLLKRVRRPSLAALASVLAVTVVLIVPVSLLVPVFIGETVSFVGALTTAELFRKA
                     STWLDSYIQTLPLEIPSTGELIQTWGQDLIGVAATQSAKIAGNVATTLFGLVMMLLAM
                     FYLFRDGPALVGFLEDVSPLSNERQRRILDEVTEMIRVTMTSSFVVAAIQGASGGLVY
                     WILGLPSPLFWGALMALLSLLPVLGAWLVWGPMAVVLLFSGHTAKGIVLLVLGTLLVS
                     GVDNVLRPALIAGRAQMNGLLIFISLLGGVAAFGFLGIILGPVLVATFVGLLKGYHES
                     LNVT"
     gene            complement(5754..6638)
                     /locus_tag="VLE22_06370"
     CDS             complement(5754..6638)
                     /locus_tag="VLE22_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HSB14064.1"
                     /translation="MRTARLLAVVALLASTAPGFPDSDPAYRASIEEWRRGREARLKA
                     DDGWLTVAGLFWLKEGANSAGSGASNGIVLPREAAPERIGSFVLKNGKTVFLAERGVP
                     VTSRGKTVGTLEMKPDTSGNPDVIAISSLSMHVIQRGKRCGIRLRDKNSRFRREFTRL
                     LWFPVNESYRINARFVPYNPARKIAIPNVLGDIETETSPGYVVFTVDSVECRLEPVSE
                     GDQLWFIFRDLTGAKETYPAGRFLYADPPKDGKVVLDFNKAYNPPCAFTPYATCPLPP
                     PPNRLQVRIPAGEMRYHE"
     gene            complement(6648..7724)
                     /gene="recA"
                     /locus_tag="VLE22_06375"
     CDS             complement(6648..7724)
                     /gene="recA"
                     /locus_tag="VLE22_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HSB14065.1"
                     /translation="MDEKERSRMLGLALSQIEKQFGKGSILRLGAKEAIVPVAAIPTG
                     SISLDAALGVGGFPRGRICEIFGPESSGKTTVALQVIAGAQKAGGMAAFIDVEHALDP
                     IYARKLGVDVDNLLVSQPDYAEQALEITSALITSGSIDVLVVDSVAALVPKSELDGEM
                     GEAQMGLQARLMSQALRKLTGVVSKSNTCLIFINQIREKIGVMFGNPETTTGGRALKF
                     YASIRADIRRIAAIKEGDAITGNRTRVKIVKNKLAPPFREAEFDILYGEGISREGDLL
                     DLGVAHNILEKSGSWFSYKSERIGQGRENARQFLRDNVDICQKVDAELRKILGLVREE
                     KGAAPSPEPQPQSKPVPASASRGR"
     gene            complement(7803..8741)
                     /locus_tag="VLE22_06380"
     CDS             complement(7803..8741)
                     /locus_tag="VLE22_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB14066.1"
                     /translation="MKPFTGILFLACALAAVPAVGQSNQQAGTAVLDEKTRIILDVTR
                     VNLLFTVTDKKGRFVTDLTKDDFAIREGKRPQTILEFVSESDLPLRLAILVDTSNSIR
                     DRFKFIQEAAIEFINSVVRPNQDKAAMISFDTAAELQADLTGDLATLDKVIRNLRPGG
                     GTALYDAIYFACRDKLMMDQPRHKFRRAMVILSDGEDNQSRYSRDQALEMAQKADVVI
                     YTISTNITRVETDGDKVLKYLSEETGGVTFFPFKVEDLSQSFENIANELRHQYNLLYR
                     PEPMKTDGTYQTVDVRVKERKGLIVRARKGYYSPRL"
     gene            complement(8759..9667)
                     /locus_tag="VLE22_06385"
     CDS             complement(8759..9667)
                     /locus_tag="VLE22_06385"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSB14067.1"
                     /translation="MTFLSQSSDVGKRLDHFLQEQLPSYSRTRLQDWIRSGRVRVDER
                     QEKPSYCLRGGEKVDVDPAELRRLKAEPEPIALQILYEDNDVVAVDKPAGMVVHAGAG
                     CRSGTLVNALLHRFGELSGLGGELRPGIVHRLDRHTSGVMLVARNDAAHRRLAEQFAR
                     REVEKVYIALVHGVLKTDRGRIEKPITRDPARRIRMTARLGRGRTALTEYRVVRRYHA
                     FTLLEVKIGTGRTHQIRAHLASIGHPVAGDRLYGAPSRVEGQPSLDRFFLHSHRIRFE
                     QPATRQTITLESPLPPELEGWLEGLL"
BASE COUNT         1714 a         3116 c         3089 g         1987 t
ORIGIN      
        1 tccgagctga gtgcgcctct ccgaatgctc tacccgtcag gctctgtgga ctcccccgcg
       61 ttcctgagtg gggacgcgaa gacgcaggcg gaagcggagc ggaagcgaat ggcgtccctc
      121 cccaccgcgc cgaaccacct cgcgggtcag gcgatccgct gggcgctcgt gaatccgcaa
      181 gaccctcgcg ttcccgaggc actccatctg gcagttcgcg ccacccgata cgggtgcgtt
      241 gatgccgcga ccagccggtt ctcacgccag gctttcgagc tgttacaccg ccgctaccct
      301 aagaacgtct gggcgcgaaa gacgaggtac tggttctgag aggctccctt accgactagc
      361 tgtggctacc ggaacggaag tagtgaggct tgccaatccg ttcaagggca acccgaggag
      421 tctccgcaca tcgacctcaa ggcggtcccg ggtgtcagag tgttccttca ggaacatacg
      481 tgcgttctcg cgaccctggc cgatccgctc tcccttgtaa ctgaaccacg agccgctctt
      541 ctcgacgaga ttgtgcgtgg tggccaggtc caacaggtcg gcatcgcgcg agattccttc
      601 gccgtaaagc agatccactt ccgcctcccg gaacggggct gccaccttgt ttttcacgac
      661 cttgaccctc gtgcggctgc caacaacctt gtctccctcc ttcaaggtgg aaatccggcg
      721 gacatcgagc cggacggagc tgtagaactt gagcgcccgg cctccagtgg tcgtctccgg
      781 gcttccgaac atcacgccga tcttctcccg aatctggtta atgaaaacga ggcaggcgtt
      841 gttgcgtgct accgtgccgg tgagcttgcg gagcgcctgg gacatgagcc gggcctgaag
      901 gccgacgtgg ttgtcgccca tttccccgtc cagttcggcc ttaggcacca gcgccgccac
      961 cgaatccacc accgcgacgt caattccgcc ggagcttacg agtgcggagg taatctccag
     1021 ggcctgctct ccgaaatccg gttgggacac caggaggttg tcgacatcga ccccgagttt
     1081 cctcgcgtac gccgcgtcca gcgcgtgctc agcgtcgatg aacgcagccg tcccgcccgc
     1141 cttctgcgct tcggcaatca cttgtaacgc gatggttgtc ttgcccgaag attccggccc
     1201 aaagacttcg accaccctgc cttttgggaa accaccgacg cccaaggcag cgtccaaaga
     1261 aattgagccg ctcgggatca cctcgacggg aacaacgttg ccggagccaa gccgcaacac
     1321 ggcgcccttg ccaaactgct tctccatctg cactaaggcg aggtcgatga cacgacggcg
     1381 ttctttttcg tccacgagta ctctgtgtcc cgacgacagg aattttctca ccaagttcta
     1441 tgcgtacaat cgcaaggatg ggcctcatac attccttccg gcgcttgatc ctgatcgctt
     1501 tcgccgtccc ccttctggcg gcaccgtctg acagaaccgc cggccgctac gcactgatcc
     1561 tcgaggaccc gccagcggcc gaggttgccg cacagtccaa gggaatcacg ccgcgtgcag
     1621 ccgaggacca ctcccgcagg atcgaagctg cccaatccac attgcggaag aacctggagc
     1681 agcttgggat ccagccgacc ggcgctgtcc agcttctgct caacgccgtt tttgtgcgct
     1741 gcactccgga acggatccca gagctgagtt ccctgccggg agtgaagggt gtcgtcccgt
     1801 taccggacct ggagttgaag ctgaacgcag ctgttcccgc cgcgaacggg cctgccgcct
     1861 ggaatctgct ggggggcgtc ggcagcgccg gggccggcgt gaagattgcg gtgatcgaca
     1921 cggggatcga ctatcagcac ccggcatttc aggatgactc cctgcccatg ccggccggct
     1981 acccgaaatg cgacggctct gactgcgcct tcactaacca caaggtgatc gctgcccgga
     2041 gctatgttcg cctggttgcg gccgggaccg ggcccgaccc agcagccgat tcccgtcccg
     2101 atgacctatc gccgcgcgac cgcgttggtc acggaactgc ggtggcgaca gcagccgccg
     2161 gatggacgag caccggccca ctcggaacca taaccggcat ggcgccgaaa gcgtacctcg
     2221 gcaactacaa ggtgtttgga tcgcctggcg tcaacgacgg cacgtcaggc gcggcggtga
     2281 tctcggcact cgaagacgcc cgaaaggacg ggatggacat cgcggtcctt tcgctcggag
     2341 gctcggcctt caccggcccg ctcgacgagg gggcggcgtg cggagcgcct ccggacgttc
     2401 catgcgatct tcttgcacgc gcggtggaga acgccgtgca ggcgggattg accgtcgttg
     2461 tggcggcggg caacgagggt aacgacgggg agaagtcgcc cacgctgaac tcggtcagtt
     2521 ctccggcgac ggcgccctcg gcgatcgcgg ttggtgcggt ttcgagcccg cgcacgttta
     2581 cctccagtgt gcgggtggcc ggcggcgcgg tccccgagaa cctccgcgtc atctccgctg
     2641 tcttcggaga cggtccggcg ctctccagca cgctgacggc gccgttgcgg gatgtcaccg
     2701 aattcggtgc tgacgggctg gcttgctcag tcaagattcc tctcgagtcc ttgcggggcg
     2761 ccctggtgct gatccagcgt ggcagttgcc tgtttctgga gaagttgctc aacgctcagc
     2821 aggccggtgc cgtcggcgta gtgttctatc agcaggacgg gtccgaagac cttatccggc
     2881 ccggtggttt gacggggacc tccatccccg ccgtcatgat cggcaacacg gcagggcggg
     2941 cactgaagca atacctggcc tcaaaccgag gcgcccccgt caccttggat ccgtctctca
     3001 ttgcggtgtc gggcggagca ccgaacacga tggcggcgtt ctcgtctcgc gggccgtcgg
     3061 cgggcgaatc ggcgatcaag ccggaaatcg tggccaccgg cacgacgatg tacatgggga
     3121 cgcagaaatt cgacccgaac ggcgtgatgt acgacgcctc cggattcacc gtggcccagg
     3181 gcaccagttt ttcgactcct ctcgttgctg gtgcggttgc gctggtgaag caaaagaacc
     3241 ctgactttag cccggcggac ttgaagtcgg ccgtggcgaa cacggccacg gctgacgcca
     3301 ccgatggcgg tgtgcccgtg ggagccgtcg cgcagggcgg agggcggctg gacgcccgga
     3361 cggccgtgca agccggcttc acggcagaac catcgacgct ctcgtttggg gttctgcggt
     3421 ccggatcgct cccacgaacg caggggctta ccctccacaa ccgtgggacg gctcagctcg
     3481 atctgactct ttcggtgacc tcctcgggac tcctgagtct cgaccagact gtagtgacca
     3541 tcccccccgg cagaagtgta gaggtgctgg ccacgctatc cggcaccctg ccgccggccg
     3601 gttcctatga aggcgccgtc agtgttcagg gcggcggcac gacgttgcac gtgccgtatc
     3661 tgtaccttgt cggtgacggc gtcgcgtaca acctgctcgt actctccggc agcggcttcg
     3721 tcgaggctgt gggcagttcc atccccggac atgggctttc cttcaagcta accgatcaat
     3781 acggtgctcc ggttcagggt gcgccggtgc agttcagtgt cgtgcgggga ggtggtgcaa
     3841 ttctcgcggc gaattcacag accgacgcgt atggtgttgc cagggctcag gcggtccttg
     3901 gcccagtggc tggtattcag caattcagcg ccgtttccgg cgggctgacg gcgatgttca
     3961 gcggtggcgc ccgcgtgcgg ccgacgatcc aaacaaacgg gattgtcaat gccgcgagct
     4021 tcaaagccgg agccgctccg gggtcttatg tgtccatctt cggcagtggg ttggccgacg
     4081 agatactctc gacgccgtcc acctcgcttc ctctgtccct ttcggatgtc agtgtcagct
     4141 tcgatgttcc ctccgcaagc ctcagcctgc cgggacgtct gcatctcgtc gcgcccggcc
     4201 agatcaacgt ccaggtgccg tgggagttgc atggtcagac ttcggttctt gtgaaggtca
     4261 gcatcggcga tgtctccagt gaggtgtaca cactgccgct ggcggagtac tccccgggcg
     4321 tgtttgaata cgcggacccg gtcagcaagc agactctagc tgccgccctc gacgagaaga
     4381 acgccgtggt tagtggtggc aatccggtcg ccaaggggcg tccggttcaa ctgtttctga
     4441 acggcttggg tcccgtcgac cacacgcagg aaaccggtga accggcgccc gccgacccgc
     4501 tggcccgcac caccgtgatg ccggaggtca ctattggcgg ggtgaaggcg gtcgtcgagt
     4561 acagcgggct ggcgccgttg tttgcgggac tgtatcaggt gaatgtggtt gtgccggggg
     4621 atgcacccac gggtatccag ccgctggtgg tcaccgtgaa tggagttacg gcgcaagctt
     4681 ctagattgcc ggtccagtaa gggtatactg acctcacccg aggcgcgtat gtttgccggt
     4741 ctccaaaccg cggtaatgtg gaccctactg ggtctgctgg tgctgctgct ctatctgatc
     4801 gtcaagccgt tcctggttcc gttggcctgg gccgtgattc tggtcgtatt cctgtaccca
     4861 ctgcactggc gcctgttgaa gcgggtccgc cgtccgagtc tcgcggccct tgcctcggtt
     4921 ctcgctgtca cggttgtcct gatcgtcccg gtcagccttc tggttccggt attcattggg
     4981 gagacggtgt ctttcgtcgg agccctcacc accgcggagc tctttcggaa ggcgtcgaca
     5041 tggttggact cctacatcca gacgctcccg ctggagatac cgagcacagg tgaactgatc
     5101 cagacctggg gccaggacct tatcggcgtc gctgctacgc agtcggcgaa gattgcaggt
     5161 aacgtcgcaa ctacgctgtt tggcctggtg atgatgctgc tggcgatgtt ttacctgttt
     5221 cgcgacgggc cggcgctcgt cggcttcctg gaggatgtga gcccgctcag caacgagcgg
     5281 cagaggcgga ttcttgacga ggtcaccgaa atgatccggg tcaccatgac gagttcattc
     5341 gtggtggcgg cgatccaggg agcaagcggc ggactcgtct actggattct cggcctgccc
     5401 tcccccctct tctggggtgc actcatggcg ctgctgtctc tcctgccggt gcttggggcc
     5461 tggctggtgt gggggccaat ggcggtcgtg ctgctgtttt caggacacac cgcgaagggt
     5521 attgtgctgc ttgtgctcgg aacccttctg gtcagcggcg tggacaacgt actcaggcca
     5581 gctctgatcg ccggacgggc gcagatgaac gggctcctga tcttcatcag cctgttgggc
     5641 ggcgtggcgg ccttcggctt cctcggtatc atcctggggc cggtgctggt agccactttt
     5701 gtcgggctgc tgaagggcta ccacgagtcg ctgaacgtca cctgaggcgg cgctcactcg
     5761 tggtaccgca tctcgccggc cgggatgcgc acctgtagcc ggttcggcgg cggcggcaag
     5821 ggacaggtcg catacggcgt gaaggcacag gggggattgt aagccttgtt gaaatcaagc
     5881 acgaccttcc catctttcgg cgggtcagcg tagagaaagc ggccggcagg gtaggtctcc
     5941 ttcgcgccgg tcagatcccg gaaaatgaac cagagctgat cgccctccga gaccggctct
     6001 aacctgcatt ccacgctgtc caccgtgaag accacgtagc cggggctggt ctctgtctct
     6061 atgtccccga ggacatttgg gatggcgatc ttcctggcgg gattgtaggg tacgaaccgg
     6121 gcgttgatgc gatagctctc gttcaccggg aaccaaagga gccgggtgaa ttcccgccgg
     6181 aaccggctgt tcttgtcccg caatcggata ccgcaccgct ttccgcgctg aataacatgc
     6241 atcgagaggc tgctaatggc gatgacatcc ggattacctg aggtatccgg cttcatctcg
     6301 agcgtgccca ccgtcttgcc gcgcgaagtg accgggacac cgcgctcggc gaggaagacc
     6361 gtcttgccgt tcttcaagac aaacgatccg attcgctccg gcgctgcttc gcgaggcaat
     6421 acaatgccgt tggaagctcc ggaacccgcc gaattcgctc cctctttcaa ccagaacagg
     6481 ccggcgaccg taagccagcc gtcgtctgcc ttcagtcgcg cctcccggcc gcgccgccac
     6541 tcctcgatcg acgcccggta ggccggatcg gaatccggaa acccgggcgc ggttgaagcg
     6601 agtagagcaa caacggccag cagacgcgcg gtgcgcattg ggggcggtca gcggcccctg
     6661 gaggcggagg ccgggaccgg cttggactga ggctgaggct ccggagaagg cgctgcgccc
     6721 ttctcctcac ggaccaatcc caggattttg cgaagctcgg catcgacttt ctggcagatg
     6781 tccacgttgt ccctgaggaa ctgccgggcg ttctcccgcc cttgaccgat gcgctcgctc
     6841 ttgtagctga accacgaacc gctcttctca aggatgttgt gggcgacgcc gaggtccagc
     6901 aggtcgcctt cgcgggaaat gccctcgccg tatagaatgt cgaattccgc ctcccggaac
     6961 ggcggagcca gcttgttctt cacaatcttt acccgcgtgc ggttgccggt aattgcgtcg
     7021 ccctccttga tggccgcgat tcggcggatg tcggcgcgaa tggaagcgta aaatttgaga
     7081 gcgcgtccgc cggtggttgt ctctggattg ccaaacatca cgccgatctt ctcgcggatc
     7141 tggttgatga agatcaggca agtgttcgac ttcgaaacca ctccggtgag cttccgcagg
     7201 gcctgcgaca tcaaccgcgc ctgcaacccc atctgcgcct cacccatttc cccgtcaagt
     7261 tcggatttgg gcacgagcgc cgcgacagag tccaccacca gcacgtcgat cgagcctgag
     7321 gtgatcagcg cgctggtgat ttcaagcgcc tgctctgcgt aatcaggctg ggagacgaga
     7381 aggttgtcga catcgactcc gagcttccgg gcataaatgg gatcaagcgc gtgttcgacg
     7441 tcgatgaacg ctgccatccc cccggccttc tgggccccgg caatcacctg gagtgcgacc
     7501 gtcgtcttgc cggaggattc cggcccgaag atttcgcaga tcctgcccct tgggaatccg
     7561 cccactccca gcgccgcatc gagcgagatg gagcctgtcg gaattgcggc gacgggaacg
     7621 atcgcctcct tagccccgag gcggagaatc gagccttttc cgaactgctt ctcgatctgg
     7681 ctcagagcga gccccaacat gcgcgaccgt tctttttcgt ccattgccag acaatcctca
     7741 ggggggagag tgggttgaaa gccgattata caacaggggc ggaagcgtcc ccggagtaag
     7801 gcctacaggc gtggtgaata gtatccctta cgagcgcgca cgatgagtcc cttccgctct
     7861 ttcacccgta cgtcgacggt ctgataagtg ccgtccgtct tcatgggttc tggcctgtag
     7921 agcaagttgt actgatggcg aagttcgttg gcgatgttct cgaaggactg ggacaggtct
     7981 tccaccttga atgggaagaa ggtgaccccg ccagtctcct ccgagaggta cttgagtact
     8041 ttgtccccgt ccgtctcgac gcgtgtaatg ttcgtcgaaa tcgtatagat gacgacgtca
     8101 gccttctgag ccatctccaa cgcttgatcg cggctgtaac ggctctggtt atcctcgccg
     8161 tcgctcagaa tcaccatcgc ccgtcgaaac ttgtggcgcg gttggtccat catgagcttg
     8221 tcgcggcagg cgaaatagat cgcatcgtag agggcagtgc cgcctccagg ccggagattt
     8281 cgaatcacct tgtcgagagt cgcgagatcc cccgtaaggt cggcctgaag ctccgcagcg
     8341 gtatcgaagg agatcatcgc ggctttgtcc tggttggggc gaaccacact attgatgaat
     8401 tcgatggctg cttcctggat aaatttgaaa cggtcacgga tgctgttgct cgtatccacc
     8461 aggatcgcca gccgcaacgg caggtcgctc tcggagacga attcgaggat cgtctgcggc
     8521 cgtttccctt cgcggatggc gaagtcatcc ttggtgagat cggtaacgaa ccggcccttt
     8581 ttgtcagtga cggtgaacaa taggttcaca cgggtcacat cgaggatgat ccgtgtcttc
     8641 tcgtcgagca cggctgtacc ggcctgctgg ttcgactgtc ccactgccgg cacggccgcc
     8701 aatgcgcagg cgagaaaaag aattccggtg aacggcttca tgagaaccct cgccttgatt
     8761 ataggagtcc ctccaaccag ccctcaagct cgggcggcag cggagattcg agggtgatgg
     8821 tctggcgggt cgcgggctgt tcgaaacgga tgcggtgcga gtgcaggaag aaacgatcca
     8881 gcgatggctg cccttcaacg cgcgacggag cgccatagag ccgatcgccg gcaacggggt
     8941 gaccgatgct ggcgaggtgg gccctgatct gatgcgtgcg cccggtgccg atcttcacct
     9001 cgagcaaagt aaatgcgtgg tagcgccgca ccacccggta ttcggtcagg gcggttcggc
     9061 cgcggcccag gcgcgcggtc atccgaattc ggcgcgccgg atctctcgtg atcggtttct
     9121 cgatccgccc gcgatccgtc ttcaagacgc cgtggacgag cgcgatataa accttctcaa
     9181 cttcgcggcg ggcgaactgc tccgccaacc ggcggtgcgc ggcgtcgtta cgtgccacca
     9241 gcataacccc gctggtatgg cggtcgagcc ggtggacgat ccccggccgc aactccccgc
     9301 ccagacccga cagttctccg aatcggtgca atagagcgtt caccagcgta cccgagcggc
     9361 agccggcccc ggcatggact accattcccg ccggcttgtc cactgccaca acgtcgttgt
     9421 cctcgtagag gatctgaagc gcgatcggct ccggttcggc cttgagacgc cgcaactccg
     9481 ccggatcgac atcgactttc tcgccgcccc gcagacaata cgacggtttc tcctggcgtt
     9541 catccacccg cacacggccg ctacgaatcc agtcttgcag ccgcgtccgg ctgtaggacg
     9601 gaagctgctc ctggaggaag tggtcaagcc gttttcctac gtccgatgac tggctcagga
     9661 aagtcaattc gtgtctatca tgacaaatct gcttcccggc gttccgcggg tgcatctcgc
     9721 gggtgtgaga ggatctgaag tgaagatcct cgcgacatgg accggccagg tatcggtgac
     9781 tacctcatat cgctaccgga gcgggttctc cggtcgacgt cagccgtagc cgggggcttg
     9841 cttcgcgaac tcggcgatgc tgccgtccct gccgcggtgc gccggacgct cctctaccgg
     9901 tggagc
//
LOCUS       DATIFQ010000139        41001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_431585, whole genome shotgun sequence.
ACCESSION   DATIFQ010000139 DATIFQ010000000
VERSION     DATIFQ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 41001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41001
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431585"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VLE22_06390"
     CDS             <1..1275
                     /locus_tag="VLE22_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSB14068.1"
                     /translation="LRLDVESDLARVLIPPDNPFVNDTAARPEIWAIGLRNPWRFSFD
                     RATGDLWIADVGQNRAEEIDFQLASSRGGENYGWNQMEGMQCYRSGCNTAGLTLPVVE
                     YVRSEGCSVTGGFVYRGRESPGLRGTYLYGDFCTGRIWGVRREGDLWNNRLLLASGLS
                     ISTFGEDEAGEILVADYGGAIYRIGGPVDPFFTSAGVTNAASYASGLVAGSLATIFAA
                     GVKDDNGIAAADRVPLPTSVAGVSVTVNGFAAPVLAVANVNGQEQVNVQTPWQLAGAM
                     SASVVVTRDGRSSTPVDVPVLARQPGIFTVNGRDAVFVHHADNTLVTVGRPLIRGEYA
                     YFYAAGLGPVDHAPPSGSGGPRDPLAAARADVRVTLGGVPCETQYSGLAPDFVGVYQV
                     NIRVPDGVPSGTLELILQAGEARSPVVDVPVQ"
     gene            1291..2118
                     /locus_tag="VLE22_06395"
     CDS             1291..2118
                     /locus_tag="VLE22_06395"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HSB14069.1"
                     /translation="MGFDGIRVVSLESRRATEIEKLIRIQGGEPFVAPSMREVPLERN
                     EEAFRFAERLFAGEFDMMVLLTGVGTRTLDRLLATRYPAGRFAEALRSITVVARGPKP
                     AAALREMDVPVTVSVPAPNTWREILAAAAVRPERRMAVQEYGRSNEELLAGLRALGRE
                     VTPVRIYQWDLPEDTGPLREAARRIASGLASVVMFTTANQVPHLFRIASEEGVEEAMR
                     RGLGQAVVASIGPTTSEMLEEYDIQPDVVPSQSKMGILVRETAEQASGILERKRADR"
     gene            2115..2336
                     /locus_tag="VLE22_06400"
     CDS             2115..2336
                     /locus_tag="VLE22_06400"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HSB14070.1"
                     /translation="MKEKRNVWVMVGQYSSLAMLLPAATVVGYGIGYLLDNALGTSFL
                     KPVFLVLGIGAGFVQLIKQVQKDTSDREQ"
     gene            2320..2709
                     /locus_tag="VLE22_06405"
     CDS             2320..2709
                     /locus_tag="VLE22_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HSB14071.1"
                     /translation="MTANSEAAHFDRAIERIRRAMLWLSVAGAAVAAGWKGWEWGLGF
                     LAGAAVSLLNFVWLHQLVAALGEGARRPRKRLVWFLALRYVLLGVVGYVIVKFFGLNL
                     AAGLIGLLVAAAAVILEIIYELIYARA"
     gene            2696..3433
                     /gene="atpB"
                     /locus_tag="VLE22_06410"
     CDS             2696..3433
                     /gene="atpB"
                     /locus_tag="VLE22_06410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HSB14072.1"
                     /translation="MPEHELWITALFNKYLAGVANSFLSMAGVHVEDPSKPWANYIAM
                     QALVALIVIVVFAILRPRMSMDKPGRMQHCFEVVYQFLGGQADEVVGHDGKKYIHLFS
                     TIFFFILFCNLIGIIPGFESPTMFAPVPLGCALVTFAYYNTMGVQAHGAGKYLAHFAG
                     PMPIMAPLMVPIEIISHLGRLLSLTVRLYANMFAGEQVTLVFLSLVPLVVPAVFMGLH
                     VFVSFLQAFVFALLTMIYVGSAVEHEH"
     gene            3528..3818
                     /locus_tag="VLE22_06415"
     CDS             3528..3818
                     /locus_tag="VLE22_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HSB14073.1"
                     /translation="MKVKLLVLLALLVFASPMFAQTQGGTDWVSITSGFSMAIASAVC
                     GLAQAKAVAASAEGMARNPSAAPAIRFALLLGLVLIESLALYTLAIIFLKVG"
     gene            3868..4752
                     /locus_tag="VLE22_06420"
     CDS             3868..4752
                     /locus_tag="VLE22_06420"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HSB14074.1"
                     /translation="MKLNGIFPPIATPFDHNGDLYKAKVRHNIEKWNQTGLSGYVVCG
                     STGESVMLTPDEKLQLWEWVAEYTAPEKLLLAGTGMESVRETVWLTNKAAEMGYKAAM
                     VRTPHYYKNMLNRRDAQTLYFRAVADQVKIPIMIYNWPQATGVDIPPESVVELSEHPN
                     VIAIKESSGSLEKVMQMIREVKPGFQVLVGSAPTLWPSFAVGAVGAVLAFANAAPYST
                     VTIWEAHRTRETEAALDWQNRIGRAAYLVTVKYGIPGLKYAMDLNGYYGGPCRLPLSP
                     PTPEAKREIEEAFKDLKG"
     gene            complement(4762..5673)
                     /locus_tag="VLE22_06425"
     CDS             complement(4762..5673)
                     /locus_tag="VLE22_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14075.1"
                     /translation="MLRREFLAMLAATQAAKIDGSVLVHEHVLVDFVGADRIKRGRYD
                     PNEVFRAARPKLEEVKQFGCRRLLECTPDFLGRDARLMKRLADATGVEIWINTGIYGA
                     GNYRYVPPFAKQETAEKLARRWIQEAQKGVHGVRPRFIKTGVNKAPLQELDRKLVKAA
                     AITSWETGLPIASHTGDGAAALEQLEILTAQKVAPRKFIWVHAQNEKDHSAHERVARA
                     GAWVELDGIGPKSVDWHLECVRYLEVRDLLGRVLISQDAGWYHVGERGGGDYRGYTFI
                     YTDFLPKLDPEFAWRLMVENPVNAFGK"
     gene            complement(5675..6124)
                     /locus_tag="VLE22_06430"
     CDS             complement(5675..6124)
                     /locus_tag="VLE22_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14076.1"
                     /translation="MATVYRTHAVTGAQPEAIVLHCSDPRYQAHFHEFLCEHLGIRSY
                     ALIATPGGAQLLRAVDLLPKFSWAGWRWTKFVMDIAGPARFILIGHEDCRWYSEGPFW
                     HRGGNVRERVIDDMRKVREEMTERFPGVTVELYIARLDGQNAVIESV"
     gene            complement(6128..7699)
                     /locus_tag="VLE22_06435"
     CDS             complement(6128..7699)
                     /locus_tag="VLE22_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSB14077.1"
                     /translation="MFAELSQLMMPFVVLFVLLALFIAIALFSRNYLKVSPNMVAVLS
                     GRKRKLSDGRVVGYRMVRGGAALRIPLLEKVDYLSLNVFTIPLEIKRAYTGQGVPVSV
                     KAIANVKIRGDEASLHAAAERFLGMTHVQIQQVIFQTLEGHLRSILGTLTVEEINNDR
                     QSFAQKLTSEAAVDLEKMGIGVDVLTIQEISDEEEYLNALGRRRTAEVKRDAVVGEAE
                     AQRDSKIRSAVALQEGERAKFDADASIAGAQRDFQIKQAQYQAEVETEKAKAAQAGPL
                     SEARSKQAVVTEEVKIQKARTQEMISVQEQEVLRKQKELEATVIKPADAERQAAIVRA
                     EAAKMAAVLEAEGRRSALIALAEAEQEKLHKEGAGRAAALEVEGKAEAAKIEAIGLAK
                     AKAIEAQGVAEATAIRQKAEAWKQFNEAAKLQTILEKLPAILESSTGIFGAVAAPLGN
                     IDKLVVIDQGNGAAGAGTSGLGRIAQTGPSVVFNLLQQMQALGLDVPTVLKQLGVRAD
                     ASQAPGESAQSQPEA"
     gene            complement(7721..8266)
                     /locus_tag="VLE22_06440"
     CDS             complement(7721..8266)
                     /locus_tag="VLE22_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14078.1"
                     /translation="MADWFSQVSTFSVFLGIAAVGFIFLLVSLLFGEIFDHFGDGGFD
                     HDLDHGGPSFLSPRILSVFITAFGGFGAMAIHYGLSTLGASGVGFASGVFFASLIYLF
                     ARFLYGQQATTRLQASDLAGITARVVVGIPANGVGQVRCQVGEEVVDKIARSRDAQAV
                     PENTIVTVEEVLGEIVIVRPQ"
     gene            complement(8341..9402)
                     /locus_tag="VLE22_06445"
     CDS             complement(8341..9402)
                     /locus_tag="VLE22_06445"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:TIGR02089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HSB14079.1"
                     /translation="MTKYSIAVIAGDGVGPEVIPQARRVLEKTGVKYGFSLAFEEFEW
                     GAEHYFRHGKMAPPAMLDLLRPFDAILLGAVGHPDIPDHITLNGLLLPIRRGFDQFAN
                     VRPAVLYEGVESPLKGRNAGDIDMVIVRENTEGEYAQVGGFLYHHQPDEVAIQTSVFT
                     RRGIERVVRFAFDLAVKRNRKRRVTSITKSNAQGYGMVLWDRTFQSVAADYPAVETES
                     LLVDAAAMNFVRRPDTFDVVVASNLFGDILSDVSAIIAGSIGLAPSANIDPTRRYPSM
                     FEPVHGSAPDIAGKGVVNPLAAILSAGMMLDHLGQAQAASDVEKSVAAVLADGTARTP
                     DLGGSSSTSEVTEAVVAWL"
     gene            9438..10238
                     /locus_tag="VLE22_06450"
     CDS             9438..10238
                     /locus_tag="VLE22_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB14080.1"
                     /translation="MTPDFLQSAFSLKGRTALVAGASRGIGLAIARGVAGAGARTILA
                     ARSMDDLEREAASIRDAGGRAEAAHLDMTDRDSIRRFAAAYPDVDILFNVAGTNRRRR
                     FETYSREEYDWLLQTNLHGIVELTQLVGGRMVQHGKGGKVITIGSLMSVLGLPYMTVY
                     AITKSALAGMTRALAAEWARHNIQVNCIVPGFIRTDLNRHLWEIPAMVEWVKSCEANP
                     EIGKPEDVAPLAVFLASRGSDYITGQLIAVDGGFTTTATWPMEPLTTG"
     gene            10599..10826
                     /locus_tag="VLE22_06455"
     CDS             10599..10826
                     /locus_tag="VLE22_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14081.1"
                     /translation="MPLSFATDIRPLFRDSPDVDSMQGYGLDLSSYEDVKDKAAEIYE
                     RLEDGSMPCDESWPAERLALFKQWIDEGLAP"
     gene            11289..11636
                     /locus_tag="VLE22_06460"
     CDS             11289..11636
                     /locus_tag="VLE22_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB14082.1"
                     /translation="MPEDNGALRTLRTRPDLAAIRCSKGVSLDQIVDSTKISKRFLLA
                     IEDGAFDVLPGGIFNTSYLRQYARAIDYDESDLLAYYYAATGTAPQKDEQSATENRKG
                     SARSLRPPIFARF"
     gene            11572..13125
                     /locus_tag="VLE22_06465"
     CDS             11572..13125
                     /locus_tag="VLE22_06465"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB14083.1"
                     /translation="MPRRTERAQPEVSGLPFLRASSRPLRLALFLLVAAALVGAAVIE
                     GVVLETRSGRPLARVRVSLRSISGAAAVVSNRAYTDTSGRFVFSGLPAGAYFVSAEKP
                     NYASASFGQKKLNGKGSPIVLDAGARFVAELHLSRLGVLTGQIRDENGVGIENHPVLV
                     YRDGRPPKLAQRGSTDDRGVFRIPGLEPGRYHARTGPRRMEDGSGMLPTYFGHGAVLS
                     ESKAVDVRLDEETPDVDIMPRTGRLYKLTGRAAWAGISTVSLYSDLGRMTSGVGGDGR
                     FSFDQLPPGSYELLAESEGDGPRMAAYRAIFISDDIEGLILEPSTVPRLSLLCDLNDS
                     RAAVERQVSVFVRRIQPPEGQSARRFFCQEEPYIGPGTWEIGVATPPSVYVTGMSLDG
                     KPLETNRFLLAPGGNPLLTILLSAKPATLLGVVKAPGGVPSVGATVFLKPVDPDVRER
                     LFERESFRTDTNGQFRVDGLPPGTYQVVASFEYEKPDEVNWGLSQVRTVRLDEGKESS
                     VELELAGSM"
     gene            complement(13138..14544)
                     /locus_tag="VLE22_06470"
     CDS             complement(13138..14544)
                     /locus_tag="VLE22_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSB14084.1"
                     /translation="MRIGGEPATAAATREIAIPYDGTPVATVYEASKEHVEAAVAAAV
                     TAAPLMRELILDERASILRRAYQNTLDRRDDLALTVSSESGKPLREARLEVERASQTL
                     LFSAEEAHRLAGEVVPMEASPAGKGRWAMTLREPLGVIAAITPFNFPLNLPMHKIGPA
                     IAAGNAIVHKPASATPISALKMAAILEECGLPKGALNVVTGPGGTIGDLLSCHPKIAM
                     VTFTGSVEVGLRIRSLAGMKRVTLELGSNSAVILADDANLDEAVPRCVTGSFAHSGQV
                     CISVQRIFVDRKIRDVFLERFVEATRKLKIGHPHDPATEVSSLITVKDAERVEEWIAE
                     AVQSGAKLLTGGSRRHATVEPAVLADVPPHVQISCKEAFGPLVAVNSFDTLDEAIGML
                     NASDYGLQAGIYTRDLEKAFTAARKVHVGGFMINDVPQYRADHMPYGGVKLSGTGREG
                     PKYAVEEMTEIKLVCWKP"
     gene            complement(14577..15578)
                     /locus_tag="VLE22_06475"
     CDS             complement(14577..15578)
                     /locus_tag="VLE22_06475"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSB14085.1"
                     /translation="MDNVTHTLIGVILSRTGLNRLTPHAAAILMLSANAADIDVVSAL
                     GGSLRYLECHRGITHSLIAIPLMAMLPVLLVWLLARTRLPWRRAYLVSLAGAASHPLL
                     DWTNMYGVRLLSPWSGCWYRADIHSVVDAWIWAVLLLALAAPALSRLVSSEIGARPGT
                     GRGWAVLALCFFATYSFGRYLLHSRVVATLDARLYGNEAPLRVAAIPTPFNPFRWVGL
                     VETEGFYFVYDDLNLLREFDPSGGSVFLKPPASPEIERARDTETFRAFLAFSQYPLWR
                     VTPLAVPEGGKRVEVMDLRFGSPPKPRFVATAVLDHALRVERSWFAFGDLSVPGSRR"
     gene            15687..17327
                     /locus_tag="VLE22_06480"
     CDS             15687..17327
                     /locus_tag="VLE22_06480"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine exchanger family transporter"
                     /protein_id="HSB14086.1"
                     /translation="MLESLARTLAEYPILTLFVVIGLGYLLGEIDFHGFRLGVAGVLF
                     VGLAIGSLSADVALPEVVSTLGLIIFVYAVGIHSGPAFFDSFRRQGYRNSLLTVVVLL
                     FGAGIALALGYAMQLGGGRAAGLYCGALTNTPALAAARERIRDAARSEGLPEQQVRAQ
                     SDQPVVAYSIAYPIGVIGVLLSFHVARRFLRMEVPPPEEPPEIGVRDFVVKNPGVIDR
                     KVSEVLRLHKEVGFVVSRIRHDGVTDLARSETVLREGDIVVVVGDEEALERAQQIFGA
                     ASDARIELDRTILDYRRVFVSSKQVVGKRIRELDLQNKLAATITRLRRGDVDIVPDPD
                     TRLELGDWVRVLSRRDNFPAVSQFLGDSIRGTAETDFGSVAIGMVLGVMVGMAPVPLP
                     GGTTVSLGLAGGPLLVALVLGKMEHWGRVTWVMPASANLTLRQIGLLFFLAGVGVNAG
                     YNFVETLRTSGVQLLAAGAAVTFGVTLATLIVGHKLLKIPFDAMMGLMSGVQTQPACL
                     AYANSQTKSDAANLAYASVYPAATIAKIVIAQLVASWR"
     gene            complement(17332..18252)
                     /locus_tag="VLE22_06485"
     CDS             complement(17332..18252)
                     /locus_tag="VLE22_06485"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HSB14087.1"
                     /translation="MATYRNAVIIHNPSARGLFGRRASRLERAVEILRRAGHGVSAVG
                     TAGPRTASAVARNCVEKGADLILAAGGDGTINEVANGMVGSPVPMGILPGGTANVLGI
                     ELGVGVRPETAAQRLGECVPRRIATGVLRASGESAPRHFLLMAGVGFDAAIVNKVDPE
                     LKRRSGKLAFWVAGFREVFSRQPMFEVFDGSRTLQCSFALASRVRNYGGTVELARQAS
                     LASEDFAVVLIQGRRPVAYLRLLPAAFLGHLEKQRGVTALRARKLEFRAAVDAPVYVQ
                     VDGEAAGRLPASVEILPDALTLLVPPEFQG"
     gene            18296..20293
                     /gene="ligA"
                     /locus_tag="VLE22_06490"
     CDS             18296..20293
                     /gene="ligA"
                     /locus_tag="VLE22_06490"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSB14088.1"
                     /translation="MGDIGSEVEKLRRELRRHEHLYYVLDRPEITDAEYDALIRRLME
                     LEVRHPELVTPDSPTQRVGGKPREGFVTVAHSASMLSLDNALNEGELRDFDRRVASLL
                     GGEPYSYVVELKLDGLSMAVHYKDGRLSQAITRGDGAHGEDVTENARTIRSLPLKVED
                     SLARFEARGEVILNRRAFERLNQERESAGLPRFANPRNAAAGSIRVLEPTITASRRLD
                     YYAYFLLVDGAFHFDSHWESLDALERMGFKVNPNRRRCGNLEELLEFYRVWQERREEL
                     PYEIDGVVVKVDSVAQQRTLGWTAKAPRWAIAFKYPAQQQETVVEQIEVQVGRTGTLT
                     PVAHLRPVLVGGVTVSRATLHNEDEIERLGLEIGDSVLIERSGDVIPKIVRVTRKAEQ
                     PRAFVMPKECPVCGGQIVRQEGEVASRCINTNCPARLKESILHFTTRGVMDIDGMGEV
                     LVDQVVDRGLVKSLADIYDLRLEQIVELERMAEKSGKKLVENIERSRQKPLPRVLTGL
                     GIPFVGERTAAILAETFGSLDRIAAADEETLKQAEEVGPKVARSIWQFFREPHNKELV
                     ERLRKAGLQFEHAVSRKAALPLAGLTFVLTGTLPNLSRDEAKAKIEAAGGKVTGSVSK
                     KTNYVVAGEEAGSKLQKARELNVPVIDEPEMLMLLGTTAKR"
     gene            complement(20283..20657)
                     /locus_tag="VLE22_06495"
     CDS             complement(20283..20657)
                     /locus_tag="VLE22_06495"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HSB14089.1"
                     /translation="MTEAAIPLAAIVASVFLAMLAIYFRHMRYRMYHHERMAAIEKNL
                     PLPEDFFELPARPIDSAYLLRGLIWLAVGVGSVIFFVTMALTEGDKELYSVATLGLIP
                     VGVGIAYLVVRRNEMKRAETTA"
     gene            20764..21306
                     /locus_tag="VLE22_06500"
     CDS             20764..21306
                     /locus_tag="VLE22_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB14090.1"
                     /translation="MASDSTGDEIRVLLDTGRREDALRLLLERYQDRVFRLCCVLLAD
                     ESLAEDTMQEVFLRAWKGLPGYRGEAALGTWLYAIARNVCLTRLKRKPPVRTVPLDEA
                     RFQHASEPRHDGTEVRSMVMQLPERYRQVLFLYYYEDKSYDEVAAMLDLPLGTVKTQL
                     HRARKELSALLKGAYGVRAI"
     gene            21287..21733
                     /locus_tag="VLE22_06505"
     CDS             21287..21733
                     /locus_tag="VLE22_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14091.1"
                     /translation="MACERFETRIVEYVDGAPDHVLEEHLRSCAACRDAVDVLTALDQ
                     SLAANLTPPEAPPQNSRRVMRRVAELERAGRANRLPQLLDLVGIAGVAAGAVWFLTRA
                     PQLTLPGLVPSGAATVLPWVIAGACLSAGFLFALACLTQPVQPVKW"
     gene            21759..22355
                     /locus_tag="VLE22_06510"
     CDS             21759..22355
                     /locus_tag="VLE22_06510"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSB14092.1"
                     /translation="MLRALAQTLISWGPPGLLLLALLDSTGIPLPATVDALVIALAAV
                     QPGLAILAAALAVVGSSAGCMVLFYLSRKGGERYLDRYTRSGRTRKFRELFLRYGLVT
                     VFIPTLLPLPLPTKIFVISAGAFGVRWLPFLLVVLAARVPRYFGLAYLGSQLGEHSGA
                     WLRGHVWHLAAIALAMFALSVLLIRVAERSRKAAVGLG"
     gene            22366..23775
                     /locus_tag="VLE22_06515"
     CDS             22366..23775
                     /locus_tag="VLE22_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB14093.1"
                     /translation="MLSRRDFLRAATVSPLIGCRKPTASGIRIRDLGFQYEDFLYRAP
                     IKFGGLVLDRVTLLNVDCVVEDAGGRTSKGSGSMPLGNIWSFPSRKMPYEATLAAMKA
                     LAGRISKITGAYKESGHPIDINWALEPEYLKAADEVSRELALVEPIPKLCTLVTASPF
                     DAAVHDAFGKLHGRNCYRTYGPDLMQHDIGHYLGAEFQGEYLDRYVLQTAKPRMPLYH
                     LVGALDPITESDIKQRINDGLPETVPEWINYNGLTHLKIKLNGDDLKWDVGRVLAVDR
                     VAVETQKKRGVTKWFYSADFNERCKNVDYLIAFLRQVKEKTPEGFERIQYIEQPTGRD
                     LKAHRSNVMHEAAKLKPVVIDESLTDLESLQLAREMGYTGAALKACKGQSQAVLMAAA
                     AQKYKMFLCVQDLTCPGASLIHSAGLAAHIPGVAAIEANSRQYVPAANEPWVKRFPGI
                     FIVKDGTMDTSILDVPGLV"
     gene            complement(23797..24492)
                     /locus_tag="VLE22_06520"
     CDS             complement(23797..24492)
                     /locus_tag="VLE22_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB14094.1"
                     /translation="MNNDIVIRVHDLRKIYRVGEVDVHALRGVDLAVGRGEFLAIVGP
                     SGSGKSTLFHILGGLTPPTSGTVFIHGKDLLAMSESDRTELRKTEVGFVFQKYNLLPT
                     LTASDNIAIARHIAGKDFQEDPKFREVLDLLGISRRLHHKPRALSGGEQQRVAIARAI
                     VNHPSILLADEPTGNLDTENSNVVLGLLRDLNGQLGQTILMITHNPEAAAYAHRTVHM
                     RDGKVVAEPVESR"
     gene            complement(24485..25579)
                     /locus_tag="VLE22_06525"
     CDS             complement(24485..25579)
                     /locus_tag="VLE22_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSB14095.1"
                     /translation="MINKLVVENLKHRPVRTLLSVLAVGVEVTMMLTLVGLSEGMLED
                     SARRARGVGADVLVRAPGTSLIGLSSASMNEKLLGFFEQQPHVRYATGTVVYPIGGVN
                     TITGIDLEEFNRISGGFRYQEGGPFRNPDDMIVDEFYARQNKLTVGSTVRLLDRNWRV
                     CGISEPGKLARIVIPRRTLQDLTSTTGKLSSVFLKLDNPANTDKAVAALKQRLPDYPI
                     YSMEELTSLYSVNNVPGLKAFIGVIIGLSIVVGFLVVFLSMYTAVLERTREIGILKAL
                     GASPAYVLNMIFRETALLALVGSVLGILLTYGSRWVIETMVPASLIQKIVPGWWPIAA
                     VIAVLGAILGAMYPAWKAARQDAIEALAYE"
     gene            25910..26671
                     /locus_tag="VLE22_06530"
     CDS             25910..26671
                     /locus_tag="VLE22_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB14096.1"
                     /translation="MERRLGVIGGAMIRVVLYTDEPMFAEGLTHVLSSGNAFQLCEVC
                     GTIGGLSRGVAEARPEIVMLDWGPTVTLRDLLDIRSSAPWVRVVVWARSISTELAFQA
                     IEHGIHGILDKCAPAHTLLKCLEIVSEGGLWFDETLKKKILDRPAVELTRRESQLVTL
                     LCHGMSNKEIAGELRLSEGTIKVYLSRMFQKLGVKDRFELALYGLKNHVEKNGAAAFA
                     HGRDKVRRPVQSDHGPWLRSLMVVPQTEHSAANAG"
     gene            complement(26687..27694)
                     /locus_tag="VLE22_06535"
     CDS             complement(26687..27694)
                     /locus_tag="VLE22_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSB14097.1"
                     /translation="MPTRSVTPRRWLVVVSLSAAGAAAALVILPLIGSAPVDYGRAFA
                     GLSPDAEILFYARLPRVLLGLLAGGALAVAGALFQALLRDALATPYTLGVSSGASLGA
                     VLSISLGWQTAVGLPAIWLAAFGGAGLTLALVLAVATEGRRMSSFTLLLAGVTINSIC
                     LACILFLQYLADFGQSFAIVRWLLGGIDAVEYSTLGWLAGAVLPFVIYLFWKARDWNL
                     MTVGEEWAASRGVSTSRLMLAGYISGSLIAGSVTALTGPIGFIGLIVPHALRLRFGAD
                     HRFLIPCSFFVGAAFLAACDTVARTILAPTEVPVGVITAMLGGPFFIWLLRSRRRSLW
                     L"
     gene            complement(27701..28438)
                     /locus_tag="VLE22_06540"
     CDS             complement(27701..28438)
                     /locus_tag="VLE22_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB14098.1"
                     /translation="MRYNGSQVLHGASLDFEGEQLAAVVGPNGAGKSTLLGIMAGLRP
                     RYTGRCRYNGTEIGKWPRRAFARQVAFVPQSVRLEFPFTAEQVVLMGRTPHCDGLFES
                     PEDREAVERAMAITDTIRFRRRDFRSLSGGERQRVILASVLAQSPRVLLLDEPTTFLD
                     LQHQVATYNLLRDLARKGLLVVAVTHDLNLAAAYSDRVIMLRAGEIVADAAPSDVMTA
                     EMVRRVFGVDAEIQRTAAGRPWIVYGD"
     gene            complement(28468..29307)
                     /locus_tag="VLE22_06545"
     CDS             complement(28468..29307)
                     /locus_tag="VLE22_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HSB14099.1"
                     /translation="MVAGWLLALALAAPPQRIVSTAPSITEMLYALGLGDRVAGVTTF
                     CNYPPQAARKPKIGTYLRPDVEMILSLRPDVVLAEKSMIRQAITLPTLKLNVVELDDA
                     TIMEILESIRQIGALCGVPERAAKLCSSIQGGLDSVRGRTTGLPRRRVLFVVGRTPGR
                     IEDLIAAGSSSYLNEVMEIAGGENVMAGAAIAYAKVSLEEVLVRDPEVIIDMGEMSRT
                     AGVTEAEKRAVVALWNRYPNLTAVKRKRVFAVASDIFVVPGPRVIEAAREFGRLIHPE
                     VKF"
     gene            complement(29301..31544)
                     /locus_tag="VLE22_06550"
     CDS             complement(29301..31544)
                     /locus_tag="VLE22_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB14100.1"
                     /translation="MAGYFRSLILLTSMAATVAADPTQLVITGTVYDPSARPIRGARV
                     ECGSQTVVTGLDGRFNFPGGERCRAVITASGFEVREVEIAAPGDFRVDMTVAGVVERI
                     IVSATRYETTTEEAGVAATVLTRSDLERRAFPTVPDLLRETPGLQVTTTGRQGALTSV
                     FTRGGQRTGTMILIDGVPANDPGGEYNLAHLSSSDIDRIEVVRGPESALFGAEASAGV
                     VQLFTRRGDPENKIPRGSLSYERGSFQTDRWVANLLGGSGARLDYALNAEQFHTTGEF
                     SNDYYRDTTGSANVGFRFSSNTQLRTVFRTSDSVLGVPGQVGYGLVDQDAHETNRDST
                     LSLRLDDARGSSYLQRFTFGYHRVRDLYVDERLDGPYALAALVRDVRVPEPRTYLVRL
                     LDPNHLPEVPPAGTRLVSQEVTLFPFEPFLSATSRKRAGYQGTWEQPGGPMVFGYDYE
                     RQEGDVSARAVERNNHGLFLHKQQTLAGRVFLSGGLRMEHSSAFGRKVTPRGAASFLL
                     AGEHGPLSSTLFRVSAGRGITEPSLIQNFAREFFFAGNPDLRPEKTSSYEAGLVQEWF
                     SRRARTEVAVFHNSFQDLITFVSLPPPVWGSWDNVDQSRARGVEFSGRVKARNDIMFG
                     GSYMRLWSRIIHSNSPNHPFTGIGQELGRRPGHSGSVSMSIAPSRWWFQAGAILVGER
                     QDYDYNLGVTRNPGYQNVYAACSVRLNGRLSPYLRADNLLNSRYEEVLGYSSLSRGIR
                     GGLRFEW"
     regulatory      complement(31567..31763)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(31828..32205)
                     /locus_tag="VLE22_06555"
     CDS             complement(31828..32205)
                     /locus_tag="VLE22_06555"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB14101.1"
                     /translation="MDQRRGIRYELKLDCNLSSPLKSFDAMVGVTQNISRSGLLVTIS
                     APPAERSPRLGDLARVVVRLPSGQHIRGRGLNCLARVVRVSEEGGPVRIALEIRRIKF
                     DDTEDADEAVFPLSEPPEIQYIQ"
     gene            32385..34241
                     /locus_tag="VLE22_06560"
     CDS             32385..34241
                     /locus_tag="VLE22_06560"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSB14102.1"
                     /translation="MLGITKKNWKKYSILATIVFVSIVITLLLGEFRFFQHLHLKALD
                     AHFVARGREPTSGIVLLVIDQKALDHFEELQLFWHPYYAEAIRGVAEGGAKVLGMDVI
                     FAVPVTKWEPEHDRMLAEAVADTASRMPVICGYAPAVMSKQQEWPVPVNMIASALGMT
                     AFANLTADPDDFVRRQELIETPVPGSQVPPARSLALRLAEVYLGTQADFDGTRFLLGG
                     GTVPVSRDRAIAINYAGPPGAFPRISLWDFLQAARAGHKEQIRQWVEGKVVLLGPDNI
                     TDRYATPFYTAFSGPRWTTAGVEIHANTLHTLLTRRFLTATPQAGRMLALAAATGGAV
                     AIVASFSAAAAGLWLTLLLAGVIVFTHILFRFGVLLSTSELVLSCAFCVLFAVTYRFL
                     TAQTRGELFSKAVNLFIGRQLAVSLEDTQSITLTAKHAYVTILFSDIRGFTAFSESKD
                     PEVVVERLNEYLTVMVRIIMNHHGHVNKFLGDGILAVFSDDDGTNPGDHPVRAVRCGT
                     DMCLASSEFRTGVGIHTGPVVIGNVGSTDRMEYTVLGDTVNLASRLESLNKEHHSQLI
                     MSDSTKERLDGQVETAHLASVAIRGRVNKLDIFTVSALAPNQGEQAVTQKAP"
     gene            complement(34297..38742)
                     /locus_tag="VLE22_06565"
     CDS             complement(34297..38742)
                     /locus_tag="VLE22_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14103.1"
                     /translation="MRGWCRLFLAFLSIAGFGVSRAGAQGVITTAAGTDWLFPGDGRP
                     AREAPLGGNFSMAVAVDAAGDFYIADPDNQMVMRVGSDGVLTVIGGNGIPGYSGDEGA
                     ATSAALLFPAALAFSPASDLYVAAANRIRKISRGGVISTIAGLSDPGFSGDGGPATGA
                     AFNFPQDLAVDAAGVIYVADTFNHRIRKITPDGIVTTIAGVGRQGFGGDGGPATQALL
                     STPYGVSVDAANNIYVSDTFNGRVRRISPNGTITTVAGGSAPGDGVFSVMVPLSTKVD
                     SSGALYIADLLTNRVRKVVGSVITTVAGSGQSGYSGDGGPATRARLNWPSSVAIDKSL
                     NVYIADSANSRVRKVTPDGVISTVAGNGLFRTSGEGGPATAAALYLPTGTAVDPVGNL
                     YLSEPFRNRVRKISKGILTTFVGTGEEGFSGDGGPASKAALFYPSAIAFDEELGSLFI
                     ADQFNSRVRRVTPDGNIATVAGNGQAGFAGDNGPAVNATLKFPEGLAAAGGTLYIADT
                     SNQRIRMVDIHGTITTIAGTGERGFSGDNGPAVRAALSDPRGLAVDNSGNIYISDTGN
                     NRIRRIDIYGSIATIAGTGTAGFSGDGGPATRASLNGPVGLAFDNAGNLCVADEKNHR
                     IRCISSGGNITTVAGNGVQGLSGDGQPAVQAALYGPYSLAVDSAGNIFFADRFNHRLR
                     QIKATRPALLVEPVQLSFAGNSLGAAPPSQRVRLFPGVPGVTFSVTVATESGGTWLAV
                     TPKDGTMPATVEVSVKPGELPPGRYRGTVTLQAPQCTPPFVLVAVEFQVGPTLPARLA
                     ARPDALSFSLIEGSAASLKTLDVSNIGGGTLEFTVAATTTSGGGWLSAAPQSAAAPAS
                     TGASIDVRADPSGLSSGTYTGKIVLTNTQNGEQVTVPVVMSLNAVPQRMVLSPAGLAF
                     AAVQNGGAVPAQSFQVLNAGTGELTWSAKVTRLSGGVPWLSLSADSGSAAGSFSGSTV
                     EVRINPTGLAAGDYYGQIDISSPQAVNSPQSVAVALKLLPDATDPGPVVHPLGLVFRA
                     TQGVAPSSQNILVSNLSDNPISFRSARVSSGGTDWFVYLPSDATVTPERPTRIVVQPD
                     TAKLAPGIYEGSLTLLFTGGVSRTVNLLLVNAPAPAAARAGAAFAEAPCTPAKLLPVF
                     TSVGLETAVLAGWPNRLAMEAVDDCGNPMVDGLVVAEFSNNDPPLALTSQQNGKWSGI
                     WQVTSAGSPQVTIRLKASQPQKNIEGTAEFTAALRPGGDEPPVIFAGGVVSAASFAPG
                     LPVAPGAFVSIFGARLSDRVELSKVLPLEIELAGTSATIAGRRMPLLFTSEGQINAIV
                     PYDVPMNTTHQLVVKRGSRIATPETVTVAAAQPGILTRDQNGRGQGVVVHHSSGLYAD
                     AANPAKTGDILVIYCLGLGLVDPGVTAGSAAPFDPLSWVNGQYSLTIGGVEAQVLFAG
                     LAPSWAGLYQLNAIVPAGVTPGDAVPVVLRVRGQESPPVTMAIRP"
     gene            complement(38772..>41001)
                     /locus_tag="VLE22_06570"
     CDS             complement(38772..>41001)
                     /locus_tag="VLE22_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14104.1"
                     /translation="LKPEAAEEYRKLAKVWPDAAWISSRLFDLENERLAERPAQGKTY
                     ALLIGISSYRSEQVPQLRFAHRDARVFAQYLKSPRGGALPDSDVVVLTNEAATTAAIR
                     NAFETFLKVRAGPKDTVILLIAAHGVAEPERGAFLVTYDSDPQDLAATALPMADVHKL
                     LREDLSRVGRVMAYIDACRAATVGTIASRTVSVNSSVERLQEAEGEMFLFTASRAKEV
                     SYEGPEFGGGHGAFSYFLLGALNGEADRNGDNIVDLNELIEDVRAKVAEGTHDRQHPR
                     DFGSMETKAAVADVRREGIEIAKWTGLPQEPLLLAQARPPGMTRSLDLPPEGRPNLEA
                     AIAAGKVLPGEPRGAFDILGQLRKTQSPEEYLLAENKLRVDLEDRGQQVLLRYLTGDQ
                     VPQTRADFAAGTNWFGAALRLTPESLLLRSREVFCRGRTALFDKRYQEATELLERAIR
                     FDPTGAYSYNALGISYLEQAAYDRAIAAFQDAIRLAPYWAYPLHNLALTHTEAGKYDE
                     AIKTYRRAMTLAPAYSYLPYNLGLLYQRLNRRKEAEGAYRKAIALTPERAEPYNALGY
                     LKASAGRRAEAERLYRTALEKDPQLLSARHNLAVLLSERAEGLAEAIQLWRVNLLRDP
                     EYLPTLLSLGRALTRAGDTEEAIRQYQTVVRLKPDYVAARLAAADLFRKNNQPEAALD
                     QLNEALKRQPHASQVHEAIGDLEAARGREQAAKDAYSAALRFAPDGETRKRIGGKLKR
                     HR"
BASE COUNT         7596 a        12930 c        12546 g         7929 t
ORIGIN      
        1 ctccggctcg acgtcgagtc cgacttggcg cgtgtcctga ttcctccgga caacccgttt
       61 gtgaacgaca ccgccgcccg cccggagatc tgggcgattg gtctgaggaa tccgtggcgt
      121 ttctccttcg accgagccac aggcgacctc tggatcgcgg acgtcgggca gaaccgcgcc
      181 gaagagattg acttccagct ggcatccagc cggggcggcg agaactacgg ttggaatcag
      241 atggagggga tgcagtgcta ccgctcgggc tgtaacaccg cgggcctcac cctgccggtg
      301 gtcgaatatg tccgctcgga gggctgctcg gtgaccggag gattcgtcta ccgcggacgg
      361 gaatctcccg ggcttcgcgg cacgtatctc tacggcgact tctgcaccgg ccgcatctgg
      421 ggcgtgcggc gtgagggtga cctgtggaac aaccgcttgc tgctggcgag cgggttgtcc
      481 atctccacct tcggcgagga cgaggcgggg gagattctcg ttgcggatta cggcggcgcc
      541 atctaccgga tcggcggtcc cgttgacccg ttcttcacta gcgcgggagt caccaatgcc
      601 gcgagctacg ctagcgggct cgttgccggc tcactcgcta cgatctttgc cgccggcgtg
      661 aaggacgata acggaatcgc cgcggcggac cgagtaccgt tgcccacctc ggttgccggc
      721 gtgagcgtga ccgtcaacgg ctttgccgca ccggtgcttg cggtagccaa tgtgaacggc
      781 caggagcagg tgaatgtgca gactccatgg caactcgcgg gcgcgatgtc agcttctgtc
      841 gtcgtcactc gcgacggcag gtcgagtacg ccggtcgacg ttcccgttct ggcgcgccaa
      901 ccgggtatat tcaccgtcaa cggccgggat gcggtcttcg tgcatcacgc cgacaacacg
      961 ctcgttacgg ttggcaggcc gctcatccgt ggcgagtacg catactttta tgccgccggg
     1021 ctcggaccgg tcgatcatgc gccacccagc ggctcaggag gacctcgcga cccacttgcc
     1081 gcggcccgcg cggacgttcg ggtcacactc ggcggcgtac cgtgtgaaac ccagtattcg
     1141 ggtctcgccc cggacttcgt cggcgtctac caggtaaaca tccgggtgcc tgacggggtc
     1201 ccttccggta ctctggagct tatccttcag gccggtgagg cgcgcagtcc ggtggtggac
     1261 gtccccgtcc agtgaggagg tcgggagtcg atgggattcg acggcattcg agtggtcagc
     1321 ctggagagca ggcgagccac ggagatcgag aagctgatcc gcatccaggg cggcgaaccg
     1381 tttgtcgcgc cttccatgcg cgaggttccg ctcgagcgga acgaggaagc gtttcgcttc
     1441 gcggaacgcc tgtttgccgg tgaattcgac atgatggttc tgctgacagg cgtcggcacg
     1501 cgcacgctcg atcggctgct ggccacgcgc tatcccgccg gtcgcttcgc cgaggccctg
     1561 cgctcgatta ccgtggtggc gcgcggtccg aagcccgccg cggcgctgcg agagatggac
     1621 gtccccgtga ccgtctctgt gcccgcgccg aatacgtggc gcgaaatcct ggccgccgcc
     1681 gccgtgcgcc ccgagcggcg tatggcggtg caggagtatg gccgttcgaa cgaggagctt
     1741 ctcgccggat tgcgggcgct cggacgcgag gttacgcccg tacgaatcta ccaatgggac
     1801 cttcccgagg acaccggacc gctccgagag gcggcgcgca ggatcgcttc cggccttgcc
     1861 agcgtcgtca tgttcaccac cgccaaccag gtgccgcacc tttttcgaat cgcctccgag
     1921 gagggtgtcg aggaggcgat gcgccgggga cttggacaag ccgtggtcgc ttccattgga
     1981 cccacgacaa gcgagatgct ggaggagtac gatattcagc cggacgttgt gccgagccag
     2041 tccaagatgg ggattctcgt cagggagacg gcagaacagg catccggaat tctggaacgc
     2101 aagcgggcgg acagatgaaa gagaaacgga acgtttgggt tatggtagga cagtattcct
     2161 ccctcgccat gctcctgccc gccgcgaccg tcgtcgggta cggcatcgga tacctcctcg
     2221 acaacgctct tggaacctca tttctgaagc cggtcttcct ggttctcggg attggcgccg
     2281 gcttcgtcca actgatcaag caggtgcaga aggacacgag tgaccgcgaa cagtgaagcg
     2341 gcccatttcg accgggccat cgagcgcatc cgtcgggcca tgttgtggct gagtgtggcc
     2401 ggtgccgccg tggctgccgg atggaagggc tgggagtggg gcctcggatt tctcgcgggg
     2461 gccgctgtct cgctgctcaa cttcgtctgg ctccaccagt tggtggccgc gctcggagaa
     2521 ggagcccgcc gcccacgcaa gcgtctcgtg tggtttctcg cgctccgcta tgtccttctg
     2581 ggtgtggttg gctatgttat agtgaagttt ttcggtttga acctggctgc aggtctcatc
     2641 ggccttcttg ttgccgctgc cgccgtcatc ctcgaaatca tctacgagtt gatttatgcc
     2701 cgagcatgag ttgtggatta ctgcactctt caataagtac ctcgccggtg tcgccaattc
     2761 gttcctctct atggccggcg ttcacgtcga agatccgtcg aagccgtggg cgaattacat
     2821 cgccatgcag gcgcttgtag cgctgatcgt aattgtcgtc ttcgccatcc tgcgcccgcg
     2881 gatgtcaatg gacaagcccg gcaggatgca gcattgcttc gaggtggtct accagttcct
     2941 cggcgggcag gcagacgaag tcgtcggcca cgacggaaag aaatacatcc accttttcag
     3001 tacgattttt ttcttcatcc tgttctgcaa cctgatcggc atcatccccg ggtttgaatc
     3061 gccgacgatg ttcgccccgg tgccgctcgg ctgcgcgctg gtcacattcg cttactacaa
     3121 cacgatgggc gtacaggctc acggcgccgg aaagtacctg gcgcacttcg ccgggcccat
     3181 gcccatcatg gctcccctaa tggtgcccat cgagatcatc agccacctcg gccgcctcct
     3241 ctcgctgacc gttcgtcttt atgcgaacat gttcgccggc gagcaggtga cgctggtttt
     3301 ccttagtctc gttccgctgg ttgtgcccgc ggtcttcatg gggctgcacg ttttcgtatc
     3361 gttccttcag gcattcgtgt tcgcgctgct gacgatgatt tacgtcgggt cagccgtcga
     3421 gcacgagcac taattctggc tttatgccgc ttccagtgtg agcccggctg caagaggatg
     3481 acgggaacca cctcctggag cgctttatac aaaggagaaa gagaaacatg aaggtgaagt
     3541 tgctcgttct gctcgcgttg ctggtcttcg cgagcccgat gtttgcccag acccagggcg
     3601 gcaccgattg ggtgtcgatt acctcgggct tttcgatggc gattgcctcg gccgtttgcg
     3661 gcctggcgca agccaaggct gtggcagcct ccgccgaagg catggcccgc aatccctctg
     3721 ccgcacccgc catccggttc gcgctcttgc tcggtctggt gctgatcgag tctctggcgc
     3781 tctacaccct ggcgatcatc ttcttgaagg tcggctagtc ttttcagtgg aggggcgggt
     3841 tcgcgcccgc ccctttatct caaaaccatg aagttgaacg gtatctttcc ccccatcgcc
     3901 acgcccttcg accacaacgg tgatctttac aaggcgaagg tgcggcacaa catcgagaaa
     3961 tggaatcaga cgggtctctc tggctacgtg gtctgcggct ccaccggcga gagcgtcatg
     4021 ctcactccgg acgagaagct ccagctttgg gagtgggttg ctgaatacac agccccggag
     4081 aagctgctac tcgccggcac cggcatggag agcgtgcgtg aaacggtctg gctcacgaac
     4141 aaagcggccg agatgggcta caaggccgcg atggtccgca cgccgcacta ctacaagaac
     4201 atgctgaacc ggcgggacgc ccagacgctc tatttccgtg cagtcgccga ccaggtgaag
     4261 atcccgatca tgatctacaa ctggccgcag gcgactggcg tcgatattcc cccggagtcc
     4321 gtcgtcgagc tttccgagca cccgaacgtc atcgccatca aagagagctc tggaagcctc
     4381 gagaaggtca tgcagatgat ccgcgaggtg aagccgggct tccaggtgct ggtgggttca
     4441 gctccgaccc tatggccgtc cttcgcggtc ggcgcggttg gcgccgttct cgcgttcgcc
     4501 aacgccgcac cctattcaac cgtgacgatc tgggaggcgc accgcacccg cgagacggaa
     4561 gccgcgctcg actggcagaa ccggatcggc cgcgccgctt acctcgttac ggtaaagtac
     4621 ggtattcccg gcctgaagta cgcgatggac ctgaacggct actacggcgg tccctgccgt
     4681 ctgccgctct ccccacccac gcccgaagcg aaacgcgaga tcgaagaagc gttcaaggat
     4741 ctgaagggct gatcgtcctt cttacttccc gaacgcattc accggattct cgaccatcaa
     4801 ccgccaggca aactccgggt ccagcttcgg cagaaaatcg gtgtagatga acgtgtagcc
     4861 gcggtaatcg ccgccgccac gctcgccgac gtggtaccat cccgcgtcct gggagatcag
     4921 cacccgcccg aggagatcgc gcacttcgag gtaccgcaca cactcaaggt gccagtcgac
     4981 cgatttcggg ccgatcccgt cgagctccac ccatgccccc gcgcgcgcga ctctctcgtg
     5041 agccgagtgg tccttctcat tttgcgcgtg gacccagatg aatttgcgcg gtgcgacttt
     5101 ctgtgcggtc aggatctcca actgctccag ggcggcggct ccgtcgcctg tgtgagaggc
     5161 gatcggaagc ccggtctccc aggacgtgat cgcggcggcc ttcacaagct tccggtcgag
     5221 ctcctggagc ggggccttgt tcacgcctgt cttaatgaaa cgcggcctga cgccatgcac
     5281 ccctttctgc gcctcctgga tccagcgacg cgcaagcttc tccgccgtct cctgctttgc
     5341 gaagggcggc acgtacctgt aattcccggc gccgtagatt ccggtgttga tccagatctc
     5401 gacgcccgtg gcatccgcta gccgcttcat cagtctggcg tcccggccca ggaagtccgg
     5461 agtgcattcg agcagccgcc ggcacccgaa ttgcttcacc tcttcgagct tcggtcttgc
     5521 tgcacggaag acttcgttgg gatcgtagcg gccccgcttg atccggtctg ctcccacaaa
     5581 gtccaccagc acgtgctcgt ggaccagaac actgccgtcg atcttggccg cctgcgtcgc
     5641 ggccagcatc gccaggaatt cccgtcgcag catttcagac actctctatc accgcgttct
     5701 gcccgtcaag cctggcaata tacaattcca ccgtcacccc cgggaatcgt tcggtcatct
     5761 cctctcgcac cttccgcatg tcgtcgatca cgcgttcgcg aacattccca ccgcgatgcc
     5821 agaaagggcc ctcactgtac catcggcaat cctcgtggcc gatgaggatg aacctcgcgg
     5881 gtcccgcgat gtccatgacg aatttcgtcc acctccagcc cgcccaggag aacttcggca
     5941 acaggtctac tgcgcggagc agttgcgcgc cgccgggcgt ggcaatcaga gcgtacgacc
     6001 ggatgcccag atgttcgcag aggaattcgt gaaaatgcgc ctggtatcgc ggatcactgc
     6061 agtgcaggac aatcgcttca ggttgtgcgc cggtgaccgc atgcgttcgg tagacagtcg
     6121 ccacggctca ggcctccggc tgcgactgcg cgctctcacc gggcgcctgg ctggcgtcgg
     6181 ctcgtactcc taactgtttc agcaccgtcg ggacatccag accgagggcc tgcatctgct
     6241 ggagcaggtt gaacacaacc gacggtcccg tctgcgcgat ccgtccgagg ccggaagtgc
     6301 cggcgcctgc ggcgccgttc ccctggtcga tcacgacgag cttgtcgata ttgccgagtg
     6361 gcgccgctac cgcgccgaag atgcccgtgg acgactcgag gattgccggc agcttttcga
     6421 gaatcgtttg tagcttcgcg gcctcgttga attgcttcca ggcttcggcc ttctgccgga
     6481 tggccgtcgc ctcggcgacg ccctgtgcct cgatggcttt ggcctttgcg agaccgatgg
     6541 cttcgatttt ggctgcctcg gccttgccct ccacctccag tgcggcggcc cgtccggcgc
     6601 cctctttgtg aagtttctcc tgctccgcct cggcgagtgc gatcagcgcg gatcgccggc
     6661 cctcggcttc gagcactgct gccatcttgg ccgcttccgc gcggacaata gccgcctgcc
     6721 tttccgcgtc ggcgggctta attacggtcg cttccagttc cttttgctta cgcaggacct
     6781 cctgttcctg gaccgagatc atttcctgcg tgcgagcctt ctgaatcttc acctcttcgg
     6841 tgacgacggc ctgcttcgat cgcgcctcgg agagcggtcc ggcctgcgcc gccttggcct
     6901 tctcggtttc cacctcggct tggtactgcg cttgtttgat ctggaagtcc cgctgcgctc
     6961 cggcaatgga tgcgtccgcg tcgaacttcg cccgttctcc ctcctggagc gcgacggcgg
     7021 acctgatctt ggagtctcgc tgagcctccg cctcgccgac cacggcgtcg cgcttcacct
     7081 ccgccgtccg gcgccgtccc agggcgttga ggtactcctc ctcgtcgctg atctcctgga
     7141 tcgtcagcac gtccacgccg atccccatct tttcgagatc gaccgccgcc tcgctcgtca
     7201 gcttttgcgc gaagctctgc cggtcgttgt tgatctcttc caccgtcagc gtgccgagga
     7261 tcgatcgaag atggccttcc agcgtctgga aaatcacctg ttgaatctgc acgtgcgtca
     7321 ttcccagaaa gcgctccgcc gcggcgtgga gcgaggcctc atcgccgcga atcttcacgt
     7381 tcgcgatcgc cttcaccgaa accggaacac cttgccccgt gtaggctcgc ttgatctcga
     7441 gaggaatcgt aaagacgttg agcgagaggt agtccacttt ttcgagcagc ggaatccgca
     7501 gggccgcgcc cccgcgcacc atgcggtagc ccaccacgcg gccatccgat aacttccgct
     7561 ttcgtccgct gagcacggcc accatgttcg gcgagacctt caggtagttg cggctgaata
     7621 gcgcaatcgc gatgaacagc gccagcagga cgaacaggac gacaaaaggc atcattaact
     7681 gagatagttc ggcaaacata aaggcatgac tcccgaagag ctattggggg cgtacgatga
     7741 cgatctcgcc cagaacctct tccactgtga caattgtgtt ctctggaacg gcttgtgcgt
     7801 cccttgatcg ggcgatttta tccacgacct cctcgccaac ctggcagcgg acctgcccga
     7861 ctccattggc cgggatgccc acgacgaccc tcgccgttat accggccaga tcgctcgcct
     7921 gaaggcgagt ggtcgcttgc tggccgtaca gaaaccgcgc gaacaggtag atcagactgg
     7981 cgaagaaaac gccgctcgca aaacccacgc ccgacgcgcc gagcgtcgag aggccgtagt
     8041 ggatcgccat ggctccgaat ccaccgaagg ccgtgatgaa gacgctgaga atgcgcgggc
     8101 tcaggaaact cgggccacca tgatcgaggt cgtgatcgaa accgccgtcg ccgaaatggt
     8161 cgaaaatctc accgaacagc agggaaacca ggaggaagat gaacccgacg gcggcaattc
     8221 ccaaaaacac actgaaggtg ctgacctgcg aaaaccagtc cgccactcag gctcctttaa
     8281 tcagcccgag tctacataag cgcccgtccg aagtcaatgc gacccggact atcgtgcgac
     8341 ttatagccat gccaccacgg cttcggtcac ttcgctcgtg ctgctggatc cgccgaggtc
     8401 cggggtgcgt gccgttccgt ccgcgagtac ggctgcgacc gatttctcga cgtctgaagc
     8461 cgcctgcgcc tgccccagat ggtccagcat catgcccgcc gaaaggatgg ccgcgagcgg
     8521 attgacgacg cccttgcccg cgatgtccgg cgccgatccg tgtaccggtt cgaacatcga
     8581 cgggtaccgg cgtgtcgggt cgatgttggc gctcggagcg agcccgatgc tgccggcgat
     8641 gatcgccgag acatcactca gaatgtcgcc gaataggttc gacgccacca ccacgtcaaa
     8701 cgtgtccggg cgcctaacga agttcatcgc cgccgcgtcg acgagcagcg actccgtttc
     8761 gaccgccggg tagtcggccg caacactctg gaaggtccgg tcccagagca ccataccgta
     8821 gccctgcgcg ttggatttgg tgatcgaagt gacgcggcgc ttcctgttcc gcttcactgc
     8881 cagatcgaag gcgaaccgga ccacgcgctc aattccacgc cgggtaaaca cggaggtctg
     8941 gatggcgact tcatccggtt ggtggtggta gaggaatcct cccacctggg cgtattcccc
     9001 ttcggtgttc tcgcgcacga tcaccatgtc gatgtccccc gcattccgcc ccttcagcgg
     9061 agactcgaca ccctcgtaca ggaccgccgg gcgcacgttc gcgaactggt cgaatcccct
     9121 ccgaatcggc agcagcaggc cgttcagtgt gatgtgatcc ggaatgtccg ggtgtcccac
     9181 cgccccgagc agaatcgcgt cgaacgggcg caacaggtcg agcatcgccg gaggagccat
     9241 cttgccgtgg cgaaagtagt gttctgcacc ccactcgaac tcctcgaagg cgagggagaa
     9301 gccgtacttc acgccagtct tttctaacac gcgccgggct tgcgggatta cctcgggtcc
     9361 gaccccgtcg ccggcaatca ctgcgatgga gtacttggtc atagcgggtc ttccgaaagg
     9421 aatctcataa tagcaaagtg acacctgact tccttcaatc cgctttttct ctgaaaggcc
     9481 gcaccgccct tgtggccggt gcgagtcgcg gtatcggcct ggcgatcgct aggggtgttg
     9541 ccggcgccgg cgcccgtacg attcttgccg cccgttcgat ggacgatctc gagcgagaag
     9601 cggcatccat tcgggatgcc ggcggtcgcg ccgaggcggc gcacctggat atgaccgatc
     9661 gcgattccat ccggcgcttt gccgccgcgt atcccgacgt ggacatcctc ttcaatgtgg
     9721 cgggcaccaa ccggcgccgg cgttttgaga catactcgcg agaggagtac gactggcttc
     9781 tccaaacaaa tcttcacgga attgtcgaac tcacccaact ggtcggcggc cggatggtcc
     9841 agcatggcaa gggcggcaaa gtcatcacca tcggcagcct gatgtcggtt ctcggtctcc
     9901 cgtacatgac cgtgtacgcg atcaccaaga gcgcgctggc cggcatgacg cgcgccttgg
     9961 cggcggagtg ggccagacac aacatccagg tgaactgcat cgtaccgggc ttcatccgta
    10021 cggacctgaa ccggcatctc tgggagatcc cggcgatggt ggagtgggtg aaatcgtgcg
    10081 aggcaaaccc cgaaatcggc aaaccggaag acgtggcgcc cctagccgtc tttctggcga
    10141 gccgcggttc ggattacatc accggtcagt tgatcgccgt ggacggcggc ttcacgacaa
    10201 cggccacgtg gccgatggag ccccttacga cgggataggt gaagaataat ccgctcctaa
    10261 ggtggtagaa ttcccgccag agagaggggt ttgcgggttg agggctgcga gtgaactcat
    10321 gactaatcac aaacccagcc aagccgcaat gcgggccgcg gtcgccatcg ctcaagtagt
    10381 aacaggtcat tgcggtggag cgcttcagtc tccggtggtc gaatcaatcg ccaggatcat
    10441 cgaccgtgaa actggcctta gcgagttggt cgatgcaccg aagagatcat cgcagaggcg
    10501 ggcgacctga tcgaaggccg gagccaggag ctggtcatgc gggcgcgttc cgcgcttctt
    10561 tgcagcgccg acaaagcccc gagggaggac tgaaccatat gccgctgagt ttcgctacgg
    10621 acattcggcc gctgtttcgt gacagcccgg acgtcgattc gatgcaaggc tatgggcttg
    10681 acttgtcgtc ctatgaggac gtgaaggaca aggcggcgga gatctatgaa cgtctggagg
    10741 acggcagcat gccgtgcgac gaatcgtggc ctgcggagcg cctcgcgctt tttaaacagt
    10801 ggatagacga gggcttggcg ccgtaatccc agtgctttag cccacgtggg tggcgacgta
    10861 taataccgct cgggggaaaa tgcgcatcga ggagttttcg ttcggttcga tccgcattga
    10921 cggcgctacc cacgagcacg atgtggtggt ccacgtgacc tgctgagcga tttgcagccc
    10981 agcagtcgtc tcccgaaact gcacacgctt gttgtcctcg cgacccctta cagcaccgtt
    11041 cagttgatcg ccgtagacgg cggcttcacg acgacggcca tgtggcccat ggaacccctt
    11101 acgaatgtct actagagtta tgaaactatg tataccgcct aaaacatatt ttgtgcttgt
    11161 tggattattt cgactatttt gatgttggcc cttgcggcat tcttgtgcga tactgtccat
    11221 ggaaatcaag aaaccggcat acggaactcc gtttgccggt ttttacaact gaggatggat
    11281 gtagtctagt gcctgaagac aacggcgcgt tgcgaactct ccgaacgcgg ccagacttgg
    11341 ctgcgatccg gtgctcgaag ggcgtttccc tcgatcagat agtcgactcg accaagatca
    11401 gcaagcgatt tctcctggcc atcgaagacg gcgccttcga cgtgcttccc ggcggtatct
    11461 ttaatacgag ctatctccgg cagtatgccc gtgctatcga ctacgacgag tcggatctcc
    11521 tcgcctacta ttacgccgcg accggcaccg cgccccagaa ggacgagcag agtgccacgg
    11581 agaaccgaaa gggctcagcc cgaagtctcc ggcctcccat ttttgcgcgc ttctagtcgg
    11641 ccactgcggc tcgctctctt ccttcttgtt gccgctgccc ttgtcggcgc tgcagtcatc
    11701 gaaggtgtcg tcctcgagac gcgttcgggg cggcctcttg ccagagtccg agtttccctg
    11761 cgctcgatct ccggagccgc ggctgttgtc tcaaatcgcg cctacaccga cacttccggc
    11821 cgctttgtct tctctggcct tcctgcaggg gcgtatttcg tgagtgccga gaagcctaac
    11881 tacgcgtccg ccagtttcgg tcagaagaaa ctgaacggga agggctcccc aattgtcctc
    11941 gacgccggcg cccgctttgt cgccgagctt catctgagca gactcggggt tctaaccggc
    12001 cagattcgtg acgaaaacgg tgtcggaatc gagaaccacc ccgttctggt gtatcgggac
    12061 gggcgtccgc ccaagctcgc ccaacgcggc tcgaccgacg acagaggcgt cttccggatc
    12121 cccggcctcg aaccgggccg ttatcacgcc cggaccggtc cgcggcgaat ggaagacggc
    12181 tcaggcatgc tcccgaccta cttcggacac ggagctgttt tgagcgagtc gaaggccgtg
    12241 gacgtacggc tggatgaaga gactcccgac gtggacatca tgcctcgtac cggacgcctg
    12301 tacaagctta ccggcagggc ggcgtgggcc ggcatttcca ccgtaagcct ctactctgac
    12361 cttggacgca tgacatccgg agtgggaggc gacggacgtt tcagcttcga tcaactgccg
    12421 cccggcagct acgaattgct cgccgagtcc gaaggcgacg gcccgaggat ggccgcctac
    12481 cgcgcaatct tcatcagcga cgatattgag ggcctgattc tggaaccgtc cacggtgccg
    12541 cggctgtccc tgctctgtga cctcaacgac tctagggcgg cggtagagcg gcaggtttca
    12601 gtctttgtgc ggcgcattca gcctcccgaa gggcagtccg cgcggcgctt cttctgccag
    12661 gaagagccct acattggccc gggaacctgg gagattggcg tggcgacacc gcccagcgtc
    12721 tatgtaactg gcatgtcact ggatggcaaa ccgctggaga caaaccggtt cctgctggcg
    12781 cccggcggca acccactgct cacgatcctg ctttccgcga agcccgcgac gctactcgga
    12841 gtggtgaagg cgcccggcgg ggtgccgtcc gtcggcgcca ctgtcttcct gaaaccggtc
    12901 gatccggacg ttcgggaaag gctgttcgaa cgggaatcct tcaggaccga tacgaacggg
    12961 cagttccgcg tcgacggcct gccgcccggg acctaccagg tcgtagcttc tttcgaatac
    13021 gagaagcccg atgaggttaa ctggggcctc tctcaggtga ggaccgtccg gctcgatgaa
    13081 ggaaaggaat cgtctgtcga actggaactg gccggttcga tgtaggcgcc gccgcttcta
    13141 cggcttccag caaaccagct tgatttccgt catctcttcc accgcgtact tcggcccctc
    13201 gcggccggtc ccgctcagct tcacgccgcc gtacggcatg tggtcagccc ggtattgcgg
    13261 cacgtcgttg atcataaaac caccgacgtg gaccttccgc gcggcggtga aggctttctc
    13321 gagatcacga gtgtagatcc cggcctgcaa accgtagtcc gaagcgttca gcatgccgat
    13381 cgcctcgtca agcgtgtcga agctgttcac cgccaccagc ggcccgaatg cctctttgca
    13441 ggaaatctgc acgtgtggtg gcacgtccgc aagcacggcc ggctcgaccg ttgcatgacg
    13501 gcgcgagccg cccgtgagca gctttgcgcc ggactgcacc gcttcggcga tccactcttc
    13561 gacacgctcc gcgtccttga ctgtgatcag ggaactgact tcggtggccg ggtcgtgcgg
    13621 atggccgatc ttcagtttcc gcgtcgcctc aacgaaacgc tccagaaaca cgtcgcgaat
    13681 cttccggtcg acgaagatcc tctgtacgga gatgcacact tgaccggaat gagcgaagct
    13741 gcccgtgaca cagcgcggca ccgcttcgtc gagattcgcg tcgtcggcga ggatcaccgc
    13801 ggagtttgag ccgagttcga gcgtcacccg tttcatcccc gccagactgc ggatacgaag
    13861 ccccacctcg acgctcccgg tgaatgtcac catggcgatc ttcgggtggc agctcagcag
    13921 gtctccaatt gtcccgcccg gacctgtaac gacgttgagc gcgcccttgg gcagcccgca
    13981 ctcctcgaga atggcagcca tcttcagcgc gctgatcggc gtcgccgacg ccggcttgtg
    14041 gacgatggcg ttaccggcgg caatggccgg cccgattttg tgcatcggca gattcagtgg
    14101 gaaattgaaa ggagtgatgg ccgcgatcac cccgagaggt tcgcgcagag tcatcgccca
    14161 acggcccttc ccggccggcg aagcctccat cggtaccacc tccccggcca gacggtgcgc
    14221 ctcttcggcg gagaagagca gcgtctggga ggcccgctca acctccaggc gcgcctcccg
    14281 gaggggcttg ccgctctccg agctgacggt cagggccagg tcgtcacggc gatccagcgt
    14341 attctgatac gcccggcgca agatcgacgc ccgctcgtcg agaatgagtt cgcgcatcaa
    14401 cggagcggcc gtgaccgccg ccgccaccgc tgcctcaacg tgttcttttg aggcttcgta
    14461 gaccgtcgcc accggggttc cgtcgtacgg gatggcgatt tcccgtgttg cagccgccgt
    14521 ggccggttca ccgccgattc gcatcgggta ttccatacag ccatgatagt gcggcctcag
    14581 cggcgtgaac ccggcacgga gagatcgccg aaggcgaacc acgaacgctc gacccgtagt
    14641 gcgtgatcca gaacggcagt tgcgacgaag cgcggcttcg gcggcgaacc gaaacggaga
    14701 tccattacct caacacgctt tcctccctcc ggtacggcaa gcggcgtcac gcgccataac
    14761 gggtactgag agaaggcgag aaaggcgcga aatgtctccg tgtcgcgggc gcgctcgatc
    14821 tccggacttg ccgggggctt gaggaagaca cttccccctg acgggtcgaa ctcacgcagc
    14881 aggttcagat cgtcatagac aaaatagaag ccctcagtct cgacgaggcc gacccaacgg
    14941 aacgggttga atggcgtcgg tatcgccgcc acccgaagcg gcgcttcgtt gccgtagagc
    15001 cgcgcgtcaa gcgtcgccac tacacgggaa tggagcaggt accgtccgaa actgtacgtc
    15061 gcgaaaaagc acagcgcgag gacggcccag ccgcgccccg tgccgggacg tgcaccgatc
    15121 tccgagctga ccagccgtga cagcgctggc gcggcgaggg ccagcaatag tacggcccag
    15181 atccatgcat cgacgaccga gtggatatcc gcgcggtacc agcacccgga ccacggtgaa
    15241 agcagtcgta ccccgtacat gtttgtccag tcgagcagcg gatgcgaagc cgcacccgcc
    15301 agagagacaa gataggcccg tctccacggt agccgtgtgc gcgcgaggag ccaaaccagc
    15361 agcacgggca gcatcgccat caacggtatg gctatcaggg aatgtgtaat cccgcggtgg
    15421 cactcgagat atcgcagaga cccgcccagg gcgctgacaa cgtcgatgtc cgccgcgttg
    15481 gcggacagca tcaggatcgc agccgcgtgc ggcgtgagcc ggttcaatcc ggtgcggctc
    15541 agtatgacgc cgatcagcgt gtgcgtgacg ttgtccatgc agcgggaact acctgcgtaa
    15601 catgcccttg acaggagcgt caagcgcagg gaatcactgt gttggaacta tgatgatatg
    15661 ggtggttctt acacttcagg ctctctatgc tcgaatcgtt ggcgcgcaca ctggccgagt
    15721 acccgattct gacgctcttc gtggtcatcg gccttgggta ccttctcggg gagatcgact
    15781 ttcacggttt ccggctgggc gtggcggggg tgctgtttgt ggggctcgcc atcgggtcgt
    15841 tgagcgccga cgtggcgcta cccgaagttg tgtcgacgct gggcctgatc atcttcgtgt
    15901 atgccgttgg gattcactcc ggcccggcat ttttcgattc gttccgccgc cagggatacc
    15961 gtaacagcct gctcacggtt gtggtgctgt tgttcggtgc ggggattgcg cttgccctcg
    16021 gatatgcgat gcagctcggc ggcggccgtg cagcgggcct gtactgtgga gcgctgacca
    16081 acaccccggc gctggccgcg gcccgcgagc ggattcgaga tgccgcccga tcggagggcc
    16141 tgccggaaca gcaggtgcgt gcgcagtcag atcaaccagt ggtggcctac agcatcgcct
    16201 atccgatcgg tgtgatcggc gtgctcctgt cgttccatgt tgcccgcagg tttcttcgga
    16261 tggaagtgcc gcctccagag gagccgcccg agatcggcgt aagggacttc gttgtcaaga
    16321 accccggagt gatcgaccgc aaggtctccg aggttctccg tctgcataag gaggttggct
    16381 ttgtcgtcag tcgcatccgg cacgacggcg taaccgacct cgccagatcg gaaacagttc
    16441 tgcgtgaagg agacatcgtc gttgtggtcg gcgacgagga agccctcgaa cgggcacaac
    16501 agattttcgg ggcggctagt gatgcgcgaa tcgaactgga ccggacgatt ctcgactacc
    16561 gaagggtatt tgtctcaagc aagcaagtgg tcggcaagcg catccgtgaa ctggatctcc
    16621 agaacaagct cgcggcgacg atcacgcgat tgcggcgcgg agacgtcgat atcgtcccag
    16681 acccagatac ccgcctggaa cttggcgact gggttcgggt actctcccgc cgggataact
    16741 ttcccgctgt ctctcagttc cttggcgact ccatccgcgg caccgctgag accgatttcg
    16801 gctcggtagc gattgggatg gttctgggcg tgatggtcgg catggcaccg gtcccgcttc
    16861 caggaggcac aacggtgagc ctcggtcttg ccggtggccc gcttctggtc gcgctggtgc
    16921 tagggaagat ggagcattgg gggcgggtca cctgggtaat gccggcgtcg gccaacctca
    16981 cgctgcggca gatcgggctt ctgtttttcc tggccggagt gggcgtaaat gcaggctaca
    17041 acttcgtcga aacacttcgt acgagtggtg tgcaactgct cgccgcaggc gcggcggtga
    17101 cattcggtgt gacgctggct accctgatcg tcggccacaa gctactcaag atcccgtttg
    17161 acgccatgat ggggcttatg tccggtgtgc agacccagcc cgcctgcctg gcgtatgcca
    17221 acagccagac gaagtccgac gcggccaatc tcgcgtacgc cagcgtctac ccagcggcga
    17281 ccatcgctaa gattgtgatc gcccaactcg tcgcgtcgtg gcggtgatcg ctcaaccctg
    17341 gaattcgggg ggaacgagca aggtcaacgc gtcaggcaag atctccactg acgccggcag
    17401 ccgtccggcc gcttctccat ccacctggac gtaaacggga gcatcgacgg cagcccggaa
    17461 ttcgagtttc ctcgcccgca gagcggtaac gccgcgctgc ttctccaggt ggccaaggaa
    17521 agccgcaggt aggagccgca ggtacgccac cggccggcgg ccctggataa ggacgaccgc
    17581 aaaatcttcg ctcgcgagtg atgcttggcg ggcgagttcg actgtgccgc cgtaatttcg
    17641 cacacggctc gccagcgcga atgagcactg gagagtgcgg ctgccatcga aaacctcgaa
    17701 catcggctgc cggctgaaca cttcgcgaaa ccctgccacc cagaaagcca gcttgccgct
    17761 ccgccgcttt agctccgggt ctactttgtt tacgatcgcg gcatcgaagc cgacgccagc
    17821 catgagcaga aaatggcggg gcgccgattc gccgctggcc cgcaggacgc ccgtcgcgat
    17881 cctccgcgga acacactctc cgagtcgctg agccgccgtc tccggccgga cgccgactcc
    17941 gagttcgatg ccgagcacgt tggcggtgcc accgggaagg atgcccatcg gtactggtga
    18001 gccaaccatc ccgttcgcga cttcgttgat cgtgccatcg ccgccggccg ccagaatcag
    18061 gtcggcgccc ttctcaacgc aattccgcgc gacagcgctg gccgttctcg gacctgccgt
    18121 tccgacggcg gataccccgt gccccgccct tcgcaggatc tcgaccgccc gttcgagcct
    18181 cgacgcacgc ctcccgaata ggcctcgtgc ggaagggtta tggatgataa cggcgttacg
    18241 gtaggttgcg atgagtcagg tttcctttgg agacccacaa ttataataaa cgcggatggg
    18301 cgacattggc tcggaagtgg aaaagttgcg ccgggagctt cgccggcacg agcacttgta
    18361 ctacgttctc gatcggccgg agatcaccga cgcggaatac gacgctctca ttcgccgtct
    18421 catggagctg gaagtgcggc atccggagct tgtgactccg gactcgccca cgcaacgtgt
    18481 cggcggtaag cctcgcgaag gcttcgtcac ggtggcgcac agcgcgtcaa tgctcagcct
    18541 cgacaacgcc ctcaacgagg gggaactgcg cgatttcgac aggcgagttg cttcgctcct
    18601 tggcggcgag ccgtacagct atgtcgttga gttgaagctc gacggattgt ccatggcggt
    18661 ccattacaag gatggccggc tcagccaggc cattactcgt ggggacggcg cgcatggcga
    18721 agacgtaacc gagaatgcgc ggaccatccg ctcgctcccg ctgaaagtcg aggacagtct
    18781 tgcgcgattc gaggcgcgtg gcgaggtgat cctcaaccgg cgcgccttcg aacggctcaa
    18841 ccaggaacgc gagagtgccg gattgccgcg gttcgccaac ccgcgtaatg ccgccgccgg
    18901 atcgatccgt gtgctcgaac cgaccatcac cgcctcccgg cggctcgact actacgccta
    18961 tttcctcctc gttgacggag ccttccattt cgacagccac tgggaatcgc tcgacgccct
    19021 ggagcgaatg ggcttcaagg tgaacccgaa ccgtcgccgc tgcgggaatc tggaggaatt
    19081 gctcgagttc tatcgggtgt ggcaagagcg gcgggaggaa ctgccttacg agatcgacgg
    19141 cgtcgtcgta aaagtcgatt ccgtggccca acagcgcacc ctcgggtgga cggctaaggc
    19201 gccgcgatgg gcgatcgcgt tcaagtatcc ggcgcagcag caggaaacgg tcgtcgagca
    19261 gattgaggtg caggtcggac ggactgggac gctgactccg gtagcgcacc tgcgcccggt
    19321 gctggtgggc ggcgttaccg tatcccgcgc tacgctgcac aacgaggacg agatcgagcg
    19381 cctcggcctg gagatcggcg actccgtgtt gatcgagcgc agcggcgacg tgatcccgaa
    19441 gatcgtgcgg gtcacccgaa aagccgaaca accacgcgcg tttgtgatgc cgaaagagtg
    19501 tccggtctgt ggcggacaga tcgtgcgcca ggagggcgag gtcgccagcc gctgtatcaa
    19561 tacgaactgc ccggcgcgat tgaaggaatc gatcctccac ttcaccaccc gaggagtcat
    19621 ggacatcgac ggcatgggcg aggtgctggt cgatcaggtc gtggatcgtg gactggtcaa
    19681 gagtctcgcc gatatctatg atctccggct ggagcagatc gtcgaactgg agcggatggc
    19741 cgagaaatcc ggaaagaagc tcgtcgaaaa cattgagcgt tcgcgacaga aaccgcttcc
    19801 gcgggtgtta accggactcg gcatcccctt cgtcggggag cgcaccgccg cgatcctggc
    19861 ggagacgttc ggtagcctgg accggatcgc agcggcggat gaggagacgc tcaagcaggc
    19921 cgaagaggtt gggccgaagg tcgcccgcag catctggcag ttcttccgcg agccgcacaa
    19981 taaggaactg gtggagcgac tccgcaaggc cgggctgcaa ttcgagcatg ccgtctcccg
    20041 gaaggcggcg ctccccctgg ccggactcac gttcgtgctg accggaacgc taccgaatct
    20101 gagccgcgac gaggcgaagg cgaagatcga agccgctggc gggaaagtga ccggctcggt
    20161 cagcaagaag actaactacg tcgtagccgg cgaggaagcc ggatcgaagc tccagaaggc
    20221 ccgcgaactg aacgtccccg tcatcgatga accagaaatg ctcatgctgc tggggactac
    20281 ggctaagcgg tagtctcagc cctcttcatc tcattccgcc gaacgacgag ataggcgatg
    20341 ccgacgccga ctggaatgag cccgagggtc gctacggaat acagttcctt gtccccctcc
    20401 gttagcgcca tcgtcacgaa gaagatgaca ctgccgaccc ccaccgccag ccagatcagg
    20461 ccccgcaaca gataagcgct atcgatgggg cgtgccggaa gttcgaaaaa gtcctcgggc
    20521 agcgggaggt tcttctcgat agcggccatg cgttcgtgat ggtacatgcg gtaacgcatg
    20581 tggcggaaat agatggccag catggctagg aacacgctgg ctacgatggc tgccagtgga
    20641 attgcagctt cggtcattgg catggtcttc aggtctctcc gccctttaga cctcattgtt
    20701 cgccggcttt gtttcagatg ggtactgaaa ctttcactcg cctgtgtgta gtctaagagg
    20761 caggtggcga gcgactccac cggagacgaa attcgtgtcc tcctcgacac cggacgccgg
    20821 gaggatgccc ttcgtctgct tctggagcgt tatcaggatc gcgtctttcg gctgtgctgt
    20881 gttcttttgg ccgatgagtc cctcgcagag gacaccatgc aagaggtttt cctccgcgcc
    20941 tggaaagggt tgcccggcta ccgcggcgag gcggcgctcg gcacctggct gtacgcgatc
    21001 gctcgaaatg tctgtctcac ccgcttgaag cggaaaccgc ccgttcggac tgttccgttg
    21061 gacgaggcgc gatttcagca cgcaagcgaa ccgaggcatg acggcacgga agtccggtcc
    21121 atggtgatgc agcttccgga gaggtaccgc caggtattgt tcctctacta ttacgaggac
    21181 aagtcctacg acgaggtggc ggcgatgttg gacctgcctc tgggtaccgt gaagacgcag
    21241 ctccatcgtg cgaggaagga attgagtgcg ttgttaaagg gggcctatgg cgtgcgagcg
    21301 atttgaaacg cgaatcgtgg agtacgtgga cggcgcgccc gaccatgtgc tggaggagca
    21361 tctccgctcc tgcgcggcgt gccgggacgc ggtggacgta ctcaccgccc tggaccagtc
    21421 gctggccgcg aatctgacac cgccggaggc gcctccccag aacagccgga gagtgatgcg
    21481 aagggtggcg gaactggagc gcgccgggcg cgcaaatcgt ctccctcaac tgctggacct
    21541 tgtcggaatc gccggtgtgg ctgcgggtgc tgtctggttt ctgacccggg ctccgcagtt
    21601 gaccctcccc ggcctcgtgc cctccggcgc ggccacagtc ctgccgtggg tgatcgcggg
    21661 ggcgtgcctg tcggcggggt ttctgtttgc gctcgcctgc ctgacgcaac cagtccagcc
    21721 ggtaaaatgg tagtctgtcc cttcgacagt cggaggattt gctgcgcgcg cttgctcaaa
    21781 cactgatctc gtggggtccg cccgggctcc tcttactggc gctgctggac tcgactggta
    21841 tcccgcttcc agcgacggtc gacgcactcg ttatcgcgct ggcggcggtt cagcctgggc
    21901 ttgcgattct ggccgcagcc cttgcagtcg tcggttcgtc ggccggctgc atggtgttgt
    21961 tctatctttc gcggaagggt ggggagcgct acctcgatcg gtacacccga tccggacgca
    22021 cccggaagtt ccgggaactg tttttgcgat acggcctggt gacggtgttc atccccaccc
    22081 ttctgcctct gccgcttccg accaagatct tcgtgatctc agccggcgcc ttcggagtcc
    22141 ggtggctgcc gtttctcctc gtcgtgctgg ccgcaagggt cccccgctac tttggtcttg
    22201 catatctcgg ttcgcaactc ggtgagcatt ccggcgcctg gctgcgcggc cacgtctggc
    22261 acctcgcggc gattgccctc gcgatgtttg cgctgtcggt ccttctgatc agagtcgcag
    22321 aacgctcacg caaggcggcc gtcggattgg gatagaatcg cggacatgtt atcccgtcgc
    22381 gattttctcc gcgctgcgac cgtttctccg ctgatcggat gccgtaagcc tacggcatcc
    22441 ggcatccgca ttcgggacct cggctttcag tacgaggatt tcctctatcg cgcgccgatc
    22501 aaattcggcg gcctcgtcct cgaccgtgtg acgctgctga atgtcgattg tgttgtcgaa
    22561 gatgccggcg gacgaacgtc gaagggctcc gggtcgatgc ctctcggcaa catttggtcg
    22621 tttccgtctc ggaaaatgcc gtacgaggcg acgctcgcag ccatgaaggc actcgccggg
    22681 cggatctcga agatcaccgg tgcatataag gagtccggac accccatcga tatcaactgg
    22741 gctctggagc cggaatatct gaaagctgcg gacgaagtct cgcgggaact ggcgctcgtc
    22801 gaacccatcc cgaagctgtg cacgcttgtg accgccagcc cgttcgatgc cgcggtccac
    22861 gatgcgttcg gcaagcttca cggccgcaac tgctaccgga cctacggtcc cgacctgatg
    22921 cagcacgaca tcggacatta tctaggcgcg gaatttcagg gagagtacct cgaccggtat
    22981 gtactccaga cggccaagcc ccggatgccg ctgtatcacc tcgtcggtgc gctcgatccg
    23041 attaccgaat ctgatatcaa gcaacggatc aacgacggac tcccggagac cgtgcccgag
    23101 tggattaact acaacggact gacgcacctc aagatcaagc taaacggtga cgacctgaag
    23161 tgggatgtag gccgcgtgct ggccgttgat cgggtggcgg tcgagacgca gaagaagcgc
    23221 ggcgtcacga aatggttcta ctcggcggac ttcaacgagc gctgcaagaa tgtggactac
    23281 ttgatcgcgt ttctgaggca agtgaaggag aagacgcccg aaggcttcga gcggattcaa
    23341 tacatcgagc agcccaccgg ccgcgatctc aaagctcacc ggtcgaacgt gatgcacgag
    23401 gcggcaaagc ttaagcccgt agtgatcgac gagtcgctaa ccgatcttga aagcctacaa
    23461 ctggcgcgcg agatgggata caccggagcg gcgctcaagg cgtgtaaggg acagagccag
    23521 gccgtgctga tggcggcggc tgcacagaag tacaaaatgt tcctctgcgt gcaggatttg
    23581 acctgtccgg gtgcgtcgct cattcactcg gccgggcttg ctgctcacat ccctggcgtt
    23641 gccgcgatcg aggcgaattc gcgccagtac gtcccggctg ccaacgagcc ttgggtgaag
    23701 cgatttcccg gcatcttcat cgtcaaagac gggactatgg acaccagtat tcttgacgtc
    23761 cccggtctcg tgtagggacg cgccgccggc atattcctaa cgtgattcca ccggttctgc
    23821 gaccacttta ccgtcgcgca tgtggacggt tcggtgggcg taggccgccg cctcggggtt
    23881 gtgcgtgatc atcaggatgg tctggccgag ctgtccgttg agatcgcgaa gcagccctag
    23941 cacgacgttc gaattctccg tgtcgaggtt gcctgtgggt tcgtccgcca acagaatcga
    24001 aggatgattc acgatcgctc gcgcgatcgc cacgcgttgt tgctctccac ccgacagcgc
    24061 ccgtggcttg tgatgcaggc gccggctgat gccgagcaga tccaaaactt cccggaactt
    24121 tggatcttcc tgaaagtctt tgcccgcaat gtgacgcgcg atggcgatgt tgtccgacgc
    24181 agtcagcgtc ggcagtaggt tgtacttctg gaaaacgaaa cccacctcag tttttcgaag
    24241 ttccgtacga tccgactccg acatcgcgag gaggtctttt ccatggataa agaccgtgcc
    24301 ggaggtgggc ggagtaagcc cgcccagaat gtggaagagc gtcgactttc cgctcccgga
    24361 cgggcccacg atggcgagaa actctccgcg gccgaccgcg aggtccactc cgcgcagcgc
    24421 gtgaacgtcc acctctccca cgcggtagat cttgcgaagg tcgtgcacac gaatgacgat
    24481 gtcgttattc atacgccagc gcctcgatcg cgtcctgtcg cgcggccttc cacgccggat
    24541 acatggctcc tagaatcgcc ccgaggacgg cgatcactgc ggcgataggc caccacccgg
    24601 gcacgatctt ctggattagc gacgccggca ccatcgtctc gatcacccaa cgcgagccgt
    24661 acgtgagcag gatgcccaaa accgacccga cgagtgcaag cagcgcggtc tcgcggaaaa
    24721 tcatgttaag aacatacgcg ggcgaggctc cgagcgcctt caggatgccg atctctctgg
    24781 tccgttcgag caccgccgtg tacatcgaga ggaacactac caggaaaccg acaacgatcg
    24841 aaagcccgat tatcaccccg atgaacgcct tcagacccgg cacgttgttc accgaataga
    24901 gcgaggtaag ttcctccatc gagtagatcg gataatccgg caggcgctgc ttgagcgccg
    24961 ccaccgcctt gtccgtgttc gctggattgt caagcttcag gaaaaccgag ctcagcttgc
    25021 cagtcgtact cgtgaggtcc tgaagcgtcc ggcgggggat cacaatgcgc gcgagcttgc
    25081 ccggctcgga gatgccgcac acgcgccagt tccggtccag cagtcgcacg gtggacccca
    25141 ccgtcagttt gttctgccgg gcatagaact cgtccacgat catgtcgtca ggatttcgaa
    25201 acggcccacc ctcctgatac cggaatccac cgctgattcg gttgaactcc tccagatcga
    25261 tgcccgtgat cgtgttcact ccaccgatcg gatagacgac cgtgccggtg gcgtagcgga
    25321 catgcggttg ctgctcgaag aagccgagca gtttttcgtt catcgacgcc gagctcaagc
    25381 cgatcagcga cgttcccggc gcacgcacca acacatcggc gccgacgcct cgcgcccgcc
    25441 gggccgagtc ctccaacatt ccctcgctca agccgaccag cgtcagcatc attgtgacct
    25501 cgacgccgac cgcaagcacg ctgagcaacg tccgcaccgg ccggtgcttc aggttttcaa
    25561 cgacaagttt attgatcaag aaaggagttc cttcgaactc taggagctcc gcgcgggacg
    25621 cgcgggacca tgttccattg tagcgatgag acaaagcttc ccgcgcgttc cacatcgtct
    25681 tcacggactg cgccatcgct cgtaccagta cgagcaagag cgaagtaata gaacgctaag
    25741 taattgagta catgtgagaa atgtaccggt acggttctgt gcgtggcact ccagtttggg
    25801 tcttttcacc tgcaacgtat cagcgtattc tgaacttact ccgcgctatt cttcagccgg
    25861 ggaagcaaat acctctggca aaacgacgac acaacgcggg gtgccgccaa tggaacggcg
    25921 gctgggtgtg attggaggag cgatgatccg tgtggttctg tacaccgacg aaccgatgtt
    25981 cgcggagggc ttgacgcatg tgctgtcatc cggaaatgcg tttcagctct gcgaggtctg
    26041 tgggaccatc ggcggtctgt ctcgcggcgt cgcggaggcg cgccccgaga tcgtcatgct
    26101 cgattggggt cccaccgtta cgcttcggga tctgctggac attcgctcaa gcgcgccttg
    26161 ggtgcgcgtc gtggtatggg cccgttcgat ctcgaccgag ctcgcttttc aggccattga
    26221 acacggcatt cacggaatcc tggacaagtg cgcacccgct cacactctct tgaagtgcct
    26281 ggagatcgtc tccgagggcg ggctctggtt cgatgagacc ctaaaaaaga aaatcctcga
    26341 ccgtcctgct gtggaactca cccggcgcga aagccagttg gtcacgctgc tgtgtcacgg
    26401 gatgagcaac aaggaaatag ccggcgaact caggctgtcc gaaggcacca tcaaagtgta
    26461 tctatcccgg atgttccaga aactcggcgt gaaggaccga tttgagctgg cgttgtacgg
    26521 cctgaaaaac cacgtcgaga agaacggagc cgccgccttt gcgcacggac gggataaagt
    26581 ccggcggccc gtccagagcg atcacgggcc atggctccgg tcgctcatgg tcgtacccca
    26641 gacggagcac agcgcggcca acgcgggctg agcggtttcg tccgcttcac agccagaggc
    26701 tgcggcgccg tgagcgcaac agccagatga agaacggtcc gcctagcatc gccgtgatca
    26761 cgcctaccgg cacttccgtg ggtgcaagga tcgttcgcgc caccgtgtca caagccgcca
    26821 gaaacgcagc gcccacaaag aacgagcacg gaatcaggaa ccggtgatcg gccccgaagc
    26881 ggagccgcag cgcgtgcggc acaatcagtc cgataaaccc gatcggcccc gtcagtgccg
    26941 tgacggaccc cgcgatcagc gatcccgaaa tgtagcctgc cagcatcagg cgcgaggtgg
    27001 agacaccgcg cgacgcggcc cactcctctc ccaccgtcat caggttccag tcgcgagcct
    27061 tccagaacag gtagatgacg aatggcagta cagcgcctgc cagccacccc agcgtcgaat
    27121 actccactgc gtcgatgccc cccaacagcc accggacaat cgcgaacgac tgcccgaaat
    27181 ccgcaaggta ctggagaaac aggatgcacg ccaggcagat gctgttaatc gtgacgcccg
    27241 ccaacagcaa agtaaacgac gacatacgac gaccttccgt ggcgactgcc agcaccagcg
    27301 ctagcgtcag tcccgctcct ccaaaggccg caagccagat ggccggaaga cccaccgccg
    27361 tctgccagcc gagcgaaatc gacagtacgg ctcccagcga tgcgccgctc gacacgccca
    27421 gcgtgtatgg ggtggccagg gcatcgcgca gcagcgcctg aaacaacgct cccgccaccg
    27481 ccagtgcgcc tcccgccagc agtccgagaa gaacccgagg caggcgggca taaaacagga
    27541 tttccgcgtc cggagagagt ccggcaaacg cccgcccgta gtcgactgga gccgagccga
    27601 tcagcggaag aatcaccaac gcagcggccg ccccggcggc gctcagactc acgacaacca
    27661 gccaccgccg cggcgtcacg cttcgagtcg gcatctctcc tcagtctccg tacacgatcc
    27721 acggacgtcc cgccgccgtc cgctggatct cggcgtccac gccgaatacc cggcgcacca
    27781 tctcggccgt catcacgtcc gacggtgcgg cgtccgccac aatttctccg gcgcgcagca
    27841 tgatgacacg gtccgaatag gccgcggcga gattcaaatc gtgggtcacc gccaccacaa
    27901 gcaatccctt ccgtgcgaga tcgcgcagca ggttataggt cgctacctgg tgctggaggt
    27961 cgaggaacgt cgtaggctcg tcgagaagca gtacccgggg cgattgcgcc agcaccgacg
    28021 ccaggattac cctctgccgc tcgccgccgc tcagcgagcg gaaatcgcgc cgacggaagc
    28081 gaatcgtgtc ggtaatcgcc atggcgcgct ccaccgcctc gcggtcttcc ggggattcga
    28141 acaacccgtc acaatgcggc gtacggccca tcagcaccac ctgctcggct gtgaacggga
    28201 attcgagacg caccgactga ggcacgaatg cgacttgccg cgcgaatgcc cgccgtggcc
    28261 atttcccgat ctcggtgccg ttgtagcggc accgtccggt gtagcggggc cgcaagccgg
    28321 ccatgatccc gagcagcgtg gatttcccgg cgccgttagg cccgaccacg gccgcgagtt
    28381 gttccccctc gaagtcgagc gaagccccgt gcagcacctg gctgccgttg taacgcatgc
    28441 cgacttcgca caattggaac aggccgctca gaacttcacc tccggatgga tcagcctccc
    28501 gaattcccgt gccgcctcaa tcacccgcgg gcccggaacc acaaagatgt ccgacgccac
    28561 agcgaagacc ctctttcgtt tgacggcggt gagattcgga tagcggttcc acagcgcgac
    28621 cacggccctc ttctcggctt ccgtcacgcc cgctgttcgt gacatctcgc ccatgtcgat
    28681 gatcacctcc ggatcccgca cgagtacctc ttcgagcgag accttcgcgt aagcgatcgc
    28741 ggcaccggcc atcacgttct ctcctcccgc gatttccatg acctcgttca ggtaggatga
    28801 actccccgcg gcgatcaggt cttcgatcct ccccggcgtc cgcccgacga cgaacagcac
    28861 gcggcggcgg ggcagccccg ttgtcctccc ccggaccgag tccaggccac cttgaatcga
    28921 gctgcacaac tttgccgcgc gctccggcac tccgcacaag gcgccgatct ggcggatcga
    28981 ctcgagaatt tccatgatgg tcgcgtcgtc cagttccacc acgttgagtt tgagcgtcgg
    29041 cagagtgatc gcttggcgga tcatgctttt ctcggccaac actacgtccg gcctcaacga
    29101 gaggatcatt tccacatcgg ggcgcaggta cgtcccgatc tttggtttcc tcgccgcttg
    29161 cggcggatag ttgcagaacg ttgtcacgcc cgccacgcgg tcaccgagtc ccagcgcgta
    29221 aagcatctcg gtgatgcttg gcgccgtcga cacgatccgc tgcggcggcg ccgccagtgc
    29281 gagtgcgaga agccagccgg ctaccattcg aacctgagcc ccccacggat accgcgcgaa
    29341 aggctgctgt agccgagcac ttcttcgtac cggctgttca ggaggttatc ggcgcgcagg
    29401 tatggcgaca ggcggccgtt gagccgcacg gaacaggccg cgtatacatt ctgatagccc
    29461 ggattccgtg tgacgccgag gttgtagtcg tagtcctgcc gctcgcccac gagtatcgcc
    29521 ccggcctgga accaccagcg gctcggtgcg attgacatag aaaccgaacc cgagtgcccc
    29581 ggacgccggc ccaattcctg cccgattcct gtaaacgggt ggttcggcga gttcgagtgg
    29641 atgatccggc tccacaggcg catgtacgag ccgccgaaca tgatgtcgtt cctcgccttc
    29701 acccggcccg aaaactcgac tccgcgtgca cggctctggt ccacgttatc ccagcttccc
    29761 cacaccggtg ggggaaggga gacaaaagta atcaaatcct ggaacgaatt gtggaacacc
    29821 gccacttccg tgcgcgctcg cctgctgaac cactcctgaa cgagacccgc ttcgtagctc
    29881 gatgtcttct ccggccgaag gtctggattt cccgcgaaga agaactcgcg cgcgaaattc
    29941 tggatcaggc tcggttcggt gatgccccgg ccggcgctca cccggaacag cgtggacgag
    30001 agaggtccgt gttcccctgc aagaaggaag ctggctgccc cacgcggcgt gacctttcgg
    30061 ccgaacgcac tcgagtgctc catccgcaaa ccaccggaaa ggaacaccct tcccgccaga
    30121 gtctgctgtt tgtgcaggaa cagcccatgg ttgttcctct cgaccgcccg ggccgaaacg
    30181 tcgccctcct gccgttcgta gtcgtagccg aacaccatcg gcccgccggg ttgttcccaa
    30241 gttccctggt agccggcccg cttgcgggag gtggcgctca ggaacggctc aaacgggaac
    30301 agcgttacct cttggcttac cagccgggtt ccagcgggcg ggacttccgg caggtggttc
    30361 gggtcaagca atcgcacgag gtacgtccgc ggctcgggaa cgcgcacgtc acgcaccaat
    30421 gctgccagcg cgtaagggcc atcgagtctc tcgtcgacgt aaaggtcgcg tacgcggtga
    30481 tatccgaatg tgaaacgctg gaggtaactg ctgccacggg cgtcgtccag acggagcgag
    30541 agcgtggagt cgcggtttgt ctcgtgagcg tcctggtcca ctagcccgta accgacctgc
    30601 cccggtacgc cgagcaccga gtcggacgtg cggaagacgg tgcggagctg cgtgttcgat
    30661 gagaatcgga atccgacatt agcgctgcct gtcgtgtccc ggtagtagtc attcgagaat
    30721 tcgccggtgg tgtggaactg ttcggcattc agggcgtagt cgaggcgggc tccgctgccg
    30781 cccagcaggt tcgccaccca acggtcggtc tggaaactgc cccgctcgta cgagagcgag
    30841 ccgcgcggta tcttgttttc tgggtctcca cgccgagtga acaattgcac aactccggcc
    30901 gaggcctccg cgccgaatag ggcgctttcc ggaccccgaa ccacctcaat ccggtcgata
    30961 tcgctcgagc tcaggtgcgc caggttgtac tccccgccgg ggtcgttggc aggcacgccg
    31021 tcgatcagaa tcatcgtgcc cgtgcgctgt ccgccgcggg tgaagaccga agtcaaagct
    31081 ccctgccgtc cggtcgtcgt cacctgcaac ccgggcgttt cacggagcag atccgggacc
    31141 gtcggaaacg ctcgccgctc cagatcagac cgggtgagga cagtggcggc gacccccgcc
    31201 tcctccgttg tcgtttcgta tcgggtggcg gagacgataa tccgctctac cactcccgcg
    31261 acggtcatgt cgacgcgaaa gtccccagga gcggcgatct ccacctcccg cacctcaaac
    31321 cctgatgccg tgatgacagc ccggcagcgt tcgccgccgg ggaagttgaa ccggccgtcc
    31381 aggccggtca ccaccgtctg tgagccgcat tcgacccgcg ctccgcggat cggtcgggcg
    31441 gacggatcgt ataccgtccc cgtgattacg agctgtgtag ggtctgccgc cactgtggcg
    31501 gccatagacg tgagtaagat gagacttcgg aaatatcctg ccatgagacg ttctccctcg
    31561 gaacgcatca ctcatctggt gaggaaccgg tcttctggct tgcgacctgg ccgttaccaa
    31621 tcccggttgc ctggccttcc cgctttcgca gtgaccaagc ggccgtgcag cacgtcgctt
    31681 acagttgcgg ggcagcggtg gacttacacc accttctcgt tcattcctcg cccgcgggtg
    31741 acacttgtga aagatcgaaa ttgccggccg ccccggcata gccggcgcat ctccaggcaa
    31801 ctttgctgag tatagcagtt cgccaggcta ctggatgtac tggatttcgg gcggctcgga
    31861 gagtggaaaa acagcctcat ccgcgtcttc ggtgtcgtcg aattttatcc ggcgaatctc
    31921 cagggcgatc cgcacgggtc cgccttcctc gctgacccgg actaccctcg ccagacagtt
    31981 cagaccccgg ccacgaatgt gttgcccgga aggcaggcgg actacaaccc gcgccaggtc
    32041 gcccaatctt ggtgatcgtt cggccggcgg ggcggaaatc gtcactagca gtccggagcg
    32101 gctgatattt tgagtgacgc caaccattgc gtcaaaagac ttcagcgggg aggataggtt
    32161 gcagtctagc ttgagttcat accggattcc ccggcgttga tccatggcag acatcaatgg
    32221 gtacccatag cagactagcg caacccaccc ggctggggga actctctttt ggacagttcg
    32281 cccttttgaa cgctctagta cttgcgtaaa cttggttaac atgttcaacg gagcccacta
    32341 ccccgcacag actgtccttg aagcctcgcg tacaatggtc tcaagtgctg ggcatcacga
    32401 agaaaaattg gaagaagtac tcgatccttg cgacgatcgt cttcgtcagc atcgtgatca
    32461 cgctgctgtt gggcgagttc cggttcttcc aacacctgca cctgaaggct ttggatgctc
    32521 actttgtggc ccgtgggcgt gagccgacct ccggtatcgt gttgctggta atcgaccaga
    32581 aggccctgga ccattttgag gaattgcagc ttttctggca tccgtattat gccgaagcga
    32641 tacgtggggt ggcggagggc ggtgcaaaag ttctgggcat ggacgttatc ttcgccgtgc
    32701 ctgtcacgaa gtgggaaccc gagcacgacc ggatgcttgc ggaagccgtg gccgatacgg
    32761 ccagccgcat gcccgtcatc tgcggctatg cgccggcagt gatgagcaaa cagcaggagt
    32821 ggccggtgcc ggtgaacatg atcgcgtcgg cgctgggcat gacggcgttt gccaatctca
    32881 cagccgatcc ggatgatttc gtgcggagac aggaactgat cgaaacgccg gttcccggga
    32941 gccaagtccc accggcacga agcctggcct taaggcttgc tgaagtgtat ttgggcacac
    33001 aagctgattt tgatggaaca cgcttcttgc tgggcggtgg tacggtgcca gtctcacgcg
    33061 atcgagccat cgcgatcaac tatgccggtc ctcccggtgc tttcccccgg atctcattgt
    33121 gggactttct ccaggccgct cgcgccgggc acaaagaaca gatacggcaa tgggttgaag
    33181 gaaaagtggt gctcctcggc cccgacaaca tcacggaccg ctacgccacc cctttctaca
    33241 ctgctttcag cgggccgcgc tggaccaccg ccggagtgga aattcacgcg aatactctcc
    33301 acacactgct gacgcgccgc ttcctgacgg caacgccgca agccggccga atgctggcgc
    33361 tggcggctgc aaccgggggc gccgtcgcca ttgttgcgtc attcagcgcc gccgcggcag
    33421 gactatggtt gactctactc ctcgccggcg tgatcgtctt tacccatatt ctgttccggt
    33481 ttggcgtgct gctctccact tcggaactcg tattgtcctg cgccttctgt gttctgttcg
    33541 ctgtcactta tcgctttctg acagcccaaa cacggggaga actgttttcg aaggctgtca
    33601 atttgttcat agggcggcaa ctcgctgttt ctctcgagga cacccaatcc atcacgctta
    33661 ccgcaaaaca cgcgtacgtc acgattctgt tctccgatat tcgcggcttc acggcctttt
    33721 ccgagtccaa ggaccctgaa gtggtcgtcg aacggctgaa cgagtatctg actgtgatgg
    33781 tgcgaatcat catgaatcac cacggccatg tcaacaagtt cctcggtgac gggattcttg
    33841 cggtcttcag cgatgacgat ggaaccaatc ctggagacca ccccgtgcga gccgtgcgat
    33901 gcggtaccga catgtgcctg gcgtcgtccg agtttcggac cggtgtcggt attcacactg
    33961 gtccggtcgt tatcggaaat gtgggctcga ctgatcggat ggagtatacg gtgctcggcg
    34021 atacggtcaa cctggcgtcc cgtcttgaaa gcctgaacaa ggagcatcat tcgcagttga
    34081 tcatgagtga ctccactaaa gagcgccttg acggacaggt tgaaaccgct cacctcgcat
    34141 cagtcgccat acgagggcgc gttaacaagc tggacatatt taccgtgtcc gcactggcgc
    34201 ccaatcaagg ggaacaggca gtcactcaga aggccccatg atccggggcc gcgctcggat
    34261 gatcgttccc gtcgtgagcc gctctacggc tcagtttcac ggacggatgg ccattgtcac
    34321 cggcgggctt tcctggcccc tgactcgcaa gacaaccggc acggcgtctc ccggagtcac
    34381 gcctgcagga acaatcgcgt tcaactggta gaggcccgcc cagcttggcg ccaggccggc
    34441 gaacagaacc tgagcctcaa cccccccgat cgtcaaggaa tactggccgt tcacccaaga
    34501 cagcggatca aacggggcag ccgagccagc cgtcactccc gggtctacca gacccagccc
    34561 gagacagtaa atcaccagga tgtcgccggt tttggccggg ttcgccgcat ccgcatacaa
    34621 gccgctggaa tgatgtacca cgacaccttg accacgcccg ttctggtccc tcgtgaggat
    34681 gccaggctgt gcggctgcca ccgtcacggt ctccggtgtg gcgatgcggc tgcctctctt
    34741 aaccacgagt tggtgcgtcg tgttcatcgg aacgtcgtaa gggacgatgg cgttgatctg
    34801 accctcactg gtgaagagca ggggcatgcg ccttccggca atggttgccg aggtgccggc
    34861 caactcgatc tccaagggca gcaccttcga aagctcgact cgatccgaga gccgggcgcc
    34921 gaaaatgctg acaaaggcgc ccggggccac cgggagcccg ggcgcgaaac tggcggcgct
    34981 tacgacgccc ccggcgaaga tcaccggcgg ctcgtcgccc ccgggacgca gggcggcggt
    35041 aaactccgca gtgccttcga tgttcttctg aggttgactg gccttgagtc gaatcgtgac
    35101 ctgcggcgat ccggcggagg taacctgcca gatcccgctc cactttccat tctgctgcga
    35161 ggttagcgcc aggggcggat cgttattgga gaactccgcg acgaccaatc catccaccat
    35221 ggggttccca cagtcatcca ccgcttccat cgccagccgg ttcggccagc ccgcgagaac
    35281 ggccgtttcc aatccaacag acgtaaacac gggtaatagt ttcgccggcg tgcatggagc
    35341 ctcggcaaac gcagcgccgg ccctcgcggc tgcgggagcg ggtgcgttta ccaacagaag
    35401 attcacagtt cgcgacacgc cgccggtgaa aagcagcgtc aacgagcctt cgtaaatgcc
    35461 gggagcaagt ttcgccgtgt ccggttgcac tacaatccgc gtcgggcgct caggagttac
    35521 cgttgcatcg gacggaagat agacgaacca gtccgtaccg cctgagctca ctcgcgccga
    35581 gcggaaactg atcgggttgt cgctcaggtt ggaaaccagg atgttctggg aactgggtgc
    35641 aacaccctgc gtggcgcgga aaacgagacc aagagggtgc accacgggac ctggatcggt
    35701 ggcgtcaggc agcagtttca gcgccactgc gacggactgg ggcgagttca cggcctgcgg
    35761 cgagctgatg tcgatctgcc cgtagtaatc acctgcggcg agaccggtcg ggtttatccg
    35821 aacctcgacc gtggaaccgc tgaatgagcc ggctgcggag ccgctgtccg cgcttaacga
    35881 aagccatggc acgcctccgg acaaccgggt caccttcgcg ctccaggtca gttctcctgt
    35941 ccccgcgttg agaacctgga agctctgcgc tggaacggct cctccatttt gcaccgctgc
    36001 gaatgccaac cctgccggag acagcaccat ccgttgcggc accgcgttca ggctcatcac
    36061 cacgggcacc gtcacctgtt ctccattctg cgtgttcgtt aggacgatct tgcctgtata
    36121 cgtgccgctg ctcagccctg aaggatcggc ccgcacgtcg attgacgcgc ccgtggaggc
    36181 cggcgcagcg gcggattgag gtgctgcgct cagccagcct ccgccgctcg ttgtcgtggc
    36241 ggcgacagtg aattccagtg tcccgcctcc tatgttcgaa acatcgaggg tcttcaggga
    36301 agcagcggac ccttcaatga gagagaacga caaggcgtcc ggccgggcag ccagccgcgc
    36361 cgggagcgtt gggccaacct gaaattccac cgcgacaagg acgaatggtg gggtgcactg
    36421 aggggcctgc aacgtgaccg tgccacgata gcgcccggga ggaagctccc cgggtttgac
    36481 ggagacctcg actgtcgccg gcatggtccc atctttcgga gtgaccgcaa gccaggttcc
    36541 tcctgattct gtggccacgg tgacgctaaa cgttacgccc ggtactcccg ggaacagccg
    36601 aacgcgctgg gacggcggag ccgcgccgag agagtttccg gcaaagctca actgcacggg
    36661 ctcgaccaga agggcgggcc gggtggcttt gatctgcctc aggcgatggt taaaacggtc
    36721 ggcgaagaag atattgccgg cggaatcgac cgccagcgaa tacggcccat agagggctgc
    36781 ctgcaccgcc ggctggccgt caccggacag gccctgcacc ccgttccctg ccacagtggt
    36841 aatgttgccg cccgaagaaa tgcatcggat gcgatggttc ttctcgtcgg cgacgcaaag
    36901 atttccggcg ttatcaaagg cgaggccgac aggtccgttc aaggacgctc tggtagctgg
    36961 cccgccgtca ccggagaaac ctgccgtgcc cgtccccgca atcgtggcga tgctgccata
    37021 gatgtcgatt ctgcgaatgc ggttgtttcc agtgtcgctg atatagatgt tccccgagtt
    37081 atcgacggcc aggccacgag ggtcggacag ggcggctctg actgccggcc cgttgtcgcc
    37141 cgaaaacccc ctttcgccag tgccagcgat agtggtgatg gttccgtgga tatctaccat
    37201 tcgaattcgt tgattgctgg tgtcggcaat atagagcgtg cctccagcgg cggcgagtcc
    37261 ttccggaaac ttcaaggtgg cgttcacagc gggtccgttg tcacccgcaa aaccggcttg
    37321 cccgttcccg gcgacagtgg cgatgttccc gtcaggcgtg actctacgga cgcgactatt
    37381 gaactgatcg gcaatgaaga gggagcccag ctcctcgtcg aaggcgattg ccgaaggata
    37441 gaacagggcc gccttcgagg ctggaccgcc atcgcccgag aagccttcct cgccggttcc
    37501 gacgaacgtg gtaagtattc ctttggaaat cttccggacg cggttccgaa atggttccga
    37561 caaataaagg tttcccacag ggtccacggc ggtgcccgtc ggaagataca gagcggcggc
    37621 agtcgccggt ccaccctcgc ccgacgtacg aaagaggccg ttacccgcta cggtcgagat
    37681 cacgccgtcg ggtgttacct tgcgaacgcg gctgttggcc gaatcggcaa tgtagacatt
    37741 gagtgacttg tcgatcgcaa cgcttgacgg ccagtttagc cgcgcccttg tcgccgggcc
    37801 gccgtcgccg gagtacccgc tttgcccact gcctgccact gtcgtaataa cgctgcctac
    37861 gaccttgcgg acgcggttcg tgaggagatc cgcgatgtat aacgcgcctg agctatcgac
    37921 cttggttgaa aggggcacca tcacggagaa aacgccgtct cccggggctg acccgccggc
    37981 caccgtagtg atggttccat tcggcgaaat acggcgaacg cggccgttga aggtgtcgga
    38041 aacgtaaatg ttgttggcgg catcgacgga cacgccgtag ggcgtactca ggagggcttg
    38101 cgtggcgggt cccccgtctc ccccaaagcc ctgcctgccg actcccgcaa tcgtggtgac
    38161 aataccatcc ggcgtgatct tgcgaatccg gtgattgaag gtatcagcaa cgtagatgac
    38221 tcccgccgcg tcaacagcca ggtcctgagg aaagttgaag gcagctccgg tggccggtcc
    38281 tccgtcaccg gagaacccgg gatcactcag gccggcgata gtgctgatga cgccgccgcg
    38341 gctgatcttg cgtatgcggt tcgcggcggc gacataaaga tcgctcgcag gactgaacgc
    38401 cagggcggcc gggaataaga gggctgccga ggtggcggcg ccctcatcgc cagagtagcc
    38461 ggggatgcca ttgccgccga ttacggtcag gacgccgtcg gatccgaccc gcattaccat
    38521 ttggttgtcg ggatcggcga tgtagaagtc gccggcggcg tcgacggcca ccgccatgct
    38581 gaagttccca ccgaggggcg cttcccgcgc cgggcgcccg tcacccggga acagccaatc
    38641 cgtgcctgcc gccgtagtaa tgaccccttg agcgccggct cgcgatacgc caaaaccagc
    38701 gatcgacagg aaagccagaa aaaggcgaca ccatcccctc acttttcgca tccccattgc
    38761 gaaaaagtat atcatctgtg gcgcttcagc ttgccgccga tccgtttccg ggtctcaccg
    38821 tccggggcga agcgcagtgc ggcgctataa gcgtcctttg cggcctgctc acggccgcgg
    38881 gccgcctcca agtctccgat tgcctcgtgg acttgcgatg catgcggctg tcgcttgagg
    38941 gcctcgttca actggtccag cgcggcctcc ggttggttgt tcttccgaaa caggtcggcc
    39001 gccgccagcc gtgcggcgac gtagtccggt ttcagcctga ccaccgtttg gtattggcgg
    39061 atggcttcct cggtatcacc tgcgcgcgtg agagcccggc ctaggctgag aagcgtcggg
    39121 aggtactccg ggtctcggag gagattgacg cgccacaact ggatcgcttc cgcaagcccc
    39181 tccgcacgtt cggacaacag aacggccagg ttatgacgcg ctgacagtag ctggggatcc
    39241 ttctccaggg ctgtccggta aagccgctct gcttcggcac ggcggccggc ggaagccttc
    39301 aggtaaccaa gggcattgta gggctccgcg cgctctggtg tcagtgcgat tgctttgcgg
    39361 taagcccctt ccgcttcctt ccggcggttg aggcgctggt agagcagacc gaggttatac
    39421 ggcaggtagg agtaggcagg cgccaacgtc atcgcgcggc ggtaggtctt gatcgcctcg
    39481 tcgtacttgc ccgcctccgt gtgcgtcagc gcgaggttat gtagcgggta tgcccaatag
    39541 ggcgctagcc ggatggcatc ctgaaacgcg gcgattgcgc gatcgtaggc agcctgctcg
    39601 aggtaggaga ttccgagtgc gttgtacgaa tacgcacccg tgggatcgaa acgaatcgca
    39661 cgttcaagca gctccgtcgc ttcctgatac cgcttgtcga acagcgcggt acggcctctg
    39721 caaaacactt cccggctccg caaaaggagt gattcaggcg tgaggcggag agcggcgccg
    39781 aaccaattgg tgccggcggc gaaatcggct cgtgtttgtg gaacctggtc tccggtgagg
    39841 tagcgaagga gcacttgctg cccccggtcc tccaaatcga ctcgcagctt gttctccgcg
    39901 agcaaatact cctccggaga ctgggttttc ctgagctggc ctagtatgtc aaaagctcct
    39961 cggggctcgc cgggcaacac tttgcccgcg gcaatggcgg cctcgagatt gggcctgcct
    40021 tccggcggca ggtcgaggga tcgcgtcatc cccggcggtc tcgcttgggc caaaagcagc
    40081 ggctcctgtg gcaagccggt ccacttcgcg atttcgatgc cttcgcgccg gacatccgca
    40141 acggcggcct tggtttccat ggaaccgaag tcgcgcgggt gttgccggtc gtgtgttcct
    40201 tcggcaacct ttgctcgcac gtcctcgatt agctcgttca gatcgacaat gttgtccccg
    40261 tttcggtccg cttcaccgtt caaggcgcca agcaggaagt aggagaaggc cccgtggccg
    40321 ccgccgaatt ccggcccctc ataggaaact tctttggccc ggctggcagt gaagagaaac
    40381 atctcccctt ccgcttcttg aagccgctcg acgctgctgt tgacgctgac ggtgcggctg
    40441 gcgatagtgc cgaccgtggc cgcacggcag gcgtcgatgt aagccatcac ccggccgacg
    40501 cgtgaaaggt cctcgcgcag gagcttatgc acgtctgcca tgggaagagc ggttgcagcc
    40561 aggtcctggg gatcgctgtc ataggtcacc aggaaggcgc cgcgctccgg ctccgccacc
    40621 ccgtgcgcgg cgatgagcag gatcacggta tcctttgggc ccgctcgtac tttcaggaat
    40681 gtctcgaacg cgtttcgaat ggcagcggtt gtggcggcct cgttggttag caccaccacg
    40741 tccgagtccg gcaatgcgcc gccccgtgga ctcttcagat attgcgcgaa gacacgcgcg
    40801 tcccggtgcg cgaagcggag ttgggggacc tgttccgagc gatagcttga gattccgatc
    40861 agcagcgcgt aggtcttgcc ttgcgcgggg cgttcggcga ggcgctcatt ctccagatcg
    40921 aacaagcggc tgctgatcca agccgcgtcg ggccaaactt tggccagctt ccggtactcc
    40981 tcggccgcct ctggcttcag a
//
LOCUS       DATIFQ010000140         2781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1772103, whole genome shotgun sequence.
ACCESSION   DATIFQ010000140 DATIFQ010000000
VERSION     DATIFQ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772103"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="VLE22_06575"
     CDS             complement(<1..1608)
                     /locus_tag="VLE22_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HSB14105.1"
                     /translation="MVRRLCIGLLWLAVVLSGAVPSLEEHFGYTPGDDYKLAGYGEII
                     GYFQKLERASGRIRLVEFGRSSHGKPMYAAFLSSEQNLRNLDRYREISRRLALGQAEP
                     EEARRLAGAGKAIVWIDSGLHSTEVAPSQHAPHLAWRMVTGETEEIRRIREKVILIQV
                     PCINPDGLDWVVEWYRGNLGSPYELARLPRLYQKYAGHDNNRDWFMMNLDETRHVSRL
                     LFREWFPQIVYNQHQTPAFPARIFVPPYSDPLNPNIPSAVMEGIHLIGAAMKERLARE
                     SRAGVLSYNQFDAWWNGGLRTAPAFHNMHGILTETALFLYATPREYKKSEIPERFASG
                     ISAREPSVFYQRPWMGGKWTLRDAIDYMLTADFAILDLAASRSPEFLMKAYDLARASI
                     EQGRKGSPYAYVLSPEQWDRSTVAEFLWRLSRAGIEVRRAVAEFTAGGKMYPKGTWVL
                     LASQAFRPYLVDLLERQKYPDLKTASGTSKRPYDVAGWTLSMQMGVAVDRIDAPFGAE
                     LELQNEIAIPPPSLDHRENSSFVATAEL"
     gene            1607..2518
                     /locus_tag="VLE22_06580"
     CDS             1607..2518
                     /locus_tag="VLE22_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HSB14106.1"
                     /translation="MWNNLTMATSDDRPFRVGLVQMACSHDPNENLAKAEWRIREAAG
                     LGAQIICLQELFRSLYFCQEENAGMFALAEPVPGPTTATLSKLAAELGVVVVASLFER
                     RAPGLFHNTAAVLDADGRLLGVYRKMHIPDDPQYYEKFYFTPGDLGVPVFDTRFARIA
                     VLVCWDQWYPEAARLASLGGPSVLFYPTAIGWHPSEKQEHGEGQLDAWRTIQRGHAIA
                     NGLFVAAVNRVGYEGSTQQGLEFWGNSFVADPFGKLVAEAPRDRETTLIAECDPARVD
                     EVRCQWPFLRDRRIDAYAPILNRWLDR"
     gene            complement(2584..>2781)
                     /locus_tag="VLE22_06585"
     CDS             complement(2584..>2781)
                     /locus_tag="VLE22_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14107.1"
                     /translation="NAQDAILFLNANQQGLWKPEYGKYTRNMVAEAGQIATSVHEFET
                     YASARQTEMQLGKDLGMSSGS"
BASE COUNT          519 a          873 c          867 g          522 t
ORIGIN      
        1 cagctcggca gtagcgacaa aagaggagtt ctcccgatga tccaaagacg gcggcggaat
       61 tgcgatctcg ttctgaagct cgagttccgc gccgaaaggc gcgtcaatcc ggtcgacggc
      121 cacgcccatc tgcatgctca gagtccaacc ggcaacgtca taaggacgtt tcgacgttcc
      181 cgaggccgtc ttgagatcgg ggtacttctg ccgttctagc agatccacca ggtacggccg
      241 gaacgcttgc gaggcgagga gtacccatgt gcccttcggg tacatctttc cacccgccgt
      301 aaactccgcc accgcccgcc ttacttcgat gccagcgcga gagagacgcc acagaaactc
      361 cgccaccgtc gatcggtccc actgttccgg agagagcacg tacgcgtaag gcgatccttt
      421 gcgaccctgc tcgattgacg ctcgggccag gtcgtacgcc ttcatcagga attccggcga
      481 ccggctcgcg gcaaggtcga ggatcgcaaa gtcggccgtt agcatgtagt cgatggcgtc
      541 gcgcagcgtc cactttccgc ccatccaggg acgctggtag aagacgcttg gctctcgggc
      601 ggagatgccg ctagcaaagc gctcgggaat ctccgatttc ttgtactcac gcggagtcgc
      661 gtacaggaat agcgccgtct ccgtgagaat cccgtgcatg ttgtgaaacg cgggggcggt
      721 ccggagaccg ccgttccacc aggcgtcgaa ctggttgtag gagagcacgc ctgccctgct
      781 ctcgcgggcg agccgctcct tcatggcggc gccgatcagg tgtatgcctt ccatcaccgc
      841 ggaagggatg ttcggattca ggggatccga gtacggaggg acgaaaatgc gggccgggaa
      901 cgccggagtc tggtgctggt tatagacgat ctgcggaaac cactcgcgaa acagcagacg
      961 cgagacgtgt cgtgtctcgt cgaggttcat catgaaccag tcgcggttat tgtcgtggcc
     1021 ggcgtacttc tgatacagac gaggcaggcg cgcgagctcg tacggtgagc cgaggttccc
     1081 tcggtaccac tccaccaccc aatccaggcc gtcggggttg atgcacggca cctgaataag
     1141 gatcaccttc tcgcggatcc ggcgaatctc ttcagtctcc ccggtgacca tccgccacgc
     1201 gaggtgaggc gcgtgctgcg agggggccac ctcggttgaa tggaggccgg agtcgatcca
     1261 aacaatcgcc ttccccgcgc cggcgaggcg gcgcgcctct tcgggttccg cttgtccgag
     1321 ggccaggcgg cggctgatct ctcgatagcg gtccagattc cgcaggttct gctccgagga
     1381 cagaaacgca gcgtacatcg gctttccgtg cgaactccgg ccgaattcga cgaggcggat
     1441 gcgcccgctg gcacgctcga gtttctggaa gtagccgatg atctcgccgt acccggcgag
     1501 cttgtagtcg tcgcccggcg tgtagccaaa atgctcctcg agcgacggga ccgcgcccga
     1561 gagaaccacg gcgagccaaa gcaaaccgat acaaaggcgc cggaccatgt ggaacaatct
     1621 tactatggcg acctcggacg accgcccttt ccgcgtgggt ctggtgcaga tggcctgctc
     1681 ccacgatccg aacgagaacc tcgccaaggc ggagtggagg atccgggaag cagcgggcct
     1741 cggagcacaa atcatctgcc ttcaggagct gttcaggtcc ctgtactttt gccaggaaga
     1801 gaacgctggg atgttcgcgc tggcggagcc agttcccggg ccgaccacag ccacgctctc
     1861 gaaactggcc gctgaactcg gcgtggtcgt ggtggcatcc ctgtttgaac ggcgggcgcc
     1921 cggcctgttt cacaatacgg cggcggtact cgatgctgac ggaaggttgc tgggcgttta
     1981 tcgcaagatg cacattcccg acgaccccca gtactacgag aagttctact tcacaccggg
     2041 cgatcttggc gttcccgttt tcgacactcg cttcgccagg atcgccgtgc tggtctgctg
     2101 ggaccagtgg tatccggaag cagctcggct ggcgagcctc ggtggcccga gtgtgctttt
     2161 ttacccgacc gccatcggat ggcacccgtc cgagaaacaa gagcacggcg agggacaact
     2221 cgatgcatgg cgcaccatcc agcgcggcca cgcgatcgca aacggccttt tcgtcgctgc
     2281 cgtcaatcgt gtcggttatg aaggttccac gcagcagggt ctcgagttct ggggtaactc
     2341 gttcgtggcc gaccccttcg gtaagctcgt agccgaagcg ccacgagacc gggaaacgac
     2401 actgatcgcc gagtgcgacc ccgcgcgggt cgacgaggtt cgttgccagt ggcccttcct
     2461 ccgggaccgc cgcatcgacg cttacgcgcc gattctcaac cgctggctgg accgttgagc
     2521 cgcacaaaaa aagggtcgcg ggaggacggg ggcctcctcc cgcgacgaag gaaatcgcgt
     2581 ctgtcaggac ccgctgctca tccccaggtc cttcccgagt tgcatctccg tctggcgggc
     2641 actggcgtaa gtctcgaact cgtgtacgga cgttgcgatc tgacccgcct ccgcgaccat
     2701 attccgggtg tacttcccgt actccggctt ccacaggccc tgttggttag cgttcagaaa
     2761 gagaatcgcg tcctgggcgt t
//
LOCUS       DATIFQ010000141         6336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_48475, whole genome shotgun sequence.
ACCESSION   DATIFQ010000141 DATIFQ010000000
VERSION     DATIFQ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48475"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..710
                     /locus_tag="VLE22_06590"
     CDS             234..710
                     /locus_tag="VLE22_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14108.1"
                     /translation="MVSMTYQPTNPLTGQGTVGTAPFRYLPADEHRVRPRQVETDPLS
                     KTELFLSQEIAVFFSDALRSELSRSGYTINDSSDRIVSGVITRFFVDWKSREGRLFEL
                     AADYTVQSAERTIFSWHCSSIQKGPNTLAEDGILIRKGIADCMHRFIAAAQEASAF"
     gene            1035..2336
                     /locus_tag="VLE22_06595"
     CDS             1035..2336
                     /locus_tag="VLE22_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB14109.1"
                     /translation="MRRMNYILLSIAVLVTAGCVTSGTHEQTVAELDQARKATSKMAT
                     DFDNFKKQSAAQIAALEADKTKLSNDLLAAQTASGKIQRELDQVKNNLDSTLASRKAL
                     EDDVRKIRAETSGMERLNGELRREREQLQDKLDMLQKNYDATKQDLETKLKAEQETVA
                     AVQKDKELLIARTMGATDELAKAQKRLGEIETDAARAKDLEQRLGELQKKTGELETDA
                     SRAKELEQRLSQRDVELGKLAQGGETLAAEKQALAAEKERLEKERAAKEAEIARLTKT
                     QEDLSKSLQAQIEKGDIKIKQVRDRLTINMVEKVLFNSGQAQVKPEGLNVLKQVADVL
                     KTVNDKQIRIEGHTDNVPIGVKLQQKFPTNWELSTARATNVVRYFIEKGNLNRESLEA
                     VGYADTRPVAGNDTEAGRTENRRIEIVLYPKDLGSIVGEIK"
     gene            2513..3430
                     /locus_tag="VLE22_06600"
     CDS             2513..3430
                     /locus_tag="VLE22_06600"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HSB14110.1"
                     /translation="MIDRHAVRHSAARPLAAAVLALLTIVVPFGIAITWANGELREAL
                     RNMTYPSDWTRGGRASLVDGIYEEPAAPGSASKIVVRLTDSVAVGKIGDQPVAALVIV
                     TDPGGSGVFFDLYLAQQRENRWLVLDRAYLGDRIRVHAIEIAQGLISLDLTVHGPRDG
                     ACCPTERSRVSFALQDGRLVTVNAPVHAAGNSIEGQVWAWQHTTRVDGTRTTPTEPEH
                     FTIRLGPDGQLAVRADCNRAGGTYRLEESHLLLSVTHSTMAACPPESLDRQFLADVAA
                     VTAWRLEESRLHVDLGSGGTMVFKKSKGF"
     gene            3433..5076
                     /gene="pgi"
                     /locus_tag="VLE22_06605"
     CDS             3433..5076
                     /gene="pgi"
                     /locus_tag="VLE22_06605"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HSB14111.1"
                     /translation="MAMPIAPLPERPAWKALTAHHRTNRNLHLRKLFADDPARGERMT
                     AEAAGLYLDYSKNRVTDKTLTLLLQLAEESGLRERIDAMFRGEKINVTENRAVLHVAL
                     RAPRGAMLSVEGEDVVPRVHAVLDKMTDFCNRVRSGAWKGHTGKRIRNIANIGIGGSD
                     LGPVMAYEALKHYSNRDMTFRFVSNIDGTDLMEAVRDLDPSETLFIVSSKTFTTLETM
                     TNAHSARAWLLAGLGGDAKSVVKHFVAVSTNAAEVTKFGIDTANMFEFWDWVGGRYSM
                     DSAIGLSTMLAVGPDHFRALLSGFHEMDEHFRTAPFAKNLPVLMGLLAIWNGNFLGAE
                     TVAVLPYEQYLKRFPAYLQQLTMESNGKRVTLDGKEVAYQTGPVYWGEPGTNGQHSFY
                     QLIHQGTKLIPCDFIAFMQSLNPSGRHHDMLLANVFAQGEALAFGKTPEEVKAEGSPD
                     WLVPHRTFEGNRPSNTILLERLTPAALGKLVALYEHSVFTQGAMWNIDSFDQWGVELG
                     KVLAQRIVPELESNPEPRLGHDSSTNNLIRRYREHRKSS"
     gene            5130..6146
                     /gene="gnd"
                     /locus_tag="VLE22_06610"
     CDS             5130..6146
                     /gene="gnd"
                     /locus_tag="VLE22_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HSB14112.1"
                     /translation="MQLGMIGLGRMGANMVRRLLKAGHQCVVFDRSSDAVQAIVREKA
                     AGASSLADLVKKLDKPRAVWLMVPAAVVDQTIADLLPALEPGDILIDGGNSYYVDDIR
                     RAKALAAKRLHYIDVGTSGGVWGLDRGYCMMIGGESDVVKRLDPIFKSLAPGAGDIAR
                     TPGRENSTGTAEQGYLHCGPNGAGHFVKMVHNGIEYGLMAAYAEGFGVLRAANIGKQD
                     HAIDAETTPLRDPEHYRYDFNLPDIAEVWRRGSVVASWLLDLTASALSKDSALAQFSG
                     QVSDSGEGRWTIKAAIDEAVPAPVLTTALYQRFNSRGEADFQNKLLSALRYEFGGHHE
                     KPAK"
BASE COUNT         1344 a         1855 c         1967 g         1170 t
ORIGIN      
        1 atcctgcgtt gtgcctatgg cttgagtgtg gagctaacta ccacgcgcgg tagaagaccc
       61 ggctgcggat gtgtagaatg gcccccaccg caacgcaccg ttcccacatc cattgaaagg
      121 cttgtgcgat gatcatagaa ctccccgccg ggaaccgctt tcctttaggc aattcccgcc
      181 tatgggcgat cggacttctc ctcctcgttg tcaccacgac gggatgtccc cgcatggtgt
      241 ccatgacgta tcagccgacc aatccgctta cagggcaagg aacagtcggg actgctccgt
      301 tccggtatct gccggccgat gagcatcgtg tgcgtccgcg ccaggtcgaa accgacccgc
      361 tgtcaaagac cgaactgttc ctctcgcagg aaatcgctgt ctttttctcg gatgcgctcc
      421 ggtcggaact gagccggtcc gggtacacaa taaacgattc gagcgaccgc attgtgtccg
      481 gtgttatcac gcggttcttt gtcgactgga agagccggga aggccgtttg ttcgaactgg
      541 ccgcagacta tacagttcag tcggccgagc gcaccatctt ttcctggcac tgctcctcga
      601 ttcagaaggg accgaacacg ctggctgaag acgggatcct gattcgcaag gggatcgccg
      661 actgcatgca ccggtttatt gcggccgcgc aggaggccag cgctttctag aagctatctc
      721 aaaattgtgc ctctggcatt ccgatacgct ggttccttcc ccctcctttg taaggagggg
      781 aaggggaggt cgaggctctt gatggccacc atggccatgt tcaaccaaca tgaatatgag
      841 aggccacccg aggctctact catcccagcc tcccccattt tcctggatgt gcgattttga
      901 gattggtact agtcaaggaa gcgagtcttg cagcggtaag acaaataaat gccgcggctc
      961 ggacggaccc acggttgccg ttcttcgaca gctttgctat gaatagccac ggactaataa
     1021 gaggagggtt cgctatgaga cgcatgaact acatactttt aagcattgct gttctggtca
     1081 cggccggttg cgtgacgtcg ggtacgcatg agcaaaccgt ggcggaactg gatcaggcgc
     1141 gcaaggcgac ctcgaaaatg gcgacggatt tcgacaattt caagaaacag tcggcagctc
     1201 agattgcggc ccttgaagcg gataaaacaa aactctcgaa cgatctgctt gccgctcaga
     1261 cggcgagcgg gaagatccag cgggagctcg accaagtgaa aaacaacctc gactcgactc
     1321 tcgccagccg gaaggcgctg gaagacgatg tccggaagat ccgggccgaa acgtcgggga
     1381 tggagcggct gaacggggaa cttcgccggg agcgcgaaca actccaagac aagctggata
     1441 tgcttcaaaa gaactacgat gccaccaagc aggacctcga aacaaagtta aaggccgaac
     1501 aggagacggt tgcagcggtt cagaaagaca aggaactcct gattgcgaga acgatgggcg
     1561 cgacggatga actggccaaa gcgcagaagc gtttgggtga aattgaaacc gacgcggccc
     1621 gtgccaagga tcttgaacaa cggctcgggg agcttcagaa gaagacaggt gagctggaaa
     1681 ccgacgcgtc gcgtgccaag gaacttgaac agcgcctctc gcaacgtgat gtcgagctcg
     1741 ggaaattggc acaaggcggt gaaactctgg ccgcggaaaa gcaagccctt gcggccgaaa
     1801 aggagcgact cgagaaggaa cgggctgcga aagaggcgga aatcgcgcgg ctcacgaaga
     1861 cgcaggagga tctaagcaag tcgctgcagg ctcagatcga gaaaggcgac atcaagatca
     1921 agcaggtgcg cgaccggctg acgatcaaca tggtcgagaa ggtgctcttc aactcgggtc
     1981 aggctcaggt gaagcccgaa ggcctgaatg tgttgaagca ggtagccgat gtcctcaaga
     2041 ctgtcaacga caagcagatc cggatcgaag gccataccga caacgtcccg atcggggtca
     2101 agctccagca gaaattcccg accaattggg agctgtcgac ggcgcgggca accaacgtgg
     2161 tgaggtattt catcgaaaag ggcaatttga accgggagag cctcgaggcg gtgggctatg
     2221 cggatacgag acctgtggcg ggaaatgata ccgaggcggg ccgcacggaa aatcgccgca
     2281 ttgaaatcgt gctctacccg aaggatctcg ggtccattgt cggcgagatc aagtagtcat
     2341 ctcagtatgc cagcgcccgc cgcgaccgcg gcgggcgcct ctccacatca ttccccaagt
     2401 tcccgggtcg tgcaggatcg ccgaagcagc ctgactcaat tcgcgctcga accggcctga
     2461 tccaggtgga tgcggccgat caagtcctgg tcgaactgcg ggagagcatg aagtgatcga
     2521 tcgacatgcg gtccgccatt ccgccgccag gccccttgcg gcagccgtgc tcgccctgtt
     2581 gacgatcgtt gttccgttcg gaatcgcgat cacatgggcg aatggcgaac ttcgcgaagc
     2641 cctgcgcaac atgacctatc cctcggactg gacgcgcggc ggccgggcat cactcgtcga
     2701 tggaatctat gaagaaccgg cagctccggg ctcggcctct aagattgtgg tccgattgac
     2761 cgattctgta gcggttggga aaatcggcga tcagccagtg gcggcgctgg tcatcgtgac
     2821 ggatcccggc ggcagcggag tctttttcga tctgtatctt gcgcagcagc gggagaatcg
     2881 ttggctcgtt ctggatcgcg catatctcgg cgatcgcatc cgcgtgcacg ccattgagat
     2941 cgcacagggt ctcatcagcc tcgacctgac cgtccatggt ccgcgcgatg gcgcctgctg
     3001 cccgaccgag cgcagcaggg tttcttttgc gctgcaggat ggccggctgg tgactgtcaa
     3061 tgcgcctgtt catgcagcag ggaactccat tgaagggcag gtctgggcgt ggcaacatac
     3121 cacccgcgtc gatgggacga ggacgacgcc gaccgagccc gaacacttta cgatccgcct
     3181 cggaccagat ggacaactcg ccgtccgggc cgactgcaac cgggccggag ggacctatcg
     3241 cctggaggaa tcgcacctgc tcctttccgt gacccattcg accatggccg cctgtccgcc
     3301 tgagtcgctg gacagacagt ttcttgccga cgtggcggcg gtcactgcct ggcgtctgga
     3361 ggagagccgt ctgcatgtgg atctcggtag cggcgggacg atggtgttta aaaaatcgaa
     3421 aggattctga agatggcgat gccgattgcc ccgctccccg aacgtccggc atggaaagct
     3481 ctgaccgcgc accatcgaac gaaccgcaac ctgcatctgc gcaagctgtt cgcggatgat
     3541 cccgcgcgcg gcgagcgcat gacggctgaa gcggcgggcc tctacctgga ctactcgaag
     3601 aaccgcgtca ccgacaaaac cctcacgctc ctgctgcaac tggccgagga atccggcttg
     3661 cgcgaacgaa tcgatgcgat gtttcgcggc gagaagatca atgtcaccga gaatcgcgcg
     3721 gtgctgcacg tggccctgcg cgctccgcga ggggcgatgc tcagcgttga aggcgaggac
     3781 gtcgtgcccc gggtccatgc ggtgctcgac aagatgaccg acttttgcaa ccgcgtccgg
     3841 agcggcgcgt ggaagggtca cacgggcaaa cgcatccgga atattgcgaa catcggcatc
     3901 ggcgggtccg acctgggacc ggtgatggcc tatgaggcgc tcaaacacta cagcaatcgc
     3961 gacatgacct tccgttttgt gtccaacatc gacggcaccg acttgatgga agcggtccgc
     4021 gaccttgatc cgtcggagac gctcttcatc gtgtcgtcaa aaacctttac gacgctggag
     4081 accatgacca acgcgcacag cgcgcgggcc tggttgctcg caggtctcgg gggtgacgcg
     4141 aagtccgtcg tgaagcattt cgtggcggtt tcgacgaatg cggccgaagt gaccaaattc
     4201 ggaatcgata ccgccaacat gtttgaattc tgggactggg tcggggggcg ctattccatg
     4261 gactcggcga tcggcctctc gacgatgctg gcagtcggcc cggatcactt tcgtgcgctc
     4321 ctctccggct tccacgaaat ggacgagcat ttccgcaccg ccccatttgc gaaaaacctg
     4381 ccggtgctta tgggtctact cgcgatctgg aacggcaatt tcttgggcgc ggagaccgtg
     4441 gcggtcctgc cgtatgagca gtacctgaag cgattcccgg cctatctgca gcaattgacg
     4501 atggaaagca acggtaaacg tgtcactctc gacggcaagg aggtcgcgta ccagaccggt
     4561 cccgtctact ggggcgagcc gggcaccaac ggccagcatt ccttttacca attgatccac
     4621 caggggacga aactcatccc gtgcgatttc atcgcgttca tgcagtcgct caacccttcc
     4681 ggccgccacc acgacatgct gctcgcaaac gtgttcgcac agggcgaagc gctggcgttc
     4741 gggaagacgc ccgaagaggt caaggctgag ggctcccccg actggctcgt gcctcatcgc
     4801 acgttcgagg gcaaccgtcc ttccaacacc attctgcttg aacggttgac tccggcggca
     4861 ctgggcaagc tcgtggccct gtacgagcat tccgtgttca cccagggggc catgtggaac
     4921 atcgactcgt tcgaccagtg gggagtcgaa ttgggcaagg tgctggccca gcgcattgtg
     4981 ccggaactcg agagcaatcc ggaacccagg ctcggccatg atagttcaac gaacaacctg
     5041 atccgtcgct accgggaaca cagaaagtct tcgtaattcc cgtaggagtt ttcagcacag
     5101 aacgatcagt ttaaaggagg aacctcatca tgcagctcgg gatgatcggc ctcggccgga
     5161 tgggcgcaaa catggtgcgg cggctgctca aagccggcca tcagtgcgtg gtcttcgaca
     5221 ggtcgtcgga cgccgtacag gcgatcgtca gggaaaaggc tgcgggggcc tcctcgctcg
     5281 ccgacctcgt gaaaaagctc gacaagccgc gcgcggtctg gctgatggtt ccggcggcgg
     5341 tcgtggacca gaccatcgct gacctcctgc ctgcgctcga accgggggac atcctcatcg
     5401 acggcggcaa ttcctactac gtcgatgata tccggcgcgc aaaggcgctt gcggcgaagc
     5461 gtctccatta tatagacgtg ggcaccagcg gcggggtctg gggcctggac cgcggctatt
     5521 gcatgatgat cggcggcgag agcgatgtgg tcaagcgtct cgatcccatc ttcaagtccc
     5581 tggctcccgg cgcgggtgat attgcccgca cgccgggacg ggagaacagc acgggcacgg
     5641 cggagcaggg ctatctgcac tgcgggccga acggcgccgg gcatttcgtg aaaatggtcc
     5701 acaacgggat cgagtacgga ctcatggcgg cctatgccga gggattcggc gtcctgcgcg
     5761 cggccaacat cggcaagcag gaccacgcca tcgatgctga aacgacgccg ctgcgcgacc
     5821 ccgagcatta ccggtatgac ttcaatctgc ctgacatcgc ggaggtatgg cggcgcggca
     5881 gcgtcgtcgc ctcctggctg ctggacctga ccgcgtcggc cttgagtaag gattcggcgc
     5941 tggcgcagtt ctccggtcaa gtgtcggatt ccggtgaggg acgctggacc atcaaggccg
     6001 ccatcgacga ggctgtgccg gcccccgtac tcacgacggc gctctaccag cgcttcaact
     6061 cacgcggcga ggcggatttt cagaataagc tgctctcggc cctgcgctat gaattcggcg
     6121 ggcaccacga aaaaccggcg aagtaagggg gaatccgccc tcccgatggc tggcacaatc
     6181 cggtcatcag cggtgaatca ggattgagga cggcttcatg aacatcgaag tgctggccga
     6241 cgccggagca gtcgcgcgaa aagctgccac tgtcattgcg gccgaggcgc aagcctccgt
     6301 ggccgcgcgc gggcgatttg tcatggccgt cagcgg
//
LOCUS       DATIFQ010000142         3224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1772774, whole genome shotgun sequence.
ACCESSION   DATIFQ010000142 DATIFQ010000000
VERSION     DATIFQ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772774"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VLE22_06615"
     CDS             complement(<1..524)
                     /locus_tag="VLE22_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSB14113.1"
                     /translation="MYAELHARSAFSFLEGASQPEELIEAAAGLGVPAVALFDRDSLS
                     GAPRFHMAAKKAGIRALIGAEITSVENCRYSLLAENRTGYQNLCRLITKMKLRAKKGE
                     AAATPEDLAEHAAGLICLTGGEEGPLALALRGGPEEGRRCAERLVSIFGKGNVYAEVQ
                     RHYDREEEARNQAVA"
     gene            complement(526..1809)
                     /locus_tag="VLE22_06620"
     CDS             complement(526..1809)
                     /locus_tag="VLE22_06620"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HSB14114.1"
                     /translation="MFACLYTPDEFAGPKLAELAGSFSPFVEMTAPGTVIFSIAGLGR
                     LFGTPHQIAAEIARRGAERGIAASLAIASNPDTAAVAARNLSGVTVIPPGEEAARLED
                     IPLERLAMPLELLETFDRWGMKTLGDVAALPPIGLAERLGEEGVRLHELSLGRAHRPL
                     RLAPPPTSYTEREELEDPVSQVEPLLFVLTRMLGEICGRLEAQSLATNRITLRLRLEN
                     RQEHTRILELPVPQRDTRTFLKLLELDLEAHPPPAAVTAAELTLDPAEPRTLQRGLFL
                     PLAPEPAKLQLTLARITAMVGEGNAGTPELLNTHRPDAFHLKKPGTVHSFPDFAELKS
                     GNECTVPGFVVRVFRPVLPASVRLRAETPHYVAAPGVRGEVSEAAGPWRTSGEWWTRT
                     RWARDEWDVELERNGLYRIYFDLDVREWFVEGVYD"
     gene            complement(1781..2431)
                     /locus_tag="VLE22_06625"
     CDS             complement(1781..2431)
                     /locus_tag="VLE22_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14115.1"
                     /translation="MSAAVPASTLLSISKAREEYLPTGLAQLDQLLEGLPRGAVTELT
                     GGRSSGKMSLLVSVLAQATERLEFCAVVDVHDVFDLSSAARSGVELSKLVWVRCRGNP
                     SIAMKAADLILHGGGFGVVCLDLSDVPPKVLQRIPISWWWRFRRAIENTPTVLLVLAG
                     QTQVKSCSSCWLDFRPGRAAWSGGKPALLLAGLNTEVVVRKPVKAETHVCLSLHAR"
     gene            2554..>3224
                     /locus_tag="VLE22_06630"
     CDS             2554..>3224
                     /locus_tag="VLE22_06630"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSB14116.1"
                     /translation="MTRPFLRYLLLTLAVCACASAEPMLTHVDVFTSGTEGYHSFRIP
                     AILTAADGSLLAFAEGRKENRTDPGGGDIDLVYKRSTDSGATWTPLKVLDDPGEKWAA
                     SNPAPVLDRSNGRIWIVYNRWEPGYGTDRSQTGTMNNQTWARYSGDHGQTWSAAIDIT
                     KNARDFDNWGAMFTGPGGAIQTRSSRLLIPAAMKPDTYSIWLSAGEFRGTMEFMRAYV
                     VYSDD"
BASE COUNT          583 a         1091 c          969 g          581 t
ORIGIN      
        1 gccaccgctt ggttgcgcgc ttcctcttcc cggtcgtaat gccgctggac ctcggcgtag
       61 acattgcctt ttccgaaaat tgacaccagc cgctccgcgc accgccggcc ctcttcggga
      121 ccgccgcgca gcgccagtgc cagtggccct tcctccccgc cggtgaggca gatcagcccg
      181 gcggcgtgct cggccagatc ctccggagtg gccgccgctt cgcccttctt tgcccgcagt
      241 ttcatcttcg tgatcagacg gcacaggttc tggtagccgg tgcggttttc agccagcagc
      301 gagtaccggc agttttccac cgacgtgatc tccgctccga tcagcgcgcg gatgcctgcc
      361 ttcttcgcgg ccatgtggaa ccgcggcgcc ccggacaggc tgtcccggtc gaacaatgcg
      421 accgctggaa cgcccaatcc ggctgcggcc tcgatgagtt cctccggctg cgaagcgccc
      481 tccagaaagc tgaaggctga gcgggcgtgg agttcggcgt acatgtcagt cgtagacccc
      541 ttcgacaaac cactcgcgga catccaggtc gaaatagatc cggtagagcc cgttgcgttc
      601 cagttccacg tcccattcgt cgcgcgccca gcgcgtccgg gtccaccatt ccccggaggt
      661 gcgccacgga ccggcggcct ccgaaacttc tccccgcaca cctggcgcgg ccacatagtg
      721 cggcgtctcc gcgcgcagcc ggacactcgc cggcagcacc ggccgaaaaa cacgaaccac
      781 aaaacccggg acagtacact catttcccga tttcagttcg gcaaaatcgg gaaatgagtg
      841 tactgtcccg ggttttttca ggtggaacgc gtcgggacgg tgcgtgttca gcagttccgg
      901 agtaccggcg ttgccttcgc cgaccatcgc cgtgatccgt gccagcgtga gttgcagctt
      961 cgccggctca ggcgcgagcg gtaggaaaag tcctcgctgc agcgtgcgcg gttccgccgg
     1021 gtcaagcgtg agttccgccg ccgtcaccgc ggccggcggc gggtgtgcct ccaggtccag
     1081 ttcgagcaat ttcagaaacg tccttgtatc ccgctgcggg accggcaatt ccaggatgcg
     1141 cgtgtgctcc tgccggtttt cgagacgaag gcgcagcgtg atccggttcg tcgccagcga
     1201 ctgtgcttcc agccgcccgc agatctcacc cagcatccgc gtgagcacaa agaggagcgg
     1261 ctccacttgc gagaccgggt cctccagttc ctcccgctcg gtataactgg tgggcggcgg
     1321 cgccaaccgg agggggcgat gcgcacggcc aagcgacagt tcgtgcagcc tcaccccctc
     1381 ctcgccgagc cgttccgcca gcccgatggg tggaagggcg gccacgtcgc cgagagtctt
     1441 catgccccac cggtcgaacg tttccaacag ttccagcggc atcgcgagcc gttccagcgg
     1501 gatgtcctcc agccgcgctg cctcctcccc gggcgggatc acggtcaccc cgctgaggtt
     1561 ccgcgctgcg accgcggcgg tgtccggatt ggacgcaatc gccagactgg ctgcgatccc
     1621 tcgctcggcg ccgcgccgcg cgatttccgc cgcgatttga tgcggtgtac caaacagccg
     1681 ccccagtccc gcgatcgaaa aaatcaccgt acccggcgcg gtcatctcca cgaagggtga
     1741 gaacgaaccg gccagttcgg ccagtttcgg accggcaaac tcatcgggcg tgtagagaca
     1801 ggcaaacatg ggtttccgct ttcactggtt tgcggacgac gacctcggta ttcagccccg
     1861 caagcagcag ggcgggcttc ccgccggacc aggcggccct acccggccga aaatccagcc
     1921 agcacgaaga gcaggacttt acctgggtct gtccggccaa aacgagcaga acggtgggcg
     1981 tgttttcgat ggcgcgccgg aagcgccacc accacgagat tgggatgcgc tgaagaacct
     2041 tcggagggac atccgacaga tccaggcaga ccacgccgaa tcccccgccg tggaggatca
     2101 agtcggccgc cttcatggcg atcgacggat tgccccggca tcgcacccag acgagcttgc
     2161 tcagttcgac gccggagcgg gccgccgagg acaggtcgaa gacatcgtgg acgtccacta
     2221 cggcgcagaa ttccagccgt tccgtcgcct gtgccagcac ggacacgagc aagctcattt
     2281 tgccggacga ccgtccccca gtcaactccg tgaccgcacc ccgggggagt ccctcaagta
     2341 attgatctaa ttgagctaat ccagttggaa gatactcctc ccgagccttc gaaatggaca
     2401 gaagcgtgga tgccgggacc gctgcggaca tagtctattt tcgcttttta ttcgccttga
     2461 ctattgtgtc ccgaaccttg gtctctgtca atcgggccgt tccagtgaat ggttgaaaat
     2521 cagccattcg atggttgaaa atcaaccatc gccatgacgc gcccttttct tcggtacctt
     2581 cttctcacgc tcgcggtttg tgcctgtgct tcggcggaac cgatgctgac ccacgtcgac
     2641 gtgttcacct cgggaacgga gggctaccac agcttccgga ttccggcgat tctcaccgcc
     2701 gctgacggct cgctgctggc gttcgccgaa ggacgcaaag agaaccgcac agatccgggc
     2761 ggcggcgata tcgacctcgt gtacaaacgc agcacggata gcggcgccac gtggactccg
     2821 ctgaaggtcc tcgacgatcc aggcgagaag tgggcggcct cgaacccggc tcccgtgctc
     2881 gaccgctcga acggtcgtat ctggatcgtc tacaaccgct gggaacccgg ctatggcacc
     2941 gaccgttccc agaccggcac gatgaacaac cagacgtggg ctcgctacag cggcgatcac
     3001 ggccagacgt ggtccgccgc gatcgacatc acgaagaacg ctcgcgactt cgataactgg
     3061 ggcgccatgt tcaccggacc gggcggtgcg atccagaccc ggagcagccg tcttctgatt
     3121 ccggcggcca tgaagccgga cacatactcg atctggcttt ccgccggtga attccggggc
     3181 accatggagt tcatgcgcgc atacgtggtt tacagcgacg acca
//
LOCUS       DATIFQ010000143        16822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_432705, whole genome shotgun sequence.
ACCESSION   DATIFQ010000143 DATIFQ010000000
VERSION     DATIFQ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16822
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432705"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VLE22_06635"
     CDS             <1..687
                     /locus_tag="VLE22_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14117.1"
                     /translation="GQQLRYYPSVRETIRKIHEEKVAGQTFVVKAQRDSAPSRKGPSN
                     RPSWYDDVKRSGDLIVENAVHNIDACNWIVGSRPVSAYGHGKKYLPQPIPAGTIMMDG
                     FSVEYIYENDVHVDYSQLAFHARGLKELPNGMWYTVFGDKGSVFMTHESAVLYDLYGE
                     SEAKDLLTTELKEGKEDAVAQFFACIREKRQPFADVQVAAIAALTAIMGREAIYRRRM
                     VTWKELGVEV"
     gene            699..3143
                     /locus_tag="VLE22_06640"
     CDS             699..3143
                     /locus_tag="VLE22_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HSB14118.1"
                     /translation="MNSRMTLCLLLLASLPTCAGGSELPPNRWVEIRRDEDGARRGSG
                     IRYASGAKAFFLWGFHNSNQDLLQEQPLMQVPEYDMVSFDPAAGRWMDHLPRQMKREW
                     SQTLPLAYVPRTYSGITTGSERTVMRCTSDDQEAVPRPDLNIVFDQICYRPRSNALYY
                     FTGGLTAMYDVGKRRWSDLRPAHSPPPVLGGSLEYDPIHDEIVLFGGGHIAERGADGA
                     ARGYTGTWVYSIRDNDWRMLPPAAQPPPRMNTRMVLDSRNQVLVLFGGDGHKSYLADT
                     WLFDIKTRAWRPSRANAGPPPRAGHFTVYDPETGLVIIGGGYNRQDLTDMWGYDAGQD
                     RWQRLAGEVPTGFYLSADIAPERRLILLVTANRKADDQVTCNVLYPVRTTYAYRIGKE
                     APELSEQGAVAHPAMLKRSPEEMKGAHRDPARREEQAKRLAGLPVDRWVLLDRPGREA
                     PTRTWGSATFDTDRGLILYWGGGHCGYEGSDVDAYDVAEHTWRGEVEPEYPERLWNHG
                     VRLAGVTFGGRPWTNHGRKVFAYDPVSRKLIMVEPIRLTTGYEPAWLRAFPTKDSTAP
                     DALVRAPSSYRKFVTWTYDPDTQGWKILGPAPAGLDTFLTTPHGVVGVPVNWPGRLND
                     AGYQLPWRPEDPPEDNAPFLLRESRWEKLSGAGPSPQNLYEMTALVYDSKRDRVLLHG
                     GGQKRDELWSFDLKSRRWSNLRPRVLAPEGGAPPACTREAVYLPGEDVLLIYGSPRTT
                     WVYNPSDNAWRQIDLPVPEGDQRHGSSGQNRAMLYDAKRDIVLLVLGAGGDTGRASVY
                     ALKYRNAEAVVKQKSR"
     gene            complement(3124..4401)
                     /locus_tag="VLE22_06645"
     CDS             complement(3124..4401)
                     /locus_tag="VLE22_06645"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14119.1"
                     /translation="MSIDRRYFLKLAAAGAASLAAAENTVRIGMVGVGGRGTGLLRNL
                     LDLPGVEIPAICDINEDNLRRAQGIVEKAGRPQPAGYSGGPEDFRSLVERKDLDAVVT
                     ATPWEWHTPVCLAAMKADKYAATEVPAAITVEECWDLVNTSEKTGKPCMMLENVCYFR
                     NVLMVLNLIQKGMLGEIIHSEAGYQHDVRGGKIRATDGEIRWRGMHAVKRNANLYPTH
                     PIGPVAWWSNINRGDRFTYLVSMSSKSRGINNYVRKKFGPDHPNAKRTYALGDVNTTL
                     IRTENGVTVTLYHDTQLPRPYDLILRVQGTEGIYSGTLDKIYIEDRSPKPHTWEDIEP
                     YYEKFEHPIWKDLGPTAQNYGHGGGDYITLHQFVKAVRDRTQTPIDVYDTAAWSVITP
                     LSETSVATKSAPVDVPDFTRGKWKTVRPIEISA"
     gene            complement(4425..6134)
                     /locus_tag="VLE22_06650"
     CDS             complement(4425..6134)
                     /locus_tag="VLE22_06650"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSB14120.1"
                     /translation="MLHPLRLLPCMPLMTGLLAGADADLILHNGKILTVDAKFSIVQA
                     VAIKGGRFVAVGDDRAVLAERGSRTKVVNLRGQTVVPGLMDSHVHPLEGGLSEFRESL
                     PVLDSYDAIRNYIREQAKKTPKGQWIIVPRTFPTRLKELRMPTREVLDVATEHPVMLD
                     ASYVVVANTLALKISGITRNTSNPPRGEIVHDEHGEPNGILKNAQSLLKGLKPAVPFT
                     PEEKLRALEKMLSLYRASGLTCIGDGAVGQEEIALFEKLKAQGRLPIRVVMTWWNDAS
                     RPLVALKREVESKPYTTNTGDEWLKFGAYKINFDGGMTIGTAYQRHPYGPFGRQLYGK
                     TNPDDRGQLFQPYDKLLDVLRVAHRKGWALTAHAQGGGAIDLFLDAMEALNREKPIAP
                     TRSHLIHASFQSPEAIARMKKLGILGDVQAAWLYCDAPALEKVFGVEGMRYFFPLRSY
                     IDAGVIVAGGSDHMIGWDRNRAINPYNPFHGMWVSVTRKTVRGSVIQPQERISREEAL
                     KMYTIWAAYRMFSEKARGSIERGKLADLVVIDRDYLTCPDDEITKIEPVMMVLDGKVA
                     FQR"
     gene            6141..7862
                     /locus_tag="VLE22_06655"
     CDS             6141..7862
                     /locus_tag="VLE22_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14121.1"
                     /translation="MILSNSFRSLFLPLAVSVLLSAAEPVTGPLVVSDRWPECTDLVT
                     WTRDVMRLEGLEKASETAQGKAFFRWLRLFNRMATGGMIQAHEGEYGKERYILDSHKH
                     LFIYGWGYCDTTSRIAEAAWVEFKKDRGAAERVCVQHPEGGFHTMYRLRLDGGHGAFD
                     PRYGYYVVERDAADARVLDWDGVGVDGNILRNKTYRHRSAPFFEFPRIEWQRALDLRP
                     AYYESQEAWEKAGSPAENVFGNAMYKMGTRFHDMDFRFPRGTTIERFWDNSARKFYVP
                     AGKSAQREEPFLPSGRFYRVTETMFDGNWVKHDPNYQKCKPYVVTVPRDEGYNQDVSG
                     GRTIGQAWGRITYKPDLRDGIPTDALAPGTTFVHATAAPFLRSASQGGGEAVFDVYSP
                     YVLVDGVLSGELSGAANDGLKVEIRALRSKPQNSGEPDVWSTWDTLYAGPGEFSVPLG
                     KERHNGRQVSIHGVYRFQIRISAQPNAGRRSPAGLNSLKLGLYFENAIMSIPQIFAGR
                     NTIRFKVRNSSELRGPVTVAYRYQTATGERSHERTLRASDFHDNVASYVIEAPGLERC
                     NSLTIQH"
     gene            complement(7803..9083)
                     /locus_tag="VLE22_06660"
     CDS             complement(7803..9083)
                     /locus_tag="VLE22_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HSB14122.1"
                     /translation="MATLLTPGRRVLRVPHVRATNRAAVLQLLRRLKSVSRAEIARRT
                     GLSEAAVSRIVAELMAEDLIVERGGEEATGGRPGIRLQLNESRFRTVGVDIQNWETRV
                     SLGTGTGRVLETVRFRTPSNPNKTLRTIAEQVEALTSNLASGEEPAIGISTRGIVNSE
                     TGIMELGSHPAWVRVAMKDSLTERLRRPVFVENNVRAAAVAEYQYGSLEIQGTRCLLL
                     VKVDEGVGMGIVLDGKLYHGPRMAAGEFGQMVIAYSPGLERHDRPGCVEMLASDTATC
                     ARYRHLTGGKCRSGAGSVTDQVRRVCQLAMEGDPAAREAVTETATYLGVGIANAVWAL
                     DAEAVVINGALTEAWPLVSAAIREQFPTGRQFLNFRSLLLRPSSLGGEASILGALALP
                     FLNVFSSEDLTAGEPSVLDRERITALETRRLNHV"
     gene            9314..12901
                     /locus_tag="VLE22_06665"
     CDS             9314..12901
                     /locus_tag="VLE22_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB14123.1"
                     /translation="MVKKLVLSLALAVPLLAQTGNGVVSGTVLDASRAAIPSAKITLT
                     QTETGVTRNSETSAVGIYYFGSVPIGPYKLDVEAAGFKKWSGTLRVDAGQRVVIDPQM
                     EVGAVEAVVEVTGAAPVITTEGMEIGDIKDAQRIQQLPLNGRLVANLFDLTPGVEGGA
                     NPRVNGMKVGSADMLLDGISFMDRYGGGLRPVQPGLDTIQEFRIETTGSSAQYSRPAT
                     MTLVTKSGTNEIHGSAFWTHRNNFAGLRARQRQDTFTTPPQYIRNEFGLSGGGPLIKN
                     KTFWFAAYEGQRERQARFARAQVPTDNIWGGDFSAMVDADNVPITIYDPLTTAADGTR
                     TPFDGNRIPANRIAPIAATMKSISAAPNIPGNPWLEPTFQTYYPITADITTYTLKGDH
                     VFSERDNLSARYTDSHRPFAQYGGRYGYPIPGSTNAGGTGAQDTTIRSAMARWNHVFS
                     PTLLNEFQASANRAKNHTGTLADSTPWADTLGFPNPFGTTGWPTIYSESPFFYYGGWD
                     ADNPQDQNLTAYQLENNVTWIKSTHTVKFGFKGRQEYNNARELQQSQGSHYFGGDWTA
                     LYDPADQQRVSFTGSGFANMLLGLPTYLSNQYNRGYFYFQQKEIGLYVQDSWKVSQRL
                     TLDLGLRWDKWTVYKEKYNRLVNLDINDYIGKMEVYTPHNTRMEDIPGIPPAVLSSWA
                     ARGLTWKTADEAGFPSGLLPADNNNFAPRIGLAYRMTDKWVLRGGYGIYYWPMPLVQI
                     LASSRTNPPLNLRFQNDLSNLNGRVDNYALSSVPAANDFIGKATVDIEGVAGIDNRAQ
                     AMMPWDIHNWSDNTMQEFNVTFERELMKETALRLSYIGNHGSNLEQRWRWNDQESEYN
                     YQARTGLQASTNPDFRRPNPNWTSGCCNSPIRHNGFSNSHSLQAEVERRYSNGLAYQA
                     FYTFSRVQTTTDTGGFNFGSSGINSSGSSSAFAVPENRLILGNPNLSDDERLRMGYAN
                     SVEVPAHRIRFNGIYDLPFGKGQRYGNGASGLVNHLIGGWQIAFIGNWRSGNWMGVDA
                     SRYLFGDPTLDSGQRLEMTYNGRQQRLWFRGYLDPSLASGVDQSALQALVPLDRSQRV
                     YRPVGANFDNRVQMQLADGSVRNTSISENLSWNARNFFRGAGAWNTDLSLFKNIYFTE
                     RIKTRLTADFFNAFNHPLDLNPATATGLQDLSRQANDPRIIQFSLRVEF"
     gene            13358..15709
                     /locus_tag="VLE22_06670"
     CDS             13358..15709
                     /locus_tag="VLE22_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB14124.1"
                     /translation="MSALRQSSVFGAVLSVGVLLLGASCRTQQPAKQARSSDRIYRFD
                     DQLETAASVSAANVSTAARVTDPVVWKNFYTEKDATWELLRGNMGLRDGDLIVKGDGG
                     SPVIVSPKAFPIDWSLFESVQIRMMAEGGSEVKIKIGDLEMKRKLAPPKQYQVYRFDV
                     NLDVPRGSRPLAIMPTDSPIHLAAIDFIELVPRRTSFPKAVGRHFTGKRDEYRNTIYA
                     HSPSKIAYEVRVPEKGLLSFGVGVAEKGKSVTFRVLADGSSTPLYSKSLDDPDIWVDA
                     SVDLSAFAGRTTKLVFETGSESRGAVGMWANPLLTVRAPKARPNVLVYLVCSLRPDHT
                     SLYGYTRETTPFLKRLGASSVVFDDAQAQAPWTKASVPSLLTSLYAYTHGIARDTDAI
                     PQGATTMAETLRAAGYVTASVVTNPFAGRVSGLEKGVDYLMEYAVVHRQRTDEVDRGT
                     DSAALNKVIIPWLEQHRNEPFFLYAHATDPHAPYRPPAGFEEKFANPAESVAFDRDYD
                     RLRDKLQYGGGATISRANSKAKGIDPDSFLRRAIDRYDGEILHNDHSLEVLVNKLKEL
                     GLLDNTLLVVLSDHGEEFWDHGWTAHGHSLYQELTHTVLLMSNSKLLPTPRRVPDTVQ
                     LIDLMPTLIEMLGIQPKGIIQGQSLVPLLKGRPLRRKVPVMSSKPRHPLAKPTGFVPE
                     NRTGTFAIWDGPWKLLYRDQARLAGLPEVELYERRGDRGETTNVAVQNPDVVKKLRAE
                     IDRWMKAQDQIKKLLGPGGTTTLDKQSIERLRSLGYLGGKSSQ"
     gene            15735..>16822
                     /locus_tag="VLE22_06675"
     CDS             15735..>16822
                     /locus_tag="VLE22_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HSB14125.1"
                     /translation="MPAYRTLGLLLCLFSILSAETSTSLWSRGYSIVPSPQRVQLDGQ
                     DVVLGTSWTVDAGPLGPRHIAARTLLRDFESLHGLTLKTGGRDASIRLSIAPGTVITG
                     GDEEIDRQGYRLTIRPDRIEIMGNAEEGLFYGVQSLLQLARRSADGRLTVPRVVIEDW
                     PSLQLRFLHWDTKHHQDRMETLKRYLDWAARLKVNMIGFELEDKFEYPAHPVIGAPGA
                     FTTAELQELVDYGLERYIQVVPEIQSPAHMAYVLKHPEFAGLRSNGNNYMANLCDERS
                     YDLIFSMYDDVIKATKGVGYFFVSTDEIYYAGDDPGCKEPYNPVNRSLKWAEFAKRAR
                     DFLASRNRRMLAWVEYPLLPEHVKLLPP"
BASE COUNT         3344 a         5114 c         5075 g         3289 t
ORIGIN      
        1 gggcagcagc ttcgatacta tccgtccgtt cgggagacca tccggaaaat tcacgaagag
       61 aaagttgctg gccagacgtt tgtggtcaag gcgcaacggg atagtgcgcc gagccgaaaa
      121 ggaccgtcca accgcccgtc ctggtatgac gatgtcaaac gctccggaga tctcatcgtc
      181 gagaacgccg tgcacaacat cgatgcctgc aactggatcg tgggaagccg ccccgtcagt
      241 gcatacggtc acgggaagaa atatctgccg cagccgatcc cggccggaac catcatgatg
      301 gacggtttca gcgtcgagta catttacgag aacgacgttc acgtcgacta tagccaactc
      361 gccttccatg cacgggggct gaaggaactg ccgaacggca tgtggtacac ggtgttcggc
      421 gacaagggta gcgtgttcat gacgcacgag tcggctgtgc tgtacgacct ttatggcgag
      481 tcggaagcaa aagatctatt gacgaccgaa ctcaaggagg ggaaagagga cgccgtcgcc
      541 cagttcttcg cctgcatccg cgagaagcgc caacccttcg ctgacgtcca ggtggcagcc
      601 attgcggcct tgacagccat catgggccgt gaggcgatct accggcggcg tatggttacg
      661 tggaaggaac ttggcgtcga agtctgacgg aggctcacat gaattcgcgc atgacgctct
      721 gtttgttgct tctcgcctcc ctgccgacgt gcgctggcgg aagcgaactg ccacccaacc
      781 gctgggtgga gatccggcga gacgaagacg gcgcccggcg aggttccggc attcgatacg
      841 cgtctggcgc aaaggcattc ttcctatggg gtttccataa ctccaatcag gatcttctgc
      901 aagagcagcc gttgatgcag gtacccgagt atgacatggt gtccttcgat ccggcggcgg
      961 gtaggtggat ggatcacctt ccgcggcaga tgaagaggga gtggagccag actctgcccc
     1021 ttgcctacgt gccgcgaaca tattcgggga tcaccaccgg cagtgaacga acggtgatgc
     1081 ggtgcacctc ggacgatcag gaggcggtcc cgcgtcccga cctgaacatc gttttcgacc
     1141 agatttgcta taggccgcgc agcaacgcgt tgtactactt cactggcggg ctgactgcga
     1201 tgtacgacgt cgggaagagg cgctggtcgg acttgcgccc agcgcactcg ccgccaccgg
     1261 tcctcggcgg ctcgctcgaa tacgatccga tccacgacga gattgttctc ttcggaggcg
     1321 gccatatcgc ggagaggggt gcagacggtg cagcgcgtgg gtacaccggc acatgggtct
     1381 acagtatccg cgacaacgac tggcggatgc tgccgccggc ggctcaaccc ccaccgcgaa
     1441 tgaatacgcg gatggtcctc gacagccgca accaggttct cgtgctgttc ggaggcgatg
     1501 ggcacaagtc gtacttggcc gacacatggc tgttcgacat caagacgcgg gcgtggcggc
     1561 catcgcgtgc gaacgccgga ccacccccgc gtgccggcca cttcaccgtg tacgatccgg
     1621 aaacgggtct ggtgatcatc ggtggcggat acaaccggca ggatctgacc gatatgtggg
     1681 gttatgacgc cggccaggac cggtggcagc ggcttgccgg ggaggtccca accggcttct
     1741 acctgagcgc cgacatcgct ccggaacgcc gcctgatcct gttagtgaca gccaaccgga
     1801 aagcggacga ccaggtaacg tgtaatgttc tgtatccggt gcgtacgacg tacgcgtatc
     1861 ggatcgggaa agaagcgccc gaactttcag agcaaggcgc cgtagcgcac cccgcgatgc
     1921 tgaaacggtc gccggaagag atgaaaggcg cacaccggga tccggcgcgt cgcgaagaac
     1981 aggcgaaacg tttggcagga ttgccggtag accgctgggt gcttctcgac cggcccggaa
     2041 gggaggcgcc gacccgcacc tggggcagcg ccacattcga tacggaccgc ggcctgattc
     2101 tttactgggg cggcggccac tgcggctatg aagggagcga cgtggacgcc tatgacgtcg
     2161 ccgaacacac ttggcgcggc gaggtggaac ctgagtaccc ggagcgcctg tggaaccacg
     2221 gcgtgcgact ggctggtgtc acgttcggcg gcaggccgtg gaccaaccac ggacggaaag
     2281 tcttcgccta tgatccggtg agccggaagt tgatcatggt cgaaccgatc cgtctgacca
     2341 cgggatacga gcccgcttgg cttcgcgcct tcccaacgaa ggattcgacc gcgccggacg
     2401 ctctcgtcag agcaccatcg tcgtaccgca agtttgttac gtggacctac gatccggata
     2461 cgcaaggatg gaagattctc ggtccggctc ccgcgggact ggatacattc ctgacaacgc
     2521 ctcatggtgt ggttggggtc ccagtgaact ggccggggcg tctgaacgat gctggctacc
     2581 aactcccttg gcgtcctgaa gacccgccgg aggataacgc gccgtttctt ctgcgcgaat
     2641 cgcgctggga gaagctcagc ggagccgggc cttcgcctca gaacctgtac gagatgacag
     2701 ccctcgttta cgactcgaag cgcgaccggg tactcctgca tggaggcggc caaaagcgcg
     2761 acgaactgtg gagtttcgat ctgaaaagcc gccgctggag caaccttcgg ccgagagtac
     2821 ttgcgccgga aggaggggcg ccgccggcgt gcacgcgcga agcggtgtat ctgcccggcg
     2881 aggatgttct tctcatttac ggaagcccgc gcacaacttg ggtgtacaac ccttcggaca
     2941 acgcttggcg gcagatcgac ctgccggtgc cggaaggcga ccagcggcac ggaagctcag
     3001 gacaaaatag ggctatgctc tatgatgcga aacgcgacat tgtactgctc gtcctcggcg
     3061 ccggtggaga cacaggccga gcgagcgttt acgcgctgaa ataccggaac gcggaggcgg
     3121 tggtcaagca gaaatctcga taggccgtac cgttttccat ttcccgcggg tgaagtccgg
     3181 aacgtcgaca ggtgcgctct tcgtggcgac cgaagtctcg ctgagcggcg taatgacgct
     3241 ccacgccgcg gtgtcataga catcgatcgg cgtctgggtt cggtcccgga ctgctttcac
     3301 gaactggtgc agggtgatat agtcgccgcc tccgtgtccg taattctgtg cagtgggccc
     3361 aaggtccttc cagatcgggt gctcgaactt ctcgtagtaa ggctcgatgt cctcccaggt
     3421 gtgaggcttc gggctcctgt cttcgatata gatcttgtcg agcgtcccgg aataaatgcc
     3481 ctcagttccc tgcacgcgga gaatgaggtc atagggccgc ggaagctgtg tgtcgtggta
     3541 gagggtgacc gtcacgccgt tctcggttcg aatcagggtt gtgttaacgt cgccgagcgc
     3601 gtacgtccgc ttcgcattcg gatggtcggg accgaacttt ttcctgacgt agttgttaat
     3661 gcctcgcgac ttgctgctca tcgagacgag ataggtaaag cgatcgccgc ggttgatgtt
     3721 cgaccaccac gccaccgggc cgatgggatg ggtcgggtaa aggttggcgt tccgcttcac
     3781 cgcgtgcatg ccacgccagc ggatttcgcc gtcggtggca cggatcttgc cgccgcgcac
     3841 gtcgtgctgg taaccggcct cagagtgaat gatttcgccg agcattccct tctggatgag
     3901 gttgagcacc atgagcacgt tgcggaaata gcacacgttc tcgagcatca tgcagggctt
     3961 cccggtcttt tctgacgtat tgaccagatc ccagcactcc tccacggtga tggctgcggg
     4021 cacctctgtg gcggcgtact tgtcggcctt catcgcggcc aggcacaccg gggtgtgcca
     4081 ttcccagggc gtggcggtca cgacggcatc gaggtcttta cgctccacca ggcttcggaa
     4141 atcctcgggg ccgccggaat atccagccgg ctggggcctt ccagcctttt cgacgatacc
     4201 ttgcgcgcgc cggagattgt cctcgttgat atcgcagatt gccgggatct ccacgccggg
     4261 gagatcgaga aggttgcgca ggaggccggt accccgccct ccgacaccca ccatgccgat
     4321 ccggacggtg ttttcggcgg cagccagcga cgcggcgccg gcggctgcca gtttcaggaa
     4381 gtatcttcga tcaatgctca ttcccatcgc tggttctcca agaactaccg ttgaaaggcg
     4441 acttttccgt cgagcaccat catgaccggc tcgatcttcg tgatctcgtc atcgggacag
     4501 gtcagatagt cgcgatcaat caccaccagg tcggccagtt tcccgcgctc gatcgagccg
     4561 cgagccttct ctgaaaacat ccgataggcc gcccagatcg tgtacatctt cagcgcttct
     4621 tcccggctga tgcgctcctg cggctgaatc acgcttcccc ggaccgtttt tctagtcacc
     4681 gagacccaca tgccgtggaa cgggttgtac gggttgatcg cgcgattcct gtcccacccg
     4741 atcatgtgat cacttccgcc ggcgacgatc actccggcgt caatgtacga gcgcagtgga
     4801 aagaagtatc tcatgccctc aacgccgaat accttctcca gcgcgggcgc gtcgcaatag
     4861 agccaggcgg cctgcacgtc gccgaggatg ccgagtttct tcatgcgcgc aatcgcttcc
     4921 ggactttgga agctcgcgtg aatcaggtgt gagcgggtcg gagcgatcgg tttttcccgg
     4981 ttaagcgcct ccatggcgtc gaggaacaag tcgattgcgc caccgccctg cgcgtgggca
     5041 gtcagcgccc aacctttccg atgcgccacc ctgaggacgt ccagcaactt gtcgtagggc
     5101 tggaacaatt gcccacgatc gtcggggttc gttttcccgt aaagttgcct cccgaacggg
     5161 ccgtacggat gccgctgata ggcagtgccg atggtcatgc ccccgtcgaa gttgattttg
     5221 taagccccga acttcagcca ctcgtcgccg gtgttggtcg tgtacggctt cgactccacc
     5281 tcccgcttca gagccacgag cgggcgcgac gcgtcgttcc accatgtcat cacgacgcgg
     5341 atcggcagcc gcccctgcgc cttgagtttc tcgaaaagcg caatctcttc ttggccgaca
     5401 gcaccgtccc caatgcacgt cagccccgac gcccggtaaa gggaaagcat cttttcgagc
     5461 gcccggagtt tctcctccgg cgtgaacggc accgccggct tcagtccctt gagcaacgac
     5521 tgggcgttct tcagtatccc gttcggctcg ccgtgttcgt cgtgcacgat ctcgccgcgc
     5581 ggtgggttgg aagtgttccg cgtgatgccg cttatcttga gcgccagcgt gttggcgacc
     5641 accacgtagc tggcatccag catcacggga tgctcggtcg ctacgtcgag cacctcgcgg
     5701 gtcggcattc gcagttcctt caacctcgtc gggaacgtgc ggggaacgat gatccactgc
     5761 cctttcggcg tcttctttgc ctgttcgcgg atgtagttcc ggatcgcgtc gtacgaatcg
     5821 agcacgggca gcgattcccg gaattcgctg agccctcctt cgagcgggtg aacgtgactg
     5881 tccatcaggc ccggaaccac ggtttggccg cgaaggttga cgactttggt tcttgaccca
     5941 cgctcggcta ggaccgccct gtcatctccc actgcaacga agcggccgcc tttgatcgcg
     6001 accgcctgaa cgatcgagaa cttcgcgtcc acggtgagga tcttaccgtt gtgaagaatc
     6061 aggtcggcat ccgcgccggc gagcaggccc gtcatcagcg gcatgcaggg caggaggcga
     6121 agggggtgta acatgacact gtgattctat cgaattcatt ccgcagtcta tttctgcccc
     6181 ttgccgtatc cgtcttgctg agcgccgctg aaccggtcac cgggccgctg gtggtcagcg
     6241 accgttggcc cgaatgcacg gacctggtga cctggacacg tgacgtcatg cggttggagg
     6301 gccttgagaa agcctccgaa acggcgcaag ggaaggcctt cttccggtgg cttcgcctat
     6361 tcaaccgaat ggctacgggc gggatgattc aggcgcacga gggcgaatac gggaaggagc
     6421 gttacatact cgactcacac aaacacctct ttatctacgg gtgggggtac tgcgacacga
     6481 cgagccggat cgccgaagcg gcctgggtgg agttcaagaa ggaccggggt gcggccgagc
     6541 gcgtgtgtgt gcagcacccc gagggcggct tccacacgat gtaccggctg cgtctcgatg
     6601 gaggccacgg cgccttcgat ccacgatatg ggtactacgt cgtggaacgt gacgcagcgg
     6661 acgcgagggt gctcgactgg gacggcgtcg gcgtggacgg aaacatcctg agaaacaaga
     6721 cctatcggca ccgaagcgcg ccgtttttcg aattcccgag gattgagtgg caacgcgcgc
     6781 tggatcttcg cccggcgtac tacgaatcac aggaagcgtg ggaaaaggcc gggagccccg
     6841 cagaaaacgt cttcggcaac gcaatgtaca agatgggcac ccggtttcac gacatggact
     6901 tccggtttcc gagggggacg accatcgagc gcttctggga caattccgcc cggaagtttt
     6961 acgtgccggc gggaaagagc gcgcagcgcg aggaaccgtt cctgccttcc ggccgcttct
     7021 atcgggtgac cgagacgatg ttcgacggaa actgggtgaa gcacgacccc aattaccaga
     7081 agtgcaagcc ctacgtcgtt accgtaccaa gggacgaggg ctacaaccag gatgtgagcg
     7141 gcgggcgcac aatcggccag gcctgggggc ggatcacgta taaacccgat ctgcgcgatg
     7201 ggatcccaac ggatgcattg gcccctggaa ccacgtttgt tcacgccacg gcggcaccgt
     7261 tcctccggtc ggcttcgcaa ggcggtggag aagcggtctt cgacgtgtac agcccctatg
     7321 tcctcgtcga cggagttctc tcgggcgaac tctccggggc agccaatgac gggctgaagg
     7381 tcgaaatccg cgccttacgc agcaaaccgc aaaactccgg tgagccggac gtttggagca
     7441 cctgggacac actctacgcg gggcccggcg agtttagtgt cccgctcggc aaggagcgac
     7501 acaacgggcg ccaagtcagc attcatggcg tctaccggtt ccagatcaga atctccgcgc
     7561 aaccgaacgc cggccgtcgc tcgccggccg gactgaattc cctgaaattg gggctgtact
     7621 tcgagaacgc gatcatgtca atcccccaga tcttcgccgg acggaacact atccgcttca
     7681 aagtgcggaa ttcgtctgag ttgcggggac cggtgacggt cgcctatcga taccagactg
     7741 cgacgggtga gcgtagccac gagagaacac ttcgagcctc agattttcac gataatgttg
     7801 cctcatacgt gattgaggcg cctggtctcg agcgctgtaa ttcgctcacg atccagcact
     7861 gagggttcac cggcggtcag atcttcggag gaaaacacgt tcaggaacgg cagagcgagc
     7921 gctcccagga tcgaagcctc gccccccagc gaggacggac gcagcagcag gctccggaag
     7981 ttcaggaatt gacgaccggt cgggaactgc tcccggattg ccgccgaaac cagcggccat
     8041 gcctccgtca gggcgccgtt gatcaccacc gcctccgcgt ccagtgccca gactgcattc
     8101 gcgatgccga cgcccaagta agtggccgtc tcggtcaccg cctcgcgtgc cgctggatcc
     8161 ccctccatgg ccagttggca aacgcgtctc acctggtctg tgacgctccc ggcgccggac
     8221 ctgcattttc ccccggtgag atggcggtag cgtgcgcagg tggcagtgtc cgacgccaac
     8281 atctccacgc agcctggccg gtcatgccgc tccagtccgg gagaatacgc gatcaccatc
     8341 tgcccgaact caccagcggc catacgcggg ccgtgataga gcttaccgtc gagaacgatg
     8401 cccatgccga cgccttcgtc caccttcact aggagcaggc accgggtgcc ttggatctcg
     8461 agacttccgt actgatactc cgcaacggcg gccgcgcgca cgttgttttc cacgaatacg
     8521 ggccgccgca gcctctccgt cagagagtcc ttcatcgcga cacgcaccca ggccgggtgg
     8581 ctgcccagtt ccatgatgcc cgtctcgctg ttcacgatgc cacgggtgct gatgccaatg
     8641 gctggttcct caccgctcgc aagattcgac gtgagagcct cgacctgttc tgcaatcgtc
     8701 cgcagagtct tgttgggatt ggagggagtg cggaaccgga ctgtctccag caccctgccc
     8761 gtgccggtgc ccaacgagac tcgcgtctcc cagttctgaa tgtcgacacc cacggtgcgg
     8821 aaccgcgatt cgttgagttg aagacggatg cccggtcttc cgccggtcgc ttcctcccca
     8881 ccccgctcta cgatgaggtc ctccgccatt aactccgcca cgattcgcga aaccgctgct
     8941 tcggacaggc ctgtccgccg cgcaatttcg gcgcgggaca cgctttttaa ccggcggaga
     9001 agctgaagga ccgcggcccg gttcgtggcc cgaacatgcg gtacccgcaa gacacgtcgc
     9061 ccgggcgtta agagcgtagc catgactgtc acttcgttgc ttccggcaac gagcagcagt
     9121 tcattgtaga gaaacggaga gatttgagca agaccttaat tttctccgag gcgtcgtccg
     9181 ctcaggccgc caaagcggac agacgccgga cggatcacat gttaacgaca accccctacc
     9241 cccttgacac gtcctccgat tcaagatacc cttagccaga atcgcttcta tattttgggg
     9301 gtttaactta acaatggtaa aaaagcttgt gttgagcctc gctttggctg tgcctttact
     9361 ggcgcagacc ggcaacggcg tcgtttcggg caccgtgctc gacgcatcga gggcagccat
     9421 cccgtccgcc aagatcacat tgacccaaac ggagacgggg gtgacgcgaa actcggaaac
     9481 gtcagccgtc ggcatctatt atttcggctc ggtaccgatc gggccataca aattggacgt
     9541 tgaagcagcc ggattcaaga aatggtccgg cacgcttcga gtagatgcgg gtcagcgcgt
     9601 agtgattgat ccgcagatgg aagtcggcgc agttgaagcc gtcgtggagg tcaccggcgc
     9661 ggcgccggtc atcacgactg agggcatgga aatcggcgac atcaaggatg cgcagcgcat
     9721 ccagcagttg cctctcaacg ggcggctggt cgccaacctg tttgacctga cgccaggcgt
     9781 cgagggcggc gccaatccgc gcgtcaacgg gatgaaggtc ggctccgccg acatgctgct
     9841 cgacggcatc tcttttatgg atcgatacgg cggtggcctg cgtcctgttc agcccgggct
     9901 ggacacgatt caggagtttc gtatcgagac aaccggctcg agcgcgcaat attcccgtcc
     9961 ggcgacgatg acgctagtaa ccaaaagcgg caccaacgag atccacggca gcgcattttg
    10021 gacgcaccgc aacaattttg ccggcctgcg agcgcggcag cgacaggaca cctttacgac
    10081 accgcctcaa tatatccgca acgaattcgg actctccgga ggcggcccgc tcattaagaa
    10141 caagaccttc tggttcgccg cttatgaagg acagagagag cggcaggcta gattcgcccg
    10201 cgctcaggtt cccacggata acatctgggg cggcgatttc agcgcaatgg ttgatgccga
    10261 caacgtgccg atcaccattt acgatccgct gacgactgcc gctgatggaa cgcgaactcc
    10321 gtttgacggc aaccggatac cagcgaatcg catcgcgcca atcgcggcta cgatgaagag
    10381 catctcggcc gcgcccaaca tacccggcaa cccctggctg gagccaacct tccagacgta
    10441 ctatcccatc accgccgaca tcacaacgta taccctcaaa ggcgaccatg tgttttctga
    10501 gcgcgacaat ctctcggcca ggtacacgga ctcgcaccgg cccttcgcgc aatacggcgg
    10561 ccggtacgga tatccgattc cgggatcgac caacgccggc ggcaccggcg cccaggacac
    10621 gaccatccgt tcggctatgg cgcggtggaa ccacgtgttt tcgccgacat tgttgaacga
    10681 atttcaggct tcggccaatc gcgcgaagaa ccacacgggc acgctggctg acagcactcc
    10741 gtgggcggat acgctcggct tcccgaaccc cttcggtacc acaggctggc ccaccatcta
    10801 ctcggagagc cccttcttct actatggcgg ttgggatgcc gataacccgc aggatcagaa
    10861 cctgacggcg taccagctcg aaaacaacgt tacctggatc aagagtaccc acaccgtgaa
    10921 gtttggcttt aagggccggc aggaatacaa taacgcgcgc gagttgcagc agtcccaggg
    10981 atcgcattat ttcggtggcg actggaccgc cctgtatgat cccgccgacc agcagcgcgt
    11041 ctctttcact ggttccggct tcgccaatat gctgctgggg cttcccacgt atctttccaa
    11101 ccagtacaac cgcggctact tctacttcca gcagaaggaa attggcttgt atgtgcagga
    11161 cagctggaag gtgagtcagc gcctgacgct tgatctcggt ctccggtggg acaagtggac
    11221 agtttacaag gaaaagtata accgtctggt aaacctcgac atcaacgact acatcggcaa
    11281 gatggaagtc tacacgccgc acaacacccg gatggaagac atcccgggca tcccgccggc
    11341 tgtactcagc tcatgggcgg cgcgcggtct gacctggaaa acagccgacg aggccggctt
    11401 cccgagcggc ttgctgccgg cggacaataa caacttcgcc ccccgtattg ggttggcata
    11461 ccgcatgacc gataaatggg tgctccgcgg cggctacggc atttactact ggccgatgcc
    11521 gctggtgcag atcttggcga gttcgagaac gaacccgccg ttgaacctcc gcttccagaa
    11581 tgacttgagc aacctcaacg gcagggtgga caactatgcc ttgtcgagcg ttccggcggc
    11641 gaacgacttt atcgggaagg ccactgtgga catagagggt gtagcgggaa tcgacaaccg
    11701 cgcgcaagcg atgatgccct gggacatcca caactggagc gacaacacga tgcaggagtt
    11761 caacgtcacc ttcgagcgcg aattgatgaa ggagacggct ctgcggttga gctatatagg
    11821 caaccacggc agcaatctgg agcagcgctg gcgctggaac gaccaggaat ccgagtacaa
    11881 ctaccaggcc cgcaccggcc ttcaggcgtc aaccaatccc gactttcggc gcccaaatcc
    11941 gaactggacc agcggttgct gcaactcgcc catccgacac aacggcttct cgaactctca
    12001 ctccttgcag gctgaggtgg agcggcggta ttcgaacggg ctggcttacc aagcgtttta
    12061 cacgttctcc cgcgtgcaga ccacgacgga taccggcgga ttcaacttcg gttcgagcgg
    12121 catcaactcg agcggcagca gcagcgcctt cgccgttccg gagaatcgtt tgattctggg
    12181 caatccgaac ctttccgatg atgagcggct tcgtatggga tatgccaatt cggtggaagt
    12241 gccggcgcat cggatccggt tcaacggcat ttacgacctg ccgtttggca agggccagag
    12301 gtacggcaac ggcgcgagcg gcctggtgaa tcacctgatt ggcggatggc agattgcttt
    12361 catcggcaac tggcgctccg gcaattggat gggggtggac gccagccgat acctgtttgg
    12421 tgatcccacg ctggattccg gccagcggct ggagatgacc tacaacggtc ggcagcagcg
    12481 tctatggttc cgcggctatc tcgaccccag tttggcgtca ggcgtggatc agtcggcgct
    12541 tcaggcgctg gtcccgctcg accgctcgca acgtgtctac cggcccgtcg gagccaactt
    12601 cgataaccgc gtgcagatgc agcttgctga cggcagcgtc cgcaatacct cgatctcgga
    12661 gaacctgagc tggaatgcgc ggaatttctt tcgtggagca ggcgcctgga acacggacct
    12721 gtctctgttc aagaacatct acttcaccga gagaattaag actcgtctca cggccgattt
    12781 cttcaatgcg ttcaatcatc cgttggacct gaatccggcc acggccacag ggctgcaaga
    12841 cctgagccgg caggcgaacg atcctcgcat cattcagttc tccttgcgcg tcgagttcta
    12901 accagattgc gcaggtgact ttcgggcgga gcgcggtttt tcggaccgca ctccgccttt
    12961 ttcgttttgc ggcccgaaca tacttttgat ctcggccccc atgcgagggc gcccctctgc
    13021 tttctgggcg gttctgtccg tcggcctgtt ccttctggga ggatgcgccg ggggatcaca
    13081 cttggagggg tgagttcatt acctccggca attaaccagc caagcacagc aagtctgact
    13141 ctcgtcagat tccgccggct tgagggctcg atactttgcc tcttgacatt cttgaccgtt
    13201 ttacagtagt ccgagccttc gagtagtgag gttcgcttag ggagataatc atgacggctt
    13261 tcttgcctta tcttaggaat tcctcgtgtc tcggtttttc ggaccccact ccgccttttt
    13321 cgcccagcgg cccggatata cttgtgattt cagccccatg agtgcactcc ggcaatcctc
    13381 agtttttgga gcggttctgt cggtaggcgt actgcttctg ggagcttcct gtcgcacaca
    13441 acagccggcg aagcaggcgc ggtcctcgga tcgcatctat cggttcgacg atcaacttga
    13501 aacggcggcg tccgtctcgg cagcgaatgt ctcgaccgct gcgagggtga ctgacccggt
    13561 tgtgtggaag aacttctaca ccgagaaaga cgccacctgg gaactgcttc gaggtaacat
    13621 gggcctccgt gacggcgatc tcatcgtgaa gggggatggc ggatcgcccg tgatcgtctc
    13681 gccgaaggcg tttccgatcg actggagcct gttcgagagc gttcagatcc ggatgatggc
    13741 cgagggcggt tcggaagtca agatcaagat cggcgacctg gagatgaaac ggaaactggc
    13801 cccgccgaag cagtatcagg tctaccgttt tgacgtgaat ctcgacgtgc cccgtggcag
    13861 ccggccgctc gcgatcatgc cgacggacag cccgattcac ctcgcggcca tcgacttcat
    13921 cgaactcgtg ccgcgcagga cgagttttcc gaaggcggtg ggccgccatt tcaccggaaa
    13981 acgcgacgaa taccgaaata cgatctacgc ccattcccca tccaagattg cctacgaagt
    14041 gcgcgtgccc gagaaagggc tgctgagttt cggagtcgga gtcgcggaga agggcaagtc
    14101 ggtcaccttt cgtgtgctcg cggatgggtc gagcacgcct ctctactcga aatcactgga
    14161 cgatccagat atctgggtgg acgccagtgt agacctgtcc gcgttcgccg gcaggaccac
    14221 caagctggtc tttgaaactg gttccgagtc gcggggcgct gtcggcatgt gggccaatcc
    14281 gctgctcact gtccgagcgc cgaaagcgcg gccgaatgtt ttggtctacc tcgtgtgcag
    14341 cctcaggccg gaccacacta gcctgtacgg ttacacgagg gaaaccacgc cgttcctcaa
    14401 acggctcggc gcatccagcg tggtcttcga tgacgcgcag gcacaggccc cgtggacgaa
    14461 ggcctcggtg ccgtcgctgc tgacctcgct ctacgcgtac acgcacggca tcgctcgcga
    14521 cacagacgca attccgcagg gcgccaccac gatggccgag accctgcgtg ccgccggata
    14581 cgtcacggcc agtgtcgtga ccaatccgtt tgccgggcgg gtatccgggc tcgagaaggg
    14641 tgtggattac ctgatggagt acgcggtcgt tcaccgccag cgaacggacg aagttgaccg
    14701 tgggaccgac tccgcggcct tgaataaggt gatcattcct tggctggaac agcatcggaa
    14761 cgagcctttc ttcctctatg cccatgcgac cgacccacac gctccctatc gtcctccagc
    14821 cggcttcgag gagaagttcg caaatcccgc ggagagcgtc gccttcgacc gtgactacga
    14881 ccggcttcgc gacaaactgc agtacggcgg tggggccacc atcagtcgag ccaacagcaa
    14941 ggcgaagggt atcgaccctg attccttctt gcgccgtgcc attgaccgtt acgacggaga
    15001 gattctccac aatgaccaca gcctcgaagt cctggtcaac aaactgaagg aactcggcct
    15061 gctcgataac accctgctcg tcgtcctttc ggatcacggc gaggaattct gggatcatgg
    15121 ctggaccgcc catggccact cgctctatca ggaactgaca cacaccgtct tactgatgtc
    15181 gaactcgaag ctgttgccta ccccacggcg cgtgccggac accgtccagt tgatcgacct
    15241 gatgccgacg ctgatcgaga tgctcggaat ccaacccaag ggcatcatcc agggccagag
    15301 tctcgtgcca ctactgaaag gccggccgct gaggcgcaag gttccggtga tgtcctccaa
    15361 gccgcggcat cccctagcca agccgaccgg cttcgttccg gagaatcgga cgggcacgtt
    15421 cgccatctgg gacgggccgt ggaaattgct gtaccgggac caagcccggc tggcgggttt
    15481 gcccgaagtg gaactgtatg aacgccgcgg tgatcgcggg gaaacgacga acgtggcggt
    15541 acagaatcca gatgtcgtca agaagttgag ggccgagatc gaccgatgga tgaaagccca
    15601 ggaccagatc aagaaactgc ttgggccggg aggaacgacg accctcgaca aacagtcgat
    15661 cgaacgtctg cgcagccttg gatacctcgg cggcaaatcg tctcagtaat ttcggacagg
    15721 aattggacac acgtatgccg gcataccgca cactcggact cctgctttgc ttgtttagta
    15781 ttctttccgc ggagacctcg acctcattgt ggtcgcgcgg gtactcgatc gttccgtcgc
    15841 cacaacgtgt tcagctcgac ggccaagacg ttgtgctggg cacctcatgg accgtagatg
    15901 ccgggcctct cggcccgcga catatcgcgg ctcgcacatt gcttcgggac ttcgagtctc
    15961 tgcacgggtt gacgctgaaa actggtggaa gagacgcctc gattcggctg tccatcgctc
    16021 ccggaaccgt cattaccggc ggcgacgagg agattgaccg gcagggctat cggctgacca
    16081 tccgcccgga ccggatcgag atcatgggca atgccgaaga aggcctgttc tacggcgttc
    16141 aatctctgct gcaacttgcg agacggtcgg ccgatggtag gttgaccgta ccgcgcgtgg
    16201 tgatcgaaga ttggccaagt ctccaattgc gattcctgca ctgggacacc aagcaccacc
    16261 aggaccgcat ggaaacgctg aagcggtatc tcgactgggc cgcccgtctc aaggtgaaca
    16321 tgatcggctt cgagcttgaa gacaagttcg agtatccggc gcatcccgtg atcggtgctc
    16381 cgggagcgtt cactactgcg gagttgcagg aactcgtcga ctacgggctg gaacggtata
    16441 tccaggtcgt gccggagatt cagtccccgg cgcacatggc ctacgtgctg aagcatccgg
    16501 agttcgccgg cctgcgatcc aacgggaaca actacatggc gaacctgtgc gatgagcgtt
    16561 cgtacgacct gatcttctcg atgtacgacg acgttatcaa ggcgacgaaa ggcgtgggct
    16621 atttcttcgt ttccaccgac gaaatctact acgctggcga cgacccaggg tgtaaggagc
    16681 cctataatcc agtcaatcgc agtcttaagt gggcggagtt cgccaagcgc gcccgcgatt
    16741 ttcttgcgtc gcgcaatcgc aggatgcttg catgggtgga atatcctctc ctgcccgaac
    16801 acgtgaagct gctgcctccg ga
//
LOCUS       DATIFQ010000144         3370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1772991, whole genome shotgun sequence.
ACCESSION   DATIFQ010000144 DATIFQ010000000
VERSION     DATIFQ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772991"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1524)
                     /locus_tag="VLE22_06680"
     CDS             complement(187..1524)
                     /locus_tag="VLE22_06680"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HSB14126.1"
                     /translation="MAWILILFLFWQSPAVAQTTQRKKAPVVGQVPGPAKKSAPAKQV
                     APTDTRWTLESLSVEGNSTYSRKQIVAATGLRIGQLAGKREFDAARDRLMATGVFESV
                     AYRFGPAANGKGYAATFQVLEVAQVYPIRFDRLDAPPDQLTQVLKKSDPFFGDNIPAT
                     DVVLKRYAKVIEDYLASQGKKETVIGRLTVDEPNRIVILFRPEAAPPAVAEVTFTGNT
                     VLPLTALQNAIAGVAVGAPFSESRFRQILDTTIRPMYEERGRIRVAFPSVRTAKARQV
                     NGLAVTVEVVEGESYKLGEVGFAAAGLPEKDLRKAVGLKSGEIANFTEVKAGLDRIRK
                     FLGRNGYMSPELRTDRTIHDDAKTVDVIIHVEQGQRFTFGKLTIKGLDIHGEAAIRQL
                     WALKPGAPYNIEYPDYFLARIVEDNVFDNLKKTKAIPDINESDKTVDVTLVFN"
     gene            complement(1606..1776)
                     /locus_tag="VLE22_06685"
     CDS             complement(1606..1776)
                     /locus_tag="VLE22_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14127.1"
                     /translation="MRPRLLALWYICIGAGFFTLGVRSVMLGAPWWTVILRWVIAAGF
                     VVIGVVELRRRR"
     gene            1835..2446
                     /locus_tag="VLE22_06690"
     CDS             1835..2446
                     /locus_tag="VLE22_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14128.1"
                     /translation="MRRDGFNTSERRVYRRLTTPEKIQRFLDELRYNKEEHGPTCFSP
                     RRVVRENKAHCMEGALFAAAALEFQGFPPLLVDLEAVRDDDHVLAVFRQRGHWGAIAK
                     SNYAGLRFREPAYRTLRELAMSYFEHYYNDAGEKTLRTYSRPVNLRRFKHIHWETAED
                     DVWAIPEYLCEISHTPLLPGAIERNLRRVDARLMAAGRVGSVK"
     gene            2463..>3370
                     /locus_tag="VLE22_06695"
     CDS             2463..>3370
                     /locus_tag="VLE22_06695"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB14129.1"
                     /translation="MSFDLIIIGSGPGGYSAAIRAGQYGLRTAIIEKYPKLGGTCLHV
                     GCIPTKALLHTADLWEEILHGKDQGILCDNPRLDYPKTLERKNQVVAKHAKGIEFLMK
                     KNKVEWIKGHATLKGGGRVEVQTDAGVKTIEAKNVIIATGSEARMLPGLTPEPDSILT
                     NIEILDMTAVPKTLAIVGAGAVGVEFASIFNRFGTKVTLLEMLPRIVPLEDEEISKEL
                     QRLFKKSGIRVETGAKADNIRKTGGGVRFSVTLADGKKEDMQADRILIAVGRKPNTEN
                     VGLENTRVKLDRGFIKVNEWMQTDEPG"
BASE COUNT          680 a         1005 c         1076 g          609 t
ORIGIN      
        1 ggaaggcgta ggctctcttg cgcttgaaga cgcaagcgac ttcgaggaac cgagcccgac
       61 agaggcgatg aactagtgga acaaagtcct tggccgaagc tgtccgtgtc aggaagcgcg
      121 ggcactcgcg gtttgaatcc aagattccgt accgttggcg tgtcaagcgg agcgataacc
      181 cctagcctag ttaaagacga gcgtcacatc gaccgttttg tcgctctcgt tgatgtctgg
      241 aatcgctttt gtcttcttga ggttgtcgaa gacgttgtcc tccacgatgc gggcaaggaa
      301 gtagtcgggg tactcgatgt tgtagggagc gccgggcttg agcgcccaga gctgccggat
      361 ggcggcctcg ccgtggatgt cgagtccttt gatcgtcagc tttccgaagg tgaatcgctg
      421 gccctgctcg acgtggatga tcacgtcaac cgtcttcgcg tcgtcgtgaa tcgtccggtc
      481 cgtccggagt tccgggctca tgtagccgtt ccggccgagg aacttccgga tgcggtccaa
      541 tccggccttg acctcagtga agttcgcgat ctcgcccgac ttcaagccga ccgccttccg
      601 caggtccttc tccggcagac cagcagcggc gaaaccgacc tctccgagtt tgtagctttc
      661 tccctcgacc acttcgacgg taaccgcgag gccgttcacc tgccgcgctt tcgctgtgcg
      721 gacgctcgga aaggccaccc ggatgcggcc gcgttcctcg tacatcggcc ggatggtggt
      781 atcgaggatt tgcctgaagc gggactcgct gaacggcgca cccaccgcga cgccggcgat
      841 cgcgttctga agcgccgtca ggggcaacac cgtgttcccc gtaaacgtga cttcggcgac
      901 ggcgggcggc gccgcctcgg gacggaataa gataacgatc cggttcggct cgtccaccgt
      961 gagccgcccg atcaccgtct ctttcttgcc ctgtgacgca agatagtcct cgatgacttt
     1021 cgcgtagcgc tttaaaacga cgtccgtggc cgggatattg tcgccgaaaa acgggtcgga
     1081 tttcttgaga acctgggtga gctgatccgg gggtgcgtca agacggtcga agcggatcgg
     1141 gtagacctgg gcgacctcga gaacctggaa tgtggcggcg tagcccttgc cgttagccgc
     1201 aggaccgaaa cggtacgcaa cactttcgaa cacaccggtc gccatgaggc ggtcccgggc
     1261 ggcgtcgaac tcgcgtttgc cggccagttg gccgatcctg agtccggtgg cggcgacaat
     1321 ctgcttccgc gaatatgtgc tgttgccctc aacggagagg ctctcgagcg tccagcgggt
     1381 gtcggtcggc gcgacttgct ttgccggggc ggactttttg gcaggtcccg ggacctgccc
     1441 tacgaccggt gccttctttc gctgggtggt ttgtgcaacg gcgggcgact gccagaacag
     1501 gaacaagatc aagatccagg ccatacttca attctagtaa ggccgacgag ggcatcggcc
     1561 gcggtccagg ggaaccgccc tacttcaacg cgcggcgggc agcgttcagc gacggcggcg
     1621 gagctcgacg acgccgatca caacgaagcc cgccgcgatc acccagcgga ggatcaccgt
     1681 ccaccagggc gcgcccagca tgaccgagcg aacgccgagc gtaaagaacc cggcgccgat
     1741 gcagatgtac cacagggcca gtagccgcgg gcgcattcca ccgaatcata ctctttcaga
     1801 gactcggccc ggcagcgata caataccagt tcccatgcgg cgggacggtt tcaatacctc
     1861 cgagcggcgg gtttatcgcc ggctcaccac tccggagaag atccagcgat ttctcgatga
     1921 gctccgctac aacaaggagg agcacggccc gacctgcttc tcgccacggc gcgtcgtgcg
     1981 cgagaacaag gcgcactgca tggaaggggc cctgttcgcc gcggctgcat tggaattcca
     2041 gggctttccg ccgctgttgg tggacctgga ggcagtgcgc gacgacgacc acgtgctggc
     2101 cgtgttccgg cagcggggcc actggggcgc catcgcgaag tcgaattacg ccggcctgcg
     2161 tttccgggaa ccggcctacc ggacactccg cgagctggcg atgtcgtact ttgagcacta
     2221 ctacaatgac gccggggaga agacgctccg cacgtactcc cgtccggtca acctgaggcg
     2281 gttcaaacac atccactggg agaccgcgga agacgacgta tgggcgattc cggagtacct
     2341 ctgcgagatc agccacacgc cactgctgcc gggcgcgatc gagcggaatc tgcggcgcgt
     2401 ggacgctcgt ttgatggcgg cgggacgggt cggatcggtg aaatagaact aaaatggagt
     2461 taatgtcctt cgatctcatc atcatcggca gcggtccagg cggctattcg gcggcaattc
     2521 gcgcggggca gtacgggcta cgtacagcga tcattgagaa atatcccaag ctcggcggca
     2581 cctgcttgca cgttggatgc ataccaacga aggcactctt gcacaccgcg gacctttggg
     2641 aagagatcct gcacgggaaa gatcagggca ttctctgcga caacccgcgt ctcgattacc
     2701 cgaagacctt ggagcgaaag aatcaggtcg tcgcaaaaca cgccaagggc attgagttcc
     2761 taatgaagaa gaacaaggtc gagtggatca agggccatgc gaccttgaag ggcggcggca
     2821 gggtagaggt acagaccgac gccggcgtga agacgatcga agcgaagaac gtcatcattg
     2881 ccaccggatc cgaggcgcgc atgttgcccg ggctcacacc cgaaccggac tcgatcctga
     2941 cgaacatcga aatcctggac atgactgccg tgccgaagac gctggccatc gtcggggcgg
     3001 gcgcggtcgg cgtcgagttc gcctcgatct tcaaccgttt cgggacgaag gtcactctgc
     3061 tcgagatgct gccgcgcatc gtgccgctcg aagacgagga gatctcgaag gaactccagc
     3121 ggttgttcaa gaaatccggc attcgggtgg agaccggcgc gaaggcggac aacatccgga
     3181 agacgggcgg cggcgtccgg ttctcggtga ctcttgccga cggcaagaag gaagacatgc
     3241 aagccgacag aatcctgatc gccgtcggcc gcaagccgaa cacggaaaac gttgggctgg
     3301 aaaacacccg tgtgaaattg gaccgcggct tcatcaaggt caacgagtgg atgcagacgg
     3361 acgagccagg
//
LOCUS       DATIFQ010000145        52443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_241233, whole genome shotgun sequence.
ACCESSION   DATIFQ010000145 DATIFQ010000000
VERSION     DATIFQ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 52443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52443
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241233"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2315)
                     /locus_tag="VLE22_06700"
     CDS             complement(<1..2315)
                     /locus_tag="VLE22_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HSB14130.1"
                     /translation="MRLTATLALVFCASASAEITVDENATRAKLVNNSTWVFLAIHNQ
                     FEKPVDTRVELEWLDPENTRRGRTQFAFTAKPGSSIAEAALPVNPTDDPLFFRLHYSV
                     SADTRNLTAFSPIRGMISFPIIAEHGFRLTAAGIGVARLGQPYEFHVFASHPITLAPV
                     ADVQITLDDKPVARTGADGTAVVRLMLATDDWEYPDNITFHGRLGDLVQETEAWHPGV
                     MPNDVRICTDKPLYQPGQTVHVRILALGSDGKARSGAEYGIEISDERSTTVHSADLKT
                     SQYGIAHTDWEVPANAANGKYEIELEDKDAGITYQRYVEIRRYELPSFRVTVRLDRSY
                     YLSGQNAIAEVRADYLFGKSLSAGSVRICAAENDTALHEGSLGLGGTFHATLDLAAAR
                     EDLGDARFADLHYVAYVTDAGTNRTEKRPFDVRISRAPIHIYVLSRDRAALWRPLYVM
                     TYTPDGKPAASDVEALVDGQVLGSGKTNRFGLARLSIPPNTSGEITVRARASEGQATQ
                     TEQVWPGGLEVRLETDRVLYVAGQPVRCRITSPRHDLHATLLAWNRRGDVLFSKPLHL
                     KKGSAEAVIPYDARFGREVTIEILSPLGDDYQTGRTILYPGAEDLQIIAAPGAGSYRP
                     GDEATLTVRTASSSGSPARSALGVAVVDESVFERVAGDTANRRHGWFDSGYESRESKI
                     GGFGITDLLNLDPARIDADLQLVAEALLDSQSVAPSGIGTFLELDRQTDYPATVAEFG
                     RLLSEDGLRLDRLKDPWGSPVRVAISHFRDR"
     gene            2436..3344
                     /locus_tag="VLE22_06705"
     CDS             2436..3344
                     /locus_tag="VLE22_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSB14131.1"
                     /translation="MATRRWVFLTGTIAGAALLVLTAASAVFCEGTLRVQKTASGSGV
                     PPKDRYPDALWRTVHIRAKDGVALDGWFIRPNKSTSSRCVVVLHGIADSKSGAAGFAP
                     MFLAEGYSVLLPDSRAHGNSGGEFVTYGLFEKSDVLEWTRWLRKEGCSEICGLGESLG
                     ASVLIQASAEEPVFRAIVAECPYADLKAIAVYRVQKVIRGPQWISSAAAGLIVHSGML
                     YAKLRYGLDFSQVAPTMAIARSKTPILLIHGVQDGRTPCWHSQRLAQANPRAILWLVP
                     NADHVGASSADPDGFRSRVLDWFARS"
     gene            3501..3863
                     /locus_tag="VLE22_06710"
     CDS             3501..3863
                     /locus_tag="VLE22_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSB14132.1"
                     /translation="MTTLTLLGIGLVAGVFAGMFGIGGGLVIVPALIFLLKIKELEAI
                     GTSLAALIPPVGLLGAAEYYRNGFINLRFALLVAAGLFLGAYFGAKIMISLPPNMIRR
                     VYGTFLMLIAIRMLVMGK"
     gene            complement(3866..4330)
                     /locus_tag="VLE22_06715"
     CDS             complement(3866..4330)
                     /locus_tag="VLE22_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14133.1"
                     /translation="MHEVLWITLPFVVALGSGVISFIIAQARMEVYLSKRREALVEAR
                     ATLAFQHKVMEERVKAAEAEARRKALDEFLSDVRVEERHYVREAKSAQERRRSLVLQE
                     RIYFRNIPLSRWVEHEMPVEEEGDLDARLSDASVFHHESLPPKNGVARKLLT"
     gene            complement(4438..5361)
                     /locus_tag="VLE22_06720"
     CDS             complement(4438..5361)
                     /locus_tag="VLE22_06720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB14134.1"
                     /translation="MEEIQPPPKVSAVVASWNNAAALRRCLGALERSTGRENMEVVVV
                     DCGSHDESPLLDAEFPKITMLRLPRNFGIVKALNIGMRTAKGDYYLFLPPEVEVQPDT
                     VAVLVAQLEATAEAAAVAPLLLSPEGDVLTRHRRVPLPDEAYRAWRAGDFADWTTPAC
                     DVDALPVEFLNLAVLMARANFLKGMRYIDERYGNSCWDLEVCCQIRRASKRVLLLRTV
                     SAVLQAAPAAATYPAIRAQFAADRVLGIVTYAGKHFGLGAGMKTRLLATLSALGAAMA
                     SLVTFRDTRYCFSLLSCLLSSQKIDGSQSAF"
     gene            complement(5459..7867)
                     /locus_tag="VLE22_06725"
     CDS             complement(5459..7867)
                     /locus_tag="VLE22_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HSB14135.1"
                     /translation="MKRLLPLLALLPFSLPAWAGAAAELANQIREAGLDRDECYRVRD
                     VNFTKDDLRLYLTDGHLIFGKPVGGRRVSAVFTADVEAGDAEILLFPPTRSERLALAS
                     LTDAPNLNEHFKVAVMVFTDDTCDALLKLIQEREPRKSPERGVLMEQEWGVVARNLSS
                     SFEIRMVRDLLSSRPPSNGFFYAALSGGRLGNFDVVYDPLAREQITVGKVAFRNERAH
                     FDTWTSFESRPFRKGLRQFPPPDIVVSDFRLDATLQPDLSLKVVTKVKVTPTEKGYRS
                     LAFDLSARMRITQALIDGVPAEVFQRESLRENLIHGRRDGLFLLIPPGELEPGRQYEI
                     EFHHEGEVVAQAGNGVYYVGSRGNWYPNRPTQFAHCDAVFRYPTTLDLVSGGEVVEQR
                     TEGEWRITRRRTSSPVRFLGFNLGEYRKVSLNRKPYSVEVFANRKLEPALQPRPRQVV
                     APQHTPSWRRPGPEDLVTLDIEPLPVDPTLRLEGLASEIADAFEFMAEHFGPPPIRNL
                     TVSPIPGAFGQGFPGLVYLSTISYLDPKQRPAAASGGYQQLFFSEILHAHETAHQWWG
                     NIVTSGSYQDDWLMEALANYSALLYLERRKGPDALESVLTEYRRRLLVKSPSGRTTES
                     VGPIIWGVRLDTPDVPWAWRAITYEKGSWIMHMLRRRMGDEKFLGMLGELRRRYEFRT
                     VTTEQFRALAAEFMPPKPADRQLESFFDNWVYATGIPTLQFRHTVRGKAPAWRVAGTL
                     TQSDVAADFGIDVPLEIHLGKGKVETHWLQTDTEPTSFSFTLRQKPVKVVLAPGDSIL
                     AVMK"
     gene            complement(7903..8664)
                     /locus_tag="VLE22_06730"
     CDS             complement(7903..8664)
                     /locus_tag="VLE22_06730"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HSB14136.1"
                     /translation="MIDIHSHILPGLDDGARDMEESLAMLRVAAEAGTTDIVATPHSN
                     LEFQFDPDLVDRKIAELAAASDGVVRIHRGCDFHLHFENIQEAIENPAKFAIAQKKHI
                     LVEFSDLLIARSTEEVFNRLLDAGLVPIITHPERNRLLQQRIERLERWVQVGCAIQVT
                     AQSFFGLFGKSARRFAGELMCRGLVHIVASDAHDSRHRPPRLDEAYRYLESHYGVDTA
                     ELLCVRNPGAVLAGDHIEQPDAASPSARKWYRFWT"
     gene            complement(8716..9222)
                     /locus_tag="VLE22_06735"
     CDS             complement(8716..9222)
                     /locus_tag="VLE22_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14137.1"
                     /translation="MNQDAVFYLMTAAVGVSALAVVVQTLVLFAIYRTARTASEQIRE
                     VGGQVESLAQTTQRTLEQSRKQLTDVTTKAGEVLDLARTQLVRIDDVLAEATTRAKIQ
                     MDRVELVLDDTISRLHETAALVHNGVLKPIKEVNGVAVGIRAFVGALLRGRRLTVEQA
                     THDEEMFI"
     gene            complement(9253..9573)
                     /locus_tag="VLE22_06740"
     CDS             complement(9253..9573)
                     /locus_tag="VLE22_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HSB14138.1"
                     /translation="MGDDRRLSYFFLGLGIGVAVGVLFAPKSGLETRELIRNRAGEGK
                     DYLRKRGEDLYDSAGELIDKGKTAIGRQREQLAAAVQAGKQAYREAVTAPEPGSEGTS
                     PAKG"
     gene            9722..10276
                     /locus_tag="VLE22_06745"
     CDS             9722..10276
                     /locus_tag="VLE22_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HSB14139.1"
                     /translation="MAARKPKKLDSEGLWSYALRSLAGRAHSVSELREKLRRRAEKPE
                     DTAAILARLRERGYLDDRHFAEGFAFSRLGNQGFGKHRVLRDLRQRRVAPAVAERAVR
                     EAYQDTDEVELIESFLARKYRRVPLAEFLSEPKNLASAYRRLRVAGFSSSPVIRVLKR
                     YAAEAESLETLDTEDDPGERRDAE"
     gene            complement(10287..11072)
                     /locus_tag="VLE22_06750"
     CDS             complement(10287..11072)
                     /locus_tag="VLE22_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSB14140.1"
                     /translation="MVRPIPGYTLLELIQKAETESVSLSELAGQSASRHAEQGTAVIP
                     LLPLEVWACGCTYEVSASFRDGEQGAQEGIYAHVYRESRPEIFFKGTARVCVGPCKPI
                     GIRADSKFTAPEPELALVLGRRGRILGYTLANDVSAWDIERENPLYLPQSKMYTSSCA
                     LGPVIVTVDELVEPYQLEMTCTITRGDSTIFSGSVSTSRLHRRFETLVEYLLRANPVP
                     AGSVLLTGTGIIVPESAALQPDDVVSIRVPEIGELRNPVALVE"
     gene            complement(11140..12579)
                     /locus_tag="VLE22_06755"
     CDS             complement(11140..12579)
                     /locus_tag="VLE22_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSB14141.1"
                     /translation="METFRNYINGEWVEGTTFENRNPANVDEVVGLFVRGSATDVEAA
                     AGAAEAALGPWSSLPAPARGNYLYKTAEILDRKFEQLAAEMTREEGKTLPEAKGEVRR
                     AINIFRYFAGEGSRLPGYLVPSERDRVHMFAVRKPVGVAGLITPWNFPSAIPAWKLAP
                     ALICGNTAVIKPASAAPLSAWRIVEACHEAGVPKGVVNFVAGPGGELGKTLINAKPVK
                     AVSFTGSCEVGHWLHGEASKRRLKIQLEMGGKNPTIVLADADFALAVENVVNGAFFST
                     GQKCTATSRAIVEESIYNRFVEALVERTRKLRVGNGLEPGVDIGPAIDQAQLDTDLRY
                     IEIGRKEAGPPRIGGERLTGDGYDKGYFVAPTIFADVTTDMTIAREEVFGPVLAVMRA
                     RDFEDALRIANNIPFGLSASLQTTNVSRVFDYVYHSEAGLLTVNLPSAGVEYQLPFGG
                     TKDSSFGPKEQGPTALEFYTDFKTVYLKY"
     gene            complement(12691..13257)
                     /locus_tag="VLE22_06760"
     CDS             complement(12691..13257)
                     /locus_tag="VLE22_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSB14142.1"
                     /translation="MKAVAIVLGVLLLAGLIFGGKLVGIRNELVTQRESINGAWAQVD
                     VALQRRADLIPNLVETVKGFAKQEREVMESVTSARAALLGARTPQEKIDANTRLDGAL
                     GRLLLVVENYPNLKSNENFLRLQDELAGTENRIAVERRKYNETVQRYNTTIELFPNNI
                     AASLFGFARNDAYFKTEPAARTVPKVAF"
     gene            complement(13268..13978)
                     /locus_tag="VLE22_06765"
     CDS             complement(13268..13978)
                     /locus_tag="VLE22_06765"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HSB14143.1"
                     /translation="MERVLNQLVERLRKAYDDRLVSVVLYGSAAVGDHHGRFSDLNIL
                     CVLRLVTSRELADSAPIFRWWRELGNPSPLLMSLDEVRTSTDCFPIEFHDIRGRHRIL
                     SGEDIVKDLEVDKSFYRAEVEHELRAKLLRLRQKAAGVLGDKELLRSLLSDSLSTFCV
                     LFRHALLLAGAESGFEKRQVIAKTRERFGIDPSPFEAILDLREGKRKPRDVDPSPLFE
                     SYLVQVQTVINAVDQLEK"
     gene            complement(13991..14776)
                     /locus_tag="VLE22_06770"
     CDS             complement(13991..14776)
                     /locus_tag="VLE22_06770"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HSB14144.1"
                     /translation="MRRLSLAACALMVCAAAAYPVDFAALKPEGYVSDFARVIDPGSR
                     ANLERYCSRLDTQTGAQVAVVTLSTLAGEPIEDVANDMFRRWGIGQKGKNEGLLLLLV
                     IQDRKMRMEVGYGLEPIIPDGFSGSILRAMRPALREGRYGEALTEAVQVAGSRIAEAK
                     GVKLDTTLPRRTRPSASEIPWPVIIGGVVMLLLLLNGVGGGRRRGRGSGADLLLAMLA
                     GNIMGRSMGGRSSGGGFGGWDSGDTFGGFGGGDSGGGGASSSW"
     gene            complement(14830..16116)
                     /locus_tag="VLE22_06775"
     CDS             complement(14830..16116)
                     /locus_tag="VLE22_06775"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSB14145.1"
                     /translation="MSSSNLSVRDIEVSQLPSGIRLISETIPHVRSVAVGIWMGTGSR
                     QESAEENGISHFVEHMVFKGTTNRSAEEIARSVDSVGGNLDAFTAKELVSFNAKVLDE
                     HLPLVFDVLSDLVLNPVFRDEDVEKEKGVVLEELKMEADNPEYLVHEVFSGNFWKDHP
                     LGKPILGTRETVKRFGRDMIETYYRSVYVPSNITITAAGNLTHSQLAGLVGERFGALS
                     GNGSVPATPPASTHARISLRNKKSLEQVHVCLGVPCYPLPHEGRYVSYVLNALLGGGM
                     SSRLFQNIREKRGLAYAVFSELSAYRDTGCLSIYAGTSLETCRQVIHLIIEEFKQLKQ
                     ELVGEEELRRAKDHLKGSMMLGLESTSNRMANLARQALYFGRFISMDEMLNSIEKVTA
                     AEVQQLAQDIFDPRHIALTVLGNLEGLRVGREELAC"
     gene            16226..17266
                     /gene="rlmN"
                     /locus_tag="VLE22_06780"
     CDS             16226..17266
                     /gene="rlmN"
                     /locus_tag="VLE22_06780"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HSB14146.1"
                     /translation="MAQVLLGMELPEIQALLGPGHPGYRARQIYDAIYRQRATGLSAI
                     SNLPRALRHMLQTDWRIGLPALETRFASTDGTKRYLLKLDDQRTVEAVLMPEGARSTI
                     CISTQVGCPVDCKFCMTALMGLERNLTAGEIVGQVLFVAGNTDLWAGLDRLNIVMMGM
                     GEPLLNLPNVLKATRLLCDPDGIGLSVRRITLSTSGIIPKIEELGRADVRPKLAISLN
                     ASTEEQRRALMPITRKYHLKDLMDACRAYPLRPWERLTFEYVLLRGVNDSDADARRVV
                     GLLANLKSKVNLIALNPGPGIPFETPEPERVESFQTIVKRSVPCFVRKPRGRDIFAAC
                     GQLKRVALAEIA"
     gene            complement(17283..18095)
                     /locus_tag="VLE22_06785"
     CDS             complement(17283..18095)
                     /locus_tag="VLE22_06785"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HSB14147.1"
                     /translation="MKLARFMFILLCSLLVAGTLPAQSKRKKLLAIGEVKGFQHDSVS
                     HALGTIWKIGQESGLYDTYIRTDTQLITKKKLDGNAKNLDFFDAIFFYTTGELAMNEQ
                     QKADFLSFIRDDGKGFLGAHSANDTFYKWPEYGEMIGGYFDLHPWNQFQAPIIVEDRN
                     FPGMQQFPPTFTIHDEIYQVKDFSRERVRVLMRLDADKVDLKTKGVHRTDKDFAVTWA
                     RNYGKGRVFYCGLGHREEVWDRPDVQKMWLEAIKWTMGLIPGDATPRPMPVK"
     gene            complement(18181..18999)
                     /locus_tag="VLE22_06790"
     CDS             complement(18181..18999)
                     /locus_tag="VLE22_06790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB14148.1"
                     /translation="MSVNGRTVLISGAGRGIGKRLAIGFAAAGARVGLLARSQPELDL
                     ARLEIAQAGGSAVSLPADVRDFDQVCGAVERLREEFGPVAVVVAAAAVQGPIGPFHTA
                     DPRQWAEAIEINLFGVMHTCRAVLPEMIQRRRGKIVVLTGGGTTRPRTNFSAYAASKT
                     ALARFVETVAGEVLNFNVQINCMSPGGTYTHMTDEILHAGEDAGAKDYEDARQVRETG
                     GVSPEQQIQLALFLASDRSNHVSGKIIHVKDAWKRLENSNSDSELFTLRRMVRR"
     gene            complement(19086..20033)
                     /locus_tag="VLE22_06795"
     CDS             complement(19086..20033)
                     /locus_tag="VLE22_06795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSB14149.1"
                     /translation="MPSGNDPSNDPNQQERLFHESLSRYVSSLDEIARRASDAGRELV
                     STYARQIESIRGCDGGDGEAASSAESRNAFERAFLEYSRKVDAFYGQQQEELRRIFAD
                     LAVAGEAAPAAEPTSVPGGLSHLWAMVDVMWSESQATVRQLRAELQMFQRRLEAAETL
                     ASTDPLTGLANRREAERIIGEMARSERRFSIMLFDLDRFKSVNDRYGHSAGDQLLRAF
                     ARRLLEQFRPEDLVCRWGGDEFLAVLSSESGDAKARTRQIADRLGGRYSVKCAGRELV
                     IDVGVSAGAAEYAPGEVAEELFARADVLLYKCKGEECAS"
     gene            complement(20118..21215)
                     /locus_tag="VLE22_06800"
     CDS             complement(20118..21215)
                     /locus_tag="VLE22_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB14150.1"
                     /translation="MQRRTFLQTLSAIPASAAAGKSEAPAPAAGIPLGYDTYSLRSFG
                     WKAVELLDYAAKLKLDTIQISSVGNYESLDPAHLARVKSHAERLGIAVEGGTGCICPT
                     SASWSAKNGDPAEYTLQGLRVTRAAGGHVMRCYLGSGADRLGSIPIEGHIESTVKVLR
                     AVRQQALDLGVKIALENHSGDLQAWELKMLIEEAGPEYVGACLDTGNPIWAVEDPMVT
                     LEVLAPYVLTTHIRDAVVYEHPRGAAAQWVALGDGSLDFRAFVARFRELCPKVAMQFE
                     IITGRPPRILPYLEPDFWKAYPKARAAEFARFVALAKNGHPFMGTMIIADSGDPPPEF
                     RAALREQQRVDLERSVNYAQKVLGAGIRWRA"
     gene            21318..22286
                     /locus_tag="VLE22_06805"
     CDS             21318..22286
                     /locus_tag="VLE22_06805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSB14151.1"
                     /translation="MSSAESFLSEWIGRSGPVTFHEFMEAALYHPEFGYYRRARDPFG
                     REGDFYTAEQLQPVFGILIAARIRSLFEEMGRPPDFRVVELGAGRGEMAKELSSFHYV
                     SVEIGDPMPERFTGVVFSNEFFDALPVHVAVRRGTSWNEMRISRRDQCFAWEECGEAP
                     EEVRRYLDRYAGSPEEGNLCEANLDALRWLEEIAARLERGYVFTIDYGYTARELIRFP
                     HGTLMSYHRHTAAENVLTGAGERDITAHVNFTALRDHGERSSLETIRFESLARTLLDA
                     GEADQFAAALSSGGEAERMRRTLQLKTLLYGMGETFRTLLQKKAAK"
     gene            22611..23309
                     /locus_tag="VLE22_06810"
     CDS             22611..23309
                     /locus_tag="VLE22_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HSB14152.1"
                     /translation="MVCPRIFGFRRSLSPAIVAVIVAALVLLTACARKRPARPPIAPV
                     PGALEFGVASWYGHPYHGRRAANGEVYDMEKLTAAHRTLPFDCWVEVLNLRNNKSVQV
                     RITDRGPFVDGRIIDLSRAAARQIEMIGPGSAEVRLQVIAPPQAPVAENVFAVQVGAF
                     QDRGNAERLAGEMKQRFGESRLVQRDGSPRLWRVLVGREPNLEDANVLAERIRAELGE
                     AFVVRLDSPAGDRL"
     gene            23322..24305
                     /locus_tag="VLE22_06815"
     CDS             23322..24305
                     /locus_tag="VLE22_06815"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB14153.1"
                     /translation="MFAVRADSPPVLVGQSFRIRQVLQLIQKLGRSRSPVLVLGETGT
                     GKEVVARAIHHVVPAGPFVPIDCSALVGPLMESELFGHTKGAFTGAATAKIGLVEAAN
                     GGTAFFDEIGELPLDLQAKLLRLLQEKEFRPVGSVERRKADFRVIAATNRDLVQEVER
                     GRFRRDLYYRLNVITLRVPPLRERKEDIPALVGYFLRLHGGNCSVSRECLDAMIAYDW
                     PGNVRELENCVQRMSAVNSGPLLQTADLPTALHTYMEARRAGSLVMTAVAVADVSVAP
                     APPVLSLSEVEKRTIREALDYTKGDRVMAARLLGIGRTTLYRKIKEYRLTD"
     gene            24302..25330
                     /locus_tag="VLE22_06820"
     CDS             24302..25330
                     /locus_tag="VLE22_06820"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSB14154.1"
                     /translation="MRIALDATYSVGDQLSGVGVYSREMLLGLAQAHPESCFLFCFRP
                     HRFLRSLRLPVPRNASRRILIGERPPRLADLFHGLNQRLPHGRHRRAVSTFHDLFVLT
                     GEYSTAEFRRRFAAQARDAAERSDLIITVSAFTAGQVEQLLGVARESIRVIPHGVRLP
                     AGDRVAREKIILHVGAIQKRKNITRLVEAFENTGPDWRLVLAGSAGYGAEDVLSRIAA
                     SRRRTDVKVLGYLPSAGLADLYRRASVFAFPSLDEGFGMPVLEAMASGVPVLTSNRSA
                     LPEVAGDAALLVDPLDSQAIGNALRRLTVDDQLRRELIERGLRRAVGFTWEESVRKTW
                     AVYEELLG"
     gene            complement(25334..25621)
                     /locus_tag="VLE22_06825"
     CDS             complement(25334..25621)
                     /locus_tag="VLE22_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14155.1"
                     /translation="MQELGNAINESLSESDRIAAAIGEIKRAGYEVFLVLEATIGFNK
                     KEDTVEGESATIDETFEAPAGEFEAKGKVKWTLQDQKFLRALKISVDEDAH"
     gene            25782..26045
                     /locus_tag="VLE22_06830"
     CDS             25782..26045
                     /locus_tag="VLE22_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14156.1"
                     /translation="MANIGREFVRHVLPKILKPMRALWNEMIGFVFLALAAIPVPRTF
                     RMWQEYDEAGEGLFKIVLSGIFIAIMAVFGVHSFLRARRITRS"
     gene            26042..27550
                     /locus_tag="VLE22_06835"
     CDS             26042..27550
                     /locus_tag="VLE22_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSB14157.1"
                     /translation="MSDPRGKPGQFPYTRGIYPDMYRGRLWTMRQYAGFGAAEESNER
                     YRYLISQGTTGLSVAFDLPTQMGMDSDHPMAAGEVGRVGVAICSLADMERLFEGIRLD
                     AITTSMTINSTAATLLAFYVLVAKRQGVDVRRLSGTVQNDILKEYVARGTYIYPPRPA
                     MRIITDLFFWASSELPEWNTISISGYHIREAGSTAVQEVAFTLSNAIAYIQAALDAGL
                     AIDTFAPRLSFFFNAHSNFLEEIAKFRAARRLYARIMRDRFGAKRPRSMTLRFHAQTG
                     GSTLTAQQPDVNIVRVSLQALASVLGGAQSLHTNARDEALSLPTEESVRLALRTQQII
                     AHESGVTQTADPTGGSAFIEELTDRIEREAQLYIARIDEMGGTLRAIESGYIQSEIQN
                     AAYEHQRAIESGELVVVGVNRFQMEKRAIPIFRLDPELEKRQIQRLGELRAGRDKGKL
                     EGSLDRLEAAARGEENLMPFILDAAAAYATVGEISNRLRKVFGEYRETGAST"
     gene            complement(27578..28780)
                     /locus_tag="VLE22_06840"
     CDS             complement(27578..28780)
                     /locus_tag="VLE22_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14158.1"
                     /translation="MFHADFGRRDLFRFAGSALAMTASSYSRVLGANDRVHLGLVGCG
                     GRGRSVMGTFIETGSVQVDAVCDIYGDRIDRALQKAAGATSHRDHRKLLEMKELDAVL
                     IATPDHWHALTAIDALNAGKDVYVEKPLTLKIEEGPEIVKAARVNNRICQVGMQQRSG
                     PHYIQAKEEYIKAGKLGKITLARTWWHGNGAHLMKAPMAEKPSNLDWARYLGPVKWRE
                     WDPPQYFNFRAYLDFGGGQVTDLFTHWIDVVHMFMDQDNPTAAVASGGVYHYKDGRTA
                     PDTINVLLEYPAEWTATFEATLAPGITGAGVEMCGTEGRLFITRSRFEFTPVGKGAQP
                     VVVKSERDQTIDHVENFLECCRTRKLPNGDVYIGHRSAQASHLGNIAYVQKRRIKFNP
                     DREEILPL"
     gene            28835..30169
                     /gene="trmFO"
                     /locus_tag="VLE22_06845"
     CDS             28835..30169
                     /gene="trmFO"
                     /locus_tag="VLE22_06845"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HSB14159.1"
                     /translation="MTRSIHILGGGLAGTEAALQASKLGVPVVLHEMRPAVPTAAHRT
                     DRLGELVCSNSLKSEQESTAPWLLKQELRRLGSVLLEAAAKARVPGGHALTVDREVFA
                     EEVTQAVASDPRIELRRDEVREIPRGEIVIVATGPLTSDALASDIAVLTGSERLYFYD
                     SISPIVEADSIDMSVAFRASRYDKSLDSSGDYINCPFDREQYERFIDALLEAQQHQPH
                     ISADVPYFEACLPVEELARRGRDTLRFGPMKPMGLTDPRTGNRPYAAVQLRQENLRAD
                     SFNMVGFQNYLRYGEQARIFRMIPGLECAEFLRYGQIHRNTYINGPALLDALLRLKAH
                     PGIFFAGQVSGVEGYVESIATGLVAGRNAASLALGEEPRPLPRQTALGSLCAYVSGAD
                     LDNYQPANITFDLLPPLDDATKRKLRHDKRARRVEVCRRALEALEEHLGVHV"
     gene            30162..31055
                     /locus_tag="VLE22_06850"
     CDS             30162..31055
                     /locus_tag="VLE22_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HSB14160.1"
                     /translation="MSELAQQIQRFLDSRRRENVSEHTLRAYGADLKQFLEYFSPQGA
                     EPPALAGLDLLLLREWMADLYARKLSPVTLRRKLAAVRSFFHFLHREGLIGMNTARLL
                     GTPKTPKTLPRVPSAEQTNRILDGAGGQTERPSLARDLAILEMLYGCGVRVSELAGMS
                     LDDIDFGEQWVRVRGKGRKERQVPIPSKAAAAVERYLAERVAPPGERAVFVNRRGGRL
                     TDRAVRAIVKFYATLVAGDSSIHPHTFRHAYATHLLSDGADLRAIQELLGHTRLSTTQ
                     KYTQVSLTELMAVYDRSHPKA"
     gene            complement(31009..31989)
                     /gene="modA"
                     /locus_tag="VLE22_06855"
     CDS             complement(31009..31989)
                     /gene="modA"
                     /locus_tag="VLE22_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSB14161.1"
                     /translation="MTTGCPPSMTATTEFVVPRSIPMILLIFLKSSSETRYTLNSMKH
                     FGMPYRSDPACVSTFPLSNLSAPLSRRLSSFIVLWLTLLLLLGQSLTAGVTVAGAADL
                     APLGNSLKAAFERATRIRVTFTLASSGMLARQIEHGAPYDVYLSANQKFVDDLVRSGH
                     IRQDSVRIYAHGRLGLWSRSGSVRTLQDLTKPEVLHVAIANPTHAPYGMAALNLLKES
                     GLFEAIRGKLVYGENVRQAFEYAASGNADATITSWTLLHNKEGILLPESWHQPIRQAG
                     GIVAATAQPDAAKRFLDFLVSEEGRRLLEQGGLFPPTPSDASDRKPPLTR"
     gene            31910..33820
                     /gene="dnaK"
                     /locus_tag="VLE22_06860"
     CDS             31910..33820
                     /gene="dnaK"
                     /locus_tag="VLE22_06860"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HSB14162.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGQPVVIPNQEGGRTTPSVVGFAK
                     SGERLVGQVAKRQAVTNPENTVYSIKRFMGRRVGEVTEEMTMVPYKVVAGENSDARIE
                     IQSRRYSPPEISAMILTKLKEAAEAYLGEKVTKAVITVPAYFNDSQRQATKDAGKIAG
                     LEVMRIINEPTAAALAYGLDKKKDETIAVYDFGGGTFDISILEVGDGVVEVKSTNGDT
                     HLGGDNIDQRIIEWIVGEFKRENGIDLSKDQMALQRLKEAAEKAKIELSTLLETELNL
                     PFITADATGPKHLVMKLTRARFEQMVEDLLNRSVGPCKQALADAGMTPADIDEVVLVG
                     GTTRTPKVQQIVRELFGKDPHKGVNPDEVVAVGAAVQGGVLGGEVKDVLLLDVTPLSL
                     GIETLGGVFTKLIDRNTTIPTRKSETFSTAADSQTSVEIKVHQGERAMAADNRLLGVF
                     QLVGIPPAPRGIPQIEVTFDIDANGILNVTAKDRATGNEQKITITSSSGLGKEEVDKM
                     ARDAESHAADDRKRKDEVEARNRADSMVYQTEKLLKEHRSKISDADARDIESALEETK
                     RAVREGTVELINSATDRLTTASHKLAEAMYRATAEQASSAGPAQEQPAEPPKGKENVV
                     DAEFVDVDDKKK"
     gene            33884..35035
                     /gene="dnaJ"
                     /locus_tag="VLE22_06865"
     CDS             33884..35035
                     /gene="dnaJ"
                     /locus_tag="VLE22_06865"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HSB14163.1"
                     /translation="MASPDYYETLGVARGAKPNDIRKAYRRLARKFHPDLNPGDKSAE
                     ERFKKIQEAYDILGDPKKKQMYDQFGFYSASGAPGGQEGFRAGPEMGFGGFDFSDLFS
                     GAGSRATGGSSNAFRDMFSQFFSRGAHGTEARGPERGSDLEYGLSVDFWQAIRGTQVR
                     LDINRQEVCDVCGGSGKHGGQSAVCPECNGSGTVTQIAGAMRFSLSCPRCGGQGRLRD
                     SCEKCHGDGRLSKAETVEVRIPQGVKSGDRLRVPGKGNSGAGGGAAGDLYITVRVEPH
                     PFFQREGDDIEIRVPVTVSETGLGAKIEVPTIDGRALLKVPQGTHSGQRFRLREKGVF
                     NARKGVRGDQIVEVLVQSPKVRDERTKEILRELATLNPEDPRAELWSKV"
     gene            35039..35482
                     /locus_tag="VLE22_06870"
     CDS             35039..35482
                     /locus_tag="VLE22_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSB14164.1"
                     /translation="MPKRRSKAAYMISAVAEQYQLHPQTLRLYEREGLLKPSRSEGNT
                     RLYTDDDLERLEVILQLTRELGVNLAGVEIILNMREKMSAMQRQIEEFVATLNREMAT
                     HFHSPEPAEHTSLIPVIHLAPLVVQNAPRRRKRRSRDEDSAAVAG"
     gene            complement(35492..36118)
                     /locus_tag="VLE22_06875"
     CDS             complement(35492..36118)
                     /locus_tag="VLE22_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14165.1"
                     /translation="MKWHSKVPFLLALVLLLAGCSPAPKPDVQKAKEPEKPADPITGQ
                     KAFYQMYGMARQWAPDVQVLRVRSIPISEVKSAAGASGAWEATFVSQSRGRARSYTYS
                     VVESAGNLHKGVFAGLEEGWSGPRGQAKPFLVAALKTDTDKAYEIALKKGADYAKKNP
                     EMPISFILEWTPRFPDPAWRVIWGDSVASSNFSIFVDAVTGEYLQTMR"
     gene            complement(36177..38228)
                     /locus_tag="VLE22_06880"
     CDS             complement(36177..38228)
                     /locus_tag="VLE22_06880"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HSB14166.1"
                     /translation="MDALTQAILRLLRRMDAVERRLDDVEASLGISRAVPPEPAPTLE
                     PVPAEEQPVLEEAAEPGSPGPAPAMTSSSEGLETRFGLTLINRVGAVTLTLGAAFFFK
                     YAVDNQWIGERGRILLGILAGLLALGLGHLLFRRGHRVYSQGLSGTGVAILYLSFYAA
                     FGFYGLISQPLAFLLMASTTLMAGAIALFYQSRAVLVLGLIGGYATPLLLSTDEDRPW
                     FFLGYVLLLSAGAVAVSKSRKWRGIEAMAFFATAVLFGLWLEDRLSNEKRFVATVYTV
                     AYYLLFSTARGRVVLAAAQFLAAGAMYAIWTPNTAYLIAALGLCAAGFVVAERRERGY
                     LSLISFAGFWVWYAAWRGGGQSPVQTGAVFELLTVAFFLHFAWLPWRVFARRAGPGKV
                     EMLLAALSGTVYFAASYQILSADGSSWRGLLAAALGVLHLALGLRLRPMAGTSGVAGV
                     LCAGLGLGFLTLAIPIQFAGYRITMAWAVEAAALAWIATRVRERRWLHASMVVFALTA
                     ARLLFVDAGLYRDPAAYHAVANLRFLTFATAAASLFLAARWTRSGPYVLASYVAAHAV
                     VLWAFALEVFGWAARSATADNLPSLRSTSLSILMAFYALALVTAGVLATSRINRLLGL
                     GLLAAVVLKLYLYDVWLLRRIYRVTAFAVLGVLLLLTSYLYSRYRATIEAWWKDDGKG
                     S"
     gene            38389..39690
                     /locus_tag="VLE22_06885"
     CDS             38389..39690
                     /locus_tag="VLE22_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14167.1"
                     /translation="MKQQQVLRAAVGLVLAAGLALGQRSGQTQPPSGGAGGNTGGTIT
                     PTPTPTPTPSPRPDSRDRFPQPGEQQQQPTFQEMRRPVFLTGKVILEDGTPPPDSVTI
                     ERMCNGLARPEGYTDSKGRFSIELGRNTMMMADASVSGANDPAFSGMGGLSASRGGFG
                     STSNPAGGLSERDLMGCELRAALPGYRSDAVMLTGRKLLDNPDVGTIILHRMGNVEGT
                     TISATSLHAPKDARKAYDKGREALRKQKWADAQKQLEKAVQVYPEYAAAWYELGRSLE
                     SQEQLDKAREAYAKSLAADAKFVNPYLQLAGLAARDKKWQEVADTTDRVVRLNPVDFP
                     GAYFFSSIAYYNLKNLDAAEKSAREGIKADPQHRFPKINHILGIILAQKGEFTAAAGF
                     MKDYLKFAPKASDADVVKNQLAELEKFAAAQQPAQPPAPQQ"
     gene            complement(39726..41021)
                     /locus_tag="VLE22_06890"
     CDS             complement(39726..41021)
                     /locus_tag="VLE22_06890"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB14168.1"
                     /translation="MAEFLELLRRNRNYRYTWLGQVVSETGDYFNNIAVFGLVMETTG
                     SGLVVSGVMLSRAIPAVIAGPIAGVLLDRFDRKRIMIASDLVRAVVALGFILTIRSHQ
                     SWPLYLLSALLMFASPFFTSGRTAILPAIASKEELHAANSLTQTTQWATLTAGTLLAG
                     FSAARFGYQWAFIINSLSFLCSALAISMLRGGGRDFRAQRYALTEADVVRPWREYADG
                     LRYMRSVPLIFGIGMISVGWAAGGGAAQILFALFGEQVFGRGPEGIGTIWGFAGVGLL
                     LGGAIGHVVGKRATYGGYKRTVTISYLSHGSTYIVFSLMQHYGAALLFVMLSRVGMAV
                     TSVLNYSQLLRHTPDQFRGRVFATMESLRWSTMMLSLAAAGIASQYFSPRAIGVVAGI
                     LGCVTAAGWAWADWRGWLTEPAEAGVRPEEVEIHGDPAS"
     gene            41166..43349
                     /locus_tag="VLE22_06895"
     CDS             41166..43349
                     /locus_tag="VLE22_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HSB14169.1"
                     /translation="MPAHPAERQAGSLIAVTATFTAELVRETLEFWTEQLGLDLSVRF
                     APYNQVFQQLLDPAGLPARNRDGVNVVLLRFEDWVRFRNGFPVNSEDLEQNVRNLVLA
                     LRTAATSVASPFLVCICPASPVFLSKPERRGLLVNLESLLASSVADLGMVHVTTPADL
                     DALYPVRDSYDPHGDELGHIPYTPVFFTALGTFIARKIHAMRRAPYKVIALDCDDTLW
                     SGICGEDGPEGVIIDAPHRALQDFMVAQRDAGILLCLCSKNNEEDVIETFRVHPEMPL
                     RLDQFVARRVNWEAKSVNLAALADELELGLDSFIFVDDNPQERAEVMADRPEVLTLPM
                     PAEADEIPLFLKHVWGFDHVRITEEDRRRSALYEQRAERARLEKQASSLEEFISSLQI
                     DIRIGPMSEGQLPRVSQLTRRTNQMNMTTIRRSESEIQALIESGQAECLTVKVSDRFG
                     SYGLTGVVIFTQRPDTLGVDTFLLSCRVLGRGVEHRVLARLGEIASERGLRGVDVPFV
                     STQRNRPALLFLESVGAHFKESTDGRLLFRFPAGYARGVVYKPAGPPARSAPQHVAEG
                     RKPEAREHVDYGRIATELREPAAIMNRIRSTKAVPEIAAVDKTQPTDLEERLVRLWAE
                     LLRLPSVGIHDNFFDLGGHSLLAVQLLSRVRQEFQVDLSLEVVYSSAFTVAELAKAIE
                     LSQIEQAGADYSAILAEIEGLSDDEVRNLLEREQHRAPGRKDEAR"
     gene            43353..44669
                     /locus_tag="VLE22_06900"
     CDS             43353..44669
                     /locus_tag="VLE22_06900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB14170.1"
                     /translation="MRILLAANASYVPPRGGATRSNLVWLEHLAAAGHSCRVVASELA
                     HDRVGKLEQIRNEEMRVVVSQLDAADGVEVVRHGSVLVFSSADPARRGRLLREQIRGF
                     QPDWVLVSSEDLGQVLLREAVHEAPGRVVYLAHTPQLFPFGPASWNPDPNGAELVRLC
                     AGVVAIGSHTAEYIRQHSGCEVMVIHPPIYPAGPLEKFGNFDEGLITLINPCAVKGIT
                     IFLSLARQCPEYWFGALPGWGTTATDRDALDSLPNVTVLANVKQIGQVLRRTRVLLMP
                     SLWYEGFGLSVMEAMLRGIPVLASDSGGLVEAKMGTRFALPVRPIARYEAVFDEHGLP
                     MAVVPEQDVQPWVGALRGLLSDRILYEDESETSRERACQFVDSIRPEQMEEYLRSLTH
                     KPAERLRSAMVTSGPHDALRHLSPEKRALLLQRLRHKASAVRNGVH"
     gene            44680..46734
                     /locus_tag="VLE22_06905"
     CDS             44680..46734
                     /locus_tag="VLE22_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HSB14171.1"
                     /translation="MRILLAHNSLYYPSHGGGDISNRLLMEALATRGHACRVVARLST
                     FSEDEHRRFVRDLAARQVELKSSAAGAAVFDHRGVEVHVLTENPNLRGYFAGQVREFS
                     PSVILASTDDPAQLLLEASLLADGPRVVYLVRATLALPFGPDAAFPSELKAQMLRNVD
                     GVVGVSRYVAGYVRRWSGIEAIHVPISLMEPGPFPDLGRFDNEFVTLVNPCAVKGISI
                     FLALAERMPGVQFAAVPTWGTNTQDRAALQAYANIAVLDPVDDIDEILKRTRVLLVPS
                     LWAEARSRIIVEAMLRGVPVIASDVGGIPEAKMGVDYLLPVRPIERYQPKVDEQMVPV
                     AEVPRQDIGPWEEVLRRLVTDRRHYEQLSRASHEAAVAYVENLSVAPLESYLEETVRA
                     PARPRLFAPVETAAPSPVDKLSPEKRRLLALRLQRTVSAATPSDSWFPTAVWRERKRL
                     RLFCFPSAGGGTSSFRGWSESLPPEVLICPVRLPGRESRLSEPPFRSMETLIAELAKA
                     IEPYLSQPYAFFGHSMGAAVAFELVRRLRRQGLPPPAGLFVSGARAPQFRLGHVPAPE
                     PSDEEFLDELRRLEGVPAAILENRELLQVILPALRADTELYRNYVYVEEPPLACPIRA
                     YGGVGDPNVKPEHLGAWGRQTTVGFSLRTFDGGHFFVDSARDEFLRALAGDLGELVSS
                     AS"
     gene            complement(46718..47692)
                     /locus_tag="VLE22_06910"
     CDS             complement(46718..47692)
                     /locus_tag="VLE22_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14172.1"
                     /translation="MIEIDPLDDSVLERRDLFRGAGTVAAWLAAANQGVAQAEPADRV
                     PEAQAGPMLPTVPFGKHKLTRLIAGANTIYGYSHFNYVFSTTMGEYHTTPRVLSFLEQ
                     LERAGVNTWQASWSERLEIDWLKYKEKGGKLQLLILSRPNFNEQPEMVKRAMKLQPLG
                     IAQHGGSTNRLWHSGQFEKSRDYLKHIRDAGAMVGLSCHNPKAVEYAEEKGWDVDYYM
                     TALYYMIRPRAEFEKILGEIPLGEIYLASDPPKMMETIRKTPKPCLTYKVLAAGRTVD
                     SPKQIRERMAVALNGIKPTDPVIIGIYQRFNDQIGQTAQFVREIMTQS"
     gene            complement(48027..48383)
                     /locus_tag="VLE22_06915"
     CDS             complement(48027..48383)
                     /locus_tag="VLE22_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14173.1"
                     /translation="MSAFAGQRDPDPTAVAKQMEVRHSILKVSQASAERWVEGFWRSF
                     AESTTSLENSAFEGGIMLDPTIYRLEYSQRFNTLSFSFQDVEIGRESQGNLPVIRWMN
                     GVRREIEGMLAERSQP"
     gene            49280..52255
                     /locus_tag="VLE22_06920"
     CDS             49280..52255
                     /locus_tag="VLE22_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14174.1"
                     /translation="MSALLALATVLAAGANQPEKVVPAINDVEIWQQVGQQPYEFTWV
                     QREEHPQTLVDFEDLKDWRLEMFDGGSGEFRRSREQQLWGQYVGKFLYSGSRPGSRIV
                     ARPPKPIPIPGKFDSIDIWGYGHRWGRRVEQVTPRVDISVWLVDARGKEFKFFMNDVK
                     WRQWWLLHRRIPKEVQAQMLMPASFAGIEVAMGETREPRYFYCDSLAFYVEDLKPLAL
                     KPQPRRNLKPFRGQIAGLNTGPDKLPFPTREETILPSNFEKDFQVSVRQTGPAGFELQ
                     YKGRDATTTYNYRPRSGSLSELSAAIDGGKPFQPLHGGGVRFTDTPKDRVSEGELLSA
                     ELAGDVIKAKFRHGSRIVDYELRLWQKSIVLDVWCDGGEAVELSFGRIAGVTNPKIVP
                     VPYISYRYTNPRVLMSGSETKPLFTSVWFDWYRSNASEPYCSPKPLLTADGGEANGGM
                     RYIPKTDGLRNNLYERVFITSSPVYEETFPTIANPPSLAQEKGKQVVWTVTAPPTFQS
                     DHKRSREIRSYGMDKIMQHSHEVTWRDDGDSFTMRLKAAPQKGGDAMLRWYLKAQNDM
                     GWTQGVYTNYTDFAPVNTNWSPDHVQRLPDGEWRRAWPRNYALKPSKAVEFDEYYAQR
                     IKRKFNPMMSYTDVHTAVPPWEYCDFDARVPGAGTFAATFYAYGQLLLNDQKEYGVCQ
                     TEATFQWLYAGLDSGSYGWVYSDINILGHPLDVAFRLHKIHPLHCDYGIGAPDRHLTE
                     LDPGWRQSPKRRQYIDLFLASTIAYGNMGWLVKDFFDERPFGIEVMARSYYMMQQLQQ
                     QYAYVPVAKIEYADGAGRFVTPSQAQAGGALKDSKVHVVYENGTEVYVNGSASSPWSV
                     RDSNGKSVELPPYGWLVFNAKSKFYEMSANAGGRRIDYVTAPEYEYLDGRGQWSQQGN
                     LGSAGAVALRQKAEGVLELIDIYGNGRIGFRAAFDGVLMAYDPDGAPLGKVETSPREG
                     WCEFTPLAGARSYVFASAQ"
     gene            complement(52263..>52443)
                     /locus_tag="VLE22_06925"
     CDS             complement(52263..>52443)
                     /locus_tag="VLE22_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14175.1"
                     /translation="IGVLLLIYGVIILGFGLYGLTSPPEREVVMANLHTELWWGALLI
                     VLGAVYSYYFRPGRE"
BASE COUNT        10027 a        16407 c        16180 g         9829 t
ORIGIN      
        1 ctgcgatcgc gaaagtgcga gattgcgacg cggacgggac ttccccacgg atccttgagg
       61 cggtccagcc ttaagccatc ttctgacagc agacgcccga attcggcgac cgtagctgga
      121 tagtcggtct ggcggtcgag ctccagaaac gtcccgatcc cggagggcgc gaccgattga
      181 ctgtccagca gggcctcggc tacgagctgc aggtccgcat cgatgcgtgc cggatcgagg
      241 ttcagcaggt cagtgatccc gaacccgccg atcttcgact cgcgagattc gtaacccgaa
      301 tcgaaccagc cgtggcgacg gtttgcagtg tcgccagcaa cgcgttcgaa cacgctctca
      361 tctacgacgg caacgccgag ggcactccga gccggagatc cggaggatga ggccgtccgc
      421 accgtcaatg tggcttcatc gcccggccga tatgaacccg cgcctggcgc tgctatgatc
      481 tgcaggtcct ccgcgccggg gtaaagaatt gtgcgaccgg tctgatagtc gtcgcccagc
      541 ggcgaaagga tttcgatcgt cacttcgcgg ccgaaacgcg cgtcatacgg aatcaccgcc
      601 tcggcggagc ctttctttag gtgcaaaggc ttggagaaca gcacgtcgcc ccgtctgttc
      661 caagccagca gggtagcgtg cagatcatgt cgtggtgagg tgatgcggca acggacgggc
      721 tggccggcaa cgtacaagac ccgatcggtt tcgagccgga cctccagacc acccggccat
      781 acttgctccg tttgtgtggc ttggccctcg gaagcccggg ctcgcacggt gatctcgcct
      841 gaggtatttg gagggatact gagcctcgcc aggccgaacc ggttggtctt gcctgatccg
      901 agcacctgac catcaacgag agcctcaacg tcggaagctg ccggcttgcc atccggggtg
      961 tacgtcatta cgtacaatgg ccgccacaag gccgcacggt cgcgactgag cacgtaaata
     1021 tggatgggag cgcgcgagat ccgcacgtcg aacggccgct tctcggtgcg gttcgttccg
     1081 gcgtcagtca cgtaggctac atagtggaga tctgcgaagc gggcgtctcc aaggtcttca
     1141 cgcgcagccg caagatccag cgtggcgtgg aaagtgccac ctagtccaag agatccttcg
     1201 tgtagtgcgg tgtcgttttc tgccgcgcag atgcgcacgc tcccggcgct gagggacttt
     1261 ccaaacaggt agtccgcacg cacctctgcg atcgcatttt ggcccgagag gtagtaactt
     1321 ctgtccagcc gaactgtaac acggaaggat ggcagttcgt aacggcggat ctcgacgtag
     1381 cgctggtatg ttattccggc gtccttgtcc tcaagttcga tctcgtactt gccgttagca
     1441 gcattggccg gaacctccca atccgtgtgg gcgattccgt attgcgaagt cttcaggtcg
     1501 gccgagtgaa cagttgtgga gcgctcgtcg ctgatctcga ttccatactc cgctcccgag
     1561 cgcgccttgc cgtctgaccc cagggcgagt atacgaacgt gcacagtctg cccaggttga
     1621 tagagaggtt tgtccgtgca aatccggacg tcgttcggca ttacccccgg atgccacgcc
     1681 tcggtttcct gtacaagatc gccgagccgg ccgtggaacg tgatgttgtc tgggtattcc
     1741 caatcatccg tggccagcat cagccgcacc accgccgttc cgtccgcacc cgtcctggct
     1801 acaggcttat catcgagggt gatctgaacg tctgcgactg gcgccagcgt gattggatgt
     1861 gaggcgaaca cgtggaattc gtatggctgg ccgagcctag cgacaccgat gcccgcggcg
     1921 gttagtcgga acccatgttc ggcgatgata ggaaaactga tcatgccccg gatggggctg
     1981 aatgcggtga gattccttgt atcggcagaa acggaatagt gcagcctgaa gaaaaggggg
     2041 tcgtccgtcg gattgaccgg taaagccgcc tcggcaatgg acgaacccgg tttcgccgtg
     2101 aacgcgaact gtgtgcgacc gcggcgtgta ttttccgggt cgagccattc caattcgact
     2161 cgggtgtcga cgggtttttc gaactggttg tgaatcgcga gaaataccca ggtggaattg
     2221 ttaacaagct tggcgcgcgt ggcattctcg tccacggtga tctctgcgga agcagacgcg
     2281 cagaacacga gcgccaacgt agcagtaagc cgcatgggcg tagactccca gaataactga
     2341 attgtcccgg ctccatgctc aagtttaagg caaatctgtc cgaaaagccg gggccgcatt
     2401 gggaacgaac gtggtctcgc tggcgtatat ctcatgtggc aacgcgtcgg tgggtattcc
     2461 tgacaggaac gatagcggga gcggcgctcc tcgtattgac cgccgcctct gccgtgttct
     2521 gtgaaggcac cctccgcgtc cagaaaacgg cgtccgggag tggtgtgcct ccgaaggacc
     2581 gctatccaga tgccctgtgg cgaacggtgc atatccgggc gaaggacggc gtagcactgg
     2641 atggctggtt catcagaccg aacaagagta caagctctcg gtgcgtcgtc gtactacacg
     2701 gcatcgcaga ttcgaaatcg ggtgcggcgg ggttcgcgcc gatgtttttg gccgaaggct
     2761 attcggtcct gttgcctgac agccgggcgc acgggaacag cggtggggag ttcgtgacgt
     2821 atggcctgtt cgagaagagc gacgttctcg agtggacccg ttggctgcgg aaggaaggtt
     2881 gcagtgagat ttgtgggctg ggggagtcgc tcggcgcatc cgttctgatc caggcctcgg
     2941 cggaggagcc agtctttcga gcgattgttg cggaatgtcc gtacgctgat ctgaaagcca
     3001 tagccgtcta ccgcgtccag aaagtaatcc gagggccgca atggatctcc agcgccgccg
     3061 ccggtctcat tgttcacagc gggatgctgt acgccaagct caggtacggc ttagatttca
     3121 gccaggtggc gcccaccatg gcgattgctc gatcgaagac gcccattctc cttatacatg
     3181 gcgtgcagga cgggcggaca ccgtgttggc attcccagcg gttagcccag gcgaacccac
     3241 gcgctatcct gtggctggtg ccgaacgccg atcatgtcgg tgccagttcc gctgatcccg
     3301 acggtttccg atcgcgagtc ctggactggt ttgcgcgcag ttgacagagg gccgagcgaa
     3361 gaacctgcac ctcacaagcg cttggtggat ttcttcgata taaggtccta tgacgaatcg
     3421 ccgctcaatg ggttcgccct ggatacatct gacccctttg ccaattcggg ccgtggcttc
     3481 ggtggtagac tgcttgggtc atgacgactc tgacgctctt ggggattggt cttgtggccg
     3541 gggtatttgc cggcatgttc ggtatcgggg gaggcctcgt catcgtcccg gcgctgatct
     3601 ttctgttgaa gatcaaggaa ttggaagcga tcggcaccag cctggcagcg ctcataccgc
     3661 ccgtggggtt gctcggcgcc gccgagtatt accggaacgg tttcatcaac ctgcggtttg
     3721 cccttctggt cgcagccggc cttttcctcg gtgcctattt tggagccaag atcatgattt
     3781 cgcttccgcc caacatgatc cgcagggtct acggaacatt cctcatgctg atcgcgatac
     3841 gcatgcttgt tatgggtaag tagcgttacg tcaagagctt acgtgcgacg ccgtttttcg
     3901 gcggcaagct ctcgtggtgg aagaccgatg cgtcgctgag tcgagcatcc aaatcgccct
     3961 cctcctcgac cggcatctcg tgttccaccc agcgtgacag ggggatgttg cgaaagtaga
     4021 tgcgctcctg caatacgagg ctccgccgcc gctcctgcgc cgatttcgcc tctctcacgt
     4081 aatggcgctc ttccaccctg acgtcggaga ggaactcgtc aagggccttg cgccgcgcct
     4141 ccgcttcagc ggctttgacc cgttcctcca tgaccttgtg ctggaacgcg agggtcgccc
     4201 gtgcctcaac cagcgcctcc cgcctcttcg acaggtagac ctccatccgt gcctgggcga
     4261 tgatgaacga gatcactccg gagcccagag ctaccacaaa cggcaaagta atccagagaa
     4321 cttcgtgcat acacccgcct gccaagaaga tcggtgggga agtgtgctct tacgagggtc
     4381 tagggaagga ttccaggggc ggtaaggcga acgcctcagg gcaggaaagt ggccctctca
     4441 gaacgcactc tgcgagccgt cgatcttctg gctgctcaac aggcaactca acaaagagaa
     4501 gcagtagcgc gtgtcccgga acgtcaccag cgacgccatc gcagcgccta acgcggacaa
     4561 cgtcgccagg agccgggtct tcatcccggc cccgagcccg aagtgtttgc ccgcgtacgt
     4621 cacgataccc agcacacggt ccgcggcgaa ctgcgcgcga atcgccggat atgtcgctgc
     4681 ggcgggggcg gcttgcaaga ccgccgaaac tgtccgcaaa aggagcacgc gtttcgaagc
     4741 gcgccggatc tggcagcaca cttcgaggtc ccagcaggag ttgccgtacc gttcatcgat
     4801 gtaccgcatc cccttcagga agttcgcacg ggccatgagc acagccaaat tcaggaactc
     4861 gactggcaag gcgtccacat cgcacgccgg tgtagtccag tccgcaaagt ccccggcgcg
     4921 ccacgcgcgg tacgcctcgt ccggaagcgg aacccggcgg tgccgcgtca gtacgtcccc
     4981 ctcgggtgag agcagcaagg gagcgacagc agcagcttcg gccgtcgcct cgagctgggc
     5041 taccagcacg gccaccgtgt ccggttgaac ctcgacttcc ggtggcagga acaggtagta
     5101 gtcccccttc gccgtcctca tgccgatgtt cagcgccttc acaatcccga aattgcgcgg
     5161 aagacgcagc atggtgatct ttggaaactc ggcatccaga agaggagact cgtcatggga
     5221 accacagtcc accactacga cctccatgtt ttccctgccg gtggaccgct ccaacgcccc
     5281 gagacagcgc cggagagcgg cagcgttgtt ccaagacgct acgacagcgg agactttcgg
     5341 cggaggctga atttcttcca tgggaacaaa aatgataccg cagcgagctg gaagctggtc
     5401 tatttcgaca gggtatcgcc gcgcagcggg ccgtattcct ggagagtccg gtgacggatt
     5461 acttcattac ggcgaggatg ctgtctcctg gagcgaggac caccttgacg ggcttctggc
     5521 gcagcgtaaa cgagaaggac gtcggctcgg tgtcagtctg aagccagtgt gtctcaacct
     5581 tgcccttgcc gagatggatc tccagtggca catcgatgcc gaaatcagcc gcgacgtcgg
     5641 attgagtcaa cgtgccggcc acccgccatg cgggcgcttt cccgcgcacc gtgtgccgga
     5701 actgcagcgt cggaatgcct gtcgcgtaga cccagttgtc gaagaagctt tccagttgcc
     5761 ggtcggccgg tttcgggggc ataaactcgg ccgcgagcgc gcgaaactgc tccgtggtca
     5821 cagtacggaa ttcgtacctc cgccgcagtt ctccgagcat gccaagaaat ttctcatcgc
     5881 ccatccgccg ccggagcatg tgcatgatcc aggagccttt ttcgtacgtg atcgctcgcc
     5941 atgcccacgg cacgtcggga gtatccaggc ggacgcccca gatgatcggc ccgaccgact
     6001 cggtggtcct tccggacggc gacttcacca gcagacgccg ccggtactcc gtgagcaccg
     6061 attcgagcgc gtccggtccc ttccgtcgtt ccaggtacag caaagccgag tagttcgcca
     6121 gcgcttccat cagccagtca tcctgatagc ttcccgacgt cacgatgttg ccccaccact
     6181 ggtgggcggt ttcatgagcg tgcagtattt ccgagaagaa cagttgctgg taccctcccg
     6241 aagcggcggc cggacgctgc ttcgggtcga ggtacgaaat ggtggagaga tagacaagcc
     6301 cgggaaagcc ctggccgaaa gcgccgggga tcggcgagac cgtcaggttg cggatcggcg
     6361 gtggaccgaa gtgttcagcc atgaactcga acgcgtcggc gatctccgag gcgagtcctt
     6421 cgaggcgaag ggtaggatcc accgggagcg gttcgatgtc gagggtgacc agatcctcgg
     6481 gcccgggcct ccgccaggat ggagtgtgtt gcggcgccac cacctgccgg ggccgcggtt
     6541 gaagcgccgg ctccagcttt cggttggcaa acacctccac ggaatatggc ttgcggttca
     6601 ggctgacttt tcgatactcg ccgaggttga atccgaggaa gcggacgggc gaagacgtcc
     6661 ggcggcgtgt gatacgccac tctccctcgg tcctttgctc aacgacctcg ccccccgaca
     6721 ccaggtccag cgtggtggga tatcggaaca cggcgtcgca gtgggcgaac tgcgtcggcc
     6781 ggttgggata ccagttcccg cgcgagccaa catagtagac gccgttgccg gcctgtgcaa
     6841 ccacttcacc ttcgtgatgg aactcaatct cgtactggcg gccgggctcg agctcgcccg
     6901 gaggaatcag cagaaagaga ccgtcgcggc gcccgtggat caggttttcc cttagtgatt
     6961 cccgttggaa gacttcggcc ggcacgccat cgatcagggc ctgtgtaatc cgcatccggg
     7021 ccgaaagatc gaaggccagc gagcggtagc ccttctctgt cggggtgacc ttgaccttgg
     7081 tcactacctt gagcgagaga tccggttgga gtgtcgcatc gagccggaag tcgctcacaa
     7141 cgatgtcagg tggaggaaac tggcgcaggc ctttgcgaaa cggtcgtgac tcgaaactgg
     7201 tccatgtgtc gaagtgcgca cgctcgttcc ggaacgcgac cttgccgacg gtgatctgtt
     7261 cgcgggccag gggatcgtaa acgacatcga agttcccgag gcggccgccg ctgagggctg
     7321 catagaagaa gccgttggat ggaggccgag aagagagcag atccctgacc atccggatct
     7381 cgaagctgga agagaggttc cgcgccacca ccccccactc ctgttccatg agtacgcctc
     7441 gctccgggct cttgcggggt tcgcgctcct ggatcagttt cagcagcgcg tcgcaagtgt
     7501 cgtcggtgaa caccatcacc gccactttga agtgctcgtt gaggtttggg gcgtccgtga
     7561 gcgacgcaag cgcgagccgt tcgctccgtg tcggtggaaa gagcagtatc tccgcgtcgc
     7621 cggcttcgac gtcggcggtg aacaccgcgg aaacgcgacg ccctccgacc ggctttccga
     7681 agatcaggtg cccgtccgtg agatagaggc ggaggtcgtc cttggtgaag ttcacatcac
     7741 ggacgcgata gcattcgtcc cggtccaatc ccgcttcgcg aatctggttt gccaactcgg
     7801 ccgcggcacc cgcccaggca ggcaggctga atggcagtag cgccagcagt gggagaagac
     7861 gtttcatcca ctcccagtct actgtcttac gaggctggtt actcacgtcc agaaccggta
     7921 ccacttgcgg gcggaggggc tggcagcgtc gggctgctcg atgtggtctc ccgccagcac
     7981 ggcgccgggg ttccgcacac agagcaactc tgccgtatcc acaccgtaat ggctctcgag
     8041 gtagcgatag gcctcgtcaa gccggggtgg acgatgccgg ctgtcgtgag cgtcactggc
     8101 gacgatgtga accagcccgc ggcacatcag ttctccggcg aaccgcctgg cgctctttcc
     8161 aaagaggccg aagaacgatt gcgcagtgac ctgaatcgcg caacccactt gaacccagcg
     8221 ctcgagccgc tcgatccgtt gttgtagcag gcggttgcgt tcggggtgag tgattatggg
     8281 aacgaggccg gcgtcgagta agcggttgaa cacctcttcg gtggaccggg cgatcagaag
     8341 gtcggaaaac tcgacgagga tgtgcttctt ttgggcgatg gcgaattttg cgggattctc
     8401 gattgcttcc tggatgttct cgaagtgcaa gtgaaagtcg catccccggt gaatccgcac
     8461 gaccccatct gatgcggcag ccagttccgc gatcttccga tccacgagat cggggtcgaa
     8521 ctggaactcg aggttggagt gtggcgtcgc gacgatgtcc gtggtgcccg cctcggcggc
     8581 gacacgaagc atggcgaggc tctcttccat gtccctggcc ccgtcatcga gccctgggag
     8641 gatgtggctg tggatatcga tcatggaagg ctgggacgcg gggtgctacc ggccgggcgg
     8701 ttgcccggcc gcgcctcaga tgaacatctc ttcgtcgtgc gtagcctgtt cgacggtgag
     8761 gcggcggccc cggagcagcg cgccgacgaa cgctcggatg cccaccgcaa cgccgttgac
     8821 ttccttgatg ggtttgagca ctccgttgtg cacgagcgcc gctgtttcat gcaggcggct
     8881 aatggtgtcg tccagcacca actcgacacg gtccatctga atcttggcgc gtgtggtcgc
     8941 ttccgcgagg acgtcatcga tgcgcacaag ctgagtgcgg gccagatcca gtacctctcc
     9001 ggcctttgtc gtcacatccg tgagctgttt gcggctctgc tccagcgtcc gctgcgtcgt
     9061 ctgggccagg gactccacct gcccgcccac ttcacgaatc tgttcgctgg ccgtgcgagc
     9121 ggtccggtaa atagcgaaaa gcacaagagt ctgaaccacg acggcgagcg ccgacacgcc
     9181 aaccgccgcc gtcatcaaat agaacacagc atcctgattc atgaatagaa gtcggatatc
     9241 cttcttcggg cgctaaccct tcgccggcga agttccctcg gagccgggct ccggggccgt
     9301 gacagcctcg cggtacgcct gtttgccggc ctggacggcg gcggccaact gctcgcgctg
     9361 ccggccgatg gccgtcttgc ctttgtcgat caactctccg gcggaatcgt agagatcctc
     9421 gccccgcttg cgcaggtagt cttttccctc accggctctg ttccggatca gttcgcgggt
     9481 ttccagtccc gactttgggg cgaacagaac gccgaccgcc acaccgatgc cgagccctag
     9541 aaagaagtaa gaaagtctcc tatcgtcacc catgtcaccc tccagtcgcg gtgctggcta
     9601 agttgcctcg cttccattcg catactagca cattccatct tcggcggagc cggaatgccg
     9661 tgctagaatc gagtgggctt ccgggcgtat ttctctcaat cgcgctggaa gaaccgtgcg
     9721 gatggcggca cgcaaaccga aaaaattgga cagcgagggc ctctggagct atgcccttcg
     9781 ctcgctggcc gggcgggcac attccgtctc cgagcttcgg gagaagctcc gaagaagggc
     9841 tgagaagccc gaggacacgg ccgcaatcct tgcccggctg cgcgaacgcg gatacctcga
     9901 cgacaggcac ttcgcggaag gtttcgcctt ctcgcggctt ggcaaccagg gattcggcaa
     9961 gcaccgcgtg ctgcgcgatc tgcgccagcg aagagtagcg ccggcggtcg ccgaacgtgc
    10021 ggtccgcgag gcctaccaag acactgacga ggtggaattg atcgagagtt tcctcgcgcg
    10081 aaagtaccgg agagtgccgc tcgccgagtt tctcagcgag ccgaagaacc tcgcttcagc
    10141 ctaccgcagg cttcgcgtgg caggcttcag ctcgagtccg gtgatccggg tattgaagcg
    10201 gtacgcggcc gaggcggaat cgctggagac cttggacacc gaagacgacc ccggagaacg
    10261 gcgggacgcg gagtagtttc ggcgagctat tccacgagcg ccaccgggtt cctgagctct
    10321 ccgatttccg gtacgcggat gctgaccacg tcgtccggtt ggagggccgc ggactccgga
    10381 acgatgatgc cggtaccggt gaggagcact gatccggccg gcaccgggtt ggcccggagc
    10441 agatattcaa cgagcgtttc gaagcggcgg tgcagccgcg aggtggagac gctgcccgag
    10501 aagattgtgg aatcgccgcg ggtgatcgtg caggtcatct ccagttgata cggctccacc
    10561 aattcgtcga cggtcacaat caccggtccc aaggcgcagg aggaggtata catcttcgac
    10621 tggggcagat ataaaggatt ctctcgctcg atgtcccagg ctgaaacgtc gttagcaaga
    10681 gtgtagccga gaatccttcc tctccttccg agcacgagag ccagttctgg ttccggtgcg
    10741 gtgaacttcg agtcagcccg gataccaatg ggcttgcacg gccccacgca gactcttgcc
    10801 gtgcctttga agaagatctc cggcctcgat tcccggtaga catgcgcgta aatcccttct
    10861 tgggctccct gctcgccgtc gcggaaactg gcgctcactt cgtaggtgca gccgcaggcc
    10921 cacacttcaa gcgggagaag cggaatcacc gccgtgccct gctcggcgtg ccggctggcc
    10981 gactgcccgg ccagttcgct gagactgaca gactcagtct cggccttttg gatcaattcg
    11041 agaagagtgt acccgggaat gggtcgcacc atctctcgct cgaatacagc cgcggttatg
    11101 gtgctttgat gcctgatttg tccaagatgc atggaatgtt cagtatttca ggtaaacagt
    11161 cttaaagtcg gtataaaact ccagcgcggt cgggccctgt tctttcgggc cgaagctgga
    11221 gtctttcgtc ccgccaaagg gcaactggta ttctacaccg gcgctaggca ggttgacggt
    11281 gagcaatccc gcttcgctgt ggtatacgta atcgaacacc cgtgaaacgt tggtggtttg
    11341 cagcgacgcg gagagcccaa acggaatgtt gttggcgatc cggagggcat cctcgaaatc
    11401 cctggcgcgc atgacggcca gcaccggtcc aaagacctcc tccctcgcga tggtcatgtc
    11461 cgtggtgaca tcggcgaaga tcgtgggcgc cacgaagtag cccttgtcat aaccgtcgcc
    11521 cgtcagacgc tcgcccccga ttctgggcgg tccggcctct ttgcggccga tctcgatata
    11581 acggaggtcg gtgtcgagtt gagcctggtc gatcgccggt ccaatgtcca cgcccggttc
    11641 gagcccgttg ccaacgcgca gtttccgagt gcgctccacg agcgcctcga caaacctgtt
    11701 gtagattgac tcttcgacga tggcgcggct ggtagcagtg cacttctgcc cggtcgagaa
    11761 gaaggcgcca ttcacgacgt tctcaactgc gagcgcgaaa tccgcgtcgg cgagcacgat
    11821 cgtcgggttt ttcccgccca tttccagttg aatcttgagc cggcgtttgg acgcttcgcc
    11881 gtggagccag tgaccaactt cgcaagagcc ggtgaaagac accgccttga ccggtttcgc
    11941 attgatgagc gtcttcccga gttcgccgcc cgggccggcg acgaagttca caacaccctt
    12001 cggaacgccg gcctcgtggc acgcctcgac gatacgccac gcgctcagcg gcgcggccga
    12061 cgccggcttg atcacagcgg tattgccgca gatgagcgca ggcgcgagct tccacgctgg
    12121 aattgcactc ggaaagttcc acggtgtgat gagtccggcc acgccgaccg gcttccgcac
    12181 cgcgaacatg tgcacacggt ctcgctcgga gggcacgagg tagccgggca gccgcgatcc
    12241 ctccccggcg aaataccgga aaatgttgat ggcgcggcgg acctctcctt tggcttccgg
    12301 aagagtcttt ccctcctccc gggtcatctc ggcggcgagc tgttcgaact tccggtccag
    12361 gatttccgcg gttttgtaaa ggtagttccc tcgagccggc gctgggaggc tcgaccacgg
    12421 gccgagcgca gcctcggccg ctccggcggc cgcctcaacg tcagtcgccg aaccgcgaac
    12481 gaacagaccg accacttcgt cgacattggc tgggttgcga ttttcgaagg tggttccttc
    12541 gacccattcc ccgttgatgt aattgcggaa ggtttccata gaaatcactt atcgctcgag
    12601 cctctgaagc accgcacaca cagaaccgcg accgtaagcg agcggtccgg cgaacgtccg
    12661 gatggttccc caggcttcca gctcagcacc ctagaacgcc actttcggaa ccgtgcgcgc
    12721 ggcgggctcg gtcttaaagt atgcgtcgtt gcgcgcgaaa ccgaacagcg aggcggcgat
    12781 gttgttgggg aacagctcaa tggttgtgtt gtaccgctgg acggtttcgt tgtactttcg
    12841 ccgttcgacc gcaatccggt tttcggtccc ggcgagttcg tcctgcaggc gtaggaaatt
    12901 ctcgttagac ttcaggttcg gataattctc cactaccagg agcagccggc cgagggcgcc
    12961 gtcgaggcgt gtgttcgcgt cgatcttctc ttgcggtgtg cgcgcgccga gcagggcagc
    13021 gcgggcgctc gtgaccgact ccatcacttc ccgctcctgc tttgcgaacc ctttcacggt
    13081 ttccacgagg ttcgggatca ggtcggcgcg ccgctgcaat gcgacgtcca cctgggccca
    13141 cgccccgttg atcgactcgc gctgggttac caactcgttc cgaatcccga ccagcttgcc
    13201 tccgaagatc aggccggcga gcaacagcac gcccaatacg atcgccacag ctttcatatg
    13261 cacctcgtca tttctcgagt tgatccacgg cgtttattac cgtctgaact tgaaccagat
    13321 agctctcgaa gagcggagaa ggatccacgt cacgcggttt ccgcttaccc tcgcgcaaat
    13381 cgaggattgc ttcaaagggt gacgggtcga ttccgaaccg ttcccgggtc ttcgcgatga
    13441 cctgccgctt ctcaaagccg gattccgcac ccgccagcag caaagcgtgg cgaaagagaa
    13501 cgcaaaaagt ggacaacgag tcagacagca ggctccgcaa cagctccttg tcgccaagga
    13561 ccccggccgc cttctggcgc aaccgcagca gtttggcgcg caactcgtgt tcgacctccg
    13621 ccctgtagaa cgacttatcc acttcgaggt ctttgacgat atcctcgccg gacagaatgc
    13681 ggtgacgccc gcggatgtca tggaactcga tcggaaagca atcggtcgac gttcgcactt
    13741 cgtcaagact catcaggagc ggcgacgggt tcccaagttc gcgccaccag cggaagatcg
    13801 gagccgagtc cgccagttcg cgtgaggtca ccagccggag tacgcagaga atattgaggt
    13861 ccgagaaccg gccgtgatgg tcgcctaccg cggcagagcc atagagcacc accgacacca
    13921 ggcggtcgtc gtaggccttc cgcagtcgtt ccaccaactg attcagaacg cgttccatcc
    13981 gatcaccaga ttaccaactg ctggaggcgc cgcctccgcc tgaatctccg ccgccgaagc
    14041 cgccgaatgt gtcgccagag tcccatccgc caaaaccgcc gccactggaa cggccgccca
    14101 tggagcggcc cattatgtta cccgcgagca tggccaacag cagatcggcg cccgaaccgc
    14161 gcccgcggcg gcgtccgcca cccacaccat tcagcagcag caagagcatt accacaccgc
    14221 cgatgatcac cggccacggg atttcagagg cactcggacg cgtcctgcgc gggagcgtgg
    14281 tatcaagttt cacgcctttc gcctccgcga tccgtgagcc ggcaacctgg acggcttcgg
    14341 tcagggcctc tccgtaacgt ccttcgcgca aggccggtct catcgcccgc agaatgctgc
    14401 ccgagaagcc gtcagggatt atgggctcca gcccgtagcc gacttccatg cgcatcttgc
    14461 ggtcctggat caccagcagc agcaagaggc cctcgttctt ccctttctgg ccgatccccc
    14521 aacggcggaa catgtcgttg gctacgtctt cgatcggctc tccggcgagc gtggagagag
    14581 taaccaccgc cacctgcgcg ccggtctgcg tgtccagccg ggagcaatac cgttcgagat
    14641 tcgcccgtga gccgggatca atgacacgcg cgaagtcact gacgtacccc tcgggcttga
    14701 gggccgcaaa atcgacagga taggctgcgg cagcgcacac catcagggcg catgcagcga
    14761 gggagagccg gcgcattggt tgaagcctac agtgtacccc ggcccgccgg gcagtgggtg
    14821 cgcagcgcgc taacaggcca gttcttcccg cccgactcga agtccttcga ggttccccaa
    14881 caccgtcagg gcaatgtggc gagggtcgaa gatatcctgt gcgagctgct gcacttcggc
    14941 cgcggtaacc ttctcgatgc tgttcagcat ttcgtccatc gagatgaacc ggccgaaata
    15001 gagcgcctgg cgcgcgaggt tcgccatgcg gttggacgtc gattcgaggc cgagcatcat
    15061 cgagcccttg agatgatcct tcgcacggcg cagttcctcc tcgccgacca gttcctgctt
    15121 gagctgcttg aactcctcga tgatcaggtg gataacctgc cggcaggttt caagcgaggt
    15181 cccggcgtaa atggagagac accccgtgtc ccggtaggcg ctcagttcgg agaagacggc
    15241 ataggccaga ccgcgtttct cccggatgtt ttggaacagg cgggagctca tacctccccc
    15301 gagcaaagcg ttgagaacat aagagacgta tcgcccttcg tgcggcagcg ggtagcaggg
    15361 aacaccgaga cagacgtgca cctgttcgag cgacttcttg tttctcaggc tgatgcgcgc
    15421 gtgagtcgag gcgggtggtg tcgccggtac gctgccgttc ccggagagcg caccgaatcg
    15481 ttcgccgaca agtccggcca gttggctgtg cgtcaggttg cctgcggcgg tgatggtgat
    15541 gtttgagggg acatacacac tgcggtaata cgtctcgatc atgtccctgc caaagcgttt
    15601 gaccgtctcc cgcgtgccca ggattggttt gcccagtggg tgatccttcc agaagttgcc
    15661 cgaaaagacc tcgtggacga ggtattcggg attgtcggcc tccattttca gttcctcgag
    15721 gacgacgccc ttctcttttt cgacgtcttc atcccggaac accgggttca agacgagatc
    15781 gctgagcacg tcgaatacca gcggcaggtg ttcgtcgagg actttggcgt tgaagctgac
    15841 cagttccttg gcggtgaaag cgtcaagatt gcccccgacc gaatcgacgg aacgtgcgat
    15901 ttcctccgca gacctgttcg tcgtgccttt gaagaccatg tgctcgacga agtgcgagat
    15961 cccgttctcc tccgccgact cctgccggga accggtgccc atccagatgc caaccgccac
    16021 cgatcgcacg tggggtatgg tctccgagat gagccggatt ccgcttggga gctgggaaac
    16081 ctcgatatca cgcaccgata gattactact ggacatgagt gctgagagga ttgcgcctga
    16141 ttcattttcg cattccagac cccccttccg ctactcaccc gccgctccac agggcttgcg
    16201 aggaccgggt ggtagactgt tgtatatggc gcaagtcctt ctggggatgg aacttccgga
    16261 gatccaggcg ctgttggggc cgggccaccc gggataccgc gccaggcaga tttatgatgc
    16321 catctaccgg caaagggcca ccgggctttc ggccatttcc aacctgccgc gcgcgctgcg
    16381 gcatatgctt cagacggact ggcggatagg tttgccggcg ctcgagaccc gcttcgcttc
    16441 cactgacggt acgaaacgct accttctgaa gctcgacgac cagcgtacgg tcgaggcggt
    16501 gctcatgccg gagggagctc ggagcacgat ctgcatctcg acccaagtcg gctgtccggt
    16561 cgactgcaaa ttctgcatga ccgcgctgat ggggctcgag cgaaacctga ctgcgggcga
    16621 gatcgtcgga caggtcctgt tcgtggccgg caacaccgat ctttgggctg ggctggaccg
    16681 gctgaacatc gtgatgatgg ggatgggcga gcctctgctg aaccttccca acgtcctcaa
    16741 agccacccgt ctgctctgcg acccggacgg catcggcctg tccgtccgcc ggatcacgct
    16801 ctcgacgtcc ggaatcatcc ccaaaattga ggaactcggc agagccgatg tccgaccgaa
    16861 gctggccatc tcactcaacg cgtcgacgga ggaacaacgc cgggcactga tgccgatcac
    16921 ccggaagtac cacctgaagg acctgatgga tgcctgccgc gcctacccgt tgcgcccttg
    16981 ggagaggctg accttcgagt acgtgcttct cagaggagtc aacgacagcg acgcggacgc
    17041 taggcgggtg gtcggactgc tcgccaacct gaagtccaag gtgaacctga tcgcgctgaa
    17101 ccccggaccg ggcatcccgt tcgaaacgcc cgaaccagag cgcgtggagt ctttccaaac
    17161 gattgtaaag cggtccgtgc cgtgcttcgt ccgcaagccg cgcggccgcg acatcttcgc
    17221 ggcgtgcggt caactgaaga gagttgcgct cgcagagatc gcgtgaagac ctggacgcaa
    17281 tcctacttca ccggcatcgg gcgaggcgtg gcgtctcccg gaatcaatcc catcgtccac
    17341 ttgatcgctt ccagccacat tttctggaca tccgggcggt cccagacttc ctcgcggtgt
    17401 ccgagtccgc agtagaaaac tctgccctta ccgtaattcc gggcccaagt gaccgcgaaa
    17461 tccttgtcgg tgcgatgcac gccttttgtc ttcaggtcca ccttgtccgc atccaggcgc
    17521 atgagcacac gaacacgttc ccgggagaaa tcctttacct ggtaaatctc gtcgtgaatc
    17581 gtgaaggtgg gcgggaactg ttgcatgccg gggaagttgc gatcctcgac gatgatcggc
    17641 gcctggaact ggttccacgg gtggagatcg aaataaccgc cgatcatttc gccgtactcg
    17701 ggccatttgt agaaggtatc gttggcgctg tgcgctccga ggaacccctt gccgtcatcg
    17761 cggataaacg acaggaagtc ggccttctgc tgttcgttca tggcaagctc tccggtcgtg
    17821 tagaagaaga tcgcgtcgaa gaaatcgagg ttctttgcgt tgccgtcgag tttctttttc
    17881 gtgatgagct gcgtgtcggt gcgaatatag gtgtcgtaga gaccggactc ctgaccgatc
    17941 ttccagatgg ttccgagcgc gtggctgacg gagtcgtgtt gaaaaccttt cacctctcca
    18001 attgccagca gcttcttcct ttttgactgc gccggcaagg tcccggcgac cagcaggctg
    18061 cacaaaagga tgaacatgaa acgggcaagc ttcatgggcg tctccaatcc gcatcaatca
    18121 tcgcacacgc ccgatgcgga acgggcgccc gcggaccgcg gtcacaatcc gttgcggcgt
    18181 ctatcgccgc accatccgcc ggagcgtaaa gagttcggaa tcgctgttcg agttttcaag
    18241 gcgcttccaa gcgtctttga cgtggatgat cttgccgctg acgtggttgg aacggtcgga
    18301 ggccagaaag agagcgagtt gaatctgctg ctcgggtgag acgcccccgg tttcgcgcac
    18361 ctggcgtgcg tcctcgtagt ccttcgcccc ggcatcttct ccggcatgaa ggatctcgtc
    18421 cgtcatgtgg gtgtaggtgc cacccggcga catgcagttg atctgtacgt tgaagttgag
    18481 cacttcgccg gccaccgttt ccacgaaacg cgccaacgcc gtcttggatg cggcgtaggc
    18541 ggagaagttg gtgcgcggtc gagtggtccc tccgccggtg aggacgacga tctttccgcg
    18601 gcggcgctgg atcatctcgg gcaacaccgc acggcacgtg tgcatcacgc cgaaaaggtt
    18661 gatttcgatc gcctccgccc attgccgggg gtcggcggtg tgaaacggtc cgatcggacc
    18721 ctgcaccgca gccgccgcga ccaccaccgc gaccggtccg aactcctccc tgagcctctc
    18781 caccgcgccg cacacctgat cgaaatcgcg cacatcggcg ggcaggctga cagccgatcc
    18841 accagcttgt gcgatctcaa gcctcgccag gtctaactcg ggttggctcc ttgccaggag
    18901 gccgactcgc gcaccggccg ccgcgaaacc gatggccagc cgcttgccga tcccgcggcc
    18961 cgcgccggag atcagtaccg ttcttccgtt caccgacatc ccgggtcccc tcttcccaac
    19021 ccacaagggt agtacaaggc gggccgggtg acgaaaacac ggaatcgttt gcggaacgcc
    19081 aggagttacg acgcacactc ctctcccttg cacttgtaga gcaagacgtc cgctctggcg
    19141 aagagctcct cggcgacctc gcctggagcg tattccgccg cgccggccga gacccccaca
    19201 tcaatcacca gctcgcggcc cgcgcatttg accgaatacc gtccaccgag acgatcggcg
    19261 atctggcgcg tccgcgcctt cgcgtcgccg ctctcggagg aaaggaccgc cagaaactcg
    19321 tcgccgcccc aacggcagac caggtcttcc ggccggaact gctccaaaag gcggcgtgcg
    19381 aacgcacgca gcaactggtc gccggcgcta tgcccgtatc ggtcattcac cgacttgaat
    19441 cggtcgagat cgaaaagcat gatcgagaag cgccgctccg accgggccat ctcgccgata
    19501 atccgttccg cctcccggcg gttggcgaga ccagtgagcg ggtccgtaga agcgagtgtc
    19561 tccgccgctt cgagcctccg ctggaacatt tgcagttccg cccgcaactg ccgtactgtg
    19621 gcctggctct cggaccacat cacgtcaacc atcgcccaca gatgcgacag accgcccgga
    19681 acagaagtgg gttcggcagc cggcgccgcc tcgccggcca ccgccagatc cgcgaagatc
    19741 cgccgaagtt cctcctgctg ttgcccgtag aacgcgtcta ccttccggct gtactcgaga
    19801 aacgcccgct cgaaggcatt ccgcgactcc gcggatgacg ccgcttcgcc gtcgccgccg
    19861 tcacatcctc gaatggattc gatctggcga gcgtacgtgc tgaccagctc acgcccggcg
    19921 tccgaagcac ggcgggcgat ctcatccaat gaggaaacgt agcggctcag cgactcgtgg
    19981 aagagcctct cctgctgatt cggatcgttc gatggatcgt tgccggaggg catgtgggtc
    20041 gacacctgtc tccggaggat gatcggcgga ttgactgacg aaccgaatcg ggcgaaaacc
    20101 ttagatggtc ggggcgatca tgcgcgccag cgaatgcccg cgcccagcac cttctgcgca
    20161 tagttcacac ttcgctcgag gtccacccgc tgttgttctc gaagcgcggc ccggaactcg
    20221 ggcggtggat cgccgctgtc ggcgatgatc atcgtgccca tgaacgggtg tccattcttc
    20281 gccagtgcta caaagcgcgc gaattcggcc gccctcgcct tggggtaggc tttccagaaa
    20341 tccggctcaa ggtacggaag gatgcgtggc ggccggccgg tgatgatctc gaactgcatc
    20401 gcgaccttcg ggcaaagctc gcggaagcgg gcgacgaatg cgcggaagtc gagcgagccg
    20461 tcgccgagcg ccacccactg cgcggcggcg ccgcgcgggt gctcatagac gactgcgtcg
    20521 cggatatgcg tcgtaagcac gtagggggca agtacctcca gcgtaaccat cggatcttcc
    20581 accgcccata tcggatttcc agtatccagg catgcgccga catattccgg ccccgcctct
    20641 tcgatcagca tcttcagttc ccaggcctgc aagtcgccac tgtggttttc gagggcgatc
    20701 ttcacgccga ggtcgagagc ctgctggcgt accgcgcgca acactttcac cgtggactcg
    20761 atgtgcccct cgatcgggat ggagcccaga cggtcggcgc cgctgccgag atagcagcgc
    20821 atgacatgac cgccggcggc gcgggtcacg cgcaacccct ggagcgtgta ctcggccggg
    20881 tcgccgttct tcgcgctcca actggcggag gtcggacaga tgcacccggt gccgccctcg
    20941 acggcgatgc cgagccgttc ggcgtggctc ttgaccctgg cgagatgggc ggggtcgagg
    21001 ctctcgtagt tgccgacgct cgaaatctgg attgtgtcga gcttgagctt cgcggcgtag
    21061 tccagcaact cgacggcttt ccatccgaag gaacgcaagg aatacgtgtc gtagccaaga
    21121 gggattcccg ccgctggcgc gggcgcctcc gacttcccgg ccgcggcgct cgccgggatc
    21181 gctgagagcg tctgtagaaa ggttcttcgc tgcatggttg gttccccctg tccggttcgc
    21241 gacaattcgt ttttcggctg tccctccggc gcgctggcgg ccgcctccgg acatcgcgca
    21301 gaatcataca atacagcatg tcttccgccg aatccttcct tagcgagtgg atcgggcggt
    21361 ccggcccggt cacttttcac gaattcatgg aagccgcgct ctatcatccg gagttcggct
    21421 actaccggcg cgcgagggat ccgttcggac gggaaggcga cttctacaca gccgaacagc
    21481 tccagccggt tttcggaatt ctgattgccg cccgcatacg gtctctgttc gaggagatgg
    21541 gcagaccgcc ggacttccgc gtcgtcgagc tcggcgcggg acgcggggag atggccaagg
    21601 agctctcgtc attccactat gtttcggtgg agatcggcga cccgatgccg gagcggttta
    21661 ccggagtcgt cttctcgaac gagttcttcg atgctctgcc tgttcacgtc gccgtccgcc
    21721 gcggaacgag ctggaacgag atgagaatct cccggcggga ccagtgtttc gcttgggagg
    21781 agtgtggcga ggcgccggaa gaggttcgcc ggtatctcga ccgctacgcc ggttcgccgg
    21841 aggaagggaa tctgtgcgag gccaacctcg atgcgctgcg gtggctggag gagatcgccg
    21901 cccgcctcga gcgtggatat gtcttcacga tcgactacgg atacaccgcg cgggagctga
    21961 tccggtttcc acatggaacg ctgatgagct atcaccgtca caccgccgcc gagaacgtgc
    22021 tcacaggcgc cggcgagcgg gacatcaccg cgcacgtcaa cttcaccgcc cttcgggacc
    22081 atggcgagcg aagtagcctc gagacaatac gcttcgaatc gcttgcccgc acgcttctgg
    22141 atgccggtga ggccgatcag ttcgcggcgg cgctgtcgag cggcggggaa gcggagcgga
    22201 tgcgccggac gctgcaactg aagacgctgc tttacggaat gggggaaacg ttccggacgc
    22261 tgcttcagaa gaaggccgca aaataaaaac ggccccggaa acccggggcc gattcactga
    22321 acttttgccg cagccccttg tcggggcgcc gctgagctac ttctttttct tcggagcagc
    22381 cttcttcttc gcgggctttg cagctttctt cgttgcgttc ttcatcgatt gattctccca
    22441 aacatctaaa gttgcgcgat ctcctaaaag accgcagcgt gattcatata taaagtggat
    22501 tgagcagaaa gtcaagaaaa aaatgcacgc tgactcatga aaggtgattc gtcatgatcg
    22561 gaggctttga cgaaaaaaac gttccacagc aaaattgttt taatagaaac atggtgtgtc
    22621 cgcggatttt cgggttccgg cgttcactgt cgccggcgat cgtcgcggtc atcgtcgccg
    22681 cgctggtatt gctcaccgcg tgcgctcgca agaggccggc gcgtcctccg atcgctcctg
    22741 ttcccggtgc gcttgagttt ggcgtcgcga gttggtacgg ccatccgtac cacggacgtc
    22801 gtgcggccaa cggcgaagtg tacgacatgg agaaactgac cgccgcgcac cgcacgcttc
    22861 cgttcgactg ctgggtggaa gttctcaatc tgagaaacaa caagtcggtc caggtgcgga
    22921 tcacggatcg aggaccgttc gtcgacggcc ggatcatcga tctctcccgc gccgcggcgc
    22981 ggcagatcga gatgatcggc ccaggtagcg ctgaagtacg gttgcaggtg attgcgcctc
    23041 cgcaggcgcc ggtggcggag aacgtctttg cggtgcaggt cggcgccttt caggatcgag
    23101 gcaacgccga gcggctggcc ggagaaatga agcagcgctt cggagagagc cggctggtgc
    23161 agcgcgacgg ctcgccgcgg ttgtggcgtg tgctggtggg ccgggaaccg aatctggaag
    23221 acgcgaatgt tcttgcggag cggatccgcg ccgaactggg cgaagcattt gtcgtgcgct
    23281 tggactcccc ggccggtgat cgcctataat cggaaacaag aatgttcgcc gtccgtgcgg
    23341 actccccgcc cgtcctcgtg ggccagtcct tccggatccg ccaggtcctt caactcatcc
    23401 agaaactcgg acgctccagg tcccccgtgc tcgtgttggg cgaaaccggc acaggcaagg
    23461 aagtcgtggc ccgtgcgatc caccacgttg ttccagcagg cccgtttgtt ccgatcgact
    23521 gttcggctct cgtcggtcca ttgatggaga gcgagttgtt cggtcacaca aagggtgctt
    23581 tcaccggcgc cgccaccgcg aaaatcggac tcgtcgaagc cgccaacgga ggcaccgcgt
    23641 tcttcgacga gatcggcgaa ctgccgctcg atctccaggc gaagctgttg cgtctgctgc
    23701 aggagaaaga gtttcgtccg gtcggatcgg tggagcggcg caaagcggac ttccgggtga
    23761 ttgccgccac caaccgcgat ctcgtccagg aagtggagcg cggccggttc cggcgtgacc
    23821 tctactaccg tctcaacgtg atcacgctcc gggtgcctcc actccgcgaa cggaaggagg
    23881 acatcccggc tctggtgggc tacttccttc gactccatgg cggcaactgt tcggtgtccc
    23941 gcgaatgcct cgatgcgatg atcgcctacg actggcccgg caacgtgcgg gaattggaaa
    24001 attgtgttca gcggatgtcc gccgtcaact ccggcccgct gctgcagacc gccgatcttc
    24061 cgacggccct gcatacctac atggaggcgc gaagagccgg gagcctggtg atgaccgccg
    24121 ttgccgttgc cgacgtatcc gtggcgcccg ctccgccggt tctctcgctc agcgaagtgg
    24181 agaagcgcac gattcgcgag gctctggatt acacgaaagg tgatagagta atggcggcgc
    24241 gcctgctggg catcggcagg accacgctgt atcgcaagat caaggaatac cggcttaccg
    24301 attgagaatt gctttagacg ccacttacag cgtcggagat caactgtccg gcgtgggagt
    24361 atacagccgc gagatgctgc tcggactcgc gcaggcccat ccggagagct gcttcctgtt
    24421 ctgttttcgc ccgcaccgct ttctgcgcag ccttcgactt cccgtgcccc gcaacgcatc
    24481 ccgccggatt ctgatcgggg aacgcccgcc ccggctggcg gatctctttc acggcctgaa
    24541 ccagcggctc ccgcacggac gccaccgccg tgccgtctcc acatttcatg atctgtttgt
    24601 cctgactggg gagtactcga cggccgagtt ccggcggcgc tttgccgcgc aggcgcgtga
    24661 tgctgccgaa cggtcagacc tcatcatcac cgtctcggcg tttacagcag gccaggtgga
    24721 gcagttgctc ggcgtcgcga gggagagcat tcgcgtgatc ccgcacggcg tccggcttcc
    24781 agccggcgat cgagtggctc gggagaagat catccttcac gtcggcgcga ttcagaagcg
    24841 caagaacatc acgcgcctcg tggaggcttt cgaaaacact ggaccggatt ggcggctggt
    24901 gctggccggg tcggcgggct acggcgccga agacgtcctc agccgcattg ccgcgagccg
    24961 ccgccgtacg gatgtcaagg ttctcggata ccttcccagc gccgggctgg cggatttgta
    25021 ccgccgagcc tcggtgttcg ccttcccgtc tctcgacgag gggttcggga tgccagtgct
    25081 cgaggcgatg gcgtcgggag tgccggtgct gacgtccaac cggtcggcgc tgccggaagt
    25141 tgcgggcgat gcagcgttgc tggtggatcc cctcgacagc caggcgatcg ggaacgcgct
    25201 tcggcgactc acggtagatg atcaattgcg ccgcgagctg atcgagcgcg gactacgccg
    25261 cgccgttggt tttacctggg aagaatcggt gcggaagact tgggcggtgt acgaagaact
    25321 gctgggctga gcgctagtgt gcgtcttcgt cgacggaaat cttgagggcg cgaaggaact
    25381 tctgatcctg caaggtccac ttgaccttgc ccttggcttc gaattcgccc gccggcgcct
    25441 cgaaagtctc atcgatggtc gccgattctc cttcgacggt gtcttctttc ttgttgaacc
    25501 cgatggtcgc ttccaggacc aggaagacct cgtagcccgc ccgcttaatt tcgccaatgg
    25561 cggctgcgat ccgatcggat tcggaaagcg actcgttaat cgcgttcccg agttcttgca
    25621 ttaagcgctt cagtcgctct tccaagactg ccctccgacc tcctccagaa taccactctt
    25681 cttcccttct atcgtccgaa ccggcacagt cccggagatc gtggatgatg tcacggaaag
    25741 agtgcgtctg ctgcctttgc taccatgaga gggaaaaacc catggcaaat atcggtcgcg
    25801 agttcgtgcg ccacgtcctg cccaagatcc tcaagccgat gcgggcctta tggaacgaaa
    25861 tgatcggctt cgtcttcctg gcgctggcgg cgattccggt cccgcgcacc ttccgcatgt
    25921 ggcaggaata tgacgaggca ggcgagggcc tgttcaagat cgtcctctcg ggaatcttca
    25981 tcgccatcat ggcggtcttc ggcgttcatt ccttcctccg cgctcgcaga atcacccgtt
    26041 catgagcgat ccgcgcggaa agcccggcca gtttccttac acgcgtggca tttatcccga
    26101 catgtaccgc ggccgtctct ggacgatgcg gcaatacgcc ggattcggag cggcggagga
    26161 aagcaacgag cggtaccgtt acctgatctc tcaaggcacg acgggcctgt cagtcgcctt
    26221 tgatctgccc acgcagatgg gcatggactc ggatcacccc atggcggcgg gcgaggttgg
    26281 gcgcgtcggc gttgccattt gctcgttggc ggacatggag aggctgttcg agggcatccg
    26341 gctcgatgca atcaccacgt cgatgacgat caactcgacg gcggccaccc tgctcgcgtt
    26401 ctatgtcctg gtggctaaga gacagggcgt ggacgtacgg cggctctccg gcaccgtcca
    26461 aaacgacatt ctgaaggagt acgttgcccg cggcacatac atctacccac cacgtccggc
    26521 aatgcgcatc atcaccgacc tgttcttctg ggcgtcgagc gagctgcccg agtggaacac
    26581 gatctcgatc agcgggtatc acatccgtga agccggctcg accgctgtcc aggaagtcgc
    26641 tttcaccctg tccaacgcca tcgcatacat tcaagcggcg ctcgacgccg gacttgcgat
    26701 cgacaccttc gcgccgcggc tctcgttctt cttcaacgct cacagcaact ttctcgagga
    26761 gatcgcgaag ttccgggcgg cgcgcaggct ctatgcccgt atcatgcggg accgcttcgg
    26821 ggcgaagagg ccgcgctcca tgacgctccg cttccacgcc cagaccggcg gctcgacgct
    26881 caccgcgcaa cagccggatg taaacatcgt tcgggtctcg ctccaggcgc tggcgtctgt
    26941 gctcggagga gcacagtccc tccacaccaa tgcccgggat gaggcgctgt cccttcctac
    27001 cgaggaatcg gtccgtctgg cgctgcgcac gcaacagatc atcgcgcatg aaagcggcgt
    27061 gacgcagacc gcggacccga ccggcggcag cgcgtttatc gaggaactca cagaccgtat
    27121 cgagcgggaa gcgcaactct acatcgcgag aatcgacgag atgggcggca ctcttcgggc
    27181 catcgagtcc ggctacattc agtcggagat tcagaacgca gcctacgagc atcagcgcgc
    27241 catcgagagc ggagagttgg tggtcgtggg ggtcaaccgg ttccagatgg agaaacgcgc
    27301 gatcccgatc ttccggctcg atccggagct ggagaagcgt cagatacaac gtcttggaga
    27361 attgcgagcc ggacgcgaca aggggaagct ggaggggagc cttgatcggc tcgaagcagc
    27421 cgcccgcgga gaggagaacc tgatgccgtt catcctcgat gccgccgcgg cgtacgccac
    27481 ggtgggcgaa atctccaacc gtctgcgcaa agtcttcggc gaataccgcg aaaccggagc
    27541 aagcacttga ggaaccgcgg ccctgaggga gcggcgttta cagcggcagg atctcctccc
    27601 gatccgggtt gaacttgatg cggcgctttt gaacgtacgc gatgttacca aggtgcgacg
    27661 cctgggccga gcggtggccg atgtacacgt ccccattcgg gagcttccgc gtgcggcagc
    27721 attccagaaa attctccacg tggtcgatgg tctggtcccg ctcggacttc accaccaccg
    27781 gctgtgcacc cttcccaacc ggcgtgaact cgaaccggct acgcgtaatg aaaaggcgcc
    27841 cttccgtgcc gcacatctcg acgcccgcgc ccgtgatgcc gggcgccagg gttgcttcga
    27901 acgtagccgt ccactccgcc ggatactcga gcagcacgtt gatcgtgtct ggagcggtgc
    27961 ggccgtcctt gtagtggtag actccgccgg aggcgaccgc cgccgtcggg ttgtcctggt
    28021 ccatgaacat gtggactacg tcaatccagt gcgtgaacag gtcggtgacc tggccgccac
    28081 cgaaatccag gtacgcccgg aaattgaaat actgcggcgg atcccactcc cgccatttca
    28141 ccggaccgag ataccgcgcc cagtcgagat tggacggttt ttccgccatg ggcgccttca
    28201 tcagatgcgc gccgttaccg tgccaccacg tccgcgccag cgtgatcttg cccagcttcc
    28261 cggctttgat gtactcttcc ttcgcctgga tgtagtgcgg gcctgagcgc tgctgcatcc
    28321 cgacctggca gatgcggttg ttcactcgcg ccgccttgac gatctccggg ccttcctcga
    28381 ttttcagcgt caggggcttc tcgacgtaga cgtccttgcc ggcgttgagc gcgtcgatcg
    28441 ccgtcaatgc gtgccagtgg tccggtgtgg cgatgaggac cgcgtcgagc tctttcatct
    28501 ccaggagctt ccggtggtcg cggtgactcg tcgcaccggc cgccttctga agcgcgcggt
    28561 cgatcctgtc tccgtagatg tcgcacacgg cgtccacctg gacggagccg gtctcgatga
    28621 atgtgcccat gacactgcgg ccccggccgc cgcagccgac cagaccgaga tgaacgcggt
    28681 cgttggcgcc gagcactcgc gaataggaag aagccgtcat cgccagagcg ctgccggcga
    28741 accgaaaaag atcccttcgt ccaaagtccg cgtggaacat aagggtgcaa ttatatcgct
    28801 cgtgaacagc gccggataga atgagagcag gcacttgacg cgttccatcc acattctcgg
    28861 cggcgggctc gcgggcaccg aggccgctct tcaggcgtcg aagctgggcg ttccagtggt
    28921 tctgcacgag atgcggccgg cggtgccgac ggctgcgcac cggacggacc gcctcggcga
    28981 attggtctgc agcaactcgc tcaagagtga acaggagtcg accgcgcctt ggttgctgaa
    29041 gcaggaactc cggcgcctcg gttccgttct gctggaagcg gcggcgaaag cgcgcgttcc
    29101 cggcggccac gcgttgaccg tggacaggga ggtgtttgcc gaggaggtta cgcaggccgt
    29161 cgcatcagat ccccgcatcg aactgcgccg cgacgaggtg cgcgagatcc ctcgcggcga
    29221 gatcgtcatc gtcgccaccg gaccgctcac cagcgatgcg ctggcgtccg atatcgcggt
    29281 tcttaccggc tcggagcggc tttacttcta cgacagcatc agcccgattg tcgaagccga
    29341 ctcgatcgac atgtcggtcg cgtttcgcgc ctcgcgctat gacaagtcgc tcgactcctc
    29401 cggggactac atcaactgcc cattcgatag agagcagtac gagcgcttca ttgacgcact
    29461 gctggaagct cagcaacacc agccgcacat ctccgccgac gtcccatact tcgaagcctg
    29521 cctgcccgtc gaggagctcg cgcgccgcgg gcgcgatacg ctgcgcttcg gcccgatgaa
    29581 gccgatgggc ctcaccgacc cccgcaccgg gaaccggccg tacgcggcgg tccagttacg
    29641 gcaggaaaac ctccgcgccg acagctttaa catggtcggc tttcaaaact acctgcgcta
    29701 cggtgaacag gcgcgcatct ttcggatgat ccccggcctc gaatgcgcgg agttcttgcg
    29761 ctacggccag attcaccgga acacgtacat caacgggccc gcgctcctcg atgcccttct
    29821 gcggctgaaa gcccaccccg gaattttctt cgccgggcag gtttccggtg tggagggtta
    29881 cgtcgagtcg attgcgactg gtctggtagc cggccgcaac gccgcttccc tggcgctcgg
    29941 cgaagagccg cgcccgctgc cccgccagac cgcccttgga tcgctctgcg cctacgtctc
    30001 cggggcggat ctggacaatt accagccggc gaacatcacc ttcgacctgc taccgccgct
    30061 cgacgacgcg acgaagcgga agctgcgtca cgacaagcgg gcccgccgcg tcgaggtatg
    30121 ccggagagcg cttgaggcac tcgaggagca cctgggagtc catgtctgag ctcgcccaac
    30181 agatccagcg cttcctcgat tctcgccgcc gggagaacgt ctccgagcac acgctccgcg
    30241 cctatggggc cgacctgaaa cagtttctcg aatacttctc gcctcagggt gcggagcctc
    30301 cggcactcgc cggcctggat ctgttgctgc tgcgggaatg gatggctgac ttgtacgccc
    30361 gaaagctcag ccccgtcact ctccggcgga aactcgccgc cgtgcggtcg ttcttccatt
    30421 tccttcaccg cgaggggttg atcgggatga acaccgctcg tctgctcggc acaccgaaaa
    30481 ctccgaagac cctgccgcga gttccctccg ccgagcagac aaaccgcatc ctcgatgggg
    30541 ctggcggcca aaccgaacgc cccagcctgg cacgcgacct ggcgatcctg gaaatgctgt
    30601 acggctgcgg cgtccgggtg tccgagttgg ccggcatgag cctcgacgac attgacttcg
    30661 gcgagcagtg ggtacgagtc cggggcaagg gacggaagga acggcaggtc ccgatcccgt
    30721 ctaaggcagc ggccgctgtc gaacgctacc tcgcggagag agtcgcgccg cccggcgaac
    30781 gtgccgtctt cgtgaaccgg cggggcggcc gcctgacgga ccgcgctgtc cgggccatcg
    30841 tcaagttcta cgcgaccctc gtcgccggcg attcgtcaat acaccctcat acgttccggc
    30901 atgcgtatgc cacgcacctg ctcagcgacg gagcggacct ccgggcgatt caggaactgc
    30961 tcggacacac ccggctttcc accacgcaga agtacacgca ggtgtcgctc accgagttaa
    31021 tggcggttta cgatcgctcg catccgaagg cgtaggcggg aacaggccgc cttgctcgag
    31081 caaccgccga ccttcctccg aaaccaggaa atcgaggaag cgtttcgcgg catccggctg
    31141 cgctgtggcg gccacaattc cacccgcctg ccggatcggc tggtgccagc tctccggaag
    31201 caagatgccc tccttattat gcagcaacgt ccaggacgtg atcgtagcgt cagcgttccc
    31261 actggcggca tactcgaagg cttggcggac attctcgccg taaacgagct tcccacggat
    31321 ggcctcaaat aacccacttt ccttcaataa gttgagggca gccattccat acggggcgtg
    31381 agtcgggttg gcgatcgcca catgaagcac ttccggtttg gtgagatcct gaagcgtccg
    31441 gacggagccg ctgcgggacc agagtcccag cctgccatgg gcatagatcc gaaccgagtc
    31501 ctgccggatg tgtcccgagc ggacaagatc gtccacaaac ttttgattcg ctgagagata
    31561 gacgtcgtac ggcgcaccgt gttcaatctg tcgggcgagc atcccgctcg atgcgagagt
    31621 aaacgtcacc cgaattctcg tggcacgctc gaaagcggct ttaaggctgt ttcccagcgg
    31681 tgccaggtcc gccgcgcctg caaccgtgac cccggctgtc aaagactgac cgagaagcaa
    31741 gaggagcgtt agccacaata caatgaaaga tgataagcgc cttgacaatg gcgcactaag
    31801 gtttgataat gggaatgtgc taacgcatgc cgggtctgat cggtatggca tgccgaaatg
    31861 cttcatactg ttcaaagtgt agcgtgtttc gctggaggat ttaagaaata tgagtaagat
    31921 catcggaatc gatctgggta ccacgaactc cgtggtggcg gtcatggagg gcgggcagcc
    31981 ggtggtcatt cccaaccaag agggggggcg gacgacgcct tctgtggtcg gttttgccaa
    32041 gagcggcgag cgtctcgtcg gccaggtggc taagcgccag gcggtcacca acccggagaa
    32101 caccgtttat tcgatcaagc ggttcatggg gcgccgcgtt ggcgaagtca ccgaagagat
    32161 gacgatggtg ccctacaagg tggttgccgg tgaaaacagc gatgctcgga tcgagatcca
    32221 gagccgcaga tattcgccgc ccgagatttc ggccatgatc ctgacgaagc tcaaggaagc
    32281 ggccgaagcg tatcttgggg agaaagtcac caaagccgtc atcaccgttc ccgcgtattt
    32341 caacgactcc caacgccagg cgaccaagga cgccggcaaa atcgccggcc tcgaagtgat
    32401 gcgcatcatc aacgagccca ccgcggccgc gctggcctac ggtctcgaca agaagaagga
    32461 cgagacgatc gccgtctacg atttcggcgg tggcacgttc gatatctcga tcctcgaggt
    32521 cggcgacggc gtcgtcgagg tgaagtccac caacggcgac acccacctcg gaggcgacaa
    32581 tatcgaccag cggatcatcg agtggattgt cggtgagttc aagcgcgaga acgggatcga
    32641 cctgtcaaag gaccagatgg cgcttcagcg gctcaaggag gccgccgaaa aggcaaagat
    32701 cgagctttcg acgctgctcg agaccgaact gaaccttccc tttatcaccg cagatgcgac
    32761 cggacccaag cacctggtga tgaaactcac cagggctcgc ttcgagcaga tggtcgagga
    32821 ccttctgaac cggagtgttg gcccctgcaa gcaggcgctc gccgacgccg gtatgacccc
    32881 ggccgacatc gatgaagtcg tcctggtggg tgggaccacc cgtaccccga aggtgcagca
    32941 gattgtacgt gagctgttcg gaaaggatcc gcataaaggt gtgaacccgg acgaagtggt
    33001 ggccgtaggc gccgcggtcc agggaggggt gctcggcggc gaagtgaaag acgttctgct
    33061 gctcgacgtc accccgcttt cgctcggcat cgagacgctg ggcggtgttt tcaccaagct
    33121 catcgaccgc aacaccacca ttcccacccg gaagagcgag accttctcga cggccgccga
    33181 cagccagacc tccgtggaga tcaaggttca ccagggggag cgcgcgatgg ctgcggacaa
    33241 ccgcctgctc ggagtcttcc agttggttgg catcccgccc gcgccgcgcg gaatcccgca
    33301 gatcgaagtc acctttgaca tcgacgccaa tgggattctg aatgtgaccg ccaaggaccg
    33361 cgccaccggg aacgagcaga aaatcaccat cacctcctcc tcgggtctcg gcaaggaaga
    33421 ggtcgacaaa atggctcgag acgcagagtc ccacgcggcc gatgaccgca agcgcaagga
    33481 cgaggtcgag gctcggaacc gcgcggactc gatggtctac cagaccgaaa agctgctcaa
    33541 ggagcatcgc agcaagatca gcgacgccga cgcacgagac attgagtcgg ctcttgagga
    33601 aaccaagcgg gccgtccgcg aggggacggt cgagctgatc aacagtgcca ccgatcgtct
    33661 gaccacggcg agccacaaac tcgccgaggc catgtaccgg gcgacggcgg agcaggcaag
    33721 cagtgcggga cccgcgcagg agcagccggc ggagccgccg aaaggaaaag agaacgtcgt
    33781 tgacgctgaa tttgtcgacg tggacgacaa gaagaaataa accgttgccc gcggcccgtg
    33841 ctgtccgata tcgcacgggc ccagggctgg aacgcgtgtt tgcatggcga gtccggacta
    33901 ttacgagacg ctaggcgtgg cgcgcggcgc caagcccaat gacatccgca aggcctatcg
    33961 acgcctcgca aggaaatttc accccgatct caaccccggt gacaagtccg ccgaggagcg
    34021 tttcaaaaag atccaggaag cttacgacat cctgggcgat ccaaagaaga agcagatgta
    34081 cgaccagttc ggcttctact ccgcctcggg tgcgccgggg gggcaggagg gattccgggc
    34141 aggtccggag atgggtttcg gcggatttga tttttccgat ctgttcagcg gcgccggttc
    34201 ccgagccaca ggtggatcca gcaacgcctt ccgcgacatg ttcagccagt tcttcagccg
    34261 cggtgcgcac ggcacggagg ctcgtgggcc ggagcgtgga tccgacctcg agtacggtct
    34321 gagcgtcgac ttctggcagg ccattcgtgg tacccaagtg cgtctggaca tcaaccgcca
    34381 ggaagtctgt gacgtgtgcg gaggctccgg aaagcatgga ggccagtccg ccgtctgccc
    34441 ggagtgcaac ggcagcggca ccgtgactca aatcgccggc gcgatgcgct tcagcctgag
    34501 ctgcccgcgt tgcggcggac aggggagact tcgagactcc tgcgagaagt gccatggtga
    34561 tggccggctc tccaaggccg aaacggtgga agtcagaatc cctcagggcg tcaagagcgg
    34621 tgaccgtctc cgcgtgccgg gcaaagggaa ctccggagcc gggggcggcg cggctggcga
    34681 cctgtacatt acagtgcggg tggagccaca cccctttttc cagcgggagg gcgacgacat
    34741 cgagatccgc gtcccggtca cggtgagcga gaccggcctc ggggccaaga tcgaagtgcc
    34801 gaccattgat ggacgtgccc tgctcaaggt cccccagggt actcacagcg gacagagatt
    34861 ccggctgcgc gaaaaaggag tgttcaacgc ccgcaagggc gtccgcggcg atcagattgt
    34921 cgaggtcctc gtccagtcgc cgaaggtgcg cgatgaacgg accaaggaga ttctgcgtga
    34981 gctggccacg ctgaacccgg aagatccgcg cgctgagctg tggtcaaagg tgtaaccgat
    35041 gccgaaacga agatcgaaag ccgcatacat gatctccgct gtggccgagc agtaccagct
    35101 ccatccgcag accctgcgcc tgtacgagcg cgaggggctg ctgaagccgt cccgcagcga
    35161 gggcaacaca cgcctgtata cggacgacga cctggagcgc ctggaggtga tccttcagtt
    35221 gacgcgcgag ctcggcgtca atctagcggg cgtcgagatc atcttgaaca tgcgcgagaa
    35281 gatgtcggcg atgcaacggc agattgagga attcgtcgcg acgctgaacc gggagatggc
    35341 tacgcacttc cactcgcccg agccggcgga gcatacttcc ttgatccctg tgattcactt
    35401 agccccgctc gttgtgcaga atgcaccccg gcggcgcaag cggcggtccc gcgacgagga
    35461 ctccgccgcc gttgccggtt aagcttgacg cctaccgcat cgtctgaagg tattctccgg
    35521 tgaccgcgtc gacaaagatg gaaaagttgc tgctggcgac cgaatcaccc cagatcacgc
    35581 gccatgccgg atcggggaat cggggtgtcc attcgagaat gaacgagatc ggcatctcgg
    35641 gattcttctt cgcgtagtcg gcgcctttct ttagcgcgat ctcgtaggct ttgtccgtat
    35701 cagtctttag ggcagccacc agaaacggtt tggcctgtcc ccggggtccc gaccatcctt
    35761 cctccagccc ggcgaaaaca cccttgtgga gatttcccgc cgactccacg accgagtagg
    35821 tgtagcttcg agcgcgtccg cggctctggg acacgaaagt cgcttcccat gcgccggacg
    35881 cccccgcggc gcttttcact tccgagatcg gtatgctacg cacccgcagc acctggacgt
    35941 ccggagccca ctgccgcgcc ataccgtaca tctgataaaa ggccttctgg ccggtgatag
    36001 gatccgcggg cttctcgggc tccttcgcct tctgtacgtc aggcttcggc gccggcgagc
    36061 agcctgccag caaaagaact agcgcgagaa gaaaagggac tttggaatgc catttcatgc
    36121 tgaaatccta cctcgtaccg gaagtagcag caactcagga tgttacgctc cggcggctac
    36181 gagcctttgc cgtcatcctt ccaccacgcc tcgatggtgg ctcgataacg cgagtacaga
    36241 taagacgtaa gcagcaacag gacaccgagc accgcgaacg ccgtcacccg gtagatacgg
    36301 cggagcagcc acacatcgta cagatacagc ttgaggacca cggccgccag caagccgagg
    36361 ccgagaaggc ggttgatcct ggacgtcgcc agcacccccg ccgtcaccag ggccagcgca
    36421 taaaacgcca tcaggatcga gagcgaagtg ctccgaaggc tcggaagatt gtcagccgtc
    36481 gcactgcgcg cggcccaacc gaaaacttcc agcgcgaatg cccagaggac cacggcgtgc
    36541 gcggcgacgt acgatgctag cacgtaaggc ccggaccgcg tccaacgcgc tgcgaggaaa
    36601 agagacgccg ccgcggttgc gaaggtaaga aagcgcagat tcgcgaccgc atggtacgcc
    36661 gccgggtcgc gatagaggcc ggcatcgacg aacagaagcc gcgcggccgt aagtgcgaat
    36721 acgaccatgg aagcgtgcag ccatcggcgc tcgcgaacac gagtggcgat ccatgccagt
    36781 gccgcggctt caaccgccca cgccatcgtg atacggtacc cggcaaactg tatcgggatc
    36841 gcaagcgtca gaaagcccaa tcccaacccg gcgcaaagca cgccggcgac ccctgacgtc
    36901 cccgccatcg ggcgcagtcg cagaccgagg gccaagtgta gtacgccgag tgcggctgcg
    36961 agaagcccgc gccaggacga gccgtctgct gagagaattt ggtagcttgc cgcgaagtac
    37021 accgtgccgc tgagcgccgc cagcagcatc tccaccttcc caggtccggc gcggcgtgcg
    37081 aacactcgcc acggcagcca ggcaaagtgc aagaaaaacg cgaccgtaag cagttcgaat
    37141 accgcgcccg tttggactgg agactgccct cccccgcgcc acgccgcgta ccacacccag
    37201 aaaccggcga aagagatcag cgagagatac cctcgctctc gccgttccgc gacgacgaac
    37261 cccgccgcgc aaagccccag ggccgcgatc agataggcgg tattcggagt ccagatcgcg
    37321 tacatcgccc cagccgccag aaattgcgcc gccgcgagga cgacgcgtcc gcgggccgtg
    37381 ctgaagagca ggtaatacgc gactgtgtag acggtggcga caaacctctt ctcgttgctg
    37441 agccggtctt ccagccacaa gccgaataga acggctgtcg cgaagaaggc catcgcctcg
    37501 atgccgcgcc actttcggga tttcgagaca gcgaccgctc cggcgcttag aagaagaacg
    37561 taacccagga agaaccacgg gcggtcttca tctgtgctca acagcaatgg cgttgcgtag
    37621 ccgccgatga gaccgagcac gagtaccgcg cgcgactggt agaacagggc aatcgcccca
    37681 gccatcagcg tggtggacgc catcagcagg aacgccagcg gctgcgagat gagcccatag
    37741 aagccgaagg cggcataaaa agagaggtag aggatggcga cgcccgtacc cgacaacccc
    37801 tgcgaataca ccctgtggcc gcggcggaac aaaaggtgtc ccaacccgag ggccagaagt
    37861 ccggcgagga tgcccagaag aattcgcccg cgctctccga tccactggtt gtccaccgcg
    37921 tacttgaaga agaaggccgc tccgagggtg agcgtgacgg ctcccacgcg gttgatcaag
    37981 gttagcccga accgcgtctc cagtccctcc gaggaactcg tcatcgccgg tgcaggcccg
    38041 ggagagcccg gctcagccgc ttcttcaaga acgggttgtt cctccgccgg caccggttcg
    38101 agtgtagggg ccggttcagg cggcacggca cgcgaaatcc ctaacgaggc ctcaacgtca
    38161 tcgaggcggc gctccaccgc atccattcgt cgcagcaggc gcaggatggc ctgggttaac
    38221 gcatccatgc gctcatcggc cggtaaacgc atcgttccac tagtgtatga acagcttacg
    38281 gccgccgcca ctcccaattt cgagttgggc ttgtttacaa gaggaatgag gcgtgcccgg
    38341 aaagaagttg tgccataatt agggcaacgt taccgggagg ttctccctat gaaacagcag
    38401 caagtccttc gagccgctgt cggattggtg ctagcagcgg gtctcgcact cgggcagcgg
    38461 tccggccaga cccaacctcc aagcggcggt gccggcggca acacgggcgg gacgattact
    38521 ccgaccccta ccccaacacc gacgccctcg cccaggccgg attccaggga ccgattccca
    38581 cagccgggtg agcagcaaca gcagcctacg ttccaggaga tgcgccgccc ggtctttctc
    38641 acgggaaaag tgatcctgga agacggcacg cctcctcccg attcggtcac catcgagcga
    38701 atgtgcaacg gtctggcgcg tcccgaggga tacaccgact ccaaaggacg cttcagcatc
    38761 gagcttggcc ggaatacgat gatgatggcc gacgccagcg tctccggtgc caacgaccct
    38821 gcattcagtg gaatgggcgg gttgagcgcc agccgtgggg ggttcggcag cacaagcaac
    38881 cccgccggcg gcctaagcga gcgcgacctg atggggtgtg agctaagggc ggcgctaccc
    38941 ggctatcgct ccgacgctgt catgctcacg ggccgcaagc tgcttgacaa tcccgacgtc
    39001 ggaaccatca tccttcaccg gatgggcaac gtcgagggaa cgacaatcag cgccaccagc
    39061 ctccacgctc ccaaggacgc gcggaaggct tacgacaagg gtcgcgaagc actgcgaaaa
    39121 cagaagtggg ccgacgcgca gaagcagctt gaaaaggccg tccaggtgta tccagagtat
    39181 gccgctgcat ggtacgagtt gggccggtct ctggaaagcc aggagcagtt ggacaaggcg
    39241 cgggaggcgt acgctaagtc attggccgca gacgcgaagt tcgtcaaccc gtacctgcag
    39301 cttgccgggc tcgccgcccg cgacaagaag tggcaggagg tcgccgacac gaccgaccgg
    39361 gtcgtgcggc tcaatcctgt cgactttcct ggcgcctact ttttcagttc catcgcctat
    39421 tacaacctga agaacctcga tgcggctgaa aaaagcgcgc gcgaaggcat caaggcggat
    39481 ccccagcacc ggtttccgaa gatcaatcac atcctcggaa taatcctcgc ccaaaaaggt
    39541 gaattcacgg ccgcggcggg cttcatgaag gactatctca agtttgcgcc caaagccagc
    39601 gacgcggatg tcgtgaagaa tcaactggcg gaactcgaga agttcgccgc cgcccagcag
    39661 cccgcgcagc cgcccgcgcc acaacagtag ccagcagcgg ccactcaccg cgatgtgggc
    39721 tggtcctacg aggcggggtc gccatggatc tccacctcct ccggtctgac cccggcttcc
    39781 gccggttcag tcaaccaccc gcgccagtcc gcccaagccc aaccggcggc ggtgacacat
    39841 cccagaatcc cggcgactac gccgatcgct cgcgggctga agtattgtga cgcgattccg
    39901 gccgctgcca gcgacagcat catcgtcgac caccggagcg actccatcgt ggcgaacaca
    39961 cggccacgaa attggtccgg ggtgtgccgc agcaattgtg agtagttcag caccgacgtg
    40021 accgccatcc ccactcgtga gagcatgacg aacagcaggg ctgccccgta gtgctgcatg
    40081 aggctgaata cgatgtacgt cgacccgtgg ctcaggtagg aaatggtcac ggtgcgcttg
    40141 tagccgccat acgtcgcccg cttcccaacg acgtgtccga tcgcgccgcc gagcaaaagc
    40201 ccgacgccgg cgaatcccca gatggttccg attccttcgg gcccacggcc aaacacctgt
    40261 tcgccgaaaa gggcaaacag tatctgcgcc gcgcctccgc cggctgccca tcccaccgag
    40321 atcatcccga tgccgaagat caggggcacg gagcgcatgt atcgcaaccc gtccgcgtac
    40381 tcgcgccagg ggcgcaccac gtcggcctcc gtcagggcgt accgttgcgc ccggaagtcg
    40441 cgccctccgc cccgcagcat cgaaatggcc agagccgagc acagaaagga gagcgagttg
    40501 atgatgaacg cccactggta accgaatcgc gcggcactga agcccgccag cagcgtgccc
    40561 gccgtcaagg tagcccattg cgtggtctgc gtcagcgaat tggcggcgtg cagttcctcc
    40621 ttgctggcga tcgcaggaag gatggcggtc cggccgctgg tgaagaacgg cgaggcgaac
    40681 atgagcaacg cgctcaacaa gtagagcggc cagctctggt ggctacgaat ggtgaggata
    40741 aagccaagcg ccaccaccgc ccgcacgagg tcgctcgcaa tcatgatccg cttccggtcg
    40801 aaccggtcaa ggaggacgcc ggcgatcggc ccggcgatca ctgccggaat cgcgcgcgat
    40861 agcatcacgc cgctcaccac gaggccggac ccggtggttt ccatgactaa tccgaaaacc
    40921 gcgatgttgt tgaagtaatc gcccgtctca ctgaccacct gcccgagcca cgtatatcga
    40981 taattccggt ttcgccttag tagttcgaga aattccgcca tggcgcgccg tcccgccgct
    41041 tcgggtcacc cacaatctta cagtgggcga accggcattc gttccggttt gcgcaggttg
    41101 agacggcgcc gcacagcgca gcataatgga attgagagca gaaaaaagga acgacgcagc
    41161 caccgatgcc cgcccatccc gcagaacggc aggccggctc gcttattgcc gtaaccgcca
    41221 cttttacggc cgagttggtc cgcgagactc tggaattctg gacagagcaa ctcggcctcg
    41281 acctgtccgt ccggtttgcg ccatacaacc aagtgttcca acaattgctg gacccggcgg
    41341 gattgcccgc ccgcaaccgg gacggcgtga acgtcgtgct tctgcgcttt gaagattggg
    41401 tccggttccg gaacggtttt ccggtgaact ccgaggatct cgagcagaac gtccgcaact
    41461 tggtgttggc gctccgtaca gcggcgacct cggttgcctc cccgtttcta gtctgcattt
    41521 gccccgcctc accggtgttc ctgtccaagc cggaacggcg ggggctgctc gtcaatctcg
    41581 agtcgcttct ggcgtcgtcg gttgcggacc tgggtatggt tcacgtcacg acgcccgcgg
    41641 acctggacgc gttgtacccg gtacgggatt cctacgaccc acatggcgat gagctgggcc
    41701 acatccctta cacccccgtc ttcttcacgg cgctgggtac gttcatcgcc cgcaagatcc
    41761 acgcgatgcg ccgcgctccc tacaaggtga tcgctctcga ctgcgacgat acgctgtgga
    41821 gcggaatctg tggcgaggac gggccggagg gcgtgatcat cgatgccccg caccgcgccc
    41881 ttcaggactt catggttgcc caacgcgacg ccggcatact cctctgcctg tgcagcaaga
    41941 acaacgagga agatgtgatc gagacgtttc gggtccatcc ggagatgccc ctgcgcctcg
    42001 atcagtttgt cgcccgccgc gtcaactggg aggctaaatc ggtcaacctc gccgctcttg
    42061 cagacgagct cgagctcggt ctcgacagct ttatcttcgt ggacgacaac ccgcaggaac
    42121 gagcggaggt gatggccgac cgccccgagg ttcttactct gccgatgcct gctgaggcgg
    42181 acgagatccc gttgtttttg aaacacgtgt ggggattcga ccacgtccgc atcaccgagg
    42241 aggatcgccg cagatcggcg ctctatgagc agcgggccga gcgggcgcgc ctcgaaaaac
    42301 aggcttcaag cttagaagag ttcatctcat cccttcagat cgatatccgg atcggcccga
    42361 tgtcggaagg acaactcccg cgcgtctccc agctcactcg gcgcaccaac cagatgaaca
    42421 tgaccacgat ccggcgttcg gagagcgaaa tccaggcgct gatcgagtcc gggcaagccg
    42481 aatgtctcac tgtcaaagtg agcgaccggt tcggcagtta cggcctgacc ggtgttgtga
    42541 ttttcacgca acggcccgat acactcgggg tggacacctt cctgctgagc tgccgcgtac
    42601 ttgggcgagg cgtcgagcac cgcgtgctgg cccggcttgg agagatcgcc tcggaacggg
    42661 gcctgcgggg tgtggacgtc ccgttcgtgt ccacgcagcg gaaccggccg gcgctgctgt
    42721 tcctggagag cgtcggcgcg cacttcaagg agtccaccga tgggcgcctg ctgttccgct
    42781 ttccggctgg ctacgcgcgc ggagtcgtct acaaacccgc tggaccgccg gcgagatccg
    42841 ccccgcagca cgtggcggag ggacggaaac cggaggcccg cgaacacgtc gactacggcc
    42901 ggatcgcgac tgaactgcgg gagcccgccg cgatcatgaa ccggattcga tccacgaaag
    42961 ccgtgccgga gattgccgcc gtcgataaga cgcagcccac cgatctcgaa gaacggctgg
    43021 tgaggctgtg ggccgaactc ctccggctcc cctcggtggg cattcacgac aactttttcg
    43081 atctaggcgg gcactcgttg ctcgccgtgc agttgctctc gcgcgtgcgc caggaattcc
    43141 aggtggacct gtcgctcgaa gtcgtctaca gcagcgcttt cacggtcgcc gaattggcga
    43201 aagcgatcga actatcgcag atcgagcagg ccggcgccga ctattccgcg attctcgccg
    43261 agatcgaggg actctcggac gatgaggttc gcaatttact cgaacgggaa cagcaccgtg
    43321 cgccgggccg gaaggacgaa gctcgctgag cgatgaggat actgctcgcg gccaacgcct
    43381 cttatgtgcc gccgagaggt ggcgccacac gcagcaacct agtctggctc gaacatctcg
    43441 ccgccgccgg ccattcctgc cgcgtggtcg cctcggaact cgcgcacgac cgcgtcggca
    43501 agctggagca gatccgcaac gaggaaatgc gtgtcgttgt ttcccagctt gacgcggccg
    43561 acggcgttga ggtggtccgg catggctccg tcttggtgtt ttcatccgcc gatccggccc
    43621 gcagaggccg tctgttgcgc gagcagatcc ggggcttcca gccggactgg gtgttggtgt
    43681 catccgagga cctcggccag gtgcttttgc gcgaggcggt gcacgaagct ccgggccggg
    43741 tggtctacct ggcgcacacc ccgcagttgt ttccatttgg accggcgagc tggaatcccg
    43801 atccaaacgg cgcggaacta gtgcgcctct gcgccggcgt ggtcgcaatc gggagtcaca
    43861 cagcggagta catccggcag cactccggat gtgaggtgat ggtcatccat cccccgatct
    43921 atcccgccgg accgctcgag aagttcggca acttcgacga aggactgatc acccttatca
    43981 atccgtgcgc cgtcaaaggc atcaccattt ttctctcgct cgcccggcaa tgtccggagt
    44041 actggttcgg cgcgctaccc ggctggggga cgacggccac cgaccgcgat gcgctcgaca
    44101 gcctgccgaa cgtcacagtg ctggcgaatg tgaaacagat cgggcaggtg ttgcggcgga
    44161 cacgcgttct gctgatgccg tcgttgtggt acgagggttt cgggttgagc gtaatggaag
    44221 cgatgctgcg cggaattccc gtgcttgcca gcgattcggg cggtctcgtg gaagcgaaga
    44281 tgggcacgcg gtttgccctt cccgtgcgcc ccatcgcacg ctacgaggcc gttttcgacg
    44341 agcatggtct gccgatggcc gtcgtcccgg aacaggacgt ccagccttgg gtgggagctc
    44401 tccggggact cctctccgac cgtatcctgt acgaagacga atcggaaacg tcccgcgaac
    44461 gcgcgtgcca gtttgtcgac agcatccggc cagaacaaat ggaagagtat ctccggagcc
    44521 tgactcacaa accggcggag cggctacgtt ccgcgatggt tacttcagga ccgcacgacg
    44581 ctctgcgaca cctctctccc gagaagcggg cgctgctgtt gcagcggctg aggcacaagg
    44641 cgtccgccgt ccgaaacggc gtgcactgaa gtcgcggcca tgcgcatcct gctcgcccac
    44701 aactcgctgt actatccctc tcacggcggc ggtgacatct cgaaccggct gctgatggaa
    44761 gccctcgcaa cgcgaggtca tgcgtgccgc gtggtggccc gcctctccac ttttagcgaa
    44821 gacgagcacc gtcgcttcgt ccgggacttg gcggcgagac aggttgaact gaaatcctcc
    44881 gctgcgggcg cggctgtgtt cgaccaccgc ggcgtcgagg tgcacgtcct taccgaaaat
    44941 ccgaacttgc gcggctactt cgccggccag gttagggagt tctccccgtc ggtgattctg
    45001 gcctcgaccg acgacccggc tcaactgctt cttgaagcat cactgctcgc cgacggcccc
    45061 cgcgttgtgt accttgtccg cgcaaccctt gccttgcctt tcggccccga cgccgcattt
    45121 ccgagcgaac tcaaggccca gatgctgcgc aacgtcgacg gggtcgtcgg tgtgagccgc
    45181 tacgtggccg gatacgtgcg ccgctggagt ggtatcgaag caatccatgt acccatttcg
    45241 ctgatggaac ccgggccgtt tccggatctt gggcggttcg acaacgagtt cgtcacgctc
    45301 gtgaatccct gtgcagtgaa aggcatctcg atcttcctgg cactcgccga acgaatgccc
    45361 ggcgtgcagt tcgccgccgt gcccacctgg gggaccaaca cgcaggatcg agccgcgctt
    45421 caggcgtacg ccaatattgc cgtgctcgat ccggtcgatg acatcgacga gatcctgaaa
    45481 cgaacgcggg tgctcctcgt tccgtctctc tgggcggaag cccgttcgcg catcatcgtc
    45541 gaggccatgc tgcgcggcgt tccggtgatc gcgagcgacg tcggcggcat cccggaagcg
    45601 aagatgggcg tcgattatct gctgcccgtg cggccgatcg aacggtatca gccgaaagtg
    45661 gacgaacaga tggtgcccgt tgccgaggtt ccaaggcaag atatcgggcc atgggaggag
    45721 gtccttcgcc gtttggtcac cgaccggcgc cactacgagc agctttcccg cgcgtcgcat
    45781 gaagcggcgg tcgcgtatgt cgagaacctg agcgtggcgc cgctcgaaag ctacctggaa
    45841 gagaccgtcc gggcgcctgc gcgtccgcgc ctctttgcgc ccgtggagac agccgcgccc
    45901 tcacccgtcg acaagctgtc gcccgaaaaa cggcggcttc tcgcccttcg tttgcagcga
    45961 accgtctcgg ctgcgacgcc atccgactcc tggttcccga cggcggtctg gagggagcga
    46021 aagcgcctgc gcctgttctg cttcccgtcc gccggaggag gaacgtcgtc attccgaggg
    46081 tggagcgaga gcttgccccc cgaggttctg atctgcccgg tccgtctacc tggccgggag
    46141 agccgtctct ccgagcctcc attccggagc atggagactc tgattgccga actagcaaag
    46201 gcgatcgaac cctatctgtc acaaccgtac gcgttcttcg ggcacagcat gggcgcggcg
    46261 gttgcatttg aactggtccg gcgtttacgc cggcaaggcc tgcctccgcc ggccggcctg
    46321 ttcgtttctg gcgctcgcgc gcctcagttc cggttgggcc atgtgccggc cccggaaccg
    46381 tcagacgagg agttcttaga cgagttgcgg cggctcgagg gtgtccccgc ggcgattctt
    46441 gaaaaccggg agcttcttca ggtgatactg ccggcactgc gcgcggacac ggaactctac
    46501 cggaactacg tttacgttga ggaaccgccg cttgcttgcc cgattcgggc ctatgggggc
    46561 gtcggcgatc cgaacgtgaa accggaacac cttggggcgt ggggccgcca aacgacagtc
    46621 ggattttcgc ttcggacctt cgatggcggc cacttcttcg tggacagcgc gcgtgacgag
    46681 ttccttcgtg cgcttgccgg cgacctgggt gaactggtca gctctgcgtc atgatctctc
    46741 ggacgaactg ggcggtctgg ccgatctgat cgttgaaacg ctggtagatg ccgatgatga
    46801 ccgggtccgt cggcttgata ccgttcaagg ccacggccat ccgctcgcgg atctgcttcg
    46861 gcgagtccac tgtccggccc gctgccagca ctttgtacgt aaggcatggc ttcggagtct
    46921 tgcggatcgt ctccatcatc ttcggaggat ccgaagctag gtagatctct cccagtggga
    46981 tctctcccag gatcttttcg aactccgcgc ggggccggat catgtagtag agcgcggtca
    47041 tatagtagtc gacgtcccag cccttctcct ccgcgtactc gactgccttc gggttgtggc
    47101 aggagaggcc gaccatggcc cccgcatcgc ggatgtgctt caggtagtcc cgagacttct
    47161 cgaactgccc cgagtgccaa agccggttgg tcgagccgcc gtgctgggca atcccgagtg
    47221 gctgtagctt cattgcccgc ttcaccatct ccggctgctc gttgaagttc ggccgcgaca
    47281 gaatcagcaa ctggagcttc ccgcccttct ccttgtactt cagccagtcg atttccagtc
    47341 gctcgctcca gctcgcctgc caggtgttga cgcctgcccg ctccaactgt tcgagaaagg
    47401 acagcacgcg cggagttgtg tgatactcgc ccatcgtcgt cgagaacaca tagttgaagt
    47461 gcgagtaacc gtagattgtg ttcgctccgg cgatcagccg cgtgagcttg tgcttaccaa
    47521 acgggacggt cggaagcatt gggccggcct gtgcttcggg cacgcggtcc gccggttcgg
    47581 cctgcgcgac tccctggttg gccgcggcga gccatgctgc gaccgttccg gctccccgaa
    47641 aaagatcgcg gcgttccaac acagaatcgt ccaacggatc gatttcaatc atgggtccct
    47701 ctccagaatc agtttcgcta attatgtcac agccgtgcgc gcaggtaggg cggaccccgg
    47761 cggcaagttg tccgccgtcg cagtcagaaa cgacctccga gctgggagcg gctgcggcca
    47821 ttgtccgcgc gatggctgct taaaccgcgc tcgacatgct gcgccgaacg gtgtagcggg
    47881 gtcccgagaa accgggcagc acaacctgag catggggcgt tggcacgatc gttggcgacg
    47941 gagtccggag gacaggtgag tttggcaatt tcgtagcctg tcacctaatc ttggtgggtg
    48001 gcgctgccgg ggcttcaaac gtgcacctac ggctggcttc gctcggctaa cattccctcg
    48061 atttcgcgac ggacaccgtt catccaccga ataacaggca gattgccctg ggattcgcgg
    48121 ccgatctcca cgtcctgaaa cgagaaactg agggtattga accgttggga gtattccaac
    48181 ctgtagatag tggggtcaag cattatccca ccttcgaacg ctgagttctc gagggaagtc
    48241 gtcgattcgg caaatgaacg ccagaaaccc tcgacccatc gctccgccga agcctgggaa
    48301 accttcagga tggagtgtct tacctccatt tgcttcgcca ccgccgtggg gtctgggtcc
    48361 cgctggccag cgaaggcact catcaagact tcatgcgcgg gtgcccttgc cttggagtac
    48421 cgtattaagg gctcagtgtc tcgcccgcaa cggatggtaa tttgcagctc cgggtgaaaa
    48481 ctgggcaaga atcggagcac tagtgagaat tgcgtcgttc tccaatcttc cttggcactc
    48541 gcgaaagcct tgtcccagac ccgttcgtac gcctcttcag tagggacagg cactcgtttt
    48601 cctcttgggt acgccccctc aagggatctc gccttctgcg gcgtggatgc gccgaaaagt
    48661 actgggacga gggtgcaggc cacagcaacg aaggaccact tggattgacg cggcacgcgc
    48721 gaccggtgca ccacctgttg gcatgtgcgt gtccttagac tctcaggctc ccgttcggtc
    48781 ataacctccg aggaatgcaa gccacggtgt tgtctgacag ttttagcagc agcaacaggg
    48841 cttggggcgg acggtgtcca tgcccgaatg gtgcggatgt gtatcagctc gctcattgag
    48901 ctctcttata taagacacag acacaaatcg agcatgcctt agacggaccg tacataactt
    48961 tctcctggaa gccgccacgc tccggcggcc ccaaaaaaca ggagcataat gtctcaaact
    49021 ctgaataatg tcaaggttca atttttcctt aatgcggccg acttacagaa aacaaaggaa
    49081 atttagttgc cgactcactg ccgtcgttga gcgggcagtc aatgaaacca cgtctgcacc
    49141 tgggggtttc gcggactcta gacttctgta tcgcgcactc cgtcacagca gcgcgcttga
    49201 ctgtgctcat ggaagcggta tatacgtagc ccgaggtccg aaaacagcga tgtccttatc
    49261 tctccgaaac gcttcatgtg tgtctgccct gctcgccctc gccacggtgc tggctgccgg
    49321 cgccaatcaa cccgaaaagg tcgtgcccgc gatcaacgac gtggagatct ggcagcaggt
    49381 cggccagcag ccctatgagt tcacctgggt gcagcgcgaa gagcatcccc agacgctggt
    49441 cgacttcgaa gacctgaaag actggcggct cgagatgttc gatggcggct ccggcgaatt
    49501 ccgccgtagc cgtgagcagc agttgtgggg acagtacgtc ggcaagttcc tttactccgg
    49561 cagccgtccg gggagccgca tcgtggcgcg cccgccgaag ccgatcccga tcccgggcaa
    49621 gttcgattcc atcgacatct ggggttacgg acatcgctgg ggacgccgcg tcgagcaagt
    49681 cacaccgcgg gtcgacatct ccgtatggct ggtggacgcg cgtggcaaag agttcaagtt
    49741 cttcatgaac gacgtgaagt ggcgccagtg gtggctgctg caccggcgga ttcccaagga
    49801 agtgcaagcg caaatgttga tgcccgccag cttcgccgga atcgaagtcg ccatgggcga
    49861 gacccgtgag ccgcgttatt tctattgcga ttcactcgca ttctacgtcg aggatctgaa
    49921 gccccttgcg ctgaagccgc agccgcggcg caatttgaaa ccgtttcgag ggcagatcgc
    49981 cggactgaac accggtccgg acaagcttcc gttccccacc cgcgaggaga cgatccttcc
    50041 ctcgaacttc gagaaggact tccaggtttc cgtgcgccag accgggcctg ccggattcga
    50101 gcttcaatac aagggacgcg acgcgacgac cacgtataac tacaggccgc gctcgggaag
    50161 tcttagcgaa ctgagcgccg ccatcgacgg tggcaagccg tttcagcctc tacacggcgg
    50221 tggcgtccga ttcaccgaca cgcccaagga tcgagtctcc gaaggcgagc ttctctcggc
    50281 ggagctcgcc ggtgacgtga tcaaggccaa gttccgccac ggctcgcgga tcgtggacta
    50341 cgaattgcgc ctgtggcaga agtcgatcgt gctggacgtg tggtgcgacg gcggcgaggc
    50401 cgtcgaactg agtttcgggc gcatcgccgg tgtcacgaat ccgaagatcg tcccggtgcc
    50461 gtacatcagc taccgttaca cgaacccgcg tgtgttgatg tcgggaagtg agacaaagcc
    50521 gctgttcacg tcagtctggt tcgactggta ccgcagcaac gcctccgagc cgtactgctc
    50581 gcccaagccg ctgcttaccg ccgatggcgg cgaggccaac ggcggtatgc gctacatccc
    50641 gaagacggac ggtcttcgca ataacctcta cgaacgggtc ttcatcacct cgtcgcctgt
    50701 ttacgaggaa acatttccga ccatcgccaa ccctccgtcg ctggcgcagg agaaaggaaa
    50761 gcaggtcgtg tggacggtca ccgcgccgcc cacattccag tcggaccaca agcgcagccg
    50821 cgagattcgc tcctacggca tggacaagat catgcagcac agccacgagg tgacctggcg
    50881 cgacgacgga gacagcttca ccatgcggct gaaagcggcc ccgcaaaagg gcggagacgc
    50941 gatgctgcgc tggtacctga aggcgcagaa cgacatgggc tggacccagg gcgtctacac
    51001 caactacacc gatttcgcgc cggtgaacac gaactggagt ccggaccacg tgcagcgtct
    51061 gcccgacggc gagtggcgcc gggcatggcc gcgcaactac gcgctgaagc cctccaaggc
    51121 cgtcgagttc gacgagtact acgcgcagcg gatcaagcgg aaattcaacc cgatgatgtc
    51181 gtacaccgac gtccacaccg cggtaccgcc atgggagtac tgtgatttcg acgcccgcgt
    51241 ccccggagcg ggaacgttcg cagcgacatt ctacgcttac ggccagttgt tgctgaacga
    51301 tcagaaggag tacggcgtct gccagaccga ggccaccttc cagtggcttt atgcgggcct
    51361 cgactcaggc agctacggct gggtgtattc ggacatcaac attcttggcc atccgttgga
    51421 tgtggcgttc cgcctgcaca agattcatcc cctacattgc gattacggca taggagcgcc
    51481 cgaccgccat ctcacggaac tggacccggg ctggaggcag tcgccgaagc ggcggcagta
    51541 tatcgatctg tttctcgcct cgacgatcgc ctacggcaac atgggctggc tggtgaagga
    51601 cttcttcgac gagaggcctt tcggtatcga ggtgatggcg cggtcgtatt acatgatgca
    51661 gcagttgcag cagcagtacg cgtatgttcc ggtcgcgaag atcgaatacg cggacggcgc
    51721 cgggcggttc gtcacgccaa gccaggccca agcgggcggc gcgctgaagg attcgaaggt
    51781 ccacgtcgtg tacgaaaacg gcacggaagt gtacgtcaac ggctcggcgt cgtcgccctg
    51841 gagcgtcagg gacagcaacg gcaagagtgt cgaactgccg ccttacggct ggctggtctt
    51901 caatgccaag tcgaagttct atgagatgtc ggcgaacgcc ggcggcaggc gtatcgacta
    51961 cgtcactgcg ccggagtacg agtacctcga cggccgcggg cagtggagcc agcagggtaa
    52021 tctcggttcg gccggcgcgg tcgcgctgcg ccagaaggcc gaaggagtgc tggagttaat
    52081 cgacatttac ggaaacggcc ggatcggatt ccgcgcggcg ttcgacggcg tcctgatggc
    52141 gtacgatccg gacggtgcac ctctcggcaa ggtcgagacc tcgccgcgcg agggatggtg
    52201 tgagttcacg ccgctggccg gggcgcgctc ctacgtcttt gcctcggctc agtagcctcc
    52261 ggctactcgc ggccaggccg gaaatagtaa ctgtacacgg cgcctagaac gatcagcagg
    52321 gcgccccacc acaactcggt gtgaaggttc gccatcacga cttcgcgctc gggcgggctg
    52381 gtgagtccgt agaggccgaa gccgagaatg atgacgccat aaatcagcag cagaacgccg
    52441 atg
//
LOCUS       DATIFQ010000146        19941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2348030, whole genome shotgun sequence.
ACCESSION   DATIFQ010000146 DATIFQ010000000
VERSION     DATIFQ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19941
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348030"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VLE22_06930"
     CDS             complement(<1..961)
                     /locus_tag="VLE22_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14176.1"
                     /translation="MWRNLCFALRVLKNSPGFTAVAVMTLGLGIAANTTVFGWVDTIL
                     LQPIPGVRNAHELAALEGVAPDGGRLGQFPHPDFRDFQRQMTQSSGVVAAHVAFFTIG
                     PPDHPQRVLGQVVSANFFSVLGVEPFLGRILTPEEDRDIPGAFPNAVISHRLWRTYFR
                     QDTGVVGRAVRINGHPYTVVGVAQPDFAGTIGGGALDVWVPLSMIIQTGTLNTWAASD
                     RNARFLEVIVRVKPGVTIEQARDEARAVAVRIAAAHPDTHKGVGASLVPIWRASYGLQ
                     SNLRDPLRILMAVCILVLLIACANVANLLMARSVSRQREFGIRI"
     gene            1312..2403
                     /locus_tag="VLE22_06935"
     CDS             1312..2403
                     /locus_tag="VLE22_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HSB14177.1"
                     /translation="MECPAELRPSCLITFLWRGDESTQNVLIRSEAMPGAPAEHLFDR
                     IPGTGIWYRTYLFRDDARFMYMLSVNDPLTPWDVQGPERRKRYAGVRTDPLNRYVTND
                     PRSASYVSLPRAPSDRWIEDRAGVAHGELQTHDLPSVALSGKRWVDVYTTPGFQRGAG
                     VTPVLILFDGEEAKALSKTPIILDNLFAERRIPQMIAVFLAQPYERRETDLACSEATN
                     VFLTKELLPWLRGEYRIRTAADRTVVAGASLGGLAAAYAALRHPEKIGRVLSQSGSFW
                     WGRTDAEPEWLTSEFKSKPRVNARFYLDVGLMETEGNAISQLETNRRLVAVLRSKGYK
                     VVYHEFNGTHSYPCWRAGFADALTALLSD"
     gene            complement(2433..3587)
                     /locus_tag="VLE22_06940"
     CDS             complement(2433..3587)
                     /locus_tag="VLE22_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HSB14178.1"
                     /translation="MTRLLSTLIVGGALLPAPFIATCAGGSTDPSSVLIVINDNTPPE
                     AGTEGVNAGQYVAARYAAARGIPAGNIAHIRTTMACCDSDPRAWDSWNITWQEFVNEV
                     REPLKAFLKASGLARKIHYIVPVYGVPSHIERHPYGATGLSVDAFLTLMSSRYADVIP
                     MDNPAYSPDPQVMPRFPRNAYGGPYYIVSRLDGPSAEIAAGLVDKALAAESGISRRSG
                     VGYFDWRHLSPSSGGYYVADQTMIGGYELCVSAGLDCILNDQTATGGMIASAPGALWA
                     WGWYSFSTNDVYSFAPGAVGAQLTSYTAESIRYQKPGAWVPLWLERGITATWGATGEP
                     YVNGYTVGDALLNRLWNGYTFGEAAYLATPKLNWKMVFVGDPLYSPRFID"
     gene            3709..4986
                     /locus_tag="VLE22_06945"
     CDS             3709..4986
                     /locus_tag="VLE22_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14179.1"
                     /translation="MTENACWDRGKDGWKINMGETDSAINTPVVRVGIAGAGMAGSFH
                     AECLRRVHGVRVKLAGVTSLRPESRREFGMSRGIPVFDSLEEMIEHVDVVDLCSPPYA
                     HKDGILSAARAGKHIICEKPLTGFFGPPADDGFRGNAFPKEKMLDEVCSSLREIASAV
                     SEGGKCFGYAENFVYAPAVQKERQIVEKSGAQILRMLGEESHKGSASPVYGIWRYAGG
                     GSLIGKGCHPLTALLYLKRSEGLARDGSPIRPVTVSARTHELTRLPGYRDRKFLRTDY
                     LDIEDCGWMHVVFSDGTIGDVVTGELTLGGIYDYVEVFANNHRARCRLSPTNLMDVYN
                     PSGREFDGIYLMEKISTQEGWSNAAPDENWTMGYQPEIQDFIECAATGREPQSGLALA
                     LDTTLTIYAAYVSAERRGAEVEVPLLAEETARA"
     gene            complement(5041..6234)
                     /locus_tag="VLE22_06950"
     CDS             complement(5041..6234)
                     /locus_tag="VLE22_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HSB14180.1"
                     /translation="MPNPFHEAHFLSVFQSNFRPFNGAVASELTRLPIASPASGFTYV
                     FDSATGVFTRSAQSFGPILTERAETIGRGKFFLGFAYQRFSFDSIDGIPLDSMPTVFT
                     HEQETGAEYEKDFVTARNSVDMTLNQFTIFGTVGLTNRIDVSVAIPILDLKMKVASDA
                     QIHRIAPPNPTFGQAHYFDSQNRDGSIAKTFTSSGSATGIGDVIIRLKGTVKRWESSS
                     FAVAADLYVPSGDERNFLGSGAFGLRPFIAYSARVGRVAPHFTVGYQVNQSSLLAGDI
                     SANRKGHLANQFTYTAGADVGVARPLTLAFDLLGQRVFKAARALPTTFVSPAPELSNP
                     GAGQVTYANTRFATESFNITSAAAGAKLNIAGSLLLTGNLLFRLDDGGLKDKVVPLIG
                     LSYTF"
     gene            complement(6475..11601)
                     /locus_tag="VLE22_06955"
     CDS             complement(6475..11601)
                     /locus_tag="VLE22_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSB14181.1"
                     /translation="MAEASIKHIGRYEIQAELGRGGYGRVYRAYDPTVGRLVAIKVLT
                     GEGTDLLKRFRNEASAAGSLNHRNIVTIYDFGEHDGMPYIAMEFLAGDDLSQIIANRT
                     PLTMLQKVNIMMQVAEGLYSAHRNGVVHRDVKPANIRLLPDGTVKILDFGIARLTRDS
                     TSPRLTRQGDVIGTLLYMSPEQIMSADVDALSDIFAYGVTYYELLTGKHPFHAADAGA
                     VLYKITSVDPEPIRNHTPECPEALEQVVRRALHRERELRYQSLRELQIDTEPILLELR
                     QKRASVLLDDARNCFAEDDLERAEAHVMEALALDPSNTAGRKFRNEVQQRLQSRRLQS
                     RIDAILAEGDHALKQRRFAEAAERFQSALQVYPENEKLKGRLQQARELLERSRAAAQL
                     LAEARKHFFQKGYSAALRIASEAAQIDPECTDVARFVQSVQTAAEREERRLRFQQVLG
                     AAQELLDQNLFDQAAAALAELDSEQSDSAEAKALIESLNTRRAEFERAEREQAERIRT
                     ELAAATEELRNSAFLQAFERLSTLRREVPENEDIGRLLALAEKELSAQKRAAEIEVLV
                     REARSFVESDQYGRALGVIEDALRSFAGDARLLQLLEEVKAGQERIASIRSALAEAKT
                     RLEAGRPEEAVQVLEPVSAKFTGEAQLEQVLRQAREALQKRQKAEAVERAEREVHALV
                     EAEKFDKALDVIEAARRQHGGDPGLAELTERIGRAREERHRAVAVQTAAANAEALIQA
                     GKPEEAIRVLEPASAEFTGEAQLEQVLRRAREALQKKQKAEAVERAEREVHALVEAEK
                     FDKALDVIEAVRRQHGGDPALARLTERIESVRERRRRAVAVQTAAANAEALIQAGKPE
                     EAIRVLEPASAEFAGEAQLEQALRQAREALQKKQKAEAVERAEREVQSLVEAGEFERA
                     VAVIAAVRREHGDDPALAPLTERIAKGREERRRVAAVQTAVANGEALVQAGKPEEAIR
                     VLEVASVEFTGEAQLEQVLRQARQALKQKQKAEAIEHAVREVQALVEAGEFDKARKSL
                     KAALKKHPQEARLLELRNQIQTARAGRELDEARPPQEVPQQLEPKLAERSPLITPRRA
                     IAVAAVLVAGVGAVLVIRQINAPPGGTIPVEVRTSPAGAVVRYKDGSCTPPDCRLQLA
                     PGDYAFEVQLPGYRTETRKTTVVKNSAPVSLEIALSALPSTVQLTTTFADGSVTLDGR
                     TAGRLRDGQFIADNLGPGRHELEAKGGDGDVKLTFETAEAKRPLLSSPPSTRDADAVV
                     VTAFGNEVAVNCARCNGEAVLDGTLVGRFVGGQLTLKGVSAGTHELQVGGGAAPRKLV
                     FAVGTQPSLVTYLTSARDRGILLVQTGLDDAVVYIGGKQWPRRTERGELSIPLDAKEY
                     EVRVEKQGYRVVPPVVRAAVRKGEQVRAPFRLEPLEAVLTITGQVPGATVAVDRKLAG
                     AVGGNGVFSVQVQPGERRIELSKDGYQPAAISRSFSPGERLQLGTDQLRLQPIPRPAP
                     EPAPVKAVSPPPPPQPSPAELEAQQWQHVRDSRDISDLAEFLTRYPGGRFAREAEARI
                     EELEWSATNRNNLASLEAFLAKHPRGTFVGEARKQIAQLQQEQQQRSEGAAVRATVSR
                     YEEAYRRRDAAGVRALYVNMPNNQFDQIRKSFSVFQVISFQLKPEEPQVAGETATVRC
                     VRMLTVKDNRGAEQTANDVALFRLKKEGNEWRIVSLSPLN"
     gene            11872..12630
                     /locus_tag="VLE22_06960"
     CDS             11872..12630
                     /locus_tag="VLE22_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HSB14182.1"
                     /translation="MISACGLSDTGCVRTDNQDRLLVDDELGFYAIADGMGGHSHGEV
                     AAELAISTMKYYLESSHDRADISWPFGFDFDLSVDSNRLITAIRLANHQVWKRAEEKP
                     EYAGMGTTVAALLIHDNQAAVANVGDSRVYLFRNGDLKQITTDDTWLNAILQRGPNYA
                     QLRSHPLRNMLTQAAGSQSDVSVHNAALEVQAGDRLLLSSDGLHGVVEDEEIRGILAT
                     GGSIEELAARLLGAARTQGAPDNVSCILLSFGDR"
     gene            12636..13628
                     /locus_tag="VLE22_06965"
     CDS             12636..13628
                     /locus_tag="VLE22_06965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSB14183.1"
                     /translation="MGELFGRYEIIEEIGRGAMGTVHRAHDTVLDRAVAIKRMRSGPR
                     VDPELTKRFYREAQVGARLQHSSIVTVYDLGEHEGTSFIVMELLDGIDWRKVIIERRA
                     MPAAKRIELMAQVCDGLAHAHRHTIVHRDLKPSNLFIHQDHQAKILDFGLARMPTSSL
                     TLTGRVLGTPNYMAPEQILGQRVDSRSDLFSAAIVFFEFLTGIHPFRGPIVPRQIVDG
                     EPERLVNINPLLPDSLGLIFERALAKSPDARYQDAAELASDLRAAVREVPEEPGAQVP
                     LSPESSSLSPESQTQVSAPDAPLYAADADTETIVYLAPETRPVPPGPNEGSSHK"
     gene            complement(13634..14614)
                     /locus_tag="VLE22_06970"
     CDS             complement(13634..14614)
                     /locus_tag="VLE22_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSB14184.1"
                     /translation="MLGSGFPYLVVPDAGGEREFPLMSGYMWRIGRNDACDIALASKR
                     VSRDHAIIQRDDSGDYYLIDMGSQNGSFVNDRRVSTPALLRDGDRVSIGGFVFTFHWP
                     GGGSDPASSVMAETAATEACFQRQFLTVLVVDIRDFTRLTQRTEQSVLSEAIGTWFRH
                     ASEIMKRHGTWALKYIGDAIMSTWLHSEPGAASRDLAQILQALVEFAEATSALEADFQ
                     LPAPIRIGAGINTGYASVGNTGTRTFTDYTALGDAVNAAFRIESATKDIGADVVIGAS
                     TFEALHFSPVREHHFQPHDARLKGYDAPVRVWAASLEGIKAFLDESPKPS"
     gene            complement(14819..15652)
                     /locus_tag="VLE22_06975"
     CDS             complement(14819..15652)
                     /locus_tag="VLE22_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB14185.1"
                     /translation="MGGNGGLRLKFWGVRGSTPTPQIDNLSYGGNTACIEIRSPDDQV
                     VILDAGSGLRKLGQTLLQESGNSRLSVHFLLTHFHWDHIQGIPFFAPLYRPETDATFY
                     TGQALGSIEEKLQGQMSRPYFPIAFEVLAARRQFAELDDKPFKLADLVIHPFPLNHPQ
                     GAVGYRIEAGEKVIVHASDLEHGHPQLDNVLRQYAEGADVLVYDAQFTPEEYESHKGW
                     GHSTWLEATRVAHEARVKTLILFHHDPWHDDQTVFEIVQQARLFFDNTLGATEGWHVC
                     L"
     gene            complement(15819..17348)
                     /locus_tag="VLE22_06980"
     CDS             complement(15819..17348)
                     /locus_tag="VLE22_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSB14186.1"
                     /translation="MIGQTIKHYKIIEKLGDGRVGPIYKAQDLNLDRIVTLKFLQREV
                     ARDETAKARFLLEAKAASALDHPNIGTFYSMEQTDDGQLFTSFAHYEGVLLSEKIKQG
                     PLPLEQAVDIISQIGRGLAQVHLKGIIHRNIKPGNVLLTREGSVKILDFGLAKLQAAP
                     SALTQAGVVLGTPAYMAPEQLKGGRIDQRADVWSLGILMYLMITGRLPFEADNFVSLT
                     FAIMEDDPPPMSSLRPEATPELERIAQKALAKQPDDRYAEVNDLLADLAAATSRKNAI
                     PVAVRGVDTATMAPAFEAPSQPVLGMAYVLFLDIVGYSKLLMDTQARAVEDLSSVVLS
                     TQPYLRASRSDQIVLIPTGDGMALVFFDSPMSPVECAVEIARALKNHKFIRLRMGAHA
                     GPVYRTVDINKARNVAGGGINLAQRVMDCADADHILVSKAIAEILSELSEWRQNLHEL
                     GIQQVKHGVTVEIYNLYSPDFGNSQHPQRCVPVAPPPVAGVDKKKRSIFQRLIGGSST
                     I"
     gene            17633..19363
                     /locus_tag="VLE22_06985"
     CDS             17633..19363
                     /locus_tag="VLE22_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSB14187.1"
                     /translation="MSFIVSDPVAWLSSIRLHGVLEEIGLSIIIASAVGYVAHRLKQP
                     IILAYLLAGCLLGEKLGVGLLKNPESIELISEIGLVLLLFVIGLELDLTKIMVSGRQL
                     LVAGLGQFVVCVALGLGFFGLWGLSPSQDWLAVLYLSLLASLSSTAIVVKLLYDKFEL
                     DTLPGRLTLGILVFQDVWAILILAFQPNFQDPRIAIIGSTVLKGVVLFVVGFFVSKYL
                     LRFVFESVAKSPEMVVTLSIGWCAAVAGVAQALGLSKEMGGLVAGFAISNFPYGIHVS
                     AKVLPLRDFFLTLFFISLGIKIPTLEPRLVPALGGLVLFVVLSRFLSVYPLLSVAGSG
                     RRTCFITSLNLSQVSEFSLVIAALGVNYKHINDDVMTLVLYALAITSVVSSYTIKYSQ
                     PLFVLYDRALNRIGWRQKGTEAPEVAAAGHGQHSIVLLGFHRAARAFVESLSRAGSPL
                     LKELLVVDFNPEVLSEMRARNVAAIFGDIGSPDTLLHVHLDQARVILSTIPDMLLKGT
                     SNLQLARVCRSLSPNARIVSVADSGAQIQDLIDAGADDVILPYDLHGDRLAEMVQTLG
                     SEQKFDHARN"
     gene            19626..>19941
                     /locus_tag="VLE22_06990"
     CDS             19626..>19941
                     /locus_tag="VLE22_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSB14188.1"
                     /translation="MGSRRDFLKASVGAIAGAGAFWSSLRVAKGQFPPPPPTSPPLEP
                     FVDPLPLPPVLRPVRPGFWLGRWWGLARALGPHYELPMLEVSQKLHRDLPPTRVWGYG
                     GMY"
BASE COUNT         3766 a         6100 c         5984 g         4091 t
ORIGIN      
        1 cgatgcgaat gccgaattcc ctttgccggc ttaccgaccg tgccatcagc aggtttgcca
       61 cgttcgcgca cgcaatcagc aacacaagga tgcacaccgc catcagaatg cgaagtggat
      121 cgcggaggtt ggactggagc ccgtacgacg cccgccagat tggaacgagc gaagcgccga
      181 cgcccttgtg agtgtccgga tgcgctgctg caatcctgac cgccactgcc cgcgcttcat
      241 cccgagcctg ctcgatcgtc actccgggct tcacgcgcac gatcacttcg agaaacctcg
      301 cgttcctgtc ggaagcggcc cacgtattca gcgtccccgt ctggatgatc atgctcagcg
      361 ggacccagac gtccaaagcc ccgcccccga tggttccggc gaaatccggc tgggcgacgc
      421 cgaccaccgt atacgggtgc ccgttgatgc gcaccgcccg cccgactacg ccggtgtcct
      481 gacgaaaata cgtccgccac aagcggtggc tgatcacggc attcggaaag gcgcccggta
      541 tgtcgcgatc ctcctccggc gtgaggattc gaccgaggaa cggctccacg cccagcacgg
      601 agaagaagtt ggccgacacg acctggccca gtacgcgctg ggggtgatcc ggcggaccga
      661 tcgtaaagaa tgcgacgtgg gccgccacca cgcccgacga ctgcgtcatc tgccgctgga
      721 agtcgcgaaa atcagggtgc ggaaactggc cgagccggcc tccgtccggc gctacccctt
      781 ccagcgccgc cagttcgtgt gcatttctta cgccgggaat cggctgcagc aggatcgtgt
      841 ccacccagcc aaagaccgtt gtattcgccg cgatacccag tccgagggtc atcacggcaa
      901 ccgcagtgaa tcccgggctg ttcttcaaca cgcggagagc aaagcacaga ttccgccaca
      961 cgtttgacat tttgccacca tgaggcctgc aattctgata ccagccggaa gacgctcatt
     1021 tcattcgttt agggccgcga gtcggtgtcc gattccggga ccgcgattca cgcaaccgga
     1081 cggtcctctg ccgcccgccg ccggacgaga gggaaacctc gggagactgc atggtgtcca
     1141 atcgaagtgc gacctgctct tctttctctg ttactgacaa tggcagcgtg ggccgacggg
     1201 gttccgccaa gtcccaggat cgaattactc aaggaagcac ttcgggcgag gcaggccaac
     1261 gcagtcgagg ccttctggga gaacgtcgcg acactcggcg gcacgccttt gatggagtgt
     1321 ccggcagaac tcaggccatc ctgcttgata acgtttctgt ggcgtgggga cgaatcgacc
     1381 caaaatgtac tgatccggtc cgaggctatg cccggcgcgc cggccgaaca cctcttcgat
     1441 cgcatcccgg gcaccggcat ctggtatcgc acctacttgt ttcgcgatga cgcacggttc
     1501 atgtacatgc tctcggtcaa cgacccgcta acgccgtggg acgtccaagg accagaacgg
     1561 agaaagcggt acgccggggt ccgtacagat ccgctgaatc gttatgtgac aaatgacccg
     1621 cgcagcgcat cctacgtttc cctccctcga gcaccctcgg accggtggat cgaagaccgc
     1681 gcgggtgtcg cccatggtga actccaaacg cacgacttgc cgagcgtggc gctttccggc
     1741 aagaggtggg tcgacgtgta cacaactccg ggcttccagc gcggcgccgg cgtcacccct
     1801 gttctgattc tcttcgatgg cgaggaagcg aaagcgctgt cgaagacgcc aataattctc
     1861 gacaatctct tcgccgagcg gcgcataccg cagatgatcg cagtcttctt ggcgcaaccc
     1921 tacgagcggc gggagacgga cctggcgtgc agcgaggcga ccaatgtctt cctgaccaaa
     1981 gaactcctgc cttggctccg cggggagtac aggatccgca ccgcagccga ccggaccgtt
     2041 gtcgcaggtg cgagcttggg cggcctcgcg gctgcctacg ccgccttgcg gcatcccgag
     2101 aagatcggcc gcgtcctttc ccaatcgggt tcattctggt ggggaaggac ggatgcggag
     2161 cccgaatggc ttacgagcga gttcaagtcg aagccgcgag tgaatgcgag attctatctc
     2221 gacgtcggcc tgatggagac tgaagggaac gccatctccc agcttgaaac caaccggcgc
     2281 ctggtggcgg tactgcgctc gaagggctac aaggtcgttt atcacgaatt caacggcacc
     2341 cattcgtacc catgctggcg cgctggattt gcggatgcgc tcactgcgct gctttctgat
     2401 tgaatgcggc acgtggtcgt gcgtctttgg ttctaatcga tgaaccgcgg cgaatagagt
     2461 ggatcgccga cgaagaccat cttccagttg agctttggag tggcgaggta cgccgcctcg
     2521 ccgaaggtgt atccgttcca gagacggttg agcagcgcat cgcccacggt gtagccgtta
     2581 acgtacggct cgccggtcgc gccccaggtg gcggtgatgc cacgctccag ccagagaggc
     2641 acccaggcgc cgggtttctg gtagcggatc gactcggctg tgtatgaggt aagctgcgcg
     2701 ccgactgcgc cgggagcgaa cgagtacacg tcgttggtgg agaaactgta ccatccccac
     2761 gcccacagcg cgccgggcgc gctcgcgatc atgccgccgg tcgccgtctg gtcgttgagg
     2821 atacagtcga ggccggcgga gacgcacaat tcgtaccctc cgatcatcgt ctggtcggct
     2881 acataatatc cgccgctcga aggggacaag tggcgccaat cgaagtaacc gacacccgac
     2941 ctcctactga tgccactttc ggcggcgagt gccttgtcga ccagtccggc ggcgatttca
     3001 gcgctcggtc catccagccg cgacacgatg tagtaggggc cgccgtaggc gttgcgcgga
     3061 aaccggggca tgacctgcgg gtcggggctg taggccgggt tgtccatcgg gatgacgtct
     3121 gcgtaacggg agctcatgag ggtgagaaac gcatccacgg agaggccggt ggcgccgtac
     3181 gggtggcgct cgatgtgcga gggaacgcca tagaccggca cgatgtagtg gatcttgcgc
     3241 gcgagaccgc tcgccttgag aaaggccttc agcggctcac ggacctcgtt cacaaactcc
     3301 tgccaggtga tgttccaact gtcccacgct ctcgggtcgc tgtcgcagca ggccatcgtc
     3361 gtgcggatgt gggcgatatt gcccgccggg attccacggg cggccgcgta ccgggcggcg
     3421 acgtactggc ccgcgttgac gccttcagtg ccggcttccg gaggggtatt gtcgttaatg
     3481 acgattagca ccgaggatgg gtctgtggag ccgcccgcgc acgtcgcgat gaagggagct
     3541 ggcaggagcg ctcccccaac aatcagggtg gaaagcagcc gcgtcatctc aggttcacct
     3601 caaggagaaa tcgactcctt tgggtagaaa actcgtggga tttcccccta atccgtcaac
     3661 gggagaacct gactttcccc aacacgaacg ctcgctgggg agagggaaat gacggagaat
     3721 gcatgttggg atcgtggaaa ggacggatgg aagatcaaca tgggcgagac tgattccgcc
     3781 atcaacacac cggtggttcg cgtaggcatc gccggcgccg ggatggccgg cagtttccac
     3841 gcggagtgtc tgcggcgcgt tcacggcgtc cgggtgaaac ttgcgggagt gacgtcgctg
     3901 cgtccggaga gccgccgcga atttgggatg agccgcggca tcccggtgtt cgattcgctc
     3961 gaggagatga tcgagcacgt cgatgtggtc gacctttgct cgccgccgta cgcgcataag
     4021 gacgggattc tcagcgcagc ccgcgcgggc aagcacatca tttgcgaaaa gccgctgacc
     4081 ggattctttg gacccccggc cgacgacggt tttcgcggaa acgcatttcc aaaagagaag
     4141 atgctcgacg aggtgtgctc aagccttcgc gagatcgcct ccgcggtgtc ggaaggcggg
     4201 aaatgctttg gttatgcgga gaacttcgtc tatgcgcccg ccgtccagaa ggagcgtcag
     4261 attgttgaga aatccggggc gcagattctc cggatgcttg gcgaggaatc ccacaagggc
     4321 tcggcctctc cggtgtacgg aatctggcgg tatgccggcg gcggttcgct gatcggcaaa
     4381 ggctgccacc cgctgacggc gctgctctac ctcaagcgca gcgagggact ggcgcgcgac
     4441 ggttcgccca tccgtccggt gacggtcagc gcgcgaacgc acgaactcac tcgtctgccc
     4501 ggctatcggg accggaagtt tcttcggacg gattatctcg atatcgagga ctgcggctgg
     4561 atgcacgtgg tcttctccga cggtacgatt ggcgacgtgg tcaccggcga actcactctc
     4621 ggtgggatct acgattacgt ggaggtcttc gccaataacc accgtgcgcg ctgccgcctg
     4681 agcccgacga acctgatgga tgtctacaac cccagcggcc gggagttcga cggaatctac
     4741 ctgatggaaa agatcagcac gcaggaaggc tggtcgaacg cagcgccaga cgagaactgg
     4801 accatgggat accagccgga gattcaggat ttcatcgagt gcgccgctac cggacgcgag
     4861 ccgcagtcgg gcctcgcgct agcgctcgat acgacactga cgatctatgc cgcgtatgtg
     4921 tcagccgagc ggcgtggcgc cgaggtcgaa gttccactgc ttgccgagga gaccgctcgg
     4981 gcttgaaggc ccccatcccc cgggcggaag aacaggcgcg cctacgaaac acgaagctat
     5041 tcagaacgta taggaaagcc cgatcagcgg aaccaccttg tctttgaggc ctccgtcatc
     5101 gagccggaac agcaagtttc cggtaagcaa cagcgagccg gcgatattca acttggctcc
     5161 ggcggcagcg cttgtgatgt tgaacgactc cgtggcgaaa cgcgtgttcg cataggtaac
     5221 ttggcctgcc cccggattcg agagttccgg cgccggcgaa acaaaagtgg tcggcagcgc
     5281 ccgggcggcc ttgaaaaccc gttgtcccaa gagatcgaat gcaagcgtca gcggccgtgc
     5341 aacgccaacg tcggcgcctg ccgtatacgt gaactggttt gccagatgac cttttcggtt
     5401 cgccgaaatg tcgccggcca ggagcgaact ctggttgact tgataaccca cggtgaaatg
     5461 cggagccacc cggccaacgc gggcggagta tgcgataaaa ggtctcagcc cgaacgcgcc
     5521 cgaaccgaga aagttccgtt catcaccgct cggcacgtac aggtccgccg ccaccgcgaa
     5581 cgaagaggac tcccaacgtt tcactgtgcc cttcaaccgg atgattacat ctccaattcc
     5641 cgtcgccgag ccggagctgg taaacgtttt cgcaatgctt ccatcccggt tctgcgaatc
     5701 gaaatagtga gcctgtccaa acgtgggatt cggcggggca atcctgtgaa tctgcgcgtc
     5761 cgaagcgact ttcatcttca agtccagaat gggaatggcg accgacacat caatccggtt
     5821 cgtcaggcca accgtgccga atatcgtgaa ctggttgaga gtcatatcga ccgagttcct
     5881 ggcggtgacg aaatctttct cgtattctgc ccccgtttcc tgttcatgcg taaaaacggt
     5941 cggcatgctg tcaaggggga tgccgtcgat actgtcaaac gagaagcgct ggtacgcgaa
     6001 tccgaggaag aactttcccc ggccaatcgt ctcggcccgc tcggtcaaaa tagggccgaa
     6061 gctttgggcc gaccgagtga atacccccgt ggccgagtcg aagacatagg tgaaacccga
     6121 tgccggtgag gcgatgggca accgcgtcag ctcgctggca accgcgccgt tgaatggccg
     6181 gaaattcgac tggaagacac tgagaaagtg agcttcatgg aatggattcg gcaagacaaa
     6241 gccctccgaa ccgtacaggt tcggaatgag gcatcccaag tcactgctgt tcgcgcaagt
     6301 attgacctga gagtaagctt gcgtccccaa aagtgtgagg cagagagtga gagacgacac
     6361 caatcctttt cgcgccaaca tgtttccctc cgaggtgtta ccgagaggct agcagagcga
     6421 ccagaagcct gtcaatgtat tgattttagg gcctttatgt gcctgttagc ctgcttagtt
     6481 gaggggtgac aagctgacga tgcgccactc attcccctct tttttcaacc tgaacagcgc
     6541 aacatcattg gcggtctgct ccgcgccgcg gttatctttc accgtaagca tacgaacaca
     6601 gcggaccgta gccgtctcgc ccgcgacctg cggctcctcc ggtttcagtt gaaagctgat
     6661 tacctggaag acactaaagg acttgcggat ctggtcgaac tggttgttag gcatgttcac
     6721 atacaatgcc cgcacccctg cggcgtccct ccgcctgtag gcctcctcgt aacggctgac
     6781 cgtcgcccgg acggcagccc cctccgatcg ctgctgctgc tcctgttgaa gctgggcgat
     6841 ctgtttcctc gcttcgccca caaaagtgcc cctgggatgc tttgccagga acgcctcgag
     6901 tgacgcgaga ttatttctgt tggttgccga ccactccagt tcctcgatcc tcgcctccgc
     6961 ctcccgagca aaccgccctc cgggatagcg ggtgaggaac tccgccaagt ccgagatgtc
     7021 acggctgtcc ctgacgtgct gccattgctg cgcctcaagc tcagccggac tgggctgtgg
     7081 tggaggcggc gggcttacgg ccttaaccgg tgctggctcc ggtgccggcc ttggaatcgg
     7141 ctggagccga agctggtctg tgccaagctg gaggcgctcg ccgggagaga aagagcggct
     7201 gatggcggcg ggttggtagc catctttgct cagctcgatc cgccgctcgc ccggctgcac
     7261 ttgtaccgag aaaactccgt tcccgcccac agcgccggca agctttcggt ccacggcgac
     7321 ggttgcgccg ggaacttggc cggtgattgt cagaaccgcc tccagaggct ctaaccggaa
     7381 aggtgcgcgt acctgctcgc ctttccgcac cgccgctcga acaacgggtg gaacgacacg
     7441 atagccttgc ttttcaacac gaacttcgta ttctttcgcg tcaagtggga tgctaagttc
     7501 tccccgttct gtccggcgag gccactgctt gccgccaatg tagacgactg cgtcatccag
     7561 cccggtctga acgagcagga tcccgcggtc ccgagcggaa gtgagataag tgaccagcga
     7621 cggctgggtg ccgaccgcga acacaagctt acgcggagcc gctccgccgc cgacctgaag
     7681 ctcgtgcgtt ccggctgaaa cgcccttgag tgtcagttga ccgcccacga agcggcccac
     7741 cagcgtgccg tccaatacgg cttctccgtt gcacctcgcg cagttcacag ccacctcatt
     7801 gccaaacgcc gtcacaacga cggcgtctgc atctcgcgtc gatggtggcg aactgagcag
     7861 cggccgcttc gcctcggcgg tttcaaaagt cagcttgaca tcaccgtctc cgcccttcgc
     7921 ctccaattcg tgccgtcccg ggccaaggtt gtcggcgatg aactgtccgt ctcgtaaccg
     7981 gccggccgtt cgtccgtcaa gcgtgacgct cccgtcagcg aaggtggttg taagttgcac
     8041 ggtggagggg agcgccgata gcgcgatttc aaggctcaca ggggcagagt tcttgacgac
     8101 ggtggtcttc ctcgtttcgg ttcgatagcc cggcaattgc acctcgaacg cgtaatcacc
     8161 gggcgccaac tgaagacggc aatcgggtgg agtgcagctc ccgtccttgt aacggaccac
     8221 tgcgccggcg ggggaggttc gaacctcgac tggaatagta ccgcccggcg gggcgttgat
     8281 ctgtcgtata acaagaaccg cacctacgcc tgctaccaac acggcagcca ccgctatcgc
     8341 gcggcgtggt gtgatcaggg gtgagcgctc ggcaagcttg ggctccaact gctgcgggac
     8401 ttcctgtggt ggtcgcgcct cgtcaagctc acggcctgcg cgcgccgttt ggatctgatt
     8461 ccgcagctcg aggagtcgcg cctcctgcgg atgctttttg agggctgcct tgagcgactt
     8521 ccgcgccttg tcgaattccc cggcttccac cagcgcctgg acctcacgca cagcgtgctc
     8581 aatcgcctcg gctttctgct tctgctttaa cgcttggcgc gcctgtctca aaacttgctc
     8641 gagttgcgcc tctccggtga actccactga cgccacttcc aggacgcgga tcgcctcctc
     8701 aggctttcca gcttggacga gcgcttctcc gttagccaca gccgtttgta cagcggcaac
     8761 ccggcgccgt tcctcgcgcc cctttgcgat ccgctcagtc agcggtgcaa gcgcagggtc
     8821 gtcaccgtgc tcccgccgga cagcggcgat gacggcgacc gccctctcga attcgccggc
     8881 ctcaaccagc gactgaactt cccgctcagc gcgttcaacc gcctcggctt tctgcttctt
     8941 ctgtagcgct tcccgcgcct gtctcaaagc ttgctcgagt tgtgcctctc cggcaaactc
     9001 cgctgacgcc ggttccagga cgcggatcgc ttcttcaggt tttcccgctt ggattagcgc
     9061 ctccgcgttc gccgcagccg tctggacggc gactgcgcgg cgccgccgct ctctaacgct
     9121 ttcaatccgc tccgtgaggc gtgccagcgc cgggtcgcca ccatgctgtc gccgcacggc
     9181 ctctatgaca tcgagcgcct tgtcgaattt ctcagcctcc accagcgcat gcacttcacg
     9241 ctcggctcgc tcaaccgcct cggctttctg cttcttctgt agcgcttctc gcgcccgtct
     9301 caaaacttgc tcgagttgcg cctctccggt gaactccgct gacgccggtt ccaggacgcg
     9361 gatcgcttct tcaggttttc ccgcttggat tagcgcctcc gcgttcgccg cagccgtctg
     9421 cacggcgact gcgcggtgcc gctcctctcg tgccctcccg atccgctctg tgagttctgc
     9481 cagccccggg tcgccaccgt gctgtcgccg cgcggcctct atgacatcga gcgccttgtc
     9541 gaatttctca gcctccacca gcgcatgcac ttcacgctcg gctcgctcaa ccgcctcggc
     9601 tttctgcctc ttctgtagcg cttcccgcgc ctgtctcaaa acttgctcga gttgcgcctc
     9661 tccggtgaac tttgctgaca ccggttccag aacctggact gcttcctcgg gtcgtccggc
     9721 ctccaaccgg gttttcgctt cggcgagagc ggatcgaatg gacgcgattc gctcctgccc
     9781 cgccttaacc tcctccagga gttgcagcag cctggcgtct ccggcaaagg accgcaaggc
     9841 gtcttcgatg acgccgagtg cgcgtccata ctgatccgat tcaacaaagc tccgcgcttc
     9901 ccggacaaga acttcgattt cagccgcgcg cttctgggca ctgagttcct tctcagcaag
     9961 cgccagaagc cggccgatat cctcgttctc cggaacttcg cgcctcagtg tgctcaagcg
    10021 ctcgaacgcc tgcaagaacg ccgaattccg cagctcttcc gtggcagcgg cgagctccgt
    10081 tcgtattcgc tcagcctgct ctcgctcggc ccgttcgaac tccgcgcgcc gtgtgttgag
    10141 cgactcgatc agtgctttgg cttcggcgga gtcgctttgt tccgagtcca attcggcgag
    10201 cgcggcggca gcctggtcga agaggttttg atcgagcaat tcctgcgccg cgccaagcac
    10261 ctgctggaac cgtaaacggc gctcctcgcg ttcagccgcg gtctgaacgc tctgaacgaa
    10321 gcgcgcgaca tcagtgcatt ccgggtcgat ttgcgcggcc tcggacgcga ttcgaagtgc
    10381 cgcactgtag cctttctgaa aaaaatgctt gcgggcttcg gccagcagtt gtgccgctgc
    10441 gcggctccgt tcgagcaact cgcgcgcctg ctggagccgg cccttcaact tttcgttttc
    10501 cgggtacact tgcagcgcgg attggaaacg ttccgctgct tcggcgaacc ggcgttgctt
    10561 gagcgcatgg tcgccttccg ccaagatggc atcgatgcgc gattgaagac gccgggattg
    10621 gagccgctgc tgcacttcgt tccgaaactt ccggcccgcc gtattggacg gatcgagagc
    10681 gagcgcttcc atcacgtgcg cctcagcccg ctccaagtca tcctcggcga agcagttgcg
    10741 ggcatcgtcc aagagaacgg atgcccgttt ctgccgaagt tcgagcagga tcggttccgt
    10801 gtcgatctgc agttcacgaa ggctctggta gcgtaactcg cgctcccgat gtaaagcgcg
    10861 gcggaccact tgctcaaggg cttcggggca ctctggagtg tggttgcgga tcggctcggg
    10921 atcgactgaa gtgatcttgt aaagcaccgc tccagcgtcg gcggcgtgaa aggggtgctt
    10981 cccggtcagt agctcgtagt aggtgactcc ataggcaaag atgtcgctga gggcgtcgac
    11041 gtcggcgctc atgatctgct cgggagacat atagagcagc gtcccgatca cgtcgccctg
    11101 gcgagtgagg cggggcgacg tgctatcccg cgtcagccga gcgatgccaa aatccaggat
    11161 tttgacagtt ccgtctggca gcagccggat gttggcgggc ttgacatcgc gatggacgac
    11221 accattacga tgcgcggagt acaggccttc ggcgacctgc atcatgatgt tcaccttctg
    11281 cagcatcgtc agtggagtac ggttcgcaat gatctggctg agatcgtcgc ccgcgaggaa
    11341 ctccatcgcg atgtacggca tcccatcgtg ttcgccgaag tcgtaaatgg tgacgatatt
    11401 ccggtggttc aggctgccag ccgctgaggc ctcgtttcgg aagcgcttca gcagatctgt
    11461 gccttctccg gttaagacct tgattgcgac gagccgtccg acagtgggat cataagcgcg
    11521 gtagacccgg ccgtagcccc cgcggccaag ttccgcctgg atctcgtagc gcccaatatg
    11581 cttgattgag gcctctgcca tggggaaact agcccggtcg gcgacgatga tcccagccca
    11641 gggaatatct aagataatac cagcacaaca atagtcactc aactttttcc acttcagtag
    11701 tatggggtgt cgtctcaacg gttcggctgg cgatcgtgct cactaccctc aactgccgtg
    11761 cgcagcgatc cgaaactgct tgcagaatcc gttggatcgt ggtttcatta ctagatagag
    11821 cgtggctggg cgacaagacg ctctgggata acagttctca tcaattcggg catgatctct
    11881 gcgtgtggtc tcagcgacac tggttgtgtg cgcactgaca accaggaccg gttattggtg
    11941 gacgatgaac tggggttcta cgcaatcgct gatggtatgg gtggccacag ccatggcgaa
    12001 gtcgccgcgg agctggcgat ctcgaccatg aagtactacc tggagtcgtc gcacgatcgg
    12061 gccgacatta gctggccctt cgggtttgat ttcgatctgt ccgttgattc aaaccgcctc
    12121 attacggcga ttcgtctggc caaccaccag gtgtggaagc gggcggaaga aaaacccgaa
    12181 tacgccggaa tggggacaac ggtagcggcc ctgctgattc atgacaatca ggccgcggta
    12241 gcgaatgtcg gcgacagccg cgtttacctt tttcgcaacg gcgacctcaa acagatcaca
    12301 acggacgaca cttggctcaa cgccatcctt cagcgcgggc cgaactacgc tcagctccga
    12361 agccatcctc tgcgcaacat gctcacacag gcggccgggt cccaatccga tgtttcggtc
    12421 cacaatgccg ccctcgaagt acaagcaggg gatcgccttc tgttgtccag cgatggcctc
    12481 catggagtcg tcgaagacga agagatacgc ggcattcttg ccacgggcgg ttccatcgag
    12541 gaactggccg cgcggctgct gggagccgcc cgaacacagg gtgccccgga taatgtttcc
    12601 tgcattttgc tgagttttgg agacaggtga ccggcgtggg cgagcttttc ggcagatatg
    12661 aaatcatcga ggagatcggc agaggggcca tggggacagt gcatcgtgcg cacgacacgg
    12721 tgctggacag agcagtcgcg atcaagagga tgcgcagcgg cccgcgcgtc gatccggagc
    12781 tgacgaagcg gttttacagg gaagcgcaag tcggtgcacg cttacagcat tcgagcatcg
    12841 tgaccgtgta cgacctcgga gagcacgagg ggacttcgtt catcgtcatg gaactgctcg
    12901 acggaatcga ttggcgcaag gtcatcatcg agaggcgcgc catgcctgct gcgaaaagga
    12961 ttgaattgat ggcgcaggtc tgcgatggtt tggctcacgc ccaccggcac acgatcgtgc
    13021 atcgggacct caagcccagc aacctgttca tacaccagga ccatcaggca aagatcctcg
    13081 atttcgggct cgcgcgcatg cccacttcct ctctgaccct gacgggcagg gtgttgggaa
    13141 caccaaacta tatggctccg gaacagatac ttggccagcg cgtcgattcg agatcggatc
    13201 tgttttcggc cgcgattgtg ttcttcgagt ttctgacggg aatacacccg ttccgcgggc
    13261 ccattgtgcc gcggcagatc gtggacggtg agcccgaacg cttagtcaac ataaacccat
    13321 tgctgccgga ctcattgggg ctgatcttcg aaagagccct tgccaaatcg ccggatgcaa
    13381 ggtatcaaga cgcggcggaa ctggcctcag acctgcgtgc ggcagttcgc gaggttccgg
    13441 aagagcctgg cgctcaagtg ccgctttcgc ctgaaagttc cagcctgagt ccggaaagtc
    13501 aaacacaggt ctctgcgcct gacgctccct tgtatgcggc tgacgccgac accgagacca
    13561 tcgtgtacct cgcacccgag acgagaccgg tgcctccggg acccaacgaa ggcagctcgc
    13621 acaagtagtt ggatcaggac ggctttggcg attcgtcaag gaacgctttg atgccttcca
    13681 acgaagcagc ccagactctc acgggtgcgt cgtatccctt aagcctggca tcgtgcggct
    13741 gaaaatggtg ttctcgcaca ggactgaagt gtagcgcttc gaaggtagaa gcgccgatga
    13801 cgacatccgc gccgatgtct ttggtagcgg actcaatgcg gaacgctgcg ttgacggcgt
    13861 ccccgagggc cgtgtaatcc gtaaatgtgc gcgtgcctgt attcccgacc gaagcgtaac
    13921 cggtattgat gccggcgccg atccggattg gcgccggcag ctgaaagtcc gcctccagcg
    13981 cagacgtcgc ttcagcgaat tcgacgagtg cttgcagaat ctgcgccagg tcgcggctcg
    14041 ctgcccccgg ctcactgtgc agccacgtag acataatggc gtcgccgatg tacttgagag
    14101 cccacgttcc gtgccgcttc ataatctcgc tcgcatggcg gaaccacgtg ccaatggcct
    14161 cgctcaacac gctctgttcc gtgcgctgtg tcaggcgggt gaaatcgcga atgtccacga
    14221 ctagaaccgt aagaaactgc cgctgaaagc aggcttccgt ggcggctgtt tccgccatca
    14281 cgctgcttgc cgggtccgag cccccgccgg gccagtgaaa cgtaaaaaca aatccgccga
    14341 tagacactcg gtcgccgtcc ctcaggagcg caggggtgct gacacgccgg tcgttgacga
    14401 aagacccgtt ctgactaccc atgtcgatga gatagtaatc gcccgagtcg tcgcgctgaa
    14461 tgatcgcgtg atcgcgggaa acacgcttgc tcgcgagggc gatatcacac gcgtcgttgc
    14521 ggccgattct ccacatatag ccgctcatca gggggaactc acgctcaccc cccgcatccg
    14581 gcacaacaag gtaagggaac ccagatccca gcaagatact ctctcccctc caccctatta
    14641 tgtacccgtg cgcgattcga tgcgaaagcg cttggggaac tgacgcccat ggcctcgacc
    14701 tgcctggtga ttcagccgag cggcgtggcg ccgaggtcga agttccgctg ctttccgaga
    14761 agaccgcgcg ggtttgaagg cccccagtcc cccgggcggc ttcagcacgc tctcactctc
    14821 aaaggcagac gtgccagcct tccgttgcgc cgagcgtgtt gtcgaagaac aggcgcgcct
    14881 gctggacgat ttcgaagacc gtttgatcgt cgtgccatgg gtcgtggtgg aacaggatca
    14941 gcgtcttgac ccttgcctcg tgggcgacac gggtcgcctc cagccaggtg ctatgacccc
    15001 atcctttatg gctctcgtat tcctcggggg tgaactgggc atcataaacg aggacgtcag
    15061 ctccctcggc gtactggcgg agcacattgt cgagctgcgg atggccgtgc tcgaggtcgc
    15121 tcgcgtgtac gatcaccttc tctcccgcct caatcctgta tcccaccgct ccctggggat
    15181 gattcaacgg aaacggatgg atcacgaggt ccgcaagctt gaagggtttg tcgtccagtt
    15241 ccgcgaactg ccgccgcgcc gccagaacct cgaaagcaat cgggaaatag ggcctcgaca
    15301 tctgtccctg caacttctcc tcgatcgacc ccaaagcttg tcccgtgtag aacgtcgcgt
    15361 cggtttccgg tcggtacagc ggcgcgaaga acgggatacc ttgaatgtgg tcccaatgaa
    15421 agtgcgtcaa gaggaaatgg acggacaacc ggctgtttcc ggattcctga agaagcgtct
    15481 ggccaagttt tcgcaggccg cttcccgcat cgagtataac cacctggtcg tcaggtgacc
    15541 ggatttcgat gcaggcggta tttccgccgt aactcaggtt gtcgatttgg ggggtgggcg
    15601 ttgaccctct gaccccccag aacttcagcc gcagaccgcc gttgccgccc attaggtacc
    15661 ctcttcgccg aatccgccgg ttcgccacaa cccagtttga cttactcctg gccctgccgg
    15721 ctcgctaccc agataattcc cgaattatct cacaaatagc acagcattac atcacttaat
    15781 gtcaaaataa ccaccgtaag gaccggggtg cggcgatctc agatggtgga ggaacctccg
    15841 atgaggcgct gaaagatgga gcgcttcttt ttgtcaacgc cggccacggg cggcggcgcg
    15901 acaggcacgc agcgctgagg gtgttgtgaa ttcccgaagt cggggctata caggttataa
    15961 atctcgacgg tgactccgtg cttcacctgc tggatcccga gctcgtggag attctgccgc
    16021 cattcggaaa gttcactgag aatttcggcg atagccttgg agacgaggat gtggtccgcg
    16081 tccgcgcagt ccattactct ttgcgccaga ttgatgcccc cgccggcaac attccgggct
    16141 ttgttgatgt ccaccgttcg ataaacgggt cctgcgtgcg cccccatgcg gagcctgatg
    16201 aacttgtgat ttttgagcgc tcgcgcgatc tccaccgcgc actcgaccgg tgacataggg
    16261 ctatcgaaaa agacgagcgc cattccgtcg cccgttggaa taagaacgat ctgatcgctc
    16321 cggctcgccc gcaggtaggg ctgcgttgag agcacaacgg aagacaggtc ttcgaccgcg
    16381 cgcgcctggg tgtccatcag cagcttcgaa taacccacga tgtccagaaa caaaacatag
    16441 gccatcccga gcaccggctg cgacggcgct tcaaacgccg gcgccatggt agcggtatcg
    16501 acgccccgga cggctactgg aatcgcgttc ttccgggaag tagccgccgc caggtccgcc
    16561 agcagatcgt tgacttccgc gtaccggtcg tcgggctgct tggccaacgc cttctgcgcg
    16621 atgcgctcga gctccggcgt agcttccggc cggaggctcg acattggcgg cggatcatct
    16681 tccatgatgg cgaacgtgag cgagacaaaa ttgtcggcct cgaacggtag ccggccggtg
    16741 atcatcaggt acatcaggat gccgagagac caaacgtcgg cgcgctgatc gatccttccg
    16801 cccttgagct gctctggcgc catgtatgct ggtgttccta gcacgacgcc cgcctgcgtg
    16861 agcgcggagg gtgccgcctg aagtttcgcc aagccaaagt cgagaatctt caccgaccct
    16921 tcgcgagtca ggagcacgtt accgggcttg atgttcctgt ggatgattcc tttcagatgg
    16981 acttgcgcta ggccgcggcc gatctgactg atgatgtcga cggcctgctc taaaggcaaa
    17041 ggtccctgtt taatcttctc cgagagcaga acgccttcgt agtgcgcaaa cgacgtgaac
    17101 aattgtccat cgtcagtctg ctccatcgaa tagaacgtgc ctatgtttgg gtgatccagc
    17161 gccgaggcgg cctttgcctc cagaaggaac cgggccttcg cggtttcgtc gcgtgcgacc
    17221 tcccgttgga ggaacttgag cgtaacaatt cgatccaggt tcaggtcctg ggctttgtag
    17281 attggcccca cacgaccatc gccgagtttt tcgatgatct tgtagtgctt gatggtctgt
    17341 ccgatcatga cgtcgtgccg gttaattata tcgcaagtag gagccgatag tgtcatgatt
    17401 gcgaaacgag aaaagaacct gtaactaaga aataatttca gagtcaattc accggtaaca
    17461 atgtcctggc accggcgcat gaccaggtta catcggaggt gacggcggcg gaaaaaggaa
    17521 gtagactgga aggccgggat gccggccacc ttctggtacg tccgcggcgg atcctccgcg
    17581 ggcgcggaat ccgggcgtcc caactcaagt tcgagcgctg aggagctggc gaatgtcctt
    17641 catcgtatcc gaccccgtgg cctggctttc atccatccgg ctgcacggag ttctcgaaga
    17701 gataggcctg tccatcatca ttgcctccgc cgtcggttac gtggcccatc ggttgaaaca
    17761 gcccatcatc ctcgcctatc tgctggccgg gtgtctgctc ggcgagaaac tgggggttgg
    17821 acttctcaag aacccggaga gcatcgaact gatctctgag atcggcctgg tgctgttgct
    17881 gttcgtgatc gggctggaac tggatctcac gaagatcatg gtttccggca gacagttgct
    17941 ggttgcgggg ttgggtcagt tcgtggtctg cgtggcgctt ggactgggtt tctttggact
    18001 ctggggcctc tcgccaagcc aggactggct cgcggtgttg tacctttcgc tgctcgcctc
    18061 tctcagcagc accgcgattg ttgtcaagtt gctgtatgac aagtttgagc tcgacacgct
    18121 tccgggacgc ctgacgctcg gcatcctcgt tttccaggac gtatgggcga ttctgatcct
    18181 ggcgttccag ccaaacttcc aggatccgcg gatcgccatc atcggaagca ccgtgctcaa
    18241 gggcgtggtg ttgttcgtgg tgggcttctt cgtcagtaag tacctcctgc gctttgtctt
    18301 cgagtcggtg gccaagtcgc cggagatggt agtcacgctc agcattggtt ggtgcgccgc
    18361 cgtcgcgggc gtggcccagg ctttgggcct ttctaaggag atgggagggt tggttgccgg
    18421 cttcgccatc tcgaacttcc catacggaat ccacgtttcg gcgaaggtct tgcccctgcg
    18481 ggacttcttc ctcacgctgt tcttcatttc gctcgggatc aagatcccga ctcttgagcc
    18541 gcggcttgtc ccggccctcg gaggtctcgt cctgtttgtc gtcctctcgc ggtttctttc
    18601 cgtgtacccg ctgctgtcgg tggccggcag tggccgccgg acctgtttca tcaccagcct
    18661 caacctctcg caggtctccg agttctctct ggtgatcgcc gcactggggg tcaactacaa
    18721 gcacatcaac gacgacgtaa tgacgctcgt tctttacgcc ctggcgatca cctcggtggt
    18781 gtcctcgtac acgatcaagt acagccagcc gttgttcgtg ctctacgacc gtgcactgaa
    18841 ccggatcggc tggaggcaaa agggcacgga ggcgccggag gtcgccgcgg ctggccacgg
    18901 acagcactcg attgtcctgc tgggctttca tcgggccgcg cgagcttttg tcgagagcct
    18961 cagtcgggct ggcagcccgc ttttgaagga attactggtc gtcgatttca acccggaggt
    19021 cctcagcgag atgagagcgc ggaacgtcgc cgctatcttc ggtgacatcg gaagcccgga
    19081 cacactgctc cacgtccacc tcgaccaggc ccgtgtgatc ttgagcacca ttcccgacat
    19141 gctcctgaag gggacgagca acctgcagtt ggcccgcgta tgccggtcac tgtcgccgaa
    19201 cgcaagaatc gtgagcgtgg cggactccgg cgcccaaatc caggacctta tcgacgcggg
    19261 cgcggatgac gtcatcctgc cgtatgatct gcacggggac cggttggccg aaatggttca
    19321 gacactgggt tctgagcaga agttcgacca tgctcgcaat tgatcggatt ttcggcgcgc
    19381 ctcggctgca cttagcggtt tgtggaaagc cgttcaatag acgtactaga ccagccgtga
    19441 cacgtcatcg gcaattattg ctttgcaaag cttgacaact gtgatagatt atacctacct
    19501 gtctaacgcg aaatctggca tcactgggaa gcaaagcgtt ttgcgttaga actgtctggg
    19561 agctgcagaa acaggagtgg ttgcagcact gggatctcga tcgggttcac attgagaggg
    19621 gacacatggg gagcagaaga gactttctga aggctagtgt tggcgcgatc gcaggagctg
    19681 gggcgttttg gtcgtcgttg cgagtcgcaa aggggcaatt tccacctccg ccgccgactt
    19741 ctcctccact tgagcctttc gtcgatcctt tgccgctgcc gcccgtcttg cggccggtcc
    19801 gcccggggtt ttggttgggg cgctggtggg ggctcgcccg ggctctggga cctcactacg
    19861 agctgccgat gctggaggtg agtcagaagc tgcaccgaga ccttcctccg actcgcgttt
    19921 gggggtatgg cggaatgtat c
//
LOCUS       DATIFQ010000147         3558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1391031, whole genome shotgun sequence.
ACCESSION   DATIFQ010000147 DATIFQ010000000
VERSION     DATIFQ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391031"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VLE22_06995"
     CDS             complement(<1..536)
                     /locus_tag="VLE22_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSB14189.1"
                     /translation="MVTRYGGPEELRVVEEECPEPKNGEVRVRVLAAGVSLPDVMARE
                     GVHPETPPVPFTPGWDLVGVVERLGDGVSGIEPGRIVAALPIHGAYAEFVCLPQRELV
                     PVPSGLDAAEAVSLVLNYITAYQMLHRSARVRQGQRVLIHGAAGGVGTALLQLGRLAG
                     LEMYGTCSSRGTTAVSGLG"
     gene            complement(698..1252)
                     /locus_tag="VLE22_07000"
     CDS             complement(698..1252)
                     /locus_tag="VLE22_07000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSB14190.1"
                     /translation="MKPYRIAVVVGSLRRDSINRKLAKALVKLAPSDFSFEFLNIDDL
                     PLYNQDDDANQAASVKRLKGEITAAKGLLFVTPEYNRSIPGVLKNAIDNASRPYGQNA
                     WAGKPAGVLGASIGQIGSALAQQHLRNVLAYLDVPTLGQPEVFLHVKDGFFDEAGDIT
                     NTGTIKFLQEWMNRYVAWVKMHTA"
     gene            1619..2548
                     /locus_tag="VLE22_07005"
                     /pseudo
     CDS             1619..2548
                     /locus_tag="VLE22_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            2607..3218
                     /locus_tag="VLE22_07010"
     CDS             2607..3218
                     /locus_tag="VLE22_07010"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HSB14191.1"
                     /translation="MGGKLGTLFARAGHEVVFSYARRTEKLKRLAKDAKRNARAGTPR
                     EAAQDADALLLAVHWSRIDDVLRQTGDLSGKVIVTCSLPMNAGNTGLVIGHTSSGAEA
                     LGKRVPKARVVCAFNTVPSEVLFGVYEARRKANRPSLVYCGDDARSKEVAAELIRDVG
                     FDPVDAGPLRIARYTEPFALLMAQLAYEGNEGPELVYRFDRYG"
BASE COUNT          704 a         1075 c         1110 g          669 t
ORIGIN      
        1 cccaggccgg atacggccgt tgtccctcgc gatgaacagg taccgtacat ctccagcccg
       61 gcgaggcgcc caagctgcaa tagtgccgtg ccgaccccgc ccgccgcgcc gtgaatcaac
      121 acgcgctggc cctgtctgac cctggcggaa cgatgcagca tctggtacgc cgtgatgtag
      181 ttcagcacga ggctgacggc ctcggcggcg tccaacccgg acggcacagg aaccagttca
      241 cgttggggca ggcagacgaa ctctgcgtac gcaccgtgga tcggcagcgc ggcaacgatc
      301 cggcctggtt cgattccaga gacaccgtcg ccgagccgtt ccaccacgcc aaccagatcc
      361 caccccggcg tgaagggcac cgggggcgtc tcgggatgaa cgccctcgcg cgccattacg
      421 tcgggcaagg agacgcctgc ggccagcact ctcacccgta cctcaccgtt cttcggctcg
      481 gggcactctt cttcaaccac ccgaagttcc tcgggaccgc cgtagcgagt gaccataatg
      541 cgcgtgtgta tgatggacct cctcctctct tgccggccac tcgagctcgt gcggcgtaac
      601 gctcaggtca agctgcgggg gctcaggcct tcctttggtg ccgaaggggg gacccgaacc
      661 cccgcgggtt tccccaccag cccccggaag cttccgatca agccgtgtgc atctttaccc
      721 acgcgacgta gcggttcatc cattcctgaa ggaattttat cgtacccgtg ttggtgatat
      781 cgccggcctc atcgaaaaac ccgtccttga catggaggaa cacttccggc tggccaagcg
      841 tcggcacatc caggtaggcg aggacgtttc gcaggtgctg ttgcgccaag gccgagccga
      901 tctggccgat ggatgcgccc aaaacacctg cgggcttgcc cgcccaggca ttttgaccgt
      961 aggggcggga agcgttgtcg atcgcgttct ttagcacgcc ggggatcgag cggttgtatt
     1021 cgggagtgac gaagagcagg cccttggccg ccgtaatctc tcctttcagc cgtttgacgg
     1081 atgcggcctg gttagcatcg tcgtcctgat tgtagagcgg caaatcgtct atattcagga
     1141 actcgaagga gaagtcggac ggcgccagtt tgacgagggc ctttgcaagt ttccgattga
     1201 tcgaatcgcg acgcaggctg ccgacgacca cggcgatacg gtatggtttc atgacgcttg
     1261 acctcccgat cggttaatta ggctccccgg aacggttcct tgcgatgatc ccccaatttc
     1321 atcccgatgc ttggagcccg cgagctccat ctgagccaca tttccgattt tacgaaaaaa
     1381 gggacttcgg cgagaagccg aaatccctat gagtactgcg atggtgtcga agggggggct
     1441 cgaaccccca cgggtttccc caccaccccc tcaagccgcc ctacccctca ggagggatcg
     1501 ccccaatttc cttggacccc tcgaaaacga aagggctctc gtaaccgact tgaaacgttg
     1561 cggttgcggc atcgatctga tcatcgagaa ggtggagaac gggtcgaagg agaatacgat
     1621 gcgaaagcga aaattgggaa aaagcggtct ggaagtttcg gctctcgggt tgggctgcat
     1681 ggggatgagc ttctcttacg gtccgcccgc agacaagaag gagatgattt cccttctccg
     1741 aacagccgtg gaacgcggtg taacgttctt cgatacggca gaggtgtatg gccccttcac
     1801 gaacgaggag ctggtcggtg aggcgctttc ccctctgcgc gaccaggtgg taattgccac
     1861 caagttcggt ttcaacctcg atccggggtc ggatcccagg ggggtacagg gggcgcccgc
     1921 cctgaatagc cggccagagc acatcaagca ggtcgctgat gcctccctca agcggctcaa
     1981 gatcgatgcc atcgatctgt tctatcaaca ccgggttgac ccggacgtgc caatcgaaga
     2041 tgcagcggga gcggtgaagg accttatcaa ggaaggcaag gtcaagcact tcggcctctc
     2101 cgaagcaggc gtgtagacga tccgccgctc acacgccgtc cagccggtca cggcgctcca
     2161 aagtgaatac tcgttgtgga cgagaactcc tgaagcggag gtgttgccga ccgtggagga
     2221 actcggaatc ggcttcgttc cctacagccc cctcggcaag ggcttcctca cgggcaagat
     2281 cgaccaaaac accaagttcg acggcaccga tttccgcaac acgctgccgc gcttcacgcc
     2341 ggaggcgcgt aaagccaatc aggcactggt ggatttgctg ggtaatatcg ggcgacagaa
     2401 gaaggcgacg ccggcccaga tcgcgctcgc gtggctgctg gcgcagaagc cgtggatcgt
     2461 tcccatcccc ggcaccacga agcttgcgcg cctggaggag aacatcggcg ccgccgccat
     2521 agaacttacg gcggacgatc tccgcgggat cgagagcgct gcctcgatga tcacggtgca
     2581 aggggcgcgc tcttggctcg gggttgatgg gaggcaagct cgggacgctg ttcgcgcgcg
     2641 cagggcacga ggtggtgttc agctatgcgc gccgcacgga gaagctgaag cggctcgcga
     2701 aggatgcaaa aaggaatgcg cgcgcgggca caccgcgtga ggccgcgcag gacgcggacg
     2761 ctcttctgct cgccgtgcat tggtcgcgca tcgacgacgt gttgcgtcag acaggcgacc
     2821 tctcgggcaa ggtgatcgta acctgctcgc tgccaatgaa tgccggcaat accggccttg
     2881 tgatcggcca tacctcctcg ggcgccgagg cgctgggaaa aagggtgccg aaggctcgcg
     2941 tcgtttgtgc gttcaacacc gtaccgagcg aagtgctttt cggcgtctac gaggctcggc
     3001 gcaaagccaa tagacccagc cttgtgtact gcggagacga tgcgcgcagc aaagaggttg
     3061 ccgccgagct catccgcgac gtgggtttcg atcctgttga cgccggaccc ttgcgaatcg
     3121 cccgttacac ggagccattc gcgttgctca tggcgcagct cgcgtacgag gggaatgaag
     3181 gcccggaact tgtgtaccgg ttcgaccggt acgggtagtg agcgtcatgt ccgagctcac
     3241 cgaatgggac agtttccatg taatcgtcgg ctcggcctcg ggcgcgctga tcggtctgca
     3301 gttcgttggc gggatgacat cttacattgt gtacggcgga tcggcctcct ccgggaatcg
     3361 tccatatgga caagttgggc gggtgtttcg aagaagcaag gctgacgttt ggagaccaaa
     3421 tttcccgaag cctgaaacca caggcaaagg ccgtgctcac cgttctcaac gcatggagct
     3481 tcttctgctc tttcgccccg ccggcatgtc agtaatcaac ctgtgggggg gaaccgcagg
     3541 gaactggggg agaaggtc
//
LOCUS       DATIFQ010000148         2311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_625636, whole genome shotgun sequence.
ACCESSION   DATIFQ010000148 DATIFQ010000000
VERSION     DATIFQ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625636"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VLE22_07015"
     CDS             <1..186
                     /locus_tag="VLE22_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HSB14192.1"
                     /translation="SIAHGSLLEAETQLVLSGRLGYLSTPVVDTLLGKSAEVGRLVNG
                     LYASLESKEQGSGQASH"
     gene            208..1629
                     /locus_tag="VLE22_07020"
     CDS             208..1629
                     /locus_tag="VLE22_07020"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB14193.1"
                     /translation="MSIVTDRAAPPVSSPTARILVVDDERSMRELLAIVLKREGHEVL
                     VAEDGRSAIALLEKEPIELLISDVRMPDVSGVQVLAAAQRVNPDIIGIMVTAFASTET
                     AVEALRLGAYDYLSKPFNVDELKLKVRKALERRSLQQENQLLKRALQSTHQFSNIIGR
                     SSAMQAVFQLIQTIAPTMSTVLVSGESGTGKELVARAIHFNSPRKDRPFVALNCGALP
                     ETLLESELFGHMRGAFTGADTNKKGLVEVAEKGTIFLDEIGEMSPMMQVKLLRVLQER
                     KFRRLGGTEEIDADIRIIAATNRDLEKLVAEGRFREDLFYRINVIPVRLPPLRNRADD
                     IPLLADHFLVKYKGQARRKVSGISPDAMRLLQAYAWPGNIRELENAIERAVALETGPM
                     VQPSSLPEHVQTGVVGQGAGNGLEAPLPDSGFDLEQHVADIEREYIAEALRRAGGVKS
                     RAADLLGMSFRSFRYYMKKYNLG"
     gene            1745..2272
                     /locus_tag="VLE22_07025"
     CDS             1745..2272
                     /locus_tag="VLE22_07025"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSB14194.1"
                     /translation="MRNSRGFTLAELLIVVAVIGILAAIAVPALLRARQNANEASAVS
                     TMRTISSAEVTFAASCGRGGYAQSLADLSRPAPSGLTFIGPDLSQDPSMKSGYEIVLS
                     AEAGSASVTPAAGTCNGSANDAVRAFWAGARPLQLGATGQRAFAIDVRGAIFEAANGA
                     VHIANPVPPGTTVLK"
BASE COUNT          436 a          735 c          729 g          411 t
ORIGIN      
        1 tcaattgcac acgggtcatt gctcgaggcc gaaacgcagc tcgtgttgtc aggccgtctc
       61 ggatatcttt ccacgcccgt tgtagacacg ttgctcggaa aatcagccga agtgggacgg
      121 ctcgtgaacg ggctgtatgc ctcacttgaa tcgaaggagc agggcagcgg gcaggctagc
      181 cactagccac tgtccactga ccattacatg tccatcgtga ccgatcgcgc cgcaccgccc
      241 gtcagctccc ccacagcgag aatcctcgta gtggacgatg agcgatcgat gcgcgagctg
      301 ctggcgatcg tgctcaagcg cgaggggcat gaggtactcg tggccgaaga cggccggagc
      361 gcgattgcgc tgctcgagaa ggagccgatc gagctcctca tctccgatgt gcgcatgccc
      421 gacgtcagcg gcgtccaggt cctcgcggcc gcgcagcgcg tcaatcccga catcatcggg
      481 atcatggtga ccgcgtttgc ctcgaccgaa acggccgtgg aggcgctgcg cctcggggcg
      541 tacgactatc tgagcaaacc gttcaacgtc gacgagctga aactgaaagt gcggaaggcg
      601 ctcgagcggc ggtcccttca gcaggagaac cagctgctca agcgcgccct tcagagcacg
      661 caccagttct cgaacatcat cggccggagc tccgccatgc aggcggtttt ccagttgatc
      721 cagaccatcg ccccgaccat gagcaccgtg ctcgtcagcg gggaatccgg caccggcaag
      781 gaactggtgg cccgcgccat ccacttcaac tcgccgcgca aggatcgtcc gttcgtggcg
      841 ctcaattgcg gcgcgttgcc cgaaaccctg ctcgagtccg agttgttcgg ccacatgcgc
      901 ggcgccttca cgggcgccga cacgaacaag aaggggctcg tcgaagtggc agagaagggc
      961 acgatcttcc tggacgagat cggggagatg agcccgatga tgcaggtgaa gctgctgcgg
     1021 gtcctccagg agcgcaaatt ccgccggctc ggaggcaccg aggagatcga cgcggacatc
     1081 cgcatcatcg cggccaccaa ccgcgatctc gaaaagctcg ttgccgaggg gagattccgt
     1141 gaagatctgt tctaccggat caacgtcatc ccggtgcggc tgccgcctct gcggaaccgc
     1201 gccgacgaca ttccgttgct cgccgaccat ttcctggtga agtacaaagg tcaggcccgt
     1261 cgcaaggtct cggggatttc gccggacgcg atgcgccttc tccaggccta cgcctggccg
     1321 gggaacatcc gggagctcga aaacgcgatc gagcgcgccg tagcgcttga gaccggcccg
     1381 atggtccagc cctccagtct cccggaacac gtgcagacgg gggtcgtcgg acaaggcgcg
     1441 ggtaacggcc tcgaagcccc gctgccagac tccggctttg atctcgaaca gcacgtcgcc
     1501 gacatcgaac gggagtacat agccgaggcg ctgcgacggg ccggcggagt gaaaagccgg
     1561 gcggccgatc tgctgggcat gagcttccgc tcgtttcgct actacatgaa gaagtacaac
     1621 ctcggataag acaatcctgc ctgcccgttc tcgtattcac actcaaggtg caatctggct
     1681 tgccgttttc cggttgagct tggccccggc cttgcttcaa aggtcacgtc aggagccaag
     1741 cgttgtgagg aattcccgag gtttcacgct ggcggaactg ctcatcgtcg tcgccgtgat
     1801 tggcatcctt gccgccatcg cggtcccggc gctcctgcgt gcacggcaga acgccaacga
     1861 ggcctccgcg gtttcgacca tgcggaccat ttccagcgcg gaggtgacct ttgcggccag
     1921 ctgcgggcgt gggggctacg cccagagcct ggcagacctg agccggccgg cgcccagcgg
     1981 actgacgttt atcggcccgg atctcagcca ggatccttcg atgaagtcag ggtatgagat
     2041 cgtgctgagc gcggaggccg ggtccgcttc tgtgacgccc gccgccggca cgtgcaacgg
     2101 atcggcaaac gatgccgtcc gggcattctg ggccggcgcg cggccgctcc agcttggcgc
     2161 gaccgggcag cgcgcgtttg ccattgatgt gcgcggtgcg atcttcgagg cggccaatgg
     2221 tgcagtccac atcgccaatc ctgttccgcc aggcactacc gtcctgaaat agaaatagaa
     2281 tttgacaatt tctgtcagct taagcttgac a
//
LOCUS       DATIFQ010000149         9713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_434213, whole genome shotgun sequence.
ACCESSION   DATIFQ010000149 DATIFQ010000000
VERSION     DATIFQ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434213"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VLE22_07030"
     CDS             <1..218
                     /locus_tag="VLE22_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14195.1"
                     /translation="AAEQAYRSALDSNRRSLQPSADAALEYVRFLKLRSRDDEAKKLL
                     NEISRWDLGAAEEFLASGEAAPPESNR"
     gene            complement(240..1088)
                     /locus_tag="VLE22_07035"
     CDS             complement(240..1088)
                     /locus_tag="VLE22_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14196.1"
                     /translation="MSWKDLAAMPWDLWRRQVAAIQRLELKKNLWTLRAWWIYLLALG
                     PVILTGAHTVIELYRPHGNTLAEDSMVFAGMFQLFYLRLGIFFGCVGIFTNLFRGEVL
                     ERTLHYYLLSPVRREVLAVAKYVAGLAAAALFFATSVAASFVLISLHLGTEFREHVFR
                     GPGLEHLTWYVLVAVLACAGYGAVFLFLGMVFRNPMLPAALVMIWEGINPFLPSVLKK
                     ASVIFYLKSLCPVSLPPKGVMALIAVETAPTPAWLAIPGLMLVVAAVLAYAAHRARGF
                     EVSYSE"
     gene            complement(1085..2029)
                     /locus_tag="VLE22_07040"
     CDS             complement(1085..2029)
                     /locus_tag="VLE22_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB14197.1"
                     /translation="MNEAAIVFDNVSRFYGDVLGVNGVNLSIPPGVTSLVGPNGSGKT
                     TLMNLMTGLIRPTRGTVSVRGIPPDRPHELFRVVGYCTQFDSFPKSFTGYEFLFSYLL
                     LHGMRATEADRRALEALAQVGLLEAANRKVAAYSKGMRQRIKLAHAIAHDPRVLVLDE
                     PLNGLDPMARAETIALFQQWGEQGRHVIVSSHILHEVDRISDQVILLSHGYVVAEGQI
                     QGVRSEVSDHPIQILVRCLRPAALASRLFEQDHVVEARITDDRRGLAVRTRDADRFYL
                     LLNRLVVESGLQLESVAPVDEDVSSVYQYLIGTQGGAS"
     gene            complement(2026..2925)
                     /locus_tag="VLE22_07045"
     CDS             complement(2026..2925)
                     /locus_tag="VLE22_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14198.1"
                     /translation="MAVYKKTYRPYDGHLTPSWSRFLVVARYAYEDLRRSRFLSFFFF
                     ASFLYPLGCAFTIYLRYNLGVLDAFGLAPPKSFAIEAPFYLTFLGFQSMLAFFLNAFI
                     GPGLVAPDLANNALPLYLSRPFSRAEYVLGKMSVLAILLSLMTWVPGLALFALQGYLA
                     GFQWTMSNLRIPTALFAGSWIWILILSLLSLAMSAWVKWKPVAGALLFGIFFVASGFG
                     AAINGVLKTKWGHLMNVSHLIGSVWVSLFEEPLKRGAGAVFFRVAFGEEIPIWCCWAS
                     LICLCLFCLYLLDRKIRGVEVIR"
     gene            complement(2912..3844)
                     /locus_tag="VLE22_07050"
     CDS             complement(2912..3844)
                     /locus_tag="VLE22_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB14199.1"
                     /translation="MATPVIELENLEVRLGNRLILKRLTGSFSGRAIGLLGPNGAGKS
                     TLINTLLGFLTPSAGTARLFGMDVRRQAKEARGLVGYMPENDSFIAGMTGVRFVRLMA
                     ELAGLPPQQALERAHEAFFWVGLGEVRYRTIGTYSLGMKQMAKLAQAIAHGPKLLFLD
                     EPTNGLDPPARQRMIQLIQEIRDRGDLHLILSSHLLRDVEDCCDEVLILKDGRVAAFC
                     NLEEERRANRRFLELETRGADAAFVGDIEGLGCEAAWSGNGKIKLVLPDGVEVRQLYE
                     LAAVRQVQIRRLNHKRDSLEDIFLKAMEGANGSL"
     gene            3957..5459
                     /locus_tag="VLE22_07055"
     CDS             3957..5459
                     /locus_tag="VLE22_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14200.1"
                     /translation="MASRGTSGPPRGWLWLIPAASWIVPARLRSDWKRKRLEEVRHWW
                     AFLAERGEESPGQQFEVLRHCRRSFSEAVKLRFQVDHVRERMGQFARGPVFAVLVCIL
                     PLAAIVLAGGFSSDIRKIYTPLPYGSGRLAMLSYAGGLGYPLGVPAQDLLVWRERSKS
                     VEGLAYYISRPFNARRPPEPGEERNAQVSPDFFQVLGVRALLGRTLQPDDAPGATTNA
                     VLSHFYWQERFGGDTGILGKTVTLGERPMRIVGVLPADFWFRSRNIHYWTPLRDIEPK
                     DRRLRLYGAVARLQPEVTMELAQEELRRLASEQSGGPHGAGVRLLPVEHPGRSAMRTY
                     IVGLALTLLLAAVIALAQISRAWWLSTEWKRRTTAIRYWSFFSLKTVFPAAAWSALCA
                     QIVAANAVSLNPVLGLSLIISILYLLGLAGIVRFSYVDQRLRCPVCLRSLAMPVTMGS
                     WSSWLLEPASTEFLCEQGHGSLYVSETHSSGTESERWENLDDSWRDLFKK"
     gene            complement(5498..7984)
                     /locus_tag="VLE22_07060"
     CDS             complement(5498..7984)
                     /locus_tag="VLE22_07060"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HSB14201.1"
                     /translation="MISDENKSDSILDNVIAGIRDEQIDPLAADRAGARVLARLSSHS
                     PLRGCADFQALIPEYRAGALPEARALLIKDHLHECVACRKAASGETARITLSAPRRWA
                     LPVWRWAIAAMLAAAVGLGTWEVWNRLGSSGGRATVQAADGELYRVAASGLERVGPGV
                     ELPAGAEIRTAGGAAVVGLADGSLVELRERTGLSVSGTRRDTTVRLTRGSVIVQAAKR
                     RFGHLYVDTRDCKVAVTGTVFSVSSGIKGSRVAVIEGEVKVSQQGKVAVLRPGEQFAS
                     SDSLSPVPVEEEIAWSRDREKHMALLREFAVLRNKLLEVRLPGVRYSSRLIGLLPAET
                     AVYTAVPNLGQALTEAQQIFEQRVRESAVLREWWEQKTDGGKRAAELEQSISKIREVS
                     EYLGEEVVIAAAVDANGKLQDPLILAEVKRAGLREFLEKEFPGIEKSGHLLLRPDLLV
                     LSPSAAALGAVSALVDAGQSGGFASTPFGTRVTAAYHDGAGLLFSADLERIVPGRDKA
                     PAGLNEMKYLVVEQREVAGKSGTHAELGFVGPRHGIASWIAAPGPLGALDFVSPDASF
                     AAAFLIKNPAAVIDDVLTLNPEVRKGLADADAMLGLSLQKDLAGTMGGEFAFALDGPM
                     LPVPSWKLAIEVYDPQRLQSALEKLMEAANREAARQGRNPVQIRREVAGGRTWYTVIV
                     PGAKPFPELHYTFLDGYVLAGASQELLERAIQNRGVGYTLARSSGFAALLPADGHSDF
                     SGLLYHNLGPALAPVVQGLGVAGALAPESQKSAEAFSADLKPAVITFYGEEDRITVAS
                     QGSLLGLGLGNLVGVPGPFHGFTRPHVRSR"
     gene            complement(7981..8535)
                     /locus_tag="VLE22_07065"
     CDS             complement(7981..8535)
                     /locus_tag="VLE22_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB14202.1"
                     /translation="MATATASPADPANTAVADIEQVFREHHAYVFRVACRVTGNATDA
                     EDVMQTVFLGLLRRDQSDPIGSLDSYLYRAAVNSALDVVRARRGGSNVPLEEVAAWLA
                     EDPSRAPDRARSSTELRHWLRAAIGRLSPRAAGMFVLRFFEGMDNIEVARIFETTPGT
                     VAVTISRTRDQLERELRSFQGGKS"
     gene            complement(8646..>9713)
                     /locus_tag="VLE22_07070"
     CDS             complement(8646..>9713)
                     /locus_tag="VLE22_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HSB14203.1"
                     /translation="TDTGEWLANCSACCVPEISNALARSSIPFHVVSGVLFEDDAAWR
                     EIAGWCRAAAVTRAIRESRIGFLGHTYPGMLDMYSDFTMVHAKLGAHVEVLEMCDLSL
                     HAAHVGEDEVEEKLRAARETFQVDGSVAPDDLRWAARVAAGLDHFVEDFDLDGLTYYY
                     RGLDGNEYERLGAGMILGNSLLTARGIPASGEGDIKTCIAMMMLDRLGAGGSYTEFYA
                     MDFRENFLLMGHDGPGHLAISDEKPVLRGLGLYHGKRGYGVSVEFKVKAGPVTILGLT
                     QTAQGGFKLIGAEGESLPGPILRIGNTNSRLRFGLPPAEFINRWCAEGPTHHCALGVG
                     HYLNAIAKLSALLEIPLVTVG"
BASE COUNT         1926 a         3009 c         3056 g         1722 t
ORIGIN      
        1 ctgccgctga gcaggcgtat cgttccgcac ttgactcgaa ccgacggtct ctgcaaccta
       61 gtgcggacgc cgccttggag tatgtccggt tcctgaagct ccggtcacga gacgatgagg
      121 caaagaagct gctcaatgag atctcacgct gggacctcgg cgcagcggag gagtttcttg
      181 catccggtga ggctgcgcca cccgagtcga accgttagtg ggactacatc aaatcactgt
      241 cactcgctgt agctgacctc gaagccgcgg gcgcggtggg cggcgtaggc gagcacggca
      301 gccaccacca gcatcaggcc cggaatggcc agccaggcag gcgtcggcgc cgtttccacg
      361 gcgatcagcg ccatgacgcc tttcggcggg aggctcacag ggcagagcga cttcagatag
      421 aaaatgacgc tcgccttctt tagtacggac ggaaggaatg gattgatccc ttcccaaatc
      481 atcaccagcg ccgccggaag catcggattt cgaaacacca tacccagaaa caggaagact
      541 gctccatatc cagcgcaggc gagcaccgca acaaggacgt accaggtgag atgttcgagc
      601 cccggcccgc gaaacacgtg ctcccggaat tccgtcccga ggtgcagcga tatcagaacg
      661 aacgaggccg ccacgctggt tgcgaaaaac aaggccgccg ccgcaagccc cgcgacatac
      721 ttcgcaaccg caaggacttc ccggcgtacc ggcgagagta ggtagtagtg tagcgtccgc
      781 tcgagcactt ctccccggaa caggttggtg aagatgccca cgcacccgaa gaagatcccg
      841 agcctcaagt aaaagagctg gaacattccg gcaaagacca tcgagtcctc agcaagggta
      901 ttgccgtgtg gacgatacaa ctcaatcacc gtgtgcgcgc cggtgagaat gaccggtccg
      961 agggcaagca ggtagatcca ccatgcgcga agcgtccaga ggttcttttt cagctcgagc
     1021 cgctgaatcg ccgcgacctg tctgcgccac aggtcccacg gcattgcggc aaggtctttc
     1081 cagctcatga cgcccctcct tgtgtgccga tcaggtactg gtagacggag ctcacgtcct
     1141 cgtccactgg tgcgaccgac tcgagctgga gcccgctttc caccaccaac cggttcagca
     1201 agaggtagaa gcggtcggcg tctcgggttc ggacggcgag acctcgccgg tcatcggtga
     1261 tgcgggcttc gacgacgtga tcctgctcga acagtcggga ggcgagcgcc gccggccgga
     1321 gacagcgaac gaggatctgg atcgggtgat cgctgacttc gctgcgaaca ccctgaatct
     1381 gcccctcggc cacgacgtag ccgtggctca acaggatcac ctgatcggag atacggtcca
     1441 cctcatgaag aatgtggctg gaaacgatca cgtgtctgcc ttgttctccc cactgctgga
     1501 acagtgcgat ggtttcggcg cgcgccatcg gatcaagacc gttaagcggc tcgtcgagaa
     1561 ccaagacgcg gggatcgtgc gcgatggcat gggccagctt gatccgctgg cgcataccct
     1621 tgctataggc ggctaccttc cggtttgccg cttcgagcag cccgacctgg gctagcgcct
     1681 caagagctcg tcggtccgcc tcggtggctc tcatcccgtg caggaggaga taggagaaca
     1741 gaaactcgta accggtgaag ctcttaggaa aagaatcaaa ctgagtacag taaccgacca
     1801 cccggaagag ctcgtggggc cggtccggcg gaataccgcg gacactcacc gtgcctcttg
     1861 tcgggcggat aagcccggtc atgaggttca tcagcgtcgt cttgcccgag ccgttcgggc
     1921 cgacgaggct tgttacccca ggagggatgg agagattgac gccgttgacg ccaagcacgt
     1981 cgccgtaaaa gcgggagacg ttgtcgaaga caatcgcggc ttcgttcacc ggatcacctc
     2041 cacgccgcga attttccggt cgagcaaata cagacagaac aggcagaggc agatcaggga
     2101 cgcccagcag caccagatcg gaatttcctc gccgaaggcc acccggaaaa agaccgcgcc
     2161 agcgccgcgc ttcaatggct cttcgaacag cgatacccat acggaaccga tcaggtggct
     2221 cacgttcatg aggtgtcccc acttcgtctt gaggaccccg ttgatcgctg cgccgaaccc
     2281 ggacgccaca aagaagatac cgaacaggag cgcaccggcc acaggcttcc acttcaccca
     2341 cgctgacatc gccagagaca ggagggaaag gatcagaatc cagatccatg agccggcgaa
     2401 caaagccgtc gggatgcgaa ggttcgacat cgtccactgg aaacctgcca ggtagccttg
     2461 cagggcgaag agggccagcc ccggaaccca cgtcatcaac gacagcagaa tggccagcac
     2521 ggacatcttg ccgagcacgt actcagcccg cgagaaaggc cgtgagagat acagaggcag
     2581 ggcattattg gcgagatccg gagccacgag tccaggcccg atgaatgcgt tcaggaagaa
     2641 cgccagcatg ctctggaagc cgagaaacgt caggtagaac ggggcttcga tggcgaaact
     2701 cttgggaggg gccaagccaa atgcatcgag aacgcccagg ttatatcgta ggtatatcgt
     2761 gaaagcgcag cccaaaggat agaggaagct ggcgaagaaa aagaagctca ggaagcgcga
     2821 gcggcgtaga tcctcatacg cgtagcgggc caccactaga aaacgagacc aggatggcgt
     2881 caagtgtccg tcgtacgggc ggtacgtctt cttatagact gccattggcg ccctccatcg
     2941 ctttcaggaa gatgtcttcg agcgaatcgc gcttgtggtt gaggcggcga atctgcactt
     3001 ggcgcaccgc ggcgagttcg tagagctggc gaacctccac tccgtccgga agcacgagct
     3061 tgatctttcc gttgcccgac caggcggcct cgcagcccaa tccctcgatg tcgccgacga
     3121 aggccgcgtc tgcgccgcgg gtctcgagtt caaggaatcg gcggttggca cgccgctctt
     3181 cttccaggtt gcagaaggcg gcaactcttc cgtccttaag gataagcact tcgtcacagc
     3241 agtcttcaac gtctcggagc agatgcgacg agagaatcag atggaggtca ccccgatcgc
     3301 ggatctcctg gatgagctgg atcattcgct gccgcgcggg cgggtcgagg ccgttggttg
     3361 gttcgtcgag gaacagcagc ttggggccgt gcgcgatggc ctgagcgagt ttggccatct
     3421 gtttcatccc tagcgaataa gtgccgatcg tgcggtagcg gacttcaccc agtccgaccc
     3481 agaagaatgc ctcgtgggct cgctccaggg cctgttgcgg ggggagtccg gccagttcag
     3541 ccatgaggcg gacgaagcgg accccggtca tgccggcgat gaacgagtca ttctccggca
     3601 tgtacccgac gagcccccgg gcctctttgg cctgccggcg gacgtccatg ccgaagaggc
     3661 gggccgttcc tgctgaaggg gtgaggaagc cgagaagagt attgatgagc gtcgactttc
     3721 cggcaccgtt cgggccaaga agaccgatgg cacgtcctga gaaggacccg gttaggcgct
     3781 tcagaatcag ccggtttccg agcctgacct ccaggttttc caactcaatg accggcgtcg
     3841 ccatgtctct ggatacgcac tcggaggcga cggtgttccc cctgatgcga atgattccac
     3901 ctacgcggaa acagcgcgga cccgtacggc atctaaccta gtggagaagt gttgctatgg
     3961 cgagcagggg aacctcagga ccgccccgcg gctggctctg gcttatcccg gcggcaagtt
     4021 ggatcgttcc cgcgaggttg cggtccgact ggaagcgaaa gcggctggag gaggtgaggc
     4081 actggtgggc cttcctcgcc gagcgtggcg aggagagccc cgggcagcag ttcgaggtgt
     4141 tgcgccactg ccggcgttcg ttttcggaag cggtcaagct tcggttccag gtggatcatg
     4201 tccgggagcg catggggcag ttcgcacgcg gcccggtgtt cgccgtcctc gtgtgtatcc
     4261 tcccgctcgc ggcgattgtg ctggccggcg gcttctcttc ggacatccgg aagatctaca
     4321 ctccgttgcc ttacgggagt ggtcggctag cgatgctctc gtacgccggc gggctgggat
     4381 acccgcttgg agtcccggca caagacttgc ttgtgtggcg cgagagaagc aaatcggttg
     4441 aggggctggc gtattacatc tcgcgcccgt tcaatgctcg aaggccgcct gagccagggg
     4501 aagagcgcaa cgcgcaagtg tctccggatt tcttccaggt actcggcgtt cgagcactcc
     4561 ttggcaggac actccagccc gacgacgcgc caggagccac caccaacgcg gtactgagcc
     4621 acttctactg gcaagagcga ttcggaggcg acaccgggat tctcggcaag acggtaacgc
     4681 tcggcgagcg gccgatgaga atcgtcgggg tccttccggc cgatttctgg ttccgctccc
     4741 gcaatatcca ttactggacg ccgctccggg acatcgaacc gaaagaccgg cgcctccgcc
     4801 tgtatggcgc ggtcgcgcgc ctccagccgg aggtgaccat ggaactggca caggaggaat
     4861 tgcgccggct tgcaagcgag caaagcgggg gcccgcatgg tgcgggcgtt cggctgctgc
     4921 ctgtggagca ccctggccga tctgcaatgc gcacgtatat agtggggcta gcgctcacac
     4981 tgctcttggc ggcggtgatc gcactggcac agatctcacg cgcctggtgg ctctccaccg
     5041 aatggaagcg gcgtaccaca gcaatccggt actggtcgtt cttctcgctc aagaccgttt
     5101 tcccggcggc ggcatggtcg gcactgtgcg cacagatcgt cgcggcgaac gcggtcagct
     5161 tgaacccggt actcgggctg agcttgatca tcagtattct ctatctgctc gggctggccg
     5221 gaatcgtgcg tttctcgtac gttgatcagc ggctgcgttg cccggtgtgt cttcgaagtc
     5281 tggcgatgcc ggtgacgatg ggttcctgga gtagttggct gctggagccg gcgagcacag
     5341 agtttctctg tgagcaggga cacggttcgc tctatgtttc cgagactcat tcgagcggca
     5401 cggaatcgga gaggtgggag aacctcgatg acagttggag ggacctgttc aagaagtgag
     5461 tatggggcgc acgctgggtg cgcccctaag caaagaccta ccgggaccgt acatgagggc
     5521 gggtgaaccc gtggaaaggt cctggaacac ccacgagatt gccaagcccc aggccgagca
     5581 aactgccctg gctggcaacc gtgatacggt cctcttctcc atagaaggta atcacggccg
     5641 gcttcaggtc tgcggagaac gcttccgccg acttctgact ctccggcgca agagcgccgg
     5701 ccactccgag tccctggacg actggggcaa gggccggccc aaggttgtga tagagcaacc
     5761 cggagaagtc cgagtgaccg tccgccggta gcagtgccgc gaaaccggag gaacgagcca
     5821 gcgtgtaacc cacgccgcgg ttctggatgg cccgctccag cagttcctgg ctggcgcctg
     5881 ccaacacgta gccgtcaagg aatgtgtagt gcagctcagg gaagggtttt gcacccggga
     5941 cgatcacggt gtaccaggtt ctgcctccgg caacttcccg gcggatctgc acagggttcc
     6001 gtccctgccg cgccgcttcc ctgttcgccg cctccatcag cttctcgagt gccgactgga
     6061 gccgctgtgg gtcgtagacc tcgattgcca gcttccacga tggcaccggc agcatcgggc
     6121 cgtctaacgc gaaggcgaac tcaccaccca tcgtcccggc aaggtccttc tggagactca
     6181 acccgagcat ggcatcggca tcggcgaggc ccttacggac ttctgggttc aaggtgagca
     6241 catcgtcaat gacggcggct gggttcttga tcagaaacgc agcggcgaag ctcgcgtccg
     6301 gggagacgaa atcgagcgca ccgagcggtc ccggagcagc aatccaggaa gcgatgccat
     6361 ggcgcggacc aacgaagccg agttcggcgt gcgtgccgct cttgccggca acctcacgtt
     6421 gctcgaccac aagatacttc atctcgttga gccctgcagg tgccttgtcc cgtcccggca
     6481 cgatacgctc gaggtcggcg ctgaacaaca gacccgcgcc gtcgtggtaa gcggcagtca
     6541 cgcgggtgcc gaacggagtg gaggcaaagc cgcctgattg accggcatcc accagggcag
     6601 ataccgcgcc gagagccgcc gcgctcggcg aaagaaccaa caggtctggt cgcagcaaca
     6661 ggtgaccgct cttctcgata ccgggaaatt ctttctcgag aaactcgcgc aggccagccc
     6721 tcttcacctc ggcgaggatg agagggtcct gaagttttcc gttggcatcg accgccgccg
     6781 cgatgacaac ttcctcgccc aagtactcac tgacctcgcg gatcttcgaa atggattgtt
     6841 ccaactcagc ggcccgcttg ccgccgtccg tcttctgttc ccaccactca cgcaggacgg
     6901 cgctttcacg gacgcgctgt tcgaagatct gctgcgcctc ggttagagcc tgtccgaggt
     6961 tgggaacagc ggtgtacacg gcagtctccg ccggcagcag gccgatcagc cggctggaat
     7021 agcggacgcc cggtaaccgc acttccagaa gcttgttgcg gaggacggcg aactcccgga
     7081 ggagcgccat gtgcttttca cggtctcggc tccacgcgat ctcctcttcg accggaaccg
     7141 gcgagaggct gtcgctgctg gcgaactgct cgccgggccg cagcacggca acctttccct
     7201 gctgcgagac cttcacctcc ccttcaatca ctgccactcg cgaccccttg atgccactgc
     7261 tgacgctgaa cacggtgccg gtcacggcga ccttgcagtc ccgggtgtcg acatacaggt
     7321 gtccgaaccg ccgcttcgcg gcctggacga tcacgctgcc acgggtcaga cggacggtgg
     7381 tgtctctgcg cgtgccgctg acggacaggc cggtgcgctc tcgcagttcg acgagcgaac
     7441 cgtcagccaa ccccacgacg gcggctccgc cggcggttcg gatttccgcg cctgcaggca
     7501 gctcaacgcc aggtccgacg cgttccagac cgctggcagc gactcgatag agctcaccgt
     7561 cggcggcctg aaccgtcgcg cggccgccgg acgagcctaa acggttccac acctcccacg
     7621 ttcccagccc gacggcagct gccaacatgg ccgcgatcgc ccatcgccag acggggagcg
     7681 cccagcggcg cggggcggac aaggtgatcc gagccgtttc tccggatgcc gccttccggc
     7741 aggcgacgca ctcatgaaga tgatccttga tcagaagcgc acgcgcttca ggcaaagcgc
     7801 ccgcgcggta ttcggggatc aaagcctgga agtcggcaca gccccgcaac ggcgagtgcg
     7861 aggacagacg tgccagcacg cgggctccgg cgcgatccgc tgcaagcggg tcgatttgtt
     7921 cgtcgcggat gcctgcgatg acgttgtcga gaatggaatc ggacttgttt tcgtcggaaa
     7981 tcatgactta cctccctgaa atgagcgaag ctcccgctcg agctgatcgc gcgtccggct
     8041 gatggtcacg gccactgtgc ctggcgtggt ctcgaagatg cgagcgacct cgatgttgtc
     8101 catcccttcg aagaaccgga ggacaaacat cccggcggcg cgcgggctca ggcggccgat
     8161 ggcggcgcgt agccagtgac gcagttcagt ggacgagcgt gcgcgatcgg gagctcgaga
     8221 gggatcttct gcgagccacg ccgcaacctc ttccagcggt acattcgagc cgccacggcg
     8281 cgcccgtaca acgtcgaggg cggagttcac ggcggcgcga taaagatagc tgtcgaggct
     8341 gccgatgggg tcgctttggt cccggcggag taggcctagg aagacggttt gcatgacatc
     8401 ctcggcgtcg gtggcattac cagtcacccg gcaggcgaca cgaaagacgt aggcgtgatg
     8461 ctcccggaac acctgttcga tgtccgccac tgcggtgttt gccgggtcgg ctgggcttgc
     8521 ggtcgctgtt gccaccggat tcctcccgtt tcgctcttca ttcatgagac ggctccgtag
     8581 gccgattctt aacagcctgt tctgggaaac ggagaccggg aggaaaaagt tccgagaagg
     8641 tccgatcagc ccaccgttac gagtgggatc tcgagcagcg cactgagctt cgctatggca
     8701 ttgagatagt ggcccacgcc cagtgcacag tggtgggtcg ggccctccgc gcaccaacgg
     8761 ttgatgaatt ccgcgggcgg cagtccgaaa cgcaagcgtg agttggtgtt accgatgcgt
     8821 aggattggac cggggagcga ctcgccctcg gcgccgatta gcttgaaacc tccttgcgcc
     8881 gtctgggtga ggccgagtat ggtaaccggc cctgccttaa ccttgaactc caccgacacc
     8941 ccgtaaccgc gcttgccgtg gtaaagcccc aaaccgcgga ggaccggttt ttcgtcactg
     9001 atcgcgaggt gcccggggcc gtcgtgcccc atcagcagga agttctcgcg gaaatccata
     9061 gcatagaact cagtatacga gccgcccgcg ccaaggcggt ccagcatcat catagcgata
     9121 caagtcttga tgtccccctc gccgcttgcc ggaatgccgc gcgcggtcag cagcgaatta
     9181 ccgaggatca tgcccgctcc aaggcgctcg tactcattgc cgtcaagccc gcggtagtag
     9241 tacgtgagac cgtcgaggtc gaaatcctcg acgaagtggt caagtccggc ggcgacgcgc
     9301 gcagcccacc ggaggtcgtc cggggcgaca ctcccgtcta cctgaaacgt ttcgcgagcg
     9361 gcccgcaact tctcctcaac ttcgtcctcg ccaacgtgcg ccgcgtgaag cgagagatcg
     9421 cacatctcca gcacttcgac atgggctccg agcttcgcat gaaccatggt gaagtccgag
     9481 tacatgtcga gcattcccgg gtacgtgtgg ccgagaaaac cgatgcggct ttcgcgtatc
     9541 gctcgagtga cggcggcggc acggcaccag ccggcgatct cccgccacgc cgcatcgtct
     9601 tcgaacagca cgccggaaac gacatggaac ggaattgagg aacgtgcaag cgcgttcgag
     9661 atctccggaa cgcagcaggc cgagcagttc gcaagccact cgccggtgtc ggt
//
LOCUS       DATIFQ010000150         4276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1200345, whole genome shotgun sequence.
ACCESSION   DATIFQ010000150 DATIFQ010000000
VERSION     DATIFQ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200345"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VLE22_07075"
     CDS             <1..765
                     /locus_tag="VLE22_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB14204.1"
                     /translation="GAAVLGGSLCGRKGVDRMAVPHEEFHREFDEARSEEKAALQPTA
                     LGADSETGPTPVRWGELVEQIQLGDPRGMEELYRLFSRGIRFYLCRQLGPQELDDKVH
                     DTFVIVVQAIQRGDLREPDRLMGFVRTVVRRQIAAHIDSAVHNRRDFEDLETGAAVYD
                     HLATPEERVISRQEMEIMEAVLRSVSVRDREILTRFYLMEQPQQQICEAMNLTETQFR
                     LLKSRAKARFGELGRRRLKHRIIPKVSLRKLAGTGH"
     gene            829..1464
                     /locus_tag="VLE22_07080"
     CDS             829..1464
                     /locus_tag="VLE22_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14205.1"
                     /translation="MQAEFGEHAGEEEVELYSMGRLEGTRLEAIEEHLLVCASCQDRL
                     EDMDIYVRAARAATAKLAAEPSQKTPGFLGRFRAFALSYEPVLITVAVAVVVIGVYWI
                     ARPSQEKLNSPVAVAVLETVRGESTAATVSRDSRPLLRIDVSELAPSSSYRVELVNAQ
                     GDTIWRTDATPSSGKLSVPVETVLAGGRYWVRLYSPAPERTLLREYELKAE"
     gene            complement(1477..2136)
                     /locus_tag="VLE22_07085"
     CDS             complement(1477..2136)
                     /locus_tag="VLE22_07085"
                     /inference="COORDINATES: protein motif:HMM:NF036876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HSB14206.1"
                     /translation="MSGEKIGLMVRADAGPGILHQLTGVIARHSGDITLVEITESVPP
                     DSRIYFEIDLPGDSSALAAEMRSLPVVREVAIVKTFQKIYGKRIIIMGGGAQVGQVAI
                     GAISEADRHNIRGEHISVDTIPLVGEQSLADAVRAVARLPRARALVLAGSLMGGEIEQ
                     AVREVREQGLLVISLNMAGSVPDAADLVVSDPVQAGVMAVMAVADTAKFNVARLKQRV
                     F"
     gene            complement(2137..2667)
                     /locus_tag="VLE22_07090"
     CDS             complement(2137..2667)
                     /locus_tag="VLE22_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HSB14207.1"
                     /translation="MERAARVRLLLMDVDGVLTDSRIYFLPAPDGSWTETKGFDSQDG
                     IALHWLHWKGIKTGIISGRKSPAVELRAQQGNMAYCYQGNIEKIPIIEEILAKEGVTR
                     DQVAYIGDDLTDIVVMRRVGFGVATANARPEVKECAHYVTQIEGGRGALREVAELLLK
                     AQGYWEEILRKYEVNV"
     gene            complement(2728..3810)
                     /locus_tag="VLE22_07095"
     CDS             complement(2728..3810)
                     /locus_tag="VLE22_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HSB14208.1"
                     /translation="MSYGLNIRPEGALDFVSLGALVHRLDPGIIPFRKATKCQIHVSG
                     GEFNCAANLANCFGLQTGITTAMVDYPIGDLIAERVRTMGVKPFYKRFTHNGVNGPNM
                     ATVYSDRGFGVRGPVVFYNRSNEAGALLKPGDFNWKEIFGGGVRWFHSGGIFAALSET
                     TGELIVEAMKAAKAAGAVTSFDLNYRAKLWNIWGGQERAVAVVKRIVENVDVLVGNEE
                     DLQMGLGIPGPEVTKKSKLDPSVFFGMIDQVLAKHPQVKIVVTTLREVLSTNHHSWSA
                     VAWINGKTYVAPTAELNVYDRVGGGDGFASGFIYGILSGEPEEEAVKLGWAHGALLTT
                     FPGDTTMATADQVRAFAKGGSARIQR"
     gene            complement(3847..>4276)
                     /locus_tag="VLE22_07100"
     CDS             complement(3847..>4276)
                     /locus_tag="VLE22_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14209.1"
                     /translation="REAAKEYKWNLNYGGIALVWRGGCIIRSAFLGKIKEAFDRDKNL
                     SNLLMDKYFRGVIARRQTSWRRAVAKAVQMGVPVPAFTTALAFYDGYRSERLPANLLQ
                     AQRDYFGAHTYERVDKPRGQFFHTNWTGRGGTVASGQYTV"
BASE COUNT          806 a         1280 c         1336 g          854 t
ORIGIN      
        1 ggagccgcgg tactgggcgg ctcactttgt ggccggaaag gagtagaccg gatggcagta
       61 ccgcacgagg aattccatcg tgaattcgac gaggcgcgct cggaggaaaa ggccgcattg
      121 cagccgactg ccttgggcgc cgatagtgag accgggccga ccccggtgcg ttggggcgaa
      181 cttgtagaac agattcagtt gggtgatccc cgcgggatgg aagaactcta ccggcttttc
      241 tcacgtggga tccgcttcta cttgtgccgc cagctaggtc cccaggaact cgacgacaag
      301 gtgcatgaca cgttcgtgat tgtcgtacaa gcgatccaac gcggcgacct tcgcgagccg
      361 gaccggctta tggggtttgt tcggaccgtt gtccgccggc agattgcagc acacatcgac
      421 agcgccgtac acaaccggcg ggacttcgag gacctcgaaa cgggtgctgc ggtttacgat
      481 cacctcgcga cccctgagga acgggtgatc tcgcgacagg agatggagat tatggaggca
      541 gtcctacgca gcgtttcggt tcgcgaccgc gagattctga cgcgcttcta tctgatggaa
      601 caaccccagc aacagatctg cgaggcgatg aatctgacgg agacccagtt ccgcctgctc
      661 aagtcccggg cgaaggcgcg tttcggagaa ctgggaagac gccggctcaa acaccggatc
      721 atcccgaaag tttccttgag aaaattagcg ggaacggggc actaagtgtc aaaaaggctg
      781 gtgtcgtggc ggcggactgc atagaccgct gtcagaaagg gtgctgtcat gcaggccgaa
      841 ttcggcgaac acgccggcga agaggaggtg gagctgtact ccatggggag gctcgaaggg
      901 acgcgactgg aagcaatcga agaacatctg cttgtctgcg cgtcctgcca agaccggctt
      961 gaggacatgg acatatacgt acgcgcggcg cgtgccgcga cggcgaaact ggccgcggag
     1021 ccgtcacaga agactccggg ttttcttggc cggttccggg cgtttgcgtt gagttatgag
     1081 ccggtgttga ttacggttgc cgttgcggtt gtggtgatag gtgtctattg gattgcgcgg
     1141 ccgagccagg agaagctcaa ttccccggtg gccgtggctg tcctcgaaac cgtccggggt
     1201 gaaagcacgg cggcgacggt gtcccgcgac agccggccgc tcctgcgcat cgacgtttca
     1261 gaattggcgc cgtcttcctc ctaccgtgtg gaacttgtga acgcgcaggg ggatacgatc
     1321 tggcgaacgg acgccacccc atcgagcggc aaactcagtg tgcctgtgga gacggtcctt
     1381 gcgggaggca ggtactgggt tcggctatac agcccagccc cggaacgaac actgctcaga
     1441 gaatacgagt tgaaggcgga gtagacgttt cctcgctcag aagacgcgtt gtttcagacg
     1501 cgcgacgttg aacttggcgg tatcggcgac ggccattacc gccatcacgc cggcctggac
     1561 gggatcgctc actacgaggt ccgcggcgtc gggaacgctc ccggccatgt tgagactgat
     1621 cacgagcagg ccctgctccc tcacctcccg aactgcctgt tcgatctctc cccccattag
     1681 cgagccagct agcacgagcg cacgagcgcg cgggaggcgc gcgacggcgc gaaccgcatc
     1741 tgccagcgac tgctcgccga ccagcgggat ggtgtccacg gagatgtgtt cgccgcggat
     1801 gttgtggcgg tcggcttccg agatcgctcc gatggccact tggccgacct gggcgccgcc
     1861 gcccatgatg atgatcctct ttccgtagat cttctggaaa gtcttgacga tggcgacctc
     1921 ccggacgacg ggcaggctcc gcatttccgc cgcaagcgcc gagctgtccc caggcaggtc
     1981 gatctcgaag tagattcgcg aatcgggcgg cacgctttcg gtgatctcaa caagcgtgat
     2041 atcgccgctg tgccgggcaa tcacgccggt gagctgatga agaatgcccg gaccggcgtc
     2101 agcgcgaacc atcagcccga ttttttcgcc ggacatctag acattcacct cgtacttgcg
     2161 gagaatctcc tcccaatacc cttgcgcttt caaaaggagt tccgcgacct cacgcagagc
     2221 gccgcgccca ccctctatct gggtgacgta gtgggcgcac tccttcacct caggacgggc
     2281 gttagcggtc gcgacaccga acccgacgcg ccgcatcacg acgatgtcag tcaagtcgtc
     2341 gccgatgtag gcgacctgat ctctggtgac gccttctttc gcgagaatct cttcgatgat
     2401 cgggatcttc tctatgttgc cttggtagca gtaggccatg ttgccttgct gcgcgcgcag
     2461 ttcgacggca ggtgacttcc ggccgctgat gataccggtc ttgatgccct tccagtgaag
     2521 ccagtgaaga gcgatcccat cctgcgaatc gaagcccttg gtttctgtcc aggagccgtc
     2581 cggcgccggc aggaagtaga ttctgctgtc agtcagtacg ccgtcgacgt ccatcagaag
     2641 gagtcgcacg cgtgcggcgc gttccatgaa atccaccgta gttctcctgt gcagaaagag
     2701 ggccgggcgc acgcccggcc catcatttta ccgctggatg cgggcggagc cgcccttagc
     2761 gaaagcgcgg acctgatcgg ccgtcgccat agtcgtgtcc cccggaaatg tcgtgagaag
     2821 cgctccgtgc gcccacccga gcttaacggc ttcctcttca ggttcgccgc tgaggatgcc
     2881 atagatgaat ccggaagcaa agccgtctcc gccaccgaca cggtcgtaga cgttgagttc
     2941 ggcggtcggg gcgacgtagg tctttccgtt aatccacgcc accgcgctcc aactgtggtg
     3001 gttggttgag agaacttcac ggagtgtcgt cactacaatt ttgacctggg ggtgcttggc
     3061 caggacctgg tcgatcatcc cgaagaacac actggggtcg agcttggact tcttggtgac
     3121 ctcaggtccg ggaatgccga gacccatctg gaggtcttcc tcgttgccga ccaggacatc
     3181 gacattctcc acgatgcgct ttacgacggc gactgcccgc tcctggccgc cccagatgtt
     3241 ccacagcttg gcgcgatagt tcagatcaaa cgatgtgacc gctccggcgg ccttggctgc
     3301 cttcatcgcc tcgacgatca gttcgccagt tgtctccgag agcgccgcga agatgccgcc
     3361 gctgtgaaac catcgcactc ctccgccgaa gatttctttc caattgaaat cgccgggctt
     3421 cagaagggcg ccggcctcgt tgctgcggtt gtagaaaacc accggtccgc gaactccgaa
     3481 gccacggtcg ctatagacgg tagccatgtt cgggccgttg acgccgttat gtgtgaatcg
     3541 cttgtagaag ggctttacac ccattgtccg cacccgctcg gcgatcaggt cgccgatcgg
     3601 atagtccacc attgctgtag tgatgccggt ctgcagaccg aagcagttcg ccagattggc
     3661 ggcgcagttg aactcgccgc cactcacgtg gatctggcat ttggtggcct tgcggaaggg
     3721 gatgatgccg gggtccaggc ggtggaccag tgcgccgaga gaaacgaaat ccagagcgcc
     3781 ctccgggcgg atattcaaac cgtagctcat tctattctcc tttgtgctgg gtctcgccgg
     3841 cgccggctat acggtgtact gtccagacgc gaccgtgccg ccgcgtccgg tccagttcgt
     3901 gtggaagaac tggccgcgtg gtttgtcgac ccgctcgtag gtgtgcgctc cgaagtagtc
     3961 gcgctgagcc tggagcaggt tcgcgggcag gcgctcgctg cggtagccgt catagaacgc
     4021 aagcgcggtc gtaaatgccg gtaccggcac gcccatctgc accgctttgg caacagcacg
     4081 gcgccaggag gtctgacggc gggcgatcac gccacggaag tacttgtcca tcaataagtt
     4141 gctcaggttt ttgtcgcggt cgaacgcttc tttgatcttg ccaaggaagg ccgaacggat
     4201 gatgcagcct ccgcgccaca cgagcgcaat gccgccgtag ttgaggttcc acttatactc
     4261 tttcgccgcc tcgcgc
//
LOCUS       DATIFQ010000151        10261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1967594, whole genome shotgun sequence.
ACCESSION   DATIFQ010000151 DATIFQ010000000
VERSION     DATIFQ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10261
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967594"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..522)
                     /locus_tag="VLE22_07105"
     CDS             complement(28..522)
                     /locus_tag="VLE22_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB14210.1"
                     /translation="MRYFITFACYGARLRGEESGSVDRRHNLVGSRLLEPDAQRVIAE
                     RREMLQYPYVLNEAGRTVVLAAIQRHCAYRGWNLLAAHVRSNHVHTIVEAETRPERIM
                     NEFKSYASRELNRLTSDGPDRKRWARHGSTRWLWKDEDVRHALQYVIDEQGEPMALFD
                     AEGL"
     gene            685..2304
                     /locus_tag="VLE22_07110"
     CDS             685..2304
                     /locus_tag="VLE22_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HSB14211.1"
                     /translation="MWEQHYTPVGGSLAWSTLVAALPIFALLYLLGVRRKPAWKAALF
                     GLVAAAVVALVAYRMPVDKMLSAVGFGAAFGLFPIGWVVFCAILLYRVTLETGKFEIV
                     KDSVGSLTDDRRLQALLIAFAFGAFIEGAAGFGTPVAVAAAMLTGLGFSPFYAAGICL
                     LANTAPVAFGSIGTPILTLNAVTGLPLEGLSAAVGRICAPVSLIVPAYLIAVMGGWKA
                     MKGVLPAVIACGVSFAGTQILVSNYLGPQLTDILSSLAALGSLVLLFLVWKPKDRYAL
                     EGDHVMDVHRKHHSARELCLAWGPYGILVVFVLLWGLYKAPLNKVTLLFNWPGLHEQV
                     IQMAPVVANPSPYKAVYRLEWLAAPGTACLFAAILSAMLLGMSPRQFGRVFAGTVKQL
                     LLSELTIASVLALAFLMNYSGATATLGLAFAATGVLFPFFSSMLGWLGVFLTGSDTSA
                     NALFGNLQVVTAGRLDLSPVLMAASNSSGGVMGKMISLQSIAVAAAATGMAPSEEAKL
                     FRFTLRHSIVLACAIGLITVFYAYVMPQWAP"
     gene            complement(2314..2778)
                     /locus_tag="VLE22_07115"
     CDS             complement(2314..2778)
                     /locus_tag="VLE22_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HSB14212.1"
                     /translation="MTSRERIDDFLRQKRVAIAGVSRQPRDFTRMMFNEFVRRGYDVV
                     PVNPSAGEIDGHKCFARLQEIIPPVDSVLVMTKPTAAEAVVQDCAEAGVKRVWLYRAT
                     GAGAVSRKAVELCEARSIDVIAGGCPFMFWADSSFFHRVHGVFAKLTGSYPR"
     gene            complement(2855..3922)
                     /locus_tag="VLE22_07120"
     CDS             complement(2855..3922)
                     /locus_tag="VLE22_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14213.1"
                     /translation="MRKRHILGVLVWVGMALLTLVPTANCMEIFGAWHCGNDYCTWST
                     VRNMTDFDLKNRWLIDRGDGIPSVNLVILSFVNPLKLLNDGPLNGVPVGMTQDVVNYF
                     TSRGVRVMLSIGGITYVDYWNQALAGDAYQLGLNAATAAQRLGVGIEIDYEENSNPNL
                     TGLQAFIDAYRSVHPYDITGTNPAARLTIDLAAGDRWLIDLTEHATRVWPTNTSPVLD
                     YANAMVPARQPRNAADATANWQEHIDGKPQYAPPILPLAPAKFTGSLWLVTGRKPAPE
                     CVNFNNSLTKSTGSYVQTVQPNASHPEWSRGMLGYMFWAAECQGTRSLCTTPPNTCEG
                     GMGVAATVYNIPIPMPPLRQQ"
     gene            complement(4008..5654)
                     /locus_tag="VLE22_07125"
     CDS             complement(4008..5654)
                     /locus_tag="VLE22_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB14214.1"
                     /translation="MTRRQLLMAAFAAPGPGFRLTDVTAQAGLSFRHNTGAFGAKYLP
                     ETMGSGCAFLDYDNDGWPDILLINGRDWPGHERQRSTLRLFRNNRNGTFTDVTRAAGL
                     DVEMYGMGVAVGDFNNDGFPDLYITAVGQNRLFRNNGRGRFVDVTIPAGLGSRSAFST
                     SALWFDYDRDGHLDLFVCNYVRWSPEQDVNCSLDGKSKSYCTPEAYRGATGWLFHNKG
                     DGTFEDVTAESGIFDPSSKSLGVAMLDLDNDGWPDLFVANDTQPNKLYRNNRDGTFTE
                     SAVRMGVAFSEDGKARAGMGVDTGDYQRSGRMGIAVTNFDNEMMGLYESTGRGFVDRA
                     IPAGVGAASRQRLGFGCVFADVDLDGYLDLIAVNGHIDDTVRSLGRGAAHAQVPHLFL
                     NDGKGRFRDVAVEAGREFAVPKIGRGIALGDFDRDGDLDVLITTNSGPALLFRNDVSS
                     GNQSIRFRLEGRKSNRDGIGAVVQVFDASGMQMQMVKSGSSYLSSSDKSLTFGVGSRT
                     HVDRVAIFWPSGRADEFRKLAAGKTYLCVEGENPRPQRGR"
     gene            complement(5651..6292)
                     /locus_tag="VLE22_07130"
     CDS             complement(5651..6292)
                     /locus_tag="VLE22_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB14215.1"
                     /translation="MPEERTERKIRVLLVDDHALVRLGFRRLLEDDPELIVVGEASDG
                     TEAVELARKLVPDVVVMDYAIPAPNGALATRMIRESVPSVRVLMLSMHPEPTYVANSR
                     EAGASGYLLKNAYDLELVAAVKGVLETDWLQDPRLEQASFGAGVKRRLSKRELEVLQH
                     IVSGKSNKEIATVLNLSANTVGVHRANIMDALDIHNTAELVVYAIRHGLVSLP"
     gene            complement(6285..7604)
                     /locus_tag="VLE22_07135"
     CDS             complement(6285..7604)
                     /locus_tag="VLE22_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB14216.1"
                     /translation="MESPTGRLVAGLAFTLVVIGVYAAYTLNSVSRMRDLQTRIVDRN
                     RRASLQLIRIQNDLNSLALSMRDMLDPGEGYPLAAWKAPLRRIRQNLEDAIASEAALA
                     EGDRAPTQTAFLKSSFAEFWRACDGMLAYAANGEETKAKELVRTTLQPRQEALSALTA
                     RLLVERNDQESRGGQQVAEIYSQIETTAYRFLAISIVLIVLTSVGLIRSNRSLFSQLA
                     ELAEQRRELARQLIATQESTLRAVSRDLHDEFGQVLTALGAMLRRADRQAPDSDFRRQ
                     VHEAGEIVQGALENIRSLSQSLQPVILEEQGLLAAIEWHLPVFEHQTGIRVHYQPPRG
                     NVDVPTEASIHVFRILQEALNNVARHAAVDEVDVSLDADESSFALTIEDRGRGMPDPL
                     RPGTGLAAMRERAELIGGTLEVTRGKESGTRIALRLPKEAPREAPVA"
     gene            complement(7775..9031)
                     /locus_tag="VLE22_07140"
     CDS             complement(7775..9031)
                     /locus_tag="VLE22_07140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB14217.1"
                     /translation="MKEASAARLKATSASNAPGRADEEASPKYPGWRVVAAANLGVMV
                     SFGSLLVFTFGVFLKPLAAEFGWSREEISRAFGIAAMTVAFCSPVLGRALDRFSPRPV
                     ILPCFAVFGFGVLALSQLSGSLWQLYGIFLVLGIVGNGTTQMGYSGAVSSWFMQRRGL
                     ALACVMAGVGIGSILHPILAQWCIGMYGWRTAYVLLGSLVLVLGLPLTALWVRRRPDG
                     HAGSYDFEGLTTAAAFRQREFWLLVATLFLSSMTANGVLTHLAAHLSDRGMPAGSAAF
                     TTGLLGGANLAGRLATGWFLDRFSGPRLSMCLLVLMAAGMWILSRADSLPVAAVSAIL
                     IGIGLGGEADITPYLLSRYFGLRSFSTLYGFTWTFYAMAGAAGPVLFGHVFDSTGSYS
                     TLLGFAGAMTLVAASLMLWMRRYRIL"
     gene            complement(8985..9329)
                     /locus_tag="VLE22_07145"
     CDS             complement(8985..9329)
                     /locus_tag="VLE22_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14218.1"
                     /translation="MKSHAAVAAELLGNRALVEEVIADLESSSLPPAEKALLRFVDKV
                     NRDSPNISAGDMKPLYEAGWTDEQIYYAITVCALFNFYNRWIDATGVHALSEEAHREG
                     GKRSAAQGYVRK"
     gene            complement(9330..9572)
                     /locus_tag="VLE22_07150"
     CDS             complement(9330..9572)
                     /locus_tag="VLE22_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14219.1"
                     /translation="MFLPDVEANPQPSPWATVIEGMQKLGREYPQIWHLFAFKPSATE
                     HLARFTQEILREEAPLTPGLRELIAAYTSGRNHCPF"
BASE COUNT         1875 a         3378 c         3033 g         1975 t
ORIGIN      
        1 cgcaagggag cggttagctg cgtcacgtca aaggccttcc gcgtcgaaca gtgccatggg
       61 ctcgccctgc tcgtcgatta cgtactgaag agcatgcctg acatcctcgt ccttccacag
      121 ccaacgggtg cttccatgtc gcgcccagcg tttccgatcc ggtccgtcgc ttgtcaaccg
      181 gttcagctcg cggctggcgt acgatttgaa ctcgttcatg attctctcgg gtcgagtctc
      241 ggcctccaca atggtgtgca cgtggttgga cctgacgtgt gcagccagca ggttccagcc
      301 gcggtacgcg caatgcctct ggatggccgc cagtaccact gtccggcccg cttcgttgag
      361 cacataggga tactggagca tttctcgacg ttctgcgatg acgcgttgtg catcgggctc
      421 taatagccgg ctgccaacca aattgtgacg ccgatccacc gatcccgatt cctcgccgcg
      481 caagcgcgcg ccgtagcagg cgaacgtaat gaaatacctc aaccaccgct cccttgcggt
      541 cgcggctcgg cctggcgtac cactacgctc ggatttcctc gattcgccgg cactgctaag
      601 aatctatcac tttcatgtcg tgcacccctc agcggcggac aacttgacat tgcgggtgag
      661 catttattat caaggtcact catcatgtgg gagcaacact acactcctgt tggcggtagt
      721 ctcgcgtggt ccacgctcgt tgccgccttg cccatctttg cgctgctcta cctgctgggc
      781 gtcaggcgaa agcccgcgtg gaaagccgct ctgttcggcc ttgttgcggc cgcggtggta
      841 gcgctggtcg cttaccggat gcccgtggac aagatgctga gcgcggtcgg cttcggagca
      901 gccttcggcc tgttcccgat cggttgggtg gtcttctgcg ccattcttct ttaccgtgtg
      961 acgctcgaga cgggcaagtt cgagatcgtt aaggactccg tgggcagcct gaccgacgac
     1021 cgccgcctcc aggcattgct gatcgctttc gccttcggcg cattcatcga gggagcggcc
     1081 ggcttcggca cccccgtggc agtcgctgcg gcgatgctga ccggtttggg tttctccccg
     1141 ttctatgccg ccggcatctg tctcctggcg aacaccgcgc cggtggcctt cggctcgatc
     1201 ggaacgccga tcctgacgct gaacgccgtg accggccttc ctctcgaggg tctcagcgcg
     1261 gccgtcggcc gcatctgcgc gccggtctcg ctgatcgttc ctgcgtacct gatcgccgta
     1321 atgggcggat ggaaagccat gaagggcgtg ctgccggccg tgatcgcctg cggcgtgtcg
     1381 ttcgccggca cccagatcct tgtttccaac tacctcggcc cgcagctcac cgatattctg
     1441 agttccctgg ccgcgctcgg ctcgctggtg ctcctgtttc tcgtttggaa gccgaaagac
     1501 cggtacgcgc tcgaggggga ccacgtgatg gacgtccacc ggaagcatca ctcggcgcgg
     1561 gagttgtgtc tggcgtgggg gccttacgga atcctcgttg tgtttgtgct gctctgggga
     1621 ctgtacaagg ccccgctgaa taaagtgacg ctcctgttca actggcccgg tctgcatgag
     1681 caggtcatcc agatggcccc tgtggtcgcg aatccgtcgc cgtacaaggc cgtctaccgg
     1741 ttggagtggc tggcggctcc ggggaccgcc tgcctgttcg cggcgatcct ttccgccatg
     1801 ctgctcggca tgtcaccccg gcagttcggg cgtgtgttcg ccgggaccgt gaaacagttg
     1861 cttttgtcgg agctgacgat cgcctcggtg ctggccctcg ccttcctgat gaattactcg
     1921 ggagcgaccg caaccctcgg gctggctttc gcagccaccg gagtgctatt ccccttcttc
     1981 agttcgatgc tcgggtggct cggcgtcttc ctgacgggct cggatacctc ggccaacgcg
     2041 ctcttcggga accttcaggt cgtgacggct gggcggctgg atctgagccc cgtgctgatg
     2101 gctgcatcga attcgagcgg cggcgtgatg gggaagatga tcagtctgca aagcatcgcg
     2161 gtggccgcgg ccgcgacagg gatggctcct tcggaagagg cgaagctgtt ccgtttcacc
     2221 ctgcggcaca gcatcgtgct cgcctgcgcc atcgggctga tcacggtctt ctacgcctac
     2281 gtgatgccgc agtgggcgcc gtagggcgaa gcactatcgt ggatagctgc cggtcagctt
     2341 cgcgaagacg ccgtgtacgc ggtggaagaa gctggaatcc gcccagaaca tgaacgggca
     2401 gccgcccgcg atcacgtcaa tgctccgcgc ttcgcagagt tcgacggcct tgcggctgac
     2461 cgcgcccgcg cccgtggcac ggtacagcca cacccgtttc acccccgctt ccgcgcagtc
     2521 ctggaccacg gcttcggcgg cggtgggctt cgtcatcacg aggacggagt ccacgggcgg
     2581 aatgatctcc tgaaggcgtg cgaagcactt gtggccgtca atttcgccgg cggacggatt
     2641 caccggcacg acatcgtagc cgcgccgcac gaactcgttg aacatcatcc gggtgaaatc
     2701 gcgtggctgc ctggagacgc cggcaatcgc aacgcgcttc tgccgcagaa aatcgtcgat
     2761 ccgctcacgg gacgtcatcg attctggacc caagccagag taccataacc gtggcacagg
     2821 accttagcca gactctagcc tgggatgccc gccatcactg ctgccgtagc ggcggcatcg
     2881 gaatcgggat gttgtagacc gtggcggcca cgcccatgcc gccctcgcag gtgttgggcg
     2941 gcgttgtgca aaggcttcta gtgccttgac actcggccgc ccagaacatg tagccgagca
     3001 tcccacggga ccactcgggg tggctggcat ttggctggac agtctggacg tagcttcccg
     3061 tcgacttcgt caacgagttg ttgaagttca cgcactcggg cgcaggtttc cggccggtga
     3121 ccagccagag gctcccggtg aacttggctg gcgcgagcgg aaggatcggc ggcgcgtact
     3181 gcggtttgcc gtcgatgtgt tcctgccagt tcgcggtcgc gtcggccgcg ttcctcggct
     3241 ggcgtgcggg aaccatggcg ttagcgtaat caaggacggg gcttgtgttc gtaggccaca
     3301 ctcgcgtggc gtgctcggtc aggtcgatga gccatcggtc gccggctgcc aggtcgatgg
     3361 tgagacgggc cgccggattg gtgccggtaa tgtcgtatgg atgcaccgaa cggtatgcgt
     3421 caatgaacgc ctgcaagccg gtgaggttgg gattcgagtt ctcctcgtaa tcgatctcga
     3481 tgccgacacc gagtcgctgg gctgcggtgg ctgcatttag cccgagctgg tacgcgtcgc
     3541 cggccagcgc ctggttccag taatcgacgt aggtgatgcc gccgatagac agcatgacgc
     3601 gaacgccacg actggtgaag tagttgacca catcctgcgt catgcccacc ggcacgccgt
     3661 tgagcggccc gtcgttgagc aacttcaagg gattgacaaa gctcaggatc accaggttca
     3721 ccgagggaat gccgtcaccg cggtcgatca gccagcggtt cttcaggtcg aagtcggtca
     3781 tgttgcgcac ggtggaccag gtgcagtagt cgtttccgca gtgccacgcg ccgaagatct
     3841 ccatgcagtt cgctgtgggg acaagcgtca gcagcgccat gcccacccag acgagaacgc
     3901 ccagtatatg ccgctttctc atagcgacct cccatgatgt cttatctact gcaaatcgat
     3961 gccaaacacc taattccgga actgctcccg tagtaactta aattcgacta gcggcccctc
     4021 tgcggccgtg gattctcccc ttccacgcac agatacgtct tgcccgcggc aagtttgcga
     4081 aactcgtcgg ctcgcccgct gggccagaag atcgccacgc gatcgacgtg tgtccgcgac
     4141 cctacgccga aggtcagcga cttgtcgctc gacgacagat agctcgatcc gctcttaacc
     4201 atctgcatct gcatgccgga cgcatcaaac acctgaacca ccgcgccaat accgtcgcga
     4261 ttcgacttcc ggccctccag ccgaaatcga atcgactgat tccccgaact cacgtcattg
     4321 cgaaaaagca acgccggccc tgagttggtg gtgatcaata cgtccaggtc gccatcacgg
     4381 tcgaagtcac cgagcgcaat gccgcggccg atcttgggaa cggcaaactc ccgcccggcc
     4441 tccacggcca catcgcgaaa ccgccccttg ccgtcgttga gaaacagatg cggaacctgc
     4501 gcatgcgccg ctcctcgccc caggcttcgc accgtgtcat cgatgtgccc attgacggcg
     4561 atcagatcga ggtacccgtc cagatccaca tcggcgaaca cgcatccaaa accgagcctc
     4621 tgtcgtgagg ccgcgcccac acccgcggga atcgcccgat ccacaaagcc gcggccggtc
     4681 gattcataca gccccatcat ctcgttgtcg aagttggtga cagcgatccc catccgcccg
     4741 gagcgttgat agtctcccgt gtcgaccccc atgcccgccc gtgctttgcc gtcctcgctg
     4801 aacgccacgc ccattcggac cgccgattcc gtaaacgtgc cgtcccggtt gttgcgatac
     4861 agcttattcg gctgcgtgtc gttggcgacg aacaggtcgg gccacccgtc gttatcgagg
     4921 tccagcatcg ccacgcccag tgattttgac gacggatcga aaatcccgct ctccgccgtg
     4981 acatcttcga atgttccgtc gcctttgttg tgaaagagcc agcccgtcgc gccccgatac
     5041 gcctcaggcg tgcagtagga cttgcttttc ccatccagcg aacaattcac atcctgttcc
     5101 ggcgaccagc gaacgtaatt gcacacgaag agatcgagat gcccgtcgcg gtcatagtcg
     5161 aaccacaggg cggacgtcga aaacgcgctc cgcgaaccca gccccgccgg aatggtgacg
     5221 tcaacgaacc gccctcgccc gttgtttcgg aacagccggt tctgtccgac ggcggtgata
     5281 tagaggtccg ggaagccatc attattgaaa tcacccaccg ccacgcccat gccgtacatc
     5341 tccacgtcga gcccagccgc gcgcgtgacg tcggtgaacg tcccattgcg gttattgcgg
     5401 aacaatcgca gcgtggagcg ctggcgctcg tgccccggcc agtcgcgtcc attgatcagc
     5461 aggatgtctg gccagccatc gttgtcgtag tcgagaaagg cgcagccgga ccccatcgtt
     5521 tccggcagat acttcgctcc gaatgcgccg gtgttgtgcc ggaaactcag ccccgcctgt
     5581 gcggtcacat cggtcagccg gaatcccggc cccggcgctg caaacgcggc catcagcagt
     5641 tgccgccgcg tcatggcagc gacaccagcc catgccggat ggcgtagacc accagctcgg
     5701 cggtgttgtg aatgtccagg gcgtccatga tgttggcgcg atgcacgccg accgtattcg
     5761 cactcaggtt gagcacggtg gcaatctctt tgttcgactt gccgctgaca atgtgctgca
     5821 acacttccag ctcgcgcttg ctcagtctcc gcttcacacc cgccccgaac gatgcctgtt
     5881 cgagacgtgg gtcctgcagc cagtccgttt cgagcacgcc tttcaccgct gccaccagct
     5941 cgaggtcgta cgcattcttc aacaggtacc cggatgcacc cgcctcgcgg ctattggcca
     6001 cataagtcgg ttcagggtgc atgcttaaca tgagaacgcg cacgctagga acggattccc
     6061 tgatcattcg tgtcgccagc gcgccattcg gcgctggaat cgcatagtcc atgaccacta
     6121 catcgggaac cagcttgcgc gccagttcca cggcttccgt gccgtcgctc gcctcgccga
     6181 ccacaatcaa ttccgggtcg tcttccaaca ggcggcggaa tcccaagcgc acgagcgcat
     6241 ggtcatccac cagcagaacc cggatcttac gttcggttct ttcttcaggc aacgggagcc
     6301 tcccgcggtg cctctttggg cagccggaga gcgatgcgcg tgccgctctc cttgcctcgc
     6361 gtgacctcca acgtcccgcc gatcagctcc gcgcgttcgc gcattgccgc caatcccgtg
     6421 ccgggacgta gcgggtcggg cattcctcgg ccgcgatctt cgatcgtcag tgcgaatgag
     6481 gactcatcgg cgtctagcga cacgtctact tcgtccaccg cggcatgccg ggccacgtta
     6541 ttcagcgcct cctgcagaat ccggaataca tggatagatg cctcggtggg gacatccacg
     6601 tttccgcggg gcggctggta atgcacacgg atgcccgtct ggtgctcaaa caccggcaga
     6661 tgccactcaa tcgccgccag cagcccttgc tcctccagga tcaccggctg taaagactgc
     6721 gataacgagc gaatgttttc gagcgcgccc tgaacgatct cgcccgcctc atgcacttgt
     6781 cgtcggaaat cagaatccgg cgcctggcgg tcggccctgc gcagcatggc gccaagcgcc
     6841 gtcagtacct gcccgaattc atcatgcaga tctcgcgaaa cggcccgcag cgtggactcc
     6901 tgcgtcgcga tcagttgccg tgccagttcg cggcgctgct ccgccagctc cgccaactgg
     6961 gaaaagagcg atcggttcga tcggatcagg ccgacactcg tcagcacgat gaggacaatt
     7021 gagattgcca gaaagcgata cgcggtcgtc tcgatttgcg agtagatctc ggccacctgc
     7081 tggccgccgc gggactcctg gtcgttcctc tccaccagca accgcgccgt cagggccgaa
     7141 agagcctcct gccggggttg caacgtggtc cgcaccagtt ccttggcctt ggtctcctcg
     7201 ccgttcgcgg catacgctaa catgccgtcg caagcgcgcc agaattccgc gaacgatgac
     7261 ttcaggaacg ccgtctgcgt gggcgcccgg tccccttccg caagcgcggc ttcgctggcg
     7321 atggcgtcct cgagattctg ccggatccgc cgcagaggcg ctttccacgc ggccagcggg
     7381 tagccctctc ctgggtcgag catgtcgcgc atggacagcg cgagcgagtt gagatcgttc
     7441 tggatgcgga tcaactgcaa agacgcgcgt cgattgcggt cgacaatgcg ggtctgcagg
     7501 tcgcgcatgc ggctcacgga attcagcgtg taggcggcgt agacgccgat caccaccagc
     7561 gtgaatgcca ggcccgcaac cagacgcccc gtcggcgatt ccatgatgac ctactgtact
     7621 tcgtcagaga tattgtgcct tatgtctcgt ggggcagacg atcggctttt gtcgtctgcc
     7681 gccctcgcag gccaccgaaa acgatggcct gcgccgccac aaaacagact ggcggacaac
     7741 tgcatttctt gcgacacact gcactagaac aagcttatag aattcgatac cgccgcatcc
     7801 agagcataag agaagccgcc accagagtca tcgccccggc gaacccaagc agggtgctat
     7861 aagatcccgt gctgtcgaac acgtgcccga acagaaccgg tccggccgcg ccggccatcg
     7921 cataaaacgt ccacgtgaag ccatacagcg tcgagaagga gcgcagcccg aaatagcggc
     7981 tcaacaggta gggggtgatg tccgcttccc cgcccaaccc gatcccaatc aggatagccg
     8041 acacagcggc tactggcagg gagtcggcgc ggctcagaat ccacataccc gcagccatta
     8101 ggaccagcag gcacatggac aatcttggcc cggagaagcg atccaggaac caaccggtcg
     8161 ccagtcgccc ggcgagatta gcgccaccca gtaaaccggt ggtgaacgcg gcgctgcccg
     8221 ccggcatccc gcggtcggaa agatgcgctg ccaggtgcgt gagcacgccg ttcgccgtca
     8281 tcgagctcag gaacaaggtc gccacgagaa gccagaactc ccgctgccgg aaagccgcgg
     8341 cggtcgtcag gccctcaaag tcgtaagagc ctgcgtggcc gtccggccgc cgtcgaaccc
     8401 acagcgccgt cagcggaagg ccgagcacca gcaccaggct gcccagcagc acgtacgctg
     8461 ttcgccagcc atacatgccg atacaccatt gcgccaggat cgggtgaagg attgatccga
     8521 tcccgacccc tgccatgacg cacgccagcg ccaggccgcg ccgttgcatg aaccagctcg
     8581 acaccgcgcc cgagtagccc atctgcgttg tgccgttccc aacgatcccc agcacaagga
     8641 aaatcccgta gagctgccag aggctgccgg aaagctggct gagcgcaagc acgccgaagc
     8701 cgaaaaccgc gaaacacggc aggatcaccg gccgcgggct gaaccggtcc agtgcgcgcc
     8761 ccaggaccgg cgagcagaac gccaccgtca tcgccgcaat cccgaacgcc cgcgagattt
     8821 cctcccggct ccatccgaat tcggccgcca gcggcttcag gaacacgccg aacgtgaaca
     8881 ccagcagcga gccgaagctc accatcacgc ccaggtttgc cgcggccacc acgcgccagc
     8941 cgggatattt cggcgaggct tcttcgtcgg ccctcccagg ggcgttactt gcggacgtag
     9001 ccttgagccg cgctgcgctt gcctccttca cggtgcgcct cctctgagag tgcatggacg
     9061 ccggtggcgt cgatccagcg attgtagaaa ttgaagaggg cgcaaacggt gatggcgtag
     9121 tagatctgct cgtcggtcca cccggcttcg tacagcggct tcatatcgcc ggcgctgatg
     9181 ttcggcgaat cgcgattcac cttgtccaca aagcgcaaca atgccttttc cgccggcggc
     9241 agagacgagc tctccaggtc ggcgatcacc tcctcgacca gcgcccggtt tcccagcagt
     9301 tccgccgcaa ctgcggcgtg ggacttcact caaaacgggc agtggttccg gccggaggta
     9361 taggccgcga taagttcgcg cagccccggc gtgagcggcg cctcttcccg cagaatctcc
     9421 tgcgtgaacc gcgccaggtg ttcggtcgcc gagggcttga acgcaaacag gtgccagatc
     9481 tgcggatact cgcggcccag tttctgcatc ccctcgatca ccgtagccca gggcgaaggt
     9541 tggggattcg cctcgacatc aggcaaaaac attggctcca taagtgaagc cagcgtagcg
     9601 atgtcattca ccccggttca atagtagaaa cccactaggg ttccgtgggg ccggccctcg
     9661 gcggcctcgc aggccgccct gcgctggacc acgcgtactc ctccgtattt ccttttcgcc
     9721 tcatgtggag cgcgcagtct tgcgcgccac gccggcagtc atgccggtgg ctggagacct
     9781 tttttctcaa ccctttacga tcttggcgtc tttgcggaaa ccccagccgc ttctgtccgc
     9841 gacgtcttct gaccgaaccg tgtcgagcac gacctcagcc aggcggggct gttgtcgaag
     9901 tggaacggga agccgtcaaa aaagggcacg ggtttcgagc agcagctcgt cgctcatcag
     9961 cactggcaca ttgacgtctc ctacctcaac atcaccggga cgtttactac ctgtgcagca
    10021 tcccggacgg cttcagccgg tacatcgtcc actgggatct gcgggagagg atggcggaag
    10081 ccgagatcaa gatcattctc cagcgggtca ggctggtccg ccggaggcga ggactcctgc
    10141 tccacaaagt gagcggtcca ggcacatttt caccaaagta gtagaatcgg aaatttcgta
    10201 gcctgtcacc aaaactttgt caccaaaact tgcggaggtt cgtccatggc acgtttcctg
    10261 a
//
LOCUS       DATIFQ010000152         6723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_435099, whole genome shotgun sequence.
ACCESSION   DATIFQ010000152 DATIFQ010000000
VERSION     DATIFQ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435099"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VLE22_07155"
     CDS             complement(<1..1584)
                     /locus_tag="VLE22_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSB14220.1"
                     /translation="MPTGAELFVGALKQLGIGEIFTLVGDHLNEVLMEASRAGLRVID
                     FRHESGVTHAADTWARLERRPSVSLVTGGPGHTNSLTGLATANAACSPLIAVSGSRAT
                     TMADRQAFQDLDQVGMAKPAVKWAGQPPNAAQIPFYLGRAYAEATAGRMGTVHLTIPV
                     DAFRTSTEAPARVPVPAANPVPGPDTRDVVKALEVLQAAERPVVIAGSGIWWAGAEAE
                     LRQFIEGTNLPVFTITMARGLIRDDHPLCMGYADPAMSKAVQRVYQEADVVLVLGKRI
                     DYRLALGGARLFPPDLQFVQVDIHPQELGINRRLDLGICADVKATLRTMLDELGGYTW
                     PALPWLDRIRTLRAEWEATLARAGEDCGAPLHAAAVHAELRKSLPQGVLFSWDGADFG
                     HWGRAIIPALMPGGWVRLGPLGTIGAALPNVIALQLANPGKPVALVTGDGSLGFYLAE
                     LDTMVRHNLPVVIIVGNDGAWGLERELQNEFFPDRPTVGCELRRTRYDIIMKGFGGEG
                     ENIETIDEIGPAVRRAFASG"
     gene            1627..2604
                     /gene="galE"
                     /locus_tag="VLE22_07160"
     CDS             1627..2604
                     /gene="galE"
                     /locus_tag="VLE22_07160"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HSB14221.1"
                     /translation="MSRILVTGGAGYIGSHTVHLLIRSGYDPVVADNLSRGHRHNVDA
                     KRLRVLDLTDTDGLEQLMRSEGVQAVIHFAAFMAVGESVREPELYFSNNVGGSLSLFT
                     AMQRAEVNRLVFSSTAAYYGNPERVPIPEDAPAAPVSPYGESKVIVEKILRWLDQYRG
                     LRGISLRYFNASGADPEAGLGEEHDPETHLIPLLLRAVATGQPITIFGNDYATPDGTC
                     IRDYIHVSDLAGAHIVALESLLASDPSGAFNVGTGKGQSVLEVLRAVEEVTGRKVPHT
                     IGPRRAGDPPSLVADSTKLRRTLGWEPRYTEIRDIVATAWEFQRKNREL"
     gene            2725..4008
                     /locus_tag="VLE22_07165"
     CDS             2725..4008
                     /locus_tag="VLE22_07165"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HSB14222.1"
                     /translation="MKGLPMFLVAMAAFAQADYDLLLKGGHVIDPKNGINARRDVAVK
                     DGRVAEVAANIAASKALKVVDVSGLYVTPGLVDIHTHVFAGDGPAYTGENSVFPDSHS
                     FRSGVTTVVDAGGAGWRNFADFKRRIIDKSHTRVLSMLNIVGSGMAGEEKVEQDTTDM
                     DAKATAEAAKQNREFVVGIKTAHFRAPEWTAVDRAVEAGKLAGMPVMVDFGAFTSERP
                     FEDLVLKKLRAGDIYTHTYLAAVPMLDDQGIVRPYLFEARKRGVIFDAGHGAGSFVFR
                     QAVPAIRQGFLADSISTDLHINSMNAGMKDMLNVMSKFLNMGVALDDVILRSTWNPAR
                     EIRREELGHLSVGAVADIAVLRLQKGSFGFVDVHGARMKGTEKLVAELTVRDGRVVWD
                     LNGITREDWDKLGNYRPQGDPRWDATLGRSVRSRR"
     gene            complement(4031..5848)
                     /locus_tag="VLE22_07170"
     CDS             complement(4031..5848)
                     /locus_tag="VLE22_07170"
                     /inference="COORDINATES: protein motif:HMM:NF021375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HSB14223.1"
                     /translation="MAILRTALSQPFAGDWHLLLEYPIPRRGKRIDAVLLAKDLILVI
                     EFKCGARQYDRVAHVQVEDYCLDLRDFHRESRNRTLVPVLVATNAKEQIPPSGEVMDS
                     VAPVWLANAHSVTQVLEQVATKYQSPNSEPIDASRWNAAEYVPTPTIVEAARVLYEGQ
                     NVREISRCHAGAENLTKTSAAVMLAIQRARHEEHKLICFVTGVPGAGKTLAGLNIVHN
                     RELHEGSLGVFLSGNGPLVKVLSEALARDDCERARHSIAESRREVSTFIQNVHRFIDA
                     HFASSSPPQDRVVIFDEAQRAWNREQSLRKFKRDRSEPEIMLDIMNRHDGWAVIVALV
                     GSGQEINTGEAGLAEWGRALAARSRHWNVLVSPHAVNGDPANNGDLLFETPPRGIHLQ
                     EDAALHLSVSLRSYKAHAVSEFVAHLLARHPEDARSVLASCPEFPIVMTRDFERARGW
                     LRCRQRGSRRTGLIASSGGRRLKAHGLDVRSELDVENWFLNPSSDVRSSYYLETPATE
                     FGIQGLELDWTGLCWDIDLVPDSADWQIQAFKGTRWQTVRDATRRSYVLNKYRVLLTR
                     AREGMVIWVPRGNSADETRPASAYESIAEYLALCGITAI"
     gene            complement(6288..>6723)
                     /locus_tag="VLE22_07175"
     CDS             complement(6288..>6723)
                     /locus_tag="VLE22_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14224.1"
                     /translation="LHPVLNAIVGGWQTSHLLMLSSGANLRFGGMLTDGSSPKLEDRT
                     PQKMFDTSKFKQLPPYTRRENPWSYPGVNGHKNWNLDSTLTKTFPIKERLDMEFRFEM
                     YNSPNGFVWADPNMTVTSSLFGRSTNQVNRGRECQYSLRLLF"
BASE COUNT         1257 a         2217 c         1984 g         1265 t
ORIGIN      
        1 tcccgacgcg aatgcccttc gcactgccgg gccgatctca tcgatcgtct cgatgttctc
       61 tccctcaccg ccgaagccct tcatgatgat gtcgtagcgc gtccggcgca gttcgcagcc
      121 caccgtcggc cggtccggaa agaactcgtt ctggagttcc cgctcgagcc cccaggcgcc
      181 gtcatttccg acgatgatca ccaccggcag attgtggcgc accatcgtgt ccagctctgc
      241 aagatagaaa cccagagatc cgtcgccggt aacgagcgcc accggcttgc ccggattcgc
      301 cagttgcagc gcgatcacgt tcggcagcgc ggcgccgatc gttccgagcg gccccagacg
      361 gacccacccg ccaggcatga gtgccggaat gatcgcccgt ccccagtgcc cgaagtcggc
      421 tccgtcccac gagaacagga caccctgcgg caacgatttc cgaagctccg catgcacggc
      481 ggcggcatgc agtggcgcgc cgcagtcttc tcccgcacgt gcgagcgtcg cctcccactc
      541 cgcccgcagc gtccggatcc ggtcgagcca cggcaaggcg ggccaagtgt acccgccgag
      601 ttcatccagc atggtgcgta gcgtagcctt cacgtccgcg cagatgccta gatcgagccg
      661 ccggtttatt cccaactcct gtgggtgaat gtccacctgg acgaactgca agtccggcgg
      721 aaagagcctc gctcctccga gcgccaaccg gtagtcgatg cgcttgccca ggaccagcac
      781 cacatccgcc tcctggtaca cgcgctgcac ggctttgctc atcgccgggt cggcgtagcc
      841 catgcacaac gggtggtcgt ctcggatcag cccgcgcgcc atggtgatcg tgaagaccgg
      901 cagattcgtt ccttcgatga actggcgaag ctccgcctcc gcgccggccc accagatccc
      961 actgccggca atcaccaccg gacgctcggc cgcctggagc acctcgaggg ccttcacgac
     1021 gtcccgcgtg tccggaccgg ggacgggatt cgcggcaggt accggaacac gcgccggggc
     1081 ttccgtgctc gtccggaatg cgtctaccgg aatcgtcaga tgaaccgtgc ccatcctccc
     1141 cgccgtcgcc tccgcatagg cccgcccgag gtagaacggg atttgcgccg cattgggcgg
     1201 ctgcccggcc cacttcaccg ccggcttcgc cattccaacc tggtcgagat cctggaaggc
     1261 ctgccggtcg gccatggtcg tcgccctgct gccgctcacg gcaatcagcg gactgcacgc
     1321 tgcgttcgcc gtcgcgaggc cggtcagcga gttcgtatgg cccgggcctc cggtcaccag
     1381 cgacaccgac ggccgccgct cgagcctcgc ccatgtgtcg gccgcgtggg tcactccgga
     1441 ttcgtgccgg aaatcgatga cccggagccc ggcacgcgac gcttccatga gcacttcgtt
     1501 caggtggtct ccgaccagcg tgaagatctc gccgatgcct agctgcttca gcgcgccgac
     1561 aaatagttcc gctccggttg gcattgtgct gccatcctac tacaatcact tctttaccct
     1621 cccgctatgt cgagaattct ggtaaccggc ggcgccggat acatcggctc tcacaccgtc
     1681 catctgttga tccggagcgg ttacgatccg gtggtcgccg acaacctgtc ccgcggccac
     1741 cgccacaacg tggatgcaaa gcgcctgcgg gtactcgacc taaccgacac cgacggtctc
     1801 gaacaactga tgcgtagcga aggcgtgcag gcggtgatcc atttcgccgc tttcatggcc
     1861 gtcggcgagt cggtccgtga gccggagctg tacttctcga ataacgtcgg cggctcgttg
     1921 tcgctgttca ccgccatgca gcgcgccgaa gtgaaccggc ttgtcttttc ttccaccgcg
     1981 gcttactacg gaaatccgga gcgggttcca atccccgagg acgcaccggc ggcgccggta
     2041 agcccgtacg gcgagtccaa agtgatagtg gagaagatac tgaggtggct cgatcaatac
     2101 cgcggtctgc gcggcatttc cttgcggtac ttcaacgcca gcggcgctga tccggaggcc
     2161 ggcctcggcg aggagcacga ccccgaaacc cacctcattc cgctgctgct ccgcgccgtc
     2221 gctacggggc agcccatcac gattttcggt aacgattacg ccacgccgga cggcacgtgc
     2281 atccgtgact acatccatgt cagcgacctc gccggcgcgc acatcgtggc gctggagtct
     2341 cttctcgcca gtgatccctc cggagcgttc aacgtgggca ccggaaaagg acagtccgtg
     2401 ctcgaggtgt tgcgcgcggt ggaagaagtg accggtcgaa aagtcccgca cacgatcggc
     2461 ccgcgccggg ccggcgaccc gccctcgctc gtagccgatt ccacgaagct ccgtcgcacc
     2521 ttgggctggg agccccgata caccgagatc cgcgacatcg tcgccaccgc ctgggagttc
     2581 cagagaaaga accgcgagct ttagccccac ctgggacagg cccactctgc cctgagcgtt
     2641 gtgtgcgagc gggcagagct gcctgtccca cctcccaagc ccctgccgcg cgcgtggacc
     2701 cgcttcttcg ctagaatgtc gcgaatgaaa ggtctcccga tgttcctcgt ggctatggct
     2761 gcttttgcgc aggcggacta cgacctcctg ctcaagggcg ggcacgtgat cgacccgaag
     2821 aacggcatca acgcccggcg tgacgtggcc gttaaggatg gcagagtcgc cgaagtggct
     2881 gccaacatcg ctgcgtcgaa agcgctgaag gtggtggatg tctcaggtct ctacgtgacg
     2941 cccggcttgg tcgatatcca cactcacgtc ttcgccggag acggccctgc ctatacgggc
     3001 gagaacagcg tcttcccaga cagccacagc ttccgcagcg gagtgactac cgtggtggac
     3061 gccggcggcg ccggttggcg caacttcgcc gacttcaagc ggcgcatcat cgacaagtct
     3121 cacacccggg tgctctcgat gttgaacatc gtggggagcg gcatggccgg cgaagagaag
     3181 gtcgagcagg ataccaccga catggacgca aaggccacgg cggaggcagc gaagcagaat
     3241 cgggaatttg tggttggcat caagaccgcg cacttccgtg caccggagtg gaccgccgtc
     3301 gatcgcgccg tcgaggctgg caaactggct ggcatgcccg tgatggtgga cttcggcgca
     3361 ttcacgtccg agcggccttt cgaagacctc gtgctaaaga aactccgtgc cggcgatatt
     3421 tacacccaca cctacctcgc tgcggttccg atgctcgacg atcaaggcat agtccgcccc
     3481 tacctcttcg aagcacgcaa gcgcggcgtc atcttcgacg ccggccacgg cgccggcagc
     3541 ttcgtgttcc gtcaggccgt tcccgccatc cggcagggat tcctcgccga ttcgatttcc
     3601 accgacctgc acatcaatag catgaacgcc ggcatgaagg acatgctgaa cgtgatgtcg
     3661 aagttcctga atatgggagt tgctctcgac gacgttattc tccgctccac ctggaacccg
     3721 gctcgcgaga tccgccgtga ggagctcggg catctctcgg tgggcgcggt ggccgacatc
     3781 gcggtcctgc gcctgcaaaa aggctcattc ggcttcgtgg acgtccacgg cgctcggatg
     3841 aagggaactg aaaagctggt cgccgagttg accgtccgcg atggccgcgt agtctgggac
     3901 ctcaacggca tcacccgtga ggactgggac aagctcggca actatcggcc ccaaggcgac
     3961 ccccgttggg acgccaccct cggccgctct gtccgctcgc gtaggtaagg ttgggccagc
     4021 gcctccgcat tcagatcgcc gttatgccgc acaacgcgag gtactccgca atcgactcgt
     4081 aggctgaagc cggccgcgtc tcatcagccg agttgccccg cggcacccag atgaccattc
     4141 cctcacgggc tcgagtcagc aaaactcggt acttgttcag cacgtagctg cgccttgtgg
     4201 cgtcgcgcac ggtttgccac cgcgttccct taaaggcctg gatttgccag tcagcactat
     4261 cgggcacaag gtcgatgtcc cagcaaagtc cggtccagtc caactcgagt ccctgaatgc
     4321 caaactctgt tgccggtgtc tcaagatagt aggacgagcg aacatcggaa cttggattca
     4381 ggaaccagtt ctcaacgtcc agttctgagc gaacgtcgag gccgtgggct ttcaaccggc
     4441 ggccgccgct gctcgcaatc aagccggttc ttcgcgagcc gcgctgtcgg caccttagcc
     4501 acccccgtgc ccgctcgaag tcacgggtca tcacgatagg gaattcgggg caagaagcca
     4561 gaaccgatcg agcatcctcg gggtgccgtg ccagcaggtg cgcaacaaac tctgacacag
     4621 catgggcctt gtaggaccgc aacgagacgc tcagatgtag cgcggcgtcc tcctggaggt
     4681 ggattcctct gggtggtgtc tcgaagagca ggtctccatt attcgctggg tcgccgttga
     4741 ccgcgtgtgg agatacaagc acattccaat gccgggaacg agccgccaag gctctccccc
     4801 actcagcgag gcccgcttct ccggtgttga tttcttgccc gctgccaact aatgccacga
     4861 ttactgccca gccatcatgc cgattcatga tgtctaacat gatctccggc tcagaccggt
     4921 cgcgcttgaa tttcctcaga gactgctcac gattccaggc ccgttgcgct tcatcaaata
     4981 tgacaacccg atcttgcggt ggacttgacg aggcgaagtg cgcgtcgatg aagcggtgta
     5041 cgttctgaat gaacgtcgaa acctcgcggc gcgattcggc gatcgaatgc cgggcccgtt
     5101 cgcaatcgtc gcgagcaagt gcctccgaga gaactttgac gagagggcca ttgcccgaca
     5161 gaaacacacc cagcgaacct tcgtgcaact ctctgttgtg aacgatattc agcccggcga
     5221 gggtcttgcc agcacccggc acacctgtca cgaagcagat gagcttatgc tcctcgtggc
     5281 gcgcacgttg gatggctaac atgaccgccg cactcgtttt cgtgaggttc tctgcacccg
     5341 cgtggcatcg cgaaatctca cgaacatttt ggccctcata gaggacccga gctgcctcca
     5401 cgatggtcgg cgtcggaacg tactccgctg cgttccaccg actagcatcg ataggctccg
     5461 aattcggcga ttggtacttg gtggctacct gttcgagcac ctgagtgacc gaatgcgcgt
     5521 tagcaagcca gacgggagct acggaatcca ttacctcgcc tgaaggtggt atctgttctt
     5581 tggcgttcgt cgcgaccagg acgggaacca acgttcggtt gcggctttct cggtggaagt
     5641 cccgcagatc caaacaatag tcctccacct gaacatgagc aacacggtca tactgtcgag
     5701 cgccgcactt gaactcgatg accaagatca gatccttggc gagaaggacg gcgtcaatcc
     5761 tttttccgcg cctcgggatc gggtactcca acagcaaatg ccagtctcca gcgaaaggct
     5821 ggctcagcgc cgtgcggagg attgccacct gttggcgcca agcttctagc tgttcagcgg
     5881 tgagttcggt caagcctaac cttgtcaccg cggactggcg gccgatgatg aaatcctctt
     5941 ggtgttctaa gaattctgtg atcgaggccc gatagaaagc cgacattccc tccagtctat
     6001 gcgcacggca aagaaaatag agtgggaata tccccttttc acggcgcaga ctctaagttc
     6061 gcgctcggca actatcggcc ccagggcgac ccccgctggg acgccaccct cggccgctcc
     6121 gtccgagcgc gaaagtaagg tcgggcgagt ctagccacga gagtcgtcgc ttcgagcccc
     6181 aatagcgcgc ccgatgccca ggaaccggaa cggcccagat agggaatcgg gcggggtggg
     6241 ccaatgccat cgcgagggct tgaagcctgc ccacccccag cggcgcgtca gaacaacagc
     6301 cgcagcgagt actggcactc gcgtccgcgg ttcacctggt tggtgctccg gccgaacagc
     6361 gagctggtga cagtcatgtt cggatctgcc cagacgaagc cgttaggact gttgtacatc
     6421 tcaaagcgga actccatgtc caagcgttcc ttaatcggga aggtcttggt cagcgtcgag
     6481 tcgagattcc agttcttgtg gccatttacc cccggataac tccacgggtt ctcccgcctg
     6541 gtgtacgggg gcagttgctt gaacttcgag gtgtcgaaca tcttctgcgg agtcctgtcc
     6601 tccagtttcg ggcttgaacc gtccgtgagc atccccccga accgcaggtt tgcgccggag
     6661 ctcaacatca gaaggtggct ggtctgccat ccacccacga tcgcgttcag caccgggtgc
     6721 agg
//
LOCUS       DATIFQ010000153        12661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1775459, whole genome shotgun sequence.
ACCESSION   DATIFQ010000153 DATIFQ010000000
VERSION     DATIFQ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12661
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775459"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..752)
                     /locus_tag="VLE22_07180"
     CDS             complement(255..752)
                     /locus_tag="VLE22_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14225.1"
                     /translation="MAQVESKSTGKILSLLQEPAGLPYFVTSIAEREGMRLPTIEPRQ
                     VIPQNITPEAVERSAGATYPAIYVYCEKLANSLREKFRTFSGTARMAVEVRVSQDRLE
                     GLERKLSLYVEAVTDVLDARRGEWGQGLFYTGGYEVAFGPTKHGGKNFIQTAKVGFDV
                     QVSVG"
     gene            complement(757..1524)
                     /locus_tag="VLE22_07185"
     CDS             complement(757..1524)
                     /locus_tag="VLE22_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14226.1"
                     /translation="MALLSDGTISTLGDLRDYESAIFELASTEQIDLSRKLALAKQEI
                     EVELTDFLLRQSMHQADGSRGARPDLGRVVVTPALRQWHTYHALALSYRDAYSTHYND
                     RYLGKWKGYERLASEASRKSFDIGIGLVYDPIPKAAPARLSAVAGSSPGGTYWVRVAW
                     TGAAGQEGEPSDPVYLTVTEGSALAIEATDAPVNATGWNVYAGITSDSVELQTATPMP
                     AGTNWVMPPDGIIRGRQAGEGQPAAYFVTLRRVLQRG"
     gene            complement(1558..1884)
                     /locus_tag="VLE22_07190"
     CDS             complement(1558..1884)
                     /locus_tag="VLE22_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14227.1"
                     /translation="MDLKAFYQKLRKIETEIIEPFVIIASKETPDGGRPGVLTEVGRR
                     IAARMIAEGVASLAEEAEVSRFRAEISAARREAEQQAIASKLQVTVVTEGKSKKAAGQ
                     QAGQRD"
     gene            complement(1942..2853)
                     /locus_tag="VLE22_07195"
     CDS             complement(1942..2853)
                     /locus_tag="VLE22_07195"
                     /inference="COORDINATES: protein motif:HMM:NF023081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HSB14228.1"
                     /translation="MPAVTSANVANAIVKLVAVDALPALMGNLVMGNLVNRDFEPTLG
                     QAGDTVNVPIPPVLVANNLAEGGTVQAQNPDLGNAQIVLNTHAEATFQIPDVTKVLAV
                     PDLLRLYMQPAVVALAEKIESDLLSTYASFTANAPVGTPGMAITEAIVDGAETALFQS
                     KVPASESKYLVVDAATYSQLRQIPRFSEFQTAGEAGLRALVDGTVGKIKDFFVFRSQF
                     VQKTGSAPVTTNNLGFARSALGLVMRRLPQPLPGTGAIAEYAELGNFGMRVVMSYQPN
                     TLAQQFTVDVLYGVGVLRNSFGIQVRS"
     gene            complement(2940..3527)
                     /locus_tag="VLE22_07200"
     CDS             complement(2940..3527)
                     /locus_tag="VLE22_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14229.1"
                     /translation="MEEMRNDAPVTAEAGGIEIRSLIREAIEEYSKKEAAKAEPAYKA
                     ELLEERKRRESLERRVNELISENTRSRQAAEEAERNATVRTELQRLGVVKVDLAFRAV
                     KDDIRRAEDGRLVASAEGGQVALRDYLTQFVSDNPEFLPARNLGGSGVTAARTASSGG
                     PVVDLDKIKPGMSADELEKVRREVARIASQSLGGM"
     gene            complement(3515..4936)
                     /locus_tag="VLE22_07205"
     CDS             complement(3515..4936)
                     /locus_tag="VLE22_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14230.1"
                     /translation="MFNIDREHPEYAAKRAMWRTYRDLYAGGEQFKEHASEYLVRRQK
                     EPLDVFGERLSRVFYENYLGSIIDWYAATLFRREPLLSFHGNNEAAREFFAKFVEDCD
                     LRGTSLTDFFRRVLIEALVTGSGYVLVDFPRPSGPVTNRAEEEAAGWARAYLVDCRAE
                     ELINWSHDGNGNLDWVVVRTSHLRQERESDAPWVRETRWVYYDKELFRTYRRTERGDE
                     PGAPELVDEGRHGLAKLKRVPIFELKVSEGLWLANKAAMLQLEHFNKSNALSWALTMG
                     LFATPVVYSDREWNQIAGESYYIQLGSEDRFGWTEPEGHVYQIAADNLTRLKDEIYRV
                     CYLLTQAGGALTGGSVQSGLSKQRDFTITQEVLRAYGDTVKDTLRRILCSIEAAREDD
                     LSVEVSGLDEFDIGDFSGELEDATKLLGLGVGSRTLRKQVLKKLSLKYLCDVRQEIKD
                     RIAREIDEWCEQPESQGEERWKR"
     gene            complement(4982..6283)
                     /locus_tag="VLE22_07210"
     CDS             complement(4982..6283)
                     /locus_tag="VLE22_07210"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HSB14231.1"
                     /translation="MGGAGREGTRIRDIKYSPLPSQARFHRSSARFKGFSGPVGSGKS
                     QALCQEAIRLSYLNPGRSGLLGAPTYPMLRDATQSTLFDILGRSAIPHDYLKAENVLV
                     MKDTGSRIIFRPVDDYERLRGTNLAWFGLDELTYTAQEAWLRLEGRLRDPKAQRLCGF
                     AVWTPKGFDWVYRRFIDNTVEGYEVVLARPYENRHLLEKVPDYYERLKRSYDDRFFEQ
                     EALGKYLNVQDGQAYHAFDRSRNLAPGEMDPGLPLRWALDFNVDPMSSVIAQIRGDVI
                     HVIDEIVLRRSCTPELVEVFTSRYRFHRAGLYIYGDATGQKRQTTGSADYEMVREGLR
                     RAGHEFHFRVPKSNPSVRDRVSWVNAMLRSAGDETRLLIDPKCRELILDLEQVSFKPG
                     SENIDKEKDPQRTHLSDALGYLVCQECRPVEPAGEKGRRLF"
     gene            complement(6241..6513)
                     /locus_tag="VLE22_07215"
     CDS             complement(6241..6513)
                     /locus_tag="VLE22_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14232.1"
                     /translation="MSTSVAKTNEKEQKKKQTDKPGRKRPVKRSRLVGRVLRKFEAKL
                     QEDQYKVSVGDFVRLVQLEQEMKEDTPKEVRVTWVEPAEKEPESGT"
     gene            7014..8750
                     /gene="ilvB"
                     /locus_tag="VLE22_07220"
     CDS             7014..8750
                     /gene="ilvB"
                     /locus_tag="VLE22_07220"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HSB14233.1"
                     /translation="MSTLMSGARILLECLVREGADCIFGYPGGVMLPLYDVMYDHPIR
                     HVLVRHEQNAAFAAEGYARSTGRVGVCAATSGPGATNLVTGLVNAMMDSIPIVAITGQ
                     VTSKLIGTDAFQEADTFGITRSCTKHNYLIKNLADLPQIVHEAFYIASSGRPGPVLID
                     IPKDIFQGQAHYSPVSSIHLPGYRVFTEGHSGQIRRAAQMMWEAKRPFVYAGGGIIAS
                     EAAGELRDLVEILDAPAVCTLMGLGGLPSEHPNFISMPGMHGSYAANMGMTQTDLLIA
                     LGVRFDDRVTGRLSSFAPHAKVIHVDIDPAEIGKNRAADLPIVGDVKRVLEKLNDLLR
                     ELKPSMGDLNAAGRQAWWRQVRDWQQEHPITPVFSDSEIKPQHLMTEIDRISRGEAII
                     TSDVGQHQMWGAQFLRFNKPRLWLSSGGLGSMGFGLPAAIGAQFACPEKLVLAVVGDG
                     GFQMSVPELATIVNHGLPIKIIVMNNGYLGMVRQWQELFYNNRLCAVELESFPDAEKL
                     AAAYGFKGRTVESPRELRPALDEALAEPGPYLLNVRVSPYENVYPMVPAGAALNEMVL
                     TPPQPVPIPVPK"
     gene            8768..9325
                     /gene="ilvN"
                     /locus_tag="VLE22_07225"
     CDS             8768..9325
                     /gene="ilvN"
                     /locus_tag="VLE22_07225"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HSB14234.1"
                     /translation="MLQIISLLVENKPGALMRVTGVLTARGYNIESLTVARTLDPTLS
                     QMTIVVDVDPNLRELLVKQMNKLINVLQVNDLTGARAVAREMVLMRVRAPLEKRTSVL
                     KEAEIFGARVVDSSVEGFALEATGEQEKLEEFIDIMRSYGEFEIARSGVVVMSLRSKK
                     LRLSPPVPTKSEAERIEQNTGVTHG"
     gene            9318..10361
                     /gene="ilvC"
                     /locus_tag="VLE22_07230"
     CDS             9318..10361
                     /gene="ilvC"
                     /locus_tag="VLE22_07230"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF009940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HSB14235.1"
                     /translation="MANRYYEKDGNLNTLKGLTVAIIGYGSQGHAHALNLRDSGVSVV
                     VSDLPGSPNWKKAEAAGLRVLATAEAVKAADTIMVLVADHLQAGMYKNDIAPNLKAGK
                     TLMFAHGFNIHYGEVVPPKEVDVSMIAPKAPGHRVRELFTEGVGVPALVAIHQNPSGK
                     ALERALAYALALGCLKAGVIETTFKEETESDLFGEQAVLCGGAAELVRAGFETLVEAG
                     YAPEIAYFECLHELKLIVDLIQEGGLSYMRFSVSDTAEYGDYTRGPRVVDAHVRGVMK
                     ELLAEVQSGKFAKEWIDENKTGRKKFLAMREASRHQPIEEVGRELRAMIPFLKKRKEA
                     GVPEDQPATASGR"
     gene            10377..11987
                     /gene="cimA"
                     /locus_tag="VLE22_07235"
     CDS             10377..11987
                     /gene="cimA"
                     /locus_tag="VLE22_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HSB14236.1"
                     /translation="MKISILDTTLRDGTQGEAVSFTVEDKLMIAQKLDELGIDYIEGG
                     WPGSNPKDKEFFSRARELHLKHARLTAFGSTRFAKNPVDRDPNVRALLEAGTPVISIF
                     GKSWDLHTQRALGISEEENLKLIAETVKYLKDHGREVIYDAEHFFDGYISNPSFALRT
                     LEAARDSGADILCLCDTNGGTLTGRLTEITADVRKRFDGVIGIHAHNDTDVAVANAVA
                     AVEQGATHVQGTINGYGERCGNSNLCSIIANLELKLGHTTIGSDKLVQLTSVAKFVAE
                     LANLNLRNDQPYVGRSAFAHKGGIHVSAVLKDAATYEHVPPETVGNRQRVLLSDLSGR
                     SNILYKLKQHGLADRLTEEARRELLERIKLMEYQGYELEAAEGTFELLVREALHPGTH
                     FFDLVSLDVSTQIAGTKGTRVTATVTLKAADGVHSATASGHGPVNALDLCLRQCLSSL
                     YPTIANVRLTDYKVRVLEPKKGTAARVRVLIEWSDHRRSWTTVGVSENVIEASWQAMV
                     DALRLELMRLMEQDETIERAVEDYCWGV"
     gene            12024..12608
                     /locus_tag="VLE22_07240"
     CDS             12024..12608
                     /locus_tag="VLE22_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14237.1"
                     /translation="MRNFWKLRQPDVRRIVLVESGPRHLLEGAIPVLRRLYGESIPID
                     LVTCFAGSPVGFTAGNGRVYQVADHQGRAGRKRLYRELCALAPDALVMVCADVPIMTK
                     WKWALAWNLPAKVLILNENGDFFWFDRAHWRVILHFIAFRSGLTGGGMVRTIADFALF
                     PFTLTYLLLYAGWIHARRKVRLAMRSGAMGLDKT"
BASE COUNT         2285 a         4148 c         3769 g         2459 t
ORIGIN      
        1 ccttgcgctg caccccgtcc ggcgcctgcc ggatcgacag cttgaccgcc ggaaacctgt
       61 gcccggcatc gatcgccggc acagcgccgt aagtctgttc caccgccgtg tagaagcggt
      121 tattagtgga tgctatgtaa cacgacatga ttccccaccc catctgggat gaaatgggga
      181 ttgactacca tttcgccgcc gttgcgaaat ggctgtctgt ccccatttct tcactcccac
      241 ttttcagccc ttcttcaccc cacgctcacc tgcacgtcga acccgacctt cgccgtctgg
      301 atgaaattct ttcccccgtg cttggtcggc ccgaacgcca cctcgtaacc gccggtgtaa
      361 aacaacccct gtccccattc cccacgccgg gcgtccagca cgtccgtcac cgcctcgaca
      421 tagagggaga gcttcctctc cagaccctcc agccggtctt gggataccct gacctcgacc
      481 gccattcttg ccgtgccgga gaacgtgcgg aacttttccc gcaggctgtt cgccagcttc
      541 tcgcagtaca cataaatagc cgggtacgtc gctcccgccg agcgttcgac cgcctcgggc
      601 gtgatgttct gcgggatcac ctgtctcggc tcaatcgtcg gcagccgcat cccctcccgt
      661 tccgcgatcg acgtcacgaa atacgggagt cccgccggct cttgcagcag actcaagatc
      721 ttgcccgtgc ttttgctttc cacctgtgcc atgctgctac cctctctgca gaacccggcg
      781 aagtgtcacg aagtacgccg ccggctgtcc ctcgcctgcc tgccttcccc tgatgattcc
      841 gtccggcggc atcacccagt tggtccccgc gggcatcgga gtcgcagtct gcaattccac
      901 ggagtccgat gtgattcctg cgtaaacgtt ccagccagtc gcgttcaccg gagcgtctgt
      961 agcttcgatc gcgagcgccg agccttctgt taccgtcaga tacaccgggt cgctcggctc
     1021 cccctcctgg ccggccgctc ctgtccaagc cacgcggacc cagtaagttc cacctggcga
     1081 gcttccggcg accgcgctca gccgagccgg cgccgctttc gggatcgggt cgtaaaccag
     1141 cccgataccg atgtcaaagg acttgcggga ggcttcgctg gcgagtcgct cgtagccctt
     1201 ccacttaccg aggtaacggt cgttgtagtg ggtgctgtaa gcgtccctgt atgagagggc
     1261 aagcgcgtgg taggtgtgcc actgccgcaa tgcgggtgtc actaccaccc tgcccaagtc
     1321 cggccgcgcc ccacgggacc catccgcctg gtgcatcgac tgccgcagca ggaagtccgt
     1381 cagttccact tcgatttcct gctttgccag cgccagcttc cgcgaaaggt cgatctgctc
     1441 cgtgctggct aactcgaaga ttgcgctctc gtaatcccgc aggtctccga gcgtggatat
     1501 agtgccgtcc gacaataacg ccatcgctcc cccgtggcac aggattcagc ctgtgtccta
     1561 gtccctctgt cccgcctgct ggcccgcagc tttcttggac ttcccttcgg tgacgaccgt
     1621 cacctgcaac ttgctggcga ttgcctgctg ctccgcctcc cgcctcgccg ccgagatctc
     1681 cgcccggaag cggctcactt ccgcttcctc ggccaggctg gccactcctt cggcgatcat
     1741 ccgcgccgcg atcctgcgtc ccacctctgt cagcacgccg ggacggcccc cgtcgggagt
     1801 ctccttgctg gcgatgatca cgaacggctc gataatctcc gtctcgatct tccgcaactt
     1861 ctgataaaaa gccttcaagt ccatgactcc tccttccgca tcccttcggg ggcgaggaat
     1921 tccccgcccc cgccatctct gttacgaccg cacttggatg ccgaacgagt tccgcagcac
     1981 tcccacgccg tacagcacgt ccaccgtgaa ctgctgtgcc agtgtgttgg gctggtagct
     2041 catcaccacc cgcatgccga agttcccgag ttcggcgtac tcggcgatcg cgcccgtccc
     2101 gggtagcggc tgcggcagtc tccgcatcac cagcccgagg gcgctccgcg cgaagccgag
     2161 gttgtttgtg gtcaccggcg cgcttccggt cttctgcacg aattgcgacc ggaacacgaa
     2221 gaagtccttg atcttgccca ccgtcccgtc cacgagcgcc cgcaacccgg cctcgccggc
     2281 cgtctggaat tcgctgaatc gcgggatctg ccgcaactgc gagtaagtgg ctgcatcgac
     2341 caccaggtac ttcgactcgc tcgctggcac cttcgactgg aatagcgcgg tctcggctcc
     2401 gtcgacgatc gcttccgtga tggccatccc cggagtgccc accggcgcgt tcgccgtgaa
     2461 gctcgcgtaa gtgctgagca gatcgctctc gatcttctcg gccagtgcca ctaccgccgg
     2521 ctgcatgtac agccggagca ggtctggcac cgccaggacc ttcgtcacgt ccggaatctg
     2581 gaatgtcgcc tcggcgtggg tgttcagtac gatctgcgcg tttcccaagt ccggattctg
     2641 cgcttgtacc gttccgcctt cggcaaggtt gttggcaacc aggaccggag ggatcggcac
     2701 gttcaccgtg tcgcccgcct gccccagcgt cggctcaaaa tcgcgattga ccaggttccc
     2761 catcacgagg ttacccatca gggcgggcag agcatccacc gccactaact tgacaatcgc
     2821 attggccaca ttagctgatg taactgctgg cattgactat ctcctgtttg ttgttggttt
     2881 cgcctctgcg ggcgtttgaa ttactctctg tccggccgcc ttccacgccg cgaccgcttc
     2941 tacatgccgc caagcgactg cgaggcgatt cgtgctactt cgcgacggac cttttccagt
     3001 tcgtccgcgc tcatccccgg cttgattttg tcgaggtcca ctaccggccc gcccgaggac
     3061 gcagtccgcg ccgccgtgac gccggatccg ccgaggttcc gcgccggcag aaactcggga
     3121 ttgtcgctga caaactgcgt caggtagtcg cggagcgcca cttggccgcc ctcggccgac
     3181 gctaccaggc gtccgtcttc cgcgcggcga atatcgtcct tcaccgcccg gaatgcgagg
     3241 tctactttga ccacgcccag ccgctgaagc tcggtgcgga ccgtcgcatt cctctcggcc
     3301 tcctccgctg cctgacggct gcgcgtgttc tcgctaatca gctcgttcac gcgccgctcc
     3361 agcgactcgc gccgcttccg ctcctcgagc agttccgcct tgtaggccgg ctcggctttc
     3421 gccgcctcct tcttggagta ctcctcgatc gcctctcgta tcagcgagcg gatttcgatg
     3481 ccgcccgctt ccgccgtaac cggcgcgtcg ttcctcatct cttccatcgt tcttctcctt
     3541 ggctctcagg ctgctcgcac cactcgtcga tctctctcgc gatccggtcc ttgatttcct
     3601 gcctgacgtc gcacaggtat ttcagactca gcttcttcag cacctgcttc cgcagcgtcc
     3661 tcgaccctac ccccaggccc agcagcttcg tcgcatcctc cagctcgccg ctgaagtctc
     3721 cgatgtcgaa ctcgtccagg cccgacacct cgaccgacaa gtcgtcttcc cgcgccgcct
     3781 caatggaaca aaggattcgt cgcagcgtgt ctttcactgt gtcgccgtac gcccgcagga
     3841 cttcctgcgt gatggtgaaa tcgcgctgtt tgctcaagcc ggactgcacc gacccgcccg
     3901 tcagcgcccc tccggcttgt gtcagcaggt agcagacgcg gtagatctcg tctttgagcc
     3961 gcgtcaggtt atcggccgca atctggtaga cgtgcccctc cggctccgtc cacccgaacc
     4021 ggtcttccga ccccagttgg atgtaatacg attccccggc gatctggttc cattcgcggt
     4081 ccgagtaaac caccggagtc gcgaacaacc ccatcgtcag cgcccacgac aacgcgttcg
     4141 acttattgaa atgctccagt tgaagcatcg ccgccttgtt cgccagccac aacccctcgc
     4201 tgaccttcag ctcgaaaatc ggaacgcgtt ttagtttcgc cagcccgtgg cgcccttcgt
     4261 cgacaagttc cggggcgccg ggctcgtcgc cccgctccgt gcgccggtac gttcggaaca
     4321 actctttgtc gtagtacacc caccgagtct cccggaccca aggggcatcg ctctccctct
     4381 cctgccgaag gtgcgacgtc cgaactacta cccagtcgag attgccgttc ccgtcgtggc
     4441 tccagttgat cagctcctct gctctgcaat cgaccaggta cgctcgcgcc cagcctgccg
     4501 cttcctcctc cgcccgattc gtcaccggtc ccgacggccg cgggaagtca accagcacgt
     4561 agccggaacc ggtcaccagc gcctcgatca acacccgacg gaagaaatcc gttagcgacg
     4621 tgccccgcaa gtcgcaatct tcgacaaact tcgcgaagaa ctcgcgggct gcctcattgt
     4681 tcccgtgaaa gctaagcagc ggttcgcggc gaaacagcgt cgccgcgtac cagtcgatga
     4741 tcgagccgag gtagttctca taaaagaccc ggctcaaccg ctcgccgaaa acgtccagcg
     4801 gctctttctg gcgcctgacc agatactcgg aggcgtgttc cttgaactgc tccccgcccg
     4861 cgtagaggtc acggtaggtc ctccacatcg cccgcttcgc ggcgtactcc ggatgttctc
     4921 gatcgatgtt gaacacactc gccccctggc ggccctcgcg ccggcgctcg tcgccgcaac
     4981 gctaaaacag ccgccttcct ttttctccgg ccggctccac cggccggcat tcctggcaga
     5041 ccaggtaccc cagcgcgtcc gagaggtggg tccgttgcgg gtccttctct ttgtcgatgt
     5101 tctcgcttcc gggcttgaat gaaacctgct cgagatcgag aatcaattcc cggcacttcg
     5161 gatcgatcag caaccgcgtc tcgtcccccg ccgaccgtag catcgcgttc acccagctca
     5221 cgcggtcccg cacggacggg tttgacttcg gcacccggaa gtgaaattcg tggcccgccc
     5281 ggcgcaaccc ctcccggacc atctcgtagt ccgccgatcc ggtcgtctgc cgcttctggc
     5341 ccgtcgcatc cccgtaaatg tacaggccgg cgcgatgaaa acgatacctg ctcgtgaaga
     5401 cttccaccag ctccggcgtg cacgatcgcc gcagcacgat ttcgtcgatc acgtggatca
     5461 catcgccacg aatctgggcg atcaccgagg acatcgggtc cacgttgaag tccagcgccc
     5521 agcgcagcgg cagcccgggg tccatttctc ccggcgcgag gtttctgctc cggtcgaaag
     5581 cgtggtaggc ctgcccgtcc tggacgttta gatacttgcc cagggcttcc tgctcgaaga
     5641 aacggtcgtc ataactgcgt ttcagccgtt cgtaatagtc cggtactttc tcgagtaaat
     5701 gccggttttc gtaaggccgg gccaggacga cctcataacc ttcgacggtg ttgtcaatga
     5761 accgccggta gacccaatcg aaccccttcg gcgtccacac cgcgaatccg catagccgct
     5821 gcgcctttgg atctctcaac cgcccttcca gccgcagcca cgcttcctgc gcggtgtatg
     5881 tcagctcgtc gagcccgaac cacgccaggt ttgtgccgcg gagccgttcg taatcgtcca
     5941 cgggccggaa gatgatccgc gaacccgtgt ccttcatcac cagaacgttc tccgccttga
     6001 gatagtcgtg cgggatcgcg cttcgcccca ggatgtcgaa caacgtgctt tgcgtcgcgt
     6061 ccctcagcat cgggtacgtc ggtgcgccca acagcccgga gcgcccgggg ttcagatagc
     6121 tgagccggat cgcctcctgg caaagggctt ggctcttacc ggagccgacg ggccccgaaa
     6181 agcctttgaa tctcgcgctg gacctgtgga aacgggcctg agaaggaagc ggggagtact
     6241 ttatgtccct gattcgggtt ccttctctgc cggctccacc catgtcactc gaacctcctt
     6301 cggcgtgtcc tctttcattt cctgttccag ttgaaccagc cggacgaaat cgcccacaga
     6361 aactttgtac tgatcctcct ggagttttgc ttcgaacttc cgcaacaccc gtccaaccag
     6421 tcgggatcgc ttcacgggac gtttgcggcc gggcttgtcg gtttgtttct ttttctgttc
     6481 tttctcgttc gtttttgcca ccgacgtgct catttccgct cctccgcgac tcagggtcgc
     6541 caaacagcga accttctctg cccagagagt agcaccaggc ctcctcccca tggccgccac
     6601 gtggaaatct atcttgttga ccataaaggc cttaaaaagc cgtaaaaact tgtgaatggg
     6661 ttcataaata caatttatga aaatgcactc ttgcattgcg ggggattgta ctgtaagctc
     6721 aagacgtgga ggcattcgca cacaggaagg ttgtatcggc tcccagcgga tacactttct
     6781 ctgcgtgcac cctcccctct gttcctgtag taggcattat tatcattatc ggggtcaccg
     6841 tattcggccc cgcctgattc ggtcctgaaa ataaaggatt taagggccac cggcggggac
     6901 gaagctcagc cggtggccct tttggtttat aggggcacaa aacggcgcga aatggtcact
     6961 tttgggtacg ttttggaccg gtttcgtgcc agatcgttta ggagatttcg accatgtcga
     7021 ccttaatgag tggcgccagg atcctgctgg agtgcctggt tcgggaaggg gcggactgca
     7081 tcttcggcta ccccggaggg gtgatgctcc ccctctacga cgtgatgtac gaccacccca
     7141 tccggcacgt tcttgtacgg cacgagcaga acgcggcgtt cgcggccgag ggctatgccc
     7201 gatccacggg ccgggttggc gtctgcgcgg cgacgtccgg gcccggcgcg acaaacctcg
     7261 tgacggggtt ggtgaacgcg atgatggatt cgattccgat cgtcgccatc accgggcagg
     7321 tgacctcaaa actgatcgga acggacgcct ttcaggaggc cgacacgttc ggcatcaccc
     7381 gctcgtgcac gaagcacaac tacctgatca agaacctggc cgacctcccg cagatcgtac
     7441 acgaggcgtt ctacatcgct tccagcggcc gtccggggcc ggtgctcatc gacatcccga
     7501 aagacatctt ccaagggcag gcgcactact cgccggtttc gtcgatccac ctgcccggat
     7561 accgggtgtt cacggagggg cacagcggcc agatccggcg cgccgcgcag atgatgtggg
     7621 aagccaagcg cccattcgtt tacgcgggcg gcggcatcat cgcgtcggaa gcagccggcg
     7681 aactccgcga cctggtcgag atcctggacg ccccggcggt gtgcaccctg atgggcttgg
     7741 gcgggcttcc gagcgagcat cccaacttca tcagcatgcc cggcatgcac ggcagctacg
     7801 ccgccaacat gggcatgaca cagaccgacc ttctgatcgc ccttggcgtc cgcttcgacg
     7861 accgtgtgac gggccgcctc tcgtccttcg cgccgcacgc caaagtgatc catgtcgaca
     7921 tcgacccggc ggagatcggc aagaatcgcg ccgccgatct gccgatcgtg ggcgatgtga
     7981 aacgcgtcct agaaaaactc aacgacctgc tgcgcgaatt gaaaccgtca atgggcgatc
     8041 tcaacgccgc aggacggcag gcgtggtggc ggcaggtccg ggactggcag caggagcatc
     8101 cgatcacgcc ggtcttttcc gactcggaga tcaagccgca gcacctgatg acggaaatcg
     8161 accggatttc gcgcggcgag gccatcatca cgtcggacgt gggccagcac cagatgtggg
     8221 gtgcccagtt cctccggttc aacaagcccc gcctgtggct cagctccggc gggctcggct
     8281 cgatgggctt cggcctgcca gcggcgatcg gagcgcagtt cgcctgtccc gaaaaactag
     8341 tgctcgcggt ggtcggggac ggcggattcc agatgtcggt gcccgagctg gcgacgatcg
     8401 tcaatcacgg gctgccgatc aagatcatcg tgatgaacaa cggctacctc ggcatggtcc
     8461 gccagtggca ggagctgttc tacaacaacc ggctctgcgc ggtggaactg gagtcgttcc
     8521 cggatgccga gaaactggcc gcggcgtacg gtttcaaggg ccgcacggtc gaaagcccgc
     8581 gcgagttgcg gccggctctt gatgaggcgc ttgcggagcc cggcccgtac ctgctcaacg
     8641 tgcgcgtgtc accgtacgaa aacgtttacc cgatggtgcc cgccggcgcg gcgctgaacg
     8701 aaatggtgct tacaccgcca cagcccgtgc ccatcccggt tccgaagtaa gaaggagaga
     8761 tccccctatg ttgcagatca tctcgctgct ggtggaaaac aagcccggcg ccctgatgcg
     8821 cgttacgggc gtcctcaccg ccaggggtta caacattgag agcctcacgg ttgcgcgcac
     8881 gctggacccg acgctctcgc agatgaccat cgtcgtcgac gtagacccaa acctccgcga
     8941 gctgctcgtc aagcagatga acaaactgat caacgtgctt caggtcaacg acctgacagg
     9001 ggcccgcgcc gtggcacgcg aaatggtgct gatgcgggtc cgggcgccgc tcgaaaagcg
     9061 gacctccgtg ctcaaggagg ccgagatttt cggcgcgcgc gtggtcgatt cctcggtgga
     9121 aggtttcgcc ctggaggcga ccggcgaaca ggagaagctc gaagagttca tcgacatcat
     9181 gcggagttac ggcgaattcg agatcgcccg gtcgggcgtg gtcgtgatgt cgctccgatc
     9241 gaagaaactg cgcctatctc cgcccgttcc aacaaaatca gaagcagaga gaatagaaca
     9301 gaacactgga gtcactcatg gctaaccgtt actacgaaaa agatgggaac ctgaatacgc
     9361 tgaaaggcct gaccgtcgcc atcatcggct atggaagcca gggccacgcg cacgcgctga
     9421 accttcggga ttcgggggtg agcgtggtgg tgtcggacct gccgggcagc cccaactgga
     9481 agaaagccga agcagccggc ttgcgggtgc tggccaccgc cgaggcggtg aaagcggccg
     9541 acacgatcat ggtgctcgtc gccgatcatc tgcaggcggg aatgtacaag aacgacatcg
     9601 ccccaaacct gaaggccggc aagacgctca tgttcgccca cggcttcaac atccattacg
     9661 gcgaggtcgt cccgccgaaa gaagtggatg tctcgatgat cgcgcccaaa gcgccgggtc
     9721 atcgggtgcg cgagctgttc accgagggcg tcggcgttcc cgcgctggtg gccattcacc
     9781 agaacccgtc cggcaaggcg ctggagcgcg cgctggcgta tgcgctggcg ctcggatgtc
     9841 tgaaggccgg cgtgatcgaa acgacgttca aggaagagac cgagagcgac ctcttcggcg
     9901 agcaagccgt gctgtgcggc ggcgccgccg agctggtgcg ggcgggtttc gagacgctgg
     9961 tcgaggccgg ctatgccccg gagatcgcct acttcgagtg cctgcacgag ctgaagctca
    10021 tcgtggatct gatccaggag ggcggcctca gctacatgcg gttttcggtt tccgacacgg
    10081 ccgagtacgg cgactacacg cgcggaccga gagtggtgga cgcgcacgtt cgcggcgtga
    10141 tgaaggaact gctggccgag gtccagtctg gcaagttcgc aaaagaatgg attgacgaaa
    10201 acaagacagg ccgcaaaaag tttttggcga tgcgcgaagc atcccgccac caacccatcg
    10261 aagaagtcgg ccgggagcta cgcgcgatga tacccttcct caagaagagg aaggaagccg
    10321 gcgtaccgga agaccagcca gccacggctt ccgggcgcta gccgggaaag aacgcaatga
    10381 agatctccat ccttgacaca acactacggg acggcaccca aggggaggcc gtctcattta
    10441 ccgtcgaaga caaactgatg atcgcccaga agctggacga gctgggcatc gactacatcg
    10501 aaggcggttg gccgggctca aaccccaagg ataaagagtt cttctcacgc gcccgggaac
    10561 tccacctcaa gcatgcccgg ctgaccgcct tcggctcgac gcggttcgcc aagaacccgg
    10621 tggaccgcga ccccaatgtg cgcgccctgc tcgaggcagg cacgcccgtc atttcgattt
    10681 tcggcaagag ttgggatctg cacacgcaac gcgcgctcgg catcagtgaa gaagagaacc
    10741 tgaagctcat cgcggagacg gtgaagtatt tgaaggacca cggccgcgag gtgatctacg
    10801 acgcggagca cttcttcgac ggttacatct cgaacccgtc tttcgcgctg cggactctcg
    10861 aggccgcccg ggattcgggc gcggacattc tctgcctgtg cgacaccaac gggggcacgc
    10921 tgacgggccg gctgacggag atcacggccg acgtccggaa gcgtttcgat ggagtgatcg
    10981 gaattcacgc gcacaacgat acggatgtgg ccgtagccaa cgccgtggcc gcggtggaac
    11041 aaggcgcgac gcacgtgcag ggaacgatca acggctatgg cgagcggtgc gggaattcga
    11101 acctgtgctc catcatcgcc aacctggagc tgaagctggg gcacacgacc atcggcagcg
    11161 acaagctggt tcaactcact tcggtggcga agttcgtggc cgaactggcc aacctgaacc
    11221 tccggaacga ccagccgtac gtggggcgca gcgcgttcgc tcacaagggc gggatccacg
    11281 taagcgcggt gcttaaggac gcggcaacgt acgagcacgt tcccccggag acggtgggca
    11341 accggcagcg ggtgctgctg agcgacctgt cgggacggag caacatcctg tacaagctga
    11401 agcagcacgg tctggcggac cggctgacgg aggaggcgcg gcgggagctg ctcgagcgga
    11461 tcaagctcat ggagtaccag gggtatgaac tggaggcggc ggagggcacg ttcgagctgc
    11521 tggtgcgcga ggcgcttcac cccggaactc actttttcga tctggtgagc ctggatgtgt
    11581 cgacgcagat cgccggcacg aagggaacga gagtaacggc gacggtgacg ctgaaggcag
    11641 cggatggtgt gcactcggcg acggcgagcg ggcacgggcc tgtgaacgcg ctggatctgt
    11701 gcctgcggca gtgcctgtcg tcgctgtatc cgaccatcgc caacgtgcgg ctgacggatt
    11761 acaaggtccg cgtgctggaa ccgaagaagg ggacggcggc gcgggtgcgg gtgctgatcg
    11821 agtggtcgga tcaccgccgg agctggacga cggtgggggt atcggagaac gtgatcgagg
    11881 cgagctggca ggcgatggtg gacgcgctcc ggctggagct gatgcgcctg atggagcagg
    11941 acgagacgat cgagagggcg gtggaagatt actgctgggg agtgtgaggg aggccacagg
    12001 ctgaagcctg tgccaccgat ggcgtgagga acttctggaa acttcggcag ccggacgtgc
    12061 ggcgcatcgt tctggtggag agcgggccgc ggcacctgct ggaaggcgcg attcccgtct
    12121 tgcgccgact ctacggagaa tcgatcccaa tcgatctggt gacgtgtttc gccggcagcc
    12181 cggtggggtt cactgccggg aacggccgcg tgtatcaggt ggccgaccac cagggacggg
    12241 caggacggaa gcgcctctat cgggaactgt gcgcgctggc gccggacgcg ctggtgatgg
    12301 tctgcgcgga cgtgccgatc atgacgaagt ggaagtgggc gctggcgtgg aatctgccgg
    12361 ccaaagtcct gatcctcaat gaaaacggcg atttcttctg gttcgaccgc gcgcactggc
    12421 gggtgattct gcacttcatt gcgttccgct cggggctgac gggcggcggc atggtccgca
    12481 ccatcgccga cttcgcgctg tttcccttca cgctgacata cctgctgctc tacgccggtt
    12541 ggatccacgc ccggcggaag gtgcggctgg cgatgcggag tggggcaatg gggctggata
    12601 agacttaggg gcggatgccg actcgcctgg ttcaggccgc atccagcacg tcgatccgaa
    12661 g
//
LOCUS       DATIFQ010000154         3908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2733394, whole genome shotgun sequence.
ACCESSION   DATIFQ010000154 DATIFQ010000000
VERSION     DATIFQ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2733394"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VLE22_07245"
     CDS             complement(<1..1293)
                     /locus_tag="VLE22_07245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB14238.1"
                     /translation="MMWWDVIRSTILVYNGLVLVYFFAINTQYLVLMLVGFVRTWQAL
                     KAWHWGDLRWLTQSPLTPPISIIAPAYNEEANIVQSVRSLLMLHYPQFEVVVVNDGST
                     DGTLRLLIEEFVLRPVARCFDLTVPCRPIRAVYESREYPTLIVVDKENGGSKADAMNA
                     GLNVALYPLFCAIDADSVLEEEALLRVVRPFVDEPALTVACGGVIRIANGSEIRAGRM
                     VRVQLPRAPLPLVQIVEYLRAFFFGRMGWDAWNGLMIISGAFGVFDKQAVIRAGGYAP
                     DTHGEDIELVVRLHRQLRDEGKPYRIRFAPDAVCWTEAPTTLRALRNQRTRWQRGLID
                     TLWRHRAMLGRPRYGVIGLVSMPCFLLFEMLGPLVELTGYVLVPVCFLLGLVDPRFFA
                     AFLGVMILYSALTGMTAVLLEDVTFRRHPHLWHLIVLTV"
     gene            complement(1290..2363)
                     /locus_tag="VLE22_07250"
     CDS             complement(1290..2363)
                     /locus_tag="VLE22_07250"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB14239.1"
                     /translation="MEIHLHDPLSLLLIFLLALLVAVLALFALLLIQRMAGMIWTQHA
                     QRREASLTPLLLAALTDPSGVQTLVGAVKGMDRLIVRDMILRLALDLKGEEVETLAGL
                     YRDLGLLRRDLDALRARSWMRRSAAAARLGTLRSLTVLPLVRTALSDPVVRVRLAVVR
                     AVGETGDREALTALVPLLGDSNPGVVRAAAEVLTLRGREVVEDVLAFLRSAAAASARA
                     AAVDVLGFMRAPQATDALLALVEDADPALRVKVVKALAAIGDPRLRSLLENLLEDPQW
                     EIRCQAAKGLSLLGSSDSVACLRRALEDPHWWVRFHAAVALAELRPAGRNALQEEVVE
                     GGPLSRMIARYVLDRLGASRVVS"
     gene            complement(2363..3727)
                     /locus_tag="VLE22_07255"
     CDS             complement(2363..3727)
                     /locus_tag="VLE22_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14240.1"
                     /translation="MSDKTSSRHEPWLLGGVAFILLWLPLTVAWPGLLGAQPADPLVE
                     AQAHLRAGKAEEALIIYDHMVEAQPQNLEAHKGRANALGRLGRYADALEEYARVVASK
                     PEDVEAHIGRARILGWMGRYSEAEAECRRVLTDNPNVVEAYLQLGTILGWQRKYSEAA
                     ELFERARQLAPHDPESFVGLARLRFWQDDLEGAKTFYDAALRLDPSSVDAREGLDRIA
                     KIPRPWRFRTYVGFRFDALNGGLSDWYQETIAVSARPWKETTFLLNVDQYRRFDQDAT
                     QLTIGAAHELPAGFTLGGSFTYGFDADIVARKIYVVDLGYRLASTITPLIRYRHASFG
                     AGVREDVVSPGVEVTWSPYVAVLGRYYYADVSTTGTNHAGFGRVTLFPEGSVSAYGSF
                     GYGGDVLNANQVALREITVTTAGAGVTWRLTDRVALRLDYEYEDRRTSYIRNGVNFGI
                     SMDF"
BASE COUNT          875 a         1218 c         1129 g          686 t
ORIGIN      
        1 cacggtcaag acaatgaggt gccagagatg cggatgccgt cggaaggtga catcttccaa
       61 aagcaccgcg gtcataccgg tcagtgcgct gtaaaggatc atgacgccga gaaaggccgc
      121 gaagaaacgt gggtccacca gtccgagaag gaagcacacg ggtaccagga cgtatcccgt
      181 caattcgacg aggggcccga gcatctcaaa caggagaaag cacggcatcg aaaccagccc
      241 gataaccccg taccgtggcc gtcccagcat ggcccggtga cgccacagcg tatcaatgag
      301 gcctcgctgc caccgcgtac gctgatttcg gagcgcgcgc agtgtcgtcg gtgcttccgt
      361 ccaacagact gcatccggag cgaaacgaat ccggtagggt ttcccttcgt ctcgcaactg
      421 cctgtgtagt cgcaccacca gctcgatgtc ctcgccgtgt gtatctggag cataacctcc
      481 cgcccggatg accgcctgtt tatcgaacac accgaaggct cccgatatga tcataagccc
      541 gttccatgca tcccagccca tgcggccgaa gaagaatgcg cgcagatact caacgatctg
      601 aacgagcggg aggggcgccc ggggaagctg gacacgcacc atacgccccg cacggatctc
      661 gctaccgttg gcaattcgaa tgacgccccc acaagcgacg gtcagcgcag gctcgtcgac
      721 aaaaggacgg accactcgca acagcgcttc ctcctccaag actgagtccg cgtcaatagc
      781 acaaaagagc gggtacaagg ccacgttgag ccccgcgttc attgcgtccg ccttggagcc
      841 tccgttctct ttgtcaacga ctatcagcgt gggatactcc cgcgactcat agacggcccg
      901 aatgggccga cagggcactg tcaggtcaaa gcaacgggcc acgggccgca ggacgaactc
      961 ttcaatcagg agacgaaggg taccgtcggt agagccgtca ttaaccacca cgacctcgaa
     1021 ctggggatag tgaagcatca agagggaacg aacgctttgg acgatattgg cctcttcatt
     1081 gtaggctggc gcgatgatag agatcggcgg cgtcagggga gattgcgtca accaccgaag
     1141 atctccccag tgccacgcct tgagtgcttg ccacgtcctg acgaacccta ccagcatcag
     1201 gacaaggtat tgggtgttga tggcgaagaa atacaccaag accaaaccgt tataaaccaa
     1261 aatcgttgag cgaatgacgt cccaccacat catgaaacca cccgagacgc cccgaggcga
     1321 tcgaggacat atcgagcgat catacgcgag agaggtccgc cctccaccac ttcttcttgt
     1381 agggcattac gaccagcggg gcgcaactcc gccagcgcca cagcagcatg gaagcgcacc
     1441 caccagtgcg gatcctcgag agcacgacgc aggcaagcca ccgaatccga gctgcccagg
     1501 aggctcaatc ctttggctgc ttggcaacga atctcccact gtggatcttc caacaggttt
     1561 tccagcaggc tcctcaagcg cggatctccg atggctgcca aggctttcac cactttgact
     1621 cgcagagccg gatcagcatc ctcgaccagg gccaacaggg cgtctgtcgc ctgcggtgcg
     1681 cgcatgaagc ccagtacatc gaccgcagcc gcgcgagccg aagcggcggc tgcactccgg
     1741 agaaacgcca agacgtcttc gacgacctcg cgcccccgca gggtgagcac ctctgcggcc
     1801 gcccgcacca cgcccggatt ggagtccccg aggaggggca ccaacgcggt taaagcctct
     1861 cgatctcccg tttcccccac agcgcgcacc acggcgaggc gtacacggac gacagggtcg
     1921 gacagtgcag ttcgaaccaa gggtaggacg gtgaggcttc gtagcgtgcc tagtcgcgcg
     1981 gcggcggcgg accgtcgcat ccagctccgc gcccgaagtg cgtcaagatc gcgcctcaat
     2041 agacccaggt cccgataaag tcccgctagg gtttcgacct cttcaccctt gaggtcgagc
     2101 gctaggcgta gaatcatgtc gcgcacaatg agacgatcca tccccttgac ggctcccaca
     2161 agcgtctgta cgccactcgg gtccgtgagg gcagcgagta ggagcggcgt gagagaggct
     2221 tctcgcctct gggcgtgctg cgtccatatc atcccagcca tgcgctgtat cagcagcaaa
     2281 gcaaacagcg caaggaccgc cacgagcaat gcgagcagga atatcaggag cagcgacaat
     2341 gggtcgtgta ggtggatttc catcaaaaat ccatcgagat tccgaagttc acaccattgc
     2401 gaatgtacga cgtgcgacga tcttcgtact cgtaatcgag gcggagtgcg acccggtcgg
     2461 tcaggcgcca cgtgacccca gcgcccgccg tggtcacagt gatctcgcgt agtgccacct
     2521 ggttcgcgtt aagaacatca ccaccgtagc cgaagctacc ataggcactt acggaacctt
     2581 cgggaaagag ggtcacccga ccgaagccag cgtggttggt accggttgtg gaaacgtcgg
     2641 cgtaatagta tcggccgagc accgcgacgt agggagacca ggttacctcc acgccgggcg
     2701 agacgacatc ctcccgaacg ccagcgccaa agctggcgtg tcgataacga atcaggggcg
     2761 tgatcgtcga agcaaggcgg tagcccaagt cgacaacata gattttccgg gcaacaatat
     2821 ccgcatcgaa cccatatgtg aagctgccac caagggtgaa acctgcagga agctcgtggg
     2881 cggccccgat cgttagttga gtagcgtcct ggtcaaaccg acgatactga tccacgttca
     2941 acagaaaggt cgtttctttc catggtcgtg cgctcacggc gattgtctcc tgataccaat
     3001 cactgagtcc cccgttgagc gcatcgaacc ggaaccccac gtaggtgcgg aagcgccagg
     3061 ggcgcgggat cttcgctatt cggtccaaac cttcccttgc atccacactg cttggatcca
     3121 agcggagcgc ggcatcgtaa aaggttttcg ctccctcgag atcgtcctgc caaaaccgaa
     3181 ggcgcgcaag tccgacgaat gattctgggt cgtgtggggc aagttggcgt gcacgctcaa
     3241 aaagctcggc ggcctccgag tatttgcgtt gccatcccaa gatcgtgcca agctgcagat
     3301 aggcctcgac gacattggga ttatccgtga gtactcgacg gcattccgcc tcggcctcgg
     3361 agtaccggcc catccatccc agtattcggg cacgcccgat atgcgcttct acgtcttccg
     3421 gcttggaggc tacaacccga gcgtattcct caagcgcgtc cgcgtatcgt ccgagtcggc
     3481 caagagcgtt ggcacgtcct ttatgtgctt cgaggttttg aggctgggcc tccaccatat
     3541 gatcgtagat tattaaagcc tcctcggcct ttcctgcgcg aaggtgtgcc tgcgcctcca
     3601 ccagcgggtc ggccggctgg gccccaagaa gtcccggcca tgcaacggtc aagggcagcc
     3661 aaaggaggat gaaagctacg cctcctagca gccagggttc atgcctgcta ctggttttgt
     3721 cactcatgag agcgcagggc tcaggcggtt cggtgccagc cgtcgtccga gcgacggcag
     3781 cgagaatctg cgtcgcccgg ggacccgccg cggagaacga tttcggtcca atcatcgctt
     3841 tggcgactgg ggatacgaca tgggacccct acgctttgag gacaagagtc catggccccg
     3901 gatactca
//
LOCUS       DATIFQ010000155         3652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1201325, whole genome shotgun sequence.
ACCESSION   DATIFQ010000155 DATIFQ010000000
VERSION     DATIFQ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201325"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..805
                     /locus_tag="VLE22_07260"
     CDS             416..805
                     /locus_tag="VLE22_07260"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB14241.1"
                     /translation="MLGILEKFSATSRGAPDRRANDRFPMVRELHYKVMHSKGRDEIG
                     TGQTVNISSAGVLFTAQSPLPPGRRLEVSISWPAQLDGKCGLKLIARCRVVRCTDTTV
                     ALEIDRYEFRTAGREFAAATVNQVAPL"
     gene            1145..2446
                     /locus_tag="VLE22_07265"
     CDS             1145..2446
                     /locus_tag="VLE22_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14242.1"
                     /translation="MKHENRRAFLQTAGAVVLARQAFPVLGANDRINLGVVGIGGRGR
                     DHIDEFVKLPQCRIVAVCDVDQAGRERGVAQVERLAGSKPKQYADMRQLFEDKEIDAV
                     SFATPNHWHALGTIWACQAGKDVYVEKPPSHNIYEGRKMIEAARKYNRMVQVGSQSRS
                     MGYVQRAVRLLGEGVIGKVYEAKGLCFNPRKSIGHTPDEPVPPGINWDLFLGPAPMRP
                     FSRNRFHYNWHWFWDTGNGDIGNQGIHEMDKARWGLNRGLPLHVVSTGGKFVYQDDQE
                     TPNTQIAVFDYGDAQVTFDVRGLITPGEGDVRREDHFVGNVFFGSEGYLTLDGNGFRV
                     YKGYERKLVMDEKPGPEGDTGPHMENFLAACRSRRYQDLTADIEIGVTSVALVHLANI
                     SYRVGRRLTIDPATWRIKSDPEAERMLTRPYRAPYVVPDKV"
     gene            complement(2486..2653)
                     /locus_tag="VLE22_07270"
     CDS             complement(2486..2653)
                     /locus_tag="VLE22_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14243.1"
                     /translation="MPLNLRKPDRMRLALLALMLVVAYGGHTQSWAFSGKDMGVPPCL
                     PVSAIVSEWRG"
     gene            complement(2690..3475)
                     /locus_tag="VLE22_07275"
     CDS             complement(2690..3475)
                     /locus_tag="VLE22_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HSB14244.1"
                     /translation="MKRRRILMLAAILMLLLAGIVVFGIWVAKGGLEKELAPAFSEPQ
                     AAWRETHGPLIHTRWKQDGPYAALAPGRADLGCWSVAFAQVLAFHRLEPRGRVHYQTR
                     RGMTIDETLDGSVDWDRMVPAIAPDTPPENSLATARYCFQAAAVVQKDFGSGEYMDVA
                     ITPREIAEHYGCTVARIDSNVAETIRSELREGHPLVAYFDDILGIKIVRNGHAAVIDG
                     MAGDNDRMFVHVNLGWGGASDGWYDFEKLTKERKLLYVFRIAP"
BASE COUNT          732 a         1133 c         1136 g          651 t
ORIGIN      
        1 cgcatacgcg cggcttctac ggccgcgccg ccgtgctcta tcaccacgcg cggggaacgc
       61 cgccgaaggg gctcttctgg ctgccgatgc gggatggccg ctggcggatc gactggccgc
      121 gcacgaatcg tggcgcgtaa gacccgcgtc gcagtaatcc ctccactttg tctgcccgga
      181 cggaaaaacg gggactgact tggactgact ccagtttcgc tgcagttgcg aaagtggtgt
      241 ctgtccccgc ttttcccgaa tggacgcacc tcaggggcat aggctagcac tggcgcaaca
      301 ccgggggagt acagtagcac catcgggagc accgtcacaa atgaacccat ctacctattg
      361 ccgatgcggg cgggaaagtg gaggatcaat aagaaacaag aaagaggatc tgcggatgtt
      421 gggaatattg gagaagtttt cagctacatc gaggggcgcc ccggatcgga gggcgaacga
      481 ccgttttccg atggtgcgtg aactccacta caaagtgatg cattcgaagg gcagggacga
      541 aatcgggacc ggccaaacgg tcaacatcag cagtgctgga gttttgttca cggcacagtc
      601 gcctctgccg ccaggcaggc ggctggaggt ctccattagt tggccggccc aattggatgg
      661 caagtgcggt ctgaaactaa tcgcccgttg tagggtcgtg cgctgcacgg acacaaccgt
      721 cgccctggag atcgacaggt acgagttcag gaccgctggc cgggagtttg cagccgcaac
      781 cgtgaatcag gtcgccccgc tctagggcgg agacatcgcc ttcgacgtcc ggattgaaga
      841 gcgtacaccg catcgtaccg gtacttaaca ccggcgtcta gtcctagcat tcagcaaagc
      901 aagggcccac aatactcctg gtgcctcgaa tgcacactgt ggattcgatg aggcccgcgt
      961 ctcccgcacg cccaatccgg cggacgtgag gggtccctac caagccgggc tcaacctgag
     1021 cggcggcgca ctgccatgcg ccgccatatt ctttccatga ggcagggtgc gcagccaccc
     1081 gcagcccggc gccgccttcg ttggagtaga attgctttgc catcgcatcc aaagagcaat
     1141 caccatgaaa cacgagaatc gacgggcatt tctccaaacg gccggcgcag tggtcctggc
     1201 gcggcaggcc ttcccggtgc tgggggcgaa cgaccggata aacctcggag tggtgggcat
     1261 cggcggccgc ggccgcgatc acattgacga gtttgtgaag ctgccccagt gccgcatcgt
     1321 cgccgtctgc gacgtggacc aggccggccg cgagcgcggc gtcgcccagg tggaacggct
     1381 ggcaggctcg aagccaaagc agtacgccga catgcggcaa ctgttcgagg acaaggagat
     1441 cgacgctgtc tcgttcgcca cgccgaatca ctggcacgcg ctcggcacaa tctgggcctg
     1501 ccaggccggc aaggatgtgt acgtcgagaa gccgccgagc cacaacatct acgagggccg
     1561 caagatgatc gaggccgccc ggaagtacaa ccggatggtg caggtcggct cgcaaagccg
     1621 cagcatgggc tacgttcagc gcgccgtgcg gctgctcgga gagggcgtga tcggcaaggt
     1681 ttacgaggcg aagggattgt gcttcaaccc gaggaaatca atcggccaca cgcccgatga
     1741 gccggtgcct cccggcatca actgggacct gttcctcggc cccgcgccca tgcgtccctt
     1801 cagccggaac cgtttccact acaactggca ctggttctgg gacaccggaa acggcgacat
     1861 cggcaaccag ggcatccacg aaatggataa ggcgcggtgg gggctcaacc ggggcctgcc
     1921 gctgcatgtg gtctcgaccg gcggaaagtt cgtctaccag gacgatcagg agacgcccaa
     1981 cacccagatc gccgttttcg attacggcga cgcccaagtc acctttgacg tccgcggcct
     2041 gatcactccg ggagaaggcg atgtccgccg ggaggaccat ttcgtcggga acgtcttttt
     2101 cggcagcgag ggctacctca cgctcgacgg caacggtttc cgcgtctaca agggctatga
     2161 acgcaagctg gtaatggacg aaaagcccgg tcccgagggc gacaccggcc cccacatgga
     2221 gaacttcctg gcggcctgcc gcagccgcag ataccaggac ctgaccgccg atatcgagat
     2281 cggggtcacc tccgtcgccc ttgttcattt ggccaacatc agctaccgcg tcgggcggcg
     2341 gctgaccatt gacccggcca cttggcggat caagagcgat ccggaagcgg agcggatgct
     2401 cacgcgtccc taccgggccc cttacgtcgt tcccgacaaa gtctgaggtt cttgtggggc
     2461 gggccccctg gcccgcgcgg ggctctcagc cccgccattc actgacaatc gccgagacag
     2521 gcaagcaggg aggaactccc atgtccttgc cggagaacgc ccagctttgg gtgtgcccgc
     2581 cgtaagcgac gactagcatg agtgcgagga gggcgaggcg catgcggtcg ggtttgcgga
     2641 ggttgagggg catgggttca gatctgtcgc atcgaggtgc tgttgatcct tacggtgcga
     2701 tacggaatac gtaaaggagc tttcgttcct tggtcagttt ttcaaaatca taccagccgt
     2761 cgcttgcgcc gccccagcca agattcacat gaacgaacat tcgatcgttg tccccggcca
     2821 tgccgtcaat aaccgcggcg tgtccgttgc gcacgatctt tatgccaaga atgtcgtcga
     2881 aatacgcgac aagggggtgg ccttcccgta gctccgatcg tatggtttct gccacgttgg
     2941 agtcgatccg cgccaccgtg cagccatagt gttcggcgat ctcgcgcgga gtgatggcga
     3001 cgtccatgta ttctccgctg ccgaagtcct tttgcacgac cgccgccgct tgaaaacagt
     3061 aacgcgccgt tgcgagtgaa ttctccggcg gcgtatccgg cgctatggcc ggcaccatgc
     3121 gatcccaatc gaccgaacca tcgagcgtct catcaatcgt catgccccgg cgagtttggt
     3181 agtgtacccg ccctcttggc tccagacggt ggaaagcgag tacttgggca aacgcgaccg
     3241 accagcaccc gagatctgcc cggcccggcg caagcgcggc gtacgggccg tcttgtttcc
     3301 aacgcgtgtg gatcagcggc ccgtgcgtct cccgccacgc cgcctgtggt tcggaaaagg
     3361 cgggcgccaa ttctttttcg agcccgccct tcgcgaccca gatgccgaaa accacgatgc
     3421 cggccagaag aagcatcaga attgccgcca gcattaggat gcgccttctt ttcataagtg
     3481 gtcatccact acgacaccaa aatcctcctt gaccgggtgg tggagagtcc ccaatgaaaa
     3541 ccacgcgctg ctcccccgga gcgggcgagg cggcggaaac cacgagcacg gcggccctcg
     3601 caggtttccg atcggggaaa aacggggact gactccagtt tcgctgcagt tg
//
LOCUS       DATIFQ010000156         3026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2542417, whole genome shotgun sequence.
ACCESSION   DATIFQ010000156 DATIFQ010000000
VERSION     DATIFQ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542417"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1016)
                     /locus_tag="VLE22_07280"
     CDS             complement(147..1016)
                     /locus_tag="VLE22_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB14245.1"
                     /translation="MPAAGTRKKLKRNNYVLVIFDSCRYDSWVKARPRVMRKLGTVEK
                     RWSYASWTSPSHYNFLIGLMPHRSPKQVFASEYYQKEFVQFNERLGCRDFEFKSLIPR
                     LYLPDFLRETLGYRTHAMVSLPVLNPRTVLNQGFDTYSLMPKHNDMRAMVRQMRFSKE
                     RPSFYLLNVGETHYPYALPDEPPDEWPRISGVHGVFKHLDDHIVGGKLVSRKEKFFDQ
                     AKLDMLRKRQVEAVRYLDNVVEELFDTVPKNTYITITADHGELFGEEGYFGHGPIQHE
                     KVFEVPFVEGKIR"
     gene            1133..1600
                     /locus_tag="VLE22_07285"
     CDS             1133..1600
                     /locus_tag="VLE22_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14246.1"
                     /translation="MPFCCQCGSATTDTDTYCGACGTRQPVTPKRKQAANGLSPRTAS
                     ILCYIPVVGWIPAIVVLASQRFRNAREVRFHAFQGIYLFVAWLLVDWVIAPLYHLPLW
                     HGGHLFPGIFVGGILKTVIFFGWIFMLIKTSQEETFKLPLLGELAERSVAEQR"
     gene            1652..2674
                     /gene="argC"
                     /locus_tag="VLE22_07290"
     CDS             1652..2674
                     /gene="argC"
                     /locus_tag="VLE22_07290"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HSB14247.1"
                     /translation="MNNYSTPVRAGVVGFRGYSGAELLRILDRHPSAEPWLLEHRADS
                     EERHHPRGDRSHPRIPCTPDAVQSEGIGVVFLATPPEVSMDLTPAMLEAGAKVIDLSG
                     AFRLRTVENYARWYKETHTQPSLLAEAVYGLPEFCRDAVRGARLVSNPGCYPTAANLA
                     IRPLIEAGIVDRRAGIVCDAKSGVSGAGRKPSLKTSFCEVVENFSAYSILKHRHVAEV
                     LLTSGLEEPEFSFTAHLLPLDRGILETVYVRLLPGRDADSVLSAYEKRYSGEPFVRLY
                     DPGHVPDLRAIQHTNFCDIGFQVDAASGRAVIVSVIDNLVKGAAGQAVQNMNLLLGYP
                     ETEGLL"
     gene            2671..>3026
                     /locus_tag="VLE22_07295"
     CDS             2671..>3026
                     /locus_tag="VLE22_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14248.1"
                     /translation="MKLLVKLGGTLLDVEESRLRIARELAAVAAGGHQLVVVHGGGKQ
                     MTRFLTERGIESRFIDGLRVTTPETIDAVLKVFAGSVNKQLVASLIQAGARAVGLSGI
                     DAGLVEAEPLREELGAV"
BASE COUNT          556 a          979 c          890 g          601 t
ORIGIN      
        1 ggcatactga acatccccat ggacgtgcgc gtctccaccc atccacaccg ccagcgccgc
       61 cactcccagc agcacaagga accgcgaccg ttccgagccg cgaccatagg gagcggtcat
      121 ttcttcccca cccctctcac gcaccttcac cggatcttgc cctccacgaa aggcacctcg
      181 aagaccttct cgtgctggat cggtccgtga ccgaaatacc cctcttcccc gaacagttcg
      241 ccgtgatcgg ccgtaatcgt gatgtaggtg tttttcggaa cggtatcgaa caattcctcg
      301 accacattgt cgaggtaccg gaccgcctcg acctgccgct tgcggagcat gtccagtttc
      361 gcctggtcga agaacttctc tttcctggaa acgagcttcc cgccgacgat atggtcgtcc
      421 aggtgtttga aaacgccatg gacgccgctg atccgtggcc actcatccgg aggttcgtcc
      481 ggaagcgcgt agggatagtg cgtttcaccg acattcagta ggtagaacga gggccgctcc
      541 ttggagaagc gcatctgtcg gaccatggcc cgcatgtcgt tgtgcttcgg catcagcgag
      601 taggtgtcga acccctggtt cagcaccgtt cgggggttca gcaccggcag ggaaaccatc
      661 gcgtgcgtcc ggtagccgag agtctcccgc agaaagtccg gcaggtaaag ccgggggatc
      721 aatgacttga attcgaaatc ccggcagccc agacgctcat tgaactggac gaactccttc
      781 tggtagtact cggacgcaaa cacttgtttc gggctgcgat gcggcatcag gccgatcagg
      841 aaattgtagt gggagggcga cgtccaggac gcgtacgacc agcgcttctc gaccgtgccg
      901 agcttcctca tgacccgcgg acgcgccttg acccacgaat cgtagcggca actgtcgaag
      961 attaccagga cgtaattgtt tcgtttaagc tttttgcggg ttccggcggc tggcactatg
     1021 tagtcattat atcaagccgc ccgaacgccc ccttcttctc ccggcacagg cgaaacaaag
     1081 ttgcaggaac aaccaagtcc cgcgtaacgt aagagttatc ggggaaagag aaatgccttt
     1141 ctgttgccaa tgtggaagcg ccaccaccga taccgacacc tactgcggcg cctgcggaac
     1201 acgccagccg gttacaccga agcgtaagca ggctgccaac ggcctcagtc ctcgcacggc
     1261 atccattctc tgctacatcc cggtcgtggg ctggataccc gcgattgtcg tcctggcctc
     1321 ccagcggttc cggaacgcgc gtgaggtccg ttttcacgcc tttcagggca tctacctctt
     1381 tgtcgcctgg ctgctggtgg actgggtgat cgctccgctc tatcaccttc cgttgtggca
     1441 cggaggccac ctgttcccgg gcatcttcgt cggcggcatt ctcaaaactg tcatcttctt
     1501 cggctggatc ttcatgctga tcaagacgag ccaggaagag accttcaaac tccctctgct
     1561 cggcgaactc gccgagcgtt ccgtcgccga gcaacgttga gcgcgccgtc cctccggccc
     1621 cgcccgcggt gtggtatgtt tattcatacg gatgaataat tactcaacgc ccgttcgcgc
     1681 cggcgtggtc ggtttccgcg gttacagcgg cgcggaactg ctgcgtattc ttgaccgtca
     1741 ccccagcgcc gagccttggc ttctcgagca ccgcgccgac tccgaagagc ggcatcaccc
     1801 gcgcggagat cggagccacc ctcgaatccc ctgcactccc gacgcggtcc aatccgaggg
     1861 aatcggtgtc gtctttctgg cgacgccgcc cgaggtctcc atggatctca ccccggcgat
     1921 gctggaggcg ggggccaagg tgatcgatct cagcggcgcg ttccgcttga ggacggtgga
     1981 gaactacgcg cggtggtaca aggagacgca cacccagccc tcgttgctcg ccgaagccgt
     2041 ttacggactt cccgagtttt gtcgcgacgc cgtgcgtggc gcccggctcg tctcaaatcc
     2101 gggctgctat ccgaccgcgg cgaatcttgc catccggccg ctcatcgaag ccggcatcgt
     2161 ggaccggaga gcgggtatcg tctgcgacgc gaagtccggt gtgagcggcg ctgggcgcaa
     2221 gccgagtctg aaaaccagct tctgcgaagt ggtggagaac ttctccgcct actcgattct
     2281 gaaacaccgg cacgtggcgg aggtcttgtt gacctccggg ctcgaagagc ctgagttcag
     2341 cttcaccgcg catcttctgc cgctcgaccg cggcatcctc gaaaccgtct acgttcgcct
     2401 cttgcccggc cgggatgcgg acagcgtgtt gtcggcgtac gaaaagcgct attccggcga
     2461 gccgttcgtc cggctgtacg accccgggca cgtcccggat cttcgggcca tccagcacac
     2521 gaacttctgc gacatcggct tccaggtgga tgcggcgagc ggccgcgcgg tcatcgtcag
     2581 cgtcatcgac aatctcgtga agggcgcagc cggccaggcc gtccagaaca tgaatctgct
     2641 tcttggttac cccgagaccg aggggcttct gtgaaactgc ttgtcaaact cggcggcacg
     2701 ctgctcgacg tcgaggaatc acggctgcgg atcgcccgtg aactggctgc cgtcgccgcc
     2761 ggcggccatc aactcgtcgt cgtccacggc ggcgggaagc agatgacgcg cttcctgacc
     2821 gaacgcggca tcgaaagccg ctttatcgac gggctgcgag tgacgacgcc cgaaaccatt
     2881 gacgccgtct tgaaagtttt cgcgggcagc gtcaacaaac aactggtggc atcgctcatc
     2941 caggccgggg cgcgggccgt cggtctttcg ggcatcgacg ccggcctcgt cgaggccgaa
     3001 ccattgcgcg aggaactcgg tgcggt
//
LOCUS       DATIFQ010000157         2814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_817825, whole genome shotgun sequence.
ACCESSION   DATIFQ010000157 DATIFQ010000000
VERSION     DATIFQ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817825"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1406
                     /locus_tag="VLE22_07300"
     CDS             258..1406
                     /locus_tag="VLE22_07300"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HSB14249.1"
                     /translation="MPFPRRRSPLQFSGQDLLRLESISKSRTEEKRRSLRAAFLLDSR
                     SGQSDAAIARHHHVSRSTVVLCLQKFLQFGLDAALGELPRPGKPRQLPDDAIAWVQDC
                     ACRKPKELGYSYELWTYRLLLAHIRQHCAAVGHPALLKLSRSKLHKILRQGELRPHKI
                     RYYVEKRDPDFAVKMANVLHVYKEVEIVNEYRRGETGRQLRMVTISYDEKPGIQALAT
                     TTPDRPPVVGTHPSHLRDYEYVRLGTVSLLAGLDLHSGRVTETVSDTHNSSDFIAFLR
                     KLDGAYPEHHQIRLVLDNHSAHTSKETRGYLQTMPHRFVFVFTPTHGSWLNLIENQFS
                     KMTRTMLRGIRVTSKQELIDRIHQYFEEINAAPVVFRWKYKMDETIIV"
     gene            complement(1445..2569)
                     /locus_tag="VLE22_07305"
     CDS             complement(1445..2569)
                     /locus_tag="VLE22_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011169510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HSB14250.1"
                     /translation="MPRAVDPLVLSQDQRSELQGWVVALGTPQQVALRCRIILAVAAG
                     RTETMIAAENGVNRKTVRLWRERFRDGGPQALWEIAPGRGRKPSYDVTRIKGVIDATL
                     QTKPKGSTHWSCRTMAAAQGISKSSVSRIWRSHNLKPHRTETFKLSRDPKFLEKLTDV
                     IGLYLNPPDQAIVLCVDEKSQIQALQRTQPGLPLKKGRCGTITHDYKRHGTTTLFAAL
                     DVLAGKVIGDCYKRHRHQEFLRFLRRINREFPGKRSLHLVMDNYGTHGTPEVKDWLDQ
                     HPRFKVHYIPTSSSWLNLIERWFGELTSKRIRRDSFLSVEDLITAINEFLAAWNEKPK
                     PFVWTATVESIVNKLARCRQTLEQIQPGCTARKTRKRKAS"
     gene            2568..>2814
                     /locus_tag="VLE22_07310"
     CDS             2568..>2814
                     /locus_tag="VLE22_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HSB14251.1"
                     /translation="MTYYVPRANVLSSYLWDTTLADRGDAAACVKAGYRYLHGVAAKR
                     DERKAAEYFRKAGPRVPSAVAWLCFMEVACRKKPFPAS"
BASE COUNT          574 a          823 c          826 g          591 t
ORIGIN      
        1 cgccacctga aacatcccgg ctttatccgt gtagaacgcc agcgggcggc cgaactgctc
       61 cagatatgcc cccagcagac gcctgttctc ttcgctggtg tcgtgccgca cgaagcgggc
      121 gaatagccgg ctggtggcat cgtcaatcat cgaaatcaga tagatcgttt cgccgcgccc
      181 ttccagccag tcgtgagtgc tagatgatcg ttctctaaat accgatacat ttgacccgat
      241 ttatgtttta atgggtcatg ccctttccaa ggaggcgcag ccccctccag ttcagtgggc
      301 aggatttgct caggctggag tccattagca agtcgcgaac cgaggagaag cggcgcagtc
      361 tccgggcagc gtttcttctg gactcgcgat ctggccagtc cgatgcggcc attgcgcgac
      421 accaccatgt cagccgcagc acggtggtgc tgtgccttca gaagtttctc caattcggac
      481 tggacgcggc gctgggggaa ttgccccgac ccggcaagcc acgccaactg ccagacgacg
      541 cgattgcctg ggtgcaggat tgtgcttgcc ggaagccaaa ggaactgggc tattcctacg
      601 aattgtggac gtaccggctg ctgctcgctc acatccgcca acactgcgcc gccgtcgggc
      661 atccggcgct actcaaactg agccgctcca aactccacaa gatcctgcgg cagggcgaac
      721 tccggcccca caagattcga tactacgtgg agaagaggga tccggacttc gcggtcaaaa
      781 tggccaacgt gctgcatgtc tacaaggagg tcgagatcgt caacgaatac cggcggggcg
      841 agaccggccg gcaactgcgc atggtgacga tctcgtatga cgagaagccc ggcattcagg
      901 ccttggccac gacgacgcct gatcggccac cggtggtagg gacgcatccc agtcacctcc
      961 gggattacga atacgtgcga ctggggacgg tgtcgctact ggccggactg gatttgcaca
     1021 gcggcagagt gaccgaaacc gtcagcgata cgcataacag cagcgatttc atcgccttct
     1081 taaggaagtt ggacggcgcc tacccggagc accaccaaat tcgattggtg ctggacaacc
     1141 attccgcaca cacctccaaa gaaacgcgcg gttacctgca aactatgccc caccgcttcg
     1201 tttttgtgtt caccccgacc cacggctcgt ggctgaacct gatcgagaat caattcagca
     1261 agatgacgcg tacgatgctg cgaggaatcc gcgtgaccag caagcaagaa ctgatcgacc
     1321 gaattcatca atacttcgag gagatcaacg cagccccggt tgtctttcgt tggaagtaca
     1381 agatggatga gaccatcatt gtatagctaa taagagaacg ctaatctagt gtagtgtccc
     1441 cgaattagct ggcctttcgt ttgcgggtct ttcgggccgt gcagccgggc tggatctgtt
     1501 caagagtctg gcggcagcgc gcgagtttgt tcacgatgga ttcgacggtc gccgtccaga
     1561 caaacggttt gggtttttcg ttccaggcgg cgagaaactc gttaatggcc gtgatcaagt
     1621 cctcgacgct taagaaggaa tcgcggcgga ttctcttgct ggttagctcg ccgaaccagc
     1681 gctcaatcaa gttcaaccaa ctggagctgg ttggaatgta gtgcaccttg aaccgaggat
     1741 gttggtcgag ccagtccttc acctccggcg tgccatgggt gccgtaattg tccatcacca
     1801 ggtgcagcga cctcttgccg gggaactcgc ggttgatgcg gcgcaggaac ctcagaaact
     1861 cctggtggcg atgacgctta taacagtcgc cgatgacttt gccggcaagc acgtccagcg
     1921 ccgcgaacag agtcgtcgtt ccgtgtcgtt tgtagtcgtg ggtgatcgtg ccgcagcgcc
     1981 ctttcttcag cggcagcccg ggctgagttc gttgcagagc ttgaatttgg ctcttctcgt
     2041 ccacacaaag cacaatcgcc tggtcgggag gattcagata aagaccgatc acgtccgtga
     2101 gtttctcgag gaatttcgga tcgcgtgaca gcttgaacgt ctcggtgcga tgcggcttga
     2161 ggttgtgact gcgccagatc ctgctcacgc tggacttgct gatcccttgt gccgccgcca
     2221 ttgtgcggca actccaatgc gtacttcctt tgggcttggt ttgcagagtg gcgtcaatca
     2281 cgcccttgat tcgagtcaca tcgtaactgg gcttccggcc ccgaccggga gcgatttccc
     2341 aaagcgcttg aggcccgccg tcgcggaaac gctcgcgcca gaggcgaacc gtcttacggt
     2401 tcacgccgtt ctccgcggcg atcatcgtct ccgtcctgcc agcggcaacc gccaagatga
     2461 tgcggcaacg taacgcaacc tgctgtggag ttcccagggc aaccacccag ccttggagtt
     2521 cgctgcgctg gtcctgtgac aggacgagag gatcaaccgc gcggggcatg acctattatg
     2581 tcccgcgcgc caatgtgttg tccagctatt tgtgggacac tacactagct gatcggggtg
     2641 acgcagcggc ctgcgtgaag gcgggatacc ggtatttgca cggcgtggcc gcaaagcggg
     2701 acgagcgtaa ggctgccgaa tacttccgaa aagccggccc cagggttcca tctgccgtgg
     2761 cttggctgtg cttcatggaa gtggcctgcc ggaagaagcc gttccctgcc agcg
//
LOCUS       DATIFQ010000158        11144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2350761, whole genome shotgun sequence.
ACCESSION   DATIFQ010000158 DATIFQ010000000
VERSION     DATIFQ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11144
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350761"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VLE22_07315"
     CDS             <1..266
                     /locus_tag="VLE22_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14252.1"
                     /translation="ISKFNTYILESDSVTGPWRLVTYMKDFGQQAYFVNIPSKFINPD
                     GRTFWLMYAANFSNGNKNWHTNHQPDPPGSRYGMCLQEVRLGA"
     gene            439..3540
                     /locus_tag="VLE22_07320"
     CDS             439..3540
                     /locus_tag="VLE22_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HSB14253.1"
                     /translation="MSVNIGLDIGAISLKLAALGKPEDRPILASLCADKPAFRLIDWL
                     APDGRRLPLVLSDYRRIAGSPIQSTFDLLQEFYDAVSERRIEGIRVTGSGSRTIAKIL
                     GIYFENEFKAIARMIGAFYPQVRTVFEIGGESSKYIRLEPQPDHAGTGIVDYDRSGEC
                     AAGTGSFLDQQALRMNYSVEEIGSVVCTASCAARIAGRCSVFAKSDMIHAQQKGYTPA
                     EILRGLCDAVARNFKSSIVKARAAVPPVAFIGAVSQNYGVTAAVREVFKLGENDLVVP
                     ELYAWCGAIGTAILETEERKKRSMRDIHRLDQHESEVRMQESRPLCTGHVLQLRNRVG
                     QYVAPAGDVPIPTYLGIDIGSVSTNVVVMDEFGAVIHDVYLRTAGRPIEAVQQGLTEV
                     YEKWGNRLVIQGVGTTGSGRELIAELVGADVVNDEITAHKTGAVHISNTLGGEMVDTI
                     FEIGGQDSKFISIENGVVVDFAMNEACAAGTGSFLEEQADKLGISIKGEFARLALSSE
                     APTRLGERCTVFMERDVTSWLHKGETVPNLVAGLAYSIALNYLNRVVRGRKIGNVIYF
                     QGGTAYNDAVTAAFASVLGKKITVPPYNGVMGAIGMALVARQWHLATDGKSMFRGYDL
                     SRLKMSSRDFVCKACSNYCDIKEFTIEGHKSYWGDKCSDKFRKPSNTGRKPVIEDLFE
                     YREAVIEEITNSSKPGAPARGIKIGIPRAMATFDRYPFWQRYFSDLGFEVLLSPQTDP
                     KIAAQGVEMALAQPCYPIQVAHGHALALLNAGVDYVLAPNILDAETHEAASCVAHFCP
                     WNQTLPYVLQSAPKLEDHAERFLVPTVHFRLGRECVKKSLAEALRKLGVQRRASDRAV
                     DAAYAAQREFQDRLIEAGRRAIATLETSGEPGLVLVGRGYNIYDRNINCDIPRKLRHR
                     YGANVIPIDFLVTGRELVDDLHPNMYWTSGQKILSAARIVAEARNLHLVYITNFKCGP
                     DSYIKHFTREAAGAPMLVLQFDGHGNDAGYMTRCEAYLDSKGILRCYQSGQEASQLAA
                     STH"
     gene            3567..4778
                     /locus_tag="VLE22_07325"
     CDS             3567..4778
                     /locus_tag="VLE22_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14254.1"
                     /translation="MAYGSGRAFAAVFRAIGLDADIVPPSDDRTRELGARYTSGDECY
                     PAKVTVGDFMKLLEQPDADPSRIALFMPTADGPCRFGQYAPYLRRILDGAGYSNTQVL
                     SPSSKNAYDGLGNLSRPFVRTGWRALLSADILQKLLLKYRPYERQKGETDTVYESCLG
                     DLCSAIEKSPTDPPVQLRAIRDALIRCRDRFRAIPVNRDTTTPLIGVVGEIFCRLHTF
                     SNENLVRCLEEYGGEAWLSDITEWVWYTTAEQFRKLRLEGNLISKEGLGAWIRSQVQH
                     RDEKALVEPFREDFVGYEEPDIHEVLEYAQPYLPQDGAMGEMVLNVGKVVYLAKKGAD
                     GIIDISPFTCMNGIVCEAIYPRLSRDLGGIPIRNFYFDGTQSDLDRDLGVYLELARSY
                     QRRKKHHRPAA"
     gene            complement(4866..6251)
                     /locus_tag="VLE22_07330"
     CDS             complement(4866..6251)
                     /locus_tag="VLE22_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HSB14255.1"
                     /translation="MSKSTDDFRRAGHEIVDWIADYLDNPRKFPVLPDMQPGDLTKRL
                     PSSAPAKGECLDAILSDFHKLVVPAVTHWNHPRFMAYFPSSSSPPGVLGEMLSAALNT
                     NAIVWKSSPANTEMEGVTLSWLRQWLGLPEGFFGIIYDTASVAVVHAIAAAREMADPE
                     VRTKGGSRSLTLYASEHAHSSIEKGAIAVGIGQDNVRKIGTDSEFRMKPGLLREAIEA
                     DRQAGKKPFCVVATVGTTSAGSVDPVIPIAEVAEEYGLWLHVDAAYAGPAAILPERRW
                     ILEGAERAHSLVTNPHKWLLTPSDLSAFFTRRPDILRRAFTLVPEYLRTSQDSVATNL
                     MDYSLAMGRRFRSLKLWFVMRYYGWEGVAGILRRDIAFGERIAKAVDADPRFERVAPV
                     HFSLVCFRYKGSDEENRTLLDRVNATGKAFLSHTVLNGQYVIRFAIGNFNTTQQDIDE
                     IWDLICQSIPG"
     gene            complement(6362..7186)
                     /locus_tag="VLE22_07335"
     CDS             complement(6362..7186)
                     /locus_tag="VLE22_07335"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HSB14256.1"
                     /translation="MRWRRGNVSGDIEDLRSSGGGRRPRVALGLGGLVVLGVLSLVFK
                     TDLISPFMSVGEGTAPSVPDPLRDAREQESVEFVSFVLDDAQSVWGQQMGNYRKAKLV
                     LFRDGVQSACGAAESSAGPFYCPADEKVYLDLGFFDELRTRFRAPGEFAAAYVVAHEI
                     GHHVQNVLGIERRLRALQRQDPSSANELSVKMELQADCLAGVWAHSTAQRQLLESGDL
                     ESGLGAAAAVGDDRIQKEAMGRVHPESFTHGTSAQRMQWFSRGYERGDAGTCETFN"
     gene            7333..9174
                     /locus_tag="VLE22_07340"
     CDS             7333..9174
                     /locus_tag="VLE22_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSB14257.1"
                     /translation="MRLTAVIILAVSLVLPAAGGQAKQDEVAFDYGEVMVPMRDGVRL
                     QTVILTPKDAGEALPFLFERTPYGVPPATAFEKATPLSGMPSGRKYIRVIQNLRGRFK
                     SEGKFVMQRPPHDSTDPKGIDETTDAWDTVDWLVKNVPGNNGRAGIRGTSYSAWTATM
                     AALDPHPAVKAIIEEASPADMFLGDDFHHNGAFRLSYGFEYSAMLETTTEQNYRFQFD
                     RMDTYDWYLGLGPLRNAELRHFRGKIPTWTDFVRHPNFDEFWQRQTVIRYLNAAKAPI
                     LHVAGWWDQEDFYGPQKIYEKLEARDDKSWNFFVAGPWDHGGWHGEGTRLGPITFGSA
                     TGKYYNEKIHDVWFDYWLRSEGSLQLAEATTFETGSNQWCQYEAWPPKRGVTERRLYF
                     GPGRQLSFDPPAKGTGYDEYVSDPANPVPYRPRPVPRTYPGPEWPEWMVQDQRFVDYR
                     PDVLSWETEPLAADLRVVGRLTAELFASTSGTDGDWVVKLIDIYPEDAPPDDTTKKIM
                     GGYQLMIAGEIFRGRFRESFERPKPIPANKPVKYVISLHTNDHVFLKGHRIMVQVQST
                     WFPLYDRNPQRFVPNIFEAGEADYRKAVQRVYRDRQRPSAVVLPVMQ"
     gene            9463..10332
                     /locus_tag="VLE22_07345"
     CDS             9463..10332
                     /locus_tag="VLE22_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB14258.1"
                     /translation="MPLTQAKPIEEIFTPAQTMDMLVPGIESLKSRLRATWMAGDYDR
                     FSRFMENDARSFYERLDVPPGAHLLDVACGSGQLALIAAREGVRVTGVDIAENLIERA
                     QARSTAENLPARFRIADAESLPFSDASFDVVVSLIGAMFAPRPHLVARELTRVCVPGG
                     TIAMANWTAGGFVGSMFKTIAKFIAPSGMPSPVLWGDEATVRERFGAQVFGLTLTRRY
                     FTFDYPFPPSDVVHFFRLYYGPVNRAFSSLDRAGRKSLHQELEALWSAHNRGRDGFTL
                     VAAEYLEVIATRA"
     gene            complement(10410..>11144)
                     /locus_tag="VLE22_07350"
     CDS             complement(10410..>11144)
                     /locus_tag="VLE22_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HSB14259.1"
                     /translation="LLPEGIGREQASGIFDNLSRACRSAGVSLVGGHTEITVGLDRSI
                     LVGCLLGTVRRDRLVRTSGARPGDSVLLAGGIAIEGTAILAREHGAALAARGVAASVI
                     GRAAAWLRSPGISVLPAARAIHATGAAHAMHDPTEGGLVTGLHELSEAAGVGLRIDMA
                     AIPVLPECRLICDALGLDPLGLLASGALIAVLEPAEVGPALDRLRTAGIPGAVIGEVV
                     PGGQSVPVLPVFARDELARYLESQGQ"
BASE COUNT         2147 a         3681 c         3255 g         2061 t
ORIGIN      
        1 ccatcagtaa gttcaacacc tatattctcg agtcggattc cgtcaccggc ccctggcgcc
       61 tcgtcacgta catgaaggac ttcggccagc aggcctactt cgtcaacatc ccctcgaagt
      121 tcatcaaccc ggacgggcgc accttctggc tgatgtacgc cgccaatttc tccaacggca
      181 acaagaactg gcacaccaac caccagcccg atccccccgg cagccgctac ggcatgtgcc
      241 ttcaggaagt ccggcttggg gcgtaggact tctgtcctgc gtccgtgcgg ttgcccgccc
      301 ggcctcttct tttccccgtt caccccccga actcgtccgc ccaatgtaca aaaggtgcat
      361 ttcaggtctg aaatgctttg ttatcaagag gtttgtagta tgatttgatg gcagaggaac
      421 atttccggag cgggcctcat gagtgtgaac atcggcttgg acatcggagc aatcagcctg
      481 aaattggcgg ccctcgggaa accggaagac cgtcccatcc tggcgtccct ctgcgccgac
      541 aagccggcgt tccgcctcat cgactggctg gcccctgacg ggcgccgcct gccgctggtc
      601 ctctccgatt accgccgcat cgccggcagc cccattcagt cgaccttcga ccttctccag
      661 gagttctatg atgccgtttc cgagcgccgc atcgagggca tccgggtcac cggttcaggc
      721 agccgcacca tcgcgaagat cctcggcatc tacttcgaga acgagttcaa agcgatcgcc
      781 cgaatgatcg gggccttcta tccgcaagtc cgaactgtat tcgaaatcgg cggcgaaagc
      841 tcgaagtaca ttcgcctgga gcctcagccc gaccacgccg gcacgggcat tgtggattac
      901 gaccgaagcg gtgagtgcgc cgccggaacc ggctcgttcc tcgaccagca ggcccttcgc
      961 atgaattact ccgttgagga gatcggctcg gtcgtctgca cagccagttg cgccgcccgc
     1021 atcgccgggc gctgctccgt tttcgccaag tccgacatga tccacgccca gcagaagggc
     1081 tacaccccgg cggagatcct gcgcggcctg tgcgatgccg tcgcccgcaa tttcaagtcg
     1141 agcatcgtga aggctcgtgc cgccgtgcct cccgtcgcgt tcatcggcgc ggtctcccag
     1201 aactacggcg tcaccgccgc cgtccgggag gtcttcaagc ttggcgagaa cgacctcgtt
     1261 gttcctgagc tctacgcctg gtgcggcgcc atcggcaccg ccatcctgga gaccgaggaa
     1321 cgcaagaagc gctccatgcg ggacatccat cggcttgacc agcatgaatc agaggtccgg
     1381 atgcaagaga gtcggccgct ctgcaccggt cacgttctcc agcttcgcaa ccgcgtcggg
     1441 cagtacgtcg cccccgccgg cgacgtgccg attcccactt atctcggcat cgacatcggc
     1501 tccgtttcca ccaacgtcgt ggtgatggat gaattcggcg ccgtaattca cgacgtctac
     1561 ctgcgtaccg ccggcaggcc catcgaagcc gtccagcagg gactgaccga agtatatgag
     1621 aagtggggca accgcctcgt cattcaggga gtcggcacca ccggctccgg ccgggagctg
     1681 atcgccgagc tcgttggcgc cgacgtggtc aacgacgaga tcaccgccca caagaccggc
     1741 gctgtccaca tcagcaacac cctcggcggc gaaatggtgg acaccatctt cgaaatcggc
     1801 ggccaggact cgaagttcat ttccattgag aacggggttg tcgtcgactt tgccatgaac
     1861 gaggcctgcg ccgcaggcac tggctccttc ctcgaagagc aagccgacaa gctcggcatc
     1921 agcatcaagg gcgaattcgc ccgcctcgcg ctctcctctg aggcccccac gcgcctcggc
     1981 gagcgctgca ccgtcttcat ggagcgcgac gtcacctcct ggcttcacaa gggcgagacg
     2041 gtgcccaacc tcgtcgccgg tctggcgtat tcgatcgccc tcaactacct gaaccgcgtc
     2101 gttcgcggcc ggaaaatcgg caatgtcatc tatttccagg gcggcacggc gtacaacgac
     2161 gccgtcaccg ccgccttcgc cagcgtcctc ggcaagaaga tcaccgttcc cccgtacaac
     2221 ggcgtcatgg gcgccatcgg catggccctg gtcgcgcggc agtggcatct agctacggac
     2281 ggaaaatcga tgttccgtgg ctatgacctg tcgcggctca agatgagttc ccgcgacttt
     2341 gtctgcaagg cctgctcgaa ttactgcgat atcaaggaat tcaccatcga gggccacaag
     2401 agttactggg gcgataagtg ctcagataag ttccgtaagc cgtctaacac cggccgtaag
     2461 cccgtcatcg aagatctgtt tgaataccgt gaagcagtca tcgaggaaat caccaacagc
     2521 tcgaagccgg gtgcgcccgc ccgcggcata aagattggca tcccccgagc gatggctact
     2581 tttgaccgtt accccttctg gcagcgctat ttcagcgacc tcggttttga ggtcctgctc
     2641 tcgccgcaga cggatccgaa aatcgccgcc cagggtgtcg agatggccct cgcgcagccc
     2701 tgctacccca tccaggtggc acacggacac gccctcgcac tgctcaacgc gggcgtggac
     2761 tacgtgctcg cgcccaacat tctcgacgcc gagacccacg aagccgcctc ctgcgtggcg
     2821 cacttctgcc cctggaatca gacgcttccc tacgtgcttc agtcggcgcc caaactcgag
     2881 gatcacgcgg agcgcttcct cgtgccgacg gtccatttcc gccttggccg cgagtgcgtg
     2941 aagaagtctc tggcggaagc gctgcggaag ctgggcgttc agcgacgtgc cagcgaccgc
     3001 gccgtcgacg ccgcctacgc cgcccagcga gagtttcagg atcggctcat cgaagccggc
     3061 cggcgcgcca tcgccacgct ggagacctcg ggcgagcccg gtctcgtcct cgtcggccgc
     3121 ggctacaaca tctacgatcg caacatcaac tgcgacatcc cgcggaagct tcgccaccgg
     3181 tacggcgcca acgtcatccc catcgacttt ctggtgaccg gaagggaact cgtcgacgac
     3241 ctgcatccga acatgtactg gacgtcgggc cagaagatac tttccgccgc tcgcatcgtg
     3301 gcggaagcga gaaacctcca cctcgtctac atcaccaact tcaaatgtgg gcccgactcc
     3361 tacattaagc acttcacccg cgaggccgcc ggagcgccca tgctggtgtt gcagttcgac
     3421 ggccacggca acgacgccgg ctacatgacg cgctgcgagg cgtatctcga cagcaaaggg
     3481 attctgcgat gctatcaatc gggacaggaa gccagccagt tggcggcgag cacccactaa
     3541 gagggaaaaa ggtctacatc ccgccgatgg cttacggcag cggccgtgca ttcgccgccg
     3601 tcttccgagc catcggcctc gatgccgaca tcgttccgcc ctccgacgac cgcacccggg
     3661 aactcggcgc gcgttacacc tccggcgacg agtgctaccc ggccaaggtc accgttggcg
     3721 acttcatgaa gctgctcgaa cagccggacg cggacccttc gcggatcgct ttgttcatgc
     3781 ccaccgccga cggaccgtgc cgcttcggcc agtacgctcc ctacctccgc cggatccttg
     3841 acggcgccgg atactcgaac acccaggttc tgtctccttc cagcaagaac gcttacgacg
     3901 gtttgggaaa tctgtcgcgc ccgttcgtcc gcaccggatg gcgggctttg ctttcggcgg
     3961 acatccttca gaagctcctg ctgaaatacc gcccctacga acgccaaaag ggcgaaacgg
     4021 atactgtgta cgaaagttgc ctcggcgatc tctgctcggc catcgagaag tcccctaccg
     4081 acccgccggt ccagctacgc gccatccgcg atgccctcat ccgctgccgc gaccggttcc
     4141 gtgcaattcc ggtaaaccgc gacacgacaa ccccgctgat tggcgtggtc ggcgagattt
     4201 tctgccggct ccatacgttc tccaacgaaa atctcgtgcg ctgcctcgag gagtatggtg
     4261 gcgaggcgtg gctctccgac atcaccgagt gggtctggta tacaacggcg gagcagtttc
     4321 gaaagctccg cctcgaaggg aatctcatct cgaaggaggg acttggcgcc tggattcgca
     4381 gccaggtcca gcaccgcgat gagaaggccc tggtcgagcc gttccgcgag gactttgtcg
     4441 gctacgagga gccggatatc cacgaggtgc tcgagtacgc gcagccttac ctaccgcagg
     4501 acggggccat gggagagatg gtgcttaacg tcggcaaggt cgtctatctc gcgaagaaag
     4561 gcgccgacgg catcatcgac atcagcccgt tcacctgcat gaacggtata gtctgcgaag
     4621 ccatctaccc gcgcctcagc cgcgacctcg gtggtatccc gatccggaac ttctacttcg
     4681 acggtaccca gtccgacctc gaccgcgacc tcggcgtcta cctcgagctc gcccgcagct
     4741 accagcgccg caagaagcac caccgccccg cggcatagct gctcactggc tcaaatctgg
     4801 tcccacttcg catgcggtta ccgagccgcg accaaaggga gctgtcaccc cgccggcctt
     4861 attcgctacc ccggtatgct ctggcagatc aggtcccaga tttcgtcgat gtcttgctga
     4921 gtcgtgttga agttgccgat cgcgaagcga atgacatact gcccgttaag tacggtgtga
     4981 gacagaaacg ccttcccggt tgcgttgacc ctgtcgagca gcgttcggtt ctcctcgtcc
     5041 gatcctttgt accggaagca gaccagcgag aagtgcaccg gcgcgacgcg ctcgaagcga
     5101 gggtcggcgt cgacggcctt ggcgatgcgc tccccaaaag caatgtcgcg ccgcaggatg
     5161 ccggcgaccc cctcccagcc gtagtagcgc atgacgaacc acagtttgag cgagcggaag
     5221 cgccggccca tggctagcga gtagtccatc aagttggtcg ccacgctatc ctggcttgtg
     5281 cgcaggtact cgggcacaag ggtgaaggcg cgccgcagga tgtctggccg gcgggtgaaa
     5341 aaggcgctca ggtcgcttgg ggtcaggagc cacttgtgcg ggttcgtcac cagtgagtgg
     5401 gccctttcgg cgccttccaa gatccagcgt cgctctggaa ggatcgcggc cgggccggcg
     5461 tacgccgcat cgacgtggag ccacaagccg tactcttcgg ccacttcggc gatcgggatg
     5521 accgggtcaa cgctgcccgc ggaggtggtc ccaaccgtcg ccaccacgca gaacggtttc
     5581 ttaccagcct ggcggtctgc ttcgatcgcc tctctgagca gaccgggctt catccgaaac
     5641 tcggagtccg tcccgatctt gcgcacgtta tcctggccga tgccgaccgc gatagctccc
     5701 ttttcgatcg aggaatgcgc gtgctcggag gcgtaaagcg taagactccg cgacccgcct
     5761 ttcgtgcgga cctcggggtc ggccatctcc ctggcggctg cgatggcgtg cacgacggca
     5821 accgaggccg tgtcgtagat gatgccgaag aatccctccg gcagaccgag ccactgccgc
     5881 agccagctca gcgtcacgcc ctccatctcg gtgttggcgg gggagctttt ccagacgata
     5941 gcgttggtgt tgagcgccgc cgagagcatc tctcccagaa ccccgggcgg tgaactcgag
     6001 ctcgggaagt acgccatgaa gcggggatga ttccagtgtg tgacggccgg gacaaccagt
     6061 ttatgaaagt cggacagaat tgcgtcgagg cactcaccct tggccggcgc ggaagacgga
     6121 agacgtttgg tcaggtcccc aggctgcatg tcgggaagaa cggggaactt cctggggtta
     6181 tcgagataat ccgcgatcca gtccacgatt tcgtgaccgg ctcgccggaa atcatctgta
     6241 gatttgctca tcggatttga aagatgccgg aggcggcaac cttccagttt ccatcaaatc
     6301 tacagtgcgc tgcagacggt agggagcggc gccccttcga gttctttcgg caccctgccc
     6361 cttagttgaa cgtctcgcac gtgccggcgt cgccccgctc ataaccgcga ctgaaccact
     6421 gcatccgttg tgcggatgtc ccatgcgtga agctctccgg gtggacccgt cccatcgcct
     6481 ccttttgtat cctgtcgtca cccaccgccg ccgccgctcc gagaccgctc tcgagatcac
     6541 ccgattccag aagttgccgt tgcgcagtcg aatgcgccca tacccctgct agacagtcgg
     6601 cctgcaattc catcttcacg ctcaattcgt tcgccgacga agggtcctga cgctgcaagg
     6661 ccctcagccg ccgctcgatt cccaagacgt tctgaacgtg gtggccgatc tcatgggcaa
     6721 ccacgtacgc cgccgcaaat tcgccgggcg cccggaaccg cgtgcgcagt tcgtcgaaga
     6781 atcccaggtc gagatacact ttctcgtcgg ccggacaata aaacggaccc gccgaagatt
     6841 ccgccgcccc gcatgccgac tgtaccccat cccggaacag gacaagcttc gcctttcgat
     6901 aattccccat ctgctggccc caaaccgatt gcgcatcgtc cagcacgaag ctgacaaatt
     6961 ccaccgactc ctgctcccgt gcgtcccgca gtggatcggg tacggacgga gccgttcctt
     7021 caccaacgga catgaacgga gagatcagat cggtcttgaa aaccaggctg agcacgccga
     7081 gtacgactaa gccgcccagt cctagcgcca cacggggcct ccggccgcca ccggatgacc
     7141 gcaaatcctc gatatccccg ctgacattgc cccgtctcca tcgcataagc ccactctaca
     7201 cgcttccggc aacgaaggca caggcaactc gcacgggctt aggatgtcaa tgcagcgtat
     7261 actttgacag gactccgaag ttccacattg aagtctgcgg actatgctcg aacttacctc
     7321 agaggagggc gcatgcgact caccgccgtc atcatccttg ccgtctcgct ggttcttccg
     7381 gctgcgggcg gccaagccaa gcaggacgaa gttgctttcg actacgggga agttatggtc
     7441 cccatgcggg acggtgttcg gctccagacc gtcatcctga cgccgaaaga cgccggggaa
     7501 gcgttaccct tcctgttcga aaggactccc tacggtgtcc ctccggcgac ggcctttgag
     7561 aaggctaccc cgctgtctgg aatgccatcc ggcaggaaat atatccgcgt gatccagaac
     7621 ctgagagggc ggttcaagtc cgagggcaag ttcgtcatgc agcggccgcc tcacgattcc
     7681 acggatccca agggcattga cgaaactacc gatgcatggg ataccgtcga ctggctggta
     7741 aagaatgtgc cgggaaacaa cggcagggcg gggatccggg ggacatcgta ctcggcatgg
     7801 actgccacta tggcggctct cgatccgcat ccggccgtga aagccattat cgaagaggcc
     7861 tcccctgcgg acatgttcct gggcgacgac tttcatcaca acggcgcatt ccgcctgagt
     7921 tacgggttcg agtattcggc catgctcgaa accaccacgg aacagaacta ccgcttccag
     7981 ttcgaccgca tggataccta tgactggtat ctcggcctgg gacctctgag gaacgccgaa
     8041 ttgcgccatt ttcgcggcaa aattcctacg tggacggatt ttgtccgcca ccccaacttc
     8101 gatgagttct ggcaacgcca gaccgtgatc cggtacctga atgcggcaaa ggctcccatt
     8161 ctgcatgtgg ccggctggtg ggaccaagag gatttttacg gcccccagaa gatctacgag
     8221 aagctggagg cgcgcgatga caagagctgg aatttcttcg ttgcgggccc gtgggatcac
     8281 ggcgggtggc acggtgaggg aacccggctc ggaccgatca cgttcggatc ggccaccggc
     8341 aagtactaca acgagaagat ccacgatgtg tggttcgact actggctgcg ctccgagggt
     8401 tcgctgcagc tcgcggaggc aacaaccttc gagacaggga gcaaccagtg gtgccagtac
     8461 gaagcgtggc ctcccaagcg gggcgtcacc gagcgccgcc tctatttcgg tccgggccgg
     8521 cagctttcct ttgacccccc tgcgaaaggt accggctacg acgaatacgt cagcgatccg
     8581 gcgaaccccg tcccctaccg tccgcggccg gtgccccgga cttatcccgg accggaatgg
     8641 ccggagtgga tggtgcagga tcaacggttt gtggactatc gcccggacgt gttgtcttgg
     8701 gagacggagc cgcttgcggc cgacttgcgc gtggtgggcc gcttgacagc cgaattgttc
     8761 gcttctacgt cgggcaccga tggcgattgg gttgtgaaac tcatcgacat ctatcccgag
     8821 gacgctccgc ccgacgacac taccaagaag atcatgggcg gataccaatt gatgatcgcc
     8881 ggcgagatct tccggggccg gttccgcgaa agcttcgaac gtccgaagcc aattcccgcg
     8941 aacaagccag tgaaatacgt catcagcctg cacacgaacg atcacgtgtt cctgaagggg
     9001 catcgcatca tggtgcaagt gcagagtacc tggttcccgc tctacgaccg aaaccctcag
     9061 cgcttcgtgc ccaacatatt cgaggcgggt gaggcggact atcggaaggc agtgcagcgt
     9121 gtctatcgcg atcgccagcg ccctagcgcg gtggttttgc cggtcatgca atagccaggc
     9181 cgaacactct tccaagagcg gggccaacaa cagccacgac atcaagccgg cttctcatag
     9241 cggtgcttca ccgtacaaca cgcggaggcc gggtgccgcg ggttcatgga tccctcggca
     9301 gacgcccggc aaagtgcgga ggacgagcga gcaagtggtt tctatggccg cgccgcccgg
     9361 tcaagaaaac gagacgctcg gataacggtc cccattgaac tgggcctcat agtgggggtg
     9421 gctgatcaag gcagtcaaat ccacttaaga ggggacatac aaatgccact gactcaagca
     9481 aagccgatcg aggagatctt cacgccggct cagacgatgg acatgctggt gcctggcatc
     9541 gagagcctca agtcgcgcct gcgcgccacg tggatggccg gagactacga tcgcttctcc
     9601 cgcttcatgg aaaacgacgc gcgatccttt tacgaacgcc tggacgttcc gcctggcgcg
     9661 catctgctgg atgtcgcctg cgggtctggc caactggcgc tcatcgcggc tcgggagggc
     9721 gtgcgagtga ccggtgtgga catcgccgaa aacctcatcg agagggccca agcccgttcg
     9781 acggcggaaa acctcccggc ccgtttccgg atcgccgatg cagaatcgct tcccttctcg
     9841 gacgcgagct tcgacgtggt cgtgagcttg atcggcgcca tgttcgcgcc gcgcccgcac
     9901 ctggtggcga gagaactcac gcgggtctgt gttcccggcg gaaccatcgc catggcgaac
     9961 tggacggcgg gagggtttgt cggaagcatg ttcaaaacga tcgcaaagtt cattgctcct
    10021 tccgggatgc cttcgccggt gctgtggggc gatgaggcca cggtgcgaga acgtttcggt
    10081 gcacaggtct ttgggttaac cctcacgcga aggtacttca ctttcgatta cccgttcccg
    10141 ccttcggacg tcgtgcactt cttccggctt tactacgggc ccgtgaatcg cgcgttctct
    10201 tctctcgacc gcgccggccg aaagagcctt caccaggagt tggaggcgct gtggtcggct
    10261 cacaatcgag gacgggacgg cttcacgtta gtcgcagccg aatacctcga ggtgatcgca
    10321 actcgtgcct gattgtgctt atgtctcgtg gggcagacga tcggcttctg tcgtctgccc
    10381 ccctcgcaca cgacaacctg ggagatacgc tactgaccct gtgattccag atacctcgca
    10441 agctcgtcgc gtgcgaagac tggcaggact ggcaccgatt gcccgcccgg cacgacctct
    10501 ccgatcacgg cgcctggaat tcccgccgtc cggagccgat ccagagccgg cccaacctcc
    10561 gcaggttcca gcaccgcaat cagcgctcct gatgcgagca ggccgagcgg atcgagaccg
    10621 agcgcgtcac aaatcagcct gcattccgga agcacgggga ttgcagccat gtcgatccgc
    10681 aggcccaccc ccgcggcctc ggacagttcg tgcaatcccg taacgaggcc gccttcagtc
    10741 ggatcgtgca tggcatgtgc ggctccggtc gcgtgaatcg cccgggctgc cggcagcacg
    10801 gaaattcccg gacttcgcaa ccatgccgcg gcacgcccga tcacgctcgc tgcaacgcca
    10861 cgggcggcca gtgccgcccc gtgctcccgg gcgagaatcg ccgtcccctc aatagcgatg
    10921 ccgcccgcca gcagcaccga gtccccgggt ctcgcgccgg aggtccgaac aaggcggtcc
    10981 cggcgaaccg tgccgagcag gcagcccacc aaaatcgaac gatccagccc gactgtgatc
    11041 tccgtatgcc cgcccaccag tgacacgccg gccgaccggc aggcgcggct caggttgtcg
    11101 aagattccgc tggcctgctc gcgcccgatg ccctccggca gcag
//
LOCUS       DATIFQ010000159         2641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_245011, whole genome shotgun sequence.
ACCESSION   DATIFQ010000159 DATIFQ010000000
VERSION     DATIFQ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245011"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..>2641)
                     /locus_tag="VLE22_07355"
     CDS             complement(194..>2641)
                     /locus_tag="VLE22_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14260.1"
                     /translation="PAAIAKEIVWPATFTGIEIAKLKNADPRYFFCDSLAFYMEDLKP
                     VPLQKQPKRNLKPFRGQITGLNTGEGTLPFPTREETILPANFEKDFQVTARQTTPNRF
                     ELQYKGKDAAVTYVYQPKTGNLSELTVAVNAGTPYRPMDGGGVRFTDTPEGQVPQGSL
                     LSATLQDGVVKAKFAIGSRVVDYELRLWQKSLVLDVWCEGGEAVALHFGRVGGVTNPK
                     PLLVPYVTYGGTNPRVLMSGVADKPIFTSIWFDWYRTNASEPYFVKEPKVTSDGAEIN
                     GGMRYINKTDGLRNNLYERIFLTASPVYEEVLPTIANPPSLRQKEGKEVVWTVTSPST
                     FQSDHARCRKIRAYGLDKIMQHSHEVTWRDEGDSFTQRLRAAPQKGGDAMLTWYIAAQ
                     KGLGWTQGVYTNYTDFAPVNSNWNPDHVQRMPDGEWRRAWYRNYALKPSRAVELDEYY
                     AKRIKAKYDNMMSYTDVHTAVSPWNYCDYDARVPGAGTLAATFYAYGQLLLNDQRTYG
                     PSQSEATYQWLYAGLESGSYGWVYTDVNLLTHPLDVSFQVHKMHPLQADYGMGYTNYY
                     LEKLDKDWRKSPKRREYVDLFLATTIGYGNLGWLAGDWGVDDVFGVEAMTRSYYMMQQ
                     LQQQYAFVPPKTIEYADRSGKMVSASLAHSTGAIADSRLHVGYENGTHVYVNRGKEGV
                     WTVKTVDLPVNGWLVFNPANGFYEVSGNVGGRRIDYVKAAEYEFLDGRGQWTERGSLG
                     STGSVARRDRGAGVVELIDIYGNDRISFQADGAGQLMAYDPEGKSLGKVETSSPKTGW
                     HEFKPIKDGRMYIYAGE"
BASE COUNT          480 a          764 c          839 g          558 t
ORIGIN      
        1 gacgagagaa gcgccgtcat cgcgatgact gctgtaatga tgactttcat aaggctcctt
       61 agattctgga agagggcgaa aacctggaaa acccggtaca gacaccaatt tcgcaagagc
      121 ggcgaaattg gagtcagtcc cgggttttcg cacaaccgcg gcgaaattcg gagccggtcc
      181 ccgcttttcg gcattattcg ccggcgtaga tgtacatgcg gccgtctttg attggcttga
      241 attcgtgcca gccggttttc ggcgaggacg tctcgacctt gcccaggctc tttccttcgg
      301 ggtcgtaggc catcaactgg ccggccccat cggcctggaa cgagatgcgg tcgttaccgt
      361 agatgtcgat gagttcgacc actcctgcgc cacggtcacg tcgggcgaca ctgccggttg
      421 agccgagaga gcctcgttcg gtccactggc cacggccgtc caggaattcg tactcagcgg
      481 ccttcacata gtcgatccga cgtccgccga cattccctga tacctcatag aaaccattcg
      541 cgggattgaa gaccagccag ccgttgacgg gaaggtcgac cgtctttacc gtccagactc
      601 cttccttccc acggttcaca tagacgtgcg tgccgttctc gtacccgacg tgcaaccgcg
      661 agtcggcgat ggcgccggtc gagtgggcga ggctggcgga aaccatcttt ccgctgcggt
      721 ccgcgtactc gatcgttttc ggcgggacaa acgcgtactg ctgctgaagc tgctgcatca
      781 tgtaatagga gcgcgtcatc gcctccactc cgaagacgtc atcgactccc cagtcgccgg
      841 ccagccagcc gaggttcccg taaccgatcg tagttgcgag gaacaggtct acgtactcgc
      901 ggcgcttcgg ggacttccgc caatccttgt cgagcttttc gaggtagtag ttcgtgtacc
      961 ccatgccgta atcggcctga agcgggtgca tcttgtgcac ctggaaagag acgtcgagcg
     1021 gatgggtgag gaggttaacg tcggtgtaga cccagccgta gctgccggat tccaggcccg
     1081 catagagcca ctggtaggtg gcctcggact gggacggtcc atacgtccgc tggtcgttga
     1141 gaagaagctg gccgtaggcg tagaacgtag ccgcgagggt acccgccccg ggcacgcggg
     1201 cgtcgtagtc gcagtagttc cacggcgaga cagcggtgtg gacgtcggtg taggacatca
     1261 tgttgtcgta cttcgccttg atccgcttgg cgtagtactc atccaactcc acagcccgag
     1321 agggcttaag cgcatagttt cgataccagg cgcggcgcca ttcgccgtcc ggcattctct
     1381 ggacgtgatc gggattccag tttgagttaa ccggggcgaa gtcagtgtag ttggtgtaaa
     1441 ctccctgcgt ccagccgagg cccttctgcg ccgcgatgta ccacgtcaac atggcgtcgc
     1501 cgcccttctg gggagcggcc cggaggcgct gtgtgaaact gtcaccctcg tcgcgccagg
     1561 tgacctcgtg gctgtgctgc ataatcttgt cgaggccgta ggcgcggatc ttccggcagc
     1621 gtgcgtgatc ggactggaaa gtggatggcg aggtcacggt ccagacgact tctttgcctt
     1681 ccttctgccg gagcgacggt ggattggcaa tggtcggcag cacctcttcg tagaccggcg
     1741 aggccgtaag gaagatgcgc tcgtagaggt tgttgcggag gccatcggtc ttgttgatat
     1801 accgcatgcc gccgttgatc tcggcgccgt cggaggtcac cttcggttcc ttgacgaagt
     1861 aaggctcgga ggcgttggtg cggtaccagt cgaaccagat cgacgtgaaa attggtttgt
     1921 cggctactcc ggacatcaac acgcgcgggt tcgtgccgcc gtaggtgacg tacgggacaa
     1981 gcagcggctt ggggttggtg acgccgccga cgcggccgaa gtgcagggcc accgcttctc
     2041 cgccttcaca ccagacgtcg agcaccagcg acttctgcca gaggcgcagc tcatagtcaa
     2101 cgacacgaga gccgatcgcg aacttggcct tgaccacgcc gtcctggagc gttgcggaaa
     2161 gcaaggagcc ttgcgggacc tgcccctcgg gcgtgtctgt gaaccgtact ccgccgccgt
     2221 ccatcggacg gtacggcgtt ccggcgttga cggcgaccgt caattcactc aagttgcccg
     2281 ttttcggctg atacacgtag gtgacggcgg catccttgcc tttgtactga agttcgaagc
     2341 ggttcggcgt ggtctggcgg gcagtcacct ggaagtcctt ctcgaaattg gccggaagga
     2401 tggtctcctc acgagtcggg aagggcagtg tgccttcgcc ggtgttgagg ccggtgattt
     2461 ggccacggaa gggcttcaaa ttccgcttgg gctgcttctg gagcgggacc ggcttaagat
     2521 cctccatgta gaaggcgaga gaatcgcaga agaagtatcg cggatcggcg ttcttcagtt
     2581 tggcgatctc gatacccgtg aacgtcgccg gccagacgat ttccttggcg atcgcggcgg
     2641 g
//
LOCUS       DATIFQ010000160         2943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1394156, whole genome shotgun sequence.
ACCESSION   DATIFQ010000160 DATIFQ010000000
VERSION     DATIFQ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394156"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..539)
                     /locus_tag="VLE22_07360"
     CDS             complement(201..539)
                     /locus_tag="VLE22_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14261.1"
                     /translation="MKPREWYRAEIRWAVMEDKQGLRSWEDAVYFFLSEDHEAAFQHA
                     LEIGMRERDAHEEGRRWVEKRLARIVSLDWMGADPTEFEVRLGSQRAKERLPFEHEFD
                     PEGTQPMSVF"
     gene            complement(536..931)
                     /locus_tag="VLE22_07365"
     CDS             complement(536..931)
                     /locus_tag="VLE22_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB14262.1"
                     /translation="MEACRQRKRVRFTTAAELVNELIEGKNRSELNRITNRWLRYELI
                     VIDEMAYVAMPEAAAELLFQVISGRAERAAVIVTTNLPFSEWTTMFPNARLCKAMLDR
                     LTDQAHIIDTGKESYRFRRTMERKKGGKG"
     gene            complement(874..1320)
                     /locus_tag="VLE22_07370"
     CDS             complement(874..1320)
                     /locus_tag="VLE22_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB14263.1"
                     /translation="MSASTAQLQQSTIRQYTLQLRLPTVGGQFLKMAEEAGKQKLGHV
                     AYLEALLAAEVEERERNAVARRIQESKFPKVKTLEDFHFDEAPLIPAAQIRKLAEGGY
                     LSRSEPIVLVGETVKQARGKRTWRPDWQWKPVGSGSGCDSRRRPSW"
     gene            complement(1317..2870)
                     /gene="istA"
                     /locus_tag="VLE22_07375"
     CDS             complement(1317..2870)
                     /gene="istA"
                     /locus_tag="VLE22_07375"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HSB14264.1"
                     /translation="MRRRKSKVELFEQIRREYEHGVGTIQGVARKFGVHRRMVREAID
                     SAVPRPRKKAEREKPKMAAVIPWIEEILEADQKAPRKQRHTAHRIWCRLQLERPEADV
                     GESTVREYVRQRKIELGLEHGEVFVPQTYHWGQEGQVDWYEAWAEIDGEQVKAYVFCL
                     RSMASGGAFHRAYPHASQQAFLEAHELAFAYFGGVFRVLRFDNLKSAVKQILRGHQRE
                     ETERFIAFRSHWRFESEFCTPGEGHEKGGVEGENGYYRRNHLVPLPKARSWDELNQFL
                     LEECKRDEQRVIGDRSQSVGAGMCQEREHLLPLAEEGFELASTHFPKVNGSRCVRVLT
                     NFYSVPAPVGREVQAKVYAAHVEIWNEGKCIARHERCFSRQQKVLDLEHYLDVLSKKP
                     GALAGSTPLEQWRAQGRWPVSFDRFWEALKQRRGKRDGTRAMIDLLLVGREQGYDALR
                     HAIEKTLEMGCSDVSAVLLLLRAGQAPQRGEPEPVEIGSLRGYDRPQPTTIDYDLLLQ
                     KWPETGVVQ"
BASE COUNT          495 a         1050 c          734 g          664 t
ORIGIN      
        1 gcgttatggt tgtttctctg cgcgggaggc gttgctgccc aggtcggttt gtcaacttga
       61 tttggcccac ttcgatgctt tcacttggcc cacctcgtga gccaatgatt cctctggcgc
      121 cggctggagc ggagccctgc gccgcggttt tggcggcgcg ttagggcgta gcggaggccg
      181 gcgtcagagg aatcagaccg tcagaagacg ctcatcggtt gcgtaccttc cgggtcaaac
      241 tcgtgttcga acggcagccg ttctttggcc cgctggcttc cgagtcgcac ctcaaactcc
      301 gttggatccg cccccatcca atccaggctg acgatccgtg ccaggcgttt ttctacccac
      361 cgccgccctt cttcgtgggc atcacgctct ctcatgccga tttccagcgc gtgttggaat
      421 gcggcctcgt ggtcctcact caagaagaag tagacggcat cttcccagga ccgcaagcct
      481 tgcttgtcct ccattaccgc ccatcggatc tccgctcgat accactctcg cggcttcatc
      541 ctttacctcc cttctttctt tccatcgttc gccgaaaccg gtacgattcc ttccccgtgt
      601 cgatgatgtg cgcctggtcg gtcagccggt ccagcatcgc cttgcacaat cgggcattcg
      661 gaaacatggt cgtccattcg gaaaatggaa gattcgtggt tacaattacg gccgcccttt
      721 cggctcgccc tgagatcact tggaacagca gctcggccgc cgcttccggc atcgccacat
      781 acgccatttc atcgatgaca atcagctcat acctcagcca gcggttggtg atccggttga
      841 gctcgctgcg attcttgcct tcgatcagtt cgttcaccaa ctcggccgcc gtcgtgaatc
      901 gcacccgctt ccgctgccga caggcttcca ctgccagtcc ggtcgccaag tgcgttttcc
      961 ccgtgcctgt ttcaccgttt caccaaccaa aacgatcggt tcgctccggc tcagatagcc
     1021 gccttccgcc agcttgcgga tttgtgcggc cggaatgagc ggggcttcgt cgaagtgaaa
     1081 gtcttccagg gtttttactt tcgggaactt cgattcctgg attctccgtg ccaccgcgtt
     1141 ccgctcccgt tcctccacct cggccgccag caaggcttcc agatatgcca catgcccgag
     1201 tttctgcttc ccggcttctt ccgccatctt cagaaactgc ccgcccaccg tcggcagccg
     1261 caactgcagc gtgtactgtc gaatcgtgga ttgctgcaac tgcgctgtgg atgcgctcat
     1321 tgcaccaccc cagtttccgg ccacttctgc aataacaggt cgtaatcgat ggtagtcggc
     1381 tgcggacggt cgtagcccct caagctgccg atctccaccg gctcgggctc gccacgctgc
     1441 ggagcctggc cggcacggag cagcaacaac accgcactca catccgaaca gcccatctca
     1501 agagtcttct caatggcgtg ccgcaaagcg tcgtatccct gctcgcggcc taccagcagc
     1561 aggtcaatca tcgcccgggt gccgtcccgt ttcccccgcc gctgtttcag cgcctcccag
     1621 aaacgatcga aactcaccgg ccaccggcct tgtgcacgcc actgttctaa tggcgtggag
     1681 ccggccagcg cgcccggttt cttactcagc acgtccaagt agtgttccag atcgagcacc
     1741 ttctgttgcc ggctgaaaca ccgctcgtgc cgggcaatgc acttcccctc attccagatc
     1801 tccacgtggg cggcgtagac tttcgcctgt acctcccgac cgaccggcgc tggaaccgaa
     1861 tagaagttgg tcagcactcg cacacacctg ctcccgttca ccttcggaaa atgcgtcgag
     1921 gccagctcga agccttcctc ggccaaaggc aacaggtgct cccgttcttg gcacatcccc
     1981 gcgcccacac tctggctgcg gtcgccgatc acgcgctgct catcccgttt gcattcttcc
     2041 agcagaaact gatttaactc gtcccaactc cgcgccttcg gcaacggcac caggtgattc
     2101 cgccggtaat atccgttttc cccttccacc ccgccttttt cgtgcccttc tcccggcgtg
     2161 caaaactcgg actcgaacct ccagtgcgac cggaaggcaa tgaaccgctc ggtctcctct
     2221 cgttgatgac cccgcagaat ctgcttcacc gcgcttttca gattgtcgaa acgcagtacc
     2281 cgaaataccc cgccaaagta ggcgaacgcc aactcatgcg cctccaaaaa tgcctgttgg
     2341 ctggcatgtg gatacgcccg gtgaaaggct cccccgctgg ccatgctccg cagacagaat
     2401 acgtaagcct tgacctgctc gccgtcgatc tcggcccagg cttcgtacca gtccacctgc
     2461 ccttcctgcc cccagtgata cgtctgcggc acgaacactt ccccgtgctc cagacccagt
     2521 tcgatcttcc gctggcgcac atattcccgc accgtagact cccctacatc ggcctctggc
     2581 cgctccaact gcaaccggca ccagatgcgg tgcgccgtgt gacgctgttt ccgcggtgcc
     2641 ttctggtccg cttccaagat ctcctcgatc cacgggatca ccgccgccat cttcggcttc
     2701 tctcgctccg ctttcttccg cggccgtggc accgcgctat cgatcgcttc gcgcaccatc
     2761 cgccggtgga cgccgaactt ccgcgccaca ccttgaatcg tcccgacgcc gtgctcgtac
     2821 tcgcgccgga tctgctcgaa tagctccacc ttgctcttcc tccttctcaa accggctccc
     2881 ttccgggccg tccggcccag aacagaatag tcggtttatg tcgttaaggg gtgggccaga
     2941 tca
//
LOCUS       DATIFQ010000161         2543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1012028, whole genome shotgun sequence.
ACCESSION   DATIFQ010000161 DATIFQ010000000
VERSION     DATIFQ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012028"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VLE22_07380"
     CDS             complement(<1..620)
                     /locus_tag="VLE22_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HSB14265.1"
                     /translation="MTDVVMPQMGESIVEGTLTKWLKKPGERVERDEPLFEISTDKVD
                     TEIPAPSAGVLAEVMVEEGKTVAINTVVARIDESGDGKVTVAPPKPVEKTKPEAPPVP
                     APAVAQPTPAAAPAVAVPAAAPEEVAGPLSPLVRKMAREYNIDLRQVKGTGAGGRITK
                     QDAEAYIAAQAARTVAAPPPPAPVAAPVMPPPPAVPTEMPPVPRPEP"
     gene            complement(617..1285)
                     /gene="lipB"
                     /locus_tag="VLE22_07385"
     CDS             complement(617..1285)
                     /gene="lipB"
                     /locus_tag="VLE22_07385"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HSB14266.1"
                     /translation="MRKCERRDLGRIGYGEAFAVQQSLVEQRKRGEICDQFLIVEHPH
                     TITLGRNGKMEHLLAAEEALRRAGIAFHASDRGGDITYHGPGQIVGYPIVDLREWKRD
                     VVAYVRALEEVLIGALAEFGIGAERIPGCTGVWAGKKKIAAIGVHISRWVTSHGFALN
                     VNTDLSYFQYIVPCGLSKPVTSMAESGSSAGRAQVAEALARHFARVFDCEIVSAETEM
                     EEVL"
     gene            complement(1278..>2543)
                     /gene="lpdA"
                     /locus_tag="VLE22_07390"
     CDS             complement(1278..>2543)
                     /gene="lpdA"
                     /locus_tag="VLE22_07390"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HSB14267.1"
                     /translation="VGCIPTKALLHTADLWEEILHGKDQGVLCDNPRLDYPKTVERKN
                     QVVAKHAKGIEFLMKKNKVEWIKGQATLKGGGKVEVQTDAGVKTIEAKNVIIATGSEA
                     RMLPGLTPDPDSILTNIEILDMTAIPKTLAIVGAGAVGVEFASIFNRFGTKVTLLEML
                     PRIVPLEDEEISKELQRLFKKSGIRVETGAKADNIRKTGDGVRFSVTLADGKKEDMQA
                     DRILIAVGRKPNTENVGLENTRVKLDRGFIKVNEWMQTDEPGVYAIGDVVAGSPLLAH
                     VATMEGMVAVAHIAGRPAQPIRPGRIPNCTYTEPGIGSVGMTEAQAREKGYRLKIGKF
                     PFMANSKATILGRHDGFVKVVADEKHGEILGVHIIGPMATDLIAEAVAAMEAEATVET
                     LMHTIHPHPTVSEAMGEAFNAVYGLAINA"
BASE COUNT          383 a          871 c          739 g          550 t
ORIGIN      
        1 ggctcgggcc tcggcaccgg cggcatctcg gtgggaactg ccggaggcgg gggcatgacc
       61 ggtgcggcga ccggggcggg tggcggcgga gccgcgaccg tacgagccgc ctgggcggcg
      121 atgtaggctt ccgcgtcctg cttcgtgatc cgccctcccg cgcccgtgcc ctttacttgc
      181 ctcaggtcga tgttgtactc gcgcgccatc ttccggacca gtggtgacag aggcccggcg
      241 acctcctccg gtgccgctgc ggggacagct acggccggcg ccgctgcggg agtgggctgt
      301 gcaaccgccg gcgcaggaac gggtggcgct tccggcttgg ttttctcaac cggcttcggc
      361 ggagcgacgg tcacctttcc atcgccgctt tcgtcgattc gcgccaccac cgtgttgatg
      421 gcaaccgttt tgccttcttc caccatgacc tcggccagaa cgccggctga cggcgccgga
      481 atctcggtgt caaccttgtc ggttgagatc tcgaacagcg gctcgtcccg ctccacccgt
      541 tctcctggct tcttcagcca cttcgtcagc gtcccttcta cgatgctctc tcccatctgc
      601 ggcattacga cgtcggtcat agaacttcct ccatctccgt ttctgctgat acgatctcac
      661 agtcaaacac gcgggcgaag tgccgcgcca gcgcttcagc cacttgggcg cgcccggcgc
      721 ttgaacccga ttcggccatc gacgtgacgg gtttgctcag cccgcacggc acgatgtact
      781 ggaaataact caggtccgtg ttcacgttga gcgcaaaacc gtgggaagtc acccagcggc
      841 tgatgtgtac cccgatcgcg gcgattttct tcttgccggc ccacactccg gtgcagcccg
      901 gaatccgctc cgcgccgatg ccgaattcgg ccagcgcccc gatcaggacc tcctcgagcg
      961 cccgcacgta cgccacgacg tcacgtttcc actcgcgcaa gtcgacaatc gggtatccaa
     1021 cgatctgccc gggcccgtgg tacgtgatgt cccccccacg gtccgacgcg tgaaacgcga
     1081 tgccggctcg ccgcagggct tcctcagccg caaggaggtg ctccatcttt ccgttgcggc
     1141 ccagcgtgat cgtgtgaggg tgctcgacga tcagaaactg atcacagatc tctccgcgct
     1201 tccgctgctc gacaagactc tgctgcactg cgaacgcttc gccgtagccg atccgtccca
     1261 ggtcgcgacg ctcgcactta cgcattgatc gccagaccgt ataccgcgtt gaacgcctcg
     1321 cccatcgcct cggagaccgt tgggtgcggg tgaatggtgt gcatcagcgt ttcaaccgtc
     1381 gcttccgcct ccattgccgc cacggcctcc gcgatcagat cggtggccat aggcccgatg
     1441 atgtgcacgc cgaggatctc gccgtgtttt tcgtccgcta cgaccttaac aaagccatcg
     1501 tgccggccca ggatcgtcgc cttgctgttc gccatgaagg ggaacttgcc gatcttgagc
     1561 cggtagccct tctctctcgc ctgcgcctcc gtcattccga cgctgccgat tcccggctct
     1621 gtatacgtgc aattcgggat gcggcccggg cgaatgggct gggccggtct gcccgcgatg
     1681 tgggcgaccg ccaccatccc ctccatggtt gccacgtgcg ccagcagggg gctgcctgcc
     1741 acgacatctc cgatcgcgta cacccctggc tcgtccgtct gcatccactc gttgaccttg
     1801 atgaagccgc ggtccaattt cacgcgggtg ttttccagcc ccacgttttc cgtgttcggc
     1861 ttgcggccga cggcgatcag gattctgtcg gcctgcatgt cttccttctt gccgtcggcg
     1921 agagttaccg agaagcggac gccgtcaccc gtcttccgga tgttgtccgc cttcgcgcca
     1981 gtctccaccc gaatgccgga tttcttgaac aggcgctgga gttccttcga aatctcctcg
     2041 tcttcgagcg gcacgatgcg cggcagcatc tcgagcagag tgaccttcgt cccgaaacgg
     2101 ttgaagatcg aggcgaactc aacgccgacc gcgcccgccc cgacgatggc cagcgtcttc
     2161 ggtatggcgg tcatgtccag gatctcgatg ttcgtcagga tcgagtccgg atcgggtgtg
     2221 agcccgggca gcatacgcgc ctcggacccg gtggcaatga tgacgttctt cgcttcgatc
     2281 gtcttcacgc cggcgtcggt ctgtacctct accttgccac cgcctttcaa ggtcgcttgg
     2341 cccttaatcc attcgacctt gttcttcttc atcaggaact caatgccctt ggcgtgtttt
     2401 gcgacgacct gattctttcg ctccacggtc ttcgggtaat cgagacgcgg gttgtcgcag
     2461 agaacgccct gatctttccc gtgcaggatc tcttcccaaa ggtccgcggt gtgcaagagt
     2521 gccttcgttg gtatgcatcc aac
//
LOCUS       DATIFQ010000162         2761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2352859, whole genome shotgun sequence.
ACCESSION   DATIFQ010000162 DATIFQ010000000
VERSION     DATIFQ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2352859"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VLE22_07395"
     CDS             complement(<1..562)
                     /locus_tag="VLE22_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14268.1"
                     /translation="MRHQNLDAALALAEKGLKPFQEKQESPWHWRFLLLKAEILTGKG
                     RAADALKLLDRSTPGPPDAELSARLKMQQGYATFRLSRYDESLRLLDEALRIAADLNS
                     PALVAEIQNKRGPPLVRLVQPAKAEKCFRDALEQARRAGDSYLEVSALGNLGSTRLFT
                     GRFDEAILSFEQMLPLARKGGSPRIVG"
     gene            complement(658..1422)
                     /locus_tag="VLE22_07400"
     CDS             complement(658..1422)
                     /locus_tag="VLE22_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14269.1"
                     /translation="MPNPRKFIYSASAVAVAAKFDGPHALNRAWATVALPMIGGSDIS
                     TEKGPNGNPVLSFASATASVKGSENSGVFTTINTVTVEGLNILSGWLEADLELTLRFD
                     YADGNPAVLGAVVSNQPFTRLVVGGQDFGNVTLDNEPVKQAAKDHDKFRNDLKQGTIL
                     KDKHHINKSKKNKKELKAHASIAVDPARLKFDPATGEYGYVGAPERRVYIGEWAEDED
                     WQGIVGLRIVFESTNPQAKGEIVIADWLGDNGQFYP"
     gene            complement(1430..2134)
                     /locus_tag="VLE22_07405"
     CDS             complement(1430..2134)
                     /locus_tag="VLE22_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSB14270.1"
                     /translation="MEHQEAQRTQAAERYLLGEFSADEREEFEEHYFSCPECAEEVTT
                     GALLRANTAAVLRDEAQISTEVRVRPQFGWLAWLGLRPAFSVAWAAACVLFAGVAVYQ
                     TAFVVPRLNVQLAAARAPQVFQDVVLRPLSRGEDPALRLAQAGEYAVVKVPLPPQAEF
                     VTYRAQILNESGILLHTVDAAPPAVPGDPLQFLVHSSSVPPGTYTLVVFGRRDPAEPA
                     PGARLETYRFVVKQDK"
     gene            complement(2217..>2761)
                     /locus_tag="VLE22_07410"
     CDS             complement(2217..>2761)
                     /locus_tag="VLE22_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB14271.1"
                     /translation="EYIQRLKQGDPFVEEHFNAYFGELIFLKLRNRLRTPQLMDDIRQ
                     ETLLRVIRTLRTGTGIEFPERFGAYVNSVCNNVTHELLRQNGRFEPFNNDASEPVDTR
                     VDLDLPLITEDRKRLVHKVLEELPSRDREILKLLFLEERTPSDVCQRLKVDAGYLRVL
                     LHRAKSRFKSRLGAAGAVGD"
BASE COUNT          525 a          823 c          854 g          559 t
ORIGIN      
        1 gaccgacgat gcggggtgag ccgcccttcc gcgcaagcgg cagcatctgc tcgaagctga
       61 gaatcgcctc gtcgaatcgt ccggtgaaca gccgcgtgga tccaaggttg ccgagcgcgg
      121 agacttcgag atacgaatcc cctgcgcggc gggcttgctc cagcgcatcc cggaaacact
      181 tctccgcctt ggcgggctgg acgagccgga cgagcggcgg cccacgcttg ttctggattt
      241 cggcgaccag ggccggtgag ttcagatctg cggcgatccg gagcgcttca tccagtagac
      301 gcaggctctc gtcgtatcgg ctgaggcgaa acgtggcgta gccctgctgc atcttcaagc
      361 gggcggaaag ttcggcgtcg ggagggccag gagtggaccg gtcaaggagc tttagggcgt
      421 ccgctgcccg gcctttcccg gtgaggattt cggctttgag aagcaggaac cgccagtgcc
      481 acggcgattc ctgcttctct tggaagggct tcaggccttt ctcggcgagg gcgagagcgg
      541 catcgaggtt ctgatgccgc atctcgcgtt gagcttcttg atacagcttg tcaggtttct
      601 cccccagacg gcaggcacaa gccgccagca acaggatcaa agccggaccc caccgcatta
      661 tggatagaac tggccgttgt caccgagcca atcggcgatg acgatttcac ctttggcctg
      721 cgggttcgtg ctttcgaaga cgattcggag gccgacaatg ccctgccaat cctcgtcctc
      781 cgcccactca ccgatgtaga cgcggcgctc tggagcgccg acgtagccgt actcaccagt
      841 ggccgggtcg aatttcaggc gtgcagggtc cacggcgatg ctggcgtggg ctttgagttc
      901 cttcttgttt ttcttcgact tgttgatgtg gtgcttgtcc ttcagaatgg tgccttgctt
      961 cagatcgttc cggaacttgt cgtggtcctt tgcggcctgc ttgacgggtt cgttgtccaa
     1021 tgtcacattg ccaaagtcct ggccgccgac gactagccga gtgaaaggct ggttgctgac
     1081 gacggctcca aggacggctg ggttaccatc cgcgtaatcg aatcgtaggg tcaattcgag
     1141 gtctgcctcc aaccagccag acagaatgtt caggccttcg acggtgacag tgttgatcgt
     1201 tgtgaagacc ccggagttct cggagccctt cacgctggcg gtcgcagacg caaaagagag
     1261 gaccggattt ccgtttggcc ccttttcggt ggagatgtcg gagccgccga tcatgggaag
     1321 tgcgacggtc gcccaagctc gattgagcgc atgtgggccg tcgaacttcg cggccacggc
     1381 aacggcgctt gcgctgtaga tgaattttcg cgggttcggc atttttgcct cacttgtctt
     1441 gtttgacgac aaagcggtac gtctccagtc tggcgccggg ggccggttcg gccggatcgc
     1501 ggcgcccgaa aacgactaag gtatacgtgc cgggcggcac gcttgaagag tgaaccaaga
     1561 attgcagggg atcgcccggg acggccggag gagccgcgtc gaccgtatga agcaatatac
     1621 cggattcgtt caggatctga gcccgatagg tgacgaactc ggcttgcggc gggagaggta
     1681 ctttcaccac cgcgtattcg cccgcctgag cgagacggag tgccgggtcc tctccacgcg
     1741 aaagggggcg caggacaacg tcctggaaaa cctgcggcgc ccgcgccgcg gccaactgga
     1801 cattgagcct ggggacgaca aacgccgtct ggtagacagc cacgccggcg aacaggacgc
     1861 acgcggcggc ccaggcgacc gaaaatgcag gccgtaaccc cagccacgcc agccagccaa
     1921 actgcggccg gacccgtact tccgttgata tctgcgcctc gtcgcggagg acggccgccg
     1981 tattcgcccg caagagcgcc ccggttgtta cctcctcggc gcactccgga caggaaaagt
     2041 aatgctcttc gaactcttca cgctcatcgg cggaaaactc acccagcaga tatcgttctg
     2101 ccgcctgggt tctctgagcc tcttgatgct ccatgacatt tgcctctacc agatggtgat
     2161 gctcgcaacc acttcatttc acgcgatcaa acaaaatatc gcgtccggag ccccggtcag
     2221 tcaccaaccg cgccagccgc tcctaacctg ctcttgaatc gagacttagc gcggtgaagc
     2281 aatacccgga gatagcccgc gtcgactttg agccgctggc agacgtccga cggcgtccgt
     2341 tcctcgagaa aaagtagttt caggatttcg cggtcgcgcg aggggagctc ttcgagcact
     2401 ttgtgaacga gccgtttccg atcctcggtg attagcggca gatccaagtc gacccgcgta
     2461 tcaaccggct cggacgcgtc attgttgaac ggctcgaacc tcccattttg ccggaggagt
     2521 tcgtgggtca cgttgttaca gacggagttc acataggcac cgaagcgttc gggaaactcg
     2581 atcccggtcc cggtccgcag ggtgcggatc acgcggagca aagtctcctg acggatatcg
     2641 tccatcagtt ggggcgtacg gagacggttc cggagcttca ggaaaatcag ttcgccgaag
     2701 taggcgttga aatgctcctc gacaaacggg tccccctgct tgagccgttg aatatattcc
     2761 g
//
LOCUS       DATIFQ010000163         5805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_53798, whole genome shotgun sequence.
ACCESSION   DATIFQ010000163 DATIFQ010000000
VERSION     DATIFQ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53798"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VLE22_07415"
     CDS             <1..432
                     /locus_tag="VLE22_07415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSB14272.1"
                     /translation="WKSDFLLPLAGFAISAPVLMVVGLELGFIPPPHLPAQTAARMAG
                     AAGLIFVGTALPEEILFRSLVQNWMMLRFGDNIRTLLAASFLFGCAHLDNGPQPLPNW
                     RYMILATIAGIAYGKVFQRSSTVLSSSGLHTLVNWTKHSFF"
     gene            439..1056
                     /locus_tag="VLE22_07420"
     CDS             439..1056
                     /locus_tag="VLE22_07420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSB14273.1"
                     /translation="MYRKLAILEPLAVFGLIVAYIWGLRAMYPNLWIAILGLMLLSHL
                     LHGESPGLLGFGTRNLQGVMREIAPPLAFLALLMLACGILLRTIRPISVDGAVVALAG
                     YLPWGLLQQYILNGYFLNRLDAVLSPRAAVVITAMLFCAAHAPNWFLMGVTLPGSYWA
                     TRVYQRRRNLYVMGLAHAIVGFLLFLVVPDSITHHLRVGPAWFSR"
     gene            complement(1108..1509)
                     /locus_tag="VLE22_07425"
     CDS             complement(1108..1509)
                     /locus_tag="VLE22_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB14274.1"
                     /translation="MHFGLFEADLATGELRKKGRRVRLQQQPFQALALLVQRPGEVVT
                     RKELEKALWPDGTFIEFDQGLNAAIKKARSALCDSPRNPRFIETVPRRGYRFIADVHV
                     NRETARRRVAAWAMMLFGLVRSTAVLLMPDR"
     gene            complement(1617..2381)
                     /locus_tag="VLE22_07430"
     CDS             complement(1617..2381)
                     /locus_tag="VLE22_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14275.1"
                     /translation="MTRFGKASLLVIPAMAATLLAPLPTRSSAAPRTGCQDLPISYTF
                     LSTTAAPAAIWNDDPTKPYENGSIHRCGSNPTNDATLVLAHSKRSVWIRVPNPFPNSN
                     IVNPPPSFAGGAPFQSQAFFNVRNLLGSKDPYFYTSMASQFPAPDGKTHHLRLLPDAW
                     ACPGDLVCVPNIHGGILADSNLPVETSWVRVHYMPPPNPSQPWSLSNTAQWIVDGELA
                     DTVYPSLYERATLHNGTHEGQYSMPLQILITALGPL"
     gene            2722..>5805
                     /locus_tag="VLE22_07435"
     CDS             2722..>5805
                     /locus_tag="VLE22_07435"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB14276.1"
                     /translation="MSIPRCFPAFALLAGVVASAPSLCAQSTFGSIVGSVKDLSSAVV
                     PAATIAARDVEENTTRTTTSNDQGLYELLNLKPGRYEITATKAGFSTFTIRAVMLLAR
                     DTVRADVRLEVSPIKESVAVTGEASIINTENGTISDTKSFREITGLPLNYRGVTTSPL
                     IALISVPGVQMSLLDRPSIGGGLPAQIEYSLDGISTVNIAFFGPIYDMYPSTEMISEF
                     KVISVNNSAEYGQMGDVTVITRGGTNQLHGSAVWYHQNSALDATIYGAPEKQSKVFNT
                     FGGSLSGPLYLPGLYHGRDRTFFFVDYEGNRKPSAVVEHLSVPTASMRAGDLNGVPGQ
                     SASDPLSGAPFPNNAIPATRINSVARTLFQRYYPLPNFNTDGTTFDNYRSVVPTSIYT
                     DGYDVRVDHVLNSRHQIFGRWSWKEISGKGAYELLPPEQYDSANRNLILSHNFVLRPN
                     VFNESRFGFGLSDGRTRFPILGADAVATLGLEGLNLSNAGGVGGFPLFDFSEGTGFYL
                     IGHPRDGPGRSRNWQFTDNLSWIRGRHSMKFGADIRRIGWVGVLSDGGGDDFGSFLFQ
                     PDAFSGNAFVDLLLGLPYLTEYGLLGPNLDESATHAHFYGQDQWQVNDRLTVSIGIRW
                     SVHPPMTEASGNITNFDPASGNVIVPDHTIPAAPGFLTGINACPGVTTAFPCTKIVTA
                     SEAGVGQGLRRTYYGNWAPRVSIAWRPFASNKTVVRSGFGEFTQTILGATASGLTAIH
                     STDLRDEFNYQGPGVAPQFVLPLVASGAFVLPQPGTKTFGDGMDTSYKDPRSYQWNFT
                     LEHALAAETRLRLSYIGSHSVGLNGLVNYNQQHASTTPFSASRRPYPAFRTVGMWENI
                     GFASYEAFQAEATRRFARGLFFQASYIFSKNIGNAGSAYGGQGGLLFVPEALPNAVTD
                     RFNTRLDRGILAGSRANRFLFTGIYELPWGKGRRYANRLPALPNALFGGWNLSTITLL
                     ESGPFQTAFTRAPDPSNSGAGGRLKRPDRIGDGNLPHPTPDHWYDITAFVPTPQGA"
BASE COUNT         1100 a         1744 c         1723 g         1238 t
ORIGIN      
        1 tggaagagcg atttcttgct gccgcttgcc ggttttgcga tctcggcgcc agtcctcatg
       61 gttgtggggc tggagctcgg cttcatcccg ccgccgcatc tcccggccca gacggccgct
      121 cggatggccg gagcggccgg cctgattttt gtgggaaccg cgctgccgga agagatactg
      181 ttccgctcgc tcgtccagaa ctggatgatg ctgcggtttg gcgacaacat tcgcacactt
      241 ctggccgcca gtttcctctt cggctgtgcg catctcgata acggcccgca gcctctgccg
      301 aattggcgct acatgatcct ggcgaccatt gcaggtatcg cctacggcaa ggtctttcaa
      361 agatcctcga cggtcctttc ctccagcggc ttgcacacgc tggtgaattg gacgaaacat
      421 tctttctttt gagtgaccgt gtatcgcaag ctcgccattc tggaaccact cgctgtcttc
      481 ggcctgatcg ttgcgtacat ctggggactc cgcgctatgt accccaacct ctggatagcg
      541 atcctggggt tgatgctgct ctcgcacctg ctccatggcg aaagccccgg actgctgggc
      601 ttcgggacgc gcaacctgca aggtgtaatg cgcgagattg caccgccgct tgcgtttctg
      661 gcgcttctga tgctggcttg cggcattctg ctccgcacca tccgaccgat cagtgttgac
      721 ggagccgtcg tggcactggc cggctatctg ccgtggggcc tgttgcagca gtacattttg
      781 aacggctact ttctgaaccg cttggacgca gttctctccc cacgcgcggc ggtagttatc
      841 acggcgatgc tcttctgcgc cgcccatgca ccgaactggt tcttgatggg cgtcacgttg
      901 ccgggtagtt attgggctac acgcgtctat cagcggcgcc gaaacctcta tgtcatgggc
      961 ctggctcacg ccatcgtagg attcctgctc ttcctggtgg ttccggattc gattactcat
     1021 cacctccgcg tcgggccggc gtggtttagc cgataagttg cgacttgcgg ggaggacgcc
     1081 gagggagaaa cacacgccag acggccatca acggtcaggc attaaaagca ccgcagtgct
     1141 ccgtacgagg ccgaaaagca tcattgccca ggccgcaacc cggcgacggg ctgtttcgcg
     1201 gttcacgtgc acatctgcta tgaagcgata gccgcggcga ggcactgtct caatgaatcg
     1261 aggattccga ggcgagtcgc acaaagcgct gcgggctttc ttgatcgccg cgtttagccc
     1321 ttggtcgaac tcgatgaagg taccgtcggg ccagagcgcc ttttcgagtt cctttcgggt
     1381 cactacttct ccaggccgtt gaaccaacag agcaagcgcc tggaagggtt gctgctggag
     1441 cctgactcta cgccccttct tacgaagttc gcccgtggcc aggtccgcct cgaatagtcc
     1501 gaagtgcacc gagattgccc ctttagcgct gctcaacatc gctaattgcc cagttttgtg
     1561 aactccctgt tacgggcgag acatagatgt agaatgtcag acagaaaatc cccgtcctac
     1621 agcgggccca gcgcggtaat caaaatctgt aacggcatcg agtactggcc ttcgtgcgtc
     1681 ccgttgtgca gggtggcgcg ctcgtacaac gacgggtaca cggtgtcagc cagctcgcca
     1741 tccacaatcc attgcgccgt gttgctcagt gaccagggct gcgaagggtt cggcggaggc
     1801 atgtagtgca ctctcaccca cgaggtctcc accggcagat tgctgtcggc caggatgccg
     1861 ccgtgtatgt tgggaacgca caccaagtct cccgggcacg cccaggcatc cggcaggagg
     1921 cgcagatgat gggtcttacc atccggcgcc ggaaactggc tggccatgct ggtgtaaaaa
     1981 tacgggtcct tggagccgag gaggtttcgg acgttgaaga acgcctggct ttggaagggt
     2041 gcgcctcccg caaacgacgg cggcgggttc acgatattcg agttcggaaa cggattgggg
     2101 acccggatcc aaacggagcg cttggagtgg gcgagcacca gcgtggcgtc gttggtgggg
     2161 ttactgccgc agcggtggat gcttccattc tcgtacggtt ttgttgggtc atcattccat
     2221 atcgccgccg gagccgccgt ggtgctcaag aacgtgtagc tgatcgggag atcctggcag
     2281 ccagtcctgg gggctgccga gctgcgggtt ggcagcggcg ccaacaacgt agcagccatt
     2341 gccgggatga cgagaagaga tgctttgcca aatcgtgtca tgtgtcctcc ttgaaagcca
     2401 gattgattcc cgaaaaagca ccggctgctg ccggccttcg ccttttgcat ccggcccctc
     2461 gtttcgggga tgacctgagt gtagtcggat cgccagcgga gagtcatcaa gattctgcca
     2521 agatttcgtg cgattctcgc ggggaaacaa gttcgggttc tcagccgaac ctcaaagggc
     2581 attttcagcc gttttcagga ccgcgcgagc caaactggct gcgccctccg tgcgtccgcg
     2641 gtgatccgcc ccgaaactta acagaaactt gacagaaggg tgtggacagg tagtgttagg
     2701 atctctctaa ctgggggtag gatgtccatt ccgcgctgtt ttcctgcctt tgccctgtta
     2761 gcgggcgttg tcgcgagcgc accgtcgttg tgcgcgcagt ccactttcgg cagcatcgtc
     2821 ggttcggtga aggacctctc cagcgccgtc gttccagcag ccacgatcgc cgctcgtgac
     2881 gttgaagaga acacaaccag gactacaacc tcaaacgatc aagggttgta cgaactgctc
     2941 aatctcaagc ctggcagata tgagatcacc gccacgaagg ccgggttttc gacgttcacg
     3001 atccgtgcgg tgatgctcct ggcgcgggac acggttcggg cggatgtccg gctggaggtg
     3061 tcgcccatca aggaatcggt tgctgtaacc ggcgaggcgt caatcatcaa taccgagaat
     3121 gggaccatct ccgacacgaa gagcttccgt gagatcaccg ggttaccgct caactaccgc
     3181 ggcgttacca ccagccctct gatagccctc ataagcgtgc ccggcgtaca gatgtccctc
     3241 ctcgatcgcc cttctatcgg gggcgggctg ccggcgcaaa tcgaatacag tctcgacggc
     3301 atatcgacgg taaacatcgc attttttgga ccgatctatg acatgtaccc ctctacggag
     3361 atgatcagcg agttcaaagt gatctccgtg aacaatagcg cggagtacgg gcaaatgggt
     3421 gatgtgaccg tcatcacgcg cggcggaacg aaccagttgc atgggagcgc ggtgtggtat
     3481 caccagaaca gcgccctgga cgccacgatc tacggtgcgc ccgaaaagca gtccaaggtc
     3541 ttcaacacgt ttggcggcag cttgagcggc ccactgtacc tgccgggcct ctatcacggg
     3601 cgcgatcgca ctttcttctt tgttgactac gaagggaacc gcaagccgtc tgccgtggtg
     3661 gaacatctat cggttcccac agcttcgatg cgtgccggag atctgaatgg cgtccccggc
     3721 cagtccgcgt cggatccgct ttccggggct ccgtttccga acaacgcaat ccccgcaacg
     3781 cggattaatt ctgtggcccg cacgctgttc cagaggtact atccgctgcc caacttcaac
     3841 accgatggca caacgttcga caattaccgc tcggtggtcc cgacctcgat ttacacggac
     3901 gggtatgacg tgcgcgtcga tcacgtgctg aactcccggc atcagatatt cgggcgctgg
     3961 agctggaagg agatctccgg taaaggggcc tacgaattgt tgccccccga acagtacgac
     4021 tccgcaaatc ggaacctgat cctgtcccac aatttcgtgt tgcgccccaa tgtattcaac
     4081 gaatcccgat tcggcttcgg cctgtctgac ggccgcacgc gattcccgat tctgggcgct
     4141 gacgcggtgg ccacgctggg tcttgagggt ctgaacctgt cgaacgctgg aggtgtgggc
     4201 ggcttcccgc tgttcgattt ctccgaaggg accggcttct acctcatcgg ccacccgcgc
     4261 gatggtccag gccgctcgcg aaactggcag ttcaccgaca acttgagttg gatccgcggc
     4321 cgccacagca tgaaattcgg cgccgacatc cggcgcatcg gctgggtagg ggtgttgagc
     4381 gatggcgggg gtgacgactt cgggagtttc cttttccagc cggacgcctt cagcggaaat
     4441 gcctttgtgg acctgctgct gggcctgccc tacctaaccg aatacgggtt gcttggaccg
     4501 aacctggatg aatcggcaac gcacgctcac ttttacggcc aggatcagtg gcaggtaaat
     4561 gaccgtctca cggtcagcat cgggattcgc tggagcgtcc atccgcctat gaccgaagcc
     4621 tccggcaaca taaccaattt cgacccggcc agcggaaacg tcatcgtgcc ggatcacacg
     4681 attccagccg ctcccggctt cctcaccgga attaatgcat gccccggtgt gacaaccgct
     4741 ttcccttgca cgaagatcgt cacggcgagt gaggcgggcg tggggcaggg cctacgtcga
     4801 acatactatg gaaactgggc gccgcgcgtc agcattgcat ggcgtccctt cgcgagtaac
     4861 aagacagttg tgcgctccgg gtttggcgag ttcacgcaga cgattctggg agcaacggcg
     4921 tccggactaa ccgccatcca cagtaccgac ctgcgggacg agtttaatta ccagggtccc
     4981 ggagtggccc cacagtttgt tctgccgctg gttgccagtg gcgcttttgt tctgcctcag
     5041 cccggtacca agacttttgg agacgggatg gatacgtcat acaaagaccc tcgatcgtat
     5101 caatggaact tcacgctgga gcatgctctg gccgcggaga ccaggttgcg gctcagttat
     5161 atcgggagcc actccgtagg tctcaacggc ttggtgaact acaaccagca gcatgccagc
     5221 acaacgccgt tttcggcaag ccgcaggccg taccccgcgt tcaggactgt ggggatgtgg
     5281 gaaaacatcg gctttgccag ctacgaagcc tttcaggcgg aggcgactcg aaggtttgcg
     5341 cgtggcctgt tcttccaggc aagttacatc ttctcgaaaa atatcggcaa cgccggtagt
     5401 gcctatggcg gccagggcgg tctcctcttc gtgcctgagg cgctgccaaa cgctgtcacc
     5461 gatcgtttca atacccgcct cgaccgtggc attcttgcgg gatcgcgtgc gaaccggttt
     5521 ctgttcacgg gcatctacga gcttccgtgg ggcaaggggc ggcgatatgc gaaccggctg
     5581 ccggcgcttc cgaacgctct tttcggcggg tggaacctga gcacgatcac gttgctggag
     5641 tccgggccgt tccagacggc cttcacacga gcgcctgacc catccaattc aggagcaggc
     5701 gggcgactca aacggccgga ccgcatcggt gatggaaacc ttccccatcc gactccggac
     5761 cactggtacg acatcaccgc attcgtgcct accccccagg gagca
//
LOCUS       DATIFQ010000164         5328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2354074, whole genome shotgun sequence.
ACCESSION   DATIFQ010000164 DATIFQ010000000
VERSION     DATIFQ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354074"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VLE22_07440"
     CDS             <1..1129
                     /locus_tag="VLE22_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HSB14277.1"
                     /translation="LAAAPVHGALTRDTIERAAGAAGFRVKAVQMNVPRAVRPRLPFV
                     PFDFDDPLLEGLRREYRLSEVAGDSRDEWTRQLKLKDWIFRQIPGGNPRHSPRNALEV
                     LRLAAKGEKFYCTQYAITYVECAQALGWPARKIGVDRRHGPKGLGSSHHGVAEVWSNQ
                     FQKWVVMDAQSNLHFEKKGVPLSAWELRSEWLKNKGADVDHVVGCPPDAAKKNPAMVW
                     KVPDEDEIATYFWLYIEDHANTGGQAGRQILPLDEDNLDEIWYQNDYEAGESRIHVGY
                     TSGRFLPTKRLDDAYWTVGIVEAEVSGAEPGVIQMRLNSYCPNQTGYEICRDGRTWER
                     VTGEGAVEWKLKEGWNTFRARTVSRGEVRGPEAAVVMALEK"
     gene            1133..1864
                     /locus_tag="VLE22_07445"
     CDS             1133..1864
                     /locus_tag="VLE22_07445"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HSB14278.1"
                     /translation="MSGSHVFLQYVRRSFVLRWALLFAAFGIPLAVLAVSEGIRTHRR
                     VQDFYPTRAEIQAKYRIGGRGGPSHFLLIRYLDRAGGQHERRVGASRGEYAEARRGDE
                     IAAYVSGIDPSDAWLVSAGQPNYKRTEALAGVAGGMFVPLVAMLEWLRRRAAVLRDGH
                     PVTGRVEKSGRDYRIRFSTKFHYRVTWSCTGPDGRLRRGKSLHVPKPFASKWRPGDEI
                     EVYFDPRHPWLAEVDVYGMRNGDRK"
     gene            complement(1887..3197)
                     /locus_tag="VLE22_07450"
     CDS             complement(1887..3197)
                     /locus_tag="VLE22_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14279.1"
                     /translation="MNRRHFVMSSAAAAGTLATRGLASRNDTVRLACVGVRGQGRAHI
                     QNYAKMANVEIAALCDVDQSVLNQRLQDLDKLGKKRPAVFTDYRKLLEDKSIDAVSIA
                     TPNHWHTLQTIWACQAGKDVYCEKPCSHNMFESRQIVEAARKYNRIVQHGTNSRSGVA
                     IREAMEHIRTGLIGDVYMARGVVFKWRDTIGRKPVQPVPAGVDYDLWLGPAPKREFTP
                     NRFHYTWHWFWDYGNGDFGNQGIHELDVARWGLGVTYPTRISAMGGHFMFDDDQETPN
                     TLVVNFEFNSGGKKKLLVFEVRHWISNRESRICESRDIRKDPNTVGNLFYGSKGYIGI
                     EWYEKYYSMLGKEQQPGPSREEDGNNWENFIQALRSRKLSDRNAPIEEGAISCALMHL
                     GNISYRLGRQLNFDAATMTCVGDDEANRMFKRNYRAPFVVPEKV"
     gene            3266..4456
                     /locus_tag="VLE22_07455"
     CDS             3266..4456
                     /locus_tag="VLE22_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB14280.1"
                     /translation="MASLLPLAAQTRLTAQAVQISVRFKISDVRVVSLRTVKEAGSIE
                     PAWNPGGTMSFRVGGGSFTEIVTDQGLAGIGPGIDPGLLPAVKAQLVGKDPFDIEVHS
                     ARLRYYAGGSPYRGSGAVDIALWDLIGRATEQPLYKLWGGGKDKVPAYASLIRLSTPE
                     ERARLAAQLMSEGWKAIKLRLHHETMKEDLRTVEAVRAAVGDRMAILTDANQAQSSGN
                     WQPGVLWDFRRAVESARELQRMGCYWLEEPLPRYAFDQLAELNRLVEIPLAGGENNRG
                     VHEFLTMLREGVYDILQPESLVTEGVTGLRKIGVLAEAYGKRVVPHHGGGNLGTIAHL
                     HLVASWAHAPYLELLHDPPVGDYRHGLSILRDPPLVDKEGFVHLPKGPGLGVEINPDL
                     IAKG"
     gene            4462..>5328
                     /locus_tag="VLE22_07460"
     CDS             4462..>5328
                     /locus_tag="VLE22_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14281.1"
                     /translation="MNLIVVLALLLQAAHSLKAVPLDASDAADTFRFEASPEKVQSGS
                     WRVAGLPRKYFDELLPALFERTLAIERFEPAQGALEWIFTGPSGGFTIRVEDGALRVT
                     QRYYDSFGLRDESRRTRHPERITEESTVPWQGRLQGITVRLDHRLGLSVLLNGRETVR
                     QNCVLDVSRHQLGWSGSTGVSAGSLLEPEAASAIVRVDAKARMQTMIGFGGIAIPTAY
                     AQLSPAGKRRWWELLAEYNLLLHREYPIGAKLNERMDNWDRIEDATPHYYGDNFPNGE
                     ISNFEYIRNVRRI"
BASE COUNT          987 a         1492 c         1892 g          957 t
ORIGIN      
        1 gttagccgcc gcgccggtac acggggcgtt gactcgtgac acgatcgagc gggcggccgg
       61 agcggccgga ttccgggtga aggccgtcca gatgaacgtg cccagggcgg tgcgccccag
      121 actgcccttc gtcccgttcg acttcgacga tccgctgctc gagggattgc ggcgggagta
      181 ccggctgagc gaggtggcgg gcgattcccg cgacgagtgg actcggcagt tgaagctgaa
      241 ggactggatc ttccggcaga tccccggagg caacccgcgg catagcccgc gcaacgcact
      301 ggaggtgctc cggctggcgg cgaagggcga gaaattttat tgcacgcagt atgcaatcac
      361 gtacgtggag tgcgcccagg cgctcggatg gcccgcgcgc aagatcggag tggaccgcag
      421 gcacgggccg aagggcctcg gaagcagcca ccatggcgtg gcggaggtct ggtcgaacca
      481 gttccagaag tgggtggtga tggatgcaca aagcaacctg cacttcgaga agaagggcgt
      541 cccgctgtca gcgtgggagc tgcggagcga gtggctcaag aacaaaggcg ccgacgtgga
      601 ccacgtggtc ggctgtccgc cggacgcggc gaagaagaat ccggcgatgg tgtggaaggt
      661 gccggacgaa gacgagatcg ccacgtactt ttggctctac attgaggacc acgcgaacac
      721 cggtgggcag gcagggcgcc agatccttcc gctggacgag gacaatcttg acgagatctg
      781 gtaccagaac gattacgaag caggcgagag ccggatccac gtgggttaca ccagcggccg
      841 gtttctaccg accaagcggc tggacgatgc gtactggaca gtgggaatcg tagaggcgga
      901 ggtgtcgggc gcggagccgg gcgtcattca gatgcggctg aacagttact gtccgaacca
      961 gaccgggtat gaaatttgcc gggacggacg gacgtgggag cgggtgacgg gcgaaggcgc
     1021 cgtcgagtgg aagctgaagg aaggatggaa cacgttccgc gcgcggacgg tgagccgcgg
     1081 ggaggtgcgc ggaccggagg cggcggtggt gatggcgctg gagaagtgag cggtgtccgg
     1141 atcacacgtg tttcttcaat acgtgcgaag gagcttcgtc ctccggtggg cgctgttgtt
     1201 cgctgcattc ggcatccccc tagctgtgct cgccgtatcc gaagggattc gcacgcatcg
     1261 ccgggtgcag gacttctatc cgacgcgggc tgaaatccag gcgaaataca ggataggagg
     1321 gcgaggcgga ccgtctcatt tcctgctcat tcgatacctg gaccgggccg gcgggcaaca
     1381 cgagcgccgt gtgggggcgt cacgtggcga gtacgcggaa gcgcgaaggg gagacgaaat
     1441 tgcggcatac gtttcaggaa tcgacccgag tgacgcgtgg ttggtcagcg ccggccagcc
     1501 gaactacaag cgaactgaag cgctggccgg cgttgcgggt ggcatgttcg ttccattagt
     1561 tgcgatgctc gagtggcttc ggcggcgagc agcggtccta cgcgacgggc atccggtgac
     1621 aggccgcgtc gaaaagtccg gcagggacta ccgcatccgc ttctcaacga agttccacta
     1681 tcgggtgacc tggagctgca cagggcctga cggccggcta cgaaggggca agtcattgca
     1741 cgtgcccaaa ccgttcgcca gcaagtggag acctggggat gagatcgagg tttacttcga
     1801 ccctcgtcat ccgtggctcg cagaggtgga tgtgtacggc atgcgcaacg gtgatcgcaa
     1861 gtgaaggtag acgtcccggt gcttggtcag accttttcgg gaacaacgaa aggcgcgcgg
     1921 tagttgcgct tgaacatgcg gttggcttcg tcgtcgccga cgcaggtcat ggtagcggcg
     1981 tcgaagttca actggcgacc gagccggtac gagatgtttc cgaggtgcat cagcgcgcat
     2041 gagatggcgc cttcctcgat cggcgcattg cggtcagaga gcttccggct gcggagggcc
     2101 tggatgaagt tctcccagtt gtttccgtct tcctccctcg atggaccggg ttgctgctcc
     2161 ttgccgagca tgctgtagta cttttcgtac cattcgatgc cgatgtagcc cttcgacccg
     2221 tagaagaggt tgccgacggt gttcgggtcc ttgcggatgt ccctgctctc gcagatgcgg
     2281 ctctcgcggt tgctgatcca gtggcggact tcgaagacga ggagcttttt cttgccgccg
     2341 gagttgaatt cgaaattgac gacgagagtg ttgggcgtct cctggtcgtc gtcgaacatg
     2401 aaatgcccgc ccatggcgga aatccgcgtc ggataggtga cgcccagacc ccaccgggcg
     2461 acatcgagct cgtggatgcc ctggttgccg aagtcgccgt tgccgtagtc ccagaaccag
     2521 tgccaggtgt agtggaagcg attcggagtg aactcgcgct tgggcgcggg gccgagccag
     2581 aggtcgtagt cgacgccggc gggaacgggc tgcacgggct tgcggccgat ggtgtcgcgc
     2641 catttgaaga cgacgccgcg ggccatgtag acgtcgccga tgaggcctgt gcggatgtgc
     2701 tccatcgctt cgcggatggc gacgccggag cggctgttgg ttccgtgctg gacgatacgg
     2761 ttgtacttgc gggcagcctc gacgatctgg cgagactcga acatgttatg cgagcacggc
     2821 ttctcacaat agacgtcctt gccggcctgg caggcccaga tggtctgaag cgtgtgccag
     2881 tggttggggg tggcgatgga gacggcgtcg atggatttgt cttcgaggag cttgcggtaa
     2941 tcggtgaaga ccgcggggcg cttcttgccg agcttgtcga ggtcctggag ccgctgattg
     3001 aggacggact ggtcgacgtc gcagagggcg gcgatttcga cattcgccat cttggcgtag
     3061 ttctggatgt gcgctctgcc ctgcccgcgg acgccgacac aagcgaggcg gaccgtgtcg
     3121 ttccggctgg ccaaccctct tgtggccagt gttcccgccg cggccgcgga gctcatgacg
     3181 aagtggcgcc ggttcatcaa aatcacctcc cgaaggaact atgatacctt ctctcaccgg
     3241 aggcgcagtg aatcgaagac agtttttggc ttccctactt ccgcttgccg cgcagacgag
     3301 gctcacggcg caggcggtac agatttcggt gaggttcaag atctcggacg ttcgtgtggt
     3361 ttcgctgcgg acggtgaaag aagcgggatc gatcgagccg gcctggaacc cggggggcac
     3421 gatgagcttc cgcgtcggcg gcggttcgtt caccgagatc gtcacggacc aggggctagc
     3481 gggcatcggg ccggggatcg atccggggct gctgccggcg gtcaaggcgc agcttgtggg
     3541 aaaggatccg tttgacatcg aggtccactc ggcgcgattg cgctactacg cgggaggatc
     3601 gccgtaccga ggcagcggcg ccgtggacat cgcactctgg gacctgatcg ggcgggccac
     3661 cgagcagccg ctctacaagc tctggggcgg cggcaaggac aaagtgccgg cctatgcgag
     3721 cctgatccgg ctttcgacgc cggaagagcg ggcgcggctg gcggcgcagt tgatgagtga
     3781 agggtggaaa gcgatcaagc tgcggctgca tcacgaaacg atgaaggagg accttcgcac
     3841 ggtggaggcg gtgcgcgccg cggtcggtga ccggatggcg attctgaccg acgccaacca
     3901 ggcgcaatct tcggggaact ggcagccggg cgtgctgtgg gatttccgtc gcgccgtcga
     3961 gagcgcacgt gagttgcagc ggatgggctg ctactggctc gaggagccgt tgccgcgata
     4021 tgccttcgat caactcgcgg aattgaaccg gctggtcgag atcccactgg ccggagggga
     4081 gaacaaccgg ggcgtacacg agtttctcac gatgctgcgc gagggcgtct atgacattct
     4141 ccaaccggag agtctcgtca ccgagggcgt caccggtctg cggaagatcg gcgtactggc
     4201 ggaggcgtac ggaaaacgcg tcgtgccgca tcatggcggg ggcaacctcg gcacgatcgc
     4261 gcatctgcac ctggtcgctt cgtgggcgca cgcgccgtat ctcgaactac tacacgaccc
     4321 gcctgtcggg gattaccggc acggtctatc gatcctgcgc gatccaccgc tcgtcgacaa
     4381 agagggcttc gtacacctgc cgaaaggccc ggggcttggc gtcgagatca atccggacct
     4441 gattgcgaaa ggatagcctg catgaatctg attgttgtcc ttgcgctgct gctgcaggcg
     4501 gcgcacagcc tgaaggctgt gccactcgac gcctcagatg cggcggatac gtttcgcttc
     4561 gaggcttcgc cggagaaggt tcagagcggg agctggcggg ttgctgggct gccaaggaag
     4621 tacttcgacg aactgctccc ggccctgttc gaacgcacgc tggcgatcga gcgattcgag
     4681 ccggcgcaag gcgcgctgga gtggatcttc accgggccgt ctggcgggtt cacgatccgc
     4741 gtcgaggacg gcgcgctccg tgtcacgcag cgctactacg attcgttcgg actgagggac
     4801 gaatcccgga ggacccgcca tccggagcga atcacggaag aaagcacagt cccctggcag
     4861 ggccggctgc aagggattac cgtgcgactg gaccacaggc tcgggctctc ggtgctgtta
     4921 aacggacggg agacggttcg ccagaactgc gtactggacg tcagccggca tcagcttgga
     4981 tggagcggca gcacaggcgt atcggccgga tcgttgctag agccggaggc agcgagcgcg
     5041 atcgtgcgtg tcgatgcgaa ggcgcgaatg cagacgatga tcggtttcgg cgggatcgcg
     5101 attccgacgg cctacgcgca actcagtccc gcggggaaac gccgctggtg ggagctactc
     5161 gcggagtaca atctgctgct ccaccgcgag tatccgatcg gcgcgaagct gaacgagcgg
     5221 atggacaact gggaccggat cgaggacgcg acaccgcact actacggcga caactttcca
     5281 aacggggaga tcagcaactt cgaatacatt cgcaacgtcc ggcggatc
//
LOCUS       DATIFQ010000165         2480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_631502, whole genome shotgun sequence.
ACCESSION   DATIFQ010000165 DATIFQ010000000
VERSION     DATIFQ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631502"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="VLE22_07465"
     CDS             complement(<1..1324)
                     /locus_tag="VLE22_07465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSB14282.1"
                     /translation="MVPESALQQFRTSLRGQLFTPGEPGYEEARKIHNALIDRRPAVI
                     ARCAGVADVISAVHFGRSQSVVVSVRGAGHNVAGTSLCDGGMVIDLSAMKGIHVNPAA
                     RTARVEPGVTWSELNHDLGAFGLAATGGFVGTTGVAGLTVGGGLGWMVRKHGLALDNL
                     LSADVVTADGQLVITDSAQHPDLFWGIRGGGGNFGIVTSFEFQVHPAGTVLAGLAMHP
                     ASKGMEALRFWREYESTAPEEMTNGALVFNAPPELPLPDVLRREPIVGIGGVYTGALE
                     EGERALQPLRSFGPPLVDTYQRMPYSAAQTMADFLWPRGIYNYWKSSYLKSLSDGAIE
                     TVLEFCAKAPSRRSVVVLEHNGDGAMSRVPEDATAFGHRNWPYNFLVTAAWTDPSDTD
                     INIRWAREFWTAMQPFLADAVYVNYLGEVEEERVRAAYGRKYDRLASLK"
     gene            complement(1407..1673)
                     /locus_tag="VLE22_07470"
     CDS             complement(1407..1673)
                     /locus_tag="VLE22_07470"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HSB14283.1"
                     /translation="MCRSIRTLFNFDPPATDEEIRAASLQFVRKISGFNKPSKANEDS
                     FRAAVDEIAGIASRLLVSLETNAVPKVREVEAAKAKARAGRRLR"
     gene            1748..>2480
                     /locus_tag="VLE22_07475"
     CDS             1748..>2480
                     /locus_tag="VLE22_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB14284.1"
                     /translation="MRSHERTRGLLRAPKLAWDVLVRGRYDFTYDQMPMTASRMAWAK
                     RFNLFKAGANLLHQRLQPWSMPLHMQFELTNYCSLRCPVCPTGSKALDRRPQPIDVGL
                     FERVMDEVGPHLLTASLWAWGEPLLHPRLKEILRAARKHDVITLLSTNGQSLNQDRIL
                     DALIQEPPDYLIVAFDGLTDGTNSQYRVGATLAPIRDGVRKLAELKRLRGLSTPLLNM
                     RDIVMNHNQHEVPQLEEFARLHDFEL"
BASE COUNT          487 a          798 c          726 g          469 t
ORIGIN      
        1 ctttcaagga agccagccgg tcatatttcc ggccgtaggc tgcccgaacg cgttcctcct
       61 cgacctcgcc gaggtagttg acgtacaccg cgtccgcgag gaaaggttgc atcgccgtcc
      121 aaaattcacg ggcccagcgg atgttgatat cggtgtctga gggatcggtc caggcggccg
      181 tgaccaggaa gttgtacggc cagttgcgat ggccgaatgc ggttgcgtct tcggggacgc
      241 ggctcatggc tccatcgccg ttgtgctcca gcacgacaac gctccggcgt gacggcgcct
      301 tggcacagaa ctcgaggaca gtctcgatcg cgccgtcact gagcgacttc aggtagctgg
      361 atttccagta gttgtatatg cccctcggcc agaggaaatc ggccatcgtc tgggccgcgc
      421 tgtacggcat tcgctggtat gtgtcgacaa gcggtggacc gaacgagcga aggggctgta
      481 gcgcccgctc accttcctcc aatgctcccg tgtagacacc gccgataccg acaatcggct
      541 cacgacgcaa cacgtcgggc agcggcagtt ccggcggagc gttaaacacc agagcgccgt
      601 tggtcatctc ttccggcgcc gtggattcat actcgcgcca gaaccgcaac gcttccattc
      661 ctttggatgc agggtgcatg gcaagcccgg ccaacaccgt cccggcgggg tgcacctgga
      721 attcaaagga agtgacgatg ccgaagttcc ccccgccacc ccgaatcccc cagaacaagt
      781 ccgggtgctg ggctgaatcg gtgatgacga gttggccatc ggcggtgacg acatccgccg
      841 acagcaggtt gtcgagcgca aggccatgct ttcgtaccat ccaacccagt ccgccgccca
      901 ccgtgaggcc cgcgacgccg gtagtcccga cgaaaccgcc ggtggcagcg aggccgaaag
      961 cacccaaatc gtgattgagt tcgctccacg tgacgcccgg ctccacgcga gctgtgcgcg
     1021 ccgcggggtt cacgtgaatc cctttcatcg cggaaaggtc gatcaccatg ccgccgtcgc
     1081 acaggctggt gcccgctaca ttgtggcctg cccctcgaac tgaaactacg acgctctggc
     1141 tccgtccgaa atggacggcc gaaatcacgt cggccacgcc ggcacaccgt gcaatcaccg
     1201 ccgggcgccg gtcaatcaac gcattgtgga ttttgcgggc ctcctcatag cctggctcgc
     1261 cgggagtaaa cagttggccc cgcagcgacg tgcggaactg ttgcagagca gattccggaa
     1321 ccataagccc agcccccttg ttcaatagac taccggcgaa ccgcagttaa agtcaaagcg
     1381 caccgcccga agaccgcccg tcaggctcag cgcaatcgcc gcccggcgcg agctttcgcc
     1441 tttgcggcct cgacctctcg gactttcggc actgcgttcg tttcgagcga aacgagaaga
     1501 cgacttgcga tgccggctat ttcatccacc gcggcccgga acgaatcttc gtttgccttc
     1561 gacggcttgt tgaatccgct gatctttctc acgaactgga gcgaagcggc tcgaatctct
     1621 tcgtcagtgg ccggcggatc gaagttgaag agggttctaa tactcctgca cattccgtat
     1681 gacctcaaag gtatcagatc tcttaagggc tgctccggga tgcgataatg gacctcccga
     1741 cgatggcatg agatcgcatg aacgcactag gggtctgctg cgggctccca agctggcttg
     1801 ggatgtgctc gttcggggac gctacgactt tacatacgat caaatgccca tgaccgccag
     1861 ccggatggcc tgggccaaac ggttcaacct ctttaaggcg ggcgccaatc tcctccacca
     1921 gcggttacag ccttggagca tgccgctgca catgcagttc gagctgacca actattgcag
     1981 cctgcgatgc ccggtgtgcc cgacaggctc caaggcgctg gaccgacggc cccagcccat
     2041 cgacgtgggg ttattcgagc gcgtgatgga cgaagtgggt cctcacctgt tgaccgcgtc
     2101 gttgtgggcc tggggagagc ctttgctcca cccccgcctc aaggaaatcc tccgcgcggc
     2161 tcggaagcac gacgtcatca ctctgctctc gaccaacgga cagtccttga accaggaccg
     2221 cattctcgac gctttgatcc aggagccgcc cgactacctc atcgtggcgt tcgacggact
     2281 gacggatgga acgaactctc aataccgggt gggcgcgacg ctcgcgccca tccgggatgg
     2341 cgtcaggaaa ctcgccgaac tgaaacgcct caggggcctt agcacccctt tgttgaacat
     2401 gcgcgacatc gtgatgaatc acaaccagca cgaggttccg caactggagg agtttgcccg
     2461 cctgcatgat ttcgaactgc
//
LOCUS       DATIFQ010000166         6547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2162960, whole genome shotgun sequence.
ACCESSION   DATIFQ010000166 DATIFQ010000000
VERSION     DATIFQ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162960"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VLE22_07480"
     CDS             <1..507
                     /locus_tag="VLE22_07480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB14285.1"
                     /translation="DCFTFEGGFALHLAGGCESVEAVDSSEHALCLARENAGLNEIGN
                     VEFRQADVFDLLAGYSAAGRRFDTVVLDPPGFAKSRSAVEGAARGYKDLNLRALRLLG
                     AGGVLVSCSCSHHVSEAMLLEIVASASLDAGRKLRVLERRTQALDHPILLTVPETHYL
                     KCVILEVA"
     gene            686..3199
                     /locus_tag="VLE22_07485"
     CDS             686..3199
                     /locus_tag="VLE22_07485"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HSB14286.1"
                     /translation="MFRSEALPVIQRQASSQKASGRPAILGLLRAVLVLLSLAVLAAP
                     MFAQEHHGGEANLILPDLDQATFLGGIGGRSLLMVGLLVSGLGFLFGLVIYKRLRDMP
                     VHAAMREVSELIYETCKTYLATQGRFLLILEALIAVIIVFYFGFLQGFEAFRVAIILV
                     FSVVGISGSFLVAAFGMRVNTFANSRTAFASLRGKPFPCYDIPLQAGMSIGMLLISVE
                     LLIMLFILLFVPGNLAGPCFIGFAIGESLGAAALRVAGGIFTKIADIGSDLMKIVFKI
                     KEDDARNPGVIADCTGDNAGDSVGPSADGFETYGVTGVALIVFILLAVREPLVQVQLL
                     VWIFSMRVMMVIASAVGYFVNGFIAKARYAAADKMNFETPLTTLVWLTSILSIVLTFI
                     LSAFMIPELVGDTSLWWKLSTVISCGTLAGALIPEFVKVFTSTKSRHVGEIVTASREG
                     GPSLNILSGFVAGNFSAYWLGFAMLLLMGIAYRTSTLGLHGLMVETPMAIEAVFAFGL
                     VAFGFLGMGPVTIAVDSYGPVTDNAQSIYELSTIEELPNIKQDLKKNFGFEPEFEKAK
                     ENLEENDGAGNTFKATSKPVLIGTAVVGATTMIFATVVALTSGLRENVDKLSLLHPPF
                     LLGLITGGAVIYWFTGASVQAVTTGAYRAVEFIKRNIKLEGSTKASVADSKKVVAICT
                     QYAQKGMFNIFLTVFFSTLAFAFLEPFFFVGYLISIALFGLFQAFFMANAGGAWDNAK
                     KVVETELRAKGTDLHAATVVGDTVGDPFKDTSSVALNPIIKFTTLFGLLAVELAVSMT
                     RQQGPMITNVLSAIFLALSMVFVYRSFYSMRIESTGADK"
     gene            3321..4091
                     /locus_tag="VLE22_07490"
     CDS             3321..4091
                     /locus_tag="VLE22_07490"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSB14287.1"
                     /translation="MSAAIDYFERTAKLLEELRRTQLPAIEQAAGICASSIAQGGLIF
                     LFGAGHSRMMCEEMIPRQGCFAGFFALVELAISTHSAIVGVNGLRTALHLEKYEGYAE
                     EILKSFRFGPHDAFIIISTSGIRPVVVEMAMGAKQRGMPVIAIVSKLHSAQARPGHSS
                     GKKLPDFADIVIDNMCPPGDCVVPLAGLDWATGPVSTVTGAMIINMLRCETARLLTER
                     GVKPVMLPSHQVPGNIAADEQLERFYEAYRKSLTHLYE"
     gene            complement(4096..5631)
                     /locus_tag="VLE22_07495"
     CDS             complement(4096..5631)
                     /locus_tag="VLE22_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HSB14288.1"
                     /translation="MNSRRTFLAWTAAAPFLSRLSRPSLAARRFDPSFGTASEALRAI
                     REGVISSRELLEHTLGRIRKYNPGINSFVTMNEEEATRRAREADDSLARGKNPGRLHG
                     LPILVKDTFETAGLRTTAGAKPLENHIPKKDAAAVARLRAAGVLIVGKTNTPEFAGDT
                     QTFNAIAGTTNNPWNVKRTPGGSTGGGAAALAAGFGFLELGSDIGGSIRTPAHFCGVY
                     GHKSSLGIVPLEGHIPPSPGEPALPPDLGVAGPLARSARDLQLELETIAGPSRPDPTA
                     FRWKLPSPRGARLRDYRIGFVLDDPFCPVTPEVREPLRGAVEALRKAGVDFEEGWPAG
                     VNPGEMFGNYLVLLSASFAAHAEEEELETLRRLPPGPWGEYGKAWIEGKTMGHGRWLE
                     ESMKRLRARAVWQKYFETHDAFLMPDMFLPAFPHNQEKTFFERSFDTSLGRRPYGDLL
                     RWISFANLTGCPATVAPVGRTKDGLPVGIQILGPYLEDATPIDIAGRMADVVGGFEPP
                     PGY"
     gene            5711..>6547
                     /locus_tag="VLE22_07500"
     CDS             5711..>6547
                     /locus_tag="VLE22_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14289.1"
                     /translation="MKERPLRTTVIGSYPFPGWLEHASNRLEQFGPADIEEMQEDAVI
                     AAVHDQVAAGLDVITDGEQTRFDFNLSFYGFLEGIEQESSPRRRFGPPAHDQRGHHRI
                     TGPLNAPRGLGAVAEFRRLKRVAPEGPVLKASVPGPYTLSGRLVPGEVYGDRYAVAEA
                     LIPIVRAELEALVAAGCTEITVDEPSMSCYAWREDTSRFVDIFNCTVEPVFGKCRLST
                     HLCFGNYKGRAVGPRRYAPMFPAFLNFKVDEIHLEMASREFAEIEIVAEIARSKDVAV
                     GII"
BASE COUNT         1110 a         2162 c         1930 g         1345 t
ORIGIN      
        1 gactgcttca cgttcgaagg cggtttcgcg ctgcacttgg ccggcgggtg tgaatccgtg
       61 gaggccgtgg acagttcgga acacgcgcta tgcctggcgc gcgagaacgc cggcttgaac
      121 gaaatcggca acgtggagtt ccgccaggcg gatgttttcg acctcctggc cggctactcg
      181 gcggcgggtc gccgcttcga taccgtagtc ctggacccgc cgggtttcgc caagtctcgc
      241 tccgccgtcg aaggcgcagc ccgcggctac aaggacctga atcttcgtgc tttgcgcctg
      301 ctcggcgccg gcggcgtgct cgtcagctgt tcctgctccc accacgtgag cgaagcgatg
      361 ttgttggaga tcgtcgcctc ggcgtccctc gacgccggcc ggaaactccg tgtccttgaa
      421 cgccgtaccc aggccctcga ccacccgatt ctgctgaccg tccccgagac ccattacctc
      481 aagtgcgtta ttctggaggt tgcgtaaacg gctctgcctg ccgctcgtga accaaagcta
      541 agttgattcc gacatggcgg tccgatatag tcaaaagtgg gccgcaaacg taaagaattg
      601 ctctattctc agatctttcg cccgccggcg acgtggccca gctcgccggg ggttccgtgt
      661 actcacaatc aggaggctct tcgtaatgtt tcgcagtgaa gccctgcccg tcatccagcg
      721 gcaggcaagt tcccaaaagg cgtctggccg gcccgcgatt ctcggtctgc tgcgcgcggt
      781 tctcgttctt ctctcacttg ccgtcctcgc ggcgcccatg ttcgcccagg agcatcacgg
      841 cggcgaggcc aacctcattc tgcccgatct cgatcaggcg acgtttctgg gtggaatcgg
      901 cggccggtcc ctgttaatgg tgggactgct ggtcagcggt ctcggcttcc tcttcggcct
      961 tgtcatctac aagcggctgc gcgacatgcc ggtgcacgcg gcgatgcgcg aggtttccga
     1021 gctcatctac gagacgtgca agacctatct cgcaacgcag ggcagattcc tgctgatcct
     1081 cgaggccctg attgccgtca tcatcgtttt ctacttcggt ttcctgcaag gattcgaggc
     1141 gtttcgcgtc gccatcatcc tggttttcag cgtggtcggc atctccggca gcttcctggt
     1201 ggccgccttc ggcatgcgcg tgaacacctt cgcaaattcg cgcaccgcct tcgccagcct
     1261 ccgcgggaag cctttcccgt gctatgacat cccgctccag gctggaatga gcattggcat
     1321 gctgctgatt tcggtcgagc tgctcatcat gctcttcatt ctgctgtttg tgcccggaaa
     1381 cctcgccgga ccttgcttta tcggattcgc catcggtgag tcgctgggcg cggcggcgct
     1441 gcgtgtcgcg ggcggaatct ttaccaagat cgccgacatc ggctccgatc tgatgaagat
     1501 cgtcttcaag atcaaggaag acgacgcgcg caatccgggc gtgatcgccg actgcacggg
     1561 tgacaacgcg ggtgactcgg ttggtccttc ggcggatggc ttcgagacct atggcgtcac
     1621 cggcgtggcg ctcatcgtgt tcatcctgct ggctgtccgc gagcccctcg ttcaggtcca
     1681 gttgctcgtc tggatcttct caatgcgcgt catgatggtc atcgcgtccg cggtgggata
     1741 cttcgtcaac ggcttcatcg ccaaggcacg atacgccgcc gccgacaaga tgaacttcga
     1801 gacgcccctc acgaccctgg tctggctgac ctcgattctg tcgatcgtcc tcaccttcat
     1861 cctcagcgcg ttcatgattc cggagctcgt cggcgacacg tcgctgtggt ggaagctctc
     1921 caccgtcatc tcctgcggca ctcttgccgg cgcgctcatt cctgagttcg tcaaggtgtt
     1981 cacctccacc aagtcccggc acgtcgggga gatcgtcacc gcctcgcgcg agggcggtcc
     2041 gtcgctcaac atcctctcgg gttttgtcgc cgggaacttc tccgcgtact ggctcggctt
     2101 cgccatgctg ctcctgatgg gcatcgccta cagaaccagc acgctcgggc ttcacggcct
     2161 gatggtggaa acgccaatgg cgatcgaagc agtcttcgcc ttcggcctgg ttgcattcgg
     2221 cttcctgggc atgggtccgg ttaccatcgc cgtggacagc tacggtccgg tcaccgacaa
     2281 cgcccagtcg atctacgaac tctccaccat cgaagagctc ccgaacatca agcaggacct
     2341 aaagaagaac ttcggcttcg agcccgagtt cgagaaggcg aaagagaacc tcgaggagaa
     2401 cgacggcgcc ggcaacactt tcaaggcgac ttcgaaaccg gtgctgatcg gcaccgctgt
     2461 ggtcggcgcg accaccatga tcttcgcaac cgttgtcgcc ctgacgagcg gcttgcgtga
     2521 gaacgtggac aagttgtcgc tcctccaccc gccgttcctg ctcggcctca tcaccggcgg
     2581 cgcggtcatc tactggttca ccggcgcgag cgtccaggcg gtcaccaccg gcgcttatcg
     2641 cgcggtcgag tttatcaagc gcaacatcaa gctggaaggc tccaccaagg cgtccgtcgc
     2701 agactcgaag aaggttgtgg ccatctgcac gcagtacgcg cagaagggca tgtttaacat
     2761 cttcctcacc gtgttcttct ccacgctggc tttcgcgttt ctggagccgt ttttctttgt
     2821 cggctacctg atctccatcg cgctcttcgg cctgttccag gcgttcttca tggccaatgc
     2881 cggcggcgcc tgggacaacg caaagaaggt tgtcgaaacc gaactgcgag ccaagggtac
     2941 ggacctgcac gcggcaacgg tcgtcggcga caccgtcggc gatccgttca aagacaccag
     3001 ttcggtggcg ttgaacccga tcatcaagtt cacgacgctg ttcgggttgt tggccgtcga
     3061 actggcagtc tccatgacgc gccagcaggg ccccatgatc accaacgtgc tctccgcgat
     3121 cttcctcgcg ctctcgatgg tattcgtcta ccgcagcttt tatagtatgc ggatcgagag
     3181 cacgggagcc gataagtaac cttcgtccga atcacggcaa aaggggcagg cgttgcctgc
     3241 ccctttcgca tttcttgggt ctggtacgat cgtctcaagc ccgccgtctc gcgtttcggc
     3301 gggcccaccg gagggatttc ttgagcgccg cgatagacta cttcgagcga accgcgaaac
     3361 tgctggagga gctgcgtcgc acgcagttac ccgccatcga gcaagctgcc ggcatctgcg
     3421 cttcgagcat cgcccagggc ggtctcatct tcctgtttgg ggccgggcat tcccgcatga
     3481 tgtgtgagga gatgatcccc cgccagggct gcttcgccgg cttcttcgcg ctcgtcgaac
     3541 tggcaatctc cacccattcg gccatcgtcg gcgtcaacgg gctccggacg gcactccatc
     3601 ttgaaaagta tgaaggctac gccgaagaga tcctgaagag ctttcgcttc ggccctcacg
     3661 acgcgttcat cattatctct accagcggca tccgtcccgt cgttgtggag atggcgatgg
     3721 gagcgaagca gcgcggcatg cccgtgatcg ccatcgtctc caagctgcac tccgcgcagg
     3781 cgcgtcccgg ccactcgtcg ggcaagaagt tgccggactt cgccgacatc gtgatcgaca
     3841 acatgtgccc gccgggcgac tgcgtcgtcc ccctcgctgg gctcgactgg gccacgggcc
     3901 ccgtttcgac ggtcaccggg gctatgatca tcaacatgct gcgttgcgag accgcccgcc
     3961 tgttgaccga acgcggcgtc aagcccgtca tgcttcccag ccatcaggtt cccggcaaca
     4021 tcgccgccga cgagcaactc gagcgtttct atgaggcgta ccgcaagagc ctgacccacc
     4081 tctacgagtg aaacattagt accccggtgg aggctcgaaa ccgcccacga catccgccat
     4141 acggcccgcg atatcgatcg gggttgcgtc ctcgaggtac ggtcccagaa tctggatgcc
     4201 caccgggagt ccgtccttcg tgcggccaac cggcgcgacc gtcgccgggc aacccgtcag
     4261 gttggcgaaa ctgatccagc gaagcaggtc tccgtacggt ctccggccca gcgaagtgtc
     4321 gaatgaccgc tcgaagaaag tcttctcctg gttgtgcggg aaagcgggca gaaacatgtc
     4381 aggcatcagg aacgcatcat gagtctcgaa atacttctgc cagaccgcac gcgcccgcag
     4441 acgtttcatc gactcctcga gccagcggcc atggcccatc gttttgccct cgatccacgc
     4501 tttgccgtac tctccccacg gtcccggcgg caaccgtcgc agcgtctcga gttcttcttc
     4561 ttccgcgtga gctgcgaacg atgccgataa caggacgaga tagttgccga acatctcgcc
     4621 cggattgacg ccggcgggcc agccctcttc aaagtcgact cccgctttcc ggagcgcctc
     4681 caccgcgccc cgcagcggct cccgtacttc cggtgtcaca ggacagaacg ggtcgtccag
     4741 cacaaagccg atccggtaat ccctcagtcg tgcgccccga ggcgatggca gtttccaacg
     4801 aaatgccgtg gggtcaggtc gggacggccc ggcaatcgtc tcgagctcca gttggagatc
     4861 ccgcgcgcta cgcgccagcg gccccgcgac accaaggtcg ggaggaagtg cgggttctcc
     4921 cggagagggc ggaatgtggc cttccaatgg aacgattccc aggctcgact tgtggccgta
     4981 cacaccgcag aagtgggcgg gtgtacggat ggagcctccg atgtcgctcc ccagttcgag
     5041 gaatccgaat cccgccgcca gcgcggccgc cccgccgccg gtggaacctc ccggcgtccg
     5101 cttcacgttc cacgggttgt tcgtcgtgcc ggcgatcgcg ttgaacgtct gcgtgtcgcc
     5161 cgcgaactcc ggagtgtttg tcttgccgac aatcagcact ccagctgcgc gcagtcgcgc
     5221 cacggcggcg gcgtccttct tcgggatgtg gttctccagc ggcttcgcgc ccgccgtcgt
     5281 ccgcaatccg gcggtttcga acgtatcctt gacgaggatc ggcaaaccgt ggagccgccc
     5341 gggattcttc ccgcgtgcca gtgagtcgtc ggcttcccgg gcgcgccgtg ttgcttcttc
     5401 ctcgttcatc gtgacgaacg agttgatccc ggggttgtat tttcggattc gccccagcgt
     5461 gtgctccagc aactcccggg acgagatgac gccctcgcgg atcgccctca gggcctcgga
     5521 cgcggtgccg aagctcgggt cgaaccgccg tgcagccagg ctcggcctcg aaagccggct
     5581 caggaacggc gctgccgctg tccatgccag aaaagtccgt ctgctattca ttcggctttc
     5641 ctcctcgtct cgtactatct tgcgctccgg tggccccgag tatacattgg tcaaaatacc
     5701 gcgtcgacct atgaaagagc gacccctccg aaccactgtg atcggcagct atccgtttcc
     5761 gggatggctt gaacacgcct cgaaccggct cgagcagttc gggcccgccg acatcgagga
     5821 aatgcaggaa gacgcggtca tcgccgccgt ccacgatcag gtggccgccg gcctggacgt
     5881 catcaccgac ggcgaacaaa cgcggttcga cttcaacctc tcgttttacg gttttctgga
     5941 aggcatcgaa caggaatcgt cgccgcgccg ccgcttcggc ccgcccgccc acgatcagcg
     6001 cggacatcac cggatcaccg gtccgctgaa cgctccgcgt ggcctaggag cggttgccga
     6061 gtttcgccgg ctgaagcgag tcgcgccgga aggtccggtg ctcaaggcaa gcgtgcccgg
     6121 cccgtatacg ttgagcggcc gcctcgtccc cggcgaagtt tacggcgacc gttacgctgt
     6181 cgccgaagcg ctgatcccca tcgtgcgcgc cgaactcgaa gccctggttg cggccggctg
     6241 cacggagatc acggtcgacg agccctcgat gagctgctat gcctggcgcg aggacacaag
     6301 ccgcttcgtc gatatcttca attgcactgt ggagccggtc ttcgggaaat gtcgcttatc
     6361 cactcatctt tgcttcggaa actataaggg gagggccgtt ggcccccgcc gctacgcgcc
     6421 gatgtttccc gcctttctca atttcaaggt ggatgagatc caccttgaga tggccagccg
     6481 cgagttcgcc gagatcgaga tcgtcgccga aatcgcccgc tcgaaggacg tggctgtcgg
     6541 catcatc
//
LOCUS       DATIFQ010000167        34679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2547604, whole genome shotgun sequence.
ACCESSION   DATIFQ010000167 DATIFQ010000000
VERSION     DATIFQ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 34679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34679
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2547604"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /gene="sufB"
                     /locus_tag="VLE22_07505"
     CDS             <1..797
                     /gene="sufB"
                     /locus_tag="VLE22_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HSB14290.1"
                     /translation="GQFERTLIVAEEGAYVSYLEGCTAPIRDTNQLHAAVVELVALDN
                     AQIKYSTVQNWYPGDKDGKGGIYNFVTKRGKCQGKNSKISWTQLETGSAITWKYPSCL
                     LIGDGSVGEFYSVAVTNNYQQADTGTKMIHIGKDTKSTIVSKGISAGHGQNSYRGMVK
                     MLKGAEGARNYSQCDSLLIGDQCGAHTFPYLEVKNPTAQVEHEATTSKIGEDQIFYCR
                     QRGIPTEDAVSMIVNGFCKEVFRELPMEFAVEAQKLLSVSLEGSVG"
     gene            806..1600
                     /gene="sufC"
                     /locus_tag="VLE22_07510"
     CDS             806..1600
                     /gene="sufC"
                     /locus_tag="VLE22_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HSB14291.1"
                     /translation="MTKAQRTKTEMLIIRNLRAGIGDKEILKGIDLEVKPGEVHAIMG
                     PNGSGKSTLAQVLAGRDFYEVTGGEVIYEGHDLLELDPEERAAEGIFLAFQYPVEIPG
                     VSNMYFLRAALNNIRKRSGVEELDAVDFLELVKERMTALDMPEGLLNRAVNVGFSGGE
                     KKRNEIFQLAVLEPKLAILDETDSGLDIDALRLVANGVNSLRSPKRSFVVVTHYQRLL
                     NYIVPDFVHVMSNGRIVKSGDKTLALELEQKGYAELDELAVSELVR"
     gene            1597..2922
                     /gene="sufD"
                     /locus_tag="VLE22_07515"
     CDS             1597..2922
                     /gene="sufD"
                     /locus_tag="VLE22_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HSB14292.1"
                     /translation="MIELREDQDVYLTHFADRGQRTAAEPEWLRDLRRSAMDRFAALG
                     FPTTHNEDWKYTNVSGIARLAARPAPAATLTRKELERLPFANLGCGVRLVFLNGRFSP
                     KLSAAKRLPAGVNAASLASVLENGSAPLENHLARYAGFEENAFVALNTALFEDGALIE
                     IAKGTVVEEPIHLVFVSVGGAEPWVAHPRNLILAGRGSQAAVIETYVASGEGVHFTNA
                     VTEIVAAEGAVTEHYKLQHEGAQAFHVGTIQGLQERSSSLTSHNVGFGAALARNDVNV
                     VLDGEGAECMLNGLYATTGKQHVDNHSTLDHAKPNCNSRELYKGILDGQGTGVFNGKI
                     IVHKDAQKTNALQSNKNLLLSADAMINTKPQLEIFADDVRCTHGATVGQLDKDALFYM
                     QTRGIPREAARDLLTYAFAGELFDRMNWAPARERLEQELYRKLSQGRKP"
     gene            2946..4214
                     /locus_tag="VLE22_07520"
     CDS             2946..4214
                     /locus_tag="VLE22_07520"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HSB14293.1"
                     /translation="MKTASETAFPASPEAAGGFNVWRIREDFPILRRQAWDKPLVYLD
                     NAATTQKPQAVIDAITSFYTSDCANIHRGVHFLSERATDAYEESRAKVRRFLNARDTR
                     EIIFVRGATEGINLVARSFVRPMLKEGDEVLITAMEHHANIVPWQMVCGETGARLRVA
                     PIDDRGELIIDEFEKLLGPRTRFVAVSHVSNALGTINPAGMLVEMARSRKIPVLVDGA
                     QAVPHMNVDVQALGCDFYVFSAHKLYGPTGIGALYGRAELLEAMPPYQGGGDMIASVT
                     FEKTVYNQLPWKFEAGTPNIAGTIGLGAAIDYVSAIGLDAIAAHEHGLLAHATGTLSS
                     IPGLKIIGTAHDKAAVISFILDGVHPHDVGTILDREGVAVRVGHHCAQPVMQRFGVPA
                     TSRASFAMYNTSEEVEALTQAVYKVKEIFG"
     gene            4214..4669
                     /locus_tag="VLE22_07525"
     CDS             4214..4669
                     /locus_tag="VLE22_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HSB14294.1"
                     /translation="MSDLRELYQQLIVDHSKSPRNCRAIEGADLSAEGYNPLCGDHVT
                     VYVKLENDRVKDIAFQGNGCAISTASASLLTEVLKGKTRAEAEALFESFHDLVTGHES
                     REKQGAPQLGKLRVFSGVSEFPVRVKCATLVWHTLRAALNQIEEKVSTE"
     gene            4678..5046
                     /locus_tag="VLE22_07530"
     CDS             4678..5046
                     /locus_tag="VLE22_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HSB14295.1"
                     /translation="MPESNEADTDILRQKVIQVLRQIHDPEIPVNIYELGLIYDLSVA
                     ETGEVKIQMTLTAPSCPVAGSLPPEVKSRVEALPGVKEVEVELTWDPPWSMDRMSDAA
                     RLQLGIEPVDDGARVVRFEP"
     gene            5043..5531
                     /locus_tag="VLE22_07535"
     CDS             5043..5531
                     /locus_tag="VLE22_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSB14296.1"
                     /translation="MKLSAHEEYGLRCLLQIGRHGDRGGLTIPQISRLEGLSIHHAAK
                     ILHILKQTGFLKSVRGQTGGYTLAKPPEQVFISDVLSTLGGKLYEEDFCTEHRGRVGN
                     CTHTVDCSIRSLWRMLQTAVDEVLSKTTLRDLIRKEDEMSTWLDQLVTLPTGNEPVTP
                     RA"
     gene            complement(5687..6205)
                     /locus_tag="VLE22_07540"
     CDS             complement(5687..6205)
                     /locus_tag="VLE22_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14297.1"
                     /translation="MMIRCVWVLFLGSCCMMSQPNSTDKLEPLHFLLGEWAGSGQGQP
                     GAGSGGFTFEKDLDGKILIRRGRTEYPATKDRPAFVHTDLTVVFSSPAGLRASYFDNE
                     GHQIQYRVDVGTDQNTITFLSDKEPGRPRFRLIYRKEADQVRVRFEIAPPGQPEAFKT
                     YTEGIVTRSRAR"
     gene            complement(6482..6778)
                     /locus_tag="VLE22_07545"
     CDS             complement(6482..6778)
                     /locus_tag="VLE22_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSB14298.1"
                     /translation="MIRVALPAHLRNMAGVDGEVQLAVKGPLTQLSVLDALEAAFPVL
                     RGTIRDQVTKQRRPFLRFFACGEDLSHEPPDAPLPDAVASGAEPFLIIGAIAGG"
     gene            complement(6775..7890)
                     /locus_tag="VLE22_07550"
     CDS             complement(6775..7890)
                     /locus_tag="VLE22_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14299.1"
                     /translation="MSKVRVLVGTRKGAFILTSDGKRRKWDVSGPHFGGWEIYHIKGS
                     PADPNRLYASQTSGWFGQVVQRSNDGGKTWEPVGNKFVYDGVPGTHQWYDGTPHPWEF
                     KRVWHLEPSLTDPDTVYAGVEDAALFRSADGGQTWQELAGLRGHGSGPNWQPGAGGLG
                     LHTIILDPSDSGRIFIAISAAGAFRSDDGGGTWKPINRGLKSEHIPDPTAEVGHCVHR
                     IAMHRSRPGVLFMQKHWDVMRTDDAGDSWREVSGNLPTDFGFVIDVHAHEPDTIYVVP
                     IKSDSEHYPPEGKLRVYRSRTGGNEWEPLEKGLPQSNCYVNVLRDAMAVDSLDSCGVY
                     FGTTGGQVYASADAGDSWTPIVRDLPAVLSVEVQTLS"
     gene            complement(8093..8917)
                     /locus_tag="VLE22_07555"
     CDS             complement(8093..8917)
                     /locus_tag="VLE22_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14300.1"
                     /translation="MTPKFPARLHVLLASKAPVGVVFRRGPANAVCTVGWNRETGEFK
                     LGQWLRGRIYERRADLSPDGRHLIYFARGGRRHQETKGSWTAISRAPWLRAVTLYGKG
                     DCWQGGGLFTSNTTYWLNGCHFVLRKSAELAEDQEYKPAGFFGAECPSVYYRRLLRDG
                     WTLEMKAGPGVVAPCTVFTRPLRHGWILRKFAYADIDHPEGAGVYWDEHELEHPGRKA
                     LLSVPTWEWADRDGPALVWAEKGILYRALVNPLGPAAAEVLQDFNSMQFESRVAPY"
     gene            complement(8929..10299)
                     /locus_tag="VLE22_07560"
     CDS             complement(8929..10299)
                     /locus_tag="VLE22_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14301.1"
                     /translation="MKTEQSRISVTLTSLVTLAALLEIILILTRRLGDLRGHLVQAIV
                     LFLLSSIVYIVCAYLILRIEKANKSIKYNRLWILIAAGAALFRLTVWPLPPALSDDLF
                     RYRWEGKLQVYGGNPYQVRPNDPEWAHLRDATWDRVGSKDFKAGYGPLTEILLRWTYR
                     AASAVTPDSHLQAFWFKLPFALFDLGVILALRALLRARGLPEARVLIYAWSPLPVMEF
                     WNSGHNDSMAVFFVVLALLAAAKERWTWAFGALTLAAAAKIWPLLLFPIFAGWCGRRP
                     LRWYQWWVCVPILGALCLPYWSDIGENLRFMTGFLSGWRNNDSLYGVLLWLTGDVYRA
                     KYTAFAVIAGVVVFVTFRRVPLEKASLVAIVATLMISANCHPWYLTWILPLLAFYPLP
                     ALLLWTAVMPLAYEVLIGWTILGEWNGSTPLRWWIYVPVYGLLIFGWLLRLRETHHGT
                     ETRARY"
     gene            10316..11932
                     /locus_tag="VLE22_07565"
     CDS             10316..11932
                     /locus_tag="VLE22_07565"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HSB14302.1"
                     /translation="MLSTLSALSFLVAPSPMRQVMNNLNDDTFAGIHQLSWFDWSLLI
                     PYFAILIVLAIYGSHRYEVIRTYYKHRKKLVQEPTRWFEKLPPVTIQLPIFNERYVIE
                     RLIEEIVKVDYPKQLLQIQVLDDSTDDTHPFTEALVEQYRALGHPIEYHHRSNREGFK
                     AGALQAGMQTATGELIAIFDADFVPPPDFLMRTVHFFAEPDVGVVQTRWSYLNRDYNV
                     LTEVQAMLLDGHFILEHTARCSRGLFFNFNGTAGILRKKMIEDAGGWQHDTLTEDCDL
                     SYRAQLKGWRFVYLPALDCPSELPVEMHAFQVQQSRWAKGLTQVSKKLLPSILRAPIP
                     VREKIEAFFHLTPNLSYPLMIIVSALMLPVMIVRFYIGWFQMLVIDLPLMIACFWSIV
                     AFYLLAQRELSPMSWKRSIFMLPALLAVGVALAVINTRAVLEALFGIQSSFVRTAKYA
                     LAGDRVRVADTTYRSRSGFLPYIEMAIGTYFLYMVYFAIESYNFLALPFLLIFVTGYY
                     WAAISTLWHEHQGRARWQRQRRLELEAAAR"
     gene            complement(11939..13372)
                     /locus_tag="VLE22_07570"
     CDS             complement(11939..13372)
                     /locus_tag="VLE22_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HSB14303.1"
                     /translation="MMTGFVSEKTRNWIWKAIVPVALYALVVWAIPRPEALKPESWRL
                     VGIFLATIAGLILQPIPGGALVLLAVTLASILGGLTIQDALAGYGDPTVWLVMAAFFI
                     SRALIKTGMARRLALFFVRLFGKTSLGVCYALSLSDMTLASIIPSNAARSGGVILPIL
                     RSIAEVYGSRPGATAALLGSFLMTAVYQSICVTAAMFFTGQASNPLAAQIATNTFHFP
                     VTWTSWLVAGIVPGLVSLALVPLVVFRLNPPEIKRTPEAAAFAARELDAMGPMSGGEK
                     LVAAVFVTVCGLWVTSGLHKIDITVTALLGSSALLFSGVLSWEDVKSEKAAWDIFIWY
                     GGLLRLGKALGDAGATTEFAKAVGSVLAGANPVLLFAAALLIYFYAHYGFASITAHIL
                     AMYPAFLAVLVAKGAPIGLVVFAFACFTNLSAGLTNYGTTPSPMFFAHEYVSLKKWWL
                     VGFVTSLVNLAVWSTVGFAWWKLIGIW"
     gene            13428..14816
                     /locus_tag="VLE22_07575"
     CDS             13428..14816
                     /locus_tag="VLE22_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSB14304.1"
                     /translation="MRTLCLALLFFAPHAPAAETSLTERARGYLIELIRRDTTNPPGN
                     ETRVAEYLKQVADAWGIPCELLGDDPARLNFVARLSGTGDGRPLLLMAHTDVVPADRS
                     QWTADPFAGEIRGGYIYGRGTQDDKGLLAAELAVLVELKRRDIRLKRDVILLAEADEE
                     AGSTGMRWLIRNAWSKIDAEFALNEAGSSMDLPSGVRLFQIQTSEKIPTRVVLSARGT
                     AGHGSLPRADNAVVRLARAILRLADADQPVHLNTTTRRYFKEIARLPDYRWLVPLLPR
                     LENPSSAIAAANQIRAKETELSAMLRTTVSPTMLSAGEKINVIPNVAEAQIDVRRLPN
                     ETAEEVLDRFRRVLNDSAVEVRPAGGQEMPSTEPSSLTTVLYKAMERVLRRSHPKAAV
                     VPFMIRGATDGAFLRQKGMAVYGVPIFAREGNESRSHGNDERVAEASLSTGSELLWQI
                     VVAVAAEDGAVR"
     gene            complement(14823..16046)
                     /locus_tag="VLE22_07580"
     CDS             complement(14823..16046)
                     /locus_tag="VLE22_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSB14305.1"
                     /translation="MRQGILNRFLIVTISLLLGTAGFAASQTKKKRATTRKPAATTAK
                     KSTAASVSKPVSSPTVLRSTTSRKPARARVRRTRRSYSPWSEPTFADSTIGDVIDGED
                     PVVRRAAVEALGPYNGAVVVVDPNTGRVLSMVNQRLALKSGFQPCSIIKVPVAMAALV
                     EGIIENNTVMRVYGRKRLTLTEALARSDNPFFAHLGNKLGFDRFSHYARLFGLGEKAG
                     LDIIGEQPGVFPAEPPRNVNLGIMTSFGEGISLTPLQLAAMLSSIANGGTLYYLQYPR
                     SAEEALSLVPRVKRHLDIESWIPEIKPGMMAAVEYGTARRANYDPTEPILGKTGTCTD
                     ARTHMGWFGSFNDVGRNKLTVVVMLTGGRGVNGPTAAEIAGNVYRNLSQQDFFAHNRE
                     ISPTALVGAGSCCSQ"
     gene            complement(16188..16937)
                     /locus_tag="VLE22_07585"
     CDS             complement(16188..16937)
                     /locus_tag="VLE22_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSB14306.1"
                     /translation="MSTPFRLDGRKALITGGASGIGEATSRAFAAAGASVLIVDIDRQ
                     RAETLAAELPDASPLICDITNESAVNEMFASISALDALVNNAGIGLVGNIEETCLADF
                     QRLLRVNVEGMYLVTKYALPLLLAAQGSIVNIGSVAGLVGVKRRYAYCATKGAVVATT
                     RQLAVDYPTQLRVNCICPGTVDTPFVEAYLERYHKHEKEKVRAELNQRQPVGRLGRPD
                     EIAYLALYLSSPEAGFVNGSVITIDGGWTAA"
     gene            complement(16978..18069)
                     /locus_tag="VLE22_07590"
     CDS             complement(16978..18069)
                     /locus_tag="VLE22_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HSB14307.1"
                     /translation="MRNVRSLLSFLPVCAAIVLFVAMSLRLYAGPPGDGWELVFHDEF
                     NGPRLNWDVWMCETGDRRNAYNDPDDSYLDGRGHLVLRVRKVDGKYHLGFIRTKQEYQ
                     WGYYEARTNLDVVPGYWSAFWLWGRQSYNPNYDGAEVDILEDPYRDEIVEHNIHQGQN
                     NTHKHEGARIAVKGSRTDWHLFACDWHEEGFDFLVDGQETWITRTMKASKPNWIYLTL
                     EAKNDGWAGDIRQSDLHLPAYWTVDYVRYYRRNPQAPAISLSQPEPIFQGKEDGKTIQ
                     AALRNGKFVERLNPRSWSVENLPAGVTLAVVERVDDTRVNLKLKGNAEAGPRAADITD
                     VTVIAEAGEVANSSALLIASRGIKLDGKR"
     gene            18195..19328
                     /locus_tag="VLE22_07595"
     CDS             18195..19328
                     /locus_tag="VLE22_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14308.1"
                     /translation="MSESIGIGMIGYKFMGKAHSNAYRQVPQFFDTPVKPRLKVICGR
                     DQAKVERMAQRWGWETAVTDWRRVIDDPDVKIVDICSPNNTHHDIAIAAFEAGKIVAC
                     EKPLAMNGAEAKTMAAAARKSGKINTVWFNYRRVPAIAFARQLIEEGRIGRVYHFRAL
                     YLQDWTLDPSVPLVWRMDKDVAGSGVTGDLLSHVVDISNYLAGEIEAVAGTSRIFVGE
                     RERPEGGSAKVEIEDAAIFVCEYANGAIGHFEATRFANGRKNGNIFEINGEHGSLYFN
                     LEDMSRLWYFDSREPKHVQGWRQISVWEEIHPYMKHWWVPGCAIGYEHTFGNQAADLL
                     SAVAAGKPMKPDFDDGLKCQVVLDAVLEACETDRWVKVKPVGA"
     gene            19357..20253
                     /locus_tag="VLE22_07600"
     CDS             19357..20253
                     /locus_tag="VLE22_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB14309.1"
                     /translation="MKISLGSWAFSFGPYADNPIPFDKTARRLSEAGYDGIEICGFPP
                     HITLDRYPTKKSRREVVRLLADLNLGVSGYAADLTSVNPVVAGNRGEYLEVLRRNVEM
                     CADIGSPAIRVDSVAAPGSIADSDYQATFARVASLWAEAAEIAQRGGIRMVWEFEPGF
                     VFNKPSEVVAMHDRVGHPNFKVMFDTSHAYMCGVVGARQHGAKETLPGGVPEFLKKLE
                     GRIGHIHLIDSDGTLHGDETSTHRPFGEGYIDFKGLAPQLLRVPNIEWWCIDLCFWAN
                     AWGLVEPSLKFVANLIDEKQAA"
     gene            20323..21939
                     /locus_tag="VLE22_07605"
     CDS             20323..21939
                     /locus_tag="VLE22_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSB14310.1"
                     /translation="MLSYTRGPEAPLLEKTIPQVLAETVARFPDRDAVIVRHQNARMT
                     WLELARSVEQTARGLSGLGLQPGDRVGVWSANCVEWILLQYASARAGVVLVNVNPAYR
                     THELRYVLGSSKIRALFLWERDARADYLQILRDSCNGGGLPLDHVILLGQEPWNRMLA
                     RGVDVPPHAASPEDVANIQYTSGTTGAPKGVLLTHRNLLNNGALIALCLGADEHDRIC
                     IPVPLYHCFGCVIGTMAAVTSGAAMIVPSAQFDARATLQAVHAERATAIYGVPTMFIA
                     ELDHPEFASFDLTSLRKGVMAGSPCPIEIMKRVVSDMHCEGMTIAYGQTESSPVITMS
                     RVDDSIELRVSTVGRAMPNTEVKIVSTGTGETVPLGEQGELCTRGYLVMKGYDGDPEA
                     TGEAIDSEGWLHTGDLAVMQPNGCFRMTGRSKDTIIRGGENIYPREIEEFLYTHPKVA
                     DVTVVGLPDARLGETVCAWVRLKNGSAATAEEFREFCRGKIAYFKIPQHIRFVDAFPM
                     TVSGKIQKFRIRETEIRDRGLEQAAAIETA"
     gene            complement(21973..22797)
                     /locus_tag="VLE22_07610"
     CDS             complement(21973..22797)
                     /locus_tag="VLE22_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HSB14311.1"
                     /translation="MQPSEQEQAHSDWENPVRKTLKEGKPVVGVTITVPSVELAANAA
                     NLGFDFLWIEMEHSPITLETARNMILATRGLKAVPFTRVPVNELWTAKRVLDSGSLGV
                     MFPFTSTPELARQAVAACKYPPEGRRGSGPGLAAFRWPAPEGYHQFANRNVMVIVIIE
                     QAQAVENVEEIAAIPGIEVLFIGTSDLSYSLGVGGQTDHPKVKEAVARVVAAGKRNNV
                     PLGRPAPTADLMQKYMEEGFLFFQAPADLVIFSNGARQILQPLGKTGLDPRTRLLY"
     gene            22970..23617
                     /locus_tag="VLE22_07615"
     CDS             22970..23617
                     /locus_tag="VLE22_07615"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSB14312.1"
                     /translation="MNATKEKILDAAEGLFAEHGFGATSLRQITAQAGVNLAAINYHF
                     RSKDALIQAVFSRRIGPMNEKRLAMLDACEAENANGPLPLVPVLRAFVVPIFELCREL
                     GDRGRSFRRLMGRMYVEPGDTVRKILFEQMQIIAGRFIAAFQRSLPHLPMVELLWRIH
                     FCIGVISHTLAGTNHLEVVSKGLCDPFDTEATTERVVAFLVAGMRAPLPAMPTRS"
     gene            23623..24966
                     /locus_tag="VLE22_07620"
     CDS             23623..24966
                     /locus_tag="VLE22_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB14313.1"
                     /translation="MRTFHLNWLPLLLLPALVPAAAQTAGTPLELSLKHAIEIALAPD
                     GNARVQIARQMIRQAQERANQARATLLPNLDASVSQQNQTRNLAAFGIRIEAPIPGFR
                     IPELVGPFNTFDARATATQTIFDFGAIRRYQASRAGIGAAGAENDATRDQVALQVAGL
                     YFASLRAEARVQAAEANVKLAEELLQLAENQKTAGTGTGLEITRAQVQLANERQLLLV
                     ARNERRQANLQLLKAMGLPLVTPLLFSYQLARVPVEETTPEKALAVALESRADLKAQR
                     QREESARLSYSGAKMERLPSVVGFADYGTIGTSIHNTVPTRTYGLSVRVPVFDGGRRD
                     ARRAESGSLFEQEKIRTADLKRQIDLELRLSLDSLRSAEEQVNVAEDGLKQAEDELAR
                     ASRRYKAGVANSLEVTDAQTRLARARDNRISALYLYNQARVELGQALGTIQQMVD"
     gene            24982..26169
                     /locus_tag="VLE22_07625"
     CDS             24982..26169
                     /locus_tag="VLE22_07625"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB14314.1"
                     /translation="MKKKIIPIVILAAVLVIGGGYLYRWLNRNDNGRILISGNIELTE
                     VKVAFKTAGRLVERAYDEGDTVKKGAVAARLDREQLERQLERERATLAGAESALVQLR
                     TAIAYQRETLDGETAARTAELGQADARLRELSTGSRPQEIQDAKAAVEAARTEQARAR
                     NDWERAKVLFQNDDISASQNDQFRTAYERAAALLQQAEQRLALVTEGPRQENIDAAKA
                     GVERARATVRLAQASRLELKRKEQELGARRADIDRAKAQIAVIESQLNDSAVTSPIDG
                     VVLVKSAEVGETLGAGATVLTIGDLDHPWLRGYINETDLGRVKLRSKVKVTTDSFPGK
                     VYWGTVSFISSEAEFTPKQIQTPEERVKLVYRVKVDLPNPGHELKSNMPADAEILVAD
                     AGR"
     gene            26181..27119
                     /locus_tag="VLE22_07630"
     CDS             26181..27119
                     /locus_tag="VLE22_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB14315.1"
                     /translation="MDSAIIEATDLTRRFGTLTAVDRLNLRIAPGEIFGLVGPDGAGK
                     TTTLRLLCGLLDPAAGSSRIAGHDSARETDQVKDAIGYMAQRFGLYGDLTVEENMKFY
                     ADLFGISRPERDEMTPNLLRMTRMEPFRTRQAGRLSGGMKQKLALMCTLLHKPRILFL
                     DEPTNGVDPVSRRDFWEILYQLVREGLTVFVTTAYLDEAERCDRVGLMHKGRLIRCDA
                     PDKLKSTIPEPCYEVGCPDLRAAKEFLTRQAGVESVEPAGASLHLFLSPERTSADALA
                     GALQAAGLGPANFRRIAPSLEDAFILLIHQASREAA"
     gene            27116..27865
                     /locus_tag="VLE22_07635"
     CDS             27116..27865
                     /locus_tag="VLE22_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB14316.1"
                     /translation="MSNNGWAVEVDRLVKRFGKFVAVDHITFQVRRGEIFGFLGPNGA
                     GKSTAIRILCGLLQPSEGLARVGGIDVSKDPETIRRNIGYMSQKFSLYDDLTVEENIE
                     FFGGVYGVPEEQLAARRDHVLEMAGLTEQHAMMTRLLPGGWKQRLALGCAILHEPPIL
                     FLDEPTSGVDPIARREFWELIYRLSAAGHTVFVTTHYMEEAEYCHRLALMYRGKIIAL
                     GAPAELKRSAAKPTMEEVFVSMIEEEERRGA"
     gene            27862..29007
                     /locus_tag="VLE22_07640"
     CDS             27862..29007
                     /locus_tag="VLE22_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14317.1"
                     /translation="MKFRRSLARTVSIARKEGLHIVRDPRSLIMALAVPFVMLLLFGY
                     ALTLDVDRVPTLVYDHDRSPQSRELIERFAGSRYFRILGSVDEYGGIERSIDRDQALL
                     AVVIPADYSRNLLTSKPAEVQLLLDGSDSNTASIALGYAESLVQMYAFELRARAIDRR
                     GGQQLKPPVETRLRVWYNSELQSRNYIVPGLIAVILMIIAALLTSLTIAREWEMGSME
                     QLLSTPLRAHELVLGKMLAYFALGLIDMVASIVIGIQLFGVPLRGNPLLVLVSGGVFL
                     VGALFWGILVSALARSQLLAYQLGMVTSYLPAFLLSGFIFAIESMPAVIQGITYIFPS
                     RYFIMILKGIFLKGVGIQVLWVDFAFLLAYASIVFVVATKKVGQKLA"
     gene            29012..30169
                     /locus_tag="VLE22_07645"
     CDS             29012..30169
                     /locus_tag="VLE22_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14318.1"
                     /translation="MWRRIREILKKEFRQVLREPRMRVVLFVPPLLQLIIFGYAVNLD
                     VEHSRIAWMDQDRTPESRELLAAFAASDRFRVVATPGSEEEVQELLDGSGVQMVVRVL
                     PGFGRDVQRGETASVQVLVDGTNSNTASIVAGYAGQVIASFGNRVMHEQQDARLVGQT
                     AVAGGPVNTDLPVLQARTRVWFNPDLLSRNYFVPGVVVNIISLVTLMLTALAIVRERE
                     IGTMEQLMVTPIRPIELILGKTLPFAIVGLLDLVLITTAALLVFQIPFRGNPLILLLA
                     SVLFLLTTLGVGLFISTISQTQQQAMMASFFFFMPTFMLNGFAFPIRNMPVPVQYLTY
                     LNPVRYFLEITRGVFLKGTGLEALWPQMLALLVFGVTILTVSALRFHKRLD"
     gene            30249..30632
                     /locus_tag="VLE22_07650"
     CDS             30249..30632
                     /locus_tag="VLE22_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HSB14319.1"
                     /translation="MRFLILLLMALAPLWPADLNPEQQQRAHKLETSLLAPCCWSEPV
                     ATHRSEISLSIKAEIEKLVGEGKTDREILDVYKSRYGMRIFVEPEGSQWWVMNVVPVV
                     MTILGVLVVIFVIRRMLRPIPASSQ"
     gene            complement(30604..30984)
                     /locus_tag="VLE22_07655"
     CDS             complement(30604..30984)
                     /locus_tag="VLE22_07655"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HSB14320.1"
                     /translation="MIFEVSSRKTLDQIGQGLQESAARHKFGVIAVHDLKETMKKKGV
                     DFAGECMIYEVCNPHQAKKVLEANGAVSTALPCRISVYRSGDIYKLATILPTAMMKIF
                     GNPELEPVAREVEEVVTAMMRESA"
     gene            complement(31006..31860)
                     /locus_tag="VLE22_07660"
     CDS             complement(31006..31860)
                     /locus_tag="VLE22_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSB14321.1"
                     /translation="MPPQGFQNILCPVDFSDTSALGLRFANALANCAGARLYVLYADQ
                     FLPPPYFTHDRLEQIRKQLQDAKLEAENSLKEFTGKTLGVAGPGVSVRVIEELPVDGI
                     LQAIDQTSADLVAMGTHGRSGFNRLMLGSVTERVLREAKIPVLTVRPYGNGPEPFPSI
                     RSVVCPVNDSDVARAALGYAAKLAHCFGAELTVLHVRESHAKDAIADLCEWVPAEVRS
                     LCSVREMMRDGEASEIIVSAASELPCDLLVLGARHRMFFDSTVIGTTSVRVVRHAPCA
                     VLTVPAQD"
     gene            31976..32368
                     /locus_tag="VLE22_07665"
     CDS             31976..32368
                     /locus_tag="VLE22_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14322.1"
                     /translation="MADHVKILAVLHIVFGVLGLLAALLILVIFGGVAGIVSVSEASH
                     DPNAYLAVPILGVVGTIIFGLLLLLSLPGVLAGVGLLKFQPWARVLTIVLCAINLLNV
                     PFGTALGIYGLWVLLNSETEQLFAARRV"
     gene            32476..33357
                     /locus_tag="VLE22_07670"
     CDS             32476..33357
                     /locus_tag="VLE22_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSB14323.1"
                     /translation="MANELAALSNELATVVERAGQAVVAVHARPRFSSSGAFWRPGII
                     VTAEHTIRLEEEITVTLPDGSNVPATLAGSDAGTDVAVLRVDGAAGSPISRAGAVPSP
                     GNLALAIGRSQDSGVNATMGIVSAVSGSWRTWRGGRLDHYIRLDLTLYPGSSGGVAVN
                     IEGEAIGIATSALSRIAGVAIPASTVDRVVDEILKRGRVARGYLGVGLQPVELPDHQK
                     GLIVLSVESGGPADKAGVLIGDILISLGRHVVVDTDDIQTVLESQGVGRSVETELLRG
                     GVAKKLAITTGERSRRS"
     gene            33359..34153
                     /locus_tag="VLE22_07675"
     CDS             33359..34153
                     /locus_tag="VLE22_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSB14324.1"
                     /translation="MRGFGEISERLRRSTARVFAWERGGGGSGVVWSADGLILTNAHV
                     ARTPAARVELWDGRCFDARVVARDPRRDLTVLRISADGLEAAMPGDSASLRPGELVMA
                     IGNPLGFIGALSTGVVHSIGPLPGMGHERWILADVRLAPGNSGGPLANAQGQVVGINT
                     AIVNGLGVAAPVSTAMDFLRRGARPALGATLRPLRFGLLILEVDPGGAAVAASLRAGD
                     ILLTSLGELSDALDSGLDVMRLRFLRGNRERVREVCVQLRAHAEAA"
     gene            34150..>34679
                     /locus_tag="VLE22_07680"
     CDS             34150..>34679
                     /locus_tag="VLE22_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB14325.1"
                     /translation="MIRLLIVASSAVVRAGLEALAASNPGLQVVGSLPDFTGVEDVHP
                     NVVLAAAPLEEIPPLPGGSAVPLVLITSDSQAVWTLEALRLGVRALLPRDASQAEILA
                     AVEAAATGLAVIDPQDLDAMLSSAAPVQAAGDVSALTARELEVLRMMAEGAANKIIAW
                     KLGISEHTVKFHVASIL"
BASE COUNT         6628 a        10713 c        11078 g         6260 t
ORIGIN      
        1 ccggccagtt cgagcgaacg ctcatcgtgg ccgaagaggg cgcctacgtc agctacctcg
       61 aaggctgcac cgcgcccatc cgcgacacca accagcttca cgccgccgtg gtcgagctcg
      121 tcgcgctcga taacgcccag atcaagtact cgacggtgca gaactggtat ccgggcgaca
      181 aggacggcaa gggcggaatc tacaacttcg tcaccaagcg cggcaagtgc cagggcaaga
      241 actcgaagat ctcctggacg cagctcgaga ccggctcggc gatcacgtgg aagtacccga
      301 gctgcctgct cattggcgac ggttcggtgg gcgagttcta ttccgtggcc gtgaccaaca
      361 actaccagca ggcggacacg ggcacgaaga tgatccacat cggcaaggac acgaagagta
      421 cgatcgtctc caagggcatc tcagccggac acggccagaa cagctaccgc ggcatggtga
      481 agatgctgaa gggcgcggag ggcgcccgca actactcgca gtgcgactcc ctgctgatcg
      541 gcgaccagtg cggcgcgcac accttcccgt acctggaagt caagaacccc acggcgcagg
      601 tggagcacga ggccacaacc tcgaagatcg gtgaggacca gatcttctac tgccggcagc
      661 gcggcatccc gaccgaggac gcggtctcga tgatcgtcaa tgggttctgc aaggaagtgt
      721 tccgcgagct gccgatggag ttcgccgtgg aagcacagaa gctgctgagc gtgagtctgg
      781 aaggtagcgt cggataagca gttcgatgac aaaggcacag aggacaaaga cggaaatgct
      841 gattatcagg aatttgcgag ccggcatcgg cgataaggaa atactcaagg gcatcgatct
      901 ggaagtgaag ccaggcgagg tgcacgccat catgggcccg aacggctcgg gcaagagcac
      961 gctggcgcag gtgctggccg ggcgggattt ctatgaagtc accggcggcg aggtgatcta
     1021 cgaggggcac gatctgcttg aactcgatcc ggaggagcgg gccgcggagg ggatcttcct
     1081 ggcgttccaa taccccgtgg agatccccgg cgtgagcaac atgtactttc tgcgcgcggc
     1141 cctcaacaat atccgcaagc ggagcggcgt ggaggaactg gacgcggtcg acttcctgga
     1201 gctggtaaag gaaaggatga cggctttgga catgccggag ggccttctga accgtgcagt
     1261 gaacgtcgga ttctccggcg gcgagaagaa gcggaacgag atcttccagc tcgccgtgct
     1321 ggagccgaag ctggcgattc tcgacgagac cgactccggt ctggacatcg acgcgctgag
     1381 gctggtggcg aacggcgtga actcgcttcg cagcccgaag cggtccttcg tcgtggtgac
     1441 gcactatcag cggctgctga actacatcgt gcccgacttc gtgcacgtca tgtcgaacgg
     1501 gcgcatcgtg aaatcgggcg acaaaacgct ggcgctcgag ctcgagcaga agggctacgc
     1561 ggaactggac gagctggcgg tgagcgagct ggtgcgatga tcgaactcag agaagatcag
     1621 gacgtttatc tcacgcattt cgcggaccgc gggcagcgga cggcggcgga accggaatgg
     1681 cttcgcgatc tgcgcaggag cgcgatggac cggtttgccg cgctcggctt cccgaccacg
     1741 cacaacgagg actggaaata cacgaacgtg tccgggatcg cgaggctggc ggcgcgtccg
     1801 gcgcccgcgg ccaccctgac gcgcaaagaa ctggaacggc tgccgttcgc gaacctgggg
     1861 tgcggcgtgc ggctggtgtt tctgaacggc cgattctcgc cgaaactgtc cgctgcgaag
     1921 cggctgccgg cgggcgtcaa cgcagcaagc ctagcgtcgg tgttggagaa cggaagcgca
     1981 cctttggaaa accacctcgc gcgttatgcg ggcttcgagg aaaacgcctt cgtcgcgctc
     2041 aacaccgcgc tgtttgagga cggcgcgctc atcgagattg caaaaggtac cgtggttgag
     2101 gaaccgatcc acctcgtctt cgtctcggtg gggggcgccg aaccgtgggt ggcgcacccg
     2161 cggaacctga ttctggccgg gcgcgggagc caggcggcgg tgatcgaaac gtacgtcgcg
     2221 tcgggtgagg gtgtccactt caccaacgcg gtgacggaga ttgtagccgc tgagggtgcg
     2281 gtaacggaac actacaaact tcagcacgaa ggggcacagg cgttccacgt tggcacgatt
     2341 caggggcttc aggagcgcag cagctcgctt acgtcacaca acgtcggctt cggcgcggct
     2401 ctggcacgaa acgacgtcaa cgtcgtgctg gacggagaag gcgccgagtg tatgctcaac
     2461 ggcctttacg ccaccacggg caaacaacac gtcgacaacc attcgacgct ggaccacgcc
     2521 aagccgaact gcaacagccg cgagttatac aagggcatcc tggacgggca gggcacgggc
     2581 gtgttcaacg gcaagatcat cgtccacaag gatgcgcaga agaccaacgc gctccagagc
     2641 aacaagaacc tgctgctttc agcggacgcg atgatcaaca ccaagcccca gctcgagatc
     2701 ttcgcggacg acgtccgctg cacacacgga gcgacggtgg gccaactgga caaggacgcc
     2761 ctgttctaca tgcagacccg cggcatcccg agggaggctg cgcgggacct gctgacctac
     2821 gcattcgccg gcgaactgtt cgaccggatg aactgggctc cggcgcgcga gcggctggag
     2881 caggagctgt accggaagct gtcgcaaggc aggaagccgt gagcttccag gcgaccggag
     2941 cacgcatgaa aaccgcaagc gagacagcgt tcccggcgag ccctgaggcc gctgggggat
     3001 tcaacgtgtg gcgcatccgg gaggacttcc cgattctgcg ccggcaggct tgggacaagc
     3061 cgctggtcta cctcgacaac gccgccacca ctcagaagcc gcaagcggtg attgacgcga
     3121 tcacctcgtt ctacacgtcg gattgcgcga acatccaccg cggcgtgcac ttcctgagcg
     3181 aacgagcgac ggacgcctac gaggagagcc gcgcaaaggt gcggcgcttt ctcaacgcgc
     3241 gcgacacgcg agagatcatt ttcgtccggg gcgccacgga aggaatcaac ctggtggcgc
     3301 ggagcttcgt ccggccgatg ctgaaggaag gcgacgaagt tctgatcacg gcgatggagc
     3361 accacgcgaa catcgttccc tggcaaatgg tctgcggcga gacgggcgcc cggctgcggg
     3421 tggccccgat cgacgaccgc ggcgagctga tcatcgacga gttcgagaag ctgttgggtc
     3481 cgcgcacacg gttcgtcgcc gtgagccacg tctcgaacgc actgggcacg atcaacccgg
     3541 ctggcatgct ggtcgaaatg gcgcgctcgc gaaaaatacc ggtgcttgta gacggggcac
     3601 aagccgtacc gcacatgaac gtggacgtgc aggcgctcgg ctgcgacttt tacgtgtttt
     3661 cggctcacaa gctgtacggg ccgacgggca tcggggctct ctacggacga gcggagttgc
     3721 tcgaggcgat gccaccttac cagggcggcg gcgacatgat cgcctcggtg acgttcgaga
     3781 agacggtgta caaccaactc ccctggaagt tcgaggcggg cacgccgaac atcgcgggca
     3841 cgatcgggtt gggggcggcg atcgattacg tgagcgcgat cgggttggac gccatcgcgg
     3901 cgcacgagca tggattgctc gcgcacgcca ccggaacgct ctcttcaatt cccgggttga
     3961 agatcatcgg gacggcgcat gacaaggctg cggtgatttc gttcatcctg gacggggttc
     4021 accctcacga cgtaggcacc attctcgacc gcgagggagt ggccgtccgc gtggggcacc
     4081 actgcgcgca accggtgatg cagcggttcg gcgtaccggc gacaagccgg gcgtcgtttg
     4141 cgatgtacaa cacgagcgaa gaggtggagg ccctgacgca agcggtatac aaagtgaagg
     4201 agatcttcgg ctgatgtcgg acctgcggga actctaccag cagctcatcg tggaccacag
     4261 caagagtccg cggaactgcc gcgcgatcga gggcgcggac ctgagcgcgg agggctacaa
     4321 cccgctgtgc ggcgaccatg tgacggtcta cgtgaagctg gagaacgacc gggtgaagga
     4381 catcgcgttt caaggcaacg gctgcgcaat ctccaccgcc tcggcctcgc tgctgacgga
     4441 ggtcctgaag ggcaagacgc gcgccgaagc cgaggcgttg ttcgaaagtt tccacgatct
     4501 ggtcaccggc cacgagtcac gggagaaaca gggcgcaccg caactcggga agctgcgggt
     4561 attctccggc gtgtcggaat ttccggtccg cgtgaagtgc gcgacgctag tgtggcacac
     4621 gctccgggcg gcgctgaacc agatcgagga aaaagtcagc acggagtaac tgtaactatg
     4681 ccggagagta acgaggcaga cacggacatc ctgagacaaa aggtgatcca ggtcctgcgg
     4741 cagatccacg atcccgagat tccggtcaac atttacgaac tcgggctgat ctacgacctt
     4801 tcggttgccg agacgggcga ggtgaaaatc cagatgaccc tgacggcgcc gagttgtccg
     4861 gtcgccggga gcctgcctcc ggaggtgaag tccagagtcg aggcgctgcc cggagtgaaa
     4921 gaggtcgagg tggagctgac ctgggacccg ccgtggagca tggacaggat gtcggacgcg
     4981 gccaggcttc agctcggcat cgaaccggtc gacgacggcg cgcgggtagt gaggttcgag
     5041 ccatgaagct ttccgcgcac gaagagtacg ggctgcggtg cctgcttcag atcgggcgtc
     5101 acggcgaccg cggcggcctg accattccgc agatcagccg gctggagggt ttgtcgattc
     5161 atcacgccgc gaagatcctc cacatcctga agcagaccgg gttcctgaag agtgtccgcg
     5221 gccaaaccgg cgggtacacg ctggcgaagc cgcccgagca agtcttcatc agcgacgtgc
     5281 tttcgacact cggcggaaag ctctacgaag aggacttctg cactgaacac agaggccgcg
     5341 tgggcaactg cacacacacg gtggactgtt cgatccgttc gctgtggcgc atgttgcaga
     5401 ccgcggtgga cgaggtgctg agtaagacga ctcttcgcga cctcatccgg aaagaggacg
     5461 agatgagcac ctggctggac cagttggtga cgcttccaac cgggaacgag ccggtgacgc
     5521 cgagggcgtg aggggaagca gtactgttgg ttccctccaa actgccaagt gcgcggaggg
     5581 tgctgacaag actaccagca tggccggtct ggcgaccggc tccacgcgaa catcggccaa
     5641 aagggcggac gtgggcgttc gccgcggccc tgggaaaccg ccctacctac ctggcacggg
     5701 agcgggttac gatgccttcc gtgtacgtct tgaacgcctc aggctgcccc ggtggggcga
     5761 tctcaaatct cacgcggacc tgatcggctt ccttgcggta gatcaagcga aacctcggtc
     5821 tccctggttc cttgtcgctg aggaacgtga tggtgttctg atccgtccca acatcaaccc
     5881 ggtactgaat ctgatgccct tcgttgtcga aataggaggc gcggagtccg gccggagacg
     5941 aaaagactac cgtaagatcg gtgtgtacaa atgccgggcg atccttggtg gccggatact
     6001 cggttcggcc ccggcgtatg agaatcttcc cgtcgagatc cttctcaaac gtgaatccac
     6061 cgctccccgc tcccggctgt ccctgtccgg acccggccca ctcgccgaga agaaagtgta
     6121 gaggctccaa tttgtcagtc gagttcggct gcgacatcat gcagcaactg cctagaaata
     6181 gcacccacac gcaccggatc atcattggga agcatagctc aaactcgcaa cgccggctta
     6241 gcgtacaagt tgcagctccc gctggcaggc agacgacaaa atgagatcgt ctgctccacg
     6301 tgcgctgccc caccttcagc accctagggt tttcgcacgc cgcggaagcc gcagaccttc
     6361 ccccgccagt agggatcgaa cgtcgacagg ttggttccct ttatcgactg ggcgtggtaa
     6421 ccgagtacga cgcagggcgg gagccctgtg ctcgcaggtc aggagacctg cgctactaca
     6481 cttagccgcc ggcgatagcc ccgatgatga ggaacggctc ggcgcccgat gcgaccgcgt
     6541 cgggcaacgg ggcgtccggc ggctcatgag acagatcctc gccgcaggcg aagaaccgca
     6601 ggaacgggcg gcgctgtttc gtgacttggt cgcggatcgt gccgcgcagc accggaaacg
     6661 cggcctcaag ggcgtcaagg accgaaagct gggtgagcgg acctttgacg gcgagttgca
     6721 cctcgccgtc cacaccagcc atatttcgca aatgtgccgg aagcgcgact cgaatcacga
     6781 cagtgtctgg acctcgacag aaagcacggc cggaagatcc cggacaatcg gcgtccaact
     6841 gtcgccggca tcggctgacg cgtacacctg cccgccggtg gtgccgaaat acacgccgca
     6901 cgaatcgagc gagtcgaccg ccatcgcgtc gcgaagcacg ttgacgtaac agttgctctg
     6961 cggcagaccc ttttcgagcg gctcccattc gtttccgccc gtccggctgc ggtagacgcg
     7021 tagtttcccc tcgggcggat agtgctctga gtcgctcttg atcgggacga cgtagatggt
     7081 gtccggctcg tgcgcgtgga cgtcgatgac gaacccgaag tcggtcggca ggttcccgct
     7141 gacctcgcgc cacgagtcgc cggcgtcgtc ggtgcgcatt acgtcccaat gcttctgcat
     7201 gaacagcacg cccgggcgcg acctgtgcat cgcgatgcgg tggacgcagt ggccgacctc
     7261 ggcggtcggg tcggggatgt gctcggactt cagtccacgg ttgatcggct tccacgttcc
     7321 gccgccgtcg tcgctacgga aggcgcccgc ggcggaaatg gcgatgaaga tccggccgga
     7381 gtcgctcgga tccagaatga tcgtgtgcag acccaacccg cccgcgcccg gctgccagtt
     7441 cggccccgag ccgtgcccgc gcagaccggc gagctcctgc cacgtctgcc cgccgtcggc
     7501 cgaccggaac agggcagcgt cttcgactcc ggcgtagacc gtgtccgggt cagtgagcga
     7561 cggttccagg tgccagactc gcttgaactc ccaggggtgg ggcgtgccgt cgtaccactg
     7621 gtgagtgccg ggaacgccgt cgtacacgaa cttgttgccc accggctccc aagtcttgcc
     7681 accgtcgttc gagcgctgaa ccacctgccc gaaccagccg cttgtctgcg aggcatatag
     7741 ccggttcgga tcggcgggcg accccttgat gtggtaaatc tcccaccctc caaaatgggg
     7801 gccgctgaca tcccacttcc tccgtttgcc atccgacgtc agaatgaacg cgcccttgcg
     7861 tgttccaacc agcacgcgta ccttgctcat ctctaactcc cctctgcgga atttgcgccg
     7921 atattcaccg gcaaccagca caatcaggcg gaaacactca tgaaacccag tcccaggaaa
     7981 tacgttgctc cttgtcagca aagatatcac accggggatg cgcgggacac gcccgcaaac
     8041 ctgccaaaca tggccggtct ggccgcagtt cccccggggg acacccggaa aatcagtagg
     8101 gcgctacgcg agattcgaat tgcatggaat tgaagtcctg gagcacctcg gcggccgcgg
     8161 gtcctaaggg gttcacaagg gcgcgataca ggatgccttt ctccgcccac accagtgcag
     8221 ggccgtcgcg atccgcccac tcccaagtgg gcactgaaag tagcgctttg cgccccgggt
     8281 gttccagctc gtgctcgtcc cagtagacac cggccccttc ggggtgatcg atatcggcgt
     8341 aggcgaactt gcgcaggatc caaccgtgcc gaagcggcct ggtgaacacc gtgcaaggag
     8401 cgaccacccc agggcccgcc ttcatctcca gcgtccagcc gtcgcgcaga aggcggcggt
     8461 aatagacgct ggggcattcg gccccgaaga atccggcggg cttgtattcc tggtcttcgg
     8521 cgagttcggc gctcttccgc aggacgaagt ggcagccgtt cagccagtac gtcgtgttgg
     8581 atgtgaacag cccgccgcct tgccaacagt cgcccttacc gtaaagcgtg acggcgcgca
     8641 gccagggcgc gcgtgagatc gcggtccagg aacccttcgt ctcctgatgg cgacggcccc
     8701 cgcgcgcgaa gtagatgagg tggcggccgt cgggggagag gtcggcgcgt cgctcgtaga
     8761 tccggcccct cagccactgg ccgagcttga actcgccggt ttcgcgattc cagccgacgg
     8821 tgcagacggc gttggccgga cctcgccgga agacgacgcc cacaggcgct ttcgaagcca
     8881 gcagaacatg caggcgggct gggaacttgg gcgtcatgat cttttctctc agtatcgggc
     8941 acgcgtctca gttccgtgat gggtttcccg caatcggagc agccagccga agatgagcag
     9001 cccgtagaca ggcacgtaga tccaccaacg taacggcgtc gagccgttcc attcaccgag
     9061 aatcgtccag ccgatcagca cctcgtaggc caggggcatt acggccgtcc atagaagcag
     9121 ggccggcagg gggtagaaag ccagcagcgg cagaatccac gtcaggtacc acgggtggca
     9181 gttcgccgag atcatcagcg tcgctacgat agccacgagt gacgcttttt cgagcggcac
     9241 ccggcggaaa gtcacgaaca cgacgacgcc ggcgattacc gcgaacgccg tgtacttggc
     9301 ccggtaaacg tcgccggtca gccagagcag cacgccgtag aggctgtcgt tgttccgcca
     9361 accgctgaga aagccggtca tgaagcggag gttctcgccg atgtcgctcc agtagggcag
     9421 gcaaagagcg cctaggatgg ggacgcacac ccaccactga taccagcgca gcggccggcg
     9481 cccgcaccat ccggcgaaga tcggaaacag gagcagcggc cagatcttcg cggcagcggc
     9541 cagcgtgagg gcgccgaacg cccacgtcca gcgctccttg gccgccgcga gcaaggcaag
     9601 cacgacgaag aacaccgcca ttgagtcgtt atgtccggag ttccagaact ccatgaccgg
     9661 caggggcgac caggcgtaga tcagaacgcg cgcctccgga agcccgcggg cgcgcagcag
     9721 ggcacggaga gcgaggatga cgccgaggtc aaacagcgca aatgggagct tgaaccagaa
     9781 cgcctggagg tgtgaatccg gcgtgacggc cgaggccgcg cggtaggtcc agcgcagcag
     9841 gatctcggtg agcgggccgt agccggcctt gaagtcctta ctgccgacgc ggtcccacgt
     9901 tgcgtctctg aggtgcgccc actccgggtc gttcggtcga acctggtagg ggttgccgcc
     9961 gtacacctgc aacttgcctt cccagcggta gcgaaacaga tcgtcggaga gcgccggggg
    10021 aagcggccac acggtcagcc ggaacaacgc tgcaccggct gcaataagaa tccacaacct
    10081 attgtatttt atagacttat tggctttctc aattcgaagg atcaaatacg cgcaaacgat
    10141 gtagactatg gaggacaata gaaacaggac gattgcctga accaggtggc cgcgcaggtc
    10201 ccctagccgc cgggtaagaa tcaggataat ctccaggagc gctgccagcg tgacaaggct
    10261 agtgagtgtg acgcttattc tggactgttc tgttttcatc taaaatcgag aattcatgct
    10321 atcaactctt agcgcgctgt cgtttctggt tgcgccatcc cctatgcgcc aggtgatgaa
    10381 caacctgaac gacgacacgt tcgccggcat tcaccaactg agctggttcg actggtccct
    10441 gctcatcccc tacttcgcga tcctgatcgt gctggcgatt tacggttcgc accggtacga
    10501 agtcatccgg acttattaca agcatcgaaa gaagctggtc caggaaccca ccaggtggtt
    10561 cgagaaactt ccgccggtca ccatccagct tccgatcttc aacgagcgat acgtgatcga
    10621 gcggctgatc gaggagatcg tcaaggtcga ctacccgaag caactgctcc agattcaggt
    10681 gctggacgac tcgacggacg acacgcatcc gttcaccgag gcgctcgtcg aacaatacag
    10741 ggcgctcggg catccgatcg agtaccatca ccgctcgaac cgcgaaggct tcaaggcggg
    10801 cgcactccag gcggggatgc agaccgccac cggcgaactg atcgcgatct tcgacgccga
    10861 cttcgtgccg ccgccggact tcctgatgcg gacggtgcat ttcttcgccg agccggacgt
    10921 gggcgtcgtg cagacgcggt ggagttacct gaaccgcgac tacaacgtgc tgacggaagt
    10981 ccaggccatg ctgctagacg gccactttat cctcgagcac acggcacggt gctcgcgcgg
    11041 gctgttcttc aatttcaacg gcaccgccgg catactgcgg aagaagatga tcgaggacgc
    11101 cggcggctgg cagcacgaca cgctcaccga ggattgcgac ctgagctacc gcgcgcaatt
    11161 gaaaggctgg cggttcgtat accttccggc tctcgactgc ccgtcggagc tgccggtgga
    11221 aatgcacgca ttccaggtgc agcagtcacg ctgggcgaag gggctgacgc aggtatcaaa
    11281 gaagctgttg ccgtcgatcc tgcgggcgcc gattccggtc cgcgagaaga tcgaagcgtt
    11341 cttccacctg acgccgaatc tgtcgtaccc gctgatgatt atcgtctccg cgctgatgct
    11401 gccggtgatg atcgtccgct tttacatcgg ctggttccag atgctggtga tcgacctgcc
    11461 gctgatgatc gcctgcttct ggtcgattgt ggcgttctat ctgctggccc agcgggagct
    11521 gagtccgatg tcgtggaaac ggtcgatctt catgctgccg gcactgctcg ccgtcggcgt
    11581 ggcgctggcg gtgatcaaca cccgggcggt gctggaagcg ctgttcggca tccaaagcag
    11641 ctttgtccgt acagccaagt acgccctcgc cggcgatcgc gtgcgtgtgg cggacacgac
    11701 ttaccgcagc aggagcggat ttctgccgta tatcgagatg gcgatcggca cgtactttct
    11761 gtacatggtg tacttcgcca tcgagagcta caacttcctc gcgctaccgt tcctgctgat
    11821 ctttgtcacc ggatattatt gggcggcgat ctccacgctg tggcacgagc accagggacg
    11881 cgcgcgctgg caacggcagc ggcggttgga actcgaggca gcggcgaggt agcggcgcct
    11941 accagatccc gatcagtttc caccaggcga agccaacggt ggaccagacc gccaggttca
    12001 cgagcgaagt cacaaacccg accaaccacc actttttcag cgacacgtat tcgtgcgcga
    12061 agaacatcgg ggacggcgtg gttccgtagt tggtgaggcc ggcggagagg ttggtgaagc
    12121 aggcgaaggc aaagacgacc aggccgatcg gcgctccctt tgcgaccagc actgcgagaa
    12181 aggccgggta catggccagg atgtgggcgg tgatgctggc gaagccgtaa tgggcataga
    12241 agtagatgag cagggccgcg gcgaacagga gaaccgggtt ggcgcccgcg aggacagagc
    12301 cgacggcttt ggcgaactcc gttgtcgcgc cagcgtcacc gagcgctttg ccgagcctca
    12361 gtaagccgcc gtaccagatg aaaatgtccc aggcggcctt ctcgctcttg acgtcctccc
    12421 aactcagcac gcccgagaac agaagggccg agctccccag cagcgccgtc acggtgatgt
    12481 cgatcttgtg caggccggag gtgacccaga gtccgcacac ggtcacgaag acggcggcga
    12541 cgagtttttc gcctccgctc atcgggccca tagcatcgag ctccctggcc gcgaaggcgg
    12601 cggcttccgg cgtccgcttg atctccggcg ggttcagacg gaagacgacc agcgggacca
    12661 gcgccagcga gacgagcccc ggcacgatcc cggccacgag ccaactcgtc caggtcaccg
    12721 ggaaatggaa cgtgttggtg gcgatctggg ccgccagcgg attgctcgcc tggccggtga
    12781 agaacatcgc cgcagtgacg caaatgctct ggtagacggc ggtcatcaga aaggaaccga
    12841 ggagcgcggc agtggcccca gggcgagagc cgtaaacttc ggcgatggag cgcaggatcg
    12901 gcagaatgac gccgccggag cgcgcggcgt tagaggggat gatgctcgcc agcgtcatgt
    12961 ccgaaagcga aagggcgtag cagacgccga gcgaggtttt gccgaagagt ctcacgaaga
    13021 agagcgcgag ccgccgcgcc attccggtct tgatgagcgc ccgcgagatg aagaatgccg
    13081 ccatcacgag ccagacggtg gggtcgccgt acccggcgag cgcgtcctgg atggtgagac
    13141 cgccgagaat cgaggcgagc gtaacggcga gcaggacgag cgctccgcca ggaatcggct
    13201 ggaggatcaa gcccgcgatg gtcgccagaa agatgccgac gagccgccag ctttccggtt
    13261 tcagcgcttc gggccgcggg atggcccaca caaccaaggc gtagagcgcc accggcacaa
    13321 tcgccttcca gatccaattc ctcgtttttt cgctcacaaa tcccgtcatc atatcattgg
    13381 tctcccggca cgtacggaga tttcgcgatt tgctaccatt tcacgcaatg cgaactttgt
    13441 gcctcgccct gctgtttttc gcgcctcatg caccggccgc ggagacgtct ctcacggagc
    13501 gcgctcgcgg atacttgatc gagttgatcc ggcgggacac aaccaacccg ccgggcaacg
    13561 agactcgggt cgcggagtat ctcaaacagg tggcggatgc ctggggcatt ccgtgcgagc
    13621 tgcttggtga cgatccagcg cgattgaact tcgtggcccg gctctccggc acgggcgacg
    13681 ggcgtccgct actactcatg gcgcacactg acgtcgtgcc ggcggaccga tcgcagtgga
    13741 ccgccgatcc gttcgcgggc gagatccgcg gcggatacat ttacggccgc ggcacgcagg
    13801 acgataaggg actcctcgcc gcggagctgg ccgtgctggt cgagttgaag cggcgcgaca
    13861 tcaggctgaa gcgcgacgtc attctgttgg cggaggcgga tgaagaagcc ggttcgaccg
    13921 gcatgcgatg gctgatccgg aacgcatgga gcaagattga cgcagagttc gcgctgaatg
    13981 aggcggggtc ctcgatggac ctgccgtcgg gcgtcaggct gttccaaatc cagacctcgg
    14041 agaagatccc gacgcgggtt gtgctgtcgg cgagaggtac ggcgggtcac ggctcgcttc
    14101 cccgcgcgga caacgccgtc gttcgccttg cgcgcgccat cctccgtctg gcggacgccg
    14161 accagccggt ccacttgaat acgaccaccc ggcgctactt caaggagatt gccaggctgc
    14221 cggactatcg ctggctggtg ccgctccttc cgcgactgga gaatccctcg tcagccattg
    14281 ccgcggccaa ccagatccga gccaaagaaa cggaattgag cgccatgctg cgaactaccg
    14341 tatccccgac catgttgtcc gccggcgaga agatcaacgt catacccaac gttgccgaag
    14401 cgcagatcga cgtgcgccgg ctgccgaacg agaccgcgga ggaagtgctg gaccggttcc
    14461 ggcgcgtcct caacgactcc gccgtggagg tcagacccgc gggcgggcag gagatgccgt
    14521 cgaccgaacc gagttccctc acgacggtac tctacaaggc aatggagcga gtgctccggc
    14581 gatcacaccc gaaggcggcc gtcgtgccgt tcatgattcg gggcgccacc gacggtgcgt
    14641 tcctccggca aaaggggatg gcggtgtacg gcgtcccgat tttcgcgcgg gagggcaatg
    14701 aaagccggtc acacggaaac gacgagcggg ttgccgaggc gagcctgagc accggatcag
    14761 agttgctctg gcagattgtc gtcgcggttg cggccgagga cggagccgtt cgatagccga
    14821 cgctattgac tgcagcagga gcctgcgccg acgagagccg tcggcgaaat ctcgcgattg
    14881 tgcgcgaaga agtcctgttg cgagagattc cggtagacgt ttcccgcgat ctcggcggcg
    14941 gtcgggccgt tgacgccgcg ccctccagtc aacatgacca ccacggtcag tttgttccgc
    15001 ccgacatcgt taaaggaacc gaaccagccc atgtgcgtgc gagcgtcggt acaggtgccg
    15061 gttttgccga ggataggttc cgtcggatcg tagttcgccc ggcgtgcggt cccgtattcg
    15121 acggccgcca tcatgccggg cttgatctcc ggaatccagg actcgatatc gaggtggcgc
    15181 ttcacgcgcg ggacgaggct gagggcttcc tcggccgagc ggggatactg gaggtagtac
    15241 aacgttccgc cgttggcaat tgaggaaagc atcgccgcca gttgtaacgg tgtgagagag
    15301 atcccctcac caaagctcgt catgatgccg agattcacat tcctcggagg ctcggcagga
    15361 aagactccgg gctgctcgcc gatgatgtcc aagccggcct tctcgccaag accgaacaat
    15421 cgcgcgtagt gactgaaccg gtcgaaaccg agcttattgc ccagatgggc gaaaaaggga
    15481 ttgtcagaac gcgccagcgc ttcggtgagg gtgagccgct tgcgtccgta cactcgcatc
    15541 accgtgttgt tctcgatgat gccctcgaca agagccgcca tggcaaccgg aaccttgatg
    15601 atggagcagg gctggaagcc gcttttcagc gcaaggcgct ggttcaccat cgagaggacg
    15661 cgcccggtat tcggatcgac taccaccacc gcgccgttgt acggcccgag cgcttcgacg
    15721 gccgcgcgac gtacgaccgg atcctcgccg tcaatcacgt cgccgatggt cgaatccgca
    15781 aaggtcggct ccgaccacgg gctatacgag cggcgggtcc gccgaacccg agccctcgcg
    15841 ggctttcgcg aagtcgtcga gcggaggaca gtcggactcg agacgggctt gctcaccgag
    15901 gccgcggtcg acttcttcgc cgtcgtcgcc gccggcttgc gggtcgttgc tcgcttcttc
    15961 ttggtttggc ttgcagcgaa cccagccgtc ccgagcagaa gactgatcgt aacaatgaga
    16021 aaacggttaa gaatcccttg gcgcattaag cctgtccggc gcgcaacaga tattttccag
    16081 agcctgggtc gatgttgggt tttgaggccc gacaacttgt attgtgcggg atttccgtac
    16141 gcacagtcaa gattaacttc agttatccgg taggattccg gagccgctca ggcggcggtc
    16201 cagccgccgt cgatggtgat gaccgatccg ttgacgaaac cggcctccgg cgagctgagg
    16261 taaagagcaa ggtacgcgat ctcgtcaggc cggccgagcc tgccaacggg ctgccgctgg
    16321 ttcaattccg cgcggacctt ttccttttcg tgcttgtggt acctctccag gtacgcctcg
    16381 acgaacggcg tgtcgacggt gccgggacag atacaattca cgcgaagctg ggtggggtag
    16441 tcgacggcga gctgacgggt cgtggccacg accgctccct tggtggcgca gtacgcgtag
    16501 cggcgcttga ccccgaccag ccccgcgacc gagccaatgt tcacgatcga accttgagcg
    16561 gccagcagca gcggcagtgc gtatttcgtc acgaggtaca tgccctccac attgacgcgg
    16621 aggagccgct ggaaatccgc gaggcaagtc tcttcgatgt tgccgacgag gccgatgccg
    16681 gcgttgttca ccagggcgtc gagcgcggaa atgctggcga acatttcgtt gaccgcactc
    16741 tcgttagtga tgtcgcagat caggggagat gcgtccggca gttcggcggc gagcgtttcg
    16801 gcgcgttgcc ggtcgatatc gacgataagg acggaggcgc cggcggcggc gaaggcgcgc
    16861 gaggtcgctt cgccgatgcc gctggctcct ccggttatga gggctttgcg cccgtccaaa
    16921 cggaaaggag tgctcatcgg taggattgta ctcccggcgg cttggaccgc cgcgggatca
    16981 gcgtttgccg tcgagcttga ttccgcggct ggcgatcagc aacgcggagg agttcgcgac
    17041 ctcgccggcc tccgcgatca ccgtgacgtc agtgatgtcg gctgctcgcg gtccggcttc
    17101 ggcgttcccc ttcagtttga ggttgacgcg cgtatcgtcc acacgctcga cgacggcgag
    17161 cgtcactccc gccggaagat tctccacgga ccagcttcgc ggattcaacc gttcgacaaa
    17221 cttgccattg cgaagggctg cctgaatcgt ctttccgtct tccttgccct ggaagatcgg
    17281 ctccggctgc gagagcgaaa tcgccggagc ctgagggttc cggcggtaat agcgcacata
    17341 gtccacggtc cagtacgcgg gcaggtggag atcggactgc cggatatctc ccgcccagcc
    17401 atcgttcttg gcttcgagcg tcaggtagat ccagttcggc ttgctggcct tcatggtgcg
    17461 ggtgatccag gtttcctgac catccacaag gaaatcaaat ccttcttcgt gccagtcaca
    17521 ggcgaacaag tgccagtctg tacgagatcc tttgactgcg attcgcgctc cttcgtgctt
    17581 gtgagtgttg ttttgaccct ggtggatgtt gtgctcgacg atttcgtcgc ggtacggatc
    17641 ctcgagaatg tccacttcgg cgccgtcata gttcgggttg taggactgcc tgccccagag
    17701 ccagaaggcg ctccagtagc cgggcacgac gtcgaggttc gtgcgagctt catagtagcc
    17761 ccactggtac tcctgtttcg tgcggatgaa gccgagatga tacttcccgt ccaccttccg
    17821 cacgcggagg acgaggtggc ccctgccgtc gaggtagctg tcgtcggggt cgttgtaagc
    17881 gtttcggcgg tcgcctgtct cacacatcca gacgtcccaa ttcagtcgcg gaccgttgaa
    17941 ctcgtcatgg aaaacaagct cccatccatc tcccgggggg ccggcataca gtcggagaga
    18001 catcgcgacg aacagaacga tggcggcgca gacaggcagg aaagaaagaa gagatcggac
    18061 gtttcgcatc ggttgttcct ctttgagaac acgatcctat cactcgcgac gtacgaggcg
    18121 ggacgcccgc ttgcttttcg cgcgaccgat gttgtattcc gtaggagcac caccaccacc
    18181 tgcgagggcc ttttatgagt gaaagcatcg ggattggaat gatcggctac aagttcatgg
    18241 gcaaggctca cagcaacgcc taccgccagg tgccccagtt cttcgacacg ccagtcaagc
    18301 cgcggctgaa ggtgatctgc ggccgggacc aggcgaaggt cgagcgcatg gcgcagcgct
    18361 ggggctggga gaccgcagtc acggactggc ggcgcgtgat cgacgacccg gacgtcaaga
    18421 tcgtggacat ctgctcgccc aacaataccc atcacgatat cgccatcgcc gcgttcgagg
    18481 ccggcaagat cgtcgcctgc gagaaaccgc tcgccatgaa cggcgccgag gcgaaaacca
    18541 tggcggccgc cgcccgcaag agcgggaaga tcaacacggt gtggttcaat taccgccgcg
    18601 tgccggcgat cgcatttgcg cggcaactga tcgaggaggg ccgcatcggc cgcgtgtacc
    18661 acttccgtgc tctctacctt caggactgga cactggaccc gtccgtcccg ctggtctggc
    18721 gcatggacaa ggacgtagcg ggctcgggtg tcaccggcga cctgctgtcg catgtcgtcg
    18781 acatctccaa ctacctggcc ggcgaaatcg aggcggtcgc cggaaccagc aggattttcg
    18841 tcggcgagcg cgagcggccg gagggcggca gcgcgaaggt ggagatcgaa gatgctgcca
    18901 tcttcgtctg cgagtacgcc aacggcgcca tcggacactt cgaggccacc cgttttgcaa
    18961 acggccgcaa gaatggcaat atattcgaga tcaacggcga gcacggcagc ctgtacttca
    19021 atctcgaaga catgagccgg ttgtggtatt tcgattcgcg cgaacccaag cacgttcagg
    19081 gttggcggca gatctcggtg tgggaagaga ttcacccgta catgaagcac tggtgggttc
    19141 cgggttgcgc catcggttac gagcacacgt ttggcaatca ggcggccgac ctgctgagcg
    19201 cggtcgcggc gggaaagccg atgaagccgg atttcgatga cgggttgaag tgccaggtcg
    19261 ttctggatgc ggtcctggaa gcgtgcgaaa cggatcgctg ggtgaaggtg aaaccggtcg
    19321 gtgcttagcc ggatgcgaca agctagagga gacgtgatga agatctctct tggaagttgg
    19381 gcgttttcgt tcggtcctta cgcggacaat ccaattcctt tcgacaagac cgcccggcgc
    19441 ctgtccgaag ccggatacga cggtatcgag atttgcgggt tccccccgca catcaccctg
    19501 gaccggtacc cgacgaagaa gtcccggcgc gaggtggtgc ggttgctcgc agacctgaat
    19561 ctcggtgttt cgggctatgc cgccgacctc accagcgtga accccgtcgt agccggcaat
    19621 cgcggcgagt atctcgaggt gctccggcga aacgtggaga tgtgcgccga catcggcagc
    19681 cctgcgattc gcgttgacag cgtcgcggcg cccggatcga tcgccgactc cgattatcag
    19741 gccacattcg cgcgcgtcgc gtcactgtgg gcggaggctg ccgaaatcgc gcaaaggggc
    19801 ggcatccgga tggtctggga gttcgaaccg ggattcgtgt tcaacaagcc ttcagaagtc
    19861 gtcgcgatgc acgacagggt cgggcatccg aacttcaagg tgatgtttga tacctcccac
    19921 gcctacatgt gcggcgtggt gggggcgcgg cagcatggcg ccaaggagac gctgccaggc
    19981 ggcgttccgg aattcctgaa gaaactcgaa ggccggatcg gtcacatcca cctgatcgac
    20041 tccgacggga cacttcatgg agacgaaacg agcacgcacc ggccgttcgg cgaaggctat
    20101 atcgacttca agggcctcgc cccacagttg cttagggttc ccaacatcga gtggtggtgc
    20161 atcgaccttt gtttttgggc gaacgcctgg ggcttggtgg aacccagcct gaaattcgtg
    20221 gccaatctga tcgacgagaa gcaggcggca taggccgaac ggcggcgccg cttgccccgc
    20281 cgccgggtat cgaccatggt gtagaatcgg cggattgggc cgatgttgag ctatacgcgt
    20341 gggcctgagg cgccccttct cgagaagacc atcccgcaag tgctggcgga gaccgtcgcc
    20401 cggttcccag accgggatgc ggtcatcgtc cgccatcaga acgctaggat gacgtggctc
    20461 gaactcgccc gctcggtcga gcagacggcc cgcggacttt ccggcctcgg tcttcagccg
    20521 ggcgaccgcg tcggcgtctg gtcggcaaac tgcgtcgagt ggattctgct gcaatacgcc
    20581 agcgcccggg ccggagtggt gctggtgaat gtcaacccgg cctaccggac gcacgaacta
    20641 cgctacgttc tcgggtcttc gaagataaga gcgctgtttc tttgggagcg ggacgcgcgt
    20701 gcggactacc tccagatact tcgggattcg tgcaacggcg gtgggctacc tctcgatcac
    20761 gtcatcctgc ttgggcagga gccgtggaac cggatgctgg ctcgcggcgt cgatgttccg
    20821 ccccacgcgg cgtcgccgga agatgtcgcc aatatccagt acacctccgg aacaaccggc
    20881 gcgccgaagg gcgttctgct gacgcaccgc aatctgctga acaacggagc gctgatcgcc
    20941 ctctgtcttg gggccgatga gcacgaccgt atctgcatcc cggtaccgct ttatcactgc
    21001 ttcggctgcg tgatcggcac gatggcggca gtcaccagcg gcgcggccat gatcgtgccg
    21061 tcggcccagt ttgacgcgcg cgccaccctt caggcggttc acgccgagcg agcgaccgcc
    21121 atttacggcg tgccgacaat gttcatcgct gaactcgacc accccgagtt cgcgagtttt
    21181 gacctgacct ccctgcgcaa aggcgtgatg gccggctcgc cttgcccgat cgagatcatg
    21241 aagcgcgtgg tctccgatat gcactgcgag ggcatgacca tcgcctacgg gcagaccgaa
    21301 agctcaccag tcatcacgat gtcccgagtc gacgacagca tcgaactgcg ggtgtcgacc
    21361 gtcggccgcg cgatgccgaa caccgaggtc aaaatcgtgt ccacagggac cggtgaaacc
    21421 gtcccgctcg gcgagcaagg cgagttgtgc acgcgaggct acctcgtcat gaagggatac
    21481 gacggcgacc cggaagcgac aggcgaagcc attgacagcg aaggctggct tcacacaggg
    21541 gatctcgccg tgatgcagcc gaacggctgc tttcggatga ccgggcgctc gaaagacacg
    21601 atcattcgcg gaggcgagaa catctacccg cgcgaaatcg aggagttcct ctatacccat
    21661 ccgaaagtgg ccgacgtgac ggtggtgggt cttcccgatg cccgcctggg cgagaccgtc
    21721 tgcgcgtggg tccggctgaa gaatggctcg gcggcgacgg ccgaggagtt ccgtgagttc
    21781 tgccgcggca aaatcgccta tttcaagatc ccgcagcaca tccgctttgt ggacgcgttt
    21841 ccgatgacgg tgagcgggaa aattcaaaag ttccgcatcc gcgaaaccga gattcgggac
    21901 cgtggtttag agcaggccgc cgcgatcgag actgcctagc accacaggtt gaaacctgtg
    21961 ccacacactg tttcagtaca gcagtctcgt ccttggatcg aggcccgtct ttccgagtgg
    22021 ctgcaagatc tggcgggcgc cattcgagaa aatcacaagg tcagccggag cctggaagaa
    22081 caggaaccct tcttccatat acttctgcat caggtcggcc gtcggcgcgg gccgcccgag
    22141 cgggacgttg ttacgcttgc cagccgctac cacccgcgcg accgcttcct tcaccttcgg
    22201 atgatccgtc tgcccgccca caccgagcga ataggagaga tcgctcgtac cgatgaacag
    22261 cacctcgatg ccggggatcg cagcaatctc ctccacgttt tccaccgcct gcgcctgctc
    22321 gatgattacg atcaccataa cgttccggtt ggcgaactgg tggtaaccct ccggagcggg
    22381 ccagcggaag gccgccaggc cgggacctga accacgccgt ccctcgggcg ggtatttgca
    22441 cgccgccacc gcttgccggg caagctccgg cgtggaagtg aacggaaaca tcactccgag
    22501 gctgccgcta tcgagcaccc gcttggccgt ccacaactcg ttcaccggga cgcgcgtaaa
    22561 cggcacagcc ttcaatcccc gcgtggcgag gatcatgttg cgcgcagtct ccagtgtgat
    22621 tgggctgtgc tccatttcaa tccagagaaa atcgaatccg aggttcgcgg cattggccgc
    22681 aagctccacg ctcggaaccg tgatcgtgac gccgacaacg ggcttgccct ccttcaacgt
    22741 cttccgcacc gggttttccc aatccgaatg cgcctgttcc tgttctgacg gttgcataag
    22801 gccacagcgt atcaaactga aagacaggct gcaaacccct ggcgagcgga agaaaattgc
    22861 tcttggaatc aacgactttc gacatcacat ggacttttgt cttgacataa gtcaaacgtc
    22921 attcgtatga ttgaaacgtt cgtatgagtg agtcatacgt tagcttcaaa tgaatgcaac
    22981 aaaggaaaag atcctcgatg ccgccgaggg actcttcgcg gaacacggat ttggcgcaac
    23041 ctcactgcga cagattaccg cgcaggcggg tgtcaacctc gcggccatca actaccattt
    23101 ccgctcgaaa gacgcactga tccaggccgt attctcgcgc cggatcggtc ctatgaatga
    23161 gaagcggctg gcaatgctgg acgcgtgcga ggccgaaaac gccaatggcc ctcttccgct
    23221 ggtgcccgtg ctgcgcgcat ttgttgtccc catattcgag ctgtgccggg agcttggcga
    23281 tcggggccgg agcttcagaa ggcttatggg ccggatgtac gtcgagccag gcgacacggt
    23341 ccggaagatc ctcttcgagc agatgcaaat aatcgccggc cggttcattg cggcgttcca
    23401 gcgctccttg cctcaccttc ccatggtgga actgctgtgg cgcattcact tctgcatcgg
    23461 cgtcatctcg cacacgctgg cgggtacaaa ccacctggaa gtcgtgtcca agggtctctg
    23521 cgatcctttt gataccgagg caaccaccga gcgggttgtg gcatttctcg ttgccggaat
    23581 gcgtgcaccg ctcccggcca tgcccacccg gagctgaatc ctatgcggac attccatctg
    23641 aactggctac ccctcctgct gcttccggcg ctcgtgccgg ctgccgccca gactgccgga
    23701 accccgctcg aactaagtct caaacacgcc attgagatcg cactcgctcc ggacggcaac
    23761 gcgcgcgtac agattgcacg ccagatgatc cggcaggctc aagagcgggc aaaccaggcc
    23821 cgcgccacgc ttctgccgaa cctcgacgcc tctgtcagcc agcagaacca aacgcgtaat
    23881 ctggctgcat tcggcatccg catcgaggcg ccgatccctg gcttccggat tccggagctc
    23941 gtcgggccgt tcaacacgtt cgacgcgcgc gccaccgcta cccaaacgat cttcgacttc
    24001 ggcgccatcc gccgctacca ggcttcgcgg gcgggaatcg gcgccgccgg ggcggagaac
    24061 gacgcgacac gggaccaggt ggccctccag gtcgccgggt tgtactttgc ctccctgcga
    24121 gccgaggccc gggtgcaggc ggccgaagcg aacgtcaagc tggccgaaga gctgcttcag
    24181 ctcgccgaaa atcagaaaac cgccgggacc ggcaccggcc tcgaaatcac ccgtgcgcag
    24241 gtgcagctcg ccaacgagcg tcagcttctg ctggttgccc ggaacgagcg ccgccaggcg
    24301 aatcttcagc tcctgaaagc gatgggcctg ccgcttgtga cgcccctcct gttttcgtac
    24361 cagctcgctc gcgtcccggt ggaggaaacg acgccggaga aagccctcgc ggtcgctctg
    24421 gaatcccgcg ccgacctgaa agcgcagcgg caacgcgagg agagcgctcg cctcagctac
    24481 agcggcgcga agatggagcg cttgccctcc gtggtcggat tcgccgacta cggaacgatc
    24541 ggcaccagca tccacaacac agttccgacg cggacgtacg gcctttctgt gcgggtcccc
    24601 gtgttcgacg gtggccggcg ggacgcgcgg cgagcggaaa gcggctcgct cttcgaacag
    24661 gagaagatcc gcaccgccga tctgaaacgc cagatcgacc tcgagctgcg cctctctctc
    24721 gacagcctcc gctccgcgga agagcaggtc aacgtagcgg aggacgggct gaagcaggcc
    24781 gaggatgaac tggccagagc cagccgccgt tacaaagcag gagtggcgaa cagtctcgag
    24841 gtcaccgacg cccagacgcg tctcgcgcga gcccgcgaca atcggatctc ggctctctat
    24901 ctctacaacc aggcccgggt cgagctcgga caggcgctgg gtacgattca acagatggtc
    24961 gactgaggag ttgtggcaga catgaagaag aaaatcatcc cgatagtgat cctagcggca
    25021 gtgctggtga tcggaggcgg atacctgtac cgttggctga atcgcaatga caacggccgc
    25081 attcttatct ccggcaacat cgagctgacc gaggtcaagg ttgccttcaa gactgccgga
    25141 cggctggtgg agcgcgctta cgacgaaggc gacacagtga agaaaggcgc cgtagccgca
    25201 cgtctcgacc gcgagcagct ggaaaggcag cttgagcgcg aacgggccac actggctggc
    25261 gccgagtctg ccctggtgca gcttcgaacg gccatcgcct atcagcgcga aacgctcgac
    25321 ggcgaaactg ctgcccgtac ggcggagctc ggtcaggccg acgcccggct acgcgagctc
    25381 tcgaccggct cgcgtccgca ggaaatccag gatgcaaaag cggcggtcga agccgcgcgc
    25441 accgaacagg cccgcgcgcg caacgactgg gagcgcgcga aggtgctgtt ccagaacgac
    25501 gacatatccg cctctcagaa cgaccagttc cgtaccgcct acgaacgcgc cgccgccctc
    25561 ctccagcagg ccgagcagcg gctggcgctc gtgaccgagg gcccgcgcca ggaaaacatc
    25621 gacgccgcaa aagccggggt cgagcgggcg cgtgccaccg tacggctcgc acaagcgtcc
    25681 cgtctcgagc tcaaacgcaa ggagcaggaa ctgggggcgc gccgcgccga catcgatcgt
    25741 gccaaggccc agatcgcggt gatcgagtca cagctcaacg attccgcggt gacatccccg
    25801 atcgacggag tcgtcctcgt gaagtcggcg gaagtcgggg aaacccttgg cgcgggagcc
    25861 acggtactca ccatcggcga cctcgaccac ccgtggcttc gcggctacat caacgagacc
    25921 gatctcggcc gggtcaagct ccgctcgaaa gtcaaggtga cgaccgactc cttcccaggc
    25981 aaggtctact ggggaaccgt atcgttcatt tcgagcgaag ccgaattcac accaaagcag
    26041 atccagacgc cggaggagcg cgtgaaactc gtctaccgag tcaaggtgga cctgccgaat
    26101 ccaggccacg aactgaaatc gaacatgccg gccgacgcgg agatcctcgt cgcggacgcc
    26161 ggccgttgaa ggacccggac atggattcgg cgatcatcga agccaccgat ctcacgaggc
    26221 gtttcggcac gctgacggcg gttgaccgtc tgaatctgcg gattgccccg ggggaaatct
    26281 tcgggctcgt cggtccggac ggcgccggca aaactaccac gctgcggctg ctttgcggcc
    26341 tcctcgatcc agcggcggga agctcacgca tcgccgggca cgattccgcc cgcgaaacgg
    26401 atcaggtgaa ggacgcgatc ggatacatgg cccagcggtt cggcctttat ggcgacctca
    26461 ccgtcgaaga gaacatgaag ttctacgcgg acctgttcgg catctcccgc cccgagcggg
    26521 atgagatgac gccaaacctg ctccgcatga cgcgcatgga gccgttccgc acgcgccagg
    26581 ccggccgctt gtcgggcggc atgaagcaga aactggcgct catgtgtacg ctgctccaca
    26641 agccgcgcat cctcttcctc gacgagccga ccaacggcgt cgatccggtg tcgcgccgcg
    26701 acttctggga gatcctctac cagcttgttc gggagggact caccgtcttc gtcaccaccg
    26761 cctacctcga cgaagccgag cgctgcgacc gggttgggct catgcacaag ggccggctga
    26821 tccgctgcga tgctcccgac aaactgaaga gcacgattcc cgagccttgc tacgaagtcg
    26881 gctgcccgga cctgcgggcc gccaaggaat ttctgacgcg gcaagccggt gtggagagcg
    26941 tggagccggc tggcgcttcc ctgcaccttt ttctttcgcc ggaacgtacc agcgccgacg
    27001 ctctggccgg cgcactgcaa gccgccggcc tcggccccgc gaatttccgc cggatcgcgc
    27061 cctcgctcga ggatgcgttc attctcctga ttcatcaggc ttcgcgggag gcagcatgag
    27121 caacaacggc tgggcggtgg aggtcgaccg cctcgtgaag aggttcggca agttcgtcgc
    27181 cgtcgaccac atcacgttcc aggtccgtcg cggcgagatc ttcggatttc tcgggccgaa
    27241 tggcgcgggc aagtccacag ccattcgcat tctgtgcggc ctgttgcagc cgagcgaggg
    27301 gctggcgcgg gtgggcggca tcgatgtttc caaagacccc gaaaccatcc gccgaaacat
    27361 tggctacatg tcgcagaagt tctcgttgta cgacgatctg accgtcgaag agaacatcga
    27421 gttcttcggc ggcgtttacg gtgtgcccga ggagcagctc gccgcccgcc gcgaccacgt
    27481 tctggaaatg gccgggctca ccgagcagca cgccatgatg actcgcctcc tgcccggagg
    27541 ttggaaacag cggctggcgc tcggttgcgc tattctccac gagcctccga tcctgtttct
    27601 cgacgaaccg acttccggtg tcgatccgat cgcgcgccgc gaattctggg agctgatcta
    27661 ccgcctctcc gccgccggcc acaccgtgtt cgtgaccacc cactacatgg aggaggccga
    27721 atactgccac cgtctggcat tgatgtaccg ggggaagatc atcgccctcg gtgctcccgc
    27781 cgagttgaag cgatccgccg ccaaacccac catggaagaa gtcttcgtct cgatgatcga
    27841 agaggaagaa cggaggggcg catgaaattc cgccgctctc tcgcccggac ggtctccatc
    27901 gcccggaaag aggggttgca catcgtccgc gacccgcgca gcctgatcat ggctttggcg
    27961 gtgccgttcg tcatgctgtt gctgtttggg tacgcgctta cgctcgacgt ggatcgcgtg
    28021 ccgacgctcg tctacgatca cgaccgcagc ccgcagagcc gcgagctgat cgagcgtttc
    28081 gccggctccc gctacttccg catccttggc tccgtcgatg aatacggtgg tatcgagcgc
    28141 agcatcgacc gggatcaggc cctactcgcg gtcgtgatcc cggcggatta ctcgcgcaac
    28201 ctgctgacat caaagccggc tgaagtccag cttctgctag acgggagtga ctcgaacacc
    28261 gcctcgatcg cactcggata cgccgagtcg ctggtgcaga tgtacgcgtt cgaattgcgg
    28321 gcccgagcga ttgaccgcag gggaggccag caactgaaac cacccgtcga gacccgtctt
    28381 cgcgtctggt acaacagtga gcttcagtcg cggaactata tcgttcccgg actgatcgcg
    28441 gtgattctga tgatcatcgc cgcgttgctg acgtcgctga ccatcgcccg ggagtgggag
    28501 atgggatcga tggagcagct tctctcgact ccgttgcgtg cgcatgagct ggtcctgggt
    28561 aagatgttgg cctacttcgc cctggggttg atcgatatgg ttgcgtccat cgtcatcgga
    28621 atacaactgt tcggagtgcc tctgcggggc aaccctctgc tggttctggt cagcggcggt
    28681 gtgtttctgg tcggggcgct gttttggggc attttggtct cagcgctggc gcgctcgcag
    28741 ttactggcct accagctcgg gatggtgacc tcctacctgc cggcgttcct gctctccgga
    28801 ttcatattcg cgatcgaaag catgccggcg gtcattcagg gaatcacgta catctttccg
    28861 tcgcggtact tcatcatgat cctcaaaggg atcttcctca aaggggtagg tattcaggtt
    28921 ctttgggtgg atttcgcgtt cctgctggcc tacgcctcca tcgtctttgt agtggctaca
    28981 aagaaggtcg gccagaagct ggcatagcga tatgtggaga cgcatccggg aaatcctgaa
    29041 aaaagaattc cggcaggtat tgcgggagcc acgcatgagg gtggtgctct tcgtcccgcc
    29101 cctcttgcag ctcatcatct tcggctatgc cgtcaatctc gacgtcgagc acagccggat
    29161 cgcgtggatg gaccaggatc gcactccgga gagccgcgag ctgctggcgg ccttcgcggc
    29221 ctcggaccga ttccgggtcg tcgccacgcc tggatccgaa gaggaggtgc aggaacttct
    29281 cgatgggagc ggggtgcaga tggttgtgcg cgtcctgccg ggcttcggac gcgacgtaca
    29341 gcgcggtgag accgcttcgg tccaggtgct ggtggacggc accaactcga ataccgcttc
    29401 gatcgtcgcc ggctatgccg gtcaggtgat tgcgtccttc gggaaccgcg tgatgcacga
    29461 gcagcaggac gcccgtctgg ttggacagac agccgtcgcc ggaggaccgg tcaacaccga
    29521 cctgcccgtc ctccaggcgc gcacccgcgt ttggttcaac cccgacctcc tgagccgcaa
    29581 ctacttcgtt ccgggcgtgg tggtcaacat catctcgctg gtcacgctca tgctcaccgc
    29641 cctggcgatc gtgcgggagc gcgagatcgg caccatggag cagcttatgg tgaccccgat
    29701 ccgcccgatc gaactgatcc tcggcaagac gctgccattc gccatcgtcg gccttctcga
    29761 cctggtgctg atcaccaccg ccgccctgct cgtgttccag atcccgtttc gtggcaaccc
    29821 cctcatcctg ttgctcgcgt cggtattgtt tctgctcacg accctcggcg tcggcctgtt
    29881 catctcgacc atttctcaga cccagcagca ggcgatgatg gcctcgttct tcttcttcat
    29941 gcctacgttc atgctgaacg gtttcgcgtt tccgatccgc aacatgccgg tgccggtgca
    30001 gtacctcacc tacctgaacc ctgtgcgcta cttcctggag attacccgcg gggtcttcct
    30061 caaaggaaca ggcctggaag cgctatggcc gcagatgctg gcgctgctcg tcttcggcgt
    30121 cacgatcctg accgttagcg cgctgcgctt ccacaagcgc ctcgattgat gcgggtgctc
    30181 cccgggcgcg gtatcagccg gatgcgggat tgcggtggag gctcgcctgc ggaagaatgg
    30241 taacagatat gcgttttttg attcttctgc tcatggcgct ggcgccgcta tggccggccg
    30301 accttaatcc ggagcagcag cagcgggctc acaagctcga gacgtccctg ctcgccccgt
    30361 gctgctggtc cgagcccgtg gccacgcatc gcagcgagat ttcattgtcc atcaaggcgg
    30421 agatcgagaa gctcgtcggg gaaggcaaga ccgaccgtga gattctcgac gtgtacaaga
    30481 gccgctatgg gatgcggatt ttcgtggagc ccgaaggctc tcagtggtgg gtcatgaacg
    30541 tcgtgcccgt cgtaatgacg atacttggcg tgctcgtggt catcttcgtg atccgccgca
    30601 tgctcaggcc gattcccgca tcatcgcagt aacgacttct tccacctcgc gggcgaccgg
    30661 ctccagttcc gggtttccga atatcttcat catcgccgtg gggaggatcg tcgcaagctt
    30721 gtagatatcc ccggaacggt acacggagat ccggcaaggc aaggctgtcg acaccgcgcc
    30781 gttggcctcc agcactttct tcgcctggtg cggattacag acctcgtaaa tcatgcactc
    30841 gccggcgaag tcaacccctt tcttcttcat cgtctccttt aggtcgtgca cggcgatgac
    30901 gccgaacttg tgccgggccg cggactcctg tagcccctgg ccgatctggt cgagtgtctt
    30961 cctggaactg acttcaaaga tcatcgctat tcctcggatg gattactagt cttgggccgg
    31021 aaccgtcaga acggcgcacg gcgcatgccg cacgaccctg accgatgtag tcccgatgac
    31081 ggtgctgtcg aagaacatcc ggtgccgggc gcccagcacg aggagatcgc aaggcaactc
    31141 ggaagcagcg gaaacaataa tttcggacgc ttcaccgtcc cgcatcatct cccggacgct
    31201 gcacagcgaa cggacctcgg ccggaaccca ctcgcacaga tcggcgatcg catccttggc
    31261 gtgggattcc cgcacgtgca ggacggtgag ttccgctccg aaacagtggg ccagtttcgc
    31321 cgcgtagccg agagcagcgc gggccacgtc ggaatcgttc accgggcaaa ccacgcttcg
    31381 gatcgatggg aacggctcgg gaccgttgcc gtagggacgt accgtcagca ctgggatctt
    31441 cgcctcgcgc aagacacgct cggtgacaga ccccagcata aggcggttga agccgctgcg
    31501 accgtgggtg cccatcgcta ccagatccgc agatgtctgg tcgatggctt ggagaatgcc
    31561 gtcgaccgga agctcctcga tgacccgaac gcttacgccg gggccagcga cgccgagggt
    31621 cttgccggtg aattctttca acgagttttc tgcctcgagt ttcgcgtcct ggagttgttt
    31681 ccggatctgc tcgaggcggt cgtgcgtgaa atagggcggt ggaagaaatt ggtcggcgta
    31741 caggacatag agccgggcgc cggcgcagtt ggcaagtgcg ttcgcaaagc ggagaccgag
    31801 cgcggaggtg tcgctgaaat caacgggaca caggatgttc tgaaagcctt gcggaggcat
    31861 atctacccct ccgccattat gatgcagttt cgccgcagcg gtgacgccga ttcatcgaac
    31921 ccgggcgccg gtaactccgt atctgttata gaatgtggca ggcttaggag gtcctatggc
    31981 cgatcatgtc aagattctgg cagtattgca catcgtattc ggggtgttag gtctgctggc
    32041 agcgctcctc attctggtaa tcttcggcgg ggtggcgggc atcgtgagcg tgagcgaggc
    32101 ttctcacgac cccaacgcgt atctggcagt gccgatcctg ggtgtcgtgg gtaccatcat
    32161 cttcggcctg ttgctgctgc tctccctgcc gggcgtcctt gctggtgtgg gactcctcaa
    32221 gttccagccg tgggcgcgcg tgctcaccat cgtcctctgc gccatcaacc ttctgaacgt
    32281 cccgttcggc accgcgcttg gcatctacgg cctctgggtg ctcctcaaca gcgagaccga
    32341 gcagctcttc gccgcccgcc gagtgtgaaa cccggagact gctgaacctg ccgttctgcc
    32401 tgctcccacc tgcccgattt accgatagtc ggaaacccgc gccccggcca tactgaaaat
    32461 cgaggggtga ttcacatggc aaacgaattg gccgcgttgt cgaatgaact ggcaactgtg
    32521 gtggagcggg ccgggcaggc ggtggttgcg gtgcatgccc gcccgcggtt ttcctcgagc
    32581 ggagcattct ggcggcccgg catcatcgtc accgccgaac acaccatccg cctcgaggag
    32641 gagatcacag taacgctgcc ggacggttcg aatgttcctg cgacgttggc tggtagcgac
    32701 gcaggtactg atgtggcggt gttgcgcgtc gacggcgccg ccggctcgcc aatctcgcga
    32761 gctggcgctg tgccgtcacc cggcaatctg gccctggcca tcggccgttc acaggattcc
    32821 ggggtgaatg ccaccatggg tatcgtgagc gccgtgagcg gatcctggcg cacctggcgc
    32881 ggcgggcggc tggaccacta cattcggctg gacctaacgc tttaccccgg ctcttcggga
    32941 ggcgtggccg tcaacatcga gggtgaagcc atcggcatcg ccacctcggc gctttcccgc
    33001 attgcgggtg tggcgattcc ggcatctact gtggatcgcg tggttgacga aatcctgaag
    33061 cgcggccggg tggcgcgcgg gtatctcgga gtgggactgc aaccggtgga gttgccggat
    33121 catcaaaaag gtctgatcgt tttgtcggtc gagtccggag gtccggcgga caaggccggc
    33181 gtgctcatag gcgatattct catctcgctg ggcagacacg tggtggtcga taccgacgat
    33241 atccagaccg tgctcgaatc gcagggcgtc ggccggagcg tcgagaccga gctactgcgt
    33301 ggcggcgtcg cgaagaagct cgcaatcacc accggggaac ggtcccggag gagctgacgt
    33361 gcgcggattc ggagaaattt ccgagcggct ccggcgttcc accgcgcgag tcttcgcgtg
    33421 ggagcgagga gggggcgggt cgggcgtggt atggagcgcc gacggcctga tccttacgaa
    33481 cgcacacgtg gcaaggacgc ccgcggcgcg cgtggaattg tgggatgggc gctgtttcga
    33541 tgcgcgcgtc gtggcccgcg atccgcgccg cgatctgact gttctgcgga tttcggctga
    33601 tggcctggag gccgcaatgc ccggcgactc cgcttcgctg cggccgggcg aattggtgat
    33661 ggccattgga aacccattgg ggttcatcgg cgcactgtct accggcgtgg ttcactccat
    33721 cggcccgctg ccgggaatgg gccacgagag gtggattctc gcagacgtgc ggcttgctcc
    33781 ggggaactcc ggcgggccgc tcgccaacgc acaggggcag gttgtgggca tcaataccgc
    33841 catcgtgaac ggcttgggag tggcggcgcc ggtatcgacc gccatggatt tccttcggcg
    33901 aggcgcgcgg ccggcgcttg gcgccacgct ccggccgctg cgattcggac tgctgattct
    33961 ggaagtggac cccggcggcg cagcggtagc cgcatcgctt cgtgcgggtg atattctgct
    34021 cacctccctg ggcgaactca gcgatgccct ggactccggg ctggacgtga tgcggctgcg
    34081 ctttctgcgc gggaatcgcg aacgtgtgcg ggaggtctgc gtgcagcttc gagcgcacgc
    34141 cgaggcagcg tgatccggct cctgatcgtc gcctcatccg cagtcgtgcg cgcggggctc
    34201 gaggccctgg ccgcttcgaa ccccgggttg caggtcgtgg gctcgcttcc ggatttcacc
    34261 ggggtcgagg acgtgcatcc gaacgtcgtc cttgcggccg cgcctctgga agagattccg
    34321 cccctgcccg gcggcagcgc tgtgccgctg gttctcatca cgagtgactc gcaggcggtg
    34381 tggacgcttg aggcgctccg tctcggcgta cgcgccctgc tgccgcgcga cgcgtcgcag
    34441 gcggaaatcc ttgccgccgt cgaagccgcg gcaaccggcc tcgccgtgat cgacccacag
    34501 gatctggatg ccatgctgtc ttcggcggcg ccggttcagg ctgccggtga cgtctccgcg
    34561 ctcacggcgc gggaactgga ggtgctgcgg atgatggccg agggtgcggc aaacaagatc
    34621 atcgcctgga agctgggaat ctccgagcac acggtgaagt tccacgtggc ctcgattct
//
LOCUS       DATIFQ010000168         9134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2738987, whole genome shotgun sequence.
ACCESSION   DATIFQ010000168 DATIFQ010000000
VERSION     DATIFQ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2738987"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..666)
                     /locus_tag="VLE22_07685"
     CDS             complement(163..666)
                     /locus_tag="VLE22_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14326.1"
                     /translation="MTRLLQNSVVYSTICVLFALAIFASISAGGSVPAFGTAYLPQQS
                     YDAQLSSMPVPPPDPETPPVRFAVMPVPPPDPETPPVRLAAMPVPPPDPETPPVRLAA
                     MPVPPPDPETPPVRFAAMPVPPPDPETPPVRFAAMPVPPPDPETPPMRFAAMPVPPPD
                     PETPPVA"
     gene            complement(859..1752)
                     /locus_tag="VLE22_07690"
     CDS             complement(859..1752)
                     /locus_tag="VLE22_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB14327.1"
                     /translation="MYNAFFGFVESPFNLSPDPAFLYRSPQHEEALANLIYGVQSRKG
                     FIALTGEVGTGKTTMLECLRDYLDEEGMQFAFLFNSRLTVDQFFETIAYDLGLRCQKN
                     SKTEILFALNQLLLQEASAGRTVALIVDEAHDLDWDVLEEIRMLGNLENRGGKLLQIV
                     LAGQPELDRKLDAPRLRQLKQRIVLRFNLKPFAREETVEYIRSRMAKAGMEHQSIFPP
                     ELLAEIHVRSQGIPRVINTLCDNLLLTAFAMERKAVTADMLNEVSADMRLEWPHGELA
                     HPATPLADTPPSRSTTYTRNS"
     gene            complement(1814..2968)
                     /gene="csm5"
                     /locus_tag="VLE22_07695"
     CDS             complement(1814..2968)
                     /gene="csm5"
                     /locus_tag="VLE22_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HSB14328.1"
                     /translation="MTYRLTCLTPVLVGDGRRLSPIDYMVWKDQVNVLDQWRIFRLLA
                     KGPRLDNYLSQLKRSEKLDFASWGGFAQNFADRRILFEHPSSTAHWERAGRESLQIPT
                     FFSGPSGPYLPGSAMKGALRTGMMFSSLKEGAMASVAAQFTGDRPPRRPAEILEDREI
                     GTGGASRTRTVGASDSAPVPYSAMRIYLLRVATLVSRGKDHRELAWKTSPRGSVDGRR
                     PNDATPMFAEMAAPGTVFEGHWREIGFLSQHEIRRALNWREAPTRAAIFAAANSYAAE
                     LLRAHRQYTEWTGLSLVGQSVNQLESQLAAVRDRGDACLLSLGWGSGLLSKVAWLKTD
                     DADYRKVITQTPIYGKSIAPFLPFPKTRRVIFLENRPAVLPGWALLEVQA"
     gene            complement(2965..4002)
                     /locus_tag="VLE22_07700"
     CDS             complement(2965..4002)
                     /locus_tag="VLE22_07700"
                     /inference="COORDINATES: protein motif:HMM:NF037153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14329.1"
                     /translation="MGPALLVRLRPLGPWRIGPDSGARDRVDSVFHSDALYSALSAAF
                     VRLGLLDEWLAATFGNPGRPEVRLSSLFPSVNGSLLVVPPRNAWPPAASARVRWKGAK
                     FVPLKLVESILAGESPDERRWMVDGPSECLVPSDRPRGPFRVSMRSAAALDRLAAGVE
                     PHATACLEFNDEAGLWAVAAFADEASRERWKNRLQAAFRLLADSGFGGERSLGWGHSA
                     MPEFTDGVLPDLILPSREPAVSSLEGEPAPLPETAWWLLSLFSPAPEDAIDWNRGNYS
                     LVIRGGRVESAVRWGTPKKLTRMIVEGSVLCAEAPILGTAHDVAPDGFPHPVYRAGYA
                     LAIAIPLRVTP"
     gene            complement(4006..4842)
                     /gene="csm3"
                     /locus_tag="VLE22_07705"
     CDS             complement(4006..4842)
                     /gene="csm3"
                     /locus_tag="VLE22_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HSB14330.1"
                     /translation="MSAHTADTELRLVGKLILEGELHCETGLHIGAGKGSLEIGGADN
                     PVVKDSAGRPYVPGSSLRGRLRSLLEQSSGLAVPSELVYLSKRKGQEVRIHQSDRPDD
                     EICLLFGRSPGRMERVTGEALESGQASPARLTACDAPLEPDSISPQMRENLDDELTEV
                     KSENAIDRITSQANPRTLERVPAGARFRIRFVLDILCEEDKALPSRLVEGLRLLEDDS
                     LGGGGSRGSGRVRFGNLRLSWRGRRFYAEGQPEQELLAGADLAALQALVREDDFSGKL
                     AE"
     gene            complement(4852..5313)
                     /gene="csm2"
                     /locus_tag="VLE22_07710"
     CDS             complement(4852..5313)
                     /gene="csm2"
                     /locus_tag="VLE22_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HSB14331.1"
                     /translation="MSEVQQENRHREGRGPRDARPPREGGREGGGRPDRGRPEGPPEI
                     DLERLITDSLYLDNQAHSVVSRMRDMDSGTKSQLRRLYNAVRRACRAAENERQHQFVM
                     LRARLAYTIARHSLRSLDTLEKLLLQVTRKNDPRGYERFRDLFEAIVAYNE"
     gene            complement(5317..6792)
                     /locus_tag="VLE22_07715"
     CDS             complement(5317..6792)
                     /locus_tag="VLE22_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14332.1"
                     /translation="MSVQIFLQGKILGTEEFLLSPAPVFDSGAAVDSERLLAGRARYI
                     TLLSEVLPRALLTELGLSKILLGSSGGGQFLVVLPSEARSRAEDFLSAAANEIDKKSA
                     GHLKLVAAVTENLGDWSIVRKRLNEEMQARRGASGRPFGPDAFAPFAAIPAGGGGDYF
                     TDELGTELRTAEFAGWSPEAPGTVVTKTGKYLWSLTTGQTPDAIPFARHAAPSDDGSE
                     PADLATLARRAKGRPGWGVLRGDVDDFGVRLRRINTIEEHVQLSVMYKQFFAGEIELL
                     CSLPEFWRKVSIIFTGGDDFALYGSWDALIPLAREIQRLFHRFSEENLKDFPGPEGKT
                     LTMAITLAPEIDASLASVYEDAGHRLELAKSADKDCVHVFGRTLEWRQLAAASDLRET
                     ITRMAEELDSPQQFLAELAALYRKASVVADESTGGEARFRKPWRVYRRLNLAVGEPKA
                     REIQKLRNQLTNEMLAKSFAQVKLRPGGRVALDWARLLTEV"
     gene            complement(6904..7908)
                     /locus_tag="VLE22_07720"
     CDS             complement(6904..7908)
                     /locus_tag="VLE22_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB14333.1"
                     /translation="MKLSRNWKMMGAAAALCGGALSLPAQERPVFRVKVDMVVLTFTI
                     TDSRGRYITGLKPGDFRILEDGIVQKVSTFGEGSKPAVQVLPDGNTRPIIVPEGAAEP
                     EAALERRPDAFVGTNVFVLFDTSNYMYRGFVYASDAIADFVRGLDRADSVAVYTFSRN
                     LSRAASLTQDRNNAILGLRKAVAGDDAALYNALLLCLRDAAKVPGRKVVIVFSNGPDN
                     ASMVAPDDVRTVAEDEGIPIYVISTSEVNKDPISSAVFRRISNRTGGKAYFAKTWQKQ
                     VEAFESIREDLGNSYTVTYYPAPNPNEGFRKISVEIVSDVGKKYRIRARPGYRPRPSS
                     "
     gene            complement(7945..>9134)
                     /locus_tag="VLE22_07725"
     CDS             complement(7945..>9134)
                     /locus_tag="VLE22_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14334.1"
                     /translation="ASRTAEIESPRASLTATEKNAFAFGYWTERHIENQRKLNLRATL
                     ERVGTDSGKLRETRGLLASFLRDTLVGLVYIHYTPPGAQLLVTNPVFVRSHDFIGIQG
                     ANHTWRSTDVYGSGWPSSAGGKLVGSLTGLPYALAEAEQNFMIPTREQALIWGDLVPQ
                     MILSAKIPRWWNVSPAQLHWVGLHMRYAESLVAESAFDAGRRAVTLETLARMATPARV
                     GKVAGLIERGDVGAALAAITPAELFMVAKEALARDKDSSGPLAAAIRSATAESPDHIN
                     YAFVSRAFGTPKPTLTNSYQPELLYLRTFPTLMGYSSRIMAESWESNALYWAALADEV
                     HLPPSQLNFLIPEWTQQTVERIFATHLEDWPAVLRSLRLIGDDIRLKARQQIAGEQKA
                     SLE"
BASE COUNT         1623 a         2889 c         2948 g         1674 t
ORIGIN      
        1 gaagcacaat tccgagccag atggggaccg accaaaaata agtcatacaa gtccggctgt
       61 gtgcagcttg accggatctt atattctatc cgtgcatcca tcctctacgg gccggtacat
      121 cgccagtgcc ggcccgtgct cgtccgagag cggtcgtgcg gcttacgcga ccggcggcgt
      181 ttcggggtcc ggtggaggca ccggcatggc ggcgaagcgc attggcgggg tctcggggtc
      241 tggtggaggc accggcatgg cagcgaagcg gaccggcggc gtttcggggt ccggtggcgg
      301 taccggcatg gcggcgaagc gcaccggcgg ggtctcgggg tcaggcggag gtaccggcat
      361 ggcggcgagg cggaccggcg gcgtttcggg gtccggtgga ggtactggca tggcggcgag
      421 gcggactggc ggggtctccg ggtcgggcgg aggcaccggc atgacggcga agcgcaccgg
      481 cggggtctcg gggtcgggcg gaggcaccgg catcgagctc aactgcgcat cgtacgattg
      541 ctgtggcagg taggcggtac cgaaggccgg aacggagccg ccggccgaaa tgctcgcgaa
      601 gatcgccaac gcgaacagga cacagatagt cgaatacact acggagtttt gcaatagtct
      661 tgtcatttgt aatgtccctc gggggtggtg agttcttcac gtcgccggtc agtccggtaa
      721 atccctggat cgatgtcgtg aattaccccg attgtagtct agggattaac tcctactaga
      781 atcagataaa tccgtaaggt attcgtccat gggggacttt ggccccctcg cacgagggcc
      841 tctaccggaa gaaatgcact agctgtttcg ggtgtaggtc gtggacctgg acggcggggt
      901 gtccgcaaga ggagtggcgg ggtgggcgag ttcgccatgc ggccattcca ggcgcatatc
      961 ggcgctcact tcgttcagca tgtccgccgt caccgccttt cgttccatcg cgaaagcggt
     1021 cagcaggagg ttgtcgcaga gagtgttgat aacccgggga ataccctggg agcgcacgtg
     1081 gatctccgcg agcagttccg gcgggaagat cgactggtgc tccatcccgg ccttggccat
     1141 gcgcgaacgg atgtactcga ccgtctcctc ccgtgcgaac ggcttcaaat tgaaacggag
     1201 aacaatgcgt tgtttgagct gccgcagccg tggcgcgtcc agtttccgat ccagttccgg
     1261 ctggcccgca agcacgatct gaagcagttt cccaccccgg ttctcgaggt tcccgagcat
     1321 gcgaatctcc tcgaggacgt cccagtccag gtcgtgcgcc tcgtccacga tcagagcgac
     1381 ggttcgaccg gctgacgctt cctggagcag caactggttc agggcgaaca atatctcggt
     1441 ttttgagttc ttctggcagc gcagaccgag atcgtaggcg atcgtctcga aaaactggtc
     1501 cacggtcaag cgcgagttga acagaaaggc gaactgcatg ccctcttcgt ccagatagtc
     1561 ccgaaggcac tccagcatgg tcgtcttgcc ggtgcctacc tcgccggtca gggcgatgaa
     1621 gccctttcgg ctctgcacgc cgtagatcag gttggcgagc gcctcctcgt gctgcgggct
     1681 gcggtacagg aaggcgggat ccggactcag gttgaagggg ctctccacga atccaaagaa
     1741 agcgttgtac ataggaccag gcccgcgaaa cgggcgaagg caagcgctaa ctcttgcatc
     1801 ttaacatgct gtgtcaggct tgcacttcca ggagcgccca accgggaagc acggcgggac
     1861 ggttttcgag gaaaatcacg cggcgggtct tcgggaaggg aaggaatggc gcgatactct
     1921 tcccataaat cggagtctgg gtgatcacct tccgatagtc ggcatcgtcc gtcttcagcc
     1981 aggcgacctt cgacagcaga ccgctgcccc agccgaggga gagcaggcag gcgtctcccc
     2041 tgtcgcgcac ggccgcaagt tgcgattcca gttggttcac ggactggccg acaagggaaa
     2101 gccctgtcca ctcggtatat tgccggtgcg ctctgagcaa ctcggcggcg tacgagttgg
     2161 ccgcggcgaa aatggctgca cgggtgggag cctcccgcca gttcagcgcc cgccggatct
     2221 cgtgttggga gaggaaaccg atctcccgcc agtgtccctc aaatacagtt ccgggtgcgg
     2281 ccatctccgc gaacatcggc gttgcgtcgt ttgggcgccg gccgtccacg gagccgcggg
     2341 gactcgtctt ccaggcgagt tcacggtggt cctttccgcg ggagacgagc gtagccacac
     2401 gaagcaggta gatcctcatc gcggaatacg gcacgggagc ggaatcggaa gcgccgaccg
     2461 tcctggtccg cgacgcgcct ccggtcccga tttcgcgatc ctccagaatc tcggccggcc
     2521 gccgaggcgg gcggtcgcca gtgaactggg cggccacgga tgccattgcg ccttccttga
     2581 gactgctgaa catcatgccg gtgcgcaaag ctcccttcat cgcggatccc gggaggtaag
     2641 gtccggaggg acccgagaag aaggtcggaa tctgaaggct ctcgcgcccg gcgcgctccc
     2701 aatgggccgt cgaggaagga tgctcgaaga gaatgcggcg gtcggcgaag ttctgggcaa
     2761 agccacccca ggaggcgaag tccagctttt cggagcgttt cagttgcgag aggtagttgt
     2821 ccagccgggg acccttcgca agcaggcgaa aaatgcgcca ctgatcgagg acgttcacct
     2881 ggtccttcca gaccatgtag tcgatcggcg acaggcggcg gccgtcgccg acgagcaccg
     2941 gagtgaggca agtcaggcgg tatgtcatgg cgtcacccgc agcgggattg cgatcgccag
     3001 ggcgtaaccg gcccggtaca cgggatgcgg gaagccgtcc ggcgccacat catgagctgt
     3061 gcccaggatg ggggcctcgg cacacaatac cgagccctcg acgatcatcc gggtcagttt
     3121 cttgggggtg ccccaacgca cggcactctc cacccggccg ccccggatca cgagcgagta
     3181 gttgccgcgg ttccagtcga ttgcatcttc cggggccggg ctgaacagcg acagcaacca
     3241 ccacgctgtc tcggggagcg gcgcgggttc tccctccaac gatgacacgg ctggttcccg
     3301 cgacggcaga atcaggtccg gcaggacgcc gtcggtgaat tccggcatgg ccgagtgtcc
     3361 ccacccgagg cttctctcgc cgccgaaccc ggagtcggcg agcaggcgga acgccgcctg
     3421 gaggcggttc ttccagcgct cccttgacgc ctcatccgcg aacgcggcca cggcccacaa
     3481 gccggcttcg tcgttgaact ccagacacgc cgtcgcgtgc ggttcgacgc cggcggccag
     3541 ccggtcgagc gcggccgcgg accgcatgga cacacggaag ggcccgcggg ggcggtcgga
     3601 gggcacaaga cactcgctgg gtccgtcgac catccagcgc cgttcatccg gactctcgcc
     3661 ggcgagaatg gactcgacca gtttcagcgg cacgaacttc gcgcccttcc accgcactct
     3721 agcggaagcc gcgggaggcc aggcatttcg cgggggcacg acgaggaggc tgccgttgac
     3781 gctcgggaac aaggaactca ggcgcacctc gggccggccg ggatttccaa acgtagccgc
     3841 cagccactcg tcgagaaggc cgaggcggac gaatgcagcg gacaaggcgg agtaaagtgc
     3901 gtcgctatga aataccgagt cgacgcggtc gcgggcgccg gagtccggcc cgatacgcca
     3961 cggcccgaga ggccgcaggc gaacaagtag cgcggggccc atggtttact cggccagttt
     4021 ccctgagaaa tcgtcctcac gaacgagagc ttgcagggct gccaggtccg cgcccgccag
     4081 cagttcctgt tccggctgtc cttcggcata aaagcgccgc ccgcgccatg aaagccgtaa
     4141 attcccgaag cggacgcggc cgcttccgcg ggaaccgccg ccgccaaggc tatcgtcctc
     4201 gaggagacgt aagccctcga cgaggcgcga tggcagggcc ttgtcttcct cgcagaggat
     4261 gtcaagcacg aagcggattc ggaagcgcgc tccggccggc acacgttcga gggtgcgcgg
     4321 gttggcttgc gacgtgatgc ggtcaatcgc gttctcgctc ttcacctcgg tcagttcgtc
     4381 atccaggttc tcgcgcattt gcggcgagat gctgtccggt tcgagcggag cgtcgcacgc
     4441 ggtaaggcgc gcagggctgg cctggccgct ctcgagagcc tcgcctgtca cgcgttccat
     4501 ccgtcccggg ctccgcccga acaggagaca gatctcgtcg tccggacggt cgctctggtg
     4561 gatccgcacc tcctggcctt ttcgcttgga gagataaacc agctccgacg gtacggcgag
     4621 gccggatgac tgttccagaa gcgaccgcag ccgcccgcgg agagaactgc ccggcacgta
     4681 gggccggccg gcggagtcct tgaccaccgg gttgtcggcg ccgccgattt ccagggagcc
     4741 tttgcccgcg ccgatgtgca ggccggtctc gcaatgcagt tcgccttcca gtatgagttt
     4801 tcctaccaat ctgagttccg tgtcggcagt gtgtgcgctc atgctccctc gctattcgtt
     4861 gtatgccacg atcgcttcga acaggtcgcg aaatcgctcg tagccccgcg ggtcgttctt
     4921 tcgggtgact tgcagcagga gtttctccag cgtgtcgagg ctgcgcagcg aatggcgcgc
     4981 gatggtgtac gcgagcctcg cgcggagcat cacgaactgg tgctgccgct cgttctccgc
     5041 ggcgcggcag gcgcggcgca ccgcgttata gagccgacgc aactggctct tcgtgcccga
     5101 gtccatgtcg cgcatgcggc tgacaacgct gtgggcctgg ttgtcaaggt acaggctgtc
     5161 ggtgatcagc cgctccaggt cgatttccgg agggccctcg gggcggcccc tgtcgggacg
     5221 gccccctccc tccctgccgc cttctcgcgg cggccgcgcg tctcgtggcc cgcggccttc
     5281 ccggtgtctg ttttcctgtt gcacttcgct catggattac acctctgtca gcagtctcgc
     5341 ccagtcgagc gccacgcggc cgccgggccg caacttgact tgggcgaaac tcttcgccag
     5401 catctcgttg gtcaactgat tccgcaactt ctggatttcg cgcgctttcg gctcgccgac
     5461 cgctaggttc agccgccggt agacacgcca gggcttgcgg aagcgggctt ccccgccggt
     5521 ggactcgtcc gcgactactg acgccttacg gtatagggct gccaattcgg cgagaaactg
     5581 ctgcggggaa tccagttcct cagccatgcg cgtgatcgtc tcacgcaggt ccgatgcggc
     5641 cgcgagctgc cgccactcga gcgtccggcc gaagacgtga acgcagtctt tgtcggcgga
     5701 cttggccagt tccagtctgt ggccggcatc ttcgtagacg gaggccaggc tggcatcgat
     5761 ctccggcgcc agggtgatcg ccatcgtgag cgttttgcct tccgggccgg ggaaatcctt
     5821 caggttctct tcgctgaacc ggtggaacag gcgttggatc tcgcgggcca gcgggatgag
     5881 agcgtcccat gagccgtata gagcgaagtc gtctccaccg gtaaaaataa tgctgacttt
     5941 ccgccagaac tcgggcagcg aacacagcaa ctcgatctcg ccggcgaaga actgcttata
     6001 catcaccgag agctgcacgt gctcttcgat tgtgttgata cgacgcagcc gcacgccaaa
     6061 gtcgtcgacg tccccgcgca gcactcccca ccccggccgg cctttagccc ggcgcgccag
     6121 tgtcgcaaga tccgccggct cggagccgtc gtccgacgga gccgcgtgac gggcgaatgg
     6181 gattgcatcg ggggtctggc cggttgtcag actccacaag tatttgccag tctttgtaac
     6241 gacggttccg ggagcctcgg gagaccagcc ggcgaactcc gcggtccgaa gttccgtgcc
     6301 gagctcgtct gtaaagtagt ctccgcctcc gcccgcgggg attgccgcga acggtgcgaa
     6361 ggcgtctggt ccgaacggcc ggcccgatgc gccgcggcgg gcctgcatct cttcgttgag
     6421 gcgttttcgc acgatgctcc agtcgcccag gttctccgtg acggccgcca ccagcttgag
     6481 atgaccggcg cttttcttgt cgatttcatt cgccgccgcc gacaagaaat cctcggccct
     6541 cgaacgagcc tcggaaggaa ggaccacgag gaactggcca cccccgcttg acccaagaag
     6601 gatcttcgac agtccgagct ccgtaagcag cgcccgggga agcacttcgc tgagcagcgt
     6661 gatgtaacgg gctcgaccgg caagaaggcg ctcactgtcg accgctgcgc ccgaatcgaa
     6721 aacaggcgca ggcgacagca aaaactcctc tgtaccgaga atcttaccct gaagaaagat
     6781 ttggaccgac atccggaaag agtcccgctg aaagccgtca gtctaccaca gcttcacgac
     6841 cgccgattgc ctctctgtcc acagagagcg tctcgaccgc ccacgccgat gtcgcggccg
     6901 gtcttaggaa ctcggtctcg gccggtatcc gggccgggcg cggatccgat acttcttgcc
     6961 cacgtcggag acgatctcga ccgagatctt ccggaaaccc tcgttcggat tcggcgcggg
     7021 atagtaggtg acggtgtagg agttgcccag atcctcgcgg atggactcga atgcttccac
     7081 ctgtttttgc caggtcttcg cgaagtacgc tttgccgccg gtgcggttgc tgatgcggcg
     7141 gaaaacggcg ctcgagatcg ggtccttatt cacctcgctg gtcgaaatga cgtagatcgg
     7201 aatgccctca tcctcggcga cggtcctcac gtcgtctgga gcgaccatgc tggcgttgtc
     7261 cgggccgttg gaaaagacga tgacgacttt gcggcccggc actttggctg cgtctcgcag
     7321 gcagagaagc aacgcgttgt agagcgccgc gtcgtcgccg gccaccgctt tccggagccc
     7381 gagaatcgca ttgttgcgat cctgcgtcag actggcggcg cgcgacaggt tccggctgaa
     7441 ggtgtacacg gcgaccgagt ccgcacggtc caggccacgg acgaaatcgg cgatcgcatc
     7501 ggaagcgtac acaaagccgc gatacatata gttgctggta tcgaagagca cgaagacgtt
     7561 ggtgccgacg aacgcatcgg gacgacgttc aagggcggcc tccggttcag cggcgccctc
     7621 cgggacgatg atcgggcgag tgttgccgtc aggcagcacc tgaacagccg gcttgctgcc
     7681 ttcgccgaaa gttgacacct tctggacgat cccgtcttcc aaaattcgga aatcgcccgg
     7741 cttcagtccg gtgatgtaac gtcctcggct gtcggtgatc gtgaaggtga gcacgaccat
     7801 gtcgaccttc actcgaaaca ccggcctctc ctgtgccggc agggacagcg cgccgccgca
     7861 cagagccgcc gcggccccca tcattttcca atttcggctg agtttcacgc gatgctcctc
     7921 ccctccgtga gttccgtgcc cgaactattc cagcgacgct ttctgctcac cggctatttg
     7981 ctgtctggct ttaagccgaa tatcgtcacc gatgagacgt aacgatctga gaaccgccgg
     8041 ccaatcctcg aggtgcgtgg caaagatccg ctccacggtt tgttgggtcc actccgggat
     8101 caggaagttg agttgcgatg gaggcagatg aacctcgtcg gcgagcgccg cccagtacag
     8161 agcgttcgat tcccagcttt ccgccatgat ccggctcgag tatcccatca gcgtggggaa
     8221 cgtgcgcaga tacagcaatt ccggctggta ggaattcgtc agcgtgggtt tcggcgtacc
     8281 gaacgcgcgg gagacgaacg cgtagttgat gtggtccggc gactcagctg tagcgctccg
     8341 aatcgctgcc gccagcggtc cggaggaatc cttgtcccgg gcgagagctt ctttcgccac
     8401 catgaacagc tccgccggcg tgatagcagc cagcgccgct cccacgtctc ccctctcgat
     8461 cagcccggcg accttgccga cccgcgccgg cgtcgccatc cgcgccagcg tctccagggt
     8521 cacggcgcgc cgtccggcgt caaacgccga ttccgcgacc agggactccg catagcgcat
     8581 gtgcagccca acccagtgca attgggcggg gctcacgttc caccagcgcg ggatcttcgc
     8641 actcaggatc atctgcggaa cgaggtcgcc ccagatgagc gcctgttcgc gggtgggaat
     8701 catgaaattc tgctccgctt ccgccagcgc gtacggcagg cccgtcagtg aaccgaccag
     8761 ctttccgccc gcgctcgatg gccagccgct gccatacaca tctgtggagc gccacgtgtg
     8821 gttcgcgccc tggatgccga tgaaatcgtg gcttcgtaca aacaccggat tggtcaccag
     8881 caactgcgcc ccaggtggag tgtagtggat gtagacgagt cccaccaggg tgtcgcgaag
     8941 gaacgaggct agcagtcctc gggtctcccg caactttcca ctgtcggtcc ccacccgctc
     9001 cagcgtggca cgcaagttca gcttgcgctg gttctcgatg tgccgctctg tccagtagcc
     9061 gaacgcgaag gcattctttt ccgtggcggt gagcgaagcc cgcggcgatt cgatctcggc
     9121 ggtgcgcgac gcaa
//
LOCUS       DATIFQ010000169         3550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_249360, whole genome shotgun sequence.
ACCESSION   DATIFQ010000169 DATIFQ010000000
VERSION     DATIFQ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249360"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VLE22_07730"
     CDS             complement(<1..399)
                     /locus_tag="VLE22_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14335.1"
                     /translation="MMRRLVMCLLLAAFCRADDATLVIRTPMSPPEWALLERELLRAN
                     SDACERFAAQYLDERGYLLHTPRWGTLDGPDDAIETFYNWTLLHALGARDSVLTLFEK
                     AYEGHLNQYKELRTTKTELAKDGAYYKEFIT"
     gene            432..1352
                     /locus_tag="VLE22_07735"
     CDS             432..1352
                     /locus_tag="VLE22_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB14336.1"
                     /translation="MPQNRLSRRGFLLASASTGAAFAAGSPVIDTHMHVWSLDMARYP
                     ISPPEPGIPPQTIDGSAERLIEEMRRDGVDLAVLVQPRQYGWDNRLIADSVRRYPRLF
                     VGHGLIDPHDPKNAAVLEREVRENGLVGMRLSPIYHPKETWLNAPSNYALWRKAQELG
                     AVFNVFIAAPQIPQLEDMVRRFPKVKVVVDHLGRPEILNRPPWVENDSLLRLARYPNV
                     WVKFTELYTASKNKVYPYRDVQPFGRRVYDAFGPKRLLFGTGMVGATRRIPLADELRL
                     IREDIPYFTDGDKPWILGRNALSIWKWPKA"
     gene            3053..3382
                     /locus_tag="VLE22_07740"
                     /pseudo
     CDS             3053..3382
                     /locus_tag="VLE22_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          693 a         1114 c         1055 g          688 t
ORIGIN      
        1 cgtgatgaac tccttgtaat aggcgccgtc tttggcgagc tcggtcttgg tggtccgaag
       61 ttctttgtac tggttcaggt gcccttcgta ggctttctcg aagagggtga ggaccgaatc
      121 ccgtgcgcca agcgcgtgga gcagcgtcca gttgtagaac gtctcaatcg cgtcgtccgg
      181 tccgtcgagt gtgccccagc ggggcgtgtg cagcaggtag ccgcgctcgt ccagatattg
      241 cgccgcgaac cgctcgcatg catccgagtt ggcgcgcagc agctcccgct cgagcaacgc
      301 ccactccggc gggctcatcg gtgtccgaat aaccaaggtt gcgtcgtccg cccggcagaa
      361 cgccgccagg agcaggcaca tcaccagccg tcgcatcatt cccctatcat attcggagag
      421 tgcaatccgc tatgccacaa aacaggcttt cccgcagagg attcttgctg gcctccgcca
      481 gcaccggagc ggccttcgcg gccggttccc ccgtgatcga cacccacatg cacgtctgga
      541 gcctggacat ggcccgctac ccgatcagcc cgcccgagcc cggcatcccc ccacagacga
      601 tagatggcag tgccgagcgg ctcatcgagg agatgcgccg cgacggcgtg gacctggcgg
      661 ttctggtgca gccccgccag tacgggtggg acaaccgcct catcgccgat tccgtgcgcc
      721 ggtatccgcg tctgttcgtc ggtcacgggc tgatcgatcc acacgacccg aaaaacgccg
      781 ccgtgctcga acgcgaagtc cgcgagaacg gactcgtggg aatgcgtctc agcccgatct
      841 atcatccgaa ggaaacctgg ttgaacgcac cctcgaacta cgcgctgtgg cgcaaggcac
      901 aggagttggg cgcggtgttc aacgtcttca ttgccgcgcc gcagattcca cagcttgagg
      961 acatggtccg gagattcccg aaggtgaagg tggttgtgga tcacctcggc cggccggaga
     1021 tcctcaaccg cccgccgtgg gtggagaacg actcgctgtt gcggctggcg cgctacccga
     1081 acgtctgggt caagttcacg gagctgtaca cggcatcgaa gaacaaggtg tacccgtacc
     1141 gcgacgtgca gcccttcggc cgccgcgtct atgacgcttt cggcccgaag cgcttgttgt
     1201 tcggcaccgg catggtcggc gcgacgcgcc gcatcccgct cgcggacgag ctacgcctca
     1261 tccgcgagga catcccctac ttcactgacg gcgacaagcc ctggatcctc ggccgcaacg
     1321 cgctttcgat ctggaagtgg ccgaaggcgt gatccgtttc cgcacgcaga gccgcagccg
     1381 aaggaagggg acgctcatct ggctctagcc gcgccggctc acgccgctcc tcgccctgct
     1441 gccggccgcc aggaaactga caaccgttta ccgctggacg cgcattctcg gaggtaaagg
     1501 ttgatcaacg tctgatagcg gattcctgtt tcctccgcaa ggtgctcgaa ataacggatt
     1561 gtatcttggt caaggcggat tgtcacctgt cgtttcagcc gtcgcgcata gggattgcgt
     1621 ttgcctttgg aaaagtcgta atgctttctc attgagccca cctggcctcg tacccttgcc
     1681 gctctttctg attcgcgcga cgagccgata tgagtcgaat tacattcccc cgtcgtcggt
     1741 agcaatgaca gaccaccagc acccgcaaca gaccactaag cccgaggagt acgaatcgtt
     1801 cctcgtgctc tgaatggtct ggatcggtga tcaccactgc attctcgtcg aagaacactg
     1861 tccgggcttc ttcgaaggaa accccgtgct tccggatgtt ctgcgcgttt ttccgatcat
     1921 cccactcgaa gcggagcacg tccaccagga ccagtgtaat tacaatgtaa ggagagtgca
     1981 atccctgtag actgtggctt gcgtcgccaa acctgccgtg ccggcaggcg agtgctgacg
     2041 tttcgacgtt aagatgtcgt agccaaaatc gaacgtcttg ccggcagtcc ccggccgcct
     2101 gcctccgaaa agctctccgg cgaggagaag taccgacttc gccaaggcga ctatcgcatc
     2161 ctgtatctaa ttgaaggcgc cagcttgtcc gtcacgatcg tgaagatcgg ccaccggcgg
     2221 gaggtctacc gcacttgaac ctgcgggcct agtagtcggc gggtcgtcac gtgccacttt
     2281 ggccgccaac cctcccctgc cggcaggcag ttatcgaacc gcgcccgaac ggagccgtcg
     2341 cctatcctcc tcaagtggtg ccggctcacg cggctcgttc tggttcgcac gacgttcatc
     2401 cgactggaag cggagcaagt acaaaatcag tgtaattaca acgtaaggag aatgcagccc
     2461 cagtagccgt ggcgtgggct gccaggccgg ccgtaccggc agtcctgccg gcagtcgcca
     2521 gctcgacctt gaagcactgc gacgttatga tgccattgat gcccaccgcg aaacgcacga
     2581 cagtgtattt cgatccgacc tagtcagctc cgacggcggc ctgattctgg cgcgtgagtt
     2641 ggaggagcgt ttgtgcttcc gcgaactcat cgcccagaca cttggccgac ccgcgagaga
     2701 cgaacaccgt tgcccctccc cgacctgctg cggcagtcgg tgtatagccg cttggcgggt
     2761 tgcgaggacg cgaacgacgc cgagcggctc tcacaagatc cggcgttctg gctcatcaga
     2821 tcggagtaga tctgggagcg tggggcggca ctgacttctc ggttgcaatc attcgagacg
     2881 gacttgctta ctcaagaagg aaacctcgct ggcctggctt cgatcaaccg agagctgatc
     2941 accaaggcgg aagccatcga gtcaccgcag cgggtggtgc tgctggtggt actgaacatg
     3001 gacagcacgg agattccggg tcctcggcga gcagtagcag agggcctaca acggccactt
     3061 cgggtcgagt tgctaccacc cgctcttgac tgttcagccg cgaggcgact gtccggcggc
     3121 aaagctgcgc gccggtaacg tccacagtga cgacgactgg gaagaactgc tgccgcccga
     3181 gatcgagcag caacagaagc tgggcaaaga ggtggtcttt cgtgccgacg ctgccttcgc
     3241 caagccggag atctatgagg cgctggagaa gcggggcgtg cagtacgccg tccgcatctc
     3301 gtccaatgac agcctagagc gggacatcgc cgagttgcta acgcagtctg tgggtcgaac
     3361 gagccacaag ccagtggtct gatacaaggg ctttctgtac caagcggcta gttggaacac
     3421 ggcacggcgg atccttttta cagctgtttt accgattccc gacaacttcc acttcccatc
     3481 gacgttaatc ttagacgtgg atggcaaacg ccctccacag aaaggaagga aacttgagaa
     3541 tcgttcccct
//
LOCUS       DATIFQ010000170         2074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1781650, whole genome shotgun sequence.
ACCESSION   DATIFQ010000170 DATIFQ010000000
VERSION     DATIFQ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781650"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1355)
                     /locus_tag="VLE22_07745"
     CDS             complement(join(136..1064,1064..1355))
                     /locus_tag="VLE22_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HSB14337.1"
                     /translation="MNTSKRYSPEVQERAVRLVFEHEGEHESQWAAIGSIAAKIGCTA
                     ETLRKWVRQAERDRGRRAGLTTTERDRLKELERENRELKRANEILRKASAFFAPGGAR
                     PPTEVMVEFIDRHRDVYGVESICAQLPIAPSQYYEHKVREADPARLPPRLARDRVLAQ
                     EIRRVYEENFHVYGARKVWRQLGREGEVVARCTVERLMKSLGLQGVVRGRKCRTTIAD
                     DAAERPLDRVNRQFRASRPNQLWVADFTYVATWAGFVYVAFVIDVFARRIIGWRVARS
                     MQTDLVLDALEQALWARSGIKGVVHHSDRGGQYLSIRYSERLAEAGVDPSVGSVGDSY
                     DNALAETIIGLYKTEVIHRRGPWRHVDAVEYATLEWVDWFNHRRLLEPIGNVPPAELE
                     ASYYQSTGQLPLAA"
     misc_feature    complement(961..1077)
                     /locus_tag="VLE22_07745"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(1407..1505)
                     /locus_tag="VLE22_07750"
                     /pseudo
     CDS             complement(1407..1505)
                     /locus_tag="VLE22_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1696..>2074)
                     /locus_tag="VLE22_07755"
     CDS             complement(1696..>2074)
                     /locus_tag="VLE22_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14338.1"
                     /translation="FPGRRLHVLEDLPKGVALQWASRRRPDFWWNLRSEDGSLDQALI
                     GALLDRFQSIRARTLALRFSDDPFATAAATGRILSLFMNCDSSSVQIAPRDVESQTIG
                     HFGFFRSRFRASLWPRVTDWLQS"
BASE COUNT          396 a          700 c          604 g          374 t
ORIGIN      
        1 cttcggaatg agcttcagcg gcgccggcgc ggtgcgcacg ccctggctgc agcaggggca
       61 ggcgtacttc acccgctcgt ggcggatcac gcgcactgaa ccgccccgga ttttccgggg
      121 agctccgggt ttgagtcagg ctgccagcgg cagctgaccc gttgactgat agtacgacgc
      181 ctcgagttcc gccggcggca cgttgccgat cggctcgagc agccggcggt gattgaacca
      241 gtcgacccat tccagagtcg cgtactcgac ggcgtcgaca tgccgccacg gcccacggcg
      301 gtgaatgacc tccgtcttgt agagcccgat gatcgtctcg gccagcgcgt tgtcgtaaga
      361 gtccccgaca ctgccgaccg acggatcgac gccggcctcg gccaagcgct cggaatagcg
      421 aatcgacaaa tattgtccgc ccctgtccga atggtgcacg acacccttga ttccggatcg
      481 cgcccacaga gcttgttcca gcgcgtccag gaccaggtcc gtctgcatcg agcgggccac
      541 ccgccaaccg atgatgcggc gagcgaacac gtcgatgaca aacgcgacgt agacgaagcc
      601 ggcccacgtc gcgacgtagg tgaagtcggc cacccacaac tgattcggcc ggctcgcccg
      661 gaactgccgg ttgacccgat cgagcggtcg ctcggcagcg tcgtcggcga tcgtggtccg
      721 gcacttcctc ccacggacga cgccctgaag gcccagcgac ttcatcaggc gctcgaccgt
      781 acagcgggcc acgacctcgc cttcgcggcc gagctggcgc cacaccttgc gtgccccata
      841 gacgtggaag ttctcttcgt acacgcggcg aatctcctgc gccagcacac gatcgcgtgc
      901 aagccgcggc ggcaaccgcg ccgggtccgc ctcccgcacc ttgtgttcgt agtactgcga
      961 cggggcgatc ggcaactgcg cgcagatcga ctcgaccccg tatacgtcgc gatgccggtc
     1021 gatgaactcg accatcactt cggtcggcgg tcgagctccg cctgggcgaa aaaagccgac
     1081 gccttacgca gaatctcatt ggcccgcttc aactcccggt tctcgcgctc cagttccttg
     1141 agccggtcac gttcggtggt ggtcagcccc gcacgtcgac ctcgatcacg ctcggcttgc
     1201 cggacccatt tgcgcagcgt ctccgccgtg cagccaatct tcgccgcaat cgagccgatc
     1261 gccgcccact gcgattcatg ctcgccctcg tgctcgaaga ccagccggac cgcgcgctct
     1321 tggacctccg gggaatacct ctttgacgtg ttcatgactc catcctctca agcaatggag
     1381 tctccgggaa tcccggggcg gttcaagatg ccaacgcggc gaatcaactc cacgaccggg
     1441 agaccggcct cggcttgctt caacgccgct acgatctgtt cgacgctaaa ccgcttccga
     1501 ttcacggcaa gacctccgtc ccataagggt gagattctgc cgaaaactaa cgttcaccgc
     1561 ggcccgactc ttgcggaccc gctcaccggc gttggcttat tgtaggaagc aaactggatg
     1621 gaagaaccga ggtcatcacc aacgctctct ataccacttc ctggacgtaa cccgtaccgt
     1681 gagtcgctgc cagcgttacg attgtagcca atcagtaact cgtggccata gcgacgcccg
     1741 aaaacgtgag cggaagaaac caaagtgacc aatcgtttgg ctttcgacgt cgcgaggcgc
     1801 gatctgtacg cttgagctgt cgcagttcat gaacaaactc agaattcggc ctgtggctgc
     1861 ggcggttgca aagggatcgt ccgagaaccg tagcgcaagc gttctcgcac ggatcgactg
     1921 gaatcggtca agcaatgcgc cgatgagtgc ttgatccagc gagccgtctt cagatctgag
     1981 gttccaccaa aagtcaggcc tccgtcgcga ggcccactgt aaagctacac ctttcggaag
     2041 gtcttccaac acatgcaggc ggcggcccgg gaag
//
LOCUS       DATIFQ010000171         4455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2164164, whole genome shotgun sequence.
ACCESSION   DATIFQ010000171 DATIFQ010000000
VERSION     DATIFQ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164164"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VLE22_07760"
     CDS             complement(<1..800)
                     /locus_tag="VLE22_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14339.1"
                     /translation="MKSGPDRRTFLSGSAAALAAAALSSAQQREIRTAIIGVGVRGSS
                     LLRQVMEQANVKVAAICDTDPTALDAAMTAAASHNPRSFTDYRRVLDLNDVEAAYIAT
                     PCDLHTPMSIAALEAGKYVYCEKPLGVTPEQVQLAWNAARKAGTFLQIGQQLRYMPGI
                     REAIRQVHEQRVIGQPFVIKAQRHSTPPLPGKERKRPAWYLDVKRSGDLIVENAVHNL
                     DVCNWIAGSLPVSAYGHGKKYLPKPEPAGTAFMDGFSVMYVYENDVELS"
     gene            complement(797..2002)
                     /locus_tag="VLE22_07765"
     CDS             complement(797..2002)
                     /locus_tag="VLE22_07765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB14340.1"
                     /translation="MRRVALWIPTVTMLLVSLISYIDRNTLALLAPTILKDTHLTAEQ
                     YGFVISAFSVAYMIGNPVWGRWLDRFGLRTGMAVAVALWTLASTSHALAAGFLSFAIA
                     RAALGFGEGATFPGGLRTVMQTLPPSKRSRGVAVAYSGGSLGAIVTPILITPIALWWG
                     WQAAFLFTGLIGATWLAVWFLVSRRSDVKEHRRHSLSAEGAQDAPRFKDPRLWSFMLA
                     YAFGALPIALIIYGGALYLNRALGASQALIGKVLWIPPLGWEIGYFFWGWLADRMTVE
                     GKSPITPIFRLLTAATVLSLPLAFIPAVSSFGLVMFQLFLAMFVAGGILILTVAYATR
                     TYSPSHSGLIAGLGAGAWSAAVACVMPVFGRLFDLQRYDLAFLLAAACPVLGYLIWLW
                     INRDIVKPR"
     gene            complement(2154..3374)
                     /locus_tag="VLE22_07770"
     CDS             complement(2154..3374)
                     /locus_tag="VLE22_07770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HSB14341.1"
                     /translation="MGGRRIVVTGLGVISAIGRTVPEFWDSLVAGRSGIGPMETVDRS
                     QFRFQNGAEVRGYAPPDYFEEKEITLLDRFAQFALIACSEAVRDAGVEWSRETSARTA
                     IVTGSCLGGQVSQDQAFYDVYWEKRGRIQPLSIPRVMANAATSHISMKYGITGPAFTV
                     STACSSANHAIGQAFWMVRDGTADMAIAGGSDAPFAFAMLKAWEAMRVVAPDTCRPFA
                     KDRKGMILGEGAGILILEPLEAAQARGARIYAEIAGFGMTADAHHITQPSVDGPAAAM
                     RAALADAALDLTQVGYINAHGTGTPVNDPAEVTAIRKAFGDHAERLAVSSTKSMHGHA
                     LGAAGALEAVATVLALHHGIAPPTANFTEPDPECDLYVVPNQARRLDVEAALSNSFAF
                     GGLNAVLAFRRWGE"
     gene            complement(3382..3651)
                     /locus_tag="VLE22_07775"
     CDS             complement(3382..3651)
                     /locus_tag="VLE22_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HSB14342.1"
                     /translation="MDDELTKRVMAVVAATQRIPAETINPESTFAELGVDSLDGVNLL
                     FALEEEFNINIPDEDARQIRGVPDMVAGIRKLLDGAGGSAAQPRR"
     gene            3975..>4455
                     /locus_tag="VLE22_07780"
     CDS             3975..>4455
                     /locus_tag="VLE22_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14343.1"
                     /translation="MAALVLIPFLSLTPAYAGLVWDQEVITSGQGLAQDNILSFQVTG
                     SESGCVKWTGSGDAYNSSCPGEVGGDPYSWADTDTGKSRTFTLAELGTNYGITSADTL
                     GIIFNPAEPGGDSVTLNVLVLTFYSADGSTSESFMWTGGPESYCCNNTGTGGAGQVFR
                     "
BASE COUNT          856 a         1438 c         1379 g          782 t
ORIGIN      
        1 gacaactcga cgtcgttctc gtaaacgtac atcacgctga agccgtccat gaacgcagtg
       61 cccgcgggct ccggcttcgg caggtacttc ttcccgtggc cgtacgcgct cacgggcagg
      121 ctgcccgcga tccagttaca gacatcgaga ttgtgcaccg cgttctcgac gatcaggtcg
      181 cccgagcgct tcacgtccag ataccatgcc ggccgcttcc tctccttgcc cggcaacggc
      241 ggcgtgctgt gccgctgcgc tttgatgacg aacggctggc cgatcacgcg ctgctcgtgg
      301 acctggcgga tcgcttcccg gatgcccggc atgtagcgaa gctgctgccc gatctgaagg
      361 aatgtccccg cctttcgcgc ggcattccac gcaagctgca cctgctccgg cgtcacgccg
      421 agcggcttct cgcagtagac gtacttgccg gcttcgagcg ctgcaatgct catcggagtg
      481 tgcaggtcgc acggagttgc gatgtacgcc gcctccacat cgttgaggtc aagcacgcgc
      541 cggtagtccg tgaaggaacg cgggttgtgg ctggcggccg cggtcatcgc cgcatcgagc
      601 gccgtcgggt cggtgtcgca gatcgcggcg accttgacgt tcgcttgctc catcacctgg
      661 cggagtagcg aggagccgcg tacgccgacg ccgatgatcg cggtacgaat ttcacgctgc
      721 tgggcggagc taagggccgc agccgcaagt gcggcggcgg aaccggaaag aaacgttcgt
      781 ctatcaggac cggatttcat ctcggcttta ctatatcgcg gttgatccag agccagatca
      841 gatagccgag caccgggcag gccgccgcca gcaggaacgc aaggtcgtac cgctggaggt
      901 cgaacaagcg cccgaacacc ggcatcacac acgccaccgc cgccgaccag gcgcccgccc
      961 ccaggcctgc aatcagaccc gagtgcgacg gcgagtaagt gcgcgtggcg taggcgacag
     1021 tcagaatcag aatccctccc gcgacaaaca tcgccaggaa taactggaac attaccagcc
     1081 cgaatgacga caccgctgga atgaaggcca gtgggagact cagcaccgtc gccgccgtca
     1141 gcaggcggaa gatcggcgtg atcggtgact tgccctccac cgtcatccgg tccgcaagcc
     1201 agccccagaa gaagtagccg atctcccagc cgagcggcgg aatccacagc accttcccta
     1261 tcaatgcctg cgaagcgccg agcgcgcgat tcaggtacag cgcgccgccg taaatgatga
     1321 gcgcgatcgg caaggcgccg aacgcgtaag cgagcatgaa gctccacaaa cgcggatcct
     1381 tgaagcgcgg cgcgtcttgt gccccttccg ccgaaagcga gtgacgccgg tgttccttca
     1441 cgtcggaacg gcggctgacc agaaaccaca cagcgagcca cgtcgcgccg atcagtccgg
     1501 tgaacaggaa cgccgcttgc cacccccacc agagtgcgat cggagtgatc aggatgggag
     1561 tgacgatggc gccaagggag ccgccgctgt aggcgacagc gacgccccgc gaacgcttcg
     1621 acggcggaag tgtctgcatc accgtccgca ggccgccggg aaacgttgct ccctcgccga
     1681 acccgagagc ggcccgggca atcgcgaagc tgagaaagcc ggctgcgagg gcgtgcgagg
     1741 tcgatgccag cgtccacaac gcgaccgcca cggccatccc ggttcgcagc ccgaaccggt
     1801 ccagccagcg gccccacacc gggttcccga tcatatacgc gacggaaaac gcggagatga
     1861 caaatccgta ctgttccgcc gtcaggtgcg tgtctttgag gatcgtgggc gccagcagcg
     1921 cgagcgtgtt gcggtcgatg tagctgatga gcgacaccag cagcatcgtg acagtcggaa
     1981 tccagagagc gacgcgacgc acaggaatcc gcccatgata ccaaccagta gggcaggcca
     2041 tcgccttctg tggcctgccg cgcaatgtcc tgagcgtagt ttgcgacggg ggacaggctt
     2101 actccgccct gagcgcagtt tgcgagtggg cagagctgcc tgtccccctc gccttactcc
     2161 ccccacctcc ggaacgccag caccgcgttc aacccgccaa acgcgaagga gttggacaga
     2221 gccgcttcca catccaggcg cctggcctgg ttcggcacga cgtagaggtc gcactccgga
     2281 tcaggttccg tgaagttcgc cgtcggaggc gcaatcccgt gatgcaaggc cagcacggta
     2341 gccacagcct caagagcgcc tgccgcgccg agcgcgtgcc cgtgcatcga cttcgtggag
     2401 ctcaccgcga gccgctccgc gtggtcccca aacgccttcc ggatcgcggt tacttcggcg
     2461 ggatcgttga ccggcgtccc ggttccatgg gcattgatgt agcctacctg tgtcaggtcc
     2521 aacgccgcat cggccagggc cgcacgcatc gccgcggcgg gcccgtcgac agacggttgc
     2581 gtaatatggt gggcgtcggc ggtcatcccg aatccggcga tttccgcata gatccgcgct
     2641 cctcgggcct gcgccgcctc aagcggttcc agaatcagaa tcccagcgcc ctcgccgagg
     2701 atcatgcctt tgcggtcctt ggcgaaaggc cggcaagtat ccggggcgac gacacgcatg
     2761 gcttcccacg ctttcagcat ggcgaatgcg aacggcgcgt cgcttcctcc ggcgatcgcc
     2821 atgtcggcgg tgccgtctcg taccatccag aaagcctggc cgatcgcgtg gtttgccgag
     2881 gaacacgcgg tcgagaccgt gaacgccgga ccggtgattc cgtacttcat ggaaatatgg
     2941 ctggtggccg cgttggccat gactctcggt atgctgagcg gctgaatgcg tcctcttttc
     3001 tcccagtaaa cgtcgtagaa cgcctggtct tgggagactt ggccgcccag acaggagcct
     3061 gtcacgatcg ccgtccgggc gctggtttcc ctgctccatt cgacgcccgc gtccctgacc
     3121 gcctcgctgc aggcgatgag agcaaattga gcaaagcgat cgaggagggt gatttctttc
     3181 tcttcgaaat agtccggcgg agcgtagccg cgaacctcgg ccccgttttg aaaccggaac
     3241 tggctccggt caacggtctc catcggaccg atgccgctcc ggccggcaac aagcgagtcc
     3301 cagaactccg ggaccgtcct cccgatcgcg gagatgactc cgaggccggt gacgacgatt
     3361 cggcgccctc ccatcgcggg gctagcgcct cggctgagcg gcggaaccac cggctccgtc
     3421 aagaagcttg cggatacccg ccaccatatc cggcacgcct cgaatctggc gcgcgtcctc
     3481 gtcgggaata ttgatgttga actcctcttc cagggcgaag agcaggttga ctccgtcgag
     3541 cgagtcgacc cccagctcgg caaacgtgct ttccgggtta atggtctccg cgggaatccg
     3601 ctgcgtggcg gcgaccaccg ccatgacccg cttcgtcagt tcgtcatcca taaggcgttc
     3661 cggtcaatcg gtgtcctggc ggcacggcgg ccgcgcccgg aatcccgact cgaaccagcg
     3721 tacaacaaat attcggggaa ccgaaacgga aacatttcca cacgattctg tgcaatcgcg
     3781 ccgcacatcg agggcaaatg tactagtacc tctccgtggt actgaacgtc ctccatctgc
     3841 ttggatagga ggcgtttaat ccttgaatcg gatggagatt cgctgtaacc tcgaatttgg
     3901 cactttgatc ctgtagaaag aaaactgcga gggtgctgga acgaatgcgg gggagaaaaa
     3961 cgaagaaatt attgttggcg gcgttggttc tgattccatt cttgtcgtta actcccgcat
     4021 acgcgggtct cgtttgggac caggaggtca tcacctccgg ccaaggtctc gcacaggaca
     4081 acattctgtc gttccaggtg accggctccg agtccggttg cgtgaagtgg actgggagcg
     4141 gagatgcgta taattcgagc tgtccgggag aggtaggcgg tgatccgtac tcctgggctg
     4201 acacggacac cggcaagtcg cgcacattta ccctggccga gcttgggacg aactacggga
     4261 tcacgtcggc ggataccctt ggcatcatat tcaatccggc ggagccgggc ggcgacagcg
     4321 tcactctcaa cgtcctcgtg ctgaccttct acagcgccga cgggagcact tctgaaagct
     4381 tcatgtggac cggagggccg gagtcgtact gctgcaacaa caccggaacc ggcggtgccg
     4441 ggcaggtatt ccggt
//
LOCUS       DATIFQ010000172         8968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_250027, whole genome shotgun sequence.
ACCESSION   DATIFQ010000172 DATIFQ010000000
VERSION     DATIFQ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250027"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VLE22_07785"
     CDS             complement(<1..270)
                     /locus_tag="VLE22_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14344.1"
                     /translation="MNYRFVRRKAVNVVMFSVTGISAAITVSVLFFILGYLLWHGATA
                     LNWDFFTQLPKPVGEAGGGMANAIVGTGKILLLATLIGVPIGFIAG"
     gene            complement(267..1250)
                     /gene="pstC"
                     /locus_tag="VLE22_07790"
     CDS             complement(267..1250)
                     /gene="pstC"
                     /locus_tag="VLE22_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HSB14345.1"
                     /translation="MASSAVQIDNRGTRGHAWALVRRLRDGDELAHILTLTVASLILL
                     ITVLLVFELYRSSALSREKFGWGFFYSTAWDPVAGEFGALPFIYGTVVTSIVALVIAV
                     PLGVAAAIFLSELAPPKISDALTFLVELLAAVPSVIYGLLAIFVLVPLLRETLQPTLK
                     STFGFLPFFQGPVLGVGLLAAGLILAIMILPFIVSISREALLAVPREQREAALAIGAT
                     RWEATWQVVVPFARLGIAGSVFLALARALGETMAVTMVIGNNPKIGASLFDAGYSIAA
                     VIANEFTEATDDLHLSALIELGLVLFLLTIVINGLARVLILATTPGGSSQP"
     gene            complement(1255..2262)
                     /gene="pstS"
                     /locus_tag="VLE22_07795"
     CDS             complement(1255..2262)
                     /gene="pstS"
                     /locus_tag="VLE22_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HSB14346.1"
                     /translation="MRRAIPALLLLAVTIPALSQVLVNGAGATFPYPIYAKWFDEFHK
                     KNPQAQINYQSIGSGGGIRQLLAGTVDFGASDSPMTDEQLSKSPTRVLHFPTVLGAVV
                     ATYNVPGVTQELNFTPEVLSGIYLGTVTKWNDPVLAKANPRAKLPSAGIIPVRRSDGS
                     GTTFVWTDFLAKASPAWKSKVGSGNAVSWPAGLGAKGNEGVSGLVKQTPNSIGYVELV
                     YAIQNKMPYGRIQNASGTFVRPDLKSVTAAAASAAANMPDDFRVSITYAAGKDAYPVS
                     SFTWLLLPSSIQDGGKRKILTDFLRWMLHEGQAMAEPLGYAPLPKAVIANELKTIGKI
                     R"
     gene            2470..3015
                     /locus_tag="VLE22_07800"
     CDS             2470..3015
                     /locus_tag="VLE22_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14347.1"
                     /translation="MRLRAPALMAFVAFGFLRAEGLPVCLVDRAGLDPITLSAFEKEL
                     QSLLPEGSAILHRAPCDVERPTRSVRLTIRSEPPRQLPKALGVAYLSATGQVLPILDV
                     YLSRLSSYLNWPRTPYVVGRALARVAAHEFGHFLVQDAGHHQDGLMRSQFPYWLLADP
                     DPTPFRMAANARPPFRLLSSR"
     gene            3165..3923
                     /locus_tag="VLE22_07805"
     CDS             3165..3923
                     /locus_tag="VLE22_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14348.1"
                     /translation="MKAILRTSLLCCLATASLLAQGSLSVINGARFELRYPVSPGSYA
                     QAYGVYPGVQTEFAQTVPLPPQLGGVQVLVAGTAAPLVAVTSEAVSFQVPRNTPIGRA
                     EVSVRKDGTVLATGSVDVVPVSPGVFFVLTDPLSQGGVLNDISLYAVKETPAQRGKAI
                     QIFGTGQGPVDIDVPDGAAPPSGTLAHARAATKVYISVDEAEVTFSGLSPQFPGLWQI
                     NATVPNKTYVSGQVPLVVTIDGVPSNQTSFWVAE"
     gene            3965..6742
                     /locus_tag="VLE22_07810"
     CDS             3965..6742
                     /locus_tag="VLE22_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB14349.1"
                     /translation="MNKTKTLSCLVALLAALAITPSYGQAGEEEGSMIVGAVFDAANG
                     QPVRGAKVFVKDQPSIATVTDTDGQYRLSVPPGQYTLAIQAANYLDTAIEAVDAKPGE
                     PVQASTVMAAVGSVTTVEVVEKAGAATATAEAMLAERKLAPVISDTLSNEEIKKSTAS
                     DAAAVLEKVTGVSVVQGGYVYVRGLGERYSATMLNNALLPTTEPERRVVPLDLFPASL
                     IDNIKVLKTYTPDLPGEFAGGLVQMQTVDFPSARMFRVSVSTGFNSRTSFDRFGTYPG
                     GGSDFFGFDDGSRSIPSSIPADRLFQGSFSDEEFQAFGRSFPDNWESRPIDSMRPSQT
                     YTVAGGDTFGRVGLVGALTFSNKPQRIQEMQRYLTNSGNGNPVILTEYPDYTSNMESA
                     RLGGVLNAAIRITPANKLVFRNLLTRDSDKEARVFSGYNGGTDTYIQAERLRWIERGL
                     YSSSVEGEHAVQRLGNSLFHWQFTFSSSTRDEPDLREVVRGRMDDGSYAFLALNESGL
                     RFYNGLKDRIYEPQAEWGKPFFAGRVSGMLKVGFRGTFRTRDFWARRFRFIPNRVITL
                     DLLAPSNELFGPSDIRPDGFQVLEYTRGTDTYDADMDVYGGFAMVDLSLGPRWRLIGG
                     TRIEDASVNVHTIDPRVPGAVPTTAALNNRDALPSINLIYALTPRQNLRFGYGRTLSR
                     PDFRELSPFEFLNVLGGFSVIGNPNLKRASIDNFDARWEWFRGGDELFAVSYFYKDFT
                     DPIEVTIRPTTGDLRESFLNAKAARNQGVELEFRKNLGSLSRTLAQFNVHSNFTVVDS
                     NVKIPDGQDLLLTSRQRPLVGQSRYIANVITEWFRPKWRSNARFYANAVSRRITDVGS
                     VGLPDIYQERSMFLDFVYQFDIVENGKWSIRFSAENLADNNYRWTQAGILQRSFQLGR
                     TYNVGTSFSIF"
     gene            6794..8599
                     /locus_tag="VLE22_07815"
     CDS             6794..8599
                     /locus_tag="VLE22_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14350.1"
                     /translation="MTARFLVGAGVLSLFCLGVQAAEKGGGVNFASVGYGEAEREVSV
                     LAQFDTPAAVWLWTDKYVYQPGQSLTLRWTVKPNNDLYPYTIVAYRQNNQTGAKTYLP
                     NGTAEATDIFGNTVQQGFRITRLPEANKQVLIGAGGMFPAGLGTVPNEPGMHTLVVQI
                     RDYTGTRIVKSAYWKIGVVDSFEDLQGDVTADKTLVRTKAYSLKGLVFVKNNATLTIE
                     PGTFILGQPGSQPPSALFVSQNGKIIADGTRSRPIIMTSSQPIGERKPGDWGGLVLLG
                     KAPVNWATGFGYIEGVTESDDTKYGGSDPNHNCGTLRYVRVEFAGSALSPNNEVNGIT
                     WGACGKGTITDHVEAKYGFDDNFEWFGGNSDAKYLVSAYPRDDYIDGQIGWTGRLQHV
                     VAVANKEYQGNRGIEMDNNEKDFGAQPLSKPQMYNFTFVGAGDAFTQGVDEGTGVAAL
                     WLRRGTGGVYNNMILYNWVSGGFEARDDATLARIDSKDLTANGILMWDNGKLSGKANT
                     VEGQSTSTAAPFLNGQRGEAKNVEVTDPMLRRPLEGSDPDFRPRLGSPVFRANWIQPP
                     DDGFFDQWAAWIGAFGDQDWTEEWSSFHQEDDLKP"
     gene            complement(8707..>8968)
                     /locus_tag="VLE22_07820"
     CDS             complement(8707..>8968)
                     /locus_tag="VLE22_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB14351.1"
                     /translation="TATEFRLLEFLMGRPEVVFNREQLLDAVWGHDRAVTDRTVDVYI
                     LRLRQKIESDPANPEMIRSVRGFGYCFAAVTSATEETATTSK"
BASE COUNT         1898 a         2637 c         2675 g         1758 t
ORIGIN      
        1 tcccgcgatg aagccgatcg gtacgccgat cagcgttgcc agcagaagaa tcttaccggt
       61 gcctacgatc gcattggcca tgcccccgcc ggcctcaccg accggtttcg gcaactgcgt
      121 aaagaagtcc cagttcagtg ccgtcgcccc atgccaaaga aggtagccga ggatgaaaaa
      181 cagcacggag acggtaatcg cagcggagat gccggtaacg ctgaacatca cgacattcac
      241 cgccttgcgc cttacgaagc ggtagttcat ggctgtgaag atcccccggg ggtggtggca
      301 aggatcagga cccgcgccag tccgttgatt acgatggtga gcagaaacag caccagtcca
      361 agttcaatca gcgcgctcag gtgcagatcg tctgtggcct cagtgaattc attcgcaatt
      421 acagccgcaa tcgaataccc ggcgtcaaac aacgaagcgc cgattttcgg gttgttaccg
      481 atgaccattg tgaccgccat cgtctcgccc agcgcccgag cgagcgcgag aaacacggac
      541 ccggcgatac cgagccgagc gaacggcaca accacctgcc aggttgcctc ccaacgcgtg
      601 gcgccgatcg cgagggccgc ttctctctgc tctcgcggaa cagcgaggag cgcttccctg
      661 gatatcgaga cgatgaaagg aagaatcatg atcgccagga tgagcccagc ggccagcagg
      721 ccgacgccga gaaccggccc ctgaaagaat ggcaggaacc cgaacgtact cttcagggtc
      781 ggctgtaatg tctcgcgcaa caacggcacc aggacgaaaa tcgcgagcag tccgtaaatc
      841 acgcttggca ccgcggccag cagctcgacc agaaaggtga gtgcgtcgga aatcttcggc
      901 ggcgccagtt cggacaggaa gatcgccgcc gcgaccccga gcggcaccgc gatgaccagg
      961 gcgacaatcg aggtcaccac agtaccatag atgaacggaa gcgccccgaa ctcgcccgcc
     1021 acggggtccc atgccgttga atagaagaat ccccatccga atttctcacg ggacagcgcc
     1081 gagcttcggt ataactcgaa aaccagcaga accgtgatca gaaggatgag ggacgcgacc
     1141 gttaacgtca gaatgtgcgc gagttcgtcg ccatcccgga ggcgccgcac cagtgcccac
     1201 gcatgcccac gtgtgccccg gttgtcgatc tggaccgcgg aacttgccat ggagttaccg
     1261 gatttttccg atcgtcttca gttcgttcgc gataacagcc ttcggaagcg gggcgtatcc
     1321 cagaggctct gccatcgctt gtccttcgtg aagcatccag cgaagaaaat ccgtcaggat
     1381 cttgcgtttc ccgccgtcct ggatgctgga gggaagcagc agccaggtga agctcgatac
     1441 cggataagcg tcctttccgg ctgcgtacgt gatggagaca cggaagtcgt cgggcatgtt
     1501 cgcggcggcg gaggctgccg ccgcggtcac gctcttgaga tcgggcctga cgaaggtgcc
     1561 ggacgcattc tggatacggc catacggcat cttgttctgg atggcgtaca ccagctccac
     1621 atatccgatc gaattcggcg tctgctttac cagtccggag acaccctcgt tacccttggc
     1681 tccgaggccg gcgggccaac tcaccgcgtt ccccgaaccc acctttgatt tccacgccgg
     1741 gctggccttt gcgagaaagt ccgtccagac gaatgtcgtg ccgctgccgt cagaacgccg
     1801 caccggaata atgcctgcgg agggcagttt cgcgcgcggg ttggccttcg cgaggaccgg
     1861 atcattccat ttggtaaccg ttccaaggta gattccggac aggacttccg gagtgaagtt
     1921 cagctcctgt gtcactcccg gcacgttgta ggtggccact accgccccga gcacggtcgg
     1981 aaagtggagg acgcgcgtag gcgacttcga gagctgctcg tcggtcatcg ggctgtcgga
     2041 cgccccgaag tcgaccgtcc ccgccagcaa ctgccggata ccaccgccgg aaccgatgga
     2101 ctggtagttg atctgtgcct ggggattctt cttgtggaac tcgtcaaacc acttggcata
     2161 gatggggtac gggaaggtag ctccggcccc gttgaccagg acctggctaa gagcggggat
     2221 cgtcacagcc agcagcaaca gagcaggaat tgcgcgtctc acaaattgtc tccttgtaac
     2281 attctcgaaa ccaaacgtta caattcaatt acatttcaat gaattgtcgg tttgtgggat
     2341 ggccggctag gcccaacacg ggggcaggcc gtgatcgaag aaaaatcaaa atgccgtgac
     2401 aatattatta caaaacggct acaagcccgg caaactgttg tatgatcgga tctgaaggcg
     2461 attgttacaa tgaggttacg agctccggcg ctcatggcgt ttgtcgcttt cgggttcctg
     2521 cgggcggaag ggttgcccgt ctgcctcgtc gaccgagcag ggcttgaccc gatcacgctg
     2581 agtgcctttg agaaagaact ccagtcgctg ctgcccgaag gctccgcaat cctgcatcga
     2641 gcgccgtgtg atgtggaaag acccacacgt tcggttcgtt tgaccatccg ttcggaacct
     2701 ccacggcaac tcccgaaggc ccttggcgtg gcctatctgt ccgccacagg acaggttcta
     2761 ccgattctcg acgtctattt gtcgcggctc tcctcgtact tgaactggcc tcgcacaccc
     2821 tacgttgtcg gacgggcgct ggcccgagtg gccgcccacg agtttgggca cttccttgtt
     2881 caagatgcag gccatcatca agacggactg atgcgcagcc agttccctta ctggctgctt
     2941 gcagatcctg accccacacc tttccgaatg gcggcgaatg cgcggccgcc cttccgtctt
     3001 ctctcatccc gctaggccaa aaggctagcg atcgaattca caaaaccgca acacgaaatt
     3061 tcttgcttgt taacagccgg ctgttaactt aaggctctgg tgacggccga gagtgttgtg
     3121 cgaccgcgat agatctctat gactacgcaa acaaaggaaa gccgatgaaa gcaattctaa
     3181 ggacctctct gctttgctgc ctcgctacgg cgagtctgtt ggcacaaggt tcgctctccg
     3241 tgatcaatgg cgcaaggttt gaattgcgtt acccggtttc tccgggttca tacgcgcagg
     3301 cgtatggtgt gtacccgggt gtacagaccg agttcgccca aacggtgccc ctgcccccgc
     3361 agctcggagg tgtgcaggta ttggtagcag gcaccgcagc acctcttgtc gcggtgactt
     3421 cagaagcggt cagtttccag gttccgagaa acactccaat tggacgggcg gaagtcagtg
     3481 tgagaaaaga tggcacagtt ttggcgacgg gatcggtgga cgtcgtgccg gtttcacccg
     3541 gagtcttctt cgtcctgaca gatccgctct ctcaaggtgg tgtgctcaac gacatcagcc
     3601 tgtatgcagt gaaggagacc ccggcacaac gcggcaaggc gatccaaatc tttggaaccg
     3661 gccagggccc ggtcgacatt gacgttccgg acggcgccgc tccgccctcc ggcacgctcg
     3721 cacatgcgcg cgcagccact aaagtctaca tctccgtcga cgaagcggag gttacgttca
     3781 gcggcctgtc gccgcagttt ccgggacttt ggcagatcaa cgccactgtt ccaaacaaaa
     3841 cctacgtttc aggtcaggtg ccattggttg tcacgattga tggcgtgcct agcaaccaaa
     3901 ccagtttctg ggtagccgag tagcaaggta gcactttact tggagaaaga cagcggtagt
     3961 gaaaatgaac aaaacaaaga ccttgagttg tttggtcgcg ctgctggcag cactggcgat
     4021 caccccgtca tatggccagg ccggagaaga agagggaagc atgattgtcg gcgccgtgtt
     4081 tgacgcggca aacggccagc cggtccgcgg cgccaaggtc ttcgtcaagg atcagccgtc
     4141 gatcgcgacg gtgacggaca cggacggcca gtacaggctt tcggtaccgc ccggtcaata
     4201 cacactggcc atccaggcgg cgaattacct ggacacggcc atcgaagcgg tggatgccaa
     4261 gcctggcgaa ccggtgcagg cgagcacggt aatggcggca gtaggaagcg ttaccactgt
     4321 ggaggttgtg gagaaggccg gagcggcgac tgctacggcc gaggcgatgc tggctgagcg
     4381 gaagctggct cctgtgatca gcgacacgct cagcaacgaa gagatcaaga agtcgacggc
     4441 ctctgacgcc gctgccgtcc tcgagaaagt cactggcgta tccgtcgtac agggcggcta
     4501 cgtctatgtg cggggtctcg gggagcgcta cagcgccaca atgctgaaca atgccctgtt
     4561 gccgacgacc gaaccggaac ggcgcgtggt gccgctcgat cttttccctg cttcgctgat
     4621 cgacaacatc aaggtgctca agacttacac tcccgacctg ccaggcgagt ttgcaggcgg
     4681 cctggtgcag atgcagacag tcgatttccc atcggcgcgc atgtttcggg tcagtgtcag
     4741 caccgggttc aacagccgca catctttcga tcggtttggc acctatccgg gcggcggcag
     4801 cgacttcttt ggattcgatg acggcagccg cagcattccc agttcgatcc cggcagatcg
     4861 cctgttccag gggagttttt cggacgagga gttccaggcc ttcggccgtt catttccgga
     4921 caactgggag tcaaggccga tcgactcgat gcgcccctct cagacctaca cggtcgccgg
     4981 cggcgacacg ttcggccgcg tcgggcttgt gggcgcgctc acgttcagca acaagccaca
     5041 gcggattcag gaaatgcaga ggtatctgac caattccggc aacgggaacc cggtgattct
     5101 gacggaatac ccggattaca cctccaacat ggagtccgct cgacttggcg gagtgctgaa
     5161 cgccgccatc cggattacgc ccgcgaacaa gttggtgttt cgaaacctcc tcactcggga
     5221 cagcgacaag gaagcccgcg tcttcagcgg ttacaatggc ggcaccgaca cttatattca
     5281 ggcggagcgg cttcgatgga tcgaacgcgg cttgtattcg agcagcgtgg aaggcgagca
     5341 tgcggtccag cggctgggca acagcctttt ccactggcag ttcacgtttt cgtcgtccac
     5401 ccgtgacgag cccgatcttc gtgaagtcgt tcgcggccgg atggatgacg ggagctacgc
     5461 atttctcgcc ctgaacgagt ccggattgcg tttctataac ggactcaagg accgcatcta
     5521 cgaaccgcag gcagaatggg gaaagccgtt ttttgcgggg cgtgtcagcg gcatgttgaa
     5581 agtcggtttt cgcggcacat tccgcacccg tgacttctgg gcgcggcgat tccggtttat
     5641 tccgaaccgc gtcatcactc tcgatctgtt ggccccttcg aatgagctct tcggcccgag
     5701 cgatattcgc ccggacggct ttcaggtgct cgagtacact cgcggcactg acacctacga
     5761 cgccgacatg gatgtctatg gcgggtttgc gatggtcgat ctctcgctcg gcccccgctg
     5821 gcggctcatc ggcggcactc gcattgagga cgcctccgtc aatgtgcaca cgatcgaccc
     5881 aagagtgcct ggagcggtgc cgacgacggc tgcgctgaac aaccgcgacg cgctgccaag
     5941 catcaacctg atttacgctc tgacccccag gcagaacctc cgtttcggct acgggcggac
     6001 gctctcccgt cccgacttcc gcgaactgtc cccgttcgag ttcttgaacg tgctgggcgg
     6061 ctttagtgtg atcggtaatc cgaacctcaa acgggccagc atcgacaact tcgatgcacg
     6121 ttgggagtgg ttccgcgggg gcgatgagtt gttcgcggtc agctattttt acaaggactt
     6181 cacggacccg attgaggtga cgatccgtcc gaccaccggc gaccttcgag agagcttcct
     6241 taatgcgaag gccgcgagaa accaaggagt ggaactcgag tttcggaaga atcttggctc
     6301 acttagccgg acgctcgccc agttcaacgt ccactccaac ttcaccgttg tggactcgaa
     6361 cgtcaaaatc ccggatggac aagacttgct cctgacctcc cggcagcgac cgctggtggg
     6421 gcaatcgcgg tacatcgcca acgtgatcac ggagtggttc cgtccgaagt ggcggagcaa
     6481 cgcgcgcttc tatgcgaacg cggtttcgcg ccggattacc gacgtcggct cggtggggct
     6541 tccggacatc tatcaggagc gcagcatgtt cctggacttc gtctaccagt tcgacatcgt
     6601 cgaaaatggc aagtggtcga tccggtttag cgcggagaac ctcgcagaca acaactaccg
     6661 ctggacgcag gcgggaatcc ttcagcgcag tttccagctt ggccgcacat acaacgtcgg
     6721 aacgagcttt tcgatttttt gacagaacgc acactcttga atgaggagag gaactgaaac
     6781 catgcaggct agaatgacag cacgttttct ggtcggggca ggagtactga gcctgttttg
     6841 cctcggcgtg caggcagcgg aaaaaggcgg aggagtgaac ttcgcttctg tcggttacgg
     6901 tgaggccgag cgagaagtgt ccgtcctcgc ccaattcgat actccggcag ctgtctggct
     6961 ctggacggac aagtacgtct accagccagg tcaatcgctg acccttcgct ggacagtaaa
     7021 gcccaacaac gatctgtacc cgtacacgat cgtggcgtac aggcagaata accagacggg
     7081 cgctaaaacg tatcttccaa acggaacggc ggaggcgacg gacatctttg ggaacaccgt
     7141 ccagcaaggc tttcggatca cacggttgcc ggaagcaaac aagcaggtgc tgatcggcgc
     7201 cggggggatg tttcctgcgg gtctcgggac ggtgccgaac gagcctggaa tgcacaccct
     7261 ggttgtgcag atccgggact acacaggcac ccgcattgtc aagtcggcgt actggaagat
     7321 cggtgtcgtc gacagctttg aggaccttca gggtgatgtc acagccgaca agacgctggt
     7381 gagaacaaag gcgtacagtc tgaagggtct cgtcttcgtg aagaacaatg ccactttgac
     7441 gatcgagccg ggcacgttca ttctcggcca gccgggctct cagccgccgt cggcgctgtt
     7501 cgtatcacag aatggcaaga ttatcgcgga tggcacccgc agccgcccaa tcattatgac
     7561 cagctcccag ccgatcggtg agcggaagcc aggcgactgg ggtgggctgg tccttctggg
     7621 caaggctccc gtgaactggg ccaccggctt cggctacatc gagggggtca cggaatccga
     7681 cgacactaag tatggtgggt ccgatcccaa ccacaattgc ggaaccttac gttacgtccg
     7741 cgtagagttc gccggatcgg ctctcagccc gaataacgag gtcaacggca tcacgtgggg
     7801 cgcctgcggc aagggcacca taaccgacca tgtcgaagct aagtacggct tcgacgacaa
     7861 cttcgagtgg tttggcggga actccgacgc gaagtatctg gtttcagcat atccgcgcga
     7921 cgactatatc gatgggcaga tcggctggac cggccggctc cagcatgttg ttgcggtggc
     7981 gaacaaggag taccagggga atcgcggcat cgaaatggac aataacgaga aggacttcgg
     8041 cgcccaacct ctcagcaagc cgcagatgta taacttcacc ttcgtcggcg ccggcgatgc
     8101 gttcacccag ggcgtggatg aaggaactgg cgtcgcggct ctctggctcc gccgcggcac
     8161 cggcggggtc tacaacaaca tgatcctcta caactgggtg tccggcggct tcgaagcgcg
     8221 cgacgacgcc actttggcca ggatcgacag caaggacctc acggccaacg gcattctcat
     8281 gtgggataac ggcaagcttt ccggcaaagc caacaccgtc gaggggcagt caacatccac
     8341 cgcggcgccc ttcctgaacg ggcaacgcgg agaagcgaag aacgtggaag tgacagatcc
     8401 gatgctccgc aggcctcttg aaggtagtga tcccgatttc cggccgcgtc tgggttcgcc
     8461 ggttttccgg gcgaactgga tccagccccc ggacgacggt ttcttcgacc agtgggcggc
     8521 gtggattggc gctttcggtg atcaggattg gaccgaggaa tggtccagtt tccaccagga
     8581 agacgatctt aagccgtaaa ttacagcgac tgttgtcttt ctccaagccc cggggtctcg
     8641 accccggggt atttttggga tggcaagagg tcctccgagg gtaacgtacc gttatgcaat
     8701 gacgaattac ttcgacgttg tcgcggtttc ttcggtcgca gaggttaccg cggcaaagca
     8761 atatccgaac cctcgaaccg aacgaatcat ttcgggattt gccggatcag actcgatctt
     8821 ctgcctgaga cggaggatgt agacgtcaac ggtccgatcc gtgaccgctc tatcgtgccc
     8881 ccacaccgcg tcgagcaact gctcgcggtt gaaaacgact tcggggcggc ccatcaaaaa
     8941 ctcgagcagg cggaactcag tcgcggtc
//
LOCUS       DATIFQ010000173         9242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1207761, whole genome shotgun sequence.
ACCESSION   DATIFQ010000173 DATIFQ010000000
VERSION     DATIFQ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207761"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /locus_tag="VLE22_07825"
     CDS             <1..1940
                     /locus_tag="VLE22_07825"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB14352.1"
                     /translation="VTISYVEILKYEAGTYEFMFPMVVGPRYIPGQPTGRQGGGWAPD
                     TDHVPDASRITPPVAKPGTRAGHDISVEVSLDAGVPIDALRATTHEVDVERPGTNRAV
                     VRLRDKAVIPNKDFILRYDVAGRKIEDAVLTHRAGRGGFFTLILQPPERVTAEDVTPK
                     ELVFVLDTSGSMSGFPIDKAKETMKLAIDGLYPQDTFNLITFAGDTRILFPEPVPATP
                     ENVRKAQEFLASRSGGGGTEMMKAIRAALDPSDSQSHIRIVCFMTDGYVGNDMEIIAE
                     VQKHPNARVFSFGIGSSVNRFLLDKMAEQGRGEVEYVALNDDGSAAARRFHERVRSPL
                     LTDVTVDWSGLPVTDVYPKRIPDLFSAKPVVLTGRFSAPARGFIHLRGMISGRPVTRD
                     IRVDLPAAEPRHDVLATLWARTRVDDLMGQDYAGIQQGTARTDVREAITQLGLDYRLM
                     TQFTSFVAVEEMTVTTGGEPRRVEVPVELPEGVSYEGVFGKDEAPQMMRAKSFSGVGG
                     VVGGVMSSAKPMAAPARLAQAIEVDRRDAPAITPAERKRRELLSKLHPALAAIVERIE
                     KRSPNPAPEERRLVKNGKTDVQIWLANTTPETIAALKRLGFELVLAPKTAKMVIGRLP
                     VEKLAELAKLNAVRYVAPAAG"
     gene            complement(1947..4064)
                     /locus_tag="VLE22_07830"
     CDS             complement(1947..4064)
                     /locus_tag="VLE22_07830"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HSB14353.1"
                     /translation="MVRPAATAARLADRFFELSLLGLVSSGFLAVAGSGYLDAPTAIL
                     TAAGLLLRALLVLGIVRFEISPRTVALLTLAYLGFYALDYLAISRDFLGATVHLVCFL
                     AVVKILTARTNRDFVYVKIIAFLELLAASILSTNVNFFVFLALFLLFAVATFASSEIR
                     RTTRRPGAVARGGLRRFHLRLASLSVVIALGVLSLTAGLFFLLPRTAHAAFQRLASER
                     YQLPGFSNEVRLGQIGEILQQNTPVMHVRVEGKVPAYMLRWRGAALSSFDGRRWYNPV
                     TTGNLLRVEEGRLIVAGDDQRRREGSRVLYEVQVEPTVSDALFFAGVPEVLWINSPMV
                     LRSATDTYRLGYPSSTGLRYHASSYVDPELRAGDRGVWPPSTAPSKDYLQLPPEDARV
                     RELASRVTGGSASDEQRARAIAAYLRRSYAYTTELPVRAVSDPIAHFLFERKKGHCEY
                     FASSMAVMLRTIGIPSRVVTGFQTGVFNPISGWYVVRASDAHSWVEAWMAGQGWTTFD
                     PTPPDLSRRSPALLAKLALYMDAAEMFWQDWVVGYDRERQLTLALKMGDSGRGFGTRW
                     RDQVRLAAFALWTRTAVFLGRFGFFLLCLAGFVILLRVAGPELWRRLWTLVRVQKVRR
                     GEAQASDATILYERMLALLKRRGFERPAWLTPGEFARQLPPSATADVVRRLTAAYNEL
                     RFGGNLEAAPVITSLLDQLERSA"
     gene            complement(4111..4515)
                     /locus_tag="VLE22_07835"
     CDS             complement(4111..4515)
                     /locus_tag="VLE22_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14354.1"
                     /translation="MSRRDCLMLAVAAAGCGRSERPPDELLPISPGEGWTRSDVRTAP
                     PAEAPDTVRQLGPRRVIHARYSGPGDVELTIYEMGSAVSAFELNQKWRVAENAVHFHH
                     GPYLVVAESGGAGKPRLFAFARLIEQHLKSLQ"
     gene            4545..6431
                     /locus_tag="VLE22_07840"
     CDS             4545..6431
                     /locus_tag="VLE22_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14355.1"
                     /translation="MLLSLFHDNPPSASYAERFEKERVLERLRSLDAKVRRDGPATLD
                     PAKDLLARIGTESDETGRVTKYSYGVFNLSWQAQRHPEWAQLVTAEVREIQQRIRERH
                     NRPLRFLVWAGMGGSAEDKSMYNAAGLLRRGVRCYVLDSTDPSKLKHILEDMRRRSGL
                     TTADVLRSSLVVGMAMGMTSYEPVINLEKLAALYQKHGIDSRPNFLYMTLPGSLLDRF
                     ARKHGYRRVELQLDNGNTTAGRHSSPLTRGSLYPLALCRADLRGWIEGTFLSDEEIRS
                     AWRLSAFIHAQGTADRDKLTLLLPKPWAGACIWTKQNFEESLGKCEKLGLKIVIEDRP
                     KLANYRSPKDPLRQRSFFAVQMKGARDEETKKVSLLRRSGYPVAVLALPEGTPLSRYM
                     QFIHYTVFGVAYLREMNFVTQPGVELYKAITNRIYSEARNAGGIDKSRAWQSFMKSDR
                     RVEWRGAITLHHSHAGTAGDTAGMEAPQLYAHLVKQLHGEGKVDYAELTFFGDMRYSP
                     RGRRMRTRLDRAAESLFRSRLKLPVDIHEGPAMNHSYHEMIIGHGKCFSTVLIADRQE
                     TLPAAEYTADYHRAQFLATQLALAERRRPVVAITVKDLEEDTLQRLDDFFHQAAAHLR
                     TARI"
     gene            6452..6586
                     /locus_tag="VLE22_07845"
     CDS             6452..6586
                     /locus_tag="VLE22_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14356.1"
                     /translation="MGKFKLAGRKKKSQKPGGAIPCIALIVIGIVLLSLLFYAILKSG
                     "
     gene            6590..7309
                     /gene="rph"
                     /locus_tag="VLE22_07850"
     CDS             6590..7309
                     /gene="rph"
                     /locus_tag="VLE22_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HSB14357.1"
                     /translation="MRSDNRLPGQMRPVHIVTDYLKTAEGSALIEVGSTRVLCAASVE
                     QTVPQFLRGGGKGWVTAEYSMLPRATVTRTPREISRGKPSGRTQEIQRLIGRSLRSVV
                     DLTALGEQTIILDCDVLQADGGTRTAAITGAFVALGLAVKRLVEYGALKTMPIRDYVA
                     AVSAGIVGGEALLDLCYEEDSRAEVDMNMVMTGRGGFVELQATAEQSPFADGQLGELL
                     ALGRNAIVQLVETQRGILKLW"
     gene            7306..7905
                     /gene="rdgB"
                     /locus_tag="VLE22_07855"
     CDS             7306..7905
                     /gene="rdgB"
                     /locus_tag="VLE22_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HSB14358.1"
                     /translation="MTLYCGTGNPGKLREFRLAIEHFGEGRLSVEPVPGLSGVPPCEE
                     TGATFEENAVLKAVYYSQLAPGLLFADDSGLEVRALGGAPGVSSARFAGPGATDEANN
                     RLLLEKLRGVEDRAAQFVCMIALADAGKLVATFRGIIEGHILHEPRGANGFGYDPLFY
                     HPPFACTFGEATGEQKLAVSHRGRALAVMVKYLLSRKTA"
     gene            complement(7921..8760)
                     /locus_tag="VLE22_07860"
     CDS             complement(7921..8760)
                     /locus_tag="VLE22_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSB14359.1"
                     /translation="MDFNEFLKNSLQHNAVASVVLLVVVVLLRFVLRRAVLRNVAQPE
                     LRLKWLVNIRNGLLIGFVLGLGSIWAEALRPFAVSVVALAVAFVIATKELIQCVSGSV
                     VRATGKFYSIGDRIEIGGYRGDVIDQSLLSTTILEVGPGRAFHMYTGRTVTFPNSLLL
                     TAPVLNESSTDRYIVHAFTIPLRADEDWKRAEAILLEAAGAECEPFLEEAKSQMEALE
                     RTYGFGSPSAEPRVAVQISDPGSIRLMLRIPCPVGRQGRLEQAILRRFLGKFHGESEP
                     GKD"
     gene            complement(8777..>9242)
                     /locus_tag="VLE22_07865"
     CDS             complement(8777..>9242)
                     /locus_tag="VLE22_07865"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSB14360.1"
                     /translation="VDPDGFEIRRAIVQHRGSAVMMPVDKRDRILLVRQYRLPAGNSL
                     WELPAGRVDEGERVLAAARRELAEETGYRARRWKKLLSVFVSPGYVAEKMTIFVATDL
                     VEGDASPMEDERITCRWFAPSEVDAMIRSGRIADAKTIVGFLAWQRYRRAKK"
BASE COUNT         1804 a         2956 c         2952 g         1530 t
ORIGIN      
        1 aggtgaccat cagctacgtc gagatcctga agtacgaggc gggcacgtat gagttcatgt
       61 ttccgatggt cgtcggcccg cgttacatcc ccgggcagcc caccggcagg caggggggcg
      121 gctgggctcc cgataccgac cacgtgccgg acgcctcgcg gattaccccg ccggtggcaa
      181 agccgggtac tcgcgcgggt cacgacatct ccgtcgaagt ctccttggac gccggcgtgc
      241 cgattgacgc gctccgcgcc accacgcacg aggtcgatgt cgagcgaccg ggcacgaacc
      301 gggccgtcgt tcgcttgcgc gacaaagccg tcatcccgaa caaagacttc attcttcgct
      361 acgacgtcgc cggtcgaaag atcgaggacg ccgtactcac tcatcgcgcc ggccggggcg
      421 ggttctttac gctgatcctc caaccgccgg agcgcgttac ggccgaggac gtgacgccga
      481 aggaactggt gttcgtgctg gacacgtccg gatccatgtc cggattcccg atcgacaaag
      541 ccaaggagac gatgaagctc gcaatcgacg gcctctaccc ccaggacact ttcaacctca
      601 ttaccttcgc cggtgatacc cgtatcctgt tcccggaacc ggttccggcg acgccggaga
      661 acgtccgcaa ggcgcaggag tttcttgctt cgcgctcggg cggcggaggc accgaaatga
      721 tgaaagcgat ccgtgcggcg ctcgatccgt cggattcgca gtcccatatc cggatcgtct
      781 gcttcatgac cgacggctac gtcggcaacg acatggagat catcgccgag gtccaaaagc
      841 accccaacgc acgcgtcttc tcctttggaa tcggcagctc ggtgaaccgc ttcctgcttg
      901 acaagatggc cgagcagggc cgcggagagg tcgagtacgt cgctctgaac gacgacggct
      961 cggcggcggc gcggcgcttc catgagcgag tccgcagccc gctgctaacc gacgtgacag
     1021 tggactggag cggtctgccc gtgaccgacg tttacccgaa gcgaattccg gatctgttca
     1081 gcgcgaagcc tgtcgtgctg accggacgct tctcggcgcc ggcccggggt ttcatccacc
     1141 tgcgcgggat gatttcgggc aggcccgtga caagggacat ccgcgtggac cttcccgctg
     1201 ccgaaccacg ccacgacgtg ctcgcgacgc tgtgggctcg gacgcgcgtg gatgacctga
     1261 tggggcagga ctacgcgggc atccagcaag gcacagcgcg caccgacgtc cgtgaggcga
     1321 ttacgcagct cgggctcgac tatcgcctga tgacgcaatt cacctcgttt gtcgccgtcg
     1381 aagagatgac cgtcaccacc ggcggagagc cgcgaagagt cgaagtgccg gtggagttgc
     1441 cggagggcgt cagctacgaa ggggtgttcg gcaaggacga ggcgccacag atgatgcgcg
     1501 cgaagtcgtt ctccggtgtt ggcggcgtag tgggcggcgt catgtccagt gctaaaccga
     1561 tggcggcgcc cgcacgtttg gcgcaggcaa tcgaggtcga ccgccgggac gctcctgcca
     1621 taacgccggc cgagcgcaag aggcgggagt tgctgtcgaa gctgcacccc gcgttggcgg
     1681 cgatcgtcga gcggattgag aagcgcagcc cgaatccggc gccggaggag cgccggcttg
     1741 tcaaaaacgg caaaaccgac gtgcagatct ggctcgcgaa caccacacca gagacgattg
     1801 cggcgctcaa gaggttgggc ttcgaactag tgctcgctcc gaagaccgcc aagatggtga
     1861 tcgggcggct gcccgtcgag aaactggccg agctagcgaa gctgaacgcc gtgcgctatg
     1921 ttgcgccggc ggccgggtga ggcggctcag gcggagcgct cgagctggtc gagcaacgac
     1981 gtgatgacgg gggcggcctc caggttgccc ccgaatcgca gttcgttgta cgcagcggtt
     2041 agcctgcgca ccacgtcagc cgtcgcagac ggcggcaact ggcgggcgaa ctcgcccggc
     2101 gtgagccacg ctggcctctc gaacccgcgt cgcttgagaa gggcaagcat gcgttcgtag
     2161 aggatcgtgg cgtccgaggc ctgggcctcg ccccggcgca ctttctggac gcgcaccagc
     2221 gtccacaggc ggcgccagag ctccggtccg gccacacgaa gaagaatcac aaagcccgcc
     2281 aggcacaaaa ggaagaaccc aaaccgtccg agaaacaccg cagtccgcgt ccacaaggcg
     2341 aacgcagcca gccgcacctg gtcgcgccag cgcgtgccga agccgcggcc cgaatccccc
     2401 atcttgaggg ccagagtcaa ctggcgctcg cgatcgtacc ccaccaccca gtcctgccag
     2461 aacatctcgg ccgcgtccat gtacagcgcc agctttgcca gcagcgccgg ggaccgccgc
     2521 gacaggtcag gcggggtcgg atcgaacgta gtccacccct gccccgccat ccaggcttcg
     2581 acccaactgt gcgcgtccga ggcccgcacc acgtaccagc cgctgatcgg attgaacaca
     2641 ccggtctgaa agcctgtaac cacccgggaa ggaatgccga tcgtgcgcag catgactgcc
     2701 atcgaagagg cgaagtattc gcagtgccct tttttccgct cgaacagaaa gtgggcgatc
     2761 ggatcgctca ctgctcgcac gggcagctcc gtggtgtagg cgtaacttct gcggagatac
     2821 gccgcgatgg cgcgggcgcg ctgttcgtcg gaggcggaac cgcccgtgac acggctggca
     2881 agttcgcgga ccctcgcgtc ttcgggcgga agctgcaggt agtctttcga tggggccgtg
     2941 gagggcggcc agacgccgcg gtcaccggcg cgcagctcgg ggtcgacata gctgctcgcg
     3001 tggtagcgaa ggccggtgga gcttgggtaa ccgagacggt aggtgtccgt cgcgctgcgc
     3061 aacaccatcg gagagttgat ccaaagcacc tcggggactc cggcgaagaa tagcgcatcg
     3121 gagaccgtgg gttcgacctg cacctcataa agaacacggc tgccctcgcg cctgcgctgg
     3181 tcgtccccgg cgacaatgag tcggccttcc tcgacccgga gcaggttgcc cgtggtgacc
     3241 gggttatacc agcggcggcc gtcgaagctg ctcaacgccg cgccgcgcca tcgcagcatg
     3301 tacgccggga ccttgccctc gacgcgaacg tgcatcaccg gcgtgttctg ctgaagaatc
     3361 tcgccgatct gcccgagacg gacttcattt gagaaacccg gcagttggta acgctcggaa
     3421 gcgaggcgct ggaaggctgc gtgcgcggtc cgcggcagca ggaagaaaag ccccgccgtc
     3481 agcgacagga cgccgagcgc gatcacgaca ctgagcgagg cgaggcgcag atggaaacgc
     3541 cggaggccgc cgcgcgccac ggcgcccggc cggcgggtcg tgcggcggat ttccgagctg
     3601 gcgaacgtcg ccaccgcgaa taacagaaaa agggccaaaa atacgaagaa attgacgttc
     3661 gtcgaaagga tcgaggccgc cagcagctcg agaaaggcga tgatcttcac gtagacgaag
     3721 tcgcggttgg ttcgcgccgt caggatcttt acgacggcga gaaagcacac cagatgcact
     3781 gtggcgccga gaaagtctcg ggaaatcgcc aggtagtcca gcgcatagaa accgaggtag
     3841 gccagcgtga ggagggccac cgtgcgcggg gagatctcga atcgcacgat gccgagaacc
     3901 agcaaggcgc gcaggaggag tcccgcggcg gtcagaatcg cggtcggagc gtccagatat
     3961 ccggagccgg cgacggcgag aaagccgctc gaaaccaaac ccagcaggga cagctcgaaa
     4021 aaacggtccg ccaggcgggc ggcggttgca gccggacgca ccattgcatc ttcattgtat
     4081 caacgcacgc cttaacggcc ctggcgtgcg tcactgcagc gatttcaagt gctgctctat
     4141 cagcctcgcg aacgcgaaca gccgcggctt tccggcgccg cctgactcgg ccaccaccaa
     4201 atacggcccg tggtgaaaat ggaccgcgtt ttcggcgacc cgccacttct ggttcagctc
     4261 gaaggcgctg actgccgagc ccatctcgta gatggtgagc tcgacgtctc cgggaccgct
     4321 gtaacgggcg tggatgacgc gccgggggcc aagttgccgg acggtatccg gcgcttccgc
     4381 gggaggcgcg gtgcgcacgt cactccgtgt ccaaccctct ccgggcgaga tcggaagcag
     4441 ttcgtccggc ggacgctcgg accgcccgca acctgcggcg gcaacggcga gcatgagaca
     4501 atcgcggcgg ctcatccgca tgccttatat gctaaaacga gtaggtgctt ctctcgctgt
     4561 tccacgacaa tccgccctcc gcaagttacg ctgagcgatt cgaaaaagag cgggtgctgg
     4621 aacggctccg gtcgctggat gcgaaggtgc ggcgcgacgg gcccgctacc ctggatcccg
     4681 ccaaggacct gctggcgagg attgggaccg agtccgatga gaccggccgc gtcacgaaat
     4741 actcgtacgg tgtcttcaat ctgagctggc aggcgcaacg gcatcccgag tgggcccaac
     4801 tggtgaccgc cgaggtgcgg gagatccagc agcgaatccg tgagcgccac aatagacccc
     4861 tgcgattcct ggtctgggcg ggcatgggag gttcggccga ggacaagtcc atgtacaatg
     4921 cggccggatt gctccggcgg ggcgttcgct gttacgtact cgactcaacc gatccgtcga
     4981 agctcaagca cattctcgag gacatgcgga ggcggtccgg attgaccact gccgatgtcc
     5041 tgcgaagctc gctggttgtc ggcatggcga tgggcatgac gtcgtacgag ccggtgatca
     5101 acctcgagaa acttgcggcg ttgtaccaga agcacggcat cgacagccgt ccgaactttc
     5161 tctacatgac gctgcccggt tcgctgctcg accggttcgc gcgcaagcac ggctatcggc
     5221 gggtcgaact gcagctcgac aatgggaata ccacggccgg acgtcacagc agcccgctga
     5281 ctcgcgggag cctatacccg ctggcgctgt gccgggcgga tctgcgcggg tggatcgagg
     5341 gcacgttcct ctcggacgag gagatccggt ccgcgtggcg gctctcggcg ttcatccatg
     5401 cccaggggac ggccgaccgg gacaagctga cactgctatt gcccaagcca tgggccggcg
     5461 cttgcatctg gacaaagcag aacttcgagg aaagcctcgg caagtgcgaa aaactcggtt
     5521 tgaaaatcgt catcgaggat cggccgaaac tcgccaacta ccgctctccc aaagaccccc
     5581 ttcggcaaag gtcgtttttc gccgtccaga tgaagggcgc gcgtgacgag gaaacaaaga
     5641 aggtctccct tctgcggcgc tcagggtatc cggtggccgt gcttgcgctg ccggaaggta
     5701 ccccactctc gcggtacatg cagttcattc attacacggt cttcggggtg gcatacctgc
     5761 gcgagatgaa cttcgtcacg cagcccggcg tcgaactcta caaggcaatc acaaaccgca
     5821 tttactcgga agcgcggaac gccggcggca tcgacaagag ccgggcgtgg cagagcttca
     5881 tgaagtccga ccggcgggtc gaatggcgcg gagcgattac actccatcac agccacgccg
     5941 ggaccgctgg agatacggcc ggcatggagg cgccgcagct ctacgcgcac ctcgtgaagc
     6001 aattgcacgg cgagggaaaa gtggactacg cggagctgac gttcttcggc gatatgcgtt
     6061 actcgccccg agggcggcgg atgcgaaccc ggctggaccg cgccgcggag tccctcttcc
     6121 gttcgcggct aaagctcccg gtcgatatcc atgagggacc ggcgatgaat cactcgtatc
     6181 atgagatgat catcggacat gggaaatgct tctccacggt gctaatcgcc gacagacagg
     6241 agacgttgcc cgccgcggaa tacacggcgg actaccaccg cgcgcagttc cttgccacac
     6301 aactcgcgct cgcggagagg cgccgtcccg tggtggcgat cacggtaaag gacctggaag
     6361 aggacacgct ccaacggctg gatgacttct ttcaccaagc tgcggcgcac ttgaggaccg
     6421 cccggatttg agacgatagg atcgaccgcg catgggtaaa ttcaagctgg cgggaagaaa
     6481 gaagaagagc cagaagccgg gcggagcaat accatgtatc gctcttatcg tcatcggcat
     6541 cgtcctgctg tccctgctgt tctacgcgat cctgaaatcg ggttaagcaa tgcgatcgga
     6601 taaccgtctg ccgggccaga tgcgcccggt gcatatcgtc accgactacc tgaagaccgc
     6661 cgagggctcc gctctgatcg aggtcggcag cacacgggtg ttgtgtgccg cctctgtgga
     6721 gcagacggtg ccgcaattcc tccggggcgg cgggaaagga tgggtaacgg cggagtattc
     6781 gatgcttccg cgcgccaccg tcacgcgaac gccgcgcgag atttccaggg ggaagccctc
     6841 cggccggacg caggagatcc agcggctgat cggacggtcg ctgcggtccg tagtcgacct
     6901 gaccgcgctc ggcgagcaga cgatcatcct ggactgcgat gttctccagg ccgacggtgg
     6961 cacccggacg gcggcgatca ccggagcctt cgtcgccctc ggcctcgccg tcaaacgcct
     7021 cgtcgaatac ggtgcgctga agacgatgcc gatccgggac tacgtcgcgg cggtcagcgc
     7081 cggtattgtc ggcggtgagg cactgctcga tctttgttac gaggaagact cgcgagccga
     7141 agtcgacatg aacatggtca tgaccggacg cggggggttt gtcgaattac aggcaaccgc
     7201 cgagcagtcg ccgtttgctg acggacaact cggtgagttg ctcgccctgg gccgtaatgc
     7261 gatcgtgcaa ctggtcgaga cccagagggg cattctcaag ctgtggtgac actctactgc
     7321 ggaaccggta atccgggaaa actccgcgag ttccgcctcg ccatcgaaca cttcggcgaa
     7381 ggacgtctct ccgtcgagcc ggttccagga ctttcgggcg ttccgccctg cgaggaaacc
     7441 ggcgccacct tcgaggaaaa cgccgttcta aaagccgtct actacagcca gctcgctccc
     7501 ggcctgctct tcgctgacga ttccggactg gaagtgcgcg cgctcggcgg cgcgcccggc
     7561 gtctcctcgg cgcgtttcgc cggccccggg gccaccgatg aggccaacaa ccggctgctg
     7621 ctcgaaaagc tgcgcggcgt cgaggaccgg gccgcccaat tcgtctgcat gatcgcgctc
     7681 gctgatgcag gcaagctcgt ggcgaccttt cgaggaatca tcgagggcca catcctccac
     7741 gaaccgcgcg gcgcgaacgg cttcgggtat gacccgctgt tctaccatcc gccgttcgcc
     7801 tgcaccttcg gcgaggccac cggtgaacag aaactggcgg tcagccaccg cggccgcgcg
     7861 ctcgcggtga tggtgaaata cctgctgagc cggaagactg cgtgacgccg ggtcgaacaa
     7921 tcagtccttg ccgggctcgc tctctccgtg aaacttcccc agaaagcgcc gtaatattgc
     7981 ctgctcgagg cgcccctgcc ggcccacggg acatggaatg cgcagcatga ggcggatcga
     8041 tcccgggtcc gagatctgta cggccacgcg cggctcggcc gatggactgc cgaagccgta
     8101 cgtacgctcg agcgcctcca tttggctctt cgcctcttcc aggaaaggct cgcactcggc
     8161 gccggcggct tccagcagga ttgcttccgc tctcttccag tcttcgtccg cccgcaacgg
     8221 gatcgtaaac gcgtgcacga tatagcggtc ggtcgaagat tcgttcagga ccggagcggt
     8281 cagtagcagg ctgttcggga aggtcaccgt gcgacccgta tacatatgaa acgctcggcc
     8341 cggtcccact tcaaggatcg tcgtgctgag caggctttgg tcaatcacgt cgccgcggta
     8401 gccgccgatc tcgattcggt ctccgatcga gtaaaacttg cccgtcgcgc gcaccaccga
     8461 gccgctgaca cattgaatca gctcctttgt ggcgatcacg aacgcgacgg ccagcgcaac
     8521 caccgaaacg gcgaacggcc gcagcgcttc ggcccagatc gaccccagcc ccagcacgaa
     8581 gccgatcagc agcccattgc ggatgttgac cagccatttc agcctcagtt ccggctgtgc
     8641 aacattccgc agaacggccc gccgcaggac aaaccgaagc agcacgacaa ccacgagcag
     8701 aacgaccgac gcgacagcat tgtgctgcag ggagtttttg agaaattcat tgaagtccat
     8761 gacgggctgg ttcagtttac ttcttcgccc tccggtaccg ttgccaggcg aggaatccga
     8821 cgatcgtctt tgcgtcagcg atccggcctg agcggatcat cgcatcaacc tcgctcggcg
     8881 cgaaccaacg gcacgtgatc cgctcatctt ccatcggcga cgcatcgccc tcgacgagat
     8941 cggtagccac aaagatagtc atcttctcgg cgacataacc gggactgaca aaaaccgaaa
     9001 gtagtttctt ccagcggcgc gcgcgatagc cggtctcctc ggcgagctct cttctggctg
     9061 cagcgagtac cctctcgccc tcgtcaaccc tgccggccgg caattcccaa agcgagttcc
     9121 cggctggcaa acggtattgg cgcacgagca gaatccggtc cctcttatcg accggcatca
     9181 taaccgccga cccgcggtgc tgtacgatcg cccggcggat ttcgaatccg tccggatcga
     9241 cc
//
LOCUS       DATIFQ010000174         3476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2549075, whole genome shotgun sequence.
ACCESSION   DATIFQ010000174 DATIFQ010000000
VERSION     DATIFQ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2549075"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VLE22_07870"
     CDS             <1..460
                     /locus_tag="VLE22_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14361.1"
                     /translation="NDEAVEDDGEETTKEVSAMDAFLACPASQFWGYYKYVNEDSPFS
                     TQQGIKGAEFERVLVVLDDDEGTHVQFSYDKYLGIKPLSDRDEANRREGKETVVERTR
                     RLFYVCCTRAMKDLIVVLFTADVEAARRQITQLGLFPAHTIHLEDELEAN"
     gene            1270..2268
                     /locus_tag="VLE22_07875"
     CDS             1270..2268
                     /locus_tag="VLE22_07875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HSB14362.1"
                     /translation="MAHVRATKQDGSEPHLESWVPEELRILPDPQTAIPAIAKNLRLM
                     SLIDNAVRTIPTRHQLFFRDSRLLDDLAPGSIHLALTSPPYWTLKEYRHSAGQLGDVA
                     DYDTFLSELDQVWQHCFHALVPGGRLICVVGDVCLSRRKNDGRHTVVPLHASIQEHCR
                     RIGFDNLAPIIWHKIANAAYEVESGSAGFLGKPYEPNAVIKNDIEFILMQRKPGGYRK
                     PSVPVRVLSVISTENYQRWFQQIWTGLTGASTREHPAPFPIELAERLIRMFSFVGDTV
                     LDPFMGSGTTNVAAARCGRNSLGCEVDEQYFDLAERRIRDETTGLFTSVHIEAHRQ"
     gene            2278..3018
                     /locus_tag="VLE22_07880"
     CDS             2278..3018
                     /locus_tag="VLE22_07880"
                     /inference="COORDINATES: protein motif:HMM:NF016442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HSB14363.1"
                     /translation="MPETNDHLLKEVQRAVRHFWRTRERQAKAQGGKTGERDRGARSA
                     VTGGAHLDGFADLIRNLVVDAGVSNTAVHRRSRVELPGYYRAEKKWDLVVVVDGRLLA
                     TVEFKAQVGPSFGNNYNNRTEEALGNAADYWAAFREGAFRGSPRPWLGYMMLLEETSG
                     SKRPVSVAEPHFPVFAEFRNASYARRYEILLTKMVRERLYDAASLILSPSDAGRNGTF
                     AEPCSELRFANFAESLVARAIAYSRTQR"
BASE COUNT          614 a         1104 c         1064 g          694 t
ORIGIN      
        1 taacgacgag gctgttgaag atgacggtga agagactacc aaggaagttt ccgccatgga
       61 tgcctttctc gcgtgcccgg cgtcgcagtt ctggggatac tacaagtacg tgaacgagga
      121 ctctccgttc tcgacgcagc aaggcatcaa aggcgcagag tttgagcgcg tgcttgttgt
      181 cctagacgat gatgagggta cccacgttca attctcctat gacaaatact tgggaatcaa
      241 accgctctca gacagggatg aggcaaacag gcgcgagggc aaagaaacag ttgtagagag
      301 gactaggcgt ctcttttacg tttgctgtac acgcgccatg aaggatctga ttgtcgttct
      361 ctttacggcg gatgtcgaag cggcgcgacg ccagatcacg cagctgggtc tttttccggc
      421 gcatacgatc catctggaag acgagcttga ggcaaactga tctttcgcac atcagggggg
      481 acgtcagggg ggacgttggt tctaaagcgc attgtgtttc tggggtccgg agcagttctg
      541 ccgcgttccg gaggtctgca tccgacggaa cccccagatt cctccctcct gccccctgct
      601 cacaactggg tggtgtacag cgttcggctg agcgttcgat cccgccgcgg gcgggactca
      661 cgccgaagtc tcgcggagac gcctcgtgcg ctccacccac cgccccttcg atcccgctgc
      721 tcctcagggg tccgtcccgc gggtccgcgg gattcggatt gtcgcgactc cggtcagggt
      781 ggccatctcc aatgaccggc tggtcagcgt ggaggcggag gagtggcggt cctgttcgat
      841 cctgcccctg gtgggactaa cggcaggctt cgtggccgcc ttccggccgg gcgacatccc
      901 agcccctcga ggggctcggg gtgggttccg ctaccggaca cctcgtgatt tcgcccttgg
      961 cgagccggtg gcccctcggg ttgtcaccgg ccctccggcg ggtcgggtga ctcccggctc
     1021 ccgcgggcgt ccctggcctg tagcctcgca agcctcttcc ctcagccccg cggttcggat
     1081 acaatccccg taacttgtag cccaccggtt cagttcaacc cgtcttaccc gctggactcg
     1141 ctcccgcggt cgcagctcgg ttctacgtgg agcttgccgg ataagagcct attcgttgcg
     1201 cggcacgcag gaaacaggtg acggcagagg tctttcgtgg tacggaatga gacgcggagg
     1261 caacgagcca tggcccatgt tcgcgccacg aagcaggacg gttctgagcc tcacctcgag
     1321 tcttgggtgc ccgaggaact gcgaatcctt cctgacccgc agactgccat tccggccatc
     1381 gcgaagaacc ttcgtttgat gagcctcata gacaatgctg ttcggacaat tccaacgaga
     1441 caccagctct ttttccgcga ctcccggttg ctcgacgacc ttgcgccggg ttctattcac
     1501 ctcgcgctta cttctccgcc atattggacc ctcaaggaat accgccattc cgccggccaa
     1561 cttggcgatg tggcggatta cgacacgttt ctctccgagc tcgaccaggt atggcaacac
     1621 tgctttcacg cgctcgtccc tggaggccgc ctgatctgcg tggttgggga tgtctgcctc
     1681 tctcgccgca agaacgacgg ccgccacacg gtggtgcccc tgcatgcgtc catccaggag
     1741 cattgccgtc ggatcggctt tgacaacctg gcccctatca tctggcacaa gatcgcgaac
     1801 gcggcctacg aagtggagag tggatcggcg ggtttcctgg ggaagcccta cgaaccgaac
     1861 gctgttatca agaacgacat cgagttcatt ctgatgcaac ggaagccggg cggctaccgg
     1921 aagccttctg tccccgtgcg agtcctgagc gtcatctcga ccgagaacta ccagcgctgg
     1981 tttcaacaaa tctggacggg cttgaccgga gcctccacgc gtgagcaccc cgcccccttc
     2041 cctatcgagt tggcggaacg gctgatccgg atgttcagct tcgtcggtga tacggtcctc
     2101 gacccgttta tggggagcgg caccacgaat gtggcagccg cgcgttgtgg tcgcaacagc
     2161 cttgggtgtg aagttgacga gcagtacttc gacctagccg agcggcgcat cagggacgaa
     2221 accacagggc ttttcacttc cgtccacatc gaggcccaca gacaatagcg gtgcgcattg
     2281 cctgagacta acgatcatct tctcaaggaa gtccaacgag ccgtccgcca cttctggcgg
     2341 acccgcgagc gccaggcaaa agcccagggt ggcaagactg gagagcgaga ccgcggcgcc
     2401 cgcagtgctg tcacaggcgg agcgcacctc gacggattcg ccgatctgat ccggaacttg
     2461 gttgtagacg ctggtgtcag caacaccgct gtgcaccggc gcagtcgggt tgagctgccc
     2521 ggctactacc gtgcggagaa gaagtgggac cttgtcgttg tggttgacgg tcgcctgctg
     2581 gcaacggttg agttcaaggc acaggtgggg ccgtccttcg gcaacaacta caacaaccga
     2641 actgaagagg cccttgggaa cgccgcggac tactgggctg ctttccgcga gggcgcgttc
     2701 cgaggctcgc cgagaccgtg gctcggatac atgatgctcc tggaggaaac ctccggctcc
     2761 aagcgtccag tgtccgtcgc ggagccccac ttccccgtgt tcgccgagtt ccgcaacgcc
     2821 tcatacgcaa ggcggtacga gatcctgctc acgaagatgg tgcgcgagcg gctctacgat
     2881 gcggcgagcc tcatcctgtc tccaagcgat gccgggcgga atggaacgtt cgcggaacca
     2941 tgctcggaat tgaggttcgc caatttcgcg gagagcctcg tggcacgcgc cattgcctat
     3001 tccaggaccc agcggtgacc gcgcgctcca gcagttccct tgagctcacg acgacgcctc
     3061 gggcgctcca cccacctcct cttcgatccc gccgccccgc aggagtccgt ccgcctcagg
     3121 cgtattcgga cttgcgggac tccggacagg gtggccgtct ccaatgaccg gccggtcagc
     3181 gcggatgcgg gggaggtctg cgtcgcttcg aatggactta aaggaggtcg gtggggggac
     3241 tcctcctgag aaagccaatg acctgccgcg tatcctttgg gtcgccgcgc tggtacgcgc
     3301 ggagggactt cgcatgtgtc ccctcgtgct cccgccggcc ctccggtggc tcgaactgcc
     3361 tccgcaggtc cttgggccag ccccctctcc cgcccaccgt tccccgaagt gggtgtgggt
     3421 tccgacttga cactgctttt ccacccatgg tatgcgtccc gtgctggcgg cctccg
//
LOCUS       DATIFQ010000175         2646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1782521, whole genome shotgun sequence.
ACCESSION   DATIFQ010000175 DATIFQ010000000
VERSION     DATIFQ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782521"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VLE22_07885"
     CDS             complement(<1..1349)
                     /locus_tag="VLE22_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14364.1"
                     /translation="MGEPARVPPPSPRTPEERLDSWKEIAAYLNRDVTTVQRWEKREG
                     MPVHRHQHDRMGSVYAFRADLDAWARSRNLRTAQDEGSNAPLPTPAALPTRSATLTSP
                     SRWKFAVLLAAATLLAIGGSLWLQQSEYFWRNPIAEARFQKVTDFDGVEQAAAVSRDG
                     QFVAFLSDRDGPMDVWVTQAGSGQFHNLTRGSIPELVNPSVRTLGFSPDGSLVTFWAR
                     KENGTKAGDISIWAVPTLGGQPRPYLEGAAEFDWSSDGSRLTYHTPGPGDPLFVSRGI
                     GRSQEPAIFTAAAGLHSHFPLWSPDGKFIYFVQGSLPDKLDIWRIPPTGGAPQRITSH
                     VGRVSHPVLLERRTLLYLATDPDGSGPWLYNMDVERRIPHRLSSGLDRYTSLAASADG
                     RRLVVTLASPKRTLWRLRIADSATQVPAAAPISLTTGTGFSPRFGPNYLLYVSATGT"
     gene            1574..2083
                     /locus_tag="VLE22_07890"
     CDS             1574..2083
                     /locus_tag="VLE22_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB14365.1"
                     /translation="MRQRVMWVLLFSAVTVVTVLYFARNVWATPANGFAATTLALGRF
                     GEIDVFNQLPQDGNVWLSLQKTKAASDLYVQSNVWQPGGSTGWHTHPGHSLIIVTAGA
                     VTAYEGNDRDCKPSVYTQGMGFVDPGGDHVHILRNEGIVEARTIAVQLIPAGAVRRFD
                     AQSPGYCPF"
     gene            2166..2363
                     /locus_tag="VLE22_07895"
     CDS             2166..2363
                     /locus_tag="VLE22_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14366.1"
                     /translation="MANMLQSCRSYEPNFADPEFSLSWQPTRAEMFPAGDTGYTVGRF
                     ELHGRDAKSNKVVRHGTYLTV"
BASE COUNT          496 a          816 c          850 g          484 t
ORIGIN      
        1 ctcgtgccgg ttgcagaaac atacaagaga tagtttgggc cgaatcgcgg cgagaaaccc
       61 gtgcccgtcg tcagggagat gggagcggcc gccggtactt gcgtagctga atccgcgatc
      121 cgaaggcgcc aaagtgttct cttcgggctg gcgagcgtga ccaccagacg gcggccatca
      181 gcgctggcgg ccagcgaggt gtaccggtca aggcccgaag acaggcggtg cggaatgcgg
      241 cgctcgacgt ccatgttata gagccacggc cccgaaccat ccggatcggt ggcgaggtac
      301 agcagcgttc gccgctccag caggacggga tgactcaccc gcccgacgtg ggacgtaatc
      361 cgttgcggag ctccgccggt tggcgggatg cgccagatgt ccagtttgtc cggaagggag
      421 ccctggacga agtagataaa cttcccgtcc ggcgaccata agggaaagtg agagtgaagc
      481 ccggcagcgg cagtgaagat ggccggctcc tgcgatcggc cgatgccgcg cgagacgaac
      541 aaggggtccc cggggccggg ggtatggtac gtgagccggg agccgtcgct cgaccagtcg
      601 aactcggctg cgccttcaag gtatggcctt ggctgtccgc ccaaagttgg cactgcccag
      661 atactgatat cgccggcttt cgtgccgttt tccttgcgag cccaaaacgt gaccagcgag
      721 ccatcgggcg agaacccaag agtgcggacc gatgggttga ccagttccgg gatgcttccg
      781 cgtgtcaagt tgtggaattg tcccgaaccg gcctgcgtca cccagacgtc catcggtccg
      841 tcgcggtccg acagaaacgc tacgaactgg ccgtcgcgtg ataccgcagc agcctgctcc
      901 acgccgtcga agtccgtgac cttctgaaaa cgcgcctccg caatcggatt tcgccaaaag
      961 tattccgact gctgcaacca gaggctcccg ccaatggcca ggagggtcgc cgctgccagc
     1021 agcacggcga acttccatcg gcttggagaa gtcagggtcg ccgagcgagt cggcagcgca
     1081 gccggagtgg gcaagggagc gttgctgccc tcatcctgag ctgtgcgaag attgcgactg
     1141 cgcgcccagg cgtccaagtc tgcccgaaag gcgtagaccg agcccatgcg gtcgtgctga
     1201 tgccggtgaa cgggcatccc ctcccgcttt tcccagcgct ggacggtggt cacgtcgcgg
     1261 ttgaggtagg ccgcaatttc cttccacgaa tccagccgct cttcgggcgt tctcggggat
     1321 ggcggtggga cacgggctgg ctcgcccatt gcgccctcct gatttcctct ttcggcagtc
     1381 cccactgatc ccaaattgta cccccgcagt ccacccccgc caaggcaaag caccgcattt
     1441 gacggcaagt cgcggaaacg gctgcagttc agcacattgc tgtttgactg ccctcgcaac
     1501 atgcccgaac gtgtcggcca cgtccgcact ccgcggcaac aacaaggatg gcaagaggaa
     1561 aaggagtctc gatatgcgac aaagagtgat gtgggtgttg ttgttcagtg ccgtgaccgt
     1621 ggtcacggtg ctctacttcg cacgaaacgt ctgggcgacc cctgccaacg ggttcgcggc
     1681 cacaacgctc gccctcgggc ggttcggcga aatcgatgtc tttaaccagc ttccgcagga
     1741 tggcaacgtc tggttgtctt tgcagaagac gaaggcggca tcagacctgt acgtgcagag
     1801 caatgtctgg cagccgggag gaagcaccgg ctggcacacg caccccggcc acagcctgat
     1861 catcgtcacc gcgggagcgg tgacggccta tgaagggaac gatcgcgatt gcaagcccag
     1921 tgtatacacg cagggcatgg ggttcgtcga tcctggcggt gaccacgtgc acatcctccg
     1981 gaacgaaggc atcgtagagg cacgcacgat agctgtccag ctcattccgg caggtgcggt
     2041 tcgtcggttt gacgctcaaa gcccggggta ttgcccgttc tgacgaggac gggctgattc
     2101 gacgctgctc gcaagggatc cctggaggtg gatcggaccc gcgtccaggg atcgctgtgt
     2161 cctcagtggc caacatgctg caatcgtgcc gttcttacga gcccaacttt gccgaccctg
     2221 aattttcgct ctcctggcag ccgactcgcg ccgagatgtt tcccgccggc gacaccggtt
     2281 acacggtcgg ccgttttgaa cttcatggcc gggacgccaa gagcaacaag gtggttcgcc
     2341 acggtactta cctgaccgtc tagaggatgc agcccgacgg cagctggaag gtgatcgccg
     2401 atggcggcgc ccccgacgcg cagtgacgtg tgccgacaca cgcgagggcg cgtgtgccac
     2461 acgctgattg cacattcccg tcgccggaag cctttcccaa tcctgcaaca tcgctttctt
     2521 gttcgtgttt gcattgccgg atctgcccgc cgcccgccct acaatttgcc caaggaagac
     2581 cctccgatgg gcttgagagt ggcggttgtg gcagtggcgt tggctgggct ggcgtggggc
     2641 ggtggc
//
LOCUS       DATIFQ010000176         2766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1976152, whole genome shotgun sequence.
ACCESSION   DATIFQ010000176 DATIFQ010000000
VERSION     DATIFQ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976152"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VLE22_07900"
     CDS             <1..732
                     /locus_tag="VLE22_07900"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB14367.1"
                     /translation="RGWRILQPFQVGPAGEFRDVVVYLEGIDKGKPFADGAVPQIEAK
                     DCLFVPFTTVVRDDQSVTVVNMDPVMHDIQAYETSHLGPRVLFNVPLPMNPAHPRNLK
                     DRSDAGLYHKHMAGAPMKQLVNLSKNRRIFVMQCGFHAYMESWGLAVTNPYFAKTDEQ
                     GRFTLTDVPPGTYKLVVWHPYVRSAIEQSVTIGPKGTVEANVAVPAPTGRLYANEVMD
                     HAYVRFGVTEETQKEIEPMVHKQAH"
     gene            841..>2766
                     /gene="hyfB"
                     /locus_tag="VLE22_07905"
     CDS             841..>2766
                     /gene="hyfB"
                     /locus_tag="VLE22_07905"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HSB14368.1"
                     /translation="MLILLWVLLAGYAVGIILPLILRRPQPQSLAASICAIVATGSGI
                     ALGLLGLRVSEPVTFSLGSTIPLLTFAIRLDALAAFFVLTISLVGLAASIYAIGYLRH
                     FHGRVSVAALGALFNAFLCSMTLVVIADNGFFFLIVWELMSLTSYFLVVTEHEKADVR
                     YAGFFYLIMTHVGTAFIIVAFLLFFQQGASFSFDAFRHPEQPLAENLRTFAFLAALIG
                     FGTKAGVVPLHVWLPYAHPAAPSHISALMSGVMIKTAIYGLLRVTFDFLDGQFPWWWG
                     FAVLTIGAVSALLGVMYALMEHDLKSLLAYHSVENIGIILLGIGAGMIFHAYGLNELA
                     ALGLVAGLYHTINHAMFKALLFLGAGSLLYATHTRNMEEYGGLLRRMPWTGFCFLIGA
                     VSISALPPTNGFVSEWLVFQSLFLSFQVPSLLVKLMLPLAAAMLALTGVLALACFAKA
                     FGISFLALPRSSHARHAEEVPWSMRIGMGLLSVICILLGLAPMIVVPMLDRITSPMAG
                     ASIANKMLALDGWALAPVNVEFSSLSAPVLALALVGTAAVALALVALAGGFRAKRYFK
                     TWGCGLNLTPRMEYTATGFVQPIKRVFSTIYQPTVKLETEFLEQSKYFAKHRRFEFHI
                     EPVFETYLYRPVVAFVSM"
BASE COUNT          463 a          867 c          821 g          615 t
ORIGIN      
        1 cgcggctggc gcatcctgca accgttccag gtggggccgg ccggcgagtt tcgcgacgtg
       61 gtggtctatc tggaggggat cgacaagggc aagcccttcg cggacggggc cgtgccccag
      121 atcgaagcga aagactgcct cttcgttccg ttcacgaccg tcgtgcggga cgatcaatcg
      181 gtgacggtgg tcaacatgga ccccgtcatg cacgacatcc aggcctatga aacctcgcat
      241 ttggggccgc gcgtgctgtt caacgtgccg ctgccgatga atccggcgca cccccgcaac
      301 ctcaaggatc gcagcgatgc cgggctgtac cacaagcaca tggccggcgc gccgatgaag
      361 caactggtga acctgagcaa gaaccgccgg atcttcgtca tgcagtgcgg gttccatgcg
      421 tacatggaga gctggggtct ggccgtcacc aatccgtact ttgccaagac ggacgagcag
      481 gggcggttca ccctgaccga tgtgccgccg ggcacgtaca agctggtcgt gtggcatccc
      541 tatgtccgga gcgcgatcga gcagagcgtc accatcggcc cgaaggggac tgtggaggcc
      601 aatgtcgcgg tcccggcgcc gacggggcgg ctctatgcca acgaggtgat ggatcatgcg
      661 tacgtccgct ttggcgtgac cgaggagacc cagaaagaaa tcgagccgat ggtccacaag
      721 caggctcact gaacccgacg tgaggcgggt gcgccgcgcc cagcaccatg acgcgaggac
      781 ccatattgtg cgggcgttgt ttcgccggaa cggaaacttg cagaccgaaa tgccagccgc
      841 atgctgattc tgttgtgggt gcttctcgcc ggatatgcgg ttggtatcat attgccgctc
      901 attctgcgac ggccgcagcc acagagtctc gccgccagca tctgtgcaat tgtggcgacc
      961 ggttcgggca ttgccctggg actgctcggt ctcagggttt ctgaaccggt caccttctcg
     1021 ctcggatcga cgattcccct gctcaccttt gcgattcggc tcgacgccct cgcagcgttc
     1081 tttgttttaa ccatctccct cgtgggcctc gccgcctcga tctacgccat cgggtatctg
     1141 cggcactttc acggacgcgt gtcggttgcc gcgctgggag cgctcttcaa tgcgttcctc
     1201 tgctcgatga ccctggtcgt catcgctgac aacggatttt tcttcctgat cgtctgggaa
     1261 ctgatgtccc tgacctccta ctttctcgtc gtcacggagc atgagaaagc ggacgtgcgc
     1321 tacgcaggct tcttctacct gatcatgacg catgtcggca cggcgtttat cattgtggcg
     1381 tttctcctgt tcttccaaca gggggcgtcg ttctcgttcg acgcatttcg tcaccccgag
     1441 cagccgttag cggaaaatct gcggacattc gcgttcctgg ccgctctgat cggctttggc
     1501 accaaggcgg gggtcgtccc gttgcacgtg tggctcccct acgcccatcc ggcggcgccg
     1561 tcacatattt ccgccctcat gtccggcgtg atgatcaaga cggccatcta cggcctgctc
     1621 cgggtcacat tcgactttct cgacggacag tttccctggt ggtggggctt cgcagtgctc
     1681 accatcggcg cggtctccgc attgctgggc gtgatgtatg cgctgatgga acatgacctc
     1741 aagagcctgc tggcgtatca cagcgtcgag aacatcggga tcattctgct cggcatcggc
     1801 gccggcatga tctttcacgc ctatggactc aacgagctgg ccgcactcgg attagtggcg
     1861 ggcctgtatc acacgatcaa ccatgccatg ttcaaggccc tcctgtttct cggcgcaggg
     1921 tcgctgctct atgcgaccca tacgcgcaac atggaggaat atggcgggtt gctgcgccgg
     1981 atgccctgga caggattctg ttttctcatc ggcgcggtct ccatctcggc gcttccgccc
     2041 acgaacggat tcgtcagcga atggctggtc tttcagagtc tcttcttaag ctttcaggtg
     2101 ccctcgttgc tggtgaagct catgctcccg ctcgccgccg ccatgttggc gctcaccgga
     2161 gtgttagccc tggcctgttt cgccaaggca ttcggcattt cgttcctggc gttgccccgc
     2221 agctcccacg cacgtcacgc ggaagaagtg ccctggtcca tgcgcatcgg gatgggcctg
     2281 ctctccgtca tctgcattct cctgggactg gctcccatga tcgtggtgcc gatgttggac
     2341 cggatcacgt ccccgatggc cggcgcctcg atcgcgaaca aaatgctggc gctcgatggt
     2401 tgggccctgg cccccgtcaa tgtggagttt tccagtctgt cagccccggt gctcgctctc
     2461 gcgcttgtcg ggacggcggc agtggccctc gccctcgtcg cgctggcggg cggctttcgc
     2521 gcgaaacggt atttcaaaac ctggggatgc ggcctcaacc tgacgcctcg catggaatac
     2581 acggccacgg ggtttgtgca gccgatcaaa cgggtgttca gcacaatcta ccagccgaca
     2641 gtcaaactcg aaaccgagtt ccttgaacaa tcgaagtatt tcgccaaaca tcgccgtttc
     2701 gaatttcaca tcgaacccgt atttgaaacg tatctctatc ggccggtcgt cgcgttcgtg
     2761 tccatg
//
LOCUS       DATIFQ010000177         3494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2550469, whole genome shotgun sequence.
ACCESSION   DATIFQ010000177 DATIFQ010000000
VERSION     DATIFQ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550469"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1357)
                     /locus_tag="VLE22_07910"
     CDS             complement(50..1357)
                     /locus_tag="VLE22_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14369.1"
                     /translation="MLVRLSRTLILVALVALIAAPGASGQEVKKNVKDRAEYDLYVAI
                     SKEADNNKKLQLLNEWKQKYPATDFKEERLEFYLFTYQGLNRAADMYETAKELVALNP
                     KNIRGLSWVLVLTPTLGKNTPDILDTGEKAANSLLSSIDTLFAASAKPAATSEADWTK
                     ARTDSESLAHKTLGWVAMQRKDYAAAEKQFKESLKDNPNAGEVSYWLGTVLVSQRVAE
                     KQAEALFHFARASTYEGPGALSAQGRAEINKYFEKVYNSYHGSAEGIDALRQAAKTSA
                     TPPEGFKIKSSYEVALEKEEELKRTNPMLALWLNVRSELTGANGPQYWEGQVKGAGLP
                     GGVSGVSKFKGKLVSHAPAKNPKELVLAISDASTPEVTLKLDEAMAGAADPGTEIEFE
                     GVAAEFSKDPFMLTFDVEKAKVSGWPAPVKKAPAAKKAVRKKK"
     gene            1588..3048
                     /locus_tag="VLE22_07915"
     CDS             1588..3048
                     /locus_tag="VLE22_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HSB14370.1"
                     /translation="MRYLDLGVIVAYLIGITWFGAHFRHGQRTLKDYFLGGRVAPWWA
                     IAFSIVSAETSTLTVIGTPALAFSGNLGFLQVVFGYLLARVLISIIFLPHYFRGEMYT
                     AYELMQVRFGPRIRKLTAGTFLVLRALAEGVRVFAISIVISIILGTGEFVSIVLIVCL
                     TLFYTFEGGMTAVIWTDVVQMVLYIAGALVSFFVILDKIPGGWDHVWSVASSAGKFQI
                     FDFGFDLSGEFFARTYSFWAGILGGCFLTTASHGTEQLMVQRLLSARSQNESRMALLG
                     SWVVIFFQFSLFLVIGVILYVFYRQSGSPPPRPADRIYPEFVWNYLPPGVSGLVIAAI
                     LAAAMSNLSAALNSLASTSIMDFYKPMVARRGGLGHSEAQYLRLARGATVGWGAVLLL
                     IGWLARKWGSVLEAGLSIASILYGSLLGVFLLGLLTKRVGERAAIAGMLAGLITMWYV
                     RSSTNIAWTWYVLIGTSATLAVGLLSSFLIREQRDA"
     gene            3041..>3494
                     /locus_tag="VLE22_07920"
     CDS             3041..>3494
                     /locus_tag="VLE22_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB14371.1"
                     /translation="MPERVTEDGLRRVLGLPSAVAIVVGTVIGSGIFLVPKAMVLHVG
                     STRLVFAVWVAGGLLSLFGALSYAELAAALPEAGGEYVYLREAYGPLWGFVFGWTQTW
                     VAKSGSIATLATAFFYYLANFLPGLEKTLALIPLPVGPGGGPLEIRYGQ"
BASE COUNT          533 a         1084 c         1017 g          860 t
ORIGIN      
        1 agcctggtcc tggcgcggtg ccaggaccag gctgaaccga aaaccggggt cacttcttct
       61 ttctgactgc tttctttgct gcgggagctt ttttcacggg agccggccat ccgctaactt
      121 tcgccttctc cacgtcgaac gtcagcatga atgggtcctt cgagaattcg gcggcgactc
      181 cttcgaactc gatctcggtg ccggggtccg cagccccggc catcgcctca tcgagcttca
      241 gcgtgacctc gggcgtggac gcgtcgctga tcgccagaac cagttccttc gggttcttgg
      301 ccggagcgtg agacacgagc ttgcctttga acttcgagac gcccgaaacg ccgcccggca
      361 gcccggcgcc cttcacttgg ccttcccagt actgcggacc gttcgctccg gtgagttccg
      421 aacggacgtt cagccagaga gccagcatcg ggttcgttcg cttgagttcc tcttccttct
      481 ccagcgcaac ctcgtaagaa ctcttgatct tgaagccctc cggcggcgtg gcgctcgtct
      541 tggcagcctg acgaagtgca tcgatgccct cggccgaacc gtggtatgag ttgtagacct
      601 tttcgaagta cttgttgatc tcggcgcggc cctgagcgct caatgcaccg ggaccctcat
      661 acgtggaggc gcgggcgaag tgaaagagag cctcggcctg tttctccgcc actctctggg
      721 acaccagcac cgtgccgagc cagtaggaga cctccccggc attcgggttg tctttcagac
      781 tttctttgaa ttgtttctcg gcggcggcgt aatccttccg ttgcatggcg acccagccga
      841 gggttttgtg cgccagtgac tcgctatcgg ttcgcgcctt cgtccagtct gcctcactcg
      901 tagcggcagg ctttgcgctg gcggcgaaca gcgtgtcgat gctggaaagc agactgttgg
      961 cggccttctc tccggtgtcg aggatatcgg gggtgttctt cccgagtgtc ggcgtcaaca
     1021 ccagcaccca cgaaagcccg cggatgttct tcgggttgag cgcgaccagc tccttcgcgg
     1081 tctcgtacat gtcggccgcc cggttcaaac cctggtaggt gaacaggtag aactccaggc
     1141 gctcttcctt gaagtcggtg gcggggtact tctgcttcca ctcgttcaga agttggagct
     1201 tcttgttgtt gtcggcctct ttgctaatag caacatagag gtcgtactct gccctgtctt
     1261 tcacgttctt cttgacttcc tgccccgacg ccccgggcgc cgctattagg gcgacaagcg
     1321 cgactaggat caaagttctc gagagtcgta ccagcactct ggcctcctgg actgccctgt
     1381 gaatttcaca tccgccgggc tttcgcgccg gcagcaccat ctcgattatg tctcccattg
     1441 cacgccggaa ccgtccatcg caaacgtgca attgcggagt atagtccgaa tcgggcatcg
     1501 cctgcaacct ttagatgccg ctgagtgaaa gaatgtttcc atatccggct gcctcgtctt
     1561 gggggtgtgc tagaatcccc ctttcccatg cgctacctcg accttggggt tattgtcgcc
     1621 tacctcatag gaatcacctg gttcggcgcg catttccgtc acggccagcg cacccttaag
     1681 gactacttcc tgggcggccg ggtggctccc tggtgggcta ttgccttctc catcgtctcg
     1741 gctgagacga gcaccctgac ggtcatcggc acccccgccc tcgccttctc cggaaacctt
     1801 ggctttcttc aggttgtctt cggctacctg ctcgcgcggg ttctgatctc gatcatattc
     1861 ctcccgcact atttccgggg tgaaatgtac acagcctacg agctgatgca ggttcgtttc
     1921 ggaccacgca tccgcaagct caccgccgga accttcctcg ttctccgcgc gcttgcggaa
     1981 ggtgtccgtg tcttcgcgat ctcgatcgtt atctcgatca tcctcggcac cggcgagttt
     2041 gtctcgatcg ttctgatcgt ctgcctcacg ttgttctaca cgttcgaagg cgggatgacg
     2101 gcggtgattt ggaccgatgt cgtccagatg gtgctctaca tcgccggcgc tctcgtcagc
     2161 ttctttgtaa ttctcgacaa gattcccggg ggttgggacc acgtttggag cgtggcctcg
     2221 tcggccggga agttccagat ctttgatttc ggctttgacc tgtctggcga gttcttcgcc
     2281 cgcacttatt cgttctgggc cggaatcctc ggagggtgct tcctcaccac cgctagccac
     2341 ggcacggaac agctcatggt gcagcgtctg ctgtccgcgc ggtcgcagaa cgagagccgc
     2401 atggccttgc tcggaagctg ggtcgtgatc ttttttcagt tcagcctgtt cctggtcatc
     2461 ggagttatcc tctacgtctt ctaccggcag tctggctcac ccccgccacg gccggccgac
     2521 cggatctatc ctgagtttgt ctggaattac ctgccgcctg gcgtctccgg gctcgtgatc
     2581 gcggcaattc tcgccgccgc gatgtccaac ctcagcgccg cgctcaactc cctcgcttcg
     2641 accagcatca tggacttcta caagcctatg gttgcccgcc gcggaggact gggccattct
     2701 gaagctcaat accttcgatt ggcgagagga gctacagtcg gctggggcgc cgttctgctt
     2761 cttattggtt ggctggctcg aaagtggggc tcggtgctag aggccggtct ctcgatcgcc
     2821 tctatcctct acggttctct cctcggagtg ttccttttgg gtttgctgac gaaacgggtc
     2881 ggagagagag cggcgatcgc gggcatgctc gccggactga tcacaatgtg gtacgtgcgg
     2941 agttccacca atattgcctg gacgtggtac gtgctgatcg gcacgtccgc gacgctcgcg
     3001 gtcggtctat tgtcgtcatt tctcatcagg gagcagcggg atgcctgaac gggtcacgga
     3061 agacgggctg cggcgggtcc tgggcctgcc cagcgccgtc gcgattgtag tcggcacagt
     3121 catcggcagc gggatttttc ttgtaccgaa agcgatggtt ctgcatgttg gctccaccag
     3181 gcttgttttt gcggtctggg tcgccggcgg cctgctgtcg ctcttcggag ctttaagcta
     3241 cgcggagctg gcggcggccc ttcccgaagc cggcggcgag tacgtatacc tgcgtgaggc
     3301 gtacggcccc ctctggggtt tcgtctttgg ttggacccag acgtgggtcg caaagagcgg
     3361 ctcgattgcg accttggcga cggctttttt ctactatctg gccaatttcc tgcccggtct
     3421 cgagaaaacc cttgcgctca ttcccttgcc cgtcggacct gggggaggtc cactggagat
     3481 ccgctacggc cagt
//
LOCUS       DATIFQ010000178         3817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_59707, whole genome shotgun sequence.
ACCESSION   DATIFQ010000178 DATIFQ010000000
VERSION     DATIFQ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59707"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VLE22_07925"
     CDS             complement(<1..206)
                     /locus_tag="VLE22_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14372.1"
                     /translation="MLEFNSILVFSGDPKKLADFYKKVFQKDPDWSEGGYYGFMAGKG
                     FITFGPHDKVQGKNANPERVMFNF"
     gene            336..1475
                     /locus_tag="VLE22_07930"
     CDS             336..1475
                     /locus_tag="VLE22_07930"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HSB14373.1"
                     /translation="MPIIATIGGIGLIAVVLWEGFETIVLPRRVTRRFRLTRFFYRRT
                     WRPWSKMVGSVVPGRWQETWLSVFGPLSLLLLLSIWAGGLITGFALLHWAAGSAVLAR
                     EGSYGFLTDLYLSGTTFFTLGIGDVVPRSAVARILVVIESGMGFAFLALVIGYLPALN
                     QSFARREVSISLLDARAGSPPTAAEMLLRHGHERGMEALRQLLHEWERWSAEFLEGHL
                     SYPVLAYFRSQHDNESWLAALTAILDTCALVMAGLEGACERQAELTFAIARHAVVDLC
                     LVFRTGPREPKHDRLPPETLAELRATLATSGIKLHEGEAADLKLEELRGMYEPYLYAL
                     STYFQVGIPPWIAVERRPDNWQGNISAAEGAEMNAARGRGKGDHF"
     gene            2159..2281
                     /locus_tag="VLE22_07935"
     CDS             2159..2281
                     /locus_tag="VLE22_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14374.1"
                     /translation="MRLMTIVKAARDSEADVMPEVKLFAEMAAYHEELAKAGAR"
     gene            2289..>3817
                     /locus_tag="VLE22_07940"
     CDS             2289..>3817
                     /locus_tag="VLE22_07940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB14375.1"
                     /translation="MPRAGRKEWVGLAVIALPCMLYSMDLTVLNLAVPSISAELAPSS
                     AELLWIVDIYGFLVAGSLITMGTLGDRIGRRKLLLIGAAVFGAASVLASFSTSAPMLI
                     ATRALQGVAAATLAPSTLSLIRNMFHDPRQRTVAISAWIMSFSAGAAIGPLIGGVLLE
                     HFRWGSVFLINVPVMLLLLAIGPVFLPEFRDPEAGRLDPVSAVQSLAAVLTAIYGLKR
                     IAENGFGWIPLLSILLGIAVGVAFVRRQRRLADPLIELRLFRIPAFSAALAVNILAFN
                     TAFATFLFIAQYLQSVLGLSPLAAGMWGVPSALAFVVGSMLTPAIVGRFQPAHVVAGG
                     MAFSSAGFFLLTLVDNVASALPVLVAGSVVFSLGMSPVVALTTDIVLGAAPPERAGAA
                     SALSETSSEFGGALGIAILGSIVTAIYRGKVAAALPAGISTEAAEAARSTIGGAVSVS
                     GQLPGTLGADLLGSAREAFVQAFGTTAAISAAIAMGVAILAATLLRQPRAHAVKASAR
                     E"
BASE COUNT          619 a         1243 c         1199 g          756 t
ORIGIN      
        1 tcaaaattga acattaccct ttcggggttc gcgttcttgc cctggacttt gtcatgcgga
       61 ccaaaggtga tgaacccctt gccggccatg aatccgtaat atccgccttc cgaccagtcg
      121 gggtccttct ggaaaacctt cttgtaaaaa tccgctagtt tcttgggatc cccggaaaaa
      181 accaggatgg aattgaattc cagcatgatc ctcgcctcct tttcacggcg atcggttttc
      241 ctttagatga gcggtccaac gaaaggttgg aggcggtcga agctggagcg cgacctcgtt
      301 aaggagccgg ccatctaaac atttcggagg cgtatgtgcc gatcatcgcg accataggcg
      361 gcattggcct gatcgcagtg gtcctatggg aggggttcga gaccatcgtc ctgccgcggc
      421 gtgtgacccg ccgtttccgc ctcactcggt ttttctaccg gcgcacctgg cggccatggt
      481 cgaagatggt cggctccgtc gtcccgggta gatggcagga aacctggctg agcgtgttcg
      541 gcccgctgtc actcctcctg ctcctgagca tctgggccgg ggggctgatc acaggctttg
      601 ccctcctcca ctgggcagcc ggctctgccg ttctggcccg ggaagggagc tacggatttc
      661 tcaccgacct gtacttgagc ggcaccacct tctttacgct cgggatcggg gacgtcgtcc
      721 cgcggagcgc cgtggcgcgc atactcgtgg tgatcgaatc ggggatgggg ttcgcgttcc
      781 tcgccctggt gatcggctac ctcccggccc tcaatcagtc cttcgcccgc cgcgaggtga
      841 gcatctcgct tctggatgcc cgcgccggct cccctcccac cgccgccgag atgctcctcc
      901 ggcacggcca cgagcggggg atggaggcgc tccggcaact cctccacgaa tgggagcgtt
      961 ggtcggcgga gttcctcgag gggcacctct cctatccggt cctcgcgtat ttccgttccc
     1021 agcacgacaa cgaatcgtgg ctggcggctt tgacggcgat cctcgacacc tgcgcactgg
     1081 tcatggccgg cctggagggg gcctgcgagc gccaggcgga actcaccttc gcgattgccc
     1141 gccatgccgt ggtcgacctc tgcctggtct tcaggaccgg gccgcgggag ccgaaacatg
     1201 accgcctgcc gccggaaacg ctggccgaac tacgggccac cctggcgaca tcgggcatca
     1261 agctccacga gggggaagca gccgacctca agttggagga gctaaggggg atgtacgagc
     1321 cgtatcttta tgccctgtcc acgtacttcc aggtcggcat accaccctgg atcgctgtgg
     1381 aaaggagacc ggacaactgg caggggaaca tctcggcggc ggaaggcgcc gagatgaatg
     1441 cagcccgtgg aagaggaaaa ggggaccatt tctaaacaaa cagctcattt cgcggaattc
     1501 tccaggtgtc ccccgaactg aaaccgcatt gccgacagca gcttgtcctg gaaatccgcc
     1561 accccgcgcg aggcgaatcg ctcctccacc ttccggaggt cggctacttc ggtcagatcc
     1621 gccgccagta cctccgccga taccccatgg tctgcatgtt gccctatgta tttaatgcgg
     1681 tctggtttga gttcggctcg caggggcctg catccgctcc gatgcccgcc gctccgcatc
     1741 ctcacgggct cgacgtgatc cgatcacgca ggaatcgcaa ggatgtctgg aggcgacgaa
     1801 cgcgcctctc gcctcttccc gtgtcggggt cgtccgtcga tcgaggcatg cgactgcgtc
     1861 ccgttcgtaa tcgcactgtg acatggcatc tccctcccat gccgtataga gcgaccgatc
     1921 ccggattcgg ttccctcgaa tttctgcctg cctcctcacc atgctcgaga tgacttgaag
     1981 tcacacgctt cagcctcaag tagagggatt ggacgatccc actttacagg gagaacccgg
     2041 ggaatcctcc gaagaaccac ggcatccgaa tatcttatga ccgcatgtcg aattcagacg
     2101 tcctggaacg actaagaggc agatggacaa ttctgcaacc atttaaagga gaccaaagat
     2161 gcggctcatg accatcgtca aggccgccag ggactcggaa gccgacgtca tgcctgaggt
     2221 aaagctcttc gccgaaatgg cggcgtacca cgaggagctg gcgaaagcag gcgcgaggta
     2281 ggagcgcgat gccacgggcc ggacgcaaag agtgggtcgg gctggcggtg atcgcgctgc
     2341 cgtgcatgct ctattccatg gacctgacgg tgctgaacct ggcagttcca tcgatcagcg
     2401 cggagcttgc accgagcagc gccgagctgc tctggatcgt ggatatctac ggattcctcg
     2461 tcgccggatc gctcattacg atgggcacgc tcggcgaccg gatcggccgc cgcaagctgc
     2521 tcctgatcgg cgcggcggtg ttcggcgcgg cctcggtgct cgcctcgttc tccacgagcg
     2581 cgccgatgct gatcgccacc cgcgccctgc agggagtcgc cgcggcgacg ctggcgccgt
     2641 cgaccctgtc cctgatccgg aacatgttcc acgatcctag gcagcgcacg gtcgccatca
     2701 gcgcgtggat catgagcttc tcggccgggg cagcgatcgg ccccctgatc gggggcgtgc
     2761 tgctcgagca tttccggtgg ggctcggtgt tcctgatcaa cgttcctgtg atgctgctgc
     2821 tcctcgcgat cggcccggtc tttcttccgg agttccggga tccggaggcg ggccggctcg
     2881 acccggtgag cgccgtccaa tcgctggcgg ccgtcctgac ggcgatctac ggtcttaagc
     2941 gcatcgcgga gaacggtttc ggctggatcc ccctcctgtc gatcctcttg gggatcgcgg
     3001 tcggcgtggc gttcgtgcgg cgccagcgaa ggcttgccga tccgctcatc gagctcaggc
     3061 ttttccgcat tcccgcattc agcgcggcgc tggccgtgaa catcctcgcc ttcaacacgg
     3121 cattcgccac gtttctcttc atcgcgcagt acctccagtc ggtgctgggc ctgtctccgc
     3181 ttgcggcggg catgtggggg gtcccctccg cgctggcgtt cgtcgtcggc tcgatgctta
     3241 cgcctgcgat cgtgggccgc ttccaacccg cgcacgtggt cgcgggcggc atggcgttct
     3301 cctccgccgg attctttctg ctcacgctgg tagataatgt cgcgtccgcg ctgccggtcc
     3361 tggtggccgg atcggtcgtg ttctcgctcg gcatgtcccc ggtggtcgcg ttgacgaccg
     3421 acattgtcct gggggccgcc ccgccggagc gcgccggcgc cgcgtcggcg ctgtcggaga
     3481 ccagcagcga gttcggcggg gcactgggca tcgcgatatt gggcagcatc gtgaccgcga
     3541 tctatcgcgg caaagtagcc gccgccctgc ccgcaggcat ctccacggag gcagccgagg
     3601 ccgctcgcag cacgatcgga ggggcggttt cggtttcagg acaactgccc ggcacgctcg
     3661 gggccgacct cctcggatcc gcccgggagg cgttcgtaca ggcgttcgga acgacggccg
     3721 ccatcagcgc ggcgatagcg atgggggtcg cgatccttgc cgcgaccctt cttcggcagc
     3781 cccgggcgca tgccgtgaaa gcatccgccc gggagca
//
LOCUS       DATIFQ010000179         8964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1209729, whole genome shotgun sequence.
ACCESSION   DATIFQ010000179 DATIFQ010000000
VERSION     DATIFQ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209729"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1856
                     /locus_tag="VLE22_07945"
     CDS             72..1856
                     /locus_tag="VLE22_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB14376.1"
                     /translation="MTRRKLLSLAALSGAWARAQEFGGMASRGVKPLPRPKPSGLPFH
                     CRFTDVATQAGLKDPVIYGGLNSNAYIIEAMGSGIAFFDFDNDGWLDVLVLSGTRWEG
                     TPGATNRLYKNNRDGTFSDVTEKAGLTRAGWAYGVTIGDFNNDGFEDVFLTYWGQNVL
                     YRNNGDGTFTDVTKQAGLLQPSSRWGSGCTWIDYDRDGLLDLFVSNYLVFDPKTVPAA
                     GVSPNCNFKGILINCGPRGLRPETFMLYHNNGDGTFTDVTAKSGVGKIKGSFGLTAVA
                     ADFDNDGWPDIFVAGDSTPSLLFRNNHDGTFTEEGLERGVALNEDGQEQAGMGIGVGD
                     FDLDGNLDIFKTHFTEDTPALFRNNGKGNYRDVTIRSGLGVETRFVSWGTGIEDLDND
                     GAPDIFWVTGSVYPEVGLKHPDFPHRTPRILFRNLGNGKFEQLFDEAGPDLNAPHASR
                     GVAFGDFDNDGDLDILIMNQNEPPTLLRNDVTGDHHWLKVKLIGVKSNRSAIGAQVIA
                     AYGERKHAKAVTAQSAYLSVSDSRLHFGLGQEKTADLEIRWPNGSVEKVAAVSADQLV
                     VIKEGSGVVDKLKFSTALSSRRERPKQP"
     gene            complement(1821..5045)
                     /locus_tag="VLE22_07950"
     CDS             complement(1821..5045)
                     /locus_tag="VLE22_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HSB14377.1"
                     /translation="MRFRNLQRVGAFCAVAVVAVVLLQSADRAEVNQEQLWRHRNLGK
                     AFYENPTTLAQSVDEFRKALALAPESVRERVNYGLALLRTNSILEGVAELKKAQKQEP
                     KLPHTWFNLAIAYKKLRQYDKAVEQFQGMLRLVPDDPISHYNLGLIYKLQDRVDDALR
                     EFEITSKLNPDLVAPRFQTFNTYRELDRAEQAEKALAEFRAAKERQAGFDESEDMEWN
                     WYAEVYDPTDAAAEAVPAAGELQFAERMLAGGADPKTAGMASLHSDGGQRLDLLVWSV
                     RGIQLYRGGTDLATESGLAAAGGVLSAAPGDIDNDGLPDLCVLTSSGAFLYRNDKGTF
                     RKPEAPLAAGVFRKAVWLDYDHDYDLDLLLLGDKSVLLRNQGPAGFTDRTADFPFVAG
                     RALDAAVIHLVPDTREIDLAVSFSDREGVLYHDRLLGRYEATPLEGLTKGASSLQQAD
                     IDLDGSMDLVFQSGAGVTLLRNRNGRFESPIAMVTGRGPVRFADFEDRAAPDLVAGGS
                     VFRNLGVGKFEAEAARKLTDASALAAGDFNLDGRIDVAAAGADGSVRLYLNETATTQQ
                     WAGIRLSGVKNLKVPVFAEVEIKSGALYQKQMYDGSALVFGLRSHPKIDTVRITWANG
                     LVQNQTGEAAGRYLDIVEAPRLSGSCPMIFTWNGRRFEFITDVLGVAPLGARSGDGRF
                     FATDHDEYVQIPGESLVPKDGRYEIRVTEELSEVSYLDKVQLIAVDHSAQVEVYSNEK
                     WKSPPYPEFRLFGVERRVYPRAARDHRGRDVLGRITRRDGVYADTFVRDHAGTAETHA
                     IDLDFSGAAPANRAVLVLNGWVDWADGSVFLAQSQQGGRGLTPPYLQVKDTAGQWRTV
                     IEDMGMPSGKTKTVAVDLSGKFLSRSREVRIVTNMCVYWDEIFLSEDSAAPQARLTPL
                     DAKRADLRFRGFSKVYVHPERRQPERFEYHDVRLASQWNPTPGYYTRYGPVEELVSEE
                     DDRLVVMGSGDELALSFTTNDLPPLPAGWKRDFLLLVGGWAKDRDANTAWSQTVEPLP
                     FHGMSSYPFAEGERYPCSARLEEYRRTYNTRPALRLLGSLAPGR"
     gene            complement(5123..7402)
                     /locus_tag="VLE22_07955"
     CDS             complement(5123..7402)
                     /locus_tag="VLE22_07955"
                     /inference="COORDINATES: protein motif:HMM:NF027656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HSB14378.1"
                     /translation="MVRLSAVAVFLVCLLAGAVGVSDRPPGQDDIGYLPREGATVATN
                     PPALAWLPEPGAEGYAVQFARDEGFSRGVIEVPRTPYVLYTHTRTLDSGTWWWRYACV
                     DKEGSRSNWSRARSFTIAGDAQPFPRPDEDLIRERMPKQHPRLLLRPESVGELRKARL
                     GGHKQRWDQLAEAAENALRMPLIPEPPEWTGGKWNAEEWRRNFLEAIKASEAAETLAF
                     CYMLTGEPRYGEGARKWLLHIASWKPLGTTSMDVNDEAGMPILYLTSRAYDWAYDALS
                     EADRETMRRMVLARGEEAYHWLHDKPHEQKAYNSHAGRMWHFLGEAAIAYYGEVPEAK
                     KWLDYALTIFWGWYPAFGDEDGGWAQGYSYWASYVNRSTWWLDALNAALGVDGTQKPF
                     YRNVGHFPMYVSPPGGALTGFGDFAERRPNAEPAAVVQFFARARQVPEWQWYAEAWGK
                     GEGASGPIGFIRAARTEPPPVKSRRPADWPFAKLFRGAGWAALHSNLLDGKDDVQVMM
                     RAAPLGNISHSHADQNAIVLGAYGSPLLVNTGLRPWYGSPFCEQWYWTTKAHNALEID
                     GKGQPKTAAGKGEVIVFRPGRQYDYVVGDASTAYGANVERYRRHLVFLKPNVLVVVDE
                     VKGKQPVSLKVWLHGRAPFTMNEEAGAISLEYENAALAGRVLAPGGLRFEQTDKYTIP
                     PEMGKPEPEWHFSAETKEPQTSVRAVAVLAMAKAGHRPELEQAEDVSRGSFIAVRFRH
                     GGRVRTVTIDTAGPTLKVE"
     gene            complement(7567..>8964)
                     /locus_tag="VLE22_07960"
     CDS             complement(7567..>8964)
                     /locus_tag="VLE22_07960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB14379.1"
                     /translation="GAVRTVTGSMHEVQVNGRRFLLDCGLYQGRRKDANDRNRSFPFS
                     PKSVDAVVLSHAHIDHSGNLPSLVKQGFEGPIYATPPTADLCDAMLRDSAYLQEKDAA
                     YLNKRHHRRKALDPELTNGPVEPLYTIEDAERALKQFRQVPMYTPTRLDDLLSYESYN
                     AGHMLGSTAVLLHYHENGKRIRLAFSGDVGRPKQAIVRDPDPLENVDYLIMETTYGDR
                     LHKELGAVSAKLAEIVNRTAARGGKIIVPSFAVGRTQQLVLLLHELTNARKIPRIPVF
                     VDSPLAVDATKIFQRHSECFDEETRQYLLEGEDPFGFQTLRYVRDASESKTLNDLRGP
                     FMVISASGMCEAGRILHHLRNNIEDPRNTVLITGFQAENTLGRKIVDKMQEVPIFGEP
                     MRLRAEVAKLNELSGHADQRELLLWLKPMVAGLKKIFLVHGEPAQTAAFAKAVEQQYG
                     LETVMPERGQSFELG"
BASE COUNT         1610 a         3133 c         2532 g         1689 t
ORIGIN      
        1 gcctctctcg gtcgatgtac gcgcattgtc gcgtgtcaca gtccttcagt taatatgaaa
       61 cagacggggc gatgactcgg cggaaactgc tctctctggc ggcgctctct ggcgcttggg
      121 cgcgcgcaca agaattcggc gggatggctt cacggggtgt gaagccgttg ccccggccca
      181 aaccgtctgg cttgccgttt cactgccgct tcaccgacgt cgccacccag gctggtctta
      241 aggaccctgt catctatgga ggcttgaact ctaacgccta catcattgaa gcgatgggct
      301 cgggaatagc cttcttcgac ttcgataacg acggctggct cgacgttctc gtgctatcgg
      361 ggacacgctg ggaaggcact cctggagcca cgaatcgcct ttacaagaac aaccgcgatg
      421 gcactttttc cgacgtcacc gaaaaggccg gcctcacgcg cgccggctgg gcctacggcg
      481 tcacaatcgg cgacttcaac aacgacggct tcgaagacgt gttcctgacc tactggggac
      541 aaaacgtcct gtatcgcaat aatggcgacg gcaccttcac cgacgtaacc aaacaagccg
      601 gccttctcca accctcctcc cgatggggtt ccggctgcac ctggatcgat tacgaccgcg
      661 acggactgct ggacctgttc gtctccaact acctagtctt cgacccgaag accgtccccg
      721 ccgccggcgt cagcccgaac tgcaatttca agggcatcct aatcaactgc ggtccgcgcg
      781 gcctccggcc ggagaccttc atgctctacc acaacaacgg cgacggcacc ttcaccgatg
      841 tgactgccaa gtccggcgtg ggcaagatca agggtagttt cggactcacc gccgtcgcag
      901 ccgacttcga caatgatgga tggcccgaca tcttcgtcgc cggcgattcc acgcccagcc
      961 ttctgttccg caacaaccac gacggtactt tcaccgaaga gggactggaa cgcggcgtcg
     1021 cccttaacga ggacgggcag gagcaggccg gcatgggcat tggcgtcggg gatttcgacc
     1081 tcgatggcaa cctcgacatt ttcaagaccc acttcaccga ggacacgccc gccctgttcc
     1141 gcaacaacgg taagggcaat taccgggacg tcaccatccg ttcgggcctc ggcgtcgaga
     1201 cccgctttgt aagctggggc accggcatcg aggatctcga caacgacggt gcgcctgaca
     1261 tcttctgggt caccggcagc gtctatcccg aggtgggact caagcacccc gacttcccac
     1321 accgcacacc ccgtattctg ttccgcaatc tcggcaatgg aaagttcgag cagttgttcg
     1381 acgaagccgg gccagacttg aatgcgcccc atgcaagtcg cggcgtggcg ttcggcgact
     1441 tcgacaatga cggcgacctc gacatcctca tcatgaacca gaacgagccg ccaaccctgc
     1501 tccgcaacga cgtcaccggc gaccaccact ggctgaaagt gaaactgatc ggtgtaaagt
     1561 cgaaccgcag cgccatcggt gcgcaggtga tcgccgccta cggcgagcga aaacacgcga
     1621 aagccgtcac ggcccaatcg gcttacctgt cggtgagcga cagccggctg cacttcggtc
     1681 tcggccagga aaagaccgcg gacctcgaga tccgctggcc caacggcagc gtggagaaag
     1741 ttgccgcagt ttcggcggat cagctcgtcg tcatcaagga aggctccggc gtcgtggaca
     1801 agctcaagtt cagcaccgcg ctatcgtccc ggcgcgagcg acccaagcag ccgtaacgcc
     1861 ggccgtgtat tgtacgtccg ccggtactct tcgagccttg cgctgcacgg atatcgctca
     1921 ccctccgcga acggataact cgacatcccg tggaacggca gcggctcgac cgtctgcgac
     1981 caggccgtgt tcgcgtctct atccttcgcc cagccgccca ccaaaagtag aaaatcccgt
     2041 ttccagccgg ctggaagcgg tggcagatca ttcgtggtaa acgacagtgc cagctcatcc
     2101 cccgaaccca tcaccaccag ccggtcgtcc tcctcggata ccagttcctc aaccggaccg
     2161 taccgcgtgt agtaaccggg cgttgggttc cactgcgagg cgagccggac gtcatgatat
     2221 tcgaaccgct ccggctgcct tcgctccgga tgcacgtaaa ccttcgagaa accgcggaaa
     2281 cggagatcgg cccgcttcgc gtcgagcggc gtcaatcgcg cctgcggtgc cgcgctgtct
     2341 tcgcttagaa agatctcgtc ccaatacacg cacatgttcg tcacgatacg aacctctcgc
     2401 gaccgcgaca gaaactttcc gctgaggtcg accgcgacgg tcttcgtctt gccggacggc
     2461 atgcccatgt cctcgatcac cgtacgccat tggcccgctg tatctttcac ctggaggtag
     2521 ggtggtgtaa gccctctccc gccctgctgg gactgtgcaa ggaacacgct cccgtccgcc
     2581 caatccaccc agccgttcag caccagcacc gcccggttcg ccggcgctgc gccactgaag
     2641 tcgaggtcga tcgcgtgcgt ctcagccgtg ccagcatggt cgcgcacgaa cgtatccgcg
     2701 tacaccccat cccgcctcgt gatccggccg agcacgtctc tcccgcggtg gtcgcgcgcc
     2761 gcacgcgggt acacgcgccg ctccacgccg aacaaccgga actccggata cggcgggctc
     2821 ttccatttct cgttcgagta cacctcgacc tgggcagaat ggtccaccgc gatcagttgc
     2881 accttgtcga gatagctgac ttcgctcagt tcctcggtga cgcggatctc gtaacggcca
     2941 tccttcggca cgagcgattc gcccgggatc tgaacgtact cgtcatgatc ggtggcgaaa
     3001 aaccggccgt ctcccgatct cgccccaagc ggtgcgacac ccagcacatc ggtgatgaat
     3061 tcgaagcgcc gcccgttcca ggtgaagatc atcggacagg agcctgacag ccgcggcgcc
     3121 tcgacaatgt cgaggtagcg tcccgccgct tcgccggtct gattctgcac gagcccgttc
     3181 gcccatgtga tccgtacagt gtcgatcttc ggatgggacc gcaagccgaa gacgagcgcc
     3241 gagccgtcgt acatctgttt ctgatagagc gcgccgctct tgatctcgac ttcggcaaaa
     3301 actggaacct ttaggttctt cacgccggac aagcggatgc ccgcccactg ctgcgtcgtc
     3361 gcggtctcat tcaggtatag ccgaaccgag ccgtccgctc cggccgctgc cacgtcaatc
     3421 cgcccatcga ggttgaagtc cccagcggcg agcgcactcg catccgtcag cttccgcgca
     3481 gcctccgcct caaacttccc cactccgagg tttcggaaca ccgacccgcc ggcgacaaga
     3541 tccggcgcgg cccggtcctc gaaatccgca aaccgcaccg gcccacggcc cgtaaccatc
     3601 gcgatcgggc tctcgaacct gccgttccgg tttctcagaa gcgttacccc tgcccctgac
     3661 tgaaacacca ggtccatcga cccgtccagg tcgatgtcag cctgctgaag cgaggaagct
     3721 cccttcgtga gtccctccag cggcgtcgcc tcatagcggc caagcagacg gtcgtggtag
     3781 agcacgccct cacggtccga gaacgaaacg gcaaggtcaa tctctcgggt gtcggggaca
     3841 aggtggatca ccgcggcgtc aagcgcccgt ccggcgacaa acggaaaatc agccgtgcgg
     3901 tctgtaaaac ccgccggtcc ctgatttcgc aacagcacgg acttgtcgcc gagtagcagc
     3961 aggtctagat cgtaatcgtg atcgtaatcg agccaaaccg ccttgcgaaa cacgccggcc
     4021 gccagcggcg cctccggctt ccgaaatgtc cccttgtcgt ttcggtacaa gaatgcgccg
     4081 gaggaagtga gtacgcacag atcggggagt ccgtcgttgt ctatatcgcc gggcgcggcc
     4141 gacagcacac cccctgcggc ggctagacca ctctccgtcg ccaggtctgt gccgccgcgg
     4201 tacaactgga tgcctctaac cgaccagacc agcaagtcca gacgctgccc gccgtccgag
     4261 tgcagtgacg ccatcccggc ggtcttcggg tcggcgccgc ctgcaagcat ccgctcggcg
     4321 aattgcagtt cacccgcggc gggcacggcc tcagccgccg cgtctgtcgg atcgtacacc
     4381 tcggcgtacc agttccactc catgtcttcc gactcgtcga agccggcctg tctctccttc
     4441 gccgcgcgga actcggccag cgctttttcc gcctgctcgg ctcggtccag ttcccgatac
     4501 gtattaaagg tctggaaccg cggcgccacc aggtcgggat tcagcttgct cgtaatctcg
     4561 aattcgcgca gcgcatcatc gacgcggtcc tgaagcttgt agataaggcc gaggttgtag
     4621 tgcgagatcg ggtcgtccgg cacgagccgc aacattccct gaaactgttc caccgccttg
     4681 tcgtactgcc gcagcttctt gtacgcgatc gcgaggttga accacgtatg cgggagtttc
     4741 ggctcctgct tctgtgcttt cttgagctcg gcgacgcctt cgagaatgct gttcgtgcgc
     4801 agcagtgcca gcccgtagtt gacgcgctcc cggaccgatt ccggcgccag tgcgagcgcc
     4861 ttgcggaact catcaaccga ctgcgccagc gtcgtggggt tctcgtaaaa tgccttgcca
     4921 agattacgat gacgccagag ctgctcctgg ttgacctcgg cccggtcggc actctgaaga
     4981 agaaccaccg ccacgacagc gacggcgcag aacgctccta ctcgctgcag gttcctgaat
     5041 ctcaaaggtg gcacaggctt cagcctgtgg actctgattg ctttcacccg ttaccctcgc
     5101 cccgcaccag tctagcgaat ggtcactcca ccttgagtgt cggtcccgcg gtgtcaatgg
     5161 tcaccgttct cacgcggcct ccatgccgga accgaacggc gatgaaacta ccgcgcgaga
     5221 cgtcctcggc ctgctccagc tccggacggt gtcccgcctt tgccatcgcg agaaccgcca
     5281 ccgcgcgcac ggaggtctgc ggctccttcg tttcggccga gaagtgccac tcgggttccg
     5341 gtttccccat ctcgggcggg atcgtgtact tgtcggtctg ctcgaaccgc aagccgcccg
     5401 gcgccagcac acgccccgcc agcgccgcat tttcatactc caggctgatc gctcctgcct
     5461 cttcgttcat cgtaaacgga gcccggccgt gcagccagac cttcagcgac acgggctgct
     5521 tccccttcac ctcgtcgacc accaccagta cgttcggttt cagaaacaca aggtgccggc
     5581 gataccgctc gacgttcgct ccataagctg tgctggcatc tcccactacg tagtcgtact
     5641 gccgcccggg gcggaagacg atcacttctc ccttccccgc cgcggtcttc ggctggccct
     5701 ttccatcgat ctcgagcgcg ttgtgcgcct ttgttgtcca gtaccattgc tcgcaaaacg
     5761 gactgccgta ccacggccga agccccgtgt tgacaagcaa cggcgagccg taggcgccca
     5821 gcacgatggc gttctgatcg gcgtggctgt ggctgatatt gccgagcgga gcggcgcgca
     5881 tcatcacctg cacatcgtcc ttgccatcca gcaggttcga gtgcagcgcc gcccaaccgg
     5941 ccccgcggaa caatttcgca aacggccagt cggccggtct tctcgatttc acaggaggcg
     6001 gttccgtccg cgcggcgcgg atgaatccga tcggtccgct cgcgccttct cccttgcccc
     6061 atgcctccgc ataccactgc cattcgggga cctgccgcgc gcgtgcgaag aactgaacca
     6121 ccgccgcggg ctccgcattt ggacgtctct cggcaaagtc gccgaatccc gtcagcgcgc
     6181 cccccggcgg cgacacgtac atcggaaaat ggcccacgtt gcggtagaac ggcttctggg
     6241 tgccgtcgac gccgagcgcg gcattgagcg cgtccagcca ccatgtggac cggtttacgt
     6301 aactcgccca gtaggagtag ccctgcgccc agccgccgtc ctcatcgccg aacgccggat
     6361 accatcccca gaaaatcgtc agtgcgtagt ccaaccactt cttcgcctcc ggcacttcgc
     6421 cgtagtacgc gatggccgcc tcgccgagga aatgccacat ccggcccgcg tggctgttgt
     6481 acgccttctg ttcgtggggc ttgtcatgca gccagtggta ggcctcttcg ccgcgcgcca
     6541 gcaccatccg ccgcatcgtt tcgcggtcgg cttcgctgag cgcatcatac gcccagtcat
     6601 acgcccgtga cgtgaggtac agaatcggca tgccggcttc gtcgttgacg tccatactgg
     6661 tagtgccgag cggcttccac gacgcgatgt gaagcagcca cttccgcgcg ccctcgccgt
     6721 agcgaggctc gccggtcagc atgtagcaga acgcgagcgt ctccgccgcc tccgacgcct
     6781 tgatcgcttc aaggaaattg cgccgccact cctcggcgtt ccactttccg cccgtccact
     6841 ccggcggctc ggggatcagt ggcatgcgga gggcattctc cgccgcttcg gcaagctggt
     6901 cccagcgctg cttgtgtccg ccaagacgcg ctttgcgcaa ctcgcccacg ctctctggcc
     6961 gcaacaacag ccgcggatgc tgcttcggca tccgctcgcg gatcagatcc tcgtctggac
     7021 gcgggaacgg ctgcgcgtcg cccgcaatcg tgaagctccg ggcgcggctc cagtttgacc
     7081 gcgagccctc cttgtcgacg caggcatacc gccaccacca cgtgccggag tcgagcgtcc
     7141 gcgtgtgcgt gtagagcacg tacggcgtcc gcgggacttc gatcactccg cggctgaacc
     7201 cttcgtcgcg cgcgaactgg accgcgtagc cctccgctcc cggctccggc agccacgcga
     7261 gtgcgggcgg attggtcgcc accgtcgccc cctcgcgcgg cagatacccg atgtcatcct
     7321 gccccggcgg ccggtcggac actcccaccg cacccgccag taaacaaacc agaaacaccg
     7381 caacagcgct cagtctcacc atgatccgaa ccccgtcttc ggaaccgcga ccaccagcga
     7441 gcggccactt ccacccttaa ggaactgcga gcgtaagcga gcggtagcgc cagcgagcgg
     7501 cacgtcgccc ccaaggaacc gcgagcgtga gcgagcggca gcgccagcga gcggcaaccg
     7561 ctcctcctac cccaactcaa aactctgccc gcgttccggc atcaccgtct cgagcccgta
     7621 ctgctgttcc accgccttgg cgaatgccgc cgtctgcgca ggctctccgt ggaccagaaa
     7681 gatctttttc aatcccgcca ccatcggctt cagccacaga agcaactccc gctgatccgc
     7741 atggccgctc agctcgttca gcttcgccac ctcggccctc aggcgcatcg gctctccgaa
     7801 aatcggaacc tcttgcattt tgtcaacgat cttccgcccg agcgtatttt ccgcctggaa
     7861 cccggtgatc agcaccgtgt tgcgcgggtc ctcgatgttg tttctcagat gatgcagaat
     7921 gcggccggcc tcgcacatcc ccgacgccga gatgaccatg aacggcccgc gcagatcgtt
     7981 cagcgtcttc gattcgctcg catcacggac gtaacggagc gtctggaatc caaacgggtc
     8041 ctcgccttcc agcagatatt gcctcgtctc ctcgtcgaag cactctgagt gcctctggaa
     8101 gatcttggtg gcgtccacgg ccagcgggct gtccacgaat accggaatgc gcgggatctt
     8161 ccttgcgttc gtcagctcgt gcaacagcag cacaagctgc tgcgttcttc cgaccgcgaa
     8221 cgacggcacg atgattttcc ccccgcgcgc cgccgtccgg ttgacgatct ccgccagttt
     8281 cgccgatacg gcgccgagtt ccttgtgtag ccggtcgccg taagtcgtct ccatgatcag
     8341 gtaatccaca ttctccagcg gatccggatc tcggacgatc gcctgcttcg gacgtcccac
     8401 gtctcccgag aatgccagcc ggatgcgctt cccgttctcg tggtaatgca gcagtaccgc
     8461 cgtcgagccg agcatgtgtc cggcgttgta ggactcgtag ctgagcaggt cgtccagccg
     8521 cgtcggcgtg tacatcggca cctggcggaa ctgcttcagc gcgcgctcgg cgtcctcgat
     8581 tgtgtacagc ggctccaccg gtccgttcgt gagctccgga tcgagcgcct tccgacggtg
     8641 gtggcgcttg tttaggtacg ccgcatcttt ctcctggagg tacgccgaat cgcgcaacat
     8701 ggcgtcgcac agatccgccg tcggcggcgt ggcgtagatc ggcccttcga agccttgctt
     8761 caccaggctc ggcaggttgc cgctatgatc gatgtgcgca tgggaaagga cgactgcatc
     8821 cacagatttc ggcgagaacg gaaaactccg gttgcggtcg ttcgcatcct tccgccggcc
     8881 ctggtacagg ccacaatcga gcagaaaccg ccttccgttg acctgcacct cgtgcatcga
     8941 tcccgtgacc gtgcgtacgg cgcc
//
LOCUS       DATIFQ010000180         5744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1018452, whole genome shotgun sequence.
ACCESSION   DATIFQ010000180 DATIFQ010000000
VERSION     DATIFQ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018452"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="VLE22_07965"
     CDS             complement(<1..1291)
                     /locus_tag="VLE22_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14380.1"
                     /translation="MTVARVAVFFILAFSAIAAPLSCDFSGYRAQPGLQAEVSAGSLL
                     VTWDGERGDTLRARFGLEASKPVIRELTIRKRGGEWSVLGKDLSPEYSVVTGLRRTGH
                     GLPEELRWWVYWDAPLSIPGTGDQPNLPRRPEEIRSFTAAFQSTSCAVRTEGSRIEVT
                     FPGLSMGVFGGSLRYTVYRGSNLLRQEAIAKTEEPSLAYKYAGGLKGFQTSQLSRVVW
                     RDVGGNEQSYAFGGAANEGPVPLRARNRVAVAEGPAGSIAVFPPPHQFFFARQLEINL
                     GFVWYRKDGDSSFSLGVRHGDSHEGYNPVWVERVFALYNAPPGTWQRMPVYFYLGAGD
                     AEQTRDFVLSYTRGDRYKPLPGCKTMVTHFHTAFAEELMQAKSLDVQAPWIPAIRALG
                     ADIAHIHDFHGDGHPQDAGPLRLKELENYFAACRRHSD"
     gene            1381..2763
                     /locus_tag="VLE22_07970"
     CDS             1381..2763
                     /locus_tag="VLE22_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB14381.1"
                     /translation="MFDVTKAGLTRRKLLQSGAGMSAFASALKLMDGSQGAQPKAAPP
                     VNKNSAPSDLKITDMRACTVAANYDYPIIRIDTNQGVYGLGEVFAAGVRGSALMLKAH
                     LVGKNPLQISSILRGIRNSAGQNFWNTGYGAIDLALHDIAGKVYGVPVWRLLGEKLRD
                     KVIIYCDTTGHKDAQVYAKRVALRKAAGFKFFKMDLYTSLVKGRPGAVNTRGVATEKG
                     LGYLCELLAAGREVIGWETPLAADHFGPLNVDDAIRYSRAFEPYTLAWAEDLIPECWL
                     NWRGFKAIKEATTTPVLTGEQGFGLAECFRNLIDNYAVDLIHPDYVGSGGLLEIKRIA
                     DYAAAHGIPTVIHQPGSPVGQIAMTHLAAVLTNFVACEHHAADMPWWNDLVTGLPKPM
                     IKDGYVEVPDKPGLGVELNDEVVKKHLREPDYFGPTPQFDEMITSGWRRTGPWPHYDE
                     EGKWCNCVTY"
     gene            2777..3739
                     /locus_tag="VLE22_07975"
     CDS             2777..3739
                     /locus_tag="VLE22_07975"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HSB14382.1"
                     /translation="MKTTRREFLVALGVGAAVRLYGVGGKPIRGVFPIMATPFTESKA
                     VDFEDLEHEVDFLDRCGVHGMVWPQLASEYWLLTKEERFRGMELLAKAIRGRRPALIL
                     GVQGPDTEAMLEYARHAEKLEPDGMIAIPPAQAKTLDDFLNYYRALGKVTKRPVFVQT
                     SGGAQGIEPKVESLVELAREFPNVAYVKEEYSPIIDRMIALARQRPPIRGVFSGAHGK
                     GWTYEMRLGCDGTCPGSPLADIYVRIWDLYQGGEKDKALDVFSKLLLVINIEQQIPGT
                     LEYVMKRRGVFKTAVSRRHEVKLTPEAVAEIEYNLAPLKPWMRA"
     gene            3789..5651
                     /locus_tag="VLE22_07980"
     CDS             3789..5651
                     /locus_tag="VLE22_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSB14383.1"
                     /translation="MRVWRRVFVLLAVVAAAGFCQGLEYIREHYTKYEFRIAMRDGKR
                     LFTAVFAPKDTSQQYPILLLRTPYGVGPYGADNYKNSLGPSEKFAREGYIVAYQDVRG
                     KHESEGEFVNVRPHIPNKRGPQDVDESSDTFDTVDWLVKNVPNNNGRVGIWGISYPGF
                     YAAMGAIDAHPALKAASPQAPVTDWFIGDDFRHNGALFLSHAFAFFARFGRPRPEKAT
                     IPETPFEPGTPDGYQFFLEMGPLFNADERHFKGRIAFWQELLGHDTYDEYWKARSMQA
                     HLKNIKPAVLTVGGWFDAEDVWGPLNVYKAIEQSTPRAVNMLVMGPWTHGQWSSGTKD
                     KVGDIEFHTKSEAFFRDNIEFRFFERYLKDKGEGSLPEAYVFETGRNQWRRQDTWPPK
                     AAISKTLYLREAGRLAGDAPRETGAGVFDEYISDPAKPVPVSGRIQFGMPGDYMTHDQ
                     RFASRRTDVLVYQTEPLETDLTLAGPVTASLHVSTTGTDSDFVVKLIDVYPADFPDPD
                     PNPRGVRMGGYEQLVRGEPFRGKFRNSFEKPEPFQPGKPVKVEFVMPDVYHTFRSGHR
                     MMLHVQSSWFPLVDRNPQKFMQIHLAKEADFQKATQRVYRSRDLPSLVKIMTLP"
BASE COUNT         1159 a         1659 c         1902 g         1024 t
ORIGIN      
        1 ggtccgagtg gcggcgacag gcggcgaagt agttttcgag ctccttcagg cgcaatggtc
       61 cggcgtcctg agggtgcccg tcgccgtgga agtcgtggat atgcgcgatg tcggcaccga
      121 gcgcacggat ggccggaatc cacggcgcct gcacgtccag gctcttggcc tgcatgagtt
      181 cctcggcgaa ggcggtgtgg aagtgcgtga ccatcgtctt gcagccgggc aggggcttgt
      241 agcggtcgcc gcgggtgtag gagagcacga aatcccgcgt ctgctccgca tcgcccgcgc
      301 cgaggtagaa ataaacgggc atgcgctgcc atgtgcccgg aggagcgttg tagagggcaa
      361 aaacgcgctc gacccagacg gggttgtaac cctcatggct gtccccgtgc cgaacaccga
      421 gagagaagga cgagtcgccg tccttccggt accagacgaa gccgagattg atctcgagct
      481 gtcgcgcaaa gaagaactgg tgtggaggcg gaaaaacggc gatgctgccg gcgggacctt
      541 ccgcgacggc cacgcggttc cgcgccctga gtggcacggg gccttcgttt gctgcaccgc
      601 cgaaagcgta tgactgctcg ttgccgccta catcgcgcca gaccacacgg gaaagctgcg
      661 aggtctggaa ccccttgaga ccgccggcgt acttgtaagc cagtgatggc tcctccgtct
      721 tcgcgatcgc ctcctgccgg agaagattcg agccgcggta gacggtgtac cgaaggctgc
      781 ctccgaaaac gcccatggag agccccggga acgtgacttc gatccggctc ccctcggtac
      841 ggaccgcaca gcttgtcgac tggaacgccg cagtgaacga tcggatctcc tcgggcctgc
      901 gcggcaggtt cggctggtcg cccgtgccgg ggatgctgag tggcgcatcc cagtagaccc
      961 accagcgaag ctcctccggc aagccgtgac cggtccggcg caggcccgtg acgacggagt
     1021 actccggcga gaggtctttg ccgagaacac tccactcgcc accccttttt cggatggtca
     1081 actcgcggat taccggcttc gaggcctcaa ggccgaagcg ggcgcggagg gtgtcgccac
     1141 gttcaccatc ccaagtgacg agcaacgagc ctgctgagac ttccgcttgc agtccgggct
     1201 gtgcgcggta accgctgaaa tcacaagaca agggtgcggc aattgcggaa aaggcgagta
     1261 tgaagaaaac agcaacccga gcgaccgtca tgggcgaatt gtaacggaaa aacccgtggc
     1321 gatctcgttg cgggcggagg ctgggttgga tatgctgctt gagccaaagg agcaccgatc
     1381 atgttcgatg tcacgaaagc agggctgaca cgaaggaagc tgctgcaaag cggggcgggg
     1441 atgtcagcct tcgcatccgc actgaagctc atggatggtt cgcagggtgc ccagccgaaa
     1501 gcagctccgc ccgtgaacaa gaactcggcg ccgagcgacc tgaagatcac cgacatgcgg
     1561 gcgtgtacgg tcgccgccaa ttacgactat ccgatcatcc gcatcgacac caaccagggg
     1621 gtgtatggct tgggcgaggt gttcgccgcg ggcgtgcgcg gtagcgcgtt aatgcttaag
     1681 gctcaccttg tcggtaagaa tccgcttcag atcagctcga ttctgagggg tattcgcaac
     1741 tcggccggcc agaacttctg gaacaccggc tatggagcaa tcgacctcgc gctccacgac
     1801 atcgcgggca aggtctacgg cgtgccggtc tggaggctgc ttggagagaa gctgcgggac
     1861 aaggttatca tctattgcga cacgaccggc cacaaggatg cgcaggttta cgcgaagcga
     1921 gtcgcactgc gcaaagccgc ggggttcaag tttttcaaga tggaccttta cacaagcctg
     1981 gtgaagggcc gcccgggcgc agtcaacacc aggggcgtcg cgaccgagaa gggactcggc
     2041 tacttgtgcg agcttcttgc cgcgggccga gaagtgattg gatgggagac cccgctcgcg
     2101 gcggaccatt tcgggcccct gaacgttgac gatgcgattc gctacagtcg cgccttcgaa
     2161 ccgtacacgc tggcgtgggc cgaggacctg atcccggagt gctggctgaa ctggcgcggc
     2221 ttcaaggcga tcaaagaagc gaccaccacc ccggtgctta ccggcgaaca gggcttcggg
     2281 cttgccgagt gcttccgcaa cctgatcgac aactacgcag tggatctgat ccacccggat
     2341 tacgtcggat cgggcggact gctcgagatc aagaggattg cagattatgc ggcggcgcac
     2401 ggcattccaa cggtgatcca ccaacctggt tctcccgtcg ggcagattgc gatgacacat
     2461 ctggcggcgg tgctcaccaa tttcgtcgcg tgcgagcacc acgcggctga catgccgtgg
     2521 tggaacgatc tcgtaacggg attgccgaag ccaatgatca aggacggtta cgtggaagtg
     2581 ccagacaagc cgggactggg cgtggaattg aacgatgagg tggtcaagaa acacctccgg
     2641 gagccggatt acttcggacc gacgccacag ttcgacgaaa tgattacgtc cgggtggcgg
     2701 cggacgggcc cgtggccgca ttatgacgag gaagggaagt ggtgcaactg cgtgacgtat
     2761 tgaaggggag gccggcatga agaccacaag gcgtgaattt ctggtggcgc tcggcgtggg
     2821 cgcggccgtt aggctgtatg gcgttggcgg gaagcccatt cggggggtgt ttccgatcat
     2881 ggcgacgcca ttcacggaat cgaaagccgt ggatttcgaa gatctcgagc atgaggtgga
     2941 ctttctcgac cgttgcggtg tccatggcat ggtctggccc cagctcgcca gcgaatattg
     3001 gctgttgacc aaagaggagc ggttccgcgg gatggagttg ctggcaaagg cgatacgcgg
     3061 gcggaggccg gcgctgatcc tcggcgtgca gggaccggac acggaggcga tgctcgagta
     3121 cgcgcgtcac gcggagaaac tcgagccgga cggcatgatc gccatccctc cggcacaggc
     3181 gaagacgctc gacgacttcc tcaactacta ccgggcgctt gggaaagtga cgaaacgccc
     3241 ggtgttcgtg cagacgagcg gcggagcgca agggatcgag ccgaaggtgg agtcgctggt
     3301 cgagctggcg cgggagtttc ccaacgttgc gtacgtgaaa gaggagtact cgccaattat
     3361 cgatcggatg atcgcgctgg caagacagcg cccaccgatc cgtggcgtct tcagcggcgc
     3421 gcacggcaag ggctggactt acgagatgcg cctgggctgc gacggcacgt gcccgggctc
     3481 gccgctggcg gacatctacg tgaggatctg ggacctttat cagggcggcg agaaagacaa
     3541 agcgctcgac gtcttcagca agctgctgct ggtgatcaat atcgagcaac agattccggg
     3601 cacgctggaa tacgtgatga agcggcgcgg tgtattcaag acagcagttt cacgacggca
     3661 tgaggtaaag ctcacgccgg aagctgtagc ggagatcgag tacaacctcg cgccgctcaa
     3721 accctggatg cgggcttagc ggcgcgtcag cgccgacgcg gccggggctt cgctattatg
     3781 tggaatctat gcgcgtatgg agacgtgtgt tcgtactgct ggcggttgtc gccgccgcgg
     3841 ggttctgtca ggggctcgag tatatccgtg aacactacac gaagtacgag ttccggatcg
     3901 cgatgcggga cggaaaacgg ctgtttacgg ctgtattcgc accgaaggat acgtcccagc
     3961 agtatccgat cctgttgctt cgaacgccat acggcgtggg accgtacggc gcggacaact
     4021 acaagaactc tctgggtccg tcggagaagt tcgcccgtga aggctacatc gtggcgtatc
     4081 aggacgtgcg cggcaaacac gagtccgagg gtgagtttgt caacgtgcgg cctcacattc
     4141 cgaacaagcg cggtccacag gacgtggacg agagttccga tacgttcgac acggttgact
     4201 ggctggtcaa gaacgtgccc aacaacaacg ggcgtgtggg aatctggggc atctcgtatc
     4261 ccgggttcta cgccgcgatg ggcgccatcg acgcacaccc cgccctgaag gccgcatcgc
     4321 cgcaggctcc ggtgacggac tggtttattg gcgacgactt ccggcacaat ggcgccctgt
     4381 ttctctctca cgcgttcgcg ttctttgccc gcttcggccg cccgaggccc gagaaagcga
     4441 cgattccgga gactccgttt gagcccggca cacccgacgg ctaccaattc ttccttgaga
     4501 tgggcccgct tttcaacgcc gacgagaggc acttcaaggg ccggatcgca ttctggcagg
     4561 aactccttgg ccacgacacg tacgacgagt actggaaggc gcggagcatg caggctcacc
     4621 tcaagaacat caagccggcg gtgcttaccg tcggagggtg gttcgacgct gaggacgtct
     4681 ggggtcccct caatgtgtac aaagccatcg agcagtccac gccacgcgcc gtcaacatgc
     4741 tggtgatggg tccttggacg cacggacagt ggtcctcggg gacaaaagac aaagtggggg
     4801 acatcgagtt ccacacgaag tcggaagcgt ttttccgcga caacatcgag ttccgcttct
     4861 ttgagcgcta tctgaaggac aagggggagg ggtcgctgcc ggaggcgtac gtcttcgaga
     4921 cgggacgcaa ccagtggcga cggcaggaca catggccccc gaaagcggca atttcgaaga
     4981 cgctctacct ccgggaggcc ggccgtctgg ccggtgatgc gccgcgggaa accggcgcag
     5041 gcgtgttcga cgaatacatc agcgacccgg caaagccggt gccggtgtcc ggccggatcc
     5101 agttcggaat gccgggcgac tatatgacac acgatcagcg tttcgcctcg cgccggaccg
     5161 atgtgctcgt ctaccagacc gagccgctgg aaaccgacct gacacttgcc ggaccggtga
     5221 ccgccagcct tcacgtctca accaccggta ccgattcgga ctttgtcgtg aagctgattg
     5281 acgtctaccc ggcggacttt ccggatccgg atccgaatcc gcggggcgtc cgcatgggcg
     5341 gctatgagca acttgtgcgc ggggaaccgt ttcgcggaaa gttccgcaat agctttgaga
     5401 aacccgagcc gttccagccg ggcaagccgg tgaaggtgga attcgtgatg ccggacgtct
     5461 accacacctt ccgcagcggc caccggatga tgctgcacgt gcaaagctcg tggttcccgc
     5521 tggtggaccg caatccgcag aagttcatgc agatccatct ggcgaaagag gcggacttcc
     5581 agaaggcgac ccagcgggtc taccggtcgc gggacttgcc gtcgcttgtt aaaatcatga
     5641 cgcttccgta ggtactgaca gctcggtggc gttatgatga ccccaaggag gggaccttgc
     5701 gacgtcatgc actgttgatc ctggctgcgc ttgccgggtt gagc
//
LOCUS       DATIFQ010000181         2524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2358927, whole genome shotgun sequence.
ACCESSION   DATIFQ010000181 DATIFQ010000000
VERSION     DATIFQ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358927"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1168
                     /locus_tag="VLE22_07985"
     CDS             239..1168
                     /locus_tag="VLE22_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB14384.1"
                     /translation="MSAQSSSAIRRSTTAAEPDELVPSRYSLRVGEIDVMVVSDGVLL
                     LPGAMLGHNADPAVRAAWLNDMFLPPDVFEWALNAVVVRSGSRTVLIDAGIGVDPGLN
                     LPRAGRLVQRLEAAGIDLASVTDVVLTHMHMDHIGGLLVDGVKDQLRPDLRIHLAAAE
                     AEFWASPDFSRVSMPPGFPDALRRTAKRFLNEYRSQMRPFEKEYEVAPGVVVQRTGGH
                     TPGHSVVRLASGGDRLTFAGDAVFQVGFDHPDWYNGFEHDPEEAARVRVRLLRELAAN
                     RESLVATHLSFPSVCHVAVHGDVFRWVPGPWEY"
     gene            1340..2188
                     /locus_tag="VLE22_07990"
     CDS             1340..2188
                     /locus_tag="VLE22_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB14385.1"
                     /translation="MRTITTKDGVEIFYKDWGKGQPIVFSHGWPLSADDWDAQMIFFL
                     KHGYRVIAHDRRGHGRSAQVSDGHDMDHYADDLAALTAHLDLKNAIHVGHSTGGGEVA
                     HYLGRHGESRVAKAVLIDAVPPIMVKTPGNPGGLPIEVFDGLRKALAGNRAQFYLDFA
                     SGPFYGFNRPGAKPMPGVIQNWWRQGMMGGINAHYDCIKAFSETDFTDDLKKITVPVL
                     VMHGDDDQIVPYADSGPLSARLLKNGTLKTYKGFPHGCPTTQAETINNDLLAFLRSGV
                     AAKTAA"
     gene            complement(2333..>2524)
                     /locus_tag="VLE22_07995"
     CDS             complement(2333..>2524)
                     /locus_tag="VLE22_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14386.1"
                     /translation="QWFMADEYTIADVSMLGWVRNLIGFYGARELVAFDSLKHVPAWL
                     DRGLARPAVQRGLQIPRRD"
BASE COUNT          493 a          750 c          771 g          510 t
ORIGIN      
        1 cccgcgccga cgaagtgatc gagtagcaac cgatgtccgc tattggccct tagccgacat
       61 tcccgtttgt aaagcggaca tgactatttg cactgcacat ttccgcttat gacccaaagc
      121 ggacattttg acgtgggacc aacgtccaac tcgattaccg ccggttttag gttcatcgtc
      181 acgggcgaaa gcattcacct acatcgaata gcatttccct cgatcgagga gaatgattat
      241 gtccgcacaa tcttcatcag ccattcgccg cagcaccacc gcagcagaac ccgacgagtt
      301 ggttccgtcg cgctactcgt tgcgggtcgg cgagattgac gtaatggtgg tgagcgacgg
      361 ggttctgttg ctgccaggcg cgatgttggg ccataacgcc gacccggccg ttcgggccgc
      421 ctggctgaac gacatgttcc tgcctccgga cgtgttcgag tgggccctca acgcggtcgt
      481 ggtgcgtagc ggaagccgga ccgtactcat cgacgctggg ataggggtgg acccgggctt
      541 aaacttgccc cgggctgggc ggttggtcca gcgactggag gccgccggca tcgatctcgc
      601 atccgtgacc gacgtggtgc ttacccacat gcacatggac cacattggcg ggctgctcgt
      661 cgatggggtg aaggaccagc tgcgtccgga cctgcggatc cacctggcgg ccgccgaggc
      721 tgagttctgg gcgtcgcccg atttctcccg cgtctccatg ccgccggggt ttccggacgc
      781 gcttcggcgg acggccaaac ggttcttgaa cgagtaccgg agtcagatgc ggcctttcga
      841 gaaagagtac gaggtggcgc cgggggtggt cgtccaacgc accggtggcc acactcctgg
      901 gcacagcgta gtccgcctgg cgtccggtgg cgaccggctg acgttcgccg gcgacgccgt
      961 gttccaggtc gggttcgatc acccggactg gtacaacggc ttcgaacacg accccgagga
     1021 ggcggctcga gtccgggtcc gtcttctgcg ggagttggcg gcgaaccgcg agtcgctcgt
     1081 ggccactcac ctgtcgttcc cgtccgtgtg tcatgtggcg gtccacggcg acgtctttcg
     1141 ttgggtaccg ggcccctggg agtattgacc gcttgtgggg ttagggccga accagacgac
     1201 ctgaactatg tccgctattg gcccatagcg aaaacgagct agtgcaccgc acatgtccgc
     1261 tttcgagggg taaagcagac aggactacca aacttgtgca tgtcggcgtc tgacgccgat
     1321 ttcccccgag gagataccca tgcgtacgat taccaccaaa gacggcgtcg agatctttta
     1381 taaggactgg ggcaaagggc agcccatcgt gttcagccat ggctggccgc tatcggccga
     1441 tgactgggac gcgcagatga tttttttcct caagcacggc tatcgcgtca tagctcatga
     1501 ccgccgaggt catggccggt ccgcccaggt aagcgacggt cacgacatgg atcactatgc
     1561 cgatgatctg gcagcgctga cagcgcatct cgacctgaag aacgcaattc acgttggcca
     1621 ttccactggc ggcggcgagg tcgcccacta tctggggcgg cacggcgaaa gccgggtagc
     1681 gaaggcggtc ctaatcgatg cggtgccgcc gatcatggtg aagacgccgg gcaatccggg
     1741 cggcctgccg atcgaggtgt tcgacggctt gcgcaaagcg ctcgctggca accgggcgca
     1801 attctatctc gattttgcga gcggtccttt ctatggattc aaccggccgg gtgcgaaacc
     1861 aatgcccggc gttattcaga actggtggcg tcagggcatg atgggcggca tcaacgcgca
     1921 ctacgactgc atcaaggcgt tctcagagac cgacttcact gacgacctta agaagatcac
     1981 ggtgcccgtc cttgtgatgc atggcgatga cgaccagatc gttccttacg ccgactccgg
     2041 accattgtcg gccaggttac taaagaacgg gacgctaaag acctacaaag gcttcccgca
     2101 cggctgccca accacgcagg ctgagacgat aaataatgac ttgcttgcct tcctgcgttc
     2161 cggggttgcg gccaagaccg cagcgtaact gtgactgaaa gccgagggga gaaatagacc
     2221 gctcctctct cctttgctca gttggtttgg cccttagccg acattcctaa ttgcaccgca
     2281 catgtccgct ttcggatgta gagcggatat tggatattgg gcaggacccg ctctaatctc
     2341 gcctgggtat ctgaagccca cgctgcacgg ctggtctagc aagtccacga tcaagccacg
     2401 caggaacatg ctttaatgaa tcgaatgcga cgagctcgcg tgcgccgtag aagccgatga
     2461 gattgcgtac ccacccgagc atcgaaacat cggcgatcgt gtattcgtcg gccatgaacc
     2521 actg
//
LOCUS       DATIFQ010000182        54293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_636045, whole genome shotgun sequence.
ACCESSION   DATIFQ010000182 DATIFQ010000000
VERSION     DATIFQ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 54293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54293
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636045"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VLE22_08000"
     CDS             complement(<1..356)
                     /locus_tag="VLE22_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14387.1"
                     /translation="MPKIVVDRGQRFQVWRRPSFSVACEGGRRQKAIDFGEHAEQISH
                     RVKEASLATMMAAGRPVQYLNSGKDDKQQLARSIAAADGISCGPICALTAVELCSSYA
                     VRGNRSERKIELQRSYR"
     gene            complement(702..1781)
                     /locus_tag="VLE22_08005"
     CDS             complement(702..1781)
                     /locus_tag="VLE22_08005"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HSB14388.1"
                     /translation="MEHFIGCDAHKRYSVFSCIDERGRYGSDVRVRHQAAEFRAFLES
                     LPPGSEIAIEAGGDYYWMADAMEQAGHHPHLAHPLEAKRRCGKTVKHTDKVDARGLAM
                     LLRNGTLPEVWIPPAELRDERALLRLRMYLSHMRTGLKNRVHGILRQYNLLLAGSDIF
                     GAAGREELAARLAELPPQTRRSVEEQLRLLDFLSARIEQLERRLAGILANSAETELLK
                     TLPKVGPILSAVMALELGDIERFPGAPHVASYCGLVPRVHSSGGRTRLGATCGDVNRT
                     LKWAFVEAANLIVMRQRRLAGTHAVRLYQRLQSKHGHQKAVVAVARHLAEAAYWVLHK
                     QEPYREPKSAPKRLCRRDRVSAACA"
     gene            complement(1923..3887)
                     /locus_tag="VLE22_08010"
     CDS             complement(1923..3887)
                     /locus_tag="VLE22_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HSB14389.1"
                     /translation="MSRKLWIAVAVLGTVASSFAQEGRSRIDVEHYVINAEINPNTQS
                     LAGTASVRFTTQEDRISSVVFELHNALNVSKVEDESGRELGASRSRNDFTVRVAFPEA
                     LEKGKTVTLTFRYDGVLTGKEESPVYGVRFASIQSDHSYLLYPARWFPVSGYTEDRYT
                     AEIKVTVPAGNKVIAGGSEQVAQVTGGKLAYTFTTRQPAFPGSIAVVQGDPLRIASEG
                     VTSDLYFRDAAREMAGPYGQEADKVMTYLTSVFGLPPQANLTFVQTENGAPNGYATTG
                     VIFLSPRSIGDKVNSRLLANQIARQWWGMLLSAESRNHLWITNGAARYAELLYVAQAN
                     GPAAMENDVRETYIEALTVDSPPLIQVSRVEDYSPEFWALTAGKGAAVYHMLRSVIGD
                     EKFFQLLKQLPEKYAWQSVNTGDLRKAAEELTATNLQYFFIQWIESTGAPEFKSEYTI
                     FRTQKGFRVMGKVTQDLDTFRMPVDLKIETEGNPEEKRIEVVGTSSEFIVETFGKPKS
                     VILDPNNRVLRFSNPIRVAVAIRRGEQFVEVGEFGDALKEYSKALEVDRNNSLAQYRV
                     GEVFFKQSNYQSAANAFREALNGSLDPAWVEVWSHLNLGMIFDITGQRERAVNEYKLA
                     VRTKDNTQGAQEIAAKYINEPYQRKGADDR"
     gene            complement(3877..5040)
                     /gene="lpxB"
                     /locus_tag="VLE22_08015"
     CDS             complement(3877..5040)
                     /gene="lpxB"
                     /locus_tag="VLE22_08015"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HSB14390.1"
                     /translation="MSPKILVSAGEPSGDLYAAGLVERLRRRLPDASFFGCAGHHMEQ
                     AGVRPVVHAASLAVVGLLEVVGHIPRIYREYRRLIDAARRERPDLAILTDSPDFNLRV
                     ARRLKTLGIPVVYLVAPQVWAWRKGRISSIRRNIDRLLCIFPFEESYFRHHGVPAEYI
                     GHPLSRMVKPSVSREDFLRRHRLDGENPLIVLLPGSRRGEITRHLPTVLDAVRGIMRR
                     NRTAQFVLALPAGLLRHSGGGWLGSLLGDAPVTPAEGETWDAIAHADLALAASGTVTI
                     ETALLGTPMVTYYRVTGFSWLMGKFLVDVPFYSMVNLVAGRAVVPELMQNEMRGDRLA
                     AEAIRLIENPAARDVMRRDLRDVAALLSTGGDPLERAAAAVQQVLNHGDTNES"
     gene            5181..6137
                     /locus_tag="VLE22_08020"
     CDS             5181..6137
                     /locus_tag="VLE22_08020"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB14391.1"
                     /translation="MSLFHSHPRPKKSSRRKFVFLGMFAPIARVSARKPEPGPGAGPL
                     APPQEVARKPVRKRARVFDAHLHCPSENGQVWQWHTVTKTFEEFVAYLDRTGVERGII
                     NSVRCQLAKSPQEFIAGNREVARYVERYKGRFLGACVVNPMFIDEALREMENCRKQYG
                     MVWVGELCNYTVPYEYTVKEFELLVEQAVKLNMVLDVHTELEEMEFIIRKFPKATIVF
                     PHFGDGNEYGHIFKRIDLVAANPNCYLDTSGYGHDRVGMLEYAVKSIGPDRVLFGSDF
                     SINCPATVIARVEHAFLTEEQRRKILGGNLEALLAKAGWKEA"
     gene            6208..6978
                     /locus_tag="VLE22_08025"
     CDS             6208..6978
                     /locus_tag="VLE22_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14392.1"
                     /translation="MSIETRLDKLSVEEFVDRCREAMTPLGSKFSYFPAIPLTEEEVQ
                     EYLNEPIAALPPALIAALPNLALVLVPYLEKPNGKVQNGTTGEHVSFEKPGEGRQIYV
                     AEASAGDGTLAKLVLAIKDIAISEYHYHFYRALAGILGRGMGENLEEQYVALLREELS
                     NRVHGEVDHAGWHLKQALIRRQTNVRRETKVFREYALQSFVDTMTLYLHGICCDVDVE
                     TGPRQLPSRFLRKRLELLEILFPPPEGYAVFPEEIRGE"
     gene            complement(6987..9326)
                     /locus_tag="VLE22_08030"
     CDS             complement(6987..9326)
                     /locus_tag="VLE22_08030"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSB14393.1"
                     /translation="MNLTLGFPEFGPETGLRRQTGIESAVARFEQWMTDPESPVRAVR
                     RQPARSGEFEVVPETVHASLREVLARRGIERLYTHQAEAFRRVSSGANVVVVTPTASG
                     KTLCYNLPVFNRLIEDPEARAMYLFPTKALAEDQLHEFQETVEAMGSGIRAFTYDGDT
                     PQDARRAIRERANVVLTNPDMLHAGILPHHTRWAKVFENLRYFVIDELHYCRGVYGSH
                     VANVLRRLKRICEFYGSSPQFICCSATIANPRELAESLTGLPFELVDCNGSPAGEKYF
                     VFYNPPVVNRQLGIRRGYLNETRRIALEFIERNQQTLVFANNRLATEVLVTYLKDAAE
                     RSPLGAGAVRGYRGGYLPRERREIERKLRNAEIRAVVATNALELGVDIGTLDTVVMAG
                     YPGSIASTWQRAGRAGRRQGTSLAVLVASSAPVDQYVIEHPDYFFDRSPEHAHINPDN
                     LEILINHLKCAAFELPVRDGERFGPHDAADLCRFLEEAGFLHHSADAWHWTSDTYPAD
                     AISLRAVTSDNFVVVDMTGEPTVIADVDFPAALTALHEKAIYLHEARQYQVERFDYEA
                     RKAYVRSVDCDYFTDAIDYTQVKVLEEFDSAPVSGARAVHGDVRVNTQVVGFKKVKFY
                     TMENVGAGSLSMPEQEVHTTAFWLHFPEGFLSRFPDLTPTERQSGLTGLGNALRAVAS
                     LLVMCDPRDLGVAITEDISGGLKTWEPNLYLYDNYPGGIGQSAPLQKMHRRLLTGAAE
                     LLAACRCESGCPGCVGPIGEVGERGKEVAARILAELLAG"
     gene            complement(9404..9727)
                     /locus_tag="VLE22_08035"
     CDS             complement(9404..9727)
                     /locus_tag="VLE22_08035"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB14394.1"
                     /translation="MIEQRKTKRFELKLPLEVVRKSFQPVSSAGMTKNLSAGGVLFAA
                     SIDVEIGDPIEYVITFPTHSINGGSVNLRCLGKVVRVETPTHSEESGEAVVAATLERY
                     EFVRD"
     gene            9889..10083
                     /locus_tag="VLE22_08040"
     CDS             9889..10083
                     /locus_tag="VLE22_08040"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HSB14395.1"
                     /translation="MRKVLRPILGVSLVLVGIVGLILPVMPGWIFVIPGLVILGDYFP
                     PVRRLLDWAKAKARIGHTSR"
     gene            complement(10080..10931)
                     /locus_tag="VLE22_08045"
     CDS             complement(10080..10931)
                     /locus_tag="VLE22_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14396.1"
                     /translation="MSRTIFVPLVTALSWTVWPAMAAQPDPQAIVRRSIEVDEESGSE
                     ILRNFIFVERSDKKDLDGDGHVKSRVLKTHEVVMHDGTPIRRLIAQDDKPLSAEEQRS
                     QEEGFRKAIEARKRESATERQRRIRESEERRTRYRRAIREIPDAFSFRLAGAERVGGR
                     PAWVLDAEPRPGYRPKDRYSKLFTQLKGRLWIDQDDYHWARAEAELLDSVWFGWILVR
                     IARGAKVKMDQMRLNDGLWVTKHLWYTASARVGLIMTYHVEEEFDYRDYRKVDLESLL
                     AGNVSSH"
     gene            11006..11785
                     /locus_tag="VLE22_08050"
     CDS             11006..11785
                     /locus_tag="VLE22_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14397.1"
                     /translation="MPETLSQSPSVNVLLMAATGIAVLSAAIGVWLIGVPRKGTTLVP
                     FSAGLLLGIVAFGVWPELAEPFGWLGGLALLAGGFALLWSVNRFVYPVCPTCSHTHDH
                     DSCAISLHGFALPLIAAAAVHSVLDGWGIAASRDEGTGSLGLAVFLGVALHKVPEGLA
                     YGAILRAALRSRAGAFAGVLATQLPTMSGELLNRWLGPYLGLHWMGLPLALVGGSFLF
                     LGFHAVHSDLRRRGPAPALVPALTGAVGAAALHHGLRLFLR"
     gene            complement(11792..13222)
                     /locus_tag="VLE22_08055"
     CDS             complement(11792..13222)
                     /locus_tag="VLE22_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSB14398.1"
                     /translation="MANIPDDVFFATIAELNGKLKAKEFSAVELALAFSERLEKSGPR
                     YNALALSLREHALRQAREVDDDIKRGRFRGVLQGIPYGAKDLLSLAGRPTTWGARPYA
                     GQVFDFDATVVKKLGKAGAVLAGKLAMVELAGGGGYRYAAASLTGPGLNPWDTTRWSG
                     GSSSGSGSAVAAGLVPFAIGSETWGSILTPCAYCGVTGLRPTYGLVSRYGAMALSWTM
                     DKIGPICRSAEDCGHVLRVISGGDSNDPGSAGKSFFYAPQYARKLSDLRVGFAPVDFA
                     EWAEPTARPAFQAALEVVRGLGVKTVEIALPDLPYSLAAGTIISAEGAAVFEPLIKSG
                     QVDQLADQHQIAGLKAGLDIAAKDYLKAMRIRSLIREAMRKIFIDVDVLVSPSVFGPA
                     SKISDPLNRGFRPKPEPKDRGLRDLGAVGNLAGLPALSLPCGFTSEKLPVSIQLVGRS
                     FSENNLLALGVEFQKRTDWHRRRPPV"
     gene            complement(13228..13431)
                     /locus_tag="VLE22_08060"
     CDS             complement(13228..13431)
                     /locus_tag="VLE22_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14399.1"
                     /translation="MVRATMTRRELAAALASSTSLVRAQEERKLTPEEVLAQEKADVR
                     SESEQLEKFNVPMATEPAFIFRP"
     gene            13492..14805
                     /locus_tag="VLE22_08065"
     CDS             13492..14805
                     /locus_tag="VLE22_08065"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HSB14400.1"
                     /translation="MLIPNELVVRCLGQRTVDSPLKLSTMRGDGIGDFVPDDAKMLFR
                     PDFSPRDPIRADLSFEKAGPRQQLYFDPASTTAAIVTCGGLCPGLNNVIRSVFFQLHH
                     NYGVRNVVGIRYGYQGLNPVHGVPPVRLTPEFVESIHKLGGTVLGSSRGSEEPRVMVD
                     FLERERIDILFCVGGDGTQRGSHAIAEEVTRRGLPVAIVGIPKTIDNDLNYVWRSFGY
                     YSALDKAREILDGAHAEAKGVPNGIALVKLMGRHAGFIAAGATLASQEVNFTLIPEIP
                     FELEGEHGFLHCLKRRILNRGHAVIVVAEGAGQDLVWQGEAGTDASGNVKLQDIGPFL
                     RDKILDYFARERISAGMKYFDPSYVIRSVPANTDDALLCDRLARNAVHAAMAGKTDVL
                     MGLWYNTFIHVPISLAIRETKRLSPESEVWMSVLASTGQPAQFCR"
     gene            complement(14821..16422)
                     /locus_tag="VLE22_08070"
     CDS             complement(14821..16422)
                     /locus_tag="VLE22_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB14401.1"
                     /translation="MSERRRIPTRRDFLRSAAGAATLASPAILPARAGSRSRPNVLFI
                     ICDDLNDSVDGMGGHPQARTPNIHRLMSRGVQFTNAHNNQPWCAPSRASLWSGIYPFK
                     SHYHGSGNWRQNPVLGQSVTLIEHMRKSGYAVYGTGKLFHNGQEPKDLYTEYGHDPEF
                     GPWPWDGKERREHPTLNCLFETAYYKNLPLLHETPPMQTWGPLSDVPEFMPDPAKGIP
                     GYRGWRQYNASFRYVNEEDRDLIADELCAKWAADVLSRKHETPFFLGVGFSRPHIPLY
                     APKKYFDMFPAENIQLPPYLSNDTDDCARALLLADAKGLEKFRLLHAAGGEMMWKRWI
                     QAYLACVSFADDQVGKVLNALERSPYAQDTVVILTSDHGFHMGEKDYIFKNSLWEEST
                     RVPLVIDAPGMSRSGARCDQPVSLIDMYPTIADLCGLPAEPNARGNGYRLDGFSLRPF
                     LENPRDGYWSGPAFALTELGAGQKAHFSIRGRQWRYTLCANGEEELYDHAADPNEWTN
                     LAGDAKFDKVKAELKRELLRMTAQS"
     gene            complement(16450..17697)
                     /locus_tag="VLE22_08075"
     CDS             complement(16450..17697)
                     /locus_tag="VLE22_08075"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HSB14402.1"
                     /translation="MIRTVLLTLTAAGVLSAQPAYDLLLKGGHVIDPKNRISKVMDVA
                     IAGGKIAKVAPDIPASEAKRVADVRGLYVTPGLIDIHVHVYTGTGLRALTGDSSVQPD
                     AFSFRTGVTTMVDAGTSGYRNFPDFRQRVIDRVRTRVLALLNIAAGGMGPEPGGEDDP
                     KNMDVEATVKMAREHKDVVVGFKSAHYKGPAWFSVERAVAAGKATDMPVMVDFGYTTK
                     ERNLTALLTEKLRPGDIYTHCYSGHRDELLGGKVNPAMWAGRKRGIIFDLGHGGGSFY
                     WYVAVPAFREGFDPDVISTDLHSGSMNAGMKDMINVMSKVLNLGSSIENVVLWSTWKA
                     AQAIKRPELGHLDVGAGADVTVLRLDKGRYGFVDAAGARMSGDKLLVAEMTIRDGEIM
                     WDLNGRASEDWKVFKYKKREAPK"
     gene            complement(17772..19283)
                     /gene="aroE"
                     /locus_tag="VLE22_08080"
     CDS             complement(17772..19283)
                     /gene="aroE"
                     /locus_tag="VLE22_08080"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HSB14403.1"
                     /translation="MPPLKSLPQICIALGFADAGTLAEHARRELDEGETFFEFRLDYL
                     PDPAEGVRAIAGFLEEHPECTILATCRRHQNHGRFNGSIEEQIHILEAAVSAGARAVD
                     VEIESAEGVNAKLEPLRTGANLIISYHNFEGTPPLETVLRRMLKVPADAYKVVTTARK
                     PSDNLRVLVLAKSHPRARLILLAMGEAGFPTRVLSPVFGGLFTYAAPCTAEGTAVGQV
                     SAKQMRHLYRIERLSKGGRIYGVIADPIRHSISPAVHNRAFQSRRVDAVYLPFLVPPV
                     HLRDFVTMAEKLPVAGFSVTIPHKQKIIRYLDVVDPLARRIGAVNTVWRKAGKWRGAN
                     ADAEGVIVPLSRRLRLNRSSVLVVGNGGAARSAVFALTDAGAKVSVVGRNPDRVRALA
                     KAGGAEAVTREQLETLRFDALVHATPLGMFPHVDECFFEDLIPADLVFDMVYNPLDTL
                     LIRRARQQGKEVIPGLEMFIEQAVRQFEIWTGESAPRAAMEKAARETLGANHQ"
     gene            complement(19339..20070)
                     /gene="deoC"
                     /locus_tag="VLE22_08085"
     CDS             complement(19339..20070)
                     /gene="deoC"
                     /locus_tag="VLE22_08085"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HSB14404.1"
                     /translation="MNTESPQVRPPISTYEALAGMIDHSLLRPELTEEEVEQGCRLAR
                     EYRVATVTVRPADVDLAVQWMRDSGVPVGTVAGFPHGSAATAAKLYETRDLLRRGARE
                     IDMVINIGKLLSRRFQYVETELSQMAAACHESGALLKVIFENAYLTDELKIIACKIST
                     RAGADFVKTSTGFAPSGATLEDLRLMRAHSGPSVKVKAAGGVRTLDKAMDVYAAGADR
                     FGATATASILDAWRDKLRQAAQPGQ"
     gene            complement(20101..20967)
                     /locus_tag="VLE22_08090"
     CDS             complement(20101..20967)
                     /locus_tag="VLE22_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14405.1"
                     /translation="MKTCVLTLLLFLWPFLAFAQRKEYVELQRDIAILQDQVRTMQRT
                     LDEKLAQMTLLVQQSLDSINKANTSLAVLENNVRDRLREQEKSVSQPVAGINSKVDQL
                     STQMLALKESIDSLNVRFGKLEQQMVDVNTAVRTLQAPPVPPGEGGGPGGASAEQLYQ
                     GALRDKNGGNLELALKGFTDYVAQFGGTTLAPNAQYYIGEILYLQGQHAEAVRAFDLV
                     LEKYQENDKTPDAHYMKGMSLIKMNQRVKARDEFYTLIKRYPESELSAKAKAQLKAMG
                     LPVSPAKSKRTR"
     gene            complement(21035..21808)
                     /locus_tag="VLE22_08095"
     CDS             complement(21035..21808)
                     /locus_tag="VLE22_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB14406.1"
                     /translation="MSKRNISILLLGFSLILLVAGCKKKVAPPPPPPPPKEEAPPPPA
                     RPTISQFVAEPSTIERGQSATLRWATSNATDVTISQGIGSVPATGDRRVFPNDTTTYT
                     LTAKGPGGTETQSATVRVTVPPPPPPPPPPPPKKSLSERLSTEVQDAFFDYDKFDIRE
                     DARATLTRNAEALRDIFRDFPEAKITLEGHCDERGSAEYNLGLGDRRATAGKEFLVQM
                     GVPADKLNTISYGKERPQCTESNEDCWQRNRRVHFVAGQ"
     gene            complement(21848..23236)
                     /locus_tag="VLE22_08100"
     CDS             complement(21848..23236)
                     /locus_tag="VLE22_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14407.1"
                     /translation="MKTRYLTATACALGIALVVAAQQSDILIKITKGERAAIAVPDLR
                     GSGGAQPFMGAFNETLFADLETSGLFRMVPKTMYPIEVPQRPQDFRKPPEPPKPGRGR
                     PPAPPAATGLWLTDWAGPPTNATYLTVGYAGVQGDRLVLFGWLYNVTQQDLNNAQVFG
                     KIYFGTMDETGARKVAHEFASDIMAQFGYKSLLGSKIYFDSDRTGRGQREIWVMDSDG
                     SNQKRLTTYKSLSLMPAVSPDGTKIAFTSYSRGNPVILLHSLVTGGRLPFYNQTASMN
                     ATPDFTPDGGRIVFSSTAAGSYAQLYICNADGSGLKRLTYTNTIDIEPKVNPKTGNEI
                     VFVSGRSGIPQLYKMNLDGADVQRLTSGEGDAVNPSWHPDGQHVAFSWTRGFAPGNYN
                     IFVMDVATRQFVQLTHGAGRNENPVWAPDGRHLAFSSNREGGNQIWTMLADGTQLRKL
                     TTQGSNLRPVWK"
     gene            complement(23233..24018)
                     /locus_tag="VLE22_08105"
     CDS             complement(23233..24018)
                     /locus_tag="VLE22_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HSB14408.1"
                     /translation="MKAHVDILEGQESLRRPLGVSTILHAVLISGLALQGLIPRRAPE
                     SWGHPDSLGGSAVGITPVSQIPLPSRGGVPNPLASDTESSVPEPPAAKVETVKAPEPD
                     PATVAIKGRTPKRPARATQSRRSGLTPETGPEQVYSSTGRGLSSPLVGRTGSGGVGIG
                     TGGAFGNRFGYYRDLLEQKVAQKWRTDDVDPRLQTAPPAIVTFVIYRNGSTTDVRLEQ
                     SSGNRALDYSALRAIYEASPFPPLPAGYERNDARIEFWFQLRR"
     gene            complement(24055..24507)
                     /locus_tag="VLE22_08110"
     CDS             complement(24055..24507)
                     /locus_tag="VLE22_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSB14409.1"
                     /translation="MAFSANSSNGGQYIGRRRFRGAGSLSEINIVPLVDVVLVLLIIF
                     MLTAHVMEFGLEVEVPKVRQVKDSADELPVVSITVDGRLYLNEQPVNINELAATVRKR
                     FGEAQAVYLRADKGTVWDPIAQVLSALGEAGLKVNAVTQPVDEAERKR"
     gene            complement(24511..25200)
                     /locus_tag="VLE22_08115"
     CDS             complement(24511..25200)
                     /locus_tag="VLE22_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSB14410.1"
                     /translation="MTILSSAAAFWIQVSILDLVMRTSLISKAVLVILLVFSVVSWAV
                     IFSKWNVFRQARTSNVRFLRAFRKANGLEAVALASEQFRQSPLVALFEFGYEEVERQV
                     KARGTLGNKLALERSLQLGTSEELTKLERNMNWLATTASVSPFIGLFGTVLGIIDAFH
                     SIGQAGASSLRAVAPGISDALVATAMGLAAAIPAAVFYNHFGHVIREMGARMDDFSLE
                     FMNLAERNFED"
     gene            25326..27029
                     /gene="recN"
                     /locus_tag="VLE22_08120"
     CDS             25326..27029
                     /gene="recN"
                     /locus_tag="VLE22_08120"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HSB14411.1"
                     /translation="MLVELVVENYAVIERLRVRFHPGLNLLTGETGSGKSIVVDALGL
                     LLGGRAAGDMVRTGAERARISGIFEAGVSRRLRQVLEAAGVEIEDGELLIEREILAGG
                     KSRAFAGSRPVTASLLRELAPHLGDIHGQHEQQRLFDADAQIEMLDAFAGSADLVAEV
                     ADLYLRWRSCRRELDELERNEQEKLRLADLWGFQRREIEEASPKPGEDAELESERRRL
                     LNFAKLEENAASAYEALYESPESAIARMRHAARRLDELARIDPSLGALGESLKSAEFS
                     VEEAAYSLRDYLSHLEGSPGRLEEVETRLAAIEKLKRKYGQTTEQVLAYLEEVRGQLA
                     ALENTESRRAALNEELRSLAAAYEAAAARLSDRRKDAAHKLEKRVETELSSLAMERTA
                     FRVRVGPAAWSEHGVDEVCFLVSPNVGEEPRTLEKVASGGEISRIALALKTCVAAGAG
                     KTTKGEGAPRTLVFDEVDAGIGGRAAEVVGRRLKDLAASSQVLCVTHLPQIAAFADHH
                     YYVEKKEANGRTVATILELTGDSRTREVGRMLSGEKLTPEALKHAEKLIQAGSAGKRG
                     A"
     gene            27077..28126
                     /locus_tag="VLE22_08125"
     CDS             27077..28126
                     /locus_tag="VLE22_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HSB14412.1"
                     /translation="MFANLSIGVAILVICLALAAVFLWPGPGIRRELAKRRELHARAL
                     LEDVLKHILTRRHEGREASVQSIAGQLNLPAQKVYRLIARTESAGLVRSDAGRVQLTA
                     EGERWALQVVRAHRLWETWLANEARMPMAEVHKSAERAEHKFTSKQIDELDAYLGHPR
                     QDPHGDPIPTASGEIASLEARPLTEWPEDTPAQIAHVEDEPVAVFRKITDLGLRPGSV
                     VEIRRRSPDQFTITDGEKEHTLSLLVAGNIQVIQPAQTAARRESLARLSDLVPGEAGE
                     VALLDQELRGFTRRRLLDLGLTAGSRVTAHLTNAFGDPLAYRVRGTTIALRKEQASKI
                     WVRKHANTEKEVRAS"
     gene            28123..28944
                     /locus_tag="VLE22_08130"
     CDS             28123..28944
                     /locus_tag="VLE22_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HSB14413.1"
                     /translation="MNLPGHDCDTCPAHAQLAQMGISLTKYDRLVALAGNPNTGKSSV
                     FNALTGLKQHVGNWPGKTVSRAEGGYVFNGLRYKLVDLPGTYSLLSGSPDEEVSRNFV
                     LFGQPDCTIVVVDATALERNLNLVLQVLEITDKVVVCVNLMDEARRKGLDVDVRSLAR
                     DLGVPAVPTVARTKEGLHELVSTVAGVIQGEIKTSPFRPKHHPELQKAIDELAPLILQ
                     AVPGVPNAPWVAYRLLDGDHRIREALVSGELSELAATQGQPLDRRSSLMSIEGRQ"
     gene            28941..30335
                     /locus_tag="VLE22_08135"
     CDS             28941..30335
                     /locus_tag="VLE22_08135"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HSB14414.1"
                     /translation="MSAGMNVSEGVLARADKLRSALSGDFRDRMVESLYAEADRIANR
                     AVKSGQERKWDLDQRIDRLVTSPIFGIPLMMLLIGTTFWITIIGANYPSQAIATVLFA
                     LEKRGAELFQGLGSPWWLTGFLWNGVYRGLAWVISVMLPPMAIFFPMFTILEDLGYLP
                     RVAFNMDWLFRRAGAHGKQSLTMAMGFGCNAAGVVAARIIDSPRERLIAILTNNFVPC
                     NGRWPTLIMLATIFVAAQFQPAFASFAAAAAVTGVVLIGVVFTLIVSWALSRTILRGE
                     ASAFTLELPPYRRPSVLRILYTSLIDRTLFVLWRAIMTAAPAGAVIWVLANINVGGVS
                     LARHISDFLNPFGHAIGLDGVILLAYIIAIPANEIVVPTMLMVYLGTGMMTEVTSFDD
                     LRRILVGQNGWTLLTAANLMLFSLLHNPCATTILTMWKETRSGKWTAIGALMPLAIAF
                     LVTFAVATVVRLLA"
     gene            complement(30358..31134)
                     /locus_tag="VLE22_08140"
     CDS             complement(30358..31134)
                     /locus_tag="VLE22_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB14415.1"
                     /translation="MIDVNAYLGHFAFRQLRNNTAEGLLRLMDRKRIERAVVSSAASI
                     TYRNAHSGNEEIAAEIRDHRDRLIPFAVLNPAYAGWRDDLKTCHEEFGMKGLRLYPRW
                     HSYKLTDPACLDLVHMAAELGMVVSIPLRAEDRRQRSWLVDVPDVDKDEIAGVVKAAP
                     KAQFILMSGSGFTGSVLGRKNNGLPANYAVEICLLSALLANELGQILENLGEDRVVFG
                     TGMPFHYPDPAILKLDVLEASEAVKEKIRRGNAARLLRLT"
     gene            complement(31134..32009)
                     /locus_tag="VLE22_08145"
     CDS             complement(31134..32009)
                     /locus_tag="VLE22_08145"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB14416.1"
                     /translation="MERTTMKMPRRAFLGSLGAGAALMLRASSAQDTPGSGKVIDAHD
                     HLSNRRQKGWEEADRRMIDAADKLGIDQICCSILPPERPTTLEGFRQCNEWMADATRR
                     FPGRVLGYCFVNPGYTKEAIEEIRRYVEGRGFIGIKLYNDYRITEPVLRPIIELAIEL
                     RVPVLSHAGHTSWLPEPQPRISDGGHLAEAGQAYPEATLICAHIAGGGDWEWTVKALR
                     SAPNVYLDTSGSVIDEGTVEMAVRVLGADRLLFACDMSLTASVGRIRGADISEADRRK
                     ILGGNMQRILNRRRV"
     gene            complement(32033..33229)
                     /locus_tag="VLE22_08150"
     CDS             complement(32033..33229)
                     /locus_tag="VLE22_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSB14417.1"
                     /translation="MLRRTFLRTVPGAVAAQQTTRVTGYEVWSWDRWHEITGENRPQI
                     VSDQTGKADLVDLLTFNGQSINAAGAWSSRRRSILGVIQAILGPSPATRPPLETRTLE
                     ETPSEGYIRRRVMYQSEPGEFVPAFLLVPRKLDAPAPAVICPHQTVQEGKNEPAGLAG
                     NSRLHMALHLVRRGFVTLAYDAACFGERHDPASGHYGDAIAFYKKHPEWSMMGKMAWD
                     LSRGVDYLRSLDFVDPARIGSIGHSHGGYTTLFGMALDDRIAAGASSCGFDTFRHDGN
                     VFRWSHATALMPRLGFYLSSSYIDMRFYSGVPDSETIQTPLDMHYVLAMIAPRPLFLS
                     TSDEDSVFPNSGWSARQALARLEPVYRLLGDEDRIGSYYFRGGHGFPPEAAERAYGWL
                     ERWLRR"
     gene            complement(33290..34864)
                     /locus_tag="VLE22_08155"
     CDS             complement(33290..34864)
                     /locus_tag="VLE22_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB14418.1"
                     /translation="MKILLYNPDNGVTRNFMPHLWMFLLQALTPPGHEVLLIDGNAEP
                     MSDAELVRFVLKNKISLVGIGAMTRMIAKAYRVADAVRAAGVPVVMGGPHVTEIPDEA
                     LGRDGGPRHADAVALGEADETWPRIVEDAARGQLKEVYTPTDEFGQERKPSLQEYPRI
                     PWDTINIDQFNRIPAFAKPVMEHFRTEWETFHIIPIESGRGCPYGCEFCTVTGFFGDS
                     IRFRSNESIVREMLELKARSRRTHGKIAVFFVDDNFAINAKRTKALLRDIIAAGAQLS
                     WVGQISANLLRDDELVDLIADSGGKWIFIGMESIDAANLASVNKTFNKPADYSAVLER
                     LARRNVYAITSFIFGLDNDVPGVAERTLREISQWPPVLPVFGQITPFPATPLYDRLEK
                     EGRLTRPKHWLDFAPFHMAHTPMKMTIPEVQSEVTEAWRNSYSPAATRRALNSIRNEP
                     VPYRISHLIARLFFRGIYFPQKNTWAWLKLVAQNRVAIFGLIRDSFTRWHGAKNDGLP
                     LAFDAPPPSRTDPAAP"
     gene            complement(34981..35331)
                     /locus_tag="VLE22_08160"
     CDS             complement(34981..35331)
                     /locus_tag="VLE22_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSB14419.1"
                     /translation="MTNIFVGNLSYQTTEDELQGAFAAYGTVERVSIVRDRDTGQPRG
                     FAFVEMAGSDDAAKAIQALNGRELNGRAMNVNEARPREERSGGGNRFGGGGGGRGGNR
                     GGGGGFGRRREPRW"
     gene            complement(35491..36678)
                     /locus_tag="VLE22_08165"
     CDS             complement(35491..36678)
                     /locus_tag="VLE22_08165"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14420.1"
                     /translation="MSETRREFLKTAAAATAASHNRILGANDRIRVGGIGTGGRCQYL
                     LSLLEQTGGAEIAAVCDVYEPRRTGARAKLAPNAREFTEYRELLEQKDIDAVFIGSPD
                     HWHVPMTIDAVRAGKDVYVEKPVSHKIAEGPSLIKAVEEAGRIVQVGYQQRSWDLFDM
                     GREIVHSGKLGRVTLVLSHWYQDYIRNFGRLSPVDTAKLDWKRFLGSAPEQPFEQRRF
                     LQWRWYWDFGGGALTDLHSHWGDVVHWFMQSDRPLAAQATGDRYHHTYWECPDTITAS
                     WKYPDYNLVYNGTMVGHLEGGTLVFRGTNAMMRITRDGLAVYPEGVLPAEKTTYPEPV
                     ITARATHDGTVDHVRNFLDSVRSRKKPNADVKSAVAAAQAAHVGNLAYRENRVVRAAD
                     YLA"
     gene            36782..37429
                     /locus_tag="VLE22_08170"
     CDS             36782..37429
                     /locus_tag="VLE22_08170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB14421.1"
                     /translation="MKRPHRTKTLMTAAALVSSALAAYPADPARKSPEFRKEFLERFQ
                     RTGLNTTPGDAMMLRILVESRGAKRGVEVGAASGFGAMNMGIAFERTGGHLYTLEIDP
                     KKAEETRQNLRKVGLETCVSVVEGDALKTLPTLTGEFDFVFLDALKQDYMKYLKLIEP
                     KLKPGAVIVADNVIVSEKAMADFLAYVQTSPNFDTVIIRASSEKGDGMSVSYKIR"
     gene            complement(37461..38057)
                     /locus_tag="VLE22_08175"
     CDS             complement(37461..38057)
                     /locus_tag="VLE22_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14422.1"
                     /translation="MACHILGTPNMALRLTAPVSVECVKQEGKGKYTFPQRSIIRFDF
                     PARGPMPAVKIFWHDGMDAQPKVDGVPAGEMLGDDDVNGSLFIGDKGMVTTGCYGGHT
                     RLVPAERMQDYKLPPPLLTRSPGHHRDWIRACKGREPACSNFSVAGPFAEWMLLGVVA
                     MKFEGKLDWDAGKKRFTNNSEANRYLKPKFRKGWKFAG"
     gene            38393..39646
                     /locus_tag="VLE22_08180"
     CDS             38393..39646
                     /locus_tag="VLE22_08180"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HSB14423.1"
                     /translation="MRRVAPGFLVGLACTLSAFAQGGAIAVKEVATPNLGEAYAGQAA
                     VADNASTAYLNPAGMTRLESSETLIGGQLAALGVRFQPNSFSTMKGSDGGDIREYALL
                     PGTYVVRRLTDRLQFGFSFNAPIGLSLDYSEDWIGRYLSRHISMAILEFHPSVGVRVN
                     RWLSLGFGAAIQRASLTQKQAGNNSLFVSGYPDGLIHASHHDWGFGTQYRALIEPSRR
                     TRIGVTYHSYIKYVMKGTIEMEDVTTPLATTLARNGKSDNRMRMPQAADVSLYHDVTD
                     RFALLTDVGWTAWRHFGEKTYTLPSGAKNTVHRKWRDTFRIGLGMRRKLNDRHMLQAG
                     VSYDSNPVSYWDRTPDLPVDRQIRYATGWQYLWRVGLILGFAVSYADLGAARLDTPYN
                     PLQGKLAGRYELARLPFVSLTMQFK"
     gene            39727..42246
                     /locus_tag="VLE22_08185"
     CDS             39727..42246
                     /locus_tag="VLE22_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HSB14424.1"
                     /translation="MRRRCKPEIMSPAGDWPQLRAAVEAGADAVYFGLKHFSARAKVG
                     FPLDELPDVMRTIHQRGVRGYITFNTLVFEHELAEAARAIVAIAEASADAIIVQDYGV
                     LQLAHQIAPDMVLHASTQMSITNSDGVKLAQSFGVSRVTLARELSLDEVRTIVDQSGC
                     EIEIFVHGALCVAYSGQCFSSEAWGGRSANRGQCAQACRLPYELIVDGRLQPLGDARY
                     LLSPGDLYTLSQIPEIVETGVAALKIEGRYKDADYVALATLAYRKAVDEAWAGRKSRI
                     SREEELQLEQVYSRGMGPFFLVGTNHQEVVRGRAPRHRGVFIGRVIQVESDRVLIEPG
                     NTHRIAPLKPGDGVVFDAADWRSPEDPEEGGRVYEVLPAPDSRLALRFGNGAIDFSRI
                     RPGDLLWRTHDPDLDRVVGPYTGAPAPVSRQPVHVKVIAVEGAPLVAEWSLVDQPGAS
                     VTVKSLASLDRAQNRRLSAESLRGQFGRLGNTPYELAGVALDVEGAPFAPASLLNQLR
                     REAVEQLQSLQSRPRHVTVNDPSAAVSAVLGQRSADAPAEDAPPELHLLVRTPEQLEA
                     AQALNPASITLDYLDLYGLRPSLERIKSGGLEARVASPRVLKPGEGRIVDFLLSLECA
                     ILLRSAGMLHALRAREHPPLIGDFSLNAANVLAARTYLELGLSRLTPTHDLNAEQVAD
                     LARSAGAERIEAVVYQHLPVFHTEHCVFCRFLSNGTSYRDCGRPCEKHRVALKDQAGR
                     AHPVMADVGCRNTVFGAEAQEASRHLEAWRRAGIRYFRLEFVHESAGAVRRIVRAFQR
                     ALAGATPPTQLAAELKSIVPEGTTEGSLFVPDGYLFLPVLR"
     gene            complement(42369..45866)
                     /locus_tag="VLE22_08190"
     CDS             complement(42369..45866)
                     /locus_tag="VLE22_08190"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB14425.1"
                     /translation="MRACFLAVLSLLFLSAAEMSAQSLFATLTGVVTDQSQAVAPNAK
                     VKLVNEASGSTRETVTNDEGFFTFTSVAVGNFTYTLTVEMQGFVPYKATGLALQGGER
                     RNVNVTLTVGTTTEAIEVTGIAESIVPVDSGEKSQTLTTKELENFVVLGSNAAQFIRI
                     MPGFGIQNGVRNKSNYDGQTIGINANGDAGSQSPLNNAYSYNGLPSNSLDITADGAHV
                     SDPGCNCDTPVNPNADMVSEFKVLTSNFSAENQKGPAIVSTVTKAGGKEFHGSAFFSA
                     RHFSLNANDWLFNKSGQKAPENKYFFPGGTIGGPVLIPGTNFNKNRDKLFFFTGYEYF
                     YQTLDTGLVRATVPTPDMLQGNFTPEELAKLGTKTALGVAPQQLNEASLLKWPGARIP
                     ASQLDPNMVALAKLYPAANADPNATGGYNFVKALNFNQNNIQWMSRVDYNISDNTKLF
                     VRYNLQRETQRFPVGLWWRNTDQVPYPTPILGKNRSDSISASLTHVFSPSMTNEFVFG
                     YTFIGFPNVFDDPSKVDRAAVGYNYKGLYKNGVAQIPSFGDYGPSEAAFMFNPGGFEA
                     GGPSSGLYANKYMPSVSDTVAKVFGTHTVKAGFFWEWIRNAQPANGNSNGLLSVRATA
                     NPNTTGSTYADLLLGVLNSYNEQSFNRINDIAYNTYEGFVQDSWKASRQLTVEFGVRL
                     THFQPWIDREGFGFSIFDYSKYSPTCKPSDYCGFLWHARDPSVPLGGFPTRTVFWQPR
                     LGFAYDLFGKGQTVLRGGWGRFYFHAGQFTNGLDVAAGVQTINLSNTVDGEPLTAARL
                     DTLSFTSQALSPAAVDGTSDKQPYTDSFSFTVAQRTPWSGLLELAYVGNRTRDIPSSG
                     NGGSLGFNTMNINLVPVGAMLASNNGGVDPNTLTANDFRPLKGFSDLNLATFNGYSNY
                     DSLQVTWVRAKGRYNVNLNYTFGKAMGLINFDDQFNLRNNYGVLPSDRRHLFNVAYSF
                     ELGNPVKSKVAGGVLNGWQLSGITQVQSGANLWPNSSNRNFGMNLNNAKVLGTDHNIS
                     NVSILGTPNLQLRPLLTCDPRSGLGKNQYINASCFAMPTSIGTNGPTVLPPIYGPAFF
                     NWDLGLFKNFSISESRKLQFRFDGYNFLNHPLWSFPSSNNLNLIFNPATGKVDNPNFG
                     IVTEKEGRRLIQMTVKFLF"
     gene            complement(46095..47765)
                     /locus_tag="VLE22_08195"
     CDS             complement(46095..47765)
                     /locus_tag="VLE22_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB14426.1"
                     /translation="MIRMAVLAALVLCACLWGVLLSGRPPKAAGRPHFVDVASRSKIS
                     YKTNNSSEGRKWFPQPMCGGVAIFDFDNDGRMDIFFTNGAHLPELKKTDPSFYNCLLR
                     QRADGTFEDVTARAGLTGDHLDYNFGVAAGDYDNDGYEDLFIASAARNVLYHNNGNGT
                     FTDVTEASGIGGKPANTLSVVAAWFDYDNDGLLDLIVSDYTFWSPKNDFRCTMDSKDY
                     YCDPRRYPSVPPRLYRNLDGGKFADVTKKSGLAAAPGKGMGISIADFNNDGWPDIFIA
                     NDTEPNSLFINQKNGTFEEKGLELGVAYNESAHTGSSMGSDAKDFDNDGNVDIFYNNL
                     MTQIWQLLRNRGDLFVFYSFPSKIQALSMPYSGWSNGFIDYNNDGWKDIYSANGDVDE
                     VNENSPQHDTMFENVGGRTFVDVSEEMGGDFLRKGYQRGSAFGDLNNDGSVDIVVTSL
                     NQSPRILRNTADSGNRWLVLDLVGHKSARDAIGAKVKLTTASGRRLHNHVAVSVGFMS
                     SSDKRVHFGLGQETKISSLEIRWPSGMQQVLEDIPANQFLKIDEPRAK"
     gene            47838..49496
                     /locus_tag="VLE22_08200"
     CDS             47838..49496
                     /locus_tag="VLE22_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB14427.1"
                     /translation="MKVLVSIVVGAFGLLAGSVVSVVFEDIAGRSGLVFVSNSSPTPN
                     KNQPETMVAGVALLDYDNDGYLDVYFVNGATIPSLKKETPQFWNRLYHNNRDGTFTNV
                     TEKAGMKGDGYGMGAATGDYDNDGWTDIYVVNVTRNELFRNNRDGTFTDVTDKASVSG
                     GILDGKKMWSVSAAWLDYNNDGLLDLFVSNYCKWEVNKDPFCGPKPSLRAYCHPQNYG
                     ALPNTLYRNNGDGTFSDVSSETGIGEHPGKGMGAAIADYDGDGFMDIFVANDTYPNFL
                     FHNLKGKKFEEVALEGGVAYSQAGHPLSGMGADFRDVDNDGRADIWHTSVENESFPLY
                     QNRGGGQFVDKTIPGLLAGTRHMSGWSNGVFDFDNDGWKDLFVARGNVLDNIAQFSDR
                     TYGEQNTVFRNLANGKFEDASSNAGTDLQKAAPHRGAAFGDIDNDGRVDAIVTVLNDK
                     AKYFHNISRNSNHWILLKFTGRKSNRMALGAQIRITADDGLKQYNEVTTAVGYACSSD
                     SRVHFGLGASKSIREIEIRWPSGIRQTLTNVAADQILEIQETAN"
     gene            complement(49506..50627)
                     /locus_tag="VLE22_08205"
     CDS             complement(49506..50627)
                     /locus_tag="VLE22_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14428.1"
                     /translation="MPRRLSFLPALSVFWLFAFAQETPERILQQAIAAHRAGNMETAI
                     QQYRAYLKLRPDARDARSNLGAALASTGRYTDAITEYQQALKLGPKDPRIWLNLALAF
                     YKAGEISEAARELSSLHAADPANQQVTLLLADCWLRQGENTRVVDLLTPLEKQNQNDL
                     AIAYLLGTALLRDKQIERGQQMIDRILRNGDSAEAHLLLGTAKLEALDYPAAIADFQK
                     AVKLKPKLPDVYSYLGRAQMLSGNMPAARDAFEKELERNPNDYESNLRLAVLLKENGE
                     FDRAQGLLARALRVRAGDLRALYQVGATDLAAGKLEKACATLEEVVKQAPQFLEAHVS
                     LAQVYYRLKRKTDGDRERALVQKLRAEQEASQARGKIEK"
     gene            50826..51101
                     /locus_tag="VLE22_08210"
                     /pseudo
     CDS             50826..51101
                     /locus_tag="VLE22_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
     gene            51168..51308
                     /locus_tag="VLE22_08215"
     CDS             51168..51308
                     /locus_tag="VLE22_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14429.1"
                     /translation="MREHVTDSPLGQPLDAYEWLLFISAHSERHTKQILEVKADPNFP
                     KN"
     gene            51324..51809
                     /locus_tag="VLE22_08220"
     CDS             51324..51809
                     /locus_tag="VLE22_08220"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HSB14430.1"
                     /translation="MQTEPQKEHQWLQKLVGEWTYESDVAMKPGEPPAKCEGTESVRS
                     LGPLWILAEGEGEIPGCGHATTMMTLGYDPEKKRYVGTWVGSMMSHLWVYDGALDSAG
                     LALALDTEGPSMAGDGKMTKYQDVIQFKSNDNRLLISQVLGDDGKWHQFMSTTYRRKK
                     L"
     gene            complement(52006..52479)
                     /locus_tag="VLE22_08225"
     CDS             complement(52006..52479)
                     /locus_tag="VLE22_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14431.1"
                     /translation="METKPTHEQAQLHLQVYDLRREARLRQARDWFFKNYIVDTFEEA
                     MRIAAPGTESGAFAGMVMSYWEQACALLNYGLLHEDLFFETSGEFFGVWELLKSIVPQ
                     ARERFADKQFLGHMEKAAQRFESWSERRSPGHVAAMRQFMQQMRTQPKAAQKASA"
     gene            complement(52704..53027)
                     /locus_tag="VLE22_08230"
     CDS             complement(52704..53027)
                     /locus_tag="VLE22_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB14432.1"
                     /translation="MKQAEATHRSTVLTALEETENALVGYSNEQERCERLQRVIRADR
                     TVLELAQAQYRAGLAEFLTVLDAERALSEHEDQLAQSQTAVVTRLISLYKALGGGWSV
                     PPSTP"
     gene            53004..53414
                     /locus_tag="VLE22_08235"
     CDS             53004..53414
                     /locus_tag="VLE22_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HSB14433.1"
                     /translation="MGGLRLLHARFLVARAIHALSGRRQGTFVKLNCAAIPTGLLESE
                     TFGHEKGAFTGAIAQRVGRFELAHKGTVFLDEVGEIPLELQTKLLRVLQEREFERLGS
                     SRTMRTDARVIGATNRTREDMVRDHKFVPTFTTG"
     gene            53507..53878
                     /locus_tag="VLE22_08240"
     CDS             53507..53878
                     /locus_tag="VLE22_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB14434.1"
                     /translation="MNKSVATIPAEDMRALVSYPWPGNIRELQNLIERAVILSSGPVL
                     AIPVQQLEKAAALSASAAASAPAPDVITLEDAEREAILRALRNSGGRVSGQHGAATAL
                     GTKRTTLQARMRKLGINPRGI"
     gene            complement(54094..>54293)
                     /locus_tag="VLE22_08245"
     CDS             complement(54094..>54293)
                     /locus_tag="VLE22_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14435.1"
                     /translation="ASIVGTRYLTYVSAGMAGEIRADYVGTDSLMQRLLIWIGVAWRY
                     HAVHIPIWRKLRRQKKQEQHT"
BASE COUNT        10187 a        16710 c        16735 g        10661 t
ORIGIN      
        1 cgataggacc gctggagttc gatcttccgc tcactgcggt tgccgcgcac cgcatagctg
       61 gaacacagtt ccacggcggt cagggcgcag atcggtccgc aggaaatgcc gtcggctgcc
      121 gcgatggacc gtgccaactg ctgcttgtca tccttgccgg agttcaagta ctgaaccggt
      181 cggcccgccg ccatcatggt ggccagggaa gcctctttga cacggtggct gatctgctcg
      241 gcatgttcgc caaagtcgat cgccttttgt cgtctgccgc cctcgcaggc cacggaaaac
      301 gatggcctgc gccatacctg aaagcgctgc cccctgtcaa cgacgatttt aggcaaacat
      361 caaacgttca ttgcctcgga ctcgcgccgc tcgagcaacg gctgcccttc cctcttgtaa
      421 gaagggggtg gcaacgcggg caggacccgt caaggccgcg cagcgccgca gggaagcctt
      481 gaggggtgcc cgcgttgcca cattcaagtc tgcccgaggg aagggcgggc tttgctcacc
      541 cggcccctcc cgtcatgtca aagatctcgt ctgcctccgc cgctcgccgc tttggattcc
      601 tatgccggct gctggtgcgc cggagccata tcttcgctct ccatctccgc atgaatccgt
      661 tccgcgggcg ggtgtcgcaa tcacaaaaca gcctctcagg gctaggcgca ggccgcgctt
      721 acccggtcgc gtcggcacaa tcgtttcggg gcagatttcg gctctcgata gggttcctgc
      781 ttgtgtaaaa cccagtaggc ggcttcggcc agatgccgcg ccaccgccac caccgctttc
      841 tgatgtccgt gcttggactg gaggcgctga tagagccgca ccgcgtgcgt cccggccagc
      901 ctgcgctgac gcatgacgat cagattggca gcttcgacga aggcccattt gagcgtgcgg
      961 ttcacgtccc cgcaggtcgc acccagccgc gttcgcccgc cgctggaatg cacgcgcggc
     1021 accaggccgc aataactggc cacgtgcggg gccccgggaa accgctcgat gtcgcccagt
     1081 tccagcgcca tcacagcgct caagatcggt cccaccttgg gcagggtctt cagcagctcc
     1141 gtctccgcgc tgttggccag gatccccgcc aggcggcgct ccagctgctc gatacgcgcg
     1201 ctcaggaaat ccagcagccg cagctgctct tccacgctgc gccgcgtctg cggcggcaac
     1261 tccgccagcc gagcggcgag ttcctcccgg cccgcggcgc cgaagatatc gctgcctgcc
     1321 agcagcagat tgtactgccg caagatgccg tgcacccggt tcttcagccc ggtccgcatg
     1381 tggctcagat acatgcgcag ccgtagcagc gcgcgctcgt cccgcagctc ggccggcgga
     1441 atccagactt ccggcagggt gccgttgcgc aacagcatgg ccagcccgcg cgcatccact
     1501 ttgtcggtgt gcttgacggt cttgccacag cgccgcttgg cttccagcgg atgcgccaga
     1561 tggggatggt gtccggcctg ctccatcgca tccgccatcc agtagtaatc gccgcccgcc
     1621 tcgatcgcga tctcgctgcc cggcggcaag ctctccagaa aggcgcggaa ttcagcggct
     1681 tggtgacgta cgcgaacgtc gctcccgtag cggccgcgct cgtcgataca gctgaacacc
     1741 gaatacctct tgtgggcgtc acatccaata aaatgttcca tggatactgc agctctcctt
     1801 tgagctgatg cgcggcccac cggacccgaa cggcaccagc cgttcgggcc tagcgggccg
     1861 ctcttttggt tctgccatat cctggcagaa cgcgctgcag cgctttcatg gaatcacacc
     1921 agctaccggt cgtcggcccc tttgcgctgg tacggctcgt tgatgtactt cgccgcgatt
     1981 tcctgcgcgc cctgggtgtt gtccttcgtg cgaacagcca gtttgtactc attgacggcg
     2041 cgttcgcgct ggccggtgat gtcgaaaatc atcccgaggt tgaggtggga ccagacctcg
     2101 acccaagccg gatcgaggct gccgttcagc gcttcccgga acgcgttggc cgcggactga
     2161 tagttgctct gcttgaagaa cacctcgccg acgcggtact gcgcgaggga gttgttccgg
     2221 tcgacttcca gcgccttgga atactccttc agcgcatctc cgaactcacc cacttcgacg
     2281 aactgctcac cgcggcgtat cgccaccgca acccggatcg ggttactgaa gcgcaacacg
     2341 cggttgttgg ggtcgaggat aaccgatttc ggtttaccga acgtctcgac gatgaactcg
     2401 gaggaggtgc cgacgacctc gatgcgcttc tcctcgggat tgccctcggt ctcgatcttg
     2461 aggtcgaccg gcatgcggaa ggtgtcgagg tcctgggtta ccttgcccat gacccgaaag
     2521 cctttttgcg tacggaagat cgtgtattcg ctcttgaact cgggagcgcc ggtggactcg
     2581 atccactgaa tgaagaagta ctgcaggttc gtggcggtaa gttcctcggc cgccttgcgc
     2641 agatcgcccg tgttcaccga ctgccacgcg tatttctccg gaagctgctt cagcaactgg
     2701 aagaacttct cgtcaccgat caccgagcgg agcatgtgat agacagccgc tcctttaccc
     2761 gcggtcagcg cccagaactc cggagagtag tcttcgacgc gggaaacctg gatcagcggc
     2821 gggctgtcca cggtgagcgc ctcgatgtac gtctcgcgga catcgttctc catggccgcg
     2881 gggccgttgg cctgtgcgac atagagcaac tccgcgtacc gcgccgcccc gttggtgatc
     2941 cagaggtgat tacgcgactc ggcggagagg agcatgcccc accactggcg ggcgatctgg
     3001 ttggcgagca gccgggagtt caccttgtcg ccgatcgagc gcggcgagag gaagatgacg
     3061 ccagtggtag cgtaaccgtt cggggcgccg ttctcggtct gcacgaacgt aaggttcgcc
     3121 tggggcggca ggccgaacac gctggtcagg taggtcatga ccttgtcggc ttcctggcca
     3181 tacgggccgg ccatctcgcg tgcggcgtcg cgaaagtaga gatcagaggt gaccccctcg
     3241 gaagcgattc gcagcggatc tccctgcacc accgcgatgc tgccggggaa cgcaggctgg
     3301 cgcgtggtga acgtgtatgc aagtttaccg cccgtgacct gcgcgacctg ttccgaaccg
     3361 ccggcgatca ctttattgcc cgccggcacg gtcactttga tttccgcagt gtaacggtct
     3421 tcggtgtagc cgctgactgg aaaccaacga gccggataga gcaggtagga gtgatcactt
     3481 tggatcgacg cgaagcggac accatagacc ggcgattctt ctttgccggt caggacgccg
     3541 tcgtagcgga acgtgagagt gactgtcttg cctttctcca gcgcctcggg aaaggcgacg
     3601 cgcaccgtga agtcgttgcg ggaccgtgaa gcccccagtt cgcggccgga ctcgtcctcc
     3661 acttttgaga cgtttagtgc attgtgaagt tcaaaaacga cggacgagat ccgatcttcc
     3721 tgagtggtga aacggaccga tgccgtgccg gcgagcgact gggtgttcgg attgatctcg
     3781 gcgttgatga cgtagtgctc gacatcgatg cgcgagcggc cttcttgtgc gaacgaagaa
     3841 gcaaccgtgc caagaaccgc caccgcgatc cacaacttac gactcatttg tatctccgtg
     3901 gttaagtacc tgctgtaccg cggcggctgc ccgctcgagc ggatccccgc cggtcgacag
     3961 gagcgccgcg acatctctca ggtcccgcct catcacatct cgtgcggccg gattttcaat
     4021 cagccggatg gcctccgccg ccagacggtc ccctctcatt tcattctgca tcagttccgg
     4081 aacaaccgct cgtcccgcca ccaggttcac catcgaatag aacggcacat ccaccagaaa
     4141 cttgcccatc agccaactga atccggtcac ccgatagtag gtgaccatgg gagtgccgag
     4201 cagcgctgtc tcgatggtca cggtaccact ggcagccagc gccaggtccg cgtgagcgat
     4261 agcgtcccat gtttctccct ccgctggagt caccggagcg tcgccaagaa gcgaaccgag
     4321 ccacccgcca ccactgtgac gaagcaaacc cgctggcagc gccagcacga actgcgcggt
     4381 acgattcctc cgcatgatgc cgcgaactgc atcgaggacc gttgggaggt gacgagtgat
     4441 ctcgccccgc cggctacccg ggagcaacac gataagcgga ttctcgccgt caagacgatg
     4501 cctccggagg aaatcctccc gggaaacgga aggcttcacc atccgggaaa gcgggtgacc
     4561 gatatactcc gccggaaccc cgtggtgcct aaaatacgac tcctcaaaag gaaagatgca
     4621 taacagacgg tctatgttgc gccgaattga ggaaattctt cccttccgcc acgcccaaac
     4681 ctggggcgcc accagataga cgacgggaat tccaagggtt ttcagccgcc gggcaacacg
     4741 aaggttgaag tcggggctgt cggtcaggat ggccagatcg ggtctctcgc ggcgcgcggc
     4801 atcgatcaga cggcggtatt cccggtagat ccgcgggata tgcccgacta cctccagcag
     4861 ccccacgaca gccagactgg ccgcatgaac caccggccgc acgccggctt gttccatgtg
     4921 gtgacccgcg cacccgaaga aggaggcgtc cgggaggcgg cggcggaggc gctcgacaag
     4981 accggcagca tagagatcac cggaaggctc accggcggaa accaggatct tgggggacac
     5041 caagggctag tctagcaagg aggcttgagg aagcaacgcg gccggaccgt ggggcaaacg
     5101 ccggaccggc ggtacgagta gcggcaagca ggacttccgg gagcctcctg gggtagtcta
     5161 gctgtggtca ggaggattcc atgagcctgt ttcacagcca ccctcggccg aagaagtctt
     5221 cgcgccgaaa attcgttttc cttggaatgt tcgctccgat cgcgcgagtt tccgcgcgta
     5281 agccggagcc ggggcccggg gccgggccgt tggcgccgcc gcaggaagtg gcccggaaac
     5341 ccgtccggaa gcgggcgcgg gtgttcgacg cccacttaca ctgcccgagc gagaacggcc
     5401 aagtctggca gtggcacacg gtgaccaaga cgttcgagga gttcgtcgcc tacctcgacc
     5461 gcaccggagt cgagcgcggc atcatcaact ccgtccggtg tcagttggcc aagagcccgc
     5521 aggagttcat cgccggaaac cgggaggtcg cgcggtacgt ggagcgctac aagggcaggt
     5581 tcctgggagc gtgcgtggtg aaccccatgt tcatcgacga agcgctgcgc gagatggaga
     5641 actgccggaa acagtacggc atggtgtggg tgggagagct gtgcaactac acggtgcctt
     5701 acgaatacac ggtgaaggag ttcgagctcc ttgtggaaca ggcggtgaaa ctgaacatgg
     5761 tgctcgacgt ccataccgaa cttgaggaga tggaattcat catccgcaag ttcccgaagg
     5821 cgacgatcgt gtttccccat ttcggcgacg gcaacgagta cggtcacatc ttcaagcgga
     5881 tcgacctggt ggcggcgaat cccaactgct acctcgacac atccggctac ggacacgacc
     5941 gggtgggcat gctcgaatac gcggtgaaga gcatcggccc cgatcgggtg ctgttcgggt
     6001 cggacttttc gatcaactgt cctgctacgg tcatcgcgcg cgtcgaacac gcgtttctga
     6061 ccgaagaaca gcggcgaaaa atcctcggcg gcaatctcga agcgctgctc gcgaaagcgg
     6121 gctggaagga agcctgaccg gtgtacggaa cccgcccaag acagccgtcc gacagacact
     6181 gttttgttac gatgaagtaa agcctaaatg tccatagaaa cccgcctgga caagctgagt
     6241 gtcgaagaat tcgtggatcg ctgccgggag gcgatgaccc cgctgggcag caagttctcg
     6301 tatttccccg cgattccgtt gacggaagaa gaagttcagg agtatctcaa cgaaccgatt
     6361 gcggcgctgc cgccggcgct gatcgccgca ttgcccaacc tggcgctggt gctggtgccg
     6421 tacctggaaa agccgaacgg aaaggtacag aacggaacga ccggcgagca cgtaagcttc
     6481 gagaagcccg gtgagggacg ccagatctac gtggctgagg cgtccgctgg ggacgggacg
     6541 ttggccaagc tggttcttgc gatcaaggac attgcgattt ccgagtacca ctaccacttc
     6601 taccgggcgc tcgccggcat cctgggtcgg ggcatggggg aaaacctaga ggagcagtat
     6661 gtggccttac tccgtgagga actcagcaac cgggttcacg gagaggtgga tcatgccggt
     6721 tggcatctca agcaagccct cataaggcgc cagacgaacg tccgccggga gaccaaggtg
     6781 ttccgcgagt acgctctaca gtcgtttgtc gatacgatga cactctacct gcacggaatc
     6841 tgttgcgacg ttgacgtcga aacaggtccg aggcagttgc cgagccggtt cctgcgaaag
     6901 cggcttgaac tgctcgaaat cctgtttccg cctcccgagg gatacgccgt ttttcccgag
     6961 gaaatccggg gcgagtagcg ccgccgtcat cccgcaagca attcagccag aatacgggcg
     7021 gccacttctt tgccgcgctc gccgacctcg ccgatcggcc ctacgcaacc cgggcagccg
     7081 gactcgcacc ggcaggcggc caggagctca gcagcgccgg taagcagacg ccggtgcatt
     7141 ttttggagcg gcgcgctttg cccaataccg ccggggtagt tgtcgtagag gtacaggttc
     7201 ggctcccagg tcttcaaacc gcctgagatg tcttcggtga tggccacgcc caggtcccgg
     7261 ggatcgcaca tcacgagtaa tgaagcgacc gcccgaagcg cgttgcccaa cccggtgagt
     7321 ccgctttgcc gttccgtcgg cgtcaggtcc ggaaaacgtg acagaaaacc ctccgggaaa
     7381 tgaagccaga atgcggtggt gtgcacttcc tgttccggca tcgaaaggct tccggcgccg
     7441 acgttctcca tcgtgtagaa cttcaccttc ttaaagccga ctacttgcgt gttcacgcga
     7501 acatcgccgt gaacggcgcg ggcgccggat accggagccg agtcgaactc ctccagaacc
     7561 ttcacctgcg tgtagtcgat ggcgtcggta aagtagtcac agtcgacgct gcgaacatag
     7621 gccttgcggg cttcgtagtc gaagcgttcc acctggtact ggcgggcctc gtgcagatag
     7681 atcgcttttt cgtgcagtgc ggtgagcgcg gcggggaaat cgacatcggc gatcacggtt
     7741 ggctcaccgg tcatgtccac aacgacgaag ttgtcgctcg tgacggcacg aaggctgatg
     7801 gcatcggccg ggtaagtatc ggacgtccag tgccaggcgt cggccgagtg gtggagaaag
     7861 ccggcctctt caaggaaccg gcagaggtcc gcggcatcgt gcggaccgaa cctctcgccg
     7921 tcgcgcacgg gcagctcaaa agcggcgcat ttcaggtgat tgatcaggat ttccaggttg
     7981 tccgggttga tgtgggcgtg ctccggagac cggtcgaaga agtagtcggg atgctcgatg
     8041 acgtactggt cgaccggcgc actagaggcc accagcaccg ccagtgacgt gccctgccgc
     8101 cgtcccgctc ggcccgcgcg ctgccacgtc gacgcgatgg aacccgggta gccggccatg
     8161 acgacggtgt cgagcgtgcc gatatcgacc cccaactcaa gggcgttagt ggcgacgacc
     8221 gcgcgaatct cggcgttacg aagcttgcgt tcaatctcgc ggcgttctcg cggaagatag
     8281 cctccccgat atccgcggac ggcgccggcg cccagggggc tgcgttcggc ggcgtccttc
     8341 aggtaggtga ccaggacctc ggtggcaagc cggttgttgg caaagacgag cgtctgctga
     8401 tttcgttcga tgaactcgag cgcaatgcgc cgtgtttcgt tcaggtaacc gcggcggatg
     8461 ccgagctgcc ggttcacgac gggcggattg tagaagacga agtacttctc gccggcgggc
     8521 gagccattgc agtcgacgag ctcaaagggg aggccggtca ggctctcggc cagttcccgt
     8581 ggattggcga tagtagcgga acagcagatg aattgcggcg aagagccata aaactcgcag
     8641 atccgcttca gacgccggag gacgttcgcc acatggcttc cgtagacgcc acggcagtag
     8701 tggagctcat cgatcacgaa atagcggagg ttttcgaaca cttttgccca acgcgtgtgg
     8761 tgcggcagaa ttcccgcgtg gagcatgtcg gggttggtga gcaccacatt ggcgcgctcg
     8821 cggatcgccc ggcgtgcgtc ctgtggggtg tcgccatcgt aggtaaaggc gcggataccc
     8881 gagcccatcg cctccaccgt ttcctggaat tcgtgcaact ggtcttcggc gagcgccttg
     8941 gtggggaaga ggtacatcgc gcgggcttcg ggatcctcga tgaggcggtt gaacacgggc
     9001 aggttgtagc agagggtctt gccgctggcg gtcggcgtca caacgaccac gttcgctccg
     9061 gacgagactc ggcggaacgc ctcggcctgg tgtgtgtaca gccgctcgat tccccttcga
     9121 gcgagaactt cgcgaagcga ggcgtggacc gtctccggca ccacttcaaa ttcgccggac
     9181 ctcgctggct gtcgccgaac cgcgcgcaca ggcgattccg ggtccgtcat ccactgctca
     9241 aaacgggcga ctgccgactc gatcccggtc tggcgtctca gccctgtctc cggaccgaat
     9301 tctgggaaac ctagggtcag attcattatc gactttcgcc ttttctttgc tacagtacac
     9361 cactttccga ggcggaacaa cggagagcac cgaagaagca ggcttagtcg cggacgaact
     9421 cgtaccgctc gagggtcgcc gcgacgacag cctccccgct ttcttcggaa tgggtaggcg
     9481 tctcgacgcg cacgaccttg ccgaggcaac gcagattgac gctgccaccg ttgatcgaat
     9541 gggtggggaa ggtaatgacg tactcgatgg gatcgccgat ctcgacatcg attgacgcgg
     9601 cgaaaaggac acctccagcg ctgagattct tggtcatccc agcactcgac accggctgaa
     9661 aactcttccg cacaacttct agcggtagct tcagctcaaa tcgcttcgtc tttcgctgtt
     9721 cgatcacagg ctatcttaat ctagttccaa ttgtattcca ggtcaagtgc ggaacgcata
     9781 gaaacaaaaa gagtagtaat ggtacccaga cctgtaggac ttctagcctc ttcaggactg
     9841 cagtcccggt ttcgctcaac aacaatgcta aaatgtcggt gaaaaccctt gcgtaaggta
     9901 ctccgtccga ttctgggtgt gtcgcttgtc ctggtgggca ttgtcgggtt gattcttccg
     9961 gtgatgccgg gctggatctt tgtgatccct ggactggtga ttctcggcga ctattttccg
    10021 ccggtgcggc gactccttga ctgggccaaa gcaaaagccc gtatagggca taccagccgc
    10081 tagtgtgagc tgacgttgcc ggcaagcaga gactccagat ccaccttgcg gtaatcccgg
    10141 tagtcgaatt cctcttcgac gtggtacgtc atgattaggc cgactcgggc tgatgctgtg
    10201 taccagagat gtttcgttac ccagaggccg tcgttgagcc tcatctggtc catcttgacc
    10261 tttgcgcccc tggcgatccg gaccaaaatc cagccgaacc agacggaatc gagcagctcc
    10321 gcttcagccc gcgcccaatg gtagtcgtcc tgatcaatcc agagtctgcc cttgagctgc
    10381 gtgaacaact tggagtagcg gtccttcggt cgatagccgg gccgcggctc tgcgtccaga
    10441 acccacgcgg gccggccgcc gacgcgctcc gcgccggcca ggcggaaact gaacgcgtct
    10501 ggaatctcac ggatggccct ccgatagcgg gtgcgtcgct cttcggactc gcggattctc
    10561 ctctggcgtt ccgtggcgct ctcccgcttt ctcgcttcga tggccttgcg gaatccctct
    10621 tcctgcgagc gctgctcttc ggctgacagg ggcttgtcgt cctgggcgat cagccggcgg
    10681 atcggcgtcc cgtcgtgcat gaccacttcg tgagtcttga gaacccggga cttgacgtgt
    10741 ccatcgccgt ccaggtcctt tttgtcggag cgctccacga agatgaagtt ccggaggatc
    10801 tcgctgccgg attcctcgtc cacttcgatg gaacggcgca cgatcgcctg cggatcgggc
    10861 tgcgccgcca ttgctggcca gaccgtccac gacagggcgg tgaccagtgg aacaaagatc
    10921 gtcctcgaca tcccctagat ttcagtctag cggcttgcga gcggatttcg cacttggccg
    10981 ggtacgctac ggtagaattg gattcttgcc tgagacactg tcccaaagcc cctctgtcaa
    11041 tgttctcctg atggcggcga ccggcatcgc cgttttgagc gccgccatcg gcgtgtggct
    11101 gatcggcgtg cctcgaaagg gcacaacgct cgtcccgttc agcgccggtc tgctgttggg
    11161 cattgtggcg ttcggcgtat ggcccgaact ggcagagccg ttcggctggc tcggcggtct
    11221 tgctctgctc gccggcggtt tcgcgctgct gtggagcgtg aaccgtttcg tttacccggt
    11281 atgcccgacg tgcagtcata cgcacgacca cgattcctgc gccatctctc tgcatggttt
    11341 cgcgctgccg ctaatcgccg cggcagccgt tcatagcgtc ctggacgggt ggggaatcgc
    11401 ggcgtcacgg gatgaaggca cggggagcct cggcctggcg gtcttcctgg gagtggcgct
    11461 gcacaaagtc ccggaaggat tggcgtatgg agctatcctg cgggcggcgc tgcgctccag
    11521 ggcgggtgcg ttcgctgggg tgctcgccac acagctgccg accatgagcg gcgaactctt
    11581 gaaccgttgg ctcggtccct acctcgggct gcactggatg ggcttgccgc tcgcactggt
    11641 gggcggcagc ttcttgttcc tcggttttca cgccgtgcac agcgacctgc ggaggcgcgg
    11701 gcccgcaccg gcgcttgttc cggcgcttac gggcgcggtt ggggcggccg cgctgcacca
    11761 cgggctgcga ctcttcttgc gatagcggag cttataccgg cggccggcgg cggtgccaat
    11821 ccgtacgctt ctggaactcc acgccgaggg cgagcaagtt gttctcgctg aaggaccgcc
    11881 cgacaagctg gatcgacacc ggcagctttt cggacgtgaa gccacaaggc agagagagcg
    11941 caggaagacc ggccaggttg ccgacagcgc caaggtcacg aagcccacgg tctttcggct
    12001 cgggcttcgg ccggaacccc cgattcagcg gatcgctgat cttcgaggcc ggcccgaaga
    12061 cgcttggcga tacgagcacg tccacgtcga taaagatctt ccgcatggcc tcgcggatga
    12121 ggctgcggat gcgcatggcc ttcaagtagt ccttagcggc gatatccagt cccgctttca
    12181 atccggcgat ctggtgctga tcggcgagct gatccacttg gccgctcttg atgagcggct
    12241 cgaagaccgc cgcaccttcg gcggagatga tcgtgccggc cgccaggctg tatgggaggt
    12301 ccggcagagc gatttcgacc gtcttcacgc cgagtccgcg gacgacttcg agcgccgcct
    12361 ggaacgccgg ccgcgcggtg ggctccgccc actccgcgaa gtccactggg gcgaagccga
    12421 ctcgcaggtc gctgagcttg cgagcgtact gcggcgcata aaagaaactc tttccagccg
    12481 agcctggatc gttagagtca ccgcccgaga tcacgcgcag gacgtgcccg caatcctcgg
    12541 ccgaacggca gatcgggccg atcttgtcca tcgtccagga aagagccatg gccccgtacc
    12601 ggctgacgag gccgtacgtc ggtctcagtc cggtgacgcc gcagtaagca cacggggtca
    12661 ggatggatcc ccacgtctcc gaaccgatgg caaaaggcac aaggcccgcg gcaaccgcac
    12721 tgccagaccc gctggaggac ccgccagacc agcgcgtggt gtcccatgga ttcagtccgg
    12781 ggccggtgag ggatgccgcc gcataccggt accccccccc gcccgcgagt tccaccatcg
    12841 caagcttgcc ggcaagaacc gcaccggcct tgccgagttt cttgaccacc gtcgcgtcga
    12901 aatcgaagac ctgccccgcg taagggcgcg cgccccacgt tgtgggacgg cccgccaggc
    12961 tcagcagatc cttggcgccg tatggaattc cctggagcac tccacgaaaa cggccgcgct
    13021 tgatgtcgtc atcgacttcg cgcgcctggc ggagcgcgtg ttcgcgcagg gacaatgcca
    13081 gcgcgttgta gcggggtccg gacttttcga ggcgctccga aaaggcgagc gccagttcaa
    13141 ccgcggaaaa ctccttcgcc ttcagcttcc cattcagctc cgcgatcgtg gcgaagaaaa
    13201 cgtcgtcggg gatattggcc atggcggtca aggtctgaag atgaaagccg gctccgtggc
    13261 catcggcacg ttgaactttt cgagttgttc ggactcgctg cggacatcgg ccttttcctg
    13321 cgccagcacc tcctccggcg tgagtttccg ctcctcctgt gctctcacga gcgacgtcga
    13381 agaagccagc gcggcggcca gttcgcggcg cgtcatcgtt gctcgtacca tgcggccatt
    13441 atagtcgcgc ccgcgcttgc gccgcccgag tgctgtgcca gagtagaaga gatgctgata
    13501 ccgaacgaac ttgtcgtccg ttgtcttggc cagcgaaccg tcgattcacc cttgaagctc
    13561 tcaacgatgc ggggcgacgg aatcggagat tttgtgcccg acgatgccaa gatgctgttc
    13621 cgtccggatt tttcgccccg cgatccgatc cgcgccgatc tgtctttcga gaaggcgggt
    13681 ccccggcagc aactgtactt cgatcccgcc agtacaacag ccgcgatcgt tacctgcgga
    13741 ggcctgtgcc cgggcctgaa caacgtgatc cgttcggtct tcttccagtt gcatcacaac
    13801 tacggcgtgc gaaacgtggt cggcatccgg tacggctacc agggacttaa cccggtgcac
    13861 ggagttccgc cggttcgtct gacgccggag ttcgtcgagt cgatccacaa gctgggcggc
    13921 accgtgctgg gttcgtcgcg gggatcggaa gagcctcgcg tgatggtgga ctttctggaa
    13981 agggagcgga tcgacatcct cttctgcgtg ggcggtgacg gcacccagcg cggctcccat
    14041 gcgatcgccg aggaggtgac gcggcgtggg ctccccgtcg ccatcgtcgg cattccgaag
    14101 accatcgaca acgacctcaa ctacgtctgg cgctcgttcg gctactacag tgctctcgac
    14161 aaggctcggg agatcctgga tggggcgcac gccgaagcga agggcgttcc gaacggcatc
    14221 gccctggtta agctgatggg gcgccacgca ggtttcatcg ccgccggggc gacgcttgcc
    14281 agccaggagg tcaacttcac gctgattccg gagataccgt tcgagttgga aggcgagcac
    14341 ggcttcctcc actgcctgaa gcggcggatc ctgaaccgtg gtcatgccgt catcgttgtc
    14401 gccgagggag ccgggcagga cctcgtttgg cagggcgaag ccggaacgga cgcatcgggc
    14461 aacgtcaagc ttcaagacat cggaccgttt ctgcgcgaca agatcctaga ctacttcgct
    14521 cgcgagcgca tctccgccgg tatgaagtac ttcgatccga gctacgtgat ccgcagcgtt
    14581 ccggccaata cagacgacgc gctgctgtgc gaccgcctgg cgcgaaacgc ggtccatgcg
    14641 gcgatggcgg gcaagacgga cgtgctcatg gggctgtggt acaacacgtt cattcacgtt
    14701 ccgatctcgc tggcgattcg cgagacaaag cggctgtccc cggagagcga agtctggatg
    14761 tcggtgctgg cgtccaccgg ccagccggcg cagttctgcc gatagcgggc agagcgcggg
    14821 ctacgactgt gcggtcatcc gcaaaagctc gcgcttcaat tcggccttga ccttgtcgaa
    14881 tttcgcatcc ccggcgagat tcgtccactc gttcggatcc gcggcatggt cgtagagttc
    14941 ctcctcgccg ttagcgcaca acgtataacg ccactgccgg ccgcggatgg agaagtgcgc
    15001 cttttggccg gcgcctagct ccgtgagggc gaaggccgga ccactccagt atccgtcccg
    15061 agggttttca aggaaggggc gcaaactgaa tccgtcgagc cggtaaccgt tgccgcgggc
    15121 attcggctcc gccggcaagc cgcacaggtc ggctatcgtc gggtacatgt cgatcagcga
    15181 aactggttgg tcgcagcgcg caccgctcct gctcattccc ggggcgtcga tgaccagcgg
    15241 aacgcgagtc gactcctccc atagggagtt tttgaagatg tagtcctttt cacccatatg
    15301 gaagccgtgg tcgctggtga gaatgacgac ggtgtcctgg gcatacggac tgcgttccag
    15361 cgcgttcaga accttcccca cctgatcgtc ggcgaacgag acacaggcga ggtaggcctg
    15421 aatccaccgt ttccacatca tctcgccacc ggcggcgtga agcaggcgga acttttcgag
    15481 gcccttagcg tcggccagaa gaagggcgcg tgcgcagtcg tccgtatcgt tcgacaggta
    15541 cggcggaagc tggatgttct cggccgggaa catgtcgaag tactttttcg gagcgtacag
    15601 tgggatatgc gggcgagaga agccgacgcc gagaaagaac ggcgtctcgt gcttgcggga
    15661 gagtacgtca gcggcccatt tcgcgcaaag ttcgtcggcg atcaagtcac gatcttcctc
    15721 gttcacgtag cggaaggagg cgttgtactg ccgccagccc cggtaaccgg gaatcccctt
    15781 ggccgggtcg ggcatgaact ccggcacgtc cgagagcggg ccccaggtct gcatgggagg
    15841 cgtctcatgc agcagcggga ggttcttgta gtacgcggtc tcgaaaaggc agttgagggt
    15901 cggatgttcg cgccgctcct ttccgtccca cggccaggga ccgaactccg ggtcgtggcc
    15961 gtattcggta tagaggtctt tcggctcttg cccgttgtgg aacagttttc cggttccgta
    16021 cacggcgtac cccgatttcc tcatgtgctc gatcagggtc acggattgcc ccagaaccgg
    16081 gttctgccgc cagtttccag agccatggta gtggctctta aacggataga tgccgctcca
    16141 gaggctcgct cgcgacgggg cgcaccacgg ctggttattg tgggcgttgg tgaattggac
    16201 gccccggctc atcagacggt ggatgttggg tgtgcgcgcc tgagggtggc cgcccattcc
    16261 gtccacggaa tcgttcaggt cgtcgcagat gatgaacagc acgttgggcc gggaacgcga
    16321 accggcgcgt gctggcagga tggcgggtga ggccagcgtc gcggcgccgg cggcggagcg
    16381 caggaagtct ctgcgcgtgg ggatgcggcg tctttcgctc attttgattc tccggcctct
    16441 cgaagcggtc tacttcggcg cctcacgctt tttgtacttg aaaaccttcc agtcctcgga
    16501 cgcgcggccg ttcaggtccc acatgatctc gccgtcgcgg atggtcattt cggcgactag
    16561 cagcttgtct cccgacatgc gagcaccggc ggcgtcgaca aagccgtacc tgcctttgtc
    16621 gaggcgtagc acggtgacgt cagcgccggc gcccacgtcg agatgtccga gttcaggccg
    16681 tttgatcgct tgggcggcct tccatgtcga ccagaggacg acattctcga tggacgagcc
    16741 gaggttcaaa accttcgaca tgacgttgat catgtccttc atgccggcgt tcatgctgcc
    16801 gctgtggagg tcggtggaga tcacgtccgg gtcgaagcct tcgcggaacg ccggcacggc
    16861 gacgtaccag tagaagctac cgccgccatg gccgagatcg aagatgattc cgcgcttgcg
    16921 ccctgcccac attgcagggt tgaccttgcc gccgagcaac tcatcgcggt gccccgagta
    16981 gcagtgcgtg taaatatctc cggggcgcag cttttccgtt aggagagctg tgaggttgcg
    17041 ctccttcgtc gtgtatccga agtccaccat gacgggcata tccgtggctt tcccggctgc
    17101 aacagcccgc tccaccgaga accaggccgg ccccttgtag tgcgcagact tgaaacccac
    17161 gacgacatcc ttgtgttcac gcgccatctt gaccgtggct tcgacatcca tgtttttcgg
    17221 gtcgtcctca ccgccgggct cgggacccat acctccggcg gcgatgttca gcagcgccag
    17281 cacgcgcgtg cggactcggt cgatgacgcg ctgccggaag tcgggaaagt tgcggtagcc
    17341 ggaggtgccc gcgtccacca tcgtggtgac gccggtccgg aaggagaatg catccggctg
    17401 aacgctgctg tcgccggtca gagcgcgcag gccggtacct gtgtagacat gaacgtggat
    17461 atcgatcaaa cccggtgtga catagaggcc gcgcacgtcc gcgacgcgtt tcgcttcgga
    17521 agcaggaata tccggcgcga ctttggcgat cttgccgccc gcgatggcga catccatgac
    17581 cttactgatt cggttcttcg gatcgattac gtgcccgccc ttgagcagaa ggtcgtaagc
    17641 cggctgggct gagagaacgc ccgccgccgt taaagtgagg agaacggttc gaatcatgat
    17701 tgcacctttc tgaagaatcc tactccggtc ccggaccgcg ctgttcccgg aaggactggc
    17761 tcgatcgacg ttcactggtg gttcgcaccg agcgtttccc gcgcggcctt ctccatcgcc
    17821 gctctcggcg cgctctcgcc agtccagatt tcgaactggc ggacggcctg ttcaatgaac
    17881 atctccaggc ccgggatcac ttctttgccc tgctgccggg cgcgtcgaat gagaagcgta
    17941 tcgagcgggt tatacaccat gtcgaaaacg agatccgcgg ggatgaggtc ttcgaagaag
    18001 cattcgtcga catggggaaa catgccgagc ggcgtggcat gcacgagcgc atcgaaacgt
    18061 aaggtctcca gttgttctcg cgttaccgcc tcggcgccgc ccgccttggc gagggcgcgc
    18121 acgcggtccg gattgcgccc cacaacggaa accttcgcac cggcgtcagt gagtgcgaac
    18181 actgcactgc gagcggcgcc accgttgccc acgaccagca cggacgaccg attcagccgc
    18241 agccgccggg agagcggtac gatcacaccc tcggcgtcgg cgttggcgcc acgccacttt
    18301 cccgctttcc gccacaccgt gttcacggca ccgatccggc gcgccaacgg gtcgacgacg
    18361 tccaggtagc ggatgatctt ctgcttgtgc ggaatcgtca cgctgaaacc cgcaaccggc
    18421 agtttctcgg ccatggtgac aaaatcccgc aggtggaccg gcggcacgag aaacggcaga
    18481 tagacggcgt cgacgcgccg cgattggaag gcccggttat ggaccgctgg cgaaatcgag
    18541 tgccggatcg ggtccgcgat taccccgtag attctgccgc ccttggacag cctttcgatc
    18601 cggtacaggt gtcgcatctg cttcgcgctc acctggccga cggccgtacc ctcggccgta
    18661 caaggcgccg cataggtgaa taatccaccg aacaccggcg aaagcacgcg agtcgggaag
    18721 cccgcctcgc ccatcgccag cagaatcagc cgggcacgag ggtgggattt cgccagcacc
    18781 agaacacgga ggttgtcgga cggcttccgc gcggtcgtga ccaccttgta ggcgtcggcc
    18841 gggactttca gcattcgccg cagcaccgtc tcgagcggtg gcgttccctc aaagttgtga
    18901 taggagatga taaggttcgc gccggttctc aacggctcga gctttgcgtt cacgccctcg
    18961 gcgctctcaa tttccacatc aacggcccgg gcgcccgcac tgacggcggc ttcgaggatg
    19021 tgaatctgtt cttcgatgct gccgttgaaa cggccgtggt tctgatgccg gcggcaggtt
    19081 gccaggatag tgcattccgg gtgttcttcg aggaatccgg caatagcccg gacgccctcg
    19141 gcggggtccg gaaggtaatc caggcgaaat tcgaaaaacg tttcgccttc gtccaactcc
    19201 cgtcttgcgt gctcggcgag ggtcccggca tcggcaaacc caagcgcaat acagatctgc
    19261 ggcagggact ttagaggcgg catctgactt cggcgacgaa ccagaatatc acgaaccgct
    19321 gcgcccgcag cctcgaaatc actgccccgg ctgggctgcc tgccgaagct tgtctcgcca
    19381 ggcatcgaga atcgaggcgg tggccgtggc gccgaaccgg tctgcaccgg cggcgtaaac
    19441 atccatggcc ttgtcgaggg tgcgaacccc gccggccgcc ttgaccttca cggaaggccc
    19501 ggagtgggcc cgcatcagcc gcagatcctc caacgtggcg cctgatgggg caaagcccgt
    19561 cgaggtcttt acgaaatccg caccggcgcg agtcgagatc ttgcaggcga tgatcttgag
    19621 ctcgtcggta aggtaggcgt tttcaaagat gaccttcagt agcgcgccgg attcgtggca
    19681 ggcggcggcc atctggctca gttcagtttc gacgtattgg aagcgtcggg agagcagctt
    19741 accgatattg atcaccatgt cgatctcccg cgcgccgcgc cgcagaagat cgcgtgtctc
    19801 atagagtttt gccgccgtgg cggccgatcc gtgcggaaac ccggcgaccg tgccgaccgg
    19861 caccccgctg tcacgcatcc actgcacggc caggtcgacg tccgccggac ggacggtgac
    19921 ggtcgccacg cggtattcac gagccagccg gcagccctgc tcgacctctt cctcggtgag
    19981 ctcgggccgc aacagcgagt ggtcgatcat cccggccagc gcttcatacg tggaaatcgg
    20041 gggacggacc tgtggacttt cggtattcat cgaaacagtc tccaggaagg cgcgagccgg
    20101 ctagcgggta cgcttcgatt tcgccgggct gaccggcagc cccatcgctt tcagttgtgc
    20161 cttcgccttc gccgacaatt cactttccgg gtagcgcttg atcagagtgt agaactcatc
    20221 gcgcgccttg acgcgctggt tcattttgat cagcgacatg cctttcatgt agtgcgcatc
    20281 cggcgtttta tcgttttcct ggtatttttc gagcaccaga tcgaaagccc tgacagcttc
    20341 cgcatgttgg ccttgaagat agaggatctc accgatgtaa tactgcgcgt tgggagcaag
    20401 cgttgtgccg ccgaactgtg cgacatagtc cgtgaagcct ttgagggcca actcgaggtt
    20461 tccgccgttc ttgtcacgca acgcgccctg gtaaagctgt tcggcagatg cgccgcccgg
    20521 tccgccgccc tcgccaggcg gcaccggagg agcctggagg gtccgcacgg cggtatttac
    20581 gtcaaccatc tgttgctcaa gcttgccaaa ccggacgttg aggctgtcta tagactcctt
    20641 gagcgccagc atctgcgtgg agagttgatc gaccttgctg ttgatgccgg ccactggctg
    20701 ggagacgctc ttctcctgct ctcggagccg gtcccggacg ttattctcca gcacggcgag
    20761 agatgtgttc gccttgttga tgctgtcgag cgactgttga acgagcagcg tcatctgcgc
    20821 cagtttctcg tccagcgtgc gctgcattgt gcggacctgg tcctggagaa ttgcgatgtc
    20881 ccgctgcaat tcgacgtact ccttgcgttg cgcaaaggcc aggaagggcc acaagaacag
    20941 caacagagtc agaacacacg tcttcatcgt gctctcccga ttacaggacg ccacgggctg
    21001 gaagctgcgc ctccagcccg cgggattaac tcagctactg gccggccacg aagtggacgc
    21061 ggcggttcct ctgccagcag tcctcgttgg actcggtgca ctgaggccgc tctttgccgt
    21121 aactgatcgt gttcagcttg tcggccggta ctcccatctg cacgaggaac tccttgccgg
    21181 cggtggcacg gcggtcgccg agaccgaggt tgtactccgc ggagccgcgt tcgtcgcaat
    21241 gaccctcaag agtgatcttc gcctctggaa agtcgcggaa gatgtcccga agagcctcgg
    21301 cgttgcgggt cagcgtggcc ctcgcgtcct cgcgaatatc gaacttgtcg tagtcgaaga
    21361 acgcatcctg gacctccgtc gacagccgct cgctcagact tttcttcggc ggcggcggtg
    21421 gcggcggtgg cggtggcggc ggcacggtta ccctgacggt ggcggactgc gtctcggttc
    21481 cacccggacc cttggcggtc agcgtatacg tcgtcgtgtc gttcgggaac acccggcggt
    21541 cgccggttgc tggcacggag ccgattccct ggctgatcgt cacatcggtt gcattgctgg
    21601 tcgcccagcg caacgtcgca gactgcccgc gctcgatcgt cgacggctcg gcgacaaact
    21661 gagaaatcgt cggtcttgcc ggcgggggcg gcgcttcttc cttgggcggt ggcggtggtg
    21721 gcggtggcgc caccttcttt ttgcagcctg ccacgagcag aatcagggag aaccccaata
    21781 acagaatgct gatgtttcgt ttgctcatcc tgaacttcgc ctcctatgaa tcacgaactc
    21841 tcaaattcta cttccacact ggccgcaagt tgctgccctg cgtggtgagc ttccgcaact
    21901 gcgtgccgtc agcgagcatc gtccaaatct ggttcccacc ctcacgattg gaggagaagg
    21961 ccaaatggcg gccatctggg gcccagaccg ggttttcgtt acgaccggcg ccgtgcgtca
    22021 attgcacgaa ctgacgcgtt gcgacatcca tcacaaagat gttgtaattg cctggcgcaa
    22081 agcctcgcgt ccacgagaat gccacgtgct gtccgtcagg gtgccaggag ggattcactg
    22141 cgtctccttc ccctgaggtc agcctctgga cgtcggctcc gtccaagttc attttataca
    22201 actgcggaat gccggagcgc cccgagacga atacgatctc gttgcccgtc ttcgggttca
    22261 ctttcggctc aatgtcgatt gtgttcgtat acgttagccg cttcaggccg cttccatcgg
    22321 cgttgcagat gtaaagctgc gcataactgc cagccgcggt cgatgaaaac acgatcctcc
    22381 cgccgtccgg cgtgaagtcc ggggtcgcgt tcatcgaggc tgtctggttg tagaacggca
    22441 atcgaccacc tgtcacaagc gaatgcaaca atataacagg gttgcccctg ctatagctag
    22501 taaaggcaat cttcgttccg tccggagaaa cagccggcat cagcgaaagc gacttgtacg
    22561 tggtcaaccg cttctggttc gatccgtccg aatccatcac ccagatctca cgctggcctc
    22621 ttccggtacg gtctgaatca aagtagattt ttgaacccag caggctcttg tagccgaact
    22681 gggccatgat gtccgaagcg aattcgtggg cgactttgcg cgcgcccgtc tcgtccatcg
    22741 tgccgaaata aatcttgccg aacacctggg cgttgttgag gtcctgctgc gtgacgttgt
    22801 agagccagcc gaagagtaca agacggtcgc cctgcacgcc cgcgtacccc accgtgaggt
    22861 atgtcgcgtt ggtgggcggc cctgcccaat ccgtcagcca caggcccgtc gccgcaggcg
    22921 gagccggcgg ccggccgcgt ccgggcttgg gcggttccgg tggcttccgg aagtcctgcg
    22981 ggcgctgggg gacctcgatc gggtacatcg tcttcgggac catgcggaac aggcccgaag
    23041 tttccaggtc ggcaaaaagc gtctcgttaa aggcgcccat gaagggttgc gcaccgccgg
    23101 agccgcgaag gtccggcacg gcgattgccg cgcgctcccc cttcgtgatc ttgatcagaa
    23161 tgtcgctttg ctgggcggcc acaacaagcg caatgcccag cgcacaagca gtggccgtca
    23221 ggtatcgtgt tttcatcgcc tcaattgaaa ccagaattcg attctcgcat cattccgttc
    23281 gtaacccgcc ggcagcggcg ggaacggact tgcttcatag atcgcccgca gcgccgagta
    23341 gtcgagcgca cggttgccgc tcgactgctc cagccgcacg tcggtagtcg aaccattgcg
    23401 ataaatgacg aacgtaacga tggcgggagg cgcggtttgg agcctggggt ctacgtcgtc
    23461 cgtacgccat ttctgggcga ctttctgctc gagcagatca cgatagtatc cgaaacgatt
    23521 gccgaaagct cctccggttc cgatgccaac gccacccgaa ccggtgcgtc cgactagcgg
    23581 cgaggaaagc ccgcggcccg tgctgctgta gacctgttcc ggaccggtct cgggagtcag
    23641 ccccgaccgc cggctttgcg tcgctctagc tggccgtttc ggcgtgcgtc ccttgatcgc
    23701 caccgtggcc ggatcgggtt ccggcgcctt caccgtctcg accttcgcgg ccggaggctc
    23761 aggcacgctg gattcggtgt cgctcgccag cggattcggc actccccccc gggacggcag
    23821 aggaatctgg ctcaccggtg ttatgccgac cgcacttccg ccaagcgaat ccggatgccc
    23881 ccacgactcg ggcgcccgcc gggggatcag cccctgcaga gcaaggccgg atatcagaac
    23941 ggcatgtaag atcgtcgaca cgccgagggg ccttcgcagc gactcttgcc cctccaggat
    24001 gtcgacgtgg gccttcattt actcaccgta agtaataatt aactgacttt aattctagcg
    24061 cttccgctcc gcctcatcga ccggctgggt aacagcgttc actttgagcc cagcttcgcc
    24121 gagcgcgctc agtacctggg cgatcggatc ccacaccgtg cctttgtccg ctcgcaagta
    24181 gacagcctga gcctctccaa agcgctttcg aacagttgcc gccagctcgt tgatgttgac
    24241 tggttgctca ttgagataca accgtccgtc aacggtaatc gacactaccg ggagctcatc
    24301 ggcgctgtcc ttgacctgac ggaccttcgg gacctccacc tcgaggccaa actccatcac
    24361 gtgtgcagtc agcatgaaga tgatgagcag cacgagcacg acgtcgacga ggggaacaat
    24421 gttgatttcc gacagcgatc cggccccgcg gaagcgcctg cgtccgatgt actggccgcc
    24481 attggaggag ttcgcggaga aggccattcg tcaatcctcg aagttgcgct cagccaggtt
    24541 cataaactcg agggagaagt catccatgcg agcgcccatc tcccggatca cgtggccaaa
    24601 gtggttatag aatacggcgg ctggaatggc ggccgcgaga cccattgccg tggcgacgag
    24661 tgcgtcggag atgccgggcg cgaccgcgcg caggctggat gcgcctgcct ggccgatgga
    24721 atgaaacgcg tcgataatgc cgagcacggt gccgaacagg ccgataaatg gcgagacgga
    24781 agccgtggta gccagccagt tcatgttgcg ctccagttta gtcagttcct cgctggttcc
    24841 aagctggagc gagcgctcga gggctagctt gtttccgagc gtcccgcggg ccttcacctg
    24901 gcgctcaacc tcttcgtagc cgaactcgaa cagcgctacc aacggagact ggcgaaactg
    24961 ctcgctcgca agcgcaacag cctccagtcc gttggccttg cggaacgccc ggagaaagcg
    25021 aacgttcgag gttctcgcct gccggaagac attccacttc gagaagatca ccgcccacga
    25081 aacgacggag aacaccagca gaatgaccag aacggccttg ctaatcagac tggtacgcat
    25141 cacgagatcc aagatgctca cctgaatcca gaaagcggcg gcgcttgaga gtatcgtcaa
    25201 cacctctcct ttctcaaaag accatgataa tgcacccgcc gttcgttcgc gccaccggca
    25261 ttacaacaaa gatggtctat tttgtttttt cttccggcgc tcgcattccc ttataatgcc
    25321 ctcggatgct cgtcgagttg gttgtcgaga attatgcggt catcgagcgg ttacgggtgc
    25381 gctttcatcc ggggctcaac ctccttaccg gcgagaccgg cagcggcaag tcaatcgtgg
    25441 tcgacgcgct cggcctgttg ctcggagggc gcgccgccgg agacatggtg cggaccggcg
    25501 ccgaacgggc gcgcatctcc ggcatcttcg aagccggcgt gtccagaagg ctccggcagg
    25561 tgctcgaagc cgccggagtc gaaatcgaag acggcgagct gctcatcgag cgcgaaatcc
    25621 tggcgggtgg caaatcccgg gcattcgcgg gcagccgtcc ggttactgcc tcgctgctcc
    25681 gcgaactcgc gccgcacctt ggcgacatac acggccagca cgagcagcag cggctcttcg
    25741 acgcagacgc tcaaattgaa atgctggacg ccttcgccgg ctcggccgat ctcgtcgccg
    25801 aggtggcgga tttgtacctc cgctggcgct cttgccggag ggaactcgac gagttggagc
    25861 gtaacgagca ggaaaagctc cgactcgcgg acctctgggg cttccagcgg cgcgagattg
    25921 aggaggcttc acccaaaccc ggcgaagacg ctgaacttga aagcgaacgg cggcgactgc
    25981 tgaactttgc caagctcgag gagaacgctg catcggccta cgaggcgctc tacgagtcgc
    26041 ccgagtcggc gatcgcccgc atgcggcacg ctgcgcgcag gctcgacgaa ctcgctcgca
    26101 tcgacccatc cctcggggcg ctcggtgaaa gcctgaaatc cgcggagttc tccgtcgagg
    26161 aggccgccta ctccttgcga gactacctgt cgcatctcga aggtagccct ggccggctcg
    26221 aggaggtcga gacccgcctg gcagcgatcg aaaaactgaa acgcaaatat gggcaaacaa
    26281 ccgaacaggt gctcgcctac ctcgaagagg tccgcgggca gttggccgca ctcgagaaca
    26341 cggagagccg gcgggcagcg ctcaacgagg aactgcgaag ccttgccgcg gcgtatgaag
    26401 cggcggcggc gcggttgagc gaccgtagga aagacgccgc acacaagctt gagaaacgcg
    26461 tggagacgga gctgtcatcg ctggccatgg agcgcacggc gttccgcgtt cgcgtcggac
    26521 ctgcggcatg gtcggaacat ggcgtggacg aagtctgctt cctggtctca ccgaacgtcg
    26581 gcgaggagcc gcggacactt gagaaagtcg cctccggcgg tgagatttcc cgcatcgccc
    26641 ttgcgctaaa aacgtgcgtg gctgcgggcg ccgggaagac caccaaaggg gagggagcgc
    26701 cgcgtacgct cgtcttcgac gaagtggacg cgggcatcgg gggtcgcgcg gccgaggtcg
    26761 tcggccgccg cctgaaggat ctggccgcct ccagccaggt tctgtgcgtc acccacctgc
    26821 cccagatcgc ggctttcgcc gaccatcact actacgtcga gaagaaggag gcaaacggcc
    26881 ggaccgtggc gacgattctg gagctgaccg gagactctcg cacgcgggag gtcggccgta
    26941 tgctcagtgg cgagaaactc accccggagg ctctgaaaca cgccgagaag ctgattcaag
    27001 ccggctcggc ggggaagcgt ggcgcataac cttgctatac taggagtggt taacctgagt
    27061 taagtagaac gtatctatgt tcgccaactt gtctatcgga gtggccatac tcgtcatttg
    27121 cctggccctc gccgcagttt ttctctggcc ggggccaggg atcaggcgag aactggcaaa
    27181 gcgccgcgaa ttgcacgccc gggcgctgct ggaagacgtt ctaaagcaca ttcttacgcg
    27241 gcggcacgag gggcgtgaag ccagcgtcca atccattgcc ggtcaactca atctgccagc
    27301 ccaaaaggtc taccggctga tcgcgcgaac cgaatcggcc ggtcttgttc gctccgacgc
    27361 cggccgggta caactcaccg ccgaaggcga acggtgggcg ctccaagtgg tccgtgctca
    27421 tcggctttgg gagacgtggc tcgccaatga ggcgcgcatg cccatggcag aagtgcacaa
    27481 gagcgccgaa cgcgccgagc acaagttcac ctcgaagcag atcgatgaac tggacgcgta
    27541 tcttggccac ccccgtcagg atccacatgg cgatcccatt ccgaccgctt ccggcgagat
    27601 tgcatctctc gaggcccggc cgctgaccga atggcctgag gacacgccgg cacagatcgc
    27661 tcacgtcgaa gatgaaccgg tggcggtctt tcggaagatc actgatctcg gtctccggcc
    27721 gggctcggta gttgagatcc gccggcgctc gccggaccag ttcaccatca ccgacgggga
    27781 aaaagagcat acgctcagct tgctcgttgc gggcaacatt caggtgatcc agccggcgca
    27841 gacagccgcc cgccgcgagt cgctggcgcg gctgtcggat ctggtaccgg gcgaggcggg
    27901 cgaagtggcg cttctcgacc aggagcttcg cggctttacc cgccgccggc ttctcgacct
    27961 cgggctgacg gcaggctctc gcgtcaccgc tcacctgacc aatgcattcg gcgatccgct
    28021 ggcctaccgg gtgcgcggca ccacaatcgc cctgcgaaag gagcaggcat cgaagatttg
    28081 ggtcaggaag cacgccaaca cggagaagga ggtgcgcgca tcatgaatct ccccggccat
    28141 gattgcgaca cctgtccggc ccacgcgcaa ctcgcgcaga tgggtatcag cctgacaaag
    28201 tatgaccggc tggtcgcgct ggcgggcaat ccgaatacag gaaagtcctc ggttttcaac
    28261 gccttgacgg gtctgaagca gcacgtcggc aactggccag gcaagacggt gtcccgcgcc
    28321 gagggcggct acgtgttcaa tggacttcgc tacaagctcg tcgacctccc tggcacctac
    28381 tcgctgttat cgggatcacc cgacgaagag gtcagccgga atttcgtcct cttcggccag
    28441 ccggactgca cgatcgtcgt cgtcgacgcg actgctctcg aacgcaacct gaacctggta
    28501 ctccaggtac tcgagatcac cgacaaggtg gtcgtttgcg tcaatctgat ggacgaagcg
    28561 cggcggaagg ggctggacgt cgacgtccgg agcctggcgc gggacttggg agtgcctgcc
    28621 gtgccaacgg tcgcgcggac caaggagggt ctgcacgaac tggttagcac ggtcgcgggt
    28681 gtcatccagg gtgagatcaa gaccagcccg ttccgcccga aacatcaccc ggagcttcaa
    28741 aaggccatcg acgaacttgc acctctgatt ctgcaggcgg tgccgggagt tcccaacgca
    28801 ccttgggtcg cctaccgcct gctcgacggc gatcaccgga ttcgcgaggc tctggtttcc
    28861 ggcgaactgt ccgagttggc ggcaacgcaa ggacagccgc tcgaccggcg cagcagcctg
    28921 atgtcgatcg agggaaggca atgagcgccg gcatgaatgt ctcagaggga gtactggcaa
    28981 gagcggacaa gcttcgcagc gcgctctccg gcgattttcg cgaccgcatg gtggagtcgc
    29041 tctacgccga ggcggatcgc atcgcgaacc gtgcggtgaa gtccggccag gagcgaaagt
    29101 gggacctcga tcagcggatc gaccggcttg tcacctcccc gattttcggc attccattga
    29161 tgatgctcct gatcggcacc acgttctgga tcaccatcat cggtgcgaac tatccctcgc
    29221 aggcgatcgc gacggtcctt ttcgcactcg agaagcgcgg ggcggagctg ttccaggggc
    29281 taggctctcc ctggtggctt acagggttct tgtggaacgg cgtgtaccga ggcctcgcct
    29341 gggtgatcag cgtgatgctg ccgccgatgg cgatcttctt cccgatgttc acgattctcg
    29401 aggatctcgg ctacctgccg agggtcgcct tcaacatgga ttggctgttc cgccgcgccg
    29461 gcgctcacgg aaaacagtcc ctcacaatgg cgatgggctt cggctgcaac gccgcgggag
    29521 tcgtggctgc gcgcatcatc gacagcccgc gcgagcgcct gattgcgatc ctgaccaaca
    29581 atttcgtgcc ctgcaacgga cgctggccca cgctcatcat gctggcgacg attttcgtcg
    29641 cggcgcaatt ccagccggct ttcgcctcgt tcgccgccgc ggcggcggtc accggcgtgg
    29701 tgctgatcgg ggtggtcttc acgctgattg tgtcgtgggc gctttcacgc acgattctgc
    29761 gcggtgaggc ttccgcgttc acgctggaac tgccgccata ccgccgtcca agcgtcctgc
    29821 gcattctcta cacgtcgctg atcgaccgca cgctgttcgt cctgtggcgg gcgatcatga
    29881 cggctgcgcc ggcgggagct gtgatctggg tgcttgcgaa cattaatgtg ggcggcgtca
    29941 gcctggcgcg tcacatctcg gatttcctta atccgttcgg ccacgcgatc gggctggatg
    30001 gcgtcatcct gctcgcctat atcatcgcca tccctgccaa cgagatcgtg gtgccgacca
    30061 tgctgatggt ctacctcggc acaggcatga tgaccgaggt aacgtcgttc gacgatctgc
    30121 ggcggattct agtcggccaa aacggctgga cgctgctgac ggcagcgaat ctcatgctgt
    30181 tctcgctgtt gcacaacccc tgcgcaacca ccatcctgac gatgtggaag gagacgcgaa
    30241 gcggaaaatg gaccgccatc ggcgccctga tgccgctcgc catcgcgttc ctggtgacgt
    30301 tcgcagtggc caccgtcgtg cggttattgg cctgacatcg ctcggccaca ttcccgttca
    30361 ggtgagccga agaagccgag ctgcatttcc gcgccggatc ttttccttga cggcttcgct
    30421 cgcctccaga acgtcgagct tcaaaatcgc cggatcggga tagtgaaacg gcattcccgt
    30481 gccgaatacg acgcggtcct caccgaggtt ctccaggatc tggccaagct cgttcgcgag
    30541 cagcgccgac agcaggcaaa tctcgacagc atagttggcc ggtagaccgt tgttcttcct
    30601 tcccagcacg gagccggtaa atccggagcc gctcatcagg atgaattgcg ccttcggcgc
    30661 ggccttcact accccggcga tctcatcctt gtcgacgtcc ggcacatcga cgagccagct
    30721 tctttggcgc cggtcctccg cgcggagggg aatggaaacc accataccca gctccgccgc
    30781 catatgaaca aggtccaggc acgcggggtc ggtcagcttg tagctgtgcc agcgcgggta
    30841 caggcgcaat cccttcatgc cgaattcctc gtggcaggtc ttgaggtcgt cgcgccagcc
    30901 ggcgtacgcg ggattcagca ccgcgaacgg gatcaggcgg tcccggtggt ccctgatctc
    30961 cgctgcgatc tcctcattcc ccgagtgagc gttccggtaa gtgatggacg ctgcgcttga
    31021 aaccaccgcc cgctcgattc gcttccggtc catcaggcgg agcagccctt ccgccgtgtt
    31081 gttcctcagt tgccggaagg cgaaatgtcc gagatatgcg ttgacgtcga tcatcagacc
    31141 cgcctccggt tgagaatccg ctgcatgttc ccgcctagaa tcttccgtcg gtcagcctcg
    31201 ctgatgtccg cgccgcggat gcggcccacg ctcgccgtga gcgacatgtc gcacgcaaac
    31261 agcaaccggt ccgcgcccag aacgcgcacg gccatctcaa ccgtaccttc gtcgatcacg
    31321 ctgccgctcg tgtccaggta gacattcggc gcgctacgca atgccttgac cgtccattcc
    31381 cagtcaccgc cacccgcgat gtgggcgcag atgagcgtgg cttccggata cgcctggccg
    31441 gcctcggcca ggtgcccgcc gtcgctgatg cgcggctgcg gctccggcag ccaggatgtg
    31501 tggcccgcat gagacaggac aggtacgcgg agttcgatgg cgagttcgat gattggccgg
    31561 aggacgggtt cggtgatccg gtagtcgttg tagagcttga tgccgatgaa gccacggcct
    31621 tcgacgtagc gccggatttc ctcgatcgcc tccttcgtgt agccagggtt gacgaagcag
    31681 tagccaagca ccctgcccgg aaaccggcgt gtggcatcgg ccatccattc attgcactgg
    31741 cgaaaccctt cgagggtggt gggcctctcc ggcggaagga tggaacaaca gatctgatcg
    31801 atgcccagct tgtccgcggc gtcgatcatc cggcgatcgg cctcctccca ccccttctgg
    31861 cgccgattac tcagatgatc gtgggcgtcg atcaccttgc ctgagccggg agtatcctgc
    31921 gcggagctcg cccgcagcat cagtgctgcg cccgctccca gactcccaag aaaggcgcgg
    31981 cgcggcattt tcattgttgt acgttccatg accggttccc tgttgctagg aatcatcgcc
    32041 gaagccaccg ttccagccag ccataggcgc gttccgccgc ctccggcggg aatccgtgcc
    32101 cgccgcggaa gtagtagctt ccaatccgat cctcatcgcc tagcagccgg tagaccggtt
    32161 cgaggcgcgc cagcgcctgt ctggcgctcc aacctgagtt gggaaagact gagtcctcgt
    32221 cagaagtcga aaggaacagc ggccgcggcg caatcatcgc cagcacgtaa tgcatgtcga
    32281 gcggtgtctg gatggtctcc gagtccggca ctccggagta gaaccgcatg tcgatgtacg
    32341 agctcgacag atagaatccg agccgtggca tcagggcggt cgcatgggac cagcggaaca
    32401 cgttgccgtc atgccgaaac gtgtcgaaac cgcaactgct ggcgcctgcg gctatccggt
    32461 cgtcgagcgc catcccgaac agagttgtgt acccgccgtg agaatgcccg atcgagccga
    32521 tgcgcgccgg atcgacgaag tcgagagacc gcaggtagtc gaccccacgg ctgagatccc
    32581 aggccatttt gcccatcatc gaccactcgg gatgtttctt gtagaaggcg atcgcatcgc
    32641 catagtggcc gctggctgga tcgtgacgct cgccgaagca ggcggcgtca taggcgagag
    32701 tcacaaaacc gcgccgaacc agatgtaagg ccatgtgcag ccgggaattg cccgccagcc
    32761 ccgccggttc gttcttgccc tcctgaacag tttgatgggg acaaatgacg gcgggcgctg
    32821 gggcatcaag cttccggggc acgagcagga acgcgggtac gaactcaccc ggctcgctct
    32881 ggtacattac cctccgcctt atgtagcctt cggaaggggt ctcttcgagg gttctcgttt
    32941 cgagcggagg tctggtggcg ggagacggac cgagaatcgc ctgaatgacg ccgagaatgg
    33001 agcgtcgccg gctggaccaa gcgccggcag cattgatact ttgcccattg aatgtcagca
    33061 gatccaccaa gtccgccttg ccggtttggt ctgacacgat ctgaggacga ttctcgccag
    33121 tgatttcatg ccagcggtcc cagctccaga cttcgtaccc agtgactcgc gtcgtttgct
    33181 gggctgccac ggcccccgga accgtccgca gaaaggttcg acgtagcata gcgcgtggac
    33241 tagcgattct actaaaccag cggtgggaga gacacgctcc cggcgtgacc tacggagcgg
    33301 cgggatctgt gcggctgggt ggcggcgcat cgaatgccag cgggaggcca tcgtttttcg
    33361 ccccgtgcca gcgcgtaaac gagtcccgaa tcaggccgaa gatagctact cggttctgcg
    33421 caaccagttt caaccaagcc caagtgtttt tctgcgggaa gtagatgcct cggaagaaga
    33481 gccgtgcaat caggtggctg atccggtacg gtaccggctc gtttcggatt gagttcagcg
    33541 ctcttcgcgt ggccgctggg ctgtaggagt tcctccaggc ttccgtgacc tcgctctgca
    33601 cctcggggat ggtcatcttc atcggcgtgt gcgccatgtg gaacggagcg aaatcgagcc
    33661 agtgcttggg ccgggtcagc cggccttcct tctccagccg gtcatagagc ggagtggcgg
    33721 ggaagggtgt gatctggccg aacaccggca ggacgggcgg ccactggctg atctcgcgca
    33781 acgtgcgttc ggcgacgcct ggaacatcgt tgtccagacc gaagatgaac gaggtgatcg
    33841 cgtagacgtt ccgccgcgcc aggcgttcga gcaccgcgga gtaatcggcc ggtttgttga
    33901 aggtcttgtt gacgctcgcc agattcgcag catcgatgga ctccatgccg atgaagatcc
    33961 acttgccgcc cgaatcggcg atcaggtcca ccagttcgtc atcgcgcagc aggttagcgc
    34021 tgatctgacc gacccacgaa agctgcgctc cggccgcgat gatgtcgcgg aggagcgcct
    34081 tggtgcgctt cgcgttgatc gcgaagttgt cgtcgacaaa gaagaccgcg atcttgccat
    34141 gcgtgcggcg tgagcgcgcc ttcagttcca gcatctcgcg gacaatgctc tcgttggagc
    34201 ggaatcgtat ggaatccccg aagaagcccg tcaccgtgca aaactcgcac ccgtaagggc
    34261 atccgcggcc ggattcgatc gggatgatgt ggaaggtctc ccactccgtc cggaagtgct
    34321 ccattaccgg ctttgcgaag gcgggtatcc ggttgaactg atcgatgttg atcgtgtccc
    34381 aggggatgcg gggatactcc tggagactcg gcttgcgctc ctggccgaat tcgtccgtcg
    34441 gtgtgtagac ttccttcaac tgcccgcgcg cggcgtcttc cacgatgcga ggccacgtct
    34501 cgtcggcttc gccgagcgcc accgcgtcgg cgtggcgggg tccaccgtct cggccgagtg
    34561 cttcgtcggg gatctcggtg acgtgcggac cgcccattac taccggcacg ccggcagccc
    34621 ggaccgcgtc cgcaacccgg taggctttgg cgatcattcg ggtcatcgcc ccgattccga
    34681 ccagactgat tttgttcttt agaacgaacc gcacaagctc ggcatcgctc atcggctccg
    34741 cgttgccgtc aatcagcagg acttcgtggc ccggcggagt cagcgcctgg agcaggaaca
    34801 tccacaagtg cggcatgaag ttgcgggtca cgccgttgtc cgggttgtat agcaggattt
    34861 tcattaagta cctgccaccg gacagttggc cggcgacggg ggtgctaccc agcgcacgta
    34921 tgcggaaaga ccacacccca aggtgtggtc tttcctttat ctcacagttt gagcggctcg
    34981 ctaccagcgg ggttcacggc gccggccaaa gccaccaccg ccgccgcggt ttccgcctcg
    35041 accaccgcca ccgccgccga accggttgcc gccgccggaa cgctcctcgc gcggccgggc
    35101 ctcgttgacg ttcatggcgc ggccgttcag ctcacggccg ttcagtgcct ggatggcctt
    35161 ggcggcatcg tcgctgccgg ccatttcgac aaacgcgaag ccgcgcggct ggccggtgtc
    35221 acggtcacgc acaatgctga cacgttcgac cgtgccgtag gcggcaaatg ctccctggag
    35281 ctcatcttcc gtggtttggt aggaaaggtt tcctacgaaa atgttcgtca tcttctactc
    35341 ctctgagctg cccataggca gttagttttc actggcgagc tcgtgtcggc cggacaattg
    35401 ggcgggcgaa cgtagcaggc atagtacgcc accacccggt ggcgcaacca caagtgtacc
    35461 acgctatcaa gtccggctgt gtcaaggttg tcaagcgaga tagtcggccg cccggacaac
    35521 cctgttctcc cggtacgcca ggttgcctac atgggcggcc tgggccgccg ccaccgccga
    35581 cttcacgtcc gcgttcggct tcttccggct gcggacgcta tccaggaagt ttcgcacgtg
    35641 gtccacagtt ccatcgtgtg tagcgcgggc ggtgatcacc ggctcggggt aggtcgtctt
    35701 ctcggcgggc aggacgccct cggggtaaac ggccagcccg tcgcgcgtga tgcgcatcat
    35761 ggcgttcgtc ccccgaaaca ccagcgtccc tccctcgagg tggcccacca tggttccgtt
    35821 atacacgagg ttgtagtccg gatacttcca ggatgcggtg atggtgtcgg gacactccca
    35881 atacgtgtga tggtagcggt ctcccgtggc ttgcgcggcc agcggccggt ccgactgcat
    35941 gaaccagtgg acgacgtcgc cccagtgcga atgcaggtcg gtgagcgcgc cgcctccgaa
    36001 atcccagtac caccgccact gaaggaagcg gcgctgttcg aatggctgct cgggcgcgga
    36061 acccaggaag cgtttccagt ccagctttgc ggtgtccacc gggctcaacc ggccgaaatt
    36121 gcggatgtag tcctggtacc agtgcgacag caccagcgtg accctcccga gcttgccgga
    36181 gtgcacaatt tcccggccca tgtcgaaaag gtcccagcta cgctgctggt atcccacctg
    36241 cacgatccgg cccgcctctt cgaccgcctt gatcagggac ggtccctcgg caatcttgtg
    36301 gctgactggc ttctctacat acacatcctt cccggcgcgg accgcgtcga tcgtcatcgg
    36361 cacgtgccag tggtcgggag agccaatgaa cacagcgtcg atgtccttct gctcgaggag
    36421 ttcccggtac tcggtgaact cacgcgcgtt gggcgccagt tttgctctgg ccccagtgcg
    36481 ccgcggttcg tacacatcgc agacagccgc gatctccgcc cctccggtct gttcaagcag
    36541 actcagaaga tattggcaac ggccgccggt cccgattccg cccacgcgga tgcggtcatt
    36601 ggcgccgagg atgcggttgt gcgacgcggc ggtagccgcc gcggcagtct tcagaaactc
    36661 acgtcgggtt tcgctcatac tctgcgcgct ccttcagtac ccttgaagat ctcaaatccg
    36721 cccgaaccgg acaaggtata gtcgagagtt acatcattcg gacagctaca aagacacgat
    36781 catgaaacga cctcatcgaa ccaagacatt gatgaccgcg gcagctctgg tttcttcggc
    36841 gctggccgcg tatccggccg atcctgccag gaaaagcccc gaattccgca aagagttcct
    36901 ggaacgattt cagcggactg gactgaacac gactcccggc gacgccatga tgctccgcat
    36961 cctcgtcgag agccgcggcg cgaaacgggg tgtcgaggtg ggagccgcca gcggattcgg
    37021 cgccatgaac atgggcatcg ccttcgagag aaccggcggg catctctata cgctggagat
    37081 cgatcccaag aaagccgagg aaaccaggca aaacctgcga aaagtgggcc tcgaaacctg
    37141 tgtctccgtc gtcgaaggag acgcgctgaa gacgctcccc accctgaccg gcgaattcga
    37201 ctttgttttt ctcgatgcgc tcaagcagga ttacatgaag tacctgaagt tgatcgagcc
    37261 gaagctgaag ccgggcgccg tcatcgtcgc cgacaacgtc atcgtgtccg aaaaagcgat
    37321 ggcggatttc ctggcttacg ttcagacgag ccccaatttc gacaccgtca ttatccgcgc
    37381 gtcatcggaa aaaggcgacg gtatgtcggt aagctacaag atccgctgag gggccggaat
    37441 ctgctctcct ccccgctcgc ttagcctgcg aacttccagc ctttgcggaa cttcggtttg
    37501 aggtagcggt tggcctcgct gttgttcgtg aagcgcttct tgccggcgtc ccaatcgagc
    37561 ttgccctcga acttcatcgc gaccacgcca agcagcatcc actccgcgaa cgggccagcg
    37621 acgctgaagt tcgagcacgc cggttcgcgg cccttgcacg cgcggatcca gtctcggtga
    37681 tgaccgggag atcgcgtcag cagtggcggc ggcagcttgt agtcctgcat tctctcggcc
    37741 gggaccagcc gcgtatggcc gccgtaacat cccgttgtta ccatgccctt gtcgccgatg
    37801 aacaggcttc cgttgacatc atcgtccccc agcatctctc ccgcaggcac cccgtcgacc
    37861 ttgggttgtg cgtccattcc gtcatgccag aagattttga ccgccggcat cggcccgcgc
    37921 gctgggaagt cgaaccggat gattgaccgt tgagggaatg tgtacttgcc cttgccttcc
    37981 tgtttcacgc actccacact gacgggcgca gtcagtcgca acgccatgtt cggcgtaccg
    38041 aggatgtgac acgccatgtc accgatggcg ccgcagccga actccggaaa cccccgccag
    38101 tggtgcggag cgtaggccgg gctaaacggt ctcggctcgg accccccaag ccaggcctcc
    38161 cagtcgatta actccgtaaa tcctgaacct tcatgctacc ccagggggct ctggccggaa
    38221 atcagaaatc cgcgcttgca agacttgcag acctttttat cttgtatata atcaacagtc
    38281 cccttgtgcc ttctgatgcc gtctcagaca cggggaagtg gcggcccgct tctgagttgg
    38341 ttggagggaa ttatctgtgc gttttccatc atgaattgct gcgccccgag taatgcgccg
    38401 tgtcgcgcct ggcttcctcg tgggcctcgc ctgcaccctg tccgccttcg cgcagggagg
    38461 cgccatcgcc gtcaaagagg tggcaactcc gaacctgggc gaagcctatg ccggccaagc
    38521 ggcggtcgcc gataacgcct caaccgcgta tctcaacccc gctggaatga cccggctaga
    38581 gagctccgag acgctgatcg gcggacaact ggcggcgctt ggcgtccgtt ttcagccgaa
    38641 ttcgttcagt acgatgaagg gctccgacgg tggcgacatt cgggagtacg cactgctgcc
    38701 cggcacctac gtcgtccgcc gccttaccga caggctacag ttcggattca gtttcaacgc
    38761 tcccatcggg ttgtcactcg actattccga agattggatc ggccgctacc tctctcggca
    38821 catttcgatg gcaatcctgg aattccaccc gtccgtaggc gtccgcgtga accgctggtt
    38881 gtcgctcgga ttcggcgcgg ccattcagcg agcctcgctc acacagaaac aggcgggcaa
    38941 taattcgctg ttcgtctccg gctaccccga cggtctcatt cacgccagcc accatgactg
    39001 ggggttcgga acccagtaca gagccctgat cgaaccctcg cggcggaccc ggatcggcgt
    39061 aacgtatcac tcctacatca agtacgtcat gaagggaacg atcgaaatgg aggacgtcac
    39121 gacgccccta gcgaccaccc tcgccagaaa cggaaagtcg gacaaccgga tgcggatgcc
    39181 gcaggccgcc gacgtcagtc tctatcacga cgtcaccgac cgcttcgcgc tgctgaccga
    39241 cgtaggctgg accgcctggc ggcatttcgg cgagaagacc tacacgctgc caagcggcgc
    39301 gaagaacacg gtccatcgga aatggcgcga tacgtttagg atcggactcg gcatgcgccg
    39361 caagctcaat gaccgccaca tgctacaggc cggcgtctcc tacgacagta atccggtgtc
    39421 gtattgggac cgcactccag accttcccgt tgaccggcag atccgctacg ccacgggctg
    39481 gcagtacctt tggagggtgg gactgattct cggcttcgcc gtctcatacg ccgatctcgg
    39541 tgcagcccgg ctcgacacac cttacaatcc cttgcaagga aagctcgccg ggcggtatga
    39601 gctggcccga ttgccgttcg tttcgctcac aatgcagttc aagtagccgc agacggtcgt
    39661 cgccttcgcg ctcgcttccg cgacggacac ctgatcatcc gcgctacgct gaatcgagac
    39721 gcgttgatga ggcggcggtg caaacctgag atcatgagcc cggccgggga ctggccgcag
    39781 ttgcgcgcgg ccgtcgaagc cggcgccgac gcggtctact ttggtctgaa gcacttctcc
    39841 gcccgcgcga aagtgggttt cccgctcgac gagctcccgg acgtgatgcg cacgattcac
    39901 cagcgcggcg tgcgcggata tatcaccttc aacacgctag tcttcgaaca cgaactcgcg
    39961 gaggccgcgc gggcgatcgt cgcgatcgcc gaggcaagcg cggacgcgat catcgtgcag
    40021 gattacggcg tcctccaact cgcgcaccag atcgccccgg acatggtgct gcacgccagc
    40081 acccagatga gcatcaccaa ttcggacggc gtgaagctcg cgcagagctt cggagtcagc
    40141 cgcgtgaccc tggcccggga actgtctctg gacgaagtcc gcaccattgt cgatcagtcc
    40201 gggtgcgaga tcgaaatttt cgtccatggc gcgctctgcg tcgcatactc cggccagtgc
    40261 ttctcgtcgg aagcctgggg cggacggagc gcgaaccgcg gccagtgcgc gcaggcttgc
    40321 cggcttccgt atgagctgat tgtcgacggg cggctgcagc ctctgggtga cgcccgctac
    40381 ctgctctcgc ccggagacct ttacacgctg agccagattc ccgagatcgt cgaaacggga
    40441 gtcgctgccc ttaagatcga aggccgttac aaggacgcgg actacgtggc gctggccacc
    40501 cttgcgtatc gcaaagccgt ggacgaggct tgggcgggcc ggaagtccag aatcagccgg
    40561 gaggaggaac ttcagctcga acaggtgtac tcccgcggca tgggtccgtt cttcctggtt
    40621 gggacgaacc accaggaggt agtccggggc cgggctccgc gccatcgtgg cgtgttcatc
    40681 ggtcgtgtta ttcaagtgga atcagaccgc gtgctcattg agcccggtaa cacccatcga
    40741 atcgcgcctc tgaagcccgg tgacggggtc gtctttgacg ccgccgactg gcgcagcccg
    40801 gaagacccgg aggaaggcgg ccgcgtgtac gaggttctgc cggcgccgga cagcaggctg
    40861 gcactacggt tcggaaacgg agcgatcgat ttctcgcgga tccgccccgg agatctactc
    40921 tggcgtacgc atgacccgga cctcgatcgc gtcgtcgggc cttacaccgg cgcgccagcg
    40981 cccgtttcgc ggcaacccgt acacgtcaag gtgatcgccg tcgagggagc gccactcgtc
    41041 gctgaatggt cgctggtaga ccagccgggc gcgagcgtga ccgtgaagtc cctcgcttcg
    41101 ctcgatcggg cgcagaaccg ccgtctctcc gcggaatccc tgcgtgggca gttcgggcgc
    41161 ctgggcaaca cgccttacga gctcgccggc gtggcgctcg acgtggaggg cgcgccgttt
    41221 gcccctgcgt ctttgctcaa tcagttgcga agggaggcgg tggagcagtt gcagtcgctc
    41281 cagagccggc cgcgtcacgt aacggtcaac gatccgtcgg cggccgtttc agcggtgctc
    41341 gggcagaggt ccgctgacgc gccggccgag gacgcgccgc ccgaactgca tctgcttgtt
    41401 cgcacgccgg agcagctcga agccgcacag gcgctcaatc ccgccagcat tacgctcgac
    41461 tatctcgacc tctacggtct gcgcccgtcg ttggagcgca tcaagtctgg cggcctggag
    41521 gcccgcgtcg cgagtccccg cgtcctcaag cccggcgaag ggcgcatagt cgatttcctg
    41581 ctcagcctgg aatgcgcaat tcttctgcgg tcagcgggaa tgctgcacgc tctgcgcgcg
    41641 cgagagcacc cgccgctcat cggcgatttc agcctgaacg ctgcgaatgt cctggccgcc
    41701 agaacctatc tggagttggg gctttcccgg ctgacaccca cgcatgacct gaacgccgag
    41761 caggtcgcag acctcgcaag gagtgccggc gcggagcgca tcgaggcggt tgtctaccag
    41821 cacctgccgg tcttccacac cgagcactgt gtgttctgcc ggttcctttc gaacggaacg
    41881 agctatcgcg attgcgggcg gccctgcgag aagcaccggg tagcactgaa ggaccaggcc
    41941 ggccgcgccc atccggtgat ggcagacgtg ggctgccgca acacggtgtt cggcgccgaa
    42001 gcgcaggagg cgagccgcca tctcgaagcc tggaggcggg ccggcatccg ctacttccgt
    42061 ctggagttcg tgcacgaatc cgccggtgcc gtccgccgaa ttgtccgtgc gtttcagcgc
    42121 gcactcgccg gcgccacccc acccacgcaa ctcgcagccg agctgaaatc catcgtcccc
    42181 gaaggcacca ccgaaggcag cctcttcgtc ccggacggct acctgttctt gccggtattg
    42241 cggtagcagc ggggccgagc tcggatcaac gacccggggc agacttatca acgccagccg
    42301 ccccaagaga agagcgtcct tttcgggaca ctcttctctt ccgggcgaga ttggctttcc
    42361 gccagcgatt agaacaagaa cttcaccgtc atctggatga ggcggcggcc ttccttttcc
    42421 gtgacaatgc cgaagttcgg gttgtccact tttccggtcg ccgggttgaa gatgaggtta
    42481 aggttgttcg agctggggaa ggaccacagc gggtggttca ggaagttgta cccgtcgaag
    42541 cggaactgca gcttcctcga ttcactgatc gagaagttct tgaagagccc gagatcccag
    42601 ttgaagaaag ccggtccgta gatgggcggt agcaccgttg gaccgttcgt cccaatgctc
    42661 gtgggcatcg caaaacaact ggcgttgatg tactggttct tgccgaggcc cgagcgtggg
    42721 tcacatgtca gcagcggcct gagctggaga ttgggcgtcc ccaggattga cacgttgctg
    42781 atgttgtggt cggtgcccag aaccttggca ttgtttagat tcatgccaaa gttccggttg
    42841 ctgctgttgg gccacaggtt ggcgccgctc tggacctggg tgatgccgga gagttgccag
    42901 ccgttgagga cgccgcccgc cactttgctc ttcaccgggt tgcccagttc aaacgagtaa
    42961 gcgacgttga ataggtgcct gcggtcgctc ggcagaactc cgtagttgtt gcgcaggttg
    43021 aactggtcgt cgaaattgat cagtcccatc gccttgccaa acgtgtaatt cagattgacg
    43081 ttgtaccggc ccttggccct cacccacgtc acttgcagcg agtcgtagtt cgaatacccg
    43141 ttgaaagtgg caagattgag gtccgagaac cccttcaaag gacggaagtc gttcgccgta
    43201 agtgtgttcg ggtccactcc accattgttc gacgcaagca tcgctccgac cgggacgaga
    43261 ttgatgttca tggtgttgaa gcccaggctg ccgccattgc cggagctcgg gatatcccgg
    43321 gtccggttgc cgacgtaggc caactccagc aagccagacc aaggcgttcg ctgcgcaacg
    43381 gtgaagctaa agctgtcggt atacggctgc ttgtcgctcg tgccgtctac ggcggccggt
    43441 gaaagcgcct gactggtgaa actcaacgtg tcgagccgcg ctgccgttag tggctcgccg
    43501 tcgacggtgt tgctgagatt gatggtctgg acacccgccg cgacatcgag gccattagtg
    43561 aactgtccgg cgtgaaagta gaaccggccc cagccacccc gcaagacggt ctggcctttg
    43621 ccaaaaagat cgtaagcgaa accgagacgc ggctgccaga agacggttct cgtcggaaat
    43681 ccgcctagtg gcacactggg atcacgtgcg tgccacaaga atccgcaata gtcactgggt
    43741 ttacaggtcg gactgtactt tgagtagtcg aagatcgaaa acccgaatcc ctcgcggtct
    43801 atccatggct ggaagtgcgt gaggcgcacc ccaaactcga ccgtgagttg cctgctggcc
    43861 ttccaggaat cctgcacaaa gccctcgtat gtgttgtacg cgatgtcgtt gatgcggttg
    43921 aaggactgct cgttgtacga attcagcacg cccaggagca ggtcggcata cgtagatccg
    43981 gtggtgtttg ggttagcagt ggctctcacc gacagcaacc cgttgctatt gccgttggcg
    44041 ggctgggcat tccggatcca ctcccagaag aaccccgcct tgacggtgtg ggtgccgaac
    44101 acctttgcga ccgtgtcgct gacgctgggc atgtacttgt tggcatacag acccgacgag
    44161 gggccgcccg cttcgaagcc gccaggattg aacatgaacg cggcttcgct gggtccgtaa
    44221 tcgccgaagg acggaatctg ggcgacgccg ttcttgtaca gccccttgta gttgtagccg
    44281 accgccgcgc gatccacctt tgaaggatcg tcgaagacat tcgggaagcc gatgaaggtg
    44341 tagccgaaga cgaactcgtt cgtcatcgac gggctgaaca catgggtcag cgatgccgaa
    44401 atcgaatcgg aacggttctt gcccaggatc ggtgtcggat aaggaacctg atcggtgttg
    44461 cgccaccaaa gcccgaccgg gaaccgctgc gtttcgcgct gcaagttata gcgcacgaag
    44521 agcttggtgt tatcgctgat gttgtaatcc acccgagaca tccactggat gttgttctgg
    44581 ttgaagttca gcgccttgac gaaattgtat ccccctgtcg cgtttggatc cgcgttggca
    44641 gcgggatata gttttgcgag agcgaccatg ttcggatcga gctgcgaagc cggaatcctc
    44701 gcccctggcc acttcaggag cgacgcttcg ttgagctgct ggggagctac gccaagggcc
    44761 gtctttgttc cgagcttcgc caattcctcc ggcgtgaaat tgccttgcag catgtccggg
    44821 gtcggaaccg tagcacggac aaggccggta tccagagtct ggtagaagta ctcgtacccg
    44881 gtgaaaaaaa acagcttgtc gcgattcttg ttgaagttcg tgccagggat caggaccgga
    44941 ccgccgatcg tgccgccggg aaagaagtac ttgttctccg gcgccttctg gccactctta
    45001 ttgaacagcc agtcgttggc gttcaacgag aagtgcctcg cggagaagaa cgccgagccg
    45061 tggaactcct tgcccccggc ctttgttact gtgctgacaa ttgccgggcc cttctggttc
    45121 tcggcgctga aattcgaggt gagcactttg aattccgaaa ccatgtcggc gtttggattt
    45181 accggcgtgt cacagttaca accggggtcg gaaacgtgcg cgccgtccgc cgtgatatcc
    45241 agcgagttgc tcggtaaccc attgtaggag taagcgttgt tcagcgggct ctggctgccg
    45301 gcatcgccgt tggcgttgat accaatggtc tgaccgtcat agttcgactt gttccgcacg
    45361 ccgttttgga tgccgaaccc cggcatgatt cggatgaact gagccgcgtt actgcccaag
    45421 actacgaagt tttcgagctc cttcgtcgtc agcgtctgcg acttttcgcc ggaatccacc
    45481 gggacgatcg actcggcaat ccctgtgacc tcaatggctt ctgtggtggt gccgacagtc
    45541 aaggtaacgt tcacgttgcg tctttcgccg ccctggaggg caagacccgt cgccttatag
    45601 gggacgaagc cctgcatctc caccgtcagc gtgtacgtga agttgcccac agccacggaa
    45661 gtgaacgtga agaaaccttc gtcatttgta actgtttcac gggtggaccc ggatgcctcg
    45721 ttgacgagtt tcactttcgc gttgggagca acggcttggg actgatcagt caccacacca
    45781 gttagagttg cgaaaaggga ttgggccgac atttccgcgg ccgacagaaa caaaagcgaa
    45841 agtactgcga ggaagcaggc tctcatcttc ccctccctac ccatccagtg gcaacgaccc
    45901 cagacaaatg agggaaaact ccactccctc atcaagaatt ttgggaatag tgtaatccta
    45961 ctagtgaggg caaagcaata gttattttct gccgtgaggt aattaattga ttctattgat
    46021 ctgttacaag ggttacctcc tggccgctct ctgcccgcca cgattaaaac tagttcatgg
    46081 gaccgccggg atcgttactt agcccggggc tcgtcgattt tgaggaactg gttggccggg
    46141 atgtcctcca acacctgctg catcccgctg ggccagcgga tttcgaggga gctgatttta
    46201 gtttcctggc cgaggccgaa gtgtacgcgc ttgtcgctcg aagacatgaa gccgacgctg
    46261 acggcgacat ggttgtgaag gcgacgcccg gacgcggtgg tgagtttcac tttggctccg
    46321 atggcgtccc gcgcgctctt gtgccccacc aagtccagga cgagccagcg gttaccgctg
    46381 tcggcggtgt tccttagaat gcggggcgac tggttcaggg acgtcacgac aatgtccacg
    46441 gacccgtcat tgttcagatc gccaaaggcc gagccccgct ggtagccctt ccggaggaag
    46501 tcccctccca tctcttccga aacgtccacg aacgtccttc cgccaacgtt ttcgaacatt
    46561 gtgtcgtgct gcggcgagtt ttcgttaact tcgtccacat ccccgttggc cgagtagatg
    46621 tccttccatc cgtcgttgtt gtagtcgatg aacccgttgc tccagccgga ataaggcatg
    46681 ctcagcgcct gaatcttcga tggaaaggaa tagaacacga acagatcgcc gcgattgcgc
    46741 aggagctgcc agatctgggt cataaggttg ttgtagaaga tgtccacgtt gccgtcgttg
    46801 tcgaaatcct tggcgtcgga acccatcgaa gatcccgtgt gggcgctctc gttgtaggcg
    46861 actcccaact ccaatccctt ctcttcgaac gttccgttct tctggttgat aaaaagggaa
    46921 ttgggctcgg tgtcgttcgc aatgaatatg tccggccatc cgtcgttatt gaagtcggcg
    46981 atcgagatgc ccattccttt gccgggcgcg gcggcgagcc ccgacttctt ggtgacgtcc
    47041 gcaaacttgc cgccgtccag gttccggtaa agccggggag gaacgctcgg gtaccggcgt
    47101 ggatcgcagt agtaatcctt actgtccatt gtgcagcgga agtcgttctt tgggctccag
    47161 aatgtgtaat cggagacaat gagatccagc aggccatcgt tgtcgtaatc gaaccaggcc
    47221 gcgaccacac taagagtgtt ggccggcttg ccaccgatgc ctgaagcctc cgttacatcg
    47281 gtgaaagtcc cattcccatt gttgtggtag aggacgtttc tagcggcgct cgcgatgaac
    47341 aagtcttcgt agccgtcgtt atcgtaatcg ccggccgcga caccgaagtt gtaatctagg
    47401 tgatctccag tgaggcccgc cctggcggta acgtcttcaa acgttccgtc ggcccgctgc
    47461 cggagtagac agttgtagaa agaaggatcg gttttcttta attcgggaag atgggcgccg
    47521 ttggtgaaaa agatatccat ccggccgtca ttgtcgaagt cgaaaatcgc cacgccgcca
    47581 cacattggct ggggaaacca cttgcgtccc tcggagctgt tattcgtttt ataggagatc
    47641 ttcgagcggg aggcgacatc cacaaaatga ggccgtccgg cggccttggg gggccgtcct
    47701 gacagcagca ccccccataa gcatgcacac aacacgagcg ccgcaagaac cgccatgcgt
    47761 atcatgcggc cctcccggga tgccgggcgc gatccgattg ggaaagcgtg ccgcatggta
    47821 gactgaggcg tccagacgtg aaagtcctgg tttctattgt agtcggagcg ttcgggcttc
    47881 tggcaggttc cgtagtgagt gtggtctttg aggatatcgc cggccggtcc ggcctcgttt
    47941 tcgtctcgaa cagctccccc accccgaaca agaatcagcc cgaaacgatg gtcgccggcg
    48001 tcgcgctgct ggactacgac aacgacggtt atcttgacgt ttatttcgtg aacggcgcga
    48061 cgataccgtc gctgaagaag gaaacccctc agttctggaa ccggctctac cacaacaacc
    48121 gggatggtac gttcaccaac gtgaccgaga aagcgggcat gaagggcgac gggtacggca
    48181 tgggggcggc caccggcgac tacgacaacg acggctggac cgatatctat gtggtaaacg
    48241 tgactcggaa cgagctcttc cgcaacaatc gcgacgggac gtttacggac gtcactgaca
    48301 aggcgagtgt gagtggcggg attctcgacg gcaagaagat gtggtctgtc tcggccgctt
    48361 ggctggacta caacaacgac ggtctgctcg atctctttgt gtcgaactac tgtaaatggg
    48421 aagtcaacaa agacccattc tgcggcccca agccgagcct gcgggcctac tgccatcctc
    48481 agaactacgg agcgctgccc aacactctat accgcaataa cggggacgga acattttcgg
    48541 acgtatcatc cgagaccggc atcggcgagc atcccggtaa gggcatggga gcggccatcg
    48601 ccgactacga cggggacggg ttcatggata tcttcgtcgc caacgacacc tatcccaact
    48661 tcctcttcca caacctgaag ggaaagaagt tcgaagaagt cgcgctggag ggcggcgtcg
    48721 cttactccca agccggacat cctctctcgg ggatgggtgc cgatttccgc gatgtggaca
    48781 acgacgggcg cgcggacatc tggcacacct ccgtcgagaa cgaatccttt cccctgtatc
    48841 aaaaccgggg agggggtcaa ttcgtggaca agacgatccc aggtctcctt gcgggaacgc
    48901 ggcacatgtc cggttggtcg aacggggttt tcgacttcga caacgacggc tggaaggatc
    48961 ttttcgtggc ccgcggaaac gttctcgaca acatcgcgca gttctcagac cgcacttacg
    49021 gcgaacagaa tactgttttc cgcaatctcg ccaacggtaa atttgaggat gccagcagca
    49081 atgcggggac tgatctccag aaggctgcgc ctcatcgtgg ggcggccttc ggcgacatcg
    49141 acaatgatgg ccgagtggac gccatcgtga cggtcctgaa cgacaaggcg aaatacttcc
    49201 acaacatttc gcggaacagc aaccactgga ttctgctgaa gtttaccggc cggaaaagca
    49261 accgcatggc attgggggcg cagatccgca taacagccga cgatggcctg aagcagtaca
    49321 acgaggtcac cactgcggtc ggctacgcct gctccagcga ctctcgggtg catttcgggc
    49381 tgggcgcatc caagagtatc cgtgagatcg aaatcaggtg gccgagcggg atccggcaaa
    49441 cactcacgaa tgttgccgcg gaccagattc tggaaattca ggagacggcg aactgaactt
    49501 cgcttctact tttcgatttt gcccctggcc tggctggctt cctgttcggc tcttaacttc
    49561 tgcaccagcg cccgctcgcg atctccgtcg gtcttgcgct tcaggcgata gtaaacctgg
    49621 gcgagggaaa cgtgcgcctc gagaaactgc ggtgcctgtt tcacgacctc ttccagagtg
    49681 gcgcaagcct tttctagctt gccagcggcg aggtcggtgg cgccgacctg atataaagcg
    49741 cgcaggtccc ccgcgcgcac ccggagagcc cgcgcgagca gtccttgcgc gcgatcgaac
    49801 tcgccgttct ctttgagcag tactgccagc ctcaggttcg attcgtaatc gttggggttc
    49861 cgctccaatt ccttttcgaa ggcgtcacga gctgccggca tgttgccgct gagcatttga
    49921 gcccggccga ggtaagagta gacgtccgga agtttgggct ttagcttcac ggctttctgg
    49981 aaatctgcga tggcggcggg atagtcgagc gcttcgagct tggccgttcc gagaagaaga
    50041 tgtgcttccg ccgagtcgcc gtttcgcaag atgcggtcaa tcatctgttg cccgcgttcg
    50101 atctgcttgt cgcggagcaa tgccgttcca agcagatacg caatggcaag gtcattctgg
    50161 ttctgctttt caagtggagt gagtaggtcg accacgcggg tgttttctcc ctgccggagc
    50221 cagcagtcgg cgagcagcag cgtgacttgt tggttggcgg gatcggcggc gtgcaaggag
    50281 gacaactcgc gggctgcttc tgagatttct cccgccttgt agaaagccag agcgagattc
    50341 agccagatac gggggtcctt cggcccgagc tttaatgctt gctgatactc ggtgatcgcg
    50401 tccgtgtagc gtccagtgct tgccagcgcg gcgcccaggt tcgaccgagc gtcgcgagcg
    50461 tcgggccgca gcttcaggta ggcccgatat tgctgaatcg ccgtctccat gttaccagcc
    50521 cgatgcgcgg caatggcctg ctgcagaatc cgctccggag tctcttgagc gaatgcgaaa
    50581 agccaaaaga cggacaacgc cggcagaaag ctcaagcggc gtggcattga aatctatggt
    50641 agatggaacc ctgaaattcc ggcaagcggt cgccacagat gtaaaaaaag acctggccgg
    50701 gatgtctgtt gtcgatttga gctgtccccg agcgactaca tggtaaggac agcggttccg
    50761 ccgcaaatga agcagcgcag aggaaccgct gagaatcgag acgagagggg aatcggaccc
    50821 gcgccgctga ggtcgaggcg acgaaggggc tctcggaggc ccagtggaac ttcaagcgtg
    50881 ggccggagcg ctggtccgtt gcggaggtcc tggagcatat cttgctcgtc gaggacttcc
    50941 tgttggagaa cacgtcccaa gaagtgatgc aggcgccggc aggcaaggcg gaccgcgact
    51001 acaagagcac ggacaagatg gttttgagcg ctatcgccga ccgcacccat aaagcgcagg
    51061 cgcccgagcc ggtgattcct aagggacgct ggtcacccta ggagtggctt gaccattttc
    51121 tgaagagccg ggagaggacg atagcattcc tcacgtccac acccgggttg cgggagcatg
    51181 tcacggactc gccgcttggc caacccctgg acgcctacga gtggcttctg ttcatctccg
    51241 cccatagcga gcggcacacg aagcagatcc tcgaggtcaa ggccgatccg aattttccga
    51301 agaactagtg acgggagtcg atcatgcaga ccgaaccgca gaaggaacat cagtggttac
    51361 agaagctggt tggcgagtgg acttatgaaa gtgacgtggc gatgaagccg ggcgagccgc
    51421 cggcaaagtg cgagggaacc gagagcgtgc gatcactggg tcccctttgg atcctggccg
    51481 agggggaggg cgagataccc ggctgcggcc acgccacgac gatgatgacc cttggttatg
    51541 acccggagaa aaagcgctac gtaggcactt gggtcggatc gatgatgagc cacctttggg
    51601 tttacgacgg cgctctggat tcagcagggc tggcgctggc attggacacc gagggtccca
    51661 gcatggctgg cgacggcaag atgacaaagt accaggacgt gatccagttc aagagcaacg
    51721 ataaccggct gctcatatcg caggtgctcg gtgatgacgg caagtggcac cagttcatgt
    51781 cgacgaccta tcggcggaag aagctgtgac ttgccgcccc ggaattacct cctccacgcc
    51841 gcggcgatca gagccgctat aaagacaact tggagcacgg cccgcagggg caggctggtc
    51901 gcctttctgc cgccggatga cgatcgctca tgccggggtg cgccggcctc tggagaccga
    51961 cgcacctcga ccgacggcga cttgcggccc ggcacgacca cgcagctaag cactggcttt
    52021 ctgggccgct ttgggttgag tacgcatttg ctgcatgaat tgccgcatgg cggcaacatg
    52081 accgggcgag cggcgctcgg accaggattc gaaacgctgc gccgcttttt ccatgtgtcc
    52141 gaggaactgc ttgtccgcga accgttcccg agcctgcggg acgatggact tgaggagctc
    52201 ccaaacaccg aaaaactcgc cactggtctc gaaaaagaga tcttcatgga gcaagccata
    52261 gttaagcaat gcacaggctt gctcccagta gctcatcacc attcctgcga acgcgccgga
    52321 ttccgtgcca ggagctgcga tgcgcatggc ttcctcgaaa gtgtccacga tgtaattctt
    52381 aaagaaccag tcccgtgctt ggcgcagcct ggcctcgcgg cgcaaatcat agacctgcag
    52441 gtgaagttgc gcctgttcgt gcgtgggttt tgtttccatt gtttcttgtc tccttttttt
    52501 gctgtcaatc aaccgcgttg agcagagagt ggctccaacg cagattctga atccgttcta
    52561 tctgtgaatc gaaaggcatt tgcccatatc gacaatgtgc gagaagtgtt tacaggtggc
    52621 acatttccgt tcgattactt cggtgagttc ccgatccgtt cttgcgtgca gattcgccat
    52681 cgtggcggtt cacgtagtct ttctcacggt gtgctgggcg gcaccgacca gcccccaccc
    52741 aacgctttgt aaagcgagat taagcgggta accacagcgg tttggctctg agcgagctga
    52801 tcctcgtgtt cggagagagc gcgctcggcg tcgagcaccg tcaggaattc cgccagcccg
    52861 gcccggtact gcgcctgcgc gagttcgagt accgtccgat cggctcggat gactcgttgc
    52921 aggcgctcgc agcgttcctg ctcgttggaa tagccgacaa gcgcgttctc cgtctcttcc
    52981 agcgcggtca agacggtcga ccggtgggtg gcctccgcct gcttcacgcg cgcttcctgg
    53041 tggcgcgcgc cattcacgcc ctcagtggca ggcggcaggg aacgtttgtc aaactgaact
    53101 gcgccgccat tcccacgggc ttgctcgaaa gcgagacgtt cggccatgag aaaggcgcgt
    53161 ttaccggggc gatcgcgcag cgcgtcggcc ggttcgagct cgcgcacaag gggacagttt
    53221 tcctcgacga ggtcggcgag attccgctcg agctgcagac caagctgctg cgcgtcttgc
    53281 aggaacggga gttcgagcgg ctcggcagct cacggacgat gcggacggac gcccgggtca
    53341 tcggcgcaac caaccgcaca cgggaggaca tggtacggga tcacaagttc gttccgacct
    53401 ttactaccgg ttgaatgtct tcccaattac cgttcccccg ctgcgggagc ggccggaaga
    53461 cataccacta ctcgtccgct acttcgtcca gcagtttgcg cggaggatga acaagagcgt
    53521 agccacaatt cctgccgagg acatgcgcgc actggtgagc tacccgtggc cgggaaatat
    53581 tcgcgaactg caaaatctga ttgagcgcgc ggtaatcctc tcctcgggac ctgttctcgc
    53641 gattccagtg caacaactcg aaaaggcagc cgcattgtca gcgtccgcgg cagcctccgc
    53701 accggccccg gacgtgatta cgctcgaaga cgccgaacgg gaggctattc tgcgagcgct
    53761 gcgaaactcc ggcggccgcg tcagcggcca gcacggggcc gcgactgccc tcggcacgaa
    53821 acggaccact ctgcaggcca ggatgcgtaa gctcggcata aacccaagag gtatttgagc
    53881 cgttacgtat tctgaccggt gagaagcgtc ggccgctttc agcggcacgt ggaacacaac
    53941 cgagtggcgt ggaattgccg aagagcatgg atgcagattg ttttgtcttc ggatagctgg
    54001 cggtgagggt gggattcgct atcgcgaccg ccgcccattc taaacacgtt gccctgtttt
    54061 caatacgttg gaggcgtggt gctgaccagc cctttaggtg tgttgctcct gctttttctg
    54121 cctacgcagt tttctccaaa tcgggatgtg gaccgcgtga taccgccatg cgacgccgat
    54181 ccagatcagg aggcgttgca tcaatgagtc cgtgccaaca tagtcagctc ggatctctcc
    54241 ggccataccg gccgaaacat aggtgagata gcgcgtaccc acaatgctgg cat
//
LOCUS       DATIFQ010000183         3142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_444490, whole genome shotgun sequence.
ACCESSION   DATIFQ010000183 DATIFQ010000000
VERSION     DATIFQ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444490"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VLE22_08250"
     CDS             complement(<1..341)
                     /locus_tag="VLE22_08250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB14436.1"
                     /translation="MEKRALAAVCAAGFAFSANYTNHAPMVPALQAEFGFNQASAGLL
                     TTGIFLTHALMQVPGGRLADRLGAARVLAAALTWVSVGNFGIAFAGAYWQFLLWKVFV
                     GIGTGTCFTAGA"
     gene            635..3064
                     /locus_tag="VLE22_08255"
     CDS             635..3064
                     /locus_tag="VLE22_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HSB14437.1"
                     /translation="MTMSAKHNAVICLLLLPAWANPQTGAAGSLPPNQWTELANDPAG
                     ARRGCGVRFASKAGAFFLWGFMNDDPDLEGRLMKVSEYDMVLFDPSSGRWRNHLPLEK
                     QEEWSKQLPMAYSPGTYMGITTGSRRTIMRGEAEAYDGVPRPALNIVFDQVAYRPVDN
                     SLLYFTGGLTAAYDLERRTWRDLQPAHSPPPVLGGAIAYDPVRDEMVLAGGGHIAEAG
                     SGRALKGYTGTWVYSYRDNDWQQLTLRRQPPPRMNTRMVADTKNGVLVVFGGDAQNAH
                     LADTWIFDLKSRTWKVSGARSGPPPRAGHFTVFDPQTGLVIIGGGYNRTDLRDMWSYD
                     AARDRWQRLAGDVPTAFYISADIAPDERLIVLVTNERRPDLRMNCNTLYPVRRTFGYR
                     LDVKTMLHPDQAMQAHMPMPKRAPEEMKGTEPDRSREAAQSERLRMMPVNRWVHLADP
                     GRVAPLRTWGSATFDIDRGQILNWGGGHCGYGGSDVDAYVVDAHTWRGEPEPEYPGRS
                     WDKGVSLAGVTFGGNPWNEHGRKIFAYDPVSRKMIMARPVGLTEGYQPEWLKAETSAC
                     PRCATWSYDPETQTWERLASAPPGVTALVTTPRGVIGATVAWGSRLDRRGYLLPGSND
                     AEDNPLFLFDAAKNEWIRLGDPQPSPGNLYEMTSVAYDTRRDQVILHGGGENRDQLWI
                     FNLKTRRWENMEPKVLAPAGAQPPVCTRESVYLPGDDVVLIYGPSRGERTKPALWEYS
                     VAENSWRLVEIPPMTEVEPRQRASQNRAMVYNPKRDLVLLVLGSRGDAGPAEVFAMRY
                     QRSQAASASHE"
BASE COUNT          627 a          963 c         1021 g          531 t
ORIGIN      
        1 gcaccagccg tgaaacacgt gccagtcccg atgccgacga ataccttcca taggaggaac
       61 tgccagtagg cgccggcgaa ggcgataccg aagtttccca cagacaccca agtcagggcc
      121 gccgccagaa cgcgagccgc gcccagacgg tccgccaggc gtccgcccgg tacctgcatt
      181 agggcgtgtg tcagaaaaat gccggtagtg agcaggccgg cgctcgcctg gttgaatccg
      241 aattcggcct gaagcgcggg caccatcggt gcgtgattgg tgtagttggc cgagaaagca
      301 aatccagcag cgcaaacggc ggcgagcgca cgtttttcca ttcaagttac ccgttgagag
      361 attcttccga atccggtgaa gccgggaact cgccagtata tagcaggacc atgcaccagc
      421 attccaacgg gtagcttagg tcccgccgga gttgtgatgc agggccgaag tcggttgtac
      481 aggaggtccc cggcgcggag ttgctgccca cacgcctttc ggctccgcga cgaacatcgg
      541 agtagcgatg cggatcgtcc cgtccggaag tgtaggagtg cccggtctca gacttctggt
      601 tgtgatagga tcgcgcagaa actgatccgg gggtatgaca atgtccgcaa agcacaacgc
      661 ggtgatctgt ctgttactgc tgccggcttg ggcgaacccg caaacgggcg cggcgggcag
      721 tctgccgccg aaccagtgga ccgagctcgc gaacgatccg gccggcgccc gccgcggttg
      781 cggggtccgc ttcgcatcga aagccggggc gttcttcctc tgggggttca tgaacgacga
      841 cccggacctg gaaggccggc tgatgaaggt cagcgagtac gacatggttc tcttcgatcc
      901 gtccagcggg cggtggcgga accaccttcc actcgaaaag caggaggagt ggagcaagca
      961 gctacccatg gcgtactctc cgggaactta catggggatc actaccggca gccgccgcac
     1021 catcatgcgg ggcgaagccg aagcctacga cggcgtcccg cgacccgccc tgaatatcgt
     1081 gttcgatcag gtggcgtacc gtcccgttga caactcgctg ctgtatttca caggtggact
     1141 caccgccgcc tatgacctgg agcgccggac atggagggat ttgcagccgg ctcattcgcc
     1201 gccgcccgtt ctcggcggcg caatcgcata cgatccggtg cgagacgaga tggtgctggc
     1261 cggaggcggc cacatcgccg aagcgggttc cgggagggcg ctgaagggct atacgggcac
     1321 gtgggtatac agctaccggg ataacgactg gcaacagcta actttgcgcc ggcagccccc
     1381 tccgcgcatg aacacgcgga tggtcgccga cacgaagaac ggcgtgttgg ttgtgttcgg
     1441 cggtgatgcg cagaacgcgc atttggccga cacctggatc ttcgacctca agtccagaac
     1501 atggaaagtg tcaggcgcgc gttccggtcc gccaccccgc gccggccatt tcaccgtctt
     1561 cgatccacaa acggggctcg ttatcatcgg cgggggatac aaccggaccg acttgcgcga
     1621 catgtggagc tacgacgcgg ccagggaccg gtggcaacgg ctggccggcg acgttcccac
     1681 cgccttctac atctctgccg atatcgctcc cgacgagcgc ctcatcgtcc tcgtgaccaa
     1741 cgagcgcaga ccggacttac gcatgaactg caacactttg tacccggtcc ggaggacgtt
     1801 cggttaccgc ctcgatgtga aaaccatgct ccatcccgac caggccatgc aggctcatat
     1861 gcccatgccg aaacgggctc cggaagagat gaagggcacg gaaccggata ggagtcgcga
     1921 ggcagcccag tcagagcgtc ttcggatgat gcccgtcaac cgctgggtac acctggcgga
     1981 tccgggccgc gttgcgccgc tgcgcacttg gggcagcgcg acgttcgaca tagaccgcgg
     2041 ccagatcctc aactggggcg gtggtcactg cggctacggc ggcagcgacg tggacgctta
     2101 cgtcgtcgat gcccacacgt ggcgtggcga acccgaaccc gagtacccgg gccgcagttg
     2161 ggacaagggc gtgagcctgg cgggagtcac cttcggtggc aacccgtgga acgagcacgg
     2221 gcggaaaata ttcgcctacg atcctgtcag tagaaaaatg atcatggccc ggccggtagg
     2281 gctaacggaa ggataccagc ccgagtggct gaaggccgag acgtccgcat gcccgcgttg
     2341 tgctacctgg tcctacgatc cggagacgca aacatgggag cgcctggcat ccgcgcctcc
     2401 cggcgtcacc gcgctcgtca ccacgccgcg gggagtgatc ggggccaccg tcgcatgggg
     2461 atcgcggctg gaccggcgcg gttatcttct tcccggcagc aatgacgcgg aggacaaccc
     2521 gctcttcctc ttcgacgccg cgaagaacga gtggatccgg ctaggcgatc cgcagccttc
     2581 tccagggaac ctctacgaga tgaccagcgt ggcctacgat acgcgtcgcg atcaggtcat
     2641 cctgcacggg ggcggagaga accgggatca gctctggatc ttcaatttga aaacccggcg
     2701 ctgggagaac atggagccga aggtgctcgc gcccgcggga gcgcagccgc cggtgtgcac
     2761 gcgcgagtcg gtctaccttc ccggcgacga tgtcgtcctg atttacggtc cctcgcgtgg
     2821 ggagcggacg aaaccggcgc tgtgggaata cagcgtcgct gaaaactctt ggcgtctggt
     2881 cgagattcca ccgatgaccg aggtggagcc ccgccagcgg gccagccaaa accgagccat
     2941 ggtatataac ccgaaacgcg atctggttct gcttgtgtta ggcagcagag gggatgccgg
     3001 accggccgaa gtgtttgcca tgcggtacca gcgctcccag gcggcttccg cgagccacga
     3061 ataagttgct gctggtcgca ctatccaaag gagaatcaag tttctgtggt acatagggcg
     3121 aaaggagaga acgagtgttc ta
//
LOCUS       DATIFQ010000184         2839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2359795, whole genome shotgun sequence.
ACCESSION   DATIFQ010000184 DATIFQ010000000
VERSION     DATIFQ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359795"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..425)
                     /locus_tag="VLE22_08260"
     CDS             complement(210..425)
                     /locus_tag="VLE22_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSB14438.1"
                     /translation="MHNEFTAVFERDGEWHVAYCPEIPGANGQGRTKEDARRSLAEAI
                     ALILEDRREEGLRGVPPDAERETVVVK"
     gene            complement(442..1083)
                     /locus_tag="VLE22_08265"
     CDS             complement(442..1083)
                     /locus_tag="VLE22_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB14439.1"
                     /translation="MSIAGGDRHGEVRHYGVIASDLEPLDKVVRALRAPNRTLHFVYE
                     AGPCGFGIFRHLTKRGEDCMVVSPSMTPKRSGDRAKTDRRDSLALARLHRAGELRPIY
                     VPDDTDEAMRDLVRAREDAVAVGVQAKYRLKAFLLRQGRRYPGRDGWTLAYRRWLTDL
                     SFPRAAQHMALHEYRDTVDQTERRLERLTEQLRQLAPTWRWVPVVAALPFAVS"
     gene            1291..1812
                     /locus_tag="VLE22_08270"
     CDS             1291..1812
                     /locus_tag="VLE22_08270"
                     /inference="COORDINATES: protein motif:HMM:NF028114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5071 domain-containing protein"
                     /protein_id="HSB14440.1"
                     /translation="MRIEHKQRLLRKELRKLLPSNKEDVTAIEAIAAHGYPAVDPILL
                     DLLKWIRVEWWPVSKPACQFLVSIGPRLAPQVSEVLGSRDDLWKAVVLREIVSGWPTE
                     DVRALSSHLFLIATDGQSWGADLLALRLLAQHGIAESDWIAGWLEFKREHHEKRLADI
                     TEISGILKSRGAA"
     gene            1923..2348
                     /locus_tag="VLE22_08275"
     CDS             1923..2348
                     /locus_tag="VLE22_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14441.1"
                     /translation="MTTMNADVPHVSGGRRALGYVGAVLLLLIIPVKLLRYSSATASV
                     AVGVAPSLLGPSGLLFLLLSSPGKVSRCSVAQTTMCVGAISVGLELVQLLPRPGILQR
                     VRYTFDILDLLATLAAVGISFVIAKVVLGGRGHRATGYG"
BASE COUNT          500 a          872 c          884 g          583 t
ORIGIN      
        1 gttagacaga ctcgacgtca gccgtggccg atttccggaa cagagaggcc acggcaaatt
       61 tttcgcgccg ggttgttgct gatctcgttg tgccggggaa tcgcctcaac cgcgccccgt
      121 ttgtggattg gtccagaggg aatgcgcggc accttcgcgc ttgagcgagc acccgtgccg
      181 gcgcagatgc tggagcaggg cggcgcgttt cacttcacga cgacggtttc acgctcggcg
      241 tcgggtggca cgccgcggag accttcctcg cgtcggtcct cgaggatgag cgcaatcgcc
      301 tcggcgaggc tgcggcgagc gtcttccttg gttcgacctt ggccgttcgc gccgggaatt
      361 tcgggacaat aggcgacgtg ccactcaccg tcgcgttcaa aaacagcggt gaattcattg
      421 tgcatcgaga tggctccgcg tctaggatac ggcgaacggg agcgccgcca caacgggcac
      481 ccaccgccag gtcggcgcca gctgccgaag ttgctcggtg agccgctcca gtcgccgttc
      541 ggtttgatcg accgtgtcgc gatactcgtg cagcgccatg tgctgcgcgg cccgcgggaa
      601 actgaggtcg gtcaaccagc gccgataggc cagcgtccat ccgtcgcggc cgggatagcg
      661 gcggccctgc cgcagcagaa acgccttcag tcgatacttg gcctgtacgc cgaccgcaac
      721 cgcgtcttca cgcgcgcgga ccaaatcccg catcgcttcg tccgtgtcgt cgggcacata
      781 gatcgggcgg agctcgccgg cgcgatgaag gcgcgcgagt gccaggctgt cgcgtcgatc
      841 cgtcttcgcg cgatccccac tccgcttggg cgtcatcgac ggactgacca ccatgcagtc
      901 ctcgccgcgc ttggtcaagt gtcgaaaaat cccgaatcca cacggcccgg cctcgtagac
      961 gaagtgcaga gttcgattcg gcgcccgcag ggcccggacg accttatcga gcggctccag
     1021 atcgctcgcg atcacaccgt aatggcggac ttcgccgtgc cggtccccgc ccgcaatcga
     1081 cacgtcgatc gttccttgtg gacatccaac cccacgaaca tgctacggtt cctcatgacc
     1141 gacctcctcg cgtgtggcgc tgtgcgccgt tgatcagacg tccgcataat ccacgagaac
     1201 tcgaggacgg tcggtcactt tttcttactt gctcggaacg ctgtcacgag ccgtgacagc
     1261 catgttgtct aggcagacag gaggcgacgc atgcgaatcg aacacaagca gcgccttcta
     1321 cgcaaggagc ttcggaagct gcttccttcg aacaaggaag acgtgacggc catcgaggcc
     1381 atcgcggcac acggatatcc tgctgtggat ccgatccttc tcgaccttct gaaatggatt
     1441 cgcgtcgagt ggtggcctgt atcaaagccg gcgtgtcagt ttctcgtttc gatcgggcca
     1501 cggttggcac cgcaggtgtc ggaggtgctc ggttcgcgcg acgacttatg gaaggcggtg
     1561 gttttgcgag agatcgtcag cggatggccg acggaggacg tccgcgccct gtcgtcgcat
     1621 ctatttctga ttgcgaccga cgggcaatcg tggggggcgg atctcctggc cttgcgcctc
     1681 ctcgcgcagc atggcatcgc cgagtctgac tggattgccg gttggcttga attcaaacga
     1741 gagcaccatg agaaacgctt ggccgacatt actgaaatct cagggatcct gaaaagcaga
     1801 ggagctgcct aaaagcggct gcacccgaca gcggccgctc gggatctatc gtaataaaaa
     1861 aacgttaatg gcggcccgcc gcgggtgagc cgccatccgt tatgctctct gaatcggaac
     1921 cggtgacgac tatgaacgcc gacgtgcctc atgtgagtgg agggcggcgt gctctcggct
     1981 acgttggcgc ggtgctactc ctcctcatca ttccggtgaa gctgcttaga tactcctcgg
     2041 cgaccgcgag tgtggccgtt ggggtagctc cgagcctcct ggggccttcg ggcctgcttt
     2101 tccttctcct ctccagcccg ggcaaggtgt cacggtgctc ggttgcccag acgaccatgt
     2161 gtgttggtgc catttccgtg ggtctggaat tggttcagct acttccgcgt ccgggcatcc
     2221 tacagagagt gcggtacacc ttcgacattc tggatcttct cgccacactc gcggccgtgg
     2281 ggatatcgtt tgtcattgcc aaggtggtgc tgggtgggcg cggccatcgg gcaacgggtt
     2341 acggttgaga caggctggac ggggtgctat cgcatggtcc gtttcggtaa gctgccgcag
     2401 ggcacggaat tacttctgca ccccctcaat ttctgttgca cgagtgcaac gtcctttgcg
     2461 gttagtggcc tttcccgcgt ccgcccggtg cttaccggaa ccacgtccgt cgcacatgct
     2521 cgacaatcgg caagaccacc tcgggccgcg cgccgatccc ggcagtgttg agcatctgtt
     2581 agagctttgg gtactgccgg acgaacttgc gcgcgcgcgc gatcaggctg gacgagaaag
     2641 tcggtgagtc gatgaaatct ggcgttccgt gactgagaat cacggtgtcg gcagttcaat
     2701 tctgcccctc ggcaccattt aaatcgcagc atccaaaggt ctcagcggta tctcagaggc
     2761 gggtccagct atcggaagtt ctccaccagc atgaggtcgg tgccaggctg atcggactgc
     2821 acgtacaaga gctccctcc
//
LOCUS       DATIFQ010000185         3949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1211124, whole genome shotgun sequence.
ACCESSION   DATIFQ010000185 DATIFQ010000000
VERSION     DATIFQ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211124"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VLE22_08280"
     CDS             <1..262
                     /locus_tag="VLE22_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14442.1"
                     /translation="AAQEGRFEARLEVACRTAQDASVLSFQLEGVTRLLRSLLNREKQ
                     TPNPGDLSGLLAEGSFHHQDRRVLGRWPVQREFLERLTGGSL"
     gene            264..1208
                     /locus_tag="VLE22_08285"
     CDS             264..1208
                     /locus_tag="VLE22_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HSB14443.1"
                     /translation="MGFGSPRGVLCAGNIVHDTLVRPVDRLQWGTTTWVQEIHTSLGG
                     NGAATSFTLGKLGVPVCLLSMVGNDPSGDELLHRLEFAGVDVSRVTRSEAPTPATIAL
                     VHPGGDRLFLHRPGASGVAFREPIEFTPEILRGISHFHLANVFALPLLRPNAAGTLRR
                     AKAAGLATSLDTGWDALGRWMEDLGPCLPSVDVLFVNESEARMLSGRPGIEEAAAALL
                     NSGAGRVVVKLGAQGCAVFAGGEEFCVPAFPIEVVDTTGAGDCFAGGYLAALARGLSH
                     GSAARHANAAGALSASHLGGVDGILSWEEMERWMGNGD"
     gene            complement(1260..3116)
                     /locus_tag="VLE22_08290"
     CDS             complement(1260..3116)
                     /locus_tag="VLE22_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HSB14444.1"
                     /translation="MGEPSHFSDSRIASQLEIAARLPGEPGRDALGEVYQSLVPRAQR
                     HATGEYYTPYWLASLLLDEAGYEGDTSARLLDPACGSGVFLLVAIERAWRRGAALGQS
                     PEETARGVLSGIRGFDVNPLAVQAARANYRQALTAAGWEARDDEIPVFRRDALLEPAD
                     GGTFDLIAGNPPWVRWDHLPAEYREATLPLWKSYGLFSLKGFEARLGGGKKDLSMLFT
                     LAAADRYLKPGGVLAFLITQEAFKTKGAGEGFRRFRLPDGTPLGVIRAHDFTGVRPFG
                     GAANKTAAVLLRKGEETVYPVPYVVWTRHNKELERTELEARPMGSLYGPWQTLPAGEM
                     GTDSHFATAAKWWSVPISPPNYRAMLGANANPYGVFWLEILAGAQGGTVRVRNMAERG
                     KRPIAPVEAEVEAELVYPAVRGADIARWSAGPGVHVLLVQDPAARRGYAEATMQERWP
                     LTLRYLRGFREELLRRALYRKYHEEAGNPFYSQFNIAVETFSPFKVVWKRMSNDLVAA
                     VISDWDGPLGRRRMIPLETTSFIPVENAAEAHYLCAVLNSGPVREFVKSFSSAGRGFG
                     TPSVIRHLRLPRFDAGNPVHRELAEISEQHHRPGADEGAGQKRLEKLMIEKL"
     gene            complement(3120..>3949)
                     /locus_tag="VLE22_08295"
     CDS             complement(3120..>3949)
                     /locus_tag="VLE22_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HSB14445.1"
                     /translation="YILSQEEVFAKIQETIDLGGTGILMQGGLHPDLKIEWYEDLLRS
                     IKQRFAIHLHCFSSPEILNIAEVSGLSVRDTIARLRDAGLDSIPGGGAEILDDGVRQR
                     ISRLKCTTQEWVDVHRTAHALGMRTTATMMFGCGETLEQRVNHLEIVRQIQEDTGGFT
                     AFIPWTFQPHNTSLGRFVKEEATAVEYLKTLAVSRLYLENIRNVQSSWVTQGIKTCQI
                     GLRFGGNDVGSIMIEENVVSAAGAHHRSNEEELRRIIRDAGFVPKQRDTLYRTYFLN"
BASE COUNT          642 a         1395 c         1175 g          737 t
ORIGIN      
        1 tgcggcgcag gagggccggt tcgaagcccg cctcgaggtc gcctgccgga ctgcgcaaga
       61 cgcctccgtc ctctcgttcc aactcgaagg agtcacacgc cttctgcgca gcctcctcaa
      121 tcgggagaag cagacgccca acccgggcga tctcagcggt ttgctggcgg aaggctcatt
      181 ccatcatcag gaccggcgcg ttctcggccg atggcccgtc cagcgcgagt ttctggaacg
      241 cctcaccggc ggttcgctct gacatgggtt tcggctcgcc ccgcggagtt ctctgcgcag
      301 gcaacattgt tcacgacacg cttgtgaggc cggtcgaccg gctccagtgg ggcacgacga
      361 cgtgggtgca ggaaattcac acgagccttg gcggcaatgg cgccgccacg tcgttcactc
      421 ttggcaaact cggcgtgcct gtgtgtttgc tcagcatggt cggtaacgac ccgtcgggag
      481 acgaactgct ccaccgtctc gagttcgccg gcgtggacgt gagccgcgta acacgatcgg
      541 aggcgcccac ccccgcgacg attgcgcttg ttcaccccgg cggcgaccgc ttgtttctgc
      601 accgtcccgg tgcgagtggt gtggcattcc gggagcccat cgagttcacc cccgaaatcc
      661 tccgcgggat ctcgcatttc cacctcgcca acgtcttcgc gctgccgctc ctgcgcccga
      721 acgcggccgg aaccttgcgc cgggcgaagg cggcgggtct cgctacatcc ctcgatacgg
      781 gctgggacgc acttggacgg tggatggagg accttggccc ctgcctgccg tctgtggatg
      841 tgttgttcgt caacgaatcc gaagcgcgga tgctctccgg acgccccggc atcgaagaag
      901 ccgcggcagc gctgttaaac tccggagcgg gtcgcgttgt cgtcaaactc ggcgcgcaag
      961 ggtgtgccgt gttcgccggc ggcgaagagt tctgtgtccc ggcatttccg atcgaggttg
     1021 tcgacacgac aggcgcgggc gattgcttcg ccggaggcta tctcgccgct ctcgcgcgtg
     1081 gtctctccca cggatccgcc gcccggcacg ccaacgcggc cggcgctctc tccgcctccc
     1141 atctcggcgg cgtcgacggc atcctctcgt gggaggagat ggaacgttgg atgggaaatg
     1201 gggactgacc caccatttcg ccgctgttgc gaaatggctg tctgtcccca tttcttccct
     1261 cacaacttct cgatcatcag cttctccaac cgcttctgcc ccgcaccttc gtccgcgccc
     1321 ggacggtggt gctgctccga tatctccgcc agttcccgat gcacaggatt gcccgcgtca
     1381 aaccgcggca acctcaggtg gcgaatcact gacggtgttc cgaaccctct cccggcggac
     1441 gaaaacgact tcacgaactc ccgcaccggc cctgaattca gcaccgcgca gaggtaatgc
     1501 gcctccgcgg cattctccac cgggataaac gaggtcgtct caagtggaat catccgtctt
     1561 cgtccgagag gtccgtccca gtccgaaatc accgccgcta ccaggtcgtt gctcatcctc
     1621 ttccagacca ccttgaacgg ggagaacgtc tccaccgcaa tgttgaactg gctgtaaaac
     1681 ggattcccgg cctcctcgtg gtacttgcgg tacagcgctc gccggagcag ttcttcccgg
     1741 aacccgcgca gatacctgag tgtcagcggc cagcgctcct gcatcgtcgc ctcggcatac
     1801 ccgcggcgcg cggccgggtc ctgcacgaga agcacgtgga cacccggccc cgccgaccac
     1861 cgtgcgatgt ccgccccgcg cacggccggg tacacgagtt ccgcttcgac ctccgcttcc
     1921 accggcgcga tcggccgctt ccctctctcg gccatgttcc ggactcgcac ggtcccgccc
     1981 tgcgcacccg ccagaatctc cagccagaac accccgtacg gattggcgtt cgcgccgagc
     2041 atcgcccggt aattgggggg agaaatgggg actgaccacc atttcgccgc cgttgcgaaa
     2101 tggctgtctg ttcccatttc tcccgccggc agcgtctgcc aaggaccgta caaggatccc
     2161 atcggacgcg cctccagttc ggtcctctcc aactctttgt tgtgccgcgt ccacacgacg
     2221 tacgggacgg gatacaccgt ctcctcacct ttcctcaaca ggaccgccgc cgtcttgttc
     2281 gccgcgcctc caaacggccg gacccccgtg aagtcgtgcg cgcggatcac tccgagtggc
     2341 gtgccgtccg gcagccggaa ccgccggaat ccctcgccgg cgcccttcgt cttgaacgcc
     2401 tcctgtgtga tcagaaaggc cagtacgccg cctggcttca gataccggtc cgccgccgcg
     2461 agcgtgaaca gcatcgagag gtctttcttg cctccgccaa gccgcgcttc gaaccccttc
     2521 agcgaaaaca gcccgtacga cttccacaga ggcagagtcg cttcccgata ttcggcgggg
     2581 agatgatccc agcggaccca cggcgggttt cccgcgatca gatcgaacgt gccgccatca
     2641 gccggttcaa gcagggcatc gcgcctgaag accgggatct cgtcgtctcg cgcctcccac
     2701 cccgcggccg tgagcgcttg ccggtaattc gcccgcgcgg cctgtacggc cagcgggttc
     2761 acgtcgaacc cgcgaattcc cgacagtacc ccgcgcgcgg tttcctccgg ggattgcccg
     2821 agcgccgcgc ctcgccgcca cgcccgctcg atcgcaacga gcagaaaaac cccgctgccg
     2881 caggctgggt caagcaggcg cgcgctcgtg tccccttcgt atccggcttc gtcgagtagc
     2941 agagacgcca gccagtacgg tgtgtagtat tcgcccgtcg cgtgccgttg cgcccgcggc
     3001 acaagggact ggtagacttc gccgagcgcg tcacggcccg gctctcccgg cagtctcgcc
     3061 gcgatttcaa gctgtgacgc aatccgcgag tcggaaaagt gggagggctc tcccatctct
     3121 caattcagga agtacgtgcg atacagcgta tcgcgctgct tgggaacgaa cccggcgtcg
     3181 cggatgatcc gccgcagctc ctcttcgttc gagcggtggt gcgcgccggc ggccgagacg
     3241 acgttctcct cgatcatgat gctgccgacg tcgttgccgc cgaagcgcag tccgatctgg
     3301 cacgtcttga tcccctgcgt gacccacgac gactgcacgt tccggatatt ctcgaggtac
     3361 agtctgctga ccgcaagagt cttgagatac tccaccgcgg tcgcctcttc tttgacaaag
     3421 cggccgagcg acgtgttgtg cggctggaag gtccacggga tgaacgccgt gaaaccgccg
     3481 gtgtcctcct ggatctgccg cacgatttca agatggttca cccgctgttc cagcgtctcg
     3541 ccgcagccga acatcatcgt cgccgtggtg cgcatgccga gggcgtgggc cgtgcggtga
     3601 acgtccaccc actcctgggt cgtgcacttc agccggctga tccgctgccg gacgccgtcg
     3661 tcgagaatct ccgcgccgcc gcccggaatc gaatcgaggc ccgcgtcgcg cagccgcgca
     3721 atcgtgtccc gcaccgacag gccgctgacc tccgcgatgt tcagaatctc cggcgacgag
     3781 aagcaatgga gatggatcgc gaaccgctgc ttgattgagc gcagcagatc ctcgtaccac
     3841 tcgatcttca ggtccgggtg cagcccgcct tgcatcagga tgcccgtgcc cccgagatcg
     3901 atcgtttcct ggatctttgc gaagacctcc tcctgcgaca gaatgtagc
//
LOCUS       DATIFQ010000186        54265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1211958, whole genome shotgun sequence.
ACCESSION   DATIFQ010000186 DATIFQ010000000
VERSION     DATIFQ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 54265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54265
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211958"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VLE22_08300"
     CDS             complement(<1..1122)
                     /locus_tag="VLE22_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14446.1"
                     /translation="MRALLFLLFLAVNALAQAPGVRLWAVSENVRVSPVTGNLIEAQP
                     LIHKDYPTGNPRNGNIAWDAASKTVRLKAARNEFTAFQLIIEVVEPQAGFDVKFSKLE
                     QLGGARIEGRNIAIFKEWYTEVRRPSTGYEKTSLGRDWYPDALMPYRKTNWLPDFPFS
                     IPDLYNNIPYQRNHAIWVDVFVPNERSAAPPGPYTGTLEVTWDGGRDSVNVALDVWDF
                     ALPQENHLKGDVWNGSIKNMPPAEELAYYQLAKQHRFLPLVYAYRPGLKVQGTKAELD
                     WTEYDKRIANYLDGSAFTDKHGYWGPGYGVPVSHMMLPFDIERHGNPANAWPLALPKE
                     GRTPEYDAVWKDVGRQVREHLDRDPHARKVMKVAFLNGLD"
     gene            1165..2367
                     /locus_tag="VLE22_08305"
     CDS             1165..2367
                     /locus_tag="VLE22_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSB14447.1"
                     /translation="MTRIFLLLILSCCLPAQEQRTESFLGNLAPIIRSIHQERGFPMS
                     YADRKGLPVDQWRSRGRAEVQRALSYSPKTVPLDVKVESTVERDGYEIRRITFAASPH
                     YRVPAFLLVPKRGKGPYPGVVALHDHGGWFFHGKEKLVRMEGEHQSLKDFRERYYGGR
                     TFADELAKRGFVVIVPDAFYWGERRLQYAQPPADFQKRLAGLDPASTEYVLAVNSYLR
                     ERNTELNTWLSFCGTSWMGIVVHDDRRSVDVLASLPEVDAKRIGCLGLSGGGYRSTYL
                     TGMEPRIRASVIVGWMTSLPTTLDITYSVHSALFDAHGAHANLDHPDIASLAAPDCAT
                     FVQNCARDRLFTREGMESSAEKIRAVYSDLKHPERYQARFYDVPHQFNVQMQEDAFAW
                     LEKWLGGR"
     gene            complement(2389..3690)
                     /locus_tag="VLE22_08310"
     CDS             complement(2389..3690)
                     /locus_tag="VLE22_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14448.1"
                     /translation="MDTSDSARRQFLKAAGAAFTTSIFTGSIRGANDRIRGGFIGTGV
                     MGSENLGVAARQPGVEIAAVCDVFQPNMERAAAIARRQNHQPKEIRDFREIIADKSID
                     FVCISTPDHWHPYMEVEACKAGKDVYVEKPVCLGIDEGQKMVAAARKYERVVQAGTWQ
                     RSGAHFQKAAEIVRSGQLGKITFCRSWIYENAPQAGIGNPPDGTPPKDLDYQLWLGPA
                     PDRPFNPNRFGVYPHSYSFFRWFWDYAGGQLTDSGVHMLDILQMALDDPMPKAITALG
                     GKLWFTDNRETPDTMLVSFEYPGFIGSWEHRCNNTEGMTSRLMGVNFHGTRGTLYVDR
                     NVCRVTPEKGSDLEAFEMKRVADPHPLHWVNFLECVKTRQRPNSDIEKCYRSSATCLL
                     GNASYRSKLRLDWDDQKQTVLQQEALQYLLRPYRAPWKLEV"
     gene            complement(3715..5748)
                     /locus_tag="VLE22_08315"
     CDS             complement(3715..5748)
                     /locus_tag="VLE22_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HSB14449.1"
                     /translation="MMRYVLTILLALAAAAGSARQPFHDRTHPSKVFGQPRYYRIFLP
                     PAYETSGKAYPVIYYFHGHSDRYTLERYDNGTDTVPKITDFVAKHDVIVVAVDGFVAK
                     NYTGFYGGSPWDLLENGGDNDFGEYFQELVAHIDSTYRTLTDRRHRATSGLSMGGFMS
                     LWTSARYPHLIGSASAFNPGHEFYVGDKGRRTLWRLKDHAANHGHTMVRLIRASGDYI
                     SQYHEEMRDVYARAHEVDFEYRQDEYHRHWATSIGETFDFHMRAFAKSALDNVPEVFS
                     HANPYRKFEVWGYRVEAEGNEPGITLLEGVRQEGLRVTTRRWEPDGPPVRDRRITITT
                     PPLYQAGRAYKLFDHDLSAGKTNTREVTPDTAGRMSFTVDGAGHQVSFSGPGTGAEPP
                     VLLPVTPKDRLRLPPGTEVRMPIRIYNPRGEAMTDVKVAVSSEYPTVRLVAETAAVPK
                     IEPGDFVDLSDRLRATFTAGTGHFAPARLTVTTTFDGWHETSKNVDVLIIPEAIPVPL
                     AVEILDGRTMTLNVFRQKGNQGGGGSISRTVTEGKGNGNGILEPGEEATIWVKLAYGM
                     DPFDKNNWYRTKVYTDSPHVTEIGDIQEQKQLEWTSSKERTSLIRLSPDAPAGAKILL
                     LLDNESWSYHYTPDVRYGTEKLYQAFQLHSRHLHRLELAVPRNPARPAARRVN"
     gene            5777..8671
                     /locus_tag="VLE22_08320"
     CDS             5777..8671
                     /locus_tag="VLE22_08320"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HSB14450.1"
                     /translation="MIRIGIILVSFLLCGSVGAADLWELANSKRDVHRFSTLFTAQDV
                     RDRLSTGDSIDAAIDWCKRTAVTRVYIESFRDGYQAERQTLLHAKERFQKAGIDVSGC
                     ITTTGVGKRSTGWRPLSCYTDKPTQQKIKSVFEFTASIFDEIMIDDFWFTDCTCAECE
                     AARKARTATVGDKAYPVPGDTWEDYRCELMVRLSRDYMLAAAKKVNPKARLIIKYPQW
                     YDRFHDRGYEVARQTADFDRIWVGTETRDRDKGGVVPYEAYFIMRWLGRLGGSKTGGG
                     WYDPFDTTGQTYIEQARQTVLGGARESMLFCYGGLLRNTGPANVEELRGHIPELFVIA
                     DEVGKRQPAGIAAYKPPNSHPEKEQFIFDYIGMLGLPLVPCHEFPEDAQAAFFSVHAL
                     KDPDFAEKLSRLIGRGRPVLLTDGLVQGLGGKVKLDVENVQILPVGGDPKSLLKLSRE
                     KLDVMREPLLRPFRVAFSAPNEAGLYLFDDGSWVVENFTGQEASVDLNEKKLKIAPRE
                     WTYEWRAPSPRDTVVIRPVEIHDVLVNPGMGIQTFQRFNGDPPLRDMTWSEEGPTRPS
                     PVPTAKPDYPGSSIAYFRWYWIQIEPKEGQFRWEIVDNAIAEAQKRGQKLDFRVMPYD
                     EGHPLPEWYRNTGARRANKPTDPDGQIWSPDSGDPLYLKHWNRLTRELGKRYDGHPGV
                     NSVDISTVGYWGEGWGPYLPDWNAQQALIDAYLQSFRKTPLLMNFDVTEALAHATKNG
                     AGWRLDCWGDMGNYWESWGTYLSHMFDTYPQTLVRAGAQEAWRSGPVSLETCWVPPYW
                     KAHNYNIDYIMEQALRWHVTSVNIKSARIPPEWKGKFEEFQKKMGYRFILRRLEYPKR
                     VKAGAKMPVNMWWLNAGVAPAYDPYDLALELRSGNTGSVIRLPADVRKWMPGDSLVEG
                     DVSFPASLKPGEYRVRVGLLDPRTGNPAVKLAIEGRQPDGWYDVGSIRVE"
     gene            complement(8679..9308)
                     /locus_tag="VLE22_08325"
     CDS             complement(8679..9308)
                     /locus_tag="VLE22_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14451.1"
                     /translation="MSVRIIDAAANSSRRLLVGAILVGIAVVYALYEGSVTYPPGVLV
                     SEDPSQSPAESVAPWEYKGLTIKPLASFRLRARVLRSERYWFDGGAKVAPVDLALGWG
                     PMSDQRVIDRLSIWQGQRWFRWMPKDNQPPIPMTEIANHSSNMHMIPSTDQIKDQLLA
                     LRNGQLVGLGGYLVRVSGKDGWSWTSSLSRTDSGSGSCELVWVEYVDPR"
     gene            complement(9310..12201)
                     /locus_tag="VLE22_08330"
     CDS             complement(9310..12201)
                     /locus_tag="VLE22_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14452.1"
                     /translation="MAADTWVSMHRWEGPKSDRNDALENHGADAALVIQGRTAFALLQ
                     FDVSAARGLTVEKATLRIHRKPAPVPLHTVGLSTISGNGPWSEPTVNYFFPGPEKQAW
                     SYPASDLVDVTFAQGGSLYSYERARDAGGDWWEASVPPALVHALIGGDQYGLMLTDEK
                     GQTQTRHEISSRESGNPPVLIVEGSRRDRTAPGTVRSFRTGTVIDSSPAAARKLSRST
                     LRPGSVILHFGGAGDDAGQGVATGYEVRYSETPIATVSFDSASRAPRWMLDPLAPKPN
                     PFATSNSLRDEVNAVVEQLKPGQVYYFAARAIDEAGNAGPVSALGSYRAYSPEFPKLP
                     PGDETAGAAFQPPAADAFSVWAVPETLKIDPRTGELIEKDDFAGHRSRNSIWNASSAT
                     VRLTGARNEFLGFHLAVESKQPLAGIEARVTKRLFSESTLPAIFKETGAIQLYREWAV
                     ADQPKRGIREWYTDALVPLAGPFDVPSKDNPVPEQTVQPIFVDVYIPHDAKPGVHRGE
                     LLVSAGGTERRVALQVEVLPLTLPDKLNFIVDLNCYSSVHSGYPVKRGTPEYRRLEQA
                     YHRMAHLHRTNLDVLGYSHVGTTVIDHAPPLSGEGPATRVSDWGDWDAHFGPVLDGSA
                     FADLPRASVPVPALYLPFFENWPGDLRRSYKWDDPAVPRTQEEYQQIINRHALESGPI
                     EQGFSQEYQDRYAAVVAEFARHFQQKGWTKTRYLVYFNNKYYYKRPLQGGRGISWWLF
                     DEPNHRDDVLATNFLAYLTKKGLEKHPGVPIVLRTDISRVEWMRDLLSGQVDLNCISG
                     RFFEKNRYLQDDRYRFGREYWNYASTNHPRQTNVSMRAWCWRVWLNGGDGLLPWNAVR
                     GAEAWDRAEPLTVFYPGSKFGKMEPYASLRLKAYRRGQQDIEYLILLAAKHGWDRDAV
                     TNAVAGALDLSTKVSMASEEDAGSISFGKVKDADMDRVRLQIVKALLAR"
     gene            complement(12272..13249)
                     /locus_tag="VLE22_08335"
     CDS             complement(12272..13249)
                     /locus_tag="VLE22_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HSB14453.1"
                     /translation="MPLIRMLCLLLLAGLTVVSAGNPIPVILDTDIGDDIDDALALAL
                     ALQSPELDVRAVVTVLQHRERRADLCWRILELYNRTDIPVGMGAEQPLLTKPRTGPVR
                     QTEALDPEMRMPDAKRRNGIRLMIDTILSSSEKVTVVAYGPLTNVAVALKAEPRIESK
                     IERLVLMNGLFFTRIGVEYNTLRDPEAAAIVFGSGLPIVVVGLDVTMQCRLSPEDMRR
                     FDSSTLPNVQFLRRLIRIWQDGDDDRMPVLHDPLSVAVTFRPELIRPLVGRVEVETRG
                     EPGRTYGMTIFRSDANGLVQVAKEVEAPQFVSLFMERLVSAPRTSSSSR"
     gene            complement(13309..15600)
                     /locus_tag="VLE22_08340"
     CDS             complement(13309..15600)
                     /locus_tag="VLE22_08340"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14454.1"
                     /translation="MQDVTLRLLLLSIAGTLCLNASEAAAPRLDRWKVIGPGGGGSQY
                     APTVSPHNPNDVLLHCDMTGSYISHDGARTWRMFNLRGRTRFYLFDPVDPNTIYVQTV
                     GLWRSTNRGESWNLIHPNPATVTGIQMPDDHAGEQIISSGEPRGRVAALAVDPADSKI
                     LYASIPDAGAHTLYLSTDWAKTWKRIADLPGGGSQIYIDPKSPLLDRTLYVIGRNSVA
                     LRKGGKWTQGQAPSDVDAFVDTSAGFRKGGGDPVIYVAAAAGTLVSDDGGRAWRRCSL
                     PSMPFTPRVTSIAASLFHGDVAYLSYDDGRGNDRRGLGVAKTTDGGRTWEVVWDKPRS
                     TPTFVREAWMWDRNGAGWGRNFDAMGMSPTDPNLCYGSDAMRTMRSTDGGKTWEAVYA
                     VKSPDGGYTSTGVDVTTCYGVHFDPFDRNRMVISYTDIGQFRSENGGKSWSISTNGIP
                     REWRNTTYWMVFDPDVKGRVWGVMSYVHDLPRPKMWHNRSTSTYIGGICRSDDSAKSW
                     NCMPDAIPATAPTHILMDPASSPKARVLYVAAFGRGVYKSADDGKTWTLKNNGVAGTE
                     PFAWRFERDRDGVLYLVIARRSEDGSFGNDGDGAIYRSENGADHWSKVDLPKGVNGPN
                     GLAVDPRDPKRLYLAAWGRRTAIGAVDGGIFLSTDRGASWRPVFTKDQHVYDVTIDPR
                     NPDVLYACGFESSAWRSTDRGQTWRRIRGYNFKWGHRVIPDPYDPSMIYITTFGGSVW
                     HGPAEGDPNAPEDIETPAVRFSR"
     gene            15633..16574
                     /locus_tag="VLE22_08345"
     CDS             15633..16574
                     /locus_tag="VLE22_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HSB14455.1"
                     /translation="MKETAFLLFLILICGTIPLSAGEKTPIILDSDIGTDIDDAFALA
                     LALTSPEIDLRGVTTVSADAHTRALIACRMLAAVGRDDVPVAAGRRPRDIPEEKGQYR
                     YGLESPSPKRPVAELAPEFLFKQLKQNPGVLTLVAVGDLTNIARLITDHPEAKPWIKR
                     VVIMGGAVRVGYNGKPPVVWEWNIRSDIKGAQTVFTSGVPLVVAPLDATTMVKLEQPM
                     RERIFGSGTPLAAALVTLHKLWGKTTPTLFDPVAVTLSFNDSFCKMEELRLEVDNEGF
                     TREVTGKANARVATSIRTDDYLQWYVERVAGGAVKRP"
     gene            16571..18397
                     /locus_tag="VLE22_08350"
     CDS             16571..18397
                     /locus_tag="VLE22_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HSB14456.1"
                     /translation="MTTLQRFLAAAVLPAAFALPGVAQRGDLPVPADLEDHLQRRGYA
                     EKFLPAQDETLAPKPEDTRRGWLVYHRDRNYEVLPNSKPGPSETLDVLRLTATPGEIE
                     SESFSVYALRDVLDLSVSGKITGASGGSTWLASATKVEDVLFHPIQYRGEDEERTEKT
                     YLRYPMFIRPASAHPAPKGTSRLYWVTVSVPESTSAGVFRTAIEVGDGSGNRLRLPLE
                     VEVLPFTLTRKGVPAFGAFLSGQSFAKGEWAFMKRYGMDALQWFWGSHAIEIRNDNGR
                     VKMDFTKYDAFVQGMKDAGMRGPLVLSLGNSWLGHYEIKLAEAFGLRLMKRELEGRTV
                     TLMDITDPRWEKPYLEGLRLIFDHAKTAGWPPLALLINDEPTKHIMAYYPYRYHLIKK
                     HFPDIPIYGVFFQPEKDPGPLLHSCDIMVANRDLDRIRTLARDFGKRFWTYNNVTADE
                     SFGKIRLLYGQIPAYYGSEVMFFWCWNYYVGNPWDDFDGWSEKGTGPAQSDADWVAVY
                     PSVDGVEPVRTLGIEAAREAIDDVRYLKTLENVVAEREPARLEAVRGEIRRRQKAMFD
                     GIILNNRIYSDKAFFVSAKNDDVEKLRRYAVQQILETLKRSQ"
     gene            18403..20313
                     /locus_tag="VLE22_08355"
     CDS             18403..20313
                     /locus_tag="VLE22_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14457.1"
                     /translation="MRRFAILLLLAAPALAEQFSISLTGQAQPNCWNIQDIVSEVTKN
                     ARSDHEKALALHRFGMAHFIHFDGPIEERGEYMTDPLKLIGVYGFALCGNNSAAMNAL
                     YNAAGLKARRVSIPGHSIPEVWFEGRWNYIDTDMFGYVYLPDGKTMASVDDLTKDADL
                     FVRQKNPPDPFFPFDRKEDMADVFRNATPRRDYHPYASAHMMNLAVRTGESVTMFYRP
                     KDRYLLTQLREDIGAVYKDYWVVGPVRRGSLAWCDKPPAAYGNGLIEYKPDLRSEAFR
                     LENPKSEGVAVLKDREKPPLAAAAPNRPASLVVEVNSPFVIVGLQNDLTNFEDDSDAA
                     VVSGLFWRADSSDENRILVSTDAGRTWKEVWKNLWLGAVPFQVDLSRHVVGKYAYQVK
                     FDWIDRKGSGQVGLEDLQFRTWVELSPMALPRLVAGRNTFRVSTRPRSTFYNESFWQK
                     GEQLPGQHLENLDTFDQFPYLRPRDPQRPGVLTFDLGPKGSVDEARISIRARAVGGKP
                     SGTSVTLELSEDDGRTWRLLDRFTGHTEHDMNHMWFNHVIEGKTLDGEHCRLRLSVSG
                     GGFEKVIANSAVRSEPRVPTALRVTHVWREGDAQRTSEHLVRPGSGSDTYEVDAAKGL
                     VNEALRIEGVAR"
     gene            complement(20337..21260)
                     /locus_tag="VLE22_08360"
     CDS             complement(20337..21260)
                     /locus_tag="VLE22_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB14458.1"
                     /translation="MTRRQILSAGAALLGRAGLGVSTAAAQSAPTKFQIGCMTLPYAP
                     FPLERALKGIAAAGCRYVGWGTTHNESSAKRVPVMAPDAPPPAARELAKRCRDLGLEP
                     VMMFSAIYVNDPESVKVHTRRIEQAAAAGIPFIITFGHTSAEAYDVWIKNLKLLGPIA
                     RQNGVTLTIKQHGGNTATGQQCAKIIADVADDGVKLCYDAGNVLDYENNDPIPDIQTC
                     WRDIRSFAIKDHRNTPQDQDCGPGFGEIDHYKLLMPVLRTGLTIPLICENIFEPLVER
                     PKDPEGTDALVRRAREYLESVIRGLETTLKA"
     gene            complement(21263..21874)
                     /locus_tag="VLE22_08365"
     CDS             complement(21263..21874)
                     /locus_tag="VLE22_08365"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB14459.1"
                     /translation="MRAFVWVLLAAASLAQAQTGGFVSLMPKKDITEFWIVEGTPPET
                     WRVENGMIRCTGKPNGFLRSKQVYRNFVLRAEWRFQTEGWTGAPQEWPNAGFFINAGA
                     IEKGWPMSQEVQGHFGEAGSLFGVRGGKVTGAKRGPIVKDRPPFGSWDRYEITQQEGR
                     IKVVLNGKLVNEGSDAEPEEGTICLQSEGWPVDYRNVAIKELK"
     gene            21921..22730
                     /locus_tag="VLE22_08370"
     CDS             21921..22730
                     /locus_tag="VLE22_08370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB14460.1"
                     /translation="MDNKTARRHFLLAPAAAVTHPLWAQNKPLVIDAHCHAGKGTAMS
                     APWTTRADVEVTLRHMEEAGIDRTVIFPINNTEFEKPNQEVAEICGRYPKKFIGFAKH
                     DPKTESGRIAKMLRYEVEKLGLKGLKLHELPTREVLDTVAELGIPILYHPSRVSSFHM
                     LAPEYPQIPFIMAHLGSFASQNWPEHIEAIEVAKRYPNVYLDTSSVVFFKFLEMAVKE
                     AGPDKLIFGTDGPELDSRVELYKVKLLKLSPSEEAKVTGGNIVKLLPKGTV"
     gene            complement(22727..23917)
                     /locus_tag="VLE22_08375"
     CDS             complement(22727..23917)
                     /locus_tag="VLE22_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14461.1"
                     /translation="MSKKTLFLLLAVLLLSHATFAQRTLEHDYSIVSQVRIDLRELGY
                     PPVDVIPSEESAVRSLTVAPNGAIYGATSGKHSHLFVMFPVHGYVQPLCYLNGVTTVH
                     RSIVVSKSGDVYIGSGDKDGHLYRYVTKNDEHKPNRVDVACEVTDLGAPAPGEGIYSL
                     AINRMFDTIYGLTWPNGQFFSFDIVSGKFTVHGKVAERKIPGEKFETNKNIGRAMVLD
                     DAGRVFSSGENGAIYRFHPASGLEKLGITASTVRGREPYNGVDAWAKDACGMLYGGTS
                     DGYLFRLDPKTSAIENLGKPLNQYRIRGLVFARNGKLYGAGGDDDEMARLFSYDPSTG
                     AYEMLGMIDVNRRPFYTWQAYVVDSMAIGADDTIYIGQAERKSKLYLYYPEPAVTQVE
                     CEAP"
     gene            complement(23922..25151)
                     /locus_tag="VLE22_08380"
     CDS             complement(23922..25151)
                     /locus_tag="VLE22_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14462.1"
                     /translation="MAGLFAASLRVDHRVFPGLHQMDGNWAALLAASDGKVYAGLAYH
                     GGDGHLVYYDSKADRVVDVGNLTELTGESQLKRGPQSKIHSKFGEGKDGRIYFGTHAG
                     WWWNYSRFATKEGYPGAHWMAFDPKTGRVDDFGLGVPNEGVNTGNYDPRFNRIYGMTH
                     PRGHFVYYDVTKQKAVDKGRVNNWESICRTLAIDDEGYVYGSFGDGGIFKYDPKADSI
                     RELAVKVPTRPKGISLGRDYNKSETAFRTMVWDAKTQQFYGVDESATILFSFNPRAGT
                     DGEIRRLGQLAIPEMADKREVPYATLTLTIGRDRKLYYGAAGREFDYGGSAGLAAAHL
                     ITYDLNTGKTEDLGEMLLEDGSRVLGTNAADTGPDGTIYMVGAIEVRPQAGKPVEAAG
                     KIGEAYYRLALIIYRPR"
     gene            complement(25177..27276)
                     /locus_tag="VLE22_08385"
     CDS             complement(25177..27276)
                     /locus_tag="VLE22_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14463.1"
                     /translation="MKRLAVFIFLLIPCATAEAQIGNATWATPVPKDFEEPAWWNQGV
                     VFVGNWEPLVFRLRHWPELPVDVAEQYRREHTEETVIKLKEMGVNMILTHFYKTGLDS
                     DREDIEVAKQLGVLCRKHGLKLGTYIGGTIFAETLLRDVPEAKQWIRYDEHGDPVRYG
                     EQTDRYRPDFNHSGYVEHMKKVIRIAIEEVKTDLIHMDNHALIAPPWTGNSPEVNRRF
                     REFLAKKYTPAQLKDRFGFSDIRAVTVPTWHGIARPAAISPIGDPLIQEWVDFRCQDF
                     AEYYGKISDYARSLNPSVVMELNPHGIYGSNRAFLNGVDHARLVRHGSVFWSEEPNEA
                     QVDESGILVSKIRSMKLARSLDQMLFSYTGPQRANPATRSYRLLMAESMAFNRNSLGD
                     IGGPLNAYDLPDDARKYIRFYRDNNRHFASTSVVADVAVLRSFPSMATNSLAPHLETT
                     LVEQVLIQHKIPFTYVFDQDLGDLSKYRALILASQEALSDRAVEQIRDYVRRGGGLFA
                     TGHTSLYNDWRRVRGDYGLADVLKIKVSPGRALPAEGRGPFGQGRFAYLPAVTPARPI
                     QDMASTGAAGFGNSYWMLPKNAGQIASAIRYVAGGPLSVEFEGAPLTTVMELTDKTDG
                     GERILHWLNYKLGYPAGGASVTVAAPGGKKVKAVHLLSPDRDGASNVTFTAEQGRVRF
                     ALPSLEVYNVAVLEISN"
     gene            complement(27293..29479)
                     /locus_tag="VLE22_08390"
     CDS             complement(27293..29479)
                     /locus_tag="VLE22_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HSB14464.1"
                     /translation="MSTNSRRGFLKQAGVAATFPWFSPAALHGAREESYAHEMPDMLL
                     AHLSNRLNALAAKWDREREAIKTPAQLEARNSFVRQKLREMMHGFPKRAPLNPVVVAV
                     HERDGYRVENVMFQSRPDFWVTGNVYVPTTGRGPFPGIISPCGHYPWARMEPEYQFAY
                     MNMVKGGLVVLAFDPVGQGERRQYWNPQTGATEVASASTYEHSMPGQVQLLMGEDLTH
                     YRVWDGMCAVDYLLTRPEVDKGKIGCAGHSGGGTLTKFISAIDERVKCVVVNEGGATN
                     RWPSGYSPGSRLGPSDVEQNVFPAAIYGVDAHDQLVAVAPRPLLVTIEHFSPGFNRAM
                     DRIRQRYELFDVAEKFGTEEAADPHSWTPKLRIATTDWFCRWFLGKRGPEVEPRFKAE
                     APETLYCTPNGSVRYSQQGQTVFSLMLRRQAELPPNRMLPENAAGAAAFRTEMAKEIR
                     GLIHCDKPDHPLGVRNITTTPRNGYRIEKLQFLSEPGIYIPTWVFVPNAKNGRLPATL
                     YLHEEGKETDGAEFGLLEELTRAGHLVVAVDVRGIGETEPPHPPGDRYGPFAHLFDAE
                     TAMSYMAWYMDESLFGMRVRDVMRSVDYALSRQDVAPGGLRVVGKGMGALWALYAAAL
                     DTRITSVLAEGGLDSYRSLARVDRYTHSANVFIRDVLLHFDLPHVAAAMAGRNLVLIS
                     PTGPMKNPVPMASAHRTYEWTAAAYKNAGAEGRFRITDGSFGLKMP"
     gene            29552..31636
                     /locus_tag="VLE22_08395"
     CDS             29552..31636
                     /locus_tag="VLE22_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HSB14465.1"
                     /translation="MKTRLILSLVLAAWISSGQNLSDKLRALRPSDVSAGEAAVLDNL
                     ERRARESLAAIPRARTREQADAARAGLRQKLEWSLGFRRLPWPPKLAARTVGVVRRDG
                     YRIEKIIYETLPGVRVPAHMYIPDNLKAPAPAILFYNGHWWADSKTRTDFQAFHINMA
                     RLGFVVLTFDAFGQGERGISRRDHRRTEALLAGVSQQGFAVYETQCALEYLLSRNEVD
                     PKRIGMSGASGGGYNTWMTAALDERIAVAVPAVGTSEFYEQIHVCRPLDWYHASEHCH
                     FVPGLLRYANNQELIAMIAPRPLMIVAAHKDQSFPVEGVREVYRYGQELYGSYGVPGK
                     IGYSEDATTSHGYQQKKREAAYGWFLRWLMNKGDGSPFAEPPTETAPWDSEELRCFPV
                     GKNDPAGPGFVEAAKRLVRGLPPAYSAGAVQEALGPLPPLPTQQPRIEKSPLQRLKIP
                     SETGIVVPAFLLRPEGHERGVLVAAHDGGKEELAGDPLVQAALAGGWAVCGADPRGIG
                     ELAISKGGWAAAVSLLLGENFVWRQAFDLANVLAYLNASQDFAGKPAGLYGRGHGAAL
                     SVTLALAQPQGSRVKWYALRDGFTSFRQFIERPASLRESYKLISDEKKRDETYDREIP
                     YQDFAFDVLRHFDLPQVLAAAKAKGLVVNPIDGDWNRMPIEEAKKLLPRGVRTVSSET
                     PDEEIRRFLSGL"
     gene            complement(32428..34230)
                     /locus_tag="VLE22_08400"
     CDS             complement(32428..34230)
                     /locus_tag="VLE22_08400"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HSB14466.1"
                     /translation="MKHVSAPAVVCLLPLFAAAAQLPEPAVLTSEVRQHEMVEIALTP
                     DRAPGNPFDPNLVTLDAIVTLPSGQQVRVPGFWFQGYRRSIANPNATGADRVEALTPE
                     GRAGWRVRFSSGATGVHRAVLELKDPSGTSRSRDVTFAVSAGPRPGVIRVSSRNRQYL
                     EDASGRVFFPIGQNLCMYEKKEGTYYFDRLLEKLAAAGGNYVRLWQEYYVPQDPKIVA
                     GTGDGSFAGFPLETQATGLGRYDLESAWRLDHVAGVCERLNIRWQLTFEMVVWWEQSM
                     KHRWPRNPYNAANGGPCVKPLDYLTNAAARELVRRRLRYSVARWGWTTNLAAWELWNE
                     VDNMDNFDSKSCANWHREMGGYLKSIDPWRHLITTSWRDRETFAVPEIDIVQGHSYFG
                     PEYDAAEYAFQDTDHLMRGYGKPFFFGEQGIEGPVSVDPEGKHFHDCIWATAVSGAAG
                     TGMYWWWHNYIDTYNLYRHYAPLAKFVGGEDFAARKWEPARLSRPSLPVQLNVYGLMA
                     ADRALVWIHDPLAFRVIDGKAVKGAKQADASVNVVGLADGDYEIEWWDTSSGAVVRRD
                     KANVRHLRHFGYGLEMKPPEFWGDIAARVMRAER"
     gene            complement(34217..36325)
                     /locus_tag="VLE22_08405"
     CDS             complement(34217..36325)
                     /locus_tag="VLE22_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HSB14467.1"
                     /translation="MKTATLFCLACTLAGAQDFRVLDPVAARPQSLTTYLHGLADQQL
                     AQRRAEIENIKTPEQLEQRRAKVRSTLLKLIGGLPAERTPLNLKRIGRLDRGDYRVEK
                     VIYESLPGFYVTASLYIPQSGQPPYPAVIQPLGHSMNAKAGGIYQSLSIGLAKYGFVV
                     LTYDPIGQGERRIFWDGDIENSKVGGPTAEHSMVGRQSLLAGESVARYRIWDGMRGID
                     LLQSLPEVDPKRIGVSGCSGGGTLTAYIAALDDRVQAAAPACYISAWEEQLEGTGPQD
                     AEQQFPDQLRLGLNHGDFAIALAPKPYLICNTTEDFFPLDGGTRTYEELKRIYALLGA
                     GEKIARYIGPGGHGMNRDTREAIYAWMKRWLKGDPPAPASEPSFKLEAEEDLYCTRTG
                     QVADSLGGETASTLNVRRLEKIQPSRPVKDVAEKIRSLSRYQRSGAPLEIRSFGTVDQ
                     DGFRFEKVAYAADVGRIVPALLCMPAQDRDRKRAVLYVDQAGKSRASALQPDLAELAR
                     MGFTVLAVDPSGIGETAVHDEDSGPGYLADGSVWLALMVGKPVLGIRIDEILRGLDVL
                     RERNLLYDGRAIGFAKGLIGTTLLHAAALDARIDALILEGNLVSYHAVAAAPIHRNVF
                     EALIPGVLDQYDLPDLAAAVAPRPVWLVNTVSPLRNPMPSSRVEAAYRTAATQNLKVR
                     RRASAEPLMASYGELLGETR"
     gene            complement(36322..38622)
                     /locus_tag="VLE22_08410"
     CDS             complement(36322..38622)
                     /locus_tag="VLE22_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HSB14468.1"
                     /translation="MLRFSVFLALGLVVAAAAEVSPLHPRIFVRHDKAVIGTGLTVSQ
                     LRERLKNPAYARWRGTISARGAVAAVERAARYLEDGNTADLATVREFLKTNTFSYEKH
                     DVGGFLAGAEMAAAFDWVYGGLSGEDRKSAMANIVTTAESSRRFLERGGPDINHNYTY
                     MALATVAICGLALHGEPEPYGSTARDYLELSHGFLESPGMVLDTWHAREGAWAEGSHY
                     TFHETLRNLVLMLAAYRTASDRDYFAAARKDHGDFLALAGRFLIACTRPDFTFERIGD
                     CSPSRAMANITVPVTVEMLAWGLGDHPDAARLRSFDRALQEAYGEKAVHPAFGWGMRI
                     FHDPRARQTPSFRTLPLAMRMGVGTYDQIVLRNGWNEDSTQITILAGDHFTDHQHFDK
                     GHFLIYKRGGLTVDGGAYDQMYKAGGHWNEYAVRTLAHNCLLVYDPDQQLPKGHTNDG
                     GQNVLRGKQHHDDWPAYVAHREKEGLDTANVTAFDAGDGYGYVRVDLAPAYGPKVTRY
                     GREFVYLPSEDLLVVLDRVTAANGDFRKRWLLHFQERPNVDGAAPETGVTEFKGGRLT
                     AVRRQGQPNLGGRPITYDGSLFVHTLLPEQRVVSVIGGPGYEYFNSFLGVNYPTSRPA
                     VAGDARESGNWRIEVAPERPSQDDLFLHAIQMVGGSTGSPVEVRSVKALSGGLTGVQF
                     LSRPENRVVLLSGDAAGGPATLPVTYEVAADGPARHVVTGMPPGIPVEVAVNGKRLAF
                     NRVNGQGVLSFRDPGKGRRTITIRRP"
     gene            38783..40180
                     /locus_tag="VLE22_08415"
     CDS             38783..40180
                     /locus_tag="VLE22_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB14469.1"
                     /translation="MSERAADTALESLGYKPKFKRVLSLWDLILYGLVILTPTAPYPV
                     FGIVQQVSHGHAALSYLVAMVAMMFTAASYGKMAGAFPAAGSTYTYAQRALSEHVGFL
                     AGWAMILDYFLIPLLSVIYASLTAARLLPQVPYIVWALMFTALITMINVRGIQVTAQA
                     SKVMMVIMTGCAILFVWLAARWVAIEHGFGGLINQIGMYRPDTFAMGPLMLGAGIATL
                     SYIGFDAISTLAEDTVRPERDIAIATVLVCILQTFFCFLTVYLATLVWPDYKSFPEVE
                     TVILDISRTIGGPWMFGFITFVLLVAGLASALTGQAGASRLLFGMGRDGVLPQRIFAY
                     VNPKYSTPTRSIYLMAGVSLIGAGLMRFQLAVELLNFGAFVGFILVNLSVIAHYFIRK
                     RERGGMRFLTNLVFPALGALVCSYVWMNLTTKAKLVGFAWMGLGALYLAVITKGFQVA
                     PKSLGSLTEGAGDGA"
     gene            40170..41477
                     /locus_tag="VLE22_08420"
     CDS             40170..41477
                     /locus_tag="VLE22_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSB14470.1"
                     /translation="MALSPTLEERICSYVDSNRGRLVGIIRDLVRIPSENTPPVGAER
                     ACQEYIAAVLRNKGWEPLLYEPTEAPGLIEHPLYWPGRDYRNRPNLGARRKGSNGGRS
                     LVLSGHIDTVPRGTQNWTRDPFSGDVEGNLLYGRGSNDMKGGVGTNLFVVEALGELVI
                     RLAGDLVFETVVDEEFGGVNGTLAGRLMGFNADAAIISEPSFLRICAAQRGGRTAHLT
                     FRTPPNAGVLTEGEFPAGVSDQVGYFLRELRNFAEQRRKAARKHEMYDHHTDPVPVAV
                     TKISTGPWGTKEPVTIPDECKVEMYWQLMPGEVQEEVEREFFEWLDRVVASAPKLFVN
                     RPKVEFPIRWMPGSSISRSEPLVTELAACATRALGKEPPIVGIEGPCDMYVFHQAFAT
                     PAVLWGARGGNTHNADEYLEIDTAVAAAKSLLLFVCRWCGVAG"
     gene            complement(41560..42312)
                     /locus_tag="VLE22_08425"
     CDS             complement(41560..42312)
                     /locus_tag="VLE22_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14471.1"
                     /translation="METRLVRQIRALQLCVLVLFLSTAFLCANFFYPLLPQQRFRVID
                     AERINIREKSGTLKAALSNSAGFNVGQRAQMGGVRFAGLMFYNEEGEETGGLVYYGRT
                     TPGGQDSDVTLTMDQFRQDQNVYLHHEEFKDQQGLRIEDGLSINARPDWTSIKEEYAI
                     YGELQKLPPEQREELQLKSLQAGKISSNRLFFGVRRGIKNSKSYDDTGVFIRNKWGRN
                     AIKLYVDNDNKPHFEIYDSLGKSIVYELKLPQ"
     gene            42572..42724
                     /locus_tag="VLE22_08430"
     CDS             42572..42724
                     /locus_tag="VLE22_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14472.1"
                     /translation="MLWGARGGNTHNADEYLEIDTAVDAAKPLLLFVCRWCGGRSEGG
                     KRSSGL"
     gene            complement(42847..43056)
                     /locus_tag="VLE22_08435"
     CDS             complement(42847..43056)
                     /locus_tag="VLE22_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14473.1"
                     /translation="MVILYACVGLLLTLIFARVSRAVEVKAPKADDAFPTLTTSLSGL
                     AHPRKVVLKLSGLFAAQPTLDSSRT"
     gene            complement(43618..44589)
                     /locus_tag="VLE22_08440"
     CDS             complement(43618..44589)
                     /locus_tag="VLE22_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HSB14474.1"
                     /translation="MWLLMCLFSVIALAQAQHSEPLRLAGTIPLPQVEGRIDHLSIDV
                     KGQRLFVAALGNNTIEVIDVKNGKRLYSIPGLQEPQGLVYVPDTNRLYAANGGDGSLR
                     IFDGRSFQLLKTIPWGEDADNVRYDPRRDRIYVGYGSGALGVMDKEGDKIGVIPLDAH
                     PESFQLETTGSRIFVNLPGSRKIAVVDRETRKVIATWTTGGPQSNYPMALDEADHRMF
                     VVCRSPARMVVLDTLNGEVVETVGAVGDCDDVFYDSARKRIYAIGGEGAISVFQQLTV
                     DHYNELAEIGTAKGARTGWFSPDLRRLFVAGRRDGTRPAEIRIYEVE"
     gene            complement(44610..45587)
                     /locus_tag="VLE22_08445"
     CDS             complement(44610..45587)
                     /locus_tag="VLE22_08445"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HSB14475.1"
                     /translation="MQHRTRIALGVLCGVWLAGGSLPAQAQQIPPSYQEVLNYLGKTG
                     DYKDNVLKVNIPRNDIKVSVDGVATPTPFGFGGWVAMTKNRAGSDVMMGDLVLTQDEV
                     NPVMSALLDHGLEVTALHNHFFWEEPRMYYMHVHGMGASADLARRVKPALDLIGHVPT
                     HSQPSDPPAVPGKLDKAKIAQIVGHCGEEVGPVYKITVGRDDLKLVDMGAPVNARMGL
                     NTWAAFYGTDANTEVAGDVAMLDNEITPVLKALRSHGLDVVAIHQHMTDTQPMVFFLH
                     YWGRGPADKLASSFRAALDQLGKEHPAPHTHPVSSVRAQDAVPSLEAAP"
     gene            complement(45911..46510)
                     /locus_tag="VLE22_08450"
     CDS             complement(45911..46510)
                     /locus_tag="VLE22_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14476.1"
                     /translation="MDFSKAAKLGACLAKDHAEEMFRLLANYRDISASEAASRTDLHI
                     KTAQDFLETVTDLGIVARAEVREGKRPYYRYSLQQEKIQLELDLTSLRRAGKQDEKVS
                     ARIRERRNAAVHFSTSRDGQQLASILVGTGQGRDRKQRKINLTAAQGKFLYHLPFPTA
                     EPLRIDEIMRKAGLSDDSRVEVLSILELLEELRVLEVER"
     gene            complement(46517..47326)
                     /locus_tag="VLE22_08455"
     CDS             complement(46517..47326)
                     /locus_tag="VLE22_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14477.1"
                     /translation="MKELSKLGADRSITGLLPAICTLLLLAVVMFLFGKGAAILWAFL
                     LFVAFSALAWIAFARTRSKTYLASALYLSAVTVYFGSRAGQIPVVHGRLPALGLLIVA
                     ALVWLLFLVLTRQAKWRGRDILELAAQPVEPTSDGFTPRPKPVGKTEFSRDDVLAFAG
                     FARRNLIAMPYVELERVVFVPVTMGAEYRHLFAARRDYSRDTWVAFANDGGVSVNISE
                     KDYRAYRDELAFDRLCESLARVLTDFVELYRAGKAGRIIDKLDAMRVGVFS"
     gene            47464..47622
                     /locus_tag="VLE22_08460"
     CDS             47464..47622
                     /locus_tag="VLE22_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14478.1"
                     /translation="MPAEFLGRVQRQAEKRRKAARKREMYDHHSDPVPQPQLWLMEDF
                     LSLVKGRK"
     gene            47703..48254
                     /locus_tag="VLE22_08465"
     CDS             47703..48254
                     /locus_tag="VLE22_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14479.1"
                     /translation="MLLRLGHARLAVVAPFELLKVLSCSLEPARILRLKPGNGVVALF
                     LLTVLLPAALLAFLGFRAFRQERRLADHQVRDRLERVAESAARNLEEELREWQSATEA
                     IRPDRTRSIEELPARMRDAVPEPDSAAVVLPGQRCSRRFQKPNRYTTFFRGLGHGDLS
                     GWRFAVPPGALRLAELESSRKRP"
     gene            48478..48669
                     /locus_tag="VLE22_08470"
     CDS             48478..48669
                     /locus_tag="VLE22_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14480.1"
                     /translation="MYNKTLLNIMIWLFFLGGLFGFVTGMSKFFGGGTPAEYGVMGIG
                     GGFWLLCSAITIHIRTRLQ"
     gene            48963..49439
                     /locus_tag="VLE22_08475"
     CDS             48963..49439
                     /locus_tag="VLE22_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14481.1"
                     /translation="MVERTSRTAREIREEDAPTAEASLDSWKEIAAYLKRDVATVRRW
                     EKHEGLPVHRHHHLSRSSVYAYPSELDAWRANRRPLAESRHSTWWRPVPAFASVITLA
                     LALMMVGSDAQMGGSAQAAEPRFQRIEVPTGLDSISALSPDGSKLAFVSGGYGGEV"
     gene            49449..49655
                     /locus_tag="VLE22_08480"
     CDS             49449..49655
                     /locus_tag="VLE22_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14482.1"
                     /translation="MHGKVSPGIAGEPEQLTHGAEAMQLGLAWSGNGQWIAFNRSSAK
                     AAGRLRYIWFRQWAGPQRRSIFPT"
     gene            49975..52191
                     /locus_tag="VLE22_08485"
     CDS             49975..52191
                     /locus_tag="VLE22_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HSB14483.1"
                     /translation="MRALLFCLLTATSSFSQQVTDLWLRGYSVIPTPQTVKLESGEVV
                     LGAGWTIDPGKVNPQNIAVRTLRQDAAEFHGLNIGVKSGAGKVIRLAVTPGAVATKAD
                     PQIDKQAYRLLAGDGVIEITGNGEPGLFYGVQTLLQLMKRDAAGRLVIPKGSIEDWPA
                     MQLRFLHWDTKHHQDRMETLKRYLDWAARFKANMIAFELEDKFEYPSHPVIGAPGAFT
                     TAELQEIVNYGLERHIQVVPNVQAPAHLAYVLKHPEFAHLKADGNNYQSDLCDPRTYE
                     LIFSMYDDVIKASKGIDYFYVSTDEVYYAGINGRCTAPYNDENRSLKWVEFVKRAHEH
                     LSKQGRRMLIWMEYPLLPEHVKMLPADIIDGVIGEEEYLETENKMGMRQLGYVSMQGA
                     EYLFPNHLNRQSRDGVARGHLDGAFQSLAAGRYRRGNPIGVFGAAWGDSGLHNETFWL
                     GWSAVAQWGWNPGTARPAQHAAEFMRLYYGPRAEGMLEVYRSMQNQASSWQATWDRVV
                     SRVRAPGYGNSYGKGIGTTRRDETLSLPPLPQAGDLRIEPSFAKKYEKFLAEARGRML
                     ENEQLQTAIQAEFGLADRNHYNLEVLLGLTRFIGHHSRLLDGMDRAEKAMEQARDSHQ
                     KKEYAEAVGHLVEAFNRVDGARREGIRTFGELRAVFEKSQFPKGRAVGGRKFVHVLDD
                     TKDHWADRRPDLSYMSAPEESIGMEEWLKSLRSITEAYAKQHNVPVRGIADARLEE"
     gene            52192..>54265
                     /locus_tag="VLE22_08490"
     CDS             52192..>54265
                     /locus_tag="VLE22_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HSB14484.1"
                     /translation="MRRAGLLCVTAVLALGQEPGTLAERGYSVIPTPQRVQLAGGEVE
                     LDSFWSLDAGNSRAQGIAVRCLVGDFAEFHRVSLKRGTGNRQIRLAIVPGTIVTSLGA
                     ETDRQAYRLKIDGSSIEVTGNDGPGLFYGVQTLIQLPKRDGANRLLIPKGVIEDWPDH
                     PLRILHWDAQQHQDRVETLKRYLDWTARFKANMIAFQLEDKFEFPSHPVVGAPGAYTS
                     AQLQELVDYGLERHIQIVPLIQAPAHFSWALKHPEFAELRSDGNNYMANTCDPRAVNF
                     VFSLYDDVIRATKGVDYLFASTDEVYYAGIDPRCGKPYNPENRSLNWVDFVQKARDHL
                     AKRGRTMLIWAEYPLLPEHVKLLPPDIIDGVVGNSEYFEPEKKLGIRQLAYVSMQGVE
                     VHFPDHFSSESGEGIVPGRIEAANQVLSFGEHWRRAGTRKGNQIGVFGAAWDASGLHN
                     ETFWLGWSAVAQFGWKRGGAATDRHTADFMKLYYGPLSGGMTEVYRTMQRQARAWERT
                     WDQVPSRARGPSYGNSTGKGIGTQRRDMTLSPPAIPRMPDLQVTPHIGGKYAAFLTEA
                     RAQMPENEALKLELYAQFGLADRNRYNLEVFLSIAELVGHHWRLLTEMTGAEQDLVRA
                     YAAAQKSQHRRAVGQLVAAYNRIDRIERQRERTLHELQQVWEKGRYPRGQSVGGRSFV
                     DLNDDTKDY"
BASE COUNT        10220 a        16690 c        17041 g        10314 t
ORIGIN      
        1 gtccagcccg ttcagaaatg cgaccttcat gaccttccgc gcgtgagggt cgcggtcgag
       61 gtgctcgcga acctgcctgc cgacgtcctt ccagaccgcg tcgtattcgg gcgtgcgacc
      121 ctccttcggc aacgcgagcg gccaggcatt ggccgggttg ccgtgacgtt cgatgtcaaa
      181 ggggagcatc atgtggctca ccggcacacc gtagccggga ccccagtagc cgtgcttgtc
      241 cgtgaaggcc gaaccatcca ggtaattcgc aattctcttg tcgtactcgg tccagtcaag
      301 ctccgctttc gtgccctgca ccttcagacc tggacggtat gcgtaaacca gcggcaggaa
      361 gcgatgctgc ttggcgagtt ggtagtaggc cagctcctcc gccggcggca tgttctttat
      421 cgatccgttc cagacgtcgc ccttgaggtg gttctcctgt ggaagcgcaa agtcccaaac
      481 gtcgagcgct acattgacgc tgtcgcggcc gccatcccag gtcacttcga gcgtgccggt
      541 atacggcccc ggcggtgcgg cgctccgctc attgggcacg aagacgtcaa cccaaatggc
      601 gtgattccgc tggtacggga tgttgttgta caggtccgga atcgagaacg gaaagtcggg
      661 aagccagttc gttttccggt agggcatgag agcgtccgga taccagtcgc ggccaagcga
      721 cgtcttctcg taaccggtgg acgggcgccg gacttccgtg taccattcct tgaagatcgc
      781 gatattccgg ccttcgattc gcgcaccgcc cagttgttcc agcttcgaga acttcacatc
      841 gaaaccagcc tggggttcga caacttcgat gatcaactga aaggcggtga actcgttgcg
      901 ggcggctttc aggcgaaccg ttttcgacgc ggcatcccac gcgatgttcc cgttgcgggg
      961 attgccggtg gggtaatcct tatgaatcag gggctgggct tcgatgagat tgccagtcac
     1021 ggggcttacc cggacgtttt cactgacagc ccaaaggcgt acgcccggcg cttgcgcgag
     1081 ggcattcaca gcgagaaaga gcaggaacaa tagcgcacgc atgaaaacaa gactacatcg
     1141 aaagcggtgt acgatgtacg gacgatgaca cgcattttcc tgttgctgat cttgtcttgc
     1201 tgcctgcccg ctcaggagca gcgcaccgag agcttccttg gcaacctcgc tccgatcata
     1261 cgttcgattc atcaggaacg cggattccct atgtcgtacg cagaccggaa aggcctgcct
     1321 gtcgatcagt ggcgcagccg ggggcgggca gaggtgcagc gggcgctgtc gtactcgccg
     1381 aagaccgtac cgctcgatgt gaaagtcgag tcgaccgttg agcgggacgg ctacgagatc
     1441 cgccggatca ccttcgccgc ctcgccgcac taccgcgtgc cggcattcct gctcgttccc
     1501 aagcgaggca aggggccgta ccccggcgtc gtggcgctgc acgaccatgg cgggtggttt
     1561 ttccacggca aagagaaact cgtccgcatg gagggagagc accagtcgct gaaggacttt
     1621 cgcgagcgct actacggagg acgaacgttc gccgatgaac tggcgaagcg cggtttcgtt
     1681 gtgatcgtgc cggatgcgtt ctattggggc gagcgaaggc tccagtacgc tcaaccgccg
     1741 gcggattttc agaaaaggct cgccgggctc gatccagcgt caaccgaata cgtgctcgcc
     1801 gtgaactcct atttgcgaga gcggaacacg gaactcaaca cgtggctgtc gttttgcgga
     1861 acaagctgga tgggcatcgt ggttcatgac gaccgccgta gtgtggatgt gctggcgtcg
     1921 ctgccggagg tggacgcgaa acgcattggc tgcctggggc tgtcgggcgg cgggtaccgt
     1981 tccacctatc tcaccggcat ggagcctcgc attagggctt ccgtgatcgt cggttggatg
     2041 acgtcgctgc cgacaacgct cgacatcaca tactcagtcc actcggcgct gttcgacgct
     2101 cacggagcgc atgccaatct tgatcacccc gacatcgctt ctctggctgc gcccgattgc
     2161 gcgacgttcg tgcagaactg cgcgcgcgac cgtctcttta cgcgagaggg catggagtcg
     2221 tcggcggaga agatccgggc cgtgtactcg gacctcaaac accccgagcg gtaccaggcg
     2281 aggttctacg atgtcccgca ccagttcaac gtccagatgc aggaggatgc cttcgcctgg
     2341 ctcgagaagt ggctgggcgg acgctaagac gcccgtacag gttccctgtc agacttcgag
     2401 cttccatggc gcgcggtacg gccggaggag gtactggagc gcttcctgtt ggagcacggt
     2461 ctgcttctgg tcgtcccaat cgaggcgaag tttggagcga tacgaggcgt tgccgagcag
     2521 acaggtcgct gaagaccgat agcatttctc gatgtcgctg ttcgggcgct gacgcgtttt
     2581 cacgcactcc agaaagttga cccagtggag cgggtgcggg tcggccaccc gtttcatttc
     2641 gaaggcctca aggtcagagc ccttttccgg cgtcacgcgg cacacgttgc ggtcgacgta
     2701 gagcgtgccc cgcgtgccgt ggaagttaac gcccatcaga cggctggtca ttccttcggt
     2761 gttgttgcag cggtgctccc aggagccaat gaatcccggg tattcgaagg acaccagcat
     2821 cgtgtcgggc gtctcgcggt tgtcggtgaa ccagagcttc ccgcccaggg cggtaatggc
     2881 tttcggcatc gggtcgtcta acgccatttg cagaatgtcg agcatgtgga cgccggaatc
     2941 ggttagctgg ccgcccgcgt aatcccagaa ccagcggaaa aacgagtagc tgtgcgggta
     3001 gacgccgaag cgattggggt tgaaggggcg gtccggcgcc ggcccgagcc acagttggta
     3061 atcgaggtcc ttcggtggcg tcccatccgg tgggttacca atgcctgcct gcggcgcgtt
     3121 ctcgtagatc cagctccggc agaacgtgat cttccccaat tggccgctgc ggacgatctc
     3181 agcggccttc tggaagtggg caccggagcg ctgccaggtg ccggcctgca cgacgcgctc
     3241 gtacttacgc gcagccgcga ccatcttctg gccttcgtcg ataccgagac aaaccggttt
     3301 ctcgacgtag acgtccttgc cagccttgca ggcttcgacc tccatgtacg gatgccagtg
     3361 gtcgggcgtc gagatgcaga cgaagtcaat cgacttgtcc gcgataatct cgcgaaaatc
     3421 gcggatctct ttcggctgat ggttctggcg gcgggcaatt gcggccgccc gctccatgtt
     3481 gggctggaat acgtcgcaga cggcggcgat ctccacgccc ggctggcggg cggcgacgcc
     3541 gaggttctcc gagcccatca cgccggtgcc gatgaaccct ccgcgaatcc tgtcgttcgc
     3601 tccgcggatg ctgccggtga agatcgatgt cgtgaatgcg gcccctgcgg cctttagaaa
     3661 ttgcctgcgg gcactgtcag atgtgtccat ttgagtcctc cgtaaaatga gctttcagtt
     3721 tacccgccgg gctgcgggcc gtgcaggatt gcgcggcacc gccaattcga gccggtgcaa
     3781 atgccgcgag tgcaactgga atgcctggta aagcttttcc gtgccgtagc gtacgtcggg
     3841 cgtgtagtga tagctccacg attcgttgtc gagcaacagg aggatcttcg ccccggcggg
     3901 cgcatcgggg gaaaggcgga tcaggctggt cctttctttc gagctggtcc actcaagctg
     3961 cttctgttcc tggatgtcgc cgatttccgt gacatggggc gaatcggtgt agactttcgt
     4021 acggtaccag ttgttcttgt cgaacgggtc cataccatag gccagcttga cccagatagt
     4081 cgcctcctcg cccggttcga ggattccgtt cccgttgccc ttgccttcgg ttaccgtgcg
     4141 ggaaatcgat ccaccgccac cctggttgcc cttctggcgg aagacgttca acgtcatcgt
     4201 gcggccgtcg agaatctcga ccgccagtgg cacggggatg gcttccggga tgatcaggac
     4261 gtccacgttc ttcgacgtct cgtgccagcc gtcgaacgtc gttgtgactg taaggcgggc
     4321 cggcgcaaag tggccggtac cggcggtgaa cgtggcgcga agccggtcgg acaggtcgac
     4381 gaagtcgccc ggttcgatct tcgggaccgc ggccgtctcc gccaccagcc gcacggtcgg
     4441 atactcgctc gacaccgcga ccttcacatc ggtcatcgcc tcgccgcggg gattgtagat
     4501 gcggatgggc atccggactt cggtgccggg cggcagacga agccggtctt tcggagtaac
     4561 aggcaacaga accggcggtt cggcgcccgt acccggtcca gagaagctga cttggtgccc
     4621 ggctccgtcg acggtgaaac tcatccggcc ggcggtgtcg ggagttactt cacgggtgtt
     4681 ggtcttgccg gcggaaagat cgtggtcgaa cagtttgtac gcgcgccctg cctgatagag
     4741 cggaggggtg gtgatcgtga tccgccggtc tcgaacgggt ggcccgtccg gttcccagcg
     4801 gcgcgtggtc acccgtagac cctcctgcct gacgccctcc agcaaggtga tgcccggctc
     4861 gtttccttcg gcctcgacgc gatagcccca gacctcgaac ttacggtacg ggtttgcgtg
     4921 actgaagact tccggcacat tatccagcgc ggatttagcg aaggcccgca tgtggaagtc
     4981 gaaggtctcg cctatggagg tcgcccagtg gcgatggtac tcgtcctgac ggtactcgaa
     5041 atccacctcg tgcgcgcgag cgtacacgtc gcgcatctcc tcgtggtact ggctgatgta
     5101 gtcgccgctg gcgcgaatga gccgtaccat cgtgtgccca tggttcgcgg catggtcctt
     5161 caaacgccaa agcgtgcggc ggcctttgtc accgacgtag aactcgtggc cggggttgaa
     5221 cgccgaggcg ctcccgatga ggtgcggata ccgtgcgctc gtccacaggc tcatgaagcc
     5281 gcccatgctg aggccggagg tcgcgcggtg gcgcctgtcc gtcagcgtcc ggtaggtaga
     5341 gtcgatgtgg gcgacgagtt cctgaaagta ttcgccgaag tcattatcgc cgccgttctc
     5401 cagaagatcc catggcgagc cgccgtagaa gccggtgtaa ttcttcgcga cgaatccatc
     5461 cacggcgacg acaatcacgt catgtttcgc gacgaaatcg gttattttgg gcaccgtgtc
     5521 cgtgccgttg tcgtaccgct ccagcgtgta gcggtcgctg tgtccatgga aatagtagat
     5581 gacgggatag gccttcccac tcgtctcgta cgcgggcggc agaaagatgc ggtaatagcg
     5641 cggctgcccg aaaaccttcg agggatgagt gcggtcgtgg aacggctgcc gcgcggaacc
     5701 ggcggccgcc gcaagagcga gtagaattgt caggacgtat cgcatcatcg agaatcatag
     5761 aggatttggt gactgcatga tcaggatcgg aatcattctt gtgtcctttc tgctctgcgg
     5821 aagcgtggga gcggcggacc tgtgggagct ggcgaacagt aagcgcgacg tgcaccggtt
     5881 ctcaactctt ttcacggcgc aggacgtgcg ggaccgcctc tcgaccgggg acagcatcga
     5941 cgccgccatc gattggtgca agcgtactgc tgtgacgcgc gtctacatcg aatcgttccg
     6001 cgacggctac caggccgagc ggcagacgct gctgcacgcc aaggagcgct ttcagaaggc
     6061 aggaatcgac gtctcagggt gcatcactac gaccggtgtc ggcaagcgct cgaccggctg
     6121 gcggccgttg tcctgctaca ccgacaagcc cacccagcag aagatcaagt cggtcttcga
     6181 gttcaccgcg tcgatcttcg acgagatcat gatcgacgac ttctggttca ccgactgcac
     6241 gtgcgccgag tgcgaagcag cccggaaggc gcggacggcg acagtcggtg acaaggccta
     6301 cccggtcccc ggcgacacat gggaagacta ccgctgcgag ctgatggtcc gcctctcgcg
     6361 cgactacatg ctggcagccg caaagaaggt caacccgaag gcgcgactca tcatcaagta
     6421 cccgcaatgg tacgaccgct ttcacgaccg cgggtacgag gttgcgcggc agacggcgga
     6481 cttcgaccgt atctgggtag gcaccgagac gcgcgatcgg gacaagggcg gcgtcgtgcc
     6541 ctacgaagcg tatttcatca tgcgatggct gggacggctc ggcggcagca agaccggcgg
     6601 cggctggtac gatccgtttg acacgacggg acagacctat atcgagcagg cgcgccagac
     6661 cgtgctggga ggcgcgcgcg aatcgatgct gttctgttac ggcggcctgc tcaggaacac
     6721 cgggccggcg aacgttgagg agcttcgcgg gcacatcccg gaattgttcg tgatcgccga
     6781 cgaggtaggc aaacggcagc ccgctggcat cgccgcgtac aagccaccga acagccatcc
     6841 ggagaaggaa cagttcatct tcgattacat cgggatgctt ggcttgccgc tcgttccttg
     6901 ccacgagttc ccggaggacg cgcaagcggc gtttttctcc gtccacgcgc taaaggaccc
     6961 ggacttcgcc gagaagctgt cacggctgat cggacgtggc aggccggttt tactcactga
     7021 cggcctggtg caggggctgg gaggaaaggt gaagctggac gtcgaaaacg tgcagatcct
     7081 gccggtcggg ggcgacccga agtcgctgct gaaactgtca cgcgaaaagc tggacgtgat
     7141 gcgagagccg ctcctgcggc cgttccgggt ggctttcagc gcgcccaacg aggccggctt
     7201 gtacctgttc gacgacggga gctgggtggt cgagaacttc accggccagg aggcgagtgt
     7261 cgatctcaac gaaaagaagc ttaagatcgc cccccgagag tggacctacg agtggcgggc
     7321 gccgtcgccg cgcgacaccg tcgttatccg gcccgtcgag attcacgatg tgctggtgaa
     7381 ccccggcatg ggcattcaga cgttccagcg gttcaacgga gacccgccgc tccgggacat
     7441 gacgtggtcc gaggaaggcc cgacccgacc gtcgccggtg cccaccgcga aaccggacta
     7501 ccccggttct tcaatcgcct attttcgctg gtattggata caaatcgagc cgaaagaggg
     7561 ccaattccgc tgggaaatcg tcgacaacgc gatcgccgaa gcgcagaagc gcggccagaa
     7621 actggacttc cgcgtcatgc cctacgacga gggacacccg ctaccggagt ggtaccgcaa
     7681 caccggcgcc cggcgcgcga acaagccgac ggacccggac ggccagatct ggtcccccga
     7741 ctcgggcgat ccattgtact tgaagcactg gaacaggctg acgcgcgaac tcgggaaacg
     7801 ctatgacggc caccccggtg tgaatagtgt ggatatctcc actgttggct attggggcga
     7861 aggatgggga ccctatctgc ccgattggaa cgcgcagcag gcgttgatcg acgcctatct
     7921 ccagtcgttt cggaagaccc cgcttctgat gaactttgac gtgaccgagg ccttggctca
     7981 cgcgacgaag aacggtgcgg gctggcggct cgattgttgg ggcgacatgg gcaactactg
     8041 ggagagttgg ggcacgtacc tctcgcacat gttcgacacg tatccacaga cgctcgtgcg
     8101 ggccggagcg caggaggcat ggcggagcgg tccggtctcg ctcgaaacct gttgggtccc
     8161 gccatactgg aaagcccaca actacaacat cgactacatc atggagcagg ccctccgctg
     8221 gcacgtaacg tccgtgaaca tcaagtcggc gcgcatccca ccggagtgga agggaaagtt
     8281 cgaggagttc cagaagaaga tgggctaccg tttcatcctg cggcggctgg agtacccgaa
     8341 gcgagtgaag gccggcgcga agatgcccgt gaacatgtgg tggctgaacg cgggcgtcgc
     8401 gccggcatac gatccctacg acctggcgct ggaattacgc tcgggaaata caggatcggt
     8461 cattcggctc ccggcggacg ttcgcaaatg gatgccgggc gactcgctcg ttgaaggcga
     8521 cgtgtccttc ccggccagtc tgaagccggg ggaatatcgc gtgcgagtag ggctcctcga
     8581 tccgcggacc ggaaacccag cggtgaagct ggccatcgag ggcaggcagc ccgacggctg
     8641 gtacgacgtc ggaagcatcc gggtggagtg agggcggctc agcgcggatc cacgtactcg
     8701 acccaaacga gctcgcacga accgcttccg ctgtcggtgc ggcttaaaga actggtccag
     8761 ctccagccgt ccttaccgga gacgcggacg aggtagcctc ccaagccgac gagctgcccg
     8821 ttgcgcagcg ccagcaactg gtccttgatc tggtccgtgg atgggatcat gtgcatgttc
     8881 gagctgtggt tggcaatctc ggtcatcggg attggcggct ggttatcctt gggcatccag
     8941 cgaaaccagc gctggccctg ccagatcgat agccggtcga tcacccgctg atccgacatc
     9001 ggtccccagc ccagcgcgag atcgaccggc gcgacttttg ctccgccgtc gaaccagtag
     9061 cgctcgctgc gaagcacgcg ggcacgcagg cgaaagctag ccagaggctt gatggtcaag
     9121 cccttgtatt cccagggcgc gacggattcg gccggactct gagacgggtc ttcggaaacg
     9181 aggacacccg gcggatacgt gacgctaccc tcatagagcg catacaccac agcgatgcct
     9241 accaggattg caccaaccag cagcctcctc gaggaattcg ccgcggcgtc gatgatccgg
     9301 accgacatgt caacgcgcga gcagggcctt cactatctga aggcggaccc ggtccatgtc
     9361 ggcgtctttc accttgccaa agctgatgct gccggcatct tcctccgacg ccatgctgac
     9421 cttggttgag aggtccagag ctcctgcgac tgcgttcgtg acggcatcgc ggtcccatcc
     9481 gtgcttggct gcgagcagga tgaggtactc aatgtcctgt tgtccccggc ggtatgcctt
     9541 gaggcgcagc gaagcgtagg gctccatttt tccgaacttg ctgcccggat agaacacggt
     9601 cagcggctcc gcgcggtccc aggcttccgc accacgcacg gcgttccagg gcagcaggcc
     9661 gtcgccgccg ttcagccaga cgcgccagca ccacgcccgc atactcacgt tggtctgcct
     9721 gggatggttt gtggatgcat agttccaata ctcgcggccg aaccggtagc ggtcgtcctg
     9781 aaggtagcgg ttcttttcga agaatctgcc gctgatgcag ttcagatcaa cttgcccgga
     9841 aagcaaatcg cgcatccatt cgacgcggct gatgtcggtg cgcagcacga tcgggacgcc
     9901 gggatgcttt tcgagtcctt tcttcgtcag gtaggctaaa aagttggtgg ccaggacgtc
     9961 gtcgcggtgg ttcggctcgt cgaacagcca ccaggagatg ccgcggccgc cctgcaacgg
    10021 gcgcttgtag tagtacttgt tgttgaagta gacgaggtag cgggtcttcg tccatccctt
    10081 ttgctgaaaa tgccgggcga actcggccac cacggcggcg tagcggtcct gatactcctg
    10141 actgaaaccc tgctcgatcg gccccgattc gagcgcgtgg cgattgatga tctgctggta
    10201 ctcctcctgg gtgcgaggca ccgccgggtc gtcccacttg tagctccggc gcaggtcgcc
    10261 cggccagttc tcaaaaaacg ggaggtagag cgccgggacc ggcaccgacg cccgcggcag
    10321 gtcggcgaaa gccgagccgt cgagcaccgg tccgaaatgc gcgtcccaat cgccccaatc
    10381 cgacacccgc gtggcagggc cctcgcccga caggggcggg gcatggtcga ttaccgtggt
    10441 gccgacgtgc gagtaaccga gcacgtccag gttcgtgcgg tgcaggtggg ccattcggtg
    10501 ataggcctgc tccagcctgc ggtattcggg cgtcccgcgc ttgaccgggt agccggaatg
    10561 gacgctgcta tagcagttga ggtccacgat gaagttcaac ttgtccggca gggtcaacgg
    10621 caggacttcc acctggaggg cgacgcgccg ttcggttccg cccgcactga ccagcaactc
    10681 accgcgatgg acgcccggct tcgcgtcgtg cgggatatag acgtcgacga aaatgggctg
    10741 aaccgtctgc tcaggcaccg ggttgtcctt cgacggcaca tcgaacggac cagcgagcgg
    10801 caccagagcg tcggtgtacc actcccggat tccgcgcttg ggctggtccg ctacggccca
    10861 ctcgcggtaa agctgtatgg cgccggtttc cttgaatatc gctggcaaag tgctctcgct
    10921 gaacaggcgc ttggtcacgc gcgcctcgat tcccgcgagc ggctgcttgc tctcgacggc
    10981 gagatggaaa ccgaggaact cgttccgagc gcccgtcagc ctcaccgtgg cggatgaggc
    11041 attccagatg ctgttgcgcg agcggtggcc ggcgaagtcg tctttttcga tcaattcgcc
    11101 cgtgcgcggg tcgatcttca gcgtctcggg gaccgcccag acggagaagg cgtctgccgc
    11161 cggcggctgg aaagcggctc cggccgtctc gtcgccggga ggtagcttcg ggaattcagg
    11221 ggagtacgcc cggtagcttc ccagagccga gaccggtcca gcgttgcctg cctcgtcgat
    11281 ggcacgagcg gcaaagtagt acacctgtcc aggcttgagt tgctcgacca ccgcgttcac
    11341 ctcgtcacgc agggaattcg aggtcgcgaa cggatttggc ttcggcgcca gcgggtcgag
    11401 catccagcgt ggcgctcggg acgcggagtc gaaactcaca gtggcgatcg gtgtctcgct
    11461 gtatcgcact tcgtaaccag tggcgacgcc ctgtccggcg tcgtcgccgg cgccgccgaa
    11521 gtgcagaatg acactgcccg ggcgcagcgt cgagcgagac agttttctcg ccgccgcagg
    11581 gctgctgtcg atcacggtcc cggttctgaa agagcgcacc gtgccgggcg ccgtccggtc
    11641 ccgccgcgaa ccctccacaa tcaggaccgg aggatttccg ctctcccggc tgctgatttc
    11701 gtgacgggtc tgtgtctggc ccttttcgtc ggtcaacatc aacccgtatt gatcgccgcc
    11761 gatcagagcg tgaacgagcg cgggtgggac gctggcctcc caccaatcgc ctcccgcgtc
    11821 ccgtgcacgt tcgtaagagt acaggctgcc gccctgcgcg aacgtcacgt caacaaggtc
    11881 ggatgcgggg taggaccatg cctgcttctc gggacccgga aagaagtagt tgaccgtggg
    11941 ctcggaccaa ggcccgttgc cgctgatcgt ggacaggccc accgtatgga gcggtaccgg
    12001 agccggtttg cggtggatcc gcaacgtggc tttctccacg gtgaggccac gagcagccga
    12061 aacatcgaac tggagcagcg cgaacgccgt ccggccctga ataacgagag cggcgtcagc
    12121 gccgtggttc tcgagcgcgt cattccgatc ggactttggt ccctcccagc ggtgcatcga
    12181 gacccacgta tcggcggcca cgggacattc aatcctctct gcagcacaag ggaggcagac
    12241 aagcagcaag aaaaagaggt tcttcatttg atcaccggct cgaggatgat gtgcgcggag
    12301 cactgaccag ccgctccatg aacaggctga cgaactgcgg ggcttctact tccttggcta
    12361 cctggaccag gccattcgca tccgagcgaa agatggtcat tccgtacgtt cgtccgggct
    12421 cgccgcgtgt ttcgacctca acgcgtccca cgagcggccg gatcagctcg ggacggaagg
    12481 tcacggccac ggagagcgga tcgtgaagaa ccggcatccg gtcgtcgtcc ccgtcctgcc
    12541 agatgcggat caaccggcgg aggaactgga cgttcggcag ggtagaggaa tcgaaccgcc
    12601 tcatatcttc cggcgagagg cggcactgca tggtgacgtc cagtccgacg acgacgattg
    12661 ggaggcccga accaaagacg atcgcggccg cctccgggtc gcgcaaggtg ttgtactcga
    12721 cgccgatccg ggtgaagaac aggccgttca tcagcaccag gcgctcgatc ttgctctcga
    12781 tgcgcggctc ggccttgagc gccacggcga cgttggtcag cgggccatag gcgaccaccg
    12841 tgactttttc ggaagacgac agaatcgtgt caatcatcag ccggatgccg ttgcggcgtt
    12901 ttgcgtcggg cattcgcatt tcggggtcca gcgcttcggt ctggcggacc gggccggtcc
    12961 tcggcttggt gagcagtggc tgctcagcgc ccatgccgac cgggatgtcg gtacggttgt
    13021 acaactcgag aatccgccag cagaggtcgg cccgccgctc ccgatgttgg agcactgtaa
    13081 cgaccgcacg cacgtccagt tccggtgact gaagagcaag ggccagcgcc agtgcatcgt
    13141 cgatgtcatc gccgatgtcg gtatcgagaa tgaccggaat cggattgcct gcggagacga
    13201 ccgtcagccc agccagtaga agaaggcaca gcatgcgaat cagaggcatc gcgttgcatg
    13261 atagtgcctc gtctgcggct ttttcaacct ttgctccggc acactacctc agcgggagaa
    13321 ccggaccgcc ggagtctcaa tgtcttccgg cgcgttcgga tcgccttccg ccggcccgtg
    13381 ccagacgctt ccgccgaacg tcgtgatgta gatcatcgac gggtcgtagg ggtcagggat
    13441 cacgcggtgg ccccacttga agttgtagcc cctgatccgc cgccacgtct gacctcgatc
    13501 cgtcgaccgc catgctgacg attcgaagcc acaggcatac aggacgtcag ggtttctcgg
    13561 gtcgatggtg acgtcgtaga cgtgctggtc tttcgtgaag accgggcgcc acgacgctcc
    13621 ccggtccgtg gagaggaaga tgccgccgtc caccgctccg atggctgtgc gccggcccca
    13681 cgcggccaga tacagccgct tcgggtcgcg cggatcgacg gccagaccgt tcggaccgtt
    13741 gacgcccttc gggagatcta ctttcgacca gtgatcggcg ccgttttcag aacggtagat
    13801 cgcaccgtcg ccgtcgttgc cgaagctgcc gtcctcgctg cgccgagcga tgacaaggta
    13861 gagaacgcca tccctgtctc gctcaaatct ccaggcgaac ggctccgtac cggcgacgcc
    13921 gttgttcttc agcgtccagg tcttgccgtc gtccgccgat ttgtagacgc cgcggccgaa
    13981 ggcggcgaca tacagcacgc gagccttagg tgaactcgcc gggtccatca ggatgtgagt
    14041 gggcgctgtc gccgggatcg cgtccggcat gcagttccag ctcttcgcgc tgtcgtcgct
    14101 gcggcaaatc ccgccgatat acgtcgatgt agaccggttg tgccacatct ttggccgggg
    14161 caggtcgtgg acgtagctca tcacgcccca gacgcggccc ttgacgtccg ggtcgaagac
    14221 catccagtac gtcgtatttc gccactcgcg cgggatgccg ttcgtcgaga tcgaccaact
    14281 cttgcctccg ttctcgctgc ggaactggcc gatgtcggtg taggagatca ccatccgatt
    14341 tctgtcgaac ggatcgaagt ggactccgta acaagtggta acgtcgacgc cggtcgaggt
    14401 atatccgccg tcgggagact ttacagcgta gacggcctcc cacgtctttc caccgtcggt
    14461 ggagcgcatg gtgcgcatgg cgtcggagcc gtagcagaga ttcggatccg tgggggacat
    14521 gcccatcgcg tcgaagtttc tgccccatcc cgcgccgttt cgatcccaca tccacgcctc
    14581 ccgtacgaac gtgggagtgg agcgaggttt gtcccaaacg acttcccacg tccggccgcc
    14641 gtcggttgtc ttagccacgc ccagaccccg gcggtcgttg ccccgcccgt cgtcatagga
    14701 gaggtaagcc acgtcgccat gaaacaggct cgcggcgatg gaagtcaccc gcggcgtaaa
    14761 cggcatcgaa ggcagcgagc agcggcgcca cgcgcggcct ccgtcgtcgg agacgagggt
    14821 ccccgcagcg gcagcaacat agatcacggg atcgccgcca ccctttcgga aaccggccga
    14881 agtatcaacg aacgcatcca cgtcggaagg cgcctgtcct tgcgtccact tgccaccctt
    14941 ccgcagtgca acggaattcc ggccgatcac gtatagggtc ctgtccaaga gtggtgattt
    15001 cggatcgata tagatctggc tgccgccgcc ggggagatcc gcgatccgct tccaggtctt
    15061 agcccaatcg gtggacaggt acagagtgtg tgcacccgcg tccgggatcg aggcgtacag
    15121 gatcttcgaa tcggcgggat cgacagcgag cgccgccacc ctcccacgcg gctctcctga
    15181 ggagatgatc tgctctccgg cgtggtcatc cggcatttga attccggtga ccgtagccgg
    15241 attcgggtga atcaggttcc agctctcgcc acggttcgtg ctgcgccaga ggcccacggt
    15301 ctgcacgtag atcgtgttcg gatcgaccgg atcgaagaga tagaagcggg tgcggccgcg
    15361 gagattgaac atccgccagg tccgtgcgcc gtcatgcgag atgtaggaac cggtcatgtc
    15421 gcaatggagg agaacatcgt tcgggttgtg cgggcttacg gtcggggcat actgagagcc
    15481 gccgccgccc gggccgatca ctttccatcg gtcgagacga ggcgcggccg cctcggaggc
    15541 attcaggcac agcgtgcccg cgatcgagag cagaagcagg cgaagagtca catcctgcat
    15601 gataccctcg tgatggtgca ccctcaaccg ttatgaaaga gactgctttc ctgctgtttc
    15661 tgattctgat ttgcggcact atcccactat cggccggcga gaagaccccg atcattctcg
    15721 acagcgatat cggtaccgac atcgacgacg ccttcgctct cgcgctcgcc ctgaccagtc
    15781 ccgaaatcga cctccgcgga gttacgacgg tcagcgccga cgctcacacg cgtgcgctga
    15841 tcgcctgccg gatgctcgcc gccgtgggac gagacgacgt gcccgtcgcg gcgggccgcc
    15901 gaccgcggga cataccggaa gagaaggggc agtaccgcta cggtctcgag tcgccgtcgc
    15961 caaagaggcc tgttgcggag ttggcgccgg agtttctgtt caaacagctg aaacagaatc
    16021 ccggtgtgct gaccctggtc gcggtcggcg atctcaccaa catcgcccgt ctgatcaccg
    16081 accaccccga agcgaagccg tggatcaagc gcgtcgtcat tatgggcggc gcggtgcggg
    16141 ttggctacaa cgggaaaccg ccggtagtct gggagtggaa catacgatcc gacatcaagg
    16201 gggcgcaaac cgtcttcacg tccggcgtgc cgctggtggt ggctcctttg gacgcaacca
    16261 cgatggtgaa gctcgaacag ccaatgcgcg agcgcatctt cggttccggg acgccgctcg
    16321 ccgccgcgct agtcacgctc cacaagctgt ggggcaagac aacgcccacg ctcttcgatc
    16381 cagtcgcggt gacgctgagc ttcaacgaca gcttctgcaa aatggaagag ctccggttgg
    16441 aggtcgataa cgagggcttt acccgcgagg tcaccgggaa ggcgaatgct cgcgtggcga
    16501 cgtcgattcg cacggatgac tacttgcagt ggtacgtgga acgcgtcgcc ggcggcgcgg
    16561 tgaaacggcc gtgacgaccc tacaaagatt ccttgcggcg gccgtcttgc cggcggcctt
    16621 cgctcttccc ggtgtcgctc agcgcgggga tctgccggtt cccgcggatc tcgaggacca
    16681 cttgcagcgg cgcggatacg cggagaagtt tctgcccgcg caggacgaga cacttgcgcc
    16741 gaaacctgag gacacacggc gcggctggct ggtttaccat cgggaccgaa actacgaggt
    16801 gctgccgaat tcgaagcccg ggccatcgga gacactcgac gttctccggc tcacggcgac
    16861 gccgggggag attgagtcgg aatcgttttc ggtgtatgcg ctgcgcgatg tgctcgactt
    16921 gtcggtctcc ggaaagatca ctggcgcatc tggaggaagc acctggctcg cttccgccac
    16981 gaaggttgag gatgttctct ttcacccgat ccaataccgc ggcgaagacg aggagcgcac
    17041 cgagaagacc tatctgcggt acccgatgtt cattcggccg gcgtcagcac acccagcgcc
    17101 gaaaggcacg agccgactgt actgggtcac ggtgagcgtg ccagaatcga cgagcgcagg
    17161 tgtgttcagg acggcgattg aggtcggcga cggctcggga aaccggcttc gactgccgct
    17221 cgaagtcgag gtgctcccct tcacactgac ccggaagggc gtgcccgcgt tcggcgcgtt
    17281 cctcagcggg cagtcattcg ccaagggcga gtgggcgttc atgaagcgct atggcatgga
    17341 cgcgctgcaa tggttctggg gctcccacgc gatcgagatc cggaacgata acggtcgcgt
    17401 aaagatggat ttcacgaagt acgacgcctt cgttcagggc atgaaagacg ccgggatgcg
    17461 ggggccgctc gtgctttcgc tcggaaatag ctggctgggc cattacgaaa tcaaactagc
    17521 agaggcattc ggactgcggc tgatgaaacg ggaactggag ggccggacgg tcactttgat
    17581 ggatataacc gaccctcggt gggagaaacc gtacctcgaa gggctaaggc tgatcttcga
    17641 tcacgccaag acagcggggt ggcctccact cgcgttgctg atcaacgacg agccgaccaa
    17701 gcacatcatg gcgtactacc cctaccgcta ccacctcatc aagaaacact tccccgacat
    17761 accgatctac ggcgtgttct tccagccaga gaaggacccg ggaccgctac tccacagttg
    17821 cgacatcatg gtagccaacc gcgacctgga tcgcatccgg acgctcgccc gcgattttgg
    17881 aaagagattc tggacgtaca acaacgtcac ggcggacgag tcgttcggca aaatccgcct
    17941 gctctacggc cagatcccgg cgtactacgg gagtgaggtg atgttcttct ggtgctggaa
    18001 ctattatgtc ggcaacccgt gggacgactt cgacggctgg agcgaaaaag gcacgggtcc
    18061 cgcgcagtca gacgcggact gggtggcggt ctacccctcg gtggacggcg tggaacccgt
    18121 tcggaccctc ggcattgagg cggcccgcga agcgatcgac gacgtgcgct atctgaagac
    18181 gctcgagaac gtcgtcgccg agcgcgagcc ggctcggctt gaggcggtcc ggggcgagat
    18241 ccgccgccgg cagaaggcga tgttcgacgg gatcatcctc aataaccgaa tttactccga
    18301 caaggcgttc tttgtgtccg cgaaaaatga cgacgtggag aaactgcgcc gctatgccgt
    18361 tcagcagatt ctcgagactt tgaaaaggag ccagtgagct tcatgcgacg tttcgcaata
    18421 cttctcctcc tcgccgcgcc ggcgctggcc gaacagtttt cgatcagcct gaccggccag
    18481 gcacaaccga actgttggaa cattcaagac atcgtctccg aggtcaccaa gaacgccaga
    18541 agcgaccacg agaaagcgct ggccctgcac cgcttcggca tggcgcattt cattcacttt
    18601 gacgggccga ttgaagaacg cggcgagtac atgaccgatc cgctaaagct gatcggtgtc
    18661 tatggcttcg cgctctgcgg caacaattcc gccgccatga acgcgcttta caacgcggca
    18721 gggctgaagg cgcgccgggt ctcgattccc gggcactcca tcccggaggt ctggttcgag
    18781 ggccgctgga attacatcga caccgacatg ttcggctacg tctacctgcc cgacggtaag
    18841 acgatggcct cggtcgacga cctcaccaag gacgcggatc tcttcgtgag gcagaagaat
    18901 ccgcccgatc cattcttccc gttcgaccgg aaagaagaca tggcggacgt ctttcggaat
    18961 gccacgccac gccgagacta ccatccttat gcgagcgccc acatgatgaa cctggcggtc
    19021 cgaacgggcg aatcggtgac gatgttctac cggcccaaag accgctatct gctgacacag
    19081 cttcgcgaag acattggtgc cgtctacaag gactactggg tcgtgggacc agtccggcgg
    19141 ggctcgctcg cgtggtgcga caagcctccc gccgcgtatg gaaacggctt gatcgaatac
    19201 aagccggacc tccgctcaga ggcgttccgg ctggagaacc cgaagtcgga gggagtagcc
    19261 gtgctcaagg accgtgagaa acccccgctt gcggcagcgg cgccgaatcg tcccgcgtcg
    19321 ctggtggtcg aggtgaacag cccgtttgtc atcgtcgggc tacagaacga cctcacgaac
    19381 ttcgaggacg acagcgatgc tgccgtcgtg agcggactct tctggcgtgc cgacagttcg
    19441 gatgaaaaca ggattctggt cagtacggat gccggccgca cctggaagga agtgtggaag
    19501 aacctttggc tcggtgcggt gccctttcag gtcgacctga gccggcacgt cgtcgggaag
    19561 tatgcctacc aggtgaagtt cgactggatc gaccgaaagg gcagcggcca ggtgggcctg
    19621 gaagaccttc agttcagaac ctgggttgaa ctctcaccga tggcgttacc tcggctcgtt
    19681 gcgggacgca acacgttccg cgtttcgacc agaccccgca gcacgtttta caacgagagc
    19741 ttctggcaga aaggcgagca actccccggc cagcacctcg agaacctcga tacgttcgac
    19801 cagtttccct acctgcgccc acgggacccg caacgcccag gcgtcctcac attcgatctc
    19861 ggtccgaagg gttccgtgga cgaggcgcgc atcagcatcc gtgcacgcgc ggtcggcggc
    19921 aaaccctccg gaacatctgt cacgctggag ctttccgagg acgatggccg gacatggcgc
    19981 ctgctcgacc gattcaccgg ccacaccgag cacgacatga atcacatgtg gttcaaccac
    20041 gtgatcgaag gaaagacgct cgacggcgaa cactgccggc tgcgcctgtc ggtctccgga
    20101 ggcggcttcg agaaggtgat cgccaacagc gccgtacgct cggagccgcg cgtccccacc
    20161 gcgctccgcg tgacccacgt ctggcgcgaa ggtgacgcgc aacggacgtc cgagcacctc
    20221 gttcgccccg gttcaggcag cgacacctat gaggtcgacg ctgccaaagg ccttgtgaac
    20281 gaagccctga ggatcgaagg ggtggcccgg tagcgggggt gccgtaacac ctcgcctcac
    20341 gctttcagcg tggtttctaa tccgcggata acggattcga gatactcgcg cgcgcggcgc
    20401 accagcgcat ccgttccctc ggggtctttc ggccgttcga cgagcggctc gaaaatgttc
    20461 tcgcagatca gcgggatcgt cagccctgtc cgcaagaccg gcatcagcag tttgtaatgg
    20521 tcgatttcgc cgaaacccgg accgcagtcc tggtcttgcg gcgtgttgcg atggtcctta
    20581 atcgcaaatg accggatgtc ccgccagcag gtctggatgt cggggatcgg atcgttgttt
    20641 tcgtagtcga gaacgtttcc ggcgtcgtag cacaacttga cgccgtcgtc ggcgacgtcc
    20701 gcgatgatct tcgcgcactg ctgtcccgtc gccgtgttcc cgccgtgctg tttgatcgtg
    20761 agggtgacgc cgttttgccg tgcgatcggc ccgagcagtt tgagattctt gatccagacg
    20821 tcgtacgcct cggcgcttgt gtgcccgaac gtgatgatga atggaatgcc ggccgcggct
    20881 gcctgctcga tccggcgagt gtgcaccttg accgactcgg ggtcgttgac gtaaatcgcc
    20941 gaaaacatca tcaccggctc gagtccgagg tcgcggcagc gcttggcaag ctcccgggcc
    21001 gcgggcggag gcgcgtccgg cgccatcacc ggcacacgct tcgccgatga ctcgttatgc
    21061 gtggtacccc atccaacgta gcggcagccg gcagcggcga ttcccttcag cgcccgctcc
    21121 agcgggaagg gtgcatacgg aagggtcatg cagccgatct ggaacttcgt cggcgcggac
    21181 tgcgccgcgg cagtggagac gccgaggccc gcccggccaa gcaacgcagc gccggcgctg
    21241 agaatctggc gacgtgtcat ggctatttca gctccttgat tgcgacgttc cggtaatcca
    21301 ccggccagcc ttccgactga aggcagatcg tgccttcttc cggctcggcg tcagaccctt
    21361 cgttgaccag ctttccgttg aggacgacct tgatgcgccc ttcctgctgt gtaatctcgt
    21421 agcgatccca cgagccgaac ggcggacggt ccttcacgat cggaccccgt ttagcgcccg
    21481 tcaccttgcc acctcgtacg ccgaagaggc tgcccgcctc gccgaagtga ccctggacct
    21541 cctgggacat gggccaaccc ttttcgatgg cgccggcgtt gatgaagaag cccgcgttcg
    21601 gccattcctg cggtgcaccg gtccagcctt cggtctggaa cctccactcc gctcgtaaca
    21661 cgaaattccg gtatacctgc ttgctgcgga ggaagccgtt gggcttgccg gtgcagcgaa
    21721 tcattccgtt ctccacacgc caggtttccg gcggggtgcc ttcaacaatc cagaactcgg
    21781 tgatgtcttt cttcggcatc agcgatacga agccgccggt ctgggcctgc gccaaggacg
    21841 ccgccgcaag caatacccaa acaaaagctc gcatgttcga ggtctcctta tggtttcaat
    21901 gtagtgtatt ctctatgccg atggacaaca agaccgcaag acgccacttc ctgctcgctc
    21961 cggcggctgc cgtcacccac ccgctgtggg cgcagaacaa gccgctggtg atcgacgcac
    22021 actgccatgc cggcaaggga acggccatgt ccgccccctg gaccacgcgc gccgatgtcg
    22081 aggtcacact tcgccatatg gaggaggcag gcatcgaccg gacggtcatt ttcccaatca
    22141 acaataccga gttcgagaag ccgaaccagg aggtcgccga gatttgcggg cgctatccga
    22201 agaagttcat cggtttcgcc aaacacgacc cgaagaccga atcgggacgc atcgccaaga
    22261 tgctgcgcta tgaggtcgaa aagcttgggc tgaaaggtct gaaactacac gagctgccga
    22321 cgcgcgaagt gcttgacacg gttgccgaac tcggcatccc gattctgtat cacccctcgc
    22381 gcgtatcgag ctttcacatg ctcgcgccgg agtatccgca gattcctttt atcatggcgc
    22441 acctcggcag ctttgcctcg cagaactggc cggaacacat cgaggcgatc gaagtggcca
    22501 agcggtatcc gaacgtctac ctcgacacgt cgtcggtggt gttcttcaag ttcctggaga
    22561 tggcggtgaa agaggccggt ccggacaagc tgatcttcgg caccgacggc cccgagctcg
    22621 actcgcgcgt cgagctgtac aaggtcaagt tgctcaagct atcgccttcg gaggaagcga
    22681 aagtcaccgg cggcaacatc gtcaaactgc tgccaaaagg aacagtttag ggtgcttcgc
    22741 actcgacttg ggtgacggcc ggttccgggt agtagagata cagcttcgac ttgcgctcgg
    22801 cttggccgat gtaaatcgtg tcgtcggcac cgatcgccat cgagtccacc acgtacgcct
    22861 gccaagtgta gaacggacgc cggttgacat cgatcatccc aagcatctcg tacgcgccgg
    22921 tggaggggtc gtacgaaaac aaacgggcca tctcatcgtc gtcgccaccg gctccgtaca
    22981 gcttgccgtt tcgcgcaaaa acaagtccgc ggatgcggta ctggttgagc ggtttgccga
    23041 ggttctcaat agccgacgtc ttcggatcga gccggaacag gtagccgtcg gaagtgccgc
    23101 cgtaaagcat tccgcaggcg tccttcgccc acgcatcgac cccgttgtac ggctcgcgcc
    23161 cgcggaccgt cgacgctgtg atgccgagct tctcgagccc tgacgccggg tgaaagcgat
    23221 agatcgcgcc gttttcgccg gagctgaaaa cccggccggc atcatccagc accatggccc
    23281 ggccgatgtt cttgttcgtt tcgaacttct caccggggat tttccgctcc gcgaccttgc
    23341 cgtgaaccgt aaacttcccc gagacgatgt cgaagctgaa gaactgcccg ttgggccaag
    23401 tgagcccgta gatggtgtcg aacatgcggt tgatcgccag cgaatagatc ccctcgcccg
    23461 gcgcgggggc gccaaggtcg gtgacctcgc aggcgacgtc cacgcggttc ggcttgtgtt
    23521 cgtcgttctt cgtcacgtag cggtacaggt ggccgtcttt gtcgccggag ccgatgtata
    23581 cgtcgcccga cttcgacaca acaatcgaac ggtgaacggt ggtaaccccg ttcagatagc
    23641 aaagaggctg cacatagccg tgaaccggaa acatgacgaa caggtgactg tgcttcccgc
    23701 tggtggcgcc atatatcgct ccgttcggtg cgaccgtaag cgagcggacc gccgattcct
    23761 ccgacggaat tacgtcgacc ggaggatagc cgagttccct cagatcgatg cggacctggc
    23821 tgacgatcga gtagtcgtgt tcgagcgttc gctgcgcgaa ggtggcgtga gaaagcagta
    23881 gaactgcgag taacagaaac agagttttct tcgacatgtg ttcacctcgg ccggtaaatg
    23941 atcaacgcca ggcggtagta tgcttctccg atcttgccgg ccgcctccac cggcttgccc
    24001 gcctgtggcc ggacctcgat cgcaccgacc atgtagatcg tgccgtcggg tccggtgtcg
    24061 gccgcgttcg tgcccagcac ccggctcccg tcttcgagca gcatctcgcc gaggtcttcg
    24121 gtcttgcccg tgttcaggtc gtatgtgatc aagtgggcgg cggcaaggcc cgcgctgcca
    24181 ccgtagtcga actcgcggcc agcggcgcca tagtagagct tccggtcccg gccgatcgtc
    24241 agcgttagcg tggcgtatgg gacttctctc ttatcagcca tttccggaat cgccagttgc
    24301 cccaggcggc ggatctcgcc gtccgtgcca gcgcgtgggt tgaaggagaa cagaatcgtc
    24361 gcgctctcat ccacgccgta gaactgctgc gtcttggcgt cccaaaccat cgtgcgaaac
    24421 gctgtctcgg acttgttgta atcgcgtccg agcgagatgc ctttcggccg cgtcggaacc
    24481 ttcacggcaa gctcgcggat ggagtcggcc ttggggtcgt acttgaagat tcccccatcg
    24541 ccgaaactgc cgtagacgta gccttcgtca tcgatcgcga gagtcctgca gatcgattcc
    24601 cagttgttga cgcggccctt gtccaccgct ttctgtttcg tcacgtcgta gtagacgaaa
    24661 tggccgcgcg gatgcgtcat tccatagatg cgattgaagc gtggatcgta atttccggtg
    24721 ttcacgcctt cgttcggcac gccaaggccg aagtcatcga cacgcccggt cttcgggtcg
    24781 aacgccatcc agtgggcgcc cgggtaacct tccttggtgg cgaaccggga atagttccac
    24841 caccagccag cgtgagtgcc gaaatagatc cggccgtcct tgccttcgcc aaacttggag
    24901 tggatctttg actgcggccc gcgcttcagt tgagactcgc cggtgagttc ggtgaggttg
    24961 ccgacatcga caacgcggtc cgccttggag tcgtaataca caaggtggcc gtcgccgccg
    25021 tgataagcga gaccggcgta gacctttccg tcgctggcgg caagaagcgc cgcccaattg
    25081 ccgtccatct ggtgcaatcc gggaaacaca cgatggtcta cccgaagcga cgcggcgaat
    25141 agaccagcca ccgagacgca gaatacacaa agaagcctaa ttcgatatct ccaaaaccgc
    25201 cacattgtaa acctccaggg aaggcagcgc aaagcggacg cggccctgct cggccgtgaa
    25261 cgttacgttg gacgcgccgt cgcggtccgg tgaaagcaag tgcacggcct tcactttctt
    25321 cccgcccggc gccgccaccg ttacgctcgc gccacccgcg gggtagccga gtttgtaatt
    25381 cagccagtgc agaattcgct cgccgccatc ggtcttgtcc gtcagttcca taactgtcgt
    25441 aaggggagct ccctcgaatt ccaccgacaa cggccctccc gccacgtatc ggatcgccga
    25501 agcgatctgc cccgcattct tcggcagcat ccagtagcta ttgccgaagc cggctgcgcc
    25561 tgtcgaagcc atgtcctgaa tgggacgcgc cggcgtcacg gctggcagat acgcgaacct
    25621 tccctgcccg aagggtccac gcccttcggc ggggagcgca cgcccaggcg acaccttgat
    25681 cttcaggacg tcggctaggc cgtagtcgcc ccgcacgcgc cgccagtcgt tgtacagcga
    25741 cgtgtggccg gtcgcaaaca ggcccccacc gcgccggacg tagtcgcgga tttgctccac
    25801 cgcacggtcc gatagcgcct cctggcttgc cagaatcagc gccctgtact tcgacagatc
    25861 acccaggtcc tgatcgaaca cgtaggtgaa cggaatcttg tgctggatca gcacctgctc
    25921 gaccagcgta gtttccaggt gcggagcgag gctgttcgtc gccatcgacg ggaacgaccg
    25981 caacaccgcc acgtccgcca ctacgctcgt cgaagcgaaa tgccggttat tgtcgcgata
    26041 gaagcgtatg tacttgcgcg cgtcgtcggg cagatcgtaa gcgttcagcg gcccaccgat
    26101 gtcgccgagg gagtttcggt tgaacgccat cgactctgcc atcaggaggc ggtaggagcg
    26161 cgtggcgggg ttggcgcgct gcgggcccgt gtagctgaac agcatctgat cgaggctccg
    26221 cgccagcttc atcgaacgga tcttcgacac cagaatgcca ctctcgtcga cctgtgcctc
    26281 attcggctcc tcgctccaga ataccgagcc gtgccgcacc agtctggcgt gatccacgcc
    26341 gttcaaaaac gcccggttgc tcccatagat gccgtgcggg ttcagctcca tcacgacact
    26401 cgggtttaga ctccgtgcgt agtccgaaat cttgccgtag tactcggcga aatcctggca
    26461 gcggaaatcg acccactcct gaattaacgg atcgccgatc ggagagatcg ccgccggccg
    26521 agcgatgccg tgccacgtcg gaacggttac ggcgcggatg tcggagaacc cgaagcggtc
    26581 cttcaactgt gcgggtgtgt acttcttcgc cagaaactcg cggaagcgcc ggttgacctc
    26641 cggtgagttg cccgtccacg gcggagcgat aagcgcgtgg ttgtccatgt ggatgaggtc
    26701 cgtcttgacc tcctcgatgg ctatgcggat caccttcttc atgtgctcga cgtagcccga
    26761 atggttgaag tcgggccggt aacggtcggt ctgctccccg taacgcacgg gatcgccgtg
    26821 ctcgtcatag cggatccact gcttcgcttc cggcacatcg cgcagcagcg tctccgcgaa
    26881 gatcgtgccg ccgatgtacg tgccgagctt cagcccgtgc ttgcggcaca gcactccgag
    26941 ttgcttggcg acctcaatgt cttcgcggtc cgagtcgagt cctgtcttgt agaagtgcgt
    27001 gaggatcatg ttcacgccca tctctttgag cttgatgacg gtctcttcgg tatgctcgcg
    27061 ccggtactgc tccgcgacgt ctaccggcag ttcgggccag tgacgcaacc ggaacacgag
    27121 cggctcccag ttgccgacga atacgactcc ctggttccac cacgccggtt cctcgaagtc
    27181 cttcggcacg ggcgtggccc aggtggcatt gccaatctgc gcctccgcgg tggcgcacgg
    27241 gatcagcagg aagatgaaaa cggccagacg tttcatgcga acctcccgcg atctacggca
    27301 ttttcaggcc gaaggagccg tccgtgattc ggaagcgccc ctcggcgcct gcgttcttgt
    27361 atgcggcggc agtccactcg tacgtccggt gagccgacgc catcgggacc gggttcttca
    27421 tcggcccggt cggcgaaatc agcacgaggt tgcggccggc catcgcggcg gcgacgtgcg
    27481 gcagatcgaa atggagcagg acgtcgcgga tgaagacgtt ggcgctgtgt gtgtaccggt
    27541 cgacgcgcgc gaggctgcga taggaatcga gtccgccctc cgcgagaacg cttgtgatgc
    27601 gcgtgtcgag cgcggcggcg tacaacgccc acagtgcgcc catccctttc ccgacaacac
    27661 gtagcccgcc cggggctacg tcttgccggc tcagcgcgta gtccacgctt cgcatcacgt
    27721 cccgcaccct catgccgaac agcgactcgt ccatgtacca tgccatgtac gacatcgcgg
    27781 tctcggcgtc gaacagatga gcgaacggac cgtaccggtc gcccggagga tgcgggggct
    27841 ccgtctcgcc gatgcctcgg acgtccacgg caacgactag atggcccgcg cgagtgagtt
    27901 cctcgagcag gccgaactct gccccgtccg tttccttgcc ttcctcgtgc aagtagagcg
    27961 tcgccgggag acgaccgttc ttggcattgg gaacgaacac ccacgtagga atgtagatgc
    28021 ccggctcgga gaggaactgg agtttctcga tgcggtaccc gttgcgcggt gtggttgtga
    28081 tgttgcgcac gcccagcgga tggtccggct tgtcgcaatg gatcagcccc cggatctcct
    28141 tcgccatctc ggtccgaaac gcagcggcgc cggcggcatt ctcgggtagc atccgatttg
    28201 gcggcagctc cgcctgcctg cgcagcatca gcgagaagac cgtctgcccc tgttgagagt
    28261 accggacaga gccgttcggg gtgcagtaca gcgtctccgg cgcctctgct ttgaaccgtg
    28321 gctcgacctc cggaccgcgc ttgccgagga accagcggca gaaccagtcc gttgtggcga
    28381 tgcggagctt cggcgtccag gagtgtgggt cggcggcttc ctcggtgccg aacttctcgg
    28441 ccacgtcgaa cagttcgtac cgttgccgga tgcggtccat ggcgcggttg aatcctggag
    28501 agaagtgctc gatggtcacc agcagcggcc gcggcgccac ggcaacaagc tggtcgtgcg
    28561 cgtcaactcc gtagatcgcc gcgggaaaaa cgttctgctc gacgtcggag ggacccaggc
    28621 ggctgccggg agagtacccc gacggccagc ggtttgtcgc cccgccctca ttcaccacca
    28681 cgcatttcac gcgttcgtcg atcgcactga tgaacttcgt cagcgtgccg cctccggagt
    28741 ggcccgcgca gccgatcttc cccttgtcca cttccggacg cgtcagcagg tagtcgacgg
    28801 cgcacatgcc gtcccaaacg cggtaatgcg tcaggtcctc tcccatcagg agctgcacct
    28861 gccccggcat ggaatgctcg tatgtggagg cggaggcgac ctccgtcgcg ccggtctgcg
    28921 gattccagta ctgccgccgc tcgccctggc ccaccggatc gaatgcaagg acgaccaggc
    28981 cgcccttcac catgttcatg tatgcgaact ggtactcagg ctccatacgc gcccaagggt
    29041 agtgcccgca gggagagatg attccgggga acggccccct gccggtcgtc ggcacgtaga
    29101 cattcccggt tacccagaag tctggacggc tctggaacat cacgttctcg acccggtaac
    29161 cgtcgcgctc atgaacggcg accacaaccg ggttgagcgg cgcgcgtttt ggaaacccgt
    29221 gcatcatctc gcgaagcttc tgccggacaa aactgttgcg ggcctcgagt tgcgccggcg
    29281 ttttgatcgc ttcccgctcc cgatcccact tcgcagcgag ggcgttcagc ctgttgctca
    29341 agtgcgccag cagcatgtcc ggcatctcgt gggcgtaaga ttcctctcgc gcgccgtgca
    29401 acgcggccgg cgagaaccac ggaaaagtag cggcgacgcc ggcctgtttc aggaatccgc
    29461 gtcggctatt cgtgctcatg aatggttact ccaaaaatcg ggaattatcg agagcatgat
    29521 atcctcaaat tccgctggcc cgcaaaaacc gatgaaaacc cgtctaatcc tgtcactggt
    29581 gcttgccgct tggatctcga gtggccagaa cctttccgat aaactccgcg ccctccgccc
    29641 gtcggacgtg agcgccggcg aggctgcggt tctggacaac ctcgagcggc gcgcgcgcga
    29701 atcgctcgcg gccatcccga gggcacggac ccgtgagcaa gccgacgcgg cgcgcgccgg
    29761 actacgccag aagctcgaat ggtcgcttgg tttccgccgg cttccgtggc ccccgaaact
    29821 cgcggcccgt accgtgggtg tcgtgcggcg cgatggctat cggatcgaga agatcattta
    29881 cgagacgctg cccggagtcc gcgtacccgc gcacatgtac atcccggaca acctaaaggc
    29941 gcctgcgccg gccattcttt tctataacgg ccactggtgg gccgacagca aaacccggac
    30001 ggactttcag gcattccaca tcaacatggc gcggctgggt ttcgtcgttt tgaccttcga
    30061 cgccttcggc cagggggagc gcggcatctc gcgccgcgat caccgaagga ccgaggccct
    30121 gctcgccgga gtctcccagc agggcttcgc cgtgtacgaa acgcagtgcg cgctcgaata
    30181 cctgctatcc cgcaacgaag tagatccgaa acgcatcggc atgtcgggcg cttccggggg
    30241 aggctacaac acgtggatga cggcggcgct cgacgagcga attgcggtgg cggttcctgc
    30301 cgtcggtacc agcgaattct atgagcagat ccacgtttgc cggcctctcg actggtatca
    30361 cgcgagcgag cactgccact tcgtgcccgg cctcctccgt tacgcaaaca accaggaact
    30421 gatcgcgatg atcgcgccgc ggccgttgat gatcgtggcc gcgcacaagg accaaagttt
    30481 ccctgtcgag ggcgtgcgtg aggtctaccg ttatgggcag gagctttacg ggtcctacgg
    30541 cgttccgggg aagatcggct acagcgaaga cgccaccact agccacggtt atcagcagaa
    30601 gaagcgagag gcggcctacg gctggttcct gcggtggttg atgaacaagg gcgacggcag
    30661 tccgttcgcc gagccgccta ccgagaccgc tccatgggac tcggaagagc tgcgctgttt
    30721 cccggtgggg aagaatgacc ccgcgggccc cggctttgtc gaggccgcga agcgcctggt
    30781 gcgcggattg ccccctgcat acagcgccgg cgccgttcag gaggcgctgg gtccgcttcc
    30841 gccgcttcca acgcagcagc cgaggattga gaaatcgccg ttacagcggc tcaaaatccc
    30901 ctcggaaacg ggcatcgtgg tccctgcatt tctgctgcgt ccggagggcc acgagcgcgg
    30961 cgtgctggtc gctgcgcacg acggcggcaa ggaggaactc gccggcgacc cgctcgtcca
    31021 ggccgcgctt gccggtggtt gggccgtttg cggcgccgac ccgcggggca tcggcgagct
    31081 tgccatttcg aagggcggct gggcggctgc cgtgagtctg ctcctcggag agaacttcgt
    31141 ctggcgccag gcgttcgatc tggccaacgt tctcgcctac ctgaacgcct cgcaggactt
    31201 cgccggaaag cccgccggcc tgtatggcag gggccacggc gcggcgctct cggtgacgct
    31261 cgcgctcgcg cagccgcagg gttcgcgcgt gaaatggtac gcgctacgcg acggattcac
    31321 cagcttccgc cagtttatcg agcgtccggc ctcgctccgg gagtcgtaca agctcatcag
    31381 cgacgagaag aagcgcgacg agacgtacga ccgcgagatc ccctatcagg atttcgcctt
    31441 cgacgtgctg cggcacttcg acctgcccca ggtgctcgcc gcggcgaaag caaagggtct
    31501 cgtggtgaac ccgatcgacg gagactggaa ccggatgcca attgaggagg cgaagaagct
    31561 gttgccgcgc ggcgtccgca cggtctcctc ggagaccccc gacgaagaga tccgccggtt
    31621 cctcagcggc ttgtaatcgc gattcctgag agatgttctt gggagccact gcactgagta
    31681 tctgaggccg tggcgcaggc acgtggtgca ggtcatcgtt ttttgtgacc tgcaatctgt
    31741 agcccaagcc ctagctgtca ttggcctgcc atctgccaac tgaagaagat ggtctactat
    31801 cgccgccggc ttccccactg gatccccgct cactccaccc tctttgtcac ttggcgcctc
    31861 ggtggatcct cgcctcccat ccaaccagaa tccttcaccg ctcagaatac cggtcgcatt
    31921 ccattcgccc agcaggatga atggctggcc cgcacgtcct caggtccctt ttggctccgc
    31981 gatccacgtg tagccggcat gctggaggag acacttcact acggggaagc agtccggaga
    32041 atttatgttc tccacgcttg ggtcatcatg ccgaaccatg tacacgtaat tctggagcct
    32101 ggtctggaac tcccgcgggt tatgcattgg ctgaagggac gaactggccg cagggccaac
    32161 cggatactcg ggcgaacggg actcccgttc tggcaggacg aatcgtacga ccattggata
    32221 cgtagcattg ccgaactcag ggacttagtt gcatacgtgg agcaaaaccc tgttcaagcc
    32281 gggctggttg gaggtaaaga acagtggccc tggtctagcg ccgcgacgcc aggcggacga
    32341 cagaatgcga tcgtccgccc cacctgagct gcaaccaccg caggacgccc caggcgcggt
    32401 catccgtcgg ccattccacg accgctttca ccgttccgca cgcataaccc gagccgcgat
    32461 gtcgccccag aactccggcg gcttcatctc caggccgtag ccgaagtgcc ggagatggcg
    32521 gacattcgcc ttgtcgcggc ggaccaccgc tccgctcgat gtatcccacc actcgatctc
    32581 gtagtcgccg tcggccagtc ccacaacgtt gacgctggcg tcggcctgct tcgcgccctt
    32641 cacggctttc ccgtcgatca cgcggaacgc cagcggatcg tgaatccaca caagcgcgcg
    32701 gtccgcggcc atgagcccgt acacattgag ttggactggg aggcttggac gcgacaggcg
    32761 agccggctcc catttacgcg ccgcgaagtc ctcgccgccg acaaacttgg ctagcggcgc
    32821 gtagtgccgg tacaggttgt acgtatcgat gtagttgtgc caccaccaat acatcccggt
    32881 cccagcggcg ccgctcacag cagtcgccca gatgcagtcg tggaagtgct tgccctcggg
    32941 gtcgacgctg accggccctt cgattccttg ttctccgaag aagaacggtt tgccgtagcc
    33001 gcgcatcagg tggtcggtgt cttgaaatgc gtactcggcc gcgtcgtatt cgggtccgaa
    33061 gtaagagtgc ccctgaacga tatcgatttc gggaacggcg aacgtctcac ggtcgcgcca
    33121 gcttgtggtg atgaggtggc gccagggatc gatgctcttc aagtagccgc ccatctcgcg
    33181 gtgccagttc gcacacgatt tcgaatcgaa gttgtccatg ttgtcgactt cgttccacag
    33241 ttcccaggcg gccaggttgg tggtccagcc ccaccgcgcg acgctgtagc gcagccggcg
    33301 ccggacgagc tcgcgcgcgg ccgcattcgt caggtagtcc agcggtttga cgcacggccc
    33361 gccgttggct gcgttgtacg gattgcgggg ccagcggtgt ttcatgctct gctcccacca
    33421 gacgaccatc tcgaaagtga gctgccagcg aatgttgagc cgttcgcaaa cccccgccac
    33481 atggtcgaga cgccacgcag attcgagatc gtagcgcccg aggccggtcg cctgggtctc
    33541 cagcggaaag ccggcgaagc tgccgtctcc ggtcccggcg acaatcttcg gatcctgcgg
    33601 cacgtagtac tcctgccaga gccgcacgta gtttccgccg gcggcggcaa gcttttcaag
    33661 caaccggtcg aagtagtacg tgccctcctt cttttcgtac atgcagaggt tctgcccaat
    33721 cgggaagaac acgcggccgg aggcatcctc gagatactgc cggttgcgtg agctgacccg
    33781 aatgaccccg ggccgcggtc ccgcgcttac ggcaaacgtc acgtcccggc tgcggctggt
    33841 gccacttggg tccttaagtt cgagcacagc gcggtggact ccggtcgcgc cactcgagaa
    33901 acgtacccgc catccggccc tgccttccgg cgtaagggct tcgacgcggt ccgcaccggt
    33961 ggcattcgga ttggcgatgc tccggcgata cccctgaaac cagaagccgg gcacgcgcac
    34021 ctgctggcct gagggcagcg tgacaattgc gtcgagcgtg acgaggttcg ggtcgaacgg
    34081 attccccggc gcccgatccg gcgtcagtgc gatctcgacc atctcgtgct gccgcacctc
    34141 cgacgtcagc accgccggct cgggaagctg agcagcagcg gcgaagagag gtaagaggca
    34201 gaccaccgcc ggggcactaa cgtgtttcac cgagaagttc tccataggaa gccatcagcg
    34261 gctcggcaga ggcgcgccgc cggactttca gattctgcgt cgcggcggtc cggtacgccg
    34321 cctccacgcg ggaggagggc atcgggtttc ggagtgggga gaccgtgttc accagccaga
    34381 cgggtcgcgg tgcaactgcg gcggccaggt ccggaaggtc gtactggtcg agcacgcccg
    34441 ggatcagcgc ctcaaacacg ttccggtgga tcggagccgc tgccaccgcg tggtacgaca
    34501 cgagattgcc ttccaggatc agagcatcga ttcgcgcgtc gagagctgcc gcatgaagca
    34561 gagtcgtccc gatcaggccc ttcgcaaagc cgatcgcccg gccgtcatac aagaggttgc
    34621 gttcccgcag gacgtcgagc ccccgcagaa tttcgtcgat cctgatcccg agaacaggct
    34681 tcccgaccat gagcgccagc cagacactgc cgtcagcgag atacccaggc cccgaatcct
    34741 cgtcgtggac cgcagtctcg ccaataccgg aagggtccac cgccagcacc gtgaacccca
    34801 tccgcgccag ttctgcaaga tccggctgca atgcggaggc acgcgacttc cccgcctgat
    34861 ccacatacaa caccgcgcgc ttccggtcgc gatcttgagc cggcatgcag aggagcgcgg
    34921 gtacgatccg gccgacatcg gccgcgtacg ccactttctc gaacctgaag ccgtcctgat
    34981 ccacggtgcc gaagcttcgg atttcgagcg gagcgccgga gcgctgatag cggctgagac
    35041 tccggatctt ctccgccacg tccttcaccg gccgcgaggg ctgaatcttc tcgagccgcc
    35101 tcacgttcag cgtgctggcg gtctcgcctc cgagcgaatc ggcgacctgg ccagttcttg
    35161 tgcagtacag gtcctcctcg gcttccagct taaatgacgg ctctgaggcg ggtgcgggtg
    35221 ggtcgccttt gagccaccgc ttcatccagg cgtagatcgc ctctcgcgtg tcccgattca
    35281 tgccgtgccc gccggggccg atgtacctcg cgatcttctc cccggctccg aggagcgcgt
    35341 agattcgctt gagttcctca tacgtccgcg tgccgccgtc gagcgggaag aagtcctcgg
    35401 tggtgttgca gatgagatac ggcttcggcg ccagcgcaat cgcgaaatcg ccgtggttca
    35461 acccgagccg caactggtct ggaaactgct gctctgcgtc ctgcggtcca gtgccctcga
    35521 gttgctcctc ccacgcgctg atataacacg cgggagccgc cgcctgaacg cgatcgtcca
    35581 gcgcggcgat gtaagccgtc agcgttccac ccccggagca gcccgagacc ccgatccgtt
    35641 tcgggtctac ctcaggcagc gactgaagca gatcgatgcc gcgcatacca tcccaaatgc
    35701 ggtaccgcgc cacgctctcc ccggccagca ggctctggcg gcccaccatg ctgtgctccg
    35761 ccgtaggacc cccgactttg gagttctcga tatcgccgtc ccagaagatt cgccgctctc
    35821 cctggccgat gggatcatag gtcagcacaa cgaaaccata cttggcgagg ccgatcgaaa
    35881 ggctctggta gatccctccg gccttcgcgt tcatgctgtg tccgagcggc tggatcaccg
    35941 cgggataggg cggctggcct gactgcggaa tatagaggct cgcggtgaca taaaagccgg
    36001 gcaggctctc gtagatcacc ttctcaacgc gatagtcgcc gcggtcgagc cggccgatcc
    36061 gcttcaaatt cagtggcgtc cgctcggcgg gtaacccgcc aatgagcttc agaagcgtcg
    36121 agcgaacctt cgcgcgtcgc tgctccaact gctcaggggt ctttatgttt tcaatctccg
    36181 cccggcgctg ggcgagttgc tggtcggcca gcccgtgcag gtaggttgtc aggctctggg
    36241 gccttgcggc cacgggatcc agcacgcgga aatcctgcgc cccagcaagc gtgcaggcaa
    36301 gacagaacaa ggttgccgtt ttcatggtcg tcgaatggtg attgtacgtc ttcctttgcc
    36361 aggatcgcgg aaggagagga caccttgtcc gttcacccgg ttgaaggcca gtctcttgcc
    36421 gttcaccgcc acttccaccg gaatgcccgg cggcattccc gtcaccacgt gacgtgctgg
    36481 tccgtccgcg gcgacttcat aggtcaccgg caaggtcgct ggcccgccgg cggcatcgcc
    36541 ggacagcagc acaacccggt tttccgggcg cgacaggaat tgaacgccgg tgagcccgcc
    36601 cgaaagcgcc ttgacggagc ggacttccac gggagagccg gttgaaccgc cgaccatctg
    36661 gatcgcatga aggaacaggt catcttgcga cggacgctcc ggcgccacct cgatccgcca
    36721 gttccccgac tcccgggcgt ctccggcgac tgccggccgc gaggtcgggt agttcacgcc
    36781 gaggaaagag ttgaagtatt cgtagccagg gccgccgatc accgacacca cgcgctgctc
    36841 gggcagaagc gtgtgtacaa aaagggagcc gtcgtacgta atcggccttc ctccaagatt
    36901 cggctgaccc tggcggcgaa ccgcggttag ccggccgccc ttgaactctg tgacgcccgt
    36961 ttcgggtgcg gctccatcca cattggggcg ttcctggaag tgcagcagcc agcgcttgcg
    37021 aaagtctccg ttggcggcgg tgacgcggtc gagaaccacc aacaggtcct cggacggtag
    37081 ataaacaaac tcgcggccgt agcgggtcac tttcggcccg tatgccggcg caagatcgac
    37141 ccggacgtag ccgtagccgt cacccgcgtc gaacgccgtc acgttggccg tatcgaggcc
    37201 ttctttttcg cggtgggcga cgtaggcagg ccagtcatcg tggtgctgct tgccgcgaag
    37261 gacattctgg cctccatcgt tcgtgtggcc ctttggcaat tgctggtcgg gatcgtaaac
    37321 caagagacag ttatgggcca gcgtccgaac ggcgtattca ttccagtggc cgccggcttt
    37381 gtacatctgg tcgtacgcac cgccgtccac cgtcaggccg ccgcgcttgt agatcaggaa
    37441 gtgacctttg tcgaagtgct gatggtcggt gaaatggtcg cccgccagaa tcgtgatttg
    37501 cgtgctgtcc tcattccatc cgttgcgaag gacgatctga tcgtaggttc ccactcccat
    37561 acgcatcgcg aggggaaggg tccggaaaga gggtgtctgc ctggcccgcg gatcgtggaa
    37621 aatccgcatg ccccacccga aggccggatg gaccgccttc tcgccgtaag cctcctgtaa
    37681 cgcacggtcg aaggaccgca acctggcggc gtcgggatga tctccgagtc cccaagcgag
    37741 catctcgact gtgaccggca cggtgatgtt ggccatcgct cgggaaggcg agcaatcgcc
    37801 gatgcgctcg aaggtgaagt cgggccgtgt gcaggcgatc aggaaacgtc cagcaagggc
    37861 aagaaaatcg ccgtgatcct tcctcgcggc ggcgaagtag tcccggtcgc ttgccgtccg
    37921 gtacgccgcc aacatcagga cgagattacg aagggtttca tgaaacgtgt aatggcttcc
    37981 ctctgcccac gccccttccc gagcgtgcca cgtgtcgagc accatgcctg gagactccag
    38041 gaacccgtgg ctcagttcca ggtagtcgcg ggccgtactc ccgtacggtt caggctcgcc
    38101 gtggagggcc agaccgcaga tagccacagt ggccagcgcc atgtacgtgt agttgtggtt
    38161 aatgtccggg ccgccgcgct ccagaaaccg ccgggagctt tccgcggtgg tgacaatgtt
    38221 cgccattgct gacttgcggt cttcgccgct gagcccgccg tagacccagt cgaacgcggc
    38281 cgccatctcc gcgcccgcga gaaagcctcc gacgtcatgc ttttcgtagg agaacgtgtt
    38341 tgttttcagg aactcccgga cggtggcgag gtcggcggtg ttgccgtcct caaggtatct
    38401 tgccgcgcgc tccacagcag cgaccgcacc gcgcgcggag atcgttccgc gccatcgcgc
    38461 ataggccggg ttcttcagcc tctctcgcaa ctgcgagacc gtcagccccg ttccgatcac
    38521 agccttatca tgccggacga aaatgcgtgg atggagtggg gagacctccg cggcggcagc
    38581 caccaccagc ccgagcgcga ggaacactga gaagcggagc atagaccagc atcatagcct
    38641 cttgttaccg tctgggtggc tgcggccaag gaccaaccgg cgccgccaag tccgaattga
    38701 gcggagtggg agcggcgtcg cggatgggct gccttaatgc ccatgcgacg accggatacc
    38761 ctctgctata ctgcccgatt cgatgtctga acgcgccgcc gacacagcgt tagagtccct
    38821 gggttacaag ccgaagttca agcgggtcct gtcgttatgg gacctgattc tctacgggct
    38881 ggtgatcctc acgcccaccg cgccgtatcc cgtgtttggg atcgtgcaac aggtttctca
    38941 cggtcacgcc gccctaagct acctcgtggc aatggtggcg atgatgttca cggccgccag
    39001 ttacgggaag atggcgggcg catttcccgc ggccggatcg acgtacacgt acgcccagcg
    39061 ggcactgagc gagcacgtcg gtttcctggc tggctgggca atgattctgg actatttcct
    39121 gattccgctg cttagcgtaa tctacgcgtc gctcacggcg gcgcgactcc tgccgcaggt
    39181 gccttacata gtgtgggcgc tgatgttcac ggcgctgatc acgatgatca acgtccgcgg
    39241 cattcaggtg acggcccagg cgagcaaagt catgatggtg atcatgacgg gctgcgcgat
    39301 cctgttcgtc tggctggcgg cgcgctgggt ggcgatcgaa cacggctttg gcgggctgat
    39361 caaccagatc ggcatgtacc gcccggacac gttcgcgatg ggcccgctga tgctgggggc
    39421 ggggattgcg acgctgtctt acatcggatt cgacgccatc tcaacgctgg cggaggacac
    39481 ggtgaggccg gagcgcgata tcgccatcgc caccgtactg gtttgcatcc tgcagacgtt
    39541 tttttgcttt ctcacggtgt atctggcgac gctcgtgtgg cccgattaca aatcgttccc
    39601 cgaggtggag acggtgattc tggacatcag tcgaacgatc ggcgggccgt ggatgttcgg
    39661 cttcatcacg ttcgtgctgt tggtggccgg gctggcgagc gctctgaccg gccaggcagg
    39721 ggcttcccgt ttgcttttcg ggatggggcg ggacggcgtg ctgccccaac ggattttcgc
    39781 ctacgtcaac ccgaagtatt cgacgccgac ccgcagcatc tacttgatgg ccggcgtatc
    39841 tctcatcggc gcaggtctca tgcgattcca gctcgcggtg gagttgttga atttcggagc
    39901 gtttgtcggc tttattctcg tgaacttgag cgtgatcgcc cactatttca ttcggaagcg
    39961 cgagcgaggc ggcatgcggt ttctgaccaa cctcgtgttc ccggccctcg gagcgctggt
    40021 ttgcagctat gtatggatga acctgacaac gaaggcgaag ctggttggat tcgcgtggat
    40081 gggactcgga gccctttacc tcgccgtgat taccaagggg tttcaggtgg cgccgaagtc
    40141 gctggggagt ctgacggaag gagccggtga tggcgcttag tcccacactc gaagaacgaa
    40201 tctgttccta cgtcgattcg aaccgcggaa ggcttgtggg gatcatccgg gatctggtca
    40261 ggattccatc ggagaacaca ccgcccgtgg gagcggaaag ggcgtgccag gaatacattg
    40321 cggcggtgct gcgaaacaag ggatgggaac cgcttcttta cgagccgacc gaggcgcccg
    40381 ggctgatcga gcatccgctg tactggccgg gacgcgacta caggaaccgg ccgaatctgg
    40441 gcgcgcgacg caagggcagt aacggcggcc gctcgctcgt gctgtccggg cacatcgaca
    40501 cggtcccgcg cgggacgcag aactggacgc gcgatccgtt cagcggcgat gtcgagggaa
    40561 acctgctcta cggccgcggc tcgaacgaca tgaagggcgg tgtggggacg aacctgttcg
    40621 tagtcgaagc gctgggcgag ctcgtgatcc ggctggcagg cgaccttgtc ttcgagacgg
    40681 tcgtggacga agagttcgga ggagtgaacg gcacgctggc gggcaggctg atgggattca
    40741 acgccgatgc ggcgatcatc tccgagccgt cattccttcg gatttgcgcg gcgcagcgcg
    40801 gcggacggac ggcgcacctc acgttccgga ctccgcccaa cgccggagtg ctgacggagg
    40861 gagagtttcc ggccggtgta tcggaccagg tgggctattt cctgcgcgag ctccgcaact
    40921 tcgccgaaca gcggcgcaag gccgcgcgaa agcacgagat gtacgaccat cacactgacc
    40981 cggtgccagt ggcggtcacg aagatctcca ccggaccttg ggggacgaag gagccggtca
    41041 cgattccgga cgagtgcaag gtggaaatgt actggcagtt gatgccgggc gaggtccagg
    41101 aggaggtgga acgggagttc ttcgaatggc tggaccgggt tgtcgcgtcg gcgcccaagc
    41161 tgttcgtgaa ccggccgaag gtcgagttcc ccatccggtg gatgccggga tcgtcgattt
    41221 ccaggagtga gccactcgtt accgaactcg cggcttgtgc tacgcgggcc ctgggtaaag
    41281 agcctccgat cgttgggatc gagggcccgt gcgacatgta cgtgttccat caggcgttcg
    41341 ccacaccggc ggttttgtgg ggcgcccggg gagggaacac gcacaacgcg gacgagtacc
    41401 tggagatcga cacggcggtc gccgccgcca aatcgttgct gctattcgtc tgccgctggt
    41461 gtggagtagc ggggtagggg gctgggcgtt tgcagtcaat ttgacggttg ggtgagcggc
    41521 gtccgagtag gcgcgtttcg cgaccacaag tggtggtacc tattgtggaa gcttcagctc
    41581 gtagacaatg gacttgccaa gtgagtcata gatctcgaag tgcggcttgt tgtcattgtc
    41641 gacgtagagc ttgatggcgt tgcggcccca cttattccta atgaagacac ctgtatcgtc
    41701 ataagattta ctgttcttga tacctcgcct cacaccgaag aacaaacggt tcgagctgat
    41761 cttgcctgcc tgaagggatt tcaactgaag ctcctcccgt tgctccggag ggagcttctg
    41821 gagctcgcca tagatggcgt actcctcctt gatgcttgtc cagtcgggac gagcattgat
    41881 actcagtccg tcttcaatgc ggaggccctg ctggtccttg aactcctcat ggtggaggta
    41941 aacgttctgg tcctgacgga attgatccat tgtgagagtg acatctgaat cctgcccgcc
    42001 gggggtggtt ctgccgtaat agaccaagcc gccggtctcc tcaccttctt cgttgtaaaa
    42061 catcagacca gcgaatctca cgccccccat ctgcgcgcgc tgaccaacat tgaatcccgc
    42121 cgagttcgag agggctgcct tgagcgtccc gctcttctca cgaatgttga tacgctcagc
    42181 gtcgatgacc ctgaatcttt gctggggcaa gagtggatag aagaaattcg cgcagagaaa
    42241 tgcagttgaa agaaacaaca caaggacgca gagttgcagt gcacgaatct gacgcacaag
    42301 cctcgtttcc atcgtaagcc ccctcattca cggacgttga aatcatacct caaggaactc
    42361 gttgtcgcgt tggcgcctaa gctgttcgca agccggccga aggtggagtt tccgattcgc
    42421 tggatgccga gctcgtcgat ctcgaagggg gaaccgctgg tttcggagct tgcggcgtgt
    42481 gccacgccgg tgctggggaa agagcctcca atagtgggga tcaaaggccc gtgctacata
    42541 cgtgttccac taggcgttcg ccacgcccgc ggtgttgtgg ggcgcccgtg gcgggaacac
    42601 gcacaacgcc gacgagtacc tggagatcga cacggcggtc gacgcggcca agccgctgct
    42661 gttgttcgtc tgccgctggt gcggtgggcg gagtgaagga ggcaaaagaa gctcgggact
    42721 gtagcagagc ttgaaacggc tgcgcacggt gctgttgggt tctgaagacg gtattatgtc
    42781 agcgaagcaa tggtgtcaag agcctgaaga agccgtagac aatcagcctc atcccattcc
    42841 ggtacgtcac gtccgtgaag aatccagtgt cggttgagcg gcaaacaggc cggatagctt
    42901 tagaaccacc tttcgcgggt gcgcgagccc agagagactt gtcgtcagcg tgggaaaggc
    42961 gtcgtccgct ttgggcgctt tcacctcgac ggcacgggag acacgggcga agatgagcgt
    43021 caggagcaac ccaacacagg catacagaat caccacggtg cggtatcctt actaatgcct
    43081 ccattcttcc tttaactaca atcggaatca tgccgccgca gagggctccg acggccgttg
    43141 caaacgcgcc cgtaagtgta taccaggaaa atgccgctgt cctgcaacgg ccagcaacga
    43201 catgggcgag cgcggcctgt tcaatcggca ggaacggacc tacctcgttg ccactcggac
    43261 tcacgactcc gataattccg gcgatgacca gcatcaaaaa gttccttgtg gaagcgaaag
    43321 ccagacccgc gccaaccatg agcagggcgc caaccaccag cattcggcgg cggcctattc
    43381 ggtccgcggc ggttgtcagg aacagggaaa tgaccgtgtc tccgaccagc gtgagggtga
    43441 aaagcagccc agtctgcgac tcactgagcc ccagcccagt aaggtagaac gccagaatca
    43501 cggacagcgc gccgtatgcg aagaggcgga taaagcgagt caggaaaagc agccctgcgt
    43561 tgtgagttag gtctcagagt agatttccgt acagggtacg attccccctg cggcgcttca
    43621 ctccacctcg tagattcgaa tctcagcggg acgtgttccg tcccgccgtc ccgccacgaa
    43681 tagccgtcga aggtctgggg agaaccatcc ggtgcgcgct cccttcgcag taccgatctc
    43741 ggcgagttca ttgtagtgat caaccgtcag ttgttggaag acggaaatcg cgccctctcc
    43801 accaatcgca tagatacgct tgcgggccga atcatagaac acgtcgtcgc agtccccgac
    43861 ggcgccaacg gtctccacaa cttctccgtt gagcgtatcc agtacgacca tacgagcggg
    43921 actccggcag actacgaaca tcctgtgatc cgcctcatcg agagccatcg gataattgga
    43981 ctgaggcccg cccgttgtcc aggtggcgat gaccttcctg gtctcccgat ccaccacagc
    44041 gatcttcctt gaccctggaa gatttacgaa aatccgagat ccagttgttt cgagctgaaa
    44101 agactccgga tgcgcgtcga gcgggataac accgatcttg tctccctctt tatccatgac
    44161 accgagcgcg ccgctcccgt agcccacgta aatacgatcc cggcgaggat catagcgaac
    44221 gttatccgcg tcctctcccc acggaatcgt cttcagaagc tggaatgacc ttccgtcaaa
    44281 gatacgcagc gacccatccc cgccgtttgc agcgtacagg cgatttgtgt ccggcacata
    44341 aacgagaccc tgcggctcct ggagaccagg aatgctgtac aaccgtttcc cgttcttcac
    44401 atcgataact tcgatcgtgt tgttcccaag agctgccaca aacaggcgct gccctttgac
    44461 atcgatggac aggtgatcga tccgcccttc aacctgaggc agcggaatag ttccggccag
    44521 gcggagcggc tcgctgtgct gagcctgcgc cagcgcaata acagagaaca ggcacatgag
    44581 aagccacatt ccatcctcct cctagccgtt tagggggctg cttcgagaga gggaaccgca
    44641 tcctgggcgc gcacactgga gacggggtgg gtgtgcggag cgggatgttc cttacccaat
    44701 tggtccagcg ccgctctgaa gctggaggcc agtttatctg ccgggcctcg gccccagtaa
    44761 tgcaagaaga acaccatggg ctgcgtatcc gtcatgtgct gatggatggc caccacatcc
    44821 agcccgtggc tgcgcagggc ttttagcacc ggcgtgatct cgttgtccag cattgccacg
    44881 tcacctgcta cttccgtgtt ggcgtctgtg ccgtagaagg cggcccaggt gttcagcccc
    44941 atccgtgcat tgaccggcgc gcccatgtcc accagcttca agtcgtcccg gccaacggtg
    45001 atcttgtaga ctggtccgac ctcttcaccg cagtgtccaa cgatctgcgc gatcttcgcc
    45061 ttatccagct tgcccgggac ggccggtgga tcactgggtt ggctgtgcgt cggcacatga
    45121 ccgatcagat cgagagccgg cttcactcgc cgggcgagat ccgcggacgc tcccatcccg
    45181 tggacgtgca tgtagtacat gcgcggctcc tcccaaaaga agtggttgtg cagcgccgtt
    45241 acttccaacc cgtgatcgag caacgcggac atgacgggat tcacctcgtc ctgtgtcagg
    45301 accagatctc ccatcatgac gtccgagcca gcccggttct ttgtcattgc gacccagccg
    45361 ccgaaaccga aaggtgtggg cgtggccacg ccgtcgacgg atacctttat gtcatttcgc
    45421 gggatgttca ccttaagcac gttgtctttg tagtcgcccg tcttgccaag gtaattcaac
    45481 acttcttggt agctcggtgg aatctgttgc gcctgcgcgg gcaggcttcc tccggccagc
    45541 caaaccccac acaagacacc cagggcaatt cgtgtccgat gttgcatctt cccctctcca
    45601 gtagccgctg cttttatttt agcgaagctg ggccggcggg cagaagagta cctgccagtc
    45661 cagcggtggc tgcctgaaga agacgcatca gtagctctcc gccgctgcca atcgccacca
    45721 ctgctctgag catcgagaga aggttgagct gtggggcgcc cggggaggga acacgcacaa
    45781 cgcggacgag tacctggaga tcgacacggc ggtcgccgcc gccaaatcgt tgctgctatt
    45841 cgtcagctgc tggtgcgggg tgagacagta acgcgcctcc gacgcgagag gtccgcttcc
    45901 ctggcggact ctagcgctcg acttccagta cccgcagttc ctcgaggagt tccaggatgc
    45961 tcaggacctc gacgcggctg tcgtcggaga gtccggcttt ccgcatgatt tcgtcgattc
    46021 tcagaggctc ggcggtcggg aagggcagat ggtagaggaa cttgccctgc gcggccgtca
    46081 gattgatttt cctctgtttg cgatctctcc cctgaccagt ccctacaagg atactggcta
    46141 actgctgccc gtccctcgac gtagaaaagt gcaccgcagc gttccttcgc tctcgaatcc
    46201 tggcgctcac cttctcgtcc tgtttcccgg ctcgacgcag agaagtcaga tcaagctcca
    46261 gttgaatctt ctcctgctgg agcgagtacc ggtagtaagg ccgtttccct tcgcggactt
    46321 ccgctctggc cacgatgccg aggtcggtca cggtttccag gaaatcctgg gccgtcttaa
    46381 tgtggagatc cgttcgagat gccgcctcgg acgccgagat atcccggtag ttggcgagca
    46441 ggcggaacat ctcctccgcg tggtccttcg cgagacacgc cccgagtttg gctgccttgg
    46501 agaagtccat ggcgaatcaa gagaagacac cgacccgcat cgcatccagc ttgtcgatga
    46561 tgcggccagc cttacctgcg cggtagagtt ccacaaaatc ggtaagaacg cgcgccagcg
    46621 actcacataa gcggtcgaag gccagttcgt cccgataggc ccggtagtcc ttctccgaga
    46681 tgttcaccga gacgccgccg tcgttcgcga atgccaccca ggtatctcgg gagtaatccc
    46741 gccgggctgc aaacaggtgc ctgtattcgg cgcccatcgt taccgggaca aacactaccc
    46801 gttcgagctc cacgtaaggc attgcgatca ggttcctacg cgcaaagccg gcaaaggcca
    46861 aaacgtcgtc acgggagaat tccgtcttcc ccacaggctt gggacgcgga gtaaacccat
    46921 cggaagttgg ttcaaccggc tgtgcggcca gttcaagaat gtcgcgaccg cgccacttgg
    46981 cctgcctggt cagaacaagg aacagcagcc agacgagggc agcaacaata aggagcccga
    47041 gcgccggcaa tctgccgtgg acgactggaa tttgtccggc ccgggagcca aagtaaaccg
    47101 tgaccgcgga caggtagagc gcgctagcga ggtaggtttt gctgcgcgtc ctcgcaaacg
    47161 cgatccacgc cagcgcggaa aacgctacga acaataggaa cgcccacaat atggctgcgc
    47221 ccttgccaaa cagaaacatg acaaccgcca aaagcagcag agtgcagatg gcgggcagta
    47281 gaccggtaat cgaccggtcg gcgccgagct tgctcagttc tttcatttcg ccatacctcc
    47341 ctccggatga cccggggccc caacggccac tttgtgtatt tccagtatag ttcattatta
    47401 gtgaagtata ggaataatga attttcatgt caagcgattt attgggggcc gtgtggggga
    47461 ttcttgcccg ccgagttcct gggacgagtt cagcgtcaag cggagaagcg gcggaaggcg
    47521 gcgcggaagc gcgagatgta cgatcaccac tcggacccgg tgccccagcc gcagctttgg
    47581 ctgatggagg attttctttc tttggtgaag ggcaggaagt aagacggtcg catgagcctt
    47641 tcttcgggtg cggcgtcgcg gtggtcttga cctgttcttg aaccgagctt gacctgaaat
    47701 tgatgctgct caggctgggt catgctaggc tggcggtggt ggctccgttc gagcttctga
    47761 aggtgctctc atgctcgcta gagcccgcca gaatcttgcg gctgaagccc ggcaacggtg
    47821 tcgtagccct gtttctcctg accgttctgc ttccggccgc tttgctggcg ttcctcggtt
    47881 ttcgagcttt ccggcaggaa cggcgactgg ccgatcacca agtccgggac cgtctggaaa
    47941 gagtggccga gtcggccgcc cgaaacctcg aagaggaact gcgtgaatgg cagagcgcga
    48001 ccgaggcgat ccggcccgac cggacacgct cgatcgagga actcccggca cgaatgcgcg
    48061 acgcggtccc cgagccggat tcggcggcgg tggttctgcc cggacagcgc tgctctcgcc
    48121 gattccagaa gcccaaccgc tatacgacct tcttcagagg gttgggccat ggagatcttt
    48181 ctggttggcg atttgccgtg ccgcccgggg ctttgagact cgcagagctg gaaagctccc
    48241 gaaagcgccc gtagtcttca gagatccggc ttcggaagcg aatctttcaa cgttctcaac
    48301 caggtcaatc tcacccgtac cagaaccttg ctcgccacct ccgatgccat cggggacgac
    48361 ctaacacggc gagccagcct tgacagctaa ctgtatactt agcccgtgcc aaacccgagc
    48421 gccagatgct gcgaggttgc gctcggtcgt gaatgggaat cggggtagga gagtcacatg
    48481 tacaacaaga cgctgctcaa cattatgatc tggttgtttt ttcttggcgg ccttttcggg
    48541 ttcgtcacgg gaatgtcgaa gtttttcggc gggggaacac ctgctgagta cggcgtgatg
    48601 gggattggtg gaggcttctg gcttctctgt tcagcgatta cgatccacat tcgtactcgg
    48661 ctacagtaga gccacttgac ggggtagccc cacaactgta aagctggcct gctgccggtg
    48721 gaggacttcc ggtagcggcg aaatacattc ccaaagcgcc cgcttcatcc cgagatccag
    48781 ccttcgggag gcagagccgt cgtcatgagc cgaagattcc ctatctcggg agtgaactga
    48841 ggaatcagcc atgttaccgc cccacgggca gcggaatctg cgcgcagagt gtccgtgcgc
    48901 actaatgcct attgcaggcg cgggggatca gagttagcgt tgcggtcgga aggagggttc
    48961 tcgtggttga aagaacgtcg agaaccgcac gcgaaattcg cgaggaggac gcccccactg
    49021 ctgaagcatc gcttgattcg tggaaagaga tcgccgccta tctgaagcgg gatgtggcca
    49081 cggtgcggcg gtgggagaag cacgaaggcc ttccggtcca ccgccatcac cacctttcgc
    49141 gcagcagcgt ctacgcctac ccgagtgagc ttgacgcttg gcgcgccaat cgaagacccc
    49201 ttgcagaatc aagacattcc acctggtggc ggccggtgcc ggcctttgcg tccgtgatca
    49261 cactggcgct tgctctgatg atggtcggca gcgacgcgca gatgggcggg tcggctcagg
    49321 cggccgagcc gaggttccag cgcattgagg tgcccactgg gctggatagc atctctgcac
    49381 tttcgcccga cggcagcaag ctggccttcg tctccggtgg ctacggcggc gaggtctgag
    49441 tcgtccccgt gcacgggaaa gtctctcccg gcatcgcggg tgagccggaa cagcttaccc
    49501 atggtgcgga ggcgatgcag cttggtttgg catggtcggg caatggccaa tggattgcgt
    49561 tcaaccgcag ctcggcgaag gccgccggcc gtctgagata catctggttc cggcaatggg
    49621 cgggaccgca aagaaggtcg atcttcccga cctgagcgac cgggtgatgg agaacttcct
    49681 gcagcagaca gcggtacagc gacaagatcg ctgagcagag gtgccggttg gacacagtcc
    49741 cgatcgaccc aggtctccta ctgcagtctc tctcgcttat aggtctcctc tagtctgttc
    49801 gggtccgctg ccggaccacg gaggcgacga gtgggctgca accctgcagg cgttccgctt
    49861 gccgctgagt ttctgggctt cactggggaa catgaagctc cctaaagagc ctccgatcgt
    49921 cgggagggcg ggacgctggc agggcgcgcg tcgaaatgaa atactgattt cgatatgcgc
    49981 gcacttttgt tctgcctcct tactgcaact tcctctttct ctcaacaggt caccgatctc
    50041 tggcttagag gctactcggt catccccact cctcaaaccg tcaagctcga atccggcgag
    50101 gtggttcttg gcgcaggctg gacgattgac ccagggaaag tcaatccgca gaatatcgcc
    50161 gtccggacgc ttcggcagga tgcggccgag tttcatgggc tgaacattgg ggtgaagtcc
    50221 ggtgcgggga aggtgatccg tctcgcggtg acgcccggag cggtcgcgac gaaagccgat
    50281 ccccagatcg acaagcaggc gtaccggctg cttgcgggtg acggcgtgat cgagatcacc
    50341 ggcaacggcg aacctgggtt gttctatggc gttcaaactc tgcttcagtt gatgaagcgc
    50401 gacgccgcgg ggcggctggt cataccgaag ggatcgattg aagactggcc agcgatgcaa
    50461 ctccggttcc tccactggga cacgaagcac caccaggacc ggatggagac gctgaagcgt
    50521 tacctcgact gggcagcccg gttcaaagcg aacatgatcg cctttgaatt ggaggacaag
    50581 ttcgagtacc cgtcacaccc ggtgatcggc gcgccgggcg cgttcacgac ggcggaactc
    50641 caggagatcg tgaactacgg gctggaacgg cacatccagg ttgtgccgaa cgtgcaggcg
    50701 ccagcgcacc tggcgtacgt gctgaagcat ccggagttcg ctcatctcaa ggcggacggc
    50761 aacaactacc agtcggacct gtgcgaccca aggacgtacg agctgatctt ctcgatgtac
    50821 gacgacgtca ttaaagcgag caagggcatc gattacttct atgtctcgac ggacgaagtc
    50881 tattacgcgg gcatcaacgg gagatgcacg gcgccgtaca acgacgaaaa ccgtagcctg
    50941 aagtgggtgg agttcgtcaa gcgtgctcac gagcatctca gcaaacaggg gcgccgcatg
    51001 ctgatctgga tggagtaccc gctgttgccc gagcacgtga agatgctgcc ggccgacatc
    51061 atcgacggcg tgatcggaga ggaagagtac ctggagacgg agaacaagat ggggatgcgc
    51121 cagcttggct atgtctccat gcaaggggcc gagtatctgt tcccgaacca tctgaaccgg
    51181 caaagccgag acggggtggc tcgcggacac ctcgacggcg cgttccagtc cctcgcggcg
    51241 ggccgctacc ggcgcggcaa tccgatcggt gtgttcggcg cagcctgggg ggactccggg
    51301 ttgcacaacg agacgttttg gctcggctgg tcggcggtgg cgcagtgggg ctggaacccc
    51361 ggcacggcaa ggccggccca acacgccgcc gagttcatgc gactctacta tggtccgcgg
    51421 gcggagggca tgcttgaggt ctacaggtcc atgcagaacc aggccagctc gtggcaggcg
    51481 acgtgggacc gtgtggtctc ccgcgtgcga gcgccgggtt acggcaattc gtacggcaag
    51541 gggataggca cgacgcgccg cgacgagacg ctgtcgctgc caccgctgcc ccaagcgggc
    51601 gatctgcgaa tcgagccctc gttcgcgaag aagtacgaga agtttctggc cgaggcgcgt
    51661 ggccgaatgc tcgaaaacga acagctacag acggcgatcc aggctgagtt cgggctggcg
    51721 gaccggaacc actacaacct ggaagtcctg ctcgggctga ctcgtttcat cggtcaccac
    51781 tcgcggttgc tcgacggaat ggatcgtgcg gagaaggcga tggagcaggc gcgcgactcc
    51841 catcagaaga aggaatacgc agaggccgtg ggacacctgg tcgaggcctt caacagggtc
    51901 gatggagccc ggcgggaagg gatccggacg ttcggcgaac tgcgcgccgt gttcgagaag
    51961 agccagttcc caaagggccg agcggtaggc ggccgcaagt tcgtccacgt cctggacgac
    52021 accaaggacc attgggcgga ccgaagaccg gacctgagct atatgtcggc gccagaggag
    52081 agcatcggca tggaggagtg gctgaagagt cttcgttcga tcacggaggc gtatgcaaag
    52141 cagcacaatg tgccggtgcg gggcatcgcc gacgcgcgcc tggaggagta aatgcggcgc
    52201 gcgggccttc tgtgcgtgac agcggtattg gcgctcggtc aggagccggg tacgctggcg
    52261 gaacgcggat attcggtgat cccgacgccg cagagggtgc aactggcggg cggcgaggtg
    52321 gaactggact cattctggag cctcgatgca gggaacagcc gcgcgcaggg aatcgcggtt
    52381 cggtgcctgg tcggcgactt tgcggagttc catagggtca gcctgaaacg cggaaccggc
    52441 aatcggcaga tccggctggc aatcgttcca ggtacgatcg tcacatcgct gggagcggaa
    52501 accgaccggc aggcttaccg tctaaagatc gatggcagct cgatcgaggt gaccggtaat
    52561 gacggtcccg ggctcttcta tggggtccaa acactcatcc aacttccgaa gcgggacggc
    52621 gcgaaccggt tgctcattcc aaagggcgta atcgaagact ggccggatca cccgctccgt
    52681 atcctgcact gggacgccca gcagcaccag gaccgcgtcg agacgctgaa gcggtacctc
    52741 gactggacgg cgcgattcaa ggcgaacatg atcgccttcc aactggagga taagttcgag
    52801 ttcccgtcgc acccggtcgt gggcgcgccc ggcgcttaca cctctgccca gttgcaggaa
    52861 ctggtggatt acggtctcga gcggcacatc cagatcgtgc cgctgattca ggcgccggcc
    52921 cacttttcgt gggcgctgaa acaccccgag ttcgccgagt tgcggtcgga tgggaacaac
    52981 tacatggcca acacgtgcga tccgcgtgcg gtgaactttg tcttctcgct gtacgacgac
    53041 gtgatccgcg cgacaaaggg agtcgactac ctgttcgcct cgaccgatga ggtctactac
    53101 gccggcatcg atccacggtg cgggaagccg tacaatcccg agaaccgcag cctgaactgg
    53161 gtggacttcg ttcagaaggc gagggaccac ctggcgaagc ggggccgcac gatgctgatc
    53221 tgggccgagt atccgcttct gccggagcat gtaaagctgc tgccacctga catcatcgac
    53281 ggagtagtag gaaattccga gtatttcgaa ccggagaaga agctcggcat ccggcaactg
    53341 gcgtacgttt cgatgcaggg ggtggaagtt catttccccg accacttctc gtccgagtca
    53401 ggggagggga tcgtgcccgg ccggatcgag gccgcaaacc aggtgttgtc cttcggtgag
    53461 cactggcgaa gagccgggac gcggaaagga aaccagatcg gcgtattcgg cgccgcgtgg
    53521 gacgcctccg gcctgcacaa cgagacgttc tggctcggct ggtcagcggt cgcacagttc
    53581 ggttggaaac ggggcggagc ggcaacggac aggcacacag ccgacttcat gaaactgtac
    53641 tacgggccgc tgtccggagg gatgacagag gtataccgca cgatgcagcg ccaggcgcgc
    53701 gcttgggagc ggacatggga ccaggttccg tcgcgggccc gcggaccgag ctatgggaac
    53761 tcgacgggga aggggatcgg gacgcagcga cgggatatga cgctttcacc accggcgatt
    53821 ccgcgaatgc cggaccttca agtgacaccg cacatcggcg gcaagtatgc tgcctttctc
    53881 acggaggccc gcgcgcagat gcccgagaac gaggcgttga agctggaact ttacgctcag
    53941 ttcggcctgg ccgacaggaa ccgctacaac ctggaggtat tcctttcgat cgcggagttg
    54001 gttgggcatc actggcggct cctgactgaa atgactgggg ccgagcaaga ccttgtgcgt
    54061 gcgtacgccg ctgcgcagaa gagccagcac cggcgggcag tgggccagtt agtagccgcc
    54121 tacaaccgga tcgaccggat cgaaaggcaa cgtgagcgga ccctgcacga gttgcagcaa
    54181 gtctgggaga agggccgata tccgcgcggc cagagcgtgg gcggacggag cttcgtagat
    54241 ctcaacgacg atacgaagga ctact
//
LOCUS       DATIFQ010000187        28333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1403853, whole genome shotgun sequence.
ACCESSION   DATIFQ010000187 DATIFQ010000000
VERSION     DATIFQ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 28333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28333
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403853"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VLE22_08495"
     CDS             complement(<1..1117)
                     /locus_tag="VLE22_08495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB14485.1"
                     /translation="MTRPEDPSRRLLALSVALVLCNFLAGLSFIPYPGIQNDEAVFAW
                     GVYDPGYAISQLSVFKYRIPMMLISYLGALKSWAYAPILAVWEASVWSVRLPVLAIGA
                     LTIWLLCWLVYRIAGARAALAGCALLAFDTTYLLTTVYDWGPVALQHLLMVGGVLSLV
                     RFHQGASERSLAVAFLLFGLALWDKTLFVWTLVAIVVAGVLLFWRPIRASVNRKTVVL
                     ALISFSIGAFPLLRYNVQKRGETWRRNVAWSRDELASKTQVLRSSLDGSGLFGYIAVD
                     EPTANSREPRTPIERASVALSEFAGHPQKGPLPYLVIAAILLLPFLWRTPARKPALFA
                     VITTMLVWVQMAFTKGAGAAVHHTALLWPWPHMLVGVA"
     gene            1512..1895
                     /locus_tag="VLE22_08500"
     CDS             1512..1895
                     /locus_tag="VLE22_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSB14486.1"
                     /translation="MTKSELNRFKQILENRQAELAKVLRNREGIAIEKSPDALDEVQH
                     AAERELAIRNLDRESHMLRNVKLALRRIADGTFGTCLNCEEPINPKRVEAVPWSAFCI
                     RCQEMADRQAAQGDESLEDLLMNAA"
     gene            complement(1948..2616)
                     /gene="pgeF"
                     /locus_tag="VLE22_08505"
     CDS             complement(1948..2616)
                     /gene="pgeF"
                     /locus_tag="VLE22_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HSB14487.1"
                     /translation="MFHRDEHGIYHVPAFDVLGWIEHGFGSRHATLWRDDPRVVALHQ
                     IHSAICLVADGRPGRIGEGDALVTAVPGALLTVRTADCIPALLADERRHVVAAVHAGW
                     RGTLQDVVGAAIRSMRERFCSRPEDLLTAIGPGIGACCYEVGPEVAVQFKELFPERDD
                     LDGTTKLDLVEANRRRLLAAGVPSARISVAGLCTYCRPEEFHSYRRDRAGAGRMASAI
                     GIRP"
     gene            complement(2616..3182)
                     /locus_tag="VLE22_08510"
     CDS             complement(2616..3182)
                     /locus_tag="VLE22_08510"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSB14488.1"
                     /translation="MSFLQNQFEKNVLVTSVDYVFNWARQSSIWPLTFGLACCAIEMI
                     ASSAARFDIARFGAEVFRPSPRQADLMIVSGTVTLKMAPVLKRLHDQMPDPKWVISMG
                     ACSSVGGPFNTYAVLQGVDKVVPVDVYVAGCPPRPENLFYALLKLQDKIDRMTTLVRR
                     PTEVRLDESMLENFKKQVMIAQVKNPAA"
     gene            complement(3231..4169)
                     /locus_tag="VLE22_08515"
     CDS             complement(3231..4169)
                     /locus_tag="VLE22_08515"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB14489.1"
                     /translation="MNSYIVTGGAGFIGSSLVRALLGRGAERVTVIDNFFSGRERNLE
                     EVRDRVELHRADIRSYEKIAPLFRGAGVVFHLAAIPSVPRSISEPVPSHQVNIDGTFN
                     VLRACSEAGVGRVVYAASSSAYGGSDVLPKVETMSPDPKSPYAAQKLAGEYYAHVFAS
                     CFELETVSLRYFNVYGPRQDPSSPYSGVISVFMKCLLARVPPRIFGDGEQSRDFTYVE
                     DVAALTVKAARAPGVSGRLYNGGSGGRYTLNQVWKILQEIEGVEIPARHGPPRAGDVR
                     HSQADTAAAARDLGHAPSFTVEEGLRRTLAWYRENR"
     gene            complement(4198..5037)
                     /locus_tag="VLE22_08520"
     CDS             complement(4198..5037)
                     /locus_tag="VLE22_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB14490.1"
                     /translation="MPYPFRHAICNEVFEKWKFADTCKAIRQAGYSGIEIAPFTLAED
                     PAAITGAQRREYRAVMESEGLAFVGLHWLMVAPKGLHVTTPDKALREKSWLHVRHLVD
                     LCAGLGPDGVMIFGSPKQRNALGGLSRAQATRHFVDGLAAIAPHAAERGVTILVEALP
                     PKDTDVVTSLDEAAAIVREINHPAIRTMFDTHNAIAEVEPHASLVDRYFDLIRHLHVN
                     EMDGRHPGTGDYDFKSVLKVFARRGYQQWVSLEVFDFSAGAEKIASDSLRFLQGEIKR
                     LGV"
     gene            complement(5154..8246)
                     /locus_tag="VLE22_08525"
     CDS             complement(5154..8246)
                     /locus_tag="VLE22_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB14491.1"
                     /translation="MNFSELFIRRPVATSLLMLAIALFGFVAYRGLPVSDLPNVDFPT
                     ILVSAGLSGANPDTMASAVATPLEKEFSTIEGLDSMTSSSGSGSTSITLQFALSRSLD
                     AAAQDVQSAITRATRLLPPGMPNPPTFRKVNPADQPILYMALTSDTMPLYDLDEYAET
                     LMAQRISMVSGVAQVSVFGAAKYAVRIRIDPEALAVKGIGIDEVTEVVQRWNVNMPTG
                     ELYGPNQAYTIQATGQLTKAELYRPMVIAYRNGSPVRLRDIADVVDSIEEDKVASWYN
                     DQSGSRRAIVLAVQRQPGQNTIAVADSVKALMPVFQAQLPPSVRLNILYDRSATIRES
                     FEDVQFTMVLTLGLVVMVIFLFLRNVSATIIPSLALPFSIVGTFAVMYLLGYSLNNLS
                     MMALILSVGYVVDDAIVMLENIVRHMEHGEAPLQAALSGSREIGFTIVSMTLSLAAVF
                     IPVLFLGGIVGRLFREFAVTITAAILISGLVSVTLTPMLSSRFLRPPSEERHGWFYRL
                     TESIFQGMLSLYGRTLRWTLKFRPVTLAISFLILAATVWLFLKTPKGFIPSEDTDQIF
                     VVTEAVQGTSYPVMFRYQQQVAEILRTDPNVDQFMSSAGGFGGGGGLGGPNFGRMFLH
                     LKPRSERKLSADELMEYFRPKLSVVPGMRVFMQNPPTIRIGGQLTRSLYQFTLQGTDI
                     DELYRAAQKMEREMVAMPLLQDVNTDLLLKKPQVNVVIDRDKSASLKVTAEQIEDALY
                     SAYGARWISTIYAPNNQYRVIMESRPEFQDEPNALSLLHVRSGSGTLVPLDTVARLTE
                     SLGPQAINHSGQLPSVTISFNLKPGASLGDAVDQIQQAAERLLPATVTTTFQGAAQAF
                     QQAGNLWLLLIVAILVIYIVLGILYESYIHPITILSGLPSAGFGALLTLMVFRVELNI
                     YSFVGLIMLIGIVKKNAIMQIDFALAAERQEGKPPMEAIYQGCLIRFRPIMMTTMAAL
                     FGSVPIALGYGAGGEARQPLGLAVVGGLLFSQLVTLYLTPVYYTYLASLQSRLQRRVA
                     "
     gene            complement(8243..9427)
                     /locus_tag="VLE22_08530"
     CDS             complement(8243..9427)
                     /locus_tag="VLE22_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB14492.1"
                     /translation="MRHANLAIPVAVLLAGCSSSNVPKATPRAQAAMVTTALAAEKTM
                     PVDIRVVGTVEAYTTIRVKAQVGGELTKVQFREGDNVRKGDLLFVIDTRPFDEAILQA
                     KANLERDAAMLRQAEANLQRDLAQEKFAREQAARYEKLFREGVMSKEQTDQFRTEADV
                     RAQAVRADQAAIGSARAAMAADEAALSNAQLQRGYCMIRSPIGGRTGDLMVKQGNLVK
                     ANDVELVTINQIQPILVTFSVPERDLPAIKQRRASRKLTVLVNPAATPDQVDEGNLTF
                     IDNAVDTTTGAIKLKATFKNTGSRLWPGQFVNVVLRLDTVPDAVVVPTLAIQTSQTGE
                     YVYVVKPDMTAEMRPVITGLRVGQEIVIERGLKIGETVVTEGQLRLAPGMRVRTRQAQ
                     GA"
     gene            9512..11080
                     /locus_tag="VLE22_08535"
     CDS             9512..11080
                     /locus_tag="VLE22_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HSB14493.1"
                     /translation="MTSPHTLSRRGFLGVAAVTRMPASSPVTVRAFDASGAAAKEDLL
                     NTLLLMNADGRPFELLPTVKGDGMATIEVPAEKFEVMMVLPVRGFGQVYLYADNGGPL
                     YSAAHVPRGEFLLNYEFARSRAVFVRRYMRAAEAEGVSFSPEIERRLEDGEHALDRAA
                     KARDTVERVRHSNNSLAETMWAGEMAALERARHRIARGGRRPGFLFGCNAFSYAKSEE
                     YARRYNALLNFATLPFYRAQTEREERKPDYSRVEEILSKMAGTGTLTKGHPLVWFHRA
                     GVPAFLKDKSWGDLKRSCRDYILGSVSRFRTRIHTWDVINEAHDWANDLGLDAEQLVE
                     ITRLASESAREADPTAFRVVNSCCTWGEYAARRRSYSGPLGRAARTPFEYVRALDDAK
                     VPYEAVGLQVYYPSRDMLEIEKHLERFFRFGKPIHITELGVSSSGEPVPSGEVRTPSR
                     NVWHGESWSEAIQADWVEQFYTICYSKPEIQAITWWDFADPSFIPHGGFLDKDFRPKA
                     AYERLAKLITGWRS"
     gene            complement(11142..13364)
                     /locus_tag="VLE22_08540"
     CDS             complement(11142..13364)
                     /locus_tag="VLE22_08540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB14494.1"
                     /translation="MLSLALILFGAAFTLVSAYALGCVILRSLPVPRVIVFGVGSAAL
                     SLVVFALLAAGLGGHRVFLPLGLCLIAAALLSDRGTAFSLWSPSSIVFSLWNLRGRGF
                     RLWTPWSLFVPILSVYSILYFVHALAPEIQPDAITYHLGLVAEYTRLGGFPHRVAFYE
                     VLPQGMEMLFTFAFAFGKHSSAKLVHFAFLAATFPLMLSIGRRLGWPTWVSASAAALY
                     VISPVAGVTGTSAYNDAALVFFSLAVFYVLWLWKEESEVRYLFPAGILAGFCYSVKMT
                     GLLMAVASVLFVAVMARRRARALLLVTGGATLMIAPWMTRNTVVCGNPVAPFFNRGFP
                     NEHFHIASETELLKQLRTYKGVTAAEAPLELTVRGELLRGLLGPVFLLAPLGLFALRK
                     RPGRVAAVMAVLMAVPWFLNTGTRFLLPALPWLAFLIAAALPRPLVLACVVLHAVTAF
                     PAVITQYEAPSAWRLRGFPFAAALRIEPEDEYLRNNLWAYRLAELIEKNTLPGARVLV
                     LLNAPSAYVTRELVSYWQSSLGDRLTDALALAASQERGSLEQMEAGWPEQPVLALRFR
                     RVDGPPGNWGIHEVRLNRGWEVVRSSPRWELEASPNVWEAPLAFDGSIATRWALWEPA
                     RPGMYLTVDFGRPQPLTDVTIVTTRLAGGTKVEIEGMRPDGAWIMLGGSPTVTLYPPV
                     NLRPLAVRAVRRQGVRYILARIGNSGHGLTGRALASRPLDWGLERVASFEEVTLYRIP
                     "
     gene            complement(13369..14385)
                     /locus_tag="VLE22_08545"
     CDS             complement(13369..14385)
                     /locus_tag="VLE22_08545"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSB14495.1"
                     /translation="MSAKRALITGITGQDGSYLAELLLAKGYEVHGVVRRVALEDPEH
                     RLSRIQHIARQLHLHAGSLESYASIHQVIEKARPEECYHLAAQSFVSYSFDDEFSTLN
                     ANINGTHFLLAALKNLIRDCRFYFAGSSEMFGSAEEVPQNERTRFHPRSTYGISKVAG
                     FELTRNYREAYSMHCSNGILFNHESPRRGFEFVTRKITSGVARIRAGKANELRLGNLE
                     AKRDWGHAREYVEAMWLMLQQPEPDDYVVATGETHSVREFAELAFAQAGLDYRDYVKP
                     DPELFRPAEVNVLIGDASKARKTLGWEHRVGFEQLVREMVDTDCRNLGLALDHRQQTP
                     IAVD"
     gene            complement(14385..15785)
                     /locus_tag="VLE22_08550"
     CDS             complement(14385..15785)
                     /locus_tag="VLE22_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSB14496.1"
                     /translation="MRPTLVGAWLAVSAVTISAQTGIEGTWQGSLSAGGATLRLQLNI
                     KKDAKGALTVTLDSLDQGAMGLRATEVSLKLGALHFNVPSVGGSYDGKLDEKGNGING
                     TWSQGGFSAPLDFERAGKPRTLPRPQEPTRPFPYDEQEATFPNQAAGITLAGTLTLPR
                     GKGPFPAALLISGSGPQDRDESIMGHRPFLVLADRLTREGLAVLRFDDRGTGKSTGNF
                     AVATTLDFAADAEAGLAWLRTRAEIDPKRIGLIGHSEGANVAAIVASRAPAAFVVMMA
                     PSGVRGEDLLVSQTAAIMNALGLPLDQVEKTLAFNKRAYAIARQEKDAAVIERKLRSL
                     LPELSGNQQIPKDMIEGQIKMLSSPWFRHFIDYDPVPAMKSVRSPVLAMFGEKDVQVP
                     ADENLKAIRDALQSGGNKDFATIKLPGLNHLFQTAGTGLPMEYGQIEETIAPTALDLI
                     SKWLAERLKQLVLPSP"
     gene            complement(15828..16511)
                     /locus_tag="VLE22_08555"
     CDS             complement(15828..16511)
                     /locus_tag="VLE22_08555"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HSB14497.1"
                     /translation="MKYLSGIMLVSFLAASLSGAALPKNQAYGEYVEARTADVYTGPC
                     FANGEVGFGGDMAVFGWKVQKGNWQGVTLDGLSVVAAIRASHTLGDPTRTAYPVKSIL
                     IVDERAGLEQRQALKSFAQRLAGDLLQDIVRVEYRPIDFKFDDDNVHSMKALVTAGEL
                     ARIQTRPIGEGDHICHNEDVYYSPLTKLDHAMPAVASAHTFRGKGLNANWSSPDKRSA
                     FVGTFNFQE"
     gene            complement(16600..16815)
                     /locus_tag="VLE22_08560"
     CDS             complement(16600..16815)
                     /locus_tag="VLE22_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14498.1"
                     /translation="MAGSAFNVMQEERCANIRWKGLYVDPEADSALQRGDERIFWCVK
                     TQLNLGPDGKLVDNYECNPCRDCYKAL"
     gene            complement(16818..17792)
                     /locus_tag="VLE22_08565"
     CDS             complement(16818..17792)
                     /locus_tag="VLE22_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HSB14499.1"
                     /translation="MQSFEYASPATLKEALALLGNQWGEADVLAGGTDQLSLMKEYVH
                     TPKRVVNIKGIKELGGITKTAQGLRIGATVTLDQLLASSAVRAEYPSLITAAQGVTSP
                     QIRNMGTVGGDLCQRPRCWYFRMGMGLLAKGGSLVREGDNRYHAIFSGGPAYFVSASS
                     FGPALVALGAKVKLASASGTRELEVEKFFVMPRIDTARETALQPSEILTEIIVPSAVG
                     VRNATYEVRQKEALDWPLATASVALKMKGAAVASARVVMGHVAPTPWAALQADQLLVG
                     KTIDEGVAEEAAQAAVSGAQPLSGNGYKVQIARTAVKRALLAAARRRG"
     gene            complement(17792..19939)
                     /locus_tag="VLE22_08570"
     CDS             complement(17792..19939)
                     /locus_tag="VLE22_08570"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSB14500.1"
                     /translation="MPDPNYSWPPLGKSRVMRKRMSRLDGPAKASGKAKYGYDIHTPD
                     LLFGALLTSPYAHARVRSIDTGAAEKMTGVMAVEVVSPAGTEIQWAGTEVAAVAATSE
                     TIAADAVRAIKADYEVLPHLVNEEDLGKTGSRSKPAGEILTGDPDQAFKDADTVSEGY
                     YGCPVLTHCTQESHGQVIAWKGDKIEFWPSTQNLSGIGGDLARALEVPAANIHAHQDH
                     MGGGFGSKFAADRWGMICAKLSKKAGGRAVKLFLDRRADLEMAGNRPSIFAKVRLAAK
                     KDGTITAWQSESWGTSGVSGGGVSPTQLPYVYVQIPNKRVNHTGVVTNNGGARAWRAP
                     NHPQAAFVTCCAIDDMAAAVKMDPLEVFKKNADYTARAEVYRAQLDKAAELAEWKKLW
                     HPRDQAGSGPMRRGLGIGVGTWGGLGHPSDCRTRIHPDGSVDVELGSQDLGTGTRTVI
                     AMVAAESLGLTVPQINVKIGDSSYPQSGASGGSTTVGGVSVSTRKATLNALAKVYEAV
                     APALGAPADQLEAVDGKIQVKGNPAKSMTWKAACQKIGVKPIEAMGTNNPKQPMGLIS
                     QGVGGAQIADVSVDIETGVVKLNRLVAVQDCGLIINPKTAESQVYGACIMSICGALYE
                     ERLMCEKTGRFLNADMEFYKLAGIADIGEIVVHLDINEEHDKRGVIGLGEPPVVPGMG
                     AIANAVANAIGVRVPRIPLTPDRVLEALARRTA"
     gene            complement(19932..20573)
                     /locus_tag="VLE22_08575"
     CDS             complement(19932..20573)
                     /locus_tag="VLE22_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSB14501.1"
                     /translation="MSLRKDPDVEKKPKTGFSRRGFIRGVGLGSGAVGILQTEAQAEP
                     VAAADVAGPGPVPITLTINGKPVKLSVEPRVTLLDALRDHLDMTGAKKVCDRGTCGSC
                     TVIMAGKVVYACSVLAIDAQGKPIQTIEGLATAGRLHPVEAAFVNNDAQQCGFCTPGF
                     VMAAKGFLDHNPDPTMDQVKAGLGGNLCRCGTYMGVRKAVLEAAKQMKGAASA"
     gene            21031..22053
                     /locus_tag="VLE22_08580"
     CDS             21031..22053
                     /locus_tag="VLE22_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSB14502.1"
                     /translation="MITNDSGYLRDLLDQPRALRDTIAALNVKPALAALAARRFERIV
                     LTGMGSSLHALHPLNQRLAAGGFCPVMVETSELLHGLRGLLTGETLLVAVSQSGRSAE
                     TVRLLEQEGPRPHTIGVTNTPDSPLALVPDAAVVTRAGAENSVACKTYLSILAALDWL
                     GSVFCGDDLEAMLPELAQASMLVDNYLTRWQDHVEELHSELAGVEHVFLAGRGCSLAT
                     VGNGGLILKESAHVHAEGMSAPAFRHGPIDMLRDDVFVLVFTGEPATAPLNVRLFDDI
                     RNAGGRTALISETTAGAFRLPVGPARLRPILEMLPVQMMSLALAQLKDHEPGVFRHTS
                     KVTTVE"
     gene            complement(22076..24019)
                     /locus_tag="VLE22_08585"
     CDS             complement(22076..24019)
                     /locus_tag="VLE22_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB14503.1"
                     /translation="MKRVTAILCVLLMVAPLSGQTGASLNNSSQASGGFFGGFTRAYK
                     YREEAPINLANSSRIDNLLRAGRIYLSLQDAIALALENNLDIALSRYGPRAADADILR
                     AQAGGLLRGIPTSITSGPSSALAQATGTAGGGGGGGGTGGGGGTTSTGAAIITTTGTA
                     LPNLDPVISTQYSWTHRTVPQTNSFTTGTNSLVIKNQSGNMSFRQGFLTGTNLEFDWI
                     NSPAETNSGRSDFNPYISGSLNLTITQRLLQGFGTAVNNRNIRIARNDRRVSDLVFKQ
                     QVITTVSAVVALYWDLVSFNEDVKVKQQALALAEKLYNDNKKQVEIGTLAPIEIVRAE
                     AEVARSQQELTVSETRVLQQETIIKDALSRTGVASPVVADARIVPTDQMRMPDSEAIE
                     PIQDLVNRALDSRPEIAQTRLLVENTKIGLQGTKSALRPSLDLFGYAQNNGLSGSINS
                     LPVPPFPGQPAGTVTPRDPNSVDPYFIGGYGKVLSQIFRRNFPDYGIGIQLNIPLRNR
                     AAQADMIRDQLSLRQQEIRQQQLVNQVRVDVTNSLIAVQQARAGYQAAMKARVLQEQT
                     LDAEEKKYALGASTIFFVIQAQRDLAQARSAEVTALSTYSKARNELDRSTGQTLGVYN
                     IAIDEAMAGRVSRAPSAIPEVKN"
     gene            24124..24717
                     /gene="pyrE"
                     /locus_tag="VLE22_08590"
     CDS             24124..24717
                     /gene="pyrE"
                     /locus_tag="VLE22_08590"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSB14504.1"
                     /translation="MNLQAEEVLKVFNETGAYLRGHFRLTSGLHSPEYLQCALVLQHP
                     RHAERLGQALADRLGEILAGARVDVVASPAIGGLIIGHEAARALGARFIFAERDAEKK
                     MVLRRGFTVQPGETAVVVEDVVTTGGSTREVVETLQAAGARVLAAGSIIDRSGGQADV
                     SVPRVALATLQVVTWQPEACPLCRDGSPVTKPGSRPV"
     gene            24725..25468
                     /gene="truA"
                     /locus_tag="VLE22_08595"
     CDS             24725..25468
                     /gene="truA"
                     /locus_tag="VLE22_08595"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HSB14505.1"
                     /translation="MRRIRVTVAYDGTGYHGWQVQPGLPTIQGVLERVAEEIEGKPIK
                     VHGSGRTDAGVHALGQVAAFDFENPIPTPNLRRAMNRLLPPEIRVLDAEEAPADFHPR
                     HRAVAKTYEYRIFRGEICPPFDRRYAHHYPYPMAVDLFVEAAPLFEGEHDFSAFAAAD
                     EKDALGASKVRTIFSSSAEIVGEWMFYRVRGSGFLKHMVRNIVGVLLEAGKGNLGEDE
                     IRGYFVPGYPGKAGPTVPACGLFLVSVEY"
     gene            complement(25515..26741)
                     /locus_tag="VLE22_08600"
     CDS             complement(25515..26741)
                     /locus_tag="VLE22_08600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB14506.1"
                     /translation="MPRLRWLMISFAFLATVINYLDRQTLSVAAPVLREEFAMSAGDY
                     SRVVFAFLLAYTILNGVSGALLDRLGTRVGYAICMAWWSAAAALHTLATGAWSLGAFR
                     FLLGMGEAGNWPAAVKVVAEWFPERERALASGIFNSGSAVGAILAPPLVAWLILQFGW
                     RTSFLAVAVLGFLWLAAWWPVYRSPGLTQPESRAAASGTSFSLLDLFRTRFVWSFTLS
                     KVFIDPVWYFYIFWFPEYLKTARGFDMASIGRYAWIPFAVAGLGNLIGGWLSGWLIGL
                     GLPVMNARRTAVTIFALLVTSAIPAVLVTNMRVAMGLVSLAMLGYTGASASMLAMPAD
                     VFPKNAVASVYGLASMGSGFGGMLFTLLTGWAVDHFSYTPVFIGFGILPLICAATLWA
                     LLSASTSPEGDRPTLL"
     gene            complement(26755..27654)
                     /locus_tag="VLE22_08605"
     CDS             complement(26755..27654)
                     /locus_tag="VLE22_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14507.1"
                     /translation="MDLSRREALLTALGGTAAAAAAKSPGVPTVPFGKHRVSRLIVGG
                     NPVSGTSHLNNQLSREMVDYFTSANIKRMLRDCENAGINSWQSRGDKHIIRMLHEYRQ
                     EGGTIQWIGQTATEIVFDRNLREMAGEAPIGIYHHGSLTDRLWAAGKIDDARDNLKRI
                     RDTGTRVGLGTHIPQVIDYVESKGWDVDFYMTCVYNISRTKEEVSRLAGRPVDDELFW
                     DPDREEMLKRVRQTSRQCLIFKVYGATRHCDTRERMLSALRLAFQYAKPDDAVVIGMF
                     PKHKDQVRENCQLVNEAIHGAAS"
     gene            complement(27790..>28333)
                     /locus_tag="VLE22_08610"
     CDS             complement(27790..>28333)
                     /locus_tag="VLE22_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14508.1"
                     /translation="TAGKLLTDNFSTLYPASNYFQVGSTYSLAADGAGKIQNFLPQTG
                     TSGKLTSNYTITNSGSQTTSAGKLGGEVVALKLNVDASANNLLTHPASLPFGDLYYCP
                     SGSDDLKGKKISEILTTAAQVMGGALASPPSGYTYATLDQLLQNLNESFKSGVTSSVN
                     AGCQVSAWANTNLKKTSCAP"
BASE COUNT         5352 a         8389 c         9409 g         5183 t
ORIGIN      
        1 aggcgacgcc gacgagcata tgcggccagg gccaaaggag cgcggtatgg tgcaccgctg
       61 cgccggcgcc tttcgtgaag gccatctgca cccacacgag catggtcgtg atcacggcga
      121 acaacgccgg tttgcgcgcc ggcgtgcgcc acagaaacgg cagaagaagg attgcggcga
      181 ttacgaggta tggcagcggg cctttctgcg ggtggccggc gaactcgctc agcgcaacgg
      241 aagcgcgctc gatcggtgtg cgcggctcgc gcgagttcgc cgtgggctca tcgacggcaa
      301 tgtagccgaa cagtccggag ccgtcgaggc tgctgcgcag tacttgcgtc ttactggcta
      361 actcgtcccg ggaccaggcg acgttccgcc gccaggtctc gcctcgtttc tgtacgttgt
      421 atcgcaagag tggaaaggct ccgattgaga acgaaatcag ggccagcacc acggttttcc
      481 ggttgacgga agctcggatc ggccgccaga acagcagcac gcctgcgaca acgattgcta
      541 ccagcgtcca gacgaatagc gtcttgtccc atagggccag accgaaaaga aggaaagcca
      601 ccgcgagcga acgctcggaa gctccctggt gaaatcgaac aagagagagc acgccaccga
      661 ccatcaaaag gtgctggagg gcgactgggc cccagtcgta aaccgtcgtg agcaggtagg
      721 tcgtgtcgaa ggcgagtagg gcacagccgg ccagcgccgc tcgcgcaccc gcgatgcggt
      781 acacgagcca acagagtagc cagatggtca gcgcacctat tgccaaaacc ggcaaccgga
      841 ccgaccagac tgacgcctcc caaaccgcca ggatcggagc gtaagcccaa gatttcaaag
      901 cacccaagta gctgatcagc atcattggga tacggtactt gaacacgctg agttgggaga
      961 ttgcgtagcc ggggtcgtaa acaccccagg caaacacagc ttcgtcgttc tggataccag
     1021 gatatggaat gaatgacagg ccggcgagga aattgcacag caccaaggcc acagagagcg
     1081 caagcagtcg tcttgagggg tcttctggcc gggtcaagtc cccgatcata gcatgacccc
     1141 cacttgacac ggtgtgctat gctgatctct tttgtcaata acgagttaaa tctgcacgtt
     1201 tcggtgaaac ttccgcgcaa aatttgcgtc aaagagtaat cggggcgagc tgatgctgaa
     1261 gcgacccaat ttggaactcc agatgctcgg gacgatcctc gtcggtgacg accgtcgcga
     1321 agccagatcg aacgggagga gaacaaaagg ggaagtttta gcggaatgtg ctaatattta
     1381 ctgaaacgcc cggcttcaca tttacgtcta taggttttca ggaggtgagt gaagcttcca
     1441 aacggcacag taagccagca acccaaaagg ttgcggtaag aacagccgat ggagaacgga
     1501 ggaatgaggc catgacgaag agcgaattga accgattcaa gcaaatcctg gagaacaggc
     1561 aggcggaact cgccaaagtg ctgaggaatc gtgagggcat cgccattgag aagagccctg
     1621 atgcgctcga cgaggtgcaa cacgcggcgg aacgggaact cgccatccgg aaccttgacc
     1681 gcgaatcgca catgttgcgc aacgtcaaac ttgcgcttcg ccggattgcc gacggtacgt
     1741 tcggcacttg cctcaactgc gaggagccca tcaacccgaa gcgagtcgag gcagtgccgt
     1801 ggtcggcgtt ttgcatccgt tgccaggaga tggcggaccg ccaggcggcg cagggagatg
     1861 agagtctcga agacctcctg atgaacgcgg cgtaagaacc tcttgtgaat tatcgggcgc
     1921 gggttcaacc ccgcgccctt cgtgtcttca gggacggatg ccgatggccg aagccattcg
     1981 tccggcgcca gcgcgatccc ggcggtacga atgaaactcc tccggacggc agtaggtgca
     2041 cagaccggcg acggagatac gcgccgacgg aacaccagcc gcgagtagcc gccggcgatt
     2101 cgcctccacc agatcgagct tcgtggttcc gtccaggtca tccctctcgg ggaacagctc
     2161 cttgaattgc accgccacct ccggaccgac ctcgtaacag catgctccga tgcccggacc
     2221 gatggcggtt agcagatcct ccggccggga gcagaaccgc tcacgcatcg agcggatcgc
     2281 cgcgcccaca acgtcctgaa gcgtgccgcg ccacccggcg tggaccgccg cgacgacgtg
     2341 ccggcgctcg tcggccagga gcgccggaat gcagtcggcg gttcgtacag tgagcagggc
     2401 tcccggcacc gctgtcacga gcgcgtcgcc ttcaccgatg cggccggggc ggccatcggc
     2461 gaccaagcag atggctgagt gaatctggtg cagcgccacc acgcgcggat cgtcccgcca
     2521 gagggttgcg tgacgggaac cgaagccgtg ctcgatccag ccaagtacgt cgaacgccgg
     2581 gacgtggtag attccgtgtt cgtcgcgatg gaacactagg cggcggggtt cttgacctgg
     2641 gcgatcatga cctgcttctt gaaattctcg agcatggact cgtcgagccg gacctcggtg
     2701 gggcggcgga cgagggtggt catgcggtcg atcttgtcct gaagcttgag cagcgcgtag
     2761 aaaagattct ctgggcgcgg cgggcagcca gccacataga cgtccacggg gacgaccttg
     2821 tcaacgccct ggagcacggc gtacgtattg aacgggccgc cgacactgga gcaggcgccc
     2881 atcgagatta cccatttcgg gtcgggcatc tggtcatgaa ggcgcttgag cactggcgcc
     2941 atcttgagcg tcacggtgcc ggagacgatc atcaggtcgg cctggcgcgg gctcgggcgg
     3001 aagacttcgg ctccgaaacg ggcaatgtcg aagcgcgccg cgctggaagc gatcatttcg
     3061 atggcgcagc acgccagtcc gaaggtgagc ggccagatag aagactgacg cgcccagttg
     3121 aacacgtagt ccaccgacgt gaccagcacg tttttctcaa actggttctg caaaaaagac
     3181 atcagcttga cgcctccgtc acaaccccat tctatcatcg cctggccggc tcaccggttt
     3241 tcgcggtacc acgccagtgt acggcgaagc ccctcctcga cggtgaagga cggcgcgtgg
     3301 ccgaggtctc gcgcggcggc ggcggtgtcc gcctgggagt ggcggacatc tccggcgcgg
     3361 ggagggccgt gacgggcggg aatctcgact ccttcgatct cctggaggat cttccagacc
     3421 tgattcagcg tgtagcggcc gccgctgccg ccgttgtaga ggcgtccgga gactccgggc
     3481 gcgcgcgccg cctttactgt gagcgcggcg acgtcctcga cgtaagtgaa gtcccgggac
     3541 tgctcgccgt cgccgaaaat cctgggcggg acgcgagcaa gcaggcactt catgaagacc
     3601 gagatcacgc cggagtacgg gctggacggg tcctggcgcg ggccgtacac gttgaagtaa
     3661 cgcaaggaga ccgtctctag ctcgaaacac gaggcgaaga catgtgcata gtactctccc
     3721 gcgagtttct gcgcggcgta cggcgacttc ggatcgggcg acatggtttc cactttcggc
     3781 agcacatcgc tgccgccata tgcagaggag gacgcagcgt agacgacgcg gccgacgccc
     3841 gcctccgagc aggcgcggag gacgttgaac gtaccgtcaa tattgacttg atgagaaggg
     3901 acgggctctg aaatggatcg cgggaccgac gggatggcag cgaggtggaa gacaacgccg
     3961 gcgccgcgga agagcggcgc gatcttctcg tagctgcgaa tgtcggcgcg gtgcagttcg
     4021 acccggtcgc ggacttcctc gaggttccgc tcccggccgc tgaaaaagtt gtcgataacg
     4081 gtgactctct ccgcgccccg gccgagcagg gcacggacga gagaagatcc gataaatccg
     4141 gcgccgccgg tgacgatata gctgttcatg cgttcaaccg agagagtgcg cggggagtca
     4201 gacgccgagc ctcttgatct ctccctgtaa gaatcgtaac gaatcggagg cgatcttctc
     4261 cgcgccggcg gagaaatcga agacctcgag cgagacccat tgctggtagc cgcggcgggc
     4321 gaaaaccttg agaacggact tgaagtcgta gtccccggtg ccggggtgcc ggccgtccat
     4381 ctcgttgacg tgcaggtgac ggatcagatc gaagtagcga tcgacaaggg aagcgtgcgg
     4441 ttcgacttcc gcgatggcat tgtgggtgtc gaacatggtg cggatggcgg ggtggttgat
     4501 ctcgcgtacg atcgcagcgg cctcgtcgag cgaggtgacg acatcggtgt ccttgggagg
     4561 gagggcttcg acgaggatcg tgacgccccg ctcggccgcg tgaggggcaa tggcggcgag
     4621 accgtcgacg aagtggcggg tggcctgggc gcggctcaag ccgccgaggg cgttgcgctg
     4681 cttgggggag ccaaagatca tgacaccgtc cggacccagg ccggcgcaga ggtcgacgag
     4741 atggcggaca tggagccagc ttttctcgcg gagcgccttg tccggcgtgg tgacgtggag
     4801 gcccttgggg gcgaccatga gccaatggag accgacgaag gcaagtcctt cggattccat
     4861 gaccgcgcgg tattcgcggc gctgagcgcc ggtgattgcc gcggggtcct cggcgagggt
     4921 gaacggagcg atctcgatgc cgctgtagcc tgcctggcgg atggccttgc aggtgtcggc
     4981 gaacttccat ttctcgaaga cttcgttgca gatggcgtgt cgaaagggat acggcatatc
     5041 gaggtactta gtgtatcgcg gcccgcggtc tgtggcgcgc gccgcgtgac caaacaaagc
     5101 cagatgggtg tgggtagagc gatagaaatc ggtctcaaag accgggcagc gatctacgcc
     5161 acgcggcgct ggaggcggga ctggagcgag gcgaggtagg tgtagtagac gggcgtcagg
     5221 taaagggtga ccaactgcga gaacaggagg ccaccaacca cggcgaggcc gagcggctgg
     5281 cgagcctcgc cgccggcgcc atagccgagg gcgatcggga ccgagccgaa cagcgcagcc
     5341 atggtggtca tcatgatcgg gcggaaacgg atgaggcagc cctgatagat ggcctccatg
     5401 ggcggcttgc cttcctgtct ttccgccgcc agcgcgaagt cgatctgcat aatggcgttc
     5461 ttcttcacga tgccgatcag cataatgagg ccgacgaacg agtaaatgtt caattcgacg
     5521 cggaacacca tcagcgtcaa cagcgcgccg aaaccggccg agggaaggcc cgagaggatc
     5581 gtgatcgggt ggatgtaact ttcgtagaga attcccagca cgatgtagat gaccaggatg
     5641 gccacgatca ggagcagcca caagttaccg gcctgctgga acgcttgggc ggcgccttga
     5701 aacgtcgtgg tgacggtcgc gggcaaaagc ctttcggccg cctgctggat ctgatcgacg
     5761 gcgtcgccga gcgaggcgcc gggcttcagg ttgaacgaga tggtgacgga ggggagctga
     5821 ccggagtggt tgatcgcctg ggggccgagg ctctcggtca ggcgggcgac ggtgtcgagc
     5881 ggcacgagtg tcccgctccc ggagcgcacg tggaggagcg acagcgcgtt cggttcgtcc
     5941 tggaattccg gccggctctc catgatcacc cggtactggt tgttcggcgc ataaatcgtc
     6001 gagatccagc gcgcgccgta cgcactgtac agcgcgtctt cgatctgctc cgccgtgacc
     6061 tttagcgagg cggacttatc gcggtcgatg acgacgttga cctgcggctt cttgagcagc
     6121 aggtcggtgt tgacgtcctg gagcagcggc atggccacca tttcgcgctc cattttctgc
     6181 gcggcccggt acagttcgtc gatgtcggtg ccctgaagcg tgaactggta gaggctgcgg
     6241 gtcaactgac cgccaatccg gatggtcggc ggattctgca tgaacacacg cattccgggc
     6301 accacggaga gctttggacg aaagtactcc atcaactcgt ccgcgctgag tttgcgctcg
     6361 gaacggggct tcaagtgaag gaacatgcgt ccgaagttgg gtccgccgag tccgccgcca
     6421 ccgccgaagc cgccggcgct ggacatgaac tgatcgacgt tcgggtcggt acgcaggatc
     6481 tccgccacct gttgctggta gcggaacatc accgggtacg aggttccctg caccgcctcc
     6541 gttacaacga agatctggtc ggtgtcttcg ctcgggatga agcctttggg agttttcagg
     6601 aacaaccaca cggtggccgc gagaatcagg aacgagatgg cgagcgtgac cgggcggaac
     6661 ttcagggtcc aacgtaaagt gcgtccgtac aacgagagca ttccctggaa gatcgattcg
     6721 gtcaggcggt agaaccatcc atggcgctcc tcggagggag ggcgaaggaa gcggctgctg
     6781 agcatcgggg tcaacgtcac ggagacgagc ccggaaatca ggatcgccgc ggtaatggtg
     6841 accgcgaact cgcggaacag ccggcccacg atgcccccca aaaacagcac gggaatgaac
     6901 acagcggcca gcgagagcgt catcgagacg atggtgaatc cgatctcacg tgagccgctc
     6961 agtgcggcct gaagcggcgc ttcgccatgc tccatgtggc ggacgatgtt ttcgagcatg
     7021 acaatagcgt cgtccaccac atacccaacc gagaggatca gcgccatcat cgacaggttg
     7081 ttcagcgagt acccgagtag gtacatgacg gcgaaggtgc caacaatgga gaagggcagg
     7141 gcgaggctcg gaataatcgt cgccgagacg ttgcgtaaga acaggaagat gaccatcacc
     7201 accaggccga gcgtgagcac catcgtgaac tggacgtctt cgaacgactc ccggatggtg
     7261 gcggaccggt cgtagaggat attcaggcgg accgaaggcg ggagctgcgc ctggaacacc
     7321 ggcatcagcg ccttgaccga gtctgcgacg gcgatcgtat tctgaccagg ctggcgttgc
     7381 accgcgagca cgatcgccct gcggctgccg gactggtcgt tgtaccacga tgcgactttg
     7441 tcctcttcga tgctgtcgac tacgtccgcg atgtcgcgga gccggacagg cgagccgttg
     7501 cggtacgcaa tcaccatcgg gcgatacagt tcggctttgg tgagctggcc ggtagcctgg
     7561 atggtgtagg cctggttcgg gccgtacaac tcgcccgtcg gcatgttcac gttccagcgc
     7621 tgcacgactt cggtgacttc gtcgatgccg atgcccttga ccgccaaggc ctcggggtcg
     7681 atccggatgc gcacagcgta cttcgccgcg ccgaacaccg acacttgggc cacgccgctg
     7741 accatcgaga tccgctgcgc catgagggtc tcggcgtact catcgaggtc gtagagaggc
     7801 atcgtgtccg aggtgagggc catgtacaga atgggttggt cggccgggtt caccttgcgg
     7861 aaggtgggtg gattgggcat tcccggcggc agcaggcggg tggcgcgagt gattgccgac
     7921 tggacgtcct gggcggcggc atcgagactg cggctgaggg cgaactgaag cgtgatcgag
     7981 gtgcttcccg agccgctcga cgacgtcatc gagtcgaggc cctcgatggt ggagaactct
     8041 ttttcgaggg gcgtggcaac ggccgaggcc atcgtgtccg ggttcgcgcc agacagcccg
     8101 gcggacacca ggatggtcgg aaaatcgacg ttaggcaggt cgctgaccgg cagcccccgg
     8161 taggccacga agccgaacag ggcgatcgcc agcatgagca ggctggtggc gacgggacgg
     8221 cggatgaaga gctcggagaa gttcatgcgc cttgtgcctg ccgtgtccgc actctcatgc
     8281 cgggcgcgag gcggagctgt ccttccgtga cgaccgtttc gccgatctta agaccccgct
     8341 caatcacgat ctcctgcccg acgcgaagcc cggtgatgac ggggcgcatt tccgccgtca
     8401 tgtccggttt cacgacatag acgtactcgc cggtctggct tgtctgtatc gcgagtgtcg
     8461 gaaccaccac ggcgtccggg acagtgtcga ggcgaagaac gacattcaca aattgcccag
     8521 gccacagacg gctgcccgtg ttcttgaatg tggctttcag tttgatggct ccggtggtgg
     8581 tatcgacggc gttgtcgatg aaagtgaggt tgccttcgtc aacctggtcc ggcgtggcgg
     8641 cggggttgac cagcaccgtg agtttgcggg aggcgcggcg ctgcttgatg gccggcaggt
     8701 ccctctccgg aaccgaaaac gtgaccagga tcggctggat ctggttgatc gtgaccagtt
     8761 cgacgtcgtt cgccttgacc aggttgccct gcttgaccat gagatcgccg gtccggccgc
     8821 cgatcggcga gcgaatcatg cagtagcccc gctggagctg cgcgttggag agggcggcct
     8881 cgtcggcggc catggcggca cgcgcactcc cgatagccgc ctggtccgcg cggacggcct
     8941 gcgcccgaac gtcggcttcg gttctgaact gatccgtctg ctctttcgac attactcctt
     9001 cgcggaagag tttctcgtag cgggcggcct gttcgcgggc gaacttctcc tgggcgaggt
     9061 cacgctggag gttggcctcg gcctgccgca gcatggcggc gtcgcgctcc aggtttgctt
     9121 tggcctgaag gatcgcctcg tcgaagggac gcgtgtcgat gacgaagagc agatcaccct
     9181 tccgaacgtt gtcgccttcg cggaactgga ccttcgtgag ttcaccaccg acttgtgcct
     9241 tcactcggat ggttgtatag gcttcgacgg ttccgacgac ccggatgtcc acgggcatcg
     9301 tcttttctgc ggccaaggca gtggtgacca tcgccgcctg ggccctgggc gtcgctttgg
     9361 gaacattgct gctggaacag ccggcgagca gcaccgccac aggaattgcg agatttgcgt
     9421 gcctcatcgg tccctggaat tactggacac agtgtaacac cgtgatacgc attgaccgcg
     9481 cgccaaagcc ggaatacaat ccggaatcat catgacaagc ccacacacac tgagccggcg
     9541 cggtttcctc ggagtcgcag cggtgacacg gatgccggcg agttcgccag tcaccgtccg
     9601 cgccttcgac gcctctggcg ccgcagcgaa ggaggacctt ctgaacactc ttctgctgat
     9661 gaacgcggac gggcggccgt ttgaattgct cccgacagtg aagggagacg gaatggccac
     9721 gatcgaagtg ccggcggaga agttcgaggt catgatggtg ctgccggtgc gcgggttcgg
     9781 gcaggtctat ctgtatgcgg acaacggcgg gccgctctac tccgccgcgc atgtgccacg
     9841 cggtgaattc ctgctcaact acgagttcgc gcgcagccgg gcggtgttcg tgcgtcgcta
     9901 catgcgggcg gcggaggcgg agggcgtgtc gttcagtccg gagatcgagc ggcggctcga
     9961 agacggcgag catgcgctgg accgcgcggc gaaagcgcgc gatacggtgg agcgggtgcg
    10021 gcactcgaac aactcgctgg ctgagactat gtgggcgggc gaaatggcgg cgcttgaacg
    10081 ggcgcggcat cggatcgcac gtggaggccg gcggccggga ttcctgttcg gctgtaacgc
    10141 gttctcctat gcaaagtccg aggagtacgc gcggcgttac aatgcgctgc tgaacttcgc
    10201 cacactgcct ttctatcgtg cgcaaaccga gcgtgaggag aggaagcccg actactccag
    10261 ggtggaggag atcctctcaa agatggcggg aacggggact ctcacgaaag gccacccact
    10321 cgtgtggttc caccgcgcgg gcgtgccggc gtttctgaag gacaagtcgt ggggagattt
    10381 gaagcgcagt tgccgggact acattctggg aagcgtgtcg cgctttcgca cgcgtatcca
    10441 cacgtgggac gtgatcaacg aagcgcacga ctgggccaac gatctcgggt tggatgcgga
    10501 gcaactcgtg gagatcacgc ggctcgcatc ggagagcgcg cgagaggcgg acccgacggc
    10561 atttcgtgtg gtgaactcgt gctgcacgtg gggagaatac gcggcgcggc ggcgttcata
    10621 ttccggtccg ctgggacgtg cggctcgtac gccgttcgag tacgtgcggg cgcttgacga
    10681 cgcgaaggtt ccgtatgagg cagtcgggtt acaggtgtac tatccgagcc gcgacatgct
    10741 ggagatcgag aagcacctgg aacggttctt tcgctttgga aagccgattc acatcacgga
    10801 actcggagtt tcttcgtccg gcgagccggt tccctcggga gaggtgagga cgccgagcag
    10861 aaacgtgtgg cacggcgagt cgtggagcga ggcgatccaa gccgattggg tcgagcagtt
    10921 ctacacaatc tgttacagca aaccggagat ccaggcgatc acctggtggg atttcgccga
    10981 cccgtcattc atcccgcacg gcggctttct ggacaaggat tttcgtccga aagcggcgta
    11041 cgaaaggctg gcgaagctga tcacggggtg gagaagttaa gagaaccgaa aatcggaaat
    11101 gagtgtactg tcacgggttt acacgggttt acgggttttt gttacgggat gcggtagagg
    11161 gtgacctcct cgaaggaggc gacacgctcg aggccccagt cgagcggccg gctcgcgagt
    11221 gcgcggccgg tgagaccgtg gcccgagttg ccgatacggg cgaggatgta gcgcactccc
    11281 tggcggcgga cagcgcgcac cgcgagcggc ctgagattga caggaggata gagcgttacc
    11341 gtcgggctcc cgccaagcat gatccaggct ccatcaggcc gcatcccttc gatctcgacc
    11401 ttggttccgc ccgcgaggcg tgttgtcacg atcgtcacgt cagtcaacgg ctgcggccgg
    11461 ccgaagtcga ctgtgagata cattccgggg cgggccggct cccacagggc ccagcgcgtg
    11521 gcgatgctgc cgtcgaacgc gagcggcgcc tcccaaacgt tgggcgacgc ttccaattcc
    11581 caccgcgggc tggagcggac cacctcccac ccgcggttca ggcgcacctc atggatgccc
    11641 cagtttcccg gaggaccgtc caccctccgg aagcgcagag caaggaccgg ctgctcgggc
    11701 catcccgcct ccatctgctc gagcgatccg cgctcctgag aggcggccaa tgcgagagca
    11761 tcagtgaggc ggtcgccaag cgacgactgc cagtagctga ccagttcgcg ggtgacgtag
    11821 gcgctaggcg catttaagag aacgaggacg cgcgccccgg gaagggtgtt cttctcgatc
    11881 agctccgcca gccggtacgc ccaaagattg ttccgaagat actcgtcttc cggctcgatg
    11941 cgaagcgccg cggcgaatgg gaagccgcgg agccgccatg cgcttggggc ctcgtattga
    12001 gtgatgaccg ccggaaacgc ggtgaccgca tgaaggacga cgcaggcgag caccaaaggg
    12061 cgtgggaggg ccgcggcaat caagaatgct agccaaggca gcgcgggcag cagaaaacgc
    12121 gtaccggtgt tgaggaacca gggcaccgcc atgagcaccg ccatcacggc cgcaacacgt
    12181 cccggccgct tccggagggc gaacagtccg agcggcgcca agagaaagac aggacccagg
    12241 agccctcgga gcagctcgcc acggactgtg agttccagag gcgcttcagc ggcggtgacg
    12301 cctttgtaag tacgcagttg tttgagcagc tctgtttccg aggcgatgtg gaagtgttcg
    12361 ttcgggaacc cacggttaaa gaacggcgcc accggattgc cgcaaacgac cgtattgcgg
    12421 gtcatccacg gcgcgatcat cagcgtggct cccccggtga caagcagcaa agcgcgggcg
    12481 cgccgtctcg ccatcacggc gacgaacaat acggacgcca ccgccatcaa caagccggtc
    12541 atctttacgg agtaacagaa gccggcgaga attccggcgg ggaacagata gcgaacctca
    12601 ctttcctctt tccagagcca cagaacgtaa aacacagcga gggagaagaa gaccaacgcc
    12661 gcgtcgttgt aagcactcgt tccggtgact ccagccacgg gcgagatcac gtaaagcgcg
    12721 gcagccgagg cggagaccca tgtgggccag ccgagccgcc ggccgattga gagcatcagc
    12781 ggaaacgtcg ccgccaggaa cgcgaaatgg accagcttcg cggaggaatg ctttccgaag
    12841 gcgaacgcga aggtgaacag catctccatt ccctgcggca ggacttcata gaaggccacg
    12901 cgatgcggaa agccgccaag gcgagtgtac tcggcgacaa ggccgaggtg gtaggtgatg
    12961 gcgtcgggct ggatttccgg ggcaagtgcg tggacgaagt aaaggatgga gtagacggag
    13021 aggattggga caaacaggga ccagggagtc cagagcctga agcctcggcc acggaggttc
    13081 cacaggctaa agactatgct actgggactc cacaggctaa aggctgtgcc gcggtcactt
    13141 agcagagccg ccgcgatcag gcagaggcca agcggcagga acacgcggtg accgccgagt
    13201 ccggcggcga gcaaggcgaa gaccaccagg ctaagggcgg cggagccgac tccgaagacg
    13261 atgacgcgcg ggaccggcag agatcgaagg atgacgcagc ccagggcata agccgagacg
    13321 agggtgaacg cggcgccgaa gaggatcaga gcgagcgaga gcaagcgatc aatccacggc
    13381 gatcggagtc tgttgccggt ggtcgagcgc caggccgagg ttgcggcagt ccgtatcgac
    13441 catttcccga acgagttgct cgaagccgac ccgatgttcc catccaagcg tcttccgggc
    13501 cttcgaagca tcgcctataa gcacgttcac ctcggcgggg cggaagagct caggatcagg
    13561 tttcacgtag tcgcggtagt caagcccggc ctgcgcaaag gccagttcgg cgaactcgcg
    13621 gaccgaatgt gtttcgccgg tggcgacgac ataatcgtcc ggctcgggtt gctggagcat
    13681 gagccacatc gcctcgacgt actcgcgggc atgaccccag tcccgtttgg cctcaaggtt
    13741 accgaggcgc agttcgttcg ccttgcctgc gcgaatgcgg gcgacgccgg aggtgatctt
    13801 cctagttacg aattcaaaac cccggcgcgg cgactcatgg ttgaacagga tgccgttgga
    13861 gcaatgcatc gagtaggctt cgcgatagtt gcgggtcagt tcgaagccgg cgaccttact
    13921 gatgccgtac gtagaacgcg ggtggaagcg ggtgcgctcg ttctgcggga cctcctcggc
    13981 gctgccgaac atctcgctcg atccggcgaa gtagaagcgg cagtctcgga tgaggttttt
    14041 gagcgcggcg agcaggaagt gggttccgtt gatgttggcg ttgagagtgg agaactcgtc
    14101 gtcgaaggaa tagctgacaa agctctgcgc cgcgaggtga tagcattcct cgggcctcgc
    14161 tttctcaatc acctgatgga tgctggcgta gctctcaagt gagccggcat gcaggtgaag
    14221 ctgccgggcg atgtgctgaa tgcgcgacag gcgatgctcg gggtcctcga gcgccactcg
    14281 ccgaacgacg ccgtgcactt catatccctt ggcaagcaga agttcggcta gataagagcc
    14341 atcctggccg gtgattccgg taatcagcgc tcgtttggcc gacatcaagg cgatggtagc
    14401 acgagctgtt tcagccgttc ggcgagccac ttcgaaatga ggtcgagtgc cgtgggcgcg
    14461 atggtctcct cgatctggcc gtactccatt ggcagtccgg tgccggcggt ctggaatagg
    14521 tgattgaggc cagggagttt tatggtagcg aagtctttgt tgccaccgga ctggagggca
    14581 tcgcggatcg ctttcaggtt ttcatccgcg gggacctgca cgtctttttc gccgaacatc
    14641 gccaggaccg ggctgcgcac cgacttcatc gccggcacgg gatcgtagtc gatgaagtgg
    14701 cggaaccagg gggacgacag cattttgatc tgtccttcaa tcatgtcctt ggggatctgc
    14761 tggttgccgg agagctcggg gaggaggctc cggagcttcc tctcgatcac agcggcgtcc
    14821 ttttcctggc gggcgatcgc gtaggcgcgc ttgttgaagg ccagcgtctt ctcgacctga
    14881 tccaaaggaa gacccagtgc gttcatgatc gcggccgtct gcgagacgag cagatcctca
    14941 ccacgaacgc cggaaggggc catcatcacc acgaaggccg ccggcgcgcg ggacgcaacg
    15001 atggcggcga cgttggcgcc ctcactgtgg ccgatgagtc cgattcgttt cggatcgatc
    15061 tccgctcggg tgcgcagcca ggcgaggccg gcctcagcgt cggcggcgaa atccaatgtg
    15121 gtcgccacgg cgaaattccc ggtggacttt cccgtgccgc ggtcgtcaaa ccgaaggaca
    15181 gcgaggccct cccgggtgag gcggtcggcc agaacgagga aaggccggtg gcccatgata
    15241 ctctcgtcgc ggtcctgcgg accagagccg ctgatgagga gagccgcggg aaaggggcct
    15301 ttgccgcggg gcagggtcag cgtaccggcg agcgtaatgc cggcagcctg gttggggaat
    15361 gtagcttcct gttcgtcgta cggaaaggga cgggtgggtt cctgagggcg cggcaatgtc
    15421 cggggcttgc cggcgcgctc gaaatcgagg ggcgccgaga atcccccctg ggaccaagtg
    15481 ccgttaatcc cattaccctt ttcgtccagc tttccgtcgt aggagccacc gacactcggc
    15541 acattgaaat gaagcgcgcc gagcttcagg gagacctctg tggcgcgaag gcccatggca
    15601 ccttggtcga ggctgtcgag ggtcacggtg agggctccct tagcgtcttt cttgatgttc
    15661 aactgaagtc gcagtgtcgc tccgccggca ctgagggatc cctgccacgt cccttcaatt
    15721 ccagtttgag cggagatcgt gacggccgaa acggccagcc acgctccgac gagagtaggt
    15781 ctcactctac gagtatagag cggcagctac ctcgactcgc gggcaggcta ctcctggaag
    15841 ttgaatgtgc cgacgaaggc gctccgcttg tccgggctgc tccaattcgc gttcaggccc
    15901 ttgccacgga aggtgtgcgc agaagcgacc gcaggcattg cgtggtcgag cttggtcaga
    15961 ggggagtagt agacatcctc gttatgacag atgtgatcgc cctcgccgat ggggcgggtc
    16021 tggatcctgg ccaactcacc cgcggtcacg agcgccttca tgctgtggac gttgtcgtcg
    16081 tcgaacttga agtcgatggg gcggtactcg acgcgaacga tgtcctggag cagatcgccg
    16141 gccagacgtt gggcgaaact cttcagggcc tggcgctgct cgagaccagc gcgttcatcg
    16201 acgatgagga tcgacttgac cggatacgcc gtgcgggtcg gatcgccaag cgtgtggctg
    16261 gctcgaatgg cggcgacgac ggacaggccg tcgagcgtga cgccctgcca gttgcctttc
    16321 tgcaccttcc agccgaaaac ggccatgtcc ccgccgaaac cgacttcgcc gttggcgaag
    16381 caggggcctg tgtagacatc cgcggtgcgc gcttcgacgt actcgccgta agcttgattc
    16441 ttcgggagag cggcgccgga aagcgacgca gcaagaaagg acaccaacat aatcccagac
    16501 agatacttca tcccagggcc tcctcagtga gcttgtacca gtgtagcgcg ggccggagtt
    16561 tcccgacaac cccggcccgt tttgtgagga atcagaaggt cagagcgctt tgtagcagtc
    16621 gcggcagggg ttgcactcgt agttgtccac gagcttaccg tccggtccga ggttgagctg
    16681 ggtcttcaca caccagaaga tgcgctcgtc gccccgctgg agggcggaat cggcctctgg
    16741 gtcgacgtag agccccttcc agcggatgtt ggcgcagcgc tcctcctgca tgacgttgaa
    16801 agcggagcct gccatggcta tcccctcctc cgcgccgccg cgagcaacgc tcgcttcacg
    16861 gcggtgcgcg caatctggac tttgtagcca ttgccgctca gcggttgcgc accggaaacg
    16921 gcagcctgcg ccgcctcctc ggccacgccc tcatcgatgg tcttaccgac gagcaactgg
    16981 tccgcttgca aggccgccca cggcgtgggc gccacatggc ccatgacgac ccgcgcgcta
    17041 gccactgccg cccccttcat cttgagagcg acggacgcgg tggccagagg ccaatcgagc
    17101 gcctcctttt gccgcacctc gtaggtggcg ttgcgaacac caacagccga agggacgatg
    17161 atctcggtca ggatctcgct cggttgaagc gccgtttcgc gcgcggtatc gattcgcggc
    17221 attacaaaaa acttctcgac ctccagttcg cgcgtaccgg acgccgatgc gagcttgacc
    17281 ttggcgccga gggccaccag cgccgggccg aagctcgacg cgctgacgaa gtaggccgga
    17341 cctccgctga agatagcgtg gtagcggttg tctccctctc tgacgaggct accgcccttc
    17401 gccaggagac ccatgcccat gcggaagtac caacagcgcg gccgctggca aaggtcgccg
    17461 cccacggtcc ccatgttgcg gatctgcggg ctggtgactc cctgtgcggc ggtgatgagc
    17521 gacggatact ccgcgcggac ggccgagctc gccagcagtt gatcgagagt gaccgtcgcg
    17581 ccgatgcgga gaccctgagc ggttttcgtg atgccgccga gttccttgat gcctttgatg
    17641 ttcacgaccc gtttcggggt gtggacgtac tccttcatca ggctgagctg gtccgtgccg
    17701 cccgcgagca cgtcggcctc gccccactgg ttacccagga gagccagggc ctctttcaga
    17761 gtggccgggc ttgcgtattc gaaggattgc atcaggctgt cctccttgcc agggcctcaa
    17821 ggaccctgtc cggcgtcagc gggatgcgtg gaacccgcac gccgatggca ttcgcaacgg
    17881 cgttggcaat cgcgcccatg cccgggacga ccggtggttc acccaggcca atcacgccgc
    17941 gcttgtcatg ctcctcgttg atgtcgaggt gcacgacgat ttcgccgatg tccgcgatac
    18001 cggcgagctt atagaactcc atgtcggcgt tcaggaatcg gccggtcttt tcgcacatca
    18061 ggcgctcctc gtacagagcg ccgcagatgg acatgatgca ggcgccatag acctggcttt
    18121 cggccgtctt tgggttgatg atcaggccgc agtcttgcac cgccacaagg cggttcagct
    18181 tgacgacgcc ggtctcgata tcgactgaca cgtccgcgat ctgggcaccc ccgacaccct
    18241 ggctgatcag gcccatgggc tgcttcgggt tgttcgtgcc catggcttct atcggcttga
    18301 cgccgatctt ctggcaggct gccttccagg tcatgctctt cgcgggatta cctttgacct
    18361 ggatcttgcc gtccacggct tcaagttggt cggccggtgc gccgagcgcc ggggccaccg
    18421 cctcgtaaac cttggcgagg gcgttcagcg tcgccttgcg tgttgacacc gacacgccgc
    18481 cgacggttgt ggaaccgccc gaggcgccgg actgcgggta gctgctgtcg ccgatcttca
    18541 cgttgatctg cgggactgtg agacccagcg attcagccgc caccatggcg atgacagtgc
    18601 gcgtgcctgt cccaagatcc tggcttccga gttcgacatc caccgaaccg tccggatgga
    18661 tccgcgtgcg gcagtcgctc gggtggccga ggccacccca agtgccgacg ccgatgccga
    18721 ggcctcgtct catcggacca gaacccgcct ggtcgcgtgg atgccaaagc ttcttccact
    18781 cggccaattc ggcggccttg tcgagctgcg cccggtacac ctcggcgcga gcggtataat
    18841 ccgcgttctt cttgaacacc tccagcgggt ccatcttgac ggcggctgcc atgtcatcta
    18901 tggcgcaaca ggtgacaaac gcagcctgcg gatgattggg agcgcgccac gcccgtgcac
    18961 cgccgttatt cgtgacgaca ccggtgtggt tgactcgctt gttgggaatc tggacgtaga
    19021 cgtaagggag ctgcgtcggc gaaacgccgc cgccgctgac gccgctcgta ccccaggatt
    19081 ccgattgcca ggcggtgatg gtgccgtctt tcttcgccgc gagtcttact ttggcgaaga
    19141 tggacggccg gttgccagcc atctcaaggt cggcgcggcg gtcgagaaac aacttgaccg
    19201 cccggccgcc ggccttcttc gaaagtttgg cgcagatcat tccccagcgg tctgcggcga
    19261 atttgctgcc aaagccgccg cccatgtggt cctggtgggc atggatgttc gctgcgggaa
    19321 cctcgagggc gcgcgccagg tcaccgccga ttccggacaa gttctgggtc gaaggccaga
    19381 attcaatctt gtcgcctttc caggcgatga cctggccgtg cgactcttgg gtgcagtgcg
    19441 taagcaccgg gcaaccgtaa tacccttcgg agaccgtgtc ggcatccttg aaggcttggt
    19501 cagggtcgcc cgtgaggatc tcgccagccg gcttgctccg gctaccggtc ttgccgaggt
    19561 cctcttcgtt gacgaggtga ggaaggactt cgtagtccgc tttgatcgca cggacggcat
    19621 cggcggcaat tgtttcgctg gtagccgcga cagccgcgac ctcggtgccc gcccattgga
    19681 tttcggtgcc ggcgggcgac accacttcaa ccgccatcac gcccgtcatt ttctcggccg
    19741 caccggtatc gatgctccga acgcgggcat gcgcgtatgg cgacgtcagc aatgctccga
    19801 acagcaggtc aggcgtatgg atgtcatacc catacttcgc tttgccgctg gctttcgcgg
    19861 ggccatcgag gcggctcatc cgcttccgca tcacgcggct ctttcccaat ggaggccaac
    19921 tgtagttcgg gtcaggcact ggctgctcct ttcatctgtt tggccgcttc caggactgcc
    19981 ttgcggacgc ccatgtaggt accgcagcgg cagaggttac cgccgagtcc agccttgacc
    20041 tggtccatgg ttgggtcggg gttgtgatcg aggaagccct tggccgccat cacgaaaccc
    20101 ggagtgcaga agccgcactg ctgggcgtcg ttgttcacga aagccgcttc caccggatga
    20161 agccggccgg ccgtggccag accttcgatc gtttgaatcg gtttgccctg ggcgtcaatg
    20221 gcgagcacgg agcaagcgta aacaaccttg cccgccatga tcaccgtgca ggagccacag
    20281 gtgccgcggt cgcagacttt cttggcacca gtcatgtcca ggtgatcgcg aagcgcgtcc
    20341 agcagggtga ctcggggttc cacgctaagt ttgacaggtt tgccgttgat ggtcagggtg
    20401 atgggaacgg ggcccggtcc agcaacgtcc gctgcagcga ccggctccgc ctgagcttct
    20461 gtttgtaaga ttccgacagc cccgctaccg agtcccacgc cgcggatgaa accgcggcgg
    20521 gagaacccgg ttttgggttt cttctccacg tcagggtctt tcctcagact cactagacac
    20581 cttcctttcg tgtccaaaaa aacttgtgct ccgactccgg tattctgaac agcccgaaat
    20641 gactatagca gaagggaaca aaacggaaca agcctgaaga attgcgatca ggaggcggcg
    20701 gcttttcgcc gcgtggcggt ttcttggcgg cgctcgaccg gtgggaggct gaggctcaag
    20761 gaggcggttg tgcggggttt tggggtaaag tctttcggtt gtgaacctat agaacgcacg
    20821 gatgggactc aggcgggggg tactgcggat caacctgcct tccttgagcc gttcttacgt
    20881 tcgtcacgac ttcgatccga gcaatctaca aaccatccgg gaagtgcctt tgttttcagc
    20941 tagaccgagg ttcacgaagg ggagggagcg gcaaaaactg cacgcctttc ggaacagctc
    21001 gtgccatttc ggcacacaat ggagagctag atgataacga acgatagtgg atatctgcgc
    21061 gaccttctcg accaaccccg cgcgcttcga gacaccattg ccgccctgaa cgtcaagccc
    21121 gcattggcgg cgcttgcggc gcgccggttc gaacggatcg ttctcaccgg catgggctcg
    21181 tcgctgcacg cgcttcaccc gctgaaccaa cgtctggcgg ccggaggctt ctgcccggtg
    21241 atggtggaga cgtcggagtt gcttcacggc ttgcgagggc tgctgaccgg agagacgttg
    21301 ctggtagcgg tttcgcagtc tggacgaagc gcggaaacgg tgaggttgct ggaacaagag
    21361 ggtccgcggc cacacaccat tggcgtgacg aatacgccgg attcgccgct ggcgctcgtg
    21421 ccggacgccg cagtcgtgac ccgtgcgggc gccgaaaact cagtggcatg caagacatac
    21481 ttgtcgatcc tggccgcgct cgactggctg ggaagcgtgt tttgcggcga tgacctggag
    21541 gcgatgcttc ccgaacttgc tcaggcgtcg atgttggtgg acaactacct gacgcgatgg
    21601 caggaccacg tggaggaact gcactccgag ttggccggcg tcgagcacgt gttcctggca
    21661 gggcggggat gttcgctcgc gacagtgggg aacggcggac taatcctgaa ggagtcggcg
    21721 cacgttcacg ccgaggggat gagtgcgccg gcgttccggc acggcccgat cgacatgctg
    21781 cgcgacgacg tgttcgtgct cgtgttcacc ggcgaaccag ccaccgcacc actgaacgtg
    21841 cggcttttcg acgatattcg aaatgccggc ggccggacag cgctgatcag cgagaccact
    21901 gccggagcgt ttcggttgcc ggtgggtccg gcacgcctgc ggccgatcct ggagatgctg
    21961 ccggtgcaga tgatgtcgct cgctctcgct cagctcaaag accacgagcc cggcgtcttc
    22021 cgacacacgt cgaaagtgac gacggtggaa tagagcggcg ggggcccaag gcccgtcagt
    22081 ttttcacttc cgggatcgca ctgggggcgc gggagacgcg gccggccatc gcctcatcga
    22141 tcgcgatgtt atagacgccg agggtctgcc cggtggaccg gtccagttcg ttgcgtgcct
    22201 tgctgtacgt gctcagtgcc gtcacttcag ccgatcgagc ctgggcgagg tcgcgctgcg
    22261 cctgaattac aaagaagatt gtagaggcgc ccagcgcgta tttcttttct tcggcgtcga
    22321 gcgtctgttc ctggagcacc cgtgccttca tggccgcctg gtagcccgct cgcgcctgct
    22381 gcacggcgat cagagagttg gtcacgtcaa cgcgcacctg gtttacgagc tgctgttgcc
    22441 ggatttcctg ctggcgcagc gagagctggt cgcggatcat gtccgcctgg gccgcccggt
    22501 tgcggagcgg gatgttgagc tggataccga ttccgtagtc agggaagttg cgacggaaga
    22561 tttgcgacag caccttgccg taaccgccga tgaagtaagg gtcgacgctg ttcgggtcgc
    22621 gtggcgtcac cgtgccagcg ggctggcccg ggaacggcgg aaccggcaat gagtttatgg
    22681 agccggacaa gccgttgttc tgtgcgtatc cgaacaagtc gagcgaaggc cgcaacgcgc
    22741 tcttggtgcc ctgcaagccg atcttggtgt tctccaccag aaggcgggtc tgcgcgatct
    22801 cgggacggct gtcgagcgcc cgattcacca ggtcctgaat cggttcgatg gcctcggaat
    22861 ccggcatgcg catctggtcc gtcgggacga tgcgggcgtc ggcaaccacc gggctggcca
    22921 ccccggtccg gctcaaagca tctttgatga tcgtttcctg ctggaggacg cgggtctcgg
    22981 aaacggtcag ctcctgctgg ctgcgggcga cctcggcttc cgctcggacg atctcgatcg
    23041 gagccagagt gccgatctcg acctgcttct tgttgtcgtt gtacagcttc tcagccaacg
    23101 cgagcgcctg ctgctttact ttcacgtctt cgttgaagct gacgaggtcc cagtacagcg
    23161 cgacgaccgc cgacacagtg gtgatgacct gctgcttgaa gacgaggtcc gaaactctcc
    23221 ggtcgttcct ggcgatgcgg atgttccggt tgttgacggc ggtgccgaac ccttgaagga
    23281 gtctctgggt gatggtgaga tttaggctgc cgctgatgta ggggttgaag tcgctgcgtc
    23341 cggagttggt ctccgccggg ctgttgatcc agtcgaactc gagattcgtc ccggtcagga
    23401 agccttggcg gaaactcatg ttgcctgact gattcttgat cacgagcgag tttgtgccgg
    23461 tggtgaagga gtttgtttgc gggacagtgc ggtgggtcca gctatactgc gtggaaatca
    23521 ccggatcgag attcggcagc gccgtgccgg tggtggtgat gattgcggcg ccggtcgacg
    23581 tagtacctcc gcctccaccg gtgcccccgc ctccaccgcc gccccccgct gttccggtcg
    23641 cctgggcgag agcgctcgag ggtccggatg tgatgctggt cgggataccg cggagcaagc
    23701 cgccagcctg ggcccggaga atgtcggcgt ccgcggctcg cgggccatag cgcgaaagcg
    23761 cgatgtccag gttgttttcg agcgcaaggg cgatcgcatc ctgcaatgag agataaatcc
    23821 ttccggcacg gagcaggttg tcgatccggc tggagttggc gaggttaatc ggcgcctctt
    23881 cccggtattt gtaggctctg gtgaagcccc cgaagaaccc acccgatgcc tgggatgagt
    23941 tgttgagcga agctccggtc tggccggaga gcggcgccac catcaagaga acacacaata
    24001 ttgccgtaac gcgtttcatg cggttttttg acctcggtcg tgctttggtt cggcctcaaa
    24061 gttgaatcga cgcaaacaga agtgcgcttg ttccttgaat ttccaatgct atagtagcgt
    24121 tctgtgaacc tgcaagctga agaagttcta aaggttttca acgagactgg cgcctacctg
    24181 cgtggccact tccggctgac cagcggcctt cacagccccg agtacctcca gtgcgccctc
    24241 gtccttcagc acccccgcca tgcggaacgt ctcggccagg cacttgcaga ccgtcttggg
    24301 gagattctag ccggtgcacg ggtcgacgtg gttgcttccc cggccatcgg cggtctgatc
    24361 atcggccatg aggccgcccg cgccctggga gcacgattca tctttgcgga gcgggacgcc
    24421 gagaagaaga tggttcttcg gcgcggtttc acggtccagc cgggcgagac cgcggtggtc
    24481 gttgaggatg tcgtgactac cggcggcagc acgcgcgaag tggtggaaac cctccaggca
    24541 gcgggcgctc gggtgctggc agccgggtcg atcatcgacc gtagcggcgg acaggccgac
    24601 gtcagcgtcc cgcgcgtggc gctggctact ctccaagtcg tgacctggca gcccgaggct
    24661 tgcccgcttt gccgggatgg ctcgcccgtc accaagccgg gctcgcgccc ggtttgactt
    24721 cgccatgcgg cgcatcaggg tcaccgtcgc ttacgacgga accggctacc acgggtggca
    24781 ggttcagccg gggttgccga cgatccaggg cgtcctggag cgagtggcgg aggagatcga
    24841 gggcaagccg atcaaagttc acggctccgg ccggacagac gccggtgttc acgcgcttgg
    24901 ccaggtggcg gcgttcgact tcgagaaccc gattcccacc cccaacctga gaagggcgat
    24961 gaaccggctg ctgccgccgg agattcgcgt gctggatgcc gaggaagcgc cggcggactt
    25021 tcacccgaga caccgggcag tggccaagac ctacgaatac cggatcttcc gcggcgagat
    25081 ctgcccgccg ttcgaccgcc gttacgcgca tcactaccct tacccgatgg cggtcgacct
    25141 gttcgtcgaa gcggcgccgc tgtttgaggg cgaacacgat ttctccgcgt tcgccgccgc
    25201 cgacgagaag gacgcgctcg gcgcctcgaa ggtgcggacg atcttttcgt ccagcgcgga
    25261 gatcgttggt gagtggatgt tctaccgcgt ccgcggcagc gggtttctga agcacatggt
    25321 gcggaacatc gtgggggtac tgctcgaggc agggaagggt aacctcggcg aagacgagat
    25381 ccgtgggtac tttgtgcccg gctatccggg caaagccggc ccgactgtgc cggcctgcgg
    25441 gctttttcta gtgtcggtgg agtattgagc ggaggtcagg ggaacaggca gctctgcccg
    25501 ctcgcaaaca gcgcttagag cagagtaggc ctgtccccct cgggtgacgt tgaagccgaa
    25561 agtaacgccc acagcgtcgc cgcgcagatg agcggcagga tgccgaagcc gatgaagacc
    25621 ggtgtgtagg aaaagtgatc caccgcccag ccggtcagca gtgtgaacag cattcctccg
    25681 aagcccgatc ccatgctggc aagtccatac accgaagcca ctgcattctt cgggaagaca
    25741 tcggcgggca tcgcaagcat actggcggac gcgccggtgt aaccgagcat cgcgagcgaa
    25801 acgagaccca tcgccacgcg catattcgtc accaggacgg cggggatggc ggaggtcacc
    25861 agcagcgcga agatggtcac cgcagtgcgg cgggcgttca tcacggggag tccgaggccg
    25921 atcagccagc cggaaagcca gccgccgatg agattgccga gcccggcgac tgcgaacgga
    25981 atccaggcat agcgcccgat cgacgccatg tcgaagccgc gggccgtctt caggtattcc
    26041 ggaaaccaga agatgtagaa ataccagacc gggtcgatga agaccttcga aagcgtgaag
    26101 ctccacacaa aacgcgttcg gaacaggtct aacaggctga agcttgttcc actcgcagcg
    26161 gcacggcttt ccggctgtgt caagccgggg gaccggtaaa cgggccacca cgcggcgagc
    26221 catagaaatc ccaaaaccgc cacggcgagg aacgatgtcc gccagccgaa ctggaggatc
    26281 agccaggcta caaggggcgg ggcaaggatc gctcccaccg ccgagccgct gttgaaaatg
    26341 cccgaggcca gcgcccgctc gcgttccggg aaccactcgg ccaccacctt caccgccgcg
    26401 ggccagtttc ctgcctcccc catcccgagg aggaaccgga aggcgccgag actccaggcg
    26461 ccggtggcca gggtatgcag cgccgcagcg gcggaccacc aagccatgca gatcgcatac
    26521 cctacacgcg tgccgaggcg gtcgagcaac gctcccgaga caccgttgag gatcgtatag
    26581 gcaagcagga aggcgaacac gactcgcgaa taatccccgg cactcatcgc gaactcctcg
    26641 cggaggacgg gggcggcgac cgacagtgtc tggcggtcga ggtaattgat tactgtcgcc
    26701 agaaaggcga acgagatcat caaccagcgc aggcgcggca tgatcgggcg gacattacga
    26761 ggctgcgccg tggatggctt cgttcacgag ttggcagttt tcgcggacct ggtctttgtg
    26821 cttggggaac atgccgatca cgacggcgtc gtccggcttg gcgtactgga aggcgagccg
    26881 cagggcggag agcatgcgct ctcgagtgtc gcaatgccgg gtggcgccgt agaccttgaa
    26941 gatgaggcac tgcctggagg tctgccggac gcgcttcagc atttcctcac ggtcggggtc
    27001 ccagaaaagc tcgtcgtcca cgggacggcc ggcgaggcgg ctgacctcct ccttcgtgcg
    27061 gctgatgtta tagacgcagg tcatgtagaa atcgacgtcc cagcccttgg attcgacgta
    27121 gtcgatcacc tgcgggatgt gggtgcccag gccgacgcgg gttccagtgt cgcggatgcg
    27181 cttgaggttg tcgcgcgcgt cgtcgatttt gccggcggcc cagaggcggt ccgtcagcga
    27241 cccatggtgg tagatgccga tgggagcctc gcccgccatc tcgcggaggt tacggtcgaa
    27301 gacgatctcg gtggcggtct ggccgatcca ttggatcgtg cctccctcct ggcggtactc
    27361 gtggagcatt cggatgatgt gcttgtcgcc gcgggactgc cacgagttga tgccggcgtt
    27421 ctcgcagtcg cggagcatgc gcttgatgtt ggcggaggtg aagtagtcga ccatctcgcg
    27481 gctcaactgg ttgttgaggt gggaggttcc gctgacaggg ttgccgccga cgatgagccg
    27541 ggagacgcgg tgcttgccga agggaaccgt cggcactccg ggcgacttcg ccgccgcggc
    27601 ggccgccgtg ccgccgagcg cagtcagcaa cgcctcacgc ctgctcagat ccatgactca
    27661 cctccggcta tggcgatcat agcagaagac ggtccgaatc tccgggaggg agtggaaacg
    27721 gcaacagaaa gaccgccttg gaacttgttg gctccaaggc ggtctgagct gctggaacag
    27781 aagacgctcc tacggagcgc aacttgtctt cttgaggttg gtgtttgccc aggcgctgac
    27841 ctggcagcct gcgttcacac tgctcgtaac gccactcttg aacgactcgt taaggttctg
    27901 aagcagttgg tcaagcgtgg cataagtgta acctgacggg ggagatgcaa gagccccgcc
    27961 catcacttga gcggcggtcg tcagaatctc ggagatcttc ttgcccttga gatcgtcact
    28021 ccccgagggg cagtagtaca ggtccccgaa cggcaggctg gccgggtggg tgagaagatt
    28081 gtttgcgctg gcgtccacgt tcaacttcag agccaccacc tcgccgccca gtttgcctgc
    28141 cgatgtcgtc tgactacctg agttggtaat cgtgtagttg ctcgttagtt tcccggatgt
    28201 gccggtctgc ggcaagaagt tctgaatctt gcccgcgccg tccgccgcca gagagtaggt
    28261 gctgccaact tgaaagtagt tcgacgccgg atagagcgtg gagaagttgt cggtcaatag
    28321 tttccccgca gtt
//
LOCUS       DATIFQ010000188        19773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2169975, whole genome shotgun sequence.
ACCESSION   DATIFQ010000188 DATIFQ010000000
VERSION     DATIFQ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19773
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2169975"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..200)
                     /locus_tag="VLE22_08615"
     CDS             complement(60..200)
                     /locus_tag="VLE22_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14509.1"
                     /translation="MLALLRAAGAAWESLYEKDVEHYSEMPEFTRAMAEDNFRRKLNG
                     HL"
     gene            complement(1203..3011)
                     /locus_tag="VLE22_08620"
     CDS             complement(1203..3011)
                     /locus_tag="VLE22_08620"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HSB14510.1"
                     /translation="MESDREFSRRSFVRAAGVVSAALAQPTPAFVSSSLSHRLEGKTQ
                     LSVTSDGVIHAETPTLLAKLEKGFITSLKSKASSEECITGFDTGKFDALQLVYGRGET
                     VDVGEQKFGKVETRKISDHRAEVIFQNWDGDGVITISVDPASGDLVVEPSAFSSRPGV
                     RACRWRLKGLRRDLKLVAPFFQGVSLNLDDSLIRSSHWDWPHRWEAGLAILQSSSGGF
                     SFHTQDDRYRYKALHVGSDDDPYMIGLDTEAYGPIDANLAAGGLAWRINVHQGDWKVP
                     AERYRSWLWEAFSLAREEQRREPWLHALAMAISWCPGDIAILENLAKQVDPKKVLIHF
                     SSWRTDGYDENYPTYVASESAVAFLARAHEMGFHVMPHFNVVETDPNHPVYAQVRDFQ
                     YRDIETKKLYGWSWVNNRAIGVPESNASRLNHRREKVMVKIHPGLTTWRSILSSRIQE
                     AARQYALRDVFVDVALNTFNLHNCLVENMTSTEGVNRLIHEIAGLGLSVGAEGLNETF
                     FQGLSFAQVHLFRNPRGATDWLERTGGCPLNSFLFGKLCRSFGYSGLSGRNADEELRA
                     QVHEANGALPTITIRSAAEITNPTPAVKRALDRARR"
     gene            complement(3038..3319)
                     /locus_tag="VLE22_08625"
     CDS             complement(3038..3319)
                     /locus_tag="VLE22_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14511.1"
                     /translation="MAAGGFWKLSKLVQNPVRAGQYRAIAIRTVDSLTNRYMATGDAV
                     SEGRMLVHATLHKPAGLGVDESMILGDYYYLKLLLEILNEGRAVGRGPL"
     gene            complement(3413..4276)
                     /locus_tag="VLE22_08630"
     CDS             complement(3413..4276)
                     /locus_tag="VLE22_08630"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HSB14512.1"
                     /translation="MMAVFHVSILSRPFSLCESTGTLAVAAIATLLAVPQASGADYRE
                     ALSTVETRLGRLCAIAGSEYPDHTVNGKWKLREPSNWTAGYFAGMLWLMYGQTKDPVW
                     LERAQRWTNPIGEFRHDKRDLNFGLSFMPTFVAGYRLTGDTAYRRIALDAARSLAARY
                     MPDGKYIRSWGTIGNTKQQEFVIIDCLIDLDLLYWAARETGDGKYSEIADNHAGVTVD
                     AAIRPDGSSIQVVQLDTRTGKKVAGRHKQGLSPQSCWSRGQAFGIYALADAYRFTGKR
                     RWLEAAERLAA"
     gene            4457..4864
                     /locus_tag="VLE22_08635"
     CDS             4457..4864
                     /locus_tag="VLE22_08635"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB14513.1"
                     /translation="MDYLLEMVQKPFAELRGEQQIAAVLIVLLCVFIAFSAVFQAVRG
                     MKGRRRYWRHRQTGPIDVSWRSSLGSPRREQGRCVDMSAGGLRMELQDPILVGTPVSF
                     HLLQTKRAGTAFVRHCTPDGSKFYIGVEFAGSR"
     gene            5309..7711
                     /locus_tag="VLE22_08640"
     CDS             5309..7711
                     /locus_tag="VLE22_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HSB14514.1"
                     /translation="MKKALLFAGAAVITLLAVAAVYVSRLNVDRFRPQIQAELQRQLG
                     RQVALGAMGVRIFPLSIRINDVTVAEDPSFRTDRPFASAREVSVAVSLFPLLRKEVHV
                     QSFVLRDPVIELVQNRSGKWNFSSLGAGGGGEGGGLSVAELRIENGRISVSELADRKP
                     ASVYDHIGIRLADFGPGKVFRLNASAQLPGAGKDVLILEARGDPAHAQGALLAGQVQI
                     QQAPISALRSFLHTGATKADGSLSTAMDFELQNSALKANGWVELANLRTGGADLGRPI
                     RLDCKISHNLDTGVLKASEVTARIGNAPVVISGELDTRASRIDATIRTAGAGLKEVLT
                     AASAFGISGISGTGQLTLDARIRGPLDRIAELECTGRASIRDATLSVPALTKPLQVRS
                     ADLDFAGRSAGFENLSCSLGSSTLRGNVMVRNPASPEAEFNFDIDRLDLAELRQLTAT
                     NQPSRSEGGRSSGSFTAKGSVHVGTIAYNQMVLKDVRSECVLSRGILTLSPLTAELFG
                     GRQSGSITVNLQSQPPTVALKTNLDGVDANQLLSTTTSLKNMLYGLLAAKGDAAMQLS
                     SGGDAARTLNGNLNIQLSKGKLGGINLMNQMASLGKFVGFARNSEPFTDIVQLSGDIQ
                     VRNGVADTNNLLIQTEGASVSSAGTFSLVDQSLNLRLTAVLDRDASQKAGGSRVGGFL
                     TTALVNGKGELVIPAIVTGTFANPRFAPDAARIAEMKLKNLMPSTANPGSMTSGIVGI
                     LTGKSGGERPAQGTTTSQQENVEPAQAPGAKPPEAPQRKGVLGILDSLRKKASEPAKK
                     NP"
     gene            complement(7728..8279)
                     /locus_tag="VLE22_08645"
     CDS             complement(7728..8279)
                     /locus_tag="VLE22_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB14515.1"
                     /translation="MRSRVLWMFAVTSAALLGADPATSSIAKIFDQEISVIEREIVPL
                     AEEMPAAKYGFVPGTGAFRNARTFHQQVTHTAALLYMYGASILGEANPSEPGPNDNGP
                     ASLATKEDAVKYLKDAFGYVRKAVSSVTSENLTGMVKSAYGDFQVPRASMVSAAIGHT
                     WDHYGQMAVYARLNSVIPPASRR"
     gene            8579..9181
                     /gene="msrA"
                     /locus_tag="VLE22_08650"
     CDS             8579..9181
                     /gene="msrA"
                     /locus_tag="VLE22_08650"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSB14516.1"
                     /translation="MTPADKGSFPEPAVDVKPATGKQTAVLAGGCFWCMEAVFELVEG
                     VDDVISGYSGGTKETAKYDIVSMGRTGHAEAILITYDPSKTSYGQLLKIYFSVAHDPT
                     TLNRQGPDTGPQYRSAIFYANEEQKRVAEAYIKQLNDAHVFRNPIVTQLVPLDKFYTA
                     EAYHQNYAVQNRSNPYIVNVSDPKVQKLKKMYPGCVRKRK"
     gene            9184..9696
                     /gene="msrB"
                     /locus_tag="VLE22_08655"
     CDS             9184..9696
                     /gene="msrB"
                     /locus_tag="VLE22_08655"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSB14517.1"
                     /translation="MNRRTFLMGLSSSAFLLGKGKTVKIVEFSDTGERKGLVEVEKIV
                     KTDEEWRRQLSPAEFEVTRKGGTERAFTGKLNKNYAEGIYRCVCCGTALFHSKTKFDS
                     GTGWPSFFAPIAKENVVEKLDATLGVTRIENQCARCDAHLGHVFDDGPRPTGRRYCMN
                     SVALTFVKAH"
     gene            complement(9739..10650)
                     /locus_tag="VLE22_08660"
     CDS             complement(9739..10650)
                     /locus_tag="VLE22_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB14518.1"
                     /translation="MTVINRRQFAAAASGALAGLAHAQTSGGRFIKSICRVNFPKQMP
                     IEECFRQAKNAGFDAIEVPMLDEFVPTATADQVKAIGDQAKKAGVAIAALWVSPLGEN
                     PLNHPDAEVRAKGVEAIRKATEFAVYLNCDGLLLVPGYVGYGNKFFQGYEVTYERITA
                     ELRKALPYAEQAKVVMAVENVGNRFLVSPLDARSFVDQFRSPWLSFFFDIGNILTHGF
                     PQDWILTLGPRIKRLHAKNRRATLRGPQGPPGGLLEGDVDWKAVMSALVQVNYRGFIS
                     PEYGYNPDDPDQLKKLSAELDTILAMA"
     gene            complement(10667..11773)
                     /locus_tag="VLE22_08665"
     CDS             complement(10667..11773)
                     /locus_tag="VLE22_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14519.1"
                     /translation="MNRRHFLIGSMAAAAPSVSAADSGAIGTGFIGTGNRGSHLLELT
                     LDEPTAKVVAVCDIKPDRLDRAATIAARDQPTTFSDWRKLIDRKDIGAVFIATPCDLH
                     VEMAIAALKAGKHVYCEKPIGITAESIRELVKVARSSKTVFQTGLQRRSEPRLRGTIS
                     RIHEGVAGKVIYVKAQRHNDSDLAHDGSSADWFFFPNRSGDLITENSVHNLDVCNWAI
                     NSYPERATGFGGNIMYVNDPPGRTNMDGYTLSYEYANGVKLAFTQVAFHPAKMPYAWQ
                     HTFIYGTKGAVDLDTAMFYPHEGGEPQVLAPPEERAPRDRHIADFYECIRTGKKPAAD
                     IIVGASGALTSILGREAINRKKILTWKELGVDFL"
     gene            complement(11842..13173)
                     /locus_tag="VLE22_08670"
     CDS             complement(11842..13173)
                     /locus_tag="VLE22_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14520.1"
                     /translation="MNRRQFLHTAAAGSFARAVAAEKPKRVGLIGPGWYGKCDLIRLI
                     QVAPVEVVSLCDVDSRMLADAADRVAARQASKRRPRTYPDYRRMLAEKDLDIVLIATP
                     DHWHALPMIAAVQAGADVYVQKPISVDIVEGRAMLAAARKYKRVVQVGTQRRSTPHLA
                     EARDRIINEGKLGKIGLVEIYCYYHMRATGNPPDTNPPAYLDYDMWTGPAPMRPYNEL
                     VHPGRWRAFMEYGNGIVGDMCIHMFDMVRWMLGLGWPKRISSSGGILVQKNTKANISD
                     TQTATFDYGDMSVVWQHRSWGHPPDPKYPWGATFYGEKGTLKASVMSYDFTPMGKDDP
                     AIHRDVTYELEQYPEDKTEERLERHVAPAIRHHMQDFLSAIASRGKPVADIEQGYIST
                     TSCILANLAMQLGRTLAWDPVNHTVLGDSEANRLLRRPYRSPWVHPEPDNV"
     gene            13281..14285
                     /locus_tag="VLE22_08675"
     CDS             13281..14285
                     /locus_tag="VLE22_08675"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSB14521.1"
                     /translation="MPTRLVLMCVLCTFVVSAQASAGWRKGQGYGWVYGKQDEVGALN
                     TVTGPEHVLKALRAVKTGRVFDLGVPLDRRSYKWPGHSPTEVMSFRSPEGLKRGKDLG
                     PFAADPKGLGFHSCALFISDNVGTQIDGLGHITTGADNHWYNGFREGDYGTDFGIRRA
                     DADSIPPVIARGILIDVAGWKGVGALPSNFAIGSRELQSALAAQGTDVDPGDVVLIRT
                     GVLRYWGEAGENHAKVAEHDSAGLTLEGAKWLVEQKGAVMIGADTSGLEVGADPALPG
                     ASIPVHVYLLVEQGVHIGELHYLEGLARNKVYRFTYVAMTNRVKGAVAGFAMRPLAIE
                     "
     gene            complement(14295..14780)
                     /locus_tag="VLE22_08680"
     CDS             complement(14295..14780)
                     /locus_tag="VLE22_08680"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB14522.1"
                     /translation="MSRALLTAVILFAAAATLAAQQKLTPLDEAAYKRVIASHKGKVL
                     LVDFWATWCSPCLEELPLLVKLEAKYRGKGLSVVTVSCDEPEETNKALEVLKQHKAPL
                     GYLKRVDDDEKFINSVDPKWSGALPGLFLYDRNGRLVKSFVGETDMAALEKAIRKLLA
                     G"
     gene            complement(14777..15355)
                     /locus_tag="VLE22_08685"
     CDS             complement(14777..15355)
                     /locus_tag="VLE22_08685"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSB14523.1"
                     /translation="MKRLLWLGLTASFGAVLFGQEFKLGSKVGDFNLSDLKGNKVQFS
                     QLKGDVTVLMFIATQCPISNDYNERMNAVVADYAPKGVKFVAVNANSSEPPAEVEQHA
                     AEHKFSFKVYKDENNVVADRFGAQVTPEIFVMDKNGTIQYHGYIDDSRNSANIKTQGL
                     RMALDSVLAGKPVERAETKAFGCTIKRVKKAS"
     gene            complement(15397..15759)
                     /locus_tag="VLE22_08690"
     CDS             complement(15397..15759)
                     /locus_tag="VLE22_08690"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB14524.1"
                     /translation="MKTKAIFVAVVILLWATASGLALQKGKQREDPNVRSVQGVVVDQ
                     KDSPVEGAVVKLKNTKSLQVRSFITQSDGTYYFHGLSTNIDYELKADRGGASSEAKTL
                     SVFDSRKKAIINLKLESK"
     gene            complement(15808..16236)
                     /locus_tag="VLE22_08695"
     CDS             complement(15808..16236)
                     /locus_tag="VLE22_08695"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB14525.1"
                     /translation="MRTRRKTAGGRRSEAPIYPILAVACILLCLPLPAAAGRPAKDQK
                     RQVAVAMIAGTVFQESGFLLRGAEVAISRDPQAKSSHKFKKVKVVSDSRGEFVVRVPA
                     EPMRYTVSVSAPGFRAQEKSVSIGGEERIDVFFRLAPENK"
     gene            complement(16205..16411)
                     /locus_tag="VLE22_08700"
     CDS             complement(16205..16411)
                     /locus_tag="VLE22_08700"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14526.1"
                     /translation="MLTCPECDSQIDLEEEELDEGDLITCDECAAEFRVVSVDPLELE
                     AIDEDDEEEEDEELDEDEEEDRWR"
     gene            complement(16475..16981)
                     /locus_tag="VLE22_08705"
     CDS             complement(16475..16981)
                     /locus_tag="VLE22_08705"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB14527.1"
                     /translation="MKTMIRVETVLDSWKTVRADTAQAVEDFPPGEFDFRPSPDVMTF
                     REAAQHILLAGHALTGMLLDGVESFATPDFREKLKQYYPPAPDDADGLASELRKSVEE
                     RCVQLVRQPAEFFSGMVTRMDGQEVSRLELLQFVKEHELVHRAQMFLYLRLKGIVPVT
                     TRRRQAKK"
     gene            17106..17927
                     /locus_tag="VLE22_08710"
     CDS             17106..17927
                     /locus_tag="VLE22_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB14528.1"
                     /translation="MSIEVGVNMEFIRHEDKPFAAGVERAAQLGYKYVEPMVHNGREL
                     LSEAGYFHSFSMDDDPGEMKDVLDRCGVKTSGLSAHCPLMRPEISVPYLQKAIRLAAA
                     IGSPVVNTDEGIRPEWVTLEEAWHVMKYTLTAALRVAERYGVYIGIEPHQSISKTTDG
                     LLRIASLVDSPMLRVNYDTGNAFLGGMDPYEGLKAVGKRLVHVHAKDISFQQADSERG
                     KVTGTPVGCACGDGVIDWAKVIGILKEQGFHGVLSVECGTVEQAARSLAHLSPLV"
     gene            18014..19492
                     /locus_tag="VLE22_08715"
     CDS             18014..19492
                     /locus_tag="VLE22_08715"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HSB14529.1"
                     /translation="MIRPKALMAGILITVAGYVTPTPACDTWVALKDATADGSVILVK
                     NSDRPTMEAQPLVQIPRAKHPAGSKVKCTYIEIPQVSETNEILLSKIWWAFGGEHGMN
                     EHGVAIGNEAVWSKEPYQWNDGLLGMDLLRLGLERGKTAYEAMHVMTALLEKYGQSGD
                     AEHPGEWGKANYHNSFIIADPNEAWVLETAGRFWAAKRLTSGFFSISNIYSIENDWDE
                     ASPDLVDHARKKGWTKSAADFNFARDYGDYWTTESKNPGDMQIRRNSTLACLKRDYSR
                     ITPASMMTINRQHHEGTIAAPRWGAAETFWATPCMHDNSRRPYHSAASMAAHLRSGMP
                     PLLRQVYWASFSNPCCNVFKPFYFHGVKIPENYGKGTSTYSADSPWWWANRLKLLCDL
                     NYRGVNPAVRAVFDETEQWQMSRRQAVEAQALRLSEAGKHADAVKVLQAFVDENCSRI
                     EREYGMLQRVAEDMLKTLGVKYLFVDYVRDWTSKKGVPLPLD"
     gene            19537..>19773
                     /locus_tag="VLE22_08720"
     CDS             19537..>19773
                     /locus_tag="VLE22_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14530.1"
                     /translation="MRLTIVFALLAVPLLAQVAPPNEMGVSMGHFHIITPNPDAQKKV
                     WVDVLGGKLLKVGPLDFAMFPGVLMGFRKGEAEGG"
BASE COUNT         3785 a         6039 c         5937 g         4012 t
ORIGIN      
        1 ctctagcgtg tcgctgatct gctcagcttt tctgcccgcc gtcgcaaccc tcgatgccgc
       61 tataagtgcc cgttgagctt cctccgaaaa ttgtcctcgg ccatggcgcg ggtaaactcc
      121 ggcatctccg agtagtgctc aacgtctttc tcgtagaggc tttcccacgc ggctcccgcg
      181 gcccgtagca atgcgagcat ccggttaggg tccgcgcttg agccggaaca ccctgctgtg
      241 ggccttctgg attttggata ttctcatgtt tgacaaaggt gtccttgggc cttcggaccc
      301 tgggaccggt tcagggggtc atgcgcagtc ccgatgctgg tcctttaggg gagaccgtct
      361 tcgacgccag cgttcttgcg attgtggcag cgctcttggt gaacaaagcc ggcggcgggt
      421 gggtcgattt gctcgtgacg cctgcaacct acttcttgag agctttagct gccatacgtt
      481 tacctcacag tgcatttgcc tctcgcaatg ccgaggcagc tatccctttc gcgcggcccg
      541 cgtacgagcc gccttgcgtg cggccttctg cagcccagcc tttcctttag tcctgacagc
      601 ttgctgcgca gcgcggcgcc ggtcggccgc ccctcgcttg cgggcgctcc tgtgggcctg
      661 cctggagaga gctgttcgac tcacggattg ctggccttcc tccttaagag ccgccaagac
      721 cgccttggat cgcttgggcg aggtctgccg cgtgccggac gcttggccct tggcgtagtc
      781 tcgcttggcc tgagcccgcg tctccgcgga tgttcgttcc tccggcggcg ggggcagctt
      841 taccccagct cttctggctt tcgataaccc gattgcgatg gcctgcttgg cagaacgtgc
      901 tccgtgcttg ccttcccgaa cgtgcttgat ttcctcatga acgaattctc cggcttgcgt
      961 ggagggagat ttgccttcaa gggccctctt ccgagcccgc tctcgtgtgg ctttttcagg
     1021 catacgggcc tcctcacgcg tgtctgcgca attttggttc cgtaacgacg tatacggcac
     1081 ttaagtcagc cttccgcacc tcttagcggg tcaaaatgtc gagaagcgca gtaagaattt
     1141 cggagttgat gaagggcgaa gctcgcgaac ggtgagggat tcgcggcgct tctcaagtgt
     1201 gtctagcgcc gggcccggtc gagcgcccgt ttgacggccg gcgtcgggtt cgtgatctcc
     1261 gcggcggacc ggattgtgat cgtcggcagc gccccgttcg cttcatgaac ctgggcacgg
     1321 agttcctcat cggcattgcg gccgctgagg ccggagtatc cgaacgaccg gcacagcttc
     1381 ccgaacagaa agctgttgag cggacagccg cccgtccgtt cgagccaatc cgtcgccccc
     1441 ctcgggtttc tgaacaggtg cacctgcgcg aaggaaagcc cctggaagaa ggtctcgttc
     1501 agtccctccg caccgaccga gagaccgagg ccggcaatct cgtggatcag ccggttcacg
     1561 ccctcagtcg aagtcatatt ctcaacgagg cagttgtgga ggttgaatgt gttgagggcg
     1621 acatcgacga acacgtccct gagcgcgtat tgtctggccg cctcctgtat tctcgaggac
     1681 agtatggagc gccacgtggt caatcccggg tgaatcttga ccatgacctt ctcgcggcgg
     1741 tgattcaggc ggctggcgtt ggattcgggc acgccgatgg cccggttatt gacccaactc
     1801 cagccgtaga gcttcttcgt ttcgatgtcg cggtattgga agtccctgac ctgtgcgtag
     1861 accgggtggt tggggtcggt ctccacgaca ttgaagtgcg gcatcacgtg aaagcccatc
     1921 tcgtgagcac gagccaggaa cgccactgca ctctcgctgg caacgtaggt ggggtagttc
     1981 tcgtcgtagc cgtcggtcct ccaactggag aagtgaatga gaactttctt ggggtccacc
     2041 tgcttcgcaa ggttctcgag gatggcgatg tctcccggac accatgaaat cgccatcgcg
     2101 agcgcatgga gccacggctc gcggcgctgt tcttccctgg cgagcgaaaa cgcctcccag
     2161 agccagctcc ggtagcgctc cgccgggacc ttccagtcgc cttgatggac gttgatcctc
     2221 caggcgagtc ccccggccgc gagattggcg tcgatcggtc cgtacgcctc ggtgtcgagt
     2281 ccgatcatgt aaggatcgtc gtcactgccg acgtgaagcg ccttgtagcg atagcggtcg
     2341 tcttgcgtgt gaaaggaaaa gccaccggac gaggactgga gaatggccag ccccgcctcc
     2401 cagcgatgag gccagtccca gtgggaggag cggatcaggg aatcgtccag gttgagcgac
     2461 acgccttgaa agaacggcgc aaccagcttc aggtcccggc gcagtccctt cagacgccat
     2521 cggcaggcgc ggacgcctgg ccgggaggag aaggcggaag gctcgacgac gagatcgccc
     2581 gaggcggggt ccacggagat cgtgatgaca ccgtcgccgt cccagttctg aaagatcact
     2641 tcagcccgat ggtccgagat ctttcgcgtt tccactttgc cgaacttctg ttcccccaca
     2701 tccaccgtct cgccccgccc gtacacaagc tggagagcgt cgaacttacc ggtgtcgaag
     2761 cccgtgatgc actcctcgga ggaagccttg ctcttgagcg aggtgatgaa tcccttttcg
     2821 agttttgcca gcaacgtggg tgtttcggcg tgaatgactc cgtcactggt gacgctgagt
     2881 tgtgtcttgc cttccagcct gtgcgacaga ctggacgaga cgaaggcggg ggtaggctgg
     2941 gcaagcgctg cggagacgac gccagcggcg cggacgaaac tacggcggga gaactcgcga
     3001 tcggattcca tggccactca gaatatcagg actttggtta cagcggccca cgcccgaccg
     3061 ctcttccttc gttcaggatc tccagaagaa gcttgaggta gtagtagtct ccgaggatca
     3121 tggactcgtc gacgccgagt ccggcaggct tgtgaagagt cgcgtggaca agcattcttc
     3181 cttccgaaac cgcgtcacct gtggccatat agcggttggt cagtgaatcg accgtccgga
     3241 tcgcgatggc acggtattgc ccggcccgca ccggattctg caccaatttg gacagcttcc
     3301 aaaaccctcc ggcagccatc gccgcggcgg aactatcccg cggctcgcgc ggaatccccg
     3361 gggcgttgaa gtcccagtaa ggaacgcagt cctccgggac gttcttctcc cactacgctg
     3421 cgagccgctc ggctgcttcg agccaacgcc tcttgccggt gaagcggtac gcgtcggcaa
     3481 gggcatagat gccgaacgcc tgaccgcgcg accagcaaga ttgaggagag agtccttgct
     3541 tgtgccggcc tgcgactttc tttccggtcc gtgtgtcgag ctggacgact tgaattgaac
     3601 tgccgtctgg acggatggcg gcgtcgacgg tcacgcccgc gtggttatcc gcgatctccg
     3661 aatactttcc gtcgccagtc tcccgcgccg cccaataaag aagatcgaga tcgatgaggc
     3721 agtcgataat gacgaactcc tgctgtttgg tgtttccgat agtgccccac gagcgaatgt
     3781 attttccgtc cggcatgtag cgggccgcca gggaccgggc cgcgtccagc gcaatccggc
     3841 ggtacgctgt gtctccggta aggcggtacc cggcaacaaa tgtcggcatg aacgacaggc
     3901 cgaagttgag atctcgttta tcgtgccgga actcgccgat tgggttcgtc cagcgctgcg
     3961 cgcgttcgag ccatacgggg tccttcgtct gcccatacat gagccagagc atcccggcaa
     4021 agtacccggc cgtccagttg gacggttccc gcaatttcca cttcccgtta acggtgtggt
     4081 caggatactc cgagcccgcg atggcgcaca gccgcccaag ccgggtttcc acagtggaca
     4141 aggcttcccg ataatcagcc ccgctcgcct gcggcacggc gaggagagtc gcaatggctg
     4201 cgacggcgag ggtgccggtg ctctcgcaca atgagaacgg ccgcgaaagt atagacacgt
     4261 ggaataccgc catcattccc atgaaccagg atttctgtct tggagtgctg accacggtgg
     4321 attatacatc cgactcccgt cttgtttcgg cttttcttgc cgaaattgcc gtccggagga
     4381 aggccgtctg gtgtatgctc tgaatagatt cggaagccgg ggtggcgatg ccggctgacg
     4441 cggggtgaca agtccaatgg actatctatt ggaaatggtg caaaagccgt tcgccgaact
     4501 tcggggggaa caacagattg cagcagtcct gattgttctc ctctgtgtct tcatcgcttt
     4561 cagtgcagtt ttccaggcag tgcggggaat gaagggccgc cggcgctatt ggcggcaccg
     4621 gcaaaccggc ccgatcgacg tgtcctggcg gagcagcttg ggttcaccac gccgggagca
     4681 gggacgctgt gtggacatgt cggcaggagg cctcagaatg gaattgcagg acccgatctt
     4741 ggtcggtaca cccgtctcct tccatctact tcagaccaaa cgggcaggaa cggccttcgt
     4801 gcgccactgc acgcccgacg gctcgaagtt ctatatcggg gtcgagtttg cgggctcgcg
     4861 gtgatccaat ctcactgaga aagctgaagg ggcggggatt ccatgcactc tcgaagcctg
     4921 aagatcacgt tcaaaccctc acttcgcgca agagcgccgc gcgtttcaag cgccatctat
     4981 ccggctgttc gcgatcctct cactgctggc accaaggact ggaaagcccg cccctcattc
     5041 ctgtaaggct cgatagcgac ctccaatcgt aaaggcgcat ctttcaacgc cgtcttcaga
     5101 acccgctacc gctgggttcc aagtttatcc tactccagtt ccgccgcctg tcaatagtac
     5161 gttttgtcca tctatggcag gaatctgggt ttcggccgcc gccggcagga tcgaaattgt
     5221 tacacggcgc cggtttgtct ccccggtttg ggggtgagac actgttgctt gtcgctcgat
     5281 gtacgcgcgg gcgagaggag actcctgcat gaaaaaggcg cttctgtttg ccggcgcagc
     5341 agtgatcacc ctgctcgccg ttgccgccgt gtatgtctcc cggctcaatg tcgacaggtt
     5401 ccggccccag atccaggctg aactccagcg acaactgggc cggcaggttg cactgggcgc
     5461 catgggcgtt cggatttttc ccctctccat ccggatcaat gacgtaaccg tcgcagagga
     5521 tccctccttt cggacggacc ggccgtttgc ttccgccagg gaggtaagcg tggctgtaag
     5581 cctatttccg ctgctgcgga aggaagtcca cgtccagtct ttcgtgcttc gagacccggt
     5641 gatcgaactc gttcagaacc gatccgggaa atggaacttc tccagccttg gcgccggcgg
     5701 cggaggcgag ggcggcggac tatccgtcgc ggagctccgt atcgaaaacg gaaggatttc
     5761 ggtgagcgag ctcgcggata gaaagcccgc ctcggtgtac gaccacatcg gcatcaggtt
     5821 ggccgacttc gggccgggca aggtctttcg cctcaatgcg tctgcccaac ttccaggggc
     5881 gggcaaggac gtactcatcc tcgaagcacg cggtgatcct gcgcatgcac aaggggcact
     5941 gctggccgga caagtccaga ttcagcaggc gccgataagc gccctgcgca gcttcctaca
     6001 caccggggcc acaaaggccg atggcagcct ctccacggcg atggacttcg agctgcagaa
     6061 ctccgcgctg aaagcaaacg gttgggtcga actcgcgaat ctccgcaccg gcggagccga
     6121 tctgggccgc cctatccggc ttgactgcaa gatcagccac aacctggata cgggagtcct
     6181 taaggcctcg gaggttacgg ctaggatagg caacgctcct gtcgtgattt cgggagaact
     6241 ggacacccgg gcgtccagaa tcgacgcgac gatcaggacg gctggagccg gcctgaagga
     6301 ggtccttacc gctgccagcg cattcggcat aagcggcatc agcggcaccg ggcagctcac
     6361 tctcgacgct cgcatccgcg gaccactcga tcgcatcgcg gagctcgagt gcacgggccg
     6421 agcgagcatt cgggacgcca cgctaagtgt accggccctg acgaagccac tccaggtgcg
     6481 ttctgccgac ctcgatttcg ctgggagatc cgcaggtttt gaaaacctgt cctgctccct
     6541 aggaagttcc actctgcggg gaaacgtgat ggtccggaat ccggcttctc cggaagcgga
     6601 gttcaacttc gacattgacc ggctggatct cgccgagttg cggcagttga cggctacgaa
     6661 ccagccgagt cgcagcgagg gcggccgatc ctcgggcagc tttacggcga aaggaagcgt
     6721 gcacgtcgga acgatcgctt acaaccagat ggtccttaaa gacgtacgct ccgagtgcgt
     6781 tctgagccga gggatcctga cactgtcccc gttgacggcc gagttgtttg gcggaaggca
     6841 gagcggcagt attaccgtga acctgcaatc gcagccgccg accgtagcgc tcaagacgaa
     6901 cctcgacggc gtggatgcca atcaacttct ttccaccacg acatccctga agaacatgct
     6961 ttatgggtta ctcgccgcca aaggggacgc cgccatgcag ttgtcctccg ggggcgacgc
     7021 tgcgcgcacc ttgaacggga acttgaatat tcagctcagt aagggcaagc tcggtggcat
     7081 caatctgatg aaccagatgg cgagcctcgg aaaattcgtg gggtttgccc ggaatagtga
     7141 gccgttcacc gacattgttc agctctcggg cgacatccag gtccgcaacg gagtcgccga
     7201 cacaaacaat ttgctcatcc agaccgaagg cgccagtgtc tcgtccgccg gaaccttcag
     7261 ccttgtcgat cagtcgctca acctccgtct cacggcagtt ttggaccgcg acgcgagcca
     7321 gaaagcggga ggctcgcgag tcggaggatt tctcacgaca gctctagtca atggaaaggg
     7381 cgagctggtc attccggcga ttgtgaccgg gacgttcgca aatcctcgat tcgccccgga
     7441 cgccgcgcgc atcgccgaga tgaaactcaa gaacctgatg ccgtccactg ctaacccggg
     7501 gtcgatgaca tcgggaattg tgggcattct cactgggaag agcggcggcg agcggccggc
     7561 tcaaggcacc acgacgagcc agcaggaaaa cgttgagccg gcgcaggctc cgggggcgaa
     7621 accgcccgag gcgcctcagc gcaaaggggt gctcggcatt ctcgactccc tccgcaagaa
     7681 ggcctcggag ccagcgaaaa agaacccctg acggggtatc ggtccgctta tcggcggctc
     7741 gctggcggaa tcacgctatt gagccgcgca taaaccgcca tctggccgta atggtcccac
     7801 gtgtggccga tcgccgctga aaccatcgaa gctctcggca cctggaagtc gccgtaggcg
     7861 gatttcacca ttccggtgag attctcgctg gttacactgc tgaccgcttt gcggacgtac
     7921 ccgaaagcat ccttcagata cttcacggcg tcctctttcg tcgcgagtga tgcaggaccg
     7981 ttgtcgttcg ggcccggttc gcttgggttg gcctctccca agatgctggc gccatacatg
     8041 tacaacaacg ccgcggtgtg cgtcacctgc tggtggaacg tgcgcgcgtt ccggaacgcg
     8101 cccgtgcccg ggacaaaccc gtatttcgcc gcgggcatct cctccgcgag cgggacgatc
     8161 tcacgctcaa tcaccgaaat ctcctggtcg aagatcttag cgatcgacga ggtggccgga
     8221 tccgcgccca agagggcggc agaggtgaca gcgaacatcc acagtacacg gcttctcacg
     8281 agaatcctcc agattgcgga tttccttgat tatccagaat ctctacccgg gtgtctacgt
     8341 cgattgtatg gtgcaacatc atgagaaatc aacaacttaa tccccacctg ccggaacgca
     8401 aatgcggatg attccaaccc agcgctctcg gcggccggct ctgcggaaaa gaattgtgca
     8461 ttccggaaac gcggaacttc atctactcat ctaaatgaca ggtctgggta tgccaagcaa
     8521 accgattttc cgccagttgt ccgttctcgc tctcactctt ctggtttttg gaggcaatgt
     8581 gacacctgca gacaagggat cgtttcccga gcccgccgtt gacgtcaagc ccgctaccgg
     8641 caagcagact gccgtcctcg ccggcggctg cttctggtgc atggaagccg tgttcgaact
     8701 ggtggaaggc gttgacgacg tgatctcagg ctattctggt ggcactaaag agacggcgaa
     8761 gtacgacatc gtttccatgg gacgcaccgg ccacgcggaa gccattctca tcacctacga
     8821 tcccagcaag acttcctacg gccaactgct caagatctac ttcagcgttg cccacgaccc
     8881 cacaacgctc aaccgccagg gccccgatac cggcccccaa taccgcagcg ccattttcta
     8941 tgccaacgag gagcagaagc gcgtcgccga ggcatacatc aagcagctca acgatgctca
     9001 tgtcttccgc aacccgatcg tgacgcagct cgtaccgctc gacaagttct acacggccga
     9061 ggcctaccat cagaactacg cggtgcagaa tcgttccaac ccctacatcg tgaacgtttc
     9121 tgatccaaag gtccaaaagc tcaagaagat gtatcccggc tgcgtccgca aaaggaaata
     9181 accatgaaca ggagaacttt tctgatgggc ctgtcttctt cagccttctt gctggggaaa
     9241 ggaaaaaccg tgaagattgt tgaattctct gacaccggcg agcgcaaagg cttggtcgaa
     9301 gtcgagaaga tcgtcaaaac cgacgaagag tggcgaaggc aactctcacc cgccgaattc
     9361 gaggtcaccc gcaagggagg caccgaacga gccttcaccg gcaagctgaa caagaactac
     9421 gcggagggca tctaccgctg cgtctgctgc ggcacagccc tgtttcattc gaagaccaag
     9481 ttcgattccg gcaccggctg gcccagcttc ttcgccccca tcgccaaaga gaatgtagtc
     9541 gagaagttgg acgctaccct cggcgtgacc cgaatcgaaa accaatgcgc ccgctgcgac
     9601 gcccacctgg gccatgtctt cgacgacggc ccccgcccga cgggacgccg ctactgtatg
     9661 aattccgtcg cccttacttt cgtcaaggcg cactgatgag atctttggga agcacggtac
     9721 cgtgtgttga cgaggctctt acgccatcgc caggatcgtg tcgagttccg ctgagagctt
     9781 tttcagttgg tcgggatcat cggggttgta gccgtactcg ggggagatga agccccggta
     9841 gttgacctgg accagcgccg acatcacggc cttccagtcc acatcaccct ccagcagtcc
     9901 tcccggcgga ccctgcggac cccgcagtgt tgccctgcgg ttctttgcgt gcagccgctt
     9961 gatcctcggt cccagcgtca ggatccagtc ctgtgggaag ccgtgcgtca ggatgtttcc
    10021 gatgtcgaag aagaaactca accatgggct gcggaactga tcgacaaagg aacgcgcgtc
    10081 gagcgggctt accagaaaac gattgcccac gttctccact gccatcacga ccttggcttg
    10141 ctcggcgtaa gggagtgcct tcctgagctc ggctgtaatg cgttcatagg tgacctcata
    10201 gccttggaag aacttgttgc cgtatcccac gtaaccgggg acgagcagca ggccatcaca
    10261 gttcagatac acggcgaact cagtcgcttt ccggattgct tcgacaccct tcgcacggac
    10321 ttccgcatcc gggtgattca ggggattctc tccgagggga gagacccaca gcgcggcgat
    10381 cgcaacgcct gcctttttag cctgatcccc gatcgctttc acctggtcgg ctgtggcggt
    10441 tggcacgaat tcgtccagca tcgggacctc gatcgcgtcg aagccggcgt tcttagcctg
    10501 ccggaaacat tcctcgatcg gcatctgctt gggaaaattc acccgacaga tgctcttgat
    10561 aaaacgacct ccggaagtct gtgcgtgcgc gagtccggcc agcgcaccac tcgccgcggc
    10621 ggcgaattgt cttcggttga tgactgtcat agaacacgct cctggcttac agaaaatcga
    10681 cgcccaattc cttccatgtc aggatcttct tgcgattgat cgcctcgcgg ccgagaatcg
    10741 acgtcaacgc gccggaagct cccacgatga tgtcagccgc cggtttcttc ccggtgcgga
    10801 tgcactcata gaagtcggcg atgtgcctgt cccggggcgc cctctcctcc ggcggtgcta
    10861 acacctgcgg ctcaccgcct tcgtgcggat aaaacatcgc cgtgtccaga tccactgcgc
    10921 cctttgtgcc atagataaac gtgtgctgcc acgcgtaggg catcttggcg ggatggaagg
    10981 cgacctgcgt gaaagccagt ttcaccccgt tggcgtattc gtagctcaag gtgtagccgt
    11041 ccatgttggt ccggccgggc ggatcgttca cgtacatgat gttcccgccg aaacctgtcg
    11101 cccgctcagg atagctgttg atcgcccagt tgcagacgtc gaggttatgc acggagttct
    11161 cggtgatcag gtcgccggag cgattgggga agaagaacca gtccgctgaa gagccgtcgt
    11221 gcgcgaggtc gctatcattg tggcgttgcg ccttgacgta gatcaccttg ccggccacac
    11281 cctcatggat gcgggagatc gtgccgcgca gccgcggctc cgaacgcctc tgaagtccgg
    11341 tttggaagac cgtcttcgaa ctccgggcca ccttcaccag ctcgcggatc gattccgctg
    11401 taataccgat cggcttctca caatagacgt gcttgcccgc ctttagcgcc gcaatcgcca
    11461 tctctacgtg gaggtcgcac ggtgtagcga tgaaaaccgc accgatgtcc ttacgatcaa
    11521 tcagcttgcg ccagtccgag aatgtcgtgg gctggtcgcg cgcggcgatg gtggctgccc
    11581 ggtccagacg gtccggcttg atgtcacaaa cggcaacgac cttcgcggtg ggttcgtcca
    11641 acgtcagttc caggagatgc gaccctcggt tccctgtgcc gatgaagccg gtaccgatcg
    11701 cgccggagtc agcagcggac acgctgggcg ctgccgcggc catgcttccg attagaaagt
    11761 gcctacgatt cattcaggga tgatcctttc ggtgggattg tataacatcc tcgggcgcca
    11821 ctccgactgg aagcgcggtt atcagacgtt gtcgggctcc ggatggaccc aggggctgcg
    11881 gtagggccgt cgcagtaggc ggttggcttc cgagtcgccc aaaaccgtat ggtttacggg
    11941 gtcccaggcc agcgtgcgcc cgagttgcat cgcgagattg gcgagaatgc aactcgtcgt
    12001 cgagatgtag ccctgttcga tgtcggcgac cggcttgccg cgcgaggcga tcgccgacag
    12061 gaagtcctgc atgtgatgcc ggatggccgg agcgacgtgg cgttccagcc gttcctcggt
    12121 cttgtcttcc gggtactgct ccagttcgta cgtgacgtcc cggtgaatcg ccggatcgtc
    12181 cttgcccatg ggtgtgaagt cgtagctcat cacgctcgcc ttgagcgtgc ccttctcgcc
    12241 ataaaacgtc gcgccccacg ggtacttggg atcgggagga tggccccaac tccggtgctg
    12301 ccacaccacc gacatgtcac cgtaatcaaa cgtggccgtc tgcgtgtcgg agatattcgc
    12361 tttcgtattc ttctgtacca ggatccctcc cgacgaagaa atccgttttg gccagccgag
    12421 gcccagcatc cagcgcacca tgtcaaacat gtggatgcac atgtccccga cgatgccgtt
    12481 gccgtactcc atgaacgcgc gccagcgtcc gggatgcacg agttcgttgt aggggcgcat
    12541 tggggcggga ccggtccaca tgtcgtagtc cagatacgcg ggcgggtttg tgtccggcgg
    12601 attgccggtg gcgcgcatgt ggtagtagca atagatctcg accagcccga tcttacccag
    12661 cttgccctcg ttgatgatcc ggtcccgggc ctcggcgagg tgaggcgtgc tacgccgctg
    12721 cgtgccgacc tgcaccacgc gcttgtattt ccgggcggcc gccagcatgg cgcggccctc
    12781 gacgatgtca acactgatcg gcttctggac gtagacgtcg gcgccagcct ggacggcagc
    12841 aatcatcggc aaggcgtgcc agtgatccgg cgtggcgata aggacgatgt ccagatcttt
    12901 ttcagccagc atccggcggt agtcggggta cgttcgtggt ctccttttcg atgcctgccg
    12961 ggcggccacc cggtcggcgg cgtcggccag catcctcgag tccacgtcgc aaagggacac
    13021 cacctcgacc ggcgccacct gaatgagccg gatcagatcg cactttccgt accagccggg
    13081 accgatcaat ccgacgcgtt ttggcttctc ggcagcgacg gccctggcaa aactgccggc
    13141 agcggcagtg tgaaggaatt ggcggcgatt catctttgca ctctcctggt tggaaccgaa
    13201 cctctatggg gcagaaggaa aagtataatt ccttccgggc gcagtaggtg gcgcctcggg
    13261 ggaagcgtta caatcgccgc atgccaacga gactggtttt gatgtgcgtg ctctgcacgt
    13321 ttgtggtctc cgcgcaggcc tccgcggggt ggcgaaaggg ccaaggctac ggatgggtgt
    13381 acggaaaaca ggacgaggtc ggcgcactca acaccgtcac cggtcccgag catgtgctta
    13441 aggctttgcg cgccgtgaag accggccggg ttttcgacct cggtgtgccc ctcgatcgcc
    13501 ggtcctacaa atggccaggg cactcgccga ccgaggtcat gtcgtttcgc agccccgaag
    13561 gcctcaagcg cggcaaggat ctcggcccgt tcgcggccga cccgaaaggc ctgggcttcc
    13621 acagttgcgc actgttcatc tcagacaatg taggcactca gatcgacggc ctcggccaca
    13681 tcactaccgg cgcagacaac cactggtaca acggattccg cgaaggcgac tacggcacag
    13741 acttcggaat ccgccgggcg gacgccgact ctatcccacc cgtcatcgcc cgcggcatcc
    13801 tcatcgacgt cgccggctgg aaaggcgtag gcgcgctgcc ttcgaacttc gcgatcggct
    13861 ccagggagct tcaaagcgcg ctggcggcac aggggacgga cgtcgatccc ggggacgttg
    13921 ttctgattcg aactggcgtc ctgcgctact ggggagaagc gggggagaac catgcgaaag
    13981 tcgcggaaca cgattcggcg ggactgacgc tggagggagc caagtggctg gtcgaacaga
    14041 aaggcgccgt catgatcggc gccgacacca gcggtcttga ggtgggagct gacccggctc
    14101 taccaggcgc gagtattccg gtgcatgtgt acctgctcgt cgagcagggc gtgcacatcg
    14161 gcgagcttca ctacctcgag ggactcgccc gcaacaaggt ctaccgcttt acgtacgtcg
    14221 cgatgaccaa ccgcgtgaag ggtgccgtgg ctggtttcgc catgcgcccc ctggcgatcg
    14281 agtagatcgc gaagtcagcc tgcaagcaac ttgcggatcg ccttctccag ggcggccata
    14341 tcggtctcgc caacgaacga cttcaccagt cggccgttcc ggtcgtagag aaacaagccc
    14401 ggtagtgctc cgctccactt cgggtcgacg gagttgatga acttctcatc atcgtcgacc
    14461 cgtttcaggt aacccagcgg ggctttgtgc tgtttcagta cttccagcgc cttgttcgtc
    14521 tcttccggct cgtcgcagga gacggtgact acgctgaggc ccttaccgcg gtactttgcc
    14581 tcgagtttca ccagaagcgg gagttcttcc aggcaggggg aacaccatgt cgcccagaag
    14641 tccaccagca gcacttttcc cttatgggac gcaataaccc ttttgtaggc cgcttcgtcg
    14701 agcggagtga gtttctgctg cgcggcgagc gtggccgccg ccgcaaatag gatgacagcc
    14761 gtcagaaggg cgcggctcaa gacgctttct tcactcgctt gatcgtgcag ccgaaggcct
    14821 tggtctccgc acgttctacc ggcttgcccg ccaggacgga gtcaagcgcc attcgcaacc
    14881 cttgcgtctt gatgttggcc gaattccggg agtcgtcgat gtagccgtga tactgaatgg
    14941 tcccgttctt gtccatcacg aagatctcgg gggtcacctg ggcgccgaag cggtccgcga
    15001 caacattgtt ttcgtcttta tagaccttaa acgaaaactt gtgttcagcg gcgtgctgtt
    15061 ccacctccgc cggcggttcg ctgctgttcg cgttgacggc gacgaacttc acacccttgg
    15121 gggcgtagtc ggccacgacg gcgttcatgc gctcgttgta atcgttcgag atcgggcatt
    15181 gcgtggcgat gaacatcagg acggttacgt cacccttcag ttgcgagaac tggaccttgt
    15241 tccccttcag gtccgagaga ttgaaatcac cgaccttcga cccgagcttg aattcctggc
    15301 cgaataggac ggccccgaaa gacgctgtga gccccagcca caacagtcgt ttcattgtgt
    15361 tcctccagtg agaagatccc ggccgggcgg ccggtgttat tttgattcca gcttgagatt
    15421 gataatagct ttcttgcgcg agtcgaacac gctgagcgtc ttggcctcgc tcgaggcccc
    15481 cccgcgatcc gcctttagct catagtcaat gttggtgctg aggccgtgga aataatacgt
    15541 gccgtccgat tgcgtgatga aggagcgcac ctgaagggac ttggtattct tcagcttcac
    15601 aacggcgccc tcaacgggac tgtccttctg gtccactacg acaccctgga cggagcggac
    15661 gttcggatcc tcgcgctgct tgcccttctg caacgccagt cctgaggcgg tggcccataa
    15721 gaggatgaca accgcaacga aaatcgcttt tgtcttcatg tggacctcga ttatatttag
    15781 atgcgccgtg ctccggatag ttacggttta tttattttca ggagcgaggc gaaagaacac
    15841 gtcaatccgt tcctcgccgc cgatggatac ggacttctcc tgagcgcgaa accccggtgc
    15901 gcttacgctg acagtataac gcatgggctc ggccggcacg cgcacgacaa actcgccgcg
    15961 gctgtcggaa accaccttta ctttcttgaa cttatgagag gatttcgctt gcgggtcacg
    16021 gctgattgcg acttccgcgc cccgcagaag gaagccggac tcctggaata cggtcccggc
    16081 gatcattgcg acggcgacct gccgtttctg gtccttcgcg ggccgcccgg cagccgccgg
    16141 cagtggcaga caaagcaaga tgcaggcaac tgcgaggatg ggatagatcg gggcctcgga
    16201 ccgcctacct ccagcggtct tcctcctcgt cctcatcaag ttcctcgtct tcctcctcct
    16261 catcgtcctc gtcgatcgcc tccagttcga ggggatcgac gctcacgacc cggaactccg
    16321 cggcgcactc gtcgcaggtg atcaggtctc cctcgtcgag ctcttcttct tccaggtcga
    16381 tctggctatc gcattcgggg caggttagca tcgagcatcc tccaacagcg ctgcacaacc
    16441 tctttatacg aaactcccgg gaatttgcca acagctattt cttggcctgg cggcggcggg
    16501 ttgtgaccgg gacgatgcct ttcagccgca ggtagaggaa catctgcgca cggtggacga
    16561 gttcgtgctc cttcacgaat tgcaggagtt ccagacgcga cacctcttgc ccgtccatgc
    16621 gggtgaccat cccggagaag aactcggccg gctgccgcac tagttgtacg cagcgttctt
    16681 ccacgctttt tcgaagctcc gacgcgagac cgtcggcgtc gtccggcgcc ggagggtagt
    16741 actgtttcag cttctcgcga aagtccggcg tcgcgaagct ctcgacaccg tcgaggagca
    16801 tgccggtcaa tgcatgaccg gccaacagaa tgtgctgcgc agcctcgcgg aaggtcatga
    16861 catccggcga gggccggaaa tcgaattcgc cgggcgggaa gtcctccacc gcctgcgctg
    16921 tgtccgctcg gaccgtcttc cacgaatcca aaactgtttc gaccctgatc atcgttttca
    16981 tttgtaccat ggcctgccgc cccgaaatgt cctgcagggc gttgaactgg attcagaagt
    17041 agaaggtggg ataatctctt gttcggccgt ttgccatccg gtcgccctta aggaaaggag
    17101 cagtcatgag catcgaagtt ggcgtcaaca tggaatttat ccgccacgag gacaagccgt
    17161 tcgccgccgg cgtggagcgc gcggcgcagc ttggctacaa gtatgtcgaa cccatggtcc
    17221 acaacggccg cgagttgctc agcgaggcgg gatacttcca ttcgttctcg atggacgacg
    17281 accccggtga gatgaaggac gtgcttgacc gctgtggtgt caagacttcc gggctgagcg
    17341 cccactgccc gctcatgcgt ccggagatca gcgtgccgta tcttcagaag gcgatccgcc
    17401 tcgcggctgc catcggctcg ccggtagtca ataccgatga aggcatacgg cccgagtggg
    17461 tcacgcttga agaggcctgg cacgtgatga agtacacgct caccgccgct ctgcgcgtcg
    17521 ccgagaggta cggtgtctat atcggcatcg agccgcacca gagcatctcg aaaacgaccg
    17581 acggtctgtt acgaattgct tctctggtcg actcgccgat gcttcgtgtc aactacgaca
    17641 ccgggaacgc ctttctcggc ggcatggacc cttacgaggg attgaaggcg gtcggtaagc
    17701 gtctggtcca cgtccacgca aaagacatct cgttccagca ggcggactcg gagcgtggaa
    17761 aagtcaccgg cactccagtc ggttgcgcct gtggcgacgg cgtcatcgac tgggcgaagg
    17821 tgatcggaat tctgaaggaa caaggtttcc acggcgttct ttccgtggag tgcggtaccg
    17881 tcgagcaggc cgcccgcagc ctcgcccacc tcagtcctct ggtgtgaggg gagggggcaa
    17941 cgttcgagcg gcggaacaga ccagcaacag cggggcgacg gaccgagcta tattcggcct
    18001 aaaggggtgc agcatgattc gaccgaaggc tctcatggcg ggaatcctca tcactgtcgc
    18061 cggatacgtc acgccaacac ccgcgtgtga cacctgggtg gcgttgaagg acgccaccgc
    18121 cgacggctcc gtcatcctgg tcaagaacag tgaccggcct acgatggagg cacaacccct
    18181 ggtccagatt ccacgtgcta agcacccggc gggctccaaa gtgaaatgca cgtacatcga
    18241 gatcccgcag gtgagcgaga ccaacgagat tctcctctcg aagatctggt gggcattcgg
    18301 cggtgagcac gggatgaacg aacacggcgt cgccatcggc aatgaggcgg tatggtcgaa
    18361 ggaaccctac cagtggaacg acgggctcct cggaatggat ctgcttcggc tcggtctcga
    18421 gcggggcaag accgcgtacg aggcgatgca cgtgatgacg gctctgctcg agaaatacgg
    18481 ccagtctggt gacgccgagc atcctggcga atggggcaag gccaactacc acaactcgtt
    18541 catcatcgcg gacccgaacg aggcctgggt actggagacc gccggccgct tctgggccgc
    18601 taaacggctg acgagcggct tcttctcaat ctctaacatc tactcgattg aaaacgactg
    18661 ggacgaggcc tcgcccgatc tggtcgatca tgcccggaag aaaggctgga cgaagtccgc
    18721 ggcggatttc aatttcgcac gggattacgg cgactactgg accaccgagt cgaagaaccc
    18781 aggcgacatg cagatccggc gcaactcgac actggcgtgc ctgaagcggg actactcgag
    18841 gatcacgccg gcgtcgatga tgacaatcaa ccgccagcac cacgaaggga cgatcgccgc
    18901 gccgcgctgg ggcgctgccg agaccttctg ggcgacgccc tgcatgcacg acaactcgcg
    18961 acgtccttac cacagcgcgg cgtcgatggc cgcccatctc cgttccggga tgccgccgct
    19021 gctgcgccag gtctactggg cgagtttctc caatccgtgc tgcaacgtgt tcaagccttt
    19081 ctactttcac ggcgtaaaga tcccggagaa ttacggaaag ggaactagca cgtactcggc
    19141 cgattcgccc tggtggtggg caaaccgtct gaagttgctc tgcgacctga actacagggg
    19201 cgtgaaccct gccgtgcgcg cggtgtttga cgagacggag cagtggcaga tgagtcgccg
    19261 tcaggcggtg gaagcacagg ccttgcgcct ctcggaggcc ggcaagcacg ccgacgccgt
    19321 gaaggtgctc caggccttcg tggacgaaaa ctgctcccgc atcgagcgcg agtacggtat
    19381 gctccagcgc gtcgccgagg acatgctgaa gaccctcggc gtcaaatacc tcttcgtcga
    19441 ctacgtcaga gattggacgt cgaagaaagg cgttccgctt ccgctcgact gaacgctgta
    19501 gtagacggcg ccagccgtgg gataatcgcg ttcatcatgc gcctgacgat tgtcttcgca
    19561 ctcctcgctg ttccgctgct cgcccaggtg gctccgccaa acgaaatggg tgtttcgatg
    19621 gggcacttcc acatcatcac gccgaacccg gacgcccaga agaaagtctg ggtggacgtg
    19681 ctcggcggca agcttctgaa ggtaggtccg ctcgacttcg cgatgtttcc cggcgtcctg
    19741 atgggtttcc gcaagggtga agcggaaggc ggt
//
LOCUS       DATIFQ010000189         5653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1404896, whole genome shotgun sequence.
ACCESSION   DATIFQ010000189 DATIFQ010000000
VERSION     DATIFQ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404896"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2136
                     /locus_tag="VLE22_08725"
     CDS             <1..2136
                     /locus_tag="VLE22_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSB14531.1"
                     /translation="SPRGAPVTPSGQREANLTIGDTLITTAPVLKPILADHRAWIASK
                     NLENYTQQVKMAILDTGFDTASDDGVPASFHPDFRMSDGSGTFVTVKRYTTGPGLNAD
                     CSGHGTFVAGVLAGNAGVPLISTETRDCPYPCSDAPYLMGVGVVPEIPLVVGRVFNYL
                     ASNPTPRGFVNQDFTTIFSDLASQGVAITSNSWNRANDTDYDEDTEKLDKLVRSASGS
                     NNGPPMAMYFSAGNRDDATNGNQYVSWPAIAKNVITVGASENFNPSSYTDYPPSNGYA
                     NADNGNEIWIYSQQGPTRPAGVDNRIKPDLVAPGSGMESPATRNDAFGTNSCCIGSCT
                     LPTETVRPVGAVIDSTSGQGHLWSRGTSFATPAAAGAGALLYTWFRNKNVTPRPSLLK
                     AAQISLARPLTGTGQLPPGDKQGWGKADLTRLPRSSQPSDPYEWNNEDTVVNPFMTAT
                     LPSPGGSYRILDATQPVRITAVWTDAPGDPGAGRELVNDLDLTVRIHGAAGYFALGNN
                     FAASGRSTIFVTGVSSGTPDTRNNVEQVVFLAAEAGDSMQFHVELFGREIMKDGIGVW
                     PPTGPLQQDFALFIENAVAVPAGAAVFNTVAPCRVLDTRDPDGPYGGPALTGNTIRSF
                     ALWNRCGISPTAKAVSVNLTTVIPDADGYITAFPGGTPQPNVSTMNYRTGRVRANNAI
                     LSLGVTGQISVFASAGTTHFVLDVNGYFD"
     gene            complement(2170..4302)
                     /locus_tag="VLE22_08730"
     CDS             complement(2170..4302)
                     /locus_tag="VLE22_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14532.1"
                     /translation="MRVPFVANAGQADPAVAFSASMLGGTVFVTRAGQIVYAFPGAKE
                     KQANFSAATRERQLRPGWALTESFLDGSPRPRAGAQAAAKVSFFLGTDSKKWRTGLAT
                     HDSVELGDVWPGIEVSVRARVGSVEKFFVLLPGTPADRIRLSVDGAQDLKIGKSGTLS
                     ALTGLGEVTFSAPVAYQEKDEARAPVAVAYRVKGVEYGFSLGAHDLALPVVIDPLLQS
                     TYLGGASGASVHDIAVHPASGELLVAGSTGNDFPGTAGGAQPAGSGAFVARLNSSLTR
                     LIQATYVGGSGFQQADSLAIAPGSGEVLVAGMTNSTDFPGTGGGAQSVLAGEDDGFVA
                     RLSPTLTALLQATYFGGDQTENLIALTVHSASGEIFLAGTTSSTNLPGTAGGAQPAYA
                     GGDADGFVARFNPTLTALLQATYVGGSALDATTAVAIHPSGSELFLTGISESTDFPQT
                     SGGAQEIQRGRDSFVARFNTALTTLLQATYFGGSGIDDAQDLAILPTSGEILLAGITG
                     SSDLPGTAGGAQPAIRSGLSDGFVAKLNPSLTALLQATYLGGTESDGGIDVSIFPGSG
                     DILVAGFTSSRDFPGTAGGAQPGFGGPSGDAFAAKLDPGLTRLLQATYLGGSSTDTFR
                     AAVIHPLTRDVYLGGYTSSFDFPGTAGGAQETRRAIFSDAFVARLSPDLSSADGQPGA
                     IPALSFPALALLAMLLASAGVLLLKRIG"
     gene            complement(4587..5450)
                     /locus_tag="VLE22_08735"
     CDS             complement(4587..5450)
                     /locus_tag="VLE22_08735"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HSB14533.1"
                     /translation="MKRIVLFAAFLAVFASCRRDGDRALRIGSKNFSEQVILGEIAAQ
                     GLEARGIRVDRRLNLGGTFVCHQAITAGELDLYPEYTGTAFTAILKRRPVSDPAAVRA
                     GVADAYRRWKLVWSPPLGFENTFALVVRGDDARRLGIRKISDLAAHPEIRPGFGYEFL
                     EREDGFPGLAKTYGLDFRDRPVQMDLGLLYPALESGKVDLVAGNSTDGLIDAIGGVVL
                     EDDRHYFPPYEAAFVVRDAVWKRPEVREVLESFAGKIDAATMRKLNAAVDKEKRRPED
                     VAKEFLASVLR"
     gene            complement(5447..>5653)
                     /locus_tag="VLE22_08740"
     CDS             complement(5447..>5653)
                     /locus_tag="VLE22_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine ABC transporter permease"
                     /protein_id="HSB14534.1"
                     /translation="AGVRIATVVSIGLATIAAAIGAGGLGVLIYRGVAIVDHRLILAG
                     ALPAALLALAADAGLGFVEKRLGR"
BASE COUNT         1144 a         1812 c         1736 g          961 t
ORIGIN      
        1 agtcctcgcg gtgctccagt aacgccgagc ggacagcgag aagcaaacct cacgatcggc
       61 gacacgctga tcacaaccgc cccggttctg aagccgattc tcgcggacca ccgggcatgg
      121 atcgccagca agaacctaga aaactacacg cagcaggtca agatggcgat actcgacacg
      181 ggcttcgata ccgcctccga cgacggtgtt cccgcctcct ttcatcccga cttccggatg
      241 tcggacggca gcggcacgtt cgttactgtc aaacggtata cgaccgggcc aggtctcaac
      301 gccgactgca gcgggcatgg gacattcgta gcaggagttc tggcgggcaa cgcgggcgtg
      361 ccgctgattt ctacggagac tcgtgactgt ccctacccgt gttccgatgc tccctatctg
      421 atgggggtgg gcgtggttcc agaaatccct ttagtcgtag ggcgggtctt caactacctc
      481 gcatccaacc ctacaccccg cggattcgtt aaccaagact ttacaacgat tttctcggat
      541 ctcgccagtc agggtgtcgc gatcaccagc aattcgtgga accgtgcgaa tgacacggac
      601 tacgacgaag ataccgaaaa actcgacaag ctcgtgcgct ctgcaagtgg ttccaacaat
      661 ggcccgccaa tggcaatgta tttctcggcg gggaaccgcg atgatgcaac gaacggaaat
      721 cagtatgtgt cctggccagc gatcgccaag aacgtgatca cggttggcgc gagcgagaac
      781 ttcaacccga gcagttatac agactatcct cccagcaacg ggtacgccaa cgccgacaac
      841 ggaaacgaga tctggatata cagccagcaa ggtccgaccc gccctgcggg tgtcgacaat
      901 cgcatcaagc ccgatctcgt ggcgccaggc tcagggatgg agtctcctgc gacgcgcaac
      961 gacgccttcg gtacgaactc gtgctgcata ggctcgtgca cgctcccgac ggaaaccgta
     1021 cgcccggtgg gtgccgtgat cgactcgacg tcgggtcagg gacacctctg gagccgtggc
     1081 acctcgtttg ccacacctgc tgccgccggc gcgggggccc ttctctacac gtggttcagg
     1141 aacaagaacg tcacaccgag accgtcgctt ctgaaagcgg cgcagatatc gctggcccgt
     1201 cctttaacgg gaactggcca actgccgccc ggtgacaaac aggggtgggg aaaagcggac
     1261 ctgacgaggt tgccgcggtc ttcgcagccc agcgaccctt acgaatggaa caacgaggac
     1321 acggtcgtca atcctttcat gacggccacg ctcccgtcgc cgggcggcag ttatcggatc
     1381 ctcgacgcta cccaaccagt ccggataacg gcggtctgga ctgatgcacc tggagatcca
     1441 ggagcgggta gggaactggt caatgatctg gatctgaccg tcagaatcca cggcgccgcg
     1501 ggttactttg ctctcgggaa caactttgcc gcatctggca ggtccactat cttcgtcact
     1561 ggcgtaagca gcggaacgcc cgacactcgg aacaacgtcg agcaggtcgt cttcttggcg
     1621 gccgaagctg gtgacagcat gcagtttcac gtcgaactct tcggcagaga gatcatgaag
     1681 gacgggattg gcgtttggcc gccaacgggg cctttgcagc aggacttcgc actcttcatc
     1741 gaaaacgcgg tcgccgttcc agctggggcg gccgtgttca acacagtggc gccatgtcga
     1801 gtgcttgaca ctcgcgatcc agacggcccg tacggaggtc ccgctctgac gggtaacacg
     1861 atacgctcgt ttgccctgtg gaatcgatgt gggatctcgc caacggcgaa agcggtctct
     1921 gttaacctca ctacggtaat cccggacgcg gacggataca tcaccgcctt tccgggcggc
     1981 acgcctcagc ctaacgtgtc aacgatgaac tatcggaccg gccgcgttag agccaacaat
     2041 gccatcctgt ccctgggggt aacgggacaa atctcggtct tcgcgagcgc cggcacgact
     2101 cacttcgtcc tcgatgtaaa cggctacttc gactagccag agaaaccctg ccctcggtgg
     2161 cgccggcgtt cagccgattc gcttcagaag gagtactccc gccgacgcaa gcagcatggc
     2221 aaggagcgcc agggcgggga aggagagagc gggaatcgca ccaggctgcc cgtcggcgct
     2281 cgagagatcc gggctcaacc gagccacgaa ggcatcgcta aagatggctc ggcgcgtctc
     2341 ttgcgcaccg ccagccgtcc ccggaaaatc gaatgagctt gtgtagccac caagataaac
     2401 gtctctggtg agggggtgga tcacagctgc ccgaaaagta tccgtgctac ttccacccaa
     2461 atacgtggcc tggagaagcc tggtaaggcc gggatcgagc tttgctgcga aggcgtcgcc
     2521 cgacggaccg ccgaagccag gctgagcgcc gcccgcagtg ccggggaagt ccctcgaaga
     2581 tgtaaatccg gctacgagga tgtcgccgct tccggggaag atcgacacgt cgataccgcc
     2641 gtcagactcg gtgccgccga ggtaggtcgc ttgcaggaga gctgtgaggg acgggttgag
     2701 cttggcgaca aaaccgtcgg aaagcccgct gcgaatcgcc ggctgcgcgc cgcccgcggt
     2761 tccaggcaaa tctgatgatc ctgtgatgcc cgccaagaga atctcgccgc ttgtcggaag
     2821 aatcgcgagg tcctgagcat cgtctatgcc gcttcccccg aaataggtcg cctgaaggag
     2881 cgtcgtgagc gcagtattga agcgcgccac gaacgagtcg cgacctcgtt ggatctcctg
     2941 tgcgccgccc gaagtttgtg ggaaatccgt agactccgaa atcccggtca gaaagagttc
     3001 actacctgaa ggatggatcg ccactgcggt ggtggcgtcg agagcgctgc cgcccacata
     3061 ggtggcctga agcagcgccg taagcgttgg gttaaaccga gcgacgaagc cgtctgcatc
     3121 accacccgcg taagctggct gtgcaccacc cgcggtgccc ggcagattcg ttgaggatgt
     3181 cgtacccgcc aggaaaatct ctccgctcgc agaatgtaca gtgagcgcga tgagattctc
     3241 agtttgatcg ccgccaaaat aggtcgcctg gagcagagcg gtgagcgtgg gactcaacct
     3301 cgctacgaag ccatcgtcct ctcctgccaa aaccgactgg gctccacccc cggtgccggg
     3361 gaaatctgtc gagttcgtca tgcccgccac caggacttcg ccactgcccg gagcgatggc
     3421 cagagagtcg gcttgttgga aaccgcttcc tcccacatat gtcgcctgga tcagccgtgt
     3481 gaggctggag ttcaaccgtg cgacgaaggc gccgctcccg gccggctgcg ctccccctgc
     3541 cgtgcccggg aagtcgttgc cggtactacc cgcgaccaga agctctccgc tcgccggatg
     3601 cacggcgatg tcgtgaaccg aggcaccgct tgcacctccc agatacgtgg actgcagcag
     3661 cgggtcgatc accaccggca aagcgagatc gtgagcgccc aggctgaagc cgtactccac
     3721 gcccttaacg cggtacgcga ccgcaacggg cgcgcgcgct tcgtccttct cctgataggc
     3781 gactggggcg ctgaatgtga cttcgccgag tcccgttagg gcggagagag tcccgctctt
     3841 gccgatcttg aggtcttgtg ccccatccac gctcagccgg atccggtcgg ctggagtgcc
     3901 gggcaagagc acaaaaaact tctctaccga gcctacgcgt gcgcgaaccg agacctcgat
     3961 accgggccat acgtctccaa gctcgaccga atcatgggtc gcaagccccg tccgccattt
     4021 cttagaatcg gtaccaagga agaagctgac cttcgccgct gcctgcgcgc cagcccgcgg
     4081 cctcggtgat ccatccagga agctctccgt cagggcccat ccgggccgga gttgccgttc
     4141 gcgcgtcgca gccgaaaaat tcgcctgttt ctctttcgct ccaggaaagg catacacgat
     4201 ctgtccggcg cgcgtcacga agaccgtgcc gccgagcatg gacgccgaaa atgccaccgc
     4261 cgggtcggcc tgacccgcat tggcaacgaa cggaactcgc aaccccgcta tcgaggcctg
     4321 cggaaggcgt tcgtcgggcg ggacgcctcc ggccaggcac agactctctg gcaggagcgc
     4381 taccgaggcg atcagaagaa cccgcggcgg tgatcggcgc ttcgacggag gagccaagca
     4441 actcagaacg gatatgtgca cgaatgcgcc aatctagcac gaagatcagg aaagcacgga
     4501 tacgcccttc tgtcggaccg ccagcctctc cgatgcaggc ccggacgggc aactcaacta
     4561 atgaacaaca tgttgagaga acacacttag cgcagaacgc tcgcgaggaa ctccttcgcc
     4621 acgtcctccg gacggcgctt ttccttatcg accgcggcgt tcaactttcg catcgtcgcc
     4681 gcgtcgatct tcccggcaaa cgactcgaga acttcgcgca cctcgggcct cttccacacg
     4741 gcgtcgcgga cgacgaacgc cgcctcgtag ggcggaaagt aatgccggtc gtcttcgagc
     4801 acgacgccgc cgatcgcgtc gatgagaccg tcggtcgagt tgcccgcgac gaggtcgacc
     4861 ttcccgctct caagcgccgg atagagcaga ccgaggtcca tctgcacggg gcgatcccgg
     4921 aagtcgaggc cgtaggtttt cgcgagaccg ggaaatccgt cctcgcgttc gagaaactcg
     4981 tagccgaaac cgggccggat ctccgggtgt gcggcgaggt cggagatctt ccggatgccg
     5041 agccgccgcg cgtcgtctcc tcggacgacg agcgcgaacg tgttctcgaa ccccagaggc
     5101 ggagaccaga cgagcttcca gcgccgatag gcgtcggcca ccccggcgcg gacggccgcg
     5161 gggtcggaga ccggccgccg tttcaggatc gcggtgaatg cggtgcccgt gtactcggga
     5221 tagaggtcga gctctcccgc cgtgatcgcc tggtggcaga cgaaggtgcc cccgaggttc
     5281 aaccgccggt cgacccgaat cccgcgggct tcgagtccct gcgccgcgat ctcgcccagg
     5341 atcacctgct cggaaaagtt cttcgagccg atgcggagcg cgcgatctcc gtcgcggcgg
     5401 caactggcga agaccgccag gaaagcggcg aagaggacga tccgcttcat cggccgaggc
     5461 gtttctccac gaaaccgaga ccagcgtccg ccgcgagggc gaggagcgcg gctggcagcg
     5521 cgccggcgag gatcaggcga tggtccacga tcgcgacgcc gcggtagatc aggacgccga
     5581 gccctccggc cccgatcgcg gcggcgatgg tcgcgagccc aattgagacg accgtcgcaa
     5641 tgcgcactcc ggc
//
LOCUS       DATIFQ010000190         3330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1595359, whole genome shotgun sequence.
ACCESSION   DATIFQ010000190 DATIFQ010000000
VERSION     DATIFQ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595359"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VLE22_08745"
     CDS             complement(<1..889)
                     /locus_tag="VLE22_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14535.1"
                     /translation="MNLASRFLPLLLLCIALACSSSAQNTGLGRIDFPTSGPPQAQTH
                     FVRGMLFLHSFEYDDAREEFRMAQKLAPGFAMAYWGEAMTFNEPLWFFQDRDSAQAAL
                     KRFAPTRAERQAKTPTDREKAYLQAVEVLFGEGEKEDRDFAYAEEMRNLHEKYPEDLD
                     AASFYALALLGTCHRGRDFRVYMKAATVVEEVFLKNSEHPGALHYLIHSYDDPIHAPL
                     GLRAARIYARVAPQAAHALHMPSHIFVAMGMWGEVVASNEDSWAASETRVKRKNLPPE
                     DRGYHSLWWLQYGYLQQGRF"
     gene            complement(886..2088)
                     /locus_tag="VLE22_08750"
     CDS             complement(886..2088)
                     /locus_tag="VLE22_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSB14536.1"
                     /translation="MSRDDTASCGFMSRRSLLNAMTAAGLAAGRIWAIEEDEYLVPFA
                     DYTSEFRVEAQASDPRVKCFDLRRMSSGATPNEEFFAFHQTGTPKVDAASWRLPVGGL
                     VERPKAFSLTDLERRPDRQDVAATIECSGNSGHPQLMNGLVSNGVWTGVTLASILREC
                     GVKAEAREVVFLGADSEKEEKWQAGNAEFVSPHGRSIFIQDAIAPGPMLAFRMNGAPL
                     PAEHGFPLRLIMPGWYGMASVKWLTRIEVIGRRYEGRHMARNYHSLRSVESPGDTIWL
                     DTSISKNNLKSVVARVTRRRVGNRFLHSIAGAAWGGESRIENVEVRVDDGAWRDARID
                     FRSGPFGWLLWSCEWPDAGTGKHTLVSRAINARNEIQPTRDEWRTRIASNREDNSQWP
                     RIIVIAGV"
     gene            complement(2202..>3330)
                     /locus_tag="VLE22_08755"
     CDS             complement(2202..>3330)
                     /locus_tag="VLE22_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSB14537.1"
                     /translation="WPAMGKLAVITASFDRSADSFEVSPDGRTVYVLAEEHGHDKIFA
                     VPAAGGEAKPVLDVKLGCYSGLAVPEKAEKTILFAAWQSAVNPPEIVRIDPGAAQHTL
                     LSDFTVETAAAVDWQPARHFWFTSKRGKQIHNMIVLPPAFDENKKYPLLVFMHGGPHN
                     MSKDQFILRWNYHLLAQPGYVVLTTNYSGSTGFGEKFAQEIQGDPLKGPGDEINEAAD
                     EAIRRFPFIDATRQAAAGASYGGHLANWLQATTTRYKCLVSHAGLINLESQWGTSDTI
                     YHRERGNGGPVWEQGPVWREQNPIRFAANFRTPILLTVGEKDYRVPINQTIENWSVLK
                     RLKIPSRLIVFPDENHWILKGENSRFFWKELHEWLKQYVGD"
BASE COUNT          620 a         1058 c          950 g          702 t
ORIGIN      
        1 cgaatctgcc ttgctgaagg tagccgtatt gaagccacca gagcgaatgg tatccccggt
       61 cctcgggagg caggttcttt cgcttcacgc gagtctcgga ggcggcccat gagtcttcgt
      121 tcgacgcaac tacctcaccc cacattccca tcgccacgaa aatgtgggag ggcatgtgca
      181 gcgcatgggc ggcttgcggc gcgactctgg cataaatacg ggccgccctc aagcccaagg
      241 gcgcgtgaat cggatcgtca tacgaatgga tcaggtaatg cagtgcgccg gggtgctccg
      301 aattcttgag aaacacctcc tcaacaacgg tggcggcctt catgtaaacg cggaaatccc
      361 ggccccgatg gcaggtaccc aacagggcaa gagcatagaa ggaggccgca tccagatcct
      421 ctggatattt ctcatggaga ttgcgcatct cctcggcgta cgcaaaatca cgatcctcct
      481 tctcgccttc gccgaagagc acttccacag cctgaaggta agctttttcg cgatccgtgg
      541 gagtcttcgc ctgcctttct gcgcgagtcg gagcaaaacg cttcagagcg gcttgcgctg
      601 agtcgcggtc ctggaagaac caaagcggtt cattgaaggt catggcctct ccccaatagg
      661 ccatggcgaa gccgggggcg agcttctgag ccatccggaa ctcttctctg gcgtcgtcgt
      721 actcgaagct gtggagaaac agcatgccgc gtacaaagtg cgtctgggcc tgcggaggtc
      781 ccgaagtcgg aaagtcgatg cgccccagtc ccgtgttctg ggcgcttgaa ctgcacgcca
      841 gggcgatgca cagtaacaac agaggtagga accggcttgc cagattcaca ctcctgcgat
      901 cacgatgatc cgcggccact gagagttgtc ttcccggttg cttgctattc ttgtgcgcca
      961 ttcgtcacgg gtcggttgaa tctcgttcct ggcattgatg gcgcgagaca cgagagtatg
     1021 ctttccagtc cctgcatccg gccactcgca cgaccaaaga agccagccga acgggccgga
     1081 ccggaaatcg atccttgcat cccgccacgc tccgtcgtcg actcgtactt ccacattctc
     1141 gattcttgat tctcctcccc aggctgctcc tgcaatgctg tgcaggaagc ggtttccaac
     1201 ccgccggcgc gtcacccgcg cgaccaccga cttcaagttg tttttcgaga tggaggtgtc
     1261 gagccagatt gtgtcacccg gcgactcgac agagcgcagg ctgtggtagt tcctcgccat
     1321 atggcgcccc tcgtagcggc ggccgattac ttcaatcctt gtgagccact tcacgctggc
     1381 cattccgtac catccgggca tgatgagccg gagcggaaag ccgtgttcgg ccgggagagg
     1441 cgcgccgttc attctgaaag caagcatcgg ccccggggcg attgcgtcct ggataaagat
     1501 gctccggcca tgtggtgaga caaactctgc attgccggcc tgccacttct cttctttttc
     1561 cgagtccgcg ccgaggaaca cgacctcgcg ggcttcagcc ttcactccgc attcgcgcaa
     1621 gatcgaagcg agtgtcacgc cggtccacac cccgttgctg actaagccat tcatcagttg
     1681 cgggtgtccg gagttgcccg agcactcgat cgtcgccgct acgtcctgcc tgtcgggtcg
     1741 ccgctccaga tctgtcaggg agaacgcctt tggtcgctcg acgagccccc cgactggcag
     1801 cctccaactc gctgcgtcca ctttcggcgt tccggtttga tggaaggcaa aaaattcctc
     1861 attgggcgtc gccccggagc tcatccgcct taaatcgaaa catttgactc gtgggtcgct
     1921 cgcctgggcc tctacgcgga attccgaggt gtaatcagcg aacgggacaa ggtattcgtc
     1981 ctcctcgatg gcccagattc ggccggcggc aaggccggcg gcggtcatcg cgttcaatag
     2041 actccggcgg ctcatgaaac cgcacgacgc cgtatcatcg cgagacataa caggttttcg
     2101 cacgccccag aatgaagtaa taaagctatt atcgtataat ctcgccggcc ggttcggttt
     2161 cgcgggctat tccgagcctt cgccggagcg tacttctcgt tctaatcacc gacatactgc
     2221 ttcagccact cgtggagttc cttccagaag aaacggctgt tttcgccctt caaaatccag
     2281 tggttctcgt ccgggaacac aatcagacga ctggggatct tcaggcgctt cagcacgctc
     2341 cagttttcaa tcgtctggtt gatcggtaca cggtaatcct tctcgccaac cgtcagcagg
     2401 atcggcgtgc ggaagttcgc cgcaaatcgg atcgggttct gctcgcgcca caccggcccc
     2461 tgctcccata ccggtccgcc gttgccgcgt tcccgatgat agatcgtgtc acttgtcccc
     2521 cattgcgact cgaggttgat cagccccgcg tggctcacca ggcacttgta ccgcgtggtc
     2581 gttgcttgca gccagtttgc gagatgcccg ccgtaactgg cgcctgccgc cgcctgccgc
     2641 gtggcgtcga tgaacggaaa ccgccggatc gcctcgtcgg ccgcctcgtt gatctcatcg
     2701 cccggaccct tcagcgggtc gccctgaatc tcctgcgcga acttctcccc gaatccggtt
     2761 gagccgctgt agttcgtcgt cagcacaacg tagccgggct gcgcgagcag atgataattc
     2821 caccgcaaaa tgaactggtc tttcgacatg ttgtgcggcc cgccgtgcat gaagacgagc
     2881 agcgggtact tcttgttctc atcgaacgcc ggcggcagca cgatcatgtt gtggatctgc
     2941 tttccgcgtt tgctggtgaa ccagaaatgg cgcgcgggct gccagtccac agcggccgcg
     3001 gtctcgacgg tgaagtcgct cagcagcgtg tgctgtgccg cgcccgggtc gatccgcacg
     3061 atctccggcg gattcaccgc gctctgccat gccgcgaaca gaatcgtctt ctcagccttc
     3121 tccggaacgg ccagcccgga gtagcagccg agtttcacgt cgagcaccgg cttcgcttca
     3181 ccgcctgccg cggggacggc gaagattttg tcgtgcccgt gctcctcggc gaggacataa
     3241 acggtccggc cgtccggcga aacctcgaaa gagtcggcgg agcggtcaaa actcgctgtg
     3301 atcacggcga gtttgcccat cgccggccac
//
LOCUS       DATIFQ010000191        16693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_63657, whole genome shotgun sequence.
ACCESSION   DATIFQ010000191 DATIFQ010000000
VERSION     DATIFQ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16693
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63657"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..203)
                     /locus_tag="VLE22_08760"
     CDS             complement(60..203)
                     /locus_tag="VLE22_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14538.1"
                     /translation="MFQRLKGEMDQWIEAQTQVRKFAGAKGQTTLSPKAIERHWSLGY
                     IGK"
     gene            955..1344
                     /locus_tag="VLE22_08765"
     CDS             955..1344
                     /locus_tag="VLE22_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14539.1"
                     /translation="MTKLVWTLWIVMLALVVSGEVLPGTSPPMRWVSSTGISDKVLHY
                     GAYTLLAGIPTLGFPGVVGVLCAMAMIPLGVVLEFIQKAVPGRGFELGDITANTLGVF
                     TGVAAGWAGRKVRSIVAMRLPEGRHGP"
     gene            complement(1532..3376)
                     /locus_tag="VLE22_08770"
     CDS             complement(1532..3376)
                     /locus_tag="VLE22_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14540.1"
                     /translation="MKATASSVPPLGLVDLGSPDLTGKYRVPGVRAAWSRWHGSRQLA
                     VSQSPSRLLWIEGHPDRMPAEGEELTRWLEGRSGSFRGFEIHSSSGRPMRFRAFVDPL
                     GTRPVYYLADQDRVLFADKLATLALNSTCAAAVQWPAVLESLTIGVLYSADTSLENAR
                     QMVPGELIEAGAGGEWSTRRFALPADAAIEPDRVHRDPAGTLRRALAKAVAETWRCPD
                     SILLLSGGLDSRFALAVAGPGRSVLSVTPRENRESRVARLVAESCQARFYHFLCHADH
                     YFHVLRRSYLITGCLWEPWEAHHLGLASHWRRQGFRGLTHAYHCDSLLKGNRIFPWQK
                     RVPSVTPLHSIMGDRAICYAQCRSFRWHPQSSELVYGTLSATGQDVARQRLRQLASEL
                     EPVLEDGLELTVERQFHQRVAAGHSYPLLLSWMEEQDVYSPIYHPAPWSWAKTSRPTD
                     RFRGRAFRQALLEMDHPASLVIDANTGKLISAARRDWGGLIRNLPGLHVLREWRDRMR
                     YSRLLASPEPEAEEGSWPLLAPLFRSPEGLRIMEEGHACLPPEFFNMDNVRRAVGAFA
                     GGDDRYLETVLDLSAAGRWARFCAEGPATDERDLCWRAAFQSPAHGGP"
     gene            complement(3519..3605)
                     /locus_tag="VLE22_08775"
     tRNA            complement(3519..3605)
                     /locus_tag="VLE22_08775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3569..3571),aa:Leu,seq:tag)
     gene            complement(3781..4713)
                     /locus_tag="VLE22_08780"
     CDS             complement(3781..4713)
                     /locus_tag="VLE22_08780"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB14541.1"
                     /translation="MNTPWGDIDVSDAHVHFFSHRFFTLLAAQTNEHATVETIGARLG
                     WEMPPQDNAALAGRWAAELDRHGVLRAALIASLPGDEDSVASAVAAFPERFHGYFLVN
                     PCEPEAPARVERALASGQLRCVCLFPAMHRYSIQDERVEAIFEKAAARPGTLVFVHCG
                     VLTVGVRRRLGLKSAYDLRFSNPVDLHTVALRYPKLNFVIPHFGAGYLREALMVCDLC
                     PNVYLDTSSSNSWIRYISPEIRLADVFRRALEVAGKQRLLFGTDSSYFPRGWHAAVFH
                     DQVRALSEAGVEEEVAREIFGGNLDRLLGFANRT"
     gene            complement(4723..5403)
                     /locus_tag="VLE22_08785"
     CDS             complement(4723..5403)
                     /locus_tag="VLE22_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB14542.1"
                     /translation="MPFQILLVDDHKIMRDGIKAILEHTGGEFEVVGEAENGTDAVQV
                     ARKTRPDVILMDIGLPGVNGIEATAEILRYLPDTKVIILSMYDDEHSVISAIRSGARA
                     FVLKKASDSDLLDALRTVAKGGSYLSPQVSDRLLSRIQRGEPDAKATPSVLEGLTPRE
                     LQVMRLVAEGRTSKEIAMLLDLGLQTVRSYRKSMMKKLGVNNVAGLTQTALAAGLTRF
                     PKPEQGER"
     gene            complement(5440..6141)
                     /locus_tag="VLE22_08790"
     CDS             complement(5440..6141)
                     /locus_tag="VLE22_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSB14543.1"
                     /translation="MAVYEQSLARLVVTLMNAQEQEWARVSRVLHDEVSQVMSAVGLQ
                     LDVLRMDLQDRIPEIPARTAEIQKLLERAISLVRELSYELNPAIVEKAGLQFALERLI
                     GRYRKRYSGTLRLMVDLAELLPPDVASALYKIAHQAIENAVHHSEASLIEVMIKPVSR
                     TTVLEVRDQGIGFRIEELKGKVPGLGLLMMQYYASQADIDFSVTSEPGKGTIVRAVYP
                     SSRSRREAAGTAKKP"
     gene            complement(6266..7234)
                     /locus_tag="VLE22_08795"
     CDS             complement(6266..7234)
                     /locus_tag="VLE22_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSB14544.1"
                     /translation="MATRTKFELKMGMATREAFGKALVALGRENKDVVAVDADLSKST
                     MTHFFAKEFPDRFFSCGIAESNMVGIAAGLAAAGKIPFAASFACFVVNKGFEQMRVVV
                     AYPNFNVKIVGTHSGISIGEDGPSQMSVEDLSLACSLPGFTVLSPADEVAMAALVRQA
                     AAHFGPVYIRAGRPKVPVVYQTGQRFEIGKAVELINGSDLTIIATGLMVAEAIRAQEH
                     LETEGISARVVDIHTVKPLDVEAIRRAASETGAIVVAEEHLVDSGLGVRVAQAVAETQ
                     PCPMEFVGIQNTYAESGPPDQLLDKYGLVARDVIAAAYRVLRRKQH"
     gene            complement(7234..8055)
                     /locus_tag="VLE22_08800"
     CDS             complement(7234..8055)
                     /locus_tag="VLE22_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSB14545.1"
                     /translation="MERKKNLEELQEMCKRVRRHIIEMTGAAKSGHPGGSLSAVEILV
                     TLFFDVMKHDPANPQWPDRDRFILSKGHGCPVLYAVMAECGYTPVDQLKTLRKLGSPY
                     QGHPDKRFIPALEASTGSLGQGLSLALGMGCAARLDGKPWRTYVVLGDGEIQEGQVWE
                     AAMFGAYHKIDNVVAIVDYNRIQLDGWVKDIMEVEPLVPKWQSFGWHVIELDGHNIPA
                     LQAAFTEAEAIKGKPVAIIAHTVKGKGVSFMENNPKFHGVAPTPDEVQVALKELQ"
     gene            8103..8519
                     /locus_tag="VLE22_08805"
     CDS             8103..8519
                     /locus_tag="VLE22_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14546.1"
                     /translation="MAVEIRKIDYYYAIVPDKPGEGARILSALHQAGINLIGFSGFPA
                     GARKAQLDFLPEDSAAFAKAARNAGLKLSKKKAGLLIQGEDQPGAGAEIFGKLAAAGV
                     NVTSLQAVSAGAGRFGGMLWVKATDMRKAVKALGAA"
     gene            complement(8532..9500)
                     /gene="hemB"
                     /locus_tag="VLE22_08810"
     CDS             complement(8532..9500)
                     /gene="hemB"
                     /locus_tag="VLE22_08810"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HSB14547.1"
                     /translation="MAFPVHRMRRLRATASLRDLVRETRLTPSQFILPLFACPGEGVR
                     REIGAMPGNYQMSVDEMVKECAEVRSLGIGGVILFGIPETKDETASGAYAEDGIVQRA
                     VRAIRRDVPGLLVLTDVCNCEYTSHGHCGKVVNDDVDNDATLEWLAASAVSHARAGAD
                     IVAPSDMMDGRVAAIRRALDASGFPGVPILAYAAKFASVFYGPFREAAESAPQFGDRR
                     SYQMDPANGREAMREIELDLEEGADMIMVKPAMPYLDLIRQARDRFPVPVAAYQVSGE
                     FSMIVAAARNGWLDRERAMMETLTSIRRAGADIILTYFAKDAARLL"
     gene            9557..9847
                     /locus_tag="VLE22_08815"
     CDS             9557..9847
                     /locus_tag="VLE22_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HSB14548.1"
                     /translation="MGRPKDSAAVARRYLVRGRVQGVGYRYFVERAAYELGVTGYARN
                     LEDGRVEVYAVGPPDRLSALAGFLWKGPRLSNVRGVEELDAPLQKYAGFHIE"
     gene            9807..10433
                     /locus_tag="VLE22_08820"
     CDS             9807..10433
                     /locus_tag="VLE22_08820"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HSB14549.1"
                     /translation="MRRSRNTPDFTSSNPGIVSAGCYNTLFPRTIRPMNNDQLKKLIR
                     EVPDFPKPGILFYDITTLLKDASGLKAVIDAFGKRYQGSSVDVVLGIEARGFIFAPAV
                     AYALGVGFVPVRKPKKLPAETVREEYQLEYGTDSLEIHKDAIQPGQRVLIVDDVLATG
                     GTAAAVTRLIEKLGGKVAGLGFVLELDFLHGRDKVAGYELFSLLHYEK"
     gene            10430..11407
                     /gene="ispE"
                     /locus_tag="VLE22_08825"
     CDS             10430..11407
                     /gene="ispE"
                     /locus_tag="VLE22_08825"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HSB14550.1"
                     /translation="MSTGLRHARVEALAKINLALKVLHKRTDGYHELRTVFQTISLGD
                     TLEIEFTPSRRTRIELVSRVDIPDNLVARAARSVMDAIRVTGTVRLRLVKRIPMGAGL
                     GGGSSDAAAVLLALPVLAGGRIPADQLIGLAAGLGSDVPFFLVGGTAAGIGRGTEVYP
                     LPDHPAFPGILVAPPIHVSTAEAYRGLDRKLTSVVPPNIMNNFQSFAWSGGLGEPGKG
                     TPASPGNDFEESVFRLHPRLKSLKARLSRIGAAPVMMTGSGSALFGLFRGRNDLERAL
                     PLFEEETVFRFSLVTRERYRRAWMRWLAAHIEGEIWPPQSRYSKTKTTG"
     gene            11362..12333
                     /locus_tag="VLE22_08830"
     CDS             11362..12333
                     /locus_tag="VLE22_08830"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HSB14551.1"
                     /translation="MAAAKPVLKNKDDRLKVFTGNANPSLAAELCACLGCTVGSALVK
                     PFSDGEMYVQIQENVRGADVFVVQPTCTPVEQHLFELLLMLDALKRASADRITAVLPY
                     YGYARQDRKDKPRVPISAKLIASLLERAGADRILALDLHAAQIQGFFDVPVDHLFATP
                     VMIDYFKSLGIGNLTVVSPDAGGVERARAFAKRLKAPLAIIDKRREQMNVAEVMHIIG
                     RVRGRNCLIVDDLIDTAGTLVNAAAAMLDHGALSVTACATHAVLSGPAVDRIEVSGLK
                     EVVLTNSIPLREDAKRCKRIRTLSVARLLAEAIRSIHEETSVSKLFV"
     gene            12397..13062
                     /locus_tag="VLE22_08835"
     CDS             12397..13062
                     /locus_tag="VLE22_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HSB14552.1"
                     /translation="MRKDITVSATVRDSRGKNEARRLRVTGMVPAVVYGAGQKPLSLS
                     VDPKQLLKILHSKTGHNTIFSLTTGDGPSPVMIVDWQFEPVKGTLLHVDLKRIDLSKR
                     LRVSVPVYTQGEPKGVKQQGGLLEIVNREMEIECLPDDIPEHFTVDVTELLIGQNIRA
                     ADVPIGESVKLLSHGDAVIAHVVTLRHEEEKPAEEAPAAEGAAPAEPEVIKKGKKEEE
                     GEE"
     gene            13092..13703
                     /gene="pth"
                     /locus_tag="VLE22_08840"
     CDS             13092..13703
                     /gene="pth"
                     /locus_tag="VLE22_08840"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSB14553.1"
                     /translation="MEADSRATPFLLVGLGNPGERYEQTPHNLGFLVIDRLAERHRIR
                     VSRKEARSLTGTGTVEGIPVVLAKPQTYMNLSGGAVGALLAKHGARAEDLVLVYDDLD
                     LPWTAVRIRPKGSPGGHHGVESVIRAVGTSEFPRVRLGIAGYKVGDGAEFVLSPFRRG
                     QKEELDELLDHAAQAVTSIISEGVGMAMTRFNRRARGKQTEEE"
     gene            13700..14134
                     /gene="rpsF"
                     /locus_tag="VLE22_08845"
     CDS             13700..14134
                     /gene="rpsF"
                     /locus_tag="VLE22_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSB14554.1"
                     /translation="MRIYEELFILKPDATEEEIDQVVEQLRQVITGGGGEVDKIDKWG
                     LRRLAYRVHKYNEGFYVLIQFRASPEVVRELERRLRVTDLAVKFLTVRIDEKLKRIEK
                     RKKQREKRAARKPAAPVAAPQPPAAEEIMAEAGAADAGTERE"
     gene            14134..14433
                     /gene="rpsR"
                     /locus_tag="VLE22_08850"
     CDS             14134..14433
                     /gene="rpsR"
                     /locus_tag="VLE22_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSB14555.1"
                     /translation="MAETKGRPVSASQRPTGGDKAIAVGRKQYFRRKKVCRFCVDKID
                     DIDYKDVKLLSGFVSERGKITPRRISGVCTPHQRRLSEAVKQARNIALLPFTTQV"
     gene            14551..14997
                     /gene="rplI"
                     /locus_tag="VLE22_08855"
     CDS             14551..14997
                     /gene="rplI"
                     /locus_tag="VLE22_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSB14556.1"
                     /translation="MEVILREDIEKIGHRGQVVRVADGYARNYLLPRRLAVQATDANK
                     TIVEQERQAHLRREAKEKSEAGDLAKLLAGVKITISQKAGEQEQLFGSVTSKDIAEAL
                     EKQNFTIDRRKIHLDEPIKQLGDYKVAIRLHHDVTAEVDVHVTKEE"
     gene            15010..15768
                     /locus_tag="VLE22_08860"
     CDS             15010..15768
                     /locus_tag="VLE22_08860"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSB14557.1"
                     /translation="MAFFDLTGHTAVVTGAATGIGEAVVRRLAAAGASVAAVDLDMEG
                     AARVAGDIAGGPGRLVFAVRADISSAESVNQAVAEVLDRTGRIDVLVNNAGIAGRAAP
                     LWEQTVEDWQRVIAVNMNGVFHCCRAVINHMRERHYGRIVNVASIAGKEGNPNMTGYS
                     STKAAVIAFTKSLAKEVATEGICVNSVAPAVVRTRILDQLTPAQIEYMTARIPMRRTG
                     TPDEIAAVIHFLASPDCSFVTGQCYDASGGRATY"
     gene            15746..>16693
                     /locus_tag="VLE22_08865"
     CDS             15746..>16693
                     /locus_tag="VLE22_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14558.1"
                     /translation="MAAALPTNVILSPNQYPSVPVYDLLVAAANGQAPFDHPFLNALF
                     ERFDSAVPDLVRFGMDDRTHDLIDLEPELVAIFRYKPTSLALPFLIKAVHRQPDDLSE
                     ELIDAFHRVGPLAVEPLLELYQRLGPEEGGEVSFTLAALRVRDQRIFDVLINRLSVDP
                     GDAAIQLGLYGDPAAKPALEKLLREFEANGDAPPSTISEIRCALQDLDEPRAEETAEP
                     YDIWSDYPEAALPLFEHLSEQERTKFLGSPVAEYRARAAESLGPELSATVKAHLFEMA
                     RTDPEAPVRGACWERLRDALPDTDVRAAMIERLSDETAPQ"
BASE COUNT         3238 a         5008 c         5354 g         3093 t
ORIGIN      
        1 ggtgggacaa cctgcgcgtg tatcgcgtcc tgaagtgacg attgctacag gccgggctac
       61 tacttcccga tgtaccccaa gctccagtgc cgttcgatcg ccttcggact cagagtagtc
      121 tggcccttgg cgccggcgaa cttgcgcacc tgcgtctgcg cttcgatcca ctggtccatc
      181 tcgcccttca gcctctgaaa cacgtcagga tgatgtgccg ctacgttcct cgtctccgcg
      241 cggtccgtcc ggcggaagga ggtgacctgc ctcgtgtcaa cttgctctgt gcggtgtttg
      301 tgctcacaag acgcccttga tgctgcgcct gccggttccc ggtgtcaatc ggaatcgtcg
      361 acactcatcg acgtgagacc ttgtcaccgc gggagcgggg tggtaacctt cgatggacca
      421 gtggagattc caatggagac gaccgaaggt gccgagtgtc ctctctgtct cgaacagatg
      481 caaatcgagg agcgggatgg aaggacgtgg ctggtttgcc ctaacggttg tccgacggaa
      541 actgagctcc tcctccgaaa acctcctgaa tcggaatcag agaatggtat caccgagttt
      601 tggtcgcggg cgggcagatc gtcggcgcga taatgcgcct gaagttcatc acccttccgg
      661 gtgaccggcg tttgattcag aaactgtcta tccagcagcc cacgttgttc gaagtagacc
      721 tctcctcggc ggtttgcatt gaacactgac tgcggaagtc ggctccatga ggctcggatg
      781 ggattattcc ggacaacagt tgagccagaa atctgcgagt cgatccgcag ccggctcatg
      841 aaaccagact gcgagcacat ctgccaaaat cgaagtctga gttgatcgtt ctccaacgaa
      901 gtgaattggg ggctgacgaa ttgcacgttc ggagcgaagg gcgctgacgc cgtgatgaca
      961 aaattggtgt ggactctgtg gatcgtaatg ttggctttgg tggtttcggg tgaagtgctg
     1021 ccggggacat cgccgcctat gcggtgggtt agttcaactg gaatcagcga caaagtgctc
     1081 cactacgggg catacacgct actggctggg ataccgactc tggggttccc cggggtggtt
     1141 ggcgtactgt gtgcgatggc aatgattccg ctcggcgttg tgcttgagtt tatccagaag
     1201 gcagtgccgg gccgcggttt cgaactgggt gacatcactg ccaacacgtt aggggttttc
     1261 acgggtgtcg cggcggggtg ggcgggacgg aaggttcggt ccatcgtagc gatgcgactg
     1321 ccggagggca gacacgggcc gtaacgatgg tacggtcgtt gccaacaggc ttcaaggacg
     1381 attccggtgc aaaccagggg cgcggattcc aatcatcggc gtcgagattc gtctcgacgc
     1441 cgccgctcga agaaagatct gccctcggca ccagtgtctc tcgaggccgc gaacaggcgt
     1501 gcttgcgcca ccaaaacacc aagtccgatc ctcacggccc tccgtgagcc ggggattgga
     1561 aggcagcccg ccagcacagg tcacgttcgt cagtcgccgg cccttccgcg cagaaacgtg
     1621 cccagcgccc ggcggcgctg aggtccaaca cggtctccag gtaacgatcg tcaccgccgg
     1681 caaacgcgcc aacggcccgg cgcacgttgt ccatgttgaa gaactccggg ggtagacagg
     1741 cgtggccttc ttccatgatg cgcagtcctt ccggggatcg gaacagcggc gccaggaggg
     1801 gccaggatcc ctcctctgct tctggttcgg gggatgccag gagccgggag taccgcatcc
     1861 gatctcgcca ctcccgcaag acgtgcaggc cgggcagatt gcggatcaag ccgccccagt
     1921 ctctgcgagc ggcgctgatc agcttaccgg tgttcgcgtc gatgacaagg gaagccgggt
     1981 ggtccatctc caagagggcc tgccggaacg cccggcctcg aaaccggtca gtgggccggc
     2041 tggtcttggc ccaggaccaa ggggcggggt ggtagatcgg tgagtaaacg tcctgctcct
     2101 ccatccacga caagagaagg gggtaactgt ggcctgcggc cacccgctga tggaactggc
     2161 gttccacggt cagttccagc ccgtcctcca atacaggctc cagctcggaa gccagttgcc
     2221 ggagccgctg ccgcgccaca tcctgccctg ttgcgctgag tgttccgtag accagttccg
     2281 aactctgggg atgccagcga aacgaacggc actgcgcata gcagatcgcg cgatccccca
     2341 tgatcgagtg aagcggcgtc acgctgggga cgcgcttctg ccaggggaaa atcctgttcc
     2401 ctttcagcag cgagtcgcag tggtaggcgt gagtcaatcc ccggaatccc tgtcgccgcc
     2461 agtgcgatgc caggcccagg tggtgggcct cccaaggctc ccacaagcag cctgtgatca
     2521 ggtaacttct tcgcagcacg tggaaatagt gatccgcgtg gcataggaaa tggtagaacc
     2581 tcgcctggca gctttcagca acgagccgcg cgacacggct ttcccggttc tcgcgagggg
     2641 tgacgctgag cacgctgcga cccggtccgg cgacggcgag tgcgaacctg gaatcgagcc
     2701 cgccactgag cagaagaatg gaatcagggc atctccaagt ttcagccact gccttcgcca
     2761 gagcccgccg aagagttccc gccgggtctc gatgcacgcg atccggttcg atcgcggcgt
     2821 cggccgggag tgcgaaccgg cgggtactcc actcgccccc ggcgccggcc tcgatcaatt
     2881 cgccgggcac catctgcctc gcgttctcca ggctcgtgtc cgccgaatag agaacgccga
     2941 tggtgagact ctcgagtacc gctggccact gcacggccgc ggcgcaggtg gagttcagcg
     3001 ccagcgtcgc cagtttgtcg gcgaacagaa cgcggtcctg gtccgccagg taataaaccg
     3061 ggcgagttcc gagcgggtca acgaaggctc ggaaccgcat cggacggccg gaggaggagt
     3121 gaatctcaaa tccgcgaaag ctgcccgacc ggccttcgag ccatcgagtc agttcttctc
     3181 cctcagccgg catgcgatcc gggtgcccct cgatccacag cagacgggac ggactctgag
     3241 aaaccgccaa ttgccggctg ccgtgccagc gggaccaggc ggcccggaca cccggcaccc
     3301 ggtacttgcc agtcaagtca ggagaaccca ggtcaaccag cccaagcggc ggaaccgagg
     3361 atgccgttgc tttcatccac agctccacgc agaatcctga catctcttaa ttatggctcc
     3421 gagactccac tcgggccggg gtttccgtca ccactgacat cgtgaacact caggatccta
     3481 actcgccgcg gccacgacac gtaagctgtt gaattgattg gtgcggacgg ggggagttga
     3541 acccccacgc cctttcgggc actggaacct aaatccagcg cgtctgccgg ttccgccacg
     3601 tccgcgtttg gtgcgcttcg acgccatcat agcatcggcg ggcgcgaacc gcaggtcagt
     3661 ccgcagcgga ggcggcgaat accgcagtcg tctccgagcg gcacagtttc cgctggtgaa
     3721 gcccaagcag gcgcgggccg cgcagaccgc agccgccggc agagaaacct ccggttcttc
     3781 ttaggttcgg ttcgcgaagc ccagaagccg gtcaagattc ccgccgaaaa tctcgcgggc
     3841 gacctcttcc tcgacgccgg cctcgctcag cgctcggact tggtcatgga agaccgcagc
     3901 gtgccagcct cgcgggaagt atgaggaatc tgttccgaac agaagacgtt gtttccctgc
     3961 aacctccaaa gcccggcgaa atacgtccgc cagacggatc tccggagaaa tgtagcggat
     4021 ccaactgttg ctgctggaag tgtccaggta gacattcgga cagaggtcgc agaccatgag
     4081 cgcttcccgg agatagccgg ccccgaagtg agggatgacg aagttcaact tcgggtagcg
     4141 aagggcgacg gtgtggagat cgacgggatt cgagaaccgg aggtcgtagg cggacttcaa
     4201 tccaagcctc cggcgcacgc ccaccgtcag cacgccgcag tggacaaaca ccagcgtgcc
     4261 cggacgtgcc gctgcctttt cgaaaatcgc ctcgacccgc tcatcctgta tggagtagcg
     4321 gtgcatggcc ggaaagaggc agacgcatcg cagttggccg ctcgcgagtg ctcgctcgac
     4381 ccgcgccgga gcctccggct cgcaagggtt cacaaggaaa tatccgtgga agcgctccgg
     4441 gaaagcggcc acggcggagg caacagagtc ttcgtcgccg ggcaagctgg cgatcagcgc
     4501 cgcacgcaag acgccgtgac gatcgagttc cgcggcccaa cgcccggcaa gggcggcgtt
     4561 gtcctgtggc ggcatttccc atcctaacct cgcaccgatc gtctcaacgg tggcgtgctc
     4621 gttcgtttga gccgccagaa gcgtgaagaa gcgatgcgaa aagaagtgga cgtgagcgtc
     4681 ggaaacgtcg atgtcgcccc aaggcgtgtt cataccacag ggttagcgct cgccttgctc
     4741 cggtttcgga aaccgcgtca agccggcggc gagcgccgtt tgggtcagac cagcaacgtt
     4801 gttgacgccc agcttcttca tcatgctttt ccgatagctg cggacagttt gcaggccaag
     4861 gtcgaggagc atcgcaattt ccttgctggt tctgccctcg gcgacgagcc tcatgacctg
     4921 gagctctcgg ggcgtcaggc cttcgagtac cgatggtgtg gctttcgcat ccggctcgcc
     4981 gcgctggatg cggctcagca gccggtctga aacctgcggg ctcagatagg agcctccctt
     5041 ggcaacagtc cgcaaagcgt ctagcagatc gctgtcggac gccttcttca ggacaaacgc
     5101 gcgcgcgccc gagcgaatcg cgctaatcac tgaatgttcg tcgtcgtaca tcgacagaat
     5161 gatgactttt gtgtccggca agtagcgaag gatttccgcg gtggcctcaa tgccgttaac
     5221 ccctggaagg ccgatgtcca taagaatgac gtccggacgg gttttcctgg cgacctggac
     5281 agcgtcggtt ccgttttcgg cctctccgac gacttcgaat tcaccgccag tatgctcgag
     5341 gatcgccttg atcccatcgc gcatgatctt gtggtcgtcg acgagcagga tctggaaggg
     5401 catgtcactt ctccgccggc aaacaccggc cggcgagcct cacggtttct tggccgtccc
     5461 cgcggcctcc cgtcgcgaac gtgacgacgg atacactgct ctcacaatcg taccctttcc
     5521 aggctcgctc gtgacggaga aatcgatgtc cgcctggctg gcatagtact gcatcatgag
     5581 cagaccgagg cccggaactt tgcctttgag ttcttcaatg cggaagccga taccctggtc
     5641 ccgcacttcg agtacggttg ttctggaaac gggtttgatc atcacctcaa tcagcgaggc
     5701 ctcggaatga tggaccgcat tctcaattgc ctggtgggcg atcttataga gggcgctggc
     5761 gacgtcgggc gggaggagct ccgcgaggtc caccatcagc cgcagtgtgc cggagtatct
     5821 cttccggtag cggccgatca gccgctcgag agcgaactgg aggccggctt tctcaacgat
     5881 ggccggattc agttcgtagc ttagttcccg tacaagggaa atggcgcgct ccagcagttt
     5941 ctggatctcg gcggtgcggg cggggatttc cggaatccgg tcctgtaagt ccatgcgaag
     6001 cacgtcgagc tggagaccga cggcgctcat aacctggctc acttcgtcgt gcaaaacacg
     6061 ggagacgcgc gcccattcct gttcctgggc gttcatcaga gtgaccacca accgggctag
     6121 ggactgttcg tataccgcca tggaatcctg atgttaattc cgctaatgat gactgatttt
     6181 catcctcgcc gcaaggtaaa ttgacacggt cggtggaccg ggacagcgga cttttacccc
     6241 tgaacgcagt gtcgctcccg ccgcgtcaat gctgcttacg ccggagcacg cggtaggcgg
     6301 cggcaatcac atcgcgtgcg acgaggccgt atttgtcaag cagttgatcc ggcggcccgg
     6361 attcggcgta tgtgttttgg atgccgacga attccatcgg acacggttgc gtctctgcga
     6421 cggcctgagc gacccgcacg ccgagtccgc tatcaacgag gtgctcctca gcgacaacga
     6481 tcgctcccgt ctcggaggcg gcccggcgta tggcttccac gtcgagcggc ttgacggtgt
     6541 ggatgtcgac cacgcgcgcc gagatcccct ctgtctccag atgttcctgg gctcggatgg
     6601 cctcggccac catcaacccg gtggcgatga tggtcaagtc ggatccgtta ataagctcga
     6661 cggcctttcc gatttcgaac cgctggccgg tctggtagac gacaggtacc ttaggccgtc
     6721 ccgcacggat gtatacgggg ccgaaatggg ccgcggcctg ccggacgagt gcggccatgg
     6781 cgacctcgtc cgccggggaa agaacggtga accccggcag cgagcaggct agactcaggt
     6841 cctcgacgct catttgggag gggccgtcct caccgatcga gatgccgctg tgcgtgccga
     6901 caatctttac gttgaagttg gggtacgcta caacgacccg catctgctcg aatcctttgt
     6961 tcaccacgaa acaggcgaaa ctcgccgcga acgggatctt gcccgccgcc gccagcccgg
     7021 cggcgattcc gaccatgttc gattcggcga tgccgcaaga gaagaatcgg tccggaaact
     7081 ccttcgcgaa aaagtgggtc atggtggact tggagaggtc ggcgtccact gccacgacgt
     7141 ccttgttctc gcgtccgagc gccaccagag ccttaccgaa ggcctctcgg gtggccatgc
     7201 ccatcttcag ttcgaacttc gttctggtgg ccattattgc aactccttca gtgccacttg
     7261 cacttcgtcc ggcgtcggcg cgactccgtg aaacttcgga ttgttctcca tgaaagagac
     7321 acctttgccc ttaacggtgt gggcgatgat cgcgactggc ttgcccttga tagcttcggc
     7381 ctccgtgaaa gccgcctgta gcgcgggaat attgtgtccg tcaagctcga tcacgtgcca
     7441 gccgaacgac tgccatttgg ggacgagcgg ctcgacttcc atgatgtcct tgacccagcc
     7501 gtcgagctgg atcctgttgt agtccacgat ggccacaaca ttatcgattt tgtgataggc
     7561 cccgaacatg gccgcttccc agacctgccc ctcctggatt tcaccgtctc ccaggaccac
     7621 gtaggtccgc cacggcttgc cgtccaggcg ggctgcgcag cccataccga gggctagcga
     7681 gagaccctgg ccgagagagc cggtcgaggc ttcgagagcc gggatgaacc gcttgtctgg
     7741 atgtccctga tagggcgagc ccagcttacg aagcgttttt aactggtcga ccggggtgta
     7801 gccgcattcc gccatgacgg catacagcac ggggcagccg tggcccttgg agaggataaa
     7861 acgatcacgg tccggccatt gcgggttggc gggatcgtgc ttcatgacgt cgaaaaagag
     7921 cgtgacaaga atctcgacgg cggacaatga tccgccaggg tgtccggact tcgcggcgcc
     7981 ggtcatctcg atgatgtgac ggcggacgcg cttgcacatt tcctgcagtt cttccaggtt
     8041 tttcttgcgt tccatggttc ccactacaag ctttcaatgt aacatagggg gcggaggcga
     8101 ccatggccgt tgaaattcga aaaatcgact actattacgc gattgttccc gacaaaccgg
     8161 gcgagggagc ccggatcctc tcagccctcc accaggccgg catcaacctg atcggcttct
     8221 ccggatttcc cgctggcgca cggaaagcac agctcgattt cctgcccgag gacagtgcgg
     8281 cattcgccaa ggccgcccgc aacgccggct tgaaactgag caagaagaag gccggactgc
     8341 tgatccaggg ggaggaccag ccgggagccg gcgccgaaat tttcggcaag ctggccgccg
     8401 ccggggtcaa cgtcacctcg ctgcaggccg tttccgcggg cgccggccgt ttcggcggca
     8461 tgctctgggt caaagcgacc gacatgcgga aagccgtcaa ggccctcggc gcggcataag
     8521 aaccggaacc atcagagcag cctggcggcg tctttagcga aatacgtcag gatgatatcg
     8581 gcgccggcgc ggcggatcga ggtcagcgtt tccatcatcg ctcgctcccg gtcgagccag
     8641 ccgttgcggg cggcggcgac gatcatgctg aactcgccgc tcacctggta ggcggcgaca
     8701 gggacgggaa agcggtccct ggcctggcgg atcaggtcga gatacggcat ggccggcttg
     8761 accatgatca tgtcggcgcc ctcctcgagg tcgagttcga tctcgcgcat ggcttcgcgt
     8821 ccgttggccg ggtccatctg gtagctgcgg cggtctccaa attgaggcgc cgactcggcc
     8881 gcttcgcgaa acggtccgta gaataccgac gcgaacttcg ccgcgtaagc gaggatggga
     8941 acgccgggga agccgctcgc atcgagcgcc cggcggatgg cggccacgcg gccgtccatc
     9001 atgtccgagg gagcgacgat gtcggcgccg gcacgggcat gagagacggc ggaggcggcc
     9061 agccactcga gagtggcgtc gttgtctaca tcgtcgttca cgaccttccc gcagtgcccg
     9121 tggctcgtgt actcgcagtt gcagacgtcc gtcaggacca gcagccccgg tacgtcgcgc
     9181 ctgatggcgc gaacagcccg ctggacgatg ccgtcctccg cgtaggcgcc cgaggcagtc
     9241 tcatccttgg tctcagggat gccgaacaga attacgccgc caatgccgag cgaacggact
     9301 tccgcgcact ccttgaccat ctcgtccacg gacatctggt agttgccggg catggcgccg
     9361 atctcgcggc gcacgccttc gccggggcag gcgaacagcg ggaggataaa ctgcgacggc
     9421 gtcagccggg tttctcgcac cagatcccga agcgaggcgg tggcgcggag ccggcgcatg
     9481 cgatgaacgg ggaatgccat atccacggat tgtagcgcgg cgcaatcatg ggggcctgct
     9541 acgataagcg gtgaccatgg gcaggccgaa agactccgcc gcggtggcgc gccgatacct
     9601 tgtgcgcggg cgggtgcagg gcgtaggtta ccgatacttc gtggagcgtg ccgcctatga
     9661 gctcggggtg accggttatg cgcgaaacct cgaagacgga cgtgtcgagg tctacgccgt
     9721 gggtccgccg gaccgccttt ccgcgcttgc gggtttcctg tggaagggcc cgcgcctttc
     9781 aaacgtccgg ggcgtcgaag aactggatgc gccgctccag aaatacgccg gatttcacat
     9841 cgagtaaccc cggcatcgtg agcgccggat gctacaatac gttgttcccg cgaacgatca
     9901 gaccgatgaa caacgaccaa ctaaagaaac tcattcggga ggtgcccgac ttcccgaaac
     9961 cgggtattct cttctatgac atcacgacac tgctgaagga cgcttccggc ctcaaggctg
    10021 tcatcgacgc gtttggcaag cggtaccagg gcagcagtgt ggacgtcgtc ctcggaattg
    10081 aagcacgggg attcatcttc gcgccggcgg tggcatacgc gctcggcgtg ggcttcgtcc
    10141 ccgtgcggaa gccgaaaaaa ttgccggcgg agacggtccg cgaggagtac cagctcgagt
    10201 acggcaccga cagtctggag attcataagg acgccatcca gcccggccag cgggtgctga
    10261 tcgtcgacga tgtcctggct accggaggga ccgcggctgc ggtgacccgg ctgatcgaga
    10321 aactcggtgg caaagtggcc ggactcggct ttgtgctgga actggacttc ctgcacggcc
    10381 gcgacaaagt tgcggggtac gagttgttct ccctgctgca ctacgaaaaa tgagcacggg
    10441 gctgcgccac gcccgcgtcg aagcactcgc caagatcaac cttgcactca aagtcctgca
    10501 caagcggacc gacggctacc acgaactccg gacggtcttc cagaccatct cgctcgggga
    10561 cactctcgag atcgagttca ccccgtcgcg gcgcacgaga atcgagctgg tttcgagggt
    10621 cgacatcccg gacaacctgg tcgcccgcgc cgcgcggtct gtgatggatg cgatccgtgt
    10681 gaccggaacg gtacgcctcc ggctcgtcaa gcgcatccca atgggcgcgg gtcttggggg
    10741 cgggtcgagc gatgctgccg cagtgctcct ggcgcttccc gtgctcgccg gcggccggat
    10801 ccctgccgat caactgatag ggcttgccgc cggtctcggc agcgacgtgc cgttctttct
    10861 ggtcggcggt accgcagccg gcatcggacg gggcacggaa gtctacccgc tcccggacca
    10921 tcctgcgttc ccgggcattt tggtagcgcc tcccatccac gtatcgacgg cggaggcgta
    10981 ccgcgggctc gaccgaaaat tgacatcagt tgttccgccc aatatcatga ataatttcca
    11041 gtcctttgct tggtcaggag ggctggggga gccggggaag ggcacgccag cgtctccggg
    11101 aaacgatttc gaggagtcag ttttccggct ccacccacga ctgaaatcgc tgaaggcgag
    11161 actgtcgcga atcggggcgg cgccggtaat gatgaccggc agcggttcgg cgctcttcgg
    11221 gctcttccgc ggccggaacg atttggagag agcccttccg ctattcgaag aggagaccgt
    11281 gtttcggttc agcctggtga cccgcgaacg ctataggcgt gcctggatgc gctggcttgc
    11341 cgctcacatt gaaggggaga tatggccgcc gcaaagccgg tactcaaaaa caaagacgac
    11401 cggttgaaag ttttcacggg gaacgcgaac ccgtcgcttg ccgctgaact gtgtgcctgc
    11461 ctgggctgca cggtgggttc agcgctggtg aagccgtttt ccgatggcga gatgtacgtc
    11521 cagatccagg agaacgtgcg cggcgcggat gtctttgtgg tgcaaccgac ttgcactccc
    11581 gtcgaacagc atctgttcga gctgttgttg atgctggatg ccttgaagcg ggcctcagca
    11641 gaccgcatca ccgcagtgtt accctattat gggtacgcca gacaggaccg gaaagacaaa
    11701 ccccgggttc cgatttccgc gaaactaatc gccagtcttc tggagcgggc aggggccgac
    11761 cggatactag ctctcgacct tcacgctgca cagatccagg gtttcttcga tgtcccggtc
    11821 gatcatctgt ttgcgacacc ggtgatgatc gactacttta agtctctggg gatcgggaat
    11881 ctgacggttg tttcccccga cgcaggggga gtggagcggg cgcgggcgtt tgccaaacgg
    11941 ctcaaggcgc cgctggcgat catcgacaaa cgccgcgagc agatgaatgt ggcggaagtg
    12001 atgcacatca tcggccgggt tagggggcgg aactgcctga tcgtcgacga tctgatcgac
    12061 acggcgggaa cgctggtgaa cgcagcggcg gccatgctgg atcacggcgc gctgagcgtg
    12121 acggcatgtg cgacccacgc ggtgctgtcc ggtccagcgg tggaccggat cgaggtttcg
    12181 ggcctcaagg aagtggtgct gacaaactcg attccgcttc gggaagatgc gaagcggtgc
    12241 aagcggatcc ggacgctttc cgtggcgcgg ttgctggcgg aagcgattcg atcgatccac
    12301 gaggagactt cggtcagcaa gttgtttgtc tgagtttgat gccgggccac gcagcccacg
    12361 tccatcggac ctgcgacccg gaaaaaggag tttcgaatga ggaaggatat cacggtttcc
    12421 gcgaccgttc gtgatagtcg cggaaagaat gaggcgcgcc gtttacgagt gactgggatg
    12481 gttccggcag tcgtgtatgg cgctggtcag aagccgctct cgctgtcggt tgatccgaag
    12541 caactcttga agatcctcca cagcaagacc ggccacaaca cgattttcag cctgacgact
    12601 ggcgacggcc catcgccggt gatgatcgtg gactggcagt tcgagccggt gaagggtacg
    12661 ctgcttcatg tcgatctcaa gcgaatcgac ttgtcgaagc gtctccgggt ttcggtgccg
    12721 gtgtacacgc agggagagcc gaagggcgtc aagcagcagg gtggcctgtt ggagatcgtt
    12781 aaccgtgaga tggagatcga gtgcctgccg gacgatattc cggaacactt cacggtcgat
    12841 gtcaccgagt tgctcatcgg ccagaacatc cgcgcggcgg acgtgccgat cggcgagtcg
    12901 gtcaagctgc tcagccacgg tgacgctgta atcgcacacg tcgttacgct gcggcatgag
    12961 gaggagaagc cggcggaaga ggccccggct gccgagggag cggctccggc ggaacccgag
    13021 gtgatcaaga agggtaagaa ggaagaagag ggtgaggagt aaccggccgg gcgccgggag
    13081 gagcggaagc cgtggaagcc gattcacgcg ctactccatt ccttctggtc gggttgggga
    13141 atccgggcga gcggtacgag cagacgccgc ataacctcgg gttcctcgtc atcgaccggc
    13201 ttgcggagcg ccatcggatc cgcgtgagcc gcaaagaggc ccgctcgctg acgggtacgg
    13261 gcactgtgga agggatcccg gtcgtactgg ccaagccaca aacttacatg aaccttagcg
    13321 gcggggcggt gggggctttg ctcgcgaagc atggcgctcg ggccgaggat cttgtgctgg
    13381 tttatgatga cctcgatctc ccatggacgg cggttcggat acgtccgaag ggttcgccgg
    13441 gagggcacca cggggtcgaa tcggtaatcc gggcggtggg aacttcggag ttcccgcggg
    13501 ttcgcctggg gatcgccggc tataaggtcg gcgatggtgc ggagtttgtg ctttcgccgt
    13561 ttcggcgggg gcagaaggaa gaactggatg agttgctgga tcacgcggct caggcggtga
    13621 cttccatcat ttccgagggc gtcggaatgg ccatgacgag gttcaatcgt cgcgcccgag
    13681 gtaaacagac tgaggaagaa tgagaatcta cgaagaactc tttattttga agccggatgc
    13741 gaccgaggaa gagatcgacc aggtagtcga gcaacttcgg caggttatca ccggcggcgg
    13801 cggagaggtc gacaagatcg acaagtgggg cctgcggagg ctcgcctatc gggtccacaa
    13861 gtacaacgaa ggcttctatg tgctgatcca attccgcgcc tcgccggaag tcgtgcggga
    13921 actcgagcgg cggcttcgcg tgaccgacct tgcggtgaag ttcctgacgg tccgcatcga
    13981 cgagaagctg aagcggatcg agaagcggaa gaaacagcgc gaaaagcgtg cggccagaaa
    14041 gcctgcggcg ccagtggcgg cgccccaacc gccggcggcg gaagagatca tggctgaagc
    14101 cggagcggct gacgccggaa cggagagaga gtaatggccg aaaccaaggg acgaccggtc
    14161 agtgcctcgc agaggccgac gggcggcgac aaggcgatcg ccgtcggcag aaagcaatac
    14221 ttccggcgga agaaagtctg ccgattttgt gtcgacaaga tcgacgacat cgactacaag
    14281 gacgtgaagc tgctctcggg atttgtctcc gagcgcggaa agatcacccc acggcggata
    14341 tccggcgtgt gcacgccgca ccagcggcgg ctgagcgagg ccgtcaagca ggcccgcaac
    14401 atcgccctgc tgccgttcac cacccaggta taagaggaac ctggaggcag cgcgggagtc
    14461 ccggccggaa tggcggcgcc gggtttcaca cccggtagcc gcgacaaccg gtggcgcgga
    14521 cgaatgcgcg gccacggagg cgaaagagtc atggaagtca ttctgagaga agatatcgag
    14581 aaaatcggac atcgcggcca ggttgtgcga gtcgccgacg gctacgcccg gaactatctc
    14641 ctgccgcggc ggcttgcggt ccaggccacc gatgcgaaca agacgattgt ggagcaggaa
    14701 cggcaggcgc acctgcgccg cgaggcaaaa gaaaagagcg aggccgggga cttggccaaa
    14761 ctgctggccg gcgtgaagat caccatctcg caaaaagcgg gcgagcagga acagcttttc
    14821 ggatcggtga cctctaagga cattgcagag gcgctcgaga agcagaactt caccatcgac
    14881 agacgcaaga tccatctcga tgagccgatc aagcaactcg gggattacaa ggttgccatc
    14941 cggctccacc atgatgtgac cgccgaggtc gatgttcacg taacgaagga agagtagagg
    15001 cagccggcca tggcattctt cgacctgacg ggccacaccg cggtcgtaac gggcgccgcg
    15061 accggaatcg gtgaggcggt tgtccgccgg cttgccgccg ccggagcttc cgtcgcggcc
    15121 gtcgatctgg acatggaagg cgcggctcgg gtagcaggcg acatcgccgg tggcccgggt
    15181 cgcctcgtat tcgcggttcg cgccgacatc tcgagcgcgg agtccgtgaa tcaagcggtc
    15241 gccgaggtcc ttgaccgaac cggccgcatc gacgtgctcg tgaataatgc cggcatcgcg
    15301 ggccgggccg ctccgttatg ggagcaaacc gtggaagact ggcagcgggt gatcgccgtg
    15361 aatatgaacg gcgtcttcca ttgttgccgg gcggtcatca accacatgcg agagcgtcac
    15421 tacggacgga tcgtcaatgt tgcttccatc gccggcaaag aaggcaaccc gaacatgacc
    15481 ggctactcgt cgaccaaggc ggcggtaatc gcattcacca agtcccttgc caaggaagtc
    15541 gccaccgaag ggatttgcgt gaattcggtt gctccggcgg tcgtcagaac acggattctc
    15601 gatcagctta cccccgccca gatcgaatac atgaccgcgc gcattccgat gcgccggacg
    15661 ggcacacccg acgagatcgc cgcggtgatt catttcctgg ctagccccga ctgctcgttc
    15721 gtcactgggc aatgttacga cgcgagtggc ggccgcgcta cctactaatg tgatcctcag
    15781 cccgaatcag taccccagtg ttccggtcta cgatcttctc gtggccgcgg cgaacggtca
    15841 ggcgcccttc gatcatccct tcttaaacgc tctattcgag cgattcgaca gcgctgtccc
    15901 ggatctggtc cggttcggca tggatgatcg gacccatgac ctgatcgatc tggagccgga
    15961 gcttgtagcg atattccgct acaagccgac ttcgctcgcg ctgcccttcc ttatcaaagc
    16021 ggtgcaccgg cagccggatg acctctccga ggagttgatt gacgcgttcc accgcgtcgg
    16081 accattagcc gtcgaaccgt tactcgaact ttaccagcga ctcggtccgg aggaaggtgg
    16141 cgaagtctcg tttacgctgg ctgctctccg ggttagagac cagcggatct ttgacgttct
    16201 catcaaccgg ttgtccgtcg atcccggcga tgccgccatc cagttggggc tctacggaga
    16261 ccccgccgcg aaaccggcct tggagaaact gctccgcgaa ttcgaagcga atggcgacgc
    16321 tccgcctagc acgatttcgg agatcaggtg cgctctccag gatctcgacg agccacgagc
    16381 ggaggaaacc gccgagccgt acgacatctg gtcggactat ccggaagccg cactgccctt
    16441 gttcgagcat ctcagcgagc aggaacgcac gaaatttctc ggcagtccgg tggccgaata
    16501 tcgcgccaga gcggctgaaa gcctcggacc ggaactcagc gctaccgtga aagcgcacct
    16561 gttcgagatg gctcgcaccg atccggaggc gccggtacga ggcgcctgct gggagaggct
    16621 gcgcgatgcg ctccccgata cggatgtgcg ggcggcgatg atcgagcgtc tcagcgacga
    16681 gactgctccg cag
//
LOCUS       DATIFQ010000192        15743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_639974, whole genome shotgun sequence.
ACCESSION   DATIFQ010000192 DATIFQ010000000
VERSION     DATIFQ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639974"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VLE22_08870"
     CDS             <1..352
                     /locus_tag="VLE22_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB14559.1"
                     /translation="VSHCGGISEARRIAAMAETYQVTVACHNPQGPVSTAASTHVGFS
                     VPNYLIQEVVRKDVPWRNDVVTESLPIEGGICRAPTKPGLGVEVNETEAAKHSYEPEV
                     LMAYHRRDGSVADW"
     gene            501..947
                     /locus_tag="VLE22_08875"
     CDS             501..947
                     /locus_tag="VLE22_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB14560.1"
                     /translation="MVRSPRDPRRRILELARVLLVDDNPTSRLTLQTVLEAGGYHVEG
                     AASAAEAVGKLEGSEFELVLSDLHMESPEAGLKVLAHARMMDYKPATALIATYQDSPG
                     RTNSRKGNQAVLIESEDLPELLSKVAQLISERATRRVERELRHAGV"
     gene            complement(928..1575)
                     /locus_tag="VLE22_08880"
     CDS             complement(928..1575)
                     /locus_tag="VLE22_08880"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HSB14561.1"
                     /translation="MPRRLESLMLDGPAGRLEALLEEPENQDPAEVALVCHPHPRHGG
                     TMHNKVVYRIARGLRRLGAVVLRFNFRGVHLSEGTYDHGEGEVEDAHAALDYVLGRYP
                     GLPYTLAGFSFGSRVALRVACSRTDAGRVIAVGFPTVYRDKDALGLCKVPKIFIQSTG
                     DEHGPPPAVREFFERLKEPKKLVWVDARDHFFGGALDDFEEAVYRLGPAVKPRRV"
     gene            complement(1575..2075)
                     /locus_tag="VLE22_08885"
     CDS             complement(1575..2075)
                     /locus_tag="VLE22_08885"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HSB14562.1"
                     /translation="MPDNSFDIVSKIVLAEVNNAIQQSLKEIGTRFDLKNSHSTIELN
                     EKDKKVLLASQDEFKLKAVIDILEQKLVKRKVPLKGLTYGQVTPAAGSTVRQDISLQQ
                     GIPIEKAREIVKRVKESKLKVQASIQGDFVRISGRDRDVLQAVIRKLNSEDFGIDMQF
                     TNYRTN"
     gene            complement(2128..2742)
                     /locus_tag="VLE22_08890"
     CDS             complement(2128..2742)
                     /locus_tag="VLE22_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB14563.1"
                     /translation="MSRVLLADDSPHAQRMGEWILREEGFEVVTVTDGESALVRLPDV
                     DPDVILADVFLPQRSGFEICRRVKTDPARRHTRVVLTAGALEPLDEEETKRVQADATL
                     KKPFEASMVIDIVRPLILAAQADREAMRVARAEQTAASSLGQHEEPPAAPQPELPVAA
                     SPLGDAAQEVDPERVRAAVTVALDAAFPSLIEEITEKVLHSLRK"
     gene            complement(2755..3924)
                     /gene="bamD"
                     /locus_tag="VLE22_08895"
     CDS             complement(2755..3924)
                     /gene="bamD"
                     /locus_tag="VLE22_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HSB14564.1"
                     /translation="METKARLVLERIALAVLAAGLLVTLGCGRKKYENPITKDTLQPD
                     KVLFDKAIKDIEKGRYDIARLTLQTLISTYDTSEFLAKAKLAVADAWFREGGSHGLAQ
                     AEAEYKDFILFYPTLEESAEAQEKVCMIHYRQMEKADRDTTHALRAEDECRQVLVQFP
                     NSKFAPRAQQLLRNIQEVLAEHEYRVGVFYHTKGSNPSAANRLQSLTDHYPLFSKADD
                     SLWRLGDAYSKMGPRFRDRSVQAYSRIVREYPLSEYVDEAKKLLQSMEAPVPEPDPVA
                     LARMKYELENYDKPGMMSHFWGIFRKSPDTSPAAKSGAPSMAAMRPGVPLSVPAAAGG
                     PGAVTADVTVSTVGGDSSALDTKPDARLSAPAPAQDPQQASGQAESKKKDQKKKK"
     gene            complement(3948..4622)
                     /gene="rpe"
                     /locus_tag="VLE22_08900"
     CDS             complement(3948..4622)
                     /gene="rpe"
                     /locus_tag="VLE22_08900"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HSB14565.1"
                     /translation="MLEIIPSILSADFSRLGEEIAKVERGGATMLHVDVMDGHFVPNL
                     TVGPPVLRSIRKVTKLTLDVHFMITNPDPYVPLFVEAGADQISVHQEACPHLDRTLRL
                     IQSEGALAGVVLNPSTPATMLEEVFGLADFVLIMSVNPGFGGQQLIPRTLEKVRWVAT
                     RRREMGLKFPIEIDGGVTLENLSEVVRVGVDWVVAGATVFLSPDPAATVVEMRRIAQE
                     SNAVRA"
     gene            complement(4632..5777)
                     /locus_tag="VLE22_08905"
     CDS             complement(4632..5777)
                     /locus_tag="VLE22_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSB14566.1"
                     /translation="MSPARHVAFRILLRIPAGAHASDLLRRSDGLDARDAGLASEIVF
                     GCLRMQAQLDALIARASGRPAERLDPEVRIALRIGIYQLRYLDRIPRHAAVAESVGLV
                     RTARKSSAAGFVNAVLRKIDRGPVAWLDRVTALSHPAWLLESWTRQFGASTAERIALA
                     FLKPPETWVRVPQGVSVEGVELEPCGPPGCYRVVSGEPSRLRIQDIGSQSIIPLLELE
                     SGATFLDLCAAPGNKTAQALEQGVQAVACDISPLRLAALKGLRCGRVVADVSEPLPFR
                     RRFERILLDAPCSGTGTLGRNPEIKWRLQPSDLPRFHDRQVKMLTRALDALAPGGRLV
                     YSTCSLEREENEAVLEKVLGRLPDKILRRLPGVDPGDGFFAAVIPSN"
     gene            6354..6764
                     /locus_tag="VLE22_08910"
     CDS             6354..6764
                     /locus_tag="VLE22_08910"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB14567.1"
                     /translation="MLVVVPPDGDRRRELRFKVERPCRVYPADAGLQKVDGVIEDISR
                     TGIRIFLPDSRMSQRWPKVGDAALIVLDLPQSKKYAPRCLECAGRVVRVGNALADWSS
                     FAFEIERVRVRSNGKRKCDKSVELLEMNPTARTQ"
     gene            6773..9379
                     /locus_tag="VLE22_08915"
     CDS             6773..9379
                     /locus_tag="VLE22_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSB14568.1"
                     /translation="MDSAIDQYGKWAVAAQSLPEGPPLSDEQTYRELFENANDVVYTH
                     DLQGNFTSVNKAIQRITGFSRAEALKLRIQDLLVPQSADTAQRMLTKMLGGAPRATYE
                     VSFRTKDGRAVAFEVSSRLLFRQGQPAGVLGIARDVSQRRRSEAHLHLLNSVVVNAND
                     AVLIAEVQPGDSLGARIVYVNEAFTRMTGYIADDAIGRTMRILLGPRTERERLDEVRR
                     ALANLAPVRIEMVNYRQDGSEFWVDANFVPIVDDDGEFRRWMAVQRDITSRKRAEELE
                     RDRNHALEMMANNEPLEKLLGHLVEMVERQHPDLRCSVMLSDDEKSFAGEPEAAVSAV
                     AGGVRTAGTPAPTQGRIEILAGNGSSLGYFKVQCKTPRALSEDEFDLVRKAGRLAAIA
                     IEQRQLTERLAHQARHDALTGLPNRFLFEDRLQQALARARRHGTLTAVLFVDLDRFKQ
                     INDTLGHAAGDALLQQVAHRLSLCIRETDTLARMGGDEFTVLLGDLRETRYALVVAQK
                     LLDELKSPFSVEEYELFVTASVGISLFPRDGRDSATLQRNADSAMYRAKNQGRNRYQC
                     FVPEIGETAQESLEIETALRKAVENGELLIQYQPQVDHLGRVVGLEALLGWHHPRLGM
                     ISPTQFIPVAEETGLIFPIGGWVLEHACRQSVAWQKAGYEGVKMAVNVSAVQFNRAGF
                     VDTVAETLQRTGLDPSMLELELTESVVMRDVRESSRQMQGLRTLGVSLSIDDFGTGYS
                     SLSYLRRLPIDTLKIDQSFLREMDGEPSTMPLVKAIVALAHSLSLSVVAEGVETERQL
                     GALRRVGCDRFQGFLLGQPMPAGPAERWLQSTTDALALLPRIGPGRDSDRTSVATSIE
                     SLFVPLTPAAAS"
     gene            9671..10720
                     /locus_tag="VLE22_08920"
     CDS             9671..10720
                     /locus_tag="VLE22_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14569.1"
                     /translation="MSDQKLVCPHCGHDGSAESAKSPLESFGFNYLEDDVVSREVRGF
                     DADGKLLIDREPRATGARGMNPRIECRSCWQTFTLPAEVERAAQPEVVVGLESAEVSP
                     QGPQPEVVPTPPPAPAVARSVAEKLADFFETALNEIRQTLASDLEKIEGRVANVTTGL
                     GDVQNGLNTVRLRTEDLTEEQHREAEAVSEIEARLRRFEDGMPALREDVQALTAGQMD
                     VRDEIETQLHRVVGIEDGVSRVEQLVQQYREALDLNANSARAEFAALKDRCEALERRL
                     ADETGRAESLRKELRESQEAELRRLDAQAAAIRSLHLSAEAQLRHREELRATLQKLET
                     LAQATDIPSPLPENL"
     gene            10819..11913
                     /locus_tag="VLE22_08925"
     CDS             10819..11913
                     /locus_tag="VLE22_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14570.1"
                     /translation="MATFDFVTSGEFRASLERDAEELLACMKAGAWKSVHVLAGSLIH
                     ATILYHLMSTGKVGENELLKLSLSELLDLCKEQQVLSTRTLDLAVFIRPYLQFIHPSA
                     GTRLCEGPDETGARIAQALLEIIVNELSANRRQSYGVTAEQVVAKLQLDPSSIAILDH
                     LLRKMNEQELERLLIEAIPNAYLDGARFQPQEKEAALQHLARCFRMALALASDGLKRK
                     VVKRYIGVLEHESEYTVRAYETQFFRGTDLKFLDAEEREVVKRHFVSSLSRNFSPELV
                     EAAEGMGAFLESEEEARAFFVPLVLRMIDEKDETQRTAIRDRVLNEYRITSEQNQQML
                     RSWTARLKGFLRREGREAAVEVMLPLEAVF"
     gene            11989..12780
                     /locus_tag="VLE22_08930"
     CDS             11989..12780
                     /locus_tag="VLE22_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB14571.1"
                     /translation="MDLERTLLRKVGEAIGRYRMIRDGDRVAVALSGGKDSLTLLEIL
                     IRLRDRAPVDFSVCAFTVEQGKFLRPIEPLGNYLRERGIAWTYFQDKASLRLVEEQPG
                     HGCDLCSRYRRRAVYEIARGLGANVIAFGHTADDFCETFLRNSLFTGRVSALPPVTHS
                     RDREFRLIRPLLFVPESLTQQFTESLGAPVVPCGCSQKTGTVRRALRDIFASLEKDYP
                     NLKETMLAAMGNLEPARLLDTRYLDLDDGRAEAVERNPFPVLPES"
     gene            12861..14501
                     /locus_tag="VLE22_08935"
     CDS             12861..14501
                     /locus_tag="VLE22_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14572.1"
                     /translation="MLSRLLQSFRMRNLPGLSRRDLFRGGGLMAAAGLFEGSVQSAAA
                     DAGLRIGPDIYQSIGVHPIINCKGTFTIISGSLSLPEVKQAMDAASRHYIHLDELMDA
                     VGKRLAEITGAEFGVVTAGCAAALTHATAACIAGADPEKLQRLPYLEGLKNEVLVPRY
                     SRNVYDHAVRMLGVKIVEADNLDEFEKAFNERTAMVMVLASPPDSGPFGLEPICRIAR
                     LKRVPVLVDAAAEKLTIPNVHLQRGAHLVAYSGGKCLRGPQCAGVLLGQKDLVKAAWL
                     HSAPHHAFGRSLKVGKEEIMGMLAAVEMWVKRDHDAEWRQWESWLDEIAGQVTKVDGV
                     TTDVLQPSGLSNYAPQLRIKWEGEKIGITGREMEKILWEEEPRIILSGASGDYRDNMA
                     SSIVIMPYMMTPGDAQVAGKRFRELLEKPPRVERKRNTDPPVQISGQWDVHMEFFCGS
                     GEHSLVFEQKGSELVGTHSGDILSGDLRGTMEGNEVRFRSSHKYEGTRLGYRFEGKVD
                     GDIMRGTVDMEEYGVAQWTAHRHTYGRPGGIVRPQKNV"
     gene            complement(14531..>15743)
                     /locus_tag="VLE22_08940"
     CDS             complement(14531..>15743)
                     /locus_tag="VLE22_08940"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HSB14573.1"
                     /translation="GIRNRDGKEITEGEIRKLADSGIREMTLHNDGDAFQDGIFWRDG
                     SYPPYPPDVMRKMDAMIDTLHRHGIRLSPYFSNHELHQSTDEFKKHGEEWGRKPDDQD
                     NQRPNTFYGSHMCLKSSWLDFMKLCVDRVLKNHKFDGIYYDWNIAMFCNNPRHVGKTS
                     NGVSGEKGLAALAISETGHWDIDELLELVEWTRERVGPNGLFILHNTLVPMFATENFA
                     DKVVGLEFGYARLSVSVPPPSELPLEWDWAGARSRAAIIIGTVDNNAPKRILRQHALL
                     GLMTAVMPWRANEETIEFAARLKPLGNLEDYKFEDYRNNAVKIEGEKLVTAVYSKQGE
                     AYLLLANFNAEPKTVTCVVRPGNLPSPLSSVRSAKVVSGATASVDARRLTAGGALVTI
                     PADDVVVLQLK"
BASE COUNT         3196 a         4505 c         5066 g         2976 t
ORIGIN      
        1 tgtctctcat tgtggcggta tcagcgaggc aaggcggatc gcggcgatgg cggaaacgta
       61 ccaggtcacg gtcgcctgtc acaatccgca aggacctgtg agcacggctg cttctacaca
      121 tgtaggattt agcgtcccaa actacctgat ccaggaggta gtgcggaagg atgtaccatg
      181 gcggaacgac gtcgtgacgg agtcgctgcc catcgagggg gggatctgcc gtgcgccgac
      241 gaagcccggg cttggtgtgg aagtcaatga aacggaagcg gcgaaacact cttacgagcc
      301 ggaggttctg atggcatatc accgccggga tggttcggtg gccgattggt agccagaaga
      361 cgacggtacc tgcgagaagc atcactataa aaatagaaaa ttaacagggg gcaaccttca
      421 caaaagtgac gcaaattccc tgccggtttc ccggtagttt ggggtatagt tagggtaccg
      481 agaaaggact acggtagtaa atggtaagaa gcccccgcga tcccaggcgc cggatcctag
      541 agttggcccg tgtcctgctg gtggacgaca atccgacgtc acgcctgacc ctgcagacag
      601 tactggaggc cggcgggtac catgtcgagg gtgctgcgtc agccgcagag gcggtaggca
      661 agttggaagg atcggaattc gagcttgtgc tcagcgatct gcacatggaa tcgcccgagg
      721 ccggattgaa ggttctcgct cacgctcgga tgatggacta caagcctgcg acggcattga
      781 ttgcgaccta tcaggattcg ccagggcgaa ccaattcgcg caagggaaac caagcggtgc
      841 tgatcgagtc cgaagatctg cctgagttgt tgagcaaggt cgctcagttg atcagcgagc
      901 gggctaccag gcgagtggag cgagaactca gacacgccgg ggtttgacag ccggtcccag
      961 ccggtaaacc gcctcctcaa agtcatcgag ggcacctcca aagaagtgat cccgggcatc
     1021 tacccaaaca agctttttgg gttccttgag tcgttcgaaa aactcccgta ccgccggagg
     1081 agggccatgt tcgtccccgg tgctctggat gaagattttc ggcaccttgc atagtccgag
     1141 agcatctttg tcgcggtata cggtggggaa accaacagcg atgacacggc ctgcgtccgt
     1201 ccgcgaacag gcaacgcgaa gggcgacacg ggatccgaac gagaagccgg ccagggtgta
     1261 cggcaagcca ggatagcggc cgagcacgta atcgagagcg gcatgggcgt cctcaacttc
     1321 gccctctccg tgatcgtacg tcccctcgct taggtggacg ccacggaagt tgaaccgaag
     1381 caccaccgcg ccaaggcgcc gcagacctcg cgcgatacgg tagacgactt tgttgtgcat
     1441 ggtaccgccg tgccggggat gcggatggca gaccagtgca acttccgccg ggtcttggtt
     1501 ttctggttcc tccaggagcg cttcgagcct gccggcggga ccgtcgagca tgagcgactc
     1561 taggcgccgc ggcatcagtt cgtccgatag ttggtgaact gcatgtcgat cccaaaatct
     1621 tcggagttga gcttgcggat gacagcctgg agaacgtcgc ggtcgcggcc gctgatgcgg
     1681 acgaagtcgc cttggatcga ggcctgcacc ttcagcttgg attccttgac gcgtttcacg
     1741 atctcccgcg ctttctcgat cggaatcccc tgctggagcg agatgtcctg gcggacggtg
     1801 gaaccggcgg ccggcgtaac ctgtccgtag gtgaggcctt tcagcggcac ctttcgcttg
     1861 acgagtttct gttcgagaat gtcgatgact gctttcagct tgaattcatc ctggctggcg
     1921 agaaggactt tcttgtcttt ctcgttcagc tcgatggtcg agtgggagtt cttgaggtca
     1981 aagcgggtgc caatctcctt gagagactgc tgaatggcgt tgttcacttc cgccagaacg
     2041 attttggaga cgatatcgaa gctattgtcg ggcatcagta gatccggagc cttgatggtt
     2101 ggatgattcc gggatagatg gagacgctca tttccgcagg gagtgaagga ccttctccgt
     2161 gatctcctcg atcagagagg gaaacgcagc gtcaagagct acggtcacag cggcacggac
     2221 gcgctcgggg tcgacctcct gtgcggcatc ccccagtggt gatgccgcca cgggaagctc
     2281 gggctgcgga gccgcaggcg gttcttcgtg ctgccccagg gaagacgctg ccgtctgctc
     2341 ggcgcgggcg accctcatcg cttcccgatc cgcttgcgcc gcgaggatca gcggccggac
     2401 gatgtcaatg accatagaag cctcgaacgg cttcttcaac gttgcatctg cctggacgcg
     2461 cttcgtctcc tcctcgtcga gcggttcgag cgcgccagcc gtcaagacga cgcgggtatg
     2521 tcggcgcgca gggtccgtct tgacacgacg gcagatctca aaaccggagc gttgggggag
     2581 aaaaacgtcg gcgaggatca cgtccggatc gacatcgggc aggcgaacga gagcgctttc
     2641 gccatcggta acggtaacta cctcgaatcc ctcctcgcgc agaatccatt cacccatgcg
     2701 ctgggcgtgg gggctgtcgt cggcgaggag gacgcggctc atccgatggg agccctattt
     2761 cttctttttc tggtccttct tcttcgattc cgcttggccg gaggcctgtt gcgggtcctg
     2821 cgcgggcgcc ggagcgctca atcgggcatc cggtttggtg tcgagggcgg aggagtcgcc
     2881 gccaaccgtc gagacggtca cgtcggcggt aaccgcaccc ggacccccgg cggccgccgg
     2941 cacgctcagc gggactccgg gacgcatggc ggccatggac ggagcgccgg acttggccgc
     3001 gggcgaggta tcgggactct tgcggaaaat gccccagaaa tgactcatca tcccgggctt
     3061 gtcgtagttt tcaagctcgt acttcatgcg ggcgagcgcg acggggtcag gctcgggaac
     3121 cggggcctcc atcgactgga gcagcttctt cgcctcatcc acatattcgc tcaggggata
     3181 ctcgcgaacg atgcgagagt aggcttggac actcctgtcg cggaagcggg ggcccatctt
     3241 ggagtaagcg tcgccgagcc gccacaaaga gtcgtccgcc ttgctgaaga gagggtagtg
     3301 gtcggtgagg gattgaagac ggttggcggc ggagggattg ctgcctttgg tgtgatagaa
     3361 cacgccgaca cggtactcgt gttcggcgag cacttcctgg atgtttcgca gcaactgctg
     3421 ggcgcgggga gcgaacttgc tgttcggaaa ttgcacgagg acctgccggc actcgtcctc
     3481 ggcgcgtagt gcgtgagtgg tgtcccgatc cgccttttcc atctgccggt agtgaatcat
     3541 gcagactttt tcctgcgcct ctgcggattc ctcgagagtg ggatagaaga ggatgaaatc
     3601 cttgtactcg gcctccgctt gcgcgagacc gtgcgaaccc ccttcacgga accaggcgtc
     3661 ggcgacggcg agcttggctt tcgccagaaa ctcgctggtg tcgtaagtac tgatcagcgt
     3721 ctgcaacgtg aggcgggcaa tgtcgtaacg acccttttcg atatccttga tcgccttgtc
     3781 gaagaggact ttgtcgggct gcagtgtgtc tttcgtgatc gggttctcat atttctttct
     3841 cccgcatcca agagtcacga gcagcccggc cgccagcacg gccagagcga tccgctccag
     3901 aactagcctt gcttttgttt ccataagaca ccaacactcg ccgggcgtca tgcccggacg
     3961 gcattcgact cctgtgcgat ccttcgcatt tccacgaccg tggcggccgg atcggggctc
     4021 agaaacactg ttgctcccgc gactacccag tcgactccca cgcgcacgac ttccgaaaga
     4081 ttctccagcg tgactccgcc gtcgatctca atcgggaatt tcagtcccat ctcgcggcgc
     4141 cgggtcgcga cccagcgtac tttctccagc gtccgcggaa tcagttgctg gccgccaaaa
     4201 ccgggattca cgctcatgat cagcacgaag tcggccagcc caaacacctc ttccaacatt
     4261 gtagcgggcg tcgacggatt cagcacgacg ccggccagcg caccctcgct ctggatgagc
     4321 cggagcgtcc ggtccaagtg aggacacgct tcctgatgga cggagatctg gtccgcaccc
     4381 gcttcaacga agagaggaac gtacgggtcg ggattggtga tcatgaagtg cacgtcgagg
     4441 gtgagcttcg tcaccttccg gatagagcgc aggaccggag ggccgaccgt caggtttggc
     4501 acgaagtggc catccatgac gtcgacgtgg agcatcgtgg ccccgccgcg ctcgactttg
     4561 gcgatctcct cacccagccg cgagaagtca gcggacagga tggacggtat tatttccaac
     4621 atgttgcggg actagttcga tggtatcaca gcggcgaaga aaccgtcgcc tggatcgacg
     4681 ccaggaagcc ggcggaggat tttatccggc aggcgcccaa gaaccttctc aagtactgcc
     4741 tcgttctcct cccgctcaag cgagcaggtg gagtaaacga gccgtccacc gggcgcaaga
     4801 gcatcgagcg ctcgggtcag cattttcacc tggcggtcat gaaacctcgg caggtcgctg
     4861 ggctgcaagc gccacttgat ctccgggttc ctgccgaggg tgccggtgcc cgagcaggga
     4921 gcgtcgagga gtatccgctc aaaacggcgg cggaacggca gcggttcgga gacatcggcg
     4981 acgacacggc cgcagcgcag ccctttcagc gcagccaatc tgagcgggct gatgtcgcaa
     5041 gcgacggctt ggacgccttg ttcgagcgct tgcgccgttt tgttaccagg ggccgcgcaa
     5101 aggtccagaa aagtggcgcc cgattcgagt tcgagcagcg gaatgatgga ctgtgaaccg
     5161 atgtcctgaa tacgaaggcg ggacggctcc ccggaaacca cgcgatagca gcccggcggg
     5221 ccacatggtt ccaattcgac accttcgacg gacactccct ggggaacgcg gacccacgtt
     5281 tcaggaggct tgagaaatgc gagagcaatc cgctccgcag tggatgcgcc gaactggcgc
     5341 gtccaacttt cgagcaacca cgcagggtga gacagggcgg tcacccggtc cagccatgcg
     5401 acgggtcccc tgtcgatttt tctgagcacg gcgttgacga aacccgccgc ggaggatttt
     5461 cgcgccgtcc gcacgaggcc gacactttcg gcgacggcgg cgtggcgggg gattcggtca
     5521 aggtatcgca gttggtagat tccgatccgg agcgcgattc gcacttccgg atcaagccgt
     5581 tccgcgggcc ggccggaagc gcgtgcgatg agcgcatcca gctgcgcctg catgcgaagg
     5641 catccaaaga cgatttcgga tgccagcccg gcgtcccgcg cgtcgagtcc atcggatctg
     5701 cgaaggaggt ccgatgcgtg tgcaccggcc gggatgcgca gaagtatgcg aaaggcaacg
     5761 tggcgcgccg gagacatggc tttctccgtt atcccacgat cgcccgcgga tcgcgagccg
     5821 gccggccggc agacgtttac agagcgcagc gcttacgagg cgctccggtg cactttcgga
     5881 tcgcgacggc gacggtgacc cgtcgaactt ccacggcgac ctcgctcgta ggcctgcaaa
     5941 gagcggatca gggcatcgat ggttcttcgc cggcggtata ggcgaaccag ttcgcgggag
     6001 tacgtcccag acggacccgc ggagggggag gtcggaatgc gtgtcattgc gctagggtac
     6061 cctgaagcgc agaaaagtac cacgggacga aagtaacatt cccattgcgc aaatgagggt
     6121 tccggagtgg gactgtacta tccatcggaa taccgcgcca ttaactttga ttggtgacag
     6181 tattcgtaac ctactgattc tccgtgggaa cgccagcagc cgcagtcgcg gatcacacct
     6241 ctaacctcgg ttaatggtgt actggcatcc cgtaccacgt agactgtccg aaagataggg
     6301 aagtaaggac gctaagtacc tttatattct tatatatgca gcagccgagc tgtatgttag
     6361 ttgtggtccc tcccgatggc gaccggagac gtgaactgcg ttttaaggtc gaacggccgt
     6421 gccgggtgta cccggcggat gccggactcc aaaaagtgga cggcgtaatc gaggacatca
     6481 gccgaacagg cattcggatc tttctgccgg atagccggat gagccagcga tggccgaagg
     6541 tgggagacgc ggccctaatc gtgctcgacc tcccgcaaag caaaaagtac gcaccgcgtt
     6601 gcctcgagtg cgcaggccgc gtggtccggg tcgggaatgc acttgcagat tggtcatcct
     6661 tcgcattcga aatagagcgc gtacgcgtgc ggagtaacgg aaagcggaag tgcgacaaat
     6721 ccgtggagct gctcgaaatg aaccccacgg cccgaactca gtagggagaa gcatggactc
     6781 ggcaatcgat caatacggaa agtgggcggt tgccgcgcag tctctgccag agggtcctcc
     6841 gctgagcgat gaacagacat accgcgaact tttcgagaac gccaacgatg tcgtctacac
     6901 gcacgacctc cagggcaact tcacctccgt gaacaaggcc atccagcgca tcacaggttt
     6961 cagccgggcc gaagcgctga agctcaggat tcaggacctt ctggttccgc agtcggcaga
     7021 caccgcacag aggatgctga cgaagatgct gggcggcgcg cctcgagcca cctacgaggt
     7081 gagctttcgg acgaaagacg gccgcgccgt tgccttcgag gtcagctccc ggctcctgtt
     7141 tcgccaggga cagccggctg gagtgttggg gatcgcccgg gacgtcagcc aaaggcggcg
     7201 atccgaagcc cacctgcacc tgctcaattc ggtggtcgtc aatgccaacg acgcagtgct
     7261 gatcgcggag gtgcagcccg gagacagtct cggcgcgagg attgtctacg tcaatgaagc
     7321 gttcacgcga atgacgggtt atatcgccga cgacgcaatt gggcgaacca tgcgtatcct
     7381 gcttggtccg cgcacggaaa gagagcggct cgacgaggtg cgccgggcgt tggctaactt
     7441 ggccccggtt cgaatagaaa tggtgaacta ccgccaggat ggttctgagt tctgggtgga
     7501 cgccaatttc gttccgattg tcgacgacga cggagaattc cggcgctgga tggcagtcca
     7561 gcgagatatc accagccgga agcgcgctga ggaactggag cgggatcgca atcacgcgct
     7621 ggaaatgatg gcgaacaacg agccgctgga gaagctgctc ggccacttgg tcgagatggt
     7681 tgagcgtcaa catccggatc tgcgctgctc tgtgatgctc tcggacgatg aaaagagctt
     7741 cgcgggagag cccgaagctg cggtgtcggc tgtcgccggg ggagtgagaa cagccggcac
     7801 gccggcgccg acgcagggga ggattgagat cctggcaggg aacggctcct cgctgggtta
     7861 cttcaaggtt cagtgcaaga cgccccgagc tttgagcgag gacgagtttg acctggtgag
     7921 gaaagcaggc cggttagccg cgatcgcgat cgagcagcga cagttgaccg aacgcctcgc
     7981 gcaccaggca cggcacgacg cgctgacggg cctgccgaac cgtttccttt tcgaagaccg
     8041 ccttcagcag gcgctggccc gcgcacggcg gcacggcacg ctcacagcgg tgctcttcgt
     8101 cgacctcgac cggttcaagc agatcaacga caccttgggc cacgccgccg gagacgcgct
     8161 gctccagcag gtagcgcacc gcctttcgct gtgcattcgc gagacggaca cactcgcgag
     8221 gatgggcgga gacgagttca ctgtgctgct cggcgaccta cgcgagacgc ggtatgcgct
     8281 ggttgtggcg cagaaactcc tggatgaact caagagtccg ttttcagttg aagagtacga
     8341 actttttgtc acggcgagcg tgggaatcag cctgtttccg agggacggtc gcgactccgc
     8401 cactctccaa cggaacgccg acagcgcgat gtaccgagcg aagaaccaag gccggaaccg
     8461 gtatcagtgt ttcgttccgg agatcggcga aactgctcag gagtcgctgg agatcgagac
     8521 ggcgctgcgc aaggcggtgg agaacggaga gctgctgatt cagtaccaac cgcaggtcga
     8581 tcatttaggg agggtagtgg ggctggaggc gctgctggga tggcatcatc cgcgcttggg
     8641 catgatttct ccgacccagt tcataccggt cgcagaagag accgggctga tcttcccgat
     8701 cggcgggtgg gttttggagc acgcctgccg gcagagcgtt gcctggcaga aggccggcta
     8761 tgaaggtgta aagatggcag ttaacgtctc ggcggtgcag ttcaaccgcg cgggcttcgt
     8821 ggatacggtg gctgagacgc tgcagcgaac gggactcgat ccgtcgatgt tggaactcga
     8881 actgacggag agcgttgtga tgcgcgacgt tcgagagtca tcgcgtcaga tgcaaggatt
     8941 acgtactcta ggcgtgagtc tgtcgattga tgattttggc acaggctact catcgttgag
     9001 ctacctgcgc cggctgccga tcgatactct caagatcgac caatccttcc tgcgggaaat
     9061 ggacggtgaa ccgagcacga tgccgctggt gaaagcgatt gtggcgctag cccacagcct
     9121 cagtctttcg gtggtggcgg aaggcgtgga gacggaacgt caattgggag cattgcggcg
     9181 cgtgggatgc gaccgatttc agggtttcct gctcgggcaa ccgatgccgg ctggccccgc
     9241 tgaacggtgg ctgcaatcca caacggacgc gctcgcgttg ctaccgcgga tcgggccggg
     9301 gcgggacagc gaccgaacct cggtggcaac ctcgattgag tcactgtttg tccctctcac
     9361 tccggcggcg gcgtcgtaga ctgtgccccg ccctgcttga ccttcaccag aatggaactg
     9421 tactagaacc ctcaccagaa tggaactgta ctagacaact caatcgttcg ggctagtaca
     9481 atctgcaacc caaatagccc cggaagctat ttacttaaag tcacttaggc tgctacactg
     9541 ctgctggccg cgactgtgtc gacggtcgac ttgaggacgt gctcagtgcc cgaccgggca
     9601 agagaggctg cgctccgaac ggggaagggc gcagaatcgt cgtgccggcg ggccgacacg
     9661 ggaggtgagt gtgtctgacc agaaattggt ttgcccgcac tgcgggcatg acggcagtgc
     9721 cgaatccgca aaatctcctc tggagagttt tggatttaac taccttgaag acgacgtggt
     9781 gtcccgagag gttcgaggat tcgatgcgga tggcaaactt ctgatcgacc gagagccgag
     9841 agccaccggt gcacggggga tgaacccgag aatcgagtgc cggtcctgct ggcagacgtt
     9901 cacgctgccc gccgaggtgg agcgagcggc gcagccggaa gtggttgtag ggcttgaatc
     9961 ggccgaggta tcgccacagg ggccacaacc ggaagtggtg cccacgccac cgcctgcccc
    10021 agccgtcgca cgcagcgttg ccgagaaact ggccgatttc ttcgaaacag ccttgaacga
    10081 gattcgacag acgctcgcat ctgacctcga aaaaatcgag ggcagggtcg cgaacgtgac
    10141 cacaggcctg ggggacgtgc aaaatggcct taatacggtc cggttgcgca cggaggatct
    10201 gactgaggaa cagcatcggg aggccgaggc agtcagtgaa atagaggccc ggcttcggag
    10261 gtttgaagac ggaatgcccg ccctacgaga agacgtccaa gcgctcacgg ctggccaaat
    10321 ggacgtccgg gacgagatcg agacccagtt gcatcgagtc gttggaatcg aagacggtgt
    10381 atcgagggtg gagcaactcg tccagcagta tcgtgaagcc ctggatctga acgccaactc
    10441 ggctagggcg gagtttgcgg cgttgaagga tcgttgcgag gccctggagc gccgcctcgc
    10501 ggatgagaca ggaagggctg agagcttgag aaaggagctt cgggagtcgc aggaggcgga
    10561 attgcggcgt ctcgacgccc aggcggcagc catccggtca ctgcacctct cggcggaggc
    10621 ccagcttcgc catagggaag aactgcgggc cacactgcag aagctcgaaa cgcttgcgca
    10681 agcgacggat attccgtcgc cgcttccgga gaacctctga gccggagtcg gatgaaagat
    10741 cggatgaaag aaaagacgtg agcggacgcc ggttgtgatg ttgtgaaccg ttcttctggg
    10801 gaggagaacg agcgggaaat ggctacattc gatttcgtca cgtcgggcga gttccgggca
    10861 tcccttgaaa gggacgccga ggaattgctg gcgtgcatga aggccggggc ctggaagtca
    10921 gttcacgtcc ttgccgggag tctcatccat gcgacgatcc tctaccacct catgtcgacc
    10981 ggcaaggtgg gagagaacga gctgctgaag ttgagccttt ccgaactact ggacctctgc
    11041 aaggaacagc aggtgttgtc cacacggacg ctcgacctcg cagtgttcat tcgaccatac
    11101 ctccaattca tccatccgag cgctgggacg cgactctgcg aggggcccga cgaaacgggc
    11161 gccagaatcg cacaggcgtt acttgagatc atcgtcaacg aattgtcggc gaaccggcga
    11221 cagtcgtacg gcgtgacggc ggaacaggtc gtcgccaagc tccaactgga cccctcctcg
    11281 atcgccatcc tggaccacct gctccggaag atgaacgagc aggaactgga gcggctcctg
    11341 atcgaggcaa tccccaacgc gtatctagac ggagcacggt tccagccgca ggaaaaagag
    11401 gccgccctcc agcatctggc acgttgtttt cggatggcgc tggcgctggc gtccgacggc
    11461 cttaagcgga aggtggtcaa gaggtacatc ggcgtcctgg agcatgagag tgaatacacc
    11521 gtgcgcgctt acgagacgca gttcttccgg ggaaccgacc ttaagtttct ggacgcggag
    11581 gaaagggaag ttgtcaagag gcactttgtg tcgagcctca gcaggaactt cagcccggag
    11641 ctggttgaag cggcggaggg gatgggcgcc tttctcgaat ccgaagagga agctcgggcg
    11701 ttttttgtcc ctctggtttt gcgaatgatc gacgagaaag acgagacgca acgaactgcc
    11761 atccgtgacc gggtcttgaa tgagtaccgc atcacctccg aacagaacca gcaaatgctg
    11821 cgaagctgga cggcacgctt gaagggtttc ctccgccgcg agggccgaga ggcagccgtg
    11881 gaggtcatgc ttccgctcga agcagtattc tgatcagcca aaccccggcc cgtacgtgcg
    11941 acaagccact accggcgagt tccggagtca aatcgaccat aatgggattt ggacctcgaa
    12001 agaactctgc ttcggaaggt gggtgaggcg atcggccgtt accggatgat ccgcgacggc
    12061 gaccgcgtag ccgtcgcgtt gtcgggtggc aaggactccc tgacccttct tgagatcctc
    12121 attcgactgc gggatcgcgc cccggttgac ttctccgtct gcgcattcac tgtggaacaa
    12181 gggaaattcc tgagaccgat tgagccgctg ggcaattacc ttcgggagag gggaatcgct
    12241 tggacgtatt ttcaggacaa agcgtccctt cgccttgtcg aagagcaacc cggacacggc
    12301 tgcgacctgt gcagccggta tcgccggcga gcggtgtatg agatcgcccg cgggctggga
    12361 gctaacgtca ttgctttcgg tcacaccgcc gacgatttct gcgaaacgtt tctccgaaat
    12421 tcattattta ccggccgagt gagcgcgctg ccgcccgtga cgcactcccg cgaccgggag
    12481 ttccggctca tacgcccgct gctctttgta cccgaaagcc tgacgcaaca gttcaccgag
    12541 tcgctcggcg ccccggtggt gccgtgcggc tgttcacaga agacaggaac cgtgcggcga
    12601 gctctgcgag acatatttgc cagcctggaa aaggactacc caaacctgaa ggagacaatg
    12661 ctggccgcga tgggcaacct cgagccggcg cgcctgctcg acacccggta tctggatttg
    12721 gacgacgggc gcgccgaagc cgttgagcgc aatccgtttc cagtgctgcc ggagtcgtga
    12781 cgattgcctc tcggacgcca cgggatttgc atttggcgcc ctccagagtt agcctcatac
    12841 atgccgtttg gaggatacct atgttgtctc gactgcttca aagtttccgc atgagaaacc
    12901 tgcccggcct gagccgcaga gatctgtttc gaggcggagg tttgatggcc gcggccggcc
    12961 tgttcgaggg atcggtacag agcgccgccg cagacgcggg cctgcgcatc gggccggata
    13021 tctatcagtc gatcggcgtg cacccgatca tcaactgcaa gggaactttc acgatcatca
    13081 gtggctcgct ctcgctgccg gaagtcaaac aggcaatgga tgcggcctcc cggcattaca
    13141 tccaccttga tgagctgatg gatgccgtcg gcaagcggct cgccgagatt accggagccg
    13201 agttcggggt cgtcacggca ggttgcgcgg cggcgctgac acacgccacg gcggcttgca
    13261 tcgccggcgc ggacccggag aagcttcagc gacttccgta cctggaaggt ctgaagaacg
    13321 aagtcctcgt gccccgttac tcgagaaatg tgtacgacca cgcggtgcgg atgctgggcg
    13381 tgaaaatcgt cgaagccgac aacctcgatg agttcgagaa ggcgtttaac gagcggacgg
    13441 cgatggtgat ggttctggca agcccgccgg acagcggccc gttcggactg gagccgatct
    13501 gtcgaatcgc ccggctgaag agagtgccgg tcctggtgga tgcggctgcc gagaaactga
    13561 cgatcccgaa cgtccatctc cagcgcggcg cccacctggt cgcgtacagc ggcgggaagt
    13621 gcctgcgggg tccgcagtgc gcaggcgtgc ttctgggcca gaaggacctg gtcaaggcgg
    13681 cctggctgca cagcgctcct caccatgctt tcggccggtc gttgaaggtt ggcaaagagg
    13741 agattatggg tatgctggcc gcggtcgaga tgtgggtgaa gcgcgaccac gacgcggagt
    13801 ggaggcagtg ggaatcgtgg ctcgacgaaa tcgctggaca ggtgaccaag gttgacggcg
    13861 tgacgaccga cgtactccag ccgagtggcc tctcgaacta cgcgccgcaa ttgcggatca
    13921 agtgggaagg cgaaaagatc ggcatcaccg gccgcgagat ggagaaaatt ctttgggaag
    13981 aagagcccag aatcatcctg tccggcgcta gcggcgatta ccgcgacaac atggccagtt
    14041 cgatcgtaat tatgccgtac atgatgactc ccggcgatgc gcaggtcgcg ggtaagcgct
    14101 tccgggagct gctcgaaaaa ccgccgcgcg tcgaacgcaa gcgaaacacc gatccgccgg
    14161 tgcagatttc aggccagtgg gacgtccata tggaattctt ctgcggtagc ggagagcact
    14221 cgttagtctt cgaacagaag ggcagcgaac tggtaggcac gcacagcggc gacattctct
    14281 ccggcgatct gagaggcacg atggaaggaa acgaagtccg cttccgcagt tcgcacaagt
    14341 acgaggggac gcggttgggc taccgcttcg aaggcaaggt ggacggcgac atcatgcgcg
    14401 gcaccgtaga catggaagag tacggcgtcg cacagtggac cgcccaccgc cacacctacg
    14461 gccgtcctgg cggcatcgtc agaccgcaga agaacgtctg agcggcgcgg cggacgggcc
    14521 gccgcgcgag ttacttcagc tgcaaaacga ccacgtcgtc ggccgggatc gtgacgagcg
    14581 ctcccccagc cgtcagtctc cttgcatcca cagaagcggt ggcgccggaa accaccttcg
    14641 ctgatctcac ggatgacagt ggggagggaa ggttgcccgg ccgcaccaca caggtgaccg
    14701 tcttcggctc ggcgttgaaa ttcgcaagca gcaggtacgc ctcgccctgc ttgctgtaaa
    14761 ccgccgtgac aagcttctcc ccttcgattt tcaccgcgtt gttccggtag tcctcgaact
    14821 tgtagtcctc gaggttgcca aggggcttga gccgggcggc gaactcaatg gtctcctcgt
    14881 tggcgcgcca tggcatgacg gccgtcatga gtccgaggag cgcgtgttgc cgcaagatgc
    14941 gcttcggcgc gttgttatcg acagttccga tgatgatcgc ggctcgggag cgcgctcccg
    15001 cccaatccca ttcgagtgga agttcgctgg gcggaggcac ggagacggag agccgtgcgt
    15061 agccgaactc gaggccgacc accttgtcgg cgaagttctc cgtagcaaac atcggcacga
    15121 gcgtgttgtg gaggatgaac agcccgttcg gccccacccg ttcgcgagtc cattccacaa
    15181 gttcaagcaa ctcgtcgatg tcccagtggc cggtctcgga aatggcgagt gccgcgaggc
    15241 ctttctctcc cgagacgccg ttagacgtct tcccgacgtg ccgcgggtta ttgcagaaca
    15301 tggcgatgtt ccagtcgtaa taaatgccgt cgaacttgtg gttcttaagc acgcggtcga
    15361 cacagagctt catgaagtcg agccagctgg atttcaggca catgtgcgag ccatagaaag
    15421 tgttcgggcg ttggttgtcc tggtcgtccg gtttcctgcc ccattcctcg ccgtgtttct
    15481 tgaactcgtc cgtactctgg tgcagttcgt ggtttgagaa atagggtgag aggcggatgc
    15541 cgtgtcggtg aagcgtgtcg atcatggcgt ccatttttct catcacgtcc ggcggatatg
    15601 gaggataact gccatcacgc caaaagatgc cgtcttgaaa cgcatcgccg tcgttatgca
    15661 gcgtcatctc gcggataccg gaatcagcca gcttccgaat ctcgccttcc gtgatctcct
    15721 tgccgtcacg attgcggatt ccg
//
LOCUS       DATIFQ010000193         2313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1022907, whole genome shotgun sequence.
ACCESSION   DATIFQ010000193 DATIFQ010000000
VERSION     DATIFQ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022907"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VLE22_08945"
     CDS             <1..462
                     /locus_tag="VLE22_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14574.1"
                     /translation="WRGPIWMPVNALIVRALLHYYLYHGNEFTVECPTGSGRRMNLYQ
                     VAEEITRRLANIFLRDQEGRRAVYGDSHKFQKDPHWRDCLLFYEYFHGDNGAGLGASH
                     QTGWTGVIARLMHFFAVVEPAEFLEGRDRIFAEITAQAQSRQPAARVKKRG"
     gene            complement(481..1275)
                     /locus_tag="VLE22_08950"
     CDS             complement(481..1275)
                     /locus_tag="VLE22_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14575.1"
                     /translation="MKTTGFALGVLLTAIPSFAQTSPPPSPEQEEVRDEADPTQSVFF
                     SVRNEYFNLNDEAWTNALILRSDRVFFRKRRRLGGKLGILTRVDVPMVTTGVGGSTHA
                     GLGDLYGQVLYVPWLTPGFALAMGSGLTLPTATQRTLGGGKWRVAPLAVPIWFFPKRK
                     GFFLVKLQNYVSFAGDSDRLDSNHLLTTPTLVYRVRPRWWILLDTEIKTDWERDNHLS
                     FRTGFQLGRVLSPRFAIAVKPEIPWGGHREGDWALKTLFTWFRRRE"
     gene            complement(1468..>2313)
                     /locus_tag="VLE22_08955"
     CDS             complement(1468..>2313)
                     /locus_tag="VLE22_08955"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14576.1"
                     /translation="GDYVAATVRRPGSSIYDKIGTYDMTTDDVYYERGINLRHGYLAE
                     YYVDEPEYIIRVPEQLKGPGVLMEPTSVVEKAIAQAYEIQRRLRVWHPRKAAVLGAGP
                     LGLIASLLLKLRGLEVTAFGRTPRPYKNADLLDEIGVRYVNTAEMSLTEASKKYGPFD
                     FIGEGTGYSPLVFEAMDVLAKNGVLMLVSVTGGNRKAEVPADRINLGFVLGNKVVAGS
                     VNANREHFEMGAKDLSEAELQYPGWLAKLLTHPVKGLENFRELLDTLTMGKDVIKVFC
                     EVAPL"
BASE COUNT          446 a          729 c          703 g          435 t
ORIGIN      
        1 tggcgcgggc cgatctggat gcccgtcaat gctctgattg tccgcgctct gctgcattac
       61 tacctctacc acggtaacga gtttaccgtc gaatgtccca ccggctccgg gcggcgaatg
      121 aacctgtacc aagtggccga agagatcaca cgccggctgg ccaacatctt tctgcgggac
      181 caggagggcc ggcgcgcagt ctatggcgat agtcacaaat ttcagaagga tccgcactgg
      241 cgcgattgcc tgctgttcta cgagtatttt cacggcgaca acggggccgg gctcggggcc
      301 agccaccaga ccggatggac gggagtgatc gcccgtctga tgcatttctt tgccgtcgtc
      361 gagccggcag agttcctcga agggcgcgac aggatctttg ccgagataac cgcgcaagcg
      421 cagagcaggc agccggcagc cagggtcaag aagcgcggat agcttttcgg ctgaggagtc
      481 ctattctcgg cgccggaacc aggtaaaaag cgttttcagc gcccagtcac cttcgcggtg
      541 cccgccccag ggaatttccg gtttgaccgc aatcgcgaaa cgcggtgaga gcacgcggcc
      601 aagctggaac cctgtccgga acgacaagtg gttgtcccgt tcccaatcgg tcttgatttc
      661 cgtgtcgagc agaatccacc accgagggcg gacccgataa acgagagtgg gcgtggtgag
      721 cagatgattc gaatcgaggc ggtcggagtc tccggcaaac gacacatagt tttggagctt
      781 gaccaggaag aaacccttgc gcttggggaa aaaccagatg ggcaccgcga gcggagccac
      841 gcgccatttg cctcctccca gggttcgttg ggtggccgtg gggagggtca gccccgagcc
      901 catggccagg gcgaaaccgg gagtcaacca gggcacatac agaacctgcc cgtaaaggtc
      961 gccgagcccc gcgtgagttg acccaccaac tccggtggtg accatgggaa cgtcgacgcg
     1021 agtcagaatc ccgagctttc cgcccagcct ccggcgtttt cggaagaaga cacggtcgga
     1081 gcggagaatc agcgcgttgg tccacgcctc atcgttcaga ttgaaatact cattgcgaac
     1141 gctgaaaaag acactctggg ttggatcggc ctcatcgcga acctcttcct gctccgggct
     1201 gggtggcggg cttgtctggg cgaagctggg gatggcagtc agaagcactc ccaaggcgaa
     1261 tccagtggtc ttcattcgga gacacaccag caacctcctt ggtgcctggc cggttcagcc
     1321 accagaccgg gtgggcggga atcatcgctc ggaatacatt atcttgccac catcaagccg
     1381 cagcaacttt tcgcaggctg aaagatggcc tatcgcgaat cacccgcgca agctcagaac
     1441 gacccgtcgg cgggcgaggt caatggtcta cagcggcgcg acttcgcaga aaaccttgat
     1501 gacgtccttg cccatcgtca acgtgtcgag caattcccgg aagttctcga gacccttcac
     1561 cggatgcgtc agcagcttcg ccagccagcc cgggtattgc agctcggcct ccgacaagtc
     1621 cttggcgccc atctcgaagt gctcgcggtt ggcgttgacg ctgcccgcca ccactttgtt
     1681 gcccagcaca aagccgaggt tgattcggtc ggccggcact tcggccttgc ggttgccgcc
     1741 ggtcacgcta accagcatca gcacgccgtt cttggccagc acgtccatgg cctcaaacac
     1801 cagcggggag tagcccgtgc cttccccgat gaagtcgaag ggaccgtatt tcttcgacgc
     1861 ttccgtgagg ctcatctccg ccgtgttgac gtagcgcacg ccgatttcat ccagcaggtc
     1921 ggcgttcttg tagggccgcg gcgtgcgccc gaacgccgtg acctccagac cccgcagctt
     1981 gagcagcagc gaggcaatca gtcccagcgg gcctgcgccc agcaccgccg ccttgcgcgg
     2041 atgccacacc cgcagccgcc gctgaatttc gtaggcctgc gcgatcgctt tctccaccac
     2101 gctggtcggc tccatcagca cgcctggccc tttcaactgc tccggcacgc ggatgatgta
     2161 ttcgggttca tccacgtagt actccgccag atagccgtgg cgcaggttga tgccgcgctc
     2221 gtagtagacg tcgtcggtgg tcatgtcgta ggtgccgatc ttgtcgtaga tgctcgagcc
     2281 cggccggcgc accgtggcgg ccacgtagtc gcc
//
LOCUS       DATIFQ010000194         6466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_64607, whole genome shotgun sequence.
ACCESSION   DATIFQ010000194 DATIFQ010000000
VERSION     DATIFQ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64607"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..719
                     /locus_tag="VLE22_08960"
     CDS             105..719
                     /locus_tag="VLE22_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14577.1"
                     /translation="MEGVGHLLSAEQAAHFPGHGWALGWSGRRSLYFPILSVFEEVST
                     IRRRRGVYDPLDLSECDRATRSFANTFWKLVVTLRELEALARDVGRWADDDDLGRTRA
                     VFEHAPLFVDLAFVYLRRLADRFAQAIDPVLFRDRSSPRSKFRALSEYATSAEDSDLL
                     VNRERLLEVLSKSSWLERLQRLREMRNSQERIRVFTGFFALSRR"
     gene            complement(1006..1413)
                     /locus_tag="VLE22_08965"
     CDS             complement(1006..1413)
                     /locus_tag="VLE22_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_254867396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1156..1158),aa:Sec)
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSB14578.1"
                     /translation="MRKTRVKMVPEDEAVGKVKAIYDEIKVQHRIDFVPNLYKVMAPN
                     PDYLEANWSKVKAVMVNEGRLDRLTKEIIAVAVSAVNACAYULDVHTSAVRKLGLDDE
                     GVLELMAVVDLFSGFNKLMDGLQVEPDEKPWYG"
     gene            complement(1482..1994)
                     /locus_tag="VLE22_08970"
     CDS             complement(1482..1994)
                     /locus_tag="VLE22_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14579.1"
                     /translation="MRVEDFPTVLRPAPALRVYNAADPAGKMEIPVRTNGMVRMTWLR
                     IFLRVLSVGYLAAFIPWITLILLNAPILAPGSRLAPLLRFQPYNAALESMMAAIHIVW
                     AVMLWRASDDPAKHALFIDFTIWANAAHGLVMLIATPMQKGLVMTVIEGLPLFVIAAV
                     LWWLRPERSA"
     gene            2248..2694
                     /locus_tag="VLE22_08975"
     CDS             2248..2694
                     /locus_tag="VLE22_08975"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSB14580.1"
                     /translation="MTTPLALLVQVHKAIGHPVRLRLLAMLRSGPLCVCQMTVVVKLA
                     ASTVSEHLSDLRKAGLVTERKEGRWVEYRLSESALRQGILDPVWPALENDRETQADAV
                     LLKELRRVRVDELCSVELDLARIPRPKLASAVAKAAQIRATEEAQR"
     gene            2691..3749
                     /locus_tag="VLE22_08980"
     CDS             2691..3749
                     /locus_tag="VLE22_08980"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HSB14581.1"
                     /translation="MTTLASIATNGWWRATPRRGWALTATALAAAAVLYTQLQRLAAF
                     LSYDLAGLERGSHLGSAVEFFVFEVPKVLLLLTGVVFVVGVLRSFFTPERARQLLAGK
                     HEASGNVLAALFGIATPFCSCSAVPLFIGFVTAGVPLGVTFSFLISAPMVNEVALVLL
                     FGLFGWKVAALYLGTGLFIAIGAGWTIGSMKLERHVEPWVWTIQAGSAEAGAKKTWAD
                     RVTDGRAAVREIVGKVWLYVVLGIAVGAGIHGYMPTNFMASFMGREAWWAVPAAVLLG
                     VPMYSNAAGIIPVVQALLGKGAALGTVLAFMMAVIALSLPEMIILRKVLRPVLIGTFV
                     GVVAAGILVVGYLFNAVM"
     gene            3959..4210
                     /locus_tag="VLE22_08985"
     CDS             3959..4210
                     /locus_tag="VLE22_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSB14582.1"
                     /translation="MSSTQMRIEVLGPGCGRCKETFRVVQHVIEQGGLDVELVKDESI
                     ERMVALGLMATPGLVIDGKVVISGRIPKADEVRHLLGVA"
     gene            4304..5383
                     /gene="arsB"
                     /locus_tag="VLE22_08990"
     CDS             4304..5383
                     /gene="arsB"
                     /locus_tag="VLE22_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HSB14583.1"
                     /translation="MSAFERYLSAWVALCMVLGVLIGKFVPRLVGAVRDMEFGTGSHI
                     NIPIAILIWLMIYPMMLKLDFGSIVGVGRKPKGLVITLVVNWLVKPFSMAFLGWLFFK
                     HLFLPLIGQELANQYIAGVIILAAAPCTAMVFVWSYLANGDPAYTLVQVTLNDLFMLV
                     LFAPVVKFLVSGTSGLEVPFQVLIYSVLIFIVIPLALGSLSRTLLLRRKGVDWFTNRF
                     LAFFQPVTILALLATLVLIFAFQAENITTRWFHVILIAIPILIQVYFNASLTYGLMKL
                     FHVNYEVAAPGALIGASNFFELAVATAIALYGPGSGAALATVVGVLVEVPVMLSVCRM
                     CITTRHWFRTEKSPVPAAARELTVD"
     gene            complement(5764..5976)
                     /locus_tag="VLE22_08995"
     CDS             complement(5764..5976)
                     /locus_tag="VLE22_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14584.1"
                     /translation="MATLLKEMFEKPADTAALEAALDRSLVPDEAELQKIMRYSSYLS
                     RQEERYVEMLLKLQEVRREREREGHI"
     gene            6129..6359
                     /locus_tag="VLE22_09000"
     CDS             6129..6359
                     /locus_tag="VLE22_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14585.1"
                     /translation="MSWRRSGFSVHNRVYVPAGDSQGLEALVCYMMRSPMDPKGMISA
                     AEGAMREARESRESRPGLTVASRKRKLPRFTA"
BASE COUNT         1130 a         2052 c         1957 g         1327 t
ORIGIN      
        1 ggccgagatt ccggcccgtt gaagaagcgc agaaccaatc ccctagccct gatggacaga
       61 ctactcgttg tcgcggttgc ctgaccgttc gctaggatcg gccgatggaa ggggtggggc
      121 atctcctgtc cgccgagcag gccgctcact tccctgggca tggttgggct ctcgggtgga
      181 gcggacggcg atcactgtac ttcccgatcc tgtctgtttt cgaggaggtg tcaacgattc
      241 gcagaagacg gggcgtttac gatccgctgg atctctcgga gtgcgaccgc gcgacaagga
      301 gcttcgccaa caccttttgg aagctcgtgg tcaccctccg tgaattggag gctctcgcgc
      361 gggacgtggg acgctgggcg gacgacgacg atctcgggcg gactcgagca gtctttgagc
      421 acgcgccact ctttgtcgat ctcgccttcg tgtatctccg tcggttagcc gatcgattcg
      481 cgcaggcgat agatccggtt ctgttccgtg accgatcctc gcctcgctcg aaattccggg
      541 cgctgagcga gtacgctacg agcgctgaag atagcgacct actcgtgaac agggagcgcc
      601 tgttggaagt cctctccaag agttcctggc tggagcgact ccagagattg agggagatgc
      661 gaaactccca ggagagaatt cgcgtcttta cgggtttttt cgctctgagc cgacggtgaa
      721 ggagttctca aaacccaaag gtcgaaggtt caaatccttc cccccgcaac catgaaaaca
      781 aaggagttgg gcgagttgcc caattccttt cccgtttgac ttaccggatt cttactgggt
      841 tcctccgctc aagggctcag cgctatgggc tcatcaggtt cggctcgccg cgaacgggca
      901 cgcagatgag atgatgcctc tcccgaccga gggacgacgc acggcgctcc atcgatccag
      961 aaatgttcaa gagtcggatg cgcccggcac ccagtcgatc tagcctcacc cataccaagg
     1021 cttctcatca ggttcgacct gtaaaccgtc catcagcttg ttgaagccgc taaacagatc
     1081 gacgaccgcc atcaactcca gcaccccttc atcgtccaga cccagcttcc gcaccgcgga
     1141 agtgtggacg tccagtcagt aggcgcaggc gttcaccgcg gacacggcaa ccgcaataat
     1201 ctccttcgtc aagcggtcca gcctaccctc gttgaccatc accgctttga ctttgctcca
     1261 gttcgcctcc agatagtcgg gattcggtgc catcaccttg tatagattcg gcacgaagtc
     1321 gatccggtgc tgaaccttga tctcgtcgta aatggccttc acctttccga cggcctcatc
     1381 ctccgggacc atcttcactc ttgtctttct catcgcgccc tatcctttct ccgggacgac
     1441 tgcaacgcac gagattgctt ggaacacgcc gggcggcctg atcaggccga tcgctcaggc
     1501 cgcagccacc acagcactgc ggctatcacg aacagcggca gaccttcgat gaccgtcatg
     1561 acgaggccct tctgcatggg tgtcgctatg agcatcacga gaccatgagc ggcgttcgcc
     1621 cagatggtga aatcgatgaa gagagcgtgc tttgcagggt cgtctgaagc acgccacagc
     1681 atgaccgccc acacgatgtg gatcgcggcc atcatgcttt cgagggcggc attgtagggc
     1741 tggaatctga gcagcggcgc aagccgactt ccgggcgcga ggatgggcgc gttcaggaga
     1801 atgagtgtga tccacggaat gaacgctgcg aggtatccaa cggacaggac ccgcaggaaa
     1861 atacgcagcc aggtcatcct caccatgccg ttggttctga ccgggatttc cattttgccc
     1921 gccggatcgg ccgcgttata cacccgaagc gcgggagccg gccgaaggac cgtcgggaaa
     1981 tcctccaccc gcaaccatga ttcccaagga gttaggcgag gccgcctgac tccttttctt
     2041 tcaggtcgag cgaaagtgga gattcgctgg agcacatttc cgtcatttca aacttccacc
     2101 aacagcggct tcacaacgca agagcttcgt atccgggcgt gccatccggg ccgagaaatt
     2161 cgcctccggg gcgcctcgcc gggatgggca agacgcatct tgacaagtcg cggcgggtgt
     2221 ctctaagata tttcgtgaga ttacgaagtg acgacaccgc ttgctctcct cgtccaagtc
     2281 cacaaagcca tcggccaccc ggtacgcctg cggctgctcg cgatgctccg cagcggaccc
     2341 ctctgcgtct gccagatgac cgttgtcgtg aagcttgcgg cgtccaccgt ttccgaacac
     2401 ctgtcggacc tccgcaaggc cggtctcgtc accgagcgca aggaaggccg gtgggtcgag
     2461 taccggctct ccgaatccgc cctgcggcag ggcatcctcg atccggtctg gccggcactg
     2521 gagaacgacc gtgaaacgca ggcggacgcg gtgctcctga aggaactgcg gcgcgtgagg
     2581 gtcgatgagc tctgcagcgt cgaactcgat ctcgcgcgga tcccacgccc gaagctcgcg
     2641 tccgcggtgg cgaaggcggc gcagatccgc gccaccgaag aagcacagcg atgacaacac
     2701 tcgcctccat cgcgacgaac gggtggtggc gcgcgacgcc gaggcgcggc tgggcgctaa
     2761 ccgcgaccgc cctagccgcc gccgctgtgc tctacacgca gcttcagcgg ctcgccgcat
     2821 tcctctcgta cgacctggcc ggcctcgagc gcggcagcca cctcggatct gcggtcgagt
     2881 tcttcgtgtt cgaggtgccg aaggttctcc tgctgctcac cggcgtcgtc ttcgtcgttg
     2941 gggtgctgcg aagcttcttc acgccggagc gcgcgcggca gctcctcgct ggaaagcatg
     3001 aagcatccgg caacgtgctc gctgcgctct tcggcattgc gacgccgttc tgctcgtgct
     3061 cggcggtgcc actcttcatc gggttcgtta ctgcgggggt gccgctgggg gtgacgttct
     3121 cgttcctgat ctccgcgccg atggtcaacg aggtggcgct cgttctcctc ttcggcctgt
     3181 tcggctggaa ggtcgcagcc ctctacctcg gcacgggact cttcatcgcg atcggggccg
     3241 gctggacgat cggaagcatg aagctcgaac ggcacgtcga gccgtgggtg tggacgatcc
     3301 aagccggcag cgcagaagcc ggcgcgaaga agacctgggc cgaccgggtg acggacggcc
     3361 gcgcggccgt ccgcgagatc gtcggcaagg tatggctcta cgtggtgctc gggatcgcgg
     3421 tcggggccgg cattcacggc tacatgccga ccaacttcat ggcttccttc atggggcgag
     3481 aggcgtggtg ggcggtcccc gccgcggtcc tcctcggtgt ccccatgtac tcgaacgcgg
     3541 ccggcatcat cccggtcgtc caggcgctcc tcggcaaggg cgcggcgctc ggaaccgtcc
     3601 tcgcgttcat gatggcggtg atcgcgctgt cgctgcccga gatgatcatc ctccggaaag
     3661 tgcttcgccc ggtcctgatc ggcacctttg tcggcgtcgt ggcggcgggc attctcgtgg
     3721 tcggatacct cttcaacgcg gtgatgtgat gcacgctcgc gacacggcaa cacggcggct
     3781 cgcgtggggg ttcgtgagca gcacggccgt cctgggccgc gcgaccgcca acgccgaaga
     3841 caggctcgca atcgagcacg acaccttcca gatcgagtcc gcggatgccc cctgcgtcgc
     3901 agctcccagc tgtgggtcca ccgtttcgct cccttccacc acctgacagg agaacgcaat
     3961 gtcatcgaca cagatgagaa tcgaagtgct cggccccgga tgcgggcgct gcaaggagac
     4021 cttccgtgtg gtgcagcacg tcatcgagca gggaggcctc gacgtggagc tcgtcaaaga
     4081 cgagtccatc gagcggatgg ttgcgctcgg cctgatggcg acgcccggtt tggtgattga
     4141 cggcaaggtc gtcatttccg ggcgcattcc gaaggctgac gaagtccggc acctgctcgg
     4201 tgtcgcatga agcgagcttg ggccatcaga ccgggctgaa tcgaatgtcg attagcacga
     4261 tcaaaacgat cgaggttccc gaacgagctc acagtcgcct cagatgagcg ctttcgagcg
     4321 ctacctcagc gcctgggttg ccctctgcat ggtcctcggt gtcctcatcg gcaagttcgt
     4381 gccgcgcctc gtcggcgctg tgcgtgacat ggagttcggc accggcagcc acatcaacat
     4441 cccgatcgcc attctgatct ggctgatgat ctatccgatg atgctgaagc tcgattttgg
     4501 atcgatcgtc ggcgtcgggc ggaagccgaa ggggctcgtt atcacgcttg tcgtcaactg
     4561 gctggtgaag ccgttctcca tggcgttcct cggctggctc tttttcaaac acctctttct
     4621 gccgctcatc ggacaggagc ttgcaaacca gtacatcgcc ggggtcatca tcctcgctgc
     4681 agccccctgc acggcgatgg tgttcgtctg gtcgtacctg gcgaatggcg atccggccta
     4741 cacgctcgtt caggtgacgc tcaatgacct gttcatgctg gtgctgttcg cccccgttgt
     4801 gaagttcctc gtctccggca cctccggtct cgaggtgcca ttccaggtgc tcatctactc
     4861 ggtgctgatc ttcatcgtca ttcctctagc gctcgggtcg ctgagccgca cgctactcct
     4921 gcggcgcaag ggggtggatt ggttcacgaa ccggtttctc gccttcttcc agccggtgac
     4981 gatcctggcg ctcctcgcga cgctcgtgct gattttcgcg ttccaggcgg agaacatcac
     5041 cacgcggtgg ttccacgtga tcctgatcgc catcccgatc ctcattcagg tctacttcaa
     5101 cgcgtccttg acctacgggc tgatgaaact ctttcacgtc aattacgagg ttgctgcgcc
     5161 cggtgccctc atcggcgcgt cgaacttctt cgagctcgcc gtcgccaccg cgatcgcgct
     5221 ttacggcccc ggctccggtg cagcactcgc gactgtcgtc ggcgtcctcg tcgaggttcc
     5281 ggtgatgctc tctgtctgcc ggatgtgcat caccaccagg cactggtttc gcactgagaa
     5341 gtcgcccgtt cccgcggctg cgcgggagtt gacggtcgat taagaagttc cttgcttgca
     5401 gagaccgatt ggggtgctct cgagaacctc tcctggcgac gtctaacctg tccggatcga
     5461 ggggattcgt tcgcaaagaa ttcgaaccgc cggagagatc ccgttccagg cggccctgtt
     5521 tgaggcggtc atccccgcgt accggcgaat cgccctgatc gaggcgcctg cgcagcctgg
     5581 ggctcagcct atccgcgatc ggccgtcacc tgggcgtcac ggacaaaacc gtcgccaagg
     5641 cgctccggtc ggttggatct ctcggcgaaa gctaggcctc gaccgatgtc ggcccgccga
     5701 cggcgatttc ggcaatcgat tccggtgggt tcgtttcgca gaaatagtct tctttggtgg
     5761 tccctagatg tgaccctccc tctcccgctc acgccgtact tcctgcagct tcagcagcat
     5821 ctcgacgtat cgctcttcct ggcgtgacag atagctcgaa taccgcatga tcttttggag
     5881 ctcagcttcg tccgggacca gcgaccggtc cagtgccgcc tccagcgcgg ccgtgtccgc
     5941 cggcttctcg aacatttcct tcaggagcgt cgccatttca gaaaggtaac ccgggagggg
     6001 atcgtcgaga cattgaaaag gtcaccccgt gatttggcag tccggagttt tggttctctt
     6061 tttttattga tcgccgggga agatttgttc tccagaccga cgcaggatgg gaagagctac
     6121 gaccgctgtt gtcgtggagg cgaagcggct tctccgtcca caaccgggtc tatgtgcccg
     6181 ccggggatag ccagggtctc gaggcgctgg tctgctacat gatgcgctcc ccgatggatc
     6241 cgaaaggcat gataagcgcc gccgagggag ccatgcgaga ggcgcgcgaa agccgcgaaa
     6301 gccggccggg cttgacggtc gcctccagga agaggaaact tccccgtttt accgcctgag
     6361 acccgggagc gaaggtgacc atggacgacg acggctatcc ctcaccccaa ggcctctacg
     6421 atccccgcta cgagcacgac gcctgcggat tcggcttcgt cgtcga
//
LOCUS       DATIFQ010000195        12560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1406034, whole genome shotgun sequence.
ACCESSION   DATIFQ010000195 DATIFQ010000000
VERSION     DATIFQ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12560
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406034"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VLE22_09005"
     CDS             <1..246
                     /locus_tag="VLE22_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14586.1"
                     /translation="LEFPAGESRESLGLTGTETFHIEGVKEAVTGGRRATVRALSADG
                     IERTFQAVVRVDTPREADYYRYGGILPYVLRQLAAGA"
     gene            243..2441
                     /locus_tag="VLE22_09010"
     CDS             243..2441
                     /locus_tag="VLE22_09010"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSB14587.1"
                     /translation="MRRPVRTCIALAALASVAFGQAKSWRGWDTYHFIMWSTGAPRDW
                     GTWINRLREIGFTAEELTAGRDAGPFTMNNFGFYVENLVSELAYLHSRKPLYDADWQG
                     YTTTRDKKYLVRNPCFHDPDYWPTAKARVQSLVQPYAGRSPLLHDLRDELSLGSFASP
                     MDYCFGPHTLREFRDWLKSEYGTLEALNAEWDTQFPSWDSVMPMTTYEVKDRERAALA
                     AGRLENYAPWSDHRAFMDLTFARTLDRFRGYIREVDDATPVGVEGTQMPSAWGGYDLW
                     RMSQAIDWVEAYDIASSREMFRSFLPEGTPILCTLFGSDFPRLKQKVWWRLLHGDRGL
                     LVWDDEESRSVLKDQDGMPVTDRGRGLAEIIAGVQPVAPLLLSLKRVDDRIAVHYSQA
                     SIRAHWMFDSREDRDTWPRRFSSYEATWSRIARVRDSFVRVIEDLGLQYNFVSYEQLE
                     NGELLRGGYRVLLLPQSVAMSEKECRAVEEFVRRGGTVIVDNMTATMDARCRRLPRGQ
                     LDGLFGIERTSVGWSASPLGGHLTQPDAAVVPLEGFEPDIRVTSGAARETTATNVPVV
                     IANRTGMGRTVYLNLDMHQYGKLRLTPPKGQAYTELFKQLLLESRIEAPVKVVDSVGG
                     RPVPGVEVWRYSGNGGQYVAVMRSPEFNASSLAETGYPDNAELEKTVRVRITVRGTKK
                     LTDVRGGASVSGNSIEMDLEPWSPAILKTEAGSVERGPRSGRPRPVERER"
     gene            2538..2675
                     /locus_tag="VLE22_09015"
     CDS             2538..2675
                     /locus_tag="VLE22_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14588.1"
                     /translation="MKDFHELKVWQEAHQLPRDYEESSERTIEVKRMLAALIQKLNAD
                     R"
     gene            complement(2715..3530)
                     /locus_tag="VLE22_09020"
     CDS             complement(2715..3530)
                     /locus_tag="VLE22_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HSB14589.1"
                     /translation="MPSSAVVYARKAQYLLLELALPGRSKVTAGVLLLDPSDDRLYLK
                     LRPDWDRIADPDDVELLQLLDDDLLRHSREMGAETFLRSLEDSFSNALRITDRESIVI
                     ADPEKALNRAYRRHVEGGGESGRPSLPIYSLRAAAGKFGEDLEVEPEGFAPVPEGMRP
                     ESDLFVAHIIGRSMEPAIPDGCLCVFRPYRGGSREGRWMLVWNRGASAGGGEFTIKRY
                     TSTKTHREDGSWEHTGIRLEPLNPEYDDLVLNPDPDRYATVAEFLRVLPYEDL"
     gene            complement(3565..4602)
                     /locus_tag="VLE22_09025"
     CDS             complement(3565..4602)
                     /locus_tag="VLE22_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB14590.1"
                     /translation="MLGRIVLAGCLALPLAAQEAKPPSAAAPVSGQHAPPAPIKIEVN
                     EVVVPVTVTDQNDRFVSDLEQKDFRIFDEGKLQKVLYFSRERSQPVVVGFLLDLSNAS
                     RLHWAKFQDAALELVFNLLPGDPRFSGYLISFGNDAELQVNTTSDPEKIVEKIRKLKP
                     GGGAAFFDAIYMACTRRSLVEGEPIEPRRVLIVIGDGHDSASKHSIQEVLELAQRNLI
                     TIYGMSTVAFGFNSEGEHNLKRLAEDTGGRVEYPLQGLYSDVSGFLSTPSDEGNYALK
                     VGTGQYAAEIAKGIFESVAALAGEVTTQYILRYVPDTTDTARTFRNIRVDVRLPNVKV
                     RARKGYFPNTP"
     gene            complement(4707..5741)
                     /locus_tag="VLE22_09030"
     CDS             complement(4707..5741)
                     /locus_tag="VLE22_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HSB14591.1"
                     /translation="MSKTVVTFGEIMLRLAPPGFERFLQSPQFVATFGGGEANVAVAL
                     GGFGVPARYVTVLPPNNPISDAFIGEMRRFGVDPSSIVRGKGRFGIYFVEAGANQRPS
                     KVVYDRENSAIALAKLGDIDWDKAFDGAGWFHVTGITPAISQSAADLALEAVQKARAK
                     GLTVSCDLNYRKNLWKYGKTAKEVMTELVKFVDIGIANEEDCQMALGVQAEADVHAGK
                     LEVSHFRDLTTRVMTQFSNLKMLAITLRESKSASHNGWSACLNDGTGFLVSRHYDITH
                     IVDRVGGGDCFAGGLIYGLLNLPSHQDAIEFAVAASCLKHSIPGDFSRFTADEVNSLL
                     KSGGSGRVQR"
     gene            complement(5760..6896)
                     /locus_tag="VLE22_09035"
     CDS             complement(5760..6896)
                     /locus_tag="VLE22_09035"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSB14592.1"
                     /translation="MRKPAVFVLILFALSAILPAVLSAQRKKGKGEKEEVTQTLEVPK
                     DPPATIQADTSRIFFDVSPLTPKGLLSQQVRDALKALQRTARRGAIVKLRAFVAGTGD
                     MRRVQTIVSESFTERRLPLPVLSVVQVGALPMEGAQVVIEATGVARKTENPHGIAFIS
                     GQMAASDEMLLDVAPLARKSIASVADAVKAAGAQPVDILRATCFLSSLQDVNAVRGIA
                     SAQFPQAEWNFVQILRAPARAVVECEAVARLRAPAGAPLRLLNPPGLPQSPNYSQIAL
                     VGAPKLLLSGSRLAFRFQPDDARLAFQRLGRDLEQAGGSLRHVAMSSIYPLSDAVGDL
                     VRKVRFEFYDKDNPPASTMLPFEGLPGLDASFAVEVVAVLPTSQ"
     gene            6987..8468
                     /locus_tag="VLE22_09040"
     CDS             6987..8468
                     /locus_tag="VLE22_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HSB14593.1"
                     /translation="MLPAPVLDPESEVPLYRQLHEHVRELIGAGRLKPGDRLPSTRDL
                     AGHLGLNRTTVSAAYELLETEGLIRGHVGRGSFVTGSVPSESSGLDWAEVLESWDRGS
                     FPQPAAAPEEMISFATSRPADHLFPIDEFRATCREVVAAEGAAAILQLGSPSGYGPLR
                     DYLIDEGREEGVAGPGDDVVVTNGCQQAMDLIQRVLLRPGDTVLLEDPVYPGAKGVFA
                     RAGTRVVGVPVGAQGMDLEHLERAIAREKPRLLVVTPNFQNPTGATLPLAARRTILEM
                     VREARVVLVENDIYGALRYEGEPLPTLKQLDDTGDTVLLRSYSKLAFPGLRVGWVTGP
                     HALSGRLAEAKQFTDLHSDQLSQAVLLRFAESGRLAAHRARIVAAGAERLSAVIEACG
                     RYLPDGTRLTRPQGGMNLWVRLPQPLDAGELLARAHQENVSYLPGKYFEVSRHDPGGL
                     RLSFAGLTPEKIRAGVAILGGIFSNELERARASRHFDPAPAMV"
     gene            8480..9364
                     /gene="pdxS"
                     /locus_tag="VLE22_09045"
     CDS             8480..9364
                     /gene="pdxS"
                     /locus_tag="VLE22_09045"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HSB14594.1"
                     /translation="MFLDDTFRVKVGLAEMLKGGVIMDVTNAEQALIAEKAGAAAVMA
                     LERVPADIRKEGGVARMAPIRKIREIMEAVSIPVMAKARIGHFMEGRVLESLEVDYID
                     ESEVLTPADEEHHIDKRQFKVPFVCGARNLGEALRRIAEGAAMIRTKGEAGSGNIVEA
                     VRHMRTIQKEMKQLTVLGKEELVAESKKLQAPLELVEYVAEHGKLPVPNFSAGGIATP
                     ADAALVLALGAEAVFVGSGIFKSDDPDARARAIVKATTYWKDPAKLLEASEDLGMGMP
                     GLDVSKLQESELLQTRGW"
     gene            9434..10012
                     /gene="pdxT"
                     /locus_tag="VLE22_09050"
     CDS             9434..10012
                     /gene="pdxT"
                     /locus_tag="VLE22_09050"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HSB14595.1"
                     /translation="MKKVGVLALQGDFEAHQKALDRAGAEAIEVRTAAELEGVDGLVI
                     PGGESTTMLKLLREEKLFEPLRRFGERRPIFGTCAGTILLATEVSHPTQESLGLMDIS
                     VERNGYGRQLDSHVAVGCPEDEFLRRTGEGDLEMVFIRAPVIRRAGGDVKVLVEHRGA
                     AVLVEQGRHLAATFHPELGADERVHRLFLAKL"
     gene            10023..10424
                     /locus_tag="VLE22_09055"
     CDS             10023..10424
                     /locus_tag="VLE22_09055"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HSB14596.1"
                     /translation="MRVLFVCLGNSCRSQMAEGFARAHGRGVWGADSAGLMPATIVAP
                     LTRRVMEEKNISLEEHFPKGLDEIRLSDYGLVVNMSGYEMPGIVTAPVRTWDVRDPIG
                     QSEKVYREVRDEIERLVLGLLSEVRARTGAG"
     gene            10473..11435
                     /locus_tag="VLE22_09060"
     CDS             10473..11435
                     /locus_tag="VLE22_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSB14597.1"
                     /translation="MAGDAFQAYLLAQIEGREERFPLETGMVCRIGRGEENSVVLGDN
                     LVSRRHALLQCTQGGEYFLVDLGSRNGTLLNGRRVTVPVTLKTGDRISIGSYEIEFQG
                     PPAIAEDKASFGETMLAVSHRLITVLVADIRDFTGLSRRIGEARLSEMMSVFFGECGR
                     VLAEHGAWGQKYIGDAVMAFWVHQREIPDRRELAGVFASLAEIFDTAAGLQKRVGLDA
                     AIRIGAGVNTGLASVANIGSVAASDYTAVSEAVNLAFRLETATKEVGCDVVLGQETVA
                     RLAQFADVSHLFDMRTVTLKGYENPMPAYAGRAESVAELRRILA"
     gene            11507..11593
                     /locus_tag="VLE22_09065"
     tRNA            11507..11593
                     /locus_tag="VLE22_09065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11541..11543,aa:Leu,seq:cag)
BASE COUNT         2334 a         3563 c         4415 g         2248 t
ORIGIN      
        1 cttgaattcc cggcgggcga gagccgggag tcgctcgggc tgacgggcac ggagacattc
       61 cacatcgaag gcgtgaaaga agcagtgacc ggcgggcggc gagcaacggt gcgcgctctg
      121 tccgcggacg gcattgagag aaccttccag gcggttgtgc gtgtggatac gccacgcgaa
      181 gcggactact accgttatgg cgggattctg ccgtatgtgc tgcggcaact ggcggccggg
      241 gcgtgagacg gcccgttcgg acgtgcatcg cactggcggc gctcgcgtcc gtggcgttcg
      301 gccaggcgaa gtcctggcgc ggctgggaca cctaccactt catcatgtgg tccacgggcg
      361 ccccgaggga ctggggcacg tggatcaacc gcctgcgtga aatcggcttc actgccgagg
      421 aactaacggc gggccgtgac gccggtccgt tcacgatgaa caatttcgga ttttacgtcg
      481 agaacctggt ttccgaactg gcctaccttc atagccggaa gccgttgtac gacgcggact
      541 ggcaggggta tacaacgacc cgcgacaaga agtatctggt gcgaaatccc tgctttcacg
      601 acccggacta ctggcccacg gccaaggcgc gcgtgcaatc gctggtgcag ccgtatgccg
      661 gccggtcgcc gctgctgcac gaccttcggg acgagctctc gctggggtcg ttcgcctcgc
      721 cgatggacta ctgcttcggg ccgcacacgt tgcgcgagtt tcgcgactgg ttgaagtcgg
      781 agtacgggac gctcgaagcc ttgaacgcgg aatgggacac acagttcccg tcgtgggatt
      841 cggtgatgcc gatgacgacg tacgaggtga aagaccggga gcgggcggcg ttggcggctg
      901 gcaggctcga gaactatgct ccatggtcgg atcatcgcgc gttcatggat cttaccttcg
      961 cgcgaacact cgaccggttc cgggggtata tccgggaggt tgacgatgcg acgcccgtag
     1021 gtgtggaagg cacgcagatg ccgagcgcgt ggggcgggta tgacctgtgg cggatgtcgc
     1081 aagcgatcga ctgggtggaa gcctacgaca ttgcttcgag ccgcgagatg tttcgctcct
     1141 tccttccgga gggaacgcca atcctctgca cgctgttcgg gagcgacttc ccgcggctca
     1201 aacagaaggt gtggtggcga ctgctgcacg gcgaccgcgg cctgctggta tgggacgacg
     1261 aagagagccg gagcgtccta aaggaccagg atgggatgcc ggtgaccgac cggggcaggg
     1321 gactggccga gattatcgcc ggggtgcagc cggtggcgcc cctcctgctc agcctgaagc
     1381 gcgtggacga caggatcgcg gtccactatt cgcaggcgag cattcgcgcg cactggatgt
     1441 tcgattcccg cgaggacaga gacacatggc cgcggcggtt ctcttcgtac gaggcgacgt
     1501 ggtcacggat cgcccgggtg cgggacagct tcgtccgggt gatcgaggac ctgggactcc
     1561 agtacaactt cgtttcatat gaacagttgg agaacggcga actgctgcgg ggcgggtaca
     1621 gagttctgct gctgccgcag tcggtggcga tgtctgagaa ggagtgccgg gcggtcgagg
     1681 agttcgtccg gcggggcggc acggtgatcg tggacaacat gacggcgaca atggacgcgc
     1741 gctgccggcg gctgccgaga gggcaactcg acgggctgtt tgggatcgag cggacgagtg
     1801 tgggctggag cgcgtcgccc ctgggcgggc atttgacgca gccggatgcg gcagtggtgc
     1861 cgctggaagg attcgagccg gacatccgtg tgacctcggg tgcggcgcgg gagacgacgg
     1921 ccacgaacgt accggtcgtg atcgcgaacc gcactggcat ggggcggaca gtgtatctca
     1981 acctcgacat gcaccagtac ggcaagttga ggctgacgcc gccgaagggc caggcgtaca
     2041 cggaattgtt caagcagttg ctgctggagt cgaggattga agcgccggtg aaggtcgtgg
     2101 attcggtcgg cggcagaccc gtgccgggcg ttgaggtgtg gcgatactcg gggaacggtg
     2161 ggcagtacgt ggcggtgatg cggagcccgg aattcaacgc gtcgtccctg gcggagaccg
     2221 ggtatccgga caacgcggag ctggagaaga ccgtccgggt gcggatcacg gttcggggaa
     2281 cgaagaagct gacggatgtg cggggtggcg ccagcgtgag cggcaattcg atcgagatgg
     2341 atctggagcc gtggagcccg gcgattctga aaacggaggc tggttccgtc gagcgtgggc
     2401 ctaggagcgg gcggccgcgg ccggtggagc gggaaaggta gcaagctctc aggctgtcag
     2461 cggtcagctt tcagcgatca gcacttagcg aagatcatcg ggcgggcagc cgatggaaac
     2521 ccaaggggac atgcaccgtg aaagatttcc acgaactgaa agtctggcaa gaggcgcacc
     2581 aattgccgcg ggactatgag gagtcgtccg aacgcaccat cgaggtaaaa cggatgcttg
     2641 ccgcactcat tcaaaagctg aacgctgacc gctgatagct gatggctgat agctgatagc
     2701 tgaccgttcc caactcagag gtcctcgtac ggcaggacgc ggaggaattc ggcgaccgtc
     2761 gcgtagcggt ccgggtcggg attgaggacc aggtcgtcgt attcggggtt gaggggctcg
     2821 agacgtatgc cggtgtgttc ccaggaaccg tcctcgcggt gcgttttggt actggtgtag
     2881 cgcttgatgg tgaactcacc gcctccggcc gaagcgccgc gattccagac gagcatccat
     2941 cttccttcgc gcgagccacc gcggtagggg cggaacacac acaggcagcc gtccgggatc
     3001 gcaggctcca tcgagcgccc gatgatatgc gcgacgaaca ggtcactttc cggccgcatt
     3061 ccttccggaa ccggcgcgaa gccttcgggc tccacttcca ggtcctcgcc gaacttgcca
     3121 gctgccgctc ggagcgaata gatcggaagg ctgggccggc ccgactctcc gccgccctcg
     3181 acgtgccttc gatacgcgcg attcagcgcc ttttcggggt ccgcaatgac gatgctttcg
     3241 cggtcggtga tccggagagc gttcgagaag gaatcctcca gggacctcaa gaacgtttcg
     3301 gcgcccattt cccggctgtg tcggagaagg tcgtcgtcga ggagctgcag caactcgacg
     3361 tcgtcgggat cggcgatcct gtcccagtcc gggcgaagct tgaggtagag tcgatcatca
     3421 gaagggtcaa gcaggagcac gccggcggtt accttcgacc ggccgggcaa ggccagctca
     3481 agcagcaggt attgcgcctt ccgtgcgtac acgaccgctg atgagggcat tcacaagata
     3541 ttatcggatg gaaagcgcaa aaggttaggg tgtattcgga aaatacccct tgcgggcccg
     3601 gactttcacg tttggcagcc tgacatcgac gcggatgttg cggaaggttc gcgcggtgtc
     3661 ggttgtgtca ggcacgtagc gcaggatgta ctgagtggtt acctcaccgg cgagggcggc
     3721 gacggattcg aagatgccct tggcgatctc cgcggcgtac tggcctgtgc caacctttag
     3781 tgcatagttg ccctcgtcgg acggagtgga gaggaacccg gaaacgtcgg agtacagacc
     3841 ctgaagcggg tactcgacgc gcccgccggt atcttccgcg agacgcttga ggttgtgctc
     3901 gccttcgctg ttgaagccga aggcgacggt actcattccg tagatggtga tcaggtttcg
     3961 ctgcgcgagc tcgagcactt cctggatgct gtgtttgcta gcggagtcgt ggccgtcgcc
     4021 gatgacgatg agaacgcggc ggggttcgat cggctcgcct tctaccaggc ttcggcgagt
     4081 gcacgccatg tagatcgcgt cgaagaacgc cgcgccgccg ccgggcttca gcttgcgaat
     4141 cttctccacg atcttctcgg ggtcgctggt ggtgttcacc tgtagctcgg cgtcgttgcc
     4201 gaaagagatc aggtagccgc tgaagcgcgg gtctcccggc aggaggttga agacgagctc
     4261 gagcgccgca tcctgaaact tcgcccaatg gagccgggaa gcattgctca ggtcgagcag
     4321 gaagccgacg acgacgggct ggctgcgctc gcgactgaaa tagaggacct tctgtagctt
     4381 gccctcgtcg aagatgcgaa agtccttctg ctcgaggtcg gaaacgaaac ggtcattctg
     4441 atcggtgacc gtgacaggca cgaccacctc gttgacttca atcttgatcg gcgcaggcgg
     4501 agcatgctgg ccggagacag gcgccgccgc ggacggcggc ttcgcctctt gtgccgcgag
     4561 aggcaacgcg agacaccctg caagtacgat gcgccccagc atcctaatac ccaagtatac
     4621 cgtgtgatgg tgcggaccgg agccactgtc cacgctcgtc agcagcttgg acggtggttc
     4681 cagccccaag gtttccggtt tcgagcctac cgctgcaccc ggcccgagcc gccgctcttg
     4741 agcagcgaat tcacttcgtc cgcggtgaac cggctgaagt cacccggaat cgagtgcttc
     4801 aggcacgagg cggcgacggc gaactcaatg gcatcctggt gcgaaggcag gttgagcagc
     4861 ccgtagatga ggccgccggc gaaacagtcg ccgcctccga cccgatccac gatatgggtg
     4921 atgtcgtaat ggcgactgac aaggaagccg gttccgtcgt tgaggcacgc cgaccagccg
     4981 ttgtgcgatg cgctcttgga ttcgcgcagc gtgatggcga gcatcttcag gttcgagaac
     5041 tgggtcatca cacgggtggt gaggtcgcgg aagtgggaga cctccagctt gccggcgtgg
     5101 acgtcagcct cggcctggac gccaagcgcc atctggcagt cctcttcgtt ggcgatgcca
     5161 atgtcgacga acttaacgag ctcggtcatg acctccttgg ccgtcttgcc gtacttccag
     5221 aggttcttgc ggtagttgag gtcgcaggag acggtgagcc ccttcgcccg cgccttctgg
     5281 acggcctcga gagcaagatc ggccgcgctc tggctgattg cgggggtgat gccggtcacg
     5341 tggaaccagc cggcgccatc gaaagccttg tcccagtcga tgtctccgag tttggcgagg
     5401 gcgatggcgc tgttctcgcg atcgtagacg accttggaag gccgctggtt tgcgcctgcc
     5461 tccacgaaat agatgccgaa gcggcctttg ccgcggacga tcgaggaggg gtcgacacca
     5521 aagcggcgca tctcgccgat gaaggcatcc gagatcgggt tgtttggggg caggacggtg
     5581 acgtagcggg cgggaacgcc gaagccgccg agggcaacgg ccacgttcgc ctcgccgccg
     5641 ccgaaggtgg cgacgaactg cggggattga agaaagcgtt cgaagcccgg aggggccaaa
     5701 cgcagcatga tttcaccgaa ggtaacgacg gtcttggaca taacggttaa gaaaatctcc
     5761 tattgggaag tcggcaaaac ggcgacaacc tccacggcga agctggcgtc gaggccgggc
     5821 aggccctcga aaggcagcat cgtgctggcg gggggattgt ccttgtcata aaactcgaag
     5881 cgcaccttcc gcaccaggtc tccaaccgcg tcggagaggg gatagatgct cgacatggcg
     5941 acatggcgga gcgaaccgcc ggcctgttcc aggtcgcggc cgagccgctg gaaagcgagc
     6001 ctggcgtcgt ccggctggaa acggaacgcc agcctgctcc cggacagcag gagtttcggc
     6061 gcccccacca gcgcaatttg cgagtagttc ggagattgcg gaaggccggg aggattcagc
     6121 aggcgaagcg gtgcgccggc gggcgcgcga aggcgcgcga cggcctcgca ctcgacgacc
     6181 gccctggcgg gagcacgcag aatctgcacg aagttccatt cggcttgcgg gaactgcgcc
     6241 gaggcgatcc cgcggacggc gttgacgtcc tgaagcgagc tcaggaaaca cgtggcacgg
     6301 agaatgtcca cgggttgggc gccggcggcc ttcacggcgt cggccacgct ggcgatcgac
     6361 tttcgggcga gcggagctac gtcgagaagc atctcgtcgg aggccgccat ctgcccggag
     6421 atgaaggcga tgccgtgcgg attctccgtc ttcctcgcga cgccggtggc ttcgatcaca
     6481 acctgggccc cctccattgg gagagcgccg acctgaacca cgctgagcac cggaagcgga
     6541 aggcggcgct cggtgaacga ctcgctcacg atcgtctgaa cgcgccgcat atctccggtg
     6601 ccggcgacga aggcgcggag tttcacgatg gcgccgcggc gggcggtgcg ttgaagagcc
     6661 ttgagcgcat cccggacctg ctgggagagc agtcctttgg gcgtcagcgg tgagacgtcg
     6721 aagaagatcc ggcttgtatc cgcctgaatg gtggccggag gatctttggg cacctcgagg
     6781 gtctgggtga cctcttcctt ctcgcctttc ccttttttcc tttgagcgga caatacggcg
     6841 gggaggatcg cggacaacgc gaaaagtatg aggacgaaga cggccggttt ccgcatcgcg
     6901 tttcggacac gattgtatca gaactggccg tatggaaatg gcttgccgcg gccagtccaa
     6961 tatggtatgg tctggatgga ggtttgatgc ttcccgcgcc ggtgctggac cccgaatccg
     7021 aagtacctct ataccgacag ctccatgaac acgtgcgcga gctgatcgga gcgggccgtc
     7081 tcaaacccgg tgaccggttg ccatccacgc gggacttggc tggccatctt ggcctgaatc
     7141 ggaccaccgt ctccgctgct tacgaactgc tcgaaacgga ggggctgatc cgtgggcacg
     7201 tcggacgcgg gagtttcgtg accggcagcg ttccgtccga atcgtccggc ctcgactggg
     7261 cggaagtgct cgaatcgtgg gaccgcggct cgtttcccca gcctgcggcg gcacccgaag
     7321 aaatgatcag cttcgcgacg tcgcggcccg ccgaccattt gttcccgatc gacgagtttc
     7381 gcgcgacgtg cagggaagtg gttgccgcgg aaggcgcggc ggcgatcctg cagctcggct
     7441 ctccgagcgg ctacggacct ttgcgggact atctgatcga cgagggccgg gaggaagggg
     7501 tggcggggcc gggcgacgac gtcgtcgtca ccaacggatg ccagcaggcc atggacttga
     7561 tccagcgggt actcttgcgc cccggtgaca cggtgctgct tgaagatccg gtctacccgg
     7621 gagcgaaagg cgtattcgcg cgcgctggga cgcgcgtcgt cggagtgccg gtcggagccc
     7681 aaggcatgga cctcgaacat ctggagcgcg cgatagcgcg ggagaagccg cggctgctgg
     7741 tcgtgacgcc gaattttcag aatccaacgg gagcgactct tccgctggcg gcgcggcgga
     7801 cgattctgga gatggtccgg gaagcccgcg tggtcctcgt cgaaaacgat atctacggag
     7861 cactgcgata cgaaggcgag ccgctgccga cgctgaaaca gctcgatgac acgggcgaca
     7921 ccgtgctcct gcgcagttac tcgaagctgg cctttccggg tctgcgggta ggctgggtca
     7981 ccggaccgca cgcgctgtca gggaggctgg cggaggcaaa acagttcacc gacctccact
     8041 cggaccagct ttcccaggcc gtgctgctgc gatttgccga gtcgggccgg ctggcggcgc
     8101 accgcgcgcg gattgtcgcg gcgggggcgg agcggctgtc ggcggtgatc gaggcgtgcg
     8161 ggcgctacct gccggacggg acccggctaa cgcggccgca gggcggaatg aatctgtggg
     8221 tccggctgcc gcagccgctc gacgcgggag aactgctggc gcgggcgcat caggagaacg
     8281 tgagctattt gccggggaag tacttcgagg tcagccgtca cgatcccggc ggtttgcgtc
     8341 tgagtttcgc gggacttacg ccggagaaga tccgcgcggg cgtcgccatt ctgggcggca
     8401 ttttttcaaa tgaattggag cgggcgcggg cgtcgcgtca ttttgacccc gcgccggcga
     8461 tggtatagga ggcagtcgaa tgtttttgga cgatacgttt cgggtaaagg tcggcctggc
     8521 cgagatgctg aagggcggcg tgatcatgga cgtgaccaac gccgagcagg cgctgatcgc
     8581 cgagaaggcc ggcgcggcgg cggtgatggc gctggaacgc gttcccgccg acatccgcaa
     8641 ggaaggcgga gtcgcgcgga tggcgccgat ccgcaagatc cgcgagatca tggaggccgt
     8701 cagcatcccg gtgatggcga aggctcgcat cggacacttc atggaggggc gcgtgctgga
     8761 gtcgctcgaa gtcgattaca tcgacgagag cgaagtgttg acgccggccg acgaagagca
     8821 ccatatcgac aagcgccagt tcaaggtgcc gttcgtgtgc ggggcgcgca acctcggcga
     8881 ggcgttgcgc cggatcgcag aaggcgccgc catgatccgc acgaaaggtg aggcgggctc
     8941 cggcaacatc gtcgaggccg tacggcatat gcggacgatc cagaaggaga tgaagcagct
     9001 cacggtgctg ggcaaggaag agctggtggc ggagtcgaag aagctccagg cgccgctcga
     9061 actggtggag tacgtggccg agcacggcaa attgccggtc ccgaacttct ccgcgggcgg
     9121 catcgcgaca ccggccgacg cagcgctggt gctcgcgctc ggcgccgaag cggtttttgt
     9181 gggctcggga attttcaaga gcgacgatcc ggacgcgcga gcgcgggcaa tcgtgaaggc
     9241 gacgacctat tggaaagacc cggcgaagct gctcgaagcg agcgaggatc tgggcatggg
     9301 gatgccggga ttggacgtct cgaagctcca ggaatcggag ctgctgcaaa cgagaggttg
     9361 gtaagcgatg gccgcgaatg ggcgcgactg aacgcaactt cgcgttgatt cacggctttc
     9421 atcagggctg tttatgaaga aagtcggggt gcttgccctc caaggtgatt tcgaggcgca
     9481 tcagaaggcg ctcgaccgcg cgggtgccga ggccattgaa gtgcgaaccg cggctgaact
     9541 ggagggggtg gacggtctgg tgatccccgg cggcgagagc acgacgatgc tgaaactgct
     9601 gcgggaggag aagctcttcg agccgctgcg gcggttcggc gaacggcggc cgatcttcgg
     9661 tacctgcgcg ggaacgattc tgctggccac cgaggtatcg catcccacgc aggagtcgct
     9721 tgggctgatg gacatctccg tcgagcggaa cgggtatgga cggcagctcg acagccacgt
     9781 cgccgtcggg tgtccggaag atgagtttct tcggcggacg ggcgagggcg atctcgagat
     9841 ggtcttcatc cgcgcgccgg tcatccgccg ggcgggcggc gatgtgaagg tgctggtgga
     9901 acaccgtggc gcggcggtgc tggtggagca gggcaggcac ctggcggcga cgttccatcc
     9961 ggaactcggc gccgacgaac gggtgcaccg tctcttcctc gcgaagcttt gagaacaata
    10021 cggtgagagt tcttttcgtc tgccttggaa actcgtgccg cagccagatg gccgaggggt
    10081 tcgcgcgggc tcatggcaga ggagtttggg gagccgacag cgctgggctg atgccggcga
    10141 cgatcgtcgc gccactgacg cgccgcgtga tggaggagaa gaacatcagt cttgaagagc
    10201 acttcccgaa agggctcgac gagattcgtc tctccgacta cggcttggtg gtgaatatga
    10261 gcggctacga gatgcccggc atcgtcacgg cgccggtgcg tacgtgggac gtccgtgacc
    10321 cgatcgggca aagcgagaag gtataccgcg aggtgcgcga cgagatcgag cggctggtac
    10381 tgggcctgct gtcggaggtg cgtgcccgca ccggcgcggg gtaggcgtta aagtcgtgcg
    10441 gaaaccgaat agaatggtcc ggacggggta cgatggctgg cgacgcattt caggcttatc
    10501 ttctggcgca aattgaagga cgagaggaac ggtttccgct ggaaaccggg atggtctgcc
    10561 ggatcgggcg gggcgaggag aattccgtcg tgctgggcga caaccttgtg tcgcgccggc
    10621 acgcgctgtt gcagtgcacg caaggcggcg aatacttcct ggtggactta ggcagccgga
    10681 acggtacgtt gttgaacggg cgccgcgtca cggtgccggt gactctgaag acaggcgacc
    10741 ggatatcgat cggatcgtat gagatcgagt ttcaggggcc tccggcgata gcggaggata
    10801 aggccagctt cggcgagacg atgctggcgg tatcgcaccg gctgatcacg gtgctggtcg
    10861 cggacatccg cgacttcacc gggctctccc gccggattgg ggaggcgcga ttatcggaga
    10921 tgatgagcgt cttcttcggc gagtgcggga gggtgctggc ggagcacggc gcgtgggggc
    10981 agaagtacat cggcgacgcc gtgatggcgt tctgggtgca ccagcgtgag atccccgacc
    11041 gccgggaact ggcgggtgta ttcgcctcgc tagcggaaat cttcgacacg gcggccgggc
    11101 tgcagaagcg ggtcgggctc gacgcggcga tccggattgg cgcgggagtc aacacgggct
    11161 tggcttcggt ggccaatatc gggagtgtgg ccgcgtccga ctacaccgcg gtgagcgaag
    11221 ctgtgaacct ggcgttccgg ctggagacgg cgaccaagga ggtcggctgc gacgtggtgc
    11281 tgggccagga gacggtggcg cgccttgccc agtttgccga cgtctctcat ttgtttgaca
    11341 tgcgcaccgt gacgttgaag ggttacgaaa acccgatgcc ggcctacgcc gggcgcgccg
    11401 aatcggtcgc ggagcttcgc cggatccttg cgtagttgcg gcagagcgat gaaaatttga
    11461 cagcggcgca acgcttcggg acaatagaaa cgaattcacc atccgtgcgg atgtggcgga
    11521 actggcagac gcgcatggtt caggtccatg tgcccgcaag ggcgtggagg ttcaagtcct
    11581 ctcatccgca ccaaccaaat caataactta gagactttcg aggcccggcg ggtacttgtt
    11641 tttcagcagt cggcaccggg aactcagcca tcaatcgcga accgtcccta ttgcggcggg
    11701 cgttcaagct tgcagcgaaa tgcaccccgc cgaaagtgag ctgggttcgg ttcatcccga
    11761 tgctcgagga gaacaacatc aggagaacaa cgtcaggcga ggtttctcga gctcgaccct
    11821 tatctaaagc ttcaagcgga gctaccagtg aaggtgcagt gtcccccgtt tcggtgcatc
    11881 agccgccgcc atgccgcatc gacgtaccgc gcccgcgcag gatcgtctct cgcatcccga
    11941 gagacggggg tcctgtaccg cagcttgggc caggcgtaga tgatgggcgc tcttcggcct
    12001 gaatcgtcgt ctgacgactc cgctcccgga agtcttgcgg tcctcagcgc catcggaaaa
    12061 acactgcagc acctcttggt ccgaaagtgc tacgcgagcg agaatgagcc atgagcccgc
    12121 gattgggatc tggtggaatg gcttagtttt gaggtgcttc ggcagattcg tcgtttgatt
    12181 gaccgcaatc gccagaagcc tgaggaatac ggcgaggttt gtgttatagc aacacaaggc
    12241 atgcgttatc gtaatacaaa cctccggggg tgccccggca cgtcgtatcg gccgtaatct
    12301 ccaaacccgt ccgaacgatt cttcgactcg ttaccttgcg gtgtgatcgg cgggtgggcc
    12361 gccgaaagtg gaagcgatcc cgtctgccga cagttttctg gtgcattgtc cgaagcccaa
    12421 actcgaaagc cggccaagtg ctatgcacgc cgcccctgtt ctgtaaggga cacctacaag
    12481 agccgttgcc gtgcctcatg gagcgtcgca agtaggtgat ccagatcaat cagccacggt
    12541 tgaccgttcg gtttcggata
//
LOCUS       DATIFQ010000196         6298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1214970, whole genome shotgun sequence.
ACCESSION   DATIFQ010000196 DATIFQ010000000
VERSION     DATIFQ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214970"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VLE22_09070"
     CDS             <1..1077
                     /locus_tag="VLE22_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14598.1"
                     /translation="LSMQMGVAVDRIDAPFGAELELQNEIAIPPPSLDHRENSSFVAT
                     AELLAEGKRVRWGSKGEIHVESTAGTAEFEKAAWELRWPRVALYEPWTANIDTGWTQW
                     LLDSYRIPYTVLHNGDVAAKDLRARFDAIILASQSASSILNGERFGEASSGREIEDRA
                     SPEVKTAQRPEYTGGIGPRGLAALDDFARDGGTLIALDDAADLPVQFFPLSVRNVAQG
                     SGDTVRFECPGSLLRITVDRSNPIAFGMPEEAVAFSTGGHAYEIPLLAEFNKGEREIR
                     SVARYAKTNVLASGWVSGEKTVQGKHVLLDARHGRGRVVVFGFRPQFRGQPFGTFKLL
                     LNAIYLASARPLSGAEGPSGQSGR"
     gene            complement(1074..2999)
                     /locus_tag="VLE22_09075"
     CDS             complement(1074..2999)
                     /locus_tag="VLE22_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14599.1"
                     /translation="MRRIAFLLFPILLTISTLRAGNAVSAGRFVVEPPTLICLGFEWY
                     ISGDDNRNATVEVRYRKAGAIEWFDALPLLRIGGERILNPTVYLDYTTPQMFAGSILD
                     LEPDTEYECRLEMRDPDGVTGGTVQTAKVRTRGEPKAAADGRVLHVYPPNWKGKKEEP
                     AFTGLMKAYQGAGGGDWAIVGERKVKPGDIILVHAGLYRGDRLEYAHPLGLTFDGTYY
                     LTARGTSERPIVIRAAGDGEVIFDGDGCHRLFEVSAADYHIFEGLTVRNTDIAFFAGM
                     KDITGSKGLTVRNCRIENVGIAVTTQWAGSRDYYIADNVIIGRDDRYRLLGWFDPGIY
                     GANRLNSYNAVKVYGSGHVICHNFIAYFHDGIDVCTHGSPDPDEKPVAIDIYNNDIHL
                     MADDFIEADGGAHNIRIMRNRGVNAAQCGLSAQPVFGGPAYYIRNVLYHVPWGMAFKF
                     KVKPAGLLVYHNTIIAESRNGEPFSNAHFRNNLFLGADAPGREIFRFPNATSYSTYDY
                     NGYRPNRNAEKQYFWKSPAKGSLRDYDLDRKEFQAFRTLAELRDTTGHEVHGLEVDYD
                     AFESLKPADPAQPHAIYLGKELDFRLRAGGKAVDAGVRLPNVNDGYAGLAPDLGALEI
                     GRPAPVYGPRNGLRTDN"
     gene            complement(3054..3521)
                     /locus_tag="VLE22_09080"
     CDS             complement(3054..3521)
                     /locus_tag="VLE22_09080"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HSB14600.1"
                     /translation="MPSRKLKEFLDAHGVKYVCITHSVAYTSQEIAALAHIPGKDLAK
                     TVMVKLDGNLAMAVVPGSHKVDLRLLEAAAGARKAELSTESAFKSMFPDCETGAMPPF
                     GNLYGLPVFAAQSLSDDDEIAFNAGTHSELIRMPYPDFERLVQPKLGSFSIGP"
     gene            3728..4222
                     /locus_tag="VLE22_09085"
     CDS             3728..4222
                     /locus_tag="VLE22_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14601.1"
                     /translation="MRNMGAVLAVALLLLVGLVVFLFYGPMSEKPGMREVTADASEPG
                     PTAPQVKGPVAAKARRSRESPASEPETVEMAAANTPQTPLPAVSAPSSRAGRRFPTAA
                     DIPIGMERHRLQTGFGKPNMRTTVVEGGRLLETFVYLQAEPDVATFVLLRNGKVVSAN
                     TTIY"
     gene            4307..4732
                     /locus_tag="VLE22_09090"
     CDS             4307..4732
                     /locus_tag="VLE22_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HSB14602.1"
                     /translation="MSPDRSEILEAFKHSGLRCTSQRYAIFDYLVRHPVHPSAEQVYA
                     AVNRKDPRASLATVYKALHALAEAGLIRELTLDGNAIRFEAATTRHHHFICRACGRVE
                     DVDWFELPQFSCQVCIGARKLEGYDLVLHGLCETCSSAA"
     gene            complement(4744..5580)
                     /locus_tag="VLE22_09095"
     CDS             complement(4744..5580)
                     /locus_tag="VLE22_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSB14603.1"
                     /translation="MHNTRREFLAMTAAMLNAQTKPGMTKYVRFQKGSTTAYGILDGE
                     TIREIRGPLFGAPKETGTTHRLADVKLLYPCEPPKILAVGRNYKSHIGDLTPPTRPEI
                     FYKPVSALQNPGDPIEIPRGANNVHYEGELVVVIGRRVKNAGPDEAREAIFGYTCGVD
                     VSERDWQGGPDKDMQWWRAKGADTFAPLGPAIVRGIEGTGRLLQTRVNGKVVQQQSTS
                     DLLFDPARVISWISAYVTLMPGDVIYTGTPGTTSKLLPGDVLEVEIAGIGVLRNPVVA
                     AK"
     gene            complement(5591..>6298)
                     /locus_tag="VLE22_09100"
     CDS             complement(5591..>6298)
                     /locus_tag="VLE22_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14604.1"
                     /translation="GKHLHLEKPPADNLKDLNSLLATAEKKNLLVQMGYMWRYHPGIN
                     RALEAARKGWLGQVYLVRGMMNTLIPEKSRAEWALFKGGQMFEQGCHLIDPMVRLLGE
                     PEKVTPVLRKHGQFDDKLADNTVAVFEFSRALGIISSSVLQPGANRHRSFEVLGSNGT
                     AVVRPIEPGILNIDLAEAAGPYQAGIQRAELPPFRRYAGEFEELADKVASGQPLSVTP
                     KEDLLVQEALLLASEML"
BASE COUNT         1138 a         1921 c         1938 g         1301 t
ORIGIN      
        1 ctgagcatgc agatgggcgt ggccgtcgac cggattgacg cgcctttcgg cgcggaactc
       61 gagcttcaga acgagatcgc aattccgccg ccgtctttgg atcatcggga gaactcctct
      121 tttgtcgcta ctgccgagct gctcgccgag gggaaacgcg tccgctgggg atcgaaaggt
      181 gagattcatg tcgaatcgac ggccggcacc gccgagtttg agaaggctgc ctgggagttg
      241 cggtggccga gagtcgctct ttatgagcca tggacggcca acatcgacac tggctggaca
      301 cagtggctgc tggactctta ccggatccct tacacagtcc tccacaacgg agatgtcgcc
      361 gcgaaggacc tgcgtgcgcg attcgacgca atcatcctag caagtcagag cgcgtcgtcc
      421 attctgaacg gcgaacggtt cggggaggcc tcctcggggc gggagatcga ggaccgggcg
      481 tcgcccgagg tgaagaccgc gcagcgtccg gagtacactg gcggcatcgg tccgcggggg
      541 ctggctgcgc tggacgactt tgcccgtgat ggtgggaccc tgatcgcact ggacgacgct
      601 gccgatctgc cggttcagtt cttcccgctg tccgtgcgca acgtcgccca agggtccgga
      661 gacaccgtgc gattcgaatg ccccgggtcg ctgctgcgga tcaccgtcga ccgttcaaat
      721 ccgatcgcct tcggcatgcc tgaggaggcg gtcgcattct ccaccggggg gcacgcctat
      781 gaaatccccc tgctggcgga gttcaacaag ggcgagcgcg agattcgctc cgtggcccgt
      841 tacgcgaaaa ccaatgtgct ggcgagcggc tgggtctccg gggagaaaac cgtccaggga
      901 aagcacgtcc ttcttgatgc ccggcacggc cgcggccgcg tggtggtgtt cggtttccgc
      961 ccgcagttcc gcgggcagcc attcgggacg ttcaagctgc tgctcaacgc gatctatctg
     1021 gcttcggcac gaccgctgtc cggcgcggag gggccgtctg gtcagagcgg ccgttagttg
     1081 tctgttcgca gtccgttgcg cgggccatag accggcgccg gcctcccgat ctccagggct
     1141 ccgagatctg gcgccaagcc ggcatagccg tcgttgacgt ttggaagcct gacacctgca
     1201 tctaccgcct tgccgccggc cctgagtcga aagtcgagtt ccttgcctaa atagatggcg
     1261 tgcggctgtg ccgggtcagc gggtttcaga ctctcgaagg cgtcatagtc gacttcgagg
     1321 ccgtgtacct cgtggcctgt tgtgtcccgc agttcggcaa gcgtacggaa cgcctgaaat
     1381 tccttgcggt cgaggtcata atcccgcaac gatcctttcg ccggggactt ccagaagtac
     1441 tgcttctctg cgttccgatt cggacggtac ccgttgtagt cgtaggtcga gtacgaggtc
     1501 gcgttcggga atcggaagat ttcgcgccct ggcgcatcgg ccccgagaaa aagattgttg
     1561 cggaaatgcg cgttcgagaa gggctcgccg ttccggcttt ccgcgatgat cgtgttgtga
     1621 tagacaagca gcccggcagg tttcaccttg aacttgaacg ccatgcccca cggcacgtga
     1681 tagagcacgt tgcggatgta atatgccggt ccgccgaaca ccggttgggc gctcagcccg
     1741 cactgcgcgg catttacacc gcgattccgc atgatccgga tattgtgggc tccgccgtcc
     1801 gcctcgatga agtcgtctgc catcaggtga atgtcgttgt tgtagatgtc aatcgccacc
     1861 ggcttttcat ccgggtcggg cgaaccgtgg gtgcagacgt cgatcccgtc gtggaagtag
     1921 gcgatgaagt tgtgacagat gacatgacct gagccgtaaa ccttcaccgc gttgtagctg
     1981 ttcagccggt tggcgccgta gatccccgga tcgaaccagc ccagcagccg gtaacggtcg
     2041 tcacggccga tgatgacgtt gtcggcaatg tagtagtcgc gcgagccggc ccactgcgtg
     2101 gtgacagcaa ttccgacgtt ctctatccgg cagttgcgga cggtcaaacc tttcgatcca
     2161 gtgatatcct tcatcccggc gaaaaacgcg atgtcggtat tgcggacggt cagaccctcg
     2221 aagatgtgat agtcggcggc gctgacctca aagagccggt ggcatccatc gccgtcgaaa
     2281 atcacttcgc cgtcgccggc ggcacggatg acaattggcc tctcggacgt gcccctggcg
     2341 gtgaggtaat acgttccgtc gaatgtcaga ccgagagggt gcgcgtactc caaacggtct
     2401 ccacgataga gcccggcgtg aacgaggatg atgtcgcccg gtttcacttt gcgctcaccg
     2461 acgatggccc agtcgccgcc gcccgcgccc tggtatgcct tcatcagacc ggtgaacgcg
     2521 ggctcctctt tctttccttt ccagttcgga ggatatacgt ggagaacccg cccgtccgca
     2581 gcggcctttg gctcccctct cgttcggacc ttggccgtct gtaccgtgcc acccgtgact
     2641 ccgtcgggat cgcgcatctc caggcggcat tcgtactcgg tatccggttc caggtcgagg
     2701 atactgccgg cgaacatttg cggcgttgtg tagtcgaggt agacggtggg gttgaggatt
     2761 cgttcgccgc cgatgcgaag caaaggcagc gcgtcgaacc actcgatcgc accagcttta
     2821 cggtaacgga cttccacggt ggcgttgcgg ttgtcgtctc cggagatgta ccactcgaag
     2881 cccaggcaga tcagcgtcgg cggctcgacg acgaagcgcc cggcgctgac tgcgttcccg
     2941 gcgcgcagtg tcgagatagt caggaggatg gggaacaaga ggaaggcaat acgtcgcatg
     3001 gcggggcaat tataccccga tggcggtcac cgccaacgcc cgatggaccg ttgctacggc
     3061 ccgatcgaga acgatccgag cttcggctga accagacgct cgaagtctgg gtacggcatc
     3121 cggatcagtt cgctgtgggt gcccgcattg aaagcgattt catcgtcgtc cgagaggctc
     3181 tgagcggcga acaccggcaa tccgtaaagg ttcccgaagg gaggcatggc gccggtttcg
     3241 cagtccggaa acatggactt gaacgcggac tctgtggaga gttctgcttt tctggctccg
     3301 gccgccgcct cgaggagtcg gagatccact ttgtgagaac ctgggactac cgccatggcg
     3361 agattcccat cgagcttcac catcacggtc ttggcaagat ctttgcccgg aatgtgagcg
     3421 agggcagcta tctcctggct ggtgtaagcg acggagtgcg tgatgcagac gtacttgacg
     3481 ccatgggcat ctaggaattc cttcagtttt ctggacggca cgagcacctc ctttgagacg
     3541 tttctatatt acgctggaaa gggtgcaatg acgtccgctt tttcgagcgg caccgaggag
     3601 ggaaggggaa cagttacggg gtatgtgttg gacttggtac gggacgtaat agtactggcc
     3661 taccctttca agctggccgg tgaggcctta caataatcgt acgcacgctg cgtcggagga
     3721 ggcgttcatg cgaaatatgg gagcagtgct tgccgtggcg ctcttgctgc tggtcgggct
     3781 ggttgtgttt cttttctacg gcccgatgag tgagaagcct ggcatgcgcg aggtaacggc
     3841 ggacgcatcg gaacccggac ccaccgcacc ccaggtgaag ggaccggtgg cggccaaggc
     3901 aaggcgttca cgcgaatcac ccgcatccga accggagacc gtggagatgg ccgccgcgaa
     3961 cactccgcag actccgttgc cggctgtctc ggcgccttcc tcgcgcgccg gcaggaggtt
     4021 tcccaccgcg gccgacatcc ccattggaat ggagcgccac cggttgcaga ccggcttcgg
     4081 aaaacccaac atgcggacaa cggttgtcga aggcggacgg ttgctggaaa ctttcgtcta
     4141 cctccaggcg gagcccgacg tcgccacatt cgtgcttctc aggaacggaa aagtagtttc
     4201 ggccaacacc acgatctatt aaggcaccgc ggcacttcgc gaccttccac gcctgaggcg
     4261 cacgtattga caagttcttt cctgagaatt actctacgga gtggtgatgt ccccggaccg
     4321 atcagagatt ctggaagcgt ttaagcacag tggcttgcgt tgcacgtcgc agcgatacgc
     4381 gatttttgac tacctggtcc ggcaccccgt acatcccagc gccgaacaag tctacgccgc
     4441 cgtgaatcga aaagacccgc gcgcgtctct agctacggtc tacaaagcgc tacacgctct
     4501 ggcggaggcg ggcctgatcc gggagttgac tctcgacggg aacgcgatcc ggtttgaagc
     4561 agccacaacc cgccatcatc atttcatctg ccgggcttgc ggccgcgtcg aggatgtcga
     4621 ttggttcgaa ctcccccagt tctcgtgtca ggtgtgcatc ggagcgcgaa aacttgaggg
     4681 atatgatctc gtcctccacg gtctctgcga gacctgttcc tcggccgcgt gagcggtgtc
     4741 cggtcacttt gccgcgacga cgggattgcg taggactccg atacccgcga tctcgacttc
     4801 cagaacgtcc ccaggcagga gcttcgacgt ggtgcctgga gtgccggtgt agatcacgtc
     4861 tcccggcatc agcgtgacgt aggcgctgat ccagcttatg acccgcgccg gatcgaagag
     4921 caagtcggag gtggactgct gctggaccac cttcccgttg acgcgcgtct gcaatagccg
     4981 gccggttccc tcgattcccc ggacgatggc cggccccagc ggtgcaaatg tgtcggctcc
     5041 cttcgcgcgc caccactgca tatctttgtc cggcccgccc tgccagtccc gctcgctgac
     5101 gtcgacgcca caggtgtatc cgaaaatcgc ctcgcgagcc tcatccggac cggcgttttt
     5161 gacccggcgg ccgatgacga cgaccagctc accttcgtag tgaacgttat tggctccacg
     5221 cgggatttcg atcggatcgc ccggattctg gagtgcgctc accggcttgt agaaaatctc
     5281 gggccgggtt ggcggtgtga ggtcaccgat gtgactcttg tagtttcgcc cgaccgccag
     5341 gatcttcggc ggctcacacg ggtacaacag cttcacatca gccagccggt gggttgtgcc
     5401 ggtttccttg ggcgctccga acagcgggcc acgaatctcc cggattgtct cgccgtcgag
     5461 gattccgtac gccgtcgtgg aacccttctg gaaccgcacg tatttcgtca ttcccggttt
     5521 tgtctgggca ttcagcatgg ctgccgtcat ggcaagaaac tccctgcgcg tattgtgcat
     5581 tgccgattcc tcacagcatc tcactggcca acaacaacgc ctcctgtacg aggaggtcct
     5641 ccttcggcgt gaccgacaac ggctggcccg acgccacctt gtccgccagt tcctcgaact
     5701 ctccggcata acgccggaat ggaggtagct ccgccctctg gatcccggcc tggtaggggc
     5761 cggctgcctc cgcgaggtcg atattcaata tccccggctc gatcggccgg acgaccgccg
     5821 ttccgttcga tccgagcacc tcgaacgacc ggtgcctgtt cgctcccggc tgaagcaccg
     5881 aactcgagat gatgcccaag gctcgcgaga attcgaagac cgccactgtg ttatcggcca
     5941 gtttatcgtc gaattggccg tgctttctga gcacaggcgt tactttctca ggctccccca
     6001 gcagccggac catcgggtcg atcaggtggc acccctgctc gaacatctgc ccgcctttga
     6061 aaagcgccca ttcggcacgg ctcttttcgg ggatgagggt attcatcatc ccccgaacga
     6121 ggtagacctg gccgagccac ccttttcgag ccgcttccag cgcgcggttg atgccgggat
     6181 ggtaacgcca catgtaaccc atttgcacca aaaggttctt cttctctgcc gtcgcgagca
     6241 gcgagttgag atccttcaaa ttgtccgccg gcggcttctc gaggtgcaga tgcttgcc
//
LOCUS       DATIFQ010000197         2571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_831300, whole genome shotgun sequence.
ACCESSION   DATIFQ010000197 DATIFQ010000000
VERSION     DATIFQ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831300"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VLE22_09105"
     CDS             <1..668
                     /locus_tag="VLE22_09105"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB14605.1"
                     /translation="FTVCSAQAPDDNSGFEPIFDGKTLTSWDGEPGFWRVENGAIVGE
                     TTPEKALKQNTFLVWRGGTVKDFELKLEFRLTASANSGIQYRSAIVPEVSRWAMKGYQ
                     ADMDGANKYTGMLYEERARAFLAPRGQFSRMAEDRTLKLIATLGEADALKTFIKTGSE
                     WNHLHVIARGNTLMHFLNGHLMAAFIDEDPHGRAMEGLLGLQLHAGPPMKNEFRNIYY
                     KKL"
     gene            complement(719..>2571)
                     /locus_tag="VLE22_09110"
     CDS             complement(719..>2571)
                     /locus_tag="VLE22_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14606.1"
                     /translation="PVIDLDSGAIYVVANNVEHGLYFFRLHALDITTGEEMFGGPVDI
                     SASVPGQGQGSQDGMVSFVPFDHLQRPGLLLLDNVVYASFGSHQDRPPYHGWLIGYDA
                     RNLQRQAAVFNVTPDGEEGSIWQSGRAPAADDLGNIYITTADGDHNGTTNWGESVLRL
                     GAAGGLTVADWFTPDNWMDLNLHDDDLGSCGPVLIPGTNLLVSGSKRGAVFVLDRNNL
                     GHLSAGNSGTIQSFQAVNFGLFNSAALWNNADGPILYIRGQGQFLTAFRMSGGLFNPT
                     PMSESTTAGPISHDGVAVSADGSTAGTGIVWNTRPIDYSHPTPGILYAYDALDLRHEL
                     WNSELNPARDRLGLFAKFATPTIAGGKVFVPTFSGELAVYGLLTSQRQIKGIVNAASL
                     TSGPVSPGEIVNIYGAGFGPASPAGARLDSSGRLTTSLAGVRLFFDGAPAPMLFAWRG
                     QLRVVVPYSVSGRGSTRVQIDDGGQVSGVFPAPVACCAPGLFTTGVSGVGQGAILNED
                     FTVNSEENPATRGSIVMLYGTGEGITDPPAVDGKSAGFPLHVPVAPVSVSLGGEDAAV
                     TYAGAAPGLVSGVIQVNVRVPEGIQPSPTVPVLLKVGDAASQPDVTLAVR"
BASE COUNT          498 a          781 c          832 g          460 t
ORIGIN      
        1 tcttcaccgt ctgctctgct caggcgcctg acgataactc cgggttcgag ccgatcttcg
       61 atggcaagac cctgacgagc tgggatgggg aaccggggtt ctggcgagtg gagaatgggg
      121 ccattgtcgg cgaaacgacg cccgaaaagg cgctaaagca gaacacgttt ctcgtctggc
      181 gaggcggcac ggtcaaagac ttcgagctca agctcgagtt tcggctcacc gcctcggcca
      241 acagcggcat ccagtatcgc agtgcgatcg tgccggaagt ctcgcggtgg gcgatgaagg
      301 gctaccaggc cgatatggat ggagccaaca agtacaccgg catgttgtat gaagagcgcg
      361 cccgcgcatt tctggcgccg cgcggccaat tctcgcgaat ggccgaggac cggacgctga
      421 aactcattgc gactctcggc gaggcggacg ctctgaagac gtttatcaaa actggctcgg
      481 aatggaatca tctccatgtc attgccaggg gaaacaccct gatgcatttc ctcaatgggc
      541 atttgatggc ggcgttcatt gatgaggatc cccacggccg agccatggaa ggcctgctcg
      601 gcttgcagtt gcatgccggc ccgccgatga agaacgagtt ccgcaacatc tactacaaaa
      661 aactctaagt cgtgtagcgc tcgaggaccg ttcagccaat ttgaaccgcg cgcccggctt
      721 accgcacggc aagagtgacg tcgggctggc tggctgcgtc gcctaccttc aacaacaccg
      781 gcacggttgg gctgggctgg atcccttccg gcacgcggac attcacctgg atcacgcctg
      841 ataccagccc gggagccgcc cctgcgtatg taacggccgc atcctccccg ccgagtgaga
      901 ccgacaccgg cgccacggga acgtggagcg gaaagcccgc cgacttgccg tcgacggccg
      961 gcgggtcggt gatgccctcc cccgtgccgt agagcatcac aatggagccc cgggtcgccg
     1021 ggttctcctc cgaattgacg gtgaagtcct cgttcaggat cgcgccctgg ccgacgccgc
     1081 tgaccccggt ggtaaagaga cccggcgcac agcaggctac gggcgccggg aacacgccgg
     1141 agacttgtcc gccgtcgtca atttgaaccc gggtgctgcc ccgcccggag acggagtacg
     1201 gtacgaccac ccggagttgt cctctccagg cgaaaagcat cggagcagga gcgccgtcga
     1261 agaacaagcg aacaccggcg agactggtgg tgagccgccc cgaggaatcc agtcgcgctc
     1321 cggccggact cgccgggccg aatccagcgc catagatgtt cacgatttca cccggggaca
     1381 ccgggccgct cgtcaggctg gccgcattga cgatcccctt gatttgccgc tgcgaggtca
     1441 acagaccata gacggcgagt tcgcctgaga acgtgggcac gaagaccttg ccgccggcga
     1501 tggtgggcgt ggcgaatttt gcgaacaagc cgagcctgtc ccgcgccgga ttcaattcgc
     1561 tgttccaaag ctcgtgtctc aggtcaaggg cgtcgtaagc gtagaggatg ccgggcgtgg
     1621 gatgggagta gtcaatggga cgtgtgttcc agacaattcc cgtgccggcg gtgctcccgt
     1681 cggctgagac ggcgactccg tcgtgagaaa tcgggccggc ggtcgtgctc tccgacatcg
     1741 gcgtcggatt gaagaggccg cccgacattc ggaacgctgt gagaaattgg ccctggccac
     1801 ggatgtacaa gatcgggccg tccgcgttgt tccagagggc ggcgctgttg aagaggccga
     1861 aattgacggc ctggaagctt tgtatggtcc cgctgttccc agcgctcagg tgtcccaggt
     1921 tgttccggtc taagacgaag acggctcctc gcttactccc ggagacgaga agattggtcc
     1981 ctgggatcag gaccggtccg caagagccga ggtcatcgtc gtggaggttc aagtccatcc
     2041 agttgtccgg cgtgaaccag tcggcgacag taagtccacc ggcggcgccg agccggagaa
     2101 cgctttcacc ccagttagtg gtgccgttgt ggtccccgtc cgccgtggtg atgtagatgt
     2161 tcccgagatc gtccgccgct ggcgcacggc ccgattgcca gatggatccc tcttcgccgt
     2221 ctggggttac gttgaaaacg gccgcctgcc tctggagatt ccgcgcgtcg taaccgatca
     2281 gccatccgtg gtagggcggc cggtcctgat gcgacccgaa ggaggcatag acaacgttgt
     2341 cgagcagcag gagacccggc cgctgaaggt gatcgaacgg gacgaacgac accattccgt
     2401 cctggctccc ctgaccctgg ccgggtacag aagcgctgat gtcgacgggg ccgccgaaca
     2461 tctcctctcc ggttgtgatg tccagcgcat gcaggcggaa gaaatacaag ccgtgctcga
     2521 cgttattggc gacgacgtag atggccccgg aatcgaggtc gattacggga g
//
LOCUS       DATIFQ010000198        22527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_257415, whole genome shotgun sequence.
ACCESSION   DATIFQ010000198 DATIFQ010000000
VERSION     DATIFQ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 22527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22527
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257415"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VLE22_09115"
     CDS             complement(<1..847)
                     /locus_tag="VLE22_09115"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HSB14607.1"
                     /translation="MKTLPFLLEIGTEEIPDWMIRPALEDLGQLFRDVLAANKLSGDV
                     TWVDATPRRLVLKADGLLARQADSKERVTGPPKAAGEGAANGFARKLGVEVSALKTVR
                     TAKGEYLSYERKVRGRQTAEILSEALPGLILKIHFPRAMYWTGKGGPRFIRPIRWIVA
                     LLGGKVVPFELAEVKSGNATAGHRQLGKKKVRVAVANYEEQLRQNGVIVSAAGRRRKI
                     SGEIEALLAGRGLTVKADEALLDTLVYLTEQPAPILGSFDARYLELPEEVLVTVMRHH
                     QRYFSV"
     gene            complement(844..1731)
                     /locus_tag="VLE22_09120"
     CDS             complement(844..1731)
                     /locus_tag="VLE22_09120"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HSB14608.1"
                     /translation="MDSFQETLFKLKNYWARRGCVIQEPYDVEVGAGTMCPETFLRVL
                     GPKPYRVAYVQPSRRPADGRYGENPNRLYKHSQLQVILKPSPDDVMDQYLDSLEAIGI
                     DLGKHDIKFEEDNWESPTLGAWGIGWQVMLDGLEVTQFTYFQQCGGVDLDLVPAELTY
                     GLERLTAFLQGKESVYEIEWAPQASYGDVRLADELQFSVYNFEMADVGTLWQLFDLHE
                     KECRRLLDAYPDYADKKRFPVLPAYDHVLKCSHLFNLLDSRGAISVTERVGVIGRVRK
                     LAVGVAQAWLDQNAAEAAA"
     gene            complement(1915..2580)
                     /gene="recO"
                     /locus_tag="VLE22_09125"
     CDS             complement(1915..2580)
                     /gene="recO"
                     /locus_tag="VLE22_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HSB14609.1"
                     /translation="MCYIVLAEMPARESESLILRTYPFQEGDLIVSFLARDLGKLRGV
                     AKRARRPKSPFGAGLERLSHVRLSYFQKESRELVTLDSCELIRSSFEFQSDYAAAVTL
                     DYVAEVSEQLLAPAEHSERFFRLLLAVLDHFWALRSEGVWSAVLYFTLWSVRLSGFLP
                     ELKASHSSLELATEILTTPIGALAPRQWTRETAADLRRFLVRRIEEHIERRLITAPLL
                     ESL"
     gene            2609..2902
                     /locus_tag="VLE22_09130"
     CDS             2609..2902
                     /locus_tag="VLE22_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14610.1"
                     /translation="MSTPKFPLPDHPAPVSELVDTLSDGTKVKRRVLRQRACNEKDEK
                     GKVCAGHLKRWYFFGDEVKQKFGSEAEIYRCENCKTLYLPNLEEEPRSGTLRW"
     gene            3447..4811
                     /locus_tag="VLE22_09135"
     CDS             3447..4811
                     /locus_tag="VLE22_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14611.1"
                     /translation="MPLRSAPCLLLILMLVATAWPQVVEEEYRVYTEHPRLFLNTKRL
                     RLLKRERERSSLRWQQFDALMSGGARMPEPGFAHALYSQVTGDPAHCRIAIDWALGPA
                     ADLRQAALVFDWCESRLTGDESARLGARIRTWIGKAATMHEVAPTRDRALAAIALADH
                     DFPASEQYLRGIIRKWWRGEMAPALQSGKKNLNSEDVYPLLEILHAVQDNLKIDLREN
                     APGFFRDLPINRLLSYYPASYPAPENEYRIPAWTGDGEPDLDVAALSRAADLAMTAYD
                     ANMREHQFLQGWLIHDRFLLRSAFGTPYEFLWANPYQPGLSYYHMPLAQHDTRRGLLF
                     LRSSWDDDAVWLGAFDGEIQLFENGQRKVVKAGPGAKPVRIGDVVAVFASGASRFNIN
                     DPEAKAVFIVGLKPGKAYEIEVDDEEMREISADAGGVLAIDIFPRPRLGLRLRPAPDA
                     RPSS"
     gene            complement(4808..5569)
                     /locus_tag="VLE22_09140"
     CDS             complement(4808..5569)
                     /locus_tag="VLE22_09140"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB14612.1"
                     /translation="MRFDNRVAIVTGAAQGIGFGIAERFLEAGASVAAVDVLADRIQD
                     AAEQLGSKGPALAIAADVSVEEQVEAAVEETVKRFGRLDVLVNNAGIEVLGSASEISA
                     KGWDRQLDVNLRGAFLCSKYALEKMRGRGGAIVNIASVHAFASYPGVVAYDASKAGML
                     GMTRAMALDHAKDAIRVNAICPGYIDTPMLDAWLKTMPDRHEAMRGILKCHPLGRIGT
                     PRDIAEAVLFLASEAASFITGATLVVDGGMTIQGH"
     gene            complement(5576..6295)
                     /locus_tag="VLE22_09145"
     CDS             complement(5576..6295)
                     /locus_tag="VLE22_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB14613.1"
                     /translation="MKSVALLFLLGALVTAQTKRKTDEFPTAEGPLRITPVRHASLML
                     EGGGQVVHVDPWSQGNYEGLPQADLILITDNHGDHLDAAMVDKLKKARTPVVGPEVVA
                     AKIPGAVPVRAGQTKTFGKWTVEAMPAYNLKRGPAEGKLFHEKGRGVGYLLTFGGKRI
                     YVSGDTEGIPEMKALRDVDIAFVCMNLPYTMPPEEAAEAVRAFKPKVVYPYHYRGSDL
                     LLFEKGLAGTGIEVRNRDWYY"
     gene            complement(6378..7376)
                     /locus_tag="VLE22_09150"
     CDS             complement(6378..7376)
                     /locus_tag="VLE22_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HSB14614.1"
                     /translation="MGLAKDATLGKPRKTVDAGSVGLIGLGLMGQGIASCLLAYGFRV
                     IAYNRTASRAKASIDHVSAALREMVKRGLRTPAQVRGWRDRYELAESVGQLAPCGFVV
                     ETVKEDVELKRRIYDELEAAVKASTVISSNTSSIPITLLQEGRRRPERFIGMHWGEPV
                     QVMRYLEIIPGQATAPRAVRLARKIGIACGKDPTVLNTDIRGFLSNRMMYAMLREAFH
                     LVEHGIASLEDVDRSFRNDIGWWATIAGPFRWMDLTGIPAYGTVMEGLMPELCNASEV
                     PQLMKDVVGRGSQGISNCKGFYKYDKAGAAKWEEAWVDFTYDLRKLVEKYERRVKL"
     gene            complement(7367..9514)
                     /locus_tag="VLE22_09155"
     CDS             complement(7367..9514)
                     /locus_tag="VLE22_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HSB14615.1"
                     /translation="MTCRILVLSVLISSLFLANPAAEEIPKQPGVHGRYGIVIIPEPQ
                     IVKVTEDSFQPDANTAVYQTPGGQDSSAVASLIEGVEQTRGLWLRTAGSRPDSNGIIL
                     SIVPESRLEGVPPGSARKEAYILAVTQKGVSIEAAFPQGLFYGVQTLLQLLQQGKGNI
                     RGLRILDWPDMEFRAIHVDLWYHLDRPWYYEHLFRQLARYKINTAVFEFEDKFQYTRH
                     PVLSAPGALTHEQVRQLVQTATKYHIEIVPLVQTLGHVSFIAKHPEFQHLREVPKSNW
                     QLCPLKEGTFGLIREMLDELLDVVEPAKHVHIGGDEARELGMGPECRAKWGDKAPVES
                     YKLWLTFVCDLLKKRGKTAIVWDDMFLRHFNAADMARLPDNLIYMRWDYDAGKFVDRD
                     KLILNLKYPVWVATAAQTMTPIFPDQRLRVYNNANFIPDAVALGVKGVLNTAWEDEGI
                     HPETYWIGFVCSAEYSWSGKKPTTQEFEAKFFPLFYGPRQTELAEAYDILSEKGFIRR
                     ESGWTREFGALALPPLPDAAFRVDTSWGQKHASLLVQAQQRLPRYERATEIIKANLAR
                     DPENRYNLEVLLLCARTMLHFTKLVLSIGEIDENLQAAMHDHQKGDDQAAINRYHRIG
                     RIIDDLRHEKSALFDETVQVWEKSTYPKDFRHVPGGRDKFVHQIDRDFYYGNKTMDLS
                     YIFEVEEKLPLFAYQQSLYQIIVDNVRGEHPWD"
     gene            9696..11210
                     /locus_tag="VLE22_09160"
     CDS             9696..11210
                     /locus_tag="VLE22_09160"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HSB14616.1"
                     /translation="MENIVLGNGTLIDGTGSRPTTGDVLIRGDRIAEVGRFEKPAEAR
                     LIDCSGLTVAPGFIDAHSHSDLQVLENREEKVRQGVTTEVVGNCGFSPYPAPDDRSLL
                     QGFANGIFCGDDRWGWRSARDYLKDVERSPIVNVASLVGHGSLRISVAGHRLGPLPER
                     DVEAMEEKLSDSLSEGASGLSTGLMYAPGSSAPFEELERLCRVVAKHDKIYTTHMRSY
                     FSGLVDALDEQLDLARRTGCRLQISHLQAVGASNWPLQARAIDKIEQARRDGIDIAFD
                     CYPYVAGSTVLTQALPQWVLEGGVVGMLARLTDPSQRAHIAAETKSALEWRWVDIYIS
                     AVGSAANQEAVGQNLTALAETRGCEPVDVMLDLLIEERGAVNMLCFNQSEENLKQTLT
                     HPLSMIISDGFYVKGRPHPRLHGTFPLWLGDICRERRWLELPEAVHKITDQPARRFGI
                     KNRGRLLPGYFADVTVFDPKNVGSPATYDSPEQAPIGIHHVLRNGKSQFGGGAV"
     gene            complement(11227..12315)
                     /locus_tag="VLE22_09165"
     CDS             complement(11227..12315)
                     /locus_tag="VLE22_09165"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSB14617.1"
                     /translation="MRITDLDTPAVLVDVDVMERNLRRLGDYCAQYSLRLRPHTKTHK
                     IPELARRQIDHGAAGITVAKLGEAEVMADAGLPGILIVYPLFGGAKWRRLAALARRAD
                     ITVACDSVEIANGISEQARQDCVTVGLLVEFDAGFGRCGLPIDESSLPAVRKIRELPR
                     LEWRGVLVYPGHIMGSASEREVLLKRENETLSRLMELLKSEGIDCPIVSGGNTPAAFL
                     SHRFVGINEIRPGTYIFNDKNTVCAEAASWDDCAVTVLATVVSTSVSGKAIIDAGGKT
                     FSGDSLLSGDRQGFGHVIGHPEILVEDLSEEHGHLRLGGARLKIGDRVRVVPNHVCTC
                     VNMHDRVYGVQHEDVVAEWEVAGRGKVR"
     gene            12399..13241
                     /locus_tag="VLE22_09170"
     CDS             12399..13241
                     /locus_tag="VLE22_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HSB14618.1"
                     /translation="MAASKTPTVSSVERTLHILELLATSRAGLTLPEISRRLGLPKSS
                     AHCLLLTLERSGYLTSNTTTRRFMFGLKLFSLANMAHSGVRLREQARPFLRSLVNETG
                     LTVHMGIRERDEVVLVEKIGPAAAPKLATWLGKRMDLHCTGVGKAVLAHLPAEELESF
                     IRARGLPRHNENTIASIRRLREKLAEVRSTGYALDDEEDEIGTRCIGAPIFDPAGTAI
                     AAVSLAGTTDQITSDNLGHFAGLVKQTAGAISHTLRPEESPASEEPSEPPAQERENSI
                     AQGI"
     gene            complement(13269..14264)
                     /locus_tag="VLE22_09175"
     CDS             complement(13269..14264)
                     /locus_tag="VLE22_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HSB14619.1"
                     /translation="MGKRNKSRGNIDPSEITAGVVGLGLMGRSIVTCLLASGHPVVGI
                     TGTQSKLKTTRRQVLALLKELQEEGILKEDPSAVAERLTISTDFSALRKAGVVIESII
                     ESVKAKKEIIRNVEQVVSRRALLGTNTSAIPVTQLQEGALHPERILGFHWAEPAHVTR
                     FMEIICGKQTRPASAAHAMALAHRWGKEPSLLRRDVRGFITNRCFYAMIREAFHLVES
                     GYATIEDVDRSLRNDLGYWITFAGPFRFMDLTGIPAYRTVMRDLWPELDCSTKVPRLM
                     KKVVDSGALGVANAKGFYRYTPAQAKRWERLFLKFSYDIRALALKYPEDIGDRAR"
     gene            14459..16729
                     /locus_tag="VLE22_09180"
     CDS             14459..16729
                     /locus_tag="VLE22_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HSB14620.1"
                     /translation="MFLPAALLLLPSVGLAAAPLIYPEPQQMSIAGGRFELDEKVSVV
                     IPQAASDRDLSLAGFLIAELSDRYGLALQTERVTAVPASGRVILMGSVENPLIRKYCA
                     RQNLTVSRDSPGPEGYVLHVGERVVLVAGSDDQGAFYGVQSLRQLVERGQGRSIQAVQ
                     VRDWPHKSFRGIKLYLPGRENFAFLKRFLRDFMALYKYNKLIVELNAAMRLDRHPELN
                     TGWAEMAKDLIYSRRDRSTGPNGEYQDSTHHDTGDGGILEKAEVADLVRWATEYNIEV
                     IPELPSLTHSYYLLTSHRELAEMPDAEWPDTYCPSNPKSYELLFDVFDEYIDVMKPKL
                     IHVGHDEWRMPWGVCPKCKGKDHRELYAQDVIKIHSYLARKGVRTAMWGDHLLEAVAG
                     PMLVDRKTRAGYKYRIPGGLTPDQVQKLIPKDILVFNWFWNDSERGSGETNEETLGKW
                     GFEQVYGNFTPEIRNYGRRSGRSGILGGAPSAWIATTEFNFGKDLIYDFAGCASLLWS
                     KHWPEAEQLSRIVQNMMPQIRRNLSGRATPSEDGEVVRMLPVLSLQASSAKAAEAVDT
                     AQVKSGRVASGRATFELPGKSMVAQASDGIPVGEDASSLIFLHALAKPASNEPGHRYI
                     YNFPDTADLLGWYEVVYDDGFVEIVPVRYGENILEWSWGRRHQPSTYCYRADPVDIGR
                     AAGNPVTFFAFEWINPRFGKTIREVRLKGTTGFRNTRGRVIPDNSIVLAAISSTKKRG
                     YPEPVKARTYVRQESP"
     gene            16769..17899
                     /locus_tag="VLE22_09185"
     CDS             16769..17899
                     /locus_tag="VLE22_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14621.1"
                     /translation="MSEPDRRRFLLGVAALGAGSLHALETSEIRTAFIGVGNRGKELL
                     RQVLNQASAPVAAICDTNPTARDEAQGLASRYSPRSLTEWRRVVDMKDVDAVMIATPC
                     NLHAEMAAACLDAGKYVYCEKPLGITAEEVDRVVKAARRSKAFLQIGQQMRYYPWLQG
                     AIPHIHNGLIGKPLIIKAQRHSTATPEQEKGHLPEWYFDPRISGDRIVENGVHNLDVC
                     NWIAGSLPVSVYGFGKTYLPKPKPPGEEYMDAYSVMYMYENEVNLTYSEAKLHPRALK
                     ELANGQWYVIFGEKGAVTIKRGSALFYEMSSESEPRELLTAEQQNAKEDAMGDFFASI
                     REKKQPLAGLRVAATAALTTIMGRKAIYERRMVTWKELGVSI"
     gene            17923..19770
                     /locus_tag="VLE22_09190"
     CDS             17923..19770
                     /locus_tag="VLE22_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14622.1"
                     /translation="MNRLFSSMSRLAAGFVLVVSCLSLTAQAASERPPLPYRFLLVIS
                     NQWKDPASILVEGGGEFQAVTSLLKTWGLPFDILRLDQQPLDRYHLIDREGHPLYGTI
                     IFDPDPAGLEGKDLGFLPALVKDFGVNMVVLGDAISAPEAAALAGLEYVSEYRLGDGM
                     TFAGEHFITRGLAGREKELLQGAGWEISGSKVVPKDATVLAKRAHLPFLTARETPGGG
                     RVAWLGAHRGSVQIAKQVTRDLFKRSLVWTQGYALYAEYPKSMILFMDDFGTSDRSYL
                     PYWHYKTLDEQDIRKGLIEPLKRHHAVINQDVLTGYIDRKGRRIANPWKQRVIDEIDG
                     KTVHDYVSAKRGLDAGLREGVFEVQSHGYTHMLPDLESPPGPFWTAPMDGIGTLGWDV
                     EFGDELRKREVPAITQRFFLSRGLAHIRQDFGVTPLFVINGGSAWSHSYTNNSPRIAA
                     EMGFGLSIFGKPGYLGRDLVIASMEPIVLNGGWAHDRKLTGDDISWTIDAPYFLVFHD
                     RDISIDPTAAERLLTGLGKGVRYLSANEYAGYLHAKIERDNSSGQSLALAVDYDEHYC
                     RYFATHESTWVLHLSDEMRSGLRATAEKQVITIPKGLGRHVVTAAGTSQ"
     gene            19782..20909
                     /locus_tag="VLE22_09195"
     CDS             19782..20909
                     /locus_tag="VLE22_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14623.1"
                     /translation="METAATSQEPGQAAASAPAASKRLVSLDAFRGWTMFWIVGGGAL
                     VTGLEALGHNPVIDTLVYQLEHTPWQGLRYYDLIWPSFMLMTGMSLPFSYAKRSLTQT
                     HREILLRVLRRVIVLLLLGSIRESVSLNHPYWVELSSALQPIAIAYFCAFLLVRKSWR
                     FQAAVGASILAGYAVLLAFVPAPGIPAGSYENGRNLVAAVDVAVLGRTHKEGWGTVIS
                     TIPTIATTILGLLIGELLRSTRPQKAKLRIMALVGIAGVVLGFALDPVIPIVMKLWTT
                     SYGLASAGWSCLLFLVFYWIIDVRGYRKWAFPFVVIGMNAVTVYMGRTLVPMTKIVGV
                     FTAGIAAGMGSIGHLFQAVAVLVVEWLILYWMYKRKIFLSA"
     gene            20914..>22527
                     /locus_tag="VLE22_09200"
     CDS             20914..>22527
                     /locus_tag="VLE22_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14624.1"
                     /translation="MISVASVYRLLLIWLACSCALTAKPYRFLLVVSDQWKDPASYII
                     EGGSDFAILAGLFKTWGLPFDVVRLDQQRLDKYHLLDRNGEPRYGTIVWSADPASLEG
                     KELGLIDVLVREYGVGLVALGDAVATPEISALTGLTYVSEYALIENPETVQEHFITRG
                     LKGREQEFLGGGGRRRGDKVTVQGATVLAKRGSLPFLAVREFDKGGRVAWLDAHRPSG
                     QISRQIMRDLLKRCLVWTQGYALYAEYSKSVVLEMHDMGASDKTFLPYWHYRTLSEEE
                     IRKAVIEPLKRHKAVLTQVVNTGFVDRKSQRVLNPWEQTRVVDELDGKTIHDYASTKR
                     GLDAGRREGVFEIQSHGWTHMLPDLDSPPGPFWNAPLDGIATLGFDTEFGDRMRNREI
                     PAVTQELHMRWSIEYLREDFGVTPLFIRPGGGEHSQTLANHTGRIAAGLGFGLTRLSS
                     PYYLGRDRVVALGPTVPQIGWAYNQKLSAGDMPWTIDGPVFIAFHDRDVAMDKGAIER
                     LLNDLGKGVRYMTAGEYCAYLHSTVARDPS"
BASE COUNT         4407 a         6841 c         6868 g         4411 t
ORIGIN      
        1 ccaccgagaa gtacctctgg tgatggcgca tcaccgtcac cagaacctct tcaggaagct
       61 cgaggtaccg tgcgtcgaaa cttccgagga tcggcgccgg ttgttcggtg aggtatacca
      121 gcgtgtcgag taacgcctca tccgccttca ccgttaagcc tctcccggcc agcagggctt
      181 caatctcgcc ggagattttc cgccgtcgcc ctgcggcgga cacgatcacg ccgttctgcc
      241 ggagctgctc ctcgtagttt gcgacggcaa ctcggacctt tttcttgccc aactgccggt
      301 gccccgcggt cgcatttccg gacttaacct cggccagttc gaaaggcacg accttgccac
      361 cgagcagtgc aacaatccag cggatcggtc gaatgaaacg tggaccgccc ttgccggtcc
      421 agtacatcgc cctcgggaag tggatcttca ggatcaggcc cggcagcgcc tccgagagga
      481 tctcggctgt ctgccgcccc cggaccttcc gctcataaga aagatactcg cccttcgctg
      541 tccggaccgt cttaagagcg gagacttcga caccgagctt gcgtgcgaac ccgttagcgg
      601 cgccctcgcc ggctgccttc ggcgggcctg tgaccctctc cttgctgtcc gcctgccgcg
      661 cgagaagccc gtcggctttc aacacgagcc gccggggcgt cgcatccacc caggtcacgt
      721 cgccggatag cttgttcgcg gccagcacgt cgcggaagag ttgacccagg tcttcgagcg
      781 ccggacggat catccagtcg gggatttctt ccgtgccgat ttcgagtagg aacggaagcg
      841 tcttcatgct gccgcctccg ccgcgttctg gtcgagccac gcctgcgcca cgccgaccgc
      901 cagcttccgc actctcccga ttacacccac ccgctcggtg acgctgatcg cgccgcgcga
      961 gtcaaggagg ttgaacaagt gcgagcactt cagcacgtgg tcgtaggcag gcagcaccgg
     1021 gaagcgcttc ttgtccgcgt agtcagggta ggcgtcgagc agccgccggc attccttctc
     1081 gtgcagatcg aacaactgcc acaacgtccc tacgtcggcc atctcgaagt tgtagacgga
     1141 gaattgaagc tcgtccgcca gccgcacatc gccgtaggag gcttgtggag cccactcgat
     1201 ctcgtacacg ctttccttgc cctggagaaa cgcagtgagc cgttccagac cgtatgtcag
     1261 ttcagccgga acgaggtcga gatccacacc gccgcactgc tggaagtatg tgaactgcgt
     1321 gacctccagc ccatcgagca tcacctgcca gccgatgccc catgcgccga gggtcggcga
     1381 ttcccagttg tcttcctcga acttgatgtc atgtttcccg aggtcgatgc cgatggcttc
     1441 gagactgtcc aggtactggt ccatcacgtc gtcgggcgac ggcttgagga tcacctgaag
     1501 ctgcgagtgc ttgtagaggc ggttcggatt ctcgccatac cgcccgtcgg ccggacggcg
     1561 gctgggttgg acgtacgcca cccggtacgg cttcggtccc agcacgcgaa ggaaggtctc
     1621 cgggcacatc gtcccggcgc ccacctcgac gtcatacggc tcctggatga cgcacccacg
     1681 cctcgcccag tagttcttca gcttgaaaag ggtctcttga aacgaatcca ttccggaact
     1741 tgcgctaacg tccaaccggc ggctcggcac agaacccttg tgctgcttct cggtacaggt
     1801 gcggccgctt cgtggcgggt tgactcaccc cggcggaaat gccggcggct ggcgtaagaa
     1861 tcaggttacc acaacgcggc ggcagaggct tttgtccgtg gtttcaccgt ggcttcacag
     1921 gctctcgagc agcggcgccg taatcagccg gcgctcgatg tgctcctcga tgcggcgaac
     1981 taggaagcgt cggagatcgg cggcggtctc ccgggtccac tgccgcgggg caagcgcgcc
     2041 gatcggcgtc gtcaggatct cagtggcaag ctccagtgag ctgtggctcg ctttcagctc
     2101 cggcaggaat cccgacagcc gcaccgacca gagggtgaag tagagcaccg ctgaccacac
     2161 tccttccgaa cgcaaggccc aaaagtgatc caatacggcg aggaggagcc ggaagaagcg
     2221 ctcgctgtgc tcagcaggag caaggagttg ctcggaaacc tcggccacgt agtcgagcgt
     2281 gacggccgcg gcgtaatccg actggaattc gaacgaagag cggatcagct cgcacgagtc
     2341 tagcgtgacc agctcgcggc tttccttttg gaagtaggag aggcggacgt gggagagccg
     2401 ctccaaaccc gcgccgaaag ggctcttcgg acgccgggca cgcttcgcca cgccccgcaa
     2461 cttgccgagg tcgcgggcca ggaagctgac gataagatcg ccctcctgga acgggtaagt
     2521 tcgaaggatc agcgactcgc tctcgcgggc aggcatctcg gcgaggacaa tgtagcacac
     2581 ccttgctata ctcggttttt tgaaccccat gtctacgccg aagtttccac tgcctgatca
     2641 tccggcgccg gtctccgagc tggtcgacac cctcagcgac ggcacgaaag tgaagcggcg
     2701 cgtcctgcgc cagcgcgcct gtaatgaaaa ggacgagaag ggaaaggtct gcgccgggca
     2761 cctcaagcgc tggtacttct tcggcgacga agtaaagcag aagttcgggt cggaggcgga
     2821 gatctaccgc tgcgagaact gcaagacgct ttaccttccc aacctcgagg aagaaccgcg
     2881 ctccggaacc ctccgctggt aggacgcctg cgcgggccgg cccaagaaag aaaaccgcac
     2941 cggaaggtcc ggagggggtt ggatgcctcc gcttccgatg cggtctgccc ttatgggctt
     3001 gggatccggc gccggcgcat ctggaaaatt gaacacccgc ttggccgttc cattcctggt
     3061 gaggcccgcg tcctatctct cctacaccac gaatgccaga tagcggccga aactcgagat
     3121 ccccggcgag acattatgat gccggtaatc caaaaaatga ccctctgttg caacctgcaa
     3181 tcgttggaat gagcggacta aactcgaaac accgtctccc gcgagcacgt cgttcgctga
     3241 caccacccat cgcccacgct tgcctgttgc agcagagggc aggttcaaag agctactcca
     3301 tcaattcggt cttcctaacc agcacccatc cagccaattg gaacaagctg taattaattg
     3361 aaaatactgg atattctcac aggtaagcaa ctctacagtc gtgtcactgc tgcgccgcct
     3421 gtgtcactgt gacacaatcg aatcgaatgc ccctccggag tgccccctgc ctcttgctga
     3481 ttctgatgct ggtagccacc gcctggccgc aggtcgtgga ggaggagtac cgggtctaca
     3541 cagagcaccc gaggctcttc ttgaacacca agcgtctccg gctgttgaaa cgggaacggg
     3601 agcgctcatc cctccgctgg cagcagtttg acgcgctgat gtccggcggc gcgcggatgc
     3661 ccgaacctgg gttcgcccat gccctttact cacaggtcac cggcgacccg gcgcactgcc
     3721 ggattgcgat cgactgggcg ctcgggccgg cggcagacct ccgccaagca gcgctggtgt
     3781 tcgattggtg tgagagccgt cttaccggcg acgaatcggc ccgtctcggg gccagaatac
     3841 ggacctggat cggtaaagcg gcgaccatgc acgaagtcgc ccccacgcga gatcgagcgc
     3901 tcgccgcgat cgcgctggcg gatcatgatt tcccagcttc ggaacagtac ctgcggggga
     3961 tcatccggaa atggtggcgt ggcgaaatgg cgcccgcact tcagtccgga aagaagaatc
     4021 tgaacagcga agacgtctac ccgctcctcg aaatcctgca tgccgtccag gacaacctga
     4081 aaattgatct ccgtgagaac gctcccgggt tctttcgcga ccttcccatc aaccgtctcc
     4141 tgagctacta tcccgcctcc taccccgcgc ccgagaatga gtaccgcatc cccgcgtgga
     4201 ccggcgacgg cgaaccggac ttggacgtag cggcgctctc ccgcgcggcc gacctcgcga
     4261 tgaccgctta cgatgcgaac atgcgagagc accagtttct ccagggctgg ctgatccacg
     4321 accgtttcct gttgcggagc gccttcggaa ctccttacga gttcctctgg gccaatcctt
     4381 accagccggg actgagctac taccacatgc cgctggcgca gcacgacacc cgccggggcc
     4441 tgctcttcct gcgatcaagc tgggacgacg acgcggtctg gcttggcgcc ttcgacggcg
     4501 agattcagtt gttcgagaac gggcagcgca aggtggtgaa ggcgggtccg ggcgccaaac
     4561 cggtgcgcat cggcgatgtc gtggccgtct tcgcctcagg cgcatctcgc ttcaacatca
     4621 acgacccgga ggcgaaagcc gtcttcatcg tcggactgaa gccgggcaag gcgtatgaga
     4681 tcgaggtgga cgacgaggaa atgcgggaga tatccgcgga tgctggcggc gttttggcga
     4741 tcgacatttt tccccggccg cgcctcggcc tccgccttcg tcccgcccct gacgcgagac
     4801 cctcctctta gtggccctgg atggtcatgc cgccgtccac caccaatgtg gccccggtga
     4861 tgaacgaggc ggcctcggaa gcgaggaaga gtaccgcctc ggcgatatcc cgtggggtgc
     4921 cgatccgtcc caacgggtgg cacttgagaa tgccgcgcat cgcctcgtga cgatcgggca
     4981 tggtcttaag ccaggcgtcc agcataggcg tgtcgatgta acccgggcag atcgcattga
     5041 cgcggatggc gtctttggcg tggtcaaggg ccatcgcccg cgtcatcccc aacatgccgg
     5101 ccttcgatgc gtcgtatgcc accacgccgg ggtacgacgc aaaggcatgt accgaggcga
     5161 tgttcacgat cgccccgccg cgcccgcgca ttttctccag ggcgtacttc gagcagagaa
     5221 aagcgcccct cagattgacg tcgagctggc ggtcccaccc cttcgccgag atctccgaag
     5281 cggagccgag cacttcgatt ccggcgttgt tgaccagtac gtccagacgg ccgaaccgtt
     5341 tcaccgtctc ttcaactgcc gcttccactt gttcttccac ggagacatcc gccgcgatcg
     5401 caagcgccgg ccctttcgag cctagttgtt cggccgcgtc ctgaatccgg tcggccagta
     5461 catccacagc agccaccgat gcgcccgcct ccagaaaacg ctcggcgatg ccgaagccga
     5521 tcccttgcgc tgcgccggtg actatcgcaa ctctgttgtc aaagcgcatg ggcggtcaat
     5581 aataccagtc ccgatttcga acttcgatcc ccgtcccggc caaccctttc tcgaacagca
     5641 gcaggtcaga gccgcggtaa tgatatgggt acacgacctt cggcttgaag gcgcgcacgg
     5701 cctcggcggc ctcctccggc ggcatcgtgt agggtaggtt catgcatacg aaggcgatat
     5761 cgacatcccg cagtgccttc atctcgggaa ttccctcggt gtcgccggaa acgtagattc
     5821 gcttcccgcc aaaagtgagc agatagccca cgcctcggcc cttttcgtga aacagcttgc
     5881 cctcggccgg tccgcgtttc aggttgtagg caggcatcgc ctccaccgtc cacttcccga
     5941 acgttttggt ctgaccggcc cggaccggca cggccccggg aatcttcgcg gccacaactt
     6001 ctggcccgac caccggtgtg cgcgccttct tcagtttgtc gaccatcgcc gcgtcaagat
     6061 ggtcgccgtg gttgtcggtg atcagaatga ggtcggcctg cgggaggccc tcgtagttgc
     6121 cctggctcca ggggtcgacg tgaaccacct gcccgccgcc ctccagcatc aggctcgcat
     6181 gccgaaccgg agtgatccga agcggtccct cagccgtcgg gaactcgtcc gtcttgcgct
     6241 tcgtttgtgc ggtgacaagt gcgcccagca ggaacagcaa tgcgacgctt ttcatgcctg
     6301 cattgtatcc gaacggcagg aactgcaacg ctacggcgga gttcctatac acgtagggga
     6361 tcggcccgcc actgcactca caacttcacg cgccgttcgt acttctccac cagcttgcgg
     6421 agatcgtagg tgaagtccac ccaggcctcc tcccacttcg ctgccccagc cttgtcgtat
     6481 ttgtaaaagc ccttacagtt ggagatgccc tgggagccac ggccgacgac gtctttcatc
     6541 aactgcggca cttcgctggc gttgcacaac tcgggcatca ggccttccat gactgtccca
     6601 taagcgggta ttccggtcag gtccatccac cggaacgggc cggcgatagt ggcccaccag
     6661 ccgatgtcgt tacgaaacga gcgatcgaca tcctcgaggc tggcgattcc atgctcgacc
     6721 aggtggaacg cttcgcgcag catggcgtac atcatgcggt ttgagaggaa gccccgaatg
     6781 tcggtgttca gcaccgtcgg atccttgccg caagcgattc cgatcttacg cgcgaggcga
     6841 acagcccgcg gcgccgtggc ttgccccggg atgatttcca ggtaccgcat gacctgcacc
     6901 ggttcgcccc agtgcattcc gatgaaccgc tccggccggc ggcgtccctc ctgaagcagc
     6961 gtgatgggaa tgctggaagt gttcgacgag atgacagtgc tggccttcac agcagcctcg
     7021 agttcgtcgt agatccggcg cttcagttcc acatcttctt tgaccgtttc gacgacaaag
     7081 ccgcagggag cgagttgtcc gacggattcc gcgagttcat acctatcgcg ccaaccccgc
     7141 acttgcgccg gtgtccgcag gccgcgcttc accatctcac gaagggccgc ggaaacatgg
     7201 tcgatgcttg ccttggcccg gctcgccgtc cggttgtagg cgatcacgcg gaagccataa
     7261 gccagcagac acgaagcaat accctggccc atgaggccca agccgatcag cccgacggat
     7321 ccggcatcga ctgttttacg aggttttcca agcgttgcgt cctttgctaa tcccacgggt
     7381 gttctcccct cacgttgtcc acgatgatct gatacagact ttgctgatat gcgaaaagcg
     7441 gtaacttctc ctcaacttca aagatgtagc tcagatccat cgtcttgttg ccgtaataga
     7501 agtcgcgatc gatttgatgc acgaatttgt ccctgcctcc agggacatga cgaaagtcct
     7561 tcgggtaggt cgatttctcc cagacctgca ctgtctcgtc gaacagcgcc gacttctcat
     7621 ggcgcaggtc gtcgatgatt cgaccgatcc ggtgatagcg attgatggcg gcctggtcgt
     7681 cacccttctg gtgatcgtgc atggccgcct gaagattctc gtcgatctcg ccgatgctca
     7741 gcaccagttt cgtgaaatga agcatggtgc gggcacacaa caaaagcact tcgaggttat
     7801 atctgttttc cggatctcgc gccaggttcg ccttgatgat ttcggtcgcg cgttcatatc
     7861 ggggtagccg ctgctgcgcc tgcactagta gagaggcatg cttttgcccc catgaggtgt
     7921 cgacccgaaa ggcagcatcc ggaagcggcg gaagagccaa tgccccgaat tcccttgtcc
     7981 agccactttc tctgcggatg aatcctttct ccgagagaat gtcatacgct tcggccagct
     8041 ccgtctgcct ggggccgtag aacagaggaa agaacttggc ttcgaactct tgcgtcgtgg
     8101 gcttcttccc ggaccaggag tactcggcgg aacagacgaa tccgatccag tacgtctcgg
     8161 ggtggattcc ctcgtcttcc catgcggtgt tcaggactcc cttgactccg agcgcgaccg
     8221 cgtccggaat gaagttggcg ttgttgtaga cacgaagacg ctgatcggga aagatggggg
     8281 tcatggtttg cgccgcggtc gcgacccaaa ccggatactt gagattcagg atcagtttgt
     8341 cgcggtcgac gaacttaccg gcgtcgtagt cccagcgcat gtagatgagg ttgtcgggga
     8401 ggcgtgccat gtcggcggca ttgaaatgcc gcaggaacat gtcatcccac acgatggcgg
     8461 tcttgccccg cttcttcagg agatcgcaga cgaaggtgag ccagagcttg tagctttcga
     8521 caggcgcctt gtcgccccac ttcgcacggc attccgggcc catacccaac tcacgcgcct
     8581 cgtccccgcc gatatgcacg tgcttagccg gctcgacgac atcgagcagt tcgtcgagca
     8641 tctcccggat caggccgaag gtcccttctt tcagcgggca aagctgccag ttgctcttag
     8701 gtacttcgcg caggtgttgg aactcagggt gcttcgcgat gaagctgacg tggcccaggg
     8761 tctggaccag aggcacgatc tcgatgtgat atttcgtcgc agtctgcaca agctgcctca
     8821 cctgctcgtg agtcaacgct cccggagccg acagaacagg gtgtcgtgtg tactgaaact
     8881 tatcttcaaa ctcgaagacg gcggtgttga ttttgtatcg cgccaactgc cggaacaggt
     8941 gttcgtaata ccagggccgg tccaggtggt accagaggtc gacgtggatg gcgcggaact
     9001 ccatgtcggg ccagtcgagg atgcgaagcc cgcgtatgtt gcccttcccc tgctgaagca
     9061 gttgcaggag cgtctggaca ccatagaaca acccttgcgg gaatgccgcc tcgatggaga
     9121 cacctttctg tgtcacagcg agaatgtacg cttcctttcg cgcgctcccg ggcggcacgc
     9181 cttcgaggcg gctctccgga acgatgctca gaatgatccc gttcgagtcc ggcctggaac
     9241 cggccgttcg gagccagagt ccccttgtct gctctacgcc ctcgatcagg ctggccaccg
     9301 ctgaggagtc ctgtccgccg ggagtttgat agacggccgt gttggcgtcc ggctggaagc
     9361 tgtcttccgt gactttgacg atttggggct cgggaatgat cacaatcccg tagcgaccgt
     9421 gtacacccgg ctgcttgggg atttcttcgg cagccggatt cgcgaggaac agcgacgaaa
     9481 tcagaaccga cagaacgaga atgcgacagg tcatcgggat acctccgcgg acacaccagc
     9541 ccgcctgagc gggtataagc ttcttgattt tctggaccat cgaaacttct gtcaacggca
     9601 gaatcgcttt tgtaccggag accgttcaga catctggacg cgacgcatcg atccagcgct
     9661 ctggacggtc cgcggtgcag tatactctgc ttcaaatgga aaacattgtt ctgggaaacg
     9721 gcacactgat tgacgggacg ggcagccggc cgaccacggg agacgtgctg atccgcggtg
     9781 accggatcgc cgaagtgggc cgattcgaaa aaccggcaga agcccggctg atcgattgct
     9841 ccgggctgac ggtggcgccc ggcttcattg acgcgcacag ccactccgat cttcaggttc
     9901 tcgagaatcg ggaggagaag gtccggcagg gggtgaccac ggaggtggtc ggaaattgcg
     9961 gtttctcccc gtatcccgcg ccggatgacc gcagcctgct acagggtttc gccaatggga
    10021 tcttctgcgg tgacgatcga tggggctgga gatcggcccg cgattacctg aaggatgtgg
    10081 agcgaagccc gatcgtcaac gtcgcttcac tcgtcggtca cggcagcctg cgaatctcgg
    10141 tcgccgggca ccggctgggt cctctcccgg agcgcgacgt ggaggcaatg gaagagaaac
    10201 tatcggattc tctgtccgag ggagccagcg gattgtccac cggcctgatg tatgcgccgg
    10261 gatcgagcgc gcctttcgaa gaattggagc gcctgtgccg ggttgtggcc aagcacgaca
    10321 agatctatac cacgcacatg cggagctact tctccggact ggtggacgcg ctggacgagc
    10381 aactcgatct ggccaggcgc acggggtgcc gcctccagat ctctcacctg caagctgtcg
    10441 gcgcgagcaa ctggccgttg caggcccggg ccatcgacaa aatcgagcaa gcacgccggg
    10501 acgggatcga catcgccttc gactgctacc cctacgttgc cggaagcacg gtgctgacgc
    10561 aggcgctgcc gcagtgggtg ttggaaggcg gagttgtggg aatgctcgcc cgcctgacgg
    10621 acccctcgca acgtgcgcac atcgcagcgg agacgaagtc ggcgctggaa tggcgctggg
    10681 tggacatcta catctcggcc gtcgggtcgg cggccaatca ggaggcggtt gggcagaatc
    10741 tgacggcgct cgcggagacg cgcgggtgtg agccggtgga tgttatgctc gacttgctga
    10801 tcgaagagcg tggagcggtg aacatgctct gcttcaatca gagcgaggag aacctgaagc
    10861 agaccctcac gcatccgctg tcgatgatca tcagcgacgg gttctacgtg aaaggacgcc
    10921 cgcaccctcg ccttcacggg acatttcccc tgtggctcgg tgacatctgc cgcgaaaggc
    10981 gctggctcga actcccagag gccgtgcaca agatcaccga ccagccggcc cgaagattcg
    11041 gcataaagaa ccgcggacgg ttgttgcccg gatacttcgc ggacgtcacg gtgttcgatc
    11101 ccaagaacgt tgggagccct gcaacatacg actcgcccga gcaggctccc atcggcatcc
    11161 accacgtttt acggaacggt aagtcgcaat tcggcggcgg ggcagtgtga gaagcgcctc
    11221 agcgctctag cgcacttttc cccttccggc cacttcccac tcggccacta catcttcatg
    11281 ctggacgccg tagacgcgat catgcatatt gacgcacgtg cagacgtgat tcggaactac
    11341 gcgcacacgg tcgccgatct tcaggcgggc gccccccagt ctcaggtgtc cgtgttcctc
    11401 cgacagatcc tccacgagga tttccggatg cccgatcacg tggccgaacc cttgccggtc
    11461 accgcttaga agagagtcgc cggaaaaagt cttgccgccc gcgtcgatga tcgcttttcc
    11521 agagacactg gtgctcacaa ccgtggcgag gacagtgacc gcgcagtcgt cccatgaggc
    11581 agcctcggcg cagaccgtgt tcttgtcatt gaagatgtat gtgcccggcc ggatttcgtt
    11641 gattcccacg aaacggtgcg acaaaaaagc agccggcgtg ttgcctccgc tgacgatcgg
    11701 gcagtcgatt ccctctgact tcagcagctc catcagacgc gagagtgtct cgttctcccg
    11761 tttcagcagc acctctcgtt ccgatgcaga gcccatgata tggcccgggt agacgagcac
    11821 cccacgccat tcgagcctcg gaagctcccg gattttccgg acggcaggca acgaagactc
    11881 gtcgatgggc agcccacacc tgccaaaacc ggcgtcgaac tccacgagca ggccgacggt
    11941 aacacagtcc tgccgcgcct gttccgatat cccgttcgcg atctccacgg aatcgcacgc
    12001 gacagttatg tccgcgcgtc gagcgagggc cgccaggcgc cgccatttcg cgccgccgaa
    12061 cagcgggtac acgatcagaa tgccgggaag cccggcgtcg gccatcactt ccgcttcgcc
    12121 gagcttggcc acagtgatgc cggccgcccc gtgatcgatc tggcgccgcg ccagttcggg
    12181 tatcttgtgg gtcttcgtgt gcggtctcaa tcgaaggctg tactgcgcgc agtagtctcc
    12241 caaacgccgg agatttctct ccatgacatc gacgtcaacg agcaccgccg gtgtatccag
    12301 atcggtaata cgcatttgga actctggtag cgatgataca acttcctcga tttctggtag
    12361 aattttcaac agttccgagc cacctggccg ggggcgacat ggcggcgagc aaaactccaa
    12421 cagtgtcatc cgtggagagg acccttcata tcctggagtt gctggcgacg tctcgagccg
    12481 gattgacttt gccggagatc agccggcggc tcgggctgcc gaagagttcc gcccattgcc
    12541 tgcttctcac gcttgagcgc agcggctatc tcacaagcaa tacgacgacg cgccgcttca
    12601 tgttcggatt gaagcttttc agcttggcca acatggcgca cagcggcgtg cggttgcgtg
    12661 agcaggcccg gcccttcctg cgttcgctgg tgaacgagac cggcctgacg gtccacatgg
    12721 gcattcggga gcgtgacgag gttgtgctgg tcgagaagat cgggcccgca gccgcgccga
    12781 agctggcgac gtggctgggc aaacggatgg acctgcattg cacaggggtg ggcaaggccg
    12841 tgctcgctca cctgccggct gaggaattgg agtccttcat cagggcgcgc gggttgccga
    12901 gacacaacga aaatacgatc gcttcgattc gccgtctgcg cgagaaactg gctgaagtcc
    12961 gcagcaccgg ctatgcgctg gacgacgaag aggacgagat cggcacgcga tgcatcggcg
    13021 ctccaatctt tgacccggcg ggcacagcga tcgccgcggt cagcctggct ggaactacgg
    13081 atcagattac ttcggataac ctcggacact tcgccgggtt ggtgaagcaa accgcaggag
    13141 ccatttccca cacgttgcgt ccggaggaga gcccggcttc agaagagccg tccgagccgc
    13201 cggctcaaga acgcgagaac tcgattgctc aggggatctg agaacggccc cccaaagagc
    13261 cagaacctct agcgggcacg atctccgata tcctcgggat acttgagtgc cagtgcgcgg
    13321 atatcgtagc tgaacttcag gaacagtctt tcccagcgtt tcgcctgtgc gggcgtgtat
    13381 cggtagaagc cttttgcgtt tgcgactccg agtgcgccgg agtctacaac cttcttcatc
    13441 agcctgggga ctttcgttga gcagtcaagc tccggccaaa ggtctcgcat caccgtacgg
    13501 taggcgggga ttccggtgag atccatgaac cgaaacggtc ccgcgaatgt aatccagtag
    13561 ccgaggtcgt tccgcaacga ccggtctacg tcctcgattg tggcgtagcc tgactcgacc
    13621 aggtggaacg cttcccggat catggcgtag aaacagcggt tcgtaatgaa gccgcggacg
    13681 tcacggcgca gcagtgacgg ttctttgccc caccggtgcg ccagggccat cgcgtgtgcc
    13741 gcgctcgcgg gacgggtctg tttgccgcag atgatctcca tgaaccgcgt aacgtgggca
    13801 ggctccgccc agtgaaatcc caagatgcgt tccgggtgca gagccccttc ctgaagctgc
    13861 gtgacgggaa tcgccgatgt attggtgccc aacaacgctc ttcgtgagac gacctgctcg
    13921 acgtttcgga tgatctcctt cttggccttg acactctcaa tgattgactc aattacgacg
    13981 ccggccttcc ggagggccga gaaatcggtg gaaatcgtca ggcgttcggc gacggctgaa
    14041 gggtcttcct tgagtatccc ttcctcctgc aactccttga gcaaagcaag tacctggcgc
    14101 cttgtggtct tgagcttgga ttgtgtgccg gtaatgccca caaccggatg tcccgaggcc
    14161 agaaggcacg tgacgatgct cctccccatc agccccaagc ccacgacgcc tgccgtgatt
    14221 tcggaggggt cgatatttcc gcgcgatttg ttccgtttgc ccatgtttat tctgaccctg
    14281 tagtggattt cagacttcgc cctgagcgga atgttcccac agagacgctt gagcaggcaa
    14341 gcgtgaacag ggccgctgcc attctggcgt tcaacatcct ggacagtggc gggcagatgg
    14401 cccagaattg tcttgacgat cagggaggcg caggtaaact gttgtaaacg aacagcctat
    14461 gtttctcccg gcagcccttc tactcctccc aagtgtcggt cttgccgcgg cccctctcat
    14521 ctatccggaa ccgcagcaga tgagcattgc cggcgggcga ttcgaactcg acgaaaaggt
    14581 atcggtcgtg atcccgcagg cagcctcgga ccgcgatttg tcgctagccg ggttcctcat
    14641 cgcggaattg agtgaccggt acgggctggc gcttcagacc gagcgggtca ctgcagtgcc
    14701 cgcctcgggg cgcgtgatct tgatgggatc ggtcgaaaac cccctgatca gaaaatactg
    14761 cgctcgacag aaccttacag tgagccggga cagtcccggc ccggagggct acgttctgca
    14821 cgtcggtgaa cgggtcgtgc tggtcgcggg tagtgacgat caaggcgcat tttacggcgt
    14881 gcaatccctg cgccagctag tggaacgcgg gcagggccgg agtatccagg ctgtccaagt
    14941 tcgcgactgg ccgcacaagt ccttccgcgg catcaaactt tatctgcccg ggcgcgagaa
    15001 ctttgctttt ttaaagcggt tcctgcggga tttcatggcc ctctacaagt acaacaagct
    15061 catcgtcgaa ttgaacgcgg cgatgaggct ggatcgtcac cccgagctca acaccggctg
    15121 ggctgagatg gccaaggatc tcatttacag ccgccgcgac cgctccacag ggccaaacgg
    15181 ggagtatcag gactccacac atcacgacac gggagacggc ggaattctgg agaaggctga
    15241 agtggccgac ctggtgcgct gggccactga gtacaacatc gaggtcattc ccgaactccc
    15301 ctccttgacg cacagctact accttctgac gagccaccgg gagcttgccg aaatgccgga
    15361 tgcggagtgg ccggatacgt actgcccgtc gaatccaaag agctacgagc tgctgttcga
    15421 tgtattcgac gaatacatcg acgtgatgaa accgaagctg attcatgtcg ggcacgacga
    15481 atggagaatg ccgtggggcg tgtgtccgaa atgcaagggg aaggaccaca gggagctgta
    15541 cgcccaggat gtcatcaaga tccactccta cctcgcaagg aagggtgtga ggacggcgat
    15601 gtggggcgat catcttctgg aggccgtggc cggcccgatg ctagtcgacc ggaagacccg
    15661 cgcgggatac aagtatcgaa ttcccggcgg gcttacgccg gatcaggtgc agaagctgat
    15721 ccccaaagac atactggtat ttaactggtt ctggaatgac tcggagcgcg gttctggaga
    15781 aacgaacgag gagacgctcg ggaagtgggg gttcgaacag gtctacggca atttcacgcc
    15841 cgagatcagg aactacggtc gccgcagcgg tcgctccggg atacttggcg gcgcgccttc
    15901 ggcgtggatc gcaacgacgg agtttaactt cggcaaggac ttgatctacg actttgcggg
    15961 ctgcgcgagc ctgctctggt cgaaacattg gccggaggcg gagcagttgt cacgcatcgt
    16021 ccagaacatg atgccgcaaa tccggcgaaa cctgagcggc cgtgcgactc cgagtgaaga
    16081 cggggaggta gttcgcatgc tgccggttct ctctttgcag gcgagttcgg caaaggcggc
    16141 cgaggcggtg gacacggctc aggtgaagag tgggagggtg gcatcgggac gggccacgtt
    16201 cgaactcccc ggaaaatcga tggtggcgca ggctagcgac ggaattccgg tcggcgagga
    16261 tgccagcagc ctcatcttcc tgcacgctct ggcaaagccg gccagcaacg agccggggca
    16321 ccgctacatt tacaacttcc cggacacggc cgacctgctc ggttggtacg aggtagttta
    16381 cgacgacggc ttcgtggaaa tcgttcctgt tcgctacgga gagaacatac tcgagtggag
    16441 ctgggggcgg cgtcatcaac cgtccacata ctgctaccgt gccgacccgg tggatatcgg
    16501 cagagcagcg gggaacccag tcacgttctt tgcgttcgag tggatcaatc cccggtttgg
    16561 caagaccatc cgtgaagtcc gtttgaaggg gacgaccgga ttcaggaata cgcggggtag
    16621 ggttattccc gacaactcca tcgtcctggc ggcaatcagt tcaacgaaaa agagaggcta
    16681 tcccgagccg gtcaaggctc gaacctacgt gcggcaagaa tccccatgaa tcccggcggt
    16741 ccgggtcttg ggcgaagagg aggatgagat gagcgaaccg gataggagac gatttctgct
    16801 gggtgtggcg gcattgggag cgggttcgct tcacgcgctc gaaaccagcg aaatcagaac
    16861 ggcatttatc ggcgtcggga accgcggcaa ggagctactc cggcaggtgc tgaaccaggc
    16921 gagtgcgccg gtggctgcga tctgcgacac gaatcccacc gcccgcgacg aagcgcaggg
    16981 ccttgccagc cgctacagtc cacgatctct caccgagtgg cgcagagtcg tcgacatgaa
    17041 ggatgtcgat gcggtcatga tcgcgacgcc ctgcaacctg cacgccgaga tggcggccgc
    17101 ttgcctggac gccggaaagt atgtctattg tgagaagccc ctcggcatca ccgcggagga
    17161 ggtggaccgt gtggtgaagg ccgcccggcg ttccaaagcg tttctccaga tcggccagca
    17221 gatgcgctac tacccgtggc tccagggcgc gatcccgcat atccacaacg ggctcatcgg
    17281 caagccgctc atcatcaaag cgcaacgaca cagcacggcc accccagaac aggaaaaggg
    17341 gcacctccct gagtggtact tcgatccgag gatttctggc gaccgcatcg tcgagaacgg
    17401 ggtccataat ctcgacgtct gcaactggat tgcgggtagt ctccccgtca gcgtctacgg
    17461 cttcggaaaa acctacctcc cgaaacccaa gcctcccggc gaggagtaca tggacgcata
    17521 cagcgtcatg tacatgtacg agaacgaggt caaccttacg tacagtgaag cgaagctaca
    17581 cccgcgcgcg ctcaaggagc tggcaaacgg tcagtggtac gtgatcttcg gcgagaaggg
    17641 cgcggtgacg atcaaacggg gctcggcgct gttctatgag atgtcctccg aatcggagcc
    17701 tcgcgaattg cttacggcgg aacagcaaaa cgcgaaggaa gacgccatgg gggatttctt
    17761 cgcttcgatt cgcgagaaga agcagccact cgccggcctg cgggtcgccg cgaccgcggc
    17821 gctcacgacg atcatgggcc gcaaagctat ctatgagaga cggatggtca cctggaagga
    17881 actgggtgtc tccatctgac gaagaacagg agagtatcaa ggatgaacag gttgttttcc
    17941 tcgatgagcc gcctggctgc gggcttcgtg cttgtagtct cttgcctgtc gctcactgcc
    18001 caggccgcct ctgagcgtcc gccgctgccc tatcgattcc tcctggtcat ttccaatcag
    18061 tggaaggatc cggcgagcat cttggtcgag ggaggaggcg agtttcaggc ggtcaccagt
    18121 ctgcttaaga catgggggct gccgttcgac atcctgcgtc ttgatcagca accgctggat
    18181 cgctaccacc tgatcgatcg ggagggtcac ccgctgtacg ggaccatcat cttcgatcct
    18241 gaccccgcgg gcctggaggg taaagacctg ggcttcctgc cggctctggt caaggatttc
    18301 ggtgtgaaca tggtggtcct cggcgacgcg atatcggcgc ccgaagctgc tgccctcgcc
    18361 ggcctggaat acgtcagcga ataccgtcta ggggacggaa tgacattcgc aggcgagcac
    18421 ttcatcaccc gtgggctcgc tggaagagaa aaagaactgc ttcagggagc gggctgggag
    18481 atatcgggca gcaaggtcgt ccccaaagat gcgacggtgc tggcgaagcg agcccacctg
    18541 ccgtttctca ccgctcgcga gactccgggc gggggccgcg tggcatggct gggcgctcat
    18601 cgcggcagcg tgcaaatcgc caagcaggtc acgcgggatc tgttcaagcg gtctctcgtc
    18661 tggacgcaag gctacgctct ctacgccgag tatccgaaga gcatgatttt gttcatggac
    18721 gatttcggaa catcggacag gtcgtacttg ccctactggc actacaagac tctcgatgag
    18781 caggacatcc gcaaagggct catcgagccc ctgaaacggc accacgctgt aatcaaccag
    18841 gatgtgctta ccggctatat cgaccggaaa gggcgccgga tcgctaatcc ttggaaacag
    18901 cgggtgatcg acgagatcga cggcaagact gtgcatgact acgtctccgc caagcgcgga
    18961 ctcgatgccg gccttcggga gggcgtgttc gaggtccagt ctcacggata cacgcacatg
    19021 ttgccggacc tggagtctcc tcccgggccc ttttggaccg cgccgatgga cgggatcggc
    19081 accctgggct gggatgtgga attcggcgac gagctgagga agcgcgaggt cccggccatc
    19141 acacagcggt ttttcctcag ccgcggactg gcgcatatcc gtcaggactt cggcgtgacc
    19201 ccgctgttcg tcatcaacgg cggaagcgcc tggtcccatt cgtacaccaa caacagcccg
    19261 aggattgccg cggagatggg atttggcttg tcgatctttg gaaagcccgg atacctgggc
    19321 agagacctgg tgatcgcgtc catggagccg atcgtcctga acggaggttg ggcgcacgac
    19381 cgcaaattga ctggtgatga catctcgtgg accattgatg cgccgtactt cctcgtcttt
    19441 cacgaccgcg acatctcgat cgatccgacc gcggccgaac gcctgctgac gggcctcggc
    19501 aagggagtcc gttacctgtc agccaacgaa tacgccggct atctccacgc aaaaatcgaa
    19561 cgggataaca gttccgggca atcgctggca ctcgcagtgg attacgacga acattactgc
    19621 cggtacttcg cgacgcatga atccacgtgg gttcttcact tgtcggacga aatgcgcagc
    19681 ggactgaggg ctacagccga gaagcaggtc atcacaatcc cgaagggtct ggggcggcac
    19741 gtcgttaccg ccgcagggac ttcgcaatag gaggttaccg tatggaaacg gcagcgacgt
    19801 ctcaggaacc cggacaggcg gctgcctcgg cgccggcggc ctcgaaacgt ctggtttccc
    19861 tcgacgcgtt tcgcggttgg acgatgtttt ggatcgtcgg cggcggagca ctggtgaccg
    19921 gtttggaggc gctgggccac aaccctgtca tcgatacgct tgtgtatcaa ctggagcaca
    19981 cgccgtggca gggactcagg tattacgacc tgatctggcc gtcattcatg ctgatgaccg
    20041 gcatgtctct gccgttctcg tatgcaaagc ggtcgctgac gcagacgcac cgcgaaatcc
    20101 tgctccgcgt gctgcgccgc gtgatcgtgc tgctcctgct cggctccatc cgggagtcgg
    20161 tgagcctgaa ccatccgtat tgggtggagc tgagcagcgc attgcagcca attgccatcg
    20221 cctacttctg tgcgttcctg ctggtgcgca agtcctggcg cttccaagcc gccgttggcg
    20281 cgtcgattct ggccggctat gcggtattgc tggcctttgt tccggcgccc ggcatcccgg
    20341 ccggcagcta cgagaacggc aggaacctcg tcgccgccgt cgatgtcgcg gtgctcggcc
    20401 ggacgcacaa ggaaggttgg ggaaccgtaa tcagcaccat acccacaatc gcgaccacaa
    20461 tcctcggctt gctgatcgga gagcttctga gaagcacgcg tccgcagaaa gccaagctcc
    20521 ggattatggc gctggtcggt atcgcgggtg tggtgctggg tttcgccctg gatcccgtga
    20581 ttccgattgt gatgaagctc tggacgacat cgtatggtct ggcctccgcc ggatggtcat
    20641 gcctcctttt tctggtgttc tactggatca tcgatgttcg cggataccgg aagtgggcgt
    20701 ttccgttcgt tgtaatcggg atgaatgcgg tcacggtgta catgggacgg acgctcgtgc
    20761 caatgacgaa gatcgtcggc gtgtttaccg ccgggatcgc ggcaggtatg ggctccattg
    20821 gtcacctgtt ccaggcggta gcggttctcg tcgtcgagtg gctgattctg tattggatgt
    20881 acaaacgaaa gatcttcctg tcggcgtaag agaatgattt ccgttgcgtc tgtgtaccgt
    20941 ctgctcctga tctggctggc gtgcagttgc gcgctgacgg ccaaaccata ccgtttcctc
    21001 cttgtggttt ccgatcagtg gaaggatccg gccagctaca tcatcgaagg aggcagcgat
    21061 tttgcaatcc tcgcaggact gttcaagacc tgggggcttc cattcgacgt tgtccgactc
    21121 gatcagcagc ggctggacaa gtaccatctg ctcgacagaa acggtgaacc ccggtacggc
    21181 acgatcgtct ggagcgctga cccggccagc ctggaaggga aggagctcgg actgattgac
    21241 gtgctggttc gagagtacgg cgtggggctc gtggctctgg gagatgcggt ggcaacaccg
    21301 gagatttccg ccctcaccgg tctcacctac gtgagcgaat acgcgctgat cgagaatccg
    21361 gaaactgtgc aggaacattt catcacacgt ggcctgaaag gccgtgaaca ggagttcctc
    21421 ggcggaggag gccgtaggcg cggggacaag gtgacggtac aaggcgcgac tgtcctcgcg
    21481 aaacgcggtt cccttccgtt tcttgcggtt cgtgagtttg acaagggcgg acgggtagcc
    21541 tggcttgacg cccaccggcc cagcggccag atcagcaggc agatcatgcg cgatcttctc
    21601 aagcgctgcc ttgtatggac gcagggatac gctctctatg cggaatactc gaagagcgtg
    21661 gttctcgaga tgcacgatat gggggcgtcc gacaagacat ttctgcccta ctggcactac
    21721 cggacgttga gtgaggagga gatccggaag gcggttatcg agcctctgaa gcggcacaaa
    21781 gccgtgctca cacaggttgt caacaccggc tttgtggatc ggaagtctca gcgggtgttg
    21841 aatccctggg aacagacaag ggtcgtggac gaactggacg gaaagacaat ccacgactat
    21901 gcgtcgacaa aacgagggct ggatgcgggc cggcgggaag gcgtgtttga gattcagagt
    21961 cacggctgga cccacatgct gcccgatctg gattctcctc ccggtccttt ctggaacgcg
    22021 cctctggacg gaatagcaac cttgggattc gacaccgagt tcggtgaccg catgcgcaat
    22081 cgcgagatcc cagcggtgac gcaggaactt catatgaggt ggtcgatcga gtaccttcgg
    22141 gaagacttcg gcgtgactcc cctctttatc cggccgggcg gcggggaaca ttcccaaacg
    22201 ttggccaacc ataccggacg aatcgccgcc gggttgggat tcggactgac gcggctgagc
    22261 tcaccgtact acctggggcg cgaccgcgtc gtcgcgctag gtccaacggt cccccagatc
    22321 ggttgggcct acaaccagaa gctctccgcg ggcgacatgc cgtggacgat cgatgggccg
    22381 gttttcatcg cgtttcacga tcgggatgtc gcaatggaca agggcgcgat agagcgtctg
    22441 ctgaacgatc tcggcaaggg cgtgcgatac atgactgccg gcgaatactg cgcgtatctg
    22501 cactccacag tcgcccggga cccgtct
//
LOCUS       DATIFQ010000199        14530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2171958, whole genome shotgun sequence.
ACCESSION   DATIFQ010000199 DATIFQ010000000
VERSION     DATIFQ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14530
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171958"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..559
                     /locus_tag="VLE22_09205"
     CDS             125..559
                     /locus_tag="VLE22_09205"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HSB14625.1"
                     /translation="MKEQNYTNHRQYVFMYHVVLFGLIVLTLIGSFVNLYKSIGDHQR
                     LYSASLIVVMNICLLLLFFLARVFALKAQDRAIRAEENLRHFVLTGRPLDRRLTIRQV
                     IGLRFAADEEFAELAKRAVTESLSEDAIKRAVKSWRADTHRV"
     gene            complement(566..2116)
                     /locus_tag="VLE22_09210"
     CDS             complement(566..2116)
                     /locus_tag="VLE22_09210"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB14626.1"
                     /translation="MKTKPSTYRRPVSRRELFRGSGIAATAGLMGAAPAAGKEPAPDV
                     YTRLGVQPFINCTATYTINGGSRLLPEVIEAIEQASHYHVNLDELMEAAGRRISQLLR
                     VPWAMVSSGAAAALSHATAACVAGADPERMKQLPDTAGLKSEVILPRSSRNEYFHAVR
                     AVGVRFVEVDSPEQMRAAIGPRTAMALVLGNRFENVSLPLKEVSEIAHQAGVPVLVDA
                     AADYLIVPNPYIADGADLVAYSGGKILRGPQTAGLLLGREDLVRAAYANSSPHHAFGR
                     MMKVSKEEIVGMVTAVESWVSRRNRETEYKEWESWYAHIASRLKAVDGVSTRVLPPSR
                     GGPFPILEIGWDPASIGLTAGELHDLLLKGEPAILTHAEGEGHSFVLRPVAMRPGDYR
                     IVANRLFQLLHSAPSPKTSVPKPAASDISGRWDVSIRYLRGEARHTLDLEARGDEVTG
                     RHTGSRLRGDVKGTVQGDQVRLRSVLPYEESQLVYVFEGTLSSGRMEGELLLGEFPKA
                     RWTAVRTS"
     gene            complement(2170..2619)
                     /locus_tag="VLE22_09215"
     CDS             complement(2170..2619)
                     /locus_tag="VLE22_09215"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSB14627.1"
                     /translation="MTKNRRTFLRSAAGLGTGGAMAAMANAAAPEGRIKAPGGYRVGN
                     LYFSSGLTGVRPEAQKDPLAFGGDVKEQTRRTLDAHKKNLEAMGSSLENVIKVTAFLA
                     DVKTEKPAFNEAYAEYFKKGAPARSAFGVEFPDSATRVEIELIAWVP"
     gene            complement(2693..4120)
                     /locus_tag="VLE22_09220"
     CDS             complement(2693..4120)
                     /locus_tag="VLE22_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HSB14628.1"
                     /translation="MLTRRTFLSLPAAIAAAEPAEKPNIVVVLCDDLGYGDIACYNDE
                     SRIRTPHIDRLASDGVRFTDAHSPSAVCTPTRYGLMTGRYAWRTRLTHGVFHGTSPAL
                     IEDGRETLTSLLKRSGYRTGCFGKWHLGLGTREPADYSRELRPSPLDYGFDEFFGIPA
                     SLDMPPYLYIDGRRAVEQPTSTISDSGASPRGPFWRGGAIAPGFRMDEVLPKITERAV
                     RFVRESLQPFFAYVPFTAPHTPWVPAAAWRGKSKAGQYGDFVAQVDASTGQLIETISR
                     RGVARDTLFIFTSDNGAPWSEPDIQASGGHRANRPWRGQKSDIQEAGHRVPFVALWPR
                     HIRAGSLSSQTLCLTDLFATFAAVTGRTLGRDDAEDSYNMLPALLGKANRPIRHAIVS
                     HSGQGLFSIRSGSWKLVLGRGSGGFTKPDRIEPGPGEPAGELYNLAGDPHEDRNLYQQ
                     HPEKVEELSALLERYRRQGYSRPMA"
     gene            complement(4169..4265)
                     /locus_tag="VLE22_09225"
     tRNA            complement(4169..4265)
                     /locus_tag="VLE22_09225"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4229..4231),aa:Sec,seq:tca)
     gene            4576..5946
                     /gene="dnaB"
                     /locus_tag="VLE22_09230"
     CDS             4576..5946
                     /gene="dnaB"
                     /locus_tag="VLE22_09230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSB14629.1"
                     /translation="MATTDLALQKGLPANTDAERFVLGSILLDDAAYVQVASALDASD
                     FSLEKHRRIFLRMAELHEHGEKIDRVTVANELIKHNQLESCDGLGYLVSLDDGLPQIY
                     NLDSYVRIVKDKSLLRRLIFTSQKLIDRCMMGDEEPTEILASAEETLLKLGETTTKVG
                     LATPAEVIEGYEGGLNTFLDPGKRIRGLSTGFKKFDEMTGGLHPGELIILAGRPSMGK
                     TALALNIAQHAAVKLGKTVAVFSLEMSNESLLTRLLCAGARVDSHRFRLGYLDGDERR
                     RLQAAAMELVEAPLFIDDTSGSNLIDMHAKLRRLKAERQLDLVVVDYLQLMSGRGRFD
                     SRVQEISSLSRGLKLLSKELEVPMLVLSQLSRAPETRPGDHRPQLSDLRESGSIEQDA
                     DVVAFIFREEVYKQDREDLRGAAELLLSKQRNGPTGKIQLVFLHNLTKFENRLTDLGE
                     EPAPEE"
     gene            6022..7515
                     /locus_tag="VLE22_09235"
     CDS             6022..7515
                     /locus_tag="VLE22_09235"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HSB14630.1"
                     /translation="MLLELSNIQKKFGGVVALRNGDLSVDAGEVHLLLGENGAGKSTL
                     MKIVAGMLHRDSGVMRWQGREVDLRTPASAAAIGVAMVHQESLLAPHLSVAENIFLGR
                     EPRSLLGWVNRRRMMEQAARLIEEHRFPLQPEWRVERLSPAGRQLVEICRAICHGSSL
                     LIFDEPTSSLSDAETQEVFRIVRTLRERKMGVIYITHRLEELRAVGDRVTVLRDGETV
                     HAAPLQELTTDEIIHHMVGREVATIYERHSLPPGDELLRVEGLNRKQVLNGISFVLRA
                     GEIVGLAGLVGAGRTELCRALFGIDPVDAGTVRVLGRDVRIRSSQDAVKAGLALIPED
                     RQKNGLAVLLPLVHNVTHAGLDRVSPYGFLDLAAERRQASEYVQRLRIRSESIRQLAG
                     RLSGGNQQKVVIAKWLFRNARVFLFDEPTRGIDVGAKVEVFQLMDELARSGAAILMVS
                     SEMQELLQVADRILVMRHGRIAGELPGRTTQEQIMRYAALGAEDSVQ"
     gene            7512..8435
                     /locus_tag="VLE22_09240"
     CDS             7512..8435
                     /locus_tag="VLE22_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14631.1"
                     /translation="MIQRLLPSLSLIILFVGLSIASPHFLTQTNLSSVVRQTAVINVM
                     ALGMTLVIIAGGIDLSVGAILAMGGLLGTMAMEQGYPIPVGVFIGTVTGLAWGLVNGL
                     LTTKLAIAPFIVTLGTLGIVRGLTLIISNGLPVHRIPQSFSYLGEGNLLGVPFVLWIL
                     VVCAAVTHVILEHTRHGRYAFAIGSNPDAALYAGIPVAFHTTVVYAFCGMLTGLAGMI
                     EASRLMTGQPTAGQGYELQVIAAVVIGGGSLRGGEGSVIGTLIGAFIMGLLSNGSDLL
                     GISPYLQQAIIGAVIILAVTVDELRKRRLAS"
     gene            complement(8441..9079)
                     /locus_tag="VLE22_09245"
     CDS             complement(8441..9079)
                     /locus_tag="VLE22_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB14632.1"
                     /translation="MSKKIRILLADDHAVVRQGFGRILASEPDMEIVGEAATGHEAIA
                     LTEELQPDVVVMDVAMPELNGIEATRRLAASVPRARVLALSMHKDSVYVREILRAGAK
                     GYLLKDAVDRDLLAAVRAVARGEGYLSPAVSEAVLSDYRRHVTDPVDLLTSREREVLQ
                     MIAEGKTNKEIATLLNLSVYTVDAHRGRVMEKLNLHTTGELVRFAMRKGLID"
     gene            complement(9045..10400)
                     /locus_tag="VLE22_09250"
     CDS             complement(9045..10400)
                     /locus_tag="VLE22_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB14633.1"
                     /translation="MDTRSPNRQFHTSDQITHRNILRVLLAGFALVILLLVVAGIVAI
                     RNIRSIQANAATLVEEQKENTDLIEEVQREQAALSAVFYNLARDPESVDREKILTDLN
                     AVNENISELVSETADTPEEGLGKELGQAALAFSVEARRLLGLDNVPTRLSRDLLRRHQ
                     QVTAIVAKLVAGNQRKAQAAQQMIDHQSRRLVVQSFVLLGSCCLLALLFAMLTVKTTT
                     SLFRKMEWQASELSRVSWHMLGNQETTARRFSHELHDELGQSLTALKANLVSLRRPDT
                     PDRRLDDCLDLVDGAVRNVREISQLLHPTVLDDFGLESSLRWLAERFTERTRIEVDYV
                     SNFPGRVSPETETHLFRIAQEALTNVARHSGATKAKIELRANGDDVHLLIEDNGSGIQ
                     KEEANSSHGMGMVGMRARARNVGGELRIRTAPGQGVQIEISVPATARSHEQEDPHPAR
                     G"
     gene            10642..10812
                     /locus_tag="VLE22_09255"
     CDS             10642..10812
                     /locus_tag="VLE22_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14634.1"
                     /translation="MRKLIVALALAGLTLPAIVSAAPAPQEQKQEQKTEKKKKAPKKK
                     KGEKKEEAKPQA"
     gene            10845..12557
                     /locus_tag="VLE22_09260"
     CDS             10845..12557
                     /locus_tag="VLE22_09260"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HSB14635.1"
                     /translation="MGASRPPAASIPSALLTGEASDERPRVRRGVRVLIAALVTLGSG
                     VLNLWSVIGPSLPGRHALLRSIFPLEFLHLSRSVTLIIGFALIVSSINIYRRKRRAFQ
                     FAVLLVASSVVFHLTKGLDYEDAIFSGVFLALLWSARKNFTVRSSIPDWRAAAFRLAV
                     ASLVAVGYGIAGFWFLDPREFGINFTLADSIHRTVLFLSLVGDPQVVPHTRYAHWFVD
                     SLYLMTATAILYSGFSLFRPVLYRFRIHPHEEALAREIVARHGRCALDYFKTWPDKSF
                     FFSPDQQCFLAYRVGASFAVVLGDPVGPEDRIEGLVCDFLELCQENDWRIAFHQALPD
                     FLPVYLRLGFKKLKIGDDALVDLKAFSLEGKGMKATRNAVTKLEKSGVRLLQCEPPLT
                     DDVLAGLRDVSDEWLCIPGRRERQFTLGRFEPEYVRSTPVLIAASADGRYLGFVNIIK
                     SFRKGEATIDLMRRRTEAPNGLMDYLFVKLFLVCKERGYELFNLGMAPMAGFREKEEA
                     SPQERAVHQFFQHLNFLFSFKGLRAYKAKFANLWEPRYAVYRNAMDLPRLAIALGKVS
                     EIQP"
     gene            12554..13327
                     /locus_tag="VLE22_09265"
     CDS             12554..13327
                     /locus_tag="VLE22_09265"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB14636.1"
                     /translation="MKHPGCVAALVLVFCQFALAVQAGGKRAIEIRGRAQEVYYYPAA
                     SKPPRGKILFAPGDGGWRGFAVEIAQEASSWGYDVYGFDTKHYLESFTGKTMLSEADV
                     MKDMRSVASAIRSDAGERVIFIGWSEGAGLGVLAAAAAENKTTFQGLIAIGLGDSNVL
                     GWRWADNITYVTKQLPNEPAFSARKHLPNISPLPLFMIHSSKDEYTPARDARKLFEAA
                     RQPKRLSVIEAQNHRFAGNRDDFFRVLREAVEWARIAPR"
     gene            13303..14349
                     /locus_tag="VLE22_09270"
     CDS             13303..14349
                     /locus_tag="VLE22_09270"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSB14637.1"
                     /translation="MGPDRAPLGLAATGRAGNLERFLKPAAGYLLAAACLAWLFHDVH
                     PREVLRTASGMNWGWIAVAICFDIASYVSQGWRWKLLLAPLGRIGTLRATQAIYVGLF
                     ANEVLPMRAGEFARTWLVSRWLASDFSAVIPSIAVERLFDGVWLAICMGLTAIFVPLP
                     RNLAAAADVLGVAVLLATVLFLYLVYRKREQPPEAARAALQHWKPVRLLLSLIDALEL
                     GLRKIGVSRSFYLAFGGSLLLLAGQILAFWFVMLACGLNLSVWVGAVVLLIVHLGTLI
                     PNAPANLGTYQFFVVVGLGVFGVEKGAATGFSFIVFFLLTVPLWVIGLFALARTGMTL
                     TAIRREIGVQAPRT"
BASE COUNT         2624 a         4396 c         4584 g         2926 t
ORIGIN      
        1 acgggctggc tatcgaaact cgtaaaagac cgtttcgtct tcctggaaat cagatacagc
       61 tacccgaagt caagatcgcg ccggttcggt atcatcagca gtcaggaggc cagactcaca
      121 cgccatgaag gaacagaact acaccaacca ccggcagtac gttttcatgt accacgtcgt
      181 gttgttcggg ctgatcgtgc tgacgctcat cggctcgttc gtgaacctgt acaagtccat
      241 tggtgaccac cagcggctct atagcgcatc actgatcgta gtgatgaaca tctgtctcct
      301 cttgctgttc ttcctggcgc gggtcttcgc gctgaaggcg caggaccggg cgatccgggc
      361 ggaagagaac ctccggcact tcgtgctgac cggcaggccg ctggacagac ggttgacgat
      421 ccggcaggtt atcggattgc ggtttgcggc ggacgaagaa ttcgcggaac tggccaagcg
      481 cgcggtcaca gagagcctgt cggaagacgc gatcaagcgc gccgtgaaga gctggcgcgc
      541 cgacacgcac cgggtgtaaa cggtgtcagg aagttcgcac ggcggtccag cgcgccttgg
      601 ggaactcacc aaggagaagc tcgccctcca ttcgaccact ggagagcgtt ccctcgaaga
      661 catagaccaa ctgtgactcc tcgtagggaa gtacgctccg caggcggacc tggtcccctt
      721 ggacggtgcc tttgacgtca ccccgcaacc gcgacccggt gtgccttccg gtgacctcgt
      781 ctccccgagc ctcgaggtcg agcgtgtggc gtgcctcgcc acgaagatac cggatgctca
      841 cgtcccagcg gcccgagatg tcgctcgcgg ccggtttcgg taccgacgtt ttcggggaag
      901 gggctgagtg gagcaactga aacagccgat tcgcgacaat cctgtagtcg cctggtctca
      961 tcgcgacagg gcgcagaacg aaagaatgtc cctcaccctc ggcgtgcgta aggatcgccg
     1021 gctcgccttt gagcagcaga tcgtgcagct caccggcggt gaggccgatg cttgcgggat
     1081 cccacccaat ctcaagaata gggaaaggac cgccgcgcga cggaggcagc acgcgggtgg
     1141 agacgccgtc aacggccttg agccggctcg cgatgtgagc gtaccaagat tcccactctt
     1201 tgtactccgt ctcccggtta cggcggctca cccaggattc cactgccgtc accatgccga
     1261 cgatctcttc cttgctgacc ttcatcatgc ggccgaaagc gtggtgcggc gagctgttgg
     1321 cgtacgcggc gcgaacgaga tcctcgcggc cgaggagcag tccggcggtt tggggaccgc
     1381 gaaggatttt gccgcccgag taagcgacga ggtcggcgcc atcggcgatg tagggattcg
     1441 ggacgatgag gtagtccgcc gcggcgtcga cgagaacggg aactccggcc tggtgcgcga
     1501 tctccgacac ttccttgagc ggcaggctga cgttctcgaa gcggttgccg agcaccaggg
     1561 ccatcgcggt acggggaccg atggcggcac gcatctgttc gggtgaatcg acttcaacga
     1621 aacggacgcc gacggcgcgg acagcgtgaa agtactcgtt ccgcgacgat cgagggagaa
     1681 tgacttcgct cttgagaccg gccgtgtcgg gaagctgctt cattcgctcg gggtccgccc
     1741 cggcgacgca cgcggcggtg gcatggctca aggccgcggc tgcgccggac gagaccatcg
     1801 cccatggcac acggagcagt tgtgagatgc gcctgccggc ggcctccatc aattcgtcga
     1861 ggttgacgtg gtagtgggac gcctgttcga tcgcctcgat cacctcagga agcagacgcg
     1921 aaccgccgtt gatggtgtaa gtggcggtgc agttgataaa cggctggacg ccgaggcggg
     1981 tgtagacatc gggcgccggc tccttcccgg cagcgggcgc cgcgcccatc agtcccgctg
     2041 tggctgcgat gccgctcccg cggaacagct cacggcggga aaccggccgg cgatacgtgg
     2101 acggcttcgt tttcatagtc cggactcccc cggaatgtca tcgcaccgcg cggaggcggc
     2161 aaggagtccc tatgggaccc aggcgatgag ctcgatttcg acgcgggtgg ccgaatcggg
     2221 aaactcgaca ccgaaggccg agcgggccgg cgcgcctttc ttgaagtact cggcgtaggc
     2281 ctcgttgaag gcgggtttct cggttttcac gtcggccagg aacgcggtca ctttgatcac
     2341 gttttcgagc gacgagccca tggcttcgag attcttctta tgggcgtcga gggtacggcg
     2401 cgtctgttcc ttgacgtcgc cgccgaacgc cagcgggtct ttttgcgctt cagggcggac
     2461 gccggtcaga ccggaactga agtagaggtt gccgaccctg tacccgccgg gagccttgat
     2521 gcggccttcc ggggcggcgg cattcgccat cgccgccatg gcgccgccgg ttccgagacc
     2581 cgcggccgag cgcagaaatg tgcgtctgtt cttcgtcacg tggatagtgc ctcctagttg
     2641 acctcgctga acaacatacg ccgatagaga ttcctgcgca acctcgtggg aatcacgcca
     2701 tcggccggct gtaaccctgc cggcggtatc tctctagcag agcgctgagt tcctcgacct
     2761 tttcgggatg ttgttggtag aggttgcgat cctcgtgggg atcgccagcc aggttgtaca
     2821 actcgcccgc gggctcaccg ggtccgggct cgatccggtc gggtttggtg aagccgccgg
     2881 aaccgcggcc aagcaccagt ttccacgagc ctgagcggat gctgaaaagc ccctgcccgg
     2941 agtggctgac aatggcgtgg cgaatcggcc ggttcgcctt tccgaggagc gcagggagca
     3001 tgttgtagct gtcctcagcg tcgtcgcggc caagcgtacg gccggtaacg gcggcgaatg
     3061 tcgcgaacag gtcggtcagg caaagcgtct ggctgctgag ggagccggcc cggatgtggc
     3121 gcggccagag ggcgacgaac ggcacgcgat gacctgcttc ttggatgtct gacttctggc
     3181 cccgccacgg ccggttcgca cggtgcccgc cggaggcctg gatgtccggc tcggaccacg
     3241 gcgcgccgtt gtcgctagtg aagatgaaga gcgtgtcgcg tgctacgcct cggcgggaga
     3301 tcgtctcgat caactgcccg gtcgaggcgt ccacctgcgc gacgaagtcc ccgtactggc
     3361 ccgctttgga ttttccacgc catgccgcgg ccggcaccca cggcgtatgc ggcgctgtga
     3421 agggcacgta ggcgaagaac ggctgcaggg attcgcggac aaagcggacg gcgcgctcgg
     3481 tgatcttcgg caggacctcg tccatgcgga agccgggggc aatcgctccg ccgcgccaga
     3541 aggggccgcg aggcgaggcg ccgctgtcgg agatagtgga ggttggctgt tcgaccgccc
     3601 ggcggccgtc gatgtagaga tagggcggca tgtccagcga agcggggatg ccgaagaact
     3661 cgtcgaagcc gtaatcgagc gggctggggc ggagttcccg cgagtagtcc gccggctctc
     3721 gggttccgag gccgaggtgc cactttccga agcagccggt gcggtaaccg gagcgcttga
     3781 gcagagaggt gagtgtctcg cggccgtctt cgatgagcgc cggcgacgtc ccgtgaaaga
     3841 ctccgtgggt gaggcgcgtt ctccacgcgt agcgcccggt catcaggccg tagcgggtgg
     3901 gtgtgcagac ggcggagggc gagtgggcgt cggtgaagcg gacgccgtcg gaggccagcc
     3961 ggtcgatgtg aggcgtgcga atccgggatt cgtcgttgta gcaagcgata tcgccgtagc
     4021 cgagatcgtc gcagaggacg acgacgatat tcggtttctc cgccggctcg gcagcggcga
     4081 tggctgcggg gagcgatagg aaggtgcgcc gggtcagcat gatggcttca ggattaccgc
     4141 atcgtgaggt gggccgcaac gcacggaatg gcggaagcta gtgggagtcg aacccacccg
     4201 cggcgcggtc agcgtcgcac gctggttttg aagaccaggc ccagcaccgg ccaagattag
     4261 cttccacggc gattctaaca gaagcgtctc gtgagcagtg ccgcgggcgg ttcccagcag
     4321 gccgaggacg aaccgttacc cctctggctt tcctgctcat attcaggtaa aacagaagca
     4381 gatggatttg ttttcggaaa atctgtggaa aaggctgcca cgcaaaaagg gagttgttgg
     4441 tctgcaatga ctttttcgtt cggacggccg gacgaaacac agggcggatt tccacgacct
     4501 aaccgccaga aacggggcgg gtaatttttc gctataccgg aggcgggctg ccggttaatc
     4561 tggagcggtt gtcgaatggc caccaccgat ctcgcactgc aaaagggact tcctgcaaat
     4621 actgacgcgg agcgtttcgt cctaggttcg atcctgctcg atgacgctgc ctacgtccag
     4681 gttgcatcag cgctcgacgc gagtgacttc agcctcgaga agcaccggcg cattttcctg
     4741 cggatggccg agcttcacga gcacggtgag aagatcgatc gggttacggt tgccaacgag
     4801 ctgatcaagc acaaccagct cgaatcctgc gacggtttgg gatacctcgt ctctctcgat
     4861 gacgggctgc cgcagatcta caacctggac agctacgtcc ggatcgtaaa ggacaagtcg
     4921 ctgctgaggc ggctgatctt cacgtctcag aaactgatcg accgctgcat gatgggcgac
     4981 gaggaaccca cggaaatcct cgccagcgcc gaggaaaccc ttctcaaact cggcgagacg
     5041 accacgaagg tgggcctggc cacgcccgcc gaggtcatcg agggttacga aggcgggctg
     5101 aacacgttcc tcgatccggg taagcgcatc cgcggattga gcaccggatt caagaaattc
     5161 gacgagatga ctggcggatt gcaccccggt gagctaatca ttctcgccgg acggccctcg
     5221 atgggaaaaa ccgcactggc attgaacatc gcccagcacg cagcggtcaa gctcggcaaa
     5281 accgttgcgg tgttctcgct tgagatgtcc aatgagtcgc tgctcacgag acttctgtgc
     5341 gccggagccc gggtcgacag ccatcgattc cggctcggat accttgatgg cgatgagcgc
     5401 cggcgcctcc aggccgccgc aatggaactg gtggaggcgc cgcttttcat cgatgacact
     5461 tcgggaagca acctcattga catgcatgcc aagctcaggc gcctgaaggc cgaacggcag
     5521 cttgatctag tggtggtgga ttatctccag ctaatgagcg gccgtggacg gttcgacagc
     5581 cgcgtgcagg aaatcagctc gctgtcgcgc ggcctgaagc tgctctccaa ggaattggag
     5641 gtgccgatgc tcgtgctttc ccagttgagc cgcgcaccgg agacacgacc tggcgatcac
     5701 cgcccccagc tcagcgacct ccgcgaatcc ggatccatcg agcaggatgc cgatgtcgtc
     5761 gcgttcatct tccgcgaaga agtctataag caggatcggg aagatctgcg gggtgcagcg
     5821 gaactgttgc tctccaagca gagaaacggc ccgacgggaa agatccagct tgtctttctg
     5881 cacaacctga ccaagttcga gaaccgcctc accgacttgg gcgaggaacc ggcgccggaa
     5941 gagtaacgtc ccgcgatcgt acgtgtccgg cgccctcgac ggatcacgcg acaggccact
     6001 cattgtaaac tgtcgtttcc tgtgcttctg gagctaagca acatccaaaa gaagttcggc
     6061 ggcgtcgttg cgctccgtaa cggcgacctt tccgtcgacg ccggcgaagt ccacctccta
     6121 cttggcgaga atggcgccgg caagtccacg ctaatgaaga tcgtggccgg catgctccac
     6181 cgtgacagtg gcgtcatgcg ctggcagggc agggaagtgg acctgaggac gcccgcgagt
     6241 gccgctgcca tcggtgtcgc catggtgcac caggaatcgt tgctcgctcc ccatctgagc
     6301 gtggcggaga acatcttcct tggccgtgag ccgcgttctc tcctaggttg ggtgaaccgc
     6361 cgtaggatga tggaacaagc cgcgcggctg atcgaggaac accgatttcc actccagccg
     6421 gaatggcgcg tggagcggct gagccccgcc ggccgccaac tcgtggaaat ctgccgcgcg
     6481 atctgtcacg gatcgagcct gctgatcttc gacgagccca cttcgtccct gtcggacgcc
     6541 gagacgcagg aggtcttccg catcgtgcgc acgttacgcg agcggaagat gggcgtcatc
     6601 tacatcaccc accggctgga ggagcttcga gcggtcggtg atcgtgtcac cgtcctgaga
     6661 gacggcgaaa ccgtgcacgc cgcgccactt caggagctca cgacggacga aatcatccac
     6721 cacatggtgg ggcgcgaagt ggcgacgatc tatgagcgcc actcgctgcc tccgggcgac
     6781 gagttgctgc gtgtagaagg cttgaaccgt aagcaagttc tgaacgggat cagcttcgtc
     6841 ttgcgcgccg gagaaatcgt aggcctggcc ggcttggtgg gcgcgggccg cacggagctc
     6901 tgccgtgccc tgttcggcat cgatcccgtt gacgccggaa ctgtgcgggt tctcggccgg
     6961 gatgtacgga ttcgctcttc gcaagacgcc gtgaaggcgg gtctcgccct gatccccgag
     7021 gaccgccaga agaatggctt ggcggtcctg ctcccgcttg tccacaatgt cactcacgcc
     7081 ggactcgatc gcgtcagccc gtacggattc ctggaccttg cggccgagag gaggcaggcg
     7141 tcggaatatg tgcagcggct tcgcatccgc tcagaatcga tacggcagct tgcgggccgt
     7201 ctgagcggcg ggaaccagca gaaagtcgtc atcgccaagt ggttgtttcg caatgcccga
     7261 gttttcctgt ttgacgaacc gacgcgcgga attgacgtcg gcgccaaggt cgaggtgttt
     7321 cagttgatgg atgaacttgc ccgttcggga gccgccattc tgatggtcag ctccgagatg
     7381 caggaactgc ttcaggtggc ggaccggatc ctggtcatgc ggcacggcag gatcgcgggc
     7441 gagttgccgg gccggaccac ccaggagcag atcatgcgct acgccgcgct cggcgccgag
     7501 gatagtgtcc aatgatccag cggcttttgc cgtctctcag cctgatcatc ctgttcgtgg
     7561 gcctgtcgat cgcatcgccc cattttttga cccagacgaa cctgtcgagc gttgtccggc
     7621 agacggcggt gatcaatgtc atggccctcg gcatgaccct ggtcatcatc gcgggcggca
     7681 tcgatctctc ggtcggggcc atcctcgcca tgggaggcct gctcgggacg atggcgatgg
     7741 agcagggtta ccccattccg gtaggtgtgt tcatcgggac agtgacgggg ttggcgtggg
     7801 ggctggtcaa tggattgctc acgacgaagc tcgccatcgc tccgttcatc gtcacgttgg
     7861 gtactctggg catcgttcgc ggcttgacgc tgatcatctc gaatggcctg ccagtacacc
     7921 gcattccgca gtcattctct tacctcgggg agggcaatct gctcggcgtg ccgttcgtgc
     7981 tgtggattct cgtggtgtgt gctgccgtca cccatgtaat cctggagcac acgcgtcacg
     8041 gcaggtatgc cttcgcgatc gggagtaacc cggacgcagc tctctacgcc ggcattccgg
     8101 tcgcgttcca cacgactgtt gtctatgcct tctgcgggat gcttacgggc ctcgcgggca
     8161 tgatcgaagc atcgcgcttg atgaccgggc aaccgacagc ggggcaaggt tatgagttac
     8221 aagtgatcgc cgccgtggtc atcggcggtg gcagcctgcg gggaggcgag ggcagcgtca
     8281 tcggtacgct gatcggtgcg tttatcatgg gcctgctctc caacggcagc gacctgctgg
     8341 gtatctcccc ttacctccaa caggccatta tcggcgccgt catcatcctg gcggtaaccg
     8401 tggatgagct gcgcaagcgc aggttggcct cctgagcccg tcagtcgatc aggcccttcc
     8461 gcattgcaaa gcggacgagt tctcccgtcg tgtgaaggtt cagtttttcc atgacgcggc
     8521 cgcggtgtgc atcgacggtg tatacgctca gattgagcag tgtcgcgatc tccttgttcg
     8581 tttttccctc ggcaatcatc tggagcacct cgcgctcacg gctcgtgagc aggtccaccg
     8641 ggtcagtcac atgcctccgg taatcgctta gaacggcttc ggaaacagcc gggctcaggt
     8701 agccttcgcc cctcgccacg gcgcgtacgg cggccaggag atcgcggtcg accgcgtcct
     8761 ttagcaggta tcccttggca ccggctcgca ggatctcacg cacgtacacc gagtccttgt
     8821 gcatgctcaa cgcaagtacg cgagctcggg gcacgctggc ggccagcctg cgcgtcgcct
     8881 cgatcccatt caactccggc atggcgacgt ccatcacaac cacatccggc tggagttcct
     8941 cggtcagtgc aatggcctca tgaccggtgg cggcctcacc gacgatctcc atatccggct
     9001 cggatgcgag aatccttccg aaaccctgcc ggacgactgc gtgatcatcc gcgagcagga
     9061 tgcggatctt cttgctcatg actcctcgca gttgcaggca cgctgatctc gatttgcaca
     9121 ccctggcccg gcgccgtgcg gatccggagt tccccgccca cattgcgggc gcgggcgcgc
     9181 atgccgacca ttcccattcc gtgtgaagag tttgcctctt ctttctgaat gccgctcccg
     9241 ttatcttcga tcagcaggtg gacgtcgtcg ccattggccc tgagttcgat tttcgctttg
     9301 gtcgctccgg agtggcgggc gacgttagtg agggcctcct gggcgatgcg gaacaagtga
     9361 gtttcggttt ccggcgacac gcggccgggg aaattcgata cgtagtccac ctcaatgcgc
     9421 gtgcgttccg tgaacctctc cgcgagccag cgcaggctcg attcgagtcc gaagtcgtcc
     9481 agcactgtcg ggtgtaataa ctgtgagatc tcccgaacgt tgcggactgc gccgtccacc
     9541 agatccaggc aatcatcgag ccgtctgtca ggcgtatcag gacggcgcaa agaaaccaga
     9601 ttcgctttca acgcggtcag tgattgaccc agctcgtcgt gcagttcgtg agagaagcgc
     9661 cgggcggtgg tttcctggtt ccctagcatg tgccaggaca cccggctcaa ttcgcttgcc
     9721 tgccactcca tcttgcgaaa gagactcgtc gtggtcttga ccgtcagcat ggcgaatagc
     9781 agagccagca gacagcagga ccccagcagg acgaacgact gcaccaccag ccttctcgat
     9841 tggtgatcga tcatctgctg cgccgcctgt gctttacgct gattcccggc gacgagtttg
     9901 gctacgatag ccgtcacctg ttgatgtcgg cggaggagat ctcgcgacaa acgtgtgggc
     9961 acgttgtcca acccgagcaa ccgccgggct tcgacggaaa acgcgagcgc agcctgcccc
    10021 agttccttgc cgagaccctc ctcgggggtg tccgccgtct cgctgaccag ttcactgatg
    10081 ttctcattta ccgcgttgag atcggtcagg attttctccc ggtcgaccga ctcggggtca
    10141 cgggcaagat tatagaaaac cgcgctgaga gcggcctgct cccgctgaac ctcctcgatc
    10201 agatcggtat tttccttttg ttcttccaca agggtagctg cgttcgcctg aatcgagcgg
    10261 atgtttctga ttgcgacgat cccggccacc acgagcagaa ggatgacaag cgcgaaaccg
    10321 gccaggagca cacggaggat attccggtgc gtaatctggt cgctggtgtg gaactgccgg
    10381 ttcgggctcc tcgtgtccac ctcctttcca gtgtaaggca cccgctacgg gagtcccata
    10441 gcccacagcg gctacgcggc actcctaagt cacggtaggc tcctttctta gcattgcggg
    10501 aacggtggcc gcactgaacg caatcccctg atttcggcag tcttcgtagc cctcggaggc
    10561 tattctctct ccggtcatcg gcggcgaaca tggaagcgtg cccgccggac ttacttggca
    10621 agcacaaagg agaacagaag aatgaggaag ctaatcgtcg ccctggcgct tgcagggctg
    10681 acattgccgg ccatcgtgtc cgcggctccg gctccgcagg agcagaagca ggagcagaag
    10741 accgaaaaga agaagaaggc cccgaaaaag aagaagggcg aaaagaaaga ggaagcaaag
    10801 ccccaggcct gacctgtcta acggtgtaca ctggcgggag gctaatgggt gcgtcacgtc
    10861 ctcccgccgc ctcgataccg tccgccctgc tcacgggaga ggccagcgat gagcggcccc
    10921 gcgtaaggcg cggagttcga gttctgattg ccgcccttgt cacgcttggc agcggcgtct
    10981 tgaacctctg gtcggtgatc gggccgagtc tgcccgggcg ccacgctctc ctacgatcga
    11041 ttttcccgct cgaattcctg cacctctccc gttccgtgac actcattatc ggtttcgcgc
    11101 tgatcgtttc ctcgattaac atctaccggc gcaagcggcg ggctttccag ttcgcggtct
    11161 tgctggtggc atcttccgtg gtctttcacc tcacaaaggg gctcgattac gaagacgcca
    11221 tcttttcagg agtttttctt gcgcttctct ggtccgcccg aaagaacttc acagtaagaa
    11281 gcagtatccc agactggcgc gcggctgctt tccggctcgc cgtggcgtcg ctggttgcgg
    11341 ttggatacgg aattgccggg ttctggttcc tagacccgag ggagttcggc atcaacttca
    11401 cgctcgctga ctcgatccac cggacagtcc tcttcctctc tctggtgggc gaccctcaag
    11461 tggttccaca tacacggtac gcgcactggt tcgtcgattc gctctacctg atgacggcga
    11521 cggcaatcct ctattctggt ttctcgttgt tccgccccgt cctctaccgg tttcggattc
    11581 acccgcatga agaggcgctc gcccgcgaga tcgtcgcccg tcatgggcgc tgcgcgttgg
    11641 actacttcaa gacctggccg gacaagtcgt tcttcttttc gccggatcag caatgcttcc
    11701 tcgcctatcg tgtcggcgcg agtttcgccg tcgtgttggg ggatccggtg ggtcccgaag
    11761 accgcatcga aggactagtc tgcgactttc tggagttgtg ccaagagaac gactggagga
    11821 ttgcctttca ccaagcgctc ccagatttcc tgcctgttta cctccgcctc gggttcaaga
    11881 aactcaagat cggcgatgat gctcttgtcg atctcaaggc tttctccctg gagggcaagg
    11941 ggatgaaagc tacccggaac gctgtcacga agctggagaa atccggggtc cgcctgctcc
    12001 agtgtgaacc gccccttact gacgacgtgc tggcaggact ccgggacgtt tccgacgagt
    12061 ggctctgcat ccccggacgg cgggagcgcc agttcactct cggcaggttc gagcctgaat
    12121 acgtgcgctc gacgcccgtg ctcattgccg cctccgctga cggccgttat ctcggctttg
    12181 tgaacatcat caaatccttc aggaagggag aggcaacaat cgacctaatg cgtcgccgca
    12241 ccgaggctcc taacggcctc atggactacc tgttcgtaaa gctctttctc gtgtgcaagg
    12301 agagaggcta cgagttgttc aacctcggca tggcgcctat ggcggggttt cgggagaaag
    12361 aggaggcctc accccaggaa cgcgccgtac accagttctt tcagcacctg aactttctgt
    12421 tcagcttcaa gggtttgcgt gcttacaaag ccaagttcgc aaatctctgg gagccgcgct
    12481 acgccgtgta ccggaacgcg atggatctgc cgcgcctcgc gatcgcgctg ggcaaagtct
    12541 cggagataca accatgaagc accccggttg cgtcgctgct ctggtgctcg tgttctgcca
    12601 gttcgctcta gctgtccagg ctggcggcaa gcgtgcgatc gagatccgtg gcagggcgca
    12661 ggaagtgtat tattacccgg ctgcgtccaa accgcctcgc ggaaagatac ttttcgcgcc
    12721 gggcgatgga gggtggcggg gttttgcggt cgaaatcgca caagaagcgt cttcgtgggg
    12781 ctacgatgtc tacgggttcg acacgaaaca ctacctggag agtttcaccg gcaaaaccat
    12841 gctgtctgag gccgacgtga tgaaggacat gcggtcggtc gcctccgcca ttcgcagcga
    12901 tgccggcgag agggtcatct ttatcggctg gtcggagggc gccgggctcg gggtgctggc
    12961 ggccgcggct gcggagaaca agaccacctt ccagggcctg atcgcgatcg gcctgggtga
    13021 ctcaaatgtg cttggctggc gctgggctga caacattact tacgtcacca agcagttgcc
    13081 gaatgagccg gcgttctccg ccagaaaaca tctgccaaat atctctccac taccgctgtt
    13141 catgattcat tcgagcaaag acgaatacac tccggcgagg gacgcccgga agttgttcga
    13201 ggcggcgcgg cagccaaagc gactctctgt gatcgaggcc cagaaccacc gcttcgccgg
    13261 caaccgcgat gacttcttcc gcgttctccg ggaggctgtg gaatgggccc ggatcgcgcc
    13321 ccgctaggtc tggccgccac agggcgtgcc gggaacctcg aacgttttct caaacctgcg
    13381 gccggctatc tgctcgcagc cgcctgcctc gcatggctct tccacgatgt tcaccctcgc
    13441 gaggtgctcc gaaccgcttc ggggatgaac tgggggtgga tagcggtggc gatctgcttc
    13501 gatatcgcga gctacgtcag ccagggatgg cgctggaagt tacttctggc tccgctgggg
    13561 cgaattggga cacttcgggc aacccaggcc atctacgtgg gactcttcgc caacgaggtg
    13621 cttcccatgc gggcgggcga gttcgcacgc acatggctcg tgtcgcgatg gctcgcctct
    13681 gacttctccg cagtgatccc ttcaatcgcc gttgagcggc tctttgacgg cgtctggctg
    13741 gcgatctgca tgggcctgac ggccattttc gttccgctgc cccggaatct cgccgcggcc
    13801 gccgatgttc tcggcgtcgc tgtcctgctg gcgacagtcc tgttcctgta ccttgtctac
    13861 cgaaaacgag agcaaccgcc cgaagcggcc cgagccgcct tgcagcactg gaaaccggtg
    13921 cgtctattgc tctcactgat cgatgcgctc gaattgggac tccggaagat cggtgtgtcc
    13981 cgttcgttct acctggcgtt cggcgggtca ctgctgctgc tcgccggcca aatccttgcc
    14041 ttttggtttg tcatgctggc ttgcggacta aatctctcgg tgtgggtcgg tgcggtggtt
    14101 ttgttgattg tccacctcgg cacgttgatc cccaacgccc cggcgaacct tggcacctac
    14161 cagttctttg ttgttgtcgg tctcggagtg ttcggcgtag agaagggagc cgctaccgga
    14221 ttctcgttca tcgtcttttt tcttttgaca gtgccgcttt gggtgatcgg cctgttcgcg
    14281 ctcgcccgta ccggcatgac tctcacggcc attcggcgcg agatcggtgt tcaggcgccg
    14341 cgaacctgac gccgatgttc atcatggatg cccgccgtct cgcaggttgc gcggcgtcgt
    14401 catcgacggg tccggaattg gtcggcaatc gccgcgaact agggttacgg gtattcgcgc
    14461 agccctgtgt tatgataggg cttctgctcg caacagtcgg gaatacaacg actcgaacgc
    14521 gctcacttta
//
LOCUS       DATIFQ010000200         4660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1215555, whole genome shotgun sequence.
ACCESSION   DATIFQ010000200 DATIFQ010000000
VERSION     DATIFQ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215555"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(658..963)
                     /locus_tag="VLE22_09275"
     CDS             complement(658..963)
                     /locus_tag="VLE22_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14638.1"
                     /translation="MKLLGIALGGLASLYITASAGLYIAMRQPPERFGAIMSKVPMAA
                     MMILPFRPLWMSARAGQLAVGQAAPDFALQTVDRSRIVRLSQEWRDRPVVLIFGSYT"
     gene            complement(1036..4311)
                     /locus_tag="VLE22_09280"
     CDS             complement(1036..4311)
                     /locus_tag="VLE22_09280"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB14639.1"
                     /translation="MILYQSLRIVVVVCFFFATATGQEPTGGIGGSVTDPSGATVPRA
                     RVTVTHKQTGQSQLQTTVDAGLFTFPVLPAGDYELVVEANGFAPLRLSQVKVEIDRTI
                     RLQLELVITGAERIEVSAVSETVDRDSNTLGQTVTERHVTDLPLNGRNFTQLGLLQAG
                     VAPLTSGLISAGGSIKATQAFAVNGQRPESNNFLLDGVRNVNRVDGGFAIRTPIDALQ
                     EFRILTHTAPPEYGSASAASVSVITRSGGNSAHGTVYYFGRNDVVDARNFFSAGVEPL
                     KQHQYGATLGGPIRRNRLFFFGYYEGFRNRQGVTKTATVPTPAERAGDFSALQDPSTN
                     NLLINFLSGQPFPGNRIPDEMFNGVSQRVLDFYPLGNVSPSLFRATEVLRNDVDQGGI
                     RIDAIARETDQVAIRYATSSGSNFNPLSIRGADVPGFATGDDIRAHSATISHTHVFSP
                     RTLHNVRGAFSRYQFLFDKRFNHTTPRSLGFQYDNTFEPALGPPYFIVNGYASVGNPI
                     TGPRNSAQNDYEIDDTLSLVRGAHTIKVGGGFRRTQLNISQGIAANGFFVFAPFPTNS
                     SFANFLIGFPVVFFQGGGDFQRGLRSFEVSAYAQDQWRVTRKLTLNYGLRYEVNSPFA
                     EIRDRLNEFAPGQQSQVFPDAPRGILFPGDPGVAKRILDIYYKGLMPRIGIAYDPTGS
                     GRMSVRAAYGVFFDSFSNGTGMPMQAAISAIPFLQAVQLGPPALNYTNPWGATGVPFR
                     PGFFPKPVTMLTEDSAGRPPYAQNWNFSIQQSLPSAMLFEVRYVGTKGTRVPRFIEAN
                     PSVYTPGATSQSVDAKRIYAGCPAAGGPCDFASVGLVNYSTNSTYHALQATLSRRFNR
                     GLTFSTSYWFSKTLDYVSSMNVAGSAPRLVSGENDIAQNPFDLRAEHGPSLFDAKHRW
                     TASVTWELPFGKTLQGVSRAVLGGWQLNGIATVTSRTPFTVYDSRNVAQQGSHPEITG
                     FAGSRPDLIADPNRGARTVEQWISRSAFRRLDAVAEAGRFGNAGRNIARGPGQGALDV
                     SAFKNWSLSERVRAQLRVEAFNVTNHPNFGVPVNDLVSPNFGRIVEAAPPRVFQAALK
                     ILF"
BASE COUNT          983 a         1335 c         1481 g          861 t
ORIGIN      
        1 gaggcacacg aaggacaata ttcttaaacc cactcgctct cgcactcccc ataagccatc
       61 tctagccccg aaggaccccc tccgtcaagc gagagcggca ccagccgcac gcctgtacgt
      121 accggaggca ttgtgcctcc ctctgcgtac cgcgtcaagg accagatcgc gagtgggccc
      181 atccggagga gggtcaccaa gacaagccgg ggctcagtcg ggggaggtgc aagctcgtta
      241 gaggctgcca ggagccggtt aattcgcgtt cgtcgtaatt cgtcgaagag actgctcgag
      301 atctcgcgct gagaaaccgt agggccctgg cgcgctcctg aacacaatgc gcccgtttct
      361 atcgatcaag tacatacggt caggccaggc tgtatatgcg cgctccgtcc ggttatcgat
      421 gccatccaga accgcaggga cgcgaagacc gagcttccgg acgcacgtca aggccgtgga
      481 agtccgctcg ccatctgatc gcggagaggc aaccaaaaca ccatccctga cgttcggtgg
      541 caactgccag agatcggtgg gatgggcctc ctgaatgtac acaatgtaga acgctatacg
      601 atcctggtac aactgatata gtcgattgag ctcgggaacc tcccggcgaa atggcggtca
      661 agtataacta ccgaagatca gcacaaccgg ccggtcccgc cactcctgtg acagcctgac
      721 aatgcggctg cggtccacag tttgcagcgc gaaatcgggc gctgcctgtc ccacagcaag
      781 ctgtcccgcc cgagcagaca tccacaaagg cctgaaggga agaatcatca tcgcggccat
      841 tggaactttc gacatgatcg cgccgaagcg ttccggcggc tggcgcatcg cgatgtagag
      901 gccagcggat gctgtgatgt atagcgaagc cagtccgccc agtgcgatcc caagcagttt
      961 catcaggcgc actcaacacc tccgggcaac cgtagcagaa ctcgatacaa aggacattct
     1021 ccttttgaac cggcgctaga aaaggatctt gagggcggcc tggaagaccc gcggcggcgc
     1081 ggcttcgacg atgcgtccga aattcggaga caccagatcg ttcacgggaa caccgaaatt
     1141 ggggtgattg gtcacgttga atgcctcgac ccggagctgc gcgcgcacgc gttcggacaa
     1201 actccaattc ttgaaggcag agacgtccaa ggcgccctgc ccggggccgc gagcgatgtt
     1261 gcggccggca ttgccgaacc gtccggcttc ggcgacggcg tccaggcggc ggaaggcgga
     1321 tcgcgagatc cactgctcca ccgtgcgggc accacgattc gggtcggcaa tcaggtcggg
     1381 ccggctgccc gcaaagcccg tgatctcggg gtgcgatccc tgctgcgcaa cgttgcgcga
     1441 gtcatacact gtgaacggcg ttctcgacgt cactgtcgcg attccgttca gttgccaccc
     1501 tcccagcact gctcgggata cgccctgtag tgttttgccg aagggcagct cccatgtgac
     1561 actcgccgtc cagcgatgtt tggcgtcgaa gagtgaaggt ccatgttccg cgcgcagatc
     1621 gaacggattc tgcgcgatgt cgttctcgcc cgacacaagc cgaggcgccg atccggcgac
     1681 gttcatcgat gaaacgtagt cgagcgtctt ggagaaccag tacgacgtgc tgaacgtcag
     1741 cccgcggtta aagcgccggg agagcgttgc ttgcagcgcg tggtacgtgg agtttgtgga
     1801 gtaattcacc aggccgaccg acgcgaaatc gcagggtccg cccgccgctg ggcagccggc
     1861 atagatcctt ttggcatcga cactctgcga cgtcgcgccg ggcgtgtaca cagatggatt
     1921 ggcctcgatg aagcgcggaa cgcgcgtccc ttttgtgccc acatagcgga cttcgaagag
     1981 catggcggac ggcaacgact gttggatcga gaagttccaa ttctgcgcgt atggcggacg
     2041 gcctgccgag tcctctgtca gcatggtgac gggcttggga aagaaaccgg gccgaaacgg
     2101 cacgccagtt gccccccacg gattcgtgta gttcagcgcc ggcgggccga gttgcactgc
     2161 ctgcaggaat gggattgcgc tgatggcggc ctgcatcggc atgccggtgc cattggagaa
     2221 cgagtcgaag aagaccccgt atgccgcacg cacgctcatg cggccgcttc cggtaggatc
     2281 gtaagcgatt ccgatacgcg gcatcagtcc cttgtagtag atgtcgagga tgcgcttggc
     2341 aaccccagga tcgccgggga acaaaatgcc ccgcggcgca tcgggaaaaa cctgcgactg
     2401 ctggcccggc gcgaactcat tcaaccggtc gcggatctca gcgaacggcg aattgacctc
     2461 gtagcgtaat ccgtagttga gcgtaagctt gcgtgtgacg cgccactggt cctgagcgta
     2521 ggcactcact tcaaagctgc gcaagccgcg ttgaaagtcg ccgccaccct ggaagaacac
     2581 aacgggaaaa ccgatcagaa agttcgcaaa cgagctattg gtgggaaacg gcgcgaacac
     2641 gaagaagccg ttagccgcga ttccctggct gatgttgagc tgggttctcc ggaagccgcc
     2701 accgaccttg atcgtgtgcg cgcctcgcac cagcgacagc gtgtcatcaa tctcgtagtc
     2761 gttttgagcg ctgttgcggg ggccggtaat cgggtttccg acgctggcat agccgttcac
     2821 aatgaaatac ggcggcccca atgcgggctc gaaggtgttg tcgtactgaa acccgagaga
     2881 ccggggggtt gtgtgattga agcgcttgtc gaaaaggaac tggtagcggg agaacgcgcc
     2941 cctcacgttg tgcagtgtcc gcgggctgaa cacgtgcgta tgcgaaatgg tcgcggaatg
     3001 cgcgcggatg tcgtcgccgg tggcaaaccc cggcacgtcg gcgccgcgga tggaaagcgg
     3061 gttgaagttc gatccggatg aagtcgcgta gcggatggca acctggtccg tttctcgggc
     3121 gatcgcgtca atccgaatgc ctccctggtc gacgtcattc cgcaggacct cggtagcccg
     3181 gaaaagcgac ggcgagacat tccccaaggg atagaagtcg agcacccgct gtgagacacc
     3241 gttgaacatt tcgtccggaa tacggttgcc cgggaacggc tgtccgctca ggaaatttat
     3301 caggaggttg ttggtgctcg ggtcctggag cgcggagaag tcgccggcac gctcagccgg
     3361 cgttgggact gtggccgtct ttgtgacacc ctggcgattt cggaatcctt cgtagtagcc
     3421 gaagaagaag agccggttgc gacggatagg accgcccaga gtcgcgccgt actgatgttg
     3481 cttgagcggc tcgacccccg cggagaaaaa gtttcgcgcg tccaccacgt cgttgcgtcc
     3541 gaagtagtag accgttccat gagcggagtt accgccggat cgcgtgatca cggaaacgga
     3601 cgcagcgctt gccgatccgt actcgggagg cgcggtgtgc gtgagaatgc ggaactcctg
     3661 aagcgcatcg atcggcgtac ggatcgcaaa gccgccatcc acccgattga cgttccgcac
     3721 accgtccagc agaaagttgt tcgattcggg gcgttgccca ttgacggcga aagcctgggt
     3781 ggctttgatg gagccgccgg cactgataag cccgctcgtc agcggcgcca ccccggcttg
     3841 caacagtccg agctgcgtga aattacgtcc gttgagcggg agatctgtga catggcgctc
     3901 agtgacggtt tggcccagcg tgtttgaatc gcggtctacg gtctctgata cggcggacac
     3961 ctcgatccgt tcggcgccgg tgatgacgag ttcgagctgc agcctgatgg tacggtcgat
     4021 ttccaccttc acctgcgaca accggagcgg tgcaaacccg ttggcctcga cgacaagctc
     4081 atagtcgccg gctggaagaa ctgggaacgt gaaaagcccg gcgtcgacgg tggtttgaag
     4141 ctggctctgc ccagtttgct tgtgcgtaac ggttacacgg gcacgcggca cagtggcgcc
     4201 tgacggatcg gttaccgagc cgccgatgcc gccggtcggc tcctgtcccg tcgcggttgc
     4261 gaaaaaaaag cacacaacga caacgattct caacgattga taaaggatca ttcccagaag
     4321 tcccgcacag aataccagtg gtccagagga cgaggcaata atctctttct actaacggcg
     4381 gtagcagtcg gggatatgct ggtggtagca ggatgcgaag agggaaattc tgacttcgac
     4441 agtccagatg aggtccgtat cgggacgctg aatctataat cgagggtagt gacgaaacaa
     4501 gaacggaact ctcggcaaag acaaatcttg ggtagtgacg ggcgtgcggc cggttgagga
     4561 gggtggtttc gggtgggagc ccgggacagg atgcgaaagg aaaaggcgat gcgatggatt
     4621 gcagccacag tgcttggatt ggcggtgtta gggacatcgg
//
LOCUS       DATIFQ010000201         4065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1597702, whole genome shotgun sequence.
ACCESSION   DATIFQ010000201 DATIFQ010000000
VERSION     DATIFQ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597702"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VLE22_09285"
     CDS             complement(<1..200)
                     /locus_tag="VLE22_09285"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HSB14640.1"
                     /translation="MSNHFTGLSLGPPLGDPRLDLCDLYAFQSPADGNRTVIILNANP
                     NADALHPDAIYRLNIDNDGDYLT"
     gene            complement(637..1554)
                     /locus_tag="VLE22_09290"
     CDS             complement(637..1554)
                     /locus_tag="VLE22_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB14641.1"
                     /translation="MSLANAAQPVRAGLDELVPSRYALRVGEIEVLVISDGVLMLPGA
                     MLGYNADPAVRGAWLEDKFLPPDVFEWALNAVVVRSGGRTILIDAGIGADPGLNLPRA
                     GRLVQRLEAAGIDLPSVTDVVLTHMHMDHIGGLLVDGVRDRLRPDLRINAAAAEVKFW
                     ASPDFSRVNMPPGFPDALRRTAKRFLNEYDSQMRPFDNDYEVAPGVLVRRTGGHTPGH
                     SVVRLASGGDRLTFAGDAVFQVGFEHPDWFNGFEHNPEEAARVRVGLLRELAANRESL
                     VATHLSFPSVCHVAVDGDVFRCVPGPWEY"
     gene            complement(1884..2831)
                     /locus_tag="VLE22_09295"
     CDS             complement(1884..2831)
                     /locus_tag="VLE22_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14642.1"
                     /translation="MKDPAINQITATLSENRVTAAAVSPDGSRLAYSEFGGSLIIQDL
                     KSGGRRLLRAPERTLIETISWIGSSGKILISGETEAEVPTLWTEDLSDSQLYTLRQDA
                     RRGIASPDGQLIAFTSVDETEIWTMKADGGDVRKLVAGVVVDTFPFMAFSSDNRRVSY
                     QRRHCRQYVYRELDPVRGRGKELARTAWTPSLLGDWTLSAAGAEAAMPNHDPRQARIL
                     LIALDASPGHAPATRELQVPELGGLLKLVAADANDNGWFASATSLQGVKLLHIDRNGR
                     FRVLRECPITTWEVPSPDGRRLAFVDQAAFSNFWSVGLF"
     gene            complement(3306..>4065)
                     /locus_tag="VLE22_09300"
     CDS             complement(3306..>4065)
                     /locus_tag="VLE22_09300"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HSB14643.1"
                     /translation="AKEAESRSAEAVGKKIISDGEVSFGPKPNIMTAGSIKFFAGSRS
                     DAFFFDFDGIKGLFDTSGKRNFTEPHLGGKSPWTGVDSNTEANVFSTVIELPTSELNP
                     KPEIHIWGRCSIRRDGKLLHVDRAGHPSVSSFFNTDDTKEEYNASEPVNDRNRWLDQF
                     VHLMGHTGNYTREEAIAAIDNERTLPDVLSFDPSKPAKYPNGRVFTDDVINYRLAFLT
                     KNQCPPTGLKPHTDILTEFPYIGTPHSQKLAAGW"
BASE COUNT          810 a         1282 c         1194 g          779 t
ORIGIN      
        1 gtcaggtaat cgccatcgtt atcgatgttg aggcgataga tcgcgtccgg atggagtgcg
       61 tccgcattgg gattcgcatt caggatgatc acggttctat tcccgtcagc gggcgactgg
      121 aatgcgtaca gatcgcacag gtcgaggcgt ggatcgccca gcggcgggcc gagactgagc
      181 ccagtgaaat ggtttgacat tgcccctctc ctttccactg atattcgcac attcaggaaa
      241 tcgaggccac cgtcgatcaa gaccgtcgtg aacagccgtc cgtgaacgct gcacgattgc
      301 cgtcattgaa ggaaccggtg ggtctgtgca cttaaccacg gataaccctc ttatcgcgct
      361 ttgccgggaa gcaagcgaag gaacttcgcg aaccgcaacc tgcggcaatc gcaaacggag
      421 aacttcgtag gagacgaaga agaacgtctt gtctttaatc agccgtcccc cgagcgtgcc
      481 gcacgccagg atgccgtgga actgctcgac gacttccggc tcaccgtccg ccatccccga
      541 tgccctccgc cgacgaacga cacggctcac ctgccggggc cgcctttgtg accccggtct
      601 ggtgcggcgc ctattgggcc ctaaccaaca aagcgatcag tactcccagg gccccggcac
      661 gcaacgaaag acgtcgccat cgaccgccac atggcagacg gacgggaacg acagatgagt
      721 ggcgaccagc gactcgcggt tcgccgccag ctcccgcaga agaccgaccc ggacgcgagc
      781 cgcctcctcg gggttgtgtt cgaagccgtt gaaccagtcg gggtgctcga acccaacctg
      841 gaacacggcg tcgcccgcga acgtcaaccg gtcgccgccg gacgccaggc ggactacgct
      901 gtgcccaggg gtgtggccgc cggtgcggcg gacgagcacc cccggcgcca cctcgtagtc
      961 gttatcaaaa ggccgcatct ggctgtcgta ctcgttcaag aaccgcttgg ccgtccgccg
     1021 aagtgcgtcc gggaagcccg gcggcatgtt gacgcgggag aaatcgggcg acgcccagaa
     1081 cttgacctcg gcggccgccg cgttgatccg caggtccgga cgtagccggt ccctcacccc
     1141 gtcgacgagc agcccgccaa tgtggtccat gtgcatgtgg gtaagcacca cgtcggtcac
     1201 ggaggggaga tcgatgccgg cggcctccag tcgctggacc aaccgtccgg cccgtggcaa
     1261 gtttaagccc gggtccgcgc ctatcccagc gtcgatgagt atggttcggc cgccgctgcg
     1321 caccacaacc gcattgagcg cccactcgaa cacgtccggc ggcaggaact tgtcctccag
     1381 ccaggccccc cggacggccg ggtcggcgtt gtagcccaac atcgcgcccg gcagcattag
     1441 caccccatcg ctgatcacca ggacctcaat ctcgccgacc cgcagagcgt agcgcgacgg
     1501 aaccaactcg tcgagccccg ctctaacggg ctgtgcggcg ttcgccaagc tcatttttgt
     1561 atcctcctgc tgacggcctt acccacggcc gcatcttgcg ccgccatccg ggtgacgcgc
     1621 ccgaaaaaca tggcaacgct gggagcattg tcgctttcgc tggattgact taacggtgat
     1681 ggtagtgcgg tcaagcattg atgtcgccac cgaaaggacc cagcgtggca gacgcgacgc
     1741 ccgtggccga cctcctgccc caacccgggt acgccgcacg attgatctga ggctatatta
     1801 gcacgtcgcg tgcgcagtgg gaagcggaaa acgaaagaga atgaaagaga acccctcacc
     1861 aatttgcggg aaattttgct gtgtcagaat aacccaacgg accagaagtt gctgaaggca
     1921 gcctggtcga caaatgcgag ccgccggccg tctggagagg gcacctccca agtggtgatc
     1981 ggacattccc gcagaacccg gaatcgcccg ttgcgatcga tgtgcagcaa cttcactccc
     2041 tgtaaggaag tggcggaagc gaaccatccg ttatcgttgg cgtcggcggc tacaagctta
     2101 agcaaaccac cgagttccgg aacctgcagc tctctggtcg ctggcgcatg acctggcgag
     2161 gcatcgagag cgatcagaag gattcgcgct tgccgtggat catgattggg catggccgcc
     2221 tccgcaccag cggcggagag cgtccaatca cccagaagcg acggagtcca ggcggtgcgc
     2281 gcaagctcct tgccgcgccc ccgcaccgga tccagttccc tgtaaacata ctgccgacag
     2341 tggcgccgct ggtatgagac gcgccgatta tcggaagaga aggccatgaa aggaaaggta
     2401 tcgacgacga cgccggccac aagcttacgc acatcccctc catcggcctt catggtccag
     2461 atttcggttt cgtcgacgct ggtaaacgcg ataagttgac cgtctggcga cgcgattccg
     2521 cggcgggcgt cctgccggag ggtgtacagc tgggaatcgg agagatcctc cgtccacaga
     2581 gttggaacct ctgcctccgt ttcgccgctg atgagaatct tacccgacga cccaatccac
     2641 gagatcgttt cgatcagagt tctctccggc gcccgaagaa gccgcctgcc accgctcttc
     2701 aggtcttgga tgatgagact tcccccgaat tccgagtatg ccagtctgct cccatcaggg
     2761 gacacggcag cggccgttac ccggttctca gacagagtgg ctgtgatctg attgatcgca
     2821 ggatccttca agcccgcgtt tctgaccgca taccaccagg ccacgacacc cgcaagaagg
     2881 atgaaaacga acgcgcccca aacccagatc gcggacgaga gccgtctgtt gctatgtttg
     2941 aacctcgccg attccgcagc ggtccattcc cagtcgtccg ccacgtcctg caggtctggc
     3001 tgtacttgcg gggcggacga tggcggtccg tctggatact tcgactacca ccgaggcccc
     3061 ctttagcgca tctgcgagtc cctcgccggt aagactgttg acgccggaac tgggcgccgc
     3121 ggctacggct tcgtgcccat gctcaccaag ctttttgaca agcttcgatc caatgagtcc
     3181 ggtaccgcca attacgacga ttttcatgat tctcctttcg tattgaaccg gcgcgattct
     3241 cgattgccga ttgttcgcta cgaccgaacg ccaaggaagc taggagcaat gctccggaat
     3301 cggactcacc atccagctgc taacttttgc gaatgcggcg tgccgatgta cgggaactcc
     3361 gtcagaatgt cggtgtgggg cttgaggcct gtgggcggac actgattctt cgtcaggaag
     3421 gcgaggcggt agttgatcac gtcgtcagtg aagacgcgcc cgttgggata cttcgcgggc
     3481 ttcgacggat cgaaacttag cacatcggga agtgtgcgct cgttgtcgat cgcggcaatc
     3541 gcttcctcgc gcgtgtaatt gcccgtatgc cccatcagat gcacgaactg gtcgagccat
     3601 cggttgcggt cgttgaccgg ctcgctcgcg ttgtattctt ccttggtgtc atcggtgttg
     3661 aaaaagctgc taacgctcgg atggcccgct cgatcgacgt gtagcaactt gccgtctcgt
     3721 cttatactgc atcgccccca aatgtggatc tcgggcttgg gattgagttc gctcgtcggc
     3781 agctcgatca cagtcgaaaa cacgttcgct tccgtattcg aatccacgcc ggtccatgga
     3841 gacttgccgc cgagatgagg ctcggtgaag ttccgtttgc cgctcgtatc gaacaggccc
     3901 ttgattccgt cgaagtcgaa aaaaaaagcg tcactgcggc tgccggcaaa aaatttgatg
     3961 ctgcccgcgg tcatgatgtt gggcttgggt ccgaacgaga cttcaccgtc cgagatgatc
     4021 ttcttcccaa cggcttccgc tgagcgcgac tccgcctcct tcgcc
//
LOCUS       DATIFQ010000202         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1597920, whole genome shotgun sequence.
ACCESSION   DATIFQ010000202 DATIFQ010000000
VERSION     DATIFQ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597920"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..2330)
                     /locus_tag="VLE22_09305"
     CDS             complement(66..2330)
                     /locus_tag="VLE22_09305"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14644.1"
                     /translation="MAVDEQLLQAVMPLCQDVAPDILQDFVGRMDQEYFRRFHPETIA
                     RHVHLAALLTPDQPCKLAITEHDGQPLEMTIVAYDYFAEFATICGLLSAFGLNIEEGQ
                     IYTYAEAAATASSKPAYGGGPRQRPKTRPGLSRKKIVDVFRVKPVQGASFGSEDQQPL
                     EKELSEVIRLLDTGQVEEARQTVNRHLVEHLGQRRGSFSGLLHPVQITFDNSQSPTDT
                     IMDIRSDDTPAFLYAFANALAMRNIYIAKAQIELDGGKLHDRFYVRNRYGQKLTDPAD
                     QQQLRLTAVLIKQFTHALTWAPDPTKALEAFDQFLDLIVQETKGKAQQEFLAFLSDKK
                     TFPLLARLLGASDFLWEDFLRRQHDNLLPLLQDYRDAPLIKPQAALRKELDRLVGRAK
                     TEQARKDALNHFKDRELFRIDMKHIVEPSTSLADFSLALTELAEVIVQRSFNDCQAKL
                     HRQYGAPTLPNKKPCSFAILGIGKFGGRELGYASDIELLFVYGGMGRTSGKQKIENSE
                     YFERLVQELLQWIEAKQEGIFHLDVRLRPHGGKGSLANPLDEITNYYSDKGLAAPFER
                     QAFIKLRHVAGDAVLGKQVEAHRDRYVYSGEAWDLTTALDLRRQQLKQLVERGTVNVK
                     LSPGGLVDIEYAIQYLQLMHGHKHPVLRTPNSMQALAGLIECGFVTRQDGENLRKAYL
                     FIRMLIDGLRMVRGHAKDLVLPPPDSDEFIFLARRVGYTTDDWQAGARHLKTDIEQHM
                     RNTKAFFENTFGAV"
BASE COUNT          474 a          714 c          798 g          587 t
ORIGIN      
        1 aattcccgcg tgtcgcgttc ggcgagcacg gggaccgacc aggccagcta tctggaatca
       61 tcttcttata ctgccccgaa tgtgttttcg aaaaatgcct ttgtgtttct catatgctgc
      121 tcgatatctg tctttaaatg cctcgcccct gcctgccagt cgtccgtcgt atacccgacc
      181 cgccgcgcga gaaaaatgaa ctcgtctgaa tcaggcggcg ggagcaccag gtccttagca
      241 tggcctcgaa ccatcctcag accgtcgatc agcatgcgaa tgaacaagta agccttccgc
      301 agattctcac cgtcctgccg cgtgacaaat ccacattcga tcaacccagc cagcgcttgc
      361 atcgagttcg gcgtacgcaa aacgggatgc ttatggccat gcatgagttg gagatactgg
      421 atggcatact cgatatcgac gagaccgccg gggctcaact tcacgttgac cgttccgcgt
      481 tcgaccagtt gcttgagctg ttgccgccgc agatcgagag cggtagtcag atcccaggct
      541 tcgccgctgt agacgtaacg gtcccgatgg gcctccacct gttttcccag caccgcatct
      601 cctgccacgt gccgcagttt gatgaaggcc tgccgctcga acggcgcagc caatcccttg
      661 tcgctgtagt aattggtaat ttcgtccagg ggattggcca gcgatccctt gcccccatgg
      721 gggcgcaggc ggacgtcgag gtgaaagatg ccttcctgtt tggcctcaat ccattggagg
      781 agttcctgca cgaggcgttc gaagtattcg ctgttctcga tcttctgctt gccgctcgtc
      841 cgtcccatgc cgccgtacac gaacaggagc tcgatgtcgg aggcatagcc cagttcgcgc
      901 cctccgaact tgccgatgcc caggattgca aaggagcagg gtttcttatt cgggagtgtg
      961 ggcgcgccgt attgcctgtg cagtttggct tggcagtcgt tgaagctccg ctggacgatg
     1021 acttcggcga gttccgtcag ggctagtgaa aagtctgcga ggctcgtgct tggctcgacg
     1081 atgtgtttca tgtcgatgcg gaagagctcg cgatctttga agtggttcaa cgcatctttc
     1141 cgcgcctgct cggtcttggc gcggcccaca aggcggtcga gttccttccg tagtgcggct
     1201 tggggtttga tgagcggcgc atcgcggtag tcttgcagca gcggcaggag gttgtcgtgt
     1261 tgccggcgca ggaagtcctc ccagagaaaa tcgctcgcgc cgagcaggcg ggcgaggagc
     1321 gggaaggttt tcttgtcgct gaggaacgcc aggaattctt gctgggcctt gcctttcgtc
     1381 tcctgcacga tgagatcgag gaattggtcg aaagcttcca gggccttcgt cgggtccggg
     1441 gcccaggtaa gggcgtgcgt gaactgtttg ataagcacag ccgtaagacg cagctgctgc
     1501 tggtcggcgg ggtccgttag tttctgcccg tagcggttgc ggacgtaaaa ccggtcgtgg
     1561 agcttgccgc cgtcgagttc gatctgtgcc ttggcgatgt agatgttgcg catggccagg
     1621 gcattggcga aggcgtagag aaaggccggc gtatcgtccg agcggatgtc catgatcgtg
     1681 tccgtcggcg actggctgtt gtcgaaggtg atctgcacgg gatggagcag gccgctgaac
     1741 gatccgcggc gctgtccgag gtgttcgacg aggtgccggt tcacggtctg gcgggcttcc
     1801 tcaacttgtc ctgtgtccag caaccggatg acctcgctca gttctttctc caggggctgt
     1861 tgatcttccg atccgaacga ggcgccctgg accggcttca cgcgaaagac gtcgacgatt
     1921 ttttttcggc tcaggccggg gcgagttttg gggcgctggc gagggccgcc tccataggcg
     1981 ggcttggacg atgcggtcgc agctgcttcg gcataggtat agatttggcc ttcttcgatg
     2041 ttcaaaccga aggcggagag caggccgcag atcgtggcga actccgcaaa gtagtcgtac
     2101 gcgacgatcg tcatctccag cggctgacca tcatgctcgg tgatggcgag tttgcagggc
     2161 tgatccggtg tcaagagggc ggccaggtgg acgtgccgcg caatggtctc cggatggaat
     2221 cgccggaagt attcctggtc catgcgcccg acgaagtcct gcaggatatc gggagcgaca
     2281 tcctggcaga gaggcatcac tgcctgcagg agctgttcat cgacggccat agaaatcgaa
     2341 tcctttggct ctcagcactc agctatcagc attcagcttt cggaaagaag accgttctgt
     2401 tgttatgcta acggctgaca gctgatggct gaaagctcgc tgccgcagta taccggaacg
     2461 ccttcattgt gcgtagggga gcctgtcagt ataatgccgc gccatgacta gcaggctgtt
     2521 gaaaaagtcc accagctgcg ttctcgcttc gctcaaaggc tcaacgtaca gta
//
LOCUS       DATIFQ010000203         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_832762, whole genome shotgun sequence.
ACCESSION   DATIFQ010000203 DATIFQ010000000
VERSION     DATIFQ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832762"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VLE22_09310"
     CDS             <1..865
                     /locus_tag="VLE22_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14645.1"
                     /translation="LQKPPSIKFKDLEAAVSSKITYNLLPMKVRADYVRVTNSSILTN
                     VTALFDKKDLQFQEKDGVQKAIVNIYGRITSMSRRVINVFEDVVTVDVPTELLQEAIK
                     GQSIYGKSIPLPPGMYRLNIVAKDVVGGNMNNYEIALKVPHYDEETLSSSSLILADLI
                     EKVPTRSIGTGQFVIGTSKVRPRVDDSFRQAEKMGVYIQFYNFKPDEKTQKPNGVIEY
                     EVVKDGTNEKILEFSEEVSAIDGASAAQVTVEKLLPLQTLVPGKYTLKMKVVDRNRDA
                     TLTTAANFRIS"
     gene            902..2701
                     /locus_tag="VLE22_09315"
     CDS             902..2701
                     /locus_tag="VLE22_09315"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB14646.1"
                     /translation="MNVRTFGIVAGVVAVWLFVTTAAAAPPDKLAGSIGGSVTSTIGI
                     PQMGATVLLYNHAERLLQRVLTDETGSFLFDALAPDVYSIRVSLTSFVPALKRNIIVQ
                     PGMRSLLNVSLSGVLSSIELIYSSTGNRAVMSDDWKWVLRASSSTRPVLRIAPRIDIS
                     DPAERGRSQSVFGETRGMVKVSAGDSSTVSAYGNEPDLGTAFALATSVYGANQLQVSG
                     NIGYAAVSGMPTAGFRTRYSRNLPGGNDPEVRVTMRQLFLPTRTGTAVVTGQTDGVPA
                     LRTMSFGFQDLAQLTDNIRAEYGFSLESVSFLERLNYLSPFARITYGQDETGAIEFGY
                     SSGLPPAEFFAMKGEAGNDLQRDLAALSLFPRVSLRDGRVRVQRTENFEIGYRKVTGS
                     RTFSMAAYSESLNNAAITMVAPAGAIPSSDLLPDLLSNSSVFNLGRYAGFGYMGSFTQ
                     ALAESLSLTGAFGSGTALTPSAQQLLTEKPDEVRGIMRHGRRYWLAAKLSAKAPRAGT
                     LLTTSYRWADGRSLNAGHYYLTQNLRPEIGWSVYLRQPIPAFSGLPGRFEATADLRNL
                     LAEGYVPLLTPQGRRVYLLHTPRSVRGGLSFIF"
     gene            complement(3089..>3286)
                     /locus_tag="VLE22_09320"
     CDS             complement(3089..>3286)
                     /locus_tag="VLE22_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14647.1"
                     /translation="RIPMHGVALQCHFRPLIQEIAELADEKLYQAENLGSGTFRITKP
                     KRKSRKSRQSKLKNPQRGARK"
BASE COUNT          663 a         1005 c          936 g          682 t
ORIGIN      
        1 gctccagaag cctccttcca tcaagttcaa ggacctcgag gccgccgtca gttccaagat
       61 tacgtacaac cttcttccga tgaaggttcg agccgactac gtgagggtca cgaactcctc
      121 catcctgacc aacgtcaccg ccctgtttga caagaaagac ctccagttcc aggagaagga
      181 cggggtccag aaagcgatcg tcaacatcta cgggcgaatc acgtcgatgt cgcgccgggt
      241 gatcaacgtt ttcgaggacg tggtcacggt tgatgtgccc acggaactgc ttcaggaagc
      301 aatcaaggga cagtcgatct acgggaaatc gatacctttg ccccctggca tgtaccgcct
      361 aaacatcgtc gccaaagacg tggtaggcgg caacatgaac aactacgaaa tcgcgctgaa
      421 agtgccgcac tacgacgagg aaacgctgtc ttcgagcagc ctgattctgg ccgacttgat
      481 cgagaaggtg ccgacccgga gcatcggcac gggccagttc gtcattggca cttccaaggt
      541 ccgcccgcgg gtggacgatt ctttccgcca ggccgagaag atgggtgttt acatccagtt
      601 ttacaacttc aagcccgatg aaaagaccca gaagccgaac ggggtcatcg agtacgaagt
      661 ggtaaaggac ggaaccaacg agaaaattct cgagttcagc gaggaagtct ccgcaattga
      721 cggagcgtcc gccgctcaag tgacggtcga gaaactgctg cccttacaga cgctcgtccc
      781 cggcaagtac accttgaaga tgaaggtcgt cgaccggaat cgggacgcta cgttgacgac
      841 ggcggcaaac tttaggatat cctaggagtg gtttgaatca gcgggagggc gccgctctcg
      901 gatgaacgtc aggacgttcg gaatcgtggc cggggttgtc gccgtgtggt tgtttgtcac
      961 aacggcagcg gctgctcccc cggacaaact cgccggtagc atcggcggtt ccgtcaccag
     1021 cacgattggc atcccgcaga tgggcgccac cgtgctcctt tacaaccacg ctgaacgatt
     1081 gctccagcgt gtccttaccg acgagaccgg aagtttcctg tttgacgctc tcgccccgga
     1141 cgtgtattcg atccgggtca gcctgaccag tttcgtgcca gcgctcaaac ggaacatcat
     1201 cgtccaacct gggatgcgca gcctgttgaa tgtcagcctt tcgggcgtgc tcagttcgat
     1261 tgagttgatc tattcatcaa ccggaaacag agcagtgatg agcgacgact ggaagtgggt
     1321 tctccgggct tccagcagca cccggccggt cttgcggatc gcacccagga ttgatatttc
     1381 cgaccccgcc gaacgcggcc gctcgcagtc cgtctttggc gagacccgtg gcatggtgaa
     1441 ggtatcggcg ggggacagca gcactgtctc tgcgtacggg aatgagcctg atcttggaac
     1501 cgcgttcgct ctggcaacct cggtatacgg cgccaaccag ttgcaggtga gtggcaacat
     1561 tggctacgcc gccgtctctg gcatgccgac ggctggattc cgcacgcgct acagccggaa
     1621 ccttcccggc gggaacgatc cggaagtccg ggtgacgatg cgccagttgt tcctgccgac
     1681 gcgcacgggg accgcggttg tgacggggca gacagatgga gttccggccc tccgtacgat
     1741 gtcatttggc ttccaggatc ttgcccagct caccgacaat atccgggccg aatacggttt
     1801 ttcgctcgag tcggtctcgt tccttgagcg gctcaactac ctcagcccct tcgctcgtat
     1861 cacctatggg caggatgaga ccggcgcgat cgagttcggt tacagttccg gcctgccgcc
     1921 ggcggaattc ttcgccatga aaggggaggc cggcaacgac cttcagaggg atctcgcggc
     1981 gctttcccta ttccccaggg tctctctccg tgatggcagg gtacgcgtgc aacgcacaga
     2041 gaacttcgag atcggctacc gcaaggtcac tggatcgaga accttcagta tggccgccta
     2101 ttccgagtcc ctcaacaacg cggccattac aatggtggcc ccggccgggg cgataccttc
     2161 cagcgacctg ctgcctgatc tcctttctaa cagctcggtc ttcaacttgg gcaggtacgc
     2221 gggctttggc tacatgggat ccttcaccca ggctctggcg gagagtctca gccttacggg
     2281 tgctttcggc agcggtacgg cgctgacgcc gtctgcacaa caattactca cggaaaaacc
     2341 ggacgaagta cgtggaatca tgcgtcacgg ccggcgctac tggcttgcgg cgaaactctc
     2401 cgcgaaggcg cctcgggccg gcacactgct gacgacaagt taccgctggg cggacggtcg
     2461 ctcgctgaat gccggccact actacctcac ccagaacctg cggcccgaga ttggctggag
     2521 cgtctacctt cggcagccta tccccgcttt ctcaggactt ccgggccgct tcgaagcgac
     2581 cgctgacctc cgcaatctcc ttgccgaagg ttacgttccc ctgcttaccc cacaggggcg
     2641 tcgcgtctac ctgctccaca ctccgcgcag cgtccgcggc ggcctcagct tcatcttcta
     2701 gcgccattcc aacttgaaag tcgccgccga ggtcgttagc tgcgaccccg cgatcagccg
     2761 tttgagagtc agcaagttgg aacggcacta gtggcagcat tcacagaatg cgctcggcta
     2821 tttttccttt gttttcaata cacgtccgcg gaattggctc tgcgatccca gaaggcgtgc
     2881 catatcttgg agccgaagag ggagttcggt ccagccgtca tcaccctccg cagtggtccc
     2941 gatttccttc ccagacacac cttcccgctc ccaccgtgga gcgatcctac agagcaccgg
     3001 attccctctg gagttctcaa aaggagaaac gaccgcgcct gatgtacagg cacactcgac
     3061 agtaacagat tgctcgcgcg acgtgctctt acttcctggc gccgcgctgc ggatttttga
     3121 gtttcgattg ccgcgatttc cgcgactttc gcttcggctt ggtgatgcgg aaggtgccgc
     3181 ttcccagatt ctcagcctga tacagctttt cgtcagccaa ttctgcaatc tcctggatta
     3241 agggtcggaa atgacattgt agcgcaaccc cgtgcatggg aatgcg
//
LOCUS       DATIFQ010000204        17224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1791405, whole genome shotgun sequence.
ACCESSION   DATIFQ010000204 DATIFQ010000000
VERSION     DATIFQ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17224
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791405"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VLE22_09325"
     CDS             complement(<1..551)
                     /locus_tag="VLE22_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14648.1"
                     /translation="MIDGLACRRKQFESILFGPMRKGEFDLNYHELRLELELFRRSGK
                     SFQDFFEQIMQRADSSFIMVKPMGKEGDWKADGYSLESKTIYQCYAPEKMTGVEAAKK
                     IEEDFEGARTRWQERMRRWVFVWSSERALPPQAVAALAGLKATHPELEITDLSRAGLW
                     VIVKGLPLADREVLLGIVPDLNDA"
     gene            complement(833..1720)
                     /locus_tag="VLE22_09330"
     CDS             complement(833..1720)
                     /locus_tag="VLE22_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HSB14649.1"
                     /translation="MTRFESSSGLEGALRDRVLARLGFTAPPSADLDGLHAIYRAWCV
                     GVPFDNVRKMIALRADRSPILPGGHAGPFFEDWLATGTGGTCWPTSNALFELLRSLGF
                     DACRAAGAMRDLGIVNHATVKVRIGNRDWLADSSLLSNAPLPLDESVFIHDDPVIPAE
                     VEFEDGTHVVWSHTPPNSSYLPCRLIADRESHAGYLDYYEQSRNRSPFNSRLYARRNR
                     PGETVVLIGNTRFAKTAEGVDRRDLSPDELRDVLHHDIGLSEGIIDEWGRSGSLAASF
                     EPPSGPKPPAVTRKPPSQR"
     gene            1793..3151
                     /locus_tag="VLE22_09335"
     CDS             1793..3151
                     /locus_tag="VLE22_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB14650.1"
                     /translation="MKVLLIQPQSSNPLMDRVYLFEPLALEYVGAGLKQAGHEVRLVD
                     ARLEPDAERVFTEFRPEAVGITGFTTHLNIVKHLAARLKNRDPKVLIVVGGHHATVRP
                     DDFAGTAVDVVVIGEGVFAMVELLQAVERGEEPLTVRGLALQGPTGLIYTEPRAPSPL
                     DVLPMPDRNLTAAHRGSYFSEWFRPLASVRTSLGCTARCTFCALWSITGGKYLRRDPE
                     NVVRELQTIAEANVFFCDDESMVDVKRMSRLADLIAESGIRKRYFLYARVDTIVKHPE
                     LFGKWKRIGLSQVFVGMEDFSDARLVAMQKGISTEQQAMAVKILSDLGVMMYASFMVD
                     PAYEVEDFRLLREYIRRLRLTYATFAVLTPLPGTELHGSVKNRLLSQKPELYDMLHAL
                     VPTRLPLPEFYEQMARLYAGAIPLFRSLPVLLRYGLHGILVRLRVYETFLARVRASHL
                     DY"
     gene            3176..3784
                     /locus_tag="VLE22_09340"
     CDS             3176..3784
                     /locus_tag="VLE22_09340"
                     /inference="COORDINATES: protein motif:HMM:NF025338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSB14651.1"
                     /translation="MNTRDKIIETAIRLFNERGTRAVTTNHISAGAGISPGNLYYHFR
                     NKEEIIRAIFRQMDVYGREEHRRIHERTTGTIETIEESFVMIQKFNWRFRFFKRELTS
                     LVNADPALKEDFVRTHRETLVLVRSSIDRSIEHGLLLSLPEEDRQFLAEAIWLVTLFW
                     PNYLEVSGEEVNEASLQRGSDLLRRILAPYVVQRPKRRSRPG"
     gene            complement(3781..4266)
                     /locus_tag="VLE22_09345"
     CDS             complement(3781..4266)
                     /locus_tag="VLE22_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14652.1"
                     /translation="MASRNTTRRAVLDQWLRENRPARIGDAEQRQIHDLLAPTSDSYL
                     RKLLRSCGVPLEPLVEGVRQDSYDDLERTLLALGQIYERASERDDRELATRARREVLM
                     AKEHARLAARSRRASAEVQSLKQEMISWMTVWLEDPSLFPTWLRLRKGVLFDSSEPRA
                     D"
     gene            complement(4190..5230)
                     /gene="mltG"
                     /locus_tag="VLE22_09350"
     CDS             complement(4190..5230)
                     /gene="mltG"
                     /locus_tag="VLE22_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HSB14653.1"
                     /translation="MKWLVVTLLLAVCLLGLAAFSLSQPYSGFQNQVFLDIPRGTGTV
                     QLSGLLAGAGVVRYRWQFLAARTLRPRVTLQAGEYRFREPATALDVLDRIARGDVFFY
                     ELRVPEGNNIFDIADSLDQLGLLPRDGFLEAARDPSLIRDLAPEAPTLEGYLFPDTYR
                     LTRQTTATEICRLMTTRFREAWRSLGVDAPVHRAVTLASLVEKETGLSEERAAIASVF
                     HNRLRIGMPLDCDPTTIYAAMLEERYRGTIFRSDLLSRNRYNTYQHSGLPPGPIANPG
                     LESLKAALEPADTPYLYFVARPDGSGRHQFSSELDAHNRAVRNYRRGIQEHNQAGGAR
                     PVAEGKPARANR"
     gene            complement(5227..5688)
                     /gene="ruvX"
                     /locus_tag="VLE22_09355"
     CDS             complement(5227..5688)
                     /gene="ruvX"
                     /locus_tag="VLE22_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HSB14654.1"
                     /translation="MNENNLQYPGRILALDLGRRRIGLAMSDELRITAQGLDTLARTS
                     LRDDLAALTQLASDRGVSLIVIGNPIRLNGVEGAQSVWVREFASKLEACTGLPVRLWD
                     ERFTTVEAERVLRESGISREKRGRAVDRLAAVILLESYLGSIDTAAENAEA"
     gene            5759..6934
                     /locus_tag="VLE22_09360"
     CDS             5759..6934
                     /locus_tag="VLE22_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSB14655.1"
                     /translation="MAVLASSIRTLRPKNAASAVLAAAAIIGLLYYGRVFFITLIIAI
                     TIAFILEPFVSFAMRLRLPRAVASFVVCALALLVLYLAGLGFYTQFADLYRELPKYSQ
                     RIGELTEVVTQKVEEMERSTYRLLLPRRMQRQEQQPAQGEPPRAAAKTQPRRRSAEPP
                     MPIGPPPVQEVRILSDERTPVVNYLYTHLGLVYEVLLMASFVPFLVYFMLSWRDHIYR
                     SFLQLFEGQDRLVAGNSLEGIASMVRGFVVGNFVLGVLLASASSLLFWAFNLPYPLLI
                     GPLSGFLSLVPYVGLPLAMVPPLFGALTVLKTAPAYLLLATLVSVLHLLALNLFYPKV
                     VGSRVHLNPLVVTVALMVWGVLWGAAGLVLAIPMTAGIKAVCDNVTSLQPYGRLMGD"
     gene            6958..7458
                     /locus_tag="VLE22_09365"
     CDS             6958..7458
                     /locus_tag="VLE22_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14656.1"
                     /translation="MLKKQFLTYVGIGLVLVAIGVAVIFHIQRGAHIELRGSVLKVRT
                     LPIDNASAIAVIDFRFVNPADYPFVVRRVNVSMEDPAGRVIEGSVISESDAVRLFQYY
                     PVLGQKFNESLTIRTKVAPRQAMDRMIGARFELSDKELEGRKRLTIRIEDVDGAISDV
                     VEEARP"
     gene            complement(7493..9169)
                     /locus_tag="VLE22_09370"
     CDS             complement(7493..9169)
                     /locus_tag="VLE22_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14657.1"
                     /translation="MQDISVLGLVASRLASVKSPPVQEHQLFEDVFRPPRAHWPSFAA
                     SALFHVFVIVVLLPLTSMLSDSEEEVWRRHARTFRPLEIQIPDRLYLSSTQVAIAPRP
                     VVEKPKPEEKRGLKAPSQQMARAASPAPAPLPKAEPDRVLPKQFRVPNVTHRPEADQT
                     LIQPHLPPELQLTAKVQLPNILLSAPTLPRPAAKKFAEPGPSAPSVVIPRVDAPPALA
                     GSGSTTSDLYVPSMVAGPQNALLHLPRPTIQGRPFKGPVSTASGQGASVSPISGEPIN
                     VLALSSNPGQLKDQISIPLGNQIGRIPPPPPGADDALGRPGIPGSKAGGTGTLASLGS
                     GLGGSGTGLGDGPGGIGTASAGSGTGTGTGTGTGTGTGTGTGAGAGGVAGTGGLAPGL
                     KYASAIRIEHPSNAQFDVVVMQSSADEGFPESAGVLSGQPVYTVYLQVGAPRAWLLQY
                     CIPKDTGPVTKVVGGAVYIGKATPVKAPFPLVTVLPPATMMPRSSYIMVHGFVDKAGK
                     FKDMNVLRAPADQIRELLIPQLTEWVFRPATRDGIPVLVEVLLAIPPHEG"
     gene            complement(9222..9959)
                     /locus_tag="VLE22_09375"
     CDS             complement(9222..9959)
                     /locus_tag="VLE22_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB14658.1"
                     /translation="MTRLYLLTDEPVLAAGLRSILSTAGGFTLDAVCETTTQLVAEIE
                     AKRPDLLLLDLRSDLTFGSLVEFQRRFPAIKVVLWVRAISTELAYQAIEHGIRGILRK
                     TLDPETLFKCLQMVADGGLWFEETLKASFDGVRTISLTRRESQLVSLLSQGLKNKEIA
                     ATLFISEGTVKVYLSRLFHKLGVKDRFELALYGLKNMPAGEALVEMPQPRTVQPPRKD
                     VNQEQWLRSLVVEKPQLKVRGPGTVFD"
     gene            10333..10713
                     /locus_tag="VLE22_09380"
     CDS             10333..10713
                     /locus_tag="VLE22_09380"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB14659.1"
                     /translation="MERRRSSRLDISLQCRVSSPRIGSRVLMGITENMSRGDVLVLLD
                     GEQALPELPCVGDPLIVEIELPENHAFGRKCMQCQTTVVRVSQSEEGVPRVALRIHKM
                     RFQSCGKDSVQLIGQRTGVRQLLM"
     gene            10750..11247
                     /locus_tag="VLE22_09385"
     CDS             10750..11247
                     /locus_tag="VLE22_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HSB14660.1"
                     /translation="MKDKTDKRVRKTAKKESGHAVIEASLLAPWIFFLFIGVFDFGFY
                     SYAAIATQNAARIAAMQTSDNTKTAADDVLACQYAVQELQMLPGIPKVTTLANTPACP
                     TATGGITNTFPLSVDAQSVTGPDGAPATRVAVTYRTVQLVPIPGLMTGRLQLTRVVEM
                     RLKES"
     gene            11251..11760
                     /locus_tag="VLE22_09390"
     CDS             11251..11760
                     /locus_tag="VLE22_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HSB14661.1"
                     /translation="MPDCKNKRRGDVMLEFTLVGIPLIFILISVVEMARGMWTYHTLA
                     HAVKEGTRYAIVHGYNCSLAPNNCQVTVAQIAQRVREAGVGLMPNELRIELRSEGDTV
                     LCSTLDNCLTNNNFWPVYPNSQIGQRVTINGTYPFRSAIAMFWPGAGGGMNFGTFLLP
                     ASSTETIQF"
     gene            11770..13614
                     /locus_tag="VLE22_09395"
     CDS             11770..13614
                     /locus_tag="VLE22_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HSB14662.1"
                     /translation="MRHRKNRKRGQILVMVTLALFMMAGIMGLAVDLGWSYFTKKSAQ
                     TAADAAALAAAEAAFAAESDEDANSTYDCGTEVACVPLSQCPSPIPNPPTNNIEVGCL
                     YAKQNGFQVQPGGRQNVTMNSGNTPNPPTVPGVTVYYWATARVAEGIPQLFSSMLGNT
                     LNLASARATGAIIDSTIAGSLILLNRERDCVPMNLGSQPICGVDLLVQANNNQGMDAV
                     RADGGVLMASVLNGTNDPSGEGNYSGENQGGGLVRAPFTFIRGDGTVSLGGSSQWIQS
                     PTNGFSDRSYFRDPMRGKGSPPPPAPGLQDIPIMGGALMGSNDPNNPTIYSPGNYYAV
                     KQVGGTLVPTGDPLTMSGYLKFQADSDGFGDWVFFGGLRNQSAGTVMTVDPGRYVIAG
                     VRSNGNNVTPGFDIQVNMSITDNGPPNAAPAPSNAGEIFIFTDPTYLGGPAGDIPLTI
                     PVALQNSPVLNDLKFGISGFQTGNNADIQVNIHGLNRDSGALPAELKDFTPVVMWQDQ
                     QNSVIQYTPRGYIDNSCGNLDPQQGCANSALDNAASTEMFFKASPSLHIYGTVYQPRG
                     AFTSMIGAGGYDSPLMIIAGALMVHANSNVRLQELDSPLYVKMVALVE"
     gene            13616..15322
                     /locus_tag="VLE22_09400"
     CDS             13616..15322
                     /locus_tag="VLE22_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14663.1"
                     /translation="MSENKPNPVIRVLPSLTDVAFVMPVLFLFLRMQGADYLLGDGDT
                     GWHIRTGDWILQNWSVPYRDMFSYTKPGQPWFAWEWLWDVVFAWLHQHLGMGAVLLGS
                     LLVICLTSAMLFRLVRRMSGNVLLAIVTTFIATAASSIHWLARPHLFTLLFVVIFCMV
                     LERAREGRTRILAILPFLTVIWTNLHGGFFVGIVLIGAYAAGELVSWLIETEADVKRG
                     ALRRAKPYVLAAAGCAAASLINPYTYKLHVHMWRFLTGGFHMEHISEYLSISFQHPQA
                     LWFELMIVAGAIAGMWSLYRKRFAHAILIFGWMHLALISSRNIPIFVLVAAPIVAWAL
                     NEFLGRLASAPVAGWLRSAADSVRSIAAEIDETDRVPRLHVASAFAITIIGLLVYAPN
                     APEKFRPEYSKKRYPAKALEALREQDPSLRNVFTHDEWGDYLIYCCYPETKVFIDGRV
                     DFYGPELGKKMLEALNVKYDWEHTLDRYDVETVLLPVDSPLAGALKESRPWRVVYDDG
                     MAIAFRLTEPGRLARHFRTLGQPKASAVACDGGIHRDRKTTKTDNRDPRITQPNSRSE
                     SL"
     gene            15319..15501
                     /locus_tag="VLE22_09405"
     CDS             15319..15501
                     /locus_tag="VLE22_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HSB14664.1"
                     /translation="MKLMKNFIRDEQGQDLVEYTLLLAFVALASAALFIGAGASINTI
                     WKITNNTLSNAAASAS"
     gene            15574..15765
                     /locus_tag="VLE22_09410"
     CDS             15574..15765
                     /locus_tag="VLE22_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14665.1"
                     /translation="MDKSEVGSGFIRDDRGQDLVEYSLLLAFVCLAGAAAYLGVGRSI
                     NTLWTIANSRLAAANESPS"
     gene            15781..16677
                     /gene="cpaB"
                     /locus_tag="VLE22_09415"
     CDS             15781..16677
                     /gene="cpaB"
                     /locus_tag="VLE22_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HSB14666.1"
                     /translation="MSKRLISVLVFAFIVSAGASLLLYRLVSSRMNTEAKQSTAQIMV
                     AARSLPVGTMIGEMDIKMGAWSGPLPVNAVTKKEEAVGRGVIANIYEGEPLLESRLAA
                     KGAGAGLAATIPEGMRAVAIRVNEVVGVAGFVVPGQRVDVLVLGQPPGGGSGGTMAKT
                     ILQNIEVLSAGQNIQKDSEGKPVTVQVVNVLVTPEQAETLSLISNDMRIQLVLRNPLD
                     TKEVKTQGAAAGNVFTGAPTKMPTAEGKAVARPRVARPATPPPPILPRRETVPVVVEV
                     IHGTRRAEQKFKEAEKKDEQNQ"
     gene            16674..>17224
                     /locus_tag="VLE22_09420"
     CDS             16674..>17224
                     /locus_tag="VLE22_09420"
                     /inference="COORDINATES: protein motif:HMM:NF025014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HSB14667.1"
                     /translation="MTRRTGGKTVIATKFPATRLAVLVLTFSTIAVLAQVPQEAPSAA
                     ARDLFVTVGKSLVVDSPLVIERVAVANPDAAEAIAVTPREVLVNGRAPGETSLIIWQQ
                     GGNRLIFDLNVRQSVTALETVRQELQKELAGQDVSMTLENNTVWLRGTTNDLTGADRA
                     VSIASTLGPKVVNLLHVKVPPVDA"
BASE COUNT         3378 a         4877 c         5599 g         3370 t
ORIGIN      
        1 gcgtcattca gatcaggcac aatacccaag agcacttcgc ggtctgcaag cggaaggcct
       61 ttgacgatga cccagaggcc agcccggctg aggtcggtga tctccaactc agggtgggtg
      121 gccttcaagc ccgccagcgc cgcaactgcc tggggcggca aggcccgctc ggagctccaa
      181 acgaacaccc agcgccgcat cctttcctgc caacgggttc ttgcaccctc gaagtcttcc
      241 tcgattttct tcgccgcctc cacccccgtc attttctcgg gtgcatagca ctgatagatc
      301 gtcttgctct ccagagaata gccatctgct ttccagtctc cttccttccc catgggttta
      361 accatgatga atgaggagtc cgccctttgc atgatctgct caaagaagtc ctggaagctt
      421 ttgcctgagc gtcgaaaaag ctcgagctcc agcctgagct cgtggtaatt aaggtcgaat
      481 tctcccttgc gcataggccc aaacaatatt gactcaaatt gtttccgcct gcaagccagc
      541 ccatcgatca ttctgcactc gacttgccga atgagatcga tgccggtggg tttgcgtgca
      601 agggatcgtc gggacgcagt agcagtctgc cttggagata cgccaccggg aagtgtttcg
      661 ttggagcggc tcgccgtctc gacatccctt ggtccccggc aatagccatg gacagttgtt
      721 ttcgagaagc agcatctcga aactgctgtc actgggttct caatcgagaa ccagccattg
      781 tgagtgaatt ggagttggaa aattaggcca cggttatcga cgcagtcgtg gcctaccgct
      841 gtgacggtgg ctttcgagtg actgccggcg gcttggggcc ggagggcggc tcgaaggacg
      901 ccgccagact gccggaccga ccccattcgt caatgatgcc ttccgacagg ccgatgtcat
      961 ggtggagcac gtcacgcagt tcgtccggtg agaggtcccg gcggtcaacg ccttcggcgg
     1021 ttttcgcgaa ccgggtgttt ccgatcagta ctaccgtctc gccggggcgg ttgcggcggg
     1081 cgtagagacg gctgttgaac gggcttcggt tcctcgactg ctcgtagtag tcgagatagc
     1141 cggcgtggct ctcgcggtcg gcgatcaaac ggcagggcag gtagctcgaa ttgggcggcg
     1201 tgtggctcca caccacgtgc gtgccgtctt cgaattccac ctccgccggg atcaccgggt
     1261 cgtcatggat gaacacactc tcatccagag gtagcggtgc gttcgagagg agggaagagt
     1321 ccgcgagcca atctcgattg ccgatcctga ccttgaccgt agcgtgattg acgatgccga
     1381 ggtcgcgcat cgctcccgcg gcccggcacg catcaaatcc cagcgagcga aggagttcga
     1441 acagcgcatt actggtcggc cagcatgttc cgccggttcc ggtggctagc cagtcttcga
     1501 aaaacggacc cgcgtggccg cctggaagaa tcggagatct gtctgcgcga agggcaatca
     1561 tcttccgcac gttgtcgaat gggacgccaa cgcaccatgc acggtagatc gcatgaagcc
     1621 catcgagatc ggctgacgga ggcgccgtga aaccgaggcg cgcgagaacg cggtcgcgga
     1681 gcgcgccttc cagcccggat gacgattcga aacgcgtcaa cgtggatccc ctgcttgacc
     1741 aaatagagta tttattctac gattagagta tatactctaa tcgggagtca acatgaaggt
     1801 ccttctcatt cagccgcaaa gcagcaatcc cctcatggat cgagtgtacc ttttcgagcc
     1861 gcttgcgctg gaatacgtgg gggcgggtct gaagcaggcc ggccatgagg ttcgcctggt
     1921 ggacgcgcga ctggagcccg acgcggagcg cgtgttcacc gagttccggc ctgaagccgt
     1981 cggaatcacc ggattcacga cccacctcaa tatcgtcaag caccttgcgg cgcgcctgaa
     2041 gaaccgagat ccgaaggtgc tgatcgttgt cggcggccac catgcgacgg ttcgtccgga
     2101 tgatttcgcg gggacggccg tggacgtcgt cgtgatcggc gaaggcgtct tcgcaatggt
     2161 ggaactcctc caagcggtcg agcgaggcga ggaacctcta acggtgcgcg ggctcgcgct
     2221 ccaggggccg acaggactca tctataccga accgcgggcg ccctctccgc ttgatgtact
     2281 cccgatgccg gatcgcaacc taacggccgc ccatcgcggc agctacttca gcgaatggtt
     2341 tcgaccgttg gcttcggtgc ggacatcgct cggctgcacc gcccgctgca cattctgtgc
     2401 cctgtggtcg atcaccggcg gaaaatacct gcgccgagat cccgagaatg tggtgagaga
     2461 acttcagacg atcgcggagg cgaacgtatt tttctgcgat gacgaatcta tggtcgacgt
     2521 gaagcggatg agccgccttg ccgaccttat cgccgaaagc ggcatccgaa agaggtattt
     2581 tctttacgcc cgcgtggata ccatcgtgaa gcacccggag ctgttcggga agtggaagcg
     2641 gatcggactg tcccaggtgt tcgtggggat ggaagacttc tccgatgccc gcctcgtagc
     2701 gatgcagaag ggaatctcca cggagcagca agcgatggca gtgaagatcc tgtccgacct
     2761 gggggttatg atgtacgcct cattcatggt ggaccccgct tacgaagtcg aggattttcg
     2821 ccttctgcgg gaatacatcc ggaggctcag gctcacgtac gcgaccttcg cggtactgac
     2881 gccgctcccc ggaaccgagc tacacggctc ggtgaagaac cgtttgctgt cgcagaagcc
     2941 ggagctttac gacatgctgc acgcgctcgt accgacaagg cttccgttgc cggagttcta
     3001 tgagcagatg gcgcggcttt acgccggggc gatcccgctt ttccggtctc ttccggtact
     3061 gttgcggtac ggcctgcacg ggattctcgt ccggctgcgc gtgtacgaga cattccttgc
     3121 gagggttagg gcgtcacatc tcgactactg agaggacgat cgggaagcgc acgggatgaa
     3181 caccagggac aagatcatcg aaacagcgat tcgcctcttc aacgaaaggg gaaccagggc
     3241 ggtcactacg aatcacatct ccgccggagc cgggatcagc cccgggaatc tctactatca
     3301 ctttcgcaac aaggaggaga tcatccgcgc catcttccgg cagatggatg tctacgggcg
     3361 ggaagagcac cgacgcatcc acgagcggac gacgggaacg atcgagacga tcgaagagtc
     3421 tttcgtcatg atccagaagt tcaactggcg gtttcggttc ttcaaacgcg agctgacatc
     3481 gctggtgaac gcggatccgg cactgaagga ggacttcgtc cgtacgcatc gcgagacact
     3541 cgtcttggtg cgttccagca tcgaccgttc catcgagcac gggttgcttc tcagtcttcc
     3601 ggaagaagac cgccagtttc tggccgaggc gatatggctg gtcacgctct tctggcccaa
     3661 ttacctcgag gtgagcgggg aggaagtgaa cgaggcgtcg ttgcagcggg gaagtgattt
     3721 gctccggcgg atactggcgc cgtacgtcgt ccagcggccg aagcgacgga gccggccggg
     3781 ctaatccgcc cgcggctcgg aggaatcgaa gaggacgccc ttgcgcaagc ggagccaggt
     3841 ggggaagagg gacgggtctt ccagccagac ggtcatccag gagatcattt cttgcttgag
     3901 ggactgaact tcggcggagg cgcggcggct gcgcgcggcg aggcgggcgt gttccttcgc
     3961 catgagcacc tcccggcgtg cgcgcgtcgc gagctcgcgg tcgtcgcgtt cggaggcgcg
     4021 ctcgtagatc tggccaagcg cgagcagggt ccgttcgagg tcgtcgtagc tgtcctggcg
     4081 gacgccttct acgagcggtt cgagcggaac gccgcacgaa cggagcagtt tccgcaggta
     4141 ggagtcgctg gtgggcgcca gcagatcgtg gatctgccgc tgttccgcgt caccgattcg
     4201 cgcgggccgg ttttccctca gccactggtc gagcaccgcc cgcctggttg tgttcctgga
     4261 tgccacgtcg atagttcctg acggcgcggt tgtgcgcgtc aagctcgctg gaaaactgat
     4321 gcctgccgga gccgtccgga cgggcgacga agtagaggta cggtgtgtcc gccggttcaa
     4381 gtgcggcctt gagcgactcg agtcccgggt tggcgatggg gccgggagga agcccggagt
     4441 gctgataggt gttgtatcga ttcctgctta gcagatccga gcggaagatc gtgccgcgat
     4501 agcgctcttc gagcatggcc gcgtaaatgg tagtgggatc gcagtcgaga ggcattccga
     4561 tgcgaaggcg gttgtggaac acggaggcga tggcagcccg ctcctcgctg agaccggttt
     4621 ccttctcgac cagcgaggca agcgtgacgg cgcgatggac aggcgcatcg acgccgaggc
     4681 ttctccacgc ctcgcgaaaa cgtgtagtca tcagccggca gatctccgtg gccgtggttt
     4741 ggcgagtgag gcggtaggtg tccggaaaga ggtagccctc gagtgttggc gcctcgggag
     4801 cgagatcgcg aatgagcgag gggtcgcgag cggcttcgag gaagccatcg cgcgggagca
     4861 ggcccagctg gtcgagggaa tcggcgatat cgaaaatgtt attgccctcg gggactcgaa
     4921 gctcatagaa gaaaacgtcg ccgcgggcta tgcggtcgag cacatcgaga gcagtggccg
     4981 gttcccggaa acgatactcg ccggcctgaa gcgtcacacg gggccgcagc gtgcgagcgg
     5041 cgagaaactg ccagcggtag cgtactacgc cggcgccggc cagcagcccg gacagttgca
     5101 ccgtgccggt gcctcgggga atgtcgagga aaacttgatt ctgaaaaccc gaatacggct
     5161 ggcttagcga aaacgcagcg agcccaagaa gacaaaccgc caggagcagg gtgaccacca
     5221 gccacttcat gcctcggcgt tctccgcagc ggtgtcgatc gatcccagat agctctcgag
     5281 gaggatcacg gcggcgaggc ggtcgacggc gcgcccccgc ttctcccggc tgatgccgct
     5341 ttcgcgcagc acgcgctctg cctcgacagt ggtgaagcgt tcgtcccaga ggcggacggg
     5401 aaggcctgtg caggcttcga gtttcgaggc gaactcccgg acccacaccg actgcgcacc
     5461 ctcgacacca ttcaagcgga tgggatttcc gatgacgata agcgagacgc ctcgatccga
     5521 ggcgagctgc gtgagcgccg cgagatcgtc acggaggctg gtccgagcga gggtgtcgag
     5581 tccctgagcc gtgattcgaa gctcatcgct catggcgagt ccgatgcggc gccgtccgag
     5641 atcgagcgcg agaattcgcc caggatactg aagattgttt tcgttcactg aattgatttc
     5701 attatagggc ggctcgtgtt agagtagcgt tgagcgaagt tttcggtggg attctctcgt
     5761 ggccgttctt gccagctcca tccgcaccct gaggccgaaa aacgcagctt ccgccgttct
     5821 tgctgccgcg gcgattattg ggttgctgta ctacggcagg gtattcttca tcaccctgat
     5881 cattgcgatt acgatcgcct ttatcctcga accgtttgtg agtttcgcga tgaggctacg
     5941 gctgccgagg gcggtcgcaa gctttgtggt ttgcgccctg gcgctgctgg ttctttactt
     6001 ggcggggctg ggcttctaca ctcaattcgc tgacctttac cgcgaactgc cgaagtacag
     6061 ccagcggatc ggtgagctga cggaggtggt gacccaaaag gtggaggaga tggaacgttc
     6121 cacctaccgg ttgctcctgc cccggcggat gcagcgtcag gaacagcagc ccgcgcaagg
     6181 ggaaccgccg cgggcggcgg ccaagacgca accacggcgg cgatcggccg agccgcccat
     6241 gcccatcggg cctcctcccg tgcaggaggt ccggattctt tcggacgagc ggacgccagt
     6301 tgtgaactac ctttacacgc acctcgggct ggtttacgaa gtgctgctga tggcgtcatt
     6361 cgtcccgttc ctggtctact tcatgctgag ctggcgggac cacatttacc ggagttttct
     6421 gcaattgttc gagggccagg acaggttggt ggcgggcaac agcctggagg ggatcgcaag
     6481 catggtgcgg gggtttgtag tgggaaactt cgtactcgga gtgctgctgg cgtcggcgag
     6541 ctcgttgttg ttctgggcgt ttaatttacc ctatccgctg ctgatcggtc cgctgagcgg
     6601 gtttctgagc ctggtaccgt acgtcgggct cccgcttgcg atggttccgc cgctgttcgg
     6661 cgcgctgacg gtgctcaaga cggcgcccgc gtacctgctt ctcgcgacgc tcgtatcggt
     6721 gcttcacctg ctcgcgttga acctgttcta tccgaaggtt gtcggctccc gcgtgcatct
     6781 gaacccattg gtcgtgacgg tggcgctgat ggtgtggggc gtgctttggg gagcggcggg
     6841 gctggtgctg gcgatcccga tgacggctgg catcaaggca gtgtgtgaca acgtgacaag
     6901 tcttcagccg tacgggcggt tgatgggaga ttgaagggtt gacgcggagg gattctcttg
     6961 ctgaagaaac agtttctgac gtacgtcgga atcggactgg tgctggtagc gatcggcgtg
     7021 gcggtcattt tccatattca gcgtggagcg cacatcgagc taagaggcag cgtgctgaag
     7081 gtccgcaccc ttccaatcga caatgccagt gccatcgcgg ttatcgattt ccggtttgtg
     7141 aatcccgcgg actacccgtt tgttgtccgg cgcgtgaatg tgtcgatgga ggaccccgcc
     7201 ggccgggtca tcgaggggtc ggtgatctcg gaatcggatg cggtgcggct gtttcagtac
     7261 tacccggttt tggggcagaa gttcaacgaa tcgctgacga tccggacgaa ggttgcgccc
     7321 cggcaagcga tggaccgcat gatcggggcg cgcttcgaac tgtcagacaa ggaactcgaa
     7381 gggcgcaaga ggctaacgat ccggattgaa gatgtggacg gggcgatctc ggacgtggtc
     7441 gaagaggcga ggccgtaggg cctcatcggg tgcgcgaaaa actgttgtcg ccttagcctt
     7501 cgtgcggagg aatggcgagc aaaacctcaa cgagcactgg tatgccgtca cgggtggcgg
     7561 gccggaagac ccattcggtc aactggggga tcagaagctc gcggatctgg tcggctggcg
     7621 cacgtaacac attcatatcc ttgaacttgc cggctttgtc gacaaatccg tgaaccatga
     7681 tgtagctgga gcggggcatc atcgttgccg gagggaggac cgtgaccagc gggaacggag
     7741 ccttcacagg ggtggctttg ccgatgtaga cagcgccgcc gacgactttt gtgacgggtc
     7801 cggtgtcctt cgggatgcag tactgaagga gccaggctct cggagctccc acctggaggt
     7861 ataccgtata gaccggctgt ccgctaagca cacccgcgct ttcggggaag ccttcgtccg
     7921 cggaagattg catgacaacg acgtcgaact gagcgttgga gggatgctcg atccgaatcg
     7981 ccgaggcgta cttcaggccg ggagccaagc caccggtccc ggccacgcca ccggctccag
     8041 caccagttcc ggttccagtt ccagttccag ttccagttcc agttccggtc ccggtcccgc
     8101 tccctgccga agcggtgccg attcctccag ggccgtctcc cagtccggtg ccactgcccc
     8161 caagcccgct gccgagggaa gccaacgtgc cggttccgcc cgcctttgag ccgggaatac
     8221 cgggtcggcc gagggcgtca tccgcgccgg gcggaggcgg cgggatcctc ccgatctggt
     8281 tgccgagagg gatcgaaatc tgatctttca actggcccgg gttagacgaa agcgcaagca
     8341 cgttgatcgg ctccccggag atcgggctga ccgaggcgcc ctggccggac gccgtggaga
     8401 ccggcccctt gaacggacgg ccctggatgg tgggtctcgg taagtgcaat aaggcgttct
     8461 gcggacccgc aaccatggac ggcacgtaaa gatcggaggt cgtgctgccg gagcccgcca
     8521 aagcaggcgg ggcgtcgacc cgagggatga ccaccgacgg ggcgctcggt cccggctcgg
     8581 cgaacttctt cgccgcgggt cggggcaagg tcggggcgga cagcaggata ttcggcaatt
     8641 gcaccttggc cgtcagttgc agttcgggcg gcaggtgcgg ctgaatgagg gtctggtctg
     8701 cctcgggccg gtgcgtgacg ttgggaacac ggaactgctt cggcaggacg cgatcgggtt
     8761 ccgccttcgg aagaggagcg ggtgcgggcg aggccgccct tgccatctgc tgggatggcg
     8821 ctttgagccc gcgtttctcc tccggtttgg gcttctccac caccggcctg ggtgcgatcg
     8881 cgacctgggt cgaacttagg tagaggcggt cgggtatctg aatttcgagg gggcgaaacg
     8941 tcctcgcgtg gcgccgccaa acctcctctt ccgagtcaga gagcatcgac gtgagcggca
     9001 gaaggacaac gatgacgaag acgtgaaaga gagctgaggc ggcaaagctc ggccaatggg
     9061 cgcggggcgg gcggaaaacg tcctcgaaga gctggtgctc ctgcaccggc ggagacttta
     9121 cggacgccag cctgctcgcg accaatccca gtaccgaaat atcctgcatg cgattccccg
     9181 acgcccggcc catggggcca ttatcccctg tcggtctacg tttagtcgaa taccgtccca
     9241 ggaccgcgaa ccttcagttg cggcttctca acaacaagtg agcggagcca ctgttcctgg
     9301 ttgacatctt tgcgcggcgg ttgcactgtt ctcggctggg gcatctcgac aagagcttct
     9361 ccggccggca tgttcttgag accgtaaagc gccagttcaa agcggtcctt caccccaagc
     9421 ttgtggaaca gccgggacaa atacacctta acggttcctt cggagataaa cagtgtagcg
     9481 gcgatctctt tgttctttag tccctgagag aggaggctga caagctggct ctcgcgacgc
     9541 gtcagagaga tcgtgcggac gccgtcgaag cttgccttga gcgtttcctc gaaccagagg
     9601 ccgccgtcag cgaccatttg aaggcacttg aagagcgtct ccggatccag ggtcttgcga
     9661 aggattcctc ggatgccgtg ctcgatggct tgataggcga gttcggtgga aatcgcgcgc
     9721 acccaaagga cgaccttgat cgcagggaac cgccgctgaa attcgaccag cgagccgaaa
     9781 gtgaggtcgg accggagatc gagaagcagg agatccggcc gcttggcctc gatttcagct
     9841 acaagctggg tcgtggtttc acaaaccgcg tccaacgtga agccaccagc ggtggagagg
     9901 atggacctaa gcccggcggc aagaacaggc tcgtccgtga ggaggtagag gcgagtcata
     9961 gcactccctt ccgagaggcg ggaccgcgca cgctctctcg acttcgtgac gttgcacccg
    10021 ggatgcccgg ccagagtacc agcaacgaat aaacggataa cgataccact tatccgggaa
    10081 tgggcgcatg ggcaaccaat ataacacgga aaaaacggaa attccagatt ttttcagatc
    10141 aatttcgccg ttttttgata gttttgcctg attgacatgg cgcatagtac ttccccgcgc
    10201 ctagtttcat aacttcggat ctcatggtct gatggcgggt gccggaatag gtattaacct
    10261 tagtaaacaa agagatgcta cccatttacg gtcttgaaga ttcggccgta aacccgtaca
    10321 ttgacaccac agatggagag acgtcgcagt tcgaggttgg atatcagcct gcaatgtcgg
    10381 gtctcgtcgc cgcgaatcgg ctcgcgagtg ctgatgggaa tcacggagaa catgagccgg
    10441 ggcgacgttt tggtccttct ggacggggag caggcgcttc ccgaattgcc gtgtgtcggc
    10501 gatccgctga ttgtcgagat tgaattgccg gaaaatcacg cgttcggccg caagtgcatg
    10561 cagtgccaga ccacggtggt gcgggtttct caatcggagg agggtgtgcc cagggtggcg
    10621 ctgcggatcc acaagatgcg ctttcagagt tgcggaaagg actcggtgca actcataggt
    10681 cagcggacgg gtgtccggca acttctgatg taggcggcag cgagggcgcg ccggggagca
    10741 acaaaaccga tgaaagacaa aaccgataag cgagttcgga agaccgcgaa gaaggaatcc
    10801 gggcacgcgg tgattgaggc gtcgctgttg gcgccttgga ttttcttttt gtttattggg
    10861 gtttttgatt tcgggtttta ttcttacgcg gcgatcgcca cgcagaacgc ggcacggatt
    10921 gccgcgatgc agacgtcgga caacacgaag acggcagcag acgacgtctt ggcgtgccag
    10981 tacgccgtac aggagctaca gatgctgccg gggataccga aggtaacgac cctggcgaac
    11041 acgccagcct gcccgacagc gacgggcggg atcacaaaca cgtttccgtt gtcggtggac
    11101 gcacagagcg tgacgggacc ggacggcgcg ccggcgacgc gagtcgcggt tacgtaccga
    11161 acggtccaac tggtgccgat cccgggtctg atgaccggcc gcctgcagtt gacccgggtc
    11221 gtggaaatgc ggctaaagga gtcctaggcc atgccagatt gcaagaacaa acgccgcggc
    11281 gacgtcatgc tggagttcac gctagtcgga attccgctga tcttcatcct gatttccgtc
    11341 gtggagatgg cgcgagggat gtggacgtat cacacgctgg cacacgcggt gaaggaaggg
    11401 acgcggtacg cgatcgtaca cggctacaat tgctcgctcg cgccaaacaa ctgccaggtg
    11461 acggtggcac agatcgcaca gcgggttcga gaggccggcg tcgggttgat gccaaacgag
    11521 ctgcggatcg agctgcggtc ggaaggcgac acggtgctgt gttcgacgct cgacaactgt
    11581 cttacgaaca acaacttctg gcctgtgtac ccgaacagtc agattgggca aagggtcacg
    11641 atcaatggga cgtatccgtt ccggtcggca atagcgatgt tttggccggg agcaggcgga
    11701 ggcatgaact tcggcacctt cctgctgccg gcatcgtcga cggagacgat ccagttctga
    11761 ggaggcgcca tgaggcacag gaagaacagg aaacgcggtc agatcctggt catggttacg
    11821 ctggcgctat tcatgatggc gggcatcatg ggactggctg tggacctggg ctggtcgtac
    11881 tttacgaaga agtcggcgca gacggcggcg gacgcggccg ctctagcggc ggcagaagca
    11941 gcgttcgcgg cggagtcgga cgaagacgcc aactcgacgt acgactgcgg caccgaagtg
    12001 gcatgcgttc ctctctcaca gtgtccgtct ccgatcccga acccgccgac aaacaacatc
    12061 gaagtaggct gcctctacgc gaagcagaac gggtttcagg tgcagccggg aggtcggcaa
    12121 aacgtcacca tgaattccgg caacacgccg aatccgccca cggtgccggg cgtcacggtc
    12181 tattattggg cgacggcgcg ggtggcggag ggaattccgc agcttttctc gtcaatgctt
    12241 ggcaacacgc ttaaccttgc gagcgccagg gcaaccgggg caattatcga ctcaactatt
    12301 gccggctcgc tgattttgct gaatcgtgag cgagactgcg tgccaatgaa cctcggctcc
    12361 caaccgatct gcggcgtcga ccttctggtg caggcgaaca acaaccaagg aatggacgcg
    12421 gtgcgggcgg acggtggggt gctgatggcg tcagtcctga acgggacgaa cgacccgagc
    12481 ggagaaggaa actactcggg tgagaatcag ggcgggggcc tcgtgcgtgc gcccttcacg
    12541 ttcatccgcg gggacggcac ggtcagtttg ggcggaagct cgcagtggat tcaatctccg
    12601 accaacggat tctcggaccg ctcatacttc cgcgatccaa tgcggggcaa aggatcgccg
    12661 ccgccgccgg ctccgggtct gcaggacata ccgatcatgg gtggggcttt gatgggcagc
    12721 aacgatccga acaacccgac gatttacagc ccagggaact actacgcggt gaaacaggtc
    12781 ggcgggacgc tggtgccgac cggtgatcca ctgacgatgt cgggttatct gaagttccaa
    12841 gcggacagcg acggatttgg tgattgggtg ttctttggcg gactgcggaa ccagagcgca
    12901 ggaaccgtga tgacggtgga tcccggccgt tatgtaatag cgggagtgcg gtcgaacggc
    12961 aacaacgtaa caccagggtt cgacatccag gtaaacatgt cgatcaccga caacggaccg
    13021 ccgaatgctg cgccagcacc ctcgaacgcg ggcgagatct tcattttcac ggatccgacc
    13081 tacctgggcg gacctgccgg agatatcccc ctgacgattc ccgtggcctt gcagaacagc
    13141 ccggtgctca acgatttgaa gttcgggatc tcgggtttcc agaccgggaa caacgcggat
    13201 attcaggtca acatccacgg actgaaccgg gatagcggcg ctcttccggc ggaactgaag
    13261 gatttcaccc cggtggtgat gtggcaggac cagcagaatt cggtgatcca gtacacgccg
    13321 cgaggctaca tcgacaactc ctgcggaaac cttgatccac agcagggctg cgcgaactcc
    13381 gctctcgaca acgccgcttc gaccgagatg ttcttcaagg cgtcgccgag ccttcacatc
    13441 tatggaacgg tgtaccagcc gcgcggggca ttcacgtcga tgatcggggc gggcggatat
    13501 gacagcccgc tgatgatcat cgcaggcgca ctgatggtgc acgccaactc gaatgtgcgg
    13561 ctccaggaat tagactcgcc gctgtacgtg aaaatggtgg ccctcgtcga atagcatgag
    13621 cgagaacaag cccaatccgg ttatccgagt gctgccgtcg ctcacggacg tggcgttcgt
    13681 aatgccggtg ttgttcttgt ttctccggat gcagggcgcc gactaccttt tgggggacgg
    13741 cgacacgggc tggcacatcc gcaccggcga ctggattctg cagaactgga gtgtcccata
    13801 ccgcgacatg ttttcgtaca ccaagccggg ccagccgtgg tttgcctggg agtggctgtg
    13861 ggacgtcgtg ttcgcgtggc tgcaccagca tctgggaatg ggggccgtct tgctggggag
    13921 cctgctggta atctgtctca cgtccgccat gttgttccgg ctggtacggc ggatgagcgg
    13981 aaacgtgctg ctggcgatcg tcacaacgtt tattgcgacc gctgcgtcgt cgatccactg
    14041 gctggcgcgg ccgcacctgt tcaccctgct gttcgttgtg atcttctgca tggtcctgga
    14101 gagagcgcgg gaaggaagga ctcggattct cgctattctg ccattcctga ccgtgatctg
    14161 gaccaacctg catggggggt tcttcgtcgg catcgttctg attggcgcat atgcggcggg
    14221 cgaactcgtc tcgtggctga tcgagacgga ggcggatgtg aaacgggggg ccctgcgacg
    14281 cgccaaaccc tatgtcctgg cggcggccgg ctgcgctgct gcgagcctga tcaatccgta
    14341 tacgtacaag ctgcacgtgc acatgtggcg ctttctgacc ggcggtttcc acatggagca
    14401 cataagcgag tatctgtcga tcagcttcca gcatccgcag gcgctctggt tcgaactgat
    14461 gatcgtggcc ggtgcgatcg cgggaatgtg gagtctttac cgcaagcgat ttgcgcacgc
    14521 gattctgatc ttcgggtgga tgcatctcgc gctgatatct tcccgcaaca ttcccatctt
    14581 cgtgctggta gcggcgccga tcgttgcctg ggctctcaac gagtttctgg gccggctggc
    14641 atcggccccg gtggctggct ggcttcgtag tgccgcggat tcggtgcggt cgattgccgc
    14701 cgagatcgac gagacggacc gcgtgccacg gcttcacgtg gcgagtgcgt tcgcgatcac
    14761 gatcatcgga ttgctcgttt atgcgccgaa tgcgccggag aagttccggc cggagtacag
    14821 caagaaacga tatccggcga aagcgctgga ggccttgcgc gagcaggatc cttctttacg
    14881 gaacgttttc acccacgacg agtggggcga ctatctgatt tactgctgct atcccgagac
    14941 gaaagtcttt atcgacggca gggtcgattt ctatggcccc gaactgggca agaagatgct
    15001 cgaggccctg aacgtgaagt acgattggga acacactctg gaccgttacg acgtcgagac
    15061 agtcttgctt cccgtcgatt ctccactggc tggagcgctg aaagaatcgc gcccctggcg
    15121 cgtcgtgtat gatgacggca tggcaattgc cttccggctg acagaaccgg gccggctggc
    15181 gcgccacttt cgtaccttgg ggcagccaaa ggcttccgcc gtcgcctgtg acggcggaat
    15241 tcatcgtgat cgcaagacca cgaaaaccga caaccgtgat ccgcggatca ctcaacccaa
    15301 ttcgaggagt gaatcactgt gaaactgatg aagaacttca tccgtgacga gcaaggccag
    15361 gacctagtcg agtatacctt gctcttggcg ttcgttgccc tggcttcggc cgcgctgttc
    15421 attggcgccg gcgccagcat caacaccatt tggaagatca ccaacaacac gttgagcaac
    15481 gcggcagctt ccgccagcta gacgtgttcc ttccgggggg catgcttcgc gtgccccccg
    15541 ctgcttattt ttcaacgtta ggagagaacg gcgatggaca agtcggaggt cgggagcggg
    15601 ttcatccggg acgaccgagg ccaggatctg gtcgaatatt cccttctcct ggcattcgtc
    15661 tgcctggcgg gagcggcggc ctacctggga gtcggcagaa gcatcaacac gctttggacc
    15721 atagcgaact cccgtttggc ggctgccaat gaatcgccga gttgagagag ggaaagtcga
    15781 atgagcaaac ggttaatttc cgttctcgtt ttcgcgttca tcgtatccgc gggggccagc
    15841 cttctgttgt acaggctcgt gtcgtcacgg atgaacaccg aagcgaagca atccacggct
    15901 cagatcatgg ttgcggcgcg aagcctgccg gtcggcacga tgattggcga gatggacatc
    15961 aagatggggg cgtggtcggg tcccctgcca gtgaacgcgg tcacgaagaa ggaagaggcg
    16021 gtgggccgcg gcgtgatcgc taacatttat gagggtgagc cgctgctcga gagccggctt
    16081 gcggcgaagg gagccggggc aggcctggca gcgacgattc ccgagggcat gcgcgcggta
    16141 gccattcgcg tgaatgaggt tgtcggcgtt gctggtttcg tagttcccgg acaaagagtc
    16201 gacgtacttg tactcgggca gccgccggga ggcggtagcg gcggaaccat ggccaagacg
    16261 attctgcaaa acatcgaggt gttgtcggcc ggccagaaca tccagaagga ctcggaaggc
    16321 aagccggtca ccgtgcaggt cgtcaatgtg ctggtgacgc cagagcaggc ggagacgctg
    16381 agcctgatca gcaacgacat gcgaatccag ctcgtgctga gaaacccgct cgacaccaag
    16441 gaggtaaaaa cccagggcgc cgcggcgggt aatgtgttca ctggggcgcc tacgaagatg
    16501 cccacagccg agggaaaggc agtcgcgcga cccagggtgg ctcggcctgc gacgcctcca
    16561 ccgccaattc tgccgaggcg ggagaccgtg cccgtcgttg tcgaggtgat tcacggtacc
    16621 cggcgggccg agcagaagtt caaagaggca gagaaaaaag acgagcagaa tcaatgacaa
    16681 gacgaaccgg aggaaagaca gtgattgcga ccaagttccc cgcaaccaga ctggctgttc
    16741 tcgtcctgac gttttcgacg atcgctgtgt tggcccaagt accgcaagag gccccgtcgg
    16801 cggctgcccg cgatttgttt gtgaccgtag gcaagtcgct ggtggtggac agccctcttg
    16861 tgatcgagcg cgtggcagtg gcgaaccctg acgcggcgga ggccatcgcg gtcaccccgc
    16921 gcgaagtgct ggtcaatggc agggctcccg gcgagacaag tctcatcatc tggcaacaag
    16981 gcggcaaccg tctgattttc gatttgaatg tccggcagag cgtcacagcg ctggaaaccg
    17041 tgcgtcagga acttcagaaa gaactggccg ggcaggatgt gagcatgacc ctcgagaaca
    17101 atacggtgtg gttgcgcggc acgacaaacg acctgaccgg cgccgatcgc gcggtatcga
    17161 ttgcgagtac gctggggccg aaagtagtga acctgctgca cgtgaaggtg ccacccgtgg
    17221 atgc
//
LOCUS       DATIFQ010000205         2583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_834765, whole genome shotgun sequence.
ACCESSION   DATIFQ010000205 DATIFQ010000000
VERSION     DATIFQ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834765"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1377)
                     /locus_tag="VLE22_09425"
     CDS             complement(301..1377)
                     /locus_tag="VLE22_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14668.1"
                     /translation="MDRLASCRCLKIIALFCLLMSLPVAVPLRSVLAASQPDTFQHQL
                     DQGRALLRQGDLKAAEEAARKAIQLNPSSAEAHHLLGQILFKARKPNEAGEALATAVK
                     LKPAYAEALNDLAEVYVVQGKSAEAEQALTRAIEVSPKHAESYLDLAKLYERRPDPVA
                     AKKTYQNLLAVAPNQPDALFELAMLYDNQGETKTARDTLSRLTKAHPKHANGWYLTGR
                     IAERNNDLVEAAYAYKQAIAAKPDLVDAHYNLGFIYRSQGKLNEAEREFLEVLRYRPE
                     YAEAHMNLGVVYTSVNKLDDAEHAYERAVALKPDYAEAHYNLGVFYELHRKDMPRALA
                     QYRKYRELGGRDDRVERIVGSTFR"
     gene            complement(1520..>2583)
                     /locus_tag="VLE22_09430"
     CDS             complement(1520..>2583)
                     /locus_tag="VLE22_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14669.1"
                     /translation="PGANPPTVVATLCLLADGHKVAAFSSGAGIPKRVFISSTTAGEI
                     AVVGNDPAVAATYQKLIARIDLCDAAKEATNPVPAVCNDESATPLTTPFTVNNANPHG
                     IRFSKLTGKVYSIQENYRTIVEIDPNPAVPGQWVTNVFDLSTTPYTAFGMSPDGRFLL
                     LRGGSTVAPVTTVLGVIDLGTVGNPLTNFALPVLDGASPSSFKFSPDGKRFYILAGNA
                     AAATKRDRLFAFDSSALIPVAPATEPALTLLPGGEIFLLATGGHSMDVLAMGAAGAGE
                     AKYIIVSNSTANSVSIISAADNTIKQNVTVGLTPGGVTVYYPGAAAAGNQAISSLTGG
                     SASPVAELPERLDDHGMPE"
BASE COUNT          541 a          775 c          733 g          534 t
ORIGIN      
        1 cggtaactca tgctcctcgc tgccgctctc tcacagacat tcttggcggc ctcagggata
       61 agagtgccgg cgcggtagcg ttcccgcccg atgaaaccga ctgtaggtgc ccgatcgtgg
      121 gaatcaagaa actggattcg cgggcgagac ctgatcaggc cccgcacgga gcctggactc
      181 tcttggcgag cgagatatgc gggactcgcg cgaagggcga gaagagccac agcccccgcg
      241 acatcctctt gagaccgcga catgtcatgc aagtcgcgcc aaccccactc ctcgcactct
      301 ttaccggaac gttgatccta cgatccgctc gactcgatca tcacggccac ccaactctcg
      361 atacttccgg tactgcgcca gagcgcgcgg catatctttc cggtgcagtt cgtagaatac
      421 gccgagattg tagtgggcct ccgcgtaatc cggcttgagc gccaccgccc tttcatacgc
      481 gtgttcggcg tcgtcgagct tgttgacgct cgtatacacc acgccaagat tcatgtgcgc
      541 ttccgcatac tccggccgat agcgcaggac ttcgagaaat tcccgctccg cctcgttcaa
      601 ctttccctga ctccggtaaa tgaatccgag attgtaatgg gcatcgacca gatcgggctt
      661 ggcggcaatg gcctgcttat acgcataggc cgcttcgacg agatcattgt tcctctcggc
      721 gatccgtccg gtcagatacc agccattcgc gtgcttggga tgcgccttgg tgagacggct
      781 caaggtgtcc cgcgccgttt tggtttcccc ttggttatcg tagagcatcg ccagttcaaa
      841 cagcgcatcg ggctggttcg gagcgaccgc cagcagattc tgatacgtct tcttcgcagc
      901 caccggatcc ggccgacgct catagagctt cgccagatcg agatatgact cggcgtgctt
      961 cgggctgacc tcaatcgcgc gcgtcagcgc ctgctcggcc tcggctgatt tgccctgaac
     1021 cacgtagact tccgccaggt cgttcagcgc ctcggcgtac gccggcttca gtttgacggc
     1081 agttgcaagg gcctctccgg cctcgttcgg cttccgggct ttgaacagaa tctgacctaa
     1141 taagtggtgt gcttcggcgg acgagggatt cagctggatg gctttcctgg ccgcctcctc
     1201 ggcagctttc agatctccct ggcggagcaa ggcccgcccc tggtcgagtt gatgctggaa
     1261 cgtatcgggc tgagatgcgg ccaatacaga acggagcggc acagcaaccg gaaggctcat
     1321 gaggaggcag aatagcgcga tgattttcag acatcggcag gaagccaacc gatccatcct
     1381 tcctcccagg aagagttcgt gagcggacta taaacggcgg ggaatgacga aatcaatcag
     1441 acacagcccc ggatgtacca gaggcggcag acaatgattg tctgccgcct ctgattttcg
     1501 tcagaacctt ttgcagaagc tactccggca tgccgtggtc gtccagacgc tcaggcaact
     1561 ctgccactgg ggaagccgaa ccacctgtta acgatgaaat tgcctggttc ccggctgcag
     1621 cggcaccggg atagtacacc gtgactccac cgggggttag accaactgtc acgttttgct
     1681 taatggtgtt gtccgctgca ctgatgattg atacagagtt cgccgttgaa ttggagacga
     1741 tgatgtactt cgcctcaccc gctcctgccg ctcccatagc cagtacatcc atgctatgac
     1801 caccggtagc caggaggaaa atctctccgc cggggagaag agtcaaagcc ggttctgtcg
     1861 caggcgccac cggaatgagc gcagaagaat caaacgcaaa cagccggtct cttttggttg
     1921 cagccgcggc attccccgcc aggatataga accgctttcc atccggggag aatttgaacg
     1981 aactaggtga agcaccgtcc aacaccggaa gagcgaaatt cgtgagcgga ttcccgaccg
     2041 tacctaaatc gataacgccg agcacggttg tgactggtgc tactgtgctg ccaccgcgca
     2101 agagcagaaa acggccgtcc gggctcatgc cgaaagcggt atacggcgtg gtgctcaagt
     2161 caaacacgtt cgtaacccac tgaccaggta ctgcaggatt gggatctatc tcaacgatcg
     2221 tgcggtagtt ctcctgaatg ctatagacct ttcccgtgag tttcgagaag cgaatgccgt
     2281 ggggattcgc gttgttaacg gtgaacggcg tcgtcaacgg tgtcgcgctc tcgtcgttgc
     2341 atacagccgg caccggattg gtggcctcct tggccgcatc gcagagatca atcctggcaa
     2401 taagcttctg ataagtggcc gcaacggccg gatcgttacc gaccacggca atctcaccag
     2461 cggtagtgct ggaaataaac acccgcttcg ggatccccgc cccggatgaa aacgcagcca
     2521 ccttgtgccc atcggcaagg agacagagtg tcgcaacgac ggtcggcgga ttagcaccgg
     2581 gac
//
LOCUS       DATIFQ010000206        54971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1792145, whole genome shotgun sequence.
ACCESSION   DATIFQ010000206 DATIFQ010000000
VERSION     DATIFQ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 54971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54971
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792145"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VLE22_09435"
     CDS             <1..271
                     /locus_tag="VLE22_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14670.1"
                     /translation="VTRLFRKLDETARRGFVISDLVRSRAAHAGVWLATHLLSRNRLI
                     RHDGPLSVRRAYTVGELNELARAAGLSDLRFIHRPLFRLVGVLCK"
     gene            268..1482
                     /locus_tag="VLE22_09440"
     CDS             268..1482
                     /locus_tag="VLE22_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HSB14671.1"
                     /translation="MSMAGGGGTDLDPGQLWDAIVAGAGPAGSTTAALLAERGFRVLL
                     VDRATFPRDKMCGEYLGAGCVELLAGFGVELRDTHPIRAMRVMAPDGTVFTAPYPEGR
                     NGLAIRRSQLDALLLENARWKGALCLEGFRVERLLLKDGKVDGIEGRLHGGEEKQFRA
                     RIVIGAEGRNSVVARHLGLFRWNRSHRRTALGIHYEGVLPVSGFAEVFLGAGLYGILN
                     PLGAGEANLSLVVAHDGSLSKDRAGAYFAEALGRLPALQERLAQARPLEEVKVLGPMA
                     HHAARASADGALLAGDAAGFYDPFTGEGVYMALISARLAANVAGEALRRRDCSSRFLR
                     AYDRERSAALGGRYRLEAAIQRIIRHPRAADLVSRRLREREDAASCLMEVLGGLAWPR
                     ALLSGGFLSAFL"
     gene            1479..3275
                     /locus_tag="VLE22_09445"
     CDS             1479..3275
                     /locus_tag="VLE22_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB14672.1"
                     /translation="MIPLLFLLLWPAAAPDTEAEKIQPRRETIVVTGSFEPVPLEEAD
                     RSVDKLDVEGHPLLLRNIAEVLRLIPSVDLRERAPGGVQADVSIRGGTFGQTLVLLDG
                     LRMNDIQSAHHNMDLPVPLESAAAVEVLRGSGSALYGSDAVGGVVNIVTRKPEAGEVR
                     LRAGVGNFGVNAQSISIAGVKGRVSQHLALSRDFSSGFAPNRDYRSLSLFSRLYLESA
                     WGSSTVLLAHGDRPFGAQNFYGNFPSWERTKTWFAALRQDLGARTEASFAFRRHTDLF
                     VLYRDRPQVFTNRHAVESYQGALRRREEAGRNTQIHFGAEVLGDTITSNNLGHHQRAR
                     GAAYAALDMRAVRRFSFSLAAREEIYGPLRSQFSPTAAAGAWLSQRIKLRASASRAFR
                     LPSYTDLYYHDPANLGSPDLRPETAWSYDAGVDWNAGGKLSGEVTVFHRRERDGIDYV
                     RSTPAELWRATNIQRLNFTGAEVAVIAGLGGSHRLDFRYTGLRGAQKALAGLESRYVF
                     QYPVHAGVASWMGSLPGGLAGRVQLGATRRYASDPYAVLDVTVARTLGRVRPFLQFAN
                     LADAGYEEIRGVPMPGRAVMGGVAVILSGGGK"
     gene            3305..4786
                     /locus_tag="VLE22_09450"
     CDS             3305..4786
                     /locus_tag="VLE22_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14673.1"
                     /translation="MIRFLLAAILPLAYCGVGVAADQGQLDASPTLFSVLAAINAAGY
                     DADIDSAANHPFREIVRKELASRNIPCLEDLKRFFSEHRQKDETAELSQYVSFALVVD
                     EAPGFNYRLHQNELPPDVVPLEGFEKLMARFHKEAGIDELWRRAQPEFEAVIARYHEP
                     VTRAVTEANAYLRSATSGYLGRRFQIYVDLLGAPNQIQSRSYADEYFIVLTPSPEPQV
                     EDVRHAYLHYLLDPLASKFAEEILKKKDLGDYAQGAPALEEYYKSDFLLLTTESLIKA
                     VEARLAPRGNRQALVDQAFKEGFILTPHFAEQLPVFESQEQAMRLYFPELAKSIDLKK
                     EERRLESVEFLAERPVRKAKVVPAERKIELTGANKTLEDAEEAYRGRELAAAKERYLR
                     VLQETSEKPLHAKAYYGLARIAALEKDPELSERLFLKTLELAPEPQVKAWTLVYLGRL
                     ADAAGEREQAGKHYQAAIAVAGASAAARKAAEQGLQESFRKDK"
     gene            4846..5670
                     /locus_tag="VLE22_09455"
     CDS             4846..5670
                     /locus_tag="VLE22_09455"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14674.1"
                     /translation="MRNGAVLRFVLGVACATGMLLGQGAKQLTVKSKGEAEAVMALQT
                     ALQSQSPDGIITASDNLLNKYADTDFKVIALYYSAEAYGMKNDFDKMIVYAERTLQAD
                     PKHYQAMLLLAGAIAQRTREHDLDKEEKLKSVEKYVQQADEAVKTAVKPNPNLTDEQW
                     EGLKKGFEAQGLEALGLASLARAQNDKAIEQFKKAVEIDPTPNPSTMLRLAVAYTKAG
                     KPDESIAVLDKVLATPNLHPQIKQIAQAEKARATQAKSGGAAPANNAPPQIEVKKP"
     gene            5687..6403
                     /gene="rnc"
                     /locus_tag="VLE22_09460"
     CDS             5687..6403
                     /gene="rnc"
                     /locus_tag="VLE22_09460"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HSB14675.1"
                     /translation="MRAEPDVLEEAIGHGFHDRDILSRALTHKSFVHEKFAAGEATLG
                     DNEQMEFLGDAILGFLVSEALIRQHPGLPEGQLSKLKAHLVSASHLHEVAQELRLGDY
                     LLLGRGEELSGGRDKKALLANAVEALIAAIYLDGGMEATRQFVLGHVAGGVEALSQGP
                     ALPIIDFKSALQELAQARRLPAPRYAIIKERGPEHAKTFTVEVRIGHDLTGQAEALSK
                     KSAGQKAAQVLLERLSGDDS"
     gene            complement(6412..6828)
                     /locus_tag="VLE22_09465"
     CDS             complement(6412..6828)
                     /locus_tag="VLE22_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSB14676.1"
                     /translation="MTVVARANMADLPLDDNTPQIPDRLYFRIGEVSQLVGVPPHVLR
                     FWEKSFPALSPKKSGTGRRLYRRKDVELLLEVKRLLYERRYTIEGARKYLESRPRRAV
                     QKPAQESSQATLFAAPPALESIRHELAEILALLKRR"
     gene            6941..7807
                     /locus_tag="VLE22_09470"
     CDS             6941..7807
                     /locus_tag="VLE22_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14677.1"
                     /translation="MRRMYFIGVTTAASSIHALFPKWTELAGIEGSELVGLDIEPGAP
                     PQAYREAMRRLRDDTAAWGALVTTHKTAVYAHARDLFDGFDADAEALREVSCIVRREG
                     RMTGVAIDTLTSGLALRQVTGNGPFQGQALIMGAGGAAVALAVHLSRRHRPSRVILTD
                     VSAGRIVEASELAPAECMLVNVPEDHDRILGAMAEGVLVVNATGMGKDRPGCPITVQA
                     RFPAGSVAWDFNYRGDLLFLENARAQGIRAVDGWEYFLHGWSQIMARVFGFDLTPELF
                     TAMRDEAERWRT"
     gene            7815..8531
                     /gene="rlmB"
                     /locus_tag="VLE22_09475"
     CDS             7815..8531
                     /gene="rlmB"
                     /locus_tag="VLE22_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HSB14678.1"
                     /translation="MQLLGIHPVLEALRAKRPVERVLIAKGAAGSRLQEIIDLCREQG
                     VPSRFEPRQVLDRMAAGAVHQGVIALAAAHRYASFDQVIERAKMVVLLDGVEDPHNLG
                     AVIRTAHAAGVDALVIPERRAVGITEAVGRAAAGALEYMTIARTGNINRAIETLKSRG
                     FWIYGLDERGEHAYDAVEFTSPAALILGGEGKGLHRQVKEHCDFLVRIPMVGRISSLN
                     VSVAAGVVLFEWRRRLAAKG"
     gene            8574..10247
                     /gene="ggt"
                     /locus_tag="VLE22_09480"
     CDS             8574..10247
                     /gene="ggt"
                     /locus_tag="VLE22_09480"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSB14679.1"
                     /translation="MRESLALGALLVAVPWGFHARAAEPVRARHAMVVTREAHATDAG
                     VAVLRAGGNAVDAAVAVGFALAVTLPAAGNIGGGGFMLLRFTDGRTTFVDFRERAPKK
                     ASRDMYLGPDGNPTKDSEAGHLAAGVPGTVRGLELAHRKYGKKPWAGLLTPAVSLARD
                     GFSVSYGLARSLRASRLLARFPESRRIFQRDGAYYEAGERFRQPELAGTLDRIARSGA
                     ADFYEGETARRLAAGMAANGALITLEDLRQYSAVEREPLRGRYRGYEILTAPPPSSGG
                     TGILQMMGVLEKSGYEKHGAGSAASIHFVAETMRRFFAARNAYLADPDYVRVPVAGLL
                     DKRYVAKLRESIDPERATPSSEVSALDPAGYESSETTHYSIVDAQGNAASVTYTINGG
                     YGCGVTAPGLGFLLNNEMDDFTAKPGSPNAAGLVQGEANAIQPGKRPLSSMTPTIVLR
                     DGRLFLVAGSPGSGRIISTVLEVLLNVIDFGMNVRDAVDRPRFHHQWKPDTLFMEPGF
                     SPDTLALLRGRGHTVETVSGIGEAAAILAEGGWLQGAADSRSEGKASGY"
     gene            10253..11083
                     /locus_tag="VLE22_09485"
     CDS             10253..11083
                     /locus_tag="VLE22_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB14680.1"
                     /translation="MDHYLSTHLIVNHRLTTVWLDRIWLAGIPGVEIFCAKQHLDYRN
                     RAQINEIAHWFHDSELILHSLHSPMYTDDVWGRSGPHSVITITETSKPRRVEMLDEIK
                     RALEIAETIPFRYFIQHIGVAQEEYDDRKIEAAFTSLEELSLFAGHRGVQILLENIPN
                     GFSSAERLTYFLGETHLDLGFCFDTGHANMNEGVAAAFELMKSRIRSTHIHDNDGLSD
                     KHLFPTVTPGGTIDWKRTMELLRTNPSQYPLLLELKETAEIDHSLDAAKRVFENLENL
                     "
     gene            11084..12406
                     /gene="asnS"
                     /locus_tag="VLE22_09490"
     CDS             11084..12406
                     /gene="asnS"
                     /locus_tag="VLE22_09490"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HSB14681.1"
                     /translation="MNSAATSSRIAVAQAGDHVGEIVTVSGWLYNLRKSGKIVFPILR
                     DGTGVMQCVAVKSQLPEALFETIKDLTQESSIVVTGKVRAEPRAPGGYEMDVEAVEVV
                     QRVPDADPYPITPKEHGIDFLMDHRHLWLRSRRQHAVLRVRHEVIKAIRDYFDSNGFT
                     LIDTPIFTPAACEGTTTLFEVDYFDGEKVYLTQSGQLYNEATAMAFGKVYTFGPTFRA
                     EKSKTRRHLTEFWMVEPEMAYATLEDVKRVAEELVVFVVGRVLANRRSELETLERDIS
                     KLESVKAPFPRITYDEAVASLQRKGSEIQWGGDFGGTDETLLGEDYDRPVMVDRYPTA
                     IKAFYMQPDPERPEVALGVDVLAPEGYGEIIGGGQRIHDLSLLLQRIEEHGLPKEAFD
                     WYIDLRKYGSVPHGGFGMGVERCVAWICGLEHVRETIAFPRMLYRTRP"
     gene            12432..13346
                     /gene="rocF"
                     /locus_tag="VLE22_09495"
     CDS             12432..13346
                     /gene="rocF"
                     /locus_tag="VLE22_09495"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HSB14682.1"
                     /translation="MRTSHIAIIGAPLDLGAGRRGVDMGPSAMRVANLNARLASLGYE
                     VEDLGNIAVEQPESLPAGPERSRYLPHITQTCARLARLVEKAAGQGRAPLVLGGDHSV
                     AVGTVSGMSRYFRKRKQALGLIWIDAHADMNTPDTSPSGNVHGMPLSCCIGMGPRELT
                     HIGGYAPKVDPRNVALVGVREVDILEKPHVRESGVRAFTMRDIDERGLRAIMVDAIQI
                     ASAGTEAFHLSLDMDSVDPQEAPGVGTPVRGGITYREAHLAMELICDSGLMASIEVVE
                     VNPVIDEANRTANLAVELVMSAMGKRIL"
     gene            13362..14339
                     /locus_tag="VLE22_09500"
     CDS             13362..14339
                     /locus_tag="VLE22_09500"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HSB14683.1"
                     /translation="MRLRVAIIYGGRSGEHEVSLRSAQSIMAAMDRGTYEVIEYLIGK
                     DGHWKPGPILPEPGANAGIDVVFPVLHGTFGEDGTVQGLLELADLPYVGAGVLGSSVS
                     MDKDVMKRVCRERGLPVVEYVTLASPHLDPEAVREKLPFPVFVKPSNLGSSVGISKAR
                     DRKELEAALRMAAEYDRKVIVERAIDGRELECSVLGNSEPVASLPCEILPSREFYDYE
                     DKYLTDGAQFRIPAELNPAQTAEVQRLAVECYRAVECEGMARVDFFVEHSTGRIYINE
                     INTIPGFTSISMFPRMWEHSGLAYPRLIDRLIELALERHEARKSIRYTR"
     gene            14342..15799
                     /locus_tag="VLE22_09505"
     CDS             14342..15799
                     /locus_tag="VLE22_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HSB14684.1"
                     /translation="MLPLLPFLFLLLSAAPENDLESEARKFSEFYSIVESQAADPLVP
                     EKAFYDGAIPGMLRRLDPHSVFLNPEQFDQLKRMQESRSKGFGTVVSVVPGRVIILQT
                     LAGTPSAKAGLAPGDEIIAINGVQMNRLGVEQIIGLLGASRQRPVNMDVRRPGNVRTL
                     QFVMTPEEMQSPSVERAFFLKPDVGYVRVSSFDTETGKQLREAIERLGGAGLKGLVLD
                     LRNNPGGLLPAALETASLFLKPGQRLLAVRGRAIEPGEEKVPETSKPYEFPLAVLVNE
                     KSASGSEIVAGSLQDHDRAAIVGEPSFGKGLVESVYPLSEATALALTTAFYYTPSGRS
                     IQKPLRDGGQLDASTSRRPAQEFRTDSGRVVKGGGGIQPDLVVWQEQLSRLQMVLEAS
                     SSITSFAIEYTRKHAPITESFDVTPQVLDDFQAFLSPRNIRPGVEEWLRYRDWISNRL
                     KTEIFNQAFGVARGDEVEAQRDPVIQAALKTLGFG"
     gene            complement(15825..16478)
                     /locus_tag="VLE22_09510"
     CDS             complement(15825..16478)
                     /locus_tag="VLE22_09510"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSB14685.1"
                     /translation="MVETILDFLRTLTNPERLIALLTTALTGWLGYALLFVVVFSETG
                     LLVGFFLPGDSLMFTVGVVAGAGKLDIVLVNLVLMAAAIIGDGVGYALGRRTGPHIFN
                     RPNSRFFHQEHLRRTKAFYEKHGGKTIIYARFIPIIRTFAPFVAGVAEMGYGRFLSFN
                     IFGGIGWVFALTMLGYALGNVPVVRQHFEKAILLIIFISLLPILFEMYKAWRKKAPR"
     gene            complement(16508..18439)
                     /locus_tag="VLE22_09515"
     CDS             complement(16508..18439)
                     /locus_tag="VLE22_09515"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSB14686.1"
                     /translation="MFDLFRSRAKAVRYLLGAVLMLVAISMVVTLIPGFGTNVSSNDQ
                     IVAEVGKESITVLEVQQSIQGQLRGRNLPAEMAAAFVPQYIDQLVTENALAYQAERMG
                     YQVSKDDLGRTIESIFPQLFENGKFVGSQVYAAYLAQQNLTIPQFENNLRKQLLLNKL
                     RSLVLEGTIVPLDEVEREYRRRNEKVKIEYAAVTPEKYRTEVKVAPETIQSYFNANRS
                     QFTVPEKRSFQMLIIDQDKVGESVSIPESDLLQAYQAAQDQYRLPERVQVRHILLKTT
                     DVPKEEIPKIRARAEELLKQVRAGADFAELARKNSQDPGSAAKGGDLGWVLRGQTVKA
                     FEETAFSLKPKEISNLITTEYGFHILQVMAKETARLRPFAEVRSELATERKRQLVYDR
                     MQTLSDQARAALVKSPLSAAQIASDLGLQLVTVEKTGAGDPVPEVGVSQELQDALSSL
                     PKGDVTPVVQVQPTKLAVAVVTDVFPARPAELAEVEARIRDTLIQQELAKLVDERSRE
                     LVEKAKSMNGDLKRAAQAVGAEWKAPLEFARTGAAEGIGSASYLEQAFAGNEGDVFGP
                     VTVGDRRFVCKIVSKIPADMTGFAAERDNLQQELKSRIARQRNDLFEEGLKNRLIAEG
                     KIKLHRATISRIISNYAGR"
     gene            complement(18617..20041)
                     /locus_tag="VLE22_09520"
     CDS             complement(18617..20041)
                     /locus_tag="VLE22_09520"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSB14687.1"
                     /translation="MKDLLVMKFGGTSMGSAERIRVAAGICAAERRKRPVVAVVSAMS
                     KITDLLLDTLRRAEAGDQVDMESNLSRLQERHVETCRELLPPEKQEAVLAGIHSLISD
                     FQRIANGVLMLGERPPRSVDEAVAIGERLSAMLMASYLESTGVRSVAVNGADIIVTDA
                     VFGNASPLMGPTREKARRDLLPWIEGGVLAVVTGFNGSAADGRPTTLGRGGSDFSASI
                     LAAVLDASELWIWTDVDGILTADPRLVPDAAVLGEVTYSEAAELAYNGAKVLHPRTLA
                     PLIEKQIPVWSKNSFALDKPGTRIVSKTDSPGGPRAVTSMANVALISIESESSALSTN
                     QLLARAMDAMARTSVEVLVLTNSSYRQNFCFLVRKDELPVALSALESELSLELAHGYV
                     RPIEVDQNVGLLAIVGEGMRGTPGIAGRVFTAISRENINIIAIAQGSSELTIAVVVHR
                     DGLEKAVRAVHAECHMAERAHRAE"
     gene            complement(20038..20727)
                     /gene="cmk"
                     /locus_tag="VLE22_09525"
     CDS             complement(20038..20727)
                     /gene="cmk"
                     /locus_tag="VLE22_09525"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HSB14688.1"
                     /translation="MSKRIVVAIDGPSGAGKSTIARRLASRLGFLYIDTGAMYRAVGL
                     WALRLRVNLSDMHRLEQLAREARIELGPGDSTVFLNGEDISEAIRTQEIADAASRASA
                     VPGVRRAMVEKQREMASRANVVMEGRDIGTVVFPGAPVKVYLDATPEVRAGRRVKELR
                     EKGLEAHVDEIAGQIRERDRRDSTRADSPLMQANDAVYVDSSGLDIGEVEEAILRIVR
                     DRTSNGKDHAS"
     gene            20768..21133
                     /locus_tag="VLE22_09530"
     CDS             20768..21133
                     /locus_tag="VLE22_09530"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HSB14689.1"
                     /translation="MHPQEFDRLVEKALLAIPSRFRRRLKNVAFVVEAEPPVPNLLGL
                     YQGRPLTRRSVGDGFTMPDRITIFQGPHERLARSPVHLEKLLEDTVWHEVAHYFGMDE
                     RTVRHAERRRASLRRRREP"
     gene            complement(21149..22141)
                     /locus_tag="VLE22_09535"
     CDS             complement(21149..22141)
                     /locus_tag="VLE22_09535"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HSB14690.1"
                     /translation="MKPLCAVLTVCSLLALATAPVPAETHRVQAAGFHNTFSGAHAAL
                     AKIKPGDRVITQTIDARGSDSQNKQVGKGPNPQTGPFFVEGAERGDVLVVRIEKIEPN
                     RATAWSDNTLAPYSVDPAFLRSEALRERKTLTWNIDTAKGVAWVESPDIRPRRIEVPL
                     RPMLGCVATAPDNKEAIATSTPDNFGGNMDYNGMVAGVTLMLPVYEPGALLFVGDGHA
                     RQGDGEVIGNALETSMNVEFSVDLIKKKAIRWPRLENKEEIVVLGSARPLLQALQHAT
                     TEMQRWLMEDYGFDERGASLLMGHALEYDIANIVDPHFTVAAKMPKRFLPGSTK"
     gene            complement(22202..23755)
                     /locus_tag="VLE22_09540"
     CDS             complement(22202..23755)
                     /locus_tag="VLE22_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14691.1"
                     /translation="MPRKAPPHGESGFALLFVFFMAAAIAISFYIQLPVAVFENQRTR
                     EQALLDRGEQYRRGIQLYFRKFKRYPAELKALEKTNNIRFIRRLYKDPLTGSDEWRLI
                     HISPAGVLTDSLVQKPGEKEDESKSQNTFITEGPSVGAAVESQTSTSAPWMRRRGGTA
                     GGPGIPGAGASGDVAAPENTPQPGAEVQPAGPEEESGDQPDEPQSKQQAQSQEGFAPV
                     APQAGQATGQPGVLYPQQTVEGQNPAIPGQQPQTEQPGAPPFPFLPQPGGQQQTGGFQ
                     AGTPGQRPFQQQVIPRPAVLPVQRIPGAPVPYSQPIQNQPGAQSGSPPPFIGGPPTPS
                     PSDSGSPPPFIGGPPAPPSTVGGLVGGGPAGGPVFGGQPVGGAQSTSQPGQNPAADLI
                     RKLLTTPNPRGLAGTQQQGLGGQQGLGGQQALGRQGFGGGIAGVASKKESDSIKVYNE
                     RQKYQEWEFIYDYRQDRMNMGLTGAGGTQGGTGQRNLQGTSPLSPGMGPGTQPGPLTP
                     GPRGRGRLQ"
     gene            complement(23755..24462)
                     /locus_tag="VLE22_09545"
     CDS             complement(23755..24462)
                     /locus_tag="VLE22_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14692.1"
                     /translation="MKVGAEPKKVAILGGLLVVAVYFLYSNLFSGGGTAPVPASQAQS
                     PAAPPPAPVAESGRPVPRAEPPRANRRSLQDFRPSLRPRRAPTERPDLSSIDPTLRVD
                     LISRLEKVALSGGPRSLFEFSAAPLPKTPEPKIIPKLMVGPVPPPANPSKPGSEVAAK
                     QPATPIPLRFFGYTVQARLGNKRAIFLDGDEIHVASEGDVVKKRYKVVRIGVNSVVVE
                     DIEQKNEQTLKLEAEAS"
     gene            complement(24459..25016)
                     /gene="pilO"
                     /locus_tag="VLE22_09550"
     CDS             complement(24459..25016)
                     /gene="pilO"
                     /locus_tag="VLE22_09550"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HSB14693.1"
                     /translation="MAKDPRVTVRVVLGLLLIANLVAAAAVFKPWGGSPEDLQRQLAQ
                     LSSDAAKRETSLAHLRKLVTTVEKTRSDANKFLEQYFLARRSAYSTILGELAELAEKS
                     GVKMKEHSFVTEPIEGSDTLGMMVITGNYEGAYADIVKFVNQIDRSPRFLTIDTLQAT
                     PQQGQGVLNVNMKLNVFVREEPLAR"
     gene            complement(25084..25650)
                     /locus_tag="VLE22_09555"
     CDS             complement(25084..25650)
                     /locus_tag="VLE22_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14694.1"
                     /translation="MRISLNLSSEPFRRDRLILVASTFVGVLLAALLVLLVSLAVIER
                     GQAAETRTAIDTIEKRLRAMTAEETSLEAALVRPENAEVLDLVVFINTLLYRKGISWT
                     RIFADLADVVPHDVRLISVRPQVNAKNEILLDMVVGSQSQTPVVDMLKRMESSPMFGD
                     TAVHSWLPPAQTEPLYRYRVSVNYAQKL"
     gene            complement(25658..26554)
                     /locus_tag="VLE22_09560"
     CDS             complement(25658..26554)
                     /locus_tag="VLE22_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14695.1"
                     /translation="MSFLQSITGLLKDPPPAFAFELSEAGMASAQNGKPPVLYFQEFE
                     KDVISVSPVRDNILRPDAILGLVKAVAPGGDGRKRRTAALILPDYSVRVSVLDFDSFP
                     SDAKEQASLVRFRLKRSVPFDVESAAVSFHPQPNAKNSKKVDVIAAVAPLEVVARYEA
                     PFRAANLHPGWVTTSILAAVQLVPPTGLKVLAKRTGRILSIAVVDSGAVRLVRSIELA
                     DVSAHEIVGHLFPTFAYIEDELSARPDMLLLCGFEPNDDRLHDLLQGELGVPVAALIS
                     RLGTTGEFNSGLLGYMESLEGA"
     gene            complement(26566..28188)
                     /locus_tag="VLE22_09565"
     CDS             complement(26566..28188)
                     /locus_tag="VLE22_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSB14696.1"
                     /translation="MATDRVKPLDPEQETAQARSLAARYRCEFVDLTKARIDHDLFRS
                     IPVDLMFRYNFVPMHAENGSLDIALADPRNLNLIDELAILLNRKLRIKVATLSQISEL
                     LKKTEQSQRVLEEVTEGFALDVVGDEENQDETLSIDRLTAADSDIAPVIKLVDTTIFN
                     ALERRASDIHIETRDQEVAIKYRIDGVLHYAMPPISKDWHSTIISRIKVMSELDIAER
                     RVPQDGRFRVRYKNRLIDFRVSIMPTIYGEDAVLRVLDKESMSEKFAKLSLDVVGFSD
                     SDLTKFRRYIKEPYGMVLVTGPTGSGKTTTLYAALSEIKNDEDKIITVEDPVEYQIKG
                     ITQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDQETAQIAINAALTGHLVFTTVH
                     ANNVLDVLGRFLNMGVEPYNFVSALNCILAQRLVRVICGFCKRPAEHPDSYLVESGLD
                     LAEWREVTFYEGAGCFECGGTGFRGRTAIHELLDLSEHIRQMILDKRPSAEIKRAARE
                     EGMTFLRESALEKARLGVTTLREINKVTFIEA"
     gene            complement(28258..29469)
                     /locus_tag="VLE22_09570"
     CDS             complement(28258..29469)
                     /locus_tag="VLE22_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSB14697.1"
                     /translation="MAEYLLKYADTRGEIHQQVAEADSEHDLRQRLSDQGFLVYSIRP
                     RSGLMPIGRGTLPRRKKLNVEKFLIFNQQFVTLVRAGLPILKALELLADRLTDPKLGR
                     YIQAVRDDVRNGAMLSDAFRRQGVFPPIYVTSILAGEKSGSLAEVLDRYIAYQKLALA
                     IRKKLLVSLMYPAVLIVLVIALMVFLVTYVVPNFAQLYSSMSANLPTVTQLLIAVGTT
                     ARNYVLAFAVGLVALGFAFRFWSRRESAQEALDRVKLRTPLVGGIWIKYQVSQFARVL
                     STLLLGGIPLVQALDTASESLGSRLLHKALEKAKRMVREGQPLSSSVAATGIFPALSI
                     DMIEVGESTGALPAMLNSVSEFFEDDVNIRMAAALSLIEPAIMIFMGIFVAFVLVALY
                     LPIFSLADTLK"
     gene            complement(29472..30146)
                     /locus_tag="VLE22_09575"
     CDS             complement(29472..30146)
                     /locus_tag="VLE22_09575"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HSB14698.1"
                     /translation="MAPETVKHDVTRWAVWGCLLLSPGLLPGQELSALLKSVELRYNR
                     ARTIQVLFEQSYSVQGRRRIVESGELSLRRPGRMRWQYTSPEGKLFVSDGKKVFLYSP
                     ATNRVETMGLKESDDMRAPLAFLLGKLDFWRDFQKFVSKPDGSDLRITAEPKSNKLPY
                     TKVEFVVTPQYEIRYLNITGQDHSVMEFRFSREKLNPPLPDRMFRFQPPPGAELVDVA
                     ADGEVR"
     gene            30254..30496
                     /locus_tag="VLE22_09580"
     CDS             30254..30496
                     /locus_tag="VLE22_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14699.1"
                     /translation="MAWKSPFQQGAQVEHPKFGFGSVLSSNEEHIVIKFDDHGEKKFV
                     TPIVLPNLKKSDRQPPAEKRASRSRKPKVVAPAVAG"
     gene            30554..31222
                     /gene="purQ"
                     /locus_tag="VLE22_09585"
     CDS             30554..31222
                     /gene="purQ"
                     /locus_tag="VLE22_09585"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HSB14700.1"
                     /translation="MRFGVVVFPGSNCDHDAWYATSRNLGQESEFIWHDAHSLGNVDA
                     VILPGGFSYGDYLRCGAIAKFSPVMKAVRKFAAGGGLVLGICNGFQILVECGLLPGAL
                     IRNRGLKFICRQVELSTVTTNSPFTCQAAIGQVLRVPIAHGEGCYYADGRTLDELEAE
                     DRVVFRYVDNPNGSLRDIAGILSRERNVMGMMPHPERASDPLMGCTDGLAVFRSMVAA
                     MAVK"
     gene            31224..33440
                     /gene="purL"
                     /locus_tag="VLE22_09590"
     CDS             31224..33440
                     /gene="purL"
                     /locus_tag="VLE22_09590"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HSB14701.1"
                     /translation="MSAITPEVVASHQLSESEYDKIVSLLGREPTFTELGIFSVMWSE
                     HCSYKSSRLHLKKLPTRSKLVLQGPGENAGIIDIGDGYAIAFKIESHNHPSFIEPFQG
                     AATGVGGILRDIFTMGARPIAVMDSLRFGPLDDPEVGARNRRILEGVVAGIAHYGNCF
                     GVPTVSGECVFEESYNGNPLVNVFALGVFRTDEIFYAKASGIGNPVIYVGAKTGRDGI
                     HGASMASAEFTEESKQKRPNVQVGDPFLEKLLLEACLEAMQTGAIVGIQDMGAAGLTC
                     STCEMGSRGGTGIEIDLKYVPQRETRMTPYEIMLSESQERMLLVAEKGREEEVFAVFR
                     KWGLDAVTIGEVTGDGLLRVRDRGVVVAEIPNSALADEAPMYDRPHMAPLRKAPMEGP
                     PFASGDLQADLATLLAAGDICSKRWIFEQYDFMVRTNTVQGPGGDAAIVRIKETDSSI
                     AMSLDGSGRYCSLSPREGAKLAVAECCRNLSTVGALPVAATNCLNFGNPERPEIMAQL
                     VEAIEGMGEACRFFDTPITGGNVSLYNETLGEAIDPTPVIGIVGLLKTGAPLTIDFKQ
                     PGRTVVLLGGLGNCDDARFGGTQYAKVVLNEMWGMPPALDMAYEKRIHAAMRAIAEAG
                     LAESAHDLSDGGLAVAAAESSFGPVAVGAELVLDSGLRPEFLLFHEAPSRILLSTSRP
                     EEVQRIAREHSVEAPLVGATVKGRLSITNRGRTLVDCEVGELRSLWEGALERALHA"
     gene            33444..34859
                     /gene="purF"
                     /locus_tag="VLE22_09595"
     CDS             33444..34859
                     /gene="purF"
                     /locus_tag="VLE22_09595"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HSB14702.1"
                     /translation="MQDQFHDECGVFAIYGHPEAANLTYLGLYALQHRGQESAGIATS
                     DGTEINCYKAMGHVADIFTPAVLAAMPGDAAIGHTRYSTAGDTVLLNAQPFSVACNKG
                     RIAVAHNGNITNAADLRRELEREGSIFQASSDTEVILHMVARSRERTLAGALREALLQ
                     LEGAFSLVFLAEDRVIVARDPHGFRPMAMGQMELSHGKICYVFASETCAFDLLGAVYL
                     NDVEPGEMVIVGPEGLTRERYAPEQPKAQCVFEHVYFSRPDSIVFGRSVSESREMLGR
                     LLAREHAVDADVVVPVPDSGVAAAIGYSAESGIPYRQALIRNHYVGRTFIEPSQAIRD
                     FGVKLKLNPVRRLLEGQRVVLVDDSIVRGTTSRKIVRMVRSAGAREVHLRISCPPTIS
                     PCFYGVDTPTKNELIASNNTVEEIRRFVEADSLGYLSLSSLREAVHDDKHEYCYACYT
                     GDYPTDLVNIEDLIAAKSRRG"
     gene            34863..36041
                     /locus_tag="VLE22_09600"
     CDS             34863..36041
                     /locus_tag="VLE22_09600"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSB14703.1"
                     /translation="MTPKVASSALAVPHSRIRELAEVAMSMDGVLKLYFGESNLPTPE
                     YIKQAAVQALSDGYTFYTENAGLPSLRRALAGYYHRVQGVELDPEREIVITASGVQAL
                     CVGIRCVLDPGDEAIVLTPAWPNGSASAALSNAVARQVPHPLCGERYMIDFAALEAAV
                     TPRTRMLLYTSPSNPLGWVATGDDQQRLLGFCRRHGLWLMADEVYDRLYYEGGAPGDP
                     VPSILRMTTREDAVTVVQSFSKSYCMTGWRVGWLVARADVASRATQLNEFIISHAPSF
                     AQKAAETALAEGEEELARMVGRLKDNRDFCWATLREMPGITVPKPEGAFYLFPRIDGL
                     TDSFEFCRRLLLEEKVGLAPGVAFGAGGEGSVRICYAAERSILEEAMERLGRFLRRGV
                     "
     gene            complement(36131..36601)
                     /locus_tag="VLE22_09605"
     CDS             complement(36131..36601)
                     /locus_tag="VLE22_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HSB14704.1"
                     /translation="MLLLLLVVTLACQQQPAPPPDTRAADESAIRVAETEWSNRAFAK
                     DLEGILSYYAEDASMLTPNAPIATGREAIRKAWSEMLALPSLALSWQTVKVEVARSGD
                     LAYTYGSYEMSVNDPKGKPVKDLGKYATVWKKQANGSWKAVVDIFNSDQPPPKS"
     gene            complement(36749..37669)
                     /locus_tag="VLE22_09610"
     CDS             complement(36749..37669)
                     /locus_tag="VLE22_09610"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB14705.1"
                     /translation="MNRRAFLGVTGSLPLAWADSVVSPAAIIDTHTHFYDPSRPEGVP
                     WPSKGETVLYRTVLPDEFVRLTKRMGVTGTIVVEASPWLEDNQWVLDVAEKHPVILGL
                     VGHLEPGKPAFQNHLERFGKNPLFRGIRLAAKVIADGLSHTPFLKDLKGIAAAGLEID
                     AVGGPAMLDDLLRLADGVPDLRIVIDHLPFDPPAEASDREAFHRALREVGKRPLVYMK
                     VSSVLRRKGSKAPTELSAYRPALDELWEVFGPGRLIYGSNWPVSDLVAPYDAVLRVVR
                     EYFTAKGQDAVDSYFWKNALEAYRPPRFRA"
     gene            complement(37888..38964)
                     /locus_tag="VLE22_09615"
     CDS             complement(37888..38964)
                     /locus_tag="VLE22_09615"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB14706.1"
                     /translation="MRYVPICFLCAASVFAQTMSIEEYDPKSTLVVREHPLTRARYPF
                     IDVHSHQNSQMPADSLDRLVKDMDGLNMRVMVNLSGGSGDRLQSGVANMKGRYKDRFV
                     IFANLNFAGIDDPDWGKRAAAQLEQDVKNGAQGLKIFKNLGLTLADSQGRRIPVDDPR
                     FDPVWEACGRLGIPVLIHTGEPWSFFQPHDRFNERWLELKEIPSRARPADRFPSWEQI
                     MGEQHKMFAKHPKTIFINAHLGWLGGNLAELGRLMDTLPNMYTEIGAVLAELGRQPRF
                     AREWFIRHQDRVMFGKDSWNATEYHTYFRVLETADEYFDYYRKRHAFWKMYGLELPDE
                     VLKKLYYKNALRVIPGINPAGFPK"
     gene            complement(38997..39728)
                     /locus_tag="VLE22_09620"
     CDS             complement(38997..39728)
                     /locus_tag="VLE22_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSB14707.1"
                     /translation="MSTLRALLLTDPLIVLATAVMGTLSFFASLYDRSGNLPHRIARC
                     WSRMLLLLSGARVHAEGLDKITPGGSYVIASNHLSLMDTPLLMAYLPVQFRFLAKKGL
                     FRVPFIGGHLKRAGHVPIPRHDPRGSLKAMSEAARMVQAQGVSALVFPEGGRSATGGL
                     NDFKEGAAYIAIKAGVPVVPVSIQGTREILPMNSVVVRPGRVRLQIGDAIPTAGLTLR
                     DREWLTTEIRDRILELLGETVRNAS"
     gene            complement(39735..41003)
                     /locus_tag="VLE22_09625"
     CDS             complement(39735..41003)
                     /locus_tag="VLE22_09625"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HSB14708.1"
                     /translation="MNYPESVRFLYALGNEVKTAKLGLDRIAALLARLGDPHRACRFV
                     HVAGTNGKGSVCAMIESALRTAGQRTGLFTSPHLVEPTERIRIDGEPVDPGLFARAFN
                     QVHREAEALLAEGSLDFHPTYFETVTAMAFVLFRDLGVETAVLEVGLGGRLDATNVVT
                     PTLCVITPVDFDHEAFLGRSIEAIAAEKAGILKSGVPAVIAVQRPGILEFLEERAAVA
                     GTSVRPAGDWEVRDLDFDRRGSRFLAVGAARIPVECPLAGEHQVMNALTAVAALYEMG
                     VPGDRITEGIRRAAWPGRLECVSEQPEIILDGAHNPSGARALAGHIRRFYHGRRVRLI
                     YGCMRDKAVTEMAGILFPLADEVILTAADSPRSIRPQALRELVDHPCIQVTANLREAL
                     RFMDGAASQDAFFITGTLFLVGEARALLVK"
     gene            41145..41315
                     /locus_tag="VLE22_09630"
     CDS             41145..41315
                     /locus_tag="VLE22_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14709.1"
                     /translation="MRATSRIESLSYGTIYMTGCIVALFLSFGQHSPLQRVLVHTVCS
                     WGYVIYWVLNYQ"
     gene            41427..41903
                     /locus_tag="VLE22_09635"
     CDS             41427..41903
                     /locus_tag="VLE22_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HSB14710.1"
                     /translation="MPMLQKLLAYLDEKQVQYTHHAHPTAYTAREVASVEHVPAQTIA
                     KTVVYFSENGYGVVVLPGDSMVDLHALRESLGVARLRLATESELGDLFPDCELGAMPP
                     FGNLRDLPVYVDGRLPAEETITFNAGTHRDVIQMKYADFERLVRPAVLTISKHAAA"
     gene            41990..42538
                     /locus_tag="VLE22_09640"
     CDS             41990..42538
                     /locus_tag="VLE22_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB14711.1"
                     /translation="MIFREVEDPDLIAKARRGKVEAYNLLISRWEKRVFNYLLRLVGD
                     REDALDLSQEVFLKAYQNLGKLQDPTRFAPWLFRIAHNEAFSHLRRRRPDEPFEVFPV
                     ESGAATRQPPIELTLAVEAALRRLSEEQREAVLLKIYQGFKFEEIAEILSCPVSTVKS
                     RVYSALDVLKQILAPIDTRGVE"
     gene            42535..43119
                     /locus_tag="VLE22_09645"
     CDS             42535..43119
                     /locus_tag="VLE22_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSB14712.1"
                     /translation="MSCTQVDLKGFFLNELVPDARQAVEEHLRVCPNCREELERLSVT
                     RAALTSLREEETPRRIAFVSDKVLEPSIWRRFWDSAPRLGFASAAMLSVALLVNGFIR
                     PQPAAPAPMDSAAVQAKIEAVVAGRVAEAVQKAVADSEARSARRTADLLAATEQRLDR
                     QHRADLLTVEDNFRVLQKRVNIYYRASNDLGSAQ"
     gene            43116..43667
                     /locus_tag="VLE22_09650"
     CDS             43116..43667
                     /locus_tag="VLE22_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14713.1"
                     /translation="MRRALCIASVLVAAAAAGDPPKPRVTRAELAAVETSFDQRIARL
                     SVADDPMEVLGSTRGVYLEGYGAVFTTEVNLVITPPITPFRMKITKEEVARVHQRKLE
                     RLPTLKRAMRAMLVSSAASLDDVPTNEQVVVGVTLFFLNWEDRTGLPSQIVMQAPRRP
                     LLEYNLSTSTSEALEAAIREQVF"
     gene            43671..45308
                     /gene="gspE"
                     /locus_tag="VLE22_09655"
     CDS             43671..45308
                     /gene="gspE"
                     /locus_tag="VLE22_09655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HSB14714.1"
                     /translation="MRLGEMLIARRLIEPEDLERALEIQQERGEKLGKILVDLGFVAM
                     RDVLSALAEQLAVPLVTIDSPPPVSPEIEGLSPRFMRQCRFLPVAMRDSTLTVAMADP
                     LDFETLSAVGGFTGLKVEPVLAAEQEILDGIDKFFGEAEKGAAGFDEDSAQAAEDLEH
                     LRDMASEAPVIRLVNAMIAQAVEKRSSDIHIEPFEKEFRVRYRVDGVLYNQEPPPREL
                     KAAIISRVKLMAKLNIAERRLPQDGRIKIKTLGREVDLRVSTLPTLYGESVVMRLLDR
                     SAGDFYDLRRLGFDEHMLSRMEHYTSLPHGILLVTGPTGSGKSTTLYSALKRINQPDK
                     KIITIEDPVEYQMDGINQIHVNPQIGLTFASGIRHIVRQDPDVIMIGEIRDRETADIA
                     IRAALTGHFVYSTLHTNDAPSAITRLSDMGVENYLLCSSLVAVLAQRLVRVICEHCKA
                     PDSVRTAPDGRPLRCWRGKGCERCFGTGFTGRVGIFELMEMNEEIRSLIMKDADAAQI
                     TVAARHHGMRNLREDGWLKVESGLTTWDEVMRVTQEF"
     gene            45308..46516
                     /locus_tag="VLE22_09660"
     CDS             45308..46516
                     /locus_tag="VLE22_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSB14715.1"
                     /translation="MASFHFRAVAADGKVRTGTLSGETEKTVAMELRRQGLAPVYVGL
                     QPKKAFQLRLPSFVRGRRKDVLFFTQELSTLLNAGVPLDRALAITAELGERPNFRFIV
                     SDVLRVLKGGKSLADSLATHPEYFSELYVNMVRAGEASGALSVVFERLAEFENSRDDL
                     RNYIISSMIYPALLSLVGLGSILVLVNFVAPRFAAVFQESRIPMPLPTRMMLEASRLL
                     RAYGWIAVLAVAGAGAVFYSYIRTAVGRLWWDTLRLRVPLLGGALRRAETARFARAMG
                     TLVANGVGLVQAIGIAGAILNNRRIAGSLDAVAQGVKRGEGIAAPLSRSGQFPPLAGH
                     LLTVGEETGRLDQMFSRMAEIYENETRAAIRRFTALFEPMVILVMGVIVGALILSMLL
                     AITSINEVAV"
     gene            46520..46957
                     /gene="gspG"
                     /locus_tag="VLE22_09665"
     CDS             46520..46957
                     /gene="gspG"
                     /locus_tag="VLE22_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HSB14716.1"
                     /translation="MSDRKKRKQAGVTLIEMLVVVTIIALFAALVGRNIFRQTDKARI
                     TAARAQIEIFMGALGQYKLDTGGLPTTDQGLLALWTRPADAPPSWDGPYLAKEVPPDP
                     WGNAYVYRFPGEHGDMPDIVSYGADGQPGGEGFNADIISWKSQ"
     gene            46954..47520
                     /locus_tag="VLE22_09670"
     CDS             46954..47520
                     /locus_tag="VLE22_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSB14717.1"
                     /translation="MKPSGDLLNARLRGRRAASTVDVQAPESTGREINRSQSGVTLIE
                     MLIVVAIISLMVGISYPSISSGVDALRLSSASDSIASFLNGALNRAERKQVVVEVTIS
                     KLENALIMRSEEPGFERRLDVPQGVRITGVLPANLVDPDAPRHFLMYPGGTLPRFGVE
                     LANQRGGRRIVRVDPITGNSQVERPEAQ"
     gene            47517..47945
                     /locus_tag="VLE22_09675"
     CDS             47517..47945
                     /locus_tag="VLE22_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSB14718.1"
                     /translation="MRERGFTLLEMLVATVIMGVAVVGLLSAISASLHNASRLTDYDR
                     AALAAKRKMDELLLDSRLPRLSVLEGRFDAEPGSGFEGGWRARLAPYEMPPATGPSTL
                     VLERLELEVWWMSGGRRRTIALEGFRSSTAAGLAPAEGQP"
     gene            47942..48676
                     /locus_tag="VLE22_09680"
     CDS             47942..48676
                     /locus_tag="VLE22_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14719.1"
                     /translation="MRRTHTAGVTLMELLIAVSLLALLATGILAAMRIGFNALGKTNR
                     RLMDNRRVAGAQRILEQQIAGFIPVVADCLPDPERPPVPAPFFQGEPQSMRFVSAYSL
                     GEAWRGLPQTVEFTVVPGKDGRGVMLVVNEHLYTGPRGPGLFCLGSEFDPELGRPAPR
                     FRPIEVGPRSFVLADKLAFCRFAYRELMPPPVLERWIPRWTAPQWPTGVRIEMAPLEH
                     DPARLQPLTLTAPIRVNRLPGEAYAD"
     gene            48819..49793
                     /locus_tag="VLE22_09685"
     CDS             48819..49793
                     /locus_tag="VLE22_09685"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14720.1"
                     /translation="MPVSRRGGALLVVMWLAAALSAIAFTLAGTVRTETERTSTVLGG
                     VRAYYLATGAIDRAALYMLWGRQFTMPDGSPRYYAPWMNALQFSFESGEARVEIIPDT
                     SKMNINTAPPEDLVQLLLALGADPERANLVSQAIVDWRAPRVNGEPGPFDQYYLSLTP
                     SFPSRHASFQEVEELLLVRGVTPELFYGGFVRSPEGRLIRTGGLRDCVSVTGSSREFD
                     VNTAHPAVLAASGLDPGTVAAIVETRSRMPFRSQEDLNALGIPPGARRLRIGGNRIFT
                     LRATARLRVSDKEFSDLRRSVAATVKLEASPDGRPYRVLRWYDNVWVQ"
     gene            49790..51100
                     /locus_tag="VLE22_09690"
     CDS             49790..51100
                     /locus_tag="VLE22_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14721.1"
                     /translation="MTAHDVRRILAFGTGVGLEIGDRDLRVVVVRIRPSSVSVLGSAT
                     ISDFRGRPAAEWGAEYFEFVRGAGAGHVAAFALLPRREVIVRHVNLPGVADRDLGAAI
                     EYQIDALHPYGEGEAHYSWARLSDKSTVLVGITRRTVIAQYHEVLGEAGIKLAALTFS
                     AATLYSAARLFGAPPADGFLALAGQDGEMELYGESASRPVFSAVMGGPPEKAAPLAAA
                     ELRLAPDAALLSLSQILPAPKLAPADFDVECFALPYAAALAGASPRLALPINLLPPDL
                     RSTSSRAMFVPTAALATVLAGLLVALAAQARLQERRHLEAVQAEIAKFERRASRVAAA
                     ETETGKLRARIHLLDAFRQRTASDLDAIKEITALLAPPAWLSSLELTRDSLSLAGEIE
                     QAAPLLKTLDGSPRFRGSEFTVPILRTAKVEVFRIRASREEVAP"
     gene            51097..51684
                     /gene="gspM"
                     /locus_tag="VLE22_09695"
     CDS             51097..51684
                     /gene="gspM"
                     /locus_tag="VLE22_09695"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HSB14722.1"
                     /translation="MTLQKRDKRALVILGVAAAALIVWNYLLTRESATPVVSAAGSLP
                     AAEKRLVRLRQTAALLPAREETLKAVRDQLALREKGLIQAETGPQAQAQLLQILRRLT
                     AAQSIEVRTVEMGQVRTLGDDYGEVSVPVTFDCRIEQLLNLLADLTSQSEAVAASEVR
                     IGVANEKEKMITVRLTVSGVVPRRLVPEKKGVARF"
     gene            51681..52412
                     /locus_tag="VLE22_09700"
     CDS             51681..52412
                     /locus_tag="VLE22_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14723.1"
                     /translation="MTLRKKLLLLNLVLLALIAAVSWRLRQEWLAARAREQEVLRQAL
                     KPAPAPPMSPLPQPKSVTAAAYDDIAQKVLFSKDRNPVVTIEVAPPKPLPPFPVSHGV
                     MNLGDGPMILLSEQPGKPHRAYTPGDKVGEYKLVAANNEEIVLEFDGRQVKKRLDELI
                     DRSAKEEPSPAPGAGRRSNAAQPAAQHPSPAAGSQSGPGAVMSGEVRACLPGDTAPAG
                     TVNNGLRKVITESPFGKVCRWEPVR"
     gene            52443..52892
                     /locus_tag="VLE22_09705"
     CDS             52443..52892
                     /locus_tag="VLE22_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14724.1"
                     /translation="MKRILLLLLSLALFAFGAEEKKKAPRPKPGQKAQEIALPAGAQE
                     IAPYTYRYKDDQGKTWIYRRTPFGLAKFEEKLDDKADEIAPENMRAFEEGDSIRFERP
                     TPFGTARWVRKKSELNDVEKAAWERELKRSVKTADRKQEQSQAKSQQ"
     gene            52901..>54971
                     /locus_tag="VLE22_09710"
     CDS             52901..>54971
                     /locus_tag="VLE22_09710"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HSB14725.1"
                     /translation="MRFWISTGTMWVVLAAVALAQQKQPEPIQVVPPPGFGPRVAPPA
                     RTGTPKSKRTKPQREQSKPAAESAKPEAQPIKPETQPAKAEAKPEAGQPGAAPAQPAP
                     QEVAPVKPGPVTLSPTPSGRFVLNLENASLIQVIEILTRRLKLNYILDPRVNVKGGGI
                     TLNTFGELKEVDIRALLDTILRMNGAAMVQVGELHRIVPLTEITRMPLSPQVDPKSIP
                     EDERVVLNLIFLKYATVTEMAKILEKFLGEGAVMVTYEPANLLLVLDNNRNMRRTMEL
                     VALFDSGALASQRVRLFEVKNNRPSDLVKDLETVLQGASLTGKLGGVRFLALDRINTI
                     IAVAPNPGVFEEVEKWLGKLDVAVKTTAGATDNYVYRVKYGRAPMLATAIMHLYLGIP
                     FSGFGYGGGMYGGGMYGGGRRGGYGGYGGFDGGGYGGYGGGYGGYGGGYGGYGGGYGM
                     PGGYGGYGRGGYGGYGRGYPGSGYGGVSPYMMSSGGIVPPPAATGTAGTVGATAGAQQ
                     ALPSGAATSPDMTGSYLGSWDYGLPEGIPRIVPNPLDNSLLIQATPQQYEQILKLLRD
                     LDVPPRQVLIEAKIYEVSLTGAFAGGVKAYLQRKGATGTGAATFGRILQGTTSGAGLT
                     LTAGMLVGESRELLGILTAQETQGKTRLISAPSVIATDSIPASINVGQEVPTLASQAV
                     TGAQQAGT"
BASE COUNT        10193 a        16763 c        17732 g        10283 t
ORIGIN      
        1 agtcacccgg ctcttcagaa aactcgacga gacggcgcgc cgcggttttg tcatcagcga
       61 tctggtgcga agccgtgccg ctcacgcggg cgtctggctc gccacgcatt tgcttagccg
      121 caatcgcctc atccgccacg atggcccgct gagcgtccgc cgcgcgtata ccgtcggcga
      181 actgaacgaa ctggcgcgcg ccgccggcct cagcgatctg cgtttcatcc accggccgct
      241 gttccgcctc gtaggagtgc tgtgcaaatg agtatggcgg gcggaggtgg tacggatctc
      301 gacccgggcc aactttggga cgcgatcgtg gctggcgcgg ggcccgccgg cagcacgaca
      361 gccgccctgc tcgcggaacg cggcttccgc gtactgctgg tcgatcgcgc aaccttccca
      421 cgcgacaaga tgtgcggcga gtacctcgga gcaggctgtg ttgagttgct cgccggcttc
      481 ggcgtcgaac tgcgtgatac gcaccccatt cgcgccatgc gcgtcatggc gccggacggc
      541 acggtcttca ccgcaccgta tccggagggg cgcaacggcc tcgccatccg ccgttcccaa
      601 ctcgatgcgc tgctgctcga aaacgcgagg tggaagggag cgctgtgtct ggaaggtttc
      661 cgggttgaga ggctgcttct caaggacgga aaagtagacg gcatcgaggg ccggctccac
      721 ggcggggagg aaaaacagtt ccgtgcgcgg atcgtgatcg gcgcggaggg acggaattcg
      781 gtagtcgccc ggcatttggg cctctttcgc tggaaccgca gtcatcgccg gacagctctc
      841 gggatccatt acgaaggagt cctgccggtc agcggcttcg ccgaggtctt tttgggggcc
      901 ggattgtacg gtatcctgaa tccgctggga gcgggggagg cgaacctcag cctggttgtc
      961 gctcacgacg gctcactctc caaggaccgc gccggtgcgt actttgcgga ggcgctgggg
     1021 cgtctgcccg cactccagga acgcctggcg caggcccgac cgctcgaaga agtgaaggtt
     1081 ctgggaccaa tggcgcacca tgcggcgaga gcttccgcgg acggggcgct gcttgccggg
     1141 gacgccgcgg gattctatga cccgttcacc ggcgagggcg tgtacatggc gctaatctcg
     1201 gcacggctcg cggccaacgt cgccggagag gcgttgcggc gacgcgattg ttcctcccgt
     1261 tttctgcgcg cctacgaccg cgaacgctcc gcggcgctgg gaggccggta ccggctagag
     1321 gcggcaatcc agcggattat ccgtcatccc cgcgctgccg acctcgtatc ccggaggctc
     1381 cgcgaaagag aagacgccgc ttcctgtctc atggaggtgc tcggaggcct cgcctggccg
     1441 cgcgccctat tgtctggagg gtttctgtcg gcgttcctat gattcctctt ctgttcctcc
     1501 tcctctggcc ggccgccgct cccgacactg aggccgaaaa aatccagccg cgtcgcgaga
     1561 cgatcgtcgt gacggggagc ttcgaacctg ttccgctcga ggaagcggac cggtctgtag
     1621 acaagctgga cgtcgaaggc cacccgttgc tgcttcgcaa catcgccgag gtgctccgcc
     1681 tgatcccgtc tgtggatctg cgggagcgcg cgcctggagg cgttcaggca gacgtctcaa
     1741 tccgcggagg gactttcggc cagacattgg tgctgcttga cggactgcga atgaacgaca
     1801 tccagtcggc acatcacaac atggacttgc ccgtgccgct ggagtcggcg gccgcggtgg
     1861 aggtgctgcg aggttcgggc tcggctctgt atggctcaga cgcagtcggt ggggtggtga
     1921 acatcgtaac caggaagccg gaggcagggg aggtacgtct gcgggccggg gttgggaatt
     1981 tcggggtgaa cgcccagagc atctcgatcg ccggggttaa gggtcgcgtt tcccagcacc
     2041 ttgcgctgtc gcgagacttc tcgagcgggt ttgcgccgaa cagggactac cggagtctgt
     2101 cgcttttctc acggttgtat ctcgaaagtg cgtggggctc gtcgacggtg ctgctcgcgc
     2161 acggcgaccg tccgttcggc gctcaaaatt tctacggtaa ctttccttcc tgggaacgga
     2221 cgaagacctg gttcgccgca ttgcggcagg acctcggcgc acgcaccgag gcgtcgttcg
     2281 cgttccgccg gcacacggat ttgttcgtgc tgtaccgcga ccgcccgcag gttttcacaa
     2341 accggcacgc tgttgagagc tatcagggcg cgctgcgacg gcgcgaggag gctgggcgca
     2401 acacgcagat tcacttcggc gcggaggttc tcggcgatac gatcacgagc aataatctcg
     2461 gccaccacca acgggcgcgg ggagccgcct acgccgcgct ggacatgcgc gcagtgcgaa
     2521 ggttttcctt ctcgctggcg gcgcgggagg agatctacgg gccgctcaga agccagttca
     2581 gcccgaccgc tgcggcaggc gcgtggctat cgcagcggat caagctgcga gcgtcggcga
     2641 gtcgggcgtt ccggctgcct tcgtacacgg acctctacta tcacgacccg gcgaacctcg
     2701 gatcgccgga tctgcgtccc gaaaccgcgt ggagctacga cgccggcgtg gactggaacg
     2761 cgggcggcaa attgtcgggc gaggtaaccg tgtttcaccg gcgggagagg gacggcatcg
     2821 actacgtgcg cagcacgccg gcggagcttt ggcgcgcaac caacatccag cggctgaact
     2881 tcacgggagc cgaggtagcg gtgatcgcag gccttggcgg atcacaccgc ctcgatttcc
     2941 gatacaccgg cctgcggggt gcgcagaagg ccctcgcggg gcttgaatcg cgttacgttt
     3001 tccaataccc cgtgcatgcc ggagtggcct cgtggatggg gagtctgccg ggaggtttag
     3061 ccgggcgagt gcaactcggc gcaacgaggc gttatgccag cgatccgtac gcggtgcttg
     3121 acgtcaccgt cgcgaggacg ctggggcgag tgcgcccctt cctgcagttc gccaacctcg
     3181 ccgacgccgg ttacgaggag attcgcggcg ttccgatgcc ggggcgggcg gtgatgggcg
     3241 gcgtggcggt gatcctgtcc ggtggaggga agtaggctaa actgtaggag aaggtaagga
     3301 atttttgatc cggtttctcc tcgcggcaat cctcccgctt gcatattgtg gcgttggtgt
     3361 cgcggccgac cagggccaac tagacgcgag tccgactctc ttcagcgtgc tggcggcgat
     3421 caatgcggcg ggctacgatg cggacatcga ctccgcggcg aaccatccgt tccgggagat
     3481 cgtgcgcaag gaactggctt cgcggaacat cccctgtctc gaggatctga aacgcttctt
     3541 ttccgaacac cgtcagaagg atgagactgc ggaattgagc cagtatgttt cgtttgcact
     3601 ggtggtggac gaagcgccgg gctttaacta ccggctgcac cagaacgaac tgccaccgga
     3661 cgtggttccg ctcgaaggct tcgagaaact gatggcgcgg ttccataagg aggccggaat
     3721 cgatgaactg tggcgccggg cgcagccgga gttcgaagcg gtgatcgcgc gctaccacga
     3781 accggtgact cgcgcggtta ccgaggccaa cgcgtacctg cgcagcgcaa cgagcggcta
     3841 tctcggccgc cggttccaga tctatgtcga cctgctcggc gcgccgaacc agatccagag
     3901 ccgcagctat gccgatgagt acttcatcgt gctgacgcct tcgcccgagc cgcaggtcga
     3961 ggacgtgcgg cacgcgtatc tgcactatct gctggacccg ctggcgtcga agttcgccga
     4021 ggaaatactc aagaagaagg atctgggcga ctacgcccag ggcgctccgg ctctggagga
     4081 gtactacaag tcggactttc tgctgctgac caccgagtct ctgatcaagg cggtcgaggc
     4141 aaggctggcg ccgcgtggaa acaggcaggc gttggtggac caggccttca aggaaggttt
     4201 cattctcaca ccgcacttcg cagaacagtt gccggttttc gagtcgcagg agcaggcgat
     4261 gcgactgtac tttccggagc tggcgaagtc catcgacttg aaaaaggaag agcgccgatt
     4321 ggagagtgtg gagtttctcg cggagcggcc cgtacgcaag gcaaaggtgg tgccggcgga
     4381 aagaaagatc gaactgacgg gtgcgaacaa gacgctggaa gacgcggagg aggcttaccg
     4441 cggccgggaa ctcgctgccg cgaaggagcg ttaccttcgt gtgttacagg agacgagcga
     4501 gaagccgctg cacgcgaagg cgtactatgg gctggcgagg atcgccgcgc tcgaaaagga
     4561 tccggaactt tccgaacggc tgtttctgaa gacactcgaa ctggctccgg agccgcaggt
     4621 gaaggcgtgg acactcgttt acctaggccg gcttgcggac gcagcgggcg agagggaaca
     4681 ggccggaaag cattatcagg ccgccatcgc ggtggcagga gcatccgcag cggcgcgcaa
     4741 ggcggccgaa caagggttgc aggaatcgtt cagaaaggat aagtaagtaa aggccggtca
     4801 tcttggattt tggatttgat gaaggccgta ctttaggagt catacatgag aaacggagca
     4861 gtgttgagat ttgtcctcgg cgtggcgtgc gccaccggaa tgctgttggg acaaggggcg
     4921 aagcagttga cggtgaagtc gaagggcgag gcggaagcgg tcatggcgct tcagactgca
     4981 ttgcagtccc agagcccgga cgggatcatc accgcgtccg acaatctgct gaacaagtac
     5041 gccgacacgg atttcaaggt catcgcactc tattattctg ccgaagcgta cgggatgaag
     5101 aacgacttcg acaagatgat cgtctatgca gagcggacct tgcaggcgga ccccaagcac
     5161 taccaggcga tgcttctgct tgccggtgcc atcgcgcaac gcacgcgcga gcacgatctg
     5221 gacaaggaag agaagctgaa gtcggttgag aagtacgtgc agcaggccga cgaggcggtg
     5281 aagacggcgg taaagccgaa tccgaacctc accgacgagc agtgggaggg actcaaaaaa
     5341 ggcttcgaag cgcagggact ggaagcgctc ggcttggcct cactcgcccg cgcgcagaac
     5401 gacaaggcca tcgagcaatt caagaaagcg gtagagatcg acccgacgcc caacccgagc
     5461 acaatgttgc ggctggcagt cgcttacacc aaggcgggaa aacccgatga gtcgatcgcg
     5521 gttctggaca aggttctagc cacgcccaac ctgcatccac agatcaagca gatcgcccag
     5581 gcggaaaagg cgagggctac acaggccaag agcggaggag cggcgccggc caacaacgca
     5641 ccgccgcaga tcgaagtcaa gaaaccgtaa ccgggccgca gatccaatgc gcgcggaacc
     5701 ggacgtcctt gaggaggcga ttggccacgg cttccacgac cgtgacatcc tgtcccgcgc
     5761 gctgactcac aagtcgttcg tccatgaaaa gttcgccgcc ggtgaagcca cgctcgggga
     5821 taacgagcag atggagtttc tgggtgacgc catcctcgga ttcctggtca gcgaagccct
     5881 gatccggcag catcccggcc ttcctgaggg acaactctcg aaactgaaag cccacctcgt
     5941 cagcgcgtcc catctccacg aagtagcgca ggaactcagg ctcggggact acctgctgct
     6001 aggccgtgga gaggagttga gcggaggccg ggacaagaag gctctactgg caaacgcggt
     6061 tgaggcgctg atcgccgcga tctacctgga tggcggaatg gaggccaccc ggcaatttgt
     6121 gctcgggcac gtggccggtg gggtcgaagc gctttcgcaa ggtccggcac tgccgatcat
     6181 cgacttcaag agcgctctcc aggaactggc acaggcccgg cggctgccag cgccccgcta
     6241 cgcaatcatt aaggagcgtg gtcccgagca cgccaagacg ttcaccgtgg aggtgcgcat
     6301 cggccacgat ctgaccgggc aggcagaggc gctctcgaaa aaaagcgcag ggcagaaggc
     6361 cgcacaggtt ctgctcgaga gactcagcgg ggacgactct taagggcggc ctcagcgacg
     6421 ttttagcagc gcgagaatct cggcaagttc gtggcggatg ctttcgagag cgggcggggc
     6481 agcaaagagc gtcgcctgcg aagactcctg cgcgggcttt tggacggcgc ggcgcggccg
     6541 cgattcgagg tatttcctgg ctccttcgat cgtatagcgg cgctcgtaaa gcaggcgttt
     6601 cacctcgagg agaagttcga cgtctttgcg ccggtaaagc cgccggccgg tcccggattt
     6661 cttcggcgaa agcgcgggga aactcttttc ccagaagcgc aggacatggg gcggcactcc
     6721 gaccaactgg ctgacttcgc cgatacggaa gtagagccgg tccggaattt gtggtgtgtt
     6781 gtcgtccaga ggcaggtcgg ccatgttcgc ccgcgcaaca accgtcatgg tacgggatgg
     6841 gtggggctgc gtcaacctct cgaagtaagg ccttcagaaa tttgggctta ggcggcaggg
     6901 ttggcggcgc tccgggcact acgttatcct tcggatcggc atgcggcgga tgtatttcat
     6961 aggcgtgacg acggcggcgt cctcgattca cgcactcttt ccgaagtgga cggagcttgc
     7021 ggggatcgag ggttcggaac tcgtgggcct cgatatcgaa cccggggcgc cgccgcaagc
     7081 gtatcgggag gcgatgcgca ggcttcggga cgatacagcg gcgtggggcg cgctggtcac
     7141 cacgcacaaa actgctgtgt atgctcatgc ccgggacctg ttcgacggct ttgacgccga
     7201 tgccgaggct ctgcgcgagg tcagttgcat cgtgcggcgc gaagggcgta tgacgggcgt
     7261 cgccatcgac acgctgacct ccggcctggc actgcggcag gtgacgggca acgggccatt
     7321 tcagggacag gctctgatca tgggggcggg aggagcggcc gtggcactgg cggtccacct
     7381 gtcccggcgg caccgtccgt cgcgcgtgat tcttacggac gtatccgccg gcaggatcgt
     7441 cgaagcgagc gaattggcgc cggcggagtg catgctggtg aatgttcccg aggaccacga
     7501 ccgcattctc ggggcgatgg cggaaggcgt cctggtagtg aacgctaccg gaatgggaaa
     7561 ggaccggccg ggttgtccga ttaccgtgca agcccggttt ccggccgggt cggtcgcttg
     7621 ggacttcaac tatcgcggcg atctcctgtt tctcgaaaat gcacgggcac aggggattcg
     7681 agccgtcgac ggctgggagt atttcctgca cggatggagc cagatcatgg cgcgggtttt
     7741 cggattcgac ctgactccgg agcttttcac ggccatgcgc gacgaagcgg aacggtggag
     7801 gacttgaacg ggtgatgcaa cttctcggga ttcatccggt gctggaagcg ctgcgggcga
     7861 agcggccggt ggagcgggtg ctgattgcga aaggcgcggc cgggtcgcgc ctccaggaga
     7921 tcattgacct ttgccgcgag cagggagtgc cgtcgcggtt cgaaccgcgg caggtgctgg
     7981 accggatggc ggcgggcgcc gtgcaccagg gcgtgatcgc actggccgcg gcgcaccgct
     8041 atgcttcctt tgaccaggtg atcgaacggg cgaagatggt tgtcctgctt gatggcgtcg
     8101 aggatcccca caatcttggg gcggtgatcc ggacggcgca cgctgccggg gttgatgcgc
     8161 tggtgatccc cgagcggcgg gcagtcggga tcacggaggc ggtcggacgg gcggctgcgg
     8221 gagcgctgga gtacatgacc atcgcacgga cgggcaacat caaccgcgcg atcgagactt
     8281 tgaagtcccg agggttttgg atctacgggc tcgacgagcg gggcgagcac gcatacgatg
     8341 ccgtggaatt cacgtcaccg gcggcgctga tcctgggggg tgaaggcaag gggttgcacc
     8401 gtcaggtgaa ggaacactgc gatttcctcg tccgtatccc gatggtgggg cggatctcat
     8461 ccctcaacgt ctcggtagct gcgggagtcg tgctgttcga gtggcggcgg agactcgctg
     8521 cgaagggcta gaagcgggaa gccgccgttc acggtcggct acaatcgagt tttatgcgcg
     8581 aaagtctggc gcttggcgcg ctgctggtcg cagtgccgtg ggggtttcac gctcgggcgg
     8641 cggaacccgt ccgcgcgcgc cacgccatgg tggtgacgcg cgaggcgcac gccaccgacg
     8701 cgggcgtggc ggttctgcgc gcgggcggta acgcggtgga tgccgctgtg gccgtcggat
     8761 tcgcgctggc ggtcacgctt ccggcggcgg gcaacatcgg cggcggtggg ttcatgctgc
     8821 tccggttcac cgacggacgg accacctttg tcgatttccg cgagcgcgca ccgaagaagg
     8881 cgtcgcgcga catgtatctg gggccggatg ggaatcccac gaaagacagc gaagccggcc
     8941 accttgcggc gggggtacca ggaacggttc gcgggctcga gcttgcgcat cggaaatacg
     9001 gcaagaagcc gtgggcgggt ctgctcacac cagcggtgag tctggcccga gacggttttt
     9061 ccgtatccta cgggctggcg cgatcgcttc gggcaagccg cctgttggca cgatttccgg
     9121 aatcccgccg gatcttccag cgcgatggcg catactacga agccggggag cggtttcggc
     9181 agccggagtt ggcgggcacg ctcgaccgca tcgcgcgaag cggcgccgcc gacttctacg
     9241 agggcgagac ggcgaggaga ctcgccgccg gcatggccgc caacggcgcg ctgatcacgc
     9301 tcgaggatct aagacagtac tcagccgtcg agcgcgaacc gctacggggc cgctatcgtg
     9361 ggtatgagat tctgacggcg ccgccgccga gttcgggcgg cacaggcatc ctccagatga
     9421 tgggtgtcct ggaaaagtca ggttacgaga agcacggagc gggttcggcc gcctcgatcc
     9481 acttcgtcgc cgagacgatg cggcgctttt tcgcagcgag gaacgcatat cttgccgatc
     9541 ccgattacgt ccgggtgccg gtcgcgggac tgctcgacaa gcgctacgtc gcgaagctgc
     9601 gggagtcgat cgatccggag cgcgccacgc cgagctcgga agtgagcgcg ctcgaccctg
     9661 ccggctacga aagctcggaa acaacgcact actcgatcgt cgatgcgcag gggaacgccg
     9721 ccagtgtcac gtatacgatc aacggcggct acggatgcgg agtgacggca ccgggattgg
     9781 gattcttgct caataacgag atggacgatt tcaccgccaa gcccggctcg ccgaacgccg
     9841 ccggcctggt acagggcgaa gcgaacgcga tccaaccggg caagcgtccg ctctcgtcga
     9901 tgacgccgac gattgtgctg cgcgacgggc gactgttcct ggtggccggc tcgccaggca
     9961 gcggccggat catcagcacg gttctcgagg tgctcctgaa cgttatcgac ttcggcatga
    10021 acgttcgcga cgcagtcgac cgtcctcgct ttcaccacca gtggaagccg gacacgttgt
    10081 tcatggagcc gggattctcg cccgatacgc tggcgcttct ccgtggacgg ggccacaccg
    10141 tggaaaccgt ctcggggatc ggcgaagcgg ctgcgattct ggccgagggc ggatggctcc
    10201 agggcgccgc ggattcgcgc tcggaaggca aggcaagcgg ttactgacac gcatggatca
    10261 ctatctatcg actcacctga ttgtgaacca ccggcttact accgtgtggc tcgacaggat
    10321 ctggctcgcg ggcattccag gagtggagat tttttgcgcc aagcagcacc tggactaccg
    10381 aaaccgggct cagatcaacg aaatcgcaca ttggttccac gactcggaac tgattctgca
    10441 ctcgctgcac agcccgatgt acaccgacga cgtttggggg cggtccgggc cgcattcggt
    10501 gatcaccatc acggagactt ccaagccccg gagggtcgag atgctcgacg aaatcaagcg
    10561 cgcactggag atcgccgaga ccatcccgtt ccgctacttc atccagcaca tcggggtggc
    10621 gcaggaagag tacgacgatc ggaagatcga agccgcgttc acgtcgctcg aggaactgag
    10681 tctcttcgct ggacatcgcg gcgtacagat acttctagaa aacatcccga acggattctc
    10741 gagcgccgaa cgcctgacct attttctggg cgagacgcat ctcgacttgg ggttctgctt
    10801 cgatacgggc catgcgaaca tgaatgaagg ggttgcggcg gccttcgaac tgatgaagag
    10861 ccgcatccgg tccacgcata ttcatgacaa tgacgggctg agcgacaagc atctcttccc
    10921 gaccgtgact ccggggggca cgatcgactg gaaaagaacc atggaattgc tgcgcacgaa
    10981 ccccagccag tatccgctgc tgctggagtt aaaggaaacc gccgagatcg atcactcact
    11041 cgatgcagcg aagcgcgttt tcgaaaacct ggagaacctc taaatgaatt ctgccgcaac
    11101 ttccagccgg atcgcggtcg cgcaggccgg cgaccacgtg ggcgagatcg tcaccgtatc
    11161 gggctggctg tataacctcc ggaagtcggg aaagatcgtg tttccaatcc tgcgggacgg
    11221 caccggcgtc atgcagtgtg tagccgtcaa aagccagctt ccggaggcgc tgttcgagac
    11281 gatcaaggat ttgacgcaag agtcgtcgat cgtggttact ggcaaggttc gggccgagcc
    11341 gcgcgcccct ggtggatacg agatggacgt cgaggcggtg gaggtcgtcc agcgtgttcc
    11401 cgacgcggat ccctatccga tcacaccgaa ggaacacggt attgacttcc tgatggacca
    11461 ccggcacctg tggctacgta gccgccggca gcacgccgtc cttcgcgtcc gccacgaggt
    11521 gatcaaggca attcgcgatt atttcgactc gaatggcttc acgctgatcg acacgccgat
    11581 cttcactccg gcggcgtgcg agggaacgac aaccctgttc gaggtggact acttcgacgg
    11641 cgagaaggtt tatctgacac agtccgggca gctctataac gaggccacgg cgatggcttt
    11701 cgggaaggtt tacacctttg ggccgacctt ccgggccgag aaatcgaaga cacggcggca
    11761 tctgactgag ttctggatgg ttgagccgga gatggcgtac gcgacgctcg aagatgtcaa
    11821 gcgcgtggct gaggaactag tggtgttcgt ggtcggccgc gtgcttgcga accggcggag
    11881 cgaactggag accctcgagc gggacatttc aaaactcgag tcggtcaagg cgccgtttcc
    11941 gcgaatcact tacgacgagg ccgtggcaag cctgcaaagg aagggcagcg agattcaatg
    12001 gggcggtgat ttcggaggaa ccgatgagac gctgcttggc gaggattacg accggccggt
    12061 catggtagac cgttatccga ccgccatcaa ggcgttctac atgcaacccg atccggagcg
    12121 gccggaagtg gcgcttgggg tggatgtgct ggcccccgaa ggctacggtg aaatcatcgg
    12181 cggcggccaa cgaatccacg acctgagcct gctgctgcaa cgcatcgagg aacacggact
    12241 gccgaaggaa gcgttcgact ggtacatcga cctgcgaaag tacggaagtg ttccccacgg
    12301 tggtttcggg atgggcgtgg agcgctgcgt ggcgtggatc tgcggactgg aacatgttcg
    12361 cgagacgatc gcctttccgc ggatgctcta ccgcacgcgg ccgtgaccac tactcgcggc
    12421 ggggcaagaa catgcggacg tctcacatag cgatcatcgg cgcgccactc gatctcgggg
    12481 cagggcggcg cggcgtggac atgggcccgt cggcgatgcg ggtggcgaac ctaaatgccc
    12541 gcctggcttc actcggctac gaggtcgagg acttgggcaa catcgccgtc gaacaacccg
    12601 agagcctgcc agcgggtccc gaacgttcgc ggtatctgcc gcacatcacg cagacctgcg
    12661 cgcggctggc gcggctggtg gagaaggcag ccgggcaggg cagggcgccg ctggttctcg
    12721 gcggcgatca ctcggtggca gtcggcacgg tctcgggaat gtcccggtat ttccggaagc
    12781 gaaagcaggc gctcgggctc atctggatcg acgcgcacgc cgatatgaac acgcccgaca
    12841 cgagtccgag cggcaacgtc cacggcatgc cgctgtcgtg ctgcatcggc atggggccgc
    12901 gcgaactgac gcacatcgga gggtacgcgc cgaaggtgga cccgcggaac gtcgcgctgg
    12961 tcggcgtgcg cgaggtggac atcctggaaa aaccgcacgt acgggagtcg ggagtgcgcg
    13021 cattcaccat gcgcgacatc gacgagcgcg gcttgcgggc gatcatggtg gacgccatcc
    13081 agattgcctc ggccgggaca gaagctttcc acctgtcgct ggacatggac agcgtggacc
    13141 cgcaggaggc gcccggcgtg ggcaccccgg tgcggggcgg catcacttat cgcgaggcgc
    13201 acctggcgat ggaacttatc tgcgattccg ggctgatggc ctccattgag gttgtcgagg
    13261 tgaaccccgt catcgacgag gcgaaccgga ccgccaacct ggccgtggag ctggtgatgt
    13321 cggcgatggg gaaaagaatt ctgtagacga aaaggacgcg agtgagactc agggttgcga
    13381 tcatctatgg cgggagaagt ggcgagcacg aggtctccct gcggtcggcg cagtcgatca
    13441 tggcggcgat ggaccgaggg acgtacgaag tgatcgagta cctcataggt aaggatggcc
    13501 actggaaacc ggggccgatt ctgccggagc ccggcgcgaa tgccggcatc gatgtcgtat
    13561 ttcctgtgtt gcacgggacc ttcggcgagg atggaacggt gcaaggcctg cttgaactcg
    13621 cggacctgcc gtacgttggc gcgggcgtgc ttggctcgtc ggtgtccatg gacaaggacg
    13681 tgatgaaacg agtctgccgc gagcgcgggc tcccggttgt ggagtacgta acgctggcgt
    13741 caccccatct cgatccggaa gccgtccgcg aaaagctccc gtttccggtg ttcgtgaaac
    13801 cgtccaatct cggttcctcg gtcggcatct ccaaggcgcg cgatcgcaag gaacttgagg
    13861 cggctctgcg catggctgct gagtacgacc ggaaggtcat tgtcgagcgg gcgatcgacg
    13921 gccgtgaact ggaatgttcg gtgctcggta acagcgaacc ggtggcgtcg ctgccctgcg
    13981 agattctgcc atcgcgcgag ttctacgatt acgaagacaa gtacctcacc gacggcgcgc
    14041 agttccgtat tccggcggaa ctgaacccgg cgcagaccgc ggaggtgcag cggctggcag
    14101 tggaatgcta ccgggcggtg gagtgcgagg gcatggcgcg ggtggacttc tttgtcgagc
    14161 actccacggg caggatctac atcaacgaaa tcaatacgat tccgggcttc acttccatca
    14221 gcatgtttcc ccggatgtgg gagcactcgg gcctcgcgta tccgcgactg atcgaccgcc
    14281 tgatcgaact ggcgctggaa cgtcatgagg ccaggaagtc gatccggtac acccgatagc
    14341 gatgctaccc ctgcttccct ttttgttcct cttgctgtcc gcagcgccag agaacgatct
    14401 cgagagcgag gccaggaaat tctccgaatt ctactcgatc gtcgagagcc aggcagccga
    14461 tccgctcgtg ccggagaagg ccttctacga cggtgcgatc ccggggatgt tgcggcggct
    14521 tgacccgcat tcggtctttc ttaacccgga gcagttcgac cagctcaaac gaatgcagga
    14581 atcgaggagc aagggttttg ggaccgtggt ttccgtcgtg ccggggcgag tgatcattct
    14641 tcaaacgttg gcgggaacgc cgtcggcgaa agcgggtctc gcgccaggcg acgaaatcat
    14701 cgcgatcaat ggagtccaaa tgaaccgtct cggtgtcgag cagatcatcg gcctgctcgg
    14761 tgcatcgcgt cagcggccgg tgaatatgga cgtgcggcgg cccggcaacg tgcggacgct
    14821 ccagttcgtg atgacgccgg aagagatgca gtcgccgagc gtcgagcggg cgttttttct
    14881 aaaaccggac gtgggctatg tccgcgtctc cagtttcgac acggaaacag gcaagcagct
    14941 ccgagaggcg atcgagagac tgggtggcgc cggcctgaag gggctggtgc tcgacctgcg
    15001 caacaacccg ggagggctcc tgcccgcggc attggaaacg gcatcgctgt ttctgaaacc
    15061 tgggcagaga ctcctggccg tccgaggtag ggccatcgaa ccaggcgagg agaaggtgcc
    15121 cgaaacatcg aagccttacg aattcccgct cgcggttttg gtgaacgaga agagcgcgag
    15181 cggatcggag attgtcgcag gcagcctcca ggaccacgac cgggcggcga ttgtgggcga
    15241 gccgagtttc ggtaagggtc tggtggagag cgtctacccg ctatccgaag caacggcgct
    15301 ggctctgacg acggcattct attacacgcc gagcggacgc tcgatccaga aaccgcttcg
    15361 ggacggtggc caattggacg cgtctaccag ccggcggccg gctcaggagt tccggacgga
    15421 ttccggtcgt gtcgtcaagg gcggcggtgg gatacagccg gacctggtgg tgtggcagga
    15481 gcagctttcg aggcttcaga tggtgcttga ggcgagcagt tcgattacgt cgttcgccat
    15541 cgagtacaca cggaagcatg ctccgatcac cgagagtttc gatgtcacac cgcaagtgct
    15601 cgacgatttt caggcgttcc tatcaccgcg caacatccgt cctggcgtag aggagtggct
    15661 ccggtaccgt gactggatct ccaaccgtct gaaaaccgaa atcttcaatc aagctttcgg
    15721 cgtcgccaga ggtgatgagg tggaggcgca gcgggaccct gtgatccagg cggcgctgaa
    15781 gacgctgggc ttcgggtgac tgacggccgt ccggttcggg cggttcagcg cggggccttc
    15841 ttgcgccacg ccttatacat ttcgaagagg atcggcagca gggagatgaa gatgatcagc
    15901 aggatcgcct tttcgaaatg ttgccggacg actgggacgt tgccgagcgc atagcctagc
    15961 atggtcagag caaagaccca gccgattcca ccgaagatat tgaacgagag aaaccgtccg
    16021 taacccatct ccgcaacccc ggcgacaaac ggtgcgaagg tgcggatgat agggatgaaa
    16081 cgcgcgtaga tgatcgtctt ccccccgtgt ttttcataga aggccttggt tcgccgcaga
    16141 tgctcctggt ggaagaagcg ggagttcgga cgattgaaga tgtgggggcc ggtacgccgc
    16201 ccgagggcat agccgactcc gtcgccgatg atcgccgccg ccatcagtac gaggttgacg
    16261 agcacgatat cgagctttcc ggcaccggcg acgacgccga cggtgaacat cagcgaatca
    16321 cccggaagga agaacccgac cagcagaccc gtctcggaga aaaccaccac aaacagcagc
    16381 gcatagccga gccatccggt gagcgccgtg gtcagcaggg cgatcagccg ctccggattg
    16441 gtcagcgtgc gcaggaagtc caggattgtt tctaccatcc ggttgaccgt ctgatcggga
    16501 tcgcgctcta ccttccggcg taatttgaga tgatccggct aatggtcgca cggtggagtt
    16561 tgatcttgcc ctccgcgata agccggttct tgaggccttc ttcaaacaaa tcgttgcgct
    16621 ggcgggcgat tcgtgacttc aattcctgct gcaggttgtc ccgctccgcg gcgaacccgg
    16681 tcatgtcggc ggggatcttc gaaacgatct tgcagacgaa acggcggtcg cccacagtaa
    16741 ccggaccgaa tacgtcaccc tcatttcccg cgaaggcctg ttccaggtag gaggcagatc
    16801 cgataccctc ggccgcgccg gtgcgagcga attccagtgg cgccttccac tccgccccta
    16861 cggcctgtgc cgcgcgtttg aggtcgccat tcatcgactt cgctttttcg accagttcac
    16921 gactgcgctc gtcgacgagc ttcgcgagtt cctgctggat gagcgtgtcg cggattcggg
    16981 cttccacttc ggcaagctcc gcggggcggg ccggaaacac atccgtcacg acagcaaccg
    17041 caagcttggt tggctgaacc tgaacaaccg gcgtgacgtc acccttcggc agcgaactga
    17101 gagcatcctg aagttcctgg ctgaccccga cttcgggaac ggggtcaccc gcgccggttt
    17161 tctccacggt cacgagctgc agccccaaat cggaggcgat ctgggcggcg ctcagcgggc
    17221 tcttgaccag agccgcgcga gcctggtcgg agagggtctg catgcggtcg tagacgagtt
    17281 ggcgctttcg ctcggtggcc agctcgctac ggacttccgc aaacggccgc aaccgcgctg
    17341 tttccttcgc catcacctgc aggatatgaa agccgtactc ggtggtgatg aggttgctga
    17401 tctctttcgg cttcagcgaa aacgcagttt cctcgaatgc cttcaccgtt tggccgcgca
    17461 gcacccaccc gaggtcgccg cccttggctg ccgagcccgg atcctgggaa ttcttgcgcg
    17521 ccagttcagc gaaatctgcc ccggcacgga cctgcttgag gagttcttcc gcccgggcgc
    17581 ggatctttgg aatctcttct ttcggaacgt ccgtggtttt gagcaggatg tggcgaacct
    17641 gcactcgctc gggaagacgg tactgatcct gtgctgcctg atacgcctgc agcaggtcgc
    17701 tctccgggat cgagacggac tcgccgacct tgtcttgatc gatgatcaac atctggaagc
    17761 tgcgcttttc cggtactgtg aactgggagc ggttggcgtt gaagtagctc tggatggtct
    17821 ccggcgccac ctttacttcc gtgcgatact tctccggcgt gacggcggcg tattcgattt
    17881 tgaccttctc gttcctgcgg cgatactcgc gctcgacttc gtcgagcggc acgattgtac
    17941 cttccagaac caggctgcga agcttgttca acaacaactg cttccggagg ttgttttcga
    18001 actgggggat ggtcagattc tgctgggcga ggtaagccgc gtagacctgg ctgccgacga
    18061 atttcccgtt ctcaaacaac tgagggaaga tcgactcgat cgtgcggccg agatcgtcct
    18121 tgctaacctg gtagcccatg cgctccgcct ggtaagcaag ggcattctcg gtgaccaatt
    18181 ggtcgatata ctggggcacg aaagcggccg ccatttcggc gggaagattc cggccgcgta
    18241 gctggccttg gatggactgc tgcacctcaa gaacggtgat cgattccttc ccgacttcgg
    18301 ccacgatctg gtcattcgag ctgacgttgg taccgaagcc ggggatcaga gtgaccacca
    18361 tcgagatggc gaccagcatt agcaccgccc cgagcaaata acggacagcc ttcgcgcggc
    18421 tgcgaaacag gtcaaacatc gaagagaatc tcctgaaact tgtaacgcct ggccatcagt
    18481 agtccacgct gagcatccgg gcggcacata aaccagcgag cacggcgcat cgtgatctgc
    18541 attcgttctg gaggctgaag gctttgaatc ctatagtata gcggagcgcc ggagttcccg
    18601 gcggctcact ccgttattat tccgcccgat gcgcccgctc agccatgtgg cactcggcgt
    18661 gaacggcccg cacggccttc tccaagccgt cccggtgaac gaccaccgca atcgtaagct
    18721 cgctcgatcc ctgtgcaatg gcgatgatgt tgatattttc ccgggagatg gcggtgaaaa
    18781 cgcggccggc gattccggga gtgccgcgca tgccctcgcc gacaatcgcc aacagcccga
    18841 cgttctggtc cacctcgatg ggacggacat agccgtgcgc cagttcgagc gagagttccg
    18901 actcgagagc ggagagcgcc accgggagtt catctttgcg gaccaggaag cagaagttct
    18961 ggcggtagct ggagttggtg aggacgagga cctcgacgct ggtcctcgcc atggcgtcca
    19021 tggcgcgcgc cagtaactga ttcgtgctga gggcggagct ctcggattca atagatatca
    19081 gcgccacgtt ggccattgac gtgacggctc gcggcccgcc gggcgaatcg gtcttcgaga
    19141 cgattctcgt tccaggtttg tcgagggcga agctgttctt gctccaaact gggatctgct
    19201 tctcgatcag gggcgccaac gttcttggat gcagtacctt ggctccgtta taggcaagtt
    19261 ccgcggcttc gctgtacgtc acttcgccga gaaccgcggc atccggcaca aggcgggggt
    19321 cggcggtgag gatgccgtcc acgtcggtcc agatccacaa ctctgaggcg tccagcaccg
    19381 cagccagaat cgaggcggag aaatcggagc cgccgcggcc gagcgtggtt ggcctgccat
    19441 ccgcggccga gccgttgaac ccggtgacca ccgcaaggac tcctccctcg atccagggca
    19501 acaagtcgcg gcgggccttc tcgcgggtcg gccccatgag cggcgacgcg ttgccgaaaa
    19561 cagcatccgt cacgatgatg tcggcgccgt tgactgcgac cgagcggacc ccggtgcttt
    19621 ccaggtagga cgccatcagc atcgcggaaa gccgctcgcc gatagccacc gcttcgtcca
    19681 ccgagcgagg cggccgttcc cccagcatga gaacgccgtt cgcaatgcgc tggaaatcgg
    19741 aaatcaacga gtggatgcct gccagcaccg cctcctgctt ttctggcggg agcagttcgc
    19801 ggcaggtttc gacgtgccgc tcctgaaggc gcgagaggtt cgattccatg tcgacctgat
    19861 ctcccgcctc ggcgcggcgc agcgtatcga gcagcaggtc cgtgattttg gacatcgccg
    19921 aaaccaccgc caccaccggc cgtttccgcc tctcagcggc gcagatcccg gcggccacac
    19981 ggatgcgctc ggccgatccc atactcgtgc cgccgaattt catcaccaac aggtccttca
    20041 agatgcgtgg tcctttccgt tcgacgtgcg gtcccgcacg attcgcaaga ttgcctcctc
    20101 gacctcgccg atgtcgaggc cgctcgaatc gacgtagacg gcgtcgtttg cctgcatgag
    20161 aggcgagtcg gcacgtgtac tgtcgcgccg gtcgcgttct cgtatctgcc cggcgatttc
    20221 gtccacgtgc gcctcaagtc ctttttcgcg aagttccttc acgcgccgtc ccgctctgac
    20281 ctcaggggtc gcgtcaaggt agactttcac cggtgcaccg ggaaacacga ccgtgccgat
    20341 atcgcgtcct tccatgacga cgttcgcacg ggaggccatt tcgcgctgct tttcgaccat
    20401 cgctcgccgg acacccggga ccgcggaggc acgcgatgcc gcatcggcga tttcctgggt
    20461 ccggatggcc tcgctgatgt cttcgccgtt caggaatacc gtggagtcgc ccggcccgag
    20521 ttcgatccgg gcctcgcggg ccagttgctc cagccggtgc atgtccgaga gattaacgcg
    20581 cagccgtaga gcccagagtc caaccgcacg gtacattgct ccggtgtcga tgtaaaggaa
    20641 accgaggcgg ctggcaagac gccgcgcgat cgtgctcttg cccgcaccag acgggccgtc
    20701 aatggccacc acgattcgtt tgctcataag ccggcaccgc gccgggcttg cctacaatgg
    20761 ttcacagatg caccctcagg agttcgacag gctggtggaa aaggctcttc tcgccatacc
    20821 gtcccggttt cgccgcagac tgaagaacgt ggccttcgtt gtggaagcgg aaccgcctgt
    20881 tccaaacttg ctcggactct accagggacg ccccttgacc cgtcgaagcg tcggggacgg
    20941 cttcactatg ccggatcgca tcacgatctt ccaggggccc cacgagcggc tggcccggag
    21001 ccccgtccac ctcgaaaaac tcctggaaga tacagtatgg cacgaggtag cccactattt
    21061 cgggatggac gagcgcaccg tccgccatgc ggagcggcgc cgggcctcgc tgcggcgcag
    21121 acgggagccg taaacgggcg cccggcgcct actttgtgct tcccggcaga aaacgcttgg
    21181 gcatcttcgc ggccacggtg aagtgcgggt ccacgatatt cgcgatgtcg tactcaagcg
    21241 cgtggcccat cagcagcgac gcgccacgct cgtcgaaacc gtagtcttcc atcagccacc
    21301 gctgcatttc ggttgtggcg tgctggagcg cctgaagcag cggccgggcg ctgccgagca
    21361 cgacgatttc ctccttgttc tccaggcgcg gccaacggat ggccttcttc ttgatcaggt
    21421 cgacgctgaa ctccacgttc atcgaagttt ccagcgcgtt gccgatgacc tcgccgtcac
    21481 cctgccgcgc gtgaccgtcg ccgacgaaca ggagggcgcc gggctcgtag accggcagca
    21541 tgagcgtgac gcccgccacc atgccgttat agtccatgtt gccgccgaag ttgtcgggcg
    21601 tgctggtggc gatcgcctcc ttattgtcgg gggcggtggc cacgcagccc agcatgggcc
    21661 ggagcggcac ctcgatccgg cgaggacgga tgtctggcga ttccacccac gcgacgccct
    21721 tcgcggtgtc gatgttccag gtgagcgtct ttcgctcccg cagggcttcg gaccggagaa
    21781 aggctgggtc gaccgagtac ggagcgagcg tattgtccga ccacgcggtg gcccggttcg
    21841 gctcgatctt ctcgatccgc accacgagca catcgccccg ctcagcgccc tcaacgaaaa
    21901 acggcccggt ttgcgggttg ggtccctttc ccacctgctt gttctgcgag tcagaaccgc
    21961 gggcgtcgat tgtctgtgtg atgactctgt cgccgggctt gatcttcgcg agcgcggcat
    22021 gcgctccgga gaacgtgttg tggaatccag ccgcctggac gcggtgggtt tcggccggga
    22081 ccggcgcggt ggcaagcgca agaagcgaac atacggtgag aacagcgcag agcggtttca
    22141 ttcgtagacc tccgagatcc tatgcaggat agcactgcgt cgaagaatcc ggagagaagg
    22201 tctactgtaa gcggcctcga cccctcgggc cgggggtcag cggtccgggt tgcgtacccg
    22261 ggcccatgcc ggggcttagc ggactcgttc cttgaaggtt acgttgcccg gtaccacctt
    22321 gcgtaccgcc ggcgccggtc aggcccatgt tcattcgatc ctggcggtaa tcatagatga
    22381 actcccactc ttggtacttc tggcgctcgt tgtagacctt gatgctgtcc gactccttct
    22441 tgctcgccac ccccgcgatc ccgccgccga acccttgcct gccaagggcc tgctgcccgc
    22501 caaggccctg ctgcccgcca aggccctgct gctgcgtgcc tgccaacccg cgtggattcg
    22561 gcgtggtcag cagctttcga ataaggtccg cagcggggtt ttgtcctggc tggctggttg
    22621 actgtgcacc gccaactggt tgtccaccga agaccggccc cccggccggc ccccctccga
    22681 ccagcccccc aacggtagac ggcggcgcgg gcggtccacc aatgaacggc ggagggctgc
    22741 cggaatcgga aggcgacgga gttgggggac cgccgatgaa cggcggaggg ctgccagact
    22801 gcgctccagg ctggttttgt atgggttggc tatagggtac gggcgctccg ggaatccgtt
    22861 gtaccggcag gactgccggc cgcggaatga cttgctgttg gaagggccgt tggcccggtg
    22921 tgccagcctg gaaaccacca gtctgctgct ggccgccggg ctgcggcagg aacgggaaag
    22981 ggggcgcccc gggctgctcg gtctgtggct gctggcctgg aatcgcaggg ttctggcctt
    23041 cgactgtttg ctgaggatac aatacacccg gctggccggt tgcctggccc gcctgcggcg
    23101 cgactggtgc aaaaccttcc tgcgactgcg cttgttgctt actctgcggt tcgtctggct
    23161 ggtcgccgga ctcttcttcg ggcccggctg gctggacttc agcgcccggc tgaggggtgt
    23221 tctcgggggc cgcgacgtca ccggaggcac ccgcgccagg tattcccggc ccaccagccg
    23281 taccgccacg gcgacgcatc cagggggcgg aagtggacgt ctgggattcg acggccgcgc
    23341 caacagacgg gccttcggtg atgaaggtgt tctgactctt cgactcgtct tctttctcgc
    23401 ccggtttctg aaccagagaa tccgtgagta cgcccgcagg cgagatgtga atgagccgcc
    23461 attcgtcgga gccggtcaac gggtctttgt agagccgccg gatgaagcgg atgttgttcg
    23521 ttttttcgag tgctttcagt tccgctggat agcgtttgaa ctttcggaag tatagctgga
    23581 tgccccgccg gtattgctcg ccccggtcga gcagcgcctg ctcgcgggtg cgttggttct
    23641 cgaagacggc caccggcaac tggatgtaga aggagatcgc gatggctgcc gccatgaaaa
    23701 acacgaatag cagggcgaaa cccgactccc cgtgtggtgg tgctttgcgc ggcatcagct
    23761 tgcctctgcc tcaagcttga gcgtttgttc gttcttctgc tcgatgtcct ctactactac
    23821 cgagtttacg ccgatgcgga ccaccttgta gcgtttcttg acgacgtctc cttcagaagc
    23881 gacgtggatc tcgtccccgt cgaggaaaat cgcacgtttg ttcccgagcc gggcctgtac
    23941 ggtgtaacca aagaagcgga gcgggatggg tgtcgccggc tgcttcgccg ctacttcgga
    24001 ccccggcttg gaaggattcg ccggcggcgg gacgggaccg accattagct ttggaatgat
    24061 cttgggttcg ggtgtcttcg ggagcggcgc cgccgagaat tcgaacagac tgcgaggtcc
    24121 tccggaaagg gcgacctttt ctagccggga gatgaggtcc acccgcaggg tcgggtcgat
    24181 ggagctcaaa tccggtcgct cggtaggcgc cctgcgcggg cgcagcgaag ggcggaaatc
    24241 ctgaagcgat cgccggttcg cgcgcggagg ttcggctcgt ggtacaggcc ggcctgactc
    24301 ggcgacggga gccggtgggg gggcggccgg cgactgggcc tgcgacgccg gaaccggcgc
    24361 cgttccgccg ccggagaaga gattgctgta aaggaagtag acggcgacga ccagcaaccc
    24421 accgaggatc gctacttttt tcggttccgc tccgactttc atcgcgccag tggctcctcg
    24481 cggacgaaca cattgagctt catgttgacg ttcagcacgc cctgtccctg ctggggtgtc
    24541 gcctggagcg tatcgatcgt cagaaatcgc ggtgagcggt caatctggtt cacgaacttg
    24601 acaatatcgg cgtacgcgcc ttcgtagttt ccggtaatca ccatcatccc gagtgtgtcg
    24661 ctgccttcga tcggctcggt gacgaaggag tgctccttca tcttgactcc ggatttctcg
    24721 gccagctctg cgagctcgcc gaggatggtg gagtaggcgc ttcgccgggc caggaagtac
    24781 tgttccagga acttgttggc gtcggagcgg gttttctcga cagttgtcac gagcttacgc
    24841 aggtgcgcaa gggaagtctc gcgctttgcc gcgtcggagc taagctgcgc gagttgtcgc
    24901 tgaagatcct ccggcgaacc gccccagggt ttgaagaccg ccgccgcggc caccagattg
    24961 gcgatcagca gaagaccgag caccacccgc acagtcacac gtggatcctt cgccatccgc
    25021 tccccgaaat gcggcgacaa acttcgccac ttggcagcga gaaaaccacg ggctggacgt
    25081 tcttcagagc ttctgggcat agtttacgct cacacggtag cgatacagcg gctccgtctg
    25141 cgccggcggc agccagctat ggacggcggt atccccgaac ataggagagc tttccatacg
    25201 cttcaacatg tccaccacgg gcgtctgcga ctgggatcct acgaccatgt cgaggaggat
    25261 ttcgttcttg gcgttcacct gggggcgaac cgagatcaac cgaacatcgt gcggcaccac
    25321 gtccgccagg tcggcaaaga ttctcgtcca actgatgccc ttccggtaca gcagcgtatt
    25381 gatgaagacg accaggtcga gcacttcggc gttttccggg cgcacgagcg ccgcttccag
    25441 gctcgtctct tctgcggtca tggctcgcag tcgtttctcg atcgtgtcaa ttgctgtgcg
    25501 ggtttccgcc gcctgtcccc gttcaataac ggccagtgag acgagcagca cgagcagcgc
    25561 tgccagcagc acgccgacga acgtggaagc caccaggatc aggcggtccc gccgaaacgg
    25621 ctcactggaa aggttgaggc tgattctcat tctctggtca agccccctcg agtgactcca
    25681 tgtatcctag aagccccgag ttgaattccc ccgtcgtgcc aagcctggaa atgagagcgg
    25741 cgaccgggac cccaagctcg ccttgcagca agtcgtggag gcgatcatcg ttcggctcaa
    25801 acccgcacag caacagcatg tccgggcgcg cggacagttc gtcctcgatg tacgcgaagg
    25861 tcggaaagag gtgcccgacg atctcatgcg cgctcacatc ggccagttcg atcgaccgca
    25921 ccagcctgac cgcaccggaa tccacgacgg cgatgctcag gatccttccc gtccgtttgg
    25981 cgagcacctt cagcccggtc ggcgggacaa gctgcaccgc ggccaggatc gaagtggtca
    26041 cccaacccgg gtgaaggttc gccgcgcgga acggcgcttc atatctcgcg acgacctcaa
    26101 gcggcgccac cgccgcgatg acatccacct tcttcgaatt ctttgcgttc ggctgcggat
    26161 ggaaactaac cgccgccgac tcgacgtcga acggcacgct ccgcttaaga cggaaccgca
    26221 cgagcgatgc ctgttccttc gcgtccgacg ggaacgagtc aaagtcgagt accgaaacgc
    26281 ggacgctgta gtcggggaga atcagcgcgg cggtccggcg cttccgtccg tcgcctccag
    26341 gcgccaccgc cttcacgaga cccaggatcg cgtccgggcg aaggatattg tcgcgaacgg
    26401 gcgaaaccga gatgacgtcc ttctcgaatt cctggaaata gagcacaggc ggtttcccgt
    26461 tctgggcgga cgccatgccg gcctccgaaa gctcaaaggc aaacgcgggc ggcgggtctt
    26521 tcaggaggcc ggtgatggat tggaggaaac tcatttggtg ccggatcagg cttcgatgaa
    26581 cgtgaccttg ttgatctccc tgagagtcgt gacgccgagg cgcgccttct ccagcgccga
    26641 ctcgcgcaag aaagtcatgc cttcttcgcg agccgcgcgc ttgatctcgg cgctcgggcg
    26701 cttgtcaaga atcatctggc ggatgtgctc gctcaggtcg agcagttcgt ggatggcagt
    26761 ccgtccacga aaacccgtgc cgccgcattc gaagcagccg gcgccttcgt agaacgtaac
    26821 ctcgcgccac tcggccaggt cgaggccgct ctccaccagg taggaatcgg gatgctccgc
    26881 cgggcgcttg cagaacccgc agatcactcg caccagccgc tgcgcgagga tgcaattcag
    26941 agccgagacg aagttgtacg gctcgacacc catgttaaga aaccggccga gcacgtcgag
    27001 cacgttgttg gcgtgcacgg tggtgaacac aaggtgcccg gtcagcgcgg cattgatggc
    27061 gatctgcgcc gtctcctggt cgcgtatctc gccgaccatg atcttgtcgg ggtcgtgccg
    27121 aaggatcgag cgcagcccgc gggcgaacgt gaggcccttc ttttcgttca ccggaatctg
    27181 tgtgatgccc ttgatctggt actcgacggg gtcctcgacg gtaatgatct tgtcctcgtc
    27241 gttcttgatt tcgctaaggg cggcgtaaag cgtcgtggtt ttcccggaac cggtcggtcc
    27301 cgtcaccagc accattccgt agggctcctt gatgtagcgg cggaacttcg tcaggtccga
    27361 atccgagaaa ccgacgacgt cgagcgagag tttggcgaac ttctcgctca tcgattcctt
    27421 gtcgagcaca cggagaacgg cgtcctcgcc gtagatggtc ggcatgatcg agacgcggaa
    27481 gtcgatcagc cggttcttgt aacggacgcg gaagcggcca tcctgcggga cccggcgctc
    27541 ggcgatgtcc agttcactca tcaccttgat tcgcgaaatg atcgtcgagt gccagtcctt
    27601 ggagatcggc ggcatggcat agtgaagcac gccgtcgatc cggtacttga tcgcgacctc
    27661 ttgatcgcgg gtttcgatgt gaatgtcgct cgcgcgccgc tcgagtgcgt tgaagatcgt
    27721 tgtgtcgacc agcttgatga ccggggcgat atcactgtca gcggccgtca ggcggtcaat
    27781 ggacagggtc tcgtcctgat tctcttcgtc gcccacgacg tccagtgcga agccctcggt
    27841 cacctcctcg agcacgcgct gagactgttc ggtcttcttg agcagttccg agatctgcga
    27901 cagcgtggcg actttgatcc gcagcttccg gttgagcagg atggcgagtt cgtcgatcag
    27961 gttcaggttg cgtgggtccg ccagggcaat gtcgagtgag ccgttctcgg cgtgcatcgg
    28021 cacgaagttg tagcggaaca tcaggtcgac cggaatggag cggaacagat cgtggtcgat
    28081 gcgagccttc gtcaggtcca cgaattcgca gcggtaccgg gcggccagag agcgcgcctg
    28141 cgctgtctcc tgctccggat ccagtggttt gactcgatca gttgccataa caccaagccc
    28201 ccagcccgcc gtccgccagt cgttgtctgc ctgttacgcg cacggcgtta cgagtcctca
    28261 tttcaatgta tccgccagcg agaagatcgg cagatacagc gccaccaaca caaaggccac
    28321 aaatatgccc atgaaaatca tgatcgccgg ctcgataagc gacagggcgg cggccatgcg
    28381 aatgttcacg tcgtcttcga agaactccga aacgctgttc agcattgccg ggagagcgcc
    28441 ggtcgattcg ccgacctcga tcatgtcgat cgacaaggcc gggaagatgc cagtcgcggc
    28501 aaccgacgac gaaagcggct gcccctctct taccattctc ttcgcttttt cgagcgcttt
    28561 gtgcagcagg cggctgccga gcgactccga ggcggtgtcg agcgcctgaa cgagagggat
    28621 accccccagc agcagcgtgc tcagcacgcg cgcgaactgc gaaacctgat acttgatcca
    28681 gatgcccccg accagcggcg tgcgcagctt gacgcgatcg agcgcctcct gagcggattc
    28741 ccgccgggac cagaaccgaa aagcgaaccc cagcgccaca agccccacgg cgaatgccag
    28801 cacgtaattg cgcgctgtgg tgccgaccgc gatcaggagc tgagtaaccg tcggcaggtt
    28861 agcggacatg ctgctgtaaa gctgcgcaaa gttcggtact acgtaggtga ccaggaacac
    28921 catcagagcg atgaccagca cgatcagcac ggccggatac atcagggaga ccagcagctt
    28981 cttccggatg gcgagagcca gtttctggta ggcaatgtag cgatccagga cctcggccaa
    29041 gctgccgctc ttttcgcccg ccaggatcga cgtcacgtag atgggcggga agacaccctg
    29101 ccgccggaaa gcgtccgaaa gcattgcgcc gttgcggacg tcgtcccgga ccgcctggat
    29161 gtatcgtccc agcttcggat cggtaagcct gtccgccagc agttccagcg ccttcagaat
    29221 cggcaggccg gctcgaacca gcgtgacaaa ctgctggtta aaaatcagaa acttctctac
    29281 attcagcttc ttgcggcggg gcaaggttcc gcggccgatc ggcatcagcc ccgaccgggg
    29341 acggatcgag tagacaagga atccctgatc cgacaaccgc tgccgcaagt catgctcgga
    29401 gtccgcttcg gcgacctgct ggtggatctc ccccctggtg tccgcgtatt tgagcaggta
    29461 ctcggccatg gtcagcgtac ctccccgtcc gcggcaacat ccaccaactc ggcgcccgga
    29521 ggcggctgga accggaacat tcggtcgggc agcggggggt tgagtttctc tcgggagaac
    29581 cggaactcca tcacggaatg gtcctgcccg gtgatattca ggtagcggat ctcatactgc
    29641 ggcgtgacca cgaattcgac cttcgtgtag gggagtttgt tcgatttggg ctccgccgtg
    29701 atgcgcaggt cactaccatc cggtttcgag acgaactttt ggaaatcccg ccagaagtcg
    29761 agcttgccca ataggaaggc gagaggcgcc cgcatgtcgt cgctctcctt caggcccatg
    29821 gtttcgacgc ggttggtcgc cggcgaatag agaaaaacct tcttgccgtc ggagacgaac
    29881 agcttcccct cgggcgaagt gtactgccac cgcatgcggc ctggtctacg tagcgacagt
    29941 tctccgctct cgacgatccg gcgccgtccc tggacgctat atgactgttc aaaaaggact
    30001 tgtatcgttc tggcgcggtt gtaccgaagc tcaaccgatt tcagcagggc gctcaattcc
    30061 tggccgggaa gtagtcccgg cgacaagagc agacaccccc ataccgccca acgggtcaca
    30121 tcatgcttga cggtttcggg cgccaattcg tggatagaac tctccgcaga gcctaggaaa
    30181 agcgcattcc ggagctgaat tcgacatatc tcatggaatt tgttacatta agattagagg
    30241 tctgccccgc gacatggctt ggaaatctcc gtttcagcaa ggcgctcaag tcgaacatcc
    30301 gaagtttggg tttggctcgg tgctgtccag caacgaagag cacatcgtga tcaagtttga
    30361 cgaccacggt gaaaagaagt tcgtcacgcc tatcgtttta ccgaacctga agaagagcga
    30421 tcgccaacca cctgccgaga agcgtgccag ccggagccgg aaaccaaaag tggtggctcc
    30481 cgcagtcgcc ggctgaggcg tccggccaca acacgtccgt atcacgccca ccatgttaga
    30541 ctgagaatct ctcatgagat tcggcgtcgt cgtctttccc ggaagcaact gcgatcacga
    30601 cgcgtggtac gcgacatcgc ggaatttggg ccaggaatcg gagttcatct ggcatgacgc
    30661 ccacagcctc ggcaacgtgg acgcggtcat cctgcccggc ggtttctctt atggtgatta
    30721 cctgcgatgc ggagccatcg cgaagttctc gccagtcatg aaagcggtac gcaagttcgc
    30781 ggccggcgga gggctggtcc tgggcatctg taatggcttc caaatccttg tcgagtgcgg
    30841 ccttctgccc ggagcgctga tccgaaaccg gggcctgaag ttcatctgcc gacaggtcga
    30901 actcagcact gttacgacga attcgccatt cacctgccag gcggcaatag gccaggttct
    30961 ccgcgtcccc atcgctcacg gggagggctg ctactacgcc gatgggcgta ccctcgacga
    31021 actcgaagcg gaggaccggg tagtgttccg atacgtcgac aatccgaatg gttcgcttcg
    31081 cgacatcgcc ggcatcctca gccgcgagcg taacgtcatg gggatgatgc cccatccgga
    31141 gcgggcctct gaccccctga tggggtgtac ggacggattg gccgtattca ggtccatggt
    31201 cgccgcgatg gcggtgaagt gagatgagtg ccatcactcc cgaggtagtt gcctcccacc
    31261 aattaagcga gtccgaatac gacaagatcg tttcgttact cggacgcgag ccgactttca
    31321 cggaactcgg cattttcagc gtgatgtgga gcgagcactg ctcgtacaag agctcccgtc
    31381 tccacctgaa gaagcttccc acgcgcagca aactagtgct gcaaggtccc ggagagaacg
    31441 cgggaatcat tgacatcggg gacggttacg cgatcgcatt caaaatcgag tcccacaatc
    31501 acccgagttt catcgagccg ttccagggcg cggccacggg cgtcggcggg atacttcgcg
    31561 acatcttcac gatgggcgcc aggcccatcg ccgtcatgga ttcgctccgc ttcgggccgc
    31621 tcgacgatcc cgaagtcggc gcccgaaacc ggcgcatcct ggagggagtg gtcgcgggga
    31681 tcgcacacta tgggaactgt ttcggcgtcc ccacggtcag cggcgagtgc gtgtttgaag
    31741 agagctacaa cggcaacccg ctggttaacg tctttgcgct cggcgtgttt cgcacggacg
    31801 agattttcta tgcgaaagcc agcggcattg gcaaccccgt gatctatgtc ggcgccaaga
    31861 ccggacgtga cggcatccac ggcgcatcga tggcctcggc cgagtttact gaagaatcaa
    31921 agcagaagcg gccgaacgtt caggtcggcg atccgttcct cgaaaagctg ctccttgaag
    31981 cctgcctgga agcgatgcag actggcgcga tcgtcggcat ccaggacatg ggtgccgccg
    32041 ggctcacctg ctctacgtgc gagatgggca gccgcggcgg taccggcatc gaaatcgatc
    32101 tcaagtacgt cccgcagcgc gaaaccagga tgactccgta cgagataatg ctttcggagt
    32161 cccaggagcg gatgctcctt gtggcggaga agggccgcga ggaagaagtt ttcgctgtct
    32221 tccgaaagtg gggtctggac gcggtgacca tcggtgaagt caccggcgac ggactcttgc
    32281 gcgtcaggga ccgcggagtg gtggtcgccg agatcccgaa cagcgccctg gccgacgaag
    32341 cgccgatgta cgaccggccg cacatggcgc cgctgcggaa agctccgatg gagggcccgc
    32401 ccttcgccag cggagacctc caggcggatc tcgcaacctt gctcgcagcc ggcgacatct
    32461 gttccaagcg ctggatcttc gagcagtacg atttcatggt ccggacgaac accgtccagg
    32521 gacccggcgg ggacgcggcc atcgtgcgga tcaaggaaac cgatagttcg atcgcaatgt
    32581 cactcgatgg cagtggccgg tactgttcgc tctcgccgcg ggaaggggcg aagctggcgg
    32641 tcgccgagtg ttgccgcaac ctctccaccg tcggcgcgct gcccgtcgcc gccacgaatt
    32701 gcctgaactt cggcaatccc gagcggccgg agattatggc gcaactcgtg gaggccatcg
    32761 agggcatggg cgaggcctgc cgcttcttcg atacaccgat caccggaggc aacgtcagtc
    32821 tgtacaacga aacgctcggg gaggcgatcg accccacgcc ggtgatcggg atcgtcgggc
    32881 ttctgaagac cggggcgcct ctgaccatcg acttcaagca gcccggtcgg actgtggtcc
    32941 tgctcggcgg tctgggtaac tgtgacgacg cgcggttcgg tggcacccag tacgccaagg
    33001 tggtgttgaa cgagatgtgg ggcatgcctc ccgcgctcga catggcctac gagaaacgca
    33061 ttcatgcggc gatgagggcc atcgcggagg cggggcttgc ggagtcggcg cacgacttga
    33121 gcgacggcgg gctggccgtc gcagccgctg agtcatcctt tggacctgtc gcggtgggcg
    33181 ccgaactggt tctcgacagc ggcttgcgcc cggagtttct gctctttcac gaagctccct
    33241 cgcgcatcct gctctcgacg agccggcccg aggaagtgca gcgcattgcc cgggagcact
    33301 cggtcgaagc gccgcttgtg ggtgcaaccg ttaaggggag gctctccatc accaaccgag
    33361 gcaggaccct ggtagactgc gaagtggggg agctgcggag cctctgggaa ggggctctcg
    33421 agagagcgct tcacgcctga gccatgcaag accagttcca cgacgagtgc ggagtgttcg
    33481 ccatctacgg gcatcccgag gcggccaacc tgacgtacct gggcctctat gcgctccagc
    33541 accgcgggca ggaaagcgct gggatcgcca ccagcgacgg aaccgagatc aactgctaca
    33601 aggccatggg ccacgtggcc gatattttta cgcctgccgt gcttgcggcg atgcccggcg
    33661 atgcagccat cggacacacg aggtactcga ccgccggcga cacggtgctc ctgaacgccc
    33721 agccgttcag cgtagcgtgc aacaagggcc gcatcgcggt ggcgcataac ggcaatatca
    33781 ccaacgccgc cgacctgcgc cgcgaactgg agcgcgaagg gtctattttt caagcctcca
    33841 gcgacaccga ggtgattctt cacatggtgg cccgttctcg ggaacggacc ctcgccggtg
    33901 cgctgcgcga ggcgctgctt cagctcgagg gggccttttc cctcgtcttc ctggccgaag
    33961 acagggtcat cgtcgcacgc gacccgcacg ggtttcgccc gatggccatg ggccagatgg
    34021 agctttccca cggcaagatc tgctatgtct ttgcgtcgga aacgtgcgcc ttcgaccttt
    34081 tgggcgccgt ctatctcaac gacgtagagc caggcgagat ggtgatcgtg gggccggaag
    34141 ggctgacccg agaacggtac gccccggagc agccgaaggc gcaatgtgtc tttgagcatg
    34201 tctacttctc gcggccggac tcgatcgttt tcggccggtc ggtgtccgag tcgcgcgaga
    34261 tgctcggacg actgctggcc cgcgagcacg ctgtcgacgc ggacgtcgtc gtaccggtgc
    34321 cggactccgg cgtggcggca gccatcggtt actctgcgga gtccggaatc ccgtaccggc
    34381 aggccctgat ccgcaaccac tatgtcggac gcacgttcat cgaaccttcg caggcgatcc
    34441 gcgactttgg ggtcaagcta aagctgaacc cggtccgtcg cctgctcgag ggtcagcgag
    34501 tggtgctggt ggacgattcg attgtgcgcg gtaccaccag caggaagatc gtgcgtatgg
    34561 tccggagcgc cggcgctcgc gaggtgcatc tgcgtatctc gtgcccgccg acgatttcgc
    34621 cctgtttcta tggcgtcgac acgccgacga agaatgagct gatcgcttcc aacaacaccg
    34681 tcgaggagat ccgccggttc gtcgaggcgg attcgctcgg atacctctcg ctctcgtcac
    34741 tccgtgaagc ggtccacgac gacaaacacg aatactgcta cgcctgttac acgggcgact
    34801 atccgaccga cctcgtcaat atcgaggatc tcatcgcggc caaatcgcgc cgcggctgat
    34861 caatgactcc aaaggtcgct tcctccgcgc tggccgtgcc gcactcgcgc atccgcgaac
    34921 tggccgaggt cgcgatgtcg atggacgggg tgctgaagct ctacttcggg gagtcgaacc
    34981 ttccaacacc ggagtacatc aagcaggcgg ccgttcaggc gctctctgac ggctatacgt
    35041 tctacaccga gaatgccgga ctgccgtcgc ttcgccgcgc gctcgccggc tactaccacc
    35101 gagtgcaagg cgtggaactc gacccggaac gagagatcgt aatcaccgcc tccggcgtgc
    35161 aggcgctttg cgtcggcatc cggtgcgtgc tggaccccgg cgacgaggcg atcgtgctca
    35221 ccccggcgtg gccaaacggg tctgcgagcg cggccctgtc caacgcggtg gcgaggcagg
    35281 tcccgcaccc gctttgcggt gagcgttaca tgatcgactt cgccgcgctc gaggcagccg
    35341 tcacgccgcg gacaaggatg ctgctttaca cctctccctc gaatccgctg gggtgggtgg
    35401 cgaccgggga cgatcaacag cggctgctcg gcttctgccg ccgtcacggg ttgtggctaa
    35461 tggcggacga agtctatgac cgcctctact acgagggtgg cgcgccgggc gatccggtcc
    35521 cgtcgattct tcggatgacg acgcgcgagg atgccgtcac ggtcgtacag tcgttctcga
    35581 agtcgtattg catgacgggt tggcgcgtcg gctggctggt tgcgcgtgcc gacgtcgcct
    35641 cacgggcgac acagttgaac gagttcatca tctcccacgc gccaagcttc gcccagaaag
    35701 ccgccgaaac cgcgttggcg gagggcgaag aagaactcgc gcggatggtg gggcgtctga
    35761 aggataaccg ggacttctgc tgggccacgc tccgcgagat gcccggtatc accgtcccga
    35821 agcccgaggg cgcgttctac ctctttccga gaatcgacgg cctcaccgat tcgttcgagt
    35881 tctgccgccg cctgcttctg gaagagaaag tcggccttgc tcctggtgtg gccttcggag
    35941 ccggtggtga gggctcggtg cggatctgct atgcggccga acggtcgatc ctcgaagagg
    36001 cgatggagcg actcggccgc tttctgcgcc gcggggtcta agacgagaaa acgaatgacc
    36061 tcgtggatca gcaggccacg agaagcgcta gcctagcgga agtcgggcta gtgcaattcg
    36121 aattggctcc ttagcttttg ggtggcggct gatcggagtt gaaaatatca acgaccgctt
    36181 tccaagagcc gttggcctgc ttcttccaaa cagtcgcgta tttgcccagg tccttcaccg
    36241 gctttccttt cggatcgttg acggacattt cataggagcc gtaggtatag gcgagatcgc
    36301 ccgagcgcgc cacttcgacc ttgaccgtct gccagcttag tgcaaggctc ggcaacgcca
    36361 gcatctcaga ccacgctttt cggatcgcct ctctgcccgt tgcgatcgga gcattaggcg
    36421 tgagcatcga ggcatcctcc gcatagtagg aaagaatgcc ttcaagatcc ttggcgaatg
    36481 cgcggttgga ccactccgtc tctgcaacac ggattgcgct ttcgtcagcc gcccgggtgt
    36541 cgggcggcgg ggctggttgc tgttggcagg ccagcgtgac aaccaggagc agcagcaaca
    36601 ctgcgacgtg ggttctaaag tgcacgggac accatccttt ctgtcattgt tcccagggaa
    36661 gcgatgtact tgcagcctag ccaggattcc cgttttggct tagttgctgg cgtccattgt
    36721 acatctaatg gtccggttca ccagtgaact aagcgcgaaa acggggaggc ctgtaggctt
    36781 cgagagcgtt cttccagaag tagctgtcga ccgcgtcctg tcccttggcg gtaaagtact
    36841 cccggaccac tctcaagacg gcatcgtacg gcgccaccag atcgctgacc ggccagttac
    36901 tgccataaat caggcggccc ggaccgaaga cttcccagag ctcatcgagg gccggacggt
    36961 acgcactcag ttccgtgggt gctttgcttc ccttgcggcg caggaccgac gaaaccttca
    37021 tgtacaccag cgggcgcttc ccgacttcgc gcagggcgcg gtggaacgcc tcccgatcgg
    37081 acgcctctgc cggcggatcg aacgggaggt gatcaatgac gattcgaaga tccggcacgc
    37141 cgtcggcgag acggagcaga tcgtccagca ttgccgggcc gcccacggcg tcgatttcga
    37201 gcccggcggc tgcgatgcct ttgaggtcct tcagaaatgg tgtgtgtgat agcccgtcgg
    37261 cgatcacctt ggctgcgagc ctgatcccgc ggaacagggg gttcttccca aaccgctcta
    37321 ggtggttctg aaacgcaggc ttgccgggtt cgaggtggcc taccagtccg agaatgacgg
    37381 gatgcttctc ggctacgtcg agtacccact ggttgtcttc gagccacggg ctggcctcga
    37441 ccacgatggt tcctgtcacg cccatccgtt tcgtcaacct gacaaattcg tccgggagca
    37501 ccgtccggta gagaacggtc tctcccttgg aaggccacgg cacgccttcc ggccgtgacg
    37561 ggtcatagaa atgcgtgtgg gtatcgatga tggccgctgg cgaaacgact gaatctgccc
    37621 aagccagagg caagcttccg gtaacgccga gaaacgctct gcggttcatg gcaacgtctc
    37681 agagtttaca ggaattgtcc ggtccccgac cggaggagga agggcctcag ccacccgtga
    37741 aacggctcag gatgagccag ccggcaacga tgagaatgat ccagatagcg tccacgaatt
    37801 gacctcggct ttcattctac gtgatatctg ccaaggttta cggcgagatc atcaagtagg
    37861 ttgccgcccg gaaggcagcc tcaccggcta cttcggaaag cctgcagggt tgatgccggg
    37921 aatcacgcgg agcgcgttct tatagtacag cttcttcagg acctcgtcgg ggagctccag
    37981 gccgtacatt ttccagaacg cgtgtcgctt gcggtagtag tcgaagtact catcggcggt
    38041 ctcgagcacc cggaagtagg tgtggtactc cgtggcgttc caagagtcct tgccgaacat
    38101 cacccggtcc tggtgacgga tgaaccactc ccgggcgaaa cgcggctgcc gccccagttc
    38161 agccagcacc gcgccgatct cggtgtacat gttgggaagc gtgtccatca ggcggcccaa
    38221 ctcagcgagg ttgccgccga gccagcccaa gtgggcgttg atgaagatcg tcttcgggtg
    38281 cttcgcgaac atcttgtgct gctcgcccat gatttgttcc cagctaggaa agcggtctgc
    38341 cggccgggcg cgcgacggga tctccttcaa ctccagccag cgttcattga accgatcgtg
    38401 cggctggaag aaagaccatg gctcgccggt gtgaatcagc acggggatac cgagcctccc
    38461 acaggcctcc cataccggat cgaaccgcgg gtcatccact gggatgcggc ggccctgtga
    38521 gtctgcgagt gtgagtccga gattcttaaa aatcttgagc ccctgcgcgc cgttcttcac
    38581 atcctgttcg agttgcgccg cggcgcgctt gccccaatca ggatcgtcaa tccccgcgaa
    38641 attcaggttc gcgaagatta cgaagcggtc cttgtaccgg cctttcatgt tcgcgacgcc
    38701 gctctggagg cggtctccgc tgcccccgct taggttgacc attacgcgca tgttcaggcc
    38761 gtccatgtcc ttaaccagcc ggtcaaggct gtctgccggc atctgcgagt tctggtggct
    38821 gtgaacgtcg atgaacggat acctcgcccg cgtgagcggg tgctcgcgca caactagcgt
    38881 cgatttcgga tcgtattcct cgatcgacat cgtttgcgcg aatacggaag ctgcgcagag
    38941 aaagcagatc ggaacgtatc tcatggaacg ttggcgttct ttcgagtata cggcgctcag
    39001 gaagcgttgc gcaccgtctc gccgagtagc tcgaggatac ggtcgcggat ctccgtcgta
    39061 agccactcgc gatcgcgcag ggtgagtccc gccgtcggga tcgcgtcgcc gatctggagc
    39121 cggactctgc ccggtctgac gaccaccgaa ttcatcggca ggatctcccg ggtgccctga
    39181 atggagaccg gcacaaccgg caccccggcc ttgatcgcga tgtaagcggc gccctcctta
    39241 aagtcgttca gtccgccggt ggcggagcgc ccgccctcgg ggaacaccag ggccgagact
    39301 ccctgcgcct gtaccatccg cgctgcctcg gacatcgcct tcagcgaacc acgcggatcg
    39361 tgccgcggaa tcgggacgtg tccggcccgc tttaggtggc ccccgatgaa cggaactctg
    39421 aacaggccct tcttcgccag gaatcggaac tgaaccggca agtaagccat gaggagcggc
    39481 gtgtccatga ggctcaagtg gttcgacgca atcacgtaac ttcccccggg cgtgatctta
    39541 tcgagacctt cggcgtgaac gcgcgcgccg ctcagcaaca gcagcatccg cgaccagcaa
    39601 cgggcaatcc ggtgcggcag attgccgctc cggtcataga gggacgcgaa gaaggagagc
    39661 gtgcccatca ccgcggtcgc caggacgatc agcggatcgg ttaggagcag cgctcgaaga
    39721 gtggacaccg ctccctattt tacgaggagg gcgcgggcct cgcccaccag aaacagcgtg
    39781 ccggtgatga aaaaagcatc ctgggaggca gcgccgtcca tgaacctcag cgcctcccgc
    39841 aggttcgcgg tcacctggat gcaggggtgg tccaccagtt ccctcagcgc ctgcggccgg
    39901 atggagcgcg ggctgtccgc cgcggtaaga atcacctcgt ccgccagcgg gaagagaatt
    39961 cccgccattt ccgtgacggc cttgtcgcgc atacagccgt agatcaagcg gacgcggcgg
    40021 ccgtgataga agcggcggat gtggccggcg agcgcgcgtg cgccggatgg gttatgggcg
    40081 ccgtcgagga tgatttcggg ctgttcggag acacactcca gacggccagg ccaagcggca
    40141 cggcggatgc cctcggtaat gcgatcgccg gggactccca tttcatacag cgccgcgacg
    40201 gccgtgagag cgttcatcac ctggtgctcc ccggcgagcg ggcactcgac cggaatgcgg
    40261 gcggctccta cggcgagaaa tcgacttccg cgacggtcga aatcaagatc gcggacctcc
    40321 cagtctcccg ccggcctgac actcgtcccg gccaccgcgg cgcgctcctc gagaaactcc
    40381 agaattccag gccgctgcac cgcgattacc gccggcactc cggacttcag gatgcctgcc
    40441 ttctccgcgg cgatcgcctc tatactgcgg cccagaaacg cctcgtggtc gaagtcgacc
    40501 ggcgtgatta cgcaaagtgt tggggttacg acgttggtcg catccaatct tccccccaga
    40561 ccaacttcga gaacggccgt ctcaacgccg agatcccgaa acagcacgaa ggccatggcg
    40621 gtgaccgtct cgaaatacgt cggatggaaa tcgagggagc cctcggcgag cagtgcttcc
    40681 gcctcacggt ggacctggtt gaaggctctg gcgaaaagcc cggggtccac cggctcgccg
    40741 tcgatcctga tccgctctgt cggttcgacg aggtggggtg atgtgaacag tccggtccgc
    40801 tgtccggcgg tccgcagggc cgattcgatc atcgcgcaga ccgaaccttt accgttggtc
    40861 ccggccacgt ggacgaaacg gcaggcgcga tgcgggtctc ccagacgggc gagcagcgcc
    40921 gcgatgcgat cgaggccgag tttcgctgtc ttgacctcgt ttccgagcgc gtaaagaaac
    40981 cgcaccgatt ccggatagtt caccttggga gccgctcagg tttcagaata accccctgcg
    41041 aatccgtccc gccgtgggag gcccgtgtta cactcactgg tatattgagg gcagctttcg
    41101 gtcgagcaat gccgcggatc acgccttgac atgggaggtc tttgatgagg gcaacatcga
    41161 gaatcgagtc gctcagttac gggaccattt acatgaccgg gtgcatagtc gcgttgttcc
    41221 tgtcctttgg acaacacagc ccgctccagc gtgttctcgt ccacacggtc tgttcttggg
    41281 gctacgtcat ctactgggtg ctcaattacc agtagattct cctccgttcc cggggttctc
    41341 ccctcccgga ttttgcctta cactgaggtt ggacttcctc gattggactt catggcacgg
    41401 tgtggcatcc gtgcggggag gttggtatgc cgatgttgca gaagttgctg gcctacttgg
    41461 acgagaagca ggtacagtac acgcatcacg ctcacccgac tgcgtacacg gcgcgggaag
    41521 tggcctcggt ggaacacgtt ccggcgcaga caatcgctaa gacggtcgtg tatttcagcg
    41581 agaacggcta tggtgtcgtc gtgctgcccg gcgatagcat ggtggatctc catgcgctga
    41641 gagagtcgct tggtgtggcc aggctgcggc tggcgaccga aagcgaactg ggtgacctgt
    41701 ttcccgactg cgagttggga gcgatgcctc cattcggcaa tctgcgtgac ctgccggtat
    41761 acgtggacgg caggctgccc gccgaggaga cgattacctt caacgcgggc acgcaccggg
    41821 acgtgattca gatgaagtat gccgatttcg agcggctggt gagacccgcg gtactgacga
    41881 tcagcaaaca cgcagcggcg tgacactgct aggtgaattc ccacgagcca cgaaccgctg
    41941 tcagactttt tccgccttcc aggcgtcatt ctacaaggag ccggagactt tgatctttcg
    42001 ggaagttgaa gaccccgacc tgatcgcaaa agcgcgtcgg gggaaggtcg aggcctacaa
    42061 cctcctcatt tcacgatggg agaagcgtgt tttcaattac ttgctgaggc tggtcggcga
    42121 tcgggaggat gctctcgacc tcagccagga agtcttcctc aaggcctacc agaacctggg
    42181 aaagcttcag gacccgacgc gcttcgcccc ctggttgttc cggatcgccc acaacgaggc
    42241 gttctcccat cttcgccggc ggaggccgga cgagccgttc gaggtttttc cggtcgagtc
    42301 gggcgccgcc actcgtcagc cgccgatcga gttgacactg gctgtggaag cggcgcttcg
    42361 ccggttgtcg gaggagcaac gcgaggcggt tttgctgaag atttaccagg gattcaagtt
    42421 cgaggagatc gccgagatcc tctcttgtcc ggtctcgacg gtaaagtcgc gggtttattc
    42481 tgctctcgac gtcctgaaac agatcctggc tccaattgac acgcggggtg tggaatgagt
    42541 tgcactcagg tggatttgaa aggtttcttt ctcaacgagc ttgtgccgga cgcccggcag
    42601 gccgttgagg agcatcttcg ggtatgcccg aattgccggg aagaattgga gcggctgtcg
    42661 gtgacgcgcg ccgctcttac gtcgctgcgc gaggaggaga cgccgcgccg gatcgctttt
    42721 gtctccgaca aggtactgga gcccagcatc tggcggcgtt tttgggactc ggcgccacgg
    42781 ctcgggtttg cttccgcggc gatgttgtcg gtcgcgctcc tggtcaacgg attcatccgt
    42841 ccgcagccgg cggcgccggc gcccatggat agcgccgccg tgcaggcgaa gattgaagcg
    42901 gtggtcgccg gacgtgttgc agaggcggtg cagaaagcgg tggccgattc cgaagcccgt
    42961 tcggctcggc ggacggccga cctgctggcc gcaaccgaac agcgtctcga ccggcagcat
    43021 cgagccgatc tgctcacggt cgaagataac ttccgggtgc ttcaaaagcg agtgaacatt
    43081 tactaccggg ccagcaacga ccttgggagc gctcaatgag acgggctctt tgcatcgcct
    43141 ctgtgctggt tgcggcggct gccgccggcg atcctccaaa gccgcgcgta acccgagcgg
    43201 agcttgcggc ggtggagacc agcttcgacc agcgcatcgc cagactcagc gtcgcagatg
    43261 atcccatgga ggtgctcgga tcgacccgcg gagtctacct cgagggctat ggcgccgtat
    43321 tcaccaccga ggtgaacctc gtgatcacgc cgcccatcac cccgttccgg atgaagatca
    43381 ccaaggaaga ggtagcccgt gtccaccaga ggaaactcga gcggctgccg acgctgaaac
    43441 gcgcgatgcg cgcgatgctt gtctcctcgg ctgcttcgct cgacgacgtc cccaccaacg
    43501 agcaggtcgt cgtaggcgtc accctgtttt tcttgaactg ggaggacagg acgggtctgc
    43561 cttcgcagat cgtcatgcag gcgccccggc gtccgcttct ggagtacaac ctcagcacga
    43621 gcaccagcga ggcgctggaa gcggccatcc gcgagcaggt gttctaaacc atgcgtctcg
    43681 gagagatgtt gattgcccgc cggctgatcg agccggaaga cttggaacga gctctcgaga
    43741 tccagcagga gcgcggcgag aaacttggca agatcctcgt tgaccttggc ttcgtcgcta
    43801 tgcgcgacgt gctgtcggcg ttggccgagc agcttgccgt gccccttgtg acgatcgaca
    43861 gcccgccgcc cgtctcaccg gagattgagg gcctgagccc gcgcttcatg cggcagtgcc
    43921 gcttcctgcc cgtcgccatg agagactcca ctctcaccgt cgccatggcc gatccgctcg
    43981 acttcgagac cctgtccgcc gtgggcggct tcaccgggct gaaggtcgaa ccggtgctgg
    44041 ccgcggaaca ggagatcctc gacggcatcg acaagttctt cggagaagcg gagaaaggcg
    44101 ccgccggctt cgacgaggac tcggcccagg cggccgagga tctcgaacac ctgcgcgaca
    44161 tggccagcga ggcgccggtg atccggctgg tcaacgcgat gatcgcgcag gcggtcgaga
    44221 agcgatccag cgacattcac atcgagcctt tcgaaaagga attccgcgtc cgctaccgcg
    44281 tcgacggagt gctctacaat caggagccgc cgccgcgcga actgaaggcc gccatcatat
    44341 cccgcgtgaa gctgatggcg aagctgaaca tcgccgaacg ccggctgccg caggacggcc
    44401 gcatcaagat caagacactc ggccgggagg tggacctccg cgtctcgacg ctgcccaccc
    44461 tgtacggcga gagcgtggtc atgcgcttgc tcgaccgctc ggccggcgat ttctatgatc
    44521 tgcgccgcct tggtttcgat gagcacatgc ttagccggat ggagcactac acctcgctgc
    44581 cgcacggcat cctgctcgtc accggaccga ccggtagcgg caagtcgacg actctgtatt
    44641 cggcgctcaa gcgaatcaat cagccggaca agaagatcat caccatcgaa gaccccgtcg
    44701 agtaccagat ggacgggatc aatcagatac acgtcaatcc ccagatcggg ttgaccttcg
    44761 cctccgggat ccgccacatc gttcgccagg acccagacgt catcatgatc ggcgaaatcc
    44821 gcgaccgcga aacagccgac atcgccattc gcgcggcgct aaccggccac ttcgtttact
    44881 ccacactaca caccaacgac gctcccagcg ccatcacccg tctcagcgac atgggggtgg
    44941 agaactacct cctctgttca tcgctcgtgg cggtactggc gcaacgtctc gtgcgcgtta
    45001 tctgcgaaca ctgcaaggcg ccggacagcg tacggaccgc acccgatggc cgtccgcttc
    45061 gctgctggcg cggcaaaggc tgcgagcgct gctttggcac cggcttcacg ggccgagtgg
    45121 gcatcttcga gctgatggag atgaatgaag aaatccgcag cctcattatg aaagacgccg
    45181 acgcagccca gatcaccgtc gccgcgcgac accacgggat gcgtaacctt cgcgaagacg
    45241 gctggctcaa ggtggagtcc ggcctcacca cctgggatga ggtgatgcgt gtgactcagg
    45301 agttctgatg gccagcttcc atttcagggc ggtggccgcg gacggaaaag tccggaccgg
    45361 cacgctatcc ggcgagaccg agaaaacggt ggcgatggag ttgcggcgcc agggtctcgc
    45421 gcccgtgtac gtcgggctgc agccgaagaa ggcttttcag ttgcggctgc cctcctttgt
    45481 ccgcggccgc cggaaggacg tgctgttctt cacccaggag ctttccacgc tcctcaacgc
    45541 cggtgtgccg ctggaccgcg ctctggcaat caccgcggag ctgggtgagc gtccgaactt
    45601 ccgcttcatc gtgtcggacg ttcttcgtgt tcttaaaggc gggaaatcgc tggcggacag
    45661 cctcgccacc caccccgagt acttctccga gctgtacgtc aacatggttc gagcgggaga
    45721 ggccagcggc gcgctgtcgg tggtcttcga aaggctggcc gagttcgaga actcacgcga
    45781 cgacctgcgg aattacatca tctcgtcgat gatctacccc gctctgctgt cgctggtcgg
    45841 cctcggatcg attcttgttc tagtcaactt cgtcgcgccg cgcttcgcgg cggttttcca
    45901 ggagtcgcga atcccgatgc ccctgccgac tcgcatgatg ctggaggcga gccgtctgct
    45961 ccgggcatat gggtggatcg ccgtgctcgc cgttgccggc gcgggggccg tattctactc
    46021 ctacatccgc accgccgtgg gccggctttg gtgggacacg ctccggctcc gggtaccatt
    46081 gctcggggga gcgcttcgtc gcgccgagac ggctcgtttc gcccgcgcga tggggacgct
    46141 cgtcgccaac ggcgtcggcc tggtgcaggc gatcggtatt gcgggcgcaa tcctgaataa
    46201 caggcgaatc gccggctcgc tcgacgcggt ggcgcagggc gtgaaacgcg gcgagggcat
    46261 cgccgcgccc ttgtcccgtt ccgggcagtt tcctccgctc gccggtcacc tcctcaccgt
    46321 tggtgaggag accgggcgcc tggaccagat gttctcccgc atggcggaga tatacgagaa
    46381 tgaaacgcgg gcggcgatca ggcggttcac tgcgttattc gagccgatgg tgatcctggt
    46441 gatgggcgtg attgtgggcg ccttgatcct cagcatgctg ctcgcaatca cgagcattaa
    46501 cgaggtggcg gtgtagatta tgagcgatcg caagaaacga aaacaagccg gcgtcacgct
    46561 gatcgagatg ctggtcgttg tgacgatcat cgcgctattc gcggcactgg tcggccggaa
    46621 tatcttccgc cagacggaca aggcgcgcat cacggcggcg cgcgcgcaaa tcgaaatttt
    46681 catgggggcg ctcgggcagt acaagctcga taccggcggc ctcccgacta cagatcaggg
    46741 actgctggcc ctctggaccc gtcccgcgga cgccccaccg agctgggacg gtccgtacct
    46801 cgcgaaagag gtgccgcccg atccctgggg caacgcatac gtctatcgct ttcccggcga
    46861 gcacggcgac atgcccgaca tcgtctcgta tggcgcagac gggcagccgg gcggcgaagg
    46921 ctttaacgcc gacatcatta gttggaagag ccagtgaaac cgtctggtga tctgttgaac
    46981 gcccgcttga gaggccgccg agccgcgagc actgtcgacg tgcaggcgcc ggaatcgaca
    47041 ggccgggaaa tcaacagatc tcagagtggt gtcaccctga tcgagatgct catcgttgtg
    47101 gcgattatca gcctcatggt gggcatcagt tacccatcca tttcctccgg agtcgatgcg
    47161 ctccggctct cgtccgcgtc tgattcgatc gcgagcttcc tgaacggcgc cctcaaccgc
    47221 gcggagcgga agcaggttgt ggtcgaggtg acgatctcca aactagagaa cgccctgatc
    47281 atgcgttccg aggagccggg tttcgaacgc cgtcttgatg taccccaggg agtgcggatc
    47341 accggcgtgc ttcccgcgaa cttggtcgat cccgatgctc cccgccattt tctgatgtat
    47401 cccggcggga ccttgccgag attcggcgtg gaactcgcca accagcgggg cggccgacgg
    47461 atcgtccgcg tcgacccgat taccggcaat tcgcaggtgg agcggccgga agcccaatga
    47521 gagaacgcgg tttcacgctg cttgagatgc tggtcgccac ggtgatcatg ggcgtggcgg
    47581 ttgtgggact gctgtcggcc atctcggcgt ctctgcacaa cgcttccagg ctcaccgact
    47641 acgatcgcgc agcactcgcc gcgaagcgaa agatggacga actgctgctc gactcccgcc
    47701 ttccccgcct ctccgtgctc gagggcagat tcgacgcgga accgggaagc gggttcgaag
    47761 gcggctggcg agcgaggctc gcgccctatg agatgccgcc ggcgacgggc ccgagcacgc
    47821 tcgttttgga gcgcctcgaa ctggaggtct ggtggatgag cggcgggcgc aggcgcacca
    47881 tcgctctcga gggtttccga tcctccacgg cggccggtct tgcgcccgcg gagggccagc
    47941 catgaggcga acccacaccg ccggtgtcac cctaatggag ttgctgatcg cggtgtccct
    48001 gttggccctg cttgccaccg gaattctggc cgcgatgagg atcggcttca acgcgcttgg
    48061 caaaacaaac cgcaggttga tggacaaccg gcgtgtcgcc ggcgcgcagc gcatcctgga
    48121 acagcagatt gccggtttca ttccggttgt cgccgattgc ctgcccgatc ccgaacggcc
    48181 gcccgtgcca gcgccttttt tccagggcga accacaatcg atgcgctttg tctctgccta
    48241 ctcgcttggg gaggcatggc gaggattacc gcaaaccgtt gaatttacag tggttcctgg
    48301 aaaggacggg agaggtgtga tgctcgtggt caacgagcat ctctacacgg gtcctcgcgg
    48361 tcctggactg ttctgcctcg gctccgagtt cgatcccgaa ctcggccgcc cggccccgcg
    48421 ttttcgtccg atcgaggtcg gaccccgctc gttcgtgctg gcggataagt tggcattttg
    48481 ccggttcgcg tatcgggaac tgatgccccc tccggtgctc gaacgctgga ttccgcgctg
    48541 gacggcgccg cagtggccga caggggtacg gatcgagatg gcccccctgg agcacgatcc
    48601 cgcgcgcctc cagccgctta ccctgaccgc gccgattcgg gtgaaccggc ttcccgggga
    48661 ggcgtatgcg gactgaatgc cgtgctgaaa aacccgccaa cggcattgcc atgcacagcc
    48721 taaaggctgt gccaccgttg agaatcagca gcctacggcc tgcagtggca caggcttcag
    48781 cctgtggcgc gggtttttca gcgccctgtt taggctgtgt gccggtctcc cgacgcggcg
    48841 gcgccctgct agtcgtaatg tggctggcag cggccctgtc agccatcgct ttcacgctcg
    48901 ccgggaccgt ccgtaccgag accgaacgca cctccaccgt gcttggcgga gtgcgggcgt
    48961 actatctggc caccggagcc atcgaccgcg cagcccttta catgctctgg ggccggcagt
    49021 tcacgatgcc ggacggctcg ccccgctatt acgcaccctg gatgaacgct ctgcagttct
    49081 ctttcgagag cggggaagca cgagtcgaga ttatcccgga cacgagcaag atgaacatta
    49141 acaccgcgcc gccagaggac ctcgttcaac ttctgcttgc gcttggcgcg gaccccgagc
    49201 gtgcaaatct ggtatcgcag gcgatcgtgg attggcgcgc cccgcgggta aatggtgagc
    49261 ctggcccgtt cgaccagtat tatctctccc tcacgccgtc ttttccttcg cgtcatgcgt
    49321 cttttcaaga ggtagaggaa cttctgcttg tgagaggcgt cacacccgaa cttttctatg
    49381 gtggcttcgt tcgtagcccc gagggacgct tgatccgcac gggagggctg cgggactgcg
    49441 tttccgtaac cggttcgtcc cgtgagttcg acgtgaacac cgctcacccg gccgtgctcg
    49501 cggcgtcggg acttgacccc gggacggtgg cggcgatcgt cgagacacgc agccggatgc
    49561 cgttccgctc ccaagaagat ttgaacgctc tagggattcc gcctggcgcc cgccgcctcc
    49621 gcatcggtgg caacaggatc ttcacgctgc gagccaccgc ccgcctccgg gtgtctgaca
    49681 aggaattctc cgatcttcgg cggtccgtgg cggcgacagt gaagcttgag gcctcgcctg
    49741 acgggcgtcc ctaccgggtg ctccgctggt acgacaacgt ttgggtgcaa tgactgctca
    49801 cgacgtaaga agaattctgg ccttcgggac gggtgtgggc ctcgagatcg gggaccgcga
    49861 cctccgggtc gtggtcgtgc gtattcgacc gtccagcgtg agcgttctcg gctcagcgac
    49921 gatctccgat ttccgcggtc gtccggccgc ggaatgggga gctgagtact tcgaattcgt
    49981 ccggggcgcc ggcgcgggtc atgtggcggc cttcgcactg cttccgcggc gcgaggtaat
    50041 cgtgcggcac gtcaatcttc ctggagtagc cgacagggac cttggcgccg ccatcgagta
    50101 ccagatcgat gccctccacc catacggcga gggcgaggcc cactattcct gggcccggct
    50161 gagcgacaag tccactgtgc ttgtcggcat cactcgccgg accgtaatcg cgcagtatca
    50221 cgaggtgctc ggcgaagccg gaatcaagct cgccgccctt acgttctccg ccgccacgct
    50281 gtattcagca gcgcgtttat tcggcgcgcc tcccgcggac ggctttcttg cgctggcggg
    50341 gcaggacggc gaaatggagt tgtacgggga gagcgcgtcc cggccggtct tctcggccgt
    50401 catgggtggg cctccggaga aagccgcgcc actggccgca gcggaactcc gtctcgctcc
    50461 cgacgccgcg ttactgtcgc tttcccagat ccttcccgcg ccgaaactgg cgcccgccga
    50521 tttcgacgtc gagtgcttcg cccttccgta cgcagcggcg ctggcggggg cgtctcccag
    50581 gctcgcgctt ccgatcaatc tgctgccgcc ggatctcagg agcaccagtt cccgggcgat
    50641 gttcgtgcct actgctgcgc tggcgacagt tctcgccggc ctgctggtgg ctctggcggc
    50701 tcaagcgcgt ctccaggagc ggcgccacct tgaggccgtt caggccgaga tcgcaaagtt
    50761 cgagcggcgt gcgtcgaggg tggctgcggc ggaaaccgaa accgggaaac tgcgggcgcg
    50821 aatccacctg cttgacgcat tccgccagcg cacggcctcg gatctcgacg ccatcaagga
    50881 gatcacggcc ctgctggcgc ctcccgcctg gctcagttcg ctcgagttga cacgcgattc
    50941 gctgagcctg gccggagaga ttgagcaggc cgctcctctt ctcaagactc tcgatggttc
    51001 gccgcgattc cgcggttcgg agttcactgt accgatcctg cgtaccgcaa aagtggaggt
    51061 gttccgcatt cgcgcttccc gggaggaggt cgcgccatga cgcttcagaa gcgcgacaag
    51121 cgggcgctgg tgattcttgg ggtggctgcg gctgcgctga tcgtctggaa ctacttgctg
    51181 acccgcgagt ccgctacccc tgtcgtctcc gccgccggca gcctcccggc ggccgaaaag
    51241 cggctcgtcc ggctgcgcca gacggctgcc ctgcttcccg cccgggagga gacgctgaaa
    51301 gcggtgaggg atcaactggc gttacgggag aagggcctga ttcaggctga gaccggacca
    51361 caggcgcaag cgcaattgct tcaaatcctt cgccgattga ccgccgctca gtccatcgag
    51421 gtccgaaccg tcgaaatggg gcaggtgaga acgctgggcg acgattacgg cgaggtgtcg
    51481 gtgccggtga cttttgactg ccggatcgag caactgctga atctgctcgc cgaccttact
    51541 tcgcagtccg aagctgtcgc cgcctccgag gtgcgcatcg gggtggcaaa tgaaaaagag
    51601 aagatgatca cggtgaggct gaccgtgtcc ggcgtcgtgc cgcgccgtct cgtgcctgag
    51661 aagaaaggag tggcacggtt ttgactctcc gcaagaagct gctcctgttg aacctcgtgc
    51721 tgctcgcgct gattgctgcg gtctcctggc gcttgcggca ggagtggctc gccgcccgtg
    51781 ctcgcgagca ggaggtgctc cggcaggcgt tgaagccggc tcccgcgccg cccatgtccc
    51841 cgctccccca accgaagtcg gtcaccgccg ccgcctacga cgatatcgcc cagaaggtgt
    51901 tgttctccaa ggaccggaat ccagttgtga ccatcgaagt ggcaccgcca aagccgctgc
    51961 ctcccttccc ggtttctcac ggggtcatga accttggcga cggcccgatg atcctcctta
    52021 gcgagcaacc aggcaaacct caccgtgcgt acacgccggg cgacaaggtc ggcgaataca
    52081 agctcgtggc cgcaaacaac gaggaaatcg ttctggagtt cgatggccgg caggtcaaga
    52141 agcggctcga cgaactgatc gaccggagtg cgaaggagga accgtccccg gctcccgggg
    52201 cggggcgacg gtcgaatgcc gcgcaacctg cggcgcaaca tccgagtccc gctgccggct
    52261 cccagagtgg tccaggcgcc gtgatgtccg gcgaagtgcg agcttgcttg ccgggtgata
    52321 cagcgccggc gggcacggtg aacaacgggc tgcgaaaagt gatcacggag tcgccctttg
    52381 gcaaggtttg ccgctgggaa ccggtgcgct aaagacgtga atacgcatag gacaaacgca
    52441 agatgaaacg gattctactg cttctgctgt cactcgctct gttcgccttc ggcgcggagg
    52501 agaagaagaa agctcctcga ccgaagccgg gacagaaagc tcaggaaatc gccttgcccg
    52561 ccggcgcgca ggaaattgct ccctacacat accggtacaa ggacgaccaa ggcaagacct
    52621 ggatctaccg ccgcacgccg ttcgggctcg caaaattcga agaaaagctg gacgacaaag
    52681 ccgacgagat cgcgcccgag aatatgaggg ctttcgaaga aggcgatagc atccgattcg
    52741 agcgccccac tccgtttggg acagctcgtt gggtgcgcaa gaagagcgaa ctgaatgacg
    52801 tggagaaggc cgcttgggag cgggagctga aacgctccgt caagaccgca gaccggaagc
    52861 aggagcagtc acaagcgaag tcgcaacaat gaggaagccg atgagattct ggatttcgac
    52921 cggaaccatg tgggtggtgc tggccgcggt ggcgctcgcg cagcagaagc agcccgagcc
    52981 tattcaagtg gttcctccgc cgggcttcgg cccgcgcgtc gcgccgcccg ccaggacggg
    53041 aacgccaaaa tccaagagaa ccaagccgca gcgggagcaa tcgaagcccg cggcggagtc
    53101 ggcgaaaccc gaagcacagc cgattaagcc agaaacacag ccggccaagg ccgaagccaa
    53161 gcccgaagcc ggccagcccg gtgccgcccc cgcgcagccc gcgccgcagg aagtcgcgcc
    53221 cgtaaagccg ggaccggtaa cactctcgcc gacgccttcc ggccgttttg tcttgaacct
    53281 cgaaaatgcc tcgcttattc aggtcattga gatcctgacc cgccgtctca agctgaacta
    53341 catcctcgac cctcgcgtga acgtgaaagg cggcggaatc accctgaata ccttcggtga
    53401 gctcaaagag gtcgacatcc gggctctcct cgacactatc ctgcgcatga atggcgccgc
    53461 gatggtacag gtcggggagt tgcaccggat tgtgccactc accgagatca cgcgcatgcc
    53521 gcttagcccg caggtcgatc cgaaatcgat tccggaagac gagcgagtgg tactcaatct
    53581 catctttctc aagtacgcca ctgtgaccga gatggcaaag atcctcgaga agttcctcgg
    53641 cgagggcgcc gtcatggtca cttacgaacc ggcaaacctg ctgctggtgc tcgacaacaa
    53701 ccgcaacatg cggcggacca tggaactggt cgcgttgttt gacagcgggg ccctcgccag
    53761 ccagagagtg cggcttttcg aagtgaaaaa caatcggccg tcggacctcg tgaaggacct
    53821 ggagacagtg cttcagggtg cgtccttgac aggcaagctc ggaggggtgc gtttcctcgc
    53881 gttggaccgg atcaatacga tcattgcggt ggctccgaac cccggtgttt tcgaagaagt
    53941 ggaaaagtgg ctcggcaagc ttgacgtggc cgtgaagact acagccggtg cgaccgacaa
    54001 ttacgtctat cgtgtgaaat atgggcgggc gccaatgctg gcgacagcca tcatgcacct
    54061 gtatttggga attccctttt ccggttttgg ttatggtggt gggatgtatg gtggcgggat
    54121 gtacggagga ggccgccggg gcggttacgg tggctacgga gggtttgacg gtggcggcta
    54181 cggaggctat ggtggcggct acggaggcta tggcggcggc tatggaggct atggcggcgg
    54241 ctatgggatg cccggcggct acggaggata cggtaggggc gggtacggag gatacgggcg
    54301 tggatacccc gggtcgggtt acggtggcgt ctcgccgtac atgatgtcgt ccggaggaat
    54361 tgtgccgcca ccggccgcca caggtaccgc cgggacagta ggagccacag cgggcgctca
    54421 gcaggcgctt ccctcgggcg cggccacaag cccggacatg actggttcgt atctgggttc
    54481 gtgggactac ggcctgccgg aaggcattcc gcggattgtt ccgaaccctc tcgacaactc
    54541 gttgctgatc caggccacac cgcagcaata cgagcagatc ctcaagctcc ttcgcgacct
    54601 cgacgtgccc ccacgccagg tcctgatcga agcgaaaatc tatgaagttt cgctgaccgg
    54661 ggcgttcgcg ggcggagtga aggcgtacct ccagcgcaaa ggcgccaccg gcacgggcgc
    54721 ggccaccttt ggcaggattc ttcaggggac cacttccggc gccgggctga ctcttaccgc
    54781 cggtatgctc gtcggcgaaa gccgggagtt gctcggcatc ctgacggccc aggagactca
    54841 aggtaagacc cggctgatct cggcgccgag cgtcatcgcc accgacagca tcccggcaag
    54901 cattaacgtc gggcaggaag tgccgacgct cgcatcacag gccgtcacgg gagcacaaca
    54961 ggccggcacc t
//
LOCUS       DATIFQ010000207         4422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_835328, whole genome shotgun sequence.
ACCESSION   DATIFQ010000207 DATIFQ010000000
VERSION     DATIFQ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835328"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VLE22_09715"
     CDS             <1..272
                     /locus_tag="VLE22_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14726.1"
                     /translation="LIGTGLLTNASGSSIGHALPAKRNGFPCIAVVEQVGLAWNDLPG
                     MLYQSDIEDNSAWKPRFGRASGSPSPGMINKQAGGRLPAVRVTHQ"
     gene            complement(615..1322)
                     /locus_tag="VLE22_09720"
     CDS             complement(615..1322)
                     /locus_tag="VLE22_09720"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HSB14727.1"
                     /translation="MKNLTLMLTLALAAVVVRASANNAMVYAVHGIPGTALGAPTTDL
                     PVDVAVNGACVAALNGLNFGEIRGPLPLPAPANYTVEIKPADSLTPCSQATLLSTVVP
                     VTPGINASIVAHLTAGGAPKLAVFVNDVTWTSPGQGRFILHHTAQAPAVDVRVYRGDG
                     NGRSPSVPVPGFANGAQVGAEFRPGEWQATLSVGGNVVFGPATLKLQPKTVQLVYAIG
                     TFPDSFTVITKTIPAMR"
     gene            complement(1363..1836)
                     /locus_tag="VLE22_09725"
     CDS             complement(1363..1836)
                     /locus_tag="VLE22_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HSB14728.1"
                     /translation="MRSRLNVLLAGALALALTPFASAADIVDTAIAGKFNTLVAAVKA
                     ADLVETLKGDGPFTVFAPTDEAFAKLPAGTLETLLKPENKSKLQSILTYHVVPGKVMS
                     HDVVKLNSAKTVQGGSIAITVSGGNVMVNNARVTKADIETSNGVIHVIDTVILPE"
     gene            1999..2592
                     /locus_tag="VLE22_09730"
     CDS             1999..2592
                     /locus_tag="VLE22_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HSB14729.1"
                     /translation="MASETITTGLKKYAIGNKLRRLRLRKSMGLLELSKHTGLSPGLL
                     SKLERDVMYPTLPTLLRIAMVFSVGLEYFFNPEPKPVVEIVRKKDRLRFPDSPDAAQA
                     AYYFESLDFPVPNRPLNSYFAEFEPIADEQARLHEHPGVELLYLLSGRLELRIGGNKD
                     ELAEGDCIYFDCSVPHGYRRSGSKRTTALVVTCGPSG"
     gene            2922..3461
                     /locus_tag="VLE22_09735"
     CDS             2922..3461
                     /locus_tag="VLE22_09735"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HSB14730.1"
                     /translation="MKALLVSISIGVLIGAASLAPAQQSPPATETVTIGGKTITINYS
                     SPRVRGREGQLFGKEGRIGKDRTYPVWRAGANAATALHTNATLEMGGLTVPSGDYTLF
                     VNLADPDNWELIVNKQTGQWGLKYEQSQDLGRVKMTMSKPPALVENLKYTLSDEGGNR
                     GKLTLEWENHRASVTFTVK"
     gene            complement(3489..3941)
                     /locus_tag="VLE22_09740"
     CDS             complement(3489..3941)
                     /locus_tag="VLE22_09740"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HSB14731.1"
                     /translation="MRSLFAVVLLSVSVFAAVLNLGKPLTVKEPMSVQKLMAAKEQYL
                     GKTVQVKGTVTDVCRRMGCWMELVESSTGVRVKIKVEDGVIVFPKDSVGKTAIAEGVF
                     SKLEMTREEAVAQAQHEAEANKRKFDPATIKGPVTFYQIQGTGAVVTE"
BASE COUNT          891 a         1321 c         1284 g          926 t
ORIGIN      
        1 atctcatcgg gactgggttg ctgacgaacg ctagtggatc cagcatcggt catgcgctac
       61 cggcgaagcg gaatggattt ccgtgcattg ccgtggtcga gcaggtggga ctggcgtgga
      121 atgacctgcc cggtatgttg taccagtctg acatagagga taactcggcc tggaagccac
      181 ggtttgggcg ggcttcaggg tcgccttcgc cgggcatgat aaataagcag gccggcgggc
      241 ggcttcctgc ggtgagagtc acacaccagt gagccgcctc gcatgtcaac cacctcttga
      301 cccttcttca ggcgcgagac cttgtcgggc gaaggtggat tttcgtgcgg tgccactgtc
      361 ccgagctagg catacgtcgg atgcagtatt cccgttttca gacttcctcc cacatattcg
      421 ccaacggccg ggctttctac cttacacata agtgggcccg ctggccatct cctggcagcg
      481 ggcccgtgag ggaagaagcg tggtcaggca tgaggcaagc actctcaacc tcggcctgtc
      541 agacgtttga gttagcctcc gaccagtcgt tcggctttgt tcagcctgct gggttcgcct
      601 tctcgtgtcg gagttcaacg cattgccgga atcgtctttg tgataaccgt gaacgaatcc
      661 gggaatgtgc cgatcgcata caccaactgc accgtcttcg gctgaagctt cagtgtcgcc
      721 gggccgaaaa caacgttacc cccgacagag agcgtagctt gccactcgcc tggccggaat
      781 tcggcgccaa cctgcgctcc gttggcgaaa cccggaactg gcacggacgg ggaccgtcca
      841 ttcccgtctc cacgatacac tcggacatca acggcgggcg cctgagcggt gtggtgcagg
      901 atgaagcgcc cttgtcccgg cgacgtccag gtgacatcgt tgacgaaaac cgcaagtttc
      961 ggtgcgccac cggcggtgag gtgggccacg atactcgcgt tgattcccgg ggtcaccggg
     1021 actaccgtgc tcagcagtgt cgcttgcgag caaggcgtca gcgagtccgc cggcttgatt
     1081 tcgaccgtgt aattggctgg agcaggcagc ggcagcggac cgcgaatctc gccaaagttc
     1141 agcccgttga gagccgcaac acaggcaccg ttcaccgcaa cgtcaaccgg gaggtcagta
     1201 gtcggcgcgc ccagtgcggt tcccggaatg ccgtgaacgg cgtaaaccat ggcgttgttc
     1261 gcagaagcac gaaccaccac cgccgccaag gctagagtca gcatcagggt caagtttttc
     1321 atcgtctctc tttttccttt cacgtctttc tttggatcag ccctattccg gcaggatcac
     1381 cgtgtcgatc acgtggatca cgccgttgct ggtctcaata tcggccttgg tcacccgtgc
     1441 gttgttcacc atcacgttgc cgcccgaaac cgtgattgcg atgctgccgc cttgcacggt
     1501 cttggcagaa ttcagcttca ccacgtcgtg ggacatcact ttgcccggca cgacatgata
     1561 ggtgagaatg ctctgcaact tggacttgtt ctccggctta agcagtgttt ccagcgttcc
     1621 agcgggcagt ttggcgaaag cttcgtcagt gggtgcgaat accgtgaacg gtccgtcacc
     1681 cttcaaggtt tctaccaggt ccgcagcctt aaccgccgcc accagggtgt tgaatttccc
     1741 ggcaatcgcc gtgtccacga tgtccgccgc ggaagcgaac ggagtcagcg ccagcgccag
     1801 tgcgcccgcg agcaatacat tcaaacgtga tctcatcctt ttttcccctc tttctttctt
     1861 tccgattggc cggtgtcccc gacccacaac ttgatcttaa ggaaaaagtg ttttactgta
     1921 aagaacagag cggcgatagc tttcctgacg agaaagaaca cgccggcaac aggcactgag
     1981 aggaaaacga acttcgctat ggcatcggaa accatcacta cgggtctcaa aaaatacgcg
     2041 atcggaaaca aactgcggcg gttgcggctg cgcaagagca tgggactgct agagctgagc
     2101 aagcacaccg ggctttctcc ggggctgctt tccaagctgg agcgggatgt gatgtatcct
     2161 acgcttccga cgctattgcg catagcgatg gtgttcagtg ttggcctgga gtattttttc
     2221 aatcccgagc cgaagcccgt cgtcgagatc gtccgcaaga aggaccggct gcgctttccg
     2281 gattcgccgg acgccgcgca agctgcctac tatttcgaga gcctggactt ccccgttccc
     2341 aaccgcccgc tgaattccta cttcgccgag ttcgagccga tcgccgacga gcaggcccgc
     2401 ctgcacgaac atccgggcgt cgaacttctg tacctcctct ccggcaggct ggaactgcgg
     2461 atcggcggga acaaagacga gctagcggaa ggggactgca tatatttcga ttgcagcgtg
     2521 ccgcacggtt accgcagatc cggctcgaaa cgcacgacgg cgctggtggt gacctgcggg
     2581 ccttcaggct gaagttggcg cgtgaaaccc tcagttttct ccaggagtgc agttcaggcg
     2641 taggcaggct ggacctcttg atgcagggtt tacagctcga gacgcccagc aagtcccagt
     2701 ggcggaaaag gtgaagcaag cgcccgtttg atcccgcgat cggggcgtcg aggatggatt
     2761 acagggagag ggttcccgag atcccgtcta tcccgtatcg attccatcac agggtttgat
     2821 gacagggttt gatggagtca tggtggatac cagtgggtgt atactgctcg tggcaggatg
     2881 ccaaagcgaa gaccttggtc aagcgaagga ggggacctga catgaaagcc cttttggttt
     2941 cgatctctat cggagtgctc atcggcgctg cgagtctcgc cccggcgcag caaagcccac
     3001 cggcgacgga aacggtaacc atcggcggca agacgattac gatcaattac tcctcaccga
     3061 gggtgcgagg ccgcgaggga caactcttcg gcaaggaggg gagaatcggg aaagatcgca
     3121 cttatcccgt ctggagggcg ggcgccaacg ccgctacggc ccttcacacg aatgccaccc
     3181 tcgagatggg cggcctgacg gttcccagtg gcgactacac tctgttcgtc aatctggccg
     3241 atcccgacaa ctgggaactc atcgtcaaca agcagacggg gcaatggggc ctgaaatacg
     3301 agcagtcgca ggacctcggc cgcgtcaaga tgaccatgag caagccgccg gctctggtcg
     3361 agaacttgaa atacacgctg agcgatgagg gcggcaaccg gggcaagctc accctggagt
     3421 gggagaacca ccgcgcttcg gtgactttca ctgtgaagtg atccgttgcc aggcaggcgg
     3481 tctgatcctt attccgtaac caccgcgcca gtgccctgga tctggtagaa cgtcaccgga
     3541 cctttgatcg tggcagggtc gaacttccgc ttgttcgcct cggcctcgtg ctgcgcctgg
     3601 gcgacggcct cttcgcgagt catttccagt ttgctaaaca caccttcggc gatggcggtc
     3661 ttcccgactg agtccttcgg gaacacgatc acaccatctt ccaccttgat cttgacccgc
     3721 acgcctgtcg acgattcgac gagttccatc cagcagccca tccgccggca cacatcggtg
     3781 acggtgcctt tgacctgaac ggtcttgcca aggtattgct ccttggcggc catcagcttc
     3841 tggacggaca tcggctcctt aacagtcagc ggcttgccca ggttcaacac tgcggcaaac
     3901 acggatactg aaagcagaac gactgcgaag agggatctca taatcgtagg gtaaccaaag
     3961 atgggttcgg gttgcatcgc ttcagtcgga ctacgctctg ctcgaaagtg atgccgcatt
     4021 cagccgattc cgcttaacag acgtggcagc cttcttcgct ccagaccccc ttggggctgg
     4081 atagcttccc aaagcgcccg ctttatcccg ctatcggatt ccgcgaccgc atcgaggcgg
     4141 cgccaacttt tccgcccgga acatatcttg gctctgctgg cgccaatgtc cggattgccg
     4201 acaatccgcc cgctagtccc ggtaaacagt acggcgttac aagtaagccg ctcacgcggc
     4261 ggacgctacg cgtgcactgc caatcagcct cgttctactt caggacgagc ttgctttggt
     4321 gcggcactcc ggctacgata cacctgatgc tcgccggttt atgtggttcg cagcagttac
     4381 ctgccaagac cgaccaagtc ccgttgtctc aatcagttgc aa
//
LOCUS       DATIFQ010000208        26682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1410299, whole genome shotgun sequence.
ACCESSION   DATIFQ010000208 DATIFQ010000000
VERSION     DATIFQ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 26682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26682
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410299"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="VLE22_09745"
     CDS             complement(<1..1381)
                     /locus_tag="VLE22_09745"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14732.1"
                     /translation="MFQYRGALDAYLEAKRLAEITGDTEMLATVSANLSSLYLQMEEP
                     NAGLAVSRETLDFLRKEGKSKYLPLLLAQSALLSARVGDTRSALRLYLDAVEAADRMG
                     DTASRALASNRFGYLLMKRGWLDSAERVLLEAFRLSKLYRLQELDYSYYILGLLKLEQ
                     GELPTAGRLLDEAVKRALRGSGTVPLWRVYHARGQARLRQGSLKEALADLESALEFAR
                     RWRAELPFSDSVWTNTTADLQNVYTAYVCSAARLYFRTGDERYARQAFTASEENRAAG
                     LRLLLSVSGNRRMPVEYWQTLAQLRASETELLRGGKTEIRRRIDELRFRLTEIETRSG
                     LEMPGTEVPGRVPFDRVQGAMANDEALLSFHLGETESYRWELTGESFHMKRLPSRRRL
                     TELVKQFSLAVRAGSETEAGETLYAALFGHVGRSAREKAHWKVVPEHELFKAPLSALV
                     TGRRGREPVY"
     gene            complement(2002..3084)
                     /locus_tag="VLE22_09750"
     CDS             complement(2002..3084)
                     /locus_tag="VLE22_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14733.1"
                     /translation="MKARSMTRREVLAGASAALAAHAESGPVRLPKKIRVGMIGFDGH
                     YSEILDPLPRLPDVDVVAIADSDAGKMASAARHPKLAAVRQYRDYGEMLERESLDLVA
                     VNNNNGERAAAVLACTDKKLNVIAEKPLAIERGDLEKVRKSVTRNGVKLSMLLPMRFS
                     PPYLALKRIVDSGEIGEVAQIGAQKSYKVAGAPEWRKRRATYGGTIPWVGIHMVDLML
                     WTSGRDFKEVASFQARIGFPEIGDAENSAASLFRMDNGGAATLRMDYLRPETAPSHGD
                     DRLRLAGTKGIAEYYNTGGVTLVSSGRKLEEIRELPAEGSVFVDFLESVYLGKTPALT
                     MAEIWRVNEVVLRAREAAEQGRVLHI"
     gene            3216..3806
                     /locus_tag="VLE22_09755"
     CDS             3216..3806
                     /locus_tag="VLE22_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14734.1"
                     /translation="MHYRRLASLLLGAWLCGSLFLVAVATRNFGAVDEMLSAPGPEAA
                     KQIQKIGKEQARMLLRHQASLQNLGVFENWERAQLALGFLLLLALFFGVESRPSALVF
                     TLIMSAAVVFQHWLLTPEIAALGGLIEFIPSTVPAIERTRFWNYHTAYSITELVKLGL
                     GLLLALGLLKRRRRSVRQLDSEIDTVDHADHGHVDR"
     gene            complement(3705..4022)
                     /locus_tag="VLE22_09760"
     CDS             complement(3705..4022)
                     /locus_tag="VLE22_09760"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HSB14735.1"
                     /translation="MLIARVIGEVVATQKHASHEGRKALLVQPLNLDGTDRGDAVVAL
                     DAVDAGVGDRVLLATEGYSAMTSVGRPMSPIDMAVVGVIDCIDLTVELPDAPPAPLQQ
                     PQS"
     gene            complement(4016..4297)
                     /locus_tag="VLE22_09765"
     CDS             complement(4016..4297)
                     /locus_tag="VLE22_09765"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HSB14736.1"
                     /translation="MYLGRVVGCVWSTAKHENLEGQRLLLVQPVTPELKPTGRQIVCT
                     DCTGAGAGELIYWCRGRESSFPFLPREVPTDNTIVAIIDELHVKRGGPC"
     gene            complement(4302..4580)
                     /locus_tag="VLE22_09770"
     CDS             complement(4302..4580)
                     /locus_tag="VLE22_09770"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HSB14737.1"
                     /translation="MILARVVGTVVATRKDPRLEGKKLLIVKPVSPEGKDEAGYLVAV
                     DTVSAGYRETVILVSGSSARMAEGCKDRPVDTAIVGIVDSLSLDKPAR"
     gene            complement(4655..4942)
                     /gene="eutM"
                     /locus_tag="VLE22_09775"
     CDS             complement(4655..4942)
                     /gene="eutM"
                     /locus_tag="VLE22_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HSB14738.1"
                     /translation="MGEALGMIETRGLVAMIEASDAMVKAAKVTLVGWEKIGSGYVTA
                     LVRGDVAAVRAATDAGAAAARRVGELVSVHVIPRPHASLEDVLPIGKSNAK"
     gene            complement(4980..5759)
                     /locus_tag="VLE22_09780"
     CDS             complement(4980..5759)
                     /locus_tag="VLE22_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HSB14739.1"
                     /translation="MQKTERELRQDIVDVGRLVYQKSWVAANDGNISIRMDRERILCT
                     PTGVSKGMMHPDDLIVVDMQGNKIVGRKERTSEIAMHLAVYNLRPDINSVVHAHPPVS
                     TGFAAAGKPLNLALLPEVVIGLGCVPLAAYGLPGTPELIEPMLPLIPKYDAIMMGNHG
                     AVCYGEDVYKAFFRMETVEHFARISLVAELLGGPRVLPRVEVQKLLESRERYGIKSRS
                     SGEPGCPVPAEEACGGGERFEVTREELIGLVDEVLRARGLA"
     gene            5845..6672
                     /locus_tag="VLE22_09785"
     CDS             5845..6672
                     /locus_tag="VLE22_09785"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSB14740.1"
                     /translation="MDKRRMCPHCRAFISSSDRICPYCEAEVGPRAADRRASGQIVGF
                     IPHAHFTTSMILLVNFGLFAATAVYSLRMGDGGWMGIDGRTLVYFGGKLREAILMGQW
                     WRLITAGFLHGGLLHILMNSWVLFDLGAQVEEVYGASRMMVFYIVATVAGFLASTFWS
                     PAISIGASAGLFGLIGAMIAVGVRYPTSLGAAIRGLYIRWAIYGLIIGLLPGLAIDNA
                     AHLGGLAGGFCAAYVAGLPAPFRTTGERIWKIAGWAALLVTIYCFVRWFLWFRLATS"
     gene            complement(6684..8564)
                     /locus_tag="VLE22_09790"
     CDS             complement(6684..8564)
                     /locus_tag="VLE22_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14741.1"
                     /translation="MRGWLAYTDITLRLTMRDRVALFFSYVFPLLFFFIFAEISDAEQ
                     GGVITQVVTMVLILAILGNGLFGAGVRAVQDRELNILRRFKVTPISPAPILAASMVTG
                     WLVFLPVLLVTLGLAVLLYGMPFPERWVSLFALVSIGVLAFRAIGLIVASVVNSAQES
                     AILNQLLYLPMLFLSGATFPITALPSWAQVAVQFVPASYLATGFQGIMIGHESLAQNW
                     RSAAALLAATAVALFLSMKLFRWEKEEKVARTGKLWVAVVLLPFLGLGAWDAWTRDNL
                     NKAKILHRDLRRSRTLLIRDARIVIGDGKVIESGAVLIRDGRIAEVFEGKAPDAKTLR
                     AEAVEASGKTIIPALIDVRVSLGLPGGVYEPASAYQPDKAVLRAVAAYLYCGVAAVRS
                     SADAGGVVDRVRKLVAEGQWLGAEVFKSDHAGTVAGPFRELTAPEEFARMAAHGEAYV
                     PMLSAAEAEAAILTGDAEMFERSLVLQVGPPELIEGTKRMMSRAKPPGSREKAATAQA
                     DLDRATENLRRAWRTGVILAAGSGSGNLLVFHGPAIHRELQLWVDAGIPPEVALEAAT
                     YNGARILTALGRIGAVRKGLEANLLVVDGNPLQDIAATERISLVVFKGERVFRSALFE
                     QE"
     gene            complement(8561..9568)
                     /locus_tag="VLE22_09795"
     CDS             complement(8561..9568)
                     /locus_tag="VLE22_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB14742.1"
                     /translation="MFPRSAALRSPCWLRSPRPGDRESRATTDEPVISVRELRKCYGA
                     LEAVKGIDFEVGRGEVFGLLGPNGAGKTTTVEILEGLRPRTAGEVSILGIDPETTIGR
                     LKDRIGVCLQATNLPEKIKVSEAMRLFASFYSRRTDGTRLLDRLQLRDKRDRYYSQLS
                     GGQKQRLAVALALINDPQLVFLDEPTAGLDPQARLEIHGLVEELRREKRTVLLTTHYI
                     EEAERLCDRVAIIDEGRIIALGTPREIQSRTLGRSIIEIQLERPVMGDDLPRIGGADH
                     PHLSEDGRLLTLAASHPARALVDLVKWLDQRGIEPADIHLKRPTLEDVFLELTGKSLR
                     E"
     gene            complement(9507..10043)
                     /locus_tag="VLE22_09800"
     CDS             complement(9507..10043)
                     /locus_tag="VLE22_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HSB14743.1"
                     /translation="MELKGKRVAVLVDNIYQDMEVWYPLYRLREAGAEVVSIGLKAGE
                     TYHGKYGYPIVCEKSYDETRASDFDGVVVPGGYAPDIIRRHPKALQFVKDVNAQGKLV
                     AAICHAGWVLCSADILRGRRVTCFFAIKDDVVNAGGIWEDSEVVVDKNLVTSRKPDDL
                     PAFCRASIAVLAAQSTAR"
     gene            10095..12524
                     /locus_tag="VLE22_09805"
     CDS             10095..12524
                     /locus_tag="VLE22_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HSB14744.1"
                     /translation="MTDFEKLGVFYLGRPYDLDAKKAKEGLLLYDSKDLVTHAVCFGM
                     TGSGKTGLCLSLLEEAAIDGIPAVVIDPKGDLCNLLLTFPELRPEDFAPWINLDDARN
                     KGASPDEYARRQAELWKNGLASWGQDGARIRRLRDAVDVAIYTPGSTAGLPVSILKSF
                     SAPPPEIVNDTELLRERVGATATALLGLLGIEADPIQSREHILLSSILSYAWKQGQDL
                     DLAALIQQVQAPPFQRVGVLDVEAFYPSKERFGLSMTLNNLLASPGFEAWLEGEALDI
                     QSMLYTAQGKPRISILSIAHLSDGERMFFVSLLLNQTLAWMRSQSGTTSLRALLYMDE
                     IFGYFPPVANPPSKQPLLTLLKQARAFGLGVVLATQNPVDLDYKGLSNAGTWFIGRLQ
                     TERDQQRVLDGLEGATSNAGASLDRDEIKRTLSRLGNRIFLMNNVHEDAPEVFETRWA
                     MSYLRGPLTRAQIKMLMDPRKSAAPVAAAVSSAAPPVSSPATAEARPVLPPDVPQYFV
                     PVRGARPAGSRLIYRPMLLGSAQVRFADPKANIDVLRDYVYFTLVREGAIPVNWQEAI
                     EAQVEPGDLEKEHTPDAGFAPLPVIAGKAKSYASWGKDFLTWLYGSQSLGIFRSAGLK
                     QVSVPGETERDFRARLGQTAREERDRAVAKLRERYAPKTGALEERIRRAEQAVAREQE
                     QAKTQKMQTAISIGATLLGAFLGRKAVSTSTMGRAATAARTASRTWKETQDVGRATET
                     VETLKQQLQDLEAQFAAETKELESVINPMTEALETVSVKPKKTNISVRLVALAWTPHW
                     QDSTGGLTQAY"
     gene            complement(12592..13590)
                     /locus_tag="VLE22_09810"
     CDS             complement(12592..13590)
                     /locus_tag="VLE22_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HSB14745.1"
                     /translation="MAIKTTVVGSYPTPHWLIGDTSRTTLRDAIMVVLKTQELAGIDL
                     LADGELNRFDPSHPETNGMIDYFVRQLEGIRTRLSVADIQRFRSMPALQYRADAAGLV
                     VGQIEEGTLNLVRDWEFVRPLTRHPLKFTCTGPHMLSKVLMDRHYGSPRGVCLGIARV
                     LKQQLQMINADVIQLDEANISGHPEDTEWALQGINMVLEGITGERAVHICFGNYGGQT
                     VQRGLWKDLLPYLNGLNANHVILEFARRGFDELEVLKDVDPKIGIGIGVVDIKDTSIE
                     SPDQIAKRIEHAASVLGVDRIRYVHPDCGFWMLQRSVADGKMHSLVAGRNLFAGKQ"
     gene            complement(13614..14432)
                     /locus_tag="VLE22_09815"
     CDS             complement(13614..14432)
                     /locus_tag="VLE22_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB14746.1"
                     /translation="MRRAMRMLGWLAFALIAYGLMWVMANRSIYFPMKYPSGWWEAQD
                     QIGASDIWLTTQDGVRIHGWWAPAGEGTVVTLYLHGNAGNLSHRAAHLREITMAGSSV
                     LIIDYRGYGRSDGRPTERGLYADAEAGYRHLLDAGYVPERIVLHGESLGTAVAVDLAS
                     RVKCGGVVLEAPFTSARAVAWKALPLIGPALVWGFDSKRKIGSVRAPVLVIHGEEDEV
                     IAFDFGKQLFDAANEPKFFRSIPGGGHNDIVEATGPKYREWLREFYEKALPGKG"
     gene            14523..15614
                     /locus_tag="VLE22_09820"
     CDS             14523..15614
                     /locus_tag="VLE22_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSB14747.1"
                     /translation="MKPLPSFRSHVITTEFKGGYQVVAADLNKDGRPDLIGLASGMNE
                     LVWFENPGWKRHVLAGGLSRLINVAASDNDGDGIPELVLATGFSMDAARSSGEVWMLR
                     HAGDPLVPWKILQIDRLPTSHRLRWADIEGNGQKVAINAPLSDARATAPDYHGKTPLV
                     FYRSGAWKREMIPIETDGVMHGVTTLDWDGDGREEILTASFEGIHLLRHAGPDRWTRT
                     ELAKGSPEPWPKSGASDIAAGRLEGCRFLCAIEPWHGNQVTVYIHLGERWDRRVIDDT
                     LVEGHALATADLDGDGRDEIVAGFRGKGHTISIYKAGDAEGSRWTRHIIDTEVPAASC
                     VVADLNGNGLPDIACIGGPVLKWYENLGR"
     gene            15657..16670
                     /locus_tag="VLE22_09825"
     CDS             15657..16670
                     /locus_tag="VLE22_09825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB14748.1"
                     /translation="MPVPQVTVVIPTLVADSALDDCLRALGEQTFRNFEVIVVDNSGN
                     GLARRSASIIAGTVVIDNPANVGFGAAINQGCIASRARYLATLNDDAVPHPGWLAALT
                     SALEARPDAGMAASRILLSGTGRLDSAGMLVCADGSSKQRGHGELPTRFPDPEEALFP
                     SACAALYRRSMLEDTEGFDEDFFLYSEDTDLGLRARWAGWKCLYVPGAVVEHRYSHSA
                     GRASPLKAYYVERNRLFVIAKSFPLPALCRAPFVAFARYFWHAWFLLRGRGTAARFRE
                     EGHGALRLAWYILSAHVALVVRARSLWKKRREIRDGARITTAEFLRLLRVHSISAREV
                     ASL"
     gene            16667..17398
                     /locus_tag="VLE22_09830"
     CDS             16667..17398
                     /locus_tag="VLE22_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB14749.1"
                     /translation="MTPALHDRDLLIIVPAYNEAASVGQVVHEVREAVPGVPVVVIDD
                     CSTDGTSTVAREAGASVLSLPHHLGLGGGVQAGYKLAYELGYEYVIRVDGDGQHDPRD
                     IPRIYEALRTNAYQMVIGSRFLENDADQTSFGRSVGVRFFRIVLRPILGKPVRDPTSG
                     FVGVNRQALLVFSRSFPLEYPEIEALVVLQRRAFQFAEIPVRMRPRLGGRSSITAVKS
                     IYYILHVLLGVFVNVLKFDRRRWKR"
     gene            17401..17769
                     /locus_tag="VLE22_09835"
     CDS             17401..17769
                     /locus_tag="VLE22_09835"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HSB14750.1"
                     /translation="MDRLLNFMTLLSVALIVLVLRSVRRAHIRVEYSVSWLGAAVTLL
                     VLSRSNWALDSIARLLGIADPPLALLLVVGCIFLIVLYRFSTVISDLKDANISLAQRV
                     GILEYHLRNLHENQENPKPR"
     gene            17738..19462
                     /locus_tag="VLE22_09840"
     CDS             17738..19462
                     /locus_tag="VLE22_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14751.1"
                     /translation="MKTRKTQSPAKASPGFSLKPWHVAVGVLAALFALFESYGPALNG
                     VFVFDDESLPFYSRDFGARPLIPAITGVRPFLMFTFWLNHQISGLEPYGYHLLNVLFH
                     FASGVLVFFICRKVLEWAAVAGGRRELLAAFAGCLFLFHPIQTESVAYIASRSENLSI
                     LFCYAAFALFLYRRSRAVSWPVVAGVLVLFAAAASTKEHTIVLPAVLLLTDYYWNPGF
                     SFEGACKNWRLYGTMAVVGAAGVYSVWNLLRTAPTAGFGVKDLPWNHYLYTQWRAMWV
                     YFRLLVFPAGQNADYRYPVSRTPLEPGTLIGLAVLLVAVGLAIRFRQRYPLASYGFLA
                     ALLLFAPTSSVIPIRDVVAERRVYLPMIGLLFVMLEFLRRWKLDLRRMAAVLSSILLV
                     CAVLTYQRNKVWAGPVPLWEDVLAKSPQNSRAHFQLALGYYEEGRCEESVRHYETAER
                     LGEREHRLYVDWALADDCLGRFDGALAKIKRAIEIEKTAHAYALMGMIYAKQNRRDEA
                     MTALDEAGRIDAGYDMTYAYRGNLLVLANDFAGAAAQYRRALELDPNNQLARNGLARV
                     QAALGAAR"
     gene            19499..20590
                     /locus_tag="VLE22_09845"
     CDS             19499..20590
                     /locus_tag="VLE22_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSB14752.1"
                     /translation="MVPGGVGGTEIYLRHLLAALAGIDSVNEYVIFTNRETGADLVPV
                     RPNFVHEPQSVRAASRPARLLWEQTVLPLAVRRARLDVLFNPGFTCPALCSCPAVTVF
                     HDLQHKRHPEHFRWFDLPFWRIFLYLAARRSKFLIAVSDATRKDLERFYHLATERIRV
                     VPEGVDERLFEIGRDRAAFEAESFLLAVSTLHPHKNLDRLIRVFSRFHTDRPDFRLVI
                     VGLRGFHAEEIEKEVQELGLGGAITLTGWIPRDQLYSLFRRAHSFVYPSTFEGFGLPV
                     LEALAAGIPSACSAIEPLMSLAGDAALQFDPQDEDAMLEAIRRLTCDLALRGQLAQQG
                     PRRAAAFSWKKTAEATLAALRDAVFNPRA"
     gene            complement(20609..21448)
                     /locus_tag="VLE22_09850"
     CDS             complement(20609..21448)
                     /locus_tag="VLE22_09850"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HSB14753.1"
                     /translation="MPATEIEALFDAKPEQYTEEHFRLFQRFKYALNAGEARAAEPDA
                     SAKSGWRVNGWVKKGILLGFRMGALVDMSVNTARQPYFDKATYPVKRLTVESGVRIVP
                     GGSSIRDGCYVGRGVTCMPPMYINVGSYVGDGTMIDSHALVGSCAQVGRNCHISAAAQ
                     IGGVLEPVGALPVIIEDEVLVGGNCGVYEGTVVKRRVVLGTGTILNRSTPVYDIVKGE
                     VYTATDDQPLIVPENAVVVAGARAIPHGPGKDWGISLYTPVIVKYRDAKTDAKIQLED
                     LLR"
     gene            complement(21435..22337)
                     /gene="dapA"
                     /locus_tag="VLE22_09855"
     CDS             complement(21435..22337)
                     /gene="dapA"
                     /locus_tag="VLE22_09855"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HSB14754.1"
                     /translation="MFKGCGTAMVTPFRKDLSLDEETLRRLVRRQIAAGINFLVPCGT
                     TGESPTLEHSEHLRVVEITLDEAKGKVPVLAGAGGYNTSEVISLAKQLESMGADGILS
                     VTPYYNKPTQEGLYQHYKAIAAAIRLPIIVYSVQGRTGVNVEPATLKRLAEIDNIFGV
                     KEASGNISQMSAILNLVPERFTVLSGDDAITLPLMALGGRGLISVASNEIPAEMTRLA
                     QLCLSGDFAAARAVHRKYLPLMDVNFVESNPIPVKAAMGLMGLLEPVWRLPLVPPSVV
                     NLAKIDKVLQDLALLEPKAIHAGN"
     gene            complement(22373..23065)
                     /locus_tag="VLE22_09860"
     CDS             complement(22373..23065)
                     /locus_tag="VLE22_09860"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HSB14755.1"
                     /translation="MNLAIVGYGKMGRLIEQLAPEYGFTVTLKLDEFNNANYEGITAD
                     NFRGVDVAIDFSIPPVVVSNVERISALGVNVVEGTTGWLGQLDHVKSVVEKAGTGMVW
                     SPNFSIGVNVFLRLVAEAARLLKDEPSYGAWGWEIHHSTKKDAPSGTLLKLFGEMKKA
                     GYGRPIDASSNRAGTHPGTHEIGFDSAADTITLRHSARNREGFARGALKAAQWVVGKK
                     GFFEFSEIMAGR"
     gene            complement(23067..24437)
                     /gene="lysC"
                     /locus_tag="VLE22_09865"
     CDS             complement(23067..24437)
                     /gene="lysC"
                     /locus_tag="VLE22_09865"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HSB14756.1"
                     /translation="MIVMKFGGTSVESAAAIERVASIVKARAERRPVVVVSAMGKTTN
                     KLLAIASDAVNGRRAQALDQLAELRAFHQREIDPILREGDRPEARRILDEHFGELAEL
                     SGGFPVLGELTPRSIDAISAFGERLSSALVTLAFRYFGMDAVHIDSRAVIVTDARHTQ
                     AAPLYPQSYERLAGKVAPLAQRKIVVMGGFIGSTEAGVTSTLGRGGSDFTASIVGAGI
                     SAEEIQIWTDVDGMLTADPTILPCGHRVKTISFAEAAELAYFGAKVLHPATVTPAIEK
                     NIPVLILNSRRPDVPGTRIVAEAVPCRNAVKSIACKRKITLVNIRSTRMLMAHGFLRR
                     IFESFDRYETPVDMVSTSEVSVSLTIDNPGRLPAILEELRQFAEVDTENNQAIVCLVG
                     ENIRYTPGVSGRVFHALEDVNVRMISQGASLLNLSFVVAEQDLAKTVDALHREFFAEV
                     DPAVFD"
     gene            complement(24437..25486)
                     /gene="asd"
                     /locus_tag="VLE22_09870"
     CDS             complement(24437..25486)
                     /gene="asd"
                     /locus_tag="VLE22_09870"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HSB14757.1"
                     /translation="MERKIQVGILGATGMVGQHFVKFLQNHPWFELTWLGASDRSAGK
                     KYKEACQWHLGGVTPETVADIIVDECKPGNAPKLLFSATDASVATEIERAFAQSGHVV
                     VSNSRNHRMEPDVPLLVPEINPDHLKVIPAQQHRWGWTGQIVTNPNCSTVVLTMGLAA
                     LKPFGITRVIATTLQAISGAGYPGVPSMDINANVIPFIGGEEEKMQVETQKILGYVNG
                     DAIHSLEAKVSAHCNRVPVVDGHTVTVSVELSAKPTEAELRQAFDTFTSEPQKRGLPS
                     APARPVIYMPEANRPQPRKDVERERGMAVFVGRLRECPVLDWKFVALGHNTVRGAAGA
                     AVLNAELMVSEGLLD"
     gene            complement(25486..26505)
                     /locus_tag="VLE22_09875"
     CDS             complement(25486..26505)
                     /locus_tag="VLE22_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSB14758.1"
                     /translation="MNLFELTRTLIDLESITGNERQVGIFLNDLLATLAAKHGGRAES
                     VEIEPDRFNVFACWGEPVVTLSTHMDTVPPHVPSREDGESIWGRGACDTKGIIASMIT
                     AAERLLADGVRNFGLLFVVGEERNSAGAMAAARMPRGSRYLINGEPTGNKLALGSKGA
                     LRYEISAQGRMAHSAYPELGESAIEKLLDVLQELREMPLPVDPVLGRSTMNIGTITGG
                     RAPNVIPDSAKAEVMIRLVSDPGPTREGVKKAADGRADAREVLCIPALQLGSLDGYDT
                     TVVSYTTDIPAFEGAWGTPFLIGPGTIHVAHTLDERVPKTELVEAVEIYQQMVRQLLA
                     KEKEA"
     regulatory      complement(26573..>26682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         4776 a         8923 c         7666 g         5317 t
ORIGIN      
        1 ggtacaccgg ctcgcgcccg cgacgccctg taacgagagc ggagagcgga gccttaaata
       61 gttcgtgctc gggcacgacc ttccagtgag ccttttcacg cgcggaccgg cccacgtgac
      121 cgaacaacgc cgcgtacagc gtttcgcctg cttcggtttc gctgccggcc cgcacggcca
      181 ggctgaactg tttcaccagc tccgttagcc gccggcggga gggcagtcgc ttcatgtgga
      241 aggactcccc cgtcagctcc cagcggtacg attcggtctc gccgaggtgg aaactcaaaa
      301 gtgcctcgtc gttggccatc gcgccctgta cgcgatcgaa cggtaccctg ccgggcacct
      361 cagtgcccgg catctcgagt cccgatcgcg tctcaatttc ggttagtcgg aatcgcagtt
      421 cgtcgatccg gcgccgtatc tccgtcttgc cgccccggag caactctgtt tccgacgccc
      481 ggagctgagc gagggtctgc caatactcaa caggcattct tcgattgccc gagacgctga
      541 gcaacaaccg caagccggcc gcccggttct cctcggaggc cgtgaaggcc tgccgcgcgt
      601 aacgctcgtc gccggtgcgg aaatagagac gcgcggccga gcatacgtac gctgtgtata
      661 cgttctggag atcggcagtg gtatttgtcc acactgagtc cgaaaacggg agttccgcgc
      721 gccaacgtcg tgcgaattcc agagcacttt caagatctgc gagggcttct ttgaggcttc
      781 cttgccggag ccgagcttgc ccacgcgcgt ggtacacacg ccacaacggc actgtgccgc
      841 tcccccgtaa tgctcttttg acagcttcgt cgagcagtct gccggcagtt ggaagttccc
      901 cttgctccag tttcagcaag ccaagaatgt agtaggagta gtcgagttcc tgaaggcgat
      961 atagtttgct caaccggaat gcctccagca acacccgttc cgcgctgtcc agccagcccc
     1021 gcttcatcag caggtaacca aaccggttcg aggccagcgc gcgactggcg gtatcaccca
     1081 tccggtcggc tgcctcgacc gcatccaggt aaaggcgcaa agcgctcctc gtgtcgccca
     1141 ctcgtgcgct gagcagcgcg ctctgtgcga gcaggagcgg gaggtatttg ctcttgccct
     1201 ctttgcgcaa gaaatccaga gtttcgcgtg acacggcgag cccagcattt ggctcctcca
     1261 tctggaggta cagcgaagac aaattcgccg agacggtcgc gagcatctcc gtatcccccg
     1321 ttatctccgc cagccgcttt gcctcaagat acgcatcgag cgcgccgcgg tattgaaaca
     1381 tcgcgaagcg gcaacctccg agattaatca ggaagcgaat ggctgacgcg gtatcgcgcg
     1441 accgcagcga cctccggtat ccctcagcat gaatgtgagc cgcgcggagg tactctcggt
     1501 tccggaatag ggcgtttccc tgagctcgga gtttctgcag cgcgggatcc tgtcggtggc
     1561 cacgccgagg tcgaataagt gggccggccg tcatcatcag catgaggacg agtgcggcgg
     1621 ttgagagtgt cttcatgtgt gagagaggaa aagggaatgg aactatgagc ctgaagttcc
     1681 taggagtacc ctaaccagga gtactcctag gaatcatacc agacattgcc gtggatatgg
     1741 caaccatgtc taatggttaa aacttagacg tgcgcctgtg gtcgcgcacg agagggttct
     1801 gaacttccag gggccacagc cgatcagtcg acaatgatga tgatcggggg cattcggtac
     1861 ctcgatggga gtgtgctcta ctcccgatcc gaggatagcc acagagcggt gtttcccggc
     1921 ctgaactgaa actcataagg tgtgtatttt cattgaatta caacattcag ttgtcttgtg
     1981 actggcaaat acccgggtcc tctagatgtg gagaacccga ccctgctcag ccgcttcgcg
     2041 tgcccgcaag acgacttcat tgacacgcca gatctcggcc atggtaaggg cgggtgtctt
     2101 acctaggtat accgactcaa ggaagtcaac gaacacggaa ccttcggccg gcagttcgcg
     2161 gatctcctcg agttttcgcc ctgaggaaac gagcgtgacg ccaccagtgt tatagtactc
     2221 ggctatccct ttcgtcccgg cgagtcgcaa ccggtcgtca ccgtgggagg gggccgtttc
     2281 agggcggagg tagtccatcc gcagcgtggc tgcgccgccg ttgtccatcc ggaacaggct
     2341 ggcggcactg ttttccgcgt ctccgatctc cggaaaaccg atccgcgcct ggaacgaggc
     2401 gacctccttg aagtcccgcc cgctcgtcca gagcatcaga tccaccatgt gaatgcccac
     2461 ccatggaatt gttccgccat aagtggcgcg tcgcttgcgc cattctggcg cgccggcgac
     2521 cttatacgac ttctgggcgc cgatttgcgc cacttctccg atctcaccgg aatccacgat
     2581 gcgtttcagc gccaggtatg ggggagagaa ccgcatcggc agcaacatgg acaatttcac
     2641 accgtttcgc gtaacggact tcctcacttt ctcaagatct ccgcgttcga ttgccagcgg
     2701 tttttccgcg atgacattca gtttcttgtc cgtgcaagcg agaacggcgg cggcacgctc
     2761 accgttgttg ttgttgaccg caacgaggtc cagtgattcg cgttcgagca tctctccgta
     2821 atcccggtat tgccggacgg cagccagctt cggatgccgg gcggcgctcg ccatcttgcc
     2881 ggcatcggaa tcggcgatgg cgaccacatc aacgtcgggc aaccggggca gcggatcgag
     2941 gatctcgctg tagtggccgt cgaaaccgat catgcccacc cgaatcttct tcggcagcct
     3001 aaccggaccg gattccgcat gcgctgcaag cgcggcggac gcgccggcca gcacctcgcg
     3061 gcgcgtcatc gagcgtgctt tcattggtga catgccgaga ttgtcccata caatcgcgtg
     3121 atgcgctccc accggcttcg cccagcgagg cgaaggctta ccggctaaga ttcgagcgcc
     3181 tgtcgccttg cggtagactg gggaattcct tcggtatgca ctatcgtcgg cttgcttccc
     3241 tacttctggg cgcctggctt tgcgggagcc tgtttctggt ggccgtagcg acgcggaatt
     3301 tcggcgctgt cgacgagatg ctcagtgcac ccggaccaga agccgccaaa caaatccaga
     3361 aaatcggcaa ggagcaggcg cggatgctcc tccgccatca agcctcgctc caaaaccttg
     3421 gggtgtttga gaactgggag cgcgcgcagc ttgcgttggg cttcctgctg ctgctggctc
     3481 tcttctttgg agtggagagc cggccgtcag cactggtctt cacgttgatt atgagtgccg
     3541 cggtggtatt tcagcactgg ttgctgacac cggaaatcgc ggcgctgggc ggcttgatcg
     3601 aattcatccc atcgacggtt cctgcgattg aacgcacgcg cttctggaat tatcacaccg
     3661 cctactcaat caccgaactg gtgaagctag ggctcggact cttgttagct ctggggttgc
     3721 tgaagcggcg ccggcggagc gtcaggcaac tcgacagtga gatcgataca gtcgatcacg
     3781 ccgaccacgg ccatgtcgat cggtgacatc ggccggccga cgctggtcat cgcgctgtag
     3841 ccctcggttg ccaggagcac ccggtcgccg acgcccgcgt ccaccgcatc gagcgccacg
     3901 accgcatcgc cccgatctgt tccatcgagg tttaggggct gcaccagcag cgccttccgc
     3961 ccttcgtggc tcgcgtgttt ctgcgtggcc actacctcgc cgatcacacg agcgatcagc
     4021 atggcccgcc tcgcttcacg tgaagctcat cgatgatggc aacgatggtg ttatcggtgg
     4081 ggacttcccg cggcagaaag gggaaactcg attcccttcc ccggcaccaa tagatcaact
     4141 ccccggcgcc ggctccggtg cagtccgtgc aaacgatctg tctgcctgtt ggctttaatt
     4201 ccggcgtcac cggctggacc agcagcaagc gctgcccctc aaggttttcg tgtttcgcgg
     4261 tggaccagac gcagcccaca acccgtccca gatacatggc ttcaccgtgc gggcttatcg
     4321 aggctcagac tgtcgacgat gccaacaatc gccgtatcca ccgggcggtc cttgcagccc
     4381 tccgccatgc gcgccgaact gccggagacg aggatcaccg tctcccggta tccggcgctg
     4441 acggtgtcga cagccaccag gtagccggcc tcgtccttcc cttcgggaga aacgggcttg
     4501 acgatcagca gcttcttgcc ttccaggcgg gggtccttcc gtgtagcgac cacggtgccc
     4561 actacgcggg ccaaaatcat tggtatgcct caccgcttgt cgtgcgtggc ccgcggctcg
     4621 tgatgtccac aagccgccag ccgcgtgcga caggctactt cgcgttcgac ttgccgatcg
     4681 gcagaacgtc ctcaaggctc gcgtgcggcc gcgggattac gtgcacagaa accagctcgc
     4741 ctacccggcg cgcggcggca gcacccgcgt cggttgccgc ccgaacggcg gcgacatcgc
     4801 cccgcaccaa cgccgtcacg tagccggatc cgatcttttc ccaaccgaca agcgtcacct
     4861 tcgccgcttt caccatcgcg tcggaggcct cgatcatggc caccaaaccg cgagtttcga
     4921 tcattcctaa cgcttcaccc attcggcctc caggtacaac cggaaatcaa gaacttgttt
     4981 catgcaagcc cgcgggcccg cagcacttcg tccaccagcc cgatcagctc ttcgcgggtc
     5041 acctcgaagc gctcccctcc gccgcaggct tcctccgccg gcacaggaca gccaggctca
     5101 ccgctcgagc gcgacttgat gccataacgt tcccgcgact caaggagttt ctgcacctcg
     5161 acccgcggca acactcttgg accgcccagc agttcagcca ccagcgagat acgcgcgaag
     5221 tgctccactg tttccatccg gaagaacgcc ttgtagacgt cctccccgta gcacaccgcg
     5281 ccgtggtttc ccatcatgat cgcgtcgtac tttggaatga gcggtagcat cggctcgatc
     5341 agctcgggtg tgcccggcaa tccgtacgca gccagcggga cgcaacctaa accgatgacg
     5401 acctccggca ggagggccag gttcagcggc ttgcccgccg ccgcgaatcc cgtagagacg
     5461 ggcgggtgtg cgtgaaccac cgagttgatg tccggacgga gattgtacac cgccagatgc
     5521 attgcgatct cggaagtccg ttctttgcgg cctacgatct tattgccctg catgtcgacc
     5581 acgatcaggt cgtccgggtg catcattccc ttgctcacgc ccgtcggcgt gcagagaatc
     5641 cgctcccggt ccatccgaat gctgatattg ccgtcgttgg ccgcaaccca gcttttctgg
     5701 tagacgaggc ggccgacatc gacgatgtcc tgccgcaact cccgctcggt cttttgcatc
     5761 cgaatccaag attgtagcag acggccgtcc gtcgccgttc cgttgtgaca gcgccggcga
     5821 agcgcgaatg tacaatggtt cgtagtggac aaacgtcgca tgtgcccgca ctgccgggcc
     5881 ttcatctcct cgagcgatcg catctgcccc tactgtgaag cggaagtcgg acctcgtgcc
     5941 gccgaccgca gggcatccgg ccaaattgtg ggcttcatac cccacgccca cttcaccaca
     6001 tcgatgatcc tgctggtgaa ctttggcctt tttgccgcca cggccgtcta cagccttcgc
     6061 atgggcgacg gcggatggat gggcattgac ggccgcacgc tggtctattt cggaggcaag
     6121 ctgcgcgagg cgatactgat gggccagtgg tggcgcctca tcactgccgg tttcctccac
     6181 ggaggactgc tgcatatcct gatgaactcc tgggtcctgt ttgacctcgg cgctcaagtc
     6241 gaggaggtat acggcgccag ccgcatgatg gtgttctaca tcgtcgccac cgtcgccggc
     6301 ttcctcgcga gcacattctg gtccccggcg atctccatcg gcgcatccgc cggattgttc
     6361 ggccttatcg gcgctatgat cgccgtcggg gtccgctatc ccacttcgct cggagccgcc
     6421 attcgcggcc tttacatccg ctgggccatc tacggtctga ttatcggctt gcttccggga
     6481 ttggctatcg ataacgccgc acacctcggc ggtctggcgg gcggcttctg tgccgcttat
     6541 gttgccggtt tgcccgcccc cttcaggacg accggcgaac gcatctggaa gatcgcgggt
     6601 tgggccgctc tcctcgtcac gatctactgt ttcgtgcgat ggttcctctg gttccgtctc
     6661 gcaacgtcct gagccggtca ccgttactcc tgctcgaaga gcgcggaccg aaagacccgc
     6721 tcccctttga acaccactag cgagatccgt tctgttgccg cgatgtcctg aaggggattg
     6781 ccgtccacca ccagcaggtt cgcttccaac cccttccgca cggcgccgat tcgacccaaa
     6841 gcggtcagaa tgcgcgcccc gttatatgtc gccgcctcca gcgcaacttc cggcggtatc
     6901 cccgcatcca cccaaagctg aagttcgcga tggatggctg ggccatgaaa caccagcaga
     6961 tttcccgagc cgcttccggc cgcgagtatt actcccgtgc gccacgcccg gcgcaggttc
     7021 tccgtagctc ggtcgagatc cgcttgcgca gtagccgctt tttcacgaga tcccggcggc
     7081 ttcgcccgac tcatcatgcg cttcgtaccc tcgatcagtt cgggtggacc cacctgaagc
     7141 accaacgagc gctcaaacat ttccgcgtct cccgtcagga tcgcggcctc cgcctctgct
     7201 gcgctcagca tgggtacgta cgcctctccg tgcgccgcca ttcgcgcgaa ctcctccggc
     7261 gcagtcaact cccgaaacgg tccagccacg gtaccggcgt ggtcgctctt gaacacctcc
     7321 gcgccgagcc actgtccttc tgcaacgagt ttccgcacgc gatcgacgac accgccagcg
     7381 tcggcagaac tgcgcaccgc ggcgactccg cagtagaggt acgcagccac ggccctcaac
     7441 acggctttgt ccggctggta cgcactggca ggctcataca cgccgcctgg aaggccaagc
     7501 gagacgcgca catcgatcaa ggctgggatg atcgttttgc cggaggcctc cactgcttcg
     7561 gcgcgcagcg ttttggcgtc cggagctttg ccctcgaaga cctcggcgat cctgccgtcc
     7621 cgaatcagca ccgcgccgga ctcgatcacc ttgccgtcac cgatgacgat ccgggcgtcg
     7681 cggatcagca gcgtccggct gcgccgcagg tccctgtgca ggatcttcgc cttgttcagg
     7741 ttgtcgcgcg tccaggcgtc ccaggcgcca agtcccagaa aagggagaag cacgactgcc
     7801 acccagagct tgcctgtcct cgcgactttt tcctccttct cccaccggaa cagcttcatg
     7861 gaaagaaaca gcgccaccgc tgtcgcagcg agcagagctg ccgccgatct ccagttctgg
     7921 gcaaggcttt cgtggccgat catgatcccc tggaagcctg tcgccagata cgatgccggc
     7981 acgaactgca ctgccacttg cgcccaggaa ggtagtgccg tgatcgggaa cgtagcccca
     8041 ctcaggaaaa gcatcggcag gtagagaagc tggttgagaa ttgcgctttc ctgagccgag
     8101 ttcacaaccg acgccacaat cagcccaatc gcgcggaatg ccagcacacc gatactcacc
     8161 agagcaaaca gcgacaccca ccgttccgga aatggcatcc catacagaag cacggccagt
     8221 ccaagcgtca ccagcagcac cggcaggaac accagccagc ccgtcaccat cgacgccgcc
     8281 aggatcgggg ccggcgagat cggcgtaacc ttgaaccggc gcaggatgtt cagctcgcgg
     8341 tcctgcaccg cccgcacgcc cgctccgaac agcccattcc ccaggatcgc aaggatgagc
     8401 accatggtca caacctgagt gatgacgcct ccctgctcgg cgtctgagat ctccgcgaag
     8461 atgaagaaga atagcagcgg aaagacgtag ctgaagaaca gcgccacgcg atcccgcatc
     8521 gtcagacgca gggtgatgtc cgtgtatgcg agccagcctc tcattcccgc aggctctttc
     8581 cggtcagctc gagaaaaacg tcttctagcg tcggccgctt gagatggatg tccgccggct
     8641 cgatgccccg ctgatccagc cacttgacca aatcgaccag cgcccgcgcc gggtgtgacg
     8701 ccgccagcgt cagcaagcgg ccgtcctcgc tgaggtgcgg gtggtccgcg cccccgatcc
     8761 gcgggagatc gtcccccatg accggccgct ccaactggat ctcgatgatc gagcggccca
     8821 gcgtgcgtga ctggatctcg cgcggcgttc caagcgcgat gatccggccc tcatcgatga
     8881 tcgctacacg gtcgcaaagc cgctcggctt cttcaatgta atgggtggtc agcaggaccg
     8941 ttcgcttctc ccggcggagt tcctcgacaa gcccgtgaat ctccagccga gcctgagggt
     9001 cgagcccggc ggtcggttcg tccaggaaca cgagctgcgg atcgttgatg agcgccagcg
     9061 ccaccgccag acgctgcttc tggcctcccg ataactgaga gtagtaccgg tcgcgcttgt
     9121 ccctgagttg aagccggtcg agcagcctgg tcccgtccgt acgtcgcgaa tagaacgagg
     9181 cgaacagccg catcgcttcg gaaaccttga tcttttcggg cagattggtg gcctgaaggc
     9241 agaccccgat gcggtccttc aggcggccga tcgtagtctc cgggtcgatg ccgagaatcg
     9301 agacctcgcc tgccgtcctg ggcctcagtc cttcgaggat ctcaaccgtc gtggttttcc
     9361 cagcgccgtt cggaccgaga agtccgaaca cttcaccccg tccgacttcg aagtcgatcc
     9421 ccttgacggc ttcgagcgcg ccgtagcact tacggagttc gcgaacggaa atgaccggtt
     9481 cgtcagtcgt ggcgcggctc tccctgtcac cgggccgtgg actgcgcagc cagcacggcg
     9541 atcgaagcgc ggcagaacgc gggaagatcg tccggcttgc gggaagtcac taggttcttg
     9601 tccaccacta cctccgagtc ctcccaaatg ccaccggcat tcactacatc atccttaatg
     9661 gcgaagaagc aggtgactcg tcgaccccgc aaaatgtctg ccgagcagag tacccacccc
     9721 gcatggcaga ttgccgccac gagctttccc tgggcgttca cgtctttcac gaactggagc
     9781 gccttcggat gtcgccgtat gatgtccggc gcatatccgc ccggaaccac cacgccgtcg
     9841 aagtccgacg cccgtgtctc gtcatacgac ttctcgcaga cgatcggata gccgtacttg
     9901 ccgtggtatg tctcgcctgc tttcagcccg atggaaacca cttcagctcc cgcctctcgc
     9961 aggcggtaga gcgggtacca cacttccatg tcctgataga tgttatcgac caggaccgca
    10021 actcgctttc ccttgagctc catatcccgc tattgtaccg tccacccccg cgctatcatc
    10081 gttggattgc tgccatgacc gacttcgaaa aactcggcgt cttctacctc ggccggccct
    10141 acgacctgga tgcgaaaaag gcaaaggaag gcctgttgct gtacgattcc aaggacctgg
    10201 ttacgcacgc cgtctgtttc ggcatgaccg gtagcggaaa gaccggccta tgcctgtcgc
    10261 tgctggagga ggctgccatt gacggcattc cggcggtcgt catcgatcca aagggagacc
    10321 tctgcaacct gctgctgact tttcccgaac tgcgccccga ggatttcgca ccgtggatca
    10381 atctcgatga cgcgcgcaac aagggtgcct cgccggacga gtacgcccgg cgccaggccg
    10441 agctatggaa gaacggcctc gcatcctggg gacaggacgg agcgcgcatc cgccgcctcc
    10501 gtgacgcggt ggacgtcgcc atctacactc cgggcagtac ggccgggctc ccggtatcga
    10561 ttctcaagtc gttctccgct cccccaccag agatcgtgaa cgacaccgag ttgcttcgtg
    10621 agcgtgtcgg cgctactgcc accgccctac tcggccttct tggcattgaa gccgatccta
    10681 tccagagccg ggagcacatt cttctttcga gcatcctaag ctacgcatgg aagcagggcc
    10741 aggacctcga cctcgcggcg ctcatccagc aggtgcaggc gccgccgttc cagcgtgtgg
    10801 gcgttctcga cgtcgaggcg ttctatccgt cgaaggaacg gtttggtctc tcgatgacgc
    10861 ttaacaacct gctcgcctcg cccgggttcg aggcttggct cgaaggtgag gcgctcgata
    10921 tccagagcat gctctacacc gcgcagggta agcctcgcat ctcgatctta tccatcgccc
    10981 acctgtcaga cggcgagcgg atgttcttcg tctcgctcct gctcaaccaa acgctcgcct
    11041 ggatgcgctc ccagtccggc accaccagtc tccgcgcgct gctttacatg gatgagattt
    11101 tcggctactt ccctccggtc gccaacccgc cgtcgaagca gccgctgctc accttactca
    11161 agcaggcgcg cgccttcggc ctgggtgtcg tgctggccac acagaatccg gtggatctcg
    11221 actacaaagg cctgtctaac gccggcacgt ggttcatcgg ccgcctccag accgagcgcg
    11281 atcagcagcg tgtcctcgac ggcctggaag gagcgacatc aaatgccggc gccagtctcg
    11341 atcgtgacga gataaagcga actctgtcga ggctcggcaa ccgcattttc ctgatgaaca
    11401 acgttcacga ggacgcgccg gaggtgttcg agacgcgatg ggcaatgtcg tatctccgtg
    11461 gcccgctcac tcgcgcccag atcaagatgt tgatggaccc acggaagtcc gccgcgcccg
    11521 tggcagccgc tgtttcttcc gccgcgccgc ccgtctcatc acccgcgacg gcagaggcgc
    11581 gccccgttct accgccagac gtgccgcagt acttcgtgcc tgtccggggc gcacggcccg
    11641 ccggtagccg gctcatctac cgaccaatgc tcctcggctc cgctcaggta cgattcgcgg
    11701 atcccaaggc gaacattgac gtccttcggg actacgtgta cttcacactc gtccgtgagg
    11761 gcgcgatccc ggtcaactgg caagaggcaa tcgaggcgca ggtggagccg ggggacctgg
    11821 agaaggagca tacacccgat gcggggttcg caccgctacc cgtcatcgct ggaaaggcga
    11881 aaagctacgc ctcatggggc aaggacttcc ttacatggct ttacggaagc cagtctctcg
    11941 gtattttccg gagcgcgggc ctaaagcagg tctccgtgcc cggggaaacc gagcgcgatt
    12001 tccgcgcccg cctcgggcag accgcccgtg aggagcgaga ccgggccgtt gcgaagctcc
    12061 gtgaaagata cgcgcccaag accggagccc tcgaagagag aattcgccgc gcggaacagg
    12121 ccgtcgcccg tgaacaggag caggcgaaga cacagaaaat gcagacggcg atctcgatag
    12181 gcgccacgct cctgggcgcc ttcctcggcc ggaaagccgt cagcacctcg acaatgggcc
    12241 gcgccgctac tgcggcccgt accgccagcc gtacctggaa ggaaacacaa gatgtcggcc
    12301 gggcgacgga aaccgtcgag acgctcaaac agcagcttca ggatctggaa gcgcagttcg
    12361 ccgcggagac caaggaactc gagtccgtca tcaacccgat gaccgaagct ctcgagaccg
    12421 tttccgtgaa gccaaagaag acgaacattt cggtacgcct cgtagctctc gcctggacgc
    12481 ctcactggca ggactcaacc ggcggactga cgcaggcata ctgaagactc agcctttact
    12541 tcagccgcct ccgccactcc cgccaggagt tgccgcacgg cccatggaac gtcactgctt
    12601 ccctgcgaac aggtttcgtc ccgccacaag tgagtgcatc ttgccgtccg ccacgctccg
    12661 ttggagcatc caaaaaccgc agtctggatg cacgtagcga atccgatcga cacctagcac
    12721 gctcgccgcg tgctcgatcc gcttcgcgat ctgatccggc gattcgatcg aggtgtcctt
    12781 gatatccaca acgccgatgc cgatgccaat cttcggatcg acgtccttca gcacctccaa
    12841 ctcgtcgaac ccgcgccggg caaactcgag gatgacatga ttcgcgttga gcccgttcag
    12901 gtacggcagc agatccttcc ataagcccct ctgaacggtc tggccgccgt agttgccgaa
    12961 gcagatgtgc accgcccgct cgccggtgat cccttcgagc accatgttga tgccctggag
    13021 cgcccattcg gtgtcttcgg gatgaccgct gatgttggct tcatcgagtt gaatcacgtc
    13081 ggcattgatc atctggagct gctgtttcaa caccctggcg atgccaaggc agacgccgcg
    13141 cgggcttccg tagtgccggt ccatcaggac cttgctgagc atgtgcgggc ccgtgcaggt
    13201 gaacttcaat ggatgccgcg tcagcgggcg cacaaactcc cagtcgcgca cgaggttcag
    13261 cgttccttct tcgatctgcc cgacgacgag tccggcggca tcggcacggt attgcagtgc
    13321 gggcatcgac cggaagcgct ggatatctgc tacgctcaac cgtgtccgaa tgccttccaa
    13381 ctgccgtacg aagtagtcga tcattccgtt cgtctcggga tggctgggat cgaagcggtt
    13441 cagttcgccg tccgcgagca ggtctatacc ggccagttcc tgcgtcttga gaaccaccat
    13501 gatcgcgtcc cgcaatgtcg tccggcttgt atccccgatc agccagtgcg gcgtcggata
    13561 gctccccaca accgtcgtct taatcgccat agcctttgta ttctatactt ggcctacccc
    13621 ttcccgggca aagctttttc atagaactcc cgcagccact cccgatattt cggaccggtt
    13681 gcctccacga tgtcgttgtg gccgccgccg ggtatcgaac ggaagaactt cggctcgttt
    13741 gccgcgtcga acaactgctt cccgaaatcg aatgcaatca cttcgtcctc ttcgccatgt
    13801 atgactagaa ccggggcccg gacactcccg atcttccgct tcgagtcgaa gccccagacc
    13861 agcgccggtc cgatcagcgg cagggctttc cacgccaccg cccgggctga cgtgaacggc
    13921 gcctccaaca caaccccgcc acacttcacg cgcgacgcga ggtcaactgc caccgccgtc
    13981 cccagcgatt ccccgtgcag cacgatccgc tctggaacgt acccggcgtc gagaaggtgc
    14041 cgatacccgg cctcggcatc tgcatagagc cctctttcgg tgggccgccc atcgctacgt
    14101 ccgtaaccgc gatagtcgat gatcaacacc gaagacccgg ccatcgtgat ctcgcgcagg
    14161 tgtgcagcac ggtgagaaag gttccccgcg ttgccgtgca gatatagagt cacgaccgtg
    14221 ccttcgccgg caggcgccca ccatccgtga atgcgcactc cgtcctgcgt cgtcagccat
    14281 atgtcgctcg ctccgatttg atcctgtgcc tcccaccacc cgctcgggta cttcatcgga
    14341 aagtagatgc tccggttcgc cattacccac atgagcccgt aagcaatcag ggcaaacgcc
    14401 agccagccga gcatccgcat cgcgcgcctc atacctcccc gccactctac cacccgtcgg
    14461 agctcgctct gctatcctgc cccgtgtatc ttctgatcgc gcttcttctt tccggagccg
    14521 ctgtgaaacc ccttccctct ttccgttcac acgtcatcac taccgagttc aaaggcggct
    14581 accaggtcgt cgcggcggac ctcaacaaag acggccggcc cgacctgatc ggccttgcca
    14641 gcggcatgaa cgagcttgtt tggttcgaga accctggatg gaaacgccat gtccttgccg
    14701 gcggcctctc gcgcctgatc aacgtggccg cctccgacaa cgacggcgac ggcatccccg
    14761 agctcgtact cgccaccggt ttctcgatgg acgccgcaag gagctcgggc gaagtatgga
    14821 tgctccgcca cgccggtgat ccactcgttc cgtggaaaat cctccaaatc gaccgcctgc
    14881 ccacctcgca tcgccttcgt tgggcggaca tcgaaggtaa cggacaaaag gtcgcgatta
    14941 acgccccgct cagcgatgcg cgcgccacgg cgccggatta ccacggaaag acaccgctgg
    15001 tgttctaccg ctccggagcg tggaagcgcg agatgatacc gatcgaaacc gacggtgtca
    15061 tgcatggcgt taccaccctt gactgggacg gcgacggccg cgaggagatc ctcaccgcca
    15121 gcttcgaagg gatccacctg ctccgccacg ccggaccaga ccgttggact cgcaccgagc
    15181 ttgccaaagg cagccccgaa ccctggccaa agagcggcgc cagtgacatc gccgccggcc
    15241 ggcttgaggg ttgccgtttt ctctgcgcca tcgagccctg gcacggcaat caggtcaccg
    15301 tctacatcca tcttggggag cgctgggatc gccgcgtaat agacgacacc ctcgtggagg
    15361 gccacgcact tgccaccgct gacctcgacg gcgacggccg tgatgagatt gttgccggct
    15421 tccgcggcaa aggtcacacc atctcgatct acaaagccgg cgatgccgaa ggatcccgct
    15481 ggacccgcca catcattgac accgaggttc ccgccgcttc ctgcgttgtc gccgacctga
    15541 acggcaacgg gctgcccgat atcgcctgta tcggcggacc ggttctcaag tggtacgaga
    15601 atctcggacg ttgagtggtg ttcttcgtcc aggtatcgat ggcgtaaaat cgaagcttgc
    15661 ctgttcccca ggtcaccgtc gtgattccga cgctcgtcgc ggactccgcg ctcgatgatt
    15721 gtctgcgtgc cttgggagag caaacgtttc gcaatttcga ggtgattgtc gtcgacaaca
    15781 gtggaaacgg gctagcccgc cgcagcgcgt ccataatcgc cggcacggtg gtcatcgaca
    15841 accccgccaa cgtgggtttc ggcgccgcga tcaaccaggg ctgcatcgcc tcacgcgcta
    15901 ggtacctcgc cactctcaat gacgacgcgg tgccgcatcc gggctggctt gctgctctga
    15961 cgtcggccct ggaggcccgc ccggacgcag gcatggctgc ctcgcggatc ctcctttcgg
    16021 gcacgggacg tctggattcc gccggtatgc tggtttgcgc ggacggctcg agcaaacagc
    16081 gcggacacgg cgagctgccc accagatttc ccgatccgga ggaggccctc ttccccagcg
    16141 cctgcgctgc cctctatcgt cgctccatgc tcgaggacac ggaaggcttc gacgaggatt
    16201 tctttctcta ctcggaagac acggatctcg gccttcgtgc gcgttgggcg ggttggaagt
    16261 gcctctacgt tcccggcgcc gtcgtggaac accgttattc gcactcagcc ggccgagcct
    16321 ctccgctcaa ggcgtactac gtcgagcgta accgcctctt cgtgatcgcc aagagtttcc
    16381 cacttccggc gctgtgcaga gcaccgttcg tcgcgttcgc ccgctacttt tggcacgcat
    16441 ggttcctgct tcgcgggcgt ggaaccgccg ctcgcttccg cgaggaaggc cacggcgctc
    16501 tccggctcgc atggtacatc ctctcggcac acgtggcgct ggttgtacgc gcacgttccc
    16561 tttggaagaa acggcgggaa atccgcgatg gtgcgcgcat cacaaccgcc gagttcttga
    16621 ggctcctgcg ggtgcattcg atctccgccc gggaggtcgc atccctgtga ctcccgccct
    16681 tcacgaccgc gacctgctga tcatcgtccc cgcctacaac gaagcggcgt cggtcggcca
    16741 ggtcgtgcac gaagtccgcg aggcggttcc tggagtgccg gtggtggtca tcgacgattg
    16801 ctcgacggac gggacctcta cggtcgcgcg cgaggccggc gcctcggtgt tgtctctccc
    16861 gcatcacctc gggctgggcg gcggcgtgca ggcgggctac aaactggcct acgagctcgg
    16921 ctacgagtac gtcatccgtg tcgacggcga cggtcagcac gacccgcgcg acatcccgcg
    16981 catctacgag gctctccgca ccaacgccta tcagatggtt atcggctcgc gcttcctcga
    17041 gaacgatgcg gaccagacca gtttcggccg ctctgtcggc gtgcgtttct tccggatcgt
    17101 tctgcggccc atcctcggca agccggtccg cgacccgacg tccggtttcg tgggagtgaa
    17161 ccgtcaggcg ctgctggtct tcagccggag ctttccgctc gagtaccccg aaatcgaagc
    17221 gcttgtggtg ctccagcgtc gcgcctttca gttcgcggag attccggtcc gcatgcgccc
    17281 gcgcctgggc ggccgcagtt cgatcacagc tgtgaaatcc atctactata ttcttcatgt
    17341 actgctcggc gtcttcgtga acgtcctcaa attcgatcgc cggcggtgga agagatagcc
    17401 gtggaccgcc tcctcaattt catgaccctg ctcagcgtgg cgctgatcgt gctcgtgctg
    17461 cgctcggtgc ggcgcgctca cattcgcgtc gaatattcgg tgagctggct tggcgccgcc
    17521 gtcacgctgc tggtgctatc gcgctcgaac tgggcgctcg atagtatcgc ccggctgctc
    17581 gggattgcag acccgccgct cgctctgctg ttggtcgtgg gctgcatctt tttgatcgtc
    17641 ctgtaccgct tttcgaccgt catctcagat ctgaaggacg cgaacatctc gctcgcccag
    17701 cgcgtcggga tcctcgaata ccatctccgg aacttgcatg aaaaccagga aaacccaaag
    17761 ccccgctaaa gcgtcgccgg gcttctcttt gaagccctgg cacgttgcgg tgggggtgct
    17821 ggcagcactg ttcgctctct tcgaatcgta cgggcctgca ctcaacggcg tgtttgtctt
    17881 tgacgacgag agccttccct tttactcccg tgacttcggc gcccggccgc tcatcccggc
    17941 catcaccggt gttcgcccat tcctgatgtt caccttctgg ctgaatcacc aaatctcggg
    18001 acttgagccg tatggctacc atctcctcaa cgtcctgttc cacttcgcct caggagtcct
    18061 ggtgtttttc atttgccgaa aagttctcga gtgggcggcc gtggcaggtg ggcgccgcga
    18121 actgttggcc gccttcgccg gctgtctgtt cctgttccat ccgatccaga ccgagtccgt
    18181 tgcctacatc gccagccgtt cggagaatct aagcatcctg ttctgttatg cggctttcgc
    18241 gctgttcctc tatcgccggt cgcgggcggt gtcctggcct gtcgtcgcag gcgttctggt
    18301 gttgtttgca gccgccgcat ccacaaaaga gcacaccatc gtcctcccgg cggtgctttt
    18361 gctaacggac tactactgga atccgggctt ctccttcgag ggcgcttgca agaactggcg
    18421 gctctatgga acgatggctg tcgtcggcgc tgccggcgtc tactccgtgt ggaacctgtt
    18481 gcgcacggcg cccaccgctg gttttggcgt gaaagaccta ccctggaacc attacctcta
    18541 cacccagtgg agagcgatgt gggtctattt tcggctactt gtgttcccgg ccggccagaa
    18601 tgccgactat cgatacccag tttcgcgcac tccgctggaa cccggcacgc tcattggtct
    18661 ggctgttctg ctcgtagcgg tcggccttgc aatccggttt cgacagcgtt acccgctcgc
    18721 ttcgtacgga tttctcgcgg cgctgttgct gtttgccccg acctcgtccg tcataccgat
    18781 tcgtgacgtc gttgcggagc gccgcgtcta ccttcccatg atcggcttgc tcttcgtgat
    18841 gcttgaattc ttgcgtcgct ggaagctgga cctgcgtcga atggcggcgg tgctctcctc
    18901 catcctgctg gtgtgtgccg tcctgacgta tcagcgcaac aaagtgtggg cggggccggt
    18961 acccctttgg gaggacgtgc tggcaaagtc gccgcaaaac tcgcgcgccc actttcagct
    19021 tgcgctcggc tactacgaag agggacgctg cgaggaatcg gtgcgacact acgagaccgc
    19081 cgagcgcctg ggagaacggg agcatcgtct ctacgtggat tgggccctgg ccgacgactg
    19141 ccttggccgc ttcgatggcg ctctcgcaaa aatcaaacga gccatcgaga tcgagaagac
    19201 ggcgcacgcg tatgcgctta tgggcatgat ctacgccaaa cagaacaggc gggatgaggc
    19261 gatgaccgca ctcgacgagg ccgggcgcat cgacgcgggc tacgacatga cgtacgccta
    19321 ccgaggcaac ctcctcgttc tggccaatga cttcgccggg gccgccgctc agtaccggcg
    19381 tgcgctcgag ttggacccca acaaccagtt ggccaggaac ggtcttgccc gcgttcaggc
    19441 ggctcttggc gccgcgcgat aggaggcttt tctgcgcatc ggagtcaatg ccctgtacct
    19501 ggtccctgga ggggtcggcg gcacggaaat ctacctccgt catcttctcg ccgcgctcgc
    19561 gggaattgac tccgtcaacg agtacgtcat cttcaccaac cgcgagaccg gtgcggatct
    19621 cgttccagtc cggccgaact tcgttcatga gccacagtcg gtccgcgcgg cgagtcgccc
    19681 cgcgcgcttg ctttgggagc agaccgtgtt gccattagcc gtccgtcgcg cccgcctcga
    19741 cgtcctgttc aatccgggct tcacctgtcc tgccctttgc tcatgcccgg ctgtgacggt
    19801 gttccatgac cttcagcaca agcgtcaccc ggagcatttc cgctggttcg acctgccgtt
    19861 ctggcgcatc ttcctgtacc tcgcagcgcg ccgctccaag ttcctcattg ccgtctccga
    19921 cgccacacgc aaggatctgg agcgtttcta tcaccttgct acggagagga tccgcgtcgt
    19981 cccggaaggc gtggacgagc gtctcttcga aatcggccgg gaccgcgctg cgttcgaagc
    20041 agaatcattc ctcctggcgg tttcgacgct acatccgcac aagaacctgg accgcctcat
    20101 ccgcgtcttc tcccgcttcc acacggacag gcccgatttc cggctggtaa tcgtagggct
    20161 ccgcggtttc catgcggagg agatcgaaaa ggaagtccaa gaactgggac tgggcggcgc
    20221 gatcacacta acgggttgga tccctcgcga ccaactgtat agcctgttcc ggcgtgcgca
    20281 ctcgttcgtc tacccgagca ccttcgaagg attcggtctg cccgtgctgg aggctctcgc
    20341 agcgggcatc ccctccgcct gttcggccat cgagccgctg atgtcgctcg ccggagacgc
    20401 ggcgctgcag ttcgatccgc aagacgagga tgcgatgctc gaggcaatcc ggcgccttac
    20461 gtgcgatctg gcgcttcgag gccaactcgc gcaacagggc ccccgccgtg ccgctgcttt
    20521 ctcgtggaag aagaccgccg aagccacgct cgctgccctg cgcgacgcgg tatttaaccc
    20581 gcgcgcgtga tcgggacggt ttcccgccct accgaagcag atcctcgagc tggatcttcg
    20641 cgtcggtctt tgcgtcgcga tactttacaa tcaccggtgt gtacaacgag atgccccaat
    20701 ccttgcctgg accgtgagga atggctcgtg caccggcgac aaccactgcg ttctccggaa
    20761 cgataagcgg ctgatcgtcg gtcgctgtgt agacctcgcc tttcacgatg tcgtacaccg
    20821 gcgtggagcg gttgagaatc gtgccagtcc ccaagacgac gcggcgcttc acaaccgtcc
    20881 cttcatacac tccacaattg ccgcccacca gcacctcgtc ctcgatgatc accggcaacg
    20941 caccaacggg ttccagcacc ccgccgatct gggccgccgc cgaaatgtgg cagttcctgc
    21001 cgacctgcgc acagcttccc accaaagcgt gcgagtcgat catcgtccca tcgccgacgt
    21061 aggacccgac gttgatgtac atcggcggca tgcaggtgac gccgcgtccg acgtagcagc
    21121 cgtcgcgaat actggatcca cccggcacaa tgcgcacgcc gctctccacc gtgagccgct
    21181 tcaccgggta cgtcgccttg tcgaagtatg gctgccgagc ggtgttcacc gacatgtcca
    21241 ccagcgcgcc catgcggaac ccgagcagga tgcccttctt cacccagccg ttcacccgcc
    21301 agccgctctt cgcggatgca tccggttcgg cggcgcgcgc ttctccggcg ttcagcgcgt
    21361 acttgaaccg ctgaaacagg cggaagtgct cctcagtgta ttgctcgggt ttggcgtcga
    21421 acagagcctc gatttcagtt gccggcatgg atggctttgg gttcgagcag agcgaggtcc
    21481 tgaagcacct tgtcgatctt cgcgaggtta accaccgacg gcggcacgag cggcaggcgc
    21541 cagactggtt cgaggagccc catcagcccc atagccgcct tcactggaat ggggtttgac
    21601 tctacgaagt tcacgtccat cagcggcagg tacttgcggt gaaccgcacg tgccgccgca
    21661 aagtcgcccg acaggcataa ctgcgccaac cgtgtcattt ccgctggaat ttcgttcgat
    21721 gcgaccgaga ttaatccccg cccgcctaac gccatgagtg gcagcgtaat cgcgtcgtca
    21781 cctgacagca ccgtgaatcg ctcgggaacg agattgagga ttgccgacat ctggctgatg
    21841 ttgcccgacg cctctttgac cccaaagata ttgtcgatct ccgccagccg tttcaacgtc
    21901 gccggttcaa cgttgacgcc cgtccgcccc tggacgctgt acacgatgat cggcagcctg
    21961 atcgccgcgg cgatcgcctt gtaatgctga tacagcccct cctgcgtcgg cttgttgtag
    22021 tagggtgtta cggagaggat gccgtcggcg cccatcgact cgagttgctt cgcgagtgag
    22081 atcacctcgc tggtgttgta accgcccgcg cccgccagca ccgggacttt gcccttggcc
    22141 tcgtcgagcg tgatctcgac cactcgaagg tgttcggagt gctcgagagt cgggctctcg
    22201 ccggtcgtgc cgcagggaac gaggaagttg attcccgccg cgatctggcg ccgcaccagt
    22261 cttcgcaaag tctcttcatc cagcgacagg tccttccgga acggtgtcac cattgcggtc
    22321 ccacaacctt tgaacattct tcacctcctc tgcgtgtccg tttcctcttg gctcaacgac
    22381 ccgccattat ctcactgaac tcgaagaaac ccttcttgcc aaccacccac tgcgccgcct
    22441 tcaatgcccc ccgcgcgaaa ccctcgcggt ttcgtgccga gtgccgcaga gtgatcgtgt
    22501 cggcggctga gtcgaagccg atctcgtgcg tccccggatg ggtccccgcg cggtttgacg
    22561 atgcatcgat cggccgcccg tagccggctt tcttcatctc gccgaacagt ttcagcagcg
    22621 tgcccgaggg tgcgtccttc ttcgtcgagt gatgaatctc ccaaccccac gcaccgtaac
    22681 tcggctcgtc cttaagcagg cgtgccgcct cggccaccag ccggagaaac acgttgacgc
    22741 cgatcgagaa gttcgggctc cacaccattc cggtgcccgc cttttccacc accgatttga
    22801 catggtctaa ctgcccgagc cagccggttg tgccttcgac gacgttgacg cccagcgcag
    22861 aaatccgctc tacattgctc actaccaccg gcggaatcga gaagtcgatg gccacatcga
    22921 cacctcgaaa attgtccgcc gtgatgccct cgtagttcgc gttgttgaac tcgtccaact
    22981 tcagcgtaac ggtgaagcca tactccggag cgagctgttc aatcagccgc cccatcttgc
    23041 cgtaacccac gatggcgagg ttcatgtcag tcgaagaccg ccggatccac ctcggcaaag
    23101 aactcacggt gcagcgcgtc gaccgtcttc gccaagtcct gctccgccac cacgaaactg
    23161 aggttcagca acgaagcacc ctgcgagatc atgcgcacgt taacgtcctc gagcgcatgg
    23221 aagacccgcc cggacactcc cggagtgtat cgtatgttct cacccaccag gcacacgatc
    23281 gcctggttgt tctcggtgtc cacctcggcg aactgccgca actcttccag gatcgccggc
    23341 agccgaccgg gattgtcgat cgtcagcgag acactgactt cggatgtcga gaccatgtcc
    23401 acaggcgtct cgtagcggtc gaatgactcg aaaatccgcc gcaggaaacc gtgcgccatc
    23461 agcatgcgag ttgagcggat gttcacgaga gtgatcttcc gcttgcacgc aatcgacttt
    23521 accgcgttac ggcagggaac ggcctcggcc acgattctcg tgccaggcac atccggccgg
    23581 cgcgagttga gaatcagcac cggaatattc ttctcgatcg ccggcgtcac cgtcgccggg
    23641 tggagcactt tggcgccgaa gtacgcaagt tcagccgctt cggcaaatga gatcgtcttt
    23701 acgcggtgtc cgcagggcag gatcgtagga tcggcggtga gcatgccgtc cacgtcagtc
    23761 cagatctgaa tctcctccgc gctgattccg gcgcccacga tcgaggccgt gaagtccgag
    23821 ccgccacgcc cgagcgtgct cgtcacgccg gcttcggtgg agccgatgaa accacccatc
    23881 acgacgatct tccgctgggc cagcggcgcc accttcccgg ccagccgctc atacgactgc
    23941 gggtagagcg gcgctgcctg cgtgtggcgg gcgtcggtta cgatcaccgc ccgcgaatcg
    24001 atgtggactg catccattcc gaaataccgg aacgccagcg tcacgagtgc gctcgacaag
    24061 cgttcgccaa atgctgagat tgcgtcaatc gaccgcggcg tcagttcccc caacaccggg
    24121 aatccgcctg acagttcggc gagctctcca aagtgctcgt cgaggatgcg ccgcgcctcc
    24181 ggccggtccc cctcgcgaag gatcggatcg atctcccgct gatggaaggc ccgcagctcg
    24241 gccagttggt cgagcgcctg cgcccgccgg ccgttcaccg catcgctggc gatggcgagt
    24301 aacttgttgg tcgtcttgcc catcgcggaa acgaccacca ccggccgtcg ctcagcccgc
    24361 gcctttacga tcgatgcaac ccgttcgatg gcggccgccg actccaccga cgtgccgccg
    24421 aatttcatca cgatcatcag tcgagcaatc cttccgaaac cataagctcc gcattcagta
    24481 cggccgcgcc cgccgcgccg cgaaccgtgt tgtgtccgag ggcgacgaac ttccagtcga
    24541 gcaccggaca ctccctcagc ctgcccacaa agaccgccat accgcgctcg cgctccacgt
    24601 cttttcgcgg ctgcgggcgg ttcgcttccg gcatgtaaat cactggtctc gccggagcgc
    24661 tcggaagccc ccgcttctgt ggctcactag tgaacgtgtc gaatgcctgc cgtaactctg
    24721 cctccgtcgg cttcgccgac aactcaaccg agacggtaac agtgtgtccg tctaccaccg
    24781 gcacacggtt gcagtgcgcg ctcactttgg cttcaagaga atggatggca tcgccgttaa
    24841 cgtagccgag gatcttctgc gtttcgacct gcatcttctc ttcttcgcca ccgatgaacg
    24901 gaatcacgtt ggcgttgata tccatcgagg gcacgcccgg gtagccggcg ccggagattg
    24961 cttggagcgt cgtggcaatc acgcgagtga tgccgaacgg tttgagagcc gccaacccca
    25021 tcgtcaacac gaccgtcgag caattgggat tcgtgacgat ctgtccggtc caaccccagc
    25081 gatgctgctg cgcggggatc accttcagat ggtcggggtt gatctcgggc accagcagcg
    25141 gcacatccgg ctccatgcgg tggttccgcg agttcgagac gacgacgtgc cccgactggg
    25201 cgaatgctcg ctcgatctcg gtcgcgacgc tggcatccgt cgccgagaac agcagtttcg
    25261 gcgcattacc cggcttgcac tcatcaacga taatgtccgc caccgtctcc ggcgttacac
    25321 cgccgaggtg ccactggcag gcttccttgt acttcttgcc cgccgagcgg tcgctcgcgc
    25381 cgagccacgt cagctcgaac cacggatgat tctggagaaa cttgacgaaa tgctgtccca
    25441 ccatcccggt ggcgcccaaa attccgactt ggatctttcg ttccattacg cttccttctc
    25501 tttggcgagg agctgcctca ccatctgttg atagatctca accgcttcca cgagttccgt
    25561 cttcggcacg cgctcgtcga gcgtgtgtgc gacgtggatc gtacccggtc cgatcaggaa
    25621 cggggtaccc cacgcaccct caaacgccgg gatgtcggtc gtgtacgaga cgacagtagt
    25681 gtcgtatccg tcaagcgagc cgagttgcag cgcgggaatg cagagcactt cgcgcgcatc
    25741 ggcgcgacca tccgcggcct tcttcacacc ctcccgcgtg ggacccgggt cgctcaccag
    25801 ccggatcatc acctcggcct tggcggaatc cgggatcacg tttggcgcgc gccctcccgt
    25861 aattgttccg atgttcatcg tgctccgccc gagcaccggg tcaacaggca ggggcatctc
    25921 gcgaagctct tggagtacgt cgagcagctt ttcgatcgcc gattcgccaa gctcggggta
    25981 cgccgagtga gccatccggc cctgcgccga gatctcgtag cgcaacgcgc cctttgatcc
    26041 caacgcgagt ttgttcccgg tcggttcgcc gttgatcagg taccggctgc cccgcggcat
    26101 ccgggcggcg gccatcgcgc ccgcactgtt gcgttcctcg ccgacgacga acagcagtcc
    26161 gaagttgcgg accccgtccg cgagcagccg ttccgcggcg gtgatcatcg aggcgatgat
    26221 acccttcgtg tcgcacgcgc cccgccccca gatcgattcg ccgtcttcac ggctcggcac
    26281 gtgcggcggc accgtgtcca tgtgagttga cagcgtcacc accggctcgc cccagcacgc
    26341 gaaaacgttg aaacggtccg gttcgatttc cacgctttcg gcccgcccgc cgtgtttggc
    26401 agccagggtc gcgaggagat cgttgaggaa aatcccgacc tgcctttcgt tcccggtgat
    26461 tgattcgagg tcaatcaggg tgcgcgtcag ctcaaacaag ttcataagtg tcgttctatt
    26521 ggatggagcg ggggagagag aagccgcaaa ggaaggaaac ggcgtttcta cacttcccga
    26581 tagcgctcca tccgctttcg acggatgaca gtgggcaggt cttaacctta ccccccagtc
    26641 gccccggagt gtgcttcccg atgcgacttc ggcggattca ac
//
LOCUS       DATIFQ010000209         5980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2560744, whole genome shotgun sequence.
ACCESSION   DATIFQ010000209 DATIFQ010000000
VERSION     DATIFQ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2560744"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..874
                     /locus_tag="VLE22_09880"
     CDS             473..874
                     /locus_tag="VLE22_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSB14759.1"
                     /translation="MNRLLWVVQALLALLFLFAGGTKLFMSAAELTAQTPIPAGFLRF
                     IGVMEILGGLGLVLPGMLRIRTALTPLAAVGLSIIMIGAVVVTIQTMGSALATLPFVT
                     GALAVFVAYGRWRLAPLSAPHATARRQSMHS"
     gene            1226..1855
                     /locus_tag="VLE22_09885"
     CDS             1226..1855
                     /locus_tag="VLE22_09885"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HSB14760.1"
                     /translation="MGKLAPLQKKYNGGKMRTGRLQAFSDGVIAIIITIMVLEMKVPH
                     GDSLDDLLPVLPVFLSYVLSFVYVGIYWNNHHHMLHATTTVTGAMLWANLHLLFWLSL
                     FPFATGWMGENHFSAVPTALYGVVLLMAAIAYYLLEQAIIRSQGPDSILKKAVGRDWK
                     GKLSPVLYIAAIVATLLSPWIAEAIFVAAALIWLIPDRRIEKVLPRKEV"
     gene            2009..2347
                     /locus_tag="VLE22_09890"
     CDS             2009..2347
                     /locus_tag="VLE22_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSB14761.1"
                     /translation="MSKQRTDVPYGTLDLLVLKTLETMGEMNGYRIARRIEQVSGDAL
                     KLSQGAIYPALIRLEQEGWIRAEWGISETNRKVKFYSLTKAGTKQLEAEVQKWEQANA
                     LLARFLEAAP"
     gene            2344..5010
                     /locus_tag="VLE22_09895"
     CDS             2344..5010
                     /locus_tag="VLE22_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14762.1"
                     /translation="MRWRGRIERVLALVRGRRLDRELEAEVEAHLEMAETDARKAGLS
                     AEEAWRAARRRFGGVEQMKEEHRDRRSVRWIETGVRDFRIAVRTLGRAPGFVVVAVLT
                     LAVGIGATAAVFSLIQGVLLTPPPYKDPEQLVLIPSARTDGQQQESPRPWPAAQWLEW
                     QREAKSVQSIAAYRWMFNFVILPDGVESFEAMAVTPEYFSVVGLEPVLGRKFVASDTA
                     PSAAPVLMIGYELWQRSFGGNPDILGQTVRVGRQDAPFEIVGVMPPGVRFLPAPNAAQ
                     EPNYDVNALVDIWAPFAPGSDPKDLKRPRWNVIGRLAPGATPERAQAELALITARQAH
                     ADRDFAGFTPDVQLLTSEFNRDGERILLPLFGAAALVLVITCGNVAALLLLRGLGRQR
                     EYAVRAALGAGRAGLFRQASTESLLLALAGSAFGIGLALGAVRAFKLIGGQAIPRLDA
                     VTTGWPILLFGLALALIAATLAGLIPAWRASRLDPNEVLKGAGPKSTAGRGERHLLRT
                     VTMAQTALTLVLLVGAGLLIRTMINISKAPSGYKTSHILAMTVTSVEGDWSDFHHRAL
                     DRVSTLPGVRHAVYAWGVPLTGNNWPARVIIEGQPVPVRESEATEVPLRSITPGYFEL
                     LGMAVTEGRDFRSNDDDDAPQVAIVNKAFADRYFPDTTTLGKKVWLFGPDRAPSKIIG
                     VVANSRTDDLTRQASPEIYMSFWQTRAYSKHLLVRTEAEPLAMASAVHAALREVNPYA
                     AVEHIKTFDQIRNDSLAGRSFAMQLLVGFAAVGCLLTLVGIYGVLSLSVASRRHEIAI
                     RTAVGAKRRDIRNLILFDGFRLIAGGVAAGLAGALVLSRVLRSFLYEVEPNDPATLFA
                     VAMLFIGVALLACWPPTRRATRVDPMKTLRCE"
     gene            complement(5490..>5980)
                     /locus_tag="VLE22_09900"
     CDS             complement(5490..>5980)
                     /locus_tag="VLE22_09900"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB14763.1"
                     /translation="EETNMQPKNTICLWFDKDAQEAARFYAATFPDSKVTAVHEAPGD
                     YPGGKKGDVLTVEFTVLGVPCLGINGGPVFRHTEAFSFQIATENQEETDRYWNAIVGN
                     GGAESECGWCTDRWGLSWQITPRALTDALAAGGGEAKRAFEAMMTMKKIDVAAIEAAR
                     RG"
BASE COUNT         1077 a         1907 c         1851 g         1145 t
ORIGIN      
        1 cctgtgccac aaggatattg acgccctacg taacttccgg cgccacggtt cggtgggctg
       61 ccgcaccctg acggtcgcgg ttcggaaact gcatcgaaca tgggggcagg tttctaaccg
      121 cgtacgcgag gcgcaggcgc aacgacaggc ggaaaagcgg ggacaggcac caatttcgca
      181 aacaccgcga aattggagcc agtccccgtt tttcgtgcaa acaccgcgaa atttggagtc
      241 aatcccgggc tttgctacgc acaggctgaa gcctgtgcca cagggatatt gccggcgcga
      301 aggacgcaac ggaggtgtga gggagaggac aagatgcccg gcaaggtcat tgcagccaac
      361 cattcccgat tacggattga tatcatgtac ccgatgctgc cttgcctggt tgaactacgg
      421 attttgcggc tgggactggc ttcgacgtga atcgcattcg aggcgataag acatgaatcg
      481 gctgctttgg gtagtccagg cactgctcgc gctgctcttt ctttttgccg gcggaacgaa
      541 gcttttcatg tcagccgcag aactgacggc ccaaacaccg atacccgcag ggttccttcg
      601 attcatcgga gttatggaga ttctgggcgg cctcggcctg gtactgccag ggatgctgcg
      661 cattcgcacg gcgcttactc ccctggccgc cgtaggtctt tccataatca tgattggcgc
      721 cgtagttgtc accatccaga cgatgggctc agcactggct acactgccgt ttgtaaccgg
      781 cgcgcttgca gtgtttgtgg cttacgggcg ttggcgactg gcgccgctca gtgcgccgca
      841 cgcaaccgct cgccggcagt ccatgcattc gtgatcggaa ccgctcaacg tcctgactga
      901 acggggcctc ctggagtatg cggtacaccg aatggagcgg acaggcggcc tacctgggga
      961 gatgggacag gcagctctgt ccgcttcgca aactgcgctc aggacagagt aggcctgtcc
     1021 cgctcggcaa aacccggctc tggggagcgg ccgtaacgca actgccggca ggactgccgg
     1081 cacggcaagt ctggcgactt gcgccacggg ttcgctggag cttccgctgc ctgacagtcg
     1141 cggttccgta acggcatgcg aactcagtgg ctaggggaga tgggacaggc agctctgtcc
     1201 gctcgcaaac tgcgctgtcc gggacatggg taaactcgcg ccgctgcaga agaagtataa
     1261 cggagggaag atgcgcacgg gacggttgca ggcattcagc gacggcgtga tcgcgatcat
     1321 catcacgatc atggtgctgg agatgaaggt gcctcacggc gacagcctgg acgacctgtt
     1381 gcccgtgctg ccggtgttcc tcagctacgt cctaagcttc gtttatgtcg gcatctactg
     1441 gaacaaccac catcacatgc tgcatgccac caccacggta acgggcgcga tgctctgggc
     1501 gaacctgcac ctgttgttct ggctgtcgtt gtttccgttt gccactggct ggatggggga
     1561 gaaccatttc agcgcagtac cgaccgcgct ctacggcgtg gtgctgctga tggccgcaat
     1621 cgcgtactac ctgctcgagc aggcgatcat ccgctcgcaa gggccggact cgatcctgaa
     1681 aaaagcggtc gggcgcgact ggaaggggaa gctgtcgccg gtgctttata tcgcggcgat
     1741 tgtggcgacc ctgctttcac cgtggatcgc cgaggcgatc ttcgtggccg ctgcgctgat
     1801 ctggctgatt ccggaccggc gtatcgagaa ggttctgcca cgcaaggagg tctagcgatc
     1861 tccgggccgg cacatttgtc atttaaatgg taaagtgacc ccgattggcg gcatataggg
     1921 cagcaacaac gtaccttcgg taggctaccc aacccattga cagacagcca tctgttggtt
     1981 gatgattgta gacatgtcta atgaagacat gtcgaaacaa cgaactgatg ttccgtatgg
     2041 caccttggat ctcctcgtcc tgaaaacact ggagacgatg ggagagatga atggctatcg
     2101 catcgcccgg cggattgagc aggtgtcggg cgacgccctg aagctgagcc agggcgccat
     2161 ctatccggcg ttgatccgct tggaacagga aggctggatt cgggccgagt ggggcatttc
     2221 cgagacgaac cggaaggtga agttttactc gttgaccaag gccggcacta agcagcttga
     2281 ggcggaggtc cagaaatggg agcaggccaa cgcgctgctg gcgcggttcc tagaggcggc
     2341 tccatgaggt ggcgtgggcg aatcgaacgt gtcctggcgc tggtgcgggg ccggcggctg
     2401 gatcgggagc tggaagctga ggtcgaggcg cacctggaga tggctgaaac agatgcgcgg
     2461 aaggcaggat tgtcggccga agaggcctgg cgggcggcgc ggcggagatt cgggggagtc
     2521 gagcagatga aggaagagca tcgggaccgg aggagcgtgc gttggatcga gacgggggtg
     2581 cgcgacttcc gcattgccgt ccgcacgctc ggccgcgcgc cgggtttcgt tgtagtggcc
     2641 gtgctgacgc tcgcggtcgg catcggcgcg accgcggctg tcttcagcct gatccaggga
     2701 gtcctgctca cgccgccgcc ctacaaggac ccggagcagc ttgtcctcat cccctccgct
     2761 cgcacagacg gccagcagca ggagagcccg cggccttggc ccgcggcgca gtggctcgag
     2821 tggcagcgcg aggcgaagtc cgtccagtcg atcgctgcct accgctggat gttcaacttc
     2881 gtgatcttac ccgatggcgt ggaatccttc gaggccatgg cggtcacgcc ggaatacttc
     2941 tctgtcgttg gactcgaacc ggtgctcggc cgcaagttcg ttgcttcgga caccgctccc
     3001 tccgccgccc cggttctcat gatcggctat gagctgtggc agcgcagctt cggcggcaac
     3061 cccgacatcc tcggccaaac cgtccgcgtc ggccgccagg acgctccgtt cgagatcgtt
     3121 ggtgtaatgc cgcccggcgt tcgtttcctc ccggctccca atgctgcgca ggagccaaac
     3181 tatgacgtga acgcgctcgt ggacatctgg gcgcccttcg cgcctggctc cgatcccaag
     3241 gacctcaagc gcccgcgatg gaacgtcatc gggcgcctcg cccccggcgc cacgcccgag
     3301 cgggcgcagg ccgagctcgc tctaatcact gcgaggcaag cccatgccga tcgcgacttc
     3361 gccggcttca cgcccgacgt ccagttgctc acctctgagt tcaaccgcga cggcgagcgc
     3421 atcctgctgc cgctgttcgg cgccgccgcg ctcgtactcg tgatcacctg cggcaacgtc
     3481 gccgcgctgc ttcttctgcg cggactcggc cgccagcggg agtacgccgt ccgggccgca
     3541 cttggcgccg ggcgcgctgg gctcttccgg caggcctcca ccgaaagcct gctgctcgct
     3601 ctcgccggca gcgcgttcgg tatcggcctc gcgttgggtg cagtgcgagc attcaagctg
     3661 atcggcggac aagccattcc acggctcgac gctgtcacca ccggttggcc gattctgctc
     3721 ttcggactcg ctctcgccct tatcgccgcc accctcgccg gactcatccc cgcctggcgc
     3781 gcctcgcgtc tcgacccgaa cgaagtgctg aaaggcgccg gccccaagtc caccgcagga
     3841 cgcggggagc gccacctctt gcgaaccgtc acgatggcgc agaccgcgct cacgcttgtg
     3901 ctgctggttg gcgccggcct gctgatccgc accatgatca acatctcgaa ggctccgtcc
     3961 ggctacaaga ccagtcacat cctcgcgatg accgtgacct cggtggaagg cgactggtcc
     4021 gattttcatc accgcgcact cgatcgcgtc tccactctcc cgggcgtccg gcacgccgtt
     4081 tacgcctggg gcgtcccgct caccggaaac aactggcccg cgcgtgtcat cattgaaggc
     4141 caaccggtcc ccgtgcgcga gagtgaggcg accgaggttc cgctgcggtc gatcactccg
     4201 ggctatttcg agctgctggg gatggcggtg accgaaggcc gcgactttcg ctccaacgat
     4261 gacgacgacg caccgcaggt cgccatcgtt aacaaggcct tcgccgaccg ctactttcct
     4321 gacacgacca cgctcggcaa gaaggtctgg ctgttcggac ccgacagggc gcccagcaag
     4381 atcatcggtg ttgtcgccaa cagccgcacc gatgacctca cgcggcaggc ttcgcccgag
     4441 atctacatgt cgttttggca aacgcgcgct tactccaagc acctgctggt ccgcaccgag
     4501 gccgaaccgc tcgcgatggc ttccgccgtt catgccgcgc tccgcgaggt caacccctac
     4561 gccgccgtcg agcacatcaa gacattcgac cagatccgca acgactctct tgccggccgc
     4621 agcttcgcca tgcagcttct ggtcggcttc gcggcagtgg gctgcctgct aacgctcgtc
     4681 ggcatctacg gcgtgctctc gctttcggtg gcctcacgcc ggcacgagat cgccatccgc
     4741 accgcggtcg gcgccaagcg ccgcgacatt cgcaacctga tccttttcga cggattccgg
     4801 ctcatcgccg gtggcgttgc cgcgggactc gccggcgcac tcgtgctttc gcgcgtactg
     4861 cgctcgttcc tctatgaggt ggagccgaac gatcccgcaa cgctcttcgc cgtcgccatg
     4921 ctattcatcg gtgtggctct cctggcctgt tggcctccga cccggcgcgc cacccgcgtg
     4981 gacccgatga agacgctcag atgcgagtga cacggcacgc agttgttgga agcgccacat
     5041 ggatgattcc atccacatgg acgtataacg ccccatactg agatgtgaca gtgatctgaa
     5101 caaatcctgc ctgacgccga gagcatttgt cccagctcca gccgtatggt cggcaaaccg
     5161 gaagtaatcc actctgtccg ttaggcggac tacgcaactg ccggcaggac tgccggcacg
     5221 gcaagtctgg ggacttgtgc cacgggttcg gtgggctgcc gctccctgac ggtcgcggtt
     5281 ccgtaactgc atgcgaacac agtggctgga agaaatgggg acagacagcc atttcgcaac
     5341 aacggcgaaa tggtggtcag ccccccattt cttcagaaac ttcgagtcaa tccggggttt
     5401 tgctacgcaa ccacaccgaa aatgatcagt taaggaccgc aacggaggta tgagggagag
     5461 gacaagatgc ccggcaaggt catttcacgt cagcctcgcc gcgctgcctc gatggcggcg
     5521 acgtcgatct tcttcatcgt catcatcgcc tcgaaggcac gctttgcctc accgccacca
     5581 gccgcaagcg catcggttag cgcgcgcggg gtgatctgcc aggagaggcc ccagcggtcc
     5641 gtgcaccaac cgcactcgct ttccgcgccg ccattgccga cgatcgcatt ccagtaacgg
     5701 tccgtctctt cctgattctc cgtcgcgatc tggaaggaaa aggcctcggt gtgcctgaac
     5761 accgggccgc cgttgatacc gagacaggga acgcccagga cggtgaactc caccgtaagc
     5821 acatcgccct tcttgccgcc cggatagtca ccgggagcct catgaactgc ggttactttg
     5881 ctatccggaa aggtggcggc gtagaagcgc gccgcctcct gcgcatcctt gtcgaaccag
     5941 agacaaatcg tgttctttgg ctgcatgttc gtctcctcct
//
LOCUS       DATIFQ010000210        15233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_645376, whole genome shotgun sequence.
ACCESSION   DATIFQ010000210 DATIFQ010000000
VERSION     DATIFQ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15233
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645376"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VLE22_09905"
     CDS             <1..823
                     /locus_tag="VLE22_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14764.1"
                     /translation="TCLVPFASTRLRAAAGFGGEDHTPGTFGFLTWGGDGGATAAENT
                     LLTVLGGCFCAGCEAAARKDGLDLKAVRRALRPLADMLDHAGPAETHRLALLRASNTT
                     PTALLLRHPELFEWMKFRCTSITRLFADAHAAVRAINPKIDLRLNAYISRNPELSGID
                     LASLKHHLGSVRSSDYSEQSGMASQLEHKRQFLLSIREAVGDEMHFLSAIGIRPKATP
                     ELIRQGVQISSECGADGLSLGHYDGAPLRNLDAIREGMAAADVVMRAGRIHKALL"
     gene            897..1448
                     /locus_tag="VLE22_09910"
     CDS             897..1448
                     /locus_tag="VLE22_09910"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB14765.1"
                     /translation="MRVVKIFAPLVCLFSLLALPLAAQWEESGDVPYVPTPPEVVEAM
                     LKLGSVKKTDVVYDLGCGDGRIVVMAAQKFGARAVGVDINPERIKEAQANAKEAGVTG
                     RVEFRQGSFFDADIKDATVVTLYLLSSINLKLRPKLLKELKPGTRIVSHSFDMGDWKP
                     DKEIEVSGRRIYLWHVPANAGSH"
     gene            1453..2184
                     /locus_tag="VLE22_09915"
     CDS             1453..2184
                     /locus_tag="VLE22_09915"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB14766.1"
                     /translation="MTADSGFCTLRIVIAGAAAIIVSACFGQKPDAQPREINPGGPGK
                     APSDAVVLFDGKDMSHWTTRDGKPARCQVQSGDMVCLTGVGDIYSREKFRSAQIHLEF
                     AVPFMPEQKDQMRGNSGVYLHGKYEIQILDSYHNPTYAVGACGALYGQSPPLVNASRP
                     PGQWQSYDIVFHAPRCDGAGKMMSPATVTVLQNGVLVQDHVKLENSTGCGEEGPLMLQ
                     DHSGFPGAPQTSMKFRNIWLRRLDE"
     gene            complement(2217..2768)
                     /locus_tag="VLE22_09920"
     CDS             complement(2217..2768)
                     /locus_tag="VLE22_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HSB14767.1"
                     /translation="MKQIILAVFCLALVLPATAATGTLKAHGKPGDAGLFVDGKYLGP
                     AVRYSFAVKYQVEEGTHEVVLQDPRYEDFKTTVTVKAGEVTDLKYKLTKLPEPKGPFG
                     TLKFGRGKPDTFMSMVVGDTSAVYLNGRFVGHLEEMNHVGTGLLLPPGKYELRCSSPM
                     YGEINETIEIVAGKTTKIKIPRE"
     gene            complement(2884..4395)
                     /gene="xylB"
                     /locus_tag="VLE22_09925"
     CDS             complement(2884..4395)
                     /gene="xylB"
                     /locus_tag="VLE22_09925"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HSB14768.1"
                     /translation="MRTSSMYWLGIDIGTGGSRALLVDEKGKVKAGFTAPHEDMRMEK
                     PLWAEQRPEDWWDAARKAIQGVMAEAKVSGKNIKGVGLSGQMHGLVLLDESNAVIRPA
                     LIWCDQRSQAQVDFINQRIGRSNVVAFTANPVLTGFTLPKLLWVRDNEPAAFARVRKM
                     LLPKDYVRFQLTGEYATEVSDASGTALFDVVMRRWSNQMADRLGVDRAILPKVYESSE
                     ITGQVTAEAAALTGLIEGTPVAGGGGDQASSAIGNGIVEPGIVSCTLGTSGVVFAHME
                     KATYDPQGRVHTFCHAVKGAWHVMGVTQGAGLSLQWFRNQFAPGAEYDALTAEAAGAG
                     AGSHGLFWLPYMMGERTPHLDSTARGGWIGITAKHTRADLIRSLLEGVSYSQKDCLDV
                     IEQCGVAVESVRASGGGARSPFWRQLLADTFGKRVVTLASQEGSAYGAAILAMTGTGA
                     YSSVPEACRAVIREVESVLPRPHESQLYRRGHEVYRTLYPTLRPIYGLIENLR"
     gene            complement(4392..5333)
                     /locus_tag="VLE22_09930"
     CDS             complement(4392..5333)
                     /locus_tag="VLE22_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSB14769.1"
                     /translation="MAEFSIGVDLGGTNLRAAAIDRDGNVLRKTSGGTKLSAGREAVI
                     DDIVASIVKLRDRLGADGLVGAGIGVPGFIVIEKGLVVGSPNLPGFEDFPVRDEIESR
                     LHAPVILENDANAAALGEKWMGAGRGVNDLVLLTLGTGIGGGVISGGKVLHGFVGMAG
                     EVGHITVNPAGNPCGCGNRGCLEKHASATAVVATARMLGLGDNITSEGVYRLAVEGNE
                     SAQRVFESMGSALGIALANLVNIFNFPLYLLSGGVLPAWDYFAPTMFRELETRSFTYK
                     KTRTRIEKAILGNEAGLFGAAYLPFQAKDQACKSKQS"
     gene            5394..6356
                     /locus_tag="VLE22_09935"
     CDS             5394..6356
                     /locus_tag="VLE22_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSB14770.1"
                     /translation="MGTVHVVFGAAGGLGAAIVRKLTAGGEPVRAVVRDAGKAKRLFA
                     EPVEVVAADARDALSTRLACRGASVVYHCVNVPYNLWREVLPAVTDNILEGARYEEAR
                     LVFSGNVYGYGYFDKLPVTENHPRAALTRKGSLRNELEDKLLEAHQAGDVQVVIPRMP
                     DFYGPNVTNKLYGGLFEAALAGKRATWLGNAELPHDLVFIDDAAAACVLLAATGSAYG
                     QAWHITSPETLTGREFLRLIYRTAGASLRVFVADRFLLRLGGLFDPVARELIEILYLF
                     EQPQVLDGTKFQQAFPEFRYTPHAEAVRRTLEWFRGRTTDRSGH"
     gene            complement(6379..7446)
                     /locus_tag="VLE22_09940"
     CDS             complement(6379..7446)
                     /locus_tag="VLE22_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14771.1"
                     /translation="MNAAKALLLVAATLGVWAQARLTVDQLISFVRSSVELKHKDKDV
                     AEYLRTVKLTQSLDEREVVELQAAGAGPKTIEALRELQRASKALPAPARKPPEPEKPS
                     IPPPSIEEQRQIMDKVREYALGYVKQLPDFICTQVTRRYIDPTGLEFWQRADVITARL
                     TYFEQKEDYKVILINGVTTDRPYESIGGATSTGEFGSMMREIFEPETQARFRWERWAT
                     LRGKRAHVFSYRVSKPKSKWRIDYERRLDVTPAYQGLIYVDRDTEMVMRITLEAEDIP
                     ADFPIQQASSVLDYDYTEIAGQQHMLPLKAVVRMRENKFLVKNEVEFRLYRKFAAEAT
                     VTFETPEPLPAEKTTEQPPKP"
     gene            complement(7451..8548)
                     /locus_tag="VLE22_09945"
     CDS             complement(7451..8548)
                     /locus_tag="VLE22_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HSB14772.1"
                     /translation="MRFRSFTIWIALLLSAPASWTQQNALLSNPDALKLFDRAVQLLD
                     STRVAVPELARAGAPVSENVRQAVVTLKATGAQNAGVTYTFLANLRAYLALSDSIPKP
                     FPFPDEARRQFAELRDTADRAESHFRALLELKESQLRNSDRDNLGRYAAANGKLPAPK
                     PDLPRVVFLGDSITDGWRLNEYFPDRDFVNRGISGQITGEMLGRMRADVIDLKPSYLL
                     VLAGTNDIARGVDVKTIQNNLTMIADLAEAAGIRPLFASLLPVSDYHKGDDPRYEQTK
                     RRPPATIRQLNAWIEGFCHQRGYTYVDYFSQLADTSGLLKEDSSDDGLHPNGKGYRLM
                     APVALAAIDKEIKAAAGKKPKRRRFPFFGGE"
     gene            complement(8627..9274)
                     /locus_tag="VLE22_09950"
     CDS             complement(8627..9274)
                     /locus_tag="VLE22_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB14773.1"
                     /translation="MTPMADNETKTAVSNWPIEVAVLDDDLDFLQYIEDFLKTEGQYS
                     VRSFSHPDDLAAACDQRTPDIVLIDMKMGEFRGENVIEEFSARHPGVCVIVVTGYPSL
                     ENMRTSFKLKVFDYITKPFSLAQMRQVLRNAADTFGLGRSPQDRLRGRLGHRIKMLRV
                     ERDWSLKDLSASTRLSVSQISSIERGAHLPSIESFLALCRAFDKRPSEVLASIDF"
     gene            complement(9271..10413)
                     /locus_tag="VLE22_09955"
     CDS             complement(9271..10413)
                     /locus_tag="VLE22_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB14774.1"
                     /translation="MKGLGTLFGSLRVRQKLIVIHNAFFALLAIAVVRFLHPTLEGWV
                     ALGVVYIVAVITLEVLIMPRYVYRPLQLILEADEATRRGDREAELVDEEQIPGDEIGI
                     IVRSRNATVAELRKHEDVLARMLTRVEHLADDLRRKNQLLETAKRKLEDQDRLASLGV
                     LSAGIAHELNTPLAVLQGSIEQLFESMPAGPTGERLQRLMRVTRRLRSVSESLLDFAR
                     APRQEMNAVSLRPAVDEAWSLVALDERAARVRFENRLGDGDVVHGSMDRLIQVFVNLL
                     RNSLYAVTDSGSITVCSMRSSVEGRDWITVSVEDDGTGIPDGVLPEIFEAFVTSRLDA
                     RGTGLGLTVAEGIVYLHGGTIEASNRPNGGARIDVRLPATPSEVLA"
     gene            complement(10659..13175)
                     /locus_tag="VLE22_09960"
     CDS             complement(10659..13175)
                     /locus_tag="VLE22_09960"
                     /inference="COORDINATES: protein motif:HMM:NF027656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HSB14775.1"
                     /translation="MPARIILVLILSLAAGCGGSKFRWTEGIPVAEQEPKPQDLLAYP
                     TDGQTAGVNPPGFFWTPRDKAAQYRLEVRKAGARSSAVLATAPQKSTVYPPFERLEPG
                     GYEWQVVYLDAGGKPTGLSKTRRFRLPAEAPVLLMPSLAEIKKKLAGTHPRVFLTNDR
                     LTRIRQAVARGTLPSWQQLQSAADAALEEKSYPEPEPYRPGVPSSEEWLRTFTPGKVG
                     SAHAARTALMYKITGDVRYLEGARRWMMTLASWDPKGITSHGLKLPGGDVGNDEASMP
                     MLERMSLAWDWAGAKLTPEERAKFLDSMTERGNQVLRILEKQDFLSHPYNNHSGRAIA
                     FLGTAGLAFLGDIPEAEKWLDYVVRAYLTSYPSWGGDDGGWSQGLSYWSFYIYCHANF
                     AEAFREATGVDLFQKPFFRNTGYFAMYFQPPYSPRGAFADGGYHPPNESGGLLAGTLA
                     DMLGDPVLKWYSLEVAEMGEKNETKWREWFTEDVYATLAAGRARRVEPKAPAGLEGVR
                     LSADIGWVAMHSALGDPENDVWVLFRSGRFGSYSHSHSDQNTFQINAYRRALAIDSGY
                     YPSFGTPHHVLYTRQTLAHNGILVNGRGQPAFEWKASGEIEQFDEQGPITFVRGEAGS
                     AYNIPQPPSVLEQWTKNLKAPIPPMEPAVETFERTLAFVESKTRPVLVVHDYLRTASP
                     TTFDWLLHALNKMETGAGAILLRDGDARLAVRLIATVPYSFSQRSGFPIKPEFAANTA
                     YILGKESFADQWRLNATTGKPAAEVKFMAIMVPYRASQPEPKIEPLAAGTGFRVGDTE
                     VAAWWGDGFRGKIQAGDLSGDGRLAVRVVENGRPRTAIAQ"
     gene            complement(13194..15044)
                     /locus_tag="VLE22_09965"
     CDS             complement(13194..15044)
                     /locus_tag="VLE22_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB14776.1"
                     /translation="MIALVLLVFLSISAPAATLRVGPSGQYAAPCAALAAANDGDVIE
                     IDAPGVYRGDTCAIYRNNLTIRGVNGRPKIDAGDRNTEGKGIWVVKGNNTTIENTEFT
                     GAAVPDRNGAAIRQEGANLTVRNCWFHHNENGILTGGSAGSEILIEYSEFGDNGYGDG
                     YSHNMYINHVAKFTLRFSYSHHSREGHLVKSRAAENHILYNRLSDEATGTGSYEIDLP
                     NGGKSYIIGNVIQQGPETHNSVLLNYRGEGPHPDNPSTELFVINNTFVNERTGGSSTF
                     IRIDPSVTTPGVIRNNIFAGPGTVNTQAGSVFSENITGADLRFLDPANYEYRLRAGSP
                     AVDRGADPGAPLTPGFAYVHPACGEGRQTVNSIDAGAFESGGGAFNADAPARCRKVDA
                     GGVVNAADFSSGPVAPGSIVSIFGSNLASEAVQAEKVPLPVDLRGTSVTVNGVAAPLF
                     YASPSQINAQVPFETQPGLAAVTVNASGIHTEAATVEVVASAPVIFVWSEGRAIVQNQ
                     DLTLNTPQNPAASGSVITAYLTGQGSVTPRVPTGGTAPEQPPLAIPDAPYSAIIGGQS
                     ARVGFLGLTPQLVGLLQANITVPMMSSPGDYPLIVIIGGAAARPAAVSIK"
     gene            complement(15041..>15233)
                     /locus_tag="VLE22_09970"
     CDS             complement(15041..>15233)
                     /locus_tag="VLE22_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HSB14777.1"
                     /translation="QELFVEGLVPIETLPGGRYGYQENTRKIVAERSRKAYSIGDTVK
                     VRLDRVDAVERKLQFSVVK"
BASE COUNT         2784 a         4572 c         4980 g         2897 t
ORIGIN      
        1 gacctgcctc gtgccgttcg cctcgacacg gctacgcgca gccgcaggtt tcggcggaga
       61 agaccataca ccgggcacgt tcggctttct gacgtggggt ggtgatggcg gcgccacggc
      121 ggcggagaac acgctgctca cagtgctcgg cgggtgcttc tgcgcgggtt gtgaggccgc
      181 agcccgtaag gatggtcttg atctcaaggc ggtgcgccgg gcgctcaggc cactcgcgga
      241 catgctcgat cacgccggtc ccgcggaaac acaccgattg gcgctgctgc gcgcgtcgaa
      301 tactaccccc accgcgctcc tcttgcggca tcccgagctg tttgagtgga tgaagttccg
      361 ctgtacgtcg atcacccggc tcttcgcgga cgcacacgcg gctgtgcgcg cgatcaaccc
      421 gaagatcgac ctgcggctga acgcgtacat cagccgaaac ccggaactca gcggaatcga
      481 ccttgcttcc ctgaaacacc acctgggatc ggtgcgttcc agtgactact cggagcagtc
      541 cggaatggca tcacagttgg agcacaagcg gcagttcctg ctgagcatcc gtgaggcagt
      601 gggcgacgag atgcacttcc tgtcggcgat cggcatccgt ccgaaggcga cacccgaact
      661 gatccgccag ggtgtccaga tttcgtcgga gtgcggagcc gacgggctct cactcgggca
      721 ctacgatggc gcccccctgc ggaacctgga tgcaatccgc gagggcatgg cggcagcgga
      781 cgtggtgatg cgagcgggga ggatacacaa ggcacttttg tagtactctt gcttgcatgg
      841 gcgagccagg cgaatgccgg tttgctaagt cgacgagatc cgggagaaaa cgaaccatgc
      901 gtgttgtgaa gatattcgca cccctcgtgt gccttttcag cctgttagcg ctgccgctgg
      961 cggcgcagtg ggaagagtcg ggcgacgtgc cgtacgtacc gacgcccccc gaggttgtgg
     1021 aggcgatgct gaagcttggg agcgtcaaga aaaccgacgt cgtttacgac ctcggctgcg
     1081 gcgacggacg cattgtcgta atggcggcgc agaagtttgg cgcgcgagcg gttggggtgg
     1141 atatcaaccc ggagcgcatc aaggaagccc aggcaaacgc gaaagaggcc ggcgtcaccg
     1201 ggcgcgtgga gttccggcag ggcagcttct ttgacgccga catcaaggat gcgaccgttg
     1261 tcactttgta tttgttgtcg agcatcaatc tgaagttgcg accgaagctc ctgaaggagt
     1321 tgaagccggg cacgcggatc gtttcgcaca gcttcgacat gggcgactgg aaacccgaca
     1381 aggagatcga agtcagcggg cggagaattt acctctggca cgttccggcc aacgcgggct
     1441 ctcactagcc ctatgactgc ggactcaggg ttctgcacgc tccgcattgt cattgccgga
     1501 gcggcggcga tcatcgtttc agcctgcttt gggcagaagc cggatgctca gccgagagaa
     1561 atcaacccgg gtgggcccgg aaaagcgcct tccgatgcgg tggtcctgtt cgacggcaag
     1621 gacatgtccc actggaccac tcgcgacgga aaaccggcac ggtgccaggt tcagagcgga
     1681 gatatggtct gcctgacggg cgtcggggac atctacagcc gggagaagtt ccgcagcgcg
     1741 cagatccatc tggaattcgc cgtccctttc atgccggagc agaaagatca aatgcgcggg
     1801 aacagcgggg tgtatctgca cggcaagtat gaaatccaga ttctcgattc ctaccacaac
     1861 ccaacgtatg cggttggcgc ttgcggcgcg ctttacggcc agtcgccgcc gctggtgaac
     1921 gcgagccggc caccggggca gtggcagtct tacgacatcg tgttccatgc cccacggtgc
     1981 gatggagccg gaaagatgat gtctccagcc acggtcaccg tgctccaaaa tggagtgcta
     2041 gtgcaagacc acgtgaagct cgagaacagt acaggctgcg gcgaggaagg gccgctgatg
     2101 ctacaggatc acagcggatt tcccggcgcg ccacagacgt ctatgaaatt ccgaaacatc
     2161 tggctccggc ggctcgatga gtaaaggcac gggctggagc ccgtgcctcg tgtgagttac
     2221 tcgcggggaa tcttgatttt cgtcgttttg ccggccacaa tctcgatggt ctcattgatc
     2281 tcgccgtaca tgggtgacga gcaacgaagc tcgtattttc cagggggcag caacagaccc
     2341 gtgccgacat ggttcatctc ttcaaggtgc ccgacgaagc ggccattcag gtagacggcg
     2401 gaagtatccc caacaaccat cgacatgaac gtatccggct tgcctcgccc gaatttcaga
     2461 gtgccgaagg gtcccttcgg ttcgggcagc ttcgtgagtt tgtacttgag gtcggtgacc
     2521 tcaccggcct tgacggtcac tgtggtcttg aaatcctcgt agcggggatc ctgaagaacg
     2581 acttcatggg tcccttcttc gacctgatac ttcaccgcga acgagtagcg gacggcgggt
     2641 ccgaggtact tgccatccac gaacaagccg gcgtcaccgg gttttccgtg ggcctttaaa
     2701 gtgccagtcg cggctgtggc cggcaggacc aaggccagac aaaaaactgc cagaatgatc
     2761 tgcttcatct attttgtttt cctccacatc tgacgttttg gattacagcg gatcgccatt
     2821 acctttacta tgatgccatt agacgcacgc cggttgcacc aacatacatc ctgccgccag
     2881 atgctatcgc aggttctcga tcagaccata gatcggccgc agcgttggat agagcgtccg
     2941 gtagacctcg tgaccgcgac ggtagagctg agactcgtgc ggcctcggca ggaccgactc
     3001 gacctcgcgg atgactgccc ggcaggcttc gggcaccgag ctgtaagcgc ctgtacctgt
     3061 catcgcgagg atcgccgcgc cataggccga gccttcctgt gaggctagcg ttaccactcg
     3121 tttaccgaag gtgtcagcca acaactgccg ccagaacggg ctccgtgcgc cgccgcccga
     3181 agcgcggacg gactccacgg ctacgccaca ttgctcgatg acgtcaaggc agtccttctg
     3241 gctgtaagag actccttcga gtagcgagcg aatcaggtcg gcgcgggtgt gtttcgcggt
     3301 gataccgatc cagccgccgc gagcggtgga atcgaggtgc ggcgtgcgtt cgcccatcat
     3361 gtacggcagc cagaacagcc catgggaacc ggcgcccgcc ccggcggctt cggccgtgag
     3421 cgcgtcgtac tcggcgcccg gtgcgaactg gttgcggaac cactggaggc tcagacccgc
     3481 gccctgtgtg acgcccatga cgtgccaagc gcccttgacg gcatgacaga aggtgtggac
     3541 acggccctgc gggtcgtagg tggccttctc catgtgcgcg aagacgacgc cagaggtgcc
     3601 gagcgtgcag gacacgatgc ctggttcgac gataccgttg ccgatggcgc tcgacgcctg
     3661 gtcgccgccg ccacccgcca cgggcgtacc ctcgatcagg ccggtaaggg ctgccgcctc
     3721 ggctgtgacc tggccggtga tctcactcga ttcgtaaacc ttcggcagga tcgcgcggtc
     3781 gacgccgagc cggtcggcca tctggttcga ccaacgacgc attacgacgt cgaacagagc
     3841 tgtgccggag gcatcggaca cctcggtagc gtactcaccc gtgagctgga agcggacgta
     3901 atccttcggc aggagcatct tgcggacgcg cgcaaaagct gccggttcgt tgtcgcgaac
     3961 ccacagcagc ttaggcaggg tgaagccggt cagcaccggg ttggcggtaa atgcaacgac
     4021 gttactccgg ccgatgcgct gattgatgaa gtccacctgc gcttggctac gctggtcgca
     4081 ccagatgagg gccggacgga tgacggcgtt actttcgtca agcagcacca ggccgtgcat
     4141 ctggccggac aggcccacac ccttgatgtt cttgcctgag accttggcct cagccatcac
     4201 gccttggatc gccttccggg cggcgtccca ccagtcttcc gggcgctgct ccgcccagag
     4261 cggtttctcc atgcgcatat cttcgtgcgg cgcggtgaac ccggccttca ccttgccctt
     4321 ctcgtccacc aggagcgcgc ggcttccgcc ggtaccgatg tcgatgccca accagtacat
     4381 agatgaagtc ctcatgattg cttactttta caagcttgat ctttcgcctg gaacggaagg
     4441 taggccgcgc cgaacagtcc cgcttcgttg ccgaggatgg ccttctcgat ccgggttcgg
     4501 gttttcttat acgtgaacga acgcgtctcc agctcgcgaa acatcgtagg tgcaaagtag
     4561 tcccacgccg gtaagacccc tccgctcagc agatacaggg gaaaattaaa gatattgacg
     4621 aggtttgcca gagcgattcc gagggcgctt cccatgctct cgaacacccg ctgcgcgctc
     4681 tcattgccct ccaccgcaag ccggtagaca ccctcggagg tgatgttgtc gccgaggccg
     4741 agcatccgtg cggtggcgac gacagccgtg gccgaggcgt gtttttcgag gcagccacgg
     4801 ttcccgcaac cgcatgggtt gcctgccgga ttcacggtga tgtggccgac ttccccagcc
     4861 atacccacga agccgtggag cacctttccg cccgatatga cacctccgcc aatgccagtc
     4921 cccagggtca gcaagaccag gtcattcaca ccccttccgg ctcccatcca cttctcgccg
     4981 agggcggcgg cgttggcgtc gttctcgagt atgaccgggg cgtgcagccg gctctcgatt
     5041 tcgtccctga ccgggaagtc ctcgaagccg ggaaggttcg gggatccgac gacgagtccc
     5101 ttttcgatga caatgaaccc agggaccccg atgccggcgc cgaccaaacc gtccgcgccg
     5161 agccggtcgc gaagcttaac gatggaggcg acgatgtcgt caatgacggc ctcgcggccg
     5221 gccgagagct ttgttccgcc agaggttttc cggagcacat tgccgtcccg gtcaatggct
     5281 gcggcgcgca gattggtgcc gccgaggtcc actccaatcg aaaattcggc catgagatgg
     5341 catcataaca gatacgccgg tacggattag cggcgggcac ttctggaggt tccatgggca
     5401 cggtgcatgt ggtttttggc gcggcgggtg gtctcggggc cgccattgtt cgaaagctga
     5461 ccgcgggcgg tgaaccggtt cgcgccgtgg taagggatgc aggaaaagcg aagcggttgt
     5521 ttgccgagcc ggtggaggtt gtggccgcgg acgcacggga cgccctgagc acccggcttg
     5581 cctgccgggg cgcatccgtg gtctaccact gcgtcaacgt gccgtacaac ctctggcggg
     5641 aggtgctgcc ggcggtcacg gacaacattc tggaaggggc gcggtacgag gaggcccggc
     5701 tggtgttctc cgggaacgtc tacgggtacg gctacttcga caaacttccg gtaaccgaga
     5761 atcatccgcg ggcggcgctc actcgcaaag gtagtcttcg caatgaattg gaagacaaac
     5821 tgcttgaggc gcaccaggca ggcgacgtac aagttgtgat cccgcgaatg ccggacttct
     5881 atggcccgaa cgtgaccaac aagctgtacg ggggcttgtt cgaggctgcg cttgcgggca
     5941 agcgcgccac gtggctcgga aatgcagagc tgccgcacga cctggtgttt atcgacgacg
     6001 cggcggctgc ctgtgtcttg ctggcggcaa ccggcagcgc atacgggcag gcgtggcaca
     6061 tcaccagccc agaaaccctg accggccgcg agttcctgcg cctgatttac cggacggcgg
     6121 gtgcgtcttt gagggtgttc gtggctgacc gcttcctgct ccggctcggc gggctgttcg
     6181 acccggtggc tcgggagctc atcgaaatcc tgtatctgtt cgagcaaccc caagtgctcg
     6241 acgggacgaa gttccagcaa gccttcccgg aattccggta cacgccgcac gcggaggcgg
     6301 tgcggaggac gctggagtgg ttccgggggc ggacgaccga cagatccggc cactgacgtt
     6361 cgcggttcct tcagcgcact agggtttcgg cggctgctcg gtggtcttct ccgctggcag
     6421 cggctcgggc gtctcaaagg tgacggtcgc ctcggcggcg aacttgcggt agaggcgaaa
     6481 ctcaacttcg ttcttgacga ggaacttgtt ctcccgcatg cggaccacgg ccttcagagg
     6541 cagcatgtgc tgctggccgg cgatctcggt gtagtcgtag tcgaggaccg aagaagcctg
     6601 ctggatcgga aagtccgccg ggatgtcctc ggcttcgagg gtgatccgca tgaccatttc
     6661 cgtgtctcga tcgacgtaga tcagcccctg gtaggcggga gtgacgtcga ggcgacgctc
     6721 gtagtcgatc cgccatttcg actttggctt cgagacgcgg tacgaaaaca catgggcccg
     6781 tttgccgcga agcgtggccc accgctccca gcggaaccgc gcctgcgtct ccggttcgaa
     6841 gatctcgcgc atcatgctgc cgaactcgcc ggtcgacgtg gcgccgccaa tggactcgta
     6901 cggacggtcg gtcgtcacac cgttgatgag gatgaccttg tagtcctctt tttgctcgaa
     6961 gtaggtgagc cgggcagtga tcacgtcggc gcgctgccag aactcgaggc cggtggggtc
     7021 gatgtaacgt cgcgtcacct gggtgcagat gaaatccgga agctgcttga cgtacccgag
     7081 cgcgtactca cggactttat ccatgatctg ccgttgttcc tcgatactcg gcggaggaat
     7141 ggacggcttc tccggctcgg gtggcttgcg cgcgggcgcc ggcaaggcct tggacgcacg
     7201 ctgaagctcg cggagcgctt cgatcgtttt cgggccggcc ccggccgcct gcaactcgac
     7261 gacctcccgc tcgtcgaggc tttgggtgag tttgaccgtt cggaggtact cggcgacgtc
     7321 tttgtccttg tgcttcagtt cgacggacga acgaacgaaa gagataagtt ggtcgacggt
     7381 gagccgggct tgggcccaga ctccaagcgt tgccgcaaca agcaacagcg ccttggccgc
     7441 gttcatactt ctattctcca ccgaagaagg ggaagcgacg cctcttgggc ttcttgccgg
     7501 cggcggcctt gatctccttg tcgatggcgg cgagcgcgac cggggccatc agccggtagc
     7561 ctttgccgtt tgggtgcagg ccgtcgtcgg aggaatcctc cttgagcagc ccagaggtat
     7621 ccgcgagctg gctgaaatag tcgacatagg tgtagccacg ctgatggcag aacccctcga
     7681 tccatgcgtt gagctgccgg atggtggcgg gcgggcggcg cttggtctgc tcgtagcgcg
     7741 ggtcgtcgcc tttatggtaa tcgctgaccg gaagcagaga agcgaacagc gggcgtatac
     7801 ccgcggcttc ggcgaggtcg gcgatcatgg tgaggttgtt ctggatcgtc ttgacgtcga
     7861 cgcctcgcgc aatgtcgttg gtgccggcca gcacgagcag gtacgaaggc ttgaggtcga
     7921 tgacatcggc tctcatccgg ccgagcatct cgccggtgat ctgcccgctg atgccccggt
     7981 tgacgaagtc gcggtccggg aagtactcgt tgagacgcca cccgtcggtg atcgaatcgc
     8041 ccaggaacac caccctcggc aggtcgggtt tcggcgccgg cagcttaccg tttgcggcgg
     8101 cgtagcgccc gaggttgtcg cggtcggagt tccgcagttg cgactccttg agttcgagca
     8161 acgcccggaa gtgtgactcg gcgcggtcgg cggtatcgcg cagttccgcg aactggcggc
     8221 gagcctcgtc ggggaacggg aacggctttg ggatcgaatc ggagagcgca aggtaggcgc
     8281 gaaggttggc gaggaacgtg taggtgacgc cggcgttctg cgcgccggtg gctttcagtg
     8341 tcaccaccgc ctgacgtacg ttctcagata ccggcgcacc cgcgcgggcg agctcgggaa
     8401 cggccaccct ggtggaatcg agcagttgca cggcacggtc gaacagtttg agagcgtcgg
     8461 ggtttgacag cagtgcgttc tgctgggtcc acgacgccgg agcggaaagc agaagcgcaa
     8521 tccaaatggt gaacgagcgg aatctcatcg ggtctccaaa acccgagttt gacagatgcg
     8581 aaaaagcggg cgcaagagcg gaccagcgcg gcacgttcac gcgcgatcaa aaatcgatcg
     8641 atgcgagaac ttcggacggc cgcttatcga aagcacggca gagcgccagg aagctttcga
     8701 tgctcggcag atgcgcgcca cgttcgatgg agctgatttg cgagacgctc aggcgcgtgg
     8761 acgcagagag gtctttcaag gaccagtccc gctccacacg gagcattttg attcggtggc
     8821 cgagacggcc tcgcaggcgg tcctgggggg accggccaag accgaaagtg tcggcggcat
     8881 tccgcagcac ctgccgcatc tgcgccagcg agaacggctt ggtgatgtaa tcgaagacct
     8941 tgagcttgaa gctggtccgc atgttctcga gcgacggata gccggtgacg acgatcacac
     9001 agacgcccgg atgacgcgca ctaaactcct cgataacatt ctcgccgcgg aactcaccca
     9061 tcttcatgtc gatcagcacg atatcgggcg tccgctggtc gcaggccgca gccaagtcgt
     9121 ctgggtggga gaagcttcgg acggagtact gcccctcggt tttgaggaag tcttcaatat
     9181 attgaaggaa atccaagtcg tcgtcgagaa cggcgacctc tatcggccag ttggaaacgg
     9241 ccgttttcgt ctcgttgtcg gccatcggtg tcatgcgagt acctcgcttg gagttgcggg
     9301 gagccgcacg tcgatgcggg ctccaccgtt cgggcggtta gaagcctcaa tcgtcccccc
     9361 atgcaggtag acgatcccct cggccacggt caggccgaga ccagtgccac gcgcgtcgag
     9421 ccggctggtg acgaatgcct cgaagatctc gggcaggaca ccatccggga tccctgtgcc
     9481 gtcatcctcg acgctgacgg taatccagtc cctgccctcg acgctggaac gcatcgagca
     9541 gacggtgatg ctcccggaat cagttacggc gtacaaagag ttgcgaagaa ggttaacgaa
     9601 gacctggatg agccggtcca tgctgccgtg gacgacgtct ccatctccaa ggcggttctc
     9661 gaacctcact ctggcggctc tctcgtcaag ggcgaccagg gaccaggcct cgtcgacggc
     9721 gggccgcagc gacaccgcgt tcatctcctg gcgaggcgcc ctggcgaagt cgagcaggct
     9781 ctcgctgacg ctgcgcagcc ggcgggtcac ccgcattagg cgttgcagcc gctcgcccgt
     9841 ggggccggcc ggcatggatt cgaatagctg ctcgatagag ccctggagca ccgcgagggg
     9901 ggtgttcagc tcgtgcgcaa tccccgcgct cagcacgccg aggctggcca agcggtcctg
     9961 gtcctcgagc ttgcgcttgg ccgtctccag aagctggttc ttgcggcgaa gatcgtcagc
    10021 caagtgctcc acgcgagtca gcattctggc gagaacgtcc tcgtgcttgc gcagctcggc
    10081 gacggtggcg ttccgcgaac ggacgatgat gccgatctca tcaccgggga tctgttcctc
    10141 gtcgaccagc tcagcctcgc ggtcgccgcg ccgggtagcc tcgtccgctt cgaggatgag
    10201 ttgcagcggc cggtaaacgt aacggggcat gatcaggacc tccagggtaa tcacagccac
    10261 gatgtagacg acgcccagcg caacccagcc ttccagagtc gggtgcaaga aacgaacgac
    10321 cgcgatcgca agtaacgcaa agaatgcgtt atggatgacg atcagcttct gccggactcg
    10381 taagctgccg aaaagtgtcc ccagaccttt caacggctct cctgaatcga ccgtagatgg
    10441 catccggcgg cctcgaattg ccctcgccac ccgaatttcc cagtaccgac ttactgcttc
    10501 gtgctttgta gcataccact atttccggtt taagaacagc aattacagcg tccggcgtaa
    10561 tgagtcgcaa aaaatgcagc ttttctggca ggctctggcc tgcgagggtg gcagacgaca
    10621 aaagacgatc atctgccccc ccacaatact ggcgactgtt actgtgcgat ggcggtacgc
    10681 ggccggccgt tttcaacgac gcgaacggcc agcctgccat cgccggagag gtcgccggcc
    10741 tggatcttac cccggaaacc gtcgccccac caggcagcga cctcagtatc gccgactcgg
    10801 aagcccgtgc cagcggcgag aggctcgatc ttgggttcag gctgggaggc gcggtagggg
    10861 accatgattg ccatgaactt gacctcggcg gcgggctttc cggttgtggc gttcaggcgc
    10921 cactggtcgg caaagctctc cttgccgagg atgtaagcgg tattggccgc gaactcgggc
    10981 ttgatcggga agcccgagcg ctgggagaag ctgtagggca cggtagcgat gaggcgtacc
    11041 gcgagccggg cgtctccgtc gcgcagaagg atcgccccag cgccggtctc cattttattc
    11101 agggcgtgga gcagccagtc aaaggtcgta ggtgaagccg ttctcaggta gtcgtgaaca
    11161 acaagcacgg ggcgggtttt cgactcaacg aaagccagcg tgcgctcgaa ggtttcgacc
    11221 gccggctcca tgggcgggat gggcgccttg agattcttgg tccactgctc gagaacagac
    11281 ggcggctgag ggatgttgta ggcggatccg gcctcgcctc gcacgaacgt gatgggaccc
    11341 tgctcgtcga actgttcaat ctccccggac gccttccact cgaacgccgg ctgcccgcgc
    11401 ccattcacaa ggatcccgtt gtgcgcgagc gtctgccggg tatagagcac gtgatggggc
    11461 gtgccgaagg aggggtagta gccggaatcg atcgccagtg cgcggcggta cgcattgatt
    11521 tggaacgtgt tctggtccga gtggctatgg ctgtatgagc cgaagcggcc ggaacggaag
    11581 agcacccaga cgtcgttctc cgggtcgcca agggcggagt gcatcgccac ccatccgatg
    11641 tcggccgaga gccgcacgcc ttcgagaccc gcaggcgctt tcggctcgac ccgccgtgca
    11701 cggcctgccg cgagcgtggc atagacgtct tcggtgaacc actcgcgcca tttcgtctcg
    11761 ttcttctcgc ccatttcggc gacctccagc gagtaccact tcagaaccgg gtcgcctagc
    11821 atgtcggcga gggtaccggc gagcagcccg ccagactcgt taggcggatg gtagccaccg
    11881 tccgcaaaag cgccgcgtgg cgagtacggt ggctggaagt acatcgcgaa gtagccagtg
    11941 ttgcggaaga agggcttctg aaagagatcg acgccagtgg cttcccgaaa cgcttccgcg
    12001 aaattcgcat ggcagtaaat gtagaaggac caatagctta gcccttgcga ccatccgccg
    12061 tcatcgccgc cccaactggg gtacgacgtc aggtaggcgc ggactacata gtcaagccac
    12121 ttctcggctt ccggaatgtc tcccaggaaa gcgagaccgg cagtcccgag aaacgcgatg
    12181 gcgcggcccg agtggttgtt gtacgggtgc gacaggaagt cctgcttttc gagaatccgc
    12241 agaacctggt tgccgcgctc ggtcatggag tcgaggaact tcgcacgctc ctcgggtgtg
    12301 agcttggctc cggcccagtc ccaggcgagc gacatgcgtt cgagcatcgg catggaggct
    12361 tcgtcattgc cgacgtcgcc acccggcagc ttcagcccgt ggctggtgat accctttggg
    12421 tcccagctcg ccagcgtcat catccagcgt cgagcaccct cgagatatct cacgtcgccg
    12481 gtgatcttat acatgagcgc ggtccgggcc gcgtgcgcgc tgcccacttt gccgggcgtg
    12541 aacgtacgga gccactcttc gctggaagga acgccgggcc ggtacggctc cggctccggg
    12601 taggatttct cttcgagcgc ggcgtccgcg gcagactgta gctgctgcca ggagggcaac
    12661 gtcccgcggg ccaccgcctg ccggatgcgg gtcaggcggt cattcgtgag gaaaacgcgt
    12721 ggatgcgtgc cggccagctt cttcttgatt tcggcgaggg acggcatcag tagaacgggc
    12781 gcctcggcgg gcaggcggaa gcggcgtgtt ttcgacaggc cggtgggctt gccgcccgca
    12841 tcgaggtaaa caacctgcca ctcgtacccg ccgggttcga gccgctcgaa tggagggtat
    12901 acggtggact tttgcggtgc ggtcgcgagg acggcagacg atctggcgcc ggcttttcgc
    12961 acttcgagtc ggtactgcgc ggctttgtcg cgcggggtcc agaagaagcc cggcgggttc
    13021 acgccggcgg tctggccgtc ggtcgggtac gcaaggaggt cctgcggctt gggctcttgt
    13081 tccgcgaccg ggatgccttc ggtccatcgg aatttggagc cgccgcaacc ggcggcgagg
    13141 gacagtatga gtaccagaat gattcgtgcg ggcatgtcga tcaactctga atcttacttg
    13201 attgagacgg cggcggggcg ggcagcggcg ccgccgatga tgacgatcaa cgggtagtct
    13261 cccggtgagg acatcatcgg cacggtaatg ttggcctgaa gaagaccgac gagctggggc
    13321 gtgaggccga gaaaccccac gcgggccgac tgcccgccga tgatggcact gtaaggcgca
    13381 tccggaatgg ctaagggtgg ttgctccggc gcggtgccgc cggtcggaac gcgcggagtg
    13441 acggagccct gaccggtcag atacgccgtg atgacgctgc cagaagcggc cggattttgc
    13501 ggcgtgttca gagtcaagtc ctggttctgg acgatcgcgc ggccctcgct ccagacgaat
    13561 ataacgggcg cggatgccac gacctcgacc gtggcggctt ccgtatggat tccgcttgcg
    13621 ttgacagtga cggcggccaa gcccggctgc gtctcaaacg gtacctgggc gttgatctgc
    13681 gacgggctcg catagaaaag cggcgcggcg acgccgttga ccgtcaccga ggtccctcgc
    13741 aggtccacgg gtaacggaac tttctccgcc tgtacggcct cacttgccag gttggaaccg
    13801 aagatcgaga caatcgagcc gggagcgacg gggccggagg aaaagtccgc cgcgtttact
    13861 acgcctcccg cgtcgacctt gcggcaccgt gccggagcat cggcgttaaa cgcccctccg
    13921 ccagactcga acgctccagc gtcaatcgag ttgacggtct ggcggccctc gccgcaggct
    13981 ggatggacgt aggcgaatcc aggagtgagc ggcgcgccgg gatcggctcc tctgtcgacc
    14041 gccggcgaac cggcgcgcag tcggtactcg tagttcgctg ggtcgagaaa ccggaggtcg
    14101 gcgcctgtga tgttttccga aaagacgctg ccggcctgcg tgttgacggt gcccggcccg
    14161 gcgaagatat tgttccggat cacgccaggg gtagtgacgg aagggtcgat gcggataaag
    14221 gtgctcgacc ccccggttct ttcgttgacg aacgtattgt tgataacgaa gagttcggtg
    14281 gaagggttgt cggggtgtgg cccttcgccc ctgtagttca ggaggacgct attgtgcgtc
    14341 tccggtccct gttgaatcac gttaccgatg atgtacgact tgccgccgtt cggcagatcg
    14401 atctcgtagc ttccggtgcc ggtggcctcg tcgctcaggc ggttgtacag gatgtggttt
    14461 tcggcggcgc gcgactttac caggtggccc tcgcgcgagt ggtgcgagta ggagaaccgc
    14521 agggtgaact tcgctacgtg gttgatgtac atgttgtgcg agtagccgtc gccgtacccg
    14581 ttgtcgccaa actcggagta ctcgatcagg atctcgctgc ccgcggagcc accagtgaga
    14641 atgccgttct cattgtggtg aaaccagcag ttgcggaccg tcaggttggc accttcctgg
    14701 cggatggccg cgccattcct gtcggggacg gcggcgcccg taaactcggt gttctcgatc
    14761 gttgtgttgt tgcccttcac gacccagatg cccttgcctt cggtgttccg gtcacctgcg
    14821 tcaatctttg gccggccgtt gacgccgcgg atcgtgaggt tgttccggta gatggcgcag
    14881 gtatcgcccc ggtagacgcc aggcgcgtcg atctcgatca cgtctccgtc gtttgcggcc
    14941 gccagggcgg cgcagggagc ggcgtactgt ccagaagggc cgacacgcag cgtggcggcg
    15001 ggagcggaaa tcgaaagaaa tacgagaaga acgagcgcga tcattttaca accgagaact
    15061 ggagttttcg ttcgaccgcg tccacgcgat cgaggcgaac tttcacggtg tcgccgatgg
    15121 agtaagcctt gcgcgagcgc tcggcgacga tcttgcgggt gttttcctgg tagccgtagc
    15181 ggcctccggg cagcgtctcg atgggcacca ggccttcgac gaacagctcc tgc
//
LOCUS       DATIFQ010000211        10047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_645403, whole genome shotgun sequence.
ACCESSION   DATIFQ010000211 DATIFQ010000000
VERSION     DATIFQ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645403"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..206)
                     /locus_tag="VLE22_09975"
     tRNA            complement(133..206)
                     /locus_tag="VLE22_09975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(172..174),aa:Gln,seq:ttg)
     gene            307..1200
                     /locus_tag="VLE22_09980"
     CDS             307..1200
                     /locus_tag="VLE22_09980"
                     /inference="COORDINATES: protein motif:HMM:NF040125.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6528 family protein"
                     /protein_id="HSB14778.1"
                     /translation="MPLFFALLCILLSPGTAAAAGGGQLVVCGWDEVFVFDLGAPDNP
                     GGKIWSWRAKDRSELPAELYARFAATDECKPVDGGRRILITSSSDGVALVDYPSGKVV
                     FHATAPNAHSAELLPGGRIAVAASHRPNAPGDRLVLFDIKRPGKPLFHAELSWGHGTV
                     WDQKRQILWALADEDLHAYTLKDWDTDSPSLALSAKYRMPDRGGHDLYPAADTPALTV
                     TTGKHCWLFDRDTRHFSPHPELADKASVKCITVDSATHRTVWMQAEGANWWSERLRFL
                     NPTGELIIAGEKLYKARWMPQ"
     gene            complement(1221..1685)
                     /locus_tag="VLE22_09985"
     CDS             complement(1221..1685)
                     /locus_tag="VLE22_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14779.1"
                     /translation="MTSRIGTVLCLAMAGWLVSCGSPAVEGGGPAVNDITTEEVRASG
                     AGGCGMTLRRVEGSGSGQFVFFHGIAPDSGRMRLDGLMLRLRKEQGSGDPFFGQEPAQ
                     RFRTPEGRIEVEVEVSPGEKDNRGVIPIKSGTIRVRKDGRETLVSVTGEAGC"
     gene            complement(1719..2129)
                     /locus_tag="VLE22_09990"
     CDS             complement(1719..2129)
                     /locus_tag="VLE22_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSB14780.1"
                     /translation="MGRRIPTGNPRRGFTLVELMIVMTIVTILVSIAVPLYQRSILRA
                     KESVLKNNLFTMRTVIDEYTLDKQKAPQTLQDLITEGYLREIPMDPITGSGETWRVIM
                     EDATASVDQTEPGIFDVRSGSDKVSLDGTPYAEW"
     gene            complement(2104..2568)
                     /locus_tag="VLE22_09995"
     CDS             complement(2104..2568)
                     /locus_tag="VLE22_09995"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSB14781.1"
                     /translation="MRRKNQSGLTLVELIVAFTIMSLLTAMAVPVARYKVRREREKEL
                     RYALREVRSAIDRYKDYSDQGLLGPPKVDAEGYPENLQVLVEGVKLSGTVDKKVRFLR
                     RIPKDPLTDSYEWGMRSMQDEPDSKSWGGQNVFDIYTKSTEKAADGTPYSDW"
     gene            complement(2576..5068)
                     /locus_tag="VLE22_10000"
     CDS             complement(2576..5068)
                     /locus_tag="VLE22_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HSB14782.1"
                     /translation="MRIQNRFVLLLAAVGLLLVGALTGLAGNKKGDRFLAQGRDAEVR
                     NDFDKALGFYEQALAEDPADVAYQMATKRMRFQAAQMHVNQGQKLRTEGQLEEALAEF
                     ERAHAIDSSSAIAGQELRRTLDLVERERKRKAQPGEKGGEQGAGTLEEAQREIEERLA
                     TVQSVPELKPLSRTPITLKMNNQPPKVLFETVGKLAGINVLFDPDFTTQVQQKALNLE
                     VVNATLEEALDYVGVMTRSYWKPLSSNAVFVTADNQQKRAEYEDTVAKVFYLTNVTTS
                     QELNEISSTIRTVANAQRVFIFNSQSAIIVRGTADQVALAELIIHNLDRPRPEVVVDV
                     IVMEVGRTRSRDLSATIASGGAAGINVPISFTPRNPRLSGGTGTGTGTGTGTGTGTGT
                     GTGTGTGTGTGTGTGTGTTPQSTAISLAQIGKISTNDYSLTMPGALLEAVMSDRGTKV
                     LQSPQVRAVDGQKASLKIGDRVPYSTGGFQPAFGQVGTGFNSLYSSFQFLDVGVNVDL
                     TPKVHGLDEVSLHIELDISNVRDRIDIGGVSQPVVGQRKVVHDIRLKEGEVSLLGGLM
                     QVQETKAVSGVPGLSSIPVIRRLFTSESIEKNESELLIALIPHVVRTADLRKENLKGI
                     ATGSERIVKLSFAPRPEAAPAVEAPKPAPAPEAPVPGVPKPEAPKPEATKPEPAAAKP
                     EAPAGPPAPGTPALVLFSPAAVEAKPGSTVTLTLQVENVTDLFSAPLRLKFDPAILRL
                     NEIVRGNLLAGDGKEVIFTRNILNDTGDATVNLSRLPGSGGVSGSGTLLTLTFQVVGR
                     GTATVTAPEFTPQNSQMQPLMTASPQVTVTVK"
     gene            5182..5643
                     /gene="smpB"
                     /locus_tag="VLE22_10005"
     CDS             5182..5643
                     /gene="smpB"
                     /locus_tag="VLE22_10005"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HSB14783.1"
                     /translation="MEDRIKILSDNRHAGHLYHLFERFEAGLALTGTEVKSARAGKVQ
                     LKDSYGEVLGDEAWLVNAHISHYSHGNRENHDPVRRRKLLLHRDEINKLHSKTREKGL
                     TLIPTKVYLKKGRIKCEIALAKGKKLHDKRETERKRTAEAEARAALRQRSL"
     gene            5661..7157
                     /locus_tag="VLE22_10010"
     CDS             5661..7157
                     /locus_tag="VLE22_10010"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HSB14784.1"
                     /translation="MEIQLLSQPIENIEANALVVVAFDKESPGEGLGPAAKRADEMAG
                     GWIREAYSTGEFSGKLLETSLFHRPQGLKASRLLLVGGGKAEKFTTAELRKLAGAVVR
                     ILKSKGSKDIAFALDGSTATASHVAATVEGALLGDFEPDRYKTEKKDQKTIARFSLAV
                     PAVTEELEESLRRGRIIAESQNFTRDLVSEPANRLNPSILAERARLMAADYGLEYEVL
                     DADRMRQVGMGALLGVAQGSSEPPALIILRYRPEGADHAAAHLGLVGKGVTFDTGGIS
                     IKPSEGMEKMKYDMAGGAAVLGAMRALAQLKPAIPVTALVPAVENMPGGRAQRPGDIV
                     TTLSGKTVEVLNTDAEGRLILADAITYARRLGCTHLVDAATLTGAIVVALGHVNVGAF
                     STDDAFLGKVLDAAKNGGEKLWQMPLDDDYKESLKSAFADIPNIGNRWGGAITAAMFL
                     KEFAEETPWVHLDIAGTAWLEEAKPFLAKGPSGVPLRTLVNLALSWRG"
     gene            complement(7129..7863)
                     /locus_tag="VLE22_10015"
     CDS             complement(7129..7863)
                     /locus_tag="VLE22_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB14785.1"
                     /translation="MVRIALYTDEPVSACGFMSLLLPAGGFELVSIWNDPASLPATVL
                     RAQPDVLVIDCNDHVALALVADIRRTAPACKIVLWARSIPAELAYQAIELGVRAILRK
                     TLDAAQMSGRLRDVAAGEVCLDGTLAAGWPPVETVRLTNRESQLLELLAFGMKNREIA
                     SALSITEGTVKVYLSRLFQKAGVRDRYELGIYGVRNLRGVTPVEVDGNGRSDSPTIPR
                     RGGRATPWPRSLALGRNFSPANSTQD"
     gene            complement(8022..8975)
                     /locus_tag="VLE22_10020"
     CDS             complement(8022..8975)
                     /locus_tag="VLE22_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HSB14786.1"
                     /translation="MGEIRFESAAVIGTGMMGPGIAVTLALGGLRVAIVSRTQDGAVA
                     GLEKASEQVRLLEENALLTSDRVEQLRDTLSATANFDETVAGADLVVESGPENMEFKQ
                     ELFKRMESLVPPSSVLASNTSSLSITAVASKCRHPERVLTTHFWNPPHLMPLVEIVKG
                     EQTPDEVARAVRDLLAACGKVPVMVRKDRPGQLGNRLQMALWREAVNIVQEGIADAAD
                     VDLAAKLGFGIRLPVYGIFEHADAVGLDMVHGIMDYTAKDLYNQPQAPPLMKELVARR
                     DLGVKTGRGFYDWSKKDIKAVLKLRDRFVLEFLKSEFGHSR"
     gene            complement(9053..>10047)
                     /locus_tag="VLE22_10025"
     CDS             complement(9053..>10047)
                     /locus_tag="VLE22_10025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB14787.1"
                     /translation="FPYAAGFLLWSVMSALAALAGSLWQLFALRMLLGVFESIVTPAG
                     MRWIRFHCAENQRGLAVGLYMAAAKAGPALGAPLATWLLVAYGWRPMFVILGLGCLVW
                     LLPWMLLVEDDDRKIERAAVQNAAASVPFLRVLASPVIWGTIIGTFCYQYFVYFCMTW
                     MPAYFVERRNLSLESMGWYTMFSFMGMAVVATAAGWAADRMIDRGGDPVRVRKAFTIA
                     GFLVASTEIFGALSDSHSVALFFAVFSLSGLGLTTANYWALTQTLIPGAAVGRIVGIQ
                     NCAANLPGIVAPLLTGWLKQTTGSYEAPMQAIWIFLLSGVASYVFLVRQKYAPR"
BASE COUNT         1892 a         3267 c         3056 g         1832 t
ORIGIN      
        1 gagggatgct cgtcttgcgt gacgcattct agccgtaccg ttgccagatc gggactggcc
       61 acctatcggc ggccagttcg agtgggggac tgggggcgcc cgcaaaacac aagattaaaa
      121 gggcgatttt gctggctccc cggcatggat tcgaaccacg attcacggct ccaaaggccg
      181 ctgtcctacc attagacgac cggggagttc ggttggcgct gcgtctacac cctagtctag
      241 cgtagcgtcc gaaagccgcg ctagtcgcgg cgacggcgtt cccgctacac tacaaaggct
      301 atgatcgtgc cgctcttctt cgctctgctt tgcatcctgc tgtcgcccgg cactgcggcc
      361 gcggccggcg gcggccagct tgtcgtctgc ggctgggacg aggtctttgt cttcgacctc
      421 ggcgccccgg ataaccccgg cggcaaaatc tggtcctggc gcgccaagga ccggtccgaa
      481 cttcccgccg agctttacgc gcgcttcgcc gccaccgacg aatgcaaacc ggtcgacggc
      541 ggacgccgca tcctcatcac ctcttcctcg gacggcgtcg cgctggtcga ctacccctcc
      601 ggcaaggtcg tcttccatgc caccgcgccc aacgcccact cagccgaact gctccccggc
      661 ggccgcatcg ccgtcgccgc ctcccaccgc cccaacgccc ccggcgaccg cctcgtcctg
      721 ttcgacatca aacgcccggg caagccgctc ttccacgccg aactgtcctg gggccacggc
      781 accgtctggg atcaaaagcg acagatcctc tgggcgctcg ccgacgagga cctacacgcc
      841 tacacactca aagactggga tacggactcg ccatcgctgg cgctaagcgc caaataccgg
      901 atgcccgacc gcggcggcca tgacctttac cccgccgcgg atacgcccgc cctcaccgtc
      961 accaccggga agcactgttg gcttttcgac cgcgatacgc gccacttctc gccccatccg
     1021 gaactggctg acaaggcgtc cgtcaagtgc atcaccgtag actccgccac gcaccgcacc
     1081 gtgtggatgc aggccgaggg cgccaactgg tggagcgagc ggctgcggtt cttgaacccg
     1141 acaggggaac tgatcatagc cggcgagaag ctctacaagg cgcgctggat gccacagtag
     1201 gctggcccgc ccgcccccgc tcaacatccg gcttcgccgg tcacggagac cagtgtttcc
     1261 cggccgtctt tcctgactcg gatcgtgccg gacttgatcg ggatcacccc tcggttgtcc
     1321 ttctcgcccg gggacacttc gacctcaacc tcaatccgtc cttccggcgt ccggaatcgc
     1381 tgcgccggct cctgcccaaa gaagggatcg cccgaccctt gctccttcct aagccgcagc
     1441 atcagcccgt ccaaacgcat ccggccggaa tccggcgcga ttccatggaa gaatacgaac
     1501 tgcccggagc cggagccttc cacgcgccgc agcgtcatgc cgcagccgcc ggcgccgctc
     1561 gcgcgcacct cttccgtcgt gatgtcgttg accgccggac ctcccccctc caccgccggc
     1621 gaaccgcacg acacaagcca ccccgccatt gccaaacaca gaaccgttcc aatcctcgat
     1681 gtcatgcacc tgacctccgc gccccgccgg tggaaccctc accactccgc atacggagtg
     1741 ccgtccaggc tgaccttgtc ggaaccgctc cgcacgtcga agatgcctgg ctcggtctgg
     1801 tccacgcttg ccgtggcgtc ctccataatg acacgccacg tctcgccgga gcctgtgatc
     1861 gggtccatcg ggatctcccg caggtagcct tcggtgatga ggtcctggag tgtctgcggt
     1921 gccttctgct tgtcgagcgt gtattcgtcg atcaccgtgc gcatggtgaa gagattgttt
     1981 ttcagcacgc tctccttggc acgtaggatt gaacgctggt agaggggaac ggcaatcgag
     2041 acgaggatcg tcacaatcgt catgacgatc atcagttcca cgagcgtgaa gccgcgcctc
     2101 ggattaccag tcggaatacg gcgtcccatc ggctgccttc tccgtgctct tggtgtagat
     2161 gtcaaagacg ttctgtccgc cccagctctt ggagtccggc tcgtcctgca tgcttcgcat
     2221 gccccactcg taggagtctg tcagcgggtc cttgggtatc cggcggagaa accggacctt
     2281 cttgtccacc gtgcccgaga gcttcactcc ctccaccaac acctggaggt tttccggata
     2341 gccctctgcg tccaccttcg gcggaccgag cagcccctga tcagagtagt ctttgtaacg
     2401 gtcgatcgcg ctgcggacct cgcgcagggc gtagcggagc tctttctctc gttcacgccg
     2461 cactttgtaa cgcgccaccg gaacggccat ggccgtcagc aggctcatga tggtgaaggc
     2521 gacaatcagc tccaccaacg tcagtccgct ctggttcttc cgtctcattc gtgttctact
     2581 tcacggtgac agtcacctgt ggcgatgcgg tcatgagcgg ctgcatctgc gagttctgag
     2641 gcgtgaactc gggcgccgtg acggtcgccg tgccgcgtcc cacaacctgg aaagtcagcg
     2701 tcagcagtgt tcccgatccg ctgactcccc ccgagccggg caggcggctc aagtttactg
     2761 tcgcatcgcc ggtatcgttc agaatgttcc gcgtaaagat gacctcctta ccgtcgccgg
     2821 caaggagatt cccgcgaacg atctcattca gccggagaat ggccgggtcg aacttcagac
     2881 gcagcggcgc cgagaacagg tccgtcacgt tttccacctg gagcgtgagg gtgaccgtcg
     2941 aaccgggttt cgcctccacc gcggcgggac tgaaaaggac cagtgccggc gtgccaggcg
     3001 ccggcggacc cgcgggcgct tcgggcttgg ccgcggccgg ttcgggcttc gtcgcttccg
     3061 gtttcggcgc ctcgggcttc ggaacaccgg gaaccggcgc ttccggcgct ggtgcgggct
     3121 tcggagcctc caccgcgggc gccgcctcgg gccggggcgc aaagctcagt ttgacgatgc
     3181 gctcgcttcc ggtggctatg cccttgaggt tctccttccg gagatcggcc gtccgcacca
     3241 cgtgagggat cagcgcgatg agcaactcgc tttcgttctt ctcgatgctc tcgcttgtga
     3301 acaggcggcg tatcacagga atgctggaca gtcctggaac gccggaaacc gcctttgtct
     3361 cctgcacctg catgagaccg ccgagcaggc tgacttcgcc ctccttcagc cggatgtcat
     3421 ggacaacctt ccgctggccg acgacgggct gcgagacgcc gccgatgtcg atccggtctc
     3481 gcacgttcga aatgtcgagc tcgatgtgaa gggagacctc atcaaggccg tgcaccttgg
     3541 gtgtgaggtc cacgttgact ccgacgtcca gaaactggaa agaggaatac aacgagttga
     3601 aacccgtccc cacctggccg aaggccggct ggaatccgcc ggtcgaatac ggcacgcggt
     3661 cgccgatctt cagagaggct ttctggccgt ccactgcccg gacctgcggc gactggagca
     3721 ctttcgtgcc gcggtcgctc atcaccgctt cgagaagtgc gccgggcatc gtgagcgagt
     3781 agtcgtttgt cgagatctta ccgatctgcg cgagcgagat cgcagtcgac tgaggggtcg
     3841 tgccagtgcc ggtgccggtg ccagtgccgg tgccagtgcc ggtgccggta ccagtgccag
     3901 tgccagtgcc ggtgccagtg ccagttccgg tgccggtacc agtgccacca gacagccggg
     3961 gattcctcgg ggtaaaggaa atcggcacgt tgattccggc ggcacctccg gaggcgatcg
     4021 tggccgagag gtcgcgcgac cgcgtgcggc ccacctccat cacgatgacg tcaaccacca
     4081 cctccggccg cggccggtcc agattgtgga tgatcagttc ggccagcgcc acctggtcgg
     4141 cggtgccgcg cacgataatc gcgctctgag agttgaagat gaacacgcgc tgggcgttgg
     4201 cgacggtgcg gatcgtgctc gagatctcat tcagctcctg cgaggtggtg acgttcgtaa
     4261 ggtagaacac cttggctacg gtgtcttcat actcggcacg cttctgctgg ttgtctgccg
     4321 tgacgaagac tgcgttggag gagagcggct tccagtacga cctcgtcatc acgccaacat
     4381 agtcgagcgc ctcctcgagc gtggcgttga ccacttccag gttcagcgct ttctgctgca
     4441 cctgtgtcgt gaagtcgggg tcgaacagga cgttgatacc cgccagcttg ccgaccgttt
     4501 cgaagagcac cttcggcggc tggttgttca tcttcagcgt gatcggagtg cgtgacagcg
     4561 gcttcagctc cggaacggat tgtacggtgg ccagcctctc ctcgatctcg cgttgggcct
     4621 cttctagagt tcccgcacct tgttcgcccc ccttctcgcc gggttgcgcc ttccgtttcc
     4681 gctcgcgctc gaccagatcg agcgtccgcc gcagctcctg cccggcgatc gcggaggagg
     4741 aatcgatggc gtgggcgcgc tcgaactccg ccagcgcctc ttcgagctgg ccctcggtcc
     4801 gcagtttctg tccctgattc acatgcattt gggcggcctg gaacctcatc cgcttggtcg
     4861 ccatctggta agctacgtcg gccgggtcct ccgccagtgc ctgctcataa aagcccaggg
     4921 ccttgtcgaa atcgttcctt acttcggcat cccggccctg ggcgaggaac cggtcgcctt
     4981 tcttgttgcc ggccagtccg gtcaaggcgc ccacgaggag aagcccgacc gctgccagaa
     5041 ggaggacaaa acggttctga atgcgcatct tattggtttt ccaaggcctg tgctaagata
     5101 aaggcgtcgc gatcgcctct cgtaatcaca taactgactg acgcagtatg gtttgcctcc
     5161 gtgcaggcaa aatatcgggc cgtggaagac cgcatcaaaa tcctaagtga caaccggcac
     5221 gccggacacc tctaccacct gttcgagcgc ttcgaagccg gcctcgctct gaccggaacg
     5281 gaggtcaagt ctgcccgtgc gggtaaggtc cagctcaagg acagttacgg agaagtgctg
     5341 ggcgacgaag cgtggctggt gaatgcccac atcagccact actctcacgg gaatcgggag
     5401 aaccacgacc ccgtccgccg ccgaaagctg ctccttcacc gcgacgagat taacaaactc
     5461 cacagcaaga cacgggagaa ggggctgacc ctcatcccga cgaaagtgta tctcaaaaag
     5521 gggcgcataa agtgcgaaat tgcgctcgca aagggcaaga aactgcacga taagcgcgaa
     5581 acggagcgca aacggacggc agaggccgag gcccgggccg ccctccgcca gcgctccctg
     5641 taacgacatc cctccggcct atggaaatcc aacttctatc ccaaccgatt gaaaacattg
     5701 aagcaaatgc cttagtcgtg gtcgctttcg acaaggagag ccccggcgaa gggctcggcc
     5761 ccgccgcgaa acgggccgac gagatggccg gcggctggat ccgagaggct tattccaccg
     5821 gcgagttctc cggcaagtta ctggaaacaa gcctcttcca tcgcccgcag gggctcaagg
     5881 ccagccgcct gctgctcgtc ggcggcggca aggcagaaaa gtttaccact gcggagcttc
     5941 gaaagctcgc cggagccgtg gttcgcatac ttaagtctaa gggatccaaa gacatagcgt
     6001 tcgccttgga cggttcgacg gcaaccgctt cgcacgtcgc cgcgacggtc gagggcgccc
     6061 ttctcggcga ctttgaaccc gatcgctata agactgaaaa aaaagaccaa aagaccatcg
     6121 cgcgcttctc cctggcggtg ccggcggtta ccgaagaact cgaggagtcc cttcgccggg
     6181 gccggattat cgcggagtca cagaatttca cccgtgacct cgtcagtgag ccggctaacc
     6241 gtttgaaccc cagcatctta gcggaacggg cacggctcat ggcagccgat tacggtctcg
     6301 agtacgaggt tctcgatgcc gaccggatgc ggcaggtcgg catgggtgcg ttgctcggag
     6361 tggcccaggg gagcagcgaa ccacccgcgc taatcatcct tcggtacagg ccggaaggcg
     6421 cggaccatgc ggcggcgcac ctcggcctcg tgggtaaggg cgtgacgttc gacacgggcg
     6481 gcatatcgat caagccttcc gagggcatgg agaagatgaa gtacgacatg gcgggcggcg
     6541 cggctgtgct cggggcgatg cgggcgctcg cgcagttgaa gccggcgatt ccggtgacgg
     6601 cgctcgtgcc tgccgtcgag aatatgcccg gaggaagagc gcaaaggccg ggagacatcg
     6661 tcaccacact ctccggcaag accgtggagg tcttgaacac ggacgccgaa ggacgcttga
     6721 tactggccga cgcgatcacg tatgcacgaa gactcggctg cacgcatctg gtggatgccg
     6781 cgacactaac gggggcgatc gtcgtggcgc tcggtcacgt gaacgtggga gcgttttcca
     6841 ccgacgatgc ctttcttggc aaggtcctcg acgcggcgaa gaatggcggc gagaagctgt
     6901 ggcagatgcc cctcgacgac gactacaagg aatccctgaa gagtgctttc gccgacattc
     6961 cgaacatcgg aaaccgttgg ggcggggcga tcacggcggc gatgttcctg aaggagttcg
     7021 ccgaggaaac accatgggta cacctcgata tcgccggcac agcctggctg gaagaggcca
     7081 agcccttcct cgcaaaaggg ccgtcgggcg tgccactgcg aacgctggtc aatcttgcgt
     7141 tgagttggcg gggctgaagt tccgaccgag ggccagggac cgcggccacg gggttgccct
     7201 gccaccccgc ctcgggattg tcggagagtc ggaacgaccg ttcccgtcca cctcgaccgg
     7261 tgtgacaccc ctcaggtttc tgacgccgta gatgccgagt tcataacggt ccctaacccc
     7321 cgccttctgg aacaggcggg agaggtagac cttgacagtt ccttcggtga ttgagagggc
     7381 ggaggcgatc tccctgttct tcatcccgaa agcgaggagt tcgagaagct ggctttcccg
     7441 gttcgtgagg cgcaccgttt caaccggcgg ccagccggcg gcgagcgtgc cgtccaggca
     7501 aacctcaccc gctgccacat cgcgcagccg gcctgacatt tgggccgcgt ccaacgtttt
     7561 tcgcagaatg gctcgcactc ccagttcgat cgcctggtag gcaagttcgg caggaatact
     7621 tcgggcccac agcacaatct tgcacgcagg agcagtccgc cggatgtcgg ccaccagcgc
     7681 gagtgcgacg tgatcgttgc agtcgatcac cagcacatcg ggctgcgccc ggagcacagt
     7741 tgcaggcaac gacgcggggt cgttccaaat ggaaaccagc tcgaatccac ccgccggcag
     7801 aaggagcgac atgaaaccac acgcactcac cggctcgtcc gtatataggg caatccgcac
     7861 catattcact ccttacggac tgtcgggctg taaacaggta attctttccg gcaactcgaa
     7921 aaaattaccg aggctacgat agattccctt ttatttcagt cgattccgac agtctcggga
     7981 aaggacggca gttttgttgc cgaacggatc ttgcgtatcg atcaccggga gtgaccgaat
     8041 tcggacttca ggaattcgag gacgaagcgg tcgcggagtt tcaagaccgc cttgatgtcc
     8101 ttcttcgacc agtcatagaa gcccctgccg gtcttgacgc ccaggtcacg gcgggcaaca
     8161 agttccttca tcaggggcgg cgcctgcggc tggttataga gatccttggc agtgtagtcc
     8221 atgatgccgt ggaccatgtc cagccccacg gcgtcggcgt gctcgaagat gccatacacc
     8281 ggcaggcgaa tgccgaagcc cagtttggcg gcgagatcga cgtcggcggc gtccgcgatg
     8341 ccttcttgaa cgatattcac cgcttcgcgc cacagcgcca tctggagcct gttgcccaac
     8401 tggcccgggc ggtcctttcg aaccatcacc ggcactttgc cgcaggcggc gagcagatcg
     8461 cgcaccgcgc gggcgacctc atcgggtgtt tgctcaccct tgacgatttc gaccagcggc
     8521 atgaggtgcg gcgggttcca gaaatgggtg gtcagcactc gttccggatg gcggcatttc
     8581 gaagcgacgg cggtgatgct caggctggag gtattcgacg ccaggacaga cgacggcggc
     8641 acgagggact ccatgcgttt gaacagttcc tgcttgaact ccatgttctc gggacccgac
     8701 tcgacaacga ggtcggctcc ggcgaccgtc tcgtcgaagt ttgccgtagc gctcagtgtg
     8761 tcgcgaagtt gttcgacgcg atccgaggtc agaagcgcgt tctcctcgag caagcggacc
     8821 tgctcactcg ctttttcgag tccggccacc gcgccgtcct gcgtacggga tacgatggcg
     8881 acgcgcaggc cgccgagagc caatgtcacg gcgatgccgg gccccatcat cccggtgccg
     8941 atcaccgcag cggattcgaa ccgtatctca cccatggaac cttccccgcc gggcgcaccc
     9001 ggcgtttaag cgcgtcttga aaactggttg gcaggtcggg agacctaccc tactaacgcg
     9061 gcgcgtactt ctgccgcacc agaaagacat aggatgcgac gccagagagc aaaaagatcc
     9121 agatggcctg catcggcgct tcatagctcc cggtagtctg tttcagccat ccggtcagca
     9181 gcggcgcgac gattcccggc aagttggcgg cgcagttctg gatgccgacg atccggccga
     9241 ccgcggcccc gggaatcaac gtctgcgtca gcgcccagta gttagccgtc gtcagtccga
     9301 ggccgctcaa cgagaacaca gcgaagaaga gcgcaacgga gtgcgagtcg gaaagggccc
     9361 cgaaaatctc ggtggaggcc accaggaagc ctgcgatggt gaacgccttt ctcaccctga
     9421 ccgggtcgcc cccgcggtcg atcatccggt cggccgccca gccggcggcg gtcgccacca
     9481 ccgccatgcc catgaaactg aacatcgtgt accaacccat cgactcgagc gagaggttgc
     9541 ggcgctcgac gaagtacgcg ggcatccagg tcatgcagaa gtagacgaag tattgataac
     9601 agaacgtccc gatgatcgtg ccccaaatga ccggactcgc cagaacccgg aggaagggta
     9661 cagacgcggc ggcattttgc acggcggcgc gctcgatctt ccggtcgtcg tcctcgacga
     9721 gcagcatcca tggcagaagc cagacaagac agccgagccc gaggatcacg aacatcgggc
     9781 gccaaccgta ggcgacgagt aaccatgtgg caagcggcgc tccgagcgcc gggccggcct
     9841 tggccgccgc catgtacaac ccgaccgcca gaccgcgctg gttctcggcg cagtggaagc
     9901 ggatccatct catgccggca ggagtgacga tcgattcgaa gacgcccagg agcatccgca
     9961 gcgcgaagag ctgccagagg gagcctgcca gcgctgccag cgccgacatg acactccaca
    10021 gaagaaaccc ggccgcgtag ggaaact
//
LOCUS       DATIFQ010000212        11248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1220166, whole genome shotgun sequence.
ACCESSION   DATIFQ010000212 DATIFQ010000000
VERSION     DATIFQ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220166"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..2293)
                     /locus_tag="VLE22_10030"
     CDS             complement(164..2293)
                     /locus_tag="VLE22_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HSB14788.1"
                     /translation="MADEKPTTREKTKRLGAWFLGPKAENASFEEEMILHVLRDYFYW
                     RRNYFPSDEIIVTQQMRREFLDWTDALLQQLSEMLARLRRHFPFYSPRYIAHMLSDQT
                     IPSVLGYFAGMLYNPNNVTPEAAPVTVEWELEVAADILKMLGYRCPPPPGAHHRELRR
                     EFGWAHITGGGTIANLEALWVTRNIRYFPPAVKRAAESHGIPIGIKLPGDSSHSPRSL
                     TEIDERQLLFIKPNEAVYLLPRFIDAVRQHLKLEEHAAAARAWEMLRSSGLLPSNAGL
                     VSTFANTPPALFVSGTAHYSLLKAADVLGIGKDNVVLVDVDRSFRMDVRDLEAKLRQA
                     IQRGLCPLAVIAIAGTTEEGAVDPIHHIDELRHKLEAEENLSFWLHADAAWSGYVRSV
                     FVPASPESESGDTEETIRRVNEFASRLLGLERGSYKKTIHLQWGSREVCNSFLAFPRA
                     ESITIDPHKMGYVPYPCGVVAFRNDRVRLYLTQEAPYITVTTSADLLSQAYEPPQNVG
                     PFILEGSKPGAAAAGCWLSHRMIPPNRDGYGEIMRASLLGARELYERLVHWEPGCRAN
                     WLETRYEFLPITGPPPDTNIVCFVVKIKGDRSLARMNRLGEKVYGHFTIEAELGEREY
                     AYAQPFFLSRTRFQTPQYSSHSVADLLERAGVDPEEYSAHGIFVLRATVMSPYIVFAA
                     ETGHKQDLLADFIETLAKTTEAVLAEV"
     gene            complement(2309..3007)
                     /locus_tag="VLE22_10035"
     CDS             complement(2309..3007)
                     /locus_tag="VLE22_10035"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSB14789.1"
                     /translation="MLEITWLGHGTFQFRLPSGEVILVDPWIEGNPTYPQRHEITRLD
                     TMLITHGHFDHVHDAVPLAKKFSPKVVAIFETCHWLESKGVTNTSPMNKGGSQQAGSV
                     TVTMTHAVHSCGILDGGQIIYGGEACGFVLTLPDSRRVYFAGDTNVFTDMQLIAQLYN
                     PELAFLPIGDLFTMGAREAAAACRLLRPHKVVPMHFATFPPLTGRPEQLAELLRDIPE
                     TQVWPLEIGKPVTW"
     gene            complement(3061..3522)
                     /locus_tag="VLE22_10040"
     CDS             complement(3061..3522)
                     /locus_tag="VLE22_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB14790.1"
                     /translation="MTSRSRSDASLHARILLVDDNKSGLSARRAILDELGHRTTTASG
                     GEDAIEQFSHGKFDILVTDYRMPKVDGLELIRRVRAVEPAIPIILLSGYVEAYGLDES
                     NTGADIVLPKSANEVAHLIRAVNRLLRRKVTRKPPGRQQTQPTSKRHTAGR"
     gene            complement(3638..4756)
                     /locus_tag="VLE22_10045"
     CDS             complement(3638..4756)
                     /locus_tag="VLE22_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB14791.1"
                     /translation="MKRIALATAALAGVSFALLIAQEGPKSQTSETVAKPRKKDAEAE
                     QPKIPSVFTKKDKPDLPEGTPTFRSDVLTVNLEVAVLSNKGQFIPGIPQGNFRILEDN
                     TPQQIATFEKGEAPMTVCLVIEFSNLFQYYWGETWYQTLTAAYGFLETLKPEDYVAVV
                     AYDMRPEILSDFSTDKRKAYEAMQRLRIAGFSEANLFDALTDTVERMSQIEGRKAVIL
                     ISSGMDTFSKLTFDKTRRILQNAGVPIYAIGLMQALREWYDARGAMGSIARLDFLQAD
                     NQLRTFTKETGGQAFFPRFYGEFPGIFRTISETLRNQYTITYQPTNQTRDGKFRKIKV
                     ELVNPATNEALRITDEKGKPLKYQIIAKNGYTAPREVE"
     gene            complement(4831..5586)
                     /locus_tag="VLE22_10050"
     CDS             complement(4831..5586)
                     /locus_tag="VLE22_10050"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSB14792.1"
                     /translation="MPSTARYSTSRSFYGRGVPTGVKWLLIVNSALFVLYFFAVRVGA
                     GSWFSDFSLWPRAVITLPAFWQLFTYMFLHDPYGFAHILFNMLALWMFGSDLEWDWGT
                     KRFLQYYFLCGVGAGVSVVVANVLFGSLDSRTIGASGAIYGLLLAFGVLYPDRIVLFS
                     FLFPIKAKYFVMIIGAIAFLSSIGASGGGVSHVAHLGGMVFGFLFLRRRKRQFNLTIP
                     VGRWYRQWKIHRARRKFDVYMRDRDSKRGPWVN"
     gene            5640..6284
                     /locus_tag="VLE22_10055"
     CDS             5640..6284
                     /locus_tag="VLE22_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14793.1"
                     /translation="MLRRRPSLVHPPGILERLYGRIRGREQMLVRGDPEWPLLLLSYP
                     RGALDEAKEVEAAYTHTFPLLTQETRSPYEALLPALPAIVVVLLRRRNACGCLGHYHP
                     RGTESRLTHRLASDLGSATGEIDIAYEAIREWQPQPLSSMALGDLGGRLAALHVRAAT
                     VAVLMHELEHLAFPDAPEREARGRSNALYSAVMSEFVAAETDSGYGMASSPPRP"
     gene            complement(6251..6541)
                     /locus_tag="VLE22_10060"
     CDS             complement(6251..6541)
                     /locus_tag="VLE22_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14794.1"
                     /translation="MLVRIRVRYRPARKTTISEERRRLLRSVENCLMSGSAAALLLVA
                     WCLATDLDWAGRFSIPSGRFSHWQLWLALAVLLQVLRSWLGRYGRGGDEAMP"
     gene            6625..7770
                     /locus_tag="VLE22_10065"
     CDS             6625..7770
                     /locus_tag="VLE22_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14795.1"
                     /translation="MRKLKAAVIGAGFMGRVHTESIRRLGNVEVAAVAAISAEEAAAF
                     GKAMGIERTSGDYRTLLADPKIDAVHVCTPNALHYPVSKAAMKAGKHVVCEKPLTLDV
                     TEAAEMVKLAKATKLANCVNHNLRYYPVVQQIRAMIAAGELGDILLVQGTYSQDWLLY
                     DTDYNWRIESKDNGRLRAMGDIGSHWMDMIQHLTGMKITALCADLQTFHKTRKKPKVA
                     IQTFAGKTLRPEEYDEVPIDTEDYGAVLLRMGDRARGAFTVSQVASGCKNRFQIEIFG
                     TKGGVMWNQERPDELWIGQRNSPNQLIMKDPSLLYPAAASYADLPGGHSEGYDDTHKQ
                     LHRRFYRKVADPSAPVEFPTFEDGLWGMKLLVKVLESVEKQGWVKTM"
     gene            complement(8007..8681)
                     /locus_tag="VLE22_10070"
     CDS             complement(8007..8681)
                     /locus_tag="VLE22_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSB14796.1"
                     /translation="MDFLIFDLDGTLIDSKLDLAQAVNATRVNLGLQPLENDRVYSYV
                     GNGAPVLIRKALGPDYGEDEVQEALEYFLAFYREHMLDYTVLYPGMRDCLDRLRDEGV
                     LMAVLTNKPVRFSTLILEGLGVADYFFRIYGGNSFEQKKPHPMGVEKLLAESSVPKEQ
                     TLMVGDSSVDIRTARNAGIRVCGVTYGFQPETLSEEPPDILLDRPEQLAELVISQRSS
                     KGGVRK"
     gene            complement(8740..9306)
                     /locus_tag="VLE22_10075"
     CDS             complement(8740..9306)
                     /locus_tag="VLE22_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HSB14797.1"
                     /translation="MNWLDIVLVAVLALSVLGGVAAGFAKVGIGFAAAVAGFFCGLWF
                     YGAAGSFFLPYVSHKGIAGFLGFALIFLAFAIAGVIIGKLLGMLFKWTGLSWLDRILG
                     AAFGLVRGLVIAVAIVVALMAFAPTRPPRSVVNSRFAPYVAGVAQMCALLAPREVKDG
                     VFESYERLRKVWSETVDKGLRKPDEKTF"
     gene            complement(9303..10193)
                     /locus_tag="VLE22_10080"
     CDS             complement(9303..10193)
                     /locus_tag="VLE22_10080"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HSB14798.1"
                     /translation="MTDNIILETDISDAELVARGKVRDVYRLDDKFLIVASDRISAFD
                     YILATGIPDKGRVLTQLSVFWFDFLKDVTPTHFLTANVDDYPDPLKRNRGQLEGRSML
                     VKRAKMIDIECVARGYLSGSGWKEYKQSGTVCGIPLPAGLKESDRLPEPIFTPATKAQ
                     TGHDENIPFERVVELIGSDLAGKLRDLTLSIYSKAARYAETRGIIIADTKFEFGFVGD
                     TLVLADEVLTPDSSRFWPKETYKPGGAQPSYDKQYVRDYLESIHWNKQPPAPALPPEV
                     AAGTSKKYKDAYRALTGKTL"
     gene            complement(10195..10662)
                     /locus_tag="VLE22_10085"
     CDS             complement(10195..10662)
                     /locus_tag="VLE22_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSB14799.1"
                     /translation="MLRDRSLVPLSQQHHNGLALCVLTERSLRADASPANVARLAKRA
                     IDRYEIELVNHFAIEEEILFPEIERRLGEHSLVRELIGEHRSIERIMGGLRGQPSAAL
                     LEELSSLLWTHIRREENELFEEIQKRLPREVLDALGEQIDAKAVRVCLESDGT"
     gene            complement(10723..>11248)
                     /locus_tag="VLE22_10090"
     CDS             complement(10723..>11248)
                     /locus_tag="VLE22_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14800.1"
                     /translation="SEERIRNWFFYRELSGDILVEQDCHNIDVINWFTGSHPVRVSGY
                     GSRQIRQYGDIFDNVAVTFQFADGLVFSYSANQFRTPGFQDVSETFICEKGAVNVSRQ
                     GYTIWRASGQPERAVTKYDITMDAVNEFIEGARTGKIENAAFSAAESTLTAVMALEAC
                     VLGRPMTWEEISRA"
BASE COUNT         2251 a         3377 c         3480 g         2140 t
ORIGIN      
        1 gcgcgagatt cccactttcc cacagctacg acgactacga ttccctccct cactgcaaga
       61 agaacttcaa agagggctgc ctaatgaaac cggacagatc acgtgtgaac aaaaccggac
      121 atcttgacct gttaacgaca gccggaacgc tgcaaacctc gcttcagacc tcggcgagca
      181 ctgcctcggt tgtctttgcc agcgtctcga taaagtcggc gagcaggtcc tgtttgtgac
      241 cggtttcggc ggcaaacacg atgtacgggc tcattacggt tgcgcgaagg acgaagattc
      301 cgtgcgcgga gtactcttcc gggtcgaccc cggcccgttc gagcaagtcg gcgacggaat
      361 gagaggaata ctggggcgtc tggaaacggg tgcgcgacag aaagaacggc tgggcatacg
      421 cgtactcacg ctctccaagc tctgcttcga tcgtaaagtg gccgtacacc ttttcaccga
      481 ggcggttcat tcgggcaagc gatcggtccc ctttgatctt gacgacgaag cagacgatgt
      541 ttgtgtcggg aggcgggccg gttatcggca gaaactcgta cctcgtctcc aaccagttgg
      601 cgcgacaacc aggttcccag tgcacgagcc gctcgtacaa ctcgcgcgcg ccgagtagcg
      661 aagcgcgcat gatctcgccg tacccgtcgc ggttcggagg gatcatgcgg tgcgagagcc
      721 agcagccggc ggccgcggcg ccgggctttg aaccctcgag aataaacggc ccgacattct
      781 gcggcggttc gtaagcctgg ctgagaagat cggcgctggt ggtgacggtg atgtagggcg
      841 cctcctgagt caggtacagg cggacgcggt cgttccggaa ggccaccacg ccgcagggat
      901 acggcacgta ccccatctta tgagggtcga tggtaatgga ctccgcgcgg gggaacgcca
      961 gaaaagaatt gcatacttcc cggctgcccc actgaagatg aatcgtcttc ttatacgaac
     1021 cacgctcgag acccagcagt cgtgaagcaa actcgttcac gcgccggatg gtttcctccg
     1081 tgtctccgga ctcgctttct ggcgaggcag gcacgaaaac ggaccggaca taaccggacc
     1141 aggcggcgtc ggcgtgcagc cagaaactga gattctcctc cgcttcgagc ttgtggcgaa
     1201 gttcgtcgat atggtgaatc gggtcgacgg cgccctcctc tgtggtcccg gcgattgcga
     1261 tgacggcgag cggacacagg ccgcgctgga tagcctgcct cagtttggcc tccagatcgc
     1321 ggacatccat ccggaagctg cggtcgacgt cgacgagaac gacgttgtcc ttcccgattc
     1381 cgagaacgtc ggccgccttc aggagactgt agtgcgcggt gcccgagacg aagagcgcgg
     1441 gtggcgtgtt ggcgaacgtg gaaaccaggc cggcattcga gggcagcagt cccgaggagc
     1501 ggagcatctc ccacgcgcgg gcggccgcgg cgtgctcctc gagcttgagg tgctggcgca
     1561 ccgcgtcgat gaaacggggc agcaggtaca ctgcctcgtt gggcttgatg aagagcaact
     1621 gtcgctcgtc gatttcggtg agcgaccgtg gcgagtggga agagtcgccc ggcagcttga
     1681 tgccaatcgg aatcccgtgg ctctcagcgg cccgcttcac cgccggcgga aagtagcgga
     1741 tgttgcgcgt cacccacagt gcttccagat tggcgatggt tccgccgccc gtaatgtgag
     1801 cccatccgaa ctcccggcgc agttcccgat gatgagcgcc cggtggggga gggcaacggt
     1861 aacccagcat cttgaggatg tccgccgcca cttcgagttc ccactccacc gtcaccggcg
     1921 ccgcctccgg agtgacattg ttcgggttgt acagcatgcc ggcgaagtag cccaacaccg
     1981 acgggattgt ctggtcggac agcatgtggg cgatatagcg gggactgtaa aacgggaaat
     2041 gccggcggag gcgcgccagc atctcgctga gttgctggag cagcgcgtcg gtccaatcca
     2101 ggaactcgcg ccgcatctgc tgcgtgacga tgatctcgtc ggacgggaag taatttcggc
     2161 gccagtagaa atagtcgcga agcacgtgga gaatcatctc ctcctcaaag ctcgcgttct
     2221 ctgcttttgg cccgaggaac caggcgccca gacgcttggt cttctcgcgc gtcgtcggct
     2281 tctcgtcggc catggcccgg tccgctcgct accaggtcac cggcttgccg atctcgagcg
     2341 gccaaacctg ggtttcgggg atgtcccgga ggagctccgc caattgttcc ggccgacccg
     2401 tcagcggtgg aaacgtggcg aaatgcatgg gcaccacctt gtggggacgc aacagccggc
     2461 aggccgctgc ggcttccctg gctcccatgg tgaacagatc gccaatcggg agaaaggcga
     2521 gctcgggatt gtaaagctgc gcaatcaact gcatgtcggt gaacacgttg gtgtcgccgg
     2581 cgaagtagac gcgccgcgag tcgggaagcg taagcacgaa accgcacgcc tctccgccat
     2641 agatgatctg gccgccgtcg agaatgccgc agctatggac ggcgtgtgtc atcgtcacgg
     2701 tgaccgagcc ggcctgctgg gagccgccct tgttcatcgg ggaagtgttg gtgaccccct
     2761 tggactcgag ccagtggcag gtctcaaaaa tcgcgacaac tttaggtgag aattttttcg
     2821 ccagcggtac cgcgtcatga acatgatcga agtgcccgtg cgtgatgagc atggtatcca
     2881 gtcgcgtgat ttcatgccgc tgtggatagg tcgggtttcc ctcgatccac gggtcaacca
     2941 gaatgacctc gccggaaggg agccggaact ggaatgtgcc gtgtccaagc cacgtgatct
     3001 ccaacatgag tgcgctcctc cactgataat aaaccgctgc ggaggcttgc cggtggccgc
     3061 tcagcggccg gcggtgtggc gcttggaggt gggctgggtc tgttgccttc ctggcggttt
     3121 gcgtgtgacc ttgcgtcgaa gcaaacggtt caccgcccgg atcaagtgcg ccacttcatt
     3181 cgcgctcttg ggaagcacaa tatctgctcc ggtgttcgat tcgtccaggc cgtaggcctc
     3241 gacgtagccg gagagcaaaa tgatcggaat cgccggctca acggcgcgga cgcggcggat
     3301 caactcgagg ccatccacct tcggcatacg gtagtcggtg accaggatgt cgaacttgcc
     3361 gtggctgaac tgctcgatag cgtcctcccc cccagaggcg gtggtggtgc ggtgcccaag
     3421 ttcatccagg atggctctcc gggcggagag gccgctcttg ttatcgtcga ccaggaggat
     3481 tcgagcgtga agcgaggcgt cggaacggga acgagatgtc attcgaacga gccgaaatta
     3541 acaaaggcgc aacaggactg tcaatcgttc tgtatcgaat tgtagcgaag cgagatgttt
     3601 cgcttgactt tttgggtgcg cgaagtgtga ggtccgttta ttcgacttcc cgcggcgccg
     3661 tgtacccgtt cttggcgatg atctgatact tgagcggctt gcccttctcg tctgtgatac
     3721 ggagcgcctc gttggtggct ggattcacaa gttccacctt gatcttgcgg aacttgccgt
     3781 cgcgcgtctg gttggtgggc tggtaggtga tggtgtactg attccggagt gtctccgaga
     3841 tggtccggaa gatcccgggg aattcaccat agaagcgcgg gaaaaaagct tgtccgccag
     3901 tttccttggt gaaggttcgg agctggttgt cagcctggag gaagtcgagc cgggcaatcg
     3961 atcccatggc tcctcgagcg tcgtaccact cgcgaagcgc ctgcatcagt ccgatggcgt
     4021 aaatcggcac gccggcgttc tgtaaaatcc ggcgggtctt gtcgaaggtc agcttgctga
     4081 acgtgtccat gccggacgag atcaggatta ccgccttgcg gccctcgatt tgcgacattc
     4141 gctccacggt atccgtgagg gcgtcaaaga gattggcttc tgaaaaaccg gcgattcgaa
     4201 gccgttgcat cgcctcgtac gccttccgct tgtcggtgga gaagtccgat agaatctcgg
     4261 gacgcatgtc gtaggcgacc acggccacgt aatcctcggg tttgagcgtc tcgaggaaac
     4321 cgtaagccgc cgttagcgtc tggtaccacg tttcgcccca gtagtactga aacaggttgc
     4381 tgaattcgat gacgaggcag accgtcatcg gcgcctcgcc cttctcgaac gtggcgatct
     4441 gttgaggcgt gttgtcttcg aggatccgga aattaccctg gggaattcct ggaatgaact
     4501 ggcccttgtt gctcaggacg gccacctcga ggttcacggt cagcacgtcg cttcggaatg
     4561 tgggcgtgcc ctccggcaga tcgggcttgt ccttctttgt aaacaccgac gggatcttgg
     4621 gctgttccgc ctcggcgtcc ttctttcgag gcttcgcaac cgtctcggac gtctggctct
     4681 tcggaccttc ctgcgcgatc aagagcgcaa acgacacccc cgcaagggcc gccgtcgcca
     4741 aggcgattcg tttcatggtg aaatcccccg ctcctactat cgactatagc aggggtccgg
     4801 agggtcggga cgcagccggc ggacgcccga tcagttcacc cacggaccgc gcttcgagtc
     4861 gcggtcacgc atgtaaacgt cgaacttcct gcgggcgcga tgtattttcc actgccggta
     4921 ccatcgcccc accgggattg tgaggttgaa ctgccgcttt cttcttctca ggaacaggaa
     4981 tccgaacacc atcccgccca aatgggcgac gtggctgaca ccgcccccgc tcgcgccgat
     5041 cgagctcaga aaggcgatgg cgccgatgat catcacgaag tacttggcct tgatcgggaa
     5101 caggaagctg aacaggacga tacgatcagg gtagagcacg ccgaaggcga gcagtagccc
     5161 gtagatcgct cccgaggcgc cgatggtccg gctgtccagg cttccaaaga gcacgttagc
     5221 taccaccacc gacacgcccg cgcctacgcc gcaaaggaag tagtactgga ggaagcgctt
     5281 ggttccccag tcccattcga ggtccgagcc gaacatccac agcgctagca tgttgaacag
     5341 gatgtgcgcg aacccatacg ggtcgtggag aaacatgtag gtgaatagct gccagaacgc
     5401 cggaagggtg atcacggcgc ggggccacag gctgaagtcg ctgaaccagg aacctgcccc
     5461 gactcggacc gcgaaaaagt acaaaacaaa cagcgcggag ttgacgatca gcagccattt
     5521 cacgcccgtg ggcacgcctc gcccatagaa agagcgggag gtgctgtatc tggcggtgct
     5581 tggcatgaag cagacggacc taccatgcaa gaataacaaa agcggtacac gcgatgaaaa
     5641 tgctgcgccg gcgcccaagt ctggtgcatc ctccagggat tttggaacgg ctatacggcc
     5701 gcatccgggg ccgcgagcaa atgctagtgc gcggagatcc ggaatggccg ctcctgctcc
     5761 tcagctaccc gcgtggcgcc ctcgacgagg cgaaagaagt cgaagcagca tacacccaca
     5821 cattcccgtt gctcacccag gaaacccgct cgccgtatga agcgctcctg ccggcgcttc
     5881 cagcgatcgt tgtggtgctg ctgcgccgcc ggaacgcgtg cgggtgcctg ggccactatc
     5941 acccgcgagg tacggagtcg cggctgacgc ataggttggc atccgatctc ggcagtgcga
     6001 ccggtgagat cgacatcgcc tacgaagcta tccgggagtg gcagccgcag ccgctttctt
     6061 caatggcgct cggagatttg ggcggccgcc ttgccgccct gcatgttcga gcggcgacgg
     6121 tcgcggtgct gatgcacgaa ctcgagcacc tggcgtttcc ggacgcaccg gagcgcgagg
     6181 cgcgcgggcg gagcaatgca ctgtactcgg cggtgatgtc ggaatttgtc gccgcggaga
     6241 ccgattccgg ctacggcatg gcctcgtctc cgccccggcc gtagcgccca agccacgatc
     6301 gtaacacctg aagcagcaca gcgagagcga gccagagttg ccaatgtgag aagcggcccg
     6361 aagggatgga gaaccggccg gcccaatcaa gatccgtcgc cagacaccac gcgacaagca
     6421 gcagcgcggc ggcggagccg gacatcagac agttttccac cgaccttagc agtcggcggc
     6481 gttcttcgga gatggtcgtt ttccgcgccg gacgataccg aacgcggatg cgaacgagca
     6541 tcgcagcact cctcgtttct tctcaattgt attagagtgt gatgtgacga agacaaatcg
     6601 ctacatccgg aaaggtggac tctcatgcgg aaactgaaag cagcagtgat tggagccggg
     6661 tttatgggtc gggtgcacac cgaaagcatc cgccggctag gcaacgtgga agtggccgcg
     6721 gtggccgcaa tttcggcgga agaggccgcg gcgttcggga aggcgatggg catcgagagg
     6781 acgagcggtg actaccggac cctgctcgcc gatccgaaaa ttgacgccgt ccacgtgtgt
     6841 acgccgaatg cgctgcacta tcccgtgtca aaagcggcga tgaaggccgg caagcacgtc
     6901 gtgtgcgaga agccgctgac gcttgatgtt acggaagccg cggagatggt gaaacttgcc
     6961 aaggcgacca agctggccaa ttgcgtcaat cacaacctcc gctattaccc ggtcgttcag
     7021 cagattcgcg cgatgatcgc cgctggcgaa ttgggcgaca tccttctggt acaggggacg
     7081 tattcccagg actggctgct gtatgatacc gactacaact ggcggatcga gtcgaaagac
     7141 aacggcagac tgcgtgcaat gggcgacata gggtcgcact ggatggacat gatccagcac
     7201 ctcaccggaa tgaagatcac cgcgttgtgc gcggacctcc agacgttcca taagacccgc
     7261 aagaagccca aggtcgccat ccagacgttt gccggcaaaa cgctgcggcc ggaggaatac
     7321 gacgaagtcc cgatcgacac ggaggactac ggtgcggttc tgttgcgcat gggcgacagg
     7381 gcgagaggcg ctttcaccgt gagccaggtg gcctcaggct gcaagaaccg gttccagatt
     7441 gaaatcttcg gcaccaaggg tggcgtcatg tggaaccagg aaaggcccga tgagctttgg
     7501 attggccagc ggaactcgcc gaaccagctc atcatgaagg acccttcgct cctgtatccg
     7561 gcggctgcaa gctatgccga cctgccgggt ggccacagcg aaggctacga cgacacgcac
     7621 aaacagctcc accgccgctt ctaccgcaag gtggctgatc cctcggcgcc ggtcgaattc
     7681 ccgaccttcg aagatggtct ctggggaatg aagctgctcg tgaaggtgct cgaaagcgtc
     7741 gagaaacagg gctgggtcaa aacgatgtag ctggcggccg ttgtggggcg gctccccaga
     7801 gccgctccag gccccccggc ccggtgctga attctaaccc tgcttcttct cggccgcgcc
     7861 gtccggacgc accagttccc accttccgcg gccgcaaagg ttcttgcagg acggtagggc
     7921 ctgcccttct gtgaagcgct ccgggcgctt gcacacgctg caccagtaaa tccctgtcga
     7981 gggtgccttg gtaccgggct tgtaggtcac tttcgaactc cacctttcga gctgcgctgc
     8041 gaaatcacga gttcggccag ttgctccggc cggtccagca ggatgtcggg cggctcttcg
     8101 ctgagtgtct cgggttggaa accgtaggtc acgccgcaga ctcggatgcc agcgttgcgg
     8161 gcggtccgga tatcgacgct gctgtcgccc accatcaacg tctgctcctt tgggacgctg
     8221 gattcggcca gcagtttctc gacgcccatc ggatgcggct tcttctgttc gaaactgttg
     8281 ccaccgtaaa tgcggaagaa atagtcggcg actcccagtc cttcgaggat gagcgtactg
     8341 aagcgcaccg gcttgttggt gagcaccgcc atcagaacgc cctcatccct caaacggtcg
     8401 aggcaatccc gcattccggg gtacagcacg gtgtagtcga gcatgtgctc acggtagaag
     8461 gcaagaaagt actcgagcgc ctcctggact tcatcttcgc cgtaatccgg accgagcgcc
     8521 ttgcggatca acaccggggc gccgttgccg acgtaggagt acacacggtc gttctccagc
     8581 ggttgcagac cgaggttgac gcgagttgcg ttgacggctt gcgccagatc cagtttcgag
     8641 tcgataagag taccgtcgag atcgaaaatc agaaagtcca tcagatcaag agcagacggg
     8701 cgagaccgcg ctaactccca tcctagcatc agcgaacggt cagaaggtct tctcgtcggg
     8761 tttccggagc cccttgtcca ccgtctcgga ccacactttc ctgaggcgct cgtagctctc
     8821 gaacacgccg tccttcacct cccgcggcgc aagcaaggcg cacatctgcg ccactccagc
     8881 tacgtatggc gcaaaacggg aattcacgac cgatcgcggc gggcgggtcg gcgcgaaagc
     8941 catcagagcg accacaatcg cgacggcgat cacgagtccc ctcactagtc cgaacgccgc
     9001 gccgagaatc cggtccagcc aggagagccc cgtccacttg aagagcatcc cgagcaactt
     9061 tccgatgatc acgccggcga tcgcgaatgc gaggaaaatc agcgcgaagc cgagaaagcc
     9121 ggcgatcccc ttgtggctga cgtaaggcag gaagaaggag ccagcggctc cgtaaaacca
     9181 cagcccgcag aagaagccgg cgaccgccgc cgcgaagccg attccgacct tcgcgaatcc
     9241 cgctgctact ccgcccaaca ccgaaagcgc gaggacggca accaggacaa tgtcaagcca
     9301 gttcacagag tctttccggt cagggcacgg tacgcgtctt tgtacttttt gctcgttccc
     9361 gccgcgacct ccggcggcag agccggcgca ggcggctgct tgttccaatg aatcgactcg
     9421 agatagtcgc gcacgtactg cttatcgtag gacggctgcg cgcctcccgg cttgtacgtc
     9481 tccttcggcc agaaccggga ggagtcgggt gtcaaaacct cgtcggccag cacgagcgtg
     9541 tcacccacga agccgaactc gaatttggtg tcggcgatga tgattcctcg agtctcggcg
     9601 tagcgggcgg ccttggaata aatcgagagc gtgaggtcac gaagtttccc cgcaagatct
     9661 gagccgatca gttccaccac cctctcgaat gggatgttct cgtcgtggcc ggtttgggcc
     9721 ttggttgcgg gagtgaagat cggctcgggc agccggtcgc tttccttcaa tccggccggc
     9781 aacgggatgc cgcacaccgt gccgctctgc ttgtactcct tccaacccga tccggagagg
     9841 tagccgcgag ccacgcactc gatgtcgatc atcttcgcgc gcttcacgag catggaccgg
     9901 ccctcgagct ggccgcggtt tcgcttcagc gggtccggat aatcgtccac gttcgccgtg
     9961 aggaaatgag tcggcgtgac gtctttgagg aagtcgaacc agaagaccga aagctgcgtc
    10021 aggactcgtc ccttgtcggg aatacccgtc gccagaatgt agtcaaaggc ggaaatccgg
    10081 tcgctggcga cgatcagaaa cttgtcgtcc agccggtata cgtcgcgcac tttgccgcgt
    10141 gcgaccaact ccgcatcgga aatatcggtt tcgagaatga tattgtctgt cacgtcaggt
    10201 tccatccgat tccaggcaaa ctcgaactgc ctttgcgtca atctgctcgc cgagcgcgtc
    10261 aaggacttca cgcggcaggc gtttctgaat ttcctcgaag agttcattct cctcgcgccg
    10321 gatgtgcgtc cacaagaggg aactcaattc ctcgagaagt gcggccgatg gctgcccacg
    10381 caggccgccc atgatccgct cgatgctcct gtgctctcca atcagctcgc gcactagcga
    10441 atgctcaccc agccgccgct cgatctccgg aaagaggatt tcctcctcga tcgcgaagtg
    10501 gttgacgagc tcgatttcgt accggtcgat ggcgcgcttg gcgagccgcg cgacgttggc
    10561 gggcgaagcg tccgctcgga gcgaccgctc ggtaagcacg cacagcgcaa gcccgttatg
    10621 gtgctgttga gacagcggta cgagggaccg atcgcgcagc atctacatac tggtagcaca
    10681 tcccgcgccg tcgcgtagcc gtggaatcag accgcgcagc cgttatgccc gggagatttc
    10741 ctcccaggtc atgggacgcc caagaacgca cgcttcgagc gccatcaccg cggtcaacgt
    10801 actctcggcg gcggaaaacg ccgcgttctc gatcttgccg gtgcgcgcac cctcgatgaa
    10861 ctcgttgacc gcgtccatcg tgatgtcgta tttggtcaca gcccgctccg gctggccact
    10921 ggcgcgccag atcgtgtagc cctgccgcga gacgttcacc gcgccttttt cgcaaatgaa
    10981 tgtctcggag acgtcctgaa agcccggagt gcgaaactgg ttggcgctgt aactgaacac
    11041 cagaccgtcg gcgaactgga aggtcactgc tacgttgtca aagatgtctc catactggcg
    11101 aatctgccgg ctgccgtagc cggagacgcg caccggatgc gatcccgtga accagttaat
    11161 gacgtctatg ttgtggcagt cctgctcgac gagaatgtcg cccgagagtt ctcggtagaa
    11221 gaaccagttc cgaatgcgct cttcgctg
//
LOCUS       DATIFQ010000213        12705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1220413, whole genome shotgun sequence.
ACCESSION   DATIFQ010000213 DATIFQ010000000
VERSION     DATIFQ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12705
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220413"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..519
                     /locus_tag="VLE22_10095"
     CDS             244..519
                     /locus_tag="VLE22_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14801.1"
                     /translation="MRRRIASPSEATVSFRRYGSGPPLILVYGSFSDDVTNWIFVKPI
                     LEKRFTVYAIARRDRGETDATEGHSLEDEARPCGDWDVTRPIETSGL"
     gene            complement(514..1236)
                     /locus_tag="VLE22_10100"
     CDS             complement(514..1236)
                     /locus_tag="VLE22_10100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB14802.1"
                     /translation="MADSLRGREIVLAGGSGGLGSATAELLAAEGARLVVSYRANEER
                     ASRLRDLAQIVRADLGSANDRKQLLDAAPALYGLVVFSGNPSRVTDLSGLEEVMRRSH
                     EVNYLGPILLAREAAARMKDKSTPGAIVLFSTMQAVSLFAGSTAYASPKLALLHAARI
                     LAKECRGRANIRVNVVCPGVTAAGMAEASIASGKYDRYLNEGMICRFGRAIDVARAVR
                     FFLEPDNYVTGQVLTVDGGLTL"
     gene            complement(1229..2470)
                     /locus_tag="VLE22_10105"
     CDS             complement(1229..2470)
                     /locus_tag="VLE22_10105"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSB14803.1"
                     /translation="MSPTQQTESFLGRAAFWLTGGAAVSVLFSIAACQILIALAFAAL
                     LLSGAKLRLPPIRLPLALFIAGTIVSLLLSDDPSAGRPQIRKFFVFLMLLVVYSTFRE
                     LAQIRRLALCWAGVGALVSARGLVQFAEKYGEARFHGQNFYEYYVGERITGFMSHWMT
                     FGGELMIVLLLLVAFVLFSPMARSKLLCFSIFCVTPISAALLLGFTRSIWIATGAAGL
                     YLLWSWRKIALLAVPVLFLAGIWFSPDSVKGRFVSMFKPRREVDSNQHRIVCWRTGWE
                     MIKAHPWFGLGPEQVKNQFNKYVPRDIPWPLPTGWYGHLHNIYIHYAAERGVPVMLVL
                     VWLLVKILFDFLRALRRIPPGPNDARFVLHGAVAVMISILLSGVFELNLGDSEVLTLF
                     LAVVAAGYVARDHVLQEASVG"
     gene            2506..3021
                     /locus_tag="VLE22_10110"
     CDS             2506..3021
                     /locus_tag="VLE22_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSB14804.1"
                     /translation="MVSETASDMLIHLLRHGIAEDASGTMKDADRALTADGRKKLLGV
                     LRAAKAAQVAPAVILTSSYLRAVQTAEVAAEVLGYEGELIRTKALEPGSSPEDVWEEL
                     RLYRQSSEVLVSGHEPLLSRTVAYLLGAPALQIDFKKGAMASILMEQFGAQPRGVLRW
                     LLTAKLVSRSS"
     gene            3059..4408
                     /locus_tag="VLE22_10115"
     CDS             3059..4408
                     /locus_tag="VLE22_10115"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HSB14805.1"
                     /translation="MKTIIEPFRIKSVEPLRHTTREERERFLERAGYNLFLIDARDIL
                     IDLLTDSGTSAMSTEQWAAMMRGDESYAGSESFHRMKAAVEDLTGFRHVIPTHQGRAA
                     ERILFSVMCRPGHIVPNNTHFDTTRANIEFTGARAVDLPLPEATDTQARLPFKGNMDV
                     VALGRLIETEGASRIPLVMLTVTDNSGGGQPVSMENIEAVRRVTSQHGIPFYLDACRF
                     AENCWFIGQREPGYGDWTPKRIAQKMFRLADGCTFSAKKDAFANIGGFLCTNDDRLAE
                     QETQLLILTEGFPTYGGLAGRDLDAIAVGLTEVLDPDYLRYRITSTAYLGRHIADRGV
                     PIVEPPGGHAIYIDAGRMLPHIPRDQFPAQALGVELYRHAGIRSVEIGSLMFGGKAHH
                     ELLRLAIPRRVYTQSHIDYVVEAILEVNQRKQDLRGLEIVSEPRFLRHFSCHLRPVR"
     gene            4685..5071
                     /locus_tag="VLE22_10120"
     CDS             4685..5071
                     /locus_tag="VLE22_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSB14806.1"
                     /translation="MDASVLIAVERGQLDLEAVSGPADADIAIAALTASELLHGVHRA
                     ATAAQRARREAFVEGLLARVPVIAFDLTATRVHARIWAETASSGVVLGAHDLVIAATA
                     IAAGARVATRDQRSFPKIPGLQWVAW"
     gene            complement(5109..5894)
                     /locus_tag="VLE22_10125"
     CDS             complement(5109..5894)
                     /locus_tag="VLE22_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14807.1"
                     /translation="MLKRSVCLVVFALLVAACLPGQEPKPLIVVASFKVHPAKADEWV
                     AGVKKIYVPALEKQFADGAILAYGLDEDFLHSAGKPNFEAWYVVQDYAALEKVDNALD
                     AAASEHKESMGQMMDAVDFDHHSDYVFRTVIGNHKMLPAGAKPYWHFSRVQVQPGKSE
                     EFRKGFEKYVKPTYDELVSSGAICGYDLDVEEVHSMEPGWHWTGICLPNLAAMDKVDA
                     AFDEVIKKRGPEEQAAVMTAYRELVVAGSHRDSVSVSRIFASK"
     gene            complement(5992..9225)
                     /gene="carB"
                     /locus_tag="VLE22_10130"
     CDS             complement(5992..9225)
                     /gene="carB"
                     /locus_tag="VLE22_10130"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSB14808.1"
                     /translation="MPRRNDIHRILIVGSGPIIIGQACEFDYSGTQACKALKADGFEV
                     VLVNSNPATIMTDPDLADRTYVEPLTRDFVEEVIRRERPDALLSTVGGQTGLNLSVEL
                     AETGVLEKYSVELIGAKLDAIKKAEDRLLFKDAMRKIGLETPQSQLVNKLEDGLEFAA
                     RIGYPVILRPSFTLGGTGGGIAYNREEFVEILERGLTLSPVHEVLIEESVLGWKEYEL
                     EVMRDLADNAIIVCSIENFDPMGVHTGDSITVAPAQTLTDREYQMMRDGALRCLREIG
                     VDTGGSNVQFAIDPETGRMVIIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYRLDE
                     IPNDITRKTPACFEPTIDYVVVKIPRWQFEKLPGAEPVLGTQMKSVGEVMSIGRTFKQ
                     ALAKGMRSLETGKAASAEVYEKELIRQKLITPNPDRLGYILFAFRSGYTIEQVRELTA
                     IDPWFLKQVKEAIELEDELQTESLETVSRERLAKAKRDGLSDSLLARMWKTDWLSIRN
                     RRKEMGIRAVFNRVDTCAAEFESFTPYLYSSYEQECEADPSDRRKVMILGSGPNRIGQ
                     GIEFDYCCCHASFALQKFGVESIMVNCNPETVSTDYDTSDRLYFEPLTLEEVLNIYDT
                     EKPHGVIVQFGGQTPLTLALPLKKQGIPIIGTDPSNIDLAEDRKLFGKLLDELGIPSP
                     ANGTATSVEEACEVARRIGFPVLVRPSYVLGGRAMVIAYDEETVRRYMREAVVFSRER
                     PVLVDRFLEDAVEVDVDALSDSEEVLIAGIMEHIEEAGVHSGDSSCVLPAISLGEAEV
                     ATIRDYTVRMARALQVIGLMNVQYAIKDGKVYVLEVNPRASRTVPYVSKATGVPLPKV
                     AVGLMLGKRLDELSHLTGRIVSGVLPVPQFFVKSPVFPFNKFPGVDPALGPEMRSTGE
                     VMGVGVNFGEAFGKAQLSAGTPLPDKGRVFLSVNDRHKAEAVEVGRKLSELGFELVAT
                     RGTAAALSAAGLHPKIIFKVNEGRPNAVDLIKAGSLQLVIYTATGAHSFSDERVIRRT
                     TVAYRVPCITTMSGAKAAAEALVSRGRDPIRVWSLQEIHSSEKSTVK"
     gene            complement(9232..10368)
                     /gene="carA"
                     /locus_tag="VLE22_10135"
     CDS             complement(9232..10368)
                     /gene="carA"
                     /locus_tag="VLE22_10135"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HSB14809.1"
                     /translation="MSRAILALEDGSVFEGTGFGALTERSGEVVFNTAITGYQEIFTD
                     PSYAGQIVILTYPEIGNYGTTPDDSESRKPYIEGLVVREFSSIASNWRSREHADAFLA
                     AHSIPVIAGIDTRSLVRRLRSRGVMRGVLSRAGSDPKELIAKALASPSMTGLDLATRV
                     TTPERYSWDEPVRSCSPSEDFGAPAEPRYNVAAYDFGIKRNILRRLVHVGCRVTVVPA
                     ETAAEEVLALKPDGVFLSNGPGDPEPLRFQAAQVRKLLGRTPVFGICLGHQVLGLAAG
                     GRTYKLKFGHRGANHPVINKIANRVEITSHNHGFAVDPDSLNPNEVEITHVNLNDQTL
                     EGFRLRNHPAFCVQYHPEAAPGPHDSLYLFDDFVNLMREYRTER"
     gene            10699..11784
                     /locus_tag="VLE22_10140"
     CDS             10699..11784
                     /locus_tag="VLE22_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14810.1"
                     /translation="MAVSVLLIGAASGQTHYVVRPVANPQFRQSQIFFRFEDIGSPPF
                     NELRRRFAFAETVTDIEDEFDRILRLRHWIFNTLKVDPSKPSPPMDAISILTQGPSGG
                     PFECSHAMIAQNAVMNAMGYVTRCLQPGPGQEENTYIAAGNHGVNEIWSNKHRKWFVS
                     DAEFDGHFEKGGVPLSALEIRDEYLKNKGKDVDLVVGPERRKVRPQLLFSPHCYRFVA
                     YELTGDRHTQSGWTSGDLVVWEDEYFKTHTWYRWSEPSGGKAAVHWAVRQNHFVRLAH
                     RDWIEWTPNVTSVKTTVQGEVAHVRLQSHTPNFDRYEIRRPGSRWEKTATDLNIKLEG
                     SRIEREFRSVNTAGVAGPVYRLLIEKE"
     gene            11855..12346
                     /locus_tag="VLE22_10145"
     CDS             11855..12346
                     /locus_tag="VLE22_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14811.1"
                     /translation="MSRKTWVGPVIAVLAVAGLSYSTLRYVAKRENHTCRACSRPVHE
                     HSRTVALINGNRGFYCCPACALSEHQQAGKPVEVTELTDYLGSGSLNPAESFIVRNSD
                     INMCARHEAALGPDKQPMHADFDRCSPGILAFRDLKAAQTFAAEHGGQVLRFTDLASE
                     YRR"
     gene            complement(12357..>12705)
                     /locus_tag="VLE22_10150"
     CDS             complement(12357..>12705)
                     /locus_tag="VLE22_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14812.1"
                     /translation="GKETDSAMKRAILGMVFASAIAAAAEKAQTFTGVITDTMCGTKH
                     TMGIKPDDKCVRECVKMDPKRWKYALLVGSEMYVLSDQQTPEKFAAQKVKVTGTLYEK
                     TKILKADKIEAAE"
BASE COUNT         2469 a         3945 c         3913 g         2378 t
ORIGIN      
        1 gcgcgagatt cccactttcc cacagctacg acgactacga ttccctccct cactgcaaga
       61 agaacttcaa agagggctgc ctaatgaaac cggacagatc acgtgtgaac aaaaccggac
      121 atcttgacct gttaacgaca gaccaagagc gggcggatca aggtccgatt gtgggccgtt
      181 tgagacaatt gtgagcgcag ggcaggataa acaaggccac cgtcctagtc gatgcgaggt
      241 tcgatgagga gacgcatcgc gtcaccaagt gaggcgaccg tctcgttccg gaggtacgga
      301 agcgggccgc cgctgatcct tgtgtacggg agtttcagcg acgacgtcac gaactggata
      361 ttcgttaaac cgattctgga gaaacggttc accgtctatg ccatcgcgcg ccgggaccga
      421 ggcgaaacgg acgcgaccga gggtcacagc ctggaggacg aggcccgtcc ttgcggtgat
      481 tgggacgtga ccaggcccat tgagacatcg ggactataac gtcaggccgc catcgacggt
      541 gaggacctgt ccggttacgt agttgtcggg ttcgaggaag aagcggacgg cgcgcgcgac
      601 gtcgatggca cggccgaagc ggcaaatcat cccttcgttc agataacggt cgtacttccc
      661 ggaggcgatg ctcgcctcgg ccattcccgc cgcggtcacg ccgggacaca cgacgttcac
      721 ccggatgttc gcgcgccccc ggcattcctt agccagaatg cgggccgcgt ggagaagggc
      781 caactttggc gacgcgtatg cggtggagcc agcaaacagc gagacggcct gcatggtcga
      841 gaagagcacg atcgcccccg gcgtgctctt gtccttcatg cgcgccgcgg cttcgcgggc
      901 gagcaggatc gggccgaggt agttgacctc gtgagagcgg cgcatcacct cttccaatcc
      961 cgagaggtcc gtcactcggg acggattgcc ggaaaagacg acgagcccgt agagagcagg
     1021 cgccgcatcg agaagctgtt tccggtcgtt cgcggaacca aggtctgccc gaacgatctg
     1081 agccaagtcc cgcaaccgcg atgcacgctc ctcgttcgcg cggtagctga cgaccagccg
     1141 tgcgccctcc gcggccagaa gttccgccgt cgctgagccg agcccgcccg aacctcccgc
     1201 cagaacaatc tcgcgcccgc gaagcgaatc agccaacgga cgcctcctgc aacacgtggt
     1261 cgcgcgccac gtatccggcg gccactacgg cgagaaacag ggtcaggacc tcgctgtcgc
     1321 cgaggttcag ttcgaagaca ccgctgagga gaatggaaat catgaccgcg actgcgccat
     1381 ggaggacaaa gcgggcgtcg ttcggaccag gcggaattcg tcgcagagcg cgcaaaaaat
     1441 cgaataggat cttgaccagc agccagacga gcaccagcat caccggcacg ccacgctcag
     1501 cggcgtagtg aatgtagatg ttgtgcaagt gcccgtacca gccggtgggc agcggccagg
     1561 gaatgtcccg cgggacgtac ttgttgaatt gattcttcac ttgttcgggg ccgagcccga
     1621 accagggatg ggccttgatc atctcccatc ctgtgcgcca gcagacgatt cggtgctgat
     1681 tggaatcgac ctcccggcga ggtttgaaca tcgagacaaa ccggcctttc accgaatccg
     1741 gcgaaaacca gataccggcc agaaacagca ccggcacggc cagcagcgcg atcttgcgcc
     1801 aggaccagag caggtacagc ccggcggcgc cggtggcgat ccagatgctc cgtgtaaatc
     1861 ccaaaaggag ggcggcactg atcggcgtca cgcagaaaat ggagaagcag agcagcttgc
     1921 tcctggccat cggagaaaac aggacgaaag caaccagcaa cagcagcacg atcatgagct
     1981 cgccgccgaa cgtcatccag tggctcatga agccggtaat gcgctcgccc acatagtatt
     2041 cgtagaagtt ctggccgtgg aatcgcgctt ctccgtactt ttcggcaaac tgcacgagcc
     2101 ctcgggcgga aactaacgcc cccacacccg cccagcagag cgcgagccgc cggatctgcg
     2161 cgagttcgcg aaaggtcgag taaacgacga gcagcatcag aaagacaaag aacttccgga
     2221 tctggggccg gcccgccgaa ggatcgtccg agagaaggag ggaaacgatg gtcccggcga
     2281 tgaagagcgc gagcggcaaa cgtataggtg gtagccggag cttcgcgccc gagagcagca
     2341 gcgcggcgaa ggcgagtgca atcaggatct ggcaggcggc aatcgaaaag agcaccgaga
     2401 cggcagcgcc gccggtgagc caaaaagccg cccgtccaag gaaggactca gtctgttgcg
     2461 tgggggacat gcgcctcccg ggaggaaaca cctcagtatg ccaaaatggt ctcggaaacg
     2521 gccagcgaca tgttgatcca tcttctcagg cacggaatcg ccgaggacgc cagtggaacc
     2581 atgaaggatg cggaccgggc gctgaccgcc gacgggagga agaagctact cggcgtgctg
     2641 cgcgcggcga aagccgcgca agtcgctccg gcggtgatcc tgacgagttc gtacctgcgg
     2701 gcggtgcaaa cggcggaggt tgccgccgag gtgctcggct acgagggcga gctgatccgg
     2761 accaaggcct tggagcccgg ctcttctccc gaggatgttt gggaggagct ccgcctctac
     2821 cggcagtctt cggaggtgct tgtgtccggc cacgagccgc ttctgagccg caccgtcgcc
     2881 tatctgctgg gagcgccggc gctccagatc gatttcaaga agggcgcgat ggcatcgatc
     2941 ctgatggagc agttcggcgc gcagccgcgt ggtgtgctgc ggtggcttct caccgccaag
     3001 ctcgtctccc ggagttcctg aaacgccgtc actcgcggcc ggtggtagac tccctcgtgt
     3061 gaagacgatc atcgagccgt tccgcatcaa gagcgtagag ccccttcgcc acaccacacg
     3121 ggaggagcgt gagcgttttc tggagcgggc cggctacaac ctcttcctga tcgacgcgcg
     3181 cgatatcctc attgacctcc tgaccgattc gggaaccagc gcaatgtcca ccgagcagtg
     3241 ggcggcgatg atgcgcggcg atgagtcgta cgccggcagc gagagtttcc atcggatgaa
     3301 ggcggcggtc gaggacctga ccggattccg gcacgtcatt ccgacacatc agggacgggc
     3361 ggcggaacga atcctcttca gcgtcatgtg ccggccgggg cacatcgttc cgaacaacac
     3421 acatttcgac acaacccggg ccaacatcga gttcactggc gcgcgggcgg tcgatctacc
     3481 gctccccgaa gcgacggaca cgcaggcgcg gctgcctttc aagggcaata tggatgtcgt
     3541 cgcgctaggg cggctcattg aaaccgaggg tgcgagccgg attcctctag tgatgctgac
     3601 ggtcaccgac aactccggcg gcgggcagcc ggtgtcgatg gagaacatcg aggcagtgcg
     3661 gcgggtcact tcacaacacg ggatcccgtt ttatctcgat gcctgccggt ttgccgagaa
     3721 ctgttggttc atcgggcagc gggaaccggg ctatggggac tggacgccga agcggatcgc
     3781 gcagaagatg ttccggcttg cggacggctg cacgttttcg gcaaagaagg acgccttcgc
     3841 aaacatcggc ggtttcctgt gtacgaacga tgaccggctg gccgagcagg agacacagtt
     3901 actgattctg accgagggct ttccgacata cggggggctc gcgggccgcg accttgacgc
     3961 gatcgctgtc ggtctgacgg aagtgctcga tccggattac ctgcgctatc ggatcacgtc
     4021 aaccgcctac ctggggcggc acatcgcgga tcggggcgtg ccgatcgtcg agccgccggg
     4081 gggccacgcg atctacattg acgccgggcg aatgcttccc cacatcccaa gagaccagtt
     4141 cccggcgcag gcgttgggtg tggaattgta ccggcacgcc ggtatccggt cggtcgagat
     4201 cggctcactg atgttcggcg gaaaggcgca ccacgaactg ctgcggctgg ccattccccg
     4261 ccgggtgtac acgcagagcc acatcgacta cgtggtcgaa gccatcctcg aggtgaacca
     4321 gcgcaagcag gatctccgtg gcttggagat cgtctccgag ccccggttcc tgcgccactt
     4381 cagttgccac ttgcggcctg tacgataatc tgataatcgt ggaatcggat atctctgcga
     4441 cgaacgccgc acgccgcttc ttggatctgc tcaaccgggt ggagtaccgt ggtgagacct
     4501 ttgtcgtctt gcgcggaaac acaccgatct gccggatcgc cccagtcggc ccgcgcgcat
     4561 gcacggtttc cgacctagca tcgtggctga aatccgcccc aaaaccggat gcctcctact
     4621 gggctgaaat cgaacaactt acgcggaatc agaccccgcc gccggacctg agatggtgac
     4681 gctgatggat gccagcgttc tcattgccgt ggaacgcggg cagttggatc tggaggcggt
     4741 ctccggaccg gcggacgcgg atatcgcaat cgccgccctg actgcgtccg aactgctcca
     4801 cggagtgcat cgagcggcga cagccgcaca gcgtgcccgg cgagaggcat tcgttgaggg
     4861 actgctcgca cgagtgcccg ttatcgcctt cgatctgacc gctaccagag ttcatgcgcg
     4921 aatctgggca gaaacggctt ccagcggagt tgtcctcggc gcgcacgacc tcgtgatagc
     4981 ggcgacggca atcgcggccg gcgcacgcgt cgccacccgc gatcagagaa gcttcccaaa
     5041 gatccccggc cttcaatggg tggcgtggtg acacgccgcc cgtgcgcgag tggactctct
     5101 gttgttgtct acttgctggc gaagatgcgc gacaccgaca cgctgtcgcg gtgcgatccg
     5161 gcgacgacca attcccggta cgccgtcatg actgccgctt gctcttcggg accgcgtttc
     5221 ttgatcactt cgtcgaatgc ggcgtcgacc ttgtccatcg ccgcaaggtt gggcaggcag
     5281 atgccggtcc agtgccagcc gggctccatg ctgtgcacct cttccacgtc caggtcgtaa
     5341 ccgcaaatcg ccccgctcga taccagctcg tcgtaagttg gcttcacgta tttttcgaag
     5401 cccttcctaa attcttcgct cttgccgggc tggacctgga ccctgctgaa gtgccagtaa
     5461 ggtttggccc cagccggcag catcttgtgg ttcccgatca cggttcgaaa cacgtaatcg
     5521 ctgtgatggt cgaaatcgac cgcgtccatc atctggccca tgctttcctt gtgctcgctc
     5581 gccgcggcgt caagcgcgtt gtcgaccttt tccaacgcgg cgtagtcctg tacgacgtac
     5641 cacgcttcga agttcggttt gcccgcacta tgtaggaagt cttcgtccag tccgtaagcg
     5701 agaatcgcgc cgtcggcgaa ttgcttctcc agcgccggca catagatctt cttgacgccg
     5761 gccacccatt catccgcttt ggcggggtga accttaaacg acgcgaccac aatcaacggc
     5821 ttcggctcct ggccgggaag acacgcagca acgagcagag cgaatacaac caggcagaca
     5881 gatctcttga gcatccagtt tacctccgag atttctttcg ggacagtatc ccagtgtctg
     5941 ccgattctaa agaaaggaaa ttactaaagt caatacgaaa tggtatgaat cttacttcac
     6001 cgtggatttc tcgctggagt ggatttcttg caggctccag acacggattg gatcgcggcc
     6061 acgcgaaacc agagcttcgg cagcggcttt cgcaccgctc atcgtggtga tgcagggcac
     6121 gcggtaagcg acggtcgtac ggcggatcac ccgctcatca ctgaacgagt gcgcacccgt
     6181 ggcggtatag atgactaact ggagcgatcc cgccttaata aggtccaccg cgttgggccg
     6241 tccctcattt accttaaaga tgatcttcgg atgcagcccg gcggccgaaa gagcggcggc
     6301 ggtgccgcgt gtcgccacca gttcaaaacc cagctccgag agtttccggc cgacctccac
     6361 cgcctccgct ttgtgacggt cgttcacact gaggaatacc ctgcctttgt cgggaagcgg
     6421 cgtcccggcg ctcagttgag ctttgccgaa ggcctcgccg aaattgacgc cgacgcccat
     6481 cacctcaccc gtcgaacgca tttcgggccc taacgccgga tccactcccg ggaacttgtt
     6541 gaacgggaag actggcgact tcacgaagaa ctgcggtacg ggcaggactc cggatacgat
     6601 cctgccagtc aagtgcgaca gttcatcgag ccgcttgccc agcatcaggc cgacggcgac
     6661 tttgggtagc ggcacgccgg tggccttact gacgtacgga acggtgcgtg acgcgcgcgg
     6721 attgacctcc agcacgtaga ccttcccgtc cttgatcgcg tactgcacgt tcatcaggcc
     6781 gatcacctga agcgcgcgcg ccatccgcac cgtgtaatca cggatggttg ccacctcggc
     6841 ctcaccaagg gagatcgccg gtagcacgca cgaactgtct cccgaatgga cgccggcctc
     6901 ctcaatgtgc tccatgatgc cggcaatcag cacctcttcg gagtccgaca gcgcatccac
     6961 gtccacctcg accgcgtcct ccagaaagcg gtcgaccagc acgggccgtt cacgcgagaa
     7021 aacgaccgct tcccgcatgt accggcgcac ggtttcctcg tcgtaagcga tcaccatggc
     7081 acgcccgccg agcacatagc tcggccgcac gagcaccggg aagccgatgc ggcgggccac
     7141 ttcgcaggcc tcctccacgc tggtcgcggt tccgttggcc ggcgacggga tgccaagttc
     7201 gtcgagcaac ttgccgaaga gcttgcggtc ctcggcgagg tcgatgttgg acgggtcggt
     7261 gccgatgatc gggatgccct gtttcttaag cgggagcgcc agtgtcagcg gggtctggcc
     7321 gccgaactgg acgatcaccc cgtgcggctt ctccgtgtcg tagatgttga ggacctcttc
     7381 cagggtcagg ggctcaaagt acaggcggtc gctggtatcg tagtcggtcg agaccgtctc
     7441 cgggttgcag ttgaccatga tcgactccac gccgaatttc tgcaacgcga acgacgcgtg
     7501 gcaacagcag tagtcgaact cgatcccctg gccgatacgg ttcggcccgc tcccgagaat
     7561 catgaccttg cgccggtccg acgggtcggc ctcgcactcc tgctcgtaac tggagtagag
     7621 gtacggcgtg aagctttcga actccgcagc gcaggtgtcc acccggttga ataccgcccg
     7681 gatacccatc tccttccgcc ggttgcggat ggaaagccag tcggtcttcc acatgcgagc
     7741 cagcagggag tcggagagcc cgtcgcgctt ggccttcgcc agccgctctc gggagaccgt
     7801 ttccagggac tccgtctgca actcgtcctc gagctcgatc gcttccttca cctgcttgag
     7861 gaaccatggg tcgatggcgg tcagctcgcg gacctgctcg atcgtgtacc cgctgcggaa
     7921 cgcaaagagg atgtaaccga gacggtctgg gttcggcgtg atcagcttct gccggatcag
     7981 ctccttttcg tagacctcgg ccgaggccgc tttgccggtt tcgagacttc gcatgccctt
     8041 ggcgagggcc tgcttgaacg tccggccgat cgacatcact tcgccgacgg acttcatctg
     8101 tgttcccagc accggttcgg cacccggcag tttttcgaac tgccatcgcg ggatcttgac
     8161 caccacgtaa tcgatggtgg gttcgaagca ggcgggcgtc ttgcgggtga tatcgttggg
     8221 gatctcgtcg aggcggtaac ccaccgctag cttggcggcg atcttggcaa tcggaaatcc
     8281 ggtggccttc gaagcgagcg ccgaggaccg cgacacgcga gggttcattt cgatgatcac
     8341 catgcgtccc gtctcgggat cgatggcaaa ctgcacgttc gagccgcccg tatcgacgcc
     8401 gatctcgcgc aggcaccgca aggcgccgtc gcgcatcatc tggtactcgc ggtcggtaag
     8461 cgtctgcgcg ggcgcgaccg tgatcgaatc gccggtgtgg acgcccatcg gatcgaagtt
     8521 ctcgattgag cagacgatga tggcgttgtc ggcgaggtcg cgcatcacct cgagctcgta
     8581 ctccttccag ccgagcacgc tttcctcgat cagcacctcg tgcacgggcg acagcgttaa
     8641 cccgcgctcc agaatttcga cgaactcctc gcggttgtag gcgatgccgc cgcctgtgcc
     8701 gccgagggtg aagcttggcc gcaggatgac cgggtagccg atgcgggcgg cgaactccag
     8761 gccgtcctcg agcttgttga cgagctgcga ttgcggcgtc tccagcccga tcttccgcat
     8821 cgcgtccttg aaaagcagac ggtcttcggc tttcttgatc gcgtcgagct tcgcgccgat
     8881 caattcgacg ctgtactttt cgagcacgcc cgtctcggcc agttcaaccg ataggttcag
     8941 cccggtctgc ccgccgacgg tcgagagcag cgcatccggc cgctcgcggc gaatcacctc
     9001 ctcgacgaag tcgcgcgtca atggctcgac ataggtacgg tcggcgaggt cgggatcggt
     9061 cataatggtg gccgggttcg agttgaccag caccacctcg aaaccatccg ccttcaaggc
     9121 tttacaggcc tgcgtgccgg agtagtcgaa ttcgcaggcc tggccgatga tgatcggtcc
     9181 ggaacccacg atcaggattc gatggatgtc gttgcgcctg ggcatccgtg cttaccgttc
     9241 cgtcctatat tcccgcatga ggttgacgaa gtcgtcgaag aggtaaaggg agtcgtgagg
     9301 ccctggagca gcctctggat gatactgcac gcagaacgcc gggtgattgc gaaggcggaa
     9361 tccctctagc gtctggtcgt tcaggttgac gtgcgtgatc tcgacctcgt tcgggttcag
     9421 cgagtcggga tcgacggcga aaccatggtt gtgagaagtg atttcgaccc ggttggcgat
     9481 cttgttgatg accgggtgat tcgcaccgcg gtgtccgaac ttcaacttgt acgtgcggcc
     9541 gccggcggcc agccccagca cctgatgccc gagacagatg ccgaaaaccg gcgtccgtcc
     9601 caacagcttg cgaacctggg ccgcctggaa ccggagcggc tcggggtcgc ctggaccgtt
     9661 cgacagaaag acgccgtcgg gcttgagcgc aaggacctct tcggcggcgg tttctgccgg
     9721 caccacggtc acgcggcagc cgacatggac cagccggcgg aggatgttcc gcttgatgcc
     9781 gaaatcgtac gcggccacgt tgtagcgcgg ttccgccggc gccccaaagt cctcggacgg
     9841 cgagcaggaa cggaccggct cgtcccatga gtaccgttcc ggcgtggtga cgcgcgtcgc
     9901 caggtccaga cccgtcatag acgggctggc gagcgccttc gcgatcagtt ccttcgggtc
     9961 gctgcccgcg cgggacaaaa ctccccgcat cacgcccctc gaacggagcc gccggacgag
    10021 gctgcgggtg tcgatcccgg cgatgacagg aatgctgtgc gcggccagaa acgcgtcggc
    10081 gtgctcccgg ctgcgccagt tgctggcgat cgacgagaac tcgcgaacca ccagcccctc
    10141 gatgtaaggc ttccgcgact cgctgtcatc cggcgttgtc ccgtagttgc cgatctcggg
    10201 gtaggtaaga atgacgatct gtccggcata cgagggatcg gtgaaaatct cctggtagcc
    10261 ggtgatcgcg gtattgaaga cgacctcgcc ggagcgctcg gtgagcgcac caaagcccgt
    10321 gccctcgaag acggagccgt cttccagggc gaggatcgct ctactcaatc ggatggtcct
    10381 cccgggaagg tgtaactcct atttgagcac ttcggagcgc cacggttcaa ccgtcccgat
    10441 ggtggcgcca acctcgcccc cttccgcgga tcgtccggaa gatgacccgg atcggcttgg
    10501 aggagccccg ggggtttgtt gaataacctg gttggcaggt ccggagacct gccccacgtc
    10561 gacggaagtc gttgctctgc aaggtagcgt aggactccgg tcctgcgtca gcgaggttgc
    10621 ccgccaggtt cttcaagaag ctcctagact aggagggtgg ttcccattgg ctcacaaacg
    10681 gcatcatcgt acgcacgagt ggcggtatca gttctgctga ttggcgccgc gtccggccaa
    10741 acacattacg tggtgcgccc ggtggcgaac ccccagttcc ggcagagcca gatattcttc
    10801 cggttcgagg acatcggttc acccccgttt aacgaactgc gccgccggtt cgccttcgcg
    10861 gagactgtaa cggacatcga ggacgagttc gaccgaatcc tgcgcttgcg gcactggatc
    10921 ttcaacacat tgaaggtcga cccctcaaag ccgagcccgc caatggacgc catctccatc
    10981 ctcacccaag gtccgtcggg tgggccattc gaatgctcgc acgccatgat cgcgcagaac
    11041 gccgtcatga acgcgatggg ttacgtaacc cgctgtctgc aaccaggtcc ggggcaggag
    11101 gaaaacacgt acatcgcagc cggcaatcac ggcgtgaacg agatctggtc gaacaagcat
    11161 cgtaagtggt tcgtctccga cgcggagttt gacgggcact tcgagaaggg gggtgtcccg
    11221 ctttcggctc ttgagattcg cgacgagtac ctgaagaaca aaggaaagga cgtagatctc
    11281 gtggtcggac cggagagacg aaaggtcagg ccccaactct tgttctcccc gcactgctat
    11341 cgctttgtcg cctatgaact gacgggcgac cgccacacgc aatcgggctg gacctccggg
    11401 gatctggttg tctgggagga cgagtacttc aagacgcaca catggtatcg gtggtcagag
    11461 ccgagtgggg gcaaagcggc cgtgcactgg gcggtgaggc aaaaccactt cgtccgactg
    11521 gcccatcgcg actggatcga gtggaccccc aacgtcacga gcgtgaagac taccgtacaa
    11581 ggcgaagtcg cgcatgtccg cctgcagtcc cataccccca atttcgaccg ttacgagatc
    11641 cgccgaccgg gcagccgttg ggaaaaaact gctactgacc tcaacatcaa gcttgaaggg
    11701 agccgcattg agcgtgagtt ccggtccgtc aacacggcgg gagtcgccgg acccgtttac
    11761 cgccttctga tcgagaaaga ataggccgtc cggttagcgc cccgcttgcc cggacttcac
    11821 tccggatgta acattggacc agagagaggc ggctatgagt cggaagactt gggttggtcc
    11881 cgtcattgct gtcctggccg tcgccggctt gagttactcg accttgcgat acgtcgcgaa
    11941 acgagaaaac cacacttgca gagcctgctc ccggcccgtt catgagcatt ctcggactgt
    12001 ggcgttgatc aacggcaacc ggggattcta ttgctgtcca gcctgtgcgc tgtccgaaca
    12061 tcagcaggcg ggcaagccgg tcgaagtcac cgaactgacc gactatctgg gaagcgggtc
    12121 tctcaacccc gccgaatcct tcatcgtccg caacagcgac atcaatatgt gcgcacgcca
    12181 cgaggcggcc ctaggcccgg acaagcagcc aatgcatgcc gacttcgatc gctgctctcc
    12241 aggcatactc gcctttcggg atttgaaggc agcacagacc ttcgcggccg agcacggggg
    12301 ccaagtactg cgattcaccg atttggcgtc cgagtaccgc cgctgaaagg ccgcggctac
    12361 tccgccgctt cgatcttgtc agccttcaag attttcgtct tctcatagag agttccggtt
    12421 accttcacct tctgtgcggc gaacttctcg ggcgtctgct ggtcgctcag cacgtacatt
    12481 tcgctgccga ccaatagtgc gtacttccac ctcttcgggt ccatcttcac gcactcgcgg
    12541 acgcacttgt cgtccggctt gatacccatg gtgtgtttgg tgccgcacat tgtgtcggtg
    12601 atgacgccag tgaatgtttg tgccttctct gccgccgcag cgatagccga ggcgaagacc
    12661 ataccaagaa ttgctctctt cattgcgctg tctgtttcct ttcct
//
LOCUS       DATIFQ010000214        16726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2561735, whole genome shotgun sequence.
ACCESSION   DATIFQ010000214 DATIFQ010000000
VERSION     DATIFQ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16726
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561735"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VLE22_10155"
     CDS             <1..625
                     /locus_tag="VLE22_10155"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSB14813.1"
                     /translation="GAIQTRSSRLLIPAAMKPDTYSIWLSAGEFRGTMEFMRAYVVYS
                     DDHGATWRRGALVRALTNENQLVELSDGTVLMDARQNAGGRRWMIASKDGGASWSEPH
                     PGETVTAVATAIERFTSAAAGDDHDRIIWTGPSGPGRQTLVVRVSYDEARTFPKERVL
                     YSGKAAYSDLDILKDKTAGLLWERDDYKFITFTRCNREFLEGGPQGR"
     gene            complement(629..1390)
                     /locus_tag="VLE22_10160"
     CDS             complement(629..1390)
                     /locus_tag="VLE22_10160"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSB14814.1"
                     /translation="MSEFQGKTALVTGASRGIGSATATALARQGVSRVLIHYNSYREG
                     AEKTLVALKQAGADGGLLQADLGTVDGIHSLLEQLRASGPKVDILINNAGSLVKRAKL
                     LEFTEELYDEVMNLNVKSAYLIAQAVAPHMIERKNGIIVNLSSIAGRNGGGPGATVYA
                     AAKAAVTCLTKGMARELAPLGIRVNAVSPGTVDNYFHEKFSTREILDQVVNMTPAGRL
                     STNEDVADVIVFLCSNAARYVHGQTIEINGGMLAP"
     gene            complement(1403..2197)
                     /locus_tag="VLE22_10165"
     CDS             complement(1403..2197)
                     /locus_tag="VLE22_10165"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSB14815.1"
                     /translation="MEHLLSLIRQARVYDLAQPYFTGMPHWPTHPPFQFSLSKLHGEI
                     RFGTGASSAAEAIALGGHVGTHMDALCHFTFDGKLHGGEAVAGVQSYGGGLERMSIDT
                     IPPILRRGVLLDIAGHRGVEALAEDFVVTPEHLESAAKTHGVDVRAGDVVVVRTGWAR
                     YWNEPGRYINGLREPGPEETGARWLSERSVFAAGSDTAAFERVPSPEMRVHVHLLVES
                     GIHILEALNLEELAAARVYEFLFVAAPLKIRGGTGSPIRPFAVTVS"
     gene            2189..3112
                     /locus_tag="VLE22_10170"
     CDS             2189..3112
                     /locus_tag="VLE22_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14816.1"
                     /translation="MFHKFELYYPRMKRAIRIMVSRHSAFYSPLIAAIAAGFLEQEGI
                     EATYGVLQPGQRSHALLREGAVDVFQSAVSSSWGPLEKGERDLPVHFAQVNQCDGFFL
                     VARRPIHDFEWTMLEAAAVLADHGGQPLAMLKYAARCQGVDWNRIRVIDAGSPPEMER
                     VFRGGSGDFVHLQGPVAQQLEVEAIGWIVASAGEAMPPVAFSSLLARREFLETDEAHA
                     FLRAYRRAREWADQAPAREVARAEAHFFPGVSFNALGAAVARYQQLGCWDGGIEIPRA
                     LYEQALEVFLTAGTIGRRHPYEEVVVTPCIE"
     gene            3100..4035
                     /locus_tag="VLE22_10175"
     CDS             3100..4035
                     /locus_tag="VLE22_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB14817.1"
                     /translation="MHRMITRRELLAAAAGTAAAQTARPKGLLIDTHIHLFAADQTRF
                     PYHRNAVYRPPAQPLEDYAKFVRAAGIDHTVIVHPEPYQDDHRYLEYCFENEPRSRFF
                     KGTCLFDPIDPATPERIEALVKKHPGRIVALRIHENRAPGQPPSASGAIRERDLQAPA
                     MRETWRKVHALGLAIQMHFVPHFAPQIRALAEVFPEMPVILDHLGRAGEGTPAEYEEV
                     LKLAKLPRTYMKYSAVRYSSKQEYPHRDVKPLIRRVYDAFGPDRIIWGGLGMNLEEFQ
                     RQARLLDEMFDFASEADRAKIRGLNAARLYGFGKT"
     gene            4056..5294
                     /locus_tag="VLE22_10180"
     CDS             4056..5294
                     /locus_tag="VLE22_10180"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HSB14818.1"
                     /translation="MIRRTLFVGALLCALCVPAAAEKYRRLPAKEYLDKMKAGWIGQI
                     IGVSWGAPTEGKFKTIMPAEKMPPFSEDLVNNAFGQDDLYVEMTFLRTLEMYGFDASI
                     RQAGIDFANSEYPLWVANAAGRSNLRKGIAPPDSSHPQFHKSAGAIDYQIEADYSGLI
                     APGMPGAVIALGEKFGRLMNYGDGVYAGQFMGCMYAEAFFETDILKIIDAGLRCIPLQ
                     SMYAGMVKDMLRWRRENPSDWEKTWALVDRKYRQDKNYYVSPLDVKLEGAFVLMGMLY
                     GKGDPDQTIIISTRCGSDSDCNPSSAAGVLFTSRGLSKLPGRYYRKLDETKIFSHTAY
                     NFPGLMAVCDKLARQALAKAGGRVEKNSKGEELFVIPVRPPQVSKFEDIKNPGPAAGS
                     LFTAEEKARITKQDPPRKSK"
     gene            5584..8445
                     /locus_tag="VLE22_10185"
     CDS             5584..8445
                     /locus_tag="VLE22_10185"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSB14819.1"
                     /translation="MQSSFRLIAVVAAFCCASFADSTLAPSGAILLNGRPFPMVLDAG
                     TDCFTPQDYDTVMASKDAWGANTWWLQYSMLHMRSEREGDFTGLARELDFFERTGMMV
                     NLYVRGEYRDVPEWFYKKYDDYRMQDPAGKPVGGQICFQHEGFRLLIDGYVRGVARAA
                     RDKKALLMYSTYDEFGPRGWGCFCPRCVRKYREYLKAKFGGVMALNGAWGTKYAGWED
                     VDAPRTQSFDANYGEWQRYRLSVLRDFGLLYYNALKQEDPRHLRWIDINMDLFDYTWR
                     WGVIWWKLTDIFDMLNNGPEGYDVNAPIRTAMSRAITDNFGKGGTWHLGFPRNEVLPR
                     GELYSRLFDHSHGGLVWWYSFWDVLRGGRAWGGGEEEGSPVVGNWFRARELNHLARYL
                     GDLYVHSKPVRGKVGLFVSGTTDLMRSLSAKQALQKGDPTELSGMGQILRDLNIAYEA
                     IGEDQLSKLGSFQTVVLGQFSMCGDEKTAATFREYVRGGGNLIVLNGAFSADVNGRET
                     ANPAFGLEDVWGSAGAVADQMPDGRIAAGGGIEVPSLGGVARRKMGGAHVIGSLPDGT
                     PAVTVNKFGKGRVLFFGTNAGEAYNAGYLLTRGQYFLPRGQKLDLAKYAELVRLYRGW
                     QGYAALVRDFLREAGVTSPVRAVAAGEPDLFGKVRVSVQEQRTLGRETVNHLLIVTLE
                     PVYDPEAQIKTGAAKYGPRSVKDLVLRVRLPAAEQVKAVYRVPPVGYEMGKMDAVPGR
                     VPFAAEGDEIRLTLAAPTEAECLLVARDARPLVGVKSDAVSAKDGQATPLTVTVDNAT
                     GWPISGEITFPASFRVVAAQGNAAKFASLKPGERYAARFEVTAPTPIDRHRTFAATVR
                     YRGAGGEGEAASYPVTSRTDERIAWSWLQKAEAGMTDVTITPGIPRGRPYAEALQKRE
                     FAYAAYNEGAWADAVRLARELVMTCNQLKAQRREPGPPR"
     gene            complement(8579..9646)
                     /gene="cax"
                     /locus_tag="VLE22_10190"
     CDS             complement(8579..9646)
                     /gene="cax"
                     /locus_tag="VLE22_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HSB14820.1"
                     /translation="MNWLLVSVPIAIGLRFLLPDAHILIFFAACLAILPLAGWLGKAT
                     EHLAERTGEGIGGLLNATFGNAAELIIALMALQKGLHEVVKASLTGSIIGNILLVLGA
                     AVIAGGLRYRSQQFNAVAARSQSTMLTLAAIGLIVPAGYHHLAGPGMEVREGSLSLEI
                     SIVLLATYGLSLLFSLHTHKQLFVGNAAESAGVQGGHKVWSAGRSISVLLGATALVAW
                     ISEILVGSVEHTAEALGMTSVFVGVFVVAIIGNAAEHSTAILVALKNRMDLSLGIAIG
                     SSIQIALFVAPVLVLASYVIGPRPLNLVFTPAEVAAVGLAVLIVGQIAVDGESNWLEG
                     VQLLAVYVVLGIAFYFLPEAR"
     gene            complement(9691..10167)
                     /locus_tag="VLE22_10195"
     CDS             complement(9691..10167)
                     /locus_tag="VLE22_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB14821.1"
                     /translation="MVKPIPDGCSTVTPYLVVKDAAKAIDFYKRAFGAQEVMRMPGPG
                     GHGVMHAEIKIGDSLIMVSDEFPGTNVTSPETAGTTTASLFVYFEDVDAAFQRAVGAG
                     GKPVMPPADMFWGDRYGKLVDPFGHHWGLATHKEDVIPEEMSKRADAFFASMAAGQ"
     gene            complement(10275..11273)
                     /locus_tag="VLE22_10200"
     CDS             complement(10275..11273)
                     /locus_tag="VLE22_10200"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HSB14822.1"
                     /translation="MKELAIIGAGSWGTALSLVLAPRFPGVRLWIYEQDLATRMVKLR
                     ENDLYLPGFQLPANVRVETELAAALGDAEIVLGVMPSHHARRLYTEMLPYLKPTMRIV
                     SATKGLEEGTRQRMSEVIRHVAGRRFEPMVAALSGPTFAREVARGEPAAVVIASEDPA
                     LAAEIQAAFSGPNLRLYTNTDLLGVEIAASLKNVIAIGAGVCHGLGLGNNTNAALITR
                     GLVEMTRLAVALGARPATLSGLAGLGDLVLTCTGELSRNRSVGIKLARGHRLEEIVAS
                     MRMVAEGVKTTYSAVELGRQLGVDLPITNQMYEVLRNGKPPREAIRELMERSLKPE"
     gene            complement(11270..11878)
                     /gene="plsY"
                     /locus_tag="VLE22_10205"
     CDS             complement(11270..11878)
                     /gene="plsY"
                     /locus_tag="VLE22_10205"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HSB14823.1"
                     /translation="MTIGILSLLIAYLLGGVPFGYLLVKWTSGRDVRTSGSGNIGATN
                     VLRTAGRAAGITTLVLDIAKGYVAVWLVGRMTGDSPLWTSAAALAVMLGHAFPAALKF
                     KGGKAVASCVGGFLRLTPLPLAAVVVVFAVTVAVTRYISLGSIIAAGSLPLAVWLISH
                     PEAPVTAAALLAGAFVIWRHKSNIQRLHAGNENVFSFGGRRT"
     gene            complement(11875..12522)
                     /gene="thpR"
                     /locus_tag="VLE22_10210"
     CDS             complement(11875..12522)
                     /gene="thpR"
                     /locus_tag="VLE22_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HSB14824.1"
                     /translation="MGFRLFFSLQVRGYSSENGYGKEIITAFPFPTMRLFTAIDISTA
                     VKSNLETLVGRLRPTAKLKWSPPGNLHITTKFIGEWPEERLEELKPALAALPQREAFE
                     IAVQGLGWFPNPHSPRVFWAAVRAPAALADLARETDAAMARIGVPREPKPFAPHLTLA
                     RIKEPVPLAAVRQAIAGLESDDFGRFTADRFYLYLSEMRPSGSVYTRLAEFPFER"
     gene            12498..13136
                     /locus_tag="VLE22_10215"
     CDS             12498..13136
                     /locus_tag="VLE22_10215"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HSB14825.1"
                     /translation="MRKRAEIPFRDIGTAAGILPRSFYSRPAAEVARDLLGKILVHGR
                     AAGRIVETEAYLGLGDRAAHASRGITPRTKVLFGPPGHAYVYFIYGMYECLNLVAEPE
                     GTPGCVLIRALEPLAGLAAMRRRRPHARTNDELASGPGKLTLAMGITRRHNGADVTSG
                     SLTVRSWPDDSRFEIGVSPRIGIRHCRDWPLRFFIAGNPCVSRHPVAGPSAS"
     gene            13149..14510
                     /locus_tag="VLE22_10220"
     CDS             13149..14510
                     /locus_tag="VLE22_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HSB14826.1"
                     /translation="MLKPTIFREYDIRGIADTELLDDGIEQLGRAIGSYLQRRSGKSI
                     NLGRDCRLSSTRLRNALVKGLVASGCDVTDIGTVPTPLLYYSVFHLKATGAVMITGSH
                     NPPEFNGFKVMAGPSTIYGAQIQEVREILEKMDFIEGQGSLRTEDVSGSYIDQVAANI
                     RLSRRVTVVFDAGNGTAGPIMRRLLDRLNCDPVELFFDMDGKFPNHHPDPTVEKNLAH
                     LKATVAAQKAELGIAFDGDADRIGAIDELGNVIWGDQLMLIYAREILTRKPGSTFIGE
                     VKCSQVMYDELKRLGGNPIMWRTGHSLIKAKMKEAHAELAGEMSGHIFFADRYYGFDD
                     ALYAACRLLEIVADSGKPLSHQLAGLPKMVSTPEIRVDCPDELKFGVVNRVTEHFKKV
                     RDVVDVDGVRVLFEQGWGLVRASNTQPVLVLRFEAANGALLAQYQREVETVLDEAKAA
                     SGV"
     gene            complement(14535..14825)
                     /locus_tag="VLE22_10225"
     CDS             complement(14535..14825)
                     /locus_tag="VLE22_10225"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HSB14827.1"
                     /translation="MATLLIQFSYLQVLDILTTLAFLLHGVQEGNPLIRMLMGAAGSP
                     LAGLVAAKALALMLAVYCWRLRRQRVLAWANGMYALLVVWNIVALMVRGVTV"
     gene            complement(14991..>16726)
                     /locus_tag="VLE22_10230"
     CDS             complement(14991..>16726)
                     /locus_tag="VLE22_10230"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HSB14828.1"
                     /translation="FAVSLILVWFLRSPPRSSEAVLVPVPLTSYPGEEGYPSFSPDGT
                     QVAFQRCPEGFWSGKNCDIYVKQIGVEPPSPLTDTPAQEFSPAWSPDGQTIAFLQGLS
                     PDASSFSLVLIPQRGGRERVLAELDFSGSTNALSGGPYLAWTPDSKWLACPLPEAGQL
                     ALYLFSVETGEKRKLTSPPAEPSTDTAPAFSPDGHTLAFARLFYVGPKSQLYLLRLGE
                     GYQPLGEPQRARLNHPDSFSPAWTPDGKEIVFASSAGLWRLAAASPGTPRRLPFAPDN
                     AFAPAVSRKGNRLAYLVGRFDTNIWRIDLRGANHKPGAPVPFISSTWWEAGPAYSPDG
                     KRIAFVSSRSGGREIWVCDADGSDAVQLTSLGGPAIQGPSWSPDGQKLAFSWEPGGNQ
                     DVYVVSAAGGMPRRVTADPGADKWPYWSRDGQWLYFTSDRGGSQEIWKMPAGGGEAVQ
                     VSRDSGADLPHESPDGKFVYYSKGWPFPQSVWRVPAEGGEGTKVLDAVHPLALWTIGK
                     EGIYFFTVPDKKRHSDLSIYEFATGKTRKILTIERPLGAFFAVSPDGRTILYTQVDEG
                     GSDLMLVENFR"
BASE COUNT         3143 a         5405 c         5188 g         2990 t
ORIGIN      
        1 cggtgcgatc cagacccgga gcagccgtct tctgattccg gcggccatga agccggacac
       61 atactcgatc tggctttccg ccggtgaatt ccggggcacc atggagttca tgcgcgcata
      121 cgtggtttac agcgacgacc acggtgcgac gtggcggcga ggtgcgctcg tccgcgcgct
      181 gaccaacgag aaccagctcg tggaactttc ggacgggacg gttctgatgg acgcgcggca
      241 aaacgcaggc ggccggcggt ggatgatcgc cagcaaggat ggtggcgctt cgtggtctga
      301 accgcatccc ggggagaccg tcacggcagt cgccaccgcc atcgaacggt tcacttcggc
      361 cgccgccgga gacgaccacg accgcatcat ctggacgggg ccgtccggtc ccggcaggca
      421 gacgctggta gtccgggtga gctacgacga agcccgaacc tttccgaaag agcgcgtcct
      481 ctattccggg aaggccgcgt actccgacct cgacatccta aaggacaaga cagccggact
      541 cctctgggag cgggatgact acaagttcat taccttcacc cgatgcaacc gggagttcct
      601 ggagggcggg ccacagggga gatgaggctt acggcgccag catcccgcca ttgatctcga
      661 tcgtctgtcc gtgaacgtag cgcgcggcat tggaacacag gaacacaatc acgtcggcta
      721 cgtcttcgtt cgtgctgaga cggcccgccg gcgtcatatt caccacctgg tccagaatct
      781 cccgcgttga gaatttctcg tggaagtagt tgtctaccgt ccccgggctt acggcattga
      841 cccggatgcc gagcggagcg agttcccgcg ccatcccctt tgtcaggcac gtcaccgccg
      901 cttttgcggc tgcgtagacg gtggccccgg ggccgccgcc attccgcccg gcgatcgagc
      961 tcaggtttac gatgatgccg tttttccgct cgatcatgtg cggagccacc gcctgtgcga
     1021 tgaggtaggc gctcttcacg ttgaggttca tcacctcgtc gtagagttcc tcggtgaact
     1081 cgagcagctt cgcccgcttc accagtgagc ccgcattgtt gatgaggatg tcgacttttg
     1141 gtcccgatgc ccgcagttgt tcgagcagcg aatggatacc gtccacggtg ccgaggtctg
     1201 cttgaagcag tccgccgtcg gcgcctgctt gtttcaacgc caccagtgtc ttctccgcac
     1261 cttcacgata gctgttgtaa tgaatcagga cgcgtgagac tccctgccgc gctagtgcgg
     1321 tcgccgtcgc cgatccaatc ccgcgggacg cgccggtgac gagtgctgtc ttgccttgga
     1381 attcgctcat agagaaacca gattacgaca ccgtaaccgc gaatggacga atcggcgaac
     1441 cggtcccccc gcggatctta agaggcgcgg ccacgaagag aaattcgtag acccgcgcgg
     1501 cggccagctc ctccaggttg agcgcttcca gaatgtggat gccgctctcc accagcaggt
     1561 gcacgtgtac gcgcatctcc ggcgacggca cacgctcaaa cgcggccgtg tccgagcctg
     1621 cggcgaagac gctccgctca ctgagccagc gggcccccgt ctcttccggt ccgggttcgc
     1681 ggaggccgtt gatataacgc ccaggctcat tccagtagcg cgcccatccc gtgcgcacaa
     1741 ccacgacgtc acccgcccgc acgtcgacgc cgtgcgtctt ggccgccgac tccagatgtt
     1801 ccggcgtcac gacgaagtct tccgccagcg cttccacgcc gcgatggccg gcaatgtcga
     1861 gcagcacgcc gcggcggagg atcggcggaa tggtgtcgat ggacatccgc tccagcccgc
     1921 ctccgtaaga ctgcacgccg gccaccgcct cgccgccgtg cagtttcccg tcgaacgtaa
     1981 agtggcagag cgcatccatg tgcgttccga catgtccgcc tagtgcgatc gcctccgcgg
     2041 cagagctcgc accagtcccg aatcggatct ccccgtgcaa cttggagagg ctgaactgga
     2101 acggcggatg cgtcggccag tgcggcatgc ctgtgaaata gggttgcgcc aggtcgtaga
     2161 cccgggcctg ccggatcagg ctcaggagat gttccataaa ttcgaactat actacccgag
     2221 gatgaagcgc gcgatccgta tcatggtctc ccgccactcg gcgttctaca gcccattgat
     2281 cgccgccatc gccgccggct ttctcgaaca ggaaggcatt gaggccacct atggggtgct
     2341 acagcccggt cagcgttcgc acgctctgtt gcgcgagggc gcagtggacg tgttccagtc
     2401 ggcggtttca tccagttggg gtccgctcga aaaaggcgag cgagatctgc cggttcactt
     2461 cgcgcaagtg aaccagtgcg atggattctt tctcgtcgcg cgccgtccga tccatgactt
     2521 cgagtggaca atgctcgaag ctgcggccgt gcttgcggat cacggcgggc agccgcttgc
     2581 gatgctgaaa tacgccgccc gatgccaggg cgtcgactgg aaccgaatcc gggtgattga
     2641 cgctggctcg ccgccggaaa tggaacgtgt ctttcgcggc ggatcaggcg atttcgtgca
     2701 ccttcagggg ccggtcgcgc agcaattgga ggtggaggcg atcgggtgga tcgtcgcatc
     2761 ggccggggag gcgatgcccc cggttgcgtt cagcagcctg ctcgccaggc gtgagtttct
     2821 cgaaacggac gaagcacacg ccttcctgcg cgcctaccgc cgcgcccggg agtgggccga
     2881 ccaggcgccg gcccgcgaag tggcccgcgc cgaggcgcat ttcttcccgg gagtgagttt
     2941 caatgcgctt ggcgccgccg tcgctcgtta ccagcaactc ggctgctggg acggcggcat
     3001 cgagattccg cgcgcgctct acgagcaggc cctggaggtc tttcttacgg ccggcacgat
     3061 cggccgccgt catccctatg aggaggtcgt cgttactcca tgcatcgaat gatcacccgt
     3121 cgcgaattgc ttgcggccgc cgccggcact gctgctgcac agacggcgcg gccgaaaggt
     3181 ctgctgattg acactcatat ccacctgttc gccgccgatc agacacgctt cccgtatcat
     3241 cggaacgcag tctatcgtcc gccggcgcaa ccgctcgagg actacgctaa gtttgttcgc
     3301 gcggccggaa tcgatcatac cgtcatagtg caccccgagc cgtaccagga cgaccaccgg
     3361 tatctggagt actgcttcga gaacgagccc cggtcgcgtt ttttcaaggg gacgtgcctg
     3421 ttcgacccga tcgaccccgc cacgccggaa cgaatcgagg cgctggtgaa gaagcatccc
     3481 ggccgcatcg tcgcactgcg catccacgaa aaccgtgccc ccggccagcc gccttccgcc
     3541 tccggggcca tccgcgagcg ggatctgcaa gcgcctgcca tgcgcgaaac gtggcgcaag
     3601 gtccacgctc ttggcctcgc catccagatg cacttcgtcc ctcattttgc gccgcagatc
     3661 cgcgcgctgg cggaagtgtt tcccgagatg cccgtgatcc ttgaccacct cggccgagcc
     3721 ggagaaggaa cgccggccga gtacgaagag gtgctgaaac tggcgaagct gccgcgaacg
     3781 tacatgaaat actcggccgt gcggtactcc tcgaagcagg agtacccaca ccgcgacgtg
     3841 aagcctctga ttcgccgcgt ctatgacgcc ttcggtccgg accgaattat ttggggcggc
     3901 ctgggaatga acctggagga attccagcgc caggcgaggc tgctcgacga gatgttcgac
     3961 ttcgcctcgg aagcggaccg cgcgaagatt cggggcctga acgccgcgcg cctgtacggc
     4021 ttcggcaaga cgtaacatac accctggaga cgcttatgat acgaagaact ctttttgttg
     4081 gtgcgctgct ctgcgccctg tgcgtaccgg cggccgcgga gaaataccgg cgcctgccgg
     4141 cgaaggaata tctggacaag atgaaggccg gctggatcgg ccagatcatc ggggtttcgt
     4201 ggggcgctcc caccgaaggc aagttcaaaa cgatcatgcc ggcggagaag atgccgcctt
     4261 tctcggaaga cctcgtcaac aacgcgttcg ggcaggacga cctctacgtc gaaatgacgt
     4321 ttctgcgaac cctcgagatg tacgggtttg acgcctcgat tcggcaggcc ggcatcgact
     4381 tcgccaacag cgaatacccg ctatgggtcg ccaacgctgc cggacggtcg aacttgcgca
     4441 agggcatcgc gccgcccgat tccagccacc cgcaattcca caagagcgcc ggtgctatcg
     4501 actatcagat tgaggccgat tattccggac tgatcgcgcc cggcatgccc ggtgcagtca
     4561 tcgcgctcgg cgagaagttc ggccgtctga tgaactatgg cgacggcgtg tacgccggcc
     4621 agttcatggg ctgcatgtac gccgaggcgt ttttcgaaac ggacatcttg aagatcatcg
     4681 acgccggctt gcgctgcatc ccgctgcagt cgatgtatgc cgggatggtg aaggacatgc
     4741 tccgatggcg gcgtgagaac ccgtccgatt gggagaagac ttgggcgctg gtggatcgga
     4801 agtaccgcca ggacaagaac tactacgtga gcccgctgga cgtgaaactc gagggcgcct
     4861 tcgtgctcat gggcatgcta tatggcaagg gcgaccccga ccagacgata atcatctcga
     4921 cccgctgcgg ttcggattcc gactgcaatc cgtcgagcgc cgccggcgtg ctctttacga
     4981 gccgcggtct ttcgaagttg cccggccggt actaccggaa gctcgatgag actaaaatct
     5041 tcagccacac ggcgtacaac tttcccggct tgatggcagt gtgtgacaaa ctcgcccgcc
     5101 aggcgctcgc gaaggcgggt ggccgtgtcg agaagaactc aaagggtgag gagctcttcg
     5161 tcattccggt tcggcctcct caggtgagca agttcgaaga catcaagaac cccggccctg
     5221 ccgccggcag tctgttcact gccgaggaga aggcgcggat cacgaagcaa gacccgccgc
     5281 gaaagtcgaa ataacggaag agtcggtggt gggtcagttt cctgatagaa ctctctcatc
     5341 caggttacgt acgccacgac agttcggcga ttcctcaagg ctgggcgtcc acacgcacat
     5401 gtgggggata cgtcgcccgt cctaggtttc acctcatccc ttcaaagcag cacggccgca
     5461 gacgaaatac ccgctgttcc agcgatcacc accgaaaacc atcgcaggcc tgcatcagct
     5521 tcggtttaga ctggcgcaac atcaacgctg atacaatttt cgagaacgtg aggacacacc
     5581 gtcatgcaat catcgttcag gctgatcgcc gtggtcgcgg cgttttgctg tgcttcgttt
     5641 gctgacagca cgctcgcccc ctctggggcc attcttttga acggtcgccc tttccccatg
     5701 gtgctggacg ctggcacgga ctgcttcacc ccgcaggact acgacaccgt gatggccagc
     5761 aaggatgcct ggggcgcaaa cacctggtgg ctgcaatatt ccatgctgca catgcgctcg
     5821 gagagggagg gcgacttcac cggactcgcg cgcgagctag atttcttcga gagaaccggc
     5881 atgatggtga acctgtatgt gcgcggcgag taccgtgacg taccggagtg gttctacaag
     5941 aagtacgacg actaccgcat gcaggatccc gcgggtaaac cggtgggcgg gcagatctgc
     6001 ttccaacatg agggcttccg gctcctgatc gacggctacg tgcgcggggt ggcacgcgcc
     6061 gcgcgcgaca aaaaggcgct gctgatgtac tcgacttacg acgagttcgg cccgcgcgga
     6121 tggggctgct tctgcccccg ctgcgttcgc aagtaccgcg agtatttgaa ggcgaagttc
     6181 ggcggcgtaa tggctctgaa cggtgcgtgg gggacaaaat acgcgggctg ggaagacgtg
     6241 gatgcgcctc gcacgcagtc cttcgacgcc aattacgggg agtggcagcg ctatcggctg
     6301 agcgtgctgc gcgatttcgg gctgctctac tacaacgccc tgaagcagga ggacccacgc
     6361 catctgcggt ggatcgacat caacatggac ctgtttgact acacctggcg ctggggcgtg
     6421 atctggtgga agctcaccga catcttcgac atgctgaaca acgggccgga ggggtacgac
     6481 gtgaacgcgc ctatccgcac ggctatgagt cgggccatta cggataattt cggcaagggc
     6541 ggtacgtggc acctaggctt cccgcgcaac gaggtgctgc cccgcgggga gctgtactcg
     6601 cggcttttcg accacagcca cggcgggctg gtgtggtggt attcgttctg ggacgtcctg
     6661 cgcggcgggc gtgcctgggg cggtggagag gaggaaggct cgccggtggt gggcaattgg
     6721 tttcgtgcgc gcgagttgaa ccatttggcg cggtacctgg gcgacctcta cgtgcactcg
     6781 aagccggtgc gcgggaaggt ggggctgttc gtcagcggga cgacggacct gatgcggagc
     6841 ctgagcgcca aacaggcatt gcagaaaggc gaccccacgg agctttcggg aatgggccag
     6901 attctgcgcg acctgaacat cgcgtacgag gccatcgggg aggaccagct ctcgaagctg
     6961 ggatcgttcc agacggtggt gctgggccag ttctcgatgt gcggggatga gaagaccgcg
     7021 gcgacattcc gggagtacgt gcgcgggggc gggaatctca tcgttctcaa cggcgcgttc
     7081 tcggcggatg tgaacgggcg agagaccgcc aatccggcat tcggcctcga agacgtgtgg
     7141 ggcagcgcgg gcgcggtggc agatcagatg ccggacggcc gcatcgcggc gggcggcggg
     7201 atcgaggtgc cgtcgctggg cggcgtggcg cgccggaaaa tgggcggcgc gcatgtaatc
     7261 ggcagcctgc cggacggcac gccggcagtg acggtaaaca agttcggcaa gggccgggta
     7321 ctgttcttcg gcaccaatgc cggggaggcg tacaacgctg ggtatttact cacgcgcggc
     7381 caatactttc tgccgcgcgg gcagaagctc gatttggcta agtacgcgga actggtgcgg
     7441 ttgtacaggg gttggcaggg ctacgccgcg ctggtccggg atttcctgcg cgaagcgggc
     7501 gtgaccagcc cggtgcgggc ggtggcggcc ggtgagcccg acctgttcgg caaggtacgg
     7561 gtgagcgtgc aggagcagag gacgctcggg cgcgagacgg taaatcacct gttaatagtc
     7621 acgctggagc cggtctacga cccggaggcg cagatcaaga ccggggcggc gaagtacggg
     7681 ccgcgcagcg tgaaagacct ggtgctgcgg gtgcggctgc cggcggcgga gcaggtgaag
     7741 gcggtgtacc gcgtcccgcc ggtgggttac gaaatgggca agatggacgc ggtgcccggg
     7801 agagtaccgt tcgccgccga aggcgacgag atccggttga ccctggcggc gccgaccgaa
     7861 gccgaatgcc tgctggtggc gcgcgacgcg cgtccgctag tgggtgtgaa gtcggacgcg
     7921 gtgagcgcga aggacggaca ggccacgccg ctgactgtca cggtagacaa cgccacgggt
     7981 tggccgattt cgggtgaaat cacgttcccg gctagcttcc gcgtggtggc ggcgcaagga
     8041 aacgcggcga agttcgcgtc gttaaagccc ggcgagcgtt acgcggcgcg ctttgaggtg
     8101 accgcgccca cacccatcga ccggcaccgg acctttgcag cgacggtgcg ttaccggggc
     8161 gcgggagggg agggcgaagc ggcctcctac cccgtcacga gccgcaccga cgagcggatc
     8221 gcctggagct ggctccagaa ggccgaagcc ggcatgacgg acgtaaccat tacgccgggg
     8281 attccgcgcg gacggccata cgccgaggcg ctgcaaaagc gcgagttcgc ctacgcggcg
     8341 tacaacgagg gcgcgtgggc ggatgcggtg cggctggcgc gtgagctggt gatgacgtgc
     8401 aaccagctca aggcgcagcg gcgagagccg gggcctcccc gctgaaatcg tccttccggg
     8461 tgacttgcga gaatgcgggt gctccggcga agtagcgccg caacatcgtc atcgagcgtg
     8521 agggctgtgc gcatacatct gaacatatcg acgtctacgc acttcgattt cggccccctc
     8581 agcgcgcctc cggcaggaag tagaacgcga tcccgagcac cacgtagacg gccagaagct
     8641 gcacaccttc gagccagttc gattcaccgt ccacagcaat ctggcctaca atgagcaccg
     8701 caagccccac cgccgccacc tccgcggggg tgaagacgag gttcagcggc cgcggcccga
     8761 taacgtaact cgccagcacc agcacgggcg ccacgaacag cgcgatctga atgctgctgc
     8821 cgatggcaat ccccagactc aggtccattc ggttctttag cgctaccagg atcgccgtgc
     8881 tgtgttcggc cgcgttgccg atgatcgcca ccacgaacac gccgacgaac acgctggtca
     8941 tgcccagtgc ctccgccgtg tgctcgacgc tccccaccag aatctcgctg atccacgcca
     9001 ccaacgccgt cgcccccagc agcacggaga tcgagcggcc ggcgctccaa accttgtgtc
     9061 ccccctgcac tcccgcggac tcggccgcgt tgccgacgaa aagctgcttg tgggtgtgca
     9121 gcgagaacaa caggctcaat ccgtaggtcg ccagcagtac gatcgagatc tccaggctca
     9181 ggctgccctc gcggacctcc atgcccggtc ccgccagatg gtggtagccc gccggtacga
     9241 tcaacccgat cgccgccagc gtcagcatcg tcgactgcga tcgcgccgcc accgcgttga
     9301 actgctgtga ccggtaccgc agcccgccgg cgatgactgc ggcgcccagc accagcagaa
     9361 tgttgccgat gatcgacccg gtcagcgacg ctttgacgac ctcgtgcagt cccttctgga
     9421 gcgccatcag ggcgataatc agctcggcgg cattgccgaa tgtcgcgttc aacaagccgc
     9481 cgatgccctc tcccgtgcgt tccgccaggt gttcggtggc cttcccaagc catcccgcca
     9541 gcgggagaat cgcaaggcag gccgcgaaga aaatcaggat gtgtgcgtca ggcagtagaa
     9601 accgcaaccc gatcgcaatc ggaacagata cgagcagcca gttcatgggg acggagtcat
     9661 tgtctcacgc aacaatgaat cggtcctcgt tcactggccg gctgccatcg atgcgaagaa
     9721 agcgtccgcc cgctttgaca tctcctcggg aatcacatcc tctttgtgcg tcgcaagacc
     9781 ccagtgatgg ccaaaaggat cgacgagctt gccatagcgg tcgccccaga acatgtccgc
     9841 cggaggcatc accggctttc cgccggcccc taccgccctt tgaaaagccg cgtcgacgtc
     9901 ttcgaagtac acgaacagtg acgctgtcgt cgtcccggcc gtctccggcg acgtgacatt
     9961 cgttcccggg aactcgtccg agaccataat cagcgaatcg ccgattttga tctcggcgtg
    10021 catcactccg tggccgccag ggccgggcat ccgcatgacc tcctgggcgc caaacgcccg
    10081 cttgtagaag tcgatcgcct ttgcggcgtc cttcactacc aggtatgggg tgacagtcga
    10141 gcagccgtca ggaattggtt tcaccatcgt tttgctcctc tgaagtgaaa gtctgaacct
    10201 ctctgaatgc ttgtgccgat gatacgcaag ctgccgcctg tgcgcaaccg ggcccccacg
    10261 aaccaccgta tcggctactc gggcttgagc gagcgttcca tcagttcccg gatcgcttcg
    10321 cgcggcggct tcccgtttcg cagcacttca tacatctggt tggtgatcgg aaggtcgacc
    10381 cccaattggc gcccgagttc gacggccgag taggttgtct tgacaccctc ggccaccatg
    10441 cgcatggagg cgacaatctc ttcaagccgg tggccccgcg ccagcttgat cccgacgctg
    10501 cgattccggc tcagttcgcc ggtgcacgtc aacaccaggt cgcccagtcc cgcgagcccc
    10561 gacaaggttg cggggcgcgc gcccaaggcc accgccaacc gggtcatttc caccagaccg
    10621 cgcgtgatca gggccgcgtt cgtgttgttg cccagaccca acccgtgaca gaccccggct
    10681 ccgatggcga tcacattctt cagcgatgcg gcgatctcga cgccgagcag gtcggtattc
    10741 gtatagaggc gcaggttggg tccggagaac gccgcctgga tctccgccgc cagcgccggg
    10801 tcttcggagg caatcaccac cgccgccggc tcgccccgcg ccacctcccg cgcaaacgtc
    10861 gggcccgaca acgccgcgac catcggctcg aaccggcgcc cggccacgtg gcggatcacc
    10921 tcggacatcc gctgccgcgt gccttcctcg agccccttcg tcgcgctgac gatcctcatc
    10981 gtgggcttca gatagggcag catctccgtg tagaggcgcc gcgcgtggtg cgagggcatc
    11041 acccccagca caatttccgc atcgcccaga gccgccgcga gctccgtctc cacccgtacg
    11101 ttcgccggca gttggaatcc cggcaggtaa agatcattct cccggagctt caccatccgg
    11161 gtggccagat cttgttcgta gatccacaag cgcacgcccg ggaatcgcgg tgccagcacc
    11221 agcgacagcg ccgtccccca gcttcccgcc ccaatgatcg ccagttcttt cacgtccgcc
    11281 gccctccaaa tgagaagacg ttttcgtttc cagcatgcaa gcgttggata ttcgacttgt
    11341 gccgccagat cacaaaagcc ccggccagca gcgccgctgc tgtcactgga gcctcggggt
    11401 gcgagatcag ccagaccgca agcggaaggc tccccgccgc gatgatcgat cccagcgaaa
    11461 tgtaccgggt gaccgccacc gtcaccgcaa agacaacgac cacggccgcc agcggcaggg
    11521 gagtgaggcg aaggaaaccg ccgacgcagc tagccaccgc tttcccgccc ttgaacttca
    11581 gcgccgcggg gaacgcgtgc cctaacatca ccgccagcgc ggcggcgctc gtccaaagcg
    11641 gcgaatcccc ggtcatccgc ccaaccagcc acaccgccac gtagcccttg gcgatgtcca
    11701 gcacaagggt cgtgatgccg gccgcgcgcc ccgccgtccg tagcacattg gtcgcgccga
    11761 tgtttccgct tccggacgtc cgcacgtccc tcccgctagt ccacttcacc agcaggtagc
    11821 cgaacgggac gccgcccagc aggtacgcga tcagcaaact caggattccg atggtcatcg
    11881 ttcgaaggga aattccgcga gacgtgtata aacggagcct gagggccgca tctcgctcag
    11941 gtacaggtag aaacggtctg ccgtaaaccg tccgaaatcg tcggattcca gccccgcgat
    12001 cgcctgccgc accgccgcca gcggaaccgg ctccttgatc cgtgccagtg tcaggtgcgg
    12061 cgcgaaaggt ttgggctctc tgggaacgcc gatccgggcc atcgccgcgt cagtctcgcg
    12121 cgcgaggtcg gcgagcgccg ccggcgctct caccgccgcc cagaacaccc gaggtgaatg
    12181 tgggttcgga aaccagccga gtccctgtac ggcgatctcg aaagcctcgc gctgcggcag
    12241 cgcggccaga gccggtttca gctcttcgag gcgttcttcc ggccattccc cgatgaactt
    12301 ggtcgtgatg tgcaggttgc ccggcgggct ccatttcagc ttcgccgtcg ggcgaagcct
    12361 gccgaccagc gtttccagat tacttttgac cgccgtggag atgtcgatcg cggtgaacag
    12421 gcgcatggtt gggaaaggaa aggctgtgat aatctccttc ccgtagccat tttcggacga
    12481 atacccccgc acttgcaatg agaaaaagag ccgaaatccc atttcgagac attggaaccg
    12541 ccgctggaat tctcccaaga tccttctaca gccgtccggc cgccgaagtg gcccgcgacc
    12601 tcctcggaaa gatcctggtc cacggccggg ccgcggggcg tatcgtcgag acggaagcct
    12661 acctcggcct gggcgatcgt gccgctcacg cctctcgcgg catcacgcct cggacgaagg
    12721 tcctgttcgg accgcccggg cacgcgtatg tctacttcat ctacgggatg tatgagtgcc
    12781 tgaacctcgt ggcggagccg gaaggcacgc cgggctgcgt gctgattcgc gcgctggagc
    12841 cgctggccgg tcttgcggcc atgcgccgcc gccgccccca tgcccgcacg aacgacgagc
    12901 ttgcttccgg gccggggaaa ctcacccttg cgatgggcat cacgcgccgt cacaacggcg
    12961 ctgacgtcac ctccggatcc ctcacagtcc gctcctggcc ggacgattcc cggttcgaaa
    13021 tcggcgtcag cccccgtatc ggcatccggc actgccgcga ctggccgctc cgcttcttca
    13081 ttgccggaaa cccctgcgtc agccgccacc ctgttgccgg tccttctgct agctgatact
    13141 ttcgacttat gctcaagccg accatcttcc gggaatacga tatccgtggt atcgccgaca
    13201 ctgagcttct tgatgacggt atcgagcaac ttggccgggc gatcggcagc tatcttcagc
    13261 gccggagcgg aaagagcatc aacttgggac gcgattgccg ccttagctcg acacgcctcc
    13321 ggaatgccct cgtgaaaggg ctcgtagcga gcggctgcga cgtcaccgac atcggcacgg
    13381 ttcccacgcc gctgctctac tactcggtct ttcaccttaa ggccacgggg gcggtgatga
    13441 tcacgggcag ccacaatcca cccgagttca acggcttcaa ggtgatggcc gggcccagca
    13501 ccatttacgg cgcccagatc caggaggtcc gcgagatcct cgagaagatg gatttcatcg
    13561 aagggcaggg aagcctccgg acggaggatg tctccggatc gtacatcgac caggtcgcgg
    13621 ccaacatccg gctcagccgc cgcgtgacag tggtctttga cgccggcaac ggcactgccg
    13681 gtcccatcat gcgccggctc cttgacagac tcaattgcga tcccgtcgag ctgtttttcg
    13741 acatggacgg caagttcccg aatcaccacc ctgatccgac cgtcgagaag aatctcgccc
    13801 atctcaaggc caccgtcgcc gcgcagaagg ccgagttggg catcgccttc gatggcgacg
    13861 ccgaccgaat cggcgccatt gacgagcttg gcaacgtcat ttggggcgac cagctcatgc
    13921 tgatttacgc ccgcgagatc cttactcgca agcccgggtc cacattcatc ggcgaggtga
    13981 aatgctcgca agtgatgtac gacgagctga agcggctcgg cggcaacccg attatgtggc
    14041 gcaccggcca ctcgctcatc aaggccaaga tgaaggaggc tcacgccgag ctggcgggcg
    14101 agatgagcgg ccacatcttt ttcgccgacc gctactacgg ctttgatgac gccctctacg
    14161 ccgcgtgccg cctgctcgag atcgtggccg actccggcaa gccgctttcg caccaactgg
    14221 ctggactgcc gaagatggtc tccactcctg agattcgcgt cgactgcccc gatgagctaa
    14281 agttcggcgt ggtcaatcgg gtcacggaac acttcaagaa ggttcgcgat gtcgtggatg
    14341 tcgatggtgt ccgcgtcctc ttcgaacagg gctggggcct cgtgcgggca tccaataccc
    14401 agccggttct cgtcttgcga ttcgaggctg ccaacggcgc gttgctcgca cagtaccagc
    14461 gcgaggtcga aacggtcctt gatgaagcga aggcggcctc aggggtgtga cgggcacccg
    14521 cgtaacgcgc ctcttcacac cgtcacgccg cgcaccatca acgccacaat gttccacacg
    14581 accagcagag cgtacatccc gttcgcccac gccagaaccc gctgccgtct cagccgccaa
    14641 caatagacag ccagcatcag tgcgagcgcc tttgccgcta ccaagcccgc cagcggagaa
    14701 cccgccgccc ccatcagcat ccggatcagc ggattcccct cctgtactcc atgcagaaga
    14761 aacgccagcg ttgtcaggat gtccagcact tgcaggtacg aaaactggat caggagggtc
    14821 gccacaaatc cgccttgcta aggaaaatta ccgagcatcc cgccggcaat tcccagctta
    14881 ggtaaggcga tccagactgt aaataccgga acgtaacacc gcacacctta gtacaggtgt
    14941 actaaggtgc gcctctccgc cgatcctgga catgggatcc attgtgactt tcaccgaaaa
    15001 ttctccacca gcatcaggtc gctgccgcct tcgtcgacct gcgtgtagag aatcgtccgg
    15061 ccgtcgggag aaaccgcgaa gaaggcaccg agcggtcgct cgatggtcag gatcttccgg
    15121 gtcttacctg tggcgaattc gtagattgaa aggtcactgt gccttttctt gtccgggacg
    15181 gtgaagaagt agattccctc tttgccaatg gtccacaggg ccaagggatg caccgcatcg
    15241 agaaccttgg ttccctcacc cccttcggcg ggcactcgcc agacgctctg cgggaacggc
    15301 catcccttgc tgtagtagac gaacttaccg tcgggagatt cgtgtggcag gtccgctcca
    15361 gaatcccggc taacctgcac cgcctctccc ccgccggcag gcatcttcca aatctcttgc
    15421 gaaccgccgc ggtccgacgt gaagtagagc cattggccat cacgcgacca atacggccac
    15481 ttgtctgcgc ccgggtcggc tgtcacacgc cgtggcatgc cgccggctgc actgacgaca
    15541 tagacgtctt gatttccccc cggttcccag gagaaggcca gcttctggcc gtccggcgac
    15601 caacttggtc cctggatcgc tggtcctcca agggaagtca gttgcactgc atcggatccg
    15661 tcggcgtcgc acacccagat ttctcggcca cccgaccggc tcgacacaaa ggcgattctc
    15721 ttgccgtctg gtgaataggc cggacccgct tcccaccacg tggaggagat gaacgggacg
    15781 ggtgcgcccg gcttgtgatt tgctccccgt aaatcgatcc gccagatgtt ggtgtcgaac
    15841 cttcccacca aatacgccag acggttcccc ttccgcgaaa ccgccggcgc gaaggcattg
    15901 tccggcgcga aaggaagcct acggggcgtg cctgggctgg ccgccgccag ccgccacaga
    15961 cctgcactgg aagcaaacac gatctctttt ccatccggtg tccaggccgg gctgaagctg
    16021 tccggatggt tcagtcgagc tctctgcggt tctcccagag gctggtaccc ctccccaaga
    16081 cggagcaggt ataactgcga ttttggacct acatagaaca gccgggcaaa ggccagggtg
    16141 tgaccgtccg gcgagaaggc aggagcagtg tcggttgacg gctcggcggg agggctggtc
    16201 agcttacgct tctctccggt ctcgacggag aacaggtaca aagccaactg tcccgcttcg
    16261 ggcagaggac aggcgagcca tttcgagtcc ggggtccagg ctaggtacgg cccgcccgaa
    16321 agggcgttcg tcgatccgga gaaatccaac tccgccaaca cccgttcgcg cccccctcgc
    16381 tgcgggatga ggaccaatga gaagctgctc gcatccggcg agagcccctg gaggaaggcg
    16441 atggtctgcc cgtcgggaga ccacgcgggg ctgaactcct gcgctggcgt atccgtcagt
    16501 ggggagggtg gctccacgcc gatttgcttg acgtagatgt cgcagttctt cccgctccag
    16561 aaaccctcgg gacaccgttg aaaggctacc tgcgtgccat cgggagaaaa actgggatac
    16621 ccctcttctc ctgggtagct ggtcagtggc actggaacga gcaccgcttc cgatgacctg
    16681 gggggagaac gcaagaacca aacgaggata agagagaccg cgaaga
//
LOCUS       DATIFQ010000215         5639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2177337, whole genome shotgun sequence.
ACCESSION   DATIFQ010000215 DATIFQ010000000
VERSION     DATIFQ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177337"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..2128)
                     /locus_tag="VLE22_10235"
     CDS             complement(218..2128)
                     /locus_tag="VLE22_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14829.1"
                     /translation="MSNSKGCLAVKTRPVDWIEQTSRRTGGEKPPSPRKRLGAFLILI
                     VLGSSVTVRAARGQEISFLREVFNDEPRGVAVDASGVYVVGGGFVRKFNVNGNNLWNR
                     SWPNSAGIVAEAGGVYVVGDAGLLRKYDTRGNQLWTQQIDAWPKAVAADVTGVYVGVY
                     TPGSGTNFVRKYTSNGVELWSRPLGKEGSWPTGMAVDASGVYVVAYHPDPGEDNFVRK
                     YTRDGAELWTRPFGQEGSWAVAVAVEATGIYLVGHTATGRILLGKYDSGGNELWIRQF
                     DSSNAGFTAASGVAADATGAYVVGRTNVALPEQCRSGSQDAFVRKYDANGASLWTRQF
                     SGSSDSGWANGVAAHATGLYVAVGSRGKGLLAKLEKMQPVIGESGPWISPGCVVNAAS
                     YRGGRISPGEIVTVFGRRIGPPQATPLRLTEGRRLATELAGTRLQFNGIAAPLLFVSA
                     DQISAVVPYAVAEKLTADVEVEYQGVRSNKVTMPVAASRPGVFTWDRSGSGPAAALNE
                     DGRINSDSKPARRGSAIVLYATGEGLTKPVVEDGLILGSVLPKPNLPVSVSFLTPFCQ
                     VLNEYDCHDVYVKGEVLYAGGAPESVAGLLQVNVRVPSGVYVDGKGRLWFKLCVDQRC
                     SEEQVNHVFFKP"
     gene            2493..4406
                     /locus_tag="VLE22_10240"
     CDS             2493..4406
                     /locus_tag="VLE22_10240"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HSB14830.1"
                     /translation="MAGNIVHSIVRDSRGLLWFCTNDGLSRFDGHNFVTFGSEHGLGD
                     QEVFDFLETSNGVYWVATARGVSRMNPRTTSGAAVSFKNFLPGEDRPSQWTLRLLVAH
                     DGRIWIATNSGLYRMSVEAGQGPVFQHVPLPSPMEPESSHRVLALVEDGFGQIWAGGD
                     SGLFRVVDGESVERYSIADGLPGNTVLSLALDSSGALWVGVHGGFCRLTPRKLGHAVV
                     DRVHKLSAGKPDDWVAAMHATSEWLWVGTVDGLLRLPVAADRDLQLFGLANGLVHRSI
                     ESLAEDQQGSLWIGSMDSGASRLSTEGFTTYGTDDGLASVEIAGIMETRAGELVAISK
                     GLYQFSLNWFDGKRFLAIRPRFPPSVTGFGWGWAQVAVQGPSGEWWIATREGVVGYPY
                     ASAGQLAFSEPRAFLRTSRELPPGNVFRVFADSRGDLWISTSDDRSHGLARLERRTGR
                     IQHFRQEDGLPGPAGDDRSLASAFAEDASGTVWIGFHRGGLFRIRDGRLLPVRPHQDA
                     EIEGVRWIHRDAVGRLWVAGRKGLLRVDEPTAERPVFRHYGVAQGLRSSFVLSIAEDH
                     LGRIYLGSGHGVSRLDPATGSVHHYGSADGLVGGEIRVAYRDRRGYLWFASTEGLSRY
                     DPRQEQHPPAPNV"
     gene            4536..5432
                     /locus_tag="VLE22_10245"
     CDS             4536..5432
                     /locus_tag="VLE22_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB14831.1"
                     /translation="MRYQYQLENVDKSWSEAKEWRHVDYPGLGAGSYRFLVRAVNVEG
                     TWSLHPAAVHFQIEPPFWASWWFRLGVAAITLAAVIFLHRVQVAKAVAVERIRHRIAK
                     DLHDDVGASLCNAALLSDLMNREEQVQSAMGRERLGKIGSICREALESMSNIVWAIDP
                     QKDKVSDMMERMRSFGEETLGPQGLELTFSGDSASDIVLHPNVRHQMFLIFKEAIHNV
                     VRHSSATGVRVELSTKAHRLTLRIADNGRGLPPGQGDYGHGLRNMRERITSLGGTMRV
                     DTGSNERGTMLEFTVPLTKVRR"
     gene            complement(5445..>5639)
                     /locus_tag="VLE22_10250"
     CDS             complement(5445..>5639)
                     /locus_tag="VLE22_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSB14832.1"
                     /translation="HNLTSHELRLLRLLVEGHSYRTAAVGLGVTVHTVSFHLRRIYDK
                     LHVHSKSEAVVKALKDGLVR"
BASE COUNT         1077 a         1706 c         1709 g         1147 t
ORIGIN      
        1 ctcggcagcg gttgcttttc cccgcccgtc cagcggtaaa tttgttcgat ccaatcaact
       61 gggccggtct tcagtgccaa acaacctttg ctgttgctca tcttgtcctc ctcgcagcct
      121 tagcggggtg caggctggat ggccaactcg ggcccccgcg catcttgatt cccggcgacg
      181 cgaagcttgt gagtcacggc ctcccccgct atcgccgtca gggcttgaag aacacgtggt
      241 tgacttgctc ctctgagcac ctttgatcca cacagagctt gaaccacagc ctacccttgc
      301 cgtcgacgta aactccggat ggcactcgca cattcacctg aagcagcccg gcgaccgact
      361 cgggcgcccc tccggcgtat agaacctcgc ccttgacata cacatcgtgg caatcgtact
      421 cgttaaggac ttggcaaaaa ggagtgagaa acgatactga gaccggcaga ttcggcttcg
      481 gcagcaccga accgagaatc aagccatcct cgacgaccgg ctttgtcagc ccttcgccgg
      541 tggcgtacag aacaatggcg gagcctcttc gtgccggctt cgaatccgaa ttaatcctcc
      601 catcttcgtt cagggctgcg gcaggtccgc taccggaccg gtcccaggtg aaaacgcccg
      661 gacgcgaagc tgccaccggc atcgtcactt tattcgagcg caccccctgg tattcgacct
      721 caacgtccgc cgtgagtttc tcggccaccg cgtacggaac gacggcgctg atctggtcgg
      781 ccgacacgaa gaggagcggc gctgcaatgc cgttgaactg gaggcgggtg ccggccagct
      841 cggttgcgag ccggcggcct tccgtaaggc gaagcggcgt cgcctgcggt ggcccgatcc
      901 gacgtccgaa aaccgtcacg atctcaccgg gggaaattcg gccaccccgg tagctggcgg
      961 cgttgacgac gcagcccggc gagatccagg gccccgattc gccgatcacc ggctgcattt
     1021 tctcgagctt ggccagcagg ccttttccgc gtgaaccgac agcgacatag agacccgtgg
     1081 cgtgcgcggc cacaccgttc gcccaaccgg agtccgacga gcccgaaaac tggcgggtcc
     1141 aaagcgacgc gccattggcg tcatatttgc gtacaaaggc gtcttgggaa ccggacctgc
     1201 actgttcggg gagggcgacg tttgtccttc ccactacgta ggcgccagtc gcatccgccg
     1261 ccacgccgct cgccgcagtg aagcccgcgt ttgacgagtc aaactggcgg atccaaagtt
     1321 cgtttcctcc cgagtcatac ttgcccagga gaatgcgtcc cgtagcggtg tggccgacca
     1381 agtaaatgcc cgtcgcttcc accgccacgg cgactgccca ggagccttct tgcccaaacg
     1441 gacgggtcca cagttccgcg ccgtcccgag tgtactttcg cacgaagttg tcttcgcccg
     1501 ggtcgggatg gtaggcgacc acgtagacgc cggaagcgtc cacggccatg ccggtaggcc
     1561 aggagccttc tttcccaagc ggacggctcc acagttccac gccgttgctg gtgtacttcc
     1621 gcacgaagtt ggtgcccgag ccgggagtgt agacgcccac gtagacgcct gtgacatcgg
     1681 ctgccacggc cttcggccag gcgtcgatct gttgggtcca cagttggtta cctcttgtat
     1741 cgtacttgcg aagcaagcca gcatcgccaa cgacgtacac cccgccggct tctgccacga
     1801 ttcccgcgga gttaggccag gatcggttcc acaggttgtt gccgttgacg ttgaacttgc
     1861 gcacgaaacc gccgcccacc acgtacacgc cactggcatc caccgccaca ccccgaggct
     1921 cgtcattgaa gacttcccgc aggaaggata tctcctgtcc tcgggcagcg cggacggtga
     1981 ccgacgaccc gagaacaatc aggatgagaa aagcgccgag gcgtttcctc ggcgacggcg
     2041 gcttttcccc tcccgtccgg cgggaggttt gctcgatcca atcaactggc cttgtcttca
     2101 ctgccaaaca acctttgctg ttgctcatcg cgtcctcctc gtagacttaa tggggcgcgg
     2161 gctgggtaac caactcgggt agcccgtgca tcttgatgaa aatgcccgtc aaacccccgt
     2221 cgccgcatac cacaaccgtc gcctgagatt agtggaccga ttagtgccgc caccagcagt
     2281 gttaggctcc tgcctggaga atacccccac ttgctcaccg acccactaca caaacgcgta
     2341 gtcttttctc gtatactcct gggaagaggc ggaaggcgat gcgtcatccg tctgggtcga
     2401 gattagcggt tctactgtgg ggtgccctcg tcgcgtgcgg catcgctcgg gcggagaaac
     2461 taccctttcg gcatttcacg acgcaagaag gcttggccgg taacatcgtg cacagtattg
     2521 tgcgcgactc acggggcctg ctctggttct gcaccaatga cggcctgtcg cggttcgacg
     2581 gtcacaattt tgtgacgttc ggctcggagc acgggctggg cgaccaggaa gttttcgatt
     2641 ttctcgaaac ctcgaatgga gtttactggg tcgccaccgc gcgcggggtc tcacgaatga
     2701 atccgcgcac gacatccggc gccgccgtct cgttcaagaa cttccttccg ggagaggaca
     2761 gaccgtctca atggacgctc agactgctcg tggctcatga cggacgcatc tggattgcta
     2821 ctaatagcgg cctctaccga atgagtgtcg aggcagggca gggtccagtc tttcaacacg
     2881 ttccattgcc atcgccgatg gaaccagaat cctcgcaccg cgtccttgcg ctggtcgaag
     2941 acggcttcgg acagatctgg gcgggcggcg acagcggcct ctttcgagtc gttgatgggg
     3001 agagtgtcga gcggtattcc atcgcggacg gccttcccgg caacacagtt ctttcgttgg
     3061 cgctcgatag tagcggagct ttgtgggtag gagttcacgg aggtttttgt cgcttgacgc
     3121 cgcggaagct cgggcacgcc gtcgtggacc gtgtacacaa actcagcgcg ggtaagccgg
     3181 acgactgggt ggcagccatg catgcaacaa gtgaatggtt gtgggtcgga acggttgacg
     3241 gtctgcttcg tttgccggtg gcagccgacc gcgacttgca gttgttcggg ctagcaaatg
     3301 ggctcgtcca tcgcagcatt gaaagtctgg cggaagacca gcaaggcagc ctatggatcg
     3361 gaagcatgga tagcggagcc tcgcggctct cgacagaggg cttcaccact tatggtactg
     3421 acgatggcct cgcttccgtc gagatcgccg gcattatgga aaccagagcc ggggagctcg
     3481 tcgcgatctc taagggccta taccagtttt ctctgaattg gttcgacggc aagcgcttct
     3541 tggcgatccg gccacggttc ccgccgagtg tgacggggtt tggctggggc tgggcgcagg
     3601 tggccgtgca gggcccttcg ggcgagtggt ggatcgcaac ccgcgagggg gtggtaggct
     3661 acccgtacgc atctgccggt caactcgctt tctcagagcc ccgcgctttc ctccgaacgt
     3721 cccgagaact cccaccgggc aacgtcttta gggtgttcgc agatagccgg ggcgaccttt
     3781 ggatcagcac gtcagacgac cgctcgcacg gactcgcgcg attggagcgc cgaaccggac
     3841 gcatccaaca tttccgtcag gaagacggcc tgcccggtcc ggccggagac gaccgcagtc
     3901 tggcttccgc cttcgccgaa gatgcctccg gaacagtttg gatcggattt caccgaggcg
     3961 ggctcttccg gatacgtgac ggccggctgc ttcccgtccg cccgcatcag gatgctgaaa
     4021 tcgaaggcgt gcgctggatc caccgcgatg ccgtcgggcg tctttgggtc gctggccgca
     4081 agggtcttct acgcgtagac gagcccaccg ctgagcggcc cgtgtttcgc cattatgggg
     4141 tagcgcaagg gctcagaagc agtttcgttc tgtccatagc tgaagatcat ctgggtcgaa
     4201 tctatcttgg gtcaggccat ggcgtaagcc gcctcgatcc ggctacggga agtgttcacc
     4261 attacggatc cgccgatggc ttggtgggtg gtgagatccg tgtggcctat cgcgacaggc
     4321 ggggatatct ttggttcgcc agcaccgagg gcctcagccg gtacgatccg agacaggaac
     4381 agcaccctcc ggctccaaac gtgtagatct cgcggcttcg cattggaagc tatgaacagg
     4441 agctggcgcc cgctggtgag ccacgggttt cagtcgcacc gctaagctgg aaggacaact
     4501 ccttgcatgt ggagttcttg ggagtgggag aaaggttgcg ctatcagtat caattggaga
     4561 atgttgacaa gagctggtct gaggctaagg agtggcggca cgttgactat cccggcctgg
     4621 gagctggcag ctatcgcttc ctggtgcgcg cggtgaacgt tgagggaacc tggagcctgc
     4681 accccgcagc cgttcacttt caaatcgagc cgccgttttg ggcaagctgg tggtttcggt
     4741 tgggagtcgc tgccatcacc cttgcagccg tgattttcct ccatcgagtt caagtcgcca
     4801 aggcggtggc ggtagagcgg attcgacacc gaattgcgaa ggatcttcat gatgatgtag
     4861 gcgcaagcct ctgcaatgcc gcgctgctga gtgacctcat gaaccgggaa gagcaagtcc
     4921 agagcgccat gggccgtgag cgccttggca agattggctc catctgtcgt gaggcgttgg
     4981 agtcaatgag caatattgtc tgggcgatcg atccgcaaaa ggacaaggtg agcgacatga
     5041 tggagcgcat gcgcagcttt ggcgaggaaa cactgggacc ccaagggctg gagcttactt
     5101 tctcaggcga cagcgcttcg gacatcgtgc tccatcccaa tgttcggcat caaatgtttc
     5161 tgattttcaa ggaagccatt cacaatgtcg tgcggcactc aagtgcaacc ggcgttcgtg
     5221 ttgagctgtc caccaaggct catagattga cattgcgaat agccgacaac ggccgcggtt
     5281 tgccgcctgg ccagggtgat tacgggcatg gacttcgcaa catgcgtgaa cggatcacat
     5341 ccctcggggg caccatgcga gtagacactg gctcgaacga acggggcaca atgttggaat
     5401 ttacagttcc cctcacgaag gtccgacgct aacgaactcc ctcgtcatcg aacaagtcca
     5461 tctttgagtg ccttgactac ggcctccgat ttcgaatgca cgtgaagctt gtcgtagatc
     5521 cgccgcaggt ggaacgacac tgtatggacg gtgacgccaa gtccgaccgc cgcagtccgg
     5581 taactatgcc cttccactag caaccggagc agtcgcaact cgtgggacgt caggttgtg
//
LOCUS       DATIFQ010000216         6167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1603201, whole genome shotgun sequence.
ACCESSION   DATIFQ010000216 DATIFQ010000000
VERSION     DATIFQ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603201"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VLE22_10255"
     CDS             <1..1059
                     /locus_tag="VLE22_10255"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14833.1"
                     /translation="TVVEEVFLKNSEHPGALHYLIHSYDDPIHAPLGLRSARIYARVA
                     PQAAHALHMPSHIFVAMGMWDEVIASNEDSWAASEARVKRKNLPLEDRGYHSLWWLQY
                     GYLQQGRFEDARRALGIVEQDAKASPSPLIRFHLVQMCAVYSIETGRPCELPSGITTS
                     GLDLPAVAANLLATGMANLNRGQRAGAESSLSELRKLKGALHPGSSETQMSHHDHIYP
                     GDVQAVEIMKKELEALLLTADGRSKDAVQLMVEATALEDKMSFEFGPPVPPKPSHELF
                     GEILLQLERPDAARAQFEMALIRAPGRALSLLGLARAHAQMGEKAKARETYTELRRMW
                     RRADKEIQKALDDTLAKM"
     gene            complement(1110..4535)
                     /locus_tag="VLE22_10260"
     CDS             complement(1110..4535)
                     /locus_tag="VLE22_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB14834.1"
                     /translation="MAQGVNGRKPSFLPNALFVSLATLTVLAAPAEAQVLYGSLVGNV
                     TDESQGAIRGAEVTIANRETNLTRKALTNELGLYSFPNVPSGTYSVRVTMPGFADFVR
                     ADVPVAVNSAVRVDAVLKVSGVASEVTVTAGAEVAPLQTERADVRHEISRKQFEDLPA
                     PLGRNYQNLLVVIPGFELAGSVREGRLAGCNPSRSRAFNVNGTTRSTTVTNVDGASNA
                     HIWNVGSSVIIPNLEAIEDVNVTTNSFDAEQGLAGGAVINVQTKSGGNQFHGTAYEYH
                     SNQHLKARDYFRPAGQEKGKYITNQFGGTLGGPIKRDKLFFFTALEGRAERENASATF
                     SLPSPAVRSGDFSGFNTVIYDPLTGNPNGSGRTPFDGNRIPEDRIDPISRKVIDLVPL
                     GNLPGLLDTDSNNYFASNPFKGNRWSTDNKVNWQASDNLNTFIGWSYFHYRAINPTAY
                     GEGFIDGPRVGGGNAGESWGYNNRISAGATYIFSPTLLVDGFFGWTRQNTNVEQPGIG
                     TNYGSEELGIPGTNGPERFQSGWPQFNVSGYAVFGTEEPWTPYYRNDNQYSFRSNFTK
                     TRQSHEIRWGVDVNSEQMNHKQPEFQGGASMGARGRFNFGTGPTSACLTVSATGGCSR
                     VSPVITQANAFASFLLGLPNSHGKNLLAVFPYSTRVWRYSLYLRDRWQVNQKLTLSYG
                     VRWEYFPMPTRADRGFERYNPDDNMMYIGGVGDVPRNLGVEVSKKLFAPRFGLAYRAT
                     DTFVVRAGYGISYDPYSLSRPLRTNHPILIELVVPSANSLSSSGQLQNGIPPIVPPSL
                     DDGIIPIPSNVSAQTLPLKDQRGYVQSWNLMLQKRLMWGFVGEAGYVATRQIRQLSYM
                     ELNWAPIGGGTAGQQLNKKFGRTANTRQVTPVGGSHYDSFQATLQRRFAGGYSFYAAY
                     TLGKSISNSGLDTSDSTLAIPIPEYYGLNRSVNGFDRRHNLQITNIADLPFGKSRRWL
                     NDRGALSAIVSGWQINSIISVMSGRPFSITASGSSLNAPFSTQRADQVKPEVQILGGV
                     GRGQPYFDPTAYAAVSEPRFGTAGFNSLYGPGRVNWDLGIFRQFQLTEKMNLQFRLES
                     FNFTNTPKFGNPGNNVSNLQFNQDSSIRNLNGFGEITSASEERQFSLGLRLSF"
     gene            4872..5459
                     /locus_tag="VLE22_10265"
     CDS             4872..5459
                     /locus_tag="VLE22_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14835.1"
                     /translation="MILKLDQAVKERHRFSLELASSNGFAGPARIFSTAGDSAAPVRH
                     IEDRRAVLEHIFTLSPNTVNTANFTVTRDVVRSGEKEDRLGYPDRIGLKGASAPFFPV
                     ISFQPYVSMGRLYPANTSASNIYTWTDALSVRRGKHTIKLSAEYTGRQLNIYNPQYPS
                     SLLRFSAGLTSLPGIVNTGTGTLPPASPRPSEPSF"
     gene            5483..6094
                     /locus_tag="VLE22_10270"
     CDS             5483..6094
                     /locus_tag="VLE22_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14836.1"
                     /translation="MLYGVFLAAAREEYAKAAAEAKLALDLAPLSQYVNTSAAWVHVF
                     VDNHAEAIEQARGALELFPESLQGYYTLGLAVLGRGGYEEAISVFEKAAAISPDAISI
                     SYLGHAQARAGHVDAAIRLTGELLARSEREHIPPRTLAILYTGLGDFDRAFEWLERAF
                     RARDSGLLWLRVLPLYDSLRSDPRFEGILRRVGLLSGDSGQAA"
BASE COUNT         1218 a         1806 c         1898 g         1245 t
ORIGIN      
        1 accgttgttg aggaggtgtt tctcaagaat tcggagcatc ccggcgcact gcattacctg
       61 atccattcgt atgacgatcc gattcacgcg cccttgggat tgaggtcggc gcgcatctat
      121 gcccgagtcg caccgcaagc cgctcatgcg ctgcacatgc cctcccacat cttcgtggcg
      181 atgggaatgt gggatgaggt aattgcgtcg aacgaagact catgggccgc ctccgaggcc
      241 cgagtgaagc gaaagaacct gcctctcgag gaccggggat accattcgct ctggtggctt
      301 caatacggct accttcagca aggcagattc gaagatgcgc gacgtgcgct cggaatcgtg
      361 gagcaagacg cgaaagcgag cccttccccg ctgatccggt ttcatctggt gcaaatgtgc
      421 gccgtctact cgatagaaac cggacgtccc tgcgagttgc ccagcgggat caccacctcc
      481 ggcctggatc taccggctgt ggccgcgaat ctgctcgcga cgggaatggc gaatctcaac
      541 cgtgggcagc gggccggcgc ggaaagctca ctctccgaac tgcgaaagct caagggggcg
      601 ctacatccag gatcctcgga aactcagatg tcgcatcacg atcacatcta ccccggagac
      661 gttcaagccg tcgagatcat gaagaaggaa ctcgaagcgc tgctcctcac ggcggacggg
      721 aggagcaagg atgcggtcca actgatggtg gaggctacag cgctggagga caagatgagc
      781 ttcgagttcg gcccgcctgt tcctccgaag ccttcccacg agttgttcgg cgaaatcctc
      841 cttcagttgg aacggccgga tgcagcccgt gcccaattcg aaatggccct gatccgggcc
      901 ccgggaaggg cactttctct gctcggcctg gctcgcgccc atgctcagat gggtgaaaag
      961 gccaaagctc gcgaaactta caccgaactg cgaagaatgt ggcgccgcgc ggacaaggag
     1021 attcaaaaag cgctcgacga taccttggcg aagatgtgag ggcggttctt ctgcttcctc
     1081 accctgcagt tgttcgcccg cggcgaatgt cagaagctca gccggagtcc gaggctgaac
     1141 tggcgctcct ccgacgcgct tgtgatctcg ccaaagccgt tgaggttccg aatggagctg
     1201 tcctgattaa actgcaagtt gctgacgtta ttgccgggat tgccgaactt cggcgtgttg
     1261 gtgaagttga aggactccag acgaaattgc agattcattt tttcggtcaa ctggaattgg
     1321 cggaagatcc cgagatccca attcaccctc cccggaccgt agagtgagtt gaagcccgcc
     1381 gtgccgaacc gaggctcgct caccgcggcg tacgcggtcg ggtcgaaata gggctggcca
     1441 cggccaactc cgccaaggat ctggacttcc ggcttcacct ggtcggcccg ctgtgtgctg
     1501 aagggggcgt tcagggaaga gccggaggcc gtaatcgaga atggccgccc gctcatcaca
     1561 ctgatgatgc tgttgatctg ccaaccgcta actattgccg aaagagcccc gcgatcgttc
     1621 agccagcggc ggctctttcc gaacgggaga tccgcgatgt tggtgatttg caggttgtgt
     1681 cgccggtcaa aaccgttcac ggaacggttg aggccatagt actcgggaat gggaatcgcc
     1741 aacgtgctgt cgctggtatc caacccggaa ttgctgatcg atttgccgag agtgtaggcg
     1801 gcgtagaaag agtatccgcc ggcgaagcgc cgctggagag tcgcctgaaa ggaatcgtag
     1861 tggctcccgc cgacaggcgt cacttgacgc gtgttcgcag tgcggccgaa cttcttgttc
     1921 aattgctggc ccgcggttcc cccgccgatg ggggcccagt tgagttccat gtaactcaac
     1981 tggcgaatct ggcgggtggc gacgtaaccc gcctcgccca caaagcccca cataagtctc
     2041 ttctgtaaca tcaagttcca cgactggaca tagccgcgct gatctttcag gggaagggtt
     2101 tgcgcggaaa cattcgaggg gatggggatg atgccgtcat ccaagcttgg cggcacgatg
     2161 ggcggaattc cgttctgcag ctgcccagaa gaagacaggc tgtttgcgga cgggacaacc
     2221 agttcaatca gaatggggtg attcgttctc agaggccggg acaacgagta cgggtcatag
     2281 ctgattccgt acccggcgcg caccacgaaa gtgtcggtgg cgcgataggc gagcccgaag
     2341 cgcggcgcaa acagtttctt gctcacttca actccgaggt tcctgggcac gtcaccgact
     2401 ccgccgatgt acatcatgtt gtcgtccggg ttatagcgct cgaacccgcg gtccgcccgg
     2461 gtcggcatcg ggaagtattc ccaccgcaca ccgtacgaga gcgtcagttt ctggttgacc
     2521 tgccagcggt cccgaagata gagactgtaa cgccaaacac gagttgagta agggaagaca
     2581 gccagcaaat tcttgccgtg cgaattcggc aacccaagca gaaaagaggc gaatgcattc
     2641 gcctgcgtga tgacggggct caccctgctg caaccgcccg tcgcgctgac cgtgaggcac
     2701 gcgctggtcg gaccggtacc gaagttgaac cgcccgcgag cgcccatcga ggctcctcct
     2761 tgaaactcgg gctgcttgtg attcatctgc tcgctgttga cgtcaacccc ccagcggatt
     2821 tcgtggctct ggcgggtctt cgtgaaattg gaccggaagg agtactggtt gtcgttccga
     2881 tagtaaggag tccagggctc ttcggttccg aacaccgcgt aaccgctcac gttgaactgc
     2941 ggccagccgc tttggaatct ctccggaccg ttcgtgcccg ggattccgag ttcctccgaa
     3001 ccatagtttg tgccgatgcc gggctgttcc acgttcgtgt tctggcgggt ccacccgaag
     3061 aaaccgtcca ccagcaaggt cgggctgaag atgtaggtcg ccccggcgct gatccgattg
     3121 ttgtaccccc atgactcgcc ggcatttcct ccgcccacgc gcggcccgtc gatgaacccc
     3181 tccccgtagg cggtcggatt gatcgcccgg taatggaaat agctccagcc gatgaacgtg
     3241 ttgagattgt cgctcgcctg ccagttcacc ttgttgtccg tggaccagcg gttgccttta
     3301 aaagggttgg aggcaaaata gttgttgctg tccgtgtcca gcagtccggg aaggttgcca
     3361 agtgggacca gatcgatcac tttccgggag atggggtcga ttcgatcctc gggaatcctg
     3421 ttaccgtcga agggagttcg tccggagccg ttgggattac cggtcaacgg gtcatagatc
     3481 acagtgttga aaccggaaaa atcgccgctt cggacggccg gtgagggcag agagaaagtg
     3541 gcgctcgcgt tctcccgttc ggccctgcct tcaagggcag taaagaagaa caacttgtct
     3601 cgcttaatcg gaccgcccag cgttccgcca aactggtttg taatgtactt gcccttttcc
     3661 tgtccggccg ggcgaaagta gtcgcgcgct ttcagatgct ggttgctatg gtactcgtaa
     3721 gcggtcccgt gaaactgatt gcccccgctc tttgtctgaa cgttgatcac cgcgccacca
     3781 gccagtccct gctcggcgtc gaaactgttg gtggtcacgt tgacatcctc gatcgcttcg
     3841 agattgggga tgattacgct cgacccgacg ttccagatgt gagcgttcga tgccccgtcg
     3901 acgttcgtga cagtggtgga ccgggtggta ccgttcacgt tgaacgccct ggagcgcgag
     3961 ggattgcagc cggcgagacg gccttcgcgc acggaaccgg ccaactcgaa acccgggata
     4021 acgacgagca ggttctggta attccggcct aacggcgccg ggagatcttc aaattgcttg
     4081 cgcgagattt cgtgcctgac atcggcgcgc tcggtctgaa gcggagcgac ttcggcgcca
     4141 gcggtcacgg tcacttccga ggcgacacca gagaccttga gcacagcgtc aacgcggacg
     4201 gcactattta ccgccactgg cacgtcggct ctcacaaagt cagcgaaccc cggcatcgtc
     4261 actctgaccg agtaagttcc cgacgggacg tttgggaaac tgtatagacc gagttcgttg
     4321 gtcaaagcct ttcgagtcag gttcgtctct cggttggcga tggtcacttc ggcgccgcga
     4381 atcgcccctt gggactcatc ggtgacgttg cctaccagcg atccgtagag aacctgcgcc
     4441 tcagcaggcg cagccaagac agtcagcgtg gccagtgaaa cgaacagcgc attcggaagg
     4501 aaggaaggct tcctcccgtt gaccccctga gccataccgc accccctcaa ttcagaagtc
     4561 cctctattac ccagatgttc cagacacctc aaccgtcggc attcacccta ttcccagtgc
     4621 acatttgggc gattgaggtt aaggatggtt tatcatgaga actctgattc tgtcaaggtt
     4681 tttctttggc tgaccgacgg accctaatct gcccatagtg ttacgcacga gctgagctga
     4741 aggcgcttgg ctgaaagagc atatcgtgaa taggcgcggg ggaatgattc gccgatggct
     4801 cgcggcagcc gcgttgccgg ctgcgtgctt ggcgggaatt gcgtcggccg agccgcaaca
     4861 cgccggatgg aatgatcctc aagctggacc aggcggtgaa ggagcggcac cgattcagtt
     4921 tggagctggc aagttccaac ggattcgccg gcccggcccg aatcttttcc acagctgggg
     4981 attcggcggc gccggtgagg catatcgagg accggagggc ggttctggag cacatcttca
     5041 ctctatcgcc gaataccgtg aacacggcca acttcactgt gacgcgggac gtcgtgcgat
     5101 ccggcgagaa ggaggacagg ctgggttacc cggatcggat cgggctgaag ggcgccagcg
     5161 ctccgttttt tccggtaatt tcctttcagc cgtatgtctc gatgggccgg ctgtatcccg
     5221 cgaatacgag cgcgagcaac atctatacgt ggaccgatgc gttatccgta cgccgtggca
     5281 aacacactat caagctgtcc gcggagtaca cgggcaggca actcaatatt tacaacccgc
     5341 agtacccgtc gagcctgttg agattcagcg ccggccttac gagtttgccg gggatcgtca
     5401 acaccgggac tgggactttg ccacctgcga gtccgaggcc gtccgagcca tccttctgag
     5461 cccgagcgac gcgggcgccc atgtgctgta cggagtgttc ctcgcggcgg cgcgcgagga
     5521 gtacgccaag gccgccgccg aggcgaagct ggctctggat ctggcaccgc tatcgcagta
     5581 cgtgaatacc agcgcggcgt gggttcacgt gttcgtggac aaccatgcgg aagccatcga
     5641 acaggcgcga ggggcgctag aacttttccc ggagtcgcta cagggctact acactctggg
     5701 actggccgtc ctgggtagag gcggatacga agaagcgatc tccgtcttcg aaaaggctgc
     5761 cgcgatttct ccggacgcga tctcgatatc gtatctggga catgcccaag cgagggccgg
     5821 ccacgtggac gccgcgattc ggctcaccgg cgagttgctg gcgaggagcg aacgggagca
     5881 catcccgccc cgaacccttg cgattctgta cacgggactt ggagatttcg accgggcctt
     5941 cgagtggctg gagagagcct ttcgggcgcg cgattccggg ctgttgtggc ttcgcgtcct
     6001 ccccttgtac gattcgctgc ggtccgaccc ccgtttcgag gggattctgc gcagggtcgg
     6061 gcttctgagt ggcgactccg gccaggcggc ctgagaggcc gccgcaggcc ggggtcctgc
     6121 cccacactga ggcggccacg gaaccggaag cgggttcgga agccccg
//
LOCUS       DATIFQ010000217         3069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1603601, whole genome shotgun sequence.
ACCESSION   DATIFQ010000217 DATIFQ010000000
VERSION     DATIFQ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603601"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VLE22_10275"
     CDS             <1..328
                     /locus_tag="VLE22_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14837.1"
                     /translation="AEMWGNNVPALILEGGLGSVFAVLLVYLVKKLGIGDVLLWALVW
                     CTRVFASLNGLRRLPEASPELPTYLGLQACSAIALIVVLLRSEFRIFHEAIVRAFTIQ
                     LLWRGK"
     gene            complement(446..643)
                     /locus_tag="VLE22_10280"
     CDS             complement(446..643)
                     /locus_tag="VLE22_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14838.1"
                     /translation="MKYRVMSRAMDLFSASLDKVGDSLHFRIETVQFLAKFAQPSVKG
                     FLRVCRHASLDELAEELGNTS"
     gene            627..1607
                     /locus_tag="VLE22_10285"
     CDS             627..1607
                     /locus_tag="VLE22_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HSB14839.1"
                     /translation="MTRYFIGSLLLAAGCLSAEQPAAQQPDNSPAGQNPIHSSYVLGP
                     DDQLSIRALDAPEITDKPLRISTSGQISLPMVGRLRAAGLTVDELERQLSDRLRVYVR
                     DPQVAISVTEFRSQPVSVIGAVNQPGIHQLQGTKRLIEVLSLAGGLRNDAGNTVTITR
                     RKEWGEIPLPNVKPDASGEFTIADVSVKSIMAAENPKANISILPNDVITVPKADLVYV
                     IGSVRRSGGFVLGERETISVLQALSLAEGLDRTSAPRNAKILRLSGNDNKDRSEIAVD
                     VKKILEGKTGDVWMQANDVLFIPGSAAKSATLRGIEAAIQVGTGVAIWRR"
     gene            1656..>3069
                     /locus_tag="VLE22_10290"
     CDS             1656..>3069
                     /locus_tag="VLE22_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HSB14840.1"
                     /translation="MKQIPSLPPGREPVEITPSNGHGQPSKFGYIEAQGYTYAQGYPA
                     EEADAPGLVEYSRILRRRKGTLILVSFLGLSAAVLITVPQTPIFQSRTAIEIQDINDD
                     FLNIKQVTPVQDAPSTGHLTDIQTQVKIIQSETLRERSANLLKKKQPASFNARRGRFA
                     AWRELLRLPKSSPMTPYELEVENIRKEVQVKPSGQTRIVEVLCDSTDRNLAAAFCNTM
                     VSEYIDQNMEARWTMSERTGEWLSRQLAEMRVRLERSEDGLQNYARQSGLLFTSEKEN
                     VSDEKLRQLQAALSAAQADRVAKQSRYEIMRTVSADTLSDAVPDGSLNFLVTRLADLR
                     RQEAELTTTFTTEHSSVKRVRAQIASLEKTLNEQRGIMLNRIRNDYEEALKREKLLGA
                     AYASQSRLVTQQAEKSIQYNILKREVDSNRQLYESMLQRVKESSIVSAMRASNVRVVD
                     MARPAKSPYKPRLALNSLLGL"
BASE COUNT          657 a          948 c          939 g          525 t
ORIGIN      
        1 agcagagatg tggggtaaca acgtacccgc tttgattctg gaaggcggcc taggatcggt
       61 cttcgccgtc cttctcgtgt atctcgtcaa gaaacttggg atcggagacg tgctgctttg
      121 ggctctggtc tggtgcacgc gcgtgtttgc gtcgctaaac ggattgcgcc ggcttccgga
      181 agcgtccccc gaacttccca cctatctcgg acttcaggcg tgctctgcaa tcgctctgat
      241 tgtggttctc ctccggtccg aattccgaat cttccacgag gcgattgtca gggcgttcac
      301 aatccagctt ctttggcgag gaaagtgatc catgggcata ccgcgcaaaa gattagccga
      361 acccgagcgc aggacacgga ttgtcagctt tcggctttcc ggtgatgagt acacgaggct
      421 ccagcagatc tcggagagcc atggctcacg aagtatttcc gagttcttcc gccagctcat
      481 cgagagaggc gtgccggcag acccgcagaa agcctttgac cgacggctgc gcgaattttg
      541 cgaggaactg gaccgtctca attcggaagt gcagcgaatc gcccaccttg tcgagcgagg
      601 ctgagaacaa atccatggca cgcgacatga cacgatattt catagggtca ctcctcctgg
      661 cggccggctg tctctccgcg gagcagccgg ccgcccagca gccggataac tcaccggcag
      721 ggcaaaatcc aattcactcc tcctacgtcc tgggtccgga tgaccagctt tccatccggg
      781 ccctcgacgc cccggagatc accgacaagc cactgagaat cagtaccagc ggccagatca
      841 gccttccgat ggtggggcgt ttgcgcgccg ccggactgac ggtggacgaa ctcgagagac
      901 agttgtcgga ccggctgcgg gtctacgtac gtgatccgca agtcgcgatc tcggtgactg
      961 agtttcgcag ccagccggtc tcggtgatcg gcgccgtcaa ccagcccggc atccaccagc
     1021 ttcagggaac gaagagactc atcgaggtcc tctcgctggc aggcgggctc cgcaacgacg
     1081 ccggaaacac ggttaccatt acgcggcgca aggagtgggg cgagatacca ctgcccaacg
     1141 tcaagccgga tgccagcggg gagtttacga tcgccgacgt aagcgtcaag tcgatcatgg
     1201 cggccgagaa tccaaaggcc aacatcagca tcctccccaa tgacgtgatt accgtgccga
     1261 aggccgatct ggtctacgtg atcggatcgg tgcgccgctc cggcggcttc gtgctcggcg
     1321 aacgggagac gatctcggtg ttgcaggcgc tgtcgctcgc cgagggactc gatcgcacgt
     1381 ccgctcctcg aaacgccaag attctccggc tgtccggcaa cgacaacaag gaccgctccg
     1441 agatcgccgt cgacgtcaag aagatcctcg agggaaagac aggcgacgtc tggatgcagg
     1501 cgaacgacgt gctgttcatc cccggaagcg cggcgaaatc ggcgaccttg cgcggcattg
     1561 aggccgccat ccaggttggg accggagtcg caatctggcg gcgataagga tcacggcgcg
     1621 tctcaactcc aaccgacaaa cgagagcgag caaagatgaa acaaatccct tcgctacccc
     1681 ccggccgtga gcccgtcgaa atcacgccga gcaacggtca tggccagcca tccaagttcg
     1741 gctacatcga ggcgcaagga tacacctacg cccaaggtta tcccgccgaa gaggctgatg
     1801 cgcccggtct tgtcgagtac tcgcggattc tccgccgacg caagggcacg ctcatcctcg
     1861 tctcgttcct cgggctgtcg gccgcggtgc tgatcaccgt gccgcagacc ccgatctttc
     1921 agtcgcgcac ggccatcgag atccaggaca tcaacgacga ctttcttaac atcaagcagg
     1981 tcacgccggt gcaggacgcg ccatcaacgg gccacctcac ggacattcag acccaggtca
     2041 agatcatcca gagcgaaacg ctgcgcgaga gatcggcgaa ccttctcaag aagaagcagc
     2101 cggcgagctt caacgcccgg aggggccggt tcgccgcgtg gagggaactg ctccgcctgc
     2161 cgaagtcgag cccgatgacg ccttacgagc tcgaggtgga aaacatcagg aaagaggttc
     2221 aggtaaagcc ctccggacag acaaggattg tcgaggttct gtgcgactcc accgaccgga
     2281 atctggcggc ggccttctgc aacacgatgg taagcgagta catcgaccag aacatggagg
     2341 cgcgctggac gatgagcgag aggaccggcg agtggctttc gcgccagctc gcggagatga
     2401 gggtcaggct ggagcgctcc gaggacggct tacagaatta cgcccgccag tcgggcctgt
     2461 tgttcacctc cgagaaagag aacgtatccg acgagaaact tcgccagttg caggcggcgc
     2521 tctcggccgc gcaggccgac cgggtggcca agcagtcgag gtacgagatc atgcggacgg
     2581 tctccgccga cacgctgtcg gacgccgtgc cggacggctc gctcaacttc ctggtgacga
     2641 ggctcgccga cttgaggcgc caggaagcgg agctgacgac caccttcacg acggaacaca
     2701 gcagcgtgaa aagggttcgc gctcagatcg ccagcctcga gaagactctc aacgaacagc
     2761 gcggcatcat gctgaatcgc atccggaacg actatgagga ggcgttgaag cgggagaagc
     2821 tgctcggggc cgcctacgcg agccagtcgc gcctggtcac gcaacaggcc gagaagtcga
     2881 ttcagtacaa catcctcaag cgcgaggtgg attcgaaccg gcagctctac gagtccatgc
     2941 tccagcgagt gaaggaatcg agcatcgtct cggcgatgcg cgcgagcaac gtccgggtgg
     3001 tggacatggc caggccggcc aaatccccgt acaagccgcg actggcactg aactcgctgc
     3061 ttggcctct
//
LOCUS       DATIFQ010000218        14986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_455623, whole genome shotgun sequence.
ACCESSION   DATIFQ010000218 DATIFQ010000000
VERSION     DATIFQ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455623"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VLE22_10295"
     CDS             complement(<1..356)
                     /locus_tag="VLE22_10295"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB14841.1"
                     /translation="MSARERELLAVPYVHLVFTVPHALAPLILHNKKLLYALLFRASA
                     ETLLEIAADPEHLGAHIGFFSVLHTWGQNLMHHPHVHCVIPSGGLSPDHKAWVHPRYR
                     FFLPRGVLRKVFRGKFV"
     gene            complement(602..1492)
                     /locus_tag="VLE22_10300"
     CDS             complement(602..1492)
                     /locus_tag="VLE22_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HSB14842.1"
                     /translation="MTHLRKLMLDELQRRNYAQSTAEAYVHALRKLAQHFHLPPDRLG
                     PEQIRDHQLYLMREKGLSPSTVKQHMAAMKFFFVHTLKRSFRWDQLPYPKVPKRLPII
                     LSKEEVRWMINAASDLFHRAILMTLYSTGMRRDELVRLKVSSIDSGRMMIRIQRGKGG
                     KDRDVPLSPKLLETLREYWRWMKPNSYLFPSRSSRRKGLPISGRGVFEICRSTAKRAG
                     IQKRLGPHTLRHSYATHLLDDGADLRTIQVLLGHSSLNHTTIYLHLSQRHLQAIPNPL
                     DGLGVCDVVEVRRSRETPKQ"
     gene            1872..2141
                     /locus_tag="VLE22_10305"
     CDS             1872..2141
                     /locus_tag="VLE22_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HSB14843.1"
                     /translation="MEAEWDPVKAKANLRKHNVRFADAVTVLEDARAITVRDEGAGEE
                     RWVTIGVDSLGRILVVVYTWRDERVRLISARPATRREARQYEQEV"
     gene            2125..2379
                     /locus_tag="VLE22_10310"
     CDS             2125..2379
                     /locus_tag="VLE22_10310"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HSB14844.1"
                     /translation="MSKKYDFSKGRRGPVLKPPPGKTRVTIRLDDDVLEWFRQQVDDA
                     GGGNYQTLINEALHSFMHQKQESLEMTLRRVIRDEIRRAS"
     gene            2616..3365
                     /locus_tag="VLE22_10315"
     CDS             2616..3365
                     /locus_tag="VLE22_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB14845.1"
                     /translation="MSSTTTWPTFMAKLMERQEVANRTLALRFEKPADSPFTPGQFVE
                     VTLMNPAETDAEGNSRVFSIASAPHESFLMVTTRLRDTAFKRTLRTMPLSAEVRIEGP
                     FGDLRLHKNQARPAVFLAGGIGITPIRSIVLHAAKQKLPHRIFLFYSNRRPEDAAFLQ
                     ELDALQQENRNYRFIPTMTEMEKSQAHWQGETGHFTPGMLTKYLKPASPDATPIYYLT
                     GPPGMVSGVRAMLIGAGIDEDDIRTEEFSGY"
     gene            3408..4757
                     /locus_tag="VLE22_10320"
     CDS             3408..4757
                     /locus_tag="VLE22_10320"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HSB14846.1"
                     /translation="MNEDSRIEHDTMGEMRVPRDALYGAQTARAVENFPISRMRFQRP
                     FLRALGLIKAAAAHVNGRMGHLPPAKAAAIEAAANEVAEGRHDDAFVVDVFQSGSGTS
                     TNMNANEVIGRLSNAHPNDEVNRGQSSNDVIPAALHIAAVEMVRGQLRPAMRGLQGAL
                     ESKAREFDDVIKIGRTHLQDAVPLRLGQEFLGYARQVEAALERVEAALTGMYELPLGG
                     TAVGTGINAPEGFGTSVIAELAARTGLFFREAANHFEAQGARDAAVFLSAALRNYALA
                     LIKIANDIRWLGSGPRCGIGELQLPATQPGSSIMPGKVNPVIAESLLMVCAQVIGHDA
                     AIAWCGAAGNFELNAMMPIMAWDLLDSIELLASGTRNFETRLVRGLEANRERAEAFVE
                     QSLALATALVPIIGYEQAAALAEEARASGRTIREVARERSGIPSDELERLLAPRSQV"
     gene            complement(4792..6075)
                     /locus_tag="VLE22_10325"
     CDS             complement(4792..6075)
                     /locus_tag="VLE22_10325"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSB14847.1"
                     /translation="MSGEQRHRVVIVGGGFGGLQAAKALRNAPVEVTLIDRRNFHLFQ
                     PLLYQVATSGLSPGDIAAPLRRILRRQRNTRVIMDEVTGVDVGRRMVILKNREVPYDT
                     LVVAAGASHSYFGNQQWEEVAPGLKTLEQATEIRRRVLLAFEAAEIATDQAARRALLT
                     FVIVGAGPTGVELAGTLGEVANDTLRGDFRTIDPAEAQIIVVDRSPRVLPGFPEDLSA
                     AAERSLIKLGVRARTGVGVTHIDREGVTLETPNGSERIAARTVIWAAGVEASPLGRAI
                     AEQTGATADRAGRVSVAPDCSVPGHPEIFIIGDLANYTHQNGKSLPGLCPVAMQQGRY
                     VGHLIRARLGGSACGPFHYIDKGNLATIGRKQAVGILAGLHFSGVVAWLLWLFVHLMY
                     LIGFQNRLLVATQWAFHYFTYNRGARLITGGKKPD"
     gene            complement(6107..6868)
                     /locus_tag="VLE22_10330"
     CDS             complement(6107..6868)
                     /locus_tag="VLE22_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSB14848.1"
                     /translation="MAEQFPHYIEFRNVYKTFDNPVLDDVSFHIDAGETVAIIGRSGV
                     GKSVTLAHIMGFLKPDSGRVIVAHEDITEYLEAELRRVRRKVTMVFQSGALFDSLTVA
                     ENILFSLELREDYNEANKEDVVNGLLGMVDLLEYRDAYPTDLSTGYKRAVAIARALAA
                     QPQCILYDEPTTMVDPIMSDILTSLMLRLKRQLKLTSVVVTHDLDLMRRVADRVVVLH
                     ESRVIYFGPVNELERSNHEHIQEFLAMDKVTLGGG"
     gene            complement(6861..7454)
                     /locus_tag="VLE22_10335"
     CDS             complement(6861..7454)
                     /locus_tag="VLE22_10335"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HSB14849.1"
                     /translation="MNRAGFVLTGGRSSRMGRDKPLLPFRGKTLVEHIGAEVREAAGS
                     VTLVGGAERFRSLNLPAIADIKPDCGPLGGIVTALEFSTAEWNLVVACDMPAVSASFL
                     RSLLDDAERTGCECLVPVSPAGRIEPLCAVYRRTCLPELRRVLDTGTRKMTDVLAALN
                     AVKRSVQKSGFFKNLNTPEEWVTHEHSSGSESQGVHG"
     gene            7545..8210
                     /locus_tag="VLE22_10340"
     CDS             7545..8210
                     /locus_tag="VLE22_10340"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSB14850.1"
                     /translation="MIILGVAVVGADSAPAVTAERQNGRVSSVVRIDARTGRLVRIIV
                     APPAVRENSGKPPAEVHEIIVEKAKEHDLDPLLIHSMIQVESNYNAYATSPKGAQGIM
                     QLIPATARRFGVSNSYDVRDNISGGVRYLRFLMDLFQNEKLAVAAYNAGEEAVIRYGG
                     VPPYRETQNYVRLVGDRYREAKRTEAPEVAKADSSEQPDAVTYRPIEEFVDAQGRLHI
                     RTR"
     gene            8217..9635
                     /locus_tag="VLE22_10345"
     CDS             8217..9635
                     /locus_tag="VLE22_10345"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSB14851.1"
                     /translation="MTLAPSRGLIRTLELAGTFLFCLAAPLALHAQQPPALEVKDVRF
                     WSLGEVTRVAVETSGDFEYRYDRLRNPERIFFDVQGAHWRTGARIHEISVGDSLLTRI
                     RVAETQPNITRIVLDLSAPAEFSAAQLANPDRLMIELRPAAPKLPVETVAPQTTPPAK
                     TEPLPVKPPVQAGPVPKAQPAADPAPPAKTSAPAKTEPVLQPQVVEIAKPAEDKVARP
                     AKRTNAGNRTLVRALGLKLGRVVIDPGHGGHDTGSIGRGGLLEKDLVLDVALRLGKLI
                     EEELGAEVVYTRSDDTFVALEDRTALANEKKADLFLSVHANSGNRSAAGPETYYLNFS
                     ASPGDLDVASRENASSEKTIHELQSLIEKIALKEKVDESREFAADVQSALHLALGKTR
                     SLKDRGVRKAPFVVLIGAQMPSVLAEIAFLSNPREERLLRRADYRQKVAQGLYKGVAE
                     YASSLSHFQVAEKKSETSGKVR"
     gene            9650..10336
                     /locus_tag="VLE22_10350"
     CDS             9650..10336
                     /locus_tag="VLE22_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSB14852.1"
                     /translation="MRVLAFRHVPFEHLGLIVPALGAHGVAFEYVDLFRDASIPLDLD
                     SAAGLIFMGGPMSVNDNLPFLEAELRLIERAVAAGKPVLGVCLGAQLIARALGARVYR
                     NEAKEIGWFPVHFTKECAGDSLFDGFNGPETVFHWHGETFDLPEGARLLASSDACRNQ
                     AFRWGRNVYGIQFHLEVTPEMIGDWLTEDENCSDVRELTVRPDPFYNGARLEELSRAV
                     FGRWAGLCKT"
     gene            10421..10888
                     /locus_tag="VLE22_10355"
     CDS             10421..10888
                     /locus_tag="VLE22_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSB14853.1"
                     /translation="MIYSRSAEYAIRAFVHLAQVPDGRFAMVKQIAEREEIPSHFLAK
                     ILQQLARKGLLRSSKGPTGGFSLRQAPGDISLLNIVEALDGLADYQKCASGLAECNDE
                     QACGMHDSWKALRSRIMEYLEHTSIADVASALDEKRRQLEKAKKGKRSAAKKK"
     gene            10927..11544
                     /locus_tag="VLE22_10360"
     CDS             10927..11544
                     /locus_tag="VLE22_10360"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HSB14854.1"
                     /translation="MQSSVLVPMVVEQTSRGERAYDIYSRLLKENIIFLGTPIEDTVA
                     NLVIAQMLFLAAEDPEKDISLYINSPGGSITAGLAILDTMRFVEPDIVTICVGQAASM
                     AAVLLTCGTKGKRYSLPHSRILIHQPSMSGLAGQAADIDIYAKEILRMRENLNQMLAD
                     ATGQSIEKVARDVDRDYIMDPQQAVDYGMIDRVISSRELAPVAQL"
     gene            complement(11576..13051)
                     /locus_tag="VLE22_10365"
     CDS             complement(11576..13051)
                     /locus_tag="VLE22_10365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HSB14855.1"
                     /translation="MKTHYLAFDLGAESGRAILGSFEDSKLALEELHRFPNTPVRTPD
                     ALYWDTFRLFHEIQQGLALAGRERKVEVQGIGVDTWGVDYGLLGRDGSLVANPVHYRD
                     SRTDGILDKMFAAVPKYEIFAHTGIQFMQLNTLCQLYAMKLAGSPGLAAAETLLFMPD
                     LFNYWLTGVKRAELTIASTSQFYDPAGMRWASELLERFGLPASILPEIVRPGAMLGPL
                     LPHLADISGLGPVPVYATACHDTASAVAAVPAEGEDWCYISSGTWSLMGAELDAPVIN
                     ERSLAMNLTNEIGAASKIRLLKNIAGLWLLQECRRAWALEGTEYSYDELTRLAASSRP
                     FGPILNPDVFLHPGQMPAKIAEYCRGTGQPSPEGPVEICRTILESLALRYRQVLESIE
                     SLLGRELSVIHIVGGGSRNRVLNQLVADATGRTVVAGPSEATAAGNILVQAIGSGMVS
                     GLDEARAVVRRSFPPTVVEPKSQAGWDAAYERFSKLTVPAR"
     gene            complement(13048..14082)
                     /locus_tag="VLE22_10370"
     CDS             complement(13048..14082)
                     /locus_tag="VLE22_10370"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSB14856.1"
                     /translation="MKSVQLVAPRTLEVREVPSPPEPGPSEVLVRPKAVGVCGSDLHW
                     YREGRIGWTPAAYPQVLGHEPVAEVAAAGKGAGGFHPGQRVTIEPAIVCGRCEFCLDG
                     QPNNCVRSRFMGGPDQPGLFRELAAVPAGNVVPIPDSLSLVQAALIEPLAVILHLMEL
                     TPIRVGDTVAVLGCGSIGMLCASVARLRGASWVIAADKVEHRLPLAKRMGADVTVLMP
                     RESLRDAVMDHTRGRGVDVVLDAAGATETINAGIGIARRAGVFTLVGLPDEMQLDVDL
                     HTAMAKELRLQMMRRSCHNSHRAIDLLAAGRIPDALVTHRPGLEQTPAAFAMLDEYAG
                     GVGKVVVEIP"
     gene            complement(14612..>14986)
                     /locus_tag="VLE22_10375"
     CDS             complement(14612..>14986)
                     /locus_tag="VLE22_10375"
                     /inference="COORDINATES: protein motif:HMM:NF028067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSB14857.1"
                     /translation="GGNFLLNVGPTPEGTIQPEFEERLLAIGEWLKVNGEAIYGTTYG
                     PLQNLTFGRTTVKGNTIYVHVFDWPAGVLELNGLRAKVSAVRLLAGNRKLTFRQSGDK
                     VTIDIPAQSPSPHAGVVAIETR"
BASE COUNT         2888 a         4631 c         4770 g         2697 t
ORIGIN      
        1 acgaacttgc cacggaacac ttttctaagc acaccacggg ggagaaagaa acgatacctc
       61 ggatggaccc aggctttgtg atcgggggat aagccgccgg acggaatcac gcagtggaca
      121 tgaggatgat gcatcaggtt ctggccccag gtatggagca cgctgaagaa acctatgtgc
      181 gcacccagat gctccggatc ggcggcgatc tccagaagag tttctgcgct ggctcggaac
      241 aggagcgcgt agagaagctt cttgttgtgc aggatcagag gagccagcgc atggggaacg
      301 gtgaaaacca gatgcacgta gggaacggca aggagttccc tttcacgggc ggacaaccac
      361 ttcttgcggg cgttgttccg acaccgcgga caatgccggt tgcggcagct attgtacgag
      421 atggcaacga catgaccgca tcgggaacaa cgatccttgt ggcctccgag tgctgccgtg
      481 cggcaacgtc ggatggcgga aagcacctta cggtgcagcc ccgtcagcca ggaccggttc
      541 ctgtcgatga aatccttgcc tgccgagcgg attatatcgg ccacctcgat gggcggcctg
      601 ttcactgctt gggcgtttcc ctcgagcgac gcacctcgac cacatcacag acgcccaagc
      661 catccagcgg gttcggaatt gcctgcagat ggcgctgcga taaatgcaga tagatcgtcg
      721 tgtgattcag gctgctgtgc ccgagcagaa cctggattgt gcgcaggtct gctccgtcgt
      781 ccagaagatg ggtggcatag gagtgccgca gcgtatgcgg accaagcctc ttctggatac
      841 cggcccgctt cgcggtcgat cgacagatct cgaacacgcc cctgcccgaa atcggcagac
      901 cctttcgacg gctggatcgg cttggaaaca ggtacgagtt cggtttcatc cagcgccaat
      961 actcccgcag cgtctcgagc agcttcgggc tgaggggcac gtcgcggtcc ttcccaccct
     1021 tgccacgctg gatacgaatc atcatgcgcc cgctgtcgat actgctgacc ttgagccgga
     1081 ccagctcatc acggcgcata cctgtggagt agagcgtcat caggatggcg cgatgaaaga
     1141 ggtcgcttgc cgcattgatc atccatctca cctcttcctt gctgaggatg atcggcaatc
     1201 gcttcggaac tttagggtac ggcagctgat cccagcgaaa ggatctcttg agggtgtgga
     1261 caaagaagaa cttcatcgcg gccatgtgct gcttgaccgt gctgggcgaa agccccttct
     1321 ctcgcatcaa gtaaagctga tggtcacgta tctgttcagg gcctaatcga tctggaggta
     1381 aatggaagtg ctgcgccaac tttcgtagtg cgtgcacgta ggcttctgca gtactctgag
     1441 cgtaattgcg acgctggagt tcgtccagca tcaactttcg tagatgggtc acatggacct
     1501 cctttctatg tgacccacgc tatctcaacg gcagtaaatc ggaatacagc acacgcgcag
     1561 cgtccgccgc gccagcggct tcgttcaagt cgcctattcg tagaactggt ttgaggcgac
     1621 acactccggg agtagtggtt agccagaggt acgtgccgtg atgtatcagt gaggacgcgg
     1681 cacgggaata cgggacggtt cgttaagcct cgatccagtc gagcgaatgc gcgggctgca
     1741 ttggaggctg agcgcgggcg tggtgatcgg agtgttccat tcgtgcggag tgggccccag
     1801 gcgcccaagt gggggaccag ggaaattagt ctctggcgaa cccgttgaag cggtgtacaa
     1861 tagtacacaa gatggaggcc gaatgggatc ccgtcaaggc aaaagcgaat ctccgcaaac
     1921 acaatgtccg attcgccgac gcggtgaccg tgttggaaga tgcgagggcg ataaccgttc
     1981 gcgacgaagg ggcgggcgaa gagcgctggg ttaccattgg cgtggattcc ctgggccgca
     2041 ttcttgtggt cgtctacact tggcgcgatg agcgggttcg gctgatttca gcccgccccg
     2101 cgactcgcag agaggcgcgg caatatgagc aagaagtatg atttcagcaa aggccggcgc
     2161 ggaccagttt taaagccgcc accgggaaag acccgggtca cgatccggct ggacgatgac
     2221 gttctggagt ggtttcgtca gcaggttgat gacgcaggag gcgggaacta ccagacgctc
     2281 atcaacgagg cgttgcacag tttcatgcac cagaagcagg agtccctgga gatgaccctg
     2341 cggcgagtga ttcgagacga aatccgccgc gccagctagc gctggcgagc atcgatgctg
     2401 ccggcgttct tggatgggag tgatgcgtcg aatggttcgg cttcctccca aagggtaggt
     2461 gttcatcctc gaaacggagc atggctgcct cgacgacgtt cgagcgcaac tcagttccac
     2521 gcccatagaa tacctgaggc cgaacggacg gtggtacgtt gaaagcagaa gggtttgagc
     2581 ctgatgtcca aatctgggat ccaaacgagg tcagcatgag ttccacgacg acttggccga
     2641 cattcatggc aaagctcatg gaacgccagg aggtggccaa ccgcactctg gcgctccgat
     2701 tcgaaaaacc ggcggacagc ccctttacgc ccggtcagtt cgtagaggtc actctgatga
     2761 accccgcgga aactgacgcg gaaggaaact caagggtatt ttccatcgcc agcgctccac
     2821 atgagtcgtt cctgatggtc acgacgagac tgcgggacac ggctttcaag aggacgctga
     2881 ggaccatgcc tttgtcagcc gaagttcgga tcgagggccc cttcggcgac ttgcgcctgc
     2941 acaagaacca ggcgcggccg gccgtgttcc tggcgggtgg aatcggaatc acgcctatcc
     3001 gcagtatcgt gcttcatgcg gcgaagcaga aactgccgca ccgcattttc cttttctatt
     3061 ccaaccgacg ccctgaagat gccgccttcc tgcaagagct tgatgcccta cagcaggaga
     3121 accgcaacta ccggttcatc cccaccatga cggagatgga aaagtcgcag gctcactggc
     3181 agggagaaac gggccatttc accccgggca tgctcacaaa gtatctgaag cccgcgtccc
     3241 ccgacgcgac cccgatttac tacctcactg gcccgcctgg aatggttagt ggagtacgtg
     3301 cgatgctcat cggcgccgga atcgacgagg acgacattcg cacggaagaa ttcagcgggt
     3361 attgatcggg cctttctcgg ttgacctccg cgcccgaggg caggcctatg aacgaagact
     3421 cccggatcga acacgacacc atgggtgaaa tgcgcgtgcc gcgggatgca ctctacggag
     3481 cgcagaccgc ccgggccgtg gaaaacttcc ccatcagccg gatgcgtttt cagcggccgt
     3541 tccttcgggc gctcgggctc atcaaggccg ccgccgccca tgtgaacggt cgaatgggtc
     3601 atctgccgcc ggcgaaggcg gctgcgatcg aagctgccgc caatgaagtt gcggagggac
     3661 gccacgacga cgcgttcgtt gtggacgtct ttcagagcgg cagcggcacc tcgaccaaca
     3721 tgaacgccaa cgaggtgatt ggccgcctgt cgaacgcgca tccgaacgac gaggtcaacc
     3781 gcgggcaatc cagcaatgac gtaattccgg cggccttgca cattgcggcc gtcgaaatgg
     3841 ttcgcggcca actgcggccc gcgatgcgcg gcctgcaagg cgcactggaa tcgaaagccc
     3901 gggaattcga cgacgtgatc aaaatcggcc ggacacacct tcaggacgcc gttcccctga
     3961 gactgggaca ggagttcttg ggatacgcac ggcaggtcga agcggcgctc gagcgcgtgg
     4021 aggcggccct aaccggcatg tacgaactgc ccctgggagg cactgcggtg ggtacgggga
     4081 tcaacgcccc cgaggggttt ggaacttcgg tcatcgccga gctggccgcc cgcaccggcc
     4141 ttttctttcg cgaagcggcc aaccatttcg aagcgcaggg cgcgcgcgac gccgcggtct
     4201 ttctctccgc cgcgctgcgg aactacgcgc tggccctcat caagattgcc aacgacatcc
     4261 gctggctcgg ctcgggaccg cgctgtggca tcggcgaact tcaactgccc gccacgcagc
     4321 ccggctcgag catcatgccc gggaaggtca atccggtgat cgccgagagc ctgttgatgg
     4381 tgtgcgcgca agtgatcggc cacgatgccg ctattgcctg gtgcggcgcg gcaggcaact
     4441 tcgaattaaa cgccatgatg ccaatcatgg cctgggatct gttggattcg atcgagctgc
     4501 tggcttcagg cacccgaaat ttcgaaacgc gcctggtgcg cgggctggaa gccaaccggg
     4561 aacgcgccga ggcgttcgtc gagcagtcct tggccctggc caccgcgctg gttccgatca
     4621 tcggctatga gcaagccgcg gcactggccg aggaagcgcg cgccagcggg cgcacgatcc
     4681 gggaggtggc ccgcgagcgc agtggcatcc cttccgacga actggagcgt ttgctcgctc
     4741 cccgcagcca ggtctgacgc tcgggagtgt gagcgccctg gcaacgcgcc tctagtcagg
     4801 cttcttcccg ccggtgatca ggcgggcacc gcggttgtaa gtgaagtagt ggaacgccca
     4861 ttgtgtcgcc accagcaggc ggttctggaa gccgatcagg tacatcaggt ggacgaaaag
     4921 ccacagcagc caagccacca cgccgctgaa gtgcagtccc gccagtatcc ccaccgcttg
     4981 cttccgcccg atggtggcca ggttgccctt gtcgatatag tggaaaggcc cgcacgcact
     5041 accgccgagc cgtgcgcgga tcagatgtcc gacataccgc ccctgctgca tcgccaccgg
     5101 gcagagcccg ggtaggctct ttccgttttg atgggtgtag ttcgccagat cgccgatgat
     5161 gaagatctcg ggatggccgg gaacgctgca atcaggcgcg acggagactc ggccggcgcg
     5221 gtcggccgtc gcccccgtct gttccgcgat cgcgcgcccg agcgggctcg cttcgacgcc
     5281 ggctgcccag atcaccgtcc gcgcggcgat ccgctcggag ccgttcggcg tctccagagt
     5341 gaccccttcc cgatcgatgt gcgtcacacc cacgcccgtt cgcgcacgga cgccaagctt
     5401 gatcaacgac ctttcggcgg ccgccgacag gtcttccgga aaaccgggca gaaccctcgg
     5461 cgagcgatcc acgacgatga tctgcgcttc ggcgggatcg atggtgcgga aatcgcctcg
     5521 cagggtatcg tttgccacct caccgagggt cccggcgagt tccacgcccg ttgggcccgc
     5581 accgacgatg acaaaggtca gcaaggcgcg ccgggcagcc tgatccgtgg ctatttcggc
     5641 ggcttcgaaa gccagaagca cccggcggcg aatctccgtc gcttgctcca aagtcttcag
     5701 gcccggcgcg acctcttccc actgctggtt gccgaagtag ctgtggctgg ctcctgccgc
     5761 gaccaccagc gtgtcgtacg gcacttcccg attcttcaga atcaccatac ggcgtccgac
     5821 gtccacgccg gtgacctcat ccatgatcac gcgggtgttc cgctgccgcc gcaggatacg
     5881 gcgtagcggg gctgcaatgt caccgggaga gagcccgctt gtcgccacct ggtagagcag
     5941 aggctggaag aggtggaagt tgcggcggtc gatgagtgtg acttcgaccg gcgcgttcct
     6001 taacgccttg gcggcctgca accctccaaa gccgccgccg acgatgacta ccctgtgccg
     6061 ttgttcccca gacatgccgt tggctgaccg gtgaccggcg agacgctcac ccgcctccca
     6121 gtgtcacctt gtccatcgcc agaaactcct gaatgtgctc gtggttggac cgctccagct
     6181 cgtttacggg accgaagtaa atcacgcggc tctcgtgaag gaccacgacg cggtccgcca
     6241 cccggcgcat caggtcgaga tcgtgcgtca ccaccaccga cgtcagcttg agctggcgtt
     6301 tcaggcgcaa catcaggctg gtgaggatgt ccgacatgat cggatccacc atggtggtcg
     6361 gctcatcgta caagatgcac tgcggctgag cggcaagcgc cctcgcgatc gcgacggccc
     6421 gcttgtagcc ggtcgaaaga tcggtcggat acgcatcccg gtactccagc agatccacca
     6481 tgccgagcag gccgttcacg acatcttcct tgttggcttc gttgtagtcc tcacgcagct
     6541 caagcgagaa gaggatgttc tcggccacgg tcagggagtc gaacaaagcc ccggactgga
     6601 aaaccatggt gactttccgg cgcacccgcc gcagttccgc ctccaaatat tcggtgatat
     6661 cttcgtgcgc cacgatcacc ctgccactgt ccggtttcag gaaacccatt atgtgagcta
     6721 gggttacgga ctttccgacg ccgctacggc cgatgatcgc gacggtctcc cccgcgtcga
     6781 tatggaaact cacgtcgtcc aaaacggggt tgtcgaacgt cttatagacg ttgcggaact
     6841 cgatgtagtg cggaaactgc tcagccatgg actccttgag actcgctccc ggaggagtgt
     6901 tcgtgcgtga cccactcctc cggggtgttg agattcttga agaatccgga cttctgcacg
     6961 gagcgtttca cggcattcag agccgccagg acgtcggtca ttttccgggt tcccgtgtcg
     7021 agaacgcgcc gcaattccgg caggcacgtc cggcgataga ccgcgcacaa aggctcgatg
     7081 cggcctgctg gcgacaccgg cacgaggcat tcgcaacccg tacgttcggc gtcatcgagc
     7141 agggaccgga gaaaactcgc ggaaacggcc ggcatgtcgc aagccacgac gaggttccat
     7201 tcagccgtcg agaactccaa cgccgtgacg attcctccga gcggtccgca gtccggcttg
     7261 atatccgcaa tcgcgggcag attgagcgag cgaaatcgct ccgctccccc gaccagtgtg
     7321 accgagcccg ccgcctcgcg aacttcggcg ccgatgtgct ccaccagcgt ctttccgcgg
     7381 aacggaagca acggcttgtc ccgccccatc cgggaacttc ggcccccggt aaggacgaag
     7441 ccggccctgt tcatgcaaaa atgataacat ccggtccggt gaaaaaccga ttcgctggga
     7501 cggagattaa ggctataatt tagaacaaag tcatgcggtt ttgtttgata attctgggag
     7561 tggcagttgt cggggcagac agcgctccgg ccgtgacggc ggagcgtcaa aacgggcgag
     7621 tttcgtccgt tgtgcggatc gacgccagaa cgggccggct ggttcgaatc atagtcgccc
     7681 cgccggcggt gcgggagaac tccggcaagc cgcccgccga ggtgcacgag attatcgtgg
     7741 agaaggcaaa ggagcacgac ctggacccgc tgctgatcca ctcgatgatc caagtggaga
     7801 gcaactataa cgcctacgcc acttcgccca agggagcgca gggcatcatg caactgatcc
     7861 cggcgacggc ccgccggttc ggcgtgagca acagttacga cgttcgggac aacatttccg
     7921 gcggcgtccg ctacctgcgg ttcttgatgg accttttcca gaacgagaag ctcgccgtgg
     7981 ctgcgtacaa cgctggcgag gaggcggtga tccgctacgg tggggtgccg ccgtatcgcg
     8041 agacgcagaa ctacgttcgc ctggtcggcg accggtaccg cgaggccaaa cggaccgagg
     8101 cgcccgaagt cgcgaaggcg gattcttcgg aacagcccga cgccgtaacg taccggccga
     8161 tcgaggaatt cgtggatgcg caggggaggc tgcacatccg tacccgttag ccgctcatga
     8221 cccttgcccc cagccgcggt ttgatccgga ccctggaact ggcgggcact ttcctctttt
     8281 gcctggctgc gcccctcgcg ctccacgcgc agcaaccgcc cgcgctcgaa gtcaaggatg
     8341 ttcgcttctg gtcactcggg gaggtgaccc gtgtcgccgt ggagacgagc ggggacttcg
     8401 aataccgcta cgaccggttg agaaaccccg agcgcatctt cttcgacgta cagggcgcgc
     8461 actggcgcac gggtgctcgc attcacgaga tctccgtggg cgactcgttg ctgacgcgca
     8521 ttcgggtagc cgagacgcag ccgaatatca cgcgaatcgt gctggatctg tccgcgccgg
     8581 ccgagttcag cgctgcccaa cttgccaacc ccgaccgcct catgatcgag ctgaggccgg
     8641 ccgcgcccaa gctcccggtg gaaaccgtgg ctccgcaaac gacgccgccg gccaaaaccg
     8701 agccgctgcc cgtaaagccg ccggttcaag ctgggccggt gccgaaggcg caaccggccg
     8761 cggatcccgc tccgccggcc aaaacgtcgg cgcctgccaa aacggagccg gtcctccaac
     8821 cccaggtagt cgagattgcc aagccggccg aggacaaagt cgctcggccg gccaagcgga
     8881 ctaacgccgg aaaccgcacc ttggttcgcg ccctcggtct caaactgggg cgcgtcgtga
     8941 tcgatcccgg acatggcggc cacgataccg ggtcgatcgg ccgtggcggg cttctcgaaa
     9001 aagacctcgt gctcgatgtg gccttgcgcc tcggcaagct catcgaggaa gaactcggcg
     9061 cggaagtcgt ctatacgcgt tcggacgaca ctttcgtagc gctcgaggat cgcacggcgc
     9121 tggcgaatga aaagaaagcc gatctgtttc tctccgtcca cgcgaattcc ggcaaccggt
     9181 cggcggccgg cccggagacc tactatctga acttctccgc gtcgccgggc gacctggacg
     9241 tcgcctcgcg ggagaacgcc agctccgaaa aaacaatcca cgagttgcag agtctgatcg
     9301 agaagattgc cttgaaggaa aaggtggatg aatcgcggga gttcgctgcg gacgttcagt
     9361 ccgcgttgca ccttgcgctg gggaaaacaa ggagcctgaa ggaccgcgga gtgcggaagg
     9421 cgccgttcgt ggtgctgatc ggcgcgcaga tgccgtcggt gctcgcggaa atcgcctttc
     9481 tcagcaaccc gcgcgaggag cggctgttga ggcgtgccga ctaccgccag aaggtcgctc
     9541 agggattgta caaaggcgtg gcggaatacg cgtcgtccct aagccatttt caggtcgcgg
     9601 aaaagaagtc cgaaacaagc ggaaaggtcc gctgaatcga gcctccggta tgcgcgtgtt
     9661 ggcgttccgg cacgtaccgt tcgagcacct cggcctgatc gtcccggcgc tgggggcgca
     9721 cggtgtcgcg ttcgagtacg tcgatctttt tcgtgatgcc tccatcccgc tggacctcga
     9781 ttcggccgcc ggcctgatct tcatgggcgg cccgatgtcg gtgaacgaca atctgccgtt
     9841 ccttgaggcg gagcttcggc tgatcgagcg tgccgtggcg gcaggcaagc ccgtgcttgg
     9901 ggtgtgcctc ggggcgcagt tgatcgcgcg agcgttgggc gcgcgtgtat atcggaacga
     9961 agccaaagag atcggctggt ttccggtcca ctttaccaag gagtgcgccg gtgacagtct
    10021 cttcgacgga ttcaacgggc cggagaccgt ctttcactgg cacggcgaga cgtttgatct
    10081 gccggagggc gcccggctgc tggcgtcgtc ggacgcctgc cgaaatcagg cttttcggtg
    10141 ggggcggaat gtttacggca tccagtttca cctcgaagtg acgccggaaa tgatcgggga
    10201 ttggctcacg gaggatgaga actgttccga tgtgcgggag cttacggtgc ggccggatcc
    10261 gttttacaac ggcgcccgtc ttgaagagct ctcccgggcg gtgttcgggc ggtgggctgg
    10321 gctctgtaaa acgtgaaatt tgcgctgccc tcttgcgcga agtcccataa actcattaag
    10381 atattggagt actgaaatct ccgtatcttg ggggcaaagg atgatttatt cgcgttccgc
    10441 cgagtatgcg attcgggctt ttgtgcacct ggcacaggtg ccagacggga gatttgccat
    10501 ggtcaagcaa atcgccgagc gagaggagat cccgtctcac tttctggcca aaatcctgca
    10561 acaactggcg cgcaagggtc tccttcgttc cagcaaaggt ccgaccggcg gtttcagcct
    10621 tcgccaggcg cctggcgaca tctctctgct gaacatcgtc gaagcgttgg acgggcttgc
    10681 ggactaccag aaatgcgcga gcggcctcgc ggaatgcaat gacgagcagg cgtgcggcat
    10741 gcacgactcc tggaaggctc tgcgttcgcg tatcatggaa tatctggagc atacttcgat
    10801 cgcagacgtg gcgagcgcgc tcgatgagaa gcgccgccag ttggagaaag cgaagaaggg
    10861 caagcgctcc gcggcaaaga agaagtaagc gccctcgccg gatgggggcc gacggaggaa
    10921 caagggatgc agagttccgt gctggttccc atggtggtcg aacagacttc ccggggcgaa
    10981 cgcgcctacg atatttactc gcgccttctc aaagagaaca tcatctttct cggcacgccg
    11041 atcgaggata cggtcgctaa cctcgtgatt gcacagatgc ttttcctggc ggccgaggac
    11101 cccgaaaagg acatctcgct ttacatcaac tcgccgggcg gatcgatcac cgccggactc
    11161 gcgatcctgg acacgatgcg gtttgtcgaa ccggacatcg tgactatctg cgtcgggcag
    11221 gcggcctcaa tggccgccgt gctgctcacc tgtggcacga agggtaagag gtacagcctt
    11281 ccgcactcgc gcatcctcat ccaccagccg tcgatgagcg ggctggcggg gcaggccgcc
    11341 gacattgaca tctacgcgaa ggaaatcctg cggatgcggg agaacctgaa tcagatgctc
    11401 gccgatgcca ccgggcagtc gatcgagaag gtggctcggg acgtggaccg ggactacatc
    11461 atggatccgc agcaggcggt ggactacggc atgatcgacc gcgtgatctc cagccgcgaa
    11521 ttggcgccgg tcgcgcaatt gtaagcccgc accccggggt tctgagacga ttcggtcacc
    11581 gtgccggaac ggtgagtttc gagaaccgct cgtacgcggc gtcccagccc gcctgtgact
    11641 tcggctcgac gacggtgggg gggaaggaac gccggacgac cgcacgcgcc tcatccagtc
    11701 ccgacaccat ccctgagccg atcgcctgaa cgagaatatt tccggcggcg gtcgcctccg
    11761 acggacccgc gacgaccgtc cggccggtgg cgtcggccac cagttgattg agcacccggt
    11821 tgcgggaacc accacccacg atgtggatca ccgacagttc ccggccaagc agcgactcga
    11881 tgctctcgag cacctgccgg tagcgcagcg ccaggctctc cagaatcgtc cggcagatct
    11941 ccaccggtcc ttccggagaa ggctgcccgg ttccgcggca gtattcggca atcttcgcgg
    12001 gcatctggcc ggggtggaga aagacatccg ggttgaggat cgggccgaac ggccggctcg
    12061 aggctgccag ccgcgtgagc tcgtcgtagc tgtattcggt tccttcgagc gcccaggcgc
    12121 gccggcactc ctgcaacaac cacaacccgg cgatattctt gagcagccgg atctttgagg
    12181 cggcgccgat ctcgttggtc agattcatcg cgagcgaccg ctcgttgatc accggcgcgt
    12241 caagctccgc tcccatcaag gaccacgtgc cggagctgat gtagcaccag tcctcgcctt
    12301 ccgcgggcac tgcagccacg gccgacgccg tatcgtggca ggccgtcgcg tacaccggaa
    12361 cggggccgag cccggagatg tccgccaggt gcggcagcag aggtccgagc atcgctcccg
    12421 gacgcacgat ttccggcagg atcgacgccg gcaacccaaa ccgctcaagc agttcggacg
    12481 cccagcgcat tccggccgga tcgtaaaact gcgatgtgct ggcgatagtc agctccgcgc
    12541 gcttcacgcc ggtgagccag taattgaaca ggtccggcat gaacagcagc gtttcggcgg
    12601 cggcgagccc gggcgagccg gccagcttca tggcataaag ctggcacagc gtgttgagct
    12661 gcatgaactg tataccagtg tgggcgaaga tttcgtactt cggcacggcg gcgaacatct
    12721 tgtcgagtat gccgtcggtc cgtgaatcgc ggtagtgcac cgggttggcc accagcgagc
    12781 cgtcgcggcc gagcagtccg tagtccacgc cccaggtgtc cacaccgatt ccctggacct
    12841 cgaccttgcg ctcccgcccg gccagcgcca gtccctgctg gatctcgtga aagagccgga
    12901 aagtgtccca gtagagggcg tccggggtcc ggacgggcgt gttcggaaac cggtgcagtt
    12961 cctccagcgc cagcttggaa tcctcaaagg agccgagaat cgcgcggccg ctttccgcgc
    13021 cgaggtcgaa agcgaggtag tgggtcttca tgggatctcg acaacaactt tgccgacgcc
    13081 gccggcgtat tcgtccaaca tggcgaacgc cgcgggagtc tgctccaggc cgggacggtg
    13141 ggtgaccagc gcgtcgggga ttcggccggc ggcaaggaga tcgatcgccc ggtgtgagtt
    13201 gtggcacgaa cgccgcatca tctggagccg cagttctttc gccatggccg tgtgcaggtc
    13261 gacatcgagc tgcatctcgt ccggcaggcc gaccagcgtg aacacaccgg cgcggcgggc
    13321 gatgccgatg ccggcgttga tcgtctcggt ggcgcccgcg gcgtccagta cgacatccac
    13381 gccccgcccc cgcgtgtgat ccatcacggc gtccctcagg gactcacgcg gcatgagcac
    13441 cgtaacgtct gcccccatcc ttttcgcaag tggcagccgg tgttccacct tgtcggcggc
    13501 aatcacccag gacgcgccgc ggaggcgcgc caccgacgcg cacagcattc cgatggagcc
    13561 gcacccaagt accgccaccg tgtctccgac gcgaatgggc gtcagctcca tcaggtgcag
    13621 aatcaccgcc aggggttcga tgagcgcggc ctggaccagg ctcagggaat ccggaattgg
    13681 gacgacattg ccggcgggaa ccgccgcgag ctcgcgaaac aggccgggct ggtccggtcc
    13741 gcccatgaac cgcgaacgga cgcagttgtt gggctggccg tcgagacaga actcgcagcg
    13801 cccacagacg atcgcgggtt cgatggtgac tctttgccct gggtggaagc cgccggcgcc
    13861 ctttccggcg gcggcaactt cggcgaccgg ctcgtggccg agcacctgtg gataggcggc
    13921 cggggtccaa ccgatgcggc cctcccggta ccagtggagg tcgcttccgc agacgcccac
    13981 cgccttaggg cgaacgagca cttcactagg tcccggttcc gggggcgagg ggacctcgcg
    14041 cacttccagc gtgcgcgggg ccacaagttg aacggatttc atcgcgtaag tgagccagta
    14101 taacgcgagg tcgcggggca cacgaaggct cagaatgcga gggaaggccg tctccggcgg
    14161 aggcgcggaa gcgatattgg cgtggaacgg aggagaggcg ccagggtcca cgcgcagacc
    14221 gcgcttggga cactcacgcc agtcccccgg actatctttc gttcgttcgt catcggcgga
    14281 ccggggtcct accccggccc gccgggcaag gaagtgtcgg cttcacgagg ggaccgatcc
    14341 caccctggcg cgtcaatggc ggcacgttga ccaccaccac aagccctcac gagcgccgcg
    14401 gtcagagtcc acttcgatga aacgtacggt ggccacctgt acgtcgaggc ggtttcctca
    14461 accgcctcct cgatttcagt gtaaggggcc gtgtctacag ttctcgtggg cggccacccg
    14521 taagtggttg agagcaggca gaagaaaaac gagagctgct cgtgattgtc ccgggtctac
    14581 gaacccgagg ggcgctggcc ggcgtattcc tctaccgggt ctcgatggcg acgactccgg
    14641 catgcgggct gggggactgg gccgggatgt cgatggtcac cttatcgccg gactggcgga
    14701 acgtgagctt gcgattgccg gccagcaggc gcaccgctga cactttcgcg cgcaggccgt
    14761 tcagctcgag cacgccggcg ggccagtcaa acacgtgcac atagatcgtg ttgcctttca
    14821 ccgttgtgcg cccgaacgtg aggttctgga gcggtccgta ggtggtgccg tagatcgcct
    14881 caccgttcac cttgagccac tcgccaatgg cgaggaggcg ctcctcgaat tcgggctgaa
    14941 tcgttccctc gggcgtcggg ccgacattga gcaggaagtt gccgcc
//
LOCUS       DATIFQ010000219         2830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1988868, whole genome shotgun sequence.
ACCESSION   DATIFQ010000219 DATIFQ010000000
VERSION     DATIFQ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988868"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..2157)
                     /locus_tag="VLE22_10380"
     CDS             complement(217..2157)
                     /locus_tag="VLE22_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HSB14858.1"
                     /translation="MHENREISSAPWSGDQGRSAKAINRSADAHVLEKSDCAAVPVNQ
                     PNKEGKPSAEAGEGRAQAKENIVQSRMPPTQSGNRMSQGLCGVRTAARERKQERFNSL
                     LHHLSLGLLRDSFYALKRQAVPGVDGMRWQEYETGLEDRLVDLHSRVHRGTYRAQPSR
                     RVYIPKADGRQRPLGVAALEDKIVQQAVVTILNEIYEIDFRGFSYGFRPNRSPHQALD
                     ALTVGIQWKKVNWVLDADIRGFFDNLSHEWAIKFIEHRVADPRIIRLIRKWLKAGVSE
                     DGQWSETKLGTPQGAVVSPLIANVYLHYVFDLWADVWRRKGAKGDMIVVRYADDLVVG
                     FQHRTEAERFLVSFGTGWPSDHAAWPGCYLAAGRNMENVLPLWPGFVLLRMRSTPRCF
                     RTTSCTNESPKPLPPGPFVVKNGSNTLESFSATGPGNAAAGNGPGSGVADIGPGAAPG
                     RGNSPSPGNGGAGKNSTAATAGGVWVGGNAPAGSVSIPSFATSGNAPTPGHSPNQSPR
                     RHPAVTVVGTSRSGGALNHYGALPGSRVYTVYIDTKGGTAVLEYSAVDPSGREFSQDL
                     VAPEAVSAEVPAELRSASFVVSCVMDRSGLLRNVRVLRNSAPLAATQLVAVLQDWRFR
                     PVLLGDAAIEVHAILGFNVDTR"
BASE COUNT          531 a          880 c          821 g          598 t
ORIGIN      
        1 aacagactgc atcgtttgaa tttattccaa gcggatggat tgagagccga acgcttgcgg
       61 tacttagcgc gtgcccgttg gatgttgaac gctggagtgg ggtttcaaat tcactgcggc
      121 ggctggagct gcggatgagc ccgaaataaa aactttggcg ctgtccgcag ctcgcgggca
      181 gcgccgtttt tgtctcacag ccgctggatc gagtgtctaa cgcgtatcta cgttgaaacc
      241 gagaatggcg tgtacctcga tggcggcatc gccgagtagc accgggcgga agcgccaatc
      301 ctgcagcacg gccaccagtt gagtggccgc cagcggcgcg ctgttgcgca gcacgcgcac
      361 attacgcagc aacccggagc ggtccatcac gcaggagacg acaaagctgg cgcttcggag
      421 ttcggcggga acttcggcgc tgaccgcctc aggagcgacc aggtcctgcg agaattcgcg
      481 gcccgacgga tcaacggccg aatattccag caccgccgtg ccgcctttgg tatcgatgta
      541 cacggtgtag acacgcgagc ccggcagtgc gccgtagtgg ttgagggcgc cgccggagcg
      601 cgaggtccca acgacggtca ccgcgggatg acggcgtggc gattggttgg gggaatggcc
      661 gggcgttggc gcgttaccag aggtagcgaa gctgggaatg ctgaccgatc cggcgggagc
      721 gttgccgcca acccatacgc cgccggcggt cgcagcggtg ctgttcttgc cggcgccgcc
      781 gtttccgggg ctgggagaat ttccgcggcc cggcgcggcg cccggtccga tatcggcgac
      841 cccgcttccg ggtccattgc cggcggcagc attgccgggt ccggttgccg aaaagctctc
      901 caaagtattg gaaccgttct ttaccacgaa gggccctgga ggcaatggtt tgggactttc
      961 attggtgcaa gatgttgtgc ggaagcaccg cggggtgctc cgcatccgca gcagcacgaa
     1021 accgggccac agcggcagca cattctccat atttctgcct gcggctaagt aacacccagg
     1081 ccaagccgcg tgatcggagg gccagccggt cccgaaactc acgaggaacc gttcggcctc
     1141 cgtccggtgc tgaaagccca ccacaagatc gtcggcatac cggacgacaa tcatgtcgcc
     1201 tttcgcccct ttcctgcgcc agacatcagc ccacaggtcg aacacgtaat gcagatacac
     1261 gttagcgatc agcggtgaaa ccactgctcc ctgcggcgta cccaacttcg tttccgacca
     1321 ctgaccgtct tccgacactc ccgccttcag ccattttcgg atgaggcgaa ttatccgagg
     1381 atcggcgacc cggtgctcga tgaactttat ggcccattcg tggctcaaat tatcgaagaa
     1441 gccccgaatg tctgcgtcga gcacccagtt caccttcttc cattgaatgc ctaccgtcag
     1501 cgcgtccagc gcctgatgcg ggctacgatt gggacggaac ccgtaagaaa agcccctgaa
     1561 gtcgatctcg taaatctcat tgaggatcgt caccacggcc tgttggacga ttttgtcttc
     1621 cagcgccgcg acgcccaacg ggcgttgccg cccatcagcc ttcggtatat agacccttct
     1681 tgaaggttgc gcccgatacg ttccacggtg aacccggctg tgcagatcga caagtcgatc
     1741 ctcgagtcca gtttcatact cctgccatcg cataccatcg acgcccggca cagcctgacg
     1801 cttgagggcg taaaagctgt cccggaggag gccgaggctg agatggtgga gcaaagagtt
     1861 gaaccgttcc tgcttccttt cccttgctgc tgtccgcaca ccgcacagtc cctgggacat
     1921 gcgattcccg ctctgtgtcg ggggcatgcg cgactgaacg atgttctcct tcgcctgtgc
     1981 ccttccctcc ccagcctccg cagaaggttt cccttccttg ttcggctggt tcaccggtac
     2041 tgcggcacag tccgacttct ccagcacgtg cgcgtccgcc gatcggttta tggccttcgc
     2101 ggaccggcct tgatcaccgg accaaggcgc gctggagatc tcccggttct cgtgcatgtt
     2161 gtttctcagc gtgcgcgggt tcttagacta cgcaggaccg aaacaaccac tcgcgataac
     2221 gtggctgccg tgttgccttc ctccttctcg gaacggagtc ggcatcccga tccatcggct
     2281 tttcgaagct caatagcccg gcccactggt acctctggtc tacgcttcaa gcgacagctc
     2341 gcactgccgc ctgcaagact cgaggccagg atggagtcgc tgttctcctt tcctgtaggg
     2401 ctctttcatc ccctacaaca tgccggttta tcccggcgct ctccggatag ccgactatac
     2461 ttccaattgt ctccacaacg gcggacgaaa ccctttctgt tcagttctga gtgtcaccct
     2521 ctgcttataa ttcgagtgat gctctggacc gtgcctcaag ttgctgaatt cttgcagtgc
     2581 tcaacgtgtt gggtatatcg gcacaagcat gagttgcccc tgatccgtgt cggcggaatg
     2641 ctttggtgtg attcggtccg cctacaggag atggtcagaa gccagccgcc tgttgcgact
     2701 ttaacgcgca cggtcgattc tctgtattgg cacaaaaaat gggggcgcgg ctagagtccc
     2761 cgcattggga agtcgttgaa accttgtggc cgccaagaca acagcctccg tcgcaacgtt
     2821 cgactcacag
//
LOCUS       DATIFQ010000220         4585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1413196, whole genome shotgun sequence.
ACCESSION   DATIFQ010000220 DATIFQ010000000
VERSION     DATIFQ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413196"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VLE22_10385"
     CDS             complement(<1..600)
                     /locus_tag="VLE22_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14859.1"
                     /translation="MTRKNILAFVVFVAIAAPGQRHDAWEVIGPGGGGTMMFPSVSRG
                     NPHDILFASDMSGAYISHDSGSSWRMYNLGGVVRSVIFDPNDARTYYARTSGGYLGKG
                     RALWRSVDAGDTWKLVYPDPAIVTGVFQNDDHASNNIATSASLPGTIEALAVEPGDAH
                     VLYAVMQQGKTSQFFVSQDWGKSWKATANLPGGGRSIYVD"
     gene            745..3558
                     /locus_tag="VLE22_10390"
     CDS             745..3558
                     /locus_tag="VLE22_10390"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14860.1"
                     /translation="MRGRRLTICVAAAFLLLLLNTAYVWAFASPTVFYMSSVLMHVVL
                     GIAVAIGALVLVRLLPTTAAGLLGVSALLGLYLAISALPGLDLVYAGATRANRPILWA
                     HIATAILGVILLIPWISRRAAAQGGGWLRFRKALQICLALLVLLPASTAAYRKLFPNP
                     MDRVRNPMVVPTTMAEEGAGPKSPFWPSSANTNVNGLIPANFFLDSKLCGECHKDIYK
                     QWLGSMHRFSSFNNQFYRKSIEHMQSVIGTKPSRWCAGCHDHAMFFNGRFDRPAIEQI
                     DTPEAQAGLACVSCHSIVHVNSSMGNGGFVIAYPPLHHLASSQNRYIRMLDRFLTYLN
                     PEPHRRTFLKPFMRQNSPEFCSTCHKVHLDVPVNNYRWLRGFNDYDNWQASGVSGQGA
                     RSFYYPAESKTCVDCHMPLVPSKDPGHHDDGKVHSHAFPAANMAVAHANQDQEQMRLT
                     EQFLKSGFITVDIFAASPVDEQPGQSPMWRRSGEGPQLMSTFAVGEEAEQAGQVFLRE
                     VGKLAAPIDQAGARFRPGSTVRVDVVVRTRAIGHFFPGGTVDAFDVWVELRGKDADGR
                     VIFWSGRVEDGGKGPVEKGAHFYRSYMIDGAGNQINKRNAWQARSVLYVRLIPPGAAD
                     TAHFRVAIPKKARGPITLEAKLNYRKFSHYYTQFVYAGQPEPGQPPELVNANHDSRAY
                     FFSPSNIPRNVSGKIKDRIPDLPIIALARAEAKLELGDPAAETEWRPVVRKEDRERWN
                     DWGIGLLLQGDLKGAEYAFQRVTEAEPGYADGWLNVARALIQEGETEAAKPYIERALK
                     INPKLGRIYYFKALAQKADGDYDGALASLRTAASMYPRDRVVLNQIGRVLFLKRDFRG
                     AVETLERVLTVDPEDVQAQYNLMLSYRGAGENEKAARAEKLFRRFKADEAAQEITAKP
                     RLLSPEDNNERQAIHDHETVPLD"
     gene            3560..>4585
                     /locus_tag="VLE22_10395"
     CDS             3560..>4585
                     /locus_tag="VLE22_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSB14861.1"
                     /translation="MIKPLSAILIAGVFAPLAAAPGVQFTDVTAEAGIKFIHNSGRAG
                     KKWLPETMGSGCAFFDADGDGWLDIILINGRDWTPRGRKPLLALYRNNRNGTFTDVTA
                     ASGLAIEMYGMGVAAGDYDNDGRDDLYITALEGDRLFRNLGGFKFQDATAQSGIRNAD
                     FGTSAAWLDYDRDGKLDLFVANYVKWTSKGDLWCSLDGATKSYCTPESYKGTSSKLYR
                     NLGGGRFEDASARAGVANPTSKSLGVAVLDYDGDGWPDLFVANDTQPNKLYRNNRNGT
                     FTEEGMAAGVAFGEDGAARGAMGVDAADYDRSGRPHLLVGNFSNQMLGLYHNEGNGLF
                     VDEAPRSA"
BASE COUNT          894 a         1370 c         1515 g          806 t
ORIGIN      
        1 atcgacgtaa atggagcggc cgccgccggg caggttggcg gtagctttcc acgatttgcc
       61 ccaatcctgg gagacgaaga actgcgaggt tttgccctgc tgcattacag cgtagagcac
      121 gtgggcatcg ccgggttcga cggcgagcgc ctcaatggtg cccggcaaag acgcggaagt
      181 ggcaatgttg ttcgaagcgt gatcgtcgtt ttgaaagacc ccggtgacga tcgccggatc
      241 aggataaaca agcttccagg tgtcaccggc atcgacgctg cgccaaagcg cacgaccctt
      301 gccaagatac cccccagagg tgcgcgcgta gtacgttcgc gcatcattcg ggtcaaaaat
      361 aacggacctg acaacgccgc ccaggttgta catgcgccac gaggagccac tgtcgtggga
      421 gatataggcg ccggacatgt cagaagcgaa cagaatgtcg tgcggatttc cgcggctcac
      481 ggacggaaac atcatcgtgc ctccgccacc cgggccgatg acctcccagg catcatggcg
      541 ctgtccgggg gcagcgatgg ccacgaaaac cacaaaggcg agtatgtttt tgcgggtcac
      601 cctggtttta tatcgcagac gggcccaaaa atgtggacgg caacgagcgt cagtaatgga
      661 ttcatatgat tggcacctgg gaagcggtcg cgatacgata gatgggcaga acggttcacc
      721 atcagagggg cgaacttaca cgacatgagg ggtcggagac tcaccatttg cgtggcggca
      781 gcttttctgc tgctcctgtt gaataccgcg tacgtttggg cgttcgcgtc gcccaccgtg
      841 ttctacatga gcagcgtgct gatgcacgtg gtcctcggca ttgcggtcgc gattggagcc
      901 ctggttctgg ttcgattgct cccaacaacc gccgccggcc tgctgggagt ttcggcgtta
      961 ctcggcctct atctggcgat ttcggcgcta cccggactcg atctcgtgta cgcgggggcg
     1021 accagagcga atcgcccgat cttgtgggcg cacatcgcca cggcgatcct cggcgttatc
     1081 ctgctcatcc catggatcag taggcgggcg gcggcccagg gaggcggatg gctgcgcttc
     1141 cggaaggctc tgcaaatctg tctggcgctg ctcgtgttgc tgcccgcgtc gacggctgca
     1201 taccggaagt tgttccccaa cccgatggat cgtgttcgga accccatggt cgtgccgacc
     1261 acgatggcgg aagaaggcgc aggcccgaag tccccgttct ggccctcttc agccaacact
     1321 aacgtcaacg gtttgatccc ggcgaatttc ttcctcgatt cgaagctctg cggcgagtgt
     1381 cataaggaca tctacaagca gtggctcggc tcgatgcacc ggttttcgtc attcaacaac
     1441 cagttctacc ggaagtcgat cgagcacatg cagagcgtga tcggcacgaa gccgagccgc
     1501 tggtgcgccg ggtgccatga ccacgcgatg ttcttcaacg ggcgtttcga ccgtccggcg
     1561 atcgaacaga tcgacacgcc cgaagcccag gcgggactgg cttgcgtctc ctgccattcg
     1621 atcgttcacg tgaatagctc gatgggtaac ggcggcttcg tgatcgcgta cccgccgttg
     1681 caccacctgg cgagcagcca gaaccgctac atccggatgc tggaccgttt tctcacgtac
     1741 ctgaatccgg aaccgcaccg gagaaccttc ctgaagccct tcatgcggca gaactcgccg
     1801 gagttctgct ccacttgcca caaggttcat ctcgacgtgc cggtgaacaa ctaccgctgg
     1861 ctgcgcggtt tcaacgacta cgacaattgg caggcgagcg gggtctccgg gcaaggcgcc
     1921 cgctcgttct actacccggc ggagtcgaaa acgtgcgtgg actgccacat gccgctggtg
     1981 ccctcgaagg acccgggcca ccacgacgac ggcaaggtcc actcgcacgc ctttccggcg
     2041 gccaacatgg cggtggcgca tgcgaaccag gatcaggagc agatgaggtt gaccgaacag
     2101 ttcctgaagt cgggcttcat cacggtggac atcttcgccg cctcaccggt ggatgagcag
     2161 ccggggcaat cgccgatgtg gcgccgctcc ggggaggggc cgcagctcat gtccacgttc
     2221 gctgtcggcg aggaagcgga gcaggcgggg caggtgtttc tgcgcgaggt cggaaagctc
     2281 gcggcgccaa tcgaccaggc gggagcgaga ttccggccgg gatcgacggt ccgcgtggat
     2341 gtggtggtgc gtacgcgggc catcggccat ttcttccctg gcgggacggt ggacgcgttc
     2401 gacgtgtggg tggaactacg gggcaaggac gcggacggcc gggtgatttt ttggagcgga
     2461 cgggtagaag acggcggcaa agggccggtg gagaagggcg ctcatttcta ccgttcgtac
     2521 atgatcgacg gcgcgggcaa ccagatcaat aagcgaaatg cctggcaggc gcggagcgtg
     2581 ctgtacgtcc gcctgattcc gccgggcgcc gctgataccg cccacttccg cgtcgcgatc
     2641 ccgaagaaag ctcgaggtcc gatcacgctg gaagcgaagc tcaactaccg gaagttctcc
     2701 cactattaca cgcagttcgt ttacgcggga cagccggagc cgggccaacc tccggagctt
     2761 gtgaatgcga accacgacag ccgggcgtac ttcttcagcc cgtcgaacat cccgaggaac
     2821 gtgtcaggca agatcaagga ccggattccg gacctgccga tcatcgcgct ggcgcgggcg
     2881 gaggcgaagc tggagctcgg cgaccctgcc gccgagactg agtggcggcc ggttgttcgc
     2941 aaggaagacc gggagcgctg gaacgactgg ggcatcgggc tgctactcca gggcgacctc
     3001 aagggcgccg aatacgcctt ccagcgggtt accgaggcgg agcccggcta cgcggacggc
     3061 tggctcaacg tcgcgcgggc gctcatccag gagggcgaaa ccgaagcggc gaaaccgtac
     3121 attgagcgtg cactgaagat caacccgaag ctgggccgga tctactactt caaggctctg
     3181 gcacagaaag cggacggtga ctacgacggc gcactggcct cgctgcgcac ggcggcctcg
     3241 atgtacccgc gcgaccgcgt ggtgttgaac cagatcggcc gggtcctgtt tctcaagcgt
     3301 gacttccgcg gggcggtaga gaccctcgaa agggtcctga cggtggatcc ggaggacgta
     3361 caggcgcaat acaacctgat gttgagctac cggggcgcgg gggagaacga aaaggcggcc
     3421 cgggcggaga agctcttccg gcggttcaag gcggatgagg cggcgcagga gattaccgcg
     3481 aagccgcgcc tgctcagtcc cgaggacaac aacgagaggc aggccatcca cgaccacgag
     3541 accgttccac tggactgaca tgatcaagcc gctttccgca attctgattg ccggtgtgtt
     3601 tgcgccgctc gccgccgcac ccggggtgca attcactgac gtgaccgccg aagccggcat
     3661 caagttcatc cacaacagcg gcagggcggg gaagaagtgg ctgccggaga cgatgggttc
     3721 cggctgcgcg tttttcgacg cggacggcga cggctggctg gacatcatcc tgatcaacgg
     3781 cagggactgg acgcctcgcg ggcggaagcc gctccttgcg ctttaccgga acaaccggaa
     3841 cggaactttt acggatgtaa ccgcggccag cgggctcgcg atcgagatgt acggcatggg
     3901 cgttgccgcc ggcgactacg acaacgacgg ccgggatgat ctttacatca ctgcgctcga
     3961 gggagaccgg ctgttccgca atctcggcgg gttcaagttt caggacgcga cggcgcagtc
     4021 tggaatccgc aacgccgatt tcggcaccag cgcggcttgg ctcgattacg atcgcgatgg
     4081 gaagctggat ctctttgtcg ccaactacgt caagtggacg tccaaaggcg acctgtggtg
     4141 ctcgctggac ggcgcgacga agtcgtactg cacgccggaa tcgtataagg gcacatcgtc
     4201 gaagctctac cggaacctcg gcggcgggcg gttcgaagat gcgagcgcgc gagcgggcgt
     4261 cgcgaacccg acgagcaagt cgctcggcgt cgccgtgctc gattatgacg gggacggctg
     4321 gccggatctt ttcgtcgcca acgatacgca gcccaacaag ctgtaccgga acaaccggaa
     4381 cggtaccttt accgaggagg ggatggcggc gggcgtggcg tttggcgaag atggggcggc
     4441 ccgaggggcg atgggagtcg acgccgccga ctatgaccgc tcgggacggc cgcacctgct
     4501 cgtcggcaac ttctcgaatc agatgctcgg gctgtaccac aacgagggta acgggctgtt
     4561 cgtcgatgaa gccccgcgct ccgcg
//
LOCUS       DATIFQ010000221        10006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_72384, whole genome shotgun sequence.
ACCESSION   DATIFQ010000221 DATIFQ010000000
VERSION     DATIFQ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72384"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2519)
                     /locus_tag="VLE22_10400"
     rRNA            complement(<1..2519)
                     /locus_tag="VLE22_10400"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(2746..2821)
                     /locus_tag="VLE22_10405"
     tRNA            complement(2746..2821)
                     /locus_tag="VLE22_10405"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2786..2788),aa:Ala,seq:tgc)
     gene            complement(2831..2907)
                     /locus_tag="VLE22_10410"
     tRNA            complement(2831..2907)
                     /locus_tag="VLE22_10410"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2871..2873),aa:Ile,seq:gat)
     gene            complement(2983..4520)
                     /locus_tag="VLE22_10415"
     rRNA            complement(2983..4520)
                     /locus_tag="VLE22_10415"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(4842..6050)
                     /gene="tyrS"
                     /locus_tag="VLE22_10420"
     CDS             complement(4842..6050)
                     /gene="tyrS"
                     /locus_tag="VLE22_10420"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSB14862.1"
                     /translation="MMLAPGQQLEELRRGAAELLTEADLKRKLARGKPLRVKAGFDPT
                     APDLHLGHTVLINKMRQFQQLGHTAIFLIGDFTGMIGDPSGKNATRPRLTPGEVQANA
                     RTYQEQIFKILDPEKTVIDFNSRWFGAMSAAGMIELAAKYTVARMLERDDFAKRYRSN
                     QPIAVHEFLYPLVQGYDSVALKADVELGGTDQKFNLLVGRALQADYGQEPQVVLTMPL
                     LEGTDGVQKMSKSLNNYIGIHEPAGDMFGKLMSISDELMWRYFDLLSARTAMDIRKLR
                     KSVQEGRNPRDVKFELAVEIVDTYHGRGAGAAERETFIARFRDGALPEAIPEKTLACK
                     DGSMKLANALKGAGLATSSTAAYRLIEQGAVRVDGARVGSRDAVLDTGSTYLLQAGKR
                     AFARVTLKRS"
     gene            6239..7585
                     /locus_tag="VLE22_10425"
     CDS             6239..7585
                     /locus_tag="VLE22_10425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSB14863.1"
                     /translation="MTRPFLTKATAEVVAVAEPRRLGLIASCVVSGAILSAAGAQLRN
                     PLLDPEAGNLLPAVVQSAAADSAAPATPFEFIAVTIGRNDTLDQVFRGLQLKLSDLAE
                     LRSLPDVRKSLDMIRPGDVIQMTHLNGEIKSLTRRINETATLSVTRPDGVFSASIVEN
                     PLETEETTLRGTVDSSLYVAVNAAGGTDRLAVELADVFKYDIDFVNQVQPGDHFIVAH
                     EQQYQDGAFVRDGDILAAEFVNSGKTYRAVRYVGPNGRADYYTPDGRPVRKAFLRYPV
                     DYARISSGFNLARRHPVLNRVRAHKGIDFAAPSGTPIKAAGAGRVVSRGRNGGYGNVV
                     VLAHSKGVTTLYGHMSRFAKGLSVGDHVAQGQVIGYVGMTGLATGPHVHYEYRVNGVH
                     KNPARVTVPKADPIPASLMADFKAQTTPLLARLDAQSTPAVQVASATQSTGDSAGR"
     gene            complement(7479..8003)
                     /gene="erpA"
                     /locus_tag="VLE22_10430"
     CDS             complement(7479..8003)
                     /gene="erpA"
                     /locus_tag="VLE22_10430"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HSB14864.1"
                     /translation="MSETTHRNDGLIFTDAAARKVGELIREEGNPKLKLRVFVSGGGC
                     SGFQYGFTFDEKIEDGDAEFPNQGVTLLVDPLSFQYLAGAEIDYREDLEGAQFVIRNP
                     NATTTCGCGSSFSTGEKEKKGTLPDFRGGNRGASPLCSSGRRCRRCSASRSRPVRPAS
                     TARLGARGAGWSAP"
     gene            complement(8064..8774)
                     /locus_tag="VLE22_10435"
     CDS             complement(8064..8774)
                     /locus_tag="VLE22_10435"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HSB14865.1"
                     /translation="MSENSRIARWKIALLLLGVLALAWGAFELGRASAGYFVVSSMLE
                     RLDLRKRAQALEGENEALRHRVTVAELGQKVDRQAQSDAQQMMGELQAETARQKQELQ
                     FYRGLVVRQYGSGTLRVQELRVRAEEEDRRYRVLITLVQAATRDTIANGTVTFSVEGH
                     RGKSPATLALPEIEIDKRKQFPFSLRFFQQIEVPIELPVDFEPERLHVEYRLGRNPEP
                     AKQTFDWRVEGGADLPPL"
     gene            8823..9239
                     /locus_tag="VLE22_10440"
     CDS             8823..9239
                     /locus_tag="VLE22_10440"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HSB14866.1"
                     /translation="MHWIKAWHVVFMVTWFAGLFYLPRLFVYHATTKDSAGLARFCVM
                     ERRLFAIMTIGAVLTAVFGMWLLAKDPLLLQTGWLRAKLTLVLLLIGYHAWLWKLVQD
                     FAADRNRHGERWYRWFNEIPSLLLIGIVLLAVARPF"
     gene            complement(9236..9799)
                     /locus_tag="VLE22_10445"
     CDS             complement(9236..9799)
                     /locus_tag="VLE22_10445"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HSB14867.1"
                     /translation="MDRETDYIDADGYRANVGIILMNDDRQVFIGGRVGARGWQFPQG
                     GIRRGERPEQALYRELTEEVGLRPDQVEELARTRSWLRYRLPQQFVRRDSSPPCIGQK
                     QRWYLLKLGVGDSALRFDATEQPPEFDRWRWVDWWDAVHDVIYFKRRVYSRALHELGR
                     FAFPEGLPPYPPWWSERMGARTAGPET"
BASE COUNT         1736 a         3339 c         2888 g         2043 t
ORIGIN      
        1 gccccaggat gtgatgagcc gacatcgagg tgccaaactc ctccgtcgat gtggactctt
       61 gggaggaata agcctgttat ccccggagta ccttttatcc gttaagcgat ggcccttcca
      121 tacagaacca ccggatcact atgacctgct ttcgcacctg ctcgacttgt tagtctcaca
      181 gtcaagcacc cttgtgccat tgcactctgt gcgcgatgac cgaccgcgct gagggtacct
      241 ttgcactcct ccgttactct ttaggaggag accgccccag tcaaactgcc caccatacgc
      301 tgttcccggc ccggataacg ggcctaggtt agaactccga acgcaccagg ctggtatttc
      361 accgttgcct ccacccgggc tagcgcccag gcttcaaagg ctcccagcta tgctacacag
      421 gcaagttcaa agttcagcgt aaagctacag taaaggttca cggggtcttt ccgtctttcc
      481 gcgggaacgc cgcatcttca cagcgatttc aacttcactg agtctcgggt cgagacagtg
      541 gggccagcgt tacgccattc gtgcaggtcg gaacttaccc gacaaggaat ttcgctacct
      601 taggaccgtt atagttacgg ccgccgttta ccggggcttc gatcagaagc ttcgcttgcg
      661 ctgacctcat cacttaacct tccggcaccg ggcaggcgtc agaccctata catccacttg
      721 cgtgttcgca gagtcctgtg tttttgataa acagtcgcag cccccgatta cctgcgacct
      781 ctcgcagctc gtggagcaag tccactcacc acaagaggca caccttctcc cgaagttacg
      841 gtgtcaactt gcagagttcc ttaacccgag ttctctcaag cgccttagga ttttctcctc
      901 acccaccagt gtcggtttac ggtacggccg tttgctgcct gaagcttaga ggcttttccc
      961 ggaagcgggg catcaatcac ttcggtccag taggacctcg tcgtcacgcc tcggaattaa
     1021 cggacctccg gattttccag gagatccttc ctacacgctt gaaccgggca atccgatccc
     1081 ggctgatcta gccttctccg tcaccccatc gcagcagcac ccggtgcagg aatgttgacc
     1141 tgctttccat cgactacgcc tttcggcctc gccttaggtg ccgactcacc ctgctccgat
     1201 taacgttgag caggaaacct tgggttttcg gcgaacgggt ttttcacccg ttttaacgtt
     1261 actcatgtca gcattcgcac ttccgatacc tccagcgtgc ctcgcgacac accttcgcag
     1321 gcttacggaa cgctcctcta ccacttggcc ttgcggccaa atccacagct tcggtgcacg
     1381 gcttgagccc cgcgaaatct tcggcgcagg ccgactagac cagtgagcta ttacgctttc
     1441 tttaaaggat ggctgcttct aagccaacct cctggctgtc tatgacttcc cacatccttt
     1501 cccacttagc cgtaactttg ggaccttagc tggtggtctg ggttatttcc ctcttcacga
     1561 cggacgttag cacccgccgt gtgcctcccg agcttccact ttccggtatt cgcagtttgc
     1621 acaggatcgg taggacggga tgtcccccta gcctgaacag agctctaccc ccggaagtga
     1681 tactcgaggc gatacctaaa tatctttcga ggagaaccag ctatcgccag ccttgattag
     1741 cctttcactc ctatccacac ctcatcgact acttttgcaa cagtagttcg ttcgggcctc
     1801 cagcaggtat taccctacct tcaccctgga catggataga tcgactggct tcgggtctac
     1861 tcccagcgac ttggcgccca tttaggactc ggtttcccta cgcctcccct atgcggttag
     1921 gctcgccact gaaagtaact cgctgaccca ttatacaaaa ggtacgccgt caccccttgc
     1981 ggggctccga ctgcttgtac gcacacggtt tcaggttcta tttcactccc ctctccgggg
     2041 ttcttttcgc ctttccctca cggtactagt tcactatcgg tcggcagaga gtatttagcc
     2101 ttggaggatg gtccccccat gttcagacag gattacacgt gtcccgccct actcgtcgtc
     2161 actcatcgga cttccctttc gcgtaccggg ctatcaccgt ctatggccgg gctttccatc
     2221 ccgttctgct agggtcatcc gactgatcgt gacagggctg atccgcgttc gctcgccact
     2281 acttgcggaa tctcggttga tttctgttcc tccggttact gagatgtttc aattcaccgg
     2341 gtttgcttcg cgcgcctatt tattcagcgc gcgataccgc ctatgcggtg ggtttcccca
     2401 ttcggaaatc cccggatcaa agccagtttg ccggctcccc gaggcttatc gcaggctacc
     2461 acgtccttca tcgccttctg ccgccaaggc atccaccatg tgcgcttagt cgcttgacca
     2521 tataacccca atgagcctgg ggtcacttac cttgctgatc tctcgcgaat gcgacacata
     2581 ctacaaaacg cgatttcaaa aaaattcgtc gcgttgatgc attgcgtttt ccacattccg
     2641 tcagattttt aaagagcggg ggcgcctgtg cgcccgtgcg atcggcgctg cactcgaatc
     2701 aagtgcagtg cacatcacac gggtcgacga acctcggagg aaagctggtg gagccaatcg
     2761 ggatcgaacc gatgacctcc tgcttgcaaa gcaggcgctc tcccagctga gctatggccc
     2821 catcaagatt tggtgggtct gggaagagtt gaacttccga cctcaccctt atcaggggtg
     2881 cgctctaacc aactgagcta cagacccggg gttcggcttg ggcgcttgat gcaggtaatt
     2941 tgaatgggaa ctcgcgccgg atggtccggg cgcggatctc ttaaaggagg tgatccagcc
     3001 gcacgttccc gtacggctac cttgttacga cttcacccca gtcattggcc acaccgtggt
     3061 aagcgccctc cttgcggtta ggctacctac ttctggtgca accaacttcc atggtgtgac
     3121 gggcggtgtg tacaagaccc gggaacgtat tcaccgcagc aatgctgatc tgcgattact
     3181 agcgattccg acttcacgga gtcgagttgc agactccgat ccggactacg aacggctttt
     3241 tgggattggc tccccctcgc gggttggcaa ccctttgtac cgcccattgt agcacgtgtg
     3301 tagccctggc cataagggcc atgatgactt gacgtcatcc ccaccttcct ccggtttgtc
     3361 accggcggtc tccttagagt tcccaactga atgatggcaa ctaaggacaa gggttgcgct
     3421 cgttgcggga cttaacccaa catctcacga cacgagctga cgacagccat gcagcacctg
     3481 tctcgcgatt cccgaaggca ccaaggcatt tctgccgagt tccgcggatg tcaaggccag
     3541 gtaaggttct tcgcgttgca tcgaattgaa ccacatgctc caccgcttgt gcgggtcccc
     3601 gtcaattcct ttgagtttca accttgcggc cgtactcccc aggcggacga cttaacgcgt
     3661 tagctacgac accgaaaggc agacccctcc gacatccagt cgtcatcgtt tagggcgtgg
     3721 actaccaggg tatctaatcc tgtttgctcc ccacgctttc gcacttgagt gtcagtactg
     3781 gaccagaggg ccgccttcgc cactgatgtt cctcccgata tctacgcatt tcaccgctac
     3841 accgggaatt ccgccctcct cttccgtact ccagcaatgc agtctcgaat gcaattccca
     3901 ggttgagccc ggggctttca cacccgactt acgtcgccac ctacgtgcgc tttacgccca
     3961 gtaattccga ttaacgctcg caccctctgt attaccgcgg ctgctggcac agagttagcc
     4021 ggtgcttctt cattgagtaa cgtcagcttg ccggggtgtt agcccggcac atttcgttcc
     4081 catcgaaagt gctttacaac ccgcaggcct tcttcacaca cgcggcgtcg ctggatcagg
     4141 ctttcgccca ttgtccaata ttccccactg ctgcctcccg taggagtctg ggccgtgtct
     4201 cagtcccagt gtggctggcc gtcctctcag accagctacg gatcgtcgcc ttggtgagcc
     4261 gttacctcac caactagcta atccgacatg ggctcatcca tcggcgcgag gccttacggt
     4321 cccccgcttt catccgaaga tctcatgcgg cattagcccg gctttcgccg ggttattccc
     4381 caccaaaggg cagattccca tgtattacgc acccgttcgc cgctcgtcag cacgtattgc
     4441 tacgcctgtt accgctcgac ttgcatgtgt taagcacgcc gccagcgttc aatctgagcc
     4501 aggatcaaac tcttcagttc aatggtttga gctgaagcga gatcccaaac tccaaacatg
     4561 tccggtctta cccggacggc tggacgcttg gttactcgca gtggcgccca agatggacac
     4621 ctgacgcgag tccccacaca aattacctgc aaatttttaa agaacacctg gtcgacaaaa
     4681 gcgcccaggg cgaccgcaaa ttttaccccg cgtcaggggt cccgtacaac ataagtcatg
     4741 ccgcacgaac ccgcccgggc catcctttcc aggaccgccc gaggtcgggg ggcgcgcagt
     4801 atagcccccc gattggggga gtcaacggct tttggccccc atcaggagcg cttgagagtc
     4861 actcgggcaa aagcccgttt tccagcctgc aggagatagg tggaaccggt atcgagcacc
     4921 gcatcccggc tgccgacacg cgcgccgtcg acccgcacgg cgccttgttc gatgagccgg
     4981 tatgcagcgg ttgaactcgt cgcgaggccc gcgcccttga gcgcgttcgc cagcttcatc
     5041 gagccgtcct tgcaggcgag cgtcttctcc ggaatggcct ccggcagcgc cccgtcgcgg
     5101 aagcgggcga tgaatgtctc ccgctctgcc gcgccggcac cgcggccgtg ataggtgtcc
     5161 acgatctcga cggcgagctc gaacttgacg tcccggggat ttcggccctc ttgcacggac
     5221 ttccgtagct tccggatgtc catagccgtc cgggctgaca gcagatcgaa gtaccgccac
     5281 atgagctcgt cggaaatgga catgagcttg ccgaacatgt ccccggccgg ttcgtggatg
     5341 ccgatgtagt tgttgaggga cttcgacatc ttctggacgc cgtccgtccc ctcaaggagc
     5401 ggcatggtga gcacgacctg cggctcctgg ccgtagtcgg cctgcagcgc ccgcccgacc
     5461 agcaggttga atttctggtc cgtgccgccg agctcgacgt cggccttgag cgccaccgag
     5521 tcgtagccct ggaccagcgg gtagaggaac tcgtgcaccg cgatcggctg gttcgatctg
     5581 tagcgcttgg cgaagtcgtc gcgctcgagc atgcgcgcga ccgtgtattt cgcggcgagc
     5641 tcgatcatgc cggccgcgct catggccccg aaccagcgcg agttgaagtc gatcacggtc
     5701 ttctcggggt ccaggatctt gaagatctgc tcctggtagg tgcgggcgtt cgcctgtacc
     5761 tcccccggcg tcagccgcgg gcgggtcgcg ttcttgcccg aggggtcgcc gatcatcccg
     5821 gtgaagtcgc cgatcaggaa gatcgccgta tgccccagct gctggaactg gcgcatcttg
     5881 ttgatgagca ccgtatggcc gaggtgcagg tcgggagctg tggggtcgaa gcccgccttg
     5941 acccggagcg gcttgccgcg cgcgagcttc ctcttaaggt cggcctcggt caggagctcg
     6001 gcggcgccgc ggcggagttc ttccagttgt tgtcccggcg ccagcatcat ccccccttgc
     6061 cggcagccta ctgcggcagg cctgcaattc tgtgaggcgg gcatggcctt ggcaatcgcc
     6121 ggacccggcc gcgcggccta tatatgtgac ggagtacaaa ggccacccaa tcaacaggtt
     6181 gacctgactt cctgcattga tttaccgtcc cgcttctacc tcccggggat acccgctggt
     6241 gacacgaccc ttcctgacga aggccaccgc tgaagttgtc gccgtcgccg agccgcggcg
     6301 gctcggcctt attgcctcct gcgtcgtttc gggcgcgatt ctctcggccg cgggcgccca
     6361 gctgcggaac cccctgctcg acccggaggc gggcaacctc ctgccggcgg tcgtacagtc
     6421 ggcggctgca gacagcgcgg ccccggcgac gcctttcgag ttcatcgcgg tcacgatcgg
     6481 ccgcaatgac acgctcgacc aggtcttccg cgggctgcag ctgaagctct ccgacctcgc
     6541 cgagcttcgg agcctcccgg acgtccgcaa gagcctcgac atgatccgcc cgggcgacgt
     6601 catccagatg acacacctga acggcgagat caagtcgctc acccgccgca tcaacgagac
     6661 cgcgacgctc tccgtgacgc gccccgacgg cgtcttcagc gccagcatcg tcgagaaccc
     6721 gctcgagacc gaggaaacga cgctccgcgg cacggtggac agctcgctct acgtggcggt
     6781 caacgcggcg ggcggcaccg accgcctcgc ggtcgagctc gcggacgtgt tcaagtacga
     6841 catcgatttc gtcaaccagg tgcagccggg cgaccacttc atcgtggcgc acgagcagca
     6901 gtaccaggac ggtgcgttcg tgcgcgacgg cgacatcctc gcggccgaat tcgtgaattc
     6961 cggcaagacc taccgcgcgg tgcgctacgt cggcccgaac gggcgcgcgg actactacac
     7021 gccggacggc cgcccggtgc gcaaggcctt cctgcgttac ccggtcgact acgcccgcat
     7081 cagctcgggc ttcaacctgg cgcgacggca cccggtgctg aaccgcgtgc gcgcgcacaa
     7141 gggcatcgat ttcgcggcgc cctcgggcac gccgatcaag gcggcgggcg cgggccgcgt
     7201 cgtcagccgg ggccgcaacg gcggctatgg caacgtcgtc gtgctcgcgc acagcaaggg
     7261 cgtcacgacg ctctacggcc acatgtcgcg cttcgcgaaa ggactttcgg tcggcgacca
     7321 cgtcgcccag gggcaggtca tcggctacgt cggcatgacg ggcctcgcga ccggcccgca
     7381 tgtgcattac gagtaccgcg tgaacggcgt acacaagaac ccggcgcgcg tgacagtgcc
     7441 gaaggccgac ccgatcccgg ccagcctgat ggcggacttc aaggcgcaga ccaccccgct
     7501 cctcgcgcgc ctagacgcgc agtcgacgcc ggccgtacag gtcgcgagcg cgacgcagag
     7561 caccggcgac agcgccggcc gctagagcaa agaggggacg ctccccgatt tccgccgcgg
     7621 aaatcgggga gcgtcccctt tttctctttt tctccagtcg agaacgacga cccgcagccg
     7681 caggtcgtgg tggcgttcgg gttgcggatg acgaactgcg caccctcgag gtcctcgcga
     7741 tagtcgatct ccgcgcccgc gaggtactgg aacgagagcg gatcgacgag cagcgtcacg
     7801 ccctggttgg ggaattccgc gtcgccatcc tcgatcttct catcgaaagt gaatccgtac
     7861 tggaagcccg agcagccgcc gccggagacg aacacgcgca gcttcagctt cgggttgccc
     7921 tcctcgcgga tgagctcgcc gaccttgcgc gcggccgcgt ccgtgaagat gaggccatcg
     7981 ttcctgtgcg tcgtttcgct cacgaattcc tttccaccgt ccggatgatc agcgtttcat
     8041 cttaggacct cgccggcggg gggtcaaagc ggcggcagat ccgcgccacc ctcgactcgc
     8101 cagtcgaaag tctgcttggc cggctcgggg ttgcggccga gccgatactc gacatggagg
     8161 cgctccggct caaaatccac cggcagctcg atcggcacct cgatctgctg gaagaagcgc
     8221 aacgagaacg ggaactgctt gcgcttgtcg atctcgatct cgggcagcgc cagcgtcgcg
     8281 ggcgacttgc cccggtggcc ctcgaccgag aaggtgacgg tgccgttcgc gatcgtgtcg
     8341 cgggtcgcgg cctggaccag cgtgatgagc acgcggtagc gccggtcctc ctcttccgcg
     8401 cgcacgcgca gttcctgcac gcgcagcgtc ccggagccgt actggcgcac gaccaggcca
     8461 cggtagaact ggagttcctg cttctggcgc gccgtctcgg cctgcagttc gcccatcatc
     8521 tgctgcgcgt ccgactgcgc ctggcgatcc accttctggc cgagctcggc gaccgtcacg
     8581 cggtggcgga gcgcctcgtt ctccccctcg agcgcctgcg cgcgcttgcg caggtccagc
     8641 cgctccagca tcgagctcac gacgaagtag cccgcgctcg cgcgcccgag ctcgaaggcg
     8701 ccccaggcca gggcgaggac cccgaggagc agcagggcga tcttccagcg ggcgatgcgg
     8761 ctgttctcgc tcaaggccgg acgtcgtcgc tagaatcgcg gcgggaacgg gggcacggac
     8821 gaatgcactg gatcaaggcc tggcacgtcg tcttcatggt gacctggttc gcgggcctct
     8881 tctacctgcc gcggttgttc gtataccacg ccacgacgaa ggacagcgcg gggctcgcgc
     8941 ggttctgcgt gatggagcgc cgcctgttcg cgatcatgac gatcggcgcg gtgctgacgg
     9001 cggtcttcgg catgtggctc ctggcgaaag acccgctcct gctccagacc ggctggctgc
     9061 gggccaagct cacgctcgtc ctcctgctca tcggctacca tgcctggctc tggaagctcg
     9121 tgcaggactt cgcggcagac cgaaaccggc acggcgagcg ctggtaccgc tggttcaacg
     9181 agatcccctc gctgctcttg atcggcatcg tgctgctcgc ggtcgcgcgg ccgttctagg
     9241 tttcgggccc cgcggtgcgg gcgcccatgc gctccgacca ccacggcgga tagggcggca
     9301 gcccctccgg gaacgcgaac ctgccgagct cgtgcagcgc gcgcgagtac acgcggcgct
     9361 tgaagtagat gacgtcgtgc acggcgtccc accagtcgac ccagcgccag cgatcgaatt
     9421 ccggcggctg ctcggtcgcg tcgaagcgca gcgccgagtc cccgaccccg agcttcagga
     9481 gataccagcg ctgtttctgg ccgatgcagg gcgggctcga atcgcggcgc acgaactgct
     9541 gcggcaaccg gtagcgcagc cagctgcgcg tgcgtgcgag ctcctcgact tgatcggggc
     9601 ggagccccac ctcctccgtg agctcgcggt agagcgcctg ctcgggccgc tcgccgcggc
     9661 ggatgccacc ctgcgggaac tgccagccgc gcgcgcccac gcgcccgccg atgaacacct
     9721 ggcggtcgtc gttcatcagg atgatgccga cgttcgcgcg gtagccatcg gcgtcgatgt
     9781 agtcggtctc ccggtccatg ggcgcgattt taatcggcta gagtgcgcgc atggggagga
     9841 tgcgcgcgac gaccctggtc ctgctgctga tcgctgcgtc catcgtcgcg atcgcggctt
     9901 cgctcgccct cggcagttcc ggcatcgacg cgccctggga gcggctcgcg gcgggcgacc
     9961 cgttgatgcg cacgatcgtc tgggagctgc gcctcccgcg ggcgct
//
LOCUS       DATIFQ010000222         4215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1222615, whole genome shotgun sequence.
ACCESSION   DATIFQ010000222 DATIFQ010000000
VERSION     DATIFQ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222615"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VLE22_10450"
     CDS             complement(<1..239)
                     /locus_tag="VLE22_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB14868.1"
                     /translation="MTDRPSYGTAGKLAANFIQSKLTPLFIVASLALGAFAVIKLPRE
                     EEPQIIVPMIDVFVQMPGASAKEVEERVTRPMEKLL"
     gene            complement(236..1390)
                     /locus_tag="VLE22_10455"
     CDS             complement(236..1390)
                     /locus_tag="VLE22_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB14869.1"
                     /translation="MRKRLFPLIVVVLLGVLTACGEKPRRSEAASSATPVAVQTVTVK
                     STEWPQTYEAVGTVRARTSTVISAKVMGYVREVRFQTGDAVRQGQLLVALDSRDLDAH
                     YRQAEAAVGEAESAMPEVESAMAAAKANLDLAQVTFHRMKELFDKKSISNQEYDESSA
                     RLKEAQANYAMATAKREQLTSKIAQAQEGFQSAGVMRGYSQIVAPFDGVVVEKQVELG
                     NLAAPGAPLATIERAGALRLEASVSESWIALIRAGHKATVTLDALDKTVESRVSEIVP
                     SVDPAARAYTVKIELPPMSQVRSGMFGRAAFQLGSKQTLVAPAGAVIERGQLQSVMVA
                     DEGVARTRLVTMGQSLQDQRQVLSGLNPGEKVIYPVPAGLVDGARVEVRP"
     gene            complement(1403..2734)
                     /locus_tag="VLE22_10460"
     CDS             complement(1403..2734)
                     /locus_tag="VLE22_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB14870.1"
                     /translation="MKTGLVLLLSVPLFSQTPLSLKDAVRTAIEKHPSVEAAGAQVSA
                     AETRLRQARSGHLPKLNYTESWQRSNNPVFVFSSLLTQHQFGEHNFAVGPLNRPDFLN
                     NFQSQVTVDQMIWDFGQTKTQIRSAELGQSIAGEDKRRTQMEVIANVVRTYYGALLAQ
                     ESLNVAREAVRSAEADEQRADAVRSAGMATDADVLAIRVHLAAMREQAIRRSYDVEIA
                     RAALNEALGLPLDTKHDLSTPLSEFTGESTPLDQREQNASESRPEARQVRLSASIAET
                     QTQAARSALLPQFSVRVAFEADRQEFINKGGANWFAGASMRWNLFNGFADKARIDEAS
                     QSLARARAQQRQIDSAVRLQVRSAYLDLQAAQERLKVTEAAVTQAEENLRIVKNRYEN
                     GLNNVTELLRSETALLETRVRRLAAVHGQRLAAVALDLAAGTLTLDSEVLN"
     gene            2988..3590
                     /locus_tag="VLE22_10465"
     CDS             2988..3590
                     /locus_tag="VLE22_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB14871.1"
                     /translation="MEREQEVELARDLLAGNLEVFDRFVESFRTRLFQYSLLMCGQRE
                     DAEEVSQETLLKVFENLDQLREPEHVRSWVFRIAKNACLMKRRKSVFAPVRELSLDEF
                     IPSMDHHGGERKLEIADWSALPDAQALRSELRNVLQEAISELPEIYKSVLLLRDVEEL
                     STEEAAQVLDVSEDVVKTRLHRARLAVRQKLDQYLKVRVN"
     gene            3600..3896
                     /locus_tag="VLE22_10470"
     CDS             3600..3896
                     /locus_tag="VLE22_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSB14872.1"
                     /translation="MAEQTGKHFGSCKEIFAELSAYLDADLPPDACQEIDEHLSGCAP
                     CVEFVNSLRRTVELCRGYEASSVPGPLTQEARGQLLVAFQQMLAAKKGSPPNRT"
     gene            4009..>4215
                     /locus_tag="VLE22_10475"
     CDS             4009..>4215
                     /locus_tag="VLE22_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14873.1"
                     /translation="MATTVNAMPPEATAERKPAPRKKLLKRRIADHSQTLRRVVQLAF
                     FALNVWIGVEFFLFVRHYETGGASV"
BASE COUNT          754 a         1303 c         1333 g          825 t
ORIGIN      
        1 aggagcttct ccatcggcct ggtgacgcgc tcctccactt ctttcgccga tgcgcccggc
       61 atctgcacaa acacgtcgat catcggaacg atgatctgcg gttcctcttc tcgcggcagt
      121 ttgatgactg cgaaggcgcc gagcgcgagc gaagcaacga tgaacagcgg cgtgagcttg
      181 gactgaatga aattcgcagc cagcttgccg gctgttccgt aagagggacg gtcggtcacg
      241 ggcggacctc caccctggcg ccatccacca ggcctgccgg caccgggtag atcacttttt
      301 cgccggggtt caggccggaa agcacctggc gctggtcctg aagcgattga cccatagtga
      361 cgagccgtgt gcgtgcgaca ccctcgtcgg cgaccatcac cgactggagc tggccgcgtt
      421 cgattaccgc gccggcgggc gccaccagag tttgtttcga gccgagttgg aacgctgccc
      481 gcccgaacat gccgctgcgg acttgggaca tcggaggcag ctcgatcttc acggtgtagg
      541 ctcgcgcggc gggatcgacc gacgggacga tctcggagac acgcgattcg accgtcttgt
      601 cgagtgcgtc gagcgtcact gtcgccttgt gccccgcccg gatcagggcg atccaagact
      661 cggagaccga tgcctcgagg cggagagcgc cggcacgttc tatggttgcc agcggagcgc
      721 ccggcgcggc aaggttacca agctcgacct gtttctccac taccacccca tcaaacggcg
      781 caacgatctg agagtagccg cgcataacgc ccgcggactg gaagccttcc tgggcctgag
      841 ctatcttcga cgtgagttgt tcacgcttcg ctgtcgccat ggcgtaattc gcctgtgcct
      901 ctttgagccg ggccgacgat tcgtcatatt cctgattgga aatcgacttc ttgtcgaaga
      961 gttccttcat gcgatggaaa gtgacttggg cgaggtcgag gttcgccttg gcggcggcca
     1021 tcgcactttc gacctcgggc atagcgcttt ctgcttcacc cactgccgcc tcggcctggc
     1081 gatagtgagc gtccaggtcg cgcgagtcga gcgcaaccag caactggccc tgccggacgg
     1141 cgtcgccggt ctggaagcgg acttcacgca cgtagcccat caccttcgcc gagatgacgg
     1201 ttgacgtgcg cgcgcgcacg gtgccgacgg cttcataggt ctgaggccat tcggtggatt
     1261 tcacagtcac ggtctgaaca gcaaccggcg tggcggatga cgcggcttcg gagcggcgcg
     1321 gcttctcgcc gcaagcggtc agcacgccga gaagcaccac aacgatcagg gggaatagtc
     1381 tttttcgcat ctcaggacgc tcctaattga gaacttccga atccagggtg agcgtgccgg
     1441 cggcaaggtc gagagcgacc gccgccaggc gctgtccgtg gaccgcggcc aaccgccgga
     1501 ccctcgtttc cagcagcgcc gtttcactgc gcagcaattc ggtaacgttg ttcagaccgt
     1561 tctcgtaacg gtttttgaca atccgcaggt tttcctcggc ttgggtcacg gccgcctcag
     1621 tcaccttgag gcgctcctga gcggcctgga ggtccaggta ggcgctccga acctggagac
     1681 gcacggccga atcgatctgg cgctgctggg cgcgtgcgcg ggccagggac tggctcgctt
     1741 cgtcgatgcg cgccttgtcg gcaaaaccgt tgaacaggtt ccagcgcatg gaggcgcctg
     1801 cgaaccaatt ggcgccgccc ttattgatga attcctgccg gtccgcctcg aaggcgacac
     1861 ggacggagaa ctgcgggagc aacgccgacc gagccgcctg agtctgtgtc tcggcgatgg
     1921 acgccgaaag cctcacctga cgggcttcgg gacggctctc gctggcgttc tgctcgcgct
     1981 ggtcgagcgg cgtagattcg ccggtgaact cgctgagcgg cgtgctcaag tcgtgttttg
     2041 tatcgagcgg caggcccagg gcttcgttca gcgctgcgcg ggcgatctcc acgtcgtagg
     2101 acctccggat cgcctgctcg cgcattgctg cgagatgaac gcgaatcgcg agcacgtcgg
     2161 cgtccgttgc catgcccgcc gaccgcacgg cgtcggcccg ttgctcgtcc gcctccgccg
     2221 aacgcaccgc ctcacgggcc acattcaggc tctcttgagc gagcagtgcc ccgtagtaag
     2281 tccggacgac gttcgcgatc acctccatct gcgtgcggcg cttgtcctcg ccggcgatgc
     2341 tctggccgag ctcggcggaa cgaatctggg ttttagtctg cccgaagtcc cagatcatct
     2401 gatcaaccgt aacctgcgac tggaaattgt tcaggaaatc cggccggttc agtggtccga
     2461 cggcgaagtt gtgctccccg aactgatgct gtgtcagcag agaactgaag acgaacaccg
     2521 ggttattgct gcgctgccag gactcggtgt agttcagctt gggcaggtgg ccgctgcgcg
     2581 cttggcgcag ccttgtctca gccgcactca cctgtgcgcc ggccgcctcg acggacgggt
     2641 gcttctcgat ggccgtgcgg actgcatctt ttagcgagag cggcgtctgg gagaacaggg
     2701 ggacgctcaa gagcaaaaca agaccggtct tcatacgatt ccatttcgca tatcttagtt
     2761 gtctctgtta ttggtcagga tgcaggcgtc ggccggaggt aacagacaag gaactccgat
     2821 caccgtccag ccgggcctgc atccccaggc tcgggacacg gaactccgga aaacattcta
     2881 tctgttcgag cctctcttga gaatcgcgcc cccgcgtcct tcgttccttt cccgctacaa
     2941 tcacctgaga aggggatttc tgtcacttcc ccgcccggat ggcatctttg gagagagaac
     3001 aggaagtcga gctggcgcgg gacctcctgg ctggcaacct tgaggtgttc gaccgtttcg
     3061 tggagagttt ccgcacaagg ctcttccagt acagcctgct catgtgcgga cagcgcgagg
     3121 acgccgagga ggtctcccag gaaaccctgc tcaaggtctt tgagaatctt gaccagcttc
     3181 gtgagccgga gcatgttcgc tcgtgggtat tccggatcgc caagaatgcc tgcttgatga
     3241 agcgtcggaa aagcgtgttc gctcccgtcc gcgaattgtc gctcgatgag ttcatcccgt
     3301 cgatggacca ccacggtggc gagagaaagc tggagatcgc cgactggtcg gcgcttccgg
     3361 atgcccaggc gctgcgctcg gagcttcgga atgtcttaca agaagcgatc tcggagctgc
     3421 cggagatcta caagtccgtg ctgctgctcc gagatgtgga ggagctgtcc accgaagagg
     3481 ccgcacaggt gttggatgtg agcgaggacg tcgtcaagac tcggctgcac cgcgcgcgcc
     3541 ttgcggtgcg gcagaagctc gaccagtatc tgaaggtgcg cgtgaactag gagaagccca
     3601 tggcagagca gacgggcaaa cactttggga gctgcaaaga gatcttcgcg gagctgtccg
     3661 cgtacctcga cgccgacctg ccgccggatg cctgtcagga gatcgacgag catctctcgg
     3721 gctgtgctcc ctgcgtggaa ttcgtgaaca gcctgcgccg caccgtggaa ctctgccgcg
     3781 gctacgaggc aagctccgtc cccggcccgt tgacccaaga ggcgcggggc cagcttctgg
     3841 tggcgtttca gcagatgctc gccgcgaaga agggatcgcc gccgaaccgc acgtaagcat
     3901 aagaagcggc tcatcttagt cgtggaaacc tggcgtcttc tctcgtcgct gtgacaccgg
     3961 tcactgccgg gcggcggctc acggcataag gtggtatcag gaacggcgat ggcaacgacc
     4021 gtcaacgcaa tgccccccga ggctacagca gagcgaaaac cggcgccgcg caagaagctg
     4081 ttgaaacggc ggatcgccga tcactcccag acactgcggc gcgtcgtgca gcttgcattc
     4141 tttgctctga acgtctggat cggggtggag ttcttcctct ttgttcgcca ttacgagacc
     4201 gggggcgcct cggtt
//
LOCUS       DATIFQ010000223         5682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1989782, whole genome shotgun sequence.
ACCESSION   DATIFQ010000223 DATIFQ010000000
VERSION     DATIFQ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989782"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /gene="rsgA"
                     /locus_tag="VLE22_10480"
     CDS             <1..556
                     /gene="rsgA"
                     /locus_tag="VLE22_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HSB14874.1"
                     /translation="VPVTVVSALAEQGAAELIRHVRHGETAALVGSSGVGKSTIVNWL
                     LGRDSQPTHEVREHDGRGRHTTTSRELIPMPGGWLLMDTPGLRELQLWAGEDGLDRTF
                     EDVASFAAMCRFRDCRHEGEPGCAVTEAVSGGDLDEARLGSYRKLQRELAHLVRRQDE
                     RAAAEEKRRWKRIHKAMRQGGKRL"
     gene            658..1404
                     /locus_tag="VLE22_10485"
     CDS             658..1404
                     /locus_tag="VLE22_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14875.1"
                     /translation="MKLAAMLAMLLLLPLSATDRRKGIYVGFSDSVLPHIADGNGWKT
                     LITLANMDSKIVQYTLTFRSSPTGQPWQLNIAGFGAITTISGTIPIGGSAFFETTNPP
                     GPTSVGWAQLETYDRIGGMAVFRFSPGGLQDTEAVVPFASEYDDDFLIPFDNRSGFVT
                     SMAIVNPSSYTAVSVFVEFRDVTGQRILLDTITLQPREHRAFSTAERFPASIEKNGVI
                     AFSTADLGVSALGIRFNPRFSFTSVHALSK"
     gene            1457..3217
                     /locus_tag="VLE22_10490"
     CDS             1457..3217
                     /locus_tag="VLE22_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14876.1"
                     /translation="MFFLKWLSLAVLISIPSAAQFEDLSTDDTGSRVWFSSLLRLRGT
                     DQELYDKLFTADANGHVTLLLQRPPDNPYHLLNRPEVSGDGRTLAYLARYYCPPQVTC
                     RVAVGSHGVITVPGQPDIEVTGSIHLSRNGRYIMRDSYRYLGPTDQVEMIDLQTGTRH
                     QIPVANFLPISGGRQVASNGSALVYANGLWLIRFDGSFQFVPTWNFGPSSDPQQWFPA
                     ALSATMDDSASIVVFQTMAGTPGLGIVRPDDSSSLRTLVSAEEGCTNPVMSADGKRVL
                     FLSQANFSGSNTRGLQQAWIVSTDSGAIQMVTNDPAGIEEATLSGDGNAVWASTFAGR
                     LLWIRVDTSVVREIIPWTTAVDSDPLNFPIRAAPGSLAQLTGRGLSSQQVNSGLPLAT
                     ELSGVRLLADGAPLPLLFVSPKEIGFQVPWEMRGRHTLTLAPTRSPFEDQLTRQLQVV
                     DTSVELLNTADGQPAIAHDDFHGLVTPNDPARPGEVLHLYLTGLGAVDSPVATGDAAP
                     MAPLSTVTDRVSVYWAGSVLPAPPPSGPEVLFAGLAPGMVGIYQVDIRLPANAPLAQL
                     GISIQVGDTNTVTTFPVGVP"
     gene            3331..4752
                     /locus_tag="VLE22_10495"
     CDS             3331..4752
                     /locus_tag="VLE22_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14877.1"
                     /translation="MALEEVVRHTPSCMTRRRFGLTLCGVPLSYALAAEDFGAMPWAG
                     PASVAKVYLAGATIHWPKPDLDVQKDIAEIEARLAEFERKHPNDVRFTGGQLLRKEED
                     VAPWAQSIADADAVLILPLAQPLGPLGQVLDAIKVPALYFSRPYAGHSWASIAGYRKS
                     GKRVDAVASSSYGDLDAYAGIFRTIHHLRKSRMLVVANSPRGRDAQAKAYSEYFGTDF
                     RFLTYDDLNAAFADGDERKARQAAEEFTRGALRVVEPKPKEIQDALRFYFGIAGMMKR
                     EKANAVTVDCFGGLLAHKLPAYPCITWSKLNDQGLYGVCEGDIESTMSQMLVTSYSGM
                     PGFVSDPVIDLSRNEIIHAHCVAATKMKGIGGSSSPYIIRNHLETNEGAVLQVLMPSG
                     ETITCGRFATPKKFLVSTAVVTGNVDSDRGCRSQIRTQVTDAQKMLEAYSAGLHRVIF
                     YGDHVKTVERMGRMMGFEVVKEI"
     gene            4906..5445
                     /locus_tag="VLE22_10500"
     CDS             4906..5445
                     /locus_tag="VLE22_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HSB14878.1"
                     /translation="MKSLLEPPPARTEDQDFIDRIIKEQGDRPGALLTVLERLQERHP
                     NKYLPMETLEYVAARTDIPVAQVWSVVTFYALFNLEPQGQHTVCICRGTACHTRGSRS
                     LLERLRLELGVNKKDDQHEADKLSLTTPDRRFTLRTVACFGQCALAPVVEIDHAIYGH
                     MNEQTLHRELQALNQEGAK"
     gene            5442..>5682
                     /locus_tag="VLE22_10505"
     CDS             5442..>5682
                     /locus_tag="VLE22_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HSB14879.1"
                     /translation="MRRITDIASFSEAKAAGMAKLMPLRPRIAVGMGTCGTGNGAESV
                     YQAFASAIDFRGLDAELTRTGCFGFCAQEPLVNVRL"
BASE COUNT         1127 a         1758 c         1676 g         1121 t
ORIGIN      
        1 cgtacccgtg acggtggtca gcgcgttggc ggagcaaggc gccgcggaac tcatccgcca
       61 cgtgaggcac ggcgagaccg cagcgctcgt gggctcttcc ggggtaggga agtcgaccat
      121 cgtgaactgg ttgctcggga gagactcgca gccgacccac gaggtgcggg agcacgacgg
      181 gaggggtcgc cacacgacca caagccgcga gttgatcccg atgccgggcg ggtggctgct
      241 catggatact ccgggcctgc gcgagctgca actctgggct ggcgaggacg gcttggaccg
      301 gacatttgag gacgtggcga gctttgcagc catgtgccgc ttccgcgact gccgccatga
      361 aggcgaaccc ggatgcgccg taacggaagc cgtctccggc ggcgatctgg acgaggcgcg
      421 gctcggaagc taccgtaagc tccagcgcga gctggcgcac ctcgtccgcc ggcaggacga
      481 gagggcagcc gcggaagaga aacggaggtg gaaacgcatc cacaaggcga tgcggcaggg
      541 cggcaagcga ctgtagattc agcgacaatc acctccggta atcctttgcg acgggctgcg
      601 atacaagtca taattctccg ttaaggagac ttcatcatgc gatcgcatac gcactctgtg
      661 aagctggcgg cgatgctcgc catgctcctc ttgcttccgc tttcagctac cgatcgacga
      721 aaaggaatct atgtcgggtt cagcgattcc gtactcccac acattgccga cggaaacgga
      781 tggaagacgc taatcacatt ggccaacatg gattcaaaga tcgttcagta cactttgacg
      841 ttccgttcca gcccgacggg tcaaccgtgg caattgaaca ttgccggatt cggtgcaata
      901 accacaatct ccggaaccat ccctattgga gggtctgcat tcttcgagac cacaaaccca
      961 cccggtccca cgtccgtagg ttgggcgcag cttgagacct acgatcgcat cgggggcatg
     1021 gctgtctttc gattttcccc cggaggcctt caggacacag aagccgtcgt tccgtttgcg
     1081 agcgaatatg atgatgactt tcttattccg tttgacaatc gaagcggatt cgtcacctcg
     1141 atggcaatcg tgaatccgtc ttcgtacact gcggtttcgg tattcgtgga gttcagagat
     1201 gtgacgggcc agcgaatcct gctggataca attacacttc aaccacgcga acatcgtgcc
     1261 ttctcgaccg ctgagcgatt cccagcctct atcgaaaaga acggcgtgat tgcatttagc
     1321 acagccgatc taggagtctc cgcactggga atccggttca atcctcgttt ttcgttcaca
     1381 tcggtccacg ccctgagcaa gtgaacgggg tagcgcccct gtcttggccc gaaatggaga
     1441 atagcatgga aatgccatgt tttttctgaa atggctctcg ttagctgttc tgatctccat
     1501 tccatcggca gcgcaattcg aggatctgtc caccgatgac accggcagcc gcgtttggtt
     1561 ttcttcgttg ctccggcttc gcgggaccga ccaggagctg tacgacaagc tcttcaccgc
     1621 agatgccaat ggacatgtca cgctcctgct ccagcgaccg cccgacaatc cgtatcatct
     1681 tctgaaccgc cccgaggttt ctggtgacgg tcgcacactc gcctatctcg cgcggtatta
     1741 ctgtccgccg caggtcacgt gcagggtggc cgtcggctcc cacggcgtaa tcactgttcc
     1801 aggccaaccc gatattgagg ttactggcag catccatctg agccgaaatg gcagatacat
     1861 catgcgggac agctatcgtt acttgggtcc gacggaccag gtggagatga tcgacctgca
     1921 gaccggtacg cgccaccaga tccccgtagc gaactttctg cctatcagcg gaggacgcca
     1981 agtcgcttcg aatggatcgg ctctggttta cgcgaacgga ctttggctca tccggttcga
     2041 tggcagcttc cagtttgtgc cgacctggaa cttcggtcca tcatcggatc cgcagcagtg
     2101 gtttcccgcg gcactgagcg ccacaatgga cgactccgcg agcattgtgg tgtttcagac
     2161 gatggctggg acgccgggct tgggcatcgt gaggccggac gatagttcct cactacggac
     2221 cctcgtttcc gctgaagaag gctgcacaaa tccagtgatg tcggcggacg ggaagcgggt
     2281 cctctttctt tcacaagcca acttttccgg cagcaacacg cgcgggctgc agcaggcctg
     2341 gattgtcagt acggattccg gcgccatcca gatggtcacg aatgaccccg caggtattga
     2401 ggaagcgacg ctctccggag atggaaatgc agtgtgggcc agtacgttcg caggccgtct
     2461 cttgtggatc cgggtcgata cctccgtcgt gcgcgagatc attccttgga ccacagcggt
     2521 cgattcggat cctctcaatt tcccgatccg ggctgcacca ggatccctcg cgcagttgac
     2581 cggccgaggc ttgtcttctc aacaggtgaa ttccggtctc cctctggcca cagaactatc
     2641 aggagtgcgg ctactcgccg atggggcacc tctcccgctg ctctttgtct ctccaaagga
     2701 gatcggattt caggttcctt gggagatgcg aggcagacac actctcacgc tggcgcccac
     2761 ccgatcaccc tttgaagatc aactcacaag gcaactgcag gttgtcgata catccgtgga
     2821 gctcctgaac acggcagatg gacagccggc gattgcgcat gatgacttcc atgggttggt
     2881 cacgccgaac gatccggcaa ggcccggtga agtgctgcat ctatacctca ccggcctcgg
     2941 ggctgtcgat tcccccgtcg cgaccggaga cgcggcgccg atggctccgc tctcaaccgt
     3001 aacagaccgg gtatctgttt attgggctgg ttcggtgctg cctgctcctc cgccttcggg
     3061 ccccgaggtt ttattcgccg ggcttgcgcc aggtatggtc ggcatctacc aggtggatat
     3121 ccgcctgccc gcaaacgcac cgctcgcgca attggggatc tcgattcagg tcggggatac
     3181 caacaccgtc acaacttttc cagtgggtgt tccctgatag atcgctgctg agcatccgag
     3241 cagaagtggg taaggaccga acggcaacga aacggtcaga tttcagatct cctcctccga
     3301 aacctgatgt agaatcggtg aggccaaacc atggccctgg aggaagtagt gcgccacacg
     3361 ccgtcttgca tgactcgccg ccggttcgga ttgactctgt gcggagtgcc tctttcctat
     3421 gcgctggcag ccgaggactt tggagcgatg ccgtgggccg gaccggccag cgtcgccaag
     3481 gtgtacctcg ctggcgccac cattcactgg ccgaagcccg acctcgacgt ccagaaggac
     3541 atcgccgaaa tcgaggcgag gcttgccgag ttcgagcgca agcacccgaa cgacgtacgc
     3601 ttcaccggcg gtcagttgtt gcggaaagag gaggacgtcg caccgtgggc acagagtatc
     3661 gcggatgccg acgctgtcct gattctgccg ctcgcccagc cgctcggacc gctggggcag
     3721 gtgttggacg cgatcaaggt ccctgcgctc tacttctcgc gcccgtacgc cgggcactcc
     3781 tgggcatcca tcgcaggcta ccgcaagagc gggaagcgcg tggacgccgt agcctcatcc
     3841 agctacggag accttgacgc ctacgcgggc attttccgaa ccatccacca tctgcggaag
     3901 agccgaatgc tcgtcgtggc aaacagcccc cgaggccggg acgctcaggc caaagcttac
     3961 tcggagtact tcggcacgga cttccgtttc ctcacttacg acgacctcaa tgctgcgttt
     4021 gcggatggcg acgaacgcaa ggcccggcag gcagccgagg agttcacccg cggcgcgctg
     4081 cgtgtcgttg aaccgaagcc caaggaaatc caggacgcgc tccgcttcta cttcggcatt
     4141 gccggcatga tgaagcgcga gaaggccaac gctgtcaccg tggactgctt cggtgggctt
     4201 ctggcgcaca aactgccggc gtatccatgc atcacctggt cgaagctgaa tgaccaggga
     4261 ctgtacggcg tctgcgaagg cgacatcgaa tcgacgatga gccagatgct ggtgacttcc
     4321 tactccggca tgccgggttt cgtctcggat cctgtgatcg atctgagccg caacgagatc
     4381 atccacgcgc actgcgtcgc cgcgacgaag atgaagggca tcggcggctc ctcctcgccg
     4441 tacatcatcc gtaaccacct ggaaacgaat gaaggcgcgg tgttgcaggt gctgatgcca
     4501 tccggcgaga cgatcacctg cggacgcttc gctactccga agaagttcct cgtctccacg
     4561 gccgtcgtta ccggcaacgt ggactccgac cgcggatgcc gcagccagat ccgcacgcag
     4621 gtcaccgatg ctcagaagat gctcgaggcc tacagcgccg gcctgcaccg ggtcatcttt
     4681 tacggcgacc acgtgaagac ggtcgagcgc atgggcagga tgatgggctt cgaagtcgtg
     4741 aaggagatct aggggcagcg gggagccccc ttttgtttcg ccgctccatc atgtatactg
     4801 cgggttaaga gtttaagacc ctctagtgtg aataccggcg cccgccggcg ctagagtctc
     4861 aggggtcgta ttgaatcccg tcgcgccgag gagatccttg cgtccatgaa gagcctgcta
     4921 gaacctccac cagcgagaac cgaagaccag gactttatcg accgtatcat caaagaacag
     4981 ggcgaccgcc cgggcgccct gctcaccgtc ctggagcgtc ttcaagaacg ccacccgaac
     5041 aagtacctgc cgatggagac gctggaatat gtcgccgccc ggaccgacat ccccgtggct
     5101 caggtctgga gcgtcgtgac cttctacgcg ctgttcaacc tcgagcccca aggccagcat
     5161 accgtctgta tctgccgcgg caccgcctgt catacgcgcg gctcgcgcag cctgctcgaa
     5221 cggctccgcc ttgaactggg cgtcaacaaa aaggacgatc agcacgaggc agacaaactt
     5281 tctctaacca cgccggaccg ccgcttcacg ttgcggaccg tcgcctgttt cggacagtgc
     5341 gccctcgccc cggtggtcga gatcgatcac gcgatttacg gtcacatgaa cgagcagact
     5401 ctccaccgtg agctccaggc tttgaatcag gagggcgcaa agtgagacgg atcaccgaca
     5461 tcgccagctt cagcgaagcg aaagctgccg gcatggctaa gctgatgccc ttgcgcccac
     5521 gcatcgccgt gggcatgggc acctgcggaa cgggcaacgg cgccgagtcc gtttaccagg
     5581 cattcgcctc ggccatcgac ttccgcggcc tcgacgccga actgacccgc accggctgct
     5641 tcggtttctg cgcgcaggaa cctctggtga acgtccggct tc
//
LOCUS       DATIFQ010000224         4948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_648930, whole genome shotgun sequence.
ACCESSION   DATIFQ010000224 DATIFQ010000000
VERSION     DATIFQ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648930"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VLE22_10510"
     CDS             complement(<1..464)
                     /locus_tag="VLE22_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HSB14880.1"
                     /translation="MTSARIPVALGAAVVAILGISASPRKAVIIGYLFPRNELIVPSE
                     IAADKLTHINYAFADVRNGRIVEGFARDTENFQQLADLRREHPHLQILISVGGWTWSN
                     NFSDAALTPESRRRFTDSAIEFVRRHDIDGIDIDWEYPGLPGNNNVHRPEDK"
     gene            565..957
                     /locus_tag="VLE22_10515"
     CDS             565..957
                     /locus_tag="VLE22_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14881.1"
                     /translation="MNANPTSGSLFNWTQFIYALHAFSLLTGILGAATVVGAFLTGWP
                     SLIAVVLNYVKRSDVRGTWLESHFRWQIRTFWYGLLWVSLCILFVIMTLGIGILIAWL
                     PLAIVALWFVYRILRGWLALREGRPMPI"
     gene            complement(1304..3043)
                     /locus_tag="VLE22_10520"
     CDS             complement(1304..3043)
                     /locus_tag="VLE22_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB14882.1"
                     /translation="MSRPDSDWTFRFGEFELDISAYELRRGGQRVHLERQPMDLLILL
                     IERNRQLVSRADIVRRLWGPGVFVNVEMGVNTAIRKLRKALQDSRESPAFVETISGKG
                     YRFIAPVNAVFLGRHQEAIESVAVLPFANDSADPDAEYLSDGIAESLINNLSQIRNLR
                     VVARSTVFRFKGRDVDPQKAGNELRVRAVVLGRLLQRDSTLIVRAELMDVATGAQLWG
                     GQHRRKVEDVFALQDDLSKEISEKLRLKLTSNERQRLTRRYTEDAEAYRLYLKGRYHW
                     NKRNAEGLQKAVEYFEQALDKDPSYSLAYAGLADTYAYFAFFNVVAPWEAMPKAKIAA
                     VKALEIDKDLAEAHVALGYVSFTYDGDWAAAGKHFEQALAFNPAYCRAHTFYPLYLSS
                     LGRSEEALEVAKRALDLDPASPAGSHSLAVQLYLAREFDRAIEQAHYTLEMDANFAIS
                     YVVLGEVYLSKGMYRECLSALEKYSTLSRGGATLLALLGYSYARLGERNEALQMIHEL
                     EAATKQGFAPALFVALVYAGLEDKDQAFIWLEKASEQRFTRLAYLKVDALWDPVRTDP
                     RFGDLLRRVGIPA"
     gene            3250..3759
                     /locus_tag="VLE22_10525"
     CDS             3250..3759
                     /locus_tag="VLE22_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14883.1"
                     /translation="MMSLNSERFAVSDRTAALVLASMLLLGSGRTARAQVEDPGSRNR
                     LEGTWHLVVTVRDCQTGEEMRHFPALAAFSKGGTLAVTTAGQSPSLSTTGLGVWRHTE
                     GQSYSAVFETFVFSPAGVWIQKHRITRVIDIVDDGDTFMDTVALEVFDANDNLIVRGC
                     GTSVANRFK"
     gene            complement(4035..4712)
                     /locus_tag="VLE22_10530"
     CDS             complement(4035..4712)
                     /locus_tag="VLE22_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14884.1"
                     /translation="MTRDRPNRGHVAVVFGSTGTAGMGVVHACLADPGVSEVRAITRR
                     PLSISHARLHQVQCSDFVNLEGLAQQFEAVDCCLFCLGTSVRNVNGEDEYREIHETYA
                     LAAARTLLAKSPDASFVYLSGAGAKRTSRMMWARVKAEAEDRLAELKLTRHVNVRPRA
                     ILPMHPTGVNRWLLAPLLNLIPALGIKSVDLGRAMLRVGLDGNWHGSRTLENKDLKAL
                     LGHEQHR"
BASE COUNT          988 a         1472 c         1466 g         1022 t
ORIGIN      
        1 tccttgtcct ccggacgatg cacgttattg ttgccgggta gaccggggta ctcccagtcg
       61 atgtcgatgc cgtcgatatc gtgccgccgt acgaactcta tggcgctgtc ggtgaaacgt
      121 cggcggctct caggcgtgag agccgcgtcg gagaagttgt tggaccaggt ccagccgccg
      181 accgagatga ggatctgcag gtgtggatgc tcgcgccgca gatccgcgag ctgctggaaa
      241 ttctccgtgt cgcgggcaaa gccttcgaca atgcggccgt tccggacgtc ggcgaacgcg
      301 tagttgatgt gcgtcagctt gtcggccgcg atttcggacg gcacgatcag ctcgttgcgt
      361 ggaaacaagt agccgatgat caccgctttc ctcggcgagg cggagatgcc gagaatggcc
      421 acgaccgctg cgcccagcgc aaccgggata cgtgcggatg tcatcgatcc tcctgggact
      481 acggattacc tacaactgta gctgagtccg ccgccccgcg ctccccgtat tgacgcggcg
      541 ccggaggcga ggtcgaatag cgcaatgaat gcgaatccca cttccggctc cctcttcaac
      601 tggacgcagt tcatctacgc actgcatgcg tttagcctgc tcacgggaat ccttggcgcc
      661 gccacggtgg tcggcgcctt cctgaccgga tggccgtcgc tcatcgccgt ggtcctgaac
      721 tacgtgaagc gcagcgacgt gcgtggcacc tggctggagt cgcatttccg atggcagatc
      781 cgcaccttct ggtacgggct gctgtgggtc agcctgtgca tcctcttcgt gatcatgacg
      841 ctcgggatcg gaattctgat cgcgtggctg ccgctcgcca tcgtggcgct ctggttcgtg
      901 tatcgcatcc tccgcggctg gctggcgttg cgggagggcc ggcccatgcc gatttgaagc
      961 ggggtgaggg cgcacgcacg tgttcgaccg gatcgccagc caggcgaaac ctgaaggtgc
     1021 ttacagacga gatcttgacg cattaagctg gcggaggcct cgtccacctg cggtgggcag
     1081 aacgcccggc gtttgagcga acgcctcggc gcgtccccgg tttaagccct cattcggttc
     1141 gagaacgacc ctcggctccg cgtcgagctc aaaacgcatt cagcgtgacg cgcgtccttg
     1201 gcaggtaagg tttgaaaaat gcctatttcc tcaatattgt cggcacgcag ccggggcttt
     1261 cggcggtcag cctctggatc gccgacgact cggccaaacg agcttacgcc gggatgccga
     1321 cgcgtcgaag caggtcgccg aaccggggat cggtgcgaac ggggtcccag agtgcgtcta
     1381 ctttcaggta agcgaggcga gtgaaccgct gctcggatgc tttttctagc caaatgaagg
     1441 cctgatcttt atcttccagc ccggcgtaaa ccagcgcaac gaaaagggca ggtgcaaaac
     1501 cctgcttcgt agccgcctca agctcatgga tcatctgtag tgcctcgttt cgctcgccta
     1561 atcgagcata cgagtatcca agaagggcga gaagggtggc gcctccacgg cttagcgtgg
     1621 aatatttttc aagcgcggac agacattctc gatacattcc tttcgagaga tacacctctc
     1681 ccagcaccac gtatgaaatc gcaaaattcg catccatctc cagcgtgtaa tgggcctgct
     1741 caatagctcg gtcgaactct cgtgcaaggt acaactgtac cgccaagcta tggctgccag
     1801 caggggaagc aggatcgaga tctaaggcgc gtttcgctac ctccagggct tcctctgatc
     1861 gtccgagaga actgagatag agaggataga acgtgtgagc tctgcaatac gctggattga
     1921 atgcgagggc ttgctcaaaa tgctttccgg cggccgccca gtccccatcg taggtgaaac
     1981 tgacgtatcc gagtgcaacg tgagcttccg cgaggtcttt gtctatctcc agtgccttca
     2041 ccgcggcgat ctttgctttc ggcatggcct cccacggagc aaccacattg aaaaacgcga
     2101 aataggcgta ggtgtcagcc aaacctgcat aagccaggga ataggatggg tctttgtcga
     2161 gagcctgctc aaagtattca acggctttct gcaaaccttc tgcgttccgt ttgttccagt
     2221 gataccgtcc cttcagataa agccgatacg cttcggcgtc ttcggtgtag cgtcttgtca
     2281 ggcgttgcct ttcattgctg gtcaacttca gccgcaactt ctcagaaatt tctttggaaa
     2341 gatcgtcttg gagggcaaaa acatcctcaa ccttgcgtct atgttggcca ccccagagct
     2401 gcgcacccgt tgcaacatcc atcagttccg cccgaacgat cagcgtactg tcgcgctgca
     2461 gcaatctccc caagactacg gcgcgaacgc gtagctcgtt tcctgccttt tggggatcga
     2521 cgtcccttcc tttaaagcgg aacaccgtgc tgcgtgccac cacacgcagg ttccggattt
     2581 gcgagagatt gttgatcagg ctttcggcga tgccgtcgct caagtattcc gcgtcgggat
     2641 cggcgctgtc attggcgaag gggagcaccg ctaccgactc gatggcctcc tggtggcgcc
     2701 ccaaaaagac cgcgttgacg ggagcgataa agcggtatcc cttgcccgaa atcgtttcaa
     2761 cgaacgccgg cgactctcgc gaatcctgca gagctttccg cagcttacgg atcgctgtat
     2821 tgactcccat ttcgacgttc acgaagacac ccggacccca caaccgccgt acgatgtcgg
     2881 cccgggaaac aagctgccga ttgcgttcta ttaaaaggat gaggaggtcc ataggttggc
     2941 gctcaaggtg gaccctttgc ccgccccggc gcagctcgta ggccgatata tcgagctcga
     3001 actcaccaaa tcgaaacgtc cagtctgaat caggacgcga cataggctcc tgcgccccac
     3061 gatttcctaa gacacctgga acaggtgctc tgggcccggg tattggcgaa cggccgatga
     3121 tatccgccgc ttcagaaata ctccagatta aatacaggcg gtctccatgg tcatcgatgc
     3181 aggccaatgt cactatttcg cagagtggaa ccatctcagc ggaactcaga acaagaagga
     3241 gaactcccga tgatgtcgct taattccgag cgctttgcgg tttccgatag gacggcggca
     3301 ttggttctgg ccagcatgct gcttctgggg tcgggaagaa ccgccagggc tcaggtggag
     3361 gaccctgggt ctcgaaaccg ccttgaaggg acatggcacc tggtggtcac ggtgcgcgat
     3421 tgtcagacag gcgaggagat gcggcatttc ccggccctgg cggccttctc gaaaggaggt
     3481 acgctagcag tgacaaccgc aggccagtcc ccctcgctaa gcactaccgg tttgggcgtg
     3541 tggcggcaca ctgagggcca gagttacagc gcggtcttcg agaccttcgt ttttagcccc
     3601 gcgggagtgt ggatacagaa acacaggatc acgcgggtca tcgatatcgt tgacgacggc
     3661 gacacgttca tggatacagt tgctcttgaa gtcttcgacg ctaacgacaa cctgattgtg
     3721 aggggctgcg gcaccagcgt cgcgaaccgc ttcaaatgaa tggcggaaag accgtcggag
     3781 cggaagccaa tgagggtcgg taacggggca aggtggttcg gccatccgat cccatgctgt
     3841 tcctgcccga tgcaggatgg ctcgtcggga ccgtgcaagc cgtcgcgcaa cgccaccggg
     3901 tgcccctact agagagagac caggacgcaa tcgtggcaat cgtcggcagg ccactcgtgc
     3961 tcgtcggata cgacgaagac ggtagggctg aactccaccg gtggtgccgt gcgggttcgg
     4021 atggaccgaa ccgcctaccg atgctgctca tgtccgagaa gcgctttgag gtccttgttc
     4081 tcgagcgtgc ggctcccgtg ccagtttccg tccaagccca cccgcagcat cgcccggcca
     4141 aggtcaacgg atttaatccc cagcgcggga atcaggttca gcagcggcgc aagcagccag
     4201 cggtttacgc ccgtcggatg catcgggaga atggcacgcg gacgcacgtt gacgtgacgg
     4261 gtcagcttca actccgccaa tcgatcctcc gcttctgcct tcactcgcgc ccacatcatt
     4321 cgggaggtgc gcttcgcgcc cgcgccagac agataaacaa agctcgcgtc agggctcttt
     4381 gcgagcagtg tgcgagcggc agcgagggcg taggtctcgt ggatctcgcg gtactcatcc
     4441 tccccgttca cgttcctcac cgacgtgcca aggcaaaaca ggcagcagtc gaccgcttcg
     4501 aactgttgcg caagaccttc gagattgacg aagtccgagc actggacttg gtggagcctg
     4561 gcgtgcgata tcgataatgg gcgacgcgtg attgcccgca cctccgacac acctgggtca
     4621 gcaaggcatg catggaccac acccattccg gcggtgccgg tcgagccgaa gaccaccgcg
     4681 acatgtcccc ggtttggtcg atctcgagtc atcgctgcgg taccttacaa gaagatcatg
     4741 gcttgtattg tctcagacct cctgatgtcg ctcgatcact tatggctttt tcgcagagca
     4801 ggtgcattcg tatcgcgcca gccgatcatc cgccgccgtc ctacgtgctg cactcatcgc
     4861 aggggcgctt cagcaggagg gggacgcaga ttcgtttcgc cgagctcagc ctccctgggc
     4921 ctgcccatgg aagaaggcgg ccatgtcg
//
LOCUS       DATIFQ010000225         7916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_265018, whole genome shotgun sequence.
ACCESSION   DATIFQ010000225 DATIFQ010000000
VERSION     DATIFQ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265018"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VLE22_10535"
     CDS             complement(<1..971)
                     /locus_tag="VLE22_10535"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14885.1"
                     /translation="MKRVGSPVPSPDGKWVVFSVTEPSYDPTKQVSDLWIVPADGSAK
                     PRRLTFTKGSESGVAWSPDSRRIAFSAKREGDDVAQVYVLDLGGGEAIRMTSLETGAE
                     SPRWSPDGKSILFNSLVYPADGVDRKARKYNALVYNDFPIRHWDRWLDERHAHPFVQA
                     AEPGAKPRDLFSGTKVAFQAGFGAELGNTGEELDAIWTPDGASIVFAATLNRNAAAYD
                     EVVFHLFRTKVDGGEPEKLTGGEGSYGKPAFSPDGKNLYAVFNPSTDKIYNLNRIARF
                     VWPAMGKLAVITASFDRSADSFEVSPDGRTVYVLAEEHGHDKIFAVPA"
     gene            complement(1081..1695)
                     /locus_tag="VLE22_10540"
     CDS             complement(1081..1695)
                     /locus_tag="VLE22_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HSB14886.1"
                     /translation="MFGFLFSGLGLGLAAGLSPGPLLALLVAQTVRHGTREGLKVAVT
                     PLLTDLPVIAVSLLVLARLSSREPLLGAVAMAGAAYLIWLAWGSLRFRSGDWRPSDVS
                     PGSVRKALVTNLFNPHVYLFWIAVGSTMVLNAWTQHPSWAVAFLAGFYSCLVGSKLML
                     AVLIGRSRQFLSGRGYAVAIRVSGVFLAGMAVWLVRDGVRLLFG"
     gene            complement(1772..2290)
                     /locus_tag="VLE22_10545"
     CDS             complement(1772..2290)
                     /locus_tag="VLE22_10545"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HSB14887.1"
                     /translation="MRLKFRYPGHKEFEVISEQGSGAIRKRVFRKMLDSEIEASRDDL
                     RDTTQITPRNYTFRLVGSEELAGRKSFVLEVTPKTKNKYLFKGKVWVDAEDYGIARIE
                     GTPAQNPSVWVRKTSFVHQYGKMGPFWLAVSTTSSTDVWVVGRTDLLIEYSGYRINEQ
                     SGGKPSETGSNE"
     gene            2667..4046
                     /locus_tag="VLE22_10550"
     CDS             2667..4046
                     /locus_tag="VLE22_10550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB14888.1"
                     /translation="MRAILADLKASGCVIAKDTVAGGYGSRFVPFSKVTSVYCYFKQR
                     RLDLPSIQMGYLAAILSGHGHDVVFTRDAIPAGDVALVLSSLVDYRRENEWADEARHR
                     GIRVGFVGLACSKMPELFLDHADFLIMGEPEAAASRLACGERLTGQIPSPPVADLDSL
                     PFPKWELPHEAGGKWRHARRRRFPVLASRGCTEFCTYCPHRILAGYRSRSVRNVADEL
                     EQLSERYPRPHIVFRDPLFTHDRDRCLSLCDEILSRGLSLSFDCETRLDGLDDDLLVV
                     LRRAGMRAVSFGVETVAPETLRRVARRPIPEPHQRAVVDACHRLDIMTVGYYVFGFLQ
                     DDWSTISATIEYSIALGTTFAQFKVLTPYPGTPMWKQFAPLVFEGDWQQFNGYTPTFH
                     HPNVTAEDLQFLLGAAYARFYARPGWLVNYWNLREFLSNALLHRMDRKVLQWHAEKEI
                     SLVSRAAEC"
     gene            4040..5317
                     /locus_tag="VLE22_10555"
     CDS             4040..5317
                     /locus_tag="VLE22_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSB14889.1"
                     /translation="MLTAISRYGARVLPDTQQLIADCVGWGELVEGPAIEEFEEAFSQ
                     RHENRSAVAASYGRMAFYYILKALNLPAGSEVIFPALTFWVVPEIARVLGLRPVFVDV
                     DRKTFNMNPSAVDRAVTAKTRAIVPTHLYGLPCDMDEILDIATKHNLAVIEDCAHSLG
                     ALYRGRPVGTFGEAAFFSFQTLKPLNTYGGGMAVVRDASLASAVRGLAHSEPLPTEAE
                     VRKKLNIGRVQRILTRPSVFRWTLFPILWAASFVRARPDVYLWEAIRPLDPLPPDYRR
                     RYSNVQAVIGLEALRHLAGWTAATRRNAELLAQTMTGVPGVQLPVCPADRTHVFYQYA
                     LRVGDRDRVVRSCIRRGLDIETLHVDVCTRLPMFQPFWSPAPEADYAAEAVQLPVYAS
                     LSENQVRWVSRIVRGAVSGNSPAPTTVPQSARD"
     gene            complement(5611..7908)
                     /locus_tag="VLE22_10560"
     CDS             complement(5611..7908)
                     /locus_tag="VLE22_10560"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HSB14890.1"
                     /translation="MSHDLTQVPHVGRFIHGRSHRTVTVHLDPWIKRARRSLSVIRRY
                     ESPSTPPPLILNRHCGECEFQERCRKLAVDHDDLSLLANLSDKEWRKQHDKGIFTVTQ
                     LSFTFRPRRRVSPLPVHHQYALKALAIRTKKIHVLGSAPPDSAPVTPVYIDVEGDPDR
                     NFYYLIGLRLSRDGSPIQHSFWADDPASERTIWTDCLRILSSIDNPTLVHYGSYETAF
                     FKHMRDRYSDCVPAFVDKLIAEAINLVSIICAHVYFPTYSNSLKEIGAYVGFRWSAAS
                     ASGLNTLIWRSQFEQSQNAALKQKLITYNAEDCQALQMVAQTVWRVLDQTQAAQNATV
                     DVSTLKREFPQRFGSVDFLLPEFKAINEAAYWDYQRNRVYIRTEGSRSKLYRRKGAKK
                     SQVRPNRVVDVEDLRPDRCPRCEATLIYKWGTLSQTVYDLRFTNTGIKRWVVRFVFQR
                     FICWRCKRPFQFYAQKPKYGTALFAYVLYQLFEMGVSQNAIAASLSRLFGLPFSRGGV
                     NHLKEAAARKYRATYEDILRRIASGRVIHADETKAKVEGRDGYVWAFTSLEEVAFVYR
                     ATREAEFVHEMLGTFRGVLVSDFFSGYDSIPCAQQKCLIHLMRDINEDLRKHPFNQEM
                     RQIAEAFACLLRPMVATVDRFGLKARHLRKHRPAVEKFFYALEKSDYQTDVAVAHRRR
                     FEKNRNKLFTFLDCDGVPWNNNNAEHAIKAFARLRRSIGGTSTVKGMEDYLVLLSISE
                     TCKTKGADTLDLFLADDAGSFAART"
BASE COUNT         1630 a         2303 c         2302 g         1681 t
ORIGIN      
        1 gcggggacgg cgaagatttt gtcgtgcccg tgctcctcgg cgaggacata aacggtccgg
       61 ccgtccggcg aaacctcgaa agagtcggcg gagcggtcaa aactcgctgt gatcacggcg
      121 agtttgccca tcgccggcca cacaaaccga gcgatccggt ttagattgta tatcttatcc
      181 gtgcttggat tgaagacggc gtagaggttt ttgccgtccg ggctgaaggc gggcttaccg
      241 taactaccct cgccgccggt cagtttctcg ggctcaccgc catccacttt ggtccggaaa
      301 aggtggaaca cgacctcatc gtaggccgcg gcgttccggt tcaaggtcgc ggcgaaaacg
      361 atcgatgcgc cgtccggcgt ccagattgcg tccaattcct cgccggtatt gcccaattcg
      421 gcgccgaaac cggcctgaaa tgctactttt gtgcccgaaa acaggtcccg cggtttggcc
      481 cccggctcgg cggcctgaac gaatggatgg gcgtgccgtt cgtcgagcca gcggtcccag
      541 tgccggattg ggaagtcatt gtaaacaagt gcgttatact tgcgggcctt tcggtcaacg
      601 ccatccgctg ggtacaccag gctgttgaaa agaattgact tgccgtcggg gctccagcgc
      661 ggcgactcgg cgccggtttc gagcgaagtc atccgaatcg cttcgcctcc accgaggtcc
      721 agaacgtaaa cctgcgcaac gtcgtcgcct tcgcgctttg ccgaaaacgc gatcctgcgg
      781 ctgtccgggc tccaggcgac gccgctttct gagcctttcg tgaaagtgag gcggcgtggt
      841 ttggcgctgc cgtcggccgg aacgatccag aggtcggaaa cttgtttggt tgggtcgtaa
      901 gacggctcgg tcacggagaa gactacccat ttcccgtccg ggcttggaac gggagacccg
      961 acgcgcttca tgagccacat cgtctcgtgt gagatgggcg ttttggcgtc aaaatgtgca
     1021 caaaacagcg caaaaacgag caaaaaacga ccacaaaatg ccatagtttt cacgatatca
     1081 tcacccgaaa agcagccgga ccccgtctcg aacgagccag acggccatcc cggcgaggaa
     1141 cactcctgac actcgaatgg ccacggcgta cccccttccg ctgaggaact gccgcgaccg
     1201 gccgatcagc accgccagca tgagcttcga accaaccaga cagctataga atccggctag
     1261 aaacgccacc gcccaggacg ggtgctgcgt ccaggcgttg agcaccatcg tgctgcccac
     1321 tgcgatccag aacaggtaga cgtgcgggtt gaacaggttc gtcacgaggg cctttcgaac
     1381 ggacccgggc gatacgtccg aaggccgcca gtcgccggag cggaaccgca gagatcccca
     1441 cgccagccag atcaggtacg cggcgccggc catcgcgacg gcgcccagca gcggctcacg
     1501 gctcgagagt cgcgcgagca caagcagaga taccgctatg actggcaggt ccgtgagcag
     1561 gggcgtgacc gcgaccttga gcccttcgcg cgtgccgtgc cgcacagtct gagcgacaag
     1621 cagagccaag agcggcccag gcgagagacc cgcggcgagg cccaggccaa ggcccgaaaa
     1681 cagaaagccg aacaaaggaa tagtgtagcg ccgttttaca cgcgcgcgat ttcttggcgg
     1741 gcggacgcat cggcagcgga caagcgagat gtcactcgtt agaccccgtt tcggacggct
     1801 ttccgcctga ctgttcatta atccggtagc cggaatactc gatgagcaag tccgtccggc
     1861 cgaccaccca gacatccgta gaggatgtgg tggagaccgc cagccagaag gggcccattt
     1921 ttccgtactg atggacaaag gatgtcttac ggacccagac cgatgggttc tgcgctggcg
     1981 ttccttcgat ccgggcgata ccgtagtctt cggcgtctac ccaaaccttt cccttgaaca
     2041 ggtacttgtt cttggtcttt ggtgttactt cgagcacaaa cgatttgcgc ccggcgagtt
     2101 cttctgaacc gaccaaccgg aacgtgtagt tcctgggcgt gatctgggtc gtgtcccgca
     2161 gatcgtctcg tgatgcctct atttcggaat cgagcatctt tcggaaaacc cgtttgcgga
     2221 tcgcccccga accttgctcg ctgattactt caaactcctt gtggccgggg tatcgaaatt
     2281 tgagccgcac ggtcatctcg gctctctggc caaaccgttt gttttccaag acgtaccggc
     2341 gaacagagct ataatccctg agccgcggag tcaggagtcc atccaactcc atcatccgcg
     2401 ctacgatttg ttccgtgggg gagaccgccg cctcaactgg agagccccag agggcagcag
     2461 ccaacgcgat tggcagcacc tgcaaccgag cgtacaccct ctaggctcac accgccaaat
     2521 accccccgta aaatcggtcc ggagcttagc gtcaatgcgg ggtatccggt gaccgtaccg
     2581 gatcttacgc tggtctagta tcacatctcc gactggcaca ccggaggctc atctgtacgg
     2641 ccgtaggggt ggattgggca tagcgcatgc gagcgattct ggcggatctg aaggcctcag
     2701 ggtgtgtgat tgcgaaagat acggtggcag ggggttacgg atcgcggttc gttcccttct
     2761 ccaaagtgac cagcgtctac tgctacttca aacagagacg actcgacctg cccagcatcc
     2821 aaatgggcta tctggccgcg attctttccg gacacggaca cgatgtggtc tttacccgag
     2881 acgccatccc ggcgggcgat gtggcgttgg tcctctcctc cctggtcgac taccggcgcg
     2941 agaatgagtg ggcggatgaa gcacggcacc gtgggatccg ggtcggcttc gtaggccttg
     3001 cctgctcgaa aatgccggaa ctattcctgg atcatgccga ctttctgatc atgggagagc
     3061 cggaagccgc ggcgtcccgg ttggcttgcg gcgagcgctt gaccgggcag atccccagtc
     3121 ccccggtggc tgacctagat tccttgccat tcccgaaatg ggaactgccg catgaggctg
     3181 gaggaaagtg gcgccatgcg cgaaggcggc gttttccggt cctggcaagc cggggttgca
     3241 cggaattttg cacctactgc ccgcatcgga tcctggccgg ctaccgctca cgctccgtgc
     3301 gtaacgttgc cgacgagcta gagcagctta gcgagcgcta tcccaggcca cacatcgtgt
     3361 tccgggaccc tcttttcacc cacgatcgag atcgctgcct cagcttgtgc gacgaaatcc
     3421 tctcacgcgg cctcagcctt agtttcgact gcgaaacccg cctggatggg ctggatgatg
     3481 acttgctcgt ggtgctgcgg cgagctggaa tgagggcagt aagtttcgga gtcgagacgg
     3541 tcgctcccga aactcttcgc cgggtcgcga ggagacccat acccgaaccg catcaaaggg
     3601 cggtcgttga tgcctgtcac cgtttggaca tcatgacggt gggctactac gttttcggat
     3661 tcctgcagga cgactggagc acgatatccg caacgatcga gtactcgatc gctctcggca
     3721 cgacttttgc gcaattcaag gtgctcacac cctatccggg taccccgatg tggaagcagt
     3781 tcgcgccgtt ggtctttgaa ggagactggc agcagttcaa cgggtacact ccaaccttcc
     3841 atcacccgaa tgtcacggcc gaggatctgc aattccttct tggggctgct tatgcgcgat
     3901 tctacgcgag accgggatgg cttgtgaact actggaatct gcgtgagttc ctctccaacg
     3961 ccttgctgca caggatggac cggaaggtgc ttcagtggca cgccgagaaa gaaatctcgt
     4021 tggtatcgag ggccgcggaa tgctgacggc aatttccaga tacggggcca gagtgctgcc
     4081 cgacacccag cagttgatcg ccgattgcgt gggttggggg gaacttgtcg aagggccagc
     4141 gattgaggag ttcgaagaag ctttttcgca acggcacgaa aatcggtctg cggtcgccgc
     4201 gtcctatggg cggatggcgt tctactacat cttgaaggcg ctcaacctac ccgcgggatc
     4261 ggaggtcatc tttcctgcct tgactttctg ggtggtcccc gagatcgcac gagtcctggg
     4321 tctaaggccc gttttcgtag acgtcgatcg gaaaacgttc aacatgaatc ccagtgctgt
     4381 cgatcgtgct gtcactgcga agactcgggc aatcgttccg acgcacctgt acgggcttcc
     4441 gtgcgatatg gacgagatcc tcgatatcgc gacgaagcat aatcttgcgg taatcgagga
     4501 ctgcgcgcat tcgctcggag cgttatatcg cggcagacct gtcggcacgt ttggcgaggc
     4561 ggccttcttc agctttcaaa cgctgaaacc gctgaacacg tacgggggcg ggatggccgt
     4621 agtccgagac gcctccctcg caagcgctgt ccgcgggttg gcgcattccg agccccttcc
     4681 caccgaggcc gaagttcgca agaaactcaa catcgggagg gtgcagcgca ttctgaccag
     4741 accctcggtg tttcgctgga cgctctttcc aattctctgg gcggcatcgt ttgtgcgagc
     4801 tcgacccgac gtctatctct gggaagccat tcgtccgctc gatccgcttc cgcctgacta
     4861 ccgccgccgt tatagcaatg tccaagctgt catcggcctc gaggcgctgc gccacctcgc
     4921 cggctggacc gcggcgacac gccggaatgc ggaactgctc gcacaaacga tgaccggggt
     4981 tccaggtgtt caactacccg tctgccccgc cgaccgaacg cacgtcttct accagtacgc
     5041 tctgcgcgtg ggcgaccgcg atcgggtggt gaggtcgtgt attcgccgcg gcctggacat
     5101 cgagacgttg cacgttgacg tttgcacccg gctgccgatg tttcagcctt tctggtcacc
     5161 ggccccggaa gccgactatg cagccgaagc cgtgcaatta cccgtgtacg cgtctctctc
     5221 agaaaaccag gtcagatggg tttcccgcat cgtaaggggc gccgtctccg ggaactcccc
     5281 agcgccgaca acggtgcctc agtccgcgcg ggactgaacg gctcgattca cccatgtggc
     5341 tccgccgcgc ttatcccgct ggttttgccg ggaagttcac cacgaaggca caaagagtaa
     5401 ggctgcgggg agagctatac gagcgggaaa actgctacat ttcggacatt cacatccgat
     5461 attgctgcac ctatgccccc tcttctgagg ggcggtgcag gcaggtgcaa gcgaggcgtc
     5521 cgctcgcgcg cgctttgggg tcctcccgac gggctgtgcg cgcaggacta gggaacagca
     5581 cgaagggagg ggcggtgcga attgggtcgg ttaggtacgc gccgcgaacg atccggcatc
     5641 gtcagccaaa aacaagtcga gcgtgtcagc gcctttcgtc ttgcacgtct ctgaaatact
     5701 caacaaaaca agatagtctt ccatgccttt gacagtgctg gtaccgccga tgctacggcg
     5761 tagcctcgcg aaagctttga tcgcgtgctc ggcgttgttg ttgttccatg gtacgccgtc
     5821 acaatctaag aacgtgaaca acttgtttct attcttttca aaccgtctcc tatgtgccac
     5881 cgcaacgtcg gtctgataat cactcttctc cagagcgtag aagaacttct caactgctgg
     5941 ccgatgtttt cggagatgcc gcgccttcaa tccgaagcgg tccactgttg cgaccatcgg
     6001 ccgaagcaag caggcgaacg cttccgctat ctgcctcatc tcctgattaa atggatgctt
     6061 cctaaggtct tcatttatat ccctcatgag gtggatcagg cacttctgct gggcgcatgg
     6121 aatagagtcg tatccagaaa agaaatctga aacgaggaca cccctgaacg ttcctagcat
     6181 ttcgtgcacg aattcagcct ctcgtgtggc ccggtaaaca aaagcaacct cttccaggct
     6241 ggtgaacgcc catacgtagc cgtcccttcc ctcgacttta gcttttgttt cgtctgcgtg
     6301 gatgactcta ccgctcgcga tccggcgcaa gatgtcttca taggttgctc gatatttcct
     6361 cgcagcggcc tccttcagat gattaacgcc accacgcgag aacggcaaac caaacagccg
     6421 cgatagactg gctgcaatgg cgttctgtga cacgcccatt tcaaagagct gatagagaac
     6481 gtacgcaaaa agagcagtgc cgtacttggg cttctgcgcg taaaattgaa atggtcgctt
     6541 gcaccgccaa cagatgaatc gttgaaagac gaatcgaacg acccagcgct tgatgcccgt
     6601 gttggtgaac ctaaggtcat acacggtctg gctcagggta ccccacttgt aaatcaaagt
     6661 ggcttcgcag cgaggacaac gatcgggtcg aaggtcttca acatccacca ctctattcgg
     6721 gcgaacttgg ctctttttag cacccttccg gcggtacagc ttcgaacgac tgccctcggt
     6781 gcgaatgtag acgcggtttc gttggtaatc ccaataggcg gcttcattga tcgccttgaa
     6841 ctcgggcaga agaaaatcca cactgccgaa acgctgcggg aactcccgct tgagagtgct
     6901 cacgtccact gtcgcgttct gggccgcttg agtttggtcc agcactctcc acactgtttg
     6961 cgcaaccatc tgaagggcct ggcagtcttc ggcattataa gtgatcagtt tctgtttcaa
     7021 agctgcgttt tgggattgct cgaactgaga cctccaaatc agggtgttta ggcctgatgc
     7081 tgaggcagca gaccatcgga atccaacgta ggctccaatt tctttaaggc tgttggagta
     7141 tgttggaaaa tatacgtgtg cacagatgat cgacacaagg ttaatcgctt ccgcaatcag
     7201 cttgtcaacg aaggccggga cgcaatcgct atagcgatct cgcatgtgct tgaaaaaagc
     7261 agtctcatag ctgccataat gcaccagcgt tgggttgtcg atggaggaca ggatgcgaag
     7321 gcagtcggtc cagatcgttc tttcagatgc ggggtcatcc gcccagaatg agtgctgtat
     7381 gggcgagccg tcgcgggata aacgcagccc gatgaggtag tagaagttcc tgtcgggatc
     7441 gccctcgaca tcaatgtaca caggcgtgac tggcgcgcta tctggtggtg cggaacctaa
     7501 gacgtggatc ttctttgttc gaatggcgag tgcctttagc gcatattggt gatggactgg
     7561 tagtggggag acgcgtcgtc gaggtcgaaa agtaaaggag agctgcgtga ctgtgaagat
     7621 ccccttgtcg tgctgtttcc tccattcttt atcgctcaag ttggcgagga gactaaggtc
     7681 gtcatggtca actgcgagtt tccgacatcg ctcctggaac tcacactcac cgcaatgcct
     7741 gttcagaatt aagggaggag gtgtgctggg actctcgtac cttcggatga ccgataatga
     7801 gcgccgagcc ctcttgatcc atgggtcaag gtgaaccgtc accgtccgat ggcttctgcc
     7861 gtgaataaat ctaccgacgt ggggcacctg agtaagatcg tgtgacacag ccaaag
//
LOCUS       DATIFQ010000226         4746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1223921, whole genome shotgun sequence.
ACCESSION   DATIFQ010000226 DATIFQ010000000
VERSION     DATIFQ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223921"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VLE22_10565"
     CDS             complement(<1..1537)
                     /locus_tag="VLE22_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB14891.1"
                     /translation="MALYWGQRIVFHRLIVLFLLLAAVAAAGWVRLESPHFELLTNAG
                     EATGRTLLDRLEEIHGVFRSLPGNRPSPPVPTRVLLFRSESDFRPFRLSETTTGYFQA
                     GPERNYIVMRDSGRGNSRIACHEYVHLVLNHSSARLPQWLEEGVAEFYSTLDRDGDRL
                     TVGRPIPEHVRTLSKGGLLGADALASITQKSPHYNDPTKTHIFYAQSWALVHMLNLSP
                     EYRDKMADFVSLIEEGVPSRAAFERAFGKPLQQALQDLEAWLRQGIGTVAVTGIDAPV
                     SVENFALRDLSEGDANLAQAELLVQIGRTETAERIYRRIEREDPRSPDAQTGLGALAL
                     ERSRYKVAREHLLRAMELGANDARTHFEYAMLLRETGASSDEVMASLKKAITAAPSFA
                     EAHFVLGMMLSATEDYAEAANHLARAASILPRQAYFWHALALAYQHLGQLEEARRAAY
                     RAAQAAVTEQEIEMARAAIRLADQPAAADKTRRPPVSTPDSWKEKRGDTRIEGTLRQI
                     DCLG"
     gene            1749..2336
                     /locus_tag="VLE22_10570"
     CDS             1749..2336
                     /locus_tag="VLE22_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HSB14892.1"
                     /translation="MRASQVPHAAELGTLRLKFGEGVTGWVAEHNSVVALGSNASADP
                     RFKRLAGMVEDPYEAFLSVPLVSGGEVTGVLNVHHKEPRAHAAAEITLLAFIGEQMGG
                     AIAKSRLAEENARLLEETLEMKRQLEIRKLVERAKGLLQTRYGISEEEAYLRMRNESR
                     RLRRPMQDLGEAVILAEEMARKAGDQDANGGQPPG"
     gene            4447..>4746
                     /locus_tag="VLE22_10575"
     CDS             4447..>4746
                     /locus_tag="VLE22_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14893.1"
                     /translation="MPPFRPLFANPHLLTIAANFWPRRLDEVRFPVEDTLFETEPGVR
                     VLIRTQRPEGASRGEIIFVHGLEGSSEGGYMRSAAQAALAAGFVAHRYNIRSCGGT"
BASE COUNT          920 a         1386 c         1507 g          933 t
ORIGIN      
        1 aaccgaggca gtcgatctgc cggagcgtgc cctcgatgcg agtgtcgccc cgcttttcct
       61 tccaactgtc cggcgtgctg acgggagggc gtctcgtctt gtccgcggcc gccggttggt
      121 ccgccagtcg gatggctgca cgggccattt cgatctcttg ttccgtcacg gctgcctgcg
      181 cagcccggta ggcggcccgc cttgcctcct ccagttggcc aaggtgctgg taggcgagcg
      241 ccagcgcatg ccagaagtat gcctgccggg gaaggatcga cgcggcccgc gcaaggtgat
      301 ttgccgcttc ggcatagtct tcggtggcgg acagcatcat tcccagaacg aaatgagcct
      361 ccgcgaagct gggggccgcg gtgatcgcct tcttcaagga ggccatgacc tcgtcgctgc
      421 ttgcgccggt ctccctcaga agcatggcgt actcaaaatg cgtgcgcgcg tcgttggcgc
      481 cgagttccat cgcccgcaac aggtgctcac gcgccacttt gtaacggctg cgctcgagtg
      541 ccagcgctcc gagcccggtc tgggcgtccg gacttcgcgg gtcttcccgt tcaatgcggc
      601 ggtagatcct ttctgcggtc tccgtccgtc cgatctggac cagcagttcc gcttgtgcga
      661 ggtttgcgtc gccctcggag aggtcacgaa gagcgaaatt ttcgacggaa acaggcgcat
      721 cgatgccggt aacggcgacc gtcccgatcc cctggcgcag ccacgcttcg aggtcttgta
      781 aggcctgctg caaaggtttt ccgaaggcgc gctcgaatgc ggctctagat ggcacaccct
      841 cctcgatcag cgagacgaaa tcggccatct tgtcccggta ttccggcgaa aggtttagca
      901 tgtgcaccaa tgcccagctt tgggcataga agatgtgagt cttggtggga tcgttgtaat
      961 gtggcgattt ttgcgtgatc gacgcaaggg catccgcacc gagaagtccc ccttttgaca
     1021 gtgtgcggac gtgttccggg atcggccgcc caacggtcag gcggtcgcca tcccgatcca
     1081 gtgtcgaata gaattcggcg acgccctcct cgagccattg cggcaggcgc gccgacgagt
     1141 ggttcaaaac aaggtgcaca tactcatgac aggcaatccg ggagttgccg cgcccggagt
     1201 ctctcatcac gatgtagttg cgttccggcc ccgcctggaa gtagcccgtc gtggtctccg
     1261 atagtcgaaa cggtcggaag tcggactccg atcgaaacaa caacacgcgg gttggcacag
     1321 gcggcgacgg ccggttgcca ggcaggctgc gaaacacgcc gtggatctcc tccagccgat
     1381 cgagcaaggt gcgtccggtg gcctcgccgg cgttcgtgag aagttcgaaa tgcgggcttt
     1441 ccaggcgcac ccatccagcg gcggccaccg ctgcaagcaa cagaaacaga acgatcaacc
     1501 ggtggaacac gattctctgg ccccagtata aggccaatag aaactttcga ttggaccgga
     1561 agcgcaggcg cccgctaggc tggagtcttg gcccatgtct gagccggact cgcccgcgtc
     1621 ccacgtggcg ctattgcacc gcgtaggcac catcgtgagc tccgagcgct cgctcgacga
     1681 aatgctcggg gaactcgtgg gactcaccgt gcaagtgacg gagtgcgacg cctgccccgt
     1741 ttatctgctt gcgggcatcc caagtccctc atgccgcgga actgggcacg ctgcgcctga
     1801 agttcggaga gggcgtcacg ggctgggtgg cggagcacaa ttcggtggta gctctgggat
     1861 ccaacgcctc cgccgacccg cgctttaagc gcctggcggg catggtggag gatccgtacg
     1921 aagcgttcct gtcggtgccg ctggtgagtg ggggcgaggt gaccggcgtc ctcaacgtcc
     1981 atcacaagga gccgcgcgcg cacgcagccg cggagatcac tctgcttgcg ttcatcggag
     2041 agcagatggg gggagccatc gccaagtcgc ggctggcgga ggaaaacgcc cgtctgctcg
     2101 aagagacgct cgagatgaaa cgccagctcg agatacggaa gctcgtggag cgggcaaagg
     2161 gcctactcca aacccgctac ggaatcagcg aggaggaagc ctatctgcgg atgagaaacg
     2221 agagccggcg gctgcgccgg ccgatgcagg acctcggtga ggcggtcatt ctggccgaag
     2281 agatggcgcg caaggccggg gaccaggacg ccaacggagg tcagcctccg ggctaggcga
     2341 gggcggttga caagtgtgtt cgggtccgag gatagatatc gttcttctct ggctgggccc
     2401 ttcgtggtga agctcgcaac agataccacc ggcaaagaac gggatgttga gacggcgaaa
     2461 acccgggact gactccattt cgccgccgtt gcgaaatggt gtctgtaccg ggttttccgt
     2521 gtccgggttg cgggtgcgaa tggattaact gccgagcatt gcccggcgtc gtagaggaaa
     2581 ggcatcgcgg cgagtgttgc cacgttgggt gatatgatgc gggacgccgg tcgcaacgac
     2641 gcgggcgagg cgagccatag ctatgcggag cgcgaaggcg ggcagtttcg catgccccga
     2701 aactcctgag gacgttcccc aagagagcac tcgtgcgagc aaaagcgatc attgttagta
     2761 ttctgttgct gggggccgga cgcggcggct atgcccagcc accttcagta ctcgcgcgag
     2821 tagtgagcct tgagataagc agggacgtga cccagagcga tgctcagctc cactggaaga
     2881 taacaaactc cctcgacgtt gcggtgtacg tttacgattt ctacctgtgg gggccagcgt
     2941 acagcctcgt gcgggttggg gatcgagtga ggatagatac aagtcccgtt atagagcaac
     3001 ggtcctgccc acccaacagg ttcccgcctg ttctgctgct agtcgtgggg ccacggcgaa
     3061 ccattacggg agatttttcc gaccctgaga taaaaggctt ggaaggtaaa caagtctcgc
     3121 tccgcatcgc ggtgggctcg gacccgtata gcgtggttgc agaggcgaag cgctttatgt
     3181 caagccgctg ccaacatagc ccttatgatg caatcgtgcg ttggggcaca attatcgaga
     3241 gcaactcact tcggttccag tagcgcgtgt gctcgggcag ccgggaacgg accattctct
     3301 ctcaccccga ccggactcag tcgtcacggt tcggtcagaa gcggaaggcc tgccgagcgt
     3361 tgcaggacgt cgtaggggaa ggcatcgcgg cgagtgttgc cacgttgagt gatatgatgc
     3421 gagacgccgg tcgcaacgac gcgggcgagg taagaaccat gcggagccgg aaggctggcg
     3481 attgcgttgc atgtccccga aactccaact gattcggcct gttggagtaa gatacttggc
     3541 atgcgtctcg tcataattct tctttgtgtg ctgctgctca cgatcgggtc tactcccgca
     3601 tggccgcagt cgcaagcaga actgaacgag caagcctgcg gtgcctacaa gaaggccgat
     3661 gcggaactga accgggtata caggcgggct ctggaagcaa gacatgacga cgcagcgttc
     3721 gtcgaggccc tgagggccgc gcagcgtgca tgggtggttt tccgggatgc tcacgtcagg
     3781 gcgatctatc cagatcgagc tccgcaagca tacggaactg cgaacccaat gtgcaagtgc
     3841 agtattcttg cctctcttac aaataggcgc gccaaggaaa tccgtgatat ttggattgat
     3901 ggcactgagg aaggagatgc gtgcggaggc agctcgccgg tcaggccgaa agactcccac
     3961 agcgtgaggg cgcctcgcag ataagacagg gggaaaagcc gggacaacac actcaatttc
     4021 cgtttccgtg tcttgcgggg ctgggtgtga attgattggt ttcatcgctt cttgccggcc
     4081 gacgacgtgc catctttgag cttcgccgtc ggattgcgat aggcgttcag gtagtcggcc
     4141 gtcgatccgg gacgaaaaca atttccctca acccccgcaa ctcccggtca caatcatccc
     4201 aaagtcatta actcattctt ttggagtgac ttacatccga atcacgccac tccatccccc
     4261 aggattacga tcccgccccg taacatgatg tcggtaggga aacggtggct cccgcttcct
     4321 tccccaaact ttccagaccc gaggttttcc caatgttttc tcttcaccag gtgtccgatt
     4381 ccgggattgg ctttgttcag tcaacactct cgggttaggc gagccagagg cggttacaat
     4441 gatggtttgc cgccgtttcg accgctcttc gccaatccac accttctcac catcgcggcc
     4501 aacttctggc ctcgacgcct cgatgaggtg cgatttccgg tggaggatac cttgttcgag
     4561 accgagccag gcgtgcgggt actcatccgc acgcagcgtc ccgaaggagc gtcgcgcgga
     4621 gaaatcatct tcgtgcatgg cctcgaagga tcgagtgagg gcggctacat gcgtagcgct
     4681 gcgcaggcag ccctggcggc gggctttgtc gctcatcgct acaatatccg gagctgcggc
     4741 ggcacc
//
LOCUS       DATIFQ010000227         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2372707, whole genome shotgun sequence.
ACCESSION   DATIFQ010000227 DATIFQ010000000
VERSION     DATIFQ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372707"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VLE22_10580"
     CDS             complement(<1..919)
                     /locus_tag="VLE22_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14894.1"
                     /translation="MKKLLAGLLVVLSGVGAAHLRAASQVVRLGGGSNELFNQIEQVL
                     AGLTEITGMKPVKKVAYDTIDKARVNQFLNERIKEFIKPEEIRAEELALKKFGFVPQD
                     FDLKKTSVELMTEQAAAFYDFRKKKLFILESAPEAVQQVALVHELAHALADQHFNLEQ
                     FIDLANKSDDSATARMAVMEGQATWLMSEYLARRTGQSLKTSPAIVQLMSRAAQMSQG
                     QFPVFDAAPLYMRETLLFPYSQGMLFQHALVEKFGQEAFARIFREPPVSSQQILHPEK
                     YFREVKPTEPELPSVSSEEDYEELTEGYIG"
     gene            994..1305
                     /locus_tag="VLE22_10585"
     CDS             994..1305
                     /locus_tag="VLE22_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HSB14895.1"
                     /translation="MESGVPEHEDEPVRTPITDVFDLHSVPDRDVRAIVEAYLEEVAT
                     LGFPCVRIIHGRGIGVRREIVRAVLSGCGFVETYGDAPAEAGGWGATVVTLRAAGQSR
                     R"
     gene            complement(1290..>2149)
                     /locus_tag="VLE22_10590"
     CDS             complement(1290..>2149)
                     /locus_tag="VLE22_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HSB14896.1"
                     /translation="IKKYSDDRAWRLVRVPWKPALRIEAEDAYLNFRSPGYVVARFIE
                     ATVPKGEKVLALSPVFEAYTTRDILIGYQAASNRTLIDVLWTAMIEDFRPTWRLKFHF
                     PARQLRKVRVIQTATAEADIWSVSEFRALHDGREFPRSPHWKIQAHPYPWDAWMAVDG
                     NPVTRWRSWQNLEPGMFLEVDFGQAELADSVWLDCSRDQYKIRLSLEGQLASGEWTRL
                     PAEPDNENVTTLPDLRPTAVAELKRRGVNYLLLWDHDYGAGEFRTNHAAWGMTPLGER
                     AGARLYRLD"
BASE COUNT          386 a          664 c          655 g          444 t
ORIGIN      
        1 cgccgatgta gccctccgta agttcctcgt aatcctcctc tgatgagacg gacgggagtt
       61 ccggctcggt aggtttcacc tcccgaaagt atttctcggg atgcaggatc tgctgggaac
      121 tcacgggcgg ctcacggaag atcctcgcga aggcttcctg cccgaatttc tccaccagcg
      181 cgtgctgaaa cagcatcccc tgggagtagg ggaacagcag cgtttcccgc atgtacaacg
      241 gcgcggcgtc aaataccgga aactgtccct gcgacatctg cgcggcccgg ctcatcaact
      301 gcacaatcgc cggcgacgtc ttcaacgact gtccggtccg gcgggcgagg tattccgaca
      361 tcagccacgt cgcctggccc tccattacgg ccatccgcgc ggtggcgctg tcgtcgctct
      421 tgttggcgag gtcgatgaac tgctcgaggt tgaagtgctg gtccgcgaga gcgtgggcca
      481 gttcgtgcac gagagcgacc tgctggaccg cttccggagc cgactcgaga atgaacaact
      541 tcttcttgcg aaagtcgtag aacgccgccg cttgctcggt catgagctct acgctggtct
      601 tcttgaggtc gaagtcctgc ggcacgaagc cgaacttctt gagcgccagc tcctcggcgc
      661 ggatctcttc aggcttgatg aactccttga tccgttcgtt gagaaactgg ttgacgcgcg
      721 ccttgtcgat cgtgtcgtag gcgacctttt tcaccggttt catcccggta atctcggtca
      781 ggccggcgag cacctgctcg atttggttga agagttcgtt ggaccctcct cctagccgca
      841 ccacctgaga cgcggctcgc aaatgggcgg ctccgacccc cgacagaaca acaagaagac
      901 cggcaaggag ttttttcatt cgtactttct gggaattgcg cttcctctca ggatacccga
      961 ttgcgcggtc tgccttgata cgatcagaat cgaatggaat ctggcgttcc agagcacgaa
     1021 gacgagcctg tccgcactcc gattacagac gtgtttgact tgcacagcgt tcccgaccgc
     1081 gacgtgcggg cgatcgtcga ggcgtacctg gaagaggtgg ccacgctcgg gttcccctgt
     1141 gtccgaatca tccacgggcg gggaatcggc gtacggcgtg agatcgtgcg ggcggtgctc
     1201 agcggttgcg ggttcgtcga gacgtacggc gacgcaccgg ccgaggccgg cggatggggc
     1261 gcaacggttg tgacgctgcg ggctgccggt cagtccaggc ggtaaagcct tgcgccggcg
     1321 cgctcaccca gcggggtcat cccccaggcg gcgtggtttg tccgaaactc cccagccccg
     1381 taatcgtggt cccacaacaa caggtagttg accccgcgcc gcttcagttc cgcaaccgcc
     1441 gtcggtctga gatccgggag agtcgtaaca ttttcgttat ccggttccgc ggggagcctc
     1501 gtccactcgc ccgaggccag ttgtccctca agactcagcc ggatcttgta ctggtcgcgt
     1561 gagcagtcaa gccacacgga atccgccaac tcggcttggc cgaagtcaac ctcgagaaac
     1621 attccgggct ccaggttctg ccagcttcgc cagcgtgtga ctgggttgcc gtcgacggcc
     1681 atccatgcgt cccaaggata tggatgcgcc tggatcttcc aatgcgggga gcgcgggaat
     1741 tcccggccgt catgcagagc gcgaaactcg ctcacgctcc agatgtcggc ctccgctgtc
     1801 gccgtctgta tcacacggac cttgcgcagt tgccgggctg gaaagtgaaa cttgagtcgc
     1861 caggtcggtc gaaagtcttc aatcatcgcc gtccagagca catcgatcaa agtgcggtta
     1921 gacgctgcct ggtagccgat caggatgtct cgtgtggtgt acgcctcgaa aaccgggctc
     1981 agcgcgagga ctttctcgcc tttgggtacc gtggcctcga tgaaccgggc gacgacgtag
     2041 ccgggcgacc ggaaattcag gtacgcgtcc tcggcttcga tgcgcaacgc cggcttccag
     2101 gggactctga cgaggcgcca tgcccgatcg tccgagtact tcttgatgt
//
LOCUS       DATIFQ010000228         2823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_840706, whole genome shotgun sequence.
ACCESSION   DATIFQ010000228 DATIFQ010000000
VERSION     DATIFQ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840706"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VLE22_10595"
     CDS             <1..297
                     /locus_tag="VLE22_10595"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HSB14897.1"
                     /translation="DIFRGRYRESLEVPKAIAANQPLVYRFPLPTTNHVFLPGHRMMI
                     QVQSSWFPLYDRNPQTFVPNIFWAKPADYKKATQRIYLSAGQASYVEIPLIRNR"
     gene            551..961
                     /locus_tag="VLE22_10600"
     CDS             551..961
                     /locus_tag="VLE22_10600"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSB14898.1"
                     /translation="MEIIVSFPGGTRVDTEIGGHVIKSDQPAYAGGQDSAPAPFSLFL
                     ASIGTCAGYYVLGFCSQRGISTDGIRIVQRLHKNPETKMIDVIDLDIEVPPEFPAKYY
                     DALVRSANQCAVKKHIENPPRFDVRTVVKAAAGA"
     gene            complement(1040..2059)
                     /locus_tag="VLE22_10605"
     CDS             complement(1040..2059)
                     /locus_tag="VLE22_10605"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HSB14899.1"
                     /translation="MKRLLPFLVLAACVSLTGLLVAGSAPPQGTPALRANPALPPVAI
                     PADNPHTDVKVQLGKMLYFEPRLSADNTISCATCHDPEMAWANHGATDVGIGGKRGNR
                     NSGTILDAAYMEFQFWDGRAKSLEEQAVGPIHNPVEMGETLENVVKKLNAIEGYRTKF
                     QAVFGTDATADGIGKAIAAFERTVLSGPSPYDRYMGGDKTAMSPAAIRGLAVFNTKGH
                     CAVCHNGPMFSNQWFHNLGLGMDRPDPDVGREAVTKNPADRGKFKTPGLRNVALTYPY
                     MHDGSMKTLKDVIEFYNKGGVANPNLDPAVKPLDLSEPEQADLIEFLKALTGTLPKIE
                     RPTLP"
     gene            2128..>2823
                     /locus_tag="VLE22_10610"
     CDS             2128..>2823
                     /locus_tag="VLE22_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HSB14900.1"
                     /translation="MKMTDTRPLFRPLCADIVSLFRSLSADQWERPTVAGRWRVRDLA
                     AHMLDTALRRLSYQRDQQRPASPSRPIGSDRDLVAWVNELNRTWVAAAERLSPRVLTD
                     LYANISAQLCAFVEGLPDDAPALFPVSWAGEEDSAGWFDIGREFTEIWHHGAQAREAV
                     GAGLYPEPAWLRAVIEISLHALPHAYRDVAAQNGTSVLIEIAGPAGGTWTLRRVGARW
                     AVGEAPASRVDATV"
BASE COUNT          511 a          892 c          869 g          551 t
ORIGIN      
        1 gacatcttcc ggggccgcta ccgcgagagt cttgaagtgc cgaaagccat cgccgcgaac
       61 caaccgctgg tctatcggtt cccgctgccc acgaccaacc acgtgtttct gcccgggcac
      121 cgcatgatga tccaggtgca atcgagctgg tttccactgt acgaccggaa cccgcagacg
      181 tttgtgccga acatcttctg ggccaaaccg gcggactaca agaaggcgac ccagcggatc
      241 tacctctcgg ccgggcaggc gagctacgtg gagatccccc taatccggaa tcgttagagc
      301 ttgtttcacc gcggagcacg cagagcacgc agggcaaatc ccagtttttc tccgcgtgcc
      361 cctgcgtgct ctgcggtgag acacgcagtg acgcctgccg caagcacggc gtaactgcca
      421 gcaaaagcct ctcaacgccg aaggcgcgga aaccgcagaa ggtgtaagtc agttcctccc
      481 gttccgcgtg ttccgcggtg aaaggctttt atgcgtgtca ccgtttgggc gtattctgaa
      541 tctcaccaac atggaaatca ttgtttcttt cccgggcggc acgagggtag acactgaaat
      601 tggcggccac gtcatcaaga gcgatcagcc ggcctatgcc ggaggacaag actccgcgcc
      661 cgctccattt tcattgttcc tggcttcgat cggtacctgc gcgggatact acgtcctggg
      721 cttctgcagc cagcgtggta tttcgaccga cggaatccga atcgtccagc gactgcacaa
      781 gaaccccgag acaaaaatga tcgacgtgat cgatctcgat atcgaggtgc cgccggagtt
      841 cccggcgaaa tactacgatg cgctggtgcg gtccgccaac cagtgcgcgg ttaagaagca
      901 catcgagaac ccgccgcggt tcgatgtgag gaccgtcgtc aaagcggcgg ctggagcgta
      961 gccagataca ggatcacgaa tgaaagatga agaatgaaag gatcttcatc tctcattctt
     1021 catcttgttc ggttgaccct taaggcagcg tcggccgctc gattttcggc agcgtgccgg
     1081 tcagcgcctt gaggaactcg atcaggtccg cctgctccgg ctcggacagg tcgagcggct
     1141 tcaccgccgg atcgagattc gggttcgcca cgccaccctt gttgtaaaac tcgatcacat
     1201 ccttcagcgt cttcatcgat ccgtcgtgca tgtacggata tgtgagcgcc acgttccgca
     1261 gtccgggtgt tttgaacttg ccgcgatcgg ccgggttctt cgtcacggcc tcccggccga
     1321 catccgggtc gggccggtcc atcccgagcc cgaggttgtg gaaccactgg ttcgagaaca
     1381 tcggaccgtt atggcacacc gcgcagtgtc ccttcgtatt gaacacggcc aggccccgaa
     1441 tggccgcggg cgacatcgct gtcttgtctc cgcccatgta acgatcgtag ggcgacggcc
     1501 ctgagagcac cgtgcgctcg aacgccgcga tcgctttgcc gatgccgtcg gccgtcgcat
     1561 ccgttccgaa caccgcctgg aacttcgttc gatacccttc aattgcattc aacttcttga
     1621 cgacgttctc gagcgtttcc cccatttcca cgggattgtg gatgggaccg acggcctgct
     1681 cctcgagtga cttggcgcga ccatcccaga actggaattc catgtacgcc gcgtccagga
     1741 tggttccgga gttacgattg cccctcttgc cgccgatccc cacgtcggtc gcgccgtgat
     1801 tggcccacgc catctcaggg tcgtggcacg tcgcgcagct gatggtgttg tcggcagaga
     1861 gtcgaggttc gaagtacagc atttttccga gctgcacctt gacatcggtg tgcgggttgt
     1921 ccgctggaat ggcgaccggg ggtaacgcgg gattggctcg cagtgccggc gtcccctgcg
     1981 gtggagcgct tccggcgacc agtaaacctg tgagggacac gcaggcggcg agaaccagga
     2041 agggaagaag ccgcttcata aatcctcccg ggaagattct gtaacgctga gctagtctac
     2101 tcccgggagg cgtactggag gactcgaatg aagatgaccg acacccgccc gctcttccgt
     2161 ccgctctgcg ctgacatcgt gtcgctgttc cgctcgcttt ccgcggatca atgggagcga
     2221 ccgacggttg ccggccggtg gcgggtgcgc gatcttgccg cacacatgct ggacacggcc
     2281 ctccggcggc tctcgtatca acgcgatcag cagcgccccg cctcgccatc acgtccgatc
     2341 ggcagcgacc gcgacctggt cgcgtgggtg aacgaactca accgaacgtg ggttgccgcg
     2401 gccgagcggc tcagccccag ggtcctcacc gacctgtacg ccaacatcag cgctcaactc
     2461 tgcgcgtttg tcgagggcct gcccgacgat gcgcccgccc tgttcccggt gtcgtgggca
     2521 ggcgaggagg actcagccgg gtggttcgat atcggccggg agttcacgga gatctggcac
     2581 catggtgccc aggcccgcga agcggtcggg gcgggtcttt atccagaacc ggcgtggttg
     2641 cgcgcagtga tcgagatatc gctgcacgcg ctgccgcacg cgtaccgcga cgtcgctgcg
     2701 cagaacggca cgtccgtcct gatcgagatt gcgggtcccg cggggggcac ctggacactc
     2761 cggcgcgtgg gcgcgcggtg ggcggtcggg gaagcgcccg cgtcgcgagt ggacgccacg
     2821 gtg
//
LOCUS       DATIFQ010000229         3980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_650262, whole genome shotgun sequence.
ACCESSION   DATIFQ010000229 DATIFQ010000000
VERSION     DATIFQ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650262"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2256
                     /gene="bamA"
                     /locus_tag="VLE22_10615"
     CDS             <1..2256
                     /gene="bamA"
                     /locus_tag="VLE22_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HSB14901.1"
                     /translation="SLEVTFNVKEGPKVKVGEIEITGNNVMGDRAVRRAMKNLRPIGI
                     PKSILFESLFSKAYDLSKLEQDKEMIRNYYQERGYFTAKALEHEVKIRDVGGGWFRIP
                     LFYPNKPGKRADLRIPVEEGPQYKLNKINFVGMKLFRTPDLLAQQLFQMKVGDVFSTG
                     KLRKGLEEMRKLYGQFGYIDFVPEPDFNILPNSDKITLTLNIDEGKQFFIRRIDFSGN
                     TTTRDKVIRRELLVDEGDMFSTRLWELSILRLNQLGYFEVLKENEAADIKRDTRTNTV
                     DITLKVKERGKNSVGLQGGVSGIAGSFVGFNYATNNFLGLGETLSLDAQLGDRLRAVT
                     FGFTEPYFLDRPMQVGFTVYTQRFNFDQAREVSLLSGRNLIPLFEAVGTGNLLNYISN
                     GHGFTVYSSYPLKRSFARVGLTYGYDKSNITTLNDTSSSYFRYYNFQGLGGPNSLTGI
                     VTSKLIPSYSYNTVNHPITPTGGRSLYIATEFAGSFMGGNVNLIRPTITATYFRPALF
                     KGHVLGMRVLGSFLTGYGGKLAPPFSRYYMGGEQDIRGFDIWGVSPMGFVPSEAGITV
                     LNNDGSARTQKVIQDGKEVYTTVTQSIPIYQLIFPGGDTQAVSNVEYRIPIVGPVTLA
                     AFFDTGLNKISRPAQLALNPGRVTELNSKFPQAGFNERAVIAPGTQKIRTSAGIEFQV
                     LMPVVNAPFRLYWAYNPTLVQQFLQPPIVLDRSAFPNEETFLYAVQKYGSPIPFFEKR
                     RTWRFTIGRTF"
     gene            2300..2911
                     /locus_tag="VLE22_10620"
     CDS             2300..2911
                     /locus_tag="VLE22_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSB14902.1"
                     /translation="MHSRVYVLPCLAFAAVLAHGQQAQTKVGIIHVQNAIISTKDGQK
                     IAGELQARFDPRRKEIEKRQTEIQALRDQLNRGSNTMSDEAKQTLIREIDQKTRSANR
                     ASEDAQADFEQDRDRVLQELGQRMMVVIDKYARDNGYALIVDVSSPQTPVLYASNTID
                     ITNDIVALYDKNAPSAAPSAPAAAPPPAAPAKPAGLPPATKKK"
     gene            complement(3086..3661)
                     /locus_tag="VLE22_10625"
     CDS             complement(3086..3661)
                     /locus_tag="VLE22_10625"
                     /inference="COORDINATES: protein motif:HMM:NF039962.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6030 family protein"
                     /protein_id="HSB14903.1"
                     /translation="MQIPGMLSLALLFGTASFGQVLEIFDDPDKACALLESEGFPGRE
                     WTLRPYGLQCFTTRTKTEEVSGRAQAAPVDDSWPALEVVPQGSIAYAASGESLTRVSR
                     LKLIVFLVPDQPNKVLMAEYERLATVLLTRIGLNVSDDLRKAIRNMLPWRRALRGANI
                     TFDPGPKPSSLLVLTVRDPRIRVETIPRVRD"
BASE COUNT          847 a         1236 c         1190 g          707 t
ORIGIN      
        1 tcgctcgaag tcaccttcaa cgtcaaggaa ggccccaagg tcaaggtggg cgagatcgag
       61 attacgggca acaacgtgat gggcgaccgc gccgtgcggc gcgccatgaa aaacctgcgc
      121 cccatcggca tcccgaaatc gatcctgttt gagagtctct tttcgaaggc ctatgacctg
      181 agcaagctcg agcaggacaa ggagatgatc cggaactact atcaggagcg cgggtacttt
      241 accgcgaagg ctcttgagca tgaggtaaag atccgcgacg tcggcggcgg gtggttccgg
      301 attccgctgt tctacccgaa caaaccgggt aagcgcgccg acctgaggat tccggtcgaa
      361 gaaggcccgc agtacaaact gaacaagatc aatttcgtcg gcatgaaact gttccgcacg
      421 ccggacctgc tggcgcagca actgttccag atgaaagtgg gcgacgtctt ctccaccgga
      481 aagctgcgga agggcctgga ggagatgcgc aagctttacg gccagttcgg ctacatcgat
      541 ttcgtgcccg agcccgactt caacatcctc cccaactcgg acaaaatcac cctcacgctg
      601 aacatcgacg agggcaagca gttctttatc cgccgcatcg acttttccgg gaacacaacc
      661 acccgcgaca aggtgatccg ccgggagttg ctggttgacg agggcgacat gttcagcacg
      721 agattgtggg agctgagcat tctgcggctc aaccagctcg ggtacttcga ggtgctgaag
      781 gaaaacgagg ccgccgacat caagcgcgac accaggacga acacggtgga catcacgctg
      841 aaggtgaagg agcgcggcaa gaactcggtc ggtcttcagg gcggcgtgtc gggcatcgcc
      901 ggaagcttcg tcggattcaa ctatgcgacc aacaacttcc tcggcctggg cgaaacactg
      961 agcctggacg cgcagctcgg cgaccggttg cgagcggtga ctttcggctt caccgagcca
     1021 tacttcctgg accggccgat gcaggtgggc ttcacggttt acacgcagcg attcaacttc
     1081 gaccaggcgc gcgaggtctc gctgctgtcc ggacgaaacc tgattccgct attcgaagcg
     1141 gtgggaaccg gcaaccttct gaactacatt tcgaacggcc acggcttcac ggtttattcg
     1201 agctacccgc tgaagcgctc gttcgcgcgt gtcgggctca cctacggtta cgacaagtcg
     1261 aacatcacca cgctgaacga tacctcctcg tcgtacttcc ggtactacaa cttccagggc
     1321 ctcggcggcc cgaactcgct caccggaatc gtcacgagca aattgatccc gtcgtactcc
     1381 tacaacacgg tgaaccaccc gatcacgccc acgggcggcc ggagcctgta catcgccacg
     1441 gaattcgcgg gcagctttat gggcggcaat gtgaacctga tccgtccgac gatcaccgcc
     1501 acctatttcc gtccggccct gttcaagggg cacgtcctcg gcatgcgcgt gctcggcagc
     1561 ttcctgacgg ggtacggcgg aaagctcgcg cccccattta gccggtacta catgggcggc
     1621 gagcaggaca tccgcggctt cgacatctgg ggcgtcagcc cgatgggttt cgtgccgagc
     1681 gaggcgggca tcacggtgtt gaacaacgac gggtccgcgc gtacgcaaaa ggtcatccag
     1741 gacggcaagg aagtgtacac gaccgtcacg cagtcgattc cgatctacca gttgattttt
     1801 ccgggcggcg acacgcaggc ggtcagcaac gtagagtacc ggattccgat cgtcggtccg
     1861 gtaacgctgg cggcgttctt cgacacgggg ctgaacaaga tttcgcgtcc ggcgcagttg
     1921 gcgctcaatc cgggccgggt gaccgaactg aactcgaagt ttccgcaggc gggcttcaac
     1981 gagcgggcgg tgatcgcgcc cggaacccag aagattcgga cgtcagcggg catcgagttc
     2041 caggtactga tgccggtcgt caacgcgcca ttccggctgt actgggccta caatcccacg
     2101 ctggtgcagc agttcctgca accgccgatc gttctcgacc ggtccgcctt cccgaacgag
     2161 gagacgttcc tgtacgcagt acagaagtac ggcagcccaa tcccattctt cgagaagaga
     2221 cggacatggc ggttcaccat cggccggacg ttctgacgtg atatggtact atcgagtgtt
     2281 tctttcagga gtgtccacag tgcatagcag agtgtatgta ttgccttgtc tggccttcgc
     2341 ggccgtcctt gcgcacgggc agcaggctca gacgaaggtg ggaattatcc acgtccagaa
     2401 cgcgatcatc agcacgaagg acgggcagaa aatagccggc gaacttcagg cccgcttcga
     2461 tccccgccgt aaagagatcg agaaaaggca gacagagatc caggcgttgc gggaccagtt
     2521 gaaccggggc agcaacacga tgagcgacga agctaagcag acgctcatcc gcgagatcga
     2581 ccagaagacc cgctcggcca accgcgcttc cgaggatgcg caggcggact tcgagcagga
     2641 ccgcgaccgc gtgcttcagg aactggggca gcggatgatg gtggtgatcg acaagtacgc
     2701 gcgcgacaac ggctacgcgt tgatcgtgga cgtgagttcg ccgcagaccc cggtgctgta
     2761 cgcctccaac acgatcgaca tcaccaacga catcgtcgcg ctgtacgaca agaacgcgcc
     2821 gagcgccgcc ccgtccgcgc ccgccgcggc gccgccgccg gcagccccgg cgaaaccggc
     2881 tggactgcca cccgcgacga agaagaaata gtgttcgcct atacaggcgc cgaagaaccc
     2941 gtgggcattg agcaccatca gggtgaagtg ccacagtccg attcaccaca gagaacatcc
     3001 gcgttagcct ccattccgtt tttggccgta gacttcagaa acctcggcgg aggctctgct
     3061 atcgacctga gggcagccga gaccgtcaat ctcgcactct gggaatcgtt tcgacgcgga
     3121 ttcgcggatc gcgcacggtc aacacgagaa ggctagatgg tttgggcccc gggtcgaagg
     3181 tgatgtttgc gccgcgcagc gcccggcgcc acggcagcat attccggatt gccttccgca
     3241 ggtcgtccga aacgttgagc ccgatccgcg tcaagaggac agtcgccagc cgctcatact
     3301 ccgccatgag aactttgttc ggctgatccg gcaccagaaa gacgatcagt ttcaggcggc
     3361 tgacacgagt caggctctca ccagaggcgg cgtaggcgat gcttccctgc ggaaccacct
     3421 cgagcgccgg ccaggaatcg tccaccggcg ccgcctgcgc gcggccgctt acttcctccg
     3481 tttttgtgcg tgtcgtgaaa cattgcaggc cgtaagggcg caaagtccac tcacggcccg
     3541 ggaacccttc agactcgagc agcgcacatg ccttgtcggg atcgtcgaag atctccagaa
     3601 cctggccaaa ggatgcggtg ccgaacagga gtgcaaggct caacatgccc ggaatctgca
     3661 tgccaccacc tgatgcaacc gagcatagca tcggcggggt gccccgctag ctttcgatct
     3721 cgctccgcct ggatctcccg ctgatgcccc accagcatat ccttcggcga gacatagccg
     3781 accgcgctat tcatgcgaag ggtttatagt gctcgacgaa acccggtcca gacgccgcgc
     3841 atctcccagc aagagcggcg ttggcggggc ggcgcgactt aacattccgc cacaacatct
     3901 gctggcgtcc accccgtcat gcgaacggtg aagtgccatg accgaggatc tgtctcgtac
     3961 gacagcgccc attctgggtc
//
LOCUS       DATIFQ010000230        32007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1224621, whole genome shotgun sequence.
ACCESSION   DATIFQ010000230 DATIFQ010000000
VERSION     DATIFQ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 32007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32007
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224621"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VLE22_10630"
     CDS             complement(<1..513)
                     /locus_tag="VLE22_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14904.1"
                     /translation="MKMKAPRSIGVSGRDLLLRKATALFNEQCAIAILANLPQRINSE
                     DRRAHVAALATQPTKQLREFAEMLRRNVEKMRDDLGIKLDQGKLLALMDDLQNSAPSD
                     YACYIPVWRLSEWLSRYDSLGLDPHWRVSIDPHCIGKKYPGGWEIRALEATMFEDMAA
                     LFNMARHAHLE"
     gene            966..1685
                     /locus_tag="VLE22_10635"
     CDS             966..1685
                     /locus_tag="VLE22_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSB14905.1"
                     /translation="MSKVATVAVCLSLLTFPAFADGEANCAGLPTARDLNKLLVAAAG
                     DDIGGLFNGERMWAAVVNRSGEICAYATSTSDPTQVWPGSQAIAKSKAYTANAFSLDT
                     LALSTARLYTFTQPGHSLWSLGQSNPFEPKWLAPPRGNSGGRNQLAGGLIFFGGGLPL
                     YQGGKIIGGLGVSGDTSCADHEVAKKVRDAAGLNPPGGKLADDITYSSADGASAFTHP
                     LCPNTKRNGTFLGNEAPASGY"
     gene            1782..2924
                     /locus_tag="VLE22_10640"
     CDS             1782..2924
                     /locus_tag="VLE22_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HSB14906.1"
                     /translation="MNFRTLAIHAGQGPDPAYGAVMPPIYQTSTFAFRGVNEPGPFDY
                     SRSGNPTRKALETCLARLEGGVAGFAFGTGMAAETTVLMLFDAGDRIIVHNDLYGGTY
                     RLLTTVLANKGIGVDFVDLRRSDLVRQAIRPETRAIWIETPTNPLMNLVDMAEVAGIA
                     RENGLLTICDNTFLSPYFQRPFEFGIDIVVHSTTKYINGHSDVVGGAVVVNNDALAER
                     IGYLQNALGTCAGPQDCFLVLRGIKTLAVRMEEHNRNALEIAHWLEQHKKIAAVLHPG
                     LPSHPQHDLALRQMRGFGGTFSFRVTGGRTEVFRLLGGVRLFTLAESLGGVESLIEHP
                     ATMTHASVPQEERERMGITDNVVRVSVGIEDVQDLVADLDQSLSRV"
     gene            2957..3712
                     /locus_tag="VLE22_10645"
     CDS             2957..3712
                     /locus_tag="VLE22_10645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HSB14907.1"
                     /translation="MLRGLIHGILGKFGYDVISTRGRYASDGLFTLHSDRFREDPAFR
                     RAYARGVRAGWGYDPRFTWRVHVALWAAKAALRVPGDFVECGVNAGFISSAIMEYLDW
                     RNVDKKFYLVDTFTGPVFLQYSAAEVAAGRVEAARNALAAGAYVTDLERVQANFAEWP
                     SAIVVQGTVPDVLPRLPVSSVALLHIDLNCAGPERAALEYFWDRLSPGALVLFDDYTY
                     FGQQCQGDAIDEAARQLGATVLSLPTGQGLIIK"
     gene            complement(3745..7008)
                     /locus_tag="VLE22_10650"
     CDS             complement(3745..7008)
                     /locus_tag="VLE22_10650"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB14908.1"
                     /translation="MQFLHRFRKLVLFLAGSALLYAQTGTSSITGTVTDATSSAIPNA
                     GIVLVNEESGARFVTRTNEAGNYRLASLLPARYRIEATAAGFEKASRGGLVLAVSQVL
                     PVDLTLQVGQASETVTVTETVPVAETQSSSVGQLVNRKMVSGLPMPNRAATSLVALAP
                     GVVMIDTGQGAENYPVFSVAGGRARNQNFTLDGGNVTNAVGLTRPQQMTSLPMDAMQE
                     FRVIGNAYSAEYGHSTGGIITLSTRSGTNQLHGSVFEFLRNSALDARNFFAAERAPIR
                     MHQFGGSLGGPIRKDKTHFFATWEQTRQLTSTIDLQTVPDLAQRAGDFFGLRDGAGRV
                     IPIYDPSTTVGRERQPFPENRIPLDRFDPVALAALSYWPSPNRTPGPAGGSNYAANAD
                     SDLHRNIFVAKVDHQLRSNDQLTVRYYLNDSFIGNRGSFPRREAAPDANVNDVRIQSV
                     LAGHTHTFSPNLISELKVSFFQRRFIDERYGFGENLAGAIGLTGVSAAAFPTFTIPGY
                     ASLGGQVGRHQTPIRDTQILEAVSWFRGRHALKFGVEHRRGRNTEVRDRSSAGLFGIT
                     PLITGKPGTSGAGDAFASFLLGEVNSASVNVSDQITSRAYYLAGYVQDDWRATDHLTL
                     NFGLRWESELPRRVDEDRQNSFDLSAINPVSGTPGIVTFSGRDGVPRQAFNTDWNNFG
                     PRVGFAYRLPFQSETVIRAAAGVFYGSTVSNTIGDTASTGFSTAASLVVPQADLLSAM
                     QLRNGFPAFERPPLDARFGAVPLGRRPYTAVGFFERNRPTPVSYQYNANIQRVVASET
                     VVEAGYIANVSHHLTANDLSLNQVAPELMGPGDAQARRPFPQFSNVYIINPAIGNSTY
                     HAGYIRAEKRFARGLSFLAHYTFSKFLDDVVSSNEYGDPQSYMDAYNRRLDKSLSGTD
                     VPHRTVLSILYEVPRIRSNRYLNIVGGGWKLGGFGTWQSGAPFTVTTLANTTNAFSAG
                     PLRPDLIGAAELPGAQRSLERWFNTDAFRNPAQFRFGNAPRSVLRGPFQQSVDLTVAK
                     EFEVTERYRVDLRGEFYNAFNHANFDLPGHTLGAADFGTILSARPPRTVQLGLRLSF"
     gene            7412..8629
                     /locus_tag="VLE22_10655"
     CDS             7412..8629
                     /locus_tag="VLE22_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB14909.1"
                     /translation="MERRTLLKAAALLGAPAALPGEDLYRRDPEKYWRRIREEQFLLP
                     GWRSFLNNGSLGIAPRPVLKAVNDYLERSAALMVEEYPRWGYETLDEHRQELAEFFGC
                     KTEELALTHNATEAMNIVANGLDLKEGDEVLSTDQEHPGGLCCWLQRQARHGMTVRQV
                     AIPLPPKNQGQLADLLIGSIGPRTRVLSFSGITTMTGLIFPIRDICLAARAKGVITVV
                     DGAHMQGQIPFRLDELGCDFLAASPHKWLYTPAGCGFLYGREEMLDRLWVNVATAGWD
                     KKELKAARFMMVGTNNRAIFEGYIAGLRFLKQLGPERVYTRIHQLGRMCFEKARGTPG
                     VEMLTPDDDLMFAGLVTMKLKDGHWKRVQEFCAKKRIWVLQSSRSRISTHIHTRPQDL
                     DEFFEILRGVTAA"
     gene            complement(8632..10719)
                     /locus_tag="VLE22_10660"
     CDS             complement(8632..10719)
                     /locus_tag="VLE22_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSB14910.1"
                     /translation="MRHSAALLALLLSPAAFAQEPFTLEQALSSPFPSELTAAPSGGA
                     VAWVLNARGVRNIWVAEPPAYKSRPVTSYSSDDGQEISGVAWTPDARSLIFVRGGSAN
                     AQGENPNPLSDPAGAEQALWIVPLSGGEPRRIDDGSAPAVSPKGDRIAYLKKGQIWSA
                     PLAGDEKPAQLIRARGQCGSLRWSPGGVLLAFVSARADHSFIGVYDPAAKTLRYLDPG
                     LDLDSEPAWSPDGRSIAFLRVPNSRVLAIFGPKRSAEPWSVRVADVANGRGREIWRAK
                     PGAGSVFHGVIAENQLLWAAGGRIVFPWEAGGWTHLYSVPVSGGDTLPLTSGAFEVEH
                     VVVSPDRSEVIYSSNQDDIDRRHLWRVPVAGGRPRAITSGSGIEWSPVLLGGGATVAF
                     FRSDARRPARAAVLNEDGPRDLAPETIPSGFPGALVIPEPIEFPAADGMKIRGQLFLP
                     SGVRQGERRPAVIFFHGGSRRQMLLGGHYMDYYHRTYALNQYLASRGYVVLSVNYRSG
                     TGYGLEFREALNYGAAGASEFNDVLGAGLYLRNRPDVDPARIGLWGGSYGGYLTALGL
                     ARASDLFAAGVDLHGVHDWRTETRVYVSSDDLDTQQRALRVAFESSPLAHMSGWRSPV
                     LLIHGDDDRNVEFRQTVALAEALRKQEVDFEQLVFPDEIHGFLTHARWLEALQAAAGF
                     FDRHLTSGAGSSR"
     gene            10803..11993
                     /locus_tag="VLE22_10665"
     CDS             10803..11993
                     /locus_tag="VLE22_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB14911.1"
                     /translation="MDERQEAIVRKALDSFQIEIPSWGFANTGTRFGKFVQAEAATTI
                     EEKLSDAGQVHQFTSCCPSVAVHVLWDFPEGRKSVPEAMALAAKYGVRIGAINPNVFQ
                     DQIYKHGSLGSPDPEARAMALAHILDCVEIAKETGSRDLSLWFIDGSSYPGTANIRHR
                     KQWFADGLRAAHERLAANQRMLVEYKPFEPAFYHTDIADWGMALLLARAAGPQARVLV
                     DTGHHYQAQNIEQIVAWLLSEGMLGGFHFNDRRYADDDLTIGSIDPYQVFRIFHEIQF
                     HEWETGKRGDIAYMIDQSHNLKQKIEEMIQTASTAQELWAKASLVDHEALARSQAKCE
                     LIDAEEYLKAAFATDVRPALRAWRKSKGLAEEPLAAFRASGYLERAARERGERNRGAV
                     ASYA"
     gene            12019..12399
                     /locus_tag="VLE22_10670"
     CDS             12019..12399
                     /locus_tag="VLE22_10670"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB14912.1"
                     /translation="MDALVRHEGEAPRERSTCGWRHLLISRQDRGRAAAWAHAVDIDG
                     AREHYHKQGTELYYVLEGEGSVVLDGVEHPVRKGSLVQIPPGVVHGARGRMRVLVIGI
                     PDISDEDLYFPEDAAEHGPTVQGD"
     gene            12399..13490
                     /locus_tag="VLE22_10675"
     CDS             12399..13490
                     /locus_tag="VLE22_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14913.1"
                     /translation="MAGRRRKSGRACAIVVLLCCAPAAWPEEEIAEGVAPRVRPLSAS
                     DTATATPTSEPWIDSNLWLVRSLTGHSRAPVWLHFRVDGGSGDDYARAIADTAAAGGR
                     WAVELDDGFQADLRSGQSEALAKWGRIVNYLRFFEDHRGWREFVPSGPLGIVYDPAGK
                     YVDISNEYLNLIARRRIPYRVIHRADLGAGALGGLKAVLAIDASPPRTGERALMESFA
                     EAGGLVVTGPGWGKAPAGGEGYEVENSGKGRVAIYREDPPDPETVSRDMLTLIGRANT
                     GIRLFNGPSVLCHVTTGEAGARLLVQLINYATLPAEAVSLRVDGEYKAARVLSPDAPP
                     AELAVELAGGRTQVSLPAFSVYAAVVFEK"
     gene            13506..14699
                     /locus_tag="VLE22_10680"
     CDS             13506..14699
                     /locus_tag="VLE22_10680"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB14914.1"
                     /translation="MKDGCNGLTRRQWLAGAGVALGAASLGRGQGPASVPPVSIGKYA
                     SYDDDLVGRLAKQFDQAGGLGGLVRGKTVTVKLNLTGGGRFPGCTPGQTHWVHPKLVG
                     ACCYLFGKAGAKRVRLVEGTYEGESLQDKMLDGGWDVKAIQSAAPLVEFEETNTLGSG
                     KRYSRLRVAKPYIFPAFDLNHTYEDTDVFVNLSKLKQHEECGITLSIKNMFGVAPNSI
                     YGDDAGVDEPNENPRKGREHVLHLGRRQPSKSAPGEVNPASSRYEGHRVPRICVDLSA
                     ARPTDLAIVDGIETCVGGEGPWVKGSKHVQPGVVILGKNAVSTDTVATAVMGFDPRAK
                     RGEGPFQVDYKSHPQMPGDPKQADNPMLLAEAAGLGSADLSRIEVAGVSIKDALFDFE
                     KHRKG"
     gene            complement(14732..15922)
                     /locus_tag="VLE22_10685"
     CDS             complement(14732..15922)
                     /locus_tag="VLE22_10685"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14915.1"
                     /translation="MSSDVSRRSFIAGAVTAASASRVLGANDRIRLGIIGTGGRGSHL
                     MNMANLAGGIEWVAVCDAWDVRRDEAAKRIGKPVEKYADYRRMLDRKDIDAVIVATLD
                     HLHAGMTVDACRSGKDVFVEKPMTSQPLQGLDVVRAVRETNRIVQVGVQQRSLAHFIE
                     AKQKFFDSGLVGQVNMVRTIWNANGGYLTPVPPGMEQKPAGLDWDAVLGWLPKIPWDP
                     KRYFNRFAYWDFATGGQTGGLFVHMVDVVHWFLNIRKPVSAVALGGIYQFDDGRDTPD
                     NVNFILDYPEKLTVTFEATITDMIPRDSADIVFMGTGGRFHIFRYGYRYIPATKNSEV
                     GEIAAGGTAEVPHMKNWLDCIRSRKQPNADVVEGHYSAMACHIGNIAYKEKRRVEWNK
                     DWDA"
     gene            complement(15919..16794)
                     /locus_tag="VLE22_10690"
     CDS             complement(15919..16794)
                     /locus_tag="VLE22_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB14916.1"
                     /translation="MFQIGFHTDAFNSSYWSFEQCLKWAQDNGLHFIECGAIDGVSWI
                     HGLGYQPHVALWEDPVLLRRKMDRYDVRMSQIDAAFPLSLPEGASLGVEYVLHTIRWA
                     KLAGCPRVDTTDDRCKPAGMTEREALDHMRRIYRQFVPVAEAYQITVNVEPHGYFTTK
                     PDFMAEMLAFCDSPFLRMNMDTGNTFIAGQDPVAFLVRLKDQVSHVHVKDVSESLAAA
                     ARGDLTGIAVSHCAIGEGVNADNIRRCLTILAESGYDGVLSMECEGQGGPMIERSLVW
                     LRGLVEKLNQGVSIL"
     gene            complement(16966..19116)
                     /locus_tag="VLE22_10695"
     CDS             complement(16966..19116)
                     /locus_tag="VLE22_10695"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HSB14917.1"
                     /translation="MDFTRRQFIGASAALAAGSAQVQDQPAWCDKPMRWAQLTLVEDD
                     PGKYDPKFWLDYFRRIHADAACLSAGGCVAYYPTKVPFHHRSRWMGETDPFAELYEGC
                     RQLGMVVVARTDPHATYQDVYEAHPDWIAVDAEGRKRRHWASPEMWVTCTLGPYNFEF
                     MTEVTKEIVSLYKVDGIFSNRWAGSGMCYCEHCQRNFKDYCGMELPRTRDPLDPARRN
                     YIIWQQKRLFDLWRLWDGEIRKINPNARFVANAGGGALSDLDMKTIGELAPTLFADRQ
                     ARIGLNAPWAAGKNGKEYRATLGRKAIGGIFSVGVVQPYRWSDSVQNGAEIRIWVADG
                     LANGLRPWFTKFSGTIHDSRWLKTVEDIYNWQYRHERYLRNEEPLARVGMVYSQQTAW
                     FYGGERATQKVEDHTRGLYHALIEARVPFEMVHDRLLEPDRLARYKLLLLPNIAALSD
                     TQCDQLREYIRRGGSLLATFETSLYNEWGTRRPDFGLSDLFGVWYRGRVEGPVQNSHL
                     RLEHDTRHPILAGLEDAPRIIHGVYQVEVGARESFPNSPLTLIPSYPDLPMEKVFPRV
                     PKTSIPEVYLREIGPSRIVYFPWDIDRVFWEVMCVDHGRLLGNAVAWAMNEEQPVTVT
                     GPGVLDVTVWRQKDSMTVHLVNLTNPMMMKGPFREIIPLTEQKVRVRLPQGVRAKKVQ
                     LLESGRTPRFEESDGTLSLVVPSIRVHEIVAIDL"
     gene            19301..20554
                     /locus_tag="VLE22_10700"
     CDS             19301..20554
                     /locus_tag="VLE22_10700"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HSB14918.1"
                     /translation="MDVPREGVARKKLIRRIAAGTVVAAAIPLITIGLARLKPAAPTV
                     EKATVWMDTVKRGPMTREVRGLGTLVPEEMLWIPAPADGRVERILVRPGAAVDAGTVL
                     LVLSNPELDLAALEAEYQVKAAEAKYKDLKVQLESQRLTQQAEVARVQSEYHQAKLKA
                     DRDEALAKEKLLPDLDLKISVATAAELANRHSIEEKRLDIQSDSVDAQLAVQRAEVDK
                     VRALAGLKRTQVEALRVRAGAVGVLQELPVEVGQKLVAGTILAKVAQPERLKAELKIP
                     ETQAKDVQLGQKVAVDTRNGVIPGRVSRIDPAVREGTVTVDVKLEGSLPQGARPDLSV
                     DGTIELEHLADIVYVGRPAFGQPNSAVTLFRTDADGKGASRVHVKLGRASVNTIEVVE
                     GLRPGDQVILSDMSAWDAQDRVRLN"
     gene            20584..21288
                     /locus_tag="VLE22_10705"
     CDS             20584..21288
                     /locus_tag="VLE22_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB14919.1"
                     /translation="MNSTDTQLIKLEGVTKVFLTDEVETHALAGVHLEIRKGEYVSIA
                     GPSGCGKSTLLSILGLLDTPTDGRYWLNDRPVESLKPAERARIRNREIGFIFQAFNLI
                     GDLTVYENVELPLTYRGMRASDRKRNVQEALEKVGMSHRTKHYPAQLSGGQQQRVAVA
                     RALAGGPSILLADEPTGNLDSANGQAVMDLLSDLHRQGATICMVTHDPRYALCADRSV
                     HLFDGRVVEESVEAGR"
     gene            21323..23731
                     /locus_tag="VLE22_10710"
     CDS             21323..23731
                     /locus_tag="VLE22_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14920.1"
                     /translation="MSREIRHAVRALAKSPGFSAAVVLTLSLGIGANTAVFSVVNAVL
                     LRPLPFEDPERLALIYESRPRIGSERSSSSEANYFGWRERCRSLERISAFNLHFFTLT
                     GVESPEEIQGAIVSADFFPLLGVKPYAGRTFSADEDRPGNNQVVLLSYELWQRQFGSD
                     PGVIGRKLILNEKSFSVIGIMPPHFSFPPVGQAGLWAPISLDASPPEQFARRWYLRVL
                     ARLKPGVTFEKAQVELSAVSAGLERQYPEANAGFRAHLAPLHGELVRSVRTSVLLLWG
                     AVGFVLLIACANVANLLLARAARRKREIAIRIALGAGPWNRVSQTLSETVVLALSGGV
                     IGVFFAWWGVDLLSRLGLEAVPSSGEIGVDVKVLTFTLGISLVTGIILALFPVWNSRI
                     PDIPGALREGGSRSRDERNPSRARWILIVSELALSLVLLTGAGLMLESLRRLSATDPG
                     FETGNILTMSLSHPRSRFGPLKGRADFFAQLLERIEHLPGVRAVGAVNALPLSGSDSS
                     SSFVIEGRPAQPHGRFTSAQIRIITPGYLRAMGIPLVAGRGFTHADAANTPRVAIINR
                     AMSRWQFRGENPLGRRLAFDTSGPFREIVGVVGDVRHERLGVEPVPEIYLPHAQSPLA
                     SMMLAVRTTDEPSMVTPAIRAEVHALDKDQPVTGVATLDERLERSMSRSRFDAVVLGL
                     FAALSCALAVVGVYGLVNYSVAQRTHEFGVRLALGADKGEVVWTALREGLGLTAAGIG
                     IGLVGAIALTSVLSSLLYSVRPRDPAVLLAAGLLLAATSMLATWLPARRAAKVDPMKA
                     LRYE"
     gene            23741..26218
                     /locus_tag="VLE22_10715"
     CDS             23741..26218
                     /locus_tag="VLE22_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB14921.1"
                     /translation="MGIVSDFRIALRMLARSPGFVVVSVLSLALGIGVNVSVFSSVNA
                     LFLRPLPAVPESGRLVSIYHRTKSGSFTSSSYPDYTYYREQNHVFSGMAAYLRFPTNL
                     RSGEQTERIYGELVSSDYFSVLGLSPALGRLIVPDEREPVAVLSHDFWQRRFGGNRGV
                     IGQAVRLGSGLFTVVGVAPREFRGIVLDWAESPSVWLPVYTYKEALPTPPFGTVDFLH
                     YWGNQSYELVGRLRDGVTLGQAAAEMTALAKRIQKDHPERARVWSKEMAEYGDLDAVV
                     FPTNRMRLWPGDRSNLIDFLVLLAAVAGLVLLIACLNVASLVLARTTKRRRELAIRLS
                     LGAGRWTIVRQLLAENLLLSLFGGAIGILVSGWTSTFLASFHQAFRLPVPPETAVDVR
                     VLAAATGVSVVSAALLVLFPARLAFRLDVNTGLKTDMGTTSASGLRAHHALVLGQVAL
                     SVVMVVAAGLFVRTLWNAQAEDFTSQSENVLLGSLDLGSRGYDGDRGGRVYSQVLERV
                     RTVPGVRDAALVFVVPLGGRRGGTNVVITPPDGGVKRSVQIGLNVVTPGYFQMIGIPV
                     VRGRDFSDADRSGAPAVAMINDVMAQTLFPGREPVGQRFLLTWPPAATVEVIGVVRDG
                     KFRNYRSEREPTVYVPLAQRFMMAMNLEVRTAGNPSALAAAINREVAAVDKDLPLNAF
                     QTLKTHFETALARERLTAALLSALGALALVLTAIGLYGVLSYAVSQRTREIGIRMALG
                     ARSQSVVRAVLGSTMRIVTAGLGIGVAVALALSSVVRGLLYGITPTDPAVFATTILVL
                     AGTAALASYLPARRAAKVDPMKALRYE"
     gene            26232..26912
                     /locus_tag="VLE22_10720"
     CDS             26232..26912
                     /locus_tag="VLE22_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB14922.1"
                     /translation="MIQLNNIEKHYDVGPNKFFVLRRITMEVKEGEFVTIMGPSGAGK
                     STLLSIVGMLDGAWSGEYHFFDQPVHALKPKQRIELNKKYIGFVFQSYHLLDDLTVYE
                     NLDLPLSYRNLKGSERAGIVCDTLDRFQIVGKKDLYPSQLSGGQQQLVAVARAVVAKP
                     KLILADEPTGNLHSEQAQEIMELFKKLNDVGTTIIQVTHSERNAAYGNRTIYLEDGWI
                     AKDRPSGY"
     gene            26965..28335
                     /locus_tag="VLE22_10725"
     CDS             26965..28335
                     /locus_tag="VLE22_10725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB14923.1"
                     /translation="MKPSDAVTPRILVADDQPDVLEALRLLLKPEGYRIETASSPASI
                     IAALESQDFDVVLMDLNYARDTTSGQEGLDLLHRIQAMDKTLPVVLMTAWGSIELAVE
                     GMRRGARDFIQKPWDNARLLAILRTQIELARALRQGERLEAENRVLRGDGAPVMIADS
                     PAMQPVLQVIARVGPSDANVLITGESGVGKGLIARALHAASLRAVRPFVTVNTGGLSE
                     SLFESELFGHVKGAFTDAKADRVGRFELAENGTLFLDEIANVPLSQQAKLLRAIETGE
                     FERVGSSRTRRVDLRLISATNADLRAEVAAGRFREDLLFRLNTVEIPIPPLRQRREDV
                     GPLATYFLRQHCQRYRKCLGAFEPAAMQLLLSHTWPGNVRELDHAVERAVLMAQGEAI
                     RATDLALRQPREGSGRIEDMSLEDVEAFLIKKALARFGGNVTQAAHTLGLSRSALYRR
                     LQRYGL"
     gene            28332..29669
                     /locus_tag="VLE22_10730"
     CDS             28332..29669
                     /locus_tag="VLE22_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB14924.1"
                     /translation="MSAGGRRTRSRFAYETRLTLLALLTGTPALVAAMILLWTGGFTP
                     KVQWTVTIVVVCFWLGAAFALRERVVRPLQTLSNMLAALREEDYSMRARSGRSDDALG
                     EVFVEANALGQMLREQRLGAMEATALLRTVMVEIDVAVFTFDGEQKLRLVNRAGERLL
                     GRPADRLLGRTAEELALAGCLDGEPVRAIELALPGASGRWGVRVSTFREKGLPHRLLV
                     LADLTRTLREEELQAWKRLVRVLGHELNNSLTPIKSIAGSLRTLLEQDQRPPDWKEDT
                     QRGLDVIAARAEALSRFMEAYARLAKLPPPKLGSVDVEAWVRRVAGLETRLPVTVEEG
                     PAILIRGDGDQLDQLLINLIRNAADASLETHGGVRTGWRRIGGHLEIWVQDDGPGISN
                     TSNLFVPFFTTKASGSGIGLVLSRQIAEAHGGVLALENRSPGPGCEARLRLPM"
     gene            29703..31550
                     /locus_tag="VLE22_10735"
     CDS             29703..31550
                     /locus_tag="VLE22_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HSB14925.1"
                     /translation="MRLVHLMLCFIVLLAVSCRRTPSNERPKDSHSYSNPELVRVRHV
                     DLDLEAFFDQKTLRGTAVLELERAKDGESAPLILDTRDLRIEGVEGSLDGTDWSPVKF
                     EAGAMDPILGAPLTIHLPAGGKHVRIGYMTSPNATALQWLEPAQTAGKKLPFLYTQSE
                     AIHARSWIPLQDTPGVRVTYSARIRAAKGLRAVMSAEHVETGAEGEFRFRMAQPIPSY
                     LIALAVGDLAFRPLGSRTGVYAEPPVVDAAAREFEDTEKMMQAAEELYGPYRWGRYDL
                     LILPPSFPYGGMENPRLTFTSPTVIAGDKSLVSLVAHELAHSWSGNLVTNATWDHFWL
                     NEGFTVYVEQRIIEKIYGKERAEMEAVLATESLKREMERLEPRDQALQLDLRGRDPDA
                     GSTDVAYVKGALFLRTIERAAGRPAFDAFLRSYFDRFAFRSVTTKDFLDCLAKNLKTN
                     VPVEEWVHKPGLPSGAALPVSDALAKVSDLAGQWAQGMIPSRTIPYAKWSTQERIQFL
                     QALPADPGGRRMQALDQAFGITRSGNAEVLQPWLLMSIRNGYTPASAKLEEFLMRVGR
                     MRYIRPLYEELAKTPEGMERARAIFAKAQGGYHPITATAIEAALQRPNR"
     gene            31660..>32007
                     /locus_tag="VLE22_10740"
     CDS             31660..>32007
                     /locus_tag="VLE22_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14926.1"
                     /translation="MRRILGNVLALSVSAVAILAGAPRTATEKPGPPPKSGFYSFWNI
                     GEEFGEGIGNVSKLFCEIGTATQPPIYTGNMLIDCDGEVPHNETSIAVNPNDPKHVVG
                     AYHSYQVKFRGATA"
BASE COUNT         5997 a         9243 c        10916 g         5851 t
ORIGIN      
        1 ctccagatgt gcgtgccgag ccatgttgaa tagcgccgcc atatcctcga acatggttgc
       61 ctcaagggca cggatttccc agcccccagg gtatttctta ccaatgcaat ggggatcaat
      121 gctaactcgc caatgtggat cgagtcccaa gctgtcatat ctcgaaagcc attcagatag
      181 acgccagaca ggaatgtagc aggcataatc gcttggggcg gagttctgaa ggtcatccat
      241 tagcgccagc agtttaccct ggtccaattt gatccccaaa tcatcgcgca tcttttccac
      301 gttcctccgc agcatctcgg cgaattccct caactgcttc gttggctgcg ttgccaaagc
      361 cgcgacgtgt gctcgacgat cttctgaatt tatccgttgg gggagattgg ccaggattgc
      421 gatagcacac tgctcgttga acagagccgt ggcttttcgc agtagcaggt ctcttccgga
      481 gaccccaatg gatcgtggtg ccttcatttt cattatgcgt ggatcttagc aggattcgcg
      541 gcccgacacg cttgcaccgg gagtgggcac cctgcgctcg cacttcgcgt cgaccttggg
      601 cttcgtcaac accctcgcag cccgcgcgcg cgcccacagg acctcgcccc gtgcgccgct
      661 ctctgccctc acgcttgcgc cgcttgccct gccaccgccc gctgaagcgg aagagtcgcg
      721 ctgcgccgcg ctcgcttcgc ttgcaccacc tacactaccc ttttggggcg gggtggggat
      781 gctcaatatc ggatgtgaat gtccgaaatg tagcagtttt cccgctcgta tagctctccc
      841 caaagagtaa ggctgcagcc cagtggggta ggtgtatact ggggccaact gggagaccga
      901 aatgaattcc ggtcaagccc ttttgccgtg tagcgagtaa agatccactt aactggagga
      961 agtccatgtc caaagttgca accgttgcag tctgtttgtc cttgctcacg tttccagcgt
     1021 ttgccgacgg agaggccaac tgcgcgggcc ttcccaccgc cagagacttg aataagctgc
     1081 ttgtcgcggc cgccggcgac gacatcggcg gcctctttaa tggtgagcgc atgtgggccg
     1141 ccgtggtcaa ccgttcgggg gaaatctgcg cgtacgcgac atcgacttcc gatccgaccc
     1201 aagtttggcc cggcagccag gcgatcgcca agtcaaaggc atacacggca aacgccttca
     1261 gccttgacac gctggcgttg tcgaccgccc ggctgtacac gttcacccag ccgggacact
     1321 cgctgtggag cctgggccaa tcgaacccct tcgagccaaa gtggctcgct ccgcccagag
     1381 gcaattcggg aggcaggaac cagctcgccg gcggcctgat cttcttcggc ggcggcttgc
     1441 cgctgtacca gggggggaag atcatcggcg gcctgggcgt gagcggcgac acgagttgcg
     1501 ccgatcatga agttgccaag aaggtgcggg atgccgcggg cctgaatcct ccgggaggaa
     1561 agctggctga cgacatcacg tattcttccg cagatggcgc ctcggcgttc acgcatcccc
     1621 tctgccccaa caccaagcgc aacgggacgt tccttggcaa cgaggcgccg gcgtcgggct
     1681 actagcgtat tgcgttgtcg ggcagacgat cggcttctgt cgtctgccgc cctcgcagga
     1741 cacgaccaag gcacggcggc acaaagaagt attctgaaaa agtgaacttc cgaacgctcg
     1801 cgatccatgc gggtcagggc cccgatcccg cctatggcgc cgtcatgccg ccgatttatc
     1861 agacgtcgac gttcgcgttt cgcggcgtca acgagccggg cccgtttgat tactcaagga
     1921 gcgggaatcc gacccggaaa gctctggaga cctgcctcgc caggttggag ggcggagtcg
     1981 cgggcttcgc gttcggaacg gggatggccg cggagacgac ggtgttgatg ttgttcgatg
     2041 ccggggaccg gatcatcgtc cacaacgacc tctatggcgg cacgtaccgg ctgctgacga
     2101 ccgtattggc aaacaagggg atcggggtcg atttcgtcga tcttcgccgt tcggatctgg
     2161 ttcgtcaagc tatccggccg gagacaagag ccatatggat tgaaacaccg accaatccgc
     2221 tgatgaacct ggtggacatg gccgaggtcg caggcatcgc ccgcgagaac ggattgctga
     2281 ccatctgcga caacaccttc ctgtcgccct atttccagcg cccattcgag tttggcatcg
     2341 acatcgtggt tcactcgacg accaagtaca tcaacgggca ttcggacgtg gtcggcggcg
     2401 cggtggtggt aaacaacgat gcgctggccg agcgcatcgg ctatcttcag aacgcgctgg
     2461 gcacctgcgc ggggccgcag gattgctttc tggtcctgcg cggcatcaag acgctggcgg
     2521 taagaatgga ggagcacaac cgcaacgcgc tcgaaatcgc gcactggctc gaacagcaca
     2581 aaaagattgc ggccgtgctg catccgggtc tgccgagcca cccccagcat gatctggccc
     2641 tgcggcagat gcgcggtttt ggcggaacct tctcgtttcg cgtcaccggt ggcaggaccg
     2701 aggttttccg gctgctgggc ggagtccggc tgttcacgtt ggccgagtcg ctgggcggcg
     2761 tcgaatccct gatcgagcac cccgccacca tgacgcacgc atcggtgccg caggaagagc
     2821 gcgagcgcat gggcatcacc gacaacgtgg tgcgggtttc ggttggtatt gaggatgtcc
     2881 aggatctggt cgccgatctg gatcagtccc tgtcgagagt ttagcgtggg atggcggact
     2941 tgccacggag acacggatgc tcagaggcct gattcacggc attttaggca agtttggcta
     3001 cgacgtgatc agcacgcgcg ggcgatacgc aagcgacggg ctgtttaccc tgcacagcga
     3061 ccgattccgg gaggatccgg cgtttcgacg cgcgtatgcc cggggcgtgc gagccggctg
     3121 gggctacgat ccccggttca cgtggcgcgt ccatgttgcg ctctgggctg cgaaggcggc
     3181 attacgtgtg ccgggcgact tcgttgaatg cggcgtcaat gccggcttca tcagctcggc
     3241 catcatggag tatctcgact ggcgcaacgt ggacaagaag ttctacctag ttgatacctt
     3301 taccggccct gtcttcttgc agtactccgc ggcggaggtg gctgccggga gggtggaggc
     3361 cgcgcgcaac gctctggcgg ccggcgccta cgtgacggac ctcgagcgag tccaagcgaa
     3421 cttcgccgag tggccgagcg caatcgtcgt ccagggaact gtgcctgacg ttctgccgcg
     3481 cttgccggtc agcagcgtcg cgttgctcca catcgacttg aactgcgcgg gccccgaacg
     3541 cgcggcgctt gagtatttct gggaccggtt atcgcctggt gctctggttc ttttcgacga
     3601 ttacacctac tttgggcagc aatgccaggg cgacgccatc gatgaggccg cgcgacagct
     3661 cggcgccacc gtgttatcac tacctacagg tcaaggcttg atcatcaagt agcttcgtca
     3721 ttggcacccg gcctcgattt catctcaaaa gctgagtcgc agacccaact ggacggttcg
     3781 cggaggccgc gcgctcagaa tcgtgccgaa gtcggctgcg ccgagggtgt gcccggggag
     3841 atcgaagttg gcgtggttga atgcgttgta gaactcgccc cggagatcca cccgatatcg
     3901 ttcggtcacc tcgaactcct tggctaccgt caggtccaca gactgctgaa acggccctcg
     3961 aagaaccgac cgcggagcgt tgccaaatcg aaattgagcg ggattgcgaa acgcgtcggt
     4021 attgaaccag cgttcgagtg accgctgcgc ccccggcagt tctgcagcac cgatgaggtc
     4081 ggggcgcagg ggaccggcgg aaaacgcgtt cgtggtattc gccagcgtcg tcaccgtaaa
     4141 cggagcgccg gactgccagg taccgaaacc gccgagcttc cagccgccgc caacaatatt
     4201 cagatagcga ttggacctga tgcgaggcac ttcgtacagg atgctgagga cggtacggtg
     4261 tggaacatcg gtgccgctaa gcgacttatc gagccgccgg ttgtaggcgt ccatgtaact
     4321 ctgcggatca ccgtattcgt tcgatgacac tacgtcgtcc agaaacttcg agaacgtgta
     4381 gtgcgccaga aacgagaggc cgcgcgcgaa gcgcttctcg gcccgtatgt atcccgcgtg
     4441 ataggtcgag ttcccaatcg cggggttgat gatatagacg ttgctgaact gggggaacgg
     4501 cctgcgggcc tgggcatcgc caggtcccat gagctccggt gcgacctggt tgaggctgag
     4561 gtcgttggcc gtgagatggt gacttacgtt ggctatgtag ccggcttcca cgacggtttc
     4621 gctcgccacc acgcgctgga tattcgcgtt gtattgatac gagaccggcg tcggccggtt
     4681 acgctcgaaa aaaccaaccg cagtgtacgg tctccggccc agcggcacgg ctccgaaccg
     4741 ggcatcgagc gggggacgct cgaacgcggg gaagccgttg cgaagctgca tggcgctcag
     4801 caggtcggcc tgcggcacga cgagcgatgc agcggtggag aaaccggtcg aggcggtgtc
     4861 gccaatcgta ttgctgacgg tggatccgta aaagacgccg gcggcggcgc ggatcacggt
     4921 ctcagactgg aagggcaagc ggtaggcgaa cccgacccgc ggcccgaagt tgttccagtc
     4981 cgtgttgaaa gcctgccgag gcacgccgtc gcggccggag aacgttacga ttccgggagt
     5041 gccggagacg ggattgatcg cgctcagatc gaacgaattc tgccggtctt cgtcgactcg
     5101 ccggggaagt tcggactccc agcggagacc gaagttcaag gtcaggtgat cggtcgcacg
     5161 ccagtcgtcc tgaacgtacc ccgccaagta gtacgcccgc gacgtaattt gatcggaaac
     5221 gttgacgctt gccgagttca cctcgcccaa cagaaagctg gcgaaagcgt cgccggcgcc
     5281 ggacgttccc ggcttgccgg tgatgagcgg agtaatgccg aatagaccgg ccgaggaccg
     5341 gtcgcgtact tcggtattgc ggccgcgccg gtgctcgaca ccaaacttca gcgcatgcct
     5401 gccgcggaac caggagacgg cctccaggat ctgggtatcg cggatcggcg tctggtggcg
     5461 ccccacctgg ccgcccaagg atgcatagcc gggaatcgtg aaagtcggaa acgcggcggc
     5521 gctcacaccg gtgagaccga tggctcccgc caggttctcg ccgaacccgt aacgttcatc
     5581 gataaacctg cgctggaaga agctgacttt caactcgctg atgagattcg ggctgaaggt
     5641 gtgggtgtgg cccgccagaa cactctgaat gcgcacgtcg ttcacgttcg cgtcgggcgc
     5701 agcttcacgc cgcgggaagg aaccgcggtt cccgataaag ctgtcgttca ggtagtaacg
     5761 gacagtcagc tgatcgttcg agcgcagttg gtggtcaact ttcgcgacga aaatgttgcg
     5821 gtgaaggtcc gagtcggcgt tggcggcgta gttgctgccg ccggccggcc cgggcgtgcg
     5881 attcggcgag ggccagtatg acaatgccgc cagggccacg ggatcgaagc ggtcgagggg
     5941 tatgcggttc tcggggaatg gctgcctttc gcggccaacg gtggtcgagg gatcgtagat
     6001 cgggatcacg cgtccggcgc cgtcgcgcaa gccgaagaag tcgcccgccc gttgggcgag
     6061 atccggcacc gtctgaaggt cgatcgtcga ggtgagctgg cgggtttgtt cccaggtggc
     6121 gaagaagtgc gtcttgtcct ttcggatcgg cccgccgagg gagccaccga actgatgcat
     6181 gcggatgggc gctctctcgg cggcgaaaaa attccgcgcg tcgagagcgc tgttgcggag
     6241 gaattcgaag acgctgccgt gaagctggtt cgtgccggag cgggtggaca gggtgatgat
     6301 gccgccggtc gagtgcccgt attcggccga gtaggcgttc ccaatgacgc ggaactcctg
     6361 cattgcgtcc atcggcaggc tggtcatttg ttgaggccgg gtcagcccga cggcgttggt
     6421 gacgttgccg ccgtccaacg tgaagttctg gttgcgcgcg cggccgcctg cgacgctgaa
     6481 gacagggtag ttttccgctc cttgtccggt gtcgatcatc acgacgcccg gggccagcgc
     6541 gacaagtgaa gtcgccgcgc ggttcggcat gggaaggccg gagaccattt tccgattcac
     6601 gagttgcccc acactggagg actgcgtctc tgcgaccggc acggtctcgg tgacagtgac
     6661 ggtctcggag gcctggccga cctggagtgt cagatcgacc ggcagaacct ggctgaccgc
     6721 caatacgagg cccccgcgag atgctttctc gaagcccgcc gcggtcgctt cgatgcggta
     6781 ccttgccggc agcagcgagg ccaggcgata gtttccggct tcgttggttc tagtcacgaa
     6841 gcgggctccc gattcctcgt tcaccagcac gatccccgca tttgggattg cactcgatgt
     6901 ggcgtccgta acggtgccgg tgatggagct tgttccggtt tgggcgtaca ggagagccga
     6961 accggcaagg aataagacca acttccgaaa tctgtgaagg aactgcatat ttcccctctc
     7021 ttcgcgcaca ctacgaccgc aacttcattt cgctgcggat cggtggtccg gaaccggccg
     7081 gacgcagaat ggaacggatc agatcaacaa aaggtgggca atcagcaaca acagcggctg
     7141 cgtccgccgg cggtggggta gatgactgag tgaatggttc tcatcgaagc tttcctcaag
     7201 aatacgcatc cgcagggtgc gtgtcaagga aaatctgcat attgtcgatg gagattgagt
     7261 agatcattta ctggctgaat ataaacgggt tattaatctc accacggaga cacggagggc
     7321 acaaagggag gaggcgaggg tcaccggcta aagccggtgc caccttgaag gtgcaccttg
     7381 tggacgctgc cagcgataga atggaggccg catggaacgg agaacactgt tgaaggcggc
     7441 ggcgctgctt ggagcgccag cggcgctgcc cggggaagat ttgtatcggc gagatcctga
     7501 gaagtactgg cgtcggattc gcgaggaaca gttcctgttg cccgggtggc gctcgttcct
     7561 caacaacggt agtctcggta tcgcgcccag gccggtgttg aaggcggtga acgactatct
     7621 cgaacggtcg gcggcgctga tggtggagga atacccgcgc tggggctacg agacgctcga
     7681 tgagcatcgc caggaactgg ccgagttctt cggctgcaaa accgaggaac tcgcgctcac
     7741 acacaacgcg accgaggcga tgaacatcgt cgccaacgga ctcgacctga aagaaggcga
     7801 cgaggttctc agcaccgacc aggaacatcc gggcggcttg tgctgctggc tccagcgaca
     7861 ggcgcggcat ggcatgaccg tgcggcaggt ggcgatccca ctgcctccca aaaaccaggg
     7921 gcagcttgcc gacctgctga tcggatcgat cggtccgcgc acgcgtgtgc tcagcttcag
     7981 cggtatcacc acgatgaccg gactgatctt cccgatccgg gacatctgcc tggccgcgcg
     8041 ggctaagggc gtcatcacgg tggtggacgg ggctcacatg cagggtcaga ttccgttccg
     8101 gctcgacgaa cttggctgcg atttcctcgc ggccagcccg cacaaatggc tgtacacgcc
     8161 ggccgggtgc ggcttcctct acggacgcga ggagatgctc gaccggctgt gggtcaacgt
     8221 ggcgaccgcc ggatgggaca agaaagaact gaaagccgcg cgattcatga tggtcggaac
     8281 aaacaatcgg gccatcttcg aggggtacat cgccgggttg cggtttctga aacagctcgg
     8341 gccggagcgc gtgtacacgc gcatccacca gcttggtcgg atgtgcttcg agaaggcgcg
     8401 tgggacgcct ggcgttgaga tgctgacgcc ggacgacgac cttatgttcg cgggcttagt
     8461 gacgatgaag ctgaaggacg gccactggaa acgagtgcag gagttctgtg cgaagaaacg
     8521 gatctgggtg cttcaatcca gccggagccg gatctcgacg cacattcaca cgcgcccgca
     8581 ggacctggat gagttcttcg agatcctgcg aggagtcacg gcggcctagg atcaccggct
     8641 ggagccagcg ccactggtga gatggcgatc gaagaagccg gcggcagcct ggagggcttc
     8701 gagccagcgg gcgtgcgtca ggaagccgtg gatttcgtcg ggaaagacga gttgctcaaa
     8761 gtccacttcc tgcttccgca gcgcttcggc cagagcaacc gtctggcgga actcgacgtt
     8821 gcgatcgtcg tcgccgtgga tcagcagaac cggcgagcgc cagccgctca tgtgcgccag
     8881 cggcgacgac tcgaaggcca cgcggagcgc ccgctgctgc gtgtcgagat catcggatga
     8941 gacgtatacg cgggtctcgg ttcgccagtc gtggacgccg tggaggtcca caccggcggc
     9001 aaacagatcc gaggcgcgag cgaggcccag cgcggtgagg tagcccccat aagagccgcc
     9061 ccatagcccg atgcgggcgg ggtccacgtc tggacggttg cggaggtaga ggcccgcgcc
     9121 aaggacgtcg ttgaactcgc tcgcacccgc ggctccgtag ttcagcgcct cacggaactc
     9181 caggccgtaa ccggtgccgc ttcggtagtt gaccgaaagg acgacgtagc cgcgactagc
     9241 aagatactga ttgagcgcgt aggtgcgatg gtagtagtcc atgtaatgcc cgccgagaag
     9301 catctggcgg cgggatccac cgtggaaaaa gatgacggcg ggccggcgct ctccctggcg
     9361 gactcccgac ggtaggaaga gctggccgcg gatcttcatg ccgtccgcgg cggggaactc
     9421 gataggctcg ggaatgacaa gtgcgccggg gaagccggag gggatggttt ctggggcgag
     9481 gtcgcggggg ccatcttcgt tcagcacggc ggcacgagcg gggcggcgcg cgtcggaacg
     9541 gaaaaaggcg accgtcgcgc cgccgcccag gaggaccgga gaccattcga ttccggagcc
     9601 tgatgtgatg gcgcgcgggc ggccgcctgc gaccggaaca cgccagaggt ggcgccggtc
     9661 gatgtcgtcc tggttcgagg aataaatgac ctcgctgcgg tccgggctga cgactacgtg
     9721 ctcgacctcg aaagcgcccg aggtgagcgg aagcgtatca cctccggaga ccgggaccga
     9781 atagaggtgg gtccagccgc cggcttccca tggaaacacg atgcggccgc cagcagccca
     9841 aaggagctgg ttttccgcga tgacgccgtg gaagacgctg cccgcgccag gcttcgcgcg
     9901 ccagatctcc cggccgcggc cattcgcgac atcggcgacg cggacggacc atggctcggc
     9961 gctgcgcttg gggccgaaga tggcgagcac gcgcgagttg gggacgcgga ggaaggcgat
    10021 cgagcggccg tcgggggacc aggcaggctc gctgtcgagg tcgaggcccg ggtcgaggta
    10081 gcggagggtc ttcgcggcgg gatcatagac gccgatgaag ctgtggtctg cgcgggcgct
    10141 gacgaaggcg agcaagacgc ctccgggaga ccatcggagg gagccgcact ggccgcgggc
    10201 acggatgagt tgcgcgggct tttcgtcgcc ggcaagaggc gcggaccaga tctgtccttt
    10261 cttgaggtag gcgatgcggt cgcctttggg cgagacggcc ggggcggagc cgtcgtcgat
    10321 gcggcgtggc tcgccgccgg agagcggcac gatccagagg gcttgttcag cgccagccgg
    10381 gtcgctcaaa gggttcggat tctcgccctg cgcgttggcg gagccgccgc ggacgaagat
    10441 gagcgagcgc gcatcgggcg tccatgccac accggagatt tcctggccgt cgtcggagga
    10501 gtaggatgtg acaggacggg atttgtacgc aggcggttca gccacccaga tgttgcgcac
    10561 gccacgggca ttgagcaccc aggccacggc cccacccgag ggcgcggcgg tgagttccga
    10621 cggaaatggc gaactcagcg cctgctccag ggtgaaaggt tcctgcgcga aggcagcggg
    10681 agacagaaga agcgcgagca acgcggcaga gtgacgcatg agagcctcct atttttgtga
    10741 gtgtagtcca aggacagaaa tctgggcgga ttctgcgaag atcagagttt ggaggataga
    10801 ggatggacga gcggcaagag gcgattgttc ggaaggcgtt ggactcattt cagattgaga
    10861 taccgtcgtg ggggtttgcg aacacgggca cccgattcgg caagttcgtg caggcggaag
    10921 cggccactac aatcgaggag aagctcagcg acgcgggaca ggtccaccag tttacgagct
    10981 gctgtccgag cgtagcggtt catgtgctgt gggatttccc ggaaggccgc aagagcgttc
    11041 cggaggcgat ggcgctggcg gcgaaatacg gcgtgcggat cggcgcgatc aatccaaacg
    11101 tattccagga ccagatctac aagcacgggt cgctcggcag tccggatccg gaagctcgcg
    11161 cgatggcgct ggcgcacatt ctggattgcg tggagatcgc gaaggaaaca ggtagccggg
    11221 atctgtcgct atggtttatc gacggttcga gctatccggg cacggccaat attcgtcacc
    11281 gcaagcaatg gttcgcggat gggcttcgcg cggcgcatga gcggctggct gcgaaccagc
    11341 ggatgctggt ggagtacaag ccattcgagc cggccttcta ccacaccgac atcgccgact
    11401 ggggcatggc gctgctgcta gcgcgggcgg cgggaccgca ggcgcgggtg ctggtggaca
    11461 cgggtcacca ctaccaggca cagaacatcg agcagatcgt ggcctggctg ttgagcgaag
    11521 ggatgctcgg cgggttccac ttcaacgacc gccggtatgc cgacgacgac cttacgatcg
    11581 gttcaatcga tccgtaccag gtgttccgta tcttccacga gattcaattc cacgaatggg
    11641 agacggggaa gcgcggcgac atcgcgtaca tgatcgacca gagccacaat ctgaagcaga
    11701 agatcgagga gatgatccag acggcatcaa cggcgcagga actgtgggcg aaagcgtcgc
    11761 tggtggatca cgaagcactg gcccggagcc aggcgaagtg cgagctgatc gacgccgagg
    11821 aatatctcaa ggctgcgttc gctaccgatg tgcggccggc gctgcgggcg tggcgaaagt
    11881 cgaaagggtt ggctgaggaa ccactggcag cgtttcgcgc gagcgggtac ttggagcggg
    11941 cggcacgcga gcgcggcgag cgaaaccggg gcgcggtggc aagctacgcg taaggagagc
    12001 gagacactgg agggatttat ggatgcgctc gtaaggcatg agggcgaggc gccgcgggaa
    12061 cgatcgacct gcggctggcg gcacctgctg atcagcagac aggaccgcgg acgggcggcg
    12121 gcgtgggcgc acgcagtgga tattgacggc gcacgcgagc actaccacaa gcaggggacg
    12181 gaattgtatt acgtgctcga gggcgaagga tcagtggtgc tggacggggt ggagcatccg
    12241 gtgcgaaagg gttcgctcgt ccagatcccg ccgggcgtgg tgcatggcgc gcgggggcga
    12301 atgagagtgc ttgtgatcgg catcccggat atcagcgacg aagacctgta ttttccggaa
    12361 gacgcggcag agcacggtcc aaccgtgcag ggtgactgat ggcgggccga cgacggaaaa
    12421 gcggaagagc ctgcgccatc gtggtgttgc tgtgctgcgc gccggctgca tggccggagg
    12481 aggaaatcgc ggagggcgtc gcgccgcggg tgcggccgct ctctgcatca gacacggcta
    12541 cggcgacgcc gacgagcgaa ccgtggatcg actcgaacct gtggctggtg cggtcgctga
    12601 ccgggcacag ccgcgcgccg gtttggctgc actttcgcgt ggacgggggt tccggagacg
    12661 actacgcccg cgccatcgcg gatacggccg cggctggggg ccgctgggcc gtcgaactgg
    12721 atgacggctt tcaggcggat ctgcggagcg gacagtcgga ggcgctcgcg aagtggggac
    12781 ggatcgtcaa ctatctgcga tttttcgaag atcaccgggg gtggcgggag tttgtgccgt
    12841 ccggccctct cggcatcgtg tacgatcccg ccgggaagta cgtcgacatt tcgaatgagt
    12901 atctcaacct gatcgcgcgg cggcggattc cctaccgggt gatccaccgg gcggacttgg
    12961 gtgccggcgc cctgggagga ttgaaggccg tgctggcaat cgacgcgtcg ccgcccagga
    13021 ctggggaacg cgcgttgatg gaatcgttcg ccgaggcagg cggattggtc gtgacgggtc
    13081 cgggctgggg gaaagcgccg gcgggcggcg agggttacga agtcgaaaat tccggcaagg
    13141 ggcgggtggc gatctaccga gaggatccgc cggacccgga aaccgtctcc agggacatgc
    13201 tcacgctgat cggacgggcg aacaccggca tccggctctt caacgggccg tcggtgctct
    13261 gccacgtgac gacgggcgag gcaggggcaa gactgctggt gcagttgatc aactatgcga
    13321 cgcttccggc cgaagcggta tcattgcggg tcgatggcga gtacaaggcg gcgcgtgtgt
    13381 tgagcccgga tgcccctccc gcggagctgg cggtggaatt ggcaggagga cgcacgcagg
    13441 tgtcgctgcc tgccttttcg gtgtatgctg cggtagtgtt cgagaagtag ttggatggag
    13501 gactcatgaa ggacggatgt aatgggttga cgcggcggca gtggcttgcg ggagccggag
    13561 tagcgctggg cgcggcgtcg ctcgggcgtg ggcaggggcc ggcttcggtc ccgccggtat
    13621 cgatcgggaa gtacgccagc tatgacgacg atctcgtggg gcggctcgcg aagcagttcg
    13681 accaggcggg cggactcggc gggttggtgc gagggaaaac agtcaccgtc aagctgaacc
    13741 tgaccggcgg cggcaggttt cccggatgca cgccggggca gacccactgg gtgcacccaa
    13801 agctggtagg cgcctgctgc tacctgttcg gaaaggcggg agcgaaacgg gtccggctgg
    13861 tggaggggac ctacgaggga gagtctctcc aggacaagat gctggacggt ggctgggacg
    13921 tgaaggcgat ccagtccgcg gcaccattgg tggagttcga ggaaacaaac acgctcggct
    13981 cgggcaagcg gtattcgcga ctcagggtag ccaagccata tattttcccg gcgttcgact
    14041 tgaaccatac ctacgaggac acagacgtat tcgtcaactt gtcgaagctg aagcagcacg
    14101 aagagtgcgg gatcactctg tcgatcaaga acatgttcgg cgtcgcgccg aactcgattt
    14161 acggcgatga cgcgggcgtt gatgagccga acgagaaccc gcgaaagggc cgggagcatg
    14221 tgttgcacct ggggaggcgc cagccgtcga agagcgcgcc cggggaagtc aatccggcat
    14281 catcgcgata cgagggacac cgggtgccac gaatctgcgt ggacctttcg gcggcgaggc
    14341 cgactgatct ggccatcgtc gacggaatcg agacgtgcgt gggcggcgag ggaccgtggg
    14401 tgaaaggctc gaagcatgtg cagccgggag tggtgatcct tgggaaaaac gccgtgtcaa
    14461 cggatacggt ggcgacagcg gtaatgggct tcgatccgcg ggcgaagcgc ggcgagggac
    14521 cgttccaggt cgactacaag agccaccccc agatgccggg cgacccgaag caggccgaca
    14581 atccgatgct gctggcggag gcggccggac tcggcagcgc ggacctgagc cggatcgagg
    14641 tggcaggcgt atcgattaag gacgccctgt tcgacttcga gaagcaccgg aagggctgaa
    14701 ccgaagaatc ggcgggtggc cggtccgccg tttacgcgtc ccagtccttg ttccactcga
    14761 cgcggcgctt ttccttgtag gcgatgttgc cgatgtggca agccatcgcc gagtagtggc
    14821 cctcgaccac atcggcgttc ggttgcttgc ggctgcggat gcagtcgagc cagtttttca
    14881 tgtggggaac ctccgcggta ccgccagcgg cgatctcgcc cacttcgctg ttcttcgtcg
    14941 ccggaatgta ccggtagccg taccgaaaga tgtggaagcg gcctccggta cccataaaga
    15001 cgatgtccgc cgaatcgcgc gggatcatgt cggtgatggt ggcctcgaag gtgacggtga
    15061 gcttctcggg gtaatccagg atgaagttca cgttgtcggg cgtgtcgcgc ccatcatcga
    15121 actggtagat tccgccaagg gcgacggcgg agaccggttt ccggatgttg aggaaccagt
    15181 gaaccacgtc caccatgtgg acgaagaggc cgcccgtctg gccgccggtg gcgaaatccc
    15241 agtaggcgaa gcgattgaag tagcgcttcg ggtcccaggg aatcttcggc agccagccga
    15301 ggacggcgtc ccaatcgaga ccggcgggct tctgctccat gccgggcggc accggggtca
    15361 agtatccgcc gttggcgttc cagatggtgc ggaccatgtt gacctggccc accaggccgc
    15421 tgtcgaagaa cttctgcttc gcctcgatga agtgggcgag actgcgctgc tggacgccaa
    15481 cctggacgat gcggttcgtc tcgcggacgg cgcgcacgac gtcgaggccc tgcaggggct
    15541 gggaggtcat gggcttctcg acgaagacgt ccttgccgga acggcaggcg tcgaccgtca
    15601 tgccggcgtg gaggtgatcg agggtggcga cgataacggc atcgatgtct ttgcggtcga
    15661 gcatccggcg gtagtcggcg tatttctcga cgggcttgcc gatgcgcttc gcggcttcgt
    15721 cacggcggac gtcccacgcg tcacagacgg cgacccactc gatgccgccg gcgaggttgg
    15781 ccatgttcat gaggtgcgag ccgcggccac ctgttccaat gatgccgagc cggatgcggt
    15841 cgttggcgcc gagcacacgg gacgccgacg ccgcggtgac ggcgccggca atgaaggacc
    15901 ttctggaaac gtcagaactc ataagatcga cactccctga ttgagttttt caacgaggcc
    15961 gcggagccag acgagactgc gctcgatcat cggcccgccc tgaccttcgc actccatgct
    16021 gagcacacca tcgtatccgg attcagcaag gattgtgagg caccggcgga tgttgtccgc
    16081 attgacgccc tcgccgatgg cacagtggct gacggcaatg ccggtcagat ctccccgggc
    16141 ggcagcggcg aggctctcgc tgacgtcctt gacgtgaacg tggctgacct ggtccttcag
    16201 gcgaacgaga aaggcgacgg ggtcctggcc ggcgatgaac gtattcccgg tgtccatgtt
    16261 catgcgcagg aacggcgagt cgcaaaaggc gagcatctcg gccatgaagt cgggcttcgt
    16321 agtgaagtat ccgtggggtt cgacgttgac ggtgatttgg taggcctcgg cgacgggaac
    16381 gaactgccgg tagattcggc gcatgtggtc gagggcctcg cgctcggtca tccctgcggg
    16441 cttacagcgg tcgtcggtgg tgtcgacgcg cgggcagccg gcgagtttgg cccagcggat
    16501 cgtgtggagc acgtattcga cgccgaggct ggcgccctca ggaagcgaaa gcggaaaagc
    16561 ggcgtcgatc tgagacatgc ggacgtcgta gcggtccatc ttccggcgaa gtagtacggg
    16621 atcctcccat agggctacgt ggggctggta gccgagtccg tggatccagc tcacgccatc
    16681 gatggcgcca cactcgatga agtgcaggcc gttgtcctgt gcccacttaa ggcactgctc
    16741 gaaactccaa tagctgctgt tgaaagcgtc cgtatgaaag ccgatttgga acatgcagaa
    16801 tctccgtcac aggcttaagc ccaatgctat cgtcgctaca gttaacataa accgttattc
    16861 ccctccgccg gtggttagca tgacaggaat gttaactgta tagccggaaa tattcccgtg
    16921 ataccccgta gacaataacc gttagcactc catctacccc cttcctcaaa ggtcgatggc
    16981 gacgatctcg tggacccgga tcgatgggac cacgaggctt agcgtaccat cggattcctc
    17041 gaatcgcggc gtccggccgc tctcgagcag ctgcactttc ttcgcccgca caccttgcgg
    17101 gagccggaca cggaccttct gctcggtgag ggggataatc tcccgaaagg gtcctttcat
    17161 catcatcgga ttggtcagat tcacaaggtg gacggtcatc gagtccttct gccgccagac
    17221 ggtgacatcg aggacgcccg ggccggtgac cgtcaccggc tgctcctcgt tcatggccca
    17281 ggctacggcg ttgcccagca agcggccgtg gtcgacacac atcacttccc agaagacgcg
    17341 gtcgatatcc caagggaaat agacgatgcg gctgggaccg atctcgcgga ggtagacctc
    17401 ggggatggac gtcttcggga cacgaggaaa gaccttctcc atgggcaggt ccgggtagga
    17461 ggggatcagc gtgagtgggg aattcgggaa ggactcccgg gcgccgacct cgacctggta
    17521 aaccccgtga atgatgcggg gtgcgtcctc gagaccggcg aggatcgggt ggcgcgtgtc
    17581 gtgttcgagg cggaggtgag aattctgcac cgggccctcg acgcgccctc ggtaccacac
    17641 gccgaacaga tcgctgagac cgaagtcggg ccgccgggtg ccccactcgt tgtagaggga
    17701 ggtttcgaag gtggcgagga ggctgccgcc gcgcctgatg tattcgcgga gctggtcgca
    17761 ctgggtgtcc gagagggcgg cgatgttcgg caacaggagg agcttgtagc gggcgaggcg
    17821 gtccggttcg agcaggcgat cgtggaccat ttcgaacggg acgcgggcct cgatcagcgc
    17881 gtggtagagg ccgcgggtgt ggtcctcgac cttctgcgtg gcgcgctcgc caccgtaaaa
    17941 ccaagcggtc tgctgcgagt agaccatgcc cacgcgagcg agcggctcct cattgcgaag
    18001 gtagcgctcg tgccggtatt gccagttgta gatgtcctcg acggtcttca gccagcgcga
    18061 atcgtggatc gttccggaga atttggtgaa ccaggggcgc aaaccgtttg cgaggccgtc
    18121 ggcgacccag atgcggattt cggcgccgtt ctgcacggaa tccgaccagc gatacggctg
    18181 cacgacgccg acactgaaga tgccgccgat ggccttgcgt ccgagagtgg cacggtactc
    18241 tttgccgttc ttaccggcgg cccacggtgc gttgagtccg atgcgggcct ggcggtcggc
    18301 gaagagcgtg ggggcgagtt cgccgatcgt cttcatgtcg agatcgctga gagcgccgcc
    18361 tccggcattc gcgacgaagc gggcgttcgg gttgattttg cggatctcgc cgtcccagag
    18421 ccgccagagg tcgaaaaggc gcttctgttg ccaaatgatg tagttgcggc gtgcagggtc
    18481 cagggggtcc cgtgtccggg gaagctccat gccgcagtaa tctttgaaat tgcgctggca
    18541 atgctcgcag tagcacatgc cggagccggc ccagcggttg gagaagatac cgtccacctt
    18601 gtagagggag acgatctcct tggtgacctc ggtcatgaac tcgaagttgt aggggccgag
    18661 ggtgcaggtg acccacatct cgggagaggc ccagtgccgg cgcttgcggc cttccgcgtc
    18721 gacggcgatc caatccggat gggcctcgta gacatcctgg taggtcgcat gagggtcagt
    18781 gcgggcgacg acgaccatgc cgagttggcg gcagccttca tacagctcgg caaaagggtc
    18841 ggtctcgccc atccagcggc tgcggtggtg gaagggaacc ttggtcgggt agtaggcgac
    18901 acacccgccg gcactgagac aggcagcgtc ggcgtggatc cggcggaagt aatcgagcca
    18961 gaatttcgga tcgtacttgc cggggtcgtc ctcgacgagg gtaagctgcg cccatcgcat
    19021 gggcttgtcg caccaagcag gctggtcctg gacttgcgcg gaaccggccg cgagcgcggc
    19081 ggacgcgccg atgaactgtc tgcgagtgaa atccatgagc acctcgtaac cggacatcgt
    19141 attacactgg cctcgatttt ggacgggagg gcgccctgcc gaatcgacgg ctccaacgaa
    19201 accggacgcc accgtcccag aatcggacac cccggttccg gtttttctct ttgtttcagt
    19261 cgattagcac tggcatgcaa gctgcttccg gaaaggctgt atggacgttc cacgcgaggg
    19321 tgtcgcccgg aagaagctga tccggcgcat tgccgccgga acggtggttg cagccgcgat
    19381 ccctttgatc acgatagggc tcgcccggct gaagccggcc gctcctacgg tcgaaaaggc
    19441 gacagtgtgg atggataccg tcaagcgtgg tccgatgacc agagaagtgc gcgggttagg
    19501 cacgctggtt ccggaagaaa tgctctggat accagccccg gcggacggac gggtggaacg
    19561 gattctggtc cgcccagggg cggcggtcga cgccgggacc gtgctgctgg tgctgagcaa
    19621 ccccgaactg gacctggctg cgctcgaggc cgagtaccag gtgaaggccg cggaggcgaa
    19681 gtacaaggac ctgaaggtcc agctcgagag ccagcggctg acgcaacagg cggaggtggc
    19741 ccgggttcag tccgagtacc accaggcgaa gctgaaggcg gaccgggacg aggcgctggc
    19801 aaaggagaaa ctcctccctg acctcgatct gaagatttcg gtggcaaccg cggcggagct
    19861 cgcgaaccgg cacagcatcg aagagaagcg cctggacatc cagtcggact ccgtggacgc
    19921 gcaactggcg gtgcagcggg ccgaggtgga caaggtgcgg gctctggcgg ggctgaagcg
    19981 cactcaagtg gaagccctgc gcgtgagggc cggagcggtt ggagtgcttc aggaattgcc
    20041 ggtcgaagtc ggtcaaaagc tggttgccgg tacgattctt gcaaaggtcg cccagccgga
    20101 gaggctcaag gcagagttga agattccgga gacccaggcg aaagacgtgc aactcggcca
    20161 gaaggtggcc gtggacacgc ggaacggcgt gattccgggc cgtgtgtcgc gtatcgaccc
    20221 ggcggtccgg gaaggcacgg tcacggtcga cgtgaagctg gagggttcgc tgccgcaggg
    20281 tgcgaggccg gacctgagcg tggatggaac gatcgaactg gagcacctgg cggacatcgt
    20341 gtatgtggga cgtccggcat tcggacagcc gaacagcgcg gtgacccttt tcaggacgga
    20401 cgcggacggg aagggtgcgt cgcgggtgca cgtaaagctg ggccgcgcgt cggtgaacac
    20461 aattgaggtg gtcgaagggc tgcggccggg cgaccaggtg attctttcag acatgtcggc
    20521 gtgggatgcg caagacaggg tgcggttgaa ctaagccgcc agcacgaaag cggagagata
    20581 aagatgaatt ccacggatac gcaattgatc aaactcgagg gagtaacgaa ggtctttctg
    20641 accgatgaag tcgaaacaca cgcgctggcg ggggtgcacc tggagattcg gaagggcgag
    20701 tacgtgtcga tcgccggccc gtccggctgt ggcaagtcga cgctgctttc gattctaggc
    20761 ctgctcgaca ctccgaccga cggacgatac tggctaaacg accggccggt ggaaagcctg
    20821 aaaccggccg agcgcgctcg catccggaac cgggagatcg gcttcatttt ccaggcattc
    20881 aacctgatcg gcgacctgac ggtgtacgag aacgtcgaac tgccgctgac ataccgcgga
    20941 atgagggcca gcgaccggaa gcggaatgtc caagaggcgc tggagaaggt cgggatgtcg
    21001 catcggacga aacactatcc cgcgcaactg tcgggtggcc agcagcagcg ggtggcggtg
    21061 gcgcgtgctc tggcgggcgg gccgtccatc ctcctggcgg acgagccgac ggggaacctc
    21121 gattcagcga acggacaggc ggtaatggac ctgctgagcg acctgcaccg gcaaggtgca
    21181 acgatctgca tggtgaccca cgacccgcgc tacgcactgt gcgcggaccg gtcggtacac
    21241 ctgtttgacg gccgggtcgt ggaggagagc gtcgaggcgg gaaggtagcg gctcgtcagg
    21301 cttctactgc cgataaagcc caatgagtcg cgagatccgg cacgccgtgc gggcattggc
    21361 taagagtcca ggtttcagcg cagccgttgt gcttacgctt tccctgggga ttggcgccaa
    21421 cacggcggtt ttcagcgtgg tgaacgcagt ccttcttcgc ccgcttccct ttgaggaccc
    21481 cgagaggctc gcgctcatct acgagtcccg cccaaggatc ggttccgaga ggtcgtcgtc
    21541 atcggaggcg aattactttg gctggcgtga gcggtgccgc tcgctcgaac ggatcagcgc
    21601 cttcaacctc catttcttca cgctgaccgg agtggagagt cccgaagaga ttcagggcgc
    21661 gatcgtgtcg gcggacttct tcccgctgct cggcgtgaag ccgtacgcgg gaagaacgtt
    21721 ctcggcggac gaggaccggc ccggtaacaa ccaggttgtg ctcctgagtt acgagctctg
    21781 gcagaggcag ttcgggtcag acccgggagt catcgggcgg aagcttattc tcaacgagaa
    21841 gagcttctca gtgattggga tcatgccgcc ccacttctcg tttccgcctg tcgggcaggc
    21901 ggggttgtgg gcgcccatct ccctcgatgc ttcgccgccg gagcagtttg ccagacgctg
    21961 gtacctccgc gtgctcgcgc gactgaaacc tggggtaacg tttgagaagg cgcaggtgga
    22021 gttgagcgcg gtctcggccg ggctcgagcg tcagtatccg gaggcgaacg cgggcttccg
    22081 cgcgcacctg gcgccccttc acggagaact ggtgcggagc gtgcgcacat ccgtgctgtt
    22141 gttgtggggc gcggtgggct tcgtcttact gatcgcctgc gcgaatgtcg caaacctgct
    22201 actggcgcgg gcggctcgac gcaagaggga gattgccatt cgcattgccc taggcgcagg
    22261 tccctggaac agggtcagtc aaaccctctc cgaaaccgtg gtgctggcgc tatcaggcgg
    22321 cgtgatcggt gtgtttttcg cctggtgggg cgtggacctg ctcagccggt tgggcctgga
    22381 agccgttcca agctcgggcg aaatcggtgt ggatgttaag gttctgacct tcacactcgg
    22441 tatctcgctg gtcacgggaa tcattcttgc tttgtttccc gtctggaact caaggatacc
    22501 ggacattccg ggtgctttga gagagggcgg atcgaggagc cgagacgaga ggaacccgag
    22561 ccgggcgcgg tggattctga tcgtttccga actggctctt tccctggtat tgttgaccgg
    22621 ggccggactg atgttagaga gccttcggcg gttgtccgcg acagacccgg gattcgaaac
    22681 cggcaacatc ctcacgatga gcctgtccca tccgcgctcc agattcgggc cgctcaaagg
    22741 gagagcggac ttcttcgcac agcttcttga gcgcatcgaa catctgccgg gagtccgggc
    22801 cgtcggcgca gtgaatgctc ttcctttgag cgggagcgac tcgtcgagca gtttcgtcat
    22861 tgaaggcaga cccgcgcagc cgcacggccg attcacctcg gcgcagatcc gtatcatcac
    22921 gcccggctac cttcgcgcga tgggcattcc attggtcgcc ggacgcggct tcactcacgc
    22981 agacgcggcg aacacaccca gggtcgccat catcaaccgg gccatgtccc gctggcagtt
    23041 tcgaggtgag aatccgttgg gccggcggct ggcctttgac accagcggtc cgttccggga
    23101 gatcgtaggc gtcgtcggcg atgtgcggca cgagcggctc ggcgtggaac ccgtgccgga
    23161 gatctacctg ccgcacgctc agtcgccact cgcctcgatg atgcttgcgg taaggacgac
    23221 cgacgaacca agcatggtaa ctcccgcgat ccgcgccgag gtccacgcgc tcgataagga
    23281 ccagccggtg acgggcgtgg cgacgctgga cgaacgtctg gaaagatcca tgtcgcgttc
    23341 ccgtttcgat gcggtggtgc ttggactctt cgccgcgctg tcgtgcgcgc tggccgtcgt
    23401 cggcgtctat ggtctggtga actactcggt ggcacagcgg acccacgaat tcggggtacg
    23461 tctagcctta ggcgcggata agggagaggt cgtgtggacc gccctgcgag agggccttgg
    23521 actgacggcc gccggaatcg ggatcgggct ggttggagcc attgcgttga cgagcgtact
    23581 gtcctcgttg ctctactccg tgaggccgcg ggacccggcg gtgctcttag cagcaggcct
    23641 gctgctcgcg gccacatcga tgctggcgac gtggctccct gcccgtcgtg cggcgaaggt
    23701 cgatccgatg aaagcgctgc gctatgaata ggaggtggcg gtgggaatcg tgagtgactt
    23761 ccgcattgca cttcggatgt tggcacggag cccgggcttt gtcgtcgtat cggtgctgtc
    23821 gctggcattg ggaattgggg tcaacgtctc ggttttcagt tctgtcaacg cgctgtttct
    23881 ccggccgttg ccggcggtgc cggagtccgg gcggcttgtg tcgatctacc accgcactaa
    23941 gtcgggcagc ttcacatcca gctcttatcc tgactacacg tactaccggg aacagaacca
    24001 tgtgttctct gggatggcag cgtatctcag attccccacg aaccttcgat cgggcgaaca
    24061 gactgagcgg atttatggcg aactcgtcag ctccgactac ttctccgtcc tgggtctcag
    24121 tcccgctctc ggccgactga tcgtacctga cgaacgtgag cccgtcgccg tgctgagcca
    24181 tgacttctgg cagcgccgct ttgggggaaa ccgtggcgta atcggtcagg cagtgagact
    24241 tggaagcggg ctgttcaccg tggtgggggt ggcgcctcga gaatttcgcg gcatcgttct
    24301 ggattgggcg gaatcaccgt ctgtctggtt gcccgtgtac acatacaagg aagcgttgcc
    24361 aacaccacca tttggaacag tcgatttcct gcattattgg ggcaaccaaa gttacgagct
    24421 cgttgggagg ctgcgtgacg gagtgactct gggccaagcg gccgccgaga tgacagcact
    24481 cgccaagcgc attcaaaagg atcatccgga acgcgcccga gtctggtcga aggaaatggc
    24541 cgaatacggt gacctcgacg ccgttgtctt ccccacgaac cggatgcggc tctggccggg
    24601 ggaccggagc aatctgatcg atttccttgt gttgctggcg gccgtcgccg gcctggtcct
    24661 gctgattgcc tgcctgaacg tggccagcct cgtccttgcg cggactacga agcgtcggcg
    24721 cgaactggcc atccggctct cgcttggagc gggccggtgg acgattgtac gacagttact
    24781 cgccgagaac ctgttgctct ccttgtttgg aggcgctatt gggattctgg tgtcaggctg
    24841 gacatcgacg ttcctggcca gctttcatca agcttttcgt ttgccggtgc cgccggagac
    24901 agccgtcgac gttcgggtgc tggccgccgc caccggagtc tcagtggtga gtgccgcgct
    24961 gttggtactg tttcccgcac gactggcttt tcgacttgac gtgaacacag ggctcaagac
    25021 cgacatgggc accaccagtg cgtcagggtt gcgcgcgcat cacgcgctgg tcttgggaca
    25081 agtggcgctc tcggtcgtga tggtggtcgc ggcgggtctt ttcgtgcgaa cactgtggaa
    25141 cgcgcaggct gaggacttta cgagccaatc agaaaacgtg cttttgggga gcctggacct
    25201 gggctcgcgc ggctacgatg gcgaccgtgg gggccgcgtc tattcgcagg tgttggagcg
    25261 ggtacgcact gtgccgggag tgcgcgacgc ggcgctggtg tttgtggtgc cgctgggcgg
    25321 acggcgcggt gggaccaacg tcgtgattac tccgccggac ggcggggtga agcggagcgt
    25381 tcagatcggt ctcaacgtcg taacacccgg ctactttcag atgatcggta tccccgtcgt
    25441 tcgtggccgg gatttttcgg atgcggaccg ctccggcgcg ccggctgtag ccatgatcaa
    25501 tgacgttatg gcgcaaacgc tgttccccgg acgtgaaccg gtgggacaac ggttcctgtt
    25561 gacgtggcct ccggcggcga cagtcgaagt gatcggcgtg gtacgggacg ggaagttccg
    25621 caattaccgt tccgagagag agccgaccgt ctacgttccg ctggcgcagc gattcatgat
    25681 ggcgatgaat ctggaagtcc gcacagcagg gaacccgtcc gcgctggcgg ccgcgatcaa
    25741 ccgcgaggtc gcggcagtgg acaaggactt acctctcaac gccttccaga cactgaagac
    25801 gcacttcgag accgcactgg cacgggagcg gctcactgcg gccctgctta gcgcccttgg
    25861 tgctctggcc ctggtactga cagccatcgg tctgtacggc gttctttcgt acgcggtatc
    25921 ccagcgtaca cgcgaaatcg gcatccggat ggcgctcggc gctcgatcgc aatccgttgt
    25981 gcgagcagtt ctcggcagca cgatgcggat tgtgaccgcg ggcctgggca tcggggtggc
    26041 cgtcgcgctc gccctctcca gtgtggttag aggtttgctt tacggcatca cgcccacgga
    26101 ccccgccgtg ttcgccacga cgattctcgt gcttgccggc accgcggcgc ttgccagtta
    26161 cttgcccgcc cggcgcgcag ctaaggtcga tccaatgaag gcgttgcggt acgagtgaaa
    26221 gcgaggaagc gatgattcaa ctgaataata tcgagaagca ttacgacgtc ggcccgaaca
    26281 agttcttcgt gctaagaaga atcaccatgg aggtgaagga gggcgagttc gtcaccatca
    26341 tggggccgtc gggggcgggc aagtccacgc tgctgagcat cgtcgggatg ctggacggcg
    26401 cgtggtccgg cgagtatcat ttttttgacc agccggtgca cgcgctcaag ccgaaacagc
    26461 ggatcgagct gaataagaag tatatcggct tcgtgttcca gagctatcat ctactggacg
    26521 acctgacagt gtacgagaac ctcgacctgc cactctcgta tcgcaacctg aagggttccg
    26581 agcgtgcagg gatcgtgtgc gacacgctcg accgcttcca gattgtaggg aagaaagacc
    26641 tctacccgag ccaactttcc ggcggccagc aacaactcgt ggcggtggcg cgggcggtgg
    26701 tggccaagcc gaagctgatt cttgcggatg agccgacagg caacctccat tcggaacagg
    26761 cccaggagat catggagctg ttcaagaaac tgaacgacgt cggcacaaca atcatccagg
    26821 tgacgcactc ggaacggaat gcggcttacg ggaaccggac catctatttg gaggacggct
    26881 ggatcgcaaa ggaccggccc tcgggctatt gagccggccg cctaacgcgt ccgatcctct
    26941 ggcctacact gaaggactag cttgatgaaa ccctccgacg cagtcacgcc gcgcattctg
    27001 gtagcagacg atcagcccga cgtactcgag gcgctccggc tgctgctgaa accggaggga
    27061 taccggatcg agacagcatc ttcgccggcg agcatcatcg cggcactgga gtcccaggac
    27121 ttcgacgtgg tgctgatgga cctgaactac gcccgcgaca cgacctcggg gcaggaaggt
    27181 ctcgacttgc tgcaccggat tcaggcgatg gacaagacgc tccccgttgt gctgatgacg
    27241 gcttggggca gcatcgaact ggccgtggaa ggcatgcgcc ggggtgcgcg cgattttatc
    27301 cagaagccgt gggacaacgc ccgcctgctc gccattctgc ggacgcagat cgagctcgca
    27361 cgggcattgc ggcaggggga gcgattggag gcggagaacc gcgtcctgcg cggggatggc
    27421 gctccggtga tgatcgccga ttcgccggcc atgcagccgg tgcttcaggt aatcgcgcga
    27481 gtcggcccgt ccgacgccaa cgtgctgatt accggcgaaa gcggcgtggg aaagggtttg
    27541 atcgcccggg cgctgcatgc agcctcgctg cgcgcggtgc gcccgttcgt gaccgtcaac
    27601 accggcggcc tctcggaaag tctgttcgag agtgagctgt tcggccacgt aaagggggct
    27661 ttcacggacg ccaaggcgga ccgtgtaggc cgtttcgaac tagccgaaaa cggcactctt
    27721 ttcctggacg agatcgccaa cgttccgctc agccagcaag cgaagctgtt gcgggcgatc
    27781 gagacgggcg agttcgagcg cgtgggctcg tctcggacgc gccgggttga cctccggctg
    27841 atctcggcaa ccaacgctga cttgcgcgcc gaagtcgcgg cgggccgttt ccgggaggac
    27901 ctgctgttcc ggttgaacac agtggagatc ccgattcccc cgttgcggca gcggcgggag
    27961 gacgtaggtc cgctggctac gtatttcctc cggcaacatt gccagcgcta ccggaagtgc
    28021 ctcggcgcat tcgagccggc cgcgatgcag ttgcttctga gccatacctg gccggggaac
    28081 gtccgcgagc tcgaccacgc ggtggagcga gccgtgctga tggcgcaggg agaagcaatc
    28141 cgcgccacgg acctggcgct gcggcaacca cgcgaaggct ccggccggat tgaggacatg
    28201 agcctcgaag acgtggaggc cttcctgatc aagaaggcgc tggcgcgatt cggcggcaac
    28261 gtcacacaag ccgcgcatac cctgggtctt agccggagcg cactctaccg gcggcttcag
    28321 cgttacggtc tatgagcgcc ggcggaagaa gaaccagatc cagattcgcc tacgagaccc
    28381 gcctcaccct gcttgcactg ctgaccggca cgcccgccct ggtggccgcg atgattctgc
    28441 tctggaccgg tggtttcaca ccgaaagttc agtggacggt gacgattgtc gtcgtctgtt
    28501 tctggttggg agcggctttc gcgttgcgcg agcgggtcgt gcgtccgctg cagaccctat
    28561 cgaacatgct ggcggcgttg cgcgaggaag attactcgat gcgcgcccgg agcggtcgtt
    28621 cggacgacgc gctcggcgag gtgtttgtgg aggccaacgc actcggccag atgctgcgcg
    28681 aacagaggct cggagcgatg gaggcgacgg ccctcttgcg cacagtgatg gtggagatcg
    28741 acgtggcggt tttcacgttc gacggcgagc agaagctacg cctggtgaac cgggcgggag
    28801 agcgcctgct cggacggccg gcggatcggc tgctcgggcg caccgcggag gaactggcgc
    28861 tggcgggttg tctcgatgga gaaccggtgc gcgcgatcga actagcgctg ccgggcgcct
    28921 ccggtcggtg gggcgtccgc gtcagcacgt tccgggaaaa gggactcccc catcgcctgc
    28981 tcgtgctggc cgacctaacg agaaccttgc gggaagaaga actgcaagcc tggaagcggc
    29041 tggtgcgggt gctcgggcat gagctgaaca actcgctgac gccgattaag tcgatcgccg
    29101 gcagcttgcg gacgctgctg gagcaggatc agcggccgcc cgattggaaa gaggacacgc
    29161 agcgtggtct cgacgtgatc gccgcgcgcg cggaggccct cagccggttc atggaggcct
    29221 atgcgcgact ggcaaaactc cccccgccga agctcggctc cgtggacgtg gaggcgtggg
    29281 tgcgtcgggt ggcaggactg gagacgcgcc ttccggtcac agtggaagag ggaccggcga
    29341 tcctcattcg gggcgacggc gaccagctcg atcagttgct gatcaacctg atccgtaatg
    29401 cagccgatgc atcgcttgaa actcacggag gtgtgcgaac gggctggcgg aggataggcg
    29461 gccacctgga gatctgggtt caggatgacg gcccggggat ctcgaacacc tccaacctgt
    29521 ttgtaccgtt tttcacgacg aaggcgagcg gttcgggcat cggcctggtg cttagccggc
    29581 agatcgcgga ggcacacggc ggcgtactcg ccctggaaaa ccggtcgccc ggaccggggt
    29641 gcgaggcacg gctgcggctg ccgatgtgac ggcggagcac cattccattc acaatgttct
    29701 gtatgcgact tgtgcatctg atgctctgct tcatcgtgtt gctggcggta tcctgccgca
    29761 ggacgccatc gaatgaacgc ccgaaagatt cgcactccta ctccaatccg gagctggtcc
    29821 gggtgcggca cgtcgatctg gatctggagg cgttcttcga tcagaagact ttgcggggaa
    29881 cagcggtgct cgaactggag cgagcaaagg acggcgagag cgcgcctttg atcctggata
    29941 cgcgcgacct ccggattgag ggtgtcgagg gcagcctgga tggcacagac tggagtccgg
    30001 taaagttcga agccggcgcg atggatccta tcctgggtgc gcccctgaca atccacctgc
    30061 ccgccggcgg gaagcacgtc agaatcggtt acatgacgag cccgaatgcc acggctcttc
    30121 agtggctgga accggcacag acagcgggaa agaaactgcc gttcctctat acgcaatcgg
    30181 aggcgatcca tgctcgtagc tggattccac tccaggacac ccccggcgtt cgcgttacgt
    30241 attcagcccg catccgggcg gccaagggct tgcgggcggt gatgagcgcc gaacacgttg
    30301 agacaggagc tgagggcgag ttccgctttc gaatggcgca gccgatcccg tcgtatttga
    30361 tcgcactggc cgtgggcgac ctggccttcc gcccgctcgg ctcgcggaca ggcgtctacg
    30421 ccgaaccgcc cgtggtggat gccgcagcga gggagtttga agacaccgag aagatgatgc
    30481 aggcggcgga ggaactctat ggaccgtacc ggtgggggcg atacgacctg ctgattcttc
    30541 ctccgagctt tccctacggc ggaatggaaa atccccgcct gactttcacc tcgccgaccg
    30601 tcatcgcggg cgacaagagc ctcgtttcgc tcgtagcgca cgaactggct cattcttggt
    30661 ccggcaacct ggtaacgaac gcgacctggg accacttctg gctgaacgaa ggattcaccg
    30721 tttacgtcga acagcgaatt atcgagaaga tatacggaaa agaacgggcg gaaatggaag
    30781 ccgtccttgc gacggagtcg ctgaagcgcg agatggagcg gcttgagccg cgcgaccagg
    30841 cgctgcaact tgacctgcgc ggcagggacc ccgacgccgg ctccaccgat gtcgcctacg
    30901 tcaaaggagc gcttttcctg aggacgatcg aacgtgcggc ggggcgtccg gccttcgacg
    30961 cgttcttgcg ttcctatttc gatcgattcg ctttccgaag cgtcacgacg aaggacttcc
    31021 tcgattgtct ggcgaagaat ctgaagacga atgtcccggt ggaggagtgg gttcacaagc
    31081 ccggactccc atccggagcg gccctcccgg tttcggatgc gctggccaag gtgagtgatc
    31141 tcgccggcca gtgggcgcag ggcatgattc cgtcccgcac cattccgtac gcgaaatgga
    31201 gtacgcagga acggatccaa tttctccaag cattgccggc ggatccaggc ggtcgaagga
    31261 tgcaagcgct cgatcaagcc tttggcatca caagaagcgg aaacgccgag gtcctccagc
    31321 cgtggctgct gatgtcgatc cggaacgggt acacgccggc ctcggcaaaa ctcgaagagt
    31381 ttctgatgag ggtgggccgg atgcggtaca tccgtcctct gtacgaggag cttgcgaaga
    31441 cgcccgaggg catggagcgc gcccgtgcga tctttgcgaa ggcgcagggc ggctaccatc
    31501 cgatcacggc aacagctatc gaagcggcgc tgcagcgtcc caatcgctaa cccggacgtt
    31561 ggcccccggt cgaggggcgt gctacgttgt cagttatcag aggaagggag tccggataga
    31621 gattcccgct catctactca agccgtggag gaagccaaca tgaggagaat tttgggtaac
    31681 gttcttgcac ttagtgtgag tgccgtcgcg attcttgccg gcgcgccgcg cactgcgact
    31741 gagaaaccgg gaccaccgcc gaagtcgggc ttctattcgt tctggaacat tggagaggag
    31801 tttggcgagg ggattggaaa cgtttcgaag ctgttctgcg agatcggtac ggcaacccag
    31861 ccgccgatct acaccggtaa catgctcatc gattgtgacg gcgaggtgcc gcacaacgaa
    31921 acgagcatcg ccgtcaaccc gaatgatccc aagcacgtgg ttggcgccta ccactcgtac
    31981 caggttaagt tccgcggcgc gacagcg
//
LOCUS       DATIFQ010000231         9867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1415361, whole genome shotgun sequence.
ACCESSION   DATIFQ010000231 DATIFQ010000000
VERSION     DATIFQ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415361"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1725)
                     /locus_tag="VLE22_10745"
     CDS             complement(73..1725)
                     /locus_tag="VLE22_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HSB14927.1"
                     /translation="MALPHRNAVVIGAGAGGGIVAKELAAAGLSVVLIERGKWYSAFD
                     CRKDDLRNQRTSILGSSSGPDEQRNPRVLVDANGREQIVYPSQGGYNNNAACVGGGTL
                     TYGAMAWRFMPQDFRMRSTYGPLEGSTLEDWPITYDDLEPYYEKAEWEIGVSGGDANN
                     PFKGPRKKRLPMPPLDPTHEMKILRPAAEKLGLHPFDIPMLRNSVPYNGRQACMRCRW
                     CVGFACEVNAKNGTQNTVIPTAMATGNCELRTECMVKEIQTDSKGRATGVAYFDADDR
                     LREQPADLVVVSCCAIETARLLINSKTKLFPNGLGNRHDWVGRNLQGHTYTGAYGLFE
                     NVAYDDLGPGAGIAVSDYNHGNPGLKGGGMLCNEFIRLPIQAIGIAPPGTPRWGKGHK
                     DYMRKWYRRCVSFRGPTQEMPVFDSRVQADPKVKDYWGIPVARMSGDKHPHSIEIGNY
                     MAEKAERVLKEAGAILTWKRGGGKGLSGGQHQAGTCRMGNDPKTSVVDQYCRVHEIDN
                     LFVVDGSVHVTNGGFNPVLNIMAIAYRASEHMVKTWKGTKFR"
     gene            complement(1742..2305)
                     /locus_tag="VLE22_10750"
     CDS             complement(1742..2305)
                     /locus_tag="VLE22_10750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HSB14928.1"
                     /translation="MKGSKTLKRRKFIQVAAAAAASGPLVSCGGPKPPWRFFTAGEAA
                     VVNSLCETIIPADQDPGAGWAGVVRYIDRQLHRWFKENRETYRLGVAAIEKTAQKAHG
                     KSFVELSPDERTAMVASIEKGQAPAELWQGVSQKEFFDLILNHTMQGFYGDPRHGGNR
                     DMVSWKMLGVPATAIRGRQHYEFPPQS"
     gene            complement(2371..5022)
                     /locus_tag="VLE22_10755"
     CDS             complement(2371..5022)
                     /locus_tag="VLE22_10755"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HSB14929.1"
                     /translation="MMTVSRRHFLESAAVVSAAGVVGMAESADVAGDGPASTGFMPVA
                     LAGNTNIDALAQAGLSEEMAKAASKAPRGATVSWGIPFRIDRPVVLKDGPVTEKTPGL
                     KAEWLVFLHTADSRPVEKDQRGLIRPMRGEGFLAEHAADYMIVYTDGTEVREKIRRRH
                     QLGAFQPMWGENCFLAVAHNKPRAIRPVHEQPSMLVDAGGELGWGSAETRADSADRDT
                     WVNWLWAWKNPHPEKEIAALRFEPKSGLTVLSAVSAGSATSEPLRWGTRKKAILTLPE
                     GARFDYTIDSRGLSKQIQLDMGQVISAVPRSLYPNEDWSATYNNRVPEISARDVIIEY
                     TAHPDARFHLWDGKTVPVSSLEARSASTPLIAVTPATRRVEIRVVEQTSAKPVPVKLH
                     IHGESGEYLPPADRHRQPNGGWFEDYAPEFQHQDRHLCTYIPGETIVDLPLGNVYLEV
                     SKGFEIKPVRKTVRVTPDTQKIEVALEKVLPWREHGWVTADTHVHFLSPQTAWLEGSS
                     EGVNVVNLLASQWGELMTNVGDFDGKTTFGSKEAGGNGEWLVRVGTENRQHVLGHISL
                     LGYNGSVIAPMCAGGPDEAALGDPVGVLLMEWAERCRAQGGVVVLPHFPNPRCENAAG
                     LTTGLIDAVEMTSWGSFYAGIDPYSLSDYYRYLNCGHFIAAVGGTDKMAASTAVGTIR
                     TYARIPSGREFTYESWKDAVRAGNTFATYGPLVEFAVDGRPSGGRIEMKSGGGTVEVT
                     WEVASVTVPMSKVELVVNGEVRESRSVKPDRDSGRWPVKIDRSSWVALMVRGHYPDKP
                     EIIAAHTSPVMIQVEGSRFFSAVDAVTMIDQIEGALAFIDTIGTRAEDEVYKRVRLKL
                     MSAYRALHNELHQAGHFHEHTAVTAHK"
     gene            5167..6567
                     /locus_tag="VLE22_10760"
     CDS             5167..6567
                     /locus_tag="VLE22_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14930.1"
                     /translation="MPDNSFSLTRRQFGLSLGASTVAAANAVPPTLPEPWMGPAIVKK
                     VYLAKPNPTWPRPDLNIQQEIAAIEPHLSELERKHPGRIRFTGGDLLKVGDDISGWAR
                     SLEGADAILVIDLTSGLGPLLQKMRDIEVPMLLFTRPYAGWAYMDFTAWRQSGKRVEL
                     VASSEFGDLDPYMRIFQTIHHLKRSKVLMVAPGDGWRQTAAAYTRHYGTTIEFVSYQD
                     LKAAFDAADVSKAKQEAAEFTRRASRVVEPSPQEVLDAMRFYLGVKNILERAKANAIT
                     VDCLGGFRRGDLPAYPCVAWSKLNDEGFYGVCEADLASTMTQLLLTSFSGKPCFVSDP
                     VFDTSRSEIIHAHCVSATAMQGLGGPGLPYSVRSHMEDNKGVSMQVLMPVGDTVTVAK
                     YDGPTKLLVSTGEVLGNVDNPRGCRTKFRTRVPGARKMLENYTAGLHRVVVWGDYTEP
                     IESMRRLMGFDIVREA"
     gene            6572..9181
                     /locus_tag="VLE22_10765"
     CDS             6572..9181
                     /locus_tag="VLE22_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14931.1"
                     /translation="MSLSRRHFLLAGPLLPALRGAAPAGLRESEPVPEPHFPSRLYQF
                     VWRNWELANTDRMAEVAGATPRQILDLGESMGLPAKPSLSEDQLRRIYITVIRQNWHL
                     LPEPQIIKLLGWSDERFRFTLKEDDFLDIKLGLNKPRCPELRYDPPSEADQHAAARIR
                     ETLRAVFGDSLRAPGQRSFQFVEELSDLRFVPMRDPSARPAPTEIDLTSGWSIAEPEG
                     DVLASAARRLREFLRDAMGVSTATGGKHIVLEVRPPGNGPGSFDIAVSEAAVRITGDS
                     DSGALQGVYWLMDQMEEREAPFLRPGVTSRKAVWNPRFLYSHFALYGDPLMEPEADPF
                     PDAYLEKLARRGINGVWMQAVLNNLAPSKQFPEFGEGSETRLRNLDALVRRAARFGIS
                     IYLYLNEPRAMPPEFFRAHPETRGSSFLGTNAMCTSVPAVREWIADALTHVVRHAPGI
                     GGFFSITMSENHTNCFSHGGAWGKAAPNAGDCPRCAQRKSWDVIAELITTFRDGVRRG
                     GSSAHVIAWDWGWGDDLTANLIPLLPKDVRFQSISEWDQPVNRGGVPMRVGEYSISVV
                     GPGPRATRNWERARKSGVTAMAKTQFNNTWEISAAPWIPAVHLIARHCENLTKAGIAG
                     IMASWTCGGYPSPNLSAAKAFYFEPRESTPAVLRSVAARRYGRAAAGEAVEAWRRFSE
                     AFEEYPYGVAVYIIPTQHGPANLLRLEPTGHAPGMILFPHDDYKAWCGKYPPEVVQRQ
                     FGKMAALWNEGLTAFRDALAKVSPRKKANASADLAVAETCYHHFRSTANQVEFYILRD
                     RLRSVSDAERAQITARMRAIVEDEIDLARRQYWVARANSAIAYEASNHYYYRPLDLVE
                     KVLNCRDILARLK"
     gene            complement(9185..9844)
                     /locus_tag="VLE22_10770"
     CDS             complement(9185..9844)
                     /locus_tag="VLE22_10770"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HSB14932.1"
                     /translation="MRKYGVVAVLSLLAGAFAAAQGPVITGEYIEDRANRVFGCYCEW
                     SGERVTGGKEAILGWRFHSGEYAGASLSGVTAAAVVMGEASLSAGDAPRKSILFIDNH
                     ASGDQRQAVERLLREKHGSLLGNILSVQATPIEFRRDVEHATLRIGDLVNVELRKAKP
                     LQDALQGAVLWYDPFIPMKESTLGMTVNTRYQGHEFDKQWNMNEPGISGYFGTFAVEA
                     R"
BASE COUNT         1691 a         3420 c         2893 g         1863 t
ORIGIN      
        1 gccaggagcg ccgccaccac ggccacgccg agaaacaatg tcgatcgctt cggtctcttc
       61 atggcgtggc tcctaccgga acttcgtccc tttccatgtc ttcaccatgt gctccgacgc
      121 ccggtaggcg attgccatga tgttcagcac cggattgaat ccgccgttgg tgacgtggac
      181 gctgccgtcc accacgaaca ggttgtcgat ctcgtgaacc cggcagtact ggtcgaccac
      241 cgaggtcttc ggatcgttcc ccatccggca tgttcccgcc tgatgctgtc ctcccgagag
      301 gcccttgccg ccgccgcgct tccaggtcag gatcgcgccc gcctccttca gcacccgttc
      361 cgccttctcc gccatgtagt tgccaatctc gatcgagtgc gggtgcttgt cgccggacat
      421 ccgggctact ggaatccccc agtagtcctt caccttcgga tccgcctgca cgcgcgagtc
      481 gaacaccggc atctcctgcg ttggcccgcg gaatgagaca caccgccggt accacttccg
      541 catgtagtct ttgtgaccct tcccccaacg cggcgtgccc ggcggggcga tgccgatcgc
      601 ctggatcggc agccggatga actcgttgca gagcatcccg ccgcccttca accccgggtt
      661 gccgtggttg taatcggaca ccgcaatgcc cgcgcccggc ccaaggtcat cgtaggcgac
      721 gttctcgaac agcccatagg cgccggtgta ggtgtgcccc tgaagattac ggccaaccca
      781 gtcgtgccgg tttcctaggc cgttcgggaa cagcttcgtc ttcgagttga tcagcagccg
      841 cgccgtttcg atcgcgcagc aggaaaccac gacaaggtcc gccggttgtt cccgcaggcg
      901 gtcgtctgcg tcgaagtagg cgaccccggt cgcgcgcccc ttcgaatcgg tctgaatctc
      961 cttcaccatg cactcggtgc gaagctcgca gttcccggtg gccatcgcgg tcgggatgac
     1021 cgtgttctgc gtgccgttct tcgcgttcac ctcgcaggcg aacccgacgc accagcggca
     1081 ccgcatgcag gcttgccgcc cgttgtacgg cacgctgttg cggagcatcg ggatatcgaa
     1141 gggatgcaac ccgagtttct cggcggccgg ccgcaggatc ttcatctcgt gcgtcggatc
     1201 cagcggcggc atcggcagcc gcttctttcg cggccccttg aacgggttgt tcgcatcgcc
     1261 cccggagacg ccgatctccc actcggcttt ctcgtagtag ggctccaggt cgtcgtaggt
     1321 gatcggccag tcctcgagcg tgctcccctc gagcgggccg tacgtcgatc gcattcggaa
     1381 atcctgcggc atgaagcgcc atgccatggc gccgtaagtg agcgtgccgc cgccgacgca
     1441 ggccgcgttg ttgttgtagc ctccctggct gggatatacg atctgctcgc ggccgttcgc
     1501 atccaccaga acccgtggat tccgctgctc atccggcccg gaactgcttc cgaggatcga
     1561 cgttcgctgg ttccgcagat cgtctttgcg gcaatcgaac gccgaatacc acttgccgcg
     1621 ctcgatcagg acaacgctta gtcccgcggc agccaattcc ttcgcgacga tgcccccgcc
     1681 ggcccctgcg ccgatcacga cagcattccg gtgcggtagt gccatggttt ctcgtctcct
     1741 ctcaggactg cggcgggaac tcgtagtgct gccggccgcg aatggctgtc gccggcaccc
     1801 cgagcatctt ccaactcacc atgtcccggt tgccgccgtg ccgcgggtcg ccgtagaagc
     1861 cctgcatggt gtggttgagg atcaggtcga agaactcttt ctgggatacg ccctgccaga
     1921 gctcggcggg cgcctggcct ttctcgatcg aagcgaccat cgccgtccgc tcgtccgggc
     1981 tcagttccac gaagctcttc ccgtgggctt tttgcgcagt cttctcgatc gcggcaaccc
     2041 ccaaccggta agtctcgcgg ttctctttga accagcgatg caactgccgg tcgatatagc
     2101 ggacaacgcc ggcccacccg gcgccggggt cctggtcggc cgggatgatc gtctcgcaga
     2161 gagagtttac gaccgcggcc tcaccggcgg tgaagaaccg ccagggtggt ttcggcccgc
     2221 cgcaggaaac cagtggaccg gaagccgctg ccgcagcggc cacctggata aacttccggc
     2281 gtttgagagt cttggacccc ttcatcgcaa tcctcgcccc tattcatatc acaccggggg
     2341 cgggctcttc agaaggacca agcgccagcg ctacttgtgc gccgtgaccg cggtgtgctc
     2401 gtggaagtgg ccggcctgat gcagctcgtt gtgaagcgcc cggtaggccg acatgagctt
     2461 cagccgtacg cgcttgtaga cctcatcctc ggcccgcgtg ccgatcgtgt cgatgaacgc
     2521 cagggcgcct tcgatctggt cgatcatggt caccgcgtcc acggcagaga agaaccgtga
     2581 cccctcgacc tgaatcatca cgggcgaggt atgtgcggcg atgatctccg gcttgtcggg
     2641 gtaatggccg cgcaccatca gcgccaccca ggaactacgg tcgatcttca ccggccaccg
     2701 gccggaatcg cggtcgggct taacgctccg gctttcgcgc acctccccgt tgactacgag
     2761 ctccaccttg ctcatcggga ccgtgacgct ggcgacctcc caggtcactt ccaccgtgcc
     2821 gccgcccgac ttcatctcaa tcctgccgcc cgacggccgc ccgtccacgg caaattccac
     2881 cagcggcccg taggtcgcaa aggtgttgcc tgcccgaacg gcatccttcc aggactcgta
     2941 agtgaactcg cgaccgcttg gaatccgggc gtatgtccgg atggtgccta ccgccgtcga
     3001 tgcggccatc ttgtccgttc cgccaaccgc cgcaatgaag tggccgcaat tcaggtagcg
     3061 gtaatagtcc gacagggaat acggatcgat ccccgcatag aagctccccc aggaggtcat
     3121 ctcaacggca tcgatcaatc cggtggtcaa gccggcggcg ttctcgcatc gggggttggg
     3181 gaagtgcggt aacaccacta cccctccctg cgcccggcag cgctcggccc attccatgag
     3241 caggaccccg accggatcgc ccagcgccgc ctcgtccggc cctcccgcgc acatcggcgc
     3301 gatcacgctg ccgttgtacc caagcagcga gatgtggccg agcacatgct gccggttctc
     3361 ggtgccgacg cggaccagcc attccccgtt tccgcccgcc tccttcgaac cgaacgtcgt
     3421 ctttccgtcg aagtcgccga cgttggtcat cagctcgccc cactggctgg cgaggaggtt
     3481 gaccacgttg acaccttcgg aagagccttc cagccaggcg gtctgcggcg agaggaaatg
     3541 cacgtgggta tcggctgtca cccagccatg ctcgcgccat ggcaggactt tctcgagcgc
     3601 cacctcgatc ttctgggtat cgggcgttac ccggaccgtc ttccgcactg gcttgatctc
     3661 gaagcccttg ctgacctcca ggtagacgtt gccgagcggc aggtcaacga tcgtctctcc
     3721 ggggatgtac gtgcagaggt gccggtcttg gtgctggaac tcgggtgcgt agtcctcgaa
     3781 ccaccctcca ttcggttggc ggtgccggtc cgccggcggc aggtactcac cgctctcgcc
     3841 gtggatgtgc aacttcacgg ggaccggctt cgcggatgtc tgctcgacca cccgaatctc
     3901 gacgcgccgg gtagcgggcg tcacggcgat caggggtgtc gaggcactcc gcgcttcgag
     3961 cgaagagacc ggtacggtct tgccgtccca caggtgaaat cgggcgtcgg gatgcgccgt
     4021 atactcgatg attacgtccc gcgccgaaat ctcgggcact ctgttgttgt atgtggcgga
     4081 ccagtcttcg ttgggataga ggctgcgcgg gacggcggaa atcacttggc ccatgtcgag
     4141 ttggatctgt ttcgagaggc cgcgcgaatc gatcgtgtag tcaaaccggg caccctcggg
     4201 cagcgtaaga atggctttct tccgcgtgcc ccaccgaagc ggctccgacg tcgcactgcc
     4261 ggcggagacc gcggacagaa cggtcagtcc cgacttcggc tcgaaccgga gggcagcgat
     4321 ctccttttcc gggtgagggt tcttccaggc ccagagccag ttgacccaag tgtcccggtc
     4381 cgctgagtcc gcgcgggttt cggccgagcc ccagccaagc tctccgccgg catcgaccag
     4441 catggaaggc tgctcgtgca ccggccggat ggcgcgcggc ttgttatgag caaccgccag
     4501 aaagcagttt tcgccccaca tcggctggaa cgcgccgagt tggtgacgcc tgcgaatctt
     4561 ctcgcgcact tcggtaccgt cggtgtagac gatcatgtag tcggccgcat gctcggcgag
     4621 gaatccttct ccgcgcatcg gccggatgag tccgcgctga tccttttcga cgggtctgga
     4681 gtcggctgta tggagaaata cgagccactc ggccttcaat cccggcgtct tctccgtaac
     4741 gggaccgtcc ttcagcacca caggacggtc gatgcggaac gggattcccc atgagacggt
     4801 ggccccgcgc ggcgccttgg aagccgcctt cgccatctct tccgacagtc cggcctgagc
     4861 cagagcgtcg atgttcgtgt tacccgcgag cgcgacgggc atgaagcctg tcgacgcggg
     4921 accatccccg gcgacatccg cggactccgc catcccaacc accccggccg cagaaaccac
     4981 cgccgccgat tccagaaaat gcctccggga aactgtcatc atgaacctcc ttgcactccg
     5041 ctcaatcaca ctcgacggag aggcagtata cacgctagga gagtcccttg tcctccatcg
     5101 ggcttaagag tgttccctcc gaaatcggta tataattccc ccgaaatccg ttttccggag
     5161 gtgatcatgc ccgacaattc tttctctttg acacgccggc agttcggcct ttcgctgggc
     5221 gcctcgacgg tagcggcggc gaacgccgtg ccgcctacgc tgccagagcc ctggatgggt
     5281 ccggccatcg tgaaaaaggt gtatctggcc aagccaaacc ccacgtggcc gcggcccgac
     5341 ctcaacattc agcaggagat cgctgcgatt gagccgcatc tctccgagct tgagcgaaag
     5401 catcccggcc gcatccgttt caccggcggc gatttgctca aagtcggaga cgacatctcc
     5461 ggttgggcgc gaagcctcga aggtgcggac gccatcctcg tcatcgacct tacctccggc
     5521 cttggcccgc tactccagaa gatgcgcgac atcgaagtgc ccatgctgct cttcacccga
     5581 ccgtatgccg gctgggccta catggacttc accgcctgga ggcagagcgg caagcgcgtt
     5641 gagcttgtcg ccagcagcga gttcggcgac ctcgacccct acatgcgcat ctttcagacc
     5701 attcaccacc tcaagcggag caaggtgctc atggtcgccc ctggcgacgg ctggcggcaa
     5761 acggcagcgg cttacacccg gcactacggc accaccattg agttcgtcag ttaccaggac
     5821 ctgaaagccg ctttcgacgc cgccgacgtc tcaaaggcga aacaggaagc cgccgagttc
     5881 acccgtcgcg catcccgcgt cgtcgagccg tcgccacagg aggtactcga cgccatgcgc
     5941 ttctacctcg gtgtcaagaa catcctggag cgagccaagg caaatgcgat caccgttgac
     6001 tgccttggcg gattccgccg cggcgacctt cccgcttacc cctgcgtcgc atggtccaag
     6061 ctgaacgatg agggcttcta tggcgtctgc gaggccgact tggcctccac aatgacacag
     6121 cttctcctga cgtccttctc cggaaagccc tgttttgtct ccgacccggt cttcgacacc
     6181 agccgcagcg agatcatcca cgcccattgc gtttccgcca ccgccatgca aggccttggc
     6241 ggtcctggtc ttccctattc cgttcgctcg cacatggagg acaacaaagg cgtctccatg
     6301 caagtgctca tgcccgtcgg tgatacggtc accgtcgcca agtacgacgg accaacaaaa
     6361 cttctcgtct ccaccggcga ggtcctcggc aacgtggaca acccgcgtgg ctgccgcaca
     6421 aagttccgca cccgtgtccc cggcgcgcgg aagatgctcg aaaactacac cgccggtctg
     6481 caccgtgtcg tggtctgggg cgactatact gaacccatcg aatccatgag gcgcctcatg
     6541 ggcttcgaca tcgtccgcga ggcgtaatgc catgtctctg tcgcgccgcc acttcctatt
     6601 ggccggaccg ctccttcctg ctctcagagg agcggccccg gccggcctgc gcgagtccga
     6661 gccggtcccg gaaccccatt tcccgagccg cctttaccag ttcgtctggc gaaactggga
     6721 actcgcgaat acggaccgca tggcggaggt tgcgggcgcg acgcctcggc aaatcctcga
     6781 cctcggcgag tcgatgggtc tccctgcgaa gccatccctt tcggaggacc agcttcgccg
     6841 catctacatc accgtgatcc gccagaactg gcacctgctt ccagagccac agatcataaa
     6901 gctcctcgga tggtccgatg agcgcttccg cttcaccctc aaggaagacg acttcctcga
     6961 tatcaagctc ggcctcaaca agccccgctg tccggagctg cgctacgatc ctccctcgga
     7021 ggccgaccaa cacgccgcgg cccgcatccg tgagacgctg cgggcagtgt tcggcgattc
     7081 gctccgcgct cccggtcagc gctctttcca gtttgtcgag gagttgagcg acctccgctt
     7141 cgtcccgatg cgggacccgt cggccaggcc cgctccgact gagatcgacc tcacctcggg
     7201 ttggagcatc gccgagccgg aaggcgacgt gctcgcgtcc gcggcacgcc gccttcgcga
     7261 gtttctccgg gacgcgatgg gagtttcgac cgctaccggc ggcaagcaca tcgtcctcga
     7321 agtcaggccg cccggcaacg gcccagggag cttcgacatc gccgtttcgg aagccgccgt
     7381 ccgcataacc ggcgacagcg attcgggtgc gctacagggc gtctactggc tcatggatca
     7441 gatggaggag cgcgaggcgc cattccttcg acccggtgtg acgtcgcgta aagctgtctg
     7501 gaacccgcgt tttctctact ctcacttcgc cctttatggc gacccgctga tggagccgga
     7561 agccgatccc ttccccgacg cttatctgga gaaactggcc cgccgcggca tcaatggcgt
     7621 ctggatgcaa gcggtgctga acaacctggc gccctcgaag cagttccccg agtttggcga
     7681 gggctcggag acgcgcctcc gcaatcttga cgcgctggtc cgccgcgccg cccgcttcgg
     7741 catcagcatc tacctctacc tcaacgaacc gcgcgccatg ccgccagagt tcttccgcgc
     7801 gcacccggag actcgcggtt catccttcct tgggacaaac gcaatgtgca cgagcgtgcc
     7861 tgccgttcgc gagtggatcg cggacgcgct gacgcacgtg gtccgccacg cacctggtat
     7921 cggtggcttc ttctccatca ccatgtcgga gaatcacacc aactgcttct cccatggcgg
     7981 cgcgtggggc aaagcagccc ccaacgccgg cgattgcccc cgctgcgcgc agcgcaagag
     8041 ctgggacgtc attgccgagc tgatcacgac gttccgtgac ggcgtccgcc gaggcggctc
     8101 ctccgcccac gtcatcgcct gggattgggg ttggggagac gacctgaccg cgaacctcat
     8161 cccactgctt cccaaggatg tgcgcttcca gagcatcagc gaatgggacc agccggtcaa
     8221 ccgcggcggc gtgccgatgc gcgtcggcga gtactcgatc tcggtcgtag gccccggtcc
     8281 gcgcgccacg cgcaactggg agcgtgcccg caagagcggc gtaaccgcga tggcgaagac
     8341 ccagttcaac aacacgtggg agatctccgc ggctccgtgg attcccgccg tgcacctcat
     8401 cgcccgccac tgcgaaaatc tcaccaaagc gggcatcgca ggaatcatgg cgagctggac
     8461 ctgcggcggc tatccgtcac ccaacctgtc tgcggccaag gcattctact tcgaaccgcg
     8521 cgagtccacg ccggccgtgc ttcgatccgt ggccgcgcgc cgttacggac gggcggcggc
     8581 cggggaggcc gtcgaggcgt ggcgccgctt cagcgaggcg ttcgaggagt atccgtacgg
     8641 cgtcgccgtc tacatcatcc cgacccagca cggccccgcg aacttgctgc gcctggagcc
     8701 aaccggccat gcacccggca tgatcctctt ccctcacgac gattacaaag cgtggtgcgg
     8761 aaagtaccca cccgaggttg tacagcggca gtttgggaag atggcggcgc tgtggaacga
     8821 gggtcttact gccttccgcg acgcgctagc gaaggtctca ccgcgcaaga aagccaacgc
     8881 gagcgccgac ctcgccgtgg ccgagacctg ctaccatcac ttccgcagca cggccaacca
     8941 ggtggagttc tacattctgc gcgaccggct acgctccgtc tcagacgccg agcgagcgca
     9001 gatcaccgcc cgcatgagag cgatcgtgga ggatgagatc gacctcgccc ggcgccagta
     9061 ctgggttgcc cgcgccaact ccgccatcgc ctacgaggcg tccaaccatt actactaccg
     9121 gccgctggat ctggtggaga aggtgctcaa ctgccgtgac atcctggccc ggttgaagtg
     9181 acgcctaccg cgcctcgacc gcaaatgtgc cgaagtatcc gctgatccca ggttcgttca
     9241 tgttccactg cttgtcgaac tcgtgcccct ggtagcgcgt gttcactgtc atgccgagcg
     9301 tcgactcctt cattggaatg aatggatcgt accagagcac cgccccttgc aacgcgtctt
     9361 gcagcggctt tgccttcctc agttcgacgt tcacgagatc gccgattcgc agggtcgcgt
     9421 gttccacgtc tctacggaat tcgatagggg ttgcctgaac actcagaatg ttgccaagaa
     9481 gactgccgtg cttctcccgc agcagccgtt ccaccgcctg ccgctggtcc ccgcttgcat
     9541 ggttgtctat gaagaggata ctcttgcgcg gagcgtctcc cgcgctcaac gacgcctcgc
     9601 ccatcaccac cgctgccgca gtcactcctg acaaagaagc gccagcgtac tcgcccgagt
     9661 ggaagcgcca tccgagaatc gcttccttgc cgccggtgac ccgttcaccc gaccactcgc
     9721 aatagcagcc gaacacccgg ttcgcccggt cctcgatgta ctcgcctgtg atgactggac
     9781 cctgcgccgc cgcaaacgcc cccgccaaca gggaaagcac cgcaaccaca ccatactttc
     9841 gcatggcctg atccctctgg ccttact
//
LOCUS       DATIFQ010000232         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2566112, whole genome shotgun sequence.
ACCESSION   DATIFQ010000232 DATIFQ010000000
VERSION     DATIFQ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2566112"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VLE22_10775"
     CDS             complement(<1..677)
                     /locus_tag="VLE22_10775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSB14933.1"
                     /translation="MTGRAISHYQILEKLGEGGMGVVYKARDTHLDRFVALKVLPPEK
                     VADPERKRRFVQEAKAASALNHPNIITIHDIDQVDGIDFISMELVAGKTLDRLIPRHG
                     MRLNEVLKCAVQIADAVAAAHGAGIVHRDLKPGNVMVTDKGQVKILDFGLAKLTDTAD
                     GSDELEMRTLRDGDAPRTVEGRIVGTVAYMSPEQAQGKKVDARSDIFSFGSLLYEMVT
                     GHLPFDA"
     gene            1014..1781
                     /locus_tag="VLE22_10780"
     CDS             1014..1781
                     /locus_tag="VLE22_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14934.1"
                     /translation="MNRVSKRHIAASFMLLAAHCFGETPSRETIVPLPRIGTRVYNIA
                     RVEEKLPQKVKEDRTTPRITVYVYNYAKVPITKLEEARREVILIYREAGIEAEWVDCP
                     CAAEEVNTYPDCLPGRLSKDWVAIRIVSQLMIDRWRRSSSTFGVALYSEHGEFGRFAS
                     VCAECAEQVVKVEPRRYGPLLGNLIAHETGHLLLATLRHSSFGVMRASWNQADLEDCG
                     RLRMFFSGEEARRIRDQVRERVRAQETVNAASLHSPK"
     gene            complement(1919..>2599)
                     /locus_tag="VLE22_10785"
     CDS             complement(1919..>2599)
                     /locus_tag="VLE22_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14935.1"
                     /translation="DADGSNAMQLTSVGGRTVFGSTWSPDSQKIAFYASIGGNTDIYV
                     ISANGGTPRRLTTEPGIDHWPCWSHDGQWLYFSSDRGGTDEIWRMPASGGKAVQVTQN
                     ANGADLPHESPDGKFVYYSKGWPFPQSVWRVPAEGGEKTKVLDAVHPYGLWTVGKEGI
                     YFFTVPDKKRHSDLSIYEFATGKTRKILTIERRVATFIAVSPDGRTILYPQVDEAGSD
                     LMLVENFR"
BASE COUNT          503 a          777 c          784 g          535 t
ORIGIN      
        1 tcggcatcga acggcaggtg gcctgtcacc atctcgtaca acaacgatcc gaaactgaag
       61 atgtccgacc tcgcgtcaac cttcttgcct tgcgcctgct ccggggacat ataggcgacc
      121 gtacccacaa tccggccttc cacggtgcgc ggcgcgtccc cgtcacggag cgttcgcatt
      181 tcaagctcgt cgctgccgtc ggctgtgtcc gtcagcttgg ccagcccgaa gtcgagtatc
      241 ttgacctggc ctttgtccgt caccatgacg tttccgggct tcaggtcgcg gtgtacgatg
      301 ccggctccgt gcgcagccgc cactgcgtcg gcgatctgca cggcgcactt gagtacttcg
      361 ttgagccgca tcccgtgccg ggggatcagc cggtccagcg tcttgccggc gaccaattcc
      421 atcgagatga aatcgatccc gtcaacctgg tcgatgtcat ggatggtgat gatgttcgga
      481 tgattcagtg ccgaggctgc cttggcttcc tgcacgaacc gccgcttgcg ctccgggtcg
      541 gcgaccttct ccggcggcag gaccttgagg gcgacgaaac ggtcgaggtg ggtgtcgcgc
      601 gccttataaa ccacacccat cccgccctcg ccgagcttct cgaggatctg gtagtgcgat
      661 atggcgcgcc cggtcatacc cggctccaaa ttcggaaaac ggaatcggga tatctctgtc
      721 ggatcgattg taccgccgtc cggatcggcc ttagacgaaa aaagcgcacc acgagcggcc
      781 acgccctccg gtgaacgggt gtagctcgct ggcccgatta caacaccgat tcctaagacc
      841 gccggttctt catccgcagg tgatgcaaaa gtataacctt cattgaatca atgggttgcg
      901 aggagagtaa cgtggggcta gcgagagatg aacgtaggag cgtcggcgca ttgagctacc
      961 ggcgctccgg accaatactt ggctcaccac gctaacgcgc gcaggtgagc caaatgaacc
     1021 gggtttcgaa aagacacatt gcagcgagct tcatgctgct ggcggcgcac tgcttcggcg
     1081 agacgccgtc cagggagaca attgtacccc taccgagaat cgggactcgg gtctacaaca
     1141 tcgctcgggt cgaggaaaag ctgccacaaa aagtaaaaga agatcgcacg acgccgagga
     1201 tcacggtgta cgtttataac tacgcgaagg ttcccatcac aaagctggaa gaagccaggc
     1261 gcgaggtgat actgatctac cgagaagccg gtatcgaggc ggaatgggtg gattgccctt
     1321 gcgctgccga ggaggtgaat acgtacccgg attgcctgcc aggcaggctg agcaaagact
     1381 gggtggcgat acggattgtt tcccagctca tgattgaccg ctggcggcgc tcgtcctcga
     1441 cgttcggcgt ggcgttgtac agtgaacatg gggaattcgg gcgcttcgct tcggtctgtg
     1501 cggagtgcgc ggaacaggtg gtgaaggttg agccgcgccg ttacggaccg ctcttgggta
     1561 acctgatcgc acacgagact ggccaccttt tgcttgctac gctgcggcac tcttcgttcg
     1621 gcgtcatgcg tgcgagctgg aatcaggcgg acttggaaga ttgtggccgc ctacggatgt
     1681 tcttcagcgg ggaagaagcc cgtcgaatcc gtgaccaggt gcgcgaacgc gtgcgggcac
     1741 aagagacggt gaacgcggcc agccttcatt caccgaaata gccccggccg ggctgtgaag
     1801 ctcaacggaa gtcctccatt cgcatcgggg cgctgctcat ctctacaagg actgcccccc
     1861 cacgctggcc tgaccggata gcggaagcct acgggcgttg tgtctagcca tctgggcgct
     1921 atctgaagtt ctccaccagc atcaggtcgc tgccggcctc gtcgacctgt gggtagagaa
     1981 tcgtccggcc gtcgggagag accgcgatga aggtcgcgac ccgtcgctcg atggtcagga
     2041 tcttccgggt cttgcctgtg gcgaattcgt agatcgagag gtcactgtgc cttttcttgt
     2101 cgggtaccgt gaagaagtag atcccctctt tgcccacggt ccacaggcca tagggatgca
     2161 ccgcatcgag aaccttggtt ttttcacccc cttcggcggg cacccgccag acactttgcg
     2221 ggaacggcca tcccttgctg tagtacacga actttccgtc gggggattcg tgtggcaggt
     2281 ccgctccatt cgcattctgg gtgacttgca ccgccttacc cccgctggca ggcatcctcc
     2341 aaatctcgtc agttccaccg cggtccgagc tgaagtagag ccattggccg tcgtgagacc
     2401 agcacggcca atggtcgatg cccggctcgg tcgtcagacg ccgtggcgtg ccgccgttcg
     2461 cgctaataac ataaatgtcg gtgttgcctc cgatgcttgc ataaaaggca atcttttgac
     2521 tgtccggcga ccacgtcgac ccaaacactg ttcgtcctcc aacggaagtc aattgcatgg
     2581 cattggatcc gtcggcgtc
//
LOCUS       DATIFQ010000233        24516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_458344, whole genome shotgun sequence.
ACCESSION   DATIFQ010000233 DATIFQ010000000
VERSION     DATIFQ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24516
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458344"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /gene="gnd"
                     /locus_tag="VLE22_10790"
     CDS             complement(<1..1169)
                     /gene="gnd"
                     /locus_tag="VLE22_10790"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HSB14936.1"
                     /translation="MEPKGDIALIGLAVMGQNLILNMNDHGYTVVAYNRTVSKVDEFL
                     NDAAKGRDTIIGAHSIEEMVKLLKRPRKIMLMVKAGKPVDDFIETLLPYLEAGDLIID
                     GGNSHFPDTMRRTTYLEGKGLLYIGTGVSGGEEGARFGPSIMPGGSPAAWPLVKDIFQ
                     AICAKTPDGEPCCDWIGEDGAGHYVKMTHNGIEYGDMQLICEAYQVMKVGLGLTNQEM
                     HEVFAEWNKGELDSYLIEITRDILGYTDENGEQVVDKILDTAGQKGTGKWTTVSSLDL
                     GIPVTLIGEAVYARCLSAMKDERVAASKTLKGPKTKFRGDRKAFVEDIRRALLASKIV
                     SYAQGFMLMREAAKEYKWNLNYGGIALVWRGGCIIRSAFLGKIKEAFDRDKNLSN"
     gene            1297..2736
                     /gene="tilS"
                     /locus_tag="VLE22_10795"
     CDS             1297..2736
                     /gene="tilS"
                     /locus_tag="VLE22_10795"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HSB14937.1"
                     /translation="MSVSSLPERVAETIERGSLFLPAHRVGVAVSGGADSVCLLHVLL
                     ELAPRWALRLSVLHLDHRLRDEESRLDAEFVRAMAARLGLPFCSESADVRRICEERGE
                     NLEQAARQVRRNFFLRYLREDVLDRVALGHTRSDQAETVLFRLLRGAGTTGLRGILPV
                     TAEGFVRPLLDVDRAEVEQFLAGRGIPWREDSSNRSLVFARNRIRQELLPSLARDWNP
                     ALAENLARMALLAREDEAYWRLEIDRIAAKCFVEKPPAVLLRVVDLACMDQAVRRRLI
                     RRAFELVKGDLRRIDFVHIEKVLSLTTAGEGDGRVQVPSVDVLRSFDWLRFAPAGEYG
                     ASERDFSILVAAPGRFRTPEGGEISLQLLEKSDGWESPDSGYNESGDLDWNRIRGPLE
                     LRNWRPGDEYRPSGHARGERIKFFFRQTRIPLWERRGWPILIGGGTIVWARRFGPAAE
                     FAATRESRMVLRVTETEDSADSGNLTDAR"
     gene            2800..4713
                     /gene="ftsH"
                     /locus_tag="VLE22_10800"
     CDS             2800..4713
                     /gene="ftsH"
                     /locus_tag="VLE22_10800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSB14938.1"
                     /translation="MNSNVKTAIFWVVLISVAVLLWIVVQTGKTRQDRQLTFSQFLGQ
                     VEAGKVKKVAITGNEVRGEFRDEAVGLRTTIPSNYPDIYNLLRDKNVDVDIKETSSGN
                     WISILINAVPFILLLAFWIFMMRQMQSGGNKALSFGKSRARLHSSQQKKVTFKDVAGV
                     EEAKEELQEIIEFLREPQKFQKLGGRIPKGVLLIGSPGTGKTLLARAIAGEANVPFFS
                     ISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQ
                     TLNQLLVEMDGFESNEGVILMAATNRPDVLDPALLRPGRFDRRIVVGRPDVKGREAIL
                     KVHTKKIPLADDVDLSVIARGTPGFAGADLANLVNEAALLAARQNRKVVTQYDFEVAK
                     DKVLMGVERKSLIISEEEKRNTAYHEAGHALVAAMTPGADPVHKVTIIPRGMALGVTM
                     QLPMDDKHSYTREYLEAQLTILMAGRIAEEKFMNHITTGAGNDIERATDMARRMVCEW
                     GMSEMGPLSFGKKEEQIFLGREIAQHRDYSEDTAVKIDDQVRKLVETSYERSKKTIDD
                     NADALVRIAEALLEREVLDGQEVRQLIEGVTLPIRVTTSGERDDAGSQPVVRPEGGHR
                     VPGLREGERPQPA"
     gene            4710..5345
                     /locus_tag="VLE22_10805"
     CDS             4710..5345
                     /locus_tag="VLE22_10805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSB14939.1"
                     /translation="MIPRYSFYLFDVDGTLLDSATDICGAVKEALAVTSADRVPESLL
                     RTYIGRHLYDLFEDLIPGISQIRMEELLQQYRAIYHARGHRGTRLYPGVIDALSRLGG
                     QKSTATTKSTQTTRLVLEKFGLLPYFDHVQGTDGFPSKPAPDVILRSLEALDAPPEEC
                     LLVGDSGPDMEAGRQAGVRTCAVRYGYGNREEMARWEPDYWIDDLRELNGT"
     gene            5407..6297
                     /locus_tag="VLE22_10810"
     CDS             5407..6297
                     /locus_tag="VLE22_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB14940.1"
                     /translation="MQRRQFLKNASAAAGFAAVSPVSLPLEAAQKKFRISLAQWSLHK
                     AIFARMTTNLEFPRIAREQFGIEGLEFVNGLWEAPTQDYVRELKQNMDKTGTKGVLIM
                     CDGEGLMGHSNKAERMRAARNHYKWVDIAAELGCHAIRTNMYPDKQPTAPAEIDAFIG
                     CCSESFANLCEYAKPMNISVIIENHGGVSSNPDVVNRLMKKVSLPNFGTLPDFGNFPK
                     EIDKYDAVRKLMPFAKGVSFKCYDFGADGNETTIDMHRMMKIVDEAGYHGWVGIEYEG
                     ERLTEFEGIQAAKRFLERVG"
     gene            6372..7475
                     /locus_tag="VLE22_10815"
     CDS             6372..7475
                     /locus_tag="VLE22_10815"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB14941.1"
                     /translation="MLWWTLRQLKSDDWGVAQRAAVELGTAKETKAVEPLLEALRRPY
                     EALQYAAAGALAEIGDPRAVPVLVAAFRQRPAEGFARALAKFKDRRAIPTLMGALMHQ
                     DSAVRDVARRALDEIAPNWPKSDEAKSALPALLEAIRDGGPDVKRVALDLVEEIGETA
                     TAIDALLDLVGRDVNSVGWVVRTLRDLDPGWTQTSRGKAAVPALVAVLKGQGYERDLA
                     AGTLAEIGDREAVGPLIDALRDRDRDLRNAAARALGRLGDPRAIGPIARAPLDNSLDP
                     WIAQQALNGIDPRWAATDYARTIISPCLEKVTDKDRGARDYAESTLNTIDPNWMRSAE
                     ALDAIAYFRQRSMHADPEISAAATRVLGLLGTS"
     gene            complement(7490..8194)
                     /locus_tag="VLE22_10820"
     CDS             complement(7490..8194)
                     /locus_tag="VLE22_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HSB14942.1"
                     /translation="MIIPCIDLMDGKVVQLVQGRDKALEGGTPQEMLDRFSAFPEIQV
                     IDLDAAIGKGSNDNIVEYLASRAATRIGGGVRTTERAVRLVEQGARKVIVGTSSFTTD
                     GVNEPFLRSVAAAIGKERLVIALDSKGGRIVVKGWRESLSLTAEEVIHRLEPFCAGFL
                     CTYVDKEGMLQGTDLDWFRRLRAATDIEITAAGGITTVDEIHELQRLNIHAALGMAIY
                     TGKLDLEELVRLSHNN"
     gene            complement(8191..8952)
                     /gene="hisF"
                     /locus_tag="VLE22_10825"
     CDS             complement(8191..8952)
                     /gene="hisF"
                     /locus_tag="VLE22_10825"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HSB14943.1"
                     /translation="MLAKRIIPCLDVTAGRVVKGVNFVNLRDAGDPVELADRYNQQGA
                     DELVFLDITASSDERETMVDVVARTARKVFIPLTVGGGIRSVADARKILMAGADKVSV
                     NTAAVHRPELLTELSREFGSQAVVLAIDAKRRSPGAWNVYTRGGRVDEGIDAIEWAAR
                     GEALGAGEILLTSMDTDGVQQGFDCELTRASSRATHIPVIASGGAGSPDDFVCVLTGG
                     EADAALAASIFHYGTYTVDDLKQHLERYGVPVRRP"
     gene            complement(8957..9553)
                     /gene="hisH"
                     /locus_tag="VLE22_10830"
     CDS             complement(8957..9553)
                     /gene="hisH"
                     /locus_tag="VLE22_10830"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HSB14944.1"
                     /translation="MIAIFDYGAGNLRSVENTLAEIGAAYTLVRDASGLDRASKMILP
                     GVGHFGQMLRALDDMQVRGKFLECIGAGIPFLGICLGLQALFAGSEEEPGLQGFGIVP
                     GMVRRFPETARVPHMGWNDLVPVRESRLLAGIGPKPYVYFAHSYYAPVVPESAAACSY
                     SDIRYTAVLESANLFGVQFHPEKSGPLGLKIMKNFVEL"
     gene            complement(9550..10137)
                     /gene="hisB"
                     /locus_tag="VLE22_10835"
     CDS             complement(9550..10137)
                     /gene="hisB"
                     /locus_tag="VLE22_10835"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HSB14945.1"
                     /translation="MRSATVERITRETQIRGSLKIEGRGRYEVDTGVRFLDHMLELFA
                     KHGGFDLKLRAAGDLDVDQHHTVEDVGIVLGQLFSQALGDRRGVNRAGYFVMPMDETL
                     AVVAVDLGGRPALVYKDMVKVRLVGDLQAELLEDFFGGLANQAGANLHVKVLYGRSNH
                     HKVEAIFKCFARAMKFACSKDKRLRGELPSTKGLL"
     gene            complement(10134..10808)
                     /locus_tag="VLE22_10840"
     CDS             complement(10134..10808)
                     /locus_tag="VLE22_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSB14946.1"
                     /translation="MPDEVLIFDMDGVLVDVTASYRETIRRTVQYFTGREVSHEFIQD
                     WKNAGGWNNDWALSQKIIRDYGVNVDYDTVVTQFQTFFFGDGKDGLIFQERWIAKPGL
                     LERLGARYRLAIFTGRLRDEAQVTLDRFARTLPFDPVVGTDDVVHGKPAPDGLLKISD
                     ITGGARLRYVGDTVDDARSARAAGVPFIGIASPDNPRREELVALLEAENAVAVLNDIN
                     DLESVL"
     gene            complement(10808..11884)
                     /gene="hisC"
                     /locus_tag="VLE22_10845"
     CDS             complement(10808..11884)
                     /gene="hisC"
                     /locus_tag="VLE22_10845"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSB14947.1"
                     /translation="MADWLEIPAGVLRPREAVLRMAPYSPPTGGRAGKLRLDFNENTL
                     GCSPRVIEFLKQKLTEDQLAVYPEYVEAKKDLAGFFGVVEDQLLLTNGTDEAIQVIVN
                     TYVDNGDDVVLLRPSYAMYRFYAEVAGASIREVDYRPADLAFPLSELLDAIRPSTRAV
                     LIANPNNPTGTGLSLDGVVQILEKAPGAAVLIDEAYYEFCGVTALGMIGRFSNLLVSR
                     TFSKVYGMAAMRLGCVFSQEVNINYLHKAQSPYSVNMLAALAAREAVRDTEYISNYVS
                     EAISSRDLLYEGLERLGITYFHSKANFVLFRAGGRAIEIRDRLREMGVLVRDRSYELP
                     GCVRVTVGTREQTRRFLAALEEIW"
     gene            complement(11877..13127)
                     /gene="hisD"
                     /locus_tag="VLE22_10850"
     CDS             complement(11877..13127)
                     /gene="hisD"
                     /locus_tag="VLE22_10850"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HSB14948.1"
                     /translation="MIRIIDDKQADRFLQRRTARLTEAEQTVAPILEAVRKHGDRALL
                     KYVRKFDGLERGRLSVEVPASDTEAAYRGVTSAFRRAVRTAARNIRDFARMQLPVAKT
                     VRSRDGHRTGQIVRPLGAMAAYVPSGRYPLPSTLLMTVIPAQVAGVRRCCVASPRPSP
                     EVLGTARFLNVSSVYRMGGAHAIAAFAFGTETVPKVDRIVGPGNIYVAAAKKLLAGEV
                     GIDFIAGPTEILIVAADGDPRVIAADMLAQAEHDTDASAILVTTSKRLATAVAREVDR
                     QLADLPTAPTARVAIEKNSMIILVKSIERAMEISNCFAPEHLSIPDASLVKLVTSAGS
                     VFVGPHSPEAAGDYASGPNHVLPTSGAARVRAGLSSADFVKVISVQELGATSLCKLAP
                     AITTLARAEGLEAHARSVEVRIRG"
     gene            complement(13155..14024)
                     /gene="hisG"
                     /locus_tag="VLE22_10855"
     CDS             complement(13155..14024)
                     /gene="hisG"
                     /locus_tag="VLE22_10855"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HSB14949.1"
                     /translation="MKLKLGIPKGSLESATIDLFRRAGFSITTSSRSYFPAIDDPEIE
                     CMLIRAQEMARYVEDGVLDAGLTGHDWVMETEAKVRTIADLIYAKQSFGKVRWVLAVP
                     EASTFRSVKDLEGKVIATELVNATRRYLASHGVTAKVEFSWGATEVKPPVLADAIVEV
                     TETGSSLRANKLRIIETVLESNTQLIANLDSWCDEWKRRKLEDIRMLLEGAINALGKV
                     GLMLNVERKNLDAVLAVLPALKRPTISHLSDEEWLAVNTILDESTVRSIIPRLKEAGA
                     QGIVEYPLNKIVM"
     gene            complement(14021..14401)
                     /gene="hisI"
                     /locus_tag="VLE22_10860"
     CDS             complement(14021..14401)
                     /gene="hisI"
                     /locus_tag="VLE22_10860"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HSB14950.1"
                     /translation="MNLDFNKLGGLAPAVIQDHATGRVLMLGFMNEEAFRKTVETGFA
                     TFYSRSRNKLWLKGETSGHRLVVREISTDCDQDAVLVKVEALGPGVCHNGYRSCFYRT
                     LQNGQWMESEPPVYDPKQVYGGGQ"
     gene            14626..15441
                     /locus_tag="VLE22_10865"
     CDS             14626..15441
                     /locus_tag="VLE22_10865"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HSB14951.1"
                     /translation="MRRIIAAGLIFLCSSFLWAQQTAPPKIQVLIITGQNGHDWRSVT
                     PELRKALEDTGRFEVRVTEEFRDAGPQTLAPYDLVVLNYFERRQPDLWWGERAQNALL
                     DYVRSGKGLVMYHFSTAAFDGWTEYEKMSGGNWRPNNGHHSPSHDFTVDIKDSDHPIT
                     RGLKKSFPQPHDELYANLKWQPADTYHVLATAWDDHSLYGGKARQPTPGAGLDQPMLW
                     TLNYGKGRVFGTALGHDAPAVRTPAFVTTFTRGAEWVATGAVTLPVPPEMAAR"
     gene            complement(15465..16265)
                     /locus_tag="VLE22_10870"
     CDS             complement(15465..16265)
                     /locus_tag="VLE22_10870"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB14952.1"
                     /translation="MSANDGRWDRVAEAPAPSWYLDPLVARQKREVHQQLIRRWTEGC
                     PVESVLKTDLFEEAFGEDQILFDLFPGPHRVIGIDVSWPTAARALRRSPLGTAFVLAA
                     DVRSLPLRSDLVDVIVSNSTLDHFDTREEFQAALSELARVLRPGGVLVISIDNAYNPL
                     YQLLRLLSRLGVGPFPLGYTTSLRGLIQSLEKHGLEVAAKSRLIHNPRLISTLLFLGL
                     RGTLGKRADTPIRWLLGLFSRLDALPTWPLTACFVAACARKPAGPPVK"
     gene            16347..17270
                     /locus_tag="VLE22_10875"
     CDS             16347..17270
                     /locus_tag="VLE22_10875"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HSB14953.1"
                     /translation="MIGSSAAVLREKLNRRQALLVPGCHDALSARVIENAGFEAIQVS
                     GFGLAGSLLARPDVGLVEMKDVLEHTWHVVRAVGIPIMADIDTGGGNAMNAADITERL
                     IAMGIAGVNIEDQVFPKRCGHMEGKQVIGAEEMAGKVRAMADVRRRVGADIVINARTD
                     ACAVYGLEEAIRRANLYLEAGADMAFLDGIRTRTDVERAVREVKGLLSVNLMDAVTGV
                     KTELIPIPELARMGVARVSIPVASIMVVHRALKEFFDALRASPTGLLAGETQWLSSFA
                     EYTDFVGLNEYRRLENEYLPKATLEEKYSVR"
     gene            17290..18516
                     /locus_tag="VLE22_10880"
     CDS             17290..18516
                     /locus_tag="VLE22_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HSB14954.1"
                     /translation="MTIAEKILARASGREKVAPDEVVVAAVDLAMSHENADLVRKSFR
                     EIGVARVWDPKKIVIILDHRVPAESEKTATTHKAIREFVKEQEIENFYDVGRGGICHQ
                     VLAENGHVKPGMVVVGTDSHTTTHGAFGAFAAGIGATEMAGVWVEGRLWFKVPATIRI
                     EVSGELRSWVSAKDLILYVIGKLGADGADYRAVEFDGPAIRRLTIASRMVLANLSMEM
                     GAKVAFTPIDQIGPDADARYERVLTIDADSKVAEPQIACPHTVDNVRPLSELGEVPVD
                     QAVLGSCTNGRLEDLEMAARILAGRRVHARTRLLVIPASQRIYLEAMRLGYLQTMIEA
                     GAMINPPGCGPCVGVHQGILAADEVCVSSTNRNFVGRMGSKDSQVYLASPAVVAASAV
                     AGRIIHPAEIVEEVCA"
     gene            18513..19022
                     /locus_tag="VLE22_10885"
     CDS             18513..19022
                     /locus_tag="VLE22_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HSB14955.1"
                     /translation="MSDVSMVLRGRVAAKLGDNVDTDVIYPGRYLNITDREKTVEHLF
                     ELTYPEIRSGIRPGDFLVGGKNFGCGSSREQATAAVKFAGVGAVIAVSFARIFFRNSI
                     NLGLPVVVSPAALEIEAGDELEIDLASGSIRNLTHGTAITAAPLDPRAVELLEAGGLI
                     PYLKKKYAA"
     gene            19066..20190
                     /locus_tag="VLE22_10890"
     CDS             19066..20190
                     /locus_tag="VLE22_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HSB14956.1"
                     /translation="MPKYRIAWLPGDGIGPDVCDAARLVLDAVGFEAEYIHGDIGWEF
                     WKTEGDALPQRTVDLLRSTDCAFFGAITSKPVAEAERELASALRGSGLTYRSPIVRMR
                     QLLDLYICLRPCKAFPGNPLNYREDIDLVVFRENTEGMYIGVEFDPVPVKFYDEPAMK
                     KIPRDAAISVRSITRDGSRRIVEAAFEHAVKNGRSKVTAVHKANVLRSTCGLFLDVAR
                     EVAARYPQVGFDHANIDAICMWLLKNPRNYEVIVTTNLFGDILSDLCAQLVGGMGFAY
                     SGNIGRNYAVFEPTHGSAPKYAGQNKVNPLATILAAKMMLGWLGERQKAMEIELAVAD
                     VVERGNVRTYDMGGSATTEDMARAVVQTLTFGQAVVDSER"
     gene            20198..21790
                     /locus_tag="VLE22_10895"
     CDS             20198..21790
                     /locus_tag="VLE22_10895"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HSB14957.1"
                     /translation="MEMRLIREIADKLQIPDPYLEYYGKYTAKLRLELLGDAIPPRHG
                     KLILVTAITPTSHGEGKTVVSIGLAQAIERLGRRSLVTLREPSLGPVFGLKGGATGGG
                     KAQVLPSEIINLHFNGDFHAVTAAHNLLAAMIDSHIHNSNQLQIDVDNIFWPRAIDMN
                     DRALRHIIVGLGGKVNGVPRETGFVITAASEVMAILALANSRQDLRRRLGEIVIGFDL
                     NGKVLRAGDLGATGALMVLLNEAIMPNLVQTTEHTPALVHAGPFANIAHGTSSVISQK
                     MALKLAEYVINETGFGADLGAEKYFDIVMPASGLKPSACVLIATVRALQAQGGFDNLA
                     RHLENLAKFDLPVVVAVNRFYSDSAADIAAVREFCRASGVECAETDVYSDGGKGGLEL
                     AEKVMTAAAAGDPARVRPLYVPDLGIEQKVETIARGIYGADGVSFELSARKKIRKFAE
                     LGFSRLPVCVAKTQSSLSDNPRLLGRPTGWTLMITDAHLAAGAGFIVVVAGNMLLMPG
                     LGKDPQAARMDVDEDGRILGLV"
     gene            21856..22755
                     /locus_tag="VLE22_10900"
     CDS             21856..22755
                     /locus_tag="VLE22_10900"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB14958.1"
                     /translation="MIRRSFLAGLAGALPVAVAAATEQSNRPRFKLRYGPHPGMFKNS
                     AGEDIIDQIKFAADQGFTGWEDNGAISRPPELQFRIGSALQQLGMTMGVFVSLADFRT
                     SDFVTKTDKPYQDSLRDMAKRAVETAKRVGTKWTTVVPSTVNNSLDPGFQTANCITNL
                     KVMAEVCEPAGLIMVLEPLNWYANHPGLFLRSVPQGYAICKAVDSPSCKILDDLYHQQ
                     VDVGNLIPNMEKAWDEIAYIQVGDNPGRKEPTTGEINYRNIFRWLHARRYDGVVGMEH
                     GLSKPGKEGEMALIAAYRECDSY"
     gene            complement(22756..23733)
                     /locus_tag="VLE22_10905"
     CDS             complement(22756..23733)
                     /locus_tag="VLE22_10905"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HSB14959.1"
                     /translation="MERADARGVASRLFYVRRLTVLLIIWLAHGFLLWRGDILAPYAL
                     MGIFLLLFRKRSQSTILIWAVIFYAWPMMLMGAAMAAHSAGAVIPTPPRPTPEELQRI
                     IQIFANGTYAEMLRVRVKDMAFNGIGLVFFYPRVLGLFLFGLWVWRTGIIRSPAQYEP
                     VLRRCRNWGLVVGLVGNAAVVAMLEIWRPNPTEFTREGFLVGIAGSIAMPALSLFYAS
                     SGLLLSQEGTWRRVLRPFGAVGRTALSNYLLQSVVCTTLYNSWGFGLFGKVGPLAGLI
                     PTILIYSAQVPLSTWWTARFTFGPTEWIWRTLTYSRLQTLSRSAAVQTP"
     gene            complement(24091..>24516)
                     /locus_tag="VLE22_10910"
     CDS             complement(24091..>24516)
                     /locus_tag="VLE22_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HSB14960.1"
                     /translation="GLKTLPLRMERQCANACRVAARLADHPCVARVHFPGDPGHPDAA
                     TIRRLFPEGLYGAMVSFEMRDAARAEVFAFMERLKMIVPATSLGDVHTMVLYPSMSSH
                     REVSPKQRERMGIRDNLVRLSVGIEAIEDILEDLDQALS"
BASE COUNT         4856 a         7314 c         7729 g         4617 t
ORIGIN      
        1 aagttgctca ggtttttgtc gcggtcgaac gcttctttga tcttgccaag gaaggccgaa
       61 cggatgatgc agcctccgcg ccacacgagc gcaatgccgc cgtagttgag gttccactta
      121 tactctttcg ccgcctcgcg catcagcatg aatccctgcg cgtaggagac gatcttggag
      181 gcgagcagcg ccctgcggat atcctcgacg aatgccttcc ggtcgcctcg gaatttcgtt
      241 ttcgggccct tcagggtctt cgaagccgcc actcgctcat ccttcatcgc actcaggcag
      301 cgggcgtaga cggcctcgcc gatgagcgtc actgggattc cgagatcgag cgaactgacg
      361 gtcgtccact tgccggttcc cttttggccg gcggtgtcga ggatcttgtc gacgacttgc
      421 tcgccgttct cgtccgtgta accgaggatg tcacgggtga tctcgatcag gtagctgtca
      481 agttcgccct tgttccactc agcgaagact tcgtgcatct cctgattcgt gaggcccagt
      541 ccgaccttca tgacctgata ggcctcgcag atgagctgca tgtcgccata ctcgatgccg
      601 ttgtgggtca tcttgacgta gtggccggcg ccgtcttcgc cgatccagtc acagcacggc
      661 tcgccgtcgg gggtcttggc gcaaattgcc tggaaaatgt cctttactag cggccacgca
      721 gcgggactgc cgccgggcat gatcgacggg ccgaagcgcg cgccttcttc gcctccggaa
      781 acacccgtcc cgatgtacag gaggcccttg ccttcgagat acgtggtccg gcgcattgtg
      841 tcagggaagt gcgagtttcc gccatcgatg atcaggtcgc ccgcttcgag gtaaggcaga
      901 agcgtttcga tgaagtcatc gaccggcttg cccgccttga ccatcagcat gatcttcctt
      961 ggccgcttca gcagcttcac catctcctcg atcgagtgag cgccgataat cgtgtcgcgt
     1021 cccttcgctg cgtcattcag gaactcgtca actttcgaaa ctgttctgtt gtaagcgacg
     1081 accgtgtagc catggtcgtt catgttgagt atgaggttct ggcccatcac ggccagacca
     1141 attaatgcga tgtctccctt aggttccatg gttcgctcct aactgcgtgt tgaaacttcg
     1201 aactgtacgg tgagagtgcc ggagagggcc tagctcaagc gcgtcgcaac caatactcgt
     1261 tatagcatga atgacgccgg ttctagaatg gaggtagtgt ccgtctcctc actgccggaa
     1321 cgcgtcgccg aaaccatcga gcggggttcg ctttttctac cggcgcaccg cgtgggtgtg
     1381 gccgtttcag gcggcgcgga ttcggtttgc ctgttgcacg tgttgttgga actggctccg
     1441 cgatgggcgt tgcgactgag tgtgttgcat ctcgaccaca ggctgcggga cgaagaatcg
     1501 cgtttggatg cggagttcgt ccgcgcgatg gcggcgcggt tgggcttgcc gttctgctcg
     1561 gaatccgccg acgtccgccg gatatgcgag gagcgcggcg aaaacctgga acaggcggcg
     1621 cggcaagtcc gccgtaattt tttccttcgc tacctgcgcg aagacgtgct cgatcgtgtt
     1681 gcgctcggac atactcggtc ggaccaggcg gagactgttc ttttccgctt gctgcgcggc
     1741 gcgggtacca caggtctccg cggtatcctg cccgtcaccg cggagggctt cgtgcgcccc
     1801 ttgttggatg tagaccgggc cgaggtggaa cagttccttg ctggccgcgg catcccttgg
     1861 cgggaggatt cgtccaaccg aagcctggtg ttcgcgcgaa atcggatccg gcaagagctt
     1921 ttaccgtctc ttgctcgcga ctggaacccg gcactggcgg agaatctcgc ccggatggcg
     1981 ttgcttgcca gggaagacga ggcgtactgg cgcctagaga tcgaccggat cgcggcaaag
     2041 tgtttcgttg agaagccgcc ggcggtactg cttcgcgtcg tggaccttgc ctgtatggat
     2101 caagctgtcc gtcgccggct catacggagg gcattcgagc ttgtgaaggg ggacctccgg
     2161 cggatcgatt tcgtacacat tgagaaggtg ttatcactaa ctacggcagg cgagggcgat
     2221 gggcgcgtcc aagttccgag cgtggacgtt cttcgatcct tcgattggct tcgtttcgct
     2281 cccgcgggcg aatacggagc ctcggagcgt gacttttcga tcctggttgc cgccccgggg
     2341 cggttccgaa cacccgaagg tggggagatc tctctgcaac tacttgaaaa atcagatggc
     2401 tgggaaagcc cggattccgg ctataatgaa agtggagatc tcgattggaa tcggatccgg
     2461 gggccgctag aacttcggaa ctggaggccg ggggatgaat atcggccgtc agggcacgct
     2521 cgaggcgaac gaattaagtt tttttttcgg cagacgcgaa ttcctctctg ggaacggcga
     2581 ggctggccga ttctgattgg cgggggaacc attgtatggg caaggcgatt cggtccggca
     2641 gcggagtttg cggccacgcg ggagagccgg atggttctac gggtgacgga aacggaagat
     2701 tctgcggatt ccgggaatct cactgacgcg cggtgacgtc tctatgtatg tgatgagaag
     2761 aaaatgggat ctgcagcccc tcgccggtag ggaggcgtga tgaattcgaa cgtgaagaca
     2821 gcgatcttct gggtcgtgct gatcagcgtg gcagttcttc tctggattgt cgtgcagacc
     2881 gggaagaccc gacaggatcg gcaactcacc ttcagccagt tcctcggaca ggtggaagca
     2941 ggcaaggtca agaaggtcgc gatcactgga aacgaagttc ggggcgaatt ccgcgacgag
     3001 gcagtcgggc tgcgaacgac cattccgtcc aactatccgg acatctacaa tctcctgagg
     3061 gacaagaacg tcgacgtcga cattaaggaa accagttccg gaaactggat ttccattttg
     3121 atcaacgcgg taccgttcat acttctgctc gcgttctgga tcttcatgat gcggcagatg
     3181 caaagcggcg gaaacaaagc cctcagtttc ggcaagagcc gtgcccggct gcactcctcc
     3241 cagcagaaga aagtgacgtt caaagatgta gccggcgtgg aggaagcgaa ggaagaactc
     3301 caggagatca tcgagtttct gcgcgagccc cagaaattcc aaaaactggg cggcaggatt
     3361 ccgaagggag tactgctgat cggatcgccg ggtacaggga agacgttact agcaagggcc
     3421 atcgccggcg aagcgaacgt gccgttcttc tccatttccg ggtcggactt tgtggagatg
     3481 ttcgtcgggg tcggtgccag ccgcgtccgc gacctgttcg agcagggcaa gaagaatgct
     3541 ccgtgcatca tcttcatcga cgagatcgac gccgttgggc gacaccgtgg agcgggcttg
     3601 ggcggcgggc atgacgagcg cgaacagacg ctaaaccagt tgctggtgga aatggacggc
     3661 ttcgagtcga acgaaggcgt tattctgatg gcggcgacga accgcccgga cgtgctcgat
     3721 ccggcgctgc tccggcccgg gcgctttgac cgccggatcg tggtaggacg gcccgacgtg
     3781 aaggggcgcg aggccatcct caaggtccac accaagaaaa tccctctcgc cgacgacgtg
     3841 gatctttcgg tgatcgcgcg aggaacgccg ggcttcgcgg gagcggacct cgcaaacctg
     3901 gtgaacgagg cggcactgct agcggcgcgg cagaaccgga aagtagtaac ccaatacgat
     3961 ttcgaagtcg caaaagacaa ggttctcatg ggcgtggagc gcaagtcgct gatcatcagc
     4021 gaggaagaga agcgcaacac ggcttaccat gaggccggtc acgccctggt ggcagccatg
     4081 acgccgggcg ccgatccggt tcacaaggtg accatcatcc cgcggggcat ggcgctcggc
     4141 gtgacgatgc agttgccgat ggacgataag cattcctaca cgcgagagta cctggaagcg
     4201 cagcttacca tcctgatggc cggtcgcatt gccgaagaaa agttcatgaa ccatatcacc
     4261 accggcgccg gaaacgatat cgaacgcgcc accgatatgg cgaggcgaat ggtgtgcgag
     4321 tggggcatga gcgaaatggg tccgctgagc ttcggcaaga aggaagagca aatctttctc
     4381 ggccgggaaa tcgcccagca tcgcgactac agtgaggaca ccgctgttaa gatcgacgac
     4441 caagtccgga agcttgtgga gacgtcctac gaacggtcga agaagaccat cgatgacaac
     4501 gccgacgccc ttgtgcggat cgccgaggcg ctgctcgagc gcgaggtgct cgacggtcag
     4561 gaagtaagac agttgatcga gggcgtaaca ctccctatcc gcgttaccac ttccggtgaa
     4621 cgggatgatg ccggatctca gcctgtcgtg cggcccgagg gcggacatag ggtgcccgga
     4681 ttgagagaag gagagcgtcc tcaaccggcg tgattccccg gtattccttc tatctgttcg
     4741 acgtcgacgg cacgctgctc gactcggcga cagacatctg cggcgctgtc aaggaggcgc
     4801 tggctgttac ttccgccgac cgtgtgccgg agagtctgct tcgcacctac atcggccggc
     4861 acctctacga cctattcgaa gatctgattc ccggaatctc gcagatccgg atggaggaac
     4921 ttctgcagca gtaccgtgcg atctatcacg cccgcggaca tcgcggcacg cggctgtatc
     4981 cgggtgtgat cgacgcgctg tcgcgcctcg gcgggcagaa gtccaccgcg accaccaaga
     5041 gcacacaaac cacccgcctc gttctcgaga agtttgggct tttgccctat ttcgaccacg
     5101 tgcagggcac ggacggcttc ccgagcaagc ctgccccgga cgtaattctc cggtcgctcg
     5161 aagcgttaga tgcaccgccg gaagagtgcc tgctggttgg cgattccggt cccgacatgg
     5221 aagcggggcg gcaggcgggc gtgagaacgt gcgcagtccg gtacggttac ggcaatcggg
     5281 aggagatggc ccggtgggag cctgactact ggattgacga cctgcgggaa ttgaacggca
     5341 cgtaacgcgg gcccggggtc cggccgcggg gctctcgccc gtaaaccacc tgctatcata
     5401 ggcgtcatgc agcgacgcca attcctgaag aacgcttcag cggccgccgg gttcgcggcg
     5461 gtttcccctg tgtctctccc gctagaggcg gcgcagaaaa agttccggat ctcgcttgcg
     5521 caatggtcac ttcataaagc gatcttcgcg cggatgacga cgaatctcga gtttccgcgc
     5581 atcgcgcggg aacagttcgg gatcgaggga ctggaattcg tcaatggact gtgggaggcg
     5641 ccgacccagg actacgtccg cgagctcaaa caaaacatgg acaaaacggg aacgaagggc
     5701 gtgctgatca tgtgtgacgg cgaggggctg atgggtcact cgaacaaagc agagcgaatg
     5761 agagctgcca gaaaccatta caagtgggtg gacatcgccg ccgagttggg ctgtcatgca
     5821 attcggacga acatgtaccc ggacaagcag cccacggcgc cggctgaaat tgatgccttc
     5881 atcggttgct gctcggagag tttcgccaac ctgtgcgagt atgcgaagcc gatgaatatc
     5941 agcgtgatca tcgaaaacca cggaggagta tcgtccaatc ccgacgtggt caacaggctg
     6001 atgaagaagg tgagtctgcc gaatttcggg acgctgcccg atttcggcaa tttcccgaaa
     6061 gagatcgaca agtatgatgc agttcggaag ctgatgccgt tcgcgaaggg cgtgagtttc
     6121 aagtgttacg atttcggtgc cgatggaaat gaaaccacga tcgacatgca ccgcatgatg
     6181 aagattgtcg acgaggcggg gtaccacggt tgggttggga tcgagtacga aggtgagcgt
     6241 cttacggagt tcgaaggcat ccaggcggcg aagcgcttcc tggaacgggt agggtagacg
     6301 ggtcggagga gttccggagc acgggtcgtt cccggtttga accggccggc aatacgcgga
     6361 ggtggattgc catgctgtgg tggactctca ggcagttgaa atcggatgac tggggggtgg
     6421 cgcagcgcgc cgccgtcgaa ctcggaaccg caaaggaaac aaaggcggta gaacctctgc
     6481 tggaagcgtt gcggcggcca tacgaagcgc ttcaatacgc cgctgccggg gcgttggctg
     6541 aaatcggcga tccgcgtgcc gtccccgtcc ttgtggccgc gttccggcag cgcccagccg
     6601 aaggattcgc acgggcgctg gcgaagttca aggaccggcg cgccattccg accctgatgg
     6661 gcgccctgat gcaccaagac agcgccgtcc gcgatgttgc ccgcagggct ctggacgaaa
     6721 tcgccccgaa ctggccgaaa tccgacgagg cgaagtctgc ccttcccgct ctgcttgaag
     6781 ccattcggga tggcggaccc gacgtgaaga gggtggcgct cgatctggtg gaagagattg
     6841 gcgaaacggc gaccgccatt gacgccttac tcgacctggt cgggcgcgat gtgaattccg
     6901 tcggatgggt ggtccggacg ttgagggatc tggatcccgg atggactcaa acttctcggg
     6961 ggaaggcggc ggttccggcc ctggttgccg tgctgaaggg ccagggttac gagcgcgatc
     7021 tggcggcggg aacgcttgcc gagatcggcg atcgggaggc agtggggccg ctgatcgatg
     7081 ccctcaggga tagggaccgc gacctcagaa acgccgccgc ccgggcactc gggagactcg
     7141 gtgatccacg ggcgatcggg ccgattgccc gcgccccatt ggacaactcg cttgatccgt
     7201 ggatcgcaca gcaggcacta aacggcattg atccgagatg ggctgccacc gactatgccc
     7261 gaacgatcat ttccccgtgt ctggagaagg taaccgacaa ggatcggggg gcgcgggact
     7321 atgccgaatc gacgctgaac accatcgatc cgaactggat gcggtcggcg gaggccctcg
     7381 atgccatcgc ctatttccgg cagcgctcca tgcacgcgga tccggaaatc agcgcggccg
     7441 ctaccagggt actcggatta ttggggacga gttgacgtta agcgtcgcgt caattgttat
     7501 ggctgaggcg caccagttcc tcgaggtcta gcttccccgt atagatcgcc attccgaggg
     7561 cggcgtggat attcaggcgc tgcaattcat gaatttcgtc gaccgtggtg atgcccccgg
     7621 cagcagtgat ctcgatgtcc gtggcggcgc gcaagcggcg gaaccagtcg agatcggtgc
     7681 cctgtagcat tccttccttg tcgacatagg tgcacaagaa gccagcgcag aaaggctcca
     7741 gccgatggat gacctcctcg gccgtcagtg acagcgactc ccgccaaccc ttcaccacga
     7801 tccggcctcc cttggaatcg agggcgatta ctagccgctc tttgccgatc gcagcggcta
     7861 ccgaacgaag gaacggctca ttgacgccgt cagtggtgaa tgaggaggtg ccgacgatca
     7921 ctttgcgcgc gccctgttcg accagcctta cagcacgctc cgtagtgcgg acgcctccgc
     7981 cgatgcgggt tgccgcgcgg gaggcaaggt actcgacgat gttgtcgttc gagcctttgc
     8041 caatggcggc gtcgagatcg atgacctgaa tctcggggaa ggccgagaac cggtccagca
     8101 tctcctgcgg cgtccccccc tccaaggctt tgtcgcggcc ctggacgagt tggaccacct
     8161 taccgtccat caggtcgatg cacggaatga tcacgggcgc ctcaccggaa cgccgtatcg
     8221 ctccagatgc tgcttcaggt cgtccaccgt gtatgttccg tagtgaaaaa tggaggcggc
     8281 caacgcggca tccgcctcac ctccggtgag aacacagacg aaatcatcgg ggcttccggc
     8341 gccgcccgaa gcgatcaccg ggatatgcgt cgcccgtgag gaggcccgcg tgagttcgca
     8401 gtcgaaaccc tgctgcacac catcggtatc catcgaagtg agcagaattt cgccggcgcc
     8461 gagcgcctct ccgcgcgcgg cccactcgat ggcatcgatt ccttcatcga cgcggccgcc
     8521 gcgcgtgtac acattccacg cccccggcga acggcgcttt gcgtcgatcg caagcaccac
     8581 ggcctgcgag ccgaactcgc ggctcagttc ggtaagcaac tcagggcggt ggaccgcggc
     8641 cgtattcacg ctcaccttat cggccccggc catgaggatc ttccgggcgt cagcgaccga
     8701 acggataccg cccccgacgg tgagcgggat gaagactttg cgtgcggtcc gggcgacgac
     8761 atcgaccatc gtctcgcgct catcgctcga tgccgtgatg tcgaggaaga caagctcgtc
     8821 ggcgccctgc tggttgtacc ggtccgccag ttctaccgga tctccggcgt cccggagatt
     8881 cacaaaattg actcccttga caacacggcc ggcggtgacg tcaaggcacg gaataatgcg
     8941 tttggcgagc atggtgctac aactccacaa agttcttcat gatcttcagc ccgagcggtc
     9001 cggacttctc cgggtggaac tgaacgccga acaggttggc gctttcgagc accgcggtat
     9061 agcgtatgtc cgagtaggag caggcggcgg cgctctccgg cacgacgggt gcgtagtagc
     9121 tgtgggcgaa gtaaacgtac ggtttgggac ctatcccggc caacaggcgt gactcccgga
     9181 ccgggacgag atcgttccag cccatgtgag gcacccgggc ggtctccgga aaacgccgga
     9241 ccatgcctgg gacgataccg aagccctgca gcccgggctc ctcttcgctt ccggcaaata
     9301 gcgcctgcaa gcccaaacag attccgagaa acgggatacc ggctccgatg cactcgagga
     9361 actttccgcg cacctgcatg tcgtcaagcg cccgaagcat ctggccgaaa tggccgacgc
     9421 ctggcaggat cattttcgac gcccggtcga gccctgacgc gtcgcgcacc agcgtgtaag
     9481 ctgctccgat ctccgccagc gtgttttcga ccgatcgcag attgccggcc ccgtagtcga
     9541 agattgcgat cacagaagcc ccttcgtcga cggcaactcg ccgcgcagcc gcttatcctt
     9601 ggaacaggcg aacttcatgg cacgggcgaa gcacttgaag atcgcttcta ccttgtgatg
     9661 gttcgagcgg ccgtagagca ctttcacgtg gagattagcg cccgcctggt tggcgaggcc
     9721 cccaaagaag tcctcgagca attctgcttg aaggtctccc acgagccgga ccttcaccat
     9781 atccttatag acgagcgccg gccggccgcc gaggtcaacc gcaaccaccg ccagcgtttc
     9841 gtccatcggc atgacgaagt agccggcgcg gttgacgcca cgccgatcgc cgagcgcctg
     9901 gctgaacaac tggccgagca cgatgcccac atcttcgacc gtatggtgct ggtcgacgtc
     9961 gaggtcgccc gcggcacgaa gcttcaggtc aaagccgccg tgtttggcga acaactccag
    10021 catgtggtca agaaatcgga cccccgtgtc gacctcgtac cggcctcgtc cctcaatttt
    10081 caggctgccg cggatctgtg tttcgcgggt gatgcgttcg acggtagcgg atctcacagc
    10141 acgctctcca gatcgttgat gtcgttcagc accgcaacgg cgttttctgc ctcaagcagc
    10201 gccacgagct cctcgcggcg ggggttgtcc ggtgaagcga ttccgatgaa cgggacgccg
    10261 gccgcccgtg cgctgcgcgc atcatcgacg gtatcgccga cataccggag cctcgcgccg
    10321 ccggtgatgt ccgagatctt tagcaggccg tcgggggcag gtttgccatg gaccacgtcg
    10381 tctgtgccga cgacaggatc gaacggcagc gttctcgcga aacggtccaa tgtgacttgc
    10441 gcctcgtctc gcagcctgcc cgtaaagatg gccaggcggt aacgcgcacc aagccgctcg
    10501 agcaatccgg gcttcgcgat ccatcgttcc tggaagatca atccgtcctt tccatctcca
    10561 aaaaagaaag tctggaattg agtgaccacg gtatcgtaat ccacgttcac gccgtaatcg
    10621 cggatgatct tttgggagag cgcccagtcg ttgttccaac caccggcgtt cttccaatcc
    10681 tgaatgaact cgtggctgac ctcgcggccg gtgaagtact gcacggtacg gcgtatggtc
    10741 tccctgtacg acgccgtcac gtcgaccagc acgccgtcca tgtcaaagat gagcacttcg
    10801 tctggcatca ccagatctcc tcgagggccg ccaggaagcg gcgagtctgc tcgcgggtgc
    10861 cgacggtcac gcggacgcat ccgggaagtt cgtagctgcg gtcccggacg agcacaccca
    10921 tttcacgcag ccggtcgcga atttcgatcg cccggccgcc tgcccgaaag agtacaaaat
    10981 tcgccttgct atggaagtac gtgataccga gccgctccaa gccttcgtac agaagatccc
    11041 gggaggagat cgcctcggag acatagttcg aaatgtattc ggtgtcccgc acggcctctc
    11101 gcgcggccag cgccgccaac atgttcacgc tgtacggcga ctgggctttg tgaaggtagt
    11161 tgatattgac ctcctgcgag aagacgcaac ccagccgcat tgcagccata ccgtagacct
    11221 tcgagaacgt ccggctcacc aacaggttcg agaaacggcc gatcattccg agcgcggtga
    11281 ctccgcagaa ctcgtagtag gcttcgtcga tcagcacggc ggcgccgggg gctttttcga
    11341 ggatctgaac gactccatcg agcgagagcc ccgttccggt cgggttgttc gggttggcga
    11401 tcaggaccgc tcgggtagat ggccgtatcg catcgagcaa ttccgaaagc gggaacgcaa
    11461 ggtctgcggg gcggtagtcc acctcgcgga tcgacgctcc ggcaacttcc gcatagaacc
    11521 ggtacatggc gtaggacggc cgcaacaaca caacatcgtc gccattgtcc acgtacgtgt
    11581 tgacgatcac ctggatcgct tcgtccgtgc cgttcgtcaa gaggagttgg tcttcaacaa
    11641 ccccgaaaaa cccggctagg tccttcttcg cttcgacgta ctcggggtag acggctagtt
    11701 gatcctcggt cagcttctgt ttgaggaatt cgatcactcg gggtgaacag ccgagggtgt
    11761 tctcgttaaa gtcgaggcga agtttgccgg cgcggccacc cgtagggggc gagtacgggg
    11821 ccatccgcaa caccgcttca cgaggccgaa gaacaccggc gggaatttcg agccagtcag
    11881 ccacggatgc gcacctccac cgagcgcgcg tgagcctcga gtccctcggc ccgcgccaag
    11941 gtcgtaatag ccggagcaag cttgcacaga gaagtcgcgc ccagttcctg caccgagatc
    12001 accttcacga agtcggcgga agagagaccg gcacgcacac gggcggcccc ggaagtcggc
    12061 agcacgtggt tcggtccgga cgcgtagtcg cctgccgcct ccgggctgtg cggtccgacg
    12121 aagacgctgc cggcgcttgt gacgagcttc accagagagg cgtccgggat gctcaggtgt
    12181 tccggcgcga agcagttgga gatctccatc gcgcgttcga tcgacttcac gaggatgatc
    12241 atgctgttct tttcgatcgc cacgcgcgcc gtaggggcgg tcggcaggtc cgccagttgc
    12301 cggtccactt cacgtgccac cgccgtcgcc agccgctttg atgtggtgac gagaatcgct
    12361 gaggcgtctg tgtcatgttc cgcctgcgcc agcatgtcgg ctgcaatgac gcgcggatct
    12421 ccgtccgccg caacgatcag gatttcggtg ggaccggcga tgaagtcgat gccgacttct
    12481 ccggcaagaa gcttcttagc cgcagccacg tagatgttgc ccggtccaac gatgcggtcc
    12541 accttcggca ccgtctcggt accgaaggca aacgccgcta tagcgtgggc cccacccatc
    12601 cggtaaacgc tgcttacgtt gaggaaccgt gcggtgccga ggacttccgg cgacggccgt
    12661 ggcgaagcta cgcagcaacg ccgtacgccg gcgacctgcg ccgggatgac cgtcatcagc
    12721 agtgtcgagg gcaggggata acgtcccgag ggaacgtacg ctgccatggc gccgagaggc
    12781 cgcacgattt gtccggttcg atgaccgtcg cgcgagcgca ccgtctttgc caccggcagt
    12841 tgcatccgtg caaaatcgcg aatgttgcgg gcggcggtgc gaacagccct gcggaatgcg
    12901 gatgtgactc ccctgtaggc tgcctcggtg tcacttgccg gcacctccac ggacagccgt
    12961 ccacgctcca gtccgtcaaa ctttcgcacg tacttgagaa gcgcccgatc accgtgcttg
    13021 cgaacggcct cgaggatggg agcaacggtc tgctcggcct ctgtcagacg tgccgtccgg
    13081 cgttgaagga acctgtctgc ctgtttgtcg tctatgatcc gaatcattgg cgcctgcctg
    13141 ccgctgaccc acggctacat cacaatcttg ttcaacggat actccacgat gccctgcgcg
    13201 ccagcctcct tcagccgggg gatgatcgag cgcacggtcg attcgtcaag aatagtgttc
    13261 actgccagcc attcctcgtc gctcaagtgt gagattgtgg gccgcttgag tgcgggaagc
    13321 acggcaagca ccgcgtcgag gttctttcgc tcgacgttga gcatgaggcc gaccttgccc
    13381 agagcgttga ttgcgccctc caggagcatc cggatgtcct cgagtttccg gcgcttccac
    13441 tcgtcgcacc aggaatcgag attggcgatg agctgggtgt tggattcgag cacggtctcg
    13501 atgatcctca gcttgttggc ccggagcgag gagcctgtct ccgtaacttc gacaatcgcg
    13561 tcggcgagca ccggcggctt cacctccgtg gcgccccagc taaactcgac cttcgcggtc
    13621 actccatggc tcgcgagata gcggcgggtc gcgttgacga gttcggtcgc gatgaccttg
    13681 ccttccagat ccttcacgct gcgaaacgtc gaagcctcgg gcacggcgag aacccaccgc
    13741 acctttccga agctctgctt ggcgtagatc agatcggcga tggtccggac tttcgcctcc
    13801 gtctccatga cccagtcgtg gccggtgaga ccggcgtcga gcacgccgtc ttcgacgtaa
    13861 cgcgccatct cctgcgcccg aatgagcatg cactcgatct ccggatcgtc aatggccgga
    13921 aaatacgacc ggctgcttgt ggtgatgctg aagcccgcgc gccggaacag gtctatcgtg
    13981 gcgctctcga ggctgccttt ggggatgccg agtttcagtt tcactggccg cctccgtaga
    14041 cctgtttcgg gtcgtaaact ggcggctcgg attccatcca ctggccgttt tggagtgtcc
    14101 ggtagaagca gctcctgtag ccgttgtggc agactccggg tccaagggct tccaccttga
    14161 cgagcacggc atcctggtcg cagtcggtcg agatctcgcg gacgacgagc cggtgcccgg
    14221 acgtctctcc cttaagccac agcttgttcc gggaacggct gtagaaggtg gcaaagcccg
    14281 tttccacagt cttccggaac gcttcttcgt tcataaatcc gagcatgagg acgcgccctg
    14341 tagcatggtc ctgaatcacg gccggcgcca gcccgccgag cttgttgaag tctaggttca
    14401 taccggcact aacaaaaaag cccgcctgac ctccggcggg cttcctttcg ttttcgttcc
    14461 gaatttgctt aacagtcagc ccgtcgcgcg agttgagccc gatggtggtg atgatgccgg
    14521 gcgacggaca ggtttcgcat ggaaacctca gtataccaaa ccccagcggt cagaggcgcc
    14581 gatttcggtt ataatctcct cgtttcactg tggaggaggt ttatcgtgcg cagaatcatc
    14641 gcggcaggtc tcatctttct ctgttcaagc ttcttgtggg cgcagcagac tgcgcctccg
    14701 aagatccagg ttttgatcat caccggacag aacggacacg actggcgttc cgtcaccccg
    14761 gaactgcgga aagccctcga ggataccgga cgctttgagg tgcgtgtcac cgaggagttc
    14821 cgggatgcgg gtcctcaaac cctggctccg tacgacctcg tcgtgttgaa ttacttcgag
    14881 cgccgccagc ccgacctctg gtggggtgag agggctcaga acgcgctcct cgactacgtc
    14941 cgttcgggca agggcctggt gatgtaccat ttctccaccg cagcctttga tggctggaca
    15001 gagtacgaaa agatgtcggg cgggaactgg cgtcccaaca acgggcatca ttcgccatcc
    15061 cacgatttca ccgtggatat caaggactcc gaccacccga tcacccgcgg cctgaaaaag
    15121 tcattcccac agccacacga cgagttgtac gcgaacctga agtggcagcc ggcggatacg
    15181 taccatgtcc tggctaccgc ttgggacgac cattccctct acggcggcaa ggcgaggcag
    15241 cccacgcccg gcgccggatt ggaccagccg atgctgtgga ctctcaacta cggtaaaggc
    15301 cgtgtgttcg ggacagcgtt gggccatgac gccccggcgg tccggacgcc cgcgtttgtt
    15361 acaacattca ctcgcggcgc cgagtgggtc gccacgggcg cggtgacttt acccgtccca
    15421 cccgaaatgg ccgcccggtg atcgtcccgg gcgaaaatgg cccatcattt taccggaggg
    15481 ccggccggtt tcctggcgca ggcagcgacg aaacaggccg taagaggcca ggtcggaagc
    15541 gcgtcgagac gagagaagag tcctagcagc catcgaatcg gggtgtcggc gcgttttccc
    15601 agcgtaccgc gcagaccgag aaagagtagc gtcgaaatca gcctcgggtt gtggatcagg
    15661 cgggatttgg cggcgacttc cagcccgtgt ttctcgagag actgaatcaa gcccctcaaa
    15721 gaggtggtgt acccgagtgg aaacggcccg accccgagac gcgacaacag gcgaagcagt
    15781 tgatagagcg ggttgtacgc gttgtcgatg ctaatgacca gcactccgcc gggacgcagc
    15841 acgcgggcga gttcactcag cgcagcctga aactcctcgc gagtgtcgaa gtgatccagc
    15901 gtcgagttgg aaacgatcac gtcgaccaaa tccgagcgca acggtagact ccggacatcg
    15961 gcggccagaa cgaacgccgt gcccaatggc gaccgccgca aggcgcgcgc agcggtcggc
    16021 cagctgacat cgattccaat cacccggtgc ggcccgggaa acaaatcgaa aagaatctgg
    16081 tcctcgccga acgcctcctc gaacagatcc gttttcagga ccgactcaac cggacaaccc
    16141 tccgtccatc tccggatgag ctgctggtgg acctcccgct tctggcgagc caccagtggg
    16201 tcgagatacc aggagggcgc aggcgcttca gcgacgcgat cccatctgcc atcgttcgca
    16261 gacaccctcc cattgtacgc accggtcgcc tcgatctatt agagataaaa gagtcttata
    16321 attccattag cgtttgggag gtgtgaatga tcggaagctc cgcagcagtc ctccgagaaa
    16381 aactgaaccg gaggcaagcg ttgttggttc ccggttgcca cgatgcgctg agcgccaggg
    16441 tgatcgaaaa tgcgggtttt gaggcgatcc aggtgagcgg tttcgggctc gctggcagcc
    16501 tgctggcccg ccccgatgtc ggcctggtcg agatgaaaga tgttcttgag cacacctggc
    16561 acgtggtgcg tgccgtcggt atcccgatca tggccgacat cgacaccggc ggcggcaacg
    16621 cgatgaacgc cgccgacatc acggaacggc tgatcgcaat gggtatcgcc ggcgtcaaca
    16681 ttgaggacca ggtcttcccc aagcgttgcg gccacatgga aggcaagcag gtaatcggcg
    16741 ccgaggagat ggccggaaaa gtgcgagcga tggcggatgt tcgccgccgc gtgggcgctg
    16801 acatcgtgat caacgcccgg acggacgcct gtgcggtcta cggtctggag gaggcaattc
    16861 ggagagcgaa cctctatctc gaagccggag cggacatggc gtttctggac ggcatacgca
    16921 cacgcacgga cgtcgaacgc gccgtgcgag aagtcaaggg actgctctcg gtgaatctga
    16981 tggacgctgt taccggagtc aagaccgaac tcattccgat cccggaacta gcgcggatgg
    17041 gtgtcgctcg tgtaagcatc ccggtagcgt cgatcatggt ggtccacagg gcgttgaaag
    17101 agttcttcga tgcgttgcgt gcctccccga ccggcctact ggcgggcgag acacagtggc
    17161 tgtccagttt cgccgagtac accgatttcg tcggcctaaa cgaataccgg cggctcgaaa
    17221 atgagtacct gccgaaggcc acgctcgagg agaagtactc cgttcggtga ggaggcgatg
    17281 aacatgggca tgacgattgc ggaaaagatt cttgcccggg cctccgggcg agagaaggtt
    17341 gcgcccgacg aggtggtggt agctgccgtc gatctggcaa tgtcccacga gaacgccgat
    17401 ctcgtgcgga agagctttcg cgagatcggc gtcgcacgag tgtgggatcc aaagaaaatc
    17461 gtcattatcc tggatcaccg cgtgccggcc gagtccgaga agacagccac tacgcacaag
    17521 gcgatccggg agttcgtcaa ggagcaggaa atcgaaaatt tctacgacgt gggccgggga
    17581 ggtatctgcc accaggtgct ggcggaaaac ggacacgtca agccgggaat ggtggtggtc
    17641 gggacggact cgcacacgac gacgcacggc gcgtttgggg cgttcgcggc cggcatcggc
    17701 gccaccgaga tggcgggtgt ctgggtggag ggccgcctct ggttcaaagt cccggcgacg
    17761 atccgcatcg aggtgagtgg cgaactacgt tcctgggtct cggccaagga cctgattctc
    17821 tacgtcatcg ggaaactcgg cgcggatggc gccgactacc gagccgttga attcgacggg
    17881 ccggcgatcc gccgtctgac aatcgcctcg cggatggtgc tcgccaacct gtcgatggaa
    17941 atgggcgcga aggtggcgtt cacgccgatc gaccagatcg gacccgatgc ggacgccaga
    18001 tatgagcgcg tgctcacgat cgatgctgac tcaaaggtcg ccgaaccaca gattgcctgc
    18061 ccccacacgg tggacaacgt gcgtccgctg tccgaactgg gtgaagtgcc ggtcgatcag
    18121 gctgtgctcg gaagctgcac aaacggccgt ctggaggacc tggaaatggc ggctcggatt
    18181 ctcgccggcc gccgcgttca cgctcgtacg aggctgctcg tgatcccagc gtcgcagagg
    18241 atctacctag aggcgatgcg gctcggctac cttcagacaa tgatcgaggc cggcgcgatg
    18301 atcaaccctc cgggctgcgg gccgtgcgtc ggagttcatc agggcattct cgcggcggac
    18361 gaagtctgcg tatcaagcac gaaccgcaac tttgtggggc gcatgggaag caaggactct
    18421 caggtctatc tcgcctcgcc tgcggtcgtg gcggcgtccg cggtggcagg ccggatcatc
    18481 catccggcgg aaatcgtgga ggaggtgtgc gcatgagcga cgtctcgatg gttcttcgag
    18541 gcagggtagc ggcgaaactt ggcgacaatg tcgacactga cgtgatctac ccgggccgct
    18601 acctgaacat cacggaccga gagaagacgg tcgagcacct tttcgaactg acgtatccgg
    18661 agatccgctc tgggatccgt cccggcgatt tcctggtggg cggcaagaac ttcggctgtg
    18721 ggtcgagccg cgaacaggcg acggcggcag tgaagttcgc cggcgttgga gcggtgatcg
    18781 ccgtctcttt cgcgcggatc ttcttccgca attccatcaa cctgggtctc ccggtagtcg
    18841 tgtcgccggc ggcgctggaa attgaggccg gcgacgaact ggagatcgac ctcgcctccg
    18901 gttcgattcg aaacctcacg cacgggacgg cgatcacggc cgctccactc gatccgcggg
    18961 cggtcgaact tctcgaggcg ggtggtctca ttccgtacct gaagaagaag tacgcagcct
    19021 agggggcgga cgtcggttcg ccccggcatg tgacggagga ttcgtatgcc caagtaccga
    19081 attgcgtggc tcccgggcga cggaattggc cccgatgtgt gtgacgcggc tcgcctcgtt
    19141 ctggacgcgg tgggatttga ggctgagtat attcacggcg acatcggctg ggagttctgg
    19201 aagacagaag gtgatgcgct accgcagcga acggttgacc tgctccgctc caccgattgt
    19261 gcgttcttcg gcgccatcac ttccaagccg gttgccgaag ccgaacggga gctagcctca
    19321 gcgcttcgtg ggagcggcct gacttaccgc agcccgatcg tacggatgcg gcagttgctc
    19381 gacctctaca tctgcttgcg gccgtgcaag gcgtttcccg gcaaccctct caactatcgc
    19441 gaggacatcg atctcgtcgt cttccgcgag aacaccgaag gcatgtatat cggggtcgag
    19501 tttgatccgg tgcccgtgaa gttctacgac gaaccagcaa tgaagaagat accgcgcgac
    19561 gccgccatct ccgtccggag tatcacgcgg gacggctcgc ggcgtattgt tgaggcggcg
    19621 ttcgagcacg cggtgaagaa cggccgcagc aaagtgaccg cggtccacaa ggccaacgtg
    19681 cttcgaagta cttgcgggtt gttcctggac gtggcgaggg aggtcgccgc ccgctatcca
    19741 caagttgggt tcgaccatgc gaacatcgac gccatctgca tgtggctgct gaaaaacccg
    19801 cggaactacg aagtcattgt caccacaaat ctgttcggtg acatcctttc cgacctgtgt
    19861 gcacagcttg tcggcggcat gggcttcgcg tacagcggca atattggcag gaactatgcc
    19921 gtgttcgagc cgacgcacgg gtcggcgccg aagtatgccg gccagaacaa agtgaatccg
    19981 ctggccacaa tcctggccgc gaagatgatg ctggggtggc tcggcgagag gcagaaggca
    20041 atggagatcg agttggcggt tgcggacgtc gtcgagcgcg gcaacgtccg cacgtacgac
    20101 atgggcggct cagcgaccac cgaggacatg gcgcgtgcgg tcgtgcagac attgactttc
    20161 gggcaggcgg tagtggactc agagaggtga gccggcgatg gaaatgcggc ttattcgtga
    20221 gatcgctgac aagctccaga ttcccgatcc gtacttggag tactacggca agtacaccgc
    20281 aaagctccgc ctcgaactcc tcggggatgc cattcccccg cgccacggca aactgatcct
    20341 cgtcaccgcc atcacgccga ccagccatgg cgagggcaag acggtcgtct cgatcggcct
    20401 cgcacaggcc atcgagcggc tcggccgacg gagtcttgtc acgttgcgcg agccgtctct
    20461 cggcccggtc ttcggcctca aaggaggggc gaccggcggc ggcaaggccc aggtgctgcc
    20521 gagcgagatc atcaacctac acttcaacgg agatttccac gctgtcaccg ccgcccacaa
    20581 cctgctggcg gcgatgatcg actcacacat tcacaactcc aaccagcttc agatcgacgt
    20641 agacaacatc ttttggccgc gggcgatcga tatgaatgat cgcgctcttc gccacatcat
    20701 tgtgggactc ggcggcaagg tgaacggagt accgagagag accggcttcg tgatcaccgc
    20761 cgcgtcggag gtcatggcta ttctcgcgct cgccaactcg cgccaggact tgcggcggag
    20821 acttggcgag atcgtaatcg gattcgacct gaatggcaag gtattgcgtg ccggcgacct
    20881 gggagctacc ggagctctga tggtgctgtt gaacgaagcc atcatgccga accttgttca
    20941 aaccaccgaa cacacgccgg cgctcgttca tgcgggaccg ttcgccaaca tcgcgcacgg
    21001 caccagcagc gtcatttcac agaagatggc gttgaaactc gctgagtacg tcatcaacga
    21061 gaccggcttc ggcgccgatc tcggtgcgga gaagtacttt gacattgtaa tgcccgcctc
    21121 cggtctgaaa ccctcggcat gcgtgctcat cgcgactgtt cgcgcacttc aggcacaggg
    21181 cggcttcgac aacctcgctc gacacctcga aaacctcgcc aagttcgatc tgccggtagt
    21241 tgtcgcggtg aaccgcttct actcggacag cgccgcagac atcgccgcgg tgcgggagtt
    21301 ctgccgggcc tccggggttg agtgcgccga gaccgacgtc tactccgacg gtggcaaagg
    21361 cggcctggag ctggctgaaa aggtgatgac cgccgcggct gccggcgacc ccgcgcgtgt
    21421 tcgtccgctt tacgtgccgg atcttggcat cgagcaaaaa gttgagacga tcgcccgggg
    21481 aatctacgga gcggacgggg tgtccttcga gctctccgcg cggaagaaaa tccgaaagtt
    21541 tgccgaactc ggattcagtc gtctgccggt ctgcgtggcc aagactcagt cgtccctctc
    21601 agacaacccg aggctgctcg gacggcccac gggctggacc cttatgatca ccgacgccca
    21661 cctcgccgcc ggcgccggat tcatcgttgt tgtggccggc aacatgctgc tgatgcccgg
    21721 cctggggaag gatccgcagg ccgcaaggat ggatgtggac gaggatgggc gcattctcgg
    21781 tctcgtgtga ggttgccgcc aacccccgaa tccggttgaa tagacggaag tccatctcac
    21841 cggagactga aaaccatgat ccgcagaagc tttctggccg gcctcgccgg cgcactacca
    21901 gtggccgtag cggccgccac tgaacaatcg aaccggccga ggttcaaact aaggtacggt
    21961 ccccaccccg ggatgtttaa gaactccgcc ggggaggaca tcattgacca gatcaagttc
    22021 gccgccgatc agggcttcac cggctgggaa gacaacggtg ccatcagccg gccgccggaa
    22081 ctgcaattcc ggatcgggag cgcgctgcaa cagctcggaa tgaccatggg cgtttttgtg
    22141 tcgcttgccg atttccgcac ctccgacttc gtcaccaaga ccgacaagcc gtaccaggac
    22201 agcctcaggg acatggcaaa gcgggcggtg gagacggcga agcgcgtcgg aaccaagtgg
    22261 actaccgtcg tgccgagcac tgtgaacaac tcgctcgatc ccggtttcca gacggccaac
    22321 tgcatcacca acctcaaggt gatggcggag gtgtgcgagc cagccggcct catcatggtg
    22381 cttgagccac tgaactggta cgcgaaccat ccgggcttgt tcttgcggtc cgtgccgcag
    22441 ggctatgcca tctgcaaggc cgtcgactcg ccaagctgca aaatccttga tgacctttat
    22501 catcagcagg tggacgtcgg gaacctcatc ccgaacatgg agaaagcttg ggacgagatt
    22561 gcgtacatcc aagtgggcga caacccgggg cgcaaggagc caacgaccgg cgagataaac
    22621 taccgcaaca ttttccgctg gctgcacgcg cgtcgctatg acggcgtggt cgggatggag
    22681 cacggtctca gcaagccagg caaggagggc gagatggcgt tgatcgcggc ttaccgggag
    22741 tgcgacagct actagtcacg gcgtctggac cgctgctgaa cgggagaggg tttggaggcg
    22801 gctgtaagtc agggtgcgcc agatccactc cgttggcccg aacgtgaaac gggcggtcca
    22861 ccaggtgctc agagggacct gtgccgagta gatcagtatc gtcgggatca ggcctgcgag
    22921 tggcccgacc ttgccgaaaa gtccgaagcc ccaactgttg tacagggtcg tgcaaaccac
    22981 ggactgcagc aggtagttgg agagcgcggt ccgtccgacg gcgccgaacg gccggaggac
    23041 gcgccgccac gtcccttcct gagagagcag gaggccactg gatgcataaa agaggctgag
    23101 tgccggcatg gcgatgctgc cggcgatgcc gacgaggaat ccctcacggg tgaactcggt
    23161 tggattgggc cgccagatct ccaacatcgc aaccacggcg gcgttcccta cgagtcccac
    23221 cacgagtccc cagttgcggc agcgtcgaag gaccggttcg tactgtgccg ggctgcggat
    23281 gatgcccgta cgccacaccc agaggccaaa caggaatagt ccaagaacgc gggggtagaa
    23341 gaagacgagg ccgattccat tgaaggccat gtctttcact cgcacccgca gcatctcggc
    23401 gtacgttccg ttcgcaaaga tctgaatgat tcgctgcagc tcctcgggag tggggcgagg
    23461 tggtgtcggt atcaccgcgc ccgccgagtg cgctgccatc gccgcgccca tcagcatcat
    23521 cggccaggca taaaagatca ctgcccagat gaggattgtg ctttgagagc gtttccggaa
    23581 caagagaagg aagatgccca tcaaagcgta gggcgcgagg atgtcacccc gccagaggag
    23641 gaacccgtgc gcaagccaga taatcaggag cacggtcagc cggcggacgt agaacagtcg
    23701 gctcgcgaca ccgcgagcgt ccgcacgctc catctgaacg gcgaaaccga tgccgaacag
    23761 gagcgaggac agggtgacaa acttcccggt aacgaacagg tcgatgaagc cctgagcaat
    23821 ccggtctccg ataccttgcc acgtgagcga gtgatcgaat taggtggcct gcggcgaatt
    23881 gaaagcccgc atgttcgcgg tgatgatccc gaacagggcc aggccccgaa gcacgtcgag
    23941 gatttcgatg cgctcccgcg aggtgatcgg tgtcatcgag ctttcagtgg ttttcccaga
    24001 ggacacgctc tcccccttgt gaaagtaaag caattgtagc aaggcgggga agcgtgccgg
    24061 ttccgtactt aggataaatc gcagaagtct tcaggacagc gcttggtcga gatcttcgag
    24121 gatatcctcg atggcctcga tacccaccga aaggcgcacc aggttgtcac ggatgcccat
    24181 ccgctcgcgt tgcttcggcg acacttcgcg atgagaactc atcgacggat agagaaccat
    24241 cgtgtgcaca tcacccagcg acgtggccgg tacgatcatc ttcaggcgct ccatgaaggc
    24301 gaacacttcg gcgcgcgccg cgtcccgcat ctcgaaactg accatcgctc cgtaaagccc
    24361 ttccgggaat agacgccgga tcgttgccgc gtcagggtgg cccggatcgc ccggaaaatg
    24421 gacgcgcgcc acgcacgggt gatctgccag ccgcgcagcc acgcgacagg cgttcgcgca
    24481 ctggcgctcc atccgaaggg gtagcgtctt caggcc
//
LOCUS       DATIFQ010000234         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_841285, whole genome shotgun sequence.
ACCESSION   DATIFQ010000234 DATIFQ010000000
VERSION     DATIFQ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841285"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VLE22_10915"
     CDS             <1..1166
                     /locus_tag="VLE22_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14961.1"
                     /translation="YIASTNNRFYTAVEQTYGAVPAIDAGHRFPAVKLSIRQAPDGVQ
                     RKDKTGSRTFFGLPPHTRNQTTFELRTYMTGWGDQSREPAYGPMFQAALGGAPLFFNG
                     ALAGAGAAGRILPFSQPHGLAPGQAVTFGGEIRFVTAIADDFGVQLNAPFTMLPVEGS
                     PAGATVTYSPATKLKSTSIFDYWSPGEAVQRILCGAGVDEMGVRVNGDFHEFTFSGAA
                     ADVLDSSSFALGQGQLESFPPEPAPGIFDYTIIPGHLGQAWLGTAPDRFFTITEAEIS
                     LDNDLDLRAREFGANGPRCIAAGMRNVQVDFSLYEKDNEATKALYQAARQRSPIEIMF
                     QLGQEAGQLFGVYLKSVVPEVPEFDDSEKRLAWRFASCRAQGTMDDEIYVAFG"
     gene            1252..1617
                     /locus_tag="VLE22_10920"
     CDS             1252..1617
                     /locus_tag="VLE22_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14962.1"
                     /translation="MGYTSCVTIESQCRPGVSLTIRRMSFGRRIELTRQIRELARKIE
                     FLEAGSDTGEKIESALLKAEVERLYLTWGLVKVEGLEVDGATATPESLLNEGPEDLCR
                     EAITAIKAECGLTDAERKN"
     gene            1692..1946
                     /locus_tag="VLE22_10925"
     CDS             1692..1946
                     /locus_tag="VLE22_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14963.1"
                     /translation="MKRRCGFLPAALETRERAVWAKGGAAATTCPKSLVTPQSLEWVE
                     RYYAWKLSGQGDLTGVDARTADAFCVLENEMRAEGIHAGQ"
     gene            1933..2502
                     /locus_tag="VLE22_10930"
     CDS             1933..2502
                     /locus_tag="VLE22_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14964.1"
                     /translation="MPASDLDGTVRELWNFAGADVSGQLERLVQAGRAQLEALGENTL
                     AVSQNTKAQTSGSGKSAAGEIARTASGLLGSGFGLSPLLTGLIGLFRGSEQTLAATAP
                     LATYTQPPAIRFDGALSRQSGQPVYRADYEQGGLPRAPQATTSLPPITVQVQAMDSRS
                     FLDHSDEIARAVREAMLNAHALNDVVNEW"
     gene            2509..2868
                     /locus_tag="VLE22_10935"
     CDS             2509..2868
                     /locus_tag="VLE22_10935"
                     /inference="COORDINATES: protein motif:HMM:NF020901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HSB14965.1"
                     /translation="MADFPILKTGAVAQYPAERTTTFSTRVLRFVDGTEQRYRQHSSP
                     LRRWVIRLNLLDEEELANLEAFFQSEQGRAGTFAFTDPIDGTTFPSCSLESDRIELEY
                     KDVFRGEAVMVIRENRD"
     gene            2870..>3128
                     /locus_tag="VLE22_10940"
     CDS             2870..>3128
                     /locus_tag="VLE22_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14966.1"
                     /translation="MLYFPQLSTGALTQYPLSKMREARTVINLFQDGRALRLLDGAHE
                     LVEWRISLTGLSEAERNAIESLFQSTEGRLGEFTFLDPTDNL"
BASE COUNT          633 a          867 c         1108 g          520 t
ORIGIN      
        1 gttacatagc atccactaat aaccgcttct acacggcggt ggaacagact tacggcgctg
       61 tgccggcgat cgatgccggg cacaggtttc cggcggtcaa gctgtcgatc cggcaggcgc
      121 cggacggggt gcagcgcaag gacaaaacag gcagccggac gttctttggt ctcccaccgc
      181 atacacgaaa ccagacgacg ttcgagctgc ggacgtacat gaccggctgg ggtgaccaga
      241 gtagggaacc ggcgtacggg ccgatgtttc aggcggcgct cggcggtgcg ccactgttct
      301 tcaacggtgc gcttgccggc gcgggggcag caggcaggat ccttccgttc tcgcaaccgc
      361 acggcctcgc acccgggcag gcggtgacat tcggcggcga gatccgattc gtcacggcaa
      421 ttgccgacga tttcggcgtg caactgaatg cgccgttcac gatgctgccg gtcgaagggt
      481 cgccggccgg ggcaacggtg acttactcac cggcaacgaa gctgaagagc accagcattt
      541 tcgattactg gagcccgggc gaggcggtgc agagaatcct ttgcggggcc ggggttgacg
      601 agatgggggt ccgggtgaat ggggacttcc atgagttcac attcagcggc gcggcggcgg
      661 atgtgctcga cagcagcagc ttcgcgctcg ggcaagggca actggagagc ttcccgccgg
      721 agccggcacc ggggatcttc gactatacaa tcattcccgg acatctcggg caggcgtggc
      781 ttggtaccgc tcccgaccga ttcttcacca tcacagaagc cgagatctcc ctggacaacg
      841 acctggacct gcgggcgagg gagttcggcg ccaatgggcc acggtgtatc gcggccggga
      901 tgcgaaacgt acaggtggat ttcagcctct acgaaaagga caacgaggcc acgaaggcgc
      961 tgtaccaggc ggcgcggcaa cgatcgccga tcgagatcat gttccagttg gggcaggagg
     1021 ccgggcagtt attcggcgtg tacctgaaga gcgtcgtgcc ggaggtgccg gagttcgacg
     1081 acagcgagaa gaggctggcg tggaggttcg cgagctgccg ggcgcagggg acgatggacg
     1141 acgaaatcta cgtggcgttc gggtgaggct gaaaacccgg gacagacacc aatttcgcaa
     1201 aggcggctaa atttggagtc agtcccgggt tttcgctgcg gagggtgagg tatggggtac
     1261 acaagctgcg tgacgattga gtcgcagtgc cggccggggg tgtcgctgac gatccgcagg
     1321 atgtcgttcg gcagaaggat cgagctgacg cggcagattc gggaactcgc gcggaagatc
     1381 gagtttctgg aggcgggcag cgacacgggg gaaaagatcg aatccgcgct gctcaaggcc
     1441 gaggttgagc ggttgtatct gacgtggggc ctcgtgaagg tggagggact ggaggtggac
     1501 ggggcaacag ccactcccga gtccctcctc aacgaggggc cggaggacct ctgccgggaa
     1561 gccatcacag ccatcaaagc cgaatgcggg ctaacggacg ccgaacgaaa aaactgatcg
     1621 tcgccttcca tttttgttcc caggaccagg ccggctggag gtgcgacgca tgcagggcga
     1681 agggtctgga gatgaagcgg cggtgcgggt ttctgccggc ggcgcttgag acgagggagc
     1741 gggcggtttg ggcgaaaggc ggggcggcag cgacgacgtg cccgaaatcg ctggtgacgc
     1801 cgcagagcct ggaatgggtc gagcgctatt acgcatggaa gctgtctggg cagggggacc
     1861 tcacgggggt ggatgcgcgg acggcggacg cgttttgcgt gctggagaac gaaatgagag
     1921 cggagggaat tcatgccggc cagtgatctc gatggaactg ttcgggagtt gtggaacttc
     1981 gcaggggcgg atgtctccgg ccagcttgaa cgactcgtgc aagcgggcag ggcacagttg
     2041 gaggcgctcg gcgagaacac gctggccgtc agccagaaca cgaaggcgca gacctcggga
     2101 agcgggaaat ccgcggccgg cgaaatcgca cgaacggcgt cgggacttct cggcagcggg
     2161 tttggcctct cgccgctgct gacggggctg atcggcctct ttcgcggcag cgagcagacg
     2221 ctggcggcaa cggcgccgct cgcgacatac acacaacctc cggcaatccg gttcgatggc
     2281 gcgctgtcgc gacagagtgg ccagccagtg taccgggcag actacgagca gggtggactc
     2341 ccccgagcgc cgcaggcaac gacgtcacta cctccgatca cggtgcaggt gcaggcgatg
     2401 gacagccgct cgtttctcga tcacagcgac gagatcgcaa gggcggtgcg agaggcgatg
     2461 ctgaacgccc acgcattgaa cgatgtcgtg aacgagtggt aggccaccat ggcggacttt
     2521 cccattctga aaacgggcgc ggtcgcacag tacccggcgg agcggacgac cacgttctcc
     2581 actcgcgtgt tgcgattcgt cgacggcaca gagcagcggt acaggcagca ctcctcaccg
     2641 ttgcggcggt gggtgatccg gctgaacctg ctcgacgagg aggaactcgc caatctcgaa
     2701 gcgttcttcc agagcgaaca gggacgggcc ggaacgttcg ctttcacgga cccaatcgac
     2761 ggcacgacgt ttccgagttg cagcctggag agcgaccgga tcgagcttga atacaaggac
     2821 gtgttccggg gcgaggcggt aatggtaatc cgggagaacc gagactgaaa tgctctactt
     2881 tccgcagctc tcaaccggcg cgttgacgca ataccccctg tcgaaaatga gggaagcccg
     2941 gacggtaatc aacctgtttc aggacggcag ggccttgcgt ctgctcgatg gggcgcacga
     3001 actcgttgag tggcggataa gcctgaccgg cttgagtgag gcagagcgaa acgccatcga
     3061 gtccctgttt cagtcgacgg aaggacgcct tggagagttt acttttctcg atcctacgga
     3121 caacctgc
//
LOCUS       DATIFQ010000235         8998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1798887, whole genome shotgun sequence.
ACCESSION   DATIFQ010000235 DATIFQ010000000
VERSION     DATIFQ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8998
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798887"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VLE22_10945"
     CDS             complement(<1..333)
                     /locus_tag="VLE22_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSB14967.1"
                     /translation="MVPESALQQFRTSLRGQLFTPGEPGYEEARKIHNALIDRRPAVI
                     ARCAGVADVISAVNFGRGQSVVVSVRGAGHNVAGTSLCDGGMVIDLSAMKGIHVNPVA
                     RTARVEPGV"
     gene            complement(417..683)
                     /locus_tag="VLE22_10950"
     CDS             complement(417..683)
                     /locus_tag="VLE22_10950"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HSB14968.1"
                     /translation="MCRSIKTLFNFDPPATDEEIRAASLQFVRKISGFSKPSKANEDS
                     FRAAVDEIAGIASRLLVSLETNTVPKNREVEAAKAKARAGRRLR"
     gene            757..1881
                     /locus_tag="VLE22_10955"
     CDS             757..1881
                     /locus_tag="VLE22_10955"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB14969.1"
                     /translation="MRSHERTRGLLRAPKLAWDVLVRGRYDFTYDQMPMTASRMAWAK
                     RFNLLKAGANLLHRRLRPWSMPLHMQFELTNYCSLRCPVCPTGSKGVNRRYQAIDMGL
                     FDRVMDEVGPHLLTASLWAWGEPLLHPRLKEILRHARKHDVITLLSTNGQCLNQDRIL
                     GALVDEPPDYLIVAFDGLTDGTNSQYRVGATLAPILDGVRKLAELKRLRGLSTPLLNM
                     RYIVMNHNQHEVPQLEEFARRHDFELLTIRSIFFIESTTDTQASRRLAPGEEFWKTCG
                     HVKGGNVRRGDFICQEPFWFPTIFADGTVVLCEQDYNAELALGRVEGDVSFTRLWRGS
                     RAAQLRGIIRDGMDSVSFCRKCPYIDRPITDFNVDARVLT"
     gene            1896..2969
                     /locus_tag="VLE22_10960"
     CDS             1896..2969
                     /locus_tag="VLE22_10960"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14970.1"
                     /translation="MANPGKTIRLGIIGCGRVAEERHLPPLLRLPDVRVVATVDLDAA
                     RSRRLADRLDADLRFTDYRAVLERPDVDAVAVLTPTQSHAEIGLAALDAGKHLLVEKP
                     LAMNLAECDRLIARAAQSSCKVVVGFNLRWHRLVRRTREFLSTGALGRIKAIRSAYTH
                     DRSGETAPDWHRKLELGGGVSFNEAAHHFDLWRYFTGGEVEQVFSASVPSAYYEDETN
                     VVTARMSDGILASGVFTFKTGPNSEVEIYGENGRLCLSLYRFDGMEFFPCAIYPGNLS
                     DRLRKTVETLGRLPEAVPVLRQGGDFQATFDGLWRHFADCILHDLPSHCTLEDGRSSL
                     RIALAAIHSARSGAPVQVRTVGV"
     gene            2979..3893
                     /locus_tag="VLE22_10965"
     CDS             2979..3893
                     /locus_tag="VLE22_10965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB14971.1"
                     /translation="MARSPETIDFSIIVPTRDRHAQLAACLDALAELRYPASRFEVVV
                     VDDGSRTPVEPVLEPRRERLQIRLRTQPGAGPARARNHGADAARGRFLAFTDDDCAPA
                     PDWLEKLAVRLADAPDAVAGGCTVNLLKDNLFATASQSLLSYLYEYFSDNRGVPWFLA
                     SCNFALAASTFHSVGGFDPSYPTAAAEDRDICDRLRLHGYSMVYAPDAVVYHEHALDL
                     RKFWSQHFGYGRGACRFWRAHRDRSGGKIRIEPPAFYWGMLMYPLHHRSRAPLAVAAL
                     LLVSQVANLAGFLCEWRLCGVRLNSAIR"
     gene            4373..5629
                     /locus_tag="VLE22_10970"
     CDS             4373..5629
                     /locus_tag="VLE22_10970"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HSB14972.1"
                     /translation="MNILVLNAGSSSLKARMYRVGGEQLPEDPPEPLWDAFVDLSLQP
                     GVAHIRVRSAGAAIQKESPCTHLPDTLDGVLESLWDGPTAVLKDRSEIHIAGHRVVHG
                     GQELRESTRITPSVKAVIERLGPFAPAHNPVELSVIEAAERILGSDVPQLAAFDTAFH
                     AKLPPEVYTYPGPYEWLDHGIRRYGFHGISYQYTTRRAAHILGRDLSGLRLIACHLGN
                     GCSLAAVRNGVGADTTMGFTPLEGLMMGTRPGSLDPGILIYLVRHRGYDAAELNRILN
                     EECGLKGVSGISRDMRQILAERDKGNPRAALAFDIFIHKLRCHISAMLPNLGGLDALV
                     FTAGIGENSPEVRAAVCEAFGFLGMKLDHGKNVNSPADTDIATPDSAVRVLVVQTRED
                     WEIARECFRLCSHATPRESCEENPAN"
     gene            complement(5576..7555)
                     /locus_tag="VLE22_10975"
     CDS             complement(5576..7555)
                     /locus_tag="VLE22_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14973.1"
                     /translation="MSRKKLILAALLLFLLNVWISGRLFRFEFVDHMNSIEGTHIALG
                     RWVSENWRDLSWFPLWYGGIPYQNTYPPLHHWIVGLVSSVGDVPPARAYHAVTAVFYS
                     LGPVTLFLFAAWLSRSVGIGFLAGLVYSVFSPSTLLIPIVRYDAGGVWGARRLQTLVQ
                     YGEGPHIASMTLLPLVLLLVIVAVERRHPAWSFAASLGLASVALTNYLGAASLALAVV
                     AWLFASDEIRLGTWLRAAMLGLYAYALACSWLPPSTLLAISRNERYLSGTLPPSRWLY
                     LALMVLGAAALLWLLRRVNAPPQLRFAALFLTPLAAFTLASEWWKVSLMPQGHRYHLE
                     MEMALVLTGVFGARYLLERSSRRLRVAAACLLVVLCVYPAIRYRKCGKRLIRPVDITQ
                     RIEYREARWAERNMAGRRVMLPGSVGFFLNVFTDTPQFAGGFDQGIVNPNYAAFHYQL
                     LTGENAGNQEGTVAVTTLKAFGVDAVGVSGPRSREVFKPFRNPRKFDGLLRELWRDAD
                     DVIYDVGRRSKSLAHVIRRADLPARAPIHGLDLDPMRPYVAALDDPALPAAEMKWSSR
                     HSAVVSASIEPGQVLSVQISYHPGWSATVAGAPVRLYGDALGQIVVEPGCEGPCTVEL
                     VYDGGMEMRVARIVSWSALIGGILFAAFARRRMRA"
     gene            complement(7552..8868)
                     /locus_tag="VLE22_10980"
     CDS             complement(7552..8868)
                     /locus_tag="VLE22_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HSB14974.1"
                     /translation="MSTLLGRLERYFEFQTLGANWKTEILAGVTTFITMAYIIFVNPS
                     ILHEAGMPFAAVVAATCLSSALGSLLMGAYARYPIALAPGMGLNAYFAYAVVKGMGLP
                     WQAALGAVFLSGVAFAVLTLCGVIRRVVASIPTELYSAIAAGIGFFIALVGFRNSGII
                     VASKETTVALGNLGEPRTALALFGLLLIAALLAWRVRAAMLIGILATTAAGAAAGLVH
                     WQPQMYRLSDVSATAFRLDLSSAFGLGFIEIVFVFFFVDVFDNVGTLVAVGKKAGLFN
                     EAHEIPRINRILLSDAVASTAGSLAGTSTVVSYIESAAGVVAGGRTGVTSVVTGLLFV
                     VALFIAPMVGVIPAAATAPALIIVGSLMVSHISEIDWSNPMIAVPAFLTMTAIPLTFS
                     IANGLAFGFTAYTLLKVLRGEFRTVSWLVYILTALFIARFLYLGAG"
BASE COUNT         1761 a         2813 c         2827 g         1597 t
ORIGIN      
        1 gacgcccggc tccacgcgag ctgtgcgcgc cacggggttc acgtgaatcc ctttcatcgc
       61 ggaaaggtcg atcaccatgc cgccgtcgca caggctggtg cccgctacat tgtggcctgc
      121 tcctcgaact gaaaccacga cgctctggcc ccgtccgaag ttgaccgccg aaatcacgtc
      181 ggccacgccg gcacaccgtg cgatcactgc cgggcgccgg tcaatcaacg cattgtggat
      241 cttgcgggcc tcctcatagc ctggctcgcc gggagtaaac agttggcccc gcagcgacgt
      301 gcggaactgt tgcagagcag attccggaac cataagccca gcccccttgt tcaataggct
      361 actggcgaac cgcgtttaaa gtcaaagcgc agcgcccgaa gaccgcccgt cacggctcag
      421 cgcaatcgcc gcccggcgcg agctttcgcc tttgcggcct cgacctctcg gttcttcggc
      481 actgtgttgg tttcgagcga aacgagaaga cgacttgcga tgccggctat ttcatccacg
      541 gcggcccgga acgaatcttc gtttgccttc gacggcttgc tgaatccgct gatctttctc
      601 acgaactgga gcgaagcggc tcgaatctcc tcgtcagtgg ccggtgggtc gaagttgaag
      661 agggttttaa tactcctgca cattccgtat gacctcaagg tatcagatcg cttaagggct
      721 gcttcgggat gcgataatgg acctcccgac gacggcatga gatcgcatga acgcactagg
      781 ggtctgctgc gggctcccaa gctggcttgg gatgtgctcg tccggggacg ctacgacttt
      841 acatacgatc aaatgcccat gaccgccagc cggatggcct gggctaaacg gttcaacctc
      901 ttaaaggcag gcgctaatct tctccaccgg cggttacggc cttggagcat gccactgcat
      961 atgcagttcg agctgaccaa ctactgcagc ttgcggtgtc cggtgtgccc gacgggctcc
     1021 aagggggtga accgtaggta tcaggccatc gacatggggt tgttcgatcg cgtgatggac
     1081 gaagtgggtc cacacctttt gactgcgtcg ttgtgggcct ggggagagcc tttgctccac
     1141 ccgcgcctca aggaaatcct ccgccacgct cgcaagcacg acgtcatcac tctcctatcg
     1201 accaacgggc agtgcttgaa ccaggaccgc attctggggg ctttggtcga tgagccgccc
     1261 gactacctca tcgtggcgtt cgacggactg acggatggga cgaactctca ataccgcgtg
     1321 ggcgcgacgc tcgcgcccat cctggatggc gtcaggaaac ttgccgaact gaaacgcctc
     1381 aggggcctta gcaccccttt gttgaacatg cgctacatcg tgatgaatca caaccagcac
     1441 gaggttccgc aactggagga atttgcccgc cggcatgatt tcgaactgct gacaatccgc
     1501 agcatcttct tcattgaatc gactaccgac acgcaggcca gcagacgatt ggcgcccggc
     1561 gaggagtttt ggaaaacctg cgggcatgtc aaaggtggca atgtccggcg cggggacttt
     1621 atctgtcagg agccgttctg gttcccaacg atttttgcgg atggcaccgt cgtcctctgc
     1681 gagcaggact acaatgcgga acttgccctg ggccgcgtcg aaggcgacgt ctccttcacc
     1741 cgcctttggc gcggcagccg agcggcacag ctacgcggga tcatccggga tgggatggac
     1801 agcgtgagct tctgccggaa gtgcccttac atcgatcgtc ccatcaccga tttcaacgtg
     1861 gatgcgcgtg ttctgacgta gaggagaact cttctgtggc gaatccggga aagacgatac
     1921 ggctcggaat catcggatgc gggcgggtag cggaagaacg tcacctccct ccgttgttgc
     1981 ggttgccgga cgttcgcgtt gtggcgaccg tggacctgga tgcagcgcgg agccgccgcc
     2041 tggcggaccg cctcgacgcc gatcttagat tcaccgacta tcgcgccgtg ctggagcgcc
     2101 cggacgttga cgcggtcgcc gtcctcacgc ccacgcagtc gcacgcggag attgggcttg
     2161 ccgccctgga tgccggtaaa cacttgctcg tcgagaagcc gctcgccatg aatctggccg
     2221 agtgcgaccg gttgatcgct cgcgccgcgc aatcatcctg caaggtggtg gttggcttca
     2281 atctacgctg gcaccgcctg gtccggcgca cccgcgaatt tctgagcacc ggggcccttg
     2341 gccgtatcaa agcgatccgg agcgcctaca cgcacgatcg ttccggtgag accgcgccgg
     2401 actggcaccg caagctggaa ctgggcggcg gtgtgtcgtt caatgaagcc gcgcatcact
     2461 tcgacctgtg gcgatatttc acgggaggcg aggtggagca agtcttctcc gccagcgttc
     2521 cgtccgcgta ttacgaggat gagaccaacg tagtcacggc gcgcatgtcc gacggcatcc
     2581 tggcgagcgg cgtcttcacg ttcaagaccg gccccaacag tgaagtggag atttacggcg
     2641 aaaacggccg gttgtgcctc tcactctacc ggtttgacgg catggagttc ttcccgtgtg
     2701 ccatctatcc cggcaacctg agtgacaggc tgaggaagac cgtggaaacc ctgggcaggc
     2761 tcccagaggc ggtcccggtt ctgcgccagg ggggagattt tcaggcaacc ttcgacggct
     2821 tgtggcgtca cttcgccgac tgcatcctgc acgatctgcc atcccactgc accttggagg
     2881 atgggcgcag cagtctgcgg attgctctcg cggcgatcca ttccgctcgc tccggcgcgc
     2941 ccgttcaggt gcggacggtg ggagtctagg gcgcaacggt ggcgcgctcc ccagagacca
     3001 ttgacttctc aatcatcgtt cccacccggg accggcatgc ccaactggcg gcctgccttg
     3061 atgcgctcgc ggaacttcgg tatccggcct cgcgcttcga ggtcgtggtc gtagacgacg
     3121 gcagccggac gcctgtggaa cctgtcctgg agccgcgccg cgagcgcctt cagatcaggc
     3181 tccgaactca acccggcgcc ggccctgcgc gcgcccgcaa ccatggcgcc gatgcggccc
     3241 gggggcgctt cctcgccttc acagacgatg actgtgcccc cgccccggac tggctggaga
     3301 agctcgcggt ccgtctcgct gacgctccag atgcggtcgc cggtggatgt accgtgaatc
     3361 tgctgaagga caatcttttc gccaccgcga gccaatcgct ccttagctat ctctacgagt
     3421 acttcagcga caatcgcggc gtaccgtggt tcctggcctc ctgtaacttc gcgctcgccg
     3481 caagtacttt ccactccgtg ggcggcttcg acccatccta tccgacggct gccgccgagg
     3541 acagggacat ctgcgatcgt ctccggctcc acggctatag catggtatac gccccggacg
     3601 ccgtggtcta tcatgagcat gcgttggacc tgcggaagtt ctggtctcag catttcggtt
     3661 acgggcgagg cgcttgccgg ttttggcgcg cgcaccggga ccgctcaggc gggaagattc
     3721 gcatcgagcc gcccgctttt tactggggca tgctgatgta tccactgcac caccgtagcc
     3781 gggcgcccct ggccgtggct gcgcttctcc tcgtaagtca ggtcgcgaat cttgccggat
     3841 tcctgtgcga gtggaggctt tgcggagtgc ggctgaactc cgcaatccgt tgattctacg
     3901 agaggtttcg tgtcgacttc gtgccacaat gggacgaaat cattgaatac ctgttattct
     3961 atatagtaga tgcaaaaaca ccatgccaat ctctcttcgt accaattcaa acgggcaaaa
     4021 acccgtgggc tcatcgaggt gctcaatgag ggttcgtacg tcatggctca gtacagtgag
     4081 cgcacgggca cggtgaaatg gcaacgcgtc gtgctggcct cgcagaggga attgattgag
     4141 aagtggctgg gcgagcacta tccggtccaa gctgaaactc aacctgagtc gagagctcgg
     4201 gtctcggccc agtcatcccc ttcatccgtc cgaaaacctt ccaggaagaa accggccaag
     4261 agaaccgcgg cctagaaaat acagcgtgct gtatcctgag gtgatgcgga ggagggccgc
     4321 tcattgcggt ctccgatcgg actgcagttc agtgtagccg cgcccacttc agatgaacat
     4381 tctcgtgctt aacgccgggt cgagcagcct gaaggctcgg atgtatcgtg tcggcggcga
     4441 gcaacttccc gaggatcctc ccgagccgct ttgggacgcc tttgtcgatt tgtccttgca
     4501 gcctggagtg gcacacattc gtgtgcgcag cgcgggagcg gcgattcaga aggagtctcc
     4561 ctgcacgcac cttcccgata cgcttgacgg cgtcctggag tccttgtggg acggaccgac
     4621 ggccgttttg aaggaccgtt ccgagattca catcgccggt catcgcgtcg ttcacggagg
     4681 ccaggaactc cgcgagagca cgcggatcac gccttccgtc aaggctgtga tcgagcgact
     4741 ggggccgttc gctcccgcgc ataacccggt cgaattgagc gtcatcgagg cagcggagcg
     4801 gattctggga tcggacgttc cccaactggc cgcgttcgac actgcgttcc acgccaagct
     4861 tccccctgag gtctacacct atccgggacc gtacgaatgg ctggaccacg gcatccgccg
     4921 ctacggcttc cacggcatca gctaccagta cacgacgcgc cgggctgccc acatccttgg
     4981 tcgggatctc tccggtctcc ggctcatcgc gtgccacctc ggcaacggct gctcgctcgc
     5041 cgccgtgcgc aacggcgttg gcgccgacac cacgatgggc ttcacaccgc tcgaagggct
     5101 gatgatgggc acacgtccgg gatcgctcga cccggggatt ctgatttacc tggtacggca
     5161 ccgcgggtac gatgccgccg agctcaaccg gattctgaac gaggagtgcg ggctgaaagg
     5221 cgtctccgga atctcacgcg acatgcggca gatcctggcc gagagggaca agggcaaccc
     5281 gcgcgccgcc ctggctttcg acatcttcat tcacaagctg cgctgtcaca tcagcgccat
     5341 gcttccgaac ctcggcgggc tcgatgcgct cgtgttcacg gccggcatcg gcgagaactc
     5401 gccggaagtc cgcgcggcgg tgtgcgaagc tttcgggttt ctcgggatga agctcgacca
     5461 cggcaagaat gtgaattccc cggcagacac cgacatcgct acgcccgact ccgccgtgcg
     5521 agtactcgtc gttcagacca gagaggactg ggagatcgca cgggagtgct tccggctatg
     5581 ctcgcatgcg acgccgcgcg aaagctgcga agagaatccc gccaattagg gcgctccaac
     5641 tcacgatccg agcgacgcgc atttccatgc ctccgtcgta caccagttcc accgtacacg
     5701 gcccctcgca gccgggctca acaacgatct ggccgagagc atctccgtag aggcgcaccg
     5761 gtgcgcccgc taccgtggcg ctccaaccgg ggtggtagct gatctggaca gacaatacct
     5821 gaccgggttc tatcgacgcg gagacgacgg ccgaatgccg gctgctccac ttcatttccg
     5881 ccgccgggag tgctggatcg tcgagagccg ccacgtacgg ccgcatcgga tcgagatcca
     5941 ggccgtggat cggcgcgcgg gcgggcaggt cggcacggcg gatcacatgg gcgagagact
     6001 tggaccgccg cccgacgtcg tagataacgt catcagcgtc tcgccagagt tcccgcagca
     6061 ggccatcaaa cttgcgcgga tttcggaatg gcttgaagac ttcacggctt cgcggaccgc
     6121 tgacgccgac ggcgtccacg ccgaaggcct tgagagtggt gacggcgacc gtcccttcct
     6181 ggtttcccgc attctcgccg gtaaggagtt ggtagtgaaa ggcggcgtag ttcggattca
     6241 cgatgccctg atcgaacccg cccgcgaact gaggggtgtc tgtgaatacg ttgaggaaga
     6301 acccaaccga accgggcaac ataacacggc gtccggccat gtttcgctcg gcccagcgcg
     6361 cctcgcgata ctcgatccgc tgcgtgatat cgacggggcg aatcagccgt ttgccgcact
     6421 tacggtagcg gatggccggg tagacgcaga gaacaaccag caggcaggcg gcggcgaccc
     6481 gcaggcggcg cgacgaacgc tccagaaggt atcgcgcgcc gaagaccccg gtgagcacga
     6541 gcgccatttc catttccagg tgatagcggt gcccctgtgg catcagcgag accttccacc
     6601 attcggacgc cagcgtgaaa gcggccagcg gcgtcaggaa cagggcggca aagcggagct
     6661 ggggcggggc attcacgcgc cggaggagcc acaggagcgc agcggcgcca agcaccatca
     6721 acgccagata gagccagcgc gagggaggca aggtaccgct caaatagcgt tcgttccggg
     6781 agatcgcaag cagcgtggac ggaggaagcc aggaacatgc gagggcgtat gcgtacagcc
     6841 cgagcatggc tgcgcggagc catgtcccca ggcggatctc gtcgcttgcg aacagccaag
     6901 ccacgactgc gagcgcgagc gaggccgcgc cgagataatt agtcagggcc accgacgcga
     6961 gaccgaggga cgccgcgaac gaccacgccg ggtgacgtcg ttcgaccgcg acgatcacga
     7021 ggagcagcac aaggggaagc aacgtcatcg acgcgatgtg tggcccttcg ccgtattgca
     7081 ccagcgtctg aagacgccgg gcgccccaca cgcctccggc atcgtaccga acgattggga
     7141 tcaggagcgt ggatggggag aaaacggagt aaactaatcc ggccaaaaat ccgatgccta
     7201 ccgagcgcga cagccaggcg gcgaacagga agagtgtgac cggaccgaga ctgtagaaaa
     7261 ccgcggtgac ggcgtggtat gcgcgcgccg gcgggacatc ccctacgctc gagaccagac
     7321 cgacgatcca gtgatgcaga ggcgggtaag tgttctggta gggaatgcct ccgtaccaga
     7381 gcgggaacca ggaaaggtca cgccagttct cgctgaccca gcggccgagc gcgatgtgcg
     7441 tcccttcgat cgagttcatg tgatcgacga actcgaatcg gaacaggcgg ccggagatcc
     7501 acacgttgag cagaaacagc agcagcgccg caaggatcag ctttttccgg ctcatccggc
     7561 gcccaggtag aggaagcgcg cgataaacag cgccgtcagg atgtagacaa gccagctcac
     7621 tgtccggaac tcgccgcgta ggaccttcag cagcgtgtag gcggtgaatc cgaacgccaa
     7681 accgttggcg atgctaaagg tcagcgggat ggcggtcatg gtgaggaagg ccggcactgc
     7741 gatcatcggg ttcgaccagt cgatttcgct gatgtgcgac accatgagcg agccgacgat
     7801 gatcagcgcc ggcgcggttg ccgccgcggg aatcacgccg accatgggcg cgatgaacaa
     7861 ggcaacgacg aacagcaggc cggtgacgac ggaggtcacg ccggtccgcc cgcccgcgac
     7921 cacgccggcc gcgctctcga tgtaactgac aacggtggag gtgccggcaa gcgacccggc
     7981 agtgcttgcc acagcgtccg agagcaggat gcggttgatc cgcgggatct catgcgcctc
     8041 gttgaatagg cctgccttct tgccgacggc gacgagcgtg ccgacgttgt cgaacacatc
     8101 cacgaagaag aagacgaaga cgatctcgat gaagcccaga ccgaaggcag acgagaggtc
     8161 cagccggaac gcggtggccg aaacgtccga gagccggtac atctgcggct gccagtgcac
     8221 gagtccagct gccgcgccag cagcggtcgt cgcgagaatg ccgatcagca tcgccgcccg
     8281 cacgcgccag gcgagcaagg cggcgatcag cagcaagccg aacagggcca gcgctgtccg
     8341 gggttcaccg aggttaccga gtgccaccgt ggtctcttta ctcgccacga tgatgccgga
     8401 gttccggaaa ccgaccagtg cgatgaagaa cccgatgcca gcggcgattg cggagtaaag
     8461 ctcggtggga atcgacgcaa ctacgcgccg gatcacgccg cagagtgtga gcaccgcaaa
     8521 ggccacgccc gacaggaaca ccgcgccgag cgcggcctgc cacggcagac ccatcccctt
     8581 cacgaccgcg tacgcgaagt atgcgttcag tcccatgccg ggcgcgagtg cgatcgggta
     8641 acgggcgtag gcgcccatga gcaaactgcc gagagccgag gagagacagg tggccgcgac
     8701 taccgcggcg aacggcatcc cggcctcgtg caggatggag gggttcacga agatgatgta
     8761 ggccatcgtg ataaaagtgg tcacgccggc caggatctcc gtcttccaat tcgcgccgag
     8821 agtctggaat tcaaagtagc gctcgagccg cccgagaagt gtgctcatga atgggagatc
     8881 cccattctat agcagccagc ctgttacggg acccacttct cctcgcgcca cgccatgtcc
     8941 cagaacatgt actcgtagcg ggccgacagc acgaagaggt gtttggccgc ctgacggg
//
LOCUS       DATIFQ010000236         4848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2757961, whole genome shotgun sequence.
ACCESSION   DATIFQ010000236 DATIFQ010000000
VERSION     DATIFQ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757961"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="VLE22_10985"
     CDS             complement(<1..1528)
                     /locus_tag="VLE22_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14975.1"
                     /translation="MATLTPRLLLALSWIIGFAMAQPQPPVVIDGQLDDALWDTVKPE
                     KLAPAEAGVPAETGGEIRATIIGRYLCVAGRLPEPTGSVVARSIGRNPHWEEEDVLRI
                     VVGAYPDWTLQIGPFGAYSLETKGFVVPAERFVAAARLGEKEWRVEAAIPVNKLKAAR
                     LDEIRVSIVRIRAMRPGCPEQRWRWPEFEPAGKVPAHPSYNWDAPAPLFRPALIGNTE
                     PPLQVGSTKEIPPLTAGWSDPPWRDVPAWSLVRNEPSARLPQTPTEVKLLHDRITLAV
                     MARCSEPDAVMARAAENDGPVDRDDSFQIYLAISGSSYVQFAINPRGFVLDAAGKSGG
                     PRVSRPRPEWNSQVRKAVLLEQRTWTVRLDIPLDQVATVLGEEQIPPEWRVLLRRFRP
                     GRPDEPPETSVLPVVESDTPLCPARYRRLVLANRDSVQSPGASQPAGILAPFETRVLS
                     GEQRKATRLAEMLDRHLQSRIEKAVEADKKEWERVKTLADWERFRDPRLQALRASLGE
                     F"
     gene            1610..1831
                     /locus_tag="VLE22_10990"
     CDS             1610..1831
                     /locus_tag="VLE22_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14976.1"
                     /translation="MVSFYAAIRPRYGAGPKTALCAGLAVWFLGYLLASVAPLVLDLF
                     PARVMVVGLVVGLVDALMATLLGARLYQE"
     gene            complement(1971..3260)
                     /locus_tag="VLE22_10995"
     CDS             complement(1971..3260)
                     /locus_tag="VLE22_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14977.1"
                     /translation="MTEAVKIKNTTMQAAFADSSDRSPAPAVISPMDEHMGLDIARSL
                     GRRGVPVYGMDADPRAPGGKSKYVHLLRCPDPHRDEEKFVDFLVAWGKESGVKAVLFP
                     VSDEVALLCSRERERLSPYYAFVMPDHETLVSLSTKAGLGRVADSLGVPAPKTFFLDD
                     EQSLAEVARQVEYPVLLKPVESHFWHRPEIAAQLLGQFVDGRAKVAVCSDPVILERTY
                     RGIARYDARMVVQELIPGPDENLTYISFYLNRESEPLALFAGRKLRVIPLGFGSASYV
                     RSFHDPGLVEVALRLLRGAQYQGLGGLEFKKDSRDGRYKLIEFNTRFGMWDGLGVRCG
                     VDTPYIAYCDALGRRSEPVFNYREGVIWWDWQRDVRAFWMLRKRKQLTLKEWLGSLRG
                     EKMWAIYSLDDWLPGFAFTWSLVRILSERAWGRLTGR"
     gene            complement(3322..4389)
                     /locus_tag="VLE22_11000"
     CDS             complement(3322..4389)
                     /locus_tag="VLE22_11000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB14978.1"
                     /translation="MKPLVEAAPRDTSLFTQSQTPPSIVNTLPCSRWSRFVAEHPDGN
                     IFNTPEMFEVFRHSRGYDPELWAAVDGARILALFVPVRVSLCDGPLRPFVTRAIAYGG
                     VLAAPGEEGAWAVTRLLKTYKRHVSGVPLFTELRHLSDSSALQSVLKDRGFSFSPQMN
                     YLVDLARPPEAIMASISYRTRRHIRHGLRKGTVTIREAEGPEQIAHCYDLLSRTYKAA
                     GVPLADRSLFDAACKVLRPKNMIRCTLAWVGSTPVAVSIDLLYRETVYGWYGGVDRAF
                     GADNPNELLTWHVFEWGSTHGYRRYDWGGAGVPGKAYGVRDFKSKFGGEEVSFGRNVC
                     IHAPFRFRLSSAVYKVCRRWL"
BASE COUNT          985 a         1511 c         1421 g          931 t
ORIGIN      
        1 gaaactcgcc cagggacgct cgaagcgcct gcaagcgcgg atcgcggaat cgttcccagt
       61 cggccagggt tttcacgcgc tcccactctt tcttgtccgc ctccacagcc ttctcgatac
      121 ggctctgcag gtggcggtca agcatttcag cgagacgggt cgctttgcgc tgctcccccg
      181 ataggacgcg cgtctcgaac ggcgcgagta tcccggcagg ttggctggct cctggcgatt
      241 ggacagaatc gcggtttgcg aggacgaggc gccggtagcg ggccggacac agaggtgtgt
      301 cactttcgac gacgggtagg acgctcgtct cggggggctc gtcgggacgg cccgggcgga
      361 agcggcgcaa caggacacgc cattcgggcg ggatttgctc ttctcccaac actgttgcga
      421 cctgatcgag cgggatgtcc aggcgcacgg tccatgttct ctgctccagg agcaccgctt
      481 tgcggacctg gctgttccac tcgggccgcg gccgggaaac tcgagggcct ccagacttgc
      541 ccgccgcgtc cagtacgaag ccgcgcggat tgattgcgaa ctgaacgtaa gaagagccgg
      601 aaattgcgag gtagatttga aagctgtcgt cccggtccac tggaccgtcg ttttccgcag
      661 cccgcgccat gacggcatcg ggttcggaac acctcgccat gacggccaac gtaatgcggt
      721 cgtgcagcag cttgacttcc gtaggcgtct gcgggaggcg agccgacggc tcatttcgaa
      781 ccaggctcca ggcgggaacg tcacgccacg gaggatcgct ccatccggca gtcagcgggg
      841 ggatttcttt agtgctcccc acttggagcg gcggttccgt atttccgatc aaggctgggc
      901 ggaacagagg cgccggcgcg tcccagttgt aggacgggtg tgcgggcacc ttccccgccg
      961 gctcgaactc aggccaccgc catcgctgtt cggggcaacc agggcgcatc gcccggatcc
     1021 ggacgatgct tacgcggatc tcgtccaggc gcgcggcctt tagcttattg accggaatcg
     1081 ccgcttcgac ccgccattct ttttcgccga gtcgcgcggc ggcgacgaac cgctcagccg
     1141 ggaccacaaa tcccttggtc tcgagagagt acgcgccgaa cggaccgatc tgcaaggtcc
     1201 agtcaggata ggcgccaacg acgatccgga gaacatcctc ctcttcccag tgcgggttcc
     1261 ggccgatgga gcgggccacg acactgccgg taggttccgg tagccgcccg gccacacaca
     1321 ggtaccggcc aatgatcgtg gcgcggattt cgccgcccgt ctcggccggc actcctgctt
     1381 cggcgggcgc cagtttttct ggcttgacgg tgtcccacag ggcatcgtcg agctgtccat
     1441 caatgacgac gggaggctgt ggctgggcca tagcgaaccc tatgatccac gagagcgcta
     1501 gaagcaatcg gggtgtcaga gttgccatct tgcacctcct tcgaacaagt tggacacgcc
     1561 agggtcagta ctgccgacca ggatatcttg atctgccccg aggtgggact tggtttcgtt
     1621 ttatgcagcc attcgcccgc gttacggcgc cgggccaaag accgcgctct gtgccgggtt
     1681 ggccgtctgg ttccttggct accttctggc ttcggtagcg ccgctcgtgc tggatctctt
     1741 tcccgcccgg gtcatggtcg ttgggcttgt ggtggggtta gtcgacgccc tcatggcgac
     1801 gctgctcggg gcgcggttgt accaggaata agatccagtg agctgtctta gcagggtgct
     1861 gaaaaaccga cgggtgttga cacccgacac agcctaaagg ctgtgccacc actgagaatc
     1921 aactcctacg acctgcggtg gcacaggctt cagcctgtgg cgcgggtttt tcagcgccct
     1981 gttagacggc cccacgcacg ttccgacaaa attcgcacca aactccaagt gaatgcgaac
     2041 ccgggcagcc agtcatcgag agaatagatc gcccacatct tttcacctcg caaagagcct
     2101 agccattcct ttagagtaag ttgtttacgc ttccgtaaca tccagaacgc ccgaacgtcc
     2161 cgctgccaat cccaccaaat cacgccttcg cgataattga acactggttc ggaccgccgg
     2221 ccaagagcat cacagtaagc gatgtaaggc gtgtcgaccc cgcagcgaac tccaagcccg
     2281 tcccacattc cgaagcgtgt gttgaattca atcagtttgt agcgcccgtc acgcgaatcc
     2341 ttcttaaact cgagcccgcc caagccctga tattgcgccc cacgaagcag tcgcagagcg
     2401 acctcgacga gtccgggatc gtgaaagctt cgcacgtagc tggccgagcc gaatcccaag
     2461 gggatcactc gaagtttgcg gcccgcgaac aatgccagag gttccgattc ccggttgaga
     2521 tagaaactga tgtaggtcag gttctcgtcg gggcctggaa tcagctcctg gacgaccata
     2581 cgcgcatcgt aacgcgcaat accccgatag gttcgctcca aaatgacagg atcggaacag
     2641 accgcaactt ttgcgcggcc atcgacaaac tgcccgagta gttgtgcggc gatctcaggc
     2701 ctatgccaga aatgactctc caccggcttc aggagaaccg ggtactccac ctgccgcgcg
     2761 acttccgcga ggctttgctc atcatcgagg aaaaacgttt tgggtgcagg cactccgagc
     2821 gagtccgcaa cccggcccag cccggctttt gtcgacaggc tcaccaacgt ctcgtggtcc
     2881 ggcatgacga acgcatagta ggggctgagc ctctcacgct cccgagagca gagcagcgca
     2941 acctcatcgc ttacgggaaa caggacggcc ttgacaccgc tctcctttcc ccaggcaacc
     3001 agaaaatcca cgaacttctc ttcatcacga tgaggatcgg gacaccgcag caggtgaaca
     3061 tacttcgact ttcctccggg agcccgcgga tccgcgtcca taccgtagac aggaacacct
     3121 cgccggccca gggaacgtgc gatatcgaga cccatgtgtt cgtccatcgg cgagatcacg
     3181 gcgggggccg gcgaacggtc ggatgagtcc gcgaaggcag cttgcatggt cgtgttcttg
     3241 atctttaccg cttcagtcat tcttgggtca accgcttgca ggcgctcgat cgggccgacg
     3301 tatccggaac tttcggcgta gtcagagcca ccggcggcaa actttgtaaa cagcgctact
     3361 caaccggaaa cggaagggcg cgtggatgca gacattccgg ccgaaactga cctcttcgcc
     3421 accaaacttc gacttgaagt cccgaacgcc atatgccttg cccgggacgc cagctccacc
     3481 ccaatcgtaa cggcgatagc cgtgcgtgga gccccactca aagacgtgcc acgtcagcaa
     3541 ctcgttcgga ttgtcggcgc caaacgctcg atccacgcca ccgtaccaac cataaaccgt
     3601 ctcccggtac aaaaggtcga tcgaaacagc gaccggcgtg gagccgaccc acgcgagggt
     3661 gcaccgaatc atgttcttgg gacgcaagac tttacaagcc gcatcaaaga gcgaccggtc
     3721 cgcaaggggg actccggcgg ccttatacgt ccgactcaac aggtcgtagc aatgagcgat
     3781 ctgctccggg ccctccgctt ctcttatggt taccgttcct ttgcgcaaac cgtgccggat
     3841 atgcctgcga gtccgatagc tgatgctcgc cattattgcc tcggggggac gcgcaaggtc
     3901 aacaagataa ttcatttggg gagagaagga aaagccacgg tctttgagaa ctgattgcag
     3961 cgcagaactg tcggagaggt ggcgcagttc agtgaacagc gggacgccgc tcacatgcct
     4021 cttgtatgtc ttcagcaagc gggtgactgc ccaagctcct tcctcccccg gcgcggccag
     4081 tacgccgcca taggcgatgg ctcgcgtgac aaaaggccgg agcggaccat cgcaaagcga
     4141 tacccgcaca gggacgaaca gcgcgagaat tcgtgcgccg tcgaccgccg cccatagctc
     4201 cgggtcatac ccgcgagaat gccgaaagac ctcaaacatc tcgggcgtgt tgaaaatatt
     4261 accgtcggga tgctcggcga caaaccgact ccatcgcgag cagggcaacg tgttcacaat
     4321 gctcggaggt gtctggcttt gcgtgaaaag actagtatcc cggggcgccg cctcgacaag
     4381 cggtttcatt cggttaattt ttgcgcctga atgaagcgaa gtcaatagtt ccgaccactt
     4441 ccaggacgag cagatgtact agaacaaaca acgggtgggt ctgggggtac cgaggttact
     4501 tgtgggaaca ctgagggcag gcttcgtgtt atcagtcgct tgcagcgggc gcagcagcta
     4561 tggacccgtc attccgcctg agacccgtca ttccgcctga agactcggaa ccaccttgag
     4621 ccacaaatgc ggctcctact agcgaagggt ttaacccggt ttaacagttc ccctctactt
     4681 gggtttcgcg tactgatcag cgcctgtgat gtccaggatg accacgggct cgttccccac
     4741 cacccaggcg tcatggccgg gcggtatcga acagacctcc ccggggccga tatcccgtgc
     4801 ggttccgtca tccatgacaa tgtgcattcg accggatacc tgatacgc
//
LOCUS       DATIFQ010000237         2988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2374535, whole genome shotgun sequence.
ACCESSION   DATIFQ010000237 DATIFQ010000000
VERSION     DATIFQ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374535"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VLE22_11005"
     CDS             complement(<1..768)
                     /locus_tag="VLE22_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14979.1"
                     /translation="MHGTRAAAAILFVSLSYWPRVSFGAEGADSDQLTGPHLGVLFDP
                     SLRRFHQIYGIPGSATLGSPMDVGVSVSRALVSPDGDHALVELREGREIGLIRFRDGR
                     AATTVLADAPQAPDLMALSPSGSAGALLYVTTGRIVVLSNLGMAHAQAREVAFPFGLA
                     RPGLLAVSDDGGALLAVVPDGDEDSLYTAAGDDLRLLGRFGRVTSATFVAGARHALVA
                     DALVNRIYRISAAGAGGQLTPLADERQGISGPVAVAAS"
     gene            complement(792..1730)
                     /locus_tag="VLE22_11010"
     CDS             complement(792..1730)
                     /locus_tag="VLE22_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14980.1"
                     /translation="MFLAVPAAVHGVSSDTSSEQTGAAADGNYETSVNAASEGCGSLT
                     AAPVITAVVNAGSYRQQDGIAMNTMLAIFGSGFAPPGTSRVVGTSDILENRFPETLAC
                     VGVEIAGQTAIMAYISDTQINVQAPTNLLFGPAEVEVRLNKGQPNEVKGKFSGVQVRT
                     YSPAFFQFLPSLSIAAQIAPSYELLARPEVHPQGRAVKPGEVAILYGTGFGPTVPVWP
                     AGEIPAFASKIRDPMTLRIGGVLLAQDDIIYAGLSPGSISGLYQINVRVPATTPDGDI
                     PVEVEIGGMRTQPGAFIPVRQVTTISPGTPIVGPPQ"
     gene            1947..2846
                     /locus_tag="VLE22_11015"
     CDS             1947..2846
                     /locus_tag="VLE22_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSB14981.1"
                     /translation="MANTARPTESGEKRSAALNSLIAAVALTAFKVTVGLLTGSLGIL
                     AEAAHSGLDLVAAGMTFLAVRFAGRPADRSHLYGHGKIENLSALFETLLLLITCAWIV
                     WEAVHRLASSRIDVEVTAWSFIVMITSIVIDYSRSRVLTRVAKKYHSQALEADALHFR
                     TDIWSSGVVILGLVSVKLGEWLPSLGFLSHGDAVAALGVSAVVVWVSVQLGRQTVDAL
                     LDTAPAGMEEQIAAVVAAVPGVLDCHNIRIRYSGPVLFVDLHVLVDGGQTLSSVHGLT
                     EVIEVAIQEAIPRADVTVHPEPF"
BASE COUNT          564 a          941 c          914 g          569 t
ORIGIN      
        1 ggatgcagcc acggcaaccg gtccggagat cccttgcctc tcatccgcga gcggggtaag
       61 ctgcccacca gcgccggcgg cgctgattcg atagatccgg ttaacgagcg catcggcaac
      121 aagggcatgc cgggcgccgg ccacaaacgt cgcggaagtg acccgcccga aacggccgag
      181 caggcggagg tcatcgcccg cggccgtgta gagggagtct tcatcaccgt ccgggaccac
      241 cgccagcagg gcgccgccgt catcgcttac ggccagcagg ccgggccggg ccagtccaaa
      301 tgggaacgcg acctcgcggg cttgcgcgtg cgccatgccg aggttggaca agaccacaat
      361 tcggccggtt gtaacgtaca acaaagcgcc ggcgctgccg gacggactta aggccatcag
      421 atcgggggcc tgtggagcat cggcgagcac cgtcgtcgcc gctcggccat cgcgaaaacg
      481 gatcaatccg atttcgcggc cttcccgtaa ctcgacgaga gcatggtcac cgtccgggga
      541 cacgagggca cgggagacgg agacgccaac gtccataggc gaaccgagcg tcgcggaacc
      601 gggaatcccg taaatctgat ggaaccgccg gagggacgga tcgaaaagta cgcccaagtg
      661 tggcccggtc agttggtctg agtctgcgcc ctcggccccg aatgacacgc gcggccaata
      721 ggaaagactc acgaagagaa tggcggctgc ggcacgtgtt ccatgcatgg cgctcctccc
      781 gcttgctcga gtcactgtgg cgggcctaca atcggcgtcc cgggagaaat ggtagtgacc
      841 tgtctcaccg ggatgaatgc ccccggctgc gttctcatgc cgccgatttc gacctcgacg
      901 gggatatcgc cgtccggtgt cgttgctgga acgcggacat taatctgata cagcccacta
      961 atcgatccag gcgacagacc ggcgtagatg atgtcgtcct gcgccaaaag caccccgccg
     1021 atgcggagcg tcatcgggtc ccggatcttc gatgcgaacg cggggatctc gcccgcaggc
     1081 cacaccggga ccgtcggacc gaaacccgtc ccgtacaaga tcgccacctc acccggcttc
     1141 acggcgcggc cctgcggatg gacctcgggc cgggccaata attcgtagct cggggcaatt
     1201 tgcgccgcaa tgcttaaaga cgggagaaac tggaagaacg ccggcgagta tgtccgtacc
     1261 tgaacaccgc tgaacttgcc cttcacttcg ttgggttgtc ccttattcag cctcacctcg
     1321 acttcagccg gaccgaacaa aaggttggtg ggcgcctgca cgttaatttg agtgtcggag
     1381 atatacgcca taatcgcagt ctggccggcg atctccacgc ccacgcaggc aagtgtttcg
     1441 gggaacctgt tttccagaat gtcggatgtc ccgaccacgc gggacgttcc cggcggcgcg
     1501 aagccggaac cgaaaatcgc cagcatcgtg ttcatcgcga tgccgtcctg ctggcggtag
     1561 gatccggcgt tgaccaccgc ggtgatcacg ggcgcggccg tcaggctgcc gcaaccttcc
     1621 gaagcggcgt tgacgctggt ttcgtagttg ccatccgccg cggcgccggt ctgttcggag
     1681 gacgtatcac tgctcactcc gtggacggcg gccggtaccg ccagaaacat cgcaagctgc
     1741 agaattcgcc cagcacgagc cgttacgcga atggaatggc atctcttcat gcggcaccga
     1801 cggcccgacg gcccgaccgc cccttctctc cccgagcggc agtgaacggg gccttcgaac
     1861 agcataatac acccagacgc catcatttga atgtgaaaac tgcggtattc cgtcatggaa
     1921 gtcgactgtg atagctttac ctccagatgg cgaatacagc ccgtccaacg gagagcggcg
     1981 aaaaacgttc cgccgctctg aactcgctca tcgccgccgt tgccctcacg gcgttcaaag
     2041 tcactgtcgg tcttcttacc ggtagcctgg ggatccttgc cgaggcggcc cactccggtc
     2101 tcgacttggt agctgccggg atgacctttc tggcagttcg gttcgcgggc cgccctgctg
     2161 atcgcagtca tctgtacgga cacggcaaaa tcgaaaacct gtcagcgctg ttcgaaactc
     2221 tgctcttgct catcacctgt gcctggatcg tgtgggaagc ggttcaccga ttagcatcaa
     2281 gtcggatcga tgtcgaggtc accgcatggt cgttcatcgt gatgatcact tcgatcgtga
     2341 tcgactactc ccgatcgcga gtgttgacgc gggtggccaa gaaataccat agccaggccc
     2401 tcgaggcgga cgcactccat ttccggaccg atatctggag ttccggagtc gtcattcttg
     2461 ggctcgtttc ggtcaagctg ggcgagtggc ttccatccct tgggtttctc agccacggcg
     2521 acgccgtggc tgcgctgggc gtctcggcgg tcgtagtgtg ggtgagcgtg caactcggcc
     2581 gtcaaacggt cgacgctctt ctcgacactg ctcccgcagg tatggaagag cagatcgctg
     2641 ccgttgtcgc ggccgtgcct ggtgtcttag attgtcacaa catccggatt cggtactccg
     2701 gtccggtcct gttcgttgac cttcacgttc tcgtggacgg tggccagacc ctgtcgagcg
     2761 ttcacgggct tacggaagtg attgaggttg cgattcagga ggccatcccg cgggccgatg
     2821 tcaccgttca ccctgagcca ttctgagcgg cgcggcgccg actaccgcca cgctccacta
     2881 ccctcaagag gttgtatatg cgaatgattg cactgtgttt cttcgcgctt ctgcgcttcg
     2941 ttgttgttga cccaacagca gccgcccccg aggacaccgg catgaagg
//
LOCUS       DATIFQ010000238         7123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_652675, whole genome shotgun sequence.
ACCESSION   DATIFQ010000238 DATIFQ010000000
VERSION     DATIFQ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652675"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..90)
                     /locus_tag="VLE22_11020"
     tRNA            complement(1..90)
                     /locus_tag="VLE22_11020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Ser,seq:cga)
     gene            complement(86..853)
                     /locus_tag="VLE22_11025"
     CDS             complement(86..853)
                     /locus_tag="VLE22_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14982.1"
                     /translation="MTGFRPEFLDFRRGIRVGNLEDNERITRILKLGLESRYGEPFIT
                     ERYGRGVYWHWIGYVARSNREAKPLSSHVSFGCSKFFLAVDTGERLFSCGMQVERGFV
                     RASHDAKGWELQSDWDWHRLWNALTPKGALDREIRRLLREGFRVHAGSWDEDSAHYSK
                     SDYPGAAKLRRVMDAAPKDRWAGFQLYYPMTEKEVQASTGPDLVDAMLAVFGEVTEAM
                     NLSMQVELGWNNAGTHLEKAHPVGYSGERDLPSTSSR"
     gene            1021..4119
                     /locus_tag="VLE22_11030"
     CDS             1021..4119
                     /locus_tag="VLE22_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSB14983.1"
                     /translation="MLYWIEDTWDNRFKPRLQELVETSASRRRVPFRLPRFSIGRPRA
                     GRIWATSIGISLILIGAEMRTSWLQSWVLSTLAGRLSYAVAPGPSDAIRFPQAGPYND
                     RLGYSRLPDFNARLKSAGCRIDAQARNSHLAVTLADLGLYPVYPEKTQAGLQILGRDR
                     RPLFSSKYPGRAYATFEAIPPLVVQSLLFIENREILESSYPYRNPSVEWDRLAGAVVD
                     LGLSAVSSHRLSGGSTLATQIEKVRHSPGGRTASVTEKLRQMASASLRAYQGGQETIE
                     ARRRIVRDYVNSVPFSAIRGYGEIHGLGDGLWAWYGADFKEVNHLLAGAASSGNIAAK
                     GLACRQALSLLLAINSPSTYLRADPKALEARTDAYLHLLAKEGLISNALRDAALRARA
                     GIRTVAPPRPPVSFADRKGSDPVRVRLLSLLGLESTYVLDRLDLSVRTSLDGPAQDVI
                     THSLQRMTDPAYAAQAGLVGERMIQSGDPSSVIYSFSLYEHSGGVNLLRVQADNYDHP
                     LSINEGTRLELGSTAKLRTLVNYLSIVAGLHREYAPLTTTRLRELAAAKHDRLTAWAI
                     DYLASAPGASLEAMLEAAMSRHYSANPGESFFTGGGLHQFSNFDKKDNGRIMTVSEAF
                     DRSVNLVFIRLMRDIVNYYVRRLPSYSPVIFEDRSLPVRRQYLERFADMEGKQFLAKF
                     HEKYRDQIPDESLELLVMGIHPTPVRLAVIYRSLRPEAGVGEFAAFLRTHLPNPSLRG
                     GAVETLYRKYARDNYSLADRGYLAGVHPLELWLLEYLRHYPGASLAEVFAAGALARQQ
                     AYEWLYKTRYKGGQDQRIRIMLEIDAFDAIHKEWKRQGYPFQNLTPSYAAAIGSSGDT
                     PAALAELAGIIMNDGVRYPTVRIQRLHFAAGTPVETILSPSLPAAEKVYPSAVAAVAR
                     RAMVGVVERGTGRRAYHSVALPGGRMLEVGGKTGTGDNRFEVYGPGGRPIDSRVLNRT
                     ATFVFFFGDRFFGVITAFVPGEKAGQYEFTSALPVQAFKHLAPELIPLVEKHDEALLA
                     RR"
     gene            4218..5753
                     /locus_tag="VLE22_11035"
     CDS             4218..5753
                     /locus_tag="VLE22_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSB14984.1"
                     /translation="MSIRTNIQEVLTRLEIAPVNSGACWGIWIPDPAGGEMASVDPTD
                     GSEIARVKMAGPDDYEQVIRNAAAAFLDWRHLPAPKRGQIVREIGDELRRNKDDLGAL
                     VSLETGKIIAEGKGEVQEMIDIADFALGLSRQLYGPTMASERPAHRMYEQWHPLGVVG
                     VVTAFNFPVAVWSWNAFIAAVCGDTVVWKPSSDTPLTAIAVQKICNRVLERHGWKGVF
                     NLVVGKGSSVGERLLADRRVPLVSATGSCHMGYRVAEVVSKRLGRTILELGGNNGVIV
                     MDDANLDLALRAILFGAIGTAGQRCTTIRRLFLQKGIAKEMTDRLTAAYRQVRIGDPL
                     DESVLMGPLVNRKSVEEMQRGLDTIRSQGGEILCGGKALDRPGGCFVEPALVRARPDM
                     PVLRDELFAPVLYLIEFDSLEEAVRWHNDVPQGLSSAMFTTSLMSAEIFLSTRGSDCG
                     IANINIGTSGAEIGGAFGGEKETGGGREAGSDAWKQYMRRQTVTINWSTNLPLAQGIK
                     FEL"
     gene            5787..6590
                     /locus_tag="VLE22_11040"
     CDS             5787..6590
                     /locus_tag="VLE22_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14985.1"
                     /translation="MPIYTCEDMIRDCRAGKTEGWSYFVQQYVPVVRRLADHYYPERA
                     GDTDLSARVLQTLGKPDLPLFVAAGPLSERDFVADLRQFVLGAIEEDSASDEAEISVD
                     VETLASALESLSTIEKQVAWLEAMRYGADETAHMMAMHASTVANIRERAAELLRSRVS
                     AWQAGMIAGNGPALRHEAVLLGKDDCPSARVWLDWLDGRITWYQREDAERRLAACWHC
                     IDHFSRLREAQSLLRELKPLTAAEAEPFRRAFGLQSVKPAFWKRFGSRE"
     gene            complement(6606..>7123)
                     /locus_tag="VLE22_11045"
     CDS             complement(6606..>7123)
                     /locus_tag="VLE22_11045"
                     /inference="COORDINATES: protein motif:HMM:NF020952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HSB14986.1"
                     /translation="TGLIKANFPARISFRVATRVDSRTILDVMGSEHLLGKGDMLFLP
                     PGSSRLIRVHGALVTETEINRVVDFWKQQARPEYDATFLVAPPSEDDEADLENLDGEQ
                     DPLYEDAVRVVLEMGKASTSTLQRRLRLGYGRAARILDMMQHDGIIGPPDGSKPREVL
                     KRPEWLDEVRA"
BASE COUNT         1262 a         2435 c         2126 g         1300 t
ORIGIN      
        1 tggcggtgag ggtgggattc gaacccacgg aacccgtaaa ggttcaacgg ttttcgagac
       61 cgcccgattc aaccgctctc gcacctcacc ggctcgatgt ggaaggtagg tctctttctc
      121 cactatagcc gactggatgc gccttttcta gatgtgttcc cgcgttgttc caccccagtt
      181 ccacctgcat cgagaggttc atcgcctccg tcacctcccc gaataccgcc agcatggcgt
      241 cgacaaggtc cggcccggtc gacgcttgaa cctctttttc ggtcatcggg taatacaact
      301 gaaatcctgc ccagcggtcc ttcggagccg cgtccatcac ccggcgcagc ttcgcggcgc
      361 caggatagtc cgacttcgag tagtgggccg aatcctcgtc ccagcttccg gcgtgcaccc
      421 ggaacccctc gcgcagcagc cgccggatct cacgatccag agcgcccttt ggcgtcaaag
      481 cgttccagag ccggtgccag tcccagtcgg actgcagttc ccagcccttc gcgtcgtgcg
      541 acgcccgcac aaacccccgc tccacctgca tgccacaaga aaacaggcgc tctccggtgt
      601 ccaccgccag gaagaacttg gagcagccga agctgacgtg cgacgacagc ggcttcgcct
      661 cccggtttga tcgcgccacg tacccgatcc aatgccagta cacgccgcgt ccgtatcgct
      721 ccgtgatgaa cggctccccg tagcgggact ccagacccag cttcagaatg cgcgtgatcc
      781 gttcattatc ttcgagattg ccgacgcgga tgccgcgccg gaagtccagg aactccggtc
      841 gaaatcctgt caccactgtc gttcaccgtc catctaagtc tactcctcgc cagtcgcagc
      901 ccggaagaat ggcagggaat cctgaaacgc tggccggttg cgctcactat caaagtagag
      961 gcgcaatttg taccttctag accggctggc gcatctacat cggtaaaggg ggtcgcagcg
     1021 gtgctgtact ggatcgaaga tacctgggac aaccggttca agccgaggct gcaagagcta
     1081 gttgagacat ccgcttccag gcggcgggtt ccgttccgct taccccggtt ttctatcggc
     1141 cgtccacgcg ccggccggat ctgggctaca agcatcggca tctcgctcat cctgatcggg
     1201 gcagaaatgc ggacttcgtg gctccagtcg tgggtgctct ccacactcgc gggccggttg
     1261 agctacgccg ttgcgccggg accgagcgac gcgattcgct tcccccaagc aggcccctac
     1321 aatgatcgcc tcggttactc ccggctcccc gacttcaacg cccgcctgaa gtcggccggc
     1381 tgccggatcg acgcccaggc tcggaattcg cacctcgccg tcaccctggc tgacctgggt
     1441 ctctaccccg tctacccgga gaagacccag gccggactcc agattctcgg gcgcgaccga
     1501 cgcccgctgt tttcctcgaa ataccccggc cgggcgtacg ccacgtttga ggcaatccct
     1561 cctctggtcg tccagtcgct gctgttcatc gagaaccgtg aaatcctcga gagcagctac
     1621 ccctaccgta acccgtccgt cgagtgggac cggctggccg gcgccgtcgt cgacctcgga
     1681 ctgagtgccg tcagcagtca ccggttgagc ggcggcagca cactggcaac gcagatcgag
     1741 aaggtccgcc attcgccggg tggccgcaca gcatcggtca ccgagaagct tcgacagatg
     1801 gcctctgcct cactccgcgc gtaccaggga ggccaggaga cgatagaagc ccgccggcgg
     1861 atcgtccgcg attacgtcaa ctccgtcccg ttttcggcca ttcgaggcta cggggagatt
     1921 cacggcctgg gcgacggtct ctgggcttgg tatggcgcgg acttcaaaga ggtgaaccat
     1981 ctcctcgccg gagcggcttc ctccggtaat atcgccgcca aaggccttgc ctgccggcag
     2041 gccctgagcc tgttgctggc aatcaacagc ccctcgacgt acctccgggc cgaccccaaa
     2101 gcgctcgaag cgcgcaccga cgcgtatctg cacctgctag ccaaggaggg ccttatctcg
     2161 aacgcgttgc gcgacgcggc tctccgcgca cgcgccggca tacgcactgt ggcgccgccc
     2221 cgcccgcccg tctccttcgc cgacaggaag ggatcggatc cggtccgcgt ccgcctgctg
     2281 tcgcttctcg gcctggagag cacctacgtt ctggaccgcc tcgacctctc ggtccgtacg
     2341 tccctcgacg ggcctgctca ggacgtcatc acgcactctc tccagcgcat gaccgacccg
     2401 gcctacgccg cccaggccgg tctggtcggt gaacggatga ttcagagcgg cgacccctcg
     2461 tcggtgatct acagtttttc gctttacgag cactccgggg gtgtgaacct gttgcgtgtg
     2521 caggctgaca actacgacca tcccctcagc atcaacgagg ggacccggct ggaattggga
     2581 tccaccgcca agcttcgcac cctggtgaac tacctctcaa tcgtggccgg actgcatcgc
     2641 gagtacgcgc cgctcaccac tacccgactg cgggagcttg ccgcggcgaa gcacgacaga
     2701 ctgaccgcat gggccattga ctaccttgcc tcggcgcccg gtgcgagtct ggaagcgatg
     2761 ctcgaagcgg ccatgagccg ccactattcg gccaatccgg gcgagagctt ctttaccggc
     2821 ggcgggctgc accagttctc gaacttcgac aagaaggaca acggccgcat catgaccgta
     2881 tccgaggcgt tcgaccgctc ggtcaacctc gtcttcatcc ggctgatgcg cgacatcgtg
     2941 aactactacg ttcgcaggct cccgagttat tcgccggtga tctttgaaga ccggagtctc
     3001 cccgttcgcc ggcagtatct cgagcgcttc gctgacatgg agggcaagca atttctggca
     3061 aagttccacg aaaagtaccg cgatcagatc ccggacgaat cgctggaact gctcgtgatg
     3121 ggcatccacc cgaccccggt gcgcctggcg gtgatctacc gttcgctgcg gccggaagcc
     3181 ggcgtcggcg agtttgccgc ctttctgaga actcacttgc cgaacccgag tctgcgcggg
     3241 ggggccgtcg aaactctgta ccggaagtac gcccgcgaca actatagcct ggccgaccgc
     3301 ggctacctag ccggcgtgca tccgctggaa ctgtggttgc tcgaatatct gcggcattac
     3361 cccggagcga gcctagcgga ggtctttgct gccggcgccc tcgcgcgtca gcaagcctac
     3421 gaatggcttt acaagacccg ctacaagggt ggacaagacc agcgaatacg gatcatgctc
     3481 gaaatcgatg cattcgacgc gatccacaag gaatggaagc gtcagggcta cccgttccag
     3541 aatctgacgc cgtcctacgc agccgccatc ggcagttcag gcgacacgcc cgcggctctc
     3601 gccgagttgg ccggcatcat tatgaacgat ggcgtgcgtt atccgacggt gcgcatccag
     3661 cggctccatt tcgccgcggg gacccccgtc gagacgattc tctcgccgtc cctgcccgcc
     3721 gccgagaagg tgtatccgtc tgccgtagcg gccgtggcgc gccgcgcgat ggtcggcgtt
     3781 gtcgaacgtg gcacgggacg aagagcgtat cattctgttg cgctgcccgg tggccgcatg
     3841 ctcgaggttg gcggcaagac cggcaccggc gacaaccgct tcgaggttta tgggccgggc
     3901 ggccgcccga tcgattcacg agtcctcaac cggaccgcca ccttcgtctt tttcttcggc
     3961 gaccggttct tcggagtcat caccgccttc gtccccggag agaaagcggg ccagtacgag
     4021 ttcacaagcg ccctgccggt gcaggcattc aagcacctcg ctccggaact gattcccctc
     4081 gtcgagaagc acgacgaagc gctacttgcg cgccgctgac cgcggatcgc ctccaagtgt
     4141 aagaatgagg gactgacctt cgatctctcc aacattgtgg aatcggaggc tggacccagt
     4201 tctgcccgag aggaagcatg tcgatccgca cgaacatcca ggaagttctc acacgtctcg
     4261 aaatcgctcc cgtcaactcc ggcgcttgtt ggggcatctg gatccccgat cccgcgggcg
     4321 gagaaatggc gtctgtggac cctaccgatg gaagcgagat cgcgcgggtg aaaatggccg
     4381 ggccggatga ctacgagcag gtcattcgca acgccgcggc tgcctttctc gactggcgtc
     4441 accttccggc gcccaagcgc gggcagatcg tgcgcgaaat cggcgacgag ttgcgccgca
     4501 ataaggacga cctcggcgcc ttggtctctc tcgaaacggg gaagatcatc gccgaaggaa
     4561 aaggcgaagt ccaggagatg atcgacattg ccgacttcgc tctcggcctg tctcgccagc
     4621 tctacggccc tactatggcg agcgagcgcc ccgcgcaccg gatgtacgaa cagtggcatc
     4681 cgctcggggt cgtcggcgtt gtgaccgcgt tcaacttccc ggtcgccgtc tggtcgtgga
     4741 atgcgttcat cgccgctgtt tgcggagaca cggtcgtgtg gaagccctct tcggacacgc
     4801 cgctgaccgc catcgccgtc cagaagatct gcaaccgcgt gctggagcgc cacggctgga
     4861 agggcgtctt caatctggtc gtcggcaagg gctccagcgt cggcgagcgg cttctcgcgg
     4921 atcgccgcgt cccgcttgtc agcgccaccg ggtcctgtca catgggttac cgtgtcgccg
     4981 aggtcgtctc caaacgtctc ggccgcacca tcctcgagct cggcgggaat aacggcgtga
     5041 tcgtcatgga cgacgccaac ctcgaccttg ccctgcgcgc catcctcttc ggcgccatcg
     5101 ggaccgccgg ccagcgctgc accaccattc gccgcctgtt cctccagaag ggcatcgcga
     5161 aagaaatgac ggaccggctc accgcggcct accgccaggt gcgcatcggc gatcccctgg
     5221 atgaatccgt gctgatggga ccgctggtaa accgtaagtc cgtcgaggaa atgcagcgtg
     5281 ggctcgatac gattcgctcc cagggcggcg agatcctctg cggcgggaaa gcgctggacc
     5341 gcccgggtgg atgcttcgtc gaaccggccc tcgtccgcgc ccggccggat atgcccgtgt
     5401 tgcgggacga gcttttcgct ccggtgctct acctgatcga gttcgacagc ctggaagagg
     5461 cggtccgctg gcacaatgac gtcccgcaag gcctctcctc ggcgatgttc accaccagtc
     5521 tgatgtcggc tgagatcttc ctcagcacgc gcggcagcga ttgcggcatc gccaacatca
     5581 acatcggcac cagcggcgcg gagatcggcg gggcctttgg cggcgagaaa gaaaccggcg
     5641 gcggccgcga ggccggcagc gacgcgtgga agcagtacat gcgccgccag acggtcacca
     5701 ttaactggtc caccaacctt ccgctcgccc aagggataaa atttgagcta tagaccgcag
     5761 ccataccggt cttctgggag gctccattgc cgatctacac ctgtgaagac atgatccggg
     5821 attgccgggc cgggaagaca gagggctggt cctacttcgt ccagcagtac gtacccgtcg
     5881 tccgccgcct tgcggatcac tactatcccg agcgcgccgg cgacaccgat ctctccgctc
     5941 gcgtgcttca gacgcttggc aagccggacc tgccgttgtt cgtggcggcc ggccctctct
     6001 ctgagcgcga tttcgtcgcc gatctgaggc aattcgtcct cggtgcaatc gaagaggaca
     6061 gtgccagcga cgaggccgaa attagcgtgg atgtggagac gctggcgtcc gcgctcgagt
     6121 cgctcagtac gatcgaaaag caagtggcct ggctggaagc aatgcgctac ggcgcggacg
     6181 agaccgcgca catgatggcc atgcacgctt ccacggtcgc taatattcgg gaacgggccg
     6241 ctgagttgct ccggtcgcgc gtgtcggcct ggcaggccgg catgattgcc ggcaacggtc
     6301 cggcgctgcg gcacgaagcc gtgctcctcg gaaaagacga ctgtccctcc gcacgcgtct
     6361 ggctcgactg gctcgacggc cgcatcacct ggtaccaacg cgaagacgcc gaacgccggc
     6421 tcgccgcctg ctggcactgc atagaccact tcagccgcct tcgcgaagcg cagtccctcc
     6481 tccgcgagct gaagccgctc accgccgccg aagccgaacc cttccgccgc gcatttggac
     6541 ttcagtccgt caaaccagcc ttctggaagc gctttggcag ccgggagtga cgctggttcg
     6601 cgcccctacg cccgcacctc gtccagccac tcgggacgtt tgagcacctc gcgcggcttt
     6661 gagccgtcgg gcggcccaat gatgccgtcg tgctgcatca tgtcgagaat gcgcgccgcc
     6721 cggccgtagc cgagccgcag ccggcgttgc agcgtcgagg tcgacgcctt ccccatttcc
     6781 agcaccacgc gcactgcgtc ctcgtagagc gggtcctgct cgccatcgag attctcgagg
     6841 tccgcctcgt cgtcctccga cggcggcgcc accaggaatg tcgcatcgta ttcgggccgc
     6901 gcctgctgct tccagaaatc caccacgcgg ttgatctccg tctccgtcac cagcgcgccg
     6961 tgtacgcgga tcagccgcga cgaacccggc ggcaggaaca gcatgtcgcc cttccccaac
     7021 agatgctcgg agcccatcac gtccagaatc gtccgcgagt ccactcgcgt cgctacccgg
     7081 aagctgatcc gcgccgggaa attcgccttg atcagccccg tga
//
LOCUS       DATIFQ010000239         6931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1227015, whole genome shotgun sequence.
ACCESSION   DATIFQ010000239 DATIFQ010000000
VERSION     DATIFQ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227015"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VLE22_11050"
     CDS             <1..934
                     /locus_tag="VLE22_11050"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB14987.1"
                     /translation="AVADTDEKRLSGDLSGIQGNIGGPGEKLDFSKLRRYSDWREAVK
                     DPEVEAVDICFPTSFHADVTVGALRAGKHVLVEKPMGLDGAAVDAMVAEAKRSGRILM
                     SAQVLRFFPAYRVMADMVKTGKLGPVRSAIFRRRCSAPAWSPWMADKKVSGGGVFDLL
                     IHDIDFCLHMFGKPESVSAVGYEDLPRGIDWITAELRYPSIGGVVISGGWHHPKAFPF
                     SMEFTVVTDGGTLEYHSSSTPLTLYRADGEQEALAVPEKDSYQAEIEYFIDCCAKGKQ
                     PDFCPPRESADSVKLALLILEAREKKGEKIACRL"
     gene            922..1764
                     /locus_tag="VLE22_11055"
     CDS             922..1764
                     /locus_tag="VLE22_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB14988.1"
                     /translation="MPALKDLEIGAMFWAGPDPVQTLKQVKSIGVRCGQVGVAGDVKL
                     DSAYTAAWKAALAAEDFTLVTAFAAYVGESYADGPTVHRTVGFIPPATRDERERRTYE
                     LCDFAAALGTPGIACHVGFVPEHTADPDYVAVRGMVQRICDYAAKYGQTFALETGQEP
                     ANVLLGFFKNVGRPNLRINFDPANLIMYGTGDPIEALDVLGPFVVSVHAKDGNWPDKN
                     VPGALGVEYPLGQGSVGMERFIAKLKAIGYKGTLNIEREVEDPGVKWRDMKDAVGLLR
                     KLVG"
     gene            1842..2777
                     /locus_tag="VLE22_11060"
     CDS             1842..2777
                     /locus_tag="VLE22_11060"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HSB14989.1"
                     /translation="MVPVILAVLGVVASLLQAQTVTPKKTTGPGVVVSERVEEQTTER
                     RIPGGSETVTRRSSERSEVQEIRRRRSAELPPAGTVRVPGAEAGTSDAPPVSPQTPVP
                     QPQPEFAIVPELVGLNREQAVAAIKEAHLTLGVERLVASDRQGGTVIAQRPRAGMRVP
                     FDAQVHLILAEAEAVTPPPAPPEEPSSPNEASGPPGPSDQPEQPANPEPSPRSYSLGP
                     TEPLRPVHSPQPPSVTVRLVGTGTAILVALLGGAYFWSRKYWKLPRSISFQPHPDPGS
                     SSVGSGLSFVVESEVRFRCIADLGRQRIEGKVVSA"
     gene            2774..3355
                     /locus_tag="VLE22_11065"
     CDS             2774..3355
                     /locus_tag="VLE22_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14990.1"
                     /translation="MTLREFFLSPGQDLAKGVARGIETGGDTVAVGIGGAADVRMLKW
                     PVFSSAVSRHIGELLDVGIPDVLANAWNKSAAVREHLEKSRQSPGESFLLPLAEHTIE
                     SEHTPSIEVLKNGKPVAKLAFTARVVLTVEGAVLRIQNGEIREIQTGQVKGSGTLKCG
                     GAVLVEKETQAIPLPGRVVLREDTSAAASASAT"
     gene            complement(3374..4429)
                     /locus_tag="VLE22_11070"
     CDS             complement(3374..4429)
                     /locus_tag="VLE22_11070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB14991.1"
                     /translation="MMVRFLTSTPLNAVKGSGTYAGIATLAGGLRALGVDVEVVAPDF
                     DFPIHTLHRLVFNEQLWWRNASGCDAVVGFDMDGYRVAGRRGVPHVASIKGVIADEMR
                     FERGTTRMTMSVQAACERRHVRKADLVMTTSRYTASRLEELYQIPKVGSIVPELIDLA
                     AWRMLFERNPGKPDPTRFTVFCVCRFYPRKRLHLLLRAAARLREGIPELEVRIAGGGP
                     EWPKLNALWRQERLENTVKWLGDVSQDQLAREYQQCDLFCLPSVQEGFGIVFLEAMAA
                     GKPIVAVRAAAVPEVVEQGVLVDPESGEALAEAILALYGDAAKRATLAEAGRSIVERY
                     DAPRVARLFLDEVHSLL"
     gene            4602..5330
                     /locus_tag="VLE22_11075"
     CDS             4602..5330
                     /locus_tag="VLE22_11075"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HSB14992.1"
                     /translation="MPWHFDPNKGTIKPRMGLRRNIPGDPIGAALFGATRQAVLGFFF
                     THIDERFYQRQVLRALRLGSGAVQRDLSRLCKCGILVRTVEGRQTYYQVNRQCPVVDE
                     LRGLIRKTFGVSYVLQTNLRALANRIQIAFVYGSLAEGRETPSSDIDVMIVGDHVTLD
                     EIVSAFSTAQAELAREINPSLYRIAEYCRKLSEGHHFLSSVMDGPKMFLIGDETILAG
                     LVQEWVAQRAQGKPAGDRRPPRRR"
     gene            5491..5688
                     /locus_tag="VLE22_11080"
     CDS             5491..5688
                     /locus_tag="VLE22_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14993.1"
                     /translation="MTLGLDVMTVRKLDTFRRKHNISDYERAHTISDLEAEEMIGLAV
                     RLRQEIRPWLAQNHPSLTPRR"
     gene            complement(5780..>6931)
                     /locus_tag="VLE22_11085"
     CDS             complement(5780..>6931)
                     /locus_tag="VLE22_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB14994.1"
                     /translation="LSHELPREKQWVTASFVVWKADAILVEVLTDKGIIGIGEPSPYG
                     GPEAVKKFIDEEIRPRLIGKNPFDVDHLTCTWGDSPFRYQVAWAGVDAALWDIIGKAK
                     GTPVYKLLAGDGQPEPHIRMYASGGVEYAWYKRPEDLIDEAVRHKGNGYSAFKFRIGT
                     EWKNSGMTIPKYIPYLRKMREAVGPGFDLIQESNMRWTLEECLEICPVLEELKFLWFE
                     EPVDRRAEGAIENYIKVNHALPTVKVSGGETMTTRFDFKQWVDRDAYEIVQPDVNTTG
                     LSEGWHIARVAALQGKPCCPHNWHGGLTTMANAALVAAIPNRLMLELNQTFNPLKEEV
                     FKDPLVVKKGYMDLPDKPGFGVELAANLAKKFPYVPGPYMKPNPDLPPV"
BASE COUNT         1280 a         2163 c         2129 g         1359 t
ORIGIN      
        1 cgcagtggcg gacacggacg aaaaacgcct aagcggtgat cttagtggaa tccagggcaa
       61 cattggcggg ccgggagaga agctcgactt ctcgaagctt cgccgctact ccgattggcg
      121 tgaggcggtg aaggacccgg aagtcgaggc agtcgatatc tgcttcccca ccagtttcca
      181 tgccgacgtg accgtcggtg cacttcgtgc cggaaagcac gtgctggtgg agaagccaat
      241 ggggctcgac ggtgcggctg tagacgcgat ggtcgccgag gcaaagcgca gcggtcgaat
      301 tctgatgagc gcacaggtgc tccgcttctt cccggcgtac cgggtgatgg cggacatggt
      361 gaagaccggt aagctcgggc cggtgcgttc cgcgattttt cggcggcggt gctcggcgcc
      421 ggcgtggagt ccatggatgg ctgacaagaa ggtgagcggc ggcggcgtct tcgacctgct
      481 gattcacgac atcgacttct gccttcatat gttcggcaag cccgagtccg tttcagcggt
      541 cggttacgaa gatctgccgc gcggcatcga ttggatcacg gccgagcttc gctatccgtc
      601 gatcggcggg gtggtcattt ccggcggatg gcaccacccg aaggcattcc cgttctcgat
      661 ggagttcacg gtcgtcaccg atggcggcac gctggaatac cactcctcga gcacgccgct
      721 gacgctttac cgcgcggacg gcgaacagga ggcgctggcg gttcctgaaa aggactcgta
      781 tcaggcggaa atcgagtact tcatcgactg ctgcgcgaag ggaaagcagc cggatttctg
      841 cccgccgcgc gaatcggcgg actcggtcaa actcgcactg ttgattctgg aagcacgaga
      901 gaaaaaggga gaaaaaatcg catgccggct ctaaaagatc tggaaatcgg cgccatgttc
      961 tgggcgggtc ccgacccggt tcagactcta aagcaggtga aatcgatcgg ggtgcgctgc
     1021 ggtcaggtgg gagttgccgg ggacgtgaaa ctggactccg cttacaccgc cgcgtggaaa
     1081 gccgcgcttg ccgcggagga tttcacgctg gtcacagctt tcgccgcgta cgtcggcgag
     1141 agttacgcgg acggaccgac agtgcatcgc acggtgggat tcatccctcc ggccacacgg
     1201 gacgagcgcg agcgccgcac gtacgagctc tgtgactttg ccgctgctct tggtacgcct
     1261 ggcatcgcct gccacgttgg tttcgttccc gagcatacgg ccgatccgga ctacgtggct
     1321 gtacgcggga tggtgcagcg gatctgcgac tacgcggcga agtacggcca gactttcgcg
     1381 ctcgagaccg gccaggagcc tgccaacgtt ctgctcggct tctttaagaa cgtcggccgc
     1441 ccgaaccttc ggatcaactt cgacccggcg aacctgatca tgtatggaac gggcgatccg
     1501 atcgaggctc tggatgttct cggccccttc gtcgtttccg tgcacgcgaa agacggcaac
     1561 tggcccgaca agaacgtgcc cggcgcactt ggcgtcgagt acccgcttgg ccaaggctcc
     1621 gtcggcatgg agcgcttcat cgcgaagctg aaggcgatcg gctataaggg tacgctcaac
     1681 atcgagcgcg aggtcgaaga tcccggtgtg aagtggcgcg atatgaaaga cgccgtgggt
     1741 cttcttcgga aactggtcgg ctgatcgtgg ccgccgccga ttttcgcttg taacggccgg
     1801 ctctttcgga tcactataag ctcagaaggg aggtggggac catggttccc gtcatcctcg
     1861 cagttctggg cgtggtcgca agtctgcttc aggcgcagac cgtcactcca aagaaaacga
     1921 ctggtcccgg ggtcgtcgtg tccgagcgcg tcgaagagca gactacggaa cggcgcatcc
     1981 ccggcgggtc cgaaacagtg acccgacgat ccagcgaaag aagcgaagtg caggaaatac
     2041 ggcggcgtcg ttcggcggaa ctaccgcctg ccggcacagt gagggttccc ggtgccgagg
     2101 caggcacttc cgacgcgccg ccggtcagtc cacaaacgcc tgttccgcaa cctcagcccg
     2161 agttcgccat cgtacccgaa cttgtcgggc tcaaccgtga gcaggctgtc gcagccatca
     2221 aggaagccca tctgaccctt ggcgtcgaac ggctcgttgc ctcggaccgt cagggcggaa
     2281 ccgtcatcgc gcagcgtccc cgggccggaa tgcgggtgcc ttttgacgcg caggtgcatc
     2341 tgatactagc cgaggccgag gcagtgacac caccgcccgc tcctcctgaa gagccgagtt
     2401 caccaaacga agcctcggga ccaccagggc cttcagatca gccggagcag ccagccaatc
     2461 ctgaaccttc tccaaggtcg tactcgttgg gtcctactga gcctttacgg ccagtacatt
     2521 cacctcaacc tccgagcgtc actgtccgcc tcgtcggaac cggcactgcc atcctggtcg
     2581 ccctgcttgg cggggcttac ttttggagcc ggaaatactg gaagctgccg cgatcgatca
     2641 gcttccagcc gcaccccgat cccggctcat cctcagtcgg ttccggactt tcgttcgtgg
     2701 ttgagtcgga ggtccgtttc cgttgcatcg ccgatctcgg gcggcagcgc atcgaaggta
     2761 aggtggtatc cgcatgactc tccgtgagtt cttcctctcg cccggccagg atctggcaaa
     2821 gggcgtcgcg cgagggattg aaacgggagg cgatacagtc gccgtcggga tcggaggcgc
     2881 ggccgacgtc cgcatgctga agtggccggt gttttcctcc gctgtctccc ggcacatcgg
     2941 cgagttactc gacgtcggta tcccggacgt tctcgcaaac gcgtggaaca agtccgccgc
     3001 agtgcgcgag cacttggaga agagccgcca gtcgccgggt gagagctttc ttcttccctt
     3061 ggctgaacac accatcgagt cggaacacac acccagcatc gaggtgctga agaacgggaa
     3121 gccggttgcc aagctcgcgt ttacggccag agtcgtcctg acagtcgagg gggcggtcct
     3181 ccgcattcag aacggcgaga ttcgcgagat ccaaacagga caggttaagg gcagcggtac
     3241 cctcaagtgc ggcggcgccg ttctggtcga gaaggagacc caagcgattc cgctgcccgg
     3301 acgagtcgtg ctcagagagg acacctccgc ggcggcctcg gcctctgcca cgtgagtgcg
     3361 ggtctggcgg ccttcaaagc agcgagtgca cttcgtcgag gaagagtcgc gctacgcgcg
     3421 gggcgtcgta ccgctcgacg attgaccggc cggcctccgc cagagtggct cgcttcgccg
     3481 catcgccata gagtgccagg atcgcttcgg ccaacgcctc gccgctctct gggtcgacca
     3541 gcacaccttg ctcgacgact tccggaaccg ctgccgcacg caccgctacg atcggtttcc
     3601 ccgccgccat tgcctccaga aacacgatgc cgaaaccctc ctgcacgctt ggaaggcaga
     3661 acaggtcgca ttgctggtac tcgcgcgcca gctggtcttg ggatacgtca ccgagccact
     3721 tcaccgtgtt ctcgagccgt tcttgccgcc acagagcatt gagtttcggc cactcgggcc
     3781 ctccgcccgc gatgcggact tccaactcgg ggatcccctc gcgcaaccgt gccgccgcgc
     3841 gaagcaacag gtgcagccgt ttgcgcggat agaagcggca cacgcaaaag acggtgaacc
     3901 tggtcgggtc cggtttgcca gggttgcgct cgaacagcat ccgccaggca gcgaggtcga
     3961 tcagttccgg tacgatgctt ccgaccttcg gtatctggta gagttcttcc agtcggctcg
     4021 cggtgtaccg gctcgtcgtc atcaccaggt ctgccttgcg gacgtgcctc cgctcgcagg
     4081 ccgcctgcac ggacatcgtc atcctcgtcg tcccgcgctc gaaccgcatc tcgtcggcga
     4141 tgacgccctt gatcgacgcc acatgcggga cgcccctgcg ccccgccacc cggtagccgt
     4201 ccatgtcgaa accgaccacg gcgtcgcagc cgctggcatt ccgccaccag agctgttcgt
     4261 tgaagaccag ccggtgaagg gtgtggattg gaaagtcgaa atccggcgcg acgacctcca
     4321 cgtcgactcc gagtgcccgg aggcccccgg ccagcgtggc gattccggcg taggttccgc
     4381 tgcccttcac ggcattcagg ggagtcgaag taaggaagcg gaccatcatc cggcttgata
     4441 ttatcgctta gcgtgaaatc ccgactgctg ctcgccttgt tgtctttgtc ccttcttccc
     4501 gccggcgaca ccgctgaact cacgctgcgc atcagcgttt acgccaccgc cggcggcgtg
     4561 aaccgatacc tccgcggcgc ctactgcccg acgcgttatc gttgccgtgg catttcgacc
     4621 ccaataaggg tacaataaaa cccagaatgg gtttaaggcg taatatcccc ggcgacccaa
     4681 ttggagcggc actcttcggg gctacccggc aggccgtact cggattcttc ttcacgcaca
     4741 tcgacgaaag gttctaccaa cggcaggtcc ttcgcgcact acggctcggt tcaggagcag
     4801 tacagcgaga tctcagcagg ctctgcaaat gcggcattct tgttcgaacc gtggaaggcc
     4861 gacagacgta ttatcaggtc aaccggcagt gtcccgttgt cgatgagctc cgcggcctca
     4921 tccgcaagac cttcggggtc tcgtatgttt tacagacaaa cctgcgtgcg ctcgcgaatc
     4981 gaattcaaat cgcctttgta tatggttcgc tggccgaggg ccgggagacg ccgtccagtg
     5041 acatcgatgt gatgatcgtc ggagaccatg tgacgctcga tgagatcgtg tcggcgttca
     5101 gtaccgcgca ggctgaattg gcgcgcgaga tcaatccctc actgtatcgg attgccgagt
     5161 actgtcgtaa actctcagag ggccaccatt tcctttcaag tgtaatggat ggcccgaaga
     5221 tgttcctgat cggcgatgaa actatccttg caggactggt gcaggaatgg gtggctcaga
     5281 gagcacaagg caagcccgca ggagatcgcc gacctcctcg acgtcgttga tcgtgacctt
     5341 gagtcttgcc ggacgcccgg gcttgtcgcc gactggcgct tcaacatcgc ctacaatgct
     5401 gcgcttcaac tcgcaactgc tgcccttgct gcatcgggat tccgggcaga gcgctcatcg
     5461 catcactatc gagtaatcga gtcgctttcc atgaccctcg ggctagacgt catgacggtt
     5521 agaaaactcg acacgttccg gaggaagcac aacatctcgg actacgagcg ggctcacacg
     5581 atctctgacc tcgaggcgga agagatgatc gggctcgccg tgcggctgcg gcaggagatc
     5641 cgcccctggc ttgcacagaa tcacccatct ctgacacctc gccgatgagt gccgtttgga
     5701 gtcggtgaca gggcactgat acgcgccacg gccgccttac tctggtggcc gccgctcagg
     5761 gctcagaatt ggctgaagct tacaccggcg gcaggtcagg gttcggtttc atgtacggac
     5821 ccgggacgta cgggaacttc ttggcgaggt tggcggccag ttcgacgccg aagccgggct
     5881 tgtcaggcag gtccatgtag cccttcttca ccacgagcgg atctttgaag acctcttcct
     5941 tgagcggatt gaacgtctgg ttcagctcaa gcatcaggcg attcggaatg gccgcaacca
     6001 aagctgcgtt ggccatcgtc gtcaaccctc cgtgccagtt gtgggggcag catggcttgc
     6061 cctgcaacgc ggccacccgt gcgatgtgcc atccttcgct gagcccggtg gtgttgacgt
     6121 ccggctgcac gatctcgtag gcatcgcggt cgacccactg cttgaaatcg aatcgtgtcg
     6181 tcatcgtttc gccgccggaa accttcaccg tcggaagcgc atgattgacc ttgatgtaat
     6241 tctcgatggc gccctcggcg cggcgatcga ccggttcctc gaaccagaga aacttcagtt
     6301 cttcgagcac agggcagatc tccaggcact cctcgagggt ccatcgcatg ttgctttcct
     6361 gaatcaagtc gaagcccggc ccgaccgcct cccgcatctt cctgaggtac gggatgtact
     6421 tcgggatcgt catgccgctg ttcttccact ccgtgccgat acggaacttg aaggcggaat
     6481 agccgttccc cttgtggcgc accgcttcgt cgatgagatc ttcggggcgc ttgtaccagg
     6541 cgtattcgac ccctccgctc gcgtacatcc ggatgtgggg ttccggctgg ccgtcgcctg
     6601 cgagcaattt gtacactggc gtgcccttcg ccttgccgat gatgtcccac agcgccgcgt
     6661 ccacccccgc ccacgccacc tggtagcgga acggagagtc gccccaggta cacgtgaggt
     6721 ggtcgacgtc aaaggggttc ttgccgatca ggcggggccg gatctcttcg tcgatgaact
     6781 ttttcaccgc ctccggaccg ccataggggc tcggctcgcc gatgccgatg atgcccttgt
     6841 cggtgagaac ctcgacgagg attgcgtcgg ccttccagac gacgaagctg gcggtcaccc
     6901 attgcttctc acgcgggagt tcatgggaga g
//
LOCUS       DATIFQ010000240        11078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2375879, whole genome shotgun sequence.
ACCESSION   DATIFQ010000240 DATIFQ010000000
VERSION     DATIFQ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2375879"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VLE22_11090"
     CDS             complement(<1..183)
                     /locus_tag="VLE22_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14995.1"
                     /translation="MNSAGIALVLALAACPRATLTQTAPVKEALRHGLSWHYRWIERL
                     PSGVAAIRCTIAYFEER"
     gene            complement(399..1178)
                     /locus_tag="VLE22_11095"
     CDS             complement(399..1178)
                     /locus_tag="VLE22_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB14996.1"
                     /translation="MAAVSWLAAQTSRPNDAALRRLERQLTAVLRWGILWGNLPAGFR
                     VTGDGSARLAIHRTPNLFSFCSPDLGTCAEYAMEGRKIGQRNRSVPCVSGKPPCSQGM
                     NDIESVLAFAGANRQAPAAQKTTERPLFGGTISGTLGSPGAIIWTATIKLDNRAETLK
                     YYQQLRPAELDGLREWLRTHLRGAGYKSVTIPCFASSDPKIYVYGDRPERGPIVFSVF
                     WDEESEEWVEAGRLDRMEDRAQIDKLKSLIEPIACSTIRFD"
     gene            1327..2001
                     /locus_tag="VLE22_11100"
     CDS             1327..2001
                     /locus_tag="VLE22_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HSB14997.1"
                     /translation="MRTDFVLFGAAHLAIIAAIPLLAGLLALPARRMPTAGRRIRLTL
                     GCFLLVNELVWYAWRIHTEGVRFPEDLPLQLCDLALWMTVLAALTLKQWSVEIAYFAG
                     LGGSSMAVLTPDLWAPLLSYPTIYFFLAHGGVIIVILFLVWGKLARPRAGCLWRVLAI
                     LNGYAAVIALFNFAFRTNYLYLCRKPASASLLDFMGPWPAYILVGELLAVAVFWLLWL
                     PFRRTA"
     gene            2134..5001
                     /gene="secA"
                     /locus_tag="VLE22_11105"
     CDS             2134..5001
                     /gene="secA"
                     /locus_tag="VLE22_11105"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSB14998.1"
                     /translation="MIDALIAKIFGTKHEREVKAIRPLVAAINELEPDLQQLDDLQLA
                     HKTAEFRERLAQGAALDDLLIEAFAVVREVGRRALNMRHFDVQLIGGVVLHRGKIAEM
                     KTGEGKTLVATLPVYLNALEGKGVHVVTVNDYLAKRDSEWMGRIYKFLGMSVGVIVHD
                     LDDEERRDAYNCDITYGTNNEFGFDYLRDNMKFRLEDCVQRGHHYAIVDEVDSILIDE
                     ARTPLIISGPSEESTDKYFRVNRIIPKLIRGEVIEGKEPGEKYTTGDYTVDEKHRSVA
                     LTEEGVAKAEKLLGVVNLYDPVNIELNHHVQQGLKAHVLFHRDKDYLVKDGEVIIVDE
                     FTGRVMPGRRWSDGLHQAVEAKEGVKIERENQTLATITFQNYFRMYKKLAGMTGTADT
                     EAAEFSKIYNLDVTVIPTNRALIRREFDDTVYRTEDEKWGNAAKEIKECNERGQPVLV
                     GTISVEKSEKLSGALKRMGVRHEVLNAKNHEREAFIVAQAGRKGAVTVSTNMAGRGTD
                     ILLGGNPEFMTKEACLKEKLAEMLPKDQASFIGDHEFYFFYHLGSFYRVQRSKWDEIF
                     ARYKAQTDAEHDDVVSVGGLHIVGTERHESRRIDNQLRGRAGRQGDPGSSRFYLSLQD
                     DLLRVFGGERIQGLMLRLGMEENVPIESRLITKRIAKAQEAVEAQNFAARKHLLEYDD
                     VMNKQRQAVYGLRRQLLEGKEQKDRILEMVKGIVESFVDTRCPDNVHPKEWDLVSLRT
                     DVLSQFGLKIDVQELSEMRAAEIGETIYQRLVAKYEEKEQMVGKEIMRETERILLLQV
                     IDDQWKDHLLSMDHLKEGIGLRGYGQKDPLVEYKKESFLIFQEMMDRIEDEAVRYLYF
                     LRPVEEVRPIAADVQEEEFEEEPEALASVAQVQRSAAQSSIQDFTRNIQRKKEKEMAE
                     LQFVGGGSPSGQQPVVKAQKVGRNDPCPCGSGKKYKKCCGA"
     gene            4986..5963
                     /locus_tag="VLE22_11110"
     CDS             4986..5963
                     /locus_tag="VLE22_11110"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HSB14999.1"
                     /translation="MLRSITLILLAAGTLAQAAIWPEEFAGLKRTSVKPVAVQPSPVW
                     EEYGFEEAEEAAYTASDGKFTATAWRLGDSTGALAAFQWQRAAAAQPSDLGKLAVETP
                     GSVLLAFGNYLFRFDGRRPEIRELAHLFDVLPRLEQSSLPALSDYLPAGNLAPNSERY
                     VLGPVTLAAFEPRIPPAVAAFRYGTEAQLGKFRTKAGEMSLVVFSYPTPQIARERIVE
                     FDKLPGALAKRSGPIIAVIVSPPDANEAEKLLSQIRYKASLTWSEYTADKRENVADLI
                     LTALTLTGIILVFCLASSLAFAGFRLLTRKLFGHSDAGDPMTLLHLQDR"
     gene            6317..7984
                     /locus_tag="VLE22_11115"
     CDS             6317..7984
                     /locus_tag="VLE22_11115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HSB15000.1"
                     /translation="MADGKLQVIPLGGLGEFGMNSMALRYGDDILVIDAGMMFPEDEL
                     LGVDIVTPDFAYLEENREHVRALVLTHGHEDHIGATPFLLSELDIPVYGTAFTLALVE
                     RRLEEHDLAGKVKLNVIKGGEKVQIGPFLVDFVHVTHSIVSALALAITTPLGVVIHTG
                     DFKVDPTPTDNLLFDLHTLAEYGKRGVLLLLSDSTNVDRPGYTESERAVRPRFEEIFN
                     RAERRVVIACFSSSVHRLQQILDMADEAGRKVCFLGRSMTSVTEIAHSLGLLQIPDGI
                     LLRPQDVMQARPDKVCVVVSGTQGEPMSAMSRVAVDNHKHLSIGQGDTVVLSSRIIPG
                     NEKAIYRMINHVSRRGADVIYGSMNPPVHVSGHASSEELKLILNLVRPRFFMPIHGEY
                     RQLAKHAGLAQHLRHAGLEDTFIVETGETLELDRHGARKGGRVPVGRICIDSGTLDEV
                     VEDMVIRDRRHLSEDGFVLPIITINKLTGMSEGLPEIVSRGFISEDGSEILVDARKVV
                     ARTIETSSVEERTDWGVMKEKIRTDLRRFLIKETSRRPLIMPVILEV"
     gene            7986..9350
                     /locus_tag="VLE22_11120"
     CDS             7986..9350
                     /locus_tag="VLE22_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSB15001.1"
                     /translation="MDIPPDYLQDASGFRGFAERLFVPASEEKLVEVVREAARSATPL
                     TIAGGGTGITGGRVARGGWVVSLEKLNGLEIRTGSALAGAGVTLADLHAAAERNGQFY
                     PPDPTETSAFLGGTIATNASGSRSFRHGDTRRHVLALRVVLADGRTLAVRRGELVDFP
                     IPALPLPRTTKHSAGFRLAPKMDWIDLFVGSEGTLGVIVEAEVRLLPAPKELLAGVVF
                     FPGDAGALDAVDAWRSCAGLRMIEYFDAGSLALLRERFPDTPAAARAALLIEQELASP
                     DDDEMDRWVDRLEECGALADDSWFAVGAADRERFRRFRHALPELVNDTVRRRGFLKVN
                     SDYAVPVERNREMLGYYRERLESGFAGRYVIFGHIGDAHVHVNILPASEEEFHRAGDL
                     MLEFARKAVSMGGTVSAEHGLGKRKAHLLAIQYTPEEIEAMKDVKRRLDPHWLLGRGN
                     LFGE"
     gene            10038..>11078
                     /locus_tag="VLE22_11125"
     CDS             10038..>11078
                     /locus_tag="VLE22_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB15002.1"
                     /translation="MLDLLNDVAARAARYSGDIGRRPVAPPQSALDGLAELDTPLPVG
                     PSSPASVLDLLDRAGSPATVATSGGRFFGFVHGGCLPAALAANWLAGAWDQNAVLYVT
                     SPVGSAVEEIALRWLLEVLRLPPECGCAFVTGATTASFSALAAARNAVLEQAGWDVES
                     DGLTGAPQVTVVVSEEVHPSVTKALGLLGLGRRKVCHVPVDRQGRMRVDALPELRPPA
                     IVCVQAGNVNTGAFDPVGEICAIAHRSGAWVHVDGAFGLWALASPALAHLADGVQDAD
                     SWATDAHKWLNVPYDSGLAFVRDAARLNRAMALTASYIPLAARRDPGQFTPELSRRAR
                     GVEIWAALRSLGR"
BASE COUNT         2065 a         3477 c         3475 g         2061 t
ORIGIN      
        1 gcgctcctca aagtacgcga tagtgcatcg gatcgcagcg acaccagaag gcagtcgctc
       61 gatccagcga tagtgccaac tcaagccgtg tcgcagcgcc tctttcacgg gcgcggtttg
      121 cgtcagcgtg gcccgtggac aagccgcaag cgcgagaacg agtgcgatgc cggcgctatt
      181 catctgcggc gccccccagc cctccccggt cgtctaatcc ggtctattgc ttgcctccta
      241 tttatgggaa cgggtatagg tagtctcacg ccagagaacg tattgcggct tccgaaggtt
      301 ccgatccgtt cttcccaatc gtacttctgc tcgttgtttc gttgtaaatg cgtctgtaaa
      361 tacatcctaa atgcgtcagc ctgatgtggc acttttttct agtcgaacct aatcgtggag
      421 cacgctatgg gctcgatcag acttttgagc ttgtcaattt gagcccgatc ctccatccga
      481 tccagtcttc ccgcttccac ccattcctcg ctttcctcgt cccagaagac tgaaaatacg
      541 atcggccctc gttcgggccg gtcgccgtat acgtatattt taggatccga acttgcgaag
      601 catgggatcg tgaccgattt atatcccgcg ccgcggaggt gagtcctgag ccactcgcgc
      661 agcccgtcca gttccgccgg gcgcagttgc tggtagtact tcagcgtctc cgctcggtta
      721 tctaacttta tcgtcgccgt ccaaatgatc gcaccggggc ttcctaaagt gcctgaaatc
      781 gtgccaccga acaatggacg ctcagtcgtc ttttgagcag ccggcgcctg gcggttcgcg
      841 ccggcgaacg caagtaccga ttcaatatcg ttcattcctt ggctgcatgg cggcttgccg
      901 ctaacgcatg ggacgcttct gtttcgctgg ccgattttgc gtccctccat agcgtattcg
      961 gcgcaggtgc cgagatccgg gctgcagaag gaaaaaagat tcggtgtgcg gtggattgcc
     1021 agccgtgctg atccatcacc tgtgactcga aacccggctg gcagattgcc ccacaggatc
     1081 ccccacctca ggacggcggt cagttggcgc tcaagacggc gcaacgcggc gtcgttcggg
     1141 cgggaagtct gggctgcaag ccaggagacg gctgccactg cacaaagaat tctagaaaac
     1201 atgacgacag gtcttcagaa acaaaagcat cttcgaagtt ccgagtcgtg tcaaggccta
     1261 acttcgccgg aaaccgtttg ctttccagag aaccggtgga gtcaccccgg cgctaagctg
     1321 agttcaatgc gcacggactt cgtgctcttc ggcgccgccc acctcgcaat catcgccgcg
     1381 attcccctcc tcgcgggact cctcgcactt ccggcgcgcc ggatgcccac cgcgggccgc
     1441 cggatccgcc tcaccctggg ctgctttctc ctggtgaacg agctcgtctg gtatgcctgg
     1501 cgcatccaca ccgagggcgt ccgttttccg gaagacctgc ccctccagct ttgcgatctt
     1561 gcgctctgga tgaccgtgct cgccgcgctc acgttgaagc agtggagcgt cgaaatcgcg
     1621 tacttcgccg gactcggcgg cagcagcatg gccgtgctca ccccggatct ctgggcgccg
     1681 ctcctctcct acccgaccat ctacttcttc ctcgctcacg gcggcgtcat cattgtgatt
     1741 ctcttcctcg tctggggaaa gctcgcacgc ccgcgtgcgg ggtgcctgtg gcgcgtcctg
     1801 gccatcctga acggctacgc cgctgtcatc gctctgttca acttcgcctt tcgcacgaac
     1861 tatctttacc tctgccgcaa acccgccagc gcctcgctgc ttgacttcat gggcccatgg
     1921 cctgcctaca tcctggtggg tgagctcctc gcggtcgccg tgttctggct gctttggctg
     1981 ccgttccgcc ggacagcctg aacagggacg caggacagga gtcctacgct acgctgtgga
     2041 tcaacgattt ccgctgaagt agggtaggtc tcccgacctg ccaaccagtt tcttcagcac
     2101 cctgtgaagt acaatgaaag taatcccact cctatgatcg atgccctgat cgccaagatt
     2161 ttcggcacga agcatgaacg cgaagtcaag gcgatacgcc cgctcgtcgc agccatcaac
     2221 gagctggagc cggatctcca gcagcttgac gatctccagc tcgctcacaa gaccgccgaa
     2281 ttcagggaaa ggttggcgca gggagccgct ctcgatgact tgctcatcga agccttcgcg
     2341 gtcgtgcgcg aagtcggccg gcgcgccctg aacatgcgcc atttcgatgt gcagttgatc
     2401 ggcggcgtcg ttctgcaccg cggcaagatc gccgaaatga agaccggcga aggcaaaacg
     2461 ctcgtcgcaa cacttcccgt ctatctgaac gcgcttgagg gcaagggcgt gcacgtcgtc
     2521 accgtcaacg actacctcgc caagcgcgac tcggagtgga tgggcaggat ctacaagttc
     2581 ctcggcatgt cggtcggcgt catcgtgcat gacctcgatg acgaggagcg ccgcgacgca
     2641 tacaactgcg acatcaccta cggcaccaac aacgagttcg gcttcgacta tctgcgggac
     2701 aacatgaagt tccgcctgga agactgcgtc cagcgcggcc atcactacgc catcgtcgac
     2761 gaggtcgact cgatcctcat cgacgaggcc cggacgccgc tcatcatctc cggccccagc
     2821 gaggaatcca ccgacaagta cttccgcgtc aaccgcatca tcccgaaact cattcgcggc
     2881 gaggtgatcg agggcaagga acccggcgag aagtacacca ccggagacta taccgtcgac
     2941 gagaagcacc gctccgtggc gctcaccgaa gagggagtgg ccaaggccga gaaactcctc
     3001 ggagtcgtca acctgtacga ccccgtgaac atcgagctga accatcacgt ccagcagggg
     3061 ctgaaggccc acgttctctt ccaccgcgac aaggactacc tcgtcaagga cggagaagtg
     3121 atcatcgtcg acgaattcac cggccgcgtc atgccgggcc ggcgctggag cgacggactc
     3181 caccaggcgg tcgaggccaa ggagggtgtc aagatcgagc gcgagaacca gacgctcgcc
     3241 accatcacct tccagaacta cttccgaatg tacaagaagc tcgccggcat gaccggtacg
     3301 gcggataccg aagcggccga gttcagcaag atctacaacc ttgacgtcac cgtcatcccc
     3361 accaaccgcg cgttgatccg ccgcgagttc gacgacaccg tctaccgcac cgaagacgag
     3421 aagtggggca atgccgccaa ggaaatcaag gaatgcaacg agcgcggcca gccggtgctg
     3481 gtcggcacaa tttcggtcga gaaatccgag aagctctcgg gagccctgaa gcggatgggc
     3541 gtcaggcacg aagtcctgaa cgcgaaaaac cacgagcgtg aagccttcat cgtcgcccag
     3601 gccggccgca agggcgccgt taccgtctcc accaacatgg ccggccgcgg aaccgatatc
     3661 ctgctcggcg gcaacccgga gttcatgacc aaggaggctt gcctcaaaga gaagctggcc
     3721 gaaatgctgc cgaaggacca ggcgtccttc atcggcgacc acgagttcta cttcttctac
     3781 cacctcggga gtttctaccg ggtccagcgt tcgaagtggg acgaaatctt cgcccgctac
     3841 aaagcccaga ctgacgcgga gcacgacgac gtggtcagcg tcggaggcct ccacatcgtc
     3901 ggcactgaac gccatgagtc gcgccgtatc gacaaccagt tgcgaggccg agccggccgc
     3961 cagggcgacc cgggcagctc acggttttac ctctcgctac aggacgacct gctgcgcgtc
     4021 ttcggaggcg agcgcattca gggcctgatg ctgcggctcg gcatggaaga gaacgtgccg
     4081 atcgagtctc ggctcatcac caagcgcatc gccaaggccc aggaggccgt cgaggcgcag
     4141 aactttgccg cccgcaaaca cctgctcgaa tacgacgacg tcatgaacaa gcagcgccag
     4201 gctgtgtacg gcctgcgccg ccagttgctg gagggtaagg agcagaagga ccgtatcctc
     4261 gaaatggtca agggcatcgt cgagagcttc gtcgacacgc gttgcccaga caacgtgcac
     4321 ccgaaggagt gggacctcgt gtcgctccgg accgacgttc tgagccagtt cgggctgaaa
     4381 atcgacgtgc aggaactctc cgagatgagg gccgccgaaa tcggcgagac catctaccag
     4441 cgccttgtgg ccaagtacga ggagaaggag caaatggtcg gcaaggagat catgcgcgag
     4501 accgagcgca tcctccttct tcaggtgatc gacgaccagt ggaaagacca cctgctctcg
     4561 atggaccacc tcaaggaagg catcggactt cgcggctacg gacagaagga cccgctcgtc
     4621 gaatacaaga aggagtcgtt cctcatcttc caggaaatga tggaccgcat cgaggacgag
     4681 gcggtccgct atctgtactt cctccggccc gtcgaagagg tccgtccgat agcggccgac
     4741 gtccaggaag aggaattcga ggaggaaccc gaggcgcttg cgagcgtcgc acaagtacag
     4801 cgctccgccg cccagtcttc aatccaggac ttcacgcgca acatccagcg caagaaggaa
     4861 aaggagatgg ccgagttgca gttcgtgggt gggggatcgc cttccgggca acagcccgtc
     4921 gtcaaggcgc agaaagtcgg ccgcaacgat ccttgcccgt gcggcagcgg gaagaaatac
     4981 aagaaatgct gcggagcata accctgattc ttctcgccgc cggtactctg gcccaggcgg
     5041 cgatctggcc cgaggaattc gcgggcttga agcgcacgtc cgtcaagccg gtcgcggtcc
     5101 agccgtcgcc ggtctgggag gagtacggct tcgaagaggc cgaggaggcc gcttacaccg
     5161 ccagcgacgg caagttcact gccaccgcat ggcggctcgg agactcaacc ggcgccctgg
     5221 ccgcattcca gtggcagcgg gccgcggccg cgcagccgtc cgacctcggg aagctcgccg
     5281 ttgagacgcc cggctcagtt ctcctcgcgt tcggcaacta tcttttccgt ttcgacggac
     5341 ggcgtcccga aatccgtgag cttgcccacc tcttcgatgt cctcccgcgc ctcgagcagt
     5401 cgtctctgcc tgcgctgagc gactatctgc cggcggggaa tctggccccg aattccgagc
     5461 ggtacgtgct cggaccggtg acgctggcag cgttcgaacc gcgcatcccg cccgccgtcg
     5521 cggcgtttcg ttacggaacc gaagcgcagc tcggcaaatt ccggaccaaa gccggtgaga
     5581 tgtcgcttgt ggtcttctcg tacccgacac cgcaaatcgc gcgggaacgg atcgtcgaat
     5641 tcgacaagct tcccggcgcc ctggcaaaac gcagcggccc gattatcgcc gtgatcgttt
     5701 cgccgccgga cgccaatgaa gccgaaaaac tgctctcgca gatccgttac aaggcatcgc
     5761 tgacgtggag cgaatacacc gccgacaaac gcgagaacgt cgccgacctc attctcaccg
     5821 cgctgacgct cactggaatc atcctggtat tctgcctggc atcctcgctc gcgttcgctg
     5881 gtttccgcct tctcacgaga aaactgtttg gccactcaga tgctggagat cccatgacct
     5941 tgctccattt gcaggatcga taagcctctt tttttcaaat tcttagagtg aaataggacc
     6001 accccccgag ggcatttcca agcgtttctc atcacctacc gaaatagtac cctcctgtct
     6061 aagctcttat gacagtgcgg cttggacgcc tgcttttcgc gtgggctttt tctcttgact
     6121 tgcacccccg caggaaatat catccaaatc acaaaggttc tgacggttgc gtttccgtcg
     6181 gccttgattc tcccattgaa catcgaatgc tcacccaccg gttgggtgag catttccatt
     6241 ttcgggcccc ctctgccggt tccctgactg cttgaaaata aaggcgcact cgcggctgtg
     6301 ctacgctggt tctttaatgg ccgacggcaa gctgcaggtg atcccgctgg gcgggttggg
     6361 cgaattcgga atgaattcga tggccctccg ctacggcgat gacatcctcg tgatcgatgc
     6421 ggggatgatg tttcccgaag acgaactcct gggagtcgat atcgtcaccc cggacttcgc
     6481 gtatctagaa gaaaacagag aacatgtccg cgccttggtt ctgacgcacg gacacgaaga
     6541 ccacatcggc gcgacgccgt ttctcctttc ggagctcgac atcccggttt atgggacggc
     6601 cttcacgctc gcgctcgttg agcggcgcct cgaagagcac gacctcgccg gcaaggtaaa
     6661 actaaacgtc ataaaagggg gcgaaaaggt acaaattggg ccgtttttgg tcgattttgt
     6721 gcacgttacg cattcgattg tgagcgccct ggcgctggcg atcacaaccc ccctgggggt
     6781 cgtgattcac acgggcgatt tcaaggtaga cccaaccccg acggacaacc tcctgttcga
     6841 tctccacacc ctcgccgagt atggcaagcg cggcgtcctg ctcctgctct ccgactcgac
     6901 caacgtcgac cgcccggggt acaccgaaag cgagcgggcg gtccggccgc gcttcgaaga
     6961 gatcttcaac cgcgcggagc gccgcgtcgt gatcgcctgc ttcagctcgt ccgtccatcg
     7021 cctccagcag attctggaca tggcggacga ggctggccgc aaggtctgtt tccttggacg
     7081 cagcatgacg tcggtaaccg agatcgcgca ttcgctgggg ctgctgcaaa tccccgacgg
     7141 aattctgctg cggccgcagg acgtgatgca ggcgcgcccc gacaaggtct gcgtggtggt
     7201 gtccggcacg cagggcgagc cgatgtcggc catgtcgcgg gtggcggtgg acaaccacaa
     7261 gcacctttcg atcgggcagg gcgacacggt cgtgctaagc tcgcgcatca ttccgggcaa
     7321 cgagaaggcg atctaccgca tgatcaacca cgtctcgcgc cgcggcgcgg acgtgatcta
     7381 cggctccatg aacccgccgg tgcatgtttc ggggcacgcc agctccgagg aattgaagct
     7441 gattctgaat ctcgtgcgcc cacgcttttt catgccgatt cacggcgagt accgccagct
     7501 tgccaaacac gccgggctgg cgcagcacct tcgccacgcc gggctcgaag acacgttcat
     7561 tgtggagacg ggcgagacgc tggaactgga ccgccacggc gcccggaagg gcgggcgcgt
     7621 gcccgtcggc cgcatctgca tcgacagcgg cacgctggac gaggtggtcg aggacatggt
     7681 gatccgggac cgccggcacc tttccgagga cggcttcgtg ctgccgatca tcaccatcaa
     7741 caagctcacc ggcatgagcg aaggactgcc ggagatcgtc agccggggtt tcatctccga
     7801 ggacggctcc gagattctgg tggacgcgcg gaaggtggtg gcgcggacga ttgagacgtc
     7861 gagcgtcgag gagcggacgg actggggcgt catgaaggaa aagatccgca ccgatctgcg
     7921 ccgattcctg atcaaggaga cctcccgccg gccgttgatc atgccggtga tcctggaggt
     7981 ctaggttgga cattcccccg gactatcttc aggacgcctc cggcttccgc gggttcgcgg
     8041 agcggctgtt cgtgccggcg tccgaggaga aactcgtcga ggtcgtgcgc gaagcggccc
     8101 gctcggcgac gccgctcacg atcgccggag gaggcacggg aatcaccggg ggccgcgtgg
     8161 cccggggcgg ctgggtggtt tcgctcgaga agctgaacgg gctcgaaatc cggacaggca
     8221 gcgcgctggc cggggccggt gttacgctcg cggaccttca cgccgcggcc gagcgaaacg
     8281 ggcagttcta tccgccggat ccgaccgaga cctccgcgtt cctcggcggc accatcgcga
     8341 cgaacgcgag cggctcgcgg agtttccgtc acggcgacac tcggcggcac gtgctcgcgc
     8401 tgcgcgtggt gctggcggac gggcggacgc ttgccgtgcg gcgcggcgag ctggtggatt
     8461 tcccaatccc cgccctgccg cttccgcgga cgacgaagca ttcggccggg ttccgcctcg
     8521 ccccgaagat ggactggatc gacctgttcg tgggatcgga gggcactctc ggcgtcattg
     8581 ttgaggcgga agtgcgtctg ctgccggcgc cgaaggaact gctcgcggga gtcgtgtttt
     8641 tcccgggtga cgcgggcgcg ctcgacgcgg tggacgcgtg gcgaagctgc gcggggctgc
     8701 ggatgatcga gtacttcgat gcaggatcgc ttgcgttgct gcgcgagcgt tttccggata
     8761 cgccagcggc ggctcgggcg gcgctgctga ttgagcagga gctcgcctcg ccggacgacg
     8821 atgagatgga tcgttgggtg gaccggctgg aggagtgcgg tgcgctggcg gacgattcct
     8881 ggttcgcggt gggggcggcc gaccgcgagc ggttccggcg cttccggcat gcgctgccgg
     8941 agttagtgaa cgacacggtg cggcggcgcg ggtttctgaa ggtgaactcg gattatgcgg
     9001 tgccggtgga gcgcaaccgc gagatgctcg gttattaccg ggaacggctg gagagcgggt
     9061 ttgcgggacg gtacgtgatt tttgggcaca tcggcgacgc gcacgttcac gtaaacatcc
     9121 tgccggcgag cgaggaggag ttccaccgcg cgggcgacct gatgctcgaa tttgcgcgga
     9181 aggccgtctc gatgggcggc acggtatccg ctgagcacgg cctgggcaag cggaaagcgc
     9241 acctgcttgc gattcagtac acgccagagg agatcgaggc gatgaaggac gtgaagcgcc
     9301 gcctcgatcc ccactggctc ctgggccgcg gcaacctgtt cggggagtga gggggagaga
     9361 agaggccgga gtgcggcccg tggccaactt ccgaagccca gattcggcgg ctggtataaa
     9421 acgcgggtct tcgctgccct cgaggattga ctttctgcta actgacgtca ttaagaccac
     9481 gataccggcg gggatactga aagtgacgat cgaaggggct gttcgtacgt cggcgtgctt
     9541 tctccagttc agcggggact cggcgaccag cctgcacgcg gcgcatgacc tcgtgccggc
     9601 cgctcccagc cgcaccgcaa cggtccggta ggccaatcca caatccggga aaatccgttc
     9661 tgatatgcgg cgtgtggcga actggaacag attcagttca tcttcgggca cgtctcaatc
     9721 caaacgaccg agcgatacct cggccaagca gaacattcga tcctccgtta acgatcggat
     9781 cggcaccgat ccggaggact gagtcggtcg gttgcacgtc catggcttgc gaacaccgga
     9841 ccgagcgcgt cgaatgcctc tttgctcagc atgccagcgg cacttccgtg gttatagacg
     9901 acggcgggaa atggtcctgt tccttgtgat agcggagagc ggccttccga cagacaggtc
     9961 gtgagtggtc ggcaagggtt tagcagaccc ctgcgaaacg tttgcccgtc ttgcgccggt
    10021 gcgagtagtc tggtggaatg ctcgatctgc tcaatgacgt cgcggcgcgc gcggcacggt
    10081 actcgggtga cattggccgc aggccggtag ctccgccaca gagcgcgctg gacggtctcg
    10141 ccgaactcga cacaccgctt ccggtgggtc ccagcagccc ggcatccgtc ctcgacttgc
    10201 tggaccgcgc tgggtcgccg gcgaccgtcg ccacgagcgg cggccgattc ttcggcttcg
    10261 tgcatggcgg gtgtcttccc gcggcgctgg ccgcgaactg gctcgccggc gcgtgggacc
    10321 agaacgcggt tctgtacgtg acgagcccgg tcgggtcggc ggttgaggag atcgcgctgc
    10381 gctggcttct cgaagtcctg cggctgccgc cggagtgcgg gtgcgccttc gtcaccggcg
    10441 cgaccacggc cagcttcagc gctctcgccg ccgcgcgcaa cgccgtgctg gagcaggcgg
    10501 gatgggacgt ggaatcggac gggctgacgg gcgctcctca ggtgacggtt gtggtcagcg
    10561 aggaagttca cccgtccgtc accaaggcgt tagggctgct cgggctcggc cggcggaaag
    10621 tctgccacgt tcccgtggat cgtcagggca ggatgcgcgt ggacgcgctt ccggagctgc
    10681 ggccgccggc cattgtctgc gtgcaggccg gcaacgtgaa cacgggcgcg ttcgacccgg
    10741 tcggagaaat ttgcgcgatt gcccaccgga gcggagcttg ggtgcacgtg gacggcgcgt
    10801 tcgggttgtg ggcgttggcg agcccggccc tcgctcatct ggcggatggg gttcaggacg
    10861 ccgactcctg ggccaccgat gcgcacaagt ggctgaacgt gccgtacgac agcggtctgg
    10921 cgttcgtgcg ggacgccgcg cgcttgaatc gggcgatggc actgacggcc tcctacattc
    10981 cgctggcggc gcgccgtgat ccgggccagt tcacgccgga gctctcgcgg cgggctcgcg
    11041 gggtggagat atgggcggcg ctgcggtcgc tcggccgg
//
LOCUS       DATIFQ010000241        11350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_461364, whole genome shotgun sequence.
ACCESSION   DATIFQ010000241 DATIFQ010000000
VERSION     DATIFQ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11350
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461364"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1956
                     /locus_tag="VLE22_11130"
     CDS             <1..1956
                     /locus_tag="VLE22_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HSB15003.1"
                     /translation="LAIGTTITNTGVVRWNDPAQTASASVSIDVGGTPDSASLNGNVW
                     HDANLNKLRECNSENCALTGWTVELYRNSQLVATVLTDASGAYRLSGLAPNAGTSDLY
                     ELRFRAPGAGPNTASMGQADSPFTNGPQRISGITVAPGANLQNLNLPLWPNGTVYNSV
                     ARVPVAGARMTMRNAATGAVLPVQCFEDPAQQSQITAANGFYKFDLNFSDASCPAGGG
                     YLIEVTPPATGYLNGPSRIVAPSSDAQTAAFSVPACPGSAADAVPATPEYCEVVASPA
                     VPPLSVPPGTAGTVYHLHLLLSNGTVPGQSQVFNNQIAIDPELFGAVAISKTSSLTNV
                     SKGTLVPYTITVTNVYGAPLSGIGIVDRFPAGFKYVAGSARLDGNPAEPLINGRELAW
                     NGLELQFNQRRTIRFLLVVGSGVSEDEYVNRALVRNATTGAAISGEATATVRVIPDPD
                     FDCTDVIGKVFDDANLNGEQDSGEKGLFGVRVVTARGLIASTDNNGRFHITCAAVPDE
                     DRGSNFILKLDERSLPSGYRLTTENPRVQRATRGKMLRFRFGAAVHRVVRLDIADGAF
                     EPDTTKIRMQWTHRIDQLLEELRKKPSVLRLSYLGDVEPKGKALERLEALNKEITARW
                     KQSGAGYPLTIETEIFWRRGAPIAGHR"
     gene            1940..7084
                     /locus_tag="VLE22_11135"
     CDS             1940..7084
                     /locus_tag="VLE22_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB15004.1"
                     /translation="MPVTDRGLMKSMGRHLRIVKGFLLRHGIAVLCVCGVTAAAPGVA
                     SSEETGVRETHVRGEAAERHLPADRPLIPWVHDPAVFEKDEGDRKELREVDEKEVTTI
                     KLDNLVPPIHFGLGQIEITEDYLKMLRDVLDGMRGRANVRLHFIGHADTLPLRGELIH
                     IYGDNIGLSRERAGTVAEYCQRALNLPPEAISYEGLGDSRPVASNATEEGRQLNRRVE
                     VQVWYDEIGHKQVMKEVVVPRERNRIKVCRTETVCKLRYKDGHARRARIKNLTSPLHY
                     DKGMLSVPEEFLQQVRRARKNLGDKRNLVVKLTGYTDNIPLKGRDERIYGDPVGLSKA
                     VARRVALAVQEDLDLPSDAIDTEGRGASQPVAPNDTEQGRALNRRVEVEFWHDDPLQD
                     LPEEPQLCPDAPGAETVTRVYDSPSGGIDPILFENGKPVIPDGYTGHLRRIMDEIHDR
                     THVRLRFVGYTANKLLDRRTAAVYGDDIGLSMARARRSMAAVRTQMGLSERQAEFDGR
                     GYVQSDDVVNAGFIESETSRVKVQVVYDELIPRDDYEGVEVTPLIREVKTVDPFALNL
                     MRITVDGKPVDDPSKCSSDVQRCIDVALDNAQIQFKHDSLTLTPRLNVTAWPTTIRYQ
                     DAPDTAFAENLVHFRLYLNYRSFIERAEVRIFEEQQSVRDTPLAVVEMDADGRAQWQA
                     EFESFSAPVRKLKYLVRVVDRKGLFDETTPQPLWVADRIDPSVAEAKVDEKLLAGYGE
                     SRIASWNIRLHGGTVQAYGTAIPKGRGVWMAGYSVPVDEKGGFVAEEILPEGMHTVEV
                     AVLDKSGNGEIFLRDLELKKSDWFTVGIADLTLSGNKTDGPAELLAPDKPRYRDDMSL
                     EGRLAFYTHGKLENGWWFTASADTREGPLDEIFSNFLDKSPEALFRRIDPDYHYPTFG
                     DDGTVLEDAPTTGKFFVKAKKDNTYGLWGNFKVAYTDTQLAHVDRGLYGANLHYQPLG
                     TTSFGEPRLLVDGFAADPGTVAGRDEFRGTDGSLYFLRRQDVLEGSERVRIEVRDKDS
                     GIVLAVKNLTPVLDYDIDYLQGRILLSQPLPATADDGLLVHSASISGHPVFLVVRYEF
                     TPGVDEPDTLAVGGRAHYWFNDHVKVGVTASHDEEADIENSLGGADLTLRKSSASWLK
                     LEAGRTKGPGVPATTSIDGGFGFDNTAGFPVGNEVDAMAYRVDASLGFKDFFKKGRGK
                     VTFYWQDLEAGYSAPGQATARDLTQYGGTAEIPFTDRLSARAKMDKREEQEGLQTETA
                     ELNVDYRMGKHWTLGSGVRHDRRKDDSPAVPATQEEGDRTDAAVRLLYDSLKRWKTYG
                     FVQGTVQSSGTQEENDRIGAGGSVRVTDRVNVTGEVSGGDLGTGGKLVTEYLYTDRTT
                     LYGNYSLENERTDNGLLARKGKMASGFRTRYSDSASVYLEERYTHGDVPTGLTHAAGV
                     KLVPFDRLNFGANIDFGTLKDPRTAAELKRTALGVSAGYGFARLALATAVEYRVDDIE
                     QPDTSFSKRTSWLWKNSFKYQLSEDWRLIGKFNYAVSDSSLGDSYSGDYTEAALGSAY
                     RPVRNDRLNALFKYTYFYNVPAADQVTGANTASDHIQRSHIGSVDVMYDLTSRWTVGG
                     KYAYRLGQVAQDRIHREFFDSRASLYVLRADWHFVHRWDALVEVRMLDLPDADDSRSG
                     VLVGIYRHVGNHIKAGVGYNFSDFSDDLTQLDYKHQGVFVNLVGKY"
     gene            7103..7618
                     /locus_tag="VLE22_11140"
     CDS             7103..7618
                     /locus_tag="VLE22_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15005.1"
                     /translation="MLRWRRGIISGISVLLLSAAAYGQEISKEQIKGLDEQVQEVKSD
                     VLGIAAELNQLEEKLLYPSNTQVAVFVSLAGGETFRLDSVEIQLDGQPVAHHLYTYKE
                     LEALRKGGVQRIYAGNARSGEHDLQVLVLGKTEGGSDFKKSGRFKVDKNVGPKIVEIS
                     LAAKDITLNDR"
     gene            7622..9508
                     /locus_tag="VLE22_11145"
     CDS             7622..9508
                     /locus_tag="VLE22_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15006.1"
                     /translation="MKRLSVLLTILLFSASPASAGTAAPKQLNDLHFGEALYHAFQGD
                     WFDAISRLDAELVQHYGVDEPERDTLHYHVGQAKFDVGDFELAYRMHRRAGRAFTAVI
                     EGNVEEPVRNEAIFRLARLYFQKDQPVNAQDAVESVRGAVPPEIRNDLDFLRAQIHMA
                     NGRFAEAVRILRDLQNAKGLEGFAAYNLGIALIKDGKELEGRQVLDRTGQINSKEELT
                     LAIKDKANLVLGFKLFKEKNFEGAKLVLDRVRMNGPFSNRALLASGWADASKERFERA
                     LVPWSALVTREVTDAPVQEAMLTVPYAYGKLNVNSKAAVMYGSALEKFDTEVDRLEAS
                     IRSVREGHFLQALAREEMKQDADWVVKLRKLPQTPETYYLLDLMASHDFQESLKNYLD
                     LEGLRRRLEVWERDLDAFEEIIEQRRAYYQPLLPAIDREFRRLDSQMRLRLEQRERIE
                     QRLKAMLVVPRPDYLATAGERVIGERIALLERERTAGGTAGPGGVEARIRRLRGVLHW
                     NIYTEYDRRFTEAYQHLRDLNREVDLLKGQYAAFVRTRQAATQSYEGYDDVIRRQRSL
                     IGNAREKVGELMARQGQMLETMAVNELNARRERVAEFQTKARFTMADSYDRAIRTQTR
                     EMGE"
     gene            9509..>11350
                     /locus_tag="VLE22_11150"
     CDS             9509..>11350
                     /locus_tag="VLE22_11150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB15007.1"
                     /translation="MNRIRLILCAAPWILMLAACATVGDRNGDTIASLRHGHIEIKEE
                     KIEGGLEKAMLSYQRFLEGTPDSALTPEAIRRLADLKIEKEYGTLTTGAGTAGRAKAT
                     PVPEHVTRPKAVSAAGAPSGQALAHIPVHEESETDFERRTTGRQPADDTAAAADRSVE
                     GAGDLERAGAREALALYRKLLDDYPRYERNDQVLYQMSRAYEELGQIEEAMAVMDRIV
                     REYPRSRYIDEVQFRRAERFFAHRRYRDAENAYAGIVAVGEGSSFFELALYKLGWTYY
                     KQEFYEEALDRFIALMDHKVSAGYDFEQTGDEQERKRTDDTFRVISLSFSNLGGADSA
                     VEYFSRHGKRGYEDKVYGNLGEFYFDKRRYADAAAVYKAFMERNPFHKTAPNFHMRVI
                     EIQAAGGFPSLVLDSKKAFATTYGLQAEYWKHFAHADRPDVLGYLKTNLTDLANHYHA
                     CYQDPKQAEDKSANFEEARRWYRGFLTSFPAVPESPVINYQLADLLGENHSFGEAAVE
                     FEKTAYDYPPHEKSSQAGYAAVASYREQLGAARPKDAETVKREVVRSSLKFADAFPKH
                     EKAAIVLGAAADDLYGMKEYEPARAAATKLIEAFPGTDGDVVRDAWLV"
BASE COUNT         2307 a         3518 c         3620 g         1905 t
ORIGIN      
        1 ctcgccatcg gaacgacgat caccaatacc ggcgtggtcc gctggaacga tccggcgcag
       61 accgcgtcgg ccagcgtctc catcgacgtg ggcgggactc cggacagcgc cagtctcaac
      121 ggaaatgtct ggcacgacgc aaatctgaac aagctccgcg agtgcaacag cgaaaactgc
      181 gcgttgacgg gctggactgt cgaactctat cgcaacagtc agctggtcgc caccgttctg
      241 accgacgcca gcggcgccta tcggctcagc ggcttggcgc cgaacgcggg cacgtccgac
      301 ctctatgagc tccgcttccg cgcgccgggc gcgggcccga acacggcctc tatgggtcag
      361 gccgactcgc cgttcaccaa cggcccgcag cggatcagcg gcatcaccgt cgccccgggg
      421 gccaatctgc agaacctcaa tctgcctctc tggcccaacg gcaccgtgta caactctgtc
      481 gcgcgcgtac cggttgccgg agccagaatg acgatgcgga atgcggcgac cggagccgta
      541 ctgccggtcc aatgcttcga ggacccggcc cagcagagcc agattacggc cgcgaacggc
      601 ttttacaagt tcgacctgaa cttcagcgac gcgtcctgcc cggcaggggg cggctatctc
      661 atcgaggtga cgccgcccgc caccggctac ttgaacgggc cgtcccggat cgttgcgccg
      721 tccagtgacg cgcagacggc ggcgttctcc gttcccgcct gccccggcag cgcagccgat
      781 gcggtgcccg cgacccccga gtactgcgag gttgtggcct ccccggcggt gccgccgctt
      841 tcggttccgc cgggcacggc cggcaccgtc tatcatctgc acctcttgct gagcaatgga
      901 accgtgcccg ggcagagcca ggttttcaac aaccagatcg ccatcgatcc ggaactcttt
      961 ggggccgtgg ccatcagcaa gacctcctct ttgaccaacg tgagcaaggg gacgctggtg
     1021 ccctatacga tcaccgtgac caacgtgtac ggtgcgccgc tgtccggcat cggcatcgtt
     1081 gaccgattcc cggccgggtt caaatacgtg gccggctccg cccgcctgga cggcaatccg
     1141 gccgaaccgc tgatcaacgg tcgggagctt gcctggaacg gcctcgaact gcagtttaac
     1201 cagagacgca ccatccggtt tctgctggtg gtgggctccg gcgtctccga agacgagtac
     1261 gtaaaccggg ccttggtgcg caacgccaca accggcgccg caatttcggg ggaggcgacg
     1321 gccacggtga gggtcatccc cgacccggac ttcgattgca ccgatgtcat cggcaaggtg
     1381 ttcgacgatg ccaacctgaa cggcgagcag gattccggcg agaagggact gttcggggtc
     1441 cgcgtggtga cggcccgcgg tctgatcgcc tccaccgata ataacggacg ctttcacatc
     1501 acctgcgcgg cagtcccgga cgaggatcgc ggcagcaact tcatcctcaa gctggacgag
     1561 cgcagtctgc ccagcggcta tcgcctgact accgaaaatc cgcgcgtgca gcgggccacc
     1621 cgcggcaaga tgctgcgctt ccgcttcggc gcagccgttc accgagtggt tcgcctggat
     1681 atcgccgacg gggcgttcga gcccgatacc accaaaatca ggatgcagtg gactcacaga
     1741 atcgaccagt tgcttgaaga gctgaggaag aagccgtccg tgctccggct gtcctatctt
     1801 ggcgacgtcg agcccaaagg caaggccctg gaacgtcttg aagccctgaa caaggaaatc
     1861 accgcacggt ggaagcagtc gggcgccgga tacccgttga ccatcgagac cgaaatcttc
     1921 tggcgacgcg gcgctccgat tgccggtcac cgatagagga ttgatgaaat ccatgggacg
     1981 tcacctgcgc atcgtgaaag gcttcctttt gaggcacggc atcgccgtcc tgtgcgtgtg
     2041 cggcgtcact gcggcggccc ccggggttgc atcctccgag gaaacggggg taagggaaac
     2101 ccatgtgcgc ggagaagcgg ccgaaaggca cctgccggcc gaccggcctt tgatcccatg
     2161 ggtccatgac cccgccgtat ttgaaaagga cgagggggac aggaaggaac tgcgcgaagt
     2221 cgacgagaag gaagtcacca ccatcaagct cgacaacctg gtcccgccca tccatttcgg
     2281 cctggggcaa atcgaaatca ccgaggacta tctgaagatg cttcgcgatg tgctcgacgg
     2341 catgcgcggt cgcgccaatg tgcgactcca ctttattggt catgccgata cccttccgct
     2401 caggggcgag ttgatccata tctacggcga caacatcggg ctgtcacgcg aacgggcggg
     2461 gaccgtggcc gaatactgcc agcgcgcgct gaaccttcct cccgaggcca tctcctacga
     2521 ggggcttggc gacagtcggc cggttgccag caatgcgacg gaagaagggc ggcagctcaa
     2581 ccggcgcgtg gaagtgcagg tctggtacga cgagatcggt cataagcagg tcatgaagga
     2641 agtggtcgtt ccccgcgaaa ggaaccggat caaggtctgc cggaccgaga ccgtctgcaa
     2701 attgcgctac aaggacggcc acgcccgtcg cgcgcgcatc aagaacctga cttcccccct
     2761 tcattacgac aaaggcatgc tgagcgtgcc ggaggaattt ctgcagcagg tcagacgggc
     2821 gcgcaagaat ctcggcgaca aacggaatct tgtggtcaag ctcaccggct atacggacaa
     2881 catcccgttg aagggacggg acgaacgcat ctacggcgac cccgtcggcc tctccaaagc
     2941 ggttgcccgg cgcgttgccc tcgccgtcca ggaggatctg gacttgccca gtgacgccat
     3001 cgataccgag ggccgtgggg cgtcgcagcc cgtggcccca aacgatacgg aacaggggcg
     3061 ggcgctgaat cggcgcgtgg aagtggagtt ctggcacgac gatccgctgc aggacctgcc
     3121 cgaggaaccc cagctgtgcc cggacgcccc cggcgccgag accgtcaccc gtgtgtatga
     3181 ttcgccgtcg ggcggcatcg acccgatcct gtttgaaaac ggcaaacccg tgataccgga
     3241 cggttacacc gggcatttgc ggcgcatcat ggacgagata cacgacagga cccatgtccg
     3301 cctgcggttt gtcggctata ccgccaacaa gctcctcgac cggcgtaccg ccgcggtcta
     3361 cggagatgac atcgggttat ccatggcgcg cgcacgccgg tccatggcgg ccgtccgcac
     3421 gcagatggga ctgtcggaaa gacaggcgga gttcgacggc cgcggttatg tgcagtccga
     3481 cgacgtggtc aacgccggtt ttatcgagtc cgagacgtcg cgcgtcaagg tgcaggtggt
     3541 ctacgatgaa ctgattcccc gcgacgacta cgagggggtg gaggtcaccc cgttgattcg
     3601 cgaagtgaag acggtagatc ccttcgccct gaatctcatg cgcatcacgg tggacggcaa
     3661 gccggtcgat gatccgagca agtgcagttc ggacgtgcag cgctgcatcg atgtggccct
     3721 ggacaacgcc caaatccagt tcaagcacga cagccttacg cttacgccgc ggctgaacgt
     3781 gaccgcatgg ccgactacca tccgttatca ggacgcgccg gatacggcgt ttgccgaaaa
     3841 cctggtgcat ttccgcctgt acctcaacta ccgcagcttc atcgaacggg cggaggtcag
     3901 gattttcgag gagcagcagt ccgtccgcga cacccccctc gccgtcgtcg agatggatgc
     3961 cgacggcagg gcccagtggc aggccgagtt cgagtccttc tccgcgccgg tgcgcaaatt
     4021 gaaatacctg gtgcgggtgg tcgaccggaa gggactcttt gacgagacga cgccgcagcc
     4081 cctgtgggtg gcggaccgca tcgacccgtc ggttgccgag gcgaaggtcg acgaaaaatt
     4141 gcttgcgggg tatggcgaga gccgcatcgc cagctggaat atccgccttc acggcggtac
     4201 ggtgcaggca tacggcaccg cgatccccaa aggacgggga gtgtggatgg ccggctattc
     4261 ggtgcccgtg gacgagaagg gcggtttcgt ggcggaagag atcctgccgg agggcatgca
     4321 taccgtggag gtggcggtcc ttgacaagtc cggaaacggc gagatctttc tgcgggacct
     4381 ggagctgaaa aagagcgact ggttcaccgt ggggatcgcc gacctgaccc tgtccggcaa
     4441 caagacggac gggccggccg aactcctcgc gccggacaaa ccccggtaca gggatgacat
     4501 gagcctcgag ggccgcctgg ccttctatac gcacggcaag ctcgaaaacg gctggtggtt
     4561 cacggcgagc gccgatacgc gcgaaggtcc cctggacgag atcttctcca acttcctgga
     4621 caagtccccc gaggcgctgt tccgacgaat cgacccggac taccactacc cgaccttcgg
     4681 cgatgacggc accgtcctgg aggatgcgcc gaccaccggc aagttcttcg tcaaggcgaa
     4741 gaaggataat acgtacgggc tctggggcaa cttcaaggtc gcgtacactg atacccaatt
     4801 ggcgcacgtg gaccgggggc tctacggcgc aaatctccat tatcaacccc tcggcaccac
     4861 cagtttcggc gagccgcgcc tgctggtgga cgggtttgcc gcggacccgg gaaccgtggc
     4921 gggacgcgat gagttccgtg gcacggacgg atcgctctat ttcctgcgcc ggcaggacgt
     4981 gctggaggga tccgaacgcg tgcgcatcga ggtccgcgac aaggactccg ggatcgttct
     5041 tgcggtcaag aacctgacgc cggttctcga ttacgacatc gactacctgc agggacgcat
     5101 cctgctttcg cagcccttgc ccgcaacggc ggacgacggc ctgttggtgc acagcgcctc
     5161 catcagcggg catccggtct tcctggtggt ccgctacgaa ttcaccccgg gggtcgacga
     5221 accggacacg ctggctgtcg gcggcagggc gcattactgg ttcaatgacc acgtcaaggt
     5281 cggcgtgacc gccagtcatg atgaagaagc cgacatcgaa aacagcctgg gcggagcgga
     5341 cctgaccctg cgcaagtcct cggcgtcgtg gctgaaactg gaagcgggcc gcaccaaagg
     5401 tccgggcgtt ccggcgacga cttccatcga cggcggtttc ggtttcgaca atacggcggg
     5461 tttcccggtc ggcaacgaag tggatgccat ggcctatcgc gtggatgcca gtttgggctt
     5521 caaggacttc tttaaaaagg gacgcggcaa ggtcaccttc tactggcagg acctcgaggc
     5581 gggctattcg gccccgggcc aggctaccgc gcgggacctg acccaatacg gcggaacggc
     5641 ggagataccg tttaccgacc gtctgagcgc gcgcgcgaag atggacaaga gggaggagca
     5701 ggaaggtctg cagaccgaaa ccgcagagct gaacgtcgac taccggatgg ggaagcactg
     5761 gaccctgggt tcgggggtgc gtcacgaccg ccgcaaagac gattcgccgg cggttcccgc
     5821 gacccaggaa gagggcgaca ggacggatgc ggcggtccgg ctgctctacg attcccttaa
     5881 gcgatggaag acctacggtt tcgttcaggg gacggtccag tcaagcggca cccaggaaga
     5941 gaacgaccgc atcggcgccg gcggcagtgt gcgtgtgacc gaccgggtca acgtcaccgg
     6001 cgaggtttcc ggaggagacc tcggtacggg cggcaagctc gtgaccgagt atctgtatac
     6061 cgaccggacc accctgtacg gcaattacag cctcgaaaac gaacggacgg acaacggact
     6121 gcttgccagg aagggcaaaa tggcttccgg tttccgcacc cggtattcgg acagcgccag
     6181 tgtctacctc gaagagcggt atacccacgg cgacgtcccc acgggcctga cgcatgccgc
     6241 cggcgtaaaa ctcgtgcctt tcgaccggct caatttcggc gccaacatcg attttgggac
     6301 cctcaaggac ccccgaacgg cggccgaact caagaggacg gcgctgggcg tgagcgcagg
     6361 ttacgggttc gccaggctgg cgctcgccac cgccgtggaa tatcgggtcg acgacatcga
     6421 gcagccggat accagcttct ccaaacggac ctcctggctg tggaaaaaca gtttcaagta
     6481 ccaactgtcc gaggactggc ggctcatcgg caaattcaat tacgccgtga gtgacagttc
     6541 gttgggagat tcttatagcg gcgactatac ggaagcggcc ctcggctctg cgtatcgccc
     6601 cgtccgcaat gaccgcctga acgcgctgtt caagtacacc tatttctaca acgttccggc
     6661 ggccgatcag gtgaccggcg ccaatacggc gtccgaccac atccagcgca gccacatcgg
     6721 atccgtggat gtcatgtatg acctgacgtc ccgttggacc gtgggaggca agtacgccta
     6781 ccgccttggc caggtggctc aggatcgcat tcatcgggag ttcttcgaca gccgggccag
     6841 cctgtacgtg ctgcgcgcag actggcactt cgtccaccga tgggacgcat tggtcgaggt
     6901 gcgcatgctg gacctgcccg acgcggacga cagccgcagc ggggtgttgg tggggatata
     6961 ccgtcatgtg ggcaatcata tcaaggcggg agtcggctat aacttcagcg atttctcgga
     7021 tgatctgacc cagctggatt acaagcacca gggcgttttc gtcaatctgg tcgggaagta
     7081 ttagagcttg gaggaggaca acatgcttcg ctggcggaga gggatcattt cagggatttc
     7141 cgtgcttcta ttgagcgccg ccgcttacgg gcaggaaatc tcaaaggaac agatcaaggg
     7201 cctggacgag caggtccagg aggtcaagag cgatgtcctg ggcatcgcgg cggagctgaa
     7261 ccagctggaa gagaagctgc tgtatccgtc caacacgcag gtcgcggtat tcgtgtcgct
     7321 ggccggcggc gaaaccttcc gtctcgactc ggtggagatc caactggacg gccagccggt
     7381 cgcccatcac ctttacacct acaaggagct cgaagccctg cggaagggag gggtgcagcg
     7441 catctatgcc gggaatgccc ggtccggcga gcacgacctc caggtcctgg tcctcggcaa
     7501 aaccgaagga gggagcgatt tcaagaaatc ggggcgattc aaggtcgaca agaacgtggg
     7561 cccaaagatc gtggagatct ctctggccgc caaagacatc actctgaatg acaggtagac
     7621 catgaagagg ctgtccgttt tattgacgat tctgttattt tccgcgtctc cggcgtcggc
     7681 cggcacggcc gccccgaagc agctgaacga cctccatttc ggagaggccc tctatcacgc
     7741 gtttcagggg gactggttcg acgccatctc caggctcgac gccgagctcg tgcagcacta
     7801 cggggtggac gaacccgagc gcgataccct tcattaccat gtcggccagg cgaagttcga
     7861 cgtgggcgat ttcgagctgg catatcgaat gcatcggcgc gccggtcgcg cctttacggc
     7921 ggtcatcgag ggcaacgtcg aggagccggt gcgcaacgag gccatcttcc gcctggcgag
     7981 gctctacttt cagaaggatc agccggtcaa cgcgcaggac gccgtggaga gcgtccgcgg
     8041 ggccgtcccg ccggagatcc gcaacgacct ggatttcctg cgcgcccaga tccacatggc
     8101 caacggacgt ttcgcggaag ccgttcgcat tctgagggac cttcagaacg ccaagggcct
     8161 cgagggcttt gccgcttaca acctcggcat cgcactcatc aaggacggaa aggagctcga
     8221 aggccgccag gttcttgacc gtaccggcca gatcaacagc aaggaggagc ttacgctggc
     8281 gatcaaggac aaggcgaacc ttgtcctcgg gttcaagctt ttcaaggaga agaatttcga
     8341 gggagccaag ctggtgctcg accgggtgcg catgaacggc ccgttttcca accgggcgtt
     8401 gcttgcctcg ggatgggcgg acgcctccaa ggaacgattc gagagggcgc tggtgccatg
     8461 gagcgcgctg gtgacccggg aggttacgga cgcgccggtg caggaagcca tgctcaccgt
     8521 cccctatgcc tacggcaagc tgaacgtgaa cagcaaggcc gcggtgatgt acggcagcgc
     8581 actggagaag ttcgacaccg aagtcgacag gctcgaggca tccatcagga gcgtccgcga
     8641 aggccatttt ctccaggccc tggcacggga ggaaatgaaa caggacgccg actgggtcgt
     8701 caagctgcgc aagctccccc agactcccga gacctactac ctgctcgacc tgatggcctc
     8761 ccacgatttc caggaatccc tgaaaaacta cctcgacctg gagggattgc gcaggaggct
     8821 ggaggtgtgg gaacgggatc tggacgcctt cgaggagatc atcgagcagc gcagggcgta
     8881 ttaccagccg ctgcttcccg ccatcgaccg ggaattccgg cggctcgact cccagatgcg
     8941 cctgcgcctg gagcagcggg agcgcatcga acagcggctg aaggccatgc tggtggtccc
     9001 gaggcccgat tatctggcga ccgccgggga aagagtcatc ggcgaacgga tcgcgctcct
     9061 ggagcgggag cggaccgcgg gcggcacggc aggtcccggc ggggtcgagg cccgcatccg
     9121 acgtttgcgg ggagtgttgc actggaacat ctatacggaa tacgaccgtc gcttcaccga
     9181 ggcctatcag catctccgcg acctgaatcg agaggtggac ctgttgaaag ggcagtacgc
     9241 cgcctttgtc cgcacccgcc aggccgccac ccagagctac gaaggttacg acgacgtgat
     9301 ccgccgccag aggtcgctga taggtaacgc ccgggaaaaa gtgggcgaac tgatggcgcg
     9361 tcagggccag atgctcgaaa ccatggccgt caacgaactc aacgcgcgcc gtgagcgggt
     9421 tgccgagttc caaaccaagg cgcgtttcac catggcggac agctatgacc gtgccatcag
     9481 gacccagacc cgggaaatgg gtgaataaat gaaccggatc cgactcatcc tatgcgccgc
     9541 gccatggata ttgatgctgg ccgcgtgtgc cacggtcggc gacaggaacg gggataccat
     9601 cgcctcgttg cgtcatgggc atatcgagat caaggaggag aagatcgagg gcggactcga
     9661 gaaagcgatg ctgagttatc agcgctttct ggagggcacg ccggactccg ccttgacgcc
     9721 cgaggccatt cgccgcctgg ccgacctgaa gatcgaaaaa gaatacggca ccctcacgac
     9781 gggcgccggg accgccgggc gggcgaaggc aacgcccgtc ccggaacacg taacgcgtcc
     9841 caaggccgtg tcggccgccg gcgcaccgtc gggccaggca ttggcgcata ttccggttca
     9901 cgaggagtcc gagacggact ttgaaagacg gacgaccggg aggcagccgg cggacgacac
     9961 ggcggcagcc gccgaccggt ctgtcgaagg agccggcgat ctcgaaaggg cgggcgcccg
    10021 cgaggccctc gcgctttacc ggaagctcct cgacgattat ccccggtacg agcgcaacga
    10081 ccaggtgctg taccagatgt cccgcgcgta cgaggagctg ggacagatcg aggaagccat
    10141 ggcggtcatg gaccggatcg tccgcgagta tccccggtcc cggtatatcg atgaagtgca
    10201 gttccgccgc gccgaacgct tcttcgcgca caggcgctac cgggatgccg agaatgccta
    10261 cgcgggcatc gtggccgtcg gcgaaggctc ctcgttcttc gagctcgccc tctataaact
    10321 gggctggacg tactacaagc aggagttcta tgaagaggct ctggaccgat tcattgcgtt
    10381 gatggaccac aaggtgtccg cgggatacga tttcgaacag accggagacg agcaggaacg
    10441 caagcggacg gacgacacct tccgggtcat cagtctgagt ttttccaacc tcggcggcgc
    10501 cgattccgcg gtggaatact tctcgcgcca cggcaagcgc ggttacgagg acaaggtata
    10561 cggcaacctc ggcgaattct acttcgacaa gcgccggtac gccgacgccg cagcggtcta
    10621 caaagccttc atggaacgca acccgttcca caagacggcg ccgaacttcc acatgcgcgt
    10681 tatcgaaatc caggccgccg gcggatttcc cagcctcgtt ctcgactcca aaaaggcatt
    10741 cgccacgacc tacgggctgc aggccgaata ctggaagcat ttcgctcacg ccgaccgtcc
    10801 ggacgttctc ggttatctga aaaccaacct gaccgacctg gcgaaccatt atcatgcctg
    10861 ctaccaggac ccgaaacagg ccgaagacaa atcggcgaat ttcgaagagg cgcggcgctg
    10921 gtatcgaggg ttcttgacct cgttccccgc ggtccccgaa tcgcccgtca tcaactacca
    10981 gttggcggac ctgctcgggg agaatcactc gttcggcgaa gccgccgtgg aattcgagaa
    11041 gaccgcgtac gactatccgc cccacgaaaa atcctcccag gccggctacg cggcggtcgc
    11101 ctcctaccgg gagcaacttg gcgccgcccg cccgaaggac gcggagacgg tcaaacggga
    11161 ggtcgtccgc agttcgctga agtttgccga tgccttcccg aaacacgaaa aagcggcgat
    11221 cgtcctcggg gcggccgcgg atgatctcta cggcatgaag gaatacgaac cggcgcgtgc
    11281 cgccgcgacc aagctcatcg aggcattccc cggcaccgat ggggatgtcg tccgtgacgc
    11341 ctggctggtc
//
LOCUS       DATIFQ010000242         4995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_461721, whole genome shotgun sequence.
ACCESSION   DATIFQ010000242 DATIFQ010000000
VERSION     DATIFQ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461721"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..634
                     /locus_tag="VLE22_11155"
     CDS             248..634
                     /locus_tag="VLE22_11155"
                     /inference="COORDINATES: protein motif:HMM:NF018050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSB15008.1"
                     /translation="MRIFKTKAFTRFANKAHVGDGSLCGAVRDAERGLIAADLGGGVI
                     KQRIARPGQGKSGGFRTLIVFRAGVRAIFVHGYAKNEKDTIAKDELSALKKLAAELLA
                     YDDKTIARVIASGTFVEVTCEKKTIP"
     gene            642..932
                     /locus_tag="VLE22_11160"
     CDS             642..932
                     /locus_tag="VLE22_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HSB15009.1"
                     /translation="MASIHETAEGLHSIGVMDKRTMREFDVLCLTQVRPLKPKEIRAL
                     RLREGASQAVFARYLNVTTGLVSQWERGEKHPQGASLKLLTLVAKNGLQAVA"
     gene            complement(1000..2724)
                     /locus_tag="VLE22_11165"
     CDS             complement(1000..2724)
                     /locus_tag="VLE22_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15010.1"
                     /translation="MRMQHLVLIVLGALVCAPGSVLSAELPGDYFKLMESELKSVEPA
                     PELRNPCSYMFAAAVLYTKQHPANPDFGNEKFLELALKLGDVTAEQSERDDFLDRQDY
                     EWEIHFWLDSYRLLEAKLSGERRARWRKQLERNVRWFAGQTEARLDFPRYQGPFIRTS
                     TNHYALFASTVYLAGRVLKEKEWEELGMRAMHRLATEEQAAGGYWGEFTDNGPATGYD
                     YITMNCVALYYEHSLDTAALKALRRSTDFHKYFTWPNGEPVETINGRNRYWSVSPWGT
                     FAFSHWPDGRGYAEFLSQFFSAGKVPARDLGRLSQNALYYHEGPTEPIPQQLSQAAHQ
                     LPVPAGIRRTEPWTVCLSGLYDPPTTSQFTLDRQGNLSIFHDRLGMIITGAGSKYQPE
                     LATIMERAEGEVTTVPRSSRLRMTDALDRIGMAYRRFFVDLQVPRPEPERLTFQFRIT
                     EVGPNQMGEASINLQLVLKPGEVLETAHRKVTLDQNPIDLGPDQVGGWIRHRGWTLKI
                     PPTARLIWPVLPFNPYRNARETDLRYAVGRLLVPLQVEKRPDWALNWGRQDIEFTLEA
                     PSAKPASR"
     gene            2919..3839
                     /locus_tag="VLE22_11170"
     CDS             2919..3839
                     /locus_tag="VLE22_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HSB15011.1"
                     /translation="MALAIALEEEDGKIYGDFEDGLRERYPASARLFEEMQAEESSHR
                     ASLLDLYRAKFGDHIPHIRRQDVKGFVERKPVWLVRPLGLQAVRKQAEIMELETRRFY
                     EHAAQQVTDVSVRKLLGDLAQAERRHSALAESLEEKLLTPDVIKQEETAQRRLFVLQI
                     VQPGLAGLMDGSVSTLAPVFAAAFATKNSWDAFLVGMAASVGAGISMGFAEALSDDGS
                     LTGRGHPWIRGLVCGLMTTAGGIGHTLPFLIADFHAATVVAIVVVTAELFAISLIRNK
                     YMDTPFVSAALQVILGGALVFAAGILIGSS"
     gene            complement(3875..4822)
                     /locus_tag="VLE22_11175"
     CDS             complement(3875..4822)
                     /locus_tag="VLE22_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSB15012.1"
                     /translation="MAGKPTVAAEECSALGAPDRTLESFPNTNVMLGNLPVARALPVR
                     GRRLVGPWCFLDRFGPLTFADEKPMDVAPHPHIGLQTVTWLLDGEILHDDSLGCEAVA
                     RPGGVNVMTAGAGIAHAEQTPLDNSSHLDGVQLWVALPEAHRNIDPAFEGLTLVPGVE
                     QPGGVIQVFAGSLAGAASPARHYSGIVGADVQVHPGEAVEIGLEQGYEHAVLVLRGDC
                     SLEGRPLPDHQLHYLGVRRSSVSFRSRSGARILLIGGPPFPETVLMWWNFVARTPDEI
                     ARAREDWEQHQRFGEVSAYRGPRLAAPDLVRFARPNLVS"
BASE COUNT          978 a         1518 c         1566 g          933 t
ORIGIN      
        1 tgcccacgac cgtggtgatc gaccgccaag ggaaggtcgt caaacagtgg atcgagtttg
       61 cgagcggccg aaaggtacgc cggatcatcg agactctgat cgcccggtag ccggtaccgt
      121 gtaacctgtt gagtccagca cccggcgtcg cgtcaccaaa gagctgcctc gtggccgtcc
      181 gttatctata gcaccaagtg ttatactgac tccttgagca ctgcaaaggg caatcctggt
      241 aaggatcgtg cgaatattca aaaccaaagc gttcacgcgc ttcgcaaaca aggcgcacgt
      301 tggcgatggc tcgctttgcg gagcggtacg ggacgcggag cgaggattga ttgccgccga
      361 cctcggcgga ggagtgatca agcagcggat cgcgcgcccc ggccagggga aatcgggcgg
      421 ttttcggacg ttgatcgtat tcagggcggg tgtgcgagcc atcttcgttc acggctacgc
      481 gaagaacgaa aaggacacca tcgcaaaaga cgaactgtcc gccctcaaga agctcgcagc
      541 agaattgttg gcctatgatg acaagaccat cgcacgggtg atcgcgtcag gaactttcgt
      601 ggaggtgacc tgtgagaaga aaacaatacc gtagtccatt gatggcgtcg atacacgaga
      661 ccgccgaagg tttgcattcc atcggagtga tggacaagcg cacgatgcgc gaattcgatg
      721 tgctatgcct aacgcaagtg cgcccactga agccgaagga aatccgcgcg ctccgtttgc
      781 gcgaaggggc cagccaggcg gttttcgccc ggtatctcaa cgtgaccacg ggcctcgtca
      841 gccagtggga acgcggtgaa aaacacccgc aaggagcttc gttgaaactg ctcactctgg
      901 tcgccaagaa cgggctccaa gcagtcgcgt gatgtggcgg gacacaacgc caacagcttc
      961 ggtttccccg aacatccgga aggagggagt tgcagagcgt cagcgtgacg ccggtttggc
     1021 cgaaggggcc tccagcgtga actcgatatc ctgccgtccc cagttcaagg cccagtccgg
     1081 ccgtttctcg acctggagcg gcaccagcag ccggccgact gcgtatcgca aatcggtctc
     1141 acgggcgttg cggtacgggt tgaagggcag caccggccag atcaggcgcg cggtgggagg
     1201 gatcttcagc gtccagccgc ggtggcggat ccagccaccg acttggtccg gccccagatc
     1261 gataggattc tggtccaacg tgaccttcct gtgagcagtc tccagcacct cgccgggctt
     1321 gaggacgagc tgcaagttga tggaggcttc ccccatctgg tttggcccca cttcggtaat
     1381 tctgaattgg aaggtcaggc gttccggctc cggacgggga acctgcaggt ccacgaagaa
     1441 cctgcggtac gccatgccga tgcggtccag cgcatcggtc atccgcaacc gactgctccg
     1501 ggggacggtg gtcacctcgc cctcggccct ctccatgatc gtcgccaatt ccggctggta
     1561 cttcgagccg gcgccggtga taatcattcc cagccggtcg tggaaaatgc tcaggtttcc
     1621 ctgccggtcg agcgtgaact ggctggtggt gggtgggtcg tacaggccgg agaggcagac
     1681 tgtccacggt tcggtccggc gaatgccggc cggcaccggc agctggtggg ccgcctgaga
     1741 caactgttga gggatcggtt cggtaggtcc ttcgtgataa tagagcgcgt tttgtgaaag
     1801 ccgccccagg tcgcgagcgg gcaccttacc ggcgctaaag aactgcgaga ggaattcggc
     1861 gtacccgcgg ccgtccggcc agtgcgaaaa ggcgaaggtc ccccacggac tgacgctcca
     1921 gtaccggttg cgaccgttga tcgtctccac gggttcgccg ttgggccacg tgaaatactt
     1981 gtgaaaatcg gtcgatcggc ggagggcttt cagcgccgcg gtgtcgaggc tgtgctcgta
     2041 gtacagggcg acgcagttca tggtgatgta atcgtaaccg gtggccggcc cgttgtcggt
     2101 gaactcgccc cagtagccgc cggcggcctg ctcctcggtg gccagccggt gcatggcgcg
     2161 cattccaagt tcctcccatt ccttttcctt gaggacccgt ccggcaaggt agacagtcga
     2221 ggcgaacagt gcgtaatggt tcgtggacgt ccggatgaac ggtccctggt agcggggaaa
     2281 gtcgaggcga gcttccgttt ggccggcgaa ccagcggaca ttccgctcca actgtttcct
     2341 ccagcgagcg cggcgctcgc cggacagttt cgcctcgagc agacggtagg aatcgagcca
     2401 gaagtggatc tcccactcgt agtcctgccg gtccaggaaa tcgtcccgct cgctctgttc
     2461 ggccgtcaca tccccgagct tcaacgccag ctccagaaac ttctcgttcc cgaagtcagg
     2521 gttggccgga tgctgcttcg tatacagcac cgccgcagcg aacatgtaac tgcaggggtt
     2581 gcgcagttcc ggcgcaggtt cgacggactt cagctcactc tccatcaact tgaagtagtc
     2641 gcccggcagc tcggcgctca agacgcttcc cggcgcgcac accaatgcgc cgagcacgat
     2701 aaggacaagg tgttgcattc tcatgatggt tcccccgact cctctggtca tggactgacc
     2761 agttcgcagc ctggcttccc ggcccggctt cctccgattc ccaaccacga taatcataaa
     2821 ccggacggac cacccgtgcc cgcgctgccg tgccggatgc cggaggctca gataatggaa
     2881 ccatggccaa aaacttcaga gatctctagc gggagattct ggctctcgcg atcgccctcg
     2941 aagaggagga tggcaagatt tacggcgact tcgaggatgg cctccgcgag aggtacccgg
     3001 cctccgcaag gctcttcgag gagatgcaag cagaggagtc cagccaccgc gcgagtttgc
     3061 tggacctgta ccgcgcaaag ttcggcgatc atatcccgca catacgccgc caagatgtaa
     3121 aaggattcgt cgagcgaaag cctgtgtggc ttgtgcgccc cttgggactg caggcggttc
     3181 gcaaacaggc cgagatcatg gaactggaga ctcgccggtt ctatgaacac gccgctcaac
     3241 aggtgaccga tgtttcagtt cgaaagctgt tgggagatct cgcgcaggcc gaacgcaggc
     3301 attccgccct cgcggaatca ctcgaagaga agctgctgac cccggatgtg atcaaacagg
     3361 aagagacggc gcagcggcgg ctttttgtgc tgcagatcgt acagcccggc ctggcgggct
     3421 tgatggacgg ctcggtctcg acgctggcgc ctgtgtttgc cgcggccttt gccaccaaga
     3481 acagttggga cgcgtttttg gttggcatgg cggcatccgt tggcgcgggg atttcgatgg
     3541 gctttgcgga ggccctctca gacgatggtt cgctgaccgg ccgcggccat ccgtggatac
     3601 gcgggctggt ctgcggcctt atgacgactg ctggcgggat tggccatacg ctgcccttcc
     3661 tgatcgccga ttttcacgcg gcgacagtgg tggcgatcgt tgtcgttact gccgagttgt
     3721 tcgccatcag cctgatccgc aacaagtaca tggacacgcc tttcgtctcg gcggcgttac
     3781 aggtgattct tgggggcgca cttgtgttcg ccgccggaat tctgatcgga agttcgtaag
     3841 gggcgtcaac aggcgggcat ccggacgccg gatgctaact gacgagatta ggccgcgcga
     3901 aacggaccaa gtctggcgca gccagcctcg gtccacgata ggcgctcacc tctccgaaac
     3961 gctggtgctg ttcccaatct tcgcgggcgc gggcgatttc gtccggggtt cgagccacga
     4021 aattccacca catgagaacc gtctccggaa acggcggccc gccaatcagg aggatgcggg
     4081 cgcccgaacg actgcggaag gacacgctcg aacggcgcac acccaaatag tgaagctggt
     4141 ggtccggcaa aggccgcccc tccagagaac aatctccacg gagcacgagc acggcgtgct
     4201 cgtatccctg ctcaagcccg atttccacgg cttctccggg atggacttga acatccgcgc
     4261 cgacaatccc ggaatagtgc cgcgcgggag atgccgctcc cgcgagcgaa ccggcaaaca
     4321 cctgaatgac tccgccaggc tgctcgacgc ccggcacaag cgtgagtcct tcaaaggccg
     4381 gatcgatgtt acggtgcgcc tccggaagcg cgacccaaag ctgcacaccg tccaagtgag
     4441 aactgttgtc aagcggcgtc tgctccgcgt gagcaatgcc ggcgccagcc gtcatgacgt
     4501 tgacgccgcc cggccgcgcc accgcttcac acccgaggct gtcgtcgtgc aggatctcgc
     4561 cgtcgagcag ccacgtcacc gtttgaaggc cgatgtgagg atggggcgcc acatccattg
     4621 gcttctcgtc cgcgaaggtc agcggtccaa accggtcgag gaagcaccat gggcccacga
     4681 gccgccggcc tctgaccgga agcgcacgcg caaccgggag gttgccaagc atgacattgg
     4741 tgttcgggaa cgactcgagt gtgcgatccg gcgccccgag cgcgctacac tcttccgccg
     4801 cgacagtcgg tttcccagcc atgcatgaac tcctctcgtt tccaggctcc gcgtctgacg
     4861 acctaggccc agaatagcaa cggatgacgg ttaccggagc tgttgagtga cccgaagctt
     4921 actcggatct gaggccggtc cggctacaat gtagagcaca ggtttatgcg ggtctggttc
     4981 ggcacccaaa tcctc
//
LOCUS       DATIFQ010000243         7480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1802341, whole genome shotgun sequence.
ACCESSION   DATIFQ010000243 DATIFQ010000000
VERSION     DATIFQ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802341"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /gene="scpB"
                     /locus_tag="VLE22_11180"
     CDS             <1..424
                     /gene="scpB"
                     /locus_tag="VLE22_11180"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HSB15013.1"
                     /translation="HHEAIRGFVKNLKPPLKLSLPALETLALIAYKQPITAPEIMEIR
                     GVQGAGVLKTLLDRKLITTAGRKNVVGRPILYKTTRDFLVQFGLKDLSELPTLKEFEE
                     LGRLAVADEEPATAETAEQSEPAEPSEEVPPAEEEQAE"
     gene            425..1156
                     /locus_tag="VLE22_11185"
     CDS             425..1156
                     /locus_tag="VLE22_11185"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSB15014.1"
                     /translation="MAEERLQKILSRAGVTSRRKAEELMLEGRVTVNGAVVVTLGTKA
                     DLARDEIRVDRRRLKEPQRLVYLAVNKPDSCVTTLHDPQGRRTVLDLVKGIRERVFPV
                     GRLDYHSEGLLLLTNDGEFANRITSASSHVQKTYLVKVNGNLSLEQEDSFRGGIPLGG
                     RRTAPAGLRLIRRARNPWYEVRLIEGRQNQIRLMFKHLGRLVEKLRRVRIGFLELGPL
                     KPGEFRHLTPSEVDRFRRVLNLKRD"
     gene            1158..1553
                     /locus_tag="VLE22_11190"
     CDS             1158..1553
                     /locus_tag="VLE22_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HSB15015.1"
                     /translation="MPSVLELLRTSRIIAVVGLSSKRWRPSFGVSEYMQRAGYRIIPV
                     NPGETEVLGEKSYGGLEEVPEKVDIVDVFRRSEFVPEIVEQAIRIGARAVWLQEGVVH
                     EEAAAKARAAGLMVVMDRCILKEHRKLVD"
     gene            complement(1563..1916)
                     /locus_tag="VLE22_11195"
     CDS             complement(1563..1916)
                     /locus_tag="VLE22_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSB15016.1"
                     /translation="MATLTIRNRTKGTTLATHADSADTSRKRRVGLLKHESLPPGEGL
                     WIAPCEAIHTFGMKFPIDVLFLNREKKVLKVRQNMPKSRISVSIWAHSVLELPAGAIA
                     ATSTEPGDQLEFSEA"
     gene            2005..3441
                     /gene="rimO"
                     /locus_tag="VLE22_11200"
     CDS             2005..3441
                     /gene="rimO"
                     /locus_tag="VLE22_11200"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HSB15017.1"
                     /translation="MKIGFVSLGCPKNLVDTEVMMGQLVALGHELTPRPEEADAIVVN
                     TCSFIDPAKQESVDTILEMAEFKRSGRARKLIVAGCLVERYRDEIKKNIPDVDAVIGT
                     NELESIAAVCEGTDRGSNPLEAYLYHDLTPRVLSTPRHYAYVKIAEGCDHPCSFCVIP
                     QFRGRFRSRHFESVVAEASRLFSEGVREINLIGQDTTCYGEDLGLQDGLALLLDRLAR
                     IDTPREKWIRFLYAYPNKVTQRLLDTMAGHAALVKYIDMPLQHASASVLKRMKRGASG
                     DIFLKLVERIRRTIPGVAIRTSMIAGFPGETAEDFEELCQFVEAARFDHLGVFSYSDE
                     ETSVSYHLDGKVDRRTIYNRKRRLMALQRRVSRSTNRLRAGEEVTVLVEGPSRETDLL
                     WEARMATQAPEIDGVVLINDFIGTEPKPGQIRRLRITEAHDYDLVGTLLEARESESHP
                     PSPDPFRILGGHTAPPSAALGQGAELQP"
     gene            3438..3629
                     /gene="yacG"
                     /locus_tag="VLE22_11205"
     CDS             3438..3629
                     /gene="yacG"
                     /locus_tag="VLE22_11205"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HSB15018.1"
                     /translation="MKCPICKKEVQPGDPFMPFCSDRCRIIDLGNWATGKYVISTPIV
                     TESEAEEDEAPPKRNGDGR"
     gene            3619..4098
                     /locus_tag="VLE22_11210"
     CDS             3619..4098
                     /locus_tag="VLE22_11210"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HSB15019.1"
                     /translation="MAGSAAGRLASCISTVCGLGRVPVAPGTAGSLGALAVAYALVSV
                     FGWKPWHFAVLSAVIAVPSVWAASRTAMDSGSKDPPKVVIDEVIGQWVTLAGAGLLVW
                     QTWLAAFVLFRLFDIWKPPPVRQAEALPGGAGIIADDVVAGVYGALVLFAAGCFNFE"
     gene            4103..5131
                     /locus_tag="VLE22_11215"
     CDS             4103..5131
                     /locus_tag="VLE22_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSB15020.1"
                     /translation="MAIRKESGNRPKIEAVSPRAAIAGGEFQIRGSGLAKSHQPRVTL
                     GDVPAPIVIGSAEYIVIRVPEGASVGELVIQNGEQASASWMCDLGVQIADSLHPVANP
                     AVDAQGNIFVTFSGSRGQKTPVSVFKIDLNYNVKPFLSELMNATGLAFDSDGQLYVSS
                     RYDGIVYQVTPGGTMSVYVEGMGVATGIAFDGEGNLYVGDRSGTIFKISRDRQIYVFA
                     TIEPSIAAYHLAFGADGHLYVTGPTTSSFDAVHRISPAGDAEVFYRGLGRPQGLAFDA
                     DGNLYVAASMGGRKGVIRIDPERRAELFLSGPGIVGMAFAPSGSLVVATSGALYRADV
                     GIRGKPLP"
     gene            5132..6403
                     /locus_tag="VLE22_11220"
     CDS             5132..6403
                     /locus_tag="VLE22_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HSB15021.1"
                     /translation="MNAEIIAVGSELLTPQRLDTNSLYLTEKLNGIGVEVVAKAVIGD
                     DARRLAEAVRGALARSEIVILTGGLGPTEDDVTRDAVAGALGRELIFHDEIRERIEQR
                     FRRMGRRMVEINKRQAYVIEGAEWLPNDNGTAPAQWIEDSGRVVILLPGPPSELKPLF
                     DQECLPRLKRRLPPLVIRTLFFRVAGMPESDLDQLISPVYKQYENPVTTILAAPGDIQ
                     IHLRARCETEEEAQRLLAEAGGRIAELLGDRIYSRNGDPLEAVVGQRLRERGETLSVA
                     ESCTGGLLAERITQIPGSSDYFLGGFLVYTERMKTELLGVDAALLGEHGAVSEAAAEA
                     MAIGARNRTGSTYALSVTGYAGPDGGDEGAPVGTVFIGLASASGCQARRFRFLGGRDR
                     VRAMAAQTGLDLLRLNSRQRSAISDQQKADS"
     gene            6887..7450
                     /locus_tag="VLE22_11225"
     CDS             6887..7450
                     /locus_tag="VLE22_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15022.1"
                     /translation="MTSNQKIQPVRDRELIDRMRQLAAGVVVGTGDVSREWVLKKHWV
                     VVPVESDDHFSGATVARIAASCRDGKDQCFALITEEVYNIDSCFLVPCEEAALEELNN
                     ALALFPFVLLPQERSWCIVCTKEDYYLVAGPSGFVETVVGMSIADAQAAYRRSADEWI
                     MEKQKQFHRSVAARYEELTSATASNRA"
BASE COUNT         1390 a         2233 c         2504 g         1353 t
ORIGIN      
        1 gcatcacgag gcgatccgcg gcttcgtcaa gaatctcaag ccgccgctca agctctcgct
       61 gcccgcgctc gagacgctcg ccctgatcgc ttacaagcag cccatcacgg cgccggagat
      121 catggagatc cgcggcgtgc agggcgcggg cgtactcaag actctgctcg accggaagct
      181 catcactacc gccggccgca agaacgttgt cggtcggccg atcctctata agaccaccag
      241 ggacttcctc gtgcagttcg gcctcaagga tctgagcgag ttgcccactc tgaaggagtt
      301 cgaggaactc gggcgattgg ccgtggctga cgaagagcct gcgacagccg aaaccgccga
      361 gcagtccgaa cccgccgagc cgtccgagga agtgccgccg gccgaagaag agcaagccga
      421 gtaaatggcc gaagagcgac tccagaagat actctcccga gccggtgtca ccagccgccg
      481 aaaggccgag gagttaatgc tggagggccg ggtcaccgtt aacggcgcgg tcgtcgtcac
      541 cctcggaacc aaggccgatc tggcgcggga cgagatccgc gtggaccgcc gccgtctcaa
      601 ggaaccccaa cggctggtct acctcgctgt gaacaaaccg gatagctgcg tcaccaccct
      661 ccacgacccg cagggcaggc gcaccgtgct cgatctcgtg aaggggattc gcgagcgggt
      721 gtttccggtt ggacgcctgg attatcacag cgagggcctg ctgctgctga ccaacgatgg
      781 cgagttcgcc aaccggatca ccagcgcttc cagccacgtc caaaagacct acctcgtgaa
      841 ggtcaacggg aacctttcgc tggagcagga agactccttc cggggaggca tccctctggg
      901 agggcgacga acggcgccgg ccggcctgcg gttaatccgg agggcgcgga acccgtggta
      961 cgaggttcgg ctcatcgaag gccgccagaa tcagattcgg ctgatgttca agcacctcgg
     1021 ccggctggtc gagaagttgc gccgcgtccg gatcggcttc ctcgaactcg ggccgctcaa
     1081 acccggcgaa tttcgccacc tgaccccgtc tgaggtggat cgttttcgcc gcgtgttgaa
     1141 tctaaaacgt gactgaaatg ccaagtgtgc ttgagctcct gcggacgtcg cggatcatcg
     1201 ccgtcgtcgg tctttcaagt aagaggtggc gtcccagttt tggggtctcc gagtacatgc
     1261 agcgagcggg ataccggatc atccccgtga accccggcga gacggaagtg ctcggcgaga
     1321 agtcctatgg cggcctcgaa gaggtcccgg agaaagtgga tatcgtcgac gttttccgcc
     1381 gctcggagtt cgtcccggaa atcgtagaac aggcgattcg gatcggggcg cgggcggttt
     1441 ggcttcagga gggggtcgtt cacgaagagg ccgcggcgaa agcccgggcc gccggcctga
     1501 tggtcgtgat ggatcgctgt atcctcaagg aacacaggaa gttagtcgat tagctccgcg
     1561 gttcaagcct cgctgaattc gagctgatct cccggttcgg tcgaggttgc ggcgatggcg
     1621 ccggccggca gttcgagcac ggagtgggcc caaatggaca cagaaatgcg gcttttgggc
     1681 atgttttggc gcacttttag cacctttttt tcacggttca ggaacagaac gtcgatcgga
     1741 aacttcattc cgaaggtgtg aatcgcttcg cacggcgcaa tccacaaccc ttctcccggc
     1801 ggcaggcttt cgtgcttgag gagtccgacg cggcgcttcc ggctggtgtc ggcggagtcg
     1861 gcgtgggtcg cgagcgtggt tcccttggtc cgatttcgaa tggtgagcgt cgccattcct
     1921 cacaagatag cggtccgggg ctgatgtgcg ccacttgatg ctattctgga agggaacccc
     1981 atcccttttt gccggaactt catcttgaaa atcggatttg tcagtctagg atgtccgaag
     2041 aatctcgtgg acaccgaggt catgatgggc cagctcgtgg ctctggggca cgagctcact
     2101 cctcgccccg aggaggcgga cgcaatcgtc gtcaacacgt gcagcttcat cgatccggca
     2161 aagcaggagt ccgtagacac gatcctcgag atggccgaat tcaagcgctc cggacgcgcg
     2221 cgcaagctga ttgtggccgg ctgccttgtg gagcggtatc gcgacgagat caagaagaac
     2281 attcccgacg tggacgcggt gatcggtacg aacgaactgg aatcgatcgc ggcggtttgt
     2341 gaagggacgg accgcggatc gaacccgctc gaagcgtatc tctaccacga cctgacgccg
     2401 cgcgtccttt ccacgccccg ccattacgct tacgtcaaga tcgcggaagg ctgtgaccac
     2461 ccgtgcagtt tctgtgtgat tccacaattc cgggggcggt tccgcagccg ccatttcgaa
     2521 tcggtcgtgg cggaggcgtc ccggttgttc tccgagggtg tgcgcgaaat caatctcatc
     2581 ggccaggaca caacgtgcta cggcgaggac ctcggtcttc aggacggcct ggctctcctg
     2641 ctcgaccgtc tcgcgcggat cgacacgccc cgcgagaaat ggatccggtt cctgtacgcc
     2701 tatccaaaca aggtgaccca acggctgctc gacacgatgg ccgggcacgc cgcgctcgtc
     2761 aagtacatcg acatgccgct tcagcacgcg agcgcgagcg tgctgaaacg gatgaaacgc
     2821 ggggcgtcgg gagacatttt tctgaagctc gtcgagcgga ttcgccggac cattcccggc
     2881 gttgcgatcc gcaccagcat gatcgccggc ttccccggcg agacggcgga ggatttcgag
     2941 gagctttgcc agttcgtcga ggcggcccga ttcgaccatc tcggggtttt ctcgtattcg
     3001 gacgaggaaa cgagcgtgag ctaccatctg gacggcaagg tggaccgcag gacaatctac
     3061 aaccgcaagc ggcgcctcat ggcgcttcag agacgagtgt cgcgaagcac gaaccggctc
     3121 cgcgccggcg aggaagtgac ggttctggtc gagggacctt cccgggagac cgatctgctt
     3181 tgggaggcgc ggatggctac ccaagcgccc gagatcgacg gcgtggtcct catcaacgac
     3241 ttcatcggaa ccgagccgaa accggggcag atacgccgcc tccgcatcac cgaagctcac
     3301 gattacgacc ttgtcgggac gctgctcgaa gcgcgcgagt ccgaaagcca ccccccttct
     3361 cccgacccgt tccggattct gggcggccac acggcgccgc cgtccgccgc cctggggcag
     3421 ggagcggagt tgcagccatg aagtgcccaa tttgcaagaa agaggttcag cccggcgatc
     3481 cgttcatgcc gttttgcagc gaccgctgcc ggatcatcga cctcggcaat tgggcgacgg
     3541 ggaagtacgt gatctcgacc ccgatcgtga cggagtccga ggcggaagaa gacgaagcgc
     3601 ccccgaagag gaacggcgat ggccggtagc gccgccggcc ggctcgcgtc ctgcatttcg
     3661 accgtctgcg gcctcggccg cgttcccgtg gcgccgggaa ccgcgggttc tctgggtgcg
     3721 cttgcggtgg cgtatgcgct ggtcagcgtc ttcggctgga agccctggca ttttgccgtg
     3781 ttgtccgccg taattgccgt gccgtcggtt tgggccgcga gccggacggc gatggacagc
     3841 gggagcaagg atccgccgaa ggtcgtgatt gacgaggtga tcggccagtg ggtgacgctg
     3901 gcgggcgccg ggctattggt gtggcagacg tggctggccg cgtttgtgct cttccggctg
     3961 ttcgacatct ggaagccgcc gccggtgcgc caggcggaag cgcttccggg cggggccggc
     4021 atcatcgccg acgacgtcgt ggcgggcgtc tatggcgcgc ttgtcttatt cgcagcgggg
     4081 tgtttcaatt ttgagtgacg ctatggcgat acggaaggaa tcgggcaacc ggcccaaaat
     4141 cgaagcagtc tcgcctcggg ccgcaatcgc cggaggggag tttcagatcc gcggcagcgg
     4201 gctcgcgaaa tcgcatcagc cgcgggtcac cctcggagac gttccggcgc cgattgtgat
     4261 cgggtcggct gagtatatcg tgatccgcgt gcccgagggc gcgtcggtcg gcgaactggt
     4321 gattcagaac ggcgagcagg caagcgcatc ctggatgtgc gacctcggcg tgcagattgc
     4381 cgacagcctg cacccggttg cgaatccggc cgtcgacgca caaggaaaca tctttgtgac
     4441 gttctccggc tcgcgcggcc agaagacgcc ggtgtccgtc ttcaagatcg acctgaatta
     4501 caacgtaaaa cccttccttt cggaactgat gaacgccacg gggctggcgt tcgacagcga
     4561 cgggcagctc tacgtgtcca gccgctacga cggcatcgtc taccaggtga cgcccggcgg
     4621 cacgatgtcc gtctacgtcg aaggcatggg cgtggcgacc ggcatcgcat tcgatgggga
     4681 ggggaacctc tacgtcggcg accggagcgg cacgattttc aagatcagcc gcgaccgcca
     4741 gatttacgtc tttgccacca tcgagccgtc gatcgccgct taccatcttg ctttcggcgc
     4801 cgacggccac ctttacgtga ccgggccgac cacgtcgagc ttcgatgcgg tccaccgcat
     4861 ctcgcccgcc ggcgacgccg aggttttcta tcgcgggctt ggccgtcccc agggcctcgc
     4921 tttcgacgcg gacggaaatc tgtacgttgc cgcgtcgatg ggcgggcgca agggcgtcat
     4981 tcgaatcgac cccgagcggc gcgcggagtt gttcctttcc ggaccgggca tcgtcggtat
     5041 ggcgttcgcg ccttccggct cgctcgtcgt cgccaccagc ggcgcccttt acagggccga
     5101 tgtcggaatc aggggcaagc ctctcccgta aatgaacgcc gaaatcatcg ctgtcggctc
     5161 ggagttgctg acgccgcagc ggctcgatac gaactctctt tacctcacgg agaagctgaa
     5221 cgggatcggt gtggaggttg tcgcgaaggc cgtgatcggc gacgacgccc gccgcctggc
     5281 ggaagcggtg cggggcgccc tcgcacgttc ggagattgtg atcctcaccg gcggcctcgg
     5341 gcctaccgag gacgacgtga cgcgcgacgc cgtggccggc gcgctggggc gggaactgat
     5401 cttccacgat gaaatccgcg agcggatcga gcagcggttc cggcgcatgg ggcggcggat
     5461 ggtggagatc aacaagcgcc aggcgtatgt gatcgaaggg gccgagtggc tgccgaacga
     5521 caacggcacc gcgcccgcgc aatggatcga ggattccgga cgcgtggtga tcctgctgcc
     5581 cgggccgccc agcgaactga agccgctgtt cgatcaggaa tgcctgccca ggctgaagcg
     5641 gcgcctgcca ccgctggtga tccggacgct tttcttccgt gtcgccggca tgccggagtc
     5701 ggacctggac cagctcatct cccccgtcta caagcagtac gaaaacccgg tgacgacgat
     5761 cctggcggcg ccgggcgata tccagatcca cctgcgcgcg cgctgcgaga ccgaggagga
     5821 ggcgcagcgg ctgctggcgg aggcaggcgg aaggattgcg gagcttctcg gcgaccggat
     5881 ctactcgagg aacggcgatc cgctcgaagc ggtggttggg caacggctgc gcgagcgggg
     5941 ggagacgctc agcgtggccg agagctgcac gggcgggctg ctggcggagc ggatcacgca
     6001 gattccgggc agctcggatt actttctcgg gggctttctt gtttacaccg agcggatgaa
     6061 gacggagctg cttggcgtgg atgccgctct gctgggggag catggcgcgg tgagcgaagc
     6121 ggcggcggag gcgatggcaa tcggggcgcg gaatcgcacc ggatcgacgt atgcgctgtc
     6181 cgtgacgggg tatgccggac cggacggcgg cgacgagggg gctccggtgg gaacagtgtt
     6241 catcggactg gcgtcggcat cgggctgcca ggcgcggcgg ttccgtttcc tcggcggccg
     6301 cgaccgtgtc cgcgcgatgg cggcgcagac ggggctggat ttgctgcggc ttaacagccg
     6361 tcagcgatca gcgatcagcg atcagcaaaa agctgatagc tgaaagcagc gctacccacg
     6421 tcccgccaac cgccttgatg ccgagaattt cggtgacgga agccgcggac agatggaccg
     6481 ttctcgctca cccaggccgc cagggcggcc accggaacgt ggcgctggag cacgagtgct
     6541 gccacgttgg gtgatatgat gcggaacgtc gaccgcaacc acgcgggcga agcgagcgat
     6601 ctccgtgcgg cgagtgagga gataagagga tctcttgccg gactcgttgg gaactgtccg
     6661 tctgtccctg cttttgtccg ccagttccat ctgtatggtg ggcaggcagc agcgggcgct
     6721 gacttcgcga cgatctcggc aggcgggcgc gttcagacag ttgggccagg gcgacaaatg
     6781 gtggagttac caaacggaac gaaagtgctt cgtcgcccga gcacagagca ggaggccccg
     6841 actctcgaga caaaggcgcc gcaaggccga cccatgaagt ttcgctatga ccagtaacca
     6901 gaagatccag cctgtccgcg atcgagaact gatcgacaga atgcggcagc tggccgcggg
     6961 agtggtcgtc ggtacaggtg acgtcagtcg cgaatgggtc ctgaaaaaac actgggtggt
     7021 tgtgccggtg gaaagtgacg accacttctc cggagccaca gttgcacgga tcgcggcaag
     7081 ctgtcgagac ggcaaagacc agtgcttcgc cctcattact gaggaggttt acaacatcga
     7141 ttcgtgtttc ttagttccgt gcgaggaagc ggcattggag gaacttaaca atgctctagc
     7201 tttgttcccg ttcgtgctgc ttccacagga acggtcgtgg tgtatcgtct gcactaagga
     7261 agattactat cttgtcgcgg gacccagtgg gtttgttgag acggtggtcg gcatgagcat
     7321 agctgatgca caagcggcat atcggcggtc ggcggacgaa tggatcatgg aaaagcagaa
     7381 acagtttcat cggtcagtag cagcgcggta cgaggaactg acatctgcta ctgcaagcaa
     7441 tcgggcctga aagcatatcg ttcccgccat gaattcggcg
//
LOCUS       DATIFQ010000244        10665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1610562, whole genome shotgun sequence.
ACCESSION   DATIFQ010000244 DATIFQ010000000
VERSION     DATIFQ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610562"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VLE22_11230"
     CDS             <1..1217
                     /locus_tag="VLE22_11230"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF010081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HSB15023.1"
                     /translation="DRVETCPISGTARRTGDPMHDADSIRQLLNSAKEESELTMCTDV
                     DRNDKSRVCAPGSVRVIGRRLIESYAGVFHTVDHVEGILQPGYDALDAFLSHMWAVTV
                     IGAPKKAAAQAIEDLEKDARGWYGGAVGMLSLCGDMNTGILIRTIHLRDGVAAYPVGA
                     TILYDSIPAMEEQETRLKATNFFRTLEEKRPEPEARAEAVFTSSEGGGVKLLLVDNDD
                     CFIHTLANYARQTEAEVVTYRAGFPLSLIGELAPSLVLISPGPGRPADFGVPDLVRYC
                     AQSGIPVFGVCLGLQGIVEAFGGELGVLDYPMHGKPSVVVHHGKGVFEGLPEKFTVGR
                     YHSLFARKETFPACLEVTSESEDGVIMGVRHRELPVEAVQFHPESMLTLHGDCGLRLM
                     ENVVRLYGHARV"
     gene            1238..2563
                     /gene="hpnE"
                     /locus_tag="VLE22_11235"
     CDS             1238..2563
                     /gene="hpnE"
                     /locus_tag="VLE22_11235"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HSB15024.1"
                     /translation="MSHVIIAGGGLAGLAAAAALGGEGYRVTLFESRAFLGGRATSFP
                     LSPSDEDSEVIDNCQHILLRCCRNLLDFYRRLGVDGSIRFHRQFHFLEPGGRMSVFRA
                     GLLPAPLQFTGSFRKLSFLSLPEKLAVARGLMALRREYGRRRDLDQLTMLDWLREKRQ
                     PEQAVRRFWHPVLVSAVNEELDRMAASHAFQVFRLGFLGRRDSYEMGVPDVPLGSLYS
                     SESWARLGGVEIRFRSPVERILIGGGRASGALVNGKTHAADFYVCAVPFERVLAVAPD
                     AGLDVSAFEHSPITGIHLWFDRPVTKLPHAALLDRTIQWLFNKSGGCYVQLVVSASRS
                     LIEMPRGEVIDLALRELAEFFPATREARLEKAHVVKEARATFSARPGLESRRPPAETS
                     IPNLFLAGDWTRSGWPATMEGAVRSGYLAAEAVTRVAGDPKRFLLPDVA"
     gene            2599..2976
                     /locus_tag="VLE22_11240"
     CDS             2599..2976
                     /locus_tag="VLE22_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB15025.1"
                     /translation="MRLVLALFALLAASTFPLAADPPMTKLRIEVKNHTDKPVERASV
                     IVRFVKGRSAMKLGKKVRKHWEMRTNQDGIAAIPPIPQGELTVQVIAKGYQTFGQKFD
                     VEEEEKTIEVTLNPPQPQFSAHQ"
     gene            complement(2984..3364)
                     /locus_tag="VLE22_11245"
     CDS             complement(2984..3364)
                     /locus_tag="VLE22_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSB15026.1"
                     /translation="MKTIIATDRAPKAIGPYSQAVVANGLVFASGQIPLDPTTGILIE
                     GDIAAQTERVLENLKAVLEAAGSSLDRVLRVTVFLKDVNDFAAMNDVYGRYFANQPPS
                     RSTIEAARLPRNAKVEMDAIALAG"
     gene            3549..5144
                     /locus_tag="VLE22_11250"
     CDS             3549..5144
                     /locus_tag="VLE22_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB15027.1"
                     /translation="MSKPQKFHSLAAKFSVFTAMLVIWITSLQVAYHWTQDYFSIQEH
                     VSLSILLLIFAVLLARFTSRVFVRPLVVLQRGMASVVQGRLEPVRISPTHDEIENLGD
                     SFNRMIAALAESRREVREHQEQLERKIRDRTQALEKETRRAEAASRAKSEFLANMSHE
                     LRTPMNGILGMIDIVLDSPLTDAQREELATAKDCSFSLLGLLNDILDLSKIEAGKLEL
                     ESIRFPIRPLLDDCLKSVLPRTRQKRLDLLSELSPDVPDYLLGDPLRLRQILINLLGN
                     AVKFTDRGSVKLSLTGRRLPDQQRAELHFAIADTGVGIPKQKVHAIFDEFTQVDGSTT
                     RKYGGTGLGLSITRKLVELHGGRIHVESEPGRGSVFHVLLDLVVAGPAPTEAAPNTAE
                     TVAHPHQPARILIAEDNPVNQKVAAVLLGKRGYQTTVVNHGREAIDALERAEFDLVLM
                     DLQMPVLDGIEATKLIRQDARWHDLPIIALTAHAMQGDRERCLAAGMNEYVPKPVQPP
                     VLLATVRKCLAGSLAGATKSLRA"
     gene            complement(5227..8814)
                     /locus_tag="VLE22_11255"
     CDS             complement(5227..8814)
                     /locus_tag="VLE22_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HSB15028.1"
                     /translation="MPRCWFILPYALTISIALPAVLPRKTPVNAARVRQARERATAAF
                     GRNYDARRLVFGSPQQRLAQAADPAVQRRRLVAESGVAELSAAIGDEVRVTWNESGLP
                     RTLRPVQGALSSPSGKGPRAAALDFLRSQPALFPLSGAAIESLQAVRESPIGSGTQIT
                     FQQGILGVPVFRGLIQVTVDASGQVLGIAFGDVLLNTAVDTTPQLTPQQAIGAALRRL
                     GKPVTEALVPLPASPAGRLRFRNPVKPAWSPVLVEPAVFPLSPESGHLAYRIYLQLER
                     GRHYELLVDAASGKLLFSQNLVHSAGKARVWKESPVQGPREMVDLPDSWLSPGTTVTK
                     GNNVDAYLDTDSDDEPDPGTDAELSDGRAYSATQFFVFMAGEGSTGQDPTQFGPASVT
                     NLFYLVNIAHDYFYDLGFDEIAGNFQTLNFSRGGLGNDHVLAEAQDYWASLEGGNFVT
                     LPDGESPRMEVGVDSGADPTVPTDDRDGSYSGQLVTHEYAHGVTDRLVGGPNEVTCMF
                     ASVQSVALSEGTSDYFAVSYFNNPVLDAYMSRNPDSGYRRRSYENYPLTYEHFGNEGF
                     EGHNDGELWAAALWDLRKQLGQQVTDRLVMNSFKTLPCTPTLIDARDAILSADDLTNG
                     GANRRKIWESFARHGFGNSARALGKLAGADPLVFDAAWDLPPDYVTTNRGPKVISQPP
                     EYAMYGEKLTYSIKASDPDGDKLTYELVEGPSGMTVDANTGLLQWMPTFTARRAVIRI
                     TDGKDGKVTHGILVPVFTPLEANSPIGIDGPRNSFGVAVMNVPAKTTILQFTLRGGNG
                     DPDMTVVDPLFDIYGMSFESGTTETISVATPEAGFWGVIVEASGAYQNVTLKGALLGA
                     EVLTPGIPKSGLSGDRTSETFYKVVVPEGASRLRVTTSGGTGDLDLMARFGKAPVCSG
                     ILAPCDFDEASWENGNQESVDIADVLPGEWFINLSGYEAYQDATLLVTLIGPDFSAEG
                     VTNGASFLPPVSPGGIFTVFGKKLAREEAKAGSVPLPNTLGDTSVTVNGTPAPLFYVS
                     PAQINAQIPFETKPGEASVVVKSGDMLSDTVKISVLASAPGIFLYGGDKPIVVNHEDG
                     SLNDAAHPVKAGKYIIAYLTGVSPLDNPLQTGAAVPYPPLFQCTAPFSATLGGKPAEV
                     GFLGLTPNYVGLGQANIKTPADLAPGNHALRITIGGVESNGPIVAVAAP"
     gene            9209..>10665
                     /locus_tag="VLE22_11260"
     CDS             9209..>10665
                     /locus_tag="VLE22_11260"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSB15029.1"
                     /translation="MPLAVSRGTGRHRRIGPARKMLVLAALSLPSLFGQETPAIPASV
                     AIYYDGPDQPLAEGFLDAHQIQNLLGHFGLSGEIIPIANYRPGQLARYRAAFFLGTIS
                     GAVFPKGFLADVAASNRPFCWIGRHVGSLLAAPEARGRFGFTYIDYRDDLEFRQVIYK
                     GIALPKEDPDLSIVEAGGPGVQVVAEAVNDEKTRRPYALRQGRFWYIADTPFSYAQEG
                     GRYLVFCDLLHDILEVDHPAQALALARVEDVSADIDPADVRALADTLSGLGVPFQIGV
                     IPIFRNPAKGYEIYLSDRPALAEAVRYAIERGGAPVLHGVTHQYRGVSADDYEFWDAA
                     GNRAVAGDSAEAFLRKVDLGLRELARNDIFPVAFETPHYAASEVDYRVMRDVFPLFNE
                     RTMATPDINAIQFFPYPTVDRFGRYVVPENLGYLVVESPDPRVVIERARAMRVVRDAI
                     ASFYFHAFLDPALLGEVVRGVRSAGFRFVSLRQFGG"
BASE COUNT         1920 a         3338 c         3394 g         2013 t
ORIGIN      
        1 gagaccgcgt cgagacctgc ccgatctcag gcaccgcccg aaggaccggc gatccgatgc
       61 acgatgccga cagcatccgg cagttgctga attccgccaa ggaagaatcc gagctgacga
      121 tgtgcaccga cgtggaccgc aatgacaagt cgcgggtttg tgcaccgggc tcggtaagag
      181 tcattggccg gaggctgatc gagtcctacg ccggcgtttt ccacacagtc gatcatgtgg
      241 aaggaattct tcagccaggc tacgacgcgc tggatgcatt tctcagccac atgtgggcgg
      301 ttacggtcat cggagctccg aagaaggctg cggcgcaggc gatcgaggat cttgagaagg
      361 acgctcgcgg ctggtacgga ggggcggtcg ggatgctgtc gctctgcggc gacatgaaca
      421 ccggcattct aatccggacg attcatcttc gcgacggcgt tgccgcctat cctgtaggcg
      481 cgacaatcct gtacgactcc attccggcga tggaggaaca ggagacgcgt ctcaaggcaa
      541 ccaatttctt ccgcacgctg gaagagaagc gaccggaacc cgaggcccgc gcggaggcag
      601 ttttcacgag cagcgagggc ggtggcgtaa agctgctgct ggtggacaat gacgactgct
      661 tcatccacac cctggccaac tacgcccggc agacggaggc tgaagtggtc acttaccgcg
      721 ctggatttcc gctgtcgctg atcggcgaac tggccccaag cctcgtgctg atctcgcccg
      781 gaccgggccg tcccgcagat ttcggtgtgc cggacctcgt ccgctactgc gcgcaatcag
      841 ggatcccggt tttcggtgtt tgtctcggcc ttcagggcat cgtcgaggcg tttggcggcg
      901 aactcggtgt gctcgattat ccgatgcacg gcaagccgtc ggtggtggtc catcacggaa
      961 aaggagtttt cgaggggctg cccgagaagt ttacggtcgg ccgctatcac tccctgtttg
     1021 cgcggaagga aacgttcccc gcctgcctcg aggtgacctc tgaatccgag gatggggtga
     1081 tcatgggtgt gcggcaccgc gagctgcccg tggaagcggt gcagttccat ccggagtcga
     1141 tgctcacgct tcatggcgat tgcgggctcc ggctgatgga gaacgtggtc aggctctatg
     1201 gccacgcaag ggtctgacgc gcggccgtcg taacggaatg tcccacgtaa ttatcgccgg
     1261 cggcggactg gctggtcttg cggccgccgc agcgcttggc ggagagggct accgggtcac
     1321 gctcttcgaa tcgcgggcat tcctcggggg ccgggcgacg tcgtttccgc tgtctccttc
     1381 cgacgaggat tccgaggtca ttgacaactg ccagcacatt cttcttcgct gctgtcgaaa
     1441 tctgctcgac ttctaccggc ggctcggcgt ggatggaagc atccgcttcc atcggcagtt
     1501 ccactttctg gagcccggcg gaagaatgtc ggtgtttcgc gcgggactgc tgcccgcgcc
     1561 actgcaattc acaggatcat tccgaaaact gtctttcctc agcctcccgg agaaactggc
     1621 ggtcgcgcgc gggctgatgg ccttgcggcg ggaatacggg cggcggcgcg atctcgacca
     1681 gctcacgatg ctcgactggc tgcgggagaa gcgccagccg gagcaggcgg tcaggcgctt
     1741 ctggcatccc gtgttggtga gcgccgtcaa tgaggaactg gaccgcatgg ccgccagtca
     1801 cgcgtttcag gtcttccggc tgggctttct gggaaggcgc gacagttacg aaatgggcgt
     1861 tcccgatgtg ccgctggggt cgctctactc ctcggaatcc tgggcgcgtc tgggtggggt
     1921 ggagatccgc ttccgttctc ccgtcgagcg aattctgatc ggcggcggcc gggcgtctgg
     1981 cgcccttgtc aatggaaaga cgcacgcggc cgacttctat gtatgcgccg ttccattcga
     2041 gcgagtgctg gcggtggcgc cggatgcggg actagacgtt tcggcattcg agcactctcc
     2101 gatcaccggg attcacctgt ggttcgaccg tccggtcacg aagcttccgc acgcggcgct
     2161 gctcgatcgg accattcagt ggttgttcaa caagtccggt gggtgctacg tgcaactggt
     2221 ggtcagcgcg tcgcgcagcc tgattgagat gccacggggc gaagtgatcg atctggcgct
     2281 ccgggagctg gccgaattct tccccgctac cagggaggcg cggctcgaga aggcgcacgt
     2341 ggtgaaggag gcgcgagcga cgttctcggc ccggcccgga ctggagtcgc gccggccacc
     2401 ggcggagacg tccatcccga acctgtttct ggccggagac tggactcggt ccgggtggcc
     2461 ggcgaccatg gagggcgcgg tgcgcagcgg gtatctcgcc gccgaagccg taacccgcgt
     2521 ggccggcgat ccgaaaaggt tcctgttgcc ggacgtggcg tgaacatccc ccgcccggtc
     2581 ttttgggata atcgaactat gcgtcttgtg ctggctctgt tcgcgctttt ggcggcgtcg
     2641 acgtttccgc tcgcggccga cccgcccatg accaagcttc gcattgaggt aaaaaaccac
     2701 accgataaac cagtggagcg ggcgagcgtg atcgtccgat tcgtcaaggg ccgttcggcg
     2761 atgaagttgg gcaagaaggt ccgaaagcat tgggagatgc gcaccaacca ggacggcatc
     2821 gccgcgatcc cgccgatccc gcagggggaa ctcaccgttc aggtgattgc caagggctac
     2881 cagacgttcg gccagaagtt cgacgtcgag gaagaagaga agacgatcga agtgacgctg
     2941 aacccgccgc agccgcagtt ctcggcccac cagtagccgg cggtcagccc gccagagcga
     3001 tcgcatccat ctccaccttt gcgttgcgcg gaagacgcgc ggcctcgatc gtagaccggg
     3061 agggtggctg gtttgcgaag taccgcccgt atacatcgtt catcgcggcg aagtcgttga
     3121 catctttcag aaacacggtc actcggagga ccctgtcgag cgaggaaccc gcggcctcga
     3181 gcacggcttt caagttctcc agaacgcgct ccgtctgcgc ggcgatgtct ccttctatga
     3241 ggattccggt tgtcgggtcg aggggaatct gaccgctggc aaaaaccagc ccgttggcga
     3301 cgacggcctg cgagtaaggg ccgatcgcct tcggtgcgcg gtccgtcgcg atgatggtct
     3361 tcatttcgtt tcctccagag agcggctcgc ggtttgatca cgcgcggcgc acactccatt
     3421 ctgcacgccc gccggtcccg agcgccaggg caacccgata aggcggaatt cagccggtgc
     3481 tcagttgttc gactgatggc cagctacccg caaaggcccg accataaacg tgaggtagtc
     3541 cgtcgttcgt gtcgaagccg cagaaattcc actcgctggc cgcgaagttt tctgttttca
     3601 ccgcgatgtt ggtgatctgg atcacttctc tccaggttgc ctaccactgg acgcaggact
     3661 acttctcaat ccaggaacac gtttctctca gcattctgct gctcatcttc gcggtcctgc
     3721 tggcgcgttt cacaagcaga gtgttcgttc ggcctcttgt cgtgctccaa cgaggcatgg
     3781 cgtcagtcgt ccagggcagg ctggagccgg tgcggatttc gccgacgcac gacgagatcg
     3841 agaaccttgg cgacagcttc aaccggatga tcgcggcgct ggccgagtcg cgacgggaag
     3901 tgcgcgagca tcaggaacag cttgagcgaa aaatccgcga ccgtacccaa gcgctcgaga
     3961 aggagacgcg gcgcgcggag gcggcgagcc gggcaaagag cgagtttctc gccaacatgt
     4021 cgcacgagct gcggaccccg atgaacggca tcctcggaat gatcgacatc gtgctcgata
     4081 gcccgctgac ggacgcgcaa cgtgaggaac tggccactgc caaggactgc tctttctcgc
     4141 tactcggcct gctcaacgac atcctggacc tctcgaagat cgaggccggc aagctggaat
     4201 tggaatcgat ccgtttcccc atccggccgt tgctggacga ttgtctgaaa tcggtcctgc
     4261 cgcggacccg tcagaagcgg ctggatctgc tgtcggaact ttcccccgac gtgccggact
     4321 acctgctcgg cgatccgctc aggttgcgcc agatcctgat caacctgctc ggcaacgcgg
     4381 tcaaattcac ggatcgcgga tcggtgaagc tttccctgac gggccgtcgc ctgccggacc
     4441 agcagcgcgc ggaactgcat ttcgcgatcg cggataccgg cgtcggcatc ccaaagcaga
     4501 aggtccacgc gatcttcgat gaattcacgc aggtggacgg cagcaccacg cggaagtacg
     4561 gtggcacggg actggggctg tcgattacga gaaagcttgt cgagttgcat ggtggccgta
     4621 tccatgttga gagcgagcct ggccggggca gcgtgttcca cgtcttgctg gatttggtgg
     4681 tagcgggacc ggcgcccacc gaagcggcgc cgaatacagc ggagaccgtg gcgcacccgc
     4741 atcagccggc gcgaattctc attgcggagg acaatcccgt caaccaaaag gttgctgcgg
     4801 tcctgctagg caagcgtggt taccagacga ccgtcgtgaa tcacgggcgc gaagccattg
     4861 acgcactgga gcgcgcggag ttcgacctcg ttctaatgga tctccagatg ccggttctcg
     4921 acggcatcga ggcgaccaag ctgatccgcc aggacgcgcg ctggcacgac ctgccgatca
     4981 ttgccctcac cgctcatgcg atgcaggggg atcgggaacg ctgtctcgcc gccggcatga
     5041 acgagtacgt cccgaagccg gtacagccgc cggtgctgct tgcgaccgtg cgcaaatgtc
     5101 tcgccggcag cctcgccgga gcgacgaagt cgctccgcgc ctagcagtgg cgcgggccat
     5161 cgtcttctgt ggcctgcggg ggcggcagac gacaaaagct gatcgtctgc cccatatcaa
     5221 cccgcatcac ggtgccgcga cggcgacgat cgggccgttg ctctcgacac ctccgatcgt
     5281 gatccgcaac gcgtggttgc ccggcgccag gtccgcgggc gtcttgatgt tcgcctggcc
     5341 gagaccgacg taattgggcg tcaaaccgag gaagcccact tccgcaggct ttcctcccag
     5401 tgtcgcgctg aatggtgcgg tgcactggaa cagcggcggg tacggaaccg cggcgccggt
     5461 ctggagcgga ttatcgagtg ggctcacgcc ggtcaggtac gcgatgatgt acttacccgc
     5521 cttgaccgga tgcgcggcgt cgttaagact gccgtcctcg tgattgacga cgatcggctt
     5581 atcgcctccg tagagaaaga tcccgggagc cgaggccaga accgagattt tcaccgtgtc
     5641 gctcagcatg tctcccgact tgactaccac cgaggcctcg cccggcttcg tttcgaaggg
     5701 gatctgcgca ttgatctgcg cggggctgac atagaataac ggagcaggcg tcccattgac
     5761 cgtcaccgag gtgtcgccga gcgtatttgg caacggcacc gatcccgcct tcgcctcctc
     5821 cctggccaat ttcttgccga agacggtgaa gatcccaccc ggtgagaccg gcggcaggaa
     5881 cgaagcgccg ttggtgacgc cctccgcgct gaagtctggt ccgataaggg taacgagtag
     5941 agtggcgtcc tgataggcct cgtacccgct cagattgatg aaccactcac cgggcaagac
     6001 gtcggcgata tccaccgatt cttgattccc gttttcccaa ctggcctcat cgaagtcgca
     6061 cggagcgagg ataccggagc agaccggcgc tttgccgaaa cgggccatca ggtcgagatc
     6121 gcccgttcca ccgctggtcg tcacccgcaa acggctggcg ccttcgggta cgacgacctt
     6181 gtagaaagtc tccgaagtcc ggtcgcccga aagcccggac ttcgggatcc ccggagtcag
     6241 aacctcggcg cccagtaacg cccctttcaa ggtcacgttt tggtatgccc cactggcctc
     6301 cacgatcacg ccccagaagc ctgcttccgg cgtggcaacc gagatggttt ccgtcgtccc
     6361 ggattcgaac gacatcccgt aaatgtcgaa taacggatcg accaccgtca tgtcgggatc
     6421 tccgttcccg ccccgcaggg tgaactggag gatggtggtc tttgccggca cgttcatcac
     6481 ggcgacccca aagctgttcc tcggaccatc gatgccgatc ggactgttcg cctcgagcgg
     6541 tgtgaagacc gggaccagaa tgccgtgcgt gaccttgccg tctttgccgt ccgtgatgcg
     6601 aataacggca cgccgggccg tgaaggtcgg catccactgg agcagacccg tgttggcatc
     6661 gaccgtcatg ccggaaggac cctcgaccag ttcgtacgtc agtttgtcgc catccgggtc
     6721 ggacgccttg atggagtaag tgagtttctc gccatacatc gcgtactcgg ggggctggct
     6781 gatgaccttg ggaccgcggt tcgtggtgac gtagtcgggt ggaaggtccc aggcggcgtc
     6841 gaaaaccagg ggatccgctc cggcgagttt gccgagagcg cgggcggaat tgccaaaacc
     6901 gtgccgggcg aacgactccc aaatcttgcg gcggttcgca cctccgttgg tgaggtcgtc
     6961 cgcgctcagg atggcgtccc gcgcatcgat cagggtgggg gtgcagggca atgtcttgaa
     7021 gctgttcatt accagcctgt cagtgacctg ctggccgagc tgctttctca gatcccataa
     7081 cgcggctgcc caaagttctc cgtcgttgtg tccctcaaag ccctcgttgc cgaaatgctc
     7141 gtaggtgagc gggtagttct cataactgcg ccggcggtaa cccgaatccg ggtttcggct
     7201 catgtacgcg tcgagaaccg ggttattgaa gtagctcacc gcgaagtaat cggaggtccc
     7261 ttcggataac gcgaccgact ggaccgaggc gaacatgcaa gtaacttcgt ttggccctcc
     7321 cacgaggcgg tccgtgactc catgtgcgta ctcgtgggtg acaagctggc cactgtagga
     7381 gccgtcccgg tcgtccgtgg gcaccgtcgg gtcagcgccg gagtcgacgc caacctccat
     7441 tctgggagac tctccgtcgg gcagcgttac gaagttgcct ccttcgagtg atgcccaata
     7501 gtcttgcgcc tccgccagca cgtggtcgtt ccccaacccc ccacggctga agttgagcgt
     7561 ctgaaagttt ccggcgatct cgtcgaagcc aagatcgtag aaatagtcat gagcgatgtt
     7621 gaccaaataa aagagattcg tgaccgaggc gggcccgaac tgcgtcgggt cctgtcctgt
     7681 ggatccctcg cccgccatga acacgaagaa ctgcgtggcg ctgtacgccc tgccgtccga
     7741 caactcggca tcggttccgg gatcgggctc gtcatcggag tccgtgtcga gataggcgtc
     7801 gacgttgtta ccctttgtga ctgtcgtgcc aggactcagc cacgagtcgg gtaagtcaac
     7861 catttcccgc ggaccttgca ccggcgattc cttccagacg cgggccttgc ctgccgagtg
     7921 gacgagattc tgcgagaaca gaagctttcc ggaagcggcg tcaacgagca attcgtagtg
     7981 gcggccccgt tcgagctgca gataaatgcg ataggcgagg tgtccggatt ccggggacag
     8041 cgggaaaacg gcgggctcca cgagaacggg agaccatgcg ggcttcacgg gattgcggaa
     8101 gcgaagccgg ccggccggtg aggccggcag aggcacgagg gcctccgtaa caggcttgcc
     8161 gaggcggcgg agcgccgctc cgatggcctg ctgcggtgtg agctgcgggg tagtgtccac
     8221 ggcggtgttg aggagtacat ctccaaatgc gatcccgagc acctgccctg atgcatcgac
     8281 agttacctgg atcagaccgc ggaacacagg cactccaagg atgccttgct gaaacgttat
     8341 ctgcgtaccc gaaccaatcg ggctttcccg caccgcctgc aagctctcaa tcgcggctcc
     8401 gctcaaagga aacaacgcag gctgagaacg caggaagtcg agagcggctg ccctcggtcc
     8461 cttcccggag ggcgaggaca gcgcaccctg aaccggccga agcgtgcggg gtagtccgga
     8521 ttcgttccac gtgacgcgga cctcgtcgcc aatggcggct gagagttctg ctacaccgga
     8581 ctcggcgacg aggcgtcgcc gttggaccgc agggtcggca gcctgtgcga gccgctgttg
     8641 cggcgacccg aagaccagtc gacgtgcgtc atagttacgg ccaaacgcgg ccgtggcgcg
     8701 ctcacgtgcc tgccggacgc gagccgcgtt caccggcgtc ttccgcggga ggacagcagg
     8761 aagcgcgatg gaaatagtca gagcgtatgg aagaatgaac caacaacgag gcatcggcgg
     8821 tctccctcaa acttcgatgg accgccaggg gattcgggca accggagccc actgcggcgg
     8881 ggagctccac atccgcgatc gcccatcggg gatgcatcca ctggcgactt gacagatccg
     8941 agatcactat atcaccagac tttcagagtc caaacacctt tttagagccg ccagcccgcc
     9001 cagcgagaat cccaccaccg cgcccgtaca cccacccgga agcccttcgc ttcgagctgt
     9061 cgagttttct gctccgtgcg ggcgacgccc gtcacctcgt gccctttgga gcgagtctag
     9121 caggatttat cggctaacct gtagaaatct ctaggcgttt ctgtcaaagc ggtgtacaat
     9181 acatggaacc ggtgccgaca ggctcactat gcctctggcc gtttctcgag ggacgggacg
     9241 acaccggcgc atcgggccgg cccgcaagat gctggtgctg gccgctttaa gtctgccttc
     9301 tctcttcggc caggaaacac ccgcgatccc cgcctccgtt gcgatctact acgacgggcc
     9361 ggaccagccg ctggccgagg gcttcctcga cgcccaccag atccagaacc tgctgggcca
     9421 cttcgggctc tccggcgaga tcatccccat cgcgaactac cgcccggggc agctcgcccg
     9481 ctaccgcgcc gctttcttcc tcggcaccat cagcggggcc gtcttcccga agggctttct
     9541 ggccgatgtc gccgcctcaa accgtccgtt ctgctggatc ggccgccacg tgggcagcct
     9601 gctcgctgcc cccgaggcgc gtggccgctt cgggttcacc tacatcgact accgggacga
     9661 cctcgaattc cgccaggtca tctacaaagg gatcgcgctt ccgaaagaag atccggatct
     9721 gagcatcgtc gaggccggcg ggccgggcgt ccaggtcgtg gcggaggcgg tcaacgacga
     9781 gaaaacccgg cgcccctacg cgctccggca gggccgcttc tggtacatcg ccgatacgcc
     9841 gttttcctac gcccaggaag gcgggcgcta tctcgtcttc tgcgacctgc tgcacgacat
     9901 ccttgaagtg gaccaccccg cgcaggcgct ggctctggcg cgggtggagg atgtcagcgc
     9961 cgacatcgac ccggcggacg tgagagctct cgccgacaca ctatccggcc tgggtgtgcc
    10021 cttccagatc ggtgtcatcc ccatcttccg aaacccggcg aagggctacg agatctacct
    10081 ctcggatcgg cccgcgctcg ccgaagccgt gcgctatgcc atagagcgcg gcggcgcccc
    10141 cgtgctgcac ggcgtcaccc accagtaccg cggagtcagc gctgacgact acgagttctg
    10201 ggacgcggcg ggcaaccgtg cggtggccgg ggactcggcg gaggcgttcc ttcgtaaggt
    10261 cgatctcggt ctgcgcgaac tggcgcggaa cgacatcttc ccggtggcct tcgaaactcc
    10321 acactacgct gcctcggaag tggactaccg cgtcatgcgg gatgttttcc cgctcttcaa
    10381 cgaacgcacg atggcgacgc cggatatcaa cgccatccaa tttttcccct atccgacggt
    10441 ggaccgcttc ggccggtacg ttgtgccgga gaacctcggc tacctcgtgg tcgaaagccc
    10501 cgacccacgg gtggtcatcg aacgcgcccg cgcgatgcgc gtggtgcgcg acgcgatcgc
    10561 gtcgttctac ttccacgcgt tcctcgatcc ggcgctgctt ggcgaggtcg tgcgcggagt
    10621 ccggtcggcg ggctttcgct ttgtctcgct gcggcagttc ggcgg
//
LOCUS       DATIFQ010000245        11112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1037447, whole genome shotgun sequence.
ACCESSION   DATIFQ010000245 DATIFQ010000000
VERSION     DATIFQ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037447"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VLE22_11265"
     CDS             <1..414
                     /locus_tag="VLE22_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HSB15030.1"
                     /translation="TQPPAGRPGAIRRLLFQDLRYKFAALGMAAAVIALSVLSTGSTV
                     RAISVPVEFVNVPPGMAISSQSPSRIEAQVRGSRWLIDSVTDSGLVARVDLRRAGQGV
                     QDVRVRPDALNVPPGVVVERVTPPGITVRLVRKLP"
     gene            453..2318
                     /locus_tag="VLE22_11270"
     CDS             453..2318
                     /locus_tag="VLE22_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HSB15031.1"
                     /translation="MKLSVQVLASLFASLSSAQTPCQPTAVYTPCDIVFDMTDEEARA
                     HPNPYLTVQLQAEFRSPRYRTFLMPAFWDGGRRLVIRFAPTDPGKWDFRISSNIARFD
                     EKTGQVEATPSDSPGFVRPRNVHHWGYTETDQPHLWMGDTLYSFATVDQAVFEKVIEA
                     RAQQKFNHIRGLVLGREQDAAKAFPSPEQPDPEHFRRLDGRILAMNRKGIVADLIIGG
                     DQNELAKLFPTWQQRERYIRYLVARYSAMHITWQGVQEFEEYEDGRALLKEIGESLKK
                     LDPYNHPRSTHTVATSAPLFGDGWMSYIVYQSSDNHLGSIEHQLFAAPFVNAEFAYED
                     TGAGKSHPHHVDTDTFRKRLWNATMSGQYPTFGNTGTYGGRKFAVDPKYLDSPGAKQM
                     TVWYDFFSGTRFWELEPYFDVDGGRALALERPHDEEMEGIEFIVYVEKPGPVEIVLPR
                     HSYDAAWFNPITGERIKLKEFKDRRYRAEPPSQDHDWVLHISREGRKRGMLNSYKFES
                     RAIMLQEVEQSSQKVPYQIEEPSADSLPVGKPVKYSAKVTRDTRATRSMMWLWTGEVA
                     AGGQGYRVLGTGADGEMKIPPGLAKRYPAVLNLRLAGMNAVGKVYFSDKVYRLTE"
     gene            2315..3007
                     /gene="tsaB"
                     /locus_tag="VLE22_11275"
     CDS             2315..3007
                     /gene="tsaB"
                     /locus_tag="VLE22_11275"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HSB15032.1"
                     /translation="MTSRILAVDTTTEFGGLALLYGSEVVEELLLHSSEGFGHILFAN
                     VERLLAKHAWAVLDIDCFAAAAGPGTFTGTRVGLAAVKGFAEAAGKPVVAVSNLAAVA
                     SFGSAPLRAAVLDARRGEVYGAVYSGSLELICPEIVTKFPAWLESLPAGDLEFLSPDF
                     APFRHALAGTRFENAPVRQTPRSLAAAVGAIAVRRFIAGSFEDPATVDANYVRHSDAE
                     LLWKDRQATENQ"
     gene            complement(3042..4184)
                     /locus_tag="VLE22_11280"
     CDS             complement(3042..4184)
                     /locus_tag="VLE22_11280"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HSB15033.1"
                     /translation="MSRTSSTRADRRGFLQRAMALAGFTAAVPAAASAQAADESSRRF
                     LPRYARAQDYRSLKQSSHDITGGNRDYWPIAPGATQEVFSQEGPGVITHIWFTIAAQG
                     IHHLKEIVLRIYWDGEGKPSVEAPVGDFFGLNLGQYVHYESAYLACSPGRSLNCYFAM
                     PYRKSARITVTNEGTQGVGAFYSNIDFQRVPALLPDALYFHAQYRQASPCVRSTGDAA
                     KLNPDGSLNYVYAETRGRGHLMGVTLGVLINADGWMGEGDDMIFVDDNSRPVIIGTGS
                     EDYFLGSWNFGGRDGAVPFAHHYYGAPLIIMPERTGGRYCCYRWHGDNPVTFNRYMKH
                     TMEHGHANDRGDNFFSVCYWYQDAPYTEFPALPSAEERIPRVKLPG"
     gene            4417..5154
                     /locus_tag="VLE22_11285"
     CDS             4417..5154
                     /locus_tag="VLE22_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB15034.1"
                     /translation="MSNPGAPIQDETVPESAEEAVPSTIRVVISDDHPIVREGLRRLL
                     ALEPDISVVGEAGDGESTLTIVDQVNPDILLLDLRMPGIDGLSALQRLQRVNKRTRVI
                     VLTASEDKNEFVQAMKLGCSGIVLKQTAAELIVKSIRKVHAGEIWLDSHTTAAVMRQF
                     ASPNDPVPQGSARPRDRSPLSQREREIVALVAQGYKNKEMAEKMFISEQTVKNHLHNI
                     FDKLGVSDRLELALYAIHKGLHTEEAS"
     gene            complement(5151..6611)
                     /locus_tag="VLE22_11290"
     CDS             complement(5151..6611)
                     /locus_tag="VLE22_11290"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15035.1"
                     /translation="MNRRDVLRMLSAAPAAAQAASTATSVKPGAFAAREKQKMVLIGA
                     GSAMFTQGIVIDWLQRRPKGDWEIALVDINPVILEATEKLVRRYMMTSDQPAKVTAST
                     ERRDVLAGATVVISTIGVGSRRAWEQDVFVPRKFGVYQPVGDSVMSGGVSRAQRMIPP
                     MVAIAKDVERLCPYAIFINYSNPMTAVVRALRRTTSLPAFGLCMGTEEGIEVLAAMAG
                     APRNRVTARWVGLNHLTWITDLRLDGEDLWPVIRTKVAERRAKGLDRDSWASSFGHAP
                     DTGKLAYPFAWELFDEFNAFPAPMDRHVTEFFPERFPGGKYYGSVLGVDAYSFEGTIA
                     RGDKIYDDTLSLAQGTGPIDSARIKSTGGEHMQTMQILDSFWHDRRRWYSANLPNHGI
                     VSNLPKDVILEVPALAAQDGMLAPPIGEIPVPIAAVLLRRIAAVEATVEAALTGNRKL
                     FVEALILDGGVSDYATASKMADALIQAHAKHLPQFS"
     gene            complement(6608..6853)
                     /locus_tag="VLE22_11295"
     CDS             complement(6608..6853)
                     /locus_tag="VLE22_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15036.1"
                     /translation="MRPAFQVRVECYAGYKADERPLRFYIEDRQFDVVELLDRWYGPG
                     DTWFRVRADDDNLYVLRHTETDTWTLESFRRSTREKL"
     gene            complement(6860..8665)
                     /locus_tag="VLE22_11300"
     CDS             complement(6860..8665)
                     /locus_tag="VLE22_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15037.1"
                     /translation="MVGRITADAPASITVLDDSDIRATPGVNLDDRLRDVPGFSLFRR
                     SSSVVAHPTTQGVSLRGIGSTGASRTLVLWDGFPVNDPFGGWVQWSRFAPDDIDRIEI
                     SRGASTSVFGDRAMGGAIALFSRQPEPLHLTASYSGGNAGTHEAGAGFTHLGSRWSAS
                     LHGRTFATDGYYVVPEGIRGTADRRAGADFVAASSRLDFFGAASRLFLKFDMLAERRR
                     NGTSIQQNSTSLGSLSAHYSLARGADTFSVLASHTREEFRSAFSAIAADRNSERPTFR
                     QSVPTESVGGAVYWSRNGSRWDVLAGADVNHVEGYSKDTLFPAGLRVGGGTLLQHGTF
                     VQTDAGAGPVKFHLGARHDFTGRDRQFFSPSAGVSAGRGRLRVRTSAYRSFRAPTLNE
                     LFREFRVGNAVTRANDALRSERLFGAEAGIDYAGERSHMSFTLYRNSLSDLITNVTLT
                     SSPSLIVRQRRNAASALARGVEMHADHRWRNWFGDASYLYADSRFSTGERLPQVPSHQ
                     GSARLGWQRGATFVSAGLRSYSLQFEDELNRFLLPGFGAVQVAVRRQINARLSAFASV
                     ENLFDREYLTGFTPTPTVGAPRLWRLGLRWEGRVR"
     gene            complement(8643..9785)
                     /locus_tag="VLE22_11305"
     CDS             complement(8643..9785)
                     /locus_tag="VLE22_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSB15038.1"
                     /translation="MPLSALRSGFQKFLRYSAQRRTTPIGLVFAVAALLVAFTAVVSA
                     NNLLFLILAAMISILMISNFICRLSLAGLELDFHLPEHLSARRTFPARIFVRNGKRWM
                     PSFSVEVAGVGESAFSTPLYFPVLPGGSTLEETVEVRFDRRGLHREDGFEFSTSFPFG
                     FLERRANVTLRRDILVYPCLDPQPGFEELLDTLSGEIVAHYRGRGHDFYRIRPYVAFE
                     SARHVDWKATAHTNELQVREFAREEEPLVEILLDLDVPDAHREWFDRAVDCCAYTTWH
                     VVRREARVCFSTQDFRLTVPATGDIYTILKYLAMVSPKASKPPAGPHEENSFQIVFSA
                     SPERYPVARWRSACVVGPDLLPAPPPRRDATRAGQEFDHGGRTDHR"
     gene            9815..>11112
                     /locus_tag="VLE22_11310"
     CDS             9815..>11112
                     /locus_tag="VLE22_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HSB15039.1"
                     /translation="MSTEAPTAAPDGGPRLKRALSLWDLVFTGIILIQPTAPMPLFGI
                     VYQEARGHVVTTILIAMVAMLFTAISYGRMARAYPSAGSAFVYVGREIHPSIGYASGW
                     SMAMDYMLNPVLCTVWCSKAAGNILPEVPYYAWVVFFALLFTVLNLRGIKSSARTNEW
                     LAAGMGVVILIVLAAAVRYVLRLCPQETEFFTRPFYDPETFSIKAVFTGTSIAALTYI
                     GFDGISTLSEEVQNPRRNILLATVLTCLITGFLASIEVYAGQLIWPAGQAFPDADTAY
                     VHVAGRAGGLWLFQLVNLTLLVATIGSGTGSQLAAARLLYGMGRDNAIPKRFFGAIEP
                     KRGIPRNNVLFTGGLALCGAFVLSYQLTAELLNFGAFIAFMGVNLAAFTRYYLRANEK
                     KLINAVPPLVGFTICLYIWLNLRTPAMIAGAVWLAIGIVYG"
BASE COUNT         2194 a         3401 c         3470 g         2047 t
ORIGIN      
        1 acccaaccgc ccgcggggcg gcccggggcc attcgacggc tactatttca ggacttacgc
       61 tacaagttcg ccgctctggg aatggccgcg gcggtcatcg cgctgtcggt tctctcgacg
      121 ggctcaacgg tacgggcgat cagtgttccg gtcgagtttg tgaacgttcc gccgggaatg
      181 gcgatctcga gccagtcgcc gagtcgcatt gaagcccagg tgaggggtag ccggtggctg
      241 atcgactcgg tgaccgattc gggtctggtg gcgcgggtcg acctgcggcg ggccggccag
      301 ggcgtgcagg atgtccgggt tcgccccgac gcgttgaacg ttccacccgg cgttgtggtt
      361 gagcgagtta cacctcccgg catcacggtc cgccttgtgc gaaagctgcc ttaaagacgc
      421 cgtggagatg ctgctcccgc tacactgttc tcatgaagct gtctgtccag gttctggcgt
      481 ccctgttcgc ctcactcagt tcggcccaga ctccgtgtca gccgacggcg gtctacacgc
      541 cctgcgacat cgtgttcgac atgacggatg aagaggctcg ggcgcatccg aacccgtacc
      601 tgacagtcca gctccaggcc gagtttcgct cgccgcgtta tcgcaccttc ctaatgccgg
      661 cgttctggga tggcggccgg cgcctggtga tccggttcgc gccgactgac ccgggcaagt
      721 gggacttccg catttccagc aacatcgccc gatttgatga gaagaccgga caggtggaag
      781 ccacgccctc ggactcgccg ggcttcgtcc ggccccgcaa tgtgcaccat tggggctaca
      841 cggagaccga tcaaccgcac ctgtggatgg gcgacacact ttacagtttc gcgaccgtgg
      901 atcaggcggt attcgaaaag gtcatcgagg caagggcgca gcagaagttc aaccacatcc
      961 gtggccttgt gctcgggcgg gagcaggacg ccgcgaaggc ctttccttca ccggaacagc
     1021 ctgatccgga gcacttccgc cggctggacg gacgcatcct tgcgatgaat cgcaaaggaa
     1081 tcgtcgccga tctgatcatc ggtggcgacc agaacgaact agcgaagctg ttccccacct
     1141 ggcagcagcg cgaacgctac atacggtacc ttgttgcacg ttactcggcg atgcacatca
     1201 cctggcaggg cgtgcaggaa ttcgaggagt acgaagatgg gcgagcgctg ctcaaggaaa
     1261 tcggcgaatc cctgaagaag ctcgacccat ataaccatcc gcgttcgacg cacacggtag
     1321 cgacgtcggc gccgctgttc ggcgacggct ggatgagtta catcgtgtat cagtcctcgg
     1381 acaaccatct cggttcgatc gagcaccagc tcttcgccgc accgttcgtc aatgcggagt
     1441 tcgcgtacga ggatacgggc gcgggcaagt cgcatccgca tcacgtcgac acagacacct
     1501 ttcgcaaacg cctctggaac gcgacgatga gcgggcaata tccgacgttt ggaaacaccg
     1561 gcacctacgg aggcagaaag ttcgcggtag atccgaagta cctcgactcg cccggagcca
     1621 agcagatgac ggtctggtac gacttcttct ctggaacgcg attctgggag ctggaaccct
     1681 attttgacgt ggacggtggc cgcgctctgg cgctggagcg tcctcacgat gaggagatgg
     1741 aaggcatcga attcatcgtc tacgtggaaa agccgggacc cgtggagatc gtgcttcccc
     1801 gccacagcta cgacgccgcg tggttcaatc cgatcaccgg cgaacggatc aaactcaagg
     1861 agttcaaaga caggcgctac cgcgccgagc cgccaagcca ggaccacgac tgggtgttgc
     1921 acatctcgcg ggaaggccgg aaacgcggca tgctgaattc gtacaagttc gagtccaggg
     1981 cgatcatgtt gcaggaagtg gaacagagtt cgcagaaggt cccgtatcag atcgaagagc
     2041 catcggcgga ctccctgccg gtgggcaagc cggtcaagta ctccgccaag gtcacgcgcg
     2101 acacccgggc gacgcgctcg atgatgtggc tatggacagg cgaggttgcc gcgggaggtc
     2161 agggctatcg ggtgctcggg actggagctg acggcgagat gaagatacct ccgggcctgg
     2221 cgaagcggta tccggccgtg ctcaacctcc ggctcgccgg gatgaatgcc gttggaaagg
     2281 tctacttttc cgacaaggtc tacaggctca ccgagtgaca tcccgaatcc tcgcggttga
     2341 cacaacgaca gagtttggcg gcctcgcgct actttacgga agcgaagtcg tcgaggaact
     2401 cctgctgcat tcgagcgagg gcttcggcca catactgttc gcgaacgtcg agcggctgct
     2461 ggctaagcac gcctgggcgg tgctcgatat cgactgtttc gccgccgccg cagggcccgg
     2521 caccttcacc ggcacccggg tgggactggc ggcagtgaaa gggtttgcgg aggccgcggg
     2581 gaagcccgtc gttgcggtgt cgaacttggc ggcggtggcg tcgttcggaa gtgcgccgtt
     2641 gcgggccgcc gttctggacg cgcgtcgcgg tgaggtctac ggcgctgtct attcgggctc
     2701 gttagaactg atctgcccgg aaatcgtaac gaagtttccg gcgtggctcg aatcgcttcc
     2761 cgccggcgac ctcgaattcc tttctccgga cttcgccccg ttccggcatg ccctggcggg
     2821 aacccggttc gagaatgcgc cggtccgcca gactccgcgg tccctcgccg ccgccgtcgg
     2881 tgcgattgcg gttcgtcgct tcatcgcggg gagcttcgag gatccagcca cggtcgacgc
     2941 caattacgtt cgccactccg acgcggagct gctctggaaa gaccggcagg ccactgagaa
     3001 tcaataactc atgtcctcat ccccgatgcc gcggatttct cctaccccgg cagtttcacg
     3061 cgggggatgc gctcctccgc agagggtaac gccgggaatt cggtgtaagg cgcgtcctga
     3121 taccaatagc agaccgagaa gaaattgtcg ccgcggtcgt tggcgtgccc gtgctccatc
     3181 gtgtgcttca tgtagcggtt gaaagtgact ggattgtcgc catgccagcg atagcagcag
     3241 taccggccgc cagtccgctc tggcatgatg atcagcggcg ctccgtaata gtgatgagca
     3301 aacggcaccg cgccgtcccg gccgccgaag ttccaactgc cgaggaaata gtcttccgag
     3361 cctgtcccga tgatcaccgg acggctgttg tcgtcgacga agatcatgtc gtcgccttcg
     3421 cccatccagc cgtcagcatt gatcaaaacc ccgagagtga cgcccatcag gtgaccacgc
     3481 ccacgcgtct cggcataaac gtagttcagg ctgccgtcgg gattcagttt cgccgcgtcg
     3541 ccggtcgaac gcacgcacgg tgacgcctgg cgatattgcg cgtggaaata gagggcgtcc
     3601 gggaggagcg ccgggacacg ctgaaaatcg atattcgagt aaaacgcccc cacgccctgc
     3661 gttccttcgt tggtaacggt aatgcgggcc gatttccggt acggcattgc aaagtaacag
     3721 ttcaaagagc gcccggggga gcaggcgaga taggcggact cgtaatgaac gtactggccc
     3781 aggttgagcc cgaaaaagtc tccgacagga gcctcgacgc tgggctttcc ctcgccgtcc
     3841 cagtagatcc ggagcacaat ctctttcaga tggtggatcc cttgcgccgc aatcgtgaac
     3901 catatgtgag tgatcacgcc gggaccttcc tggctgaaaa cctcctgtgt ggcgccgggc
     3961 gcgattggcc agtaatctcg gttaccgccg gtgatgtcgt gactcgactg cttcagggac
     4021 cggtagtcct gcgcacgggc atagcgtgga aggaaacggc gggaggattc atcggcggcc
     4081 tgcgctgaag cggcggccgg gacggctgcg gtgaaaccag ccaaggccat ggcgcgctga
     4141 agaaaaccgc gccggtccgc gcgggtagac gacgttctgg acataacgtg tgctcctttc
     4201 gagaaatcgg catgagaccg agagccggat tatatcgaaa ctggccctca agttgtgaac
     4261 agtttgatgc gttccagcag gacacagagc tgaagaatag aaaaagtttg aatttgtttg
     4321 aattttaagc acgcatcgta aggaaattcg tctttggttg agatggatgg gttgactcag
     4381 tactagaatt agccgtacaa tcgtcctaat caggctatga gcaaccctgg cgcccccatc
     4441 caagacgaga ccgtgccaga atccgccgaa gaggccgtac cttccacgat ccgagttgtc
     4501 atttccgacg accatccgat tgttcgcgag ggtttgcgga gactgctagc cctcgaaccc
     4561 gatatcagcg tcgtcggtga agcgggtgat ggggagtcca cgctgacgat cgtggaccag
     4621 gtcaatccgg acattttgct cctcgacctc cggatgccag ggatcgacgg tctgtctgcg
     4681 ctccagcggt tgcagagggt caacaaacga accagagtca tcgtgctcac agcctccgag
     4741 gacaaaaacg agttcgtgca ggcgatgaag ctaggttgct ccgggatcgt gctaaagcaa
     4801 acggccgcgg aactgatcgt caagagcatc cgcaaagtgc acgccggcga gatctggctg
     4861 gattcgcaca ccactgccgc ggtgatgcgg cagttcgcct cgcccaacga tccggtgcct
     4921 caaggctcgg cccgcccgcg cgaccgttcg ccgctgagcc agcgcgagcg cgagatcgtg
     4981 gcgctggtgg cgcagggata caagaacaag gaaatggccg agaagatgtt catcagtgag
     5041 cagacggtga agaaccatct gcataacatc ttcgacaagc tcggcgtttc ggatcgcctc
     5101 gaactcgccc tctacgccat tcacaagggc ttgcacacgg aagaagccag ctagctaaac
     5161 tgaggcaagt gcttcgcgtg ggcttggatg agggcgtccg ccatcttgga agcggtggcg
     5221 taatcgctca cgccgccatc cagaatgagc gcctccacga acagcttccg gttcccggtg
     5281 agcgcggctt ccaccgtggc ctccaccgcg gcgatccggc gaagcaaaac tgcggcgatc
     5341 ggcaccggga tctcgccgat aggcggcgcc agcatgccgt cctgggcggc caacgcgggc
     5401 acttcgagaa tgacgtcctt aggcaggttg ctgacgatcc cgtgattcgg cagattcgcc
     5461 gaataccatc gccggcggtc gtgccagaag ctgtcgagga tctgcatggt ctgcatgtgc
     5521 tcgccgcctg tagacttgat ccgagccgag tcaatcggtc ccgtgccctg cgccagactg
     5581 agcgtgtcgt cgtaaatctt gtctccccgg gcgatggtgc cctcaaatga gtaagcatcc
     5641 acgcccagca cgctgccgta atacttcccg cctgggaacc gctcggggaa gaactcggtg
     5701 acgtgacgat ccatcggcgc tgggaaggca ttgaactcgt cgaacaactc ccaggcgaag
     5761 ggatacgcca gcttgccggt gtcaggggcg tgtccgaacg aactggccca actgtcgcgg
     5821 tcgaggccct tggcacgccg ctcggcgact ttcgtgcgaa tgaccggcca cagatcttcg
     5881 ccatcgagcc ggagatctgt gatccacgtc agatgattca atcccaccca acgggcagtc
     5941 acacgattgc ggggcgcgcc ggccatcgcc gccaggactt caatcccttc ttccgtcccc
     6001 atgcagaggc cgaatgcggg gagggaagtg gtacgccgta aagccctgac taccgccgtc
     6061 atcgggttcg agtagttgat gaagattgcg tacggacaca aacgttccac gtctttcgcg
     6121 atggcaacca tcggcgggat catccgctgc gcgcgtgaca ctccgcctga catcactgag
     6181 tcgcccaccg gctggtagac gccgaacttg cgcggcacga acacatcttg ctcccacgcc
     6241 cgccggctgc cgacgccgat cgtcgagatc acgaccgtcg ccccggcgag aacgtcgcgc
     6301 cgttccgtcg acgcggtgac ctttgcgggc tggtcgctcg tcatcatgta gcggcgcacc
     6361 aatttctcgg tagcctcgag gatcaccgga ttgatgtcga caagggcaat ctcccagtcg
     6421 cctttcggcc gccgctggag ccagtcgatc acgatgccct gcgtgaacat ggcgctgccg
     6481 gcgccgatga gcaccatctt ctgcttttcc ctagcggcga aggctccggg tttcacggag
     6541 gtggccgtcg aggcggcctg tgcggcagcg ggcgccgcgg acagcattcg aagaacatca
     6601 cgtcgattca tagtttctcc cgagtggaac ggcgaaacga ttccagcgtc caagtgtcgg
     6661 tttcggtatg tcgcagaacg taaagattat catcgtcggc gcgcacgcga aaccacgtat
     6721 caccgggtcc ataccaacga tctaataact ccaccacatc aaactgccgg tcttcgatgt
     6781 agaagcggag cgggcgctca tccgccttgt agccggcgta gcactcgacc cgcacctgga
     6841 aggcagggcg cattagagtt tatcgtacac gcccttccca ccggagtccc aggcgccaga
     6901 gacggggagc tccgaccgtg ggggtcgggg taaaaccggt gaggtattcg cggtcgaaga
     6961 gattctcgac cgaagcgaac gcactgagac gagcgttgat ctgcctccgg acggcgactt
     7021 gcacggcgcc gaaacccggc agcaggaagc gattcagctc gtcctcgaac tgaaggctgt
     7081 agctgcgcag gccggcgctg acgaacgtcg cgccgcgctg ccagccgagc cgcgcggaac
     7141 cctggtggct gggaacctgc ggcaggcgct cgccagtcga aaagcgcgaa tcggcataga
     7201 gataagaagc gtcgccgaac caatttctcc agcgatggtc cgcgtgcatc tcgacgccgc
     7261 gagcgagtgc ggaggcggcg ttccgcctct gtcgcacgat caggctcggc gatgaagtca
     7321 acgttacgtt cgtgatcagg tccgagagcg agttccgata cagggtgaaa ctcatatgcg
     7381 atcgttcgcc cgcgtagtca atacccgcct cagcaccgaa caggcgctca gaccggagcg
     7441 catcgttggc gcgcgtgacg gcattgccca cgcggaactc acgaaacagt tcgttcagcg
     7501 tgggagcgcg gaatgagcga tacgctgaag tccggacgcg caagcgcccg cgtcccgccg
     7561 agactccggc gctcggactg aaaaactgcc tgtccctgcc ggtaaagtca tggcgagctc
     7621 cgaggtggaa cttcaccggt ccggctcccg cgtccgtctg gacgaacgtg ccgtgctgga
     7681 gcagagtacc gccgccgacc cgcaggccgg ccggaaacaa cgtgtctttg ctgtagccct
     7741 cgacgtggtt gacgtcggcg ccggcgagga cgtcccaccg gcttccgttc cggctccagt
     7801 aaaccgcgcc gccgacagac tcggtcggta cgctttgcct gaacgtcggg cgctccgaat
     7861 tcctatcggc tgcgatggcg gaaaacgcgc tacgaaactc ctcgcgcgtg tgggatgcaa
     7921 gcacagagaa ggtatccgcc ccgcgtgcga gcgagtagtg cgcggacaac gaaccgaggc
     7981 tggtcgaatt ttgctggatt gaggtgccgt tgcgccggcg ctccgcgagc atgtcgaact
     8041 tcaggaacag ccggcttgcc gcaccgaaaa agtcgagccg ggaactagca gcgacaaagt
     8101 cggcgccggc tcggcgatcg gctgtccccc gaatcccctc agggaccaca tagtagccgt
     8161 cggtcgcaaa cgtgcggccg tgcaaagagg cggaccaacg ggagccgagg tgagtgaaac
     8221 cagcgccggc ttcatgggtc cccgcatttc cacctgagta gctggcggtg aggtgaaggg
     8281 gctcgggctg gcgcgagaac agcgcaatgg cgccgcccat ggccctgtca ccgaatacac
     8341 tggtggacgc gccgcgcgag atctctatac ggtcgatgtc gtcgggagcg aatcgagacc
     8401 actggaccca gcccccgaat ggatcgttca cggggaagcc gtcccaaagg acgagcgtcc
     8461 ggctggcacc ggtggacccg atgccgcgca gcgagacccc ctgcgtggtc ggatgcgcaa
     8521 ccacgctgga ggatcgccgg aaaagactga agccgggcac gtcgcggagg cgatcgtcca
     8581 ggttcactcc tggtgtcgcg cgaatatcgg agtcgtcaag gaccgtgatc gatgcgggcg
     8641 cgtcagcggt gatccgtccg accaccgtga tcgaactctt gaccggctct ggtggcgtcc
     8701 cggcgaggag gaggagcggg gagcagatca ggacccacaa cgcaggcgct gcgccagcgt
     8761 gcgacgggat accgctccgg cgacgcgctg aaaacgatct gaaagctatt ctcctcatga
     8821 ggacctgcag gcggtttgga cgccttgggc gagaccatgg ccagatactt caggatagta
     8881 tagatgtcgc ctgttgccgg taccgttagc cggaagtctt gtgtactgaa gcacacacgc
     8941 gcctcgcgcc ggaccacgtg ccatgtggtg tatgcacagc agtccaccgc ccggtcgaac
     9001 cactcgcggt gcgcatccgg gacgtcgagg tcgaggagga tctcaaccag cggctcctct
     9061 tctcttgcga actcccggac ctgcaattcg ttggtgtgcg cggtcgcctt ccagtcgacg
     9121 tggcgggcgc tctcgaaagc gacgtacggc cggatgcggt agaagtcatg acccctgccg
     9181 cggtagtgtg ctacgatctc gccgctcagc gtatcgagca gttcctcgaa cccgggctgg
     9241 ggatccaggc acggatacac cagaatgtct cgccgcaatg tgacgttagc gcgtctttcg
     9301 agaaatccaa atggaaacga cgtcgaaaac tcgaaaccat cctcgcgatg cagaccgcgc
     9361 cggtcaaaac gcacctcaac ggtctcctcg agcgtcgaac cgccgggtaa gacaggaaaa
     9421 tacagcggcg tagaaaacgc gctctccccg acaccggcca cttcgaccga gaacgacggc
     9481 atccagcgct tcccgttccg gacgaaaatc cgcgctggaa acgtccggcg cgcggagaga
     9541 tgttccggca ggtgaaagtc gagttccaag ccggccagag acaggcggca gatgaaattg
     9601 gaaatcatga ggatcgaaat catcgccgcc agaatcagga acaacaagtt gttggccgag
     9661 acgaccgcgg tgaaggcgac gagcagcgcc gcgacagcaa agaccaagcc tatgggagtg
     9721 gtccttcgct gcgcgctata gcggaggaat ttctgaaagc cgcttcggag ggcgctcaac
     9781 ggcacgaccg catcatggca caattgagga attcatgtcg accgaagcgc caacagcggc
     9841 cccggacgga gggccgaggc taaaacgggc actgagtctc tgggacctcg ttttcaccgg
     9901 gatcatcctc atccagccaa cggccccgat gccgctgttc ggcatcgttt accaggaagc
     9961 ccgcgggcac gtggtaacca ccatcctcat cgccatggtc gccatgctct tcaccgccat
    10021 cagttacggc cgcatggcgc gcgcctatcc gagcgccggc tccgccttcg tctatgtcgg
    10081 gcgggagatc catccctcca tcggctatgc gagtggctgg agcatggcca tggattacat
    10141 gctcaacccc gtcctatgca ccgtctggtg ctcgaaggcg gcgggcaaca tcctgcctga
    10201 ggtcccctac tatgcgtggg tagtgttttt cgcgctgcta ttcactgtat tgaacctgcg
    10261 gggtatcaaa tcctcggcgc ggaccaacga gtggctcgct gccgggatgg gcgtagtgat
    10321 cctgattgtc ctggctgcgg cggtacgcta cgttcttcga ctgtgtcccc aggagacgga
    10381 gtttttcacg cggccgtttt acgatcccga aaccttctca atcaaggcag tgttcacggg
    10441 aacttcgatt gcagccttga cctatatcgg ctttgatggg atttcgaccc tctccgaaga
    10501 ggtccagaat ccgcggcgaa acattctgct ggcgacggtg ctcacctgcc tgatcactgg
    10561 ctttctggcg agcattgaag tctatgccgg ccagttgatc tggccggctg gccaggcatt
    10621 ccccgacgcc gacacggcct acgttcacgt ggcgggccgc gccggcggac tgtggctgtt
    10681 ccagttggtc aatctgactc ttctggtagc gacgatcggt tcggggaccg gctcgcaact
    10741 ggcggctgct cgcctgcttt acggaatggg ccgcgacaat gccatcccaa agcgcttctt
    10801 cggtgcgatt gagccgaagc ggggaattcc acgcaacaac gtgctgttca ccggagggct
    10861 cgccctgtgt ggcgccttcg ttctgagcta ccaactcacc gcggagctgt tgaacttcgg
    10921 cgcattcatc gccttcatgg gggtgaacct ggctgccttt acccggtact accttcgtgc
    10981 gaacgaaaag aagctgatca acgccgtgcc gccgctggtc ggcttcacta tctgcctgta
    11041 catctggctg aacctgcgca ctcccgcgat gatcgccggc gccgtgtggc tcgcgatcgg
    11101 tattgtttat gg
//
LOCUS       DATIFQ010000246        24122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_79358, whole genome shotgun sequence.
ACCESSION   DATIFQ010000246 DATIFQ010000000
VERSION     DATIFQ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24122
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79358"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VLE22_11315"
     CDS             complement(<1..750)
                     /locus_tag="VLE22_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15040.1"
                     /translation="MFPINRRKWMTLSGAAFALRGASAGVRQAPAPGAAQATPDELLL
                     KDYRPKPIFRIPKTDIKKAKYPVIDVHSHGPRPIEQLPEFVKTMDAANVEKTVILTGA
                     NSVERFAEISRVYSSYPGRFELWCGFDLTGFGEQGFGPNAVKALEECHRLGAVGVGEV
                     SDKGWGFRTSAARRSAVGATPAGQKPAPGPGPHPDDPRIDALWDKCAQLGMPVNIHVS
                     DPIWAYEPMDKTNDGLPNGYKWRIDDKQPGIF"
     gene            905..1612
                     /locus_tag="VLE22_11320"
     CDS             905..1612
                     /locus_tag="VLE22_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB15041.1"
                     /translation="MARFARLVVPGFPHHITQRGNHRQDVFFSDNDRRMYLAILRDHV
                     HRQNVRILAWCLMTNHVHIVAVPSRDDSFALAFGRTHNEYARWLHVRQRRVGHLWQNR
                     FHSCPLEGRHLWEAVRYTELNPVRAGVVCAARDWRWSSAQAHLGGGDEWNLVDLIPWR
                     ERWTSETWRQALEAASGDSAFAVRLREATQTGRPCGESEFTKMLEAETGRSLKPQKRG
                     PKPKTTVESGQMSFGIA"
     gene            complement(1748..3925)
                     /gene="lptD"
                     /locus_tag="VLE22_11325"
     CDS             complement(1748..3925)
                     /gene="lptD"
                     /locus_tag="VLE22_11325"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HSB15042.1"
                     /translation="MLVRAVTQTAEGHVYRLRGSAQLETTEVQLWADEIDYDEDAGQA
                     EARGNVHFKQLERGEELWADTVEYDLKEETGKFYNVRGTSPPKIDPRPGLLTTSSPFY
                     FQGDWAERLKDKYVLHHGMITNCRLPKPWWTLRGPKFDIVPNDRALAHNSVFRLRGVP
                     LFYFPMFYKSLEPRPRKSGLLTPNIGNSSRRGKMVGGGYYWVINRSFDAAYRAQYFTQ
                     RGVAHHVDFRGKPTRSSDFNAILYGVNDRGPILPNGKRGAKEGGFILSFEGRSDFGQG
                     FQGQAEVNYLTSLRFRQSFTESFNEAILSEVHSIGFVGKHWSSYGLNLVLARGENIQR
                     LGDYDAATGKFKPDDKITIRKLPQIEFNSRDRQVRDGALPVWVSLESTAGLVRRNQPL
                     FQTRHFQERMDFRPRVMTAFRWKGFSLLPSFSVRETHYGERFENGKVLDENINRFSRE
                     VTLELIPPSLARTFHGPGWLGEQVKHVIEPRVVFRHAGGIADFDKLIRFDETELITNT
                     TEAEFSLINRLYAKKGGQVNEVLSLQVWQRRYFDPDFGGAVVEGRRNVVQSAAELTPF
                     AFLDRPRHYSPVVSVLRLSPVPRFGLEWRSDYDPLRSKVTNSGLTADWRFSSYFVSMG
                     HNQVRSVPLLTPSANQFRGLLGIGNDNRRGWNAAFSAIYDYRLGVMQFATTQVTYNTD
                     CCGFSVQYRRFSFGTRNENQFRIAFSVANIGSFGTLKRQERIF"
     gene            4153..5019
                     /locus_tag="VLE22_11330"
     CDS             4153..5019
                     /locus_tag="VLE22_11330"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSB15043.1"
                     /translation="MNCRYCGASNTEDEHRCRRCGRRIVPGVPEPQRFAAVPAPRPAP
                     PGPLQSPPPPVRRAVPHQQALFADAAPAGPRVIPFDSHAPGARQAPRPGNPPLPAAAR
                     APRTEAPKRPPARPPSQAQRSLEFLPPAPPAPSARRTSVESSIYCDARVAPLGQRISA
                     AVLDVFVVLAGFVLMLSVFAYHTGGLVAGRAAYIIYGLAFALLLLFYKYASFRWAGIT
                     PGTRWAGLRLLDFDGRPPARRDRLNRLAAGCLSLLPAGAGLLWAIVDEERLTWHDHMS
                     KTFVTPAVRRPS"
     gene            5061..6455
                     /locus_tag="VLE22_11335"
     CDS             5061..6455
                     /locus_tag="VLE22_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15044.1"
                     /translation="MRIHFDDTNLRAEMVGEAHGLTTAEIREFTPRALDALRGFRKLS
                     EDGRYGFPHLPFQTAVAKEITAYAAEVRKSYDTVCIVGIGGSALGAWALDCGLRGPHP
                     VQGEFSSTFPRLVILDNVDPSFVGAAIESMNPKRTLVVVIAKSGSTAETVSTFLIVRD
                     WMRRKLGDKAGRRIAAVTSRERGELAVIAARENYRVFHIPDNVGGRFSVLSPVGLLPA
                     ALIGADIRKLMKGAAEMTHSCWRENLDANIALRAALFHYLAWVKKGKTIQVAFPYSNR
                     LWGTAFWFRQLWAESLGKARTRSGEEIHSGQTPVAALGATDQHSQVQLYMEGPNDKLF
                     SFWAVKNFGAAGHIPRSRTGLESFDYLAGRSLMELITAERVSTAAALTENGRPNCTFT
                     LDRVDEAHLGAFLQLLEFETAFAGELLNIDAFTQDGVELGKRFSFGLMGRAGYETYRE
                     RYENYQKKRQKVTV"
     gene            complement(6463..6651)
                     /locus_tag="VLE22_11340"
     CDS             complement(6463..6651)
                     /locus_tag="VLE22_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15045.1"
                     /translation="MVVKFLLFCILLVLCWPLAILALFLFPLVWLLLLPFRLVGLVVT
                     GLFEFIRALFLLPARILR"
     gene            complement(6727..7503)
                     /locus_tag="VLE22_11345"
     CDS             complement(6727..7503)
                     /locus_tag="VLE22_11345"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HSB15046.1"
                     /translation="MSLKDRTALVTGGSRGIGSAICVRLARNGAAVAVNYLERRREAE
                     ELAEEINSEGGRAFAIQADVSDPAAVREMVRTVASELGPTDILVNNAGLFRRGDLDDF
                     SSSALEAMRRTNVDGVVNVTLAVAEGMKARRWGRIVNLTSIAAHGTTMAGTTFYAATK
                     AAVAVLTRRFALELGPDGITVNAIAPGFIVTDMVYADRPPDEVEQLLAGISNKAMVRR
                     TGRPEDIAHAVAFLVSEEASFITAQILTVDGGRTDYICHP"
     gene            7781..10516
                     /locus_tag="VLE22_11350"
     CDS             7781..10516
                     /locus_tag="VLE22_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HSB15047.1"
                     /translation="MKKLKSETETTILQRRQQPLDVFFSPENVAVIGATETPGSVGRT
                     VLWNLISTPFGGTVYPVNLKRSSVLGIKAYPNIGAVPGEVDLAVIITPAAAIPGVIRD
                     CAAAGVQGAIVISAGFKELGPQGAELERQVLVEAQKGGVRIIGPNCLGVMSPPTGLNA
                     TFARGMARPGSVGFISQSGALCTAVLDWSFREMVGFSAFLSIGAMVDVGWGDLIYYLG
                     EDPKTKSILIYMESIGDARSFLSAAREVALTKPIIVIKAGRTAAGGKAAASHTGSMTG
                     SDDVLEAAFRRSGVLRVNQISDLFYMAEVLAKQPPPKGPKLTILTNAGGPAVLATDAL
                     ITSGGELTELSPETFEEFNRILPPTWSRNNPVDIIGDATPERYAKALATAAKDPNADG
                     MLVVLTPQDMTDPTQIAEQLRPYANMGGKPVLASWMGGPEVAAGERILNAAGIPTFSY
                     PDTAARMFNYMWQYSENLRALYETPVFPAGAPEVEVSRGKAETIIAGARAEGRTILTE
                     FESKQVLAAYGIPTVETRVARSEQEAVAAADAIGYPVVLKLFSETITHKTDVGGVQLN
                     LKDAGAVREAFRRIQTSVAEKAGAEHFLGVTVQPMIKLDGYEIILGSSLDPQFGPVIL
                     FGLGGQLVEVFKDRALALPPLNTTLARRMMERTKIYTALKGVRGRKPVDLAALEQLMV
                     AFSRLVVEQRWVKEIDINPMLASPERLIALDARVVVHEPTVQESDLPRLPIRPYPTRY
                     VSPWTTRDGSDVLIRPIRPEDEPLIVKFHETLSERTVYLRYLHMMKLSQRVAHERLVG
                     ICFIDYDREIALVAEQKDPKTGEPRILAVGRLRKLHGTNDGEFAVLVSDEYQGRGLGI
                     ELVRRLIEVARAEKLSAIVGDVLQDNAVMRHICETLGFTMEGSIEDPVLKAEYKL"
     gene            10583..11041
                     /locus_tag="VLE22_11355"
     CDS             10583..11041
                     /locus_tag="VLE22_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HSB15048.1"
                     /translation="MLVIGCGNGDRGDDAAGILVARRLRAQGIEAREQSGEALALMES
                     WRDAPGEQEVILVDAVVTGAPPGTVTVWDARTAPVIGDFFRCSTHAFGVAEAIELARI
                     LDLLPPNLLIYGIEAGSFDIGSEPSPEVLAAVEQVTDRIGSEIGVAPGRN"
     gene            11076..11420
                     /gene="hypA"
                     /locus_tag="VLE22_11360"
     CDS             11076..11420
                     /gene="hypA"
                     /locus_tag="VLE22_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HSB15049.1"
                     /translation="MHELALAQSILEICEEEARKRQAGAIHKIKLRLGDFSGVVREAL
                     EFSFDALKVETLAAGAGLEIERVPLEVRCPRCGQVSSPPASDLCFVCTECGSPVEITS
                     GRELQVEYIEVE"
     gene            11451..12119
                     /gene="hypB"
                     /locus_tag="VLE22_11365"
     CDS             11451..12119
                     /gene="hypB"
                     /locus_tag="VLE22_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HSB15050.1"
                     /translation="MMERVPVVRNVLQANEEAAAANRELFDRGGMLAINLMSAPGAGK
                     TSVLEQTIRRLGTRYRLAVIEGDLQTSIDAERIRAAGVPSHQITTGTVCHLDARMVSK
                     AVAEFPMDGVRVLFIENVGNLVCPAEFYLGEHLRVMVYSVVEGAEKPKKYPLMFHTSD
                     VVLLNKVDLVPYAGVEIEDLKKNVLEVNPRATIFPVSCRTGEGLDAWTEWLDSAVRES
                     AATA"
     gene            complement(12116..12520)
                     /locus_tag="VLE22_11370"
     CDS             complement(12116..12520)
                     /locus_tag="VLE22_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSB15051.1"
                     /translation="MDKLDQLKLKYQSVINLAIQKGVRLSHVHVEKDKLLIQGAAPSE
                     AVKNDVWNQIKLVDASYSDLTCDLKIDASLAPAPKTYTVVAGDSLWKIAEKQWGKGKG
                     NLYPEIIKANPGKLKDDKTVIHPGDVLVIPEL"
     gene            12575..13099
                     /locus_tag="VLE22_11375"
     CDS             12575..13099
                     /locus_tag="VLE22_11375"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HSB15052.1"
                     /translation="MQELLNTILQAQGGGLVKQLAGQFGIDESQAGSALGQLLPALTG
                     AAQNNVRQGGLDGLLGALSSGGHERYIEDPSLLGSQETVEDGNGILGHLLGSKDASRA
                     LASQVSGSTGLGVDVLKKMLPVVATLVMGSMSKATNQGSTGSGLLSMLDTNRDGSVLD
                     DVAGFMGKFLTKRI"
     gene            complement(13218..13559)
                     /locus_tag="VLE22_11380"
     CDS             complement(13218..13559)
                     /locus_tag="VLE22_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15053.1"
                     /translation="MLSVGLFVRLEAKPGKEEEVAAFLRQGLQLANEETTTPLWFALR
                     LGPSTFAIVDAFPDESGRQAHLNGPIAKALMAHAPHLLAVPPSIEKTEILGAKLSLEV
                     RNAMAASRAAL"
     gene            13662..14621
                     /locus_tag="VLE22_11385"
     CDS             13662..14621
                     /locus_tag="VLE22_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB15054.1"
                     /translation="MRVNVLVLDGVFDLGLSAVLDAFQTANELIGISGLTVPRFEVKI
                     AGVRKAVKTSQGLTVPVNATGWRTPNCVVVPAIGFKMPGPLESALARPDVQDAGASLR
                     QWARLGATMTAACIGTFVMAESGLLDHHHATTTWWLAPLFRKRYPQVLLDESNMIVKS
                     GKFVTAGAALSHMDLALWLVRGVSPTLASLTAKYLIVDSRPSQSAYALTDHLVHSDPL
                     IQRFESWARSRLKHGFSLDDAAEAVGSSKRTLARRMQTVLGKSPLSYFQSLRVERAVH
                     LLKTSNASVDEIAERVGYTEGSILRVLLRRRLGLGIKEIRRSA"
     gene            15119..16024
                     /locus_tag="VLE22_11390"
     CDS             15119..16024
                     /locus_tag="VLE22_11390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HSB15055.1"
                     /translation="MTISRRQFLELFRNSAAALGVGAVNLSVLEKALAQPGGPTVIWL
                     QGSACTGCSVSLLNRISSTSPSTTADVLINIINLRYHPNLMAAAGKSAAAIAEEIAGG
                     SGHILAVEGGVPTAFGGAACWAWTRADGTDVTFQQAVTMLAANAAQILCIGTCAAWGG
                     IPAALPNPTGVRSVREVTGRPTLNIAGCPTHPDWIVWAIGQLVSGAPISVDSYGRPAA
                     LFGKTVHELCPRREREEASTYGQDYLCLEELGCKGPSTKGNCPITLWNGRANWCCDSN
                     TLCIGCTDPAFPFTNLRSKTYAIDD"
     gene            16008..17519
                     /locus_tag="VLE22_11395"
     CDS             16008..17519
                     /locus_tag="VLE22_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HSB15056.1"
                     /translation="MPSTIKIDPVTRLEGHLKIEVTVDGVAGAQKVVDAKATGTLFRG
                     FEAILKNRNPLDAPDITQRICGVCPVSHGLAASMTLENAAGVTAPDNARIMRNLVLGS
                     NFVQSHILHFYHLAVQDFVSGPDMPPWQPNWAADKRFDAATSGVLVSHYVQALEMRRK
                     AHEMGALFGGRLPHPPAFVPGGFTTTPRPERITKFRAYLAELIAFINNVYLPDVGALA
                     NAYADYYSIGRGHGNLLAYGVFDLDGAGQSKLLRRGRILGGTGSALAVDPKAITESVT
                     YSWYSDTTNNLSPSSGVTQPVYPKGGAYSWLKAPRYGGASRRKGKRVTVTPGAPYETG
                     PLARMWVNGEYTRGISVMDRHASRAQETLKVAQAMDVWATQLGSGPVYQQYVTPTSAS
                     GFGLTEAPRGALGHWVDISNGKIGNYQVLTPTCWNASPRDSAGVPGPIEQALVGTPVA
                     NLDEPVEVLRVVHSFDPCLSCAVHVMRPAEGSKIFALGHYHGEDEVHEHTHSH"
     gene            17497..18018
                     /locus_tag="VLE22_11400"
     CDS             17497..18018
                     /locus_tag="VLE22_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HSB15057.1"
                     /translation="MSTRTRTDGNGPSAPPRRGRRILVAGLGNLLLMDDGVGVHAVRE
                     LRRHAGSGVMVAEVGTAVLDALHLIEKADRLLAIDAMRAGGAPGTIYSFGVEDVKQRP
                     TEASLHELNLLSALTLTRRRPREITILGVEPGRIDYGLELTPAVQEALPRLVSAASRI
                     VAKWVGGTFGRDG"
     gene            18081..20357
                     /gene="hypF"
                     /locus_tag="VLE22_11405"
     CDS             18081..20357
                     /gene="hypF"
                     /locus_tag="VLE22_11405"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HSB15058.1"
                     /translation="MTVSSAERLRVTLHGAVQGVGFRPFVYRLATELGLPGWVLNSSA
                     GLSLEVEGPRDRLDTFLARLESEKPPAAVILTKEASFLAPVGFTGFEIRTSDASEGKT
                     ASVLPDLATCGDCLAEMRDPADRRCGYPFTNCTNCGPRYTIILDIPYDRTNTTMFGFG
                     LCAECGREYRDPGDRRFHAQPVACPACGPKVWVELPQGMEMQGRMGTDSHFATAAKWN
                     LSPFSPIFVAAALERGEIVALKGIGGFQLLADARNEQAVERLRQRKHREEKPFALMLP
                     SIEEVRRYCEVSPAEERLLKSAAAPIVLLRPNGTPGIAPSVAKSSPYLGVMLPYSPLH
                     HLLMREFPAPVVATSGNRSDEPIAIDNDEARDRLGEIADVFLMHDRPVARPCDDSVAR
                     VTRGRESVVRRARGYAPLPVRVARELPPVLAVGGHLKNTVAIAIGRDVYLSQHVGDLD
                     TAEARGAFERAIEDLCRLYRFEPQMAACDLHPDYASTQWARASGLPVIAVQHHHAHAA
                     ACAAENDIRGDYLGVSWDGTGYGLDATVWGGEFFLVRDAEFERVAHLRPFRLPGGEAA
                     IREGWRPAASLLWEVFGPEGVPESAQKPVLLRMLERGVNAPLTTSVGRLFDAVASIAG
                     IAQANRFEGQAAMQLERAIGATVTAECYRLGEGGDWAPLVEEVGADAARNVSPALISA
                     KFHNALARWIVEVAEQTGVGQVALSGGVFQNTYLTERAASLLEERGFRVYTHQRAPAN
                     DGGIALGQAVIAGIRAGG"
     gene            20362..20634
                     /locus_tag="VLE22_11410"
     CDS             20362..20634
                     /locus_tag="VLE22_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HSB15059.1"
                     /translation="MCLAVPGKIVSVMEDDPPEVRRGKVDFAGVQKEVCLAYTPEAKI
                     GDYVLVHVGFALNVVDAEEAQRIFETLDEMQRLEELAEAERAESGP"
     gene            20631..21761
                     /gene="hypD"
                     /locus_tag="VLE22_11415"
     CDS             20631..21761
                     /gene="hypD"
                     /locus_tag="VLE22_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HSB15060.1"
                     /translation="MKYIDEYRDPERVRQLAAAIRRKITRPWTIMEVCGGQTHTIVKS
                     GLQELLPEEVTLVHGPGCPVCVTPLELIDSAVAIASRPDVTFCSFGDMLRVPGSTKSL
                     FDVKAEGGDVRIVYSPLDALKLARANPQRQVVFFAVGFETTAPANAMAVWQAEREGIA
                     NFSILVAHVLVPPAIEAILSSPNCRVQAFLAAGHVCTVMGFEEYEPVAKRYRTPIVVT
                     GFEPLDLLEGVHMAVAQLEEGRAEVENQYSRAVRREGNRPARDRVAEVFEVGPRQWRG
                     IGEIPASGLRLRERFRKLDALERFATGVGHVKEPAICIAGLVLQGLKKPYECAAFGKQ
                     CTPETPLGAPMVSSEGACAAYYLYGRHRHDAVRAGVSSAEVH"
     gene            21718..22764
                     /gene="hypE"
                     /locus_tag="VLE22_11420"
     CDS             21718..22764
                     /gene="hypE"
                     /locus_tag="VLE22_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HSB15061.1"
                     /translation="MTPFELACPAPKFTEDRVLLAHGGGGRLMQRLIEQVFLPEFRNP
                     ALEALHDGAVVDVNGSRLAFTTDSYVVRPLFFPGGNIGELAVYGTANDLAMCGARPLY
                     LSAAFILEEGLPMETLRAVAASMREAAARAGVTLVTGDTKVVDKGKGDQMFITTAGVG
                     TLSEAVRIGPAFVEPGDAVIVSGDLGRHGVAVLSVREGLEFEAPVESDAAPLWEPVEA
                     LLRSGIEVHCLRDLTRGGLASALNEIAGSGGVGIGIDEALVPVREAVKGACEILGLDP
                     LYVANEGRFAAFVPKAQAELAVETLRMVGVSEGAAVIGEVSAEHPRTVVLRSRIGGHR
                     VLDMLSGEQLPRIC"
     gene            complement(22815..23156)
                     /locus_tag="VLE22_11425"
     CDS             complement(22815..23156)
                     /locus_tag="VLE22_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HSB15062.1"
                     /translation="MSRPAVSPDTETTRREQLMPLFHVVLLDDDDHTYDYVIEMLQRL
                     FLLSQFEAFRHAVEVDTTGRTIVITCELPQAEFARDQIHGYGPDPRMPKSKGSMSAVL
                     EPASGIPQATD"
     gene            23251..23601
                     /gene="ssrA"
                     /locus_tag="VLE22_11430"
     tmRNA           23251..23601
                     /gene="ssrA"
                     /locus_tag="VLE22_11430"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(23626..23847)
                     /locus_tag="VLE22_11435"
     CDS             complement(23626..23847)
                     /locus_tag="VLE22_11435"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSB15063.1"
                     /translation="MKRYAVVFEKAGNNWGAYIPDLPGCVTTGKTLEETKRNIREAIE
                     LHLEAIREVGEPIPEPTSDVDFIELQTVA"
BASE COUNT         4452 a         7306 c         7974 g         4390 t
ORIGIN      
        1 gaaaatcccc ggctgcttgt cgtcgatgcg ccatttgtag ccgttcggaa gcccgtcgtt
       61 ggtcttgtcc atcggctcgt acgcccagat ggggtccgaa acgtgaatgt tgacgggcat
      121 cccgagttgc gcgcacttat cccatagagc gtcgatgcgc ggatcgtccg ggtgcggccc
      181 ggggcccggc gccggcttct gcccggcggg cgtggctcca accgcgctac gacgcgccgc
      241 cgaagtccgg aagccccatc ctttgtcact gacttccccg accccgacgg cgcccagacg
      301 atggcactct tccagcgctt tgactgcgtt cggcccgaat ccctgctcac cgaagcccgt
      361 caaatcaaaa ccgcaccaca actcaaaacg cccgggatag ctggaataga cccggctgat
      421 ttcggcgaac cgctcgaccg agtttgcgcc ggtcagaatc acggtctttt ccacgttggc
      481 ggcgtccatc gttttgacaa actcgggaag ttgttcgatg ggccgcggtc cgtgcgaatg
      541 aacatcgatg acgggatact tcgccttctt gatgtccgtc ttcgggatcc tgaaaatggg
      601 cttcgggcgg taatccttga gcaagagttc gtccggcgta gcctgtgccg cgcccggcgc
      661 cggagcttgc cgcacgccgg ccgacgcccc gcgcagcgcg aacgccgcac cggaaagcgt
      721 catccatttt ctgcgattga tcgggaacat ttctcgcgcc tcctgtcgct gctagtcacg
      781 gaatcgggaa cattatagtc aaaggtgatg gtcctctggc cagcggcttc ctgaaacggg
      841 gacaggctac gaaattccga aacccggggg agaaaagcgg cgtggcggca gcactgtttc
      901 tcctatggct cgcttcgccc gtcttgtcgt accgggcttc ccccaccaca tcacccagcg
      961 cggcaaccac cgacaggacg tgttcttcag cgacaacgac cggcgcatgt acctggccat
     1021 cctgcgagat cacgtgcacc gtcagaacgt gcgtatcctc gcatggtgtc tgatgacgaa
     1081 ccatgtgcac atcgtcgccg taccctcgcg cgatgactcc ttcgcgctgg cgttcggcag
     1141 aacgcataac gagtacgcgc gctggctcca tgtgcggcag cggcgcgtcg ggcacctgtg
     1201 gcagaaccga tttcattcct gcccgctcga gggccggcac ctgtgggagg cggtccggta
     1261 tacggaactc aacccggtgc gggccggggt ggtttgcgcg gcccgagact ggcgctggtc
     1321 gagtgcgcaa gcgcaccttg gagggggcga tgaatggaac cttgtggatc tgataccgtg
     1381 gcgggagcga tggacaagcg aaacgtggcg acaggcgctg gaagctgcat ctggcgactc
     1441 tgccttcgca gtgcggttgc gcgaggcgac ccagaccggg cgtccgtgtg gcgagagcga
     1501 attcacaaaa atgctggagg cggagacggg aaggtcgttg aagccgcaga aacgtggtcc
     1561 caagccgaaa acaacggtag aatccggcca gatgagtttc ggaattgcgt agcctgtccc
     1621 cgtatctcgt atctcccgta tctctgtcca caagtcagtg ttgtttatcc tcgaatcaag
     1681 tcgcgaagcc ggctacgcaa tcgctaagtt tgcgtaaaca ccttggtcct cacgatgttc
     1741 ctcggcatca gaaaatccgc tcctggcgct tgagggtgcc gaaggagccg atgttggcca
     1801 ccgagaaggc aatgcggaac tggttctcgt tgcgcgtgcc aaagctgaag cgccggtact
     1861 gaacgctgaa gccgcagcag tcggtgttgt aggtcacctg tgtggtggcg aactgcatca
     1921 ccccgagccg gtaatcgtaa atcgcgctga acgccgcatt ccagccgcgc cggttgtcgt
     1981 tgccgatccc cagcagccca cggaactggt tggccgaggg cgtcagcagc ggaacgctcc
     2041 gcacctggtt gtgccccatc gagacaaagt agctcgaaaa ccgccagtcc gccgtcaggc
     2101 cgctgttggt caccttgctg cgcagcgggt cgtagtcgct ccgccactcg agaccgaacc
     2161 gcgggaccgg gctgagccgc agcaccgaca ccaccggcga gtaatgccgc ggccggtcca
     2221 ggaacgcgaa cggcgtcagc tcggccgcgc tctgcaccac gttgcgccgc ccttccacca
     2281 ccgcgccgcc gaagtccgga tcgaaatacc gccgctgcca cacctgcagg ctcagcacct
     2341 cgttcacctg cccgcccttc ttcgcgtaga gccggttgat cagcgagaac tcggcctcgg
     2401 tggtgttcgt aatcagctcg gtctcgtcga accggatcag cttgtcgaaa tcggcaatgc
     2461 cgcccgcgtg ccggaacacc acccgcggct cgatcacgtg cttcacctgc tcgccgagcc
     2521 accccggacc gtggaacgtc ctcgccagag aaggaggaat cagctccagc gtcacctcac
     2581 gtgaaaaccg gttgatgttc tcgtcgagca cctttccgtt ctcgaaccgc tcgccgtaat
     2641 gcgtttcgcg cacggagaag ctcggcagca ggctgaaacc cttccatcgg aacgcggtca
     2701 tcacgcgcgg ccggaagtcc atccgctcct ggaaatgccg cgtctggaag agcggctggt
     2761 tgcgccgcac caggcccgcc gtcgattcga gcgataccca gaccggcagc gcgccgtccc
     2821 gcacttgccg gtcgcgcgag ttgaactcga tctgcggcag tttgcggatc gtgatcttgt
     2881 cgtcgggttt gaacttgccg gtcgcagcgt catagtcgcc caggcgctgg atgttttcgc
     2941 cgcgcgccag cacaaggttc aatccgtagc tggaccagtg cttgccgacg aacccaatgg
     3001 aatgcacctc cgacagaatc gcctcgttaa aggattccgt gaacgactgc cggaaccgga
     3061 gcgacgtcag gtagttcacc tccgcctgcc cctggaagcc ttggccgaag tccgaacggc
     3121 cctcaaacga caggatgaac ccgccctctt tggcgccgcg cttgccattc ggcaggatcg
     3181 gcccgcggtc gttcaccccg taaaggatcg cgttgaagtc cgagctgcgc gtcggcttgc
     3241 cgcggaagtc cacgtggtgg gcgacgccgc gctgcgtgaa gtactgcgcg cgataagcgg
     3301 cgtcgaagct gcggttgatc acccagtaat agccgcctcc caccatcttg ccgcgccgcg
     3361 agctgttgcc gatgttcggc gtcagcaagc cgctcttgcg gggacgcggt tcgagcgact
     3421 tgtagaacat cggaaagtaa aacagcggca cgcctcggag acggaaaacg gagttgtggg
     3481 ccagcgcgcg gtcgttcgga acgatgtcga acttcggccc gcggagcgtc caccacggct
     3541 tcggcaggcg gcagttcgtg atcattccgt gatggagaac gtatttgtcc ttcagccgct
     3601 ccgcccagtc tccctggaaa tagaacggac tcgaggtggt cagaaggccg gggcgcgggt
     3661 cgatcttcgg cggcgacgtt ccgcgcacgt tgtagaattt gccggtctcc tccttgaggt
     3721 cgtattcgac cgtgtccgcc cagagttcct ccccgcgctc gagttgcttg aagtggacgt
     3781 tcccccgggc ctcggcctgg ccggcgtctt cgtcatagtc gatctcgtcg gcccagagct
     3841 gcacttccgt ggtctccagt tgggcggagc cgcggagccg gtagacgtgc ccttcggctg
     3901 tctgcgtgac ggcccggacc agcaattcgt ttcgtgcgag cggcggagtc tgggcggtgc
     3961 accacagggc cgaaaagaaa aaaaacaggc caagatgcgg aaaaacgcgc gaaatttcgt
     4021 gacaaagtcc gccaattatg ttacttacat gggaagggca cacgcggcgc gatgtgaaga
     4081 gaggaatcaa tcgaaaaccg tagcacgcag aaagaagtcc ccgcaagccg ccgcccggac
     4141 ccggatctcc ggatgaactg ccggtactgt ggagcgtcga acacggagga cgagcaccgc
     4201 tgccgccgct gcggccgccg catcgtgccc ggagttcccg agccgcaacg cttcgccgcc
     4261 gtaccggccc ccagacccgc gccgcccggc ccgctgcaat ctcctccacc tccggttcgg
     4321 cgcgccgtcc cccatcagca ggcgctgttc gccgacgcag cacccgccgg accacgcgtg
     4381 atcccgttcg attcgcatgc ccccggagcg aggcaggcgc cacgtcctgg gaacccgccg
     4441 ctgccggccg ccgcccgcgc gccgcggaca gaagcgccga agcgtccgcc ggcgcgtccg
     4501 cccagccagg cgcagcgaag cctggagttc ctcccgcccg cgccgccggc gccgagcgcc
     4561 cgccggacgt ccgtcgaatc gtcaatttat tgcgacgccc gcgtggcgcc tctggggcag
     4621 agaatctcgg ccgcggtcct cgacgtgttc gtggtcctcg ctggcttcgt cctcatgctg
     4681 tcggtcttcg cctaccatac cggcgggctg gttgccggcc gcgcggcgta cattatctac
     4741 ggcctggcct tcgccctgct tctgcttttc tacaagtatg cgtccttccg ctgggctgga
     4801 atcacccctg gaacccggtg ggcgggtctc cggttgctcg acttcgacgg gcggccgccg
     4861 gccaggcgcg accggctgaa tcgcctcgcg gccggctgcc tgagcctgct accggccggc
     4921 gccggacttc tctgggcgat cgtcgacgaa gagcgcctca cctggcacga ccacatgtcg
     4981 aagacgttcg tcacgcccgc tgtgcgccgc ccctcctgaa gcgttatcct gtccgcgccg
     5041 atctgtcgca agatattggt atgcgcattc acttcgacga tacgaacctc cgcgccgaaa
     5101 tggtgggcga agcccacggg ctcaccacgg cggagatccg cgagttcacg ccccgcgcgc
     5161 tcgacgccct ccgtgggttc cggaaattga gcgaagacgg acggtacgga ttcccccacc
     5221 ttccttttca gaccgccgtc gcgaaggaga tcaccgccta cgcggccgag gtgcggaagt
     5281 cgtacgacac cgtttgcatc gtcggcatcg gcggcagcgc cctcggcgcc tgggcgctcg
     5341 attgcggcct gcgcggaccg caccccgtcc agggagagtt ctcctccacc tttccgcgcc
     5401 tcgtcattct cgacaacgtc gacccttcgt tcgtgggcgc cgcgatcgag tcgatgaacc
     5461 cgaaaaggac cttggtggtc gtcatcgcca agtccggctc caccgccgag accgtgtcca
     5521 cgttcctcat cgtccgcgac tggatgcgcc gcaagctggg cgacaaagcg ggacgccgca
     5581 tcgccgcggt gacgtcacgg gaacggggcg aactggcagt gatcgccgcc cgcgagaact
     5641 atcgtgtgtt ccacatcccg gacaacgtcg gcgggcgctt cagcgtgctt tcgcccgtgg
     5701 gcctgctgcc ggcggcgctc atcggcgccg acatccgcaa gctcatgaag ggcgcggccg
     5761 agatgacgca ctcgtgctgg cgggagaacc tggatgcgaa catcgccttg cgcgcagcgc
     5821 tgtttcacta cctggcctgg gtgaagaaag gcaagaccat tcaagtggct ttcccgtact
     5881 cgaaccgcct ctggggaacg gccttctggt tccgccagct ctgggcggag tcgctcggca
     5941 aggcccggac gcgcagcggc gaggagatcc acagcgggca gactccggtg gccgcgctcg
     6001 gcgccaccga ccagcattcg caagtgcagc tttacatgga aggtcccaac gacaagctgt
     6061 tcagcttttg ggcggtgaag aacttcggcg cggccggcca cattccgcga agccgcacgg
     6121 gcctcgaatc gttcgactat cttgcgggcc ggtcgcttat ggagctcatt acggcggaac
     6181 gggtttcgac cgccgccgcg ctcaccgaaa acggccggcc gaactgcacg ttcacgctcg
     6241 accgcgtcga cgaagcgcac cttggggcgt tcctgcagct tctggaattc gagacggcct
     6301 ttgcgggaga actgctgaac atcgacgcct tcacccagga cggcgtggaa ctcggcaagc
     6361 ggttttcgtt tggcctgatg ggacgggcgg gctacgaaac atatcgcgaa cggtacgaaa
     6421 actaccaaaa aaagcgtcaa aaagtgacgg tttgagcacg ttttagcgca gtattctcgc
     6481 gggcagcagg aacagcgcgc gaatgaactc gaacaatccc gtcaccacga ggccaaccag
     6541 ccggaagggc agcaggagca gccacaccag cgggaagagg aacagcgcaa ggatggccag
     6601 cggccagcac agcaccaaca agatacagaa aagaaggaac ttaaccacca ccgcatcgcc
     6661 ccctccaggg cgtctatcga atactacggt agcgcacgca actccgttcc cgaaatcgga
     6721 agacccttac gggtggcaga tgtagtcggt ccggccgccg tcgacggtca gaatttgcgc
     6781 ggtgatgaag ctggcctcct ccgaaaccag aaaagcgacg gcgtgggcga tgtcctcggg
     6841 acggccggtg cggcgaacca tggctttgtt ggagatgccg gcgagcaact gctcgacctc
     6901 atcgggcgga cgatccgcgt acaccatgtc agtgacaatg aagccggggg cgatggcatt
     6961 gacggtgatg ccgtccgggc cgagttccag tgcgaagcga cgggtcagga cggcaacagc
     7021 ggccttggta gcagcgtaga aggtagttcc cgccatggtg gtgccgtggg ccgcgatgga
     7081 agtgagattc acgatgcggc cccagcgccg ggccttcatg ccctcagcga cggcgagggt
     7141 gacgttaacg accccatcga cattggtccg gcgcatggcc tcgagggcgg aggaggagaa
     7201 gtcatccagg tccccgcggc ggaacaggcc ggcgttgttg acgaggatgt cagtcgggcc
     7261 gagctccgac gcaacagtcc ggaccatttc gcgaaccgca gccggatcgc tgacgtcggc
     7321 ctgaatggcg aaagcgcgac caccttcgga gttgatctcc tcggcgagtt cctcggcttc
     7381 gcggcggcgc tcaaggtagt tgactgcgac ggcggcgcca ttccgggcga gccgcacgca
     7441 gatagcgctg ccgatgcccc gggaacctcc ggtgacgagg gcggtccggt ctttcaggct
     7501 catggtttcc cgattctctc acagcacagg aacactggct ctgcgtagtt tcgctcactg
     7561 tctatgatag tttcagatcg ttaactctga aaagagcgta gtttcaccca ctgtgatgaa
     7621 tcgcgaacgc tatccgattt gttttcaacg gattaagttt ggcaaaggag atgcacacgg
     7681 aagtaggtta gcggagcagt acgagcgggg actcgtatgg ctacaggcca gaggaagatg
     7741 ggcgcggtgg tgaacagtct ccgcgaaggg actcatacaa atgaaaaagc tgaagtcaga
     7801 aactgaaacc acgattctgc aacggcggca gcagccgctg gacgtctttt tctcgccgga
     7861 gaacgtggcc gtgatcggcg cgacggagac cccgggaagt gtcggccgca cagtgctatg
     7921 gaacctgatc agcacgccgt tcggcgggac ggtgtacccg gtaaacctga agcggtcgag
     7981 cgtgctgggc atcaaggcgt atccgaatat cggcgctgtt cccggggaag tggacctggc
     8041 ggtgatcatc accccggcgg cggcgatccc gggcgtgatc cgggattgcg cggcggctgg
     8101 agtgcagggc gcgatcgtga tttcggctgg gttcaaggaa ctcggcccgc agggcgccga
     8161 actcgagcgc caggtgcttg tggaagcgca gaagggcggg gtgcgaatta tcggtccgaa
     8221 ctgtctcggc gtgatgagcc cgccgacggg tctgaacgcg acgttcgccc gcggcatggc
     8281 gaggcccggc tcggtcggct tcatcagcca gagcggcgcg ctgtgcacgg cggtactcga
     8341 ctggagcttc cgcgaaatgg tcggcttcag tgcgttcctc tccatcgggg cgatggtgga
     8401 cgtcgggtgg ggcgacctga tctactacct cggcgaagat ccgaaaacga agagcatcct
     8461 gatttacatg gagtcgatcg gcgacgcccg gtcgttcctg tcggcggcgc gcgaggtggc
     8521 gctgaccaag ccgatcatcg tgatcaaggc cggacggacg gcggcgggcg gcaaggcggc
     8581 ggcctcgcac accggttcga tgacgggctc ggacgatgtg ctcgaggcgg cgttccgccg
     8641 cagcggcgtg ctccgggtga accagatctc ggacctgttc tacatggccg aagtgctggc
     8701 gaagcagccg cctccgaagg gcccgaagct gacgattctc acgaacgccg gcggtccggc
     8761 ggtgctggcc accgacgcgt tgatcaccag cggcggcgag ctgaccgagc tgtccccgga
     8821 aaccttcgag gagttcaaca ggattcttcc gccgacctgg agccggaaca atccggtcga
     8881 catcatcggc gacgcgacgc cggaacggta cgccaaagcg ctggccacgg cggcgaaaga
     8941 cccgaacgcg gacggcatgc tggtggtgct gacgccccag gacatgaccg acccgactca
     9001 gatcgccgag caactccggc cgtacgccaa catgggcggc aagccggtgc tggcgagctg
     9061 gatgggaggg ccggaagtgg cggctggcga gcgcattctt aacgcggccg gtatcccgac
     9121 gttttcgtac cccgataccg ccgcgcgcat gttcaactac atgtggcagt acagtgaaaa
     9181 cctgcgggcg ctctacgaga cgccggtgtt tccggcggga gcaccggagg tcgaggtcag
     9241 ccgcggaaaa gccgagacta tcattgccgg cgcacgcgcg gagggccgga cgattcttac
     9301 cgagttcgaa tccaaacagg tgctggcggc atacggcatc ccgacggtgg agacgcgcgt
     9361 cgcccggagc gagcaggagg cagtggcagc ggccgacgcc attggctacc cggtagtgtt
     9421 gaagctgttc tcggaaacga tcacgcataa gaccgacgtc ggcggtgtgc agctgaacct
     9481 caaggatgcc ggagcggtcc gcgaggcgtt ccgacgcatc cagacttcgg tcgcggagaa
     9541 agccggcgcc gagcatttcc tgggtgtaac cgtgcagccg atgatcaagc tggacggtta
     9601 cgagatcatc ctaggcagca gtcttgaccc gcagttcggc ccggtgattc tgttcggcct
     9661 cggaggacag cttgtcgagg tattcaagga ccgggcgctg gcgctgccgc cgctgaacac
     9721 cacgctagcg cggcggatga tggaacggac gaagatctac acggcactga aaggcgtccg
     9781 cggccgcaaa ccggtggatc tggccgcgtt agaacagttg atggtggcct tcagccgcct
     9841 ggtggtggag cagcggtggg tgaaagagat cgacatcaac ccaatgcttg cgtcaccgga
     9901 gcggctgatc gctctcgacg cgcgggtggt ggtgcatgag ccgacggttc aggagagcga
     9961 tcttccgcgc ctgccgatcc ggccttaccc gacccgatac gtgtcgccgt ggacgacgag
    10021 ggacggcagt gacgtcctca tccgcccgat ccggcccgag gacgaaccgc tgatcgtcaa
    10081 gtttcacgag acgttgtctg agcggacagt ctacctgcgg tacctgcata tgatgaaact
    10141 cagccagcga gtggcccacg agcggctggt agggatctgc tttatcgatt acgaccgcga
    10201 aattgcgctg gtggcggagc agaaggatcc gaagaccggc gagccgcgca ttctggcggt
    10261 cggccggctg cggaagctcc atggcaccaa cgacggcgag ttcgccgtcc tcgtgagcga
    10321 cgagtaccag ggccgcggcc tgggcatcga gctcgtgcgc cggctgatcg aggtggctcg
    10381 cgccgagaag ctcagtgcga ttgtcggcga cgtcttgcag gacaacgcgg tcatgcggca
    10441 catctgcgag acgcttggtt tcacgatgga agggtcgatc gaagatccag tgttgaaggc
    10501 ggagtacaag ctgtagtagt gtggggcagg tcgagacctg ccccacgcgc gacaacccaa
    10561 cggttgtggg gaagggagag gaatgctggt cattggttgc ggaaatggag accgtggcga
    10621 cgatgctgcc ggaatcctgg tagcgcgccg cctgcgcgca caaggcatcg aggcacgcga
    10681 gcagagcggc gaagcgctgg cgctgatgga gagttggcgg gacgcaccgg gtgagcagga
    10741 agtcattctt gtcgatgcgg tggtaacggg ggcgcctcct gggacggtta ccgtatggga
    10801 cgcacgcacg gcgcccgtga tcggcgactt tttccgctgc tcgacgcacg cattcggcgt
    10861 cgccgaggcg atcgagctag cgcgcattct ggaccttctc ccgccgaacc tgctcatcta
    10921 cggcatcgag gccggcagtt tcgatatcgg cagcgaacct tctccggagg tcctggcggc
    10981 agtggagcaa gtgactgacc gcatcgggtc ggagatcggc gtcgcccccg ggcgaaactg
    11041 agcgattccg cacccctcag ttattctggt tgtatatgca cgaactggcg ctcgcccaga
    11101 gcattctcga gatctgtgaa gaagaggcga gaaagcggca agcgggcgcg atccacaaga
    11161 tcaagcttcg cctgggcgat ttttccggag tggtccgtga agcgctggag ttctctttcg
    11221 atgcgctgaa agtggagacg ctcgccgccg gcgccggtct cgaaatcgaa cgggtaccgc
    11281 ttgaagtgcg ttgcccgcgc tgcgggcagg ttagcagccc gcccgcctcg gatttgtgtt
    11341 tcgtgtgcac cgaatgcggc agtccggtgg aaatcacaag cgggcgggag ctccaggtcg
    11401 agtacatcga ggtggagtaa agaggagcgg gagccaaacg gaaggagcgg atgatggaac
    11461 gagtgccggt ggtgaggaac gtgttgcagg ccaacgagga agcggcggcg gcgaaccggg
    11521 agctcttcga tcgcgggggc atgctggcga ttaacctgat gtcggctccg ggcgcgggca
    11581 agacgtcggt actcgagcag accatccggc gcctgggcac tcgttaccgc ctcgccgtga
    11641 tcgagggcga ccttcagacg agcatcgacg ccgagcggat ccgggcggcg ggcgtaccgt
    11701 cccaccagat cacaaccggc acggtctgcc acttggacgc tcgcatggtg tcaaaggcag
    11761 tggcggagtt cccgatggac ggcgtgcgcg tgttgttcat cgagaacgta ggcaaccttg
    11821 tctgcccggc ggagttttac ctcggagaac acctgcgggt aatggtatac agcgtcgtcg
    11881 agggtgcgga gaagccgaag aagtatccgc tgatgtttca cacctccgac gtggtactgc
    11941 tgaacaaggt ggaccttgtg ccttacgcgg gtgtcgagat tgaggacctg aagaagaatg
    12001 ttcttgaagt gaacccgagg gcgacgattt tccccgtctc ctgccggaca ggagaagggc
    12061 tggatgcctg gaccgagtgg ctggactcgg cggttcgcga gtcggcggcc actgcctaga
    12121 gctcggggat cacgaggacg tcgccgggat ggatcacggt cttgtcgtcc ttcagcttgc
    12181 ccgggttcgc cttgatgatt tcagggtata ggtttccctt acccttgccc cattgttttt
    12241 cggcgatctt ccagagcgag tcgcctgcca ccaccgtgta cgtcttcggc gcgggcgcaa
    12301 ggctggcgtc gatcttcaga tcgcaggtca gatccgaata gctcgcatcg accagcttga
    12361 tttggttcca gacgtcgttc tttaccgcct ctgagggcgc cgctccctgg atgagcagct
    12421 tgtctttctc cacgtgcacg tgcgacagcc tcaccccctt ctgtatggcg aggttgatta
    12481 cggactggta tttcagcttc agttgatcta gcttgtccat cccccaatga tatcgttcgg
    12541 ggcgggtgtt agcatggctg agggaggaag gcacatgcaa gagctactca acaccattct
    12601 ccaggcacag ggcggtggac tcgtcaagca actcgccggc cagttcggca tcgacgaatc
    12661 ccaagcgggc tccgcgcttg gccagttgct cccggcgctc accggcgctg cccaaaacaa
    12721 cgttcgccag ggcggtcttg acggactgct tggcgctctg tccagcggcg gccacgagcg
    12781 ctacatcgag gacccctcct tgctgggcag ccaggagacc gtcgaggacg gtaacggcat
    12841 ccttggccac ctgctcggaa gtaaggacgc cagccgcgcc ctcgccagcc aagtttccgg
    12901 ttcaactggg ctgggtgtgg acgtgctcaa gaagatgctc cccgtcgtcg ccaccctggt
    12961 catgggttcc atgagcaagg ccacgaatca gggaagcacc ggcagcggcc tcctttcgat
    13021 gctggacacg aaccgtgacg gcagcgtcct tgacgatgtc gcgggcttca tgggcaagtt
    13081 cctaaccaaa cgcatttgaa atcttgattc caccggtcgc cgggccgatg atcgagttgt
    13141 cgagacaaga tcggaggacc gacgaccggt ggaacttttc gcaaagctgg cccaaacagc
    13201 aagctggaag ccgcgtctca caaggctgct ctgctcgcag ccatggcgtt tcgaacctcc
    13261 agcgaaagct tggctcccag aatctctgtc ttctctatgg aaggcggcac ggccaagaga
    13321 tgcggggcgt gggccatcag tgctttggca atgggcccgt tgaggtgagc ttgccttccc
    13381 gactcatcgg gaaacgcatc gactatggcg aacgtggatg ggcccaggcg cagcgcgaac
    13441 cacagcggag tggtcgtttc ctcatttgcc agttgaagac cttgtctcag gaaggcggcg
    13501 acctcttctt ccttcccagg tttcgcctca agtcgtacaa acaacccaac tgaaagcatt
    13561 tccaattctc cttaggccga agtgtcggcc tgttcccaga ctacgtccga gttcgtcttt
    13621 atactagagg caagaatgcc atatttggta tcattattgc catgagagta aacgtcctgg
    13681 ttctggatgg agtttttgac ctgggattat ctgctgtgct ggacgcgttc caaactgcga
    13741 atgagttgat cggaatctcg gggcttacgg tgcctcgctt cgaggtgaag atcgccgggg
    13801 tccgcaaggc ggtcaagacg tcgcagggtt tgacggtgcc ggtaaacgca acgggttggc
    13861 gcacgcccaa ttgcgttgtg gttccggcaa ttgggttcaa gatgccgggc ccgttggaga
    13921 gtgcacttgc aaggccggac gtgcaagatg cgggcgcttc gcttcggcaa tgggctcgcc
    13981 tcggcgcaac gatgaccgca gcctgcattg gaacctttgt gatggccgag tccggactgc
    14041 tcgatcatca tcacgcaacc acaacatggt ggctggcgcc gctctttcgg aagcgatatc
    14101 cccaggtcct gctggatgaa tcgaatatga ttgtgaaatc cggcaagttt gttacggccg
    14161 gagcggctct cagtcacatg gatctcgcgt tgtggctggt gcgaggagtg agcccgacgc
    14221 tggcttcttt gacggcaaaa tatctgatcg tcgattccag gccatcacag tcggcctatg
    14281 cattgacgga tcatctggtg cattccgatc ctctgattca gcgcttcgaa agctgggcgc
    14341 gctcccggtt gaagcacgga ttttctttgg acgacgcagc agaggccgtc ggttcgagca
    14401 agcgaacgct cgcgcgccgc atgcagacag tgctcggaaa gtcgccgctg tcttacttcc
    14461 aaagcttgcg cgtggagcgc gcggtgcatc ttctgaagac cagcaacgcg agcgtcgacg
    14521 agatcgcgga gcgtgtcgga tacacggaag gctcgattct gcgagttctg ctgcggcgac
    14581 gtcttggcct cggaatcaag gagatcaggc ggtccgcctg atcctttaag gttgcaggcc
    14641 aacgacttcc gttgacatgg ggtaggtttt caacaagccg gggcttccgc tcccctcgat
    14701 cgtgactctg cttgctaact cgggactgcg ttaagccgcc aaaagtgttt ccgctctggt
    14761 tgtgcgtaat tcctccacgc tcggtgctgc aaaatcaggc agtactgtga aatttccact
    14821 caattccgcc gggttatagt ctgcagttaa ttccaacagg tgaaatagat cactctctac
    14881 gaaagttata gggagtttat aacgactgcg acaggtagga cgttgaatct tcacaagggc
    14941 gatggggaat caggctccga atcacactct ggcacaaggg cttcaggata cttccggata
    15001 ttggcacccg atttgcctca accattccga caagcaaaag atcgggcctt ccggcacaac
    15061 catcttcgtg cccggtccag ttgtgtaaat cctatttcac ccccatgggg aggaacacat
    15121 gacgatttct cgtcgacaat tccttgagtt gtttaggaat tccgcggcag cactcggcgt
    15181 gggcgcagtg aatctgtcgg ttcttgagaa ggcgctcgca caaccaggtg gtccgacggt
    15241 catctggctc caaggcagcg cctgcaccgg atgctcggtc tcgctgttga acagaatctc
    15301 gagcacttcg ccgagcacca ccgcggacgt gcttatcaat atcatcaacc tccgctatca
    15361 cccgaacctg atggcggcag ccggaaagtc ggcggctgcc atcgcagagg agatcgccgg
    15421 cggaagtgga cacattctgg cggttgaggg aggtgtgccg acggcgttcg gcggcgccgc
    15481 gtgctgggct tggacgcgtg cggacggcac cgacgtcacg ttccagcaag cagtgacaat
    15541 gctggcagcg aatgccgccc aaatcctgtg catcggaaca tgcgcagcct ggggcggtat
    15601 cccggcggcc cttccgaatc cgacaggcgt caggagcgtc cgagaggtca ctggccgccc
    15661 gactctgaac atcgcgggtt gcccgacgca cccggactgg atcgtttggg ccatcgggca
    15721 attggtgtcg ggcgccccga tcagcgtgga ctcgtacgga cggccggctg cgctgttcgg
    15781 caagacagtg cacgagctat gcccgcgccg ggagcgggag gaagcctcga cctacggaca
    15841 ggattacctc tgcctggaag aacttggctg caaaggcccg tccaccaagg gcaactgccc
    15901 gatcactttg tggaacggca gagccaactg gtgctgtgac tccaacacgc tgtgcatcgg
    15961 gtgcacggac ccagcttttc cattcacaaa cctgaggagc aaaacctatg ccatcgacga
    16021 ttaagattga ccccgtaacc cggctggaag gccacctcaa aatcgaagtc acagtcgacg
    16081 gcgtagcggg cgcacagaag gtcgtggacg ccaaggcgac cggcacgcta ttccgcggct
    16141 tcgaggcgat cctgaagaac cgcaatcctc tggacgctcc cgacatcacg cagcgaatct
    16201 gtggcgtctg cccggtgtcg cacggcctgg cagcttcgat gacgctcgaa aacgcggccg
    16261 gggtaacggc gccggataac gccaggatca tgcggaatct ggtactcggc tccaacttcg
    16321 tgcagtcaca catcctgcac ttctaccatc tcgccgtgca ggactttgtc agcggtcccg
    16381 atatgccgcc atggcagccg aactgggctg ccgacaagcg attcgatgcg gctacgtccg
    16441 gcgtcctagt cagtcactat gtccaagcgc tcgaaatgcg acgcaaggca cacgagatgg
    16501 gagcactgtt cggagggcgc ttaccgcatc ctccagcgtt cgtccccgga ggcttcacca
    16561 ctactccccg tccggaacga atcacgaaat tcagggccta cctcgccgag ctgatcgcgt
    16621 tcatcaacaa cgtctatctt ccggatgtcg gagctctcgc caatgcctac gcggactact
    16681 actccatcgg gcggggtcac ggcaacctgc tggcatacgg cgtgttcgac ctggacggcg
    16741 ccgggcagag caagttgctc cgccggggga ggatccttgg aggcaccgga tcggcactgg
    16801 cggtggaccc gaaggctatc actgagtcgg tcacatattc ctggtacagc gacaccacga
    16861 acaacctctc accgtcctcg ggcgtgaccc agcctgtgta cccaaaaggc ggcgcgtatt
    16921 cgtggctcaa ggcgccacgg tacggcgggg ccagcaggcg caagggcaag cgcgtcacgg
    16981 tgacaccggg agcgccgtac gagaccggcc cactcgcgag aatgtgggtg aacggcgagt
    17041 acacgcgcgg catctccgtg atggaccggc atgcgtcccg ggcgcaggag acgctcaaag
    17101 tcgcgcaggc gatggacgtc tgggcgaccc agttggggag cgggccggtg tatcagcagt
    17161 atgtgacgcc gaccagcgcg tccggattcg gactgaccga ggcgccacgc ggcgctctcg
    17221 gccactgggt tgatatcagc aacggcaaga tcggcaacta ccaggtgctt acaccgacgt
    17281 gctggaacgc atcgccgagg gacagcgccg gggtgcccgg gccgatcgag caggcgctgg
    17341 tcgggactcc ggtggcgaac ttggatgagc ccgtcgaagt gctgcgtgtg gtccattcct
    17401 tcgacccatg cctttcgtgc gcagtccacg tgatgcggcc ggccgagggc agtaagatct
    17461 tcgcgctggg acactatcac ggcgaagacg aggtccatga gcacacgcac tcgcactgac
    17521 ggaaacggcc cctccgcgcc gccgcggagg ggccgccgaa tccttgtcgc cgggctgggc
    17581 aacctgctgc tgatggatga cggtgttggg gtgcacgcag tccgcgagct gagacggcac
    17641 gccgggagtg gcgtcatggt ggcggaggtg ggcacggcgg tgctcgacgc ccttcacctg
    17701 atcgagaagg ccgaccggtt gcttgcgatc gacgcaatgc gggcgggagg cgctccgggc
    17761 acgatctact cattcggggt ggaggacgtc aagcagcggc ccacggaggc ctcgttacac
    17821 gaattgaacc tgttgtcggc gcttacgctg acgcggcgcc ggccgcgtga gatcactatt
    17881 ctcggcgtgg agccgggccg aatcgactac ggcctggaac tgacacccgc ggtgcaggaa
    17941 gcactcccac ggctcgttag cgcggccagc aggattgtcg cgaagtgggt gggcgggacg
    18001 ttcgggcgcg acgggtgacg cgtgttacca tctgaataac gacctgtttt ccacggattt
    18061 cttaggatct cgccttgaat gtgaccgttt catccgccga gcggctgcgc gtgaccctgc
    18121 atggagcggt gcagggcgtc ggtttccggc cctttgtcta ccggctggca actgaattgg
    18181 gactgccggg ctgggtactc aactcgagcg cggggctgtc actcgaagtg gagggtcctc
    18241 gcgaccggct ggacaccttc ctcgcgcgcc tcgaatcgga gaaaccgcca gcagcggtca
    18301 tcctgacgaa ggaggcgagt ttcctcgcac ccgtggggtt tacagggttc gaaatccgca
    18361 caagcgacgc aagcgaaggg aagaccgcaa gcgtactgcc cgatctcgcg acctgcggcg
    18421 actgcctggc ggagatgcgc gatcccgccg accgccgctg tggctatccg ttcaccaact
    18481 gcacgaactg cgggccgcgc tacacgatca ttctcgacat tccgtacgac cgcacaaaca
    18541 cgacgatgtt tgggttcggg ttgtgcgccg agtgcggccg ggaataccgc gatcccggcg
    18601 accggcggtt ccatgcacag ccggtggcgt gcccggcgtg cgggccgaag gtgtgggtgg
    18661 aattgccgca gggaatggag atgcagggga gaatggggac agacagccat ttcgcgacag
    18721 cggcgaaatg gaatctgtcc ccattttccc caattttcgt cgcggcggcg ctggagcggg
    18781 gggagatcgt cgcactgaag gggatcggcg gattccagtt gctcgcggac gcgcggaatg
    18841 agcaggccgt ggagcgcctg aggcagcgca agcaccgcga ggaaaaaccg ttcgcgctga
    18901 tgctgccgtc gatcgaggag gtgcggcggt actgcgaggt ctcgccggcg gaagagcggc
    18961 tgctcaagtc agcggcggcg cccattgtgc tacttagacc gaacggaacg cccggcatcg
    19021 cgccaagcgt agcgaagtct tcgccgtacc tcggagtgat gctcccctat tctccgctgc
    19081 accacttgct gatgcgcgag ttccccgccc cggttgtcgc caccagcggc aaccgcagcg
    19141 acgaaccgat cgcgatcgac aatgatgaag cacgggatcg ccttggcgaa atcgccgatg
    19201 tgtttctgat gcacgaccgt ccggtggcgc ggccgtgcga cgattccgtg gcgcgcgtca
    19261 ctcgaggccg cgagagcgtg gtgcggcggg cgcgcggata tgctccgctg ccggtgcgcg
    19321 tcgcgaggga attgccgccg gtactcgcgg tgggcggcca tttgaagaac acggttgcca
    19381 tcgcgatcgg ccgtgacgtc tacctgagcc agcacgtggg cgatctcgac acggcagagg
    19441 caaggggggc cttcgagagg gcgatcgaag acctttgccg gctctaccgt tttgagcctc
    19501 aaatggccgc ttgcgatctc catccggact acgcttcgac gcagtgggcg cgggcatcgg
    19561 ggctgcccgt gatcgcggtg caacatcacc atgcgcatgc cgcggcctgc gccgccgaga
    19621 acgacatccg cggggactac ctgggtgtgt cgtgggacgg aaccgggtac gggctagacg
    19681 cgactgtatg gggaggcgaa ttctttctgg tgagggacgc ggagttcgaa agagtggcgc
    19741 acctgcgacc cttccggctt ccggggggcg aggcggctat ccgcgaaggc tggcgtccgg
    19801 cggccagctt gctgtgggag gttttcgggc cggagggtgt gccggagtcg gcccagaagc
    19861 cggtcctgtt gcggatgctc gagcgcggcg tgaatgcgcc gctgacgaca agtgtcgggc
    19921 ggttgttcga cgcggtcgct tccatcgccg gcatcgcgca ggcgaaccgg ttcgagggcc
    19981 aggcggcgat gcaactggag cgcgcgatcg gggccaccgt gacagcggag tgttatcgtt
    20041 taggtgaagg gggcgactgg gcgccgttgg tggaagaggt aggggccgat gccgcccgga
    20101 atgtctcgcc ggccctgatc tccgcgaagt tccacaatgc gctggctcgc tggatcgtcg
    20161 aagtcgcgga gcagacgggg gtcggccaag tcgctctgag cggcggtgtt ttccagaaca
    20221 cctacctgac ggaacgtgcc gcctcgctgc tcgaggaacg cggcttccgc gtgtacacac
    20281 accagcgggc gccggcgaac gacggaggca tcgcactggg gcaggcggtg atcgcgggga
    20341 ttcgggcggg aggttgactt tatgtgtctg gccgtaccgg gaaagatcgt cagcgtaatg
    20401 gaggatgacc cgccggaagt gcgccgcggg aaggtggact tcgccggcgt gcagaaagag
    20461 gtctgccttg cctacacgcc ggaggcgaaa atcggagatt acgtgcttgt gcacgtcggc
    20521 ttcgcgctga acgtggtgga tgcggaagag gcgcagcgca tcttcgagac gctcgacgag
    20581 atgcagcggc tggaggagct ggccgaggcg gagagagcgg agagcgggcc atgaagtaca
    20641 tcgacgaata ccgtgacccg gagcgcgtcc ggcagcttgc ggccgccatc cggaggaaga
    20701 ttacccgccc gtggaccatt atggaggtct gcggcgggca gacgcacacc atcgtcaaga
    20761 gcggccttca ggaattactg cctgaggaag tgacactcgt gcacgggccg gggtgcccgg
    20821 tgtgcgtgac gccgctcgaa ctgatcgaca gcgcggtggc gatcgcctcg cggccggacg
    20881 taacgttctg ttcgttcggc gacatgctgc gcgtgccggg ctccacgaag agtctcttcg
    20941 acgtgaaagc ggagggcggc gacgtgcgca tcgtctactc gccgctggat gcgctgaagc
    21001 tggcgcgcgc gaatccgcaa cggcaggtcg tcttcttcgc ggtaggattt gaaacgaccg
    21061 caccggccaa cgcgatggcc gtctggcaag cggaacgcga aggtatcgcg aacttctcga
    21121 tcctggtggc gcatgtgctg gttccgccgg cgatcgaggc gatcctgagc tcgccgaact
    21181 gccgcgtgca ggcgttcctg gctgcgggcc acgtctgcac ggtgatgggc ttcgaggagt
    21241 acgagccggt ggcgaagcgc taccgtacgc ccatcgtcgt gacgggtttt gagccgctcg
    21301 acctgctcga aggtgtgcac atggcggtgg cacaactcga ggagggccgc gcggaggtgg
    21361 agaaccagta cagccgggcg gtgcgccgcg agggaaaccg gccggcgcga gaccgggtgg
    21421 ccgaggtctt cgaggtgggc ccacgtcagt ggcgcggcat cggcgagatt ccggcgagcg
    21481 gtctgcggct gcgggagcgg ttccgcaagc tcgacgcatt ggagcggttc gcgactggcg
    21541 tggggcatgt caaggagccg gcgatatgca ttgccgggct tgtactgcaa ggcctgaaga
    21601 agccgtacga atgcgcggcg ttcggcaagc agtgcacgcc ggagacgccg ctcggagctc
    21661 cgatggtgtc ctccgagggc gcctgcgccg cctattacct gtatgggagg catcgtcatg
    21721 acgccgttcg agctggcgtg tccagcgccg aagttcactg aagaccgcgt gctgctcgcg
    21781 cacggcggcg gcggccggct gatgcagcgg ctgatcgagc aggtgttcct gcctgagttc
    21841 cgcaacccgg ccctggaggc gctgcacgac ggcgcagtgg tggacgtgaa cggttcgcgg
    21901 ctggccttca ctaccgattc ctacgtcgtg cggccgctat ttttcccggg cggaaacatc
    21961 ggcgaactgg cggtctacgg gacggcgaac gacctcgcga tgtgcggcgc acgcccgctc
    22021 tacctgagcg ccgcgttcat cctcgaagag ggcttgccga tggaaacgct gcgggcggtg
    22081 gcggcttcga tgcgcgaagc ggcggcgcgg gcgggcgtga cgctggttac gggcgacacc
    22141 aaggtggtgg acaagggcaa gggcgaccag atgttcatca cgacggcggg cgtcggcacg
    22201 ctgtcagagg ccgtccggat cggaccggcg ttcgtcgagc cgggcgacgc ggtgatcgtg
    22261 tccggcgacc tcggaaggca cggcgtggct gtcctgtcgg tgcgcgaggg actcgagttc
    22321 gaggcgccgg tggaaagcga cgcggctccg ctatgggagc cggttgaggc gctgttgcgc
    22381 tcggggatcg aagtgcattg cctgcgcgac ctgacgcgcg gcgggctggc ctcagcgttg
    22441 aacgagatcg ccgggtcggg tggcgtcggc atcggcattg acgaggcatt ggtgccggtc
    22501 cgcgaagcgg tgaagggcgc ctgcgagatc ctcggtctcg acccgctcta cgtcgcgaac
    22561 gagggccgtt tcgcggcttt tgtgccgaag gcacaggccg aactggcggt cgagacgctg
    22621 cggatggtgg gggtgtcgga gggtgcggcg gtcatcggag aagtaagcgc ggagcatccg
    22681 cgaaccgtgg tactgcgcag ccggatcggc ggccaccgcg tgctcgacat gctgagcggg
    22741 gagcagttac cgcggatctg ctgaagatct gccgcttgct cacgcgcgcg gttcttctga
    22801 gcacgcagag actctcagtc agtggcctga gggattccgc ttgcgggttc gaggacagcc
    22861 gacatcgagc ccttcgattt cggcatgcgt gggtccgggc catagccgtg gatctggtcg
    22921 cgcgcgaact ccgcctgggg aagctcgcag gtgatcacga tcgtccgtcc ggtggtatcg
    22981 acctccaccg cgtgccggaa agcctcgaac tgagagagta gaaacaatcg ctgaagcatc
    23041 tcgatgacat agtcgtacgt gtggtcgtca tcgtcgagca gcacgacgtg gaagagcggc
    23101 atcagttgct cgcggcgggt ggtttctgtg tccggagaaa cggcgggtct cgacattcgc
    23161 gcgcgtcttc ttttcaagcg tactgtaaaa ccggccgttt tgtcttgacg tggaggccgg
    23221 cggcgcgcgg tgctacgatg gagatggaaa gggggcgtaa cggattcgac gggattgaat
    23281 tgtgatggag aggcgtgccg ggttccgagc tcccgttaaa cgatcggaaa cacacaactg
    23341 ccaacacgca gtttgcattc gctgcctaat tcgttaggcg gccgctccgg ctgctgagcc
    23401 cgcgcgggag ttagcgagcg tcattttgcg ggataggccg agggcttctg ctcgtagccg
    23461 caggctgaga tcaatcgagc taggctgtcg tcattctggc cgcttctgag gcggcagtcg
    23521 aatacaccga ggcggctacg cacgtaggct cttcatggca ggctttctcg gacgcgggtt
    23581 cgactcccgc cgcctccacc agaaacagat tcgtgttgcc taacgtcacg caacggtctg
    23641 aagttcaatg aagtcgacat cggaagtggg ctccgggatc ggttccccga cttcgcgaat
    23701 ggcttcaagg tgaagctcaa ttgcctcgcg gatgttgcgt ttcgtttctt cgagggtctt
    23761 tcccgtcgtg acgcaaccag gcaagtcggg gatgtaggct ccccagttgt tccccgcctt
    23821 ttcaaatacg accgcgtatc gcttcatggc tccccgatct ccgctttcct gagaatcgac
    23881 ttcagtgtcc cgacgggaag gtcatcattc cagttccccg gcacggtaac tacttaaccg
    23941 gatgcctgaa cgcccggtga cttcgcttct gccggaccaa tcgccactga ttcgattcca
    24001 gcagcctgat gacttcccga actttcatcc ttcagaggat tttaccgccg tccgtctcgc
    24061 ctctccattg aagcgtgaag acgtctggtg gaatcactgc ccgctcagcg gcacgcggca
    24121 ct
//
LOCUS       DATIFQ010000247        21144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1803520, whole genome shotgun sequence.
ACCESSION   DATIFQ010000247 DATIFQ010000000
VERSION     DATIFQ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 21144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21144
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803520"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(661..843)
                     /locus_tag="VLE22_11440"
     CDS             complement(661..843)
                     /locus_tag="VLE22_11440"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HSB15064.1"
                     /translation="MKKQRVVRGIPVEEGSGNVYADLGYADSESMLVKAQLAAKIAEI
                     IQRRAPTQAGAAEIWG"
     gene            complement(889..2010)
                     /locus_tag="VLE22_11445"
     CDS             complement(889..2010)
                     /locus_tag="VLE22_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HSB15065.1"
                     /translation="MAEATKTSGRLKTYLGYAAGVGKTYQMLEEGQQLKAKGVDVVIG
                     YFEPHGRQDTIARTEGLEIVPRQIIEYRGARFDEMDTPAILWRRPEVCLVDELAHTNV
                     PGSERAKRWEDVIVLLDAGIDIITTMNVQHLESLNDQVWQITGIRVRETLPDWVLKQA
                     DEVVMVDLPPAALLNRLARGAVYAPDKAERALQYFFKESNLVALREIALRQTAHEVDL
                     RQPQVPGVPDHDAPKAARAGPAAGPERILVHITPDANTAILLRRARRVADYLRADCLA
                     VHIAEAGDSKRSSAAEREALAKHLNFARHLHVETRVLDSEDAAEALVNFARSRKVTQI
                     YMARPRYSLAKRIAGNFIHRVVRLAGDMQVIIVAEHRGR"
     gene            complement(2013..3110)
                     /locus_tag="VLE22_11450"
     CDS             complement(2013..3110)
                     /locus_tag="VLE22_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSB15066.1"
                     /translation="MASNPDSRPDPDRLLRQIEFDEQRRRLGRLKVFLGYASGVGKSF
                     RMLDEGRRRKERGEDVIIGAVQSKLSPDLISLLDRFEIISQICADRGGAMDVPAIIAR
                     RPQVCLIDELAHRNPSGCGNAHRWQDVEQLLEAGIAIITAVNLNHIEELHDTTQQITG
                     KEVRETVPKAFLMHAEEIAIVDAPPEQILERVGVRSETDRLVTERRLSELREMALLLA
                     AEVVDRQLESYLEAHGIGHSIGAHEKILVCITPRANARLMIESGRRNAQRFHGELIVA
                     YVQQSSLSAADQELLESHLALARDASARVEVLEDGDPIEAILRFANQNGVTQIFIGHS
                     LRQDWWTRLFGGPVDRLIRQAEGIDVRIFPH"
     gene            complement(3118..4422)
                     /locus_tag="VLE22_11455"
     CDS             complement(3118..4422)
                     /locus_tag="VLE22_11455"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB15067.1"
                     /translation="MTAVRLAVMIVVASAALAQTASEAELLNRIRQLEARLAAVEAKL
                     NASAIPSQPAQRASAEPSPGPPQISVNAYLDGYFSYNFSRPLGRINLLRAYDVTSNNF
                     SLNQATLVLERPPDTGSGRRFGIRLDLMYGQGTETLQGGAQNEPRPQVFRPVFQAYGT
                     YVFPLGNGLRIDFGKWASSLGFEGNYAKDQINYSRSYWFNFLPFYHFGFRSSYQVNDR
                     LILTHWLVNGANQTEDFNGFKSQHLLLSWKPASSVTWSIGYYAGREQRDLEPLLNPGL
                     PALPTQPGLSTALFRPVPRGRLHILDSYAAWDATPRLTLVGEADYVVNRVEAVSPPAL
                     VYGGAGYARYQFTPKFSLGMRFGWLRDRAGLFSGTDQLLKDTTLTTTYQVDTGFQLRA
                     EIRRDWSNMPFFLTAEPGILKPDQATATLGLIWWIGSKEGGW"
     gene            complement(4419..4985)
                     /gene="kdpC"
                     /locus_tag="VLE22_11460"
     CDS             complement(4419..4985)
                     /gene="kdpC"
                     /locus_tag="VLE22_11460"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HSB15068.1"
                     /translation="MTAVRMILLMTLLTGLLYPALVTAICQAVFPRQANGSLVERNGR
                     VVGSRLIGQPFTRPEYFHGRPSAAGEGYDAALSGGSNLGMTSRKLVERVRAFIDEFQK
                     QNPEHRGPVPADMVTASGSGLDPHISPAAARAQAPRVARARGVSVEQVLLLTRQFTVE
                     SAVGVLGDPAVNVLELNLALDQDFPVRK"
     gene            complement(4996..7092)
                     /gene="kdpB"
                     /locus_tag="VLE22_11465"
     CDS             complement(4996..7092)
                     /gene="kdpB"
                     /locus_tag="VLE22_11465"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HSB15069.1"
                     /translation="MTTGDTDLPSLLPKRLAHSRPLWDTRILKRAARESFAKLNPARL
                     IRNPVIFVVEAGATLVTAQLIRDAVSNSGVAGFSLQIALWLWFTVLFANFAEAMAEAR
                     GKAQADALRKTKTDAVAKRVSDSGRVERVAASRLRAGDVVLCEAGDIIAGDGEVIQGI
                     ATVDESVITGESAPVIRESGGDRSAVTGGTKVLSDNIRIRITSNPGETFLDRMIALVE
                     GAERQKTPNEIALNILIAGLTLIFLLAVATLHPFAAYSVIAAGAGKTPTVAVLTALLV
                     CLIPTTIGGLLSAIGIAGMDRVMQHNVLAMSGRAVEASGNVNTLLLDKTGTITMGNRQ
                     AVEFTPADGVTAAELADAAQLASLADETPEGRSIVVLAKQQYRLRGRDVAEHQAHFLP
                     FSAYTRMSGVDLDGRSLRKGATDAIGAFVRQFGGAVPAGFTETAERISRSGGTPLAVA
                     DCSRVLGLVHLKDIVKGGLRERIAQLRAMGIRSVMITGDNPLTAAAIAQEAGVDDFLA
                     QATPKDKLEYIRKEQAAGRLVAMTGDGTNDAPALAQADVGLAMNTGTMAAKEAGNMVD
                     LDSNPTKLIEVVAIGKQLLITRGALTTFSIANDVAKYFAIIPAMFMGTYPSLAALNIM
                     GLHSPQSAVLSAVIFNALIIVALVPLALRGVKYRPASAAALLRRNLLIYGLGGLIAPF
                     PGIWLIDQLLYFLRLA"
     gene            complement(7095..8843)
                     /gene="kdpA"
                     /locus_tag="VLE22_11470"
     CDS             complement(7095..8843)
                     /gene="kdpA"
                     /locus_tag="VLE22_11470"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HSB15070.1"
                     /translation="MSQAGVVQIALYFLILILVVRPMGTYMAQVFQGQRTFLHPVLRP
                     LEGLIYRLSGVGERAEQRWTQYAGSLLAFSAASFVLLYALQRLQGVLPLNPMGFGEKQ
                     VAPDLAFNTAVSFMTNTNWQAYGGETTLSYFVQMAGLAVQNFVSAAAGMAVAVALVRG
                     FARERSEHIGNFWVDLTRATVYVLLPLSIVAALFLCSQGVIQNFKPYVVAETIEGPRQ
                     TIAQGPVASQEAIKELGTNGGGFFNANSAHPYENPTPLTNFVEMLLIFLIPGGLTYTF
                     GRMAGDTRQGWALFAAMSVLFLAGAFVAYRAEASGNPMLAALGADARAGNMEGKEVRF
                     GIAATALFATVTTGASCGAVNGMHDSFTPLGGLVPLFNIQAGEVIFGGVGSGLYGMLL
                     FAILAVFIAGLMVGRTPEYLCKKIEQKEVKMAMLALIATAASILLLTSASSVLDFEKN
                     GYWNPPGPATANLNNAGPHGFSEILYAYSSGTGNNGSAFAGLNANTPWYNLTIGLAML
                     AGRFLMIIPLLAVAGSLAGKKRLPLSAGTLPTHGPLFVGLLAGTVIIVGALTFFPALA
                     LGPVVEHFLMHSGRLF"
     gene            complement(9032..9487)
                     /locus_tag="VLE22_11475"
     CDS             complement(9032..9487)
                     /locus_tag="VLE22_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15071.1"
                     /translation="MNAGVKRRGEQPQAAERHDRELRVNVVFTDLAETLLALRAAARL
                     AADLRAHIVVVVPQVVPYPAPIEAPPISTAFLVHRFRTVADDMAIDLHVRVYICRDRR
                     TVLAVALGPQSLVVIAGRRSWWPTREQRLAAELAHLGHDVVFCTHEWGS"
     gene            complement(9545..10258)
                     /locus_tag="VLE22_11480"
     CDS             complement(9545..10258)
                     /locus_tag="VLE22_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB15072.1"
                     /translation="MTSARILVVDDEPQIRRVMRTTLVAQGYQVDDARTGEEALEKLR
                     AAKFDLVLLDLNMPGIGGLATCRLIRDTSDAAIVVLSVRSSEKDKVDALDAGADDYVT
                     KPFGTPELLARIRAALRRVPLAGDPVHRVIELAGLEINLGTRRVAKEGKEVRLTPKEF
                     DLLHYLVTNPNVPIPHGKLLQAVWGPDYGQEVEYLRVFINQLRKKIEPEPSKPRYILT
                     EPWLGYRFVMPSRSDLENP"
     gene            complement(10255..11718)
                     /locus_tag="VLE22_11485"
     CDS             complement(10255..11718)
                     /locus_tag="VLE22_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB15073.1"
                     /translation="MAANDTHRKGVRMASYAGALAIIGLLAAVYFLFLPVNATTVALT
                     FLLAVLAVATTWGLGQAVTASFAAMFCFNYFFLPPMGTLTIEDPQNWVALAAFLVTAI
                     VASQLSAGIRKRATEAIQRRAEMERLYTLSRAMMLAETGPEAAKRVAGLIVQVFESPA
                     VMIYDKSRRSFHRAGPQDVPVTESRLQDVALQDTPFHDAATNTSLVPMRLGGEPLGAM
                     AISVTEISDTALNAVANLAAIALERARSLEAASQAEAARQSEQLKSTLLDALAHEFKT
                     PLTAIKAAATGLLTDAELKEPQVELLTVIDEETDRLNSMVTDAIRVARVEAGDVKVEK
                     QPTQIAELIDRIVRRLPGREEREISVHGPPDLPLVAADRELIPVVIGHLLENALKYSP
                     AGSPVSITARADGGAVMVTVSDTGPGIAPEDRERVFQRFYRGPWARTQSSGTGMGLAI
                     AREIVTLHGGRIWVEDSPGPGARVSFSLPIIEDGVTE"
     gene            complement(12775..13926)
                     /gene="ltrA"
                     /locus_tag="VLE22_11490"
     CDS             complement(12775..13926)
                     /gene="ltrA"
                     /locus_tag="VLE22_11490"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HSB15074.1"
                     /translation="MTVHELVGYLKQQWPAIREQLLSGAYVPQPVKRVEIPKPDGGVR
                     KLGIPTVLDRFIQQAVMQVLQRRWDRTFSDHSYGFRPGRSAHQAVEQAQQYIAAGYRW
                     CVDLDLEKFFDRVSHDKLMAKIAERVSDKRMLKLIRAFLTAGVMESGLVSPVDEGTPQ
                     GGPLSPLLSNIVLDEFDRELERRGLRFARYADDSNIYVRSRRAGERVMASITRFITTK
                     LKLKVNQQKSAVARPWERKFLGFSFTANREPKRRIAPKAVLRFKVKLRELTSRTRGVS
                     IERMAEELARYLRGWIGYFGRCQTPSVLEDLEKWFRRRLRSAIWKQWTRGSVRFTELR
                     KRNVGKDLAAQTAGSAHGPWRLANSPALAFALPNAYFDSLGIPRLTAGR"
     gene            complement(13923..14123)
                     /locus_tag="VLE22_11495"
     CDS             complement(13923..14123)
                     /locus_tag="VLE22_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15075.1"
                     /translation="MLAFLAEDTGEAPSAAEEGTESLAAKRKAESPAIGEQLMEEVCE
                     RENCKQALARIKANKGSPAWTE"
     gene            complement(14129..14791)
                     /locus_tag="VLE22_11500"
     CDS             complement(14129..14791)
                     /locus_tag="VLE22_11500"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HSB15076.1"
                     /translation="MAELNDEYIAKMEDVLETYEKPYDPKAPVVCLDEKPVTLHADVR
                     PASPAEPGREARRDNEYQRRGTANVFCAVEPKAGRHFTFPTPDRSAFEFAQVACRLAM
                     QYPDAETIHLVMDNLNIHCCKSLTDVFGVEVGSEIWDQFMVHFTPTHGSWLNQAEIEI
                     GIFSRQCLGTRRIPDLQTLRRESRAWNRRMNRDRIKINWKFDRRAARRKFGYKRKSFK
                     RS"
     gene            14778..15176
                     /locus_tag="VLE22_11505"
     CDS             14778..15176
                     /locus_tag="VLE22_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15077.1"
                     /translation="MSSATHHIFFPPRLEVVVEEQNPNRFPSHAWNQPPFYGFLRNQS
                     HGPAGAAFGRVAAHHGDDPLLLAVFQHSRRAGARLVIESGFKTSLLVTMADLPNGLRS
                     EWDHAGNPRRTDTLGQLQEGQGSQDDTDLL"
     gene            complement(15333..17438)
                     /locus_tag="VLE22_11510"
     CDS             complement(15333..17438)
                     /locus_tag="VLE22_11510"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSB15078.1"
                     /translation="MERRLICTFGCVALLLAVVFTLASCMTRPEPVAPKKQVSASRPS
                     WLHTTPLVMVSNHDSMPIFQRRRGGSPVGQEEEYIKGDSEEAIRKLKDIGVTLVILHF
                     YKGFGLEAEKDHMEKAKQTAALVHKYGMRVGVYVGSTIAYETFLAEKPQAEKWFVPDF
                     LGRPVFYDDQTFRKRVYFMHPEYREYMKRVLTIAEKEFKADLIHFDNTSMQAEPPIFQ
                     HPMAIDDFRKFLQTKYSPEQLKQRFGFSEVRYMLPPRYDRPLTAINDPLFQEWTDFRC
                     VQLTAYYAELEELLRTLNPEVAVETNPHSGISGRNTIWEQGVDYPRLLAHMDAVWTEE
                     GDPAGVTEDGILVSKIRTFKMASILKNTLFVANGGGGGSTLQLAESMAFNRQCLGDVG
                     GTLAGYDLPQDQRDYINFYRRNFDHYRDVDNIADVAVLHSYASMGYNNDRPAVSTMLF
                     EQALIQARIPFDLIFDDHLKDLSKYRVLVLADQECLNDEKLDVIRKFVEQGGGLVATE
                     LSTLFTEWRLRRPAFGLQDLFHAESPRLVRRGQVAVQETPPVRNQAGKGRVVYISDVK
                     PSIPKPPGARMTSQYWKLPENAKDLIDAVQWAAGARMSLEVKAPPNVVAELMEQKPAG
                     ELLIHLVNYGRVKTPSVSNIEVSVRIPQGKKASKVTLMSPDGGEDRQVNFVAKEDRIT
                     FTVPRLQTYTLALVTLAPR"
     gene            complement(17444..19426)
                     /locus_tag="VLE22_11515"
     CDS             complement(17444..19426)
                     /locus_tag="VLE22_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HSB15079.1"
                     /translation="MTPGWVNDEPLVVVGNWDSMPIFRRRVGGSSTSQEEEYRKEMTE
                     DAVKRLKDLGVNLAITHFYKGFGLQAEKEHLEDTRKYIALLKKYGVRAGVYIGSTVAY
                     ETFLAENPKAEDWFVPDFLGRPVFYDDQTFRKRVYFMHPGYREYMKRVLRLAVEDYKV
                     DEIDFDNTSMQAQPPIFEHPMAVQDFRNWLQSRYPQEALVKRFGFSNMRYVLPPRYDR
                     PLRAINDPLFQEWADFRCYQLSSYYAELARHIRHMNPNAAIATNPHSGISGRNTVWEQ
                     GVDYPTFLPHMDIVWTEEGNEATVTPDGILVSKIRTYKMATSLKKRVITYTAGTRGGK
                     IQMAESMAYNRQSLGMVGGALAGYQLPEDQRKYIRFFIDNFQHFRDVDNVADVAVLQS
                     HASMAFNNDRPWQSAMLFEQALIQAKAPFDIIFDQQLNDLGKYRVLVLPDQECLSDAE
                     LDLIRKFVSRGGGLVATEHSSLYTEWRRRRPDFGLKELFRVSAPPLEEGATPDAIMKG
                     PAVKGQAGNGRVVYIPEVKPAMLKPPSVSMTSEYWKLPVNAEELIAAVRWAAGGNLSL
                     DVKAPPTVTAELLRQEKTGAMVVHLINYDALRHPVVNGVVVSVQIPDGKKVERISLRS
                     PDEDKSLTIAPRLKGQRAEFTVPRLSTYSLVVINLG"
BASE COUNT         3977 a         6619 c         6361 g         4187 t
ORIGIN      
        1 acgctggagt tcctccagca tcattcttcg caatggggtc acaaggacct cctttccttg
       61 tgacccaaac tattccactc aacacgggaa tgctgctctc tttacgcacg cgtagcgtcc
      121 gccgcgcgag cggcttcgtt caaacggcgt agtgtagacc gaagtcgctc aactccaaga
      181 tggtcagccg ctcggtgaca cagccttcag ccacacgatc ccaaagtggg caagccgtaa
      241 ccggggcttt cgctccttcg ggcagaattc agcccgcgaa cataagtcca cactcaacgg
      301 ggggtcgaaa aaactgtgga gatgcgaaac gccgcgcgcc ctcgtgcgcc gcttgctgaa
      361 cgcctgctgc gccatccaga tgaaatcagg cgcggcgagg tgcgcgtctt cctgacggaa
      421 tcacagcgcg tcgctggcct gcggcgaggg ggcgatggag gtgcctccac agttcgcgcg
      481 gccattttcc aggcgaacct atgccacaca aagcgtcagc cggccgttcg aacgactcct
      541 gggcgtcggc tttatgacaa tgtggacatc ccgaccccag ccgggtcagg cattccaaca
      601 ggcggtgctc cgaaatcccg cgaaaccgcc ctttcaagag ggcggaaacc ttgggctgtg
      661 ttaaccccag atctctgcag cgccggcttg cgtgggcgcc cggcgctgga tgatctcggc
      721 gatcttggcg gccagttggg ccttgaccag catgctctcg ctgtccgcat agccgaggtc
      781 ggcgtacacg ttaccggagc cttcctcgac aggaatgccc cgtacgactc gctgtttctt
      841 catcgcgata gctccttggc gatcgctcca ccgccacgac gcgatatgtc agcggccgcg
      901 gtgctcggcc acgatgatca cctgcatgtc gccggccagg cgaacaaccc ggtgaatgaa
      961 attgcccgcg attcgcttcg ccagcgagta gcgcgggcgc gccatgtaga tctgggtaac
     1021 cttccgggac cgcgcgaaat tcacgagcgc ttcagcggca tcctcgctgt cgagaactcg
     1081 agtctcgacg tgaaggtgtc gggcgaagtt caagtgcttt gccagcgcct cgcgttcggc
     1141 ggctgaggag cgcttcgagt cgccggcctc ggcgatgtga accgccaggc aatccgctcg
     1201 cagataatcc gccaccctgc gggcgcgccg cagcagaatg gccgtgttcg catccggtgt
     1261 gatatggacc agaatccgtt cgggcccggc agccgggccg gcgcgggccg ccttcggagc
     1321 gtcgtggtcc ggcaccccag gcacctgcgg ctggcgcagg tccacctcgt gggctgtctg
     1381 gcgaagcgcg atttcgcgaa gcgccacgag gttggattcc ttgaagaagt actgtagcgc
     1441 gcgctcggcc ttatcgggcg cgtaaacggc gccccgggcc aggcggttca gaagagccgc
     1501 cggcggcaga tctaccatca cgacctcgtc cgcctgtttc agaacccaat ccggaagagt
     1561 ctcgcgaacg cggatgcccg tgatctgcca tacctggtcg ttaaggctct ccagatgctg
     1621 tacgttcata gtcgtgatga tgtcgatgcc ggcatcgaga aggacgatta cgtcctccca
     1681 tcgcttggcc cgttccgagc cgggaacatt agtgtgcgcc agctcatcga cgaggcagac
     1741 ttcgggtctc cgccaaagga tcgcgggcgt atccatctca tcgaatctcg ccccgcgata
     1801 ctcgatgatc tggcgtggca cgatttcgag cccctctgtc cgcgcgatcg tgtcttggcg
     1861 cccgtggggt tcgaagtacc cgattaccac gtcgacgccc ttggccttga gttgctgccc
     1921 ttcttcgagc atctgatagg tcttgccgac accggccgcg taacccaggt aggtcttgag
     1981 ccgcccactg gttttggtcg cttcggccat ctctagtgcg ggaagatcct gacgtcgatg
     2041 ccctccgcct gacggatcag gcggtcgacc ggtcccccga acaggcgggt ccaccagtcc
     2101 tgccgcaggc tgtgtccgat gaagatctgc gtcacgccgt tttggtttgc gaaccggagt
     2161 atggcctcga tcgggtcgcc gtcttcgagc acttccaccc gggctgaggc gtctcgggca
     2221 agggcgaggt gggattccag aagctcctga tcggctgccg aaagagaaga ttgctggaca
     2281 taggccacga tcagctcgcc gtgaaaccgc tgagcgttgc gccggccgct ttcgatcatc
     2341 agccgcgcat tcgcccgcgg cgtgatgcac accagaatct tctcgtgagc gccgatcgag
     2401 tggccgattc catgggcttc gagataggat tccaactgcc ggtccaccac ctcggcagcg
     2461 agcagcaacg ccatctcccg cagctcggag agtcgccgct ccgttacgag gcggtcggtt
     2521 tccgagcgca ctcccacgcg ctcgagaatc tgctcgggag gcgcatcgac gatggcgatc
     2581 tcttccgcgt gcatgaggaa ggccttcgga accgtttcgc ggacctcctt gcccgtgatc
     2641 tgctgcgtag tatcgtgcag ttcttcgatg tggttcagat tgacggcggt aatgatggcg
     2701 atgccggctt cgagaagttg ctcgacgtcc tgccagcggt gcgcgtttcc gcagccggag
     2761 gggtttcggt gtgcgagctc gtcgatcagg cagacttgcg gccgccttgc gatgatagcc
     2821 ggaacgtcca tcgcacctcc gcgatccgcg cagatctgcg agatgatttc gaaccggtcg
     2881 agcagactga taaggtccgg cgagagtttc gactgcaccg cgccgatgat tacgtcttcg
     2941 cctcgctcct ttctgcgtct accctcatcg agcatgcgga aggatttgcc gacgccggac
     3001 gcgtagccga gaaagacctt gagcctgccg agcctgcgtc gctgctcgtc gaactcgatc
     3061 tggcgcagca gccggtcggg atcgggccgg ctgtccgggt tcgaagccac tgctgctcta
     3121 ccagcctcct tccttggaac cgatccacca gatcagcccg agtgtggcgg tggcctggtc
     3181 cggtttcagt atgccgggtt cggcggtcaa aaagaagggc atattggacc aatcccggcg
     3241 gatctcggct cgaagctgaa aaccggtgtc aacctgatag gtcgtggtca acgtcgtgtc
     3301 cttcagcaac tggtcggtgc cgctgaacag gccggccctg tccctcagcc agccgaaccg
     3361 catcccgagc gagaacttcg gcgtgaactg gtagcgcgcg tagccggcgc ccccgtagac
     3421 aagagcggga ggcgaaacgg cttcgacgcg gttgaccacg taatcggcct cacccaccag
     3481 ggtcagacgc ggtgtggcgt cccaggccgc ataggaatca agaatgtgca gccggccgcg
     3541 cgggaccggt cgaaacagtg ccgttgagag gccgggctgc gttggcaggg ctggcagccc
     3601 ggggttcaga agcggctcga gatcccgttg ctcgcggccg gcgtaatatc cgatgctcca
     3661 ggtgacggag gacgccggct tccacgaaag caagaggtgc tgcgacttga agccgttgaa
     3721 gtcctcggtc tgattggcgc cgttgacgag ccagtgcgtc agtatgagcc ggtcattcac
     3781 ctgatacgac gaccggaaac cgaagtggta aaatggcaga aagttgaacc agtaggaacg
     3841 cgagtaattg atctggtcct ttgcgtagtt gccctcgaag ccaagcgaac tggcccactt
     3901 tccgaaatcg atcctcaagc cgttaccgag cgggaagaca tacgtgccgt aagcctggaa
     3961 cacgggcctg aacacctgtg gacgcggctc gttctgcgcg ccaccctgca aggtttccgt
     4021 cccctgcccg tacatcagat caagccgaat accgaagcgc ctgccggacc cggtatcggg
     4081 aggccgttcc agcacgagtg tggcctggtt caaactgaag ttgttgctag tgacgtcgta
     4141 ggcgcggagc agattgatcc gaccgagagg gcggctaaag ttgtagctga aatagccatc
     4201 gagatatgcg ttcacgctga tttgcggtgg cccgggtgat ggctcggccg aggcacgttg
     4261 agcgggttgg gacggtattg cggaagcatt cagcttggct tcgacggcgg cgaggcgcgc
     4321 ttccagctgc cgaattcggt taagaagttc agcctcgctg gcagtctggg cgagagcggc
     4381 agatgccacg acaatcatca ccgccagtcg cacggccgtc atttccgcac cgggaaatcc
     4441 tggtcgaggg ccaggttcag ctcgagaacg ttcaccgccg ggtcgccgag cactccgacg
     4501 gccgattcca cggtgaactg ccttgtcaac aacagcactt gctcgacgct gactcctcgt
     4561 gcgcgcgcga cccggggcgc ttgcgcacga gccgccgcag ggctgatgtg cgggtccaga
     4621 ccactgcccg aagcagtcac catgtcggcg ggaaccggtc cgcgatgctc ggggttctgc
     4681 ttctggaact catcgataaa tgctctgaca cgctcgacga gcttgcggct ggtcataccc
     4741 aggttggagc cgcctgacag ggcagcgtcg tacccctcgc cggccgcgga ggggcgcccg
     4801 tggaagtact cgggccgggt gaagggctgg ccgatgagac gcgatcccac cacgcgtccg
     4861 ttccgctcca ccaggcttcc gttggcctgc cgcgggaaca cggcctggca gattgcggtc
     4921 accagcgctg ggtagagaag acctgtgagc aaggtcatca gcaagatcat gcgaactgcc
     4981 gtcatcttga cctcctcacg caagcctcaa gaagtagagc aactggtcga tcagccagat
     5041 cccgggaaat ggcgcgatca ggccgccgag accgtagatg agcagattcc ggcgcagcag
     5101 cgccgccgcg ctcgccggcc ggtacttcac tccgcggagc gccagcggca cgagcgctac
     5161 gatgatcaga gcgttgaaga tcaccgccga gagcacggcg ctttgcgggc tgtgcaagcc
     5221 catgatgtta agcgccgcga gcgacggata tgttcccatg aacatcgccg ggatgatggc
     5281 gaagtacttg gcgacatcgt tggcgatcga aaacgtagtc agcgcgccgc gcgtgatcag
     5341 caactgcttg ccgatcgcga ccacctcgat gagcttggtc ggattgctgt cgaggtccac
     5401 catgttgcca gcctctttgg ccgccatagt gcccgtgttc atcgcgagcc caacatcagc
     5461 ctgcgccaat gccggcgcgt cattggtgcc gtcgcccgtc atggccacga ggcggccggc
     5521 cgcctgttcc ttccggatgt actcgagttt gtccttcggc gtcgcctggg ccaggaaatc
     5581 gtccacgcct gcttcctgcg cgatggccgc ggcggtgagc ggattgtcgc cggtgatcat
     5641 gaccgagcga atgcccatcg cccgaagctg tgcgatgcgt tcccgcaagc cgcccttgac
     5701 gatgtctttc aggtgcacga ggccaagcac ccgcgagcag tcggccacag ccagaggggt
     5761 gccgccagaa cgcgagatac gctcggcggt ctcggtgaag cctgccggga cggcgccccc
     5821 gaactgccgg acgaaggcgc cgatcgcgtc ggtcgcgccc ttgcgaagcg aacgcccgtc
     5881 caggtctacg ccgctcatgc gcgtataggc tgagaacgga aggaaatgcg cctggtgctc
     5941 ggccacatcg cggccgcgca ggcgatactg ctgcttcgcg agcaccacga tggagcggcc
     6001 ttccggcgtc tcgtcggcca ggctcgccag ttgggcggca tcggcgagtt cggccgcagt
     6061 cacgccgtct gcaggggtga actcgacggc ctgccggttt cccatcgtaa tcgttccggt
     6121 cttgtcgagg agcagcgtgt tcacattgcc cgatgcttcg accgctcttc cgctcattgc
     6181 gagcacgttg tgctgcatga cccgatccat gccagcgatg ccaatggcgg agagcagccc
     6241 gccgatcgtg gtcgggatca ggcacaccag cagcgctgtg agcacagcga ctgtcggtgt
     6301 cttgcctgct cccgccgcaa tgacgctgta ggcggcgaac gggtggagcg tggcgaccgc
     6361 cagcaggaag atcagggtca gacccgcgat caggatattc agcgcgatct cgttcggcgt
     6421 cttttgccgc tcggcgccct ccaccagcgc gatcatgcga tcgagaaacg tctcgccagg
     6481 gttcgaggtg atgcggatgc ggatgttgtc ggagaggacc ttggtgccgc ccgtgacagc
     6541 actgcggtcg ccgcccgatt cgcggatcac tggcgcgctc tcgcctgtga tgacggactc
     6601 gtccaccgtc gcaattccct gaatcacctc gccgtccccg gcaatgatgt cgccggcttc
     6661 gcacaggacg acgtctccgg cacgcagacg cgaggcggcc acacgttcga ctcgtccgct
     6721 gtcggaaacg cgcttggcca cggcgtcggt ttttgttttg cgaagcgcgt cggcttgcgc
     6781 cttgccgcgc gcctccgcca tggcctcggc gaagtttgca aacagcacgg tgaaccacaa
     6841 ccagagcgcg atctgaagcg aaaaaccggc cacgccggag ttggaaaccg cgtcacggat
     6901 caattgcgcg gtgacgagcg tagccccggc ctcgaccacg aagatcaccg gattccggat
     6961 cagccgcgca gggtttagct tagcgaagga ttcgcgcgca gcgcgcttca gtatgcgcgt
     7021 gtcccacagc gggcgggaat gcgcaagtct cttgggaaga agcgaaggca ggtccgtatc
     7081 gccggtggtc atggctaaaa gagcctcccg ctatgcatca ggaagtgttc gacgaccggt
     7141 ccgagagcca gggccggaaa gaacgtcagc gcaccaacga tgatgacggt gccggccagc
     7201 agcccaacga acagtgggcc gtgtgtcggc agcgtgccgg ccgagagggg cagccgcttc
     7261 ttgccggcca ggctcccggc caccgcgagc agcgggatga tcatcagaaa ccggccggcg
     7321 agcattgcca ggccgatcgt caggttgtac cacggcgtgt tggcattcaa acccgcgaac
     7381 gcgctgccgt tgttcccggt cccgctcgaa taggcgtaca ggatttcact gaagccatgc
     7441 ggaccggcgt tgttcaggtt tgcggtggcc ggcccaggag gattccagta cccgttcttc
     7501 tcgaaatcca agaccgaaga agcggacgta aggagcagga tactggcggc cgtcgcgatc
     7561 agcgccagca tggccatctt gacttctttc tgttcgatct tcttgcaaag gtactcgggt
     7621 gtccggccga ccatcaggcc cgcgatgaag accgcgagaa tggcgaacag cagcatgccg
     7681 taaagcccgg agccaacgcc gccgaagatc acctcgccgg cctggatatt gaacagcggc
     7741 accagcccgc cgagcggggt gaagctgtcg tgcatgccgt tcaccgcgcc gcaactcgcg
     7801 ccggtcgtga cggtggcgaa gagggccgtg gcggcgatgc cgaaccggac ctctttgcct
     7861 tccatgtttc cggctcgtgc atcagcgccg agtgcggcca gcatcgggtt gccggatgcc
     7921 tcggcgcggt aagcgacgaa agccccggcc aggaagagca cgctcattgc ggcgaacagc
     7981 gcccagccct gccgcgtgtc acccgccatc cgtccaaacg tgtacgtgag cccgccggga
     8041 atcaggaata tgaggagcat ctcgacgaag tttgtcagcg gggtcgggtt ttcgtaaggg
     8101 tgcgccgagt tggcgttgaa gaatccgccg ccgttcgtgc caagttcttt aatcgcttcc
     8161 tgcgatgcca cgggtccctg cgcgatcgtc tgccgtggcc cctcgatcgt ctccgcgacg
     8221 acgtagggct tgaaattctg gatcaccccc tgcgaacaga gaaacagcgc ggcgacgatc
     8281 gacaacggca gcaggacgta cacggttgcc cgggtcagat ccacccagaa gttgccaatg
     8341 tgctcgctcc gctctctcgc gaaccctcgg accagcgcga cggcgaccgc catgccggcg
     8401 gcggccgaca cgaagttctg gacggcgagg cctgccatct gtacgaaata gctcagcgtc
     8461 gtttccccgc cataggcttg ccagttcgta ttggtcatga agctgacggc ggtgttaaac
     8521 gccaggtcgg gagcgacctg cttttcaccg aatcccatcg ggttgagcgg cagcactccc
     8581 tgtagccgct gaagcgcata cagcaggacg aaactcgcgg cactgaacgc aagcagcgat
     8641 ccggcatatt gcgtccacct ctgctcggca cgttcgccga cgcccgagag ccggtagatg
     8701 agcccttcca agggccgcag caccggatgg aggaacgtcc gctggccctg aaagacctgc
     8761 gccatgtacg ttcccatcgg ccttaccaca agaatcagga tcaggaaata gagggcgatc
     8821 tgaacgactc cggcctggct catctcaatc tccttaaaag cgctccggct tcagcagggc
     8881 gtagagcaga taggcgaaca gaagaaccgt ggcgagaccc gcgaccaggt agtccatgtg
     8941 cctcctctct acagccgctc acacgcccgg acgagggccc aacaagccgc gaagaacaga
     9001 agtgttacca aaacgtacgc gaaatccagc atcagcttcc ccattcgtgc gtgcaaaata
     9061 cgacgtcgtg ccccagatgg gccagctcgg ccgcaagccg ttgctctcga gtcggccacc
     9121 agcttcgccg gccggcgatc acaacgagcg attgcggccc cagagctacc gccagcacgg
     9181 tgcgccggtc ccgacagatg tagactcgaa cgtgcagatc gatcgccatg tcgtccgcaa
     9241 ccgtccggaa acggtggacc aggaatgcgg ttgagattgg cggcgcttca atcggcgcag
     9301 gataaggcac gacctgcggc actaccacga caatgtgcgc gcgaagatca gccgcgagtc
     9361 gggcagccgc tcgcaatgct agcagcgtct ccgcaagatc ggtgaacaca acgttcaccc
     9421 gcagttcccg atcgtgtcgc tccgcggctt gcggctgctc ccccctgcgt ttgacgcctg
     9481 cgttcacgcg cgcctaccca ttccgagaat aggagccccg ccgtaaagaa gggataaagg
     9541 aaggctaagg attctctaaa tctgagcggg agggcatcac gaaccgatag ccgagccagg
     9601 gctccgtcag gatatagcgc ggcttcgagg gttccggctc gatcttcttc cgcagttggt
     9661 tgatgaagac gcgcaggtac tcgacttcct ggccgtaatc cggtccccac acggcctgga
     9721 gcagcttgcc gtgcgggatg ggcacgttcg ggttcgtcac caggtaatga aggagatcga
     9781 actctttggg cgtcagtcgg acctctttgc cttccttcgc tacgcggcgc gttccgaggt
     9841 tgatctccaa cccggcgagc tcgatcacac gatgcacggg atcaccggca agcggcacgc
     9901 gtctgagagc ggcacgaatc ctcgccagca actccggggt tccaaacggc ttcgtaacgt
     9961 agtcgtccgc gccggcgtcg agcgcgtcca ccttgtcctt ctccgagctg cggaccgaaa
    10021 gcactacgat ggctgcatcc gaggtgtcgc gaatcaaccg acatgttgcc agaccgccga
    10081 ttcccggcat gttgaggtcg agcaggacga ggtcaaattt tgccgcgcgc aacttctcca
    10141 gagcctcttc gcccgttcgt gcatcatcta cctggtagcc ttgggcaacg agcgttgtgc
    10201 gcatcacgcg gcggatctgc ggctcgtcat cgacaacgag gatgcgcgcg gacgtcattc
    10261 ggtcactccg tcttcaatga tcggcagtga gaatgagact cgcgcaccgg gtcccggcga
    10321 gtcttccacc cagatccggc ccccgtgcag cgtgacgatc tccctggcaa tggcgagtcc
    10381 catccccgtg ccggaggatt gcgttctcgc ccatgggcct cggtagaacc gctgaaaaac
    10441 gcgctcacga tcctcgggtg cgattcccgg acctgtatcc gagacggtaa ccatcaccgc
    10501 gccgccgtcc gcgcgcgcgg tgatcgagac cggagatccg gcgggcgagt acttgagagc
    10561 gttttcgagc agatgtccga taacgacagg gatcaactcg cgatcggccg cgacgagcgg
    10621 cagatccggc ggcccgtgca cggagatctc ccgttcttcc ctgccaggca gcctgcgcac
    10681 gatccggtcg atcagttccg cgatctgcgt aggctgtttc tccaccttta cgtccccggc
    10741 ttctactcgc gccacgcgga tagcgtccgt caccatggag ttcaaccggt ccgtttcctc
    10801 gtcgatcacg gtgagcagct cgacctgcgg ttccttcagt tcggcatccg taaggagacc
    10861 ggtggctgct gctttgattg ccgtcagcgg agtcttgaac tcgtgtgcca gagcgtcgag
    10921 cagcgtcgat ttcaattgct cgctctgccg tgccgcctcg gcctggctcg cggcttccag
    10981 actccgagcg cgctcgagtg cgatcgcggc gagatttgca acagcattca gcgcggtgtc
    11041 gctgatctcg gtgacggaga tcgccatcgc gcctaagggc tcaccgccta gccgcatggg
    11101 gacgagcgat gtgtttgtgg ccgcgtcgtg aaacggcgtg tcctgcaaag cgacgtcttg
    11161 caggcggctc tcagtgaccg gaacatcttg tgggccagca cgatggaatg accgccggct
    11221 cttgtcgtag atcatcacgg ccgggctttc aaacacctgt acaatgagcc cggcgacgcg
    11281 cttggccgct tccggcccgg tttcggccag catcatggcc cggctgaggg tgtacaggcg
    11341 ctccatctcc gcccggcgct gaatcgcctc agtcgcgcgt ttccggatgc cggcggaaag
    11401 ctggctggcg acgatcgcgg tgacgagaaa agcggccagc gcaacccagt tctgcgggtc
    11461 ctcgattgtc agggttccca tcggcgggag aaagaagtag ttgaaacaga acattgcggc
    11521 gaatgaagcc gtcactgcct gtcccagccc ccacgtcgtg gcaaccgcga gtacggccag
    11581 cagaaacgtc agcgcgacag tggttgcgtt gacgggaaga aagaggaagt acacggcagc
    11641 gagcagtccg atgatcgcca atgcgccagc gtaggaggcc atcctgaccc ctttgcggtg
    11701 ggtgtcgttc gctgccatgt gtgaggcacc atcttccttt ctgattgtag gcttgctgcg
    11761 gctcagccga tctcataatc gcttagttga ggcgacgctg tgctactggg aattgcacgt
    11821 aggccgtcag aggctgtcca gaagggtgct cggagctgcg gtccacccgg acagacatac
    11881 gtacgacagg gcgtgcgcgc acgggccggg ggatcccgcc gccgccctga tgccgatgac
    11941 gttgtcagga ttgccttaac ggacgcgacg aaatcgactc tctcgtttca cggttgcccg
    12001 tcccgcctgg aggtacatcc gcctgagcgg aaggctgtgc ggcagctctc ttcttggcct
    12061 gcgttaccag atacgcgtac cgaatgccgt gaataatccc gaacaggatg aggagcaggg
    12121 tcgcaagcgg aatcagaatc gtgtaatcgc gcatcatgtg gagctctctg cgaagggcac
    12181 tccctctcca tcatggacgg cagaccataa gaaactcgta aagaagtcgt caggatttct
    12241 ttatgtttgc acgaactaac gtcgccgccc cagtggtttc tgattcggtt gcttccgttc
    12301 tggacgaacc tctcttcgac tctgccgtca tccggctgct ttagcgagcg cgccctatgt
    12361 cgtgtcctcc accgaggcgc tccgcatcct gtcgaaggcc agccgtgaat tgatgcgtaa
    12421 cggtctgccc ggactggacg aggtagctga aaaacgcgcc ggttggaccg gacggaggct
    12481 aatggcagca aaattaccgg gaaagcgcgc agtgagcccc gtcccagcgg tctatgaagg
    12541 agacgatccg gcctgcagtg caatgctcaa gctgggtcag tcagattgag ggggatcggt
    12601 gcacgccgaa tcgtctcggg gattaagaca aggctatcct tttttcgcgt caaggggatt
    12661 ctggggccat acggcgatgg ctggtggggc aatcgacata ggtaggctgt cgccagcttt
    12721 ccctgtcaca ccaccgtacg tacgggtccg tatacggcgg ttcggcgggt tgagctatcg
    12781 gccagcagtc aatctcggaa tcccgagcga gtcgaagtag gcattgggca gcgcaaaggc
    12841 gagtgccggg ctgttcgcca gcctccacgg accgtgagcg ctgcccgccg tttgcgcggc
    12901 gaggtccttg cccacattcc gtttccgcag ttcggtaaac cgcaccgatc cccgcgtcca
    12961 ctgtttccag atcgcagacc ggagcctgcg ccggaaccac ttctcaaggt cttccaacac
    13021 cgagggcgtt tggcacctgc cgaaatagcc gatccagccg cgaaggtatc gggccagttc
    13081 ctcagccatc ctctcgatgc tcacgcctcg cgtccggctc gtcagttccc ggagcttcac
    13141 tttgaaccgg agcaccgctt tgggcgcgat ccgccgtttt ggttcccgat tcgccgtgaa
    13201 actgaagccc agaaactttc gctcccatgg ccgtgccacc gcactcttct gctggttcac
    13261 cttgagcttg agtttcgtcg tgatgaaccg tgtaatgctc gccatcactc gctcccctgc
    13321 ccgccggctg cgaacataga tgttgctatc gtccgcgtac cgcgcgaacc ggagacctct
    13381 ccgctccaac tcccggtcga actcgtcgag cacgatatta ctgagcaagg gcgacaacgg
    13441 tccgccttgc ggcgtcccct catccaccgg actcaccagc ccgctttcca tcaccccagc
    13501 cgtgagaaac gcccgaatca gcttcagcat tcgcttgtcg ctgacccgtt cggcgatctt
    13561 ggccatcagc ttgtcatgcg aaacccgatc aaagaacttc tctaaatcga ggtccacgca
    13621 ccagcgatat cccgcggcga tatattgctg cgcctgttcc accgcctgat gggctgaccg
    13681 tccagggcga aacccgtagc tgtggtcgga gaacgtccgg tcccacctgc gttgcagaac
    13741 ctgcatcacc gcttgctgga tgaatcgatc cagcaccgtg gggatgccaa gctttcgcac
    13801 ccctccgtcc ggcttcggga tttccacccg ctttaccggc tgcggcacat aggccccgct
    13861 caacagctgt tcccggatgg ctggccactg ctgtttcagg tatcccacaa gttcgtgaac
    13921 tgtcattccg tccacgccgg gctccccttg ttggccttga ttcgtgccag tgcctgtttg
    13981 cagttttccc gctcgcagac ttcttccatc aactgttcgc caatggccgg gctttctgcc
    14041 ttgcgcttcg ccgcgagcga ttcggtccct tcctcggcag cgcttggggc ttcacccgta
    14101 tcctctgcca ggaaggccag cactaggtct atgaccgttt gaaagatttt cttttatagc
    14161 cgaatttgcg acgggcggcg cggcggtcga atttccagtt gatcttgatg cggtcgcggt
    14221 tcatgcggcg gttccaggcc cggctttcgc ggcgcagcgt ctgaagatca gggattcttc
    14281 tggtaccgag gcattgccgc gagaagatgc cgatttcgat ttccgcctga ttcagccagc
    14341 ttccgtgggt gggtgtgaag tgaaccatga actggtccca gatctcgctg ccgacctcca
    14401 ccccgaagac gtccgtcaac gacttgcagc agtggatgtt gaggttgtcc atgaccaggt
    14461 ggatggtctc cgcatccgga tactgcatcg ccagacggca ggcgacttgg gcgaactcaa
    14521 aggctgatcg atcgggcgtg ggaaaggtga aatgccggcc cgccttcggc tctacggcac
    14581 agaaaacatt ggctgtgcca cgccgctggt actcgttatc ccgtcgcgct tcccgtcccg
    14641 gctcggccgg agaggcgggg cggacgtcag cgtgtagcgt gaccggcttc tcatccagac
    14701 acaccaccgg cgccttggga tcgtacggtt tttcatacgt ctccagcacg tcctccatct
    14761 tggcgatgta ctcgtcgttg agttcggcca cgcaccacat ttttttcccg ccacggcttg
    14821 aggtcgtggt cgaggagcag aatccgaatc gtttcccttc ccacgcgtgg aaccagccgc
    14881 cgttttacgg cttcctgcgc aaccagtcgc acggtccagc gggcgcggcc ttcgggcggg
    14941 tcgctgcaca ccatggcgat gatccgctgc ttctggctgt gttccagcac agccgccgcg
    15001 ccggggcgcg gcttgtcata gagagcgggt tcaagacctc cctgttggta acgatggccg
    15061 accttccgaa tggcctgcgg agtgagtggg accacgctgg aaatccgcgg cgcactgaca
    15121 cccttggcca actgcaggag ggccagggct cgcaggacga cacggacctg ctgtaggccg
    15181 ccgctcaaca gcttcgtcag ttcgtcttgg tccctcggtt tgactttgat actcagggat
    15241 gggcgtccca taccccaagt atgcgccaaa acatttcgaa ttggaattgt tttttagagc
    15301 tttttcatgc ggtcaaagac ctagctcctt ttctacctag gagccagcgt caccagcgcc
    15361 agcgtgtatg tctgcaagcg cggcacggtg aaggtgatgc ggtcttcctt cgccacgaaa
    15421 ttcacttgac ggtcttcgcc cccatcgggc gacatcagcg tcaccttgga cgccttcttc
    15481 ccttgaggga tacgcacact cacctcgata ttgctcaccg acggtgtttt caccctgccg
    15541 tagttgacaa ggtgaatcag taattctccg gccggtttct gctccatcag ttccgccacc
    15601 acgtttggcg gcgccttgac ctcgagtgac atacgggccc ccgccgccca ctgcacggca
    15661 tcgatcaggt ccttcgcgtt ctccggcagc ttccagtact ggctggtcat acgggctcct
    15721 ggtggcttcg gtatcgaggg tttgacatcc gaaatgtaga cgacccgccc cttcccggcc
    15781 tggttccgaa ccggaggcgt ctcctgcact gccacctggc ctctcctgac cagcctgggg
    15841 gattcagcgt ggaaaagatc ctgcaagccg aatgcgggcc tccgcaggcg ccactccgtg
    15901 aacaacgtcg agagctccgt ggccaccagg cctccaccct gctcgacgaa cttccggatc
    15961 acgtccagct tttcgtcatt caggcactcc tggtcggcca ggacaaggac acggtacttc
    16021 gacagatcct tcagatggtc gtcgaagatg aggtcgaatg gaatcctggc ctgaataagc
    16081 gcctgctcga acaacatcgt gctgacggcg gggcggtcgt tgttgtaccc catgctggcg
    16141 taagagtgga gcaccgcaac gtcggcgatg ttgtccacgt ccctgtaatg atcgaagttt
    16201 cggcggtaga agttgatgta gtcccgctga tcttgcggca ggtcgtagcc tgccagggtg
    16261 ccgccaacgt cgcccaggca ctgccggtta aacgccatcg attcggcaag ctgaagcgtg
    16321 cttccacccc cacccccgtt ggcaacgaac agcgtgttct tcagaatgct agccatcttg
    16381 aacgttctaa tcttgctgac aaggatcccg tcctcggtga cacctgccgg atcgccctct
    16441 tccgtccaca cggcgtccat gtgggcaagc aggcgcgggt aatccacgcc ctgctcccag
    16501 atcgtgttgc gccccgaaat gcccgagtgc gggttggtct ctaccgcaac ctccgggtta
    16561 agtgtgcgaa gcagttcttc gagttccgcg tagtaagccg tcaactggac gcaacgaaag
    16621 tcggtccatt cctggaagag cggatcgttg atcgccgtca gcgggcggtc atacctcggg
    16681 ggaagcatgt accggacctc cgaaaacccg aacctctgct tcaactgttc cggcgagtac
    16741 ttggtctgga ggaacttccg aaagtcatcg atcgccatcg gatgctgaaa aatgggaggc
    16801 tcggcctgca tacttgtgtt gtcgaaatgg atcaggtctg ccttgaactc cttttccgcg
    16861 atggtgagga cccgtttcat gtactcgcgg tattcggggt gcatgaaata gacccgttta
    16921 cgaaaggttt ggtcgtcata gaagacaggc cgcccgagga aatcgggcac aaaccacttc
    16981 tcggcctgcg gcttttcggc caggaatgtc tcgtaggcga tggtgctgcc cacgtacacc
    17041 cccacgcgca tcccgtactt atgcacgagg gcagcggtct gcttggcctt ctccatgtgg
    17101 tccttctcgg cctcaagccc aaagcccttg tagaagtgga gaatcaccag ggtcaccccg
    17161 atgtccttca gcttgcggat ggcttcctcg gagtctccct ttatgtattc ttcctcctgc
    17221 cccaccgggc tcccgccacg ccggcgctgg aagatcggca tgctgtcgtg gttcgacacc
    17281 atgaccagag gggtggtgtg gagccaggaa ggccgggagg cgcttacttg tttcttcggc
    17341 gcgaccggtt ccggccgcgt catacacgat gcgagagtga acactacggc gagcaacaga
    17401 gcaacacacc caaaggtgca aatcagacgc cgttccataa tcatcacccc agattgatta
    17461 ccacgagact gtacgtactt agcctcggca cagtgaactc cgcgcgctgg cctttcagcc
    17521 tgggcgcaat ggtcaaagac ttgtcttcat ccggtgagcg cagcgagatt cgctctacct
    17581 ttttcccgtc cgggatctgt acgctgacca caacgccgtt gacgacggga tgccggagcg
    17641 catcgtagtt gatcagatga acaaccatcg cgccggtctt ctcctggcgc agcagttcgg
    17701 cggtgacggt gggcggcgcc ttcacgtcga gagagaggtt gccgcccgcg gcccacctga
    17761 cggcggcgat caactcctcc gcattgaccg gaagcttcca atactcgctg gtcatcgata
    17821 cggagggagg cttcagcatg gccggcttca cttccgggat atagaccacg cgaccgttgc
    17881 ctgcctgccc cttgaccgcg gggcccttca tgatcgcatc cggcgtagcc ccttcttcca
    17941 atggcggcgc gctaactctg aacagttcct tgagcccgaa gtcgggccgc cttcgccgcc
    18001 attccgtgta aagagaacta tgctcggtcg ccacaagacc gccgccccgg ctcacgaact
    18061 tgcggatcag atctagttcc gcgtcgctga gacactcctg gtccggcagc accaggacgc
    18121 gatatttgcc aagatcgttt agttgctggt cgaagatgat atcgaagggc gctttcgcct
    18181 gaatgagggc ctgctcaaag agcatggcgc tctgccaggg ccggtcgttg ttgaacgcca
    18241 tgctggcgtg agactgaagc acggcgacgt ccgcgacatt gtctacatcg cgaaaatgct
    18301 ggaagttgtc aatgaaaaac ctgatgtact tacgctgatc ctcgggcaac tggtaaccgg
    18361 cgagcgctcc acccaccatc cccaggctct ggcggttgta ggccatggac tcagccatct
    18421 gaatcttgcc gccgcgtgtc cccgcggtgt aagtgatgac tcgtttcttc aagctggtgg
    18481 ccatcttgta ggttcggatc ttggagacca ggatgccgtc cggagtgacg gtggcctcat
    18541 tgccctcctc cgtccagacg atgtccatgt ggggcaggaa ggtcgggtag tcgacaccct
    18601 gctcccagac cgtgtttcgc ccggagattc cggaatgagg gttggtggcg atggctgcgt
    18661 tcggattcat gtggcgaatg tgccgggcca gctccgcata gtagctgctg agctggtagc
    18721 agcggaaatc agcccattcc tgaaacagag gatcgttgat ggctcgcaac ggccggtcat
    18781 agcgcggcgg caggacgtac cgcatgttcg agaagccgaa ccgtttgacg agggcttcct
    18841 gcgggtatcg gctttggagc cagttgcgaa aatcctgcac tgccatcggg tgttcgaaga
    18901 tgggcggctg cgcctgcatg ctggtattgt cgaagtcgat ctcgtcgacc ttgtagtctt
    18961 ccacggccag ccgcaagaca cgtttcatgt attcgcggta ccccggatgc atgaaataga
    19021 ctctcttgcg aaatgtctgg tcgtcataga agaccggcct gcccagaaag tctggaacga
    19081 accagtcttc tgcttttggg ttctccgcga ggaatgtctc gtaggcgaca gtgctgccaa
    19141 tatacactcc ggcacggacg ccgtacttct tgagcaaagc tatgtacttc cgtgtgtctt
    19201 cgagatgctc tttttcggcc tgcaagccga aacccttgta gaaatgggtg atcgcgagat
    19261 tcactcccag atctttgagc ctcttgacgg catcctcggt catctccttg cgatattctt
    19321 cttcctgaga ggttgagctg ccgcctacgc gccggcgaaa gataggcatg ctgtcccagt
    19381 tgcctaccac aaccagcggt tcgtcattca cccagccggg ggtcatgagg gacgccgatt
    19441 tttcgggcgc gacggcctca gtcttcggcc ggggtcggga acacccgcaa acaaagagcc
    19501 ccaactcaag cagaaccgta agcgaacaac agctcttaca cataagccag gatttcctcc
    19561 tacaccggcc ttccagccga cattaagcta ccacaatcgg cctgtcttgg agccgttgaa
    19621 tctccaaggt ctccatcagc cccgcatgcg gaaagggccg gtgagacaaa ggtccccggc
    19681 ggcgttgcca cgcgagtcaa gggctccctg gtcacgttcc caggctgaac gccggcagga
    19741 tcgagtcctc tccgctgcga ctcgattctg agaggttgca caacgctgac cgggatcgac
    19801 gcagcgccct tcctcgatca cggcctactc agaaacaggc gcgccaaaaa cactccaccg
    19861 gctaggcctc gatggacgtt gaatagaaaa agatcctagc cgctctggac cgtgcgagaa
    19921 ccggaacctc ttgccgcagg gtcggcaggc ttgggaaatc cttttcactg tggctctggc
    19981 taccgactgg gcctcatcct gaagaagatc ccatgggttc actcgcagaa ctatctggca
    20041 cagggggatg acgttcaacc ccagcgctgg acggctgggg ccagcccggg cgccgcagtc
    20101 atggtgtaaa cggcactcat tcccgccgtg gctgtctggc cgacgttgct gagagggtga
    20161 agttcgtgaa tcgtccgtgg ccgtgaaact cgtgaacccg aaagccgaat aggtgctgcc
    20221 gcatccgtaa ttaaatccca cgcccggact acccgtggcg aaagtagtat ccgtcgccgt
    20281 cgcaatcagg acatcgttga tgtaagcatt gatcatacta ccgacaatag ttgcctttac
    20341 cacgtcgccg ttggccactc cgatcccgct cacgcccccg cacaccgcag gatatgaaca
    20401 acgtcctccc aggtaggtaa aactggccag cgggccgttc cagcgtacaa tctgcacgta
    20461 agccatggag ttgttcgggg ttcgaaacaa gatctcatag ccggtggtgc tgtgcgccga
    20521 aatggaagtc cgaagccgaa gttctacctc ttggtagtag ctgttcgatg cacccacgct
    20581 gtaaaccgtt gctgttactg tctgagtcgg cctccaattc ccagccaaaa tcgccgtcgg
    20641 atcagagtat ttcactgacg acggtccgac gccgccgacg aatcctggag ttgtctgcac
    20701 gttgttccaa tctagcccaa ccgtgccccc attgatccac atgccgccct cagaaatggg
    20761 attctccgta cgggggaagt tggtggtgta ggttcgcgca gaggcagaaa gggaaagcag
    20821 accagtggtc agaatgaacc ggaagacatt gcgcatgggc ggatttctcc tccggctgca
    20881 cagcacctca tggatcaggt ccatgctctg ttttacccag gatttcccca ctggctgcct
    20941 ggccacggtc gattatatac ccagcggcct ccgtccccga tgccagcgct cattctgcgg
    21001 gtcattacgg cctggaaagc cgctccataa ctcggattag tacgatctcg gtgcggtcga
    21061 ggaggacctg tggttcggca gcttcccccc gattcgcccc aagaaccctc aacggtcagg
    21121 ggaggcacgg tcgccgaacg tccg
//
LOCUS       DATIFQ010000248         7632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_655996, whole genome shotgun sequence.
ACCESSION   DATIFQ010000248 DATIFQ010000000
VERSION     DATIFQ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655996"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /locus_tag="VLE22_11520"
     CDS             complement(<1..1502)
                     /locus_tag="VLE22_11520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSB15080.1"
                     /translation="MKRTSAIFALLVLVLIAGTRDRKGPAPRFDSWRIIGPGGAGGMF
                     LPTVSPHDPNIVLEHCDMTGTYISLDGGESWRMFNLRSTVSALAFDQKTLGVLYAGNA
                     VLWRSEDTGKTWSMVFPDPKKNTVERMRDDHAAPLFTTDDPAFPSRHQVRFIAVDPND
                     SNRIYVTFAGRGQVTRTVAPPTTALFESRDYGKSWAKIKDFPAEQVHLVYMLPGSPDL
                     RVVGESGVYRLSDRQWRHYPAPPGVKFISASAGAQNGVELIYTTTGKRGGVYVSRDGG
                     RTWENPRTALPGQPLFLSIACSSRNAGTAYVGFDTQRTSGDDAEHYGVARTADGGKTW
                     SICYRESSEPADNVEHSWVADFYGGAGPIRDIFVAPTNPNIVHLTDSCPRALRTTDGG
                     RTWEEEMAAFRGKDRWGRDKWATIGFDVTTCYGVHFDPFQPKNLLISYTDIGLWKSID
                     GGQSWMSSIFGVPPHWFNTTYWAEFDPKVPGLVWGAFSRTHDLPRPKMWR"
     gene            1572..1856
                     /locus_tag="VLE22_11525"
     CDS             1572..1856
                     /locus_tag="VLE22_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15081.1"
                     /translation="MKTEVYSWRLSSEAKMALEDEARRAGMTLSALLDQVTEEWLEAR
                     RREAPGDVEAQARLHAAAGRCIGMIAGGRPRRAETARVAIRKRLARRHVR"
     gene            1846..2271
                     /locus_tag="VLE22_11530"
     CDS             1846..2271
                     /locus_tag="VLE22_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSB15082.1"
                     /translation="MSANVLADTGAILALLDRDDRWHRSCVEAFENLRLPLLTSEAVL
                     TELFHLVGDSSREVDAAWKFVRSGALLLGGIEDGELPALEALMSRYSDRPMDFADATL
                     VHLAGRESISTIFTVDRVDFETYRIEGRRRFRIVPEREG"
     gene            2408..3343
                     /locus_tag="VLE22_11535"
     CDS             2408..3343
                     /locus_tag="VLE22_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15083.1"
                     /translation="MRTWLCLSVLAAMPAMAADLRLGIVGTDTSHVIAFTKVLNDPST
                     PGHVPGARVVAAYKGGTDLPQSATRVDKFAAELQSKWNVEFVPDIPTLCSKVDAILLE
                     SVDGRIHLKEAKQIIAARKPMFIDKPLAATLEDAREIARLAAEAGVPWFSSSSSRFGG
                     IAKAMKHADATGVLTWGPGPLDSNLPLDLAWYAIHPLELLYSLMGPGCEEVTRISGST
                     EEVLIGRWKDGRIGTVRALRAAGSSGAVVYRPKQVVQSDPSMEGGYEGLLEEVVKFFQ
                     TKQPPVSNAETIEIFAFMDAAKRSKEEGGKPVKLR"
     gene            complement(3416..5560)
                     /locus_tag="VLE22_11540"
     CDS             complement(3416..5560)
                     /locus_tag="VLE22_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HSB15084.1"
                     /translation="MRVLPVLLLVFFPGLCPAQKKPVTVDAVVAAREAAEQIPEIVWA
                     PAGDRFAVKQGDELSLFDTSSKNTRRLLSLSDLEAPTAAAAAERWKNRHVKEQTFQWF
                     PDGRSLLVLAGGDLFICPIDGGNRRQLTKTSEAEHEPRLSPDGRRVSLRKGNELHVLD
                     IASRRVIRLTHDATETRWNARLDWVYPEELDLSTAHWWSPDSRFIAYLQFDVTRQPLY
                     PHADLLPLRPVFEPQRYPKAGDPNADVRLGIVPAVGGHTRWIALGGSRDDLLARVYWT
                     PDSKSVFVERLNRIQNRLELVRADIANGPSRVILREEDSHWVDLAGDLRFLRNGQEFL
                     RTSERDGFRHIYIHSEDGHDVRRLTQGDWEVTEIAGVDESSGRVYFVSTSPTPLERHL
                     YSVPLSGGETRRITWTAGTHAILMAPGCKNYLDTFSSFQSPPRRSVFRMDGVEQAVFL
                     EADRTPIEEYDLQPEQIVDVRTSDGASLHARLIRPVGFRPDRKYPVVVIVYGGPHAQD
                     VVNEWMGPSTEQLLASRGFVVWQLDNRGSAGRGHRWETALFRNLGARELEDQKEGIER
                     LVSMGFVDPTRLGIHGWSYGGYMTLYSLLYAPELFRAGVAGAPVTDWRNYDTIYTERY
                     MGLPADNPDGYRRSSPLHFAAQLKAKLLLVHNVEDDNVLYQNTLQMADALQKAGKPFE
                     LMHYPLKTHGLSGLYRRHFLETLTSFFERTLK"
     gene            complement(5638..6777)
                     /locus_tag="VLE22_11545"
     CDS             complement(5638..6777)
                     /locus_tag="VLE22_11545"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15085.1"
                     /translation="MNELSRRGFLAGATTATLSRSANAQADAEPVNVGVIGNGIRGTF
                     LVESLLKLPAVKVKALCDIKADRLDRVATLAARDNPATFSDYRALLERKDIQAVWIAV
                     PCDLHVEMAIAALRAGKHIYLEKPVGITPESVRELLKAAKGSKKVFQTGQVMRSYPYL
                     KTAVERLREGIAGEVIMLRAWRHSSDDMDHNSSSADWFFNAKRSGDLIVENAIHNLDV
                     CNWLVDSRPERAAGFGGTLLWKNDPPGRTNMDGYTLSYEYKNGVKMSFTQLAFHPTGL
                     PHGGWGFQVYGTKGAVDVVYGREGKVSYYKRERGAQPIVLSQSADSAPQDLHFEAFFE
                     AVRTGKTDVPGGLEVGVVAALTSIMGREAIYRKKVMTWSEMGVEI"
     gene            6928..>7632
                     /locus_tag="VLE22_11550"
     CDS             6928..>7632
                     /locus_tag="VLE22_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB15086.1"
                     /translation="MHRFVSRTFLFLAPLLVSADTIAIKAGRLIDPETGTAAPNQVIL
                     VEAGRIKSITADAAAAKGFEVIDLSGYSVLPGLVEAHNHLALTYKMEPEHLQFYLTYV
                     MDSTAIRAIQAIANGVTMLSAGFTVVRDMGNAGLYADTAMRVAIEQGWVPGPTVINSG
                     IIIGGFGGQFNPTPERAELVYPEYLDADTPDEIVKAIRRNILFGAKVIKIMVDSKTYG
                     YTADEIRLFVKEAAKSG"
BASE COUNT         1437 a         2206 c         2556 g         1433 t
ORIGIN      
        1 ctccgccaca tcttcggcct tggcaaatcg tgcgtacggg agaacgcgcc ccagacgagc
       61 ccggggactt tcggatcgaa ctccgcccaa taagtcgtgt tgaaccagtg cggcgggacg
      121 ccaaagatgg aactcatcca ggactggccg ccgtcaatgc tcttccacag gccgatatcg
      181 gtgtagctga tcagaaggtt cttcggctgg aaggggtcga aatggacgcc gtagcacgtg
      241 gtcacgtcga agccgatggt ggcccactta tcgcgccccc agcggtcttt accgcggaaa
      301 gcggccatct cttcctccca ggtgcggcca ccgtcggtcg tacggagcgc gcgcgggcag
      361 gagtccgtga ggtgaacgat gttcgggttg gtgggcgcga cgaaaatgtc acggatagga
      421 cctgcgccac cgtaaaagtc ggcgacccag gagtgctcga cgttgtctgc cggctcgctc
      481 gattccctgt agcagatcga ccaggttttg ccgccatcgg ccgtcctggc gacgccgtaa
      541 tgttccgcgt cgtcgcccga agtccgctgc gtgtcaaagc cgacgtacgc cgtgccggcg
      601 ttccgggagg agcaggcaat ggacaggaag agcggctggc ccggcagcgc ggtgcgagga
      661 ttctcccagg tgcggccccc gtcccgggaa acgtagacgc cgccgcgctt tccggtggtc
      721 gtatagatca attcgacgcc attctgtgcg ccggcgctgg ccgagatgaa ctttactccc
      781 ggcggggcgg ggtagtgccg ccactgcctg tcactcaggc gatacacacc ggactcgccg
      841 acgacgcgca gatcgggcga cccgggcagc atgtagacca ggtgcacctg ttcggcggga
      901 aagtccttga tctttgccca actcttgccg tagtcgcgcg attcgaacaa ggcggtcgtt
      961 ggaggcgcga cggttcgggt gacctgcccg cgtccggcga aggtgacata gatacggttc
     1021 gaatcgttcg gatcgacggc gatgaagcgg acttgatgcc gcgacggaaa cgccggatcg
     1081 tcggtggtga acagcggcgc cgcgtggtcg tcgcgcatgc gttcgaccgt gttcttcttc
     1141 gggtcgggga agaccatgct ccaggtcttg cccgtgtcct cgctccgcca gagaacggca
     1201 ttgcctgcgt agagcacgcc gagtgttttc tgatcgaagg ccagggcgga cacggtgctg
     1261 cgcaagttga acatacgcca cgactcgccg ccatcgaggc tgatgtaggt gcccgtcatg
     1321 tcgcagtgct cgagcacgat gttggggtcg tgcgggctga cggtgggaag aaacatgcct
     1381 ccggcgccgc cggggccgat gatccgccac gaatcgaagc ggggcgcagg acccttgcgg
     1441 tcgcgggtgc cggcgatcaa gacgagtacg agtaaagcga agattgcgga tgtacgtttc
     1501 ataacaagtg tagaaggtcc cattctaaac ggaatggttt ttgcggcgga gcatgtacta
     1561 caatgtagtg catgaagacc gaggtctata gctggcggct ctccagcgag gccaagatgg
     1621 ctctggagga cgaagcccgc cgggccggca tgacgctgtc ggcgctgctc gatcaggtga
     1681 cggaagagtg gctggaggcg cgccggcgag aagctccggg agatgtggag gcgcaggccc
     1741 gcctgcacgc ggccgcgggc cgatgcatcg gcatgatcgc cggcggccgg ccgcgacgag
     1801 ccgagacagc gcgtgttgcg atccggaaaa ggctggcccg gcgccatgtc cgctaacgta
     1861 cttgcggaca caggggcgat tctagcgctg ctcgaccgcg acgaccgctg gcaccgctcc
     1921 tgtgttgagg cctttgagaa cctccggctg ccacttttga cgtccgaagc ggttctgacc
     1981 gaattgtttc atttggtcgg tgacagctcg cgcgaagtcg acgccgcgtg gaaattcgtt
     2041 cgttccggtg cgctgctgct cggcggaatt gaggatggcg aactacccgc gctcgaagcg
     2101 ctgatgtccc gatactcgga ccggccaatg gatttcgccg acgccacact ggtgcacctt
     2161 gccggacgtg aatcgatcag tacgattttc accgtcgatc gcgtggactt cgagacatac
     2221 aggatcgaag ggcggcgccg gttccggatc gtgcccgaga gggagggata gtaaggccaa
     2281 agaggagtgg gcaaggagtc tcccgcaaag tgcgcgaagt tcgcgaagga aacgggggag
     2341 atgccgctgc cagaagcgtg gccccttcct tttggtggcg gctcttttcc ggatatagta
     2401 agtgtccatg agaacatggc tctgtttgtc cgtgctggcg gcgatgccgg cgatggcggc
     2461 cgacctgcgg ctcggcatcg tggggaccga tacgtcgcac gtgatcgcct tcacgaaagt
     2521 cctgaacgat ccttccacgc cggggcacgt gcccggagcg cgggtggtcg ctgcgtacaa
     2581 gggagggacc gacctgccac agagcgcgac gcgcgtcgac aagttcgccg ccgagctcca
     2641 gtcgaagtgg aacgtcgagt ttgtgcccga tatccccacg ctgtgcagca aagtggacgc
     2701 cattctgctc gagagcgtgg acgggcggat tcatctcaag gaggcaaaac agattatcgc
     2761 cgcacggaaa ccgatgttca tcgacaagcc gctggcggcg acgctcgagg atgctcggga
     2821 gatcgcgcgg ctggcggccg aggcgggggt gccctggttc agcagctcga gctcccggtt
     2881 tggtggaatc gccaaagcga tgaagcatgc ggacgcgacc ggtgtgctga cgtgggggcc
     2941 gggtcctctc gattccaacc tgccgctcga tctggcatgg tacgcgatcc acccgctcga
     3001 actgctgtac tcgctgatgg gtccgggctg tgaggaggtc acgcggattt ccggaagcac
     3061 cgaggaggtg ctgatcggcc gctggaaaga cgggcggatt ggcacggtcc gcgcgctacg
     3121 cgcggccggt tcttcgggcg cggtggtcta ccggccgaaa caggtggtgc agagcgatcc
     3181 ctcaatggag ggcgggtacg agggtctgtt ggaagaggta gtgaagttct ttcagacgaa
     3241 gcagccgccg gtgtccaacg cggagacgat cgagatcttc gcgttcatgg acgccgccaa
     3301 gcggagcaag gaagagggag gaaagccggt taagctgcgg taaataacga gcgggtgaca
     3361 ggcaatcgtg tccacgcgca aaaaacgctt gggacacgaa agccagtcac ccggactact
     3421 tgagcgtacg ttcgaagaac gacgtgagcg tctccaggaa atggcggcgg tagagaccgg
     3481 agagcccgtg ggtcttgagc gggtagtgca ttagctcgaa aggtttgccg gccttttgga
     3541 gcgcgtccgc catttgcagc gtgttctggt agagcacgtt gtcgtcctcg acgttgtgaa
     3601 cgagcagaag cttcgccttg agctgcgcgg cgaagtggag cggtgaactg cggcggtagc
     3661 cgtccggatt gtccgcgggc aagcccatgt agcgttcggt gtagatggtg tcgtagttgc
     3721 gccagtcggt gacgggcgcc ccagcgacgc cggcgcggaa caattcgggt gcgtagagca
     3781 gcgagtacag ggtcatgtag ccgccatagc tccagccgtg aataccgagg cgtgtggggt
     3841 cgacaaagcc catggatacg agccgctcga tgccttcctt ctgatcctcc aattcgcgcg
     3901 cgccgagatt gcggaacagc gctgtttccc accgatgacc gcgacccgct gagccgcggt
     3961 tgtccaactg ccagacgacg aagccgcggc tcgcgaggag ttgttccgtt gagggtccca
     4021 tccactcatt tacgacgtcc tgcgcgtggg ggccgccata gacgatgacg acaaccggat
     4081 acttccggtc aggacgaaag ccgacgggac ggatcagccg ggcatggaga gacgcgccat
     4141 ccgatgtccg gacgtcgacg atctgctccg gctgcagatc atactcctcg atcggagtgc
     4201 ggtcggcttc gagaaatacg gcctgctcga cgccatccat ccggaagacg gagcggcgcg
     4261 gcggcgactg gaagctcgag aacgtatcga ggtaattctt gcagccgggc gccatgagga
     4321 tggcgtgggt gccggcggtc caagtgattc gcctggtctc gccgccgctc aggggcacgc
     4381 tgtagaggtg gcgctccagc ggtgtcggcg aggttgacac gaagtacacg cggccggacg
     4441 attcgtcgac tccggcgatc tccgtcactt cccagtcgcc ttgggtgagg cggcgcacgt
     4501 catggccgtc ctcagagtgg atgtagatgt ggcggaaacc gtcgcgctcg ctggtgcgaa
     4561 ggaattcctg cccgttgcgg agaaagcgga ggtctccggc gagatcgacc cagtgggaat
     4621 cttcttcgcg caaaatcacg cggctcgggc cgttggcgat gtccgcgcgg acgagctcga
     4681 gacggttttg gattcggttc agacgctcga cgaagacgct tttcgaatcc ggcgtccagt
     4741 agacgcgagc gagcaggtcg tcgcgggaac cacccagagc gatccagcgg gtgtggccgc
     4801 cgactgccgg gacgatgccg aggcgcacgt ccgcgttggg gtcgcccgct ttgggatagc
     4861 gctgcggctc gaaaacgggg cgcagaggga gcaggtcggc atgcggatac agcggctgcc
     4921 gggtgacgtc gaactggagg taagcgatga agcgagaatc gggcgaccac cagtgcgcgg
     4981 tggagaggtc gagctcctcg gggtagaccc agtcgaggcg ggcattccag cgcgtttctg
     5041 tggcgtcgtg cgtaagacgg atcacgcgcc tcgacgcgat gtcaagtacg tggagttcgt
     5101 tgccttttcg gagggagacg cggcggccgt caggggagag ccgcggctcg tgctcagctt
     5161 cactcgtttt tgtaagctgc cggcggttgc ccccgtcgat cgggcagatg aagaggtcgc
     5221 ctccggcaag gacgagcaga ctgcgcccgt cagggaacca ctggaaggtc tgttccttga
     5281 catggcggtt cttccaccgt tcggcggcgg ctgcggcggt cggcgcttcg agatcgctca
     5341 gagaaaggag gcggcgcgtg ttcttggagg acgtatcgaa gagcgacagc tcatcgccct
     5401 gtttcacggc gaagcggtcg cccgcgggcg cccagacgat ctcgggtatc tgctccgcgg
     5461 cctcgcgcgc ggcgacaacg gcgtcgacgg tgacaggctt tttctgcgct gggcacaggc
     5521 ctgggaaaaa aacgaggagt agaacaggta agactcgcat gatgcggtgc gaatctcgat
     5581 tgtagcggtt tcggagcgcg ttctccggtt tgagagcggc cctgggagaa cttcaagcta
     5641 aatctccact cccatctcgc tccaggtcat cacctttttg cggtaaatcg cttcccggcc
     5701 catgatggaa gtcagtgcgg cgacgacgcc gacctccagg ccgcccggca cgtctgtctt
     5761 gcccgtacgg actgcctcga agaaagcctc gaagtgcagg tcctgcggcg cggagtctgc
     5821 tgactgtgac agcacgatcg gctgtgcgcc tctttcgcgc ttgtagtagg agaccttccc
     5881 ctctctgccg tacaccacgt ccacagcgcc cttggtgccg tacacctgga aaccccatcc
     5941 tccgtgaggc aggcccgttg gatgaaaggc gagctgggta aacgacatct tcacgccgtt
     6001 cttatactcg taactcagcg tgtagccgtc catgttggtg cgccctggag ggtcgttctt
     6061 ccagagcagc gtgccgccga agccggccgc ccgctcggga cggctgtcga caagccagtt
     6121 gcatacatcc aggttgtgaa tcgcattttc gacgatcaga tcgccggagc gcttggcgtt
     6181 gaaaaaccag tcggcagagg aactgttgtg atccatgtcg tcgctcgagt gccgccaagc
     6241 ccttagcatg atgacctctc ccgcaattcc ttcgcgcagg cgctcgacag ccgtcttcag
     6301 ataggggtaa gaacgcatta cctggcccgt ctgaaaaact ttcttcgagc ccttggcagc
     6361 cttcagcaac tcacgaacag attccggagt aataccgacg ggtttctcaa ggtagatgtg
     6421 cttgcctgcc cgcaaagcgg cgattgccat ctcgacgtgc agatcgcagg gcacggcgat
     6481 ccagactgcc tgtatatctt tcctctccag caacgcccgg tagtcggaaa aggtggcagg
     6541 gttatcccgg gccgccagcg tggctactct gtccaggcgg tcggccttga tgtcgcaaag
     6601 cgccttcacc ttgacagccg gcagcttgag gagggactcc accaggaatg tcccgcgaat
     6661 accgttgccg atgacaccga cgttgaccgg ctccgcatca gcttgcgcat tcgcggaacg
     6721 ggatagcgtc gccgtggtgg ctccggcgag aaagcccctt cggctgagtt cgttcatgat
     6781 ttcacctcgg agcgaggata ttctgctgcg cggtggaatg caacacccgg cggtgttttc
     6841 gcctgactgt gaagacttga attctggtgt tgctaaaact atgattcgtg acgggatttc
     6901 gtccattcgg tcgctcaagg aggccaaatg cacaggtttg tatccaggac gtttctgttc
     6961 ctggcgccct tacttgttag tgcggatacg atcgcgatca aagccggtcg gctcatcgat
     7021 ccagagacgg gaactgcagc cccgaaccaa gtgattctgg tcgaggcggg caggatcaag
     7081 tccatcaccg ctgacgcagc tgcggccaag ggatttgagg tgatcgacct ctccgggtac
     7141 tccgtgctcc cgggtctggt cgaggctcac aatcaccttg ccctcacata caagatggag
     7201 cccgagcacc tccagttcta cttgacttac gtgatggact cgaccgccat tcgagccatt
     7261 caggcgatcg cgaatggggt caccatgctg tcggcgggct tcaccgtggt gcgcgacatg
     7321 ggaaatgccg gtctgtacgc ggacacggca atgcgcgtcg caatcgagca gggctgggtg
     7381 ccggggccga cagtgatcaa ctccggcatc atcatcggcg gtttcggcgg gcagtttaat
     7441 ccaacccccg agcgggcgga actcgtgtat ccggaatacc tcgatgccga tacgcccgac
     7501 gagatcgtca aagcgattcg gcgcaacatt ctgttcggcg cgaaggtgat caagatcatg
     7561 gtggattcca agacgtacgg ctacaccgcc gacgagatca ggttgttcgt gaaagaggcg
     7621 gccaaaagcg gg
//
LOCUS       DATIFQ010000249         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_656380, whole genome shotgun sequence.
ACCESSION   DATIFQ010000249 DATIFQ010000000
VERSION     DATIFQ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656380"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..872
                     /locus_tag="VLE22_11555"
                     /pseudo
     CDS             1..872
                     /locus_tag="VLE22_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611840.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1286..2299)
                     /locus_tag="VLE22_11560"
     CDS             complement(1286..2299)
                     /locus_tag="VLE22_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HSB15087.1"
                     /translation="MKVTTIGLDIAKHVFQLHGVDEQGNVVLRRRLRRDEVAPYFANL
                     PACRVGLEACGGAHYWARRLSTLGHTVRLIAPQFVKPYVKSNKNDANDAEAICEAVSR
                     PHMRFVPAKSVEQQDIQCLHRVRSRLVGTRTQLANQVRGLLMEFGIVLPQQIQNLRGR
                     LPSILEEDNELTPFTRRLLASLYDELVELDEKLAVIKQQVRAAYKASESSQRIAAVEG
                     IGPLTATALVAALSDGKAFQNGRQFAAWLGLVPRQHSSGGKPRLFGISKRGDPYLRTL
                     LIHGARSVVYRAAGKSDDRSRWIVDKQQRLGTTKACVAVANKNARIVWALIAHNEAYE
                     RTV"
BASE COUNT          628 a          943 c          987 g          649 t
ORIGIN      
        1 gtcgccgagc tgctgctgga caaacgagcg ttgcaggcgg tggttgcaaa aaaatggtga
       61 gcccgcggct gcggaagcag gcggtcgtag tgatgcgctc ggaagtagcg gtctcggagc
      121 gacgcgcctg cgggctgatt cagatccatc gtggaacgta tcggtacagg cggcggccgg
      181 aggatggccg gctgcgggtg cggctgcgcc agctggccga agaacgacgc cggttcggct
      241 accgaaggtt gcaggtgctg ctggtgcgcg aagggtggga ggtgaaccac aagcgcgtgt
      301 accgactgta cgtggaggag aagctgagca tccgtcggaa gcgcggacgc cggcgggcgc
      361 cagccgcggc gcgggtggtg ttagcggagc ccaccgaacc ggaccaggtg tggacgatgg
      421 acttcaccca ggacgccttc gccagcggac ggcggttccg aacgctgaac ttgatggacg
      481 gctgcacgcg cgaggcgctg gagatcgagg tggacacctc gttgccgggg ctgcgcgtgg
      541 tgcgtgtgct ggaaggactg aaagcacaag gacggaaacc gaagcacatg accatcgaca
      601 acggcactga gttcaccagt caggtggtgg atcggtgggc gtacgagaac ggggtgcagc
      661 tgcacttcat cacgccgggt cggccgatgg agaacggcta catcgagagc ttcaacggca
      721 agttccggga tgagtgtctg aacgagaact ggttcctgga cctagccgat gcgcggcgca
      781 agatcgccga ctggaagtgg gactacaacc acgtccgccc acactcggcg ctcggctacc
      841 agacgcctat agagttcgca acatcgtggg cggcaacagg ccgtggaaaa gacggcggcg
      901 gagccgcctt ggaaaacgct tcgcgtttcc cactttccca ccgcctcgac ggctgcgaga
      961 aggtgactac tggtgtagtg ttggagaacc caaacccgga gaaagtctca ttatccgtgg
     1021 actaaaaatg gggggcatat atggacccat cccgattgca agagccctcc gattcctgcg
     1081 cggtggaaga gagacgcgtg catatatccg gcctcttact cggaggagct cacggctcct
     1141 cgggccctga tggactccgc gcgcttcttc tcctaatcag gtcaacggcc tttgacggcc
     1201 ctgccccccg caggttctga agaagctggt ctcagccata gcgccatcgt cggcggctca
     1261 agcatctcgt tggaagacag tggtcctaaa ctgttctctc gtaggcttcg ttgtgcgcga
     1321 tcagtgccca gacgatacgc gcattcttgt tggctacggc gacgcacgcc ttggtggtcc
     1381 ccagccgttg ttgcttgtcg acgatccagc ggctgcggtc atcgcttttt ccggcggccc
     1441 gatacaccac cgagcgtgct ccgtggatga gcagtgttcg tagataggga tcgccgcgtt
     1501 tgctaatgcc gaacagccgc ggcttgccgc cgctggagtg ctgtcgaggt acgaggccca
     1561 gccaggcggc gaactgccgc ccgttctgga aagctttccc gtcgctgagt gccgccacca
     1621 gcgccgtagc cgtaagcgga ccgatcccct cgactgctgc aatgcgctga ctcgactcgc
     1681 tggccttgta tgcggccctc acctgctgct tgatgacagc gagcttctcg tccagttcca
     1741 ccagttcgtc gtacagggaa gccagtaggc ggcgcgtgaa gggagtcagc tcgttatctt
     1801 cttccaggat cgatggcaat cggcccctca gattctggat ctgctgcggc agcacgattc
     1861 caaactccat cagcaggccg cgcacctggt tcgccagttg ggtgcgtgtg cctaccaacc
     1921 ggcttcgcac ccggtgtagg cactggatgt cttgctgctc gacggatttt gcaggcacga
     1981 agcgcatgtg aggccggctg accgcctcgc agatggcctc ggcatcgttg gcatcattct
     2041 tgttggactt cacgtagggc tttacgaact gaggagcgat caggcgaacc gtatggccga
     2101 gtgtgctgag tcggcgcgcc cagtaatggg ctccaccgca cgcttcaaga ccgactcggc
     2161 acgcaggcag gttggcaaaa taaggcgcca cttcgtctcg ccggagtctc cgtcgcagca
     2221 ccacgttccc ttgctcgtcg accccgtgca actgaaacac atgcttggcg atgtccagcc
     2281 caatcgtcgt gaccttcatg aacagccctc ctctcgatgg caatgagttg cagctgcaac
     2341 gctgctgccc tcattcttcc accgggatgg ggtccattcc attaggtcag tacgtcacgg
     2401 ttcgatggtt ccctcagctc tgctaggcta atggtcctga aaaaccgcgg attctgtggt
     2461 tagctgagct gcgcgctcga aacccggaca gccagctcaa gtgccgcaat aactggcgcc
     2521 aaaataggct tgacttccgg ccaggtgagg gcacgctgcc ggagtctttg ttccttcatt
     2581 gcgcaaccct tcgaatccgg agagttccgc ctctatcgtg caagaagaat cccttttttc
     2641 gtccttcttc ccattaggcg gctaggcgcg atcggtctcc tgtactcccc gatcgcgttg
     2701 gtgatcgggg acaccatcgg ttcgatgagg caaatctcgg gccaaagtgg tcgggtcctt
     2761 cttgtacgat tcgaaaagcc acccataaag tcgactgtga ggattcagcg agaacatagc
     2821 cggtacttgt aggatcacta gcgttcggcc cggatctctg gatcgcagcg gtgccaacaa
     2881 ttttgcattg tgttcaagcg cgatatcgac agagcgagct aacaatgtta ttggtgattt
     2941 cctgcttacg accgtgatcg acgccctacc ttcattctgc gcttgcacgg gcgagcctct
     3001 gtaagccatc gacgaaggta aatccccaaa caatcggacc cgctcccctc tatacgattt
     3061 aggcgaaaat gccgcgacaa tccagacgct tgctgtccta tgttgcggat tgacacgtgc
     3121 agcgcccagg catattcttg gagcagctga gtacggcccc gggtggaagc ccggtattca
     3181 gaaaccatcg gtatagcgcc gataaac
//
LOCUS       DATIFQ010000250         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_464383, whole genome shotgun sequence.
ACCESSION   DATIFQ010000250 DATIFQ010000000
VERSION     DATIFQ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464383"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /locus_tag="VLE22_11565"
     CDS             complement(<1..1898)
                     /locus_tag="VLE22_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HSB15088.1"
                     /translation="MKYGFLVFLLPVVMHAQNTGFVVDNDATEGRVQVIDLASNPRKV
                     IASIPAGRQPSELLILPDNRFGLVTTYLDNNVTLLDLRENKPLATMATGEGPGSLTCS
                     PDGRFVYVANDTSNDVTVIDVAARAAMATIPVGVTPVQVNISANGRFAYAVNQDDGTV
                     SVIDTNRNTVVKTLTVGTKPNQFAILPGLGNAYVVNTGSNDVSVVNLTTNEVVGDPIR
                     VGQAPVTVAFSSDSKKLYVVNRDSNGVSVIDTVQNKVTATIPVGTQPVAMVVTFDNQY
                     GYVSNQGSNDVSVLDLGSEVRTNDSIIAVGTKPFSLMLDPNEEYLYVANLGPLGTVSV
                     IDINVDKVVNTIQVGGTPVQFTMRNAPTLLEIAPNPAPAGSTITLSGESFVDGSLVRF
                     FVGNRTVTASPSFLDSQGLTVTVPAGASNAVVDVLHPDGNSSERLTLRLGTAAASIFA
                     GGVVEGAGFAKAPEPISGGAFVSVFGTFPGMEDATAGAFPLPTLLGNTVVTFNGVPAP
                     LFATVPAAGQINLVAPIRLLARSQVRVAATVSGQTSAAETVNVAPLSPGVFFDPASGD
                     GAFLHADYSLVTPANPVRKEAKEPPDTILLYMTGLGNTAPPWTDGEMPAQDVLTTTVA
                     APEVTVGG"
     gene            complement(1895..2785)
                     /locus_tag="VLE22_11570"
     CDS             complement(1895..2785)
                     /locus_tag="VLE22_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15089.1"
                     /translation="MRKMGTDTISPKNGDRLHFAVVAKWLSVPIFLTSLSPSFPLFLV
                     CLLSVSVQAQQKRRVSAPISFYDDAEISMPGVLNVSEYFSYTKVPAGRDLSFPSTYLA
                     LGLNRRVGVEGSFSYASSQFEESRINALGDSSVAVKLLVFPEGKRWPALALNPMLEVL
                     GDASIADNPLAPGRVNYVLPLVFQKSFDYYRIYSMAGYLTRGIVFDSTVFELNRWSRV
                     TPLVIVSGSRLTHELKLISDLGLNRSRMDVTGGVAVALKPGVAVFVNTGRSLGRVDLN
                     STRYQVTFGLSFNVRLWGEK"
     gene            complement(2782..>3525)
                     /locus_tag="VLE22_11575"
     CDS             complement(2782..>3525)
                     /locus_tag="VLE22_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15090.1"
                     /translation="NVMPDRHVGGTSLPSARATGSELALTVLAAASASGRAALYSEYT
                     VASQDWSLIGAALAHRARIETRGGGWEVESPTPLRLVAYEDRDYYRGGRLWPAVWVDG
                     VIAPPGRYRLASQRPWYQFLDRGELPARLLHLSADLLEASVKSTGLDVRYRSPGRAVL
                     VMNQKPIAIRINGRDAEAPVEEGEGRWALLLPRGEQEVEIETASRAGVFVNVWSYVSA
                     SAITAFGALTTVLMAAIYLRLRFRRGGAS"
BASE COUNT          690 a         1096 c         1139 g          600 t
ORIGIN      
        1 tgcccaccta ctgtcacttc cggagcggca accgtggttg tgaggacatc ctgggccggc
       61 atttcgccgt cggtccacgg cggtgcggtg ttgccgaggc cggtcatgta cagcaggatt
      121 gtatcgggtg gctcttttgc ctccttccgt actgggttcg cgggcgtcac gagcgagtag
      181 tcggcatgaa gaaacgcgcc gtcacccgag gcaggatcga aaaagacgcc aggagacagt
      241 ggcgctacgt tcaccgtctc cgccgcggag gtctggccgc tgaccgtcgc cgccacccgc
      301 acctgactcc gcgcgagcag ccggatcggc gcgacgaggt tgatctgacc agccgcgggg
      361 acggtggcga acagcggcgc ggggacgccg ttgaacgtaa cgacagtatt gccgagcaag
      421 gtgggaagcg ggaaggcgcc ggccgtggca tcctccattc ctggaaatgt cccaaagacc
      481 gagacgaacg caccgccgct gattggttca ggagccttcg cgaagccggc gccctcgacg
      541 actccgcccg cgaagatgga tgccgcggcc gttccaagcc gcagcgttag ccgctcggag
      601 gagttgccgt ccggatgaag cacgtcaacg accgcgttcg aggcacccgc cggtacggta
      661 acggtcaggc cctggctgtc caaaaacgag ggagacgccg ttaccgtacg gtttcccacg
      721 aagaaccgca cgagcgaacc atcaacgaac gactcgccgc tcagcgtgat cgtgctgccg
      781 gcaggggccg gattcggcgc gatttcgagc agcgtcggcg cgttccgcat ggtgaattgc
      841 accggcgtgc cgccgacctg gatggtgttg acaaccttgt ccacattgat gtctatcacg
      901 ctgacggtcc ccagcggccc caggttggcc acataaagat actcttcgtt gggatccagc
      961 atgaggctga acggcttggt cccaacggcg ataatcgagt cgttcgtgcg cacctccgag
     1021 ccaagatcga gcacgctgac gtcgtttgac ccctggttgc tgacgtaccc atactggttg
     1081 tcgaacgtca ccaccatcgc caccggctgc gtgccgacgg ggatagtcgc agtcacttta
     1141 ttctgcacgg tgtcaatcac cgagacgcca ttcgagtcgc ggttcaccac atacagcttc
     1201 ttgctgtccg aggagaacgc caccgtcacc ggcgcctggc cgacgcggat gggatcacca
     1261 accacctcat tggtggtcag gttcacgacg gacacgtcgt tcgagccggt gttgacgacg
     1321 taggcgttgc ccagcccagg gaggatagcg aactggttcg gcttcgtgcc gacagtgagc
     1381 gtcttgacca cggtgttccg gttggtgtcg atgacgctga cggtgccgtc atcctggttc
     1441 acagcgtaag caaaacgccc attcgcggag atgttcacct gcaccggcgt aacaccgacg
     1501 ggaatcgtcg ccatggcggc gcgcgcggcg acgtcgatga cggtaacgtc gttgctcgtg
     1561 tcgttggcga catagacgaa ccgcccatcg ggggaacacg tgaggcttcc ggggccttcg
     1621 cccgtcgcca ttgtcgccaa cggtttgttc tcgcgaaggt ccaacagagt cacgttattg
     1681 tcgaggtagg tggtaacaag gccgaaccgg ttgtccggca ggatcagcaa ctcagacggc
     1741 tggcggccgg caggaatcga tgcgatcacc ttcctgggat tcgaggcaag atcgatcact
     1801 tgaacccgtc cctcagtggc gtcgttgtca accacgaagc cggtgttctg ggcgtgcatc
     1861 acgaccggga gtaggaacac cagaaagccg tacttcattt ttcaccccac aagcgcacat
     1921 tgaaactcag tccaaacgtc acctggtagc gggtgctgtt caggtccaca cgtcccaggc
     1981 tgcggcccgt gttcacaaac acggccactc ccggtttgag agcgaccgcc acgccgccgg
     2041 tcacatccat gcgggaacgg ttcagcccga ggtcgctgat cagtttcaac tcgtgcgtga
     2101 gccgcgaacc ggagacgatc accaacggcg tgacccggct ccagcggttg agctcgaata
     2161 ccgtcgagtc gaagacgatc ccgcgcgtca ggtagccggc catcgagtag atccgatagt
     2221 agtcgaagga cttctggaac accagaggaa ggacgtagtt cacgcggccg ggcgctaacg
     2281 gattgtcggc gatgctggcg tcgccgagca cctcaagcat gggattcaga gccagcgcgg
     2341 gccaccgctt gccctccgga aacaccagta gcttcaccgc aacggaagaa tccccgagcg
     2401 cgttgatccg gctctcctcg aactgcgagc tggcgtaaga aaaactgccc tcgacgccga
     2461 cgcgcctgtt cagtccgagg gcgaggtagg tggagggaaa agagaggtcg cgtcccgccg
     2521 gcaccttggt gtaggagaag tattcgctta cgttgagcac gccgggcatt gagatctcgg
     2581 cgtcatcata aaaggagatg ggagcggaga cccggcgctt ctgctgagct tggacggaga
     2641 cggagaggag gcagaccagg aacagcggga aagatgggga gagagatgtg agaaaaatgg
     2701 ggacagacag ccatttcgca acgacggcga aatggagtct gtccccattt ttcggcgaaa
     2761 tggtgtcagt ccccattttt ctcaagacgc gcctccgcgg cggaagcgca ggcgcagata
     2821 gatggccgcc atcagcacgg tggtgagcgc gccgaacgcc gtaatcgccg aggcggaaac
     2881 gtaactccag acattgacga acacgccggc gcggctggct gtctcgattt cgacttcctg
     2941 ctcgccgcgc ggcagcagga gcgcccaccg gccctccccc tcttcgaccg gcgcctcggc
     3001 gtcccggccg ttgatgcgga tggcaatcgg cttttggttc atcacgagta ccgcgcggcc
     3061 agggctgcgg tagcgaacgt cgagaccggt ggatttcacg ctcgcctcca gaagatccgc
     3121 gctaaggtgg agcagccgcg cgggcagttc tccgcggtcg agaaactggt accacggccg
     3181 ctgcgacgcc agccggtagc ggccgggcgg cgcgatcacg ccatccaccc aaacagcggg
     3241 ccacagccgg ccgccccgat agtagtcgcg gtcttcgtac gcgaccagac gcaagggggt
     3301 ggggctttcc acttcccagc caccgccccg ggtctcgatg cgcgcccgat gggccaacgc
     3361 cgcgccgatc aggctccagt cctggctcgc aacggtgtac tcggaataga gcgccgcgcg
     3421 tccggaggct gaagcggccg cgagcacggt aagcgcaagc tcggagccgg tggcgcgcgc
     3481 ggacgggagc gacgtgccgc cgacgtgacg gtccggcatc acgtt
//
LOCUS       DATIFQ010000251         3069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1997842, whole genome shotgun sequence.
ACCESSION   DATIFQ010000251 DATIFQ010000000
VERSION     DATIFQ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997842"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VLE22_11580"
     CDS             <1..1159
                     /locus_tag="VLE22_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSB15091.1"
                     /translation="KDPGPWLREEPRKRGEIFREFPPHRRMYNDLYSHPNFDNYWKQR
                     GFYTAGYYREMKDVPMLFLTGWYDYFGPGSIGSFAALSRIQKTPKKLIVGPWPHGIGG
                     RECGDASFGDSAGFDMRAMMAAWFGQWLRGDPPAAGTEPVRIFRMGGGDGARTASGKL
                     NHGGEWRTAAAWPPPAASPVRYHLQTGGGLGTEAPAGTAAPSSFVFDPANPVPTIGGR
                     YALGAVTPLCAQNQVCSPTILGCSDSKPLKERPDVLSFSTPPLESPVEATGTIRAKLW
                     ISSDAEDTDFTAKLVDVYPSGYALILTDGQIRARFRKSFERPAPLKPGKVYEVTIDLG
                     PTSNLFAPGHRIRVDISSSNFPRFESLTRKARNVVYHDARRPSYIELPVIR"
     gene            complement(1168..1968)
                     /locus_tag="VLE22_11585"
     CDS             complement(1168..1968)
                     /locus_tag="VLE22_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15092.1"
                     /translation="MKRWVFCIAVVLGLFTTGAEAQDQPSGYHRVACIKVRPGKGAEF
                     RQFNADAVRRFFQASADSGEIAGWYLLRTVIPAGSEAACDYVAVTLFTGLPRPPLGLE
                     EIDKGLKRAGVAMSGSEFVAKREEVSSLVSMNLSRQTVRAGAPPEKGDYAYVNFMKVH
                     SMQDWMDMENNVWKPMAASFAKDGGLRGWSVNVLVLPSGTDTKFQAVTLDVFPSWEAV
                     FKPLPVEDTFKQVHPDKDAEQTFEKLLKSRDLARRELLVFEEKVVPAR"
     gene            complement(2089..2868)
                     /locus_tag="VLE22_11590"
     CDS             complement(2089..2868)
                     /locus_tag="VLE22_11590"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HSB15093.1"
                     /translation="MRIPLYQIDAFTSRLFSGNPAAVCPLEAWLDDSVLQSIAAENNL
                     SETAYFVRNGGRYDLRWFTPTVEVDLCGHATLASAFVILNYIDSSAATVAFDTRSGEL
                     IVRRQDDRLSMDFPSRPPEPCETPGKLAEALGRQPAEILASRDYLAVFETEADVRAVR
                     PDMDLLAQLEQFAVIITAPGKEVDFVSRFFAPRGGVPEDPVTGSAHCTLVPYWTKRLG
                     KKKHHAWQVSARGGELWCEDRGDRVTIAGYAVRYLEGFIHL"
BASE COUNT          562 a          942 c          988 g          577 t
ORIGIN      
        1 gaaggatccg gggccctggc tgcgcgagga gccccgaaaa cggggagaga tcttccgcga
       61 atttccgcca caccggcgaa tgtacaacga cctttacagc cacccgaact ttgataatta
      121 ctggaagcag cggggcttct acacggcggg ttactaccgc gagatgaagg acgtcccgat
      181 gctgttcctg accggctggt acgactactt cgggccgggt tcgatcggga gtttcgcagc
      241 gctctcgcgg atacagaaga cgccgaagaa gctgatcgtg gggccgtggc cgcacggcat
      301 cggagggcgc gagtgcgggg acgcgtcgtt tggtgattcg gccgggttcg acatgcgcgc
      361 gatgatggcg gcctggttcg gccagtggct gcggggagat cctccggctg cgggcacgga
      421 gcccgtgcgg atctttcgca tgggtggtgg cgacggcgcg cggacggcgt cgggaaagct
      481 gaatcatggc ggcgagtggc gcaccgcggc cgcgtggcct ccgccggcgg cgtcaccggt
      541 ccgttatcac cttcagacag gcggaggatt gggcacggag gcgccagcag gaaccgccgc
      601 gccctcgagt ttcgtcttcg atcccgcaaa tcccgtgccg acgatcggcg gccgttatgc
      661 gcttggggcg gtgacgccgt tgtgcgcgca gaatcaggtg tgttcgccga cgattctcgg
      721 atgcagcgat tcaaagccgc tgaaggaaag gccggacgtg ttgtccttct cgacaccgcc
      781 gctcgaatcg ccggtcgagg cgacaggcac cattcgcgca aagctgtgga tctcctccga
      841 cgcggaagat acggatttca ccgcgaagct ggtcgacgtc taccccagcg gctacgctct
      901 catcctgacc gacggacaga tccgtgcgcg attccggaag agctttgagc ggccggcgcc
      961 gctgaagccg ggcaaagtgt acgaggtaac gatcgacctc gggccgacaa gcaacctgtt
     1021 cgcgccaggg caccggattc gcgtcgatat ttcgagcagc aacttcccgc gcttcgagtc
     1081 gctgacccgg aaggcgcgca acgtggtgta tcacgatgcg cggcggccgt cgtatatcga
     1141 actgccggtg atccgctgac cttggaacta ccgtgcggga acgaccttct cttcgaacac
     1201 gagaagctcc cgccgggcca ggtcacgaga cttgaggagt ttctcgaacg tttgctcggc
     1261 atctttgtcg gggtgtacct gcttgaacgt gtcttcgacc ggcaggggct taaacaccgc
     1321 ctcccaactt ggaaacacat ccagcgtgac cgcctgaaac ttcgtgtcgg ttccggacgg
     1381 cagcaccaga acgttcacac tccaccctcg caggcctccg tccttcgcga acgaagcggc
     1441 catgggcttc cacacgttgt tttccatgtc catccaatcc tgcatgctgt gaaccttcat
     1501 gaagttcacg tacgcgtagt cgcccttctc tggtggcgcg ccagcgcgaa ccgtttgccg
     1561 agagaggttc attgaaacga ggctcgacac ctcttcgcgc ttcgcgacga attcgcttcc
     1621 agacatcgcg actcccgcgc gcttcaaccc tttgtcgatc tcctccaggc ccaaaggagg
     1681 gcgaggcaga ccggtgaata aggtcacagc gacgtaatcg catgccgcct cgctgccagc
     1741 gggaattacg gtgcgcaaca ggtaccagcc ggcgatctca ccgctatcgg cgctcgcctg
     1801 gaagaatctt cgcacggcat cggcgttgaa ctgtctgaac tcggctccct tccccggtct
     1861 tactttgatg caggcgactc tgtgatagcc acttggctgg tcctgagctt cagcaccagt
     1921 cgtgaacaac cccagaacaa cagcaataca aaacacccag cgtttcatgg atcccctcct
     1981 taatgagaat catgcccggt tccacagggg atcgcccgcc actgagttgc agcaggctat
     2041 cacggacggg gtgtgggagt caattcgtct cgtgtcgtcg tcgtacccct acaggtgtat
     2101 gaagccttcg aggtagcgga cagcgtaacc cgcgatggtg acgcgatccc cgcggtcctc
     2161 gcaccacaac tcccccccgc gggccgagac ctgccaggcg tggtgcttct tcttgccgag
     2221 acgcttcgtc cagtaaggga ccagcgtgca gtgtgcggaa ccggtgacgg gatcctccgg
     2281 gacgccgccg cggggcgcga agaagcggga aacgaagtcg acttccttgc cgggtgcggt
     2341 gatgatcacg gcgaactgct cgagttgcgc gagcaggtcc atgtccggcc gcacggcgcg
     2401 cacgtcggct tcggtttcga agaccgcgag atagtcccgc gaagccaaga tctccgcggg
     2461 ctggcgcccg agggcctcgg ccaatttgcc gggcgtttcg cacggttctg gcggacgcga
     2521 cgggaaatcc attgataggc ggtcgtcctg gcggcggacg atcaactcgc cgctgcgagt
     2581 gtcgaacgct acggtggcgg cggaggaatc gatgtagttc aggatgacga aggccgaggc
     2641 gagggtggcg tggccgcaga ggtccacctc gaccgtcggg gtgaaccacc gcaggtcgta
     2701 gcggcccccg tttcgaacga agtatgcagt ctcggacaga ttgttctcgg cggcgatgga
     2761 ctggagtacg gaatcatcga gccacgcctc gagcgggcaa acagccgccg ggttgccgga
     2821 gaagagacgg ctggtgaagg cgtcgatctg gtaaagcggt atccgcatat ccccatgtta
     2881 atttggatac ggcggtcgtg ttccgatgag acgtttcact ctccttctgc tttttgccct
     2941 ttctgctgcg gcacaaacgc cgctctcgct gagggacgcg gtatcgcagg ccctttcgat
     3001 tcatccgctg ctctccgcgg gcgccgggcg tgtggcggca tcggaaggac tgcggcggca
     3061 ggcggcgct
//
LOCUS       DATIFQ010000252        42479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2572278, whole genome shotgun sequence.
ACCESSION   DATIFQ010000252 DATIFQ010000000
VERSION     DATIFQ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 42479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42479
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572278"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..775)
                     /locus_tag="VLE22_11595"
     CDS             complement(182..775)
                     /locus_tag="VLE22_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15094.1"
                     /translation="MKGRTLSRVGYLLGVLLAVSAMSFAGTSKATVQARPAKASSTWD
                     IHKEATDLLSGIETDSQHVRSLADELEVLRLHLTVGWDVHGQKLEEIRQAVNDMGTKL
                     SRLETIRSSAFPWQQRVIDHVAPLVQLMADNAQDAILFLNANQQGLWKPEYGKYTRNM
                     VAEADQIATSVHEFETYASARQTEMQLGKDLGMNSGS"
     gene            complement(919..1569)
                     /locus_tag="VLE22_11600"
     CDS             complement(919..1569)
                     /locus_tag="VLE22_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB15095.1"
                     /translation="MASIRILLADDHTVMRAGLRLLLERQPDFEVVAEAEDGRRGVEL
                     ADVHAPDVVVMDIAMPRLNGIEATRQIAIRHPQTAVVILSMHVDESYVMRALKAGARA
                     YLLKDSAEVDLIGAVRAVALGKSFFSPAVSKLLAEDYVRQLERKGEVDSYELLTARER
                     EILQLLAEGQSNKDVANLLNLSLYTVETHRSHILQKLGLHTIPDLILYAVRKRIIR"
     gene            complement(1574..2977)
                     /locus_tag="VLE22_11605"
     CDS             complement(1574..2977)
                     /locus_tag="VLE22_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSB15096.1"
                     /translation="MGSSEKIKGSKETPDEDQPSRSGVRLVSLVGFGAVLALMGIIGA
                     ESVRVLHSIESENVEVTRDYLSRHHALDRVRSSLYLSGTLVRDYLLDPDQGRARSRLA
                     ELQEVRAQTNAALDEYRGDGLSEDVPQLATLRAELAEYWKTISPSFDWDAGQRRSLSY
                     RFLEQLVLPRRDAMLRLADRVDGMNEAVLKAGGLRSAEIYRSFRSRIVAILTITLGLG
                     IGVAGLSIMQILRLERDARLRYQETVRARSELENLSAKLVDAQEEERRTISRELHDEV
                     GQSLSALLVDLGNVVATVPEENVPARRLLDTVKKLAEQSLQSVRNRALLLRPSMLDDF
                     GLVPALRWQTREASRRTGTIIELAAEETADELPDDYKTCVYRVVQEALNNVSKHSNAQ
                     SVQIVVRQEKNRLLLSIQDDGKGFDALHTRGLGLVGMEERVRHLGGVFVIQSKPGQGT
                     HLRVDLPLRGESSGLYG"
     gene            complement(3016..3489)
                     /locus_tag="VLE22_11610"
     CDS             complement(3016..3489)
                     /locus_tag="VLE22_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15097.1"
                     /translation="MLDTTRRKAATPIPARELGGSAYGAKIDCVFVTPWARSVAEATR
                     ALRRSGIWVHHADTIERARAMLGLTGAKVLLAEASFRDGNWQDAVALSEEFAPPVSVV
                     VVTTVVDERFWITAIENGAYDLVSRPFSSIEFAHIVENAYSHAWALRGVHRSGSA"
     gene            complement(3496..3882)
                     /locus_tag="VLE22_11615"
     CDS             complement(3496..3882)
                     /locus_tag="VLE22_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSB15098.1"
                     /translation="MSESELKRIVEMLRGKQRELRLEAPRIDDIAVERVPDEIDAIQF
                     AVDREMAIGGLERRTELSHEVDAALKRIAEGTYGRCEGCGGAISLGRMLVVPWASLCI
                     HCQEEEDSSQGGRWYRERGRVLDDAA"
     gene            4040..5329
                     /locus_tag="VLE22_11620"
     CDS             4040..5329
                     /locus_tag="VLE22_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSB15099.1"
                     /translation="MTRIPILWLVLALQVLPLLSENFTGIKPSPQQVAWQDLEIGVLI
                     HFGPNTFMDREWGDGTADPKIFNPLQLDAEQWATAAKASGARYFVMVAKHHDGFCLWP
                     SRHTDYSIKSSPWRGGKGDLVREVADACHRQGLKFGIYLSPWDRHEPSYRDNKAYDRY
                     YEAQVLELATGYGEVVEFWLDGAGSEGHVYDFESYAGTLRTFQPNTLFFAGVGFLPWA
                     DIRWVGNEDGSAFEDNWNVIDLRGHLRWRPAEADTPLREGHWFWHPNDEKSLKPLDKL
                     LDVYHKSVGRGAQLVLGLAPDNRGLMPDADVAHLREFGDAIGRIYSTDLARGKTVWTP
                     PSDMHTITGEVDFRVPVRFDRTVLAEWIHLGQHVQRYDIQRWDGSRWETVHEGTTIGH
                     KKIDIFPRTTAIRMRVRILQATETPRIRGFQVFDGAR"
     gene            5350..6543
                     /locus_tag="VLE22_11625"
     CDS             5350..6543
                     /locus_tag="VLE22_11625"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB15100.1"
                     /translation="MEPGERLTRRDLVRVLGVSLGGLPLAKAAGYSPSLSAGALGLPG
                     LFPGRVIGVEHRGSLLSGNYHGPVIEQMFRRGMTELTGTPAWQEAWRLFFQPGDVVGI
                     KVNPAGQPHIISSPEVLWQIVQGLEAAGINRNDVVVYDRYREEFLSAGMDRWVPQGVR
                     TMFATAHWDDIQQTIDGYDPDHFMEMAIGMPGQNVNDSAVRRSYAARFITKAVNKLVN
                     LTTLKDHQAAGVTLALKNLSHGFVNNVNRSHPDYTRNYTGAFIPAAVSIPAIRNKAVL
                     HIIDGVKGLYHSGPHAQPQFLWENCTLYFATDPVALDRVGWTVIDQKRVSVGMKPVQL
                     APADEYSHWQNRQPEHIEIAGMMGLGEWDPSRIYHRHIRLDVPASPRSRGEGRIALPM
                     PGRDR"
     gene            complement(6540..7463)
                     /locus_tag="VLE22_11630"
     CDS             complement(6540..7463)
                     /locus_tag="VLE22_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB15101.1"
                     /translation="MRITALLLFCSMFLGAQEARRPRILGVAHIGVYVSDIEKSRGFY
                     KDFLGFGEPFGINNPDGSVMFTFIKVNDYQYIELFPGLKPDQDRLSHIALHTDDAEAM
                     RAYLASRGFKVPDKVPKGRTGNSNFSVRDPDGHDVEFVEYEPDGMAMRTKGQFLGDER
                     ISPRMRHLGILAGDLEASAGFYRDVLGFTETWRGSGNGKVLSWVNMKVPEGDDYIELM
                     LYEKLPEPDLRGSAHHICLETTDIPKSLGVLRGRAPRAGYLRALEPRVGTNRKRQLNL
                     FDPDGTRTELMEPVTVDGKPTPPSDAPPPKR"
     gene            complement(7454..8410)
                     /locus_tag="VLE22_11635"
     CDS             complement(7454..8410)
                     /locus_tag="VLE22_11635"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSB15102.1"
                     /translation="MLAVHIKDGKVDVVRKPRPRRREGHALIRLVCGGICNTDIELRR
                     GYYDFRGTPGHEFVGEVIETDNPALAGRRVVGEINLACGRCDWCRRGLGRHCPNRTVL
                     GIVRHPGAFSEFFTLPEGNLRVVPSSVSTQEAVFTEPVAAACEILDQVRVPRDSEVAV
                     LGDGKLGLLVAQVLQAHGAAVHLYGRHPVKMKIAEAAGVEARRAGAKPPRARYAWVVE
                     ATGSPEGLTEAVRMTRPRGVVIMKSTVHGTVSVDTAPVVVNEITLIGSRCGRFEPALR
                     LLKSRKLRLSEMISDCFPLSEAPRAFDRAEEKEILKVLLVCE"
     gene            complement(8458..9852)
                     /locus_tag="VLE22_11640"
     CDS             complement(8458..9852)
                     /locus_tag="VLE22_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15103.1"
                     /translation="MMAAVAFLVSIATGWLVVDTLLRPVSLRPRWAARVLTIALGAGI
                     GAGATSVLYFLVRSAGLAGRGLLFAADLMLFLAGGAVWLVFRDTARPVESPEKPGFRW
                     NAALAGALTVGFLLVVLAFLSNTEARIHGDWDAWSTWNVRAKFLAADGEEWRNAWSPL
                     LAKTHPDYPLLLPGFVARAWRYGGKETPPAVPAAAAFLFFAGTFAVLVSGFAVLRSTS
                     LGLLAGCILLASTGFLSQGTSQYADVPMGFYILGTVVLLSLTHEAAECRANVLALAGA
                     TASLAAWTKNEGLLFLACSVAGICIAQPRSRRVRTTARLLAWFIFGSLPGLLVTLWFK
                     LALAPNVNPLTKQSVFEAMSRLAEADRYFTIARALLSELLMLGDPWTHPLLLLAILAA
                     TLRFIPEPERRGYPLVAEVTLALLVAGSAGAYLTTPSRLSISLGRIFAQVWPAAILLV
                     LAALRPPEATTTTT"
     gene            complement(9854..10264)
                     /locus_tag="VLE22_11645"
     CDS             complement(9854..10264)
                     /locus_tag="VLE22_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15104.1"
                     /translation="MRVLSGVGLVAVLSLAASLEYYRTTTLTNRTAGDPYAVEIQVQR
                     LREAATILPRDVLVGYVSDVPFEQVRGSAAFFGAQYALAPRILVELPARESTDWVLGN
                     FSKPAEVSAVERRHQLKLARDLGSGVLIFRRIGR"
     gene            10302..11678
                     /gene="thrC"
                     /locus_tag="VLE22_11650"
     CDS             10302..11678
                     /gene="thrC"
                     /locus_tag="VLE22_11650"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSB15105.1"
                     /translation="MEQIDENSRTGGVDAHPPVIIKASMQAELVCFSAGCRARLPLTE
                     VIYNCPRCGGLLECDYPGRAVDTLALKRTWRERRMSNAPLDRSGVWRYREMIPFLESY
                     DKVVTLSEGNTPVLNAPHAAAWVGLEQIAFKHQGFNPTGSFKDNGMTCGVAQALRLGM
                     KRVACVSTGNTSASMAAYASAAGLQPFIFIPHGNISYGKLAQALEYGAKTLQVEANFD
                     QILALVRVLAERLGIYLLNSINPFRIEGQKTIMVELMDQRDWNPPDWIVLPGGNLGNT
                     SAFGKALREMLDLGLISRSPRLAVIQAEGAAPFYGLMHSSDRSDLPKVENPETLATAI
                     RIGDPVSWPKALHEVETSGGIVEKVTEQEIADAKAQIGLCGIGCEPASAATLAGIRKL
                     RAAGVIARNVDVVAVLTGNLLKDPDYIYRYHTGQLVGPSGQKLESTFGNQPVVVPNDP
                     DRIAEMLA"
     gene            complement(11699..12925)
                     /locus_tag="VLE22_11655"
     CDS             complement(11699..12925)
                     /locus_tag="VLE22_11655"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSB15106.1"
                     /translation="MTTITHLECSLCGKQLEAGRVHNVCECGGPLSVRYDLVQVRQGW
                     SREWLNNAPPTMWRYAAVLPVGKPGSIVSLGEGMSPLVRCRNAGARLGLEDLWVKDDG
                     VNPTGSFKARGLSCAVSMAVELGIRKLAIPSAGNAASALAAYGAAAGIEAHIFMPRDV
                     PQSNYVECMAFGAHVTLVDGLISDCARLVAEGRSTEGWFDMSTLREPYRVEGKKTMGY
                     EIAEQFHWELPDVILYPTGGGVGLLGMWKAFEEMEAFGWVSGKRPRMVAVQAEGCRPV
                     VEAFERGESRCRFWENACTAASGLRVPKPLGDFLILDILRASGGTAVSVTDAELLDEG
                     IRFAAEEGLFMAPEGAACIAALEKLIKAGAVKPNEKILVYNTGAGLKYLEAYSTRFPR
                     SAAAEQDKLGGLITPR"
     gene            complement(12948..14918)
                     /locus_tag="VLE22_11660"
     CDS             complement(12948..14918)
                     /locus_tag="VLE22_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HSB15107.1"
                     /translation="MFPRTILALLVLAPLLIAQDWRDAGVLDLRKSPHARLHSVPVRA
                     VTIEDGFWSRRRQVNVERSIPSLLALLEENGVVDNFRRLSGRKNVTRRGPLYTDSDLY
                     KWMEAVAFVLQSVDNPKLRAQFDQLTDEILAAQEASGYLNTYFVEDRVPLRFQQMQSG
                     HELYCLGHLLQAAVAYHRATGNRRLLDGALRFVEYLIRDFGPNKRPLLTGHPELELAL
                     VELYRTTGDRRHLDLAGYLVRGDGERLKLSRRDIVYLFSGKPFTERTKLEGHAVRAMY
                     AVSGATDYFIETGDEAYRRTLDRLWQDMTRGKMYITGGVGSRAQGEAFGEPYELPNRL
                     AYTESCAAIGNMIWNWRLLAATGEARFADEIERALYNGVNSGMSLDGTLYCYRNPLEL
                     TGDPEDKIRNPWYSTTCCPPNLQRVLASLPGYFYSTSREGLHIHLYDNNTLDWRLEDG
                     TGLRLKQTTKYPWEGKVEIAMRLERAVEFTLFLRVPGWASPARAAVNGRPVRDPMKAG
                     TYLAIRRKWQPNDRVVLDLGIAPRLTSANPLVRENAGRVAVERGPLVYCMEQADQYGV
                     ASIFDVKLLREGGRAKGFTGEYRRDKLGGIVELWHAGVAEEPPLTERWLYEALRDVRS
                     RVGRPVTVSFIPYYAFSNRGPSAMQVWIPYEQ"
     gene            complement(14934..16958)
                     /locus_tag="VLE22_11665"
     CDS             complement(14934..16958)
                     /locus_tag="VLE22_11665"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HSB15108.1"
                     /translation="MTQFKCWTFRARRNGIVVTLLACTILAGCQRRETAPAAIVHWNR
                     NPLTPNAMAPLPLGSVKPQGWLRQQLQIQANGLSGHLDEFWPDLGPNSGWLGGSGESW
                     ERGPYFLDGLVPLAYLLEDQRLIAKAKKWVDWTLENQRETGAIGPDPRKGKYAREWQA
                     TDWWPNMVMLKVLTQYQEATGDPRVVPLMHKYLRYHLEHAAERPLVQWASYRWAEEIL
                     SIIWLYNRTGDKELLELARALRPQAFDWKTHFADFRYPAKVTKGQISLKTHVVNNAMA
                     LKTSAVWWQISGDETDRNAIYTLLDHMDRSHGQANGVHSGDEHYAGLDPTQGTELCAV
                     VEGMFSLEQLLAILGDPAFGDRLERIAFNALPATFKPDMWAHQYDQQVNQVMCSIDKK
                     RNWTNNGPDANIYGLEPNFGCCTSNMHQGWPKFAAHLWMATRKEGLAAVAYAPSRVEA
                     RVRGGVAVVIEEDTEYPFREAIRLTVRPAKPVAFPLTLRIPGWAKDAAITVAGKRTNG
                     VKPGTFHTIERRWQAGDTVEILFPMPVRTETRYRGTVAVLRGPLVFSLKIGEEWRKVK
                     GQEPHADWEVHPTTAWNYGLAVDPENPAASISMEERPVGPLPLSPDGAPVVLSAKGRR
                     IPEWKLIAGSAGPMPESPARSAQTEETVTLIPYGSTNLRLTLFPRVASGK"
     gene            16968..17618
                     /locus_tag="VLE22_11670"
     CDS             16968..17618
                     /locus_tag="VLE22_11670"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSB15109.1"
                     /translation="MGVVYLRRLLIWVSIAIPALAGEYATLSTGFCLYVERHETAGDR
                     VRLYTGQGVTELPVSQVLAFEPEDAIPPPPSSAAPKESPSPKELVTQAALRHGLPPEF
                     VHSVAATESSYRQQAVSPKGAVGVMQLMPATARELQADPSDVGQNIDAGVRYLRDLLV
                     KYQNDDYQVRKALAGYNAGPGAVDRYNGIPPYPETQRFVEKVIRRHKRLAAEPTVQ"
     gene            17749..19002
                     /locus_tag="VLE22_11675"
     CDS             17749..19002
                     /locus_tag="VLE22_11675"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HSB15110.1"
                     /translation="MNSRFKFAFVSFSACLVVLLLVGALVGKSAPSDVPAYRQMSVYM
                     EVLSKIKSDYVEEPDLKSVTLGAMNGLLESIDPFASYLNAEQYKQYLKNSDAKKGDVG
                     LVLSKRYGYVGVVSALPDSPGAKAGLTTGDLIESINGVATRDMPLAYAELLLHGEPAT
                     DVEISILRMRSPEPQKITLTRALVSVPPVTSRMLADQVGYVRPETLVTGKSAEVAAAI
                     RTLQQQGAKGLVLDLRQCAVGTAEEGLALANLFLEKGLMAYLEGQRVPRQNFDAKSEK
                     ALTRLPLVVTTNRGTADGAELAASALIENKRAEVVGERSFGDASVRRAISMEDGSAII
                     LSVAKYYTPQGKAIQDTGVVPSVPFVESEPFTEIEDEETAERPEPAPEQQKPEEDMLL
                     KKAIEVLTQGKPQQALVRGAASARQ"
     gene            19024..19305
                     /locus_tag="VLE22_11680"
     CDS             19024..19305
                     /locus_tag="VLE22_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HSB15111.1"
                     /translation="MPLYEYRCTECGDVFEVIQKLSDAPLTVHKKCGGTVERLVSPSA
                     LQFKGSGWYVTDYARGSSSGSPSGKDGKDASKSTPKSEPAPAKASTSEK"
     gene            complement(19280..20125)
                     /locus_tag="VLE22_11685"
     CDS             complement(19280..20125)
                     /locus_tag="VLE22_11685"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSB15112.1"
                     /translation="MLRRSEWVLVTYFAYATVLAHLLPVPEGVAKVTLLVNVAVVAGL
                     FLLAWVDSLRRRRFLGTVRDWFPPPLLLLAYREMGWFAPATHSFRLERAWVIWDRLFL
                     NDLGARAVIESLGPVLPSVLEISYSLVYAIPYFSLALLMATGRRDRIDRFLFPFALAV
                     LCAYALFPYFPSEPPRTVFPGDNLPTWNTVFRRFNLGLLGGYGIHTSVFPSAHVSGAF
                     SAALAMRQVLPERPWAGRFLLVLAVSIATATVYGRYHYLVDALAGLGIALVAIVTATS
                     RLSKP"
     gene            20202..21545
                     /locus_tag="VLE22_11690"
     CDS             20202..21545
                     /locus_tag="VLE22_11690"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15113.1"
                     /translation="MRATRRQFLQGAAAAGVAVERAAAGQADQVSPNDKIRFATIGIG
                     GMGSGDTRTALSIPGNELVAVCDVYDGRLTRAREVFGNNLFTTRDHREILSRKDVDAV
                     IIATPDHWHAQIAIDALNAGKDVYLQKPMVQKVEDGRPLIEAEKKSGRILQVGSQYVS
                     SIIYAKTRELIQAGVIGELNMVEAWLDRNTATGAWQYSIPPDASPENIDWDRFLGNAP
                     KHPFEPIRLFRWRNYRDYGTAVAGDLFVHLLSGLHVATGSLGPNRVFATGGLRYWMDG
                     RDVPDVMLALLDYPAGERHPAFNLALRVNFKCGLPDERFGFRFVGSEGTITAGSSVVV
                     SKTQPESEPGYTIGTFPKAVQEEFLKEYRKKYPQKPLTAESLSPDQELTYAPPPGYNA
                     HLEHHKVFWSSVRSRKPVVEDTLFGFRAAGPALLSNVSYFEERVCKWDPQTLEAS"
     gene            21565..22026
                     /locus_tag="VLE22_11695"
     CDS             21565..22026
                     /locus_tag="VLE22_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HSB15114.1"
                     /translation="MALSSKVIVARRLDGQRGLFVAHSVEPDEILIKYDGPIIDHPTR
                     LSIQIDDDKHIEGTEDSNAFLNHSCEASAYVDWDAVCLRAVKAIPAGAEITCNYLTTD
                     YELHFPFHCRCGSPRCKGTIRGFKHLTPAEQLELEPWLPPFLKRKLQRCRP"
     gene            22113..22541
                     /locus_tag="VLE22_11700"
     CDS             22113..22541
                     /locus_tag="VLE22_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HSB15115.1"
                     /translation="MHPERRSIVGWLLGGGFTASILSFLYPAIRFMNPPSIPEAAVNE
                     ASGGKVQDLKPNSGKIVKFGSRPVLLIRVSDTEWKAFSAICTHLNCTVQFQQSSHQIW
                     CACHNGFYNLNGQVVSGPPPRPLDEYTVHIRGEEVVISRT"
     gene            22559..23653
                     /locus_tag="VLE22_11705"
     CDS             22559..23653
                     /locus_tag="VLE22_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HSB15116.1"
                     /translation="MTFSDKTKRASRWFEERLGLGGVREFIAHKAVPVHSATMWYYFG
                     GITLFLFVIQVLTGILLLLYYRPTTSEAYESVQFIVTRVQFGWLVRSIHSWSANLMIL
                     AAFIHMFSVVFLRSYRRPREVTWLSGITLLGLSLAFGFSGYLLPWNTISYFATKVGTD
                     MAASVPVIGPPLARFLRGGDDVGGATLTRFFGFHVAVLPGVTTLVLMLHLLLVQKFGI
                     SSPPKTEEEYRRRGDRIPQMQFFPNFFLRELMAWYAALGVLGSLAAIFPWELGAKADP
                     FAAAPAGIRPEWYFLAPFYTLKLLPSHIWIIEGELLGLIGFGLLAVFWVALPFWATNR
                     EGMERTKLVTGAGVALLAYLATFSALGYWR"
     gene            23650..24897
                     /locus_tag="VLE22_11710"
     CDS             23650..24897
                     /locus_tag="VLE22_11710"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSB15117.1"
                     /translation="MKLSLRKCWCLFLFAGLAGAAKDSCVECHSGLDGNLQKPATLFS
                     DSIHRQRGFYCTDCHGGDRTADDPEASMSRARGFIGKIPRTAVPKLCARCHSDAALMH
                     KYNPKQRIDQYAQYLTSVHGKRIAAGDAAAANCVDCHSVHDIRGVRSPLSPVHPLRLP
                     QTCAHCHADKTHMAKYQIPTSQFDEYRKSVHWNALEQRGDLSAPSCASCHGNHGATPP
                     GVNSVAAVCGTCHVLMEDLYSKSPHQPVFQSMGVAGCVVCHSNHAVLPASDKFLAGEK
                     SVCTQCHEPASAGGAAAADMAKSIERLAGALRRSDEALQRAASLGLEVSEAQLQQQEG
                     REALVKARVAVHAFDPAAVNEPVSQGLKVAEATYRSGVDALAEGNRRRVGLGISLITI
                     VITMAGLWLAIRRIEKPSRQGGL"
     gene            24901..26010
                     /locus_tag="VLE22_11715"
     CDS             24901..26010
                     /locus_tag="VLE22_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HSB15118.1"
                     /translation="MRLASVVCVFLTAGATSAADLKPLSSAEFCGRCHRAIVDAWKTS
                     VHAKAMESPLFQDALELTETRFGSSARTVCLECHSPIAVLTNDTSLRQKVSWEGVTCD
                     YCHSLRDVSFSGKNPKAKVEFSLVKSGPIKDAEAPVHGTVFSPVHLSSAACAPCHEYR
                     NPQGFPVLTTYSEWKNSRYAKSEKQCQSCHMYRVKGDVVDPKVHKSATAKVNLHQMPG
                     SHSLEQLNSTIKARLSTSREGDSLKLTVVVSNVAAGHYVPTGSPMRQLLLDVHADSYD
                     GKHFQEERRYARTVADQQGKTVDLEPLAFMSGARVVSDTRLAPDETRTETFSFPVPSG
                     VKAQVRVELRYYYSPLATTEAQKQLTFLTLRRLVP"
     gene            complement(26029..26868)
                     /gene="larE"
                     /locus_tag="VLE22_11720"
     CDS             complement(26029..26868)
                     /gene="larE"
                     /locus_tag="VLE22_11720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HSB15119.1"
                     /translation="MLDPAALQEKERKLFGILKSLGKVIVAYSGGTDSAYLAWAAGVA
                     LGENAAAITADSASIPESHKRDAEELARQCGFRHEYIDTREFDNPDYVANNPDRCFHC
                     KDELFSRLEEVGKQRGVEHIIYGVNVDDLGDYRPGQNAAKKHEVKAPLVEAGLSKAEI
                     RELSRLAGLPTWNRPASACLSSRIPYGTPVTIENVKTVEKGEDQIRALGFRQFRVRFH
                     GELVRLEIAKDEMEKALSPEMAGCFVEIFKPLGFHYVTLDLEGYRQGSLNETLGLKKQ
                     AGS"
     gene            complement(26930..29476)
                     /gene="gyrA"
                     /locus_tag="VLE22_11725"
     CDS             complement(26930..29476)
                     /gene="gyrA"
                     /locus_tag="VLE22_11725"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HSB15120.1"
                     /translation="MADREQPGAPPTPPPPPSPPPTQMPLGGDGSQNLVPINIEDEMR
                     RSYLDYAMSVIIGRALPDIRDGLKPVHRRILFGMHEMGLTANRPTRKCAKIVGEVLGK
                     YHPHGDTALYDALVRMAQPFSMRYPLIDGQGNFGSVDGDPPAAMRYTEARLSRIAATL
                     LEDIDKETVDFRPNYDESEEEPEVLPTRIPNLLVNGSSGIAVGMATNIPPHNLSEVID
                     ATVALIQNPHIPLAKIIEMVPGPDFPTGGFILGRQGILDAYTKGRGQLKLRAKAGIER
                     TGRDRECIVVTEIPYQVNKARLIEHAANLVNDKKLEGISEIRDESDREGMRIVFELKR
                     GEQSEVVLNNLYKQTQLQIGFGIIMLSIVNGQPRELGLVDTIRRFVDHRTDVVRRRTE
                     YLLRKAREREHILLGFKKALDNLDAVINLIRASRTPKEAREGLVGRFEFTEKQAQAII
                     ELQLQRLTGMEQQKILDELVEIQKRIAEYEEILGSDKVLRGVLVKELREVQKEFGDDR
                     RTRIVEDPGEIRLEDLVAMEDVAVTVTRGGYLKRTPVDTYRRQSRGGKGRIGMGTRAE
                     DVVEYLAIANTHSYLLVFTSRGRVYWLKVWDIPDAGTAGKGKQINNLVNLQPDEVVKA
                     FLPVKEFVADQYIVMVTRDGVIKKSELTEFDNPMSRGIIAVSLDEGDELIAARLSNGK
                     NYMFLASHQGKAIRFPEGEVRPMGRQARGVRAMDLAEGDFLVAAEVVEENGLMLSIAE
                     NGYGKRTPLKHYRLTRRGGKGVINMKVTPRTGKVVGVLAVADETDVMIITQDGKLIRI
                     EAAAIRQSGRSAQGVRLVKMETSDRVAAACVVTEDEANGKNGNEPTLPLQ"
     gene            complement(29611..30396)
                     /locus_tag="VLE22_11730"
     CDS             complement(29611..30396)
                     /locus_tag="VLE22_11730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB15121.1"
                     /translation="MSCFRADALSGQHIVISGGCGALGVPVIRALVEHGARMSVNDLL
                     AESEAESRLSAAAIPAGSVRYFRGDIAEADTAERFVVYAREAFGPIHTALCHAGMVVP
                     GSLVDFSEKDWDRTMSVNVKSAFLLARAAARTMIEDGIAGHLIFTTSWVSDVPWPDIG
                     AYNASKAAMKQLMRSFARELAPRKIRANAVAPGIVGAGMARRQWDADAEYQARAQKAI
                     PLGELQSPESVADAFLFLSSAAAAYMTGSVLLVDGGCSLYPMD"
     gene            complement(30423..32534)
                     /locus_tag="VLE22_11735"
     CDS             complement(30423..32534)
                     /locus_tag="VLE22_11735"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HSB15122.1"
                     /translation="MKPLILLVLSSVIMMAEGPLRGFPPEEWPARRDLESRLRAVPSS
                     ERLREYVRRMSAEPHHAGSPGSKAVAEYALGLMKSWGLDARIEVFEALLPYPVKRSLE
                     MVEPERYTAKLAEPAISEDPDSGDAGQLPTYNAYSGSGVVTAPLVYVNYGIPEDYEYL
                     KQLGIDVRGKIVMARYGGSWRGTKPKVAAEHGAVGCLIYSDARDDGFYQGDVYPKGAF
                     RPPQGAQRGSVMDMPLYVGDPLSPGWASEPGSRRLSPDEAKTLMKIPVLPISYADAEP
                     LLRNLGGPVAPENWRGALPLTYHLGPGPAAARLEVRTDMETRPVYDVLATIAGSEFPD
                     EWVIYGNHHDAWVNGADDPASGAAALLETARALAELVKGGWKPKRTLVLALWDAEEFG
                     LIGSTEWVEKHRDEIEAKAVAYLNTDSTGKGRLTGGASPTLDTFIREVARDVPDPVAK
                     TSLLEAATPKPSKDKPEEKPEFRLGSLGAGSDYVAFVHHAGVASVNLTISGDSGGGIY
                     HSIYDSFAWYSRFSDGQFVYGKAMAQVMATAVARLADAPVLPFEFDTLARAVRSYVLE
                     IDKVAGKKSGRVSFDAVLGELKRVEKSAQRYEKQLAKAMKRAQGAPPEKLKQVNQALF
                     RSERALLAKDGLPGREWYKNRLYAPGLYTGYSAKPLPAVREAVEAGRFDDADRYAAGV
                     ADALRTLDSQIRTATERLKDL"
     gene            complement(32556..34478)
                     /locus_tag="VLE22_11740"
     CDS             complement(32556..34478)
                     /locus_tag="VLE22_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HSB15123.1"
                     /translation="MVPKTAFAILAGFALPWVMPAQRLDPVKWSMSVEAAKAPPGSSV
                     VARLAAKIDSGWHLYSLSTPKGPIPTTIRIADNPAVTAYRIHQPKPTVKFDPNFKLDT
                     ELYDNEAIFLIEVQVGKEVPAGSVELTAQVRYQACDAKQCLIPVRKTASATLTIDPVA
                     PAPKATMPAGYTLFEPGKPAATAPVSDETKPISTMGGVQDQGLLGFALVAFGFGLAAV
                     FTPCVFPMIPITVSFFLNRQSGSKGQSVFQATVFCLGIIVLFSGLGLLTTAILGPFGT
                     QQLGSNPWVNGFISLVFLVFGLSLLGAFEITLPSGLLTRLDRASQGGGVVGTLIMGLT
                     FSLTAFACVGPFVGTLLAGSVSGGGTRPLVGMTSFAAGLAAPFFLLALFPAYLKRLPK
                     SGGWMSRVKVVLGFVILAAMLKYVSNIDVVMQWNFLTRERFLAAWVVLFVLPGLYLLG
                     FLRMEGIARDDKVGIWRLCIGTIFLVFAVSLIPGMFGGRLGELDAYVPPPIGGTTLGP
                     SSGTAGVSGPVWMKNQYQQALAKAREEGKPVLLSFTGYACTNCHWMKANMFPRTQIAE
                     ALSNFVLVELYTDGTDEASEANQKVLSDKFSTSAIPFYAIVGADGKVIASFAGLTRST
                     EEFLRFLRAGQNGARS"
     gene            complement(34485..35513)
                     /gene="plsX"
                     /locus_tag="VLE22_11745"
     CDS             complement(34485..35513)
                     /gene="plsX"
                     /locus_tag="VLE22_11745"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HSB15124.1"
                     /translation="MVTIAVDAMGGDHAPKTEIEGAIRAARTLGVRVMLVGREDLLRR
                     ELAVHDGYRSLPVEIVHASEQVTMEDSATKAVRGKRDSSIRVACRLVKEGVAQGVVSA
                     GNTGAVMITAKMVQGMVPGVDRPALATVFPTMNGTPAVMVDVGANVDCTPRMLAQFSI
                     MGDIYARLIIRIPNPRVGLLSIGEEEHKGNELTRGAFQLLKTLPLNFIGNVEGRDVYS
                     GSADVIVCDGFIGNVALKVSEGLVDIIKQMLQESLEATITGKIGYVLSRAAFKDFKKR
                     LDYSEYGGAPLLGVKGVCIICHGRSNTNAIKNAIRVAAEFAGGNLNSRIESELLKSPT
                     VAAAVKAV"
     gene            complement(35787..36305)
                     /locus_tag="VLE22_11750"
     CDS             complement(35787..36305)
                     /locus_tag="VLE22_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HSB15125.1"
                     /translation="MFLSVHEMELRKLRFEEAFPPGEIQFLEGKIRQASVLETSGTAE
                     LLPNTNGEIRIRGRLKVRMEADCDRCLETASFPIEVSFDLFYEPAAELPDKDELELGA
                     GESEIDFYEGEGLELEHILREQILLALPMQRTCRVDCKGICPFCGQNRNLADCGCRAE
                     PLDDRWAALKNL"
     gene            complement(36389..37168)
                     /locus_tag="VLE22_11755"
     CDS             complement(36389..37168)
                     /locus_tag="VLE22_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HSB15126.1"
                     /translation="MRVSSLRGELLAEFLGTFVLILFGAGVGAMTTLFSTGVPGEVVR
                     GGWTNITLGWGLGVTMGVYVAGRISGAHLNPAVTVAFAAFWGFPWRKVLPYSLAQTAG
                     AFLAAATVFLNYRPAFEKADPTLERTAGIFTTFPAFPQLPMAGLFDQILGTALLLLLV
                     MAIVDERNQPVPGGLAPWAIGLVVVAIGCSFGSLHGYAINPARDLGPRLFVTLAGFRN
                     NGLTDGSYVFWVPIVGPLAGGLLGAWIYEAGVRRFLPPAEP"
     gene            complement(37186..37572)
                     /locus_tag="VLE22_11760"
     CDS             complement(37186..37572)
                     /locus_tag="VLE22_11760"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HSB15127.1"
                     /translation="MLKRRALLAAPALFCSGSAVLSAADGEMLVHNVYFSLKDASDAA
                     KADLVAACHKYLKDHEGVVFFAAGSRAVDFKREANDLGFDVALNVVFKAKADHDRYQV
                     HEKHKQFIQENVANWSQVRVFDSYVS"
     gene            complement(37663..38136)
                     /locus_tag="VLE22_11765"
     CDS             complement(37663..38136)
                     /locus_tag="VLE22_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15128.1"
                     /translation="MRLQSFRAAVLLAAFSVGVCSAQIADLSGTWRLNVQKSRWGNLQ
                     KPFSILVTVEHKEPLLKYSGTMVYSGGEDTRTFAFSGAIDGKEYPITRSFGKGKVVIQ
                     RINNWTTESVFRSDDGQWSETTRTSLSRDGKQMRRTIRRKGPEGELNWTEVYERS"
     gene            38246..39346
                     /locus_tag="VLE22_11770"
     CDS             38246..39346
                     /locus_tag="VLE22_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15129.1"
                     /translation="MQDLNLEHVPPPPPRKDYRIGVIGAGFIVRDVQLVAYANARYNV
                     RAIASANPEQARAVAAHHGIPKVYDTWRELAADPSIEVVDLAVPPGEQPAILKELPRI
                     GPQLKGVLAQKPLALNYAQSKEAVQAFDGSGITLSVNQNMRYDQSIRALKSLLTRGRL
                     GEPVLATIEMRAIPHWQPWSRKYGRLTLLIMSIHHLDAFRYLFGDPESVYVSVRTDPR
                     TKFPHRDGIPLYILEYASGLRASAWDDVWAGPAREGAASDIYIKWRVEGTEGMAWGTI
                     GWPSYPNATPSTIHFTTTREPGVWFSPQWKEVWFPDAFEGTMGQLLTAIATGAEPAVT
                     ARDNLRTMALVEACYRSIDEHRPVSLKEIMSE"
     gene            39343..40167
                     /locus_tag="VLE22_11775"
     CDS             39343..40167
                     /locus_tag="VLE22_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB15130.1"
                     /translation="MNLGLINSAWLGSGIGSAEGIRKTKEIGFDTIDIFADPLEIDAR
                     ERKLIKETCREVGLPITSVCAVALGIADFNTSVRRFHAERLRRYADLVYEFDGKNLLT
                     VVGEYVWQQEVIKPEDQWGWAVEEVRKLGEYAGSLGIEIAIELEPFHLSIVNSVDRMA
                     AFLDDVGHLAVKANLDVSHLSLVRTAPEEIQKLKGRVAHIHFSDCDGKVHGDLPPGRG
                     VVDFPPYLREIANLGFDGTLSIELEYSPEPGRIMEWVTEAYRETARLMKEAGLHRP"
     gene            40234..41580
                     /locus_tag="VLE22_11780"
     CDS             40234..41580
                     /locus_tag="VLE22_11780"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HSB15131.1"
                     /translation="MSEWSPASWREKPALQQPVYPCAGELESILRELSNLPPLVTQWE
                     VDALKAQLADAALGRRFLLQGGDCAESFNDCNAGTIADKLKILLQMSLVLVHGSNRPA
                     IRVGRFAGQYAKPRSEELETRDGVSLPSYRGDLVNRPGFTAADRTPDPRFLLRAHGRA
                     ALTLNFIRSLVKGGFADLHHPEYWDLGWVRHSPLASEYRRIVESISGALQFLENVLGI
                     RAGEIERVDFFTSHEGLHLPYEEAQTSRSDAGGRWYNLSTHFPWMGVRTSDPDGAHVE
                     YFRGIANPIGLKVGPSTSPATLVHLLDILNPADEPGRLTLIHRFGCDRIATCLPPLIE
                     AVKARGRTVLWCSDPMHGNTLQTADRVKTRRFADILSELEQSFDIHSALGSHLGGVHF
                     ELTGENVTECIGGARGLTEEDLGRAYKSEVDPRLNYEQALEMAMLIARKMKTLGGR"
     gene            complement(41587..>42479)
                     /locus_tag="VLE22_11785"
     CDS             complement(41587..>42479)
                     /locus_tag="VLE22_11785"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HSB15132.1"
                     /translation="TPRDMRKTTLLKKYIHDPEILVIPGVPDPLCARIASKVGFKAVF
                     VSGCASSAATLGAPDVGLMTMTEMVECAARIVDAVDIPVFADGDTGHGNVTNLIRSMR
                     QFEKAGVAAIFFEDQVAPKRCGHMSGKQVVPIEEMLAKIKAAVDTRIDADLMVMARTD
                     ALAMHGIDEAIARMQRYIEAGADMAFIEAPQSIEQMRQITSAIKAPNMANMVPGGKTP
                     ILPAKQLEEIGFAVVAHPTALTYAMAKTAQDVFRHIRDTGTSSGLEGRMVAFEEFNDL
                     VGLPEIRAKEREYLSGLAEG"
BASE COUNT         8124 a        13726 c        12485 g         8144 t
ORIGIN      
        1 actgatcgcc gagtgcgacc ccgcgcgggt cgacgaggtt cgttgccagt ggcccttcct
       61 ccgggaccgc cgcatcgacg cctacgcacc gattctcaac cgctggctgg accgttgagc
      121 cgcacaaaaa gggtcgcggg aggacggggg cctcctcccg cgacgaagga aatcgcgtct
      181 gtcaggaccc gctgttcatc cccaggtcct tcccgagttg catctccgtc tggcgggcac
      241 tggcgtaagt ctcgaactcg tgtacggacg ttgcgatctg atccgcctcc gcgaccatat
      301 tccgggtgta cttcccgtac tccggcttcc acaggccctg ttggttagcg ttcagaaaga
      361 gaatcgcgtc ctgggcgttg tccgccatga gttgaaccag cggcgcgacg tgatcgatga
      421 cgcgctgttg ccaggggaac gccgacgatc gaatggtttc gagacggcta agcttcgtcc
      481 ccatgtcgtt gaccgcctgc ctgatctcct caagtttctg accgtggaca tcccagccaa
      541 ccgtgagatg caatcgcagc acctccaatt catctgccag gctcctgacg tgctgcgaat
      601 cggtttcgat gccactcagc agatcggtgg cctctttgtg aatgtcccag gttgaggacg
      661 ccttggccgg gcgcgcttgc acagtcgcct ttgacgtccc ggcaaaggac atcgcggata
      721 ctgcgaggag gacccccagc agatatccga ccctactcag agttcttccc ttcatgcttc
      781 ctttagctcc tttcactccg gcatcggctt tcgcctaccg gatcttcgcc ccatactgga
      841 agacgagttt caagcacagg cggattacta ctgttgggaa ggtatatcgg agcggagaat
      901 ctcgtttcga tggaaaggct accgaataat tcgtttgcgg accgcgtaca gaatcaggtc
      961 cgggattgta tgaaggccta gcttttgaag aatgtgggaa cgatgtgttt ccaccgtata
     1021 cagactgagg ttcagtaggt tggccacgtc cttgttgctt tgtccctctg ccagaagctg
     1081 aagaatctcc cgctcgcggg cggtcaacaa ctcatagctg tccacttcgc ccttccgctc
     1141 gagctgacgc acgtaatctt cggcaaggag cttgctcact gcagggctga agaaagactt
     1201 tcctaacgcc actgcgcgaa cggcgccaat gagatcgacc tcggccgagt ctttcaataa
     1261 atatgcccgg gcgccagctt tgagagctcg catcacgtaa ctctcatcga cgtgcatgct
     1321 gaggatgaca acggctgtct gtgggtgtcg gatagcgatc tggcgggtgg cctcgatgcc
     1381 gttaagtctc ggcatggcaa tgtccataac cacgacatcc ggcgcgtgga catccgcgag
     1441 ttcaactccc cggcggccgt cttcagcttc cgcgacgact tcaaaatcgg gctgccgttc
     1501 cagcaggagc cggagtcccg ctctcatcac ggtgtgatca tccgccagaa ggattcgaat
     1561 cgatgccatg ccgtcaccca tacagcccgc tggactctcc cctcaacggc aggtcaaccc
     1621 ggagatgagt cccctgtcca ggtttcgact ggatgacaaa cacaccaccc aagtggcgca
     1681 cccgctcctc catgccgacc aggcccagtc ctcgggtgtg cagggcatcg aagcctttgc
     1741 cgtcgtcctg aattgacagc agcagccggt tcttctcctg gcgcacgacg atctggaccg
     1801 actgggcgtt ggagtgcttt gatacgttgt tcaacgcttc ctgcacgact cggtacacgc
     1861 aggtcttgta gtcgtccggc agctcgtccg ccgtctcctc ggcggcaagc tcgatgatcg
     1921 ttcccgtccg ccgggaggct tcacgggtct gccagcgcaa cgcggggacc aaaccgaaat
     1981 cgtcgagcat cgatggccga agtagcagtg ccctgtttcg cacggattga aggctttgct
     2041 cggcgagctt cttgaccgta tccagcagtc tgcgcgcggg gacgttctct tccggaacag
     2101 tggccacaac attgccgagg tccaccagaa gggcgctcag agactggccg acctcatcgt
     2161 gaagctcgcg cgagatggtt cggcgttcct cctcctgagc gtccacaagc ttggccgaca
     2221 ggttctccaa ttccgagcgc gcccggactg tttcctgata tcgcagacgt gcgtcccgtt
     2281 ctaaccgcaa gatctgcatg atgctcagcc cggccacgcc gatgccgagc ccgagcgtga
     2341 tcgtcagtat ggccacgatg cgactgcgaa agctccggta gatttccgct gaacgtaggc
     2401 cacccgcttt caggacggct tcgttcatgc catccaccct gtcggctaac cgcagcatcg
     2461 cgtcccggcg aggcaaaacc agttgctcca ggaagcgata gctcagcgat cgccgttgcc
     2521 ccgcgtccca gtcgaagctc gggctgatgg tcttccaata ctcggcaagc tcggcgcgta
     2581 aagtcgcgag ttgcgggacg tcttccgaaa gtccgtctcc gcgatactcg tcgagcgccg
     2641 cattcgtttg tgctcggacc tcctgcaatt ccgccaggcg ggaccgtgcc ctgccctggt
     2701 cggggtccag gaggtagtcg cgcaccagcg ttccggacaa gtacaagctg gagcggacgc
     2761 gatcgagagc atgatgccgc gacagatagt ccctggtgac ctcaacattc tcgctctcga
     2821 tgctgtgcag gacgcgaacg gactccgctc cgatgatgcc catgagcgcg agaaccgctc
     2881 cgaatccgac aagagaaacg agcctgacgc cggaccgtga aggctgatcc tcgtccggcg
     2941 tttccttact ccctttaatc ttttccgagc tccccattcc gccgcccgga tacagttcga
     3001 gccgacaatt cgagatcagg cagacccact ccggtgcacg ccgcgcaagg cccatgcatg
     3061 actatatgcg ttctcgacaa tgtgcgcgaa ttctatcgac gagaacggcc gcgagaccaa
     3121 gtcgtaggcg ccattctcga tcgcggtgat ccagaaccgt tcatcaacga cggtcgtgac
     3181 gaccacaacc gaaactggcg gcgcgaactc ctccgagagc gcgactgcat cttgccagtt
     3241 accatcacgg aaggacgcct ccgcgagcag aaccttcgcc ccagtcaaac ccagcatcgc
     3301 gcgggcgcgt tcgatggtgt ccgcgtggtg aacccatatt ccagaccttc tcagcgccct
     3361 cgtagcctcg gcaaccgacc gggcccacgg tgtgacaaac acgcaatcga tcttcgcgcc
     3421 atacgccgag ccgccaagct cgcgcgctgg aatcggcgtt gcggctttcc tccgggtcgt
     3481 gtcaagcatt cggcttcacg ccgcgtcgtc gagtacgcgt cctcgctcgc ggtaccagcg
     3541 gccaccctgg ctgctgtcct cttcctcctg gcagtggatg cacagcgagg cccatggaac
     3601 tacgagcatt ctcccaaggc ttattgcgcc gccgcaccct tcgcaccggc cgtacgtacc
     3661 ctccgcaatc cgcttgagag ccgcgtcaac ctcgtgtgac agctccgtgc gacgttcgag
     3721 gccgccgatc gccatctccc ggtccaccgc gaactggatg gcatcgattt catccggcac
     3781 cctctccaca gcaatgtcgt cgattcgggg ggcttccaac ctcaattccc gttgcttgcc
     3841 gcgcagcatt tcgacgattc gcttcaactc gctctcgctc atcccagttc tccctgttgg
     3901 taaacagaat aagctccgcc gccgatcatg gaaataccgc ctggcgggta tagtcggcga
     3961 ccaaatacag ggatagtccg tcctgctccc aagtggaaaa atggtccgga ggcgctacta
     4021 tcgttgcgaa ggagcagaaa tgacgcgcat cccgattctg tggctggtgc tcgctctcca
     4081 ggtcctgccg cttctgtccg agaacttcac cggcatcaag cccagtcctc aacaagttgc
     4141 gtggcaggat cttgagatcg gggtactgat ccacttcggc ccgaacacct tcatggaccg
     4201 cgagtgggga gacgggaccg ctgacccgaa gatcttcaac ccgctgcaac tcgatgccga
     4261 acagtgggcg acggcggcca aggccagcgg cgcacggtat ttcgtcatgg tcgctaaaca
     4321 ccacgatggc ttttgcctct ggccgagccg ccacacggac tacagcataa agagcagtcc
     4381 ctggcgcggg ggcaagggcg acctcgtccg cgaagtcgcc gacgcctgcc acaggcaggg
     4441 gctgaagttt ggcatctacc tctcgccatg ggaccgtcac gagccgtcct acagggacaa
     4501 caaggcctac gacaggtact acgaggccca ggtgctcgaa ctggcgacag ggtacggcga
     4561 ggtggtcgaa ttctggctcg atggcgccgg cagcgagggg cacgtctacg atttcgaaag
     4621 ttacgccggt acgctccgca ccttccagcc gaacacgctg ttcttcgccg gtgtcggctt
     4681 tcttccttgg gctgatatcc gatgggtcgg taacgaggac ggctcggcat tcgaggacaa
     4741 ctggaacgtt atcgatctcc gcggccacct gcgctggcgc cccgcggagg cggacacccc
     4801 gctgcgagag gggcactggt tctggcaccc gaacgacgag aagagtctca agccactcga
     4861 caaactgctt gatgtctacc acaagagcgt cggccgggga gcccaacttg ttctcggcct
     4921 tgcaccggat aaccgggggt tgatgcccga cgccgatgtg gcccaccttc gcgaattcgg
     4981 cgacgccatc ggccgcatct actcgactga cttggcgcgt ggcaagacgg tctggacgcc
     5041 tccttccgac atgcacacga tcactggtga agtcgatttc cgagtccccg tcaggttcga
     5101 tcggaccgtt ctggctgagt ggatccatct cggtcagcac gtgcaacgct acgatattca
     5161 aaggtgggat gggagccgtt gggagaccgt gcacgaaggg acaaccatcg gtcataagaa
     5221 gatcgacatc ttcccgcgca ccaccgccat ccggatgcga gtccgcatcc ttcaggccac
     5281 ggagacgccc cggattcgcg gtttccaggt cttcgacggt gcacggtagc ggatgctaca
     5341 gttgtctgca tggagcctgg cgagcgtctg acccggcgcg acctcgttcg ggtgctcggc
     5401 gtttcgctgg gcggcttgcc tcttgcaaag gcggctggat attcgccatc gctttcggcg
     5461 ggcgcactcg ggctaccggg tttgtttccg ggaagagtga tcggggttga gcatcgtggc
     5521 tcgttgctgt ccggaaatta tcacggcccg gtgatcgaac agatgtttcg gcgcgggatg
     5581 acggagctca ccgggacacc agcgtggcag gaggcgtggc gtcttttttt ccaacccgga
     5641 gacgtagtcg ggatcaaggt gaatccagcg gggcagcccc acatcatttc ttcacccgaa
     5701 gtgctctggc agattgttca aggcctcgag gcggccggaa tcaatcggaa tgatgtcgtt
     5761 gtctacgacc gataccgcga ggagtttttg tccgccggca tggatcgctg ggttcctcag
     5821 ggcgttcgga cgatgttcgc caccgcgcat tgggacgata ttcagcagac gatcgacggg
     5881 tatgaccctg accacttcat ggagatggcc attggcatgc ccgggcagaa cgtcaacgat
     5941 tctgccgtac gccgctcgta tgccgcgcga ttcataacca aggcagtcaa caaactggta
     6001 aacctgacaa ctcttaagga ccaccaggcc gctggagtca ctctggctct gaaaaacctt
     6061 tctcacggat tcgtgaacaa cgtcaaccgc tcccaccccg actacacccg caattacacc
     6121 ggggcgttca ttccagccgc tgtgtccatt ccggcgatcc ggaacaaggc agtgttacac
     6181 atcatcgacg gcgtcaaggg cttgtatcac agcggcccac acgcacagcc gcaattcttg
     6241 tgggaaaact gcactctcta cttcgccacc gacccagtcg ccctcgatcg tgtcggctgg
     6301 actgtgatcg accagaagcg agtctccgtt ggaatgaaac ctgttcaact ggcccccgcg
     6361 gatgaatata gtcactggca aaaccgtcag ccggaacaca tcgaaatcgc gggaatgatg
     6421 gggctcggcg agtgggaccc gtcgcgcatt taccaccggc acataaggct cgacgtaccc
     6481 gcatctcctc gctcgcgcgg ggaaggccgc attgcgcttc caatgccggg ccgcgaccgc
     6541 tagcgtttcg gcggcggcgc atccgagggt ggcgtcggct tgccgtccac ggtgaccggt
     6601 tccatcagct ccgtacgagt gccgtccgga tcgaacagat tgagctgtcg cttccggttc
     6661 gtgcccacgc gtggctccaa ggcgcgcaag taacccgcgc ggggcgcccg cccccgaagc
     6721 acgccgagcg acttcgggat atccgtcgtc tccagacaaa tgtgatgcgc actgccgcgc
     6781 aggtccggct ccggaagctt ctcgtacagc atcagttcga tgtagtcgtc gccctcagga
     6841 actttcatgt tcacccagga aagcaccttc ccgttcccgc tcccccgcca cgtttcggtg
     6901 aatccgagca cgtcacggta gaacccggct gatgcctcga gatccccggc gaggatcccg
     6961 agatgcctca tccgtggaga gatgcgctca tcgccgagga actgcccttt tgttcgcatc
     7021 gccattccgt cgggttcata ctcaacgaac tccacatcgt gcccatcggg gtcgcgcacg
     7081 gagaagttgg agttgccggt gcgtcccttg ggcaccttgt ctgggacctt gaagccgcgg
     7141 gaagcgagat atgctcgcat ggcttccgcg tcatccgtgt gaagggcgat atgggacaga
     7201 cggtcttgat cgggcttcag tcccggaaac aactcgatgt actggtagtc attcaccttg
     7261 atgaatgtga acatcacgga gccatcggga ttattgatgc cgaatggctc gccgaagccg
     7321 agaaaatcct tatagaagcc ccgggacttc tcgatgtcgc tcacgtagac gccgatgtgc
     7381 gccaccccaa ggatgcgcgg gcgccgggcc tcctgtgcac cgagaaacat ggaacagaac
     7441 agaagcaatg cagttattcg catacgagca gcaccttcag aatttctttc tcctcggcgc
     7501 ggtcgaacgc gcgcggggcc tcggacagcg gaaagcagtc tgagatcatc tccgacaagc
     7561 gaagcttccg gctcttgagc agtcgaagcg ccggctcgaa gcgtccgcaa cgagaaccga
     7621 tcagcgtaat ctcgttcacc acgaccggcg cggtgtccac tgagacggtc ccatgcaccg
     7681 tcgacttcat gatcacaacg ccgcgaggcc tcgtcattcg aaccgcttcg gtcagccctt
     7741 ccggcgagcc cgttgcctcc accacccagg cgtaccgtgc cctcggcggt tttgctcctg
     7801 cccgccgagc ctccacgccc gccgcctctg cgatcttcat cttgaccgga tggcggccgt
     7861 agagatgcac cgctgcgccg tgcgcctgga gcacttgtgc gaccagcagt ccaagtttgc
     7921 cgtcgccgag caccgcgacc tcactgtccc gcgggactcg cacctggtcg aggatctcac
     7981 aagcagcggc gacgggctcc gtgaacaccg cttcctgggt ggatacggag ctgggaacca
     8041 cgcgtaggtt tccttccggc agcgtgaaga actcgctgaa agccccagga tgtctcacga
     8101 tcccgagcac agtgcggttc gggcaatgcc ggcccaaccc ccgccggcac caatcgcagc
     8161 gcccgcaagc caggttgatc tcgcccacca ctcgccgacc ggcgagcgcc gggttgtccg
     8221 tctcgatcac ctcgccgaca aattcgtgac cgggcgtccc gcgaaagtcg tagtagccgc
     8281 gcctcagttc gatgtcggta ttgcagatgc caccgcagac caggcggatc agcgcgtgtc
     8341 cttcccggcg ccgtgggcgc ggcttccgga cgacatccac cttgccgtct ttgatgtgga
     8401 ctgcgagcat ggcagaggtt cagtataacg gaggctggca cacgcctttc ggaataacta
     8461 cgtagtggta gtagtcgcct cgggtggccg gagcgcggcc agtaccagca agattgccgc
     8521 cggccacacc tgcgcaaaga tgcgccccag cgagatgctg agcctcgacg gtgtagtcag
     8581 gtacgccccc gcggaccccg cgaccaacag tgccagtgtc acctcagcca ccagcggata
     8641 cccacgccgc tccggctcgg gaatgaaccg gagagttgca gccaaaatcg ccaggagcaa
     8701 cagcggatgg gtccacgggt cgcccagcat cagcaattcc gaaagcaaag cacgggctat
     8761 cgtgaaatag cggtctgcct cggcgagcct gctcattgct tcgaagactg actgcttcgt
     8821 cagcggattg acgttcgggg caagcgcgag tttgaaccac agcgttacaa gcaaacccgg
     8881 cagcgaaccg aagatgaacc atgctagcaa ccgtgccgtg gttctcacgc ggcggctccg
     8941 gggttgggcg atgcatattc ccgccaccga acacgcaagg aagagcagcc cttcgttctt
     9001 cgtccacgcc gctaggctgg ccgtcgctcc cgcgagcgcc agcacgttgg cacggcactc
     9061 tgccgcttcg tgcgtcagac tcaacaggac caccgtgccc aaaatgtaga agcccattgg
     9121 aacatcggca tactgcgatg tcccttgtga gagaaacccg gtgcttgcca gcagtatgca
     9181 tccagcgagc agacctaggc tcgtggatcg taacaccgca aatccggaga ccagtaccgc
     9241 aaaagtgccc gcaaaaaaca gaaacgccgc ggctgcgggc acggcgggtg gtgtttcctt
     9301 acctccgtac ctccacgcgc gagcaacgaa acccggaagg agcagcgggt aatcgggatg
     9361 agtcttagcc agcagtggac tccacgcgtt tctccactcc tcgccgtccg cggcgagaaa
     9421 cttcgccctt acgttccaag tactccacgc gtcccaatcg ccgtggatcc tcgcctcagt
     9481 attgctgagg aaggcgagga ctaccagcag aaatccaaca gtcagcgctc ctgcgagcgc
     9541 ggcgttccac cgaaatcctg gcttctcggg agattccact ggccgcgctg tgtcccggaa
     9601 aaccagccac accgcgcctc ctgccagaaa caacataagg tcggccgcaa agagaagccc
     9661 cctccccgct aagccggcag atcggaccag gaagtaaagg actgaggtgg ctcctgctcc
     9721 aataccggcg ccgagcgcga tcgtgagaac acgtgccgcc cagcggggcc gcagggagac
     9781 tggccgcagc agcgtgtcta ccaccagcca gcctgtggca atggaaacga gaaaggcgac
     9841 cgcggccatc atcctaacgg ccgatcctcc ggaatatcag caccccgctg ccgagatccc
     9901 gtgcgagctt cagttgatgg cgtcgctcga cggcgcttac ttcggccggc ttcgagaagt
     9961 tcccgagcac ccagtcggtc gactcgcggg ccggcaactc tacgagaatc cggggcgcaa
    10021 gtgcgtactg tgcgccgaag aacgccgcgc ttccgcggac ttgttcgaaa ggcacgtcgg
    10081 acacatagcc gaccagcacg tctcgcggaa ggatcgttgc cgcttcgcgc aatcgctgga
    10141 cctgtatctc caccgcgtac ggatcgccgg ctgtgcggtt tgtgagcgtc gtcgtcctgt
    10201 agtactctag cgacgccgca agcgacagca ccgcgaccag tcctacgcca gagaggacgc
    10261 gcacagggta cctggacttc gttgtagttt caggagcgcc catggaacag atcgatgaga
    10321 atagcagaac cggcggcgtc gacgcgcacc cgcccgttat aatcaaggct tccatgcaag
    10381 ctgaactggt ctgttttagc gccggctgcc gtgcgcgctt accactcaca gaggtaatct
    10441 acaactgccc gcgatgcggc ggcctcctcg aatgcgacta tcccggccgc gccgttgaca
    10501 cgctcgcgct aaagcgtacg tggcgagaac ggcggatgag caacgctccg ctcgaccgca
    10561 gcggggtttg gcggtaccgc gagatgatcc cgtttctcga gtcgtacgac aaggtcgtca
    10621 cgttaagcga gggcaacaca cccgtcctca acgcacccca cgcggccgcc tgggtcggac
    10681 ttgagcagat cgccttcaag caccaaggtt tcaacccgac gggttcgttc aaggacaacg
    10741 ggatgacctg cggcgtggcg caggctctcc gactcggaat gaaacgtgtt gcctgtgttt
    10801 ccaccggcaa cacgtccgcg tccatggccg cgtatgcgag cgcggcggga ttgcagccgt
    10861 tcatcttcat cccgcacggc aacatctcgt acggaaagct tgcgcaggcg ctcgaatatg
    10921 gcgccaaaac cttgcaagta gaggcgaact tcgatcaaat tcttgccctc gtgcgggttc
    10981 tcgccgagcg gctcggcatc tacctgctca actccatcaa cccgttccgc atcgaagggc
    11041 agaagacgat catggttgag ctgatggacc agcgggactg gaacccgccc gactggattg
    11101 tcctccccgg cggcaactta ggcaacactt cggcgttcgg caaagcgttg cgtgagatgc
    11161 tggaccttgg cctcatatcg cgaagccccc gcctggcggt cattcaagcc gaaggagccg
    11221 cgccgttcta cgggttgatg cactcgtccg atcgctcaga ccttccgaag gtcgagaacc
    11281 cggagaccct cgcgaccgcc attcgtatcg gcgatcctgt ttcctggcca aaggccctgc
    11341 acgaggtgga gacctcgggc ggcatcgtag aaaaagtgac cgaacaggag attgccgacg
    11401 ctaaggcgca gatcggactc tgcggcatcg gctgcgaacc tgcctccgcg gcgacccttg
    11461 ccggcatccg aaagcttcgc gcggccggcg tcatcgcgcg gaatgtggac gtggtcgcgg
    11521 tgctgaccgg caatctcctc aaagaccccg actacatcta ccgctatcac acgggtcagc
    11581 ttgtggggcc gagcggccaa aaactggaat ccaccttcgg taatcagccg gttgtcgtcc
    11641 cgaatgaccc ggaccgtatc gcggagatgc tggcgtagga acgcgaaaga aacctaccct
    11701 accgaggtgt gatcagcccg cccagtttgt cctgctccgc cgcagccgaa cgcgggaagc
    11761 gggttgagta cgcttcgaga tatttgaggc ccgcgcccgt gttatacacg agaatcttct
    11821 cgtttggctt caccgcgcct gccttgatca gcttctcgag cgccgcaatg cacgctgcgc
    11881 cttcgggggc catgaacaat ccctcctccg cagcgaaccg tatcccctcg tcgagcagtt
    11941 cggcgtcggt gacggaaaca gccgtaccgc cgcttgcgcg cagaatgtcg agaattagaa
    12001 agtcgccgag cggcttcggc acacgcaggc cgctggctgc tgtacaggcg ttctcccaaa
    12061 agcggcaacg cgattcgccc cgctcgaacg cttcgacaac gggacggcaa ccctcggcct
    12121 gaaccgcgac catcctcggc cgcttgccgg agacccagcc gaacgcttcc atctcctcga
    12181 acgccttcca cattccgagc agcccgactc caccgcccgt cgggtacaga atgacgtccg
    12241 ggagttccca gtgaaactgc tcggcgattt cgtagcccat cgtcttcttg ccctcgacgc
    12301 gatagggctc ccggagggtc gacatgtcga accagccctc ggtggaacga ccttcggcga
    12361 ccaaacgggc gcagtcgctg atgaggccgt ccacgagcgt cacgtgcgcg ccgaacgcca
    12421 tgcactccac gtagttggac tgcgggacgt cgcgcggcat gaagatgtgg gcctcaatcc
    12481 ccgccgccgc cccgtaggcc gccagggcgc tcgcggcgtt tcccgccgat ggaatcgcca
    12541 gtttgcggat gccgagctcg acagccatcg aaaccgcgca cgatagcccg cgcgccttga
    12601 atgaccccgt cgggttcacc ccgtcgtcct tcacccacag atcttcaagc cccaggcgcg
    12661 ccccggcatt tctacaccgg accagcgggg acatgccctc gccgagcgat acgatcgaac
    12721 cgggcttccc aaccggcagc acggcggcat agcgccacat cgtgggtggt gcgttgttga
    12781 gccattcccg gctccagcct tgccgcacct gcacaagatc gtagcggacc gaaagaggcc
    12841 cgccgcactc gcacacgttg tgaacgcgtc cagcctcaag ctgcttaccg cacaggctgc
    12901 attcgagatg cgtgattgtc gtcatgtatt tcctgccgct tctcctatca ctgctcgtag
    12961 ggaatccaaa cctgcatcgc cgatggcccc cggttcgaga acgcgtaata cgggataaaa
    13021 gaaacggtca ccggcctccc caccctgctg cgcacgtccc gcagtgcctc atagagccac
    13081 cgctcggtga gcggtggctc ctccgctacg cctgcgtgcc ataactccac aatccccccg
    13141 agcttgtcgc gccggtattc gccggtgaac cccttggctc gcccgccctc gcgcaagagc
    13201 ttcacgtcaa agatcgacgc tacgccatac tgatcggcct gctccatgca ataaaccaga
    13261 ggtccgcgct ccaccgccac acggccagcg ttctcccgta ccaagggatt cgcggacgtc
    13321 agccgcgggg cgatcccgag atccagcacc actcgatcgt tcggttgcca cttccgccgg
    13381 attgcgagat atgtgccggc cttcatcgga tcgcgcaccg gccggccatt taccgctgcc
    13441 cgcgcggggc ttgcccagcc ggggacgcgc agaaacagcg tgaactccac tgcgcgctcc
    13501 agccgcatcg caatctcgac tttgccctcc cacggatact tcgtagtctg cttcaatcgc
    13561 aatccggttc cgtcctccag ccgccaatcg agcgtgttgt tgtcgtagag atgaatgtgg
    13621 agaccctcgc ggctggtgct gtagaagtag ccaggcagcg acgccagtac ccgctggaga
    13681 ttcggaggac agcaggtggt actgtaccag gggttgcgga ttttgtcctc cgggtcgccg
    13741 gtgagctcga gcggattccg atagcagtag agtgtaccgt ccagcgacat gccggagttg
    13801 actccgttgt acagcgctcg ctcgatctca tcggcgaatc gcgcctcgcc ggtcgccgcc
    13861 agcagccgcc agttccagat catgttgccg atcgccgcac agctttccgt gtacgcgagt
    13921 ctgttcggca gctcgtacgg ctcgccgaac gcttcgccct gcgcccggga accgacgccg
    13981 ccggtgatgt acatcttgcc gcgcgtcatg tcctgccaca ggcggtccag cgtccgccgg
    14041 tacgcctcgt ccccggtctc gatgaagtag tccgtcgccc cgctcacagc gtacatggcc
    14101 cgcacagcgt ggccttcgag cttcgtccgc tcggtgaacg gcttgccgct gaacaggtac
    14161 acaatgtccc ggcgactcag cttcaggcgc tcgccgtccc cccgtacgag atacccggcg
    14221 aggtccagat gccggcggtc gccggttgtc cggtacaact ccaccagcgc cagctccagc
    14281 tccggatggc cagtgagcag cggcctctta ttcggcccga aatcccgaat gagatattcg
    14341 acgaaccgca gcgccccgtc cagaagcctg cgattccccg tagcccggtg atacgcgacc
    14401 gccgcctgca acaggtgacc gaggcaatac agttcatgac cgctctgcat ctgctggaac
    14461 cgcagcggca cgcggtcttc gacgaagtac gtgttcaggt aaccgcttgc ctcctgcgcc
    14521 gccaggatct cgtccgtcag ttgatcgaac tgcgcgcgaa gcttcggatt gtcgaccgat
    14581 tgcagcacga acgccaccgc ctccatccac ttgtagaggt ccgaatccgt gtacagcggt
    14641 ccgcgccggg tgacgttctt acgtcccgaa agccgccgga aattgtccac cactccgttc
    14701 tcttcgagca gcgcgagcag actggggatg ctcctctcga cgttcacctg acgccgcctc
    14761 gaccagaatc cgtcctcgat ggtcacggct cgcaccggca cgctgtgcag tcgcgcgtgc
    14821 ggcgacttgc ggaggtccaa cacgccggcg tcgcgccagt cttgtgcaat cagcaggggc
    14881 gccagcacaa gcagagcaag aatggtgcgc ggaaacatgg tgtcagcact ccattatttg
    14941 cccgaggcga ccctcgggaa cagcgtcagc cgcagattcg tgctgccata tgggatcagc
    15001 gtcaccgttt cttcggtttg ggcggaacgc gctgggcttt cgggcatggg tccggcagaa
    15061 ccggctatta gcttccattc cggaatccgg cggcctttcg cagaaagcac caccggcgcg
    15121 ccgtccggcg acaggggcag agggcccacc ggccgttcct ccatcgagat cgaggcggcg
    15181 gggttctcag ggtccacggc cagcccgtaa ttccacgcag tcgtcgggtg gacctcccag
    15241 tcggcgtgcg gctcctggcc tttgaccttc cgccattcct cgccgatctt cagcgagaac
    15301 accaatggtc ctctcagcac tgcgacggta ccccgatatc gtgtctcggt gcgcactggc
    15361 atcggaaaaa ggatctcaac cgtgtcgcca gcctgccagc gccgctcgat ggtgtggaag
    15421 gtacccggct tcacgccgtt ggtgcgcttt cccgcgacag tgattgcggc gtccttcgcc
    15481 cagcccggga tgcgtagcgt cagcgggaac gcgaccggct tcgcgggacg gaccgtcagg
    15541 cggatcgcct cgcggaacgg atactccgtg tcctcctcga tcaccaccgc gactccgccc
    15601 cgcacacgcg cctccacacg cgacggcgca taggccaccg cggccagccc ttccttgcgc
    15661 gtcgccatcc aaaggtgtgc ggcgaatttc ggccacccct ggtgcatatt cgacgtacag
    15721 caaccgaaat tcggctccag tccgtagatg ttcgcgtccg gcccgttgtt ggtccagttt
    15781 cgcttcttat cgatgctgca catcacctgg ttcacctgct ggtcgtactg gtgggcccac
    15841 atatccggct tgaaggtcgc cggcagcgcg ttgaaggcga tgcgttccag ccggtctcca
    15901 aacgccgggt cgccgaggat tgccagcagt tgttcaagcg agaacatccc ctcgaccacg
    15961 gcgcacaact ccgttccctg cgtcgggtcg agccctgcat agtgctcgtc gccgctgtgg
    16021 acgccgttgg cctgcccatg actccggtcc atatgatcga gcagtgtgta gatcgcgttc
    16081 cggtctgtct cgtcgcccga aatttgccac cagaccgcgg acgttttcaa cgccattgca
    16141 ttgttgacaa cgtgcgtctt gaggctgatc tgtcccttag tgaccttcgc cggataccgg
    16201 aagtcggcaa agtgcgtttt ccagtcgaag gcctgtggac ggagtgcgcg cgcaagctcc
    16261 agcagttcct tgtcgccagt gcggttgtac aaccagatga tgctgagaat ctcttccgcc
    16321 caccggtacg atgcccactg aaccagcggt ctctccgcag catgttccaa gtgataccgg
    16381 aggtacttat gcatcagggg gacgacccgc gggtcgcccg tggcctcctg gtactgcgtg
    16441 agcaccttca gcatcaccat gttcggccac cagtccgtcg cctgccactc gcgcgcgtac
    16501 tttccctttc ggggatcggg accgatcgcg ccggtctcac gctgattctc caaggtccag
    16561 tcgacccact tcttagcttt tgcgatcagc ctctggtcct cgagcaggta cgccagtgga
    16621 accagaccgt ccagaaaata agggccccgt tcccaactct cgccggagcc gccgagccaa
    16681 ccgctgttcg ggccgagatc gggccagaac tcgtcgaggt gcccgctcag tccgttagcc
    16741 tggatctgga gctgctgccg cagccatcct tgcggcttga cggagcccag cggcagcggc
    16801 gccatcgcgt ttggtgtcaa cggattacgg ttccagtgca caatggccgc cggcgccgtt
    16861 tcgcgccgct ggcaccccgc gagaatcgtg cacgctagca aagtgacaac gattccgttt
    16921 cttcttgccc taaacgtcca gcacttgaat tgcgtcatga tagtttgatg ggtgttgtgt
    16981 accttcgccg cttgctgatc tgggtttcga tcgctattcc tgccctcgcg ggagagtatg
    17041 ccacactttc gaccggcttt tgcctctatg tcgaacgcca cgaaaccgcc ggcgaccggg
    17101 tgcgactcta taccggtcag ggagttaccg agctgcccgt ctcgcaggtg ttagcgttcg
    17161 aaccggagga cgccatcccc ccgcccccct cgtccgcagc cccgaaggag agcccttcgc
    17221 cgaaggagtt ggtcacacag gccgccctcc ggcacggact gccgcctgag ttcgtccaca
    17281 gcgtagccgc caccgagtcg tcttaccggc agcaggcagt gtctccgaaa ggcgcggtgg
    17341 gcgtcatgca gttgatgccg gctacagcca gggagcttca ggccgatcct tcggatgtcg
    17401 ggcagaacat cgacgccggt gtccgctatc tccgtgacct cctggtcaag tatcaaaacg
    17461 acgactacca ggtgcgcaag gccctggccg gctataacgc cggccccggc gccgtagacc
    17521 gctataacgg tatccctcca tatcccgaga cccagcggtt cgtcgagaag gtcatccgcc
    17581 gtcacaagag gctcgcagcc gagccgacgg tccagtaacg ggttcgtcca gaatcactta
    17641 ggctttgcta tactgtgcat agctgccctc ttggaattac gcgtgggctg atccggtcgt
    17701 atgacggccg ggggggaggc aggcgttctt cccgaggctt caagacctat gaacagccgt
    17761 ttcaaatttg cgttcgtgag cttttcggcg tgtctggtgg ttctccttct cgtgggcgcg
    17821 ctcgtcggca agagcgctcc gagcgacgta cccgcttacc gccagatgtc ggtctacatg
    17881 gaggtactct ccaagatcaa gagcgactac gtcgaggaac ccgacctcaa gagcgtcacc
    17941 ctcggcgcga tgaatggcct gcttgaatcg atcgacccat tcgccagcta tctcaacgcc
    18001 gagcaataca aacagtatct gaagaactca gacgcgaaga agggtgacgt cggattggtc
    18061 ctctccaagc gttacggcta cgtcggtgtc gtcagcgcgc tacccgactc gcccggggcc
    18121 aaggcggggc tcacgaccgg ggacctgatt gagagcatca atggcgtcgc gacacgggac
    18181 atgcctctgg cctatgccga gttgcttctg cacggtgaac ctgccactga tgtcgagatt
    18241 tcgattctcc gaatgcggag tccggagccg cagaagatca cgttgactcg tgctctggtc
    18301 tcggtaccgc cggtgacttc gcgcatgctg gccgatcagg tcggctacgt ccgtccggag
    18361 acgctggtga ccggcaagag tgccgaggtg gcggccgcga tacgcactct ccagcagcaa
    18421 ggcgcgaagg gattggttct tgatcttcgt cagtgcgccg tgggcactgc cgaggaaggc
    18481 ctcgccctgg ccaacttatt cctcgagaag ggcctcatgg cctacctgga aggtcagcga
    18541 gtcccccggc agaacttcga cgccaagagc gagaaggcgc tcacgcgtct gccgctggtc
    18601 gtgaccacga accgcggaac ggccgatgga gcggaacttg ccgcaagtgc tctcatcgag
    18661 aacaagcgtg ccgaagtggt cggcgaacgc agcttcggcg atgcctccgt ccgccgggct
    18721 atcagcatgg aggatgggtc ggctatcatc ctttccgtag cgaaatatta cactccgcag
    18781 ggcaaagcaa ttcaggacac gggcgtcgtg ccatcggttc ctttcgtcga gtctgagccg
    18841 tttaccgaga tcgaggatga ggaaaccgcg gaacgtccgg agcccgctcc ggagcagcag
    18901 aaaccagaag aggacatgct tctgaagaag gcgattgaag tcctcactca gggcaaacct
    18961 cagcaagctc tcgtgcgcgg agccgcttcc gcccggcagt agttggaagg gtctgtttgc
    19021 actatgcctt tgtatgaata ccgatgtacc gaatgcggcg acgtgtttga agtgattcag
    19081 aaactctccg atgcgccact gacggtgcac aagaagtgtg gcggcacggt cgagcgtctt
    19141 gtgtctccct cagcccttca gttcaaggga tcgggctggt acgtcaccga ctatgcccgc
    19201 ggatcttcga gcgggagccc ttcgggtaag gacgggaaag acgcgtccaa gagcaccccg
    19261 aagtcggaac ccgccccggc taaggcttcg acaagcgaga agtagccgtc actatcgcaa
    19321 ccagagcgat cccaagtcct gcgagtgcgt ctacaaggta gtggtaccgc ccatacactg
    19381 ttgccgtcgc gattgagacg gcgagaacca gtagaaaccg tcctgcccac ggtctctccg
    19441 gcaagacttg cctcattgcc agcgccgctg aaaaagcccc tgacacatgt gcgctgggga
    19501 atacgctggt gtgaattccg taacctccca aaagccccag gttgaaccgt cgaaacacgg
    19561 tattccacgt gggtagattg tcacccggaa aaacggtgcg gggcggctcg gatggaaagt
    19621 acgggaacag tgcatacgca cacaggaccg ccagcgcgaa tgggaacaga aaccggtcaa
    19681 tccgatcgcg ccggccggtt gccatcagca gcgccagtga gaagtacgga attgcgtaaa
    19741 ccagcgagta ggatatttcc agaacagacg gcagcaccgg gcccagcgac tcgatcacag
    19801 cccgggcgcc cagatcgttc agaaacagcc ggtcccagat tacccacgct cgctcgagcc
    19861 ggaaactgtg cgtcgccggc gcaaaccagc ccatctcacg gtaggccagc agcagcagcg
    19921 gcggaggaaa ccagtctcgt accgttccga ggaaacggcg gcgccgaagc gaatctaccc
    19981 aggccagtag gaacaggccg gcgacgaccg ccacattcac cagcagcgtc acctttgcca
    20041 ctccctccgg caccggcagc agatgcgcca gaaccgtcgc atacgcgaaa tacgttacca
    20101 gcacccactc agaacgtctc agcaagctca ctgtggcatt ctacgccgaa cggttgcgcc
    20161 ggtacgctag agtaaaagta gaaaggaatc actccattct tatgcgcgcg acacgaagac
    20221 agttccttca gggagcggcc gccgccggcg tggcggtcga gcgggcggcc gccggacagg
    20281 cagaccaggt ctctccgaac gacaagattc gcttcgccac aatcggtatc ggcgggatgg
    20341 gctccggaga cacgcgcacc gcgctctcca ttcccggcaa cgagttggtg gccgtttgcg
    20401 atgtctatga tggtcgtctc acccgcgccc gggaggtctt cggcaacaat ttgttcacca
    20461 cgcgcgacca ccgcgaaatc ctctcccgca aagacgtcga cgcggtcatc atcgccacgc
    20521 ccgaccactg gcacgcgcag attgctatcg atgcgctgaa cgcaggcaag gacgtctacc
    20581 tccagaagcc gatggtccag aaagtcgagg acggaaggcc gctcatcgag gcggagaaaa
    20641 agagcgggcg cattctccag gtcggaagcc aatacgtcag ctcgatcatc tatgccaaaa
    20701 cgagagagct gatccaggcg ggtgtcatcg gcgaactgaa catggtcgag gcatggctcg
    20761 accgcaacac cgcgaccggc gcctggcagt attcgattcc gcccgatgcg tcgccggaga
    20821 atattgactg ggacaggttc ctaggcaacg ctccgaagca ccccttcgaa ccgatccgtc
    20881 tcttccgctg gcgaaactac cgcgactacg gaactgctgt cgccggcgac ctgttcgtgc
    20941 acctgcttag tggcctgcac gtcgccaccg gctcgctggg acccaaccgc gtcttcgcta
    21001 ccggtggcct gcgttactgg atggacggcc gcgatgtgcc agacgtaatg ctcgccctct
    21061 tggattaccc cgcgggcgag cggcatccgg cgttcaactt ggcgctccgg gtcaacttca
    21121 agtgcggcct gccggacgag cgtttcgggt tccggtttgt cggaagcgaa ggaacgatca
    21181 ccgcgggttc ttccgtggtt gtttccaaga cccagccgga gagcgaaccc ggttacacca
    21241 tcgggacctt cccgaaagcc gtccaggaag agttcctgaa agagtaccgg aagaagtatc
    21301 cacagaagcc tctgaccgcg gaaagcctca gccccgacca ggaactgacc tacgctccac
    21361 cgcccggcta caacgcgcac ttggaacacc acaaagtgtt ctggtcctcc gtccgaagcc
    21421 gcaaacctgt agttgaggac acgctcttcg gcttccgcgc cgccggcccg gcgctgctat
    21481 cgaatgtcag ctacttcgaa gagcgggtct gcaagtggga cccgcagact ttggaggcgt
    21541 cttgacgccg ggagtcccgc ccaagtggca ctctcatcga aagtcatcgt tgcacgacgt
    21601 ctggacggcc agcgtggttt gtttgtggcg catagcgtgg agcccgatga aatcctgatc
    21661 aagtacgacg gtccgattat cgaccatccg acccgcctgt ccatccagat cgacgacgac
    21721 aagcacatcg aaggcaccga ggattccaac gctttcctga accacagttg tgaggccagc
    21781 gcgtatgtgg attgggacgc cgtctgcctc cgcgccgtga aggccattcc cgccggcgcc
    21841 gagatcacct gcaactacct gacgacggac tatgaactcc acttcccgtt ccattgccgc
    21901 tgtggctcac ctcgctgcaa gggaacgatc cgaggtttca agcacctgac tccggctgag
    21961 cagctcgagc tggagccctg gctcccgccg ttccttaaac gaaaactcca gcgctgccgt
    22021 ccatagtagt tattaagata ctaatatctt ttcatctgta acgttctttt cccttgacat
    22081 ttcgacttca cttgacatac tgttttgcat ctatgcatcc ggaacgacgc agcattgtcg
    22141 gttggttgct tggtggcggg ttcaccgcct ccattctttc cttcctgtac ccggccattc
    22201 gtttcatgaa cccgccctcg atcccggagg cggcggtgaa tgaggcatcc ggcggaaagg
    22261 tacaggacct caagccgaac agcggcaaga tcgtaaaatt cggaagccgc ccggtccttc
    22321 tgattcgggt cagcgataca gagtggaagg ccttttccgc gatctgcacg cacctgaatt
    22381 gcacggttca gtttcagcag tcgagccacc agatttggtg cgcatgccac aacgggttct
    22441 ataacctgaa tgggcaggtt gtttctggac cgcctcccag acccctggat gagtacactg
    22501 tccacatccg cggcgaagaa gtagtgatct cgcgaacctg acaggaggac tcataccggt
    22561 gaccttcagc gacaaaacaa agcgcgcctc aaggtggttc gaggaacgcc tggggctggg
    22621 tggcgtcagg gaattcatcg cgcacaaggc cgtgccggtg cattcggcga ccatgtggta
    22681 ctacttcggc ggtatcacgt tgttcctttt cgtcatccag gtccttaccg gcattttgct
    22741 tcttctctac taccgaccca cgacttcgga agcctacgaa agcgtgcagt ttatcgtgac
    22801 gcgggtccag ttcggctggc tggtgcggtc gattcacagt tggtcggcca acctgatgat
    22861 tctggcggcg ttcatacaca tgttcagcgt cgttttcctg cggtcgtacc ggaggccgcg
    22921 cgaggtaacg tggctgagcg gcatcacgct gctgggactc tcgctcgcgt tcggattcag
    22981 cggctatctg ctcccctgga acaccatctc ctacttcgcc acgaaggtgg ggaccgacat
    23041 ggcggcgagc gtcccggtca tcggcccgcc tctggcccgg tttctccgcg gcggcgacga
    23101 tgtgggcgga gctactctca cccggttctt cgggttccac gtcgctgttc ttcccggagt
    23161 gacgacgtta gtgctcatgc tccaccttct gctcgtccag aagttcggca tcagttcgcc
    23221 gccgaagacc gaagaggaat accggcgccg cggcgaccgg atccctcaga tgcagttctt
    23281 tccgaacttt ttcctgcgtg aattgatggc ctggtatgcg gctctggggg tgctcggctc
    23341 gctggccgcc atcttcccgt gggaactcgg ggcgaaggcg gatcctttcg cggccgctcc
    23401 agcgggaatc cggccggagt ggtattttct cgcgccgttt tacacgctca agcttctgcc
    23461 aagccacatc tggattatcg aaggtgagct tcttggatta atcggatttg ggctgctggc
    23521 cgtcttctgg gtggcgctcc cgttctgggc cacaaatcgc gaagggatgg agcggacgaa
    23581 acttgtgacc ggcgccgggg tggcgttact agcctacctt gcgacctttt ctgccttggg
    23641 gtactggcga tgaaactgag tctgcgaaaa tgctggtgcc ttttcctctt tgctggtctg
    23701 gccggcgccg ctaaggattc gtgcgtggag tgtcactccg gcctcgacgg aaacttacag
    23761 aaaccggcga cccttttcag tgacagcatt caccggcagc gaggctttta ttgcaccgac
    23821 tgccacggcg gtgaccgcac tgcggacgac ccggaggcgt cgatgagccg cgcgcgcggg
    23881 ttcatcggaa agatcccgcg taccgccgta ccgaagctct gcgcgcgctg ccacagcgac
    23941 gcggcactga tgcacaagta caacccgaag cagcgaatcg atcagtatgc tcaatacctg
    24001 accagcgttc acggcaagcg gatagccgca ggcgacgcgg ctgccgccaa ttgcgtcgat
    24061 tgccacagcg tgcacgacat ccgtggggtc agaagccccc tctcgccagt tcacccattg
    24121 cggctcccgc aaacctgcgc gcattgtcac gccgacaaga cgcacatggc gaagtaccag
    24181 attccgacca gtcagttcga cgagtacagg aagagtgtgc attggaacgc tctcgaacag
    24241 cgcggagacc tttcggcgcc gagttgtgct tcctgccacg gcaatcacgg agccacgccg
    24301 cccggtgtga actccgtggc ggcagtctgc ggaacgtgcc acgtgctgat ggaagacctt
    24361 tacagcaaga gcccccacca gccggttttc cagtcgatgg gcgtggcggg gtgcgtcgtc
    24421 tgccacagca accacgccgt gctccccgcg tcggacaagt tcctcgccgg cgaaaaatcc
    24481 gtctgcactc agtgtcatga gcctgcctcc gcgggtggcg ccgcggcagc cgatatggcg
    24541 aaatcgatcg agcgtctcgc cggcgctctg cgccgctccg acgaagcgct gcaacgggcc
    24601 gccagtctgg gactggaggt gtccgaggcg caattgcagc agcaggaagg acgggaggcg
    24661 ctggtgaagg cgcgcgtcgc cgtccacgcc ttcgatccgg cggcggtcaa cgagcctgtc
    24721 agccagggcc tcaaggtggc ggaggcaacg taccgctccg gcgtggacgc attggccgag
    24781 gggaaccggc gccgtgtcgg gcttgggatt tctctgatca cgatcgtgat caccatggcc
    24841 ggcctctggc ttgccatccg gcgcatcgag aaaccgtcgc gtcagggagg actgtgacgt
    24901 atgcggctgg cttccgttgt ctgtgtcttt cttaccgcgg gcgccacttc ggctgccgat
    24961 ctcaaaccgc tcagcagcgc ggaattctgc ggccgctgcc accgggccat cgtggacgca
    25021 tggaaaacct ccgtccatgc caaggcgatg gaaagcccat tgtttcagga tgcgctcgag
    25081 ctgacggaga cccggttcgg atcttcggcg cgaaccgtat gcctggagtg ccactcgccg
    25141 atcgcggttc tgaccaatga cacgtccctg cgacagaaag tgagttggga aggagtgact
    25201 tgcgactact gtcattctct tcgcgacgta tcgttctcag ggaagaaccc caaggcgaag
    25261 gtggagttct cgctggtgaa gagtgggcct atcaaggacg cggaagcgcc ggtccatgga
    25321 acagtcttct cgccggtcca tttatcctct gcggcgtgcg ctccgtgcca cgagtatcgg
    25381 aatccgcagg gcttcccggt actgaccacg tactcggagt ggaaaaatag ccgctacgcg
    25441 aagtcggaaa agcagtgtca atcgtgccac atgtatcggg tcaaggggga tgtcgtcgat
    25501 ccgaaggtcc acaagtcggc caccgccaag gtcaacctgc atcagatgcc gggcagccac
    25561 tcgctggagc agttgaacag taccatcaaa gcccggcttt ccacgtctcg ggaaggagac
    25621 agtctcaaac tgacggtggt tgtgtccaat gtcgcggcgg gtcattacgt gccgacgggc
    25681 tcaccgatgc gccaactttt gctcgacgtg catgccgact cctatgacgg caagcacttt
    25741 caggaagaac gccgctacgc gaggaccgtg gcggaccagc agggtaaaac agtcgatttg
    25801 gagccgttgg ctttcatgag cggtgcccgc gtcgtctccg acacccggct ggccccggac
    25861 gaaacgcgca ccgagacgtt ttcgttccca gtcccttccg gagtcaaggc gcaggtgcgc
    25921 gtcgagctac ggtattacta ctcgccactg gcgacaacgg aggcgcaaaa gcagcttacc
    25981 ttcctcacgc tgcgtcgtct cgttccgtga ttcgtgccgg tgggtgagtt acgaccctgc
    26041 ctgcttcttg agtccgagtg tctcgttgag agagccctgg cggtagcctt ccaggtccag
    26101 agtcacatag tggaagccca gcggcttgaa tatctcgacg aaacagccgg ccatctcggg
    26161 cgacagggcc ttctccattt cgtctttggc gatttccaga cgcaccaact caccgtggaa
    26221 gcgcacgcgg aattgccgga agcccagcgc gcggatctgg tcctcgccct tctccaccgt
    26281 cttgacgttc tcgatagtca cgggcgtgcc gtacgggatg cgtgagctca ggcaggccga
    26341 agcgggacgg ttccacgtgg gcaggcccgc caggcgggac aactcccgga tctccgcttt
    26401 cgagagacct gcttccacca ggggcgcttt cacctcatgt ttcttcgcgg cgttttgtcc
    26461 cgggcggtag tcgccgaggt cgtccacatt gacaccgtag atgatgtgct caacgccacg
    26521 ctgcttccca acctcttcga ggcggctgaa caactcgtct ttgcagtgga agcagcggtc
    26581 cgggttgttg gccacgtagt ccggattgtc aaactcgcgc gtgtcaatgt actcgtgacg
    26641 gaatccgcac tggcgggcga gttcctcggc gtcgcgcttg tgcgattcgg gaatcgacgc
    26701 agagtccgcc gtgatcgcgg cagcgttctc cccgagcgcc accccggcag cccaggccag
    26761 atacgccgag tccgtgccgc ccgagtacgc gacaatcacc ttgccgagac tcttgagaat
    26821 cccgaacagc ttgcgctcct tctcttgaag cgccgctgga tcgagcatag gagtagttcc
    26881 cagccccatt agggtcgcca taacttgaat tatatcggac cggacgaaat tactgtaacg
    26941 gcaacgtcgg ctcgttcccg ttcttgccgt tggcctcgtc ctcggtgacc acgcatgcgg
    27001 ccgccacccg gtcacttgtc tccattttca ccaggcggac gccctgcgcc gatcggcctg
    27061 attgccggat ggccgccgct tcgatgcgaa tcagtttgcc gtcctgagtg atgatcatga
    27121 cgtcggtttc gtccgcgacc gccagcactc caactacctt gccggtgcgc ggcgtgacct
    27181 tcatgttgat cacacccttg ccgccgcggc gcgtcagccg gtagtgtttg agcggagtcc
    27241 gcttcccata gccgttctcc gcaatggaca gcatcagccc gttttcctcg actacctcgg
    27301 ccgccaccag gaaatctccc tccgccagat ccatcgcacg cacaccgcga gcctggcgtc
    27361 ccatcggccg gacctctccc tcggggaagc ggatcgcttt gccctggtgc gaagcgagga
    27421 acatgtagtt cttcccgttc gacaggcggg ccgcgatcag ttcgtcaccc tcatccagcg
    27481 acacggcgat gatcccgcgc gacatcggat tgtcgaattc ggtcagctcg ctcttcttga
    27541 tgacaccgtc ccgggtcacc atgacgatgt actggtcggc gacgaactcc ttaaccggca
    27601 gaaacgcctt cacaacctcg tcgggctgga gattgacaag gttgttgatc tgcttaccct
    27661 tgcccgcggt accggcatcc ggaatgtccc aaaccttcag ccagtacacg cgcccgcggc
    27721 tcgtgaacac gagcaggtag ctgtgcgtat tggcgatggc caggtactcc accacatcct
    27781 cggcacgggt acccatgccg atgcggccct tccctccgcg cgactggcgg cggtaggtgt
    27841 ccaccggcgt gcgcttcagg taaccaccgc gcgtgacggt aacggcgacg tcttccatcg
    27901 ccacgagatc ttcgagccgg atctcgcccg ggtcctcgac gatcctcgtt cgccggtcgt
    27961 ctccgaattc cttctggacc tcgcgcagtt ccttcaccag tacgccgcgc aagaccttgt
    28021 ccgatccgag aatctcttcg tattcggcga tccgcttctg gatctcgacc agttcgtcga
    28081 gaatcttctg ctgctccatg ccggtgagac gctggagctg aagctcgatg atcgcctgcg
    28141 cctgcttttc ggtgaactcg aagcgcccta ccaacccctc gcgagcctct tttggtgtcc
    28201 tcgacgcgcg gatcagattg atgaccgcat cgaggttgtc gagcgccttc ttgaaaccga
    28261 gcaggatatg ctcgcgctcg cgagctttcc ggagcaggta ttccgtccga cgccgcacga
    28321 cgtcggttcg gtggtcgacg aaccgcctga tcgtgtcgac cagtccgagt tccctgggct
    28381 ggccgttgac gatcgacagc atgataatgc cgaagccaat ctggagctgc gtctgtttat
    28441 aaaggttgtt gaggacaacc tcggactgct cgccgcgctt caattcgaaa acgatccgca
    28501 tgccctcgcg atcgctctcg tcgcgaatct ccgagatgcc ttccagcttt ttatcgttga
    28561 cgaggttcgc ggcatgctca atcaggcgcg ccttgtttac ctgatacggg atctcggtca
    28621 ctactatgca ctctcggtct cgccctgtgc gctcgatacc ggccttcgcc cggagtttga
    28681 gctggccgcg gcccttggtg taggcgtcca gtatcccctg tctgccgaga atgaaaccgc
    28741 cggtcggaaa gtccggtccg ggcaccatct cgatgatctt ggcgagcggg atgtgcgggt
    28801 tctggatcag cgccaccgtc gcgtcgatca cttccgagag gttgtgcggc gggatgttcg
    28861 tcgccatgcc gaccgcaatg ccggacgagc cgttcacgag cagattggga atccgcgtcg
    28921 gcaggacttc gggttcctcc tcactctcgt cgtagttggg ccggaaatca acggtttctt
    28981 tgtcgatgtc ttctagcagt gtcgcggcaa tccgcgagag gcgcgcctct gtgtaccgca
    29041 tcgccgcggg cggatcgccg tccacggaac cgaagttgcc ctgcccgtcg atgagcgggt
    29101 agcgcatcga gaagggctgg gccatgcgca ccagtgcgtc gtagagcgcg gtgtcgccgt
    29161 gcgggtggta cttgccgagc acttcaccga cgatcttggc gcacttgcgg gtcgggcgat
    29221 tcgccgtcag gcccatctcg tgcatgccga aaagaatccg gcgatgcacc ggcttcaacc
    29281 cgtcccgaat atcggggagc gcccgtccga tgatcacgct catcgcatag tcgagatacg
    29341 accggcgcat ctcgtcttcg atgttgatcg gcaccaggtt ctgacttccg tcaccgccca
    29401 agggcatttg ggtcggaggc ggactcggtg gcgggggcgg cgttggaggg gcgcccggct
    29461 gctcacgatc ggccaaagat tcctcctcgg aacagactca ttttcttcgt cttttcttcc
    29521 cctcgaaagg gaagcctgtc aaagttctat tttatcagaa aatacaacaa gacaggggac
    29581 ttaacacgat tttggagaag cgctgtcgaa ttagtccatg gggtaaagac tgcacccgcc
    29641 gtccaccagc agaaccgagc ccgtcatata ggccgctgcg gcgctcgaaa ggaacaaaaa
    29701 tgcgtcggcg accgactccg gcgactgaag ttcgccgagc gggatcgcct tttgcgcccg
    29761 cgcctggtat tccgcgtccg cgtcccactg gcgcctggcc attcccgcgc cgacgatgcc
    29821 tggggcgacg gcgttggcgc ggatctttcg gggtgcgagc tcccgcgcga acgaccgcat
    29881 gagctgcttc atcgccgcct tgctcgcgtt gtaggcgcca atatccggcc aaggcacgtc
    29941 ggacacccac gacgtggtga agatcaagtg tcccgcaatg ccgtcttcga tcatcgtccg
    30001 cgccgccgcc cgtgcgagca ggaaggcgct cttcacgttc acggacatcg tccggtccca
    30061 gtctttctcg ctgaaatcga ccaaagaacc cggcacgacc atgccggcat gacacagagc
    30121 ggtgtggata ggaccgaatg cctcgcgcgc atacaccacg aagcgctccg ccgtatccgc
    30181 ttcggcgatg tcgccgcgga agtaccgtac actgcccgcg ggaattgccg ccgcgctcag
    30241 acggctttcc gcttcggact cagccagcag gtcgttcacc gacatccgcg cgccgtgttc
    30301 caccagtgcg cggatgaccg gcacaccaag cgcgccgcaa ccgcccgaga tgacgatgtg
    30361 ctgccccgat agagcgtccg cccgaaaaca cgacatcccc agaccccctg cgattcatga
    30421 cgctacagat ctttcaaccg ctcagtcgcc gtccgaattt gtgaatccag cgttcgcagt
    30481 gcgtccgcca ctcccgccgc gtaccggtcc gcgtcgtcga aacggcccgc ctccacggct
    30541 tcgcgcacgg ctggcagtgg tttggcactg tagccggtgt aaagccccgg agcgtacaga
    30601 cggttcttgt accactcgcg gccgggcagc ccgtctttcg cgagcagtgc gcgctcggag
    30661 cgaaagagcg cctggttgac ttgcttcagt ttctccggtg gcgcgccctg cgcccgcttc
    30721 atcgccttcg cgagttgctt ctcataacgc tgtgcgctct tctcgacgcg cttcagctcc
    30781 cccagaacgg catcgaatga aactcgcccc gacttttttc cggcgacctt atcgatctcc
    30841 agcacatacg accgcacggc gcgggcgaga gtgtcgaact cgaacggcag cacaggcgca
    30901 tcggctagcc gtgcaactgc agttgccatc acctgcgcca ttgcctttcc atacacaaac
    30961 tgcccgtccg aaaagcgcga gtaccaggcg aaggaatcgt agatggagtg atagatgccg
    31021 ccgccgctat ctccactgat ggtgaggttt acgctggcga cgccggcatg atggacgaag
    31081 gcgacgtagt ccgaaccggc tccgagagaa cccagccgga actccggttt ctcctcgggc
    31141 ttgtccttcg agggcttcgg cgtggcggcc tcgagcaggc tcgtcttcgc caccggatcg
    31201 gggacgtcgc gggccacctc gcggatgaac gtatcgagcg tcggtgaggc gccgcctgtc
    31261 agccgcccct tgcccgtact atccgtgttc agataggcga ccgccttcgc ctcgatctca
    31321 tcacgatgtt tctcgaccca ttcggtcgag ccgatcaggc cgaactcctc cgcgtcccag
    31381 agcgccagta ccagcgtccg ttttggcttc cagcctccct tgacaagctc tgcgagagcc
    31441 cgggccgttt cgagcaacgc ggccgcgccg ctcgcagggt cgtcagcgcc gttcacccac
    31501 gcgtcatggt ggttgccgta gatcacccat tcgtccggaa actcactgcc ggcgatggtc
    31561 gccagcacgt cgtataccgg ccgggtctcc atgtcagtcc gcacctcgag ccgcgccgca
    31621 gccgggccgg gtccaaggtg gtacgtcaga ggcagcgctc cccgccagtt ctccggcgcc
    31681 acggggccgc caaggttccg cagcagcggc tcggcgtcgg cgtacgaaat cgggagcacc
    31741 gggattttca tgagggtctt ggcttcgtca ggcgatagcc gacgcgaacc gggctccgag
    31801 gcccaacccg gtgaaagcgg atcgccgaca tacaacggca tatccatgac gctgccgcgc
    31861 tgcgcccctt gcggaggccg aaaagcgcct ttcggatata cgtcaccctg atagaagccg
    31921 tcgtccctgg catccgagta gatcaggcaa cccaccgccc cgtgctcggc cgccaccttg
    31981 ggcttcgtgc cacgccagct tccgccgtag cgggccatca caatcttccc gcgaacatcg
    32041 atcccgagct gtttgaggta ttcgtaatcc tccgggattc cgtagttgac atagacaaga
    32101 ggcgcggtga cgactcccga tccggagtaa gcgttgtagg tcggcaattg acctgcatcg
    32161 ccggagtccg gatcctcgga gatcgccggt tcggccagct tcgcggtgta ccgctccggc
    32221 tccaccattt cgagcgaccg cttcacgggg tatggcagga gcgcctcgaa gacctcaatc
    32281 cgggcgtcga gcccccagct cttcatcaac ccgagtgcgt attccgcgac ggccttcgag
    32341 ccaggggagc cggcgtgatg cggctcggca gacatccgcc ggacgtactc gcgaagacgt
    32401 tcggaagacg gtactgcgcg aagccttgac tcgagatccc ggcgagcggg ccactcctcg
    32461 ggagggaacc cgcgaagcgg accttcggcc atcatgatca ccgacgataa taccagcaga
    32521 atcaatggct tcattcttgt ttctgaactt ttaaattaag atctggcgcc attctgccca
    32581 gcccggagga aacgcagaaa ctcctcggtg cttcgggtca gtcccgcgaa gctagcgatg
    32641 accttcccgt cggcgccgac gatcgcatag aacgggatgg cggaagtgga gaacttgtcg
    32701 gatagaacct tctggttagc ctccgaggct tcgtcggttc catctgtgta aagctccacc
    32761 aggacaaagt tgctcagcgc ttcggcgatc tgggtacgcg ggaacatgtt cgccttcatc
    32821 cagtggcagt tcgtgcaggc gtagcccgtg aaactcaaaa gcaccggctt cccttcctca
    32881 cgcgccttcg ccagcgcctg ctgatactgg ttcttcatcc agaccggtcc ggatacgccg
    32941 gccgtcccgg aagaggggcc caaagtcgtt ccgccaatcg gcggcggcac gtacgcgtca
    33001 agttcgccga gtctaccccc gaacatcccc ggaatcaggc tcacggcaaa cacaaggaag
    33061 atcgtgccga tgcacaaccg ccagatgcca accttgtcgt cccgagcaat tccctccatc
    33121 cgaagaaaac cgagcaaata gagtccaggg agcacaaaca gaaccaccca ggcggcaaga
    33181 aacctctctc tggtgaggaa gttccactgc atcaccacgt caatgttgct gacgtacttc
    33241 agcatcgctg ccaaaatgac gaacccgaga accactttga cgcgggacat ccagccgccg
    33301 cttttcggca gccgtttcag gtaggccggg aacagcgcca aaaggaagaa cggtgcggcc
    33361 agacccgcag cgaacgaggt catcccgacc agaggacggg ttccaccgcc cgacaccgag
    33421 cccgccagca gcgtccccac gaacggaccg acgcaggcga acgccgttag cgagaatgtc
    33481 agccccatga tgagcgttcc cacaacgccg ccaccctgcg aggcccgatc gagccgcgtc
    33541 agcaggccag atggcagagt gatctcaaag gcgccgagaa ggctgaggcc gaaaaccaaa
    33601 aagacaagtg aaatgaatcc gttgacccat gggttcgaac ctaattgctg cgttccaaac
    33661 ggacccaata tggccgtggt caggagcccc agtccggaga ataggacgat gatgccaaga
    33721 cagaaaacgg ttgcctggaa gacgctctgt cccttcgaac ccgactgccg gttcagaaag
    33781 aacgacaccg taatcgggat catcgggaag acgcaagggg tgaagaccgc cgccaggcca
    33841 aaaccgaagg cgacgagggc aaagcccagc agcccttggt cctgtacccc acccatcgtc
    33901 gagattggct tcgtttcgtc ggagacgggc gccgttgcgg cgggcttccc cggctcaaac
    33961 agggtatagc ctgccggcat ggtcgctttc ggcgcggggg cgaccgggtc aatcgtcaac
    34021 gtcgcggaag ccgtctttcg gaccggaatg agacactgtt ttgcatcaca ggcctgataa
    34081 cgcacctggg ccgttagttc gaccgaacct gccggaactt ctttgccgac ctgtacttcg
    34141 atgagaaaaa tagcttcatt atcatagagt tcagtgtcta gcttaaagtt tgggtcgaac
    34201 ttgacagtgg gcttcggctg gtgaatccgg taagcggtta cggcggggtt gtcggcgatg
    34261 cgaatggtgg tcgggattgg tcccttcggg gtcgacagcg agtagaggtg ccagccggag
    34321 tcaattttgg ccgcgagccg tgcgaccacc gatgacccgg gcggcgcctt tgccgcctcg
    34381 accgacatcg accatttgac cgggtcgagc cgctgggccg gcatcaccca cggcagcgca
    34441 aacccggcaa gaatcgcaaa cgcggttttc gggaccatcg ttctttagac ggctttcacg
    34501 gccgcggcga ctgtagggga cttgagcagc tcgctttcga tgcggctgtt gaggttgccg
    34561 ccggcgaatt ccgccgccac ccggatcgcg ttctttatgg cgtttgtgtt agagcggccg
    34621 tggcagatga tgcaaacgcc cttgacgccc agtaggggcg ccccgccgta ctcggagtag
    34681 tcgaggcgct tcttgaaatc cttgaacgcc gcccgggaga gcacatatcc gatcttgccg
    34741 gtaatagtcg cttccaacga ttcctggagc atttgcttga tgatgtccac cagcccctcg
    34801 ctcaccttga gagcgacatt cccgatgaac ccgtcgcaca cgatcacgtc ggcggacccg
    34861 ctgtatacgt cccgcccttc gacattcccg atgaagttca gcggcagggt tttcagcaat
    34921 tggaacgcgc cgcgagtcag ctcattgccc ttgtgttcct cctcgccgat ggagagcagc
    34981 ccgacacgcg ggttgggtat ccggatgatt aagcgggcgt agatatcgcc catgatcgag
    35041 aactgcgcga gcattctcgg cgtgcagtcc acgttggcgc ccacgtccac catcacggcc
    35101 ggggttccgt tcatggttgg gaagacggtg gccagcgccg gccggtccac tccgggcacc
    35161 atgccctgca ccatcttcgc ggtgatcatc accgcgccgg tgttcccggc tgaaacgact
    35221 ccctgcgcga ctccttcctt gacgagccgg cacgccacgc gaatcgagct gtcccgtttg
    35281 ccgcggacgg ccttggtcgc actatcctcc atcgtgacct gctcgctcgc gtggacgatc
    35341 tcgaccggaa gggaccggta gccatcgtgt acagcaagct cgcgacgcag aagatcctca
    35401 cggccgacca gcatcacccg gacaccgaga gtccgggcag cccgaatcgc accttcaatt
    35461 tcggtcttcg gggcgtggtc gccgcccatg gcatcgaccg cgattgtcac catagacagg
    35521 gagaagttct tcgagcagcc ggcaattctc tactcttcag cgacttcaat cacctcacgg
    35581 cctttgtatg taccacagtg cgggcacgca cggtgaggca gcttcggttc gtggcagttc
    35641 gggcactccg aaagcccggg ctgtttcagg tagtcgtgag cgcggcgggt cgacgtgcgc
    35701 tccttcgagt gtcttcgttt cggattcggc atcggtcaat atccttaatc tgaaatctgg
    35761 tcgcggcgta ttccgccagc ggcggcttac agatttttca acgccgccca gcggtcgtcg
    35821 agcggttcag cccggcagcc gcaatcggct aggttcctgt tctggccgca aaacgggcaa
    35881 attcctttac agtccacgcg gcaggtccgc tgcatcggca gtgccagcag gatctgctcc
    35941 cggaggatgt gctcgagttc gagcccctcc ccctcgtaaa aatcaatctc gctctccccc
    36001 gcgccgagtt cgagttcgtc cttgtcgggc aattcagcgg ccggctcgta gaacagatcg
    36061 aaactgacct cgatcggaaa acttgcggtc tccaggcacc gatcgcaatc ggcttccatt
    36121 cggaccttga ggcgtccccg gatccggatc tccccgttcg tattgggaag cagttccgcc
    36181 gtgccggagg tttccagtac cgacgcctgc cggatcttcc cttcaaggaa ctgaatctcg
    36241 ccgggcggga atgcctcctc aaagcgcagc ttccgcagtt ccatctcatg cacgctgagg
    36301 aacatggcag cctcccgggg gtcctgaatc cgcgcagtta agcctttatt atagccgctt
    36361 ttgcagagtg tgcgcggcct tcgacgcctt acggctccgc gggcggcagg aagcgccgca
    36421 ctccagcctc atagatccac gcgcccagca ggccgcccgc gagcggcccg acgataggaa
    36481 cccagaagac gtagctgccg tccgtgaggc cgttgttccg gaatcccgcc agcgtcacaa
    36541 acagccgggg cccaaggtcg cgggccgggt tgatagcata cccgtgcagc gagccgaaag
    36601 aacagccgat cgcgacgact acaagaccga tcgcccacgg ggcaaggccg cctggaaccg
    36661 gttggttgcg ttcgtcgacg atcgccatta cgaggagaag aagcaaagcg gttcccagaa
    36721 tctggtcgaa caaaccggcc atcggcaatt ggggaaaggc cgggaaggtc gtgaagatcc
    36781 cagccgtgcg ctcgagcgtg gggtccgcct tctcaaaggc gggcctgtaa ttcaggaaga
    36841 cggtggccgc agcaagaaat gcgcccgccg tctgcgcgag actgtacggc agtactttgc
    36901 gccacgggaa tccccagaac gcggcgaagg cgaccgtcac cgcaggattc aggtgtgcgc
    36961 cgcttatcct ccccgccacg tacacgccca tcgtcacccc gagaccccag ccgagagtga
    37021 tgttcgtcca acctccccgg acgacctccc cgggcacacc cgtgctgaac aaggtcgtca
    37081 tggcgcccac gcctgcgccg aaaaggatca gcacgaaggt tccaagaaac tccgccagca
    37141 gctcaccacg caatgaggac acacgcattg tcggcgccct gccccctaac tcacgtagct
    37201 gtcgaacacg cgaacctggg accagttcgc cacattttcc tggatgaact gcttgtgctt
    37261 ctcgtgaacc tgatagcggt catgatccgc tttggccttg aacactacgt tcagggcgac
    37321 atcgaagccg aggtcgttgg cctcccgttt gaagtcgacc gcccgtgaac cggcagcgaa
    37381 gaagacgacg ccctcgtggt ccttcaggta cttgtggcag gctgcgacca gatcggcctt
    37441 cgccgcatcc gacgcgtcct tcaggctgaa gtagacgttg tggaccagca tctctccgtc
    37501 cgcggcggac aggacggcgg agccggaaca gaagagggcg ggagcggcca gtaaggctct
    37561 tcgctttaac atgtagcctc cgtaagcgca gtgactatcc acaacatcat gtcacaagtc
    37621 gcctcgcggg gatgctcgct aagattacct tccttgccgc tgctaactcc tttcgtacac
    37681 ctcggtccaa ttgagctctc cctcgggccc tttccgccgg atggttcgcc gcatctgttt
    37741 gccatcccgg gagaggctag tacgagtggt ttccgaccac tgtccgtcgt cggaccggaa
    37801 gaccgactcg gtggtccagt tgttgatccg ctggatgacg actttccctt tgccaaaaga
    37861 gcgggtgatc ggatactctt tgccatcaat ggccccgctg aaggcgaatg tgcgggtgtc
    37921 ctcgccgccg gaatacacca tcgtccccga gtacttgaga agcggttcct tgtgctcgac
    37981 cgtgaccagg atactgaagg gcttctgcag gttgccccaa cgcgacttct gcacgttgag
    38041 acgccatgtt ccactgaggt cggcgatctg cgccgagcag accccaacag agaaggcagc
    38101 cagcaagacg gccgcccgga aactctgcag cctcatgact cttccctccc ggtttcgcag
    38161 tttaactccg tacgacgccg gcggaaagcc ccggcagtgg ctcgccgggg taagatgatt
    38221 ctcaccgcgc gaaagagtcg catccatgca ggatttgaac ctcgaacacg ttccacctcc
    38281 gccgccgagg aaagattacc gcatcggcgt gatcggcgcc gggttcattg tgcgcgatgt
    38341 gcagttggtt gcatacgcca acgcccggta taacgtgcgg gccatcgcat cagcgaaccc
    38401 ggagcaggca cgggcagtcg cggcgcacca cggcattcca aaggtctatg acacctggcg
    38461 ggaactggcg gccgatccat ccatcgaggt cgtcgacctg gccgtgccgc ccggcgagca
    38521 acctgccatt ctgaaggaac tcccgcgtat cggcccacag ctcaaaggcg tgctggccca
    38581 gaaaccactg gctctgaact acgcccagtc gaaggaggcg gtccaggcgt tcgacggctc
    38641 cggcatcacg ctctccgtca accagaacat gcgatacgac cagtcgatcc gcgcgctgaa
    38701 gtcactcctc actcgtggcc ggctggggga accggttttg gccacgatcg agatgcgggc
    38761 cattccgcac tggcagccct ggagccggaa atacgggcgt ctcaccctgc tcatcatgag
    38821 cattcaccac ctggacgcct tccgctacct gttcggcgat ccggagagcg tctatgtgag
    38881 cgtccgcacg gatccgcgaa cgaagttccc gcatcgtgac ggaataccgc tgtatatcct
    38941 cgagtacgcg agcgggctgc gcgcctccgc ctgggacgat gtctgggcag gacccgcccg
    39001 ggagggcgcg gcctcggaca tctacatcaa gtggcgggtc gagggcacgg agggaatggc
    39061 gtggggtacg atcggctggc cgtcctatcc gaacgcaacg ccgagcacga ttcacttcac
    39121 caccacccgg gagccgggcg tgtggttctc gccccagtgg aaggaagtct ggttccccga
    39181 cgcctttgaa ggaaccatgg gacaactgct gaccgcgatc gccaccggcg ccgagcccgc
    39241 cgtgaccgcc cgcgacaacc tccgcaccat ggcgcttgtg gaggcctgtt accgctccat
    39301 cgacgagcac cggccggtga gtcttaagga gataatgtcc gaatgaacct gggactgatc
    39361 aactccgctt ggcttggctc cggaatcggc tccgccgaag gcatccgcaa gaccaaagag
    39421 atcggcttcg acacgatcga catcttcgcc gacccgctcg aaatcgacgc acgcgagcgc
    39481 aagctgatca aagaaacgtg ccgtgaagtg ggcctgccga tcacctccgt ctgcgcggtg
    39541 gcgctcggga ttgccgactt caacacaagc gtccgccgct ttcacgccga gcgtctccgc
    39601 cggtacgcag atctagtgta tgagttcgac gggaagaacc tgcttaccgt cgttggcgaa
    39661 tatgtctggc agcaggaggt gatcaagccc gaggaccagt ggggctgggc ggtggaggag
    39721 gtccgaaaac tgggcgagta cgccggctcg ctcggcatcg agatcgccat cgaactcgag
    39781 ccgttccacc tttccatcgt caattcggtg gaccgcatgg cggcgttcct ggacgacgtc
    39841 ggccatctcg cggtcaaggc gaatctcgac gtatctcacc tttctctcgt gcggaccgcg
    39901 ccggaggaga ttcagaagtt gaaaggccgc gtcgcacaca ttcacttctc agactgcgac
    39961 gggaaggtac acggcgacct ccctccgggc cgcggtgtgg tcgatttccc gccgtacctc
    40021 cgcgagatcg caaacctcgg cttcgacggt acgctcagca ttgagctcga atactcgccc
    40081 gagcccggcc ggatcatgga atgggtgacg gaagcctatc gggaaaccgc ccggttaatg
    40141 aaggaagcgg gactgcaccg cccgtgaagc tgcccctcgc cgcgacggca gccagcgcga
    40201 atttgcgaag atagtcgttt cccggtaact cctatgtccg aatggtcgcc ggcatcatgg
    40261 cgcgaaaagc cggctttaca acaacccgtt tacccctgcg ctggggaatt ggagagcatc
    40321 ctgcgcgagt tgtcgaactt gcccccgctg gttacgcagt gggaagtgga tgcgctcaag
    40381 gcccaactgg ccgacgcggc gctcggccgg cgttttctct tgcagggcgg cgactgcgcc
    40441 gaaagcttca acgattgcaa cgccggcacg atcgcggaca agctcaagat cctgcttcag
    40501 atgagcctgg tgctcgtcca cggcagcaat cgtcccgcga tccgcgtggg acgattcgcc
    40561 ggccagtatg caaagcctcg ttcggaggag ctcgaaacac gagacggcgt gagcctcccg
    40621 agctatcgcg gcgatctcgt gaaccgtccc ggtttcacgg cggcggaccg gacgccggac
    40681 ccccggttcc tgctcagggc acacgggcgc gccgccctca cactgaactt catccggtct
    40741 cttgtaaagg gcgggttcgc cgacctccac caccccgaat actgggatct gggctgggtc
    40801 aggcactccc ccctggcgtc cgagtatcgc cgtatcgtcg agagcatctc cggtgccctg
    40861 cagtttcttg agaatgtgct cgggatccgt gccggtgaga tcgaacgcgt cgatttcttc
    40921 accagtcacg agggtctgca ccttccgtat gaggaggcgc agacgagccg gtctgacgcg
    40981 ggcggccgat ggtacaacct gtccactcac ttcccatgga tgggtgtgcg aacgagcgat
    41041 ccggatggcg cccacgtgga gtacttccgg ggcatcgcca atccgatcgg cctgaaagtc
    41101 ggcccttcga caagccctgc gacgcttgta catctgctcg acatcctgaa ccctgccgac
    41161 gaacctggcc gtctcacgct gattcaccgg ttcggctgcg atcggatcgc cacctgcctt
    41221 cctcccctga tcgaggccgt aaaggcgagg gggaggacgg ttctgtggtg ctcggatccg
    41281 atgcacggca acacgctgca aacggctgac agggtcaaga cccgccggtt tgccgacatt
    41341 ctgagcgagc tcgaacagtc cttcgacatt cactccgcgc tcggaagcca tcttggtgga
    41401 gttcacttcg agctgacggg cgagaatgtt accgagtgca tcgggggcgc acgcgggctc
    41461 accgaagagg acttgggacg tgcctacaaa tccgaggtag acccgaggct gaactatgag
    41521 caagctcttg aaatggccat gttgatcgcg cgaaagatga aaacgcttgg tggacggtag
    41581 gctccgtcac ccttccgcaa ggccgctcaa gtactcacgt tccttcgccc gaatctccgg
    41641 caaccctacg agatcattga actcctcgaa ggcgaccatc cgcccctcca gacctgatga
    41701 ggttccggtg tcccggatgt gccggaatac gtcctgcgca gtcttcgcca tcgcataggt
    41761 caaagccgtg gggtgggcga cgacggcaaa accgatctcc tcgagttgtt ttgcaggtag
    41821 gattggggtc ttgccgcccg gaaccatgtt cgccatgttc ggagctttga tcgcggaggt
    41881 gatttggcgc atctgctcga tcgattgcgg cgcttcaata aaagccatgt ccgcgccggc
    41941 ttcgatatag cgctgcatgc gggcaatcgc ttcgtcgatt ccgtgcatcg ccaacgcatc
    42001 ggtccgcgcc atgaccatca ggtcggcatc gattcgggtg tccaccgcag ccttgatctt
    42061 ggccagcatc tcttcgatcg gaaccacctg ctttcccgac atgtggccgc aacgcttggg
    42121 tgccacctgg tcctcaaaga aaatcgcggc gacgccggcc ttctcgaact ggcgcatgct
    42181 ccggatcagg ttggtgacgt ttccgtgacc cgtgtcgccg tcggcgaaca ctgggatgtc
    42241 cacggcatcg acgatccggg ccgcgcactc caccatctcc gtcatcgtca tgaggccaac
    42301 atcgggagct cccaaagtgg ctgcgctgct tgcgcacccg ctcacgaaga cggccttgaa
    42361 gccgacctta gaggcaatcc tggcgcaaag gggatcgggg acaccgggga tcacaaggat
    42421 ctcgggatcg tggatgtatt tcttgagtaa agtggtcttt ctcatgtctc gtggggtag
//
LOCUS       DATIFQ010000253         8725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_80856, whole genome shotgun sequence.
ACCESSION   DATIFQ010000253 DATIFQ010000000
VERSION     DATIFQ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80856"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..419)
                     /locus_tag="VLE22_11790"
     tRNA            complement(343..419)
                     /locus_tag="VLE22_11790"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(383..385),aa:Met,seq:cat)
     gene            complement(493..1590)
                     /locus_tag="VLE22_11795"
     CDS             complement(493..1590)
                     /locus_tag="VLE22_11795"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSB15133.1"
                     /translation="MTSKILLLVALVAWRSSASAQPPTLAAEQIERIEKAISAEMSRQ
                     SIPGMSAAVATGRQLRWMAGYGLADVENYVPAKAATVYRLASISKPITAVAVMQLVER
                     GKIALDAPIQRYVPDFPKKRWTVTVQELLCHQGGIRHYRNESELESTRHYASLREALS
                     LFVKDPLVYAPATRFLYSTYGYNLLGAAVEGVTSMKFADYVRANVFEPAGMHTARPDD
                     VYEIIPNRARGYRKRLGGKLENAALIDTSNKVPGGGMCATVEDIADFGIHVQFGQLVK
                     QETVAQMFTARKTRDGKATPYGLGWYILDVAGEKLVEHGGAQPGFRTLLLMLPGREFT
                     VVLMANLEGVNLRPLALEIARIVLPSAQNPK"
     gene            complement(1598..2860)
                     /locus_tag="VLE22_11800"
     CDS             complement(1598..2860)
                     /locus_tag="VLE22_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HSB15134.1"
                     /translation="MIQATFALPEAAKAIAESLLIGFLIGAQREASHGERHAGVRDFV
                     LIALVGAICGLLQAPWLTVAALFSIAALLAVFYFHVGERTGITTEMAAVATFCLGYLT
                     ATPDNPLGALLAIGTAIAVVAFLEAKRSLHKLLRETITETEFNDTLRFLTIIFIIYPL
                     LPDGSYGPFGFLQPRTIWLFVILVSSISYAGYFVEKFLGAQRGMKFVAIFGGLASTTA
                     ATTAFARSSVDAAQDRPLYSQAAVIANAIQFPRVLVIMAVVSPRLAHACLAPLATMCV
                     AGLLYALVILRRAPREATGGDAGVSNPFRLLPALKFGAVFAVILFASKAAAKLYGSGA
                     VYWTSAIGGSVDADAVVVSVAGLLSVGGITLDVSATSVLLALLTNGVLKAGIAFYAGT
                     RTFAWRLTGGLAAMFGAGWLVFWAMGKL"
     gene            complement(2862..3641)
                     /locus_tag="VLE22_11805"
     CDS             complement(2862..3641)
                     /locus_tag="VLE22_11805"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HSB15135.1"
                     /translation="MNRRHFLAGAAGAGFVPPSASAASKKAIFELRNYRLRNTHANQV
                     RRTSDFLEKAAVPALRRAGAGPLGFFSGVIAGDSPFILALVSFPSLSAMESAMEKMAA
                     DSAFEKAREEYNAAPDLGYMRIENSLLRGFESMPRIEVPPSEGNRAARIFELRTYESN
                     DGSTLRRKIKMFDDDEIAIFRRCGILPVFFGETIVGTHLPNLTYMVAYDDLAARDKAW
                     SAFGADPGWQRLRARPDLADAEIVSNISNAILRPLPFSDIR"
     gene            3805..4758
                     /locus_tag="VLE22_11810"
     CDS             3805..4758
                     /locus_tag="VLE22_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HSB15136.1"
                     /translation="MALSNDDHLATLSKDLIQALDNLSGLHPGFRPAHAKGIMLSGVF
                     HPAASAGSLTRAPHVQRQSTPVTVRFSDSGGVPTVADNDPNVAGPRGIAIRFHLAEHV
                     HTDIIGHSTDGFPVRTAEEFLEFLRARHASGPGAPKPTPVESFLATHPAALKFVQTPK
                     PIPASFAKESFFTVTAFKFTNKAGNSRYGRFRIRPDGGAEYLDAQAAAAKSANFLFDE
                     IVERLAKHPVTFHIMVQLAVDSDTVDDSTVQWPDDRAQIEFGTIVLTERIPEDDVEKR
                     RIIFDPIPRVDGIDSSGDPLLDVRAAVYLMSGRRRRAAGAS"
     gene            complement(4797..5516)
                     /locus_tag="VLE22_11815"
     CDS             complement(4797..5516)
                     /locus_tag="VLE22_11815"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF011412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HSB15137.1"
                     /translation="MQSTQYPALHVAIIMDGNGRWATERGLPRVAGHREGSKAVRRTV
                     EAAPALGIATLTLYAFSSDNWRRPRAEVEALMRLFEEHLQSETGRCIENGVRIGVIGR
                     RDRLGTRLASAIASAERSTARCTRLHLRVAVDYSARDVILRAAQRVQGRHDTTRETFA
                     RLLAEAEHSPGPVPEVDLLIRTAGEQRLSDFLLWESAYAELLFVPKFWPDFEANDLAA
                     AVKEFRNRERRFGALPVAAAG"
     gene            complement(5553..5939)
                     /locus_tag="VLE22_11820"
     CDS             complement(5553..5939)
                     /locus_tag="VLE22_11820"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSB15138.1"
                     /translation="MVAKQSALRKEQTQRESGDSRTLREFPAEAAPPALDRLIHERMR
                     LGIVSALAVNESLTFSELKKLMKTTDGNLSVHARKLEDARYITCTKSFDGRMPKTEYR
                     LTALGRRAFERYVAHMEALIEATRGR"
     gene            complement(5929..6543)
                     /locus_tag="VLE22_11825"
     CDS             complement(5929..6543)
                     /locus_tag="VLE22_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15139.1"
                     /translation="MGSVRPIRSEPAVPIELHAHAMDNIRFIRETMEGAAAFTAVPGK
                     GGVAMGLTAVAAAVIAARQPDTERWLVTWLAEGFVAFVLGVFAMAHKARAAGTSLLSR
                     PGRKFGLGLAPPMLAAGLFTLVLYQAGRSALMPGTWLLLYGVGVASAGAFSVRVVPAM
                     GLCFMAAGAAALFAPAAWGNWLLAAGFGGLHIVFGILIAWRHGG"
     gene            complement(6602..7336)
                     /locus_tag="VLE22_11830"
     CDS             complement(6602..7336)
                     /locus_tag="VLE22_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15140.1"
                     /translation="MRNAILSAILALTLVPAMAQEKEQVTKIIEVRNLDVRELPPLLL
                     PFGAAIQFSLPLRRLVVSGRPETVAAVEEAVRRMDVPAPAPKNIEITAYILMASAQPG
                     GSVPPELEGVTKQLKNLFSYQGFRLLDTMVLRVRDGESGEASGVGAPPTDFPTNLSAK
                     TLYQAKFTSVGVSGDDKGRMLRIRELRFGARIPYATGGSGYQFTEVGFNTGIDVREGQ
                     KVVVGKANIDGSNSAMTLVVTARVAE"
     gene            complement(7339..7902)
                     /locus_tag="VLE22_11835"
     CDS             complement(7339..7902)
                     /locus_tag="VLE22_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSB15141.1"
                     /translation="MSCPKFERLIALSVEGDLDARNARLVQEHLGECAACRELHEELL
                     GSQAVLKALNEDAIDPAVLGAVRRRVLERIEDTRPSWWLAWRWRHAVAVGLTAVAVAG
                     VALWPERQVPPPPAPAPVAEVREPVAEHASEQRGADPGIEPRPVRKAVKHAPRKPAPP
                     RGEPLVVKMLTDDPDVVIVWLIDKNGD"
     gene            complement(7899..8486)
                     /locus_tag="VLE22_11840"
     CDS             complement(7899..8486)
                     /locus_tag="VLE22_11840"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB15142.1"
                     /translation="MRELALTMTAAAEASESPEPVILLRLAKAGDVAAFEQLIVLHER
                     RVFLIALRLLRRREDAEDAAQEVFLRLHKHLRRFDDTREFPPWLYRVTVNVCRDLARS
                     RRRDWAVEVGEQRQVEDPTAEYERREQKRIVEEALGRLPEKERAAIVLRDLEGLPTKE
                     VARILGSTAATVRSQVSSARLKIKEFADRFRRRRT"
BASE COUNT         1638 a         2959 c         2533 g         1595 t
ORIGIN      
        1 aagcagcatg aattaccgaa cgtgtgtccg aaggactact ggcaattgag tgagggcgcc
       61 cgccgcacgc tgcgccggat gcgccgacgc cggacactga tcaccgcatt ttggcagcag
      121 gcttctttgt ggcccgagtg accttatatt atgcgggact caataggttg ccgactttgg
      181 ggtcagagac agtcacgatg taggggttat taccgcagtg ccattttctg tgccgacgag
      241 gagcacaggc ggatgctgca aggctatgac ggacaccaaa cgtcaggcgc tattgggagc
      301 ttgtgttgcg ctcgaaagtc tgcagcaacc gattgaaagg gttggttgcg gggggtggat
      361 ttgaaccacc gacctttggg ttatgagccc aacgagctac cagactgctc caccccgcat
      421 ctagatggta gcgcagcccg gtgtttgggt caatctcttt tcggatacgc gactcctgcg
      481 acggacggaa cctcacttcg gattctgtgc ggagggcagc acgatccggg cgatttccag
      541 cgccagaggc ctcaggttca ccccctcgag gttcgccatc aacaccaccg tgaactcgcg
      601 gccgggcagc atcagcagca gggtccggaa gcccggttgc gcccccccgt gctccacgag
      661 tttctcgccg gccacgtcca ggatgtacca gcccagccca tatggcgtcg ccttcccgtc
      721 ccgtgttttt cgcgcggtga acatctgcgc caccgtctct tgtttcacca actgcccgaa
      781 ctggacatga atgccgaaat ccgcgatgtc ctccaccgtc gcgcacatcc cgccgcccgg
      841 caccttgtta ctcgtatcga tcagcgccgc gttctctagc ttccccccga gccgcttccg
      901 gtaccccctc gcccggttcg ggatgatttc gtagacatcg tccgggcggg cggtgtgcat
      961 ccccgccggc tcgaacacgt tcgcccgcac ataatcggcg aacttcatgc tcgtcacacc
     1021 ttccaccgcc gccccgagca ggttgtagcc gtacgtcgag taaaggaaac gggtcgcggg
     1081 cgcatacacc agcgggtcct tgacgaagag cgacagggcc tcccgaaggc tcgcgtagtg
     1141 acgcgtactc tccagttcgg actcattccg gtagtgccgg atgccgccct ggtggcagag
     1201 cagctcttgc accgtcaccg tccaccgttt cttcggaaaa tcgggcacgt accgctggat
     1261 cggagcgtcg agagcgatct tgcctcgctc cacaagctgc atcaccgcca cagccgtaat
     1321 cggtttcgag atcgacgcca gccggtacac tgtcgccgcc ttcgccggga catagttttc
     1381 gacgtccgca agaccgtatc ccgccatcca gcgaagctgc cttccggtcg ccaccgccgc
     1441 tgacattccg ggaatgctct gccgggacat ctcggctgaa atcgctttct cgatccgctc
     1501 tatctgctcc gcggcaagcg tcggaggctg cgcgctcgca ctggaacgcc acgccaccag
     1561 cgccaccagc agaagaatct tcgaagtcac cttcatctca aagcttgccc attgcccaaa
     1621 acaccaacca gcccgccccg aacatcgccg ccagtccgcc cgttagccgc cacgcaaacg
     1681 tcctggttcc cgcgtaaaaa gcgatgccgg ccttcagcac tccgttcgtc agcagcgcga
     1741 gcagaactga tgtggccgag acgtccagtg tgatgccccc taccgacagc agccccgcca
     1801 cggaaacgac taccgcatcc gcgtccaccg agcctccgat cgcgctcgtc cagtaaaccg
     1861 ccccgctgcc gtaaagtttc gccgccgcct tgcttgcgaa cagaatcacg gcgaacaccg
     1921 ccccgaactt aagcgccggc agcagtcgga acgggttact cacaccggcg tctcccccgg
     1981 ttgcctcccg tggcgcccgg cgcaggatca ccagcgcgta aagcaggccc gccacacaca
     2041 tcgtcgcgag aggcgccagg cacgcgtgcg ccagcctcgg actaaccacc gccatgatca
     2101 ccagcacgcg cgggaactga attgcgtttg cgatcaccgc cgcctggctg tacaggggcc
     2161 ggtcctgcgc cgcgtccaca ctgctccggg cgaacgcggt cgtcgccgct gtcgtcgagg
     2221 cgagcccgcc gaaaatggcg acgaacttca tcccccgttg cgcgccgaga aacttctcga
     2281 cgaaatagcc ggcataggag atcgacgata ccaggatgac aaacagccag atggtccgcg
     2341 gctgcagaaa accaaacggt ccataggagc catccggcag cagcgggtag atgatgaaaa
     2401 tgatggtcag gaaccgcaga gtatcgttaa attcggtctc ggtgatggtc tcccggagca
     2461 gcttgtgcag tgagcgcttc gcctccagga acgccaccac cgcgatggcg gttccgatcg
     2521 ccagtagcgc gccaagtggg ttgtccgggg tcgcagtcag gtagccgagg cagaaggtcg
     2581 ccaccgccgc catttcggtg gtgattccgg tgcgctcgcc gacatggaag tagaagaccg
     2641 cgagcagcgc cgcgatggag aacaacgcag ccacggtcag ccaaggcgcc tgaagcagtc
     2701 cgcagatcgc acccaccagc gcgatcagga cgaagtcgcg aacacccgcg tggcgctcgc
     2761 cgtgtgaagc ctcgcgctgc gcgccgatca ggaatccgat caaaagcgac tcggcgatcg
     2821 ccttggcggc ttccggaagc gcaaacgttg cctggatcat gctaccggat atcggaaaac
     2881 ggtagcggcc gcaagatggc gttgctgata ttggaaacga tctccgcatc cgccagatcg
     2941 ggccgcgccc gcagtctctg ccagcccgga tcggcgccga acgcgctcca cgccttgtcg
     3001 cgggccgcca agtcgtcgta ggcgaccatg taggtcaggt tcggcaagtg agtgcccacg
     3061 atcgtctcgc cgaagaacac cggcaggatc ccgcagcggc ggaagatggc gatctcgtcg
     3121 tcgtcgaaca tcttgatctt acgccgcagc gtggaaccgt cgttcgactc gtatgtgcgg
     3181 agctcgaaaa ttcgggccgc gcgatttcct tccgacggcg gaacttcaat ccgcggcatc
     3241 gactcgaacc ctcggagaag tgagttctcg atgcgcatat agccgaggtc cggtgcggcg
     3301 ttgtactcct cgcgcgcctt ctcgaacgcg gagtccgcgg ccattttctc catcgccgat
     3361 tccattgccg aaagggaggg aaagctgaca agtgccagaa tgaacgggct gtcgccagcg
     3421 atcacaccac tgaaaaaacc gagcggcccg gcgcccgcgc gcctgagcgc cggaacggct
     3481 gctttttcga ggaaatcgga agtgcgcctg acctggttgg cgtgcgtgtt ccgtagtcgg
     3541 tagttacgaa gttcaaagat cgctttcttg gacgcggcgg aagcggatgg tggtacgaaa
     3601 cccgcgccag ccgcgccggc gaggaagtga cggcggttca tactcaaaga ctttacaaca
     3661 gtggcgcagt cttcaggctg tgaagtgcgt caatgctggt cgataatgct ggtagtgggg
     3721 tggtcgtgcc ggaatcgggc acgtaccgag cgccgattgg cgccacggtg cgtgagtgtg
     3781 gcacgacaat aaggaggcaa agctatggca ctttccaacg acgatcacct tgctactttg
     3841 agcaaggatc tgattcaggc gctcgataat ctcagcgggc tgcatccagg ctttcggccg
     3901 gcgcacgcca aaggcatcat gctttctggc gtcttccatc cagcagccag cgccggctcg
     3961 cttacacgtg cgcctcacgt gcagcggcaa tccacgccag tgacggttcg cttctcggac
     4021 tcgggtggcg tgccgactgt ggcggacaac gacccgaacg tggccggtcc ccgcggaatc
     4081 gcgatccggt ttcatctcgc cgaacacgtc cacacggaca tcatcgggca ctcgaccgat
     4141 ggattcccgg tgcgaaccgc ggaagagttc cttgaattcc ttcgcgcccg ccatgccagc
     4201 ggtcccggcg cgcccaagcc tacgccggtc gagtcctttc tcgccactca tcctgcggcg
     4261 ctgaagttcg tgcaaacgcc caagcccatc cccgccagct tcgcgaaaga atcattcttt
     4321 accgtgacgg cgttcaagtt cacgaacaag gccggcaaca gccgttacgg gcgcttccgc
     4381 atccgtccgg atgggggcgc cgaatacctc gatgcgcagg ccgctgctgc gaaatccgcc
     4441 aatttcctct tcgatgagat cgtcgaacgg ctcgcgaagc atccggttac gtttcacatc
     4501 atggttcaac tggctgtgga cagtgacacg gtggatgatt ccaccgtcca gtggccggac
     4561 gaccgtgcgc agatcgagtt cggcaccatt gtcttaaccg aacggatccc ggaggacgac
     4621 gtcgagaagc gccggattat tttcgacccc attcctcgcg tcgacggaat tgactcatcg
     4681 ggtgatccgc tgctcgacgt gcgggctgcc gtctatctga tgagcgggcg ccgccggcga
     4741 gcggctggtg catcatagga cgctcggaac gacgatcagg gaaggccggc gcgggatcag
     4801 cctgcagccg ccaccggcaa cgcgccgaat cgccgctccc ggttacggaa ctctttcacg
     4861 gccgcggcca ggtcgttcgc ttcgaaatcc ggccagaact tcggcacgaa caggagttcg
     4921 gcgtaggcgg attcccagag taagaagtca ctcaagcgct gctctcccgc cgtgcggatc
     4981 agcagatcca cctccgggac aggacccggc gagtgctccg cctcggccag caggcgggcg
     5041 aacgtctccc gtgtcgtgtc gtgccggcct tgtacccgct gggctgcccg caagatcaca
     5101 tcgcgcgcgg agtagtcaac cgcgacgcgc aggtgcagac gtgtacagcg cgccgtcgat
     5161 cgttcggccg acgcgatcgc cgacgccagc cttgtcccga gccggtcgcg ccgtccgatg
     5221 acgccgatcc gcacgccatt ttcaatacac cggccagtct ccgactgtag gtgctcctcg
     5281 aagagacgca taagggcttc cacttcagcc ctcgggcggc gccagttgtc ggacgaaaaa
     5341 gcatagaggg tcagcgtcgc gattcccagc gcgggcgcgg cctcgacagt gcggcggacg
     5401 gccttcgacc cctccctgtg gcccgccact ctcggaagtc cgcgttccgt cgcccatcgc
     5461 ccgttgccgt ccatgatgat cgcgacgtgc aacgcaggat attgagtact ttgcatctta
     5521 aagtatcctt gcaaaaaaat agagcggtcc gttcagcggc cgcgcgtcgc ctcgatcagg
     5581 gcctccatgt gggcgacgta tctctcaaag gcccggcgtc ccagcgccgt caggcggtac
     5641 tccgtcttcg gcatgcgtcc gtcgaacgac ttcgtgcacg taatgtagcg ggcgtcttcc
     5701 agcttgcgcg cgtggacgct caggttgccg tccgtggtct tcatgagctt cttcagctca
     5761 ctgaaggtga gcgattcgtt cactgccagc gcactgacga tcccgagccg catccgctcg
     5821 tggatcagcc ggtccaatgc gggcggcgcg gcttcggccg ggaactcgcg caacgtacgg
     5881 gagtccccgc tctctctttg tgtctgctcc ttgcggagcg ccgattgctt agccaccatg
     5941 cctccacgca atgaggattc cgaagacgat gtgcagcccg ccgaagccgg ccgcaaggag
     6001 ccagttcccc cacgcggccg gagcgaacag cgctgccgca cccgccgcca tgaagcaaag
     6061 tcccatcgcc gggaccacgc gcaccgaaaa cgcgcccgcg ctcgccacgc cgacgccata
     6121 gagcagcagc cacgttccag gcatcagggc gcttcgcccc gcctgataga gcacgagcgt
     6181 gaagaggccc gccgcgagca tcggcggtgc gaggcccagt ccgaatttcc ttcccggtct
     6241 cgataggagg gacgtgcccg cggccctggc cttatgtgcc atggcaaaaa ccccgagcac
     6301 aaatgccacg aatccttccg ccagccacgt gaccagccac cgctccgtgt ccggttgccg
     6361 cgccgcgatc actgccgcgg cgacggcggt gagccccatc gccacgccgc ctttgccggg
     6421 gaccgccgtg aatgccgccg cgccctccat cgtctcccgg atgaagcgga tgttgtccat
     6481 cgcatgagcg tgtaattcga tcggcaccgc cggctcactc cggatggggc ggacagaacc
     6541 catggcacga ttatcgccgc cgttttgatt tctgtcaagt actttatcgt tcaaagagtg
     6601 atcactccgc gaccctcgcc gtcaccacca gcgtcatcgc gctgttcgag ccgtcgatgt
     6661 tggccttgcc gaccactacc ttctgccctt cgcgcacatc aatcccggtg ttaaaaccca
     6721 cctcggtgaa ctggtaaccg gagccgccgg tggcgtacgg gatccgagcc ccgaacctga
     6781 gctcccggat tcgaagcata cgacccttgt cgtcacccga gacacctacg ctagtgaact
     6841 tggcctgata taaggtcttc gcagagagat tggttggaaa gtcggtggga ggtgcgccta
     6901 caccgctcgc ctctccgctc tccccgtccc gcacgcgcag aaccatcgtg tccagaagtc
     6961 ggaatccctg gtacgagaaa aggttcttca gttgcttcgt caccccctcg agctctggcg
     7021 gaacgcttcc acccggctga gcggaagcca tcagaatgta cgctgtgatc tcgatgttct
     7081 tcggcgccgg ggccggaacg tccatccgtc ggacggcctc ctcgaccgcc gcgaccgttt
     7141 ccggccgtcc cgagaccaca agccggcgca acggcagcga aaactggatc gcggcgccga
     7201 aaggcaacag caagggagga agttcccgca catccaaatt gcgcacttcg atgatcttcg
     7261 tgacctgttc cttctcctgt gccatcgccg gaacaagagt taatgccaga attgccgaca
     7321 aaatcgcgtt tctcattttc aatctccgtt cttatcaatc agccacacaa tgaccacgtc
     7381 cgggtcgtcc gtcagcattt tcaccaccag cggttcaccc ctcggtggcg caggcttccg
     7441 tggtgcgtgc ttcacggcct ttcgcaccgg tcgcggctct attcccggat ctgcgccgcg
     7501 ctgttcagaa gcgtgttcgg caaccggctc acgaacctcc gccaccggag ccggcgccgg
     7561 tggcggtgga acctgccgtt ccggccacaa cgccactccc gccacggcca ccgccgtcag
     7621 ccccactgcc acggcatgcc tccaccgcca tgcgagccac caggaaggcc gcgtgtcttc
     7681 gatcctctcc agcacccgcc tccgcaccgc acccaggacg gcgggatcga tcgcgtcttc
     7741 attcagcgcc ttgagtacgg cctggctccc cagcaactct tcatgcaact cccggcaagc
     7801 cgcacattcc cccaggtgct cttgaacgag cctggcgttg cgcgcatcga gatcgccctc
     7861 tacggaaagg gcgatcagcc tctcgaactt cggacaactc atgttcgtct cctccggaac
     7921 cggtccgcga attccttgat cttcaaccgt gcggaactga cctgcgaccg caccgtcgcc
     7981 gccgtcgagc ctaggattcg tgcgacttct tttgtcggca gcccctccag gtctcgcagc
     8041 acgatcgccg cccgttcctt ctccggcagc cttccgaggg cctcctcgac gatgcgtttc
     8101 tgctcgcggc gctcgtactc ggccgtcggg tcttcgacct gcctctgctc gcccacttcc
     8161 accgcccagt ctcttcgccg ggaacgagcg aggtcccggc agacattcac ggtcacccga
     8221 taaagccacg gtgggaactc acgagtgtcg tcaaacctcc gcaagtgctt gtgcaggcga
     8281 aggaacacct cctgcgcggc atcctcggcg tcctcccggc gccgcagcag gcgcaacgcg
     8341 ataaggaaca cccgccgttc gtgaaggacg atcagttgtt cgaacgcggc cacatcaccc
     8401 gctttggcga gacggagcag gatgacaggc tctgggctct cagatgcctc agcggccgcc
     8461 gtcattgtga gcgccagttc ccgcaccgga ttcggtatcc aaaacagata tactcctccg
     8521 gccggcgaaa cgtgcaaaca gatttttggg aagcggttaa ccagctcgtt tctcgcgttt
     8581 cgtcagggaa tgtcgaccag cagcatctgt tcccggccgt cccgcgtgga gaccagtact
     8641 tcgccctgtg ggtcaacggc gaaggtcggc ccgttgatgc acagcacctc gtgcggccga
     8701 ccgcactgat tcacaaacag cgcat
//
LOCUS       DATIFQ010000254         2228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1613115, whole genome shotgun sequence.
ACCESSION   DATIFQ010000254 DATIFQ010000000
VERSION     DATIFQ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613115"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VLE22_11845"
     CDS             complement(<1..193)
                     /locus_tag="VLE22_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15143.1"
                     /translation="MLAMSSTHRSLTTLLLAVFTVCSAQAPDDNSGFEPIFDGKTLTS
                     WDGEPGFWRVENGAIVGETT"
     gene            complement(247..630)
                     /locus_tag="VLE22_11850"
     CDS             complement(247..630)
                     /locus_tag="VLE22_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15144.1"
                     /translation="MEHVPANDYDTVELRTGRLHKIRRSQARLLGEEDRIVQVVKCYC
                     RSHDVVTAADAELQQRPTFVFPAQFFKNLESLIGRELEGRIYSDSQKRRVKFYLDLKE
                     HAGFQLEENHNDLQSPWYDEGVLKE"
     gene            complement(643..1560)
                     /locus_tag="VLE22_11855"
     CDS             complement(643..1560)
                     /locus_tag="VLE22_11855"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB15145.1"
                     /translation="MKIGITYLYVIFRYGYPHSVDDVLAALPEIRDLGFHYLEMEGLG
                     RAHLLAMQKRRKEVRERLDECGLHVHNFCVVDPDLVSLDAARRKKAIDRFKLGADVAD
                     YLGAETLHLASYAPPVRYVSARPYSLGDKGGYAFADRTLVRIPRGFDWQKVWKALVRS
                     CQACADVAARHRKIVLMEPRVGEVICSVDSMLRLIEHVERPNFKANFDTGHFSAQREN
                     VVLALEKLRGKFANIHISDNDPVNTDHLPVGEGQIDWPEFFRVLKAMNYSGYLGLDLG
                     QSRPLVEGYRTSVETIRSAADALKLKIDL"
     gene            complement(1569..>2228)
                     /locus_tag="VLE22_11860"
     CDS             complement(1569..>2228)
                     /locus_tag="VLE22_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HSB15146.1"
                     /translation="KMLYKTANVQWESRMSGASLGSKILGALKRKLMGESLFWTYFRA
                     DSQGEVGFAGSYPGRIQSFDLAAGQSVIVQRDSFLCAQSSVEVNIEFVKRIGAGFFAG
                     EGFILERLTGPGTVFIHGGGDFVEFNLKPGETLQVDTGCVVGFDASVTYDVQLAGGIK
                     AMMFGGEGLFLTTLTGPGRAIVQSMTLEKMRRELAPARTGGDERSPLESITNIFGSED
                     R"
BASE COUNT          419 a          662 c          718 g          429 t
ORIGIN      
        1 gcgtcgtttc gccgacaatg gccccattct ccactcgcca gaaccccggt tccccatccc
       61 agctcgtcag ggtcttgcca tcgaagatcg gctcgaaccc ggagttatcg tcaggcgcct
      121 gagcagagca gacggtgaag accgccagaa gaagtgtcgt cagtgaccgg tgcgttgaag
      181 acatagcgag catggtccag gatacatcct gagggccgcc acaggctgaa gcctgtgcca
      241 ctggcgttac tctttgagca cgccttcgtc gtaccaaggg ctctgaagat cgttgtgatt
      301 ctcctccaac tggaagccgg cgtgctcctt caggtcgagg tagaacttca cgcggcgctt
      361 ttggctgtcg ctatagatcc gtccctcgag ttccctgcca atcaggctct ccaggttctt
      421 gaagaactgc gcggggaaca cgaacgtcgg gcgctgttgc agctccgcgt ccgcggcggt
      481 gaccacgtcg tgcgaacggc aatagcactt cacgacctga acgatgcgat cctcctcacc
      541 aagcagccgc gcctgggagc gacgaatctt gtgaagacgt ccggtacgca actccacggt
      601 gtcgtagtca ttcgcaggaa cgtgttccat ggtcattcag gttcagagat cgattttcag
      661 tttgagtgcg tcggcggcgg agcgaatggt ctccaccgat gtcctgtagc cttcaacgag
      721 cggccggctc tggccgaggt cgagcccgag atagccgctg taattcatgg ccttcaagac
      781 gcggaagaac tcaggccaat cgatctgtcc ttcgccaacg ggcaggtggt ccgtgttgac
      841 gggatcgttg tcggagatgt ggatgttggc gaatttgccc cgcaactttt cgagcgcgag
      901 cacgacattc tcacgctgcg cggagaagtg gccggtgtca aagttggcct tgaagttggg
      961 ccgctccacg tgctcgatca ggcgaagcat cgaatcgacc gagcagatga cttcgccgac
     1021 gcggggttcc atgagaacga tcttccgatg ccgggccgcg acgtccgcgc aagcctggca
     1081 ggagcgcacg agcgccttcc agaccttttg ccagtcgaag ccgcgcggga tgcggactag
     1141 cgtgcgatcc gcaaaagcgt acccgccctt gtcgccgagg gagtaggggc gggcgctgac
     1201 gtagcggacc ggaggggcat agctggccag gtgcagggtc tcggcgccca ggtagtccgc
     1261 gacgtcggcg ccgagtttaa accggtcgat cgccttcttc cggcgcgcgg cgtctagaga
     1321 aacgaggtcc gggtcgacga cgcagaagtt gtgcacgtgg aggccgcact catcgaggcg
     1381 ctcgcgcact tccttccggc gcttttgcat ggcgagcaga tgcgcgcgtc cgagaccctc
     1441 catttcgagg taatggaagc cgagatcgcg gatctcaggc aacgcagcga gcacgtcgtc
     1501 gacggagtgc gggtagccat agcgaaagat gacgtagagg taggtgattc cgattttcat
     1561 gtgcgtgttt atctgtcttc gctgccgaaa atgttggtga tggattcaag cggactgcgc
     1621 tcgtcgccgc cggtgcgcgc gggagcgagt tcccggcgca tcttctccag cgtcatgctc
     1681 tggacgatgg cgcggccggg gccggtaagc gtggtgagga acagaccctc gccgccgaac
     1741 atcatggcct taatgccgcc ggccaactgg acatcgtagg tgacggaggc gtcgaaaccg
     1801 acgacgcagc cggtatccac ctgtagtgtc tcgccgggtt tgaggttgaa ctcgacgaag
     1861 tcgccgccgc cgtggatgaa gacggtcccc ggaccggtga gccgctcgag aatgaaaccc
     1921 tcgccggcaa agaagccggc gccgatgcgc ttcacgaact cgatgttgac ttcgacgctc
     1981 gactgggcgc agaggaagct gtcgcgctgg acaatgacgc tctggccggc cgccagatca
     2041 aacgattgga tgcggccggg atagctgccg gcgaagccga cctcgccctg gctgtcggcg
     2101 cggaagtacg tccagaacag cgactcgccc atcagtttgc gtttgagcgc gccgaggatc
     2161 ttgctgccga gcgacgcgcc gctcatgcgg ctctcccact ggacgttggc cgtcttgtag
     2221 agcatttt
//
LOCUS       DATIFQ010000255         3901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_658113, whole genome shotgun sequence.
ACCESSION   DATIFQ010000255 DATIFQ010000000
VERSION     DATIFQ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658113"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..553
                     /locus_tag="VLE22_11865"
     CDS             431..553
                     /locus_tag="VLE22_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15147.1"
                     /translation="MPETFKAAGYQTAICGKWHLGHAYAKYLRIAGDSITPTGT"
     gene            557..895
                     /locus_tag="VLE22_11870"
     CDS             557..895
                     /locus_tag="VLE22_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15148.1"
                     /translation="MQSITTATSGREAWTGTSAGVSARNTLHFDGRNLWDAIASNKQV
                     AREDLHFTIESRDGFHFAVRQCEWKLVRHEPRPSGGAVNQLYRLDEDPQETRDVAAHP
                     ALRDGEFSLT"
     gene            900..1175
                     /locus_tag="VLE22_11875"
     CDS             900..1175
                     /locus_tag="VLE22_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15149.1"
                     /translation="MNAKHAHDHTAVGRQETIDLLRGNGHSAAEAVRAFSDEELDRAA
                     PVSLNADAPITAQFLIEDHARSATKPHVTPAMLFPEATCWARSISIW"
     gene            complement(1293..2675)
                     /locus_tag="VLE22_11880"
     CDS             complement(1293..2675)
                     /locus_tag="VLE22_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HSB15150.1"
                     /translation="MDATAVRPGATFKLADPNEGPSRNGFAPVVKKALPAVVKVSSSK
                     VTKTPTEFFGPGQMDPFFRQFFGDDSGGRFNVPREQREQGVGSGVIVSPEGYILTNNH
                     VVDGATDVRVTLSDKREFKASVVGKDAKTDVAVLKIDGNDLPQITIGDSSKVQVGDYA
                     LAIGNPFGVGQTVTMGIVSATGRGGLGIEDYEDFIQTDAPINPGNSGGALVNDRGELI
                     GINTAILSHGSGGNQGIGFAVPVNLARQVMDQILKHGKVTRAYLGLIPQDVTPAMAKA
                     FGVKEHDGALVGDVSPNSPAQRAGVERGDIILEVNGKPVSDANGLRMNISMMAPDSTV
                     NLKVLRNGSTRELTAKLGELEPPKGERASAETPNAGSGLEGVAVENLTPQTARQLGLP
                     ANTPGVVVTNISPASPVADSGLQEGDVIQEIGHKPVKNLSDYQQTMRNAGKEPLLLVN
                     RHGSTFFVVG"
     gene            complement(2906..3556)
                     /locus_tag="VLE22_11885"
     CDS             complement(2906..3556)
                     /locus_tag="VLE22_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB15151.1"
                     /translation="MARLRVLLADDHKLMRSGLRLLLEQQPGFVVVGEADDGLQAVAG
                     VQSLKPDVVVMDIGMPNLNGIEAARKIKGLQPDAAIVMLSMHSDEGYVLRALRAGARG
                     YLLKDSAEGDLVRAVQAVSEGKSYFSPAVGKVLLEDYMRKLQRTGVEDSYDLLSRRER
                     EILQLVAEGKSNKDVANLLNLSPYTVETHRANIMQKLNLRGVPELILYAVRKGIIS"
     gene            complement(3563..>3901)
                     /locus_tag="VLE22_11890"
     CDS             complement(3563..>3901)
                     /locus_tag="VLE22_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB15152.1"
                     /translation="QMEVDVQSEDISEDLPDEYKTCIYRLVQEALNNAAHHATARSAH
                     VTARQTAEKIIVAVTDDGRGFDPQKTRGMGILGMHERVKRLSGKLTIESQPGHGTVLT
                     AELPLSAGGR"
BASE COUNT          721 a         1255 c         1129 g          796 t
ORIGIN      
        1 gtccgcattg ggattcgcat tcaggatgat cacggttcta ttcccgtcag cgggcgactg
       61 gaatgcgtac agatcgcaga ggtcgaggcg ttgatcgccc agcggcgggc ctagactgag
      121 cccagtgaaa tggtttgaca tagcccctct cctttctatt cattatattc gacattcgcg
      181 gaaccggctt ttcccagcct gccctcgatc gtccgaagcg ctcgggaaac ccgcccggtg
      241 gctccccgcc tgccgccggt gcctgagcgc ctgcgccgac cattcggtcg aggacgctcc
      301 ggcatcgtgg ttgcttcgag tgcgtggtca agaccggcgc gatccgcgct gatgacggga
      361 cgatcgccga tgcggctcgg ggtggcgtac agcgtggtcg atgtacgggg tcccggtgag
      421 cgagcacttc atgcccgaaa cattcaaggc cgctgggtat cagacggcga tctgtggcaa
      481 gtggcatctg ggacatgcgt acgcgaagta cctccgcatc gcaggggatt cgatcacgcc
      541 tacgggcact tgaacggtgc aatcgattac tacagccaca tccgggaggg aggcctggac
      601 tggcacctcg gcgggcgtat cggcgcgtaa cacactccac ttcgatggcc gcaatctatg
      661 ggatgccatc gccagcaaca aacaggtggc gcgggaagac ctgcacttca ccatcgagtc
      721 tcgggatggc tttcacttcg cggtgcgcca gtgcgaatgg aagctcgtgc gtcatgagcc
      781 gcggccgtcc ggcggagcgg tcaatcaact gtaccgtctc gacgaggatc ctcaggaaac
      841 cagggacgtg gcggctcacc cggcactacg ggatggcgag ttctcgctca cgtagatagt
      901 tgaatgcgaa acacgcgcac gaccataccg cagtcgggag gcaggaaacc atcgatctcc
      961 tgcgcggcaa cggccattcc gccgcggagg cggtgcgcgc cttctccgac gaagagctcg
     1021 accgggcggc gcccgtctcg ctgaacgcag acgccccgat cacggcgcaa ttcctcatcg
     1081 aggaccacgc gcgctcggca acgaagcccc acgtcactcc cgcaatgttg ttcccggagg
     1141 cgacctgctg ggcgagatcg atctcgatct ggtagacgct cgccacatgg tgaatgatca
     1201 cgccgacctg tagggcagcc agcatgttac aaagctgagc cagccccagt cctcagggga
     1261 actggggccg gcgtcctcag ggagttgatt tctcaaccca ccacaaagaa ggtgctgccg
     1321 tgacggttca ccagaagcag cggttctttc ccagcgttgc gcatggtttg ctggtagtcc
     1381 gacaggttct tgacgggctt gtggcctatt tcctggatca catcgccctc ctgcaagccg
     1441 gagtcagcga ctggactcgc cggactgata ttggtcacaa ccacacccgg agtgttcgcc
     1501 ggcaaaccga gttggcgcgc agtttgtggc gtcaggtttt ctacagcgac cccttcgagc
     1561 ccactcccag cattcggcgt ctcggcactc gcccgctcac ccttcggcgg ttccaactcg
     1621 ccgagcttcg ccgtaagctc acgtgtgctg ccgttgcgca gaaccttgag gttgacggtc
     1681 gagtcaggcg ccatcatcga gatgttcatc cgtaaaccat tagcgtccga tactggtttg
     1741 ccgttgactt cgaggatgat atcgcccctc tcgacgcccg ctctctgagc cgggctgttc
     1801 gggctcacgt cccctacgag cgcgccatcg tgctccttga cgccgaacgc cttggccatt
     1861 gcaggggtga catcctgcgg gatgagcccc aggtaagcgc gcgtaacctt gccgtgcttc
     1921 aggatctgat ccatgacctg ccgcgccaga ttgacgggca ccgcgaagcc aattccctgg
     1981 tttccaccgg agccgtgaga gagaatcgcg gtgttgatgc caatcaactc accgcggtcg
     2041 ttgaccagtg ccccaccgga attgccgggg ttaatgggcg cgtcggtctg aatgaaatct
     2101 tcgtagtcct cgatgccgag gccgccgcgg ccggtcgcgc tcactatccc catggttacg
     2161 gtctgtccca ccccaaaagg gttgccaata gccagcgcgt aatcgccgac ctggaccttc
     2221 gaggagtcac caatggtgat ctgtggcaga tcgttgccgt cgatcttgag tactgcaacg
     2281 tcggtcttgg catccttgcc tacgacactc gccttgaatt cacgcttgtc ggaaagcgtg
     2341 acccggacat ctgttgcgcc gtccacgacg tggttattcg tcaagatgta gccttcgggg
     2401 ctgacgatga cacccgaacc cacaccttgt tcacgctgct cacgagggac gttgaatcgg
     2461 ccgccagagt cgtcaccgaa aaactgccgg aaaaacgggt ccatctggcc cggtccaaag
     2521 aactcggtcg gcgtcttcgt gaccttcgag gaagaaactt tgaccacggc cggcagcgcc
     2581 ttcttgacca ccggggcaaa accgtttctg ctcggacctt cattgggatc cgccagttta
     2641 aaggttgcac ccggtctcac cgcggtggcg tccatggccg cacgaaccgc cccgagtccc
     2701 ccaaccgcca atgccaagcc cacgcccagg gccaagccct tagaacgtgt ccgacttatc
     2761 catttcctca tgttgcctcc cttatccaag tgacgtattt ccaaggccct atggccctcg
     2821 tcactcatat ggacgtaccg gcggccggct cggcccatcg cgtcgattgg gtagtgcggc
     2881 tcccggcgac tgagtagtcg cgccttcagg agatgatgcc cttgcggacc gcgtaaagga
     2941 taagttccgg cactccccgg aggttcaatt tctgcatgat gttcgcccgg tgagtctcca
     3001 cggtgtaagg actgagattg agaaggttgg caacatcttt gttggacttg ccctccgcaa
     3061 cgagctgcag gatctcccgt tctcgccggg agagcaagtc ataggagtcc tcgacgccgg
     3121 ttcgctggag tttacgcatg taatcctcca gcagcacctt gcccaccgcc gggctaaagt
     3181 aggacttccc ttcggaaacg gcctgaacgg cccttacgag atctccctcg gccgagtcct
     3241 taagtaggta gccgcgcgcg ccggctcgca gagcgcggag cacgtacccc tcgtctgagt
     3301 gcatgctcaa catcacgatg gccgcatccg gctgcagccc tttgatcttc cgggcggctt
     3361 cgatcccgtt gaggttcggc attccgatgt ccatcaccac tacatcgggc ttcagagact
     3421 gcaccccagc cacagcctgg aggccgtcgt ccgcctcgcc caccaccaca aaccccggtt
     3481 gctgctcaag caggagacgc aggccggacc gcatcaactt gtgatcgtcc gccagcaaca
     3541 cgcgcagcct tgccatcgtc gcctatcgcc cacccgcact tagcggcagt tccgccgtca
     3601 gcacggtccc atgtccgggc tgcgactcga tggtgagctt cccgctgagc cgtttgaccc
     3661 gctcgtgcat tcccaggatg cccatgccac gagttttttg cggatcgaat ccacgcccat
     3721 cgtctgtaac ggctacgatg atcttctcgg ccgtttggcg agcagtgacg tgagcgctgc
     3781 gagctgtagc gtggtgcgcg gcattgttta gggcttcctg gaccaagcgg tagatgcacg
     3841 tcttgtactc gtctggcaag tcttccgaga tgtcctctga ctgtacatcc acttccattt
     3901 g
//
LOCUS       DATIFQ010000256        24528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_466366, whole genome shotgun sequence.
ACCESSION   DATIFQ010000256 DATIFQ010000000
VERSION     DATIFQ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24528
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466366"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1511)
                     /locus_tag="VLE22_11895"
     CDS             complement(96..1511)
                     /locus_tag="VLE22_11895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB15153.1"
                     /translation="MQESSLSVLVPLYNEEEFIATLLERVLSAPLPDGMTREVIVVDD
                     GSRDGSAEAVEAFVTRFPDIVRLVRHDRNRGKGAAVRTAIENARGEFVVIQDADLEYD
                     PGEYQTLLKPLVEGKADAVYGSRFLVAGERRVLYFWHALANRILTTLCNMVADLNLTD
                     METCYKACRTSLLRSIPIRSDRFGIEPELTMKLAQREARIYETPISYYGRTYEEGKKI
                     GFRDAVAAVGVIFRYWLVTDIYKEAGPEILETLSGAPRFNRWMADVVRPYVGRRVLEI
                     GAGIGNLTRRLAPRRERYIATDIDEEHLARLRTRLQHRPNLEIRRCDLQASQDFASIQ
                     ETVDTVVCLNVLEHIEDDLAGLRNIHAALMPGGRAIILVPHGQEIYGTLDKALGHFRR
                     YRHDELRVRMEQSGFQVEQILDFNRASRPGWYVNGKLLRRATISPFQLKLFDRLVWLW
                     RRVDPLLPWGPTSIIAVGRKP"
     gene            complement(1536..2390)
                     /gene="purU"
                     /locus_tag="VLE22_11900"
     CDS             complement(1536..2390)
                     /gene="purU"
                     /locus_tag="VLE22_11900"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HSB15154.1"
                     /translation="MRNTAILLVDCPDRKGIVATVADFLYSHGANILHADQHQDNEIQ
                     MFFTRIEWALDEFDLDEPAFHEQFQPIAEGFHMHWRLEYSSASMGVAIFVSRELHCLA
                     DLLYRRGAGELRCRIPMIISNHPDARPLSEFHGISFHHIPIDPAHKREAEDGQLRLLE
                     DSGVSVIVLARYMQILSPEFVARYPGRIINVHHSFLPAFSGARPYHAAYQRGVKLIGA
                     TSHYVTDALDEGPIIEQDVTRVSHRCELEDLIEKGRDLERVVLSRAVRWHLEHRILCY
                     ANKTVVFD"
     gene            2428..3291
                     /locus_tag="VLE22_11905"
     CDS             2428..3291
                     /locus_tag="VLE22_11905"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB15155.1"
                     /translation="MRLLILTACLPLVTPLLAADRPVDPTWLHKFLPDVPAAAVDAAG
                     TGCRYKAVFGEGDSNARVLRGIVRFGEWLVDSGASCTPVVLPREEQIYVVLEGGGTVH
                     YGGERVSVEKDDYMYVPPGVRGKVSSSSGAVLRMLVMGYKIPDDIKISLPPGPQKANI
                     NEVKKQTVGGHPPSVLYQLLMGDTTSTRDRIAAAHVMTSLFTMVFEPGGTNAPHHHET
                     EEEIYLVLDGEGEMVAGGGMNGVEGRHPVKPGDAFFFRLNATVGFYSSTAPGNKARIL
                     AVRSRYPFGRR"
     gene            3362..4132
                     /locus_tag="VLE22_11910"
     CDS             3362..4132
                     /locus_tag="VLE22_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HSB15156.1"
                     /translation="MRSIARWLLLTALLTLDFPACAWGPTGHRAVGRFTEKYLTAKAA
                     RAVKDLLGPDSLAEAGTWADEIRSDPAWKRAYTWHFVSIEDDETYETAKKEPAGDILV
                     KLHHFESVLRNAKATRDDRVIALKFFVHLIADIHQPLHVGRRDDRGGNNIAVNWHGRP
                     ESLHRVWDSLMIDGERLSFTELAEFLDPPSAQQISTWQKTGYLDWARESCELRGRVYD
                     IGDGQLGYDYSYKNFPVVEERLRQAAIRLAGKLNSIFK"
     gene            complement(4149..5516)
                     /locus_tag="VLE22_11915"
     CDS             complement(4149..5516)
                     /locus_tag="VLE22_11915"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB15157.1"
                     /translation="MPRTVLVVDDEPVARYAIVRAFHAEHRVVEASSVAEAREKVLSD
                     RPDVILLDYNMPEEDGMVLLRELQAEPESPAVIMITAHGSERLAVDAMKAGAYDYLAK
                     PYDLDELRLLLARALERQQLRNEVHALRERLAAEGQFGRMIGASTAMRELFVLAERVA
                     QTELPVLLLGESGTGKDLLAQEIHARSNRAGQRFVALNCAAVPETLVESELFGYVKGA
                     FTGATASRPGRFELAQGGTLFLDEIGDMAPATQAKILRAAESGVVERLGDSKEVRVDV
                     RIISATNQDLDKAIQEGRFRQDLYYRLAGVTLYLPPLRQRREDIPLLVEQFWAECSHR
                     YERPGPELSREAMVSLQQAPWRGNVRQLRNAVERLFVLARGERVIEDDVSAATQSGPP
                     ASPLEDDLLAPPDFREARQQFEAAYLSRKLRENQGNVTRTAAAIGLERQSLQEKIKKL
                     GIERE"
     gene            complement(5579..6076)
                     /locus_tag="VLE22_11920"
     CDS             complement(5579..6076)
                     /locus_tag="VLE22_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15158.1"
                     /translation="MKTILLVMILATVIAGPAGAQTEKDFSGQGYAFFAPGVTSPDGS
                     GFVHYGAGAEGLVYKGLSAGAEIGYAHPWRGFRQGLGVFSVDGGYFFRNARSASAKFV
                     PFVNGGYTLLFRSGSESGFNVGGGVISWFSERVGLRLEVRDQVFSDGGSLAHLVGFRI
                     GLAFR"
     gene            6232..9051
                     /locus_tag="VLE22_11925"
     CDS             6232..9051
                     /locus_tag="VLE22_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15159.1"
                     /translation="MGARNRWVLVVTCLLVVPPWAQGAGVTVRFDPLSPEVGPFPADS
                     LTVADATQKTGVRVNLPLSNCAAQPSTCEDLKLINELDGFNLQPRVRVRFSGPVNTAT
                     LRSGIFVVALDDIVASVLGVHLAGQVIAINQVIFDPDTNTAYGEADEVLDQHRRYALV
                     VTDAVRDASGDPVEQDPTYGRCLAPLFQSAYCRQLSEVVKTLRVPGKIVAASRFTTLS
                     ATSWLEKARARIQGSTVNVRRPSPKNTFKVASLLAFSLRLQVKSNPPAFEDFTVPQPL
                     LAAILADVDRIAFASFDSPRFLDAKQTIPAVPTAGEVALPSATTGVQFHAFLPKGDKP
                     KTGYPVVIFGHGLNDSRFGAPTLVANTFARNGFATIAMNAVGHGSGPESTVSLLELSG
                     STTLTAGGRSIDLNGDGSIESREGCLLPGPGPFGLRDCLRQTALDLAQFVRVLRAGLD
                     VDGDGSVDLDPGRIYYAGQSLGALYGTVFNAIEPEIRVTALNVGGATVMDVARWSPSF
                     HSVTRDFLGTRMPPLLNAGGDFNENYVLRGEPAKTNDVTGAINIQNLFETSEWLQMSG
                     DPIAYAAHLKLAPLAGATPKRVLWQFARGDRTVPNPQNSALIRAAGMREESRLYRHDL
                     ARAVAPDLSENPHAFLVDIQSAPGFAIAAAAQQQIAGFFAADGMQIPDVNKLVPLLFG
                     RNVFETPASLPEDFGFGASVVSVSAASYDFVVAPESIVAAGGTGLAVRTEAATSRSLP
                     TAIAGTTVRVVDSTGVERLAPLFFVSPQQVNYILPAGTTPGMARVTVTSGDGEASSGT
                     AQVESVAPALFTANASGSGVAAAVATLAKVDGSQSSDVVFQCLAGPGTCVAKPIDLGA
                     AGDRVYLLLFGTGIRGRSGLTAVRLTLDGANVPVDYAGAQSEFAGLDQVNAGPIPRSF
                     TGRGETAVELTVDGKPTNIVTISFR"
     gene            complement(9074..10414)
                     /locus_tag="VLE22_11930"
     CDS             complement(9074..10414)
                     /locus_tag="VLE22_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15160.1"
                     /translation="MNRNSDNLSRRSFLKNSSVIGAGTSAFTIIRPELVRGAGKEKLK
                     AGLVGCGGRGTEAVVNLLTGTENVEVVAMGDIFEDRLEKSLASIRDETKNPKLQDRVK
                     VPPDRHFVGFDAYKKVIASDVDIVMLATPPGYRPEHFEACIAAKKHVFCEKPFGTDPV
                     GVRRFMAAAKKSEELKLTVMSGAQRRFARDYTETIDKIKNGAIGNVVATYAYWVGGPV
                     LQFLGGGWPGPKGQRDPKWSDMIWQNRNWYSFVWLCGDQVVEQHLHNIDVCNWVMGTH
                     PEKVVASGGAIWRPREAAYGNIYDHLSADFEYANGVHMSSYCRQYPKGCATNVSELIV
                     GAKGRSNAHDMGSGSGQDVRGGFRDPMENPYVREHTAMVNSIRGDGPYINHAMAVAES
                     TMTCIMARESAYSGLEVTWDMIMNSKRDLSPKAMSYEANIEVPPLPVPGEYKFI"
     gene            10560..11462
                     /locus_tag="VLE22_11935"
     CDS             10560..11462
                     /locus_tag="VLE22_11935"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HSB15161.1"
                     /translation="MIQGVNVAAVTPRRVNADGVDLGATLELIDFLCDKGAGGIALLG
                     STGEFSHFDLEQRLHLIQFGVKRSRAPVLVNVSHSYLDGTLALARGAASYGAAALLLM
                     PPIFFRYPQEDIRTFYQEFARDLGVSVPVFLYNVPFFTSEIACETAEGLLAEGHFAGI
                     KDSSGEIEYVQRMISLKQQRPLTVLVGNDKIFTAARSAGADGVVSGVGCALPELMVGL
                     DRAILSGDTTQRDRLELRLQEFIDWIDSFPVPIGVREAVALRGLKTGPHATPLGEGTQ
                     RRLGEFREWFQGWLPGMLKEASRA"
     gene            11455..13104
                     /locus_tag="VLE22_11940"
     CDS             11455..13104
                     /locus_tag="VLE22_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSB15162.1"
                     /translation="MRKGLLILLLGTALSAQVLVPGPQAAVFLSGVDDSDQPYGLYVP
                     KNYDASKKYPLVISLHGAWSNHRVNLRRVFGKGNRPGESDAEATRYFPPLRDVDFIVA
                     SPFARGTMGFMGIAERDVYDVLADVKKRFSIDEDRIYLTGLSMGGGGTLWLALSRPDI
                     WAAIAPVCPSIAQGTQELAGNAFHLPVKLFHGDIDTAVPVDVSRTWHKLLLGLGVKAE
                     YVEYPGVKHNSWDNAYKDGAIFDWFAQFRRNRFPDRVRFATRFYKYSSAYWVRIDGLT
                     TGELATIDAKFTDKNRIEIDTAQVDGFTLQLAGHPMFESGRPVSVKLNGAELAAQGAE
                     AVSFRSSRGVWQAGQYAQPTGGKRTGVEGPIREVVSGRHLYIYGTIDSPGSGELEQRR
                     TVSSFASDWQGPSARLALWLRSVADKEATDADLYGANLVLFGTKETNALIARYSTFFP
                     LELNAGAADYGLVFIAPVGDRLVLVNSGLPWWTGADRAKRPGLRYLWSPYQVLLSFGD
                     FILFKGSLENVIAEGNFTRSWKVPPEAAEKMKATGAVKVLR"
     gene            13101..13223
                     /locus_tag="VLE22_11945"
     CDS             13101..13223
                     /locus_tag="VLE22_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15163.1"
                     /translation="MNNTIGQFGHAGHELARWIADYRLSFTTPERSEATMIEFA"
     gene            13248..13934
                     /locus_tag="VLE22_11950"
     CDS             13248..13934
                     /locus_tag="VLE22_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15164.1"
                     /translation="MFQELLPLLAALAIAAIVYTVREVHRRRFTVRPPLPDLTLREGW
                     ELWRRGGAQHPDIKFFPADAALTDGVAAEMERALPAAEAGISGADHPRQAVRRSILEN
                     AGVALHLEAIIGLGQEERKALLKGYREGMEGLLQNAWRLSVHRWILLRFYGRLKFDDA
                     VPDDWFQHYMRVARPYIREKVRLAREHVLERNEGSGQFAQVYDQLLHQLAEDTLKIRP
                     KRRFVAPDLS"
     gene            complement(13965..15122)
                     /locus_tag="VLE22_11955"
     CDS             complement(13965..15122)
                     /locus_tag="VLE22_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15165.1"
                     /translation="MNSLRWYTKALLIIAFVAPAGSQISPRIFEPPDGAILNRHDGET
                     TPEGLRITVKGLAGSAGPLRINGISVPVKEGAFETPIVLRDRETKIVAETSGGEKDSI
                     TVLWDRDSFPRYRFSTDDNIWFLRDIARNAERYRSIFENPYLALWQDMHRKYGTKVHF
                     NIYYETEDFNLSQMPDKYRDEWQRNRDWIRLTFHARSNKPDRPYIRASGEQIRYDYRA
                     VTREIERFAGRELLSPVTTVHWGETTREGARALRDEGIRIMPGYFRMQDGRPSVSYYL
                     SRPEVLYLSGRDYWKDNSEDIIFFRHDIVVNTVALNDIEPYLEKVAADPHQSEVIELM
                     IHEQYFYPDYVAYEPDYRDRVERAIRWVTNRGYKPVFFGDGFLGTGKDGGK"
     gene            complement(15179..16024)
                     /locus_tag="VLE22_11960"
     CDS             complement(15179..16024)
                     /locus_tag="VLE22_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15166.1"
                     /translation="MLLIAGKRVARRSRGERIVLSAALSLLGWLPGARAAVGTTRIEP
                     HTEAEFQAYVKAREPALERRAQGSSFLWCAERAERLTALRRSGVVIEPARERAVTRIN
                     DGLVHDWIGATFIPGATLAQTLAFLQNYDNHKNVYAPEVADSKLLGHDGDRYRVYLRL
                     RKHKIVTVVLNTEYDVNYRRLSARRAWSRSYSTRIAEVEDPGTSSEKEMPPGHDHGFL
                     WRLNSYWRFEERDGGTYVECEAISLSRDVPMLLSGLIMPIVKELPEESLRKTLVATRN
                     ALTAR"
     gene            16136..17242
                     /locus_tag="VLE22_11965"
     CDS             16136..17242
                     /locus_tag="VLE22_11965"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB15167.1"
                     /translation="MDFRIFRHLPALTLAAFLFVLGPARPACAQDSPEKILLKDYRPK
                     SIYKIPVTQIAKAKYWVIDMHSHDYAKTPEQIAEWVKNMDEVGIQKTVLLTGAVGPRF
                     DEIHKQYSKYPDRFELWCGFDYTGYNEPGYPASAVKELERCYRAGARGVGEIIDKGKG
                     LGWGKNRADGMHPDDARMDPLLEKCAELGMPVNIHVADPIWMYEKMDNTNDGLMNAYE
                     WRLDNQPGIVGHSGMIDIIERAVKKHAKTKFIVCHFANLCYDLTRLGGMFDRNPNLYA
                     DISARYAETATIPRFVSKFYAKYVGRLVYGTDMGYNTSMYRITFRVLESLDEHFYESE
                     QFGYHWSMNGFGLPDKVLKQVYRDNALKILKAGR"
     gene            17267..18682
                     /locus_tag="VLE22_11970"
     CDS             17267..18682
                     /locus_tag="VLE22_11970"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HSB15168.1"
                     /translation="MIAPLCVAAQLAMGAGPISVVEYDTSTEDFPNPERGFYAQLLLS
                     DRRPGSSSLSAAQLESLRLKNQTVIRRLYEISSFRDGDLSPAFLDTLRHDFDTARKSG
                     FKLILRFAYDFTAKGQDAPKNVILRHLDQLKPVFEADADVIAFLEAGFVGAWGEWHSS
                     SHGLDRDSDAKREIVLKQLEVLPKSRMTSLRTARYREEVFGSEAVRPLTLDDVLHVVP
                     RARVGYYNDCFLGSDTDVGTYLGEDEAARAVEKDFIARETRFVVQGGETCAVSPPRSD
                     CATALAELAQMHWSYLNSSYHPGVLKGWRAQGCMEEISRRLGYRFRLVRSSMTGTAAQ
                     GGAFEMSFEISNDGFASPHNPRLVELVLREPDRGGEYILALEEDPRMWASGETRNVSV
                     RGGIPRDVPPGRYQVFLNLPDPAPSLYGKSEYSIRLANSALWQPETGYNSLRQRVEIQ
                     SAAGKRYAGRNWFVARKPAVK"
     gene            complement(18692..19828)
                     /locus_tag="VLE22_11975"
     CDS             complement(18692..19828)
                     /locus_tag="VLE22_11975"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HSB15169.1"
                     /translation="MTARQLQSIARKNGTPVVVIDHEMIRRNYARFRRNLPRVQAYYA
                     VKANPAPEIVRTLYNVGASFDVASLPEFMLVHENIRRLPAKEQQDFIWDKIVYANPIK
                     PKETLEALNRYKPLVTYDNLTELRKIRKYAPQAGLALRLRVPNTGSMVELSSKFGCDP
                     GEAVDLIDEAFRAGLVVEAISFHVGSQCTNFENFVQALNMSAAVLREAKTRGHEIKIL
                     DIGGGFPAPYDRHVRPFSELARKINAEINRLFPADMQILAEPGRFLVAIAATSVARII
                     GKAVRDGKTCYYIDDSVYHTFSGIIFDHCQYHIKAFRKGDTEICAVFGQTCDGLDCIS
                     QSDELPDLEIEDLVYSENIGAYSNASATWFNGFPPAQVVHVNES"
     gene            complement(19859..21070)
                     /gene="speY"
                     /locus_tag="VLE22_11980"
     CDS             complement(19859..21070)
                     /gene="speY"
                     /locus_tag="VLE22_11980"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HSB15170.1"
                     /translation="MAKTEKTHRRYREDLSEMPDWLKKKTCPNRDKYLSGKRILPRNL
                     TGREKLAAIVDDTFLAYNAARLKESCQLFVEKMLEPDVTVGMSLSGALTPAGLGCSSV
                     VPLIKAGFVDWIVATGANLYHDLHFALNYPVRVGSFKMDDTELRNNDIVRVYDVLLGY
                     SDCLMATDEILRDILVQPEFQKEMGTAELHYLLGRYAAEWERKAGLKDVSVLAAAYRA
                     GVPCYTSSPGDSTIGMNVAGVELRGNKLRINPSIDVNETTAFVLAAKRAGGKSAVVLW
                     GGGSPKNFMLQTEPQIQEVLRIKEFGQDYFLQVTDARPDTGGLSGATPSEAVSWGKVD
                     PDRLPDAVVCYTDTTIAMPILTHYALAKHKPRKLRRLYGQREMMIKALTKEYFRHTKA
                     KMVDGSEPVLA"
     gene            21087..22181
                     /locus_tag="VLE22_11985"
     CDS             21087..22181
                     /locus_tag="VLE22_11985"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HSB15171.1"
                     /translation="MAEPRTATSQTPAALGYSMPAEWDPHEATWLGWPHNPTDWPDKL
                     DTVRWVYGEIVRKISAGELVRVLVNSKTEEKLARRYLTRAGADTGRVEFIVHPTNRGW
                     TRDSGPVFVRRRTGKRAETAIVHFYFNAWAKYPDWQKDRRVPETAAKRLGKRLFHAEC
                     SGRRFVLEGGGIDVNGRGTLLTTEECYLDPVTQVRNPDLGRSEIEETLKAYLGVTNVF
                     WLGGGVVGDDTHGHVDDICRFVNANTVVLIREDNPKDINFRPLAENWERIKDMRLEDG
                     TRPEVVALPMPGPIFFDGDRLPASYANFYIANAAVIVPTFNDPKDRVALGILGELFRD
                     RPVVGIHAVDLVLGFGTLHCLTQQQPLAAV"
     gene            22195..23487
                     /locus_tag="VLE22_11990"
     CDS             22195..23487
                     /locus_tag="VLE22_11990"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: protein motif:HMM:NF019625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-tagatose-bisphosphate aldolase, class II,
                     non-catalytic subunit"
                     /protein_id="HSB15172.1"
                     /translation="MTDDIISRIVTENRRGRAAGICSVCSAHPWVLEATLAQTLAAGG
                     PALIESTSNQVNQFGGYTGMTPARFAGDVRRLAERMGFPEGRLLLGGDHLGPYPWRKQ
                     AASEAMGHARAMVRDYVLAGYVKIHLDASMPCADDASPVLDPAVAAWRAAELCRAAED
                     AWRELPASSPAPVYVIGTEVPVPGGEQLESGAPAVTKPEDAKATVEMHREAFAALGLD
                     GAFERVIGLVVQPGVEFGSAIVFGYDRGRAAVLSAQLPDRPELVYEAHSTDYQTAEAL
                     REMVEDHFAILKVGPWLTFAMREAIFALSMVEQEWLSGGVRLSRVREALEEAMLKNPA
                     HWKDYYQGSEDELRLARKYSYSDRCRYYWPDPAVQAEVDTLVRNLTQHPPPLTLISQF
                     LPAQYDAVRNGTLSHDSASLIKDRIREVLRLYASACGE"
     gene            23645..>24528
                     /locus_tag="VLE22_11995"
     CDS             23645..>24528
                     /locus_tag="VLE22_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15173.1"
                     /translation="MSFALSSAPPRRLVALFLALTLAPAAGLVWLGWRLLEQDRALEA
                     QRAQERREYAADLAASALSRKLSLARQRLGQPERAADLASAHDSLVVVFRQQQVEAFP
                     AGRLLFYPFVLPEGMAAENAGQQFRTGEEYEFRHKDFAKAIENFRSLVFSRDLVARAG
                     AHLRLARNYRKSGDLELALAEYGELTRAERVVIDGVPAELVARGSRCALLAELGRSDE
                     LLREARVLYEDMLRGRWRLTRPVFQLYASQARGWLRRSDDGERGQLALSDTVERLWGQ
                     WQAARAGDRPMAGATVLRV"
BASE COUNT         4621 a         7376 c         7760 g         4771 t
ORIGIN      
        1 cggtcgaagg ggccagcagt tcccggtcaa gtgggttcgc ctgacaccgc gcttcactcc
       61 atagccgagc tgctggaggc gcaattccgc tggcgctacg gtttcctgcc cacggcgatg
      121 atcgaggtgg ggccccaggg cagcagcgga tccacgcggc gccacagcca gacgagcctg
      181 tcgaacagct tcaattgaaa cgggctgatc gtggccctcc gcagcagctt tccgttgaca
      241 taccagccgg gccgggaagc gcggttgaaa tccagaatct gttccacttg gaagccggac
      301 tgttccatcc gcacgcgcag ttcgtcgtgc cggtagcgtc gaaaatggcc gagggccttg
      361 tcgagcgtgc cgtagatttc ctggccgtgc ggcacgagga taatggcgcg tccgccgggc
      421 atcagggcgg cgtgaatgtt ccgcaggccg gcaaggtcgt cttcgatgtg ctcgagcacg
      481 tttaagcaga cgaccgtgtc gacggtttcc tgaatggagg caaagtcctg cgaggcctgt
      541 aggtcacagc ggcggatctc cagattgggc cggtgctgga gccgtgtgcg cagacgagca
      601 aggtgttcct cgtcgatgtc agtggctatg taacgctcgc ggcgcggcgc gaggcgccgg
      661 gtgagattcc caatgccggc gccgatttcc agtacccggc ggccaacgta aggccgaact
      721 acatcagcca tccagcggtt gaacctgggc gctccggaaa gcgtctcgag gatctcgggg
      781 cctgcctcct tgtagatgtc ggtgacgagc cagtagcgaa agatgacgcc gacggccgcc
      841 acggcgtccc ggaagccgat cttctttccc tcctcgtacg ttcggccgta gtagctgatg
      901 ggcgtctcgt agatccgcgc ctcgcgctga gcgagcttca tcgtgagctc cggctcgatg
      961 ccgaagcgat cgctgcggat gggaatactg cgcagaaggg acgtacggca cgctttgtag
     1021 caggtctcca tgtcggtcag attaaggtcc gccaccatgt tgcacaacgt cgtgagaatc
     1081 cggttggcga gcgcgtgcca gaaatagagg acgcggcgct cgccggcaac gagaaaccgt
     1141 gagccataga cagcatcggc cttgccctcg acgaggggct ttaggagcgt ctggtattca
     1201 ccggggtcgt actcgagatc cgcgtcctgg atgaccacga actctccgcg ggcgttctcg
     1261 atggctgtgc ggacggccgc tccttttccg cggttcctgt catgacggac aaggcgcacg
     1321 atgtccggga atcgcgtgac gaaggcttcc actgcttccg ccgagccgtc acgggaaccg
     1381 tcgtcgacaa cgatcacctc tcgggtcatt ccatcgggga ggggagccga gagcacccgt
     1441 tcgagcaggg tggcaatgaa ttcttcctcg ttatacagcg gtacaaggac cgagagcgaa
     1501 gattcctgca tggaattccg ctattgtaac gccgttcagt cgaacaccac cgtcttgttc
     1561 gcgtagcaga ggatacggtg ttcgaggtgc cagcgtacgg cgcgagagag gacgacccgc
     1621 tccaggtcac ggcctttttc gatgaggtct tcgagttcgc aacggtgaga gacgcgagtg
     1681 acgtcctgct cgatgatcgg gccctcgtcg agcgcatcgg tgacgtagtg actggttgcg
     1741 ccgatgagct tcacaccgcg ctggtatgcg gcgtggtagg gacgggctcc gctgaaggcc
     1801 ggcagaaagg aatggtgaac gttgatgatg cgcccgggat agcgggcgac gaactccggc
     1861 gagagaatct gcatgtagcg ggcgagcacg atcacgctga caccggaatc ttccagcagc
     1921 cgcaactggc cgtcctcggc ctcacgtttg tgagccggat caatcgggat gtgatggaac
     1981 gagatgccat gaaattccga gagcggacgc gcgtccgggt ggttgctgat tatcatcgga
     2041 atgcggcaac gaagttctcc ggccccgcgg cggtaaagga ggtcggctag acagtgcagc
     2101 tcgcgcgaga cgaaaatcgc cacacccatg gatgccgaag agtactccag gcgccagtgc
     2161 atgtgaaacc cttcggcgat cggctggaac tgctcatgaa acgccggttc gtcgagatcg
     2221 aactcgtcga gggcccactc gatgcgcgtg aagaacatct gaatctcgtt gtcctggtgc
     2281 tggtccgcgt gcaggatgtt ggcgccgtgg gaatagagaa agtcggcgac cgttgcgacg
     2341 atgcccttgc ggtcggggca gtctacgaga agaatcgcgg tgtttcgcat ggacgggtat
     2401 atgagtatac tgccatggga tacctgaatg cgtttactta tactgaccgc ctgcttgccg
     2461 ctagtgaccc cgctcctggc ggccgaccga ccggtggatc cgacctggct tcacaagttc
     2521 ttgcctgacg ttccggcggc ggcggtggac gccgcgggga caggctgccg ctacaaggct
     2581 gtctttggcg agggtgattc taacgcacgg gttctgcgcg gcatcgtgag attcggcgag
     2641 tggcttgtgg actcgggcgc aagctgtaca ccggtggtgc tgccacggga agaacagatc
     2701 tacgttgtgc tcgaaggcgg aggaaccgtt cactacggcg gcgagcgcgt gtccgttgag
     2761 aaggacgatt acatgtacgt tccgccaggc gtacgcggca aggtgtctag ctcatcgggc
     2821 gcggtgctcc ggatgctcgt gatggggtac aaaatccccg atgacatcaa gatcagcctg
     2881 ccgcctggac cgcagaaggc caacatcaac gaggtgaaaa agcagacagt cggagggcac
     2941 ccgcccagtg tcctgtatca actcctgatg ggggacacga cgagcacacg agaccggatc
     3001 gccgccgctc acgtcatgac aagcctcttc acgatggtct tcgaacccgg tggaacaaac
     3061 gctccgcacc accacgaaac ggaggaagag atctatctgg tgcttgacgg ggaaggtgaa
     3121 atggtggccg gcgggggtat gaatggagtg gagggccgcc acccggtgaa acccggcgac
     3181 gccttcttct tccgcctcaa cgccacggtg ggcttctaca gttccaccgc gccgggcaac
     3241 aaggcccgca ttctggcggt gcgatccagg tacccgttcg gacggcgata gcgggctttc
     3301 ttccatcctg gcaccaacca atcccggcac gatatcgtca taattggaga cgtttttccg
     3361 aatgcgatcg attgcgcgat ggctgctgct gacggcgttg ctgacgctcg actttccggc
     3421 atgcgcatgg ggaccaacag gtcatcgggc cgttggccgg ttcactgaga agtacctcac
     3481 cgcgaaggca gcccgggcgg tgaaggacct gcttggaccg gacagcctcg ccgaggccgg
     3541 aacgtgggcg gacgagatca ggtcggatcc tgcgtggaag cgggcgtaca cgtggcactt
     3601 cgtcagcatc gaggatgacg agacttacga gacagcgaag aaagagccgg ccggcgatat
     3661 tctcgtgaaa ctccaccatt tcgaatccgt tctgcgcaac gccaaggcga cccgggacga
     3721 cagggtgatt gccctgaagt tctttgtcca cctcatcgct gacatccacc aaccgctcca
     3781 cgtcggacgg cgggacgatc gtggtgggaa taacatcgcc gtgaactggc acggtagacc
     3841 ggaaagtctg catcgggttt gggattcgct aatgatcgac ggggagcggc tcagtttcac
     3901 ggaacttgcc gagtttctcg atccgccatc cgctcagcag atttcgacat ggcagaaaac
     3961 cggctacctc gactgggcgc gcgagtcgtg cgagctccga ggcagggtct acgacatcgg
     4021 agatggccaa cttggctacg attactccta caagaatttc ccggtcgtcg aggaacgact
     4081 gaggcaggcc gccatacggt tggcggggaa actgaactcc atcttcaagt agccccggaa
     4141 cttccaggtc actcccgttc gatgcccagt ttcttgatct tctcctgaag gctttgacgc
     4201 tccaggccga tggcggctgc cgtccgcgtc acgtttccct gattctcccg aagcttccgg
     4261 ctcaggtagg cggcctcaaa ctgctgccgg gcctcgcgaa aatcgggtgg agcgaggagg
     4321 tcgtcttcca gaggcgatgc cggcggaccg gattgcgtgg ccgcagacac gtcgtcttcg
     4381 atgacccgtt ccccgcgagc gagaacaaaa agacgctcca ccgcgttgcg caattggcga
     4441 acattaccgc gccagggagc ttgctggagg ctgaccatcg cctcgcggga gagttccggt
     4501 ccagggcgtt cgtaccggtg tgaacactcg gcccaaaact gctcgacgag tagcgggata
     4561 tcctcgcggc gctgtcgcaa cggaggaagg tacagggtga cgccggcaag cctgtagtat
     4621 agatcctgcc ggaaacgtcc ctcctggatg gccttgtcga ggtcctggtt cgtcgcgctg
     4681 atgatgcgta cgtccacgcg gacctctttc gaatctccaa gccgttcgac gacacccgac
     4741 tcggccgcac ggaggatttt cgcttgggtc gccggggcca tatcgccgat ctcgtcgagg
     4801 aagagcgttc ccccctgggc gagctcgaaa cggccgggac gcgatgctgt agcgcccgtg
     4861 aacgctccct taacgtagcc gaacagttcg ctctccacca atgtttcggg caccgcggcg
     4921 cagttgagcg ccacgaaacg ctgccctgcg cggttgctgc gggcgtggat ctcctgggcc
     4981 agcaaatctt tgccagtgcc gctttccccc aggagcagga cgggcagttc cgtttgggcg
     5041 actcgctcgg ccagcacgaa gagctcgcgc atagcggtgg aggcgccgat catgcggccg
     5101 aactgccctt cggccgcaag acgctcccga agagcgtgaa cctcgttcct cagttgttgc
     5161 cgctcgagcg cccgcgccag cagcaggcgc agttcgtcaa ggtcgtatgg cttggcgaga
     5221 tagtcgtatg cacctgcttt catggcatcc acggcgaggc gctccgaacc gtgggcggtg
     5281 atcatgataa cggccggcga ctcgggttcc gcctgcaatt cgcgcagcag caccatgccg
     5341 tcctcctcag gcatgttgta gtccaggagg ataacgtcgg gccggtccga tagcaccttc
     5401 tcacgcgcct cggcgacgct cgatgcctcc acaacgcggt gttcggcatg gaaggcccgg
     5461 acaatcgcat agcgggcgac gggctcgtcg tcgacgacca ggacagtgcg aggcatcggt
     5521 attgtcgaat aactcatctt aaaccggccg acgcccgaga gttcgacagc aatggccatc
     5581 agcggaaagc cagcccgata cggaagccga cgagatgcgc gaggctgccg ccgtcagaga
     5641 agacttgatc ccggacctcc agccgtaggc cgacacgctc gctgaaccag gagatcacgc
     5701 cgccgccaac attgaaaccg ctctcggagc cggagcgaaa gagcagggta tatcctccgt
     5761 ttacgaacgg cacgaacttc gccgaagctg agcgagcgtt gcggaaaaag tagccgccgt
     5821 ccacggagaa gaccccgagg ccttgccgga agccacgcca tggatgcgca tacccgatct
     5881 cggcgccggc tgacaatccc ttgtacacaa gtccctcagc accggctccg taatgcacga
     5941 aaccggatcc gtcgggcgaa gtcacgcccg gagcaaagaa agcgtagcct tggccactga
     6001 agtccttctc agtttgcgct cccgcaggcc cagcaataac ggtcgccaag atcatcacca
     6061 aaaggatggt tttcacgatc tgttccctcc gaccttgacc tacgcaattc gcggactctt
     6121 tacgggccct tgtgtctcaa tgacttgcat acgtccacct gctcgcggtt tgactccatc
     6181 cgctgtcacc tgtcagaaag ggctggcagt cacgagcgct agaatggacg aatgggcgca
     6241 cgaaatcgct gggttctcgt tgtcacgtgc ctactcgtgg tgccgccctg ggcgcaaggg
     6301 gcaggtgtga cggtgcgatt tgaccccttg tcgccggaag tcgggccgtt tccggccgat
     6361 tccctgacgg ttgccgacgc aacacagaag accggggtgc gggtgaacct cccgctatcc
     6421 aactgcgcag cccagccaag cacatgcgaa gacctcaagc tgatcaacga gcttgacgga
     6481 ttcaacctcc agcctcgagt tcgggtgcga ttcagcgggc cggtcaacac ggcgacgctc
     6541 cggtccggga tatttgtggt cgctttggac gatattgtcg ctagtgtact tggggtgcat
     6601 ctggcggggc aagtcatcgc gatcaaccag gtgatcttcg atcccgatac gaatacggcg
     6661 tacggcgagg cggacgaagt gctcgaccag catcgccgtt atgctcttgt ggtcacggat
     6721 gcggttcggg acgcctcggg cgatcccgtc gaacaggacc cgacctatgg acggtgtttg
     6781 gcaccgctgt tccagagcgc ctattgccgc cagctctcgg aagtcgtgaa gacgttgaga
     6841 gtcccgggga agatcgtggc ggcatcgcgg tttacgactc tgagcgctac gagctggctg
     6901 gagaaggctc gggcaagaat tcaaggctcc acggtaaacg tccgtcggcc gtcaccgaaa
     6961 aacacattca aagtcgccag cctattggcg ttcagcttgc gccttcaggt gaaatccaac
     7021 cccccggcgt ttgaggattt cacagtgccc cagccgctat tagcggcgat cctggcggac
     7081 gtcgatcgga tcgcctttgc gtcgtttgac tcgccgcgat tcttggacgc caaacagaca
     7141 atcccggcag taccgaccgc tggggaagtc gcactgccat ctgccacgac tggagttcaa
     7201 ttccacgcct ttctgccgaa gggcgacaag ccgaaaacag gttatccggt ggtgattttc
     7261 gggcacgggc tcaacgacag ccgtttcggc gcgcccacgc tcgtcgccaa cacgttcgcc
     7321 cgaaacggct ttgcgaccat cgcgatgaac gccgttgggc atggctccgg accggagagc
     7381 acagtcagcc tcctggaact ctcgggaagc acgacgttga cggcgggcgg aagaagcatc
     7441 gacctgaatg gcgacgggag catcgagtcg cgggagggct gcctgctccc agggccgggg
     7501 cctttcgggt tgcgggactg tcttcggcaa acggctctcg acctcgcgca gtttgtgcgg
     7561 gttctccggg ccggcctcga tgtcgatgga gacggcagcg tggacctcga cccgggccgt
     7621 atctactatg ccgggcagtc gctcggcgcg ctttacggaa ccgtgttcaa cgcgattgaa
     7681 cccgaaatcc gggtgacggc gctcaacgtg ggcggagcga ctgtcatgga cgtagcccgc
     7741 tggagtccgt ctttccactc cgtgacgcgc gatttcctgg gtacccgtat gccgccgctg
     7801 ctaaatgcgg gcggggactt caacgagaac tacgttcttc gaggcgaacc ggcgaagacg
     7861 aatgacgtga ccggagcgat caatatccaa aacctcttcg agacgtccga atggcttcag
     7921 atgagcggcg acccgatcgc ctatgcggct catttgaaac tggcgccgct ggcgggcgct
     7981 actccgaagc gggtcttgtg gcagttcgcg cggggcgacc gcaccgtgcc gaatcctcag
     8041 aacagcgcgc tgattcgcgc cgccgggatg agagaagaga gccggctcta ccgacacgat
     8101 cttgcgcgcg cggtggcgcc ggatctttcg gagaacccgc acgcatttct cgtcgacatc
     8161 cagagcgccc cgggcttcgc gatcgcggcg gcagcgcaac agcagatcgc aggtttcttc
     8221 gctgccgacg ggatgcagat tcccgatgtc aacaaactcg taccgctgct gttcgggcgc
     8281 aacgtgttcg agacgcccgc ctcgctgccc gaggacttcg gcttcggcgc gtcggtcgtg
     8341 agcgtttcgg cggcgagtta cgatttcgtg gtcgcgccgg agtcgatcgt cgccgcgggg
     8401 ggtacaggtc tggcagtgcg gacggaagcg gcgacgtcgc ggtcgctgcc gacagcaatc
     8461 gccggcacca cggtacgggt ggtggattcc acgggggtcg agcggttagc gcccctgttc
     8521 tttgtttcac cacagcaggt gaactacatc ctaccggcgg gcacaactcc ggggatggca
     8581 agagtgaccg tcacaagcgg tgacggggag gcctcgtccg ggacggcgca ggtggaatct
     8641 gtggctccgg cgctgtttac ggcgaatgcc agcggctcag gcgtagcggc tgcagtagcg
     8701 accctggcaa aagtggacgg gtcgcaatcg agcgacgtcg tgttccagtg tctcgccggt
     8761 cccggaacgt gcgtggcgaa gccgatcgac ctaggagcgg caggagaccg ggtctacctg
     8821 ttgcttttcg gcacgggaat ccgtggccgg agcggcttaa cagctgtgcg cttgaccctt
     8881 gacggcgcca atgttccggt cgattacgcc ggggcacaga gcgagttcgc gggactcgat
     8941 caggtgaatg ccgggccgat tccgcggtcc ttcactggac gcggggaaac ggccgtcgaa
     9001 ctcacggtgg acggcaagcc cacgaacatc gtgacgatca gcttccgtta gctacgaacg
     9061 tgaagccggg cgcctagatg aacttgtact cgcccggcac aggcaacggc ggaacctcga
     9121 tgttcgcttc gtagctcatt gccttcggcg aaaggtcgcg cttggagttc atgatcatgt
     9181 cccatgtgac ctccagccca gagtaggcgg attcacgggc catgatgcag gtcatcgtgc
     9241 tttcggcgac cgccatggcg tggttgatgt acgggccgtc accgcgaatg ctgttgacca
     9301 tcgcggtgtg ctcgcggacg tacgggttct ccatcggatc gcggaacccg ccgcggacgt
     9361 cctggcccga accgcttccc atgtcatggg cgttgctgcg tcccttggcg ccgacgatta
     9421 actcgctcac gttcgtcgcg cagcccttgg ggtactgcct gcagtagctg gacatgtgga
     9481 cgccgtttgc gtactcgaaa tcggcgctga ggtggtcgta gatgttcccg taggcggctt
     9541 cccgcggccg ccagatcgcg ccgcccgagg cgacgacctt ctcgggatgc gtgcccatga
     9601 cccagttgca gacgtcgatg ttgtgcaggt gctgctcgac gacctggtcg ccgcagagcc
     9661 agacaaagga gtaccagttg cggttctgcc agatcatgtc gctccacttc gggtcgcgct
     9721 gtcccttggg gccgggccat cctccgccga gaaactggag cacggggccg ccgacccagt
     9781 acgcgtaggt agcgacaacg ttgccgatgg cgccgttctt gatcttgtcg atcgtttccg
     9841 tatagtcgcg ggcgaagcgt cgctgcgctc ccgacatcac ggtcagctta agttcctccg
     9901 attttttcgc ggcggccatg aagcggcgca cgcccacagg gtcagtgcca aacggcttct
     9961 cgcagaaaac gtgcttcttg gctgcgatgc aggcctcgaa gtgctccggg cggtaaccgg
    10021 gaggagtggc cagcatcacg atgtcgacgt cggaagcgat gaccttcttg tacgcgtcga
    10081 agccgacgaa gtgccgatcg ggcggcacct taacgcgatc ctgtagcttg ggatttttcg
    10141 tctcatcgcg tatcgatgcc agcgatttct cgagccggtc ttcgaaaatg tcccccatgg
    10201 cgacaacctc gacgttctcg gtacccgtca gcagattgac caccgcttcg gtgccgcggc
    10261 cgccacagcc cacaagtcct gccttgagtt tctccttgcc ggctccacgc acaagctctg
    10321 gccggattat ggtgaatgca gacgttcctg cgcctatcac gctggagttc ttaagaaaag
    10381 agcgccgcga cagattatcg gagtttctgt tcatccttct cacctcggac gcctattcta
    10441 tctcctaccg gtatgggcgt gcccggaccc gcagcgaact gcaattgggt tcccggctca
    10501 gggcgactga gcgacaatag aactttgttt tcctggcgtt ggcgaggagg cagacggcga
    10561 tgatccaggg agtgaacgtc gcggccgtga cgccccggcg cgtgaatgcg gacggcgtgg
    10621 accttggggc gacgctggag ctgatcgatt tcctatgcga caagggagcg ggcggcatag
    10681 cactgctcgg ttcgaccggc gagttttcgc atttcgacct ggagcagcgg ctgcatctga
    10741 tccagtttgg cgtgaagcga agccgggcgc cggtcctcgt caacgtgtcg cattcctatc
    10801 tggacgggac gctagcgctg gcgcgtggag ccgcctcgta cggagcagcc gccttgctgc
    10861 tgatgccgcc gatcttcttc cgctacccgc aggaggatat ccggacgttc tatcaggagt
    10921 tcgcgcgcga tctcggcgtc tctgtgccgg tatttcttta caacgtgccg ttttttacga
    10981 gcgagatcgc gtgtgaaacg gcggagggtc tgctagccga gggccacttt gccggtatca
    11041 aagactccag tggagaaatc gagtatgtcc aacgaatgat ctctctcaag cagcaacggc
    11101 cactgaccgt tctggtgggc aacgacaaga tctttacggc ggccagatcc gccggcgccg
    11161 atggcgtcgt ttccggtgtc ggttgtgcgc tgccggagct gatggtgggt ctcgacaggg
    11221 ccatcctttc cggcgacacg acgcagagag atcggttgga gctccgactt caagagttca
    11281 tcgattggat cgattctttc ccggtaccca tcggcgtccg ggaggcggtt gccctgcgcg
    11341 ggctgaagac tggcccgcat gcgactccgc tcggcgaggg aacgcaacgg cggctcggcg
    11401 aattcaggga gtggtttcag ggctggcttc ccgggatgct gaaggaggct tcgcgtgcgt
    11461 aaggggcttc tgattcttct gctggggacg gcgctctcag cccaggtcct tgtccccggt
    11521 ccacaggccg ccgtcttcct gtcgggggtt gacgattcag atcagcctta cggcctgtac
    11581 gtgcctaaga attacgacgc ttcgaagaag tatccgctgg tcatcagtct tcacggggcg
    11641 tggtcgaatc atcgtgtgaa cctccgccgt gtattcggca aaggcaaccg cccgggcgag
    11701 tccgacgcgg aggccacccg gtacttcccg ccgcttcgtg atgtcgattt catcgtggcc
    11761 tcgccgttcg cccgcggcac catgggtttc atgggaatcg ccgagcggga cgtgtatgac
    11821 gtgctggccg acgtgaagaa acgattctcg atcgacgagg atcggattta tctgacaggc
    11881 ctgtcaatgg gcggcggcgg cacgctttgg ctcgctctct cgcgaccgga tatctgggcg
    11941 gcgatcgctc cggtttgccc gtcgatcgca caaggcactc aagaactggc tgggaacgcg
    12001 tttcatctgc cagtgaagct gtttcacggc gacatcgaca ccgcggttcc cgtagacgtc
    12061 tcgcggacct ggcacaagct gttgctcggc ctcggcgtga aggctgaata cgtcgagtat
    12121 ccgggcgtca agcacaattc ctgggacaac gcttataaag acggtgcaat cttcgactgg
    12181 ttcgcacagt tccggaggaa tcgcttcccg gaccgcgtgc gattcgctac caggttttac
    12241 aagtacagtt ctgcgtactg ggtacggatc gacggactga ctactgggga actcgcgacc
    12301 atcgatgcga agtttacgga caaaaaccgg atcgagatcg acaccgcgca ggtggacggt
    12361 ttcacactgc aactcgccgg gcatccgatg ttcgagtctg gtagaccggt ttcggtgaag
    12421 cttaacggtg cggaactggc agcccagggc gcggaggccg tatcgttccg ctcctctcgg
    12481 ggcgtctggc aggccggcca gtacgcgcaa cccacaggcg gcaagcgaac cggcgtggaa
    12541 ggtccgatcc gggaagtcgt cagcggccgc catctctaca tctacggcac gatcgattcg
    12601 cccggaagcg gagaactcga acaacggcgc acggtttcgt ccttcgcctc cgactggcag
    12661 ggaccgagcg cccggctcgc gctatggctg cgctcagttg ccgacaaaga ggcgaccgac
    12721 gccgatcttt acggggcgaa cctggtgctg tttggaacga aggagacgaa cgcgctgatc
    12781 gcccgctact caaccttctt tccgctcgaa ctcaatgccg gcgcggccga ctacggactg
    12841 gtctttatag cgcccgtggg cgaccggctc gtgctcgtaa attcgggcct tccgtggtgg
    12901 accggggcgg accgtgcgaa acgtcccgga ctccggtacc tgtggtcgcc ctatcaagtc
    12961 ttgctcagtt tcggagattt cattttgttc aaggggtcgc tcgagaacgt gatcgccgaa
    13021 ggaaacttca cacgctcatg gaaggtgccg ccggaggccg ctgaaaagat gaaggccacc
    13081 ggagcggtga aagtcttacg atgaacaaca ccatcggtca attcggtcat gccggtcacg
    13141 agcttgcccg ctggatcgcg gattaccggc tatcgttcac gacgccagag cggagtgaag
    13201 ctaccatgat cgagtttgcg taatcgcatc gtggagaccg gagatccgtg ttccaagaac
    13261 ttctgcctct gctggccgct ctcgcgatcg cagccatagt ctacacagtg cgggaggtcc
    13321 accggcggcg attcacggtc cgtcctccac tgcccgatct gacattgcgc gaaggctggg
    13381 agttgtggcg gcgaggcggc gcgcagcatc ctgacataaa attctttccg gcagacgcag
    13441 cgctgaccga cggagtcgcc gcagagatgg agcgggcgct cccggccgcg gaggccggta
    13501 tttccggcgc ggatcacccc aggcaggctg tccgccgttc gatcctcgaa aacgccggag
    13561 tcgcgctcca ccttgaagcg atcatcgggc tgggacaaga ggagagaaaa gccctgctca
    13621 agggttaccg ggagggaatg gaagggctgc tccagaacgc gtggcgtttg tcggtccatc
    13681 ggtggatcct tctgcggttt tacggccggc tcaagttcga cgatgccgtt cccgacgact
    13741 ggttccagca ttacatgcgc gtggccaggc cgtacatccg ggagaaggtg agactggcgc
    13801 gggaacatgt actcgagcgc aacgaaggat cgggccagtt cgcgcaggtc tacgatcaac
    13861 tcctacacca gcttgccgaa gacacgctca agatccggcc aaaacgccgc ttcgtcgcgc
    13921 cggacttgtc gtaaaagcga tcctgtcacg cgcattttgg cgccttactt gcccccatcc
    13981 ttgccggttc ctagaaaacc gtctccgaaa aagaccggct tgtatccccg gttcgttacc
    14041 cagcgaatgg cgcgctccac gcgatcgcgg tagtcgggct catacgccac gtaatcggga
    14101 tagaaatatt gctcgtggat catcagctcg atgacttcag attgatgagg atcggcagcc
    14161 actttctcga ggtacggctc aatgtcgttc agcgccaccg tgttgaccac gatgtcatga
    14221 cggaagaaaa tgatgtcctc tgagttgtcc ttccagtagt cgcggccgct gagatacagt
    14281 acctcggggc gcgagaggta atacgagacc gacggccggc cgtcctgcat ccggaaatat
    14341 cccggcatta tccggatgcc ttcgtcccga agggcgcggg cgccttcgcg cgtcgtttct
    14401 ccccagtgaa ccgttgtgac cgggctcaac agttcgcggc ccgcgaaccg ctcgatctcg
    14461 cgcgtgacgg cgcggtaatc gtagcggatt tgttctccgg aagcacggat ataggggcga
    14521 tcaggcttgt tcgagcgcgc atgaaacgtg aggcgtatcc agtcccggtt ccgctgccat
    14581 tcgtcgcggt acttgtcggg catctgtgag aggttgaagt cctcggtctc gtagtagatg
    14641 ttgaagtgaa cctttgtgcc gtacttgcgg tgcatatcct gccagagagc gaggtagggg
    14701 ttctcgaaga tcgaacggta gcgctccgcg ttgcgggcga tgtcccggag gaaccagatg
    14761 ttgtcgtccg tggaaaaccg gtagcgcgga aacgagtcgc gatcccaaag gactgtgatt
    14821 gaatccttct caccgcccga agtctcggcg acgatctttg tttcccgatc gcggagcacg
    14881 atcggcgttt caaatgcgcc ttccttaacg ggaaccgaga tcccattgat tcgcagcggc
    14941 ccggcgctac cggcgagccc tttcacggtg atccgcaggc cctcgggcgt cgtctcgccg
    15001 tcgtgacgat tcaggatggc gccgtcaggc ggctcgaaga tgcgtggcga aatctggctg
    15061 cctgccgggg caacgaaagc gatgatgagg agcgccttgg tgtaccacct cagtgaattc
    15121 atgatccgtg acctcggatc aattatcacc ccagaccaag ggcgactccg tgcccccatt
    15181 atctcgccgt aagggcgttg cgcgttgcca cgagggtctt ccggagcgat tcctccggga
    15241 gttccttgac gatcggcatg atcaggccgc tgagcagcat cgggacatcg cgggagagtg
    15301 agatcgcctc acactcgaca tacgtgcctc cgtcccgctc ctcgaagcgc caatacgaat
    15361 ttagcctcca aagaaagccg tggtcatggc ctggcggcat ctccttttcg ctcgacgttc
    15421 ccgggtcttc aacctccgcg atacgggtgc tgtacgaacg gctccaggcg cgcctagcgc
    15481 tgagtcgccg gtagttgacg tcgtactccg tattaaggac cacggttacg atcttgtgct
    15541 ttcgcagcct cagatagacg cgatagcggt cgccatcatg gccaaggagc ttcgagtccg
    15601 ccacttccgg ggcatatacg ttcttatgat tgtcgtagtt ctgaagaaag gccagcgttt
    15661 gcgccagcgt cgcgccgggg atgaacgtag caccgatcca atcgtgaacc agcccatcgt
    15721 tgattcgcgt gacggccctc tctcgtgccg gctcgatcac cacgccagac cggcggagtg
    15781 cagtgagcct ctccgcacgt tcagcgcacc acaggaacga ggacccttgc gcgcggcgct
    15841 ccagggcggg ctctcttgct ttcacatagg cctggaactc cgcttcggtg tgcggctcga
    15901 tcctggttgt accgaccgcg gccctcgcgc cgggcagcca gccaagcagg gagagcgccg
    15961 cggacagcac tatgcgttcg ccgcggcttc ggcgtgcaac ccgttttcct gcaatcaaaa
    16021 gcatctactt tcagtatccg caattccgac catccacttg aactgccgtg cgccagcgcc
    16081 taacctatcg cattaggccg cgttcgcggt ttcaagcggc cggaattgga aaaccatgga
    16141 cttcagaata ttcagacatc tgccggcgtt gacgctggca gcctttctct ttgtgctcgg
    16201 accggccaga ccagcctgcg cccaggattc tcccgagaag attctgttga aggactaccg
    16261 gccgaaatcg atctacaaga tcccggtgac acagatcgcc aaggcgaagt attgggtcat
    16321 cgatatgcac tcgcacgact acgccaagac gccggagcag atcgccgagt gggtgaagaa
    16381 catggacgag gtcggcatcc agaaaaccgt cctgttgacg ggtgccgtcg gccccaggtt
    16441 tgacgagatt cacaagcaat actcaaagta tcccgaccgc ttcgagttgt ggtgcggctt
    16501 cgactacacc ggatacaacg aaccgggata cccggcttcc gccgtcaaag agctggagcg
    16561 ctgctaccgc gcgggcgccc gtggtgtcgg cgagatcatc gacaaaggca agggactcgg
    16621 atgggggaag aaccgtgcag acggtatgca tcccgacgat gcccgcatgg atccgctgtt
    16681 agagaaatgc gcggagctag gcatgccggt caacatccac gtagccgatc ccatctggat
    16741 gtacgagaag atggacaaca ccaacgacgg tctgatgaac gcatacgagt ggcggctcga
    16801 caatcagccc ggtatcgtcg gtcactccgg catgatcgac atcatcgaac gagccgtgaa
    16861 gaaacacgcg aagacaaagt tcatcgtgtg tcactttgcg aacctctgct acgacctgac
    16921 ccgccttggc gggatgttcg accgcaatcc gaatctttac gcggatatct ccgcccgcta
    16981 cgcggagaca gcgacgattc cgcgattcgt ctcgaagttc tacgcgaagt acgtcggccg
    17041 tctcgtctac ggcacggaca tgggctataa cacgagcatg taccgcatca cattccgcgt
    17101 tctcgaatcg cttgatgagc atttctacga atccgaacag ttcggttacc actggagcat
    17161 gaacggcttc ggattgccgg acaaggttct taagcaggtc taccgcgaca acgccctgaa
    17221 gatcctgaaa gcggggcggt gacgtcctat gaggccagtc cgttgtttga tcgcgcctct
    17281 ctgcgtggcg gctcaactgg ccatgggagc ggggccgata agcgtcgtcg agtatgacac
    17341 cagcacggag gacttcccca atccggagag gggtttctat gcgcaactgc tcctatcgga
    17401 ccgccgaccg ggttcatcct ccctctcggc ggctcagctt gagagcctgc gcctgaagaa
    17461 ccagacggtc atccgccggt tgtacgagat ttccagcttc cgcgacgggg atctttcacc
    17521 ggcgttcctc gacacgctcc gccacgattt cgacacggcg cgcaagtcgg gcttcaagct
    17581 gatcctccgc ttcgcttacg atttcaccgc gaagggtcag gatgcgccga agaatgtgat
    17641 ccttcgtcac ctcgatcaat tgaagccggt cttcgaggcc gacgccgacg tgatcgcatt
    17701 tcttgaggcc ggcttcgtcg gcgcgtgggg cgagtggcac agctcctcgc acggtctcga
    17761 cagggactcg gatgcaaagc gggaaatcgt tctgaagcaa ctggaggtac ttccgaagag
    17821 ccgcatgaca tctctccgta cggcccgcta cagagaggag gtcttcggct ccgaagccgt
    17881 tcgtccgctg acgctcgacg acgtccttca cgtcgtgccg agggcgcgcg tcgggtacta
    17941 caacgactgt ttcctcggca gtgacaccga tgtcggaacc tatctcggtg aggatgaggc
    18001 cgcgcgggct gtggagaagg acttcatcgc ccgggagaca cgattcgtcg tacagggcgg
    18061 ggaaacctgc gccgtcagcc ctccacgcag tgactgcgcc acagcgctcg ccgagcttgc
    18121 ccagatgcac tggagctatc tgaactcgag ctatcatccc ggcgtgctga agggctggcg
    18181 cgcgcaaggc tgcatggagg agatttcgcg taggctgggc taccggttcc ggctggtgcg
    18241 gtcttccatg acgggaacgg ctgcccaggg cggtgcgttc gagatgtcat tcgaaatcag
    18301 caacgacggg ttcgcgagcc cgcacaaccc acggctcgtc gagctcgtct tgcgcgaacc
    18361 ggatcgaggt ggggaataca tcctggcgct ggaggaggat ccgaggatgt gggcttcggg
    18421 agagacccgc aatgtcagcg tcagaggcgg aatcccgcgc gatgtgccgc cgggacgcta
    18481 tcaggtgttc ctcaaccttc cggaccctgc gccgtcgctc tacggaaagt cagagtactc
    18541 gatccgcctc gcgaacagtg ctctgtggca gccggaaacg ggttataact cgttgcgcca
    18601 gagagtcgaa atccaatccg ccgcgggtaa gcggtacgcg ggacggaact ggttcgtggc
    18661 gcggaagccg gcggtgaaat agcccggctc tctacgactc gtttacgtgc acgacttggg
    18721 ccggcgggaa gccgttgaac cacgtggccg aggcgttgct gtaggcgccg atgttctcgg
    18781 agtagaccag atcctcgatt tcaaggtccg gcagttcatc ggattgcgaa atgcagtcca
    18841 gcccgtcgca ggtctgcccg aagaccgcgc agatctccgt gtcgccctta cggaacgctt
    18901 tgatgtggta ctggcagtgg tcgaagatga tgcctgagaa cgtgtgatag acgctgtcgt
    18961 ctatgtagta gcaggtcttt ccgtcgcgga ccgccttgcc gatgatccgc gccactgaag
    19021 tagccgcgat cgccacgagg aaccttcccg gctcggcgag gatctgcatg tcggccggga
    19081 acagccgatt gatttcggcg ttgatctttc gcgccagttc gctgaacggc cggacatgcc
    19141 ggtcgtacgg tgcagggaag ccgccgccga tgtcgagaat cttgatttcg tgaccacgcg
    19201 tcttggcctc ccgcaagacc gccgctgaca tgttcagggc ctgcacgaag ttctcgaaat
    19261 tcgtgcactg gcttccgacg tgaaagctga tggcttccac gaccaatccg gcacgaaacg
    19321 cttcgtcgat caggtctacg gcttcgccag ggtcgcagcc aaacttggag gacaactcca
    19381 ccatcgagcc ggtgttcggc acgcgcagac gtagcgccaa acccgcctga ggcgcgtact
    19441 tcctgatctt ccgcagttcg gtcaggttgt cgtacgtcac caacggtttg taccgattga
    19501 gcgcttcgag agtttccttc ggtttgatgg gattggcgta gacgatcttg tcccagatga
    19561 aatcctgctg ctctttggca ggaaggcgac ggatgttctc gtgcaccagc atgaactcgg
    19621 gtagagaggc cacgtcgaag ctcgcgccca cgttgtacag cgttcgtacg atctcaggcg
    19681 cgggattcgc ctttaccgcg tagtaggcct gcactctggg caggttcctc cggaaacggg
    19741 cgtagttccg gcggatcatt tcgtggtcga tcaccaccac cggcgtgcca ttcttcctgg
    19801 cgatcgattg gagttgtctg gccgtcattt cctgggagcc gggtgtctca gtcccgcatc
    19861 aagccaaaac cggttcggac ccgtcgacca tcttcgcttt agtgtggcgg aagtattctt
    19921 tcgtcagcgc cttgatcatc atttcgcgct ggccgtaaag ccgccgcagc ttgcgcggtt
    19981 tgtgcttcgc cagcgcatag tgggtgagga tcggcatggc gatggtggtg tcggtgtagc
    20041 agaccaccgc gtcgggaagc cggtccgggt ccaccttgcc ccaactgacc gcctcgctcg
    20101 gcgtggcacc gctgagcccg ccggtgtccg ggcgagcgtc ggtcacctgg aggaagtaat
    20161 cctgtccgaa ctccttgatg cgcaacactt cctggatctg cggttcggtt tgaagcatga
    20221 aattcttagg gcttccgccg ccccacagga ccaccgcgct cttcccgccg gcgcgcttgg
    20281 cagcaagcac gaatgcggtg gtttcattaa cgtcgatcga tggattgatg cgcagcttgt
    20341 taccccgcaa ctccacgccc gccacgttca tgccgatcgt cgagtccccc ggggacgatg
    20401 tgtagcacgg caccccggca cgataagccg ccgccagcac cgacacgtct ttcaatccgg
    20461 ctttgcgttc ccactctgcg gcatacctgc cgagcaagta gtgcagctcg gcggtgccca
    20521 tctctttttg aaactcgggc tgaacgagaa tgtcacgcag gatctcgtcg gtggccatca
    20581 ggcagtcgct gtagccgagc aggacgtcgt agactcggac aatgtcgttg ttccgcagtt
    20641 ccgtgtcgtc catcttgaag ctgcccacgc gcaccgggta attcagcgca aaatgaaggt
    20701 cgtgatagag gttcgcgccg gtcgcgacga tccagtccac gaagccagcc ttgatcagcg
    20761 ggacgaccga ggagcagccg aggcccgcgg gcgtcagcgc gccggagagg ctcatgccta
    20821 ccgtgacgtc cggctccagc atcttttcga cgaaaagctg gcaactttcc ttgagccgcg
    20881 cggcgttgta ggccaggaat gtgtcgtcga cgatggccgc cagcttctcc ctgccagtga
    20941 ggttcctggg caggatgcgc ttgccggaga ggtatttgtc ccggttcggg cacgtcttct
    21001 tcttcagcca gtcgggcatt tccgacaggt cttcacgata acggcgatga gtcttctcgg
    21061 tcttggccat ggttgaaaat acgattatgg cagaaccccg caccgcgact tcgcagacgc
    21121 ccgccgcact tggctactca atgcctgccg aatgggaccc ccacgaggcg acctggctcg
    21181 gctggcccca taaccctacg gactggccgg acaagctcga cacggttcgc tgggtctacg
    21241 gcgagatcgt gcgcaaaatc tccgctggcg aacttgttcg cgtactggtc aactccaaga
    21301 cggaggagaa actcgcgcgg aggtacctca cccgtgcggg cgcggacaca ggccgcgtgg
    21361 aattcatcgt tcatccgacc aaccgcggct ggacccgcga cagcgggcca gtttttgtgc
    21421 gaaggcgcac cgggaagcgc gccgagacag cgatcgtcca cttctatttc aacgcctggg
    21481 ccaagtatcc cgactggcag aaggaccgcc gtgtcccgga gacggcagcg aagcggctcg
    21541 gcaagcgctt gttccacgcc gaatgtagcg ggcgcaggtt cgttctcgag ggcggcggca
    21601 tcgacgtgaa cggccggggt acactgctga ccaccgaaga gtgctatctc gacccggtga
    21661 cgcaagtgcg aaaccccgat ctcggccgaa gcgagatcga ggagaccctg aaggcatatc
    21721 tgggcgtcac gaatgtgttc tggctcggcg gtggcgtggt gggcgacgat acgcacgggc
    21781 acgtcgacga tatctgccgt ttcgtgaacg cgaacaccgt ggtgctgatt cgggaggaca
    21841 acccgaaaga catcaacttc cggccgctgg cggagaactg ggagcgaatc aaggacatga
    21901 ggctcgaaga cggcaccagg cccgaggtcg tggcgctgcc gatgcccggc ccgatttttt
    21961 tcgacggtga ccggctgccg gccagttacg ccaacttcta catcgcgaac gccgcggtga
    22021 tcgtgccgac gttcaacgat ccgaaggacc gtgtcgcgtt gggcatcctc ggcgagctgt
    22081 tccgggaccg tcccgtcgtc ggcatacatg ccgttgacct ggtgctcggc ttcggcaccc
    22141 tgcattgcct cacgcagcaa cagcctctcg ccgccgtatg atcggaagga agacatgacg
    22201 gacgacatca tcagccggat cgtgacggag aatcgccggg gacgcgcggc cggcatctgc
    22261 tcggtatgct cggcgcatcc gtgggtgctc gaagcgacgc tcgcgcagac tcttgcggcc
    22321 ggcggtccgg cgctcatcga gtcgacgtcc aatcaggtga atcagtttgg cggctacacg
    22381 gggatgacgc ccgcccgatt tgccggagac gtacggaggc tggcggagcg gatgggcttc
    22441 cccgaagggc ggttgctgct cggcggggac catctcgggc cgtatccctg gcgaaaacag
    22501 gccgcctcgg aggcaatggg gcatgcacgg gcgatggtgc gggattatgt gctggcaggc
    22561 tacgtcaaga tccatctcga tgccagcatg ccgtgcgcgg acgacgcgtc accggtgctc
    22621 gatcccgccg ttgcggcttg gcgtgccgcg gagctttgcc gcgcggccga ggatgcatgg
    22681 cgtgagttgc cggcaagcag cccggccccg gtatacgtga tcggcaccga agtgcccgtg
    22741 cctggcggcg agcaattgga gtccggcgct ccagccgtga cgaagcccga agacgccaaa
    22801 gcgaccgtgg agatgcaccg ggaagcgttc gccgcgctcg gtctggatgg cgcgtttgag
    22861 cgcgtgatcg gactggtggt gcagccagga gtggagttcg gctcggccat agtgttcggg
    22921 tatgaccgcg gccgcgcggc ggtgctcagc gcgcagttgc cggatcgtcc ggagttggtc
    22981 tacgaagctc actccacgga ttaccaaacg gcggaggctc tccgcgagat ggtggaagat
    23041 cacttcgcga ttctgaaagt gggcccatgg ctgaccttcg ccatgcgcga ggcaatcttc
    23101 gcgctctcga tggtggaaca ggagtggctg agcggcggcg tgcgtctttc gcgtgtgcgg
    23161 gaggcacttg aagaagcgat gctgaagaac cctgcgcact ggaaggacta ctaccagggc
    23221 tcggaagacg aactgcggct cgctcggaaa tacagctaca gcgaccgttg ccgctactac
    23281 tggcccgacc cggccgtaca agcggaggtg gacacgctgg tccgcaacct gacccaacac
    23341 ccgccgccgc taaccctgat cagccagttc ctgcccgcac agtacgacgc cgtgcggaac
    23401 ggaaccttgt cccatgattc ggccagcctc atcaaagacc gaatccgtga ggtgttaagg
    23461 ctctacgcgt cagcgtgcgg agagtgagaa gtgggggctg ccagagaggg gactttgcga
    23521 caaaaccctt aaacctgtgc agcagaccgg tcacccgagc gggctcacgg atttcgcaca
    23581 aaaacgacat ccacctccaa tactcgagtg ctagactggg acgcagcgga ctgctggcgg
    23641 atcaatgtcg ttcgctctct catctgcccc gccgcgccgc ttggtggctc tttttctggc
    23701 cctgacactg gcgccggcgg cgggcctggt ctggctgggg tggcggctgc tcgaacagga
    23761 ccgcgccctt gaggcacagc gcgctcagga acgtcgggag tatgccgccg acctggccgc
    23821 cagcgccctg agtcggaaac tctcccttgc ccggcagagg ttgggacaac cggagcgggc
    23881 ggccgatctg gcatctgcac acgattcgct ggtcgtcgtg ttccgccagc aacaagtcga
    23941 ggctttccca gcggggcgtc tactcttcta cccatttgtc cttccggaag ggatggctgc
    24001 tgagaacgcc ggccagcagt tccggacagg cgaagaatac gagtttcggc acaaagattt
    24061 tgcgaaggcc attgagaatt tccgaagctt ggttttctcg agagatctcg tcgcccgtgc
    24121 gggcgcgcat cttcgcctcg cccggaatta ccggaagtcg ggcgacctgg aactggcctt
    24181 agccgagtac ggggaactca ctcgcgccga acgagttgtg atcgacggtg ttcctgccga
    24241 actggtggcg cgcggttcgc gatgtgcctt gctcgcggaa ctcggccgat ccgatgagct
    24301 acttcgcgag gccagggtgc tttacgaaga tatgctgcgc gggcgttggc gtctcacccg
    24361 tcccgttttc cagttgtacg cttcacaagc ccgggggtgg cttcgtcgat ccgacgacgg
    24421 tgaacgcggg caactggctt tgtcagacac cgtggagcgg ctgtgggggc agtggcaggc
    24481 tgcgcgtgcc ggcgatcgcc cgatggcggg cgcaacggtc cttcgggt
//
LOCUS       DATIFQ010000257        33269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1614248, whole genome shotgun sequence.
ACCESSION   DATIFQ010000257 DATIFQ010000000
VERSION     DATIFQ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 33269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33269
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614248"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VLE22_12000"
     CDS             complement(<1..743)
                     /locus_tag="VLE22_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HSB15174.1"
                     /translation="MDKTNYRIVFGWYTWQVMRTILLLVLCLLAVCAFGGDHPAAFPN
                     IPDSYLEEAVEIPDFWVSTVDGVNRFLAKNVQSGSVQSIGTTAGGRTIRAVVYGTPRA
                     GKGTSTFSGALGFRDVRAFIGPDHGRKVYLGLASVHGGEFEGIAGMVNLISVLETGKD
                     LRGREWSEITAAAKSLDRIILVPVTNVDGRSRVPLRMEKFRGSDETVHEYFNTGGLAG
                     GKMIGWPQCKEFIPLDFSRVEFPGGYPNDA"
     gene            742..2787
                     /locus_tag="VLE22_12005"
     CDS             742..2787
                     /locus_tag="VLE22_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSB15175.1"
                     /translation="MVRHSVCALDCPDSCSILVTVEGKRAVKLRGNPKHPITQGFLCA
                     KVTRYLEREYSTDRILFPHRRTRAKGEARFTRISWDGALDEITTRLREISTKHGPEAI
                     LPYSYAGTMGLLNGSGMDRRFFHRLGASRLDRTICSSTGGAALTETIGFRYGAEPERF
                     RQAKLIIAWGANILGTNVHLWPFILDARRSGARFYVIDPVKNRTASLADRHFAPHPGS
                     DTALALGLMHVILNENLHDADYVERYADGFDALRRRVAEYPPERVEALTGIARQDIVA
                     LAREYATTRPAAIRLNYGVQRSERGGAAVRAISLLPALTGSWREPGGGLLLSTSQAFQ
                     FDRKSLERADLQWKSPLGREARLVNMTELGRALTALDDPPVKALVVYNSNPGAIAPNR
                     NLVIEGLRRPDLFTVVIEQMPTDTADYADILLPATTFLEHTDLYLAYGHYFLQLARPA
                     VPGPGEAKSNVEIFRLLAERMGFEGDCFRDSEDEMIRTLLASGHPFLDGITLKRLDRE
                     RFVRLNVSAEGEPFLPFAGGGFGTPSGKCEFGAETLDYVPPVESRLGSEMLRTRYPLE
                     LVSPKSDDSLNSTFGNRPDTDGRTALVSMHATDAAARGIAAGDQVRMFNSRGSCVLIA
                     AVNDDVKPGVACARSVRWGKMARDGQNVNALTSDRLTDLGSGPTFYSCLIEIEKCGD"
     gene            2775..3320
                     /locus_tag="VLE22_12010"
     CDS             2775..3320
                     /locus_tag="VLE22_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15176.1"
                     /translation="MRRLTGNTTISLLALALIAGAFGCKRSPKRVQVEATEETSTTLA
                     TMVHVADPRASVQLVKGFYDVEQNAWRWTMQKFSVTLRPPAGSAQKGALLRVQFAIPD
                     PVIEKLKSIRLAANVEGVPLEGQEYTKPGEQVYTAEVPAKALGGEAVVVDFALDKALM
                     PGASDQRELGVVVSTIGFEAK"
     gene            3317..5185
                     /locus_tag="VLE22_12015"
     CDS             3317..5185
                     /locus_tag="VLE22_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15177.1"
                     /translation="MKRAARAAWWVIPGFVCLAVYWPGLRAWFQQDDFAWLALKLRLA
                     ETGDIWTVLFEPMAQGTIRPLSERAFFLGLYSLFGLDALPFRIVAFATQLVNLTLAAS
                     ITNRLTGSRTAGFCAALFWGVSSAAALPMSWSSAYNQILCAFFLLTSFHFLLRYIETG
                     RRGYWLAQWVSFLLGFGALEINVVYPALAAAYTLLCARKYFRKTLWLFVPSALFVAAH
                     WWFAAKPESGLYEMHFGPSMAGTLWTYWRWALGPERLAMAGYELPAWAVSAATAVLTV
                     SLLGFAVWQLRRRRWLAAFLLVWFLAVIGPVLPLRDHVSVYYLTVAAAGLAMLGGWAF
                     ASAWRGRAWWKAVATLAAALYMASSIPAARTAAQWNYERSRAVRNLVRGLERAHELHP
                     GKIILLTRVKSDLFWAGINHKPYRLLGLRDVYLAPGSESGIEAHPELGEAADFVLPAA
                     PALLALEEGRAVVYAADGERLKNVTALYRAVARTGWVEATLPRRLDAGSPLFAAQLGP
                     EWYALEGGYRWMPKKATLRLGGPERAGQKLYVSGFCPSRQLAAGPLRMTVAVDSHRFP
                     PVVIDRGDSPFEFGFPLPAEAIGKRSMDVAVEVERTFVVPSDGRPLGLVFGNFAVR"
     gene            complement(5190..5423)
                     /locus_tag="VLE22_12020"
     CDS             complement(5190..5423)
                     /locus_tag="VLE22_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15178.1"
                     /translation="MRFDDLIRSGMTVREIRTRYPQTAPVLDNFGFRESCDDCSLEVV
                     SRKYGLSSRDVVDALNAAAFGRNYDSGRDRIVQ"
     gene            5532..6668
                     /locus_tag="VLE22_12025"
     CDS             5532..6668
                     /locus_tag="VLE22_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HSB15179.1"
                     /translation="MNIGVLRERVGSERRVALTPPVVRQLVEHGNIVWVEAGSGEGAL
                     IRDDKYIRAGAHLAYSPGEVLHRCEVLTKVSIPMPEELAMVPPGTTLMAFYHMAVASR
                     RLVERLCERSITAIGYEIIQTPDGRLPVLAAVSEIAGQMTLPLAMHLLRSSSGGRGIL
                     VGGSPGVPPAQVVILGAGVVGTWAARSASAAGARVTVLDVDTDKLRRLMEHLPQVTTG
                     LADPDTVAAMVVSADVLIGGVLLAGRKTPHVVTRQMVESMKPGSVIIDVAIDQGGCVE
                     TSRPMTLEEPTFVHHGVVHYCVANLTADMSRSCSMALSQALLPHLVRLSESGVEHALR
                     TSPELASGTYTYGGSCVRCSLADVIGVPCRPLAELLPAPSGEAG"
     gene            6686..8011
                     /locus_tag="VLE22_12030"
     CDS             6686..8011
                     /locus_tag="VLE22_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HSB15180.1"
                     /translation="MGWMELYRSKVKTADEALAVVSSGDRVYIHQGCQEPEDLVAAMV
                     RRASELRDVEVLHLATMGAADYSKPEYEGHFRHNSFFIGSNVRQAAQEWRADYIPVFL
                     HEIEGLFLNGALPIDVALIQCCPPDDYGYMSLGTGIDVTLAAAKHTRHLIVEVNDQSP
                     RTLGDAFLHVSKADTIVEASHPLAEYKMQKITDVHRAIAGHVANLIPDGATLQTGIGG
                     IPDAVLANLRNHRDLGVHAEMFSDGVIDLIQKGVINNERKTIHLHKVIAGFVLGTKVL
                     FDFIHDNPIFEFHPTAYTNDPFIIAQNDRMVAINSAIEVDLTGQVCSDSIGHLPYSGI
                     GGQVDFVRGAARSKGGLPIIALPATAKDGRVSRIAPVLKPGSGVVTSRGDVHYVVTEF
                     GVAYLHGKNLRQRAEALVQIAHPDFRDELVTAANSIGLLARKARVPAGE"
     gene            8016..10802
                     /locus_tag="VLE22_12035"
     CDS             8016..10802
                     /locus_tag="VLE22_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15181.1"
                     /translation="MLKRSSILVLTVAALWATAGAAQQQKPLPGSEECQGCHETGRRT
                     GKREAGMPPPFDAVALQASPHRELECSGCHADLAGKEMPHPEKLAHVECGTCHPDEQS
                     QYDVSLHGLAAKRGDTLAPSCKQCHGTHNILRKTDPRSPTSTMGVPRLCGGCHREGTP
                     VSATRNIPQANILENYADSIHGEGLFKRGLTVTAVCNSCHTSHFVLPHTDPRSSISKG
                     NIAKTCTKCHSQIESVHQKVIRGELWEKQPNLIPACVDCHEPHKIRRVFYTQGMADRD
                     CLRCHGNADLKVVRGGMTVSMYINEGEISRSRHARVACVQCHTGARPSHDRPCRTIEA
                     KVDCSICHASQVTQYHEGIHGQLVAKGSPDAPTCLDCHGTHGVQGRNEPHSPTYSRNV
                     PNLCAKCHRAGQKAAVRYRGKQVNVVEHYLESIHGKGLLESGLTVTANCADCHTAHRE
                     LPSRDPRSSVNRANIPETCGRCHRGIADLFNASIHSATRGDGKKQPVCSECHSAHSIQ
                     RTDATSFQLQMMDQCGRCHRAITESYFETYHGKVSRLGYVKTARCSDCHGAHDILPVA
                     DPRSRLSRANIVNTCGKCHTGSHRRFAGYLTHATHHDPEKYPFLFWTFWGMTTLLLGT
                     LTISGLHTLVWLPRSLQYRKELKATHDGHGEVYVRRFRRLHRNLHLIVIFSFFGLALT
                     GMMLKFSYAKWAQVLARLFGGFEAAGWIHRFCAVLTFTYFAIHIWDLLRKKRQSGKSW
                     RRFLLDPEGMLFSGNDWRELVGSVKWFLGKGPRPEYGRWTYWEKFDYFAVFWGVAVIG
                     STGLLLWFPTVFTRFMPGWMINVATTVHSDEALLAVGFIFTIHFFNTHFRPERFPMDT
                     VIFTEGVPLEEFRRDRPREYQQMVESGALEANLMPPPVPLAVKIWKRLGYAALTIGLL
                     LIALIIYAQLIAYR"
     gene            complement(10827..11699)
                     /locus_tag="VLE22_12040"
     CDS             complement(10827..11699)
                     /locus_tag="VLE22_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSB15182.1"
                     /translation="MPSLDRILLPVDFSGRCQGAVRYSEALAAQFGSELTLLHVLPPP
                     HYEFSALEVGGSVLNELFASRAAHVRKELNSYMERELQDVVVKRVLLEGDPARCIVGH
                     AHAERVGLIVMPTHGYGPFRRFILGSVTAKVLHDADCPVLTGVHLEEAPPIESIAFRN
                     VLAAIDLGDQSARTLEWANWMASSCGARLHLVHVTASIEGHAGEYHDPEWRDHLAGQV
                     RVEIAKLEQCAGTKAELIVESGDAPKVICKLAEQIKADMLVIGRGSAAGVFGRLRTNA
                     YAIIRESPCPVASV"
     gene            complement(11742..13202)
                     /locus_tag="VLE22_12045"
     CDS             complement(11742..13202)
                     /locus_tag="VLE22_12045"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HSB15183.1"
                     /translation="MTNPSTPRSPLRGWLSPVIHLSNNWLSLIGVVLVTTSAILWIFL
                     LPSTVSHKVDNPYFGIAAFLILPGVFLFGLAIIPVGIYIRFRGERRRGSYPTDFPPLN
                     LRNVDFRRLVQFIGVTSFVNVIIAGHATYTAVEYMDSVAFCGKTCHTVMAPEYAAYQN
                     SPHSRVECVACHIGPGASWFVKSKLSGAWQVFAVTFNTYERPIPTPVKNLRPARDTCE
                     ACHWPQKYGGDKLRIVEKFADDETNTMSKTVLLMRIGGGARGPGIHGTHLGPGIVIRY
                     GHTDEARQEIPWVEYTDSNGKSTVYRAEGVKPNSQQTAVREMDCMDCHNRPTHVFDLP
                     ERAVDRAMSDGRIPRDLPFIKKTGVELLKKNYATREEAAKAIPAALAAFFRERYPEVY
                     RQRRQDIVRSAGGLLDIYYRNVFPQMKVTWGVYPNNIGHTDFPGCFRCHDDAHTSAGG
                     RKVSQDCNSCHQLLAMEETAPEILTQLGLASNGGQQ"
     gene            complement(13199..14878)
                     /locus_tag="VLE22_12050"
     CDS             complement(13199..14878)
                     /locus_tag="VLE22_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HSB15184.1"
                     /translation="MIRNNLFRTKLCFLLWAAALSPALLGQEAVDCASCHDQVKKLEN
                     TPHAALTCAGCHSEITAYPHPKKTVKPACADCHDNIARDEARGVHGLARAKGNEAAPD
                     CAVCHGDIHEIQRPNSIAFRTAAPDTCGMCHSEIAERFKGSVHGKALAAKNTAAPICT
                     DCHGEHSIIAPGRSESPVSAGRIRETCGRCHGDLRLSRRFGLPADRVLSFDASFHGLA
                     GKAGSQSVANCASCHGYHDILPSSDPKSMVSAKNLPSTCGKCHPGAGRRFAIGPVHLL
                     AGGKEPAGVLWVREIYLVTIPIVIGLMALHNFGDWVRKVLQLRFRSRVPGLMFRSAST
                     ADNPVVAEFRMFPFERIQHALLLTSFSVLVWTGFALKYPDQWWARPLVMWESAFPLRA
                     TVHRVAAVVMMAAAVMHAVSLIVNRKLREHWYAMLPKVSDVRESLQNMAWLFGLSSTK
                     PALSSHGYIEKAEYWAVAWGTVVMAITGVALWAVNLSLRWLPKTWLDIATAIHFYEAV
                     LATLAIVVWHLYSVIFDPDVYPLDTAWLTGYSPRNRPHHGPHAPSGDSTKT"
     gene            complement(15363..15764)
                     /locus_tag="VLE22_12055"
     CDS             complement(15363..15764)
                     /locus_tag="VLE22_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15185.1"
                     /translation="METVQLSIANTNYAKALEETLKRNGTWEVRCVELPDPRIDGVLV
                     VDSEAFDRLPSSLPNPERVVLITRNDPAHLSLAWEAGIVSVVFENDPINTAVLAILAA
                     RLRLNELRKGSSHPDQPTPGRPPGPPKARGR"
     gene            complement(15800..16009)
                     /locus_tag="VLE22_12060"
     CDS             complement(15800..16009)
                     /locus_tag="VLE22_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15186.1"
                     /translation="MRFLKLALPLFALCAGLLVNTSRSFGKPEYTKKEKKGCTVCHVK
                     ANSKDLNDVGKCYEKKKSLQGCVTP"
     gene            16230..17570
                     /locus_tag="VLE22_12065"
     CDS             16230..17570
                     /locus_tag="VLE22_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSB15187.1"
                     /translation="MSKLVPEWQRANVLEAVFDQLSDALVLYDQSNVITGVNEAAEKL
                     FGMSAEEMVGRECGEVFRCGTCESGCGMLAGLSQAPTISNSTVRLHTDNGRERLAVIR
                     TRQILDDTGRVNGAVATIKDVTEEVAPQNREIIAESQGMREVLQFVRRVAASEATTIL
                     LEGENGTGKDLVAKTLHYQSLRQAEPFIAINCAAIPDTLLESELFGYEKGAFTDARAQ
                     KRGIFELADKGTLFLDEIGEIPLMLQAKLLRVLEEQCFRRLGGLKDIKLDLRVVAATN
                     KNLREAVREGAFRQDLYFRLNVIHITIPPLRERTEDIMPLVKFFVEHYNTKFKRHVEG
                     VTPDAARLLMNHDWPGNVRELRNAIERAMILEESSYITSASLPISISQSSARQSSSAP
                     AAAIPDAGLSLEDNERLLLVKALEKTKGNQTQAARLLKITRDTLRYKMKKFSLR"
     gene            complement(17600..19111)
                     /locus_tag="VLE22_12070"
     CDS             complement(17600..19111)
                     /locus_tag="VLE22_12070"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HSB15188.1"
                     /translation="MTHLVRIRLEPLGVTLEVKRGTPLQDVLFPYGVEFPCGGQADCR
                     GCRVRLVSGELFASLDEQVILSASEIERGWRLACRGVAEADITLDIAQWETVILSDDS
                     TFEFTPREGLGVAVDLGTTTLVAQLLDLATGRVLGVRTALNPQAAWGADVMSRIQFAM
                     TAGGAEKLAATIRGQIGELVCELLEGRASEPQLTEVTIVGNTVMHHLFCGIPVEPLSH
                     VPFEAVRDGLEVFSAGDLDWTCVAGTPVRFLPCFGGFVGSDILAGVLAVRMHESDEVV
                     GLVDLGTNGEIVIGNRERIVCASTAAGPAFEGGRISMGMRASTGAISEVHLSGGRLVC
                     HALGGVEPRGICGSGLVDAVAAGLDSGAVAPNGRLPGKGAWPLAPPVSITQADVRELQ
                     LAKAAIAAGIRIVCRRRGVEPAAVKRLYLAGAFGNYINRASARRIGLLDFPLDLVHPA
                     GNTALLGAKLALFQGDGREAYANLRARVEHISLAEDPEFMDIYVDEMGFPSRD"
     gene            complement(19108..20118)
                     /locus_tag="VLE22_12075"
     CDS             complement(19108..20118)
                     /locus_tag="VLE22_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB15189.1"
                     /translation="MNGRERILAMIDGRPVDCLPLMPITMMFAADRIGVHYGRYATDH
                     RVMVEAQLRTAEEFDFDYVSAISDPAREASDLGAAVEFFDNQPPAIIESQALLADKTA
                     LASLTFPDPLGGGRMHDRVKALALFQERAGGDKLIEGWVEGPCAMAADLRGINNLMTD
                     FVDDSAFVRDLFEFVVEMELRFAHAQVEAGAELIGVGDAAASLVGPAVYHEFVEPYEE
                     RLIEGLRALGARVRLHICGRTRRILDGMGRVGADIVDLDWPAPMAEARARMGPDQILL
                     GNIDPVSVLRGGTPATVREAIAKCHRDAGPRYVVSAGCEVVRDTPLENIRALTQYARS
                     SR"
     gene            complement(20115..20828)
                     /locus_tag="VLE22_12080"
     CDS             complement(20115..20828)
                     /locus_tag="VLE22_12080"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HSB15190.1"
                     /translation="MRLKLIACEVLYRELCAVVSRSVNQVDAEFLPKGLHDMGSDGMR
                     KRLQETIDRVDASKYEAVLLGYALCGNGIGGLVARTIPIIVPRAHDCITLFLGSRRRY
                     LDYFNSHPGVYFKTTGWIERGKNLHMLDRQMTFSYEELVAKYGEDDARYLYEELTKHY
                     RQFTYIEMGVEPDGSFEREVRGEAAQRGWTFEKVQGDLRLFRTLADGPWDDGDFLVVQ
                     PGQRIVVKLDNGIIAAEDA"
     gene            complement(20928..21800)
                     /locus_tag="VLE22_12085"
     CDS             complement(20928..21800)
                     /locus_tag="VLE22_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HSB15191.1"
                     /translation="MKPLIEQLIAGGPVVTDGAWGTQFQVLGLTPGELADTWNVSCPG
                     LVEDVARSYVDAGSRVILTNTFRSNRITLEAHGLAAQTAELNLAGVRISQTAADGGAF
                     VFASMGPSGKLLMSGEVTEKQLAGAFEEQAQALAAGRPDAIVIETMSDLAEAVIAVRA
                     AAATGLPVVACMVFDTGRNRDRTMMGNTPEQIARELVEAGADVIGSNCGQGIEGYVPV
                     CRRLKEATDRPIWIKANAGLPELAGGKTVYTTTPADFASHVPALIEAGASFIGGCCGT
                     TPEFIRAVCQAVRA"
     gene            complement(21797..22432)
                     /locus_tag="VLE22_12090"
     CDS             complement(21797..22432)
                     /locus_tag="VLE22_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HSB15192.1"
                     /translation="MADTSKLYDAILNGDSKTAVAVTKEALADNSDPLELVNKAMIPA
                     MDEVGRRFECEEYFVPELLLAARAMKGSLELIRPLLAERGAQPAGRVVIGTVKGDLHD
                     IGKNLVSSMLEGGGFEVIDLGADVAPEKFVQAVKEKNANIVCLSALLTVTMPSMKTTI
                     DALTSAGIREKAKVIIGGAPVTQQYADEIGADGYGENANVAVQIARRLVAK"
     gene            complement(22425..23699)
                     /locus_tag="VLE22_12095"
     CDS             complement(22425..23699)
                     /locus_tag="VLE22_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB15193.1"
                     /translation="MTSKERVLSALNHREPDRVPVDFGGTAVTGIHVSCVAALRDFFR
                     LEKRPVKVHEPYQMLGWIDDDLKEAMGLDVDGICSRKTMFGFPNENWREWRTDQGLDV
                     LVPEGFRITRDAKGDALIYPEGDTSVPPSGRMPKDGYFFDSIIRQEPIDEANLNVEDN
                     LEEFKPIGDADLAHFATEAKRVSVSGRAVIAGFGGTAFGDIALVPGPFLKHPKGIRDV
                     AEWYMSTTSRRDYVHRIFSYQCEVGLANLEKIRTVVGDAVDVIWVCGTDFGTQTSSFC
                     SAGTFRDLYFPYYKAVNDWVHKNTNWKTFKHSCGSVERFIPQFIESGFDILNPVQCSA
                     VGMGAEHLKSSFGDRLTFWGGGVDTQKVLPFGTPAQVWEQVLERCRVFAPGGGFVFNT
                     IHNIQARTPVENIIAMIDAVNEFNGRKVRLHG"
     gene            complement(23696..25345)
                     /gene="pckA"
                     /locus_tag="VLE22_12100"
     CDS             complement(23696..25345)
                     /gene="pckA"
                     /locus_tag="VLE22_12100"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HSB15194.1"
                     /translation="MNIYYDIESPAWKEAGQAASDFGLKNHGIINPRKVYWNLPTGAL
                     YEEAIFRGEGRVAHQGPLVVHTGKHTGRSAADKYIVREQKTEEHVWWGEYNRPYNPEN
                     FNALLARMQAFFQGRDVFVQDLFAGADPANSLPVRVITEKAFSSLFVRTMFLKPRTQE
                     ALKKHVPEFTIICAPSFLANPLVDMTRSETFIIANFSARLALIGGSMYGGEMKKTIFT
                     VLNFLLPLEGVMSMHCSANVGPDGDTALFFGLSGTGKTTLSADPKRRLIGDDEHGWND
                     NGIFNVEDGCYAKVIRLSASAEPQIYACTRRFGTIIENVVMDPLTRRLDLNDDRITEN
                     TRAVYPLEYIDNSVPEKMAGHPKNVIFLTCDASGVMPPIARLSPEQALYHFISGYTSK
                     LAGTEMGLGIEPEITFSTCFGAPFMVHHPHDYAELLRSKIVRHGVDCWLVNTGWTGGP
                     FGVGKRISIQHTRTLLDNVLEGKLRNAKFRQDPIFGFGVPEACEGVPADILDPSNTWG
                     DREEYFRKYDALAARFIENFKLFQDEVPRSLTEAGPKRWSR"
     gene            complement(25342..26559)
                     /locus_tag="VLE22_12105"
     CDS             complement(25342..26559)
                     /locus_tag="VLE22_12105"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSB15195.1"
                     /translation="MSLSQLARSIAESPTLKLNETAALLREKGEPVIHLGGGEPKSKT
                     PMDAILSCTSVLNTGDVKYTPADGIPALKKAIIRYTEEYYGRLVTPENVIVSSGAKQS
                     IMVLLHTILDPKDEVVFPSPYWVSYPEMVKLAGGVPVVAHPKDGTFQPTIEDIAEVVG
                     SYTKAIILNSPNNPSGVVYSKEFIAQVVEFCEKKSLYLIMDDTYNRLIFDGQTPTNCY
                     EYAKDLTESSKLIVVNCVSKMYAMTGFRIGWAVANKQVVRAMTNIQSQETSGPATPSQ
                     WAAVGALNGIQSSIQTLRMTLENNRNVMVARLNAIPGVKVTKPGGTFYCFPDFSAYNK
                     ESQKLAQFLLDKVRVVTVPGKEFGREGHLRLSYCGTLGEIKQGIERIQWAIDPNSPNE
                     LYIGGRKLVRDWA"
     gene            complement(26614..27810)
                     /locus_tag="VLE22_12110"
     CDS             complement(26614..27810)
                     /locus_tag="VLE22_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15196.1"
                     /translation="MPDCTQPRTERRRFIQTSAAAGGLLLLKPRTVFGTQANSALELG
                     IIGYGGRGSWICDLFTEFTTTRVVAAADPFQGRLEEARVKLKLDASRLYGGLDGFRDL
                     VASNLDAVAIESPPYFHPEQAEAAVSAGKHVYLAKPTAVDVWGCRSILASGGKTKGKL
                     SFLVDFQSRASSAFQEAVSRVHSGAIGSPVLGHIFYHTSRLNRKDTPGMTPAQARLSN
                     WVFDKTLSGDIIVEQNIHVLDVGNWYLRSHPLKACGTGGRRARVDVGDCWDHFVVTYW
                     YPNDVRVDFSSAQFIKGFHDMCMRVYGTAGTVEAHYGGAVKITGDNPWLGTDKDDTGR
                     LGTIANIRSFVESIRTRKYLNNASESVDSNLTAVLGRMAAYGDRVVTWDEMLRSNERL
                     DAKLKL"
     gene            27937..28013
                     /locus_tag="VLE22_12115"
     tRNA            27937..28013
                     /locus_tag="VLE22_12115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27971..27973,aa:Arg,seq:tct)
     gene            28191..28811
                     /locus_tag="VLE22_12120"
     CDS             28191..28811
                     /locus_tag="VLE22_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15197.1"
                     /translation="MERYWAPASSGLVQCAAQAVPETIYLKALGLLEFRSIDPETIGS
                     LRVRVFSKGAAVLAGLLALEASTSLNQEFEPPSYAEDPRVSTLSRFFEEKDSPVAYLA
                     RDFILVADWNGLDWRLLPAIAFVESGGGKAFRNNNIFGWDNGNWPFPSIRASIHFVGE
                     RLSASQLYEHKNVAEKLATYNPHSHYPLLVTSVMDEIGPSQFPPTN"
     gene            28984..30081
                     /locus_tag="VLE22_12125"
     CDS             28984..30081
                     /locus_tag="VLE22_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15198.1"
                     /translation="MQTALAPAGVAEGRAAPERFLPLDAFRGLIMVLLVSDGFGLRAL
                     AKHPVFHGLAEQFEHVPWEGAVFYDTIAPAFLFMVGMAVPYALGLRTERGATFRDNLR
                     HVAMRSLRLMLYSQVLISIQSHRLHFQMHNILTHIAVAYFLCFLIMQWPFRYQALAGV
                     GLLAAHTSLFFLFPGPDGPFTKTGNIGAVWDRALMGYNYASWTTNLNMISTTASALFG
                     VWAGNLLRSGRPRSEQMKILTVAMAAGFASGLALSPWVPIIRRIWTASLALYAVGWVL
                     LMFLTLYVPIEVGGWRKLAFPFVVAGSNAIFIYTLDMILRRWLDESVAVFTGKFTFLG
                     AFGPVAQSCAVLLVMWSLCYWLYRHRIFLRL"
     gene            30163..31956
                     /locus_tag="VLE22_12130"
     CDS             30163..31956
                     /locus_tag="VLE22_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15199.1"
                     /translation="MVILRLAGWRVAAAVFWIAVASPAAPRPDSDPVLHPGPEQVPEW
                     AQQGKYRFARLDGGPIEILKTARSSWGMHFTAREKEILGNLYSTYRDRMIDLLGQANL
                     NWVWITWSVGYSWEDEARQRDECRTLTSKLHEKGIRVAAYLCAASMFWESMFRDEPRS
                     TRWLRFDPQGVPFRYSSGRDPLRFVADVSNPEWVEYQKRRIGAAIDAGVDAFFLDNTS
                     QTGWSDNETMDRYIGRLRRYIREEKRSPAVLLTNYGLASNRIVLNRNMEVVFNEYWVE
                     PGVFGQEWNATNVRRMKYVRGVVPEWKPLISEYSRFHEGDRAHGWLKPKSAQLGIAEA
                     AAFGSAYAWDMEGPFDAALVDGDAGALETWRAIGRYNGFLKDNENLYWKARGAAPVAV
                     LISERRRRNVIQFGWDKDETGLYDLLASNSVLHDIRLVDALEGKQLAAYAGVVVPPFV
                     ESSARLTDVLKRFRTNGGKVVTLSETLIESLETNESARREVLEKVRGLAPARIGLAVE
                     GVPHVLGNITRLGSRRALAVHLLNYAPEAASGVRVRVNLGREFALLAGAQPRLLTPDL
                     QTEGLVTARSGASTVEFQIQNLDTYGVIVIE"
     gene            complement(31963..32469)
                     /locus_tag="VLE22_12135"
     CDS             complement(31963..32469)
                     /locus_tag="VLE22_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15200.1"
                     /translation="MTSRIYLVLAFLAFPLAGGELTHLGVPAGDHVLLEMVAGVRTGC
                     GPAKIDFVRTFPDGGAGAEPFRIPEDRVLVATDVDWYYFDGTPGIVQVLRLFVENLAD
                     PGKRRKVFESAAQLNGFGVGGANARMTTGFLVASDARLCVDVTPGPLGTPLRLSKVLV
                     RGYLVAER"
     gene            complement(32581..33015)
                     /locus_tag="VLE22_12140"
     CDS             complement(32581..33015)
                     /locus_tag="VLE22_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15201.1"
                     /translation="MPNNYSSKRILIAAGLLALLSVADVCAETKLVRIEIPFRFIAGD
                     QVLPAGVYRVAVDPAFNRMELRTEDGASGAFVSVYTAAKAPASDTGKLLFHGYGNTFF
                     LQGVWAAGMTSGYKLPASKAEREMAKVRSAGAQVAWLPIPAR"
BASE COUNT         6341 a        10202 c        10474 g         6252 t
ORIGIN      
        1 gcgtcgttgg gatagccgcc cggaaactcg acgcgcgaaa aatcgagagg gatgaactcc
       61 ttgcactgcg gccagccgat catcttgccg cccgccagtc cgccggtgtt gaagtattcg
      121 tggaccgtct catcgctgcc ccggaatttc tccatgcgga ggggtacgcg tgagcgcccg
      181 tctacgttcg tgacaggcac aaggatgatg cggtcgagcg actttgctgc ggcggtgatc
      241 tcggaccatt ccctgccccg caggtcctta ccggtctcga gcaccgagat caggttcacc
      301 atcccggcga ttccttcaaa ctctcctccg tgcaccgagg cgaggccgag atagaccttg
      361 cgcccgtggt ccggcccgat gaaggcgcgt acgtcgcgga agccgagtgc accggagaac
      421 gtcgatgtgc ccttgcccgc gcggggcgta ccgtagacaa cggcacgaat ggtgcgcccg
      481 ccagccgtgg tcccaatgga ctggacagag ccagactgga cgttcttagc caggaaccgg
      541 ttgacgccgt cgacggtgct cacccagaaa tcggggatct cgaccgcttc ctccaggtat
      601 gaatccggaa tattcgggaa ggcggcggga tggtcaccgc cgaacgcgca aacggccaac
      661 aggcacaaaa caagcagaag gatggtacgc atcacctgcc aagtgtacca accgaagaca
      721 atgcgataat tcgtcttatc catggttcgc cactcggtat gcgcgctcga ctgcccggat
      781 agctgctcca tactggtgac ggtagaaggg aagagggcgg tcaagctgcg cggcaacccg
      841 aagcatccga tcacgcaggg attcctctgc gcgaaggtga cgcggtatct cgagcgggag
      901 tattcgactg acaggatcct ttttccgcat aggcgcacga gggcgaaggg cgaagcaagg
      961 ttcacccgca tctcgtggga cggggcgctc gacgaaatca cgacgcggct ccgggagatc
     1021 tcgacgaagc acggccccga ggcgatcctg ccctacagct atgccgggac gatgggcctg
     1081 ctgaatggct cggggatgga tcgccggttc ttccacaggc tcggcgcgtc gcggctggac
     1141 cgaacaatct gctcgtcgac gggcggcgcg gctctgacgg agacgatcgg tttccggtac
     1201 ggcgctgagc ccgagcggtt ccgccaggca aagctgatca tcgcgtgggg tgcgaacatc
     1261 cttgggacga acgtccatct gtggcctttc attctggatg cacggcggag cggcgcccga
     1321 ttttatgtga tcgatccggt aaagaaccgg accgcgtcgc tggcggacag gcatttcgca
     1381 ccgcatcccg gcagcgatac ggcgctcgca ctcggcctga tgcacgtgat cctgaacgag
     1441 aacctgcacg atgcggacta cgtcgagcgc tacgcggacg gtttcgatgc actccgtcgc
     1501 cgcgtcgcgg aatatccgcc tgagcgggtg gaggcgctca ccggaatcgc gaggcaggac
     1561 attgtcgcac tggcccggga gtacgccacg acaagacctg cggcgatccg gctgaactac
     1621 ggcgtgcagc gcagcgagcg aggcggggcg gcggtgcggg cgatctcctt gcttccggcg
     1681 ctcacgggat cctggcgcga accgggaggt gggctgctcc tttccacgtc acaagcattt
     1741 caattcgacc ggaagagcct ggagcgggcg gacctgcaat ggaaatcgcc gctcggacgg
     1801 gaagcgcggc tggtcaacat gacggaactg ggaagggcac tcacggccct ggacgacccg
     1861 cccgtaaagg cgctggtggt gtacaactcg aatcccgggg cgatcgctcc gaaccgcaac
     1921 ctcgtgatcg aggggctgcg gcggccagac ctgttcacgg ttgtcatcga gcagatgccg
     1981 acggacacgg cggactatgc cgacattctg ctccccgcga cgacgtttct cgagcacacg
     2041 gatctatacc tcgcctacgg ccactatttc ctgcagttag cgcgcccggc ggtacctggg
     2101 ccgggcgaag cgaagtcgaa tgtcgagatc ttccggctgc tcgcggagcg catgggattt
     2161 gaaggcgact gcttccgcga cagcgaggac gaaatgattc gcacgctgct cgcaagcggg
     2221 catccgtttc tcgacgggat cacattgaag cgtctcgatc gtgagcgttt cgtacggctg
     2281 aacgtgtccg cggaaggtga gccgtttctg ccgtttgcgg gcggcggctt cggaacgcca
     2341 tctgggaagt gcgaattcgg cgctgagacg ctcgactacg ttccgccggt ggaatcccgg
     2401 ctgggttcgg agatgctgcg cacgcggtat ccgctggaac tggtctcgcc gaagagcgac
     2461 gacagcctca actcgacgtt cggcaaccgg ccggacacgg atgggcggac cgcattggtc
     2521 tcgatgcacg ccaccgacgc cgccgcacgc ggaatcgccg ccggtgacca ggtgcggatg
     2581 ttcaacagcc gcggcagttg cgtgctcatc gcggccgtga acgatgacgt gaagccgggc
     2641 gtggcttgcg cacggtccgt gcgttggggc aagatggcgc gggacgggca aaacgtgaac
     2701 gctctgactt ccgaccgcct gaccgacctc ggcagcggtc cgacattcta cagttgcctg
     2761 atcgaaatcg aaaaatgcgg agactaaccg gaaacacaac catttccctg cttgcgctcg
     2821 cgctcatagc tggcgcgttc ggatgcaagc gaagcccaaa gagagtccag gtggaggcga
     2881 ccgaggaaac gagcacgact ctggcgacga tggtgcacgt cgccgatccg cgcgcctcgg
     2941 tgcagctcgt caagggtttt tatgacgtgg aacagaacgc ctggcgctgg acgatgcaga
     3001 agttctcagt gacgctccgg cccccggctg gctcggcaca gaagggcgca ctgctgcggg
     3061 tccaattcgc gattcccgac ccagtcatcg agaagctgaa gtcgatacgc ctcgccgcga
     3121 acgtggaagg cgtgcctctg gaaggacagg agtacacgaa gcccggcgaa caggtgtata
     3181 cggcggaagt gccggcgaag gcgcttggcg gtgaagcggt cgtcgtcgat ttcgccctgg
     3241 ataaggcgct catgccgggc gcatcggacc agcgcgaact cggcgttgtg gtcagcacta
     3301 ttggcttcga agcgaaatga agcgcgccgc gcgcgccgcc tggtgggtca ttccgggctt
     3361 tgtatgcctc gccgtctact ggcctggcct gagggcctgg tttcagcaag acgacttcgc
     3421 ctggctcgcg ctgaagctgc gcctggctga aacgggtgac atctggacgg ttctgttcga
     3481 gccgatggcg cagggtacaa tccgtcctct gagtgagcgc gcgttctttc ttgggctgta
     3541 ctcgttgttc gggctcgacg cgctgccgtt tcggattgtg gcgttcgcga cgcaactcgt
     3601 caacctgacg ctggcggcgt cgattacgaa caggctgacc ggttctcgca cggcgggttt
     3661 ctgcgcggcg ctgttttggg gcgtcagcag cgcggcggcg ttgccgatgt cgtggagctc
     3721 ggcttacaac cagatcctgt gcgcgttctt cctgctcacg agctttcatt ttcttcttcg
     3781 ctacatcgaa acaggcagac gcggttactg gctcgcgcag tgggtgagtt tcctgttggg
     3841 tttcggggct ctggagatca atgtcgtcta tccggcgctg gcggcggcgt acacgctgtt
     3901 gtgcgcgcgg aagtactttc gaaagacgct ctggctgttc gtgccctccg cgctctttgt
     3961 ggcggcgcac tggtggttcg cagcgaagcc ggagagcggg ctgtacgaaa tgcatttcgg
     4021 accgtcgatg gccggaacgc tctggacgta ctggcgatgg gcgctcggac cggagcggtt
     4081 ggcgatggcg ggttatgagc ttccggcgtg ggcggtttcg gcggcgacgg cagtactgac
     4141 cgtaagcctg ctcggttttg cggtgtggca actccggcgg aggaggtggc tggctgcgtt
     4201 tctgcttgtc tggtttctcg cggtgatcgg accggtcctc cctctgcgcg accatgtgtc
     4261 ggtttactac ctgaccgttg ccgcagccgg cctggcaatg ctcggcggct gggcgtttgc
     4321 atcggcctgg aggggtcgcg catggtggaa ggcggttgct acgttggcgg cggcgctgta
     4381 catggcaagt tcgatcccgg cggcgcgaac ggcggcgcag tggaattacg agcgcagcag
     4441 agcggtgcgg aatctggtgc gagggctcga gcgggcgcac gagctgcacc ctggaaagat
     4501 cattctgctg acgcgggtga agagcgacct tttctgggct gggatcaacc acaagccgta
     4561 ccggctgctc gggcttcggg acgtgtatct cgcgccggga tcggagtcag gaatcgaggc
     4621 gcaccccgag ctcggcgagg ccgccgactt cgtgcttcca gcggccccgg ctcttctggc
     4681 gctggaggaa gggcgggcgg tcgtgtacgc ggcggacggc gagcgtctga aaaacgtaac
     4741 ggcgctttat cgcgcagtag cgcgcacggg ttgggtggag gcgacgctgc cgaggcggtt
     4801 ggatgccggc agtccgctct tcgcggcgca gctcggaccc gagtggtatg cccttgaagg
     4861 cggctaccga tggatgccga aaaaagcgac attgcggctc ggcggacccg aacgagccgg
     4921 tcagaagctg tacgtctcgg gcttctgccc gtcgcggcaa ctggcagcgg ggccgctgag
     4981 gatgacagtc gcggtggatt ctcaccgctt cccacctgta gtgatcgacc gaggcgactc
     5041 gccctttgaa ttcggcttcc cgctgccggc ggaggcgatc gggaagcggt ccatggatgt
     5101 ggccgtcgag gtggagcgaa ccttcgtggt cccgtcggac gggcgtccgc tggggctggt
     5161 gttcggtaat ttcgcggtgc ggtgaagccc tactgcacaa tgcggtcgcg gccggagtcg
     5221 tagttgcggc cgaaagcagc ggcgttgaga gcgtcgacga cgtcgcggga ggacagtccg
     5281 tacttgcgcg atacgacttc gagcgagcag tcgtcgcagg actcgcggaa cccgaagttg
     5341 tcgagtaccg gcgcggtctg cgggtagcgc gtacggattt cccggacggt cataccggaa
     5401 cggatcaggt cgtcgaagcg catagcaatc ccctcgttcc ccatagagta gcactcgcgg
     5461 atgcaggcgg gtgacgtgtg gcagcccacg tgcagtatga tttccttctt gcggggagtg
     5521 cgggagaaac gatgaacatc ggcgtccttc gtgagcgagt aggctccgag cggcgggtcg
     5581 ctctgacgcc gccggtcgtc cggcaattgg tggagcacgg gaacatcgtt tgggttgaag
     5641 ccggttccgg agagggagcg ctgattcggg acgacaagta cattcgggcg ggagcgcacc
     5701 tcgcctactc tccgggcgaa gtgcttcacc gctgcgaggt gctgaccaag gtttcgattc
     5761 cgatgcccga ggaactggcg atggtgccgc cggggacaac gctgatggcg ttctatcaca
     5821 tggcagtcgc gtcgcgtcgg ctggtcgagc ggctgtgcga acgctcgatc acggcgatcg
     5881 gctacgagat catccagacc cccgatggcc ggctgcctgt gctggctgct gtaagcgaaa
     5941 tcgccggtca gatgacgctt ccgctggcga tgcacttact taggtccagt tcgggcggcc
     6001 gggggattct ggtcggtgga agccccggag tgccaccggc gcaggttgtg atattgggcg
     6061 cgggtgtggt gggcacatgg gcagcacgat ccgcgtcggc ggcgggcgcc cgggtgacgg
     6121 tcctcgacgt agacacggac aagctgcgcc gcctgatgga acaccttccg caggtgacga
     6181 ccggcctggc ggacccggat actgtggcgg caatggtggt atccgccgac gtgctgattg
     6241 gcggggtgct gctcgcggga cggaaaacgc cgcacgtggt gacacggcag atggtcgaga
     6301 gtatgaagcc gggctcggtt atcatcgatg tcgcgatcga ccagggcggc tgcgtggaga
     6361 ccagccgccc aatgacgctc gaagagccga cgttcgtgca ccatggcgtg gtgcattatt
     6421 gcgtggcgaa cctcaccgcg gacatgagcc gcagttgcag catggcgctg tcgcaagccc
     6481 tgctgccgca cctcgtacgg ctcagtgagt cgggagtgga gcacgcgctg cgaacgtcac
     6541 ccgaactcgc cagcggtact tacacgtacg gcggctcctg cgtccgatgt tcactggctg
     6601 acgtgatcgg cgttccgtgc cggccgttgg ctgagctgct cccggcgcct tcgggcgaag
     6661 ccggataagg agatccggac aacatatggg ttggatggag ttgtaccgct ccaaagtcaa
     6721 gacggccgac gaagcgctgg cagtggtgag ttccggcgat cgcgtgtaca ttcatcaggg
     6781 ctgccaggag ccggaggatc tggtggcggc gatggtgcgg cgcgcctccg aattaaggga
     6841 cgttgaggtg cttcacctcg ccacgatggg cgccgccgac tattccaagc cggaatacga
     6901 aggccacttc cggcacaact cgtttttcat cgggagcaat gttcgccagg ccgcgcagga
     6961 atggagggct gattatatcc cggtcttcct gcacgagatc gaggggctgt ttctgaatgg
     7021 agcgctcccg atcgacgtgg ccctcatcca gtgttgccct ccggatgact acggctacat
     7081 gagcctcggc actggcatcg acgtgacgct ggctgcggcg aaacacacgc gtcacttgat
     7141 tgtggaggtg aacgatcagt cgccacggac tctgggagac gccttcctcc atgtctcaaa
     7201 ggctgacacg atcgtagagg cgtcccaccc gctggccgag tataaaatgc agaaaatcac
     7261 cgacgtccac cgggcgatcg ccggacacgt ggcgaacctg attcccgacg gcgcgaccct
     7321 gcaaaccggc atcggtggga tcccggacgc ggtgctggcg aatctacgca accatcgtga
     7381 cctcggcgta cacgcggaga tgttctccga cggggtgatc gatctgattc aaaaaggcgt
     7441 catcaacaac gagcggaaaa caatccacct gcacaaagtg atcgcggggt ttgtcctggg
     7501 caccaaggtt cttttcgact tcatccacga caatccgatc ttcgagtttc accccactgc
     7561 gtacaccaac gacccgttca tcatcgccca gaacgaccgg atggtggcga tcaactcggc
     7621 gatcgaggtg gacctgaccg gccaagtctg ctccgactcg atcggccatc tgccgtacag
     7681 tggcatcgga ggccaggtgg atttcgttcg cggcgcggcg cgctccaaag gcggcttgcc
     7741 gatcatcgcc ctgccggcga cggcgaagga cgggcgggtg tcgcggatcg cgccggtgct
     7801 gaagccgggt tccggagttg tcacctcgcg cggggacgtg cactacgtgg tgacggagtt
     7861 cggggtcgcc taccttcacg gtaagaatct acggcagcgc gccgaggcgc tggtgcagat
     7921 cgcgcatccg gacttccggg acgaactcgt gaccgcagcc aattcaatag gactcctggc
     7981 caggaaggcc cgggtgccgg ccggagaatg aagcaatgct aaaacgcagt tctatccttg
     8041 tgctgacggt agccgccttg tgggcgaccg ctggagcggc gcagcagcag aagccgctgc
     8101 ccggctccga ggagtgccag ggatgccacg agaccgggcg gcggacgggc aagcgtgaag
     8161 cgggaatgcc accgcccttc gacgccgtag cgctccaggc ctcgccgcac cgcgagcttg
     8221 aatgcagcgg atgtcacgcg gacctggcag gcaaggagat gccgcatccc gagaaactgg
     8281 cccacgtcga gtgtggaacg tgccacccgg acgagcagag ccagtacgac gtaagcctgc
     8341 atgggcttgc cgcgaagcgg ggtgacacgc tggccccgtc gtgcaaacag tgccacggga
     8401 ctcataacat tcttcggaag actgatccgc gttcgcccac ctccactatg ggcgtgccgc
     8461 gcctctgcgg aggatgccac cgcgaaggga cgccggtgtc ggcgacccgg aacatccccc
     8521 aagcgaacat cctcgagaac tatgcagaca gcatccacgg cgagggactc ttcaaacgcg
     8581 gtctgacggt cacggcggtc tgcaacagtt gccatacatc gcacttcgtg cttccgcaca
     8641 cggacccaag gtcttcgatc tcgaagggaa acatcgcgaa gacgtgcacg aagtgtcaca
     8701 gccagatcga gtccgttcac cagaaagtga tccggggcga actctgggag aagcagccta
     8761 acctgattcc ggcctgcgtg gactgccacg agccccacaa gatccggcgg gtcttctaca
     8821 cacaggggat ggctgaccgg gactgtctcc ggtgccacgg caacgccgac ctgaaagtag
     8881 tcaggggtgg catgaccgtg tcgatgtaca tcaacgaggg tgagatctcc cggtcgcggc
     8941 atgcgagggt cgcctgcgtg cagtgccaca ccggcgcgcg gccgtcacat gaccggccct
     9001 gccggacaat tgaggccaaa gtcgactgtt ccatctgcca cgcatcgcag gtaacccagt
     9061 atcacgaggg catccacggc caactggtcg cgaagggaag tccggacgca ccgacctgcc
     9121 tggactgtca cggcacacac ggggtacaag ggaggaacga gccgcactct ccgacttatt
     9181 cccgcaacgt gccgaacctt tgcgcaaagt gccaccgggc ggggcaaaaa gcggctgtcc
     9241 gctaccgggg caagcaagtc aacgtcgtgg aacactacct ggaaagtatc cacggcaagg
     9301 gtttgctcga gagcggtctg acggtaacgg cgaactgcgc cgactgccat acggcgcacc
     9361 gcgaactgcc gagccgcgat cctcgatcga gcgtgaaccg ggcgaatatc ccggagacat
     9421 gcggccggtg tcatcgcggc atcgccgacc tattcaacgc cagcattcac tcggccaccc
     9481 gaggggacgg gaagaagcag ccggtttgca gcgagtgtca ctccgcccac agcattcagc
     9541 ggacagacgc cacgtccttt cagctgcaaa tgatggacca gtgcggccgc tgtcacaggg
     9601 cgatcaccga aagctacttc gagacctacc acgggaaggt gtcaaggctt ggctacgtga
     9661 agaccgccag gtgctcggat tgccacggcg cgcacgacat ccttccggtg gccgatccac
     9721 gttcccgtct gagccgcgcg aacattgtca acacgtgcgg caagtgccac acgggctctc
     9781 accgcaggtt cgccggatac ctcacccacg ccacgcatca cgatccagaa aagtacccgt
     9841 tcctgttctg gaccttctgg gggatgacca cgctcctgct gggaacgctg accatctccg
     9901 gattacacac gctggtatgg ctgccgcggt ccctgcaata tcggaaggaa ctgaaagcga
     9961 ctcacgacgg gcatggcgag gtgtatgtgc gccgcttccg gcgcttgcat cgcaacctgc
    10021 acctgatcgt gattttcagt tttttcggac tcgcactgac cgggatgatg ctgaagtttt
    10081 cgtatgccaa gtgggcgcag gtcctcgcgc ggctgtttgg gggcttcgaa gcggcgggct
    10141 ggattcaccg gttctgcgcc gtcttgacgt ttacctattt cgccatccac atctgggacc
    10201 ttctgcggaa gaagagacag agcggaaagt cctggcgacg gttcctcctg gatccggagg
    10261 ggatgctgtt tagcgggaat gactggcggg aattggtcgg atcggtgaag tggttcctcg
    10321 gcaaaggccc gcggcccgag tacggccgct ggacgtattg ggagaagttt gactacttcg
    10381 ccgtgttctg gggcgtcgcc gtgatcggct ccaccggcct gctgctgtgg ttcccgacgg
    10441 tcttcacccg cttcatgccg gggtggatga tcaatgtggc gactaccgtc cacagcgacg
    10501 aggcgctgct ggctgttgga ttcatcttca ccattcactt cttcaacaca cacttccgtc
    10561 cggaaaggtt cccgatggat acggtgatct tcaccgaagg cgtgccgctc gaggagttca
    10621 gacgcgacag gccgcgggag taccagcaga tggtcgagag cggagcactc gaagcgaacc
    10681 tgatgccacc gcccgtaccg ctggctgtga agatctggaa gcggctgggt tacgcggcgc
    10741 tgacgatcgg cttgctcctg atcgcgctca tcatctacgc gcagttgatt gcataccggt
    10801 aggtacggcc tgggatcgtc cggccgctac acgctggcga ccggacaggg cgactcgcgg
    10861 atgattgcgt aggcgttggt acgtaggcgt ccaaacacgc cggctgccga accgcggccg
    10921 atgacgagca tgtccgcttt gatctgttcg gcaagcttgc aaatgacctt cggtgcgtcg
    10981 ccgctttcga cgatgagctc ggctttggtc ccggcacact gctcaagctt cgcgatttcc
    11041 accctgacct gcccggcaag atgatcccgc cattcggggt cgtgatactc gcctgcgtga
    11101 ccctcgatcg acgccgtcac gtggaccagg tgaagccgcg ctccgcacga agaagccatc
    11161 caattcgccc actcaagagt tcgcgcgctc tggtcgccga ggtcgatcgc agccagcacg
    11221 ttgcggaacg cgatcgactc gatggggggt gcttcctcga gatggacgcc tgtcaaaacg
    11281 gggcaatcgg cgtcgtgcag caccttggcc gtcaccgagc cgagaataaa ccgcctgaac
    11341 ggcccgtagc cgtgggtcgg catcacgata aggcctacgc gttcggcatg cgcgtgcccg
    11401 acaatgcagc gtgcgggatc gccttccagc aaaacgcgct taacgacgac atcctggagc
    11461 tcgcgctcca tgtaggagtt cagctccttg cgaacgtggg ccgcgcggct ggcgaacaat
    11521 tcgttcaaca ccgaaccccc aacttcgaga gcgctgaact cgtaatgggg gggcggaagg
    11581 acgtgcagca gcgtgagttc ggagccgaac tgggcggcca gagcttcgga atatcgcacc
    11641 gcgccctgac agcgtccgga gaaatcgacc ggcaagagga tcctgtccag tgagggcata
    11701 tggggcctcc ttccgcttct gaaactacca aaaaaaaacg gctattgctg cccgccgttg
    11761 gaagccaggc cgagttgcgt caggatctcg ggtgccgtct cttccatcgc caggagttgg
    11821 tggcaagagt tgcagtcctg ggagaccttc cggccgccgg cgctggtgtg cgcgtcgtcg
    11881 tggcaacgga agcacccggg aaagtcggta tggccgatgt tgttcggata tacgccccac
    11941 gtgaccttca tctgagggaa cacattgcgg tagtaaatat ccagcagacc ccccgcggag
    12001 cgaacgatgt cctgacgtct ctgcctgtag acctccggat aacgctcgcg aaagaaggcg
    12061 gcgagggcag cagggatcgc cttggccgct tcctcacggg tagcgtagtt cttcttcagg
    12121 agctcgacgc cggtcttttt gatgaacggc agatcgcgtg ggatccgccc gtcagacatg
    12181 gcgcggtcga ccgcgcgctc gggcagatcg aagacgtgcg tcggacggtt atggcagtcc
    12241 atacagtcca tctcccgcac cgcggtctgt tgcgagttcg gtttcacacc ctcagcgcgg
    12301 taaacggtcg atttcccatt gctgtcggtg tactccaccc agggaatctc ctggcgcgct
    12361 tcatcggtgt gcccgtaccg gatgacgatt cccggcccga ggtgcgtacc gtgaatgccg
    12421 gggccgcggg cgccaccgcc gatgcgcatc agcaggaccg tcttgctcat ggtgttggtt
    12481 tcgtcgtcgg cgaacttctc gacgatccgt agcttgtccc cgccgtactt ctgaggccag
    12541 tggcaggcct cacaggtgtc acgggcgggc ctcaggttct ttaccggagt cggaatcggc
    12601 cgctcgtagg tgttgaaggt gaccgcgaag acctgccacg cgccggagag cttgctcttc
    12661 acgaaccagg atgcgcccgg cccgatgtgg cacgccacgc actccacccg ggagtggggt
    12721 gaattctgat aggccgcgta ttcaggcgcc atcactgtat ggcaagtctt cccacagaaa
    12781 gccacgctat ccatgtactc gaccgccgtg taagtagcat ggccggcgat gatgacattg
    12841 acgaacgagg tgactccaat gaactgcaca agacgccgga agtcgacgtt ccgaaggttt
    12901 agcggcggga aatccgtcgg gtaggatccc cggcgccgct ccccgcggaa ccggatgtag
    12961 attccaacgg ggatgatcgc caagccgaac agaaacaccc cgggaagaat taggaacgcg
    13021 gcgatgccga aataggggtt gtcgaccttg tggctcaccg ttgaaggcag aaggaagatc
    13081 cacaatatgg ctgaagtcgt gactagaacg actccgatca ggctcagcca gttgttcgag
    13141 agatgaataa cgggagacag ccatccgcgt agaggggacc gtggggtgct tggattcgtc
    13201 atgttttggt ggagtcaccc gacggcgcgt gcggcccgtg atggggccgg tttcgagggc
    13261 tgtatccagt cagccaagcc gtatccagcg ggtagacatc cggatcgaag atcacagaat
    13321 acaggtgcca gacgacgatc gcaagcgtcg ccaggactgc ctcatagaag tgaatcgcgg
    13381 tggcaatgtc gagccaggtc ttcggcaacc agcgcaggct gagattcacg gcccacagcg
    13441 caacgcccgt gattgccatg accacggtcc cccacgcgac ggcccaatac tcggctttct
    13501 cgatataacc gtgcgacgag agggcgggct tcgtcgagct aagtccgaaa agccaagcca
    13561 tattctgcaa gctttccctc acgtcggaca cttttggcag catcgcgtac cagtgctcgc
    13621 gcagtttcct gttcacgatc agcgacaccg catgcatgac cgccgctgcc atcatgacga
    13681 cagcggcgac gcggtgcacc gtggcgcgca gcggaaacgc cgactcccac atcaccagag
    13741 gcctggccca ccactgatcg ggatacttga gggcgaagcc ggtccagacc agcacgctga
    13801 acgatgtcag cagcaacgcg tgttggatcc gctcgaacgg gaacatccga aattcggcga
    13861 ccacagggtt gtccgccgtc gaggcggagc ggaacatcaa gccagggaca cgcgaccgaa
    13921 atcgcagttg cagcaccttg cgtacccagt caccaaagtt gtggagtgcc atcagcccga
    13981 tcactatcgg gatcgtgaca aggtaaatct cacgcaccca aaggacaccg gcgggctcct
    14041 taccccctgc gagaaggtgc acgggaccga tcgcaaaccg ccggcccgcg ccaggatggc
    14101 acttgccaca ggttgacggc aggtttttgg cgctcaccat cgatttcggg tcggacgagg
    14161 gcagaatgtc gtgatacccg tgacaactgg cgcaattggc gacggattgc gagcctgcct
    14221 tgccggctag cccgtggaaa gaggcatcaa aactgagcac acggtccgcc ggcaacccga
    14281 accggcgtga caggcgcagg tcgccgtggc agcggccgca ggtctcacgg atccggccgg
    14341 cgctcaccgg cgattccgat cggccgggcg cgataatgga gtgctcaccg tggcagtcag
    14401 tacagatcgg agcggccgta ttctttgccg cgagcgcctt gccgtgaacg cttcccttga
    14461 agcgctccgc gatctcgctg tgacacattc cgcacgtgtc cggagccgcc gtgcggaacg
    14521 cgatcgagtt gggccgctgg atttcgtgaa tgtctccgtg acacacggcg cagtcgggtg
    14581 cggcctcgtt gcctttggcg cgcgcgagcc cgtggacgcc gcgggcctcg tcgcgagcga
    14641 tgttgtcgtg gcagtccgcg caggccggtt tgacggtctt cttcgggtgg gggtacgcag
    14701 tgatctccga gtgacaaccg gcgcaagtca gcgcggcgtg gggcgtgttt tcgagcttct
    14761 tgacctggtc atggcacgag gcgcaatcaa cggcctcctg cccgaggaga gccggagaaa
    14821 gagcggcggc ccaaagcagg aagcatagtt ttgtacggaa cagattattg cgaatcacta
    14881 cgtttcccgt cagggcctcc ttctcgagtt cctctagcca gtgtactgag agtttccgat
    14941 ccaggcctga taaatatagg ctgcggcgag agccagtcca gtgagaaccg caagcaacgt
    15001 caagatcttg ccccagcgct caatggactc cagtttcttc gccaattagc agtagccgcc
    15061 gcctcaactg cgctcacatg gatcgagtcg tcctctgcct tgccaacgag gctacggtaa
    15121 atggccaggg cgacgacgac ggcagccagt atgacccaac caatggcaaa aggaatcagg
    15181 ttcatcagtt ataagttcgt ttcagcaatc agcctcgcgc catcgttcgc agcgcccgca
    15241 cgaaccgtcg acgccgcgcg gcgcaaactt tcttttcaac cgcaaccttt agaaacacca
    15301 agacttacaa cgctcaccag tgcaaaactt cgttccagcc cgcagtcgaa caggggagac
    15361 gactacctgc cacgcgcctt tggcggcccc ggcgggcggc ccggagttgg ctgatcggga
    15421 tgagaggaac ccttcctcaa ctcgttcagg cgcagacgtg cggccaggat cgccagcacc
    15481 gccgtgttga tcggatcgtt ctcaaaaaca accgagacga tgccggcttc ccacgccagc
    15541 gacagatgcg caggatcgtt tcttgtaatc aaaacgaccc gctccggatt gggcagagac
    15601 gaaggcagcc gatcaaacgc ctcggaatcg acgacgagta cgccgtcgat tctgggatcc
    15661 ggcaactcca cacagcgcac ttcccacgta ccgttacgct tcagcgtctc ctcgagagcc
    15721 ttggcgtagt tcgtgttcgc gatggagagt tgaactgttt ccatggccgg cattgcgaac
    15781 cagcgacctg gtgatcagac tacggggtta cgcacccctg caacgacttc ttcttctcgt
    15841 agcactttcc gacgtcgttg agatctttcg aattcgcttt cacgtggcaa acggtgcagc
    15901 cctttttctc cttcttggtg tactccggct ttccaaaaga acgggacgtg ttgacaagta
    15961 gaccagcgca caacgcaaaa aggggaaggg ccaacttcaa gaaccgcatg tcgaataatc
    16021 ctccacatca gaacaaagca atcttgttgc caattcatac ttctcgcaaa caaattgata
    16081 caaatagagt tacgcgccaa tcgcgaattt tgtagttaac gccgggtgcg gaacctggcg
    16141 gtccggcgtc gagacctcaa tcaggtgggt gaattcgcgc actgcctgcg aatagctggt
    16201 atactgaaaa cagatttcgc tggctttcca tgtcgaaact ggttcccgag tggcagcgcg
    16261 caaacgtcct ggaggctgtg ttcgatcagc tttctgacgc tctggtgcta tacgatcagt
    16321 ccaacgtcat cacaggagtg aacgaggccg ccgagaagct cttcgggatg tcggcggagg
    16381 agatggtggg acgggagtgc ggggaagtgt tccgctgcgg cacctgcgaa tcgggatgcg
    16441 gcatgctggc gggactctcg caggctccca cgatcagcaa ctcaaccgtc cggcttcaca
    16501 ccgacaacgg gcgcgagcgg cttgcggtta tccggaccag gcaaatcctc gacgacacgg
    16561 gacgggtgaa cggcgcggtc gccacgataa aggatgtgac cgaagaggtt gccccccaga
    16621 accgggagat catcgccgaa tcccagggca tgagggaggt cctgcagttc gtacgccgcg
    16681 tagcggcgag cgaggccacc accattctgc tggagggcga gaacggaacc ggtaaggacc
    16741 ttgtcgccaa gacactccac taccaaagcc tgcgccaggc tgagccattt atcgcgatca
    16801 actgcgccgc catcccggac accttactcg agagtgagct gtttgggtac gaaaaaggcg
    16861 catttaccga cgcccgggcg caaaaacgcg gcatctttga gctggccgac aaagggacac
    16921 tgttcctcga cgaaatcggc gagattccgc tgatgttgca ggcgaagctc ttgcgtgtgc
    16981 tcgaggagca gtgcttccgg cggctgggtg ggctcaagga catcaagctt gaccttaggg
    17041 tggtggcggc aaccaacaag aatctgcggg aggctgtgcg cgagggcgct ttccggcagg
    17101 acctgtactt ccggctcaac gtgatccata tcaccatccc ccctctgcgc gagcgcaccg
    17161 aagacatcat gccgctggtg aagttcttcg tcgagcacta caacacgaag ttcaagcgcc
    17221 acgtcgaagg ggtgacaccg gacgcagcgc ggctgctaat gaaccacgac tggcctggca
    17281 atgttcgcga acttcgcaac gcgatcgaac gggcgatgat tctcgaggaa tcgtcctaca
    17341 tcacctccgc cagcctgccg atcagcatca gccagtcctc agcacggcag tcctcgtccg
    17401 ctcccgcggc ggcgattccg gatgctggtc tctcgctcga agacaacgag agattgctgc
    17461 tggttaaggc gctcgaaaag acaaagggta accagacaca ggcggcacgt ctactgaaga
    17521 tcacccggga tacactccgt tacaagatga agaagttcag ccttcgctaa ggcggccgcc
    17581 agccctcaaa cccggggact cagtcgcggc tcgggaaccc catctcatcg acgtagatgt
    17641 ccatgaactc cggatcctcg gcgagggaga tgtgctcgac tcgggcacgc aggttcgcgt
    17701 aggcctcgcg cccatcgccc tggaacaacg cgagcttggc gccgagcaag gcggtgttgc
    17761 ccgccggatg gacgagatcg agcgggaaat cgagcagtcc gatccgccgg gcgctggcgc
    17821 ggttgatgta gttgccgaag gcgccggcga ggtagaggcg cttgacggcg gcgggttcga
    17881 cacccctgcg ccgacagacg attcggatgc cggcggcgat cgccgccttc gcaagctgca
    17941 actcccgcac gtccgcctgc gtgatggaca ccggcggcgc gagcggccag gcgcccttcc
    18001 cgggtaggcg gccgttcggg gcaacggcgc cggagtcgag acccgcggcc acggcgtcca
    18061 cgagcccact cccgcaaatc ccgcgcggtt ccacgccgcc gagggcgtga catacgagcc
    18121 gcccgcccga cagatggacc tcagagatgg ctccggtgga ggcacgcatc cccatcgaaa
    18181 tacgcccccc ctcgaatgcc ggtccggcgg cggtcgacgc gcagacaatc cgctcgcgat
    18241 tcccgatgac gatctccccg ttagtcccaa gatccaccaa cccgaccacc tcgtccgact
    18301 cgtgcatccg cacggcgagc accccggcca ggatgtcgct gccgacgaat cccccgaagc
    18361 aggggaggaa ccggacgggc gtcccagcta cgcacgtcca gtctaaatcg ccggcgctga
    18421 aaacttccag cccgtcgcga acggcttcga atggcacgtg ggatagcggc tcgaccggaa
    18481 tgccacagaa caggtggtgc atcacagtgt tgccgacgat ggtcacctcc gtgagctggg
    18541 gctctgaagc acgcccctcc agcagttcgc agaccagttc cccgatctgc ccgcggatag
    18601 tggccgcgag cttttcggcg ccgccggcgg tcatcgcgaa ctgaatccgg ctcatcacgt
    18661 ccgcgcccca cgcggcctgc ggattgagcg ccgtccgtac gccgagcacg cggccggtcg
    18721 caagatccag cagttgcgcc accagcgtgg tagtacccag atcgaccgcg actccaaggc
    18781 cctcccgcgg agtgaactcg aacgtggaat cgtcactcag aatgactgtc tcccactgag
    18841 cgatatcgag cgtgatgtcg gcttcggcga cgccgcggca cgcaagccgc caaccccgct
    18901 cgatctcgga ggcggagaga atcacctgct cgtcgaggct tgcgaacaat tcgcccgaaa
    18961 cgagccggac gcggcagcct ctgcaatccg cctggccgcc gcaggggaac tcgacgccgt
    19021 acgggaacag aacatcctga agcggcgtac cacgcttcac ttcaagcgtg acgccgaggg
    19081 gctcgagtcg gatgcgtacg aggtgcgtca tcggctggat ctggcgtatt gggtaagggc
    19141 gcgaatgttc tcgagcgggg tgtcgcggac tacttcacag ccggcggaga cgacgtagcg
    19201 cggcccggcg tcacgatgac atttggcgat cgcctcgcga acggtagccg gagtgccgcc
    19261 ccggagaacg ctcaccggat cgatgttccc gagcaggatc tggtcgggac ccatccgggc
    19321 ccgggcctcg gccatgggtg caggccagtc aaggtccacg atgtccgcgc cgacacgccc
    19381 catgccgtcc agtatccggc gcgtccggcc acagatgtgc agtcttacgc gggcgccgag
    19441 cgcccggaga ccctcgatca acctctcttc gtaaggttca acaaactcgt ggtagaccgc
    19501 ggggccgacc agcgatgcag ccgcgtcacc gacgccgatc agttccgcgc ccgcttccac
    19561 ttgtgcgtgt gcgaagcgca gctccatctc gacgacgaac tcgaacaggt cgcgtacgaa
    19621 ggccgaatcg tccacgaagt cggtcatcag gttgttaata ccgcgtagat cggccgccat
    19681 cgcgcatggt ccctcgaccc agccctcgat gagcttgtcg ccgccggccc gctcctgaaa
    19741 cagggccagc gccttcacgc ggtcgtgcat gcgcccgccg ccgagcggat ccggaaacgt
    19801 caggctcgcc agggcggtct tgtcagccag aagggcctgg ctctcgatga tggcgggcgg
    19861 ctggttatcg aagaactcga ctgcggcgcc aaggtcggag gcctcccggg cgggatcgga
    19921 gatggccgac acgtaatcga agtcgaactc ctccgccgtg cgtagctgcg cctcgaccat
    19981 cacgcggtgg tcggtcgcgt aacgcccgta gtgcaccccg atccggtcgg cggcgaacat
    20041 catcgtgatc ggcatcaggg gcaggcagtc caccggacgg ccgtcgatca tcgcgagaat
    20101 ccgctctcgg ccgttcatgc gtcttccgcc gcgatgatac cgttgtccag cttgacgacg
    20161 atgcgctggc cgggctgaac cacgagaaaa tctccgtcgt cccatgggcc gtccgccagt
    20221 gtccggaaca gacgcagatc gccctgaact ttctcgaacg tccagcctct ttgcgccgcc
    20281 tccccgcgaa cctcgcgctc gaaactgccg tccggttcga cccccatctc aatgtaggtg
    20341 aactgccggt agtgcttggt cagctcctcg tagagatacc gggcgtcgtc ctctccgtac
    20401 ttggccacca gctcctcgta ggagaacgtc atctggcggt cgagcatatg gagattcttt
    20461 ccgcgctcga tccaaccggt cgtcttgaaa tacacgcccg ggtggctgtt gaaatagtcg
    20521 agataccgcc ggcggctgcc gaggaaaaga gtaatacaat cgtgcgctcg cggaacgatg
    20581 atcggaattg tccgcgccac aaggccgcca atgccatttc cgcagagggc ataccccagc
    20641 aggacggctt catactttga ggcgtcgacg cgatcgatcg tctcctggag gcgtttgcgc
    20701 attccgtcac tgcccatatc gtggaggcct ttgggcagaa actcagcgtc cacctggttc
    20761 accgagcggg acaccaccgc acagagctca cggtacagaa cttcgcaggc gatcagcttc
    20821 agacgcatag agacccagga aaaaggcatt gtaccgaagg cttgggggcc gatgtacggt
    20881 ggaccgtcct gtccaagctt cgcgggccgc ggtcctgctg gagcggttca ggcgcgaacc
    20941 gcctggcaga cggcacggat gaattcgggc gtggtaccgc agcagccgcc gatgaaggaa
    21001 gcgcccgcct cgatcagcgc cggtacgtgg gaggcgaagt cggccggcgt cgtcgtgtag
    21061 acggtcttcc cgccggcgag ttcgggaagt ccggcattgg ctttgatcca gatcgggcga
    21121 tccgtggcct ccttcagccg gcggcagacc ggcacatagc cctcgatacc ctgcccgcag
    21181 ttcgagccga tcacgtcggc gccggcttcg accagttccc gcgcgatttg ctcgggcgtg
    21241 ttgcccatca tggtccggtc cctgtttctg ccggtatcga aaaccataca ggcaactacc
    21301 ggaagcccgg tcgcagcggc agcacggacg gcgatcacgg cctccgcaag gtctgacatg
    21361 gtttcgatga cgatggcgtc cggccggcct gcggcgagag cttgcgcttg ttcttcgaag
    21421 gctccggcga gttgcttttc agtgacctca ccgctcatca gcagcttgcc tgacggtccc
    21481 attgaggcga agacgaaggc gccaccatcc gccgccgttt gcgagatccg gacgccagcc
    21541 aggttgagtt cggcggtctg ggcggcgagt ccatgggctt cgagcgtaat acggttggac
    21601 cggaaggtat tggttagaat gacacggctt cccgcgtcga cgtagctgcg cgcgacatct
    21661 tccacaagcc cgggacatga aacattccag gtgtcggcaa gctcgccggg cgtcaggccg
    21721 agcacctgaa actgcgtgcc ccaggcaccg tcagtgacca ccggtccgcc ggcgatcagt
    21781 tgttcgatca gaggcttcac ttcgcgacta ggcgacgggc aatctgcaca gcgacgtttg
    21841 cgttctcgcc gtatccgtcg gcgccgatct cgtctgcgta ctgctgggtc accggagcgc
    21901 cgccgatgat gaccttggcc ttctcacgaa tgccggcact ggtgagggcg tcgatcgtcg
    21961 tcttcatcga gggcatggtg accgtgagca acgcggagag gcagacgatg ttggcgttct
    22021 tttctttgac cgcttggacg aacttctcgg gagcaacgtc ggcgccgaga tcgatcacct
    22081 cgaaaccgcc cccctcgagc atcgacgaaa ccaagttctt gccgatatcg tgaaggtcgc
    22141 ccttgaccgt accgatgacc acccggcccg ctggctgcgc gccgcgttcc gccagcagcg
    22201 ggcgaatcaa ctcgagcgag cccttcatcg cccgtgccgc gagcagcaac tcaggaacaa
    22261 agtactcctc gcactcgaat cggcgcccca cttcgtccat cgccgggatc atcgccttgt
    22321 tgaccagctc cagcgggtcg gagttgtcgg ccagcgcttc cttcgtcaca gcaacggcgg
    22381 tcttggaatc tccattcaga atcgcgtcgt acaacttgct cgtatcagcc atgaagcctt
    22441 acctttcgtc cgttgaactc gttaaccgca tcaatcatag caattatgtt ctcaaccggc
    22501 gtccgggcct ggatgttgtg gattgtgttg aatacgaagc cgccgccggg cgcgaacaca
    22561 cggcatcgct cgagcacctg ctcccacact tgggccggcg tcccgaacgg caacaccttc
    22621 tgcgtatcca ctccgccacc ccagaacgta agccggtcac cgaatgatga cttgagatgt
    22681 tcggcgccca taccgaccgc cgaacattgc acaggattca ggatgtcgaa accggactcg
    22741 atgaattgcg ggataaaccg ttcgaccgaa ccgcaggaat gcttgaacgt cttccagttg
    22801 gtgttcttgt gaacccagtc gttcactgct ttgtagtacg gaaagtatag gtcgcggaac
    22861 gtgcccgccg agcagaacga cgacgtctgc gtgccgaagt cggtgccaca aacccagatc
    22921 acgtccacgg cgtcgccgac tactgtgcga atcttctcca ggttcgccag cccgacctcg
    22981 cactgatacg agaagatcct gtggacgtag tcccggcggc tggtggtgga catgtaccac
    23041 tcggcaacgt cgcggatacc cttcgggtgc ttgaggaacg gccccggcac aagcgcgatg
    23101 tcgccgaacg ccgtgccacc gaaaccagcg atcacggcgc gtccggaaac ggatacccgc
    23161 ttcgcctctg tggcgaaatg agcgaggtct gcgtcgccga tcggcttgaa ctcctcgagg
    23221 ttgtcttcga cgttgagatt ggcctcgtca atcggctcct ggcggataat gctgtcgaag
    23281 aaatagccgt ccttcggcat ccggccgctg ggtgggaccg acgtgtcgcc ttcggggtaa
    23341 atcagcgcat cgcccttggc gtctctggtg atgcggaagc cttccggcac gagcacatcg
    23401 agtccctgat cggtgcgcca ctcgcgccaa ttctcattgg gaaacccgaa catcgtcttg
    23461 cgcgagcaga tgccgtcgac gtcgaggccc atagcctcct tcaggtcgtc gtcgatccac
    23521 ccgagcatct gatacggctc gtggactttg acgggccgtt tctcgaggcg gaagaaatcg
    23581 cgcaacgcgg ccacgcaact gacgtggatt ccggtgaccg cggtcccgcc gaagtccacc
    23641 gggacccggt ctggttcccg gtggttcagg gcggaaagga ctctctcttt ggatgtcatc
    23701 tcgaccatcg cttcggtccg gcctcagtca gcgatcgggg aacttcgtcc tggaacagct
    23761 tgaagttctc aatgaagcgg gccgccaacg cgtcgtactt tcggaagtac tcctcgcggt
    23821 cgccccaggt gttggaggga tcgaggatgt ccgccgggac tccctcgcag gcctcgggaa
    23881 cgccgaaccc gaaaatgggg tcttgacgga acttcgcgtt gcggagtttc ccttccagca
    23941 cattgtcaag caaagttcgc gtgtgctgaa tgctgatgcg cttgcccacc ccgaaggggc
    24001 cgccggtcca accggtgttc accagccagc agtccacgcc atgcctcacg atcttggatc
    24061 ggagcagttc agcgtagtcg tgcggatggt gaaccatgaa gggcgccccg aagcacgtgc
    24121 tgaacgtgat ctcgggttca atgccgagtc ccatctccgt cccggcgagc ttgctcgtat
    24181 aaccggagat gaaatgatac agagcctgct cagggctcag gcgcgcgatc ggcggcatca
    24241 cgccagaggc gtcgcacgtc aggaaaatga cgttcttcgg gtgcccggcc atcttctcag
    24301 gtacggagtt gtcgatgtat tccagagggt agaccgcgcg cgtgttttcg gtgatacggt
    24361 cgtcgttcag atccagccgg cgcgtcagcg gatccatgac cacgttctcg atgatcgttc
    24421 cgaaccggcg cgtgcaggcg taaatctgtg gctcggccga ggccgacaga cggatcacct
    24481 tggcgtagca accgtcctcg acgttgaaga tcccattgtc gttccagccg tgctcatcgt
    24541 cgccaatcag ccgccgctta gggtccgccg agagcgtggt cttgcccgtt ccggagaggc
    24601 cgaagaacag cgccgtgtcg ccgtccgggc caacattggc cgagcagtgc atcgacatga
    24661 cgccctcgag cggcaggagg aagttcagca ctgtgaagat ggtcttcttc atctcgccgc
    24721 cgtacatcga accgccgatc aacgccagcc gggcggagaa gttcgcgatg ataaacgtct
    24781 cgctgcgcgt catgtccacc agtggattgg cgaggaacga cggcgcgcag atgatggtga
    24841 actccggcac gtgtttcttc aacgcttcct gcgtccgtgg cttcaggaac atcgtgcgga
    24901 cgaaaaggct gctgaacgct ttctcggtaa tcacacggac gggaagcgag ttcgccggat
    24961 cggcccccgc gaacaaatcc tgcacaaaaa cgtcacggcc ttgaaagaac gcctgcatgc
    25021 gggccagcag cgcattgaag ttttcggggt tgtacggccg gttatactca ccccaccaga
    25081 cgtgctcttc ggttttctgc tcgcggacga tgtatttgtc ggcggctgag cggcctgtgt
    25141 gcttgccggt gtgaaccacc agcggtccct ggtgcgccac cctcccctcg ccgcggaaga
    25201 tggcctcttc gtaaagggcg ccggtcggca gattccaata gaccttgcgc ggattgatga
    25261 tgccgtgatt cttgagaccg aagtcgctgg ccgcctggcc cgcttccttc caggccgggg
    25321 attcgatgtc gtagtagatg ttcatgccca atccctcacc agtttccggc caccgatgta
    25381 aagctcgttg ggggagttcg ggtcaatcgc ccactggatg cgttcgatgc cctgcttgat
    25441 ctcgcccagc gtgccgcagt agctaagccg gaggtggccc tcgcggccga attccttgcc
    25501 gggcaccgtc acaacccgca ccttatcgag caggaactgc gccagcttct gcgactcctt
    25561 gttgtatgca ctgaaatcag ggaagcagta gaacgtgccg cccggcttgg tgaccttcac
    25621 gccggggatg gcgttcagcc gcgcgaccat gacgttgcgg ttgttctcca aagtcatgcg
    25681 cagggtctgg atggaggact ggattccgtt caacgcacct actgccgccc actgcgacgg
    25741 cgtagccggc ccggaggttt cctgcgactg aatgttggtc atcgcgcgga ccacctgctt
    25801 attggccacg gcccaaccga tgcggaaccc tgtcatcgcg tacatcttcg acacgcagtt
    25861 gacgacgatg agtttggagc tttccgtgag gtccttggcg tactcgtagc agttggttgg
    25921 agtctggccg tcgaagatca gccggttgta ggtgtcgtcc atgatcaggt agagactctt
    25981 cttctcgcag aactcgacaa cctgggcgat gaactccttg gagtagacca caccgctcgg
    26041 attgttcggg ctgttgagga tgatggcctt ggtataggag ccgacgacct ccgcaatgtc
    26101 ctcgatggtg ggctggaagg tgccgtcttt gggatgcgcc accaccggaa cgccgccggc
    26161 gagcttgacc atttccgggt agctcaccca gtaaggggat gggaagacga cttcgtcctt
    26221 tggatcgagg atcgtgtgca gcagcaccat gattgactgc ttcgcaccgc tagacacgat
    26281 cacgttttcc ggcgtgacca gccggccgta gtactcttcc gtgtaccgga tgatcgcctt
    26341 ctttaaggct ggaataccgt cggccggcgt atacttgacg tcgcctgtgt tcagtaccga
    26401 ggtgcagctc aggatggcgt ccatcggagt tttgctctta ggctcgccgc ccccgagatg
    26461 aatgacgggc tcgccttttt cccgtagaag cgctgccgtt tcgttcagct tcagggtcgg
    26521 cgattccgcg atggaccttg ctagttgact tagactcata gctcgttaat ccttccgata
    26581 ttacattccg gaccgcccgg cggggaggtg cgtttacagc tttaacttcg cgtccagcct
    26641 ctcgttgctt cggagcattt catcccaagt gacgacgcga tcaccgtagg cggccatcct
    26701 gcccagcacc gcggtcaggt tactgtcgac gctctccgat gcattgttca ggtacttgcg
    26761 cgtccgtatg ctctcgacaa aactcctgat gttcgcgatc gtgccaagcc ggcccgtgtc
    26821 gtccttgtcc gtgccgagcc aggggttgtc gccagtgatc ttcaccgcgc ccccgtaatg
    26881 ggcttcgacc gtgcctgccg ttccgtagac gcgcatgcac atatcgtgga agcccttgat
    26941 gaactgggcg gaactaaagt ccacgcgcac atcattggga taccagtagg tgaccacgaa
    27001 gtggtcccag caatcgccga cgtcgacgcg cgcacggcgc ccgccagttc cgcacgcctt
    27061 caacgggtgc gagcgcaggt accagttgcc gacgtccagc acatggatgt tctgctcgac
    27121 gatgatgtcg cccgacagcg ttttatcgaa tacccagttg ctcagccgtg cctgcgccgg
    27181 cgtcatgccc ggcgtgtcct tacggttcag cctgctggtg tggtagaaaa tgtggccgag
    27241 cacaggtgac ccgattgcgc cggagtgcac ccgcgacacc gcttcctgga acgccgatga
    27301 cgcgcgggat tggaagtcca ccaggaagct cagttttcct ttggtctttc cgccgctggc
    27361 cagaatgctg cggcagcccc acacatcgac cgccgtcggc ttggccagat acacatgttt
    27421 ccctgcggag acggccgcct ccgcttgttc cgggtgaaaa taaggcgggc tttcgatcgc
    27481 caccgcgtcc aaattcgatg cgaccaggtc ccggaaccca tcaagcccgc cgtaaaggcg
    27541 cgatgcatcc aacttcaact ttacccgggc ctcctcgagg cgcccctgga aaggatccgc
    27601 cgcggcgacc actcgggtcg tggtgaactc cgtgaacaga tcgcagatcc aggagccgcg
    27661 gcctccgtag ccgataatgc cgagttcgag ggccgagttg gcctgcgtgc caaagacggt
    27721 ccgcggtttc agcagcagca aaccgcctgc tgctgcggag gtctggatga accttcggcg
    27781 ttccgtccgc ggctgggtac agtccggcat cgcgtctcct aaacgggatt tgtactattg
    27841 tagcgattct tttcagactc gcaagtccgg tgattttccg actggtttca accctgccgg
    27901 ccgccgtagt ctacaatcga agaggacaca ctccgcgggc gcgtagctca ggtggataga
    27961 gcatcggcct tctaagccga gggtcgcagg ttcgagtcct gccgcgccta ccacactgct
    28021 caaacggata cggtgggagg tggctggcgg gggcgagagg ccttttggct tggttcatga
    28081 cgccgggttg cacgtccatc gctcgccagt gagggtgatg accgcccagg cttcacccac
    28141 cccaaaagca ttcctcgaaa tcgcttttca ccgttggaac cttcaccaca atggaacggt
    28201 actgggcacc cgcatcttcc gggctagtac aatgtgctgc tcaagcagta ccggaaacta
    28261 tttacttaaa ggcacttggg ctcttagagt ttaggagtat cgacccggaa acgataggga
    28321 gtctccgagt gcgagtgttt tccaaaggtg cagcggttct cgccgggctt cttgcccttg
    28381 aggcgagtac cagcctgaac caggaattcg aacctccgtc ttatgctgaa gatcccaggg
    28441 tgtcgacttt aagccgattc ttcgaggaga aggactcgcc tgtcgcctat ctggcgcgtg
    28501 actttattct ggttgcggat tggaacggcc tggactggcg gttgctcccg gcgatcgcgt
    28561 tcgtcgaatc gggaggcgga aaggcttttc ggaacaacaa tattttcggc tgggacaacg
    28621 gaaactggcc ctttccgtcc attagagcga gcatccactt tgtcggcgaa cgcctgagcg
    28681 catcccaact atacgaacat aagaacgtag ctgaaaaact cgcaacgtac aacccacact
    28741 cgcattatcc gctccttgtc acatccgtta tggacgagat cggaccttca cagttcccac
    28801 cgacgaacta agtggattga gccgcagccc gtactccact tagtaacagc tctgatctct
    28861 atggatcaag ccgccggtac aaattcttgg ccgagggtcg gacgtttgag ccgtgccgcg
    28921 tcaacccgcc agccgacgga catgcccggt ccgccgcaca ggaccgcggg gcataataga
    28981 cagatgcaaa ccgcactggc gccagccggg gtagcggaag ggcgggcggc gccggaacga
    29041 ttcctgccgc ttgacgcgtt ccgcggcctg atcatggtcc tcctggtttc cgacgggttc
    29101 ggcctgcgcg ctctcgccaa acacccggtc ttccacggtc tcgcggaaca gtttgaacac
    29161 gtcccgtggg aaggagcggt gttctatgac acgattgcgc cggcgttcct gttcatggtc
    29221 gggatggccg tgccgtacgc actgggcctc cgcaccgagc ggggcgccac gttccgcgac
    29281 aacttgcggc atgttgccat gcgctcgctg cgcctgatgc tgtattccca ggtattgatc
    29341 tcgattcagt cgcaccggct gcactttcag atgcacaaca ttctgacgca catcgccgtg
    29401 gcgtacttcc tgtgcttcct gatcatgcaa tggccgttcc gctaccaggc gctggcggga
    29461 gtgggactgc tggcggcgca cacgtcgctg ttcttcttgt tccccggacc ggacggcccg
    29521 tttaccaaga ccggcaacat cggcgccgtc tgggaccgcg cgctgatggg gtacaactac
    29581 gcgtcctgga ccaccaacct gaatatgatc agcacaaccg ccagcgcgct attcggcgtg
    29641 tgggccggga acctgttacg gagcggccgc ccacgttccg agcagatgaa gattctgaca
    29701 gtggctatgg cggccgggtt cgccagcggg ctggcgcttt cgccttgggt gcccattatc
    29761 cggcgtatct ggacggcgtc cctcgcactg tacgccgtgg gttgggtgct actcatgttc
    29821 ctaacgctgt atgtgccgat tgaagtcggc ggttggcgta aactggcttt cccgttcgtc
    29881 gtggcgggct cgaacgcaat cttcatctac acgcttgaca tgatcctgcg gcggtggctc
    29941 gacgagtcgg tcgcggtgtt caccggaaag ttcacgttcc ttggcgcctt cggcccggta
    30001 gcacagagtt gcgcggtgct gctggtgatg tggtctctgt gctactggct ctaccgccac
    30061 aggatcttcc tcagactgtg agagcgccag gcggcagttg acgtcaccgg tggcaagtag
    30121 cataatcagc cggaattaaa aggaaattcc caggatacgc cgatggtaat tttgcgcttg
    30181 gcagggtggc gggttgccgc cgctgtgttt tggatcgcgg tggcttcgcc tgccgcgccg
    30241 cggccggata gcgatccggt actccatccc ggaccggagc aggtcccgga gtgggctcag
    30301 cagggcaagt atcggttcgc ccggctcgac gggggcccga ttgaaatcct caagaccgcc
    30361 cgctcctcct ggggcatgca cttcaccgca cgggagaagg agatcctcgg caacctttac
    30421 tccacctacc gcgaccggat gatcgaccta ctcgggcagg ccaacctgaa ctgggtctgg
    30481 atcacctgga gcgtgggcta ctcctgggag gacgaggccc ggcagcggga cgagtgccgc
    30541 acgctcacct ccaagctgca cgagaagggc attcgcgtgg cagcttacct gtgcgcggcg
    30601 agcatgttct gggagagcat gtttcgcgat gagccccgct ccacccggtg gctcaggttc
    30661 gacccgcagg gcgtgccttt ccgttactcc agcgggcgcg acccactgcg gttcgtcgcc
    30721 gacgtgtcca atcccgaatg ggtcgagtac cagaagcgcc gcatcggggc ggccatcgac
    30781 gcgggcgtgg acgcattctt cctcgacaac acttcccaga cagggtggtc ggataacgag
    30841 acgatggacc ggtacatcgg acgtttgcgc cgctacatcc gggaagagaa gcgttcgcca
    30901 gcggtgctgc tgaccaacta tggactggca tcgaaccgca tcgtgctgaa ccgcaacatg
    30961 gaggttgtgt tcaacgagta ctgggtggag ccgggtgtct tcgggcagga gtggaacgcc
    31021 accaacgtgc ggcggatgaa gtacgtccgc ggcgtggtgc cggagtggaa gcccctcatc
    31081 tccgagtatt cgcgtttcca cgagggggac cgtgctcacg gctggctgaa gccgaaatca
    31141 gcccagctcg ggatcgcgga ggccgcggcg ttcggatccg cttatgcgtg ggacatggaa
    31201 gggccgtttg atgcggctct cgtcgacggc gacgccggag cactcgagac ctggagggcg
    31261 atcggcagat acaacggctt tctgaaagat aacgaaaacc tttactggaa agcgcgcggc
    31321 gccgcacccg tggcagtatt gatctccgag cgtcggcgcc gcaacgtgat ccaatttgga
    31381 tgggacaaag atgagaccgg actgtacgat ttactggcca gcaacagcgt gcttcacgac
    31441 atccggttgg tcgacgcgct ggagggaaag caacttgcgg cctatgcggg agtagtagtc
    31501 cctccctttg ttgagtcctc ggcgcggttg acggacgtgc tgaagcgctt ccgaacgaat
    31561 ggcgggaagg tagtcacgct gagcgagacg ctgatcgaga gcctggagac gaacgagtcc
    31621 gcgcggcggg aggtgctgga aaaagttcgt gggctcgcac cagcccgaat cgggctggcg
    31681 gtagaaggtg tgccgcacgt gctcggcaac atcacgcggt taggttcccg aagggcgctc
    31741 gccgttcacc tgctcaacta cgcgccggaa gcagcttctg gcgtgcgggt gcgggtgaac
    31801 cttggccggg agtttgcgtt actcgccggc gcgcagccgc gcttgctcac gcccgatttg
    31861 cagaccgagg ggctggtcac agcgcggagc ggcgcctcca cggtggaatt tcagattcag
    31921 aatctggata cgtatggcgt catagtcatc gaatagcggg tcctaccgct ccgccaccag
    31981 atacccccgc accagcacct tcgatagccg tagcggggtg cccagcggcc cgggagtgac
    32041 gtccacgcac agccgggcgt cggaggcaac cagaaagccg gtcgtcatgc gagcgttcgc
    32101 gccacccacc ccgaagccgt tcagttgggc tgccgactcg aacaccttgc gccgtttccc
    32161 cggatccgcc aagttctcaa cgaacaaccg cagcacttgg acgatgcccg gcgttccgtc
    32221 aaagtagtac cagtcgacat cggtcgccac caatacccgg tcctccggga tacgaaacgg
    32281 ctctgcgccc gcaccgccgt ccgggaatgt gcggacaaaa tcgatctttg ccggaccgca
    32341 gccggtcctc acgcccgcaa ccatctcaag cagtacatgg tcgccggctg gcactccgag
    32401 gtgggttaac tcaccgccgg cgaggggaaa cgccaaaaac gcaagcacca gatagattct
    32461 cgacgtcatt cagccctcca ctcacacgat tttacggcca caaaggcgaa gagcgtgccg
    32521 gcttgccgac cggcacgctc tcacagggcc caaccaagaa accgagcaaa ggcagtcaat
    32581 ctaccgcgca gggatgggta gccaggcaac ctgagcgccc gccgagcgaa ctttcgccat
    32641 ctcgcgctcc gccttcgaag cgggtagctt atatccgctg gtcattccgg cagcccatac
    32701 gccttggagg aagaaggtgt tgccgtaacc gtggaacagc agtttgcccg tgtcagaagc
    32761 gggcgctttc gcagctgtgt agaccgagac aaatgcgccg gaggcgccgt cctcggttct
    32821 cagttccatc cggttgaagg cgggatcgac cgccacccgg tacactccgg ccgggaggac
    32881 ctggtccccc gcaatgaatc tgaacgggat ctcgatccgg accagcttgg tttcggcgca
    32941 aacgtcagcc accgacagca gggcaagcag cccggctgcg atcaggattc ttttcgagga
    33001 gtaattgttt ggcattgcgt tctctttccg gcctcttcag ccaccccgag aatacggctc
    33061 ttttctcccc ttggagatcg taagagattg tcggcaccac ggctggcgca ctttggcgtg
    33121 caatgacgag ctggtgtgga cttgtgccgc ctggggccac ggggaactcc ctgtttgcag
    33181 gggaagcggt cgatcacctt ccactcgtca aggcgcggca ctttggtgaa agcagcgact
    33241 gccagtcggg gcaatcggta ggaatactc
//
LOCUS       DATIFQ010000258         2959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1806834, whole genome shotgun sequence.
ACCESSION   DATIFQ010000258 DATIFQ010000000
VERSION     DATIFQ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806834"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VLE22_12145"
     CDS             complement(<1..797)
                     /locus_tag="VLE22_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HSB15202.1"
                     /translation="MRTSHIAPVVALALVCGGCRKAETQTKVLTAVKAAPVEAVAVSG
                     ETRYSANVVPDTSVNVAFRVGGYVTALHQVRGTGGDRALQEGDFVRRGTVLAVVRQDD
                     YRVKVELAKSTLAEATASLAAAKWQVSEAEASLRQTEIDFGRANSLFESQSLTKADHE
                     AAATKLEVAKARAASAKSQAESVQRRAAAAADQVKEAEIALGDTELRAPMDGTVLSRP
                     IEVGALVSPGSVGVVLGNTSSVKAAFGVPDAAVAQLRLGQALQIRTDA"
     gene            complement(800..2152)
                     /locus_tag="VLE22_12150"
     CDS             complement(800..2152)
                     /locus_tag="VLE22_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB15203.1"
                     /translation="MHIPPAVSGLVVFAVIAAQPAYCVQSVAPAETLTVERARTLALE
                     SNRDLKSARMEVAKAEDRLASARTRRLPSFKWSALGMQKITALDFSFPRGAFGTFEGI
                     GEVPATDTSIRSPRRPGALLIGQVNQPLSQQYRIGLQLGLLRAQRDSAVEQERAQRQA
                     VVAEVKRVYFSILQTQAALESAEEAIRLYRELDRVTGDFVLRQVALKAENLEVKTLLA
                     NAEYEALALRNPLETQKEQLNILLGRDVGTTFELGAVGEASDGETDLDAARRLALERR
                     PEVRQAALKLKQAEADRRAKRSEYIPDLSLSFNYFSPVNYGSLIPANIATVGLLFEWE
                     PFDWGRKRLEVAEKAKTVEQASLALRQTQAQVAVDVGSRFRKLREARQLLAVRVLARE
                     TAQENLRVANNRYKQQMILLKDVLQFQTSLAGANQNYQAALAAYWTARAEFEKALGEE
                     "
     gene            2364..>2959
                     /locus_tag="VLE22_12155"
     CDS             2364..>2959
                     /locus_tag="VLE22_12155"
                     /inference="COORDINATES: protein motif:HMM:NF019988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15204.1"
                     /translation="MKPRIVAAGTALPKNRFSQELLLEMAGYDHKRGAFFLNSDIQFR
                     HLYFEPGFGGNETIDQMNDRARRGAAEIGGEAIRRCLERAGAAPADVDFLATTTCTVS
                     MCPHLDTLFIRDLKMRPSIRRAHIGDTGCASAMVSLQAAWNHLHAYPDDCAVVAAVEI
                     CSAAYYRDGSIEAAIGEAIFADGAGAMYFANSGTGFEVL"
BASE COUNT          522 a          934 c          918 g          585 t
ORIGIN      
        1 gcatccgttc gaatttggag agcttggccg aggcggagct gggcgaccgc tgcgtccgga
       61 acgccgaatg ccgccttgac ggaactggtg ttgcccaaga ctacgccgac cgatcccggg
      121 ctgacaagag cccccacttc aatgggacgg ctgaggaccg ttccatccat cggcgctctc
      181 agttctgtgt ctcccagggc gatctcggcc tccttcacct ggtccgccgc ggcggccgcc
      241 cggcgttgca ccgactccgc ctggctcttg gcactggctg cccgcgcttt tgcgacttca
      301 agcttggtgg cggctgcttc gtgatcggcc ttggtcaggc tctggctttc gaagaggctg
      361 ttggcgcggc cgaagtcgat ctcggtctgg cgtagcgaag cctcggcttc ggacacttgc
      421 catttagcgg cggcgagaga agcggtggcc tccgcgagcg tcgacttggc gagctcaact
      481 ttcacgcggt agtcatcctg ccgtacgacc gcgagaacgg tccctctccg cacgaagtcg
      541 ccttcctgga gtgcccggtc gccgccggtc ccacgcacct ggtggagcgc ggtgacgtac
      601 ccaccaacac ggaaggcgac gttcacactc gtgtcaggaa cgacgttggc ggagtaccgc
      661 gtttcgccgg acacggcgac ggcttcaaca ggcgcagcct tcacggccgt cagaaccttc
      721 gtttgggtct ctgctttccg acaacccccg cataccaagg ccagagcgac gacgggtgcg
      781 atgtgagatg tacgcatcac tactcctctc ccaaggcctt ctcaaactcc gctcttgcgg
      841 tccagtaggc cgccaaggcc gcctgatagt tctggttagc gccggccaga gaagtctgga
      901 actggaggac gtccttaaga aggatcatct gctgcttata gcggttgttt gcgactctga
      961 ggttctcctg cgccgtttcg cgcgcgagca cgcgaaccgc cagtagttgg cgggcttcgc
     1021 ggagtttcct gaaccggctg cccacgtcga cggcgacttg cgcctgcgtc tggcgaagcg
     1081 caagactggc ctgctccacc gtcttcgcct tctccgcgac ctcgaggcgt ttccgccccc
     1141 agtcgaaggg ctcccattcg aatagcaacc cgacagtggc aatgttggcg ggaatcagtg
     1201 acccgtagtt gacaggtgag aagtagttga agctgagact caagtccgga atatactcgg
     1261 accgtttggc gcgccggtcc gcttcggctt gcttgagttt cagagccgcc tgccgaacct
     1321 cggggcgccg ttccagggcc aggcggcgcg cagcgtccaa gtccgtttcc ccatcgctcg
     1381 cctcgccgac cgcaccgagt tcgaaggttg tgccgacgtc gcggcctagc aggatattta
     1441 gctgctcttt ctgggtctcg agaggattcc gcagcgccag agcctcatac tcggcgttcg
     1501 ccaggagtgt cttaacctct agattctcgg ccttcagcgc gacctgcctc agtacgaagt
     1561 cgccggtgac gcggtccagt tcacggtaaa gccggatggc ttcctcggcg ctttcgagcg
     1621 cagcctgcgt ttgaagaatc gagaagtaga cgcgcttcac ctccgcgacg accgcctgac
     1681 gctgcgcgcg ttcctgctcg actgcgctgt cccgttgggc acggaggagg ccgagttgca
     1741 ggccgattct gtattgctgg gacaacggct gatttacctg gccgatcagc agggcccctg
     1801 gccttcgtgg tgatcggatg gacgtgtcgg tcgcaggcac ctccccgatt ccctcaaacg
     1861 tgccgaatgc gccgcgggga aaactgaaat cgagagccgt gatcttctgc atgccgaggg
     1921 cagaccattt gaacgatgga agacggcgag ttcgtgctga cgctagccgg tcttcagctt
     1981 tggccacttc catgcgcgcg ctcttgaggt cacgattgga ttccaaagcc agcgtgcgcg
     2041 cgcgctccac ggtcagagtt tcagcgggtg cgacactctg tacacaatac gcgggctgcg
     2101 cggcaatcac cgcgaacacg acgagtccag aaacggcggg tgggatgtgc acagcgcccc
     2161 tctacgctcg tacatccgaa actgtaacgg caatcagtat accactccat cgttgctacg
     2221 gataacggaa accggctggc aaattgtcgt caaacaccga gtttggcctc aatggaagtg
     2281 cgtcgcaggg gcggaccact cccgtcggtt atgcctcggc tagaagcagt tacaatgaga
     2341 ctgtttcaac tggggggaaa ttcgtgaagc cgagaatcgt agccgccggc accgcgctgc
     2401 cgaagaaccg cttttcccag gagttgctgc tcgagatggc gggatacgac cacaagcgcg
     2461 gcgcgttctt tctgaacagc gacatccagt tccgccacct ctatttcgag ccgggatttg
     2521 gcggcaacga gaccatcgac cagatgaacg atcgggcgcg ccgtggcgcc gcggagatcg
     2581 gaggggaggc gatccggcgg tgtctggaac gggccggcgc ggctccggcc gacgtggact
     2641 ttctcgccac caccacctgc accgtctcca tgtgcccgca cctcgatacg ctctttattc
     2701 gcgacctcaa gatgcgcccg tcgatccggc gtgcgcacat cggcgatacc ggatgcgcga
     2761 gcgcgatggt gtcacttcag gcggcatgga accatttgca cgcgtacccg gacgactgcg
     2821 ctgtggtcgc ggccgttgaa atctgctcgg cggcgtacta ccgggacggc agtatcgaag
     2881 cggccatcgg cgaggccatc ttcgccgacg gcgcaggcgc aatgtatttc gcaaactcgg
     2941 gtacgggttt cgaggtgct
//
LOCUS       DATIFQ010000259        26447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_467585, whole genome shotgun sequence.
ACCESSION   DATIFQ010000259 DATIFQ010000000
VERSION     DATIFQ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 26447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26447
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467585"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3049
                     /locus_tag="VLE22_12160"
     CDS             <1..3049
                     /locus_tag="VLE22_12160"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HSB15205.1"
                     /translation="LDRQTDYPATVAEFGRLLSEDGLRLDRLKDPWGSPVRVAISHFR
                     DRRIIEIRSPGPDRIYGNRDDCTVTTFSGSYFHELAAKINRVLGIGADFPTDEAQLRS
                     RISSAGVDLRTERDPWGRPYYPTLRTEATFSDRYQVYTYAEYQGVAEERKQVIPTKRR
                     FLVAEIHSSGEDGIANTYDDFVLVQFTRVITEDQQPHPAFPAPSSTLPSMSLGGRGTI
                     TGVISDPNGAVVPGAEVVLNDTYRTQTDDQGRYDFRGVPEGKYTVRVWLAGFKITVIG
                     GVPVQPDRVTRLDAVLSLGQVTETVEVAASPTVLETASASVASKVVSVNTLTSTPRLR
                     EYFPETLYWAPEVITDSDGRAVMKFKLADSITTWRVAVMASTTDGRIAEAATDIKAFQ
                     PFFVDLDPPQVLTVADEISLPVPIRNYLTSDQAVAVNITAPPALQLSGRPTRQERISA
                     NSSVNTAVVLRASATAPGARLRVTAQARGGNDAIEKPIAIHPDGESVSRAISDLLNDG
                     RLLRIDVPANAIPGSVRAEVKVYPNVLAHVIESMESLLRLPSGCAEQIISSSYSNLLF
                     LRALKISGLQDAKLEERARRNLEAGYRRLLSYRKEQGAFGYWSNSPADIAVTAYAVTF
                     LEDASKLIAVDQSVIDQARSYLSDQSPADLTVRSVALRAIAKADPKYETAVIKRLGEL
                     ARSAVEFEDPYTLSTFVLAALDAGRPELALPAVDRLRESARDEQGAAWWSLRRNTPYY
                     GWGRAGIIETTALAVTALAEWQAKKGSDPSLSALVDRGIVFLLRNKDSRGVWLTGQAT
                     VRVFEALIRVLAAPEEARASAVELQVNGLSAGRIPVPEGRSVRGPATVDVSRLIKPGM
                     ANEVSLAASGGRLAAQVQFAADWYQPWDQPRTFPDFSLDVRYATTEAGVNDIIRCDVT
                     VSRPAFRGYGMMIAEVGLPPGAEVDRSALSAVLDGNYGVGSFEVAPDRVVFYVWPRAA
                     DSKFSFTFRLRFPMRARTASSALYDYYNPEARVVLPPATFSVK"
     gene            3125..3733
                     /locus_tag="VLE22_12165"
     CDS             3125..3733
                     /locus_tag="VLE22_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSB15206.1"
                     /translation="MAGSIPVRAVARPEPFEFESWMKAEQKRVYLLCRRLLGDRDEAD
                     TATQEVFLKAYRSLSSSTAELDEPARWLTRVAVNTCLDRLRSRRWRFWRQRPEPEDEE
                     IIIAFTPAAGPDAEDELFARQIAVRLNRALEGLSPRQRSVFTLRHFEDCSLEEIGATL
                     GLDLGTVKVHLSRSLAKLRKELKDLYLMAGPASARRRPAVAP"
     gene            3730..4173
                     /locus_tag="VLE22_12170"
     CDS             3730..4173
                     /locus_tag="VLE22_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15207.1"
                     /translation="MTERTWMNGRHWTDDELIARLYGVGPEDGHIDTCEDCARRLSAI
                     KAVREGVLNSPLDVSEAFLAAQRRAVMERAHGGNYGSWVFSLTPALSALALILLAFLL
                     SRPAPPPQPSLASSETQLFVEVYEEIQTAEPSAVAPIYGLFEVGQ"
     gene            4170..4661
                     /locus_tag="VLE22_12175"
     CDS             4170..4661
                     /locus_tag="VLE22_12175"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HSB15208.1"
                     /translation="MKKLVMLAFIPVTMLLGQGPGMGMFPWWDSPLAQDMNLSESQKQ
                     QIKNTVRDYRDRLIEQRAALQMAEGNLQDSLNEDQVNEAKANEAIEKTVAARGDLART
                     ISQMSLKLRMILTPQQWQELQKRRPMDRFRERGGFRGRRPGRQGAPPEPGHSPDAPPP
                     GTF"
     gene            4750..5679
                     /gene="nadA"
                     /locus_tag="VLE22_12180"
     CDS             4750..5679
                     /gene="nadA"
                     /locus_tag="VLE22_12180"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSB15209.1"
                     /translation="MPVITTIAEEILELKEERRAILLAHHYQESEIQELADCIGDSLE
                     LARKAREFDGDVIAFCGVWFMAETAKVLNPSRIVVVPDAEASCSLVESCPVEPIRAYR
                     QKYPDHVIVSYINTSVDVKAESDILCTSRNAVDVVNSIPPDKTVLFLPDRNLGNYVKQ
                     QTGRENMKIWQGTCVVHATFASRRLAAARADHPGALVAAHPECPEDVLAMADFIGSTS
                     AIINWCVNSDATEFIVMTESGVRHSLEKLAPQKSYYFVPNEQCNCSECPYMKRNTLEK
                     LRDCLRDLRPTVEIPEDTMRRALLPLERMLSIK"
     gene            5687..6688
                     /gene="moaA"
                     /locus_tag="VLE22_12185"
     CDS             5687..6688
                     /gene="moaA"
                     /locus_tag="VLE22_12185"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HSB15210.1"
                     /translation="MGNPAQLRDTFGRLHDNLRVSVTDRCNIRCYYCMPEHEVQFVPR
                     EEILTFEEIERFVRMAVSLGIRKVRVTGGEPLVRRNLHLLIRKLAAIPGLTDLALTTN
                     GVLLREQAEPLYEAGLRRINIHLDTLDRNRFLRITRRDDFDRVVEGIETCRRLGYDPI
                     KLNAVAVKDLTEPDVVPLARFGREKNIEIRFIEFMPLDAQGLWLRDKVLLADDIVQTI
                     SSEVCPLIEIPDRDPRAPATEYRYADGGGTVGFIASISKPFCLNCNRIRLTADGKLRY
                     CLFAIEETDVKGLLRGAATEEEIARIIAEVVAAKWEGHEVNTARFVAPPRPMYAIGG"
     gene            6762..8411
                     /locus_tag="VLE22_12190"
     CDS             6762..8411
                     /locus_tag="VLE22_12190"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HSB15211.1"
                     /translation="MRIALGQINTTVGDLAGNVDLMVQAARRAAADGAELIVFPELSL
                     TGYPPRDLVEKPSFLERTETQLDRLAAETAALDIALVCGYVGRSNSTSGKNATNSAAV
                     LENGRIVFRQVKMLLPTYDVFDEARYFVPAEHQLLLELRGRKIALTICEDAWNDKQFW
                     ERRLYRRDPVEELVEQGAGVLLSINASPYHMNKRELRREIFAATARRHGLPLVYVNQV
                     GGNDQLVFDGSSFAMDKDARVIASAASFAEDLVFVDTMSGEGDLHENLADEQEAVYEA
                     LVLGTRDYIRKCGFRKVLLGLSGGIDSSLTAVIAADAVGPENVIGVAMPGPYSSEHSL
                     RDARALAERLGIRCDTTSITGVYDKFLDVLDPLFQGAPRDVTEENLQARLRGVTLMAL
                     SNKLGALVLTTGNKSELAVGYCTLYGDMIGGLAVISDVPKTLVYELSRVANCRHPCAI
                     PESVFTKPPSAELRPNQKDSDSLPEYAVLDVILRDYVEEYKTPAWISENRGFPLELVR
                     DVVNKVDRNEYKRQQAAPGLKVTSKAFGIGRRFPIAQRFLE"
     gene            8408..10909
                     /locus_tag="VLE22_12195"
     CDS             8408..10909
                     /locus_tag="VLE22_12195"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSB15212.1"
                     /translation="MRLVLITLPVALAALLSSQPAPREQVGPLPDGGFLLNSGWRLSP
                     AGKQVALDTFPMSMALTPDGKHLLVLNGGYKPPSISVIDTASAAETGRTPVPDAWLGL
                     TFTPKGDLVYIGGGSQAAVLEFSFVEGRLTPTRTFAVVAEDKRTHQDFIGDVALSPDG
                     RLLYAADLYRDSVVVINPQSGIVIERIKTGRRPYRILFHPDGKSFFVSSWTDGSVYHH
                     ESEKGTLLGRVRIASHPTDMVWRPGRPEASEQDEAAPPYVARVFVAAANTNSVYVIGV
                     SEGKDLQVVESINVSLTPRQPAGMTPSALALSKDGKRLFVACSDANAVAVVDVSEPRS
                     RVLGFVPTGWYPTTVRALADGRLVILNGKGLRSYPNPRGPNPLARPEAAHLGPVRPEQ
                     VQYVGHLQTGTASFVDPFDDERLEAHTRTVLSNSPYRDSKLDDAGTGKDNPIPAFPGG
                     TTPIEHVIYIVKENRTYDQVLGDLKQGNGDPSLVLFGEDVTPNHHKLAREFVLLDNFY
                     VNSDVSADGHNWTTAAIAPDYVQKLWPNSYAKRRRHYDYEGQEAAAAPPAGYLWTNAA
                     GAEIPMRNYGYFVSNRPKPGADGIQVDAVRDPVLRRVTNLHYRGFDLDYPDVERAKVF
                     LRDLADFEKAGAMPRLLLLRLGNDHTSGASPGKLAPKSAVADNDYALGMIVEAVTKSS
                     FWPKTAIFVLQDDAQNGADHVDSHRSPAFVISPFAKRGIVDGTMYNTTSMLRTIELIL
                     GLQPMTHFDAGARPMSAMFQTTPNLKPYSAESPRIPLNERNPAVSPAAARSARLDFSE
                     EDRIDDDELNEILWVAVRGSEGAPPPAPVRSFFSR"
     gene            10915..13056
                     /locus_tag="VLE22_12200"
     CDS             10915..13056
                     /locus_tag="VLE22_12200"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HSB15213.1"
                     /translation="MPRIYLCFLWHMHQPFYKDLFSQEYRLPWTRLHALKDYFGMVDV
                     LRDFPEVKQTFNLVPSLLLQVEEYASGEAADPFLRCALKPAEALTDAEQEFILRYFFQ
                     ANPSRLIYRFKRYGELYDAWLASDRDVKRARRFFSSQAFRDLQVLSQLAWFDEKFLEH
                     DSTVAELVRKGRDYTLDDQELMGRKQTEILGKVLPVYREFASKGQIEISTTPFYHPIL
                     PLLCDSDIAGEAHPHVPLPSRFRYPQDARMQLERSRQFIRDRFRRYPRGLWPSEGSVS
                     DEVFQIASELGFTWAATDNGVLARTLGDSPGVEGTYRPYLWRQGKREIHVIFRDHFLS
                     DLVGFVYSRMGPGEAAADLLQRIRENCRGILNSGRDALVPVILDGENAWEYYERNGRP
                     FLRELYRRIMEDRQIRAVTIHEALKLIEPQPIDRISPGSWINANFDVWIGAEEDNTAW
                     EYLLRARQTYDRVASGSHGDAPWDGARLKLAEEELLIAEGSDWCWWYGPEHDSANRPE
                     FDQLYRDHLANAYRALGQAPPEELSRTILKVAEAVYHHPPTGPVRAAIDGEETSFFEW
                     LGAGVYRVDSRSGAMHGHRFLIRELHYGSDGPNFYMRLDLEPVSLEGMEARINIRPWT
                     GDSPASCMRLRFEQGTAVTVEQQFAAGGEAEVAFHRVLEIRIPLMAASLVQGQPVALQ
                     VSIWEDSLPLDAVPHQGWLECSTAEPTDWPV"
     gene            13183..14997
                     /locus_tag="VLE22_12205"
     CDS             13183..14997
                     /locus_tag="VLE22_12205"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HSB15214.1"
                     /translation="MLLSCGLTVTVALAVLVGCGPLTKKAEVGGELKQWHKVTLTFDG
                     PRTAEDATPNPFRNYRLNVTFTHGQTGTSVTVPGYYAADGHAAESSATSGGKWRVHFR
                     PGQQGEWSYLASFRTGQDVAIDPDANAGQPAAFDGATGTFVISATDKTGVDLRAKGLL
                     QYAGGHHLRFAGNGEYFLKGGADSPENFLAYAEFDGTYDADADSGSYKKVGPFIHRYE
                     PHIRDWRDGDPTWKGGKGKGIVGALNYLAEQGVNSVYFLTYNIDGGDGRDTWMWTGPN
                     VRDRFDASRLDQWEIVFDHMDRKGIMLHVVTQETENDRALGGGPGLNPIRKLYYRELC
                     ARYSHHPALVWNLGEENNTSDRDRKEVAAYIRSLDAWRHPITVHTHAGQALKFYNGLL
                     GDGDFEATSIQGALKDYNRDAIVLRKRSAEAGRKWVIFGDEQNPADRGVLPDFADPDH
                     NEPRIQALWGNLMGGGSGVEWYFGFAFPHMDINCEDFRSRERMWSQTRYALEFFRKHL
                     PFWEMEPDNSLASGAKDARVLAGGDAIYAVQLTAGGNAALKLGSGTYTVRWYDPRAGG
                     ELQAGTVDTIRGPGVKSIGRPPSNALRDWIALVKRAAD"
     gene            15034..15594
                     /locus_tag="VLE22_12210"
     CDS             15034..15594
                     /locus_tag="VLE22_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HSB15215.1"
                     /translation="MTRKVAAGGGLELVLPQCEKGIGAIVRKADSPDLISGVRVVSHN
                     LWPDDRGYFMEILRVGHGLPAGFPPAETQVSLALGYPGTVKAFHYHLHQTDCWAPAAG
                     MFQVALVDLRKGSPTFGVRNTLYAGSLWPVQILIPPGVGHGYKIIGVDPGVLVYVTDH
                     FYNPDDEGRIPYNDPAIHYDWETQHK"
     gene            complement(15632..17323)
                     /locus_tag="VLE22_12215"
     CDS             complement(15632..17323)
                     /locus_tag="VLE22_12215"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HSB15216.1"
                     /translation="MIAAAGLLLSLPVLLQGPLPNAKDFANHLARYLQFREQFWSGDF
                     YPRWLQGANAGLGSPVFFVYGPFFYYFASLFYPLCQLLFGSVEHFEFRLSLAVAMMAS
                     GVATYRWLRLLTSRTGAFAGAALYMAAPYHLWADLYVRCAAAELWAFVWLPTILYALE
                     RIAISRRSGILLMAVGATMLLVTHILSVALLLPFVAGYVLTFMQSHVRRTVFAGAIAG
                     TALASLLSSFYLLPALLYEQNISVERFTSGPHYYYKDNFLVTDSSLFVWDAAGNPFKW
                     YATWIAIALTLICLFSFLLCLNRGSRRNRLAVFAVVSAAVSLYLMTPWSAGVWDALPM
                     LQGLQFPWRFLLVLSAVSSLLFALAVGREGMAREPWGRLVEFALLLIAVASCALPSAH
                     ILWLYGKKAAMTPEYELLLRSRGDDLTRAWARWSDPETLEPAGLLRLSRETPPVVVAG
                     GMGSGRIKRLGPREVQVFTDLSEPATVQIRQLYYPGWIVRVAGESASSPAGPAPRSGL
                     TQFIAPAGRHELLVSLPKSTPERLGEVMSGIGLLGCAILLIGGGASRTLGRLFRA"
     gene            complement(17418..18542)
                     /locus_tag="VLE22_12220"
     CDS             complement(17418..18542)
                     /locus_tag="VLE22_12220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB15217.1"
                     /translation="MRLESIAYLLDCSPATWDTKAEFHWRLCRRMRASGVQPILVFSE
                     ELPAPITERFTDAGAVVRACAFDRSRLGYYRQIGALVQQYRVGLVHVRYFAYFSAIPW
                     LARLQGVPHIVYTEANSGEWAADSRRAHLVRLRARLACQPISRFTAISEFIKHRLIRV
                     GIAEAKIDVVYNGVDTGRFVPDPGARERLARDLPIAPGDFVIAGAFSLLPWKQPAVLL
                     DACALLARRGVPVRLLVAGAGPLLTELQARSLKLGLAERTHWLGHSSGLETVLQACDV
                     FVLPSVGEAFGNVLAEAMACGVPVVASRSGGIPEVVEDGVTGRLVDPSNPAAFADAIE
                     RLACDTSLRREMGRCAIDRARQRFTVESAVEGTVRVYEGL"
     gene            18626..19237
                     /locus_tag="VLE22_12225"
     CDS             18626..19237
                     /locus_tag="VLE22_12225"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HSB15218.1"
                     /translation="MKQLLRGVVRILFYSMAALGLVWTLATVTPIDYHWATWLAGPWD
                     DPAADVLIVLGGSVLDDGTIGQSSYWRSVYAVRAFREGDVRTVVICGRRASVAMREFL
                     ESQSVPRSAIRIENRSSSTRENALFAREVLADEPGRKVLLTSDYHMFRARRVFQKAGI
                     DVLPRPIPDVRKQVGSWRARWPAFLDLVEETIKIGYYFARGWI"
     gene            complement(19246..20364)
                     /locus_tag="VLE22_12230"
     CDS             complement(19246..20364)
                     /locus_tag="VLE22_12230"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB15219.1"
                     /translation="MEILFWTFAGVVFYVCGGYFLLVTLLARLFPRPVKKSACEPAVS
                     LLVAAYNEAHVIEAKIRNALALDYPADRLEIVIASDGSTDGTPELVARHADGNRVRLL
                     RYPLNRGKLAVLNDSVPQLRGEIVAFSDAATMLEPGALRRLVANFADPAVGAVSGLYR
                     VVKHDSVNIGPPEDVYWKYETFLKRQEATLGSIIGAHGALYAIRKELYPFPAPDTIND
                     DYVIPIRILRKGYRIAYEPSAVALEEAHEMGGFGRRVRIMAGNLEQLRELKELLMPPQ
                     WLALFFFVSHKTGRLAAPLCLIGMAAATAPLLGRPLYQFAAGLQAVFYLLAVLGSFVR
                     LRPKFLRLPYYFCMINAAVFAGMYFVISGRRRMAWKRR"
     gene            complement(20374..21564)
                     /locus_tag="VLE22_12235"
     CDS             complement(20374..21564)
                     /locus_tag="VLE22_12235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB15220.1"
                     /translation="MKILWIKADFLHPTTKGGHIRTLEMLRRLHQRHEIHYVGFAEAG
                     ETEGPRRSAEYSTRAYPVSHRPAHKDSTAFLAQAAAGLFSSLPVAISRYRSSRMARQI
                     EELRTAERFDQIVCDFLTPAVNVRDLSDCVLFQHNVETVIWRRRAALTADPIRRWYFR
                     KQAEKMLRYEGEACRSARHVIAVSETDACLMRTLFGATRVSVAPTGVDVDFFRPPESS
                     ATVSDLVFVGSMDWSPNVEGVLYFVREVLPLIRRRRPECSLAIVGRTPRDEIATLPRK
                     DPWIRVTGTVADIRPYLWGSSVSIVPLRIGGGTRLKVYEAMAAKTAVVSTSVGAEGLD
                     VSSPENIRLADDPEAFAEQCLALLEDEGERTRMAQAAWEHVAGRFSWDRVASSFERIL
                     ESAA"
     gene            complement(21561..23492)
                     /gene="asnB"
                     /locus_tag="VLE22_12240"
     CDS             complement(21561..23492)
                     /gene="asnB"
                     /locus_tag="VLE22_12240"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HSB15221.1"
                     /translation="MCGIAGYVTFSPPAGPDSVLRRMTDSIHHRGPDGDGYFSDEFAS
                     LGHRRLSIVDLATGAQPMTNESGNLQIVYNGEIFNHADIRKELERSGHMYRSRSDTET
                     ILHAYEEWGPGCVDRFRGMFAFAIWDRTTRTLFCARDRLGIKPFYYFWNGRLFVFASE
                     IKALLEHPAISPEFDESLLSEHLAFGYSSGERTLFAGIRKLMPGHRLRLNAEGPAIDR
                     YWDVPRTGTSVRRDDGEWIAECRRRLEETVRMRLMSDVPLGMFLSGGVDSSAIAALMA
                     RMVSGRVKTYAVGYREEAFSELGYARQVARRIGTEHRDVIVSMEDFFNALPKLVWHED
                     EPITWPSSVSLHFLSRLASEEVKVVLTGEGSDEMFAGYSRYRFYLLNRSLMGFYRLLP
                     ARLRRLVASSIADSRFLSASQRRKLQHSFLGRGESIESLYLDNFYSAFPQAEQAELFS
                     DAGRAAGAYDSFLGYWDEGDGHSPLARMLYADQTTYLVELLMKQDQMSMATSIESRVP
                     FLDHQFVEFAAAVPDAMKIRRGTGKYILKKAVEDLLPGDIVYRKKMGFPTPLRQWLRR
                     PEAERLLAMLTSKDGLVASLVDTRALGELLDRHRRDIEDVTDRIWRLLNLQIWGEIFL
                     AGRRELRWEGLMAQAPAKR"
     gene            complement(23497..24789)
                     /locus_tag="VLE22_12245"
     CDS             complement(23497..24789)
                     /locus_tag="VLE22_12245"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSB15222.1"
                     /translation="MLVFLFLATGRLADFAGHQLRLPLILGTLAVLLALINGALARAL
                     KSRPGQLLTAFTLWMVLITPLSYWRGGSVNLLANQWLKSIMVYVMIAGLAQTLRQCRQ
                     VMNTLAVAVLAAALIVLYFGTLVAGRLAAPVGMLENPNDLAQILLLGLPFWYLLLIEK
                     SRVPFRRLLAALCAAPVLYVFLQTGSRGGLVAFAAMCAVIFVHASAAGRFKLMLVCMV
                     IAITVLVTTPEAVTARLAVTLKPEVTEQGFLDSYQMRAIESSQQRLSLFKESVELTIR
                     NPLFGVGPGQFQSVQAKKAGEEGERAMWRETHCMYTQLSSENGLPGLFLYAAALFFCV
                     RSANRIYKSTRNRPETADIAAMSYAILLSLIGFAATALFSSVAYHMFFPTLAGLSTAF
                     ARAVERELAARGVPNVLPAHSRRSTAVPSPAGKATARA"
     gene            complement(24873..>26447)
                     /gene="asnB"
                     /locus_tag="VLE22_12250"
     CDS             complement(24873..>26447)
                     /gene="asnB"
                     /locus_tag="VLE22_12250"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HSB15223.1"
                     /translation="DTETLVHLYEDAGADAVSRLRGMFAFALWDAKRRRLLLARDRFG
                     KKPLYYAVLASGLYFASELKCLRAAGVPLELDPEALRLYFQFNYVPDPYSPFQAIRKL
                     MPGTWLEYGADGSCRQQRYWRLPPPAEKVAPRMSEEDAAKRLREQFDEAVRIRMVADV
                     PLGAFLSGGIDSSLVVASMALQSSEPVKTFSIGFEEAEYNELEFASLVAKRYGTEHHQ
                     IIVRPDSVDLATRLACDFDEPFADSSAIPTFIVSEFARKHVKVALSGDGGDELFAGYD
                     SFRVIEKLRWWDSVPRVMRRFLAFAADRLPYSAYGKNYLRMASRPSALERYFENNYSP
                     YYLRRQLLKPEWMPPADLAYLTQTFPDCLLPSGADVLTQAMYFEATANLTGDMLVKVD
                     RMSMANSLEVRCPLLDQNLAELASAIPHGWKLRNGAGKRILLRALGDRLPPEVLRRRK
                     MGFGVPLAPWFRGSLRGFLREHVTSSQFLNRGIVSPEFVRQLLEEHDSGRRDNHHWLW
                     SLLMLELWFRDLRQTA"
BASE COUNT         5286 a         8326 c         8017 g         4818 t
ORIGIN      
        1 gctcgaccgc cagaccgact atccagctac ggtcgccgaa ttcgggcgtc tgctgtcaga
       61 agatggctta aggctggacc gcctcaagga tccgtgggga agtcccgtcc gcgtcgcaat
      121 ctcgcacttt cgcgatcgca ggatcatcga gatccggagt cccggccctg accggatcta
      181 cggcaaccgc gacgactgca ctgtgactac tttctcaggc agttacttcc atgagctggc
      241 cgcgaagatt aaccgagtgc tgggcattgg agcagacttc cccaccgacg aggcccagct
      301 ccgatcgcgt atttcctcgg ctggagtcga tctcagaaca gaacgcgatc cttggggccg
      361 accttactac ccgacgctca ggacggaggc cactttcagc gatcgatatc aggtctacac
      421 atacgcggag tatcagggcg tcgcggagga gcggaagcag gtgattccta cgaagcgacg
      481 attcctggtc gcggaaatcc atagctccgg agaggacggt atcgcaaaca cctatgacga
      541 tttcgtcctg gttcagttca ctcgggttat tacggaggac cagcaacccc atcccgcgtt
      601 tccggcacca tcttcaactc tgccttcgat gagcttgggt gggaggggca cgatcaccgg
      661 ggtcatttcc gatccaaacg gggccgtcgt acctggggca gaggtcgttc ttaacgacac
      721 gtaccggaca caaacagacg atcagggccg gtatgacttc cgaggtgtcc cggaggggaa
      781 gtacacagtc agggtttggt tggccggttt taagatcacc gtgatcggtg gcgttcccgt
      841 ccaacccgat cgcgtcacac gattggatgc cgtattgagc ctcggtcagg tgaccgaaac
      901 agtcgaagtg gcggcctccc caaccgtcct cgagacagca agtgcctcgg tagcctctaa
      961 ggtcgtgtct gtgaatactc tgacgtcgac tcctcgactg cgcgaatatt ttcccgaaac
     1021 gctgtactgg gcgcccgaag tgatcaccga ttctgacggc cgcgccgtaa tgaagttcaa
     1081 gctggccgat tcgattacga cctggcgtgt ggctgtgatg gcgtccacta cggacgggcg
     1141 catcgctgaa gccgccacgg atatcaaagc gtttcagccg ttctttgtcg atctcgatcc
     1201 gccccaggtc cttaccgtag ccgacgaaat ctctctgcct gtacccattc gaaattatct
     1261 gacttccgac caagccgtcg cggtcaacat tactgctccc ccggctctcc aactgtccgg
     1321 acgaccgacg cggcaggaac gcataagcgc caactcgtcc gtaaataccg ctgtcgtact
     1381 acgggcgtcc gcaacagcgc ccggtgcgcg tctgcgcgtg acggctcaag cgcgaggagg
     1441 caacgacgct attgagaagc cgatcgcaat tcatccagac ggagagtccg tttcgcgagc
     1501 catcagcgac cttctgaacg acggccgact gctgcgtatc gacgttccag ctaatgcgat
     1561 cccggggagt gttcgcgccg aagtcaaggt ttacccgaac gttctggctc atgtgatcga
     1621 atcgatggag tccctcttgc gtttgccgtc gggctgcgcg gaacagatca tttcttcctc
     1681 ctactccaat ctgttgttct tgcgtgcgct aaagatctct ggccttcagg atgccaagct
     1741 ggaggagcga gcgcgccgca acctcgaagc cggataccgg cgattactga gctaccggaa
     1801 ggagcaaggt gctttcggtt actggtccaa ctcgcccgcg gacatcgcgg tcacggcata
     1861 cgccgtgact ttcctcgaag acgcgtcgaa gctgatcgct gtcgaccagt cggttatcga
     1921 ccaggcgaga tcctacttga gcgaccagtc tcctgccgac ttgaccgtcc gctccgtcgc
     1981 gttgcgggcg atcgcgaagg cggacccgaa gtatgaaacc gccgtgatca agcgcttggg
     2041 tgagcttgcg cggtccgctg tagagttcga agatccatac accctgtcca ccttcgttct
     2101 ggctgccctg gacgcaggcc ggcccgagtt agccttacct gctgtcgaca gattgcgcga
     2161 gtccgcacgg gatgaacagg gcgccgcgtg gtggtcgctc cgcaggaaca ctccctacta
     2221 cggctggggt cgcgccggca tcattgagac gaccgctctc gccgtgacgg ccctcgccga
     2281 atggcaagcc aagaaaggca gcgatccaag tctgtcggcg ttggttgacc gtggcattgt
     2341 gttcctcctg cgcaacaagg atagccgtgg agtatggctt accggccaag ccacggttcg
     2401 tgtctttgag gcactgattc gtgtactcgc cgccccggaa gaagctaggg cttccgctgt
     2461 ggaacttcag gtcaacggct tgtcagccgg ccgaattcca gtacccgagg gccgaagcgt
     2521 gagagggcca gcaactgtcg acgtttcgcg cctgatcaaa cctgggatgg ccaacgaggt
     2581 ctcgctcgca gcgtccggcg gccgtctagc cgcgcaagtt cagttcgcgg ccgactggta
     2641 tcagccatgg gaccagccac gcacctttcc ggatttcagc ttggacgtaa gatatgccac
     2701 cacggaagcc ggcgtgaacg acataatccg gtgcgacgtg accgtctcgc gcccagcatt
     2761 ccgaggctac ggcatgatga tcgcggaagt gggattgcca cctggagccg aggtggatcg
     2821 atctgcgctt tcggccgtgc tggatggcaa ctacggcgtg ggatcgttcg aagtcgcacc
     2881 cgaccgcgtg gtcttctatg tctggccgcg tgctgctgat tccaaattca gcttcacctt
     2941 ccggctgcga tttcccatgc gggctcgtac ggcgtcctca gcactgtatg actactacaa
     3001 cccggaggct cgggtagtgc tcccgccggc gacgttcagt gtgaagtagg caggggcgcc
     3061 aagtaggctg gtcagatgta aacctttccc ggccaacgtc gtcatgaacg gtatgatggg
     3121 cgtgatggcc ggaagcatcc ccgtgcgagc cgtcgcccgt cccgagcctt tcgaattcga
     3181 gtcctggatg aaagccgagc agaagcgggt ctatctgctt tgccggcgcc tcctcggcga
     3241 tcgggacgag gccgataccg ccactcagga ggtgtttctc aaggcgtatc gctctctcag
     3301 cagcagtacc gcggaattgg atgaaccggc gcgatggctg acgcgggtgg cggtaaacac
     3361 gtgtctcgac cggctaagat cgaggcggtg gcggttctgg agacagaggc cggagcccga
     3421 agatgaggag atcattatcg ccttcacgcc ggccgccgga ccggatgcgg aagacgagct
     3481 gttcgctcgt cagatcgccg tccggttgaa ccgggcgctc gaaggcttat cgccccgtca
     3541 gcgctcggtg ttcacgttaa ggcacttcga agactgcagt ctcgaggaga tcggagcgac
     3601 cctggggctc gacctcggaa cggtgaaggt gcatctttcg cggtcgttgg caaaactccg
     3661 taaggaactg aaggatttat acttaatggc tggccccgca tccgcgcgga gacggccggc
     3721 ggtggcgcca tgacggaaag aacttggatg aatggcaggc attggacgga cgatgaactg
     3781 atcgcccgcc tgtacggcgt cggccccgag gacgggcaca tcgacacgtg tgaagactgc
     3841 gcccgccgtt tgagcgcgat caaggccgtg cgggagggcg tgctgaattc accgctggat
     3901 gtttcggagg cgtttctcgc ggcccagcgg cgcgcggtaa tggagcgtgc acacggcggc
     3961 aactacggtt cctgggtatt ctcactcacc ccggcgttga gcgccctggc tctcattctg
     4021 ctggcgtttc tgttgtcgcg gccggctcct cctccgcaac cgtcactggc ctcctcggaa
     4081 acgcagttgt tcgttgaggt gtatgaggag attcagacgg ccgaaccgtc ggcagtggct
     4141 ccgatttacg gtctttttga ggtagggcaa tgaaaaaact tgtgatgctg gccttcatcc
     4201 cggttacgat gctgctgggt caaggaccgg gcatgggtat gttcccatgg tgggacagcc
     4261 cgctcgcgca ggacatgaat ctgtccgaga gccagaagca acaaatcaag aacacggtcc
     4321 gcgactaccg ggaccgcctc atcgagcaac gagccgcgct ccagatggcg gagggcaacc
     4381 ttcaggactc tctgaatgag gaccaggtca acgaggccaa ggcgaacgag gcgatcgaga
     4441 agaccgtcgc cgcccggggc gacctcgcgc ggacgatctc gcagatgagc ctgaagctgc
     4501 gcatgattct cactccgcag cagtggcagg agttgcagaa gcgccgtccg atggaccgat
     4561 tcagagagcg gggaggattc cgcgggcgca gaccgggcag gcaaggagcg ccaccggaac
     4621 cgggacattc acccgacgcg cctccgcccg gtaccttctg acgcacctgc gcgagcgggc
     4681 gcaactcgcc caatcgtacc ctgcaatcac agccggggtt gttacaataa gggaaaagcc
     4741 ctctttgcca tgccggttat caccaccatc gccgaagaga tcctggaact caaggaagag
     4801 cgccgggcga ttctgctcgc ccaccattac caggaatcgg aaatccagga actggccgac
     4861 tgtatcgggg acagcctgga actggcacgc aaggcccgcg agttcgacgg agacgtgatc
     4921 gccttctgcg gggtgtggtt catggccgag acggccaaag ttctgaaccc cagccgcatc
     4981 gtggtggtgc ccgacgccga ggcgagctgc agcctggtgg agtcctgtcc ggtggaaccg
     5041 atccgggctt accgccagaa gtatccggat cacgtgatcg tcagctacat caacacgtcg
     5101 gtagacgtga aggcggagag cgacatcctc tgtacgtccc gaaacgccgt cgacgtcgtc
     5161 aactcgatcc cgccggataa aaccgtcctg ttcctcccgg accgaaacct cggcaactac
     5221 gtcaagcagc aaacgggccg cgagaacatg aagatctggc agggcacgtg cgtggttcat
     5281 gcgacgttcg cgtcccggcg cctggcagcg gcccgagccg accacccagg cgcgctcgtc
     5341 gcggcgcatc cggaatgccc ggaggatgtg ctggcaatgg cggatttcat cggctcgacg
     5401 tcagccatca tcaactggtg cgtgaacagc gacgccaccg agttcatcgt catgaccgag
     5461 agtggcgtgc ggcactccct tgagaagctg gccccgcaga agagctacta cttcgttccc
     5521 aacgagcagt gcaattgcag cgagtgcccg tacatgaagc gcaacacgct ggagaagctt
     5581 cgcgactgcc tgcgcgacct gcgtcctacc gtggaaatcc cggaggacac catgcggcgc
     5641 gctcttcttc cgctggagag aatgctctct atcaagtaaa ccccgcatgg gcaaccctgc
     5701 tcagctccgc gatacgttcg ggcgactgca cgacaacctc cgcgtcagcg tgaccgaccg
     5761 gtgcaatatc cgctgctatt actgcatgcc ggagcacgag gtgcagtttg ttccccggga
     5821 agagattctc actttcgagg agattgagcg ctttgttcgt atggcggtgt cgctgggtat
     5881 ccgcaaagtc cgtgtcaccg gaggagagcc gctagtccgc cggaaccttc acttgctgat
     5941 ccgaaaactg gccgcgattc ccggcctcac cgacctggcg ttgaccacca acggcgtcct
     6001 gctccgggag caggccgaac ccttgtacga agccgggctg aggcgaatca acatccacct
     6061 cgacacgctc gaccgcaatc gcttcctgcg cattacgcgc cgtgacgatt ttgaccgggt
     6121 cgtcgagggc atcgagacct gccggcgtct gggctacgac cccatcaagc tgaacgccgt
     6181 ggccgtcaag gacctgacgg agcctgacgt cgtgccgctg gcgcgtttcg gccgcgagaa
     6241 gaacatcgag atccgcttca tcgagttcat gccgctcgat gcgcaaggtc tctggctccg
     6301 ggacaaggtc ctgctggccg acgacattgt acagacgatt tcgagcgagg tctgcccgct
     6361 gatcgagatc cccgatcgcg atccgcgggc gccggccacc gaataccgat acgccgacgg
     6421 cggcggcacg gtggggttca tcgcctcgat cagtaagccg ttctgcctga attgcaaccg
     6481 catccgcctt accgccgacg ggaaactccg ctactgcctc ttcgccatcg aagagacgga
     6541 cgtcaagggc cttctgcgag gcgcggcgac ggaagaggag atcgcacgca tcatcgccga
     6601 agtggtggct gccaagtggg aagggcacga ggtcaacacc gcccgattcg ttgcgccgcc
     6661 caggccgatg tacgcgatcg ggggctgacc gctccggccg ctccggcgac tggtggccct
     6721 cgcgaagtgt cccgaacgcg ctttgtacaa tgagagattc catgcgtatc gccctggggc
     6781 agatcaacac cactgttggc gacctggccg ggaatgtcga cttgatggtt caggcggccc
     6841 gccgggcggc cgccgacgga gcggaattga tcgtattccc ggagctgagc ctcaccggct
     6901 atccgccccg agacctcgtg gagaagccga gcttcctcga gcgcaccgaa acgcagctag
     6961 accggctggc ggcggagaca gcagcgctcg acattgcgct cgtctgcggc tacgtgggcc
     7021 gctccaactc gacctccgga aagaacgcca ccaacagcgc cgctgtcctg gaaaacggca
     7081 ggatcgtgtt caggcaagtg aagatgctgt tgcccacgta tgacgttttc gacgaagcgc
     7141 gctatttcgt gcccgccgaa catcagttgc tcctcgagct gcgaggccga aaaatcgcgc
     7201 tcacgatttg cgaggacgcc tggaacgaca agcaattctg ggagcggcgc ctgtatcgcc
     7261 gggaccccgt cgaagagctc gtcgagcagg gggcgggggt gctgctcagc atcaacgcat
     7321 ccccgtacca catgaacaag cgcgagttgc gccgtgagat cttcgcggca accgcccggc
     7381 ggcacggact acccctcgtg tacgtgaatc aggtcggggg caacgatcag ctcgttttcg
     7441 acggcagcag cttcgccatg gacaaggacg cccgggtcat cgcctcggcc gcgtcgttcg
     7501 cggaggatct cgtgttcgtg gacaccatgt ccggcgaagg cgatcttcac gaaaacctcg
     7561 cggatgagca ggaggctgtt tacgaagcgc ttgtgctggg cacgcgggac tacattcgca
     7621 agtgcggttt ccgcaaggtt ttgctcgggc tgagcggtgg cattgattct tcgctgaccg
     7681 cagtgatcgc cgccgacgcg gttggtccgg agaacgtcat tggcgtggcg atgcccggcc
     7741 cctattcgtc ggagcacagc ctgcgggacg cacgggcctt agccgaaaga ctcggaattc
     7801 ggtgcgacac gacatccatc accggggtct atgacaagtt ccttgacgtt ctcgacccgt
     7861 tgttccaggg tgcgccgcga gacgtgaccg aggagaacct ccaggcccgg ctgcggggag
     7921 tcacgctcat ggctctctcg aacaagctgg gtgcgttggt gctgaccacc ggcaacaaga
     7981 gtgaactggc ggtcgggtat tgcacgctgt atggcgacat gatcggcgga cttgcggtca
     8041 tcagcgacgt gcccaaaacg cttgtttacg agttatcgcg agtggctaac tgccgccatc
     8101 cctgcgcgat tccggagagc gtcttcacca agccgccgtc cgcggaactc cggccgaacc
     8161 aaaaagactc cgattcgctt ccggagtacg cggtcctcga cgtcattctc cgcgactacg
     8221 tagaggagta caagacgccg gcttggatct ccgaaaaccg gggctttcct ctcgagctgg
     8281 tgcgtgacgt ggtcaacaag gtggaccgga acgagtacaa acggcaacag gccgctcccg
     8341 ggctgaaggt cacctccaag gcgttcggaa tcgggcgccg cttcccaatc gcccaaaggt
     8401 tcctcgaatg agacttgttc tgatcactct gcccgtagcc ctggccgccc tgctctcgtc
     8461 ccagcccgcg cctcgcgaac aggtgggtcc gctccccgat ggaggcttcc tgctcaacag
     8521 cggatggcgc ctctcgccgg cgggcaaaca ggttgcgctt gacacgttcc cgatgtcgat
     8581 ggcgctcact ccggacggga agcatctgtt ggtgctgaac ggcggctaca aaccgccatc
     8641 catcagcgtg atcgataccg cgtcggcagc agaaactgga cggacaccgg ttccggacgc
     8701 gtggctcggg cttacattta cgccgaaggg ggatctggtt tatattggcg gcggctccca
     8761 agcggccgtg ctcgaattct cgttcgtgga gggcaggctt accccgacac gaacgttcgc
     8821 ggtggttgcc gaagacaagc ggacccacca ggacttcatc ggcgacgtgg ccctgagtcc
     8881 ggacggacgc ctgctctacg ccgcggattt gtatcgagat tcagtcgtcg tcatcaaccc
     8941 tcagtccggc atcgtcatcg aacggatcaa aacgggccgc cggccctatc ggattttgtt
     9001 ccacccagac ggcaagtcct tctttgtctc gagctggacc gacggctccg tctaccatca
     9061 cgaatcggaa aagggaaccc tgctggggcg cgtccggatc gcatcgcacc caaccgacat
     9121 ggtctggcgg ccaggcaggc cggaggcgtc cgaacaggac gaggccgcgc cgccgtacgt
     9181 cgctcgggtg ttcgtagcgg ccgccaacac aaacagcgtg tacgtcatcg gcgtttcgga
     9241 agggaaggac cttcaggtgg ttgagtcaat caatgtctcc ctgacgccac gccaacccgc
     9301 cggaatgacg ccgagcgcct tggcgctcag caaggacgga aaacgtctct tcgtggcatg
     9361 ttcggatgcg aatgccgtcg cggtggttga tgtctcggaa ccccgcagca gggtgttggg
     9421 attcgtccct accggctggt acccaaccac ggttcgcgcg ctcgcggatg gccgactcgt
     9481 catcctgaac ggcaaggggc ttcgctcgta cccgaatcct cggggaccga acccgctggc
     9541 aagacccgag gctgcacacc tcggtccggt tagacccgag caggtccagt acgtcggtca
     9601 cctgcaaacc gggaccgcct cgtttgtcga ccccttcgat gacgagcgac tcgaggcaca
     9661 cacgcggacc gttctttcca attcccctta tcgcgactcg aagctcgatg acgccggcac
     9721 tggaaaggac aaccctatcc ccgcatttcc cggcggaacc acgccgatcg agcatgtgat
     9781 ctacatcgtc aaagagaacc ggacgtacga ccaggtgctc ggcgatctga agcagggcaa
     9841 cggagacccc tcactggttt tgttcggcga ggacgtcacg ccaaatcatc acaagcttgc
     9901 gcgtgagttc gtgctgctcg acaacttcta tgtgaattcg gacgtcagcg ccgacggcca
     9961 caactggacc accgccgcca tcgcccccga ttacgtgcag aagctctggc ccaacagtta
    10021 cgccaaacgc cgccggcact atgactacga agggcaggag gccgctgccg cgccgcccgc
    10081 cggttacctt tggaccaacg cggccggcgc ggagattccc atgcggaatt acggctactt
    10141 cgtctccaac cggccgaagc ccggcgcgga cggaatccag gtggacgcgg tgcgcgaccc
    10201 ggttctcagg cgcgtcacca acctccacta tcgtgggttc gacctggact accccgacgt
    10261 ggaacgagcc aaagtctttc tccgagacct ggctgatttt gagaaggcgg gcgcaatgcc
    10321 gcgcttgctg ctgctgcgtc tcggcaacga ccacacctcc ggcgcctcac ccggcaagct
    10381 tgccccaaaa tctgcggtcg ccgacaacga ctacgcgctt ggcatgatcg tcgaggccgt
    10441 gacgaagagt tcgttctggc cgaagacggc gatcttcgta ctgcaggacg acgcccaaaa
    10501 cggcgccgac catgtcgact cccaccgctc gccggcgttc gtaatttcgc ccttcgccaa
    10561 gcgtggcatc gtggacggca cgatgtacaa caccacctcg atgcttcgga cgatcgaact
    10621 gattctcggc ctccagccga tgactcattt cgacgccggt gcgcggccga tgtcggcgat
    10681 gttccagacc actcccaatc tcaaacccta ctccgctgag tccccgagga tcccgctaaa
    10741 cgagcgcaat ccggccgtgt cgcccgccgc cgcccgctcc gcgcgcctcg atttcagcga
    10801 ggaggatcgc atcgacgacg acgagttgaa cgagatcctc tgggtggctg ttcgcggttc
    10861 tgaaggagcg cctcctcccg cgccggtgcg tagctttttc tcccggtgag cccgatgcca
    10921 agaatctatc tgtgcttctt gtggcacatg caccagccgt tctacaagga cttgttctcg
    10981 caagagtaca ggctgccgtg gacccggctg catgccctga aagactactt cggcatggtg
    11041 gacgttctcc gtgatttccc ggaagtgaag caaaccttta acctcgttcc gtcgttattg
    11101 ctgcaagtcg aggaatacgc gtctggagaa gcggccgacc cgtttcttcg atgcgcgctg
    11161 aagccggctg aggctcttac cgacgccgag caggagttta tcctccgcta tttctttcag
    11221 gcaaaccctt cgcggttgat ctatcgcttc aaacgatacg gtgagttgta cgatgcgtgg
    11281 ctggcttcgg accgcgacgt gaagcgcgcc cggcggttct tcagctccca ggcgttccgt
    11341 gatctgcaag tgctttcgca gcttgcctgg tttgacgaga agtttctcga gcacgactcc
    11401 actgtcgcgg aactggtgcg gaaaggccgc gactacacgc tggacgacca ggagttgatg
    11461 ggccggaagc agaccgagat tctcggcaag gtacttccgg tctatcggga attcgcatcc
    11521 aagggtcaga ttgaaatctc gaccacgcct ttctaccacc caatcctgcc cctgctatgc
    11581 gactcggaca tcgccgggga agcccatcct cacgtgccgc ttccgtcccg tttccgctat
    11641 ccgcaggatg cacgcatgca actggagcgg tccaggcaat tcatccgcga ccgcttccgc
    11701 cgctatccgc gggggctgtg gccgtcggaa gggtccgtct ccgacgaagt ctttcaaatc
    11761 gcctccgagc ttggattcac ctgggccgcg accgacaacg gcgttctggc gcggaccctc
    11821 ggagacagcc ccggtgtcga aggcacgtat cgcccctacc tgtggcgcca gggcaagcgc
    11881 gagatccacg tgatttttcg cgatcacttc ctgagcgatc tggttggttt tgtctattcg
    11941 cgcatggggc cgggagaagc cgccgcggat ctccttcaac gcatccggga aaattgccgg
    12001 gggattctga acagtgggcg ggacgcgctg gttccagtca tcttggatgg cgagaacgcc
    12061 tgggaatact acgagcgcaa cggaaggccc ttccttcgcg agctttaccg ccggatcatg
    12121 gaggaccggc agatccgcgc ggtgacaatc cacgaggccc tcaagctgat cgaaccccag
    12181 cccatcgacc ggatttcccc cggctcgtgg atcaacgcga atttcgacgt gtggatcgga
    12241 gcggaagagg acaacaccgc gtgggaatac ctcttgcgcg cacggcagac gtacgatagg
    12301 gtcgcgtcgg gctcgcatgg agacgcacct tgggacggcg cccggctcaa gcttgccgaa
    12361 gaggagttgt tgatcgccga gggcagtgac tggtgctggt ggtacggtcc agagcacgat
    12421 tcagcgaacc ggcccgaatt cgaccagctc tatcgagacc acttggccaa cgcctatcgg
    12481 gcgctcgggc aggcgccgcc ggaggaactt tcgcggacga tcctcaaggt ggccgaagcg
    12541 gtataccacc acccgccaac gggcccggtc cgcgcggcga tcgacggaga agagacgtcc
    12601 ttttttgagt ggctcggcgc cggggtgtat cgggtggatt cgcggtcagg tgccatgcac
    12661 gggcaccggt tcctcatccg cgagcttcat tacgggagcg atgggccgaa tttctacatg
    12721 cgcctggact tggagccggt gtcgctcgaa ggcatggagg ctcggattaa catccggcca
    12781 tggactggcg actctccggc tagttgcatg cggttgcgtt ttgagcaggg caccgccgtc
    12841 actgtcgaac agcagttcgc cgctgggggg gaggcggagg tagcgtttca ccgggtactt
    12901 gaaattcgaa tccccctgat ggccgccagc ctcgtccagg gtcagcccgt tgcattgcag
    12961 gtatccatct gggaggacag cctgccgctc gacgccgttc cgcaccaggg ctggcttgag
    13021 tgctcgacag ccgagccgac cgactggcct gtctgagcgc accctctgcc ggcctcgatc
    13081 gcaccggcca tcactccacc agagggagaa tcctgataga ttctctggtg tcctatggtg
    13141 tcaaccaatc gcatttctca gtgcccgctc gatttctcgc gaatgctcct ttcgtgcgga
    13201 ctgacggtca ccgtggcact cgcggtactg gtcggttgcg gtccgctgac gaagaaagcc
    13261 gaggttggcg gcgaactcaa gcagtggcac aaagtcacat tgacgtttga cggaccgcgg
    13321 acggccgaag atgccacgcc gaatccgttc cgaaactacc ggctcaatgt gacgttcacg
    13381 cacggacaaa caggaacgtc cgtcaccgtt cccggctatt acgccgctga cggtcacgca
    13441 gcggaaagct cggcgacatc gggcggcaag tggcgggttc acttccggcc cgggcagcag
    13501 ggagagtgga gttacctggc gtcgttccga accgggcaag acgtcgccat cgatccggat
    13561 gccaacgcgg gacaaccggc ggcgttcgac ggcgccacgg gtactttcgt catcagtgca
    13621 acggataaga ccggcgtgga cctccgggcg aaggggttgc tccagtacgc cggcggccac
    13681 caccttcgct tcgcgggcaa cggagagtat tttctcaaag gtggcgccga cagcccggag
    13741 aactttctcg cgtatgccga gttcgacgga acatacgacg ccgacgccga ctcggggagt
    13801 tacaagaagg tcggtccgtt cattcaccgc tacgagccgc acatccgcga ctggcgtgat
    13861 ggagacccta cgtggaaagg cggcaagggt aaaggcatcg tcggcgcatt gaactatttg
    13921 gcggaacaag gcgtcaatag cgtctacttc ctcacctata acatcgacgg cggcgacggc
    13981 cgggacacgt ggatgtggac cggcccgaac gtccgcgacc ggttcgacgc cagccggctc
    14041 gaccaatggg agatcgtctt tgaccacatg gaccggaagg gcatcatgct ccacgtggtc
    14101 acacaggaaa cggaaaacga cagggccctc ggcggcgggc ccggtctgaa tcccatccga
    14161 aagctctact atcgcgagct gtgcgcgcgc tactcccatc atccggcgct tgtctggaat
    14221 ctgggggaag agaacaacac cagtgatcgg gatcgcaagg aggtcgcggc gtacatccgc
    14281 tcgctcgacg cgtggcggca tccgatcacg gttcacacgc acgcgggcca ggcgctgaag
    14341 ttctacaatg ggctgctcgg cgacggggac ttcgaggcga cgtcaatcca gggtgcattg
    14401 aaggactaca accgtgacgc aatcgtcctt cgcaaacgtt cggccgaagc cggacggaaa
    14461 tgggtgatct tcggcgacga gcagaacccg gcggatcgcg gtgttcttcc ggacttcgcc
    14521 gatcccgatc acaacgaacc gcgcatccag gctctctggg gaaacctgat gggtggcggc
    14581 agcggtgtgg agtggtactt cggattcgcg tttccccaca tggacatcaa ctgcgaggat
    14641 ttccgttccc gcgagcggat gtggagccag acccgctatg cgctcgagtt cttccggaag
    14701 cacctgccgt tttgggagat ggaaccggac aacagcctcg cgtccggcgc caaagacgct
    14761 cgggttctcg ccggaggaga cgccatttac gccgtccagt tgactgctgg cggtaacgca
    14821 gcgttgaaac tcggctccgg gacctacact gtccgctggt acgatccgcg tgcgggaggc
    14881 gaacttcaag ccggcaccgt tgataccatc cgggggccgg gcgtgaagtc catcggccgc
    14941 ccgccctcga atgccctccg ggactggatc gcactcgtaa aacgagccgc ggactgacgc
    15001 gaaatcgcgc gtagcgctgg taacataagc tctatgaccc gcaaagtcgc cgccggcgga
    15061 ggcctcgagt tggtcctccc gcagtgcgaa aagggaatcg gcgccatcgt tcggaaggca
    15121 gattcgccgg acctgatctc cggtgtccgc gttgtttcgc ataacctctg gccggatgac
    15181 cgcggttact tcatggagat tctccgcgtt ggccacgggc tcccggccgg tttccccccg
    15241 gcggaaacgc aggtttcgct cgccctcgga tatcccggga ctgtcaaggc gttccactat
    15301 catttgcacc agaccgattg ttgggcgccg gctgccggca tgttccaggt ggcgctcgtc
    15361 gatttacgga agggttcgcc gacattcgga gtgcgcaaca ctttgtatgc gggctcgctc
    15421 tggccggtgc agattctgat tccgcccggc gtgggccacg gctataagat catcggcgtc
    15481 gatccgggcg tcctggtcta cgtgaccgac cacttttaca atcccgacga tgagggcagg
    15541 atcccgtata acgaccctgc gattcactac gactgggaaa cacagcataa gtagatcttt
    15601 cggcgcttca gccggcaatt cgcggtcctg tttatgctcg gaagagacgt cccagcgtcc
    15661 ggcttgcccc tccgccgatc aacagaatcg cgcaaccgag aaggccgatc ccactcatca
    15721 cctcgccaag cctttcgggc gtgctcttcg ggagactcac caaaagctca tggcgcccgg
    15781 ctggcgcaat gaactgcgta aggcctgaac ggggagcagg ccctgccgga gagcttgcgg
    15841 actctccggc gacgcgcaca atccaccccg gatagtacag ttgtcgaatt tgaaccgtag
    15901 cgggttcgga taggtccgtg aacacctgga cttctctcgg accgagcctc ttgatacggc
    15961 cgctccccat ccctccggcc acaaccaccg gaggcgtttc cctcgacaga cgcagcaggc
    16021 cggccggctc cagagtctcc gggtccgacc atctggccca agcgcgcgtt aggtcgtccc
    16081 ctcgagagcg cagcagaagc tcgtactccg gcgtcatggc tgccttcttg ccgtaaagcc
    16141 acaagatgtg agcggatgga agtgcacagg aagcaacagc aatcagcaga agcgcgaact
    16201 caaccagccg tccccacggt tcgcgtgcca tcccttctcg tccgacggca agtgcgaaca
    16261 acaggcttga gaccgcggac aaaaccagaa ggaagcgcca tgggaactgg agaccttgaa
    16321 gcatgggtaa agcatcccag acccccgcgc tccacggtgt catcaaatag agcgacaccg
    16381 cagccgaaac caccgcaaaa acagccagcc gattacgccg gctccctcga ttcaggcaca
    16441 gcaaaaagct gaacaggcaa atcagtgtca gagcaatggc gatccaagtt gcataccact
    16501 tgaaaggatt gcccgctgca tcccatacga acagcgaaga atctgttacg agaaagttgt
    16561 ccttgtaata atagtgcggg ccggatgtaa accgctcaac ggatatattt tgttcataaa
    16621 gtaaagcagg cagcaaataa aaggatgata acagcgaggc gagggctgtc cccgcgatgg
    16681 ctcctgcgaa caccgtgcgg cggacatgag actgcataaa agtcaaaaca taacccgcga
    16741 caaagggaag caagagcgcc acgctcaaga tatgcgtaac aagtaacatc gtggcgccaa
    16801 ccgccatcaa gagaattcct gatcttctgg atatggcaat tcgttccaat gcgtacagaa
    16861 tcgttggcag ccacacaaat gcccagagtt cggctgctgc gcacctgacg tacagatcgg
    16921 cccaaagatg atagggagcc gccatataca gagcggcgcc ggcgaatgcc cctgtacgcg
    16981 aggtgagaag acgcagccac cgatacgtgg ccactccgga ggccatcatc gcaaccgcaa
    17041 gagagagccg aaactcaaag tgctccacgg atccaaacag gagttggcag agagggtaga
    17101 acagagaagc aaagtaatag aagaagggac catagacgaa gaagacgggg ctcccgaggc
    17161 ctgcattcgc gccctggagc cagcgaggat agaaatcacc agaccagaac tgttcccgga
    17221 actgaagata gcgtgcaagg tgattggcga aatccttcgc gttcggaaga gggccttgga
    17281 gcaagacggg gagagaaagc agcaaaccgg ctgctgcaat catcagcaga caacatagat
    17341 cagggcgtgc ccgccagaag ttcgccttgc gcattaggct acgataagcg taaacgaaac
    17401 ggttgctgtg tgcggcgtca caagccttcg tagacccgca ccgtcccctc caccgcgctc
    17461 tccacagtga agcgctggcg ggcgcgatcg atcgcgcaac gtcccatctc gcgccgaaga
    17521 gatgtatcgc acgccagccg ctcgattgcg tccgcgaatg cggccggatt cgatggatcc
    17581 accagccgtc ctgtcacgcc atcctcaacc acctcgggaa taccgccgct gcggcttgcc
    17641 acgaccggaa cgccgcaggc catcgcttcg gcaagaacgt tgccgaatgc ttccccaaca
    17701 gaaggtagta cgaagacgtc acaggcctgc aaaacagtct cgagcccgct ggagtggccg
    17761 agccagtgcg tccgttccgc cagcccaagc ttcagacttc gggcctgcaa ctcggtgagc
    17821 agtggtcccg cgcccgccac cagcagccgt acgggcacac ctcttcgcgc cagcagcgcg
    17881 caggcatcga gcagcacggc cggctgcttc cacggcaata gcgagaaggc gcccgcaatc
    17941 acaaagtctc ccggggcgat gggcagatcc cgtgccaatc gttcccgcgc tcccggatcg
    18001 ggcacgaaac gaccggtgtc cactccgttg taaacaacgt cgatcttcgc ctcggcgatg
    18061 ccaacgcgaa tcagacggtg tttaatgaac tcggagattg cggtgaatcg gctgataggc
    18121 tggcaggcaa gccgggctcg caggcggacg aggtgcgctc tccgggagtc cgctgcccac
    18181 tcgccggagt tggcttccgt gtacacgatg tggggaacgc cctgcaaacg agcgagccat
    18241 ggaatcgcgc tgaagtaggc aaagtaccgg acgtgcacga gcccaacccg gtattgctga
    18301 acgagggcgc caatctgcct gtagtaaccg agccggctgc ggtcgaaagc gcacgcccgc
    18361 accacggcgc ctgcgtcggt gaaccgctcc gtgatcggag cgggcagttc ctcggagaat
    18421 actaatatcg gctgcacgcc ggacgccctc atccgccggc agagccgcca atgaaactcg
    18481 gcctttgtgt cccaagtggc cggagagcaa tcgagaagat aggcaatgct ctccaggcgc
    18541 atcgtttcag attagcatct ggatcggaaa catccgtctc gccggaccgc tgctaggatt
    18601 ggaagatgcg gaggcgggtg cctatttgaa gcagctcttg agaggcgtcg tcagaattct
    18661 cttttattcc atggcggctc ttggtctggt ctggaccctc gccaccgtta ccccgatcga
    18721 ttaccactgg gcgacctggc tggccggccc atgggatgac ccggcggcag acgtgttgat
    18781 cgtcttgggt ggttccgtgt tggatgacgg cacgatcggc caaagttcgt actggcgtag
    18841 cgtgtacgcc gtccgtgcct ttcgcgaggg cgacgtccgg acagtggtca tttgcggcag
    18901 gagggcctcc gtggcgatgc gggagtttct cgaatcccag agcgtcccgc gctcggcgat
    18961 tcgaattgaa aaccggtcaa gcagcacgcg ggagaacgca ctgtttgcac gggaagtcct
    19021 ggccgacgaa ccggggcgga aggttcttct caccagtgac tatcacatgt tccgcgcccg
    19081 ccgcgtcttt caaaaggccg gcatcgacgt gttgccgcgg cccatccccg acgtccgcaa
    19141 gcaagtgggt tcatggcgcg cccgctggcc cgccttcctg gacctcgtgg aggagacgat
    19201 caaaatcggc tactacttcg ctcggggctg gatctgaaac ttccgctacc ggcgcttcca
    19261 cgccatgcgc cgccggcccg aaatcacgaa gtacatgccc gcgaacacgg cggcgttaat
    19321 catgcagaag tagtatggca ggcgaagaaa cttcggcctg agtctgacaa acgatcccag
    19381 aacggccagc agatagaaga cggcctgcag cccggcggcg aactggtaca gcggccttcc
    19441 cagcagcggc gccgtggcgg cggccattcc tatgagacag agcggcgccg ccaggcggcc
    19501 ggtcttgtgc gaaacgaaga aaaagagcgc cagccattgc gggggcatga gcagttcttt
    19561 cagctcacgc aattgctcga ggttgcccgc catgattcgt acccggcgtc cgaagccgcc
    19621 catctcgtgc gcttcctcta gtgcgacagc ggagggttcg tatgcgatcc ggtagccttt
    19681 ccgcagaatc cggatgggga tcacgtaatc gtcgttgatg gtgtccggcg ctggaaacgg
    19741 gtacagttct ttccgtatgg cgtagagcgc cccatgcgct ccgatgatcg agccgagcgt
    19801 ggcttcctgc cgcttcagaa aggtctcgta tttccaatag acgtcctccg gcggtccgat
    19861 gttgacgctg tcgtgtttca ccacccgata caggccgctc acggcgccca ctgccgggtc
    19921 ggcgaagttc gcgacaaggc gccggagtgc gcccggttcc agcatcgtcg ctgcgtcgga
    19981 aaacgcgacg atctcgccac ggagttgggg cacgctgtcg ttcagtacgg cgagtttacc
    20041 ccggttgagc ggataacgga gcagccgcac ccgattccca tcagcgtgac gcgccaccag
    20101 ctccggggtg ccgtcggttg atccgtcgga agcaatcacg atctccagcc tgtcggccgg
    20161 gtagtccagt gcgagcgcat tgcggatctt ggcctcaatg acgtgtgctt cgttgtacgc
    20221 cgccaccagc aacgacaccg ccggctcgca agccgacttt ttcaccggac ggggaaacag
    20281 ccgggcgagt aacgtcacca gcaggaagta accgccacac acataaaaca caacgccggc
    20341 gaacgtccag aaaaggatct ccaccggtat cattcaggcc gccgactcca ggattctctc
    20401 gaaagagctg gcgacacggt cccaagagaa ccgccccgcg acgtgctccc acgctgcctg
    20461 cgccatccgc gtccgctctc cttcgtcttc aagcagcgcg aggcactgtt cggcgaatgc
    20521 ttccggatcg tccgccagac ggatgttttc cggagacgac acatccaatc cctcggcgcc
    20581 gacggaggtg gataccaccg ccgttttcgc agccatcgct tcatagacct tcagccgcgt
    20641 tccgccgccg atgcgcaacg gcacaatgga caccgacgat ccccacaggt acggccggat
    20701 gtcggccaca gtacccgtca cgcggatcca cgggtctttc cggggcagtg tggcaatctc
    20761 atcccgtggt gtccgaccga caatggccag cgagcactcc ggcctcctgc ggcggatcaa
    20821 tggcaggacc tctcgaacga agtacaacac accctcaacg ttcggcgacc agtccatcga
    20881 tccgacgaag accagatcgg ataccgtggc gctcgactcc ggcgggcgaa agaaatcgac
    20941 gtcgacgccg gttggcgcca ccgaaacgcg agtcgctccg aaaagcgttc gcatcaggca
    21001 ggcgtcggtc tccgagaccg cgatgacgtg ccgggccgag cggcaggcct cgccttcgta
    21061 gcggagcatt ttctccgctt gcttccggaa ataccacctc cggataggat ctgccgtaag
    21121 agctgcgcgc cgccgccaga tgacggtctc cacgttgtgt tggaacagaa cgcaatcgct
    21181 caggtcgcgc acgttcacgg ccggagtgag gaagtcgcac acgatctggt cgaagcgctc
    21241 ggcggtgcgc agttcttcga tttgcctggc catccggctt gagcggtagc ggcttatggc
    21301 caccgggagg ctcgagaaca ggccggcggc tgcttgcgcc aaaaaggcgg tggagtcttt
    21361 gtgcgccggc ctgtgggaaa cggggtatgc gcgggtgctg tattcggcgc tgcggcgcgg
    21421 accttccgtc tcgcccgcct cggcaaaccc tacatagtgg atctcgtgcc gttggtgaag
    21481 cctccgcaac atttccagtg ttcgaatgtg tccgcctttc gtcgtcggat gcaggaagtc
    21541 ggctttaatc cagagaatct tcatcgcttc gcgggagcct gcgccatcaa gccctcccat
    21601 cgtagttcgc gcctgccggc aaggaaaatc tcgccccaga tctggagatt cagaaggcgc
    21661 cagatccggt cggtcacatc ctcgatatcc cgccggtgac ggtcgagcaa ttcaccgagc
    21721 gcccgcgtgt ccaccagcga agcgaccaac ccatcctttg atgtaagcat cgcaagtagc
    21781 cgctcggcct ccggccgccg cagccactgg cgcagaggag tgggaaaccc catcttcttc
    21841 ctgtaaacga tatcgccggg gagcaggtct tcgaccgcct tcttcagaat gtacttcccc
    21901 gtcccgcgcc ggatcttcat cgcgtccgga acggccgcgg cgaactccac gaactggtgg
    21961 tccagaaacg gcacgcggct ttcaatcgac gtcgccatgc tcatctggtc ctgtttcatg
    22021 agaagttcga ccaggtaggt cgtctgatcc gcgtacagca tccgtgcaag cggagaatgg
    22081 ccatcccctt catcccagta accgaggaac gagtcgtacg caccggcagc ccggcccgcg
    22141 tccgaaaata gctcggcctg ttccgcctgc gggaacgccg agtagaaatt gtccaggtag
    22201 agcgactcga tgctctcgcc gcgcccgagg aacgaatgct ggagctttcg gcgctgggag
    22261 gccgaaagga accgcgagtc cgcaatcgag gaagccacga ggcgccgcaa ccgcgcgggc
    22321 aggagcctgt agaagcccat caagctcctg ttgagcagat agaaccggta gcgggaatac
    22381 ccagcgaaca tctcgtcgct gccctccccg gtcagcacca ccttgacctc ttccgaagcg
    22441 agccgggaca gaaaatgcag ggacacgctc gacggccagg tgatcggctc gtcctcgtgc
    22501 cagacaagct tcggcagggc gttgaagaag tcttccatcg agacaatgac gtcccggtgc
    22561 tcggtgccga tccggcgcgc cacctgccgt gcatagccga gctcgctgaa cgcctcttcg
    22621 cggtagccga ctgcataagt cttgacgcgg ccggacacca tgcgcgccat cagtgccgcg
    22681 atggcgctcg aatccactcc accgctgagg aacatgccga gcggcacgtc gctcatcagc
    22741 cgcattcgaa ccgtctcttc gagccggcgg cggcactccg cgatccattc gccgtcgtcc
    22801 cgcctcacgc tcgttccggt gcgcggtaca tcccagtagc ggtcgatcgc cggcccctcc
    22861 gcgttcagcc gcaaccggtg tcccggcatc agcttgcgga tgcccgcaaa cagcgtccgc
    22921 tcgccgctcg aatatccgaa tgccaggtgc tctgaaagaa gcgattcgtc aaactccgga
    22981 gaaatggctg gatgttccag caacgccttg atctcagagg cgaagacaaa cagacggccg
    23041 ttccagaaat agtagaaagg tttgatgcca agacggtcgc gcgcgcagaa cagcgtccgc
    23101 gtcgtgcggt cccagatcgc aaacgcgaac atcccccgga acctgtcgac gcaccccggc
    23161 ccccactctt cgtatgcgtg gaggatcgtc tccgtgtcgg agcggcttct gtacatgtgt
    23221 ccagagcgtt cgagttcctt gcggatgtcc gcgtggttga aaatctcgcc gttgtagacg
    23281 atctgcaagt tcccggattc attcgtcatc ggctgggcgc cggtggccag atcgacgatg
    23341 ctcagccgcc ggtgtcccag cgaggcgaac tcgtcgctga agtagccgtc tccgtccgga
    23401 cctcggtggt gtatcgaatc ggtcatccgc cggagcacgc tgtccggccc cgcgggcggc
    23461 gaaaatgtca cgtagccggc gatgccgcac atggcgtcag gcgcgtgccg tcgcctttcc
    23521 ggccggcgag ggaacggccg tggaacgccg ggagtgtgcc ggaaggacgt tcggcacgcc
    23581 ccgagccgcc agttctcgct ccaccgcccg ggcgaacgcc gtgctgaggc cggcgagcgt
    23641 cgggaaaaac atgtgatacg ccacgctgga gaacagggct gtcgcggcaa aaccgatcag
    23701 cgagagcaga atggcgtatg acatcgccgc gatatcagcc gtttccggac ggttgcgcgt
    23761 cgacttgtag atgcggttcg ccgaccggac gcaaaagaat agagcggcgg cgtacagaaa
    23821 caaccccggc aacccgtttt cgcttgagag ctgcgtgtac atacagtggg tctcccgcca
    23881 catcgcgcgc tctccttctt ctccagcttt tttcgcctgc acggactgaa actgccccgg
    23941 cccgacgccg aagagcggat tccggatggt taattcaaca ctttccttga acagggaaag
    24001 tcgttgttgc gacgactcga tggcgcgcat ctggtatgag tccagaaaac cctgctccgt
    24061 cacctccggt ttcaacgtaa ctgcaagccg ggccgttact gcctcgggcg tggtcacgag
    24121 gaccgtgatc gcgatgacca tgcacacgag cataagcttg aatctgccgg ccgccgaagc
    24181 gtggacaaag atcaccgcgc acattgccgc gaacgccacc aggccgcccc tcgaacctgt
    24241 ctgcaggaag acatacagca ccggcgcggc acagagagcg gcgagcaggc ggcggaatgg
    24301 cacccggctc ttttcgatca gcaacaagta ccaaaacgga agacccagga gcagaatctg
    24361 cgcgagatcg ttggggttct cgagcatgcc taccggagct gccaggcggc cggccaccaa
    24421 cgtgccaaag tacagaacga tcaacgcggc ggccagaacc gccaccgcga gtgtgttcat
    24481 cacctgccgg cactgccgaa gagtctgcgc cagcccggcg atcataacgt acaccatgat
    24541 cgatttcagc cactggttcg ccaacagatt gacgctgcca ccgcgccagt aactgagtgg
    24601 cgtaatcagt accatccaga gcgtgaacgc cgtgagcaat tggccgggac gcgacttcag
    24661 cgcgcgcgct agggcgccgt taatcaacgc gagcagcacc gccagggttc ccagaatcag
    24721 tggcagacgg agctggtggc ctgcgaagtc cgccagccgg ccggtcgcga ggaagagaaa
    24781 tacaagcatg agcggcgccc cgagtcgcgc gatcgggctc ggctgcaccg cggcaacagc
    24841 ggtggcagtc cccgctgcgg ccggtgttgt gactacgcgg tttgtctgag gtcccggaac
    24901 catagttcca acatgagaag cgaccagagc cagtgatgat tgtcccggcg cccgctatcg
    24961 tgctcttcga gcagttgtcg tacaaactcc ggcgaaacga tcccgcggtt caaaaactgc
    25021 gaactcgtca cgtgctcccg gaggaaaccc cgcaacgatc ctcggaacca gggggccaac
    25081 ggaacgccga atcccatttt cctccggcgc aacacctcgg ggggaagccg gtcgcccaac
    25141 gcccggagca gaatgcgctt gcccgcgccg ttccggagct tccatccgtg aggaatcgcc
    25201 gatgccagtt ccgctagatt ctggtccagc agcgggcacc gcacctcgag ggagtttgcc
    25261 atcgacatgc gatctacctt caccagcatg tcgccggtca ggttcgccgt ggcttcgaag
    25321 tacatcgcct gtgtcagcac gtccgcgccc gacggcagaa ggcagtccgg gaacgtctgc
    25381 gtgagatacg ccaggtcagc cggcggcatc cattcgggtt tcagcaattg ccggcggaga
    25441 tagtacggcg agtagttgtt ctcgaaatag cgctccaacg ccgatggccg gctcgccatc
    25501 cgcagatagt ttttcccgta cgccgaatac ggcaatcggt ccgccgcgaa tgcaaggaat
    25561 cgccgcatca cgcggggtac ggaatcccac caccgcagct tctcgatcac acgaaagcta
    25621 tcgtagccgg cgaacagctc gtctccgccg tcacccgaca gagcgacctt tacgtgcttc
    25681 cgggcgaact cagaaacgat aaacgttgga atggcggatg aatccgcgaa gggttcgtcg
    25741 aaatcgcatg ccagcctcgt ggccaggtcc accgagtccg gccgtacgat gatctgatga
    25801 tgctccgtgc cgtaccgctt cgccaccagc gatgcaaact cgagctcgtt gtactccgcc
    25861 tcttcaaaac cgatcgagaa cgtcttgacc ggttcggacg actgaagcgc catcgacgcg
    25921 actaccaggc tcgagtcgat gccaccgctc aggaatgccc ccagtggaac gtctgcgacc
    25981 atccggatac gcaccgcttc gtcgaactgc tcgcgcagcc gtttcgccgc gtcctcttcc
    26041 gacatcctgg gcgcgacctt ctccgccggt ggcggcagcc tccagtagcg ctgctggcgg
    26101 caagatccgt ccgccccgta ctccagccac gttcccggca tcagctttcg tatcgcttgg
    26161 aacgggctat agggatccgg cacgtagttg aactggaaat acaggcggag tgcctctggg
    26221 tcaagctcga gcggcacccc ggctgcccgg agacacttca gctcgcttgc gaagtacagt
    26281 ccggacgcca ggactgcgta atacagcggt ttcttcccaa aacggtctcg tgcgagcaac
    26341 agcctccgcc gttttgcatc ccacagggcg aatgcgaaca tgcctcgtag gcggctcacc
    26401 gcgtccgccc ccgcgtcttc gtacaggtgg accagcgtct cggtgtc
//
LOCUS       DATIFQ010000260         5889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_659978, whole genome shotgun sequence.
ACCESSION   DATIFQ010000260 DATIFQ010000000
VERSION     DATIFQ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659978"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            478..1293
                     /locus_tag="VLE22_12255"
     CDS             478..1293
                     /locus_tag="VLE22_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSB15224.1"
                     /translation="MSALKKILLPVDYSDRSLMAAEHARFIAKQCGSQVTVLHVKESR
                     TDEDGGQAARRAAVTNLFLNELDATSVELPGDAAEVIVQYAREHGYDLIVMPTRGHSA
                     LRLFLLGSVTAKVLHDTETPVWTGVHSEQEALVPLTEVRHVVCAVDLGPQSDTVLRWA
                     GDFAARFGARLTLVHASAQLVPAFGVVHDPEFRAHLAELMSAQLADLLRKTGIGAEAR
                     LASGEPAKAVVEAARESKSDLLVIGRTPPGLMGRLRATAYAIIRQSPCPVISV"
     gene            1321..1773
                     /locus_tag="VLE22_12260"
     CDS             1321..1773
                     /locus_tag="VLE22_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15225.1"
                     /translation="MPFSLCLVLGLTAFALQSAAPQVNPGLPPDFSAKFDRLFLSQEP
                     LRYQDNRGFLASIQKAKLRPKERGLVRAKLKEFLSAKPKPRPYAPGSMYTGVASEIAF
                     LRLQALQVLADIGTKEDAAFIGHLHARADEHPLFDEESRKAIKRLETR"
     gene            complement(1868..4084)
                     /locus_tag="VLE22_12265"
     CDS             complement(1868..4084)
                     /locus_tag="VLE22_12265"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSB15226.1"
                     /translation="MKSHFLYRSIATLFLSLEAACAVSALPAQTEEDWVVVPDSRLLD
                     YANTVPNSPALVKSLGDAAEYVLRWKVPADAETWRIRRSHVELAFRKAIGLERLPERT
                     PLNARVVAKHDLGEYTIENVIFESRPGFPVPANLYRPKAPSAGKRAAILSPIGHYLGA
                     GKTAPTVQERCIGLVRMGFVVLSYDAIGQGERMVPGNIHHEAGYALMPLGETIAGWMV
                     WDSMRGIDYLMSLPDVDAGRIGVTGNSGGGLNTLFTAALDPRVRAAVVAGYTFEFNNW
                     LKYAGTHCTCTHLPGIFRSMEWFEIASLIAPRALLMIQGESDGIFPISGARRAGHAAE
                     AVYAVAGVPERARFVSLPGHPHDYSRPFREPMYGWMARYLLGRESTDPIPEGNVASFP
                     ERDPRLLCDPEGSILPRSPTVVDLARKQALSAIEKLPLEISSQAREWVRELSEPPERL
                     PHYLAPHQSRPTADAEGRYLKVSFASEDGQYIPGVLWLPEKRATPARTVIFADDRGKK
                     AVAESGMVQPMLDEGVAVFAVDLRGRGETLGWVTPRWNTSFRLTANQVLFGRPLAGRR
                     AFDFIRAVDYLRSRTDTASDEIVAVGLGSDVPAVLLAAATDDRIRRVAAAGYFHSLVS
                     QMRARRLAKASDLPNGWNDPQISGGVNAGDFPVDFGAVIPSALKMADIPDLLTLIAPR
                     RVLFCQALDNKAPDAQELTSRFRRVTVSAGEDWIRYEPGRSLDGKLLGEWMRRTKR"
     gene            4252..>5889
                     /locus_tag="VLE22_12270"
     CDS             4252..>5889
                     /locus_tag="VLE22_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15227.1"
                     /translation="MLSAVVAFALAGAVCSLAPTVIPFTAYPGSETSPAFSPDGMRVA
                     FVWNGEKEENSDIYVKSVDSAAVVRLTSDRAEDQFPAWSPDGKWIGFRRSSDNQNAIY
                     VISPGGGAERKLADVRGLLTGMSWSPDGKWLAAGELGFDDWSGIVLIPFDKGERRKLI
                     SKAAPSYYASPAFSPDGRFLAFVTWTNKQAEDLHLLELNGDYSAKGEPRRLTHHGGFV
                     RGVAWTPDGRWLVYSHSVDVRSDAWLWQVPVFGGGESKRLELAGVRATWPAVAHKGNR
                     LVYRSGGTDFDIWKLEAGEPPRESIFAKRWDFHPRFSCDGTRLAFSSRRGGGRSAIWV
                     SNADGSRPVQITSASDRTQDFPCWSPDGRSIAFNAAGEGGAQDIYVVDVAGGEPRQLT
                     RHPSGDDQPSWSRDGRWIYFHSNRGGRGEVMRVPAAGGEAVQVTDNGGYRPLESNDGR
                     TLYYVRPPTRSAAVTLFARPVAGGPEWQVLDSVLAWGFWVAERGIYYFPPGGDDGSLP
                     LRFYEFASGESRPVAVVRGRFVQDLTVAPDGKTVLFTA"
BASE COUNT         1059 a         1827 c         1875 g         1128 t
ORIGIN      
        1 cagggaggcc ctatccttat ggacaggtcg ctcgcgacct gatgggccac gcacactgga
       61 tctgccagac cgcccaactg gccatggttc aaagccggtt atctgctgtt atcgctcggc
      121 actgccagcg gccgccatac ggaaatcgcg ggtgctccgc gctcaagctg tcagtcccgc
      181 ctcaccgaag gcgactagaa tggaagtggc cggcctgctg ccgccccgaa taatccgatc
      241 ttttcggcgt cacctggggg ctggattttt ctaggcgaac tgaaatcttt ggcaggtagc
      301 gtggagcccg ctccgttcgc cgatcacaac gcgatctggg tccacgttag cctgtagtac
      361 cgcctcaggc ggatcggccc tctcccgtgc ctcgccggac tcttcacctg tcccgcccga
      421 ccacggcgct cggatacact gagatcaccg attacgaacg gagagaattt gcagaccatg
      481 tccgctctca agaagattct cctgccggtc gattactccg accggtcttt gatggcagcc
      541 gaacacgcga gattcatcgc aaagcagtgc ggttctcagg tgacggttct tcacgtcaaa
      601 gaatcgcgga ccgatgagga cggcggacag gccgcccggc gggccgcggt tacgaatctc
      661 ttcctcaacg aactggacgc cacctcggtg gagctccccg gcgacgcggc ggaggtgatc
      721 gtgcagtacg cgcgtgagca cggttacgat ctgatcgtca tgccgacgcg cggacacagc
      781 gctctgaggc tgtttctgct gggtagtgtg acggccaaag tcctgcatga caccgagacc
      841 cccgtctgga ctggcgtgca cagcgagcag gaagccctgg taccgctcac cgaagtgcgc
      901 catgtggtgt gtgccgttga tctgggtccg caaagcgata ccgtcctgcg ttgggcgggg
      961 gactttgccg cgcgcttcgg ggccaggctt acgctcgttc acgccagcgc ccagctcgtg
     1021 cccgcgtttg gtgtagtgca tgacccggag tttcgagcgc atcttgcgga gttgatgtcc
     1081 gctcaactgg ccgatctgct acggaaaacc ggcatcggcg ctgaagccag gctcgcttcc
     1141 ggcgaacctg ccaaggcggt cgtggaggcc gcgcgggaaa gcaagtcgga tctgctcgtg
     1201 attgggcgga cgccgcccgg cctgatgggg agactccggg cgacggccta cgccatcatc
     1261 cgccagtcgc cctgcccggt catcagcgtc tgatcaggcg ctctgaacag agagatgaga
     1321 atgccgttca gtctctgcct cgtgcttggg cttaccgcat ttgctctgca atcggctgcg
     1381 cctcaggtca atcctggctt gcctccagac ttttctgcaa agtttgatcg actttttctg
     1441 tcgcaagagc ctcttcggta ccaagacaac aggggattcc tggcatcaat ccagaaggcc
     1501 aagctgaggc caaaggagcg cggacttgtc agagccaaac tcaaggaatt cctgtccgcc
     1561 aagcccaaac caagaccata tgcgccgggc tcaatgtaca caggcgtggc gtccgagatt
     1621 gcgttcttga ggttgcaggc ccttcaggtc ttggcggaca tcggaacgaa ggaggatgcc
     1681 gccttcattg gtcaccttca tgccagagca gacgaacatc cgctttttga tgaggaaagc
     1741 aggaaagcaa tcaagagatt ggaaaccaga taaaaatcgc ttagacgggg ctgccgggtg
     1801 cggcgccgtt ttcggtgtac ccgcggactt atcagggtcg tttgattcct cagacgcaga
     1861 ggccagctca tctctttgtc ctccgcatcc actcgcccag cagcttcccg tcgagtgacc
     1921 ggccgggttc gtaacggatc cagtcttctc ccgccgacac cgtgacccgc cggaaccggg
     1981 aagtgagctc ctgcgcgtcg ggcgccttgt tgtcgagagc ctggcagaac aacaccctgc
     2041 gcggtgcgat gagcgtcaga agatcgggga tgtccgccat cttgagcgcg gatggaatga
     2101 ctgctccgaa atcgaccggg aagtctccgg cattcacgcc tccgctgatc tgcgggtcgt
     2161 tccatccgtt tggcagatcc gatgccttcg caagccggcg cgcccgcatc tgcgaaacca
     2221 ggctgtgaaa gtaccctgcc gccgccacac gccggatgcg gtcatccgtg gccgcggcaa
     2281 gaagtaccgc gggaacgtcg ctgcccaacc cgactgccac aatctcgtcc gaggccgtgt
     2341 ccgtgcggga tcgcagatag tccactgccc ttatgaagtc gaacgcccga cgtcccgcca
     2401 ggggtcgtcc gaacagaacc tggttcgccg tcaatcggaa gctcgtgttc caacgcggcg
     2461 ttacccatcc gagcgtctcg cctctgcccc gaaggtccac cgcgaacacg gcgacgccct
     2521 cgtcgagcat cggctgcacc atgccggatt ccgcgaccgc cttcttcccg cgatcgtccg
     2581 cgaagatgac ggtcctcgcg ggcgtcgccc gcttctcggg aagccagagc acacccggaa
     2641 tgtattggcc gtcctcgctg gcgaatgaaa ccttcaggta ccttccttcc gcatccgccg
     2701 ttggccggga ctgatgcggg gcgaggtaat gcgggagccg ctcgggtggc tcgctgagtt
     2761 cgcggaccca ttcccgcgcc tggctcgaaa tttctagcgg cagcttctcg atggcgctca
     2821 gggcctgctt tcgcgccagg tccaccaccg tcggcgaacg aggcagaatg gagccttccg
     2881 gatcgcagag tagccgcggg tcccgctccg gaaaactcgc cacgttgcct tccggaatcg
     2941 ggtccgtgct ctcccggccc agaagatatc gggccatcca accgtacatc ggttcgcgga
     3001 acgggcgcga ataatcgtgc gggtgtcccg gaagggaaac gaatcgcgca cgctccggaa
     3061 ctcccgcgac cgcgtacacc gcttcggcgg catgtcccgc ccgccgggcg ccactgatcg
     3121 ggaaaatgcc gtcgctttcc ccctgaatca tcagcagcgc gcgcggcgcg atgaggctcg
     3181 cgatctcaaa ccattccatg ctgcggaaga tgccaggcag gtgcgtgcac gtgcagtgcg
     3241 tgcccgcgta cttgagccag ttgttgaact cgaatgtgta cccggccacg acagccgcgc
     3301 ggacacgcgg gtcgagcgcg gcggtgaaca aggtgttcag gcccccgccc gagttgccgg
     3361 tcacgccgat ccggccggcg tccacgtcag gcaggctcat cagatagtcg atgccgcgca
     3421 tgctgtccca gaccatccac cccgcgatgg tctcgcccag cggcataagc gcgtagcccg
     3481 cctcgtggtg gatgttgccg ggaaccatgc gctcaccctg cccgattgcg tcgtagctca
     3541 ggacaacgaa tcccatccgc accaagccga tgcagcgctc ctgaaccgtt ggcgccgttt
     3601 tgcccgcgcc gaggtagtgg ccgatgggac tcaggatggc agcccgcttt cccgccgatg
     3661 gcgctttcgg gcgatagaga ttcgccggaa caggaaagcc gggccggctt tcgaagatga
     3721 cgttttcgat cgtgtattcg ccaaggtcat gcttagccac cacccgcgcg ttcagcggcg
     3781 tccgttcggg cagcctctcg agcccgatcg cctttcgaaa ggccagctcc acgtgtgacc
     3841 ggcgaatcct ccacgtctcg gcatcggcag ggactttcca tcgcagcacg tattcggcgg
     3901 cgtcgcccaa agacttaacc agagcgggcg agttcggcac ggtgtttgcg taatcgagca
     3961 gacgcgaatc cggcacaacc acccagtcct cctccgtttg cgcaggcagc gcgctcacgg
     4021 cgcacgccgc ctcgagggaa agaaacaatg tcgctatgga tcgatacaag aagtgtgatt
     4081 tcatctcgcg gtcccaccgc cttccgtcta cggtagcacc gtcaatcccg tcaatccgcg
     4141 gttttatgcg caactggtag acacaggcag tgacctgatg ttggtggaca gtctgcggtg
     4201 gtatagtgtc acgtcatcga cggaaatcaa gtcacaggca ggagagtcgt ggtgttgtct
     4261 gcggttgtcg ctttcgcact ggccggggcg gtgtgctccc ttgctccaac ggtgattccg
     4321 ttcaccgcgt atccggggag tgaaacgtcg ccggcgtttt ctcccgacgg gatgcgggtc
     4381 gcgtttgtgt ggaacgggga gaaagaggaa aactcggaca tctacgtgaa gagcgtggat
     4441 tccgcggcgg tggtacggct tacgagcgat cgggcggaag accagtttcc ggcgtggtcg
     4501 cctgacggca agtggatcgg gttcaggcga agttcggaca atcagaacgc gatctacgtc
     4561 atctcgccgg gtggcggggc tgagcgaaag ctggcggatg tccgtggcct gctgaccgga
     4621 atgtcgtggt cgcccgacgg aaagtggctg gccgcaggcg aactgggctt cgacgattgg
     4681 agcggcattg tcctgattcc tttcgacaag ggcgagcggc gcaagctcat ttcgaaagcg
     4741 gctccatcct actatgcatc gccggcgttc tcgcctgacg gacgtttcct ggcgttcgtc
     4801 acctggacga acaagcaggc cgaggacctc catctgctcg aattgaacgg agactattcg
     4861 gcgaagggcg aaccgcggcg gctgacgcat cacggagggt ttgtccgggg ggttgcatgg
     4921 acacccgacg ggcggtggct ggtttattcc cactcggtgg acgttcgttc ggacgcctgg
     4981 ttgtggcaag taccggtctt cggtggtggg gagtcgaagc gactggaact ggccggtgtg
     5041 cgggcgacgt ggcccgccgt cgcgcacaag gggaaccgtc tggtataccg cagcggcggg
     5101 accgacttcg acatctggaa gctcgaggcg ggcgaaccgc ctcgggaatc catcttcgcg
     5161 aaacgttggg acttccatcc acggttttcc tgtgacggca cgaggctcgc tttttcatca
     5221 cgccgtggag gtggccggag cgccatctgg gtttcgaacg ccgatggatc gaggcccgta
     5281 cagatcacga gcgcgtcgga ccggacacaa gattttccgt gctggtcccc ggacggccgc
     5341 tcgatcgcat tcaacgcagc gggcgaaggc ggtgctcagg atatatacgt cgtcgatgtg
     5401 gccgggggag agccgcgtca actgacccgc cacccgtccg gcgacgatca gcccagttgg
     5461 tcgcgggacg gccgatggat ctacttccat tcgaaccgcg ggggaagggg agaagtcatg
     5521 cgggttccgg ccgccggcgg ggaggccgtg caggtaacgg acaacggcgg gtaccggccg
     5581 ctcgaatcca acgatggccg gacgttgtat tacgtcaggc ccccgacacg ctcagcggcg
     5641 gtgacgttgt ttgccaggcc ggttgccggc gggccggagt ggcaagtgct ggattctgtc
     5701 ctggcgtggg gtttctgggt agcggagcgg ggaatctact acttcccccc gggcggcgac
     5761 gatggctcct tgcctctgcg gttctacgag tttgcctccg gagagagccg acctgtggcg
     5821 gtggttaggg gacggtttgt ccaggatctg acggttgccc ctgatgggaa gacggtgctt
     5881 ttcactgcc
//
LOCUS       DATIFQ010000261         2100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_660199, whole genome shotgun sequence.
ACCESSION   DATIFQ010000261 DATIFQ010000000
VERSION     DATIFQ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660199"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VLE22_12275"
     CDS             complement(<1..200)
                     /locus_tag="VLE22_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15228.1"
                     /translation="MQVILTVVDRDLSTSSWPGLSRPSTLIPFNKQGVDARDKRGHDE
                     SEFTAVGITCRLRRASPSTHRAP"
     gene            502..1791
                     /locus_tag="VLE22_12280"
     CDS             502..1791
                     /locus_tag="VLE22_12280"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HSB15229.1"
                     /translation="MVAGKDVCLRRLAKGNRAQEVRFNRFLGNSKVTVARLIEGWSEG
                     TAAVESRHVLAIQDTSEINFATTAARRRGLGEIGKGNGRGVLLHPMLAVDAEDGTCLG
                     LLSGQVWTRCGRRTVPHDRRDLSDKESRRWIATAHAAKPLLAGAATVTSIGDRESDIF
                     AAYTTVPEPRFHVIARSMHDRKLACGSGLYAASERMAVVDEDWIVLPARAQRQERIAH
                     LELRFGTISLARPQTKLLHHLPKSVSLTLVDVREPNPQAGTEPLHWRLLTTHAVESVQ
                     KAWRIVEWYKRRWLIEQFFRVLKTQGFKLEDSQITTADRLLKLVAIAAKAAVITIQLL
                     QARDGHGQQPAQLAFNANEVAALAALNHDLERKSRRLRNPHPPDSLAWAAWIIGRLGG
                     WDGYSSSKPPGPITIKHGLEYFHAVAIGWSLRDVCMP"
BASE COUNT          392 a          641 c          704 g          363 t
ORIGIN      
        1 ggcgctctat gcgttgaagg cgaagctcgc cgcaggcggc aagtaatacc gacggcggta
       61 aactctgact cgtcatgccc gcgcttgtcg cgggcatcca cgccttgctt gttgaacggt
      121 atcagcgtgg atggccggga caagcccggc catgacgatg tggaaaggtc acgatcgacg
      181 acggttagta taacctgcat tccgcttccg ctcatgctgt cgtcccgggc gagcgaacgc
      241 gaggcccggg acccatgacc accatgccat cgagaggaca gggattatgg gtccccgcct
      301 gcgcgctagg gcatgcacac atctggtgcg acggattgac tcgggtggga ttgaggggat
      361 tccagaatcg ggaatttgta tgatgtttat tgcggcactt cggctagggc gaggtgctgg
      421 catgctgcgg gagagcgact ggtcactggg tcggttcggg gatcgccgtc tcgatttagg
      481 ggggcggcgc tgctcgaacg tatggtcgcg ggcaaggatg tgtgcctgcg gcggctggcc
      541 aagggcaacc gggcgcagga ggtccggttc aaccgcttcc tcggcaacag caaggtgacg
      601 gtggcacgcc tgatcgaagg gtggagcgag ggcacggcgg cggtggaaag ccgccacgtg
      661 ctggcgatcc aggacaccag cgagatcaac ttcgccacca cagccgcgcg tcggcgcggg
      721 ctcggggaaa tcggcaaggg caacggccgt ggcgtgctgc tgcacccgat gctggcggtg
      781 gacgccgagg acggtacctg ccttggtctg ctgagcgggc aagtgtggac gcgctgcggt
      841 cgtcgcaccg tgccgcacga ccggcgcgac ctgtcggaca aggaatcgcg gcgctggatt
      901 gcgaccgctc atgcggccaa gccgctgctc gctggcgccg ccacggtgac gtcgatcggc
      961 gaccgcgaga gcgacatctt cgccgcctat accactgtgc ccgagccgcg cttccatgtg
     1021 attgcccgca gcatgcatga tcgcaagctc gcctgcggca gcggtttgta tgcggcgagc
     1081 gagcgcatgg cggtggtgga cgaggactgg atcgtgctgc cggcgcgcgc gcagcggcag
     1141 gagcgcatcg cgcatctcga actgcgcttt ggaaccatca gccttgcccg gccgcaaaca
     1201 aaactcttgc atcatctgcc caaaagcgtg tcgttgaccc tggtcgacgt gcgcgagcca
     1261 aatccgcagg ccggcaccga gccgttgcat tggcggcttt tgaccaccca tgcggttgag
     1321 agtgtgcaaa aggcctggcg catcgtcgaa tggtacaaac gacgctggct gatcgagcag
     1381 ttcttccgcg tgctcaagac ccaggggttc aagctcgaag acagccagat caccacggct
     1441 gaccgcctgc ttaagctcgt cgccatcgcc gccaaggcgg cggtcatcac catccagttg
     1501 ctgcaggcgc gcgatggtca cggccagcag ccggcgcagc ttgcgttcaa tgctaacgag
     1561 gttgctgccc ttgctgccct caatcacgat ctcgaaagaa agagcagaag gctgaggaat
     1621 ccgcatccac cagacagcct ggcctgggcc gcctggatca ttggccgcct cgggggctgg
     1681 gacggctatt cctcctccaa gccgccggga cccatcacca tcaaacatgg cctcgaatac
     1741 tttcatgccg ttgccatagg atggagcctg agagatgtgt gcatgcccta gcgccttcgc
     1801 ggggacgacg ggcggagagg acaagctcac gaccaacggg cgcaaaccac tcatcttaag
     1861 ggaagcgacc gactagccaa ggctggcgcg cgaggcgagc ggcgaattgc aacgtgcagc
     1921 tctgccggga ttgcgcgcgg ccacgcaccc gctatgctcg cgagcgggga acaggcagcc
     1981 tccccttcag atggatcgcc atccaagcgc tgttcgccgc aacccgcggg gagcgagcca
     2041 tggatggtac atttcttcag cttgcatttt atctggcagc aacgttcgcc gcggcattgg
//
LOCUS       DATIFQ010000262         3138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2576699, whole genome shotgun sequence.
ACCESSION   DATIFQ010000262 DATIFQ010000000
VERSION     DATIFQ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2576699"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(433..1182)
                     /gene="istB"
                     /locus_tag="VLE22_12285"
     CDS             complement(433..1182)
                     /gene="istB"
                     /locus_tag="VLE22_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HSB15230.1"
                     /translation="MNLAELQRSLRQLRLGGMAAVLETRLHQAQAEPMAPIDLLSCLV
                     SDELTRRGDRLLERRRKQAGFRDANRTLDTFDFSFNPKMNRSLVFELATCGFIAKRDD
                     ALFLGPGGTGKSHLAQAIGQAAIVQGYRVLYRETHVLLEELAEAVAEGTRKQYMDTLA
                     TVQLLIIDDFGMRKLPLTAAEDLLEIVMRRYERASTLLTSNRPVDDWGKLLGDTASVG
                     SMLDRLLHHGHVLKCGPRSWRTKTAGGEDRA"
     gene            complement(1179..2837)
                     /gene="istA"
                     /locus_tag="VLE22_12290"
     CDS             complement(1179..2837)
                     /gene="istA"
                     /locus_tag="VLE22_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HSB15231.1"
                     /translation="MSNVLSEEKQKQVIALGQLGWTLRRIEKETGVRRETAAGYLRAA
                     GVPVRPPGRWGRAPAAKPAKEVTTDFGGPSTPKPANEAGAPTDPEQTNPANESGEVTA
                     DSGPPTPSRSPSASACLPFHELIEAELAKGRNAVGIWQDLVDRSAFDGAYESVKRYVR
                     KLRGARVPEPCAVILTPAGEEAQVDYGSGPMVRDPHTGKYRRTRLFVMTLGYSRKSVR
                     LLVFESSAQTWARLHEQAFRRIGGSSRVVVLDNLAEGVLKPDIYDPSLNPLYRDVLAH
                     YGAIALPCRVGDPDRKGKVESGVGHAKKTPLKGQRFESLAEAQAYLDHWEQRWADTRI
                     HGTTKRQVSAMFAEEKPTLLPLPLEPFRYYQYGERTVHLDGCVEVEAAYYSAPPRWIG
                     RHVQVQWNLQWVRLIDPANGQLLREHLREQRGRHRIHAEDRPRRTPLGTLQVLARADK
                     AGEHIGTFAKAMHREQGQTAVRRIQGLLSFQKKYGAARVDQACAMALEMEISDFRFVR
                     RYLERNLQPALSLRQVDPLIRQLSLYRDLIDERTDSSHNQETKE"
BASE COUNT          546 a          977 c         1012 g          603 t
ORIGIN      
        1 aagcaagatg aggtctgctt tttcgagttg ggcgtcaatg ccttgatcaa cctcgctgcc
       61 agcactgatc ttccgatcgt gccagctatg aatgacctcg gcggtcaccc caaatccggc
      121 catacgcggt cacttcaaaa ccggccaacg gagacactca gggacagatc ctttttagcg
      181 gacagccgca cattcccgca acactttttc gcttcggcat cgccgcgctt ggcataaact
      241 gatcgagacc ggacggagga tagggcaacg cagggatgcg acccgagcgc cgattccggg
      301 gcctaaccct gcggaggcgg gcgccgctcg cctccggagt cgaagcgaag cggagactat
      361 agccagggga ggcaggaaag cgataaatcc tggggtcgca ggggcaaagc ccccggactg
      421 ccgacatgtt gatcatgccc ggtcctcacc gccggcggtt ttggtgcgcc agctacgcgg
      481 gccgcacttc agcacgtgac cgtgatgcag caggcgatcc agcatggagc cgaccgatgc
      541 ggtgtcgccg agcagcttgc cccagtcatc tacggggcgg tttgacgtca acaacgtgct
      601 ggcgcgttcg tagcggcgca tgacgatctc cagcaaatcc tcggccgcgg tcagcggcag
      661 tttccgcatt ccgaaatcgt cgatgatgag cagttgaacg gtcgcgagcg tgtccatgta
      721 ttgcttccgt gtgccctcgg cgacggcttc ggcgagttcc tcgagcagca cgtgtgtctc
      781 gcggtagagc acccgatagc cttgcacgat cgccgcctga ccgatcgcct gggccaggtg
      841 gctcttgccc gtcccgccgg gcccgagaaa tagcgcatca tctcgcttgg caatgaagcc
      901 acaggtcgcc agttcgaaaa cgagactgcg gttcattttt gggttgaagc tgaaatcgaa
      961 agtatcgagc gttctgttgg cgtcgcgaaa gccggcctgt ttgcgccggc gctccagcag
     1021 acggtcaccg cgtcgcgtca gttcatcgga cacgaggcac gagagcaaat cgatcggagc
     1081 catcggctcg gcctgcgcct ggtgtaagcg cgtttccagc acggcggcca tgccgccgag
     1141 gcgaagctga cgaagggagc gttgtagttc agcgaggttc attccttggt ctcctggttg
     1201 tgggatgagt cggttcgttc atcgatgagg tcacgataga gcgagagctg gcgaatcagc
     1261 ggatcgacct ggcgcaagct caaggcgggt tgtagattcc gctccaggta gcggcgcacg
     1321 aagcggaaat cggagatctc catttccagg gccatggcgc aggcctgatc cacccgcgcc
     1381 gcgccgtact tcttctggaa cgacagcagc ccctggatgc ggcgcacggc ggtctggccc
     1441 tgctcacgat gcatggcttt ggcaaatgtg ccgatgtgtt cgccggcctt gtcggcgcgg
     1501 gcgaggacct gcagcgttcc gagcggcgtg cgccgcgggc ggtcttcggc gtgaatccga
     1561 tgacggccgc gctgctcgcg aagatgctcg cgcaggagct gcccattggc tggatcgatc
     1621 agacggaccc attgcagatt ccactgcacc tggacgtgac ggccgatcca ccgcggcggg
     1681 gcgctatagt aggccgcttc cacctccacg cagccgtcca gatggacggt tctctcgccg
     1741 tactggtaat agcgaaacgg ctccagcggc agcggcagca gcgtgggctt ctcttcggcg
     1801 aacatcgcgg acacctgccg cttggtcgtg ccgtggatgc gggtgtcggc ccacctctgc
     1861 tcccaatggt ccaggtacgc ctgtgcttct gccaggctct cgaagcgctg gcccttcagc
     1921 ggcgtcttct tggcgtgtcc gacgccggat tcgactttgc cttttcgatc tggatcgccg
     1981 acgcggcaag gcagcgcgat tgcgccgtag tgagcgagca cgtcccggta cagcgggttc
     2041 agcgacggat cgtagatgtc cggcttgagc acgccttcgg cgaggttgtc gagcacgacc
     2101 acgcgtgaac tgccgccaat tcggcggaac gcctgctcgt gcaggcgcgc ccaggtttgc
     2161 gcgctggact cgaagactaa cagccggacc gacttccggc tgtagccgag cgtcatgacg
     2221 aagaggcggg tgcgccggta tttgccggtg tgcggatcgc ggaccatggg gccggagccg
     2281 taatcaactt gggcttcctc gccagcgggc gtcaggatga cggcgcaggg ctccggcacg
     2341 cgggcgccgc ggagtttgcg aacgtaccgt ttgacgctct cgtacgcgcc gtcgaaggcg
     2401 ctgcggtcca ccagatcctg ccagatcccc acggcgttgc ggcctttagc gagctcggcc
     2461 tcgatcaact cgtgaaacgg aaggcaggcg ctcgccgagg ggctgcgcga cggtgtgggc
     2521 ggtccggagt cggcggtcac ctcgccgctc tcgttggccg gatttgtctg ctcggggtcg
     2581 gtgggcgccc ccgcttcatt ggccggtttt ggcgtggaag gcccgccaaa gtcggtggtc
     2641 acctctttgg ccggttttgc agccggagcc cggccccaac ggccaggcgg gcgcaccgga
     2701 acaccggcgg ctctcaggta tccggcggca gtctcccgcc gaacgcctgt ctccttctcg
     2761 attcggcgca aggtccagcc gagctggccg agcgcaatca cctgcttctg cttctcttcg
     2821 ctcaagacat tgctcacgtt gacggagcct atcgctccgc cgaactcaat gtcctgagtg
     2881 cttcctccgt tggccggttt tcaggtgacc atcaatggcc ggatttgggt gaccgccgag
     2941 gtgtccgcag gaccccgatg aaatgtcgga attgctgtac ctgcgacgat cggccccaca
     3001 ggcggctgaa gacttccgca cgcatttaca cgcatttatc acactgcacg atgtgtaaag
     3061 gggtcttcga ccggactggt gccttcatca ataccatcaa agccgttggc agccagattc
     3121 agatcaagag atcgacaa
//
LOCUS       DATIFQ010000263         5866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1810617, whole genome shotgun sequence.
ACCESSION   DATIFQ010000263 DATIFQ010000000
VERSION     DATIFQ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810617"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3465
                     /locus_tag="VLE22_12295"
     CDS             <1..3465
                     /locus_tag="VLE22_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HSB15232.1"
                     /translation="FFKSDAPAGQNLYGCTAPNTWTLQSSGTSSGEANTASTIGGGTF
                     HWFAQKLGVDLQFKSFDVSSPISVSESSNRLTFSCPTCIVSGAGYSDPSWITALAGSK
                     IIGDISGSAGSFTGSLGGDVSGGQAATVVGRIQGRPVASTSPADGQSLVWSAAGNQWQ
                     PGTVSGSGGASSAADLNDFKVVKASGSLLTVNAGCSAVKPCVARFGNKSYAFTTSATV
                     AITFGSGQAFLYLDPSGTIVIGHSGLTISCAGCVSQNGITQFPTDSLPLFIWSATAGS
                     WDDTGADFRSFLSAKQVTAGTGVVVTEASGIAAVAVDTAVVPQYSTGAGAPSGSCTAG
                     QSFYLDTTNHNAYYCTNTNTWEQIDAAGGTGSPGGTTGAVQFHDGAGFGGDATNFAWD
                     NTNKHLTVLLRDKGGQVYNVKAYGAVGDYDTDDTAAIQAAIDAAATAPAAPAWANGGA
                     IVFFPTGTYRITAPLTLKGGVTLQGVSSRAWSSVIHTWDNITALIQDQGLATETVSAG
                     VRDLAIDGGNDASYGAYYGLHLRYCTNCVFDNVAITFTKSYAVNTVSGAHNKFNKVTA
                     TSCNQGMLFGQHKWQASKYVPTGTVVGPTTAHWVFWYEATTGGTTGVSEPTWPLVAGN
                     TVNDGSVVWTARTSPLGAFTNSYIEDTYAYGIGYNFTVSGTTVTIAGVDYTPYLLNGT
                     EQVFALLTGGTNNTLFHAIRTISNVQYSGGNTTFTIDSALDDRTGGASGFGSDVWIQG
                     GADNLLFVRGGFGTYIVGNDFAHLDDWSPASGAAFHSVWVGGGGFFLNEQAYITGVHT
                     DYDLTINDISGQAMWLSTFIDVGTRWGKTTFLAGGAGVPFSRLPSSNVSNGATTYCSD
                     CTVATPCASGGTGAMATRLNGAWSCGGGGGSGAPTDAQYVGLAVNGTLTNERVLTPGM
                     HMTLTDGGAGGNATMAFNPTDEDVFWMREEFYQATSSQWGNGAFGGATANSSYNANSQ
                     DANHWGVYRLEFAGSPANGWGASYRVGFNQLSIQDVMDSETWEYKCIVKPEGALTQVK
                     HRCGLLASPDADAQLNSVYVEHVGAASAGVWRLATCDASTCSTADSAVTASTAFTQFR
                     VWCSASSGGRCTEVSLSVNGGAAVTKTTNLPTSGTRTVPAVQVITTDTVNKYLGIDFW
                     SLKTAVTR"
     gene            3462..>5866
                     /locus_tag="VLE22_12300"
     CDS             3462..>5866
                     /locus_tag="VLE22_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15233.1"
                     /translation="MRKLLWLLALAFPMAGAVDNARVLGVTPTQAVIAYTAPSSAACT
                     LEVSESASYTPLVHDIDTALFASSNADSRAGNVNIGKSRTFVVGQRTVGTASDGKRYS
                     RALQADTVHYFRVTCGADTAAGTFRTANIPLGNSRSDALLADPSSAGEYLWLTPNWVD
                     RTGKYIDPQTGVLVRNVSLPRDATSSSDRLNQTFTSASGAAWTNSSNALADDASAATY
                     SGSASDWMVLLKNNLSPYSTHVVDYIQVQIKGSGAGATAADRTIQACLTSDGGTTCDG
                     DTLEIALDSTESIKTLGSSTPIYTWRPLSRVNLNTADITNNPNFGVRIRKKSASTDQI
                     SIQYAELDVYLSIRFAVSSGGDFAMFTENVTGNGFYHVGTQGAGGASSGIYAVHGETG
                     ETRWLGSLTFFSGGMKYCVEDNILWHATDPNIIYCQPASDGRLFKGTLTGNDAEAAQN
                     SDVSATWTQVGSDTISNLIRAFYNAHSAEYEVVYDPAKYACGVQGIQNNYAMLVCRRW
                     IQDSYGWLAVMDLTTEAIVALMPQFNCAAGRWCGQHAYDNPGHINLWYDTTHFLQGGN
                     TGLGKYQTKLAAAITATSQTSIMVESASLWNGAWGSAPADGEPVSQYNDHYLQPMKVG
                     DVFMIGSEYVRVTTKTSASSWVVSRGEFGSTPATYAVGAVLDAFCANTNTGNKYNTAT
                     HTVWKFLDDPYGKDTSGTTLWTNHYIGHLTARGHRIINSFNLLAIGTEGEDILNTPDP
                     VYNYTTGGFRFAGKYAPHEGNTYQTHATMHQTKASAWERNWALDVRPFVGGNLVSYTT
                     EGCT"
BASE COUNT         1200 a         1743 c         1825 g         1098 t
ORIGIN      
        1 ttcttcaagt ctgacgcccc agccgggcaa aacctgtacg gctgcactgc tccgaacacg
       61 tggacgctcc agtcctccgg tacatcaagc ggcgaagcga acactgcatc gactataggc
      121 ggaggaacgt tccactggtt cgcgcagaaa ctcggcgtgg acctgcagtt caagtcgttc
      181 gacgtttctt ctccgatctc ggtatcagaa tcctcgaaca ggctcacgtt ctcctgccca
      241 acgtgcatcg tatcaggcgc cggctactca gacccgtcgt ggatcaccgc tctcgccggt
      301 tcgaaaatca tcggcgacat cagcggaagc gctgggagct ttacgggatc gctcggcgga
      361 gacgtgtccg ggggacaagc ggcgacagtc gtgggccgga ttcaggggcg tccggtggca
      421 tccacttctc ccgcagacgg tcagagcctg gtctggagtg cggccggcaa tcaatggcaa
      481 ccggggacgg tcagtggaag cggtggcgca tcgagcgcag ccgacctcaa cgacttcaaa
      541 gtcgtgaaag cgagcggctc gcttctgacg gttaacgccg ggtgctcggc ggtgaagccc
      601 tgcgtcgcgc gattcgggaa caagagttac gcttttacca ccagcgccac tgtggcgatc
      661 accttcggca gcggacaggc tttcttgtat ctcgatccgt cggggacgat cgtgatcggc
      721 cacagcggtc tgacgatttc ctgcgcgggc tgcgtaagtc agaacggtat tacacagttt
      781 cccaccgatt cacttccgct attcatctgg agcgcaactg cgggatcgtg ggatgacacc
      841 ggcgccgact tccgctcatt cctttcggcg aagcaggtga cagccggcac gggagtggtc
      901 gtgactgagg cgagcgggat agcggcggtg gcggtggaca cggcggttgt tccgcagtac
      961 tccactggcg cgggcgctcc ttccggctcg tgcaccgccg gacagtcctt ctacctcgac
     1021 acgacgaatc acaacgccta ttactgcacc aatacaaaca cctgggaaca gattgacgct
     1081 gcgggcggaa ccgggagtcc tggcgggact accggggccg tgcagttcca tgacggcgcc
     1141 ggcttcggcg gtgacgcaac caacttcgcc tgggacaaca cgaacaagca tcttaccgtt
     1201 ctcctccgcg acaaaggggg acaggtttac aacgtaaagg catacggggc cgtgggcgac
     1261 tacgacactg acgacacggc ggcgatccag gccgctatcg acgccgctgc gacggctccc
     1321 gctgctcccg cctgggccaa tggtggagcg attgtgttct ttcctactgg gacctacagg
     1381 attaccgctc cattgaccct gaaaggcggc gttacgctgc aaggggtttc gagccgcgca
     1441 tggtcctcgg tcattcacac gtgggataac atcaccgcat tgattcagga ccagggcctc
     1501 gctaccgaaa cggttagtgc gggagtgagg gacctcgcca ttgacggtgg taatgacgct
     1561 agctacgggg cttactatgg cttgcatctg agatattgca cgaactgtgt gttcgacaac
     1621 gtagccatca cgttcacgaa gagttatgca gtcaacaccg tttcaggcgc tcataataag
     1681 ttcaacaagg taacggctac gtcctgcaac caggggatgc ttttcggcca gcacaaatgg
     1741 caagcgagca agtatgtacc aacgggcacc gtcgtaggac caacaacggc tcattgggtt
     1801 ttttggtacg aagccacaac gggtggaacg acaggcgtat ctgaaccgac ttggccgttg
     1861 gttgcaggta acacggtcaa cgatgggtcc gtggtctgga cggctcgcac atcacccttg
     1921 ggcgcgttca caaacagtta cattgaggac acctacgcct acggtatcgg atacaacttc
     1981 acggtgtctg ggaccactgt caccatcgcc ggggtggact acactccgta tcttctcaac
     2041 ggaacggaac aggtattcgc tctgcttacc gggggaacca acaacactct gttccacgcg
     2101 atcaggacga tttccaacgt ccagtactcc ggcggaaaca cgacattcac gattgattcc
     2161 gccctggatg accgtactgg tggtgcttct ggtttcgggt cggacgtgtg gattcaagga
     2221 ggggccgaca accttctgtt cgttcgtggg gggtttggca cctatatcgt cggcaacgac
     2281 ttcgcccatc tcgacgactg gagtcccgct tcgggggctg cgttccattc cgtgtgggtt
     2341 ggcggaggcg gattcttcct gaacgaacag gcatacatta ccggagtcca cacagattac
     2401 gacctcacca taaacgacat cagcgggcaa gctatgtggt tgtccacgtt cattgatgtt
     2461 ggaacccggt ggggaaaaac gacgttcctt gccggcgggg ctggagttcc tttttccagg
     2521 ttgccgtcga gcaatgtctc caacggcgca acgacgtact gttccgattg cacggtggcg
     2581 accccttgcg cctctggcgg aacgggcgcg atggcgaccc ggctgaacgg ggcgtggtcg
     2641 tgcggcgggg gcggcggctc gggcgctccc acggacgcgc agtatgtggg gctggcggtg
     2701 aatggcacgt tgacgaacga gcgagtgctg acgcccggga tgcacatgac gctcacggac
     2761 ggcggcgcag gcggcaacgc gacgatggcg ttcaatccca ccgacgaaga cgtgttctgg
     2821 atgcgggaag agttctatca ggccacttct tcgcagtggg gcaacggcgc ctttggcggg
     2881 gccactgcta attccagcta caatgccaac tctcaggatg cgaaccactg gggtgtatac
     2941 cggctggagt tcgccggttc acccgcaaat gggtggggtg cttcgtatcg ggtcggattc
     3001 aaccagttgt ccatccagga cgtgatggac tccgagacgt gggagtacaa gtgcatcgtc
     3061 aagcccgaag gggcgttgac ccaggtaaag caccgctgcg ggctacttgc ttcgccggat
     3121 gcggacgcgc agttgaactc ggtctatgtc gagcatgtcg gcgccgcgtc tgcgggagtg
     3181 tggaggctgg cgacgtgcga cgcgtcgacg tgctccacgg cagactcggc ggtgacggca
     3241 tccacagcat tcacgcagtt ccgggtgtgg tgctccgcgt ctagcggcgg acgatgcacc
     3301 gaggttagcc tgtcggtcaa cggcggggcg gctgtgacca agacaacgaa tcttcccaca
     3361 tccggcacgc gcaccgtacc ggcagttcag gtcatcacca ccgatacggt aaacaagtat
     3421 ctggggattg acttctggtc gctgaagact gcggtgacgc gatgaggaaa ctactctggc
     3481 ttctggccct ggcttttccc atggccgggg ccgtggacaa tgcccgcgtg ctgggcgtga
     3541 ctccgacgca ggcggtgatt gcctacaccg cgccgtccag tgcggcctgc accctcgaag
     3601 tgagcgagtc ggcctcgtac acgccgctcg tgcatgacat agacactgcc ctgttcgcct
     3661 cgtccaatgc ggactcccgt gcagggaacg tgaacatcgg caaaagccgg acgttcgtgg
     3721 tcgggcagcg cacggtggga acggccagcg acggcaaacg gtacagtcgc gctctgcaag
     3781 ccgatacggt ccactacttc cgggtaactt gtggggcgga cacggcggca ggcaccttcc
     3841 gcaccgccaa catcccgctt ggcaactccc gctccgacgc tctactggcc gatccatcga
     3901 gcgcgggcga gtatctgtgg ctcactccga attgggtgga tcgcacgggc aagtacatcg
     3961 acccgcagac cggggtgctt gtccgcaacg tgagcctccc gagggacgcc acctcgtcca
     4021 gcgacaggct gaaccagacc tttacttcgg cgtccggggc ggcgtggacg aattcctcga
     4081 acgctctggc cgacgatgcc agcgcagcga catacagcgg cagcgcatcg gattggatgg
     4141 tgctgctgaa aaacaacctc tcgccgtatt cgacacacgt cgttgattac atccaagtgc
     4201 agatcaaggg cagcggcgca ggggcgactg cggccgaccg gacgattcag gcgtgtctga
     4261 catcagacgg cgggacgacg tgtgacggtg acacgctgga gatcgcgctc gattcgactg
     4321 agagcatcaa gacgctggga agctcgactc cgatctacac ctggcgccca ctgagccggg
     4381 tgaacctcaa caccgctgat atcacgaaca acccgaactt cggcgtccgc atccgcaaga
     4441 agtcggcatc caccgaccaa atctcgattc agtacgcgga actggatgtc tacctgtcga
     4501 tccggttcgc cgtgtcgagc ggcggcgatt tcgcaatgtt caccgagaat gtcaccggca
     4561 acggtttcta ccatgtcgga acacaaggag cggggggagc atcctcgggg atttacgccg
     4621 ttcatgggga aaccggagag acccgctggc tcggttcatt gacgttcttc agcggaggca
     4681 tgaagtactg tgtggaggat aacatcctct ggcacgcgac cgacccgaac atcatctact
     4741 gccagcccgc cagcgacggc aggctgttca agggaaccct caccggcaac gacgcggagg
     4801 ccgcgcaaaa cagcgatgtt tcggcgacct ggacacaggt ggggtccgac accatttcga
     4861 acctcatccg agccttctac aacgctcact ccgccgagta cgaagttgtc tacgatccgg
     4921 cgaagtacgc atgtggggtg cagggaatcc agaacaacta cgccatgctc gtctgccgac
     4981 gttggattca ggacagctac ggatggctcg cggtaatgga cctcaccacc gaggcgattg
     5041 tggcgctgat gccgcagttc aactgcgcgg cgggccggtg gtgcgggcaa cacgcctacg
     5101 acaatcccgg tcacatcaac ctctggtacg acacgacgca cttccttcag ggtggcaaca
     5161 cgggactggg gaagtatcag accaagctcg cggcggcgat cacggccaca agccagacga
     5221 gcattatggt cgagagcgcg agcctctgga acggcgcgtg gggctcggct ccggctgatg
     5281 gggagccggt cagccagtac aacgatcact acctgcagcc catgaaggtg ggtgacgtgt
     5341 tcatgatcgg gtcggagtac gttcgcgtga caacgaagac atcagcgagt tcgtgggtgg
     5401 tatctcgggg ggagtttggc tcgactccgg cgacgtatgc cgtcggggcc gtgctggacg
     5461 cattctgcgc gaacacgaac accggcaata agtacaacac cgccacgcac acggtctgga
     5521 agttcctgga tgacccctac ggcaaggaca ccagcggcac gactctctgg acgaaccact
     5581 acatcgggca tctgaccgcg cgaggccacc ggatcatcaa cagcttcaac ctgctggcga
     5641 ttggaactga gggggaggat attctaaata cccccgaccc ggtgtacaac tacacgacag
     5701 gcggtttccg gttcgcgggc aagtacgcgc cgcatgaggg gaacacctac cagacgcacg
     5761 ccacgatgca ccagaccaag gcatccgcgt gggagcgcaa ttgggccttg gacgtgcgtc
     5821 ctttcgtcgg tggaaacctg gtttcttaca caaccgaagg ctgcac
//
LOCUS       DATIFQ010000264         4303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_278931, whole genome shotgun sequence.
ACCESSION   DATIFQ010000264 DATIFQ010000000
VERSION     DATIFQ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278931"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VLE22_12305"
     CDS             complement(<1..725)
                     /locus_tag="VLE22_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15234.1"
                     /translation="MDDGTKLTKRAFLQTIGAGAPAVRLMLGEGAAQQASAADAGKFL
                     PLDLSRFYTAAPRDLGPHDAARGLSDESAGDRLIRTLHGRQQLRGVPFELGPGDPAQK
                     AWIVLSRRAGPGLAPRAELAVGATAKYLCLAQFCNWDATERPAPGADPIERVGEVLAE
                     VALVYEDGSRHSVPIRRRFEVNALNYAWGHLCFAAVPHRAPVPRKLSDPLPNAFYWGD
                     LQIAVWDPNYPSDPSLDWQGTLWL"
     gene            complement(729..2621)
                     /locus_tag="VLE22_12310"
     CDS             complement(729..2621)
                     /locus_tag="VLE22_12310"
                     /inference="COORDINATES: protein motif:HMM:NF039643.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HSB15235.1"
                     /translation="MSGPDHRNRENRSASAAVILLLLLSGSAAAQEEEKPSKDTIVWA
                     LDDTDKVHPVTGNLLSQGKDRYTGKLTGPGEHQTSNSVWNAATRTVKLFAGRNEFVSF
                     QVVLEKEKNDLHKIFVNATDLLGANARISADSHIKLFKQLYVQLDGVWYPDALLLFDI
                     AGATPMELPDPASGVAGQRVQSVWIDVYVPHTLPPGSYTGQIIVLHRNTNKQAILNVE
                     LEVAPFTLPDEVGLEIDLMNYGFLNIERGWPDMVIDGPRHRAIEREFFRSAHEHRMTF
                     AVVPYNHDGSIPKGVKPELAEAGADVRVADWSSWDARFGPLLSGEAFADLPRARQPVG
                     HFFLPYNLMWPSDMRHWKKADYRTEHLRVSEQFRKHFAAKGWTKPQYQIYYNHKEHYQ
                     FFPWNLDEPTRPEDLDALRYLGEILKESFPANDPVRVLYRLDIGHFHCENVESCTHKR
                     ETGAKAVSVLNPFVGLWNVNSAHYYGNLAEGRKLKAQGKTVYFYTGTPHVAEPLVQSA
                     YWGWRGYRFEVDGICYWNATDWGDWDTDAPPADPYTNAGGRYKGFSMLFYPGAKFGYD
                     GPVPSIRLKTMRRGFQDFEYLRLIEKSGAKSRPALAAMADELLKSNGANYPKLRRALF
                     ELLSRR"
     gene            complement(2782..>4303)
                     /locus_tag="VLE22_12315"
     CDS             complement(2782..>4303)
                     /locus_tag="VLE22_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15236.1"
                     /translation="SYAANGLSWTTADKAGYPNNLMNGDKGDFAPRIGAAYKLNSKTV
                     VRGSYGMYYWTVPNSQMLSSQLGSVPLALEYTMMMDWWEVEPGKRVDWYDVFHMPLPG
                     IRVGDPNMIDITSLQSVPKPFGFTPFPRDMRNARVQQWNFTTEREIAPLTSLRLTYTG
                     NHGSNLMQTVQTNGTEPRYYYTLRTGQLPESSDQLRANPFWQELPYRQPVGYSNNNQL
                     QVNLERRSSKGLQYQWYYVFTRALSTSDADEGYASNPGMVAPDSVVLPDGVTNPVPLS
                     ERLRLVYSNVAGIPKHQVNWNFIYDLPFGRGKSLGGNASAALNHIIGGWQIASMGGFH
                     TGQWLTPDFNESDPGAWWAINYQMLRDPRLSKSEQQVINYQERRQLLYFAGYFDATGT
                     GLTNYRPALAPPGADGSNNVPVTLSSGETVDVPYVVYNSMPRNFIQGPSNWNADFSLF
                     KNFQIRENMRLRVTADAFNLFNHPNNKDPDLTTGLVDLGVSANAPRIIQFSARFDF"
BASE COUNT          774 a         1269 c         1404 g          856 t
ORIGIN      
        1 agccagagtg taccctgcca gtcgagcgaa ggatcgctgg ggtagttcgg gtcccaaacc
       61 gcgatctgca ggtcgcccca atagaaagcg ttcggaagcg ggtcgctcaa cttgcgtgga
      121 acgggcgcgc ggtgcggcac ggcggcgaag cagaggtgtc cccaggcgta gttgagagcg
      181 ttcacctcga agcggcggcg gatggggacg gaatgccggc tgccatcttc gtagaccagg
      241 gccacctcgg ccagcacctc gccgacgcgc tcgatcggat cagcgcccgg cgcgggccgt
      301 tcggtggcgt cccagttgca gaactgcgcg agacacagat actttgccgt tgcgccgacc
      361 gccagttcgg cgcgcggggc caagcccgga ccagcacggc ggctgagaac gatccaggct
      421 ttctgcgcgg ggtcgccggg gccgagctca aacgggacgc cgcgaagctg ttggcgtccg
      481 tgaagcgtgc ggatcagccg gtcgccggcc gattcatccg agagcccgcg ggctgcgtcg
      541 tggggaccga ggtcgcgcgg cgccgcagta tagaagcggc tgaggtcaag aggaaggaat
      601 ttcccggcgt ccgcggcgga ggcctgctgc gccgcgcctt caccgagcat aagccggacc
      661 gcgggtgcgc ccgcgccgat ggtctggagg aacgctcgct tcgtcagctt cgttccgtcg
      721 tccatggttc acctccgtga gagcagttca aagagagcgc ggcggagctt gggatagttc
      781 gcgccattgc ttttcagaag ttcgtccgcc atcgcggcga gcgcaggccg cgactttgcg
      841 ccgcttttct caatgagccg cagatactcg aagtcctgga aaccgcgccg catcgtcttc
      901 aggcgaatgg aggggaccgg cccatcgtag ccgaatttcg caccgggata gaaaagcatg
      961 gaaaagccct tgtaacggcc gccagcgtta gtgtacgggt cggctggcgg cgcgtcggtg
     1021 tcccagtcgc cccagtcggt ggcgttccag tagcagatgc cgtccacctc gaatctgtag
     1081 ccgcgccagc cccaataggc cgactgcacg aggggctcgg ccacgtgcgg cgtgccggtg
     1141 tagaagtaga ccgtcttgcc ctgcgccttg agcttgcgtc cctcggcgag gttgccgtag
     1201 tagtgcgccg agttgacatt ccagaggccg acgaagggat tcaggacgct gaccgctttc
     1261 gcgccggtct cgcgcttgtg agtgcaggac tcgacattct cgcagtgaaa gtggccgatg
     1321 tcgagccggt acaggacacg caccgggtcg ttcgccggga aggattcctt caggatttcg
     1381 ccgaggtagc ggagggcgtc gaggtcctcg ggccgcgtcg gctcgtcgag gttccacggg
     1441 aagaactggt agtgctcctt gtggttgtaa tagatctggt actgcggctt cgtccatcct
     1501 ttcgcggcga agtgcttgcg gaactgctcg ctgacgcgga ggtgctcggt gcgatagtcg
     1561 gccttcttcc agtggcgcat gtcggagggc cacatcaggt tgtaaggcag gaagaaatga
     1621 cccacgggct gcctcgcgcg cggaaggtcc gcgaaagcct cgccggagag gagcgggccg
     1681 aagcgcgcgt cccaggaact ccagtcagcc acacggacgt cagcgccggc ttcggcaagc
     1741 tctggcttga cgcctttcgg aatactcccg tcgtgattgt aggggaccac ggcgaacgtc
     1801 atgcggtgct cgtgagccga gcgaaagaat tcgcgctcga tcgcccggtg ccgaggcccg
     1861 tcgatgacca tatccggcca gccgcgctcg atgttgagga aaccgtagtt catcaggtcg
     1921 atttcgaggc cgacctcatc cggaagcgtg aacggagcga cctcgagctc gacgttcagg
     1981 atggcctgct tgtttgtatt gcgatggaga acgatgatct ggccggtgta gctccccggc
     2041 ggcagcgtgt gcggaacata gacgtcgatc cacacggact gaacgcgttg cccggcgacg
     2101 ccgctcgcgg gatcgggcaa ttccatgggc gtggcgcccg cgatatcgaa cagcagcagc
     2161 gcgtcgggat accagacgcc atcgagctga acgtagagct gcttgaacag cttgatgtgg
     2221 ctgtccgcgc tgatgcgcgc gttcgctccc agcaggtcgg tggcgttgac gaagatcttg
     2281 tggaggtcgt tcttctcttt ttcgagcacc acctgaaacg aaacgaactc gttccgcccc
     2341 gcgaacagct tcacggtccg cgtggcggcg ttccagacgg agttcgaggt ctggtgttcg
     2401 ccgggtcccg tgagtttccc ggtgtagcga tccttaccct gggacaacag attgcccgtg
     2461 acggggtgga ccttgtcggt gtcgtcgagg gcccacacga tggtgtcctt ggaaggcttc
     2521 tcttcctcct gagcggcggc cgagcctgac aagagcaaca gcagaatcac ggccgcactg
     2581 gcgctccggt tctcccgatt tctatgatct ggtccactca tttttcaggt ccccttgtct
     2641 ttggatgcaa gtttcatgtc gatcccccca gtctcctcta ttccaatagc tttcgcgaac
     2701 cgggaagtgc gcaaagataa ggggccgggt acgcagtcct gcgcccggcc ccttggggtc
     2761 accgctatcg cctgacgctc gtcagaaatc gaaccgtgcc gagaactgaa tgatccgcgg
     2821 cgcattcgcg gaaacgccca agtcaaccag cccagtcgta agatccgggt ctttgttatt
     2881 ggggtggttg aacaggttga acgcatccgc ggtaacacgg agccgcatgt tttcgcggat
     2941 ctggaagttc ttgaacaacg agaaatcggc attccagttc gatggcccct ggatgaagtt
     3001 cctcggcatg gagttgtaga ccacataggg gacgtccaca gtctcaccgg aggagagggt
     3061 caccggaacg ttgttgcttc catcagcgcc aggcggcgcc agggccggcc ggtagtttgt
     3121 caggccggtg ccggtggcat cgaagtaacc ggcaaagtac aacaactggc gccgttcctg
     3181 gtagttaatc acctgctgct cgctcttgct gagccgcggg tcgcgcagca tctggtaatt
     3241 aatagcccac caggcccccg ggtcgctctc gttgaagtcg ggtgtcagcc actggccggt
     3301 gtggaacccc cccatgctcg caatctgcca tccaccgatg atgtggttca gggctgcgga
     3361 ggcattacca ccaagcgatt tccctcggcc gaacggcagg tcatagatga agttccagtt
     3421 gacctggtgc ttgggaatgc cggcgacgtt gctgtagacg agtcttagcc gctcggacag
     3481 ggggacaggg ttggtcacgc cgtcaggcaa cacgaccgaa tcgggagcga ccatacccgg
     3541 gtttgacgcg tagccttcgt cggcgtccga ggtgctcagg gctcgcgtga acacgtagta
     3601 ccactggtat tgcagaccct tggacgatcg gcgctcgaga ttgacctgta gctgattgtt
     3661 gttggagtag cccaccggtt gacggtaagg caactcctgc cagaaaggat tcgcgcgcaa
     3721 ctggtcgctg ctctccggga gttggcccgt ccgcagcgtg tagtaatatc tgggctcagt
     3781 gccattggtc tgaaccgtct gcatcaggtt gctgccgtga tttcctgtgt aggtgaggcg
     3841 gaggctggtc agtggtgcga tctcacgctc tgtcgtgaaa ttccattgct gcacacgcgc
     3901 atttcgcatg tccctgggga acggcgtaaa cccgaacggc ttcggcacgg attgcaggct
     3961 ggtgatatcg atcatgttcg ggtcgccaac cctgatgccc ggcaggggca tatggaacac
     4021 gtcgtaccag tcgacccgtt tgccaggctc gacctcccac cagtccatca tcatcgtgta
     4081 ctcgagggcc agcgggacac ttccgagctg ggacgatagc atctgcgagt tcggtacggt
     4141 ccagtaatac attccgtacg acccgcgtac cacggtctta ctgttcagct tgtaagcagc
     4201 gccgatgcga ggggcgaaat cccccttgtc gccgttcatg aggttgttcg ggtatcctgc
     4261 tttgtccgcc gtggtccaac tgaggccgtt ggctgcatag gag
//
LOCUS       DATIFQ010000265         4022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1045461, whole genome shotgun sequence.
ACCESSION   DATIFQ010000265 DATIFQ010000000
VERSION     DATIFQ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045461"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VLE22_12320"
     CDS             complement(<1..1124)
                     /locus_tag="VLE22_12320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HSB15237.1"
                     /translation="MLKSSVQPEASAPPASAAYAYLIAVVAALSGLLFGFDIAVINGA
                     IVFLREQFLLSDFQTEVAAGALLLGCAGGAAVAGTLSDRFGRKKILILSAILFAVSSV
                     AAGLPRGMNEFIAARLLGGVAVGVASMLAPLYIAENSPPRIRGRLVAFNQMAIVSGIL
                     LAYLVNWLLSGIGPNAWRWMFAVAAIPAILFWIAMLFVPESPRWLIKQGRHEESMSIL
                     TRLVGPAEAKTEFEDIRQTVAEEENSLLALLQPGLRRPLVIAVALAILQQITGVNTVL
                     YYGALIFREQVGEQSRTAAIGVNVIVGATNFLATIIAIWIIDKVGRKPLLLTSAGGMG
                     VSLVLLGLTFQQTPPPATLILVVILCYVFCFAIGMGPGVWV"
     gene            complement(1099..2298)
                     /locus_tag="VLE22_12325"
     CDS             complement(1099..2298)
                     /locus_tag="VLE22_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB15238.1"
                     /translation="MRNDLSRRELLACAGAAALSPLAAFGRQAPAEPVSVAECASYGS
                     ELLPALERMFDQLGGLGRLVKGKTVAIKLNLTGIPSYRLGHTPAEDAHWTHPRVVAAT
                     VHLLGRAGARRIRLLESPWSTADPLEEYMLEASWEPRDILSAADGVEFENTNWLGNAK
                     RYSRFAVPGGGLLFPAYDLNHSYEDCDVFVSIAKMKEHATTGVTLSMKNCFGITPCTI
                     YGDGAGVDEPSEFPKGGRQPLHSGYRQPSKSALPEKDPTSSREGGYRVPRAVADLAAA
                     RPIHLAIIEGIHSMAGGEGPWIPRCAPVRPGVMVVGLNAVNTDAVATAVMGFDPMADH
                     GTAPFEKSDSTLRLAEELGVGTRDLRRIEVRGTSIRDALFDFRKQHPAKRIWTDSRLH
                     AQIFRAA"
     gene            complement(2319..3626)
                     /locus_tag="VLE22_12330"
     CDS             complement(2319..3626)
                     /locus_tag="VLE22_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15239.1"
                     /translation="MALNPEWLPMRWPGSWAEPAMLDLLKDTPFDCLVASARPVVDRA
                     RKAGLAVVRIGERAAGGGEAGPVIPVAPRSSVAHAPSSPIVAFTENVWPSIKLSAKED
                     RDVADTGPTGMPWVESNGWFVRMARALAPGRTIWIVADPPEEKSLRRAEFYALAVSDA
                     AAYGGRWVVSLDNELAAGVASGNREALAAWKKISAAAAFHKRQAAWADFRPFGVLGVL
                     SDFAGDNEFTATEAVNLINRRNLGFHILNRTRGAELSLEGLKAVLYLDRQAPEGALRG
                     KLLAFVRGGGLVVGAPSCMTLTEGAKPSDQTHVRFRLWTLGSGRIAIAKEEFADPYVL
                     ASDTHVLLSRRHDLLRVWNGSPAITYYAGSPDGRRAVVHILNYSAQPVEFMSVRVMQP
                     YRSARLWTAGGEPPAPLKVIPADGEAEFHLPPVGPYAAIELEA"
     gene            complement(3774..>4022)
                     /locus_tag="VLE22_12335"
     CDS             complement(3774..>4022)
                     /locus_tag="VLE22_12335"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HSB15240.1"
                     /translation="KKIFNVHFRNIKGKFLNFQETFPDDGDIDMIRAMRVYKEVGYDG
                     MIMPDHVPRIDGDAGGRQAFAFAFGYIQALIQLVNQES"
BASE COUNT          728 a         1395 c         1202 g          697 t
ORIGIN      
        1 acccaaactc ccggtcccat gccgatcgca aagcagaaca cgtagcacag aatcaccacg
       61 aggatcagtg tcgccggcgg aggcgtctgc tggaaagtca gccccagcag cacgagagac
      121 acgcccatgc cgccagccga cgtcaacagc agcggcttcc tccccacctt gtcgatgatc
      181 cagatcgcga tgatcgtcgc cagaaagttg gtcgctccga cgatcacgtt cacgccgatc
      241 gccgccgtac ggctctgctc gcccacctgc tcgcggaaga tcagcgcgcc gtagtagagc
      301 accgtgttca cgccggtgat ctgttgcagg atcgccagcg ccaccgcgat cacgagcggt
      361 cttcgcagcc cgggttgcag cagcgcgagc agcgaattct cctcttccgc cactgtctgc
      421 cggatgtctt cgaactcggt tttcgcctcc gccggtccca ccagccgcgt caggatagac
      481 atcgactcct cgtgccggcc ctgcttgatc agccagcggg ggctttccgg tacgaacagc
      541 attgcgatcc agaacagaat cgccggtatt gccgccaccg caaacatcca tcgccacgcg
      601 ttcggtccga ttccagagag cagccagttg acgagatatg ccagcaggat tccgctgacg
      661 atcgccatct ggttgaacgc caccagcctt ccgcgtatcc tcggcgggga gttctctgcg
      721 atgtacagcg gcgccagcat cgacgccacg cccaccgcga ccccgcccag cagccgcgcg
      781 gcaatgaact cgttcatccc ccggggaagc cccgcggcaa ccgacgacac cgcgaacaga
      841 atcgcagaca ggatcaggat cttcttccgc ccgaagcggt cgctcagcgt tccggcgacg
      901 gcagcaccgc ccgcgcatcc gagcagcaac gcgcccgcgg cgacctcggt ctggaaatcc
      961 gacagcaaga actgctcgcg caggaagacg atcgcgccat tgatcaccgc gatgtcgaaa
     1021 ccgaacagca accctgacag ggccgccacc acggcgatga gataggcgta cgcggcgctc
     1081 gccggcggcg cggatgcctc aggctgcacg gaagatttga gcatggagtc gggaatcagt
     1141 ccaaatgcgc tttgcggggt gttgcttgcg gaaatcgaac agtgcgtcgc ggatcgaagt
     1201 tcctcgcact tcgatccgtc tcaggtcgcg cgtacccacg cccagctctt cggcaaggcg
     1261 cagcgtgctg tcgctctttt caaacggagc cgtgccatgg tccgccatcg ggtcgaagcc
     1321 cattactgcg gtcgcaaccg cgtcggtgtt gacggcgttg agccccacga ccatgacgcc
     1381 tggacgcacc ggagcgcagc gcggaatcca cggcccctcg ccgcccgcca tcgagtggat
     1441 gccctcgatg atcgccaggt ggatcggccg ggctgccgcc agatcggcta ccgcgcgcgg
     1501 cacacgatag ccgccttcac gggatgaggt tgggtctttc tcgggcaagg cactcttcga
     1561 cggctgccgg tagccgctgt gaagaggctg ccttccgccc ttcgggaact cactcggttc
     1621 gtccacgccc gcaccgtcac catagatcgt gcatggcgtg atgccgaagc aattcttcat
     1681 cgaaagcgtc acgcccgtgg tggcgtgctc cttcatcttg gcgatcgaca cgaacacgtc
     1741 gcagtcctca taggagtggt tcaagtcgta cgcagggaac agcaggccgc ctccagggac
     1801 cgcaaaccgg gagtatctct tcgcattgcc gagccagttc gtgttctcga actcgacgcc
     1861 atcggccgcg ctcaggatgt cacggggctc ccaacttgcc tccagcatgt actcctcgag
     1921 cggatctgcc gtggaccagg ggctttcaag caggcggatt cgccgcgcgc cggctctccc
     1981 gagcaaatga acggttgccg caaccactcg cggatgggtc cagtgcgcgt cctccgccgg
     2041 ggtgtgcccg agacggtaac tcgggatgcc ggtgaggttc agcttgatgg cgacggtttt
     2101 gcccttcacc agccgcccga ggcctccgag ctggtcgaac atccgctcca gggccggcag
     2161 aagctccgag ccgtacgaag cgcactccgc cacggaaacc ggttccgccg gtgcctggcg
     2221 tccgaatgca gccagcggag agagggccgc cgcccccgcg cacgcgagca gttctcttcg
     2281 gctaagatcg ttgcgcattt tccgcaagcc ctccgaagct atgcctccaa ttctatagca
     2341 gcgtaggggc ctaccggcgg gaggtgaaac tcggcctccc catccgcagg gatcactttc
     2401 agcggtgcag ggggttcgcc gccggccgtc caaagtcgag ccgagcggta cggctgcatc
     2461 acccggactg acatgaactc caccggctgc gccgagtagt tgaggatgtg cactacggcg
     2521 cgccgcccat ccggcgatcc ggcatagtag gtgatcgccg ggctgccgtt ccaaacccgc
     2581 agcaggtcgt gccgccggct cagcaggacg tgcgtgtcgg acgccagcac gtacgggtcg
     2641 gcgaactcct ctttcgcgat tgcgattcgt ccggacccga gggtccacag ccggaaccgc
     2701 acgtgggtct gatccgaagg cttcgcgccc tcggtgagcg tcatgcagga aggcgctccg
     2761 actaccagcc caccgccgcg gacgaatgcg agtaacttcc cgcgcaaggc gccttccgga
     2821 gcctggcggt cgaggtacag taccgccttc aatccttcca gtgacaactc cgcgcctcgc
     2881 gtccggttca ggatgtgaaa gccgaggttc cgccggttga tcaggttcac tgcctccgtg
     2941 gccgtgaact cgttgtcgcc cgcgaaatcg gagagcacgc ccaacacgcc gaatggccgg
     3001 aagtccgccc acgccgcctg tctcttgtga aacgccgcgg cggcgctgat ctttttccac
     3061 gccgctagtg cttctctgtt gcccgaggcc actccggcgg caagttcgtt gtcgagcgac
     3121 accacccacc gcccgccata cgcggccgcg tccgagaccg cgagcgcata gaactccgcc
     3181 cgccgcaggg acttctcttc gggagggtcc gccacgatcc agatcgtcct tcccggcgcc
     3241 agcgcgcggg ccatacgcac gaaccagccg ttcgactcga cccacggcat cccggtcggg
     3301 cctgtgtcgg cgacgtcacg atcttctttg gcgctcagtt tgattgacgg ccagacgttc
     3361 tccgtgaacg cgacgatcgg cgaggacggc gcgtgcgcca cgctggaacg cggcgccact
     3421 ggaatcaccg ggccggcttc accgccgccg gctgctcgct caccgatccg gacgacggcc
     3481 agccccgcct tgcgtgcccg gtccacaacc ggccgggcgc tcgcgacgag gcagtcgaag
     3541 ggcgtgtcct tcaacagatc gagcatcgcg ggttccgccc acgagccggg ccagcgcatg
     3601 gggagccact caggattcag ggccattcgc tcaaccttcc ccaagaactg gggacagaca
     3661 gctgtgaaaa aaacctgggg acacgtctgc gcgccgttgc aggtgactca ttgcacaagg
     3721 ccaccccgaa gtgtccccgg ttttcttcac agcttccagt ccccggtttt tcttcagctt
     3781 tcctggttca ccaactgaat caacgcctgt atgtatccga acgcgaacgc gaaagcctgc
     3841 cgcccgcctg cgtcgccgtc gatacggggc acgtgatccg gcatgatcat gccgtcgtag
     3901 ccgacctctt tgtacacgcg catcgcccgg atcatgtcga tgtctccatc gtccggaaac
     3961 gtctcctgga agttgaggaa cttccccttg atgttgcgga agtgaacgtt gaagatcttc
     4021 tt
//
LOCUS       DATIFQ010000266         8618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1045465, whole genome shotgun sequence.
ACCESSION   DATIFQ010000266 DATIFQ010000000
VERSION     DATIFQ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045465"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VLE22_12340"
     CDS             complement(<1..1043)
                     /locus_tag="VLE22_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15241.1"
                     /translation="MSHSTFSRRAFLTRASLATFTVVTARRAPAMATTEESVQIHPHV
                     EIDMVRPELHGHFAEHLGTCIYGGLWVGHGSPIPNINGFRKQAVEYLRELGIPVLRWP
                     GGCFADDYHWRDGIGPVAKRPRRVNIHWGNYTEDNSFGTHEFIALCRLIGAEPYLAGN
                     LGSGTPQELRDWVEYCNYPSGSTLSGERAANGSPEPFRVRYWGVGNENWGCGGNMTGG
                     EYAAHYRRYSMYMREFGGTSPCLIACGPNGNDVAWSRGFMDGLGSYRNVSGFAMHFYS
                     GGRDTPTKFTVENMNEQLSSFARMEQAVVQQRALLDGYDPQRKIGLLVDEWGVWDRMI
                     PEEEKKYGRLWQQ"
     gene            1244..1561
                     /locus_tag="VLE22_12345"
     CDS             1244..1561
                     /locus_tag="VLE22_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15242.1"
                     /translation="MMKKIALLFVVMALSVAFAETYKVSLWQPALLGETELKAGNYKV
                     DVTNSKVVFTQGKNAVAESPVRVEKAEQTFNKSLVRYENENGKYRIREIRIGGTNMIV
                     VVN"
     gene            complement(1638..2399)
                     /locus_tag="VLE22_12350"
     CDS             complement(1638..2399)
                     /locus_tag="VLE22_12350"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HSB15243.1"
                     /translation="MRSPRFLIVNADDFGFTADVNRGIVEAHRNGILTATTLMANGAA
                     FDNALQLARENPSLDIGCHLVLIGGESVSDSRRLLPASVPELVRTVGLGRLSVHEELA
                     AQLRKLLAAGIRPTHLDTHKHTHLLPAVMAAVAGVSREFGIPWVRKPLPVPVLGSLLE
                     WKLRRGGSRTTDHFAGFSLTGRFGTAELVRCLKRLGSGSTEFMCHPGFCEDDLRRAHT
                     RLKESRVRELEALTSPETRRTIEEEQIHLVNYLDL"
     gene            complement(2396..3469)
                     /locus_tag="VLE22_12355"
     CDS             complement(2396..3469)
                     /locus_tag="VLE22_12355"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSB15244.1"
                     /translation="MPSAKKVKWAQLRVGVMAIAAMFVLAVLIFLLTGTKKLFARKAI
                     LHTYLDDSAALAVGAPVRLNGIVIGDVVKVELSGSKVPRRAVRIDLSVDSSKLSTIPV
                     DSEAAISAENVLGTKYINIRMGRSNDVVKDGGEIPAKDVSEFDEVVQSGYDVMVAARS
                     LLKRIDSMVSEIEKGKGTIGKLLVDEEFYNKLTGTVAETEKVAAAISSGQGTVGRLLY
                     DESLYNEVRGTVQRLDSLMTEVQEGQGTAGKFIKDPALYDEARKTVAELRRLTEDLNA
                     GKGTVGKLLKDEKLHQQIQSTIAKLEDTMDKINSGQGTLGQLMVNPSLYESLDGVSRE
                     MNGLLKDFRANPKKFLRIKLALF"
     gene            complement(3481..4326)
                     /locus_tag="VLE22_12360"
     CDS             complement(3481..4326)
                     /locus_tag="VLE22_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSB15245.1"
                     /translation="MSTSEQTMHPGADQSVLSFDRVSLSFEDTPALENVSFNITVGQS
                     CVILGAAGSGKTLLLKTALGLVKPDSGRVIVFGKDITGLKEAELFDMRARVGVLFQEG
                     GLFDSLTIEENVAYPLLNQRILTFPPDEVRPRVEEALRFVELEHTLDKFPSELSGGMR
                     RRVGIARAVVTEPALILYDSPTAGLDPITSHTIVSLIIKERDLRNATTVIVTHRYQDG
                     AWLADFRYNPKSGMLAPAANNGRRVDHLTKFMVLREGQKVFEGSRAELEASQDPYIAK
                     FVKQQ"
     gene            complement(4313..5101)
                     /locus_tag="VLE22_12365"
     CDS             complement(4313..5101)
                     /locus_tag="VLE22_12365"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB15246.1"
                     /translation="MVASKIRGTHGLLDLLKQPVLSVQEFLVLSGKAIRNVIRRPHYA
                     DDIFLQMDIIGVGSLPIVILTGFFTGAVMALQMSKALSQYGAVGKTGELVTISIVREL
                     GPVLTSLMVAGRNSSGMASELGSMKVTEQIDAMRALGTDPIQKLVTPRLIATCTVLPL
                     LTIIADFLGMFGGFLIALALLKMTPAQYWNSAWQAIVYSDIFQGLLKPFVYAFIVALV
                     GCHFGMQTTGGTQGVGRSTTRAVVVASVWIFVLDFFITRFFVNL"
     gene            5123..6082
                     /locus_tag="VLE22_12370"
     CDS             5123..6082
                     /locus_tag="VLE22_12370"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HSB15247.1"
                     /translation="MKGLTDIPGIRVGHASDFEALTGCTVVLCEAGAVAGADIRGSAA
                     GTEEFQVMNPMHVAETVHGVVLAGGSAFGLEAAAGVRRYLERRGVGFETRAARVPIVP
                     CAILYDLALGRPDVRPTREMGESTAAVAHDGPVEEGAVGAGTGASVGKLFGMSRAMKS
                     GLGSATVPLSGVYGGVLVSALAVVNAFGDVRNPETGQLVAGARRSSESLELADTAREM
                     KCGARGGFQGGNTTLVVVATNARLTKVQVTKLAQLAQNGVARAISPSHTMVDGDLVIA
                     LSIGAWDADVDALGVAANEATAQAVVRAARLAPGLGGLPGLRG"
     gene            complement(6099..7394)
                     /locus_tag="VLE22_12375"
     CDS             complement(6099..7394)
                     /locus_tag="VLE22_12375"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HSB15248.1"
                     /translation="MQERLNIAVFVDYDNIEIGVKSTLEREFDVSVALNALKERGDIV
                     AKFAYANWARQEGATRQMAANAVQMVQRIPSPRGDKNGADINLALDALEMAFTHNHVN
                     AFAIVSGDSDFIPLVNKLKEFGKTVFVVGGKAFTSTILQQNCHEFVSYESLLDEPQKP
                     QRQQQQQQHQGPHRQRQPRNRPAPLDPGQAVPLVERALQVVERRGLRPQLGLLKSTML
                     QLDSAFTEKAYGSGSFSDFVEKLKNAGYVNVAGEEGRYLIERKTSAKPEKPAPKPEEA
                     LPGLRDVLETHRLDIENGCLAGDLENWMQAEVPAFDPAAFGFQEFAEFLNYAQDKLLV
                     RVEPDEEKGLLVYLGAEFYPPAPPEAPTEPEVEVEVEEPQPYVKGQPSMVPEPEPKPH
                     RGRRRKDAASGAGAAPSKQRKPRQPRTTRARPRKTGPMV"
     gene            complement(7428..7829)
                     /locus_tag="VLE22_12380"
     CDS             complement(7428..7829)
                     /locus_tag="VLE22_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSB15249.1"
                     /translation="MEHPIRVLVAKPGLDGHDRGAKIIARTLRDAGMEVIYTGLRQTP
                     EMIASAALQEDVQVIGLSILSGAHNAIVPRVMELLHEKEMDDIIVIVGGIIPDEDAVQ
                     LKKQGVAAVFQPGASLEEIVTFIRSSVKQAA"
     gene            complement(7846..>8618)
                     /locus_tag="VLE22_12385"
     CDS             complement(7846..>8618)
                     /locus_tag="VLE22_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSB15250.1"
                     /translation="ARKMWAHLMRDRFGAKHERSLKLRFHAQTAGCSLTWQQPYNNVV
                     RTALQALAAVLGGAQSLHTNSLDEAYALPSEHAVTIALRTQQVIAHESGVAAVPDALG
                     GSYFLERLTIDVEQSAAEYIRRIDQMGGMIPAIERNFPQSEIAEASYRYQRAVETGDR
                     IIVGVNAFEQEDEKPIEILEIDRSAEVRQREKLAELRKRRDAARVEASLDALRRAAEG
                     RENTMPFLLDAVRCYATVGEICDTFRDVFGTWRETSVL"
BASE COUNT         1579 a         2597 c         2704 g         1738 t
ORIGIN      
        1 ttctgctgcc agagccgacc gtatttcttc tcctcttcgg ggatcatacg gtcccagacg
       61 ccccattcat cgaccagcag gccgatcttg cgttggggat cgtaaccatc gagaagtgcg
      121 cgctgctgaa cgacagcctg ctccatccgg gcgaatgaag aaagctgctc gttcatattc
      181 tcgacggtga acttcgttgg tgtatcccgg ccgcccgagt agaagtgcat cgcgaacccg
      241 gagacgttgc ggtagctgcc gaggccatcc ataaagccgc gcgaccacgc gacatcattg
      301 ccgttcggtc cgcaagcgat caggcagggt gacgtgccgc cgaactcgcg catgtacatc
      361 gagtagcggc ggtagtgagc ggcgtattca ccgccggtca tatttccgcc acatccccag
      421 ttctcgttgc cgactcccca atagcgcacg cggaacggct ctggcgagcc gtttgcggcg
      481 cgctcgccgg agagggtgct gccggagggg tagttgcagt actcaaccca gtcgcggagt
      541 tcctgtggcg taccgctgcc caggtttccg gcgaggtacg gctcagcgcc gatgaggcgg
      601 cagagcgcga tgaactcgtg ggtcccgaag ctgttgtcct cggtgtagtt cccccagtga
      661 atattcacgc gtcgcggccg cttcgccacc ggaccgatgc cgtcccgcca gtggtagtcg
      721 tcggcgaagc acccgcccgg ccaacgaagc accggaatcc cgagctcgcg aaggtattcg
      781 acggcttgtt tgcggaagcc gttgatgttc ggaattggag agccgtgtcc gacccaaagg
      841 ccgccgtata tgcaggtgcc gaggtgctct gcaaagtgcc cgtgcaactc gggacggacc
      901 atgtcgatct ccacatgcgg atggatctgg acggattcct ccgttgtggc catcgcgggc
      961 gcccggcggg ctgtgacgac ggtgaaggtc gcaagactag cccgggtcag aaacgcacgt
     1021 ctcgaaaaag tgctgtggct catgacggct cctcgcagca ggtttgtcac tcgtgcccac
     1081 ccgtttccac cgacgagtgg ccgggagatc catcttaact cgccaaagtg cgccaaagtt
     1141 cgccactcga acgacggcga cccttttctt cctggcacca catgatagtg gggttagtat
     1201 gattatggac attattcgta actaacaaaa ggagaaacgg acgatgatga agaagattgc
     1261 gctgctcttc gtggtgatgg ccttgtcggt ggcatttgcc gagacctaca aggtcagctt
     1321 gtggcagcct gcattgctgg gtgaaacgga actgaaggct ggcaactaca aggtcgacgt
     1381 cacgaactcg aaggtcgtgt tcacgcaggg caagaacgcg gtcgccgaat ccccggtgcg
     1441 agtcgaaaag gccgagcaga cgttcaataa gtccctggtc cgatacgaga acgagaacgg
     1501 aaaataccgg attcgggaaa tccggatcgg cggcacgaac atgatcgttg ttgtgaacta
     1561 atacctcttc ctcctcaaac acataggggg ggctccgggg cggcaacctc ggggctcccc
     1621 ttttgtttta cggggcctca caggtctagg tagttcacga ggtggatttg ctcttcctcg
     1681 atcgtacggc gggtttcggg ggaggtaagc gcctccagtt cacgcactcg gctctccttg
     1741 aggcgcgtgt gcgcccggcg gaggtcgtct tcgcagaagc ccggatggca catgaactcg
     1801 gtagaaccgc taccgagcct tttcaggcat cggacgagtt ccgccgttcc gaaacggccg
     1861 gtcaaagaga agccggcgaa gtgatcggtc gtgcgagagc cgccgcggcg gagtttccac
     1921 tcgagcaggc tgcccaagac gggaaccggg agcggttttc ggacccatgg aattccaaac
     1981 tccctggaaa cccccgcgac tgctgccatc acggcgggta gcaggtgcgt gtgcttatgg
     2041 gtgtccaggt gagtggggcg gatgccggcg gcgagtagct tgcggagctg cgcggcgagc
     2101 tcttcgtgaa cggacagacg gcccagcccg acggtgcgaa ctagctccgg cacgcttgcg
     2161 gggagcagcc ggcgcgagtc cgaaacggat tcgcctccga tcaacacgag atgacagccg
     2221 atatccagag atggattctc tcgggcaagc tggagggcgt tgtcaaaagc ggctccgttg
     2281 gccatgagcg tggtggcggt caggattccg ttgcggtggg cttccacgat gccccgattg
     2341 acgtcggccg tgaagccgaa gtcgtcggcg ttgactatta ggaaacgcgg agatctcaaa
     2401 acagcgccag cttgatgcgc aggaatttct tcgggttcgc gcgaaaatct ttgagcagac
     2461 cattcatctc ccggctgacg ccgtccaggg actcatacag gctgggattg accataagct
     2521 ggccaagagt accttggccg gagttgatct tgtccatggt gtcctcgagt ttagcgatgg
     2581 tgctctggat ctgctgatgg agcttctcgt ccttgagcag ctttccgacc gtacccttcc
     2641 ccgcgttcag atcttcggtg aggcgccgca gttcggcgac ggtcttccgg gcttcatcgt
     2701 aaagggcggg atctttgatg aacttgccgg cggtaccctg cccctcctgg acctcagtca
     2761 tgagcgagtc gagccgctgc accgtcccgc ggacttcgtt gtacaggctc tcgtcgtaga
     2821 gcaaccggcc gactgtgccc tgaccactgc tgatcgccgc ggccactttc tcggtctcgg
     2881 cgacggtgcc ggtcagcttg ttataaaact cttcgtccac gagcagcttg ccgatcgtgc
     2941 ccttcccctt ctcaatctcg ctgaccatgc tgtcgattcg tttcagcagc gaacgcgcgg
     3001 cgaccatcac gtcgtaaccg gactgaacga cctcgtcaaa ctcgctgacg tctttggccg
     3061 gaatctcgcc gccgtccttc accacatcgt tgctcctgcc catcctgata ttgatgtact
     3121 tcgttccgag aacgttttcg gcgctgatgg ccgcctcgga gtcgaccgga atcgttgaca
     3181 actttgagga atccaccgac aagtcgatgc ggacggcacg gcgcgggacc ttcgagccag
     3241 atagttcgac tttgacgaca tcgccaatga cgatgccgtt cagccggact ggggcgccga
     3301 ccgccaatgc ggccgaatcg tccaggtacg tgtgaagtat agccttacgg gcaaacagtt
     3361 tcttcgtgcc ggtaagcagg aagatcagca ccgccagcac gaacatggcg gcgatcgcca
     3421 tcacgccgac cctcagttgg gcccacttca ctttcttcgc cgaaggcatc gttttcctgt
     3481 ctactgttgc ttcacgaact tggctatgta gggatcctga gaagcctcga gttcggcacg
     3541 gcttccctca aacaccttct gtccttcgcg gagaaccatg aatttcgtca gatggtccac
     3601 gcggcgcccg ttgttcgcgg cgggcgccag catgccgctt ttcggattgt aacggaaatc
     3661 tgccagccag gcgccgtcct ggtaccggtg agtgacgatc accgtggtcg catttctcaa
     3721 atcgcgttcc ttaatgatga gggagacgat cgtgtgggac gtgatcggat caaggccggc
     3781 cgtcggagaa tcgtacagga ttagcgctgg ttcggtaacc acggcccgcg caattccgac
     3841 gcggcggcgc atgccacccg agagctcact gggaaactta tcgagcgtgt gctcgagttc
     3901 gacgaaccga agcgcctcct cgacgcgcgg ccgcacctcg tcgggaggga acgtcagaat
     3961 acgttgattg agaagaggat acgccacgtt ctcctcgatg gtgagcgaat cgaacaggcc
     4021 tccctcctga aagagcacgc ccacccgggc ccgcatgtca aaaagctcgg cttccttcag
     4081 gccggttata tccttgccga agacgatcac ccgtcccgag tccggcttga ccagcccgag
     4141 tgctgtcttc agtagcaagg tcttgccgct gccggcggcg ccaagaatga cacagctctg
     4201 tcccacggtg atgttaaaag agacgttttc aagcgcagga gtgtcctcaa atgacagcga
     4261 cactcgatcg aaggagagga cggattggtc ggcgccggga tgcatcgtct gttcagaggt
     4321 tgacaaaaaa cctcgtgatg aagaagtcaa gcacgaagat ccacaccgag gccaccacga
     4381 cggcgcgggt cgtcgaacgt cccacgccct gggtcccccc ggtcgtttgc atgccaaagt
     4441 ggcatcccac cagcgccacg atgaacgcgt acacgaacgg cttgagaagg ccctggaaaa
     4501 tgtcgctgta gacaatcgcc tgccaggcgc tgttccaata ctgcgcggga gtcatcttga
     4561 gaagggcgag ggcgatcaga aagccgccga acatgccgag gaagtcagcg atgattgtca
     4621 gtagcggcag gacagtacat gtggcaatga ggcgtggggt gacgagcttc tggatcgggt
     4681 ctgtgccgag cgcgcgcatg gcgtcgatct gctcggtcac tttcatggag ccgagttcgc
     4741 tggccatccc ggaagaattg cgtcccgcga ccatcagcga tgtgagtacg gggccaagct
     4801 cgcggacgat gctgattgtc acgagttcgc cggtctttcc gacagcgcca tactgggaga
     4861 gcgccttgga catctgcaac gccatgacag cgccggtgaa gaagccggtc aggatgacaa
     4921 tgggcagact ccccacgccg atgatgtcca tctggaggaa gatgtcgtcg gcgtaatggg
     4981 gccggcggat gacgttccgg atcgcctttc ccgacagtac caggaattcc tgtaccgaca
     5041 ggactggctg tttcagcagg tccagcaaac cgtgtgttcc ccgaattttc gatgctacca
     5101 tacaacctgc gagctgaacc ccgtgaaagg attgactgat atccccggaa ttcgtgtcgg
     5161 ccacgcgtcc gatttcgagg cgctgacggg ctgtacggtg gtcctttgcg aggctggcgc
     5221 ggtagccggt gctgacattc gtggctcggc agccggcacc gaagagttcc aggtgatgaa
     5281 tccgatgcac gtcgccgaga cagtgcatgg cgtcgtgctg gctggcggga gcgcgttcgg
     5341 cttggaggct gcggccggcg tgcggcgata cctggaacgg cgaggtgtcg gtttcgaaac
     5401 gagggcggct cgggtcccga ttgttccctg cgcgatcctc tatgacctag ccctgggccg
     5461 gccggatgtt cgtcccacac gcgagatggg tgagtcgacc gcggcggtgg cgcacgatgg
     5521 gccggtcgag gaaggcgcgg tgggcgcggg gactggcgcg tcggtcggca agctgttcgg
     5581 catgagccgg gcgatgaaat ccgggctggg gtccgccacc gtcccgctga gcggagttta
     5641 cggtggtgtg ctcgtctccg ctctcgcggt ggtgaacgcc ttcggagacg tgaggaaccc
     5701 ggaaaccggt cagctcgtgg cgggagcccg gcggtcgtcg gaaagtttgg agctggccga
     5761 taccgcccgc gagatgaaat gcggggcccg cggcgggttc caaggcggaa atacgacgtt
     5821 ggtcgtggtg gccacgaatg ccaggctcac caaagtgcag gtgacgaaac tcgcgcaact
     5881 cgcgcagaac ggcgtcgcgc gggcaatctc gccctctcac acaatggtgg acggcgacct
     5941 ggtgatcgcc ctctcgattg gcgcctggga tgccgacgtg gacgcgctgg gtgtggccgc
     6001 aaacgaggcg acagcgcagg cggttgtgcg tgccgcgagg ctggctccgg gcctgggcgg
     6061 cttgccgggg ctcagaggat agtcgagcgg acgccgcgtt acaccatggg cccagttttt
     6121 cgcggacgag cgcgcgtcgt gcgcggctga cgcggtttgc gctgctttga aggggccgcg
     6181 ccggcgccag aagccgcgtc cttccgccgc ctgccgcggt gcggcttggg ttccggttcc
     6241 ggaaccatgg acggctggcc cttgacgtat ggctgaggtt cctcgacctc cacctccacc
     6301 tcgggttcgg ttggcgcctc cggaggcgcc ggcggataga actcggcgcc gaggtacacc
     6361 agaagtcctt tctcctcgtc cggctccact cgaacgagca gcttgtcctg ggcgtagttc
     6421 aagaactcag caaactcctg gaagccgaat gctgccggat cgaaggccgg tacttcggct
     6481 tgcatccaat tctctagatc accagccaga catccgtttt cgatgtccag gcggtgcgtc
     6541 tcgagcacat cccggagtcc ggggagggcc tcctcaggct tcggcgccgg cttttcgggc
     6601 ttggcggacg tcttccgttc gatcaggtat cgcccttctt ctccggcgac attgacgtaa
     6661 ccggcgttct tcagcttctc gacgaagtcg gagaaactgc ctgagccata cgctttctcc
     6721 gtgaatgcgg aatcgagctg gagcatggtc gatttcagca ggccgagctg cggccgcaat
     6781 ccgcgccgct ccacaacctg gagcgcccgc tccactaggg gaaccgcttg gcccggatcg
     6841 agcggggcgg ggcggttccg tggctggcgc tgacggtgtg gcccctgatg ctgctgttgc
     6901 tgctgctggc gctgcggttt ctgtggctcg tccagcagcg attcgtagct gacgaactcg
     6961 tggcagttct gctgaagaat ggtgctggtg aacgccttgc cgccaaccac gaagaccgtc
     7021 ttgccgaact ccttcagctt gtttacaagc ggaatgaagt cgctgtcgcc gctgacgatg
     7081 gcaaaagcgt tgacgtggtt gtgggtaaag gccatctcga gcgcgtcgag ggccaggttg
     7141 atgtcggcgc cgttcttgtc cccgcgcgga gagggaatgc gctgtaccat ctggaccgcg
     7201 ttggcggcca tctggcgggt cgctccctcc tgtcgcgccc agttcgcata tgcaaacttg
     7261 gcgacgatat cgccacgctc cttcagggcg ttgagcgcca cggatacgtc gaattcacgt
     7321 tccagcgtcg atttgacgcc gatctcgatg ttgtcatagt cgacaaacac cgcgatgttc
     7381 aatctttctt gcattgggtt gtcttgtttc tctttgtaca ggcgtggtca ggctgcctgc
     7441 ttcaccgagg agcgtatgaa tgtcacaatc tcctcgagcg aagctcctgg ctgaaatacc
     7501 gccgccacgc cctgcttctt aagttgtacc gcgtcctcgt ctgggataat tccgccgacg
     7561 atgacaatga tatcgtccat ctccttctcg tgaaggagct ccatcaccct gggaacgatg
     7621 gcgttatgcg cgccggaaag gatggagagc ccgatcacct ggacgtcctc ctggagcgcc
     7681 gcgctggcga tcatctcggg cgtctgccgc agaccggtgt agatgacttc cataccggcg
     7741 tcgcgcagag tccgcgcgat gatcttagcg ccgcggtcgt ggccgtccaa gcccggcttg
     7801 gccacgagca ctcgaatcgg atgctccatc cctcgttccc cgtgattaca gcacgctggt
     7861 ttcgcgccag gttccaaaaa catcccggaa cgtgtcgcag atctcgccca cggtggcgta
     7921 gcagcggact gcgtcgagga ggaaaggcat ggtgttctcc cttccctcgg ccgcacgcct
     7981 cagcgcatcg agcgatgctt caacgcgagc ggcgtcccgc cgtttcctca actcagccag
     8041 tttctcacgc tgtcgaacct cggcgctacg gtcgatctcg aggatctcga tcggtttctc
     8101 gtcctcctgt tcgaaggcgt tgacaccgac gatgatccgg tcccctgttt cgaccgcgcg
     8161 ctggtaacgg tagctcgcct cggcgatctc actttgcggg aagttcctct cgatcgccgg
     8221 aatcatgccg cccatctggt cgatgcggcg gatgtattcc gcggccgatt gttcgacgtc
     8281 gatcgtcagc cgctcgagaa agtaacttcc ccccagcgcg tcgggaaccg ccgccacccc
     8341 actctcatgg gcgatcacct gctgcgtgcg cagggcgatc gtcacagcat gctcgcttgg
     8401 gagggcgtac gcctcgtcga gtgagttggt gtgcagcgac tgagcgccgc caagcaccgc
     8461 cgccagcgcc tgaagcgccg tccgcaccac attgttgtac ggctgctgcc aggtgagcga
     8521 gcatcccgcc gtctgcgcat gaaagcgtag cttgaggctc cgctcgtgct tcgcgccaaa
     8581 gcggtcccgc atgagatgcg cccacatctt ccgcgccg
//
LOCUS       DATIFQ010000267         2070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1811817, whole genome shotgun sequence.
ACCESSION   DATIFQ010000267 DATIFQ010000000
VERSION     DATIFQ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811817"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1942
                     /gene="ltrA"
                     /locus_tag="VLE22_12390"
     CDS             326..1942
                     /gene="ltrA"
                     /locus_tag="VLE22_12390"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HSB15251.1"
                     /translation="MGRGVVEDPGTSRNNMHENRETSRASTETVDRSAKAPSRNADMH
                     ALEESDCCVVPMKQPNKEGQPLAEAAEGRRQPKENATQSNTPPTQRGTRVSQGLSGVR
                     RAARERRQERFTALLHHVTVNLLRESFDALKKNAAPGVDGVTWREYETGLEGRLADLH
                     NRVHRGTYRAQPSRRVYIPKADGRQRPLGIAALEDKIVQQAVVTVLNEIYEVDFQGFS
                     YGFRPGRNPHQALDALNAGLQKKRVNWVLDADIKGFFDHVSHEWILKFLAHRVADNRI
                     LRLVQKWLKAGVSEDGEWSETTVGTPQGAVASPLLANVYLHYVLDLWVEVWRKKIACG
                     DVIIVRYADDLVAGFQHRADAERFLRDFQERLAKFGLEVHPEKTRLIEFGRFAASDRR
                     KRGQGKPETFTFLGFTHYCGRNSKGHFVVWRRTAAKRLRAKLLAIKQELRRRMHESIV
                     LVGAWLKRVVEGYYRYHAVPGNLAVLGRFRERICRYWRRTLRRRSQRRRPDWEQLRPI
                     FDRWLPRPRTLHPYPDVRFDARIQGRSRMR"
BASE COUNT          438 a          566 c          710 g          356 t
ORIGIN      
        1 gaacccctgg gatttaacgc tttcgcgcca gaatggatgg aatagaatgg aagcactcga
       61 gcggaggaca gggcaacgca gggatgcgag tgcgccggga taaaccggca tgttgtaggg
      121 gatgaaagag ccctacggga aaggagcagc gactccatct cgacctcgag tcttgcgcag
      181 tgcgccgcga ggcgtacggc gaagcgtaga cagaggcatc ggtgggccgg gcgattgagc
      241 cccgataact gacgaactgc gtgccgacac tgtttgctta gtgggaaggc aacatctgtc
      301 cgaggcgata tatcgcgagc cttgggtggg acgcggcgtg gtcgaagacc ctggcacgtc
      361 gagaaacaac atgcacgaga accgggagac ctccagagcg tctaccgaaa cagtagaccg
      421 gtccgcgaag gcgccgagcc gcaatgcgga catgcacgca ctggaggagt cggactgctg
      481 cgtagtaccg atgaagcagc cgaacaaaga ggggcaaccc ttggcggagg cggcggaggg
      541 aaggcggcag cccaaggaga acgccacgca atccaacacc ccaccgacac agcgcggaac
      601 acgggtgtcc caaggattga gcggcgtgcg ccgagcggcg cgggaaagga gacaagagcg
      661 gttcacggct ttgctgcatc acgtgacggt caatctgctg cgggagagtt ttgacgcatt
      721 gaagaagaat gcggctcctg gggtggatgg cgtgacgtgg cgcgagtatg agacagggtt
      781 ggaaggtcgg ctcgccgatc tgcacaaccg ggtccaccgt ggcacgtacc gggcacaacc
      841 ttcacggaga gtgtatatcc cgaaggccga cggaaggcaa cgaccgttgg gcattgcggc
      901 gctggaggac aagattgttc aacaggccgt ggtgaccgtc ctcaatgaga tctacgaggt
      961 ggactttcag ggcttttcgt atgggttccg gccaggacga aacccgcacc aggcgctcga
     1021 cgcgctcaac gcggggctgc aaaagaagcg cgtgaactgg gtactcgatg ccgacatcaa
     1081 gggcttcttc gaccacgtca gtcatgagtg gatattgaag ttcctggcgc atcgggtcgc
     1141 tgacaaccga atacttcggc tcgttcagaa atggctgaag gccggggtct cggaagacgg
     1201 cgaatggtcg gagacgacgg tgggtacgcc gcaaggggca gtggcttcac cgctgctcgc
     1261 caacgtgtac ctgcactacg tcctcgattt atgggtcgag gtctggcgga agaagatcgc
     1321 gtgtggcgac gtcatcatcg tccgctacgc cgacgatctt gtcgccggct ttcagcaccg
     1381 agcggacgcc gagcggttct tgcgagactt ccaggaacgg ctggcaaagt ttggtctgga
     1441 ggtccatccg gagaagacgc gactgatcga gttcggacgc ttcgcggcgt ccgatcgacg
     1501 gaagcgcgga caagggaaac cggaaacctt cacgttcctc gggttcaccc actattgtgg
     1561 ccggaactcg aagggccact ttgtggtctg gcggcgcaca gcggcgaagc gactgcgggc
     1621 taagctcctc gccatcaagc aggagttgcg ccggaggatg catgagtcga tcgtgctggt
     1681 cggagcgtgg ttgaaacgcg tggtggaggg ctactaccga taccatgcgg ttccgggcaa
     1741 cttggctgta ctcggtagat ttcgagaacg gatctgtcgc tactggcggc gcactttacg
     1801 tcgtcgcagt cagcggcgga ggcccgattg ggaacaactg aggccgatct tcgaccggtg
     1861 gcttcctcgc ccgcgaactc tgcatcctta ccctgacgta cgcttcgacg ctcgtatcca
     1921 agggaggagc cgtatgcggt aattccgccc gtacggatct gtgcgggggg tggtcagcga
     1981 tggtcatccc taccgcgacc ccgagcgctg actccggggc cagaccaggg cgggggcggc
     2041 gcaagccgcc ccgccggttt tgacttgact
//
LOCUS       DATIFQ010000268         6101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2193880, whole genome shotgun sequence.
ACCESSION   DATIFQ010000268 DATIFQ010000000
VERSION     DATIFQ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193880"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VLE22_12395"
     CDS             <1..926
                     /locus_tag="VLE22_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15252.1"
                     /translation="QMKLWAVLFAVAVVAAGQTEPQTEERRVQVIRHVAAGVQPAPGA
                     PGADVLRFVSSEFAWSDKLVKNAPYSAEAVTETTQILADGNRIVNKSSALVYRDSDGR
                     TRREQTLTHIGPWASQSPQTRVFLNDPVAGVGYILEPENQIARKIARPPRLPEEKGLT
                     EAAVPAEFHLPVPPPPQGEASNVMFVAAAGSTHSALSRGSAEYKTESLGKQSIEGVTA
                     EGNRTTTTIPAGEIGNERPIVTVSERWYSAELQTIVLTKQNDPRFGETTYRLTNIQRA
                     EPPQPLFEPPPGYTIQDQDKMIQMFMKKKDD"
     gene            complement(985..1341)
                     /gene="rnpB"
                     /locus_tag="VLE22_12400"
     ncRNA           complement(985..1341)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VLE22_12400"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1508..4471
                     /locus_tag="VLE22_12405"
     CDS             1508..4471
                     /locus_tag="VLE22_12405"
                     /inference="COORDINATES: protein motif:HMM:NF044381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSB15253.1"
                     /translation="MRHRHTLLLVLIISALGYTTTMSGQGLTTTAAKDDWEEINFEFN
                     SSILSDGYPSLLRLAELLGQNPDYKVTVEGHTDAIGSDRYNDKLARARSETVKSFLLK
                     YGAQANQVTTSGRGKREPKVDNRTKEARFINRRVVMTVRDGQGKIVSAGGVGDAIKAL
                     QEIAKKQEDCCNEILKKLDKLDEILAMLRDLKQENEGLKADVANLKQAQSGLKDQVAS
                     LPKPPEKAELAQIMETTAKKAIDESRPKRFALLGLNAGPDMTGNLSVTGKGRYFAPFG
                     EHHALQAEAEYLRYFDRQEGQFDVGLVNRYKNFQAGMFSSFKRVDMKEYSSGGTLGQA
                     AVAMDYLFSRGRIGMFGTKAFLDNPVIHRAGISRNIFEETYLSVVDQIGGSTQIGLHK
                     DAYIEGNLGALFRKGGSNRPGGMVRFVQPINPSWAFTMEAGLNETLVGVNNSGRVVFG
                     LQFGNWVRPKDFVGLNHPVPMDVPRVRYEVLKKRVRTGNDPPVADAGADQIGVAAGPI
                     TLDGSNSYDPDGDPITFEWAQTAGPQVPLSGMNTAQASFTAADGQSYTFRLMVKDDKG
                     GESRARVTVTTAAAPSVKIVRFTANPQSIRAGETTTIVWEVQNADEVSISGLGKVDPK
                     AGTSTVAPTQTTMYTLTAKNGTSEVSETLTVQVQRADARVLRFMATPTNINAGEAATL
                     TWETENATEVSISGVGAVAPNGSTAVSPTQTTTYTLTAKNPYGEASAAATITVAPGPR
                     IVRFTATPTEILPTEQSQLVWEVEGATEVTISGLGKVNASGTSTVSPPDTTTYVLTAK
                     GGQGDVTASAVVTVIKPAKILDFVADPPRIGQAGDATTLRWQTENADEVIITGVGSVP
                     VNGSVQVKPTSDVSYTLIAYGRRTQATALVIVRVATGGVNHPPVADAGPNAVTIDPQL
                     RLDGSRSYDPDGDPITFSWRNLGPRQAEISNGTTPIPVVKFMNGWGDYAFELTVTDSR
                     GASSTATVHIQYRDP"
     gene            complement(4533..5183)
                     /locus_tag="VLE22_12410"
     CDS             complement(4533..5183)
                     /locus_tag="VLE22_12410"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSB15254.1"
                     /translation="MIRVALPFGIGTPESKRGSYRDALNGAGIQPVENVTTLAGLDGL
                     LLAGGTDIDPALYGASRQPETDEPDQARDRLESALLHEALDRDLPVLAICRGVQLFNV
                     ALGGTLTQHLEGHRFPGRPEVHRIQIASGSRLESILGVDDYGVNSRHHQCVDKVASGL
                     VVTARAPDNVVEALELPGKRFVLAVQWHPEARTDGPDSQIFEAFRDAMRQGRAAGR"
     gene            complement(5285..6010)
                     /locus_tag="VLE22_12415"
     CDS             complement(5285..6010)
                     /locus_tag="VLE22_12415"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15255.1"
                     /translation="MTARVDEIAPDVHRICVYVPEYQLQFNHFLIKDDEPLLFHAGLR
                     GMFPLVREAVGRVLDPSKLRWIAFSHFESDECGALNHWLEMAPSAQAACSLVGTIVGV
                     DDYAIRPSRGLTDGDVLATGKYRFRFCSTKHLPHGWDAGLLFEETQRTLLCSDLFHHA
                     GDGEPVTSSEVLGRSKATILEYQKGPLADYVPYTPMTAEILKKLADLKPKTLAIMHGS
                     SFTGNGEQALLDLTTVFREAFGG"
BASE COUNT         1249 a         1891 c         1908 g         1053 t
ORIGIN      
        1 aacaaatgaa gctttgggcg gtactgttcg ccgtggccgt ggtggcggcc ggacagacgg
       61 agccgcaaac ggaagaacgg cgggtccagg tcatccgcca tgtcgccgcg ggggtccaac
      121 cggcgccggg tgcgccgggg gcggatgtgc tccgtttcgt ctcgagcgag ttcgcgtgga
      181 gcgataagct cgtgaaaaac gcaccgtatt cggcggaagc ggtcaccgag acgacccaga
      241 tactcgccga cggcaaccgt attgtgaaca agagcagcgc tctcgtgtac agggacagtg
      301 acggccgcac acggcgtgag cagacgctga cccacatcgg tccgtgggcg tcacagagcc
      361 ctcaaacccg ggtatttctc aacgatcccg tggccggcgt cggctacatt ctcgagcccg
      421 agaatcaaat cgcccggaaa atcgcgcgac cgcccagact gccggaagag aagggactga
      481 cggaagccgc cgtcccagcc gagttccacc tgccggttcc gccgcctccg caaggcgagg
      541 cgtccaacgt gatgtttgtg gcggcagcgg gctcgacgca ttcggcactc tcccgcggta
      601 gtgcggagta caagaccgaa tcactgggca aacaatccat cgaaggagta acagcggaag
      661 gaaatcgtac gacgaccacc atcccagccg gcgagatcgg taacgaaaga ccgatcgtga
      721 cggtttccga acgctggtac tcggcggaat tgcagacgat cgttctcacc aaacagaacg
      781 acccgcggtt tggggagacg acgtacaggc tcacaaatat ccagcgggcg gagccgccgc
      841 agccgctgtt cgaaccgccg ccgggttaca cgatccaaga tcaggacaag atgatccaga
      901 tgttcatgaa gaaaaaagac gactagcttg agcccctttg tcgcggctct gccgccgggg
      961 tggtgatcgc aggtgacttg ctgagaggcc cgccggacga taagccggtt tctgtaccct
     1021 cgcgggcggc agtcattcct ctgggcccgg cattactgcc gggctcttgc gacctacccg
     1081 agagtcgaac ggagcgggcc gctcctcccc tcctatttgg tcttgctccg cgtggggttt
     1141 accctgccag ccgaatcgct ccagccgcgg tgcgctctta ccgcaccttt tcacccttac
     1201 ctccccgaaa ccgggaaggc ggtgtatttt ctgtggcact ttccgtgatc cgtttccgga
     1261 cccccggccg ttagccggca cgctgcccta tggagaccgg actttcctcc cccttgcggg
     1321 agcgactgcc tgtccggaga acctctcggt tataattatc gcatttaatt tattagccgc
     1381 aagaattccg gggcttaaaa tccaccaatc cgtagtatat tcgattagtt acgggtaacc
     1441 ccgaagcttt gagaaatcgg cccggcaggt caacccgagc gagtatttta atggaggaga
     1501 gttgaaaatg cggcacaggc acacgctgct tttggttctc atcatctcag ccctgggcta
     1561 cacgacgacc atgtcaggcc agggcctgac gacgaccgca gcgaaggacg attgggaaga
     1621 gatcaacttc gagttcaatt catccattct gtccgacggc taccccagcc tgttgcggct
     1681 cgctgagttg ctgggtcaga accccgacta caaggtgacg gtagaggggc acaccgacgc
     1741 gatcggatct gaccgctaca acgacaaact ggcgcgggcg cgctcggaaa cagtaaagag
     1801 ctttctgctg aagtacggcg cacaggcgaa ccaagtgaca acgtcgggac gcggcaagcg
     1861 tgagccgaag gtcgacaacc gcacgaagga agcgcggttc atcaaccgtc gggtcgtaat
     1921 gacggtccgg gacggccaag gaaagatcgt gagcgccggc ggcgtcggcg acgcaatcaa
     1981 ggccctccag gaaatcgcca agaaacagga agactgctgc aacgaaatcc tgaaaaagct
     2041 cgacaagctg gatgaaattc tggcgatgct gcgcgacctc aagcaagaga acgagggtct
     2101 gaaggcggac gtggccaact tgaagcaggc gcaaagcggc ctcaaggatc aggtagcctc
     2161 gcttccgaag ccgccggaga aggcggagct ggcgcagatc atggagacga ccgcgaagaa
     2221 ggcgatcgat gaatcacggc cgaagcgctt cgctctgctg ggcctcaacg ccggtccgga
     2281 catgacgggc aacctgagcg taaccggaaa gggccggtac ttcgcgcctt tcggcgagca
     2341 ccacgcgctg caagcggagg ccgaatacct gcgttacttc gatcgccagg aagggcagtt
     2401 cgacgtcggt ctggtcaacc ggtacaagaa cttccaggcc ggcatgttct cgagtttcaa
     2461 gcgtgtggat atgaaggagt acagctcggg cggcactctc ggccaggcgg ccgtggccat
     2521 ggattacctc ttctcgcgcg ggcgcatcgg gatgtttggg accaaggcgt tcctggataa
     2581 cccggtgatc catcgcgccg gcataagccg caacattttc gaagagacat acctgagcgt
     2641 tgtggaccag atcggcggca gcacacagat cgggttgcac aaagacgcct acatcgaggg
     2701 caacctcggc gcgctcttcc gcaagggcgg cagcaaccgg cctggcggca tggtgcgatt
     2761 cgtgcagccg atcaatccga gctgggcttt cacgatggaa gcgggcctga acgagacgct
     2821 ggtcggcgtg aataacagcg gccgcgtggt gttcggactg cagttcggca actgggtgcg
     2881 gccgaaggat ttcgtcggcc tgaatcatcc ggtccccatg gacgttccac gcgtccggta
     2941 cgaagtgctg aagaagcggg tgcgcacggg caacgacccg ccggtggccg acgcgggtgc
     3001 ggaccagatt ggcgtggccg cgggcccgat cacgctggat ggctcgaatt cgtatgaccc
     3061 ggacggcgac ccgattacgt tcgagtgggc gcagaccgca ggcccacagg tgccgctctc
     3121 gggcatgaat accgcccagg cctcattcac cgcggctgac ggccagagct acacgttccg
     3181 cctgatggtg aaggacgaca agggcggcga gagcagagcg cgggtaaccg tgaccacggc
     3241 ggcggcgccg tcggtgaaga ttgtccggtt caccgcgaat ccgcagtcga tccgagccgg
     3301 cgagacgacc accatcgtgt gggaagtcca gaacgccgac gaggtgagca tcagcggcct
     3361 cgggaaggtg gatccgaagg ccggcacatc gacggtggcg ccgactcaga ccacgatgta
     3421 cacgctgact gcgaagaacg ggaccagcga ggtcagcgag acgctcactg tacaggttca
     3481 acgggccgac gctcgcgtcc tccgcttcat ggcgactcca accaacatca acgctgggga
     3541 agcggcaacg ctaacgtggg agaccgagaa cgccacggaa gtgagcatta gcggcgttgg
     3601 cgccgtggcg ccgaacggca gcacggcggt ctcgcccacg caaacgacca cctacacgct
     3661 gacggcgaag aatccgtacg gcgaagcttc ggccgcggcc acgatcaccg tggccccagg
     3721 cccgcgcatc gtccgcttca cggcgacccc aaccgagatc ctgcccaccg agcagtccca
     3781 gttggtttgg gaggtcgagg gcgccacgga ggtgacgatc agcggcctcg gcaaggtgaa
     3841 cgccagcggg acctctacgg tcagtccgcc ggacacaacg acttacgtgc tgaccgccaa
     3901 gggtggacag ggcgatgtca ccgccagcgc ggtggtgacg gtgatcaagc cggcgaagat
     3961 cctcgacttc gtcgctgatc cgccgaggat cgggcaggct ggcgacgcga ccacgctccg
     4021 gtggcagacc gaaaacgccg acgaagtgat cattaccgga gtcggttccg tcccggtgaa
     4081 cggatcggtt caggtgaagc cgacctcgga cgtgagttac acgctgattg cctacgggcg
     4141 gcgaacgcag gcgaccgcgc ttgtcatcgt tcgcgtcgcc accggcggtg tcaaccatcc
     4201 gccggtggcg gatgcggggc cgaacgccgt gaccatcgat ccacaactcc ggctggacgg
     4261 ctccaggtcc tacgacccgg acggcgaccc gatcacgttc tcgtggcgga atctgggtcc
     4321 gcggcaagcg gagatcagca atggcaccac gccgatcccg gtcgtgaagt tcatgaacgg
     4381 ctggggcgac tacgcgttcg aattgacggt caccgattcg cggggcgcga gcagtacggc
     4441 gaccgtccat atccagtatc gcgatccgta gttctcagtg aagtcgcgaa aaatcgaagg
     4501 gcgcgccctg cggcgcgccc ttttttgctt ggctatcttc ctgccgcccg cccctgtctc
     4561 atcgcatccc gaaaggcctc gaagatttgc gagtctggtc cgtccgtgcg cgcttctgga
     4621 tgccactgca ccgccagcac aaaacgcttt ccgggcaact ccagcgcttc caccacgttg
     4681 tccggcgccc tcgcggtaac cacgagcccg ctcgcaactt tgtccacgca ttgatggtgg
     4741 cgtgaattga caccgtagtc gtccactccc aggatcgatt ccagtctgct gccggatgcg
     4801 atttggatcc ggtgaacctc cggccgcccc ggaaatcgat gcccttcaag gtgctgcgtc
     4861 agcgtacctc ccagcgcgac attgaacagt tggaccccgc ggcagatcgc tagcactgga
     4921 aggtcgcggt ccagcgcctc gtgcaagagg gcgctttcga ggcggtcgcg cgcctggtcc
     4981 ggttcatccg tctcgggctg gcgtgaagcg ccatacaggg cgggatcgat atcggttcct
     5041 ccggccagca acaggccgtc gagcccggca agcgtggtga cattctcgac cggctgaatc
     5101 cccgcgccat tcagcgcgtc acggtacgac ccgcgcttgc tctccggtgt gccaattcca
     5161 aacggcagtg ccactctgat cacgcgcgcc cccccttctc ataccggaag aaatggggct
     5221 ggctgccatc tcgccgttgt tgcgaagtgg ctgtctgtcc ctattttttc gtgcccatct
     5281 ccccttaccc gccgaaggct tcccggaaga ccgtggtcag atcaagcaac gcttgctcgc
     5341 cgttcccggt gaaactcgag ccgtgcatga tggccagcgt cttcggcttc aggtccgcca
     5401 gctttttgag gatttccgcg gtcatcggcg tataggggac gtaatccgcc agcggtcctt
     5461 tctggtactc gagaatggta gctttgctgc gtccgaggac ctcagagctg gtgaccggct
     5521 ccccatcgcc ggcatggtgg aacaggtccg aacacagcag ggtccgctgc gtctcttcga
     5581 acagcagtcc ggcatcccaa ccgtgaggca ggtgtttggt ggagcaaaaa cggaagcgat
     5641 atttcccggt ggccagcaca tctccgtcgg ttaagccgcg ggaggggcga atcgcgtagt
     5701 cgtccacacc cacgatggta ccgaccagac tgcaggcggc ctgcgccgag ggcgccatct
     5761 cgagccagtg attgagcgcg ccgcactcgt ccgactcgaa atggctgaag gcgatccaac
     5821 ggagcttgga cggatccaac acgcggccga ccgcttcgcg caccagggga aacataccgc
     5881 gaaggcccgc atgaaacagc aaaggttcat cgtccttgat caggaagtgg ttgaactgca
     5941 actggtactc cggcacgtat acgcaaatgc gatgcacgtc cggcgcaatc tcatcgactc
     6001 tggcggtcac accgcacctc ccatccggga ttcctatgtt ggactcccga gtcttaacgt
     6061 agtgtatacc gatccgtctc tcttgtaggc gaaaagcgga t
//
LOCUS       DATIFQ010000269         5342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2004897, whole genome shotgun sequence.
ACCESSION   DATIFQ010000269 DATIFQ010000000
VERSION     DATIFQ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004897"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VLE22_12420"
     CDS             complement(<1..921)
                     /locus_tag="VLE22_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HSB15256.1"
                     /translation="MDEKQVPGGIPQDTESEGRRNFTEEAPKRGRAAGAAVPVAGIGA
                     SAGGLEVFRLLLADLPADTGLAIVFIQHLDPKHHSMLAEILARATTMPVSEAADSMPV
                     EANHVYVIPANVDLTIAYGALKLAPRTQTPGLHMPIDRFLRSAADQCGSRAIGVILSG
                     TGSDGSAGVEAIKAAGGVTFAQDAATAKFASMPQAAVATGCVDFVLPPERIASELVRI
                     GRHPYIADALRAQQERTPAGDEERFGAILAILHGATGIDFSLYREKMIKRRILRRLAL
                     RNIDGLAEYGKRLENDSGELMALQRDLLIGV"
     gene            complement(1159..1938)
                     /locus_tag="VLE22_12425"
     CDS             complement(1159..1938)
                     /locus_tag="VLE22_12425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB15257.1"
                     /translation="MGNSGENPVEGEVERLRKALDAETQKCLEVRRQLERASADFEEF
                     VSMAAHNLRESLRDVASFSQLMAETYAGRLDSDADVFLGRIQEGAARMQSLLTDVVDY
                     WATDAGGRQASRTDMEAVLRQALLSTDRQIKERNALVTHDPLPQVCGDFEILAKVLHH
                     LIRNAVEFCGTPPPRVHISSRREGLHWVFSVQDNGPAIDPAFEGRIFGVFKRLHGTEH
                     RGSGLGLAFCKKAIERHGGRIWMESTPGEGATFCFTLPPAD"
     gene            complement(1941..>5342)
                     /locus_tag="VLE22_12430"
     CDS             complement(1941..>5342)
                     /locus_tag="VLE22_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HSB15258.1"
                     /translation="ELLSRASKMPVAEVRGDILAEANHVHVIPPGRNLAISGGVLHTT
                     PRVDAGRNVPIDVFLEYLARDRGAKAIGVVLSGTASDGTLGLRAIKAAGGTTFVQEIG
                     SAKFDGMPRSAIAAGVADFVLPPAGIARQLAAIARDSHVPIEPQKATEQPGEAELKKI
                     FRLLRSATGVDFTYYKHSTLQRRIKRRMALRGFEKLEDYSRDIEQNRDEAKALCEDFL
                     ITVTAFFREPAVFEELKKEVFPALVENRGREDPIRIWVSGCSTGEEAYSIAICLMEFL
                     EKAELSLPFEVFATDISETAIEKARAGTYTGAALAHVSPQRLARFFTRTERGYQVTKT
                     IRDVCVFARHDVTQDPPFSKLDLISCCNVLIYLGAILQRKVLSILHYALKPTGFLVLG
                     PSENVGTLSESFHHVEKTHTIYHVLPAAGKPAAPISEDRRTKKVVDFRETIAEGRAGP
                     DVQREADRLMLAEHAPPGAIVDEDMNIVQVRGRTAPYLELSPGEPTHSLLKMAREGLV
                     AGLGKAFQTARQRNAVAKEDGMRIEGSGQLTEVTITVIPLRGSSSVKKRYFLVLFEDA
                     EPNGSPPTKRKPSTHDKDVTARLRRELAATKEYLQSILEDKAITLEELRSANEEAQAG
                     NEELETAQEELESANEELNTLNEELKISNAEFSQVNRDLTNLLESISIPLVMVGKDLR
                     IRRFTRAMEPMMNLIASDVGRSITDLQPQMELPDLRRLLLDAMEGGNRKPRDVRDSHG
                     RWFSLRILPSLGPDGKIDGAVVMLIDIDAAKRGLDFAEAIVETVREPLVILNQNLQVM
                     KANKTFYQTFQTSREETEQRLIWDLGNGQWNIPRLRELLENVLPAHSTFRDFEVTHEF
                     EHVGRKVMLLNASEIFDPNAQARTILLAIEDITDRKRAEEELKATNAEVQHFAYALTH
                     DLQEPLRMVVNFTELLAREYEGKLGEEADTIISYSVGGARRIEALLKALLGYWEVTER
                     DQDSFASIDCGAVLANVLANLEAAIAHSGAIVTSDSLPTVVAGEVMLMQVFQNLISNS
                     IKYRGEETPRIHVSAERDAEGWLFSVRDNGVGIAPKDADRVFGMFKRLHGSEIPGTGI
                     GLALCKKVVERQGGRIWVESERGRGATFKFTVPGRHREPEPRRAD"
BASE COUNT          995 a         1653 c         1530 g         1164 t
ORIGIN      
        1 cacgccgatg agcaaatccc gctggagagc catcagctca ccggaatcat tctccagacg
       61 ctttccgtat tccgcgagcc cgtcgatatt gcgcagggca aggcgccgca ggatgcggcg
      121 tttgatcatc ttttcccggt aaagtgaaaa gtcgatgccg gtggccccgt gcagaatggc
      181 caggatggcg ccgaaccgct cttcgtcgcc ggctggcgtt cgctcctgtt gcgcgcgtag
      241 ggcatccgca atataagggt gccggccaat tcttacaagt tcggaggcaa tgcgttcggg
      301 cggcagaaca aaatccacgc agccggtggc cacggcggct tgcggcatgg aggcgaactt
      361 cgcggtggcc gcgtcctgcg caaacgtcac gccgcccgcg gccttgatgg cttccacgcc
      421 cgccgagccg tccgagcccg tgccggaaag gattaccccg attgccctgc ttccgcactg
      481 gtcagccgcg gaccgcagga agcggtcgat gggcatgtgc aatcccggtg tttgcgtacg
      541 tggggccagc ttcagcgccc catatgcgat ggtcagatcg acgtttgccg gtatgacgta
      601 tacatggttg gcttccacgg gcatgctatc ggccgcctcg ctgaccggca tggtggtggc
      661 tcgcgcaaga atctcggcca acatgctgtg gtgctttgga tcgagatgtt gaatgaatac
      721 gatggctaag ccggtatcgg cgggcaaatc ggcgagcaga agcctaaaaa cttccaaccc
      781 gcccgcggag gcgccaatgc cggccactgg gacggccgcc cctgccgcac ggccccgttt
      841 aggcgcttct tcggtgaagt tgcgccgacc ctccgattcc gtgtcctgcg ggataccgcc
      901 gggcacttgc ttttcgtcca caaaacgccc ctttcaactc ctgaccaccg tacttacgct
      961 acgattttag aactcgcagt tcagcaattt gtgggccata acaaatcccg gcccaccggc
     1021 agcgaaagtc agccgaaatt tctggggaag caccacgatg aagagagtca gcgcgatgcc
     1081 ggcgccgggg ccgggatgaa aaggctgcac cggtaatgcg ggatttgccg acctggatcc
     1141 ggcgatcgcc ggttctgctc aatcggccgg aggcagagtg aaacagaatg tcgccccctc
     1201 ccccggagtc gattccatcc aaattcggcc gccatgccgc tcgatggcct tcttgcagaa
     1261 tgcgagtccg agtccgctcc cgcggtgttc tgttccgtgc aggcgcttga acacgccgaa
     1321 tatgcggccc tcgaatgccg ggtctatggc agggccgttg tcttgcacag aaaataccca
     1381 gtggagaccc tctcgtcttg atgaaatatg tacgcgagga ggaggcgtgc cgcagaattc
     1441 gacggcattt cgaatcagat gatgcaggac cttggccagt atttcgaagt ctccgcacac
     1501 ctgcggcaac gggtcatgcg tgacgagtgc gttcctttct tttatctgtc tgtccgtcga
     1561 gaggagcgcc tggcgtagga cagcttccat gtccgtccgg gaggcctgcc ggccgccagc
     1621 atccgtggcc cagtagtcca ccacgtcggt gagcagtgat tgcattctgg ccgccccttc
     1681 ctggatacgc cccaggaaca cgtcagcatc ggaatcgagg cggccggcat aggtctcggc
     1741 catcagttga ctgaacgagg ctacgtcgcg aagagactcg cggaggttgt gagccgccat
     1801 ggagacaaac tcctcaaagt ccgcgctcgc tctttccaac tgcctccgca cctcgaggca
     1861 tttctgcgtt tccgcgtcga gcgccttgcg caggcgttcg acctcgcctt ccaccggatt
     1921 ctcgccagag ttgcccattt tcaatccgcg cgcctgggtt cgggctccct atgtcggccg
     1981 ggaactgtga acttgaacgt agcaccgcgc cctctttccg actccaccca gatccgccca
     2041 ccctgacgct cgacaacctt cttacagagc gcaagaccga tcccagtacc gggaatttcg
     2101 cttccatgca agcgtttgaa catgccgaac acccggtcgg cgtctttagg agcgatgccc
     2161 actccgttgt cccgcactga aaacagccat ccttccgcat ctctctcggc cgagacgtgg
     2221 attctcggcg tttcctcgcc tctgtacttg atcgaattgg aaatgaggtt ctggaaaact
     2281 tgcatcagca tgacctctcc agctaccaca gttggcaacg aatcggaggt cacgatggcg
     2341 ccactgtgcg cgatggccgc ctcgaggttc gccagcacat tggcgagaac agcgccgcaa
     2401 tcgatcgagg cgaagctgtc ctggtcccgc tcggtaactt cccaataccc aagcagggcc
     2461 ttcagcaggg cctctatcct ccgcgcgccc ccgacggaat aggagatgat tgtgtccgct
     2521 tcttcgccca gctttccttc atactcccgc gccaggagct cagtaaagtt cactaccatc
     2581 cgtagaggtt cctgcaagtc gtgcgtgagc gcataggcaa aatgctgcac ttcggcattg
     2641 gtcgccttga gttcttcctc agctcgctta cggtcagtta tgtcctcaat cgcgaggagg
     2701 atcgtccgag cttgtgcatt cggatcgaag atctcgctgg cgttgagtag catcactttc
     2761 cgtccgacat gttcaaactc gtgtgtcacc tcaaagtccc ggaacgttga gtgtgcgggg
     2821 agaacattct ccagcagttc tcgcagtctc gggatgttcc attgtccgtt tcccaggtcc
     2881 catatgagac gctgctcggt ctcttcccgg gaggtctgga aagtctggta gaaggtcttg
     2941 ttcgctttca tgacctgcaa attctggttc aggatcacga gaggctcccg cacggtctct
     3001 acgatggctt cggcgaagtc aagaccgcgc tttgccgcat caatatcgat caacatcacc
     3061 acggcgccgt cgatcttccc gtctggcccc agggacggca ggatgcggag cgaaaaccag
     3121 cggccgtgcg agtcgcggac atcacgcggc ttccgattgc cgccctccat tgcatccaac
     3181 agtaaccgcc tcaggtcggg cagttccatt tgaggctgaa gatccgttat cgatcgcccg
     3241 acatcggagg cgatcagatt catcataggt tccatggctc gggtgaaacg gcgaatccgc
     3301 aggtctttgc caaccatgac caggggtatg ctgatgcttt cgagaagatt cgtcagatcc
     3361 cggttgacct ggctgaattc cgcgttactg atcttcagtt cttcgttgag tgtgttcagt
     3421 tcttcattgg ctgattccag ctcttcttgc gcggtttcca gttcttcgtt gcccgcctgc
     3481 gcctcttcgt tcgctgaccg aagctcttca agggtaattg ccttgtcttc gaggatcgat
     3541 tgcaggtact ctttggttgc tgcaagctcg cgccgcaacc gggcggtcac atctttgtcg
     3601 tgtgtcgagg gcttgcgctt tgttggtggg ctaccgttcg gctcggcgtc ttcgaacagg
     3661 accaggaaat accgtttttt gacagaggac gatcccctga gcgggatgac cgtaattgtg
     3721 acctcggtca actggccact gccttcgatt cgcatgccgt cttctttggc tacggcattt
     3781 ctctgtctgg cggtctgaaa cgccttaccc aggcccgcta ctaagccttc gcgcgccatt
     3841 ttcaagaggc tatgggttgg ctcgccgggc gaaagctcga ggtacggagc tgtgcggccc
     3901 cggacctgaa caatgttcat atcctcgtca acgatggcgc cggggggggc gtgttcggcc
     3961 agcattaacc ggtcggcttc tctctgcacg tccggcccgg ctcggccctc agcaattgtc
     4021 tcccggaaat ccaccacctt cttggtccgg cggtcttcgc ttatgggcgc cgccggctta
     4081 cccgcagccg gcagcacgtg atagatcgtg tgggtctttt ccacgtgatg aaaggactcg
     4141 gacagcgtgc cgacgttttc cgatggaccg agtacaagga agcccgttgg cttgagggcg
     4201 taatgcagga tcgacaacac cttgcgctgt aatatggctc caaggtagat cagcacgtta
     4261 cagcaactga tcaggtccag cttggagaaa ggcgggtcct gcgttacgtc gtgcctcgca
     4321 aaaacgcata cgtcacggat ggtcttcgtg acctggtacc cgcgttccgt tctggtgaag
     4381 aaccgcgcca atcgctgcgg cgacacatga gccaacgcgg cgcccgtgta tgtgccggcg
     4441 cgggcctttt cgatcgccgt ttcgctgata tcggtggcga agacctcgaa cggaaggctc
     4501 agttccgcct tctccaggaa ttccatcaaa cagatggcga tcgaataagc ttcctcgccc
     4561 gtggagcacc cagacaccca gattcggatt ggatcctcgc gccctctatt ctcaaccagc
     4621 gcggggaaca cctccttctt cagttcctcg aagaccgctg gttcccgaaa gaaggcagtg
     4681 acggtgatca ggaagtcctc gcacaacgcc ttggcttcgt cgcggttctg ctcgatatcc
     4741 cggctgtagt cctccagctt ctcgaatccg cggagggcca tgcgccgctt gatgcgccgc
     4801 tggagggtgc tgtgcttgta gtacgtgaaa tccactccgg tagcgctgcg caacagccgg
     4861 aatatcttct tcagttctgc ttcccccggc tgctcagtcg ccttctgagg ctcgatcggg
     4921 acatgggaat cgcgtgcgat ggccgccagt tgccgcgcga ttccggccgg cggcagcaca
     4981 aaatcagcta cacccgcagc gatggcgctt cgcggcatgc cgtcgaactt cgccgatccg
     5041 atctcctgaa caaacgtagt gccacctgcg gcctttatcg cccgtaggcc cagagttcca
     5101 tccgacgcgg tccccgagag aacgacgccg atggctttgg cgcccctatc gcgggccagg
     5161 tactcaagaa agacatcgat tggcacgttc cggccggcat ccactcgggg cgtggtatgg
     5221 aggactccac cggagatggc caaattgcga cccggcggaa tcacgtggac gtgattagcc
     5281 tcggcgagga tatcgcctct cacctcggcg acgggcatct tgctcgccct ggaaagcaac
     5341 tc
//
LOCUS       DATIFQ010000270         7835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_665131, whole genome shotgun sequence.
ACCESSION   DATIFQ010000270 DATIFQ010000000
VERSION     DATIFQ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665131"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..3460
                     /locus_tag="VLE22_12435"
     CDS             65..3460
                     /locus_tag="VLE22_12435"
                     /inference="COORDINATES: protein motif:HMM:NF006386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HSB15259.1"
                     /translation="MRGLLIAQFCGAFNDNAWKLMVALLAIRQATAHMAPGPELETAA
                     QTQTALTFIIFTLPLVLLSLVGGTLADRLSKRTVIIAIKIVEVLLMSAGTVALWLNPA
                     GGVLPLIVLCGMGVHSALFSPSKYGILPELIPHERLASGNGLLELWTFAAILTGTAAG
                     GFLLQSAGETAWLAPLTLALLSIVGLVAAFAVPRVPPARATGGVGSTIRGAWAAIQSE
                     RLLRLAIPGEIFFWTIASLFAQNVLVYAKAVLQLSDAMSGLPLTLLSVGIGLGAMLVG
                     RLSQNRVEYGLIPLGATGVFIALSLLGALTPPLWGTFLVMGLLGISSAFIFVPLNAVI
                     QWKSPPDRRGAVISFSNTCVFTGILLGSLAGGSLANAGLSTGSIFLATAGITIGGTLW
                     ALWLLPDAFLRLLLVILTNTVYRVRIVGQHHVPQSGGALLVPNHMSFVDGFLLMASVD
                     RPIRFVVDAAYATHPPFKALMTAMKVIPISSAGGPRMILRALRSAGQALDDGEIVCIF
                     PEGQITRTGTLLPFRRGFERIVKGRTVPVIPIHLDRVWGSIFSFTHGRFVSKWPERVP
                     YPVTVSFGAPQPADTPAYELRRLVRELGEAAWYLRKVDQEALHRPVVRTWRRNPFSFA
                     MADASRPHVSAFQALVGTIALARTLKPHWEGQEHVGLLLPPSVAGALVNIAASLAGKT
                     SVNLNYTVGRAGLESAVTQASIKTVLTSHRFIEKAKIEIPQGTAILWIEDIAKTISGG
                     RKVIAILFALFAPIRLVERACGQSRKRSMDDLATIIFSSGSTGEPKGVMLSHFSINSN
                     VEGAAQIIHISKTDRALGILPFFHSFGYMLLWFYARHNTGVVFHPSPLDVGVIGDLCS
                     RYRVSLLVVTPTFLQLYQRRCTPEQFSTLRVVLTGAEKLPLRLVQSFQERFGIAPVEG
                     YGVTECAPVIAANCPDFRAAGYYQVASRRGTVGQPLPGVSVRIVDPDTFELLSPGKPG
                     MLLVKGPNVMNGYLGRKDLTDKAMWDGWYVTGDIATLDEDGFLAITDRLSRFSKIGGE
                     MVPHGFVEEALQQAAGADMQVFAVTGIPDEKKGERLAVLHTLDESRIPDILDKVAAQG
                     LSNLFIPSPHQFVKVESLPVLGTGKIDLRGVKRIATERLMPAS"
     gene            3470..5173
                     /locus_tag="VLE22_12440"
     CDS             3470..5173
                     /locus_tag="VLE22_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15260.1"
                     /translation="MHNRLLQARTGLPDDSHTHRHSHATRLASVGAMLITTVVLGTHP
                     VWAAPESATVIGTIQIGSSPLPQEHLGLFNGTAFTLTTGRCNHCKTPPQALWYFAEES
                     IAVPSHPAAQEGQGSADPLPFLVWLGSPELISKALLASDHISLNVAEGSRIPLRLTPK
                     LPTNQSYYNHSTASFFSTHPLRLRGRTVPSPEGYYFETRTIWPADFLLTRDRSTDVTA
                     NNQTIPRLIEDAGQAASETITTHVLWEKSRASWAGKPVLGAILNGAQADDDEAHGGHF
                     GIVTGRVGPHGEWADWLVNNFYDPDVISEKGILPAMVPMDNYLMDLNSGQSYYRPSAL
                     LVIVLKHDRIPAVFQSTVQDVFRRLYRHELDYDNALLNCAGLSIDQLRALGWQIPMQG
                     PSSRLKATGGYLYIAATDRSLVSGLNVYRFFSEERTRLLPRVTFEAIGNDLLHLLQQS
                     DAGRMLTSFEQWLRDDVEAVLYVHIPQIPSSRAQGTHAVATLEEYQQRVPADHSKWKT
                     VAVPPRPFPAELRVSPAPVEATTIPHPVLALVIGFLLGMVWVGRLLFQRGNRGLRMRT
                     R"
     gene            5310..5918
                     /locus_tag="VLE22_12445"
     CDS             5310..5918
                     /locus_tag="VLE22_12445"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSB15261.1"
                     /translation="MPSIYQLKPAFQNLLRPVTRGLAAAGVTANQVTLAALGLSCLAG
                     AVIAWQPFNRPLLLLIPLTLILRMALNAIDGMLAREHDMKSRLGALLNELGDVLSDSA
                     LYLPLAQVPGFDPRLVVSVVVLSVIVELTGVAAVQIGAARNYAGPMGKSDRAFVFGLI
                     TLLLGLNISPGLWVTLLLGAMVLLSAFTIYNRARRSLAEGAV"
     gene            5918..6856
                     /locus_tag="VLE22_12450"
     CDS             5918..6856
                     /locus_tag="VLE22_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSB15262.1"
                     /translation="MLTHVKPPVFWALGGIIGILLLASVVTVLLKRLHPETHYLELHQ
                     RIRSWWIMVGIFAGAILLSRTASIVFFAFISFLALKEYVSLIPTRRADRRVLFWAYAA
                     IPIQYLWVHWEWYGMFIIFIPVYLFLLLPMRMVLIGETKEFLRAAGTLHWGLMMMVFS
                     VSHAAFFLVLAEDKNPIAGGAGLVLYLVFLTQFNDVAQYVWGKMTGHHKIIPKVSPNK
                     TWEGFLGGLATTIGLAALLAPQFTPLTLHESLLAGLLIGCGGFIGDVTISALKRDIGV
                     KDSGTLLPGHGGILDRIDSLTYTAPILFHFIYYLHY"
     gene            6866..7498
                     /locus_tag="VLE22_12455"
     CDS             6866..7498
                     /locus_tag="VLE22_12455"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSB15263.1"
                     /translation="MLQQLFFLTIVRPLVLIVLGLNVRHLDRIPVKGPAIIVSNHNSH
                     LDTLVLMTLLPRRLLRALRPVAATDYFLRDRLLAWFALTIMHIIPLERQAVERGQDPL
                     ASCSEALRNGELLILFPEGSRGKPEQMGSFKTGIAHLAKRHPDVPITPVFLHGLGKAL
                     PKGEALLVPFFCDVFVGEPVQWTGDRQSFMTTLETALNTLAAEGSFPAWD"
     gene            7535..>7835
                     /locus_tag="VLE22_12460"
     CDS             7535..>7835
                     /locus_tag="VLE22_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15264.1"
                     /translation="MQTAPSKTLVDCHVHLAALPDGDNGCFISPKLLKSPLFRFLLWK
                     HDLSPALPQESNRKYLDHLLAELRASRHVQKAVLLGMDGFYDRTGLLNRQHTEFLI"
BASE COUNT         1405 a         2601 c         2204 g         1625 t
ORIGIN      
        1 atttatacgc gcgtttgcga cagattcccg cacacatccc atgactcaag aacagaagca
       61 tcctttgcgc ggcctcttga tcgcccagtt ctgcggcgcg ttcaacgaca acgcgtggaa
      121 gctcatggtc gccctgttgg caatccggca ggccaccgcg cacatggcgc cgggccctga
      181 actggaaact gcggcgcaga cgcaaaccgc actcaccttt atcatcttca ccctgccgct
      241 ggtcttgcta tccttggtcg gcggcacgct ggccgatcgc ctgagcaaac gcacggtcat
      301 catcgcgata aagatcgtcg aggtgctgct catgagcgcc ggcaccgtcg cgctctggct
      361 gaatcccgcc ggcggcgtgc tgccgttgat cgtcttatgc gggatgggcg tgcacagcgc
      421 gctcttcagc ccgtcgaaat acggcattct ccctgagttg attccgcatg aacggctcgc
      481 ctccgggaac gggctcctgg agctctggac ttttgccgcc attttgaccg ggacggctgc
      541 cggcggtttt cttctacaga gcgccggaga aaccgcctgg ctggcgccgc tcacgttggc
      601 cctgctgtcc atcgtcggtc tggtcgccgc ctttgccgtc ccccgcgtcc cgcccgcacg
      661 cgccaccggc ggcgtcggtt caacgattcg cggcgcctgg gccgccatcc aatcagaacg
      721 gctgctgcgc ctggccattc ccggtgaaat ctttttctgg acgatcgcca gcctcttcgc
      781 ccagaacgtc ctcgtctatg ccaaggcggt cctccagctc tccgatgcca tgtcaggact
      841 cccgctcaca ctgctgtccg tcggcatcgg cctcggtgcg atgctggtcg gacggctgtc
      901 tcaaaaccgg gtggagtacg gactgatccc cttgggagcc acgggcgtgt tcatcgcgct
      961 gtctctgctc ggagcgctga ctccgccatt gtggggaacg tttctcgtta tggggctcct
     1021 cgggatctcc agcgcgttca tcttcgtgcc gctgaacgcc gtgatccagt ggaaatcacc
     1081 gcccgatcgg cgcggcgccg tcatctcatt ctccaacacc tgcgtcttca ccgggattct
     1141 gctgggatcg ctcgccggcg gctccctcgc caatgccggc ctttcgaccg gcagtatttt
     1201 ccttgccacc gccggaatca ccatcggagg cacactatgg gctctctggc tgctgcccga
     1261 tgcgttcctc cggctgctgc tcgtcatcct gacgaacaca gtgtatcggg tccgcattgt
     1321 cggccagcat cacgtgcctc aatccggcgg ggcgctctta gtcccgaacc atatgtcctt
     1381 cgtggatggc ttcctgctca tggcgagcgt cgatcggccc atccggttcg tcgtggatgc
     1441 cgcttatgcg acacacccgc ccttcaaagc gttgatgacc gcgatgaagg tcatccccat
     1501 ttcttccgcc ggcgggcccc gcatgattct ccgggcgctg cgcagcgcgg ggcaggcgct
     1561 cgacgacgga gagatcgtct gcatttttcc cgaggggcag atcaccagaa ccgggacgct
     1621 cctgccgttc cgaaggggat tcgagcgcat cgtaaagggg cgcaccgtcc ccgtcattcc
     1681 gatccatctc gatcgggtct ggggcagcat cttcagtttc acgcacgggc gattcgtcag
     1741 caaatggccg gagcgtgtcc cctatcccgt cacggtctcc tttggagcgc cgcaacccgc
     1801 cgacaccccg gcctatgaac tacgccgcct ggtgcgggag ctgggagaag cggcatggta
     1861 tttgcgaaaa gtcgatcagg aagccctgca tcgtcccgtg gtgagaacct ggcggcgaaa
     1921 cccgttttcc tttgccatgg ccgacgcttc ccgcccgcat gtgtcggcct tccaggcctt
     1981 ggtcggaacg atcgcccttg cccggacact caaaccccat tgggaaggac aagagcatgt
     2041 gggcctgctc ctgccgccca gcgttgcggg cgccttggtc aacatcgcgg cgtcgctggc
     2101 cgggaagacc agcgtcaacc tgaactatac ggtgggccgc gccggcctcg aatcagccgt
     2161 cacgcaagcg tccatcaaga ccgtgctcac cagccaccgc tttattgaaa aggcaaagat
     2221 cgagatcccc caaggcaccg ctattctgtg gatcgaggac atcgccaaga cgatcagcgg
     2281 cggcaggaag gtcatcgcga tcttgttcgc gctgttcgcc ccgatacggc tcgtggaacg
     2341 ggcctgcggc cagtcgcgca agaggagcat ggacgatcta gccaccatca tcttcagcag
     2401 cggcagcacg ggtgagccga agggcgtgat gctctcccac ttcagcatta actccaatgt
     2461 cgagggtgcc gcgcaaatca tccacatcag caagactgat cgcgctctcg gcattctgcc
     2521 gttctttcat tccttcggct acatgctgct ctggttctac gcgcgccata acaccggcgt
     2581 ggtcttccac ccctcgccgc tcgacgtagg cgtcatcggg gacctgtgca gccgctaccg
     2641 cgtctcgctg ttggtcgtta ccccgacctt tctccagctg tatcagcggc gctgcacgcc
     2701 ggaacagttc agcacgctcc gcgtggttct cacgggggcc gaaaagttgc cgctgcgtct
     2761 cgtgcagtcg ttccaggaac gattcggcat tgcccccgtc gaaggctacg gggtcacgga
     2821 gtgtgcgccc gttattgcgg cgaactgtcc ggacttccgc gccgccggct attaccaggt
     2881 ggcgtcgcgc cggggaaccg tcggacagcc cctgccgggc gtgtccgttc gcattgtcga
     2941 tcccgacacg tttgagcttc tctctccggg caaaccaggc atgctgctcg tgaagggccc
     3001 gaacgtgatg aacgggtatc tgggccggaa ggatctcacg gacaaagcca tgtgggacgg
     3061 ctggtacgta accggcgaca tcgccacgtt ggacgaagac ggtttcctgg cgatcaccga
     3121 caggctctcg cgcttctcga agatcggcgg ggaaatggtt ccccacggtt tcgtcgaaga
     3181 agcgctgcag caggctgcgg gcgcggatat gcaggttttc gccgtcacgg gcattcctga
     3241 cgagaagaaa ggcgagcgtc tggccgtcct gcatacgttg gacgaatccc gcatcccgga
     3301 catcctggac aaggtcgcag cccagggatt atcgaacctc ttcattccat cgccccatca
     3361 gttcgtgaaa gttgagtccc tgccggtcct cggcaccggc aagatcgatc tccgcggcgt
     3421 gaagcgcatc gccaccgagc ggcttatgcc ggcgtcgtga cgtctcccta tgcacaaccg
     3481 actgcttcag gcgcgaacag ggttacccga cgattcgcac acgcaccgcc actcgcatgc
     3541 gaccaggctt gccagtgtcg gcgccatgct gatcaccact gtcgtgcttg gcacccatcc
     3601 tgtgtgggcc gcaccagaat ccgcgaccgt gataggaacg attcagatcg gaagttcccc
     3661 gctgcctcaa gagcatctcg ggctcttcaa tggcaccgcc ttcacgctga caaccggccg
     3721 atgcaaccac tgcaaaacac ctccgcaggc tctttggtac ttcgctgagg agtcaattgc
     3781 agttccctca caccctgctg cgcaggaagg ccagggctcc gcagatcctc taccatttct
     3841 ggtgtggctc ggctcacctg aactgatctc gaaggcgctg ctcgcatcag accacatctc
     3901 cctcaacgtc gcggagggca gccgcattcc tcttcggtta acgcccaaac tcccgacgaa
     3961 tcagtcgtac tacaaccact ccactgcgag ctttttcagc acccatcccc tgcgcctgcg
     4021 cggccgcacc gttccctctc ctgagggata ttacttcgaa acccgcacca tctggccggc
     4081 cgatttcctg cttacccgcg atcgatccac ggatgtgaca gccaataacc agaccatccc
     4141 gcgattgatc gaagatgccg gtcaggccgc gagtgaaacc atcacgaccc atgtgttgtg
     4201 ggagaagagc cgcgcatcat gggccgggaa accggtcctc ggcgccatac tcaatggagc
     4261 ccaagccgac gacgacgagg cgcacggcgg acacttcggc atcgtcacag ggcgagtcgg
     4321 tccccacggc gaatgggccg actggctggt gaacaatttc tacgatcccg acgtcatcag
     4381 tgaaaagggc attctccctg ccatggtccc gatggacaac tacctgatgg atctcaatag
     4441 cggacaatcc tactatcggc cgagtgcgct ccttgtaatc gtgctgaagc acgatcgaat
     4501 tcccgccgtt tttcaatcca ccgttcagga cgtgtttcgc cggctctacc gccacgaact
     4561 ggattacgat aacgccctgc tcaactgcgc agggctcagc atcgatcaac tgcgtgccct
     4621 cggctggcaa attcccatgc aaggccccag cagtcgtcta aaagcgacgg ggggctatct
     4681 ctatatagcc gccacggacc gcagcctcgt cagcgggcta aacgtctatc gatttttctc
     4741 tgaggagcgg accaggctgc tcccacgcgt gaccttcgag gcgatcggaa acgacctctt
     4801 gcacctcctt caacagtcgg acgctgggcg gatgctcacg tccttcgagc aatggctgcg
     4861 tgacgatgtt gaagccgtgc tctacgtcca tatcccgcag attccatcca gtcgcgccca
     4921 aggcacccac gcagtcgcga cactcgagga gtaccaacag cgcgtccccg ccgaccactc
     4981 gaaatggaaa accgtggcag tcccgccgcg cccgtttcca gctgagttgc gcgtgagccc
     5041 cgcgccagtc gaagccacga caatcccgca cccggtactc gcactcgtga taggtttttt
     5101 actgggcatg gtctgggtgg ggcgacttct ctttcagcgc ggcaaccgcg ggctgagaat
     5161 gcgcacacga tgacaaccaa ggagccgggc ttgaccacca agtccctggt caattgtcag
     5221 gctaccgtct caatcaaacg agatttgact agtcaatcaa acgcgttgtg ctacgcttgg
     5281 ttcagccctg ctgggatact taccgggcca tgccgagtat ctatcaactc aaaccggcct
     5341 ttcagaattt gctgcgtccg gtgacgcgcg gcctcgccgc cgcaggcgtg acggcaaatc
     5401 aagtaaccct cgccgcgctg ggcctttcct gtctcgcagg tgcggtcatt gcctggcagc
     5461 cgttcaaccg tccgctactc ctgctcatac cgctcacgct gatcctccgc atggcgctga
     5521 acgcgatcga cggcatgctg gcccgtgagc atgatatgaa gagccggctg ggcgcgctcc
     5581 tgaacgagct gggagacgtc ctctcagact ccgccctgta tctgcctctg gcgcaggtgc
     5641 ccggcttcga tccgcggctg gtggtgtcgg tcgtggtcct gagtgtcatc gtggagctga
     5701 ccggcgtcgc cgccgtccag atcggcgctg cacgcaacta tgccgggccc atgggcaaaa
     5761 gcgatcgtgc gtttgtgttt ggcctcatca cgctgctcct gggcctcaac atttctcccg
     5821 gcctctgggt gaccctgttg ttgggtgcga tggtgctgct ctccgccttc accatctaca
     5881 atcgcgccag gcgttcattg gccgaaggag ccgtatagtg ctcacccacg tcaaaccgcc
     5941 ggtcttctgg gcactcgggg gcataatcgg cattctcctt ctcgcctccg tcgtcacggt
     6001 tcttcttaag cggctgcacc cggaaacaca ctatctcgaa ttgcatcagc gcatccgttc
     6061 ctggtggatc atggtcggca tcttcgccgg ggccatcctc ttgagccgga ccgcctccat
     6121 cgtcttcttt gccttcatca gctttctcgc gctgaaagag tatgtctcac tgattcctac
     6181 cagacgggcc gaccggcgcg tgctcttctg ggcctacgcg gccattccga tccaatacct
     6241 ctgggtgcac tgggaatggt acgggatgtt catcatcttc attccggtct atctgttcct
     6301 cttgctgccc atgcgcatgg tgctgatcgg cgaaacgaaa gaatttctgc gcgcagccgg
     6361 cacgctgcac tggggtctga tgatgatggt cttcagcgtg agtcacgcgg ccttcttcct
     6421 cgtattggcc gaagacaaga acccgatcgc cggcggcgcc ggcctggtcc tctatctcgt
     6481 cttcctgaca caattcaatg acgtggctca gtacgtctgg ggcaaaatga cggggcatca
     6541 caaaatcatc ccgaaggtca gtcccaacaa gacctgggaa ggatttctgg gcggcctcgc
     6601 gacgacgatc ggtctggctg cgctactggc gccgcagttc accccgctga cgctgcatga
     6661 atctctcctg gcgggcctgc tcatcggatg cggcgggttc atcggcgacg tgacgatctc
     6721 cgcattgaag cgcgacatcg gcgtgaagga cagcggcacc ctgctaccag gacacggcgg
     6781 cattctcgat cgcatcgaca gcctgaccta taccgcaccg attttgtttc acttcatcta
     6841 ttacctgcac tattgagagc gcgtcgtgct gcagcaactc ttctttctga caatcgttcg
     6901 tccccttgtc ctgatcgtgc tgggcctgaa cgtccggcac ctcgatcgga ttccggtgaa
     6961 ggggccggcg attatcgtct ccaaccacaa cagccacctg gacaccctcg ttctcatgac
     7021 gctgcttccc cggcgcctgt tgcgcgcgct gcgaccagtt gcggcaacag actacttcct
     7081 gcgggaccgc ctgctggcct ggtttgcact gaccatcatg cacatcattc ccctcgagcg
     7141 tcaggcggtg gagcgtggcc aagaccccct ggcctcctgc tccgaagcgt tgcgcaatgg
     7201 cgaactcctg attctctttc ctgaaggttc tcgcggaaag cccgagcaga tgggcagctt
     7261 caagaccggc atcgctcacc tcgccaagcg acatccggac gtgccgatca cgccggtatt
     7321 cctgcacgga ctcggaaagg cgctgccgaa aggcgaagca ctgctcgtcc cgtttttctg
     7381 cgatgtgttt gtgggcgaac cggtacagtg gaccggcgac cgtcagagtt tcatgacgac
     7441 gctcgaaaca gcattgaata cattggccgc ggaaggctcg tttcccgcct gggattaagt
     7501 gatagactgc ccaagcgacc gctggagatc gcccatgcag acagcaccga gcaagaccct
     7561 tgtcgactgc cacgtgcatc tcgcggcgct ccccgacggc gacaacggct gctttatttc
     7621 gccgaagctg ctcaagagcc cgctatttcg cttcctctta tggaagcatg acctctcgcc
     7681 ggcgcttccg caggaaagca accggaaata cctcgaccat cttctcgccg agttacgcgc
     7741 ctcccggcat gtgcagaagg ccgtgctcct cggcatggac gggttctacg accggaccgg
     7801 tctgctcaac cggcagcaca cggagtttct catca
//
LOCUS       DATIFQ010000271        43532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2388133, whole genome shotgun sequence.
ACCESSION   DATIFQ010000271 DATIFQ010000000
VERSION     DATIFQ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 43532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43532
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2388133"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..839)
                     /locus_tag="VLE22_12465"
     CDS             complement(132..839)
                     /locus_tag="VLE22_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB15265.1"
                     /translation="MDGRLHTTLRGDGIVAEFIEILNGYVASRYSRGKEGALTPVNEK
                     GIREKVRDAYSAAARDPLAMHPFPVGRSFAESLGYPPELLVPLPAESVEAFSGVSNVS
                     LFAEIPRGSVVLDLRCGAGLDSVVAAGRTGPAGRVIGIEFSEEMSGRARRSAAAAGFR
                     NCLVCEADAERLPIRDNTVDVALANGIFNLNPARKAILQELARVLRPGAAVFGAELIL
                     SQPLPAEQVMTAADWFA"
     gene            complement(1070..1435)
                     /locus_tag="VLE22_12470"
     CDS             complement(1070..1435)
                     /locus_tag="VLE22_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15266.1"
                     /translation="MELQTEDPRSPDVRVGKIFDRNWELLSWLVPMALVRGTLLKHYH
                     HKVEDLVFTNLSRLASQWEDIVNASLLALEKESIRRLGGLIGTIEKLIASAGQQTQQL
                     RVDIERLAELRHRLNGGGA"
     regulatory      2054..2123
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2173..3459
                     /locus_tag="VLE22_12475"
     CDS             2173..3459
                     /locus_tag="VLE22_12475"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB15267.1"
                     /translation="MFKNLLTAASLLAATVTCAPAQQQPPQALTLQDCIRLAESVPSS
                     VSVALQERDIAARDVTQARAGFLPQSRLINGFTYNSPLMNNRETFSFIPLNGIREYAT
                     LFSVNQEFDTSGRLRADMTRARVAQEIAATSVALTQRDLRRAVTTAYYRALLTRRIVR
                     IIEDALAESRSFESRAKLLFQGGEAAQADVVKASAQVAFLQQAVNAAELEANLASQEL
                     ASFWTRDVTGSLALADLFDESPPPPEPEAVQAAPFLRRLEFSLFDAQRRGFQADVRRA
                     RSALLPQLGFTFQYGIDSTAVRISDRGYAAFVNLTIPVFDWNRARSTMQQARLRTQQI
                     ESTRAISERTFSKDYQGALARVKQLFAQISLTSQQAKLAEDDLRLSRIRYEGGEGSAL
                     DVVTAQNQLAQARSNYYATMANYLNAKADLEVAAGR"
     gene            3456..4691
                     /locus_tag="VLE22_12480"
     CDS             3456..4691
                     /locus_tag="VLE22_12480"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB15268.1"
                     /translation="MKPQNALLLSLFVLVGCGSHEDLPPKPVVAVVVAKADVGDVKSM
                     VRAPAFVFAREQANIGSRVTAPIRELLVRKGDSVGAGQVLAQLDNRDLLAQRDEAAAA
                     VTEAEANLERVTSGTLPTDIERARGQSATTEAALNQAQKFYDRRRQLFEQGAIPQRDL
                     LLSETELAQAKANHEVATRALDLLQNQSRDKDILMAKSKVEQARAHLAAIKAQLGFTE
                     IRSTSPGTITEQFMFPGDMAKPDAPIFTVMDLAVAVARAQIPDSEVAAVRTGQTCAFV
                     PADAAGTSFAGRISVVNQSIDTARRTVESWCEIPNPNRALRAGTFGQVSIVTGIVPKS
                     VVVPVAAVQFAEGASKGVVMVAGDKGVAVKREVETGDVFEGKVQIRNGLNAGESVIIQ
                     GAYGLADGTQIRVQEGKKP"
     gene            4688..7759
                     /locus_tag="VLE22_12485"
     CDS             4688..7759
                     /locus_tag="VLE22_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB15269.1"
                     /translation="MTGRLAQFVQANARAMVIIVLAFALAGVAFMFRLPIAIFPQTDF
                     PRIVILVDNGITPVDIQMLRVTRPIEETIRIVPGITTVRSVTARGSTEISVFFRWDVD
                     ILNALHLVQGRISQITPSLPSTCRFYINRLTFSIFPMVGFSITSPTRSQANLWELAYY
                     NIAPRLYRLPGVAETRIVGGRPPEYHVLVDPQKLNSYGMPLTKVTDALRSSNTISPSG
                     MVQENYHLYLTTVTGLLRTREQIEDTVVDVVRGTPVAVKNLANVVPGERPIYNIVTAN
                     GRPAVLVNVLQQPDGNAVQIADSVNAELSDIKKALPPDIELSTYYDQSVLVRDSIAGV
                     TESILIGLGLSVLVLLVFLKSWRTTIVAAVVIPIAVLIAIVFMKFFKMSFNLMTLGGL
                     AACIGVVIDDAIVMVENIIVHLSTGQAPMEAAKSAITELTPALTGSTLTPIMVFAPLV
                     FLGGITGVFFRALAMTMVTALLASLFLAIFFTPVLAAVFFRRSTGAAATDLEQAEQAG
                     EGRVLRWLTARYEAGLHWSLYHPRLILLSTVVVLAGMIGVYFRLGSGFLPEMDEGAFV
                     LDYRTPAGTSLQETDRVLRHIEQFVKETPEIESYSRRTGARLALAIAEPNTGDFLIKL
                     KKDRRRSLEDVTSELRHKIVSSEPVIDVEFPHILEDLIGDLAWSPQPIEIKVRHDDAE
                     TYKEVARRIEGWLGKVKGVVDIVNQTIVIGPSHNFRVDPEKARLAGFGVKDVADLQAA
                     MLDGELASDLIRGDRLVGIRVRYPAEYRSSTDKLKSLLLTSPTGQTVPMSSIATVEVD
                     EGTTEIRRENLRNMSAVTARLEKRDLGSAIKEIQQRLPKEIALPPGTHIEYGGLYEMQ
                     RESFLGLTQVLLLSILLIFVILVFEFRSFSHPIAILAATILCGSGALVALWVTRTTLN
                     ISSFMGAIMVVGIVHKNGILMLDSEQYFSEKGYELREAIFHAGRRRLRPILMTALATI
                     FGMAPLAVGVGSGAQLLQPLAIAVIGGVAVSMVLSLLITPVLFFQLRQRGL"
     gene            7845..8214
                     /locus_tag="VLE22_12490"
                     /pseudo
     CDS             7845..8214
                     /locus_tag="VLE22_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8229..8540
                     /locus_tag="VLE22_12495"
     CDS             8229..8540
                     /locus_tag="VLE22_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15270.1"
                     /translation="MWANQYPVVLLEDGMGENDWEGWRDLTVTLGSRIELVGDEVFCT
                     NPRILQEGIENGIANAILIKLNQIGSVTETLDTIELAGRNSYRCFVSHRSGETEDTSG
                     A"
     gene            complement(8561..9775)
                     /gene="ispG"
                     /locus_tag="VLE22_12500"
                     /gene_synonym="gcpE"
     CDS             complement(8561..9775)
                     /gene="ispG"
                     /locus_tag="VLE22_12500"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSB15271.1"
                     /translation="MAGRPRRKAVVCDVGGVQVGGSHPIVVQSMTNTDTADVASTVNQ
                     VMELARAGSELVRVTVNTEAAAAAVPKIVESLAKLWVSVPVVGDFHYNGHILLKKYPD
                     CARALAKYRINPGNVNIGKKHDDNFRTMIEVAIENAKPVRIGVNWGSLDQVLLTRLMD
                     ENSRQSEPLDAATVMLRAMVQSALESAQAAERYGLGHDRIILSAKVSGVQDLIEVYRM
                     MAGECDYPLHLGLTEAGLGSKGIVASSAAMSVLLQEGIGDTIRTSLTPLPNGDRSEEV
                     IVSQQILQSLGLRNFSPQVTACPGCGRTTSTFFQEMADRIQSYLREQMPVWKGRYPGV
                     EELKVAVMGCVVNGPGESKHSHIGISLPGTFEEPTAPVYVDGRLHTTLRGDGIVAEFI
                     EILNGYVASRYS"
     gene            9858..10814
                     /locus_tag="VLE22_12505"
     CDS             9858..10814
                     /locus_tag="VLE22_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB15272.1"
                     /translation="MTLTRLWLTVVSAALAAVALIVPPGGAQTGALKEVVPGIWFREG
                     DLKELGHCNNVIIEMKDYLVVVDANFPSGARLVLADAKKVSSKPVKYVFDTHHHGDHA
                     YGNPVWTKMGAVTIAHVNVAEELKRYEPKRWLETARTRQDVAELNLPEPEPPRQTFDK
                     SPYVIEDGRRRIELHFFGWAHTRGDGFAYLPKEKVLCTGDAAVNGPYNYTGDASILNW
                     PNVLRAAQKLDVVHVLPGHGGLAGKELLTGQAQFFIELRKAVDAAVKKGKKVDDLVTM
                     KDGNPAATTVKLPESVKTWVGDSLPAQVRDAYVEITRNSSRP"
     gene            10971..11330
                     /locus_tag="VLE22_12510"
     CDS             10971..11330
                     /locus_tag="VLE22_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15273.1"
                     /translation="MNGPRNPMKVLFSGLDAQLAKELGAALVEAGGCIRVADRVTADV
                     IFCAADPVCYRAMLEILRREQPGLPVIVVSRHPRASEWIDAIEAGVTDYCATPFEPTQ
                     LQWLLETSRHARRAAAA"
     gene            11377..12354
                     /locus_tag="VLE22_12515"
     CDS             11377..12354
                     /locus_tag="VLE22_12515"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HSB15274.1"
                     /translation="MSIRLFLRLAVHTQMLPVLEAGEETLVGGQAVMEGVMMRAPHSY
                     CVAVRRPAGDMIKDEMPVPRVSEKYPAFKYPILRGVGTLGQAMWLGIKALRFSANAAL
                     ENEKAGEKKQELSPWLMTLNLAFSIAFFIFLYKFVPLLLTSALGKWFPAVEGRIVFNL
                     TDGLIRIAIFLTFLFLISLWKDIRRVFEYHGAEHKVVFNYESGRPVTVENAQSFTTFH
                     PRCGTSFLLVVMVIAIFVYALVPYDSFLMKFVARVALLPLIAGLSYELIRFAAKHRGG
                     LMATLTAPGLWLQRITTQPPSNDQVAVAIHALEGAMALENAQGGQAVIA"
     gene            12364..13494
                     /gene="prfA"
                     /locus_tag="VLE22_12520"
     CDS             12364..13494
                     /gene="prfA"
                     /locus_tag="VLE22_12520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HSB15275.1"
                     /translation="MQYAQKLDEIEARFEELTRQMADPAVISDSENYRKTTKAHSELE
                     EVVTKYREWKRAESNLREARPMLEESDPELRTMAEEEVARLEPKLERLEQELKVLLLP
                     KDPNDDKNVLLEIRAGTGGDEATLFAAEIFRMYTRYAESQGWKVEISSVSDSAVGGVK
                     EVIALVNGDKVYSKLKYESGVHRVQRVPVTEQQGRVHTSAITVAVLPEADEVEVQIDP
                     KDIRIDTFCSSGPGGQSVNTTYSAVRITHLPTGLIVSCQDEKSQIKNRAKAMRVLRSR
                     LYEIELEKQQAQIGAERRSMVGTGDRSEKIRTYNFPQNRVTDHRIGLTLHQLDLIMDG
                     RLDVIIDALSTYYQAEKLKQQTAPAGAPHDSSHRTPAGVQAS"
     gene            13451..14287
                     /gene="prmC"
                     /locus_tag="VLE22_12525"
     CDS             13451..14287
                     /gene="prmC"
                     /locus_tag="VLE22_12525"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSB15276.1"
                     /translation="MTVHTALRQGSRLLEEGHVPAPRLTAEVLLARALGCERGRLYSH
                     PEEELSDLARLHYGRYLHERLRGKPTQYITKRQEFYGREFEVTPEVLIPRPETEHVVE
                     TALAHSSRGRRVVDVGCGSGAIAVTLRLETGAEVWGTDISQVALVVAARNASRLDAAV
                     HLIVCDVLDAVASRSVDLVVSNPPYVALHEAPGLQREVRDHEPHVALFAGETGFEIYE
                     RIVADGARVLNPGGRLVLELGFKSCDRVRGMLAAGWEDIEIIPDLAGIPRVISARWAG
                     AK"
     gene            14294..15547
                     /gene="dinB"
                     /locus_tag="VLE22_12530"
     CDS             14294..15547
                     /gene="dinB"
                     /locus_tag="VLE22_12530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HSB15277.1"
                     /translation="MRTIFHVDMDAFFVSVEELFDPSLKGKPVVVGGRPDQRGVVSAA
                     SYAVRKYGVHSAMPLRTAYKLCPHAIFVDGHPHRYREYSDKVFRVLGSFTPQVEMASI
                     DEAYLDMTGTERLYGPPLRAAHLLHDRVRAETQLNCSIGIGTSRLVAKIASDQAKPNG
                     VLWVLPGQEARCLAALDVRKIPGVGKATEQQLHRLGIRKVGDLAKLDDSFLEEKFGKW
                     GLALAGKSRGLDAGGWFDGVIGDEEDPKSISHEHTFDEDTADLERLESTLARLSEMVG
                     RRLREHGLYARTVQLKLRYKDFTTITRARTLDHATQLDTELFDEARSLFAGNWKRGVP
                     VRLLGVQASSFDSGEGQMNLLEAEKNERWRKALSAADRLRDRFGESAVSLATGMKGGF
                     RERVHENPAGLPGKEPKGKKNPRDV"
     gene            15648..16115
                     /locus_tag="VLE22_12535"
     CDS             15648..16115
                     /locus_tag="VLE22_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15278.1"
                     /translation="MRCLLAVAAGFFLIGIPLAADRENGQGNDEGNHRHKHHYKGRYK
                     DYDGPSAVAVNIFVGHDQRIVGDFLMGRPGSGLPPGLAKRGGDLPPGLMKQLYRKGHL
                     PPGLEKKIYPFPVDVEQRLSPLSPGLARGYIGGRAVIFNRKTSAIFDVFVPLD"
     gene            complement(16148..16354)
                     /locus_tag="VLE22_12540"
     CDS             complement(16148..16354)
                     /locus_tag="VLE22_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15279.1"
                     /translation="MDTGTPHNKRFLDLHYGRVTPGGAIKAHNANDFEHRQPDYLKAI
                     TTDPNLETKITRTPTGGISVSIRK"
     gene            complement(16395..17438)
                     /locus_tag="VLE22_12545"
     CDS             complement(16395..17438)
                     /locus_tag="VLE22_12545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB15280.1"
                     /translation="MRSEIVRCQLALFVVLAAPALAGPDHPSAEQLVERHLRAAGGRE
                     AAGRITTQIHRGTWHPRPDAPGFPVEVYGKAPGKWDYRMTIPGVLMIERACDGVSAWT
                     QTPDGTETVKPDDLVSENHGFDFQVPFRLTELFPDMTVKSREKSGSHEVWVVEFKPAQ
                     GKAVVAHFDAGTGLLSRLGIFLFEDYRLVDGVQVPFTVRVLEGGREQVMKFTEIQHNV
                     DVDDSRFVLASNTAAYKRAMSEMMRDSLDRSLEGIDPGAARTVLDRLHGFSPQDGRIL
                     YDLIVEKGYKQGLEIGTAEGNSAIWMGMAFKRNGGKLITLEMNAERAKAAAENFREAG
                     LDSVVECRVNDAF"
     gene            17524..19110
                     /locus_tag="VLE22_12550"
     CDS             17524..19110
                     /locus_tag="VLE22_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15281.1"
                     /translation="MTKLVEKLEEAKRRFGPGEAQRTEALLNEAAKARVIEADELIRF
                     HEALLFHRAYPKSEGVLQRAEDLLASFGERAARADPEPFEEPEVSGIAGTAFSAIFSY
                     DVARRLVELHPNELEVDWERFELTDRAAGLWRRLFPLVEEDTLVEAHVPYRAWLDAAA
                     GSDRQLGWLLRRIAALPATEPEKADLYRSLDLAVRWNLGNSEATRTRTRLPASEVFYH
                     DEPLLRRSGVSLAAELDGPPLTLERLSPAHGRAVLDTALRTSAVRYRELHGFTYGDAK
                     RVWKANIGRGVAVYVCGVPRERRLPLRAYHAATMFKNGVPVGYFETLTLFGRLEVGFN
                     LYYTFREGETAWLFGQLLRLFRQMLGVTCFSVDPYQIGDENDEAIESGAFWFYRKLGF
                     RSTEAPIENLAKAEERKIAAAPGYRTPARTLRRLATAPVIYEMPGGETGAWDRFRVRN
                     IGLALQRSRRREEAPGGLAAILPLIPDFGRWSAEEKRRASDVVRAKSAPDETTYVRTM
                     QRHAKLRDAVLRLGTTAPPK"
     gene            complement(19167..20657)
                     /gene="cobA"
                     /locus_tag="VLE22_12555"
     CDS             complement(19167..20657)
                     /gene="cobA"
                     /locus_tag="VLE22_12555"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HSB15282.1"
                     /translation="MTAARVFLVGAGPGDPELITLKGRRVLESADVILYDHLAPVSLL
                     DFAPPHAERIYVGKKKAIHEFSQAEICAMLVKHALRGLTVVRLKGGDPYIFGRGGEEA
                     EALADAGIPFEVVPGVPSPLGIAAYTGVPLTHRDHTSVVTFVTGHAISQIDWDKVGAA
                     ETLVIFMGLTNFPQIAAEIIARGRPPETPAMAVRWGTRPDQETLAGTLASLPGLIAQR
                     GMKPPATIIVGEVVRLRDKLNWYERLPLFGSRVIVTRARGQAGDLSARLHSLGADVIE
                     FPTIEIRPPVDWSPMDSAIANLSAYDWLIFTSANGVRYFVERLEGSSADLRSLRARIC
                     TIGPATRRAVESLHLKVDLMPEEYVAESLLKAFEPHEIAGKRMLLPRAAVARDLVPAE
                     LARRGARVDVVEAYRTAIPENAAARARAIFSSGRKPDWVTFTSSSTVKNFLETAGRDV
                     LDGVQIASIGPVTSATIRKHGLEVTAEASPFTVDGLVEAVLAHSRH"
     gene            complement(20654..21577)
                     /gene="hemC"
                     /locus_tag="VLE22_12560"
     CDS             complement(20654..21577)
                     /gene="hemC"
                     /locus_tag="VLE22_12560"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HSB15283.1"
                     /translation="MLTIGSRGSQLALWQARWIEGRLEAFGERARIEIIKTTGDKITD
                     VPLAKVGTKGLFTKEIEEALLDRRIDVAVHSLKDLPTEMPEGLVIAAVPERQDPCDAL
                     VGRKLADLPPGAKVGTSSLRRVAQIRALRPDIVIESVRGNVDTRLRKLDEGMYDAIVL
                     AAAGLKRLGWESRIAEILPPEVMCPAVGQGALAVETRGDGGTGQQVCARLNHETTRIA
                     VAAERALLGALGGGCQVPIGAYATCEGETLRLLGLVASPDGETVIRRQTVGTRSDAEC
                     LGRELGRELLAAGGSQILEAVYGFSLRDLKK"
     gene            complement(21581..22846)
                     /gene="hemA"
                     /locus_tag="VLE22_12565"
     CDS             complement(21581..22846)
                     /gene="hemA"
                     /locus_tag="VLE22_12565"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSB15284.1"
                     /translation="MKLTVTGINHKTAPVEVRERLAFDDQALPGALSALRSRPGVAEA
                     LVLSTCNRVEVALACDGEADARACVLDFLTTSRHVPADSVALHLYHHEETEAIQHVFR
                     VASSLDSMVVGEPQILGQLKSAYAAAKAHGALGGYLDAVLTRAFSVAKRVRSETEIGK
                     SAVSVSFVAIELAREIFGSLAGRKVLLVGAGKMSELAARYLHRSGATQIFVTNRTHDR
                     AVEMARLFQGLTVEYDRFVAKLPEIDIVITSSGAPHYLLRKEEMRRVIEARRNRPMFL
                     IDIAVPRNIDPAVNDLDNVYLYDIDDLQKVVDSNRRERLKHAEEAEAIISQEVERTIS
                     RLRAREVTPTIVGLQERLEQVRLAEIERVRGRLGALTPQQEEAVETLTRGLINKIAHT
                     PIAELRRHASLPDGLQTIDMIRRLFRLDD"
     gene            complement(22843..23658)
                     /gene="ccsA"
                     /locus_tag="VLE22_12570"
     CDS             complement(22843..23658)
                     /gene="ccsA"
                     /locus_tag="VLE22_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSB15285.1"
                     /translation="MSTVWLRVAAALYSLGLLQAILAILRRRSQQIFRPALVAFLVAV
                     VLHMVAIVEEGIYTGHFPVNNFWESVTLCAFLLALFFLFVYWRYQFESLSVFVFPLVF
                     VMTLVGSFGRPVASWTNPAVRDVWLLVHVVLVLLGYAAMVLMAAACVIYLIQERQLKS
                     KSPRVLADRLPPLGTLDEFISRAMAIGFVLITLAVIAGSTWAFVESGTHWIREPKIVI
                     SLVTWAFYLAMIFLRVGAGWRGRKAAFLALAVVGFSALTWVAHSNLRVMLGRG"
     gene            23847..24563
                     /locus_tag="VLE22_12575"
     CDS             23847..24563
                     /locus_tag="VLE22_12575"
                     /inference="COORDINATES: protein motif:HMM:NF005594.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB15286.1"
                     /translation="MTNVLGGKKAVVTGGTRGIGRAIAERLLREGADVAICGRSAAGV
                     EQSVAEMSHETGGTVVGTTADVADLNQVRALFTFVDQQLGGLDILVNNAGIGVFASVS
                     RLSSDDWRRTLDTNLTGVYYCCSEALPRFRNRGGGFVINISSLAGKNAFAGGAAYNAS
                     KFGLNGFSEAMMLDHRYDNVRVCTVMPGSVATEFSSRSQNDDWKIAAEDIAEIVACVL
                     RMPGRTLISRVEVRPSKPKK"
     gene            24639..25808
                     /locus_tag="VLE22_12580"
     CDS             24639..25808
                     /locus_tag="VLE22_12580"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB15287.1"
                     /translation="MSIPLDPSRTGREAETLEGHLRKLIVGQEEAVQEIVNVYQTHLA
                     GMTSPDRPIGNFLFLGPTGSGKTRIVEATAECLVGTPRSVIKIDCAEFQHSHEIAKLI
                     GSPPGYLGHRETHPLLSQEMINQWHTDKVKLSFVLFDEIEKASDALWNLLLGILDKAT
                     LTLGDNRRVDFSRAMIFMTSNLGANQMSSILKPNLGFAASETAREHASGHLDDKTADK
                     IMHAGMEAARRKFTPEFMNRLDKVVVFKPLGETELRRILDIELSRLQQRIFDSVGPNY
                     FVFRASDGAKEHLLREGTDVKYGARHLRRAIERLVVQPMSNLIATEQIRAGDLIAIDH
                     AGPENRLVFNREAEGIAISAMAEMTGAKVAVPAIVASEVPAELVKTVGARSSRRA"
     gene            complement(25817..27601)
                     /locus_tag="VLE22_12585"
     CDS             complement(25817..27601)
                     /locus_tag="VLE22_12585"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HSB15288.1"
                     /translation="MTTVLEPTAGAIEAQYDETVRRDIELFREQAEAFLAGNLTDDQF
                     RAFRLRRGIYGQRQPGVQMVRTKIPGGALTAVQMEQLARVADKFAGRKAHLTTRQNVQ
                     FHFVPLRDVPPLLHMLADVRLTTREACYNTVRNVTCCPMAGIARDEVFDVRPHARRIA
                     YAFLHRELTDSLPRKFKIALSGCASDCMAGAIHDLGLRAVVRHENGVEKRGFRMVVGG
                     GLGPLPREAQLLDEFVPEERIVNKCEAVIRVFNTHGNRKNKNIARLKFVVRERGFDWV
                     KEMVEKEYADILANGGIPTPAVIPEGFGGFEPSPPEPASGARLPVIGDHDADDAAYRR
                     WLETNVAEQKQDGYFAVTVRADQGNLTSGQLRAVASVARDAGDGLIRITIDQNMVLAF
                     VRRANLRRTHAALTSAGLGASGAGEIDDVTTCPGAYSCNLGLTKSMNLGHALHQAVRE
                     YDDPAIRRLSIKVSGCPNSCGQHWIADFGFYGNSRKIEGREVPYYQMLLGGGYDRDGM
                     VRFGLGVQSIAARLAPAAVRRVLDHYAANRQDRESFRDYVLRFKVEFFREMTADLAKP
                     PELLPDMYKDWGDDADFSLSLGRGECAA"
     gene            27709..28560
                     /locus_tag="VLE22_12590"
     CDS             27709..28560
                     /locus_tag="VLE22_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15289.1"
                     /translation="MFFTEKRNCSIFFLAIFVILLATSCSTGPPAPKMGTPPFYWGAA
                     GETFAKADYLKTADHLEQLVKTDNEYTQRALPWRLVLTAGMAKSQAELADIFEAGSRA
                     NRGNPTPFRRQMTELRTMAGRRALQFAETFLKFSGHDHTQDIPLDFVFPTGNANKPLE
                     LEKIGNGVPPSETQVESVRRRSIERGVLLATCAAVGAPEDVAKAQSIFKAGNVKVPHD
                     VFALAMVKYLQDQAQLFSRTKLDQPERLELFCNQALAALKTVKPSKETKDLTAKIEKT
                     LKSAKPK"
     gene            complement(28593..30029)
                     /locus_tag="VLE22_12595"
     CDS             complement(28593..30029)
                     /locus_tag="VLE22_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB15290.1"
                     /translation="MSSGTWKWWLAVPAIYFLFFFALTDIGLIGPDEPRYASIGRDMA
                     LSGDWVTPRLWGELWFEKPPLLYWMIGGAHRAGLRGELAARLPVVLLSLGFLIFYYRF
                     LRTEFGPRPALFATVILGSSAGWLAYSQLAVTDVPLAVTFSAAMLLGYRALERGSRRM
                     LAVAAGLLAAAVLAKGLVPLVLALPLLWAWRGRLRRFLDPAPVLVFLVIAVPWFAVLA
                     WKTGFPFVEELFLRHHFARFSSGHQLHARPFWFYVPVLLAGFVPWTPAFVLLLRRGLF
                     SDRRRRFLLIWVAFGFLFFSVSAGKLPGYVLPLFPAVAALIGIAVDEARDARWVLAAA
                     CATLALVPVITGTLPGALATGLSRARIEGWHWWFAITYCLMAAVIWWWEEAGRRQAAV
                     GLLIFATVLGVVFIKVKAYPAIDRLATARPLWRHVSTRAYLTCVEPIHRSLRYGLNYY
                     SGKPLPDCSQLVRPFRVQQLPGHPPFVN"
     gene            30051..30596
                     /locus_tag="VLE22_12600"
     CDS             30051..30596
                     /locus_tag="VLE22_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15291.1"
                     /translation="MFRLGAISLFLLPMAARADVAGCACDPADSASLEARECGLTREA
                     LKQPEAPAVFFLKDINPRKPNRWLALPRQVTKGVYRLPEMTAKERAALWTAAIARAKE
                     LWGEGWGLAMNGDLVRTQCQMHVHLGKFVEGAESDNFVVIRSVEEIPAPDGEGLWVHP
                     TRDGKMHVHTGEILTETILVR"
     gene            30604..32142
                     /locus_tag="VLE22_12605"
     CDS             30604..32142
                     /locus_tag="VLE22_12605"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HSB15292.1"
                     /translation="MSLLDISKAPTAENAVILLHPSDNVAVARTALQGGQEVIAGGRR
                     ITLREPVPAGHKVAVSRIGAGALVQRYGQNIGPASRTIEPGEHVHTHNLLYEERSIDY
                     EFPKAEIPRPVAPAGGPAFHGYLREDGRAGTRNYIAVVAASNCAAHTVELIARSYESE
                     KLPSNVDGVVAFPHGDGCGHAIGPDTILLQRTLSGVLDHPNVSAAVVLGLGCEVNQID
                     HYLGGEAARSGRLAGITLQESGGTRAAVEAARREIAKSIERAESERRVELPASKIVLG
                     LNCGGSDSFSGITANPALGFCSDLLAGAGGTPVLAETPEIFGAEHLLVRRARNRAVAE
                     RVLAFIRSYQEYLRRFGESFDDNPSPGNKEGGLSNILEKSLGAVAKGGTSNLMDAIDY
                     AERIVPGGLVFMNTPGYDPVSLAGLAAGGASVIAFTSGRGSAIGFPSIPVIKIASNSH
                     TFRRMRDNMDVNAGRITDGDAAIEDVGQEIFDLLLRVASGERTASERWGHKEFVPWRI
                     GPVM"
     gene            32160..33098
                     /locus_tag="VLE22_12610"
     CDS             32160..33098
                     /locus_tag="VLE22_12610"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSB15293.1"
                     /translation="MRGEIKALMLALALAALAAAQGTKAAAAKPAGTAFDKVKFEAYV
                     RHLFVWGPHVAVNVGDPKPSELPGFREVVVTGAAGPTSFNQSFFVSADGRKVVQGRVF
                     DIQENPFREELAKLRTGMGPSLGTPGAPVVLVLFTDYQCPFCRQEAQMLRQNLISTYP
                     KEVRLYLKDFPLEPIHPWAKPAAIAGRCIYGQNEPAFWQYHDWVFDQQQTFTPENLRS
                     KVMDWAQGKEIDTLQLGRCIEAKATEADVDNSIAEGRSLQLNATPTLFVNGRRLTNAS
                     WEQLKAIIDFELAYQKTAKNAGDNACCEVKLPSPLQ"
     gene            33104..34636
                     /locus_tag="VLE22_12615"
     CDS             33104..34636
                     /locus_tag="VLE22_12615"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSB15294.1"
                     /translation="MKTRLGRARLLFAVVLSLRVACGVEIDPGQYLEHIRFLASEEMK
                     GRGTGTPELERAADYLAAQFRGFGVEPGNGQSYFQSFPVTAHAHLGKNNQLVWRNGRR
                     QALKFDSDFRPLNFSESGTVSGKVVFAGYGITAPEYNYDDYDGIDVRNKIVLLLRHEP
                     QEFDEKSVFGGRVYTIHAQFESKALNAKSHGARGVIFINDRPNHPSESDDLEQFSGAL
                     GPASPGIPFVQVKAEVAEAWMTRAGRPLADAVKAIDKDLRPQPFALPDSFEVEMRVDI
                     ERETRMVRNVMAYMPGQTGEYLIIGAHYDHIGLGEQYSMASSEAGKKPHPGADDNASG
                     TAGVLELARWFSKQPRGRRGILFVTFAAEEIGLLGSSYFTNNPPRPLGNAVAMINLDM
                     IGRPREGKVYVGGVGTGTTFGSIVNGMNGNGASKLNIDTTTADGYGSSDHFSFTPKQI
                     PVLFFFSGMHGDYHRPSDTWDKIDAPAAAELLEYAGRVAEQLLAAPDRPRFIGVAHAS
                     GH"
     gene            34673..35287
                     /locus_tag="VLE22_12620"
     CDS             34673..35287
                     /locus_tag="VLE22_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15295.1"
                     /translation="MAQSTFESARCWKLPPLILHPFAEASGPDRLTASSRASLILQGL
                     LPGEEFNLEELNRWILEGRFYEVRMLFYIGKDLNRWIGQSMEVVEREDALREGGVRRE
                     SFAAMLVEDPPSAVQEKLRSWGVSDHKAIFRRALGLNAVFADVPLREMLTDEFVRHHF
                     RYADRMFECRQQSARFTPVHSENFPFEMYASGEYARMLEREWEV"
     gene            35330..36604
                     /locus_tag="VLE22_12625"
     CDS             35330..36604
                     /locus_tag="VLE22_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15296.1"
                     /translation="MEFSRLVRVRQRFPDRSIKDIPAEVQRQLAGAAFGAHLKPGSKV
                     AIGVGSRGIASIGTIVGAVVEYWKSLGMQPFIFPAMGTHGAATAEGQAAVLAKYGIHD
                     ASMGCPIVSSLDVVALGVTPEGIETFMDRHAWESDGVMLVGRIKWHTDFIGKIESGLF
                     KMMAIGLGKFAGAERYHTHAYRMGIEAMVRSVGRQVLKSGKILGGLAVLEDANHNAAQ
                     LTAVPAADMERQEEELLALAKSWMGRIPVEALDVLIVDEMGKDVSGAGMDTKVINRNA
                     FANYNPWPNAPIIERLFVRDLSPLTYGNAVGVGMADVVTDRLVEKIDWQPTLINALTA
                     ATLAAARTPIHLPTDRQCLETLARAVGKFDSREVTIGWIRNTLELRELALSENLLPEI
                     EADPALEVLGPPREIEFDAEGNLLPVTEAVHA"
     gene            complement(36613..38046)
                     /locus_tag="VLE22_12630"
     CDS             complement(36613..38046)
                     /locus_tag="VLE22_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15297.1"
                     /translation="MWHRPVILLVIFLFPATWAAAEDRFLAGWASASITPDQPVALEG
                     QVRTRVSTHVQDPVTATALAMERKPETGAVESAILISCDLVAIRTGILEGLRERLASR
                     LSGFDVRKVFLHATHTHTAPAMTPGLYDIPEGVMQPADYIEFLVGRLADCAEQAWKSR
                     KPAGVSWALGHAVVGHNRRAVYKGGDARMYGKTDLPGFSHVEGYEDHSVDLLFFWDHD
                     AKLTGVGINVSCPSQSVEGASYVSADFWHDVRVELRKRYSKDLFVYPMTGASGDQSPH
                     LLLHKRAEQKNLERRGLAARQETARRIVSAVTDAFEIANRDIRFNPPFVHKVEDLRLP
                     VRKVSAAEAENARQEMERTARDAAADRTYVLYRNKGVLERYERQDREPFYAMELHVLR
                     LGDVAIATNPFELYLDFGLQIEARSPAEQTFIVQLAGRGMYVPTARALSGGGYGTEAY
                     VSLVGPEGGEVLVERTVETINALWPER"
     gene            complement(38198..38374)
                     /locus_tag="VLE22_12635"
     CDS             complement(38198..38374)
                     /locus_tag="VLE22_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15298.1"
                     /translation="MKKWFRNFWNDTRGQDLIEYALMAGFVAVAAGAIMPSVSTSIST
                     IFSKIGSVLVAGGG"
     gene            complement(38556..39743)
                     /locus_tag="VLE22_12640"
     CDS             complement(38556..39743)
                     /locus_tag="VLE22_12640"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HSB15299.1"
                     /translation="MSNPLSFLTDQLNEWHAAGTYQALRQLETACMPVSRFDGREVIN
                     LASNNYLGLTNHPNLIEAAVDATRRLGVGSGAVRTISGTMAIHMELERRIAEFKHVEA
                     CVVFQSGFTANAGAVSAILGPEDHIISDELNHASIIDGCRLSRAKIHVFPHRDVATAD
                     AKLAALTGQPGRKLLITDGVFSMDGDIAPLPALVELAEKHGAIMMIDDAHSSGVLGRN
                     GRGTVDHFGLHGRVHIQVGTLSKAIGALGGYVCGSRDFIDYLYQRARPFLFSTSHPPA
                     VAASCIAAFELLEQEPERIENLWSNTRYFKEALKAAGFDTGVSETPITAIMIGDAAKA
                     HAYSRAIFEEGVFATGIGFPTVPRGKARVRTIVTATHTRVQLNRAVEILVRVAKRMEI
                     LPQ"
     gene            40023..40550
                     /locus_tag="VLE22_12645"
     CDS             40023..40550
                     /locus_tag="VLE22_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15300.1"
                     /translation="MSNVYGPRLVTPPVSGHPFCASTEAASEKKTWLGLRRKIFTGVI
                     ARDGQGRLRTEFGVRGQPLVIGIVDPVSLKAYALDPRRETFFELPECPPQPVLPALQK
                     PPGAETQRIEGLECFRRVIPELIEEAWISSELQHVVLERAVDGGVRFTWRMYDIRLGE
                     PDPALFRIPPTYKQA"
     gene            40616..41749
                     /locus_tag="VLE22_12650"
     CDS             40616..41749
                     /locus_tag="VLE22_12650"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB15301.1"
                     /translation="MRRVDFVFFDAGGGHRAAATALKHIVEQEQRPWDVQLMNLQEVL
                     HPLDLFRKLTGLRLQDIYNLMLKKGWTLGSAQLVRPMQWLIRLYRPKQVPLLEAHWRE
                     RQPDMVVSFVPHFNRALKEGMLRARPEGRFVTVLTDLADYPPHFWIERQEQNLICGSD
                     RAVEQARALGHTDERIFRVSGMILNPRFYDPVIVDRRAERARLGLDPEKPTGLVLFGG
                     QGSWVMREIARKLDRSGLDLQLILICGKNEKLARALREYKPRIPTFVEGFTSEVPYYM
                     HLSDFFIGKPGPGSISEALAMKLPVIVERNAWTLPQERYNTEWVLEHRVGRVVPNFRN
                     IDIVAAEMLAPGELARYRANASAIDNRAVFEIPAILQRILEYS"
     gene            41746..>43532
                     /locus_tag="VLE22_12655"
     CDS             41746..>43532
                     /locus_tag="VLE22_12655"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSB15302.1"
                     /translation="MKYRLLSVTLMGGAMLAAQSPAPTPESRLLGWMDRIAQEQLDRR
                     AAEIAKVRTPEQADARKQYVRAKILELLGGLPDYSGPLNANVTGRIDKGKYVIENVVF
                     ESLPKVWVTANLYRPAQAGRYPAVLLPLGHWDQGKPAVQLIAANLALKGFVVLVYDPI
                     GQGERLQAYDQRTGKSLAGGSTDQHFLPGAQSILAGESFARYRIWDAKRALDYLVSRS
                     EVDSERIGCTGCSGGGTLTTYVSALDPRIKVAAPACYMNSFRILFPGPVGDSEQSLPN
                     FLSSGLDQTDYVELFAPKPWLIANTVDDFFPLEGARQVYQEAKRWYSVYGAEERIAWV
                     IGPGGHGTPLPVREAIYGWMIRWLKDGRGDAREEEVEFLSDHELLATKTGQVSTGLGS
                     RDIYEAIRDGAKSRMQSFSAGELLNTLRNWMRPVAETPLNVKVVAETLTADITTQTVI
                     FETERGLEIGGALIIPRGAVRRPGVVVVETGPPPSPLALQLARKGAVVLALSPRGLPW
                     NVSIRQLPGDWLAATRAWLIGKNMPGLRAYDIKRGVDLLAARADVDPASIRAVARDAA
                     GVWLLMAAATDARIGGVWLDRTPHSLRAV"
BASE COUNT         8538 a        13440 c        13564 g         7990 t
ORIGIN      
        1 cggaactccg cggccagcac ccttgggtct tttgtccgcg cgttacgcga gaccctgagg
       61 aaacacgcgt cgctgaatcc cgccgcccgg aactcgtcga ggaaggcgcc gccctctttt
      121 gcaccggcga ttcaggcaaa ccaatcggcc gcggtcatca cctgctctgc cggcaagggc
      181 tgcgacagga tcaactcggc cccgaacact gccgcgcccg gccgcaatac gcgtgcaagc
      241 tcttgcaaaa tggccttcct ggccggattc agattgaaga ttccgttggc aagcgcaacg
      301 tctactgtgt tgtcgcgaat cgggagccgc tcggcatccg cctcgcaaac aagacagttg
      361 cggaagcccg cggccgctgc gctgcgacgt gccctgccgc tcatctcctc gctgaattcg
      421 atcccaatca cccttcccgc gggccctgtg cggcctgcag caacgactga atcgagcccc
      481 gcgccgcacc tcaagtccag aacaacgctg cctctgggaa tctcggcgaa taacgacaca
      541 ttggagacac ccgagaacgc ttccacgctt tcggccggaa gtggtactaa caattcaggg
      601 ggatatccga ggctttcggc aaagcttcgc cccacgggaa acggatgcat agccagcgga
      661 tcccgggccg cggccgagta ggcgtcacgg accttctcac gaatcccctt ttcgttgaca
      721 ggcgtcaacg cgccttcctt ccctcgggag taccggctcg ccacgtaccc gttcaggatc
      781 tcaatgaact cggccacgat accgtcgccg cgcagggtcg tgtgcagccg gccgtccacg
      841 taaaccgagg ctttcggctc ctccctggcg actcagccta cagtcggtag aaagcctcag
      901 ctcctcttta catccagacg ataccgcaac tcttccagat gcgccagggg cgcggccgat
      961 ccgaggtgcc tcttgttgga tctgaccagc atttccagcg tgggagacga gcccgtcgat
     1021 ccggcccatc gatttctttc gacggcctgg tgctggtaga gctcggaggc tatgcgccac
     1081 caccattcag gcggtggcga agctctgcca gtcgctcaat gtctacacga agctgctgcg
     1141 tttgctgccc agccgaggca attagcttct cgatcgttcc gatcagcccg ccgagcctac
     1201 ggatggattc cttttcgagt gccaacagcg aggcgttcac gatgtcctcc cattgggatg
     1261 cgagtcgtga caggttagta aacacaaggt cctcaacctt gtggtgatag tgcttcaaca
     1321 aggtgcctct gacgagcgcc atcggtacca accaagagag gagctcccaa ttacggtcaa
     1381 agatcttgcc cacgcgcaca tcgggcgatc tggggtcttc cgtttgcagt tccatctccg
     1441 tggttcggag tggaacaccg agtgtctcaa ccatccgctc ggaaagccgg ttacggaagt
     1501 cctacagcga ttgcgagagc tgccggctga ctcgccgcac tggttcgacg aactcgttcc
     1561 ggcgcttcct ggatggctcc gccagatcgc tggtccagac cacacctagc cagtcgtcga
     1621 acttctcggt cgcgatacgc tgccacgcag taccatctga gccgctgttt cggtattctc
     1681 cgccaggatc gccggaatgg cctgtggagt tggcacaggt cgatgcggac gtcctgttgg
     1741 tgggccacac tcatctaccc ttccaggcgg tccttgatgg gcgtcaagtc gtcaacccag
     1801 gcagtgtcgg gcagccaaag cacggcttcc cacacgcctg ctgcgccatc tgggaggaag
     1861 gacggttttc tgcgaaatca cggccgtacc tcactgaagt gactgccaga aaagtgctct
     1921 cgcttccgat caaagaacct attgcaggcc aattggccga tgtcttgatg cgtggggtat
     1981 cgccagcctg aatcgccgcc catgatgtta ccgactgtgg ctgctctcac atgggctata
     2041 ttggaatgtc tgtagattga ccggcgatgg agtctcgccg ctaaccgtcc cgggagttcg
     2101 ggaatgatag ctcctgccaa ctggtaggag cttttgcagt tttgggccca acgacgacga
     2161 ggagcttgac cagtgttcaa gaatctcctg accgcggcca gcttgctggc agcaaccgtt
     2221 acgtgcgcac ccgcgcagca gcaacccccg caggccctga cgcttcagga ctgcatccgg
     2281 ctggcggagt cggtcccgtc gtcggtgagt gtcgccctcc aggagcgtga tatcgcggcg
     2341 cgagatgtca cgcaagcccg agccggcttt ctcccccagt cccggttgat caacggcttc
     2401 acctacaata gcccgctgat gaacaaccgc gagactttca gcttcattcc tctcaacggg
     2461 atccgggagt acgcgacgct gttcagtgtg aaccaggaat tcgacacttc gggccgcttg
     2521 cgcgctgaca tgacccgggc acgcgtcgcg caagagatcg cggcgaccag cgtggccctc
     2581 acgcaacgcg acctccggcg cgccgtgacg accgcgtatt atcgggccct cctcacgcgc
     2641 cggatcgtcc ggatcattga ggatgccctc gccgagagtc ggagtttcga gagtagagcg
     2701 aaacttctct tccaaggtgg agaggctgct caggcagatg tggtcaaggc ttcagcgcag
     2761 gtcgccttcc ttcagcaggc tgtcaacgcc gccgaactgg aggccaatct ggccagccag
     2821 gagttagcat cattctggac ccgcgatgtt actggcagcc tagccctggc cgatctcttc
     2881 gatgagagcc ctccgccgcc agagcccgaa gccgtgcaag cggcgccatt tctcaggaga
     2941 ctggagttca gtctcttcga cgcgcagagg cgggggttcc aggctgacgt gcggcgcgcc
     3001 cgttccgccc tgcttcctca acttgggttc acattccaat acggcatcga ctccaccgct
     3061 gtgcggatta gcgatcgcgg ctatgccgca tttgtcaatt tgacgattcc tgtcttcgac
     3121 tggaacaggg ctcgcagcac catgcagcaa gctcggctgc gaacgcaaca gatcgaatca
     3181 acccgcgcaa tttccgagcg gaccttctcg aaggactacc agggcgccct tgcccgggtg
     3241 aagcaactct tcgcgcagat ttcgctcaca agccaacagg cgaagctggc ggaggacgat
     3301 ctgcgtttga gccgcatccg ctatgaaggc ggtgagggat cggcgcttga cgttgtgact
     3361 gcgcagaatc aactcgcgca ggcacggagt aattactacg cgactatggc caactacttg
     3421 aatgccaaag ccgacctgga ggtcgcagca ggacgatgaa accgcaaaac gcgctgctcc
     3481 ttagcctctt cgtcctcgta ggctgcggat cacatgaaga cctgcctcca aagccggttg
     3541 tggcagtggt ggtcgccaaa gccgacgtag gcgacgtcaa atcgatggtg cgagccccag
     3601 ccttcgtgtt cgctcgcgag caagccaaca tcggttcccg tgtgacggcg ccaattcggg
     3661 agttgttagt ccggaagggc gacagcgtcg gagccggcca agtgctggcg caactcgata
     3721 accgagacct gctggcgcaa cgcgatgaag ccgcggcggc tgtaaccgaa gccgaagcca
     3781 acctggagcg cgtgacctcc ggaacactac cgactgatat tgagcgggcc cgtggacagt
     3841 cggccacgac ggaagctgcg ctcaaccaag cccagaaatt ctacgatcgc cggcggcaac
     3901 tttttgagca gggcgccatt ccacagcgag acttgctgct cagtgaaact gagttggcgc
     3961 aggcaaaggc aaaccacgag gtcgccacga gggctctcga cttgcttcag aaccagtccc
     4021 gtgacaagga catcctgatg gccaagagca aggttgagca ggctcgagcg cacctggccg
     4081 cgatcaaggc tcaattgggc ttcaccgaaa tccgaagcac ttctcccgga acgatcaccg
     4141 agcaatttat gtttccaggt gacatggcca aacctgacgc accaatcttc actgtcatgg
     4201 acttggctgt ggcggtggca cgcgcacaga tcccggattc cgaggtagcg gctgtccgaa
     4261 caggtcaaac gtgcgcgttc gtgccggcag atgctgccgg aacctccttt gctggccgca
     4321 tttcagtcgt caaccagtcc attgatacgg ccaggagaac cgtcgagtcc tggtgcgaga
     4381 ttccgaaccc taatcgagca ttacgtgccg gtacgtttgg gcaggtttcg atcgtcacgg
     4441 gcatcgtgcc aaagagcgtt gtcgtccccg tcgctgccgt ccagtttgcg gaaggggcca
     4501 gtaagggcgt cgtgatggtg gccggtgata agggtgtggc cgtgaagagg gaggtggaga
     4561 cgggagacgt ctttgagggc aaggtccaga ttcggaacgg tctgaacgcg ggcgaatctg
     4621 tcatcattca gggtgcttac ggattggcgg atggaactca gattcgcgtg caggagggca
     4681 agaagccgtg accggccgcc ttgcccaatt cgtccaggcg aacgcccgtg cgatggtgat
     4741 catcgtcctg gccttcgccc tggcaggggt ggcgttcatg ttccgcctac ccatcgcgat
     4801 cttcccgcag acggactttc ctcgaatcgt catcctggtg gacaacggca tcactccggt
     4861 ggatatccaa atgctgcgag tcacccgccc catcgaggag acgattcgca tagttcccgg
     4921 aatcacgact gtccgttccg tcaccgcgcg cggatctacg gaaatcagcg tcttctttcg
     4981 ctgggacgtc gacatcctga acgcactcca cctggtgcaa ggccgcatct ctcagatcac
     5041 gccttcgctt ccctccacgt gccgtttcta catcaaccgc ctcacgtttt ctatctttcc
     5101 aatggtgggg tttagcatca cgtctccgac gcggtcgcaa gcgaatttat gggaactggc
     5161 gtactacaac atcgctccgc gcctctaccg actgccaggt gtcgccgaga cgcgaatcgt
     5221 cggcggtcgt ccgcccgaat accacgtttt ggtagatccg cagaaactca acagctacgg
     5281 catgcccttg accaaggtga ccgatgcact tcggagcagc aacacgatct cgccctcggg
     5341 catggtccaa gagaactacc acctctactt gactaccgtg accgggctac tgcgtacccg
     5401 tgagcagatc gaagacacag ttgtcgatgt agtgcgcggc acgcctgttg ccgtcaagaa
     5461 tctcgccaat gtggtgcctg gtgagcggcc aatctacaac atcgtgacgg caaacgggcg
     5521 tcctgctgtg ttggtcaacg ttctgcaaca accagacggg aacgcggttc aaatcgcgga
     5581 ttcagtgaac gcggaattgt ctgatatcaa gaaggccctg ccgcccgata ttgagttgtc
     5641 aacctactac gaccagtctg ttctggttcg ggattccatc gctggtgtta ctgaaagcat
     5701 tctcatcggc ctcgggctct ccgtacttgt tcttctcgtc ttcctgaaga gttggcgcac
     5761 gacgatcgtg gcggcggtgg tgattccaat cgctgtactc atcgcgatcg tgttcatgaa
     5821 atttttcaag atgagcttca atttgatgac cctcggaggt ctggcggcct gtatcggcgt
     5881 ggtgatcgac gatgccatcg tcatggtgga gaacatcatt gtgcacctct ctactggcca
     5941 ggcgccgatg gaagcggcga aaagtgccat cacggagctc acaccggcgt tgactggctc
     6001 aaccttgacg ccaattatgg tcttcgctcc gctggtcttc cttgggggga taacaggcgt
     6061 cttctttcgt gctcttgcga tgaccatggt gacggcgctg ctcgcttccc tcttcctggc
     6121 gatcttcttc acgccggtgc tggcggcggt gttcttccgc cgttccaccg gtgccgccgc
     6181 gacggattta gagcaggcag aacaagctgg cgaaggccgc gtcctgcgct ggctcaccgc
     6241 acgatatgaa gccgggctgc actggtccct ctatcatccg cggctgattc tcctgagcac
     6301 tgtggtcgta ctggctggaa tgatcggagt ctactttcgg ctcggcagcg ggttcctgcc
     6361 ggagatggat gagggggcgt tcgtgctgga ctaccgcaca ccagccggca cttcccttca
     6421 ggagacggat cgagtactgc ggcacatcga gcaattcgtc aaagaaacac cggagatcga
     6481 gagctactcc cgccgcacag gggcgcgcct ggctcttgcc atcgcggagc ccaataccgg
     6541 ggatttcctc atcaaactga agaaagaccg gagacgatct cttgaagacg ttacgtcgga
     6601 actgcgtcac aagatcgtct cgtccgaacc cgtcatcgat gtggagtttc cgcacattct
     6661 ggaagacctc atcggcgatc ttgcatggtc gccgcagccc atagaaatca aagtgcgcca
     6721 cgacgacgcc gaaacctaca aggaggtcgc ccgccggatt gagggatggc tggggaaagt
     6781 caaaggggtc gtggatatcg tgaatcagac catagtgatc gggccatctc acaatttccg
     6841 cgttgacccg gagaaagcaa ggttggcggg gttcggtgtg aaggatgtcg cggaccttca
     6901 ggccgcaatg cttgatggag aactggcatc ggacctgatc cggggagatc gactggtcgg
     6961 gatccgcgtc cgatatccgg cggagtatcg ttcctcaacc gacaagctca agtccctgct
     7021 cttgacctca ccaaccggtc aaacggtacc gatgtcgagc attgcgaccg ttgaagtgga
     7081 tgaaggaaca actgaaatac gccgggagaa cctgcggaac atgtcggcgg tgaccgcacg
     7141 cctcgaaaag cgggatctcg gctccgcaat taaagagatt cagcagaggc tcccgaagga
     7201 gatcgcgctc cctcccggca cccatatcga gtatggcgga ctctacgaga tgcagcgcga
     7261 atcattcctt gggctaacgc aggtcctgct gctctcaatc ctcctgatct tcgtcattct
     7321 cgtgttcgag ttccgctcct tttcgcaccc gatcgccata ttggcggcga cgatcctttg
     7381 tggctcgggt gcgctggtgg ccctatgggt aacgaggacg acgctcaaca tctcctcgtt
     7441 catgggcgcc atcatggtcg ttggcatcgt ccacaagaac ggcattctga tgttggactc
     7501 ggaacagtac ttctcggaga agggttatga attacgtgaa gcgatcttcc atgccgggcg
     7561 gcgccggctg cgaccgattc tgatgaccgc actggccacc attttcggca tggccccgct
     7621 ggcggtcggt gtgggctccg gagcccaact ccttcagccg ctggccatcg ccgtgatcgg
     7681 tggcgtggcc gtttcgatgg ttctatcgtt gctcatcacc ccggtgctct ttttccagct
     7741 tcgtcaacgc gggctttaac ccgcgccgtc gctggcggca gctaacttga atttggatag
     7801 gagcgtattt gtgcgtcaat acatgtctcc tgctggtccc gtgcatgaac gttccgaatg
     7861 gcgggaaaca cgccgacaat cccgtggatt ttcaggagct tatgatcgca ccctacaatg
     7921 cgccttcatt cacagaagcg attcgcaagg gtatggagac cttccacgtg ctgaagcggg
     7981 tgttgcgcgc aaagggctac aaacacggcc gtcggcgacg agggtggatt cgccccggac
     8041 ctcaagtcga acgatgaggc ggtcgaagtg atcatggaag cgatcgtgaa agcaggctac
     8101 aaaccgggcg aggacatctc catctgcctc gatcccgcag ccagcgggat gtgggatgac
     8161 ggaatatacg tcttctccaa gttgacaaga aagccatcac ttcccaagaa ctgatcaaga
     8221 tttgggagtt gtgggcgaat cagtatcctg tcgtcttgct ggaagacggc atgggagaaa
     8281 acgattggga ggggtggaga gacctcaccg tgactctcgg atcacgaatc gagctcgttg
     8341 gggatgaagt gttctgcacg aacccgagaa tccttcagga gggaatcgag aacggcatcg
     8401 ctaacgccat cctcatcaag ctcaaccaga tcggctcggt gacggagacc cttgacacga
     8461 ttgaactggc cgggcgaaac agctatcgat gcttcgtgtc tcaccgctcg ggcgagaccg
     8521 aagacacctc cggcgcgtga ggcgccggaa acgtgacagt ctaagagtac cggctcgcca
     8581 cgtatccgtt caggatctcg atgaactcgg ccacgatgcc gtcgccgcgc agggtcgtgt
     8641 gcagccgccc gtccacgtac accggcgctg tcggttcctc gaacgtccca ggcagcgaga
     8701 taccgatgtg cgagtgcttc gactcgcccg gcccgttcac cacgcacccc atcaccgcca
     8761 ccttcagctc ctcgacaccc ggataccggc ccttccacac cggcatctgc tctcgcaggt
     8821 aggattgaat cctgtccgcc atctcctgga agaatgtgct cgtcgtccgc ccgcaacccg
     8881 ggcacgcggt gacctgcggg ctgaagttcc tgaggcccag cgactgcaga atctgctggc
     8941 tcacgatcac ctcttcggac cggtcaccgt tcggcagcgg cgtcagcgag gttcggatgg
     9001 tgtccccgat gccctcctgc aacaacaccg acatcgccgc cgaactcgcc acgatgccct
     9061 tgctgcccag gcccgcttcc gtcagcccga gatgcagggg atagtcgcac tcgccagcca
     9121 tcatccggta gacctcgatc agatcctgga cgccgctcac cttcgcgctc aggatgatcc
     9181 ggtcgtgtcc gaggccatat cgctccgctg cctgcgccga ttcgagcgcg ctctgcacca
     9241 tcgcccgcag catcaccgtg gccgcatcga gcggctccga ctgccgcgag ttctcgtcca
     9301 tcagccgcgt cagcagcacc tggtcaagcg atccccagtt cacgccgatc cgcaccggct
     9361 tggcattttc gatggcgact tcgatcatcg tgcggaaatt gtcatcgtgc ttcttcccga
     9421 tgttgacgtt gccggggttg atccggtatt tggccagggc gcgggcgcag tcggggtatt
     9481 ttttcagcag gatgtggccg ttgtagtgga agtcgccaac aactggtacg gagacccaaa
     9541 gttttgcgag cgactcgacg atcttcggca cggccgcggc cgccgcctcg gtgttcaccg
     9601 tcacccgcac caactccgag ccggctcgcg ccagctccat cacctggttg acggtggacg
     9661 ccacgtcggc cgtgtccgta ttcgtcatcg actgcacgac gatcggatga gatccgccga
     9721 cctgcacgcc gccgacatcg cacaccacgg cttttcgccg cggtcttcct gccatatccc
     9781 ctccagaatc ttcattctac caatgccatc cggggcgccg ttgttgtaga ctgtctgcga
     9841 tccgcggaag acttatcatg actctcaccc gtctctggct taccgtcgtt tctgccgccc
     9901 ttgccgccgt cgccctgatc gttcccccgg gcggcgcgca gaccggcgcg ctcaaggaag
     9961 tcgtaccagg catctggttc cgcgagggcg atctcaagga gctcggccac tgcaacaacg
    10021 tcatcatcga gatgaaggac taccttgtcg tggtcgatgc caactttccg agcggcgccc
    10081 ggctcgtgct cgccgatgcg aagaaggtat cctccaaacc ggtcaagtat gtcttcgaca
    10141 cccaccacca tggcgatcac gcgtacggca atcccgtctg gacgaaaatg ggcgcggtca
    10201 caattgccca cgtcaacgtc gccgaggaac tgaagcgcta tgagcctaag cgatggctcg
    10261 aaacagcgcg gactcgccag gacgtcgccg aattgaacct tcccgagccc gaaccgccgc
    10321 gccagacttt cgataagagc ccgtacgtca tcgaagacgg ccgccggcgg atcgagcttc
    10381 acttcttcgg ttgggcgcac acccgcgggg acggcttcgc ctacctgccg aaagaaaaag
    10441 tcctatgcac cggcgatgca gccgtcaatg gcccctacaa ctacaccggc gatgccagca
    10501 ttctcaactg gccgaacgtg ttgcgagccg cccagaaact cgacgtcgtc cacgtgctgc
    10561 ccggccacgg cgggcttgcg ggcaaagaat tgctcactgg ccaggcacaa ttcttcatcg
    10621 agctccggaa ggcggtcgat gcggccgtga agaagggcaa gaaagtggac gatctggtca
    10681 ccatgaagga cggcaacccg gccgccacga cggttaaact gcccgagtcg gtgaaaacgt
    10741 gggtcggcga ttccctgccc gcccaggtcc gtgacgccta cgtcgagata acgcgaaatt
    10801 catcacgccc ataaaaaaat tcgatcgctt ttcatcggga aatcacatat gctggttagc
    10861 atacggggcg tgatagttct tgacgtgcgc tcccttcggt ccaagcggac tgaaattctc
    10921 tcgccgcaga gttcatctgg tagagaaagc tggacaaagg agaatcagca atgaacggcc
    10981 ctcgtaaccc gatgaaggtt ctgttttccg gactggacgc gcaacttgca aaggagttgg
    11041 gcgctgccct cgttgaggcg ggtggttgca tccgtgttgc ggatcgcgtc accgccgacg
    11101 taatcttctg tgccgccgac ccggtctgct accgtgccat gctcgagatt ctccggcgcg
    11161 aacaacccgg cctgccggta atcgtcgtca gccgccaccc gagggcctcg gagtggatcg
    11221 acgccattga ggccggggtc acggattact gcgccacgcc ttttgaaccc acgcagttgc
    11281 agtggcttct cgagacctct cgccacgccc gccgtgccgc cgcggcctga gccctttagg
    11341 actcgcggta tactggcagc aggagctggt tcgatcttgt ctatccggtt gtttctgcgc
    11401 ctcgcagtgc acacacagat gctgcccgtg ctcgaagcgg gtgaggagac cctcgtcggc
    11461 ggccaggcag tgatggaggg cgtcatgatg cgcgccccgc acagctattg cgtggcggtg
    11521 cgccgtcctg ccggcgacat gatcaaggac gagatgccgg ttccgcgcgt ctcggaaaag
    11581 tacccggcgt tcaaatatcc gatcctacgg ggtgtgggga cgctcggtca ggctatgtgg
    11641 cttggaatca aagctttacg gttctcagcc aacgccgcgc tagaaaacga aaaggctggg
    11701 gagaagaagc aggagttgtc tccctggctg atgacgctga atctggcgtt ttcgatcgct
    11761 tttttcatct ttttgtacaa atttgtgcca cttttgctca cttctgcact cggaaagtgg
    11821 tttccggcgg tcgaagggcg catcgtgttt aacctcaccg acggactgat ccggatcgcc
    11881 atcttcctca ccttcctgtt tctgatttcg ctttggaagg acatccggcg tgtgttcgag
    11941 taccacggcg ccgaacacaa agtcgtgttc aactatgaat ccggcagacc ggtcacagtc
    12001 gagaacgcac agtccttcac gacattccat ccgcgttgcg gcacaagctt tctgctggtt
    12061 gtgatggtga tcgccatctt cgtatatgcc ctggtaccgt acgacagttt cctcatgaaa
    12121 ttcgtggctc gggtggcgct gttgccactc atcgccggat tgagctacga actgatccgt
    12181 ttcgccgcca agcaccgcgg cggcctcatg gccaccctca ccgctccggg actgtggctc
    12241 cagcgcatta ccacccagcc gccgtcgaac gatcaggtgg ccgtcgccat ccacgccctc
    12301 gaaggggcga tggcgctcga aaacgctcag ggcggccagg ccgtcatcgc ttaaccactc
    12361 cccatgcaat acgctcaaaa gctagatgag atcgaggccc gctttgagga gctgacccgc
    12421 cagatggccg atccggctgt catcagcgac tccgagaact accgtaagac caccaaggca
    12481 cactccgaac tggaagaggt cgtcacgaag taccgggagt ggaagcgggc ggagtcgaac
    12541 cttcgcgaag cccgtcccat gctcgaggag tccgaccccg agttgcggac gatggccgaa
    12601 gaggaggtgg ctcgcctcga gccgaaactc gagcggctgg agcaggagct caaggtcctc
    12661 cttctgccca aggacccgaa cgacgacaaa aacgtccttc tggagatccg cgcgggcacc
    12721 ggcggcgacg aagccacgct ctttgccgcc gagatcttcc ggatgtacac gcggtacgcc
    12781 gagtcgcagg gctggaaggt ggaaatcagc tcggtcagtg attcagcggt cggcggagtc
    12841 aaggaagtga tcgcgctggt gaatggcgac aaggtgtaca gcaagctgaa gtacgaaagc
    12901 ggcgtgcacc gtgtgcagcg cgtgccagtg accgagcagc aagggcgcgt acacacctcg
    12961 gcgatcacgg tagcggtgct gccagaagcg gacgaggtcg aggtccagat cgacccgaag
    13021 gatattcgca tcgatacgtt ctgctcatcc ggtccgggcg ggcaatcggt gaatacgacc
    13081 tattcggcgg tgcgcatcac gcacctcccg acggggttga tcgtctcctg ccaggacgaa
    13141 aaatcgcaga tcaagaacag ggctaaggcg atgcgtgtcc tgcgttcgcg gctctatgaa
    13201 atcgaactcg agaagcagca agcccagatc ggcgcggagc ggcgcagcat ggtgggcacg
    13261 ggcgaccgca gcgagaagat ccgcacctac aactttcccc agaaccgcgt caccgaccac
    13321 cgcatcggac tcacactgca ccagctcgac ctgatcatgg acggccgtct ggacgtgatc
    13381 attgacgcgc tgtccacata ctaccaggcc gagaaactga agcagcagac cgcgcccgcc
    13441 ggcgcccccc atgacagttc acaccgcact ccggcagggg tccaggcttc ttgaggaagg
    13501 ccacgtcccg gcgccgcgcc tgacggcgga agtactgctc gccagggccc tcggctgcga
    13561 gcgtggccgc ctctattccc atcccgagga agagctgagc gatctcgcca ggttgcacta
    13621 tggccgctat ttgcacgagc ggttgcgagg caagccgacc cagtacatca cgaaacgcca
    13681 ggagttctac ggacgcgagt ttgaggtcac gccggaagtg ctgattcccc gcccggagac
    13741 cgagcacgtg gtggagacgg cgctggcgca ttccagccgg ggacggcggg ttgtggatgt
    13801 gggttgcggg tccggggcaa tcgcggtgac gctgcgactg gagaccggcg cggaggtgtg
    13861 ggggacggac atctcgcagg tggcgctggt ggtggcagcg cggaacgcgt cgcggctcga
    13921 cgctgcggtt catctgatcg tgtgcgatgt attggacgcc gttgcgtcgc gtagcgtgga
    13981 tctggtcgtt tccaacccac cctatgtggc gcttcacgaa gcgccgggcc tccagcgcga
    14041 ggtgcgcgac cacgaaccac acgtggcgct gttcgccggc gagacgggct tcgagattta
    14101 cgagcgcatt gtcgccgacg gcgcgcgcgt gctgaacccg ggtggccgcc ttgtgctcga
    14161 actgggcttt aagtcttgcg accgcgtccg cggtatgctc gcagccggct gggaagacat
    14221 tgaaatcatc cccgacctcg ccggcatccc ccgcgtcatc tccgcccgtt gggcgggtgc
    14281 aaaatagggt gtggtgcgca cgatcttcca cgtcgacatg gacgcgttct tcgtgtcggt
    14341 ggaggaactg ttcgatccgt cgctcaaggg gaagccggtg gtcgtcggcg gccgcccgga
    14401 tcagcgcggg gtggtgtcgg ccgcgtccta cgccgtgcgc aagtacggag tccactcggc
    14461 catgccgcta cggacggcct acaagctttg cccgcacgcc atcttcgtgg atggccaccc
    14521 gcaccgctat cgcgagtact ccgacaaagt cttccgggtg ctcggcagct ttaccccgca
    14581 ggttgagatg gcctcgatcg atgaagccta cctcgacatg actggtacgg aacgcttgta
    14641 cgggcctcct ctccgcgccg ctcatctgct gcatgatcgc gtcagggctg aaacgcaact
    14701 gaattgctcg atcggcatcg ggacgtcgcg gctggtggcg aaaatcgctt ccgaccaggc
    14761 gaaaccgaac ggcgtcctgt gggtgctgcc ggggcaggag gcgcggtgcc tcgccgccct
    14821 cgatgtacga aaaatccccg gcgtcggcaa ggcgaccgaa caacaactgc accgcctcgg
    14881 tatacgcaag gtgggcgatc tggcgaaact cgacgattcc ttcctcgagg agaagttcgg
    14941 taagtggggc ctggcactgg ccggcaagtc acgcggactg gatgccggag gctggttcga
    15001 cggcgtcatc ggcgacgagg aagacccgaa gtcgatcagc cacgagcaca cgttcgacga
    15061 ggacactgcc gacctggagc ggctcgaatc gacgctggcg cgtctctcgg aaatggtggg
    15121 ccggcggctg cgcgagcatg ggctatacgc gcgaaccgta cagctcaagc tgcgctacaa
    15181 ggatttcact accattaccc gcgctcgcac gctcgaccac gccacgcagc tcgacacgga
    15241 gttattcgac gaggcgcgga gcctgttcgc cggtaactgg aagcgcggcg ttccggtgcg
    15301 gctgctcggc gtgcaggcat cgtctttcga ctcgggcgaa ggacagatga acctccttga
    15361 ggcggaaaag aacgaacgct ggcggaaggc gctctcggcc gcggaccgct tgcgcgaccg
    15421 tttcggtgag tccgccgtat cgctcgctac cggcatgaaa ggcggcttcc gcgagcgcgt
    15481 ccacgagaac cccgccggat tgcctggtaa ggagccgaag ggcaaaaaga acccgcgaga
    15541 tgtgtaagaa atcgtcgatt tcatagaaaa aatgcagcga atttcctgat ttttccgatt
    15601 ggcataaaag ttgcttgtgg gttctactgg tgaggaggag gagtgatatg cgttgcttgc
    15661 tggcagtcgc ggcgggcttt ttcttaatcg gaattccgct cgccgcagat cgtgaaaacg
    15721 gacaagggaa cgacgagggc aaccacaggc acaagcacca ctacaagggc cgctacaagg
    15781 actatgacgg tccgtcagcc gtcgcggtga acatcttcgt cggccacgat cagcggatcg
    15841 ttggcgattt tctgatgggc cgcccgggca gtgggcttcc accaggactg gcgaagcgtg
    15901 gcggcgacct gccgcccggc ctcatgaagc agctctatcg gaagggacac cttccgcccg
    15961 gactcgaaaa gaagatctac ccgttcccgg tcgacgtgga gcaacggctc tctccactct
    16021 caccgggcct tgcgcgcggg tacatcggcg gtcgcgcggt gattttcaat agaaagacct
    16081 cggcgatctt cgacgtgttc gtcccgctcg attaggccgg acaccctggt ggttcttacc
    16141 ctgcctccta tttccggatg ctgactgaga tgccgcccgt tggggtccgt gtgatcttcg
    16201 tctcgaggtt gggatcggtc gtgatggcct tgagatagtc gggctggcgg tgttcgaagt
    16261 cgttggcgtt gtgcgccttg atggcaccgc ctggcgtcac ccgcccgtaa tgaagatcga
    16321 ggaaccgctt gttatgcggg gttccggtgt ccatgaagac aaagtcgaag gagccgcgga
    16381 gagatggaat ctccttagaa tgcgtcgttg actcgacact cgaccacgct gtcaagcccc
    16441 gcctcgcgaa agttctcggc cgcggccttc gctcgttcgg cattcatttc gagcgttatc
    16501 aacttgccgc cattcctctt gaacgccatt cccatccaga tggcggagtt tccctcggcg
    16561 gtgccgattt ccaggccctg tttgtagcct ttctcgacga tgagatcgta tagaatgcgg
    16621 ccatcctgcg gagagaaccc gtggagccga tcgagcaccg tccgcgccgc gcctggatcg
    16681 atgccttcaa gagagcggtc gagagaatcc cgcatcatct cgctcatcgc ccgcttgtat
    16741 gcggcggtgt tggaggccag cacaaagcgc gaatcatcga catcaacgtt gtgctggatt
    16801 tccgtgaatt tcatcacctg ctcgcgcccg ccttccagga ctcgcacggt gaatggaacc
    16861 tgaactccgt ccacgaggcg atagtcctcg aacaagaaga tcccgagccg ggagagcagg
    16921 cccgtgccgg cgtcgaagtg agccacaacc gccttgcctt gcgcgggttt gaactcgacc
    16981 acccagactt catggctgcc actcttctcc ctgcttttga ccgtcatgtc gggaaagagc
    17041 tccgtgagac ggaacgggac ctggaagtca aaaccatggt tttctgaaac gagatcgtcc
    17101 ggcttgacgg tctccgtgcc gtccggagtc tgggtccagg ctgatacgcc gtcgcaggcg
    17161 cgctcgatca tcaagacgcc tgggatcgtc atccggtaat cccactttcc cggcgccttg
    17221 ccgtacacct cgacgggaaa gcccggggcg tcgggacggg gatgccacgt ccctctgtgg
    17281 atctgggtag taatgcggcc cgccgcttcc cgtcccccag cggcacggag atgtctctcg
    17341 acgagttgct cggcggacgg atgatcgggg ccggctagag cgggagcggc aagaacgacg
    17401 aacagggcaa gttgacagcg gactatttcg gatctcatag tctcaattac tcagatacgt
    17461 gccggaaccg aaaaggtttc ggcatgcgtt ctttttggta tcttttcagg ggacttccgg
    17521 accatgacca aactcgtgga gaagctggag gaggcgaagc gccgtttcgg accgggcgag
    17581 gcacaacgaa ccgaagcgct cctaaatgaa gcagcgaagg cgcgagttat cgaagcggat
    17641 gagttgatcc ggttccacga agcgctgttg tttcaccgcg cgtatccgaa gagcgaagga
    17701 gtgcttcagc gcgccgagga cctgctggca tcgttcggag agcgtgccgc acgagcggac
    17761 ccggagccgt tcgaggagcc ggaagtgtcg ggcatcgccg gtacggcgtt ttccgcaatc
    17821 ttcagctatg acgtagcacg gcggcttgtt gaacttcatc ctaatgaact ggaggttgac
    17881 tgggaacgat tcgagctgac ggatcgcgct gcggggcttt ggcgccggct gtttccactg
    17941 gtggaggagg acacactggt cgaggcgcac gtgccctacc gggcgtggct ggatgccgca
    18001 gcgggcagcg atcgccaact cggatggctg ctgcgccgca tcgccgcgtt gcctgccacg
    18061 gagccggaga aggccgatct gtatcgaagc ctcgatctgg ccgtgcggtg gaatctcggc
    18121 aattcggagg cgaccaggac gaggacgcgg cttccggcga gtgaggtctt ctatcacgat
    18181 gaacctctgc ttcggcgaag cggcgtgtcc ctggcagcgg aactcgatgg gccgccacta
    18241 acgttggagc ggctttcccc agcccacggg cgggcggtgc tcgatacggc cctccggact
    18301 tccgcggtcc gctaccgcga gctgcatggg ttcacgtacg gagacgcgaa gagagtctgg
    18361 aaagcgaata ttgggcgagg ggtagccgtt tacgtgtgtg gcgtgccgcg ggagcggcgg
    18421 ctgccgttac gcgcctacca cgcagccacg atgttcaaga acggcgtgcc cgttggctac
    18481 ttcgaaaccc tgacgctgtt cggtcgactg gaggtaggct tcaaccttta ctacaccttc
    18541 cgcgaaggcg aaaccgcctg gctgttcggg caattgctgc ggctgttccg gcagatgctc
    18601 ggcgtgacct gcttctcggt cgatccctac cagatcggcg acgaaaacga cgaggcgatt
    18661 gagtcgggcg cgttctggtt ttaccggaag ctgggcttcc gctccaccga ggcgccgatc
    18721 gagaacctgg cgaaagcgga agagcggaaa atcgcggcgg cgccgggata ccggacgccc
    18781 gcgcggaccc ttcggcggct ggccaccgcg ccggtcatct acgaaatgcc aggcggcgaa
    18841 acgggcgcct gggaccgttt ccgcgtacgg aacattggcc tcgccttgca gcggagccgg
    18901 cgaagggagg aagcgcctgg aggtctcgcc gcaatcctgc cactgattcc ggatttcggc
    18961 cgctggagcg cggaagagaa gcgccgtgcg tcggatgtcg tccgtgcaaa gtcagccccc
    19021 gacgagacaa cttatgtgag aacgatgcag cgccacgcga agctgcgcga cgccgtcctg
    19081 cggcttggaa ctaccgcgcc gccaaagtga agccaggaca ttggacggac cgtcacacgc
    19141 agcttgtcag aggcaggggg gatgtgtcag tggcgtgagt gcgccagcac ggcttccacc
    19201 agcccatcga ccgtgaacgg cgacgcctct gccgtaacct ccaggccgtg ctttcgaatc
    19261 gtcgcgctgg tgaccggccc gatcgaggca atttgtacgc cgtccaaaac atcccgtccc
    19321 gcggtctcaa ggaaattctt tacggtcgac gagctcgtga aagtgaccca gtccggctta
    19381 cgtcctgacg agaagatcgc ccgggcgcgg gcggccgcgt tctcagggat cgcggtccgg
    19441 taggcctcaa ccacatccac ccgggcgcct cgccgcgcca gttcggccgg cacgaggtca
    19501 cgggccactg ccgcgcgcgg cagcagcatg cgcttgccgg cgatctcatg cggctcgaag
    19561 gccttcagga ggctttccgc cacgtattcc tccggcatca ggtcaacctt caggtggagc
    19621 gactcgacag cgcggcgcgt ggccggtccg atcgtgcaga tgcgcgcccg caacgagcgc
    19681 aggtccgcgg acgaaccctc aaggcgctcg acaaagtagc gtactccgtt ggcgctagtg
    19741 aagatgagcc agtcataggc ggacaggttt gcaatggcac tgtccatcgg cgaccaatcc
    19801 acgggaggcc ggatctcgat cgtaggaaat tcgatcacat cggcgcccaa cgaatgcaga
    19861 cgggcagaga gatcgccggc ctggccgcgg gcccgtgtga ctatgacccg cgagccgaac
    19921 agcggcagcc gctcatacca attgagcttg tcccgcaggc gcacgacttc gccgacaatg
    19981 atcgtggccg gcggcttcat ccctcgctgc gcgatcaggc cgggcaacga ggccagcgtt
    20041 ccggcaagcg tctcctgatc cggccgcgtt ccccagcgga ccgccatcgc gggcgtttcc
    20101 ggcggccggc cgcgcgcgat gatctcggcg gcgatctggg gaaagttcgt gagccccatg
    20161 aagatcacca gcgtttcggc cgctccgacc ttgtcccaat cgatctggga tatggcgtgg
    20221 ccggtcacaa acgtcaccac cgacgtgtga tcgcgatgag tcagcggcac tcccgtgtag
    20281 gcggcgatcc ccagcggcga tggcacacca ggtaccacct cgaacggaat gcccgcgtct
    20341 gcgagcgcct ccgcttcttc cccgccccgt ccgaagatat acgggtcgcc gcctttcagc
    20401 cggacaacgg tgagcccgcg cagcgcatgc ttgaccagca tcgcgcagat ctcggcctgc
    20461 gaaaattcgt gtatcgcctt tttcttcccg acgtagatcc tctcggcgtg cggtggagcg
    20521 aagtcgagca aagacacggg cgcgagatgg tcgtacagga tcacgtcagc ggactccagg
    20581 acgcgtctcc ctttcagggt gatcagttcc ggatcgccgg gtccggcgcc cacgagaaag
    20641 acgcgggctg cggtcatttc ttgaggtccc gaagcgagaa gccgtagacg gcttcgagaa
    20701 tttgactgcc gccggcggcc aacagttcgc gcccgagttc acggccaagg cattccgcgt
    20761 cggaccgcgt cccgacggtc tgccggcgga tcacggtctc gccgtccggc gacgccacca
    20821 gcccgagaag acggagtgtc tccccctcgc acgtcgcata cgcgccgatg ggaacctggc
    20881 agcctccgcc gagcgcaccc agtagcgcgc gctccgcagc caccgcaatt cgggtcgtct
    20941 cgtggttcag gcgcgcgcac acctgttgtc cggtgcctcc gtcgccgcga gtttcgacgg
    21001 ccagcgcgcc ctgcccgacc gcgggacaca tgacctcggg aggaagaatt tcggcgatgc
    21061 ggctttccca ccccaggcgt ttcaggcccg cggccgcgag cacgatcgca tcgtacatcc
    21121 cttcgtcgag cttgcgaagt cgagtgtcga cattgccgcg gacagattcg atcacgatgt
    21181 ccggccgcag cgcgcggatc tgtgccacac gtcgaaggga gctcgtccca accttcgcgc
    21241 ccggtggaag atccgccagt ttgcggccga ccagtgcgtc acacgggtcc tggcgctcgg
    21301 ggacagcggc aatgaccagg ccctccggca tctcggtagg aaggtccttc aagctgtgta
    21361 ccgccacgtc gatccgtcgg tcgaggaggg cttcctcgat ttccttcgtg aagagaccct
    21421 tggttccgac cttggccaga gggacatcgg tgatcttgtc gccggtcgtt ttgatgatct
    21481 cgatgcgcgc tctctcgccg aacgcttcga ggcggccctc gatccagcgc gcctgccaga
    21541 gtgcgagttg ggaacccctg gagccaatcg tcagcatggg tcagtcgtcc aaccgaaaca
    21601 ggcgccggat catatcgatg gtttggaggc cgtcgggcag tgaagcgtgg cggcgcagct
    21661 cggcaatggg ggtatgcgcg atcttgttga tcaggccgcg cgtcagcgtc tcaacggcct
    21721 cttcctgctg cggagtcagc gcaccgagcc ggccgcgcac tcgctcgatc tccgcgaggc
    21781 gcacctgttc cagccgttcc tgaagcccga caatggtggg cgtcacctcg cgggcccgca
    21841 agcgcgaaat cgtgcgctcc acctcctgcg agatgattgc ctccgcctct tccgcgtgtt
    21901 tcagcctctc cctgcggttc gagtcgacca ccttctgaag atcgtcgatg tcgtaaagat
    21961 agacattgtc caggtcgttg acggcagggt ctatgttgcg cggcacggcg atgtcgatca
    22021 gaaacatcgg ccggttccgc cttgcttcga taacgcgccg catctcttct ttgcgaagga
    22081 ggtagtgagg cgccccgctg gatgtgatca cgatgtcgat ctcaggcagc ttcgcgacga
    22141 accggtcgta ctccactgtg aggccctgga acaaccgggc catttcgaca gcgcggtcgt
    22201 gggtccggtt ggtgacgaag atctgcgtcg ctccggaacg gtgaagataa cgcgcggcca
    22261 actcggacat cttgcccgcg ccgaccagca gcacctttct tccggcgagc gacccgaaga
    22321 tctcacgggc cagttcgatt gcaacgaagc tgaccgagac ggcgcttttt ccgatctctg
    22381 tctcggagcg gacccgcttg gccacgctga aggcgcgcgt caggaccgcg tcgagatagc
    22441 caccgagagc gccatgcgcc ttggcggcgg catacgccga tttaagctgg ccgaggatct
    22501 gcggctcccc aaccaccatc gagtcgaggc tcgacgccac gcgaaacacg tgctgaatcg
    22561 cctccgtctc ctcgtgatgg tacaagtgaa gcgccacgga atcggcggga acgtggcgcg
    22621 aagtcgtcag gaagtcgagc acgcaggcgc gagcatccgc ctccccgtca caggcaagag
    22681 ccacctcgac gcggttgcag gtggaaagca caagcgcctc agcgaccccg ggccgcgagc
    22741 ggagcgcgga aagcgcgccg ggcagcgcct gatcatcgaa cgccagccgc tcgcgcacct
    22801 cgacgggcgc cgtcttgtga ttgatgcctg taaccgtgag cttcatccgc gtccgagcat
    22861 aaccctcaga ttggaatgcg ccacccaggt gagcgccgag aagcccacca cggccagggc
    22921 gagaaacgcg gccttccggc cgcgccagcc ggcgccgact cgaagaaaaa tcatcgccag
    22981 ataaaacgcc caggtgacaa gtgagatcac tatcttgggc tcgcgaatcc agtgcgttcc
    23041 ggattcgacg aacgcccagg tactccccgc gatcaccgcg agtgtgatca ggacgaatcc
    23101 tatcgccatg gccctcgaga tgaactcgtc caaggtcccg agcggcggca aacggtcggc
    23161 gagcacgcga gggcttttcg acttgagctg gcgctcctgg atcaggtaga ttacgcacgc
    23221 cgccgccatc agcaccatgg cggcataccc gagcagcaca aggaccacgt ggacaagcag
    23281 ccagacgtcc cgaacggccg ggttcgtcca ggaagcgacc ggccggccaa agctaccgac
    23341 gagcgtcatc acaaaaacga gcggaaagac gaaaacactg aggctttcaa actggtaacg
    23401 ccagtagacg aacaggaaga acagcgccag cagaaacgcg cacaatgtca ccgactccca
    23461 gaagttattg accggaaaat ggccggtgta gatgccctcc tcgacaatag caaccatgtg
    23521 gagcaccacg gcgacgagaa aggcgacgag cgctggccgg aagatctgct gcgagcgccg
    23581 gcgaaggatg gctagaatcg cttgcagcaa tccgagcgag tagagcgcgg ccgcaacccg
    23641 cagccagacg gtgctcatct catgcatttc cggaatattc cttccaaaaa gaggaggttg
    23701 aactttcagt atagcggacg accgtcaccg tgaaactggt gcgcagggtg cgtaaaaacc
    23761 agcgtaccgg actacgaaat gttccccgcc cctgtttgcg tgagtcgtca ccccgcaaac
    23821 gcggcggcat ggcccataat cgaaaaatga ccaacgtact gggtggtaag aaagcggttg
    23881 tgaccggcgg aacgcgcggt atcgggcgcg ccatcgcgga acgcctcctc cgggaaggcg
    23941 ccgacgtcgc catctgcggg cgctcggcgg cgggcgtcga acagagtgtc gcagagatgt
    24001 cgcacgagac aggtgggaca gtggttggaa ccaccgccga cgtggctgat ctcaaccagg
    24061 tgcgcgcttt gttcacattt gtggaccagc aactcggcgg tctcgacatc ctggtcaaca
    24121 atgcgggaat cggtgtcttt gcgagcgtaa gtcgattgtc ttcggacgat tggcggcgta
    24181 ctctcgacac gaacttgacg ggtgtttact actgctgctc ggaggcgctt cctcgttttc
    24241 ggaaccgggg aggcggattc gtcatcaata taagtagcct ggctggcaag aacgcattcg
    24301 cgggtggggc tgcatataac gcatccaagt tcgggttgaa cggattcagc gaagcgatga
    24361 tgctggatca ccggtacgac aacgtgcgag tctgcacggt aatgccgggg agtgtagcca
    24421 ccgagttcag ttctcgctca cagaacgacg attggaagat cgctgccgag gacatcgctg
    24481 agattgtggc ctgcgtgctg cggatgccgg gacggacgtt gatcagccgg gttgaggtgc
    24541 ggccatcgaa gccgaagaaa tagttggcaa ttaaattgct ctacatttct tcggagccgg
    24601 ctgtcggcat ccggaggtaa aaagttcaga tggttcaggt gagcattcca ctggatcctt
    24661 cgcgaacggg gcgcgaggcg gaaacgctcg aggggcacct gcggaaactg atcgtgggtc
    24721 aggaagaagc ggtccaggaa atcgttaacg tttatcagac gcacttggcg gggatgacga
    24781 gtccggatcg cccgatcggt aacttcctgt ttctgggacc gacgggatcg ggcaagacga
    24841 ggatcgtcga agcgaccgcg gagtgtctgg tgggaacgcc gcgttcggtt attaagatcg
    24901 actgcgcgga atttcagcat agccacgaga tcgccaagct gattggctcg cctccgggtt
    24961 accttgggca ccgggaaact catcccctgc tgagccagga gatgatcaac cagtggcaca
    25021 cggacaaggt gaagcttagc ttcgtgttgt tcgatgagat cgagaaggcc agcgacgccc
    25081 tttggaacct gctgctcggc atcctcgaca aagcgacgct gacattggga gataaccgcc
    25141 gtgtcgactt ttcgcgggcg atgatcttca tgaccagcaa cctcggtgcg aaccagatga
    25201 gttccatact gaaaccgaat ctgggattcg cggcttccga aacggcacgg gagcacgcct
    25261 ccggccatct ggatgacaag accgccgaca agatcatgca cgccggtatg gaagccgcgc
    25321 ggcgaaagtt caccccggag ttcatgaacc ggctggacaa agtcgtggtc tttaagccgc
    25381 tcggagaaac ggaacttcgg cgcattctcg acatcgagct tagccgattg cagcagcgta
    25441 tttttgactc agttggtcca aattacttcg tctttcgcgc ctccgacggc gccaaggagc
    25501 atctgctgcg ggaagggacg gacgtgaaat atggcgcgcg ccacctgcgg cgggccatcg
    25561 aacggctggt cgtgcagcct atgtccaacc tgatcgctac ggagcagatc cgcgccggcg
    25621 acctgatcgc gatcgaccac gccgggccgg aaaaccgttt ggtcttcaac cgcgaggcgg
    25681 agggtattgc gatctcagcg atggccgaga tgactggcgc caaggttgct gtgcctgcaa
    25741 tcgtcgctag tgaggtaccc gcggagctgg tcaaaaccgt cggcgcgcgc tcctcacgcc
    25801 gcgcctgact ccgcgcttac gccgcgcact cccctcgccc caggctgagc gagaagtcgg
    25861 cgtcgtcgcc ccaatccttg tacatgtccg gcaacagctc gggcggctta gccagatcag
    25921 ccgtcatttc gcggaagaac tccactttga aacgcagaac gtaatcgcgg aacgattcgc
    25981 ggtcttgccg gttggcggcg tagtggtcca acaccctccg taccgccgcc ggcgccaacc
    26041 ttgcggcaat gctctgcacg ccgagaccga accgaaccat cccgtcccgg tcgtatcctc
    26101 cgccaagcag catctggtag tagggaactt ccctgccttc gatcttcctc gaattcccgt
    26161 agaatccgaa gtcggcgatc cagtgctgtc cgcaagagtt tgggcaaccg ctcaccttga
    26221 tcgagagccg gcgaattgcc ggatcatcgt actcccgcac cgcctggtga agcgcgtgac
    26281 cgaggttcat cgacttcgtc agcccgaggt tgcacgagta cgcgcccggg cacgtggtga
    26341 cgtcgtcgat ctcgccagct ccggaggctc cgagcccggc gctcgttagt gcggcgtgag
    26401 tgcggcgcag gttggctcgc cgaacgaagg cgagaaccat attctggtcg atggtgatcc
    26461 ggatcaaacc gtcgccggcg tcgcgggcga cggaggcgac tgcgcgcaac tggccgctgg
    26521 tgagattgcc ctggtcggcg cggacggtga ccgcgaagta gccgtcctgc ttctgttcgg
    26581 cgacgttggt ttcgagccag cgccggtagg ccgcatcgtc cgcatcatgg tcgccgatca
    26641 ccggaagcct ggcgcccgag gctggctccg gcggtgacgg ctcgaagccg ccgaagccct
    26701 cgggaatgac agcgggcgtg gggatgccgc cgttcgccag aatgtccgcg tactccttct
    26761 ccaccatctc cttcacccag tcgaagcccc gctcccgaac cacgaacttc aaccgcgcaa
    26821 tgttcttatt tttgcggttg ccgtgcgtgt tgaacacacg aatcaccgcc tcgcacttgt
    26881 tgacaatccg ctcctcggga acgaattcgt cgagcaattg cgcctctcgg ggcaacggac
    26941 ccagtccccc gccgaccacc atgcggaatc cgcgcttctc cactccattc tcatggcgca
    27001 ccaccgcgcg gagaccgagg tcgtgaatcg ccccggccat gcagtccgag gcgcagccgg
    27061 agagagcgat cttgaacttt ctcggaaggc tgtcggtcaa ctcccggtgc aggaaggcgt
    27121 aggcgatgcg ccgggcgtgc ggacggacgt cgaacacctc gtcgcgggca atgccggcca
    27181 tcgggcagca ggtgacgttg cgcaccgtgt tgtagcatgc ctcgcgcgtc gtgaggcgga
    27241 catcggccag catatgcagc aacggcggga cgtcgcgtag cggcacgaag tggaactgca
    27301 cgttctgccg cgtcgtgagg tgcgcctttc tgcccgcaaa tttgtccgcc acccgagcga
    27361 gctgctccat ctgcacagcg gtgagcgctc cacccggaat ctttgtccta accatttgta
    27421 cgccgggttg tcgctgcccg taaatgccgc gccgcaaccg gaaggcacgg aactggtcgt
    27481 cggtgaggtt tccggcaagg aacgcctcag cctgctcccg aaaaagctcg atgtctcggc
    27541 gaaccgtttc gtcgtactgc gcctctatgg cgcccgcggt cggttccagg acagtcgtca
    27601 tggtatatac cccattctct atagagaata gcagatttta atttccttga caaggaagct
    27661 tgcaaagaag ccaccgattg tgataaagca gacgtgcagg agaacccgat gtttttcact
    27721 gaaaagagaa actgctctat attctttctc gcgatattcg tgatcctgtt ggcgacctcg
    27781 tgttccacgg gtccgcccgc tcccaaaatg ggcacccctc cgttctattg gggtgcggcc
    27841 ggggaaacat tcgccaaggc ggattatcta aagactgccg atcatctcga acaattggtg
    27901 aaaacggaca acgaatacac acaacgcgct cttccgtggc ggttggtgct cacggcgggc
    27961 atggctaagt cccaggcgga actggccgac atcttcgaag ccggctcacg ggcgaaccga
    28021 ggcaacccga ctccgttccg caggcagatg accgaactcc ggaccatggc tggtcgccga
    28081 gcgctgcagt tcgccgagac tttcctgaag ttcagcggac acgatcacac gcaggacatt
    28141 cccctggact tcgtcttccc cacgggcaac gccaacaagc cgcttgagct cgaaaagatc
    28201 ggcaacgggg tgccgccttc cgaaacgcaa gtggagtcgg ttcggcggcg ttcgattgag
    28261 cggggtgtcc tgctggcgac ctgcgcggca gtcggagcac cggaggatgt ggccaaagct
    28321 cagagcatct tcaaggcggg caatgtcaag gttccccatg atgtcttcgc gctcgccatg
    28381 gtgaaatact tgcaggacca ggctcagttg ttcagccgca cgaagcttga ccagccggag
    28441 cggctcgaac tgttctgcaa ccaagctctg gccgctttga aaactgtcaa accttccaaa
    28501 gaaaccaagg acctgacggc gaagatcgag aagacgctca agagcgcgaa accgaagtag
    28561 gccaccggaa tacgggggag gagcgcgccc catcagttga cgaacggcgg gtggccggga
    28621 agctgctgca cgcgaaacgg acgtacaagt tgggagcaat ccggcagtgg cttcccggag
    28681 tagtaattca gcccgtagcg caagctccgg tggatcggtt ccacgcaggt gagataggcg
    28741 cgcgtggaca catggcgcca gagaggacgc gccgtcgcaa gccggtcaat cgccggatac
    28801 gccttcactt tgatgaacac cacaccgagc acggtggcga atatcagcaa tccgaccgct
    28861 gcctggcgcc ggcccgcctc ttcccaccac cagatcaccg ccgccatcag gcagtaggtg
    28921 atcgcaaacc accaatgcca tccctcgatt cgggcgcggg aaaggcccgt agcgagcgcg
    28981 cccggcagcg ttcctgtaat caccggcacg agcgccagcg tggcgcaggc ggcggccaga
    29041 acccaccgtg cgtcccttgc ctcgtcgacc gcaattccga tcagcgccgc cacggctgga
    29101 aacaggggca acacataacc cggcagcttg cccgccgaga cggaaaagaa caggaacccg
    29161 aacgctaccc agatcagtag aaaccgccgg cgccggtcgc tgaacaaccc ccggcgcaac
    29221 aggaggacga acgcaggcgt ccacggcacg aagccggcca gcagcaccgg aacgtagaac
    29281 cagaacggac gtgcgtggag ctggtgcccg cttgaaaacc gggcgaagtg gtgacggaga
    29341 aagagttctt cgacgaacgg aaagccggtc ttccaggcga gcaccgcaaa ccatggaacc
    29401 gcaatcacca aaaacaccag aaccggcgcc ggatcgagaa accgccgcag acggccgcgc
    29461 catgcccaca gcaagggcag cgccagaacc aacggcacga gtcctttggc cagcactgcc
    29521 gccgccagca gaccggcggc gacagcaagc attcggcggg agccgcgctc cagagcgcgg
    29581 tagccaagca acatggcggc ggaaaatgtg acggccagcg gcacgtcggt gacggcgagc
    29641 tggctgtagg caagccagcc ggcggaactg ccgagaatga cagtcgcgaa cagtgctggg
    29701 cgcggcccga actcggtccg tagaaaccgg tagtagaaaa tcagaaagcc gagactcagc
    29761 agcaccaccg gcagcctggc cgccagttca ccccgcagcc cggcgcggtg cgctccgccg
    29821 atcatccagt aaagcagcgg aggtttctcg aaccagagtt ctccccacag gcgcggagtg
    29881 acccaatctc cggaaagcgc catgtcgcgg ccgatcgacg catagcgagg ctcgtcaggg
    29941 ccgatgaggc caatatccgt aagagcaaaa aagaagagaa aatagatagc aggaaccgcg
    30001 agccaccatt tccaagttcc ggaggacact caacaagtat aatgagcgcc atgtttcgct
    30061 tgggtgcaat aagtctcttt ttgcttccga tggccgcgcg cgccgatgtc gcgggttgcg
    30121 cctgcgaccc ggccgactcc gcttcgctgg aagcgcgcga gtgcgggctt acccgcgaag
    30181 ccctcaaaca gccagaagcg cctgccgtgt ttttcctaaa ggacatcaat ccgcgcaagc
    30241 cgaaccggtg gctggcgctt ccgcgccagg tcactaaggg cgtgtatcgc cttccggaaa
    30301 tgacagcgaa ggagcgcgcg gcactctgga ccgcggccat cgcccgcgcg aaggagttgt
    30361 ggggcgaagg ctgggggctg gcaatgaacg gcgacctcgt gcgcacgcag tgccagatgc
    30421 acgttcacct cgggaagttt gtggagggag ccgagagcga caacttcgtc gtcatccgca
    30481 gcgtcgaaga aatcccggcg ccggacggcg aaggcctgtg ggttcaccca acgagggacg
    30541 gcaagatgca cgtccatacc ggtgagatcc tgactgagac gatcctggtg aggtagcgcg
    30601 cccgtgtcac tactcgacat ctccaaggcg ccgacggccg agaacgccgt cattctgctt
    30661 caccccagtg acaacgtcgc ggtggcgcgc acggctttgc agggcggcca ggaggtaatc
    30721 gccggagggc gacggatcac cttaagggaa ccggttcccg ccggccacaa agtcgccgtc
    30781 tcgcgcatcg gcgccggcgc cctcgtccag cgctatggcc agaatatcgg accggcgagc
    30841 cgcacgatcg aaccgggcga gcacgtgcac acgcacaacc tcctgtacga ggagcggtcg
    30901 atcgactacg agttccccaa ggcggagatt ccccgccctg tggcaccggc cggcgggccc
    30961 gcgtttcacg gctacctccg tgaggacggc cgcgccggaa cgcgcaacta catcgcggtc
    31021 gtggctgcca gcaactgcgc cgcgcatacg gtggaattga tcgcccggag ctatgagagc
    31081 gaaaagctcc cgtccaatgt ggacggcgtt gtcgcgttcc ctcacggcga cggatgcggc
    31141 cacgcgatcg gccccgatac catactgctc caacgcacac tcagcggagt gctcgaccat
    31201 ccgaatgttt ccgcggccgt cgtgctgggg ctgggctgcg aggtgaacca gatcgaccac
    31261 tacctcggcg gagaggctgc gcggagcggc cgcctggccg gtatcacgct ccaggagagt
    31321 ggcggcacgc gggcggctgt cgaggctgcg cgccgcgaga tcgcaaaatc catcgagcgg
    31381 gcggagtccg agcgccgcgt cgagctgccc gcttcgaaga tcgtcctggg gctgaactgt
    31441 ggcggatcgg actcgttttc cggaatcact gccaaccccg cgctggggtt ctgctccgac
    31501 ctgcttgccg gtgcgggtgg aactccggtc ctggccgaga cgcccgagat cttcggcgcg
    31561 gaacacctgc tggttcggcg cgcgcgaaac cgcgcggtgg ccgagcgtgt gctcgcgttc
    31621 attcgaagct accaggagta cttgcgccgc ttcggcgaga gcttcgacga caacccgtcg
    31681 cccggcaaca aagaaggcgg cctctcaaac attctggaaa aatctctcgg agccgtcgcc
    31741 aagggcggta cgtccaatct gatggatgcg atcgattacg ccgagcgaat cgtgccgggc
    31801 ggccttgtat tcatgaacac gccgggctac gacccggtgt cgctggccgg tttggccgcg
    31861 ggcggtgcaa gcgtgatcgc cttcacctcg ggacgaggca gtgcgatcgg tttcccgagc
    31921 attccggtga tcaagatagc cagcaacagc cacacattcc gccggatgcg ggacaacatg
    31981 gacgtgaacg caggccggat caccgatggc gacgcggcga ttgaagacgt gggccaggag
    32041 atcttcgatt tgctcctgcg cgtggcttcc ggcgaacgaa cggcgagcga acgctggggg
    32101 cacaaggagt tcgtgccgtg gcgcataggg ccggtcatgt gaggagggag acagagaacg
    32161 tgaggggtga gataaaggcg ttgatgctgg cgctggccct ggcggccttg gcggccgcgc
    32221 aagggacgaa ggccgcggcg gcaaaaccgg ccggcaccgc gttcgacaag gtgaagttcg
    32281 aggcttacgt gcgccatctt tttgtttggg gtccgcatgt cgccgtgaac gtcggcgatc
    32341 cgaaaccgtc cgaattgccc gggttccggg aagtcgtcgt cactggagcg gccggtccga
    32401 ccagcttcaa ccaatcgttc ttcgtttccg ccgacgggcg gaaggtcgtc caggggcgcg
    32461 tcttcgacat ccaggagaac cctttccgcg aggaactggc gaagttgagg accggtatgg
    32521 gtccgagtct cggcacgccg ggcgcgccgg tggttctggt tctgtttacg gactaccagt
    32581 gcccgttttg ccgccaggaa gcacagatgt tacggcagaa cctgatcagc acgtacccaa
    32641 aggaagtccg gctctacctg aaggacttcc cgctcgaacc gatccatccg tgggccaaac
    32701 cggcggctat cgccggacgc tgcatctatg gccagaatga gcccgcgttc tggcaatacc
    32761 acgactgggt cttcgaccag cagcagacat tcacgccgga gaacctgcgc tcgaaggtga
    32821 tggactgggc gcagggcaag gagatcgata cccttcagct cggccggtgc atcgaagcaa
    32881 aggcgacgga agccgacgtc gacaactcga tcgccgaggg cagatctctc cagttgaacg
    32941 cgacgccgac gctgttcgtt aacgggcggc gcctgaccaa tgcgagctgg gagcaactga
    33001 aagccatcat cgattttgag ctggcgtatc aaaagacggc gaaaaacgcc ggcgacaacg
    33061 cctgttgcga ggtgaaactg ccatcgccgc tgcaatagca gtcttgaaaa cgcgcctcgg
    33121 ccgagcacga ctactgtttg ccgtggtgtt gtcgcttcgc gtcgcctgcg gggtcgagat
    33181 cgacccgggg cagtacctcg agcacatccg attcctggcg tccgaggaga tgaaggggcg
    33241 cggcactggt acgccggagt tggagcgggc cgccgattac ctggctgcac agttccgcgg
    33301 cttcggcgtt gaacccggca acggccaaag ctacttccaa tccttcccgg tgaccgcgca
    33361 cgcgcacctc ggcaaaaaca accagctcgt gtggcgcaac ggccggcggc aggcgctgaa
    33421 attcgactcg gatttccggc cgctcaactt ttcggagagc ggaacggtct ccggaaaggt
    33481 tgtgttcgcc ggttacggga tcaccgcccc cgagtacaat tacgacgact acgacgggat
    33541 cgacgtacgt aacaagatcg tgctcctgtt gcggcacgaa ccacaggagt tcgacgagaa
    33601 aagcgtcttc ggcggccggg tatacacgat ccacgcccag ttcgagagca aggccctgaa
    33661 cgccaagtcg cacggggcgc gcggcgtgat ctttatcaac gaccgtccca atcatccttc
    33721 ggaaagtgac gatctcgagc agttctccgg cgcgctcggc cccgcaagcc cggggatccc
    33781 gttcgtacag gtgaaggcgg aagtggccga agcttggatg acaagggctg gccggccgct
    33841 ggctgatgct gtaaaggcaa ttgacaagga cttgcggccg cagccgttcg cgctgccgga
    33901 ttcgttcgaa gtcgaaatga gagtagatat cgagcgggag acacgcatgg tgcggaacgt
    33961 catggcctac atgccgggcc agaccggcga gtacctcatc atcggggcgc actacgacca
    34021 cattgggctc ggcgagcagt attccatggc ctcctcggag gcgggcaaga agccacaccc
    34081 gggcgcggac gacaacgcat cggggacggc aggcgttctg gagctggccc gctggttttc
    34141 gaagcagcca cgaggcaggc gggggatcct cttcgtgacc tttgcggccg aggaaatcgg
    34201 cctgctcggg tcaagctact tcaccaacaa cccgccgagg ccgctcggga acgcggtggc
    34261 catgatcaac ctggacatga tcggacgccc gcgcgagggt aaggtatacg tcggcggcgt
    34321 ggggacgggc acaaccttcg gatcgatcgt caacggcatg aacggaaacg gcgcgtcgaa
    34381 gttgaacatc gatactacga cggccgacgg ttacggctcg agcgatcact tctcctttac
    34441 gccgaagcag attccggtgc tcttcttctt ttcgggaatg catggagact atcaccgtcc
    34501 cagcgacact tgggacaaga tcgacgcgcc cgccgccgcg gaactcctcg agtatgccgg
    34561 gcgtgtggca gaacagttgc ttgcagcccc ggaccggccc aggttcatcg gcgtcgcgca
    34621 cgcgtcgggc cactaatctt agcgccgcgt cctgacgatg gatgcggagg agatggcgca
    34681 atccacgttc gagtccgcac ggtgctggaa gttgccgccc ttgattctcc atccgttcgc
    34741 cgaggcttcg ggtccggaca ggttgacagc cagttcgagg gcaagcctca ttcttcaggg
    34801 attgctcccc ggagaagaat tcaatctgga ggagctgaat cgctggatcc tcgagggacg
    34861 cttctatgag gtccggatgc ttttctacat tggtaaggac ctcaaccgct ggatcgggca
    34921 gagcatggag gtagtcgagc gcgaggatgc gctccgggag gggggcgtcc gccgggagag
    34981 ttttgcggcg atgctggtgg aggatccgcc atccgccgtc caggagaagc tccggagttg
    35041 gggagtctcc gaccacaagg cgatctttcg aagggcgctc gggttgaacg cggtcttcgc
    35101 tgatgttccc ctccgcgaaa tgctcaccga cgaattcgtc cgccaccact tccgctacgc
    35161 cgaccgtatg ttcgagtgcc ggcaacaatc ggcgagattc acaccggttc acagcgaaaa
    35221 cttccctttc gaaatgtatg cctcgggcga gtacgctcga atgctcgagc gcgagtggga
    35281 ggtatagcac catctgcccg gccccacgtc cagggcgtaa aatagcggca tggagttctc
    35341 ccggttagtt cgcgtccgcc aacgttttcc cgaccgaagc atcaaggaca tcccagccga
    35401 ggtgcaacgg cagctcgcag gcgccgcgtt tggcgcccat ctgaagcctg gatcgaaggt
    35461 cgccatcggc gttggcagcc gcggcatcgc cagtatcggg acgatcgtgg gtgcggtggt
    35521 ggagtactgg aagtccctgg gaatgcaacc gtttatcttt ccggcgatgg gcacgcatgg
    35581 cgcggcgacc gcggagggcc aggcggcggt gctggccaag tacgggatcc acgacgccag
    35641 catgggctgc ccgattgtaa gctcgctcga cgtcgttgcc ctcggcgtga cgccggaagg
    35701 gatcgaaacc ttcatggacc ggcacgcttg ggagagcgat ggcgtgatgc tcgtgggccg
    35761 catcaagtgg cacacggatt tcatcggcaa gatcgagagc gggctgttca agatgatggc
    35821 catcgggctg gggaagttcg ccggcgcgga gcggtaccat acgcacgcct accggatggg
    35881 catcgaggcg atggtccgca gcgtcggccg gcaggtgctg aagtccggca agattctggg
    35941 cgggctggcg gtcctggagg acgccaacca caacgcagcg cagctcacgg cggtccccgc
    36001 ggcggacatg gagcgccagg aggaggagct gctggcgctg gcgaagtcct ggatgggacg
    36061 gattccggtg gaggctctgg acgtgctgat cgtcgacgaa atggggaagg acgtcagcgg
    36121 cgccggcatg gacacgaagg tcatcaaccg caacgccttc gcgaactaca atccctggcc
    36181 gaacgctccg atcatcgagc ggcttttcgt gcgcgacctg agcccgttga cttacggcaa
    36241 cgcggtcggc gtcgggatgg cggacgtggt gaccgaccgc ctggtcgaga agatcgactg
    36301 gcagccgacg cttatcaacg ccctgacggc ggcaacgctg gcggcggccc gcactccgat
    36361 ccaccttccc acggaccggc agtgcctgga aacgctggcc cgggcggtcg gcaagttcga
    36421 ttcgcgcgaa gtgacgatcg gttggatccg caacacgctg gagttgcgtg aactggcgtt
    36481 gagcgagaat ctgttgccgg agatcgaagc ggacccggcg ctcgaagtcc tcggtccccc
    36541 aagggagatc gagttcgacg cggagggcaa tctcctgccg gtgacagagg cagtacacgc
    36601 ctgaacgaag agctaccgct ccggccagag tgcgttgatc gtctcgacag tccgctccac
    36661 cagcacttcc ccgccttccg ggccgaccag tgagacatag gcctcggtcc catagcctcc
    36721 gcccgagagc gcccgggcag tggggacata catgcccctt ccggccaact ggacgataaa
    36781 cgtctgttcg gcggggctcc gcgcttcgat ttgcagtccg aagtccagat aaagctcgaa
    36841 cggatttgtg gcaatcgcca catcgcccag gcggaggaca tggagttcca tcgcatagaa
    36901 aggctcccga tcctggcgct cataccgttc caggacaccc ttgttccggt aaaggacgta
    36961 ggtccggtcg gcggcagcgt cccgggcggt gcgctccatc tcctggcggg cgttctcagc
    37021 ctcggctgcg ctgacctttc gcaccggaag gcgcagatcc tccaccttgt ggacaaacgg
    37081 cgggttgaag cggatgtccc tgttcgcgat ctcaaatgca tcagtgaccg cgctcacgat
    37141 ccggcgggcg gtctcttgcc gcgcagcgag accgcgccgc tcgaggttct tctgctcggc
    37201 ccgcttgtgg agcagcaggt gcggcgattg gtcgccagag gcgccggtca tcgggtagac
    37261 aaacaggtct ttcgagtacc gctttcgaag ctcgacccgc acatcgtgcc agaagtcggc
    37321 ggaaacgtag gacgcccctt ccactgactg ggagggacag gagacgttaa ttccgacgcc
    37381 ggtcagcttt gcatcatggt cccaaaagaa caacaggtcg actgagtgat cttcgtagcc
    37441 ctcgacgtgg ctgaaaccgg gaaggtccgt cttgccgtac atccgcgcgt ctccaccctt
    37501 gtaaacggcc cgccggttgt gcccgacgac cgcatggccg agcgcccagc ttacgcctgc
    37561 tggtttccgg ctcttccagg cctgttccgc acagtccgct agccgcccga caagaaactc
    37621 gatgtaatca gccggctgca tgacgccttc cggaatgtcg taaaggcctg gggtcatcgc
    37681 cggagccgtg tgagtatgag tggcgtgaag aaagaccttc cggacgtcga aaccggaaag
    37741 ccgcgatgcg agtcgctccc ggagtccctc caggatgccc gtccggatgg cgaccagatc
    37801 gcaggagatg aggatagcgc tctcaacggc gccagtctca ggtttccgct ccatggcgag
    37861 cgcggtggct gtcaccggat cttggacgtg cgtcgagacg cgcgtgcgca cctgtccttc
    37921 caaagccacc ggctggtcag gcgtaatgct tgccgaggcc cagccggcga ggaaccggtc
    37981 ttcggctgcc gcccaagtgg ccggaaagag gaaaattacg aggaggataa ctggacgatg
    38041 ccacatgtga ctgctctcgg ggcgcgatac actggcgcct gatcgatgat atcaagcggg
    38101 catcggagca aaggcaggca gtaagaacga tccggcgggg gaaaaggttt gggcctgccc
    38161 acccgccggt tttcgcattg aggacttcgg ggtaggtcta tccgccgccg gcaacgagca
    38221 cgctgccgat cttgctgaag atcgtgctga tgctggtcga gacgctgggc ataattgctc
    38281 ccgccgctac ggccacgaaa cctgccatca gagcgtactc gatgaggtcc tggccgcgcg
    38341 tgtcgttcca gaaattcctg aaccatttct tcatgctgtc tctcctctcc ctgcggtctg
    38401 ctatgggctg acagcctgaa agtaacaccc gggcggagac gttcaaatcc gcagaaacgc
    38461 ttagttaggg cgggaacaac gctgacggcg gcgagtcaga agtgcgcagt taggaaagcg
    38521 tacttcacgc cggatgccgc catgccaatc cagagtcatt gaggcaggat ctccatccgc
    38581 ttcgccacgc gcaccaggat ttccaccgcc cggttaagct gcactcgtgt gtgtgtcgca
    38641 gtgacgatcg tccgcacgcg cgccttgccc cgcggcacgg tgggaaagcc gatgccggtg
    38701 gcgaagacgc cttcctcgaa gatggctcgc gagtacgcgt gggctttcgc ggcgtcgccg
    38761 atcatgatag ctgtgatcgg agtctcgctg acgcctgtgt caaaaccggc agccttcagc
    38821 gcctccttga aataccgggt gttgctccag agattctcga tgcgctcagg ctcctgttcg
    38881 agaagctcga aagcggcaat gcaggaggcg gccacggcgg gcgggtgaga cgtcgagaac
    38941 aggaacgggc gggcgcgttg gtagagatag tcgatgaagt ccctgctgcc gcacacgtag
    39001 ccgccgagcg ctccgatcgc tttcgacagg gtgccgacct ggatgtggac gcgtccgtgc
    39061 aacccgaaat ggtcgaccgt gccgcggccg ttgcgtccga gaacccccga agagtgcgcg
    39121 tcgtcaatca tcatgattgc accatgcttt tcggcaagct ccaccagcgc cggcagcggg
    39181 gcgatgtcgc cgtccatgga aaagacgccg tcggtgatca gcagcttgcg ccccggttgg
    39241 ccggtgagcg cggcgagctt cgcatcggca gtggcgacat cgcggtgggg aaagacatgg
    39301 atcttcgcgc gcgacagccg gcagccgtcg ataatgctcg cgtggttcag ctcatccgag
    39361 atgatgtggt cctcggggcc gaggatggcc gaaacagccc cggcattcgc ggtaaagccg
    39421 gactggaaca cgacgcacgc ctcgacatgc ttgaactccg cgatgcggcg ctcgagttcc
    39481 atgtgaatcg ccatcgtgcc ggagatcgtg cggacagcgc ccgagccgac gcccagccgc
    39541 cgggtggcgt cgaccgcggc ctcgatcagg ttcgggtgat tcgtgagccc gaggtagttg
    39601 ttggaggcga ggttgatcac ctcgcggcca tcgaatcgcg agaccggcat gcacgccgtt
    39661 tcaagctggc ggagcgcctg gtatgttccc gccgcatgcc attcattcaa ctggtcggtg
    39721 aggaaagaga gaggattcga catggtatgg atgaactccc aaagggaatg ataacgtggg
    39781 gcctgtggta ccgcccgggc cgcccggtgc atttcgatct attaagacca ccatttgtgc
    39841 cccggattaa gcctgtgagg tcaacagcgt cgctattggc gaaacgtgcc accatcccgt
    39901 gaaacggctt gctcgcgaaa ctcaccatgg gttcgaggcc gtgttattag cgcctgttta
    39961 ttgtcgtcgg aatcgtcgtg gcaggcacgg tggcttgtca cctgcctgca ggagggtcag
    40021 ccatgtcgaa cgtgtatggg ccgcgcttgg tgacgccgcc tgtgtcgggt catcccttct
    40081 gcgcgagcac cgaggccgcc agtgagaaga agacttggct ggggctgcgc cgcaaaatct
    40141 tcacaggagt aattgcacgc gacggccaag ggcgtcttcg cacggaattc ggggttcgag
    40201 ggcagccgct ggtaatcggg attgtcgatc cagtcagcct gaaagcgtat gctttggatc
    40261 cgagacggga aactttcttc gagctgcccg agtgtcctcc gcagccagtg ctccctgcac
    40321 tgcaaaagcc gccaggcgct gagacacaac gtatcgaggg cttggaatgc ttcaggagag
    40381 tcatccccga gttgatcgaa gaagcctgga tttcgtctga actccagcac gttgtgttgg
    40441 aaagagcagt tgacggtggc gtgaggttca cgtggcggat gtacgatatc cgcctcggcg
    40501 aacctgaccc tgctctgttc agaatcccac ccacgtacaa acaagcttga acggccgcct
    40561 acgccattga gacccaaaag ccacagctag ctccgtcgtg aaacaattgt cctgaatgag
    40621 gagggtcgac ttcgtcttct tcgatgccgg gggcgggcac cgcgcggcgg ccactgcgct
    40681 aaagcatatc gttgagcagg agcagaggcc gtgggacgtg caactgatga accttcagga
    40741 ggtcctccat cccctcgacc tgttccggaa gctcaccgga ctgcgcctcc aggacatcta
    40801 caacctgatg ctcaagaaag gctggacgct cggctcagcg caactcgtac ggccaatgca
    40861 gtggctgatc cggctctatc gcccaaaaca ggttccgctc cttgaagcac actggcgcga
    40921 acgccagccg gatatggtgg tctccttcgt gccgcacttc aaccgcgcat tgaaggaggg
    40981 catgctgcgt gcgcggccgg aaggtcgttt cgtgacagtt ctcaccgatc tcgccgatta
    41041 ccctccgcat ttctggatcg aacgccagga acaaaatctg atctgcggca gcgatcgcgc
    41101 cgtggagcag gcgcgggcgt tgggccatac cgacgagcgc atcttccgcg tgtcaggcat
    41161 gatcctgaac cctcgtttct acgatccggt catcgtggac cgccgggccg agcgggcgcg
    41221 gctcggcctc gacccagaaa aacccaccgg cctcgtgctg ttcggcggtc aaggctcgtg
    41281 ggtgatgcgg gagatcgccc ggaagctcga ccgctcgggt ttggacctgc aactcatcct
    41341 gatttgcggc aagaatgaga agctcgcgcg ggcgctacgc gaatacaagc cccgcattcc
    41401 gacgttcgtg gaagggttca ccagcgaggt gccgtactac atgcacctct cggacttctt
    41461 catcggcaaa ccgggcccgg gcagcatcag cgaggcgctg gcgatgaagc ttccggtgat
    41521 cgtcgaacgc aacgcatgga ccctgcccca ggagcgctat aacaccgagt gggtgctgga
    41581 gcaccgcgtg ggccgcgtcg taccgaactt ccggaacatc gacatcgtgg ccgcggaaat
    41641 gctagccccc ggcgagctcg cccgttaccg tgcaaacgcc tcagccatcg acaaccgcgc
    41701 ggtgttcgag atccctgcta tattgcagag gattctcgaa tactcatgaa ataccgactt
    41761 ctttctgtca ccttgatggg cggggcgatg ctggccgctc aaagcccggc ccccacgccg
    41821 gaatcccggc tgctcggctg gatggaccgt atcgcgcagg agcagctcga ccggcgcgcc
    41881 gcggaaatcg cgaaggtccg tacaccggag caggccgacg cacgcaagca atacgtacgc
    41941 gcgaagattc tcgagctgct cggtgggctg cccgactata gtggtccgct caacgccaac
    42001 gtcaccggcc gcatcgacaa gggcaagtac gtcatcgaga acgtcgtctt cgaaagcctg
    42061 ccgaaagtct gggtgactgc gaacctgtac cgtccggctc aggcgggccg ctatcccgcc
    42121 gtgctcctgc cgctcggcca ctgggatcag gggaaacccg ccgtgcaatt gatcgcggcc
    42181 aacctcgcgc tcaaaggctt cgtcgtcctt gtctacgatc cgatcggcca gggcgaacgg
    42241 ctgcaagcct acgatcagcg gaccggcaag tcgctcgcgg gcggatccac cgatcagcat
    42301 ttcctgcccg gcgcgcagag cattctcgcc ggagagagct ttgcgcgata tcgcatctgg
    42361 gacgccaagc gcgcgctcga ctacctcgtc agccggtccg aggtcgattc cgagcgcatc
    42421 ggctgcaccg gctgctccgg cggcggcacg ctgacgacct acgtctccgc gctcgacccg
    42481 cgaatcaagg ttgccgcgcc ggcctgctac atgaattcgt tccggattct gttccccgga
    42541 ccggtcggcg attccgagca gagcctccca aacttccttt cgtccggact ggaccagacg
    42601 gattatgtgg agctcttcgc gccgaagccc tggctgatcg cgaacacagt agacgatttc
    42661 ttcccgctcg agggcgctcg gcaggtctac caggaggcga aacgctggta ctcggtctac
    42721 ggggccgagg agagaatcgc gtgggtgatc ggaccgggag gccacggcac gccgctaccg
    42781 gttcgcgagg cgatctacgg ctggatgatc cgctggctga aggacggccg gggagacgcg
    42841 cgcgaggaag aggtcgagtt cctttccgat cacgagcttc tcgcgaccaa aaccggccag
    42901 gtttcgaccg gcctcgggtc gcgcgacatt tacgaagcca ttcgcgatgg agcgaagagc
    42961 agaatgcagt ccttcagcgc cggcgaactg ctcaacacgc tcagaaactg gatgcggccc
    43021 gtcgccgaga cgccgctcaa tgtaaaggtt gtcgccgaga cgttgacggc cgacatcacg
    43081 acgcagaccg tgatcttcga gaccgagcgc gggctcgaaa tcggcggagc actgatcatt
    43141 ccacggggcg ccgtacgaag gccgggggtc gtggtcgtcg agactggacc gccgccgtcg
    43201 ccgctcgcgc ttcagcttgc ccggaaaggg gccgtggtgc tggcgctgtc gccgcgcggg
    43261 ctgccgtgga acgtcagcat ccggcagttg cccggcgact ggctggcggc cacgcgcgcg
    43321 tggctgatcg gcaagaacat gccggggctg cgcgcgtacg atatcaagcg tggtgtggat
    43381 ctgctggcgg cgcgggccga cgtcgatccg gcttcgatcc gggcggtggc gcgcgatgcc
    43441 gccggcgtgt ggctgttgat ggcggcggcc accgacgcgc ggatcggagg cgtctggctg
    43501 gaccgcaccc cgcacagcct acgagcggta at
//
LOCUS       DATIFQ010000272         4139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_89408, whole genome shotgun sequence.
ACCESSION   DATIFQ010000272 DATIFQ010000000
VERSION     DATIFQ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89408"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..577
                     /locus_tag="VLE22_12660"
     CDS             77..577
                     /locus_tag="VLE22_12660"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB15303.1"
                     /translation="MTKPVLAPEFCQVAWVVKDIAAAERFFVDTIGISRFLHIDNLAA
                     KDTEGTYLGKPGNWVCNLYIACAGDTQIELIQPVSGASMFQESLDRHGDAVQHVAYWL
                     DDAGYAAAAAHLESSGYPEIQSFRLPIARVGYFDTRRAIGVVTEIVGANKEGHEFRRN
                     LKAGNF"
     gene            complement(720..1883)
                     /locus_tag="VLE22_12665"
     CDS             complement(720..1883)
                     /locus_tag="VLE22_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HSB15304.1"
                     /translation="MPWSETTKMRERMRFVLDAEGELFTMVELCERYGISRMTGHKWL
                     RRFRQDGVGALEDRSRAPLHCPHRTDPEVVEALVEARRQHPYWGARKLILWLAKRQPS
                     LRLPAASTAGDILKRVGLVKDRRRRRNLPHPGPPTTEGIYPNQLWSADFKGQFRTRDA
                     VYCYPLTVADSFSRYLLGCRSQLTTATIPTKKNFERVFRTYGLPEAIRTDNGNPFSCA
                     TAISRLSRLSVWWIRLGIRPELIQPSHPEQNGRHERMHKTLKAETTRPPAANARAQQR
                     LFDSFREEFNTERPHERLENETPASIYRPSPRPYPERLPPLEYPGHFEVRRVSRNGGI
                     RWKKGWVNISTSILEQNVGLEEVDDGVWSLYFGSVLLGRFHEDDLTLHGARLD"
     gene            2063..2533
                     /locus_tag="VLE22_12670"
     CDS             2063..2533
                     /locus_tag="VLE22_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15305.1"
                     /translation="MRPTPARILLSAIAIGSLAAGVLTSQLIFFVVALGASILLGTSL
                     STNRHSLTHAINRFRNHTVEVRLWGAPPPGLSGATLVLTSVSAIGAGAHVFFNVQGGG
                     GLHLKVAQPQDASLAPECVVIGSPKYLQWNGKKLRNSGSAPAVAIALSEQTVRE"
     gene            2785..3075
                     /locus_tag="VLE22_12675"
     CDS             2785..3075
                     /locus_tag="VLE22_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15306.1"
                     /translation="MAYGIIHFFPGGTKDQYDASLAAVHPGKNTLPKGQVFHAAGPST
                     GGWTIVAIHESKESWESFRDKTLMPKLQAGVPGGFKTSPHEQAFEVYYQQKS"
     gene            3196..3573
                     /locus_tag="VLE22_12680"
     CDS             3196..3573
                     /locus_tag="VLE22_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15307.1"
                     /translation="MVRLVIGRQGESGLGFDLPEVLKALGPRVLASAWAIDGLHYVST
                     DERNVSGFQAEEGVRIPGAKLLSGISNLLQVLDGEFRAFEASREPWVIVRAIDSSWWE
                     VESDDSVALSAIRERFRNVDEQS"
     gene            3667..4023
                     /locus_tag="VLE22_12685"
     CDS             3667..4023
                     /locus_tag="VLE22_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15308.1"
                     /translation="MALTSGARSAAERVEAALLGLGVAAMLFSGWHVFRFWNSLAYDS
                     TDHKIEPFGEGSALAVICLLIICTIVGCVALLRSVSNHRIVSRLARWSYAVLVLYGSW
                     RLYRWAAWYFMVSHNL"
BASE COUNT          736 a         1241 c         1335 g          827 t
ORIGIN      
        1 cagcggacgc gcttgcgcgc gccgctgagc cgcaagccgt taggccgcat cggcacatag
       61 aaacaggagt ccagacgtga caaagccagt acttgctccg gagttctgcc aggttgcctg
      121 ggtcgtcaaa gacatcgcgg cagccgaaag gtttttcgtc gacacgatag ggatcagccg
      181 cttcttgcac attgacaacc ttgccgcaaa ggacaccgag ggcacctacc tggggaagcc
      241 cgggaactgg gtctgcaacc tgtacatcgc ctgcgcgggc gatacgcaga tagaactgat
      301 ccagcctgta tcgggggcca gcatgttcca ggagtcacta gatcgccacg gtgacgccgt
      361 tcagcacgtg gcgtactggc tggatgatgc tggctacgcc gccgctgccg ctcatctgga
      421 gtcctcgggc taccctgaga tccagagctt ccggttgccg atagcacgcg tgggctactt
      481 cgatacgcgt cgggcaattg gagtcgtcac ggaaattgtc ggagccaaca aggagggcca
      541 cgaattcaga cgcaatctga aggccgggaa cttctgagaa ggttgtgagc agccggggag
      601 atagcttaca gaatagaccg ggaagatggt ttacacaacg ggatggaaag tagggctctg
      661 gggtcctggt tcgttctcct ttctctagcg cgtcgtagca ggcggtactc cgttctctct
      721 caatcgagtc gagctccgtg gagtgtcagg tcgtcctcgt ggaatcggcc gagcaggacg
      781 gagccgaagt agagggacca gacgccgtcg tcgacctctt ccagaccgac gttttgctcg
      841 aggatgctgg tgctgatatt gacccagcct ttcttccagc ggatgcctcc gttacggctg
      901 acgcggcgga cttcgaagtg gccgggatat tccaacggcg ggagccgctc cggatatggt
      961 cggggagacg ggcggtagat ggaggcgggg gtttcgttct cgagcctctc gtgtggccgt
     1021 tcagtgttga actcttcgcg gaaggagtcg aagaggcgtt gctgggctct ggcattggcg
     1081 gcgggcgggc gggtcgtctc ggctttgaga gtcttgtgca ttcgttcgtg gcggccgttc
     1141 tgctcggggt gtgagggctg gatcagctcg ggccggatgc ccaggcggat ccaccagacg
     1201 gagagacggg acaggcggct gatcgcggtg gcgcaggaga aggggttgcc gttatcggtc
     1261 cggatcgcct cgggaagccc gtaggtgcgg aagactcgct cgaagttctt cttggtcggg
     1321 atcgtcgcgg tagtcagctg agaccggcag cccagcaggt accggctgaa gctgtcggcc
     1381 acggtcaggg ggtagcagta gacagcgtcg cgggtcctga actggccctt gaagtcggcc
     1441 gaccagagct ggttggggta gatgccctcg gtggtgggcg ggccgggatg cgggagatta
     1501 cgacggcggc gccggtcttt gaccagaccc acgcgtttga ggatgtcgcc ggcggtgctg
     1561 gcggcgggaa gcctcaggct cggctgccgc ttcgccagcc acaggatcag cttacgagcg
     1621 ccccagtagg ggtgctggcg tcgagcctcg accagcgctt ccacgacctc tggatcggtc
     1681 cgatgcgggc aatgcaacgg cgctcgactt cgatcctcca gggctcctac cccatcctga
     1741 cggaatcgcc gcagccactt gtgacccgtc atccggctga tcccgtaacg ctcgcacagc
     1801 tccaccatcg tgaacagctc cccctccgca tccagcacga accgcatccg ctctcgcatc
     1861 ttcgtcgttt cgctccaggg catccctcac cccctccaac ccatcctgtg cgaaactgtc
     1921 aaccatgtcc ccggtctatt ctgtaaacca tgtacccggt ctgtacctgc agcggcctaa
     1981 cccgcggctt cagcggacgc cttcggcgcc gctgagccgc aagccgttcg gcgagcgccg
     2041 ccatggccat cgtcggtagc tcatgcgtcc tacacccgcg cgaatccttt tgtcagccat
     2101 cgcgataggc tcgctcgcgg cgggtgtcct cacctcacag cttattttct tcgtggttgc
     2161 gctaggagcg agcatcctgc tcggaacgag ccttagcaca aatcggcact cgctgaccca
     2221 tgcgatcaac cgcttccgga atcacacggt ggaggtccgg ctctgggggg ctccgcctcc
     2281 tggcctttct ggcgccacgc tcgtcctgac gtcggtaagc gcgattggcg ctggtgctca
     2341 cgtctttttc aacgttcagg gcggcggtgg gctacatctt aaggttgctc aaccacaaga
     2401 cgccagcctc gcgcccgagt gcgtcgtcat cggttcacca aagtacctgc agtggaacgg
     2461 caagaagctt cgtaatagtg gcagcgcacc ggcagtcgcc attgcactat ccgagcagac
     2521 cgtaagagaa tgacgggaga gctcgagagc ctgggtgcat cgatgaatgt cctgccgaag
     2581 acggcgctgc agcggccgcg cttgcgcgca ccgctgagct ttgggacgtt cggcggacgg
     2641 tggtgaaacg aggattttgg cccccagggt ggtgctggtc ggctctgcga ggcaccggtg
     2701 tcgtggtgtc ctcctggccg tttcgggtgt atcctgcccg cttaaactgt gtacagggac
     2761 ctcgcgtccc agaggagaat cgaaatggca tacgggatca tccacttctt tcccggcggt
     2821 accaaggatc agtacgacgc gtcgcttgcc gcggtgcatc ccggcaaaaa cacactcccc
     2881 aagggtcagg tcttccacgc cgccgggccg tcgactggcg gctggaccat cgtcgcgatc
     2941 cacgagtcta aagagagttg ggagtccttc cgcgacaaga ccttgatgcc gaaactgcaa
     3001 gcgggcgtgc ccggcggctt caagacgtcc ccgcatgagc aggccttcga agtctattac
     3061 cagcagaaat cctgagcggc ccctgggcaa tagggggtcc tctgacgccg ccgaacgacg
     3121 cgcttcagcg gactcgcgcc ggcccttcgg tccggcgctc gccgctgagc ttgtaaacgt
     3181 taggcggctg aagtcatggt ccgcttggtc atcggccggc agggagaatc ggggctcggc
     3241 ttcgaccttc ccgaggtcct aaaggccctg ggaccgcggg tattggcctc tgcctgggca
     3301 attgacggct tgcactacgt ctctacggac gagagaaacg tctcgggctt tcaggcagag
     3361 gagggcgtga ggattccggg ggctaaattg ctctcgggta tctcgaatct ccttcaggtg
     3421 cttgacggcg aattccgagc gttcgaggcc agccgagaac cctgggttat cgtgagggcg
     3481 attgatagct cgtggtggga agtcgaatcg gacgacagcg tagcgttatc agccatccgt
     3541 gagcggttcc ggaatgtcga cgaacagtcc taagccgcct aacccgcggc tgcagcggac
     3601 gccgtcggct tcgccgccgt cgccgctgag ccgccatccg ttcggcgata ccgtctcgag
     3661 aacgtcatgg cactgacttc aggcgcccgc agcgcggctg agcgggtcga ggcggcgcta
     3721 ctcgggctcg gcgtcgcagc catgcttttc agtgggtggc acgtcttccg attctggaac
     3781 tccttggctt acgacagcac cgatcataag atagagccct ttggcgaggg ctcagctctc
     3841 gccgtaatct gtctcctcat catctgcacc atcgtgggat gtgtcgcttt gctacgatcc
     3901 gtgagtaatc atcggatcgt ctcccgccta gcccgttggt cgtacgcagt gctggttctg
     3961 tatggatcct ggcggctcta ccgctgggcg gcttggtact tcatggtttc gcacaacctg
     4021 tgacggcaag cggcgccggt gaacagcatg cccgctaaaa agccgttagg ctgggagttt
     4081 gggcgctgag gccctgatcc gaatcaagca ggaggcgttc gaatgcgctt cagcaaaaa
//
LOCUS       DATIFQ010000273        19173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1621021, whole genome shotgun sequence.
ACCESSION   DATIFQ010000273 DATIFQ010000000
VERSION     DATIFQ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19173
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621021"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VLE22_12690"
     CDS             <1..411
                     /locus_tag="VLE22_12690"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB15309.1"
                     /translation="PHAKSGTMGWLASHIANIPSWAAVAINQDSLDMAPAGAEPPRTP
                     QAGSRKELLEIFDKNVADARAAIAGADDNRLLAPWTLLSNGQTILTMPRIGVLRSFVM
                     NHSIHHRAQLTVYLRLNDLPVPALYGPSADEGGM"
     gene            complement(428..2677)
                     /locus_tag="VLE22_12695"
     CDS             complement(428..2677)
                     /locus_tag="VLE22_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15310.1"
                     /translation="MSTRRHQLLLACSAFLVSFVSVKPSLAGAFPWKAPTGITLPQAV
                     TPAAGGESAWRNEGGVWLLNAARAGLLESPAWWTGNRPPRGEVIVLEVDYLDDFAHPV
                     RAEVYSALGTTRPYSELHRFGGAADGRWKTARIPASSDFTFRSASNALQFRLTPAGGP
                     LKARAFRLTAPLPDEEERYNAETRAWVAREQRRAETSPRYFNLAQKAVLPGDWSRKPL
                     VPFRRNWMDLVRPISAPQAGEAGAPLRVRLFRNDDEPVQLGVYANARDLRNVRVSVAP
                     VKDAAGNTVVEVIPRVAEYSKVRGQMIPSYLIELFPQRLWPAYPFDVPAGHSHMVWLV
                     VLTKEATARPGTYTTMVRLQADGVEEFAVPLTVEVLNARLLTMEEAGLKLGGCTTGLV
                     PEFELEALRNYNHNMVNIWYASIRPLLSKNSGSFDMDFRIMDDWMAAAKRAGISDLVY
                     FLGGNPYGFPQTFGFPRDLANTLLGLDDAGWKRLAMENPHVVPEKIAPLMVEWTRRFG
                     DHARSNGWPNVVLTPFDEPAKYVQYQSSLGMLTFIKPQFKEQIRLLRKGDPKAQIYGS
                     IHHYDPGIEFLEDVDIFCTNAVSENWNLPDEVRAARKILWEYSGNHDRGLPATARYTF
                     GYYFAAHNSRGSLVWAYNWGNRFDTLDGNNWMYVWNTPFDLIPTPYMEGLREAWDDRR
                     LLETLKRTAAGKGVNLDTFLGRLFGEISTARGEGGTDTVDDFWDRAKNDAMMDQWKDR
                     MVDKLLSLQ"
     gene            complement(2690..5152)
                     /locus_tag="VLE22_12700"
     CDS             complement(2690..5152)
                     /locus_tag="VLE22_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15311.1"
                     /translation="MLRRGLHSLRGFIAVLLLLAGALPSASSNRPPRCASGSLITALD
                     LKIVPPEGGQARRLRDVNAIPAGWRILYAPGYLSSNDPEDAKISVVMAPAEPGRTIEI
                     SDPFRANEPGEWKAPFRIGVLGVVFGPQGLDRKKVRNMVAEDADLISQLAEYAEKSTQ
                     TENLIQALAAYEKQPNSGQNLEAALTGFAGAWSLPMSRLDAGAPLDQQAALLIRTLNP
                     AFSSLDPLSQQSGRRFQQSAGLAASVAGLFWGPTVGVAAGGTALFLNLRNMMFPGTDF
                     RSTFAQASSENVALCAARETQKPRTRLAYLWAVKVPNIEAPKLELARDAHVATGLRSE
                     VALRPAEFEGRFLLRARDWRVTPVGGGEDIPVHVLPGSAAGKLSSIQLDLAAAEVPPR
                     EYRLAGRWDWTDFQVDGAIHVYRVLPLTQARPTPETTDRLIAGAGPGQLEIKGADFQF
                     VRKATLRRQGEPSVKATGLELLNPEDIDPGPRPVLHVAVDTSSLSPGAYTLALDQQGG
                     LVTDIPVRVLPPHPVLDGLPLRVNLGEPQQLLTLRGRGLDRIEAASAEKAELQLLAGN
                     ATKRQLRVRLLDPARKGDSIGLRLKVEGVHAELPVDHALFVAGPRPRITAVTVSAPPV
                     ASVELREGELPAGVPRTFAVRATGLDGTTRLQAGCNDPSRRLAPVSLRRGEQRNGAEM
                     QQTGPETLFLSLEPGMIGPPGCRLRLLVETDGEGVSDPAPLGTVVRLPRIEKFEFTDE
                     QTAAGLYIAVLEGYDLDAIAQTGWNSQEGHPVQALPSPVPGQTPRQSLRVALPWPAPS
                     PRAPLYVFLHEETSGRLTKARL"
     gene            complement(5161..6147)
                     /locus_tag="VLE22_12705"
     CDS             complement(5161..6147)
                     /locus_tag="VLE22_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HSB15312.1"
                     /translation="MTRLDAIVAGAGPTGLACGIELQRRGLNAVLIEKGCVVNSLYNY
                     PTNMVFFTTPELLEIGDIPMTSLNEKPTRTEALKYYRRVADHYRLQIRQYERVEHIAG
                     EDGGFTVHTEDRNGCPRQYVTSKVILATGFYDRPNLLGIPGEDLEKVIHYYREPHPYY
                     GHDVAVIGGKNSAAIAALELHWSGARVTLIHRREGLSDSVKYWIKPNVENRIANGEIT
                     AHFNSHVAEIRPEEILVRTPGGDLWLKNDFVFAMTGYHPDLEFMSTHGIPVDRETGRP
                     YTDPETLESRRKGIYLAGVIVAGMHTNEIFIENGRFHGRKIAEAIASDLRKR"
     gene            complement(6144..8786)
                     /gene="alaS"
                     /locus_tag="VLE22_12710"
     CDS             complement(6144..8786)
                     /gene="alaS"
                     /locus_tag="VLE22_12710"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HSB15313.1"
                     /translation="MTGHEIRRKFLDFFAQRGHRVVPSSSLVPANDPTLLFTNAGMNQ
                     FKDVFLGREKRDYTRAASSQKCVRAGGKHNDLDNVGYTRRHHTFFEMLGNFSFGDYFK
                     ADAIAFAWELVTKDYGMPQDRLYITVFREDDEAELLWQKVTGFPKSRIFRLDEKDNFW
                     QMGDTGPCGPCSEIHFDLGPEASEPGHEDEKFPSDCGGRYVEIWNLVFMQYDRDINGV
                     LTPLPRPSIDTGMGLERVAAILQGKLSNYDTDLLRPIIERAADMFGVVYGEDARTDTA
                     LRICADHSRAAAFLIHDGVVPSNEGRGYVLRKIMRRGMRHARLIGVHEPFLYTLTGFV
                     AELMQPAYPELMESVSRVARVVKDEEHRYQTTFQVAERVFHDEAKAAAAGVLAGPVAF
                     KLYDTYGLALDEQDEMARDYGLTIDRDGFEREMERQRERARASWKGAQQAQIAPVYEA
                     LAEKRRTRFLGYDTLESASSRVVGLVVNQSLADEVAPGASAELVLDETPFYAEAGGQV
                     GDTGALYSKETGAQVARVESVYPALAGLSVHRIVAVAPVRAGDLLRAQVDESPRRSTM
                     RNHTATHLVHAALRTVLGTHVKQAGSVVEPSRLRFDFTHYAAMDPAEIEEVERLVNEQ
                     ILRNTDVQTDLMDLDSAITTGAMALFGEKYGDTVRVVSVPGFSKELCGGTHVRRTGDI
                     GLCKIVSEGSISAGARRVEAITGEGALHRFQHASDSLARISQIVRASETELVDHVEKL
                     IAQQRAIEKQLDQMKNRVAQAQLSELEGQARTLKGVRVLAGRVDGLDRQQMRVLADSL
                     RNKWKSGVVVLASIDDSNVAIVSAVTKDLTGKVHAGKLAGSVAQMVGGKGGGRPDMAE
                     AGGKDVAALPAALDAVYTQVEGML"
     gene            complement(8792..9484)
                     /gene="pyrF"
                     /locus_tag="VLE22_12715"
     CDS             complement(8792..9484)
                     /gene="pyrF"
                     /locus_tag="VLE22_12715"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSB15314.1"
                     /translation="MTRQPIIIALDFESAEAARELVSKLGDAVSFYKVGMELYASAGM
                     EFVRELIASRKRVFLDMKFYDIGETVKRAVAQVAMSGVAFCTVHGSKAVMRAAVDGRG
                     DSSLKLLAVTVLTSFDQNDLADLGYPCSVSELVDLRVAKAMEAGVDGLVCSPLEVAAV
                     RGIAGPKAILVTPGVRSAGEGKGDQKRVATPAKAIRNGANYLVIGRQVTRAKDPRAEV
                     LRICEEIATVSQ"
     gene            complement(9537..10391)
                     /locus_tag="VLE22_12720"
     CDS             complement(9537..10391)
                     /locus_tag="VLE22_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB15315.1"
                     /translation="MKLKSLILLLVLCSFAVAQDAKLRGGNMLACETYSYRDLIRDGK
                     LDMTTVPEFYKQQGIKGISYNDMFFKTLDDAYLDQVKAAVKKADRVVTCYVIEGNLAM
                     ADETKRREQIEADKQKMRAAAKLSAPVVRINVGRTDKQENSDDTVGVDRVVAAFKELL
                     PLAKELNLKISIENHGGVSKTAANIVKIIKATDPKWVGSLVDFGNFPPENRYAEIEAV
                     APFAFVTHVKIMEFDANGEASDYDFPRVLKMLKKNKYKGPISIEYEGKLDAVEGVQKT
                     KALILKYW"
     gene            complement(10462..10659)
                     /locus_tag="VLE22_12725"
     CDS             complement(10462..10659)
                     /locus_tag="VLE22_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15316.1"
                     /translation="MNFVLEIFQRDFGQLDPSQKVFLVTVILGCAVLVGYSICRLLRY
                     SRKLESVDSQLSKLSNVSRVA"
     gene            complement(10670..11629)
                     /locus_tag="VLE22_12730"
     CDS             complement(10670..11629)
                     /locus_tag="VLE22_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15317.1"
                     /translation="MIPLLNGNGKQTREKNLRANYLERLREIAEDTRYGGDALLRDAA
                     VGELSEVSVLEQTHSEVALPEPWQVESEESKGSNEAFDSDEFRLLAEQVAPRCIDALT
                     EAFRGVHRLAAGDLGKAEAVTNQVIALSDEIRHINGEISSLRQSTEFLTGAERDLVTR
                     AAVLEGQLAECATSDRQVIADLAELTNAQARDRESQTLANSATQEQLNRLEQTLTERL
                     SALENRFTASLETVSLVSENLTAIAQALQTHFETSARINVSLSGVQEAQEVLRRRLDN
                     QAEVMRKLCASEQERGNQLQTTLQKMKEMTVEFFMPAPLPEDL"
     gene            complement(11857..12126)
                     /locus_tag="VLE22_12735"
     CDS             complement(11857..12126)
                     /locus_tag="VLE22_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15318.1"
                     /translation="MDVNWPASIAATAIFWLAGIVLSFWPKQVQKYYLGFLDRHQNTA
                     RFNPFVDWMRTPSYVMYLRVIGVVCFTVSAFLMVCLAVAIRSSLP"
     gene            complement(12130..13410)
                     /locus_tag="VLE22_12740"
     CDS             complement(12130..13410)
                     /locus_tag="VLE22_12740"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HSB15319.1"
                     /translation="MRFALVLFTAAALLPAAAAPFEAGVARVEITPSTLLPMYGYGNR
                     KGPAVGYHDPLHAKALVLAAGSDRVAIVTLDIGMFYSERLRRRVTDELGIPVLILASS
                     HTHSGPSFIKNREDAPDPSSEQQAYLKELEEKLFGIVKEASANMFPARIGLARGALRA
                     GYNRLVLREDGRARANFDNREHVAYGPVDPEFVLLKIEDISGAARALLVHYACHAVVL
                     QGSNRKYSADYPGVLQARVEEAMKGVQCMFVQGGAGDINPLSMGQTGEDEEDFKAVRR
                     MGDALAREVLTTANTIRTTEPARPEIRAKAELLTFADRWNKNETVEAGITTVLINREI
                     AIAAVPGEPLHRLQTFWKANADVAWPLFYGYSVSSAETWPGYIPDLRSAAYGGYGADV
                     STNIEVGAAERIMQRHLINLYGLRDMWLDKPGKN"
     gene            13446..16538
                     /locus_tag="VLE22_12745"
     CDS             13446..16538
                     /locus_tag="VLE22_12745"
                     /inference="COORDINATES: protein motif:HMM:NF044450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15320.1"
                     /translation="MRLTILLATLSLLATLPLADAQQAGVMEATWGLDVLGRILGDSR
                     NVVVPVDKFQLEGFEVDNVSEYRSPSVTRKGEDARITVTAAKAGTFYPGIIFYDSRGN
                     ERIELRVAGVVRGTVIADADDNRERLYFTAEPVAFRGGETIELRALTADGIYRTENIV
                     LLAERPAPKRREFTITQVEPRSEATARSATAAITWITSWPAACTVEWGENKRHVEELA
                     VNNHRVRLADLEPEQSYRFRIVARTPEGGEVTSTWREFRTEPTLAPAGSVTQARVLLT
                     AASPSGAVAAGGLLPVTAGVPFPEGALGSDENLRVLDQAGRELPLQSRMQARWKDGSV
                     KWVLLDFQGRPDTAYTLEYGSDVRRRQGLSRLSVKDEPEQVEVVTGPLRIRVSKQKYG
                     VPGSVWFDTSGNGRFEPNEAILAPGTLELRDASGALFSSFAPPEEVVVEESGPVRAVV
                     RVSGGHYAEDGRRLFRFITRLHAYAGLPFVRVEHTFENDSSAAEFTSIRSLVLRLPLT
                     PAGAGKIEGQLGGTGAALTAPVHLAQHRDDWYDISQGRRLADGRRSAGLARWSDGSRS
                     VQLAMRDFWQNYPKDLTVSANGFELGICPPLAANEYDDAKGTMDEHRLYYYLQGGVYK
                     FRQGVSKTHDLWLSFGRAAEAQAPPTSPLRTVAPAAWYEAGKAFGEISVPKQNGILAR
                     YDEAFARSFDGYLGNRETNREYGMLNFGDWWGERVINWGNSEYDTQHAFFLQFVRTGD
                     WRPFLAGEEMEWHNRDVDTIHHHSDKSRVGGVYLHCVGHTGDYYSTSPFEGKGITRGG
                     TIISHTFIEGHLDYYFLTGDRRSLDTAMRIADRVNTYETRNYDFTNCRNPGWHLVLNM
                     AAYNATNDPFYLNAARIIVERVLERQTRNGGWERQMVPGHCTCTPHHRGNAGFMVGVL
                     LTGLKHYYDATADELVATSIVRGSHFLIDDMWEPEVNGFRYTSCPRSNKGAWSNFLLF
                     DGIAFAHRRTHDAKLGEILQRGTIPALDTMNSMGKGFTQYTRVAPHFIGYLADLQEAE
                     "
     gene            complement(16554..17315)
                     /locus_tag="VLE22_12750"
     CDS             complement(16554..17315)
                     /locus_tag="VLE22_12750"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15321.1"
                     /translation="MLETVDLSQSIDRETYDVQLTRRQIQLRELGYQVYRQKRPVVMV
                     FEGWDAAGKGGAIKRLTERLDPRGYSVFPISAPEGEDKTHHYLYRFWRRLPESGQISI
                     FDRSWYGRVLVERVEGFCTEEAWRRAYREINSFERQLVEFGAVLLKLWIHISREEQLR
                     RFEERERIAYKSWKLTQEDWRNRDKWPAYQEAVEDMLLKTSTATAPWTVVEGNDKLWA
                     RVKVCATAVDVLSRELKYEPREPLRKGGNNRKKSK"
     gene            complement(17315..18076)
                     /locus_tag="VLE22_12755"
     CDS             complement(17315..18076)
                     /locus_tag="VLE22_12755"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15322.1"
                     /translation="MSKEEYRSVLPALQQRLYDLEKACWDHRIASILLFEGWDASGKG
                     SAIATLTERLDPRGFKLYPIQPPRTYEQNHPWLWRFWSRTPNYGEMAIFDQSWYRRVL
                     QDRVGKLISKREASSAFQDIVEFEQMLADDGTVIVKFWLHISRKEQKKRFEALEKDPL
                     ESWRVTKEDWARHRKYDNYLKAAEEMLARTESEYGPWTIVEATSRWFARRKIFETVIR
                     ALEARLGDAAPPVRPVGGKSRAKVGNRQKSRKGRP"
     gene            18316..>19173
                     /locus_tag="VLE22_12760"
     CDS             18316..>19173
                     /locus_tag="VLE22_12760"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HSB15323.1"
                     /translation="MTRCQFLSAPVTALLVLPLFASSAGAAEFFVYFGTYTRGPSKGI
                     YVSRLDTATGKLTEPNLAAETPNPSFVALHPNRKFLYAVSEGGKAGAVSAFAIDPKTG
                     RLAFLNKVSSQGGGPCHLNVDKTGKNVVVVNYGTGSTAVLPVEPDGKLREASSFIQHK
                     GSSADKSRQEGPHAHSVNFSADNRFAIVADLGLDQLLVYKFDAAKGTIAPNDPPYATV
                     APGSGPRHFAFHPGGKFAYAINEMKCTVTAFSWDAKRGVLKEIQTIGTLPAGFTGEND
                     TAEVQVHPNG"
BASE COUNT         3487 a         5963 c         6081 g         3642 t
ORIGIN      
        1 ccgcacgcga agtcgggcac gatgggatgg cttgcatccc acatcgcgaa tatcccatcc
       61 tgggcggccg tggcaatcaa tcaagattcg ctggacatgg cgccggcagg agcggaaccg
      121 ccgcgcactc cacaggcagg cagccggaaa gagctcctgg aaatattcga caagaacgtg
      181 gccgatgcga gggcggccat tgccggcgcc gacgacaacc ggctgctcgc gccgtggaca
      241 ctgttgtcca acggtcagac gatcctgacg atgccgcgca tcggggtcct ccgtagcttc
      301 gtgatgaacc acagcattca ccaccgcgcg cagcttacgg tttaccttcg gctgaatgac
      361 ctgccggtac cagccctcta tgggccgtcg gcggacgaag gcggaatgta aaggcaggct
      421 cccgcggtca ctgtagcgaa agcagcttgt cgaccatccg gtccttccac tggtccatca
      481 tcgcgtcgtt cttggcgcgg tcccagaagt cgtccactgt gtcggttccg ccctcgccgc
      541 gggcggtgga gatttcaccg aatagccgcc ccaagaacgt gtcgaggttc acccctttcc
      601 cggccgccgt gcgcttcagg gtttcgagaa gacgccggtc gtcccaggcc tcacgcaggc
      661 cctccatgta aggtgtagga atgaggtcga acggagtgtt ccagacgtac atccagttgt
      721 ttccgtcgag cgtatcgaac cgattgcccc agttgtaagc ccataccaat gagccgcggc
      781 tgttatgggc ggcgaagtag tagccgaacg tatagcgggc ggtcgcgggc aggccgcggt
      841 cgtggttgcc ggagtactcc caaagaatct tgcgcgcggc gcggacctcg tccgggaggt
      901 tccagttctc gctgacggca ttcgtgcaga agatgtcaac atcctccagg aactcgatgc
      961 cgggatcata gtggtgaatc gagccataga tctgggcctt cggatcgcct tttcgcaaca
     1021 ggcggatctg ctccttgaat tgcggcttga tgaacgtgag catgccgaga ctggattgat
     1081 actgcacgta cttcgcgggt tcgtcgaacg gcgtgagcac cacattcggc catccgttgg
     1141 aacgggcgtg gtcgccgaaa cggcgggtcc actcgaccat gagcggggcg atcttctccg
     1201 gcactacgtg ggggttctcc atagcgagtc gtttccagcc agcgtcgtcc aacccgagga
     1261 gagtgtttgc taggtcgcgc ggaaaaccga acgtctgcgg aaagccgtag gggttgcccc
     1321 cgaggaagta gaccagatcg ctgatgcctg cccgcttcgc cgccgccatc cagtcgtcca
     1381 tgatgcgaaa gtccatgtcg aaggagccgc tgttcttcga caagagcggc cggatgctcg
     1441 cgtaccagat gttgaccatg ttgtggttgt agttccgcag cgcctcgagc tcgaattccg
     1501 ggacgaggcc agtggtacag ccgccgagtt tcagtccggc ctcctccatg gtcagcaacc
     1561 gtgcgttcaa tacctcaacg gtcagaggaa cagcgaactc ctcgacaccg tccgcctgga
     1621 gacgcaccat ggtggtgtac gttccaggac gtgccgtcgc ttccttggta agaaccacaa
     1681 gccaaaccat gtgactgtgc ccggcaggca cgtcgaacgg atacgcgggc cacagccgct
     1741 gtgggaacaa ttcgatgaga tagctcggaa tcatctggcc tctcaccttg gaatattccg
     1801 cgacccgggg gatgacttcg acgaccgtgt tacccgcggc gtctttcacg ggcgcgacgc
     1861 tgacacggac attccgcaaa tcccgcgcgt tggcgtagac gccgagttgc accggctcgt
     1921 cgtcatttcg aaacagacgc acgcgcaacg gagcacccgc ttcgcctgcc tggggcgcgc
     1981 tgatcggacg cacaaggtcc atccagttcc ggcggaaggg aacaagcggc ttgcgggacc
     2041 agtcgcccgg aaggaccgct ttctgagcca ggttgaagta cctcggagag gtctccgcgc
     2101 gccgctgctc acgagcgacc caggcgcgcg tttctgcgtt atatcgttcc tcttcgtcgg
     2161 ggagcggagc ggtgaggcga aacgctctgg ccttaagcgg gccgccggcg ggcgtgaggc
     2221 ggaattggag ggcgttcgac gccgagcgga aagtgaaatc ggaggacgcc ggaatgcggg
     2281 cggtcttcca gcggccgtcg gcggcgccgc caaagcggtg caactcgctg tagggccgcg
     2341 tcgtgccgag ggcgctgtaa acctcggcgc gaaccgggtg ggcgaaatcg tccaggtaat
     2401 cgacttcgag gacgatcacc tcgccgcggg gcggccggtt tccggtccac catgcgggcg
     2461 actcgaggag tccggcccgt gcggcgttga gaagccaaac gccgccctcg ttgcgccaag
     2521 cggattcacc gccggcggcg ggcgtgacgg cctgtggaag cgtgatgccg gtgggcgcct
     2581 tccagggaaa ggcaccggcc aacgagggct taacgctgac aaaggagaca aggaaagcgg
     2641 aacaggctag gagtagttga tgtcggcgtg tgctcatgat cgcaactatt cagagccgcg
     2701 ctttggtcag gcggccagag gtttcttcgt gcaaaaagac gtacagggga gcacggggcg
     2761 agggagcggg ccagggcagg gcaactcgca gcgattgacg cggcgtctgc ccgggaaccg
     2821 gggagggaag cgcctgcacc gggtggccct cctggctgtt ccagccggtt tgcgcgatgg
     2881 cgtcgagatc gtatccttcg agtaccgcaa tgtagaggcc ggcggcggtc tgctcgtctg
     2941 tgaactcgaa cttttcgatg cgcggcagtc ggacgaccgt accgagcggc gccgggtcgg
     3001 agaccccttc accgtcggtc tccaccaaga ggcggaggcg gcagcccgga ggcccgatca
     3061 tgccgggttc gagagaaaga aagagggttt ccggcccggt ctgctgcatc tcggcgccgt
     3121 tgcgctgctc gcctcggcgg agagataccg gcgccagcct gcgcgatggg tcattgcagc
     3181 cggcctggag ccgggtagtg ccgtcgaggc cggtggcgcg gacagcaaac gtcctgggca
     3241 ccccggccgg cagttcaccc tcgcgaagtt cgacggaggc caccggggga gctgaaacgg
     3301 taacggcagt aatccgtggg cgcggacccg cgacgaaaag cgcgtggtcg acgggaagct
     3361 ccgcatggac gccctccact ttcaggcgaa gcccaatgct gtcgcctttg cgagcgggat
     3421 cgaggagccg cacgcggagc tgccgcttcg tcgcgttgcc ggcgagcagt tgcagctccg
     3481 ccttttcggc cgatgccgct tcgatccgat ccagtccgcg gccgcgcagt gtcagcaact
     3541 gctggggttc gccgagattc actcgcaaag gcagcccatc gagaacgggg tgcggcggca
     3601 gcactcgcac cgggatgtcg gttaccagcc cgccttgctg gtcgagagcc agcgtatagg
     3661 cgccgggcga taaggaactg gtgtcaacgg cgacgtgcaa gacgggccgt gggccgggat
     3721 cgatgtcctc aggattcaga agttcgaggc cggttgcctt gactgagggt tcgccctgac
     3781 ggcgcagggt ggctttgcgg acgaactgga agtccgcgcc cttgatttcg agttgcccgg
     3841 ggccggcgcc cgcgataagg cggtctgtgg tctcgggggt aggacgagct tgggtcagag
     3901 gcaacacacg gtagacgtga atcgctccgt ccacctgaaa gtccgtccaa tcccagcggc
     3961 cggccagacg gtactcacgg gggggcactt cggcggcggc gaggtccagt tgaattgacg
     4021 acaattttcc cgctgcgctg ccaggaagca cgtgcacggg gatgtcttct cctccgccca
     4081 ccggcgtaac ccgccagtca cgggcgcgga gcaggaagcg gccctcgaac tcggccggac
     4141 gcaaagcgac ctcacttcgg aggccagtgg cgacatgtgc gtcgcgggcc agttccagtt
     4201 tcggcgcttc gatatttgga accttcaccg cccagagata ggcgagccgc gtgcgaggct
     4261 tctgcgtttc gcgggcggcg cagagcgcga cgttctccga gctggcctgc gcaaacgtag
     4321 agcggaaatc ggtaccaggg aacatcatgt tccgcaggtt aagaaacagc gccgtgcctc
     4381 ccgccgccac tcccacggtc ggaccccaga acaaacccgc caccgacgcc gccaagcccg
     4441 ccgactgctg gaaccggcgg ccgctctgct gcgagagagg atctaacgag gagaaggcgg
     4501 ggttgagagt gcggatcagc aaggccgcct gttgatcgag gggggcgccg gcatcgagcc
     4561 gcgacatcgg cagactccac gcaccggcga agcccgtgag cgccgcttcc aggttctgtc
     4621 ctgaattggg ctgcttttcg taggcggcca gcgcttgaat gagattctcg gtctgcgtgg
     4681 atttctcggc gtattcggct agctggctga tcaggtccgc gtcctcggcg accatgttgc
     4741 gcaccttctt gcggtccaga ccctgtgggc cgaagacgac acccagcacg ccaatccgga
     4801 agggggcttt ccactcgccg ggctcattgg cccggaaggg atcgctgatc tcgatggtcc
     4861 gtcccggttc ggccggggcc attaccaccg aaatcttggc atcttcgggg tcattactgg
     4921 agaggtatcc gggggcgtag agaattcgcc atcccgcggg gatggcgttc acgtcgcgca
     4981 ggcggcgggc ctggcctccc tccgggggca cgatcttcag atccagggcg gtaatcaacg
     5041 agccgctggc gcagcgcggc ggacggttgg aagaagcgga cggcagcgcg cccgccagca
     5101 aaagcagaac agcaataaat ccgcgcaggc tgtgaagccc gcgccgtaac aagccagaca
     5161 tcatctcttt cgcaggtccg aagcgatggc ctcggcgatc ttccggccat gaaaacggcc
     5221 gttttcaatg aaaatttcgt tggtatgcat gccggcgacg atgacgccgg caagataaat
     5281 tcccttgcgg cggctctcca gggtctcggg atcggtgtag gggcggccgg tctccctgtc
     5341 gacggggatg ccgtgcgtgc tcataaattc gaggtcgggg tggtagccgg tcatggcaaa
     5401 gacaaagtcg ttcttcagcc acagatcgcc gcccggcgtt cggacgagaa tctcctccgg
     5461 ccggatttcg gccacgtgcg agttgaaatg cgcggttatt tcaccgttcg cgatccggtt
     5521 ctccacgttg ggcttgatcc agtatttcac gctgtcggag aggccctcgc ggcgatggat
     5581 cagcgtgacg cgcgcgccgc tccagtgaag ttccaacgcg gcgatggcgg cggagttctt
     5641 gccgccgatc acggccacat cgtggccgta atagggatgc ggctcgcgat agtagtggat
     5701 gaccttctcg aggtcttcac cggggatgcc gagcaggttc ggccggtcat agaaccccgt
     5761 cgcgaggatc actttagacg tgacgtattg ccgcggacat ccgtttcggt cctcggtgtg
     5821 gactgtgaag ccgccgtcct ccccggcgat gtgctcgacg cgctcatact gccggatctg
     5881 aagcctataa tggtccgcca cgcggcggta gtacttaagc gcttccgtac gtgtgggctt
     5941 ttcgttcagc gacgtcatcg gaatgtcgcc gatttcgagc agctccggcg tggtgaagaa
     6001 gaccatattg gtcggatagt tgtacagcga gttcaccacg cagccttttt cgatcaggac
     6061 ggcattgagg ccgcggcgtt gcaactcgat gccgcaggcg agaccggtcg ggccggcgcc
     6121 cgcgacgatc gcgtcaagcc gtgtcacaac attccctcga cttgcgtgta cacggcgtcg
     6181 agcgccgccg gcagcgccgc cacgtccttt cctcccgcct cggccatgtc aggccgccca
     6241 ccgcctttgc cgccgaccat ctgggccacg gatccggcga gcttgcctgc gtgaaccttg
     6301 ccggtcaggt ctttggtgac cgccgagacg atggcgacgt tcgaatcgtc gatagaagcg
     6361 agcacgacga cgccggactt ccacttgttg cgcagcgaat cggcgagcac acgcatctgc
     6421 tgacggtcca gaccgtccac gcggccggcg agcacgcgga cgcccttcaa cgtgcgggcc
     6481 tgtccttcaa gctcggaaag ctgcgcttgc gccactcggt tcttcatctg gtcgagttgc
     6541 ttctcgatgg cccgctgctg ggcaatgagt ttctcaacgt gatccaccag ctcggtctcg
     6601 gaggctcgaa cgatctgcga gatccgggca agcgagtccg aggcgtgctg gaaacgatgg
     6661 agcgcgccct cgccggtgat ggcctcgacc ctgcgagctc ccgccgagat gcttccctcg
     6721 gagacgatct tgcaaaggcc gatgtcaccc gtgcgccgca cgtgggtgcc gccgcacagc
     6781 tccttactga agccggggac ggagacgaca cggacggtgt cgccgtactt ctcgccgaac
     6841 agcgccatgg cgccggtggt gatggcgctg tccaggtcca tcagatcggt ctggacgtcg
     6901 gtgttccgga gaatctgctc gttgacgagg cgttcgactt cctcgatctc cgccgggtcc
     6961 atcgccgcgt agtgcgtgaa gtcgaagcgg aggcgcgacg gctccacaac gctgcccgct
     7021 tgcttgacgt gggtcccgag gaccgtccgc aatgccgcgt gaaccagatg cgtcgcggta
     7081 tggttacgca ttgtagagcg ccgcggggat tcatccacct gggcgcgcag cagatcaccg
     7141 gcgcggaccg gcgcaacggc cacgatgcga tggaccgaga gcccggcgag ggcggggtag
     7201 accgattcga cgcgagccac ctgggcgccc gtttccttgg aatagagtgc gccggtgtcg
     7261 ccgacctggc cgccggcttc ggcatagaac ggcgtttcgt ccaaaacgag ctcggcactg
     7321 gctccggggg caacctcgtc cgcgagggac tggttcacca ccaggcccac cactcgggag
     7381 gatgccgact ccagcgtgtc gtagccgagg aagcgtgtac ggcgcttttc ggcgagcgct
     7441 tcatagacgg gcgcaatctg ggcttgttgt gctcccttcc agcttgcgcg ggcacgctcg
     7501 cgctggcgct ccatttcacg ttcgaagccg tcgcggtcga tcgtaagacc gtaatcgcgc
     7561 gccatctcgt cctgctcgtc gagggcgagg ccgtaggtgt catacagctt gaacgcgacc
     7621 ggccccgcaa ggacgccggc agcggcggct ttggcttcgt cgtggaaaac ccgctcggcc
     7681 acctggaatg tggtctggta gcggtgctcc tcatccttca ccacacgggc gacgcgcgaa
     7741 acgctctcca tcagctcggg atacgcgggc tgcatcagct cggcgacgaa cccggtgagg
     7801 gtgtacagga acggctcatg aaccccgatc aggcgcgcgt gccgcatgcc gcgtcgcatg
     7861 atcttgcgaa ggacgtagcc ccggccttcg ttcgacggca cgacgccgtc gtgaatcagg
     7921 aacgcggcgg cgcggctgtg gtcggcgcaa atgcgcagcg cggtatcggt acgggcgtcc
     7981 tcgccgtaca cgacgccaaa catgtccgcc gcgcgttcga taatcggccg cagaagatcc
     8041 gtgtcgtagt tggaaagctt gccttgcagg atcgccgcga cgcgctcgag tcccatgccg
     8101 gtgtcgatgg acgggcgggg caacggggtc aggacaccgt tgatgtcgcg atcgtactgc
     8161 atgaacacaa ggttccagat ttcgacgtaa cggccgccgc agtcgctcgg gaacttttcg
     8221 tcctcgtggc ccggctccga cgcctccggg ccgaggtcaa agtgaatctc cgagcacggc
     8281 ccgcacggtc cggtgtcgcc catctgccag aagttgtcct tctcgtcgag acggaaaatc
     8341 cggcttttcg ggaaaccggt caccttctgc cacagcagct ccgcttcgtc gtcctcgcgg
     8401 aacacggtaa tgtacaaacg gtcctgcggc atcccgtagt ccttcgtgac cagctcccag
     8461 gcaaaagcaa tcgcgtcggc cttgaagtag tccccgaagg agaagttgcc gagcatctcg
     8521 aagaacgtgt ggtgccgacg cgtgtagccg acattgtcca ggtcgttgtg ttttccgccg
     8581 gcccgcacgc acttttggga ggaggcggcg cgcgtgtagt cccgcttctc gcggcccagg
     8641 aagacgtcct tgaactggtt catgcccgcg ttggtgaaca gcaatgtcgg atcgttggcc
     8701 ggcaccaatg acgagctggg aaccacacgg tggccgcgct gagcaaagaa atcgaggaat
     8761 ttccggcgga tttcgtgacc tgtcacagat tttattgtga cacggtggcg atttcctcgc
     8821 agattcgaag cacttccgcg cgcggatctt tcgcgcgtgt gacttgccgt ccaatcacca
     8881 gatagttggc gccgttcctg atcgccttgg cgggcgtcgc aacgcgcttc tgatcgccct
     8941 tcccctcgcc cgctgagcgc acgcccggcg tcaccaggat ggccttcggt ccagcgatgc
     9001 cgcgcacggc ggccacttcg agcggcgagc agaccagtcc gtccacgccc gcctccatcg
     9061 ccttggcgac gcgcaggtcc accaactcgg agacgctgca cggatagccg agatctgcga
     9121 gatcgttctg gtcgaaactc gtgagcacgg tgacggccag cagcttcagg gaggaatctc
     9181 cgcggccgtc gacagccgcc cgcatcaccg ccttgctgcc gtggacggtg cagaaagcga
     9241 ctccgctcat cgccacctgt gcgacagcgc gtttgaccgt ctcgccgatg tcatagaact
     9301 tcatgtcgag gaatacgcgc tttctcgagg cgatcagctc ccgcacgaac tccatgccgg
     9361 cggaggcata cagttccatg cccactttgt agaaagacac ggcgtccccg agcttggaga
     9421 ccagctcccg cgctgcttcg gcggattcaa aatccagggc gatgatgatg ggctggcggg
     9481 tcatagttcg gttatcgggc gacccgccgg cgggtcgccc gccccgacca tgcagtctac
     9541 cagtatttga ggatgagggc cttggttttt tggacgccct cgaccgcgtc gagcttgcct
     9601 tcgtattcga tcgagatcgg tcccttgtac ttgttctttt tcagcatctt cagaacgcgc
     9661 gggaaatcgt aatctgatgc ttcaccattt gcatcgaact ccatgatttt aacatgggtt
     9721 acgaatgcga acggagcgac tgcctcgatc tccgcgtagc ggttctccgg cgggaaattg
     9781 ccgaagtcga cgagcgagcc aacccacttc ggatcggtcg ctttgatgat tttcacgatg
     9841 tttgcggcgg tcttggaaac gccaccgtgg ttttcgatcg aaatcttgag gttcagctct
     9901 ttcgccagcg ggaggagctc tttgaaagcg gcgacgacgc ggtcgacgcc gacggtgtcg
     9961 tcggagttct cctgcttgtc ggtgcggccc acgttgatgc ggacaacggg ggcgctcaac
    10021 ttcgcggcgg cgcgcatctt ctgcttgtct gcctcgatct gctcgcggcg tttggtctcg
    10081 tcggccatcg cgaggttgcc ttcgatgacg tagcaggtca ccacgcgatc cgccttcttg
    10141 acggcggcct tcacctgatc gagataggcg tcgtcgaggg tcttgaagaa catgtcgttg
    10201 tacgagatgc ccttgatgcc ctgctgcttg tagaactcgg ggacggtggt catgtccagc
    10261 ttgccgtcgc ggatcagatc ccggtagctg tacgtttcac aggcgagcat gttgccgccc
    10321 cgcagcttcg catcctgggc gacggcgaag gagcagagca cgagaaggag aatgagggat
    10381 ttcagtttca taagaagacg atgatagcgt gaaaatcaaa cgcgccgtac cctgagacgc
    10441 atcgggggaa tatgagtgtc cttacgctac gcgggagacg ttgctcagct tcgagagttg
    10501 tgagtcgacc gactcaagct tacgcgagta acgcaggagg cggcatatgg agtacccgac
    10561 cagcacggcg cagccgagaa tcactgtcac caggaaaact ttctggcttg gatccagttg
    10621 gccaaaatct ctttggaaga tttcaagcac aaagttcacg ggctcttcct cacaggtcct
    10681 ccggcaaggg ggctggcatg aagaattcca ccgtcatttc cttcattttt tgcagcgttg
    10741 tctgcaactg gttgcccctc tcctgttcgg aagcgcagag tttccgcatc acctcagcct
    10801 gattgtccag ccgtcgccgg aggacttctt gcgcttcctg gacgccggaa aggctcacat
    10861 ttatccttgc actggtttcg aagtgcgtct gcaaagcctg cgcgatggcg gtcagattct
    10921 cgctgacgag tgagactgtc tccagcgacg ccgtgaatcg gttctccagg gcgctgagtc
    10981 gttcggtcag ggtctgttcc agtcggttca actgctcttg cgttgccgag ttggccaggg
    11041 tctggctctc gcggtcccgg gcctgggcgt ttgtgagttc cgcgaggtct gcaatgacct
    11101 gccggtcgct tgttgcacac tcagcaagct ggccttccag gacggccgcc cgggtcacca
    11161 agtcccgctc cgctcctgtg aggaattctg tgctttgacg cagcgaggag atttcgccgt
    11221 tgatgtgccg aatttcgtcc gacagggcga tcacctggtt tgtgactgcc tcggccttgc
    11281 ccaggtcgcc ggcggcaagc cggtgcaccc ccctaaacgc ttccgtcaaa gcgtcgatgc
    11341 accttggagc cacctgctcc gccagcagcc gaaactcgtc ggagtcgaac gcctcgttgg
    11401 atccctttga ttcctcagat tccacttgcc aaggttcagg cagtgccacc tcagagtgcg
    11461 tctgctcgag aacggacacc tcactcaact cccccacagc agcgtctcga agcagtgcat
    11521 cacctccgta gcgagtgtct tcggcaatct cgcgcagccg ctccaagtaa ttcgcgcgaa
    11581 ggttcttttc tctcgtctgc ttcccattgc cgtttagaag aggaatcatc gttttaacct
    11641 atgcgtgcca atttaagatc attcgcgatt cccctcaatg gagctccagt acgtttttga
    11701 ataggaagaa aattcacttt gttctagtac cccaggttag aagagaatgc gtctatctta
    11761 ttcacttctt ggcacttccg tacggcgtcc tataggaaag aagttccaga aaaggcgaac
    11821 aaacagcgaa tcctggcgtt ccgatcccac gcaaaactac ggcaagctgc tgcggatagc
    11881 cacagcaagg cagaccatca ggaaggcgga aacagtaaag cacactacgc cgatgacccg
    11941 taagtacatc acgtacgacg gcgtgcgcat ccagtcgaca aaagggttga atcttgcagt
    12001 gttttggtgg cggtccaaga acccgagata gtatttctgg acctgctttg gccagaacga
    12061 aagtacaatc cccgctaacc agaagatggc cgtggctgcg atggatgcgg gccagttaac
    12121 gtccatcgct cagttcttcc ccggtttgtc gagccacatg tcccgcaggc cgtacaggtt
    12181 gatgagatgg cgctgcatga tgcgctccgc ggctccgact tcgatgttcg tgcttacgtc
    12241 ggcgccgtag ccgccgtacg ccgccgaacg gaggtccggg atgtaccccg gccatgtttc
    12301 ggccgagctg acggagtacc cgtaaaacag tggccaggca acgtcggcgt tcgccttcca
    12361 gaaggtttgc agacggtgca gaggctcgcc cggcacggcc gcaatggcga tctcacggtt
    12421 gatcaggacg gtcgtgatgc cggcctcgac cgtctcattc ttattccacc ggtccgcaaa
    12481 cgtgagcagc tcagccttcg cgcggatctc gggccgagcg ggttcggtcg tgcggatcgt
    12541 attcgcggtg gtgagcactt cccgggcaag cgcgtcgccc atccggcgga cggccttgaa
    12601 gtcctcttcg tcctccccgg tctggcccat gctgagcgga ttgatgtcgc ccgctccgcc
    12661 ctggacgaac atgcattgca cgcccttcat cgcctcttcc actctggcct ggaggacgcc
    12721 gggatagtcg gccgagtact tgcggttcga tccctgcaat acgaccgcgt gacaggcata
    12781 gtggaccagc agcgcccggg ccgcgccgga gatgtcctca atcttgagga ggacgaattc
    12841 cgggtcgacg ggaccgtagg cgacgtgctc gcggttatcg aagttggcac gcgcacgacc
    12901 gtcctctcgc agcacaaggc ggttatagcc cgcgcgtagt gcaccccggg ccagtccgat
    12961 ccgcgcaggg aacatgtttg cggacgcttc tttgacgatt ccgaagagct tctcctcgag
    13021 ctccttgagg tacgcctgct gctccgacga tggatcgggc gcgtcctcac ggttcttgat
    13081 gaatgacggt ccggagtgcg tgtgcgagct tgcgaggatc aacacgggaa tgccaagttc
    13141 atccgtaacc cgccggcgca ggcgttccga atagaacatg ccgatgtcaa gcgtgacgat
    13201 agcaacgcga tcagatccgg cggcaagcac cagcgctttg gcatggagcg gatcgtggta
    13261 tccgacggcg ggccccttcc ggttgccgta tccgtacatc ggcagcagcg tcgaaggcgt
    13321 aatctcgacg cgcgccacgc cggcctcaaa aggggcggcg gcagccggca aaagcgcggc
    13381 ggcggtgaag agaacgagcg cgaatctcat gcttggtata gtatctgaca cgaagcggtc
    13441 cggctatgag gctcacaatt cttcttgcga ctttatctct gcttgcgacc ctcccgttgg
    13501 ctgacgccca gcaggccggt gtgatggagg cgacctgggg gctcgacgtg ctcggccgga
    13561 ttttgggtga tagccggaat gtcgtggtgc cggtcgacaa gttccagttg gaaggcttcg
    13621 aggtcgacaa cgtgtcagag taccgaagcc cgtctgtcac ccgaaaaggt gaggatgcac
    13681 gcatcacggt cactgcggcg aaagcaggaa cgttttaccc cgggatcatt ttctatgact
    13741 cgcgcgggaa tgagcgcatc gagctccgcg tggccggcgt cgttcgtgga acagtcatcg
    13801 ccgatgccga cgacaaccgc gagcggctct acttcactgc cgagcctgta gctttccgcg
    13861 gcggcgaaac gatcgagctg cgcgcgctga ccgcggacgg catctaccgc acagagaaca
    13921 tcgtgctcct ggccgagcga cccgctccga agcgccggga gttcacgatc acgcaggtgg
    13981 agccgcgctc cgaggccacc gcccggtcgg ccacggctgc gatcacctgg atcacaagct
    14041 ggccggcggc gtgtacggtc gagtggggcg agaacaagcg gcacgtggaa gaactggccg
    14101 tcaataacca tcgtgtgcgg ctggcggacc tcgaaccgga gcagagctac cggttccgca
    14161 tcgtggcgcg gaccccagag ggcggcgaag tgactagcac ttggcgggaa ttccggaccg
    14221 agcccacgct ggcgccggcg ggttcagtga cgcaggcgcg cgtgctgctc acggcggcaa
    14281 gcccgtcggg cgcggtggcg gcgggcggac tgctgccggt gaccgctgga gtcccattcc
    14341 cggaaggcgc actcggttcc gacgaaaatc ttcgcgtgct cgaccaggcg ggcagggaat
    14401 tgccgttaca gtcccgcatg caggcacgct ggaaggacgg tagcgtgaag tgggtgctgc
    14461 tcgacttcca gggcaggccc gacaccgcct acacgctcga gtacggctcc gatgtccggc
    14521 ggcggcaggg gctgtcccgc ctgagcgtga aggacgagcc cgaacaggtc gaagttgtca
    14581 ccggaccgtt gcggattcgt gtcagcaagc agaaatacgg cgtacccggc agcgtgtggt
    14641 tcgacacgag cggcaacggc cgcttcgagc cgaacgaagc gatccttgct ccgggcaccc
    14701 tcgaactgcg ggacgcaagt ggagctctct tttcgagctt tgcgccgccg gaggaagttg
    14761 tcgtggagga gagcgggcca gtacgggccg tggttcgcgt cagcggcggt cattacgcgg
    14821 aagacggccg caggctgttt cgcttcatca cgcggctgca cgcctacgcg ggactgccgt
    14881 tcgtgcgcgt ggagcacaca ttcgagaacg actccagcgc cgccgaattc acgagcattc
    14941 gaagccttgt attgcgactg ccgctcacgc cggccggggc ggggaagatc gaagggcagc
    15001 tcggaggtac aggcgccgcg ctgaccgcgc cggtccacct cgcgcagcac cgcgacgact
    15061 ggtacgacat ttctcagggg cgccgcctcg cggacggcag gcgctcagcc ggcctcgcac
    15121 gttggagtga cggcagccgt tcggtccaac tcgcgatgcg cgatttctgg cagaactacc
    15181 cgaaagacct cactgtctcc gcgaatgggt tcgagctcgg catttgccct ccgctcgccg
    15241 cgaacgagta tgacgacgcg aaggggacga tggacgagca ccggctctat tactacctcc
    15301 agggcggcgt gtacaagttc cggcaggggg tcagcaagac gcatgacctg tggctctcgt
    15361 tcggccgggc ggcggaagca caagcgcctc cgacgtcccc cttgcgaacg gttgcgccgg
    15421 cggcgtggta cgaggcgggc aaggcgttcg gcgaaatctc cgtgccaaag cagaatggaa
    15481 tcctcgcacg ctacgacgag gccttcgctc gctcctttga cggctatctc ggcaaccgcg
    15541 agacgaaccg cgaatacggc atgctcaatt tcggcgactg gtggggcgag cgcgtgatca
    15601 actggggcaa cagcgagtac gacacgcagc acgccttctt cctccagttt gtgcgcaccg
    15661 gggactggcg tccttttctc gccggcgaag agatggagtg gcacaaccgg gacgtcgaca
    15721 cgatccacca ccacagcgac aagtccaggg taggcggggt gtatctgcac tgcgtgggcc
    15781 acacgggcga ctactattca accagcccgt tcgaggggaa gggaatcact cgcggcggga
    15841 cgataatctc gcacacgttc atcgagggcc acctcgacta ctacttcctc acgggcgacc
    15901 ggcggtcgct cgacacggcg atgcggatcg ccgaccgcgt caatacctac gaaacgcgca
    15961 actacgactt caccaactgc cgtaatcccg gctggcacct ggtgctgaac atggccgcct
    16021 acaacgcgac gaacgatccg ttctatctga acgcggcgcg gatcatcgtg gagcgcgtgc
    16081 tggagcggca gacgcggaac ggcggctggg agcggcagat ggtgccaggg cactgcacgt
    16141 gcactcctca tcaccggggg aacgcggggt tcatggtcgg cgtactgttg acggggctga
    16201 agcattacta cgatgcgacc gccgacgagc ttgttgcgac atcgatcgta cgcggctcgc
    16261 atttcctgat cgacgacatg tgggaaccgg aggtcaacgg attccgttac acgtcgtgcc
    16321 cgcgctcgaa caaaggagcg tggtcgaact tcctgctgtt cgacggcatc gcgttcgcgc
    16381 accgacggac gcacgacgcc aaactcgggg agattctcca gcgcggcacc atccccgcgc
    16441 tcgacacgat gaacagcatg gggaaaggtt ttacgcagta tacccgcgtg gcgccacatt
    16501 tcattggcta tctcgcggac ttgcaggagg cggagtaaga gccgggcgcc ggtctacttg
    16561 gacttcttcc ggttgttgcc gcccttccgg agcggttccc gcggttcgta tttcagttcg
    16621 cgcgacagca cgtcgaccgc cgtggcgcag accttaacgc gggcccacag cttatcgttt
    16681 ccttcgacca ccgtccacgg cgcggtcgcg gtgctcgtct tgagcaacat gtcttcgacc
    16741 gcttcctgat aggccggcca cttgtcgcga ttgcgccagt cctcctgggt cagcttccac
    16801 gacttgtacg cgatccgttc ccgctcttcg aaccttcgga gctgctcctc gcggctgatg
    16861 tgaatccaga gcttcaggag gacggcgccg aactccacca gttgccgctc gaaggagttg
    16921 atctcgcggt aggcccggcg ccatgcctcc tccgtgcaga aaccttcgac gcgctcgacc
    16981 agcacccgcc cgtaccagct ccggtcgaag atcgaaattt gtccgctctc gggaaggcgc
    17041 cgccagaagc gatacaggta gtgatgggtt ttgtcctctc cctccggagc gctgattggg
    17101 aagacggagt agccgcgcgg atcgagccgc tcggtcagcc gcttgattgc gccgcccttg
    17161 ccggccgcgt cccagccctc gaacaccatc accaccggcc gcttctgccg gtatacctgg
    17221 tagccaagct cccgcaattg gatctgtcgc cgcgtgagct gcacgtcgta ggtctcccga
    17281 tcgattgact ggctcaggtc gacggtttcc agcatcacgg cctccccttg cgcgatttct
    17341 gcctgtttcc gaccttcgcg cgggatttac cgcccaccgg acggaccggg ggggcggcat
    17401 cgcccaagcg cgcttcaagc gcacggatga cggtctcgaa gatttttcgc cgggcgaacc
    17461 accgggacgt ggcttcaaca atggtccagg gtccatactc cgattccgtc cgcgccagca
    17521 tctcttcggc cgccttcagg tagttgtcgt acttccggtg gcgcgcccag tcctctttgg
    17581 taacgcgcca gctttcgagc gggtctttct cgagcgcctc gaagcgcttt ttttgttcct
    17641 ttctgcttat gtggagccag aacttgacga tgacggttcc gtcgtcggca agcatctgct
    17701 cgaactcgac aatgtcctga aacgcgctgc tggcctcgcg tttggagatc agctttccaa
    17761 cgcggtcctg gagcacgcgg cgataccagc tttggtcgaa gatggccatc tcgccgtaat
    17821 tcggcgtgcg tgaccagaac cgccacaacc acgggtggtt ctgctcgtaa gtgcgcggcg
    17881 gctggatcgg atagagcttg aagccgcgcg gatccagccg ctcggtgagc gtcgcgatcg
    17941 cggagccctt gccggaggcg tcccagccct cgaaaagcag aatcgaggcg attctgtggt
    18001 cccagcaggc tttttccagg tcgtagagcc gttgctggag cgcgggcagg acggaccggt
    18061 attcctcttt gctcaactta agccggagat ttactttttc gagcatggcc gttggtgggg
    18121 aacatcatag cggaacgtgg cctccggttc cagggcgctg aaaaacccgg ttggcaggtc
    18181 aggaaatcgt tgatccactg cgtagcgcag gactcctgtc ctgcgtcgga gcgcttaccc
    18241 ggcgggtttt cagcaccctc ccagggcacc ctccctcact tgcatcgcgg ccctcactgc
    18301 gatgtgatgg aacacatgac aaggtgtcag ttcctatccg ctcccgtgac tgcactgctc
    18361 gtgttgccgc tctttgcctc atccgctgga gccgcggagt ttttcgtgta tttcggcacg
    18421 tacacccgtg gcccaagcaa gggaatttac gtcagccgtc tcgacaccgc taccggcaag
    18481 ttgaccgaac cgaatctcgc cgccgagacc ccgaacccgt ccttcgtcgc gctacaccct
    18541 aaccgcaagt tcctgtatgc cgtgagcgag ggcggtaaag ccggcgccgt ttccgccttc
    18601 gctatcgacc ccaagaccgg caggctcgcc ttcctcaaca aggtctcctc ccagggcggc
    18661 ggaccctgcc acctgaacgt cgacaaaacg gggaagaacg tcgttgtggt gaactacggc
    18721 acaggcagca ctgccgtcct cccggtcgag ccggacggca agctccgcga ggcgtcgtcg
    18781 ttcatccagc acaaaggatc gagcgccgac aagagccgcc aggaagggcc gcacgcgcat
    18841 tcggtgaact tctccgcgga caaccgcttc gccatcgtgg cggaccttgg cctggatcaa
    18901 ctactcgtct acaagttcga cgccgccaaa ggaacgatcg cacccaacga tccgccctat
    18961 gccaccgtcg ctcccgggtc gggcccgcgg cacttcgcgt ttcaccccgg cggcaagttt
    19021 gcgtacgcca tcaacgagat gaagtgcacc gtaaccgcct tttcgtggga tgccaaacgc
    19081 ggcgtgctca aggagatcca gaccatcggc acgctgcctg ccggcttcac cggcgagaat
    19141 gacacggccg aggtgcaggt ccacccgaac ggt
//
LOCUS       DATIFQ010000274         2700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_856306, whole genome shotgun sequence.
ACCESSION   DATIFQ010000274 DATIFQ010000000
VERSION     DATIFQ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856306"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VLE22_12765"
     CDS             complement(<1..292)
                     /locus_tag="VLE22_12765"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HSB15324.1"
                     /translation="MTQQQGGIGIERLCELSRVARSGYYRYWKRAAPPEADVELRDRI
                     QRICVAHRFYGYRRVQSELGKQGFRVNHKKVRRLMREDNLLALRKRQWITTTD"
     gene            complement(289..651)
                     /locus_tag="VLE22_12770"
     CDS             complement(289..651)
                     /locus_tag="VLE22_12770"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB15325.1"
                     /translation="MKKNRVFSPAFKAAAVRRMQAGESPSALARALKVRRKLLYEWRE
                     AVLAGRPLRTGGRPRKQSTEASPPEEVADRIKQLEQLVGQLTLENRFFKGALRRIREL
                     RQQNGGAGAAASSRKSKP"
     gene            1026..1508
                     /locus_tag="VLE22_12775"
     CDS             1026..1508
                     /locus_tag="VLE22_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15326.1"
                     /translation="MQSFKIENFVRENPGLSPPRFQPLSDSEASALAERLLTRAGRPK
                     GTPSEVIRWLSEQATPLGGLNLNENEVALHELFRRAGIKPGPVVYVEWGPLRDVDRFE
                     TDELCRYFYDVWYPSADDVEIFDDTTAWLIFVRHYGGVEVWQAPLISGHIRDGSKAGV
                     "
     gene            complement(1833..2561)
                     /locus_tag="VLE22_12780"
     CDS             complement(1833..2561)
                     /locus_tag="VLE22_12780"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HSB15327.1"
                     /translation="MHRAWLLLLLTELAGFGQPTRPVYVIHRAPSPVTLDAKLDEPAW
                     RNAPEAGEFYFNWWKEGEKERTAARLLWDADNLYVSYRCQDRHISAYVTERHGPVSKD
                     DCVEIFLSPNISKARNYYTFEINAIGAMLNRCRTDWWTGPPTWEPEGVRYRTTFHGLP
                     KKDESPDDREWITELAIPFRNFARDAAHTPPEDGDRWRLNLMRTGGKTNAQHSTWSPI
                     PPPSETFHTPEAFGWVEFSKEPAR"
BASE COUNT          516 a          828 c          827 g          529 t
ORIGIN      
        1 tgtcggtggt ggtgatccac tggcgcttgc gcaacgccag caggttgtct tcccgcatta
       61 gccgtcggac ctttttgtga ttgacgcgga agccctgctt gcccaattcc gactgcaccc
      121 gccgatagcc gtaaaagcga tgcgccacac agatgcgttg gatccgatcg cgcaactcca
      181 catcggcttc cggaggggcc gcgcgcttcc aatagcggta gtagccggag cgcgccaccc
      241 ggctcagctc gcacagccgt tcgattccga ttccgccttg ctgctgcgtc atggcttgga
      301 tttgcgcgaa gacgccgccg cgccagctcc gccgttctgc tggcgtagct ccctgattcg
      361 ccgcaaggca cctttgaaaa aacggttctc cagcgtgagc tggccaacca gttgttccag
      421 ttgcttgatt cgatccgcga cttcctcggg cggagacgcc tcagtggact gtttcctggg
      481 ccgcccgccg gttcgtagtg gccgtccggc cagaaccgcc tcgcgccact catacagcag
      541 cttgcgccgc acctttaacg cgcgggccaa cgccgaagga ctctccccgg cctgcatccg
      601 gcgcactgct gctgccttga acgccggact gaaaactcgg ttcttcttca tcagtttcag
      661 ttctccattc actgtagaac tgtccctgat ttccgtgtct caccttaggg gcgcactcca
      721 ctgatccgct caggtttccc aaaacgcagc gctgtcttcg tgacataatc tgtgcttcgg
      781 atagggtagt ggcggatggc cgccaaaact cacagaaaca tgcgcagatc aggctgtccc
      841 cggttccccc ggttccccgg ttccaaaagg gctataggtt agatcccgga catccgaatg
      901 caaagcctgg aagccccgaa tctcaaacac acattaactg gtgggatttc accaaaggga
      961 agaagaacaa aggcggccgg acgggtgcgg agcccgttgg accgcgtaac caggagcagt
     1021 actagatgca gtcattcaag atcgagaatt tcgtacgaga gaatccgggc ctatctccgc
     1081 cgcggttcca gcccctgtcg gattcagaag cgagtgcgct cgcagaaaga ctgctcacca
     1141 gggcgggtcg gccaaagggc acaccatcgg aggtcatccg atggctttcg gaacaagcca
     1201 caccgctcgg cggcctgaac ctcaatgaaa acgaagttgc gctccatgag ttgttcagaa
     1261 gggccggaat caagcctggt cccgttgtgt atgtagagtg gggccccctg cgtgacgtcg
     1321 accgttttga aaccgacgaa ctttgcagat acttctatga tgtgtggtac ccaagtgccg
     1381 atgacgtcga gatatttgat gatacgacgg cgtggctgat attcgtgcgg cactacggag
     1441 gcgtcgaggt ctggcaggcg ccgctaatct cagggcacat tcgtgatgga agcaaggccg
     1501 gagtttaagg gacggtggaa aacccgtcag cctgcgcaaa tttcccggga gccgaaaacg
     1561 cgtcagaaac cccggtgaca gactggactg caccccaacc gcaccgcaaa acagagatgg
     1621 atccgcaatc actccattct gctgcctcga tctcctcgcg attcaacgga tcggcccgca
     1681 gagcaggggc cggttcaccg ttccggccct atgatgagac cgggagtcgg tactggcgag
     1741 ccgacccccg caaatggggg ggattggctt tgtttggtca aaactccggc gcgaacgaag
     1801 ccgcccaacg gaacctgcaa gggccggctt cgctaccgtg ccggctcctt cgaaaactcc
     1861 acccaaccga atgcctcggg cgtgtggaac gtctccgaag gcggcgggat cggcgaccac
     1921 gtgctgtgct gggcgttggt cttcccgccc gttcgcatca ggttcagccg ccagcggtcg
     1981 ccatcctcgg gcggcgtgtg ggcggcgtcg cgcgcgaagt tgcggaacgg gatggccaat
     2041 tccgtgatcc attcgcggtc gtcgggcgac tcgtccttct tcggcagacc gtggaacgtc
     2101 gtgcgatagc ggaccccttc gggttcccag gtcggcgggc cggtccacca gtcggtgcgg
     2161 cagcggttca gcatcgcgcc gatggcgttg atctcgaacg tgtagtagtt gcgggccttc
     2221 gagatgttgg ggctcaggaa aatctccacg caatcgtcct tcgaaaccgg cccgtgccgc
     2281 tcggtcacat aggcggagat gtgccggtcc tggcatcggt agctgacgta caggttgtcg
     2341 gcgtcccaga gcaggcgggc ggcggtccgc tccttctctc cctccttcca ccagttgaaa
     2401 tagaactccc ccgcttcggg agcgttccgc caggcgggtt cgtccagctt tgcgtcgagg
     2461 gtgacgggcg atggcgcccg gtggattacg tagacgggac gagtcggctg gccgaagccg
     2521 gccagttcgg tgagcaggag cagcagccac gcacgatgca tcaccccagc gtatcgcgga
     2581 gacgggccgc cgcaggagtt tcaggagttt cagggacagg ctacgtaatc gccaaatttg
     2641 aagtgtcagc gagaggagtt ccggagaagc cggggacggg ccgttctctg tcactcgcgt
//
LOCUS       DATIFQ010000275         7992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2581735, whole genome shotgun sequence.
ACCESSION   DATIFQ010000275 DATIFQ010000000
VERSION     DATIFQ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581735"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..542
                     /locus_tag="VLE22_12785"
     CDS             234..542
                     /locus_tag="VLE22_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HSB15328.1"
                     /translation="MAKKPARLDPIHPGEILSEEFMKPLGISINRLSRDLNVPPNRIH
                     GIVHGTRSITADTALRLGEYFGMSPETWMNLQAEYDLRTARRVVGDEITRTVRKRSAA
                     "
     gene            complement(589..1389)
                     /locus_tag="VLE22_12790"
     CDS             complement(589..1389)
                     /locus_tag="VLE22_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15329.1"
                     /translation="MRLSRAWLIPVCLVVPASVPARGQNLTSDERVDLIRGLTAEYAT
                     AKVAVPRSKKALVVDPKGGYDKKHWDVAGREFGPAARVGDIVQITKVELEDDRIVFQL
                     NGGMKGGRKWYDRIQVGAGGRTAPISSGSYSVAPSGTTVALVFPDRLPALNTVKVKEI
                     MAPVLDFNMRSATEHYVESLPPEIQTAIKEKKAREGMTRDQVLLALGRPKNKVRETKD
                     GNELEDWVYGDPPGRITFVTFDGDKVIRVKEAYAGLGGEVSPPLKPPL"
     gene            complement(1390..2244)
                     /locus_tag="VLE22_12795"
     CDS             complement(1390..2244)
                     /locus_tag="VLE22_12795"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HSB15330.1"
                     /translation="MAPGWQAHIIGVAVRVGAYALLVLGGLMLFSQLLAWFGSYVLAS
                     AAGVFLAAVVANALAMRIFERTHLAEIGLVWTPASVRNLLLGLAGGAGAALLVLAGPL
                     AVGAASFEAIPKGVFDWRTLLFISVALLFGAAGEEMMFRGYGFQALIGILGPVATVLA
                     VGVLFGLAHLSNLNISKVGVANTMLWGVLLGFAFLRSGDLWLPIGLHFGWNWILPLFG
                     VNLSGFTMDVTGYVMRWRIGSLWSGGNYGPEGGLLTTGVLVLLSLFLWKAPIIRQVPF
                     LLRPRAEE"
     gene            complement(2337..2852)
                     /locus_tag="VLE22_12800"
     CDS             complement(2337..2852)
                     /locus_tag="VLE22_12800"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSB15331.1"
                     /translation="MRGFLLVVTVLVALLAIFAVAMLPDRHVGSRSVHFSQPPEFIWH
                     AVADVENSPAWRPDLKEVSQLPDRDSHPVWRESYSDGSVIVLETSESVPMRRLVRRTV
                     ETSLPFASAWTVDIVPAGDGCTVTVTEDRRIRNAVVRFIGRFVNGRGPTVDRYIEALG
                     QRLGEPVHPLP"
     gene            2903..3412
                     /locus_tag="VLE22_12805"
     CDS             2903..3412
                     /locus_tag="VLE22_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSB15332.1"
                     /translation="MPARRILQLGDPLLRAVSRPVERPEEARLIIRDLRATLHEFQRT
                     HGFGRGISAVQIGETQRVIYLEFGGIPYAMVNPEFEWQSEDRFRLWDDCFSFPNLMVY
                     VERALAARVRYQDLSGAIRTIEGSGPLAELLQHEMDHLDGILAVDRAIGRESFCTREE
                     YERRHVGTR"
     gene            3515..3892
                     /locus_tag="VLE22_12810"
     CDS             3515..3892
                     /locus_tag="VLE22_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB15333.1"
                     /translation="MVDRRAETRMLCADLVKVRWTDRSGRNRKAVANLEDISLSGVCL
                     QMDTPLPIDTTLHIHHDKAEFKGSVRYCLFRDIGYFLGVQFAPGCRWSKRQYRPQHLL
                     DLRKLVQASAKRAAKKAPPMTIQ"
     gene            complement(3886..4857)
                     /locus_tag="VLE22_12815"
     CDS             complement(3886..4857)
                     /locus_tag="VLE22_12815"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HSB15334.1"
                     /translation="MEVTYLEAIRQGIWEEMERDPDVFIIGEDIGVYGGAFKVTAGML
                     DHFGERRVVDTPISESAIAGASIGAALMGLRPVAEMQFADFITCAYDQLVNFAAKCRY
                     RWGAAVPMVVRAPSGGGTHGGPFHSQNPEAWFAHVPGLKVVTPATAYDAKGLIKSAIR
                     DNDPVIFFEHKGLYRRIKGNIPDEEYTVPIGKASVFREGTDISVITYGAMVYVARDAA
                     DELAKEGISIEIVDLRTVFPLDEAAVLASVRKTSKAILLHEDILSGGVGGEIAARIAE
                     KAFDHLDGPVVRIAAPDTPVPFSPPLEQAFLPSVPKVIEKARWLYNY"
     gene            complement(4861..5901)
                     /locus_tag="VLE22_12820"
     CDS             complement(4861..5901)
                     /locus_tag="VLE22_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HSB15335.1"
                     /translation="MAAVKPIEPPIGVELSSNPDPNAELAFRFLHYLLMMREVEDRIE
                     RKLYRQGKILGGVYVGRGQEAIPVASGMVAEPDDVLFPSHRDMALYLIRGVHPRQIFA
                     QYMGRIGGLTRGKDGNMHMGDIKLKIVAIISAMAASVPVAAGAALALKYKGKKDIVFV
                     FFGDGATSRGDWHEGINLAAVQRLPVVLICNNNQYAYSTPLSKQMACENVADRASGYG
                     MPAEIVDGNDVFAIHESVRRAAAHARAGLGPYLVECKTFRMTGHSAHDPANYVPKHLW
                     EEWGRKDPILRLEKQMLDKGWASQGDLDRVRSDIRSEVDEAVEWAEKSPYPEPSELLD
                     NVYEKPQTDGVE"
     gene            6108..6809
                     /locus_tag="VLE22_12825"
     CDS             6108..6809
                     /locus_tag="VLE22_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB15336.1"
                     /translation="MPASSDLPAARAAAAPRANAQPDATALVQNLAHELRQPMSTIES
                     IAYYLSIVLPRENPRLSGQLQRLQEQIQQINSILDDTVYYLGAVPRYPRPIDLNDLVR
                     RCVDRVPVGAPVRMNLAEKLPAVRLDREQARHFFRSVISVFRRLAPGTEPVTISTSAT
                     GAVVQFEVYAAGAPLGPSERQDLWEPFAAALPPSAGLSMASAKRIAEAHGAVMDLEPG
                     ESGGLRLTVTFPAAT"
     gene            complement(6781..7359)
                     /locus_tag="VLE22_12830"
     CDS             complement(6781..7359)
                     /locus_tag="VLE22_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15337.1"
                     /translation="MLVVVGGHSRNVGKSAVVAGLIRALPEGHWTAIKITHHGHGAQE
                     SFSLSRNTVPDDSDTGRFLASGASQSWWLRAAPGSLPEALQKLRAVLAASGNAIVEST
                     SILDHLTPDLFLLVVNPSVMDWKDSALRFLERADALAIVTPHEGVSPQWQRLSDRVAG
                     KPRFPVSPPGYTSSALVAFVEERLRLLPGTSR"
     gene            complement(7373..>7992)
                     /locus_tag="VLE22_12835"
     CDS             complement(7373..>7992)
                     /locus_tag="VLE22_12835"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HSB15338.1"
                     /translation="VSLGERCVVHPNVTVYDDVDVGARAVLHSGCVIGADGFGFVLEG
                     DRYHKFPQVGRVWIGDDVEIGANSCVDRAALGVTWIGEGTKLDNMVHVAHNCRIGRHV
                     VVAAQTGFSGGVVVEDYAVIGGQVGIGDKARIESRAVLGSGCGVLTSKIVRAGQVVWG
                     TPARPLKEHLELLANLGRLPATRKELADLKHRLEALERKAGTGGA"
BASE COUNT         1518 a         2474 c         2589 g         1411 t
ORIGIN      
        1 ctaccacagt gctattcttc ttcatggccg gtctcctatg tgattgcacc tcgagtgcgc
       61 cacccattgg gagcttaacg catcccgccg ggagaccggc cttctcatcc catctcggta
      121 gcagacgttc gctcgtctcc agttcggtgt gcggccattc ctcttgacat aatacctgag
      181 ccgtcataag gttgggattg gacgtggaga gcgtcgatta tcactgagga cgcatggcga
      241 agaaaccggc aaggctcgat ccaatccatc cgggggagat tctgtccgaa gagttcatga
      301 agccgcttgg catcagcatc aaccggttgt cgcgcgactt gaacgttcct cccaaccgga
      361 tccatggaat cgttcacggg accaggtcga tcacagcgga cacggcgttg cgcctgggcg
      421 agtacttcgg aatgtcgccg gaaacctgga tgaacctgca agcagaatac gatctccgta
      481 ccgcccggcg ggtggtcgga gatgagatca cacgaaccgt ccgtaaacga agcgcggcgt
      541 agtgtcaggt ccgatgagac ggcggccttc ggggtaaagc cctgaaatct agagcggcgg
      601 tttcagcggg gggctgactt cgccgccgag gccagcgtag gcttccttga cacggatgac
      661 cttgtcaccg tcgaaggtga cgaaggtaat gcggccgggc gggtcgccat agacccagtc
      721 ctcgagctcg tttccatcct tggtctcacg gactttgttt ttcggacggc cgagcgcgag
      781 cagcacctgg tcccgggtca tgccctcgcg cgccttcttc tccttgatgg cggtctggat
      841 ttccggaggc agcgattcga cgtagtgctc ggtggcgctg cgcatgttga agtcgagcac
      901 gggcgccatg atctccttga ccttcacggt gttcagagcc ggcaggcgat ccgggaagac
      961 gagagcgact gtggtgccgg acggcgcaac cgaataggag ccgctgctaa tgggtgcggt
     1021 gcggccgccc gccccaacct gaatgcggtc gtaccatttc ctcccgccct tcatgcctcc
     1081 gttgagctgg aagacgatgc ggtcatcctc cagctcaact ttggtgatct gcacgatgtc
     1141 gccgacgcgg gcggccggtc cgaattcacg cccggccacg tcccagtgct tcttgtcgta
     1201 gccacccttc gggtcgacca cgagcgcctt tttggagcgg ggaacagcga ctttggcggt
     1261 ggcgtactcg gcggtaaggc cgcgtatcag atccacgcgc tcgtccgacg tcaggttttg
     1321 accccgggcc ggcacggacg ccggaaccac gaggcacacc gggatgagcc atgcccgcga
     1381 cagccgcatt cactcctccg cgcggggccg cagcaggaaa ggaacctgcc ggatgatggg
     1441 cgctttccac aagaagagag agaggagaac cagaacgccc gtcgtcagca ggccgccttc
     1501 gggaccgtag ttcccgccgc tccagaggga gccgattctc cagcgcatca cgtaccccgt
     1561 tacatccatt gtaaacccgc tcaggttcac cccgaagagc ggaagaatcc agttccatcc
     1621 gaagtggagg ccgatgggca gccacaggtc gccgctgcgg aggaaggcga agccgagcaa
     1681 cacgccccag agcatcgtgt tggccacgcc gactttcgaa atgttgaggt tcgacaaatg
     1741 cgccaggccg aacagcaccc ccacggccaa gaccgttgcg acgggtccga gaatgccgat
     1801 gagcgcctga aagccgtagc cgcggaacat catctcttcg ccggccgcgc cgaacagcaa
     1861 agccacgctg atgaagagga gggtgcgcca atcgaacaca cccttcggaa tcgcttcgaa
     1921 agaagccgcc cccaccgcca gcggaccggc gaggacgagc agcgccgcgc ccgcaccacc
     1981 ggccagtccg agcagcaggt tgcggacgga agccggcgtc cagacgagac cgatttcggc
     2041 aagatgggtg cgctcgaaga tccgcatggc gagcgcgttg gcgacgacgg cggcgaggaa
     2101 cacaccggct gcactcgcga gcacgtagct gccgaaccag gcgagcaact gcgagaacag
     2161 catcagcccg cctaggacga gcagcgcata agcgccgaca cgaacggcga cgccgattat
     2221 gtgcgcctgc cagccgggtg ccatcgggat tctcaggcct ccagaacctt gaccatagac
     2281 aaattgtccg cgaagcgaaa ggattactat agcgcggcgg tgcggaacgt gacacctcaa
     2341 gggagcgggt ggacaggctc gccgagtctc tgtccgagcg cctcgatgta acggtccacg
     2401 gtggggccgc ggccgttgac gaaacggccg ataaagcgca cgaccgcgtt gcggatccgg
     2461 cgatcttcgg tgacggtcac ggtgcagccg tcgccggcgg gtacgatgtc gaccgtccag
     2521 gcggatgcga acgggaggct cgtttcaacg gttcggcgga cgaggcgccg catcgggacg
     2581 gactcggacg tctcgagcac gatcacggag ccgtcggagt agctttcccg ccataccggg
     2641 tgactgtcgc gatcgggaag ctgggaaacc tctttcaggt ccggccgcca cgcaggggag
     2701 ttttcaacgt cggcgacggc gtgccagatg aactccggcg gctggctgaa atggacgctg
     2761 cgcgagccga cgtggcggtc tgggagcatc gccaccgcga aaatagccag taaggcaaca
     2821 agcacggtga cgacgaggag gaaaccccgc atgcacgacc tcccgcttga accaggttcg
     2881 aggtaaacat accataaacg caatgccggc gagaaggatt ttgcagctcg gcgacccgct
     2941 tctgcgagcc gtgtccaggc cggtggaacg accggaagaa gcgcggttga tcatccgcga
     3001 cctgcgcgcg acgctgcacg agtttcagcg aacacacggg ttcggccggg gcatcagcgc
     3061 ggtgcagata ggtgagacgc aacgggtgat ctatctggaa ttcggaggga tcccctacgc
     3121 gatggtcaac ccggagttcg agtggcagag cgaagaccgg ttccggctgt gggacgactg
     3181 tttttcgttc ccgaacctga tggtatacgt ggagcgggcg ctggcggccc gcgtgcgtta
     3241 ccaggacctg tcgggcgcca ttcggacgat tgagggaagc gggccgctgg cggaactgct
     3301 ccaacacgaa atggaccacc tcgacgggat tcttgcggtg gaccgcgcga tcggccgtga
     3361 gagtttctgt acccgcgagg aatacgaacg acgccatgtg gggacccgat gacagcggag
     3421 gattatcgct gagctggtac tcacggatac agggcggcgg taagtctttt tatcgcctaa
     3481 attctttcat ggagcggacc gatagatgag ttatatggtc gacaggcgag ccgagacccg
     3541 gatgctttgc gccgatcttg tgaaggtccg ctggacggac agatccgggc ggaaccgcaa
     3601 ggcggtcgcc aatctcgagg acatctctct ttccggcgtc tgcctgcaaa tggacacgcc
     3661 gcttccgatc gacacgacgc tgcatatcca tcacgacaag gccgaattca aagggagtgt
     3721 ccgctactgc ctgttccggg atatcggcta ttttctgggc gtacagtttg cgccggggtg
     3781 caggtggtcc aaacgccagt acaggccaca gcatctgctg gatcttcgca agctcgttca
     3841 ggcgagcgca aaacgcgccg cgaagaaggc gcccccgatg acgattcagt agttgtacag
     3901 ccagcgggcc ttctcgatca ccttcggcac actcggaagg aacgcctgtt ccagaggcgg
     3961 gctgaagggg accggggtgt ccggcgcggc aatgcgaacc accgggccgt caaggtgatc
     4021 gaaggccttc tcagcgatcc tggcggcaat ctctccaccc acgccaccgc tgaggatgtc
     4081 ctcatgcagg aggatcgcct tggaggtttt ccgcacgctc gcaaggacag ctgcttcatc
     4141 gagcgggaac acggtgcgta ggtcgacgat ctcgatcgaa atcccttcct tcgcgagctc
     4201 gtcagctgca tcccgggcca cgtaaaccat ggcgccgtaa gtgatgaccg aaatgtccgt
     4261 gccctcgcgg aacacggacg ccttgccgat gggaacggtg tactcttcgt ccgggatatt
     4321 tcccttgatg cgccggtaaa gccccttgtg ctcgaaaaag atcactgggt cgttgtcgcg
     4381 gatggcggac ttgatcaggc ccttggcgtc gtaggcggta gcgggagtca ccactttcag
     4441 gccgggcacg tgcgcgaacc acgcctcggg attttgagag tggaacgggc ccccgtgggt
     4501 tccgccgccg gagggagcgc ggacgaccat tggtacggcg gcgccccagc gatagcggca
     4561 cttggcggcg aaatttacga gctggtcata ggcgcacgtg atgaaatccg cgaactgcat
     4621 ctcggcgacg gggcggaggc ccatcagcgc ggcaccgatc gaagcgccgg cgatcgccga
     4681 ttcggagatc ggagtgtcga caacgcggcg ctcgccgaag tggtcgagca tgccggccgt
     4741 gaccttaaac gctcccccgt agacaccaat gtcctcacca atgatgaaga cgtctgggtc
     4801 cctctccatt tcctcccaga taccctggcg gatggcctcg agataagtga cttccatggg
     4861 ttactccacg ccgtccgtct gaggtttctc gtagacgttg tcgagcagtt cggagggttc
     4921 ggggtaaggg ctcttctcgg cccactccac cgcttcgtcc acctcactcc ggatgtccga
     4981 gcgaacacgg tcgagatccc cctgagatgc ccaccccttg tcgagcatct gcttctccag
     5041 acggaggatc gggtcttttc gcccccattc ctcccagagg tgcttcggca catagttcgc
     5101 ggggtcgtgg gccgagtggc cggtcatgcg gaaagtcttg cactcgacca agtaggggcc
     5161 gagccctgcc cgggcgtgcg ccgccgcccg gcgaacggat tcgtggatcg cgaagacgtc
     5221 gttgccgtcg acgatttcgg ccggcattcc ataaccggag gcgcggtcgg cgacgttctc
     5281 gcaagccatc tgcttcgaca ggggcgtgga atacgcgtac tggttgttgt tgcagatcag
     5341 gaccacggga agcctttgaa cggccgcgag gttgatcccc tcgtgccagt cgccgcggct
     5401 ggtggcgcca tcgccgaaga acacgaagac gatgtctttc ttgcccttgt acttcagagc
     5461 gagggcggcg ccggcggcta cgggcacgga tgcggccatg gcgctgatga tggcgacaat
     5521 ctttaacttg atgtcgccca tgtgcatgtt accgtccttg ccgcgggtga gaccgccgat
     5581 gcggcccatg tactgggcga aaatctgccg aggatggacg ccgcggatca ggtagagggc
     5641 catgtcgcgg tggctcggga acaggacgtc gtccggttcg gcgaccatgc cggatgcgac
     5701 cgggatcgcc tcctggcccc gcccgacata gacgccgccg aggatcttgc cctgacggta
     5761 gagctttcgc tcgatgcggt cttccacctc gcgcatcatc aggagatagt gcaggaagcg
     5821 aaatgcaagc tccgcgttcg gatcggggtt gcttgacaac tcgacaccga ttgggggttc
     5881 gattggtttc actgctgcca tgacagattc ccggtgcttg gagcgctggt ccaaagcctg
     5941 tagtatacgc caattcccgg cgctgtcacc ttcgagaggt gatcacgccc cggcgtgctt
     6001 gatggctgat ccttaagcgg ccttacattt ctgtttctta agccagccgg cgcacctcct
     6061 cccgatgcgc ggaagccgga agcggagata taatttcaac gccactcatg ccggcatctt
     6121 ccgaccttcc cgcagcacga gcagcggcgg cgcccagggc gaacgcacag ccggacgcaa
     6181 cggccttggt gcagaacctg gcgcacgaac tgcgccagcc gatgagcacg atcgagtcga
     6241 tcgcctacta tctgtcgatc gtcctgccgc gggagaatcc gcggctgagc ggccagttgc
     6301 agaggcttca ggagcagatt cagcagatca acagcatcct cgacgatacg gtctactacc
     6361 tgggcgccgt accccgctat ccgcgcccga ttgacctgaa tgatctcgtg cggcgctgcg
     6421 tggaccgagt gcccgtgggg gcgccggtcc ggatgaacct cgccgagaag ctgcccgcag
     6481 tccgcctgga ccgcgagcag gcgcgccact tctttcggag tgtgatctcg gtgttccggc
     6541 ggctggcgcc gggtaccgag ccggtgacca tttccacttc ggccactggg gcggtggttc
     6601 agttcgaggt gtacgcggct ggcgctccgt tggggccctc ggagcgacaa gatctgtggg
     6661 agcctttcgc cgcggcgttg ccgccgagcg ccgggttgtc catggccagc gcgaagcgga
     6721 tcgcggaggc gcacggcgcg gtgatggacc tggaaccggg cgagtcaggc gggctgcgcc
     6781 tcaccgtgac gttcccggca gcaacctgag ccgttcttcg acgaacgcta cgagagcgga
     6841 cgaggtgtaa ccgggtgggg agaccgggaa gcgcggcttc ccggccacgc ggtccgagag
     6901 acgctgccac tggggcgata caccttcgtg aggtgtaacg atggccagcg catccgcccg
     6961 ttcgaggaac cggagcgccg agtccttcca gtccataacc gaaggattta ccaccagaag
     7021 gaacagatca ggcgtgaggt gatcgagaat gctggtggat tcgacaatgg cgttcccgga
     7081 tgcggcgagg accgcacgga gtttctgcaa cgcttcgggc aaagaaccgg gcgcggcacg
     7141 cagccaccag gattgactcg caccggaggc gaggaaccgg ccggtgtccg agtcgtcggg
     7201 aacagtgttc ctggagagag agaaggactc ctgggcgccg tggccgtgat gagtgatctt
     7261 gatcgcggtc cagtgtccct cgggcaaggc ccggatcagg ccggcgacaa ccgccgactt
     7321 acccacgttc cggctgtgcc cgcccacgac tacgagcatg ggactcgtcc gctcaggcgc
     7381 ctcccgtccc ggccttcctt tcgagcgcct cgaggcggtg tttcagatcg gccagttcct
     7441 ttcgcgtcgc cgggagccgg ccgagattgg cgagcaactc gaggtgctcc ttgagcgggc
     7501 gagccggggt tccccacaca acctgacccg cccggacgat cttcgacgtg agcacgccgc
     7561 aacccgatcc gaggacggcg cgggactcga ttcgcgcttt gtcgccgatg ccgacctggc
     7621 cgccgatcac ggcgtagtcc tccaccacga cgccgccgga gaatccggtc tgtgccgcca
     7681 ccacgacgtg gcgtccgatg cggcagttgt gggccacgtg gaccatgttg tcgagcttgg
     7741 tcccttcgcc gatccaggtg acgccgagcg ccgcgcggtc gacgcaggaa ttggcgccaa
     7801 tttcgacgtc atcgccgatc cagacgcgcc cgacctgcgg aaacttatgg taacggtccc
     7861 cctcgagcac aaagccgaag ccatccgcgc cgatcacgca accggaatgg aggactgcgc
     7921 gggcgccaac gtctacgtcg tcatagacgg tgacgttcgg atgtacgacg cagcgctctc
     7981 cgaggctgac cc
//
LOCUS       DATIFQ010000276        27019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2196268, whole genome shotgun sequence.
ACCESSION   DATIFQ010000276 DATIFQ010000000
VERSION     DATIFQ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 27019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27019
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196268"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VLE22_12840"
     CDS             <1..568
                     /locus_tag="VLE22_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSB15339.1"
                     /translation="SDRAAYVLGEQWMGVAQGISDVIFLAVGTGIGAGILSGGRLIRG
                     AGDIAGSVGWFALDPRRKEGYARIGCMEAEAAGPALARRLGAPSAEAVIEAARSGDEA
                     ALAAVRETAMYLAMGIANLISAFNPRMVVLGGGLMQAGDLFLEPVRREVLEWAQPVSV
                     RQVRIELTKLGEDAGLLGAARLALFGAP"
     gene            596..1351
                     /locus_tag="VLE22_12845"
     CDS             596..1351
                     /locus_tag="VLE22_12845"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HSB15340.1"
                     /translation="MSAQKYYDQITGVLEKIRTTQMERIGKAAEAMASAIAAGNRVYL
                     FGSGHSVIPVQDIYPRYGSFVGFFPLYDPRLMWFNVVGPGGARELLWLERSEGYARVF
                     LQSYPLHAGDCMLVFSHGGLNAAPIEVALEAKAKGLTVISVSSHANAAVTEATHSTGK
                     KLSDIAAIAIDNCVPPEDALVDVGWKEKVAAGSTMAAVFISMALVAETGARLAARGVH
                     PPTFVSPNVAGVEKDHNLRVFDAFTQKLFERRP"
     gene            1348..2529
                     /locus_tag="VLE22_12850"
     CDS             1348..2529
                     /locus_tag="VLE22_12850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB15341.1"
                     /translation="MTRRAVSIPWLSAAAWAGMFFFGVVMAMLGTVLPVLSERLRFDL
                     ARAGGLFLAMNFAMLATTLVLGPLIDRFGKKPVLVAGSLAVSAALVLIAAAASYETLR
                     AAVFLLGAGGGALNGGTNTLIADLHANPARKSSALNVLGIFFGFGALFQPFLLGTLVQ
                     RLDLAPILYVTIGLSFVPLAMFAALPFPPPRVGPGFRLAEAGKLVRNPLVLALGFLLF
                     FQSGNEFIVGGYTSTYLNRDLNASIRTASYILAAYWGAMMLGRVISSRLVYRMKGSKL
                     LTASALLAAGGVAWMLLAGSAGTAAVGVILVGLGFASIYPVTLGFAGSRFEHISGTAF
                     GILFAIALTGGMTMPWLVGQLAAARGLRFAFWLAAANCLAITLLQFVVSRLGRRTGRA
                     E"
     gene            complement(2479..3204)
                     /locus_tag="VLE22_12855"
     CDS             complement(2479..3204)
                     /locus_tag="VLE22_12855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSB15342.1"
                     /translation="MISKAGLGSRTEACRWIGAGRVSVNGRVIRTPDHWVDLERDRVT
                     LDGKQVRAGDKIYLLLYKPKGYLTTYKDPEGRPTVYDLLEDVEEWVFPVGRLDLDTSG
                     LLLVTNDTQFAERITNPEYKVPKTYLVKTSTRLGDEQLDQLRNGVVLSDGPTRPAAVK
                     RLRDSGNRTFFEITITEGRNRQVRRMVEAAGSKVLKLVRTSIGPIEIDALPIGKHRRL
                     TAAEVGALLRAAGAPAKTRHHEL"
     gene            complement(3276..4595)
                     /locus_tag="VLE22_12860"
     CDS             complement(3276..4595)
                     /locus_tag="VLE22_12860"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15343.1"
                     /translation="MSDTRRAFLGKAGAAVVYSLSGAGSYLKAASAYDQIGLGFIGTG
                     IQGTILMGEFIEMPGVRPVIVADLYDGCLQRAKEQTGGVIETTKDYHKVLERKDVDAV
                     VIATPDHWHKRIVLDALAAGKHVYIEKPMTWSIDEGKEIIDAAAKSGKLLQVGSQGKT
                     SALTAKARELVKSGALGKVNMVRMSNNRNSAEGAWVYPIPPDASPQTVDWEQFLGNRP
                     KKPFDPKVFFRWRCWWDYSGGVATDLFVHLLTTLHEIMDVPGPASVVSNGGLYKWKDG
                     RNVPDVMNSVFEYEPGFVADMYVNLCNGRSGQPTTIMGTDATLVMDYGKMMLYPERVP
                     SEVQVYGSSAWPKAMRAAYFEAGGYTADGKPKTPPPEPKEAEEIKVERGPSHYEWFII
                     CLREGKPSREDATAGHYAAGAAHLANMSYRQGHRMRWDLNTGKVSQG"
     gene            complement(4656..6689)
                     /locus_tag="VLE22_12865"
     CDS             complement(4656..6689)
                     /locus_tag="VLE22_12865"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSB15344.1"
                     /translation="MTIPPAAGGSGTAPDSPVSTLIEKIHAEVNTEEAMRNMREVYES
                     DRFFTFPAFQKTAEQLAGKLKGIGLSRVELLHSPADGVTQYGFWTMPMAWDARGARLE
                     IVEPAVPAEQRVLADYPRIPTSLGMWSGSTPPEGITAEVVEMAGDSPEEIDKLDMKDK
                     LVLTRFNLLGSTTSSKWIFVKKGALGVINAFTENPELKDGRHWVNFWGDSGWAFTKKS
                     TPLICFSVSPRGSDLLHELLVRHGRVIVKAKADTRHYAGTYPYVTAVLPGLSNEEEVL
                     ELGHTSEQGANDNAAGVAAMVEAVATLKRLIEAGKLPRPRRSIRILAMGELYPTMHYL
                     ATYPERSRNTVAAWCVDTPAAPYELAGTEYSFHLNPDVGRSFIDALTVRIASEYFSKI
                     TPPRPFREASYGTGTDNFLSDPAIGVPTIWPFAGTGIPTHHNSEDTPERVDARSLRDI
                     TVVTAAFLYTVASADESELRWLAETAASRAAQVLQEDGRRMIDRVFAVTDAGELGRLL
                     HRGIERNEYLFGRESQAVRSVLRLVPEGRRAAGEASLAPVFERLRRLEKENAGRLRDS
                     VNVRAGQLGLQTAVEPVATPHPQAAAASRMVVKRKRMGTIPLDDLPVEQWEGYPYGSW
                     ALVPVTSLYWCDGRRDLAEVIRLTGLEHDTAGFDFVGYFRFLARHGYVDLIER"
     gene            complement(6785..7477)
                     /locus_tag="VLE22_12870"
     CDS             complement(6785..7477)
                     /locus_tag="VLE22_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HSB15345.1"
                     /translation="MRQNALNNLLSRYPAVAYVAPFVTFLLLLAAGQYLTPGRQFWYP
                     VRVLAASILLVLVSRRVLNFRVARSAGSVLLGVAVFVIWAAPDLIWPGYRSHWLFQNS
                     LTGVAHSSVHPVAREYGLLLVVHVAGSALLVPIIEELFWRAWLMRYAISREFERVPLG
                     AYSAESFWITAVLFASEHGPYWDVGLLAGVAYNWWMLHTRSLGDCILAHAVTNGLLAV
                     YVLATGRWEFWM"
     gene            complement(7491..9803)
                     /locus_tag="VLE22_12875"
     CDS             complement(7491..9803)
                     /locus_tag="VLE22_12875"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB15346.1"
                     /translation="MRWRTGFLILLLLVGATSCSRDPEVVKRKYVENGNRYFDRGKYK
                     EASIMYRSALKKDLRYGEAYYRLGLVQLKLNQPMAAVASLRRAVELQPDNEDAKTRLA
                     DLYLAAYLVGQRGRDQLKTEVKDLAGQLLRKTPNSFDGNRLLGHVEWRDGNLKGAIER
                     FRKADLAKPNSPNLVLALSQVLAADSQFDEAEKLAKSLIEKDPSFGAIYDSLYVQYAR
                     QNRLADAENLLKLRVEKNPNNSQYMIRLAGHYYVLGRRDDASNVFQRMIDNPREFPNA
                     RQEVGDFYLRFREFAKAVEQYEAGVKNAAKKEDQLVLKRRVADALIAWGKRPEASNVI
                     EEVLKENPKDEAAKAMRASLMLDTGSREQLQGVLNDLQAAVTRMPNNPVVRYNLGRAH
                     WAKGELDHARTQFREAVNLRPDYMAPRLALTQLHVNRQEWALALQTANETLAISPANL
                     TARLLRAVALRGTGNPEQARKELVEIAQRDPSARDAYFQLGRMDLSARNFKQAEENFQ
                     KCGQNAGNDLRCMVGLVETYAAQNQYDKAIQLLQETGRKMPDRSEITVTLANTYVRAA
                     KYDQAATIFQELISKDPKSSDYHIKLGETYRRMGKQDAAIQYYRKAKDLSPNDPMAYA
                     PLAVMLDVVGRKAEAADLYRQLLKLQPDNPLALNNLAYVLAETGGNLDEALTLAQRAR
                     QKLPQDLNVADTLGWIYIKKNLAGNAIDIYKELTAKNPDNPTFRFHMAMALVQKGDRP
                     QARRELQTALQNKPSKEEEGKIRELIAKIG"
     gene            9947..10621
                     /locus_tag="VLE22_12880"
     CDS             9947..10621
                     /locus_tag="VLE22_12880"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSB15347.1"
                     /translation="MERLAFKNADRAQLSLLSSIERRCLVFLAQRMPEWVQPDHLTLL
                     GILSMLGAGLAYWYAARNRTGLVLATLFLALNWFGDSLDGTLARVRNRQRPRYGFYVD
                     HVIDSFGALLLLAGLGLSGYMSPAVAAGLLVAFLILSIEVYLATYTIGTFHLSHWKFS
                     PTELRLLLMIGNAVLFYRPTVRLFGRSFLLFDVGGVVGIVGMTLMLFAAVARHTVFLY
                     REERIP"
     gene            10618..11067
                     /locus_tag="VLE22_12885"
     CDS             10618..11067
                     /locus_tag="VLE22_12885"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HSB15348.1"
                     /translation="MIRLNFVRWLKFNAVGAAGIVVQLGALAALTSGLGIGYLVATAL
                     AVETAVLHNFFWHERWTWGDRTQGRRRMGAVLDRLLRFNLSNGLVSILTNLVLMRLLV
                     GRLGLHYLPANMLAIAAGSLFNFLLSHLFVFRGARATPTAPPAPAAE"
     gene            complement(11036..12403)
                     /locus_tag="VLE22_12890"
     CDS             complement(11036..12403)
                     /locus_tag="VLE22_12890"
                     /inference="COORDINATES: protein motif:HMM:NF027224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HSB15349.1"
                     /translation="MGDGISRRAFLGGSAASLLHAEDRQKPFTINDDGAWSWFEDERA
                     IVHNGKLIAGSVAAGTHDPARRGNVEVAVFDLRRGARSLSTLHANLQYDDHNSPALLV
                     LPDRHILAAYAKHGPENRFYYRISERPDDAASWQSEQTFTPSESSRITYSNLHYLRNE
                     NGGRGRLYNFFRGLDGRNKPSYAYSDDHGRTWRTGNVFIDVPATFRHRPYVKYASNGA
                     DTVHIFYTDGHPRDFENSTYHIFYRAGKLHRSDGSVIRSLAEGLRRPEEGTRIFAGNA
                     ENVGWVSDIHLDRDGRPFAAYSVQKDPKETPVGQEGQDLRYRYARWDGKEWNDLEIAH
                     AGTRLYKGEDDYTGNIALDPEDPGTVYISTNADPETGAPLRSRTDGRRHWELYRGAKS
                     DGGGRWTWTALTRDSAVDNIRPIVPKWPGDGVALLWLRGTYRAYTDYDLEVVTLTLPR
                     GPAER"
     gene            12402..13895
                     /locus_tag="VLE22_12895"
     CDS             12402..13895
                     /locus_tag="VLE22_12895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HSB15350.1"
                     /translation="MTSLYITRHGCFRHGYCARHDILFSGVFSRHHRKVRVLFGLSDV
                     LLTALAFEAAYQTRLHLQLERVFFIVVPTKALLLGVTVLVWVALGLWLEVYDKLDAGH
                     PRVIVRDSLRQCVLGAVAVILFEFLLRLDLSRTFLALFAVYSWCFLCLFRLAAGKLVG
                     IIRREFGAPLFVLVVGLGERARRLGDSLERSEKYGVRLTGFLADEGGPAPDHVQLSRS
                     YPVYPIAELPLLLQRRVIDEVLFAVDSRRLAELEEVFLECDEEGVRTRVAVDFFPHVN
                     SRVHLEELGSAPLLTFTAVPHDEIRLLLKRLVDVTVSFAGLLVLFPLMLIVATLIRLT
                     SAGPAVFRQVRCGLNGRRFVLYKFRSMCENAEDLKSSLEHLSHRRTAFKIPNDPRLTL
                     IGRWLRKFSIDEWPQLWNVLRGDMSLVGPRPAVPEEVEQYKRWQRRRLRMRPGLTCLW
                     TLAGRDALDFESWMKMDMEYIDNWSLALDWRILLQTIPRVLTGKGAH"
     gene            13900..14514
                     /locus_tag="VLE22_12900"
     CDS             13900..14514
                     /locus_tag="VLE22_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSB15351.1"
                     /translation="MHLVPTQEEVVALLEQTGALRRGHFEYPNGLHSNEYLQVALAMR
                     YYQHAKVLSVGLSRLLRANPEIRAIIPELSIVAPATGGLPVAYGVCEALRAHQVYWAE
                     QEDDKQPLRFRQYLEQLPGEKVLLVDDILRTGKKLTALKALVEANGAEVVGLAVIVHQ
                     PTPKTPDFSPLPFYYLAKLDGLYYRDAGSCDLCRQNAPLAKVWI"
     gene            14563..15909
                     /locus_tag="VLE22_12905"
     CDS             14563..15909
                     /locus_tag="VLE22_12905"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HSB15352.1"
                     /translation="MARLAILILFAASALAADFKAGVARVDITPSTPIWLSGYASRKH
                     PSEGVLHPLWAKALAIEDSKRNRVVIVTTDLIGLPRPISELVAARVQKEYGLDRSQLL
                     LNSSHTHTGPLVKANLELMFDLTPDDKRTLEAYSQKLTDNLVSVAGAALGKLAPANIA
                     FGQGQAGFAINRREPAGGGMRIGVNTEGPVDHSVPVLKVTAADGSLMAVLFGYACHNT
                     TLTGEFYKISGDYAGFAQIEVEKANPGATAMFLMLCGADQNPNPRSSLELAQQHGNTL
                     AASVGAVLSSKVQRVKGPIRAAFQIVEPEFQPHTRETFERLLTASNPVQVRNAKMMLR
                     AYDDGRPVRRTPYPVQAIRFGKSLTLVALGGEVVVDYALRIKREYPKENIVVAGYSND
                     VMCYIPSRRILKEGGYEAVDSMIYYGQPGPFTEEVEDTIFGTIHQVMSRVGRKAPR"
     gene            15943..16674
                     /locus_tag="VLE22_12910"
     CDS             15943..16674
                     /locus_tag="VLE22_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HSB15353.1"
                     /translation="MWSHPPESLEVACGGVHVWRAALDVSPELLVRFSDHLAEDERSR
                     AARFVFPRDRDRFIVARSVLRDILGRYLVLHPADIRFSYGESGKPELAPDLSGSRLRF
                     NLAHSRDLALYAFTLGREVGIDVEFVRSELAKEEIAERFFSEREAAELRALPPSEQPA
                     AFFRCWTRKEAYIKARGRGFRIPLASFDVSLGQPARLLAVRDDPAEAARWILCDIPVG
                     RDCQAALAIEGENPEFFLLDWLSAT"
     gene            complement(16667..17623)
                     /locus_tag="VLE22_12915"
     CDS             complement(16667..17623)
                     /locus_tag="VLE22_12915"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB15354.1"
                     /translation="MAGESGSTRWSRRAWIGGMSVAGLAGAGTMLYRQAPSFWRQYVR
                     EMGREIARPPVRPRPSEWPDRGLYAAWLGHSTVLLKIDGFTILTDPVFSDRAGLNFGP
                     LTLGLKRLVAPALGIDNLPKIDLVLLSHAHMDHFDIPSLRHLEDKRTPVITAWETSDL
                     LRVDRYRKVTELRWDERTRVGPAEIRAFEVNHWGARMRSDTYRGYNGYLIESGRYRVL
                     FAGDSAITDKFRVLKSSRPIDLAVMPIGAYNPWIRYHCTPEEAWRMGNDAGAEFFIPV
                     HHQTFQLSREPLTEPIERFLEAAERSPERVVTQHVGQEWRFT"
     gene            complement(17613..18254)
                     /locus_tag="VLE22_12920"
     CDS             complement(17613..18254)
                     /locus_tag="VLE22_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HSB15355.1"
                     /translation="MSHADANQLQEPQAAGDAIVSPDTRRERRLPPGQTRTQKWPVLD
                     ASGPPRIDLKTWRLRIGGGVQKPIEWTWEQVLELPRVKVFADFHCVTRWSRLGNLWEG
                     VSTREILARAGGPTPDARYVLAHGYDGGWTTNLPLSDFLAEDALVAISHDGEPLSLEH
                     GGPARLVVPQLYAWKSAKWLAGIELMEKDRPGFWERNGYHMHGDPWTEERYGW"
     gene            complement(18271..20553)
                     /locus_tag="VLE22_12925"
     CDS             complement(18271..20553)
                     /locus_tag="VLE22_12925"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HSB15356.1"
                     /translation="MNRIQSLWHAILESPNGSTRRPVVDKHFESNVKGLYIIGDLAGA
                     PVVKLAMEQGHELALHISSKPGAHGRDADVFDLIVAGSGAAGLNCALEAQDHGLRVLV
                     LEKSKISNTIEEFPEGKWVYAEPDSSPPKGKLWLDGARKEELIDRWREIVRENKLDVR
                     TEEGVSHIQKRKDGDFDVQTPKRTYRARRVVLATGQRGNPRKLNAPGEDRERVYHRLY
                     GPKQYQDEDILVVGGGNSAVEAALTLAERNRVTLSYRGTEFSRLFKDNRRRLDEAVAA
                     GWLRVILNSNVKQFDERTCVLEIRKGPATSTRELPYDHAFVLIGAELPAKFLHSLGIR
                     LENDWNGSVFSAAVLVLATMVGLWVFGGKTGMPVFAGLTMAGGLFALAGLAGLVATGV
                     KGNRFAWLGLTFLMAYTIYGAKLGENLEFWPFRGWGYKALSFLGRPWSFWYTVLYTTV
                     ITVFGLQAMKRWGLDRKDRFQIWRYVSLLSFQWVFFFLIPEFLFRWAVEYQWVGSRLA
                     EDPTFAQQAWRSYGIVYAWPLFFYTFFGSPHQIWVVWGVVLSFVLIPILAVFHGKRYC
                     SWICGCGGLAETLGDRWRHLAPKGKTSIRWEFMNRVVFGAAAAVTLMVLLRDVVGVLR
                     APAEAGVLAYRLTADVWLVGILPVSLYPFFGGKIWCRYWCPLAKMMELFSSVFTRLRW
                     SRFAIHANEKCIACGECSRNCQVGIDVMDFALKQEELNNRNSTCIGCGICVTVCPMDV
                     LSFKPVAQPATSLVQISSIH"
     gene            20747..21850
                     /gene="gcvT"
                     /locus_tag="VLE22_12930"
     CDS             20747..21850
                     /gene="gcvT"
                     /locus_tag="VLE22_12930"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HSB15357.1"
                     /translation="MPEAPVLKRTSLHSLHRRLGAKMVDFGGWEMPVQYSGILDEHHA
                     VRRSVGVFDVSHMGEIEIRGPEALRLAEYVTTNAVSRLKTGQAQYSGLLYEHGGFVDD
                     VLVHKVEDDHYFFCVNASNQEKDFEHIRDRNRFNAAVDFASDRYAQIAVQGPKGLATV
                     QKLTSTDLSAIRYYYFTDGEVAGFPARIARTGYTGEDGVEVYVAPENAEALWDRMFDA
                     GQEFGVKPCGLGARNTLRLEAKMALYGHEIDASITPLEADLAWIVKMEKGDFIGRAAL
                     ERQQRDGVRRKLIGFEMRGRGIARDGYEVLVDDRPCGWVTSGGPSPTLNKNIGLCYLP
                     VELANVGLPIQVVVRNQPVDAETVPTPFYKRAK"
     gene            21847..22236
                     /gene="gcvH"
                     /locus_tag="VLE22_12935"
     CDS             21847..22236
                     /gene="gcvH"
                     /locus_tag="VLE22_12935"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HSB15358.1"
                     /translation="MTMYPDNLRYTKEHEWVEVEGDIGKVGITHHAQDQLGDIVYVDL
                     PKPGARVERGRSFGSVESVKAVSDVFSPVSGEVVEINETLGTAPEKLNQDPHGDAWLV
                     RIRMSAPDEIKQLMTAAQYQNYIGAEI"
     gene            22238..23554
                     /gene="gcvPA"
                     /locus_tag="VLE22_12940"
     CDS             22238..23554
                     /gene="gcvPA"
                     /locus_tag="VLE22_12940"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HSB15359.1"
                     /translation="MRYLPKSDSERREMLEACGVSSLDDFFSHLPPDVRLNRQLALPA
                     GQSEYEISAYFRARADQNATGYASFLGAGVYNHYRPVLVDTVVSRGEFLTSYTPYQAE
                     ISQGTLTTIFEFQTMICQLTGMEVANASMYCGSTAVPEAGMMAARITGRNRLLVARTL
                     HPEYREVLKTYARYQGMAVEEFGYTAGHGTVDLDDLDRRLDDKTAAVILQSPNFFGIV
                     ESVDAAADLAHKRGAMLIDVFTEAVSLGLLEPPRQADIIAGELQSFAISPSYGGPYAG
                     VIATREKYIRQMPGRLAGETKDSHGNRAYCLTLATREQHIRREKATSNICTNQALIAL
                     MATVFMTVYGKQGVRELAEQNLAKAHYLASRLPRKFAGPFFNEFVAAFPGKSPETING
                     MMLEKKIIGGLPLGRYYPELEDCMLLCATEMSTREQMDVFAEVLAQ"
     gene            23551..25032
                     /gene="gcvPB"
                     /locus_tag="VLE22_12945"
     CDS             23551..25032
                     /gene="gcvPB"
                     /locus_tag="VLE22_12945"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HSB15360.1"
                     /translation="MISKVRSHVTQNEPLLFELSSPGKKAYQLPELDVPPVDASKALG
                     AEHVRREVEGFPEVSEVEAIRHFTRLSTWNYAIDLGLYPLGSCTMKYNPRLNEAVARI
                     EGLAWAHPYQPESLSQGAMEIMSALESALIEITGMDAVTLQPAAGAHGELTGILLVRA
                     YLESLGNPRKKILIPDSAHGTNPATAAIAGYAVENIKSNDRGMIDLELLARVVDEDVA
                     ALMITNPNTLGVFEEQIARIAEIVHSKGAQVYMDGANMNALVGITRPGDFGMDVMHLN
                     LHKTFSTPHGGGGPGSGPVAVKKHLEPFLPLPRLTAGHAGLRFDYDRPRAIGRVKAFY
                     GNFGVIVRALAYIMAHGGEGLRNATMDAVLNANYIRKKLEGYYELPHRAPSMHECVFS
                     DARQAKQGVKTMDIAKRLIDYGFHPYTVSFPLIVHGALMIEPTETESKRELDLFVAAL
                     IAIAKEVEEDPEIVLKAPYGTRTGRVDEVTAARKPVLRWKPAD"
     gene            25053..26981
                     /locus_tag="VLE22_12950"
     CDS             25053..26981
                     /locus_tag="VLE22_12950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB15361.1"
                     /translation="MTRPLLFVLAVALLIRLPFLNQAIQGDDVYYLAGAQHAQIDPLH
                     PNHARYAYLGDVVDMRGHPHPPLNAWFLAGLLAMFGDIGEVPFHAAYILFTIVAALAM
                     WSLARRFSPRPLAATLLFLATPAFVVNGNSLESDIPFLAFWMASIALFAGALETRSRL
                     RLLASVGAMALAALAAYQSVVMVPILALHLWWNARNWRPAWAAVLASPVTLVIWQIWE
                     RASTGEIPVAVLGDYFQTYGFQALLPKLRSTVALTAHSAWIVFPVLALVAFRRAGRTA
                     WIAALAGAAGAAFLDPHPLFWASFGTGVLVLAWCAGRLRSASMEERFLAAWVVLFFAG
                     ALVLFFAGSARYLLPMAAPLALLASRALAHRPSWLTAAFVAQLALGLALSAVNFQHWD
                     GYRAFARELQHETETRRVWINGEWGLRFYFEADGGLPLMRGQAVQPGEMVVSSELAFP
                     IRFTTGGGVLVPVKEHEIRPSLPLRLFGLGARSAYSSASLGFRPFDFSTGPVDRVRAD
                     LVVERKPSLSYLPMNASEAPQQIVSGIYELEGGQWRWMGDRAVLLLRRPEAALPLHVA
                     LHIPSQSPARRITVTVDGVQVADKTFAAPGTYTLTSEPFVTASDSPTVTITVNRTFSV
                     AGDHRKLGVILSAVGFER"
BASE COUNT         4776 a         8807 c         8035 g         5401 t
ORIGIN      
        1 tagcgaccgc gccgcgtacg tcctcggcga acagtggatg ggcgtggccc agggcatctc
       61 ggacgtgatc ttcctggcgg tcggcaccgg catcggtgcc ggcatcttga gcggcggccg
      121 gctgatccgc ggagcggggg acatcgccgg atcggtcggc tggtttgcac tggatccgcg
      181 ccggaaggaa ggctacgccc gcatcggctg catggaggcc gaagccgccg gcccggccct
      241 cgccaggcgt ttaggggcgc cgtcggccga ggcggtcatc gaagcagcca ggagcggtga
      301 cgaagcagcg ctggccgctg ttcgcgaaac ggcgatgtac cttgccatgg gcatcgccaa
      361 cctgatcagc gcgtttaatc cccggatggt cgtactcggc ggcggcctga tgcaggcggg
      421 tgacctcttt ctggagcctg tccggaggga agtgcttgaa tgggcacagc cggtctcggt
      481 gcgtcaggtg cgcatcgaac tgacaaaact cggcgaagac gccggcctcc tcggagcggc
      541 tcgcctggcg ctgttcggcg cgccgtagag aatccgcacc gcttaccata agatcatgtc
      601 cgcgcagaaa tactacgatc agatcaccgg agttctcgag aaaattcgta cgacgcagat
      661 ggagcgtatc ggcaaagcgg ccgaggcgat ggcctccgcg atcgctgccg ggaatcgcgt
      721 ctacctgttc ggcagcggcc actccgtcat cccggtgcag gacatttacc cgcgttacgg
      781 cagcttcgtc ggtttctttc cgctctacga tccgcggctg atgtggttca acgtcgtcgg
      841 accgggcggc gcgcgagaac tgctgtggct cgagcgctcc gaaggctacg cccgcgtgtt
      901 cctccagagc tatccgctcc acgccggcga ctgtatgctc gtcttttctc acggcggtct
      961 gaacgccgcg ccgatcgagg tcgcattgga ggcgaaggcg aagggactga ccgtaatctc
     1021 ggtctcgtcg cacgccaacg ccgccgtcac cgaggcgacc cactctaccg gcaagaagct
     1081 ctccgacatc gccgccatcg ccatcgacaa ctgcgtgcct ccggaggacg cgctcgtcga
     1141 tgtcggctgg aaggagaaag tcgcagccgg ttccaccatg gccgcggttt tcatttcgat
     1201 ggcactcgtg gccgagactg gcgctcgtct cgccgcccgc ggcgtccatc cgccgacgtt
     1261 cgtttcccca aacgttgccg gcgtggagaa ggaccacaac ctgagggtgt tcgacgcctt
     1321 cacgcagaag ctgttcgaac gccggccgtg acacgccgag ccgtctcaat tccgtggctc
     1381 agcgccgccg cctgggccgg catgttcttc ttcggtgtcg tgatggcgat gctggggacg
     1441 gtcttgcccg tgctctccga gcgcctgcgc ttcgacctcg cgcgcgcagg cggcctgttt
     1501 ctggcgatga acttcgccat gctcgcgacg acgctggtcc tcggcccgct gatagatcgc
     1561 ttcggcaaga aaccagtcct ggtggccggc tcgctcgcgg tttctgccgc gctggtgctg
     1621 atcgcggctg ccgccagcta cgagacactg cgcgccgccg tcttcctcct cggggcgggc
     1681 gggggtgcgc ttaacggcgg gacgaacacg ctcatcgccg atctccacgc aaacccggct
     1741 cgcaagagtt cggcgctcaa cgttctgggg atcttcttcg gcttcggcgc gctgttccaa
     1801 ccgtttctgt tggggacgct ggtgcagagg ctggaccttg cgccgattct ctatgtgacg
     1861 atcggcctca gcttcgtgcc actggcgatg ttcgctgcgc tgccgttccc tcccccgcgg
     1921 gtcggaccgg gctttcgtct cgccgaggcc ggcaaactcg tccgcaatcc gctcgtcctc
     1981 gctctcggct tcctgctctt ctttcaatcg ggcaatgaat tcatcgtcgg gggctacacc
     2041 tctacgtacc tcaaccgtga tctgaacgct tcgatccgca ccgcgtccta catcctcgcc
     2101 gcgtattggg gcgccatgat gctgggccgc gtgatctcga gccgtctggt gtaccggatg
     2161 aaggggagca agcttctgac ggccagcgcc ctgcttgcgg ccggcggcgt cgcctggatg
     2221 ctgcttgctg gaagcgccgg cacggctgcg gtgggcgtaa ttctcgtcgg gctcggtttc
     2281 gccagcatct accccgtgac gctcggcttc gccggcagcc gttttgagca catctctggg
     2341 acggcgttcg gaatcctgtt cgccatcgcc ctcaccggtg ggatgacgat gccctggttg
     2401 gtgggacagc tcgccgccgc gcgcggtctg cgttttgcct tctggctcgc ggccgcgaac
     2461 tgcctcgcaa tcaccctgtt acagttcgtg gtgtcgcgtc ttggccggcg caccggccgc
     2521 gcggagtaac gccccgactt ccgccgcggt caatcggcgg tgcttaccaa tcggcagagc
     2581 gtcgatttcg atcggcccga tcgacgtccg gaccagtttc agcaccttgc tcccagcggc
     2641 ttccaccatc cgccgcacct ggcggttccg tccctcggtg atggtgattt cgaaaaacgt
     2701 cctgttgccg gaatcgcgca gcctcttaac cgcggccggc cgcgtcggac cgtcgctcag
     2761 gaccacgccg ttgcgcagtt ggtcgagttg ctcgtcgcca agacgagtgg aggtcttgac
     2821 caggtacgtc ttcggaacct tgtactcggg attcgtgatg cgctcggcga actgggtgtc
     2881 gttggtgacc agcagcaagc cgctggtgtc gaggtcgagc cgccccacgg gaaaaaccca
     2941 ctcctcgacg tcctcgagga ggtcatagac ggtgggccgt ccttccgggt ctttgtaggt
     3001 ggtcaggtag cctttgggct tgtaaagcaa caggtagatc ttgtcgccgg cccggacctg
     3061 tttgccgtcg agcgtgaccc ggtcgcgttc cagatcgacc cagtggtccg gcgtgcggat
     3121 gacacgaccg ttcacggaaa ctcgtcccgc gccgatccag cggcacgcct cagtccgcga
     3181 cccgagaccc gccttcgaga tcacgcgctc aagcgtcttc agtggacgct gggcgcgggc
     3241 gtcattcttc ttctttccgg ttcgattccc tgctctcagc cttggctcac cttcccggta
     3301 ttgaggtccc accgcatccg gtggccttga cggtatgaca tgttggccag atgcgcggcg
     3361 cctgctgcgt agtgccccgc cgtcgcatcc tcccggctcg gcttcccttc gcgcaggcag
     3421 atgatgaacc actcgtagtg cgacggcccc cgttcgacct tgatctcctc ggcttccttc
     3481 ggctctggag gaggcgtctt cggcttgccg tccgcagtgt acccgcccgc ttcaaaataa
     3541 gccgcccgca tcgccttcgg ccacgccgac gagccgtaca cttgcacttc ggacggcacg
     3601 cgctccgggt agagcatcat ttttccgtag tccatcacga gagtcgcatc ggtgcccatg
     3661 atggtcgtcg gctggcccga gcgcccgttg cacagattga cgtacatgtc cgccacgaac
     3721 ccgggctcgt actcgaaaac agagttcatc acgtcgggca cattacgccc atccttccac
     3781 ttgtacagcc cgccgttgga taccaccgag gctggcccgg gcacgtccat gatctcgtgc
     3841 aaagtcgtca gaaggtgtac aaacaggtca gtcgccacgc ctcccgagta gtcccaccag
     3901 cagcgccacc ggaagaaaac cttcgggtca aacggcttct tcggccggtt gcctaagaac
     3961 tgctcccagt cgaccgtctg cggcgaggca tccggcggaa tcgggtacac ccaggctccc
     4021 tcggcgctgt tgcggttgtt cgacatccgc accatgttca ccttgccgag cgcccccgac
     4081 ttcacgagct cgcgggcttt cgccgtcagc gccgacgtct taccctggct gccgacctga
     4141 agcagtttgc cgctcttcgc cgccgcatcg atgatctctt tgccctcatc gatgctccag
     4201 gtcatcggtt tctcgatgta gacgtgttta cccgcggcca gtgcgtcgag cactatccgc
     4261 ttgtgccagt ggtccggcgt cgcgatcacc actgcgtcca cgtctttgcg ctcgagtact
     4321 ttgtggtaat ccttcgtcgt ttcgatgacg ccgccggtct gctccttggc ccgctgtagg
     4381 cagccatcat acaggtctgc cacgatcacc ggccgcacac cgggcatttc gataaactca
     4441 cccatcagga tcgttccctg gatacccgtg ccgataaaac cgaggccgat ttgatcgtac
     4501 gcgctcgcag ccttgagata ggagcctgcg ccgctcaacg agtacacgac tgccgcgccc
     4561 gctttgccga gaaatgctcg cctggtatct gacatgaatc cgtgtgcctc cattctcaaa
     4621 attacaccat cggacctgat tagcggcaat gtgaattacc gctcgatcag atcaacgtag
     4681 ccgtgccggg cgagaaaccg gaagtacccg acgaaatcga agccggccgt gtcgtgttcg
     4741 agaccggtca aacggatcac ctctgcgagg tcccgccggc cgtcgcacca atacagcgat
     4801 gtaaccggga ccaacgccca actgccgtac gggtagccct cccactgttc aacgggaaga
     4861 tcgtccaagg gaattgtgcc cattcgcttt cgcttcacca ccatgcgcga cgcagccgcc
     4921 gcttgcggat gcggcgtggc caccggctcc accgcggtct ggaggccgag ttgccctgct
     4981 cggacgttca cggaatcccg gaggcgcccg gcattctcct tttcgagccg cctaagccgt
     5041 tcgaaaacgg gtgcgaggga agcctctccg gcagcacgcc taccctccgg aaccaggcga
     5101 agcacggagc gcaccgcctg tgattcccgg ccgaacaggt actcgttccg ctcgattcca
     5161 cgatgcagga ggcggccgag ttcaccggcg tcagtaacgg cgaacacccg gtctatcatg
     5221 cggcgtccgt cctcttgcaa cacctgtgcg gcgcggctcg ccgcggtttc ggccagccac
     5281 cgcagctcac tttcatccgc cgaagccacc gtataaagaa acgcggccgt cacgaccgtg
     5341 atgtcccgca gggatcgtgc gtccaccctt tcgggcgtgt cttcgctgtt atggtgcgtt
     5401 ggaatgcccg tcccggcgaa cggccagatc gttggaactc cgatcgccgg gtctgacaga
     5461 aaattgtcgg ttccggtacc gtacgacgcc tcccggaatg gccgtggcgg tgtgatcttc
     5521 gagaagtact cgctcgcgat ccgaacggtg agcgcgtcga taaaggatcg gccgacatcc
     5581 ggatttagat ggaatgaata ctccgtgccg gcaagttcgt acggcgcggc aggtgtgtcc
     5641 acacaccacg cggcgactgt attgcgggat ctttccggat aggtcgccag gtagtgcatg
     5701 gtcgggtaca gctcgcccat cgcgagaatg cggatgctcc gccgcggacg cggcagctta
     5761 ccagcttcga tgaggcgctt cagtgtggcc accgcttcga ccattgccgc tacgcccgcg
     5821 gcgttgtcgt tcgcaccctg ctccgatgtg tgcccaagtt ccagcacctc ctcctcgtta
     5881 ctcaatcccg gcagcaccgc ggtgacgtac ggatacgttc cggcgtaatg gcgcgtatcc
     5941 gccttcgcct tcacgatcac cctgccatgt cgtaccagca gctcatgaag aaggtccgac
     6001 ccccgcggcg acaccgagaa gcagatcagc ggcgtgcttt tttttgtaaa ggcccagccg
     6061 ctgtcgcccc agaaattgac ccagtgccgc ccgtccttca gctcgggatt ctcggtaaag
     6121 gcgttgatca cgccgagcgc acccttcttg acgaagatcc actttgagct cgtcgtcgag
     6181 ccgagcaggt tgaatcgggt gagcacgagc ttgtccttca tgtcgagctt gtcgatttcc
     6241 tcgggagagt cgccggccat ctcgacgacc tcggctgtga ttccttccgg cggtgtcgat
     6301 ccgctccaca tcccaagcga tgtgggaatg cggggatagt ccgccagcac gcgctgttcg
     6361 gcggggacgg cgggttccac gatctcgagc cgcgctcccc ttgcatccca cgccatcggc
     6421 atcgtccaga atccgtattg cgtcacgccg tcggcaggag aatgaagcag ttcgacacgg
     6481 ctcaggccga tcccctttag ttttccggcg agttgctccg ccgtcttctg gaaagcggga
     6541 aatgtaaaga accggtcaga ctcgtagacc tcccgcatgt tccgcatcgc ctcctcggtg
     6601 ttcacctcag cgtgtatttt ctcgatcaat gtcgacacag gtgagtcggg ggcagtaccg
     6661 gaaccacccg ccgcgggagg aatggtcatc gattgcatcg caagaacccc tgctaaaatc
     6721 caacacgttt gcacagtcat cgtcgccttc tccttaaagg gccgcacgaa tcaatgcctg
     6781 aaaatcacat ccagaactcc cagcggccgg tcgccagaac gtacaccgcc agcagtccgt
     6841 tcgttacggc atgcgccagg atacagtcac cgaggctgcg cgtgtgtagc atccaccagt
     6901 tgtaagcgac gcctgccaat agtcccacat cccaatacgg gccatgttcg gaggcgaaca
     6961 ggaccgccgt gatccagaac gactctgccg agtacgcacc caacggaacg cgctcgaact
     7021 cgcgtgagat cgcgtagcgc atcagccacg cgcgccagaa cagctcttcg atgatcggca
     7081 ccagcagtgc cgagccagcc acgtggacca ccagcaacag cccgtactcg cgggcaacgg
     7141 ggtgaacgga actgtgcgcc acgccagtga ggctgttctg aaacagccag tgtgaacggt
     7201 atcccggcca aatcaagtcc ggagcggccc agatcacgaa tacggccact cccagcagta
     7261 cgctgcccgc cgaccgcgca acacgaaagt tcagcacgcg ccgggagacg agaaccagta
     7321 atatcgatgc cgccagaacg cgtaccggat accagaattg gcgcccggga gtcagatatt
     7381 gtcccgccgc tagcagcagc aggaacgtca cgaacggtgc cacataggcc accgccgggt
     7441 agcgactcaa caagttgttc agggcgttct gccgcacccc aaacccggcc tcacccgatc
     7501 ttggcaatca gttccctgat cttgccctcc tcttccttcg aaggcttgtt ttgcagcgct
     7561 gtttggagtt cgcgcctggc ctgcggcctg tcgccctttt ggacgagggc catcgccatg
     7621 tggaatcgga aggtcgggtt gtccgggttc ttggccgtca gttccttgta gatatcgata
     7681 gcgttcccag ccaggttctt cttgatgtag atccatccaa gagtatcggc cacgttcagg
     7741 tcttggggca gcttctgccg cgcgcgttgc gccagtgtca gcgcctcatc gagattgccg
     7801 cccgtctccg ccagcacgta cgccaggttg ttgagcgcca gcggattgtc gggctgcagc
     7861 tttagtaact gccggtaaag atcggcagcc tcggctttcc ggccgaccac gtccagcatc
     7921 accgccagcg gtgcgtaagc catcggatcg ttcggactca gatccttcgc cttccggtag
     7981 tactgaatag cggcatcctg tttccccatc cgtcggtacg tttcgccgag cttgatgtga
     8041 tagtcactcg acttcggatc cttcgaaatc aactcctgga agatcgtcgc cgcctggtca
     8101 tacttcgccg cgcggacata cgtgttcgcc aatgtcaccg tgatttcgga acggtccggc
     8161 attttccggc ctgtttcctg gagtagttgg atggccttgt cgtactggtt ctgcgcggcg
     8221 tatgtctcca ccagacccac catgcatcgc aggtcgtttc cggcgttctg cccgcacttc
     8281 tggaagtttt cctccgcctg cttgaagttc cgggcgctca ggtccattcg cccgagctga
     8341 aaataggcgt cacgtgcgct ggggtcgcgt tgcgcgatct ccacgagttc cttccgcgcc
     8401 tgttcggggt tgcccgtccc gcgtagcgcc acggcacgca acagtcgcgc cgtgaggttc
     8461 gccggagaga tcgccagcgt ttcattcgcg gtctgtagcg ccagtgccca ctcttggcgg
     8521 ttgacgtgca actgggtcag cgccagcctg ggagccatat aatccggcct gagattgacc
     8581 gcctcacgga actgcgtcct ggcatggtcg agctcgccct tcgcccaatg cgcgcgcccg
     8641 aggttgtaac gcaccaccgg attgttcggc atccgcgtca ccgccgcctg caggtcgttc
     8701 aacaccccct gcagttgctc ccgtgagccc gtgtcgagca tgagcgaggc gcgcatggct
     8761 ttggccgctt cgtccttcgg attctccttg aggacctcct caatcacgtt cgacgcctcg
     8821 ggtctcttgc cccacgcgat caatgcatcc gcgacgcgcc tcttcagaac gagctggtcc
     8881 tccttcttgg cggcgttctt tacgccggcc tcgtattgct cgacagcctt ggcgaactcc
     8941 cgaaacctaa ggtagaaatc cccaacctcc tgccgcgcgt tcgggaactc ccgcgggttg
     9001 tcgatcatgc gttgaaacac gttgctggcg tcgtcgcgcc gtccaagaac gtagtaatgg
     9061 cccgccagcc gaatcatgta ctgggaattg tttggattct tctccacgcg gagcttcagc
     9121 aggttttcgg cgtcagcgag acggttctgg cgggcgtatt ggacgtataa ggagtcgtaa
     9181 attgcaccga aagacggatc cttttcgatc agactcttgg ccagcttctc cgcctcgtcg
     9241 aactgcgagt cggcggccag cacctgcgaa agtgccagca cgaggttcgg ggaattcggc
     9301 ttagccaagt ccgctttgcg gaagcgctcg atggctccct tcagatttcc gtcgcgccat
     9361 tcgacgtgtc ccagaagccg gtttccgtcg aaggaattcg gggtcttcct gagcaactgc
     9421 ccggcgaggt ccttgacctc ggttttgagt tgatcccggc ccctttgccc gaccaggtat
     9481 gcggccaagt agaggtcagc cagccgggtc ttggcgtcct cgttgtccgg ttggagttcc
     9541 accgcccgcc ggagcgaagc taccgctgcc atcggctggt tcagcttcaa ctgcaccagc
     9601 ccgagccggt agtacgcctc gccgtagcgc aggtccttct tcagtgcact gcgatacatg
     9661 atcgaggctt ccttatattt gcctcgatcg aaataccggt tcccgttctc gacgtatttg
     9721 cgcttgacta cctcggggtc acgactgcac gacgtcgctc ccacgagcag aagcagaatt
     9781 aagaatccag ttcgccacct catagatatt gccgccggtt ccgggttatt gtactcagac
     9841 atgatagaac acttctggag ttggcactcg aattgcccag tattcctagg accgggtgga
     9901 agccgtcggt gctgcgccgg cattaggctc ggatgaatgg attgaaatgg aacggcttgc
     9961 ctttaagaat gcggatcgtg cacagctcag cctgctgtcg tcaatagagc gcaggtgtct
    10021 tgtatttctt gcgcaacgga tgccggaatg ggtacaaccc gaccatctga cgctgctcgg
    10081 catactgtcc atgctcggag cgggactcgc ctactggtac gccgcgcgga accgcaccgg
    10141 tctcgtcctg gcaaccctct tcctcgctct gaactggttc ggcgacagcc tcgacggcac
    10201 gctggcccgg gtccggaacc gccagcggcc ccgctacggt ttttacgtgg atcacgtcat
    10261 cgattccttt ggcgctctac tcctgctcgc cggtctcggg ctctccggct acatgagtcc
    10321 tgccgttgcc gccggactcc tggttgcttt cctgattctt tcgatcgagg tgtacctcgc
    10381 cacgtacacg atcgggacgt tccatttgtc ccattggaag ttcagtccga cggagctgcg
    10441 gctactcctg atgataggca atgctgtcct tttctataga ccgacagtac ggctgttcgg
    10501 caggagtttc ctgttgttcg acgtcggtgg cgtggtcggc atcgtgggaa tgaccctgat
    10561 gctcttcgcc gccgtggccc ggcacacggt gttcctttac cgggaggagc gcatcccgtg
    10621 atcaggctga acttcgttcg ctggctgaaa ttcaacgccg tcggtgcggc gggtatcgtc
    10681 gtgcagttgg gtgcactcgc ggcgctcacg agcggcttgg gaatcggata tctcgtcgcc
    10741 accgcgctcg ccgtcgagac ggcggtcctg cacaacttct tctggcacga gcgctggacc
    10801 tggggggacc gtacgcaggg ccggcgtcga atgggtgcgg tcctcgacag actgctgcgc
    10861 ttcaacctga gcaacggcct tgtctcgatc ctgacgaacc tcgtgctgat gagactgctc
    10921 gtcggccggc tcggcctgca ctatctgccg gcgaacatgc tcgcgatcgc cgccggttca
    10981 ctgttcaact tcctgttgag ccacctcttc gtgtttcgcg gcgcgcgcgc aactcctacc
    11041 gctccgccgg cccccgcggc agagtgagcg tcaccacctc cagatcataa tccgtatacg
    11101 cgcgatacgt gcctcgcagc caaagtagcg cgaccccgtc tcccggccac ttcggcacga
    11161 tcgggcggat gttgtccact gccgagtccc gcgtcagcgc cgtccacgtc caccgcccgc
    11221 cgccgtcgct cttagcgccc cggtaaagct cccagtgcct ccttccatcg gtccggctcc
    11281 gaagaggcgc gcccgtctcg ggatctgcgt tagtggaaat gtaaacggtg ccgggatcct
    11341 cgggatcaag cgcgatgttg ccagtgtaat catcttcgcc tttgtacagg cgggttccgg
    11401 catgtgcgat ctccaggtcg ttccactcct ttccgtccca ccgcgcgtac cgataacgga
    11461 ggtcctgtcc ctcctgtccc accggagtct ctttgggatc cttctgcacc gagtacgcgg
    11521 caaagggccg tccgtcgcgg tcgagatgga tatcgctcac ccagccgacg ttctcggcgt
    11581 tccccgcaaa aatccgcgtg ccttcctccg gccttctcag accctcggcc aaactgcgga
    11641 tgacgctgcc gtcggaccgg tgcagtttcc cggcgcggta gaagatgtgg tacgtgctgt
    11701 tctcgaagtc tctcgggtgg ccgtcggtgt agaagatgtg gaccgtgtct gcgccgttcg
    11761 atgcgtactt cacgtaaggc cggtggcgga acgtcgccgg cacgtcgatg aatacgttgc
    11821 cggtcctcca cgtccgtccg tggtcgtccg agtacgcata ggagggcttg ttccgcccgt
    11881 ccaggccgcg gaagaaattg tagagccgcc cccgtccacc gttctcgttc cgcagataat
    11941 gcaggtttga gtaggtgatg cgtgacgact cgctcggggt gaacgtctgc tcggattgcc
    12001 acgatgcggc gtcgtccggc cgttccgaga tccgatagta gaaccggttc tccgggccgt
    12061 gcttggcgta agcggcgagg atatgtcgat ctggcaggac caggagcgcc ggcgagttgt
    12121 ggtcgtcgta ctgaaggttg gcgtgcagcg tggataacga cctggctccc ctgcgcaggt
    12181 cgaagacagc cacctcgacg ttgcctcgcc gagcgggatc gtgcgtaccc gccgctaccg
    12241 aaccggcgat cagtttgccg ttgtggacga tcgcgcgctc atcttcgaac caggaccacg
    12301 ccccgtcatc gttgatggtg aaaggtttct gccgatcttc cgcgtgcagg agggaggccg
    12361 cactgcctcc gagaaacgcg cgtctcgaga ttccgtctcc catgacgagc ctttatatca
    12421 cgcgtcatgg ctgcttccgg catggttact gtgcacggca tgatattctg ttctccggag
    12481 tgttcagccg ccatcaccgc aaggtcaggg tcctgttcgg cctgtcggat gtcctcctaa
    12541 cggcgctggc tttcgaggcc gcctaccaga cgcgcctgca cctccagctc gagcgggtgt
    12601 tcttcatcgt cgttccgacc aaggccctgc tgctgggtgt cactgtgctt gtatgggtcg
    12661 ccctcgggct ttggcttgag gtttacgaca aactcgacgc cggccaccca cgcgtgattg
    12721 ttcgcgacag cctccggcag tgcgtcctcg gcgcggttgc cgtgatcctg ttcgagttcc
    12781 tcctccggct cgacctcagc cggacgtttc tggctctttt tgcagtctac agttggtgct
    12841 tcctctgcct gttcagactg gcagccggaa agctggtggg aatcattcgg cgggaattcg
    12901 gagcaccgct gttcgtgctg gtggtgggcc tcggcgagcg ggcgcgccgc cttggcgact
    12961 cgctcgagcg atccgaaaag tacggcgtgc ggctcacagg cttcctggcg gacgagggtg
    13021 ggcccgcacc cgatcatgtg cagctttcca gaagctaccc tgtgtaccct atcgccgaac
    13081 tgcccttgct gttgcagagg cgtgtgatcg acgaagtcct ctttgctgtc gacagccgca
    13141 ggctggccga actggaggaa gtctttctgg agtgcgacga ggagggagtc cggacacgcg
    13201 tcgcggtaga cttctttccg cacgtcaaca gccgcgtcca tctggaggaa ctcggttcgg
    13261 cacccctatt gaccttcact gccgtcccgc acgacgagat ccggctgctc ctgaagcgcc
    13321 tggtcgacgt gacggtctcc ttcgcggggc tgctcgtgct cttcccgttg atgctgatcg
    13381 tcgccacctt gatccggctc acctccgccg gtccggcggt tttccgccag gtgcgttgcg
    13441 ggctcaacgg gaggcgcttc gtgctgtaca aattccgctc gatgtgcgag aacgccgagg
    13501 atctgaaatc aagcctcgag cacctcagcc acaggcggac ggcgttcaag atccccaacg
    13561 acccgcgcct gaccctcatc ggccgatggc tccggaagtt ctcgatcgac gaatggccgc
    13621 agttgtggaa cgtcctgcgc ggagacatgt cgctcgtggg accccggccg gccgttccgg
    13681 aagaagtcga gcagtacaag cgctggcagc gtagaaggct ccggatgcgc cccggtctca
    13741 cctgcctctg gaccctggcc ggccgcgacg cactagactt cgaatcgtgg atgaaaatgg
    13801 acatggagta cattgacaac tggtcgctcg cgctggactg gagaatcctg cttcagacga
    13861 taccccgggt cctgaccggg aaaggagctc actgagttta tgcacctcgt tccaacccaa
    13921 gaggaagtgg tcgcgctgct ggaacagacc ggcgctctgc ggcgaggcca tttcgaatac
    13981 ccgaacggtc ttcattccaa cgaatacctc caggtggctc tggcaatgcg ctactatcag
    14041 cacgccaagg tgttgagtgt gggactgagc cgccttctcc gggcgaatcc ggagatccgc
    14101 gcaatcattc cggaactttc aatcgtcgct ccggctaccg ggggtctgcc ggtggcatac
    14161 ggtgtttgtg aagcgctgcg cgcgcatcaa gtctactggg ctgaacagga ggacgacaag
    14221 cagcctctcc gcttccgcca gtacctcgag caacttcctg gggagaaagt gttgctggtg
    14281 gacgatatcc tgcgcaccgg caagaagctc acggcgctca aagcgctggt cgaggcgaat
    14341 ggcgccgagg tggtgggact ggcggtcatt gtccatcagc cgacgccgaa gacgcctgac
    14401 ttcagcccgc tgccgttcta ttacctcgcc aaactggacg gactctatta cagggacgcc
    14461 ggttcgtgcg acctttgcag gcagaacgca ccactggcga aggtctggat ctgaacgttc
    14521 agcgccatga gttacagtgc atcgtgccgg ggatccaaga taatggccag actcgcaatt
    14581 ctgatccttt tcgccgcctc cgccctagcc gctgatttca aggcgggcgt cgcacgtgtt
    14641 gacatcaccc catcgactcc gatctggctt tcgggttatg caagccggaa gcatccatcg
    14701 gagggggtgc tgcatcccct ttgggcaaag gctcttgcca tcgaggattc caagcggaac
    14761 cgcgtcgtca tcgtcaccac cgatctgatt gggctcccgc gcccgatttc cgaactggtc
    14821 gcggcaaggg tgcagaagga atacggtctc gaccgctcac aattgctgct gaactcctcc
    14881 cacacccaca caggtcctct ggtcaaggcg aacctggagt tgatgttcga cctaactccg
    14941 gatgacaagc gcacactcga ggcttacagc cagaaactca cggataatct cgtttccgtc
    15001 gccggcgccg ccctcggcaa actcgctccg gcgaacattg ccttcggcca gggacaggcc
    15061 ggcttcgcca taaaccgccg tgagcccgcc ggtggaggta tgcggattgg agtgaacacg
    15121 gaagggcctg ttgaccattc cgtccctgtg ctgaaagtca ccgcagcgga cggcagcctg
    15181 atggccgtcc tgttcggtta cgcgtgccac aacaccacgc tcacgggcga gttctacaaa
    15241 atcagcggcg attacgcggg ctttgctcag atcgaagtgg agaaggccaa tcccggcgcg
    15301 acagccatgt tcctgatgct gtgtggcgcc gaccagaatc ccaacccgcg cagttcgctg
    15361 gagcttgccc agcagcacgg caacaccctg gcggcttcgg tgggcgcggt gctctcgagc
    15421 aaagtgcaac gggtaaaagg tccgatccgc gccgcgttcc aaatcgttga gccggagttc
    15481 caaccgcaca cgcgggagac tttcgaacgg ctgctgaccg cgtcgaaccc cgtccaggtc
    15541 cgcaacgcaa agatgatgtt gcgcgcctat gatgatggcc gtccggtccg gcgcacgcct
    15601 tacccggtac aggccattcg cttcgggaag agcctcacgc ttgtcgccct tggcggggaa
    15661 gtcgtggtgg actatgccct gcggatcaaa cgcgagtacc cgaaggagaa catcgtcgtg
    15721 gccggctact cgaacgacgt gatgtgttac atcccctcac gacgcatcct gaaggaaggc
    15781 ggctatgagg cggtggacag catgatctac tacggacagc ccggaccgtt caccgaagaa
    15841 gtggaagaca cgattttcgg cacgattcac caggtaatga gccgggtcgg acgcaaggcg
    15901 ccgcgctgag gcgtggtgcg cggtgagcac ggctgcctcc tcatgtggtc gcatccaccc
    15961 gaaagcctcg aagtcgcctg cggcggcgtt catgtgtggc gtgccgcgct cgacgtatcc
    16021 ccagaactgc ttgtaaggtt ctctgatcat cttgccgaag acgagcgttc ccgcgccgca
    16081 cgctttgttt ttccgaggga ccgggaccgg ttcatcgttg cacgcagcgt ccttcgagat
    16141 attctcgggc gctatctggt cctccatccc gcggatatcc gcttttccta tggagaatcc
    16201 ggcaagcccg agctggcgcc tgacctttcc ggaagccgtc tgcgatttaa cctggcccat
    16261 tcacgcgacc tcgccttata cgcgttcacc ctcggacggg aagtcgggat tgacgtggag
    16321 ttcgtgcgct cggagcttgc gaaagaggag attgccgagc gcttcttctc ggagcgcgag
    16381 gcggcggagc tgcgggcgct gccgccctcc gagcagccgg cggcgttctt ccgctgctgg
    16441 accagaaaag aagcctacat taaggctcgt ggacgaggct tccgtattcc gctcgccagc
    16501 ttcgatgtct cactaggaca gccggctagg ctgctggcgg ttcgggacga ccccgccgaa
    16561 gccgcacgct ggatcctttg tgacatccca gtcggccggg attgtcaggc cgccctggcc
    16621 atcgaaggag agaaccctga attcttcctc ttggactggc tctcggctac gtaaaccgcc
    16681 attcctgccc gacgtgctgc gtcaccaccc gttccgggct cctttcggcg gcctccagaa
    16741 agcgctcgat cggttccgtc agcggctcgc ggctgagctg gaaagtctgg tgatgcacgg
    16801 gaatgaagaa ctcggccccc gcgtcattcc ccatccgcca cgcctcctcc ggcgtgcagt
    16861 ggtaacgaat ccagggattg tacgctccga tcggcataac ggcgaggtcg atcggccggg
    16921 atgacttcaa gaccctgaac ttgtccgtaa tcgcgctgtc gccggcgaaa aggactctgt
    16981 agcgcccgga ctcgatgaga tacccgttgt aaccgcggta tgtatccgag cgcattcgag
    17041 caccccagtg attgacctcg aaggcacgta tctcagccgg ccccacccgc gttcgctcgt
    17101 cccagcgcag ctctgtgacc tttctgtaac gatccaccct gagtagatcg ctggtttccc
    17161 aagcggtaat cactggtgtc cgcttgtcct ccagatgacg cagcgacgga atgtcgaagt
    17221 gatccatgtg cgcgtgggac agcagcacaa gatcaatctt cggaaggttg tcgatgccga
    17281 gcgccggcgc gactaggcgt ttgagcccga gcgtcaacgg gccaaagttc aatccggcgc
    17341 gatcgctgaa aaccgggtcc gtcaggatgg tgaatccatc gatcttgagc agtacggtgc
    17401 tgtggccgag ccatgcagca tacagccccc gatccggcca ctcggaaggt cgaggacgga
    17461 ccggcggccg ggcgatttcg cgccccatct cgcggacgta ctgccgccag aagctcggag
    17521 cctgccggta gagcatcgtg ccggccccgg cgagtcccgc gacactcatc ccgccgatcc
    17581 acgcccggcg gctccacctc gtcgagccgc tctcaccagc cataccgttc ctccgtccac
    17641 gggtcaccgt gcatgtggta tccattccgc tcccagaaac cgggacgatc cttctccatc
    17701 aactcaatcc ccgcgagcca cttggcgctc ttccaagcgt acaattgcgg cacgaccagg
    17761 cgcgccggcc cgccgtgctc caggctcagc ggttcgccgt cgtgcgagat agcgacgaga
    17821 gcgtcctcag caaggaaatc cgacaaaggc agatttgtcg tccagccgcc gtcatagccg
    17881 tgcgcgagca cgtatcgggc gtccggcgtc ggtcctcccg ctcgcgccag aatctcccgc
    17941 gtcgagacac cttcccacag gttgccaagc cgggaccagc gggtcacgca atggaaatcg
    18001 gcaaacacct tgaccctcgg gagctccaga acctgctccc aagtccactc tatcggcttc
    18061 tgaacccccc ctccaatacg taatcgccag gtcttcagat cgatccgcgg gggacctgaa
    18121 gcatccagca ccggccattt ctgcgtgcgt gtctggcctg gcggcagccg gcgctcccgg
    18181 cgggtgtccg ggctgacaat ggcgtccccg gcagcctgag gttcctgcaa ctggtttgca
    18241 tcagcgtggg acataatgct tagatacgct tcagtgaata gatgaaatct ggaccagact
    18301 ggttgcaggc tgggccaccg gcttgaagga aaggacgtcc atcgggcaga ccgtcacaca
    18361 gatgccacag ccgatacagg tgctgttccg gttgttcaac tcctcttgtt tcagtgcgaa
    18421 atccatgaca tcgatgccca cctggcagtt ccggctacac tcgccgcacg cgatacactt
    18481 ctcgtttgcg tggattgcga agcggctcca ccgcagccgg gtgaagaccg agctgaagag
    18541 ttccatcatc ttcgccagcg ggcaccaata ccggcaccag atcttgccgc cgaaaaacgg
    18601 atacagcgaa acgggcagaa tcccgacgag ccagacgtcg gccgtcagcc ggtaagccaa
    18661 aactccggcc tcggccggcg cccgcagcac gcccacgacg tcgcgcagca gcaccatcag
    18721 agtcaccgcg gccgctgcgc cgaacaccac acggttcatg aactcccacc gaatgctggt
    18781 cttacccttc ggcgcaaggt gccgccaccg gtccccaagc gtctccgcca gcccgccaca
    18841 gccgcaaatc caggaacagt atcgctttcc gtgaaacacc gcgaggatgg gaatcagtac
    18901 gaagctgagc acaacgcccc aaaccaccca gatctggtgc ggagacccaa agaaggtgta
    18961 gaagaacagc ggccacgcgt agacgattcc gtacgaccgc caggcctgct gcgcaaacgt
    19021 cggatcctcc gccaaccggc tgccgaccca ctggtattcc accgcccagc gaaacagaaa
    19081 ctccggaatc agaaagaaga acacccactg gaagctgagt aggctgacgt agcgccaaat
    19141 ctggaagcgg tccttgcggt ccagtcccca acgcttcatc gcctgaagcc caaagaccgt
    19201 gatcaccgtc gtgtagagga ccgtgtacca aaacgaccac gggcggccga ggaacgacag
    19261 cgccttatat ccccatcccc ggaacggcca gaactccagg ttttctccga gctttgctcc
    19321 gtagatggtg tacgccatga ggaaggtcag tccgagccag gcaaaacggt tgcccttcac
    19381 gccggtggct acaaggccgg ccagacccgc cagcgcgaaa agtccacccg ccatcgtcag
    19441 ccctgcgaaa actggcattc cggtcttgcc gccgaatacc cagagcccga ccatcgtcgc
    19501 gagcacgaga accgcggcgg agaagacact tccgttccag tcgttctcca gccggatgcc
    19561 gagtgaatgg aggaatttcg ccggcagttc cgcgccgatc aggacgaagg catggtcgta
    19621 cggaagttcg cgtgtgctcg tggcagggcc tttgcgaatc tcgagcacgc acgttctctc
    19681 atcaaactgt ttgacgttgg aattcaggat cactcgcagc caccccgccg ccaccgcctc
    19741 gtccagtctt cgccgattgt ctttgaacag ccgcgaaaac tcggttcccc ggtatgacag
    19801 cgtgaccctg ttccgttcag cgagcgttag cgccgcctcg accgcgctgt tgccccctcc
    19861 caccacgaga atgtcctcgt cctggtattg cttagggccg tagagccggt gatagacccg
    19921 ttcgcggtcc tctccgggtg cattcagttt gcgggggttg ccgcgttggc ccgtcgccag
    19981 gaccacccgt cgggcacggt acgttcgctt gggcgtctgg acgtcgaagt ctccgtcctt
    20041 ccgcttttgg atatgggaga caccctcttc cgtcctgacg tccagcttgt tctcgcgtac
    20101 aatctcgcgc caccggtcaa tcagttcttc ttttcgcgcg ccgtccagcc acagttttcc
    20161 cttcggaggg gaggaatcag gctcggcata gacccacttg ccctcgggaa actcctctat
    20221 cgtgttggaa atcttgctct tttcgagcac gaggacgcgc agaccgtggt cctgagcttc
    20281 cagcgcgcag tttaaccctg ccgcgcccga acccgcgaca atcaggtcga agacgtcagc
    20341 atcgcgcccg tgggcacccg gcttggagga gatgtgaagg gccagctcgt ggccctgctc
    20401 cattgccagt ttcacaacgg gcgcgccggc gagatcgccg atgatataga gccctttgac
    20461 gttgctctca aagtgcttgt ccacaacagg ccgtcttgtc gacccgttcg gactctcgag
    20521 gatcgcgtgc caaagcgact ggatgcggtt caccgtgctt gacctgctta cgggagtccg
    20581 ggaaatgcag ccagccggtt tcctccccta ctaccatcaa cataccgcag cgctcgcgag
    20641 acattcccgg ccatatccgg cgccgggcga aacccacgta aagatattcg gctgtacgct
    20701 tcgctggttt gactctcctg gtaggccttg ctagtctggc ggtttcatgc ctgaggcccc
    20761 cgtcctgaag cggacttcgc ttcattccct gcatcgccgg ctcggcgcca agatggtcga
    20821 cttcggcggc tgggaaatgc ccgtgcagta ttcgggcatc ctcgacgaac accacgccgt
    20881 acgccgttcg gtcggggtct tcgacgtcag ccacatgggt gagattgaga tccgcggccc
    20941 ggaggctctg cggctggccg agtatgtcac cacaaatgct gtctctcgcc tcaagacagg
    21001 ccaggcgcaa tactcgggac tcctctacga gcacggtggt ttcgtggatg acgtgctggt
    21061 ccacaaagtc gaagacgacc actacttctt ttgcgtgaac gcctcgaatc aggagaagga
    21121 cttcgagcac atccgggatc gaaaccggtt caacgccgcg gtcgacttcg cctccgaccg
    21181 ctatgcccag attgcggtcc aggggccgaa gggcctggca acagtgcaga agctgacgtc
    21241 gacggatttg tctgccatcc gctactacta cttcaccgac ggcgaagtgg ccggatttcc
    21301 ggcgcgtatc gcccgcaccg gctacaccgg ggaggacggc gtcgaggtct atgtggcgcc
    21361 ggagaatgcc gaagccctgt gggatcggat gttcgacgcg ggtcaggagt ttggcgtcaa
    21421 gccctgcggc ctcggcgccc gcaacacgtt gcggctggaa gcgaaaatgg ccctgtacgg
    21481 tcacgagatc gacgcatcga ttaccccgct ggaagccgac ctcgcctgga tagtgaagat
    21541 ggaaaaaggt gatttcatcg gccgcgccgc actcgagcgc cagcaacgcg atggcgtccg
    21601 ccgcaagctg atcggcttcg aaatgcgcgg acgcggtatc gcacgcgacg gctacgaggt
    21661 tctcgtggac gaccggccct gcggctgggt taccagtggc gggccctccc ccaccttgaa
    21721 caagaacatc ggtctgtgtt atctccccgt ggaactggca aacgtgggtc tcccgatcca
    21781 ggtcgtagtt cgcaatcagc ccgtggacgc ggaaacggtc cccacacctt tctataagcg
    21841 agcaaaatga cgatgtatcc tgacaacctc cgttacacca aagaacacga gtgggtcgaa
    21901 gtcgaaggcg atatcgggaa ggtgggcatc acgcaccacg cacaggacca gctcggcgat
    21961 atcgtttacg tggatctccc gaaaccgggc gcccgcgtcg agaggggtag gtcgttcggt
    22021 tcggtcgagt cggtcaaggc ggtttccgac gtcttctcgc cggtatcggg tgaagtcgtc
    22081 gaaatcaacg aaaccctcgg cacagccccc gagaaactca accaggaccc gcatggcgat
    22141 gcgtggctgg tcaggatccg gatgagcgcg ccggacgaaa tcaaacaact catgaccgcc
    22201 gcgcaatatc agaactacat aggagcagaa atctagcttg cgttaccttc cgaagtcgga
    22261 ttcggagcgc cgcgaaatgt tggaggcgtg cggcgtttct tctctcgacg attttttctc
    22321 tcacctgcct cccgatgttc ggttgaaccg ccagctcgca ctccccgctg gccagtcgga
    22381 gtatgagatt tcagcctatt tccgcgccag ggcggaccag aacgcaaccg gctacgcgtc
    22441 gttcctgggc gcgggcgtct acaaccacta tcgcccggtg ctggtggaca cggtcgtatc
    22501 ccgcggggag ttcctgacct cgtatacgcc gtatcaagcc gagatttccc agggcacgct
    22561 gacgacgatc ttcgaattcc agacgatgat ctgccaactt accggcatgg aggtcgccaa
    22621 cgcgtctatg tactgcggct cgaccgcagt gccggaagcg gggatgatgg cagcccgcat
    22681 caccggccgc aaccggcttc tcgtggcaag gactctgcat ccggagtacc gcgaggtgct
    22741 gaaaacgtac gcccggtatc agggcatggc agttgaggag ttcggttaca ccgcgggcca
    22801 cggaaccgtc gatctcgacg atctcgaccg caggcttgat gacaagaccg cggccgtgat
    22861 cctgcagtcg ccaaatttct tcggcatcgt cgagagcgtg gacgccgccg ccgacctcgc
    22921 ccataagagg ggagcgatgc ttatcgacgt attcaccgaa gcggtgtcgc tcgggctgct
    22981 cgaaccgccg cgccaggccg atatcatcgc cggcgaactg cagtccttcg ccatttcccc
    23041 gagctacggt ggtccttacg cgggtgtcat cgccacacgg gagaagtaca tccgccaaat
    23101 gcctggacgg ctcgccgggg aaacgaagga ctcgcacggc aaccgcgcct actgtctcac
    23161 tctcgccacc cgtgagcaac acatccggcg cgagaaagcc acgtcaaaca tctgcaccaa
    23221 ccaggcgctg attgcgctga tggcgaccgt cttcatgacc gtgtacggca agcagggggt
    23281 gcgggagttg gcggaacaga atctggcaaa ggcgcactac ctcgcaagcc ggctgccacg
    23341 gaaattcgcc ggcccgttct tcaatgagtt tgttgccgcg ttccccggaa agtcaccgga
    23401 aacgatcaac ggaatgatgc tcgagaaaaa gatcataggc ggactcccac ttgggcgcta
    23461 ttacccggaa ctagaggatt gtatgctcct gtgcgccacc gagatgagca cccgggagca
    23521 gatggacgtc ttcgcggagg tgctggcgca atgatttcga aagttcgctc gcacgtcacc
    23581 cagaatgagc cgctgctgtt tgaacttagc tctcccggga agaaggcgta ccagctcccg
    23641 gagctggacg tgccgccggt ggacgcctcc aaggcgctcg gcgcggaaca cgtccgccgc
    23701 gaggtcgaag gattccccga ggtcagcgag gtcgaggcga ttcgccattt cacccgcctg
    23761 tcgacgtgga actacgccat cgacctcggg ctatacccgc tcggctcctg caccatgaag
    23821 tacaatcccc ggctcaacga ggctgtcgcc aggatagagg gacttgcctg ggcgcacccg
    23881 taccagccgg agtccttgtc gcaaggcgcc atggaaatca tgagtgcgct cgagagcgcc
    23941 ctcatcgaaa tcaccggcat ggacgcggtc accctccagc ccgcggcggg agcgcacgga
    24001 gagctgaccg gtatcctgct ggttcgcgcc tatctggaat cgctgggcaa ccctcgcaag
    24061 aagatcctga tccccgactc cgcccacggc accaaccctg ccaccgcggc gatcgcgggc
    24121 tatgccgtcg agaacatcaa gtcaaacgac cgcggcatga tcgacctcga actccttgcc
    24181 cgggtggttg acgaagatgt ggccgcgctg atgatcacca accccaatac gctcggggtc
    24241 ttcgaagagc agatcgcgcg catcgcagaa atcgtccact ctaagggcgc gcaggtctac
    24301 atggacggcg ccaacatgaa cgctctcgtc ggcatcaccc gtcccggtga ctttggcatg
    24361 gacgtgatgc acctgaactt gcacaagact ttctccactc cccacggcgg agggggtcct
    24421 ggcagcggac cggttgcggt caagaaacac ctcgaaccgt tcctaccgtt gccacgcctg
    24481 acggccggtc acgccggcct gcgattcgac tacgaccggc cgcgggcgat cggccgtgtc
    24541 aaggctttct acgggaattt cggcgtgatc gtccgggcgc tggcatacat catggcgcac
    24601 ggcggcgagg gcctgcgcaa cgcaacgatg gatgcggtgc tgaacgctaa ctacatccgg
    24661 aagaaactgg aaggctatta cgagctcccc caccgcgctc ccagcatgca cgagtgcgtg
    24721 ttcagcgacg cccgccaggc caaacagggc gtcaagacca tggatatcgc caagcgcctc
    24781 atcgactacg gtttccaccc gtacaccgtc agcttcccgc tgatcgtcca cggcgccctg
    24841 atgatcgagc cgacagagac cgaaagcaag cgtgagttgg atctgttcgt ggcggcgctg
    24901 atcgccatcg cgaaggaggt cgaggaggat ccggaaatcg tcctgaaggc gccttacggc
    24961 actcgcaccg gccgcgtcga cgaagtcacc gcggcccgca agcccgtgct ccgctggaaa
    25021 ccggcggact gagcagcctc cgcaggcacc cgatgacccg cccgcttctg ttcgtcctcg
    25081 cagtggcgct gctcatccgt ctgccgttcc tgaaccaggc catccagggc gatgacgtct
    25141 actatctcgc gggcgcccag catgcgcaga tcgacccgct ccaccccaac cacgcccgtt
    25201 acgcctacct cggcgatgtg gtcgatatgc gcggccatcc gcacccgccg ctgaatgcgt
    25261 ggttcctggc cgggctgctc gccatgttcg gcgatatcgg cgaagttcct ttccacgctg
    25321 cgtacatcct gttcaccatc gtcgcggcgc tggcgatgtg gtccttggcg cggcgttttt
    25381 ctccgcgtcc gctggccgcg acgctcctgt ttctggcgac gccggcgttc gtggtcaacg
    25441 ggaactctct cgaatccgac atcccgttcc tggcgttttg gatggcctcg atcgccctct
    25501 tcgccggcgc tttggagacg cgttcccggc ttcggctcct cgcctcggtg ggcgcgatgg
    25561 ccctcgcggc gctggcggcc taccagtcgg tcgtgatggt cccgatcctc gcgctccact
    25621 tatggtggaa cgcgaggaac tggcggccgg catgggcggc cgttctggct tcgcccgtga
    25681 cccttgtgat ctggcagatc tgggagcggg cgtcgacggg agagattccg gtcgccgtac
    25741 tcggcgacta tttccagact tacggatttc aggcgctgct cccaaagctt cggagcacag
    25801 tagctctcac tgcgcattcg gcatggattg tcttcccagt gctcgccctg gtcgcattcc
    25861 gccgcgcggg ccgcaccgcg tggattgccg cgctcgcggg cgcagcaggc gcggcgtttc
    25921 tcgatccgca tccgctgttc tgggcttcgt tcgggacagg agtactcgtg ctggcatggt
    25981 gcgccggtcg gctgcgttcc gctagcatgg aggaacgctt cctcgccgcc tgggttgtgc
    26041 tcttctttgc cggcgccctg gttctgtttt tcgccggatc ggcccgctac ctattgccga
    26101 tggccgcccc gcttgccctg cttgcttcgc gagccctcgc ccaccgccca agctggctta
    26161 ccgcggcgtt cgtcgcccaa ctggcgctcg gacttgcact gagtgcggtg aattttcagc
    26221 actgggacgg ataccgggcg tttgcccgcg agttgcagca cgaaacggag acgcggcggg
    26281 tatggattaa cggcgaatgg ggccttcgtt tttacttcga ggctgacggc ggtctcccgc
    26341 tgatgcgcgg gcaggccgtc cagccgggcg agatggtagt ttcgagcgag cttgcgttcc
    26401 ccatccggtt cacgaccggc ggcggcgtgc tcgtccccgt gaaagagcat gagatccggc
    26461 cgtcgctccc gcttcggctg ttcggactcg gcgcccgctc cgcttactcc agcgcgtccc
    26521 tcggattccg cccgttcgat ttctcgaccg gtcccgtgga tcgcgtccgg gcggatctgg
    26581 tcgtcgagcg gaaaccgtcc ctttcgtacc tcccgatgaa cgcgtccgag gcgccgcagc
    26641 agatcgtgag tggcatctat gagttggaag gagggcaatg gcgatggatg ggagatcgcg
    26701 cggtcctgtt gctccgccgc cctgaggccg cccttccgct gcacgtcgcg cttcacatcc
    26761 ccagtcaatc gccggcccgc cgcatcacgg ttactgtcga tggagtgcag gtcgccgaca
    26821 agacctttgc cgcccccgga acatacaccc tcacttcgga gcccttcgtg acggcgtcgg
    26881 atagcccgac agtcaccatt accgtgaacc ggacattctc agtcgcgggt gatcaccgca
    26941 agcttggcgt cattctgagc gctgtcgggt ttgagcgctg agtagttcgc gggcagccaa
    27001 aagcacctcg tcgacctcg
//
LOCUS       DATIFQ010000277        23756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_856477, whole genome shotgun sequence.
ACCESSION   DATIFQ010000277 DATIFQ010000000
VERSION     DATIFQ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 23756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23756
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856477"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..376)
                     /locus_tag="VLE22_12955"
     CDS             complement(173..376)
                     /locus_tag="VLE22_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15362.1"
                     /translation="MNFVCLLYYLLVIRKARQLQNLVVTTTPKEDYEVNLWVGRSLRG
                     SGEVDVELTVDGKNVNTVRINIK"
     gene            complement(452..1246)
                     /locus_tag="VLE22_12960"
     CDS             complement(452..1246)
                     /locus_tag="VLE22_12960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSB15363.1"
                     /translation="MKALVMLAIALTPTIGYSQAPVITGAVNAASFAQDQPLSPGSLV
                     SLFGTGLAASTATASTVPLPDSIAGVSVTFSGITAPLHFVSPSQINLQVPWRVEPGVT
                     DIVVTVSGSTSAAFRAQVEPVSPGIFTISSRSRQAVAINADGSVAGEYESIPGVVARP
                     ARAGEIIMILGTGFGPVTPANADGAASRDQLRTTIATPAVFINGIPALVTFCGLSPEF
                     VGVNQINVVVPHNPTGAGGEWPVQIEIDDMRTSDKVTITVMGAWDY"
     gene            2007..2498
                     /locus_tag="VLE22_12965"
     CDS             2007..2498
                     /locus_tag="VLE22_12965"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HSB15364.1"
                     /translation="MQKNQLTKDIAIALITGVTLLWCAAPMQADETSKAAKAEELLQL
                     TQGDQMMKSFLAPMKGVLTADMNKTDMPAEKRAKASEMQAKIMTMIADRLSKARPALV
                     KAYTDTFTEGEIDGILAFYKSPAGQALLQRMPQVMERSMPVIMQLMGDLQPEIKRMTE
                     ELK"
     gene            complement(3996..4084)
                     /locus_tag="VLE22_12970"
     tRNA            complement(3996..4084)
                     /locus_tag="VLE22_12970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4048..4050),aa:Leu,seq:caa)
     gene            complement(4131..4895)
                     /locus_tag="VLE22_12975"
     CDS             complement(4131..4895)
                     /locus_tag="VLE22_12975"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HSB15365.1"
                     /translation="MTKNRVKQALKEGQIQLGTSFGQFRCPEISRLLAAAGFHWAFID
                     TEHGGFGVETVQDVCRVANLAGLCPLARVAELQYSLITRALDCGAQGVIFPRVEEPAL
                     LERAVSWTKFPPAGIRGYGLTAMQTDYESASFPEVIGHVNGNTMVVLQIETRRAVEAC
                     GELLSVPGLDAVMVGPADLSISLGVPGEFQHPTMVEAMEKVRDACIERGVAPGTQTRT
                     LKLAQFWKERGMRFLGCSSETGMLFERARELASQLA"
     gene            complement(4905..6482)
                     /gene="bshC"
                     /locus_tag="VLE22_12980"
     CDS             complement(4905..6482)
                     /gene="bshC"
                     /locus_tag="VLE22_12980"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HSB15366.1"
                     /translation="MESRCLRHTELPHTSRLFEDYVYHFEKVAGFYELPPRQPASFQE
                     AVSRLDFPDERRTALVSALREQNGDHPTLDRLAHPGTVVVVAGQQVGLYSGPAYTIYK
                     ALTAVKLAARLTEQGMPAVPVFWLATEDHDFAEVDHCWIFDAAHRPVRLEARSPARTD
                     GPVGEAPFSGELTGELRRTLSGFAFGDDVAALVEQTYTEGRSFGAAFHELLRRLLEKF
                     GLVYFDPMQPALRELAAPVLRRSLEAAGDLVPAVLERNSALAAAGYHAQVHVESQSSL
                     LFLLEGGRRLPLRRTEDSYIAGTLRISADELAGRAESLSPNALLRPVVQDYLLPVAAT
                     VVGPAELAYLAQSQVLYRTLLGRMPVVVPRAGFTLLDSRSDRLISRYGLAFGDFYHGE
                     GALRERIASRLVPPGLSRCLWEASAATEKHIERLRAELVRFDPTLAAALDTSRRKIVH
                     QFTKLEHKVARESLRRDERAAEEAAYVYGLVYPERHLQERLYSILPFLAKHGLELLDA
                     IYDNVQLDCPDHQVLVV"
     gene            complement(6543..7439)
                     /locus_tag="VLE22_12985"
     CDS             complement(6543..7439)
                     /locus_tag="VLE22_12985"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HSB15367.1"
                     /translation="MRQNEARRHWRKPVDCELSLSWQDKVGNTRFIRARGLDVSEKGA
                     RIETEERIDPNSYVFINMDQYGLAGSASVRHCTRRDGKFVIGLEMNDVKRGDDIPDSE
                     DLADYYEFLQISPNAETETIQRVYRMLVVRLHPDNPETGDCEKFLQLNRAYRTLIDPQ
                     RRAEYDSRLNRRRSEPLKVFELKEFIGGVEGEANRRLGVLCLLYMRRRSNPEDPGLSL
                     LEFEQMMSFPREHLMFTFWFLKEKGFIRPENNGDCVITALGADFVESSIPAHRSLYNL
                     LHAPEGEPAPYARDLEVEHSAA"
     gene            7847..11293
                     /locus_tag="VLE22_12990"
     CDS             7847..11293
                     /locus_tag="VLE22_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSB15368.1"
                     /translation="MANWVSRIRKTVRTWREGRHSLAVVVSMLCVGLFLLAICRVFWV
                     RAARVERCVELANRQLELSREFLSLVRQPKGRPGDWSGGDPLLITEFDTALALLEDHF
                     SQDRGVLPLPTRLSRGLAEVRRLWPEIKEPLRQAAARRGTEDARHLEPKVIAMVEAWD
                     GLGDATRQRRLALQQWLICVMAAITAAALMLLYSGAWVIAAGGTRARRFVEAIVRHAA
                     NGPFGDPSRAAARTDLAKLPRMFNGLSEAMGAVLEYVEENSRAVEDIADRLPSGVLVF
                     AQDLSVRYANRAFVDSLRLTEPDIRGMSLGTVVVCEPLTRWARHVLETRTSVSNVLVE
                     AGTNGDKRILRARLAAFQPRNTAEVQGLIVTEDYTEFDFLRSAREESEQRYRRLLESS
                     AEAVVVVREDGTILEMNKLAETAFGYSCREAAGLPLVAIAQESGRVVPEHTLSSLLRS
                     GMWQLHGTTLEYEFRRRDGTVFPGEVRLAACQAETGRLYFVSIRDIADVKRSEILAKD
                     RLEVIEMIARNQPLEAVLAGLAQMLERQVPGARCAVMLLRDNRLQPAAAPNIPESFVR
                     ALSGMRIGPDAASCGSVAFQGELTVVSDIASEPGWEEYREAALSHGLRAAWSAPIHSS
                     EGTIVGTLALYHGEPRQPNRGQLDLMQMACRLAAVSIEQRQLNGRLNYQAEHDALTGL
                     PNRFHFENWMQKQLLLARRHRRRVGVLVVDLDRFKLINDTLGHAVGDGLLRQIAGRLR
                     EHVRETDMVARWGGDEFVVGLLELRESRDASLVAEKLLDAMRTAFEVEGNELFATFTI
                     GISIYPRDGADLDALVQNADRAMYRSKNQGGNAFRCYSPEMGRTVAKHLELETSLRRA
                     LERHELLLHYQPQFDLRSGELVGVEALLRWSSHRFGLVPPSDFIPIAEETGLIVPIGA
                     WALREACRQIRAWQDAGHTPVKVAVNVSVLQFAKADFVEIVARALEETSVLAELLELE
                     MTESLIIRSLEESTVRMARLRSLGVGLAVDDFGTGYSSLAYLHRLPIDTLKIDQSFVR
                     DLSVSSSTRALVQSIVSLAKNLGMRSTAEGVETFSQMGALRRAGCSHAQGFFFGKPMA
                     PEAVALLMDRQKAARTNGPPFQIITPMPREDTTSKQARLHVLPPPVRRPAYRGGENLT
                     VV"
     gene            11382..11711
                     /locus_tag="VLE22_12995"
     CDS             11382..11711
                     /locus_tag="VLE22_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="frataxin family protein"
                     /protein_id="HSB15369.1"
                     /translation="MRDDAEFKHHADNAIHSVYLALTAASDDYAYDVSYESGVLTVEI
                     KRPRGRLVVDAHPAARQIRIFSPSRSYKLDWDVVENEFLLSGTGENLKEAMEEALSRH
                     LKEDVAL"
     gene            11718..12815
                     /gene="hemW"
                     /locus_tag="VLE22_13000"
     CDS             11718..12815
                     /gene="hemW"
                     /locus_tag="VLE22_13000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HSB15370.1"
                     /translation="MPAVYISYPFCRQKCTYCNFASGVFPRSLEERYRKALLAEIGSH
                     IWRWRPETLYLGGGTPGDIEAEGLEAILAAVPGHPWLEVTIEAAPGSIARDLVDVWCR
                     LGVNRVSLGVQSFIGGEIARTGRKHTAHTVRDEVALLRNGGITNINIDLIAGLAGQTV
                     KNWSESLDWIERLAPPHVSVYMFELDEDSRLGREILQGGARYGARDVPSDDRTAEMYE
                     TAVERLLAMGLERYEISNFALPGHESRHNLKYWRLEPYTGFGADAHSFDGDMRRQNVE
                     APQDYTERVERGESPCIGAVRAQAEEERFFVGLRLTAGIRPRPKEWQRFERPIRRFLG
                     EGLLETDGETLRLTSRGILVSNEVFQEFVDI"
     gene            12812..13873
                     /locus_tag="VLE22_13005"
     CDS             12812..13873
                     /locus_tag="VLE22_13005"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HSB15371.1"
                     /translation="MNPSEWTDLRSDTVTRPTAAMRRAMAEAEVGDDVYGEDPTVNRL
                     ERRAAEIFGKEAGLFVPTGTMGNTIAVKVHTNHGQEVICEARSHVLSYELAMMAWFSG
                     CVARAVRAEDGVLRWSRIRPEIRLLGPHWAPTGLIEIENTHNMAGGTVMPVEAIEEIC
                     DGAHGLGLKVHMDGARVFNAAAHLGRPVSEITAKVDTVMFCLSKGLGAPVGSMLVGTV
                     ADMARGRLYRKRLGGGMRQVGVLAAAGLIALEEMPNRLGEDHENAQFLAEALARIPGI
                     RIPHEVQTNIVIFDVSGTGLASSEFSARLKKRGVLINGINPTQMRLLTHFDANRTACE
                     RALEAIGQVAAGAAVAKEA"
     gene            13873..14703
                     /locus_tag="VLE22_13010"
     CDS             13873..14703
                     /locus_tag="VLE22_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB15372.1"
                     /translation="MPFTLQGKAALVTGAGRGIGRAIAEQLAAAGAAVMVNDLDDPAA
                     AETEAIIRNAGGRAERLAGDVTHPDFPERLVNGALRAFSSIDIIVNNAGYTWDNVIQK
                     TTDEQFQAMLDVHITAPFRILRAASGWIRETAKREMSEGRRVMRKVVNITSISGVDGN
                     AGQAGYSSGKAGVIGLTKTLAKEWGRYNVTVNCVGFGIIETRLTQPLGQEGAAIDIRG
                     RQIPVGVQPATLEALRAMSPLGRLGTVEEAAGSVLFFCLPLSDYVTGEVLLCSGGVHF
                     "
     gene            15533..15754
                     /locus_tag="VLE22_13015"
     CDS             15533..15754
                     /locus_tag="VLE22_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15373.1"
                     /translation="MAKLTEYTYRPSKITASTAPIAILILSLLLSQDFRFGFLAHCAQ
                     AAFAEKSYKEWGSAPKRAGAVRKEIVLPV"
     gene            complement(15932..17008)
                     /locus_tag="VLE22_13020"
     CDS             complement(15932..17008)
                     /locus_tag="VLE22_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15374.1"
                     /translation="MADTLNSSLELPSAGGFTISEIRQQPDTWPVTFERVREAPLGRV
                     SPPVITGAGTSAYAAIAIESAWPGSRAIASTELLFDFEEALSSLGTIVSLARSGDSPE
                     SVAVVEKVQKRLPSVRHIALTCNPNGKLANWPGVEAVLLDPLSNDRSLVMTSSFSNLV
                     LAGIALSRPAELGQALPVLCAGARTVIGKYEATARALAAEPPARVVALGSAPLFGAAR
                     EATLKVLEITSARIPAIPETYLGLRHGPMSFVNADTLVLCLISSDPKRRRYEMDLVNE
                     LRAKKIGRLVGLAPADADTSAFDEVVTTEASSLPDYLRTPAEIVFCQLLAYHLSLRFG
                     LDPDNPSPGGVINRVVQGVRIYED"
     gene            complement(17070..18689)
                     /locus_tag="VLE22_13025"
     CDS             complement(17070..18689)
                     /locus_tag="VLE22_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HSB15375.1"
                     /translation="MGLKQKVLSWIEHRTGLETAIRNFFYEDIPASSGWPQVFGSIAL
                     FLFLVQAFTGILLAFNYAPTPGDAYNSLKYIVTELTGGALIRGLHHWGASMMIIVVVL
                     HMIQVFLFGAYKKPREATWIVGVTLLLLTLGYGLTGYLLPWDNRAYWGTVVTTQLAGK
                     APVAGPYLNRLLGGEGSIGVVTFARFYALHVLLLPPLTMFLIALHVYLVRKHGVAPAP
                     GDAAPGKKFFPEQVLKDTIAIFAAFIVLFVLAVAVRVPLDRLADPTDTTYIPRPDWYF
                     LFLFQSLKFFTGPLEVVGSVVLPGFAVLVLFLVPFVDRGKVMKLTQRTYAAAVVVLAA
                     IGWAGLTAAAVATRPKPQETQIAAGVGEWRQLSPEELAGIGYFRQENCTSCHSVGNGG
                     ANVGPDLARISIRKNAGWMIQHFKNPSKMVPGSSMPPIQLSDAQLNALSAFLLKLTPQ
                     NAHALESAPAFAVQGALVFQAAGCSACHQVNDVGMKIGPPLNGLSKRRTREWVVEHFL
                     DPQKLSPGTVMPPYRFTPRQMQAITSYLMSL"
     gene            complement(18692..19189)
                     /locus_tag="VLE22_13030"
     CDS             complement(18692..19189)
                     /locus_tag="VLE22_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HSB15376.1"
                     /translation="MNETRGSDRRYFCVTCIYSLWGLIGLALGLPAAVYLLVPPKPRK
                     ESEWVEAGDVSQLEPGVPNELVFRRNRVDGWKITSEKTSAWVVKVSDADVVAFSPICT
                     HLGCAYHWDERKNNFLCPCHSSTFGLDGRVLSGPARRPLERFRVRIENGRLLLGAAER
                     STEPA"
     gene            complement(19386..21884)
                     /locus_tag="VLE22_13035"
     CDS             complement(19386..21884)
                     /locus_tag="VLE22_13035"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15377.1"
                     /translation="MTIVATPHSSPEADELRAAFFRDGDAFPVLAGRSAMVEAAVVRS
                     FDEFIAPALPGGAALLAVGGFGRRELFPHSDVDLLLLVPVELSEAGQRDAVSRFLQNL
                     WDSGLRLGHSVRTPTECCEVHDGNVELSISLLDRRFLAGDPALFERMEEKLPRFFSAR
                     RDELSRQLCRLTRVRQARYSDTIYHLEPNVKEAPGGLRDYQLARWLSQLRNAQSYRRS
                     EPDPFPELANARTFLYSVRCFLHYRTGRDSNLLGFDAQEELADQPFSPASDPAAWMRV
                     YFRHARSIHRTAARWLEECEAAGSSLFSQFRDWRSRLSNAEFTVSRESVLFKSPQLLA
                     QDTELVLRLFEFTARHGIRPAPDSEQRIAEHLSALEQHRSAPRPLWPRLEAILSLPNA
                     AIALRSMHECGVLNAMFTEWKEIDCLVLRDFHHRYTVDEHTLVSIQEIDGLRGNQDPL
                     RLRFAELLDEIERPEILRFALLFHDTGKATARGPHTEVSAALAEQALTRIAAPPDVRD
                     SVRFLIERHLDFSSVLNSRDLGEEGTARFLAGRVETVERLKYLTLVTYADISAVHPSA
                     MSPWRLDQLWRLYLIAYNELTLELDSDRIEAPASDSPEKAEFLDGFPRRYLLTHNAPE
                     IEAHYQIRKLSIERGVAVDVKRLNGVYSLTVVTRDRPFLFASVAGALAGFGMNILKAE
                     AFGNRHSEVLDVFIFEDPNRTLDLNPAEIDRLHFTVERVVLGRMDVKELLERRPRPAL
                     PSRRSRVEPRVSFDSVGSSRATLIEIVAQDRPGLLYDLARTISSAGGNIEVVLVDTEA
                     HKAIDVFYVTFGGGKLTSGAEIPLREALLSVCRT"
     gene            complement(21856..22194)
                     /locus_tag="VLE22_13040"
     CDS             complement(21856..22194)
                     /locus_tag="VLE22_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSB15378.1"
                     /translation="MKKIEAIIQPFKLDDVKDALKEIGIDGMTATEVRGHGRQKGHKE
                     VYRGREYQVDLLPKIKIEMVVPSARMEEVIRTIVTAARTGKIGDGKIFVYDVAEAVRI
                     RNDDRGDAAL"
     gene            complement(22208..23590)
                     /locus_tag="VLE22_13045"
     CDS             complement(22208..23590)
                     /locus_tag="VLE22_13045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSB15379.1"
                     /translation="MKLPTLYLPLLLPVLANAQGGQSMDEKLAALQAATQAAQSAGDN
                     AWMLMSAALVLMMTGPGLALFYGGLVRRKNVLGTMMQSFVLMALVTVLWAVVGYSMAF
                     GEGNAIVGDFRYLFLNGVGAEPNADYAATIPQQTFMVYQLMFAIITPALISGAFAERM
                     KFSAMLLFMTLWLLVVYFPMAHMVWGKGGLLNAFLGGRIPCFDFAGGTVVHITSGVSA
                     LVCALYLGKRRGYPHEAVKPHNLVLSFIGACLLWVGWFGFNAGSALGSSALATSAFVA
                     THFGAAAAALGWILVEWLKNGKPSVLGGISGSVAGLVAITPASGFVKPFPALVIGFAA
                     GVVCYFMVASVKQRFGYDDSLDAFGVHGAGGTLGALLTGVFATNLVNSALKDASGRPM
                     PLGLTDGNSGQLLNQLAGCAIAWMLAAVATLAILKLCDVLLGVRVADEHEVEGLDLSM
                     HGEEAYNYES"
BASE COUNT         4696 a         7157 c         7681 g         4222 t
ORIGIN      
        1 gcgcgaggcc cggacggagc ggcacaagcg cgtggcgagg ctgggaggcg caggaacggc
       61 cacgttgggc ggtagtgctg tcgctcgcag gctgatcgtt cgtggcgctt cgtccatcct
      121 aggtcgaggt agttggagaa ggcagcgcgg gcacagcggc acgcgcaacc cttcacttga
      181 tgttgatccg caccgtgttt acgttcttgc cgtcaacggt gagttccacg tccacttcgc
      241 cgctacctct gaggctgcgc cccacccaca ggttcacttc gtaatcttct tttggcgtgg
      301 ttgtgacgac aaggttttgc agttgccttg ccttcctgat caccaggagg taatagagca
      361 aacatacgaa gttcaactca cggcagctgt caactccttc tgcgacggtc taatagacat
      421 ctgctggtag gtgtctgctc cagaagcgcg gctaataatc ccacgccccc atcaccgtaa
      481 tcgtcacttt gtcggacgtg cgcatgtcat cgatttcgat ctgtactggc cattcgccgc
      541 ctgcaccggt tgggttgtga ggcacaacga cgttgatctg attcacgcca acgaattcgg
      601 gtgagaggcc gcaaaaagta acaagagcag gaatgccgtt tataaagacg gccggcgttg
      661 cgatcgttgt ccgcagctga tcgcgagatg ccgcaccatc ggcattggcc ggcgtcaccg
      721 ggccaaatcc tgttccgaga atcatgatga tttccccggc tctggccggg cgagcaacaa
      781 cgccgggaat agactcgtac tcgcccgcaa ccgatccatc cgcgttgatg gccacagctt
      841 ggcgcgagcg agatgagatt gtgaagatac cgggtgagac cggctcgact tgcgcacgaa
      901 atgcggcaga cgtacttcca ctcacggtca caacgatgtc agtgactccg ggttccacac
      961 gccagggaac ttggagatta atctggcttg gcgagacgaa gtgaagcggg gctgtgattc
     1021 cgctgaaagt gaccgagacg cctgcaatcg agtcaggcag gggaactgtc gaagctgtcg
     1081 ccgttgaagc agcgagtccg gttccgaaga gcgaaaccaa agatcctgga ctgagaggtt
     1141 ggtcctgtgc aaacgaagct gcgttcacgg cgccagtaat gaccggagct tggctgtagc
     1201 cgatggttgg cgtcagcgcg attgccagca taacgagtgc cttcatacaa cctccttatc
     1261 caggatttcc atcttggccc ttccgcgcac agccagccgt agggtaacca aggagaaggc
     1321 catagcgcac caatcgacac aattctctcg gatttccatc ttgggttccc cagcacgggt
     1381 tagcatatta tacatccagt tgatcccccg agttgcattt tttcgaggga accgccttcg
     1441 ccccgtggca aacgccactt tcgcctggcg caaaaccaaa accggcaagt cccatcattc
     1501 cagtaagttc cgggcgcaac cctccgtccc tcagcctcct tctcctgaga tttcgataca
     1561 aacttcctcc gtctaacccg tccccaccgg taaggcggca atccggcgca ccatcgcccc
     1621 gaacagccgc cgtttcagat agccctcggc cagcagtagc cagtattaaa ccggcgtgct
     1681 tgatcactcg gccgcgtgct tcctcgttcc agaacctgat ggttttggaa aggctcgacg
     1741 gtgctcaaga agccctttcg ctgccgcacg tctcatagcc tctgcgcaag ggggttttgg
     1801 aaaggctctg gcgcgtcgct cagcgcctcg aaggctctga ctaccgtacc gactccagcg
     1861 cccattctgc gggtaatggt gggccgatcc gttcccgctc aaactcgcca acgctgcacg
     1921 ccccagttgc cctccccagc ttgggggaga cctttttccc acccttacgg gatgtgcgga
     1981 ggcacgaagc gccgtagact tagctcatgc agaagaatca actgacgaag gacatcgcca
     2041 ttgccctcat tacgggagtc accctgttgt ggtgtgccgc tcccatgcag gctgacgaga
     2101 ccagcaaggc cgcaaaggcc gaggaactgc tgcaactgac gcagggcgac cagatgatga
     2161 agtcgtttct cgcgccgatg aaaggcgtac tgacggcgga tatgaacaag acggacatgc
     2221 ctgctgagaa gcgtgcgaag gctagtgaaa tgcaggcgaa gatcatgacg atgatcgccg
     2281 accgtctcag caaagccagg cctgcgctcg tgaaggccta caccgatact ttcacggaag
     2341 gggaaatcga cggcattttg gcattctaca aatcgcccgc tggacaagcc ttgcttcaga
     2401 gaatgccgca ggttatggag cgctcaatgc cggtgatcat gcagttgatg ggcgacctgc
     2461 aaccggaaat aaagaggatg acggaagaac tgaagtagat ggctctcgga aaggctctgg
     2521 cacggcattg gacgcacgat aggccctgac taccgttcca actcccgctc ccgtcctgcg
     2581 cgcgatttcc ggccaggaga gccgctcgtg gttccgcaga cgggcgactt ccgcccggtt
     2641 tagcatcgcc tcgccttctt tctcccgacc agcgccccga tccgttagcc aggatgggag
     2701 gactgaccat cgagcgctcg cagcatccgc tgcacggtca tggcgctggc accgaccgcc
     2761 cttgcgattt cgcgcaggct gcggccctcg cggaggagcc gcagcacctt gcgccggtca
     2821 actacgcggt gggggcggcc aaggcgcttg ccctcggcca cagcacgggc cagaccggcg
     2881 cgaacgcgtt actcgatcct atacggcaag taagaacgga acaagttact gaaggccgaa
     2941 tttgccgtgg ttggcctcta tgaacgtacc gacttccctc acaaccattc tggcctcttc
     3001 ctcttggatt ggcctatcct cgtgcatgac ctcatcccgg tgacgccaca gctcctcaaa
     3061 gtcaacagtc cgctttttgg gcaggtagac gccttcctgg aacagaagag cggcgcgcag
     3121 ccggggactc tccgcaagta caaacggcta atgggggcac tggagatctt cgcgcggcat
     3181 cggggcatag acgcggtctc tcgggtacgg ttggaggacc tggatgctta ccggaagggc
     3241 cgcaaggtca acgcactgac ctggacaaaa gagcttcagt tgcttcgaca gttctttgga
     3301 ttctgccagc ggcggaaatg gtgcgaggga aacccggcga aggatatgga gatgccatca
     3361 gagccgaagc cgtcaccaag ggagccgtat accgcggaag agattgcgcg gattctggca
     3421 gcctgtgaga ctttcggcaa ggggcaatac gagcgactga gggcgcgcgc ggcgatcctg
     3481 ttgatgcgtt actacggatt gcgcgtctcg gacgtcgcta cgcttgaacg agcgcgggtg
     3541 cgaagcggtc agctattcct ccatgccctc aagaacggcg ctccactgtg gctgcccctc
     3601 tatccagccg ttgagttcgc cctggaatgc ctccctgctc caaggggagc aactgacacg
     3661 cggtattttt tctggaccgg gctaggatcg cgcgacgggc aaatcaagac ctttgaccgg
     3721 acgctacagg cggtttttac tgagagtaga gtcgcgggcg cacacgctca ccgattccgc
     3781 cacacgctgg ccacggagat cctgtcaagc gggggcagca tcgaagacgc ggccaacatt
     3841 ctcggcgatt caccagcgat catccgtaag cattatctga agtggagtcc agcgtatcag
     3901 cagcgcacaa tggaggtctt ggcgcgggtt catggtacac ctgcggtaca cgaaaaaaga
     3961 gtgcccacaa atcctttgga atcaaaagat tgggctggtg ctggaggagg gggtcgaacc
     4021 ctcatgacca gtgaaggtcg cgggattttg agtcccgtgc gtctgccagt tccgccactc
     4081 cagcgatcgg caggtggggc gggcgcgccg cctcttcgag tatatcagcc tcaggcgagc
     4141 tgggacgcca gttcgcgggc gcgctcgaac agcatgcccg tctcgctgct acaccccagg
     4201 aagcgcatgc cgcgctcctt ccagaactgc gccagcttca gcgtacgggt ctgcgtgccc
     4261 ggcgccaccc cgcgttcgat acaggcgtcc cgcaccttct ccatcgcctc caccatcgtc
     4321 gggtgctgga attcgcccgg cacgccgagc gagatcgaaa ggtccgccgg gcctaccatc
     4381 acggcatcga ggcccggcac cgacagcagc tcgccacagg cctccaccgc gcgccgtgtc
     4441 tcgatctgga gcaccaccat cgtgttcccg ttgacgtgcc cgatcacctc ggggaagctc
     4501 gcgctctcgt aatcggtctg catcgccgta agcccgtagc ctcgtatgcc ggccggcgga
     4561 aacttcgtcc agctcacggc gcgctccagt aaagccggct cctccacgcg cgggaagata
     4621 acaccctgcg ccccgcagtc gagcgcgcgc gtgatgagcg aatactggag ctcggcgacg
     4681 cgggcgagcg ggcacagtcc cgcgaggttc gccacgcggc agacgtcctg caccgtctca
     4741 acgccgaacc cgccgtgctc ggtgtcgatg aacgcccagt gaaaaccggc ggccgccaaa
     4801 agcctggaga tctccgggca gcgaaactgg ccaaagcttg tcccaagctg aatttggcct
     4861 tccttgagtg cctgtttgac gcgatttttt gtcatgtgag cagttcagac aaccaggact
     4921 tgatggtccg ggcagtcaag ctgcacattg tcataaatcg cgtcgaggag ttcaagccca
     4981 tgcttggcca gaaacgggag gatcgagtag agccgttcct ggaggtgcct ctcgggatag
     5041 acgaggccgt acacgtaggc agcctcctcg gccgcccgct cgtcgcggcg cagcgattca
     5101 cgagccactt tgtgctccag cttggtgaac tggtgaacga tcttccggcg gcttgtgtcg
     5161 agcgcggccg ccagcgtcgg atcgaagcgc acgagttccg cccggagccg ctcgatgtgt
     5221 ttctcggtcg ccgccgacgc ctcccaaaga cagcggctta gtcccggagg cacgagccgg
     5281 gacgcgatcc gctccctcag cgccccctcg ccgtggtaga agtcgccaaa cgccagaccg
     5341 taacgcgaga tgagcctgtc gctgcgcgaa tcgaggagtg tgaagcccgc gcggggcaca
     5401 accaccggca tccgccccag aagcgtgcga tagagcacct gcgactgcgc aagataggcc
     5461 aactcagccg gccccacaac cgtagccgcc accggcaaca ggtaatcctg aacgacgggc
     5521 cgcaacagcg cgttcggaga gaggctctcc gcgcggccgg cgagttcgtc cgccgagatg
     5581 cggagtgtcc cggcgatgta gctgtcttcg gtccgccgca acggcagacg ccgtccaccc
     5641 tccaggagaa acaggagcga actttgcgac tccacatgaa cctgagcgtg ataccccgcg
     5701 gccgccagag cgctgttccg ctccagcacc gccggcacga ggtcgcctgc ggcctcgagt
     5761 gaccggcgca ggaccggcgc cgccagctca cgcaacgcgg gctgcatcgg atcgaagtac
     5821 acaagtccga acttctcgag cagccggcga agcagttcgt ggaacgccgc gccgaaactc
     5881 cggccctccg tgtaagtctg ctcgaccaat gcggcgacgt cgtctccgaa cgcaaacccc
     5941 gagagagtcc gccggagctc accggtcaac tcgccggaaa acggcgcctc tccgaccggt
     6001 ccgtccgtgc gggcaggcga tcgtgcttcg agccgcaccg ggcggtgcgc cgcgtcaaag
     6061 atccagcagt gatccacctc ggcgaaatcg tggtcctcgg tagccagcca gaaaaccgga
     6121 acggcgggca ttccctgctc ggtgagccgg gcagcgagct tcaccgcggt cagcgccttg
     6181 tagatcgtgt acgcgggacc ggagtacagc ccaacttgct gcccggccac caccaccacc
     6241 gttccggggt gggcgagccg gtcgagcgtc gggtggtcgc cgttctgctc cctaagggca
     6301 ctcaccagcg ccgtgcggcg ttcgtcgggg aaatcgaggc ggcttacggc ttcctggaaa
     6361 ctcgcaggct gacggggagg gagttcataa aagccggcaa ctttctcaaa atggtagaca
     6421 tagtcctcga acaaccggct ggtgtgggga agttccgtgt gacgcaggca gcgcgattcc
     6481 atcgggaagt ccaagggtag cagatgatcg atccttcccg aaagtcgcaa cggccgccgc
     6541 tactaagccg ccgagtgctc aacctcaagg tctctggcgt aaggagcggg ttccccttcg
     6601 ggcgcgtgca acaggttgta aagagaacgg tgcgcgggga tgctagactc gacaaaatcg
     6661 gcgccgagcg cggtgattac gcagtccccg ttattctccg ggcggatgaa tcctttctct
     6721 ttgaggaacc agaacgtgaa catcaggtgc tcgcgcggaa acgacatcat ttgttcgaac
     6781 tccaacagag ataagcccgg atcttcagga ttcgagcgcc ggcgcatgta gagcaaacaa
     6841 agcacgccca aacggcggtt agcctctccc tcgacaccgc cgatgaattc cttcaactcg
     6901 aacaccttaa gcggttcgct ccttcgccgg ttcaagcggg agtcgtactc ggctctccgc
     6961 tggggatcga tgagtgtacg gtacgcccgg ttgagttgca ggaacttctc gcagtccccg
     7021 gtttccgggt tgtcggggtg gaggcgcacg acgagcattc gatacacgcg ctggatcgtc
     7081 tccgtctcgg cgttgggact gatctgaagg aactcgtagt agtccgcaag atcttcacta
     7141 tccggaatgt cgtcgccacg cttgacgtca ttcatctcca gtccgatgac gaatttgccg
     7201 tcccggcgag tgcagtgtcg cactgacgcg gaccccgcca gaccgtactg gtccatgttg
     7261 atgaacacgt aagaattggg atctatccgt tcttccgtct cgatgcgcgc tcctttttcg
     7321 gagacgtcga gtccccgcgc ccgaatgaat cttgtgttgc cgaccttatc ctgccaggaa
     7381 agggagagct cgcaatcaac cggtttacgc cagtgccgcc gtgcctcatt ttgccgcata
     7441 tcttgtacct ccgtgttgac acccgccacc gcggtagcgc ggttcagcct gcccggtgcc
     7501 gttccgattc tatgagccgt agcgggtcct gcctatcgac caacaaacgg ccttcgtacc
     7561 agacctgcgc acgaccccgt tatggcactt tagtttcagc cgggagaaac ccggccggcc
     7621 gcgcagtcgt accggaaggg ccggttctgg gtcttttcga ctcgcgaacc agaaccctgg
     7681 tcctacgcct tgcggccttc tctcggcaaa agcagccgcg cgaccggata acgcccccag
     7741 caggcgtcgc tgggagcgta ccgcaggcac aaaggtaccg gtacaagatc gacgacggag
     7801 ctattctccg ggccgcgccg aagtcagtaa gatcgctcct gcagtgatgg ccaattgggt
     7861 cagccgcatc cggaaaaccg tgcgaacctg gcgagagggc aggcactctc tcgccgtagt
     7921 cgtatccatg ctttgcgtcg gtctttttct gctggcgatc tgccgggtct tctgggttcg
     7981 cgccgcccgc gtcgaacgct gtgtcgaact ggccaaccgg caactggaat tgtcccggga
     8041 attcctgtcg ctggtccgcc agccaaaagg tcgccctggc gactggagtg gaggggatcc
     8101 acttctcatc accgagttcg acaccgctct cgcattgctc gaggaccatt tcagccagga
     8161 ccgaggcgtg ctgcctctcc cgacacggct ctcacgaggg ctcgcagaag tccggcggct
     8221 gtggccggag atcaaggaac cattacggca agcggccgcg cgcaggggca ccgaagacgc
     8281 tcgccacctc gagccgaagg tgattgccat ggtcgaagcg tgggacggac tcggggatgc
     8341 gacgagacag cgaaggcttg cactccagca gtggctcatc tgcgtaatgg cggcaatcac
     8401 cgccgcggcg ctcatgctgc tttactccgg cgcttgggtc atcgcggctg gcggcacgcg
     8461 tgcccggcgg ttcgtggaag cgatcgtgcg tcacgcagcg aacggtccgt tcggggatcc
     8521 cagccgggcg gcggcccgga ccgacctcgc taagctgccg cggatgttca acggcctttc
     8581 cgaggcgatg ggcgcggtcc tcgaatacgt ggaggagaat tccagggccg tcgaagacat
     8641 cgcggaccgc cttccatctg gagtgctcgt cttcgctcag gatctttcgg tgcgttacgc
     8701 gaaccgtgcg ttcgtcgata gcctccgcct gacggagccg gacatccgcg gcatgagcct
     8761 ggggacggtt gtcgtgtgtg aaccgctgac gagatgggcc cgccatgtgc tggagaccag
     8821 aacctccgtg agcaacgtcc tggtggaggc gggcacgaac ggcgacaagc ggatactcag
     8881 ggcgcggttg gccgccttcc agccgcggaa taccgcggag gtgcagggac tgatcgtcac
     8941 cgaagactac acggaattcg attttctgcg cagcgcccgg gaagagtccg agcagaggta
     9001 ccggaggctg ctcgagagtt ccgccgaggc ggtggtcgtt gtgcgggagg acggcacgat
     9061 cctcgagatg aacaagctcg cggagacagc gttcgggtac tcgtgccgcg aggcggccgg
     9121 tctgccactg gtcgcgatcg ctcaggagag cggccgcgtg gtgcctgagc acacgctctc
     9181 ctcgcttctc cgctcgggca tgtggcaatt gcacggaacc accctcgaat acgagttccg
     9241 ccgccgcgac ggaacggtat ttcccggcga ggtgaggttg gcggcatgcc aggcagagac
     9301 aggccggctc tacttcgtga gtatccggga catcgcggac gtgaaacggt ccgagatcct
     9361 ggcgaaggac cggctcgaag tgatcgaaat gatcgctcga aaccaaccgc tggaagcggt
     9421 actggccggc ctggcgcaaa tgctcgaacg gcaggtgccg ggcgcacggt gcgcggtgat
     9481 gctgctgcgg gacaaccgtc ttcagccggc ggccgctccc aatatccccg agagtttcgt
     9541 gcgtgctctt tccgggatgc ggatcggacc ggacgccgcc tcctgcgggt cggtggcgtt
     9601 ccagggagaa ctgaccgtcg tcagcgacat cgcctccgag cccggctggg aagagtatcg
     9661 agaagcggcg ctaagccacg gactgcgcgc cgcctggtca gcgccaatcc actcgagcga
     9721 gggcacgatc gtcgggacgc ttgcgctcta ccacggggaa ccccggcagc cgaacagggg
     9781 gcagttggat ctgatgcaaa tggcctgccg gctggccgca gtgtccatcg aacaacggca
     9841 actgaacggg cgtctcaatt accaggccga acacgacgcg ttgacgggcc tccccaatcg
     9901 atttcacttc gagaactgga tgcaaaagca gctactgctt gcccgccggc accggcgccg
     9961 cgtgggcgta ctggtggttg atctcgatcg cttcaagctc atcaacgaca cgctcgggca
    10021 cgcggtcggc gacggtctgc tgcggcagat cgccggccgg ttgcgcgagc atgttcgcga
    10081 gacagacatg gtggctcggt ggggcggcga cgagtttgta gtcggactgc tggagcttcg
    10141 tgaaagccgc gacgccagcc tcgtagcgga aaaactgctg gacgcaatga ggaccgcgtt
    10201 cgaagtggag ggaaacgagc tcttcgcgac gttcaccatc ggaatcagca tctacccgcg
    10261 cgatggagcg gacttggacg cactcgttca aaacgcagac cgggcgatgt accgtagcaa
    10321 gaaccagggg gggaatgcct tccggtgcta ctccccggag atgggccgga ccgtcgccaa
    10381 gcacctggag ctggagacca gtctccgccg ggccctcgag cgtcacgaac tcctgctcca
    10441 ctaccagccg caattcgacc tccgaagcgg cgagttggtc ggagtggaag cactgcttcg
    10501 ctggagcagc catcggttcg gcttggttcc gccgtcggat ttcattccga ttgccgagga
    10561 aaccgggctg attgttccga tcggcgcctg ggcgctccga gaggcctgcc ggcagattcg
    10621 cgcctggcag gatgccgggc acacacctgt aaaggtggcg gtgaacgtgt cggtcctgca
    10681 gttcgcgaag gcggactttg tcgagatcgt cgcccgtgca ctggaggaga ccagcgtgct
    10741 cgctgaacta ctggaactgg agatgacaga gagcctgatc attcgcagtc tggaggaatc
    10801 cactgtccga atggcccggc tgcgatctct gggagtcggc ctcgccgtgg atgatttcgg
    10861 cacgggctac tcctctctgg cgtacctcca ccggctgccg atcgacaccc tgaaaatcga
    10921 tcagtcgttt gtcagggacc tcagtgtctc atcgagcacg cgggcgctgg tgcagtcgat
    10981 tgtgtctctc gccaagaacc tgggcatgcg gtcgacggcg gaaggcgtcg aaacgttttc
    11041 acagatgggc gcgttgcgaa gggcgggatg ctcccacgct caggggttct tcttcggcaa
    11101 gccgatggcg cccgaagcag tggctctgtt gatggaccgg caaaaggcag cccggacaaa
    11161 cggaccgccg ttccagatca tcacgccaat gccgcgagaa gacaccacca gcaaacaggc
    11221 acggctccat gtcctgccgc cgccggtgag gcgacccgca taccgtggtg gagagaacct
    11281 cacggttgtc tgagaatcct cccgaaagct gtcgattctg ttacctttag atgtgcccgt
    11341 gtcccagacc ggcgcgcgga gcgggtggcg gaagcgtcat catgagggac gatgcggagt
    11401 tcaaacacca cgctgacaat gcgatccata gtgtctacct cgccctgact gctgcctccg
    11461 acgactatgc gtacgacgtg agctatgaga gtggcgtcct taccgtcgag ataaagcggc
    11521 cgcgtgggag gcttgtcgtc gacgcgcatc ccgcagcacg gcagatccga atcttcagcc
    11581 cgtcaagaag ttacaagctg gactgggacg tcgtcgagaa cgaattcctg ctgtccggta
    11641 cgggggaaaa cctgaaagaa gccatggagg aagctctcag ccgccacctc aaggaagacg
    11701 tggcccttta gcaggacatg cccgcggtat acatctccta tcccttctgt cgacagaagt
    11761 gcacttactg taacttcgcc tcgggcgtgt ttccacgctc gctcgaagag cgctaccgca
    11821 aggctctgct ggccgagatc gggagtcaca tctggcgctg gcggccggag acgctgtacc
    11881 tcggcggagg tacgccgggc gacattgagg cggaagggct ggaggcgatc ctcgccgccg
    11941 tgcctggcca tccgtggctg gaggtgacca ttgaggcagc tcccggatcg atcgcgcggg
    12001 atcttgtgga cgtctggtgc cgcctcggcg tcaaccgggt aagcctgggc gtacagtcgt
    12061 tcatcggcgg cgaaattgcc cgcaccggaa ggaagcacac ggcccacacg gtgcgggacg
    12121 aggtggcgct gctgcgaaac ggcggtatca cgaacatcaa catcgacctc atcgccggac
    12181 tggccgggca gacggtcaag aactggagcg agtcgcttga ctggatcgag cggctggcgc
    12241 cgccgcacgt ctcggtctac atgttcgaac tggacgaaga cagccgcctc ggccgcgaga
    12301 tcctccaagg cggcgcgcgg tacggcgcgc gagacgtgcc ttccgacgac cggactgccg
    12361 agatgtacga aactgcggta gagcggctgc tggcgatggg cctggagcgg tacgagatct
    12421 ccaacttcgc cctgccgggg cacgaatcgc ggcacaacct gaagtactgg cggctggagc
    12481 cgtacacggg cttcggggcg gatgctcact cgttcgacgg cgacatgcgg cggcagaacg
    12541 tggaagcgcc gcaggattac accgagcggg tggagcgagg cgaatcgccc tgtatagggg
    12601 cggttcgggc ccaggcggag gaggaacgct tcttcgtggg tcttcggctc accgccggca
    12661 tccgtccgcg tccgaaggaa tggcagaggt tcgagcggcc tatccgccgg ttcctgggcg
    12721 agggactgct cgagaccgac ggcgaaacgc tgcggctgac atcgcgcggt attctcgttt
    12781 ccaatgaagt gttccaggag ttcgtcgaca tatgaatccc agcgaatgga ctgacctccg
    12841 cagtgacacg gtgactcggc ccacggccgc gatgcggcgc gcgatggcgg aggcggaggt
    12901 cggtgacgac gtctatggcg aagatccgac ggtgaaccgc ctggagcgcc gggcggccga
    12961 gatcttcggc aaagaggcgg gcctgttcgt gcccacgggc acgatgggca acacgatcgc
    13021 cgtcaaggtt cacacgaacc acggccagga ggttatctgc gaagcgcgct ctcacgtcct
    13081 tagctacgaa ctggcgatga tggcgtggtt ctccggttgt gtggcgaggg ccgttcgggc
    13141 cgaagacggc gtgctgcggt ggagccggat ccgtccggag atccgcttgc tcgggccgca
    13201 ctgggcgccg acggggctga tcgagatcga gaacacgcac aacatggccg gcggaacggt
    13261 gatgccggtg gaagcaatcg aggagatctg cgacggcgcg cacggcctcg gcctgaaggt
    13321 ccacatggat ggcgcgcgcg tgttcaacgc cgctgcgcat ctcgggcggc cggtcagcga
    13381 gatcacggcg aaggtggaca cggtgatgtt ctgtctgtcc aaggggctcg gcgcaccggt
    13441 gggctcgatg ctcgtcggca cggtggcgga catggcgcgc gggaggctgt accgcaagcg
    13501 gctcggcggc gggatgcggc aggtgggtgt gctggcggcg gcggggctga tcgctctcga
    13561 ggagatgcca aatcggcttg gcgaggatca cgagaacgcc cagtttctgg cggaggcgct
    13621 cgcgcggatc cccggaattc gcatcccgca cgaggtgcag acgaacatcg tgatcttcga
    13681 cgtcagcgga accgggctgg cgtcgtccga attcagcgcc cggctgaaga agcgcggagt
    13741 gctgatcaac gggatcaacc cgacgcagat gcgcctgctg acgcatttcg acgccaaccg
    13801 cacggcctgc gagcgcgcgc tggaggcaat cggacaggtg gccgccggag ccgcggtggc
    13861 aaaggaggca tagtgccatt cacgctgcaa ggaaaggcgg cgctggtgac gggcgccgga
    13921 cgcggtatcg ggcgggccat cgcggagcaa ctggcagcgg ctggagccgc cgtcatggtg
    13981 aacgacctcg acgacccggc agccgccgag actgaggcga tcatccgcaa tgcgggcggg
    14041 cgagcggagc ggctcgcggg cgacgtgacg cacccggatt ttccggaaag gctggtgaat
    14101 ggggcgctcc gcgctttcag ctcgatcgac atcatcgtca acaacgccgg ctacacctgg
    14161 gacaacgtaa tccagaagac gaccgacgaa cagttccagg ctatgctgga cgtccatatc
    14221 accgccccgt tccgcatctt gagggccgct tccggatgga tccgcgagac ggccaagcgt
    14281 gagatgagcg aggggcgccg cgtgatgcgc aaggtggtga acatcacgtc gatctcgggc
    14341 gtggacggca acgctgggca ggcgggttac tcctccggga aagcgggcgt gatcggactg
    14401 acgaagacgc tggccaagga atggggccgg tacaacgtaa cggtgaactg cgtcggcttc
    14461 gggatcatcg agacgcggct gacgcaaccg ctcggacagg agggcgcggc gatcgacatc
    14521 cgcgggcggc agatccctgt gggtgttcag ccggcgactt tggaagccct gcgggcgatg
    14581 tccccgctgg ggcggctggg gacggtagag gaggcagcgg gttcggtgct gttcttctgc
    14641 ttgccgctgt cggactacgt gaccggcgaa gtcctgctct gcagtggcgg cgtgcatttc
    14701 tgagcggcag atccctcttt tgcggtggac gtgggccagc ggcgaaactt ccggtctgtc
    14761 ccggaagtgg gccggcgcca atcggttcca gcggccggtt tcatccgcgc cacagcattg
    14821 accaaacaaa cccaattgcc cccatttgcg ggggctgccc gcctgtacct tatctcgatc
    14881 tcatcatatg agtgcggctg gtgagccgat cgctgttctg aagggcaaac ggctgaaacg
    14941 cgggggaatc gagcggtgga aaattggtga ccgggagagc ccgacagaac gagcgggtct
    15001 cggctggctt acgcaacgcc agaacgaagg gcaagcgtgt ggggcggccc aggcggattc
    15061 tcgaccggct ggagatggcc cggtcccgcg accagaatgg cctctcgcgg accgccattg
    15121 cccgcagagt gaggcgcctc acggatcgtc agcgccatgc ggtctgattc caaagtaggc
    15181 gtagccagca gctacagcga gccctagcca tgattgacca agatcggtta gcggctgtag
    15241 gactttggga tgtagcattt gaccgaccac ataggcagag agccccgttg cgacccatac
    15301 aataaggtac acgattgtta cccatttgac agtctgctgt gtcgtgacag aagccgtggg
    15361 ctcaagtgcg cgcgtcattg gggcttcgcc aggattcgtg actgcaagtt cagcaacaac
    15421 caaagccgag accaagcctc ccacaagcgt taatgcctga accataccgt ccgtgaagcc
    15481 gcttagaggg taactggtgc atcctgaggt ggacgcgcag ttgaccacaa gaatggcaaa
    15541 gctgaccgag tacacgtata ggccgagcaa aataacagcg agcacggccc cgatagcgat
    15601 tctcatcctg agcctcctcc ttagccagga tttccgcttt ggcttcctgg cccactgtgc
    15661 gcaggccgcg ttcgcggaga aatcctacaa ggaatggggt agtgctccca aaagggccgg
    15721 tgcagttcgg aaggagatag tcctccccgt gtgaatgtat gcggtagctt atggagttct
    15781 gatggcgttc acggggcagt tttcagacta cttcatccgt ttttctccgt caagccggag
    15841 tttgcgggcc gtggccccta ctatcagacc gtctggacat gttgggaaca tctcgcggtg
    15901 ctaggactca taaatcggag ccagttcgcg ttcagtcttc gtagatgcgg acgccttgga
    15961 cgacgcggtt gatgacgccg ccggggctgg ggttgtcggg gtcgaggccg aaccgaagac
    16021 tcagatggta tgccagcaac tggcagaaga cgatctcggc cggcgtgcgc aggtagtccg
    16081 gcaacgaact ggcttcggtg gtgacgactt catcgaaggc ggaagtgtcc gcgtccgcgg
    16141 gcgccaggcc cacgagccgg ccgatcttct tggcgcgcag ctcattgacg agatccattt
    16201 cgtaacgacg gcgcttcgga tcggaggaga tcaggcaaag caccagcgta tcggcgttca
    16261 cgaagctcat cggtccgtga cgaaggccga gataggtttc ggggatcgcc gggatccgcg
    16321 cggaggtgat ttcaagcacc ttgagcgtcg cctcgcgtgc cgcgccgaac agcggcgcgg
    16381 aacccagcgc cacgacgcga gcgggtggct cggcggcaag agccctcgcc gtcgcctcat
    16441 acttcccgat caccgtgcgg gcgccggcac aaagcacggg cagcgcctgc cccagttccg
    16501 ctggacggga aagcgctatg cctgccaaca cgaggttcga gaaagagctg gtcatcacaa
    16561 ggctgcggtc gttggagagc gggtcaagaa gcaccgcctc cacacccggc cagttggcga
    16621 gcttgccgtt ggggttgcag gtgagcgcga tgtggcggac ggagggcaac cgcttctgaa
    16681 ccttctcgac caccgcaacg gattcggggc tgtcgcccga gcgggcgagc gagacaatcg
    16741 tgccgaggct ggacagcgct tcctcaaagt cgaataagag ctcggtggag gcgatcgccc
    16801 gcgaaccggg ccaggccgac tcaatggcga tcgcggcgta ggcggacgtg cccgccccgg
    16861 tgatgaccgg gggcgacacc cgtccgagcg gggcctcgcg gacacgctcg aaggtgaccg
    16921 gccacgtgtc cggctgctgc ctgatttcag aaatggtgaa gcctccggca gagggaagtt
    16981 ccagggagga gttcaatgtg tcggccaagc aagcatgata cgaaagccaa ccggcggggt
    17041 tcaagtgaag cgcgagtgga tcggccgcgc taaagagaca tcaggtagct ggtgatcgcc
    17101 tgcatctgcc tcggggtgaa gcggtaggga ggcatgaccg tccccggtga aagcttctgc
    17161 ggatcgagga agtgctccac gacccattcg cgagtgcggc gctttgacag tccgttcagc
    17221 ggaggcccga ttttcatgcc gacgtcgttc acctggtggc aggcgctgca gccggcggcc
    17281 tggaacacga gcgcgccctg cacggcgaac gccggcgccg actccagcgc gtgcgcgttc
    17341 tggggcgtca gtttgagaag gaaggccgac agcgcgttga gttgcgcatc ggaaagctgg
    17401 atcggcggca tcgagctgcc gggcaccatt ttcgacggat tcttgaaatg ctgaatcatc
    17461 cagccagcgt tcttgcggat ggagatcctg gcgaggtccg gcccgacgtt tgcgccgccg
    17521 ttgcccacgc tgtgacagct cgtacagttc tcctgccgga agtagccaat gccggcgagt
    17581 tcctccgggg aaagctgccg ccattccccg actcctgcgg cgatttgtgt ctcctgaggt
    17641 ttgggtctgg tggccacggc ggcagcagtc aggccggccc agccgatggc ggccagcacg
    17701 acgacagcgg ccgcgtaggt ccgctgggtg agcttcatga ccttgccgcg gtcgacgaag
    17761 ggaaccagga acagaacgag gacagcgaac cccggcagca caacgctgcc aaccacttcc
    17821 agcggcccgg tgaagaactt caatgactgg aagaggaaca ggaagtacca gtcggggcgc
    17881 ggtatgtagg tggtgtcggt ggggtcggcg agccgatcga gagggacgcg caccgcgacc
    17941 gccaaaacga acaggacgat gaaggcggcg aagatcgcga tggtgtcctt cagcacctgc
    18001 tcggggaaga acttctttcc cggcgccgcg tcgccgggag ccggggccac gccgtgtttg
    18061 cgaactaggt aaacatggag cgcaatcagg aacatggtga gcggaggcag gaggaggacg
    18121 tgaagcgcgt agaatcgcgc gaacgtgacc acgccgatgc tgccttcacc accgagcagg
    18181 cggttgaggt atgggccggc gacgggcgcc ttgccagcga gctgcgtcgt caccacggta
    18241 ccccaatagg cacggttgtc ccacggcagc aggtagcccg tcagaccgta gccgagcgta
    18301 aggagaagca acgtcacgcc gacgatccag gtggcttccc gcggcttctt gtaggcgccg
    18361 aagaggaaga cctggatcat gtgaagcacg acgacgatga tcatcatgct ggcgccccag
    18421 tgatggagcc cacggatcag ggcgccgccg gtgagctcgg tgacgatgta cttcaggctg
    18481 ttgtaagcgt cgccgggcgt cggggcgtag ttgaaagcaa gcaggatgcc ggtgaacgcc
    18541 tgcacgagga agagaaacaa ggcaatgcta ccgaaaactt gcggccatcc gcttgaagcg
    18601 gggatgtcct catagaagaa gtttcggatg gcggtctcga gaccggtccg gtgttcgatc
    18661 cagctcagaa ccttctgctt caggcccatg tctatgccgg ttccgtggac cgttccgcag
    18721 cgccgagcag aagtctgccg ttctcgatcc tgacccgaaa ccgttccagc gggcgccggg
    18781 ccggaccgct gaggacccgt ccgtcgaggc cgaaagtcga actgtggcat ggacagagga
    18841 agttgttctt tcgctcgtcc cagtgatagg cgcagccgag gtgagtgcag attggcgaga
    18901 aggcaacgac gtcggcgtca gagaccttca ccacccaggc gctcgtcttt tcgctggtga
    18961 tcttccagcc gtccacacgg ttgcggcgga agacaagctc gttcgggaca ccgggctcga
    19021 gttgcgagac atctccggcc tccacccact cggactcttt acggggtttt ggcggcacca
    19081 gaagatatac ggctgccgga agtcccagcg ccaggcctat caatccccag agcgaataga
    19141 tgcaggtgac acagaaatac ctgcggtcag aaccgcgcgt ttcgttcatc gctttcccag
    19201 cagggtgctg aaaaacctgg cgtgcagccg ctccgtcagt tccttaggac cgctccctct
    19261 ggtcaccgct cggtgactgc gttaggaccg ccccctttgg tcgcggtttt ccaggcgcct
    19321 cctacaggta ttgtatttcc gggggtcccg atggggtgtt aaagatcggc accaagtttg
    19381 ttaattcaag tccggcaaac agacagaagg gcttcgcgaa gcggaatctc ggcgccggat
    19441 gtcagtttcc cgccgccgaa ggtcacatag aacacgtcga tcgccttgtg ggcttcggtg
    19501 tcgaccagca ccacctcgat gtttccaccc gcggaggaga tcgtgcgcgc caaatcgtag
    19561 aggagaccgg gacgatcctg ggcgacgatc tcgatcagcg tagctctgga ggagccaacg
    19621 gagtcgaagg agacgcgcgg ttcgacgcgc gagcgacggc tgggaagagc gggcctgggg
    19681 cgccgttcga gcagttcttt cacgtccatc ctgccgagaa ccacgcgctc gacggtgaag
    19741 tgaagacggt cgatttcggc gggattgagg tcgagggtcc ggttcggatc ttcgaatatg
    19801 aagacatcga gcacttcgct atggcggttg ccgaacgcct cggccttgag gatgttcatc
    19861 ccgaatccgg cgagggctcc ggcgaccgaa gcgaacagaa agggacgatc gcgagtcacc
    19921 acagtgagcg agtaaacgcc gttgagccgt ttcacgtcga cggcaacccc tcgctcgatc
    19981 gatagctttc ggatctgata gtgagcctcg atttcggggg cgttgtgagt gagaaggtat
    20041 cgtctcggga agccgtcgag aaattccgct ttctcggggg aatcggaggc cggcgcttcg
    20101 atgcggtcgc tgtcgagctc cagagtgagc tcgttgtaag cgataaggta gaggcgccac
    20161 aactgatcga gccgccaggg gctcatggcg gacggatgaa ccgcgctgat gtcggcgtag
    20221 gtgacaaggg tgaggtactt cagccgctcg acagtctcca cgcggccggc gagaaaccgg
    20281 gcggtgccct cttccccgag atcccgggag ttgagcaccg aagagaaatc gagatgccgt
    20341 tcgatgagaa agcggacgga atcccggaca tcgggcggcg ccgcgatgcg ggtgagggcc
    20401 tgttccgcaa gagcggctga aacctcggtg tgcgggccac gcgccgtggc ctttccggtg
    20461 tcgtgaaaca gcagggcgaa ccggagaatt tccggccgct cgatttcatc aaggagctcc
    20521 gcaaagcgaa gccgcagcgg gtcctggttt ccgcgcaggc cgtcgatttc ctggatcgag
    20581 acgagggtgt gctcgtcgac tgtgtagcga tggtgaaagt cgcggaggac gaggcagtcg
    20641 atctccttcc attccgtgaa catggcgttg agaacgccac attcgtgcat ggagcggagc
    20701 gcaatcgcgg cattcgggag ggacagaatt gcttcgagcc gcggccagag cggccgggga
    20761 gcgctccggt gttgttccag cgcggagagg tgttcggcga tgcgctgctc ggaatcgggc
    20821 gccggccgga tgccgtgacg cgcggtgaac tcgaagaggc gcagcacgag ttcggtatcc
    20881 tgtgccaaca gttggggcga tttgaaaagg acgctctccc gggatacggt gaactccgcg
    20941 ttggagagcc gcgagcgcca gtcacggaac tgcgagaaga gcgaactgcc ggccgcttcg
    21001 cactcctcga gccagcgcgc ggctgttcgg tggatggagc gggcgtggcg gaagtatacg
    21061 cgcatccaag ccgccggatc actcgcgggg gagaatggct gatcggcaag ctcctcctgg
    21121 gcgtcaaaac cgagcagatt gctgtctcgc ccggtcctgt aatgcaggaa acagcggacg
    21181 gaataaagga atgtcctggc gttcgcgagc tcgggaaacg gatccggctc cgaccggcgg
    21241 taagactggg cattgcgaag ctggctcagc cagcgggcta gctggtagtc ccggagcccg
    21301 cccggagcct ctttcacgtt aggttcgagg tgatagatcg tgtctgagta acgggcctgc
    21361 cggacgcggg tgagccggca taactggcgc gacagctcgt cccgacgtgc gctgaagaaa
    21421 cgcggcagtt tttcctccat ccgctcaaac aaggccggat cgccagccag gaaacgacgg
    21481 tccaggagac tgatgctcaa ctcgacgttg ccgtcgtgga cttcgcagca ctccgtggga
    21541 gttcgcacgg agtggccaag acgcagaccg gaatcccaga ggttttggag aaaacgcgag
    21601 acggcgtcgc gctgcccggc ctcactaagc tccaccggta cgagcagcag aaggtcaacg
    21661 tcggaatgcg gaaacagctc gcgccgtccg aagccgccca cggcgagcag tgccgcaccc
    21721 ccgggaagcg caggcgcgat gaattcatcg aaagatcgca cgaccgccgc ttcgaccata
    21781 gcggaccggc cggcgagaac ggggaacgcg tcaccatccc ggaagaacgc ggcgcgaagc
    21841 tcgtccgcct ccggactaga gtgcggcgtc gccacgatcg tcattccgga tccggacggc
    21901 ttcggcgacg tcgtagacaa agatcttgcc atcgccgatc ttgccggtgc gcgcggctgt
    21961 gacgattgtc cggatcactt cttccatgcg ggcggacggc actaccatct cgattttgat
    22021 cttcggtaga agatcgacct gatactcacg gccgcgatag acctccttat ggcccttctg
    22081 ccgaccgtga ccgcgaactt ccgtggcggt catgccgtcg ataccgattt ccttcagggc
    22141 gtccttgacg tcgtccagtt taaagggctg aatgatggct tcgatctttt tcatccgaag
    22201 gtcctcttta cgattcgtag ttgtaggctt cctcgccgtg catgctgagg tcgaggccct
    22261 cgacctcgtg ctcgtccgcg acgcggacgc cgaggagaac gtcgcagagt ttcaagatgg
    22321 cgagggtggc caccgcggca agcatccagg cgatggcgca gccggcgagc tgattgagaa
    22381 gctggccgga gttcccgtcg gtcaggccca ggggcatcgg acgtccggaa gcgtctttga
    22441 gtgcactgtt gaccaagttc gttgcgaaga cgccggtgag caacgcgccg agggttcctc
    22501 cggcaccgtg cacgccgaac gcatcgagcg aatcgtcgta gccgaacctc tgctttacgg
    22561 atgccaccat aaaatagcag accacgccgg cggcaaaacc gatcaccagc gccgggaatg
    22621 gttttacgaa gccggaagcc ggcgtaatcg ccaccaggcc ggcgacggag cccgagatgc
    22681 cgccgagcac gctgggcttt ccgttcttca gccattccac caaaatccag ccgagagccg
    22741 ccgccgcggc gccaaagtgc gtggcgacga aggcgctggt ggccagcgcg gatgatccca
    22801 gggcgctgcc ggcgttgaaa ccgaaccagc cgacccacag caggcacgct ccgataaagc
    22861 tcagcacgag gttgtgcggc ttcaccgcct cgtggggata cccgcggcgc ttgcccagat
    22921 agagtgcgca gacgagagcg gaaacgccag aggtgatgtg aacgacggtg ccgccagcga
    22981 aatcaaagca tgggattctg cccccgagaa atgcgttcag cagtccaccc ttgccccaaa
    23041 ccatgtgtgc catcggaaag tacaccacga gcagccaaag cgtcatgaac agcagcatcg
    23101 cgctgaactt catgcgttcg gcgaatgcgc cggagatgag ggccggcgtg atgatggcga
    23161 acatcaactg gtagaccatg aaggtctgct gcgggatggt ggccgcatag tcggcattgg
    23221 gttcggcgcc aacgccgttc aaaaacagat accgaaaatc cccaacgatg gcgttgccct
    23281 cgccaaacgc catgctgtag ccgacaacag cccaaagtac ggtcacaagg gccatgagga
    23341 cgaaactctg catcattgtg ccgagaacgt tctttctgcg aacgagaccg ccatagaaga
    23401 gagcgaggcc agggccggtc atcatcagca ctagcgcagc gctcatcaac atccaggcgt
    23461 tatcgccggc agactgggct gcctgggttg cggcctggag cgcggcgagc ttctcatcca
    23521 tactctgccc accttgcgca ttggccagaa caggcaggag cagaggcaga tacaacgtcg
    23581 gaagtttcat ccagcacctc tttgaaatag ttcgcagaac aaggcgttac ttactatctt
    23641 gcacgcacgg gccgggatct tctaacgaaa cgtttctatc ggggtaattg aaaaattcta
    23701 ttggcgcgtt ttcccaaacc agcctagaat gaggcaattt tgttctgccc ccgcga
//
LOCUS       DATIFQ010000278        24012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_282129, whole genome shotgun sequence.
ACCESSION   DATIFQ010000278 DATIFQ010000000
VERSION     DATIFQ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24012
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282129"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VLE22_13050"
     CDS             complement(<1..256)
                     /locus_tag="VLE22_13050"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HSB15380.1"
                     /translation="MKLIGRVESVWRYPVKSMRGEQLPEAFVGFGGIYGDRLFAFRSS
                     AAPKGFPYLTGREQEEMLLYQPRYRYPEKMSKPPNLAEAEA"
     gene            complement(344..1534)
                     /locus_tag="VLE22_13055"
     CDS             complement(344..1534)
                     /locus_tag="VLE22_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSB15381.1"
                     /translation="MIRLVVVLLFGGLLQGAEPLRVLFLGNSYTYFNNAPEIFAELAR
                     AALPDRQVEVQTVAIPGATLVSLWERSDARRILRSSKWDYVVLQDQSQLGDGLRDGKF
                     VVNAPILLHWGVRLFDAEVKRAGARTVLLLTWSRLSEPHQQGDLDYAYDSVARELGAI
                     LAPVGPAWQMVRQRSPGLELYAKDGSHPSPAGSYLLACVLLTSLFPEAARDLPFEVSG
                     HAVSTGGVVDTNRNTVLVALSAEQARSLQNSARDTVSDIRRNDGYLGALPPERPNVML
                     PSGEAMQPDQLAGVWTGDLTYYPSPAILDLTLRFEGGKCQGDVVIRVPDRNQRYDAPL
                     AECSLSGQELRFSVVTLPLPFLIDRFRGRITDGRLVGMVERTGRELTNSMSGTWALRR
                     RVAQ"
     gene            complement(1600..3387)
                     /locus_tag="VLE22_13060"
     CDS             complement(1600..3387)
                     /locus_tag="VLE22_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15382.1"
                     /translation="MNLAARRAAIVVVALHGILLLASLPDYFVSIDSGYHVSLARWYG
                     EHGTAWWDHINFGPGGRPNLQGPLMHMAIGYLGRALGGDGMDYVRANAILALAQWAAA
                     MFTVWFFARRYGGDWAALFAVTLLSGTLFASISFAVGVPSGWIFIFSAWAAWCFVEGR
                     LPLAILFTTAAIYTHLAGYAMAPLAVFVAAVLGRRWKALAIVGGATVLLTSPYTIHFL
                     RNLEWYRGRRGDEALMLAPLIYIPAAFGIVRLLRRPRENVFLIAWLAAPAPWIVQDYK
                     RFLLQAPLVLAVIAGVELAKFREWMTPRFRTAFTGGLVALAIVFPLTLPALALEAMWT
                     FGPRSPRMLDWNERKQLAEVIGRAGLNRRLVSVWNPTQCIAMAVFVPMSFEGGHWVEV
                     QPRDDPADRLSAGVKVYALPVPPDDPVLREMETQGFLTIHGGTWLNSIVTLNGPPPLE
                     TAARTVARVGSQEGGWLAKYSENNRMPGVGELLSVEATKRYRARAREQRTRTGRIQAA
                     ILVYAYALEKDYPDYARGARGAAGGFGEIANLLGDEATLDFRSEDDHRQLKANLAEWS
                     RAVRSFDTQVLPTLEVERTNDKVFHDYFR"
     gene            complement(3395..4381)
                     /locus_tag="VLE22_13065"
     CDS             complement(3395..4381)
                     /locus_tag="VLE22_13065"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HSB15383.1"
                     /translation="MMISLQDEFGQVETGEPVHFRNLTLLPLLRRKIETPDPSYLLLD
                     DAIAQGLARVTELGGGSVPELRFENRSDWPVLLLDGQELVGAKQNRVLNLTILAPARQ
                     TITIPVSCVEAGRWSMRSPDFKPAEHVMYSRGRASRAAQVTLSMHRTGARRSDQSGVW
                     DDIACKAERMGAQSPTQAMGAMYERHSISVEQYVRAFQCCDAQAGVVFAIDGRPLGLD
                     LFDHPATMRKLFPKLLRSYALDALDSSGAQCDAATMESLKELLARVASSPSFTQPALG
                     LGKDIRLSGLSVSGAALWAEERYIHVCAFAADGGPAPDQFGSHISRPTRRRI"
     gene            complement(4505..6130)
                     /locus_tag="VLE22_13070"
     CDS             complement(4505..6130)
                     /locus_tag="VLE22_13070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR family
                     protein"
                     /protein_id="HSB15384.1"
                     /translation="MMISGKTLFAFVDPKDPFMSGDIAGEDQPGPILSLVDARQFDTI
                     LLFHTPHTRENAGATRAEVARLYPQCRVTVTELPVSDPKDYSSLMGHLVRRVRAFVHT
                     QRGAKNHVCVSSGTAEMRAAWFLLAATGVLPAKLLQLGSPAEPLFGAANVREVQLDSS
                     DWSSLRDLVMPMEYFPSAEEAPPLAQRAKAPRLKSLFQRAAADAAMPKPTAALPPPPG
                     LDEALQELGIFIGSALMRAAAERAAIAAESDYPVLLFGETGTGKEMFAKLIHRMSERH
                     GRPMVAVNCASIPKDLAESHLFGHVKGAFTGAVADRKGVFEEADGSTLFLDEVGELPA
                     ESQAKLLRTLQDGVVQPVGSNHARTVDVRIVAATNKNLAGEIDAGRFRNDLYYRLEVV
                     QIKLPPLRKRESEISSLAATLLRRINHRRPKPRQLSKEAMLRLQQYDWPGNVRELSNV
                     LDRSVLFARSDVLGPDDLIITPAAATLDPFAGLPEPAPGFSLEEFLAQIRKQLILRAL
                     ERSNGNQSQAAAMLGLSKQAVHAFLKGENVKAD"
     gene            complement(6218..6634)
                     /locus_tag="VLE22_13075"
     CDS             complement(6218..6634)
                     /locus_tag="VLE22_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB15385.1"
                     /translation="MTGGFVADSSIGVAWVVPAQSSSSATELLDRILSGTRFFVPTLW
                     PFEVANALLALTRRGKLGDNHYRRAVDALRRLAPVVDEDGPRLALGQTSDLAARHRLS
                     VYDASYLELAIRRRLPLASRDTALISAAKKHELEVL"
     gene            complement(6631..6891)
                     /locus_tag="VLE22_13080"
     CDS             complement(6631..6891)
                     /locus_tag="VLE22_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HSB15386.1"
                     /translation="MDTVTAFEAKTRFGELLSRVANGEEIVITRHDKPVARLVPEGSA
                     SLARIQEAVHDINRLREEISLRPGFRRLSRRAIKDAVNEGRP"
     gene            7039..8865
                     /locus_tag="VLE22_13085"
     CDS             7039..8865
                     /locus_tag="VLE22_13085"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HSB15387.1"
                     /translation="MNLSHRCRMSGFAALVWICLAYPAPAQHVLRVTPSTVQWGYFAE
                     GKKPALTVKSGETVTMDTIVGIPDMLEELGATTDDSIREMKEMYAKVTDKGPGPHFLT
                     GPIAIEGALPGDVLEVEILEIRLRSPYGWMMIEPGAGILPGEFPYLRKKLVPLDQRNM
                     VAEFAPGVRLPVRPFFGNLGVAPPMGRIGSSAPGFHAGNLDNKWLVAGTKLFIPVQVP
                     GALFAAGDGHASQGDGEVCVTAVETNLTGVFRFTVRKDMKLRWPRAETPTHIIAMGLN
                     EDLDEAARHATTEMIDYLTSERGLSRDDAYMLTSAAVDLHVTQVVDGVKGVHAMLAKS
                     IFGAEPALKALVVDGQNNHAWRETTPVLKQLLEQTGLFRVSVATAPAKGGDMTAFKPD
                     FAAYNVVVLNYTGDPWTAATNAAFEKYVREGGGVVIFHAANNAFPDWKEFNEMIAVGG
                     WENRTTEKSGPMIRFRDGKVTTDSKPGRCGSHGARLPFQVAMRDRSHPITNGLPEVWM
                     HAADELYDSLCGPAKNVEVLATAHSDPANRGTGENEPMLMTIRYGKGRVFHTALGHDP
                     AAMQCAGFMTTLQRGAEWAATGKVTQKAPADFPTAEQVSLRK"
     gene            complement(8884..9288)
                     /locus_tag="VLE22_13090"
     CDS             complement(8884..9288)
                     /locus_tag="VLE22_13090"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSB15388.1"
                     /translation="MARRVDCYVDTSALIAFLDGSDSHHPLFRRLFSEPPPLVTSALV
                     VAEGHGWFLRRYDRERAIQFLAFIAELKPLTVLPFGDAELGKAARFVRKYRDQALTLA
                     DAHGLLIMDEGRIQTCWSTDRHLGLAGVTLVI"
     gene            complement(9272..9496)
                     /locus_tag="VLE22_13095"
     CDS             complement(9272..9496)
                     /locus_tag="VLE22_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HSB15389.1"
                     /translation="MIRTQVSLDEHEYDLAKREAAHQGISVAEFVRRAIRRTLPVEGH
                     APWMRFAGLVESGDRRSSQSVDEVVYGTKS"
     gene            complement(9643..9981)
                     /locus_tag="VLE22_13100"
     CDS             complement(9643..9981)
                     /locus_tag="VLE22_13100"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HSB15390.1"
                     /translation="MGSPKGRATADHVREGIAPYRRLKDSTVRTVLRRLEEKGYLTHK
                     IDGRTYIYEAAIPPQSVATHAVRQIIDRLCGGSVERLLVAMVENDVLEPHELERLARK
                     IALRKAGKGG"
     gene            complement(9985..13191)
                     /gene="carB"
                     /locus_tag="VLE22_13105"
     CDS             complement(9985..13191)
                     /gene="carB"
                     /locus_tag="VLE22_13105"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSB15391.1"
                     /translation="MPRRNDIHKVMIIGSGPIVIGQACEFDYSGTQACKALRAQGYEI
                     VLVNSNPATIMTDPGMADVTYIEPLNIKSMMQIIEEERPDALLPNLGGQSGLNLCAEL
                     ARNGTLEKYDVKVIGVQVDAIERGEDRIAFKETMARLGIEMPRSEPSYSVEEAERIAE
                     KLGYPVVIRLAYTLGGTGGGHVRNIEELRIVASRGIAASLIGQILVEESVLGWEELEL
                     EVVRDSKNQMITVCFIENVDAMGVHTGDSFCTAPMLTISEDLQRKLQEQSYRIVEAIQ
                     VIGGTNVQFAHDPKTGRVVVIEINPRTSRSSALASKATGFPIALVSAKLASGLTLDEI
                     PYWRSGTLDKYTPSGDYVVVKFARWAFEKFRGAEDRLGTQMRAVGEVMSIGKTYKEAL
                     QKSIRSLEIGRHGLGFAKDFHKRPLEELLERLAEPSSERQFLMYEALRKGASVDLLSA
                     KTHLKPWFIGQMKELVDLEEEILHFRGRPLPDDLLVRAKKDGFADRYLAQLLDLGEAA
                     VREQRGRLGVAEAWEPVPVSGVENAAYYYSTYNAPDTSVATSNPRKVMVLGGGPNRIG
                     QGIEFDYCCVHAAFALRDEGYESIMVNCNPETVSTDYDTSNKLYFEPLTIEDVLSIYK
                     KEKPAGVIVQFGGQTPLNLANALAASGVKILGTSPETIDLAEDRDRFRQIMEKLSFPM
                     PPSGMAASVDEALEIAGEIGYPLMVRPSYVLGGRGMEVVYDDEDLRAYVAKAVDVTPE
                     RPILIDKFLENAIEAEADAISDGTDVFVPAVMEHIELAGIHSGDSACVIPPVSIPTKH
                     IQTIEEYTRRIAMELNVVGLMNMQYAIANDTVYVLEANPRASRTVPLVSKVCGIQMAR
                     IATQLMLGRKLADLDLKPGVVQHYGVKEAVFPFNMFPEVDPLLGPEMRSTGEVLGLGQ
                     TFGEAFFKAQEGAQQTLPMVGTVLITVSEKDHSAVVEAARQFHEMGFRILATSGTAVC
                     LLDHGIPVLAVNKMHEGRPHIVDYVQKHEIQLIVNTPAGKQSQYDDSYIRKNAIKYKV
                     PYITTVAGALAAAKGIATRRQSGQDGVKPLQQYHAEVR"
     gene            13333..13647
                     /locus_tag="VLE22_13110"
     CDS             13333..13647
                     /locus_tag="VLE22_13110"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HSB15392.1"
                     /translation="MTKFLQALCLSLSLFAFAAAADQMTGYISDSKCGAAHTDGSEKS
                     VACVKACVKGGLAPVFVTEDNKVLKIDDKSKVTDFLGKKVEVTGSVEDDTLKVDTIKA
                     AD"
     gene            complement(13672..14766)
                     /locus_tag="VLE22_13115"
     CDS             complement(13672..14766)
                     /locus_tag="VLE22_13115"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSB15393.1"
                     /translation="MHIQDLETPALLIDLDIMERNMKRVADYAMRHNLRLRPHTKTHK
                     TPALGRRQLDLGATGLTIAKVGEAEVMLGAQPSDLLLAYPVFGRKKLERLMEVARQTR
                     VTVSLDNMLCASLLSEAASEGEVSIGVLAETDVGMGRVGVDPGTPLVDFVKTISRLPG
                     LMWEGVAFYPGHLRITGNEGLRAMEALGSTVESIRSDLRHEGFDPQVVSGGSTPTLFQ
                     SHLVRGMNEIRPGTYIFNDKNTVLAGACAWEDCAASVLATVVSTARPGQMIIDGGSKT
                     FSSDRLSGSDEVSFGRLMEDPAAVFRKMNEEHGYVDIRESSRKFKVGDQVRMIPNHVC
                     VVVNLHERMYGIRGERVEEVWKVEGRGKLQ"
     gene            complement(14770..15417)
                     /locus_tag="VLE22_13120"
     CDS             complement(14770..15417)
                     /locus_tag="VLE22_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15394.1"
                     /translation="MIFRAICIGMLSVVLCAAADKLPTGSASNDNIEVAATLHNGKEA
                     VQAVVGTDLGGYIVIVEVVVTPKGDKPLAVGPDDFILRSDKDGQRTGPFAPSQIAGKG
                     ALVVSARGGAGGMMMDESGPVWGGTMGRPRRLGGEGAAMGNTSVQTETSVHTTDKEKD
                     DPVLATLKAKVLPIKETAEPVSGLLYFLMEGKHKTKQLEMYYKGPAGNLSLRFRN"
     gene            15611..16789
                     /gene="dprA"
                     /locus_tag="VLE22_13125"
     CDS             15611..16789
                     /gene="dprA"
                     /locus_tag="VLE22_13125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HSB15395.1"
                     /translation="MLAAIQSREDELHWLALTLVPGLGARKAGQLIERFRSPQAVFRA
                     SKTELEACGIGGAVAQSISSGCSFEDAVAQQEKMLETGTRVVPISDPRFPEKLREIYD
                     PPVLLFVLGRVELLGSLMLGVVGTRRPTPYGVAVAERLAADLARAGLTIVSGMARGID
                     TSAHKGALAAGGDTVAVLGCGVDVVYPSENRKLAGDLAVKGLVLSEFPMGAPAYPQNF
                     PVRNRIISGMSVGVMVVEGAQYSGSAITARLAIDQGREVFAVPGNVTSKMSWGPNLLI
                     KQGAKLVQEWNDVVAELPVEERRRLIAAGQSRILSEGGGSGAETGDGRQASLDLGPSK
                     ELGKRILELLKVDSATHLDDLLERLEDGSSSETIAALFELEMLGLVRQLPGKNFVKVW
                     "
     gene            16853..19285
                     /gene="topA"
                     /locus_tag="VLE22_13130"
     CDS             16853..19285
                     /gene="topA"
                     /locus_tag="VLE22_13130"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HSB15396.1"
                     /translation="MSKSLVIVESPAKAKTIGKYLGKQFTVRASLGHVKDLPKKDLAV
                     DIDRNFEPTYEIIEGKRKLIAELKQAANGVDAVYLAADPDREGEAICYHLQEELEPKR
                     NGSPRIYRVMFNEITANAVRKAFEKPGIVNRNLVDAQQARRVLDRLVGYKISPLLWDK
                     VRRGLSAGRVQTVALRLIVDREREIRAFQKREYWTIDAHLLAKKPPVLVARLMRRNDE
                     QTEIPNQAAADNLVATLDGAQYVVKSVTTREKKRNPVPPFITSTLQQEAARKLRFSAK
                     RTMMIAQRLYEGVEIGKEGSVGLITYMRTDSTRVSTEALDEVRQFVRQRFGEPYLPSA
                     PNVYRSKKDAQDAHEAVRPTSVLRSPETVEKHLGEDEMKLYRLIWMRFIASQMMPAVF
                     DQTTVDITARGKDGAEYLFRETGSVMKFDGFLAVYEEGKDQKDEDDEELKHKLPVVHE
                     GDVLKFRSIHPEQHFTEPPPRYTEATLVKELEADGIGRPSTYASILSTIQEREYIKKE
                     GGRFAPTELGMVVTDLLLESFEDIFDVTYTARMEDELDEIEEGKIDWRAAMAEFYEKF
                     EVDLRNAERTMTDIKRMEKPTDLVCEKCGKPMVIKWGRHGSFIACTGYPECTNTRELN
                     VDLPDVDNADLTEQGDEEYCANCGRPMVLKKGRFGQFYACSGYPDCKTTKPIGGQQKK
                     ADVPLEEKCPKCGSNLALKHGRFGEFTACSNYPKCKYVKQKTIGVPCPNCSEGELIER
                     RSKRGKTFYGCNRYPECDFVAWAKPVAEKCPECGSPYLVEKWLKAGPQLQCPNAECKY
                     KRALAVPEPVAS"
     gene            19488..19820
                     /locus_tag="VLE22_13135"
     CDS             19488..19820
                     /locus_tag="VLE22_13135"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSB15397.1"
                     /translation="MPKKRTSETAPRSSSGDPAPAKTTARKPSVAKSQGNGAVQPVPR
                     LVRRKAPLAPVGQQGSPAAHSVPRDEVARLAYSYWESRGGRGGSPEEDWFRAEQEVLS
                     RIERPSAQ"
     gene            complement(19830..20462)
                     /gene="lexA"
                     /locus_tag="VLE22_13140"
     CDS             complement(19830..20462)
                     /gene="lexA"
                     /locus_tag="VLE22_13140"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HSB15398.1"
                     /translation="MPLTRRQKEVLDFIGNFIDENGYSPSFEEVASGLGLASLATVHK
                     HIRTLEAKNYLRRGHNQSRSLEVTPKFFQEQRRYREETSPAAGIPLVGRIAAGAPVEA
                     VAGRETLNFSDFLGSGESYALEVRGDSMIEDHICDGDLVLVERTAEVKDGEIVVALVR
                     GSETTLKRFYREPGDVARLQPANAGMAPIFVPLSDLRIQGRVLAVLRKYR"
     gene            20679..20933
                     /locus_tag="VLE22_13145"
     CDS             20679..20933
                     /locus_tag="VLE22_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15399.1"
                     /translation="MTFIWGLFEKRIADMRMDEKVFLLLIILACFCLVTYAMSLIVQI
                     VRKMEQQESPEQQAPASLRAGLLNSEALRRYDGPRYGHTD"
     gene            21202..22593
                     /locus_tag="VLE22_13150"
     CDS             21202..22593
                     /locus_tag="VLE22_13150"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB15400.1"
                     /translation="MPFSKQSRLSRRNLIAGAAAFAGFFKMTESDARAQARARQGFNR
                     NSAPSQLKITDMRSVLVASNYDYPIIRIDTNQGVYGLGEVRDAGREGTALVLKPHIIG
                     RNPLSIEPILDRLRIFCNHQRMGGGYSAVDMALHDIAGKVYGVPAWRLVGSKYRDKIR
                     IYCDTDQSKDPKVFAERLRKRAQQGFTFFKMDLGTSLVEDKPGAVDSRRVATEKGLQL
                     LGEYVQAVRDQIGWEAPLATDHFGALTVNDSIRYARAFERYNLAWAEDMIQIGHLGPG
                     GDAPKDWRGYKELKEATTTPIATGESLFGLEEGFKDLIDNRAIDIIHPDPLTSGAIRE
                     TKRIADYASMHGIETAIHFAGSPVGCLASVHMAATLKDMLAMENHAVDIPWWGDLVTG
                     PSKPIVDRGFIAVPDTPGLGIELNEDVVKEHIRKGGYFAPTPQYDDYILDHFRRGGPY
                     PHIDENGNPVVSQ"
     gene            complement(22614..22838)
                     /locus_tag="VLE22_13155"
     CDS             complement(22614..22838)
                     /locus_tag="VLE22_13155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSB15401.1"
                     /translation="MPIYEYRCDDCGTEFEKRVNRVADSTSVACPSCGEMHLTQKLST
                     FAPRVGGTRSDVPMCPSGGVCPTPGMCGRN"
     gene            complement(22852..>24012)
                     /gene="dacB"
                     /locus_tag="VLE22_13160"
     CDS             complement(22852..>24012)
                     /gene="dacB"
                     /locus_tag="VLE22_13160"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HSB15402.1"
                     /translation="GDPTMSGREYPYRKDARAGDPMRAIEELAEQVGLRGVRLVAGDI
                     AGDDTAYVWEPYPEGWAQDDSLWEYGAPVSALTFNDNAMTLRVRPGPEMGDPAQLRLT
                     PDTGYFAIDNRVATAAAAGERNVRIDRAPGSRQLRVWGAMPAKDRGVSVLVAADDPAL
                     YAATALREALIRRGVAVLGKAVARHRSASDVLDMKGGGEASAPSPGVELARRESLPLA
                     EILRVIDKVSQNLHAEIALREVGRARRNLGSRQAGLVEMRGFLQDADIAGSEYTMEDG
                     SGLSRLNLVTPEAVTKLLLAMSRSARAEVWNSLLPVGGEDGTLSDRFKGMRDGSRVRA
                     KTGTLGHVSALSGYVDSQSNGMLAFSILVNNYNAPSREIRAIIDKICSVIAR"
BASE COUNT         4645 a         7387 c         7549 g         4431 t
ORIGIN      
        1 tcgcttcggc ctccgcgagg ttcggtggtt tcgacatctt ttcgggatac cggtacctcg
       61 gctgatacag cagcatttct tcctgctccc gcccagtcag atagggaaat cctttcgggg
      121 cagccgaact gcgaaaggca aaaagacggt ccccgtagat accgccaaag ccaacaaatg
      181 cctctggcaa ttgctcaccc cgcatgctct tgaccggata gcgccacaca ctttcgaccc
      241 ttccgatgag tttcatttga gccccccctg actattctag tgccgttcca acttgatgca
      301 cctatgcgct aatgcgcggt ggggcgttga agagacgaag atctcattgc gccacccgac
      361 gccgcagagc ccacgttccg gacatgctgt tcgtgagctc tctgcctgtt cgctccacca
      421 ttccgacgag ccggccgtct gtaatccgcc ctctgaaacg atcgatcaga aagggaaggg
      481 gcagcgtaac gacagaaaag cgcagttcct gcccggagag ggagcactcc gctagcggag
      541 cgtcgtatcg ctggttgcgg tcgggcaccc taatcactac gtctccttga cacttgccac
      601 cctcgaaccg cagcgtcaga tccaggatgg ccgggctagg gtaatacgtc aggtcgccgg
      661 tccagacgcc tgccaattga tcgggttgca tcgcttcgcc gctcggcagc atgacgttcg
      721 gccgctcagg cgggagagcc cccaggtaac cgtcattccg cctgatgtcg ctaacagtgt
      781 ccctggccga gttctgaaga ctcctggctt gctcggccga caatgccacc aagactgtgt
      841 tccggttcgt atcgacaacg ccaccagtgc tcacggcatg ccctgatacc tcgaaaggaa
      901 gatcgcgggc agcctctgga aacagcgacg tcaagaggac gcaagccagc aaataggagc
      961 cggcaggcga tggatgggaa ccatcctttg catacaactc caggccaggg cttcgctgcc
     1021 gcaccatttg ccaggccgga cctaccggtg cgagtatcgc cccgagttcg cgggcgacgg
     1081 aatcgtaggc gtagtccagg tccccctgct gatgcggttc ggacaggcgc gaccacgtca
     1141 gcaaaagcac ggtgcgcgcc ccagcccgct tcacttccgc atcgaacagc cggacgcccc
     1201 agtgaagcag aatcggagcg ttcacaacaa acttgccatc gcgcaggccg tctcccagtt
     1261 ggctttgatc ctgcagcacg acgtagtccc acttcgacga acgaaggatt cggcgggcgt
     1321 cagagcgctc ccacaaagaa accagggtcg cgccgggaat cgcaaccgtt tgtacttcca
     1381 cctgccggtc aggaagcgcc gcacgcgcca actcggcgaa aatctcgggc gcattgttga
     1441 aataggtgta gctgttgccc aggaagagaa ccctcagcgg ttccgcaccc tgaagcaatc
     1501 ctccgaagag cagcacgacc accaagcgga tcatgcctca ttctatgctc acggcgttcg
     1561 cgggactccg ccggaatgaa gccgtagagc ccgcgcatgc taccggaagt agtcgtgaaa
     1621 gaccttgtcg ttggtccgct ccacttcgag ggtcggcagc acctgcgtgt cgaaactgcg
     1681 aaccgcgcgc gaccattcgg caaggttggc tttgagctgg cggtgatcgt cctcgctccg
     1741 aaagtccagt gtcgcctcgt cgcccagcag gttcgcgatt tcgccgaaac cacccgccgc
     1801 gccccgtgcg ccccgcgcat agtccgggta atccttctcc agggcatacg catagaccag
     1861 aatggcggcc tggatgcgtc ccgtgcgtgt ccgctgctca cgggctcggg cgcgatagcg
     1921 tttggtcgcc tcgaccgaga gcaactcccc aacgcccggc atgcggttgt tctccgaata
     1981 cttcgccagc caaccccctt cctgagaacc gacgcgcgcc acagtgcggg ccgctgtctc
     2041 tagtggcggc ggcccgttca acgtgacgat ggagttcagc caggtgccgc cgtggatggt
     2101 gagaaagccc tgagtctcca tctcgcgcaa caccggatca tccggcggca cagggagcgc
     2161 gtaaaccttc acgcccgcag agagccggtc cgccggatcg tcgcgcggct gcacctccac
     2221 ccagtggcca ccctcaaatg acatgggaac gaacacagcc atcgcgatgc actgcgtcgg
     2281 gttccagacg ctgaccagcc ggcggttcag tcccgcgcgg ccgatcactt ccgcaagttg
     2341 ctttctctcg ttccagtcga gcatgcgtgg gcttcgcggg ccgaaggtcc acattgcctc
     2401 taaggccaaa gccgggagcg tcaggggaaa gacgatggcg agcgcaacca gtccgcccgt
     2461 aaacgccgtg cggaagcggg gcgtcatcca ttcgcgaaac tttgcgagtt cgacacccgc
     2521 aatcaccgcc agcaccaatg gcgcctgcaa caggaaacgt ttgtagtcct gcacgatcca
     2581 tggggccgga gcggccagcc aggcgatcaa gaatacgttc tcgcgcggac ggcgcagcag
     2641 ccggacgatc ccgaacgccg ccgggatgta aatgagcggg gccaacatca gcgcctcgtc
     2701 gccacgcctg ccgcgatacc actccaggtt gcggaggaaa tggattgtat acgggctggt
     2761 gagaagcacc gtcgcgccgc caacgatggc gagcgccttc cagcgccggc cgagcactgc
     2821 ggcaacgaat accgcgagcg gcgccatcgc atagcccgcc aggtgcgtgt agatcgcggc
     2881 cgtcgtgaag aggatcgcaa gcggcagccg gccttcgacg aagcaccacg ccgcccaggc
     2941 cgagaagatg aaaatccagc ccgaaggcac tccgaccgca aacgagatcg acgcgaacag
     3001 cgtgcccgag aggagtgtaa ccgcgaaaag cgccgcccag tcgccgccgt agcggcgtgc
     3061 gaagaaccac acggtgaaca tcgcggcggc ccactgtgcc agggccagga tcgcgttggc
     3121 gcgtacataa tccatgccgt ctccgccaag cgcgcgaccc aggtagccga tcgccatgtg
     3181 catgagcggg ccttgcagat tcggccggcc accgggcccg aagttgatgt ggtcccacca
     3241 ggccgtgccg tgctcaccgt accagcgagc gagcgagacg tggtagccgg agtcgatgct
     3301 gacgaagtaa tccggcagcg acgcgagaag caggatgcca tgcaaggcga cgaccactat
     3361 ggccgcgcgg cgggccgcca agttcatgga cctatcatat ccgccggcgg gtcgggcggc
     3421 tgatatggga gccgaattga tcgggcgccg gacctccgtc cgcagcaaaa gcgcagacgt
     3481 ggatgtagcg ctcctccgcc cacagcgcgg cgcccgagac tgagaggccg ctcagccgga
     3541 tgtccttgcc gagcccgagc gccggttggg tgaaactcgg cgacgaagcc acccgggcaa
     3601 ggagttcctt cagcgactcc atggtggccg catcgcattg tgcgccggac gaatccagag
     3661 cgtcgagcgc gtagctgcgt agcagcttcg ggaacaactt ccgcatcgtt gccgggtgat
     3721 cgaacaggtc gaggccgagc ggacggccat cgatggcgaa taccacgccc gcctgcgcgt
     3781 cacagcactg gaacgctcgt acgtactgct ccacggagat tgagtggcgc tcgtacatcg
     3841 ctcccatcgc ctgcgtcggc gactgggcgc ccatccgttc ggctttgcaa gcgatgtcgt
     3901 cccaaacgcc ggactgatcg gagcggcgcg ccccggtccg gtgcatcgac aaggtgacct
     3961 gcgccgcccg tgacgcccgg ccacgcgagt acatcacgtg ttcggccggc ttgaagtccg
     4021 ggcttcgcat ggaccaccgc cctgcctcga cacacgaaac cgggatggtg atggtctgcc
     4081 tggctggagc gagaatcgtc aggttcagca cgcggttctg tttcgcgcct acgagttcct
     4141 ggccgtcgag caagagcacg ggccagtcgg agcggttctc gaaccgcagt tcgggcacgg
     4201 agcctccgcc cagttcggtg acacgagcga ggccctgcgc gatcgcgtcg tccagcagga
     4261 gatagctggg gtccggcgtc tcgattttcc gacgcagaag cggcagcagg gtaaggttcc
     4321 ggaagtggac cggctcgccg gtttccacct ggccgaattc gtcttgaagg gaaatcatca
     4381 tgagctcctt gggcaggttg cccgaggagg taaatgcacg cggagtgaca ttccgacggt
     4441 gctttgaaat caatgatgta cattgcgaga gacggctccc gcgtcaagcc gggtagaccc
     4501 ggcgtcaatc cgccttgacg ttctcacctt tcaagaaggc gtggactgcc tgcttggaaa
     4561 ggccgagcat cgctgccgcc tggctctggt tgccgttact tctttcgagc gcccgcagga
     4621 tgagctgctt gcggatctgg gcgaggaact cttcgagcga gaagccggga gccggttcgg
     4681 gcaggccggc gaaagggtcc agggtggccg ccgcgggtgt gatgatgagg tcgtcggggc
     4741 cgagcacgtc cgagcgggca aagagcaccg agcgatcgag gacattggag agctcgcgca
     4801 cgttaccggg ccagtcgtac tgctgaaggc gcagcatggc ctccttcgaa agctggcgcg
     4861 gcttcggccg gcgatggttg attcgccgga gcagcgtggc cgcaagggac gagatctccg
     4921 actcccgctt ccggagcgga ggaagcttga tttgaacgac ctcgagacgg tagtacagat
     4981 cgttccggaa gcgcccggca tcgatctctc cggcgaggtt cttattggtc gcagccacga
     5041 tgcggacgtc gaccgttcgc gcgtggttcg atcctacggg ctggacaacg ccgtcctgga
     5101 gcgtccgcag cagcttcgcc tggctttcgg cgggcaattc tccgacctcg tcgaggaaca
     5161 gtgtgctgcc gtcggcctcc tcgaagacac ccttgcggtc cgccacggcg ccggtgaaag
     5221 cgcctttgac gtggccgaag aggtggcttt cggcgaggtc ctttgggatg gaggcgcagt
     5281 tgacggccac catcgggcgg ccgtgccgtt cgctcattcg gtggatcagc ttcgcgaaca
     5341 tctccttacc ggtgcccgtc tccccgaaga gcagcaccgg gtagtcgctc tcggccgcga
     5401 tggcggcacg ctcggcggcg gcacgcatca acgccgatcc gatgaagatg ccgagctcct
     5461 ggagcgcctc gtccagccct ggaggtggag gcagcgctgc ggtcggtttg ggcatcgcgg
     5521 cgtccgcagc cgctcgctgg aacaggctct tgagacgggg agccttggcc ctctgcgcga
     5581 ggggcggcgc ctcctccgct gacgggaagt actccatcgg catgacgagg tcgcgcaggc
     5641 tcgaccagtc gctcgaatcg agttgcacct cgcggacgtt ggcggctccg aacagaggtt
     5701 cagcgggaga gccgagctgg agcagtttcg cgggcagcac accggttgcc gcaagaagga
     5761 accacgccgc gcgcatctcc gcggtgccac tcgagacgca cacatggttt tttgcgccgc
     5821 gttgtgtgtg cacgaatgcg cgcacccggc gcacgaggtg tcccatgagc gaggagtaat
     5881 ccttggggtc agagacaggg agctccgtga ccgtgacgcg gcattgcggg tagagccggg
     5941 cgacctcggc gcgggtagcg ccggcgtttt cgcgggtgtg cggtgtgtgg aagagcagga
     6001 tggtgtcaaa ctgccgggcg tccacgagcg agaggattgg accgggttga tcctcgccgg
     6061 cgatgtcgcc ggacatgaac ggatccttcg gatcgacgaa ggcgaatagc gtcttgccgc
     6121 tgatcatcat aacctgaggc aattctacag ccacggtgcg agatgcgccg ggtctctagg
     6181 atgcgtgagg cgctgctgaa aagcgccagc gttcctttca gaggacttcg agttcgtgtt
     6241 tctttgccgc ggagatgaga gcggtgtccc tcgaagccag cgggagccgg cgccggatgg
     6301 ccagctccag atagcttgca tcgtagacag aaagacgatg acgcgcagcg aggtcagacg
     6361 tttgtcccaa ggcgagacgc gggccgtctt catcgaccac cggcgccaat cggcgaagcg
     6421 cgtccaccgc gcgccggtag tgattgtctc caagctttcc gcgccgcgtg agcgcaagga
     6481 gagcgttcgc aacttcaaaa ggccagaggg taggcacgaa aaagcgtgtc ccgctgagaa
     6541 ttcggtcgag cagttcagtt gcagaactgc tggattgcgc cgggaccacc caggcgacac
     6601 cgatggagga gtcagcgacg aagccgccag tcacggcctg ccctcattga cggcgtcttt
     6661 gatcgcgcgt cggctgagtc ttcggaagcc gggccggagg ctaatctcct cacgcagccg
     6721 gttaatgtcg tgaacggcct cctgtatccg cgctaaggac gcactacctt ccgggacgag
     6781 ccgggcgact ggtttgtcgt gccgggtgat tacgatctct tcaccgttgg cgacgcggct
     6841 tagcaactca ccaaagcggg tcttggcctc aaaagcggtt acggtatcca taatttgact
     6901 agctattgtc tagtctaaca tgaccgggtc tctgccgcgc ttcaaggcca aggcacgttt
     6961 ccgcgcccgc ttctcgccga ccgagggcgc aagtgatact ctcggtttgt tattccgaca
     7021 aaggagtccg aggaatctat gaatctcagt catcgttgca gaatgtctgg tttcgccgca
     7081 ctggtgtgga tctgccttgc ttatccggca cctgcgcagc acgtgctccg cgtgacgccg
     7141 tccaccgtcc aatggggtta tttcgcggag ggaaagaagc cggcgctgac ggtaaagtca
     7201 ggcgagactg tgacgatgga cacgatcgtt ggcatcccgg acatgctgga ggaactgggc
     7261 gcgactacgg acgattcgat ccgtgagatg aaggagatgt acgccaaggt gacggataaa
     7321 gggcctgggc cacactttct cacggggccg atcgccatcg agggcgcgct gccgggtgac
     7381 gtactcgagg tggaaatcct ggagatccgc cttcgctctc cttacggatg gatgatgatc
     7441 gagccaggcg cgggcattct tccgggggag ttcccgtatc tgagaaagaa gctggtgccg
     7501 ctcgaccagc ggaacatggt ggcagagttc gcaccgggcg tccgccttcc ggtccgccct
     7561 ttcttcggga atctgggcgt ggctccgccg atgggacgaa tcggcagttc cgcgccgggc
     7621 tttcacgccg gcaacctcga caacaaatgg ctggtagccg ggacgaagct gttcatcccg
     7681 gtgcaggtgc caggagcgct gttcgcggcg ggtgacgggc atgcatccca aggcgacggc
     7741 gaggtgtgcg tcacggccgt tgagaccaac ctcaccggag tgttccgctt caccgtgcgg
     7801 aaggacatga agctgcgatg gccccgagcc gagacgccaa cgcacatcat cgcgatggga
     7861 ctgaacgaag atctggatga ggcggcgcgg cacgccacga ccgagatgat cgactatctg
     7921 acgtcggaac gcggcttgtc gagggacgac gcctatatgc tcaccagtgc cgcggtggat
     7981 ctgcatgtca ctcaggtcgt ggacggcgtg aagggcgtac acgcgatgct ggccaagtcg
     8041 attttcggag cggaacccgc gctgaaggcg ctggtcgtcg acgggcagaa caatcacgcc
     8101 tggcgggaga cgacgcccgt gctgaagcaa cttctggagc agacggggct tttccgggtc
     8161 tcggtggcca cagcgcccgc gaagggcggc gacatgaccg ctttcaagcc ggacttcgcc
     8221 gcgtacaacg tggtggtgtt gaactacacc ggcgatccct ggacggcggc gacaaacgca
     8281 gcattcgaga agtacgtccg ggagggcggg ggagtggtga tcttccatgc cgcgaacaac
     8341 gcgtttccgg actggaagga gttcaacgag atgatcgccg tcggcgggtg ggagaatcgg
     8401 accaccgaga agtccggtcc catgatccgg tttcgggatg gcaaagtgac cacggacagc
     8461 aagccggggc ggtgcggcag tcacggtgcg cggctgcctt ttcaggtcgc gatgagagac
     8521 cggagccacc cgatcacgaa tggcctgccg gaggtctgga tgcatgcggc ggacgagttg
     8581 tacgacagcc tctgcgggcc ggcgaagaac gtggaggtac tggcgacggc gcactccgat
     8641 ccggcaaacc ggggcacggg agagaacgag ccgatgctga tgacgatccg ctacggcaag
     8701 ggcagggtgt tccacacggc gctaggacac gacccggcgg cgatgcagtg cgccggtttc
     8761 atgacgactc tccagcgcgg ggccgagtgg gcggccacgg gcaaggtgac gcagaaggcg
     8821 ccggcggact tcccgacggc ggagcaggtc agcttgagga agtgacaggg gcggcgaccg
     8881 gcctcagatc accagggtta cgccagccaa cccgaggtgg cggtcggtcg accagcaggt
     8941 ttggattctt ccctcgtcca tgatcaggag gccgtgggcg tccgccagcg tcaacgcctg
     9001 atcgcggtat ttcctcacaa agcgggccgc ttttccgagt tccgcgtcac cgaacggaag
     9061 gacggtgagc ggcttcaatt ccgcaatgaa cgccaggaac tggatcgccc tttcgcggtc
     9121 gtaccggcgg agaaaccaac catgaccctc ggccacgacc agggcggaag tcaccagggg
     9181 cggcggttcg gaaaacagtc gtcggaacag agggtggtga ctatccgaac catccagaaa
     9241 ggctatcaag gccgaggtgt cgacgtaaca gtcaactctt cgtgccatag acgacctcgt
     9301 cgaccgactg gctcgaacgc ctgtcgccgg attccacgag gcccgcgaaa cgcatccagg
     9361 gggcgtgtcc ctcgaccgga agcgtccggc gtatagcgcg acgcacgaac tccgcgacgg
     9421 aaataccctg gtgggccgct tcccgcttgg ctaggtcgta ctcgtgttca tccagggaaa
     9481 cttgcgtccg gatcatgtta tcaccatttg aaactatgat aacacgaggt cgaccgctcc
     9541 cactaccgcc ggtgagactg ctcaagttgc tggcgccgcc agtccgagga ggaaacggtt
     9601 cgtgtttcca gacggagggc aggagatctc cgagcggcat cactagcctc ctttgccggc
     9661 ttttcgaagc gcgatcttcc gagccagcct ttccaactcg tgcggttcga gcacgtcatt
     9721 ctcgaccatg gcgacgagca aacgctcgac ggagcctccg cagagacggt cgatgatctg
     9781 gcggactgca tgcgtggcga cgctctgcgg agggatggcc gcttcgtaga tgtaggtccg
     9841 gccgtcgatc ttgtgtgtta ggtatccctt ttcctccagg cggcggagaa cggtgcggac
     9901 ggtcgagtcc ttcaggcggc gatacggagc gatgccctcg cgcacgtgat cggctgtggc
     9961 gcggcccttg gggctgccca cccgctaccg cacttccgcg tggtattgct ggagcggttt
    10021 gacgccgtct tggccggact ggcggcgggt ggcgatgccc ttcgcggcgg ccagcgcgcc
    10081 ggcgacggtg gtgatgtatg gcaccttgta cttgatcgcg ttcttacgga tgtaagagtc
    10141 gtcgtactgg ctttgcttgc ccgccggtgt gttcacgatg agctggatct cgtgtttctg
    10201 cacgtagtca acgatgtgcg gccggccctc gtgcattttg ttcacggcca gtactggaat
    10261 tccgtggtcg aggaggcaca ccgccgttcc cgacgttgcc agtatgcgga agcccatctc
    10321 gtggaactga cgcgccgctt ccaccaccgc cgaatggtcc ttttcggaga ccgtgatcag
    10381 cactgtgcct accatcggca gtgtctgctg agcgccctcc tgggctttga agaaggcctc
    10441 gccgaacgtc tgtcccagac cgagcacctc gccggtggac cgcatttccg ggccgagcag
    10501 cggatccacc tcagggaaca tgttgaacgg aaagaccgct tccttgacgc cgtagtgctg
    10561 gactactccg ggcttcaggt ccaggtccgc cagcttccgg ccgagcatca actgcgtggc
    10621 gatgcgcgcc atctggatgc cgcacacctt cgacaccagc ggcaccgtcc tcgatgcgcg
    10681 cggattggcc tccagcacgt agaccgtgtc gttcgcgata gcgtactgca tgttcatcag
    10741 cccgacgacg ttcaactcca tcgcgatccg ccgggtgtac tcctcgatcg tctggatgtg
    10801 cttggtcggg atactcacgg gcgggatgac gcaggccgaa tcacccgagt ggatgcccgc
    10861 gagttcgatg tgctccatca cagcgggcac gaagacgtcc gtaccgtcgg agatcgcatc
    10921 cgcctcggct tcgatcgcgt tctcgaggaa cttgtcgatg agaatcggtc gctccggcgt
    10981 cacgtccacg gccttggcga cgtacgcgcg caggtcttcg tcgtcataaa cgacttccat
    11041 gccgcgtccg ccgagcacat aagacggccg gaccatcaac ggatagccga tttcgccggc
    11101 gatctccagc gcttcgtcga ccgaagccgc catgccggag ggcggcattg ggaagctgag
    11161 tttctccatg atctggcgga aacggtcgcg gtcttcggcg aggtcgatcg tctccgggct
    11221 cgttcccagg atcttcacgc cggacgccgc aagcgcgttg gccaggttca gcggcgtctg
    11281 cccgccgaac tgcacgatca cgcccgcggg tttctccttt ttgtagatgc tgaggacatc
    11341 ctcgatcgtc agcggctcga agtagagctt gttggaggtg tcatagtcgg tcgagacagt
    11401 ctccgggttg cagttgacca tgatcgactc ataaccctcg tcccgcaggg cgaacgcagc
    11461 gtggacgcag caatagtcga actcgatgcc ctggccgatg cggttcggcc cgccgcccag
    11521 gaccatcacc ttgcgcgggt tcgaagtggc gacggaggtg tccggagcgt tgtaggttga
    11581 gtagtagtag gccgcgtttt cgacgccgct caccggcacc ggctcccacg cttcggctac
    11641 accgagccgc ccgcgctgtt cgcggaccgc cgcctcaccc aggtccagca gttgcgcgag
    11701 gtaccggtcg gcaaatccgt ccttctttgc gcggacaaga agatcgtcgg gaagcggtct
    11761 gccgcggaag tgcaggatct cttcctcgag gtcgacgagt tctttcatct gcccgatgaa
    11821 ccagggcttg aggtgcgtct tcgccgaaag aagatccaca ctggcgccct tgcgcaatgc
    11881 ctcgtacatc aggaactgcc gctcgctcga cggctcggcg agcctctcga gcagttcttc
    11941 gagaggccgc ttgtgaaagt ccttcgcgaa gccgagcccg tggcgcccga tctcgaggga
    12001 gcggatcgac ttctggagcg cttccttata agtcttgccg atgctcatca cttcgccgac
    12061 ggcgcgcatc tgcgtgccca ggcgatcttc ggcgcctcgg aatttctcga aggcccagcg
    12121 ggcgaacttc accacgacgt agtccccgga gggggtgtac ttgtcgaggg tcccgctgcg
    12181 ccagtacgga atctcgtcga gcgtcagccc ggaggccagt ttcgcggaga ccagcgcgat
    12241 cgggaatccg gtagccttcg aagccagcgc cgacgagcgc gaagtcctcg ggttgatctc
    12301 gatcaccacc acccggccgg tcttcgggtc gtgagcaaac tgcacattcg tgccgccgat
    12361 gacctggatt gcctcgacga tccggtagga ctgctcctgg agtttccgtt gcaggtcctc
    12421 ggaaatggtc agcatcgggg cggtgcagaa cgagtcaccg gtgtggacgc ccattgcgtc
    12481 cacgttctcg atgaagcaga cggtaatcat ctggttcttc gagtcgcgca caacctcgag
    12541 ttccagttct tcccagccga gcacggactc ctcgaccagg atctggccga tcaggctggc
    12601 cgcgataccc cggctggcga cgatgcgcaa ttcctcgata ttgcggacgt gcccgccgcc
    12661 ggtgccgccg agcgtatagg ccagacggat gaccactgga tagcccagct tctccgcgat
    12721 cctctccgct tcctccacgg aatacgaggg ctcgctgcgc ggcatctcga ttcccagccg
    12781 cgccatcgtc tccttgaagg cgatccggtc ctcaccgcgc tcgatcgcgt ccacttgcac
    12841 cccgatcacc ttgacgtcgt atttctcgag tgtgccgttc ctcgccaatt cggcgcagag
    12901 gttcaggccg gactggccgc caaggtttgg gagcagtgcg tccggccgct cctcttcgat
    12961 gatctgcatc atgctcttga tgttgagcgg ctcgatgtag gtgacgtcgg ccatgcctgg
    13021 gtcggtcatg atcgtagccg ggttcgaatt caccagcacg atctcatagc cttgcgctcg
    13081 caaggccttg caggcctggg tgccggaata gtcgaactcg catgcctgcc cgatgacgat
    13141 cgggccggag ccgatgatca tgaccttatg aatgtcgttt cttcgtggca tcgttggagg
    13201 ttcttggaaa atggataaag agatccatat tagcgtggat cgaagcttcg gtgtcccttt
    13261 cagcggcggg cgtacaattg gacaagacgg agcctgctgt gcagcagagc ccgatactca
    13321 gaggaggtaa atatgacgaa gttccttcag gcgttgtgtc taagcttgtc cctattcgcc
    13381 tttgccgccg cggccgacca gatgaccggc tacatttccg actcgaagtg cggcgcggcg
    13441 cacacggacg gttctgaaaa atcggtcgct tgcgtgaagg cgtgcgtcaa ggggggcctc
    13501 gcaccggtgt tcgtaaccga ggacaacaag gtcctcaaga tcgacgacaa gtcgaaggtg
    13561 acggatttcc tcggcaagaa agtggaagtc actggttcgg tcgaggacga caccctcaag
    13621 gtcgacacta tcaaggccgc cgactgagtc cggccttcag agcgggagag gctattggag
    13681 cttgcctctc ccctccacct tccacacttc ctcaacgcgt tcgcctcgga tgccgtacat
    13741 cctctcgtgg aggttcacca ccacgcagac gtgattcgga atcatccgca cctggtcgcc
    13801 caccttgaac ttgcgcgagg actcgcggat atccacgtag ccgtgttcct cgttcatctt
    13861 gcggaatacg gccgcgggat cttccatcag ccggccgaag ctgacttcgt ccgagcccga
    13921 cagccggtcc gatgaaaacg tcttcgatcc gccgtcgatg atcatctgcc ccgggcgggc
    13981 agtggaaacg acagttgcga gcacggatgc cgcgcagtct tcccaggcgc aggcgcccgc
    14041 caggacggtg ttcttgtcat tgaagatata cgtgcccggg cggatctcgt tcattccacg
    14101 tacgagatgg gattggaaca gagtgggtgt ggagccgccg ctgacaactt gcggatcgaa
    14161 cccctcgtgg cggaggtcgg accggatcga ctcgacggtc gagccgagcg cctccatggc
    14221 ccgcaggccc tcgttgccgg tgatcctaag atgcccggga tagaaggcga cgccctccca
    14281 catcagtcca ggaagccggc tgatcgtctt cacgaaatcg acgagcggcg tacccgggtc
    14341 cacgccgacg cgtcccatac caacgtccgt ttccgccaga acgccgatgc tgacttctcc
    14401 ctcggaagcg gcttccgaca gcagggaggc acacagcatg ttgtcgaggg agacggtcac
    14461 ccgcgtttgc cgggctacct ccataagcct ttccagcttc ttccggccga acacggggta
    14521 ggccagcagg agatcgctcg gttgggcgcc cagcatcacc tccgcttcgc cgactttcgc
    14581 tatggtgagc ccggtcgcgc cgaggtcgag ctgccggcgg ccgagcgcgg gtgtcttgtg
    14641 tgttttggtg tgcgggcgaa gcctcaggtt gtgccgcatg gcgtaatcgg cgacacgctt
    14701 catgttccgc tccatgatgt caaggtcgat caggagcgcg ggggtttcaa ggtcttgaat
    14761 gtgcatggat cagttgcgga aacgcaggga gagattaccg gcaggaccct tgtagtacat
    14821 ctcgagctgc ttcgtcttgt gttttccctc catcaggaag tacagcagcc ccgaaaccgg
    14881 ttcggccgtt tctttgatgg gaagcacttt ggccttcaac gtcgccagga cgggatcgtc
    14941 cttctccttg tcggtggtgt ggacggatgt ctcggtctga acgctggtgt ttcccatagc
    15001 ggccccctcg ccaccgaggc ggcggggccg ccccatcgtc ccgccccaca ccggtccgct
    15061 ctcatccatc atcatgcctc ccgcaccgcc gcgcgcggaa accactagcg cgcccttgcc
    15121 ggcaatctgg ctcggtgcga acggtccggt ccgctgcccg tccttgtcgg agcgaaggat
    15181 gaagtcgtcg gggccgacgg cgaggggctt gtcacctttt ggcgtaacga caacctcgac
    15241 gatcacgatg tagccgccaa ggtcggtgcc gaccacagcc tggacggcct ccttgccgtt
    15301 gtgcaacgtg gccgccacct cgatgttatc gttcgatgcg ctgcccgtgg ggagtttgtc
    15361 agccgcggcg cacaagacca cgctcagcat cccgatacag atcgccctga aaatcattga
    15421 cgaccccccg agcgcattat acgaaattgg gggacccggt agtgagttga actgcctgca
    15481 gcccctccca gcctgagaat atcccctgtc tcccgccaca acctcgtcta ggagtcgtgc
    15541 gcgcggttgc ggagcgcgcc gccctgctgc tagattcaag aaaagggatc gcggcgggaa
    15601 ctccgcccgc atgcttgccg cgattcagag ccgcgaagac gagcttcact ggttggcact
    15661 gactttagtc ccgggcctcg gcgccagaaa agcgggacag ttaatcgaaa gattccgttc
    15721 gccgcaggcg gtatttcgtg cctcgaaaac ggaactggaa gcttgcggaa tcgggggtgc
    15781 ggtggcgcag agcatctcca gcggctgttc gttcgaggac gctgtcgccc agcaggagaa
    15841 aatgcttgag accgggacca gggtagttcc gatctccgat ccgcgatttc ccgagaaact
    15901 tcgcgagatc tacgatcctc cggtactgct gtttgtgctt gggagggtgg aactgctggg
    15961 ttcgctgatg cttggagtgg taggcacccg ccgtccgacg ccatatggtg tcgcggtggc
    16021 cgaacggctg gcggccgacc tggcgcgggc aggtttgacg attgtgagcg gaatggcgcg
    16081 tggaatcgac acatcggcgc acaagggagc tctggcggcc ggaggcgaca ccgtcgcggt
    16141 gctcggctgc ggcgtggacg tggtctatcc gtcggagaac cgaaagctgg cgggagactt
    16201 ggccgtgaag ggtctggttc tctccgaatt tccgatgggt gcacctgcgt atccgcagaa
    16261 cttccccgtc cgcaacagga tcatcagcgg aatgagcgtc ggggtaatgg tggtcgaagg
    16321 cgcccaatac agcgggtcgg cgatcaccgc aagactggcc atcgatcagg gcagggaagt
    16381 gttcgccgtg ccgggcaacg tgacatcgaa gatgagctgg gggcctaact tgttgattaa
    16441 acagggggca aagctcgtgc aggagtggaa cgacgtggtg gcggaactgc cggtcgaaga
    16501 acgccgccgc ttgattgcgg cgggccagag tcgcatactg tcggagggag gaggcagcgg
    16561 cgctgaaacc ggtgacggcc ggcaggcgtc actggacctc gggccttcca aggaactggg
    16621 caaaaggatt ctcgagttgc tcaaggtgga tagcgccaca cacttggatg atctgttgga
    16681 gcgtctggaa gatggcagct cttccgagac aatcgccgcg ctgttcgagc tggaaatgct
    16741 aggactagta cggcaactgc cgggaaagaa ctttgttaaa gtgtggtgac ggatcccccg
    16801 gcggaatttt ggattttgaa ttattgttaa caatcggaag caacggaatc atatgagcaa
    16861 gtctctcgtc atcgtcgaat cgccggcgaa ggcgaaaacc atcggcaagt acttgggcaa
    16921 gcagttcacg gtcagggcct cccttggcca cgtcaaggac ctgccaaaga aagatctggc
    16981 ggtggacatc gacaggaact tcgagcccac ctatgaaatc atcgaaggca agcggaagct
    17041 cattgccgag ttgaagcagg cggccaatgg cgtggacgcc gtctacctcg cggccgaccc
    17101 cgatcgcgaa ggagaggcca tctgctacca cttgcaggag gaactcgaac ccaagcggaa
    17161 cggaagcccg cggatctatc gcgtgatgtt caacgagatc accgcgaacg ccgtccgcaa
    17221 ggccttcgag aaacccggta tcgtcaaccg gaacctcgtc gacgcgcagc aggcccgccg
    17281 cgtgcttgac cggctcgtcg ggtacaagat ctctccgctg ctctgggaca aggtccggcg
    17341 tggactttct gccggccggg tgcagaccgt cgcgctacgg ctgatcgttg accgcgaacg
    17401 ggaaatccgc gctttccaga agcgggaata ctggaccatc gacgcgcatc tgctggccaa
    17461 gaagccaccg gttctagtgg cgcgtctcat gcgccgcaac gacgagcaga ccgagatacc
    17521 gaatcaggcg gcagccgaca acctcgtcgc cactctggat ggcgcccaat acgtcgtcaa
    17581 gtcggtcact acgcgcgaga agaagcgcaa tccggtgccg cccttcatca cgtcgacact
    17641 ccagcaggaa gccgcgcgca agctgcgctt cagcgcgaag cgcacgatga tgatcgccca
    17701 gcggttgtac gaaggcgtgg aaatcggcaa ggaaggctcg gttggcctca tcacgtacat
    17761 gaggaccgat tcgacgcgcg tttcgaccga agcccttgac gaggttaggc agttcgtacg
    17821 gcagcggttc ggcgagccct acctgccgtc agcaccgaat gtgtaccgga gcaagaagga
    17881 cgcgcaggac gcccacgagg cggtccgccc gacgtcagtg ctccgctcgc cggagacggt
    17941 cgaaaaacac ctcggcgaag acgagatgaa gctctaccgt ctgatctgga tgcgtttcat
    18001 cgcttcccag atgatgccgg cggtgttcga ccagacgacg gtcgatatca ccgcccgcgg
    18061 caaggacggc gctgagtacc tgttccggga gaccggttcg gtcatgaagt tcgatggctt
    18121 cctcgccgtc tatgaggagg gcaaggacca gaaggacgaa gacgacgagg agctaaagca
    18181 caagctgccg gttgtgcacg agggcgatgt gctcaagttc cgctcgatcc atcccgagca
    18241 gcatttcacc gagcctccgc cgcgctacac cgaagcaacg ctggtcaagg aactggaggc
    18301 tgatggcatc ggccgcccgt ccacctacgc ctcgatccta tcgacgattc aggaacggga
    18361 atacatcaag aaggaaggcg gccgtttcgc tcccaccgaa ctgggcatgg tggttaccga
    18421 ccttctgctc gagagcttcg aggacatctt cgacgtcacg tacacggcgc gcatggaaga
    18481 cgaactcgac gagatcgagg agggcaagat cgactggcgc gccgccatgg ccgagtttta
    18541 cgagaaattc gaggttgacc tgcgcaatgc cgagcggacg atgaccgaca tcaagcgcat
    18601 ggagaagccg acggacctcg tctgcgagaa atgcggcaag ccgatggtca tcaagtgggg
    18661 caggcacggg agcttcatcg cctgcacagg ctatcccgag tgcacgaaca ctcgtgagct
    18721 caacgtcgac cttccggacg ttgacaacgc cgacctcacc gagcagggcg acgaagaata
    18781 ctgcgccaac tgcggccggc cgatggtgct caagaaaggc cgtttcggcc agttctacgc
    18841 atgcagcggc tatcccgact gcaagaccac gaagcccatc ggagggcagc agaagaaggc
    18901 cgacgtgccg cttgaagaga agtgcccgaa gtgtggcagc aatctcgcgc tgaaacacgg
    18961 gcgcttcggc gagttcaccg cgtgcagcaa ctatccgaag tgcaagtacg tgaaacagaa
    19021 gacgatcggg gtcccttgcc ccaactgcag cgagggtgag ctgatcgagc gccggtccaa
    19081 gcgcggcaag acgttctacg gctgcaaccg ctatcccgag tgcgacttcg ttgcatgggc
    19141 gaagccggtc gccgagaagt gccccgaatg cggcagtccc tacctggtgg agaagtggct
    19201 caaggcaggc ccgcagctcc agtgcccaaa cgccgagtgc aagtataagc gcgccctcgc
    19261 cgtccccgag cccgtagcat cctgaagcgc caggcgtcat agcaacccgg cgcttcaccg
    19321 accgcaccgt acgagcctac ccaatctctg ggttccggat cccgccgacg tctattcggc
    19381 ccttttcaaa gttccgacac gtatcgagac ctcgcagcca ccggcaaatt tggtaaaatg
    19441 caaagaaatt gtctgggcaa tgactctgaa aacgaagagg gatattgatg ccaaagaaac
    19501 gcacgtctga aaccgcaccc cgttcctcca gtggtgatcc ggctccggcc aagaccactg
    19561 cccggaagcc gagcgttgcc aagtcccagg gcaacggcgc cgttcagccg gttccccggt
    19621 tagtccggag gaaggcgccc ttggcgcccg tcgggcaaca gggatcgccg gctgctcatt
    19681 ccgttcctcg ggacgaggtt gcccggctgg cctattccta ctgggaatcg cggggcggcc
    19741 gcggcgggtc accggaggaa gactggtttc gggccgaaca agaggtcctc agccgcatcg
    19801 agcggccgtc cgctcagtag acaacaatct tagcggtact tccggagcac ggcgagcaca
    19861 cgcccctgaa tccggaggtc ggatagcggc acgaatatcg gggccatccc ggcattggcc
    19921 ggctgtaacc gtgctacatc tcccggctcc cgatagaatc gcttcagcgt ggtctcggac
    19981 ccacggacca gtgcgacgac gatctccccg tctttgacct cggccgtgcg ctcgaccaga
    20041 acgaggtcac cgtcgcaaat gtggtcctcg atcatcgagt cgccgcgcac ttcgagggcg
    20101 taactttcac cgctgccgag aaaatcgctg aaattcaacg tctcacggcc ggccaccgcc
    20161 tcgactggag cgcccgccgc gatgcggccc accagcggaa tccccgcggc tggactggtc
    20221 tcctcgcgat agcgccgctg ttcctggaag aactttggtg tcacctcaag cgaccggctc
    20281 tggttgtggc ctcgccggag gtaatttttg gcctccaggg tccggatatg tttgtgaact
    20341 gtagccagcg aagcgagccc gagcccggat gcgacttcct cgaagctggg gctgtagccg
    20401 ttctcgtcga tgaagttgcc aatgaaatcc agcacctctt tctgccggcg cgtcaggggc
    20461 atcggtcctc cttgtctcct attgttggcg aataaaaaga gaaagtcaac tgcgatttca
    20521 aagggggccg ggtactggta cgttcacgga cgctttgtac ctacccctcc ccgcaattgc
    20581 gtggtttact gttgatagca ttgctgctga ccagggttat catccggtcg gtagcgaggg
    20641 cagttggttt tgacctgaat ggacggagag cgttcgctat gaccttcatc tgggggttgt
    20701 tcgagaaacg aattgcggat atgcggatgg atgagaaggt gtttcttctc ctgatcatcc
    20761 tcgcctgttt ctgcctggtt acctacgcga tgtcattgat cgtgcaaatc gttcgtaaga
    20821 tggagcagca ggagtcccca gagcaacagg cgccagcgtc cctccgggct ggattgctga
    20881 attcggaagc gttgcgccgt tacgacggcc cgcgatacgg ccatacagac tgacggaact
    20941 cggaaggttc gttttctcac cttctttctt caccacagag cgcacaaaca aaacagaggg
    21001 aagatgtttc gatgaccctc cgctatctgt ggtgatccgc atagtttcct cttcccacga
    21061 cagagaacgc agagggtaca gaggaaagat gtttcaacat tgctctctgc gccctccctg
    21121 ctctctgtgg tggtgcgttt ggggcgattc cattcttgcg tctgtgctga taagctctat
    21181 gcacgtaagg agtggagact gatgccattt tcaaagcaat ctcgactgag tcgtaggaat
    21241 ctgatcgccg gtgcggcggc gttcgccggt tttttcaaaa tgaccgaaag cgacgcgcgg
    21301 gcgcaggcga gagcacggca gggattcaat cggaactcgg cgccaagcca gctcaaaatc
    21361 accgatatgc ggtcggttct ggtggcgtcg aactacgact acccgatcat ccgcattgat
    21421 accaatcagg gcgtctatgg ccttggcgaa gtacgcgatg ccggtaggga aggcaccgcg
    21481 ttggtgttga agccgcatat catcgggcga aatccactgt cgatcgagcc gattctggac
    21541 agactgagaa tcttctgcaa tcaccagcgg atgggcggcg gttacagcgc cgtggacatg
    21601 gcattgcacg acatcgcggg caaggtgtac ggcgtgcctg cgtggcggtt ggtcggatcg
    21661 aagtaccgtg acaaaattcg catctactgc gacacagatc agagcaaaga cccgaaggtc
    21721 ttcgcggaac ggttgaggaa gcgggcccag cagggattca cgtttttcaa aatggatctc
    21781 ggaaccagcc tcgtggagga caagcccggc gccgtcgaca gccgccgtgt agctaccgaa
    21841 aaaggtctgc aactgctcgg cgagtacgtc caggcggtac gggaccagat cgggtgggaa
    21901 gcgcctctcg ccaccgacca cttcggtgcg ctgacagtga acgatagcat ccgttacgcc
    21961 cgggcgttcg agagatataa cctggcctgg gcggaggaca tgatccagat cggccacctt
    22021 gggcccggag gcgacgctcc gaaagactgg cgcggctaca aggaactcaa ggaggccacg
    22081 accacaccga ttgcgaccgg tgagagcctg ttcggcctgg aagaaggctt caaggatctg
    22141 atcgacaatc gagccatcga catcattcat cccgatccgc tgacatccgg cgccattcgc
    22201 gagacgaaac gaatcgccga ttatgcgtcg atgcacggca tcgagacagc catccacttc
    22261 gcgggttcac cggtcggctg cctggcgagt gtacacatgg ccgcgaccct gaaagacatg
    22321 ctcgccatgg agaaccatgc cgtcgatatt ccctggtggg gagacctggt gaccggaccc
    22381 tcgaaaccga tcgtcgaccg cggcttcatt gcggtccccg atacgcccgg acttggcatc
    22441 gagttgaacg aagacgtggt gaaggagcac atccggaagg gtgggtattt cgctccgacg
    22501 ccgcagtacg acgactacat cctcgatcac ttccggcgcg ggggaccgta cccacacatc
    22561 gacgaaaacg gcaacccggt ggtgagccaa taacgttccg ccgcgggcga agcttaattt
    22621 cgcccgcaca tcccgggcgt ggggcacacg ccgccggaag gacacatcgg cacgtcggaa
    22681 cgggttcccc cgacgcgcgg ggcgaatgtg gacagcttct gcgtgaggtg catctcaccg
    22741 cacgaggggc aagccacgct ggtggagtcg gcgacgcggt tcacgcgttt ttcgaattcg
    22801 gttccgcaat cgtcgcaacg gtattcatag atcggcattc acgtattccc cttaccgggc
    22861 gatcacacta cagattttat caatgatggc gcggatttct cgcgacggag cgttgtagtt
    22921 gttgacgaga atcgaaaacg cgagcatccc gttcgactgg ctgtccacat agcctgagag
    22981 tgcactcacg tgaccgagcg tgcctgtttt ggctcggacg cggctgccgt cccgcattcc
    23041 tttgaaccgg tcgctgagcg ttccatcttc ccctcccaca ggcaacaggc tgttccagac
    23101 ctccgcgcga gccgaccggc tcatcgcgag cagcagcttt gtcaccgctt cgggcgtcac
    23161 cagattgagc cgtgacagcc ccgaaccatc ctccatggtg tattcagagc cggcaatatc
    23221 ggcgtcttga aggaaccccc gcatttccac gaggcccgcc tgccggctgc ccaggtttct
    23281 tcgtgcccgc cccacctcac gaagcgcaat ctccgcatgc aggttctggc tgaccttgtc
    23341 aatcactcgc agaatctcag ccaaagggag cgattcgcgc cgtgccagtt cgacaccagg
    23401 agacggcgcc gacgcctcgc caccgccctt catgtcgagg acatcgctag ccgaacggtg
    23461 ccgggcgacc gcttttccca agaccgccac gccgcggcga atcaatgctt cgcgcagagc
    23521 ggtagccgcg tacagcgccg ggtcgtccgc ggcgaccaga acgctgactc cgcggtcttt
    23581 ggcaggcatg gcgccccaca cacgtaattg ccgggacccg ggtgcccggt cgatccggac
    23641 attccgctcg ccggcggcgg ccgccgtcgc aacgcggttg tcgatcgcga agtaccctgt
    23701 atccggggtc aaccggagtt gcgcgggatc gcccatttcc gggcctggtc tgacgcgcaa
    23761 cgtcatcgca ttgtcgttga acgtcagcgc gctgaccggc gcgccgtact cccacaggct
    23821 gtcgtcctgg gcccagccct ccggatacgg ctcccagaca taagcagtgt cgtctccggc
    23881 gatgtcaccc gcaacgagtc tgactcctct cagtccgacc tgttccgcca gttcctcgat
    23941 cgctctcatc gggtcgccgg cccgagcgtc cttacggtat gggtactcgc ggcccgacat
    24001 ggtcggatca cc
//
LOCUS       DATIFQ010000279        11380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2196778, whole genome shotgun sequence.
ACCESSION   DATIFQ010000279 DATIFQ010000000
VERSION     DATIFQ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11380
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196778"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..2016
                     /gene="hscA"
                     /locus_tag="VLE22_13165"
     CDS             163..2016
                     /gene="hscA"
                     /locus_tag="VLE22_13165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HSB15403.1"
                     /translation="MSDTFQIDFSASERPLVVGIDLGTTNSLVASMDLTGPRVIPGAD
                     GKNLVPSVVSYSRAGEIVAGSLAREMLITEPERTVYSVKRLMGRGAADVQEELKLFPF
                     RIAEGSESVIRLELGERRFTPPEISAFILRQLKQNAEADLGETVTKAVITVPAYFNDA
                     QRQATKDAGRIAGLEVLRLVNEPTAAALAYGLDKRKEGIVAVYDLGGGTFDISILRLH
                     DGIFEVLATNGDTHLGGDDIDNLLLQIALEDVRTEWGEEISNDGTAVQTLRRAVIEAK
                     ERLSYAPFTSIDSEYRGRKYQRELTRDLFEQLITPIVNRTLGPCRNCIQDARVSVEQI
                     DEVVLVGGSTRIPLVRKAVETLFKTEPHKELNPEEVVALGAAVQAGILAGSVENQLLL
                     DVTPLSLGIEMMGGVVTKLIHRNSTIPASATEVFTTAVDGQQNVLIHVVQGEREMARD
                     CRSLARFDLKGVEPLPAGMTRIEVRFLIDANGILSVTARDIRTGKEQTVEVKPSYGLT
                     DCQVEAMIRESEEMAEEDFAERQLREARLEADTILAALEKAKQNEAWFELTGEEREQA
                     DLAMNQLLLAYHSDDHLLIRAQVEHLNQATMKLAEMMMNTAVRGALKGTKI"
     gene            2016..2228
                     /gene="iscX"
                     /locus_tag="VLE22_13170"
     CDS             2016..2228
                     /gene="iscX"
                     /locus_tag="VLE22_13170"
                     /inference="COORDINATES: protein motif:HMM:NF016284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HSB15404.1"
                     /translation="MQKLSWNDAENIAIVLFEKFPNVDPLTVRFTDLHRWVVELTEFS
                     DDPKASSEGKLEAIQMAWHEEWSDNQ"
     gene            complement(2244..3245)
                     /locus_tag="VLE22_13175"
     CDS             complement(2244..3245)
                     /locus_tag="VLE22_13175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HSB15405.1"
                     /translation="MIDFFPMSHYWWLYLAFTGFVLLMLALDLGVFHRKAHVVSFREA
                     AAWSSVWVALALTFNYAIYQYALWKFPLDPRLSATPGFDSAAAARQVGLEFLAGYLVE
                     KSLSVDNIFVFVLVFSYFAIPAQYQHRVLFFGIVGALVFRAIFIAIGSVLMQYHWVVI
                     LFGVFLVLTGLKMLFAPEKGLEPDRNPLIRLFRKFVPVTDELHGQKFFVRLKGVLYAT
                     PLLIALLFVEVTDIIFAVDSVPAIFALTREPLIVFTSNVFAILGLRALYFMLADAIGK
                     FHLLKYGLALVLIFVGLKMAWLNGMFGGKFPIGWSLGIIGGLIAVSVTLSWLIPKRA"
     gene            3516..3719
                     /locus_tag="VLE22_13180"
     CDS             3516..3719
                     /locus_tag="VLE22_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15406.1"
                     /translation="MNRSKEQLELAEAVLELIERRRATTGNPELGSAIERAILEQEIR
                     GLEHEIFENPGALETMLVPIRRR"
     gene            3818..4012
                     /gene="rpmI"
                     /locus_tag="VLE22_13185"
     CDS             3818..4012
                     /gene="rpmI"
                     /locus_tag="VLE22_13185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HSB15407.1"
                     /translation="MPKLKTHKGASKRFKKTGTGKIARRHAFSRHILTSKTRSRKRRL
                     GQSTIVDEADAAKLSRMLPN"
     gene            4093..4458
                     /gene="rplT"
                     /locus_tag="VLE22_13190"
     CDS             4093..4458
                     /gene="rplT"
                     /locus_tag="VLE22_13190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSB15408.1"
                     /translation="MPRVKRGTTRRASRKKTLDRASGFFLTKSKLHRSAQEAVEKAQR
                     YAYVGRKRKKRDFRGLWITRIGAATRQLGLSYSKFMSGLKKAGVDLNRKVLAHLALTD
                     ETAFRLLVDKAKAALPKAD"
     gene            4458..4703
                     /locus_tag="VLE22_13195"
     CDS             4458..4703
                     /locus_tag="VLE22_13195"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HSB15409.1"
                     /translation="MDLLRDEQTDALASLEERILKAADLVRRLRQERDVAVQGLEKAV
                     AERDAVVQELEALRAERKQVRTRIEKLLGQMDLLSAG"
     gene            4746..5057
                     /locus_tag="VLE22_13200"
     CDS             4746..5057
                     /locus_tag="VLE22_13200"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HSB15410.1"
                     /translation="MDSEQAEKKSVRVTIFHQSYTLRVPGDPQEVQELARTVDDLMDS
                     ISSRTGSSDTTRVAVLACLHMADRLRSLEQDLNTLRERIGKTSEQFTLLLDQALEGHE
                     E"
     gene            complement(5074..5934)
                     /locus_tag="VLE22_13205"
     CDS             complement(5074..5934)
                     /locus_tag="VLE22_13205"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HSB15411.1"
                     /translation="MTSALEESYAWCTRLTRRRARNFYYGFALLPKPKREAICAIYAF
                     MRYCDDLSDEPGMGGGRGREMMSRWRAQLEEALAGRPGDHALWPAFLDVVERYRIPRR
                     YFFEMIEGVSFDLEPHRFTTFDDLYQYCYRVASVAGLTAVHIFGFDSADALPLAEKCG
                     IAFQLTNILRDVREDSARGRIYFPLDDMNRHGVTSDEVLRAARTDGLVQLMRYEAGRA
                     RAYYTEATPLVKMVHPESRASLWALIEIYRRVLDRIEDSNYDVFTGRIRLPVPEKCWI
                     VARSLLRRHV"
     gene            complement(5931..6854)
                     /gene="hpnC"
                     /locus_tag="VLE22_13210"
     CDS             complement(5931..6854)
                     /gene="hpnC"
                     /locus_tag="VLE22_13210"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HSB15412.1"
                     /translation="MSSAFLLPRDFVRSTEALARTSNSSEAADYTRWLATHHYENFHV
                     VSFMLPKRLHQDFYNVYAFCRWADDLGDEIGDPKESLRLLEWWREQLEAMYAGCASHP
                     VYVALRETVERHSLPAEPFRDLIRAFVQDQAVTRYQTYTELFDYCRLSANPVGRLVLY
                     LCGYKDEERRRLSDATCTALQLANHWQDISVDLEKDRVYLPVEALDRHGSSLADVLGR
                     RATPGFRAALREAADVARRLFHQGLPLARMVGGRLAVDLDLFSRGGLLILDKIESQDY
                     DIFSRRPHVTKTERIRLLISTLARVAISRAA"
     gene            complement(6858..7898)
                     /locus_tag="VLE22_13215"
     CDS             complement(6858..7898)
                     /locus_tag="VLE22_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSB15413.1"
                     /translation="MKTQMMAAVLYGKEELRVEPVAVPDLAEGDLLVRVKAALTCGTD
                     VKVFLRGYHARMIVPPAVFGHELAGDIVAAGSGVRKFRVGQRVVAANSAPCYQCYYCR
                     RGLENLCEDLLFNNGAYAEYIRIPARIVERNTYVIPDHIAYQDAALVEPLSCVLRGLE
                     ETGVRAEDTVAVIGLGPIGLMFVRMAKVWGARVIAVGRRKTQLDRAAKLGAEEVVTAG
                     DNDDPVSAVKSLTGGRGADIAIEAVGSPSAWEQAVQLVRRGGTVNFFGGCPNQSRVCL
                     DTSLLHYSEITCKASFHHTPQHIRMALEVIGDGHVSAMDFVKGEEPLANLLEVMRYLM
                     SHNGHLKTAIIP"
     gene            complement(7895..8995)
                     /locus_tag="VLE22_13220"
     CDS             complement(7895..8995)
                     /locus_tag="VLE22_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSB15414.1"
                     /translation="MQTVDSDLTTPPETMRAAVYKGNGTVSVERVTTPVIGPGELLVR
                     VEACGVCHTDLKKIEYDLLAPPRIFGHETAGVVAAVGEGVREYTPGDRVIVFHHIPCT
                     ECFYCRKKLYAQCPVYKKVGVTAGFEPAGGGFSQYVRVMDWIVRRGVERIPEGVPFER
                     AAFVEPVNTCLKAIQSLNPQPGELVAILGQGPIGLMFTMLVRRTGAAIVSTDTILRRR
                     ELSVRFGSQSVWDPRAADVVREVAALTGGRGADAVVVAASAPGITEQAMRSSRPGARI
                     LLFAQTSHQERVEFSGADICVGERTVLGSYSASIDLQAESARLVFSGELPVEDLISHC
                     LPLDEINYGIRLALHPGEQSLKIMVSPQRRTE"
     gene            complement(8976..9680)
                     /locus_tag="VLE22_13225"
     CDS             complement(8976..9680)
                     /locus_tag="VLE22_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15415.1"
                     /translation="METILVVAAEVRELVGLVRRCGKMENPGWPLDFVRLAELNGYRL
                     VLAANGTGPERAAEAFDVAARNGKLDRVVSTGSCGALDPSLEAGDVFVASRVEDAGGG
                     GSYRTEVPGSPQAKAIGVLFSVDRVVRTAAEKRALRACGASVVEMESAGLAARAAGAS
                     LPFYCVRAVLDTAMEEFDTDFNRVRDSSGRLRTARIVRAALARPFRRVPELYRLHRRS
                     KLVARSLGDFLADCRF"
     gene            complement(9682..10656)
                     /gene="hpnA"
                     /locus_tag="VLE22_13230"
     CDS             complement(9682..10656)
                     /gene="hpnA"
                     /locus_tag="VLE22_13230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HSB15416.1"
                     /translation="MRPTLITGATGFVGWHVARNLIERGHRIRVLVRPESRLRELEAE
                     TATGDLRDPESLQRAACGCGVVFHVAADYRLWTKHPQEMHRSNVDGTRNMLEAARRAG
                     VERFVYTSTVGCIGIPATCLGDEDTPVGLHDMVGAYKRTKFLAEQVALEYSRSGFPVV
                     IVNPTAPVGDHDFKPTPTGKIVVDFLRGAMPAFVDTGLNLVDVRDTAEGHRLACERGR
                     VGERYVLGCENLTLSEILRKLGVASGLKAPSTRIPYGVAWTAGVLSTGLARLTGGDPR
                     IPFDAVRMARKKMFVRHDKAARELGFTPQAVDGALRRAVEWFRQNGYC"
     gene            complement(10666..11139)
                     /locus_tag="VLE22_13235"
     CDS             complement(10666..11139)
                     /locus_tag="VLE22_13235"
                     /inference="COORDINATES: protein motif:HMM:NF023372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3463 domain-containing protein"
                     /protein_id="HSB15417.1"
                     /translation="MKEIEALFEYLEPFGVDGHMLAPGYGYSAVNDREIFMTREDVHE
                     KFKDIDRLARRFRLNSTPVYVDFLKGTRDLPCTAWGNPTYNVSGWKGPCYLITDGHYE
                     TFEEFMVRTPWEDYGHGNDPRCEHCMMHCGYEPSAALGINGKASDSLRLLSWALR"
BASE COUNT         2386 a         3569 c         3455 g         1970 t
ORIGIN      
        1 tctcttcggg aggacgtcga cggcgacctt gaggcgcggt tccgtgagta cgatgcgagt
       61 cggcagcggg aaatcctggg aagtatccgg gcgctgctga accggcgccg atatatcgag
      121 aacctgcttc acgaggtgga gcgcgagttg atgaactagg ccatgtccga cacgtttcag
      181 attgatttca gcgcgagtga gcgtccgctg gtcgtgggaa tcgacctcgg cacgaccaac
      241 agccttgtgg cgtccatgga cctgactggt ccccgtgtca ttcccggagc ggacggaaag
      301 aacctcgtgc caagcgttgt cagctacagc cgggccgggg agatcgtggc cggcagtctt
      361 gcgcgggaga tgctgatcac cgaaccggag cggactgtct attccgtgaa gcgcctcatg
      421 ggccgcggcg ccgcagacgt tcaggaggaa ctgaagctct ttccgtttcg cattgcggag
      481 ggcagtgagt cggtgatccg gctggaactc ggcgagcgga ggttcacgcc gccggagata
      541 tctgcattca tcctccgcca actcaaacag aatgccgagg cggacctcgg tgagacggtg
      601 accaaagctg tgatcacggt accggcatat ttcaatgacg cccagcggca ggccactaag
      661 gacgccggac gaatcgccgg gctggaggtg ttgaggctcg tgaatgagcc aacggcggcc
      721 gcgctcgcct acggactgga caagcggaag gaaggaatcg tcgctgtcta cgacctcggc
      781 ggcgggacat tcgacatttc gatcctccgg cttcacgacg gcatcttcga ggtactcgca
      841 accaacggag acactcacct aggcggggac gatatcgaca atctcctgct tcagatcgcg
      901 ctggaagatg tccggacgga atggggagag gaaatctcca acgacggcac ggcggtgcag
      961 acgctgcggc gagcggtgat cgaagccaag gagcggcttt cctacgcgcc gtttaccagc
     1021 atcgattctg aataccgcgg ccggaagtac cagcgggagc tgacccggga cttgttcgag
     1081 caactgatca cgccgatcgt aaaccggacg ctcgggccat gccggaactg catccaggac
     1141 gcgcgcgtga gtgtggaaca gatcgacgag gtcgtgctgg tcggcggatc gacaaggatt
     1201 ccactggtgc gaaaagcggt ggagacgttg ttcaagaccg aaccgcacaa ggaactgaat
     1261 ccggaggagg tagttgcact tggcgcggcc gtccaggcgg gaattctggc gggcagtgtc
     1321 gaaaaccagc ttctcctgga cgtgacgccg ctctcgctcg gcatcgagat gatgggcggc
     1381 gtggtcacga agctgatcca tcgaaactcg acgattccgg cgagcgccac cgaggtgttc
     1441 accaccgcgg tggacggaca acagaacgtg ttaattcacg tcgtgcaggg agagcgggag
     1501 atggcccgcg actgccggag cctggcgcgc ttcgacttga agggtgtcga gccgttgccg
     1561 gcggggatga cgcgtatcga ggtgcggttc ctgatcgacg ccaacgggat actgagcgtg
     1621 accgcacgcg atatccgcac ggggaaagag cagaccgtcg aagtgaagcc gtcttacggg
     1681 ctgaccgatt gtcaggtgga ggcgatgatc cgggagtccg aggagatggc cgaggaagac
     1741 ttcgccgaga ggcagctaag ggaggcgcgt ctcgaagccg acacaattct ggcggcgctg
     1801 gagaaggcca agcagaacga ggcgtggttt gagctgaccg gcgaggagcg cgagcaggcg
     1861 gacctggcga tgaaccaact gctgcttgcc taccactcgg acgaccacct gctgatccgg
     1921 gcgcaggtgg aacatctcaa ccaggcgacg atgaaactgg cggagatgat gatgaataca
     1981 gcggtgcgtg gcgcgctgaa gggaaccaag atctgatgca gaaactgtcc tggaacgacg
     2041 cggagaatat tgccattgtg ttgttcgaga agtttccaaa cgtcgatccg ctgacggtgc
     2101 gcttcaccga tcttcaccgc tgggtggttg agctcacgga gttcagcgac gatcccaagg
     2161 cctcctcgga gggcaagctg gaggcgatcc agatggcttg gcacgaagag tggtcggaca
     2221 accaataggg ccggcagccg caattacgca cgctttggaa tcagccagga gagcgtcacc
     2281 gaaacggcga tcagcccgcc gatgattccg agcgaccagc cgatcgggaa tttccctcca
     2341 aacattccgt tgagccacgc catcttgagg cctacgaaaa tcaggaccag tgcaagaccg
     2401 tacttcaaca ggtggaactt accgatcgcg tccgcaagca tgaagtagag agcgcgcaga
     2461 ccaaggatcg cgaagacatt cgaggtaaac acgatgagcg gctcgcgggt gagcgcgaag
     2521 atggctggca ctgagtcgac cgcgaagatg atgtccgtca cctcgacaaa cagcagcgcg
     2581 atgagaagag gggtcgcata gagcacgcct ttcaaccgca caaagaactt ctgtccgtgc
     2641 agttcatcgg taacgggcac gaatttcctg aacagccgaa taagcgggtt ccggtcaggc
     2701 tccaatccct tctcaggcgc gaacagcatc ttcagccccg tgagcaccag aaaaacacca
     2761 aacaggatca cgacccagtg gtactgcata agcaccgacc cgatcgcgat aaagattgcc
     2821 cgaaatacca gagcaccgac gatcccgaag aacagcaccc ggtgctggta ctgcgctgga
     2881 atggcgaagt aactgaatac gagcacgaag acgaaaatgt tgtccaccga gagggacttc
     2941 tcgacgagat aacccgctag aaactccagc cctacctgtc gggcagccgc cgcggaatca
     3001 aagccgggtg tcgccgacag ccgtggatcg agcgggaatt tccagagcgc gtactggtag
     3061 attgcatagt tgaacgtaag tgccagcgcc acccagaccg agctccaggc agccgcctcg
     3121 cggaacgaga cgacatgggc ctttcggtgg aacacgccca ggtcgagggc cagcatcagc
     3181 agcacgaacc ccgtgaatgc gaggtagagc caccagtagt gagacatcgg gaagaaatcg
     3241 atcatttatt tcaggttccg ggagctcacg gagacggctt aaccgcaggt cgggacgcct
     3301 gcttcgcacc gccgcaagtc gctgattcag aacgcagaac tctcctccgc gttccagagt
     3361 tgaccctcgc tcttaaccat cagatgcggc agacaccgaa ccgtcgcatt gtgatcaccg
     3421 ccggtcgtcg tccaggcgcc ggccattccg acgatacgaa aaaggtttgg aactttagca
     3481 cgaggcccgc gtctgaatgg gtaggaggtg gggccatgaa ccgcagcaaa gagcagttgg
     3541 agctggctga ggcggtgctc gagctgatcg agcggcgccg cgcgacgacg gggaatcccg
     3601 aactcggctc cgccatcgag cgcgccattc tggagcagga gattcgcggt ctggagcacg
     3661 aaatcttcga gaacccgggt gcactggaga caatgctcgt cccgatccgg cgccgctgac
     3721 ccgcgcaggc gcgggcaacc ccgcccgtga tatactcaag acgtttgttg tgagcccttc
     3781 gcccgctctg gcgggcggtc caaaggaaat tttgtcaatg ccgaagctga agacacacaa
     3841 aggtgcgtca aaacgtttca agaagacggg cacgggcaaa atcgcccgcc gtcatgcgtt
     3901 ttcacgccat attctgacgt cgaagacgcg ctcgcggaag cggcgcctcg gccagagcac
     3961 gatcgtcgac gaagccgacg ctgccaagct cagccgcatg cttcccaact gagatctatc
     4021 cggcgagcgc ggccggagcg cacggcacgt gcccgtaccg cctcgccatg agctatccga
     4081 aaggagaaaa acgtgcctag agtcaaacga ggtaccaccc gccgggcgtc gcgaaagaag
     4141 accctcgatc gcgcttccgg tttctttctc accaagagca agctgcaccg ttccgcccag
     4201 gaggccgtcg agaaagcgca acgctacgcc tacgtcggcc gcaagcggaa gaaacgcgat
     4261 ttccgcggtc tttggatcac ccggatcggc gcggccacgc gccagcttgg gctttcctat
     4321 agtaaattca tgtccgggct gaagaaagcc ggcgtcgatc tcaaccgtaa agtgctggct
     4381 caccttgccc tgaccgatga aacggcgttc cggctgcttg tagacaaggc aaaggcggcc
     4441 ctgccgaagg cggactgatg gacctgctgc gcgatgagca gaccgacgct ctggccagcc
     4501 tggaagagcg catcctcaag gcggcagatc tcgtaagacg gctccgtcag gagcgtgacg
     4561 tggcggtcca ggggcttgag aaggctgttg ccgagcggga tgccgttgtg caagaacttg
     4621 aggccctccg cgccgagcga aagcaggtcc gcacccgcat cgagaaactt ctcgggcaaa
     4681 tggatctgct cagcgccggc taaccgagcg gcggcttcgg tctccgctct ttctccggag
     4741 aagcgatgga ctccgaacag gccgaaaaga aatccgtccg agtcactatc ttccaccagt
     4801 cctacacgct tcgtgtgccg ggggaccccc aggaagtgca ggaactggcg cgcacagtcg
     4861 atgatctgat ggactcgatc tcctcgcgga ccggaagttc cgatactacc cgggttgccg
     4921 tgctggcctg cctccacatg gctgaccgcc tgcgctccct ggaacaggat ctcaacacgc
     4981 ttcgcgagcg cattgggaag acgagcgagc agttcacact tctcctcgac caagccctcg
     5041 aagggcatga ggagtaaccg gggggactgc cggctatacg tggcgccgaa gaagggaccg
     5101 ggcgacgatc cagcatttct ctggcaccgg aagccggatg cggccggtga agacgtcgta
     5161 attcgaatct tcgatccggt cgaggacgcg gcggtagatc tcgatcaacg cccaaagtga
     5221 cgcacggctc tcagggtgca ccatcttcac gagcggggtt gcctcggtgt aatacgcgcg
     5281 cgcccgtccg gcttcgtaac gcatgagctg caccaagccg tcggtccttg ctgcccgcag
     5341 cacttcgtcg gaagtcacgc cgtgccggtt catatcgtcc agggggaagt agattcgccc
     5401 ccgtgccgaa tcctcccgca cgtcgcgcag gatgttagtc aactgaaacg caatcccgca
     5461 cttctcagcc agtgggaggg catcggccga gtcgaaaccg aagatgtgca ccgctgtcag
     5521 gccggccacc gatgcaaccc ggtagcaata ctggtaaaga tcatcgaagg ttgtgaaacg
     5581 gtgaggctcg agatcgaacg acacgccttc gatcatctcg aagaaatacc ggcggggtat
     5641 ccgataacgc tccaccacgt ccagaaaggc cggccacaga gcatgatctc ccggccgtcc
     5701 ggccagggct tcttccagtt gcgcacgcca gcgggacatc atctcacgcc cgcgccctcc
     5761 tcccatcccg ggctcgtcgc tcaaatcgtc gcaatagcgc atgaatgcgt agattgcaca
     5821 gatcgcctca cgcttcggct tcggaagaag ggcgaaaccg tagtaaaagt tgcgagcccg
     5881 gcggcgggtc agccgggtgc accatgcgta ggattcctcc agtgccgacg tcatgcggcc
     5941 ctcgatattg ctacccgggc gagggtcgag ataagaagcc tgattcgctc tgtcttggta
     6001 acatgcgggc gccgggagaa tatgtcgtag tcctggcttt cgatcttgtc cagaatgaga
     6061 aggccccctc ggctgaacag atccagatcg acggcaagcc tgccgccgac catccgcgca
     6121 agcggcagac cctggtgaaa caatctccga gccacatcgg ccgcttcgcg caaagcggct
     6181 cgaaatccgg gcgtcgcccg ccgaccgaga acgtcggcga gcgaactgcc gtgccggtcg
     6241 agggcttcta caggcaggta cacgcggtcc ttttcaaggt cgacggagat gtcctgccag
     6301 tggttggcta gctgaagcgc cgtacaggtg gcgtccgaga gccgccgacg ctcttcatcc
     6361 ttgtatccgc agagatacag aacgagccgg ccgaccgggt tggcggagag ccggcaatag
     6421 tcaaacaatt ccgtgtaggt ttggtagcga gtcacggcct ggtcctggac gaatgcccgg
     6481 atcagatcgc gaaagggttc ggccgggaga ctgtggcgtt cgaccgtttc gcgcaacgct
     6541 acgtaaaccg gatgcgaagc acacccggcg tacatcgcct caagttgctc cctccaccat
     6601 tcgagcagcc ggaggctttc tttcggatcg ccgatctcgt caccgaggtc gtccgcccac
     6661 cgacagaagg cgtaaacgtt atagaagtcc tggtgcaggc gcttcggcag cataaacgag
     6721 acaacatgga agttttcgta gtgatgagtc gcaagccagc gggtgtagtc cgccgcctcc
     6781 gaggagttag acgtacgggc cagcgcttcc gttgaccgga caaaatccct cgggagaagg
     6841 aacgcgctgg acaaggctta gggaataatg gcggtcttca ggtggccgtt gtggctcatg
     6901 aggtagcgca tcacttcgag gaggttcgcg aggggttctt ctcccttgac gaagtccatc
     6961 gcgctcacat ggccgtcacc gatcacctcg agggccatcc ggatatgctg cggcgtgtga
     7021 tggaagctcg ccttacaggt gatctcggag tagtggagaa gcgaggtgtc cagacatacg
     7081 cggctctggt tggggcagcc accgaagaaa tttaccgttc cgccacgccg gacgagttgt
     7141 accgcttgct cccacgcgga gggggatcca acggcctcga tggcaatatc ggcgccgcgg
     7201 ccgccggtga ggctcttcac cgcactgacg ggatcgtcgt tgtcaccggc agtgaccacc
     7261 tcctcggcgc cgagcttcgc ggcacggtca agctgggttt ttcgccggcc taccgctatc
     7321 acccgggcgc cccagacctt cgccatacgg acgaacatca gtccgatcgg cccgagcccg
     7381 atgacagcca cggtgtcctc cgcacgcacc cctgtctcct caagaccgcg cagcacgcac
     7441 gacagcggct ctacaagggc tgcatcctga taggcgatgt ggtctggaat tacgtaagta
     7501 ttacgttcga cgatccgagc gggtatccga atgtactcgg cgtaagcgcc attgttgaac
     7561 agtaggtcct cacaaagatt ctcgagtccc cttcggcagt aatagcactg gtaacatgga
     7621 gcggagttcg ccgcgaccac acgctggccc accctaaact tcctgacgcc tgagccagcg
     7681 gcgacaatat caccagcgag ttcgtgcccg aaaacggcgg gcggcacaat catgcgggca
     7741 tggtacccgc gcaggaagac tttcacgtcg gtcccgcagg ttagagcagc tttgacacga
     7801 acgagtagat caccttccgc aaggtctgga acggctaccg gctctacgcg gagctcttcc
     7861 ttgccgtaca gcacggcagc catcatttgt gtcttcactc ggtccgcctt tgtggtgaaa
     7921 ccataatttt caacgattgt tcgcccggat gcaaggccag ccggatgcca taattgattt
     7981 cgtcgagcgg caggcagtgg gaaatgaggt cctcgacggg cagttcgccg ctaaaaacga
     8041 gcctggcaga ctccgcctgg aggtcgatcg aagcgctgta agagcccagg accgtacgct
     8101 ccccgacaca aatgtcggct ccggagaatt ccacccgctc ctggtgcgat gtctgcgcga
     8161 aaagcaggat acgcgcgcca gggcgcgagc tgcgcatcgc ctgctcggtg atgccgggcg
     8221 cggaagccgc cacgaccacg gcgtcggcgc cacgtccccc ggtgagtgca gccacctcgc
     8281 gcacgacgtc tgcggcgcgg ggatcccata cggactggga gccgaagcgc accgacagct
     8341 cgcgccggcg cagaatcgtg tcggtcgaaa cgatcgccgc gccggtccgc cgaacgagca
     8401 tggtgaacat caggccaatc ggaccctggc cgaggatcgc taccagctcg ccaggctgcg
     8461 gattaaggga ctggatagct ttcaggcagg tgtttaccgg ttccacgaac gcggcgcgct
     8521 caaaaggaac gccctccggt atcctttcca ctcccctccg tacgatccag tccattacgc
     8581 gaacgtactg cgagaagccg ccgccggcgg gctcgaaccc cgcagtgacg ccgactttct
     8641 tgtacacagg gcactgcgcg tagagctttt tccggcagta gaagcactcc gtgcacggga
     8701 tgtgatggaa gacgatcacc cggtcgccgg gcgtgtactc gcgcacaccc tcccccaccg
     8761 ccgccaccac gccggcggtc tcgtggccga aaatcctcgg aggagccaac aggtcgtatt
     8821 cgatcttctt caaatcggtg tggcagacgc cgcacgcctc gacccgcacc agcagttcgc
     8881 cggggccaat gacaggagtt gttacgcgct ccaccgaaac ggtgccattc cctttataga
     8941 ctgccgcacg cattgtctcc ggcggcgtcg taagatcaga atcgacagtc tgcaagaaag
     9001 tctcctaggg accgcgcaac cagcttgctg cgccggtgca gccgataaag ctccggaact
     9061 cgccgaaaag gccgggcgag cgcagcccgg acgatccgtg ccgtccgcag ccggccgctg
     9121 ctatcccgaa cacgattgaa atccgtatcg aactcctcca tcgcagtatc gagcaccgcc
     9181 cgaacacaat agaacggcaa cgaggcgccg gcggctctcg ccgcgagccc ggcggactcc
     9241 atctccacca cggaagcacc acacgcgcgc aacgcccgct tttcagcagc cgtccggacc
     9301 acacgatcca ctgagaacag gacgccgatg gccttggcct gggggctgcc gggcacttca
     9361 gtccggtagg acccgccacc gccggcgtcc tccactcgcg acgcgacgaa tacgtcgcct
     9421 gcttcgagcg acggatcgag cgcaccgcat gaccctgtgc tcacgaccct gtccagcttc
     9481 ccgttccggg cggcaacatc gaaggcttcc gccgcgcgtt cgggacccgt tccgttggca
     9541 gccagtacca gcctgtatcc attcagttct gccagacgca cgaaatccag cggccagccg
     9601 gggttctcca tctttccgca ccgccggact aacccgacca actccctgac ctcggcagcg
     9661 accaccagga tcgtttccac gtcagcagta gccgttctgg cggaaccatt ccaccgcccg
     9721 ccgcaacgcg ccatcgaccg cctgcggggt gaatccaagt tcccgcgcgg ctttatcgtg
     9781 ccgcacaaac atctttttcc gcgccatccg cactgcgtcg aacgggatgc gcgggtcccc
     9841 tcccgtcagc cgcgcaaggc ccgtgctgag gaccccggca gtccatgcca ccccatacgg
     9901 aatccttgtg ctcggcgcct tcaacccgct cgccacacca agcttccgta aaatctccga
     9961 gagagtcagg ttctcacagc cgaggacgta ccgctcccca acccttcccc gctcgcaggc
    10021 cagccggtga ccttcagcgg tgtcccgcac atccacaagg ttcagcccgg tgtcgacgaa
    10081 cgccggcatc gctccgcgca gaaagtccac gacgatcttg ccggtaggcg tcggcttgaa
    10141 gtcgtggtcc ccgaccggcg ccgtgggatt gacgatcacc accggaaacc ccgaccgcga
    10201 gtattccagc gctacctgct cggccagaaa cttcgttcgc ttgtacgccc cgaccatatc
    10261 atgcaggccg accggtgtgt cctcgtcgcc cagacaggtc gccgggatgc cgatgcagcc
    10321 caccgtactt gtatatacga aacgttcaac gccggccctt cgcgctgcct caagcatgtt
    10381 ccgcgtgccg tccacgttgg aacgatgcat ctcttgtggg tgcttcgtcc aaaggcgata
    10441 gtccgccgcc acgtggaaga ccacgccaca tccgcaggcg gcgcgctgca atgactcggg
    10501 atcgcgaagg tcgccggtgg ccgtttccgc ttccaactcc cggagccggc tttccggtct
    10561 taccagcaca cgaatgcgat ggccgcgttc aataaggttc cgcgccacgt gccacccaac
    10621 gaagccggta gcacccgtaa taagggtcgg tctcacgtcc tttcgttatc gcaaggccca
    10681 gctcagcaga cgcaggctgt cactggcctt tccgttgatt cccaatgccg ccgacggttc
    10741 gtagccgcaa tgcatcatac aatgttcgca gcgggggtcg ttaccgtggc cgtagtcctc
    10801 ccacggcgtt ctgaccataa attcttcaaa agtctcgtag tgcccgtccg taatgaggta
    10861 gcagggccct ttccagcccg aaacattgta agtgggattg ccccatgcgg tacagggcag
    10921 gtcgcgcgta cccttcagaa agtcgacgta gacgggcgtg ctgttcaaac ggaaccgccg
    10981 cgccagccgg tcgatgtcct tgaacttctc gtgcacatcc tcgcgggtca tgaagatctc
    11041 gcggtcattg accgccgagt agccgtagcc cggcgcaagc atgtgcccgt ccactccgaa
    11101 cggctcaagg tactcgaaga gggcctcaat ctccttcatg tcggtctctt tgtagacggt
    11161 cgccctacac tcccgaccgc gaaggcttct tcgtgtttcc ggccgacggc ggcagcaaca
    11221 acacccgcgt ctacgtgatc acgaccgcca ccgcggaagt gaaggcgggg ccgacggcgg
    11281 aagctctggc tcagcgcttc gtgcgggact agcggggccg ccaccgagcg ggccacccgg
    11341 gcggccgctt gcctccccgc ccgccgctcg atactttgtg
//
LOCUS       DATIFQ010000280         4243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2773093, whole genome shotgun sequence.
ACCESSION   DATIFQ010000280 DATIFQ010000000
VERSION     DATIFQ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773093"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VLE22_13240"
     CDS             <1..394
                     /locus_tag="VLE22_13240"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HSB15418.1"
                     /translation="ITVRDGKGGKQRIVPMSIRLRKILYVYVQKHVSLTGLVFYACDG
                     VKACQNNLRRDLGAVTRRLRITGVKGGYHVLRHTFAVNYLRAGGNVFYLQRILGHSTL
                     EMTNRYVRSLGIEDLQAVHNRLSLLSRS"
     gene            complement(607..1080)
                     /locus_tag="VLE22_13245"
     CDS             complement(607..1080)
                     /locus_tag="VLE22_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15419.1"
                     /translation="MKVPLQLWNRPHWELRGRWDSAQFFRHLLEVLPAATTLFIEGTC
                     IAREVSDLMRSASEPGDYLPMRQTLWPRARQYRLRCDSATLAALADLAERHAAPELLD
                     HLFVYDGPKVLLEFPDAFVRDCPAYFSTETDEQSLRSFAAVLRLDLTNLQSGGRG"
     gene            1228..1434
                     /locus_tag="VLE22_13250"
     CDS             1228..1434
                     /locus_tag="VLE22_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15420.1"
                     /translation="MRCAVLLLASVPLLGQPGPQLERIAARLSYFCAQNFFYDLPGCF
                     GQREEDYLKSVAKGLCLYKRQSNN"
     gene            1461..1850
                     /locus_tag="VLE22_13255"
     CDS             1461..1850
                     /locus_tag="VLE22_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSB15421.1"
                     /translation="MPSDAGYEDARRVYNGMIDKRPGAIARCMNVADVIAAVRAAREQ
                     DVLTAVRGGGHNAGGLGICNDGLVIDLSRMKGYEWIPPRALCAWKGAAYGLMWITPRT
                     PLAWPLRAERLAARASPASRWAAASAT"
     gene            1895..2053
                     /locus_tag="VLE22_13260"
     CDS             1895..2053
                     /locus_tag="VLE22_13260"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HSB15422.1"
                     /translation="MVLADGSFVTMMEDGQERAKASFGKNYDRLAAIKKKYDPTNFFR
                     VNQNTRPA"
     gene            complement(2169..>4243)
                     /locus_tag="VLE22_13265"
     CDS             complement(2169..>4243)
                     /locus_tag="VLE22_13265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB15423.1"
                     /translation="CLGRAFVPEEDRAGAGKLAIVGYGLWQNRFGADPGLIGRTITLN
                     GQGYRVIGVMPPDFGFPKGAEVPSYYRFAPRTDLWVSASLTQREIDNGSLGFTVVARL
                     KPGISVKQAQGEMSIIMRQLEKEDPNNKGFSVRVIPLHETIVRGLRPALLAIMAAVAA
                     VLLIACANLANLLLARAVARRKEIALRAALGASRWQLIRQLLIESLLVSLAGGALGLL
                     LARWGVVVLERMIPAGLPRVGDARLDSIVVAFSLLVSVLTGVLFGLAPAWNTAKLDLN
                     RWLKEGGRSSAAQAHPLVRRGLVVSEIAVATVLLVATGLLLRSFLKLQSVDTGFRAEN
                     VVAVELSLPGSGFFERGNGRYDQPGRKAEFVTRLVERASALPGVTSAGAISELPLAGG
                     VNMEGLTFEGRPQPDPSRPPVADFRSVTPGYLRTMGIRLLKGRWFTDQDRADSLPVAV
                     IDDTMARTYWPAEEPIGKRFRNLNSSKPWWTVVGVVRSVTHNGLGADKQPEYFRPYSQ
                     QELSWTTLVIRSAIHPPSLLAGLRKEIWAVDKDQPVSKITTLEQVVSEAAAAPRFRSV
                     LIGIFGAFALLLATLGIYGVISYSVAQQTHDLGIRIALGAGSREILSLVLGGGLALAA
                     TGVAIGLAASIGLTRLLASLLYGVTPTDLWTYGGVTVLLLTVALVASYVPARRAMRVD
                     PLVALHYE"
BASE COUNT          842 a         1349 c         1328 g          724 t
ORIGIN      
        1 catcacagtg cgagacggca agggcgggaa acagcgaatc gtgccaatgt cgatccggct
       61 gcgcaagatc ctgtacgtct acgttcagaa gcacgtttcc ctgacgggct tggtgttcta
      121 cgcctgcgat ggtgtgaagg catgccagaa caatttgcgt agggacctag gggcggtcac
      181 ccgccgactg aggattaccg gcgtgaaggg cggctaccac gtcctgcgcc acacgtttgc
      241 ggtcaactac ctccgggctg gtggcaacgt cttctacctc caacgcatcc tcggccacag
      301 tactctggaa atgaccaacc gctacgtcag gagcctgggg attgaggacc tccaagccgt
      361 tcacaaccgg ctgtcgctgc tgagtaggag ttaatgctca gaacaagacc gctagggccg
      421 gttggcgttc gtcgcggact tctttggctg aggtcccgcc tgacgccagt ccccctgcct
      481 tttgacccgt cggattcgct ctcaccccca gcctcaaacg caagtctcgg ccagccccaa
      541 ggttgggggg gcgggtccat ccaacggagg agtcggccct cggagggtag gcgtagtccg
      601 tcgctctcac ccgcgaccac cggactggag gttcgtcaga tcgagccgga gcacggccgc
      661 gaaactccga agactctgtt catcggtctc ggtggagaag tacgccgggc aatctctaac
      721 gaaggcatcc ggaaactcga gcaagacctt tgggccgtcg tacacgaaca aatgatccag
      781 gagctcgggc gccgcgtgtc gctccgccaa gtcggccaga gccgcgaggg tcgcactgtc
      841 acaccgaagg cgatactgcc tcgcccttgg ccaaagagtt tgtcgcatcg gaaggtagtc
      901 gccgggctcc gaggcagagc gcatcaagtc gctgacctcg cgagctatgc acgtgccctc
      961 aatgaacaga gtcgtggcgg ctggtagcac ctccaagagg tgtcgaaaga actgggcact
     1021 atcccagcgg ccccgcaact cccaatgtgg ccggttccaa agttgaagtg ggaccttcat
     1081 gactccccta ttgtgcggcc aaactcgccg gtgcggatgc cccaatacgc agggttctgg
     1141 gcgagtcggc accgatccat cggagatgac tgatatgctc cggaacgaca ggcgcctggg
     1201 aggccaggga tatgatggga ggtatcgatg cgctgcgccg tcctgctact cgcgagtgtg
     1261 ccgttgctgg ggcagcccgg tccgcaattg gagcggatcg cggcccgcct ttcctatttc
     1321 tgcgcgcaga acttcttcta cgatctgcca ggctgcttcg gccagcgcga ggaagattac
     1381 ctgaagtcgg tggcgaaagg attatgtctg tacaaaaggc agtcgaacaa ttgaaatcgc
     1441 gattccgcgg gcaaatgcta atgccttcag acgcgggcta tgaagacgcg cggagggttt
     1501 acaacggcat gattgacaag cggccgggcg ctatcgcgcg ctgcatgaac gtcgccgacg
     1561 tgatcgccgc cgtgcgcgcg gctcgcgagc aggacgtgct gacagcggta cgcgggggtg
     1621 gccacaacgc gggtggcctc ggaatctgca acgacgggct ggttattgac ctgtcgcgga
     1681 tgaagggata cgagtggatc cctccgcgcg cactgtgcgc gtggaagggg gctgcgtatg
     1741 ggctgatgtg gatcacgcca cgcacgcctt tggcctggcc actccgagcg gaacggttgg
     1801 cagcacgggc gtcgccggcc tcacgctggg cggcggcatc ggccacctga cgcgtacctt
     1861 cggtctcact atcgacaacc tactgggcgc ggacatggtg ctcgccgatg gcagtttcgt
     1921 gaccatgatg gaggacggtc aagagcgcgc caaagcctcc ttcggcaaga actacgatcg
     1981 cctggccgcg ataaagaaga agtacgatcc gacaaacttc ttccgcgtga accagaacac
     2041 tcgcccggcg taacactcaa ccgcacacca agcgggacac tgaggtcggt acccaggtta
     2101 ggaagcgttg ctgggttgcc agcgagatgg cgtctttcca cgggctttac ccgggtgagc
     2161 gtccgcactt actcatagtg caacgccacc agtggatcta cccgcatggc ccgccgcgcg
     2221 ggtacatagc ttgccaccag tgccaccgtc agcaacagga ccgtgacgcc gccgtatgtc
     2281 cacaggtcgg tcggggtcac gccatagagt agggaggcca gcagccgcgt gaggccgatg
     2341 gaagcggcaa gaccgatcgc gactccagtc gcggcgagcg ccagaccacc tccgaggacg
     2401 agcgacagaa tctcacgact cccggcacct agcgcgatgc ggatcccaag atcgtgcgtc
     2461 tgctgcgcga ccgaatacga gataacgccg tagatcccca gggttgccag cagcagggcg
     2521 aaagcgccaa aaatgccgat cagcaccgag cgaaagcgcg gtgcagcggc cgcttcagag
     2581 accacctgct cgagagtggt gatcttcgag accggctggt ccttgtccac cgcccagatc
     2641 tctttgcgca ggccggctag cagcgacggc gggtggatcg cgctccggat caccagggtc
     2701 gtccagctta attcctgctg cgaatacggg cgaaagtact ccggctgttt gtcggcgcct
     2761 agtccgttgt gagttacgct gcgtactacg cccaccacgg tccaccaagg tttgctggag
     2821 ttcaggttgc gaaagcgctt gccgatcggc tcttctgccg gccagtaggt gcgtgccatc
     2881 gtgtcgtcga taacggcaac cggcagcgag tccgcacggt cctggtccgt gaaccaccgc
     2941 ccttttagta gccgaattcc catcgtgcgc aggtagccgg gtgtcaccga gcggaagtcc
     3001 gccaccggcg gccgactcgg gtccggctgc ggcctaccct cgaacgtcag gccttccata
     3061 ttcaccccgc cagccagcgg cagctcggag atggcgccgg cgcttgtcac cccaggcagt
     3121 gcagaggcgc gctccaccag acgggtgacg aattctgcct tccggcccgg ctgatcgtag
     3181 cggccattgc cgcgctcgaa aaaccctgag ccgggtagcg agagttccac ggcgacgaca
     3241 ttctccgcgc gaaagccggt gtcaacgctt tgcagtttaa gaaagctcct aagtagcagt
     3301 ccagtagcca ccaacaacac cgtcgccacg gcgatctccg agactacgag gcccctgcgg
     3361 accagaggat gggcttgcgc cgccgaggac cggccgccct cctttagcca gcggttcagg
     3421 tcgagcttgg ccgtattcca ggccggggca agaccgaaca gaactccagt cagaacggag
     3481 acaagaaggc tgaaggccac gacaatcgaa tcgagccgcg catcgccaac tcgcggcagg
     3541 cctgcgggta tcatccgctc cagcaccacc acgccccagc gggcgagcag gagcccaagc
     3601 gcgccacccg caagcgatac cagcaggctc tcgatcagta gttgccgaat aagctgccag
     3661 cggctggcac cgagcgcggc acgcaatgcg atttccttgc ggcgcgcgac cgcccgcgcg
     3721 agcagcaggt ttgccagatt ggcgcacgcg atcagcagca ccgctgccac cgccgccatg
     3781 attgccagca gcgccgggcg caggcctctc acgatcgtct cgtgcaacgg gatgactcgc
     3841 acgctgaaac ccttgttgtt cggatcctcc ttctcgagct gccgcatgat gatcgacatc
     3901 tccccttgtg cctgtttgac cgagatgccc ggcttgagcc gcgccacgac ggtgaagccg
     3961 agtgagccgt tatcaatctc acgctgcgtg agcgaggccg agacccacag atccgtgcgt
     4021 ggggcaaaac gataataaga tgggacctcg gcgcccttcg ggaagccgaa gtccggcggc
     4081 atcactccga tgacccggta tccctggccg ttaagcgtga tcgtgcggcc gatgagcccg
     4141 ggatcggcgc caaagcggtt ctgccacagc ccatagccca cgatcgctag tttgccagcg
     4201 ccggcgcgat cctcctcagg aacgaaggcg cggccgaggc agg
//
LOCUS       DATIFQ010000281         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2582648, whole genome shotgun sequence.
ACCESSION   DATIFQ010000281 DATIFQ010000000
VERSION     DATIFQ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582648"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VLE22_13270"
     CDS             <1..1010
                     /locus_tag="VLE22_13270"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSB15424.1"
                     /translation="FDLILGTEIASGAHGGEAVIGGRLRARGIGCFLAGSDASLGKDF
                     ETLLTSQAASDARARWRPSIVEVVAAKPITPKTPASREVPPDMVAIGPVRFEMTVEYR
                     VRETGFYDPNEFTEGRGYRLHTPAQVSHTADLGRYAINLTPVTNAQYAEFLRRSGYKP
                     ARTENFLKHWTNGAPPAGIEDHPVVYVDLDDARAYAAWAGKRLPSEGEWQFAAQGPDG
                     LRYPWGNEMTPSLCNGGETGGTTPVKAFPGGRSPFGCYDMCGNVWEWTESEHGSGHSR
                     FAMIRGGSYFKAAGSDWYMDGGPQPCRFAAKMLLMWPGLDRCATVGFRCAVDLAPPAA
                     L"
     gene            1156..2199
                     /gene="rsgA"
                     /locus_tag="VLE22_13275"
     CDS             1156..2199
                     /gene="rsgA"
                     /locus_tag="VLE22_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HSB15425.1"
                     /translation="MRLEDLGWDASFSSAFEPFERAGLRPGRISRTDRRSFRVLTGQD
                     ELHAEVSGKLRYHTPEWPVTGDWVVLRGDSPVIDTVLPRKTELSRKTAGEQTGKQVLA
                     ANVDVLFLVTGLDRDFNPRRIERWLLLAGECGAQPVIVLNKADLHPDPDWAAGEVRQL
                     APAVPVTVVSALAEQGAAELIRHVRHGETAALVGSSGVGKSTIVNWLLERDAQLTQEV
                     RKDDGRGRHTTTSRELIPMPGGWLLMDTPGLRELQLWAGEDSLDQTFEDVANFAAMCR
                     FRDCRHEGEPGCAVTEAVSGGDLDEARLGSYRKLQRELAYLARRQDEWAAAEEKRRWK
                     RIHKAMRQGGKRL"
BASE COUNT          407 a          688 c          765 g          382 t
ORIGIN      
        1 gcttcgacct gatcctcggg acggagatcg catcgggggc ccacggtgga gaggcagtga
       61 tcggaggccg gctccgcgcg cgtggcatcg gctgtttcct cgcgggctcg gacgcctcgc
      121 ttggcaagga cttcgagacg ctcctcacga gccaggcagc gtccgacgct cgggcgcgat
      181 ggcgtccgag catagtggag gtagtcgcag cgaagcccat aactcccaag acgcccgcaa
      241 gccgggaggt tccgccggac atggtcgcaa tcgggccggt gcgtttcgag atgaccgtcg
      301 aataccgtgt acgcgagacc ggcttctatg atccgaacga attcaccgag ggacggggct
      361 atcgcctgca cacaccagca caggtgtcgc acaccgcgga tctggggcgg tatgccatca
      421 acctgacgcc ggttacgaat gcgcagtacg cggagttcct tcgccgctcc ggctacaagc
      481 cggctcgcac ggagaacttc ctgaagcact ggacgaatgg cgcgccgcct gccgggatag
      541 aggaccatcc ggtggtttac gtcgatctcg acgacgcccg tgcgtacgcg gcgtgggcag
      601 ggaagcggct cccgagcgaa ggggaatggc agttcgcagc gcaaggtccg gacggtctca
      661 gatacccctg gggaaacgaa atgacaccga gcctctgcaa cggcggcgaa acaggcggca
      721 ccacgccggt gaaggcattc cccggcggca ggtctccttt cggctgttac gacatgtgcg
      781 gaaacgtttg ggagtggacc gaaagtgagc acggttccgg ccattcacgt tttgccatga
      841 ttcgcggcgg ctcctacttc aaggccgccg gctccgactg gtacatggac ggcgggccgc
      901 agccctgccg gttcgcggcc aagatgctgc tcatgtggcc cgggcttgac cgctgcgcca
      961 cggtcgggtt ccgctgcgcg gtggacttgg ctccgcccgc ggcgctatga ttcgaagttg
     1021 gtattgcctc tgacagtcct tgcctaaacg ctgaatctct gcgccgcaca cgttcgtagt
     1081 gtgggctccg ggggttcagc ctctccttcc tgtaagtcac ttaaatctct gtgaaaggaa
     1141 atggcaggtt ctgtcatgag gctggaagat ttgggttggg acgcgtcctt ctcgagcgca
     1201 ttcgaaccct ttgagcgcgc gggcttgagg cccgggcgca tctcgagaac cgatcgccgc
     1261 tctttccgcg tactcacagg acaagacgag ttgcacgcgg aggtcagcgg gaaactgcgc
     1321 tatcacactc cggagtggcc ggtcaccggc gattgggtcg tgctgcgcgg ggattcccct
     1381 gtcattgata cggtgctccc gcgcaagacg gagctttccc gcaagacggc gggtgagcag
     1441 accgggaaac aggtcctggc ggcgaacgtc gacgtcctgt ttctagttac cgggcttgac
     1501 cgcgacttca acccgcgccg catcgaacgc tggctgcttc tcgccggcga atgcggcgca
     1561 caaccagtca tcgtcctgaa caaagccgac ttgcatcccg acccggactg ggctgccggc
     1621 gaggtgcggc agcttgcgcc ggccgtaccc gtgacggtgg tcagcgcgtt ggcggagcaa
     1681 ggcgccgcgg aactcatccg ccacgtgagg cacggcgaga ccgcagcgct cgtgggctct
     1741 tccggggtag ggaagtcgac catcgtgaac tggttgctcg agagagacgc gcagctaacc
     1801 caggaggtgc ggaaggacga cggcaggggt cgccatacga ccacgagccg cgagctgatc
     1861 ccgatgccgg gcgggtggtt gctcatggat acgccgggcc tgcgcgaact gcagctctgg
     1921 gctggcgagg acagtctgga ccagacgttt gaggacgtgg cgaatttcgc agccatgtgc
     1981 cgcttccgcg actgccgtca cgagggcgaa cccggatgcg ccgtaacgga agccgtctcc
     2041 ggcggcgatc tggacgaggc gcggctcgga agctaccgta agctccagcg cgagctggcg
     2101 tacctcgccc gccggcagga cgagtgggca gccgcggaag agaaacggag gtggaaacgc
     2161 atccacaagg cgatgcggca gggcggcaag cgactgtaga ttcagcgaca atcacctccg
     2221 gtaatccttt gcgacgggct gc
//
LOCUS       DATIFQ010000282         5095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2390011, whole genome shotgun sequence.
ACCESSION   DATIFQ010000282 DATIFQ010000000
VERSION     DATIFQ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390011"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VLE22_13280"
     CDS             <1..594
                     /locus_tag="VLE22_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSB15426.1"
                     /translation="GSSGFGEEFAAAINKDPLQRPAAEINQAADEAIRRFPFIDAKRQ
                     VAGGASYGGYLANWMEATTDRYLTLFNHAGLTNNESMWGTTDASYYWERKNGSPVWEG
                     GGFWQSQNPLRFAAQYKTPMLVTHGEGDYRVPVSQGFEIFKLLQRRGIPSKLILFPEE
                     NHWILKGENNRYFFRELFAWLEKYTGVGLKEPNASSN"
     gene            614..2239
                     /locus_tag="VLE22_13285"
     CDS             614..2239
                     /locus_tag="VLE22_13285"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HSB15427.1"
                     /translation="MRFFCLTCTGGLLATLLASGIAAEPLNLIPAPRSVNHGAGAYVL
                     RSPVRISVVSGSEDDRFAAGLFARELKEVHGLEATVVSQPGGPVLIGRTGTPAIDAEI
                     MRRKLDISALQHPESYLLDAGANGVLVAAKTAEGIYYGVQTLRQLAGTGGRVPNVRIA
                     DWPALRYRALSIDINRGPLLTEEQMKQAVETAAEFKFNMVFFYLEHVFRYTHSALAAP
                     RGGEAVTWAYSPLENYSKYIEPFRKSGMDFMVCTAVHNWNRLFPAFATTRPNVNVFAR
                     DGKNTGAMGLMATHWADDGEALFNMTWYGVVFSAAAAWQDGLVDTEVFDRSFDWAFYR
                     NRDHTFVGAIRRLEQVHELLRSVRAGEANSTLYWTDPFSRDGAEVMHRARPVASKMRL
                     PAEEAALDLLIHGGTARHHAGTIRFLELAAKRIDYLGMKIQFANEIGELYRELRAAPA
                     DDALATNNLRRIKGMDGLLPTLRDYSTEVKAAYRDAWLAENRPYWLDNVLLDYDREAL
                     RWVDRIRFFESIHRRYPAAREIPDLEPMGVFLP"
     gene            2317..3642
                     /locus_tag="VLE22_13290"
     CDS             2317..3642
                     /locus_tag="VLE22_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15428.1"
                     /translation="MDHSHGVDTATTPSRRSFLKTAVAASTAGLAATPGYVAASDTIR
                     VGVIGCGGRGTEAAEQAMAADKGVRLVAMADLLIDRAQEKRNLLRMKNREQVMVDDDH
                     CFSGFEGYKKVIESSDVVLIANAAKFHPFHMTAAIEAGKHVFVEKPHAIDPAGIKAVR
                     AACELAKQKNLCVVSGLQSRYHPGYRETIQRVYDGAIGDIVAIEENFLRAPYVLYPRK
                     PGQTEVQYQGSNQYHFHWLSGDDVPQSLVHNLDRASWAMHNQAPVKCHGMGGRSTLHG
                     EIYGSVFDHHAVVYEFANGVRIYAFCRTIPECYNDSSSTLLGAKGRCNITKMTIEGET
                     KWQYAGPRTYSSTSSNPYYIEHAELFKAIRAGTPLNNGDYMARSTLMGIMGQISCYTG
                     KEVTWEQISASDFYFAPKPEDVRIDMEPPVKPGPDGTYPVFTPGVTKLL"
     gene            complement(3614..>5095)
                     /locus_tag="VLE22_13295"
     CDS             complement(3614..>5095)
                     /locus_tag="VLE22_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HSB15429.1"
                     /translation="VAETYGIGNISLRYIGWGAEFVDFDSDGWLDLAVANGSTFETQG
                     QPRKLVPMEPFLFWNNHGKFFFDLAPWNTSLSVAHVSRGLAVSDYDHDGAADIAVMDL
                     DGGVRLLRNRIPQGNWIEFRLHSRTAGGILGRGEGARLIAEAGGQTFRHSVTTASYLS
                     QSSRTVHLGLGQAPRVDALEVRWPDGSAQHFASLAPNQIWDLTQGKTEALPWNPGVGG
                     ELLSRTQSPQHNVGSKQAEIQFWSKQRAAMDAMKREGDIPRAISLFREALALNPAHED
                     SRYYLANCLLVRGDLWGAVRELDELARLNPSSHRAFQRKGLLLASAARSDSELSKAQE
                     SLEKAHSLNPEETGTRLLLGELSLLRGDLAGARQRFELVCRSHARSVGALYLRAYLAW
                     KRGDRQGSEDLLKAAAAARGKDWKPKGSVMEGDVLKRMHTDATLLSRFWDEWDGTANP
                     TRAFGALDAFVRLPVAMRRSADSSPQSDRSEPARVTAVSSRRE"
BASE COUNT          994 a         1538 c         1643 g          920 t
ORIGIN      
        1 gggtcgagcg gcttcgggga ggagttcgct gcggccatca acaaagatcc cctgcaaagg
       61 ccggccgccg aaatcaatca ggccgcggac gaggcgatcc gccggttccc gttcatcgac
      121 gcaaaacgcc aggtggccgg cggtgcgagc tacggtggtt atctcgcgaa ctggatggaa
      181 gcgaccacgg accgctacct gacgctgttc aaccatgccg gattgacaaa caacgagtcg
      241 atgtggggga ccaccgacgc atcgtactac tgggagcgca agaacggcag cccggtatgg
      301 gaaggaggcg gcttctggca gagccagaac ccgctccggt tcgccgcgca gtataaaact
      361 ccgatgctgg tcacgcacgg cgagggtgac tatcgcgtgc ccgtctcaca gggcttcgag
      421 atcttcaagc tgctccaacg ccgcggcatt ccgagcaagt tgatcctctt cccggaggag
      481 aaccactgga tactgaaagg cgagaacaat cgctatttct tccgggaact cttcgcttgg
      541 ctcgagaagt acacgggcgt cggcctgaag gaacccaacg cgagttcgaa ctgaacacgg
      601 aaagaatcga ttgatgagat ttttttgttt gacctgcacc ggcggactgc ttgccacgct
      661 gctcgcctcc ggtatagcgg ccgagccatt gaaccttatc ccagcgcccc ggtcggtgaa
      721 ccacggcgcg ggggcgtatg tgttgcgatc gcccgtccgg atttcggtcg tatccgggtc
      781 tgaagatgac cgcttcgccg cgggcttgtt cgccagggag ttgaaggagg ttcacgggct
      841 ggaggcgacg gttgtttcgc agcctggcgg cccggtgttg atcggccgca cgggaacgcc
      901 ggcgatcgac gccgagatta tgcggagaaa actggacatc tcggcgctgc aacatccgga
      961 aagctacctg ctggatgcgg gcgcaaacgg agtgctggtg gccgcgaaga cggcagaggg
     1021 catctattac ggcgttcaga cgctgcggca actcgccggc acgggtgggc gcgtgccgaa
     1081 cgtgcgtatc gccgactggc ccgctttgcg ttaccgcgcg ttgtcgatcg acatcaaccg
     1141 cggtccgctc ctgacggaag agcagatgaa acaagccgtc gagaccgcgg ccgagttcaa
     1201 gttcaacatg gtctttttct atctggagca cgtcttccgg tacactcact cggcgctcgc
     1261 ggcgccccga ggcggcgagg cggtcacctg ggcctattct ccgctggaaa actactcgaa
     1321 gtacatcgaa ccgttccgga aaagcggaat ggacttcatg gtctgcaccg ccgtgcacaa
     1381 ctggaaccgt ctcttcccag cgtttgcgac gacgcgcccg aacgtgaatg tcttcgcgcg
     1441 cgacgggaag aacacgggcg cgatgggctt gatggcaacc cactgggcgg acgatggtga
     1501 agccctgttc aatatgacct ggtacggcgt ggtcttctca gcggcggcgg cctggcaaga
     1561 cggactggtc gacaccgagg tttttgaccg atccttcgac tgggcgttct acagaaaccg
     1621 ggaccacacg ttcgtgggtg ccatacgccg gctcgagcag gtgcatgaac tgttgcgatc
     1681 cgtgcgtgcg ggcgaggcga acagcaccct ctattggacc gatccgttct cccgggatgg
     1741 cgccgaggtg atgcacaggg cgcggccggt ggcgtcgaag atgcggctcc cggcagagga
     1801 ggcggctctc gatttattga tccacggggg tacggctcgc caccatgcgg gaaccatccg
     1861 gttccttgaa ctcgctgcga aacgcatcga ctatctcggc atgaagatcc agtttgcaaa
     1921 cgagatcggg gagttgtacc gggagttgcg ggctgctccc gccgacgatg cgctggcgac
     1981 aaacaatctg cgacggatta agggcatgga cgggctgctg ccgaccctgc gggactactc
     2041 gaccgaggtg aaggccgcct atcgggacgc gtggctcgcc gagaaccggc cgtattggct
     2101 ggacaacgtg ctgctggact acgaccgtga agctctccgc tgggttgacc gcattcgttt
     2161 cttcgagagc attcaccgga gatacccggc agccagggaa atcccggatc tcgagccgat
     2221 gggcgtgttc ctgccctgaa acgtccggga gcgattacac ttgttgcgac agaggcaggg
     2281 ccggggctaa cggcccgtta gaggagaagc cagacgatgg atcactcaca cggagttgac
     2341 accgcgacga cgccttcgcg ccggagcttc ctgaaaaccg cagtagcggc ttcgaccgcg
     2401 ggattggcgg ccacccctgg ctacgtcgcc gcgagcgaca cgattcgcgt cggcgtaatc
     2461 ggttgcggcg ggagaggcac tgaagccgcg gagcaggcca tggcagccga caagggcgtt
     2521 cgtcttgtcg caatggccga tctgctgatc gaccgcgcgc aggagaaacg caatctcctc
     2581 cggatgaaga atcgcgagca ggtcatggtg gacgacgatc attgcttttc cggcttcgag
     2641 gggtacaaga aagtcatcga atcctcggac gtcgtgctca tcgccaatgc ggcaaagttc
     2701 catccgtttc acatgacggc ggcgattgag gcgggtaaac acgttttcgt cgagaagccc
     2761 catgcaatcg acccggccgg gatcaaagcc gtgcgagccg cctgcgaact cgcgaagcag
     2821 aagaaccttt gcgtcgtctc gggcctgcaa agccgctacc atccgggtta ccgggagacg
     2881 atacagcgcg tgtacgacgg cgccatcggg gatatcgtcg cgatcgagga gaacttcctt
     2941 cgcgcgccct acgtgctgta tccgcgcaag ccgggccaaa cggaggtcca ataccagggc
     3001 agcaatcaat accacttcca ctggctctcg ggcgacgacg tgccgcagtc gctagtacat
     3061 aacctggacc gcgcgagctg ggcgatgcac aaccaggcgc ccgtgaagtg tcacggcatg
     3121 ggcgggcgat ccaccctgca cggggagatc tatgggagcg tgttcgatca ccatgcggtc
     3181 gtctatgagt tcgccaacgg cgtgcgaatc tatgctttct gccggaccat accggagtgc
     3241 tacaacgatt cctccagcac gctgctgggg gccaagggtc gctgcaacat aactaagatg
     3301 accatcgagg gtgagacgaa gtggcaatac gcgggaccca ggacctattc gagcacgtct
     3361 tcgaatccgt actacatcga gcacgccgaa ctgttcaagg cgatccgggc tggaaccccg
     3421 ctcaacaacg gtgactacat ggcccgcagc acactgatgg ggatcatggg ccagatcagt
     3481 tgctacaccg gcaaagaagt cacctgggag cagatcagcg cctccgactt ctatttcgcg
     3541 ccgaagccgg aggacgttcg aatcgacatg gagccgccgg tgaagccggg tccggacggc
     3601 acctacccgg tcttcactcc cggcgtgacg aaactgctgt gacgcgcgcg ggctcacttc
     3661 tatcgctctg aggcgagctg tccgcggaac ggcgcatcgc aacgggaagc ctgacaaagg
     3721 cgtcgagggc gccgaaagca cgagttggat tggcagtacc gtcccactcg tcccagaagc
     3781 gggagagcag cgtcgcatcg gtgtgcatgc gcttaagtac atcgccttcc atcacgctgc
     3841 ccttgggttt ccaatccttg cctctggcgg cagccgccgc tttcaggagg tcctccgaac
     3901 cttgacggtc gccgcgcttc caggccaggt aggcgcggag gtaaagtgcg ccgacggatc
     3961 gcgcatggct gcggcagacg agttcgaagc gttgccgagc gccagccagg tcacctctca
     4021 ggagggacag ctcgccgagc agcagacgtg tgccggtctc ctcgggattg agcgagtgag
     4081 ccttttcgag cgattcttgc gccttgctta gttccgaatc gcttcgagcg gccgacgcca
     4141 gaagcaaccc tttccgctgg aaggcccggt ggctgctcgg gttgaggcga gccagttcgt
     4201 cgagttcccg gacggcgccc cacaggtcgc cgcggacgag caggcagttt gccaggtagt
     4261 agcgcgaatc ttcgtgcgcg ggattgagcg ccagggcttc tcgaaagagg ctgatggcgc
     4321 gcgggatgtc gccttcgcgc ttcatcgcat ccatcgccgc gcgctgttta gaccagaact
     4381 ggatttccgc ttgtttcgag cccacgttgt gttgcgggga ctgcgtgcgg ctcaagagct
     4441 ctccgccgac acccgggttc cagggcaacg cctccgtctt accctgcgtg aggtcccaga
     4501 tctggtttgg cgcgaggctg gcgaagtgct gcgccgagcc gtccggccac cggacctcca
     4561 gggcatcgac cctgggcgct tggccgaggc cgaggtgaac ggtgcggctg gactgcgaca
     4621 ggtagctagc cgtggtcaca gagtggcgaa acgtctgtcc gcccgcctcg gcgatcagcc
     4681 gcgccccctc accgcgcccg aggatgccgc cggcagttct gctgtgcaga cggaactcga
     4741 tccagttgcc ctgcggaatg cggttccgga ggagacgcac gcctccatcg agatccatga
     4801 cggcaatgtc cgcggctccg tcgtggtcat aatcggagac cgcaagcccg cggctgacgt
     4861 gcgccactga gagtgatgtg ttccatgggg ccaggtcgaa gaagaacttg ccatggttgt
     4921 tccagaagag gaacggctcc atcggcacga gcttcctggg ctggccctgt gtttcaaagg
     4981 tgctcccgtt ggcgaccgca agatcaagcc agccgtcgct gtcgaaatcg acgaactcag
     5041 cgccccatcc gatgtaccga agagagatgt tgccgatacc gtacgtctct gctac
//
LOCUS       DATIFQ010000283         2563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2008558, whole genome shotgun sequence.
ACCESSION   DATIFQ010000283 DATIFQ010000000
VERSION     DATIFQ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008558"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2538)
                     /locus_tag="VLE22_13300"
     CDS             complement(<1..2538)
                     /locus_tag="VLE22_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSB15430.1"
                     /translation="MSTYGDRPAILAFHKHTTETWSFRELADAVMQLAAGLHEQGLRK
                     GEHVALYSPNRPEWILACMALLAAGAIPVPIDSQMASDDLIHVIEDSEARWFMTIRSL
                     TERLTTLGLHRDRSIILLDADENDPRSWQRLRREPGYTSPPVQPEDPALLFYTSGVSG
                     RPKGVPLSHSNLLSNLRALLTVQVYRPDERLLLPLPLHHVYPFMIGLLAPFALGLPVI
                     LPHSLTGPQILRGLREGRVTAIVGVPRLYAALCDAIEERVRQKGRAVSAVFHGLLACS
                     TMLIRHFDIRLGLRLFAPVRNQLAPELRTLVYGGSALAPELAWRLAGLGWQIAGGFGL
                     TETSPILTLIAPGGRHIDTTGTPLPGVTIRIADSDPETGQGEIQARGPNVFAGYRHLS
                     QKTAEAFTEDGWFKTGDLGYFDRNGCLHLVGRASSRITLPGGEKIWPERVEEILDGAP
                     SVRESGVFAREGRLVAVIVPRAVSIHRTEVDNVTRSIRAEIEARLGLLPSYCRLTDFT
                     LSMDPLPRTRLGKIQRHKLNILFHTSKQHRHKLLVEARPISVELMAPEDRQLLEDPVA
                     LQVWNWLAGRFSSVRLTPDTTMALELGLDSLEWMTVTLELRDRVGVDLPEAAISRIGT
                     VRDLLREAVEAEQTTAAAVDPAGQLTNPQELLDPQQQRWLAARGFFLRGLGTSLALLD
                     RLLMRAVFHLEVHGIERVPAEGPWVMTPNHVSLLDPPAIFAALSISSLNRTCWGGWTG
                     IMFRNPVMRLVSRATQVLPIDQSSRPLANLALGAAALARGQNLVWFPEGGRSRDGTLQ
                     AFQPGIGLILTAHPVPVIPVRIQGSFEALPRGAWWPRRSRIRIAFGEA"
BASE COUNT          503 a          776 c          841 g          443 t
ORIGIN      
        1 ggcttcgcca aatgcgatcc tgatccgact tcggcgtggc caccaggccc cacgcggaag
       61 ggcttcgaag cttccctgga ttcggacagg aatcactggg actgggtgtg ctgtcaaaat
      121 cagtccgatt ccaggttgaa acgcctggag cgttccgtcc ctggaccgtc ccccctcggg
      181 aaaccacacc agattctgac ctcgcgcaag ggctgccgcg cccagggcaa gattcgcaag
      241 cgggcggctg ctttgatcta tcggcaagac ttgagtcgcg cggctgacga ggcgcatgac
      301 ggggttgcga aacatgatcc cggtccagcc gccccagcag gtccggttca aggaactgat
      361 ggacagggcg gcaaagatcg ccggcggatc gagcagactg acatggttgg gcgtcatgac
      421 ccagggacct tccgcgggaa cccgctcgat tccgtggact tcgagatgaa ataccgcccg
      481 catgagcagc cggtcgagga gagccagtga cgtcccgagt ccccgcaaaa agaaacccct
      541 cgccgcgagc cagcgctgtt gttgcggatc caacaattcc tgaggattcg tgagttgtcc
      601 cgccggatca acggcggcgg cggttgtctg ctcggcttcg acggcttcgc gcaacaggtc
      661 cctcacggtc ccgatacgcg agatggctgc ttcggggagg tcgacgccga cccggtccct
      721 cagctccagc gtcaccgtca tccattccag cgagtccaga ccgagttcga gcgccatggt
      781 cgtgtccggc gtgagccgaa cggaagaaaa ccggccggcc agccaattcc atacctgcaa
      841 cgccacagga tcttccagca attgccggtc ttccggcgcc atcagctcga ccgagatcgg
      901 ccgcgcttca acgagcagct tgtgccgatg ttgtttactt gtgtgaaaga gaatattgag
      961 cttgtgtcgc tggatcttcc cgagtcgcgt ccgaggcaag gggtccatgc tgagcgtgaa
     1021 gtcggtgaga cgacaatacg aaggcaggag gcccagacgc gcctcgatct cggcccgtat
     1081 cgatcgcgtc acgttgtcga cttcggtcct gtggatcgag acggcgcgcg ggacgatgac
     1141 ggcgaccagg cggccctcgc gagcgaacac accggattcg cggaccgagg gggcgccgtc
     1201 gaggatttcc tccacccgct caggccagat tttctctccg ccgggcaagg tgatgcggga
     1261 ggaggcgcgc ccgaccagat gaaggcagcc gtttcggtca aagtaaccca gatcgcctgt
     1321 tttgaaccag ccgtcttcag tgaaggcctc ggccgtcttt tgcgacagat gccggtagcc
     1381 ggcgaacaca ttgggacccc gggcttggat ttccccctgg ccggtctccg gatcggagtc
     1441 ggcgatgcgg attgtcacgc cggggagcgg tgtgcccgtc gtgtcgatgt gtcggccgcc
     1501 gggagcgatg agcgtgagaa tcggcgaggt ttcggtgagt ccgaagccgc cggcgatctg
     1561 ccaccccaat ccggccaatc gccaggccag ttcgggcgcc agcgcggatc cgccatacac
     1621 cagcgttctg agctccggcg cgagttgatt gcgtaccggc gcgaacagcc gcaggccgag
     1681 ccggatatcg aaatgtcgaa tcagcatcgt ggagcatgcg agcagaccgt gaaagacggc
     1741 ggaaacggct cgccccttct gccgcacccg ctcttcgatc gcgtcgcata acgcggcgta
     1801 gagacgaggg acaccgacga tcgccgtgac acgaccttcc cggagtccgc ggagaatctg
     1861 aggcccggtc aacgaatggg gaaggatgac gggcagcccg agggcaaagg gtgcaagcag
     1921 tccgatcatg aacggataca catggtggag cggcaacggc aagagcagcc gttcatcagg
     1981 gcggtacacc tgcacggtca gcagcgcccg gagattggag aggaggttgc tgtggctcag
     2041 cggtacccct ttgggcctgc cgctgacacc ggaggtataa aacaagaggg caggatcctc
     2101 cggttgcacc ggagggcttg tataccccgg ctcacgacgg aggcgctgcc agcttcgggg
     2161 atcattttcg tcggcgtcca acaagatgat cgagcgatcc cgatggaggc cgagggtcgt
     2221 taagcgctcc gtcagggagc gaatggtcat gaaccaacgg gcctcgctgt cctcgatcac
     2281 atggatgagg tcgtcactcg ccatctgcga gtcgatgggc accggtatcg caccggcagc
     2341 gagcagggcc atgcaggcga gaatccattc ggggcggttc ggtgaataga gcgcaacatg
     2401 ctcgcctttc ctgaggccct gctcatggag tccggcggcc agctgcatga cggcatcggc
     2461 cagctcccgg aaagaccagg tttcggttgt gtgtttgtga aacgccagga tcgccgggcg
     2521 atccccgtag gttgacagcg cacgaacaag ggcctgtagt gta
//
LOCUS       DATIFQ010000284         8497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1242410, whole genome shotgun sequence.
ACCESSION   DATIFQ010000284 DATIFQ010000000
VERSION     DATIFQ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242410"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VLE22_13305"
     CDS             complement(<1..191)
                     /locus_tag="VLE22_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15431.1"
                     /translation="MRDADRNDAGEASDNCAERDGWRLRGMSPKLGRTSEECSGVMMA
                     TKAKASKKRFKTGSKVSLD"
     gene            complement(238..855)
                     /locus_tag="VLE22_13310"
     CDS             complement(238..855)
                     /locus_tag="VLE22_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HSB15432.1"
                     /translation="MRRGRCVMRLSEIRVAGFGGQGVILAAMVIGKAASIFENGHATM
                     TQNFGPESRGGSCSAQLVISDQPVLYPYVSRPDVLVAMSQESYARFTPELKDGGLLLV
                     EQDLVRVSESSRGIRVYGVPATRLAEELGRRMVQNVVMVGFFGAVTRLVDPDALRKAV
                     EDSVPPASRDLNLRAFDKGYDYGMTSAPLVDLAADHDALRQVLEG"
     gene            complement(825..1730)
                     /locus_tag="VLE22_13315"
     CDS             complement(825..1730)
                     /locus_tag="VLE22_13315"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HSB15433.1"
                     /translation="MSTAELEVLEAPNPVEPFLRMDRIPHIWCPGCGIGTSVNCFARA
                     LLESKAHLDRIAVVSGIGCSGRVAGYVELDSFHTTHGRAIPFATGLKLARPDLTVVVY
                     SGDGDLFAIGGNHFIHAARRNVDLKVICVNNLIYAMTGGQSGPTTPYTSVTTTAPYGG
                     FEPSFNLPQLADAAGAVYVARWTTFHVRQLTRAMTEILKKPGFSFIEVLSPCPTLYER
                     RNKLGDGLDTMKYYKEKSKVRNGAPTHEVALGMRDEIVVGKFVDRDRPDYAALMRERL
                     RGQLGERYAEAEAAHCCGGEGALCG"
     gene            complement(1727..2884)
                     /locus_tag="VLE22_13320"
     CDS             complement(1727..2884)
                     /locus_tag="VLE22_13320"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HSB15434.1"
                     /translation="MAADPKGVLTGVHFLDGDQACCEGALAAGARFAAGYPITPSTEI
                     VERFAARIPTVGGTFIQMEDELAASITILGAVWGGAKAFTVTSGPGFSLMMEHIGYAV
                     MTETPCVFVNVQRGGPSTGLPTLPAQGDMMQARWGSHGDYEIIALSPNSPQECFDLTI
                     RAFNLSEQYRTPVMFMMDECVGHMTEKVVIPKPEEIEILPRRHTRKPRSEYKAFETDE
                     DLVPEMVHAGEGYRFHVTGLTHDERGYPSMTPATQDQLVRRLQQKIRKNSGRVDLCEE
                     ENVEDADVVVVSYGITSRVAQRAIEMARAEGLKVGKLRLITVWPFPEARIRELARTAR
                     AFVVPELNLGQMVREVERAAGGAARTLAVTHAGGTVHRPEQILEAIREAAR"
     gene            complement(2874..3122)
                     /locus_tag="VLE22_13325"
     CDS             complement(2874..3122)
                     /locus_tag="VLE22_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSB15435.1"
                     /translation="MAKGQVKILVERCKGCSFCVEFCPTKVLEMSSAFNSKGYHPPHV
                     VAGDKCSGCDLCGMYCPDFAIHGWKVSEMAHSGASNGR"
     gene            3242..5248
                     /locus_tag="VLE22_13330"
     CDS             3242..5248
                     /locus_tag="VLE22_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15436.1"
                     /translation="MQKRALFVLFWTLFGTVSAQQTSVEVRVFIEPAGPKFFVDGTGY
                     SSAAVFFWQPGTRHTLRVAPEQIQGSAGVRSLFQGWNDSSGRYSTSSPEVTVTAGASV
                     TQIVASFQQDFRLHLNFREGRGTVYVNGAAHSRGGEVWVPAGETVRLSAVADPGYVFM
                     GWGSGTGADASSEQVFVMSGARYLYPRFEGAVSVTLTTSPPRLMLAVDRTPMRAPQTF
                     GWGQGTQRTLSPVSPQTDERSGLWVFDSWSDGGADIHLYTVPATNSPVTVTATYAPGA
                     RASFVTVPPGLRLSIDGRENWPAYNFVWGVGSKHTAEAPAEQTGNDGWKYLFRRWSNG
                     GPASQEVTVGEAARTSGVRLVAEYESVGKLLIRSVPGPVSVVVDGADCATPCVLEGET
                     GKEIRIAAPAGARLADGTRVEFESWSDGGAREAVRKLPASPVILTAHYRGVHGLQMVM
                     NAAGPTPEAGVAPGSIISLYGSGLALAVVTGPDNPLSQTLGGVTVLAGDRMLPLFIVS
                     PDQINALLPSDMGEGEYTAVVRREGQADAQASFQVVRNAPGLFTSRDGERVLVTATHE
                     DGNPVTVGNPARPGEMVTVFGTGFGPTNPRPPDGFPVPSEGFVLVDPAEVIVGEERIE
                     PEWACAARGYVGVNAVRFKAPAETAEVKVRFSGKESNAAFLPVK"
     gene            5329..6327
                     /locus_tag="VLE22_13335"
     CDS             5329..6327
                     /locus_tag="VLE22_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15437.1"
                     /translation="MTVPRSSLLIPALLSVFAALPLAGQQVISAQSGLVHYTEGQVLL
                     GDNPVDLKFGQFPQLKENEELRTERGRAEVLLNPGVFLRVAENSGFRMLSTRLTDSRL
                     EFLSGAMVIEAGQLAKDQAVTVLHKDVTVSLAKSGLYRFDSEPPAVRVFEGAAIVETA
                     GGKVEVTKGKMLPFDGSLAAVKFDRDQTDALDNWSGRRAEYLALANVYAARSVADSGM
                     LWRRSGWVFNPYFGMLTYIPWNSVYYSPYGYRFWSPERVYMVYAPPRQSGFGGGGGAR
                     YNSNLGYNTVSPTSAGTSGVMAGARAAAPAPSAPPAASSPAAPIARDSGRAGGRQR"
     gene            6561..8477
                     /locus_tag="VLE22_13340"
                     /pseudo
     CDS             6561..8477
                     /locus_tag="VLE22_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
BASE COUNT         1483 a         2634 c         2838 g         1542 t
ORIGIN      
        1 aagtcaagac taaccttcga gccagtcttg aagcgcttct ttgaggcttt cgctttggtc
       61 gccatcatca ctccggagca ttcttcagat gttcttccga gtttcggtga catgccacgc
      121 aatcgccaac cgtcacgctc cgcacagtta tcgcttgcct cgcctgcatc gttgcggtcg
      181 gcgtcccgca tcctatcacg caacgtggca cagcacgccg ccccagctcc gcggggccta
      241 cccctcgagg acttgtcgga gcgcgtcgtg gtcggcggcc aggtcgacga gcggggcgga
      301 ggtcatgccg tagtcgtagc ccttgtcgaa ggcgcggagg ttcaggtcgc gcgaggctgg
      361 cggcacagag tcctccacgg ctttgcggag agcgtcgggg tctaccagcc tggtcaccgc
      421 gccgaagaaa cccaccatca cgacgttctg caccatccgt cggcccaact cctccgccag
      481 ccgcgtcgct ggcaccccat agacgcggat gccccggctc gactccgaca cccgcaccag
      541 gtcctgctcc accagcaaca gcccgccgtc cttcagttcc ggcgtgaagc gcgcgtagga
      601 ttcctgcgac atcgccacca gcacgtccgg ccgcgagacg tacgggtaca gcaccggctg
      661 gtcggagatg acaagctggg cgctgcacga tccaccccgc gactccgggc cgaagttctg
      721 cgtcatcgtc gcgtggccgt tctcgaaaat cgacgccgcc ttgccgatca ccatcgccgc
      781 caggatcacc ccctggccgc cgaagcccgc cacccggatc tcgctcagcc gcataacgca
      841 ccttccccgc cgcaacagtg cgccgcctcc gcttccgcgt accgctcgcc caactgcccg
      901 cgcagccgct cccgcatcaa ggcggcgtaa tcgggccggt cgcggtccac gaacttgccg
      961 acgacgatct catcgcgcat gcccagcgcg acctcgtgcg tcggcgctcc gttgcgcacc
     1021 ttgcttttct ccttgtagta cttcatcgtg tccagcccgt cgccgagctt gttgcgccgc
     1081 tcgtagagcg tcgggcacgg cgagagcacc tcgatgaagg agaagccggg ctttttgagg
     1141 atctccgtca tcgcccgagt gagttgccgc acgtgaaacg tggtccagcg ggcgacgtag
     1201 accgcgccgg cggcgtcagc cagttgcggc agattgaagg acggctcgaa gccgccatac
     1261 ggggcggtgg tggtgaccga ggtgtacggc gtggtgggcc cgctctggcc gccggtcatg
     1321 gcatagatga ggttgtttac gcagatgacc ttcaggtcca cgttgcgccg ggcggcgtga
     1381 atgaaatggt tgccgccgat ggcgaacaag tcgccgtcgc ccgagtagac gaccacggtc
     1441 aaatcgggcc gggcgagctt cagtccggtg gcgaagggaa tggcccggcc gtgcgtggtg
     1501 tggaacgaat cgagctcgac atagccggcc acacggcccg agcagccgat gccggagacc
     1561 acggcgatgc ggtccaggtg ggccttggat tcgagcagcg cccgggcgaa acagttcacc
     1621 gacgtgccga tgccgcagcc cgggcaccag atgtgcggga tgcggtccat ccgaaggaac
     1681 ggctccaccg gattcggggc ttcgagaact tccagttcag cggtactcat cgcgcggcct
     1741 cccggatcgc ttccagaatc tgctccggcc ggtggacggt gcccccggcg tgcgtcacgg
     1801 ctaacgtgcg ggcggcgcct ccggcggccc gctccacttc gcgcaccatc tgcccgaggt
     1861 tcagctccgg caccacgaac gcgcgcgcgg tccgtgccag ctcccgtatc cgggcctcgg
     1921 ggaagggcca caccgtgatg agccggagct tgccgacctt cagcccttcg gcccgcgcca
     1981 tttcgatcgc ccgctgcgcg acgcgggacg tgatgccgta cgagaccacc acgacgtcgg
     2041 cgtcctcgac attctcctct tcgcagagat cgacacggcc ggaatttttg cggatcttct
     2101 gctggagccg gcggacgagc tggtcctgcg tggccggggt catcgacggg tagccgcgct
     2161 cgtcgtgcgt cagcccggtg acgtggaaac ggtagccctc gccggcgtgg accatctccg
     2221 gcacgaggtc ctcgtctgtc tcgaacgcct tgtactcgct ccgcggcttc cgcgtgtggc
     2281 ggcggggaag gatctcgatc tcctccggct tcgggatgac gaccttctcg gtcatgtggc
     2341 cgacgcactc gtccatcatg aacatcaccg gcgtgcggta ctgctcggag aggttgaacg
     2401 cgcggatggt gaggtcgaag cactcctgcg gggagttcgg gctcagcgca atgatttcgt
     2461 agtcgccgtg ggagccccag cgggcctgca tcatgtcgcc ctgcgcgggc agggtcggca
     2521 ggccggtgga tgggccgccg cgctgcacgt tcacgaacac gcacggcgtc tcggtcatca
     2581 ccgcgtagcc gatgtgctcc atcatcaggg agaagccggg gcccgaggtc accgtgaacg
     2641 ccttggctcc gccccagacc gccccgagga tcgtgatcga cgccgccagc tcgtcctcca
     2701 tttggatgaa ggtcccgccg acggtgggga tgcgcgcggc gaagcgctcg acgatttcgg
     2761 tggagggtgt gatcgggtaa ccagcggcaa atcgcgcacc cgcggccagc gcgccctcgc
     2821 agcaggcctg gtctccatcg agaaaatgga cgccggtcag gacgcctttc ggatcagcgg
     2881 ccatttgagg ctcctgagtg cgccatttcg ctcaccttcc agccgtgaat ggcgaagtcc
     2941 gggcagtaca tcccgcacaa atcacagccg ctgcacttgt cgccggcgac cacgtgaggc
     3001 gggtggtaac cctttgaatt aaaggcggaa gacatctcca ggactttggt ggggcagaac
     3061 tccacgcaga acgaacaacc cttgcagcgt tcgaccagaa tcttgacttg acccttcgcc
     3121 attttcggtg tttcagaccc tccgtaccga ttctaacagc actcgaagtg ccattcctca
     3181 gtggttcgcc cgattgaaga tcggggctgg atcgggaaca gtggattagg tggcactttt
     3241 gatgcaaaaa cgcgctcttt ttgtgctctt ttggaccctt tttggcactg tttcggcgca
     3301 acaaacgagc gtggaggtcc gtgtattcat cgaaccggcc ggtccgaaat tcttcgtcga
     3361 cgggacgggg tactcttcgg cggcggtctt tttctggcag ccgggcaccc gccacacgct
     3421 ccgcgtggcc ccggagcaga tccaaggttc ggctggggtg cggtcgttat tccaaggatg
     3481 gaacgactcg tcgggccggt attcgacatc ctcgccagag gtcacggtga cggcaggcgc
     3541 gtcggtgacg cagatcgtgg cttcgttcca gcaggacttc cggctccacc tcaatttccg
     3601 cgagggccgg ggcacggtgt acgtgaacgg agcggcccat tcccgcggcg gagaggtctg
     3661 ggtgccggcg ggggagactg tacggttgtc ggcggtggcc gatccgggct acgtgttcat
     3721 gggatgggga agcggcaccg gcgcggatgc gtcgagcgag caggttttcg tgatgagcgg
     3781 cgccaggtac ctgtatccgc gattcgaggg ggcggtaagc gtgaccctca cgacgtcacc
     3841 gccgcggttg atgctggcgg tggatcggac gccgatgcgg gcgccgcaaa cgttcggctg
     3901 ggggcaggga acgcagcgca cactcagccc ggtgtcgccg cagaccgatg agcggagcgg
     3961 gctgtgggtc tttgactcct ggagcgacgg aggcgccgac atccacttgt acaccgtgcc
     4021 ggcgacgaac tcaccggtaa cggtgacggc gacatacgcg cccggcgcga gggcgagctt
     4081 cgtgaccgtg ccaccgggcc tgcggctgag catcgacggc cgggagaact ggccggcgta
     4141 caacttcgta tggggcgtcg gatcgaagca caccgccgag gcaccggcgg agcagaccgg
     4201 aaacgacggc tggaagtatt tgttccggcg gtggtcgaac ggcggtccgg cgagccagga
     4261 ggtgacggtc ggcgaagcgg cgcggacgtc cggggtgcgg ctggtcgccg agtacgagtc
     4321 cgtgggcaag ctgctgatcc ggagcgttcc ggggccggtc agcgtcgtgg tcgacggcgc
     4381 cgattgcgcg acgccgtgcg tgctggaggg ggagacaggc aaggagatcc ggatcgccgc
     4441 cccggcgggg gcgcggcttg cggacggcac acgggtggag ttcgagtcgt ggtcggacgg
     4501 aggagcgcgc gaggcggtgc ggaagctgcc ggcgtcgccg gtgatcctta cggcgcacta
     4561 ccgtggcgtt cacggattgc agatggtgat gaatgcggcg ggcccgacgc cggaggcggg
     4621 ggtagccccg ggatcgatca tctcgcttta cggctcggga ctcgcactgg cggtggtgac
     4681 gggaccggac aatccacttt cccagacgct cggcggggtg acggtgctcg caggcgaccg
     4741 gatgctgcca ctgttcattg tctcgccgga ccagattaac gcgctgctgc cgtcggacat
     4801 gggcgagggc gagtacacgg ccgttgtccg gcgggagggc caggcggatg cgcaggcgag
     4861 tttccaagtg gtgcggaacg cgccggggtt attcacgtcc cgcgatggcg agcgggtttt
     4921 ggtgacggcg acgcacgagg acggcaaccc ggtcacggtg gggaatcccg cgcggccggg
     4981 ggagatggtg acggtttttg ggacgggctt cgggccaacg aacccacgac cgccggacgg
     5041 ctttccagtc ccatcggagg ggtttgtgct ggtggacccg gctgaggtga tcgtggggga
     5101 ggagcgaatt gagccggagt gggcgtgcgc agcgcggggg tatgttgggg tgaacgcggt
     5161 gcggttcaag gcgccggcgg agacggcaga agtgaaagtg cggttcagcg ggaaggagag
     5221 caacgcggcg tttttgccgg taaagtgact cgcggtcatt tccttgtaac gaaacggagt
     5281 tgaccggcgt ccaagacgcg gaggagaatg gactccaggg agggtgccat gacggtcccg
     5341 cgcagctcgc tcctgattcc ggcgctgttg tccgtgtttg cggcgctgcc gctagccggc
     5401 cagcaggtga tctccgcgca gtccggcctc gtgcattaca cggaaggcca ggtgctgttg
     5461 ggggataacc cggtcgatct gaagttcgga caattcccgc aactgaagga gaacgaagaa
     5521 ctgcgcacgg agcgcgggcg ggcggaagtg ctgctgaatc ccggcgtctt tttgcgcgtg
     5581 gcggaaaaca gcggcttccg gatgctttcg actcgcctga ccgattcccg cctcgagttt
     5641 ctctccggcg ccatggtgat tgaagcgggg caactcgcga aggaccaggc cgtgacggtg
     5701 ctgcacaagg acgtgacggt ttcgctggcg aagagcgggc tgtaccggtt cgactccgag
     5761 ccaccggcgg tgcgtgtgtt tgagggcgcc gccatcgtag agacagcggg gggcaaggtc
     5821 gaggtaacga aagggaagat gctgccgttc gacgggagcc tcgccgcggt gaagttcgat
     5881 cgcgaccaga cggacgcgct cgacaactgg agcgggaggc gggccgagta cctggcgctg
     5941 gcgaatgtgt acgcggcgcg gtcggtggcg gactccggaa tgctgtggcg ccgctcgggc
     6001 tgggtattca atccttactt cgggatgctg acgtacattc cgtggaacag cgtgtactac
     6061 agtccgtatg gatatcggtt ctggagtccg gagcgtgtgt acatggtgta cgcgccgccg
     6121 cggcagtctg ggtttggagg cgggggcggc gcccggtaca attcgaacct cgggtataac
     6181 acggtgtcac cgacgtcggc gggtacatcc ggcgtgatgg cgggggcgcg ggccgcggcc
     6241 ccggcgccat cggctccgcc ggcggcaagc tctccagccg cgccgatcgc ccgcgacagt
     6301 ggccgcgctg gcgggcggca gcggtaggga catcaccttt ctcgctggaa aatgaatcag
     6361 cgatgaccct caaggtcttc ggcgcgatag cgccgggcag gcggtcctcc tcgatgccgg
     6421 agaggattct ggcgcccgcc gcgttcgctc gctcgaagtg cgtatcgagg tcatcaacgt
     6481 aaaccagcac accgagcgac cgcactgact tccgtccagc accgctaccg ggactcgcta
     6541 atggcggtgg ttccagcacc gggttcgctt ttgaggtcac ccgcggggcc gcagtaggct
     6601 acacgcacac ttttactccc acctcgctca acgatctgcg gttgggattc agctatgtca
     6661 acgtccatcg tggcatcccg gaaggcggca ctcaatttcc tccccctgag ctgcaggtgc
     6721 cgggtgtcgt taacaatcca aagaccaacg ggctcaccat attcgcgccg agcggctacc
     6781 ggcgggtggg cgacccaggc tttgctccga cgctgcttgc gagccaagaa cggcagatct
     6841 cggactcgct cagtctgatt cgaggacggc atagcctgaa ggccggcggg caggttcgct
     6901 ggagccagtt caacattttc caggtgtctc gtcctcgcgg aaacttcagc ttcaacggcc
     6961 gctttaccca gaatccagag tcccggtccg gcaccggaag cccgcttgcc gacatgctgc
     7021 tcggcctgcc cagttcctcg actatctctt cgttgctgga cctcggcaac cgccagcagg
     7081 tctacagcat gttcgtggaa gacaactgga aagtgacccc caagctgacc ctgaacctgg
     7141 ggctccgcta cgagtacgcc acgccaattg tcgaagtgaa cgacaagcag agcaatttcg
     7201 atttcgccac gggccagatc gtcgtcgcga accggaacgg acaatccagg ggcttggtcg
     7261 acgtcgacaa gttcaacctc ggcccgcgga tcggctttgc ttatagcccg ttccaggacg
     7321 gcaagacggt cattcgcggt gcatacggca tcttctacag tttgcaggag atcaagactg
     7381 cgggtggctt gcaactggcg tacaacgtcc cgttcttcta tgagcccagc ttcatcagta
     7441 acggaatcac tcccgtactc accgtccccc agggtttccc gccgctcgat cccacgcagg
     7501 ccgtgactcc gcccgtgacc agtctggacc ggcggctaca ttccccttcc tatcagcaat
     7561 ggaacttcgg catccagagg gtactgccca cccagactac gctggaggtc acttatgtcg
     7621 gctccaaggg aacccatctc cagggcctga cggaccacaa ccaaatcgcc ttaccgggac
     7681 ctggggacat ccaggaacgg cggccgtatc cagaatacgg tacgttcacg tcgatggaga
     7741 atcggggcag ctccagctac cattcccttc aggtgaaggc ggacaagaga ctgacgaatg
     7801 gactgatgat tctgagcgcc ttcacctacg cgaaggcgat caacgatcta ccggagatct
     7861 gctgctccca gccttggccg cagaacagct acaacgtccg cgcggagaag ggaagggctg
     7921 attttgacca gcgattccga tgggtgagca gcttcgatta tgagctgccg ttaggaaagg
     7981 gcaggcgatt cgtcaaccat ggaggagcag cggattggat cctgggagga tggcacatcg
     8041 gcggcattgt atccttcacg tcgggctttc cgttttcccc tctgctgggc ttcgatccgt
     8101 ctaacacggg ctcagtggga ctgttgcgcg cgaacaggac agaggatggc aatcttccgc
     8161 ctggtgaacg caaaccggac aactggttca acatcgacgc gtttcctctg ccggatgatt
     8221 tcaccttcgg gaactcgggc aggaacgtgc tggacgggcc cggatcgaag gtgtctgatc
     8281 tatctttccg caagaggttt ctcgaaaaag agcggcaatt tctggaattc cgggcggagt
     8341 tcttcaattt cgctaaccac ccatatttcg cccaacccga taactttatc gacgatggac
     8401 cgggggcggc cggcgtaatt acgggcatct ccgccccgat gcgccagacc cagttcggcc
     8461 tcaagtatgt attctgatcg gccatgactg ctgcaag
//
LOCUS       DATIFQ010000285        22663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_858768, whole genome shotgun sequence.
ACCESSION   DATIFQ010000285 DATIFQ010000000
VERSION     DATIFQ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 22663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22663
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858768"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VLE22_13345"
     CDS             complement(<1..198)
                     /locus_tag="VLE22_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15438.1"
                     /translation="MLGLLGLGAARGLSQSAGLPKRPNILFIYTDDHSYRTISAYPEA
                     YPWVRTPAIDGLARQGVRFTAA"
     gene            complement(283..1380)
                     /locus_tag="VLE22_13350"
     CDS             complement(283..1380)
                     /locus_tag="VLE22_13350"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB15439.1"
                     /translation="MLTGRRDFLSAAVITAGQTRRAPNPAPDKLGIPGPFPGRVVSVS
                     HPGCIDSDKYQQGPVRQMIERGMLEMTGAPTWVDAWRVFVEPGDVVGIKLNPNGAPRV
                     ISAPEVLHAVIDGLRQAGLKPRDIVVFDRNHSFIVRAGYDKWLPEGVRWAWASETYEP
                     IQLDMDGYDPDCYAELPVIQPGQNPNDRHFRRSYVAKFVTKEINKLINIPVLKHHQSA
                     GVTLALKNMAYGMANNVARSHQNSTLHCTSVFIPAIVDLQPFRQKAVLHIVDGVKGVY
                     HGGPSARPQYIWEHKTIYFSTDPVAVDKIGWKALDRKRAEMGMAAIALSKPDKVSTRL
                     NCQTEHIEIAGALGLGEFDDSRIDLRNIELS"
     gene            complement(1395..2339)
                     /locus_tag="VLE22_13355"
     CDS             complement(1395..2339)
                     /locus_tag="VLE22_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB15440.1"
                     /translation="MTRRELLAAGIALAPSLDAAKPIDRLRIAAITDEIGKTPDDAIV
                     FAREYGLRWVELRSVPGARHEYAFLPEHEVKLTAAALAGHDLKVSFLNSSLLKFAWPG
                     TEPVRRRNERPEARAQRLEAEKVRFDRRMDDLRTAIRNAHILGVDKIRVFTGMRVADP
                     RALFPRIAEVIGEMAMVAEKEKVSLAIENEASCNVASSAELAALLEAIPSRHVGLNWD
                     PQNTLGTQEVPFPDGYNILPKKRILNVQIKGKGIMPASSQKLDWKAIFQALARDGYEG
                     KIGLETHIFDGTLIEASHISMKEILRIVGELYGGRMSG"
     gene            2489..3979
                     /locus_tag="VLE22_13360"
     CDS             2489..3979
                     /locus_tag="VLE22_13360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB15441.1"
                     /translation="MSSTRRDLLRRATAMAAAPAIARRLEKRPNILFALADDWGWPSA
                     SIAGDPVVKTPVFDRVARRGVRFTNAFVSSPSCTPSRAAMLTGQWHWRLGEGANLGGT
                     LPVQFPVYPDILEAAGYHVGFTGKGWAPGDEAPGGRKRNPAGSRYRDFTAFLSARPKR
                     APFCFWFGSADPHRPYDRDSGAAAGLRWQDVRVPPYLPDHEIVRRDIADYLLEVQRFD
                     RETGELLAIIERAGELDNTIVVMAGDNGWPFPRAKATIYDPGTHVPLAVSWPSRVSGG
                     REVEDFVSLADLAPTFLEAAGIRPPSQMTARGLLPILTSKRAGRVDPQRSHVLTGMER
                     HVPCRGQALGGYPMRAIRTREFLYIRNFEPGRWPAGDPNGLEDPGAQPFSFDELAGQT
                     FKALADIDAGPTKAYLVTHRSDPGVEPLYALAAGKRPERELYDLREDPFQMSNVAAKA
                     SYTEVVKGLDAQLMAELKASGDPRAYGRGEEFDRYVWYQGQRTGKK"
     gene            3982..4713
                     /locus_tag="VLE22_13365"
     CDS             3982..4713
                     /locus_tag="VLE22_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HSB15442.1"
                     /translation="MHGSPTGGSTPRPHESSFPTRETARCGFGRDWVRVGELLSRCIY
                     EEKSPSPGWGSRTVHRIRPEAASELAAHSVYVEQGKSGKRGAGRRPMFEQMKRDAAQR
                     KFDRLLIWKVSHLGRDMREVIATVYELADLGVSVLPIKSQTGPINSTMGRLLWAIQAW
                     FAEMENDERSEAIRAGHAQARAAGKQIGRPRKVFRRDLVDQQELPFRGFMVFPSMSFV
                     PQRHLYTGDFVKYTAQQRGRHAKVF"
     gene            4886..6214
                     /locus_tag="VLE22_13370"
     CDS             4886..6214
                     /locus_tag="VLE22_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15443.1"
                     /translation="MKFGDYATGLIVKAVRAGVTFLDTGNGYGDSQLNYGAAFRELNV
                     VPGQPGYQAALRSRLFVSTKLGSRYAIIRDASAPTAVGSRGGGARALDQLKLSMTQMF
                     GDGKGSVAEGAYVDMMHVHNLNTEAAVDAVFEGIDNPGDKSLPRVGTLALLIDYRDGT
                     NLTGLNPDHKKWIRHIAMSCHENPTSVMYAMRKDQKNVLEGLMCTINPNDPNYFCFET
                     NALPVAQAKNVGVIAMKVFSDGVFWGKGEHWGGEWVKTIGLPDKVSYQDFIGYTLSSP
                     GVHAAVIGIGEVDKNNDPQRDQFVANLAAFQTARALTLRERKDIMERVAGLHGTHTNF
                     YQRPGSGLQPPQNVSVARPQNGPVEVSWDTAYAADNALVRYEIFRRHDLVARVPFAPQ
                     ITEQPFRFVDDGVKGSFPGGLWYRVRVVDSEGATADSNTILAPQAQGRAG"
     gene            complement(6268..6420)
                     /locus_tag="VLE22_13375"
     CDS             complement(6268..6420)
                     /locus_tag="VLE22_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15444.1"
                     /translation="MKGERYRPSQEHIWNFGLGLVAYTWEEGGPSLAARRLATETNIT
                     HHHDRE"
     gene            6525..7757
                     /locus_tag="VLE22_13380"
     CDS             6525..7757
                     /locus_tag="VLE22_13380"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSB15445.1"
                     /translation="MRPLAVFFAFIAIGLAADKKAVSPPGVTPVGPYSPGVMVGDFLY
                     VSGQGAPGPDGKMPDTLEGQVRQALNNVKAVVEAAGLTMEHVVYTHLYLTDMSTYEQA
                     DKVWAEFFPKAPPARAVVGVYKLPGGTPVEVNAVAFRDLERKKVFTPAGFPAHPGVSP
                     SVMAGQRFFFSSALGMDMASGKVPTNPDEQVQVAFENLKRMLDAAGLDFRHVVMVNPY
                     LAAGSSPAMNKIYASHFEFGNTPGRATIRVTSLPQGNTIAFTGVAIADLSKRLAVRPK
                     NMAPSPTASPCVLADDTLYCSAKSGFIPGPNEGIYSPTVEGQLRQTMRNLLDGLEEAG
                     MNFSNVVATNVYLGDIDEFAKMNRVYAQYFTGVAPTRTTVAQTAPREVAQPPANANRP
                     PNPDTFPGREQISLIAVK"
     gene            7862..9094
                     /locus_tag="VLE22_13385"
     CDS             7862..9094
                     /locus_tag="VLE22_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB15446.1"
                     /translation="MFGYFAAQDLWAMARKAAGRASSADIYARIGVTPIINARGTWTY
                     ISGSLELPEVKAAKQAAAEHFVDMWELQKGVSKRLAELSGAEAGMITAGAASALGTAT
                     AACMAGTDPAKIWQLPDHTGMKYEVIMFGGRLSFDSAIRAAGGVLVMAYSPEELQAKI
                     NENTVMVFTTARGERLEKGLVITKKAGVPMLVDWAAGIPPIENLSLPAKMGADLYTFS
                     GGKGLCGPQCTGLLLGRKDLIEAALANSCPWEGAVCRAHKVGKEEIMGCLAAIEAWKK
                     RDLAKLDREWGARVRRIVQLVETVPGVKTEITTPAGGNRYPTLSVSWDEAAFQMTVLE
                     CAKELRDGRPRIEVLTPTNPSLVPVVREGDPNAPRRRGEDAPRLRIVSLTLQPGEELL
                     VGRRLREVLSEARKKAQG"
     gene            9212..10219
                     /locus_tag="VLE22_13390"
     CDS             9212..10219
                     /locus_tag="VLE22_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB15447.1"
                     /translation="MRQVLLLSLVLLLSPPVSRAAKTLDMYAIDTEGGKALLIIAPSG
                     QSMLIDTGYPGYNGRDTNRIVAAAQAAGVKKLDVLVTTHYDMDHVYNAPSVVAKIPVA
                     LFVDHGQAVKQGASTAKAFAAYLELTAKARRMIAKPGDTVPFKGVDVLVVASNGEALK
                     TPLKGAGATNAACASTPQKQTDKTENGFSLGLLFTYGKFRMVDLGDLSWNRELEMMCP
                     NNPIGTVDLFMVSYHGGDPANSPALIHGLRPRVTIMDNGVKKMGTASVLKTIQSSPGL
                     EAAYQLHWSINAPNDNPPDEFIANLQNSTDGNWIKVSARKDGAFTVTNARTGASRTFR
                     K"
     gene            complement(10310..11359)
                     /locus_tag="VLE22_13395"
     CDS             complement(10310..11359)
                     /locus_tag="VLE22_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HSB15448.1"
                     /translation="MSAADCFAAVMLLPPLVSFAQNYKAEQTTDHGVDIVRLTDAAHG
                     VEVTVVPSIGNRVTEMKVHGKNILYFASTDVGDFKERPKLSGIPFLAPWADLLNEPGF
                     WANGKHYHFNMDLGNVRGNLPGHGLLINSPLWHVTEVASDGHSAHVASRLEFWKYPDL
                     MAQWPFAHEYEMTYGLADGVLEVRITVTNLSTESMPLVLGFHPFFQVPDIPRDEWMAR
                     IPARIHVIPGENNIPTGEMRPMDVPNPLLLKGQKLDDGFTDLERDADGRAHFTIEAGG
                     KTVETLFGPKFTVATVWLPSGPDGQPRPFICFEPLATIISGVNLAHEGKWSGLQTVPA
                     GGKWTESFWIRSSGI"
     gene            complement(11474..11668)
                     /locus_tag="VLE22_13400"
     CDS             complement(11474..11668)
                     /locus_tag="VLE22_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15449.1"
                     /translation="MHPYGEKLRQCSFILPKGFEAQKMTLRAEVETKAGIRRPVRWAC
                     AEPVNPDGSYTFDVPKYAQR"
     gene            complement(11728..13848)
                     /locus_tag="VLE22_13405"
     CDS             complement(11728..13848)
                     /locus_tag="VLE22_13405"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSB15450.1"
                     /translation="MTRRDFFSTTAAFAAPRVLAQDGYPGIAYRRYSRCLPDYLYALA
                     MRAYQARNREIGKLTTVEAIRLRQRWVAETFWKLTGGRPERTPLNARVVGSFDRPGYR
                     LEKIVYESRPGFHIAANFYLPTEGKPPYPGVLFQMGHTLNGKAGDSYQKGCQGLARLG
                     FAVLAFDPMGQGERTYYPRAGGVLTRLSSADEEHTKPGFQMILAGDTSTRLQTWDAVR
                     SLDYLAEHPMVDPKRLASTGQSGGATLTMLLAAVDDRLAAAVVSSGNTENLACAGFLP
                     PGSTDDAEQNFLDGGPAGFDRWDTLYPLAPKPLLVLVSARDYFGTYSPNYLSSGWEEY
                     KKLKKVYEVLGHGDRLEWGDTPMPHNFGYALRLKTYNWFRRWLQDKPEPVREEPPVNV
                     EKDETLWAGPTSNVVRDFDSETPFSLNRKQAERIQTPARPAGIDELLRVEKPAAAPRA
                     SVVGRVSAGPVDVEAIEVPSTSRVFAPAWLFLPRKAETGKPVLIIAEPQGRNSRWREG
                     DLYPSLAEAGLVVCAADVRGTGDLVPEVSPGAAGYQRWHQEEENYAWSSLMLGRPLLG
                     QRVTDLLAFAAALRAHNAARGRRIVLAARGTMTVPALFAAALDPGIAGVYLAGGLVSW
                     RSVVETEDFEYPLANLVYGALSRADLPQVAGSLAPRKVWLAGTVDAAGKRMEPAVVRS
                     AYGSAPNVEVLPDPSWDIAALARL"
     gene            complement(13862..14431)
                     /locus_tag="VLE22_13410"
     CDS             complement(13862..14431)
                     /locus_tag="VLE22_13410"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HSB15451.1"
                     /translation="MTGLLQDMFQLGLPAAEKVLRPVIVYLVLVAFLRIFGKRELAQL
                     NPFDLVVLLSLSNTVQNAIIGNDNSVSGGLLGAFALLGINYVVVRFLFRHRRLDQILA
                     GRPAVLIEHGKVRRDALSRELLTESELLTVLHRQGFSRLNEVERCVLEPGGAFFIEGK
                     APPREERRHDELLRRLDEIGAQIQALRSQ"
     gene            complement(14447..15739)
                     /locus_tag="VLE22_13415"
     CDS             complement(14447..15739)
                     /locus_tag="VLE22_13415"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB15452.1"
                     /translation="MLRRDFLSLPLLSASLYAQRTRGLPPLTIRSVKAIPTSAGGRYQ
                     WVFLKIETSEPGLYGLGSASNVNQALTVATAIEKHLGPFWIGKSADRIEDLWQSTNVR
                     SYWRNSAIQNNVLSALDMALWDIKGKRAGMPVYELLGGKARDAVALYAHADGRDMNEV
                     TENVRKYMDEGYRHVRAQMGGYGGGGMIEPGQGSRPAGGYAGPAFDDELYVDTIPKLF
                     EHLRAKLGKDVKLLHDVHEHLTAIMGVELAKRLEPYRLFFLEDLFPPEQIAWFRQVRQ
                     VTTTPMAMGELFTSPHEWVPLISERLIDFIRCRVSQTGGITSAKKIAALCETFGVRTA
                     FQEGGDNDPINQLASYHVDLSISSFGIQEENHFPPLVHEMMPGTAQLKGGYLYGSDGP
                     GLGIDINEAMAAKYPLEPPPGRDNWTTVRGMDGSLVKP"
     gene            complement(15868..17715)
                     /locus_tag="VLE22_13420"
     CDS             complement(15868..17715)
                     /locus_tag="VLE22_13420"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HSB15453.1"
                     /translation="MFDTLAGLLIEATLNGLWQGILLTALLWLLLRFLPCSAAMRYAA
                     WYACLVAVLSLPAVHVAVSMWPAPSAPAVAIEAPPARITPELVSSSSAPPEGASASVW
                     SPPDLSLPAAAAVLLLAGWVVVALLLGVRLLRSYLGMARLKNNSKPLDDPDTGRWSKW
                     FAACFTRRAFDVRGSSEAPLPMLAGLTRPAILFPESLVPRLTAEEKFRICLHELAHIR
                     RCDDWANLFQRIAETALFFHPGVRWIGNRLNLEREIACDDWVVAVSGSARPYAACLVR
                     LAEYASLTPHPAPALGSASDRKQIIRRVEMLLNRRHNAKPGWSKAVFAGVLLICLAAS
                     VVAAKMAPVITVDDPPAPVVAPAAPAAPAAPVVAAAPVAPRAPRAPSGQAPPTPPAPP
                     APAAEPAPASEPPLTPEQARRIAEQAQRASEQARQIAERMRPKQEEMQKLAEEMRKEV
                     EASIKPKTDEIRKLSEQIKQKVEASIKPHTREIAELAKKMAAEQAASKPNEEMIRKLE
                     QEMKRNEAEIERVVEKEVKALEEQIHAIELTIKPSEEQMRKFESKMQEMEKELEEQGR
                     ELERQFERDRPATPPPPAAPPARTTPPSAPPRAPAAPATPPAPPTPQAF"
     gene            complement(17717..18094)
                     /locus_tag="VLE22_13425"
     CDS             complement(17717..18094)
                     /locus_tag="VLE22_13425"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HSB15454.1"
                     /translation="MARKKSPTLTDAELRLMDVLWEKGAATVGEVAGALPMRPQLAYS
                     TVLTTLRILEEKGYVRHGRRGRAFVYRPVIDRTEVRRGAVRYILSRFFQDDPALLLVN
                     ILEHEKLDPEELQRLREMIEEGD"
     gene            18208..20787
                     /locus_tag="VLE22_13430"
     CDS             18208..20787
                     /locus_tag="VLE22_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15455.1"
                     /translation="MPHAAKALLLLCALCPAFAQKPEQPAKTRRTDLNLLGQTDVDAG
                     ESRRNENIQFNLVDNNALKELNVRLGTTATIIDEFRPSRSYFGSEFGNAPTPVLHLPA
                     AGRSPMHGNGYFSHLNSVFSARSFFQAGDVKPARENDYGLQLGAPLWRGSHLFLGGSQ
                     QKLRGSVNGNVLVPRSDERTPLATDPAVRSIIERYLAAYPTELPNRTDVNERALNTNA
                     PQVIDNDNAEARLDQQIATNDRLAIRYVFLSQTVDAFQLVKGQNPDTRTLAHTARATW
                     SRRWSAATSTDFSAGFDRVHSLLVPEPNAVGPMISISGLQTLGPLATIPIDRVQNLFR
                     YAGDAMSVRGNHTWTAGFGVLRRQLNGVETDVHRGFFSFTPDFGRDAITNFRMGIPTQ
                     HLRSIGDVHRGFRNWDLQFHAGDRWRATKNLTLTLGLRYNPVTTPVEVNRLNTIPYPC
                     DCNNLAPQFGLAWRLPRWWGIVRSGYGLHFGEIFPVTFQQVRLSPPGNYKIVVPHPSL
                     TDPLAPYYAGGDPNIVPTTYLLDDNLRTPYSHQYNFSWEPVLPGDWKLQLGYVGSRSH
                     KLLIMWYENRARLVDGMEPTTGNINLRRQNPHFAEIRRVLNGSRGYFDAARVSLVLPR
                     WRGLSIDAAYWFSKAIDLGSSYTNTAYDEDSRLSRSQTENLTHEDMKGPSPFDQPHAF
                     LWRAAYEIPKASGWPRRVRGAMGGWTISAVTLLKKGTPFTVASGSDAPGFGNVDGNGG
                     DRPNLLDPAVLGRTIGDPDTSRDLLPRSAFAYIRPEEQRGNLGRNTFRKGGIANVNAA
                     ISRTWPLRGDKQILFGAESINFFNTPQFAPPGFEMTSGNFGQITNTLNDGRTFRFLLR
                     FGF"
     gene            20807..>22663
                     /locus_tag="VLE22_13435"
     CDS             20807..>22663
                     /locus_tag="VLE22_13435"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSB15456.1"
                     /translation="MKRLLAISSLLIVPLGAQNQEFQALQGHFDRAISARFDNLFRGI
                     GSVKQWEERKQQTRSALTRMLWHDRQWPESPPRATLTRREEYRDYTVENLVLETAPKL
                     YSTSNLYLPRTGRKPFPVILYQCGHANKHYFTRHGAWFASHGIAVMVMDNIEMGEIEF
                     THHGVYSNAWFHWYSRGFSPLAVELLNAKRTLDYLCSRGDIDSRRIGATGRSGGGMTT
                     FFLAAIDERVKASAPVSGTLSTLGWVKQRLTFAHCDCQNPVNSYGLTYSEIGALTAPR
                     AQLLCNADADRGFPMDAFNEMAGKMNEIYRLYNAEGALRTSVTPGGHSDTEVIRLPVY
                     SFFLKELLGIDSPVTAEGPVDEPPKEQLVCFRDGIPTDDVLTRIDEELVPVYSYSLTP
                     MSAAARAKRIKELTGRLRNEVFRYFPSEKTPLEPAWSEQSTVQGRTIRKVTFPSFDGL
                     RVKAVYSLPSKASGSGKLPGLLIVDHRRGIPVWGNEQPLERNHWGDRAVLIVETIDRG
                     SRALEQNLRSFADDDPVHHMKRQAMVMGTTLESMQVYEILRSLEFLRSQPEVDASKIT
                     IAGKGETGVNGLYAALLDGNVERVELTSPPPSHRQGPHYLGILRYTDIPEVVA"
BASE COUNT         4194 a         7009 c         7377 g         4083 t
ORIGIN      
        1 tgccgcggtg aagcgcacgc cttgcctggc cagcccgtcg atagccggag tgcgaaccca
       61 ggggtacgct tccggatacg cactgatagt ccggtaggag tggtcatccg tgtagatgaa
      121 caggatgttg ggccgcttcg gcaggcccgc gctctgggat aggccgcgag ccgcaccgag
      181 gcccagaagg ccgagcatct ctcttcgact tcttcttacg atcggtggca tcagcacctt
      241 cctttccgat ccgccgcccg aagggcctcg gcgcggcgag cgtcaggaga gttcgatatt
      301 ccgcaggtcg attctggagt cgtcgaactc gccaagcccc aacgcacctg cgatctcgat
      361 gtgctcggtc tggcagttca ggcgcgtgct gaccttgtcc ggcttggaca gcgcgatggc
      421 cgccatcccc atctccgctc gcttcctgtc cagcgccttc cagccaattt tgtcgaccgc
      481 gaccggatcc gtagagaagt agattgtctt gtgttcccag atgtattggg gtctggcgga
      541 cggcccgccg tgatacacgc cctttacgcc atcaacgatg tgaagaaccg ccttttgccg
      601 gaagggctga agatcgacga tggccggaat gaagaccgaa gtacagtgca gcgtgctgtt
      661 ctggtggctg cgtgctacgt tattggccat cccgtatgcc atgttcttca aggccagagt
      721 gacgccggcc gactgatggt gcttcagcac ggggatgttg atcagcttgt tgatctcctt
      781 cgtcacgaac ttggccacgt acgatctgcg gaagtgccgg tcgttgggat tctgtccggg
      841 ctgtatgacc gggagctccg cgtaacagtc ggggtcgtag ccgtccatat cgagctgaat
      901 cggctcgtag gtctccgaag cccaggccca gcgcacgcct tccgggagcc acttgtcgta
      961 acccgcccgc acgataaacg agtggttgcg gtcgaatact acaatgtcgc gtggtttcaa
     1021 tcccgcctgc ctcagcccgt cgatgaccgc gtgcaggacc tccggcgcgg aaatcacgcg
     1081 cggcgcgccg ttagggttaa gtttgattcc aacaacgtcg ccgggttcga cgaacacgcg
     1141 ccaggcatcc acccaggttg gagcgccggt catctcgagc atcccgcgct cgatcatctg
     1201 tcgcaccggc ccttgctggt atttgtcgga atcgatgcac ccgggatggc tgaccgagac
     1261 aacccttccc gggaagggtc ctggaatgcc caacttgtcc ggcgcggggt tcggcgcccg
     1321 ccgggtctgg cctgcggtga tcaccgcagc ggaaagaaag tctcttcgtc ctgtcaacat
     1381 tggctgcctc ctgcctaccc cgacatccgg cccccataca attcgcccac gatgcgaagg
     1441 atctccttca tggatatgtg tgaggcctcg atcagggtgc cgtcgaagat gtgggtctcc
     1501 aagccgatct tgccttcgta gccgtcgcgt gccagcgcct ggaagatcgc cttccaatcc
     1561 agcttctggg agctggccgg catgataccc ttgcccttga tctgcacgtt gaggatgcgc
     1621 ttcttgggca ggatattgta accgtccgga aacgggacct cctgcgtacc aagggtgttt
     1681 tgcggatccc agttcagccc cacgtgccgg gacgggatgg cctcgagcag ggcggcgagc
     1741 tcagccgagc tggcgacgtt gcaggaggcc tcgttctcga ttgcaagcga gaccttctcc
     1801 ttttcggcga ccatcgccat ctcaccgatc acctcggcga tgcgcggaaa cagcgcgcgc
     1861 gggtcagcca cgcgcattcc cgtgaagacc cggatcttgt ccactcccag gatgtgcgcg
     1921 ttccgtatgg cggtgcgcag atcgtccatg cggcgatcga aacggacctt ttcggcttcc
     1981 agacgttggg cgcgcgcctc gggcctctcg ttgcggcggc ggaccggttc ggtacccggc
     2041 caggcgaatt tcaacagcga gctgttgaga aaggagacct tgaggtcgtg gccggctagt
     2101 gcagccgcag tgagcttgac ctcgtgttcg gggaggaagg cgtactcgtg gcgcgccccc
     2161 ggcacgcttc gcagctccac ccagcgcaac ccgtactcgc gggcgaagac gattgcgtca
     2221 tccggcgtct tgccgatctc gtccgtgatc gcggcgatgc gaagacgatc gatgggtttg
     2281 gccgcgtcca gtgagggcgc cagagcgatg ccggccgcca gaagctctcg tcgcgtcatt
     2341 cgacgatcca taatagcgcg gagcggaggt ggctacgcgg ccccgtctcg cacgcggaaa
     2401 agcgccttcg gtcctatcgg ctggagccgc ccgcctcgga aggaatatct tcggcacggc
     2461 tgtcaagttg tacccttgaa gagaacacat gtcgagcacg cggcgggatt tattgaggcg
     2521 cgccacagcc atggcggcgg cgccggctat cgcgcgacgc ctcgagaagc ggccgaatat
     2581 tctgtttgcc cttgctgacg attggggctg gccctccgcc tccattgccg gcgaccccgt
     2641 ggtcaaaacg cccgtgttcg accgggtggc gcgcagaggt gtgcggttca ccaacgcgtt
     2701 cgtgagctcg ccctcgtgta cgccctcgcg ggccgcgatg ctaaccggac agtggcactg
     2761 gcgtttggga gagggagcaa atctaggggg gacgctaccg gtccagtttc ccgtgtaccc
     2821 cgatatcctg gaggcggcag gctaccacgt aggcttcacc ggcaaaggtt gggcgccggg
     2881 cgacgaggct cccggcggca ggaagcggaa tccagccgga tcgcgctacc gggatttcac
     2941 cgcgtttctt tcggcacggc cgaaacgcgc cccattctgt ttttggttcg gcagcgccga
     3001 cccgcaccgg ccatacgacc gcgacagcgg ggccgccgcc ggtctccggt ggcaggatgt
     3061 acgtgtccca ccatatctgc cggaccacga aattgtgcgc cgcgacatcg ccgactacct
     3121 tctggaggta cagcgcttcg accgcgaaac cggtgagttg ctcgccatca tcgagagagc
     3181 cggcgaactc gataacacga tcgtggtgat ggctggagat aacggctggc cgttcccgcg
     3241 tgcgaaagcc accatctacg accccggcac gcatgttccg ctggccgtct cgtggccgtc
     3301 acgagtgagt ggcggccgcg aggtggaaga cttcgtcagc ttggccgacc tggcgcctac
     3361 gttcctggaa gctgcgggaa tccggccgcc atcgcagatg accgcccgcg gtctcctgcc
     3421 aatcctgaca tccaagcggg ctggcagagt ggacccccag cgcagccacg tattgacggg
     3481 catggaacgg catgttccct gtcgtggtca agccctcggc ggatacccga tgcgcgcaat
     3541 ccgcacgcgt gagtttcttt atatacgcaa cttcgagccc ggccgctggc ccgcggggga
     3601 tcccaacggt ctcgaagacc ccggcgctca gccgttctca ttcgacgaac ttgcgggtca
     3661 gacgttcaag gctctggctg atattgatgc cggtccaacg aaggcttacc tggtcaccca
     3721 ccggagcgat cctggggtcg agcctctgta cgcactcgcc gccggcaaac gtccggaacg
     3781 cgaactctat gatcttcgtg aggacccttt ccagatgagc aacgtagccg ccaaggcaag
     3841 ctacacggaa gtggtgaaag gcctcgacgc gcaactgatg gcggagttga aagccagcgg
     3901 cgacccccga gcgtacggtc gcggcgagga attcgaccga tacgtctggt atcaagggca
     3961 gcgcaccggc aagaagtgac ggtgcatggt tcaccaactg ggggctccac cccgagaccc
     4021 cacgaatcgt cttttccaac gagagagacc gcgcggtgcg gcttcggccg tgactgggtc
     4081 agggtcgggg agcttctgtc tcgatgtatc tacgaggaga aatcgcccag ccccggttgg
     4141 ggcagccgga cagtccaccg tatacggcct gaagcagcat cggaactggc cgcccactcg
     4201 gtctacgtgg agcagggaaa atccggcaag cggggagcgg gtaggcggcc gatgtttgag
     4261 cagatgaaac gcgatgccgc ccagcggaaa ttcgaccgtc ttttgatctg gaaagtgtct
     4321 catcttggtc gtgacatgcg ggaggtcatc gccacggttt acgagttggc cgacctcggt
     4381 gtcagtgttc tccccatcaa gtcccagacc ggccccatca actccacgat gggtaggttg
     4441 ttgtgggcaa tccaggcatg gtttgcggag atggagaacg acgaaagatc ggaggcgata
     4501 cgagccggcc acgcgcaagc tcgggccgca gggaaacaga ttgggcggcc gagaaaggtc
     4561 ttccgccgcg acctcgtcga ccagcaggag ctgccatttc gcggcttcat ggtgttcccc
     4621 agtatgtcct ttgttccgca aagacacttg tatacgggcg acttcgtgaa gtacactgcc
     4681 caacaacgag gtcgtcatgc aaaagtcttt tagccgacgt gatgcgctgt gctgggctag
     4741 cgcggttcca ttaacaacaa cttttctgcc tttatcccat catcgggcgt ttgcacagcc
     4801 agcaacgggc aagctgcaaa cccgcgttct gggccgcacg ggccgcgagg tgagtacgtt
     4861 cggtctgggt ggggtcacgt gcctaatgaa gttcggcgat tacgccacgg gactcatcgt
     4921 gaaggctgtc cgcgccggtg ttaccttcct ggataccgga aatggttacg gcgacagcca
     4981 gctaaattat ggcgccgcct tccgggagtt aaacgtggtc ccgggtcagc cgggctacca
     5041 ggcggccttg cggtcccgcc tcttcgtgag cacgaagctc gggagccgtt atgcgatcat
     5101 tcgcgacgcc tcggctccaa cggcggtggg atcccgcggg ggaggcgcca gagcgctaga
     5161 ccaactgaag ctctccatga cgcagatgtt cggcgacgga aaggggtctg tcgccgaggg
     5221 cgcctacgtc gatatgatgc acgtccacaa cctcaatacc gaggccgccg tcgacgcggt
     5281 attcgaggga atcgacaacc cgggcgataa atcgctgccc cgcgtgggta cgctggccct
     5341 cctgatcgac tatcgcgacg gcaccaactt gaccgggctc aatccggacc acaagaagtg
     5401 gattcgccat atcgccatga gctgccatga gaacccgacc tccgtcatgt acgccatgcg
     5461 gaaggaccaa aaaaacgttc tcgaagggct tatgtgtacg atcaacccaa acgatccaaa
     5521 ctacttctgc ttcgagacca atgcgctgcc ggtggcccag gccaagaacg tgggagtgat
     5581 cgcaatgaag gtcttctccg acggcgtgtt ttgggggaag ggcgagcatt ggggcggcga
     5641 gtgggttaaa acgatcggcc tgccggataa ggtatcctat caggatttca tcggatacac
     5701 gctttcgtcg ccgggtgtgc acgccgcggt catcgggatc ggcgaggtcg ataagaacaa
     5761 cgatccacag cgcgatcagt tcgtcgccaa cttggcggca tttcagaccg cgcgcgctct
     5821 aaccttgcgg gaacgaaagg acatcatgga gcgggtcgcg ggcctgcatg gcacgcatac
     5881 gaacttctac cagaggccgg gaagcgggtt gcagccaccg cagaacgtga gcgttgcccg
     5941 gccgcaaaac gggccggtcg aggtgtcgtg ggacacggca tacgccgctg acaatgcact
     6001 ggtaagatat gagatcttcc gccgccacga cctggtcgca cgggtgcctt tcgccccgca
     6061 gatcaccgag cagccgttcc ggttcgtaga cgacggcgtt aagggcagct tccccggcgg
     6121 gctctggtac cgcgtccggg tagtggactc ggaaggggcc acggccgatt cgaataccat
     6181 tctggctccc caggcacagg ggcgcgccgg atagcggccg ccacccgcca ccatgtcgcc
     6241 gtaccccctg acgacgcaat agacctccta ctcccgatcg tgatggtgag taatgttggt
     6301 ctccgtggcc aggcggcggg ccgcgagcga agggccgccc tcttcccacg tataggccac
     6361 cagacccagt ccgaaattcc agatgtgttc ctgcgagggg cggtagcgct ctcctttcac
     6421 ctcgatttcg gccttcgatg atcgcccgag gagctaggtt gaatgcggga gcaggccggt
     6481 gtatgctacc gactaatcga taaaccccca aaggagagag cccaatgagg ccgttagcag
     6541 ttttcttcgc gttcatagcg atcgggctgg cggcggacaa gaaggcggtg agccctccgg
     6601 gtgtcacccc cgtcgggcca tacagtccgg gcgtcatggt cggcgacttc ctttacgtat
     6661 ccgggcaagg cgcgcctggc ccggatggaa agatgcccga cacactggaa gggcaagttc
     6721 gccaggcgtt gaacaacgtc aaggcggtgg tagaggcggc ggggctgacc atggagcacg
     6781 tcgtttatac tcacctctac ctgaccgaca tgagcaccta cgagcaggcc gacaaggtct
     6841 gggcggagtt cttccccaag gcgcctccgg cgcgcgcggt tgtaggcgtc tacaagttgc
     6901 cggggggcac gccggtggag gtcaacgcgg tggctttccg ggacttggaa cgcaagaagg
     6961 tgtttacccc ggcgggtttt cccgcgcatc ctggcgtgtc gccaagtgtg atggccgggc
     7021 agaggttctt cttctcctct gcattgggga tggacatggc ctcgggcaag gttcccacga
     7081 accccgacga acaggtccag gtggctttcg aaaatctgaa gcgcatgctc gacgccgcgg
     7141 ggctggattt ccgacacgtt gtgatggtca atccctacct ggcggccggg tcgtcgccgg
     7201 ccatgaacaa gatctacgct tcgcatttcg aatttggcaa cacgccgggg cgggcgacta
     7261 ttcgggtcac cagtcttccc cagggcaaca ccatcgcgtt cacgggagtg gccatcgctg
     7321 acctttccaa acggctggcc gtgaggccga agaacatggc gccgagcccc accgcgagcc
     7381 cgtgcgtgct ggcggacgac acgctctact gctcggctaa gtccggattc atccccggcc
     7441 cgaacgaggg catctacagc cccaccgtgg agggccagtt gcggcaaacc atgcggaact
     7501 tgctggacgg cctcgaagag gcggggatga acttctccaa cgtcgtagcc accaacgtct
     7561 acctgggtga catcgacgaa tttgccaaga tgaaccgggt gtatgcccag tactttacgg
     7621 gtgttgcgcc cacccgcact accgtcgcgc agacagcgcc aagagaagtt gcacagccgc
     7681 ccgctaacgc caatcgcccg ccgaacccgg atacctttcc cgggcgagaa cagatttcac
     7741 tcattgccgt gaaatagatt caactgcgtc gcggaaacgc ggcgggagat acatcaatat
     7801 ggccacagat acttcttcca ccagcagacg tgctcttttc agacagatca gggcgctccc
     7861 tttgtttgga tactttgccg cccaggacct ttgggcgatg gcccggaagg ccgcgggacg
     7921 cgcgtcatcc gctgacatct atgcgcgcat cggcgtcaca ccgatcatca acgcccgggg
     7981 cacgtggact tacatcagcg gctcgctcga acttcccgaa gtcaaggccg ccaaacaggc
     8041 ggcggccgag catttcgtgg acatgtggga gttgcagaag ggcgtcagca agcggcttgc
     8101 ggaactctcc ggcgccgaag ccgggatgat tacggcgggc gccgcgtccg ccttgggaac
     8161 ggccaccgcg gcctgcatgg cggggaccga ccccgccaag atctggcaac tgccggacca
     8221 caccgggatg aaatacgagg tgatcatgtt cggcgggcgc ctctcgttcg acagcgccat
     8281 ccgggccgcc ggcggggtgc tggtgatggc ctatagcccc gaagagctgc aggccaagat
     8341 caacgagaac accgtgatgg tcttcaccac tgctcgcggg gagcgcctgg aaaaagggct
     8401 ggtcatcacc aagaaggccg gcgtgccgat gttggtggac tgggcggcgg gcatccctcc
     8461 catcgagaac ctgtcgcttc cggccaagat gggggcggac ctgtacacct tcagtggcgg
     8521 taaggggttg tgcgggccgc agtgcaccgg cctgctgctc gggcgcaaag acctcatcga
     8581 ggcggcgctg gcgaacagtt gcccctggga gggagccgtg tgccgcgccc acaaggtcgg
     8641 caaagaagag atcatggggt gcctggcggc catcgaagcc tggaagaaga gggacctggc
     8701 gaaactggac cgcgagtggg gcgcacgtgt ccggcgaatc gttcaattgg tggagacggt
     8761 gcccggcgtg aagactgaga ttaccactcc ggcgggtgga aaccggtatc ccaccctgtc
     8821 ggtgagttgg gacgaggccg ccttccagat gacggtactc gaatgcgcca aagagttgcg
     8881 cgacggccgc ccacgcatcg aagtgctgac cccgaccaat cccagtttgg ttccggtggt
     8941 gcgggagggt gatcccaatg cgccccgacg gcgaggcgaa gacgcccccc gattgcggat
     9001 cgtgtcgctg accttacagc ccggtgagga gttgttagtg ggccgccgcc tgcgcgaggt
     9061 gttgtcagaa gcgcgcaaga aggcccaagg ctaaggggca tggacgcaca tcgagattca
     9121 aacgcccttg ctagggcggt gatgggctaa aacccacgga cggggccggc gcgcgggcga
     9181 ccgctacagg tttcgtctgt acaatctcat catgcgacag gttctgctgc tctccctggt
     9241 tctcttgctg tccccaccgg tctcgcgtgc ggcgaagacc ctcgacatgt atgcgatcga
     9301 caccgagggc gggaaggcgc tgctgatcat tgcgccttcc ggccaatcga tgctcatcga
     9361 caccggctac cccggttata acggccgtga cacgaaccgc atcgtggcag ccgcccaggc
     9421 ggcgggagtc aagaagctcg atgtcctggt taccactcac tacgatatgg accatgtgta
     9481 taacgccccg tcggtggtgg ccaagattcc cgtggcgttg tttgtcgatc acgggcaggc
     9541 cgttaagcag ggcgcatcta ccgccaaagc cttcgccgcg tacttggagt tgactgccaa
     9601 ggccaggcgc atgattgcca agcccggaga cacggtcccg ttcaagggcg tagatgtcct
     9661 ggtggtggcg tccaacggag aagcgctcaa gactccgctc aagggcgcgg gcgccacgaa
     9721 tgcggcctgc gcaagcacgc cgcagaagca gactgacaag acggagaacg gcttctcact
     9781 cggcctgctg ttcacttacg gcaagttccg catggtggac ttgggcgacc tctcctggaa
     9841 cagggaactc gagatgatgt gcccgaacaa tcccatcggt acggtcgatc tgttcatggt
     9901 cagctaccac ggaggcgacc cggcgaactc gccggcgttg atccacgggc tcaggccccg
     9961 ggtgacgatc atggacaacg gagtcaagaa gatggggacg gccagtgtgc tgaaaaccat
    10021 ccaatcatcg cccgggctgg aagctgctta tcagttgcac tggtcgatca acgcgcccaa
    10081 cgacaaccca cccgacgagt tcatcgccaa cctccagaac tcgaccgacg gcaattggat
    10141 caaggtgtcg gcgcggaagg acggcgcctt cacggtcact aatgcccgca cgggggcctc
    10201 ccggacgttc cgcaagtaag gcgaggcagg tgaagccact tgccagaaac aattcgtgcg
    10261 ttctcgcaga ttacgttggc gtccggcagc agccgctcct atgcgtacgt cagatgccgc
    10321 tggatcggat ccagaagctc tcagtccact tgccgccggc gggcacggtt tgcaggccag
    10381 accacttgcc ttcgtgtgcc aggttcacgc cgctgatgat cgtagccagc ggttcaaaac
    10441 agatgaacgg ccgcggctgt ccatcgggac ccgagggcag ccagacggtc gccacggtga
    10501 acttcggccc gaacagagtc tccacggtct taccgcccgc ttcgatggtg aagtgcgcac
    10561 gcccgtccgc atcccgctcc aggtccgtga agccgtcgtc cagcttctgg cctttgagca
    10621 gcagcggatt cggcacgtcc atcggccgca tctctccggt cggaatgttg ttttcgcccg
    10681 gaatcacgtg gatgcgagcc gggatgcgcg ccatccactc atcgcgcgga atatccggca
    10741 cctgaaaaaa cgggtgaaac cccagaacca gcggcatgct ctcggtgctc agattcgtga
    10801 cggtgattct cacttccagc acgccatcgg ccaggccgta ggtcatctcg tactcgtggg
    10861 cgaacggcca ctgtgccatc aggtcaggat acttccagaa ctccaggcgg ctggcgacgt
    10921 gcgccgagtg gccgtccgac gccacctccg tgacgtgcca gagcggcgaa ttgatcagca
    10981 agccgtggcc cggcaagttg ccgcgtacgt tgcccagatc catgttgaag tggtagtgct
    11041 tcccattagc ccagaagccc ggctcgttca gtaggtcggc ccagggcgcc aggaagggaa
    11101 tgccgctcag tttcggccgc tccttgaaat cgccgacgtc ggtggatgca aagtacagaa
    11161 tgttcttgcc gtgtaccttc atctcggtga cccggttgcc gatcgaggga accaccgtca
    11221 cctccacgcc gtgtgccgcg tccgtcaggc gcacgatgtc gacgccgtga tccgtcgtct
    11281 gttcggcctt gtagttctgg gcaaaggaga ccagcggcgg caacagcatc accgcggcaa
    11341 agcaatcggc agcactcatt ctcatagaca gtccctcatt tcgcgtcccg ccgccgtact
    11401 tgcggccgtt gaggacctga ggtcccattt tagaggcgac tcgtttttct tgtcgtccgc
    11461 ccgttcttca cgttcatcgt tgcgcgtact tgggcacgtc gaacgtgtag gagccgtcag
    11521 gattcactgg ctcggcgcag gcccaccgca ccggccgacg gatcccggct ttggtctcca
    11581 cctcggcgcg cagagtcatc ttctgagcct caaagccttt cggcaggatg aaagagcact
    11641 gccttagttt ctctccgtag gggtgcatcg agggtccgcc ccccgacctc agcaggtagg
    11701 tgcactttca gagcgcttta gcgccgctca caaacgggcg agggcggcga tgtcccagga
    11761 ggggtccggg aggacctcga cgttcggggc ggagccgtag gctgaccgga cgacggcggg
    11821 ttccatgcgc ttgccggcgg cgtccaccgt accagcgagc cagaccttgc gcggggcaag
    11881 agaccccgca acttgcggaa gatccgcccg cgaaagcgct ccgtagacta ggttggccag
    11941 cgggtattcg aagtcctccg tctccacaac gctgcgccag gagacgaggc ccccagcaag
    12001 gtacacgccg gcgatgccgg gatcgagcgc ggcggcgaac aacgccggaa cagtcatcgt
    12061 gccccgcgcc gccagcacga tccggcgccc gcgggcggca ttgtgcgcgc gcagagcggc
    12121 ggcgaaggcg agcaggtccg tgactctctg gccgagcagc ggacgcccga gcatcagcga
    12181 cgaccaggcg tagttctcct cctcctggtg ccatcgctgg tagcccgcgg cgccgggact
    12241 gacctccggc acaaggtctc ccgtacctcg gacatcggcg gcacagacga cgaggccggc
    12301 ctcggccagg gagggataca ggtcgccctc ccgccagcgg ctattccggc cctgcggctc
    12361 ggcgatgatc agcacaggct tgcctgtttc cgccttgcgc ggcaggaaga gccacgcggg
    12421 agcgaacacg cgcgaggtgg agggcacttc aatcgcttcc acatcgaccg gaccggcgga
    12481 cacgcgcccc actaccgatg cgcgcggggc ggcagcgggc ttctcgacgc gcagcagttc
    12541 gtcgatgccg gcggggcggg cgggcgtctg aattcgctca gcctgcttcc ggttcaggct
    12601 gaacggcgtc tcgctgtcga agtcgcggac gacgttgctg gtcggaccgg cccagagcgt
    12661 ctcgtctttc tcgacgttga ccggcggttc ttcgcggacg ggttccggct tgtcctggag
    12721 ccaccggcgg aaccagttgt aggtcttcaa ccgcaatgcg tagccgaagt tgtgcggcat
    12781 cggcgtgtcg ccccactcga ggcggtcacc atggccgaga acctcgtaca cctttttcag
    12841 cttcttgtac tcctcccagc cgctcgagag gtagttgggc gagtaagtgc caaagtagtc
    12901 cctggcgctg acgagcacga gcagtggctt gggagcaagt gggtacaacg tatcccagcg
    12961 gtcgaagccg gctgggccgc catcaaggaa gttctgctcg gcgtcgtcgg tggagccggg
    13021 cgggaggaaa ccggcgcacg cgaggttctc cgtgttgccg ctcgagacta ccgcggcggc
    13081 gaggcggtca tccacggcgg ccagtagcat ggtgagtgtg gcgccgccgg actggccggt
    13141 ggaggcgagg cgcttcggat cgaccatcgg gtgctcggct aggtagtcga ggctgcgcac
    13201 ggcgtcccag gtctggaggc gagtggaggt gtcgccggcg aggatcatct ggaagcctgg
    13261 cttggtgtgt tcctcgtcgg ctgaggaaag gcgggtgagc acaccgccgg ctctcggata
    13321 gtaggtccgc tctccctgtc ccatcggatc gaaagcgagc acggcgaagc cgagacgcgc
    13381 cagtccctgg caccccttct ggtaggaatc gcccgccttg ccattgagcg tgtgacccat
    13441 ctggaagagc acaccggggt acggcggctt gccctccgta ggcagataga aattcgcggc
    13501 gatgtggaag ccggggcggc tctcgtaaac gatcttctcg aggcggtaac cgggacggtc
    13561 gaacgacccg acgacacggg cgttgagcgg ggtccgttcg ggacggccgc cggtgagctt
    13621 ccagaacgtt tcagcaaccc agcgttggcg gaggcggatc gcctcgacgg tggtaagctt
    13681 cccgatctcg cggttgcgtg cttggtaagc gcgcatggcc agcgcgtaga gatagtcggg
    13741 cagacagcgc gaataacgcc ggtaagcaat gcctgggtag ccgtcctgtg cgagcactcg
    13801 tggcgcggcg aaggccgcgg tggtggagaa gaagtcgcgt ctggtcattc gctccacgat
    13861 atcactggga acgcaacgcc tggatttgtg cgccgatctc gtcgaggcgg cggagcagtt
    13921 cgtcgtggcg gcgctcctct cggggcggcg ctttgccctc gatgaaaaag gcgccgccgg
    13981 gttcgagcac gcagcgctcc acctcattca gacgcgagaa gccttggcgg tgcagcacgg
    14041 ttagcaactc ggactcggtc aatagctccc tggagagtgc gtctcttctc accttgccat
    14101 gttcgatgag cacggccggc ctgcccgcga ggatctggtc gaggcggcgg tgccggaaca
    14161 gaaagcgaac gacgacatag ttgatcccga gtagagcaaa ggcgcctagc aggccgccgc
    14221 tcaccgagtt gtcgtttccg atgatcgcat tctgcaccgt gttggacagc gagagcagca
    14281 ccacgaggtc aaacgggttg agctgcgcga gttcccgctt gccgaagatt cgaaggaagg
    14341 cgacgaggac gaggtaaacg attaccggcc gcagcacttt ctccgcggcc gggagcccga
    14401 gctgaaacat gtcctgcagc aggccggtca tcttcggtat ctcctttcaa ggcttgacga
    14461 gcgagccatc catgccgcgg actgtggtcc agttgtcgcg gccgggaggc ggttcgagcg
    14521 ggtacttcgc ggccatcgcc tcgttgatgt cgataccgag gccggggcca tcactgccgt
    14581 aaaggtaacc gcccttgagc tgcgccgtgc ccggcatcat ctcatgaacg agcggtggga
    14641 agtggttctc ttcctggatg ccgaagctgg atatcgacag gtcgacgtga tagctcgcga
    14701 gctggttgat ggggtcgttg tcgccgcctt cctgaaacgc cgtgcgcacg ccgaaggttt
    14761 cgcagagggc tgcgatcttc ttcgccgaag tgatgccgcc ggtctgcgag acgcggcagc
    14821 gaataaagtc gatgaggcgc tccgagatca gcgggaccca ctcgtgtggg ctggtgaaca
    14881 gctcacccat cgccatcggc gttgtggtga cctggcgaac ctggcggaac catgcaatct
    14941 gctcgggcgg gaagaggtct tcgaggaaga agaggcggta cggttcgagc cgcttggcaa
    15001 gctcaacgcc catgatggcg gtcaggtgct cgtggacgtc gtggagcagc ttcacgtcct
    15061 tgccgagctt ggcgcgaagg tgctcgaaca gtttgggaat cgtgtcgacg tagagctcgt
    15121 cgtcgaaagc gggcccggcg tagccgccgg cggggcggct cccctgcccc ggttcgatca
    15181 tccctccgcc gccatagccg cccatctgcg cgcggacgtg acgatagccc tcgtccatgt
    15241 acttgcggac attctccgtg acttcgttca tgtcgcggcc gtcggcgtgc gcgtacagcg
    15301 cgaccgcgtc acgcgccttg ccgccgagca gttcgtagac tggcatgcct gcgcgcttgc
    15361 ccttgatgtc ccaaagcgcc atgtcgagcg cgctgagcac gttgttctgg atcgcgctgt
    15421 tgcgccagta cgagcgaacg ttggtgctct gccagaggtc ctcgatcctg tcggcgctct
    15481 tgccgatcca gaacggcccg aggtgtttct cgatggcggt ggccacggtc agcgcctggt
    15541 tcacgttgct ggcggagccg aggccgtaaa gcccgggctc gctggtctcg atcttcagga
    15601 acacccactg gtagcgcccg ccggcgctcg tgggaatcgc cttcaccgag cggattgtaa
    15661 gcggtggaag acctcgcgtg cgctgggcgt agagcgaggc ggacaacagc ggcagggaca
    15721 agaagtcgcg acgaagcatg gttcctcccg cagttgatta tatggaggaa tcatgcttcg
    15781 tcaagaaaac cggggactct ccggggtgat tttgtaggtt cagtcgtatt ggaacggaac
    15841 gcaggcgtgt ccccaggtct ttgggaatca gaaggcctgc ggagtgggcg gggccggagg
    15901 ggtggcaggc gctgccgggg cccgcggagg ggcggacggc ggcgttgtgc gggccggcgg
    15961 cgcggctgga ggtggcggag tggcggggcg gtcacgttca aactggcgct cgagctcgcg
    16021 tccctgctcc tccagttcct tctccatctc ctgcattttg ctctcaaact tgcgcatctg
    16081 ttcctcggac ggcttgatgg tcagctcgat ggcgtggatc tgctcctcca gggctttcac
    16141 ttccttctcc accacgcgtt cgatctcggc ttcgttgcgc ttcatttcct gctcgagctt
    16201 gcggatcatt tcctcattcg gcttcgaggc tgcttgttcc gccgccatct tcttcgccag
    16261 ctccgcgatc tcacgggtgt gcggcttgat cgaagcctcc accttctgct tgatctgctc
    16321 ggacagcttg cggatttcgt ccgtcttcgg cttgatgctc gcctcgactt ccttgcgcat
    16381 ctcttcggcc agcttctgca tctcctcctg cttcgggcgc atgcgctcgg cgatctggcg
    16441 ggcctgctcg gaggcgcgct gggcctgctc ggcgatgcgc cgggcctgtt cgggcgtaag
    16501 gggcggctcg gaggcagggg cgggctccgc cgcgggagcg gggggagccg gcggcgtggg
    16561 cggcgcctga cccgagggag cccgaggcgc gcgaggagcg accggtgcgg cggcaaccac
    16621 cggagcggcg ggggctgcgg gggcggcagg cgccacgacg ggcgccggcg ggtcgtcgac
    16681 ggtgatgacg ggcgccatct tcgcggcaac cacggaagcc gccagacaaa tgagcaacac
    16741 tccggcgaag accgccttcg accagccggg cttcgcattg tgtcttcgat ttagtaacat
    16801 ttcgactctc ctgatgattt gcttgcggtc gctcgcggaa cccagggcgg gagccgggtg
    16861 gggggtgagc gacgcgtatt cagccagccg gaccagacac gcagcataag gacgcgcgga
    16921 gccggaaacc gcgacgaccc agtcgtcgca ggcgatttcg cgttcgaggt tgaggcggtt
    16981 gccgatccaa cggacaccgg ggtggaagaa caaggccgtc tcggcgatcc gctggaagag
    17041 attggcccaa tcatcgcaac gccggatatg tgcgagctcg tggaggcaga tgcgaaactt
    17101 ttcttccgcg gtcaggcgcg gcaccaaaga ctccggaaac aggatggctg gccgcgtcag
    17161 gccggccagc atcggcaggg gtgcctcgga ggaaccgcgg acatcgaagg cgcggcgcgt
    17221 gaagcaggcg gcgaaccact tcgaccaacg tcccgtgtcg ggatcgtcaa gcggcttcga
    17281 gttgttcttg aggcgggcca tgcccagata gctgcgcagc agcctcacgc ccagcaacag
    17341 cgcgacgaca acccagccgg caagcagcaa cacggcggca gcagcgggaa gcgaaagatc
    17401 gggaggactc cagacgctcg cggatgcgcc ttccggcggg gccgacgatg acgagaccag
    17461 ctcgggggtg atgcgcgccg gcggcgcctc aatggcaacg gccggtgcgg acggagcggg
    17521 ccacatcgac acggcaacat gaaccgccgg caaagacagg accgcaacaa gacaggcgta
    17581 ccaggccgcg taacgcatcg ccgcgctgca cggcaggaaa cggagcagca gccataacag
    17641 cgccgtcagc agaatgccct gccagagccc gttgagagtt gcctcaatca gaagtccggc
    17701 caacgtatcg aacatttcag tcgccttcct cgatcatttc ccgcaagcgc tgcagctcct
    17761 cgggatcgag cttctcgtgc tcaaggatat tgacgagcag gagtgccgga tcgtcctgaa
    17821 agaaacggct caaaatgtag cggacggccc cgcgccggac ttcagtgcgg tcgatgaccg
    17881 ggcggtagac gaaggcgcgg ccgcggcggc catggcgtac gtagcccttc tcctcgagga
    17941 ttcggagggt ggtcagtact gtgctgtagg cgagctgggg tcgcataggc agcgccccgg
    18001 cgacctcgcc gacggtggca gcgcccttct cccaaagcac gtccatcagg cggagctcgg
    18061 cgtcggtgag tgtcggcgat ttctttctgg ccatgggcga ataccgctct cagcaactaa
    18121 ggaattaata gttccaacct aacaatacgg caattcctgc tgcttgtcaa ctaaataatt
    18181 aatagttcgc acggggtaga atgagcattg cctcacgccg ccaaagccct tcttttgttg
    18241 tgcgccctct gcccggcgtt cgcgcagaag ccggagcagc ccgcaaaaac ccggcggacg
    18301 gacctcaacc tgctcggcca gaccgacgtc gacgccggcg agagccgccg caatgagaac
    18361 atccagttca acctcgtaga taacaacgca ttgaaggagc tgaatgttcg attgggaacg
    18421 acggcgacga tcatcgacga gttccggccg tcgcgaagct acttcgggtc ggagttcggc
    18481 aacgcgccga cgcccgtcct gcacctgccc gcggcgggac gttccccgat gcacggaaac
    18541 gggtactttt cgcacctgaa cagcgtgttc agcgcgaggt ccttcttcca ggcgggcgac
    18601 gtgaagccgg cgcgggagaa cgactatggg cttcagctcg gggcgccgct ctggcggggc
    18661 tcgcacctgt ttctcggcgg gagccagcag aagctgcgag ggagcgtgaa cggcaatgtg
    18721 ctggtcccgc gctcggacga gaggacgccg ctggcgacgg acccggcggt gcgaagcatc
    18781 atcgagcgat atctcgccgc ctaccccacg gaactgccga accgcacgga cgtcaacgag
    18841 cgggcactga acacgaacgc cccgcaggtg atcgataacg acaacgccga ggcgcgactc
    18901 gaccagcaga tcgcgacgaa cgacaggctg gcgatccgct acgtgttcct ttcgcagacc
    18961 gtcgatgcat tccagcttgt gaagggccaa aatccggata cgcggacgct cgcgcacacc
    19021 gcccgtgcca cctggagccg gcgctggagc gctgcgacca gcacggactt ttcggctggg
    19081 ttcgaccgcg ttcactcgct ccttgtgccg gagccgaatg ccgtggggcc gatgatctcg
    19141 atcagcgggc tccaaacgct ggggccgttg gcgacgattc cgatcgaccg cgtgcagaac
    19201 ctgttccgct acgcgggcga cgcgatgtcg gtgcgcggca accatacgtg gacggcaggg
    19261 ttcggagtgc tgcggaggca actcaacggc gtcgagaccg acgtacaccg gggcttcttc
    19321 tcgttcactc cggacttcgg gcgggacgcc atcacgaact tccggatggg catcccgacg
    19381 cagcatttgc gctcgatcgg cgacgtccac agggggttcc ggaactggga tctacagttc
    19441 cacgcgggcg accgctggcg ggcgacgaag aatctcacgc tgacgcttgg actccgctac
    19501 aacccggtga ccactccggt cgaggtgaat cgcctgaaca cgattcccta cccctgcgac
    19561 tgtaataacc tggcgcctca gttcgggctg gcgtggcggc tgccgcgatg gtggggtata
    19621 gtgcggtccg gttacgggct gcacttcgga gagattttcc cggtgacgtt tcagcaggtg
    19681 cggctcagtc cgcccggtaa ctacaagatc gtggttccgc acccgagcct gacggatccg
    19741 cttgcgccct actacgcggg cggcgatccg aacatcgtgc cgacgacgta ccttctcgac
    19801 gacaatctgc ggacgccgta ttcgcaccag tacaacttca gttgggagcc ggtgctgccg
    19861 ggcgattgga agctccagct cggatacgtg ggaagccgtt cgcacaagct gctgatcatg
    19921 tggtacgaga accgggcgcg gctggtggac ggcatggagc cgaccacggg caacatcaac
    19981 ctgcggcggc agaatccgca cttcgcggag atacgcaggg tgctgaacgg ctcgcgcggg
    20041 tacttcgacg ccgcccgcgt gtcgctggta ctgccgcgct ggcgtggtct gtcgatcgac
    20101 gcggcctact ggttcagcaa ggcgatcgac ctgggaagca gctacacgaa cacggcgtac
    20161 gacgaagact cccggttgtc ccgcagccag accgagaatc tgacgcacga ggacatgaag
    20221 gggcccagcc ctttcgacca gccccacgcc ttcctgtggc gggccgccta tgagatcccg
    20281 aaagcttccg ggtggccgcg cagggtgcgg ggtgcgatgg ggggctggac gatctcggcg
    20341 gtgacattgc tgaagaaggg caccccgttc acggtggctt cagggagcga cgcgccgggc
    20401 ttcggcaacg tagacgggaa cggcggagac cgcccgaacc tgctcgaccc ggcggtgctg
    20461 gggaggacga tcggagaccc ggacacgtca cgcgacctgc tgccgcgatc ggcgttcgct
    20521 tacatccggc cggaagaaca gcgaggcaac ctggggcgga atacgttccg gaagggtggc
    20581 atcgccaatg tcaacgcggc gatttcgcgg acgtggccgc tccgcggcga caagcagatt
    20641 ctgttcggag ccgagtcgat caacttcttc aacacgccgc agttcgcacc tccgggtttc
    20701 gaaatgacct cggggaattt cggccagatc accaacacgt taaacgacgg ccgtacgttc
    20761 cggtttctgt tgcgtttcgg gttttgaggt tactggggtt tatgacatga agagattact
    20821 cgccatttcc agcttactga ttgtgccctt gggagcgcag aaccaggagt ttcaggctct
    20881 tcagggccat ttcgaccgcg cgatcagcgc gcggttcgac aacctgttcc ggggaatcgg
    20941 gagcgtgaaa cagtgggagg agcgcaaaca gcagacccgt agcgccctca ccagaatgct
    21001 gtggcacgac cggcagtggc ccgaaagccc gccgcgcgcg accctcaccc gccgcgaaga
    21061 ataccgcgac tacacggtcg agaacttggt tctcgagacc gcccccaaac tctactcgac
    21121 gtccaacctt tacttgcctc gaacgggccg gaagccgttc ccggtcatcc tctaccagtg
    21181 cggccacgcg aataagcact acttcacccg gcacggtgcg tggttcgcca gccatggcat
    21241 cgcggtgatg gtcatggaca acatcgagat gggcgagatt gagttcaccc accatggcgt
    21301 ctattccaat gcctggttcc actggtacag ccggggcttc tcgccgctgg cggtcgaact
    21361 actgaacgcg aagcggacgc tcgactacct gtgcagccgc ggcgacatcg acagccgccg
    21421 gatcggcgcc accggcaggt cgggcggggg aatgacgaca ttcttcctgg cggctatcga
    21481 cgaaagggtg aaggcttccg cgcctgtctc cgggacttta tcgacgctgg gctgggtgaa
    21541 gcagcggctt accttcgcac actgcgactg ccagaacccg gtgaacagct acggcctcac
    21601 ctattccgag atcggcgccc tgacagcgcc gagggcgcag cttctgtgca acgccgatgc
    21661 cgaccggggt tttccgatgg atgccttcaa cgagatggcc ggaaagatga acgagatcta
    21721 ccggctgtac aacgccgagg gagcgctccg gacatcggtg acccccggag ggcactccga
    21781 tacggaggtg atccgattac cggtctactc gttcttcctc aaagagctcc tcggtatcga
    21841 cagcccggtg accgccgagg gcccggtgga tgagccgccc aaagaacaac tggtctgttt
    21901 ccgggacggg attccgaccg acgacgtgct cacccggatc gacgaggagc tggtcccggt
    21961 ctattcctac tccctgaccc cgatgtcggc cgcggcccga gcgaaacgaa tcaaggagct
    22021 caccggccgg ttgcggaatg aagtgttccg ctacttcccg tcggagaaaa cgccgctcga
    22081 acccgcgtgg agcgagcaga gtaccgtcca gggccggacg atccggaagg tgacgttccc
    22141 gtcgtttgac ggcctgcgcg tcaaggcggt gtattccctt ccctcgaaag cgtctgggag
    22201 cggtaagctg ccgggcttgc tgatcgtgga ccatcgcaga gggattccgg tgtggggcaa
    22261 cgagcaaccg ctcgagcgca accactgggg agaccgggcg gtgctgattg tcgagaccat
    22321 cgaccgggga agccgtgccc tcgagcagaa cctccggagc ttcgccgatg acgacccggt
    22381 gcaccacatg aagcgccagg cgatggtgat gggcacgacg ctcgaatcga tgcaggttta
    22441 cgagattctc cggtcgctcg agtttctgag gtcccaaccg gaagtggatg cgtcgaagat
    22501 cacgattgcc ggcaagggag agaccggagt gaacggattg tacgcggcac tgctcgatgg
    22561 aaacgtggag cgggtggaac tgacgtcacc gccgccgtcc cacaggcagg ggccgcatta
    22621 tcttggaatc ctgcgctaca cggacattcc ggaggtggtg gcg
//
LOCUS       DATIFQ010000286         3883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_669149, whole genome shotgun sequence.
ACCESSION   DATIFQ010000286 DATIFQ010000000
VERSION     DATIFQ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669149"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VLE22_13440"
     CDS             <1..432
                     /locus_tag="VLE22_13440"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15457.1"
                     /translation="RVDLVENRYVGMKSRGVYETPGGTLLHAAHRAVESITMDREAMH
                     LRDSLMPRYAELVYYGYWFAPERAMLQAMVDESQKNVTGTARIRLYKGNCDIVGRKSA
                     VSLYNPELATFEADAVYNQKDAEGFIRLNALRLRVEARTRK"
     gene            521..1942
                     /gene="argH"
                     /locus_tag="VLE22_13445"
     CDS             521..1942
                     /gene="argH"
                     /locus_tag="VLE22_13445"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSB15458.1"
                     /translation="MTRRTAKKKTNSKEQPGRKPWGGRFTEATNRLVEKYTASIPYDW
                     RLYPYDIAGSTAHAAMLAKTGIITRKEAARIIRGLDEIQKEITGGSFPFSIELEDIHM
                     NIENRLIRMIGPVGGKLHTARSRNDQVALDVRMYLRDEIAELHELAGALQKVLVGLAD
                     AHRDAVLPGYTHLQRAQPVLLGHHLLAYYEMFERDRDRLKDCFRRVNILPLGAGALAG
                     TVLPIDRRFVARQLGFSGLCENSMDAVSDRDFAIEFAADCAQIMMHLSRLCEELVIWS
                     SAEFGFITLPDAFTTGSSIMPQKKNPDVAELARGKTGRVYGGLMALLTIMKGLPLAYN
                     RDMQEDKEPLFDAADTVAHSLAVVAEMLKGVVFHKEAMRKAAGDGFITATDLADYLVR
                     KGMPFRRAHEIVGKAVLRAMELGCGLQDMPLAEYRKLSRQINRDVFQAISLESSVGRR
                     RSYGGTAPGNLDKRLEYLKKKMK"
     gene            1939..3000
                     /locus_tag="VLE22_13450"
     CDS             1939..3000
                     /locus_tag="VLE22_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15459.1"
                     /translation="MKTRYRWCVAVCLLALAAASACGRKTNPLVPDSPRPEAIKVIKA
                     VSRGGVVYLTWQMPTRNIEGKSYDPSGIRKIFIDRAEPSEDHKKARYKRSAEIDVADM
                     PPSLLRDNVATWSDGQVRYGQTYAYRLRAVSAHGISPWSEPVLIVPQASLAAPKGLTA
                     TKGDSSVGLTWDPVRTLLDGGPAEGFIGYNVYRGTDKGIHDEAPLNREPLTATSFKDA
                     TAVNEHTYYYIVRAVDGPAHDGRESPDSREVSATPMDLTPPAKPKGLTVVSGVDRVFL
                     TWNENHETDLAGYRVYRSTRSGRGYERLTEKPLNRTTYSDESVKGGAVYYYVITAVDR
                     SGNESARSAEKKASVEKLR"
     gene            3123..>3883
                     /locus_tag="VLE22_13455"
     CDS             3123..>3883
                     /locus_tag="VLE22_13455"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSB15460.1"
                     /translation="MHDFQYKNGELYCEDVPLAAIARRVGTPFYVYSGNTLVNHFRAF
                     DRAFAGVPRLICFALKSNSNGAVLRLLGREGAGADIVSGGELFRALLAGIGPRKIVYA
                     GVGKRRDEIAYALNTGILMFNVESGDELQALNEVAGEMGVKARIALRVNPDIDPKTHA
                     YISTGLRENKFGIPIEHALEHYLTARSLPNVEIVGVHHHIGSQITEVGPFVEALDKVA
                     GLVTELRAAGMNIAYIDVGGGLGITYKDETPPHPQ"
BASE COUNT          849 a         1190 c         1194 g          650 t
ORIGIN      
        1 cgcgtcgacc tcgtcgaaaa ccgctacgtc ggcatgaagt cccgcggcgt ttacgagacg
       61 ccgggcggca cgctcctcca cgccgcgcac cgtgccgtcg aatccatcac catggaccgc
      121 gaggccatgc acctgcggga cagcctcatg ccgcgctacg cggaactggt ctattacggc
      181 tactggttcg cacccgagcg cgctatgctc caggcgatgg tggacgaatc gcagaagaac
      241 gtcacgggca cggcgcgcat caggctgtac aagggaaact gcgacatcgt gggccgcaaa
      301 tccgccgtgt cgctctacaa ccccgaactc gccaccttcg aggcggacgc ggtatacaac
      361 cagaaggacg cggaaggctt catccggctg aacgcgcttc ggctgagggt ggaagcaaga
      421 acacgaaagt gagagggtgg gaaggtaaaa tggtaagaaa gtgtcgtatt cacgccttct
      481 caacttcttg ccttcctacc ttccgtggtg acgaataaac atgacacgca gaacggcgaa
      541 aaagaaaacg aactccaaag agcagccggg acgcaagccc tggggcgggc gattcacgga
      601 ggccacgaac cggctcgtcg agaagtatac cgcttcgatc ccctacgatt ggcgtctgta
      661 tccctatgac atcgccggca gcacggcgca cgcggcaatg ctcgcgaaga ccggcatcat
      721 cacgagaaaa gaggcggccc ggatcatccg aggcctggac gagatccaga aggagatcac
      781 aggcggctcc ttcccgttca gcatcgagct cgaagacatc cacatgaaca tagagaaccg
      841 gttgatcagg atgatcggcc cggtcggcgg caagctccac accgcccgga gcaggaacga
      901 ccaggtggcc ctcgacgtcc gcatgtacct gcgcgacgag atcgccgaac tccacgaact
      961 tgcgggcgcg ctgcagaagg tgctcgtggg cctcgccgac gcgcaccgcg acgcggtgct
     1021 tccgggatac acgcacctgc agcgtgccca gcccgtgctc ctgggacacc acctgctcgc
     1081 ctactacgaa atgttcgagc gcgaccgcga ccgtctcaag gattgcttcc gtcgcgtaaa
     1141 catcctgccc ctcggagcgg gcgcgctggc cggcacggtg ctgccgatcg accgcaggtt
     1201 cgtggcccgg cagctgggtt tcagcggtct ttgcgagaac agcatggacg ctgtctcgga
     1261 ccgcgatttc gcaattgaat ttgccgctga ctgcgcccag atcatgatgc acctttcccg
     1321 cctgtgcgag gaactggtta tctggtcgtc ggcggaattc gggttcatca ccctgccgga
     1381 cgccttcacg actggctcgt cgatcatgcc gcagaaaaag aaccccgacg tggccgagct
     1441 tgcccgcgga aagaccggca gggtctacgg cggccttatg gcgctcctga ccatcatgaa
     1501 gggcctgccg ctcgcctaca accgcgacat gcaggaggac aaggaacccc tgttcgacgc
     1561 ggcggacacg gtcgcccatt ccctcgccgt ggtggccgaa atgctcaagg gtgtcgtgtt
     1621 tcacaaggaa gccatgcgga aggctgccgg agacggcttc atcaccgcaa ccgaccttgc
     1681 cgactacctt gtcaggaagg gaatgccttt ccgccgggcc catgagatcg tgggcaaggc
     1741 ggtgctgcgc gctatggaac tgggctgcgg cctccaggac atgccgctcg cggaataccg
     1801 gaagctgtct cggcagatca acagggacgt gttccaggcc atctcgctcg aatcgtcggt
     1861 gggcaggaga aggtcctacg gcggcaccgc tcccggaaat ctggacaagc ggctggaata
     1921 tctcaagaag aaaatgaaat gaagacccgt taccggtggt gcgtcgcggt ctgtctgctt
     1981 gcgctcgctg ccgcctcagc ctgcggcaga aaaacaaatc cgcttgttcc cgacagcccg
     2041 agacccgagg cgataaaagt cattaaggcc gtgtcgcggg gcggcgttgt gtacctgacc
     2101 tggcagatgc cgacgaggaa catcgaaggc aagagttacg acccttcagg catccggaag
     2161 attttcattg accgcgccga acccagtgag gaccataaga aagcgcggta caagcggtcc
     2221 gcggagatcg acgtggctga catgccgccg tcccttcttc gcgacaacgt ggcaacctgg
     2281 tccgacgggc aggtccggta cggccagaca tacgcatacc ggctgagggc cgtgagtgca
     2341 cacgggatca gcccgtggtc cgaaccggtc ctgatcgtcc cgcaggcgtc cctggccgca
     2401 ccgaaagggt tgacggcgac gaagggggac agcagcgtcg gactcacttg ggatccggtc
     2461 aggaccttgc tggacggcgg gcctgccgag ggcttcatag gatataatgt ctatcgaggc
     2521 acggataaag gaattcacga cgaagcaccg ctgaacaggg aaccgctcac cgctacgtcg
     2581 ttcaaggacg cgactgcggt gaacgaacac acgtattact acatcgtacg cgccgtagat
     2641 gggcccgcgc acgacgggcg ggaaagcccg gactcccggg aagtgtcggc cacaccgatg
     2701 gacctgaccc cgcccgcaaa gcccaaggga ctcaccgtcg tgagcggcgt ggacagggtc
     2761 ttcctgacct ggaacgaaaa ccacgagacc gaccttgccg ggtaccgcgt gtaccgctcg
     2821 accaggagcg gccgggggta cgagcggttg acggaaaaac cgctcaaccg gacgacatat
     2881 tcggacgaaa gcgtcaaggg cggcgcagtc tattattatg tgatcactgc ggtggaccgg
     2941 tccggaaacg agagcgcccg gtcggcagaa aagaaggcat ccgtcgagaa gctgaggtag
     3001 gatacaaatc ctgaatacga atttctacat cctaaacaag gacaaatctc aaagtgacgt
     3061 attttgtgtt ttgaaattgt ttagaatttc gggcttagaa tttagagctt tattaataaa
     3121 ccatgcacga tttccagtat aagaacgggg aactttactg cgaggacgtt ccgctcgccg
     3181 cgatcgcccg ccgcgtggga acgcctttct acgtctacag cggcaacacg ctcgtaaacc
     3241 acttccgggc cttcgatcgc gcattcgccg gagttccccg gctcatctgc ttcgcgctca
     3301 agtcaaactc gaacggcgcg gtgttgaggc tcctgggccg ggaaggcgcg ggcgccgaca
     3361 tcgtctccgg cggcgagctc tttcgcgccc tcctcgccgg aatcggtccg aggaagatcg
     3421 tctacgcggg cgtgggcaaa cgccgggacg agatcgcata cgccctcaat acgggcatcc
     3481 tcatgttcaa cgtcgagtcg ggggacgaac ttcaggcctt gaacgaggtc gccggagaga
     3541 tgggcgtgaa agcgcggatc gcgctcagag tcaacccgga catcgacccc aagacccacg
     3601 cctatatttc cacggggctc agggaaaaca agttcggcat cccgatcgag catgcgctcg
     3661 agcattacct gacagccagg tcgctgccga acgtcgagat cgtcggcgtg caccaccata
     3721 tcggatcgca gatcaccgaa gtggggccgt tcgtggaggc cctcgacaag gtagccgggc
     3781 tcgtgacgga gctccgcgcc gccggcatga acatcgcgta catcgacgtc gggggtggcc
     3841 tcggcatcac ctacaaggac gagacgccgc ctcatccgca gga
//
LOCUS       DATIFQ010000287        13775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1434335, whole genome shotgun sequence.
ACCESSION   DATIFQ010000287 DATIFQ010000000
VERSION     DATIFQ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13775
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434335"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VLE22_13460"
     CDS             <1..231
                     /locus_tag="VLE22_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15461.1"
                     /translation="CADIKELERGLSLFVSADINSVPSDAAAPMVAPTIRQNRWSSLT
                     IVPLKKPTLIFSSDDPTTKRQMQLELTATPIT"
     gene            complement(279..354)
                     /locus_tag="VLE22_13465"
     tRNA            complement(279..354)
                     /locus_tag="VLE22_13465"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Ala,seq:ggc)
     gene            387..950
                     /locus_tag="VLE22_13470"
     CDS             387..950
                     /locus_tag="VLE22_13470"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HSB15462.1"
                     /translation="MVDREQAWQILCEFTKNENLRKHALAVETCVVAYARKFGEDETT
                     WSVTAVLHDFDWEIHPQLPDHPLKGEPILAERGVPEEIRRAILSHASFSGVPRQSLLE
                     KTLFACDELAGFLTAVSLVKPRKSIFEVDVPSVRKKMKDKAFARAVNREDIVNGAAEL
                     GVPLDEHIDFCIRALQARAGDLGLQGI"
     gene            complement(955..1725)
                     /gene="mutM"
                     /locus_tag="VLE22_13475"
     CDS             complement(955..1725)
                     /gene="mutM"
                     /locus_tag="VLE22_13475"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HSB15463.1"
                     /translation="MPELPEVETVARTLAPRMAGRRIVGAEFPGRRVLRGEVPDLAGR
                     RIVGVRRHGKLVAVQCDGGVLAIHLGMTGRLLAGGERGPYTRAVLALEDGVVMFDDIR
                     QFGSVRWLEKPPEALGPDPLEIPAADLASRLGARRGRIKPLLLDQSFLRGIGNIYADE
                     ILFRARIHPRAIARRLSAGRARALHAAMRDVLGEAIERGGSSISNYVDSEGRKGSFQL
                     LHRVYRKTGLPCPECGTPIRRIVMTQRGTHYCPRCQRA"
     gene            1725..2705
                     /locus_tag="VLE22_13480"
     CDS             1725..2705
                     /locus_tag="VLE22_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSB15464.1"
                     /translation="MSYNNRTMRRLMALAMLLFLTLAACRREPERVIGVIPKGANHIF
                     WQTVHAGAIKAALESGYKIEWNAPALEIDSSRQIEIVESMVNRRLAGIVLAPVDRRAL
                     IAVVERAARERVPVAIFDSGIDTDRRITYVATDNTEGGRIAARRLGEVLGGKGKVGII
                     GFMPGSASTMEREDGFQDEMRKRFPEVRIVGLQFGMADRAKAMAATENILTAHPDLAG
                     LFADNESSSSGAVQALKSRGAREVKLVAFDASEQLIADLKAGWFDSLVVQNPFKMGYE
                     STRAVIDHLAGRKPPALVDSGVTLVRASDLDLPDVRALLFPDIDRYLKKP"
     gene            complement(2757..3923)
                     /gene="galK"
                     /locus_tag="VLE22_13485"
     CDS             complement(2757..3923)
                     /gene="galK"
                     /locus_tag="VLE22_13485"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HSB15465.1"
                     /translation="MDGAARIVAETFEQRYGAAVKPRVYRAPGRVNLIGEHTDYNLGF
                     VLPVALDLASFTACAPNGDGCFRVFSRNLDQERSWPVQSIAEAQPAKEWSDYPLGVAR
                     ELVRAGFPIQPYNLVIWSTVPTGSGLSSSAALEVSTALALLGGRAMEPLELAKLCRRA
                     ENDFVGMPCGIMDQYISVFGREHSAIRIDCRNLENEVVPLPEDVEFVAVNTMVKHELG
                     SSAYRQRVAECRQAVDVIRERLPEVASLRDVSSYVLSGFEAEMPIVPMRRALHVVTEN
                     ERVEAFVRASHEGDARTMGDLFVGSHRSLQKDYEVSCEELDFLVDTALAIPGVLGARM
                     TGGGFGGCTVNMIRPDVVELFKSAITHSYKARYGLTPEFYPCRPSAGAAEIAGV"
     gene            complement(3899..4084)
                     /locus_tag="VLE22_13490"
     CDS             complement(3899..4084)
                     /locus_tag="VLE22_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15466.1"
                     /translation="MWDLRTPSGWFFVILGVILCAVGIVSPGARAPLTDVNVNLYAGI
                     SMLLFGGILLWMARRAS"
     gene            complement(4090..5739)
                     /locus_tag="VLE22_13495"
     CDS             complement(4090..5739)
                     /locus_tag="VLE22_13495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSB15467.1"
                     /translation="MTLTGVDYIIILVYFAFVIGIGWLLHKRVSTSEDFLTSARSVPV
                     WITSLAFVATNLGAQELIGMCASGAKYGIMTSHFYWVGAIPAMIFVGLFMMPFYYGSR
                     ARSVPEYLKLRYDEKTRGFNALTFAVMTIFSSGISMYALGLLFQLVLGWSFTASVMLS
                     ALIVLIYTFLGGLTSAIYNEVLQFFLIVLGFAPLAILSVWKAGGWEGMAARLPAVMTS
                     SWKYMSHPSQNPMGVDLFGMIMGLGFVLSFGYWCTNFLVVQRAMAADSMAGARLTPLI
                     GTFPKMILPFIVIVPGIAAVALMKMDVGYAIPMKGDTYNYDLALTTLMAKFYPAGMLG
                     VGLTGLMASFMSGMAGNVTAFNTVWTYDIYQSYIRRGAPDSHYLWVGRLSTIFGVILS
                     IGTAYLAQLYNNIMDLLQLVFGFVNAPLFATFLLGMFWRRATGHGAFCGLVAGTSAAA
                     LTHGLTVAEGKGGWLGAIHEFPSTMAQNFWVAIFAWSSCFVVTILVSLFTRPRPEAEL
                     RNLVYGLTELPREGGVVWYRRPTLIAIAAGIVCLLLNFLFW"
     gene            complement(6504..7844)
                     /gene="nhaA"
                     /locus_tag="VLE22_13500"
     CDS             complement(6504..7844)
                     /gene="nhaA"
                     /locus_tag="VLE22_13500"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HSB15468.1"
                     /translation="MVPVVNPRRITPQKTPIVTLVRPFQEFAGRETSGGILLLACTVV
                     ALVWANSPWAHHYTALWHTTFTVGLGSFNLSNELHFWVNDALMAVFFFVVGLEIKREL
                     LAGELASPRQAALPILAALGGAVVPALLYTFLNANGPGARGWGIPMATDIAFAIGVMA
                     LLGDRIPLGLKVFLTALAIVDDIAAVLVIAVFYTSNLAWGALGVAAFCLLLALAANRL
                     GVRHPLPYALIGAVLWMTVLQSGVHATIAGVLLAFMIPSRTTINQREFLGHGRTVLDH
                     FERAAETEPFDILSDIEQQTAVEALEDACEKVQPPLHRLEYALHPWVTFLIMPLFALA
                     NAGVSLSGDLGKVVAQPITLGVVLGLVLGKPIGVTLASWLAVRLGLASLPENVSWKRI
                     LGAGWLAGIGFTMSLFMTGLAFTDDAHLTAAKLGILIASLCAGIVGSVILVRRG"
     gene            complement(8356..8844)
                     /locus_tag="VLE22_13505"
     CDS             complement(8356..8844)
                     /locus_tag="VLE22_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15469.1"
                     /translation="MKTITRTKIYLPMAALIVTAALALPASAQQQVPFKGTFQGSDAT
                     TGVNIHETATGMATLMGQFSLTMDVTINPADLTDTGSAQWIAANGDSIHTTVAGFAIP
                     GDVVFTVWETHTVTGGTGRFSGVQGSFVVHRTHVVALSPDGTHVTFGSFEGTMTPPGA
                     TH"
     gene            9192..12071
                     /locus_tag="VLE22_13510"
     CDS             9192..12071
                     /locus_tag="VLE22_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSB15470.1"
                     /translation="MGTVYLAEQRDPIRRLVALKVVKLGMDTGQVLARFANERQALAM
                     MDHPNIARIFDAGATTKGRPYFVMEYIDGAPITGYCDRKRMTTGERLALFLAVCRAVQ
                     HAHQKGVIHRDLKPSNVLVTEQDGAPVPKVIDFGIARATDKWAVENTLLTQFGQIVGT
                     PEYASPEQADTMTGEIDEASDVYSLGVLLYELLIGVVPFDTAALRNAGLAEMLRIIRE
                     DEAPSLPRKLTLMGAAATDIAARRQTNPISLRRLVDGDLNSIAMKALEKARERRYASV
                     SDLAGDIQRHLEHRPVLASPPGTLYRTRKFLRRHRLAALGTAAGIVFLVMSGVTVWSL
                     ARESARRPKLTDKDTIVLADFDNKTGDPVFDDTLRQGLSVELQQSPYLALMSDRTVQQ
                     TLTLMGQPKIAKLTPEIAQQVCERTGSAAVLEGSIARVGSQYVLGLRARNCNTGSVLD
                     HEQIQAARREDVLNSLSQIARKFRIRVGESLATVEKHSTPLAEATTPSLEALKAYSMA
                     NKVNLSSGSAAAIPFFRRAVEIDPKFAMAHAYLGLLYSTIGESVLSAESTTKARQLRD
                     RVSDREKFFIDFSYDRQVTGNLEKAYQTLELWLQTYPRGGQPRPQGLLGGLSTHGTGR
                     FERAIEASKKEIADEPYSYPGYGGLASSYYFLDRFPEAESTLQQASEHKLENSMFLVY
                     RYMIAVLEGDNDKVGRVMALARGKRGSEHPLAHAEALALVRSGRLRAARRSSSRAVDL
                     ALQEGDSESAASYRAARAVWEAVCGNAAEGRRNAVAALELSKGRDVEYAAGLALGLSG
                     DSSRSQLLADDLEKRFPEDTFAKFTYVPVLRALAALGRGKSAGAVERLEIARPYELAV
                     NGLNFSHYYLGGLHSAYVRGEAFLAAHRYTDATAEFQKIVDHRGIVGLDPIGALAHLQ
                     LGRAYASSGDKAKAKAAYLAFIRFWKEADLAIPILKSAKAEYARL"
     gene            12133..12876
                     /locus_tag="VLE22_13515"
     CDS             12133..12876
                     /locus_tag="VLE22_13515"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB15471.1"
                     /translation="MTNGPTMNTLLGSSRFPITRWTLVVAAGDPHRKEARSALVSLCE
                     NYWYPLYAYLRRRGYPADHAQDLTQDFFVRVLEGRYLDRADPEKGRFRSFLLTSLKFF
                     VADEEDRHRAHKRGGGAVLPLEFSSGEKRYQREPAHDETPERIFERRWALAVLDRVVE
                     RLRNEFVQHGRPEHFQRLKVFLLGQSDAPYAALAREMNTSEGALKVAIHRLRKRYREL
                     FRQEIADTVADPVEVESELRFLAAVLTRK"
     gene            complement(13086..13201)
                     /gene="rrf"
                     /locus_tag="VLE22_13520"
     rRNA            complement(13086..13201)
                     /gene="rrf"
                     /locus_tag="VLE22_13520"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(13281..>13775)
                     /locus_tag="VLE22_13525"
     rRNA            complement(13281..>13775)
                     /locus_tag="VLE22_13525"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2845 a         4295 c         4113 g         2522 t
ORIGIN      
        1 tgtgctgaca tcaaagagct agaacgcggg ctttccctgt ttgtgtcagc cgacatcaac
       61 agcgtgccgt ccgacgcggc ggcacccatg gtcgccccga cgattcgtca gaataggtgg
      121 agttcgttga cgatcgtgcc gctgaagaaa ccgaccttga tcttctcatc cgacgacccg
      181 acgacgaagc gccagatgca actggagctt acggcaacgc cgatcacata gctgcgccgc
      241 ttcgttctgg gggctcttga tcagtaacag tgagaatttg gtggacctgg tcggattcga
      301 accgacgacc tcttcaatgc cattgaagcg cgctcccaac tgcgccacag gcccacggaa
      361 gggatcttct tcattatatt acagagatgg ttgaccgaga acaggcctgg cagatcctct
      421 gcgagttcac caagaacgaa aacctccgca aacacgccct cgccgtcgaa acgtgcgtgg
      481 tggcttatgc ccgaaaattc ggcgaagacg aaaccacctg gtctgtcacc gccgtcctcc
      541 acgatttcga ctgggagatc catccccagc tccccgacca cccactcaaa ggcgagccca
      601 tcctcgccga gcgcggcgta cccgaggaga ttcgccgcgc catcctctcg cacgcctcgt
      661 tctccggtgt gccccgccag tccctgctgg agaaaaccct gttcgcctgc gatgagctgg
      721 ccggcttcct caccgccgtc tctctggtca agccgcgcaa gagtatcttc gaggtggacg
      781 tcccgtccgt ccgaaagaag atgaaggata aggccttcgc ccgcgccgtc aaccgtgagg
      841 acatcgtcaa cggcgccgcc gagctcggcg ttcccctcga cgagcacatc gacttctgca
      901 tccgcgccct tcaggcgcgc gccggggacc tcggcctcca gggcatctga gccgtcacgc
      961 cctctggcac cgcgggcagt agtgcgtccc acgctgcgtc atcacgattc gccggatggg
     1021 cgtcccgcac tccgggcaag gcagccccgt cttccggtac accctgtgca gaagctggaa
     1081 gctcccctta cgaccctccg aatccacgta attcgaaatc gatgaacccc cgcgctcaat
     1141 cgcctcgccc agcacatccc gcatcgccgc gtgcagcgct cgcgcccggc ctgcactcag
     1201 ccgcctcgcg atcgccctcg ggtgaatccg cgcacgaaac aggatctcgt ccgcatagat
     1261 gttcccgatc ccccgcagaa aggactggtc gagcagcagc ggcttgatcc ggccccggcg
     1321 cgcgcctagc cgcgaagcga gatccgcggc cgggatctcc agcgggtcgg gccccagcgc
     1381 ctccggcggc ttctccagcc accgcacgga tccgaattgc cggatgtcgt cgaacatcac
     1441 cacgccgtct tcgagcgcca acaccgcccg ggtatacggt cctcgctccc cgccggcgag
     1501 cagccttccc gtcatcccga ggtggatcgc cagcacacct ccgtcgcatt ggaccgccac
     1561 cagtttgccg tgccgccgga ctcccacgat ccgccgccct gccaggtccg gtacctctcc
     1621 gcggagcacc cgcctgcccg gaaactcggc gcccacaatg cggcgtcccg ccatcctcgg
     1681 cgcaagcgtc cgcgccaccg tttcaacttc gggaagttcc ggcaatgtct tacaataacc
     1741 gtaccatgcg ccgtctcatg gccctcgcga tgctgctgtt tctaacgctc gccgcatgcc
     1801 gccgcgagcc tgagcgcgtt attggagtga tcccgaaggg cgccaaccac atcttctggc
     1861 agaccgtgca cgccggcgcc atcaaggccg ccctggaatc cggctacaag atcgaatgga
     1921 acgcgccggc tctcgaaatc gactccagcc gtcagatcga gatcgtcgaa tcgatggtga
     1981 atcgccgcct cgcggggatc gtccttgcgc cggtcgaccg ccgcgccctg atcgccgtcg
     2041 tcgagcgcgc ggcccgagag cgagtcccgg tggccatctt cgactccggt atcgatacgg
     2101 atcggcgcat tacgtatgtc gctaccgaca acaccgaggg cggccgcatc gcggcgcgcc
     2161 gtctgggaga ggtgctgggt ggtaagggaa aagtcggcat catcggcttc atgcctggga
     2221 gcgcgtcaac gatggagcgc gaggacggct ttcaggatga aatgcgcaag cgctttccgg
     2281 aggtccgtat cgtcggcctg caatttggca tggccgatcg ggccaaggcc atggccgcga
     2341 ccgaaaacat tctgaccgca catcccgatc tcgcgggcct gttcgccgac aacgaatcga
     2401 gttccagcgg cgccgtgcag gcgctaaagt cgcgaggcgc ccgcgaggta aaactggtcg
     2461 ccttcgacgc ttcagagcag ctcatcgccg acctcaaggc cggttggttc gattcgctgg
     2521 tcgttcagaa ccctttcaag atgggttacg aatcgacccg ggcggttatc gatcacctcg
     2581 ccggccgcaa gccgccggct ctggtcgatt ccggcgtcac gctcgtccgc gcctccgacc
     2641 tggacctgcc cgacgtccgt gcgctcctct tccccgacat tgaccgctat ctcaagaagc
     2701 cctaaggtgg cacaggcttc cgccggtggt ggtctccagg ctttttcagc gccctgtcag
     2761 acgccggcga tttccgcggc ccctgccgac ggccggcagg gatagaactc cggcgtcagc
     2821 ccgtatcgcg ccttgtagga gtgggtaatc gccgatttga acagttcgac gacgtccggc
     2881 cggatcatgt tgacggtgca gcctccgaaa ccgccgcctg tcatgcgggc ccccagcacg
     2941 ccgggaatcg ccagcgcggt gtccaccagg aaatccaatt cctcgcagct cacctcgtag
     3001 tctttctgga gactgcggtg ggagccgacg aacaggtcgc ccatggtgcg ggcgtccccc
     3061 tcgtggctgg ctctgacgaa cgcctcgacg cgctcgttct ccgtgacgac atggagcgcc
     3121 cgccgcatcg gcacgatcgg catctccgcc tcgaagcccg agagcacgta gcttgaaacg
     3181 tcgcgcaggc tcgcgacttc cggtagtcgc tcgcggatga catcgaccgc ctgccggcac
     3241 tcggccacgc gctggcgata cgccgacgag ccaagctcgt gcttcaccat cgtattgacg
     3301 gcgacgaact cgacgtcttc cggcagaggg accacctcgt tttcaaggtt ccggcagtcg
     3361 atccggatcg cggagtgctc gcggccgaac accgagatgt actggtccat gatgccgcat
     3421 ggcatgccga cgaaatcgtt ctcggcgcgg cggcagagct tcgcaagttc gagcggctcc
     3481 atcgcccgcc caccgagaag cgccagcgcg gtcgacacct cgagcgcggc cgaggaactg
     3541 agcccggaac ccgtcggtac ggtgctccag atcacgagat tgtacggctg tatcggaaaa
     3601 cccgcgcgga ccagctcgcg cgccacgcca agcggatagt cgctccattc cttggcgggt
     3661 tgggcctccg cgatgctttg caccggccac gagcgctcct gatccaggtt cctcgaaaag
     3721 acgcggaaac agccgtcgcc gttcggagcg caggctgtaa aagacgccag gtcgagcgcc
     3781 accggcagga caaacccgag gttgtagtcg gtatgctcgc cgatcaggtt gacgcgcccc
     3841 ggcgcccggt aaactctagg ctttacggcc gcgccgtacc tttgttcaaa ggtctcggct
     3901 acgatgcgcg ccgcgccatc cataacagga ttcctccaaa aagaagcatc gagataccgg
     3961 cgtagaggtt gacgttcacg tcggtaagcg gcgcgcgggc gcccggagag acgattccga
     4021 cagcacagag gattacgccg agaattacga agaaccaacc ggacggtgtt cgaagatccc
     4081 acataccatt taccagaaca ggaagttcag cagcaggcag acaatccccg cggcgattgc
     4141 gatcaaggtc ggcctgcgat accagacgac gccgccctcg cgcggaagct cggtgagccc
     4201 gtacacaagg tttcgcaact cggcttcggg tctcgggcgg gtgaacaggc ttaccaggat
     4261 cgtaacgaca aagcaggacg accaggcgaa gatggccacc cagaaattct gcgccatcgt
     4321 tgacgggaac tcgtggatgg cgccgagcca gccgcctttt ccttcggcga ccgtgagtcc
     4381 gtgggtcagc gccgccgcgc tcgtgccggc gaccagcccg cagaaggcgc cgtggccggt
     4441 ggcccgccgc cagaacatcc cgagcaggaa tgtggcgaac agaggcgcgt tcacgaaccc
     4501 gaacaccaac tggagcaagt ccatgatgtt gttgtaaagc tgcgccaggt acgcggtgcc
     4561 gatcgagaga atcacgccga agatcgtgct caggcgtccg acccaaagat agtgcgaatc
     4621 gggcgcaccg cgccggatgt agctctgata gatgtcatac gtccagaccg tattgaacgc
     4681 ggtcacgttg ccggccatgc ccgacatgaa cgaggccatc agcccggtca ggccaacgcc
     4741 cagcatgccc gccggataga acttggccat cagtgtcgtg agggcaaggt cgtagttgta
     4801 cgtatcgccc ttcatcggga tcgcgtaacc gacgtccatt ttcatcaggg ccacggcggc
     4861 gatgccgggc acgatcacga tgaacggcag gatcatcttc gggaaggtgc cgatcagcgg
     4921 agtgagtcgc gcgcccgcca tcgagtccgc cgccatcgcc cgctggacca ccaggaagtt
     4981 cgtacaccag taaccgaacg agagcacgaa tcccaggccc atgatcatcc cgaacaaatc
     5041 gacgcccatc gggttctggg agggatggga catgtatttc cacgacgacg tcatcaccgc
     5101 gggcagacgc gcggccatgc cctcccaccc cccggccttc cagacggaca ggattgccag
     5161 cggggcaaag ccgagaacga tgagaaagaa ctgaagcact tcgttataaa tggcgctggt
     5221 gaggcccccg aggaacgtat agatcagcac gatcagcgca gacagcatca ccgacgccgt
     5281 gaagctccat cccagcacga gctggaacag cagacccagc gcgtacatcg agatgcccga
     5341 ggagaaaatc gtcatcaccg caaaggtcag cgcgttgaat ccgcgggttt tctcgtcgta
     5401 gcgcagcttc aggtactcgg gcaccgagcg cgcgcggctc ccgtaataga agggcatcat
     5461 gaacagcccc acgaagatca tcgcggggat tgcgcccacc cagtagaagt gggaagtcat
     5521 gatgccgtat tttgcgcccg aagcgcacat cccgatcagt tcctgcgcgc cgaggttagt
     5581 cgccacgaac gcaagactgg tgatccagac gggaacggaa cgggcggagg tgagaaagtc
     5641 ctcgctcgtc gaaacgcgct tgtgcagcag ccagccgatc ccgataacga aggcgaaata
     5701 gacaaggatg atgatgtagt caacaccggt gagggtcaat cggcacctcg aattacttct
     5761 ttcacgaaac gaatgagtct atcacgaaaa ccgtgcgggc cttgctgctc agcggggggt
     5821 gcgcgacaca gaagtggaga cgagcgaacg tctgataccg agccgcgacc gcaagggagc
     5881 ggttagctgc gtcacgtcaa aggccttccg cgtcgaacag tgccatgggc tcgccctgct
     5941 cgtcgattac gtactgaaga gcatgcctga catcctcgtc cttccacagc caacgggtgc
     6001 ttccatagca aagggtaggt tttgagacgc cggcggaagg ccctgaaatc aaggtcctca
     6061 gcgtctcaga agtcggtctc ggaatgttcg ccaagcagcg gttgtcacag cgttggcgtt
     6121 gcctgcggcc ctgtcgcgaa atcctatccc aaaaagagac gacgacttca cctggagcca
     6181 tcgggcattt ggtctccgca cggcccacgt tgatggtttg tcgcccacac gtagatgagg
     6241 cgtcttgccg tcgccaccga tcgtgaacgg ttgacggccc actcggtggc aatgccgctg
     6301 catccggcaa agcagcctcg gccacctggt cggcaaaccc gctcaccatg ctcgggcgcg
     6361 gcgggacgga gtcctaccca tggaatctta cacgggcgat cgcagtggag ggccgcggtg
     6421 gaaatggaaa tcggcgttct ggatcggtgg cgacacgcga gtaaaccttg gagacctccg
     6481 aaagcagcat ctgtgtgtcg aggtcaaccg cggcgcacga gaatcaccga accgacgatt
     6541 ccagcgcaaa gggaggcaat caggatgccg agttttgccg ccgtgaggtg cgcgtcatcg
     6601 gtgaaagcca aacccgtcat gaaaagcgac atcgtgaacc cgatcccggc tagccaaccc
     6661 gcgccgagaa tacgcttcca tgagacgttc tcgggaagag acgccagacc caatcgcacc
     6721 gccagccatg aggcaagtgt tacgccgatc ggtttcccaa gcaccagccc gagcacgaca
     6781 ccgagcgtga tgggttgggc cacaaccttt cccagatcgc cggagagtga cactcctgcg
     6841 ttagccaacg caaagagggg cataatcaga aacgtaaccc acggatgaag ggcatattcc
     6901 aacctgtgaa gcggcggttg cactttctcg caagcgtcct ccagtgcttc tacggcggtt
     6961 tgctgttcaa tgtcgctaag aatgtcgaaa ggttcggttt ccgcggctct ttcaaagtgg
     7021 tcaagtaccg tgcggccatg ccctagaaac tcacgctggt tgatagtggt gcggctcggg
     7081 atcatgaagg caaggagtac accggcaatg gtcgcgtgaa ctccggactg caacacggtc
     7141 atccacagca ccgcgccaat caatgcgtag ggaagcggat gccgcacccc gagacgattt
     7201 gccgccagcg ccaacaggag acagaaagcc gcgactccga gcgctcccca tgccagatta
     7261 gacgtgtaga acacggcgat gacaagcacg gcggcaatgt catcgacaat cgccagcgcc
     7321 gtcaaaaaca ccttgagtcc aagcggtatg cgatcaccga ggagggccat cactccaatg
     7381 gcaaatgcaa tatcggtagc catcgggatg ccccagccgc gagcgcccgg cccgtttgcg
     7441 ttgaggaaag tgtacagaag ggccggaacc actgcgccac ccaacgccgc caaaatggga
     7501 agcgccgcct gacgcggaga ggccagctcc cctgccagca gctcccgctt gatttccaaa
     7561 cccacaacga agaagaacac ggccatgagc gcgtcgttca cccagaagtg aagctcgttg
     7621 ctcaaattga aactgcccaa ccccaccgtg aacgtcgtat gccacaaagc cgtgtagtgg
     7681 tgcgcccacg gtgagttggc ccagacaaga gccactaccg tacaagccaa cagcagaata
     7741 ccgccggagg tttcccggcc ggcaaactcc tgaaagggcc gcacaagcgt cacgatcggc
     7801 gttttctgtg gcgtgattcg tcgcgggttg accacaggaa ccattgtccc aggaaaatta
     7861 tcgtttcaag taagtgaagc gttgagccgt cggtctgctc ggtttccccc gagcaccggg
     7921 ccgatagctt cggacaatcc ggccttcagt cacttaaacg gagagaggtt gccccgccga
     7981 tgggtgtgat gatgacgctg gcggatctgt tcttcttgcc cctcaacaag agggactccc
     8041 cgcgatggcg ctgatgatgg gtctgatggc ggctattgtc gcggccgtta tcatcttcct
     8101 ttcctgatca ttagagaagc gacagagatt gcgaaatctc gaacgcgcgc atgcatcgga
     8161 tggatgccac cgtcacccca ttttgttgac gtcataggcg aggttcccga acccgggcgc
     8221 gatcccgcct agatctgtga ccgcctgaaa aatttctaaa gaaatctttc aagcggtcga
     8281 ggacctaggt gcttggagaa atggcgtcag acgcctctac tcggcatcgc ttctccgcgc
     8341 aatcattgac tgccctcagt gagttgcgcc cgggggagtc atggtcccct cgaacgagcc
     8401 gaaggtgacg tgcgtgccgt cgggactgag tgccacgacg tgcgtgcggt gcacgacaaa
     8461 gctcccctgg acgcccgaga atcgacccgt tccgccagta acggtgtgag tttcccagac
     8521 cgtaaagaca acatcaccgg gtatagcgaa cccggcaacc gtcgtatgga tgctgtctcc
     8581 attggccgcg atccactgag ccgaccctgt gtcggtgagg tcagcggggt ttattgttac
     8641 gtccatggtc aatgagaatt gccccattag ggtggccatt cccgtcgcgg tttcatggat
     8701 gttgacccct gtggttgcgt cactcccttg gaacgtaccc ttgaagggta cttgttgctg
     8761 tgccgacgcc gggagggcaa gcgccgccgt cacaatgagc gccgccatcg gcaggtagat
     8821 cttggttctc gtgatggttt tcatatgtcc gttcatctcc gttcaggttt ttcgaccagg
     8881 cgccggttcg aatccgtctc gcctgccacg gataaggaat cgcggcgctg aaggttacag
     8941 cgctcggcgc gaagggcgtt agaataggac ctgggactct ggtcccgatg ccaaactgcc
     9001 ctgggtgcgg agagcaattg ggttccggtg atcccgccgg actgtgcccg aagtgtctga
     9061 tcctgggcgc tttcgagagt tggactggcg cggaagaatc cagcactcag accaccggta
     9121 ccgcgccagc ggaagcctac gatgacgact tcggccgtta tcggatcgtt cggccgatag
     9181 gcgaaggcgg catgggcacc gtgtacctgg ctgagcagcg cgaccccatt cgtcgtcttg
     9241 tcgccctcaa agtagtgaag ctcgggatgg acaccggcca ggtgcttgcc cgcttcgcca
     9301 acgagcggca ggcgctcgcg atgatggacc acccgaatat cgctcggatc ttcgacgcag
     9361 gtgccaccac caaagggcgc ccgtacttcg tgatggagta catcgatggt gcgccgatca
     9421 cggggtattg tgatcgcaag cggatgacca ccggggagcg gctcgcgtta ttcctcgcgg
     9481 tctgccgtgc ggtccaacat gcgcaccaga agggtgtgat acaccgcgac ctgaagccgt
     9541 cgaacgtgct ggtgaccgag caggacggcg cgccggtccc caaggtaatc gacttcggca
     9601 tcgcgagggc caccgacaag tgggccgtcg agaacacgct gctcacgcag ttcggccaga
     9661 tcgtcgggac cccggagtat gccagcccgg agcaggcgga cacgatgacg ggagagatcg
     9721 acgaggcctc ggatgtgtat tcgctcggcg tgctgttata cgaactgctg atcggagtgg
     9781 tgccgttcga cacggccgcg ctgcgcaatg ccggtttagc ggaaatgctg cgaataatcc
     9841 gcgaagacga agcgccatca ttgccgcgaa agctgacctt gatgggcgcg gcggcgaccg
     9901 acattgccgc gcgtcgccaa accaacccaa tttctctgcg ccgcctggtc gacggcgacc
     9961 tgaactcgat cgccatgaag gcgctggaga aggcgcggga gcggcgctat gcttcggtgt
    10021 cagatctcgc gggcgatatc cagaggcacc tggagcatcg gccggtgctg gcctcaccac
    10081 cgggaaccct gtaccgaacg cggaaatttc tccgcaggca caggctggcc gccctcggca
    10141 cagccgcggg tatcgtattc ctcgtgatga gcggagtgac ggtctggtcg cttgcgcgcg
    10201 aatctgcgcg gagacccaag ctgaccgaca aggacaccat tgtcctcgca gatttcgaca
    10261 acaagaccgg cgatccggtg tttgatgaca cgctccgcca gggactgtct gtggagcttc
    10321 aacagtcgcc gtacctcgcc ctgatgtcgg acagaacggt ccagcagacg ctgactctga
    10381 tgggacagcc caaaatagcc aagctgacgc cggaaatcgc tcagcaggtc tgcgagcgaa
    10441 ccggcagcgc ggctgtcttg gagggctcga tcgcacgtgt tggaagccaa tatgtgctcg
    10501 gcttgcgcgc caggaactgc aatacaggca gtgttctgga tcatgagcag atccaggcgg
    10561 caaggcgaga agacgtcctc aactctctca gtcagatcgc ccgtaaattc cgaatcaggg
    10621 tgggcgaatc gctggcaacg gtggaaaagc actcgacacc gcttgctgag gccacgacac
    10681 cgtcactcga agcactgaaa gcttacagca tggccaacaa ggtaaactta tcctccggga
    10741 gtgccgcggc gatacccttc ttccggcgag cggtcgaaat tgatcctaag tttgccatgg
    10801 cgcacgccta tttggggctc ttgtacagca ctattggaga gtcggtgttg tcggcggaga
    10861 gcaccacaaa agcccggcaa ctgcgggatc gtgtcagcga tcgggagaag ttttttatcg
    10921 actttagcta tgaccggcag gtgacgggaa atctggaaaa ggcctatcaa acgctcgagt
    10981 tgtggcttca gacttatcct cgcgggggac agccaaggcc gcagggtttg ttagggggtc
    11041 tttcgaccca tgggacaggc cgatttgaaa gggcgatcga ggcatccaaa aaagaaatcg
    11101 cggacgaacc ctactcttac cctggatacg gcggtcttgc gtcgagctac tatttcctcg
    11161 accgcttccc cgaggccgag agcactcttc agcaagcctc cgaacacaag ctggagaact
    11221 ctatgtttct ggtgtatcga tacatgatcg cggtattaga gggtgacaat gacaaggtgg
    11281 gccgggtaat ggctctggcc agaggcaagc gtggatcgga acacccgctg gctcacgcag
    11341 aggctctggc cctggttcgt tccggccgct tacgggccgc ccgtcggtca tccagccgcg
    11401 ccgtggatct ggccctgcaa gagggggata gcgagtcggc agcgagctac cgggccgcac
    11461 gagcggtgtg ggaagccgtt tgcgggaatg ctgccgaagg caggaggaac gccgtggcgg
    11521 cgcttgaact ttccaagggc cgggatgtcg aatacgccgc cggccttgcc ctgggtcttt
    11581 cgggggactc ctctcgatcg caactgcttg ccgatgatct ggaaaagcgc ttcccggaag
    11641 ataccttcgc caaatttacc tacgtaccgg ttctccgcgc gttagccgca ctggggcggg
    11701 gcaagtccgc aggcgccgtg gagcggctgg aaatcgctcg tccgtacgag ctggcggtga
    11761 atggcctgaa cttcagtcat tattatcttg gcggtttgca ctcggcctat gtccgcggag
    11821 aggccttcct tgccgcgcac cggtatacgg acgcgacggc cgagtttcag aagattgtcg
    11881 atcatcgcgg tattgtgggc ctggatccca tcggcgcgct ggcgcacttg caactcggga
    11941 gagcgtacgc gtcatcagga gacaaggcca aggcgaaagc tgcttacctg gctttcatcc
    12001 gattttggaa agaggcagat ctcgcgatcc cgatcctgaa aagtgccaaa gcagagtatg
    12061 ccaggctgta atgattgcgc tgtaaccttt ggcttccgat tccttattca tgttgcccgt
    12121 gaggataaga ccatgacgaa cggcccaacc atgaacacgt tgcttggatc atcccggttt
    12181 ccgataacgc gatggaccct cgtggtcgcc gccggcgatc cgcatcggaa ggaggctcgg
    12241 tccgctctgg tatctctgtg tgagaactac tggtatccgt tgtacgccta cctgcgccgg
    12301 cgaggttatc cggcggatca cgcgcaggat ctcacgcagg acttcttcgt ccgggtgttg
    12361 gaagggcgat atctcgatcg cgctgacccg gaaaagggtc ggttccggtc gtttctgttg
    12421 acttcgttga agttctttgt cgccgacgaa gaagaccgcc atcgcgcgca caaacgtggc
    12481 ggcggggcag ttctgccgct tgaattctca tccggcgaaa agcgctacca acgcgagcct
    12541 gcgcacgatg agacccccga gcggatcttc gaacgacgct gggcgcttgc ggtgcttgac
    12601 cgggtcgtgg agaggttgcg gaacgagttc gtgcaacacg gccgcccgga gcatttccag
    12661 cgattgaagg tgttccttct cggccagtcc gacgcgccgt atgccgcgct ggcgcgcgag
    12721 atgaacacct cagagggagc gctcaaagtt gcgattcaca ggcttcgcaa gaggtatcgc
    12781 gaactctttc gacaggaaat cgcggatact gtggccgatc cagtcgaagt ggagtccgaa
    12841 ctccggtttc tggcggccgt gctgacgagg aagtagcggc cggccgcccc gagtcggttc
    12901 agtgtggtcg aaaccccatg cagcaaccag tttcaaaagg aaaacagggg ctgtcgatca
    12961 tattccgcat aatcggaggc tctaccgttt cttcagccaa ccgcctcgtg ccaaagcacc
    13021 cacagatttc taaccggcga ggcagaaaaa aagcccccgg aaaacccggg ggcttcttag
    13081 tttaatttgg gcgacgtcct actctcccac acactcgcgc gcgcagtacc atcggggctg
    13141 aggggcttaa ctgccgtgtt cgggatggga acgggtgaac cccctcgcta cagccaccca
    13201 aaagaccgat tgacggctcc caagagccat ccgaatatcg acgggcgcca aggtctcacc
    13261 ctgtgccaag ggcaaatttt atggtcaagc cgaacgggct attagtaacg gtaagcttca
    13321 cccgttgccg ggcttccaca tccgtcctat caacgtggta gtcttccacg gcccttctta
    13381 gccttgcggc ttgggagatc tcatctcggg gaaggtttca cgcttatatg cattcagcgt
    13441 ttatcctgac cgaacttcgc tacccagcga tgccactgga gtgacaactg gatcacaaga
    13501 ggttcgttca tcccggtcct ctcgtactaa agacaagccc cctcaaatct cctgcgccca
    13561 ccacagatag ggaccgaact gtctcgcgac gttctgaacc cagctcacgt accgctttaa
    13621 tgggcgaaca gcccaaccct tgggagcttc tacaccccca ggatgcgatg agccgacatc
    13681 gaggtgccaa accggagcgt cgatgtgaac tcttgactcc gatcagcctg ttatccccgg
    13741 cgtacctttt atccgttgag cgatggccct tccat
//
LOCUS       DATIFQ010000288         2260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_93116, whole genome shotgun sequence.
ACCESSION   DATIFQ010000288 DATIFQ010000000
VERSION     DATIFQ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93116"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VLE22_13530"
     CDS             <1..321
                     /locus_tag="VLE22_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15472.1"
                     /translation="EEQSYSAIPASSSRDDETLNISRFFPACPIIDTKARSLLIPLLI
                     RARRGSVLMTTSIAFTAYQVLNMEQISVFCLDGLEDPFSPFLVIDEWTSLHANPSQRR
                     ITPP"
     gene            318..566
                     /locus_tag="VLE22_13535"
     CDS             318..566
                     /locus_tag="VLE22_13535"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSB15473.1"
                     /translation="MNVEPSRQTKKTKPRTPASTPTPAAAPSAARTRQPLPPCDDPHI
                     LIAKRAYELYSERGYRHGYALDDWLEAEREILSQIPPV"
     gene            699..>2260
                     /locus_tag="VLE22_13540"
     CDS             699..>2260
                     /locus_tag="VLE22_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HSB15474.1"
                     /translation="MSYVGVNIGALTVKVAALRGDARNSTVLAHQGRPLEILDEILAR
                     PDFADAEYFGVSGQLGHISEVAAVQRALREVDDTFDAVASLGGESFLVYLLMDGRIGN
                     VVSHNKCAAGSGEFFVQQIGRMGLGMEEAIQRSFSGKVVPLASRCSVHCKSDITHKLN
                     RNEATPEDILHTLHDSMSNKVIALLEKGARDVKRVLLIGGVTRNDAMLAALRAKLPAT
                     EFVVLPESPWFEAWGTALLVRDSPVETTPKIAEQPGLGRLPPLHLYGDRVQVIAAPPR
                     QAPPDGPLILGVDAGSTTTKAILLDPSTQAVVASYYGRTKGNPVAATRECLQALIEQV
                     GNRPVGLIGTTGSARELAGAYLGTEHVYNEISAHAAGATHFDPEVDTIFEIGGQDSKY
                     IYLRNGVPIDYAMNNACSAGTGSFLEESARSDLGITVSDIADQALAAASPVHFKATCA
                     AFINSDIRLAQQQGHPRDNIIAGLVYAIASNYLNRVKGPRYVGKKVFLQGGVALNRAV
                     GYAFAHSVGRQVV"
BASE COUNT          448 a          738 c          670 g          404 t
ORIGIN      
        1 gaggaacaaa gctacagtgc catcccggct tcttcatcac gcgatgatga aaccctcaac
       61 atttcgcgat tcttccccgc ctgcccgatc atcgacacga aggcacggtc gttgctcatc
      121 cccttgctga tcagggcacg acgagggtcc gtcctaatga ccacttcgat agcctttacc
      181 gcctatcagg tcttgaacat ggaacaaata tctgtctttt gcttggacgg tctcgaagat
      241 cccttctcgc cttttcttgt tatcgatgaa tggaccagcc tacacgcgaa cccttcgcaa
      301 aggaggatca caccgccatg aacgtagaac cttcacgcca gacaaagaag accaagcccc
      361 ggacgccggc gagtacgccg actccagcag cagctcccag cgccgcgcgc acgcgtcagc
      421 cgctgccgcc ctgcgatgac ccgcatatac tgatcgcgaa gcgggcctat gagctctaca
      481 gtgagcgggg ctatcggcat gggtatgcgc tcgatgattg gctggaagcg gagcgcgaga
      541 ttctcagcca gatcccgccg gtctgacagg gccgcccaac gtactacgag tgtccgacag
      601 actcaccggg cggtgagtgg ttttgttcag ctcctgcttg aacaggcggt tgggtaagag
      661 cagcgatcgg cacgacgccg gcttatggag gagttggcat gtcctacgtc ggcgtaaaca
      721 tcggcgccct cacggtgaag gtggcagccc tccgaggcga tgcccgaaac tccaccgtgc
      781 tggcccatca aggccgcccg ctggagatac tcgacgagat tctggccagg ccggacttcg
      841 cggacgcaga gtacttcggt gtatccggcc agctcggtca tatttcagaa gtggctgcgg
      901 tccagcgggc gctccgcgag gtggacgaca cgttcgacgc agtcgcctcc ctcggggggg
      961 aatcctttct cgtctacctc ctcatggacg ggaggatcgg caatgtggtg tcccacaata
     1021 aatgcgcggc cggcagcggc gagtttttcg tgcagcagat cggccggatg gggctgggca
     1081 tggaggaagc gatccagcgc tccttcagcg ggaaggtcgt gccgctggcg tcacgctgct
     1141 cggtccattg caaatcagac atcacccaca agctgaaccg caacgaagcc acgcccgagg
     1201 atattctcca tacgctgcac gacagcatgt ccaacaaagt gatcgcgttg ctggaaaaag
     1261 gcgcacgcga cgtgaagcgg gtcctcctga tcggcggcgt gacgcgcaac gacgcgatgc
     1321 tggcggcctt gcgcgccaag ctgccggcca cggagtttgt ggtgctgccg gaaagtccct
     1381 ggttcgaagc ctggggcacc gccctgcttg tacgggactc acccgtcgag accaccccga
     1441 agattgccga gcaaccgggc ctcggccgcc tcccgccact ccatttatat ggcgaccggg
     1501 tacaagtgat tgccgccccg ccccggcagg ctcctcccga cggaccgctg atcctgggag
     1561 tcgatgcggg atcgaccacg accaaagcca ttttactcga cccctcgacg caagccgtcg
     1621 tggcctctta ctacggacgc accaagggga atcccgtggc ggccacccgc gagtgccttc
     1681 aagcgctcat cgagcaggtg ggcaatcgcc cggtcggact gatcggcacg accggatcgg
     1741 ccagagaact cgccggcgcc tacctcggca ccgagcacgt ctataacgag atctcggccc
     1801 atgcggcggg cgcgacccat ttcgatccgg aggtggacac gatcttcgaa atcggcgggc
     1861 aggactcgaa gtacatctat ttgcgcaacg gcgtgcccat cgactacgcg atgaataacg
     1921 cctgttcggc cggcaccggc tccttcctgg aggagagcgc ccgcagcgac ctcgggatta
     1981 ccgtatcgga catcgccgac caggcgctgg ccgctgcgtc cccggtgcac ttcaaggcga
     2041 cctgtgccgc cttcatcaat tccgacatcc ggcttgccca gcaacagggc catccccgcg
     2101 acaacatcat cgccggactc gtctatgcca tcgccagcaa ctatctgaat cgagtgaaag
     2161 ggccgcgcta cgtggggaag aaagtattcc tgcaaggcgg cgtcgcgctg aaccgcgctg
     2221 tcggctatgc cttcgcccac agcgtcggcc gccaggtcgt
//
LOCUS       DATIFQ010000289         8340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_285569, whole genome shotgun sequence.
ACCESSION   DATIFQ010000289 DATIFQ010000000
VERSION     DATIFQ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285569"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..1250)
                     /locus_tag="VLE22_13545"
     CDS             complement(306..1250)
                     /locus_tag="VLE22_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15475.1"
                     /translation="MTSTRRQFLRSGSALAGGLACGAAHGFGQSQRGQNQIASIPDSE
                     IRIPKVKFGKAEISRLILGVNPFYGFSHYNSNFSLAMRDWYTQAKVLEVLQRSEALGI
                     NAFNYVHMSRGEEDWKRYLDAGGKMQLVAQATTKEPAEVVNAVKPMAAWVQGEITDRA
                     YRSGSLETVRDYAKKMRDLGVAMVGIGSHIPEVLMKIEDLGWDVDFYAGCAYNIRRTP
                     QEFRQLLGGEIPEMQGDIYLQDDPPRMYNFMRQTKKPCVAYKILAAGRVSNPEQAFRR
                     ALESIKPADLVCVGVFPRTKDEIKEDAYYTSEYGATAS"
     gene            1449..2666
                     /locus_tag="VLE22_13550"
     CDS             1449..2666
                     /locus_tag="VLE22_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15476.1"
                     /translation="MEPSSRREFLKATAAVTTAASYGRILGANDAIRLGCIGNGGRGK
                     YLMRHLNSIGGVSWAAVCDVYSVRRDEAATIAGGSVKTFSDHRELLDLKDIDAVIVAT
                     PDHWHSAVTVDACKAGKDVYVEKPMVHTPKDGQALVKAVRQHKRICQVGMQARAMPHY
                     QEARQKYVESGAMGKVGLVRTWYDANGGYTLVAPPGMEKQPEGLDWKRWCGPGPQVPW
                     NPGIFFSPYKWLHYDGGMIMGIGIHVIDSAHQYMKLTRPAAAVAGGGVYHYNDGRDTP
                     DVINLILEYPEKLNVTFEAEIMTSGLKSSQAGIEVRGTGGILAVHRYENLGWQYTPNP
                     KNSTLPADKAPGGPPDADELLKNWLDCIRTRQKPVADEEAGYYSSIACFMGAMAYRTK
                     SRVEWDRKWELGA"
     gene            2677..4788
                     /locus_tag="VLE22_13555"
     CDS             2677..4788
                     /locus_tag="VLE22_13555"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF037021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSB15477.1"
                     /translation="MRATRLSLFLCAVLLAGCGRKSAPLYSFEAESEAQAAKASGATV
                     SRVDRGATDGKFALLVEFQPGDEPQIEFAAGEKLWDWKAKGSLILDVTNAAEEPAAFS
                     VEVTDAAGAKTVAKTFLPLRAGESASFALPLNSPSPLDMGMRGEPAIPGFRLLNGDHQ
                     TVDVSRIAKFRIFAAKLEKSRTLVIDNIRLGRGVKYEKIVDRFGQFGLEDWPGKLKSE
                     QELSKRRSEEEAELAARPVLPDRDEYGGWSAGPQLAAAGYFRTAKRDGKWWLVTPSGH
                     LFFSFGFNSVNSEEGDTVVEGREHMFEWLPKPGEPLSEHYGKNSMWMPVGLKIKLATG
                     RTYHYYRANLERKYGKDWYERWREITLKRLPSWGFNTIANWSDPRVYEARRIPYVVTI
                     DIDAKIAEVSSGNDYWRRMMDPFDPAFAAAVEASLRAGTEKRRDDPWCVGYFVDNELS
                     WGSMRDDRGRYGLALGALALDASSPAKQAFVEQLRKRRSAIGTLNRAWGTTFASWDAV
                     LGEPYQPQGELSAGMKDDLRAFVKEFARKYFRTIRDSLKKHDPNHLYLGTRFAWSSQE
                     SIEACAEICDVMSFNIYRPRVERSQWVSLEKLGKPVIIGEFHMGAVDRGMFHPGLVRT
                     EDQAARAALFSAYVRDVIDNPMFVGCHYFKYVDEPLTGRPGDGENYAIGFVNVVDGLH
                     TEMIEASRTLSGEMYKRRAGQ"
     gene            complement(4789..5946)
                     /locus_tag="VLE22_13560"
     CDS             complement(4789..5946)
                     /locus_tag="VLE22_13560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB15478.1"
                     /translation="MPAGSGDLVFLVAAFTLSIWFYLLLARGGFWRMREERLGACPSN
                     SPPRVAVIIPARNEAVSVAAAVTSVLLQDYPGLFQVFLVDDHSTDDTAHVARGAARDA
                     GKSGLLEVVPAPPLPPGWTGKLWALSEGIRRAESFQPDSYWFTDADVVHSPCTLAKLV
                     AKAESGWDLVSLMVKLRCETFAEKAAVPAFLFFFFKLYPPVWISDRGRTAAGAAGGCL
                     LARREALERAGGIEAIRSEVIDDCALAREVKRSGGKIWLGLTSASHSIRGYGTFTAVA
                     GMISRTAYTQLSYSSLLLAGTVAGMFVTYLLPPLLALTAKPPAAILGGLAWLLMSVTY
                     APSLRFYGRFVLWAPVLPLVALFYTGATVASAVRYWSGRGAAWKERFQATP"
     gene            complement(5927..7969)
                     /locus_tag="VLE22_13565"
     CDS             complement(5927..7969)
                     /locus_tag="VLE22_13565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HSB15479.1"
                     /translation="MRLTIWSLLLFLAASPVCPQSSSSVQPKEIVSFDLDAIDRGVNA
                     CTDFYEFACGNWRKNNPIPPDKARWGRFDELADRNLSLLREILEKAADAGAPGPERQI
                     GDFYAACMDEPRIESLGARPIQADLDAVAKIRNRRDLMRAVARLHTVGVPALLGWGST
                     ADLHDATRMVAVVDQGGISLPDRDYYIKDDVRSKETREKFLEHVSRMFGLLGRSQEDS
                     AAAAKTVLAVETALARASMDRTLRRDPKNRDHFMKLSELAKLAPNFDFPRFFEASRAP
                     KFDSLNVANPEFFKQVSAQLESVPVADWKTYLSWQVLSSRADYLSSLFVNESFRFNNQ
                     YLVGQKEIAPRWKRCVRLADVSLGEALGRIYVEKHFGADGKERTLRMVKAIESAMEKD
                     IAQIDWMSAATKEKARQKLAAIVNNIGYPDRWRDYSSIRIARDDLAGNVVRAAAFEER
                     RQVAKIGGPVDRTEWSMTPQTVNAYYRGSRNDINFPAGILQPPFFSRTIDDPVNFGAI
                     GLVIGHELTHGFDDQGRKFDASGNLTDWWTEQDATEFDKRAACVDEQYSQYVAVKDDK
                     GEVRLNGKLTLGENVADNGGLRLAHSALMDTLTDEGGKKTIDGFTSEQRFFIGYAQVW
                     CQNATDQISRLRALTDPHSPGRYRVIGVVANSPEFRDAFGCKPGDPMVRENACRVW"
     gene            complement(8031..>8340)
                     /locus_tag="VLE22_13570"
     CDS             complement(8031..>8340)
                     /locus_tag="VLE22_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15480.1"
                     /translation="SYVGYTSAGYVDESCGGLDSPCAGGATPPSDSPQLQIWATPFAK
                     FKGLIYQPRGAWTVVQAAGDYDGPLRIVSGALELQGTGRLTLNGESPPIINYVTALVE
                     "
BASE COUNT         1692 a         2430 c         2707 g         1511 t
ORIGIN      
        1 caaggacggc aagttgtctt ccctgcgggt gatgaacagc caggtcgatc cggatctggc
       61 gaagtcggct gtagaggcgg tgagccagtg ggagtaccag ccgacactgc tgaacggcaa
      121 tccagtggag attgtgaccg acatcgaggt gaactacaaa ctgagcaagt agggcttatt
      181 gagttcggcc ggcacctcgg ggaccggcag ccatttcgcg aaggcggcgg catggcggcc
      241 agtccccgtt tgcatttgcg gttatcgagt gattgcgtgt ccccgaattc gaattaagag
      301 cctcgtcagg aagccgtcgc accgtactcg ctggtgtagt aggcgtcctc tttgatctcg
      361 tccttagtgc gcgggaacac gcccacacac acgagatccg ccggcttaat gctctccaat
      421 gcccgccgaa aagcctgctc tgggttcgaa accctgcccg cggccagaat cttgtatgcg
      481 acgcaaggct tcttggtttg gcgcatgaag ttgtacatcc tcggcggatc gtcctggaga
      541 taaatgtcac cctgcatttc ggggatctcg ccccctaaca actggcgaaa ctcctgcgga
      601 gtcctgcgga tgttgtaggc acatcccgca tagaagtcca catcccagcc cagatcctca
      661 atcttcatga gaacctccgg aatgtgactg ccgatgccca ccatggccac gcccaagtcg
      721 cgcatcttct ttgcgtagtc ccggacagtt tccaggctcc cgctgcgata tgcccggtcc
      781 gtgatctccc cctgaaccca agcagccatg ggcttcaccg cattcaccac ctccgccggt
      841 tctttcgtcg tcgcctgagc aaccagttgc atcttgccgc ccgcatccag gtagcgcttc
      901 caatcctcct ctccccgact catgtggaca tagttgaagg cgttgattcc caaagcctca
      961 gacctctgga gcacctccaa aaccttggcc tgcgtgtacc aatcccgcat ggcgagactg
     1021 aaattgctgt tgtagtgcga gaacccgtag aacgggttga cccccaagat gagccggctg
     1081 atctcagcct tgccaaactt gaccttcggg atcctgatct ccgagtcagg aatagaagca
     1141 atttgattct gaccccgctg cgattgccca aagccatgag cagccccgca ggccagacca
     1201 ccagccaaag ccgaacctga cctcagaaac tgccttcgcg tactcgtcat gtggatgtac
     1261 ctcgctacca aatctacccg taccctgtgc atgctggcaa gttctcttgc acagcctgaa
     1321 ggctgtgcca ccaaagtgaa tcagtgacag taggcttcag cctctggctc aggtctttca
     1381 acatggcccg cacaaccatc gttggagccg gtataggata tagggctgcg gagaaacaac
     1441 caatggctat ggaaccatcc tccagaagag agtttctgaa agcgacggcg gcggtcacaa
     1501 cggcagcgtc gtacggccgc atcctcgggg cgaatgacgc gatccggctc ggctgcatcg
     1561 ggaacggtgg acgtgggaag tacctgatgc gtcacctcaa cagcattggt ggagtgtcgt
     1621 gggcagcggt ctgcgacgtc tacagcgtcc ggcgggacga agcggccaca atcgccggtg
     1681 gttcggtgaa gacattcagc gatcaccgag aactgctcga cctcaaggac atcgatgccg
     1741 tgatcgtggc gactcccgac cactggcact cggcggtcac agtagatgcg tgcaaggccg
     1801 gcaaggacgt gtacgtcgag aagccgatgg tccacacgcc gaaggatggg caggcgctgg
     1861 tcaaagccgt acggcagcac aagcgcatct gccaggtcgg catgcaggcc cgtgcgatgc
     1921 ctcactacca agaggcgcgg cagaaatatg tcgagagtgg cgcgatgggt aaggtgggcc
     1981 tcgttcggac atggtacgac gccaacggcg gctataccct ggtggctccc cctggaatgg
     2041 agaagcagcc tgaggggctt gactggaagc gatggtgcgg cccagggccg caggtgccgt
     2101 ggaacccggg gattttcttc agcccgtaca agtggctgca ctacgacggc gggatgatca
     2161 tgggcatcgg catccatgtg atcgactccg cgcaccagta catgaagctg accaggccag
     2221 cggcggccgt cgccggcggc ggagtctatc actacaacga cggacgcgat acgccggacg
     2281 tgatcaacct gatcctcgaa tacccggaaa aactgaacgt cacgttcgag gccgagatca
     2341 tgacgtccgg cctgaagtcc tctcaggcgg gaatcgaagt acggggaact ggcggcattc
     2401 ttgccgttca ccgctacgag aacctcggct ggcaatacac tcccaatccg aagaacagca
     2461 cactgccggc ggacaaagcc cctggcggac cgccggacgc agatgaacta ctgaagaact
     2521 ggcttgactg catccgcacc cgccagaagc cggtcgcgga cgaggaggcg ggttactact
     2581 cgtcgatagc ctgcttcatg ggcgccatgg cttaccgcac gaagtcgcgg gtggagtggg
     2641 accggaagtg ggagctcggt gcgtagggag aaggagatga gagcgacacg gttgtcgtta
     2701 ttcctgtgtg ctgtacttct ggccggctgc ggacggaaat cggctccgct gtattcattt
     2761 gaggccgaat ccgaggccca ggccgccaag gcatctgggg ccacggtgtc gcgggtggat
     2821 cgcggcgcaa ctgacgggaa gttcgcgctc ttggtagagt tccagcccgg cgatgagccg
     2881 cagatcgagt tcgccgccgg cgagaagctg tgggattgga aggcgaaggg ctcgcttatc
     2941 ctcgacgtca ccaacgcggc cgaagagccg gccgcgttct ccgtggaggt cacggacgca
     3001 gccggcgcga aaacggtcgc aaagacgttc ctgccgttgc gggccggcga gtctgcgagc
     3061 ttcgcgctcc cgttgaactc gccatcgccg ctcgacatgg gcatgcgcgg cgagccggcg
     3121 atcccgggtt tccggctgtt gaatggagac caccagaccg tcgatgtgag ccgaatcgcg
     3181 aagttcagaa tctttgcggc gaagctggaa aagagccgca cgctggtgat cgacaacatc
     3241 cgcctaggac gtggtgtcaa atacgagaag atcgtggacc gctttggcca atttggtctc
     3301 gaagactggc cgggaaagct gaaaagcgag caggagctct ccaagcgccg ctccgaggag
     3361 gaagcggaac tggcagcgcg gcctgtactg ccggaccggg acgagtacgg cggctggtcg
     3421 gccggaccgc aacttgcagc ggctggatat ttccggacgg cgaagcggga cggcaagtgg
     3481 tggctggtaa cgccaagcgg ccaccttttc ttctctttcg gcttcaactc cgtcaattcc
     3541 gaggaaggcg acacggtggt tgagggccgg gagcacatgt tcgagtggct tccgaagccc
     3601 ggagagccac tctcggagca ttacggcaag aattcgatgt ggatgccggt cggattaaag
     3661 atcaaactcg ccacgggccg aacctaccac tactaccgcg ccaacctcga acggaagtac
     3721 ggcaaggact ggtatgaacg ctggcgtgag atcacactga aacggctgcc ctcgtggggt
     3781 ttcaacacga ttgcaaactg gtccgaccca agagtgtacg aagcgcggcg aatcccttac
     3841 gtggtgacga ttgacatcga cgcaaagatc gcggaggttt caagcggcaa cgactattgg
     3901 cggcgaatga tggatccgtt cgatccggcg ttcgcggctg cggtggaggc aagcctcagg
     3961 gccggcacag agaagcgcag agacgatccg tggtgcgtcg gctattttgt ggacaacgag
     4021 ctgtcctggg gatcgatgcg ggacgatcgg ggccgctacg ggctggcgct gggagcgctt
     4081 gcgctcgacg cctcgtcgcc ggcgaagcag gcgttcgtgg agcaactgcg gaaacggcgg
     4141 agcgctatcg ggacgctgaa ccgcgcttgg ggaacgactt tcgcttcttg ggacgcggtg
     4201 ctcggcgagc cgtatcagcc gcagggggaa ctcagcgcgg gcatgaagga cgatctccgc
     4261 gcgtttgtga aggagttcgc tcgcaagtac ttccggacca tccgcgactc cctgaagaag
     4321 cacgacccga accacctgta tctcggaacg cggtttgcgt ggtccagcca ggagagtatc
     4381 gaggcatgcg ccgagatctg cgatgtgatg agtttcaaca tctaccgccc gcgggtggag
     4441 cggagccagt gggtctcatt ggagaaactc ggcaagcccg tgatcatcgg cgagtttcac
     4501 atgggggcgg tggaccgggg gatgtttcat ccggggctgg tgcggaccga agaccaagct
     4561 gcgagggcgg cgctgttctc cgcgtacgtg cgcgacgtga tcgacaatcc gatgttcgtg
     4621 ggctgccact acttcaagta cgttgacgaa ccgttgaccg ggcggccggg cgacggtgag
     4681 aactacgcaa tcgggttcgt caacgtcgta gacggcctac acacggagat gatcgaggcg
     4741 tcgagaacgc ttagcgggga gatgtacaag cggagagcgg gccagtagtt acggtgtcgc
     4801 ctggaagcgt tctttccagg cggcgccacg gccgctccaa taacgcacgg ccgaggcaac
     4861 cgtcgcaccg gtgtagaaga gcgctaccaa agggagcacg ggcgcccaaa gaacgaagcg
     4921 cccgtaaaac cgcagtgacg gcgcatacgt gacggacatc agcagccacg cgagtccgcc
     4981 gagaatagcc gccgggggct tggcagtgag ggcaagcagc ggcggcagaa ggtatgtgac
     5041 gaacatccct gcaaccgtcc cggccaacag cagcgacgag tagctgagtt gtgtgtaagc
     5101 ggtccgcgag atcattcctg cgacggccgt gaaggtcccg taacctctaa tgctgtgcga
     5161 ggccgaggta aggccaagcc agatctttcc tcccgagcgc ttcacctcgc gggcgagcgc
     5221 gcaatcgtcg attacttcgc tacgaatcgc ctctatgcca ccggctcgct cgagcgcttc
     5281 ccggcgcgcc aggagacatc cgcccgcggc gccggcagcg gtgcgaccgc ggtcgctaat
     5341 ccagaccggc gggtagagtt tgaagaagaa gaacaggaac gccggaacgg cggccttttc
     5401 ggcgaacgtc tcacagcgaa gcttgaccat cagggagaca aggtcccaac cggactccgc
     5461 cttggcgacc agtttcgcga gcgtgcaggg cgagtggacg acgtcggcgt cggtgaacca
     5521 gtacgagtcg ggctggaagg attcggcgcg gcggatcccc tcggagagcg cccagagttt
     5581 tccggtccag ccgggaggaa gcggcggggc ggggacgacc tccaacaggc cggacttgcc
     5641 ggcgtcacga gcggcgcctc gggcgacatg ggcagtgtcg tcggtgctgt ggtcgtccac
     5701 gagaaaaacc tggaagagcc ccggatagtc ctgcagcagc accgaggtga ccgcggcggc
     5761 aacggagacg gcttcattgc gcgcggggat aatgacggcg acgcggggcg gggaattgga
     5821 cggacaggcg ccgaggcgct cctcacgcat gcgccagaag cctccgcggg cgagcagaag
     5881 gtagaaccag atcgaaagtg tgaacgcggc gacgagaaac accaggtcac cagacccggc
     5941 aggcattctc ccgcaccatg gggtcgccgg gcttgcagcc gaaggcgtcg cggaactcgg
     6001 gcgagttcgc caccacgccg atgacgcgat agcggccagg cgaatgcggg tccgtcagcg
     6061 cgcgcaggcg cgagatctga tccgttgcgt tctgacacca cacctgcgcg tagccgatga
     6121 agaagcgctg ttcggacgtg aagccgtcga tggtcttctt gcctccctca tcggtaagcg
     6181 tgtccatgag cgccgaatgc gcgagtcgca ggccgccgtt gtccgcgacg ttttctccga
     6241 gggtcagctt tccgttgaga cggacttcgc ccttgtcgtc cttgacggcg acgtattgag
     6301 aatactgctc atcgacacag gcggcgcgct tgtcgaattc cgtcgcgtcc tgttccgtcc
     6361 accagtcggt gagattgccg gacgcgtcga atttgcggcc ctggtcgtcg aagccatggg
     6421 tgagctcatg gccgatgacg aggccgatgg caccgaagtt caccgggtcg tcgatggtcc
     6481 ggctgaagaa gggaggctga aggatacctg cgggaaagtt gatgtcgtta cgcgaaccgc
     6541 ggtagtaggc attaaccgtt tgtggggtca tactccactc ggtacggtcg acgggcccac
     6601 cgattttggc gacctgccgg cgctcttcga aggccgcggc gcgcacgacg ttgcctgcga
     6661 ggtcgtcgcg agcgatgcgg atggagctgt aatcgcgcca cctgtcggga tagccgatgt
     6721 tgttgacgat ggcggcgagc ttctgccggg ccttttcttt cgtggcggcg ctcatccagt
     6781 cgatctgcgc gatgtctttc tccatggcgc tttcgattgc cttcaccatg cggagggtgc
     6841 gctccttgcc gtcggcgccg aagtgcttct ctacgtaaat gcggccgagc gcttcaccga
     6901 gcgatacatc cgcgagacgg acgcagcgtt tccagcgggg agcgatctct ttctgcccga
     6961 cgaggtactg gttgttgaag cgaaacgact cgttcacgaa cagtgaggac agatagtccg
     7021 ctcggctgct tagcacctgc cagctcagat aagtcttcca atccgcgaca ggcacggact
     7081 caagttgcgc ggatacctgt ttgaagaact cagggttggc aacgtttagg gagtcgaatt
     7141 tcggcgcacg ggaggcttcg aagaaacgcg ggaagtcgaa gttcggcgcg agtttcgcca
     7201 gctcggagag cttcatgaag tggtcgcggt tcttcgggtc gcggcggagg gtgcggtcca
     7261 tggaggcgcg agcgagtgcg gtttcgacgg cgaggaccgt ttttgcggct gcggccgagt
     7321 cttcctgcga acggccgagc aggccgaaca tcctgctcac gtgttcgagg aatttctcgc
     7381 gtgtttcctt cgatcggacg tcgtccttga tgtagtagtc gcggtctgga agactgatac
     7441 cgccctggtc cacaacggcg accatgcggg tggcgtcgtg caagtcggcg gtggatcccc
     7501 agccgagcaa ggcgggaacg cccacggtgt gcaaccgcgc cactgcgcgc atcaggtcgc
     7561 ggcgattgcg gatttttgcg acggcgtcga ggtccgcctg gatgggacgc gcgccgagag
     7621 actcgattcg cggctcatcc atgcaggcag catagaaatc gccgatctgg cgttcgggac
     7681 ccggcgcccc ggcgtccgcg gccttctcga gaatctcgcg aagcagcgat aggttgcggt
     7741 cggcgagctc gtcgaagcgg ccccagcggg ccttgtcggg aggaatgggg ttgttctttc
     7801 gccagttgcc gcaagcgaat tcatagaaat cagtgcaggc attgacgccg cggtcgattg
     7861 cgtcgaggtc aaaactgacg atctccttcg gctgcaccga agaggatgac tgcgggcaca
     7921 cgggagaagc ggcaagaaac agaagtaacg accagattgt caggcgcatg gaaaccacga
     7981 ctataacact ggtagcgaga gccgcgaatc aacaccgatg ttccgaagtt tcactcgacc
     8041 agggcggtga cgtagttgat gatcggtggc gactcgccat tcagggtcaa tctcccggta
     8101 ccctggagtt ccaaagcacc actcacaatt cgtagtggtc cgtcgtagtc tccggcagct
     8161 tgcacaacgg tccatgcacc cctcggctgg tagatcaatc ccttgaattt ggcgaatgga
     8221 gtggcccaaa tctggagctg tggcgaatca ctcggcggcg tggcaccacc cgcacacgga
     8281 ctgtcaagac cgccacaact ctcatccacg tagcctgccg aggtgtagcc cacgtaggag
//
LOCUS       DATIFQ010000290        22686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2010598, whole genome shotgun sequence.
ACCESSION   DATIFQ010000290 DATIFQ010000000
VERSION     DATIFQ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 22686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22686
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010598"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VLE22_13575"
     CDS             <1..244
                     /locus_tag="VLE22_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15481.1"
                     /translation="NFEVWREGDVVHVRYGAVTKQCYCPAARYHPVKPRDIHCECTRA
                     THQTIFETALGRPIRVEIVESLRRGGPTCHFVAHVA"
     gene            301..2715
                     /locus_tag="VLE22_13580"
     CDS             301..2715
                     /locus_tag="VLE22_13580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB15482.1"
                     /translation="MLNVLPDLRFAFRSIRKSPGFAAVAVLTLALGVGCNTAVFSLVN
                     AVLLRPMPYFDPGRLVLVWESAPFFGLSDSPVAPANYADWKARSRSFEEMGALEDHSY
                     RLVGEGTPEIVQGSQVTASLLRALRVHPVRGRLFRDDEDRPGAPKVALISEGFWRRRF
                     DADPRITGSTLTLNDEKHVIVGVLPSGKEPPSEYSGTMGEIWTPLGSGYSAQELANRG
                     RHNWMVVARLRSGVSLAEADAEMKTIGVGLAREYPATNEKVGAFVAPMRDHFVSSRRR
                     VLLLLLGTVAFVLLIACANVANLLLARATARTKEVAVRAALGAGAWQLIRQFLCESLL
                     LCAAATAIGLAVATATFRFLAHFTPSDIAGLNALSIDWRVLVFALAISAATLVVFGLV
                     PLHQVRRLDLNQSLKDSARSLAAGSGSRGLRGLLVCSEVALAFMLLIGAGLLIQTFMR
                     LRSVDLGCRTNNILTLQIPPSAAYRDPGRRIAYQGEILRRITAIPGVISAGFTNHIPL
                     VVKGDISGIGAEGRGAKERFQCRTRVAGPGYLRTMGIPLHRGRDIDERDIEGAPLVVL
                     INQTLARTLWPNQDPIGRRIHFTSEVSASVIGVTGDIRQAGLDVPPQPEFYVSALQAG
                     FPVGSLAIHSSVEPATLAAQVRRTIWSVDPNQPITELASMQDILDKEVFQRRVQTMLL
                     AFFAGLALLLAAIGVYGVLAHLVGQQTSEIGLRMALGATPSDVAFGVVVHGLRLTAIG
                     LAFGVAGALAVSRLLTTLLFGVKPTDPATYAIVAAALLLTAAIASYLPAHRAMRVDPI
                     VALSRQ"
     gene            complement(2753..3976)
                     /locus_tag="VLE22_13585"
     CDS             complement(2753..3976)
                     /locus_tag="VLE22_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15483.1"
                     /translation="MNSRRTFVKKMTTVAGGMALSASRVLGANDRIRLGMIGPGFRGL
                     QLIWSMRDPNAEFVAFADAWSGRLEPAKEMAKSPQAKAYMDYRLLLEDRSIDAVIIAT
                     PQHLHCEQFVASLQAGKHVYQEKTMAFSVAHAKRMRAAHRNAPKLTVQIGHQDCSSGQ
                     VADALNILRTGGAGKITEIHSHMYRNTPHGKPQWTRPIPPDMTPEHVAWKAFLGENPD
                     RPFDANRFLNWRFFWDYSGGNFYENMSHQLCFWYKVLNLETPSKVCANGGVFLWKDGR
                     EVPDTMNAAMVHPGDLMFTWDSGFGNDELRVSEDVLGTDGTIFRGEQVRYRPQRVNRP
                     EGTPILGQAIDKGQQHLDIAHVQNFLDCIRSGKEPSCPLDLAFRVSMACRMAVDSYRF
                     GRVLRWDPVKEEIVA"
     gene            complement(4155..7766)
                     /locus_tag="VLE22_13590"
     CDS             complement(4155..7766)
                     /locus_tag="VLE22_13590"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15484.1"
                     /translation="MRHLARIVLAGVSLLALPGLTPNAAHAQESRATITGTVTDPSAA
                     VIPGATIEVTNIATGVVARTVANESGAFRIPYLIPGRYNVTASNAGFKTYARSDLELR
                     VAETVELNIRLQLGGTNETIQVSASAEVLQATEASQSATISKAELDEIPIQGGSALEL
                     LTFAPGMSKQGELRTPYPAWNNGLTMVSSNGAGLAHNSITLDGVANESVGGPGQNGAA
                     NGYTRPAISLSSYAVEEMKIQTNVYDATQGHTSGAVFNMVSRSGTNALHGEAHYQFYP
                     SSIAAQNPFNRSYVYYNSAEQYRYGFSLGGPVVLPKLYDGHNKTFWFFTMERHPFRTP
                     SSSQDSVPTEAQRNGDFSALLALGGQYQIYDPSSIREHADLSRSPFPDNKIPASRISP
                     VATNILKYISSPNNSAGVSPDGQGNYIWVDPQVDTYDTYSSRVDHSFSDRHRLMGRFS
                     WDKWFEGWGDTYGNGTAGERQFRSSYVGAIDDVFTFSPSLVLDVKLGLTYQKYDDGPK
                     TSGVDYAALGFAPQMTGLITKSSAIFPNIGFGDVFDNIGGTAGYYDNNTNETLNATLG
                     WQKGKHSVRFGSEIRNWMQNHLSTSGVNAPIISFSSEYTSGPLPSSNSAPVGQGFAAF
                     LLGVPSNATMRVASPFSANYKWGALFIQDDWRVTPKLTLNLGFRWELELPTTERYNRM
                     EIGFDPSSAQSFGASAQAAYAAAGYAANAANIAAAANLSPAQTATLTDWLAAFPSAYN
                     VRGGYIYASPGNRGTWKTYWKEFLPRAGLAYQLDNKTVIRGGAGIFYDSLGVGRNFLP
                     IQDGFTRDTSIASSVDGGATYANSLVNPFPNGLLPAVGSSLGADLSAGNTIFVGYLNP
                     KEPYSVHWSFGIQRELPGKVLLDASYVGAKGVNLPVTNGCYISGVPCTNLNLVPQRYL
                     SNSPVYDGDNLAVLNARVPNPYAGMTEKLPNLSGATTTVRQLLQPYTQFGAIQATATT
                     GSSMFHSLQARVERRFHRGFSFSGNFTWQRQMDSTNYLNGGDAHPAKMINGADPGLVF
                     NAITVYELPFGKGRLLGGSWHGLLNYIFGEWQVSGTLKAQQGYPASLTDLLLLPGKTL
                     RDLNGERDANNFFNVSALNTDTALQPGSNHLRTLSQQVSFLRGPAMWVTDGAISKKVT
                     IRERVKGEFRFESYNATNHPNLWPWMTINSANSYGAQYNRRYNGLPRTFEFSLRVMF"
     gene            8106..9422
                     /locus_tag="VLE22_13595"
     CDS             8106..9422
                     /locus_tag="VLE22_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB15485.1"
                     /translation="MKEADPSERPGLVTGPQPDARDLLVERVAAGSAFGKSNRLRELF
                     LFLCNRKSTDPGGAVREQEIGIQVFGRRPDYDTSQDAIVRVQVSQLRKRLEQYFASEG
                     RDEPVVIEIPKGGYTPVFRSREPDSSPDRPSELLRAHVPRLGKRWIVVLSVLAAAGVL
                     VVAGLVFGPWHLGSSARWARNAGPGATVDRLWYQIFDNGRPTCIVISDAMLGLFEDAI
                     QRQLSLNEYRDKVFSRLSDERLKDPAEKARWREQVGAYFTHISDARSAAKFSVLNAAR
                     NLSTEIVFAGDFAVSYLQSHNLILVGTRRTNPWVDMFEDQLNFRSVFRETPPMLSYFQ
                     NRSPRPGESAIYAVQWTKQGYCRVAFLPSPGRSGNALLISGTDMASSEAGGQFISSEH
                     WIQTLCSSLGLDRNARFPYFEVLLKVDYMTWNTPKIELVAHRTFKF"
     gene            9574..10734
                     /locus_tag="VLE22_13600"
     CDS             9574..10734
                     /locus_tag="VLE22_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15486.1"
                     /translation="MWTRRDVFASFLTRVGASAICARSGFCAKQETIFENKMISSGNN
                     YPSLNIRANQLTEICVALHRRKAVASIERDLGISPAELRNRIDSLVTAGLVKRSPSGQ
                     FLPTFMVVTLEDAKWMAPPEDMVQATGRLIADRLPDIRARAESIPTIRRAGFSQNAFL
                     ILSNVLLDNWQIENVETEFLKAERPLRNGSRYYYAIFEKPANQRSEAFGIYGNGGGTM
                     GKVRLGVYGKRRYDGSTLLFPSKEEFARLFELPAQADVKAEAGALLEHMVAFARTGED
                     HLSATQKAGLGKLGLFENGELRVTVFSGAEEKELGDVAALLTPSLLGLLKRRRAGLQS
                     AHGRSPYREEVTFNEFFIWWYHFFYSATTDWLAHKGLLQIPRSGNTTYLIAD"
     gene            complement(10794..13055)
                     /locus_tag="VLE22_13605"
     CDS             complement(10794..13055)
                     /locus_tag="VLE22_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB15487.1"
                     /translation="MPSVKFGVFELDLCARELRKRGIRLRVPYQSLEVLALLVQRPGQ
                     LISREEIRQQLWPDGTVVEFEHGVNNAVMRLRDALSDSADTPRFVETLPRRGYRFVAP
                     VEAIQPEAPLSSQRIPTEAARGAIADVTPSEQPKEEAPPTAQLPPARHHRTVWIIRAL
                     GVAVVVLAILSVVLYSTRSEPVEISNIRFTPFATDPELEVMGAWSPDGKSVAYLKEIN
                     GRVQVMVRGLDAATPAQLTSLPEGVAATNVISPVFWSPEGDRIYFMSGNALWSIALAG
                     GQPQRILEEEVRAAAISPDGKTLAIWRRIEEGGRVYTSVWISKPPGARPRKYTPAPFQ
                     MVGAFMPVFLRFSPDGTRIALSGWRTGIGDSATWILPWPDGTDATPRQLSPQRRMRVP
                     QFDWMPDSRHLVSTGVTTPSAALYFWDVERDRMHQLTTPAFTGSIQYPSVSPDGRRVM
                     FSAVRTDFDIVRVPLDGSPFRPALSTSMDEYSPSSSGDKMAFVTDRSGQSEIWLRSMT
                     AGWERPLVRQSDFPPGSPVPFSDVALSPDGSRVAYRRGARLWISPAAGGAPSLAIPVG
                     DPQYTAASWSPDSSSLVCQVSPSGGKVVAVARVGSREPPRILAETLMVCNTSPLWSPD
                     GRWIACGHRDGMLLLVSPDGSHRRTIPSPEWPTSRGFVLVWSHDGSTLYEASSRSGQK
                     AWLDAIDVETGKSHRVAEYDEFVRFDSPVNYSLSGSLAPDGKSFFTTAASFKSDLWIL
                     DGFPQPQRKWFWW"
     gene            13237..16917
                     /locus_tag="VLE22_13610"
     CDS             13237..16917
                     /locus_tag="VLE22_13610"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15488.1"
                     /translation="MSKHCVLALLLIALLGLPAWPQASTGAVSGTVRDLTEAVVPSVT
                     VTLVNTATGGASRTVTNEVGLYTFPGVVPGPYRLSIAHEGFERFEVALTVHVGENLAV
                     DPLLKPGQTATTVEVRDVTPIVKLDSPTLGHVLERQRIEQLPLNGRTITTLLNTVPGM
                     EGNRAYGLREGSQDMILDGATLTDRNWGGNPRRQPGLDSIQEFNAEDSGASAKFSRPT
                     PILYSTRSGTNDFHGSLFEFNRNNGYGKARRREEGNKPSFLNRNEFGGTAGGPVWLPK
                     LYDGRNRTFWFFSHEAHRNIAPYSAEFEVPTEAMRNGDFSQLYDDAGRFIKIYNPWTT
                     DPNTWSREQFSYQGRANVIDPALMSPLAKKLFDITPAPTHPEVNPLVDSNWIGPVPDT
                     TRQWTLTTRLDHRFSDNDQVYVRYTQGSHHQLSQFYGQPMLNNVAGLRNVTAPNRSAA
                     VSWVRTLSPTWFNELLVSFNRENAFSGTGEPGVRYAAELGLPNPFNREGWPAIYETGL
                     SNYYFETENTKRTPFTYALIDDNVTKVIGRHELQLGGHYRFDQLNYMYEQQYTQGEHQ
                     FDTLSTALYDPSSPRSEPYEVQNTGQYLANMFIGSTNYSANFDRGYFYARSKEYAFYL
                     QDRFRVTPRLTLSLGLRWEYWPPYREKYNMLVAYDPDRKAAVLGSDLESMYRVQAALP
                     SIVDRYQQLGLQFLSYQEAGWPERLMDSTRKNFGPRLGFAYRLTNGDRAAVLRGGYRV
                     SYFPIPMSTWGQRQRMNVPSHARFTNYSAIDSWESPDGISNYSLRSAPTIIAGKNSID
                     AIPTNDASFIARGQGSLSYFAKEQPDTRVQDWNLTLEKEVLPDTVARVAAVGNHTSNL
                     EQYYRYNENPPAYVWYARTGLPRPSGEFGNVAMRPHDQQVLGTIEEFQKSGWSNYSGV
                     QFELERRFNKGYGFQVFYNIHNVLGAGGGGYGVYILDPTQFLPGAVPEDMHDRARFLN
                     YRRERDYPKHSVRWNWVAELPFGKGKPFGGNASGALEKLIGGWQISGMGYLRSTYFEL
                     PTNLYPTTGNPVEIYGYKYPIQDCRSGTCRSGYLWWNGYIPPHKINSYDANGQPNGVM
                     GVPESYRPAGQPLNPWPANPDPADPMYDSYGTNRVWVTLNDGSRLQTDYNDNLHPWRN
                     QYFPSVRQWSMDAQLSKRIPITERVALQIKVDAFNVFNAPNNFFRGCATCSDGIISTL
                     SSAPGYANFYPREMQLGARLTW"
     gene            17053..17829
                     /locus_tag="VLE22_13615"
     CDS             17053..17829
                     /locus_tag="VLE22_13615"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB15489.1"
                     /translation="MTHEFDGKKYERASTHQKEWGAKLIVELSLQGTERVLDLGCGDG
                     TLTAQLADLVPSGEVIGIDASHGMIDAAQRKSRRNLTFLVTDINDMDFVEQFDVVFSN
                     ATLHWVKDHGILLRNVNRALCVGGRLRFNFAGEGNCPHFYKVIREAMLLEAFADYFSE
                     FVCPWYMPSVDEYSAVVKASGLGGARVWGENADRFFQNAEQMTNWIDQPSLVPFLVRV
                     SDRKKGAFREYVVRRMIEETQQDDGRCFETFRRINVYATT"
     gene            complement(17925..19028)
                     /locus_tag="VLE22_13620"
     CDS             complement(17925..19028)
                     /locus_tag="VLE22_13620"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB15490.1"
                     /translation="MERRQFINRALMATGLPVARGLAERSPKKKLGIPGPYPGRVVAV
                     RHPASVISGTYQREPVRAMMRRGMMELTGAPAWMDAWRLFFEPGDVVIIKVNPVGQPY
                     LISAPEVFQEIVAGLEEAGVKRNDIIAYDRYYDQFLGGGFDKWLPEGVRWSYATKKVA
                     PFQLDMEGYDPDHYMEMALVEAKANPTNPHHRRSYVARLLTKEANKLINLGVVKHHQS
                     AGVTIALKNLSHGLVNNVARSHVSSSNNSCGMFIPTVIDIPVIKQKTVLNIIDGVKAA
                     YHGGPGSRVGKYLWEHKTLYFATDPVAVDTIGLGVIDDKRVQAGMQTIALAPADEDSV
                     FVRMQPEHIELAGLLGLGVSDPDKIDLKTVSLG"
     gene            19160..21016
                     /locus_tag="VLE22_13625"
     CDS             19160..21016
                     /locus_tag="VLE22_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB15491.1"
                     /translation="MDQVGGYMGNPGNSLPDAQSDIVGTPLQEPNGGKRIVLSAPLTE
                     TVDHAGYFIQMALASLPARLEGIINSRYPRWRDLERNSDGSARWMPAGVRLLEAALLR
                     QYPREELVACYPDDLERFIGPKTKLVAVSTHNPLGVTFAAGVYTSVFGSSREPINSHY
                     AKQLFATIKANPNRANFKVIVGGSGGWQIIQTDTWDELGVDCVVEGRSESEDTMELIR
                     RAIRGEPLPRKLEVAHPKDRDSILVPENRTTFGVVEMTTGCGRRCNFCVPDLNPQLDV
                     AKEKIMRGVLTNVRQGNTQISLATEDMFIWGQVRTGVPFYFPNREALLDLYSEIVGTP
                     GVEKHLLSHATIAPSVVDPLLIEKLSDVLLDKSPIHLPTHSSHPRKKILSPLIGLETG
                     SVRLARAIMPGKAVPFSIDDWPSVVVQGLTVLNRNNWFPVMTLMIGNPGETDEDVKAT
                     LDLVYEIERRGLFAFLVPSIFTPLHDTRMEKKKGVTETRNLSPLQWQLMMKCWKMNLR
                     PGQYSWWGPTAWRLGAIGLWLYKLRRVNGPNFTWPLLMFASAVPERVMERMGKIYIGK
                     PIAIKSRKELLATVKREHWVHLRRDTGDLPDDYEASVTVGRASSAFRVTSAS"
     gene            complement(21021..21239)
                     /locus_tag="VLE22_13630"
     CDS             complement(21021..21239)
                     /locus_tag="VLE22_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15492.1"
                     /translation="MESQIDSHLDGRQHGGERKLTPEQFEHRREKANLLLSRTRVLHD
                     LEECRNPRYRKILQDSLAFLDVKLAELK"
     gene            21354..>22686
                     /locus_tag="VLE22_13635"
     CDS             21354..>22686
                     /locus_tag="VLE22_13635"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15493.1"
                     /translation="MSSSRRDFVKTAALAAAPAVRPAWGQSSPNNRINIGVVGFHGRG
                     RDHYREFARMPNVKVAYLCDIDERLYPGAVAEIEKMAGNKPETFVDLRKMLEKKDLDA
                     ISIATPDHWHALQMIWACQAGKDVYVEKPVCHNLREGRKMVEAARKYNRIVQCGLNRR
                     SEARTRTGVRFVQEAKFGSAYRAKAVVYRGRTSIGRVQESSIPNGVHWDLFLGPAPYR
                     AFTLNRFHYGWHYYWDTATTEVGNNGVHAIDVVRWATNRNVHPVKIHCAGGLFADNSE
                     QETPNVQNASYEYADGTLLELEVTTLPSPTFGGVHMGEFFYTPQGYVTSANKWSTVVG
                     EFTVRNRPNPPSGVDERMGNISFPNIAYKPGPVIPESTETEVSHFQNFIDCVRSRKRE
                     DLHCEMIEGHMSTSLCHLANIAFRTGRKLTFDPAAETFPGDAEANALLTRKY"
BASE COUNT         4465 a         6947 c         6863 g         4411 t
ORIGIN      
        1 gaacttcgag gtctggcgcg aaggggacgt ggttcacgtc cgctacggcg cagttaccaa
       61 gcagtgctac tgcccggctg cccggtacca tccggtaaag ccccgcgaca tccactgcga
      121 atgcacccga gccacccacc agacgatctt cgagacggcc ctgggccggc ccatccgggt
      181 cgagatcgtg gagtccctgc ggcggggcgg cccgacgtgc cacttcgtgg cccacgtggc
      241 ttgagatcgc cccggcgact cggagcgaac aaatttaata cctgcgcgtc tatatattcg
      301 atgctcaatg tcctgccaga tctccgcttt gccttccgct cgatcaggaa gtcgcccgga
      361 ttcgccgcag tcgccgttct gaccctcgct ctcggcgtcg gatgcaacac ggctgtcttc
      421 agcctcgtca acgccgtcct gctgcggcca atgccgtatt tcgaccccgg ccggctcgtt
      481 ctggtctggg aaagcgcacc gtttttcggc ctgagcgaca gccccgttgc ccccgcgaac
      541 tacgcggact ggaaagctcg cagccggtcg ttcgaggaaa tgggagcgct ggaagaccac
      601 tcgtaccgcc tcgtcggcga gggtacgccg gagatcgtac agggcagcca ggtaacagcc
      661 agcctgctac gggcgctgcg cgtccatccc gtgcgcggcc gtttgttccg cgacgacgag
      721 gaccggcccg gcgccccgaa ggtcgccctc atcagcgagg gtttttggcg ccgccgcttt
      781 gatgcggacc ccaggattac cggcagtacc ctcaccctca atgacgagaa gcacgtgatc
      841 gtcggtgtcc tgccgtccgg caaggaaccg ccctcggagt acagcggcac gatgggcgag
      901 atctggaccc cgctcggcag cggatactcc gcacaggaac ttgcgaaccg cggccggcac
      961 aactggatgg ttgtcgcgcg gctgcgatcc ggagtctccc tcgcggaagc tgacgcggag
     1021 atgaagacga tcggcgtggg tctggcccgc gagtatcccg cgacgaacga gaaagtcggc
     1081 gcgttcgtgg ctccgatgcg cgaccacttc gtcagctcgc gacgccgcgt gctcctcttg
     1141 ctgctgggta ccgtggcatt cgttctcctg atcgcctgcg cgaacgtcgc gaacctgctg
     1201 ctcgcgagag cgacggcgcg gacgaaggaa gtcgccgtac gcgccgcgct gggcgccgga
     1261 gcgtggcagc ttatacgcca gttcctgtgc gagagcctgc tgctctgcgc cgccgcgacc
     1321 gcaatcggcc ttgccgtcgc aaccgcaacg ttccgctttc tcgcgcactt caccccgagc
     1381 gatatcgcgg gtcttaacgc gctctccatc gactggcgcg tcctcgtctt cgccctcgcg
     1441 atctccgctg cgaccctggt ggtcttcgga ctggttccgc tgcatcaggt ccgccgcctg
     1501 gatctcaacc agtcactcaa agatagcgcc cgcagcctgg cagcgggctc cggatcgcgc
     1561 ggtctgcgag gactgctcgt ctgctccgag gtggcactcg ccttcatgct gctgatcggc
     1621 gcaggcctgc tgatccagac tttcatgcgg ctgcggagcg tggacctcgg ctgccggacg
     1681 aataacatcc tcacgctgca aatcccgccg tcggcggcgt accgtgatcc cggccgcagg
     1741 attgcctacc agggggaaat cctccgccgg atcacagcga tccccggagt gatctccgcg
     1801 ggcttcacta accacatccc gcttgtcgtc aagggagaca tcagcggcat cggcgccgaa
     1861 gggcgcggcg caaaggagag gtttcagtgc cgcactcgcg tggccggccc ggggtacctc
     1921 cgaacgatgg gcatcccgct gcaccgcggc cgcgacatcg acgagcgcga catcgagggc
     1981 gcgcccctgg tcgttctgat caaccagacc ctcgcccgca ccctctggcc caaccaggac
     2041 ccaatcggcc ggcgaatcca cttcacctcc gaggtgtccg cgtcagtgat tggcgtgacc
     2101 ggcgacattc gccaggccgg cctggatgtg ccgcctcagc cggaatttta cgtctccgcg
     2161 ctgcaggccg gttttcccgt gggctcgctc gcaattcaca gcagcgtcga acccgccacc
     2221 ctggccgcgc aggtccggcg gaccatctgg tcggtagacc cgaatcagcc cattacagaa
     2281 ctggccagca tgcaagacat cctggacaaa gaggtcttcc agcgtcgcgt ccaaaccatg
     2341 ctccttgcct tcttcgccgg cctggctctc cttctcgctg ccatcggagt ctatggtgtg
     2401 ctggcgcacc ttgtcggcca gcagacttcg gagatcggcc tccgcatggc tttgggcgcc
     2461 actccctccg atgtcgcttt cggcgtcgtc gtgcatggcc tcaggcttac cgccatcggc
     2521 ctcgcgttcg gagtcgcagg agccttggcg gtctcgcgcc tgctcaccac gcttctattc
     2581 ggcgtcaagc cgaccgaccc cgccacatac gcaatcgtcg ccgccgcgct cctgctcacc
     2641 gccgcgatcg ccagctacct gcccgcgcac cgggccatgc gggtggaccc gatcgtggcg
     2701 cttagccggc aatgaggccg atggacggtc gagccactac cgtgtaaatc gctcaggcga
     2761 cgatctcttc tttcacagga tcccagcgca gcacgcgtcc gaaacggtaa ctgtccaccg
     2821 ccatgcggca ggccatcgag acccggaagg ccaggtcaag cggacagctc ggctccttgc
     2881 cggaacggat gcaatccagg aagttctgta cgtgcgcaat gtcgagatgc tgctgtccct
     2941 tatcgatcgc ctgtcccagg atcggggttc cctcgggccg gttgaccctc tgcggccggt
     3001 atcgaacctg ttcgccgcgg aagatggttc cgtcggtgcc cagcacgtct tcggacacac
     3061 gcagttcgtc gtttccgaaa cccgaatccc aggtgaacat gagatctccc gggtgaacca
     3121 tcgccgcgtt catggtgtcc ggcacttccc gtccgtcctt ccagaggaac actccgccat
     3181 tcgcgcagac cttggaagga gtttcgagat tcagcacctt gtaccagaaa cacaactggt
     3241 gtgacatgtt ctcgtagaag ttacccccgg agtagtccca gaaaaacctc cagttcagga
     3301 aacgattggc gtcaaagggg cggtccgggt tttccccgag gaaagccttc caggcgacgt
     3361 gctcgggcgt catgtccgga ggaatggggc gagtccactg cggcttgcca tgcggcgtat
     3421 tgcggtacat gtgcgaatgg atttcggtga tcttgccggc gccacccgtc cgcaggatat
     3481 tcagggcatc ggcgacctgg cccgaggagc agtcctggtg gccgatctgg acggtcaact
     3541 tgggggcatt gcgatgggcg gcgcgcattc gcttggcgtg ggccacgctg aaggccatgg
     3601 tcttttcctg atagacatgc ttgccggcct ggagcgaagc cacgaattgc tcgcagtgca
     3661 ggtgctgggg cgtagcgatg atgacggcgt cgatggaccg gtcttccagc agaaggcggt
     3721 agtccatgta ggccttcgct tgcggagact tggccatttc ctttgccggc tccagccggc
     3781 cggaccaggc gtctgcaaat gccacaaact cggcattggg atcccgcatc gaccagatca
     3841 actggagtcc ccggaaaccg gggccgatca tgcccaggcg gatgcggtcg ttggcgccga
     3901 gaactcgcga agcagagagc gccatccctc cggcgacggt ggtcatcttc ttgacaaatg
     3961 tgcggcgtga attcatgatt gctcctttcc gttcggcacc gcttgcttac gctcgcggtt
     4021 cactaacacc ttgactgtca acacgacgat ttcctgaacc tcacccgtca gggatctcgg
     4081 acggctgctc aagccggcct cgcagccggc ttgagctctg cgcagcccca ctctcggggg
     4141 actggagggt ggggttagaa catgacccta agactgaatt cgaaagtgcg cggcagtccg
     4201 ttatatctgc ggttgtactg cgcaccgtaa ctgttggcgg aattgatcgt catccaaggc
     4261 caaaggttgg ggtggttcgt tgcgttatac gattcaaaac ggaactcccc tttgactctc
     4321 tctcggatcg tgaccttctt gctgatcgcc ccgtccgtta cccacatggc gggcccgcgg
     4381 aggaagctga cttgctgtga aagcgtccgg aggtggttcg agcctggttg caaggcggta
     4441 tccgtgttta gtgcggagac gttgaagaaa ttgtttgcgt cgcgctcgcc gttgaggtcg
     4501 cgcaaagtct tgcccggcaa caggagcagg tcggtaagcg aggccggata gccctgctgg
     4561 gccttgaggg ttcccgagac ctgccactcg ccgaaaatgt agttgagcaa gccgtgccag
     4621 gaaccgccca gcagccggcc ttttccgaag ggcaactcgt agacggtgat ggcgttgaat
     4681 accagcccgg ggtcggcgcc gttgatcatt ttcgccgggt gcgcgtcacc cccgttcagg
     4741 taattggtgg agtccatctg tctctgccag gtaaagttgc ccgagaagct gaacccccgg
     4801 tggaaacggc gctcgacgcg cgcctgcaag gaatggaaca tgctcgagcc ggtcgtcgca
     4861 gtggcctgaa tcgctccgaa ctgcgtgtag ggctgaagca gttgtcgaac ggtggtggtg
     4921 gcgccgctca ggttgggcag tttctcggtc atcccggcat agggattggg cactcgggca
     4981 ttgagcaccg ccaggttgtc tccatcgtac acgggggaat tggacaggta tcgttgtgga
     5041 accaggttga ggttcgtaca tgggacaccc gagatgtaac atccgttggt gaccggcaga
     5101 ttcacgccct tggccccgac atagctggca tcgagaagaa ccttgccggg caactcgcgc
     5161 tggatgccga atgaccagtg caccgaatac ggctccttcg ggttgagata gccaacgaag
     5221 atggtgttgc cggcgcttag gtccgcgcca agcgagcttc caactgccgg cagcaggccg
     5281 ttggggaacg gattgaccaa gctgttcgcg taagtagcgc caccgtccac agaagaggca
     5341 atactggtgt cccgggtgaa cccgtcctgg atgggcagga agttgcggcc gactccgagc
     5401 gagtcataga agatgccggc gccgccgcgg atcacggtct tattgtcaag ctggtaggcc
     5461 aggccggcac gaggcaggaa ttccttccag taggtcttcc aggtgccgcg gttgccgggg
     5521 ctggcataga tatatccgcc cctcacgttg tacgcggagg ggaaggccgc cagccagtcc
     5581 gtaagcgtgg ccgtctgggc cgggctcagg ttcgccgccg ccgcgatatt ggccgcattc
     5641 gccgcatagc cggccgcggc ataggccgct tgggccgagg cgccaaagct ctgtgcggag
     5701 ctgggatcga agccgatttc catgcggttg tagcgctccg tggtcggcag ctccaattcc
     5761 caacggaacc cgagattcaa ggtcagcttg ggagttaccc tccaatcatc ctggatgaac
     5821 agagcgcccc atttgtaatt ggccgaaaag gggctggcaa ccctcatcgt ggcgttactc
     5881 ggcactccca ggaggaacgc ggcgaaaccc tggccgacag gagcggaatt ggaggatggc
     5941 agcggaccgc ttgtgtactc gctgctgaac gaaataatgg gggcattcac gcccgaagtg
     6001 ctcaggtggt tctgcatcca attccggatc tcggatccaa agcgcacgct gtgcttgccc
     6061 ttttgccagc ccagggtggc gttgagggtc tcgttggtgt tgttgtcgta gtaacccgca
     6121 gtccctccaa tattgtcaaa aacatcgccg aaaccgatgt tcggaaaaat ggcgctggat
     6181 ttcgtgatca ggccggtcat ttgcggcgca aagcccagag ccgcgtagtc aaccccgctg
     6241 gtcttggggc catcgtcgta tttctggtag gtcaggccca gcttcacatc gagaaccagg
     6301 ctgggagaaa aggtgaagac atcatcgatc gcgcccacat acgagcttcg gaactgcctt
     6361 tctccggctg ttccgttgcc gtaggtgtct ccccacccct cgaaccactt atcccaactg
     6421 aaacgcccca tcaggcggtg cctgtcggaa aagctgtgat ccacgcggct gctgtacgtg
     6481 tcgtaggtgt cgacctgcgg atcgacccag atgtagttgc cttgaccgtc ggggctgact
     6541 ccggccgagt tgttcgggga gcttatgtac ttcaagatgt tcgtggccac cgggctgatc
     6601 ctgctggcag ggatcttgtt atcgggaaat ggagaacggc tcagatcggc gtgctccctg
     6661 atcgaggaag gatcgtagat ctggtattgg ccgcccaagg ccaatagagc agagaaatcg
     6721 ccgtttcttt gcgcctcggt cggcacggaa tcctggctcg acgaaggcgt cctgaagggg
     6781 tgcctctcca tggtgaagaa ccagaaagtc ttgttgtggc cgtcatagag cttcggcagg
     6841 acaaccgggc cgcccagcga gaagccgtac cgatactgtt cggcgctgtt gtaatacacg
     6901 taggagcggt tgaaaggatt ctgcgccgcg atggacgacg ggtagaactg gtagtgcgcc
     6961 tcgccgtgga gggcgttggt gcccgagcgg ctgaccatgt tgaagaccgc gccggaggtg
     7021 tgaccctggg tcgcgtcgta tacgttggtc tggatcttca tttcttcgac tgcatagctg
     7081 gaaaggctga tggccggccg cgtgtatcca ttcgccgctc cgttttgccc gggcccgccc
     7141 acgctctcgt tcgcaacacc atctaacgtg atgctgttgt gggccaggcc ggcgccgttg
     7201 gagctcacca tcgtcaggcc gttattccag gccgggtagg gcgtgcgcaa ctctccctgc
     7261 ttcgacatgc ccggcgcgaa ggtgagaagc tccagagccg agccgccttg gatgggaatt
     7321 tcgtccaatt cggctttact gatggtcgcg ctctggctcg cttcggtcgc ctgcaacacc
     7381 tccgccgaag cgctgacctg gatcgtttca tttgtcccgc ccaactgaag ccggatgttc
     7441 agctcgaccg tttccgccac acgcagttca aggtcggagc gggcgtaggt tttgaacccc
     7501 gcattactgg cggtcacgtt atagcggccg gggatcagat aggggattcg gaaggcgccg
     7561 gactcattgg cgacggtcct ggcgacgacg ccggtcgcaa tattggtgac ttcgattgtc
     7621 gccccaggga tcacagcagc gctggggtcg gtcacggtgc cggttatagt ggcgcgggac
     7681 tcctgtgcgt gagccgcgtt gggtgtcaga ccgggaagcg caagcagcga cactcccgcg
     7741 agcacgattc ttgccaagtg tctcatctga gccgagacct cctgtggaaa atgaacctag
     7801 cccatactca aaggggggag gccccgtgcg caagtcaatt cgcggcgaat tcgccgtgtc
     7861 ttctccgttc gttaacaacg ttcgttaacg gcgtttacgg cctgtttatc atgtgttaga
     7921 cttgtcccaa tcttggtagt gaaccaggac cgagtagggt gcgctggcgc cctggaccga
     7981 gccggggctt tggcccggct cttttcggcc cccggcgggt ggccagagat cggccgcgga
     8041 caagggggtc ccttccacag aatttgcggc attcgtcgtt tggaggcgca aaacaggccg
     8101 gagtcatgaa agaggctgat ccaagtgaac ggccaggcct tgtgaccggc ccccagcctg
     8161 acgcgcgcga ccttctcgtg gaacgcgtgg cggctggttc cgcgtttgga aaatcgaacc
     8221 gtctgcgtga gttgtttcta ttcctctgca atcggaaatc gaccgatcca ggaggcgcgg
     8281 tccgggagca ggagatcgga atccaggttt tcggtcgccg gccggactac gacacgtcgc
     8341 aagacgccat tgttcgtgtg caggtttccc agctccgcaa gaggctagag cagtatttcg
     8401 cctccgaggg ccgggacgag ccggtggtga tcgagattcc caaggggggc tacacgccgg
     8461 tcttccgcag ccgcgagcca gattcctcgc cggatcggcc ctcagagttg cttcgagcgc
     8521 acgtgccacg cctggggaag cggtggattg tagtgctctc ggttttggcc gcggctgggg
     8581 ttctggtggt agcgggactc gtgtttgggc cctggcacct aggcagttcg gcacgctggg
     8641 cccgcaatgc cggtcccggg gcaactgtag atcgtctgtg gtaccagatc ttcgacaacg
     8701 ggcgcccaac ctgcatcgtt atctctgacg cgatgctcgg attgttcgag gacgcgatcc
     8761 agcgtcaact ctcgctgaac gaataccggg acaaagtttt cagccgtctg agcgacgagc
     8821 gactgaagga cccggcggag aaagccaggt ggagggagca ggttggggcg tacttcacgc
     8881 acatttccga cgcccggtcg gcggcgaagt tctcggtgct caacgcggcg cgaaacctgt
     8941 ccacggagat cgtcttcgca ggggactttg cggtgagcta ccttcagtcg cacaacctca
     9001 ttctggtggg cacccggcgg acgaatccct gggtggacat gttcgaggat cagttgaact
     9061 ttcgtagtgt atttcgagaa acgccgccga tgttgtccta ctttcagaat cggtcgcccc
     9121 gcccgggcga gagcgcgatt tatgcggtcc aatggacgaa gcaggggtac tgccgggtgg
     9181 cctttctgcc aagccctggc cgaagcggta atgctctgct gatctcgggc actgacatgg
     9241 cgtccagcga agcaggcggg caattcatca gcagtgagca ctggatccag acgctgtgtt
     9301 catcactggg actagaccgg aatgcgcggt ttccctattt cgaggttctg ctcaaagtcg
     9361 actacatgac ctggaacact ccgaagatcg agctggttgc ccaccgcacc ttcaaattct
     9421 gaattcgtga gtcgatcacc gtccggcatt acgaaaactg ctttcggccg cagggcgccg
     9481 ggcgttccgt aatccacgcc gaatagtgcc gttgccacaa cgggtgtcga aaaactatcg
     9541 aactggggac gtgggcggac cgtataaacg agtatgtgga cacgccggga cgtcttcgcg
     9601 tcttttttga cccgcgttgg ggcgagtgcc atctgcgccc gcagtgggtt ctgtgcaaag
     9661 caggagacca tcttcgaaaa caagatgatc tcatctggca acaactaccc ttctctcaac
     9721 attagagcga accagctcac tgagatctgt gtggctttac accgccggaa ggcagtggca
     9781 tcgatcgagc gcgatctcgg gatctcgccc gcagagcttc gcaacaggat cgattctctg
     9841 gtcacggcgg ggctggtcaa aagatcgcct tccggccagt ttcttccgac gttcatggtc
     9901 gtcacgcttg aggacgccaa atggatggcg ccgccggagg acatggtgca agcgaccggc
     9961 cgtctcatcg ctgacaggct ccccgatatc cgggcgcggg ccgagtcgat cccgacaatc
    10021 cgccgggccg ggttctccca aaatgctttc ctcattttga gcaacgttct cctcgataac
    10081 tggcagattg agaacgtgga aacggagttt ctgaaagcgg aacggccatt gcgtaacgga
    10141 agcaggtact actacgcaat cttcgagaag ccagccaatc aacggagcga agcgttcggc
    10201 atatacggaa acgggggagg cactatgggc aaggttcgtc taggtgtcta cgggaagcgg
    10261 agatacgacg gcagcacatt gcttttcccg tcaaaggaag agttcgcacg gctgttcgag
    10321 ttgccggcgc aggcggatgt caaggccgaa gcgggcgccc tgctggagca catggttgcg
    10381 tttgcgcgga cgggagaaga ccacctgagc gcaacgcaga aggcggggct gggcaagtta
    10441 gggcttttcg aaaacggcga actgcgggtt acggtgttca gtggcgccga ggagaaggaa
    10501 ttgggggacg tggccgcact attgacccct tctttgctgg gcttgctcaa acgccggcgg
    10561 gccggattgc agagcgcgca cgggcgctcg ccgtacaggg aagaggtgac cttcaacgag
    10621 tttttcatct ggtggtatca cttcttctat tcggccacga ctgactggct cgcgcacaaa
    10681 ggcttgcttc agatcccacg ttctggaaac acgacgtacc tgattgcaga ttaacggctc
    10741 ctgtactggt gttacggcga agcgccttct ccattttcag ctgaaccgga acactaccac
    10801 cagaaccact tgcgctgggg ttgcggaaaa ccgtccagaa tccagaggtc agatttgaag
    10861 gatgccgccg tagtgaagaa actcttccca tcgggagcca ggctgccgga tagcgagtaa
    10921 ttcacggggc tatcgaagcg gacgaactca tcgtattcgg cgaccctgtg cgatttcccg
    10981 gtttcgacat ctatggcatc caaccacgcc ttttgccccg accgggagga cgcttcgtaa
    11041 agcgtcgagc catcatgtga ccagacaagc acaaaccctc gcgaggtagg ccactcggga
    11101 gacggtatgg tccggcgatg gcttccatcg ggtgacacga gcagcaacat gccatcgcga
    11161 tgaccgcacg cgatccagcg gccatcgggc gaccagagtg gagacgtgtt gcacaccatc
    11221 aacgtttccg caagaattcg gggcggctcc cggcttccca cccgcgctac cgctacgact
    11281 ttgcctccgc tggggctgac ctggcatacc agcgacgaac tgtcgggtga ccacgaggcc
    11341 gcggtgtact gagggtcgcc aacggggatc gcgagcgacg gcgcgccgcc ggctgccggc
    11401 gaaatccaga gccttgctcc acgccggtac gcgacgcggc tgccgtcggg cgacagggct
    11461 acgtctgaga acggaaccgg actacccggc gggaagtcgc tctgtctcac cagcgggcgt
    11521 tcccaaccgg ccgtcatgct gcgcaaccag atctcggatt ggccggagcg atcggtaacg
    11581 aacgccatct tgtcgccgga cgaggatgga gagtactcgt ccatcgaagt gctgagcgcg
    11641 ggacggaacg gcgagccgtc cagcggcacg cgcacgatgt cgaagtcggt ccgaacggcg
    11701 gagaacatga cgcgacgccc gtcaggcgag acagacgggt actggatcga gccggtgaat
    11761 gccggtgtgg tcaactggtg catgcggtcc ctctcgacgt cccagaagta cagcgcagcc
    11821 gaaggggtgg ttacacctgt cgaaaccaga tgccgtgaat cgggcatcca atcgaactgc
    11881 ggcactctca tccggcgttg cggcgagagc tgcctcggtg tcgcgtcggt tccgtcgggc
    11941 cagggcagaa tccaggttgc ggaatcgcca attccggtcc gccagccgct tagcgcaatt
    12001 cgagttccgt ccggcgaaaa acgcaggaat accggcatga aggcgcccac catctggaag
    12061 ggcgcgggcg tgtacttgcg cggacgggcg cccggcggct tcgaaatcca aaccgaagtg
    12121 taaactcttc ctccttcttc aatacgtctc cagatcgcca gcgtttttcc gtctggtgag
    12181 atcgcggcgg cccgcacttc ctcctcaagg atgcgctgag gctgccctcc ggcgagagcg
    12241 atcgaccaca aagcgttgcc actcatgaaa tagattcgat cgccctcggg tgaccagaac
    12301 accggcgata ttacgtttgt cgctgctacg ccctcaggaa gacttgtcag ttgagcggga
    12361 gttgccgcgt ccaaaccacg caccatgacc tgcacacggc cattgatttc tttcaggtac
    12421 gccacgctct tgccgtctgg cgaccaggct cccatcactt ccaactcagg gtcggtggcg
    12481 aacggagtga acctgatatt cgaaatctcg accggttcgg accgcgtcga gtacaagacg
    12541 accgaaagta tggcgagcac gacgacggca acaccgagcg cccgtatgat ccaaacggtt
    12601 ctgtgatgac gcgcaggcgg gagctgcgca gtcggggggg cctcttcctt tggttgctcg
    12661 gacggggtga catcggcaat agctccgcgc gcggcttcgg tgggaatccg ctggctcgac
    12721 agcggcgctt caggttggat cgcttcgaca ggcgcgacga agcgatatcc acgacgaggc
    12781 agggtttcaa cgaaacgcgg ggtgtcggcc gaatccgaga gggcgtcccg cagtcgcatc
    12841 acggcgttgt tcacgccgtg ctcgaactcc acgacggtcc cgtcgggcca gagttgctgg
    12901 cggatctctt cccggctgat cagttgaccg ggacgctgca caagcagcgc caacacttcg
    12961 agggactgat aggggacgcg aagccggata cctcttttcc gcagctcccg cgcacacaag
    13021 tcgagctcga aaacaccgaa tttgacagat ggcaccggat ccactcagag gatatcatgt
    13081 gccagaaatg ggatctagga caataagatc cttatttttc aattgcttat cttgggacat
    13141 acgcacgagt ggcaatgcat cgaaccgtaa acggaattcg gccaaagtta aggcggtaga
    13201 ttccttttta cctactgatt caggagtcca ataccaatgt cgaaacactg cgtactcgct
    13261 ttgctgctca tagcgctcct tggactgccg gcttggccgc aagccagcac cggggccgtg
    13321 agcggaacgg tccgcgacct gacggaagcc gtggtcccga gtgtgacggt cacactcgtc
    13381 aacacggcga ccggaggcgc gtcccgaact gttaccaatg aagtcggtct ttatacgttt
    13441 cctggtgtcg tgccgggacc gtaccggctc agcatcgcac atgagggttt cgagagattc
    13501 gaagtcgccc tcaccgtgca cgtgggcgag aacctcgctg tcgatccgct actcaaaccg
    13561 gggcagaccg caaccacggt cgaggttcgc gacgtcacgc cgatcgtaaa actcgacagc
    13621 ccgaccctcg gacacgtgct cgagcgccag aggatcgagc agttgccact caacgggcgc
    13681 accatcacca cactgctcaa cactgtgccc ggcatggaag gcaaccgcgc ctacggcctg
    13741 cgggaaggct cgcaggacat gatccttgac ggcgccaccc tgaccgaccg caactggggc
    13801 ggcaatccaa ggcgccagcc aggcctggat tccatccagg agttcaacgc cgaagacagc
    13861 ggcgcctcgg cgaagttcag ccgcccaacc ccgattctct actccacccg aagtggaacc
    13921 aacgatttcc acggctcgct gtttgagttc aaccgcaaca acggttacgg taaggcgcgg
    13981 cgccgcgaag aaggcaacaa gccctcgttc ctgaacagaa acgaattcgg cggcacggcc
    14041 ggcgggcccg tgtggctgcc aaagctttac gatggccgca accgcacctt ctggttcttc
    14101 tcccacgagg ctcaccgcaa catcgcgccc tactctgcgg aattcgaggt tccgaccgaa
    14161 gccatgcgca acggcgattt cagccaactc tacgatgatg cgggacggtt cattaagatc
    14221 tacaatccct ggacaaccga cccgaatacc tggtcgcgcg agcagttctc ctaccaggga
    14281 cgggccaatg tcatcgatcc ggccttgatg agcccgctcg ccaaaaagtt attcgacatc
    14341 acgccggcgc caacccatcc ggaggtgaat ccactcgtcg attcaaactg gattggcccc
    14401 gtgcccgaca cgacccgaca gtggacactc acaactcgcc ttgatcatcg tttctccgac
    14461 aatgaccagg tctatgttcg atatacgcaa ggctcgcacc accaactgtc gcaattctat
    14521 ggtcagccga tgctcaacaa cgtcgccggc ctgaggaacg tcaccgcccc gaaccggagc
    14581 gccgccgttt cctgggtccg aaccctttcg ccgacttggt tcaacgagct gctcgtttcc
    14641 ttcaatcgtg agaatgcttt ctccggcacc ggcgagcctg gcgtcaggta cgccgcagag
    14701 ctcggcttgc cgaacccatt taacagggaa ggctggccag ccatttacga aactggcctc
    14761 agcaactact acttcgagac ggagaatacc aagagaactc ctttcacgta tgcgctgatc
    14821 gacgataacg tcaccaaggt gatcggccgg cacgagttgc agctcggagg gcactaccgt
    14881 ttcgaccagc tcaactacat gtatgagcag cagtacacgc agggcgagca ccagttcgac
    14941 accctgtcaa cggcgctgta tgacccgagc tctccacgct ccgagcccta cgaggtgcag
    15001 aacaccggcc aatacctcgc caacatgttc atcggctcga cgaactactc ggcgaacttc
    15061 gaccgcggat acttttacgc acgctcgaaa gagtatgcgt tctacttgca ggacaggttc
    15121 cgagtaacgc ctcgactgac gctcagtctc ggcctccgct gggagtactg gccgccctac
    15181 cgcgagaagt acaacatgct ggtcgcctat gaccctgacc gcaaagcggc cgtgctcggc
    15241 tcggatctcg aatccatgta ccgcgtccag gccgcgcttc cctcgatcgt tgatcgctac
    15301 caacagctcg gtctccagtt cctgtcgtat caagaggccg gctggcctga aagactgatg
    15361 gatagcactc ggaagaattt cggtccccgg ctgggcttcg cctatcggct caccaatggg
    15421 gaccgcgccg ccgtccttcg tggcggctac cgtgtctcct acttcccgat cccgatgtcg
    15481 acctggggcc agcggcagcg catgaacgtg cccagccacg cccgcttcac gaactattcg
    15541 gctatcgact cctgggaatc gccggatggg atctccaact acagcctccg ctcggcgccc
    15601 accatcattg ccggcaagaa tagcatcgac gcgatcccta ccaatgacgc cagcttcatc
    15661 gcccgcggtc agggatcgct cagctatttt gccaaggaac agcccgacac gcgcgttcag
    15721 gactggaacc tgacactcga gaaagaggtc ctgccggata cggtcgcacg cgtcgcggca
    15781 gtcggaaacc acacctccaa tctcgagcag tactatcgtt acaacgaaaa tccgcccgcc
    15841 tacgtttggt acgcgcgaac cggattgccc agaccatcag gcgagttcgg caatgtcgct
    15901 atgcggcccc acgatcagca agtcctcggg accatcgaag agttccaaaa gagcggctgg
    15961 tccaactaca gcggcgtgca gttcgagctt gagcgccgct tcaacaaagg ctacggcttc
    16021 caggtcttct acaacattca caacgtcctg ggcgccggcg gtggtggtta tggagtctac
    16081 atcctcgatc ccacccagtt cctccccggc gcagtgccgg aagatatgca cgaccgcgcc
    16141 cggtttctca actaccggcg cgagcgcgac tacccaaagc atagtgttcg atggaactgg
    16201 gtcgccgaac tgcctttcgg taagggtaag ccattcgggg gcaatgccag cggcgctctc
    16261 gagaagttaa tcggcggatg gcagatctcc ggcatgggct atctgcgcag cacgtatttc
    16321 gagctgccca caaacctcta tccaaccacc ggcaatccgg tcgagatcta cggctacaag
    16381 tatccgatcc aggactgccg tagtggaacc tgccgctctg gctacctctg gtggaatggt
    16441 tacatcccgc cacacaagat caacagctac gacgccaacg gacaacccaa cggcgtgatg
    16501 ggtgtccccg agagctaccg gcccgctgga cagccgctca atccgtggcc ggccaatccg
    16561 gatccagccg atccgatgta cgactcgtac ggaacgaacc gcgtctgggt cacgctcaac
    16621 gacggttccc gccttcagac cgactacaac gacaaccttc acccctggcg caaccagtac
    16681 ttcccgagcg tgcgccagtg gagcatggat gcgcagcttt ccaagcgcat cccgatcacg
    16741 gagcgcgtcg cgctccagat caaggtcgat gccttcaacg tgttcaacgc tcccaacaac
    16801 ttcttccgcg gatgtgcgac ttgcagcgac ggcatcatct cgacgttgtc gtccgctcct
    16861 ggttatgcaa acttctaccc gagagaaatg caactcgggg cacgtttaac ctggtagtcc
    16921 tttcttgacc gacggaacgg ggcgagcgtg gaatgtgcgc cccgttctgt tccgctgtct
    16981 tgtagtcaac gcctggccgg caggatcggt ttcatccagg tcggcctaag aataagttcg
    17041 gcaggaaagc cgatgacgca cgagttcgac ggcaagaaat acgagagggc atccactcac
    17101 caaaaggaat ggggagcgaa gctcattgtg gaactctccc tgcagggaac cgagcgggtt
    17161 cttgatcttg gctgcggcga tggcactctc accgcacagc ttgcggatct tgttcccagt
    17221 ggcgaagtga tcggcatcga cgcctcccat ggcatgattg acgcagcgca acggaaaagc
    17281 agaaggaacc tgacattcct cgtgacggac atcaacgata tggatttcgt cgaacagttc
    17341 gatgtcgtgt tctcaaatgc cacactccac tgggtcaaag atcacggcat cctgctccgt
    17401 aacgtcaacc gcgccctgtg cgtcggcggg aggcttcgct tcaacttcgc tggagaggga
    17461 aactgccccc atttctataa ggttattcgg gaagcgatgt tgctggaggc gttcgcggat
    17521 tacttcagcg agttcgtatg tccgtggtac atgccatccg tcgatgagta ttccgccgtg
    17581 gtgaaagcaa gcggactggg cggtgcgcga gtctggggcg agaacgccga caggttcttt
    17641 cagaacgcag aacagatgac gaactggatc gaccaaccca gtcttgtgcc gttcctcgtt
    17701 cgcgtctccg acaggaaaaa gggcgcattt cgggaatacg tagtcagaag gatgatcgag
    17761 gagacacagc aggacgacgg acggtgcttt gagacgttcc gtcgaatcaa tgtgtacgcc
    17821 accacatgag catcgccgaa cccccgatcc agacgacgcc ttacggcgcc tctgatcggc
    17881 accgttaggg agccacaacg ctcggtcggt tccgccgatt cccctcagcc cagcgagacg
    17941 gtcttcaggt cgatcttgtc ggggtcgctc acgccgaggc cgagcaggcc ggcgagctcg
    18001 atgtgctccg gttgcatccg aacgaacacg ctgtcctcgt cggcgggagc gagcgcgatc
    18061 gtctgcatgc cggcctgcac gcgcttgtcg tcaataacgc ccagaccgat cgtatccacc
    18121 gcgacgggat cggtagcgaa gtataacgtc ttgtgctccc aaaggtactt gcccaccctg
    18181 ctgcctggcc ctccgtggta ggccgccttg acaccgtcga tgatattgag cacggtcttc
    18241 tgcttgatca ccggaatgtc gatgacggtc gggataaaca tcccgcaaga gttgttcgag
    18301 ctgctcacgt ggctgcgggc gacattattc acgagaccgt gcgacaggtt cttgagagcg
    18361 atcgtcactc ccgccgactg gtgatgcttc accacgccga gattgatcag tttattcgct
    18421 tccttcgtca gcaggcgggc cacgtaggac cgccggtgat ggggattcgt gggattcgcc
    18481 ttggcctcca ccagcgccat ctccatgtag tgatccgggt cgtatccttc catgtccaac
    18541 tggaacggag cgaccttctt ggtcgcatag ctccagcgaa cgccttcggg cagccacttg
    18601 tcgaagccgc cgcccaggaa ctgatcgtag tagcggtcgt aggcgatgat gtcgttccgc
    18661 ttgactccgg cttcctcgag ccccgcgacg atctcctgga acacctccgg cgcggaaatc
    18721 agatagggct ggccgacggg gtttaccttg atgatgacga catcgccggg ttcgaagaat
    18781 agacgccagg catccatcca ggcgggcgca ccggtcagtt ccatcatccc ccggcgcatc
    18841 atcgcgcgga ccggttcgcg ctggtaggtc ccggaaatca cactagccgg gtgtcgcacc
    18901 gcaacgacgc ggccggggta cggcccggga atccccagtt tctttttcgg cgatctctcc
    18961 gcgaggccgc gggcgaccgg gagacctgtg gccatcaagg ctcgattgat gaactgccga
    19021 cgttccatgt tgtcagaccc ctttccggac tcgctttgac ttccattttg gcgttccggc
    19081 tgggggaaag tccacttgct tcccggatcg cggcccaaaa tgtgcataat tgtgttattg
    19141 gcccaaaagg ccgggcgcaa tggatcaagt tggggggtac atgggaaatc cggggaattc
    19201 tttgcccgat gcacagtctg acatcgtggg gacgccgctt caggagccga acggcggcaa
    19261 gcgaatcgtg ctgagcgcgc cgctcacgga gacggtcgac cacgcagggt acttcattca
    19321 gatggctctc gcgtcgcttc ccgcgcggct tgagggcatc atcaactcga gatacccgcg
    19381 atggagggac ctcgagcgca acagcgatgg ttccgcacgg tggatgccgg cgggcgtccg
    19441 gctgcttgaa gcggcgctgc tccggcaata tccgcgggag gaactggtcg cctgttaccc
    19501 ggatgacctg gagcggttta tcgggcccaa gacaaagctg gtcgccgttt cgacccacaa
    19561 ccctctcggg gtcacgtttg cggcgggcgt ctacacctcc gtcttcggtt cctcacggga
    19621 acccatcaat tcgcactacg ccaagcaact ctttgccacg atcaaggcga atcccaaccg
    19681 ggcgaacttc aaagtgatcg ttggaggctc gggaggatgg cagatcattc agacggatac
    19741 ttgggacgag ttgggcgtcg attgcgtagt cgaggggcgc agcgaatccg aagatacgat
    19801 ggagctcatc cggcgggcga tccgcgggga gccgcttccc cgcaagctgg aggtcgccca
    19861 tccgaaagac cgcgacagca ttctggttcc ggaaaaccgg acgaccttcg gcgtcgttga
    19921 aatgacgaca ggatgcggac gccgctgcaa cttctgcgtg cccgacctca atccccaact
    19981 cgacgtagcg aaagagaaga tcatgcgggg cgtattgaca aacgtccgcc aagggaacac
    20041 gcaaatctca ctcgccacgg aggacatgtt tatctggggc caagtgcgca ctggagtgcc
    20101 gttttacttt cccaatcgcg aagcgctgct cgatctttac tccgagatcg ttggcactcc
    20161 cggcgtggag aaacatcttc tcagccacgc caccatagcg ccctccgttg tggatcccct
    20221 cctgatcgag aagctgtcgg acgttctcct cgacaagagc ccgatccacc tgccgacgca
    20281 cagctctcac ccgcgaaaga aaatcctgag tccgctgatc gggctggaga ccggatcggt
    20341 ccgactcgcc cgggcgatca tgcccggcaa ggccgttccc ttttcgatcg acgactggcc
    20401 gagcgtcgtg gtgcaggggc tgaccgtcct gaaccgcaac aactggtttc cggtgatgac
    20461 gttgatgatc ggaaacccgg gcgagacgga cgaagacgtt aaggcaacgc tcgatctggt
    20521 ctatgagatc gagcggagag ggctgtttgc attcctcgtt ccctccatat tcacaccgct
    20581 gcacgacacg cgcatggaga agaagaaggg ggtcacggaa acccgaaacc tcagtccctt
    20641 gcagtggcaa ctgatgatga aatgctggaa gatgaacctc cggcccgggc aatacagttg
    20701 gtggggaccg acggcctggc gattgggtgc gattggtctc tggctgtaca agcttcgccg
    20761 cgtaaacggg ccgaacttca cctggccctt gctaatgttt gccagcgcag ttcccgagcg
    20821 ggtaatggag cgcatgggca agatctacat cggcaaacct atagcgatca agagccgaaa
    20881 ggagctgctc gctacggtca aacgggagca ctgggtacac cttcgccgcg acaccgggga
    20941 cctgccagac gactacgagg catcggtcac cgtaggtcgg gcttcctccg ctttccgggt
    21001 gacctccgcg agttgaacgc ctacttcagc tccgccaatt tcacatcgag gaaggcgaga
    21061 gaatcctgaa gaatcttccg gtatcgggga ttccggcatt cttcgaggtc gtgcagaacc
    21121 cgcgtgcgtg atagcagcag gttcgccttc tcacgtcggt gctcaaattg ttccggagtc
    21181 agttttcgtt cgccgccatg ctggcggccg tctaggtgtg aatcgatttg tgattccaca
    21241 gatttgcttt cccaacctcg ggccacactt tcattgtaat gccattacgg acaggctgcg
    21301 ttccgatgac agcaggcgcg ctgtgctata aacgccggag gaggatctta atcttgtcat
    21361 ccagtcgtcg ggacttcgta aagacagccg ccctcgccgc cgcaccggcg gtccgccccg
    21421 cgtggggcca aagcagcccg aataaccgca tcaatattgg cgtcgtcgga tttcacggcc
    21481 gtggccggga tcactatcgc gagtttgccc ggatgcccaa tgtgaaggtc gcttaccttt
    21541 gcgacatcga cgagcggctt tatccaggcg ccgtcgcgga gatcgagaag atggcgggga
    21601 acaaaccgga aaccttcgtc gatctgcgga agatgctcga gaagaaggac ctcgacgcga
    21661 tttcgatcgc cactccggac cactggcacg cccttcagat gatctgggct tgccaggcgg
    21721 gcaaagacgt gtacgtcgag aagcccgtct gccacaatct gcgcgaaggc cgcaagatgg
    21781 tggaggccgc gcgcaaatac aaccgcatcg tccaatgcgg gctgaaccgg cgcagcgaag
    21841 ctcgtacccg caccggtgtg cgcttcgtac aggaagcgaa gttcgggtcg gcataccgcg
    21901 ccaaggcggt tgtgtatcgg ggccgcacga gcatcggccg ggtgcaggag tcatcgattc
    21961 ccaacggcgt gcactgggac ctgtttctcg ggccggcgcc gtaccgcgcc ttcacgctga
    22021 accgttttca ctacggttgg cactattact gggatacggc cacgaccgag gtcggaaaca
    22081 acggcgtcca cgccatcgac gtggtgcgat gggcgacgaa caggaacgtg catccggtca
    22141 agatccattg cgcgggaggg cttttcgcgg acaactccga gcaggagacg ccgaacgtgc
    22201 agaatgcgtc ctacgagtac gcggacggca cgctgctgga actcgaggtc acaacccttc
    22261 cgagcccgac attcggtggc gttcacatgg gcgaattttt ctacacgccg cagggttatg
    22321 tgacgtcggc gaacaagtgg tcgacggtgg ttggcgagtt tacggtgcgg aaccgcccga
    22381 atcctcccag tggcgtcgac gaacgcatgg ggaacatcag cttcccgaac atcgcctaca
    22441 aaccggggcc ggtgatcccg gagtccacgg agacggaagt gagccacttc cagaacttca
    22501 tcgactgcgt gcggtcgcgg aaacgggagg atctgcactg tgagatgatc gagggacaca
    22561 tgtccacatc gctgtgtcat ctggcgaaca tcgcattccg aacggggcgg aagctgacct
    22621 tcgatccggc ggccgagacc ttcccgggcg acgccgaggc caacgccctg ctgacccgca
    22681 agtacc
//
LOCUS       DATIFQ010000291        15896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2776612, whole genome shotgun sequence.
ACCESSION   DATIFQ010000291 DATIFQ010000000
VERSION     DATIFQ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15896
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2776612"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VLE22_13640"
     CDS             complement(<1..210)
                     /locus_tag="VLE22_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15494.1"
                     /translation="MKLWAVLFAVAVVAAGQTAPQTEERGVRVVRHVTAESGTAPGEP
                     VKDVVRFVSSEFAWSDKLVKNAPYSA"
     gene            complement(262..843)
                     /locus_tag="VLE22_13645"
     CDS             complement(262..843)
                     /locus_tag="VLE22_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15495.1"
                     /translation="MTDRESEKVGSLLRELASGMAAEQAPVELEGRLLAAFKRHRAAR
                     FRRRAATAGLIAASIAILVFSARLVGDRDAAPAPRAASAPVSEPVNAPAVAAPETAPK
                     RRPKPRMARTARRPRAAARQRDLEVATGFIPVAGEDMLGSLDRGYLVRVELPRTALRS
                     FGLPMNEDRAAERVMADVLMGEDGLARAIRFIQ"
     gene            complement(840..1403)
                     /locus_tag="VLE22_13650"
     CDS             complement(840..1403)
                     /locus_tag="VLE22_13650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSB15496.1"
                     /translation="MNDSADEELLGQMVSGSEAAFVVLYRRCQGPTYRFALQMTGSRE
                     IAEEVTQEVFVTLIHSGGRYDPSRGPFLSWLLGIARNHVLRCLERERRYVGVEEGDFE
                     RDDPPEAEDPLAELSREEACRNLREAIPLLPIRYREAIVLCDLQEMSYEDAARTLDCA
                     VGTVRSRLHRGRALLAAKLRGRERCLS"
     gene            1795..3858
                     /locus_tag="VLE22_13655"
     CDS             1795..3858
                     /locus_tag="VLE22_13655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HSB15497.1"
                     /translation="MRIPFLKNWAFRFALVLTFVVGSIVLVSAPGPGFGPHDKAFYAD
                     ANLVNFVRPGLVFNIVSAEIAADGTAKVRFKLTDPKGLPLDRLGVTTPGAVSTSFILA
                     RIPAGKTQYESYTTRSQTSPITNQTAIQAGADSGGTYEQVGDGEYIYTFKTKLPSGYD
                     KSATHTVGMYGNRNLTEFDLGTNSTETEFHFVPDGSKPPAPRDVIKTATCNKCHQHLT
                     AHGEGGRQLMEGCILCHQPQTKDPDTGETVDMAVMIHKIHQGEGLPSVQAGKPYIIIG
                     NRQSVHDFSEVVFPADTRNCTFCHEQNTGAVQADAYFKPNMAACGSCHDNVNFATGEN
                     HANLPQVSNNQCANCHTPDGEVEFDISITRGHTIPRFSRDLPGFVFELVKVDEAGPGK
                     NPVVTFKLTDKAGKPIIASEVTRLALVLAGPTSDYGWYVSEDAKSATAGQDGTHTYTF
                     KAALPADAKGTYAVGIEGYRNMVLLANTPKQVTVRDAGVNKQIYFSVDGSPVQPRRQV
                     VALNIPDTPYGGCNKCHASLSLHGDNRNQIEQCVMCHNPNESDKARRPADKLPAQSVD
                     FRMMVHRIHTGEELPYDFTIYGFGGNPINFNKVLYPGDRRRCNSCHVNDSQQLPLNDN
                     LLNVTDPRGVLNPVGPTTAACTSCHTAVYAASHALANTTPLGESCAACHKPGADFALD
                     RVHAE"
     gene            3905..4855
                     /locus_tag="VLE22_13660"
     CDS             3905..4855
                     /locus_tag="VLE22_13660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HSB15498.1"
                     /translation="MSGGPAWAFLAVLLCAPAMPAVQAAGEQKQEQKPAKQAATYVGS
                     ETCQACHEDIYKAFQRNPHHAVEKDARRKWEGKACESCHGPGSAHAESTEKKDIVNPA
                     GLAASEADRGCLGCHLNQPTHVGRVFGGHGKNQVSCVSCHSIHGTAPESLVARKASTV
                     NKKCAECHTPVWAEFQRPHKHRLPEGAMSCVDCHNPHGSFLPRSMQTVSANEPGCFKC
                     HGDKRGPFTYEHAPVRFEGCTACHQPHGSANPRMLNRHEVRLTCLECHANLPLPTLAG
                     SNGLGGVPPSFHDLRSPRYRNCTICHVKVHGSHVNRALLR"
     gene            4852..6837
                     /locus_tag="VLE22_13665"
     CDS             4852..6837
                     /locus_tag="VLE22_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15499.1"
                     /translation="MRLILAIFLAVVTLAAQEPSAQPAQEAPSPAPATEQWLTGNIEF
                     GNRWVSDVRGNAEAYRSVVNLGEGPKLFGLDLTLTDPSRRLFDSAVIRANSWGGDPYN
                     TASVDVRRERSYNLSFDYRNIAYFNSLPSFANPAVDRGIFLSQRSFDIQRRMIDTELE
                     LMPGSRIIPYVAFSRDWGAGSGVTSFVTDGNEFAVANDLRDRTNNFRGGVRVELNRFH
                     ATFEQGGTNFKDHQRLFHSPGPHYGNRTVPLLGRQTLLSTLEQAYRVEGDSVYTKGLV
                     TTNPASWVNLYGQFLYSRPDSDVSYSQDNSGKFVSFDPPLSSLRLFDSQMVLLTSESK
                     QPHTSGSFGAELRLHRRIRVTEAVMTDRFHTTGSALLQETLAAGTVSATSSGVPPGLE
                     KLIFNYSRQEVNVLADVLPRLTLRGGHRYVWGDAQVRAPQLSQTGTVENGELKMHVGL
                     AGFVFRAAQKLTVSADFEGGSADRNYFRTSLQDYKKLSARANYQVLNSLNMGARFSLL
                     DNQNPAQNIRNDFLSRDNSITVLWTPAGGKRVSLLGDYSRSTLRSDLLAVVPSTLQSE
                     LSTYAERAHTGTALLEINLPDVQKASPKLSLGGSFFKSSGSRPTEYYQPIGRFSVPFH
                     RKVRGFFEWRWYRLFESFYQYEGFRTHQFVGGLQLAL"
     gene            6846..7961
                     /locus_tag="VLE22_13670"
     CDS             6846..7961
                     /locus_tag="VLE22_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSB15500.1"
                     /translation="MHRSLIAATCSVFALQLTFGAIEIPGDARQGEQVFKSQRCVECH
                     SVNGVGGKSAPDLGKRTARNYTPAMMASLMWNHAPKMWSAMSDKAVTRPQLTSEQAAD
                     LFAFFYASRYFERPGDAGRGKQAFQTKGCAGCHSLSGVAGSGPKAVASWKSLDDPILL
                     AQEMWNHAALMKEALGKKRMAWPRLTSQELTDMLVYLQNLPETRGRQTEFAPASAETG
                     KMLFDLKGCRNCHTGKQSLENRFANRTIVDFAVAMWNHAPKMAQPAPELRAEEMRRIA
                     GYLWSIQLFDSPGNAARGKSVYQTKNCATCHEDASSGAPRIVVRDGVSSPQRMVHELW
                     RHGPAMLEVMKKKNIAWPQFAGNEMADLIAYLNSSGS"
     gene            7975..8655
                     /locus_tag="VLE22_13675"
     CDS             7975..8655
                     /locus_tag="VLE22_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSB15501.1"
                     /translation="MRVQTTEVLSKISLFAALTPPELDALAARTLQRRYAAGEVLFRE
                     GDPCYGFFVLGRGTVKIFKTSSSGREIMLAVETAPSSVAEVPLFDGGPYPATVSAIDE
                     VDAYVISKQDFRSVCRQNPELALKILAVVGKRLRQLVGVVESVSFGSVRQRLARNLLD
                     FRHEYGQDRFILPVTHQELASRLGTVREVISRNLSRFQAEGLIKIEKREVLIVDSEGL
                     EREAETEI"
     gene            8930..9598
                     /locus_tag="VLE22_13680"
     CDS             8930..9598
                     /locus_tag="VLE22_13680"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HSB15502.1"
                     /translation="MTVLLVLFTFLVFIAVDYILTRKKAAQPHAAPDVVASPVLDPAY
                     VEGFLVPEKLQYHPGHSWAMRERKHLLRVGIDEFGAALMGRIEKIEVPKPGVWIRQGQ
                     KAFSFFRDGEKTEVLSPTEGEVIEVNNDLLNDPSLLRKDPYGAGWLMMVHVPDEETVN
                     RNLIPKNMVRGWIRDAVERLYARQPQFAGAVAADGGRPADDLFAALPGESWKELTGEF
                     FLSK"
     gene            9701..10471
                     /locus_tag="VLE22_13685"
     CDS             9701..10471
                     /locus_tag="VLE22_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSB15503.1"
                     /translation="MKQRAVLYDSTLCVGCRACEEACARKWNLPYNDAVASKEQLSEN
                     KLTTIATYGERYARRMCMHCAEPTCVSVCPVGAFQKTPLGPVIYLEDRCMGCRYCMLA
                     CPYSVPVYEWSKTLPKVRKCDMCYDRVRAGKPTSCSEACPTGATITGERNALIKEAQR
                     RMTENPSQYNGRIYGLEEVGGTSVLMLAGVPMEQLGVRANLPNAPLSLATHAALSFVP
                     DIVGVGAVLLSGVYWITHRREEVAAAEGGPQKGREKTK"
     gene            10468..11646
                     /gene="hybB"
                     /locus_tag="VLE22_13690"
     CDS             10468..11646
                     /gene="hybB"
                     /locus_tag="VLE22_13690"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HSB15504.1"
                     /translation="MTRLLRLPRLTFWQLVFALLMLVGAYATYVRFFHGLGAATNLND
                     RFPWGIWIGFDVLCGVGLAAGGFTLAATVHIFNIEKYKPIVRPAILTAFLGYTLVILA
                     LLFDLGRPYRVWHPLVMWNPHSVMFEVGWCVTLYTTVLAFEFAPMVLERIGWHAGLKW
                     VRSAMIPLVIAGVILSTLHQSSLGSLYLIVPHKLHPLWYTPLLPILFFVSAICVGLAM
                     TIFESWHSAKACGRYLELRLLAGLSRVLAVLLLAYLALRFLDLARRGALRLILEQRME
                     SYLFGLEIALMLLPMLLLFRAHVRRRAGALYACSVMVIFGFITHRLNVSVTGMEAGSG
                     VQYLPKWTEIAITLAIVAAGFAIFRFATKYLPIFGEEEQHSEVVAVAPEALPSPVRGD
                     "
     gene            11699..13300
                     /locus_tag="VLE22_13695"
     CDS             11699..13300
                     /locus_tag="VLE22_13695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB15505.1"
                     /translation="MKLAVCVVASFTAFVALFSYLGFREHRRHSEDLVVTSADRLTDV
                     IQRSTSYQMLHNDRAALYQMIHAIGSEPGIRRVRIFNEDGRISFSTSETEVGTVLDKQ
                     AEACYACHAQAAPLTKLDRPDRARIFPEENAKRVLGIIRPIENQPACSNAACHAHPPQ
                     RRILGVTDVQISLESVDAQLAEYRRHLLAITGLSVLLGSLVSVGFILLFVHRPVGELI
                     AGTQTVAGGNLGHRLPVRSGDELGELAESFNKMTEDLERAHLEITTWARTLEERVEKK
                     SAELQRVHESLLTSEKMASLGKLAATVAHEVNNPLFGILTYARLSLRALEKSDLSPES
                     KAQVLDQLRTIERESRRCGEIMKNLLNFARQVPPHREAHDLNVLVDRAITLVRHQLAL
                     QEIELEKRLQGDLPACFCDADQIQQVVLALLINASDVMPGGGTIRVTTESVKSGENAC
                     IRVADTGGGIDSETLPKIFEPFFTTKEDQHRTGLGLAIAKGIVERHGGFITVISKPGE
                     GTEFTILLPVGEPIAAGPDGKGNRN"
     gene            13297..14652
                     /locus_tag="VLE22_13700"
     CDS             13297..14652
                     /locus_tag="VLE22_13700"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB15506.1"
                     /translation="MSVPETKSSSLGRILVVDDELVVRDSLDKWFAGEGYETGVAASA
                     REALEMFQKNEWDLVLLDIKMPGMDGMELQARLREIDPELVIIIMTGYASVETAVQAL
                     KRGAYDYVTKPIDPDELVHLVSNAIGHRRDRREIVRLRENLQEIYPETHLIGNSPPMK
                     HVLELVEMVAPTDTTVLISGESGTGKELVARAIHAAGSRRYMPMVVIHCGALTETLLE
                     SELFGHERGAFTGAQYRKKGKFEVADGGTVFLDEISDISLKTQTDLLRVLQEKEIVRV
                     GGNQSIKVDFRCIAATNKSLEELVKAGTFRPDLFYRLKVFSIELPPLRDRREDIPLLA
                     DHFIKKYATAMNRPEPRLSPEALDLLLSHNWPGNVRELENAIERALVIGREGLIQPSD
                     FPFQVHSQSPQEGRTLEDVERVHIERVLRETAGNLSRTARILDIDRTTLYNKLKRYGL
                     K"
     gene            14649..15206
                     /locus_tag="VLE22_13705"
     CDS             14649..15206
                     /locus_tag="VLE22_13705"
                     /inference="COORDINATES: protein motif:HMM:NF019610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin"
                     /protein_id="HSB15507.1"
                     /translation="MISLPRRIAAIHLQPVGDGVTLEMLDRICAALARLFEVSCHVRG
                     ERLDAGLALDPVRLQYFSTAILQQLVPLAAGDEEIRLLGITEYDLFVPVLTFVFGEAQ
                     LDGRCGLVSLHRLREEFYGMPASEELVEERAVKEVIHELGHTFGLRHCPDWSCAMAST
                     HAVERLDLKEARLCADCTRRLGAWS"
     gene            complement(15255..15857)
                     /locus_tag="VLE22_13710"
     CDS             complement(15255..15857)
                     /locus_tag="VLE22_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15508.1"
                     /translation="MPIVLVALLGFFVVTATGQDSAAPSYLSAIPSSHPDSNLQYYIK
                     QTFGPAAYVGSAVTAGWNQWRNSPPEWEQGGDGFERRLASAYARRVARNSIDLGVASL
                     RGEVLAYDHCECSGFWPRTEHAVKRTFVRRTENGGQTFAVGRFAGAYGSGFLANTWYP
                     DSRNDFRHGLARGSTTLGIELGKNVFREFWPDIKHKLFRR"
BASE COUNT         3094 a         4907 c         4834 g         3061 t
ORIGIN      
        1 cgccgaatac ggagcgtttt tcacgagctt atcgctccac gcaaactcgc tcgagacaaa
       61 acgcactacg tccttcaccg gctcgccagg cgccgtccca ctttctgccg tgacgtgccg
      121 aacgacccgg acacctcttt cctccgtctg aggcgccgtc tgtccggccg ccaccacggc
      181 cacggcgaac agtaccgccc aaagcttcat ttgttctctc atcggtgact ctccctcggt
      241 cttctcgatt cgaacttcta actactgaat aaagcggatc gctcgtgcca gtccgtcttc
      301 tcccatcagc acgtccgcca tgactctctc ggcggctcgg tcctcgttca tcggaaggcc
      361 aaaactccgc agcgccgttc ggggcaactc gacccgcacg agatacccgc ggtcgagcga
      421 cccaagcatg tcctcgcccg cgaccggaat gaaccccgtg gcaacctcga gatcgcgctg
      481 tctcgctgct gctcggggcc ggcgtgccgt ccttgccatt cgcggtttcg gccgccgctt
      541 cggagccgtc tccggcgccg ccactgccgg cgcgttgacc ggttcggaga ccggcgcgga
      601 cgccgcccgc ggcgcgggag ccgcgtcacg gtcgcccacg agccgcgcgg agaataccag
      661 aatcgcgatg gacgccgcga tcagccccgc cgtggccgcc ctccgccgga accgggcggc
      721 ccgatggcgc ttgaatgcag ccaaaagcct gccctcgagt tccaccggcg cctgctccgc
      781 cgccatcccg gaggccagct ctcggagcag tgatccaact ttctccgatt cgcggtccgt
      841 catgatagac acctctcccg gccacgcagc ttcgccgcta acagcgcccg tccgcggtgc
      901 agccgggacc gcaccgtgcc caccgcacaa tccagcgtcc tcgcggcgtc ctcatagctc
      961 atttcctgca gatcgcacag cacaatcgcc tcccggtaac gaatgggcag cagtggaatc
     1021 gcttctctga ggttccggca cgcctcctcc cggctcagtt ccgccagcgg gtcctctgct
     1081 tcgggcggat cgtcccgttc gaagtcgccc tcctccacac ccacgtatct cctctcccgc
     1141 tccaggcagc gaagaacgtg attgcgtgca atcccgagca gccaggaaag gaaaggacct
     1201 cgggatgggt cataccggcc tccggaatgt atcagcgtca caaaaacctc ttgcgtcact
     1261 tcctcggcga tctcccggct cccggtcatt tgcagcgcaa atcggtacgt cggtccttga
     1321 caccgccggt acagaaccac gaaggccgcc tcactacccg agaccatctg cccgagaagc
     1381 tcttcgtccg cggaatcgtt catcgctatc tcggtttgac gggctggcat gggccgctgt
     1441 ttgggattca gggatctagg gcaagaagtt ccgccattat cgctttttcc agcaggccaa
     1501 gctaatgttg attttttcaa cgtcacgttt gcgtactttc acccagggtg tggtaatttg
     1561 ccccacttaa cctaacccag ccatcggccg gtcggatggg tgccgctgta accgattagg
     1621 aatgttgcca gttgtcgggc ggtcgcgcgc tacggagttt gggctgtccc ctgctgaata
     1681 gaaccggctt tgtttgcacg gtccctcgct cgacgtatgc gagatgcgag agttgtggtt
     1741 gagttgtaag gaattttcga agtagtctgt agcgacattt ggaggaaggt gaccatgcgc
     1801 attcctttcc tgaagaactg ggcgttccgt tttgcccttg ttctgacttt cgtcgtcggc
     1861 tcgatcgtcc tggtcagtgc acctggacct ggcttcggcc cccacgacaa ggctttttat
     1921 gcggacgcca acctggtgaa cttcgttcgg ccggggttgg tgttcaacat cgtctcagcg
     1981 gaaatcgccg ccgacggcac cgccaaagtc cggttcaaac tgacggatcc caagggtctg
     2041 cccctggacc ggctgggtgt gacaactccg ggagcggtaa gcacgagctt catcctggct
     2101 cgcatcccgg ctggcaagac gcagtatgag tcgtacacga cacggagcca gaccagcccg
     2161 atcacgaatc agacggccat ccaggccgga gcggactccg gaggcacata cgaacaggtc
     2221 ggcgatggtg agtacatcta cacgtttaag acgaaactcc cgtcgggtta cgacaagtcc
     2281 gccactcaca ccgtcggcat gtacggcaac cggaacctga ccgagttcga cctcggcacg
     2341 aacagcaccg agacggagtt ccacttcgtt cccgacggct ccaaaccgcc cgcccctcgc
     2401 gacgtgatca agaccgccac ctgcaacaag tgtcaccagc acttgacggc gcacggcgag
     2461 ggtggccgtc agttgatgga aggctgcatt ctctgccatc agccgcaaac gaaggacccc
     2521 gacaccggtg agaccgtgga catggctgtg atgatccaca agatccacca gggcgaagga
     2581 ctgcccagcg tgcaggctgg caagccgtac atcatcatcg ggaaccggca gtccgttcat
     2641 gacttttccg aagtcgtgtt ccccgcggat accaggaact gcaccttctg ccacgagcag
     2701 aataccgggg ctgtacaggc cgacgcatat tttaaaccga atatggcggc ttgcggctcc
     2761 tgtcacgata acgtaaattt cgcgactgga gagaaccacg caaatctgcc gcaggtttcc
     2821 aacaaccagt gcgcgaactg tcacacaccc gacggcgaag tggagttcga tatctccatc
     2881 acgcgcggac acaccatccc gcgcttctct cgcgaccttc ccggctttgt gtttgagcta
     2941 gtgaaagttg acgaggccgg gccgggcaag aatccggtcg tcacgttcaa gctgacggac
     3001 aaggccggca agccgattat tgcgtctgaa gtgacacggc tcgcgctcgt gctggccggg
     3061 ccgaccagtg attacggctg gtacgtctcc gaggacgcca aatcggccac cgccggacag
     3121 gacgggacgc acacgtatac gttcaaggcg gcgcttccgg cggacgcgaa aggaacttac
     3181 gctgtcggaa tcgaaggcta ccgcaacatg gtcctgctgg ccaacacgcc gaaacaggtg
     3241 acagtccgtg acgccggcgt caataagcag atctacttct cggtggacgg ctcgcccgtc
     3301 cagccgcgcc ggcaggtcgt ggcactgaat ataccggaca ccccgtacgg cggctgcaac
     3361 aagtgccacg ccagcctgtc gctgcatggt gacaaccgca accagatcga acagtgtgtc
     3421 atgtgccata acccgaacga gagcgacaag gctcggcgtc cggcggacaa gctgcccgcg
     3481 cagtcggtgg acttccggat gatggtccac aggatccaca ctggcgaaga actgccgtac
     3541 gacttcacga tctacggctt cggcggcaat ccgatcaact tcaacaaagt gctgtacccg
     3601 ggtgaccgcc gccgctgcaa ctcgtgccat gtaaacgatt cgcagcagtt gccgctcaac
     3661 gacaacctgc tcaacgtgac cgatccgcgc ggagtgctca acccggtcgg gccaacgacc
     3721 gcagcctgta ccagttgcca caccgctgtg tacgctgctt ctcatgcgct ggccaatacg
     3781 accccgctgg gcgagagctg cgcggcttgc cacaagcccg gagctgactt cgcgctcgat
     3841 cgcgtacatg cggagtaaac gcgcagtaca ctcacctccc tcctttgtcc cgcggggctc
     3901 ccggatgtcc gggggccccg cgtgggcgtt tcttgctgtg ttgctctgcg cgccggcgat
     3961 gcctgctgtt caggccgccg gagagcagaa acaggagcag aaaccggcga aacaggccgc
     4021 tacctacgtc ggctcggaaa cctgccaggc gtgtcacgag gacatctaca aggcgtttca
     4081 aaggaatcct catcacgccg tcgaaaagga cgcccggcgg aagtgggaag gcaaggcctg
     4141 cgaatcctgc cacggtcccg gcagcgccca cgccgaatca accgagaaga aggacatcgt
     4201 caaccccgcc ggacttgcag cctctgaagc cgaccgtggt tgcctcggct gccacctcaa
     4261 ccaacccacg catgtcggcc gtgtgttcgg cggccatggg aagaaccagg taagctgcgt
     4321 ctcctgccac tcgattcacg ggacggcgcc ggagagcctg gtggcgcgga aagcttccac
     4381 cgtgaacaag aaatgcgccg aatgtcacac gccggtttgg gccgagttcc agcggcctca
     4441 caagcatcgc ctgccggaag gcgcgatgtc gtgtgtcgac tgtcacaacc cgcacggcag
     4501 tttcctgccg cggtcgatgc aaacggtgtc agccaatgaa cctggctgct tcaagtgcca
     4561 cggcgacaag cgcgggccgt tcacgtacga gcacgctccc gttcgcttcg agggctgcac
     4621 ggcgtgtcat cagccgcacg gctcggccaa ccccaggatg ctgaaccgcc acgaagtgcg
     4681 gcttacatgt ctcgagtgcc atgcgaatct gccattgccg acgctggctg gctccaacgg
     4741 cttgggcggc gtgccgccgt cgttccacga cctgcggagt ccgcgctacc gcaattgcac
     4801 catctgccac gtgaaggtcc acggatctca cgtcaatcgg gccttactgc gatgagactg
     4861 atccttgcca tctttctagc cgtcgttact cttgccgcgc aggaaccgtc cgcacagccg
     4921 gcccaggagg cgccgtctcc ggcgcccgcc accgaacagt ggctcaccgg gaatatcgag
     4981 ttcggcaacc gctgggtcag cgacgtacgc ggaaatgccg aggcgtaccg cagcgtcgtc
     5041 aacctcggcg agggtcccaa gctgtttgga ctcgacctca cgctcacgga tccatcccgc
     5101 cgtctgtttg acagcgcggt catccgtgcg aacagttggg gcggcgaccc gtacaacacg
     5161 gcgagcgtcg atgtgcggcg cgagcggtcg tacaacctct cgtttgacta ccggaacatc
     5221 gcctacttca attcgctgcc gtcctttgcg aaccctgccg tcgaccgcgg catcttcctg
     5281 agccagcgct ccttcgacat ccagcgacgc atgatcgaca cggaactgga gctcatgccg
     5341 ggaagccgca tcattcctta cgtcgctttc tcgcgggatt ggggcgccgg ctccggtgtt
     5401 acgagtttcg tcaccgacgg gaacgagttc gccgtggcta atgaccttcg cgaccgcacg
     5461 aacaacttcc gcggcggagt ccgcgtggaa ttgaatcggt tccatgcaac gttcgagcag
     5521 ggcggaacga acttcaaaga ccatcaacgg ctgttccact cgcccggacc gcattacggg
     5581 aaccgaacgg tgccgcttct cgggcgccag acgctgctgt ccacgctcga gcaggcctat
     5641 cgcgtcgagg gcgacagcgt ctacacgaag ggtcttgtta caacgaaccc tgcgtcgtgg
     5701 gtcaacctct acggccagtt cctctacagc cggcccgaca gtgatgtaag ttattctcag
     5761 gacaactccg gcaagtttgt ttcgttcgac ccgccactgt cctcgttgcg attgttcgac
     5821 tctcagatgg tgctcctcac ctcggagtcg aaacaacccc atacgtcggg cagcttcggc
     5881 gccgagctcc gattgcaccg gcgcatacgg gtcaccgaag ccgtgatgac cgatcggttc
     5941 catacaaccg gttcggcgct tttgcaggaa acgctcgccg ccggaacggt ttccgcgacc
     6001 agctcgggcg tgccgccggg cctggaaaag ctgatcttca attacagccg gcaggaagtg
     6061 aacgtcctgg ccgacgtact gccccgcctg acgcttcgcg gcggtcatcg ctacgtgtgg
     6121 ggtgacgctc aggtgcgcgc tccgcaattg agccagaccg gtaccgtcga gaacggtgag
     6181 ctgaagatgc acgtgggcct agctgggttc gtcttccgtg ccgcccaaaa actgaccgtg
     6241 agcgcggact tcgagggcgg ctccgccgac cggaactact tccggaccag ccttcaggac
     6301 tacaaaaagc tgagcgcccg ggctaactac caggtgctga actcgctgaa tatgggtgcg
     6361 cgtttctctc tgctggacaa ccagaacccc gcgcagaaca tccggaacga tttcctcagc
     6421 cgcgacaact cgattaccgt gttgtggacc cccgccggcg ggaagcgtgt cagtctcctt
     6481 ggagactact cgcggtccac gctgcggtcg gatcttctgg ctgtggtacc gagcaccctc
     6541 cagagcgagc tatccacgta cgctgagagg gcgcacacgg ggacggctct gctcgagatc
     6601 aacctgcccg acgttcaaaa ggcttcgcca aagctgagcc tcggcgggtc gttcttcaaa
     6661 tcctcgggca gccgtccgac cgagtactac cagcccatcg gccgtttttc ggtcccgttc
     6721 catcggaagg ttcgcggctt ctttgagtgg cgctggtacc gcctgttcga gagcttctat
     6781 cagtacgaag gatttcgcac gcatcagttc gttggaggtt tgcagctcgc gttatagtag
     6841 cgggtatgca ccgatctctg attgccgcta cctgtagtgt ctttgcgctt caactgacct
     6901 tcggcgccat cgagatcccc ggggatgccc gccaagggga acaggttttc aagagccagc
     6961 gctgcgtgga atgtcacagc gtgaacggcg tgggcgggaa gtccgctccg gacttgggga
     7021 aacgcacggc gcgcaactac acgcccgcca tgatggcgag cctgatgtgg aaccacgctc
     7081 ccaagatgtg gagcgccatg tccgacaagg ctgtgacccg cccgcagttg accagcgagc
     7141 aggcggccga cctgttcgcc ttcttctacg cctcccgcta tttcgagcgg cccggcgacg
     7201 ccggccgagg aaagcaggca tttcaaacca agggctgtgc cgggtgccac tctctgagtg
     7261 gagtggccgg ctccggaccg aaagccgtgg cctcctggaa gtcgctggac gatccgatcc
     7321 ttctggcgca ggaaatgtgg aaccacgctg cgctgatgaa ggaggcgctc ggaaagaaac
     7381 gaatggcttg gccccggctg acgagccagg agttgaccga catgctcgtg tacttgcaga
     7441 acctgcccga gaccaggggg cgccaaacag aattcgcccc ggcctcggcc gaaaccggca
     7501 agatgctgtt cgatcttaag ggttgcagga actgccacac ggggaaacag tcgcttgaaa
     7561 atcggttcgc caaccgcacg atcgtcgatt tcgccgtcgc gatgtggaat cacgccccga
     7621 agatggctca gcccgcaccg gaactgcgcg ccgaagaaat gcgccggatc gcgggttatc
     7681 tctggtcgat ccaactcttc gacagtcccg gaaatgccgc gagaggcaag tccgtctacc
     7741 agacaaagaa ttgcgccacc tgccacgaag atgcttcgag cggcgccccg aggattgttg
     7801 tccgggacgg cgtcagttcc ccgcagagga tggtccacga gctctggaga cacggtccgg
     7861 caatgctcga ggtgatgaaa aagaagaata tagcctggcc gcagtttgcg ggcaacgaga
     7921 tggccgactt gatcgcgtac ctgaattcgt ccgggagttg acggacccgg tccaatgcga
     7981 gtacaaacca ctgaagtcct ttccaaaatc agcttattcg ccgccctgac gccgccggag
     8041 ttggatgctc tggcggcgcg aaccctccag cgtcgctacg cggcgggcga agtccttttc
     8101 cgggaaggag atccatgtta tggcttcttc gtcctcggcc gtggaaccgt aaaaatcttc
     8161 aagacttcgt cgagcggccg ggagatcatg ctggcggttg aaaccgctcc ttccagcgtt
     8221 gcggaagtcc ccttgtttga tggcggcccg taccccgcca cagtcagcgc catcgatgaa
     8281 gtcgatgcgt acgtcatcag caaacaggat ttccgctccg tctgccggca aaaccctgag
     8341 ctcgccctca agatactcgc cgtcgtaggt aagcggctcc ggcagctcgt cggcgtcgtc
     8401 gaaagtgtca gttttggctc cgttcggcag cgtttagcgc gcaatctgct cgattttcgg
     8461 cacgaatacg gtcaagatcg gttcatcctg ccggtcacac accaggagct ggcttcccgc
     8521 ctgggtaccg tccgcgaagt gatctcccga aatcttagcc gattccaggc tgagggcctc
     8581 atcaagattg agaagagaga ggtgctgatc gtcgactccg aaggcctgga acgcgaggct
     8641 gagacggaga tctagttcgt tttttcgtgt atttcctcga cacggtatgt cgatatattc
     8701 aacagcgcgc taaccactct gttcgttgat atatagctct taagctctgg ttatcgccgc
     8761 tgggtagtgt tgtattagcc cacaccgccc tccggacacg cctgaagcga agtttggcca
     8821 tcgcttataa gagtcacata ataaaagcct tatattattt gcttcggatg ggatgatccg
     8881 cccgctttgg cttcccactt gcctttctat atgtgaaagg agaactacaa tgaccgtact
     8941 tctcgtgctg ttcacgtttc tggttttcat cgcagtggac tacatcctga cccgcaagaa
     9001 ggcagctcag ccgcacgccg cgccggacgt cgttgcttcg cctgtgctcg atcccgcgta
     9061 tgttgaggga ttcctcgtgc cggagaagct ccagtatcac ccgggccata gctgggcaat
     9121 gcgggagcga aaacatctgc tccgggtggg aatcgacgaa ttcggcgccg ctttgatggg
     9181 acgcatcgag aaaatcgagg ttcccaagcc cggtgtttgg atccgccagg ggcagaaggc
     9241 cttctcattc ttccgggacg gcgagaaaac cgaggtactg tcaccgaccg agggcgaggt
     9301 catcgaggtc aacaacgacc tgctgaacga cccgtctctc ctgcgcaagg atccgtacgg
     9361 ggcaggctgg ctgatgatgg ttcacgttcc cgacgaggaa acggtgaacc ggaacctgat
     9421 tcccaagaac atggttcgag ggtggatacg tgacgccgtt gagcggctgt acgcccggca
     9481 gcctcagttt gccggtgcgg tggcggccga cggcggccgt ccggcggacg acttgttcgc
     9541 cgccctccca ggcgagtcct ggaaggaact gaccggggaa ttcttcctgt caaagtaacc
     9601 agccccgccg attcagattg gccgctgaat tgcacatgaa aacggtgcaa ttcagacgac
     9661 agggtctgaa agcggtgttc tgcgtaaacg gagaacaaac atgaagcaga gagccgtcct
     9721 atacgacagt accctgtgcg tcggttgcag ggcttgcgaa gaggcgtgtg cacggaagtg
     9781 gaatcttccg tacaatgacg cggtcgcgtc gaaagagcag ctttccgaga ataagctgac
     9841 tacgatcgcg acgtatggcg aacgctatgc tcgccggatg tgcatgcact gcgcggagcc
     9901 gacgtgcgtg tcggtatgcc ccgtgggggc gtttcaaaag acgccgctcg ggcccgtcat
     9961 ctatctggag gaccgttgca tgggctgccg gtattgcatg ctggcctgtc catacagcgt
    10021 acctgtctac gagtggagca agacgcttcc taaggtgagg aagtgcgaca tgtgctacga
    10081 ccgcgtgagg gcgggtaagc cgacgagttg ctcggaagcc tgcccgaccg gcgcgacgat
    10141 caccggcgaa cggaacgcat tgatcaaaga agcacagcgg cggatgacgg agaatcccag
    10201 ccagtacaat ggccgcatct acggcctgga agaagtgggc gggacttcgg tcctgatgct
    10261 ggccggtgtg ccgatggagc agttgggagt tcgagcgaat cttcccaatg cacccctatc
    10321 gctcgccacc cacgcagcgt tgtcgtttgt gcctgacatc gtgggggtcg gcgctgtctt
    10381 gctctcgggt gtctattgga tcactcaccg ccgcgaggaa gtcgccgcgg ccgagggcgg
    10441 tccccaaaag ggaagggaga agacgaaatg accaggcttc tccggttgcc tcggctgaca
    10501 ttctggcaat tggtgttcgc tctcctgatg cttgtcggcg cttacgcgac ctacgtgcgg
    10561 ttttttcacg gcctcggcgc ggccacaaac ttgaacgacc ggttcccgtg gggaatctgg
    10621 atcggattcg acgtcttgtg cggggtgggc ctggcggcgg gcggctttac acttgcggcc
    10681 acggttcaca ttttcaacat cgagaagtac aagccaatcg tacggccggc gattcttacc
    10741 gccttcctcg gctacactct ggtgatcctc gccctcttgt tcgacctcgg ccggccctat
    10801 cgcgtttggc atccgctggt gatgtggaat ccgcattcgg tgatgttcga ggtcggctgg
    10861 tgcgtgacac tctacacgac cgtgctggcg tttgagttcg ctccgatggt gctggaacgt
    10921 atcggctggc acgccggatt gaagtgggtt cgatccgcca tgatcccgct ggtgatcgcg
    10981 ggcgtcatat tgtcgactct gcaccagtcg tcactcggca gcctctatct gatcgttccg
    11041 cacaaactcc acccgctctg gtacactccg ctgctgccga ttctcttctt tgtctccgcg
    11101 atttgcgtcg gcctggcgat gaccattttc gagtcgtggc attccgcgaa ggcctgtgga
    11161 aggtacctcg aactgcgctt gctggctggg ctttcgcggg tgctggcggt tctgctgctg
    11221 gcatacctgg cgttgaggtt cctggatctg gcgcgacggg gcgcgctacg gttaattctg
    11281 gaacagcgga tggagagtta cctgttcggg ctcgagatcg cgctgatgct gcttccgatg
    11341 ctgctgcttt tccgggcgca cgtccgccgc agggcgggag cgctttacgc ctgctcggtg
    11401 atggtgattt tcggtttcat cacgcaccgc ctgaacgtca gcgttaccgg catggaggcg
    11461 ggttccgggg tgcaatactt gccaaaatgg acggaaattg cgatcaccct ggccatcgtg
    11521 gcagcagggt tcgctatttt tcgattcgcc acgaagtacc tgccgatctt cggagaggag
    11581 gagcagcaca gcgaagttgt agctgtcgcg ccagaggcgc tgccatctcc ggtgcggggg
    11641 gattgagaag taggagggaa gaacgggcca gaacggccat tctcgaatcg gcctcgccgt
    11701 gaagctcgcc gtctgcgtgg tggcgagttt cacggcgttt gtcgctttgt tcagctacct
    11761 gggtttccgc gagcatcggc ggcattccga ggatctggta gtgaccagcg cggaccgcct
    11821 taccgacgtg atccagcgca gcaccagcta ccagatgctg cacaatgacc gtgcggcgct
    11881 ttaccagatg atccatgcca tcggcagcga gccgggcata cgccgggtgc gcatcttcaa
    11941 tgaggatggg cgcatcagct tctccacttc cgagaccgaa gtgggaacgg tgctcgacaa
    12001 acaggcagag gcctgttacg cctgccacgc ccaggcagcg ccgctgacta agctggaccg
    12061 gccggatcgt gcgcgcattt ttccggagga gaacgcaaag cgcgtgctcg gcataatccg
    12121 gccgatcgag aatcaaccgg cctgttcgaa cgccgcctgc catgcccatc cgccccagcg
    12181 gcggatcctt ggcgtcaccg acgtgcaaat atccctggag agcgtcgacg cgcaactggc
    12241 ggagtaccgg cgccatctgc tggccatcac aggcctgtcg gtgctgttgg gttcgctggt
    12301 cagcgttggc ttcattctgt tgtttgtaca caggcccgtc ggggaattga tcgccggcac
    12361 ccaaacggtg gccggcggga atcttggtca ccggttgccc gtgcggtcag gagacgaact
    12421 gggagagttg gccgaatcat tcaacaagat gacggaggat ctggagaggg cccatttgga
    12481 gatcaccacc tgggctcgta cgctcgagga gcgggtggag aaaaagtccg ctgagttgca
    12541 gcgcgtccac gagagtctgc tgacaagcga aaagatggca tcgcttggaa agctggcggc
    12601 cacggtcgcc cacgaagtaa acaacccgct gttcgggatt ctgacctacg cccgcctgtc
    12661 gctccgggct ctcgagaaga gtgatctcag cccggagagc aaagcgcagg ttctcgatca
    12721 gttgcgcact atcgagaggg agagccggcg gtgcggcgaa atcatgaaga acctcctcaa
    12781 tttcgcgcgg caagtgccgc cgcaccggga agcgcatgac ctgaacgtgc tcgtcgacag
    12841 ggccatcact ctcgtccggc accaactggc gcttcaggag atcgagcttg agaaacgcct
    12901 tcaaggcgac ctgccggcct gcttctgtga cgcggaccag atccagcaag tggtgctggc
    12961 gttgttgatc aacgcatcgg atgtgatgcc gggaggaggg accattcgag tgacgacgga
    13021 gtcggtcaaa agcggggaaa acgcctgtat tcgagtagcc gacaccgggg gaggcatcga
    13081 ctcagagact ctgccgaaga ttttcgagcc gttcttcacc actaaggaag accagcaccg
    13141 caccgggctc ggcctggcca tcgcgaaagg tattgtcgag aggcacggcg gattcataac
    13201 cgtaatctcg aagcccggag agggcacgga attcaccatc ctgctgccgg ttggggagcc
    13261 tatcgcagcc ggtccggatg gaaaggggaa ccggaattga gtgtaccgga gacgaagtcc
    13321 agttctctcg gccgcatcct ggtcgtcgat gacgagctgg tggttcgtga ctccctcgac
    13381 aagtggtttg cgggagaggg ttacgaaacc ggcgttgcgg cgagcgcccg cgaggcgctg
    13441 gagatgttcc agaagaatga atgggatctt gtcctgctcg acatcaagat gcccgggatg
    13501 gacgggatgg agctgcaggc ccgcctgcgc gagattgacc cggaactggt catcatcatc
    13561 atgaccggct acgcttcggt ggagaccgcc gttcaggcac tgaagcgcgg cgcgtacgat
    13621 tacgtgacca agccgatcga cccggatgag ctggtccacc tggtctcgaa cgcgatcggg
    13681 caccggcggg accggcggga gatcgtccgg ctccgtgaga acctccagga gatctaccct
    13741 gagacgcacc ttatcggcaa cagtccgccg atgaagcacg tgctggaact ggtcgagatg
    13801 gttgcgccca ccgacacaac ggtgctgatc tctggcgaga gcgggaccgg caaggagctg
    13861 gtggcgcgag cgattcacgc ggccgggtcg cgccgctaca tgccgatggt ggtcatccat
    13921 tgcggcgcgc ttaccgagac gctgctcgag agcgagctgt tcggtcacga gcgaggcgcc
    13981 tttaccggcg cccagtaccg gaagaagggt aagttcgagg ttgccgatgg tggtacggtc
    14041 ttcctcgacg agatcagcga catcagcctc aagacgcaga ccgatctgct gcgtgtgctg
    14101 caggaaaagg agattgttcg tgtcggcgga aaccagtcga tcaaggtgga tttccgctgt
    14161 atcgccgcaa cgaacaagag tctcgaagag ctggtgaaag ccggcacgtt ccggccggat
    14221 cttttctacc ggctgaaggt tttctcgatc gaacttccac cgctccgaga ccggcgggag
    14281 gacatcccgc tgctcgcgga ccacttcata aagaaatacg ccaccgctat gaaccggcct
    14341 gagccgcgat tgtcgccgga agcgctcgac cttctactga gtcacaactg gccgggaaac
    14401 gtccgtgagt tggagaacgc gatcgagcgg gcgctggtga tcgggcgaga ggggctgatc
    14461 cagccttccg acttcccgtt ccaagtgcat tcgcagtccc cgcaagaggg ccggacactg
    14521 gaagacgtcg agagggtcca catcgaacgg gtccttcgcg agaccgcggg caatctctcc
    14581 cgtaccgcgc ggatcctcga catcgaccgg accacgctct acaacaagct caagcgatac
    14641 ggcctcaagt gatctccctg ccgcgcagaa tcgcagcgat tcatctgcag ccggttgggg
    14701 atggggtcac gctggagatg ctcgaccgga tatgcgcggc gctcgcgcgc ttattcgagg
    14761 tctcctgtca cgtacgcggc gagcggctcg acgccggact cgcgctcgat cctgtccgcc
    14821 tgcaatactt ctcaaccgcc atcctgcagc aactcgtgcc gctggcggcc ggagacgagg
    14881 agatccggct gctcggcatc accgagtacg atctattcgt gccggtgctg acgttcgtgt
    14941 tcggcgaggc gcaactggac gggcgctgcg ggctggtttc cctccacagg ttgagggagg
    15001 agttctacgg catgccggcg agcgaagaac ttgtggagga acgcgcggtg aaggaagtga
    15061 tccatgaact tgggcatacc ttcgggctca ggcactgtcc cgactggagt tgcgcgatgg
    15121 cgtcaaccca cgccgtggag cgcctggacc tcaaggaagc ccggctctgc gcggactgca
    15181 cgcggcgtct cggggcgtgg tcataaggaa ccgcgaactc aaaccgagcc gcaaccgcag
    15241 ggagcggacg cctgctatcg gcgaaagagc ttgtgcttga tgtccggcca gaattcgcga
    15301 aacacgttct tgccaagctc tatgccgaga gtcgtactgc cgcgcgcaag gccgtgccga
    15361 aagtcgttcc gcgagtcggg gtaccaggtg ttggcgagga acccagagcc gtaggcgccg
    15421 gcgaaacggc cgactgcaaa cgtctggccg ccattctcgg tccgccgcac aaaagtgcgt
    15481 ttcactgcgt gctctgtgcg gggccagaag ccgctgcact cgcagtggtc gtaagcaagc
    15541 acctcgcccc gcaaactcgc cacgccgagg tctatcgagt ttctggcgac tctgcgcgcg
    15601 tatgcggatg cgagccgccg ctcgaagccg tcgccaccct gttcccattc cggcgggctg
    15661 ttgcgccatt ggttccaccc cgctgtgacg gcggaaccca cgtatgccgc gggaccgaag
    15721 gtctgtttga tgtagtactg aaggttgctg tcaggatgag acgacgggat agcgctcaga
    15781 taactcgggg cggccgaatc ctgaccggtg gcggtgacaa cgaagaatcc cagtaacgcg
    15841 acgagtacta tcggcacatg gcctcagggc cagcccaaag ggctgttcac ttgatt
//
LOCUS       DATIFQ010000292        24764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2200476, whole genome shotgun sequence.
ACCESSION   DATIFQ010000292 DATIFQ010000000
VERSION     DATIFQ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24764
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200476"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2338)
                     /locus_tag="VLE22_13715"
     CDS             complement(<1..2338)
                     /locus_tag="VLE22_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15509.1"
                     /translation="MRVFAAYFLGVSSLLVCARAEDLTQQALALEKSGEAAEARLLLQ
                     RAAQRSPADAQAQLAYAEYLDRYADPETRMAYERALSLLQPSDDGGHRAALSRRLAVL
                     SLAAGDRDATRRYAEQYRTAGGRGWEIPQWTDSAPSDVEQHWTEIPGPLRSFSRMAAV
                     SQDVTEEDILPAVARNVVTNGYQASSGNEALEQTEYLKLVVRYLSQARELEKLAGPDK
                     VIRIPTCESTETGELLRILGYRMRGGCGSEVVLETVNASRAFLTIDSGFPIAELEQDL
                     RTNRPFSYDFKPTRAAVIYGADYWFSGKEKQGEFIDMLLADPQLCRLYLGMSKLDPVT
                     AEELRKALPVQRLKVFAHVLDFFGAMFEIRGGKAVVPGGARTAKAWEELTGVPRDQGP
                     AFFERLLAKDDGWMASYFDALARIQGPVKEYLTEPERLKRFYLAIRGRVTSPGPARPV
                     FRSNTELILLTNRLRIEPDGRPHIPGGIDVWRNLFIKHPHGKYDGKLTRLAATWKEPD
                     DVIEALFALCRKSVENEPLKIFMSISDINRHRTRVLETPTVERLILEYPELGAQYSVF
                     TEAPSLSDRTIVQYLDTIETVNRIRDTMLRANSAGTMQALVALWQIFVRQGSIPASAA
                     DETLAGVLTAFGKLREPEELFDAGRTGVSVLLKASGVPESEAPQDRMIDLLAGTRNPS
                     DPGAHNRVVEEMLRISEAQRLVPLTTLFDLAAHMEGLARGERLNTALVNRFASRTAEI
                     ESPRASLTATEKNAFAFGYWTERHIENQRKLNLRATLER"
     gene            complement(2467..3318)
                     /locus_tag="VLE22_13720"
     CDS             complement(2467..3318)
                     /locus_tag="VLE22_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB15510.1"
                     /translation="MVRKTFGWTGVEVPVIGQGTWMIEGTREAEQRAIEALRLGLDLG
                     MTHIDTAEMYGSGRAEELVAEAIAGRRNEVFLVSKVLPSNASYQGTLRACEKSLRRLK
                     TEWLDLYLLHWPGNHPIHETMRAMEDLAEAGKIRYLGVSNFDLDELQAARQALKRERI
                     ACDQVLYHLGDRGIERRLIPYCREHGIAVVGYSPFGHGGFPAERSAGGRVLAKIGSRY
                     GRTGRQVALNFLTREPGVFTIPKAANPEHARENAAGAGWELSEEEAAAIERAFPKPDR
                     DTQLGVI"
     gene            3364..4428
                     /locus_tag="VLE22_13725"
     CDS             3364..4428
                     /locus_tag="VLE22_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15511.1"
                     /translation="MTRIATLFVLFLALPAFAQEYKIAVVGLIHSHVWGHLGKMVKNE
                     PARLVGIAETEPELIAEAKKRGATNVAFYDDYRKMLDEAKPDIVWAFIENNRHLEIVQ
                     ACAPRKIHVMFEKPLASTYKDALAIRRLAQQHGIQVMTNYQMAWWPSNYAAKAQADAG
                     AVGKVWRLRGIVGHGGPGGPTGLNKFFFSWLTDPVKNGAGALVDFGCYNALWSLWYLG
                     RPESVYTQVNQLRPEEFPKVEDSSTMVLNYRNGVGIFEGSWDLPRGFQDLEVFGRGGS
                     LYVTSGKVELRKGREAAQDVILNPLPPERSEPIAYMIHCLRNKKAMEGLVALDINVDV
                     TEILDAARTSVKTGKAVRLR"
     gene            complement(4432..5673)
                     /locus_tag="VLE22_13730"
     CDS             complement(4432..5673)
                     /locus_tag="VLE22_13730"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HSB15512.1"
                     /translation="MASIFPFRAYRYSKSAGRLDDLVTQPYDKISPQMQARYLSLSPH
                     NLVRIILGERFPADTGTDNVYTRAGKSLEEWIASGILAREPEPALFAYFQRFRVPDTG
                     ETLLRKGFIGLGAVEDYAAGVVHRHEHTLAGPKKDRLELLRHTRAHFGQIFMLYPDPR
                     REIDSLLDEAAQAPAAAVVTDEYGVEHTVWTITDPARVEAIRAAMAPRKLVIADGHHR
                     YETALAFRDEHPELKDARRVMMTFVNMHSPGLKILATHRLVHGVDAAGFLASASATFR
                     VEECGDLDALRSVWSRPDPARVRIGVAMEGDHRIHLLVHERTDDELDVEFLHRKVLGD
                     ILGIGEEAVRGEKNLRYARGADTAVEEVRHGQARIAFLLEPTSVEGVARIAFSGGVMP
                     QKSTDFYPKLLSGLTIYRLDK"
     gene            5840..6904
                     /locus_tag="VLE22_13735"
     CDS             5840..6904
                     /locus_tag="VLE22_13735"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HSB15513.1"
                     /translation="MNRRFHRPRERGFTLLAAGACLVTMIGMLGLAVDVGRMYIVRNE
                     IQAYTDAASLAGALELDGTSAGITRAQAAAAAATNRWNMGTEPFSGTQTDFSTTASGP
                     WEGSPNPATGVRFVRVRATATLPLYFIPAVSASRSSTINATAVAGQELRTSFSEGTLP
                     FSPFPHDAADPNFGFTVGQRYTLRWGANPKVGPNSCDGDNAETWVNLAEAGNASERGY
                     IEETSSAIIRSAIEQDYQTRPLAVGDIVSMTGGNKQTQRDSLIARVNQDTDNVAATFA
                     EYKANDTGNGRRLVGVPINTWHPEYRIIGFAAFFLLLPSEYPQGGNKSFCAEYVGPYV
                     QGSWHPGAGGPGGYVVRLVR"
     gene            6901..7371
                     /locus_tag="VLE22_13740"
     CDS             6901..7371
                     /locus_tag="VLE22_13740"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HSB15514.1"
                     /translation="MRQPGNRARWRDARRGNAMVEFALSFGFLFSVFAGVFQFGYVYF
                     LYNQLENAVRGGARYASLRAYDSATATPSDGFTAAVKNVVVYGNPVGTGAAIAPGLTP
                     EKVSIAVVMERNVPRKVTLSIVNYRLDAVVTSFTLNGKPKVSFPYMGRFAPPGA"
     gene            7375..7785
                     /locus_tag="VLE22_13745"
     CDS             7375..7785
                     /locus_tag="VLE22_13745"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HSB15515.1"
                     /translation="MRPRRTCRRGQSAVEAVLVLLVFLATLIGIFDLGQILFVHQTFV
                     ARVRSAARYGVLDPTNVAAIKNMAVFGNPDGGGAGIFGLTPAMVSVTRTGNGTSEDRL
                     VVSISGYPYRLFSPWIGKAFTGRAITASYPVETP"
     gene            complement(7807..9864)
                     /locus_tag="VLE22_13750"
     CDS             complement(7807..9864)
                     /locus_tag="VLE22_13750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HSB15516.1"
                     /translation="MGTTGGSPTDNPLLEIQFQIPFDRVRAEHVGPAIHSLLEGAAVG
                     RDALANQDGEPTFAGVMMALDALTERLDYSMGLVRHLESVATYPELRAAHNAVEPAVS
                     AFYSGIPLHGGLWNRLRRYSGTADAAALAGVRKRFLTKTIDSFRRHGAELDPAGKTRL
                     KEIDVELSKLTTKFSENVLDSTNAFELVLTDEKQLAGLPPSAVSAARQSAASKGVTGW
                     RFTLQAPSYTALMTYLDDAEVRRHVWWAYGLRATEAQYDNRALIGRVLELRREKAKLL
                     GYRNFADLVLEDRMAHSGDRAFEFLRDLKRKTERHFGRENQELQAFRRDLEGSGAPEL
                     QPWDIGYYAEKQRAALYAFDEEALRPYFPLERVVGGMFEIVRRLYGIHVTERSGAPVW
                     DPQTRYYAIHDEDGTLLGCFYADWYPRENKRGGAWMDSFITGGPSADGFRPHAGLICG
                     NVTPPLDSRPALLTHREVETIFHEFGHLLHHCLSNVEIRTLAGTSVAWDFVELPSQIM
                     ENWCWEREALDLFAGHWETGEPFPEDLFRKMKRARTFRAANAQMRQLGFGFVDLALHM
                     DYTPDDGDVVAYTRRILQEFVPATLPDDHAMIASFTHLFSSPVAYAAGYYSYKWAEVL
                     DADAFSRFRERGIFDRNAGLEFRDKILARGDGEDPAELYRRFMGRDPDPNALLERLGL
                     NGK"
     gene            9969..10949
                     /locus_tag="VLE22_13755"
     CDS             9969..10949
                     /locus_tag="VLE22_13755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15517.1"
                     /translation="MLRLFGFLLALCLSAPAAGPVMIVLHKGASSMGFYTPEGKVFAT
                     VPVGAHPHEMVLSADGRLAYVSDNGTMRIEEAGTGGNTVSIVDLVTREKIGEISLGKF
                     HRPHGLDLDHETGVLAVSTELPDQLVLVDVKTRRIVKTYDTKGKTSHMVAWGPGARWA
                     YVCNSSSANVAAIERATGRVKLIPAGERPEGSVLSHDGRFLYVVNRESHGITIIDTAK
                     NEAVGEIRTGKGPVRIALTPDGRTLVYALMHDRKVEFADAATRRVTGQVPLGGQPVSL
                     GLSSDGKLAFASAQDDDTVYVVSVGEKKVIRQIRTAKGAAPDPALQVQLP"
     gene            complement(10953..11759)
                     /gene="lgt"
                     /locus_tag="VLE22_13760"
     CDS             complement(10953..11759)
                     /gene="lgt"
                     /locus_tag="VLE22_13760"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HSB15518.1"
                     /translation="MYPQLVRIGDFFLPTYGLLVALAFLTALWLAARLARHAGIDAER
                     VVNLGVYCALAGIVGAKLLMFVFDFDYYVRNPGEIFSLSTLQSGGVFQGGLILALVTA
                     VVYMRRNKLPGFATADAFAPGIAAGHAIGRIGCFSAGCCWGTECHRPWAVTFTNPAAN
                     EMFGTPLRVPLHPTQLYEAAGEAVIFGVLYRYYFRQHRPGAVIGLYLVLYSTLRFVVE
                     FYRAHDQSNPLEGPFSATQWIALGLFAAGVWLLRRRAPAVAQPQKEKVRA"
     gene            complement(11761..12261)
                     /gene="lspA"
                     /locus_tag="VLE22_13765"
     CDS             complement(11761..12261)
                     /gene="lspA"
                     /locus_tag="VLE22_13765"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HSB15519.1"
                     /translation="MRTRFVPYGIAASVFLLDRVTKAVISNQVSPWDTYVVIPGFFNI
                     VHTENRGMAFGFLANADNEWQSFFLAGLTILILALLATMLWQPAGKGLAGGGRARLAM
                     SLVMGGALGNLYDRILSGAVTDFLDFYIADHHWPAFNVADSAISIGAGLLILDMLITR
                     QTQERD"
     gene            complement(12261..15083)
                     /gene="ileS"
                     /locus_tag="VLE22_13770"
     CDS             complement(12261..15083)
                     /gene="ileS"
                     /locus_tag="VLE22_13770"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HSB15520.1"
                     /translation="MQLKDVDLKKTVNLPRTDFAMKANLPLAEPKMLARWEEMNIYER
                     VRQARAGRPMYVLHDGPPYANGNIHLGTAFNKILKDFIVKSHSSAGFDAPYIPGWDCH
                     GLPIEIKVDAELGSRKAKMTPSEIRTACRKYAARFVELHRREFKRLGVFGRWADPYLT
                     MSASYEAVIAGAFVEFLDKGYVYKGLKPVHWCMRCRTALAEAEVEYENHSSPSIWVRF
                     RLASDPAKIDPALAGRKVYAVIWTTTPWTIPANMAIAYHPKFEYVAVEVRGEVYLIAR
                     DLLKATSEKCGWVDPKIVASFTGAKLEGAIFRHPFVERDSPGILADHVTLEQGTGAVH
                     TATGHGLEDYVVGQRYGIATYCPVDAGGRFFHADGAPGRLPEELIGQTVWDANPIVIG
                     ILRERNALLAEGTVEHSYPHCWRCHKPTIFRATEQWFIGMDRNDLRARAIEKVREIRW
                     LPEWGEERISGMIGTRPDWCISRQRVWGVPILAFYCESCGEPLTERKYLDRVVGLFAE
                     KTADVWYELSEAELLGSGARCAKCGGEKFRKGSDILDVWFDSGSSHLAVLTGANKLPW
                     PSDLYIEGPDQYRGWFHSSLLIGLGVKNGSPYRQCATHGWTLDGEGKAMSKSVGNIIE
                     PDEIVKKYGAEVLRLWVASVDFTEDVRISETILTRLSEAYRKLRNTFRYALGNLCDFD
                     PATDAVPAGEMLELDQWILMRAERLVERCTAWYEQLAFHKVYRAVYDFATIDLSSIYF
                     DVLKDRLYTSATRSRARRSAQTALYRLTHALLRLLAPLLTFTTEEAWGHLRRPEGEPD
                     SVHLTVFPEPRELTDGISVGQRERAANWERLMEVRDNVLKSLEAARQEKFIGAPLEAR
                     VHLKADASLFPLLREYAAELPALFIVSEVRLEDRSDAILSVHVERASGTKCERCWKYT
                     ADVGANPELPTVCAACASAVTEIAGY"
     gene            15155..16153
                     /locus_tag="VLE22_13775"
     CDS             15155..16153
                     /locus_tag="VLE22_13775"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing serine
                     protease family protein"
                     /protein_id="HSB15521.1"
                     /translation="MALFLAAVAFGAPEPAGVKVVGLDARGGEAATGSGFFVTAGGEA
                     IVSRRALLGAVTAEIRTVEGRRYQVKSVVADSVHAGLARVAVDPPVKDSPFIAVVEAE
                     PVPGETVTVAGTEARISAVRNVPGFGPVFRISGSVQPEDFGRLVVNSRGAFIGVVLWS
                     TSEPSEMTLAGSAETVHYMKSGSPKTLAEWNQLARSAPPVEAAYRDGLNRLFLDDLDA
                     ALERFESVLEKDRRFAEAWFHAGFVQGKLGRRKEKIDSYREAIRLKPEFAAVHYSLGV
                     SYALMGEGQLAVEEFKALEKLDSELAEKLEILLEALGHQGHGHGEGEKVKPPPKQV"
     gene            complement(16185..17039)
                     /locus_tag="VLE22_13780"
     CDS             complement(16185..17039)
                     /locus_tag="VLE22_13780"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSB15522.1"
                     /translation="MNYSRFGSCTRLFSCLVVTTAAVLPLAAQTIQFNSVAPNVIETR
                     LQKAPADNQARFDTLKKLFEEAGCKELSEQAVKGAAQPNVLCTLPGSSPASIVVGAHF
                     DKGKKGDGIIDNWAGAALLPSLYQSLSAHPRKVTFVFVGFSEKEKGLAGSKAYVKEMT
                     AEQRAGAKAMVNVDCVGLAATKLIIDKSDKNLVNWFARVAASVKVPIMGTNAAQGATK
                     DSDPFADKKIPTIDVHSVGQEQLSVPHSEKDTLAAVSMDDYKNTFKALSAYLAFLDAT
                     SEAGAGGQ"
     gene            complement(17128..19113)
                     /locus_tag="VLE22_13785"
     CDS             complement(17128..19113)
                     /locus_tag="VLE22_13785"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: protein motif:HMM:TIGR03432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HSB15523.1"
                     /translation="MASVSLRSILDSGDDSIFDVPSKAPGPRGSLPLTPEMLLARPSG
                     DVFGWTQNAGMGWDPASLGAKEFLLLSTHGGVRAPDGTPVALGYHTGHWEVNLLVEAA
                     AKELKSHGATPFAGCCSDPCDGRTQGTPGMFDSLPYRNDAATVFRRLIRSLPTRRGVA
                     GVATCDKGLPAMMMALAGTRDLPCVLVPGGVMLATENGEDTALVQSVGARYAHGMISL
                     EEASLAGCNACGSPGGGCQFLGTAATSQVVAEALGMSLPHSALAPSGQPIWLDMARRS
                     ARSLLALEERGRRMRDILTEASVRNALVTHAAFGGSTNLVLHLTAVAHAAGLRRPSRQ
                     DWAEINRRVPRLVDVLPNGPKNYPTVQVFLAGGVPEVMLHLRRAGLLDLNAMTVAGDT
                     LGTMLDWWENSERRFRLRALLKDRDGVDPDDVIMNPGTARRRGLTSTAVFPAGNLAPE
                     GSVIKSTAIDPSVVDKDGVYRKTGPARVFTSEPDAVAAIKNNGPDRIKEGDVLVLTGR
                     GPMGSGMEETYQITSALKYLEFGKHVAVLTDARFSGVSTGACIGHISPEGLAGGPIGK
                     LRDGDLIEIVIDRNRLEGSVNLVGADGRRFDAEEGARVLAERPPHPGLSPDPNLPADS
                     RLWAVLQDVSGGAWGGCVYDVETILKVLEAGKRALRA"
     gene            19218..19538
                     /locus_tag="VLE22_13790"
     CDS             19218..19538
                     /locus_tag="VLE22_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15524.1"
                     /translation="MIVRIWHGWTAPGDADAYEALLKDEIFVGIGARRIEGYRGIELL
                     RRDSAEEVEFITIMRFDSIASVRMFAGADYEVAVVPTAARALLSRFDSRSQHYELREA
                     RPTH"
     gene            19662..20162
                     /gene="coaD"
                     /locus_tag="VLE22_13795"
     CDS             19662..20162
                     /gene="coaD"
                     /locus_tag="VLE22_13795"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSB15525.1"
                     /translation="MADKQRAVVAIYPGSFDPITNGHLDLIARGARLFDRLIVAVLQN
                     TDKEPLFSVEERTEMLRETISLYPNVEVDSFNGLLVDYAARKNASVILRGIRAISDYE
                     YELQMALMNTRLSPGIETVFLMSSEAYSFISSRLVKQVFGLRGNISGLVPPVVERRLQ
                     SRAIEK"
     gene            20187..21374
                     /locus_tag="VLE22_13800"
     CDS             20187..21374
                     /locus_tag="VLE22_13800"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSB15526.1"
                     /translation="MLVTTELAERMSRISVSATMKVATDAEKLRAHGIDVVDFGAGEP
                     DFPTPDNIKQAAVRALEANFTKYTASGGTMELKEAVCERHALDFGTSYTPSECVVSVG
                     GKHVIFNLMQVLLNPGDEVIIPAPYWVTYKDVVHYAEGKCVFVPADEASGFEVTAAMI
                     EEHLTPKTKAVLINSPSNPAGAVIGREEFEHIYRLTSKRGVWLITDECYCHFLYDSAP
                     FSVASIPGARHSVIVASSLSKTYAMTGWRIGFALAPPPVVAAIAKLQSQSTSNPNSIA
                     QKAAVEALRGPQDSVDAMLAEYRHRRDFVIRRLRQIPGVTCAEPKGAFYAYPNIGAAI
                     GRNGIQDTLSFAEQVLDEARVAVVPGEAFGTAEHVRISYATSMHELERGLDRLHNFIT
                     NLG"
     gene            21374..22186
                     /locus_tag="VLE22_13805"
     CDS             21374..22186
                     /locus_tag="VLE22_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HSB15527.1"
                     /translation="MDLGPFRLIPSFREKVWGVTDLAPWFPQASGKIGEVWFLRPDDV
                     PQPILVKIIFTSALLSVQVHPHDAYALAHDGIPGKTEMWYILRAEPGAKLALGLREPV
                     TREALKEAAASGVVGQLLQWFPVSPGQVVFIPPGTVHAIGPGITLCEIQQNSDITYRL
                     YDYGRPRELHVDKALDVATLGPHPGPSAPAGATLAACDYFITEQIEIGRATAYTPDRD
                     RFHLLVFLEGAGTIAGQPFRAGEVWHVPEGAGTFSLDPDGPVRLLRSSPPHR"
     gene            22349..23410
                     /locus_tag="VLE22_13810"
     CDS             22349..23410
                     /locus_tag="VLE22_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15528.1"
                     /translation="MNRSRVLCILLPLFSLLAGCGSRRSGPPAIGEAYVGPATLNIRK
                     DLGLHRPTVATVKHGERLDIVGRRRAFVQVRTESGAIGWVDGRQLFTTEQMAELRSVA
                     DRAAALPSQGQASIQEALNVHTHPNRQSPSFRQLKTGERVDVVAHRLEPRVPFRPDRP
                     VQSLPPSAPMEDWSLVRTADGKAGWVLFRLLFMAIPDEVAQYAEGHRITSYFSLGEVH
                     DGDAVKHNWLWTTIARGLEPYEFDGFRVFVWSLRRHRYETAYIERNLRGYYPVELHPE
                     QGGAPSFSLIIEDKDGVRSKRTYAFQGYRVRLIATEPWNPGSDAPERIAGAEPPFQEQ
                     DQRSFFGRLWDKIKGIFGR"
     gene            complement(23414..>24764)
                     /locus_tag="VLE22_13815"
     CDS             complement(23414..>24764)
                     /locus_tag="VLE22_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HSB15529.1"
                     /translation="YLSGTIFLKSRVTLYLDAGAVLLGSRKLEHYPITVPAFRSYTDN
                     YTDKSLIYGENVEDIAIEGRGVIDGQGAAFQGPYKVRPYMIRIIQCRNVSVTGVTMKD
                     SPMWVQHYLACDGVAIRGITVHSRVNHNNDGIDIDCCERVRISDCDISSGDDAIVLKS
                     TADRPCKDVAVTNCVLSTACNAFKLGTETNGGFENIVFSNSTIYDTRLGGIAIECVDG
                     GVLDKVNVSNITMRNVGGPIFIRLGNRARPFVEGGQRPGIGRLRNVQISDVQITGGGS
                     VGCAISGIPEHPIENVTLSNVQISFAGGGEKEDPGKEVPEVPEKYPEFAMFGKLPAYG
                     FYCRHASNVAFRNVEAGFEKPDGRAAMICDDIEGLELAGTDLAVLPDAPCAVRLQNVR
                     DAFVHGCRARAGTSAWLSVAGTQSARIKLAANDLSRAKKAVELAAGAPPAAVSGAED"
BASE COUNT         4448 a         7984 c         7692 g         4640 t
ORIGIN      
        1 cccgctccag cgtggcacgc aagttcagct tgcgctggtt ctcgatgtgc cgctctgtcc
       61 agtagccgaa cgcgaaggca ttcttttccg tggcggtgag cgaagcccgc ggcgattcga
      121 tctcggcggt gcgcgacgca aaacgattca cgagagccgt gttgagccgt tcgcctctgg
      181 caagtccctc catgtgcgcg gccaagtcga acagcgtagt cagcgggacc agccgttgcg
      241 cttcagaaat gcgaagcatc tcctccacca cgcgattgtg ggcgccggga tccgatggat
      301 tccgcgttcc agccagaagg tcgatcatgc ggtcctgcgg agcctcgctt tccggcactc
      361 cgctcgcttt gagaagaacg gacaccccgg ttctgccggc atcgaagagt tcctccggct
      421 cccgcagttt tccaaacgca gtcaggactc ccgccagagt ctcatcggca gccgaagctg
      481 ggatcgaacc ctgccggacg aaaatctgcc agagcgccac cagcgcctgc atcgtacccg
      541 cggaatttgc ccgcaacatc gtgtcgcgga tgcggttgac ggtctcgatg gtgtccagat
      601 actgaacgat ggttcggtcg ctcagagagg gcgcttccgt gaaaacgcta tactgcgcgc
      661 cgagctcagg gtattcgagg atcagccgct ccaccgtcgg cgtttccaga acgcgggttc
      721 ggtgccggtt gatgtcgctg atggacatga aaatcttcag cggctcgttc tcgacggact
      781 tgcggcacag cgcgaacagc gcttcgatca cgtcatccgg ctctttccac gttgccgcga
      841 gccgcgtcag cttgccgtcg tattttccgt gcgggtgctt gatgaacagg ttcctccaga
      901 cgtcaatccc gcccgggatg tgcggtctgc catctggctc gatgcggagc cggttggtga
      961 gcaggatcag ttcggtgttc gagcggaata ccggtctggc cggccccgga ctggtgacgc
     1021 ggccacggat cgccagatag aaccgcttca accgttcggg ttccgtcagg tactccttca
     1081 ccggcccttg aatccgcgcc agggcgtcga agtagctcgc catccagccg tcgtccttcg
     1141 ccagcagacg ttcgaagaac gccggtccct ggtcccgggg cacgcccgtc aattcctccc
     1201 acgccttcgc ggtgcgggcg ccgcccggaa cgaccgcctt gccgccccga atctcgaaca
     1261 tcgccccgaa aaagtccagg acgtgcgcga aaaccttcag ccgctgcacc ggcagcgcct
     1321 tgcgcagttc ctcggcggtc accggatcga gtttcgacat tcccagataa agccggcaaa
     1381 gttgagggtc ggcaagaagc atgtcgatga actcgccctg cttctctttt ccggagaacc
     1441 agtagtcagc tccgtaaatg acggccgccc gggtcggctt gaaatcgtac gagaacggcc
     1501 ggttggtgcg aaggtcctgt tccagttcgg caatcgggaa acctgaatcg atggtcagga
     1561 acgcgcgcga cgcgttcacc gtctccagta cgacttcact cccgcagcct ccgcgcattc
     1621 ggtagccgag gatgcgcagg agttctccgg tctccgtcga ctcgcaggtc gggatgcgga
     1681 tcaccttgtc gggaccggcg agtttctcga gctcgcgcgc ctgggagaga taacggacca
     1741 ccagtttgag gtactcggtc tgttcgagcg cctcattgcc gctcgaggcc tggtagccgt
     1801 tcgtcacgac attgcgcgcc actgccggca ggatgtcctc ttccgttaca tcctgcgaga
     1861 cggcggccat cctggagaat gagcgaagag gcccgggaat ctcggtccag tgttgctcga
     1921 cgtccgaggg ggcggaatca gtccattgtg gaatctccca gccacggccc ccggctgtcc
     1981 gatactgctc ggcatagcgg cgggtggcat cacgatcccc ggctgccaac gacagcaccg
     2041 ccagacggcg gcttagtgct gcgcgatgtc caccgtcgtc cgatggctgg agaaggctga
     2101 gggccctctc gtacgccatt cgggtctctg gatccgcgta ccggtcgaga tattccgcat
     2161 aggccaactg ggcctgtgcg tcggcgggcg acctctgcgc cgcccgttgg agaaggaggc
     2221 gggcttccgc cgcctcgccg cttttctcga gcgcaagcgc ctgttgggtc aggtcctccg
     2281 ctcgtgcaca tacaagaagg ctagagacgc ccaggaaata cgccgcaaac accctcatgt
     2341 ccgtttctct cgtttgtaaa attcttgcca gccggttggt ttttactgta tcactttttc
     2401 cacaagctcg cagccggagg gatgttaaat catggcaacc tccaagcccg ctatgacagc
     2461 ggaactctat atcactccga gctgcgtgtc ccggtcgggt ttcgggaacg ctctctcgat
     2521 cgcggcggcc tcttcttcgc tgagctccca gccggctcca gcggcgttct cgcgcgcgtg
     2581 ttcgggattg gcggcttttg gaatcgtgaa gacgccgggc tcgcgggtga gaaagttcag
     2641 cgccacctgc cgccccgtgc ggccataacg ggagccgatt tttgccagca cgcggccccc
     2701 ggcgctgcgc tcggccggaa acccgccgtg cccgaacgga ctgtagccga cgactgcgat
     2761 cccgtgctca cggcagtaag ggatcaggcg gcgttcgatg ccacgatccc cgagatggta
     2821 gagcacctgg tcgcaggcga tgcgctcccg cttcaaagct tgccgggctg cctgcaactc
     2881 atcgagatcg aagttgctca cgccgaggta gcggatcttc cccgcttctg ccaggtcttc
     2941 catggcgcgc atcgtttcgt gaatcggatg gtttccaggc caatgaagca gatacagatc
     3001 gagccattcc gtcttcagcc ggcggaggct cttctcgcaa gcccgcaggg taccctggta
     3061 agaggcgttc gagggaagta ccttgctcac caggaagacc tcgttccggc gcccggcgat
     3121 ggcctcagcc accagttcct cggcacgccc gctgccatac atctcggcgg tgtcgatatg
     3181 cgtcatgccc aggtcgagcc ctagccgcag tgcctcgatc gcgcgctgct ccgcctcgcg
     3241 cgtgccctcg atcatccatg tcccctgacc gatcaccggg acctccaccc cggtccatcc
     3301 gaacgttttt cgcaccatct tttgaactat agcgctatgc tggggggcgg acagaagaaa
     3361 cccatgaccc gaatcgcaac tcttttcgtt ctctttctcg ccctgccggc gttcgcgcag
     3421 gagtacaaaa tcgctgtcgt cggattgatt cattctcatg tctggggtca tctcggcaag
     3481 atggtgaaga atgagccggc gcggctcgtt ggtatcgctg agacggaacc cgagctgatc
     3541 gccgaggcga agaagcgggg cgccacgaac gtcgccttct atgatgacta ccggaaaatg
     3601 ctggatgaag cgaagcccga tatcgtctgg gcgttcatcg agaacaaccg gcacctcgaa
     3661 atcgtccagg cgtgcgcgcc gcgaaagatc cacgtgatgt ttgagaagcc gctggcatcg
     3721 acgtacaagg acgcgcttgc cattcgccgt ctcgcacagc agcacggcat ccaggtgatg
     3781 acgaactacc agatggcctg gtggccctca aactacgccg ccaaggcgca ggcggacgcg
     3841 ggcgccgtcg gcaaagtctg gcgactgcgc ggcatcgtgg gacatggcgg tccgggaggt
     3901 cccaccggtc tcaacaagtt cttcttctcc tggctcacgg atccggtcaa gaacggagcg
     3961 ggcgcgctcg tcgatttcgg ctgctataac gcgctgtggt cactgtggta cctggggcgg
     4021 cccgaatcgg tgtacacaca ggtcaaccag cttcggcccg aggagtttcc caaggtcgag
     4081 gacagctcca cgatggtttt gaattacaga aacggcgtcg gaatcttcga ggggagctgg
     4141 gatctgccgc ggggtttcca ggatttggaa gtgttcggcc gcggaggcag cctctacgtg
     4201 accagcggta aagtggaact gcgcaagggg agggaagcgg cgcaggacgt catcctcaat
     4261 ccgctgccgc ccgagcggtc cgaaccgatc gcttacatga tccactgcct gcggaacaag
     4321 aaggcgatgg agggccttgt ggcgctcgac atcaacgtcg acgtgacgga gattcttgac
     4381 gccgcccgca cgtcggtgaa gaccggcaag gccgtgcgtt tgcgatagcg cctatttgtc
     4441 cagcctataa atggtcaggc cggaaagcag tttcggatag aagtcggtgg atttctgtgg
     4501 catgacgccg cccgagaacg cgatccgcgc aacgccctcg accgacgtcg gctcaagcag
     4561 gaatgcgatc cgggcctggc cgtggcgcac ctcctcgacc gcggtatcgg cgccgcgagc
     4621 gtagcggagg tttttctcgc cgcgcaccgc ttcttcgccg attccgagga tatcgcccag
     4681 caccttccgg tgcaggaatt ccacgtcgag ctcgtcatcc gtccgctcgt gtacgagcag
     4741 atgaatccgg tgatcgcctt ccatcgcgac gccgatgcgt accctggccg ggtccggacg
     4801 gctccagacg cttcgcagcg cgtcgagatc cccgcactct tccacccgga acgtcgcgct
     4861 ggcgcttgcc agaaagcctg ccgcatcgac accgtgaacg aggcgatgtg tcgccaggat
     4921 cttcagccca ggcgagtgca tattgacgaa ggtcatcatc accctgcggg cgtctttgag
     4981 ctccgggtgc tcgtcccgaa aggcaagcgc cgtctcgtag cggtggtggc cgtcagcgat
     5041 caccagtttt cggggcgcca tcgccgcccg gatcgcctct acgcgtgccg ggtcggtgat
     5101 cgtccagacc gtgtgctcga cgccgtattc gtccgttacc acggccgccg ccggcgcttg
     5161 tgcagcctcg tcgagtagcg aatcgatttc cctcctcggg tcgggatata gcatgaagat
     5221 ctggccgaaa tgcgcgcgcg tgtgccgcaa caattcgagc cggtccttct tcggtccggc
     5281 gagggtgtgc tcgtgcctgt gaaccactcc cgccgcgtag tcctccaccg ctcccagtcc
     5341 gatgaaaccc ttccgcagta gcgtctcgcc agtgtcgggc acgcggaagc gctggaagta
     5401 cgcaaatagc gccggttcag gctcgcgcgc caggattccg gatgcgatcc actcttcgag
     5461 agacttgccg gcccgcgtgt acacgttgtc ggtcccggtg tcggccggga atcgctcacc
     5521 gagaatgatg cggaccagat tgtgcgggct cagcgaaaga taccgcgcct gcatctgagg
     5581 gctgatcttg tcgtaaggct gcgtaaccag gtcgtccagc cgccccgccg atttgctgta
     5641 acggtacgca cgaaacggaa aaatgctcgc catgggaagg agagatttta ggataacaga
     5701 gggcgggatt cgagaaacca aaaccctcta cgtgtttccc cttatccccg cgacagagct
     5761 ttttcgcatc cgtctcggtc acttttcaag gcggctgaag tcggcgcagg caatagactt
     5821 cgaagtagag gcaaaaacga tgaaccgtcg cttccatcgc cctcgggaac ggggcttcac
     5881 gctgcttgcg gcgggggcct gcctggtgac gatgatcggc atgctcggcc tggcggttga
     5941 cgtcggccgc atgtacatcg tgcggaacga gatccaggcc tacacagacg ccgcgtccct
     6001 ggccggcgcg ctcgagttgg atggcacgtc cgcgggcatc acacgggcgc aggccgccgc
     6061 cgcggcggcc accaaccgtt ggaatatggg caccgagccc ttcagcggaa cccagacaga
     6121 cttctcgaca acggcctccg ggccatggga gggaagcccc aatcccgcta cgggcgtccg
     6181 cttcgtccgt gtccgcgcca cggcgaccct gccgttgtac ttcatccccg cggtgtcggc
     6241 atcccgttcg tccaccatta acgcgactgc ggtcgccgga caggaactca gaaccagctt
     6301 cagcgagggt actcttcctt tctcgccctt cccacacgat gccgcagatc cgaacttcgg
     6361 attcacggtc ggccagcgct acacgctgcg ctggggagcg aatcccaaag tcggcccgaa
     6421 cagttgcgat ggagacaacg cggagacctg ggtgaatctg gccgaggccg gcaacgcctc
     6481 cgagcgcggc tacattgagg agacgagctc ggccatcatc cgctctgcaa tcgagcagga
     6541 ctatcagacc aggcccctgg ccgtaggcga catcgtaagc atgaccggcg ggaacaagca
     6601 gacgcagcgc gacagcctga tcgcccgcgt gaaccaggac accgataacg tcgccgccac
     6661 attcgcggaa tacaaggcta acgacaccgg aaacgggcgg aggctggtcg gagtcccgat
     6721 caacacatgg catcccgagt accgcatcat cggattcgcc gcgtttttcc tgcttttgcc
     6781 gtcggaatac ccccagggcg gcaacaagtc gttctgcgcc gaatacgtcg gaccgtacgt
     6841 ccagggaagc tggcatccgg gcgctggcgg ccccgggggt tacgttgtga ggctggtgcg
     6901 atgaggcagc ccggcaaccg ggcgcggtgg cgcgacgccc gccgcgggaa cgcgatggtg
     6961 gagttcgccc tgtccttcgg atttctcttc tcggtgtttg cgggcgtttt tcagttcggc
     7021 tacgtctact tcctttacaa ccagctcgaa aacgccgttc gcggcggcgc gcgctatgct
     7081 tccctgcgcg cttacgattc ggccaccgcc acgccgtcgg acggcttcac cgccgccgtg
     7141 aagaacgtcg tggtatacgg aaaccccgtt ggcacgggcg cggcgatcgc ccccggactg
     7201 acgccggaga aggtctccat cgccgtagtg atggagcgca acgtgccgcg caaggtcacg
     7261 ttgtcgatcg tcaactaccg gctcgacgcc gtggtcacat cgttcaccct gaacggaaaa
     7321 ccgaaggtca gctttccgta catgggccgc ttcgcaccgc ccggagcgtg agacatgaga
     7381 ccacgacgca cgtgccgcag gggacagagc gccgtcgagg cggttctcgt ccttcttgtg
     7441 ttcctcgcta ccctcatcgg gatattcgac ctcggccaga ttctgttcgt ccaccagacg
     7501 tttgtggcgc gcgtgcggag cgccgcgcgc tacggcgttc tggacccaac taatgtcgct
     7561 gccatcaaga acatggctgt tttcggcaat ccggacggcg gcggcgctgg aatcttcggg
     7621 ctgacgcccg cgatggtttc cgtcacccgc accggtaacg gcaccagcga agaccgcctg
     7681 gtcgtcagca tcagcggata tccgtaccgc ctgttctcgc cctggatcgg caaagcgttc
     7741 accggacggg caatcactgc cagctacccc gtcgagacgc cgtgagtgtt gtcgcgcggc
     7801 ataccgctat ttcccgttga ggcccagccg ttcgaggagc gcgttcggat cgggatcgcg
     7861 gcccatgaag cgtcggtaga gttccgccgg gtcctcgccg tctccccgcg ccaggatctt
     7921 gtcgcgaaac tccaagccgg cgtttcggtc gaaaatccca cgctcccgga agcggctgaa
     7981 cgcatcggcg tcgagcactt ccgcccactt gtaggagtag taccccgcgg cgtacgccac
     8041 cggacttgaa aacaagtgcg tgaacgacgc gatcatcgca tgatcgtccg gcagcgtcgc
     8101 gggaacaaat tcctggagga tccgccgggt gtaggcgacg acgtcgccgt cgtccggcgt
     8161 gtagtccatg tggagtgcca ggtcgacgaa gccgaagccg agttgccgca tctgtgcgtt
     8221 tgccgcgcga aacgtcctcg cgcgcttcat tttgcggaac agatcctcgg ggaacggctc
     8281 gccggtttcc cagtggcccg cgaacaggtc cagcgcctcc cgctcccagc accagttctc
     8341 catgatctgc gacggcagtt cgacaaaatc ccatgccacg ctcgtgccgg cgagtgtacg
     8401 gatctccacg ttgctaagac agtgatgcag cagatgaccg aactcgtgga agatcgtttc
     8461 gacctcgcgg tgcgtgagca gcgccggccg gctgtcgagc ggcggagtga cgttaccgca
     8521 gatcagcccg gcgtgaggcc ggaaaccgtc cgcgcttggc cctcccgtaa taaacgagtc
     8581 catccaggcg ccaccacgct tgttttcccg cgggtaccag tcggcataga aacagccgag
     8641 caacgtgccg tcctcgtcgt gaatggcgta gtagcgtgtc tgcgggtccc acacgggcgc
     8701 acccgaccgc tcggtcacgt ggatcccgta cagccggcgg acgatttcga acatgcctcc
     8761 gacgacgcgc tccagcggga agtacggacg cagcgcctcc tcgtcgaagg catagagggc
     8821 ggccctctgc ttctccgcgt agtaaccgat gtcccagggc tgtagttccg gtgcgccgga
     8881 accctccaga tcccgacgaa aggcctgcaa ctcctgattt tcccggccga agtgccgctc
     8941 cgtcttgcgc ttcaggtcgc gcaggaactc gaaggcgcgg tcgccgctgt gcgccatccg
     9001 atcttccagc acgagatcgg caaagttccg gtaaccgagc agcttggcct tttcgcgcct
     9061 gagttcgagc acacggccga tgagcgcccg gttgtcgtac tgggcttccg tggcccgaag
     9121 tccgtatgcc caccacacgt gcctgcggac ttcggcgtcg tccaggtagg tcatcagcgc
     9181 cgtgtaactt ggagcctgga gcgtgaagcg ccagccggtt acgcccttcg atgcggcgct
     9241 ctggcgggcg gcggaaaccg cggacggcgg cagcccggcc agttgtttct cgtccgtcag
     9301 caccagctcg aaggcgttgg tcgagtcgag cacgttctcc gagaacttcg tcgtcagctt
     9361 gctcaactcg acgtcgatct ccttgagcct cgtctttccc gccggatcga gttcggcgcc
     9421 gtgccgccgg aaactgtcga ttgtctttgt tagaaaccgt ttccgcacgc cggccagcgc
     9481 agccgcatcg gcggtcccgg agtaccgccg cagacggttc cacaggcctc catgcagcgg
     9541 gatgccggaa tagaaggcgc ttaccgccgg ctcgacggcg ttgtgcgctg cgcgaagttc
     9601 gggatacgtg gcgacggact ccagatgccg gacgagaccc atggagtagt cgaggcgttc
     9661 ggtcagtgca tccagcgcca tcatgacgcc tgcgaaggtc ggttctccgt cctggttcgc
     9721 tagtgcatcc ctgcccacag ccgccccctc gagcaggctg tgaatcgccg gtccgacgtg
     9781 ctcggcgcgg acgcggtcga agggaatctg aaactggatc tccagcaggg ggttgtctgt
     9841 gggcgagccg ccggttgttc ccatctctcc attatcgcgg acgccagccg gcggcctttc
     9901 cattgtcacg tctggcggat agactgtaat gcgacgttgt aagccagaac ccgaggaggc
     9961 tgtgtaccat gctccggctt tttggttttc ttctcgctct ctgcctaagc gcccccgccg
    10021 ccgggccggt gatgatcgtt ctccacaagg gcgccagctc gatgggcttc tataccccgg
    10081 aaggcaaggt gttcgccact gttcccgtcg gcgcacatcc gcacgagatg gttctgtcgg
    10141 ccgacggacg cctggcgtac gtctcggaca acggaaccat gcggattgaa gaggccggta
    10201 ccggcggcaa caccgtgtcg atcgtcgatc tcgtcacgcg ggaaaagatc ggcgagattt
    10261 cgctcggaaa attccaccgc ccgcacggtc tcgaccttga ccacgaaacc ggcgtgctgg
    10321 ccgtctcgac cgaactgccc gatcagttgg tcctcgtcga tgtaaagacc cggcggatcg
    10381 tgaagaccta cgacacgaag ggcaagacct ctcacatggt cgcctggggt cccggcgcgc
    10441 gttgggctta cgtgtgtaac agcagttccg cgaacgtcgc cgcgatcgag cgcgccaccg
    10501 gcagggtgaa gctgattccg gcgggcgagc ggccggaggg aagcgtcctg tctcacgacg
    10561 gccgtttcct ttacgtggtg aaccgtgaat cacatggcat taccatcatc gacacggcga
    10621 agaacgaggc ggtgggtgag atccgaaccg ggaaaggtcc ggtccgcatc gctctcacgc
    10681 cggacggccg gacgctcgtg tatgccctga tgcacgaccg caaggttgag tttgccgacg
    10741 ccgccacaag gcgcgtcacc gggcaggttc cgctgggcgg ccagcccgta tcgctcgggc
    10801 tgtcatccga cgggaaactc gcgttcgcct ccgcgcagga cgatgacact gtctacgtcg
    10861 tgtcggtggg ggagaagaaa gtgatccggc agatcaggac ggcgaaggga gcggctccgg
    10921 atccggccct acaggtccag ttgccatagc cgctacgcgc gaactttttc tttctgcggc
    10981 tgcgctacgg cgggcgcgcg ccgccggagc aaccagacgc cggctgcgaa caggccgagc
    11041 gcgatccatt gcgtcgcgga gaacggcccc tccagcggat tcgactggtc gtgcgctcgg
    11101 tagaactcaa caacgaaacg aagcgtcgag tacaggacga ggtacagccc gatgacagcc
    11161 cctggacggt gctggcggaa atagtagcgg tagagcacac caaagatcac agcctcgccg
    11221 gccgcttcat acagttgagt tgggtgcagc ggcacccgta gcggcgtgcc gaacatctcg
    11281 tttgccgccg gattggtgaa ggtgaccgcc cacgggcgat ggcactcggt tccccagcag
    11341 catcccgcgg aaaaacatcc gatccggccg atggcgtgac cggccgcgat gccaggagcg
    11401 aatgcgtcgg cggtcgcgaa ccccggcagc ttgttccgcc gcatgtagac gaccgccgtg
    11461 accagtgcca ggatcaggcc tccctggaag actcctcccg attgcagggt ggacagggaa
    11521 aagatttcgc ctggattgcg cacgtagtaa tcgaagtcaa agacgaacat cagcagcttt
    11581 gctccgacaa ttccggccag cgcgcagtag acaccgaggt tgaccacccg ctcggcatcg
    11641 ataccggcat ggcgcgcgag cctcgccgcc agccacaggg ccgtcagaaa tgccagcgcc
    11701 accagcaggc cgtaagtcgg caggaaaaag tcaccgatgc ggactagttg cgggtacatc
    11761 tcaatcccgc tcctgtgttt gtctcgtgat cagcatgtca agaatcagaa gacccgcgcc
    11821 gatcgaaatc gcactgtcgg cgacgttgaa ggcgggccag tgatggtccg cgatgtagaa
    11881 atccagaaag tcggtaaccg cgccgctcag aatgcggtca tagaggtttc cgagcgcccc
    11941 gcccattact agcgacatcg ccagcctggc cctgccgcct cccgccaaac cctttccggc
    12001 gggctgccac agcatcgtgg ccagcagcgc caggatcaaa attgtcagac cggcgagaaa
    12061 gaagctctgc cattcgttgt cggcgttcgc cagaaacccg aacgccatac cgcgattctc
    12121 ggtgtgaacg atgttgaaaa accccggaat caccacatag gtgtcccacg gagacacctg
    12181 attgctgatc acggcctttg tgacgcgatc gagcaaaaat acactggccg cgatgccata
    12241 cgggacgaat cgggttcgca tcagtagccc gcaatctccg tcactgccga cgcgcaggcc
    12301 gcgcagaccg tcggcagttc cgggttggcg cccacgtccg ccgtgtactt ccagcagcgc
    12361 tcgcacttcg tgccggacgc ccgctccacg tgcaccgaca ggatcgcgtc gctccggtct
    12421 tccaggcgga cctcggaaac gatgaacaag gccggcagtt ccgcggcgta ctcacggagc
    12481 agcgggaaga gactggcgtc cgccttcaag tgaacgcgag cttccagcgg cgcgccgatg
    12541 aatttctcct gccgcgccgc ttccaggctc ttcaggacgt tgtcccgcac ctccatcagt
    12601 cgctcccaat tggccgcccg ttcacgctgc ccgacggaaa ttccgtcagt gagctcccgc
    12661 ggttcgggga acaccgtcag gtgcacgcta tccggttcgc cctccggcct gcgaaggtgg
    12721 ccccaggcct cctcggtggt gaaggtcagc aggggcgcca ggagccgcag caacgcgtgc
    12781 gtgagccggt aaagcgcggt ctgggcgctt cgcctcgcgc gggaacgggt cgcggatgtg
    12841 tacagccggt ctttcagcac gtcgaagtag atcgagctga ggtcgatggt ggcgaagtcg
    12901 taaaccgccc tgtacacttt atgaaacgcg agctgttcgt accacgccgt gcagcgctct
    12961 acgagcctct ccgcccgcat gaggatccat tgatccagtt cgagcatctc gccggcgggc
    13021 acggcatcag tggccggatc aaagtcgcac agattgccga gcgcataccg gaatgtgttg
    13081 cgcagcttcc ggtaggcttc cgacagacgg gtgaggattg tctccgagat ccttacatcc
    13141 tcggtgaaat caaccgaggc cacccacagg cgcaggactt cggcgccgta cttcttgacg
    13201 atctcgtccg gctcgatgat gttgccgacc gacttcgaca tcgctttgcc ctcgccgtcg
    13261 agggtccaac cgtgagtggc gcactgccgg tagggcgagc cgttcttcac gcccagtccg
    13321 atcaggagcg agctatggaa ccagccccgg tactggtccg gtccttcgat atacagatcc
    13381 gaaggccatg gcagtttgtt ggcgccggtc aggaccgcca gatggctgga acccgaatcg
    13441 aaccagacgt cgagaatatc gctgcccttc cggaatttct cgccgccgca tttcgcgcac
    13501 cgcgctccgg agcccagcag ctcggcttcg ctcagctcgt accacacgtc ggccgttttt
    13561 tcggcgaaca gcccgaccac gcggtccagg tacttccgtt cggtgagcgg ctctccgcag
    13621 ctctcgcagt agaacgcgag gatcggaact ccccacaccc gctggcgcga gatacaccag
    13681 tccggacgtg tgccgatcat ccccgaaatc cgttcctccc cccactccgg gagccaacgg
    13741 atctccctga ccttctcgat cgcacgggcg cgaaggtcgt ttctgtccat gccgatgaac
    13801 cactgctcgg ttgcgcggaa tatggtcggc ttgtggcagc gccagcaatg cggatagctg
    13861 tgctcgaccg tgccttcggc cagcaatgcg ttccgttctc gcaagatgcc gatgacaatc
    13921 ggattcgcgt cccagaccgt ctggccgatc aattcctccg gcagtcttcc cggtgcgccg
    13981 tcggcgtgga agaagcggcc gccggcgtcc accgggcagt aggtggcgat gccgtaccgc
    14041 tgccccacca cgtagtcctc gagaccgtgg ccggtggccg tgtgcacagc tcccgtgccc
    14101 tgctcgagcg tgacgtgatc cgcgaggatt ccgggcgaat cccgctcgac gaacgggtgc
    14161 cgaaagatcg caccttccag cttcgcgcct gtgaacgacg ccacgatctt cggatcgacc
    14221 caaccgcact tctcgctagt cgccttcagc aggtccctgg cgataaggta gacctcgccg
    14281 cgcacctcca ccgcgacgta ctcgaatttc ggatggtatg cgatggccat gttcgccggg
    14341 atcgtccacg gcgtggtggt ccagataact gcgtagacct ttcgcccggc gagcgccgga
    14401 tcgatcttcg ccgggtccga ggccaaccgg aagcgcaccc aaatcgacgg gctggagtgg
    14461 ttctcgtact cgacttccgc ctccgccagc gcggtccggc agcgcatgca ccagtggacc
    14521 ggtttcaggc ccttgtacac atagccctta tcgaggaact cgacaaaagc cccggcgatg
    14581 acggcctcgt acgaagcgct catggtcaga tacggatcgg cccagcgtcc gaagacgccc
    14641 agccgtttga actcgcgccg gtgcagttcg acgaacctcg cagcgtactt ccggcacgcc
    14701 gtgcggatct cggagggggt catcttggct ttccgggagc cgagttcggc atcgactttg
    14761 atctcgatgg gcagtccgtg gcagtcccag ccaggaatgt agggagcatc aaaaccggcc
    14821 gaactgtgtg acttgacgat gaagtccttc agaatcttat tgaaggcggt cccgagatgg
    14881 atgttgccgt tggcgtatgg gggtccgtcg tggagaacgt acatcgggcg gcccgcccgc
    14941 gcctgacgga cccgttcgta gatattcatc tcctcccacc gggccagcat cttgggctcg
    15001 gcgaggggca ggttcgcctt catggcgaaa tccgtgcgcg gcaggttaac cgtcttcttc
    15061 agatccacgt ctttcaattg catgtgaggt gtttatatta atatcgtaca caatgcccca
    15121 cttcccgctg ggtaattgtt gtcgatgtct tggattggcg ctgtttctcg cggcggttgc
    15181 gttcggagcg cctgagccgg ccggggtcaa ggtcgtcggt ctggacgcgc ggggtgggga
    15241 ggcggcaaca ggctccggct tctttgtcac cgccggcgga gaagcgattg tttcgcgccg
    15301 tgcgctgctc ggagcagtca ccgcggagat ccgcacggtg gaagggcggc gatatcaggt
    15361 gaaatcggtt gtcgcggact ccgtccacgc cgggttggcg cgcgtggccg tggatccccc
    15421 agtcaaagac tcgccgttca tcgccgtggt ggaggcggaa cccgttccgg gcgagaccgt
    15481 gacggttgca gggaccgaag cccgcatctc cgcggtccgc aacgtgcccg gtttcggtcc
    15541 tgtcttccgg atttccggca gtgtgcagcc cgaggacttt ggccgcctcg ttgtcaattc
    15601 gaggggcgcg tttatcggag tggttctctg gagcacgtcc gagccgagcg agatgaccct
    15661 ggcgggctcc gccgaaacag ttcactacat gaaatccggc tcccccaaga cactggcgga
    15721 atggaaccag ctcgcacgaa gcgcgccccc ggtagaagct gcttaccgtg acggtctcaa
    15781 ccggctgttc ctcgacgatc tcgatgcggc gctggagcga ttcgagtcgg tgctcgaaaa
    15841 agaccggcgg ttcgccgagg cttggtttca cgcgggcttc gtacaaggaa agctgggacg
    15901 ccggaaggag aagatcgatt cgtaccgcga ggcaatccgc ctgaagccgg aattcgccgc
    15961 ggtgcactac agcctgggag tatcctacgc cctgatgggc gagggccagt tagcggtgga
    16021 ggaattcaag gccctggaaa agttggattc cgagctcgcc gaaaagctcg agatcctgct
    16081 cgaggccctc ggccaccagg gccacggcca tggagagggc gagaaagtga agccgccccc
    16141 gaagcaggtc tgaatcaaaa ccgcctggga gcggctgccg tttcttactg tccgcccgcg
    16201 ccggcttcgc tcgttgcgtc gaggaacgcc aggtacgccg agagcgcttt gaaggtgttc
    16261 ttgtagtcgt ccatgctgac ggcggccaac gtgtctttct cgctgtgcgg gaccgacaac
    16321 tgctcctggc caaccgagtg gacgtcgatc gtcgggattt tcttgtccgc gaacgggtct
    16381 gagtccttcg tcgcgccttg cgccgcgttt gtgcccatga ttgggacctt taccgacgcc
    16441 gccacccggg cgaaccagtt caccaggttc ttgtcggact tgtcgataat caacttggtc
    16501 gccgccagtc ccacgcagtc gacgttcacc atcgccttcg cgccggctcg ctgctccgcc
    16561 gtcatctcct tcacgtacgc cttggagccg gcgagtcctt tctccttttc tgaaaacccg
    16621 acgaacacga aagtgacctt ccgcggatgc gcgctcaaac tctggtagag gctcggcagc
    16681 agggcggcgc cggcccagtt gtctatgatg ccgtcaccct tcttgccctt gtcgaagtgc
    16741 gcgccaacca cgatgctcgc aggactcgag cccggcagcg tgcaaagcac gttcggttgt
    16801 gccgcgccct tcaccgcctg ctcgctgagt tctttgcagc cggcctcttc gaacagcttc
    16861 ttcaaggtgt cgaatcgggc ctggttgtcg gccggcgcct tctggagtcg cgtttcaatc
    16921 acattcggag cgacgctatt gaattggatg gtctgcgcgg cgagaggtag cacggctgcg
    16981 gtcgtcacga caaggcagga aaacaggcga gtgcaagagc cgaaacggct gtagttcatg
    17041 aaatgactcc tttttttcgt tgacgggcga gagcgttggc gggatctttt ggcgagtcgc
    17101 cggcagtcgt acgtcacgga agcccgatca tgcccgaagc gcgcgtttgc ctgcttccag
    17161 cactttcagg atcgtctcca catcatatac acatccgccc caggcgccgc cactcacgtc
    17221 ctgaagcacc gcccacaaac gcgaatcggc cggcaggttc ggatccggtg aaaggccggg
    17281 gtgcggcggc cgctccgcga gcacccgggc gccttcctcg gcgtcgaatc gcctgccgtc
    17341 cgctccgacg aggttcaccg atccttcgag ccggttgcgg tcgatgacga tctctatcag
    17401 gtccccgtcc cgcaactttc caatcggtcc tccggcgagc ccctcgggcg atatgtggcc
    17461 gatgcaggcg cccgtggaga cgccgctgaa gcgggcgtcc gtcagcacgg cgacgtgctt
    17521 gccgaactcc agatacttca gcgccgatgt gatctggtac gtctcctcca tcccggatcc
    17581 catcgggccg cggccggtca gcacgaggac atcgccctcc ttgatacggt ccggtccgtt
    17641 gttcttgatc gccgccaccg cgtcgggttc gctggtgaat acacgcgcgg ggccggtctt
    17701 ccggtacaca ccgtccttgt cgaccacgct cgggtcaatg gcggtgctct tgatgaccga
    17761 accctccgga gcgaggttgc ccgccgggaa aactgcggta gaggtcaggc cgcgccggcg
    17821 ggccgtgccg ggattcatga ttacatcgtc tgggtccacg ccgtcgcggt ctttgagcag
    17881 cgcgcgcagg cggaaccgcc gctcggaatt ctcccaccag tccagcatcg tgccgagcgt
    17941 atctccggca acggtcattg cgttcaaatc cagcaggccc gcgcgccgca ggtgcagcat
    18001 cacctcgggc acgccgccgg ccaggaacac ctgaacggtc gggtagttct tggggccgtt
    18061 cggcaggaca tcgaccagtc tcggcacgcg gcggttgatc tccgcccaat cctggcggga
    18121 cggccgccgc aagcctgccg catgcgcgac ggcggtgagg tgcaaaacga ggttcgtgga
    18181 gcctccgaac gcggcgtgcg tcaccagcgc gttgcgtacc gaggcctccg tcaggatgtc
    18241 ccgcatgcgc cgcccccgct cctcaagcgc cagaagcgat cgggccgaac ggcgggccat
    18301 gtcgagccag atcggctggc ccgaaggcgc cagcgccgag tgcgggagcg acatgcccag
    18361 cgcttccgcc accacctgtg acgtcgccgc cgtacccagg aattggcagc ctccgcctgg
    18421 tgacccgcag gcgttgcatc cggccagcga ggcttcctcc aggctgatca tgccgtgcgc
    18481 gtagcgtgcg ccgaccgact gcactagcgc cgtatcctct ccgttctccg tggccagcat
    18541 cactccgccc ggcacgagca cgcaggggag gtcccgtgtc cccgccagcg ccatcatcat
    18601 tgccggcagg cccttgtcgc aggttgccac gccggccact ccccggcggg tcggcagcga
    18661 ccggatcagc ctccggaaca cggtggcggc gtcattccgg taagggaggc tgtcgaacat
    18721 cccgggcgtg ccctgagtgc ggccgtcaca cggatcggag caacagccgg cgaagggggt
    18781 ggcgccgtgc gacttcagtt ccttcgccgc cgcttcgacc agcaggttca cttcccagtg
    18841 gccggtatgg tagccgaggg cgaccggcgt gccgtccggt gcgcgcactc cgccgtgtgt
    18901 gctcagcagt aggaactcct tcgcgccgag cgaagccgga tcccagccca tgccggcgtt
    18961 ttgcgtccac ccgaacacgt cgccggacgg acgagcgagc agcatctccg gggtgagcgg
    19021 cagactgcca cgcggccctg gcgccttgct tgggacgtcg aaaatcgagt cgtcgcccga
    19081 atcgagaatc gacctgagtg acacggaagc catgacattc cgatgctagc agggccgcgc
    19141 gcccggaagt ttcagataac aataagaacg actggtcaag gctccggcaa cgcccccgct
    19201 tccgctagac tgagccaatg atagtcagaa tttggcatgg ctggacagcg ccgggcgacg
    19261 cggacgcgta cgaggcgctg ttgaaggacg aaatctttgt tggaatcggc gctcggcgga
    19321 tcgaaggcta ccgcggcatc gagttactgc gtcgggacag cgccgaagaa gtggagttca
    19381 tcaccatcat gcgcttcgat tcgatcgcct cggtccgcat gttcgccgga gccgattacg
    19441 aggtggccgt tgtccccacc gccgcgcggg ctctgctgtc gcggttcgac agccgttcgc
    19501 aacactacga actgagagag gcgcgcccga cgcactaaca cacacgctct cagccggcct
    19561 ccacgaggca tcatagctcg ccgctccgcg cagcccggca cccctgcccg cgccactgtt
    19621 acaataaaaa ttcacctcca caataaaggg cgatcaaatc agtggctgac aagcagcggg
    19681 ctgttgtggc aatctacccc gggtcgtttg acccgatcac gaacggccac ctggacctga
    19741 tcgccagagg ggcaaggctg ttcgacaggc tgatcgtggc ggtacttcag aacaccgaca
    19801 aggagccgct tttcagcgtg gaggaacgca cggagatgct gcgcgagacg atcagcctgt
    19861 acccgaacgt ggaagtggat tcgttcaacg ggttgctggt ggattacgcc gcgaggaaga
    19921 atgcatcggt gattttgaga ggcatccgcg cgatttcgga ctacgagtac gagttgcaga
    19981 tggcgctgat gaacacccgg ctttccccgg gcatcgaaac ggtgttcctg atgtcgagtg
    20041 aggcgtattc gtttatcagt tcgcggctgg tcaagcaggt gttcggtctg agggggaaca
    20101 tctccggact ggttccgccg gtcgtcgaac gaaggctgca aagccgggca atcgaaaaat
    20161 aactagaggg gcttgcgaat aggggcatgc tggtaacaac tgaacttgcg gagcgaatgt
    20221 cacgcatcag cgtctccgcc accatgaagg tggcgacgga cgccgagaag ctgcgggcgc
    20281 acgggatcga cgtggtggac ttcggtgcag gggagccgga cttcccgacg cccgacaaca
    20341 tcaagcaggc ggccgtccgc gcgctcgaag ccaacttcac gaagtacacg gcctccggcg
    20401 ggaccatgga gctcaaagag gccgtctgcg agcgccacgc tctcgacttc gggacctcct
    20461 acacgccgtc ggagtgcgtc gtctcggtcg gcggcaagca cgtgatcttc aacctgatgc
    20521 aggtgctgct caatccggga gacgaagtca tcatcccggc gccgtactgg gtcacgtaca
    20581 aggatgtcgt tcactacgcc gagggaaagt gtgtgttcgt cccggccgat gaggcgagcg
    20641 gcttcgaagt caccgcggcg atgatcgagg agcatctgac gccaaagacg aaagcggtcc
    20701 tcatcaactc gccctcgaat cccgcgggcg cggtgatcgg acgcgaagag tttgagcaca
    20761 tctaccggct cacatccaaa cgcggagtgt ggctgatcac cgatgaatgc tactgccatt
    20821 tcctgtacga cagcgctcca ttctcggtcg catctattcc gggcgcgcgg cactcggtga
    20881 tcgttgccag ctcgctttcg aagacttacg ccatgaccgg ctggcggatc ggttttgctc
    20941 tagcgccgcc gccggtggtg gccgccatcg ccaaactcca gagccagtcc acgtcgaacc
    21001 caaactcgat cgcgcagaaa gccgccgtcg aggccctgcg tggtccccag gactcggtgg
    21061 acgcgatgct ggccgagtac cgccaccgcc gcgattttgt gatccggcgt ctgcgacaga
    21121 ttccgggcgt cacgtgcgcc gaaccgaagg gcgcattcta tgcgtatccg aacatcggcg
    21181 cggccatcgg caggaacggc attcaagata cgctgagttt cgccgagcag gttctcgacg
    21241 aggcgcgcgt cgcggttgtg cccggagagg ccttcgggac tgccgagcac gtgcgaatct
    21301 cttacgcgac gtcgatgcac gaactggagc gcgggctgga tcggctgcac aacttcatca
    21361 cgaacctcgg ctgatggacc tcggaccgtt tcgcctcatt ccgtcattcc gggaaaaagt
    21421 ctggggtgtg acggatctcg ctccgtggtt tcctcaagct tctggaaaga tcggcgaagt
    21481 ctggtttctc cggccagacg acgttcccca gccgattctc gtgaaaatca tcttcacttc
    21541 ggcgctcctg tcggtgcagg tgcatccgca tgacgcctac gcgctcgcgc acgatggaat
    21601 tccgggaaag accgagatgt ggtacatcct ccgcgcggag cctggggcaa agctggcgct
    21661 cggcctgcgc gagcctgtca cgcgcgaggc gctgaaagag gctgccgcgt ccggcgtggt
    21721 ggggcaactg cttcagtggt tccccgtgag tcccggacag gtcgttttca tcccgccggg
    21781 aaccgtgcat gcaatcggtc cgggaatcac gctctgcgaa atccagcaga attccgacat
    21841 cacctaccgg ctgtacgact acgggcggcc cagggaactg cacgtcgaca aggcgttgga
    21901 tgttgcgact ctagggcctc accccggtcc gtccgccccc gctggcgcca cgctcgccgc
    21961 gtgcgactac ttcatcaccg aacagatcga gatcggccgc gctaccgcct atactccgga
    22021 tcgtgatcgc tttcacctgc ttgtgtttct ggagggcgca ggcacaatcg ccggacagcc
    22081 gtttcgcgcg ggagaagtct ggcatgtacc cgagggcgcg ggaacgtttt ccctcgaccc
    22141 ggacgggccg gtgcgcctcc tccgatcctc tccgccgcac cgttgataaa ctggtacacg
    22201 gtgctgaaaa acccgcgcca caggctgaag cctgtgccac cgcaggccat aagcagttga
    22261 ttctgagtgg tggcacagcc tttaggctgt gtcgggcgcc aacccaggcg ggttttcagc
    22321 accctgtgga agtcttcgcc gatcaccgat gaaccggagc cgtgtccttt gcatccttct
    22381 tccgcttttc agtctgctcg ccggctgcgg ctcgcgacgg agcggtccgc cggccatcgg
    22441 cgaagcgtac gtcggcccgg cgaccctcaa tatccgcaaa gatctcggac tacaccggcc
    22501 aaccgtggcc acggtcaagc acggcgagcg tctcgatatc gtcggccggc gccgggcgtt
    22561 cgtccaggtg cggacggaga gtggcgccat cggctgggtg gacggccgcc agctcttcac
    22621 cactgaacag atggccgagc tgcgtagcgt ggccgatcgg gccgcggcgc tgccctctca
    22681 aggccaagct tccattcagg aagcgctcaa tgtccacacc catcccaatc gccagtcacc
    22741 cagcttccgg cagttgaaga ccggagagcg ggtggacgtc gtcgctcacc gtctcgagcc
    22801 gcgcgtgccc ttccgtccgg accgcccggt acaatcgttg cctccatcgg ctccgatgga
    22861 ggactggagc ctggtccgca ctgcggacgg caaagccggc tgggtactct tccggttgct
    22921 cttcatggcg atcccggacg aggtggcaca gtatgcggaa gggcaccgca tcacctccta
    22981 tttctcgctc ggagaagtcc atgacggaga cgccgtcaag cacaactggc tctggacaac
    23041 gatcgcccgt ggcctggagc cgtacgagtt cgacggcttc cgtgtcttcg tttggagcct
    23101 aagacgccac cgctacgaaa cggcgtatat cgagaggaat cttcgcggct actacccggt
    23161 ggaactccat cccgagcagg gcggcgcacc gagtttctct ctgatcatcg aggataaaga
    23221 cggggtgcgc tccaaacgga cctatgcgtt tcagggatat cgcgtccgcc tgattgcaac
    23281 tgagccctgg aaccccggga gcgacgctcc ggagcgcatc gccggcgccg agccgccttt
    23341 ccaggagcag gatcaacggt ctttcttcgg ccgcctgtgg gacaaaataa aggggatctt
    23401 cggccgctga ctctcagtcc tccgcgccgc ttacggcggc tggaggtgcg ccggcagcga
    23461 gttccactgc tttcttcgca cggcttagat cgttggcggc gagtttgatc cgcgccgact
    23521 gagtccccgc caccgacagc cacgcgctcg tgccggcgcg ggcgcgacag ccgtgtacaa
    23581 aggcatcccg gacgttttgt agccggaccg cgcatggagc gtccggcaaa accgccaggt
    23641 ccgttcccgc aagctccaga ccttcaatat cgtcacagat catcgccgcc cggccgtccg
    23701 gtttctcgaa gccggcctcc acgttccgga acgcgacgtt gctcgcgtgg cggcagtaga
    23761 acccgtaggc cggcagtttc ccgaacatcg cgaattccgg gtacttttcg ggaacttcgg
    23821 gcacctcctt gccggggtct tctttttcgc ctccaccggc aaacgagatc tgcacgttgc
    23881 tcagagtgac gttctcgatc gggtgttccg gaattccgga aatcgcgcag ccgaccgaac
    23941 ctccgccggt gatctgcacg tcgctgatct gcacgttccg gaggcgcccg atgccgggcc
    24001 gctgtccgcc ctcgacgaac ggacgggcgc ggttcccgag acggataaag atggggccgc
    24061 ctacgttccg catggtgatg ttcgagacgt tcaccttgtc gagcacgcca ccgtccacgc
    24121 actcgatcgc aataccgccg agccgtgtgt cgtagatcgt cgagttggag aagacaatgt
    24181 tttcgaagcc gccgttggtc tcggtgccga gcttgaaggc gttacaggcg gtgctcagca
    24241 cacagttggt gacggcgacg tccttgcacg gtcggtccgc ggtgctcttc agcacgatcg
    24301 cgtcgtcgcc cgagctgatg tcgcagtccg agatccgcac acgctcgcag caatcgatgt
    24361 cgatcccgtc gttgttgtga ttcacgcggc tgtggactgt gatgccgcgg atggccacgc
    24421 catcgcaggc gaggtagtgc tgcacccaca tcggcgagtc cttcatcgtg acgccggtga
    24481 cggacacgtt ccggcactgg atgatccgga tcatgtacgg ccgcaccttg tatggaccct
    24541 ggaatgcggc gccctgcccg tcgatgacac cgcggccctc gatggcgatg tcttcgacgt
    24601 tctccccgta aatgaggctc ttgtcggtgt aattgtcggt gtaggaccgg aacgcgggca
    24661 cggttatcgg atagtgctcc agcttgcggc tgccgagcag cacggcgccg gcgtcgaggt
    24721 acaaggtgac gcggctcttg aggaaaatcg ttccgctgag atac
//
LOCUS       DATIFQ010000293        17075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1244728, whole genome shotgun sequence.
ACCESSION   DATIFQ010000293 DATIFQ010000000
VERSION     DATIFQ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17075
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244728"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..635
                     /locus_tag="VLE22_13820"
     CDS             240..635
                     /locus_tag="VLE22_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSB15530.1"
                     /translation="MTEAQRTSRKTALEAKRRELSGEIRNQAAELAISDDVHDPVDQV
                     QNTIYRDEAGEHAHRLFRTLLSVEDSLRAISEGSYGYCAECGELMAAKRLDVIPWATH
                     CVPCQTQRESTLESQAVPIPTFGEERKAA"
     gene            complement(1418..3058)
                     /locus_tag="VLE22_13825"
     CDS             complement(1418..3058)
                     /locus_tag="VLE22_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB15531.1"
                     /translation="MRTLTNRVGFAACVLGLLIASAAAQTAEPQRLRVTTRLVEVNVL
                     AESKNGDPAVGLTREDFLLFDEGRPQEIEFFSAEKLKPPAPPKPLPPNIFTNRLEYLA
                     GPVNSATVILFDGLNTPLTDQAYARQQIIKFLKEITPEDRVALYVMGRGPRVLQDFTS
                     DSAALLKGLADYKAAPVASLEAPLYDPGVSGADHFDTWLGELSFNLYSHFARDRAYRT
                     VRALIAIAQHLERLPGRKNLIWVSGSFPIWIGDNSVPLPRKAAGRDPVWPEIERAARA
                     FNRSNLAIYPVDARGLMAPQEYRADRAEAVRDSLAADQAIFSTMRTLAERTGGRAFYQ
                     NNDLRAAMRKAADDARLTYTLAYRPSHGRWDGKFREIKIQVKREGTELRYRRGYFAQP
                     DEPGESWYRKGILDAAMWSPMEATRLGLTVRVSSAGANSLSLAVQVDPNELACRPDGN
                     VWECGMDIWLVQLDGRELHINTVARTSTMRLEQPTYLKAMEARGLVFMEKLDASPKAK
                     LVRLLLRDVSSGALGSVTIPIQRFLATAGKDAGSSTGR"
     gene            complement(3092..4192)
                     /locus_tag="VLE22_13830"
     CDS             complement(3092..4192)
                     /locus_tag="VLE22_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15532.1"
                     /translation="MKLAVCVALVLIPAAVFGAGDLNYDGMAARIAVALKLRPGERVL
                     LRYDPGYFAEILPPLRKQIRAAKAEFLRPLLAKDPVPVGKLSAADVFIRLPLGSSARA
                     LTAAEETALVKWVDGGGRRRELHFHWSDGSRLRDGLPGEHPAEYDALYQDALDIDYEA
                     LRAVQDRAIRTLRLGTVRVSTPAGTDLMFRIAKRPFNKQDGDASGERAQAARVRVDRH
                     IELPAGVIRVAPVEATVCGQFVIPEARFGDKIARKVVIQIDNGRVRSIKAAEGQEALN
                     AELAKGGEAAMRFREFALGLNPKLIASPDKAVVPYYGYGAGVVRFSLGDNEELGGDVR
                     GSFVRWFFLTDATVHVDYRYLVKGGKLVMEGL"
     gene            complement(4211..5368)
                     /locus_tag="VLE22_13835"
     CDS             complement(4211..5368)
                     /locus_tag="VLE22_13835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB15533.1"
                     /translation="MERRSSRRSFLGAGLALPAARLASAQSKDAVKLEHRTLGKTGLK
                     VTSLGFGCMLVPDPIVIERAYDLGINYFDTARMYQNGNNERMVGAALKGKRDKVVLTS
                     KTRTFTKQDALSDLETSLKEIGTDYLDIWFLHIKNEPADVTEELLEAQRIAKQAGKIR
                     FAGVSFHFNMPTMLAHVAKLGQTDVALASYNFTMEPAVGEAIRAARKTGMGVVAMKVM
                     AGGYARILKGDRLYGQKPSELLSRLKQPGAMLSALKWVLRNESVDTAIIGITDLDELD
                     EDIRAMSERFGKDDEKVLAAYLDRIRPLYCRMCGECGGVCDRGVRVADSLRFLTYADG
                     YGQFAMARNEFLQLPKGSRPDVCGSCDACTVQCPNGVDVRARLSRAQELFA"
     gene            5476..6090
                     /locus_tag="VLE22_13840"
     CDS             5476..6090
                     /locus_tag="VLE22_13840"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB15534.1"
                     /translation="MALLVSFAASAPAQWEVGVAVGYGIYRNASVYAPAGKVAAGFRN
                     RFTVGAVIGEDLYNRVGGEFRYTYQDGDPFVAAAGVKTNVQGQSHAFHYDFLFHVRER
                     GARIRPYAIAGVGAKLFVVSGPASPVQPLENIVRLTTESDFKPLVTGGGGVRMMLGKH
                     VVLRLDFRDYITPFPKKVIAPAPLGTARGIYHMFTPMAGLSWIY"
     gene            6179..6649
                     /locus_tag="VLE22_13845"
     CDS             6179..6649
                     /locus_tag="VLE22_13845"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB15535.1"
                     /translation="MPLTFRLARPEEYNALESFTIDAFGPITWLRTADVTFGPLNGLD
                     WRQRWQRRFRKVFDEQIVLVGETEGEVVALATGTIDRDALLGYIDLLAVHSNHHRKGY
                     GREMLRGMLAHMKSEGAVHAHLECLATNAAGNALYASEGWTEVARSLRWFIKIP"
     gene            6646..7560
                     /locus_tag="VLE22_13850"
     CDS             6646..7560
                     /locus_tag="VLE22_13850"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSB15536.1"
                     /translation="MKRRTFLANTMAAAVVQPTPGEVFIERPVSGKPHAGKVLAAIQP
                     HSDDVPIFSAGLVLKLIDEGYTGYLIRVTNDDMAGPGTVGDTVLANERDNREVSRLLG
                     LKQVFDLNYSNHQMDQESPLEIRARFIFLFRLLKVDTIISYDPSGHYEENPDHYVTAQ
                     AVEAACWMAGMNKDYPEHFAAGLQPHGVRERYYYARGPQLVNRVIDISGVIDRKVEVN
                     LANKAQGPAGENGARLRARLASQNLRLPILGDSDDTANRQYTKHFVLARDAEWGKKYG
                     LEYAEPYHYIGPEPPVLEDYIKKNAMPL"
     gene            7667..8236
                     /locus_tag="VLE22_13855"
     CDS             7667..8236
                     /locus_tag="VLE22_13855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSB15537.1"
                     /translation="MNTRTTVMEPSNEQLYCRTRKGDQGAMETLYERCEPPLYRYAFQ
                     LSGSRAVAEEVTHEAFLQIIRPESRFNPDRGSLEAYLYGTVRNLIRAARRSQAVEGIE
                     EPAAEDDILRALIDEEMSLALHSALQELPSPFRDAVFLCDLEERSYEEAATTLGCPVG
                     TIRSRLHRARQLLAARLKPLRLSAVSIAR"
     gene            8242..8754
                     /locus_tag="VLE22_13860"
     CDS             8242..8754
                     /locus_tag="VLE22_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15538.1"
                     /translation="MDRKDWLEDEEVLQEPIRRLRAMLAEERSPEWRRQRLMAEFVRL
                     HRRSGGWLAWATRAAAAALLALAVTLVWRGAPESAQRTGIEVAQPGIWNHVLDGSMDE
                     NGFAPIPYAMPLAAGECIRVMRTEVTGAALAQMGMSLPWGYEDDFDVDILFGDDGFPR
                     AVRMVSNTEI"
     gene            8779..9603
                     /locus_tag="VLE22_13865"
     CDS             8779..9603
                     /locus_tag="VLE22_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15539.1"
                     /translation="MNKTGLAIFATLTAAAILSAQATPPGGPVRGPFGVVGGVPGGGA
                     GGSIVVGPKPDMLIPGAPFSGVQVREVRQSLPNGNQIQRLEDTKFYRDGQGRMRIEVP
                     QPNPTNDRAPVLIEIFDPVAGAAYILDPSNLTGRKSAFLLPKTQGRSTRKRQAGQVQE
                     EDLGIRKVNGFEATGRRATQTIPAGAIGNRQTILSVTETWTSTVLKIPILHKRSDPRF
                     GDSTVQLTQIALGEPDPSLFQVPENYKLTPGGPFGPGIVGGVKAATKSPARERSKD"
     gene            9810..9959
                     /locus_tag="VLE22_13870"
     CDS             9810..9959
                     /locus_tag="VLE22_13870"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HSB15540.1"
                     /translation="MLWLIFWILLAMWLLGMVTSYTLGGFLHILLILAVVAVLIRVIQ
                     GRNPV"
     gene            10050..10511
                     /locus_tag="VLE22_13875"
     CDS             10050..10511
                     /locus_tag="VLE22_13875"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSB15541.1"
                     /translation="MATQPATKPVEQDLLDLEKQYWQAVKDKDVDAAMHLTDDQCLVT
                     GAQGVARIDRETLASMLKATPYTLNDFEVSDDAHVRLLRDDVAIVAYKVREELTVDGK
                     SLTLEAADASAWVRRDGRWVCALHTESISGDPFGRNGRRKSRRSSRHVRRS"
     gene            complement(10613..14074)
                     /locus_tag="VLE22_13880"
     CDS             complement(10613..14074)
                     /locus_tag="VLE22_13880"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15542.1"
                     /translation="MKFSSFRFAPVWLVVILCVAVGVASAQEYRARVQGFVTDPSQAA
                     VVGAKVTLRNMNTGIETSRETDAMGRYLFDFVQPGTYAISVEMSGFNRYLQENIAVLT
                     RGDVTVNAQLVIGGVAETVTVTANVATVQFNTSTMTTTVQGNMLKDIPILAWNPFTLA
                     LLNPAVINRYWDVAHRNPFYMWSSNGLDVGGRTGGKNDMLLDGVPLGVSARGSYMPSM
                     DAVQEVAVQQNALDAEFGFSAGGTLNVSMKSGTNEFHGTGYYFGRNPTLNAMTNRVTG
                     GFSVVKNHIWGGTVGGPIVKNKLFFYQTYEQWKSTQPSSKELTMPTDLQRTGDFSQTF
                     QKDGSLRMIYDPFSTVFDPATGTATRTPFTNNKIPSDRINPTSAKITSDLWKPNGPGI
                     DASGMNNYLISYAWWLKYWNISNRVDYNINDKWRMYARFSKYETRLDNPNWGGTIAVP
                     SDNGGLMDSLNPAIDFLWMVSPSTTVNMRYGATYLEDDYNSAWSQVPESVWAGLYPNG
                     WYKPMLEGLPGIYYPRYNFSGNGSAYTGFGSWWLVRGRSHNSNLNVTHDRGIHHMKAG
                     WSLRYQYDQNGNPGPGSFTFNSIDTGKSFLGYSAATSGDMYASMLLGVANTGNAQITP
                     MVDSHQQMWGFFFQDDIKLTRRVTLNLGLRYELETAPSEYSRLYSRYLDLTNPIPELS
                     GGVTMPAEVTAIASIPYKWNGAWVFTDNDHPGVFNSQKNVFLPRIGAAIRINDNTALR
                     VGFARYAVPWVTIYPETNSWPKDGFARSTPIISPIEGKPQTIISDPFPVSNPLQTPIG
                     KSLGRYTNLGANATFFPQDMIHPINDRFNVSLQRQAPFRILTDTTFFMNLGRNVQDAS
                     MWGGDYNYNLNMVDPNYGYTYKGTTTKAVANPFYMLLPADKMPGSLRTQKTVSVNSLL
                     APYPQYGTLTERFLRDKDNHYYSLQFKAERSMAQGLTFSFGYNYSHETRDEFFDSIAT
                     YNKNFTMIDTRDPRHYVRLAGTYELPFGKGRQYLSGANRILDALVGGWATSHIFMWNG
                     GPQLTFGAMNCTGDPKIDNPTRGQWFDTSKCSVLPAYTPRTNPWYYDGLRGPGFWQWD
                     ATAVKYFQITERVKFELRMEFYNFPNSFMPSQPNMSVTSSAFGKSTSIAGGNYGREVQ
                     YTGRIHF"
     gene            14260..15186
                     /locus_tag="VLE22_13885"
     CDS             14260..15186
                     /locus_tag="VLE22_13885"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HSB15543.1"
                     /translation="MGRAVVLFRRFAWGVLGYTLLVVLWGAYVRASGSGAGCGSHWPL
                     CNGEVIPRAQQVETLIEFTHRLTSGLSLLAVAALYVWAFRLFPRGSRVRRFAVLSVVF
                     LLIEAALGAGLVLLRYVAQDASSGRAIYLSAHLVNTQILLAMLALTAWFATAQPSRNW
                     LSDPISLTALAIALAVSVTGAIAALGDTLFPATSLAAGLRDELSGDSHALLRLRLLHP
                     AAAIAGAAFLLWMALRAIRSDIGPAAKTAARWTALLVFLQLIAGAANVALLAPIWLQL
                     THLLIADLLWLALVLLAVSALQPADAKSAVTR"
     gene            15253..16938
                     /locus_tag="VLE22_13890"
     CDS             15253..16938
                     /locus_tag="VLE22_13890"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HSB15544.1"
                     /translation="MHTSRVLIAILTVVALGAAGCGAGSSLRTWYVDSLTKVFPDDKP
                     GTNERSGKTWLVARNGHVSVQIALRADKAIPQLRAELKAPENSGQKLTAEARWVEYVP
                     VGSNPPRTPYDEVVRPAPSLFPDPLMEKWPFDLKAAATQPVWITVYAPADAPPGEYDG
                     EVTFFAGQNRLKSVDFAVHVTAATVPAEQKLKVTNWFTFTGERLHNHYEVEDYSDKYW
                     EILYNFGRVMADHKQNVIITPIMPLTKPSMAGGTMRFDFSLLDKWVETFQKAGVIGTI
                     EGRHLLGRGGGYYSPVVVQAYVVEGGEIVQKGLDPDDPRAEQFLNTYLPALYAHLKEK
                     GLTDHYLQHVLDEPHDREAAIYNRYAKIVRKHLPGVPTIDAVGLEQDISFFEDVCDIW
                     VPVLSSFDHQLDKMRAHVEKGGKMWYYTCVSPQGRYLNRFTDLPLVKTQLLHWFNFRY
                     DLTGFLHWGGNAWGPRPFMNVQTVINRNRTLLPAGDNAIVYPNAEKLSVLSSIRLEAM
                     REGIEDYELLVALADKDQAKAKGLAKTAIPNINDYIRDVSAFRNVKRQLVEAF"
BASE COUNT         3154 a         5379 c         5219 g         3323 t
ORIGIN      
        1 gccatcagtt ggccgatcca actgaacggt aagtgcggat tgaagctgat tgcccatggg
       61 cggatcgtgc gctgccaggg cacaaaggtc gcgctggaga tcgagagaca cgaattccgg
      121 actcgtctgg gcaagcgggc agctggatcc tctttgctcc ttacgcccgc tctccccgct
      181 gcaagagggg caatcgtgtc tttcacggaa ctatcggcgg gaacaagggg ccattacaca
      241 tgacggaagc tcagcggacg agccgcaaga cagcgcttga agcaaagcgc agggagctgt
      301 ccggcgagat tcgcaaccag gcggccgaac tggctatcag cgatgacgtg catgaccccg
      361 tcgatcaggt ccagaatacg atctatcgcg acgaagctgg ggagcacgcg caccgtctct
      421 tcagaacgtt gttgagtgta gaggattcgc ttcgtgcgat atccgaaggc tcctatggct
      481 attgcgcgga gtgcggggag ctgatggcgg caaaacggct agacgtcatt ccttgggcaa
      541 ctcactgcgt tccatgtcag acgcagcggg aatccacgct ggaatcccag gctgtaccta
      601 tcccaacttt tggcgaagag cgtaaggccg cataacgggc ctgcggtatt ccccagataa
      661 tttcaaatca tttgtccctt tttttgtctg caggtgacgg gcaaacctgg gaattttacg
      721 agttccacat cagggagcta tacgccacaa tcagggtagc ggtctagtta tccggagcgg
      781 gcaaccgtgg ttgacaatgc tttcttttgg tttggggctt tcagggcgag cgttctcgtc
      841 ttaggcggct ctgggttcta acgtcgcagc gctcgccctg agaagtgaga tgaacgagga
      901 tcgttccgag gtggcggctc ggagggcgcc gcctcgactt ttttgaaggt cgccctgggg
      961 gtcggagggt gcgtggcgca cctgggcggc ggaggctggc ggtgtcgaac ctcatcacga
     1021 gtgccgattg cccgagatgc aagcggggca ccgcggtgga atcctgtacg ggttactccc
     1081 tgaacatcgc aggagcggag tacttccgcg ttagattcgg aagcgaacgg ggacgcttac
     1141 acttgattgg cggccggtgt agggattgcg gcgtcaccca cggaagcctc caccatctgc
     1201 actgtgaagt ggaggagtgt ccgaaatgcg gcggccatct cctcgcgtgc gactgtgtct
     1261 ctctcacaag ccccgtgtag cagttcactg gtttccgaga tcgctgggcg agaaacccca
     1321 tcgagacgcc gttacgtttg ctgttcgtcc tcttcctgac taccgcagcc cctgccgagg
     1381 aacagccgga gtagttcctg gctgtcgtta gacagtccta acgtccggtg ctcgaaccag
     1441 cgtctttccc tgccgtagcc aggaagcgtt ggatcgggat ggtcaccgaa ccgagcgcgc
     1501 cggaggagac gtcgcgcaga agcaaccgga caagcttcgc cttcggcgag gcgtccagtt
     1561 tctccatgaa gacaagcccg cgggcctcca tcgccttcag gtacgtgggc tgttcgaggc
     1621 gcatcgtgct ggtgcgggcg accgtgttga tgtgaagctc cctgccgtcc agttgcacca
     1681 gccagatgtc catgccgcac tcccagacat ttccgtccgg ccggcaagcc agttcgttcg
     1741 ggtcgacctg caccgcaagg cttagggagt tcgcccccgc cgagctcaca cgcactgtga
     1801 gtccgagccg cgtcgcctcc atcggactcc acatcgctgc atccagaatt cccttgcggt
     1861 accacgactc gcccggctcg tccggctgcg cgaagtatcc gcggcggtac cgtagctcgg
     1921 tcccctccct cttcacctgg atcttgatct cgcggaactt gccgtcccag cgtccgtgcg
     1981 agggccgata ggcgagcgtg taggtgagcc gcgcgtcatc ggccgccttt cgcatggcgg
     2041 cgcgcagatc gttgttctga tagaacgccc ggccgccggt ccgctccgcc agcgtccgca
     2101 tagtactgaa gatggcctga tccgctgcga gagaatcgcg gactgcttcg gcgcggtccg
     2161 cgcggtattc ctggggcgcc atcaggccgc gggcatccac cggatagata gcgaggttcg
     2221 agcggttgaa ggcccgggcg gcacgctcga tctccggcca caccgggtcc cgtcctgcgg
     2281 ctttccgcgg caagggcacc gaattgtcgc cgatccagat agggaagctg cccgacaccc
     2341 agatcagatt cttgcggccc ggcaaccgct ccaggtgctg cgcgatggcg atcagcgccc
     2401 gtaccgtgcg gtatgcgcgg tcccgggcga agtggctgta caggttgaag ctcagctccc
     2461 cgagccaggt gtcgaagtgg tccgcgccac tgacacccgg atcgtatagc ggcgcttcca
     2521 gagacgccac tggagctgcc ttgtaatccg caagaccctt gagcagcgcc gccgaatcgc
     2581 tggtgaaatc ctggagcaca cgcggcccac ggcccatcac gtacagcgcg acacgatcct
     2641 ccggcgtgat ctccttcaag aacttgatga tttgctgccg ggcgtacgcc tggtccgtga
     2701 ggggcgtatt cagaccgtcg aacaggatca ccgtggcgct gtttaccggc ccggcgagat
     2761 actcgagccg gttcgtgaag atgttcggag gcagaggctt cggaggtgcc ggcggcttca
     2821 gcttttccgc gctgaagaac tcgatctcct gcggccgccc ttcatcgaaa agcaggaaat
     2881 cttcgcgcgt gagtcccact gccggatcgc cgtttttcga ctcggccagc acgttcactt
     2941 cgacaagccg cgtggtaacc cggagccgct gtggctccgc cgtttgcgcc gccgccgagg
     3001 cgatgagcag cccaagcacg cacgccgcaa accccactcg gttggtcagt gttcgcatga
     3061 agcttccgag ccgcgaccgc agggagcggc gctacagccc ctccattacc agtttgccgc
     3121 ctttcaccag gtaccggtaa tccacatgga ccgttgcgtc agttagaaag aaccaccgta
     3181 cgaagctccc tcggacatcg ccgcctaatt cctcgttgtc gccgaggctg aagcggacca
     3241 cgccggctcc gtatccgtag tacggaacga ccgctttgtc cggcgaagcg atcagcttcg
     3301 gattcaatcc gagagcgaat tcccggaagc gcatcgccgc ctcaccaccc ttcgctagtt
     3361 cggcattcag ggcttcctgc ccttcagccg ccttgatgct ccgcacacgg ccgttgtcga
     3421 tctgaatgac gaccttccgc gcgatcttgt ctccgaaccg cgcttctgga atcacgaact
     3481 ggccgcacac ggtggcctct acgggagcca cgcggattac gcccgccggc agttcgatgt
     3541 gccggtccac gcgtacgcgg gccgcctgcg cgcgctcgcc cgacgcgtcg ccgtcctgct
     3601 tgttgaaggg acgtttggcg atgcggaaca taaggtcggt tccagcgggc gtcgagaccc
     3661 gaacagtgcc gagccgcaac gtgcgaatcg cccggtcttg cacggcgcgg agcgcttcgt
     3721 aatcaatgtc gagcgcgtcc tggtaaagcg cgtcgtactc cgccgggtgt tcgcccggca
     3781 gcccgtcccg tagccggctg ccgtcggacc agtggaagtg aagctcgcgg cgcctgccgc
     3841 cgccgtccac ccacttcacc agggcggtct cttcggctgc ggtgagcgca cgcgccgagg
     3901 accccagagg aagccggatg aacacgtcgg ccgcggagag cttccccacg ggaaccggat
     3961 ccttggcaag cagtggacga agaaattccg ccttcgccgc gcgaatctgc tttcggaggg
     4021 gcggcaggat ttccgcgaaa tagccggggt cgtagcgcag caatacccgc tcacccgggc
     4081 gtagttttag cgcgacggcg atccgggccg ccatgccgtc gtagttgagg tcgccggctc
     4141 caaacaccgc ggccggaatc agcacaagcg cgacgcagac ggcaagcttc atgcgtcctc
     4201 ccccgggcgc ctacgcgaac aactcctgtg ccctgcttag ccgcgcgcgg acatcgacgc
     4261 cgttcgggca ttgcactgta catgcgtcac agcttccaca gacatccggt cgcgaaccct
     4321 tcgggagttg gagaaactcg ttccgcgcca tggcgaactg accgtagccg tcggcgtagg
     4381 tgaggaaccg cagcgagtct gccacacgaa ctccgcggtc gcagacgccg ccgcactcgc
     4441 cgcacatccg gcagtaaagc ggccggatgc ggtcgaggta cgccgccagc accttctcgt
     4501 cgtccttacc gaaccgctcg gacatcgccc ggatgtcctc gtcaagttcg tcgaggtcgg
     4561 tgatgccgat aatcgccgtg tccaccgact cgttccgcaa cacccacttg agcgcggaca
     4621 gcatcgctcc cggctgcttc aatctgctga gcagttcact cggcttctgt ccgtacaggc
     4681 gatcgccttt cagaattcga gcgtagccgc ctgccatcac cttcatggcg acaacgccca
     4741 tgcccgtctt ccgcgccgcc cggatggcct ccccgaccgc cggctccatg gtgaagttgt
     4801 acgacgcgag cgcgacgtca gtctggccca gtttcgccac gtgcgcgagc atcgtaggca
     4861 tattgaagtg gaagctcacg cccgcgaagc ggatcttacc cgcctgtttc gcgatgcgct
     4921 gcgcctcgag caattcctcg gtaacgtccg ccggttcgtt tttgatgtgc aggaaccaga
     4981 tgtcgagata gtcggtcccg atctccttga ggctcgtctc cagatcgctg agcgcgtcct
     5041 gtttggtgaa agtgcgggtt ttgctcgtga ggacgacttt gtctcgcttc cccttgaggg
     5101 ccgctccgac catgcgctcg ttgttgccgt tctggtacat gcgggcggtg tcaaagtagt
     5161 tgatcccgag gtcgtaggcg cgctcgatga cgatcggatc ggggaccagc atgcacccga
     5221 agccgaggct ggtgaccttc agcccggtct ttccgagagt acggtgctcg agcttgacgg
     5281 cgtccttcga ttgcgcggag gccagcctgg cggcgggcag cgcaagcccg gccccgagga
     5341 agctgcggcg ggaggatcga cgttccatat cgccttccat gatacagaat cggaccggat
     5401 tatcttgcgc cccgccggac ccgcttgccg cccgtccctg gccgttgtct acttggcagt
     5461 gtgagcctgc ggatcatggc cctgctcgtg tctttcgccg cttccgctcc cgcacagtgg
     5521 gaggtcggcg tcgccgtggg ctacggcatc tatcgcaacg ccagcgtcta tgcccccgct
     5581 ggtaaagtgg cggctgggtt ccgcaaccgg ttcaccgtcg gcgccgtgat cggcgaggac
     5641 ctttacaatc gcgtcggagg tgagttccgt tacacgtacc aggacggcga cccgtttgtc
     5701 gcggccgccg gagtgaagac caacgtccag ggacagtccc acgctttcca ctacgatttc
     5761 ctcttccatg tccgcgaacg cggtgcacgg attcgcccct acgccattgc aggtgtcggc
     5821 gccaagttgt tcgtcgtatc gggtcccgca agccctgtcc aaccactgga gaacattgtc
     5881 cggctgacga cggagagcga cttcaaaccc ctggtcaccg ggggcggtgg tgtccgcatg
     5941 atgctcggca agcacgtcgt cctgcggctc gacttccgtg actacatcac gccgtttcca
     6001 aagaaagtga tcgcgcctgc gcccctcggc accgcacgcg gaatctacca catgttcacc
     6061 ccgatggccg gcctgagctg gatttattga aggcgattga aggcggatag tctccgcagt
     6121 gaatccggcc cgcttgaacc acaccgcgcc ccgcgcatac aatcggcgct ggaaaaccat
     6181 gccgctgaca ttccgtctcg cccgtcccga ggagtacaac gcactggaga gtttcacgat
     6241 cgacgccttc ggcccgatca cctggctgcg gaccgccgac gtaaccttcg gtccgctaaa
     6301 cggcctcgac tggcggcagc gctggcagcg gcgcttccgg aaagtcttcg acgagcagat
     6361 cgtcctcgta ggtgagacag agggcgaagt ggtggctctt gccacaggca cgatcgaccg
     6421 cgacgccctt ctgggctaca tcgacctcct cgcggtgcat tctaatcatc accggaaagg
     6481 gtatggccgc gagatgctcc gcggcatgct ggcgcacatg aagtccgaag gagctgtcca
     6541 cgcgcacctg gagtgcctcg ccacgaatgc cgccggcaat gcgctctacg cttcggaggg
     6601 ctggaccgag gtcgcccgct cccttcggtg gttcatcaaa atcccatgaa acgacgcaca
     6661 ttcctcgcaa acacgatggc cgccgcggtg gtgcagccca cccccggcga agtcttcatc
     6721 gagcggcccg tctccggcaa gccccatgcc ggcaaggtgt tggctgctat ccagccacac
     6781 tccgacgacg ttccgatctt ctcggccggc ctcgtcctga agttgatcga cgaaggctac
     6841 accggctacc tcatccgcgt caccaacgac gatatggccg gtcccggcac cgtcggcgac
     6901 accgtcctcg ccaacgaacg cgacaaccgc gaagtctccc gcctcctcgg cctcaagcag
     6961 gtcttcgacc tcaactacag caaccatcag atggaccagg agtcgccgct cgagatccga
     7021 gcccgcttca tttttctgtt ccgcttgctc aaggtcgaca caatcatctc ctacgatcct
     7081 tctggccact acgaggagaa ccccgaccat tacgtcaccg cccaggccgt cgaggccgcc
     7141 tgttggatgg cgggcatgaa caaggactat cccgagcact tcgccgcggg tctccagccc
     7201 cacggtgtgc gcgagcgtta ctactacgcc cgcggcccgc aactggtgaa ccgcgtgatc
     7261 gacatcagtg gcgtaattga ccggaaggtc gaggtgaacc tcgccaacaa ggcccaggga
     7321 cccgccggag aaaacggcgc ccgcctccgc gcgcgcctcg cgtcacaaaa cctgcgcctc
     7381 ccaatcctcg gtgactccga cgacaccgcg aaccggcagt acacgaagca cttcgtcttg
     7441 gcacgcgacg ccgagtgggg gaaaaaatat gggctggagt acgcggaacc gtatcactac
     7501 atcggccccg agccaccagt cctggaggac tacatcaaga agaacgcgat gcctctgtag
     7561 ggcaggcggc ggaggccaga cgccggccgg aaacccggtg aaccggtccc agctccgaaa
     7621 agagagcgat gggcgggaac ttcttgccgc tgaaaaccaa tgcaggatga acaccaggac
     7681 caccgtaatg gagccgagca acgaacaact gtactgccgg acgcgaaagg gtgatcaggg
     7741 cgccatggag acgctctacg agcggtgcga gcccccgctg taccgctacg catttcagct
     7801 ttctggcagc cgcgccgtgg ctgaggaggt cacgcacgaa gcctttctac aaattatccg
     7861 gcccgagtcg cgattcaatc cggaccgcgg ttcgctcgag gcgtacctgt acggaacagt
     7921 gcggaatcta atccgggcgg cgcgaaggag ccaggctgtg gaagggattg aggagcctgc
     7981 cgcggaagac gacattttgc gcgctctgat cgacgaggag atgagcctgg cgttgcactc
     8041 ggccctccag gaactgccga gccctttccg cgatgcggtg tttctgtgcg acctggagga
     8101 gcggagttac gaggaagcgg cgactacctt gggctgcccg gtgggaacaa tacgttcgcg
     8161 gttgcaccgg gcgcggcagt tgctggcagc gaggctgaag ccgctcaggc tatcggccgt
     8221 ttcaatagcg aggtaagcaa cgtggatcgc aaggactggt tggaagacga agaagtgctg
     8281 caggaaccga ttcgcagact gcgggcaatg ctggcggagg agcggtcgcc cgagtggcgg
     8341 aggcagcggt tgatggccga gttcgtcagg ctacatcgac ggtcgggcgg ttggctggct
     8401 tgggcgactc gcgcggctgc tgccgccttg ttggcgcttg ctgtgacact ggtctggcgg
     8461 ggcgctccgg agagtgctca gcgcaccggt atagaggtgg ctcagcctgg tatctggaac
     8521 cacgtgctgg atgggtcgat ggacgagaat gggtttgcgc cgatcccata cgccatgccg
     8581 ctagccgccg gagagtgcat acgggtgatg cgcacggagg ttaccggggc ggccctcgcc
     8641 cagatgggca tgagcctccc ttggggttac gaggacgact tcgacgtgga tatcctgttc
     8701 ggcgacgatg gatttccgcg agctgttcgg atggtctcca atacggagat ctgaggattg
     8761 agagggagga tgaagtccat gaacaagacc ggactagcta tttttgcgac actcacggcg
     8821 gcggcgattc tatcggcaca ggccacaccc ccgggcggtc cggtgcgcgg gccatttggc
     8881 gtggtgggcg gcgtgccagg agggggtgcc ggtgggtcca ttgtggtcgg gccgaagccg
     8941 gacatgctga tccccggcgc gccattctcg ggcgtgcagg tcagggaagt gaggcagagt
     9001 ctgccgaatg ggaaccagat ccagcgcctg gaagatacca agttttaccg tgacgggcag
     9061 ggacgtatgc ggattgaggt gccccagccc aaccccacca atgaccgggc gccggtcctg
     9121 atcgagatct tcgaccccgt agcgggcgct gcctacatcc tggatccgtc gaacctgaca
     9181 ggcaggaagt ctgcttttct gctgcccaag acacagggtc gttcgacgcg caagcggcag
     9241 gcaggtcaag tccaggaaga agatctcggg atcaggaagg tcaatggctt cgaggcgacc
     9301 ggaaggcgcg ctacgcagac gattccggcg ggcgcaatcg gcaaccggca gacgatcctg
     9361 agtgtgacgg aaacctggac gtccacggtt ctgaagattc caatactgca taagcgctcg
     9421 gacccgcgct tcggtgactc tacggtgcaa ctgacccaga tcgcactcgg cgagcccgac
     9481 ccctctctgt tccaggtacc ggagaactac aagctgaccc caggtggacc gttcgggcca
     9541 ggcatcgtgg gtggcgtaaa ggcggccaca aaaagcccgg cgcgcgagcg ctcgaaagac
     9601 tgaccgcggt gcccagtttg gcctccctcg gcgttggaga ccggtttcca gcctgccgct
     9661 gagccaggtc accggcaata cttacacaga agattgatct caagtccttt gcaatcagta
     9721 ctgatgtttg gaccggcgtt tgcatagatc actcacaggc ggacctgggg tgaagggaaa
     9781 gactgggatt aaccgccaga ggaggaaaca tgttgtggtt gatcttctgg attcttctcg
     9841 cgatgtggtt gctgggaatg gtgacctctt acacgctagg gggcttcctg cacatattgc
     9901 tgatcctggc agttgtcgca gttctgattc gggttatcca gggcaggaat cccgtctgag
     9961 catctcattg ctctcgttga ccccccaagt tcatcgaaat tccgggcggg cccccgtcca
    10021 ggtgctcgag aattttaagg agatgcgaaa tggctacgca accagctacg aagccagtgg
    10081 agcaggatct gctcgatctg gagaagcaat actggcaggc ggttaaggac aaggacgtgg
    10141 acgctgcgat gcacctcact gacgaccagt gcctcgtaac cggcgcgcag ggcgtggctc
    10201 ggatcgacag ggaaacgctc gcaagcatgt taaaggcgac gccgtacaca ctgaacgact
    10261 ttgaggtgag tgacgatgcg cacgtgcggc tgctacgcga cgatgtcgcg atcgtcgcct
    10321 acaaggtgcg cgaagagcta acagtggatg gtaagagcct gactctcgag gcagccgacg
    10381 cttccgcttg ggtacgccgc gacggacgct gggtctgtgc gctgcacacc gaatccatca
    10441 gtggcgatcc cttcggtcgc aacgggcgtc gcaagtctcg ccggagttct cgccacgtcc
    10501 ggaggagtta attggcaggt ccggagaccg cgctgcgtcg gcgcggtctc cacttcagtt
    10561 tttgataggc tcgtaggggc acacgagcag ggtgccccta cgtcaggcct acctaaaaat
    10621 gaatacggcc cgtgtactgc acttcgcggc cgtagtttcc gcccgctata gatgtgctct
    10681 tgccgaacgc cgagctggtc acgctcatgt tcggctggct gggcatgaac gagttcggga
    10741 agttgtagaa ctccatgcgg agttcgaact tcacccgctc ggtgatttgg aagtacttca
    10801 ccgccgtggc gtcccactgc cagaagccgg gacctcgtag cccgtcgtag taccacggat
    10861 tcgtgcgcgg cgtgtaggcc ggcagaaccg agcacttcga cgtatcgaac cactggccgc
    10921 gggtcgggtt gtcgatcttc ggatcgccag tgcaattcat cgcgccgaag gtcagttgcg
    10981 gaccgccgtt ccacatgaag atgtggctcg tggcccaccc gcccaccaga gcgtccagga
    11041 tccggttcgc gccgctcagg tactgccggc ccttgccgaa cggaagctca taggtgcccg
    11101 cgagacggac atagtgccgc gggtcgcgcg tgtcgatcat ggtgaagttc ttgttgtacg
    11161 tcgcaatcga gtcgaagaac tcgtcacgag tttcgtggct gtagttgtaa ccgaagctga
    11221 aggtcagtcc ctgagccatc gaccgctccg ccttgaactg caaggaataa tagtggttgt
    11281 ccttgtccct caggaagcgc tccgtcagcg tgccatattg tggatacggt gccagcaggc
    11341 tgttcacgct gaccgtcttc tgcgtgcgga gcgatcccgg catcttgtcg gccggtagga
    11401 gcatgtagaa cgggttcgct accgctttcg tagtggtgcc cttgtacgtg tagccgtagt
    11461 tcgggtccac catgttcagg ttgtagttgt agtctccgcc ccacatgctg gcgtcttgca
    11521 cgttccgacc gaggttcatg aagaacgtcg tgtccgtcag gatccggaac ggagcctgcc
    11581 gttgcagcga cacgttgaac cggtcgttga tcggatggat catgtcttgc gggaagaacg
    11641 ttgcgttggc ccccaggttt gtatagcgtc ccaacgactt accgatcggc gtttgcagcg
    11701 ggttcgaaac cgggaaagga tcgctgatga tcgtctgcgg cttgccttcg atgggtgaaa
    11761 tgatcggcgt gctccgcgcg aatccatcct tcggccagga gttggtttcc ggatagatgg
    11821 tcacccacgg caccgcatag cgggcgaagc cgacccggag cgctgtgttg tcattgatcc
    11881 ggatcgccgc gccgattcgg ggcaggaaga cgttcttctg agagttgaac actcctgggt
    11941 ggtcgttgtc cgtgaatacc cacgccccgt tccacttgta cggaatgctg gcgatcgcag
    12001 ttacctccgc tggcatcgtc acgccgccgg agagttccgg aatcgggttg gtcaggtcga
    12061 ggtatcgcga gtagagccgg ctgtactccg aaggcgccgt ctccagctcg tagcgcaatc
    12121 cgaggttgag ggtgacgcgg cgcgtcagct tgatgtcgtc ctggaagaag aaaccccaca
    12181 tctgctggtg ggagtccacc atcggggtaa tctgcgcgtt cccggtgttc gccacgccga
    12241 gcagcatcga tgcgtacatg tcgccgcttg tcgccgcgct atagccaaga aacgacttcc
    12301 cggtgtcgat cgagttgaag gtaaaagagc ccggacccgg gtttccattc tggtcgtatt
    12361 gataacgcag cgaccagccc gccttcatgt ggtggatgcc gcggtcgtgc gtgacgttca
    12421 ggttcgagtt gtgcgaccgc ccacggacca gccaccaact tccgaacccc gtataggccg
    12481 agccgttgcc gctaaagttg taccgtgggt aataaatacc gggcaggcct tccagcatcg
    12541 gcttgtacca gccgttggga tataacccgg cccatacgct ctccggtacc tgcgaccagg
    12601 ccgagttgta gtcgtcctcg agataggtcg cgccatagcg catattcact gttgtgctcg
    12661 ggctgaccat ccacaagaag tcgatggctg ggttcagcga gtccatcagg ccgccgttgt
    12721 ctgaaggaac ggcaatcgtg ccgccccagt tcgggttgtc cagccgcgtc tcgtacttcg
    12781 agaatcgcgc gtacatgcgc cacttgtcgt tgatgttgta atcgacgcgg ttggaaatgt
    12841 tccagtactt cagccaccag gcgtaggaga tcaggtagtt gttcatcccg ctggcatcga
    12901 ttccggggcc attgggcttc cataggtcgc tcgtgatctt cgccgacgtc ggattgatcc
    12961 ggtcggacgg aatcttattg ttcgtgaacg gcgtccgggt ggctgtgcct gtggccgggt
    13021 cgaacaccgt cgagaacgga tcgtagatca tccgcaggct gccgtccttc tggaaggtct
    13081 gcgagaaatc gcccgtccgc tggaggtcgg tcggcatcgt cagctccttg ctcgacggct
    13141 gcgtcgactt ccattgctcg tacgtctggt agaagaacag cttgttcttc acgatcgggc
    13201 cgcccaccgt gccgccccag atgtggtttt tcaccacgct gaagccgccg gtgacccggt
    13261 tggtcattgc gttcagtgtc gggttgcgtc cgaaataata cccggtgccg tggaactcgt
    13321 tcgtgccgga tttcatcgac acgttcagcg tcccgcccgc gctgaagccg aactcggcgt
    13381 ccagcgcgtt ttgctgcacc gccacttcct gcacggcgtc catcgacggc atgtaggaac
    13441 cgcgggccga aacaccgagc ggtacgccgt ccagcagcat gtcattcttg ccgcccgtcc
    13501 ggccacccac atcgaggccg ttcgacgacc acatgtagaa cgggttgcgg tgcgccacgt
    13561 cccagtaccg gttgattacc gccgggttca gcagcgccag cgtgaagggg ttccacgcca
    13621 gaatcggaat gtccttcaac atgttgcctt gcacggtcgt cgtcatggtg ctggtgttga
    13681 actgcaccgt ggccacgttc gcggtgaccg ttaccgtctc cgccactccg ccgatgacca
    13741 gttgcgcgtt caccgtaacg tcgccgcgcg tcagaacggc gatgttctcc tgaaggtacc
    13801 ggttgaagcc ggacatctcc actgaaatcg cgtacgtgcc tggctgcacg aaatcgaaga
    13861 gataccggcc cattgcgtcg gtctctctcg atgtctcaat gccggtattc atgttccgga
    13921 gcgtcacctt cgccccgacc accgccgcct ggctggggtc ggtgacaaaa ccttgcaccc
    13981 tggccctgta ttcctgagcg ctggccacac cgaccgccac gcagaggatg acgacgagcc
    14041 agacaggcgc aaatcgaaaa gaggaaaact tcatctattt gcctccttgg ctttcagaaa
    14101 actgctctct gtttgcaggg atgaccaccg gctccgaccc cagttgctta ccggtgggag
    14161 ggaatttctg tctgatcgct ttgccctgtc gaatatcacc caccctgacg tgggtttgtc
    14221 gctcaggact tcggtctgcg gcacaatagg aaagtgcaaa tggggcgcgc ggtggtcctc
    14281 ttccgtcgtt tcgcgtgggg ggttctcgga tacacgctgc tggtcgtgtt atggggcgct
    14341 tatgtccggg ccagcgggtc gggcgccggc tgcggcagcc actggccgct ctgcaacggc
    14401 gaggtcatcc cgcgtgcgca gcaagtggaa acgctcatcg agtttaccca ccggctcacc
    14461 agcggattgt cgctgcttgc ggtcgcggcg ctctacgtct gggccttccg tcttttcccc
    14521 cgtggcagcc gagtacggcg attcgcggtc ttgtccgtgg tgttcctatt gatcgaggct
    14581 gccttgggag ccggattagt gctcctccgt tacgtcgcac aggacgcatc gtccggccgc
    14641 gcgatctacc tctccgccca cctcgttaac acccagatcc tgctggcgat gctcgccctc
    14701 accgcctggt tcgcaactgc gcagccttcg cgaaattggc tgtctgaccc catttccctg
    14761 accgccctcg ccatcgctct cgccgtcagc gtcaccggag ccattgccgc cctcggagac
    14821 acattgttcc ccgccacctc gctggcggct ggtctgcgcg acgagctctc gggtgactcc
    14881 cacgccctgc tccggctacg gctccttcat cccgccgccg ccatcgccgg tgcggcattt
    14941 ctgctctgga tggccctgcg cgccattcgt tccgatatcg gccccgccgc gaaaaccgcc
    15001 gcccgctgga ccgccctcct cgtcttcctc caactcatcg ctggcgccgc gaacgtcgcc
    15061 ctgctcgccc ccatctggct gcaactgacg cacctgctca tcgccgacct cctctggctg
    15121 gccctcgtcc tgctcgccgt gtccgccctg caacccgcgg acgcgaagag cgccgtcacc
    15181 cgctagtcct acgatctgtg accgcggtgg tatagactac atccgttggt aaaccaaacg
    15241 tggtcacgaa cgatgcacac atccagagtt cttatcgcga tccttactgt tgtcgccctc
    15301 ggcgctgcgg gctgcggtgc cggctcctcg ctacgtacgt ggtacgtcga ctctctaacg
    15361 aaagtgttcc cggatgataa gccgggcacg aacgaacggt ccggcaaaac ctggctggtg
    15421 gcccgaaacg gccacgtcag cgtccagatc gccctgcggg cggacaaggc gattccgcaa
    15481 ctccgggccg aactcaaggc ccccgagaat tcaggccaga aactcactgc cgaagcgcgg
    15541 tgggtcgagt acgtccccgt cggctccaat ccgcccagaa caccctacga cgaagtcgtc
    15601 cgcccggctc ccagcctgtt cccggatccg ttgatggaga aatggccgtt cgatctgaaa
    15661 gcggcggcca cgcaacccgt gtggatcacc gtctatgcgc ccgcggacgc gccgcccggc
    15721 gagtatgacg gcgaggtgac gttcttcgcc ggacagaacc gcctcaagtc ggtggatttc
    15781 gcggtccacg tcactgcggc aaccgttccg gccgaacaga agctgaaagt caccaactgg
    15841 ttcacgttca ccggagagcg cctgcacaac cactacgaag tcgaagacta cagcgacaaa
    15901 tactgggaga tcctctacaa cttcgggcgc gtgatggcgg accacaagca aaacgtcatc
    15961 atcacgccga tcatgccgct gacgaagcca tcgatggcgg gcgggaccat gcgcttcgat
    16021 ttctcgctgc tcgacaagtg ggtcgaaacg ttccagaaag ccggcgtgat cggaaccatc
    16081 gagggcaggc accttcttgg acgcggcggc ggctactact cgccggtcgt cgtgcaggct
    16141 tacgtcgtcg agggaggcga gatcgtgcaa aaaggactcg accccgacga tccgcgcgcc
    16201 gaacaattcc tgaacaccta tctgcctgcg ctgtacgccc acctcaaaga aaagggcctg
    16261 acggaccact acctccagca tgtgctcgac gaaccccacg accgcgaggc ggccatctac
    16321 aaccgctacg cgaaaatcgt aaggaaacac ctccctggcg tcccgaccat cgacgccgtc
    16381 ggattggaac aggacatcag ctttttcgag gacgtctgcg acatctgggt cccggttctc
    16441 agcagcttcg accaccagct cgacaaaatg cgcgcccacg tcgagaaagg cggcaagatg
    16501 tggtactaca cgtgtgtcag tccccagggc cgctacctca accgcttcac cgacctgccg
    16561 ctcgtcaaga cccagcttct gcactggttc aacttccgct atgacctcac cggcttcctc
    16621 cattggggcg gcaacgcctg gggaccccgt ccgttcatga atgtccagac cgtcatcaac
    16681 cgcaaccgca cgctgctgcc ggcgggcgac aacgccatcg tgtatccgaa cgccgagaag
    16741 ctctccgtgc tcagctcgat tcgcctcgaa gcgatgcgcg agggcatcga ggattacgag
    16801 ctgctggtgg cgctggcgga caaagatcag gcgaaggcga aagggctggc gaagaccgcc
    16861 atccccaaca tcaatgatta catccgcgac gtatccgctt tcaggaacgt aaagcggcaa
    16921 ctcgtggaag cgttctaaag ggggaagtaa ggtgatcaag agagccactt ttggtgcact
    16981 cccggtcgtt cttggactcg ctctgatcct gtgcggctgt tcgacgcgcc cggcgctgga
    17041 ctactggttc gtggactcgc tggtaaaggt gtttc
//
LOCUS       DATIFQ010000294         2202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1818982, whole genome shotgun sequence.
ACCESSION   DATIFQ010000294 DATIFQ010000000
VERSION     DATIFQ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818982"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VLE22_13895"
     CDS             complement(<1..363)
                     /locus_tag="VLE22_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15545.1"
                     /translation="MADGFQVTGTNAGALFTLKLHRGDGMTLVAMNWKKGKPPANFVG
                     FAIEYQEPEGDRFYALKNRLCFPDGGRQADPNRFSTLRSPIQKFRWVHFPRNAELSGA
                     FTYRVTPVFMNEQDELSYG"
     gene            complement(646..>2202)
                     /locus_tag="VLE22_13900"
     CDS             complement(646..>2202)
                     /locus_tag="VLE22_13900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSB15546.1"
                     /translation="NVANLLLARAVSRQKEFSIRLALGAQRSRLTRQLLIETLLLAAG
                     GMVVGVTLVLWMGQLLSFMIPVADVPLDFGGGLSLPTLGFTLLIAVVATLLSGTVPAL
                     LTARSDLNETLKEGGRSGGAGSGSHRLRGLLVVGEMALVMVALIGAGLFYRSFKNASG
                     IEPGFDRTNISVSQFYLSYAGYSAREQRDFCRNLRERLESTPGLVGVTYSDVIPMSTA
                     SGAGSTPWHQLDVEGYTPARDEQMMIHRATVPPGYFNLLGIRMLEGRDFTERDAEGAP
                     MVIIVNETFAKRFFQGRNPIGRKIDCEGNQATVVGLVKDSKYHTPIEGPTPFFYIPFR
                     QWFAPGLNFSVFIKSAGDPLLMTPVLRREALALNQDAIFTTRLLSDATTGSLFAQRTA
                     ASLLGVIGGISLLLAAVGLYSVMSYAVSQRTQEMGIRMALGAQPSDVLGLVLREGLRL
                     TVPGLLAGSVIALAAARIVGGMLVNVSASDPLTFASAATFLVLVAALASYLPALRAMR
                     VDPVVSLRNE"
BASE COUNT          434 a          683 c          703 g          382 t
ORIGIN      
        1 cccgtagctt agctcgtctt gctcgttcat gaagacgggc gtgacccggt aagtgaacgc
       61 tccggagagc tcggcgttgc gcgggaaatg cacccaacgg aacttctgga tcggcgatcg
      121 cagcgtggag aagcggttgg ggtccgcctg ccggccaccg tccgggaagc atagccggtt
      181 cttcagcgca tagaatcgat cgccttctgg ctcctgatac tcgatcgcaa acccgacgaa
      241 attcgcgggc ggcttgccct tcttccagtt catggcgacc agggtcattc cgtcgccacg
      301 atgaagtttc agcgtgaaca gtgcaccggc gttggttccg gtgacttgaa aaccatcagc
      361 catgtgatcc tccttcttgt cgctttccag ccttgtcttc accgacaggt cgggccgagg
      421 gccgctggtg aaatgcgggg ggcggcccag cgcagcacct cgcgggcggt gctgaggagc
      481 cacttccggg gaacacctcc ccggcggtct ctggttgttg gtgaatccag ccgtgggttt
      541 gtttcaatga aaaagggtca aatcgccact tgacagggca gcggcaacga gtcacaggtt
      601 cacgacctcg actacctgcc ggagtgcgat gtcttgccgt gcctgctact cgttcctcag
      661 cgagaccacg gggtccacgc gcatggcacg caaggccggc aggtagctgg caagcgcggc
      721 caccagcacg aggaaggtcg ccgcggacgc gaatgtgagc ggatcggaag cgctgacgtt
      781 caccagcatt ccgccgacga tgcgcgccgc cgccagggcg atgacgcttc cggcaagcag
      841 tcccgggacg gtcaatcgaa ggccttccct cagcaccagc ccgagcacgt cggatggctg
      901 ggcgcccagc gccatccgga ttcccatctc ctgcgtgcgc tgactcacgg cgtagctcat
      961 gacgctgtac aggccgacgg ccgccagcag cagcgagatc ccgccgatca cacccaacag
     1021 gctggccgcc gtgcgctgcg cgaatagtga gccggttgtg gcgtcggaca acaaccttgt
     1081 agtgaaaatt gcgtcctgat tgagcgccaa ggcctcccgg cgcaggacag gggtcatcag
     1141 tagcgggtcg ccggcggact tgatgaagac cgagaaattg agaccgggcg cgaaccattg
     1201 gcggaatgga atgtagaaga agggagtcgg gccctcgatc ggcgtgtgat atttgctgtc
     1261 tttgaccagc ccaaccaccg ttgcctggtt gccttcgcaa tcgatcttgc ggccgatagg
     1321 gtttctgccc tgaaagaacc gtttggcgaa ggtttcattg acgatgatca ccatgggcgc
     1381 cccctccgca tcgcgctcgg tgaagtcgcg gccttcgagc atccggatgc cgagcaggtt
     1441 gaagtagccg ggcggaaccg tggcgcgatg gatcatcatc tgctcatcgc gcgcgggagt
     1501 atatccctca acgtcgagtt ggtgccaggg cgtggaaccg gccccggaag cggtggacat
     1561 cgggatcacg tctgagtatg ttacgccgac taagcccggc gtcgactcta accgctcgcg
     1621 gaggttccgg cagaaatccc gctgctcccg cgcggagtag cccgcgtagg agagatagaa
     1681 ctgagacacg gaaatattgg tccggtcaaa acccggctcg atgccgctgg cgttcttgaa
     1741 actccggtag aagagccctg cgccgatcag agccaccata accagcgcca tttcgccgac
     1801 cacgagcagg ccgcgcaggc ggtgcgagcc ggaaccggcg cccccgcttc ggccgccctc
     1861 tttgagggtt tcattcaggt cggagcgggc ggtgagcaga gccggcaccg tgccggacag
     1921 caaagtcgcg acgacagcaa tcagcagcgt gaaaccgagg gtcggcaggc ttaatccacc
     1981 gccgaaatcc aacggaacgt cagcgacggg aatcatgaac gagagcaact gccccatcca
     2041 caaaaccagc gtcaccccaa ccaccatgcc gcccgcggcg agcagcagcg tttcgatcag
     2101 gagctgcctc gtgaggcggg aacgctgggc ccctagcgcc agccggatac tgaactcctt
     2161 ctggcgggat actgcgcggg cgagcagcag gttggcgacg tt
//
LOCUS       DATIFQ010000295         5546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1245200, whole genome shotgun sequence.
ACCESSION   DATIFQ010000295 DATIFQ010000000
VERSION     DATIFQ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245200"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VLE22_13905"
     CDS             <1..1195
                     /locus_tag="VLE22_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB15547.1"
                     /translation="AVLKQGYEITEDAEGARWVREQMVARAPHGFRTADMVIQRWMSD
                     RPRPDHEDSRERVSNYYRSLYEATGDWVKQWPLYSAPWSLRFRALRELPDESASELVS
                     AGEGLLNAEIKNASNYGVPPRIEVAEAMIERFVPSFILNNARYNQKQAFWKMQLIRVR
                     RAVARDDVSGAEAALKEMEAVLKKREPEDPQMFWLSRPHMVDAYLAARELAKAKTVLA
                     TMSDWLAAHKAAPEDGAAIRSYAQREADYWQRIGFGWTRVKRALPAFKLTDLKGKQWT
                     IKDFEGRRTFMNFWATWCGPCRAELPHVQKLYLRLKDRSDVQVVTLNVDEDQSVIGPF
                     MSREAFAFPVVPARAFAQDELGIQGYPTSWIVNTEGVVELEQSGFAAEGDKWLDDVVA
                     RLSAR"
     gene            complement(1596..2552)
                     /locus_tag="VLE22_13910"
     CDS             complement(1596..2552)
                     /locus_tag="VLE22_13910"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HSB15548.1"
                     /translation="MSGFPLFDTPASFDRAGLAEKLRRLASEHVWLGTSSWKYEGWLG
                     QIYTRERYLTRGRFSKRAFEAECLKEYAETFPIVCGDFSFYQFPSEEYWRKLFLSAPA
                     ELRYALKVPEEVTVKVFPDHARYGARAGLENPTFLDTHLLEDAFLRPLEPYRSQLSAL
                     IFEFGTFSQKNYETAQRFVEDLDQFLAALPAGFRYSVEIRNPEYLGPEYLGCLRAHNV
                     AHVLNAWDRMPGVGAQLAVPGIFTSDFTVCRALLRRGRAYAKAVELFTPYDRVQEPLP
                     QTREALRELIRRAREHRIEAFIFVNNRLEGNAPETIRAIVED"
     gene            2573..3331
                     /locus_tag="VLE22_13915"
     CDS             2573..3331
                     /locus_tag="VLE22_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB15549.1"
                     /translation="MLTPRQQSIYDFILDYRRRNGCTPSIPEIQREFGIRSPNGVAGH
                     LLALQAKGLIRRATRGSRKIDVTGPFDSRKSPVHSGPRGSPACSLESMSELAELLERF
                     RRGAELIAVAITGAAGAELDYSPAPGRWSIRQILCHLADSEIVVADRFRRIIAEENPT
                     IISYDQDAWSRNLDYNRRKISQALETFRRTRAENYELLKPLPPETWERIGTHSEHGKV
                     TLLDLLRIYAEHAEGHSLQLREIREQYKVFKARS"
     gene            complement(3342..4169)
                     /locus_tag="VLE22_13920"
     CDS             complement(3342..4169)
                     /locus_tag="VLE22_13920"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HSB15550.1"
                     /translation="MRRRTFLASLSALAGTKAVQSERSRLRVLVITGQTDLPYHDWTK
                     TTPFLKRVLESTGRFEVTVLDAPKTLSASMLAPYQAVILNYNGPRWGAEPEKALEDFL
                     RAGGGMVAFHGVSYGAFLGMEQDAKGRFQWSTKPDAEWKAYKEMIGTTWEPANIGHSA
                     RHEFRVKFADPDHPITRGLGPDYLIDDELYHKMAHMPGIRVIMTAYSDPAKRGTGKDE
                     PIAWTVSFGKGRVFHTTNGHDLRAINTWGFLTTFARALEWVATGDVTLTGRIDPIMQ"
     gene            4257..>5546
                     /locus_tag="VLE22_13925"
     CDS             4257..>5546
                     /locus_tag="VLE22_13925"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HSB15551.1"
                     /translation="MYRMYRLFLIAWIAATASAAAPPAWVERSNRNAKILLDVQARFA
                     PEAAARTGVEGIDREVVDLKAGFRERSREATRKAIGALREKLDAETDARVRQDLEILI
                     RAAELDVKESVLSEKYTLPYFDVTRFVFGGIRSLLDDQIATDRRPAALVRLRRYAGME
                     EGYAPLAKLAEERIRERLSQPGLLAPFNGEIEKSLADSQFLLDGIEPLFRKFGVDDYE
                     EPLAKLKTQIAAYHDFVCREILPKARTDFRLPEELYALSLEQYGNDMPPAALAQRARG
                     AFLEIQGQMQEIAGQVAREREWSFAGYRDVIRELKKKQLVGDAILPHYEQRLAQIEEI
                     VRRENLVTLPGRPARIRIASAAESAAVPAPNMRPPRLIGNAGESGEFVLPLNVPDTSG
                     KMKSFDDFTFEAASWTLTAHEARPGHEMQFASIVEQGV"
BASE COUNT         1111 a         1719 c         1719 g          997 t
ORIGIN      
        1 ggcagtcctg aagcaaggct acgagatcac cgaagacgcc gaaggcgcac gttgggtgcg
       61 cgagcagatg gtagcgcgcg cgccgcatgg gttccgtacc gccgacatgg tgatccagcg
      121 atggatgtcg gaccgtccga gaccagacca cgaagactcg cgcgaaagag tgagcaacta
      181 ctatcgctcg ttgtacgaag ccactgggga ctgggtgaaa cagtggccgt tgtacagcgc
      241 gccctggagt cttcgattcc gagcgctgcg cgagctaccc gacgaatcgg catcggagct
      301 cgtctccgca ggagaaggcc tgctgaacgc ggagataaag aacgcgtcca actacggcgt
      361 gcccccgaga atcgaagtgg ctgaagcgat gatcgagcga tttgtcccgt cgttcatctt
      421 gaacaacgcg cggtataacc agaaacaagc gttttggaaa atgcaactca ttcgggtacg
      481 gcgggctgtc gccagggacg atgtttcggg ggctgaggcg gctttgaaag aaatggaggc
      541 ggtactcaaa aagcgtgagc ccgaggaccc gcagatgttt tggctcagtc ggcctcatat
      601 ggttgacgcc tatctcgcgg cgcgcgagtt ggcgaaggcg aagacggtgc tcgcaacgat
      661 gagcgattgg ctcgccgccc acaaggctgc accggaggat ggcgctgcga tacgttcgta
      721 cgcacagcgc gaagccgact actggcagcg catcggattt ggctggacga gagtgaaacg
      781 cgcactgccg gcgttcaagc tgacggacct gaagggcaag cagtggacaa tcaaagactt
      841 cgagggtagg cgcacgttca tgaacttttg ggcgacctgg tgcgggccgt gccgtgccga
      901 gctgccgcac gtacagaagt tgtatttgcg cttgaaggat cgctccgacg tgcaagtagt
      961 gacgctgaac gtggacgaag atcagagcgt gatcggaccg ttcatgagcc gggaggcctt
     1021 tgccttcccc gtcgtacctg cgcgggcgtt tgcacaggat gagttgggca ttcaaggcta
     1081 tccgacgtcg tggatcgtga acacggaagg cgtggtggag ctggagcaaa gtggctttgc
     1141 tgcggaagga gataagtggc tcgatgacgt cgtcgccaga ctctcggcca ggtaacccca
     1201 ctattctgaa caccggtcgg cgacgagtat gcgctcgacc gtgggcgctg aggaagtccg
     1261 aactccctcc accctcacaa ctccggtcac aatccatctc aaattcatta actcactctt
     1321 tcggaatgac ttatatccgg gtcacgccac cgcatcaccc caggattgcg atcccgctcc
     1381 ttaacattga cacgggaggc aaacggcggc cacccacttt ccttcccccg ggacccgagg
     1441 ttttccgatg gccccgaagt gtctgattcc gggattggct ttgttcagtc aacggtcccc
     1501 ggcaggaccg gccccggtcc ggaggacctt cggaaagaaa ctccgctggt cgaagatcgc
     1561 ttgttggatc cggtggcggc gcggcgcgaa acgtctcaat cctccacgat cgcacggatc
     1621 gtttccgggg cgttgccttc gagccggttg ttcacgaaga tgaacgcctc gatacggtgc
     1681 tcgcgggccc ggcggatcag ctctcgcagc gcctcgcgcg tttgcggcag cggctcctgc
     1741 acccggtcgt acggcgtaaa gagctccacc gccttcgcgt acgcccgccc gcgccgcagc
     1801 agcgcccggc ataccgtgaa gtcgctcgtg aaaatgcccg gaaccgcgag ttgcgctccg
     1861 acgcccggca tacggtccca ggcgttcagt acatgtgcga cgttgtgcgc gcgaaggcag
     1921 ccgagatact ccggtcccag atactccggg tttcggatct cgactgaata ccggaacccc
     1981 gccggcagcg ctgccagaaa ctggtccagg tcctcaacga accgttgcgc ggtctcgtag
     2041 tttttctggg aaaacgtacc gaactcgaag atcaacgccg aaagctggct ccggtacggc
     2101 tccagcggac ggaggaaggc gtcttcgaga aggtgggtgt caagaaacgt gggattctca
     2161 agacccgcgc gcgccccgta ccgagcgtga tccgggaaca ccttcaccgt gacctcctcc
     2221 gggaccttga gggcatagcg cagctccgcc ggcgccgaga gaaagagctt ccgccagtac
     2281 tcctccgaag ggaactggta gaacgaaaag tcgccgcaga cgatcgggaa cgtctcggcg
     2341 tactccttca ggcactcggc ttcgaaagct cgcttcgaga aacgtccgcg ggtcaggtat
     2401 cgttcccgcg tgtagatctg cccgagccag ccttcgtatt tccacgagct cgtgccaagc
     2461 cacacgtgct ccgatgccag ccggcgcagc ttctccgcca agccagcacg atcgaaagaa
     2521 gcaggcgtgt caaatagtgg aaagcctgac atgagaaact ccttatacgc ccatgctgac
     2581 cccgcggcaa caaagcatct acgacttcat cctcgattat cgccgccgca acggctgcac
     2641 tccatcgatc cccgagatcc agcgggaatt cggcatccgg agtccgaacg gcgtcgccgg
     2701 ccatcttctc gccttgcagg caaaggggct gatccggcgc gccacgcgcg gatcgaggaa
     2761 gatcgacgtc accggaccgt tcgactcgcg gaagtctccg gtgcacagtg gtccgcgcgg
     2821 tagtccggcg tgtagtctag aaagcatgag cgaactggcc gaacttctgg aacgcttccg
     2881 gcgcggcgcc gaactgatcg ccgtcgccat cactggggct gcgggagccg agctggacta
     2941 ctcgcccgca cccggccggt ggagcatccg gcagatcctc tgccacctcg ccgacagcga
     3001 aattgtcgtg gccgaccgct tccgccgcat catcgccgaa gagaacccga cgatcatctc
     3061 gtacgaccag gacgcttggt cgaggaacct cgactacaac cggcgcaaga tctcccaggc
     3121 tctcgaaacc ttccgccgca cgcgcgcgga gaactacgag cttctgaagc cgctgccacc
     3181 cgagacgtgg gagcgaatcg gcacgcactc cgaacacggc aaggtcacgc tgctcgacct
     3241 ccttcgcatc tatgccgaac acgcggaagg ccattctctc cagctccgcg agattcggga
     3301 acagtacaag gtgttcaagg ccaggagttg aacgccggca cctactgcat gatcggatca
     3361 atcctgcccg tcagtgtcac atcgcccgtc gccacccact cgagggcacg ggcgaacgtc
     3421 gtcaggaagc cccacgtgtt gatcgcacga aggtcgtggc cgttggttgt gtgaaacacg
     3481 cgtccctttc cgaaggagac cgtccacgcg atcggctcgt ccttgcccgt gccgcgcttg
     3541 gccgggtcgc tgtaggcggt catgatcacc cggatgcccg gcatgtgggc catcttgtga
     3601 tacagctcgt cgtcgatcag gtagtcgggg ccgaggccgc gcgtgatcgg gtgatcggga
     3661 tccgcgaact tgacgcggaa ttcgtgccgc gccgagtggc cgatgttggc cggctcccag
     3721 gtggttccga tcatttcctt gtatgctttc cactcggcgt cgggcttcgt ggaccactgg
     3781 aaacgtccct tcgcgtcctg ttccatcccg aggaacgcgc cgtaggagac gccatggaaa
     3841 gcgaccattc caccgcccgc gcgcaggaaa tcctcgagcg ccttctcggg ctccgcaccc
     3901 cagcgcggtc cgttgtaatt cagaatgact gcctggtagg gggcgagcat tgaggcggag
     3961 agcgtcttcg gggcgtcgag taccgtcact tcgaaacgtc cggtgctctc gagcacgcgc
     4021 ttgagaaatg gcgtggtctt cgtccagtcg tggtatggca gatcggtttg gccggtgatg
     4081 acgagcacac gcagccggct gcgctcggat tggacggcct ttgttccggc caatgcgctc
     4141 aaggaggcga gaaacgttct gcgtctcatt gcgtctatct cccgtacatg aaaactcgac
     4201 aagccaggag acctatcata cgctggaaga caagccacaa gaatctggac cactttatgt
     4261 atcgtatgta ccgtctgttc ctgatcgcat ggattgccgc aactgcgtcc gccgccgctc
     4321 cgcccgcctg ggtcgagcgg agtaaccgga acgcgaagat cctcctggac gtccaggcgc
     4381 gctttgcgcc ggaggcggct gcgcgcactg gagtcgaggg aatcgaccgg gaggtcgtcg
     4441 atctcaaagc cgggttccgt gaacggagcc gggaagccac gcgcaaggcg atcggcgcgc
     4501 tgcgcgagaa gctcgacgcc gaaaccgacg cccgagtcag gcaggacctc gagattctca
     4561 tccgggcggc ggaactggac gtcaaggaga gcgtcctatc cgagaagtac acactgccgt
     4621 acttcgacgt gacccgcttt gtgttcggtg gcatccgcag cctgcttgac gaccagatcg
     4681 caacagatcg ccgtcctgcc gcgctcgtcc ggttgcggcg gtacgctggc atggaggagg
     4741 gctacgctcc gctcgcgaag cttgccgagg agcgcatccg cgagcggttg agccagcccg
     4801 gtctgcttgc cccctttaat ggagagatcg agaaaagtct cgccgattcc cagttcctgc
     4861 tggatggcat cgagccgctg ttccggaaat tcggcgtcga tgactacgag gaacccctcg
     4921 cgaagctgaa gacacagatt gccgcctatc acgacttcgt gtgtagggag atcctcccga
     4981 aagcccgaac ggactttcgc ctgcccgagg aactctatgc gctctcgctc gaacagtacg
     5041 gcaacgatat gccgccggct gccctcgctc agcgtgcacg gggcgcgttt ctcgaaattc
     5101 agggtcaaat gcaggagatc gccggtcagg ttgcacgcga acgggagtgg agcttcgccg
     5161 gctatcgcga cgtgatccgc gagctgaaga agaagcaact cgtcggagac gccatcctgc
     5221 cgcattacga acagcggctg gcgcagatcg aggagattgt ccgccgcgag aacctcgtca
     5281 cgctacccgg ccgtccggcc cgcattcgga tcgcttcggc agcggagagc gcggcggtgc
     5341 ccgcgccgaa catgcggcca ccgagactga tcggcaatgc cggtgaatca ggcgagttcg
     5401 tgctaccgct gaacgtgccg gacacatcgg gaaaaatgaa atccttcgac gatttcacct
     5461 tcgaggctgc gtcgtggacc ctgaccgccc acgaagcacg cccaggccac gagatgcaat
     5521 tcgcctcaat cgtcgaacag ggtgtg
//
LOCUS       DATIFQ010000296        11271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1435778, whole genome shotgun sequence.
ACCESSION   DATIFQ010000296 DATIFQ010000000
VERSION     DATIFQ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11271
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435778"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(567..2705)
                     /locus_tag="VLE22_13930"
     CDS             complement(567..2705)
                     /locus_tag="VLE22_13930"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HSB15552.1"
                     /translation="MIRLTGLLLCASVAFAQAPEGLVLANNHVRLEFESGGMGLKAMV
                     DLKTGRNHIQAVEGKRLLWEATLGRGTLTRAANNNYKPCTFARIETLPGGVKRAVMEW
                     NDLRWWLEDKALTVRVTVDLPPDSGIAQWRIFVENHSDYWGLTRVAFPLVNGFPAPGE
                     YDVARPTFGEGGQLLRKWNERVQGRHPSGGWPMQFCALSRGRDSVYLGTRDPDGQAKN
                     FDIEPGKQTSVIHHPENLSEAGSDWPDTYPVEFGVYQGSWVAAAHRYREWALKQKWAG
                     AGKLSQRRVPALMKNAAFWVCESWLWKDPPKGEAPSTNMAEWVQDQSAAKGAQADPKE
                     LDAPLVAAQELMGVPTAIHWYNWHQTRFDNLYPHFLPAKNGFRERVKELVARGVLVMP
                     YINGSSADMNIADWEKFAPHAIVDEAGGFRPHLYSEAAGRLLGMCPSQLFWQGTIARL
                     VERLVNEEGVNGVYVDQISAMEHEMCFSRAHGHPLGGGRYWVDGNRELLRKVKNVAER
                     AGREVTITSEGADEVFLDLVDANLTWAQPTDWQIPLIQVVYSGYTLFFGSPCDYEQSD
                     TFFRFGQGQALIDGRQNGWMNLRMFDGKHQEKVRYLKECARVRASYGKYLTYGQLLEP
                     VEPLDTVTTFTEDVFGWNKKHRGSAPVAEGRLWEAEDGSLAAFFANYGDQEVTFRYRV
                     EPSRFGLKGAAVERTETLDPASVRVVEVGR"
     gene            2961..5363
                     /locus_tag="VLE22_13935"
     CDS             2961..5363
                     /locus_tag="VLE22_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15553.1"
                     /translation="MKRAAAVLLISIGPWLEFPAVVSAQAEAVAQGVVVAEADHSLLP
                     AAALVLEGAALPQPLGATTGTDGRFRFARLTPGDYSLTVTHDGFREQRYRLTLRPHEV
                     QDRTLEMALRPVEETVQVVSEANPLPSVHSPNSTFLAPDRLLLLPLTQRTNLPDVILQ
                     AAPGMIRGHDDFVHIRGHEVALSPFINGVSFWENPHMVFSPGLGADYIESVNVMTGSF
                     SAEYGNRFGGVLDVVTKSGFTADNHGSVTLGAGTALRHNASLEFGGHTKRFGYYLNAA
                     AFESARFLSPPEPNAMHDAGRGTRAFAQLDFNANDRNVLRLTLMGDGADFELPKTLLD
                     AQMRPDFDNFQRTRSQSAILSWDHIRSADTLIHMALYQRYSRVRALPNADPYGAKLDS
                     TRTLNTNGIKTDYSRFFRGHALKAGVDLVLLRPEEAFGYLSQPWITFTHLPDVSQDHI
                     HFRGPDMGPGIPRPVLFRQEKTGGQASVYLQDKIRLTRGLSADVGLRFDRHSLAISDS
                     HFSPRLNLGYAFRSGTVLYGSYNHYFVPPPVENVLASSAGLTSLISEIATPLPPLRPV
                     IENQFELGVVQPVAQRLRVGVTGYYRISNNAVHTVLFPDSRFYAYANFDKGKAYGMEV
                     KAEAPMVRALGLSGYFNYALGRVWFYNPVTAGFITEAEHLSETNRFLAPMDQTHTLTS
                     GINYHNRRLGLWTSMAFEYGSGTPGSHGGGDHEDAGDEADHEHAPVPGGCDVRCPEHF
                     TQNLSVGWDAVRKRERPHLTLQFNIENLTNNVYLISKESTFVQGQYSIPRLFSGSLKL
                     RF"
     gene            complement(5629..5781)
                     /locus_tag="VLE22_13940"
     CDS             complement(5629..5781)
                     /locus_tag="VLE22_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15554.1"
                     /translation="MGELAANVHGRRACLVQRMVDVDVALVAKGRFGEIPPLATERAE
                     ALESIP"
     gene            complement(5805..6305)
                     /locus_tag="VLE22_13945"
     CDS             complement(5805..6305)
                     /locus_tag="VLE22_13945"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HSB15555.1"
                     /translation="MPDKIETIPEKTRWEMATKGLTGAYIAISKALKQAVGQKKFEEF
                     NGSLWYEAGKGAKEFADTVGLATENAEDVERVTHLLAKASMGPEFVFEVVEATKDRCV
                     GKTTQCPWHKRWKEQGVDFDTCGVGHQRWGDGAAESLNPNFTFRLTKNMLRGDSHCEW
                     VVERKK"
     gene            complement(6575..6706)
                     /locus_tag="VLE22_13950"
     CDS             complement(6575..6706)
                     /locus_tag="VLE22_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15556.1"
                     /translation="MTEADIEIILERLVEGYAEHYSNVRLNSAIGYITPKDMLAGRQ"
     gene            7210..8769
                     /locus_tag="VLE22_13955"
     CDS             7210..8769
                     /locus_tag="VLE22_13955"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HSB15557.1"
                     /translation="MDRLPLEILGERPGRNEAGLAPSLAYASYIGGTGTDVVTAVAAD
                     ADGFLWVAGYTTSTDLPVTATAVAAASKGGRDAFVIKLDPRASGLSSLVYATYLGGAG
                     DEEPVAMVVRGNMVFLAGSTSSTDFPTAGNRYKDTNSGRRDSFVVKINTAVGGTAGLL
                     YSSYVGGENYEYAYGLAVDSNERMYVTGYTTSTTSFPVTANPLQASNRGGYEAFLYVI
                     DPNLGTGSSLVYSTYFGGSSTDIATGIALDASGKVYLTGQTMSDNFPVTGAAYQTGYH
                     GKGDIFITRLDLTKSGLAGLDYSTYFGGGNFETAYGIAIDPAGNVCLTGYTMSSDFPV
                     TPTAYQQSFGGLADVFVAKFDFTAAPGTMLYSTYLGGVGADIAYALSVGAAGRIYVTG
                     YAYSGGFPSAGPPLQEGSSGINGAFLSAVDTSASDAASLLLSTHFGDGGTVGYGLAAD
                     GAGSLHVAGLTISTALPVTEGALSTVPRGATDGFVMKVDLSEGQPLGVLSRNQSAAAP
                     RTPMRVISAPR"
     gene            complement(8688..10943)
                     /locus_tag="VLE22_13960"
     CDS             complement(8688..10943)
                     /locus_tag="VLE22_13960"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HSB15558.1"
                     /translation="MMTANGGGEKHHTDRDRPRWLVHLTGPAILFLLCTGFFWKIVLT
                     NQYTWLEGSPDLAHQVLPWFQFQAGEIHAGRIPLWDPYLWGGQSLIGQAQPGLAYPLN
                     WILFLLPLRNGWIRMVYLHWYFVLIHFMATLFCYWLCRDLKRSRAASIIAGLAFGLGG
                     YIGTTDWPQMLNGAVWAPLVFLFLLRATRGERPLANSALSGTFLGVAWLSGHHQIPIF
                     ITLAAGAVWLFFVLKSGRFEVKMVRFGAIFVLFFLLTSGLQTLPAYEYGKLAKRWVGS
                     SEAVGWKDPIPYNVHDFFSLRATSLLGIVIPGHHRHTDPSIGLVALSLALLGVALSWK
                     SLPVRIFGAIAAGGLLFSLGRNNVFHGILYSIIPAVEKARNVSTAVFIFHFGIAVLVA
                     YGIDSYLSSEHAPWQRRMWIALSVFGAVVFTILAVLVLAQKPPGDDRFVVNAFVALLF
                     AMLLYGWRRGALSQTAACVLTAGLMLIELNNFSGYYWPNRDDKSRPMHLRYLEGLTKN
                     ADLAQVLRTQPWPVRFEVPTEEIPFNFGDWYGIDQYGGYLASLPVNLVRFDQFNARIV
                     KMFGVNYSVTRKPPGPNQQEVFTAKSGLKLYWNTDAFPRAWAVHEAVQAHNNIEAIGF
                     INDGNFDLRKRVFLLSSPPQLETCNGDESVRMLKRTSGSLTVEADLKCRGMVLVGDSY
                     FPGWRATVDGKPAKIFEANGMIRGVVVEAGRHTIEMKYRPWSVYLGALMTLMGVLGAA
                     ALWFRERTPRG"
     gene            complement(10963..>11271)
                     /locus_tag="VLE22_13965"
     CDS             complement(10963..>11271)
                     /locus_tag="VLE22_13965"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HSB15559.1"
                     /translation="IERYEPRAIQLRVQAHGRGLLVLSELFYPGWRAEVNGRDVGIVE
                     VNRALRGIPVPAGESRVVVSHAPHSVAAGAALTLASFCALPFALVCLRRSRRLSRVTP
                     "
BASE COUNT         2144 a         3760 c         3147 g         2220 t
ORIGIN      
        1 cgaggcggtg aaaaggagac gcttcgggaa atcgtaaaag ggatcgaatc caatcctgcc
       61 gtgctctctc tcaagaaacc gttcgatctc ggtggtggta acaaggccat cgcaccagag
      121 gactgggcgg acgcacgtgc ctgggagcta atcaaacaga tggcggcgtc gaaatagttg
      181 agatcgccct ggagccaacg acaactcggc ggtgggggtc atccgctctc gggtagcggc
      241 taccacgtct cgatttcttt gccatataca gaccgaaatt tggcacgccc aatattcccc
      301 ggaccgggtt ttgccgcact cgtcaacaag catccgtcga gcacgccgct aaggggctta
      361 cctcctggac gcagttcgtc tcgatgctgt tttgccaacg cggtcgcgcc gactccatgg
      421 gcgagttctg caccggattg ccctgctgtc tggggccgct tggtgcattt gggcatcgcc
      481 aaggcgcccg gccattcgac gctgtcctat gccaacgaac accgcgccac cgataatccg
      541 cagtactgcc gatagactcg gagctcctac cgccccacct ccaccaccct caccgacgcg
      601 ggatcgagcg tctccgttcg ctccaccgcc gcacccttga ggccgaacct cgatggctcc
      661 acgcggtagc gaaaggtgac ctcctgatcg ccataattcg cgaagaaggc cgccaggctg
      721 ccgtcctccg cctcccacaa gcgcccttcc gccaccggcg ccgacccacg atgctttttg
      781 ttccacccga atacgtcttc ggtgaacgtg gtcaccgtat ccaacggctc caccggctcc
      841 agaagctgcc cgtacgtcag atactttccg taactcgctc tcacccgcgc gcattccttc
      901 aagtaccgca ccttttcctg gtgcttcccg tcgaacatcc gcaggttcat ccagccgttc
      961 tgccggccat cgatcaacgc ctgcccctgc ccgaaccgga agaacgtgtc gctctgctcg
     1021 taatcgcacg gactcccgaa gaacaaggtg tacccgctgt aaaccacctg tatcagcgga
     1081 atctgccagt ccgtcggctg cgcccatgtc aggttcgcgt ccaccaggtc gaggaacact
     1141 tcatcggcgc cctcgctcgt gatcgtcacc tcgcggcccg cccgctcggc cacgttcttc
     1201 accttccgca gaagctcccg gttcccgtcc acccagtacc gcccaccgcc cagcggatgc
     1261 ccgtgcgcac ggctgaaaca catctcgtgc tccattgccg agatctggtc cacgtacacc
     1321 ccgttcacgc cctcctcgtt gaccagccgc tccaccagcc gcgcgatcgt cccctgccag
     1381 aaaagctgcg acgggcacat ccccagcagc cgccccgccg cctccgagta caaatgaggc
     1441 cggaacccgc ccgcctcatc gacaatcgcg tgcggcgcga acttctccca gtccgcgatg
     1501 ttcatgtccg cgctcgaccc attgatgtac ggcatcacca gaacgccccg tgcgaccagt
     1561 tccttcaccc gctcccggaa accgttcttc gccggcagaa agtgcggata caaattgtcg
     1621 aaccgcgtct ggtgccagtt gtaccagtgg atcgcggtcg gcacgcccat tagttcctga
     1681 gccgccacca gcggcgcgtc cagctccttc ggatccgcct gcgcgccctt cgccgcactc
     1741 tggtcctgca cccactcggc catgttcgtc gagggagcct cgcccttcgg aggatctttc
     1801 cagagccagc tctcgcatac ccagaacgcg gcgttcttca tgagcgccgg cacgcgccgc
     1861 tgcgacagct ttcccgcgcc tgcccacttc tgcttcagcg cccactcgcg atagcggtgc
     1921 gccgctgcca cccacgaccc ctggtacaca ccgaactcca ccggatacgt gtccggccaa
     1981 tccgatcccg cctcgctcaa gttctccgga tgatggatca ccgacgtctg cttccccggc
     2041 tcgatgtcga aattcttcgc ctgcccgtcc gggtcgcgcg tgccgagata gacggaatcc
     2101 cgcccccggc tcagcgcgca gaactgcatc ggccagccgc cggagggatg gcgcccctgc
     2161 acccgctcgt tccacttccg cagaagctgg ccgccttcgc cgaacgtcgg acgcgcgacg
     2221 tcgtactcac ccggggcggg gaagccgttc accagcggga acgcgacacg cgtcagtccc
     2281 cagtagtcgc tgtggttctc gacgaagatc cgccattggg cgataccaga gtccggcggc
     2341 aggtccaccg tcacgcgtac agtcagcgct ttgtcttcga gccaccaacg caggtcgttc
     2401 cactccatca ccgcccgctt cacgccgccg ggcagcgtct cgatgcgagc gaacgtgcac
     2461 ggcttgtagt tgttgttcgc cgcgcgcgtc agcgtgcctc gtccgagcgt cgcctcccac
     2521 agtaggcgct tcccctcgac ggcctggatg tggttccgcc ccgtcttcag gtccaccatt
     2581 gccttcaggc ccatcccgcc ggactcaaac tccagccgca catgattgtt cgccagcacc
     2641 aacccttccg gcgcctgcgc gaacgcaacc gaagcgcaca gtaacagtcc agtcagtcga
     2701 atcatagccg tccgattcta tccggaaatc gctgtcaccg cgcgggtgga gcaggtcatc
     2761 gctttccgtg gcctgctata aaaccgctcc ctcgttgtat aatcagtaaa accggggggc
     2821 aaactgggag tccctttttc cagatcggcg caacgcgcgc acgcgtgcca ttccgcgccg
     2881 ctgtggacgg ctcgccaaga ccatacccgg ccggcatcag cagatctatc cattgggcca
     2941 acaatggggc gaaaaccggc atgaagagag cggcagcggt tcttctgatt tcaatcggcc
     3001 catggctcga gtttcccgcc gtcgtgagcg cgcaagcgga ggcggtcgcc cagggagtcg
     3061 tcgtggcgga ggcagaccac tcgctacttc cggcggcggc gcttgtgctc gaaggcgcgg
     3121 cgcttcccca accattggga gcgacgacag ggaccgacgg gcgttttcgg tttgcacgac
     3181 tgactccggg tgactattcc ttgacagtca cccatgatgg tttccgggag caacggtacc
     3241 ggctgactct ccgacctcat gaggtccagg atcgaaccct cgagatggct ctgcgccccg
     3301 tcgaggaaac cgttcaggtt gtctcggagg cgaatccgtt accctctgtt cactcgccga
     3361 actcgacgtt tctcgcgccg gaccggttgt tactgctgcc tctgacccag cgcaccaact
     3421 tgcccgacgt gatcctccag gcggcgccgg gaatgatccg cggccacgac gacttcgttc
     3481 acatccgtgg ccacgaagtc gctctcagtc ctttcatcaa tggggtctca ttctgggaga
     3541 acccgcacat ggtcttctcg cccggcctgg gcgccgacta catcgaatcc gtgaacgtca
     3601 tgaccggcag cttctccgcc gaatacggga accgttttgg aggcgtgctg gacgtcgtca
     3661 cgaaatccgg ctttacggcg gataatcacg gctcggtcac actcggcgct ggaacggcac
     3721 tgcggcacaa cgcctctctc gagtttggcg gccacacgaa gcgcttcggc tattacctga
     3781 acgccgccgc cttcgagtcc gcgaggtttc tcagcccgcc cgaaccgaat gccatgcatg
     3841 atgccgggcg gggcacgcgc gcctttgcgc agcttgattt caatgccaat gaccgcaacg
     3901 tcctcagact gaccctgatg ggcgacggcg ccgatttcga gcttcccaaa acactgctcg
     3961 atgcccaaat gaggcccgac tttgacaact tccagcgcac gcgctcgcag tcggccatcc
     4021 tgtcgtggga ccacattcgt tccgcggaca cactgattca catggccctc tatcagaggt
     4081 attcccgtgt ccgtgcgttg ccgaatgccg atccctacgg cgcgaaactg gactctacgc
     4141 gcacgctcaa caccaacggg atcaaaaccg actactcgcg cttcttccgc ggccatgcat
     4201 tgaaagctgg tgtggacttg gtcctcctgc ggcccgagga agccttcggc tacttgagcc
     4261 agccctggat caccttcacg catcttccgg acgtcagcca ggatcacatt cacttccgcg
     4321 gccccgacat gggaccgggc atcccgagac cggtcttgtt ccggcaggaa aagaccggtg
     4381 ggcaagctag cgtgtacctt caggacaaaa tcaggctgac gcgcggcctg agcgccgacg
     4441 tcggtttgcg cttcgaccgg cacagcctgg ctatctcgga ttcccacttc agcccccggc
     4501 tgaaccttgg gtatgcgttt cgctccggga cggtgctcta tgggtcttac aaccactact
     4561 ttgtaccgcc gcccgtggaa aacgtcctcg ccagcagcgc tgggctgacg agtctcatct
     4621 ccgagatcgc gactcccttg cctcccctcc gcccggtgat cgagaaccag tttgagttgg
     4681 gcgtggtgca gcccgttgcg cagcggttgc gggtcggggt caccggctac taccgcatca
     4741 gcaacaacgc ggtgcacacg gtcctgttcc cggattccag attctacgcc tacgccaatt
     4801 tcgataaggg gaaggcctac gggatggagg tcaaggcgga ggcgcccatg gtccgcgccc
     4861 tgggactctc tggctacttc aactacgctc tcgggcgcgt ctggttctac aaccccgtga
     4921 ccgccggctt catcacggag gccgagcacc tcagtgaaac gaaccgcttt ctggccccga
     4981 tggatcagac gcacacgctg acttccggta tcaactacca caaccggcgg ctgggacttt
     5041 ggacctcaat ggccttcgaa tacggcagcg gaactcccgg ctcccacggg ggcggcgacc
     5101 acgaagacgc aggggatgag gcggatcacg aacatgcgcc ggtaccggga ggatgcgacg
     5161 tgcgctgtcc cgagcacttc actcagaatc tgtcggtcgg ctgggatgcc gtccgaaaac
     5221 gcgaacggcc gcatctgact ctccagttca acatcgagaa cctgaccaac aacgtttacc
     5281 tgatttcgaa agagagcacc tttgttcagg gccagtactc gatcccgcgc ctcttctcgg
     5341 gctccctcaa gctccgcttc taattcgggc gaccgttccc gccctcttgt cccggcttct
     5401 tcgacgacgc tgttgtctac gacgcggacc gcttccccgg ctacgagcgc tttcagcaag
     5461 gggcgctgct gtccaagggg aacaacctga ggaacgatct cgaaaccatc gacgaatacg
     5521 gctggcgcaa cttcggcgac acctgggcca agaatgaaac cgacaaatcc ggcaacccgc
     5581 acgaaggaca gccggtggtg aaccattaca cctaaaatgc gatcttggct acgggatgct
     5641 ctccagagcc tccgcacgct cggtcgcgag tggtgggatt tcgccgaagc ggcccttcgc
     5701 cacgagagcg acatcgacgt ctaccattcg ttgaacgaga caggctcgcc tgccgtgtac
     5761 gttggcggca agttcaccca cacctcgcag gttcctttct gcctctattt cttccgctcg
     5821 acgacccact cgcaatgcga gtcgccacgc agcatattct tggtgagtct gaacgtaaaa
     5881 tttgggttca aactttcggc ggctccatca ccccatcttt gatgccctac gccgcaggta
     5941 tcaaagtcaa cgccttgctc cttccacctc ttgtgccaag gacactgagt agtctttccc
     6001 acacatctgt ctttggtagc ttcaactacc tcgaacacaa actccggccc cattgacgct
     6061 ttagcaagga gatgtgtaac ccgttcgaca tcttcggcgt tctcagtggc taatccgacg
     6121 gtatcagcaa attcctttgc tccctttcct gcttcatacc agagcgaccc attgaactcc
     6181 tcaaatttct tctgtcctac agcctgcttc aaggctttag aaatggcaat atatgcgccg
     6241 gtcaatcctt tcgttgccat ctcccatctc gttttctcag gaatcgtttc tattttgtca
     6301 ggcattgggc tttctccttc tggatctgtt tcgaaaatgg attcgaccag accggccagc
     6361 ctgcagagtc tctgaccagt tggcttcatt gtactccacc gtccatgctg atgctctctg
     6421 cttccggttg gtcatccgga ggtaatccgc ttcatccgtc acacggaacc gcccggagac
     6481 tagcgtcttg gctttttcac gcggccgact ggcgacgaat ctgccgttgt tgcctagccg
     6541 cctccagctt tcgatccctg gcggcgtgga tctcctactg acgcccggcg agcatgtcct
     6601 tcggcgtgat gtagccgatc gcactgttca ggcggacgtt actgtaatgc tcggcgtagc
     6661 cctccaccag acgctccaag attatctcga tgtccgcctc ggtcatcgac tctcgcagat
     6721 cccagttgac gatgtatcgg ctgcagccgt ccagaatgct gcagacgcct ccttaggatg
     6781 gcaactttct cttccggcgt gtagtgcttc cgctgcttct tcatgcgttc gctccattat
     6841 cttaacgggg gaacgcttct ccatttccag ctgaaccgga accagacccc tggacgcgcg
     6901 acgtcgtacg cgcccggggc tgtaagacaa aaatcagtcg acaatgatga tgatcggcgg
     6961 catttcagcg gccctccttt cgcctccgta actcatgcac tttgcagtga cctgactcca
     7021 ggcccacaac ttctatcggc aagacagggg ggcgctcttt agcgcactgt ggatgatgta
     7081 aaccttccgc cactctcgtg cgtcaatcta aaattagcta tgccgttgaa ctctctatcc
     7141 cgatcggccc tcccggccgt ctaccttctt tttctggctg tatctgcttg cagtggtgga
     7201 acttccttga tggatcggct accgctggag attctcggtg agcggcccgg ccgcaacgag
     7261 gcgggcctcg cgccttcgct cgcctacgcc tcatacatcg gtggtaccgg gactgacgtc
     7321 gtcaccgcag tcgcagcgga tgccgatgga tttctgtggg tcgccggcta taccacgtcg
     7381 acagacctcc cggtcaccgc aacagccgtg gccgctgcgt ccaaaggtgg ccgtgacgcg
     7441 ttcgtgatca aactcgatcc gcgcgcaagc ggcctgtcct cgcttgtcta tgcgacttac
     7501 ctcggcggcg ccggtgacga ggagcccgtc gcgatggtgg tccgtggaaa catggttttc
     7561 ctcgcgggat caacctcatc gacagacttt cccaccgccg gcaatcgtta caaggacacc
     7621 aacagtggca ggcgggacag ctttgtggtg aagatcaaca ccgctgtcgg cggaacggcg
     7681 ggccttttgt actccagcta tgtcggtggc gagaactacg agtatgcgta cggcctggca
     7741 gttgacagca acgaacgcat gtacgtcacg ggttacacta cctccacgac cagtttcccc
     7801 gtcaccgcga atccgctcca ggcatcgaac cgcggcgggt acgaagcgtt cctctatgtc
     7861 atcgacccaa acctcggaac cggcagctcg ctcgtctact ccacctactt cgggggcagc
     7921 agcaccgaca tcgctaccgg catcgcgctc gacgcctcag gcaaggtcta tctcaccggg
     7981 cagaccatgt cggacaactt tccggtgacc ggtgctgcct accagaccgg ctaccacggt
     8041 aaaggcgata tcttcatcac gcggctcgac ctgacaaaat ccggccttgc gggcctcgat
     8101 tactcgacct acttcggggg cggaaacttc gagactgcct acggtatcgc catcgatccg
     8161 gccgggaacg tttgcctcac gggctatacg atgtcgtcag actttccggt cacgcccacc
     8221 gcgtaccagc aaagcttcgg tggcctcgcc gatgtcttcg tcgcgaagtt cgatttcacc
     8281 gctgcgcccg gaaccatgct ctactcgacc tatttgggag gcgtcggcgc cgacatcgcc
     8341 tatgcgctct cggtcggtgc ggcgggccgc atctacgtca ccggatacgc gtactccggc
     8401 ggctttccct ccgctggccc gccgcttcag gagggcagct cgggtataaa cggtgctttc
     8461 ctctcggcag tggacacatc ggcctcggac gcggcgtcgc tgctcttgtc gacgcatttc
     8521 ggggacgggg gcacggtggg ctatggcctg gcggcggatg gcgccggcag tttgcacgtc
     8581 gccggcctca cgatctcgac cgcgctgccg gtcacggaag gcgctctttc caccgtgccg
     8641 cgcggcgcaa cggacggctt cgtgatgaaa gtggacctct cggagggtca gcctctgggc
     8701 gttctctcgc ggaaccagag cgccgccgcg cccaggacgc ccatgagggt catcagcgcg
     8761 ccgaggtaga ccgaccaggg ccggtacttc atttcgatcg tgtgccggcc cgcttcgacc
     8821 acgacgccgc gaatcatccc gttcgcctcg aaaatcttcg ccggcttgcc gtccaccgtc
     8881 gcccgccagc ccgggaaata gctgtctccg acgagcacca tgcccctgca cttcaggtct
     8941 gcttcgaccg taaggctccc gctcgtgcgc ttcagcatcc ggacggattc gtccccattg
     9001 caggtctcaa gctggggcgg ggaactgagc aggaagaccc tcttcctgag gtcgaaattc
     9061 ccgtcgttga tgaagccgat cgcctcgatg ttgttgtggg cctggacggc ttcgtgcaca
     9121 gcccacgccc gcggaaacgc atcggtgttc cagtacagtt tcaaaccgct tttggctgta
     9181 aaaacctcct gctgattcgg cccgggtggt ttccgcgtga ccgaatagtt caccccgaac
     9241 atcttcacaa tacgggcgtt gaattgatcg aagcggacga ggttgaccgg taggctggcc
     9301 aggtagccgc cgtactggtc gatgccatac cagtccccga aattgaacgg gatctcctcg
     9361 gttggcactt caaacctgac gggccacggc tgggttcgta agacctgcgc gagatcggcg
     9421 tttttcgtca gtccctccaa gtacctcaag tgcattggac ggcttttgtc gtcccggttc
     9481 ggccaatagt agccgctgaa gttattgagt tcgatcagca ttagccctgc ggtcagaacg
     9541 cacgccgccg tctgtgaaag cgcgccgcgc cgccatccgt ataggagcat cgcgaagagc
     9601 agggctacaa aggcgttgac cacgaagcgg tcgtcccccg gcggtttctg cgccaggacc
     9661 aacacggcaa ggatcgtgaa gacgacagcg ccgaagaccg acagggcaat ccacatccgg
     9721 cgctgccacg gagcgtgttc cgagctcaga taactatcga ttccataagc aactaggaca
     9781 gcaattccaa agtgaaagat gaacactgcc gtggacacat tccgggcctt ctccacggcc
     9841 ggaatgatgg aatagaggat accgtgaaac acgttgttcc ggccgagcga gaacagcaac
     9901 ccgcccgccg cgatggctcc gaagatcctc accggcaggg acttccagga gagggccact
     9961 cccagtagcg ccagggacag ggccaccagc ccgatcgacg ggtcggtgtg ccggtggtgg
    10021 cccgggatca cgatgccgag cagggaagtc gcgcggagcg aaaaaaagtc gtggacgttg
    10081 tacggtatcg ggtccttcca gccaaccgct tcggatgacc cgacccagcg tttggcgagc
    10141 ttgccgtact cgtacgccgg gagggtctgc agtccgctcg tgaggagaaa aaagaggaca
    10201 aatatggcgc caaaacgcac cattttgacc tcaaaacggc cgcttttgag cacgaaaaac
    10261 agccacaccg cacccgccgc gagggtgatg aagatcggaa tctggtggtg cccgctaagc
    10321 cacgccactc cgaggaaggt ccccgagagg gccgagttcg ccagcggccg ttcgccgcgg
    10381 gtcgcccgca agagaaacag aaacacgagc ggcgcccaga cggcgccgtt gagcatctgc
    10441 ggccagtccg tggttccgat gtaaccgccg aggccgaacg ccagccccgc gatgatcgag
    10501 gccgccctcg agcgtttcaa gtcgcggcaa agccagtagc agaacagcgt ggccatgaag
    10561 tggatcagca cgaaatacca gtgcaggtaa accatccgga tccaaccgtt ccgcaggggt
    10621 agcaggaaca ggatccagtt caggggatag gcgaggccgg gctgtgcctg cccgatgagc
    10681 gactgaccgc cccacaaata aggatcccat agcggaatgc ggccggcgtg aatctcgccg
    10741 gcttgaaact ggaaccacgg gagcacctga tgggcgaggt cggggctgcc ttcgagccac
    10801 gtgtactggt tggtaagaac gatcttccag aagaatccgg tacaaagaag gaataagata
    10861 gcagggccgg tgagatgcac cagccagcgt ggtcgatccc gatcggtgtg gtgcttttcg
    10921 ccgcctccat tggcagtcat cataggttgc atttccgaga aatcagggag tcacccggct
    10981 aagccgccgg ctccggcgaa gacacacaag cgcgaagggg agggcgcaga aggaagcgag
    11041 ggtcaaggct gcgccggcag caacagaatg gggcgcgtgg gagaccacta cacggctctc
    11101 gcccgccggc accgggatcc ctctcagcgc ccgattcact tcgacgatcc cgacgtccct
    11161 gccgtttact tcggcgcgcc aaccgggata gaacagttcg ctgagaacca gtaagcccct
    11221 cccatgagcc tggactctta gctggattgc tctgggttcg tatcgttcaa t
//
LOCUS       DATIFQ010000297         7203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2201493, whole genome shotgun sequence.
ACCESSION   DATIFQ010000297 DATIFQ010000000
VERSION     DATIFQ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2201493"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="hpnH"
                     /locus_tag="VLE22_13970"
     CDS             complement(<1..724)
                     /gene="hpnH"
                     /locus_tag="VLE22_13970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HSB15560.1"
                     /translation="MRFPLSLTAGMAGYIFRNKVHPRPEWQKNNSAQNGSSNPFRIIH
                     HAGTNGGARKPHPMIRKRFPLVLMLEPLHACNLTCTGCGRIREYESTITRKLSLDQCL
                     AASDECGAPVVSICGGEPLLYPEIGPLAAGLLERNRHIYLCTNGMFIKKRLSEFKPDD
                     RFFFNIHLDGLEKTHDICVEREGVFREAIEGLKAAKAVGFKVSTNTTVYKETDMKEIE
                     ALFEYLEPFGVDGHMLAPGYGYS"
     gene            complement(721..2658)
                     /gene="shc"
                     /locus_tag="VLE22_13975"
     CDS             complement(721..2658)
                     /gene="shc"
                     /locus_tag="VLE22_13975"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HSB15561.1"
                     /translation="MSPGVQLGDRLVSCAADAAARTSRYLLGLRNPEGYWLGELTADT
                     TLESDFILLQLWLHPPVDGTWNPPTRRLVDKAVRSILSRQLPDGGFNIYPAGPAEISA
                     TVKAYFSLKLAGVPADGPALSAARERILSLGGLQAANSYVKINLSLFGLYPRENCPSI
                     PPEMILLPGKFLYRMSSWTRAIVVPLSIVHAHNPTRPVPAGFTIEELFLPGASMGFCG
                     GGSCVSWRSLFLAADRALKWWERYGSKRLRRRAIRACERWMVERTRHSDGLAAIYPPM
                     MYAIMAMDLLGYGPDHPDRAEAQRQFDRLMVNDGERFFFQPCFSPVWDTAIAAFALGE
                     AGAVPASKLASTGDWLLSKEVRRKGDWSVKRPDIEPSGWYFEFANEFYPDIDDSAMVL
                     LALHHARGADPRNCRASAERAVRWLLAMQGSDGGWAAFDVDNDWTPLSEVPFADHNAM
                     LDPACPDITGRVIEALCAWGVDPGSPAIRRGVDYLIRTQEQDGSWYGRWGVNYIYGTC
                     FALRGLRAAGESNREAHILRAGEWLRSVQNADGGWGESCASYDNCCFTAARSTASQTA
                     WAILGLLASGDSSSLSVNNGIEYLLESQRSGGYWDEELATGTGFPKVFYLTYHLYRQS
                     FPLLALAEFVRGWRAPSEEGI"
     gene            complement(2655..3647)
                     /locus_tag="VLE22_13980"
     CDS             complement(2655..3647)
                     /locus_tag="VLE22_13980"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HSB15562.1"
                     /translation="MPPSKSGESNAVRRILLLTPRGFCAGVVRAIDVVKIALGLYGAP
                     IYVRKEIVHNRHVVEELRAAGAIFVEELDDVPEGERVIFSAHGVSPAVRSQAVERKLQ
                     VIDATCPLVTKVHLEAVRFARQGHTVVLIGHKDHDEVIGTLGEAPEATILVETVEDVD
                     RLHVPDPAKVTYLTQTTLSLEETRDVIERLRERFPDIQGPPAQDICYATENRQLAVKA
                     VAPMCQLLLVVGSRNSSNSKRLVEVCQKSGVLAHLLDDAAELRAEWFRGVETVAVTAG
                     ASAPEHLVRELVSALQARGFAEVEEVEIKDEDVRFSLPAELAQQAGRLVSIGAL"
     gene            3698..5944
                     /locus_tag="VLE22_13985"
     CDS             3698..5944
                     /locus_tag="VLE22_13985"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSB15563.1"
                     /translation="MRLMLAVALLGTTGVFPAEIARWEAGRAGQGITIEQAEGSRWTL
                     RQEGGSTVAAVEPSRDYFRRAGYRIRTTVSLSDSAWLVLEYLDRGYALVTTAPGAAQS
                     RQRGVARLNTGKFRRAVFHFEKAAFEDTIRVEGIEFLRSASITDLEPTKDPIPDAPPA
                     LSFARHSQRVTTAGADAATPDGLPETLATMRNLLPLAKALGFNGIESYVKWNFIERAP
                     GVFDWSFYDAVVDEVEKHGMQWFPLLIVGSAYTLPEWFYESLDNRGFVCLEHGKRVDI
                     QTIFCGHQERYVRRFLQEFGRHYGPKKALLGVRLGPSGNYGEAQYPARGDWGYKGRPI
                     HTHIGYWAGDACAAPAFQKWLRLKYAGIRDLNRAWGDSYESFEQVKPFLPETAATLRK
                     RIDFADWYMGSMSSWCELWAKWAREAMPGTVIHQSSGGWGPIEIGTDYTHHARTMAEL
                     KGGVRLTNESDNFPQNFAITRMASSAARFYGAALGYEPGGFGSARGVMARLFNCVTNN
                     GEHLFYYHPNLYSNDQAIGRWLEHASLLDRRAKPAIDVAALYPDTAIKLDDEVVRHLW
                     ASAFLSRVEAMRSTADFDYVSEPMVTEGALDRYKVLLFLWGHVTEKPVLDHIAKWVDE
                     GGVLIYPERPRGPLQTVEGDRFVWNRWQRGETGKGRVILFPGDPEPGQPYARFVRDEL
                     RRLGNLRPEVRRAVRLEKPADVYWSVLENGLLLLLNFSDDEATVRLDNGKTLHLAPYT
                     IRTIEKGW"
     gene            6075..6542
                     /locus_tag="VLE22_13990"
     CDS             6075..6542
                     /locus_tag="VLE22_13990"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HSB15564.1"
                     /translation="MKAYATFSPEPGIHLGLAATDRGLSELSFGEVGLDRNDDHPVLQ
                     EAIRQLTEYFLGERQEFSVPLDLHGTPFQLRVWNALLAIPYGETRNYADLARAVGSPK
                     GVRAVGRANGANPVAIIVPCHRVIASDGTLCGYGGGLEIKRKLLELERTGVKQ"
     gene            6547..>7203
                     /locus_tag="VLE22_13995"
     CDS             6547..>7203
                     /locus_tag="VLE22_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15565.1"
                     /translation="MRFVRVLILSFLLLSSLSAQVFSPKVLRKGQVDASDLKSLVAGI
                     YAQAGAQTDREKAEAIWRFFLTDGRFVKPGFWYHIAGWAYEEPVGEVLDPVKLMNSYG
                     FGLCYQIAPLLEAAFEAGGFPDSRVWFLTGHTVTEVFYDGAYHYYDSDMLGYNPVGKG
                     DPKKLPVASVHQIERDGGIMLGKLKSPTEADKSQVDDPWYPADVKEAAMEGLAELFTT
                     T"
BASE COUNT         1407 a         2311 c         2133 g         1352 t
ORIGIN      
        1 ccgagtagcc gtagcccggc gcaagcatgt gcccgtccac tccgaacggc tcaaggtact
       61 cgaagagggc ctcaatctcc ttcatgtcgg tctctttgta gacggtcgtg ttcgtgctga
      121 ctttgaagcc taccgcttta gcagccttca gaccctcgat cgcctcccga aagacaccct
      181 cgcgctccac acagatgtca tgcgtcttct caagcccgtc caggtggatg ttaaagaaga
      241 atcggtcgtc cggcttgaac tccgaaagcc gcttcttgat gaacattcca ttggtgcaga
      301 ggtagatgtg acggttccgc tcgagcaagc cggcggccag aggaccgatc tccgggtaca
      361 gcaggggttc tcccccacag atagagacga ccggcgcccc acattcgtcc gaggccgcaa
      421 ggcactgatc cagcgacagt tttcgagtga tggtcgactc gtactcgcgg atgcgcccgc
      481 atcctgtaca agtgaggtta caggcgtgca acggctcaag catcagcacc agcggaaacc
      541 gcttccggat catggggtgc ggcttgcgcg cgccgccatt cgtcccagca tggtgaatga
      601 tccgaaaggg attggacgat ccgttttgcg cagaattgtt cttctgccac tcaggccgcg
      661 ggtgcacttt gttcctgaag atatagccgg ccatgccggc cgtcagcgac agcggaaacc
      721 tcatatcccc tcctcggacg gcgcacgcca tccccttaca aattccgcca gagccagcaa
      781 cgggaacgac tgccggtaca gatgataggt gaggtaaaac accttcggaa accctgtacc
      841 ggtggccagt tcctcgtccc agtatccgcc gctgcgctgg ctttcaagca ggtactcaat
      901 cccgttattc acactgaggc tgctggagtc accgcttgca agcagcccca ggattgccca
      961 tgccgtctgc gatgcggtac tgcgagcggc ggtgaagcag caattgtcgt atgacgcaca
     1021 actctcgccc cagcctccgt cggcgttctg aaccgaacgc aaccactccc ccgcgcgcag
     1081 gatatgagct tcccggttgc tttcgcccgc cgcgcgcaag ccgcgcagcg cgaagcacgt
     1141 tccgtaaatg tagttcacgc cccagcgccc gtaccagctt ccgtcctgtt cctgcgtacg
     1201 aatcaggtag tcgacgccac gccggatcgc cggtgacccc ggatccacgc cccacgcgca
     1261 cagcgcctca atcacacggc cggttatgtc cgggcatgcc gggtccagca tggcgttatg
     1321 atcggcaaaa ggcacctcgc ttagtggcgt ccagtcatta tccacatcaa acgccgccca
     1381 tccgccatcg cttccctgca tcgccagcag ccaccgcacc gcgcgttcgg ccgaggccct
     1441 gcaattccgc ggatcggcgc cgcgggcatg gtggagcgca agtaatacca tggccgagtc
     1501 gtcgatgtcc gggtagaatt cgttggcgaa ttcgaagtac cagccgctcg gctcgatatc
     1561 cggccgcttc accgaccagt cgcctttgcg cctgacctcc ttcgacagca gccagtcacc
     1621 ggtggatgcg agctttgacg ccggcacggc gcccgcttcc ccaagcgcga acgctgcgat
     1681 ggcggtgtcc cacaccgggg agaagcaagg ctgaaagaag aaccgctcgc cgtcgttcac
     1741 catcagccgg tcgaattggc gctgtgcctc ggcccggtcc ggatggtccg gcccgtagcc
     1801 gagcaggtcc atcgccataa ttgcgtacat catgggcggg tagatcgcgg ccaggccgtc
     1861 ggaatggcgt gtacgctcca ccatccagcg ctcgcacgcc cgaatggctc tgcggcgcag
     1921 ccgcttcgag ccgtatcttt cccaccattt gagcgcacgg tcggcggcca ggaacaggct
     1981 tctccagctc acacacgatc cacccccgca aaatcccatg ctcgcgcccg gaagaaacag
     2041 ttcctcgatc gtgaacccgg caggtaccgg gcgcgtcgga ttgtgcgcgt gcacgatgga
     2101 cagcggcacc actatcgccc gcgtccacga ggacatccgg tagaggaact tgcccggtag
     2161 caaaatcatc tcaggaggga tcgacgggca gttctcccgg ggatacaagc cgaacaggct
     2221 caggttgatt ttgacgtaac tgttcgctgc ctgaagtcca cccagcgaca ggatgcgttc
     2281 gcgcgcggcc gacagcgccg gtccgtccgc gggcacgcct gccaatttaa gcgaaaagta
     2341 tgctttaaca gtcgcgctga tttcggccgg tcccgctggg tagatattaa acccgccatc
     2401 cggcaattgc cggctgagga ttgaccggac tgctttgtcg accaggcgcc gggtcggcgg
     2461 attccacgtt ccatccaccg ggggatgcag ccacaactgg aggaggatga agtccgactc
     2521 cagggtcgta tcagcagtca gttcccccaa ccagtaacct tcaggattgc gcaagccgag
     2581 cagatagcgg gaggtacggg cagctgcgtc cgccgcgcag ctaaccaacc gatcacccag
     2641 ttgaacgcca ggactcatag tgcgccgatg gagacgagtc gcccagcctg ctgggcaagc
     2701 tcggccggaa gagaaaatcg gacgtcttcg tccttgatct cgacctcctc cacctccgcg
     2761 aaacctcgcg cctgaagcgc cgagaccaat tcccgtacca gatgttcggg ggccgacgcg
     2821 ccggcggtaa cagcgactgt ctccacgccg cgaaaccatt cggctctcag ttcggccgcg
     2881 tcgtcgagca agtgggccaa cactccgctc ttctggcaca cctccacgag ccgcttcgaa
     2941 tttgagctgt tccgggatcc caccaccagc aggagctggc acatgggagc cacggccttc
     3001 accgccagtt gccggttctc ggtggcataa cagatgtcct gggccggcgg tccctgaatg
     3061 tcggggaacc gttcccgcaa ccgctcgatt acgtcccggg tctcttcgag gctgagtgtg
     3121 gtctgagtga ggtaggtgac cttggcggga tccggcacgt gcaacctgtc cacatcctca
     3181 acggtttcca ctagaatcgt cgcctccggc gcctcgccaa gcgtaccgat cacctcgtcg
     3241 tggtccttgt ggcctatcag caccaccgtg tgaccttgcc gagcaaaccg gaccgcctcc
     3301 aagtggacct tggtcaccaa agggcatgtg gcgtcgatca cctggagctt tcgctcgaca
     3361 gcttggcttc ggactgccgg agagaccccg tgcgcgctga aaatgacccg ctctccctca
     3421 ggcacatcgt cgagctcctc gacaaagatg gcccccgccg cccgaagttc ttctactacg
     3481 tggcggttgt gaacgatctc cttccgcacg taaattggtg ctccgtaaag gccgagggcg
     3541 attttgacga catcaatggc cctcacgacc cctgcacaga agccccgagg cgttaagagc
     3601 agaattcttc gcaccgcgtt actttcccca gatttgcttg gcggcatact gaagtctcat
     3661 tataacaact ggttggggac accgaggacg gatgagcatg aggttgatgc ttgcagtggc
     3721 gctacttggg accaccggag tctttccggc ggagattgca cggtgggagg ctggacgagc
     3781 aggccaagga attacgatcg aacaggcaga gggctccaga tggaccctgc ggcaggaggg
     3841 aggctcgacc gttgccgccg tcgaaccatc ccgggactat ttccgcagag ccggttacag
     3901 gattcggact accgtttccc tttccgacag cgcgtggttg gttctggaat atctcgacag
     3961 aggctacgcc ctggtcacca ccgccccggg ggcggcccag agccggcagc ggggcgtggc
     4021 tcgcctcaac acggggaagt ttcggcgggc agtctttcac ttcgagaagg ccgctttcga
     4081 ggacacgatc cgggttgaag gcatcgaatt cctccgctcg gcgtccatca cggacttgga
     4141 gccaactaaa gaccccattc cggatgcgcc gcctgcgctg tcgtttgcgc gtcactccca
     4201 acgggtcact actgcgggcg cggacgcggc cacgccggac ggcctccccg aaacactggc
     4261 gacgatgcgc aatctgctac cgctggcaaa ggcgctagga ttcaacggaa ttgaaagcta
     4321 cgtgaagtgg aacttcatcg agcgcgcgcc gggggtgttt gactggagct tctacgacgc
     4381 cgtggtggac gaggttgaaa agcacggaat gcagtggttt ccgctcctga tcgtcggctc
     4441 ggcgtacacc ttaccggagt ggttttatga atcactggac aaccgcggtt tcgtctgcct
     4501 ggaacatggc aagcgcgttg acatccaaac gatcttttgc ggccatcaag agaggtatgt
     4561 gcgccggttt ctccaggaat tcggccgaca ctacggccca aagaaggcgc tcctcggagt
     4621 ccgcctcggc ccaagcggga attacggaga agcccagtac cccgcccgcg gtgactgggg
     4681 ctacaagggc cgaccgatcc acactcacat cggctactgg gcaggcgacg cttgcgccgc
     4741 gccggcgttc cagaagtggc ttcgcttgaa gtacgccggc atccgcgatc tgaaccgtgc
     4801 ctggggagac tcctacgaat ccttcgagca ggtcaagcca ttccttcccg aaacggcggc
     4861 caccctgcga aaacggattg atttcgccga ctggtacatg ggctcgatga gcagttggtg
     4921 tgaactctgg gccaagtggg cgcgtgaggc catgcccggg actgtaatcc accagtcgtc
     4981 gggcggctgg ggaccgatcg aaatcggcac cgactacacg catcacgctc gaaccatggc
     5041 cgaattgaaa ggcggcgttc gtttgacgaa cgagagcgac aacttcccgc agaatttcgc
     5101 gattacccgg atggcctcct ccgcggctcg gttctacggc gccgcgcttg gctatgagcc
     5161 aggggggttc ggcagcgctc gtggcgtgat ggcgcgcctg ttcaactgcg tcaccaacaa
     5221 cggcgaacac ctgttttatt accacccaaa cctctactcg aacgaccagg ccatcggacg
     5281 ctggctggaa cacgcttctc tgctcgaccg gcgggcgaaa ccggctatcg acgtagcggc
     5341 attgtacccc gacacagcca tcaaactcga cgacgaggtc gtgcgtcacc tctgggcctc
     5401 cgcgttcctt tcccgggtcg aggcgatgcg gtcgacggcc gatttcgatt acgtcagcga
     5461 gccgatggtt accgaagggg cgctggaccg ctacaaagtg ctgctgtttc tgtggggtca
     5521 cgtcaccgaa aagccggtgc tggatcacat cgccaagtgg gtcgatgagg gcggcgtatt
     5581 gatttacccg gagagacccc gcggccccct ccaaactgtc gagggcgacc ggttcgtctg
     5641 gaatcgctgg cagcgtggag aaaccggcaa gggacgcgta atcctcttcc ccggagaccc
     5701 ggagcccggc cagccttatg cccgcttcgt gcgcgatgaa ttgcgccggc tcggcaacct
     5761 ccggccggag gtccgccgcg cggtgcgctt ggaaaagccg gctgacgtct actggagcgt
     5821 cctcgaaaac gggctgctgc tgttactgaa cttcagcgac gatgaggcga ccgtacggtt
     5881 ggacaacggc aagacgctcc acttagctcc atatacgatt cggacgatag agaagggctg
     5941 gtgaccggac agacaagtcg agttgactgg ccgtgatgcg atggaacgtc gtcgtcggcc
     6001 gcgccgtggg atgcgggcat gcacaccgcg ttgcagtcgg cgcccgcgaa ggcccctgct
     6061 accaaaggtg aactatgaaa gcatacgcga ccttctcccc ggaacctggt atccaccttg
     6121 ggctagccgc gactgatcgc ggcctctctg agctttcctt cggcgaggtc ggactggatc
     6181 gcaacgacga tcaccccgtc ttgcaagagg ccatccgcca gcttactgag tatttcctcg
     6241 gagaacgcca agagttcagc gtgccgcttg acctccacgg caccccgttc cagttgcgtg
     6301 tgtggaatgc tctgcttgcg atcccctacg gcgagacgcg gaactatgcc gacctcgctc
     6361 gcgcggtcgg ctccccgaaa ggagtccgtg cagtcggccg ggcaaacggc gcgaatccgg
     6421 tcgcgattat cgtgccctgt caccgcgtaa tcgcctccga cggcacgctc tgcggctacg
     6481 gcggtgggct ggagatcaag cgaaagctgc tggagttgga gcgaaccggc gtgaaacaat
     6541 gagggcatgc gtttcgtgcg tgttctgatt ctctcattcc tcctgctttc ctccctaagc
     6601 gcccaagtgt tctcaccgaa agtacttcga aagggccagg tggatgcgag cgacctcaaa
     6661 tcccttgtcg ccggcatcta cgcacaggcc ggagcgcaga ccgatcgcga gaaagcggag
     6721 gccatctggc gcttcttcct caccgacggc cgcttcgtga agcccggctt ctggtaccac
     6781 atcgccgggt gggcatacga agagcccgtg ggcgaggtgc tcgacccggt gaagctgatg
     6841 aacagctacg gctttgggct ttgctaccag atcgcgccgc tgcttgaagc ggcctttgaa
     6901 gccggcggtt ttcccgactc ccgcgtctgg ttccttaccg gccacaccgt caccgaggtc
     6961 ttctacgacg gcgcttatca ctactacgac tccgacatgc tcggctacaa ccccgtcggc
     7021 aaaggtgacc cgaaaaagct gcccgtcgcc tcggttcacc agatcgagcg cgacggcgga
     7081 atcatgcttg gcaagctgaa gtcacccacc gaggccgaca agtcgcaagt agacgatccc
     7141 tggtacccgg cagacgtcaa agaagccgcc atggaaggtc tcgctgaact ttttacgaca
     7201 act
//
LOCUS       DATIFQ010000298         9354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_95413, whole genome shotgun sequence.
ACCESSION   DATIFQ010000298 DATIFQ010000000
VERSION     DATIFQ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95413"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VLE22_14000"
     CDS             <1..629
                     /locus_tag="VLE22_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15566.1"
                     /translation="MTLSPPAIPRMPDLQVTPHIGGKYAAFLTEARAQMPENEALKLE
                     LHAQFGLADRNRYNLEVFLSIAELVGHHWRLLTEMAGAEQDLVRAYAAAQKSQHRRAV
                     GQLVAAYNRIDRIERERERTLHELQQVWEMSRYPRGQSVGGRDFVDVNDDTKDYWAQR
                     RPDLRYLTAPEESIGLKEWQKSLRGVMEAYAKQHNVPVRGLAEARLEE"
     gene            631..2031
                     /locus_tag="VLE22_14005"
     CDS             631..2031
                     /locus_tag="VLE22_14005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB15567.1"
                     /translation="MRLLLALCIVTVNLYAQAQGNTADIETLLRQHRAAPGDWRVCNQ
                     IALAYTQLGQFDNARDYYQRVLKLNPTFLPARKNLGTVLWFLGKKQESEREFQRVVKS
                     APKDPVPHLYLGLAQFERKNFVSARSHFGSAGDLAFRNPEVLPAVLETYLATKDTDVP
                     AKLLELLKLAERPDPQLVIRVASVLVTYGKYAEAIESLEKIAGSSDAKPEVYLLLAEV
                     YDKNGEPQKAYEAYARSIRVAPRSEESYVGLAAFASAHKNDEFALRIVTQGLEHVPGS
                     PRLLLLKGVYAALLGKAEEARQSLLEAGKADPMSSMPHLALGVSQLERGNYDEAAGQF
                     RRAAEISPDDFRAEYLYATALRRSGNPAHREKIIASFERAARLKPDDPRPHASLGQAY
                     FEGGRLKEAAAELEKALALDPENTTALYQLGLLYRDLGRSEDSGKMLRRFRESKTRQQ
                     EEKEGELVQMLKVVKD"
     gene            complement(2035..3594)
                     /locus_tag="VLE22_14010"
     CDS             complement(2035..3594)
                     /locus_tag="VLE22_14010"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB15568.1"
                     /translation="MAALPALLALVASLLQASAPSREERQWLLEAQQRFQNRQWDEAR
                     NAALKALEANPKSGDAETVLGLVETMRSRFQEAERHFRNAAALQPANPRVHAYLGSTY
                     LQQQRLPEAGKAFERVLQLDPGNSAAHYNLGLIGLAGGNAAAALAHFDHVRNADAKDI
                     PALIGALECQLLLKRAAEARQSADRLQKLLDARDPRLFQTATLLAVHGEYPSAIPILE
                     RIREVLPDSADVHYNLALAYHRSGQHDKAADSLKPLLATRGRAETYSLLASIEESRNH
                     PQEALRAFRRAAELEPGSEEFRFDHATALLRYEYPEAAGVEFERALRDFPNSWKIRLG
                     LGGHYYLQGKYEDASRILLDAVRIEPNAPLAYHLLGLSYESAGSRQPEILRVLERYVD
                     GKPADAWAYYHYGTILYTQSQAQRQDPPERAKMSLRRALELNPDFPEALLQLGTMAES
                     DEESVPFYERAIKLKPDLALAHYRLGLAYQRMGEGEKAKTELALFRKLRAENEEAEKR
                     RVLETLTRQSK"
     gene            complement(3675..5465)
                     /locus_tag="VLE22_14015"
     CDS             complement(3675..5465)
                     /locus_tag="VLE22_14015"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HSB15569.1"
                     /translation="MKTAGVLCPGALLLCYPLFAAGQWFERPVPEWETKFQVWLADPH
                     KHVYPASFGRDLDNRRIEVRACRNEYVVIQLAARSPEPVSALSVHPSELRSPAGDRLG
                     AELIRVRYPGLMPVDENAQYTPDPLWEAATVALLPYQSQGIWIDLRAPATAKPGRYEG
                     TIEVRGGPTPETFRLALDVLPVALPDPTRFRCYLNILVDPSSVARFNNLPLWGEEHWR
                     QLDRYVANLAAHGQKSITAFIVDDPWAGDTGFAVRNLIEWKHSGEWKAGAAGGWTFDF
                     TPFDRFVAMCLRNGIDDHIECWSPLVQPGSDHSVVIYTDTRTGERRKLRLPAGSAEYE
                     AVWGEFARNFQEHLRAKGWLGKAYLAFDEIKTEVLDRTLPFFQKTTPDLKLMISGGDE
                     KGKHMAYSREMAFYYGYFSPGAPAELPDIPARRKAGKRTLLYTAVSPLYPNTFIFSDP
                     LESRYLGWIVWKWDFDGYIRWAWNFWPEHLWDQPLYKWPSGDMFFVYPGKEGPVDSIR
                     WEMLREGLEDYECLWIAKQGLEKLRQSGRNPEVVTRGDKAISRAVDLATQQFDRDRAP
                     KDVVPSRIHEARKIVDDLLLELSRLGVAGG"
     gene            complement(5546..8860)
                     /locus_tag="VLE22_14020"
     CDS             complement(5546..8860)
                     /locus_tag="VLE22_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15570.1"
                     /translation="MPKQKSYAPPNRRTPLGILTCLVALCLFVAAIPAAAQIATGTIS
                     GYVKDPTGAAIPTATVTAKLVLQQAVRTTDTNTEGFYTMQALPPGTYEVTFESKGFQR
                     QTQTNVQLTVNQNLRLDAALAVGAVETQVTVEATAPLVDTTSHTLSGLVDDRRVVDLP
                     LNGRNVIALARILPGVLNVAAPQQMSDARGGPEMNVNGGRSNMNLFTFNGGYFNNPSR
                     NTGMNYPPPDAVQEVRILTHNFAAEYGRNPGSQVTVVSRSGTNEFHGSAWEFLRNDNL
                     NARNFFSSRVPTLIQNQFGVAGGGPVVKDKLFFFGSYQGLRDRREAQTVEAFVPSAAQ
                     RNGDFTGLDFELTNPANPITGAPFTDSAGQPCVQGNKILSGCISPVAKNLLEFVPESP
                     SGSVSSLAASPRDGDMWMVRGDWNQSNNHRIYGSYFSDHNKRASPFAAGGNIPNYISE
                     SFVQDTRHLTINDTYTISPTLLNQATFTFLDTPSDQVQTRTIDPKDLGINMPQYLPTG
                     AVSVDVGGGFVLGSGFTTRFYSRNWQFREALTWIKGKHNFKFGYEVLRLQFRQVFIGS
                     PGFSFSGSRTGDPVADFMVGAFDSLSLNFGIRDTDARTWAHAAFFQDEFKVTPRFTLT
                     LGIRYEPFLPWIEKDDRINTVAPGIKSKVVPDAPVGVLFPGDVPRGLADNDLNNFAPR
                     IGFAWDIFGNGKTSLRGGYGIFYESVNGDSLAQENPPFAGFSSVYSGRVEDPYGSVGL
                     TPPPAQTTSKFGCVEIPTYPGYDCPLFPLPIGGVFTQRNVVAPYIQSFNLSVQHQLTP
                     TLMVETAYAGKIGTKIEALRTYNPARFINSPVDGSPPSDQNINDRVIFEPGILGPQGF
                     LLGNDFRSWYHSFQAQVTKRFSSGLSILGAYTLSKSIDSSSTDNLGATVANPFNLRDE
                     RGRSDWDRRHAFVASWLWSPPIKFASPVANTLLGGWTLTGIHTLQSGGPLTFLMGDDV
                     ALDGTFGDQHAQLKPNVTVDNIVLSHSDRAGMVQRFFNTDAFVPTNDVPRGIYGNAGR
                     GLISGPGMSSSDLSVLKDFLFREPWRLQFRSEFFNAFNQVNFTSVTTTVNSSAFGRIR
                     RASDGRVIQFGLKLIW"
     gene            9062..>9354
                     /locus_tag="VLE22_14025"
     CDS             9062..>9354
                     /locus_tag="VLE22_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15571.1"
                     /translation="MAEFSGEPQSMKLDRRSFLLSSAVAAAKLDAKAQERQEIRAAFV
                     GAGNRGSSLLSEALEQHNIKVVAVCDTNPEARDKAATAASRHQPRSYSNWKQVV"
BASE COUNT         1864 a         2646 c         3041 g         1803 t
ORIGIN      
        1 atatgacgct ttcaccaccg gcgattccgc gaatgccgga ccttcaagtg acaccgcaca
       61 tcggcggcaa gtatgctgcc tttctcacgg aggcccgcgc gcagatgccc gagaacgagg
      121 cgttgaagct ggaacttcac gctcagttcg gcctggccga caggaaccgc tacaacctgg
      181 aggtattcct ttcgatcgcg gagttggttg ggcatcactg gcggctcctg actgaaatgg
      241 ctggggccga gcaagacctt gtgcgtgcgt acgccgccgc gcagaagagc cagcaccgga
      301 gggcagtggg ccagttagtg gccgcctaca accggatcga ccggatcgaa agggaacgtg
      361 agcggaccct gcacgagttg cagcaagtct gggagatgag ccgatatccg cgcggccaga
      421 gcgtaggcgg acgagacttc gtcgacgtca acgacgatac aaaggactac tgggcgcagc
      481 ggcggccgga cttgcgctac ctgaccgctc ccgaagagag catcggcttg aaggaatggc
      541 agaagagcct ccgtggagtc atggaggcgt atgcgaaaca gcacaatgtt ccagtgaggg
      601 gactggctga ggcgcggctg gaggagtgag atgcgcctcc tgctggctct ctgcatcgtc
      661 acggtcaatc tgtacgccca ggcccagggg aacacggcgg acatcgagac cttactgcgg
      721 cagcaccgag ccgcaccagg cgactggcgc gtttgtaacc agattgcact cgcctatacc
      781 cagctcgggc agtttgacaa tgcccgcgac tactaccagc gcgtgctgaa actgaacccg
      841 acgttcctgc cggcgcgaaa gaacctgggg actgtactct ggtttttagg caaaaaacag
      901 gagtcggagc gagagttcca gagggtagtg aagtccgcac cgaaggaccc ggtgccgcac
      961 ctttaccttg ggctggcaca gttcgagcgc aagaacttcg ttagcgcccg atctcacttc
     1021 ggtagcgccg gcgacctggc gttccgtaat ccggaggtcc tgcccgctgt gctggagacg
     1081 tacctggcaa cgaaggatac cgacgtgccg gccaagctgc tcgaacttct gaaactggcc
     1141 gagcggcccg atccgcagct tgtgattcgg gtcgcttcgg tgctcgtgac atacggtaag
     1201 tatgcggaag cgattgagag cctggagaag atcgccggct caagtgacgc gaagccggaa
     1261 gtctacctgc tgctggccga agtatacgac aaaaatgggg aaccgcagaa agcctacgaa
     1321 gcctatgcga ggtcgatccg cgtggcgccg cgttcggagg aaagctacgt ggggctggct
     1381 gcctttgcct cggctcacaa gaacgatgaa ttcgcgctgc ggatcgtcac gcaaggttta
     1441 gagcacgtgc cgggttcacc gcggcttctg ctgttgaagg gtgtgtatgc ggcgcttctg
     1501 gggaaggcgg aagaggccag gcagagcctg ctcgaggcgg gaaaagcaga cccgatgtcg
     1561 agcatgccgc acctcgctct cggcgtctca cagcttgaga gaggcaacta tgacgaggcg
     1621 gccgggcagt tccggcgcgc cgctgagatt tctccggacg actttcgggc cgagtatctc
     1681 tatgcaacgg ccttgcgccg gtcaggtaat cccgcgcatc gggaaaagat catagcctcc
     1741 ttcgagagag ctgcgcggct caagcctgac gatccgcggc cccacgcttc gctgggccag
     1801 gcctattttg agggcggccg gctgaaggag gccgccgcgg aactggaaaa ggcgctcgcg
     1861 ctggaccccg agaacacgac ggcgctatac caactcggtc tgctttaccg ggatctgggc
     1921 cggtccgagg actccggaaa aatgctgcgg cggtttcgtg aatcgaagac gcgacagcag
     1981 gaggagaagg agggggaact ggtgcagatg ctgaaagtcg tgaaggacta gaagttactt
     2041 gctctgccgg gttagcgttt cgagaactct tctcttttca gcctcctcgt tctccgcccg
     2101 cagctttcgg aacagggcga gctctgtttt cgccttctcc ccctccccca ttcgctgata
     2161 ggcaagcccg agccggtagt gggcgagcgc gaggtcaggt ttcagcttga tggcccgttc
     2221 gtagaagggg acgctctctt catcggactc tgccatcgtt ccgagttgaa gcaacgcctc
     2281 ggggaaatcg gggttcaact ccagagcccg gcggaggctc attttcgccc gttctggggg
     2341 atcctgccgc tgggcctgag actgggtgta cagaatggtc ccgtaatgat agtaggccca
     2401 ggcgtccgcg ggctttccgt cgacgtagcg ttcgagcacc cggaggattt cgggttgccg
     2461 cgagcccgcc gactcgtagc tcaggccgag cagatgatat gcgagcggcg cgttgggttc
     2521 gatccgcaca gcgtcgagaa ggatgcggga ggcgtcttcg tacttgccct ggaggtagta
     2581 gtggccgccg agcccgagtc gtatcttcca ggagttcggg aagtcgcgca aggctcgctc
     2641 gaactccacg cccgcggctt ccggatactc gtagcgcaga agagcggtgg cgtgatcgaa
     2701 gcggaattct tcgcttcccg gctccaactc ggccgctcgc cggaaggccc gcagggcttc
     2761 ctgagggtgg tttcggctct cttcaatgga ggcgaggaga ctgtaggttt cagcccggcc
     2821 acgggtcgcg agaaggggtt tcaagctgtc agcggcctta tcgtgttgtc cggaacggtg
     2881 gtaagccagc gccaggttgt agtggacgtc cgcagaatcc ggcaggacct cccgtatccg
     2941 ttcaaggatc gggatcgcgg aggggtattc gccgtggacc gcaagcaggg tggccgtctg
     3001 gaagaggcgc gggtcgcggg cgtcgagtag tttctgcaac cgatccgccg actgcctggc
     3061 ttccgccgcc cgcttcaaca ggagctggca ttcgagagcg ccgatcaggg cgggaatgtc
     3121 cttcgcgtcc gcgttccgga cgtgatcgaa atgggcgaga gcggccgcgg cgttccctcc
     3181 agccaagccg atcaagccga gattgtagtg cgcggcgcta ttaccgggat caagttggag
     3241 gacccgctcg aacgctttgc cggcttcggg gaggcgctgc tgctggaggt aggtggagcc
     3301 caggtaggcg tggactcgcg ggttggcagg ctggagcgcg gcggcgttcc ggaagtgccg
     3361 ttcggcctct tgaaagcggg accgcatcgt ttcgacgagt ccgaggacgg tttccgcgtc
     3421 tcccgacttc ggatttgctt caagagcctt cagcgccgca ttccgggcct catcccactg
     3481 gcggttctgg aaacgctgct gtgcctccag gagccactgc cgttcctcgc gagaaggtgc
     3541 agacgcctgt agtagcgacg caaccagcgc gagcagtgcg ggtagggcgg ccatccactc
     3601 aaatataact ttagcgctca gcaatcagcg gtcagcttaa agctgatggc tgaatgctga
     3661 aagctagcct tgcttcagcc accggcgaca ccgaggcggg aaagctccag aagcaggtcg
     3721 tcgacgatct ttctggcctc gtggattcgt gaagggacga cgtccttagg cgcccggtcg
     3781 cggtcgaact gctgggtcgc gaggtcaacg gcccgtgaga tcgctttgtc gccgcgagtg
     3841 acaacctccg gattgcggcc ggactgacgg agtttctcga ggccttgctt ggcaatccag
     3901 aggcactcgt agtcttcgag cccttcacgg agcatctccc agcggatact gtccaccggt
     3961 ccttctttgc cgggatagac gaaaaacatg tcgccggagg gccacttgta gagaggctgg
     4021 tcccagagat gttccggcca aaagttccag gcccagcgga tgtacccgtc gaaatcccat
     4081 ttccaaacga tccagcccag gtagcgggac tcgagcggat cggaaaaaat gaaggtgttg
     4141 ggatagagtg ggctcaccgc ggtgtacagc agcgtacgtt tgcctgcttt gcgccgggct
     4201 gggatgtcgg gcaactctgc gggtgcgccg gggctgaaat agccgtagta gaaggccatc
     4261 tcccgcgagt aggccatgtg ttttcccttc tcatcgccac cgctgatcat gagcttgaga
     4321 tccggcgtcg ttttctggaa gaacggaaga gtccgatcga ggacctcggt cttgatctca
     4381 tcgaaggcga ggtaagcttt gccgagccaa cctttggcgc gcagatgttc ctggaagttc
     4441 cgggcgaact caccccaaac ggcttcgtat tcggcggagc cggcgggcag ccggagtttc
     4501 cttcgctccc cggtgcgggt gtcggtgtag atgacgacgg agtggtccga gccgggctgg
     4561 acaaggggcg accagcactc gatgtggtcg tcgatgccgt tgcggaggca catggcaacg
     4621 aaacggtcga acggtgtgaa gtcaaacgtc catccgccgg cggcgccggc tttccattca
     4681 ccggagtgtt tccactcgat caggttgcgc acggcgaaac cggtgtctcc ggcccaggga
     4741 tcgtccacaa tgaacgcggt gatcgatttc tgaccgtgag cggccagatt ggcgacgtat
     4801 cgatcgagtt gccgccaatg ttcttcaccc cagagcggca gattgttgaa gcgggccacc
     4861 gaggacggat ccaccagaat attgagataa caccggaagc gggtcggatc aggcagcgcg
     4921 accggaagaa cgtcgagcgc aagacgaaat gtctcggggg tgggtccgcc gcggacctca
     4981 atggtgccct cgtagcgccc gggttttgcg gtggccggcg ctctgagatc gatccagatg
     5041 ccctggctct ggtatgggag cagggcaaca gtcgcggcct cccagagcgg atcgggggtg
     5101 tactgagcgt tttcgtcgac aggcatcaga cccggatagc ggacgcggat cagctccgcc
     5161 ccgagccggt ctccggcggg cgaccgcaat tcggaaggat ggacagagag tgccgaaaca
     5221 ggctcagggc tacgcgcagc aagctgaatg accacgtact cgttccggca cgcccgcact
     5281 tctatacgac ggttgtcgag atcccggccg aagctcgccg gatagacatg cttgtgcgga
     5341 tcagcgagcc agacttggaa tttcgtttcc cactccggga ctgggcgctc aaaccactgg
     5401 cccgcggcga agaggggata gcacagtagt agtgcgcccg ggcacagcac acccgcggtt
     5461 ttcatcgagg agacctccga agtcaggaga aacagaaatg cgccggaaag gtctgggagg
     5521 acctttccgg cgcatgagga gagctttacc agatcagctt caggccgaac tgtatgacgc
     5581 ggccgtctga cgcgcgccga atgcggccga acgcagacga gttgaccgtg gtggtgacgc
     5641 ttgtgaagtt cacctggttg aaggcgttga agaactcgga gcggaactgc aatcgccagg
     5701 gctctctaaa gaggaagtcc ttcaacacgc tcaggtcact gctactcatc cctgggccgc
     5761 tgatcagacc ccggccggcg ttgccataga tgccccgcgg cacatcgttg gtcgggacaa
     5821 aggcatcggt attgaagaac cgctgtacca tgccggcacg gtcagagtga ctcagcacga
     5881 tgttgtcgac agtgacgttt ggctttaatt gtgcgtgctg gtcgccgaaa gtgccgtcga
     5941 gcgccacgtc gtcccccatc aggaacgtca acggccctcc gctctgcaag gtgtggattc
     6001 cagtcagcgt ccagccgcct agcagggtat tggcgactgg gctggcgaac ttaatgggcg
     6061 gagaccagag ccacgaggcg acgaaagcgt gcctgcggtc ccagtcggac cggccacgtt
     6121 cgtcgcgcag attgaatgga ttagcgacgg tggcgccaag gttatcggtg gaactcgagt
     6181 cgatcgactt cgacagcgtg tacgcgccga gaatggagag cccgctggag aaccgcttgg
     6241 tgacctgcgc ctggaagctg tggtaccaac tccggaagtc gtttccgagc aggaaccctt
     6301 gagggcccag aataccgggc tcgaaaatca cgcggtcatt gatgttctgg tccgacggcg
     6361 gcgatccgtc caccggcgag ttgatgaagc gcgccgggtt gtacgtgcga agcgcttcga
     6421 tcttggtgcc gatcttgccg gcatacgcgg tctccaccat cagtgtcgga gtcaattgat
     6481 gctggacgga caagttgaag gactgaatgt acggtgcgac cacgttgcgc tgtgtgaaca
     6541 ctccaccgat gggcaatggg aacaacgggc aatcgtagcc ggggtaagtc gggatctcga
     6601 cgcagccgaa cttcgatgtg gtttgcgcgg gcggaggcgt cagcccgacc gacccatacg
     6661 ggtcttcgac tcgcccgcta tacacgctgg agaagcccgc gaaaggcgga ttctcctggg
     6721 cgagagagtc cccattgacg ctctcataaa agatcccgta acctccccgg aggcttgtct
     6781 ttccgttgcc gaagatatcc caggcaaagc cgattcgcgg cgcgaagttg ttcaggtcgt
     6841 tgtcggcgag accccgcggt acatcgcccg gaaacaggac cccgacgggt gcgtccggaa
     6901 caaccttcga ctttatccct ggagcgacgg tgttgatgcg gtcgtccttc tcgatccacg
     6961 ggaggaacgg ctcgtagcga atcccgaggg tcagagtgaa gcgaggcgtg accttgaact
     7021 cgtcctggaa gaaggcggcg tgcgcccaag tccgcgcgtc cgtgtcgcgg atgccaaagt
     7081 tcagcgaaag gctgtcgaac gccccgacca tgaagtcggc cactgggtcg ccggtgcgcg
     7141 atccggaaaa cgagaagccc ggggaaccga tgaagacctg gcggaattga agccggagca
     7201 cctcataacc gaacttgaag ttgtgcttac ccttgatcca ggtcaatgct tcccggaact
     7261 gccagtttcg gctgtagaac cgggttgtga acccagagcc gagaacgaat ccgccaccca
     7321 catcgaccga aactgcgccc gtcggtaggt actgcggcat gttgatgccg aggtccttcg
     7381 gatcgatggt ccgcgtttgc acctgatcgg acggtgtgtc gaggaacgtg aacgtcgcct
     7441 ggttcaacag ggtcgggcta atcgtgtaag tgtcgttgat tgtcaggtgc cgggtgtcct
     7501 gtacaaagct ttccgagata tagttgggaa tgttgccacc tgcggcgaac gggctcgccc
     7561 gtttgttgtg gtcggaaaag tagctgccgt agatgcggtg gttgttgctc tgattccagt
     7621 cgcctcgaac catccacatg tcgccatcgc gtggactggc agcgagtgaa ctaaccgacc
     7681 ccgacggcga ttccggtacg aattcaagca ggttcttcgc caccgggctg atacagccgg
     7741 atagaatctt gttgccctgc acacaaggct ggccggctga atcggtgaac ggagcaccgg
     7801 tgatcgggtt ggccgggttc gtgagctcga agtcgagacc agtgaagtcg ccgttacgct
     7861 gagcagcgct cgggacaaac gcttcaaccg tctgcgcttc ccgccggtcc ctgagaccct
     7921 ggtacgagcc aaagaagaac agcttgtctt taacgacggg ccctccgccc gcgacgccaa
     7981 actgattttg aatcagcgtg ggcacgcgcg acgagaagaa gttgcgcgcg ttgaggttat
     8041 cgttgcggag gaactcccac gcggagccgt ggaattcatt cgtgccggag cgggagacga
     8101 cggtcacctg cgaacccggg ttgcgcccgt actccgccgc gaagttgtgc gtgagaatgc
     8161 ggacttcttg aacggcgtcc ggcggcgggt agttcatacc ggtgttgcgc gaggggttat
     8221 tgaaatagcc gccgttgaag gtgaacaagt tcatgttgga gcggccgccg ttgacgttca
     8281 tctccggtcc gccgcgcgcg tcgctcattt gctggggagc ggctacgttc aggacgcccg
     8341 gcaggatgcg ggcgagagcg atcacgttgc ggccgttgag cgggaggtca acgacccggc
     8401 gatcgtccac gaggcctgag agggtgtgcg aggtggtgtc gaccagtggc gccgttgctt
     8461 cgactgtcac ctgggtctcc actgcgccaa ccgcgagagc ggcgtcaagg cgcaagttct
     8521 ggttcaccgt caactgaaca ttcgtctggg tctggcgctg gaagcccttg ctctcgaacg
     8581 taacttcata agtcccgggc ggaagggcct gcatcgtgta gaagccttcg gtgttggtgt
     8641 cggtcgtccg aacagcctgc tgcaagacca actttgctgt gacagttgca gtcgggatcg
     8701 cggccccagt cgggtccttc acatacccgc ttatggtgcc ggtggcgatc tgggcagcgg
     8761 caggaatggc agcaacgaat agacacaacg ccaccaagca ggtcagaatc ccgagtggtg
     8821 ttcgcctgtt gggaggcgca taactcttct gttttggcat tcttcggacc cccgatgtgc
     8881 ggatatgttt agtcctcgaa gtctagctcc gctaacaggt ttatactacc ctattcacga
     8941 tggagtcaag gcgatacata ttaattcttt cagtaaagta ttcgacttgt taattttaca
     9001 taagttacag gaatatgact gatccttaaa gttcttccaa cgcagtgacg gaagaggtac
     9061 aatggccgag ttttcaggag aaccacaatc catgaaactc gacaggcgaa gttttctgct
     9121 ctcgtccgct gttgcggccg ccaagctgga tgcaaaggcg caggaaagac aagaaattcg
     9181 tgcggctttc gtcggcgctg gcaatcgtgg gagcagcctc ctcagcgagg cactcgaaca
     9241 gcacaacatt aaggtggtgg ctgtttgtga cacgaatccg gaggcccgag ataaggcagc
     9301 tacggcggct tcccgccatc agccgcgttc gtacagcaat tggaagcagg tcgt
//
LOCUS       DATIFQ010000299         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2394573, whole genome shotgun sequence.
ACCESSION   DATIFQ010000299 DATIFQ010000000
VERSION     DATIFQ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2394573"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VLE22_14030"
     CDS             complement(<1..1246)
                     /locus_tag="VLE22_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB15572.1"
                     /translation="MGGRSARAIRFGVFEVDLQAGELRKNGLKIRIQEQPLQILAVLI
                     ERPGDVVTREDLRQRLWPTDTFVDFDHSLNTAVNKLREALGDSADSPRYVETLPRRGY
                     RFVAPLELLGEDLAEPAPGQPEPPPTRTRRLLPWERIAFIAGLAVAVGIIVTMWLSRR
                     GTVSEAPVQRFTFAPEGGATTPVISPDGKHIAYLSGSGDDARAMVQDLDRFAPREIPG
                     SEGAKEYIFWSQDSGHIGFAAGGRLWKAPTPGGDPAAICQLPGEYLGGAWSPDGLSIV
                     FSVSHKGLYEVPARGGTPKLIVPVDMNRWGDHFELPWFLPASGGGRVFLYTAQTRERT
                     HVTVLHSLGSGEQRIVAPNGLGVFSPTGHLVYWNRSLLWALPFSSNTKKVTGDAFPIA
                     GTAVFTYASVSLDGTLVYLESGD"
     gene            1487..2116
                     /locus_tag="VLE22_14035"
     CDS             1487..2116
                     /locus_tag="VLE22_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15573.1"
                     /translation="MNWVFRNCWTVGFLLATNAPSAEQPVHPDITVWIYNYAGIDGEA
                     LARAKEAAGRILSRAGTEAEWVDCPVMASEVDKYPDCVRLPQFLGLVLRLRPGSAPKS
                     LVGRSTVFGYALIPEGGGFGTYADVYAGGADLLANGGESLRGVMLGHLMAHELGHLLL
                     GTSKHSAAGIMRSPWSGLETERAAQGRLLFNRAEAESMRTNLAARLRHQ"
     gene            complement(2150..>2350)
                     /locus_tag="VLE22_14040"
     CDS             complement(2150..>2350)
                     /locus_tag="VLE22_14040"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HSB15574.1"
                     /translation="HAGTRAGPDGVMTAGGRVLGVTASGETLAAAIEKTYSAVAKIRF
                     DGMQYRKDIGAKGLKRWVSPSE"
BASE COUNT          443 a          748 c          699 g          460 t
ORIGIN      
        1 gatctccgga ttccaagtac accagcgtgc catccagcga aacactggca tatgtgaaca
       61 ccgccgtccc ggcgatcggg aaggcgtcac cagtcacctt ctttgtgttc gatgaaaacg
      121 gcaaagccca gagcaaggac ctgttccaat acaccaagtg gcctgtcggt gagaagacgc
      181 cgagcccatt cggagccaca attctctgtt ctcccgatcc gagagaatgg agcacggtta
      241 cgtgtgtcct ctcgcgggtt tgcgcggtat acaggaaaac gcggcctcca ccgctcgctg
      301 gcagaaacca agggagttca aagtggtctc cccaccggtt catgtccacg gggacgatta
      361 gcttgggcgt gccccctcgg gccgggactt cgtaaaggcc tttgtggctc accgagaaga
      421 cgatggacag cccatccggg ctccaggcgc cgccaaggta ttcaccgggg agctgacaaa
      481 tggcagccgg gtcgccgcca ggtgtcgggg ccttccatag gcggcctccc gcggcgaagc
      541 cgatgtgacc actgtcctgt gaccagaaga tgtactcctt cgcaccctcg gaaccgggaa
      601 tctccctcgg cgcaaaccga tcgagatcct gcaccatcgc cctggcatca tcaccgcttc
      661 cggaaaggta ggcaatgtgc ttgccgtctg gggagatcac cggtgtggtc gcgcctcctt
      721 ccggtgcaaa cgtgaacctc tgaaccggcg cctcggaaac cgtgcccctc ctgctgagcc
      781 acatcgtcac gatgatgccc accgcgacgg ccaggcccgc gatgaaggcg attctctccc
      841 aaggcaggag ccgcctcgtt ctggtgggcg gtggttccgg ctggccggga gcgggctccg
      901 ccaggtcctc tccaagcaat tccagtgggg caacgaagcg gtaaccccgg cggggaagtg
      961 tctccacata gcgcgggctg tcggctgagt cgcccagcgc ttccctcaac ttgttcacgg
     1021 ccgtgttcag gctgtggtcg aagtccacga aagtatccgt cggccagagc ctctgacgca
     1081 gatcctcccg cgtgacgacg tctcccgggc gctcaatcaa caccgccaga atttgtaaag
     1141 gctgctcctg gattcgtatc ttcagcccgt tcttgcgcaa ctcccccgcc tgcaggtcta
     1201 cttcgaagac tccgaaacgg atcgcacgcg ccgatcttcc acccatcact agccccgatg
     1261 aggttccgcc cgttgcggct tggacggatt atatcacccg gtcagggtct ttccaaagac
     1321 gttctccttc aatgtctaag atgggcaagt ctcgttttac gagcagctta gctgatgatg
     1381 accggctgat ggacagttga ggaactcctg agctgccgcc tgatggaggt acccgctcac
     1441 ctgaccaatt ctgggctcac agcgcgaaag cgcagaggtg agccaaatga actgggtttt
     1501 caggaattgc tggactgtag gttttctgct ggcgacgaat gcgccatctg ctgaacaacc
     1561 ggtacatccc gatatcacgg tttggattta caactacgcc gggatcgacg gcgaggcgtt
     1621 ggcgcgggcc aaggaggcgg cgggccgaat tctcagccgg gcaggaacag aagccgagtg
     1681 ggtcgattgt ccggtgatgg cctctgaggt cgacaagtat ccggactgcg tgagactgcc
     1741 gcaattcctt ggactggtac tccggctccg tccggggagt gcccccaagt ctctcgtggg
     1801 ccgctccact gtattcggtt acgctctcat cccggaaggg ggcggcttcg gcacgtacgc
     1861 tgacgtatat gccggcggcg ctgatttgct ggcgaacgga ggtgaatcgc tcaggggcgt
     1921 catgctgggc catctcatgg cgcatgaact ggggcacctt ctacttggca cttcgaaaca
     1981 ttctgctgcc ggcatcatgc ggagtccttg gagcgggcta gagacggaac gtgcagccca
     2041 aggccggctg ttatttaacc gggcggaggc ggaaagcatg cgaaccaatc ttgcggcccg
     2101 cttgcggcat cagtaggcgg gtcggcaagc aaatggcggc agtccgctct cattctgagg
     2161 gcgacaccca ccgcttgagc cccttcgcgc cgatgtcttt gcgatactgc atcccgtcga
     2221 accggatctt cgccaccgcc gaatacgtct tctcgatcgc cgccgccagc gtctccccgg
     2281 atgccgtcac ccccagcacc cgcccgcccg ccgtcatcac cccgtcggga ccggctcttg
     2341 tccccgcgtg
//
LOCUS       DATIFQ010000300         8155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_480418, whole genome shotgun sequence.
ACCESSION   DATIFQ010000300 DATIFQ010000000
VERSION     DATIFQ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480418"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VLE22_14045"
     CDS             <1..1305
                     /locus_tag="VLE22_14045"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSB15575.1"
                     /translation="LGMTRLNVYAGPAGFYLIRGGPAGDKTVLDSRFGTPAVFPGPAP
                     KANDMFPPNKTYYEIPIAIQDRSFNTDGSLFYPDTRAFFDEVEGPFIPGSDLSPIWNP
                     EFFGNMIMVNGNTWPFQTVEQRRYRFRFLNGCQSRFLILDFNNIPGVEVWQIGNEGGF
                     LAAPVNITANNGNRLLMGLAERADMIVDFTNVPVGSYVLGNVGPDEPFGGGIPGIDFD
                     PADPASTGQVMEFRVVPAVAPDPTTPPQFLVLPPIAPLPAETNTRHLALVEKASEFFE
                     DSPAEAGLGTVDGDPNVAPGVLKTRLWMDPVTENPAIGDTEVWEIYNTTADAHPMHIH
                     EVVFEVVNRQPIIVEEEGGEGGEEEEETVIVDAASPPTGPEPWETGFKDTVISYPGQV
                     TRVRLKFDTPGQFVWHCHIVEHEDNEMMRPYRIGPVQPGQPE"
     gene            complement(1338..1565)
                     /locus_tag="VLE22_14050"
     CDS             complement(1338..1565)
                     /locus_tag="VLE22_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15576.1"
                     /translation="MESLWTTVRPCRGSFEDTDGLRYALEVKADSLFEEVDLAPMFAL
                     NEIIWKSVRGADSEMPLPVHRYWFAGRSAQR"
     gene            complement(2027..3169)
                     /locus_tag="VLE22_14055"
     CDS             complement(2027..3169)
                     /locus_tag="VLE22_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15577.1"
                     /translation="MTELPSCGPLVIDNWRASGDSQEPPEVLEYPLFTDAWMTGGTTA
                     FGPYELFNTIAHAGMPRDSLKPALVLRVQYHFRHELPSMEKTNVGSYHGGELQDEVAA
                     LVSLVLGIRLKAGPASREFPPHGSDPRGVPIAYYGFREPVLITNSCGRMLPNALGAHS
                     LDSLKRFPILPELTQSDGTALVRSARLYQESVWIAESDPAMAWLLMVSAVECAAHRWD
                     QRYETPVERLRASRPDLAKILEERCPDLIDEVALRLVESLGATQKFVKFLLRFLPEPP
                     PARPPVTFRVQWTSRQLKRILSMVYKYRSQALHAGIPFPAPMCEPPRRVAHDWPAPPE
                     KPLGLAASARGGVWVAKDVPLHLHVFEYLARGALLNWWQSLTGQNP"
     gene            complement(3273..3872)
                     /locus_tag="VLE22_14060"
     CDS             complement(3273..3872)
                     /locus_tag="VLE22_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15578.1"
                     /translation="MATPVDQRHPAEVARERLEFHTFIAPDHVERTLIGGVEWRKEFT
                     DVRLHITNHNDFDLENVDFLVRFDTEVAKAAQLSEIPDVNLLAISGDPPGLVYSSLKY
                     KDASGKEVEEEPAFRAIAVPAYRVLCKRLLRGHPLQIVFATAALETEEKDGVSLTAID
                     RFAYPRKRPTTMQISGVYEGTNTDGKHIHRDIGTGTFKY"
     gene            complement(4193..6652)
                     /locus_tag="VLE22_14065"
     CDS             complement(4193..6652)
                     /locus_tag="VLE22_14065"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HSB15579.1"
                     /translation="MRTRLLVSLLLACLGAPASQLPRAVPEGYELPNGWRITPLGKAI
                     PTEDMLLNLSVAPDGKAVIATHGGFNPHGLVVIDPASETAKQRIPLKSAWLGLAWRPD
                     GRKLYVSGGNASGRNPTAAPVYVFDYAEGKLSPKPVSELHETVDMTQIYWSGLAHHPS
                     KPVLYAANRGGSNLAGHVVLFDTGSGALKKRIPVEIAPYDLVLSPDGGTLYVSNWASD
                     SVSVIDTAAERVVARIPVDDNPNDMALHADGRLFVSCANDNSIVVIDTAVRKPVERIS
                     VSVHPRAPAGTTPNALALDAAGKMLFVANADNNAIAVINVAEKGESDVLGFLPAGWYP
                     SSLALPGGKAPKLYVGNSKGMGSYSNIRGPNSPLPPGDEGKGSVKSLQKGSVNIMPLG
                     DLRAQLAGWTKQVYANTPYRDDLLQRAVPAKGPTIVPEHVGVGSPVKHVIYIIKENRT
                     YDQVFGDIPKGNGDPRITIFGRNVTPNQHAIAEEWVLLDNLYCDGEVSVDGHSWSNSA
                     YATDYNEKTWPPQYGGHSRTVITNAYRAKLHLWDLAAEKGLTYRSYGEYAVRSSDGTQ
                     MEAAPGVGRLLGHVAPRFKLPGMRDTDNVREFIREFDEYETHYDSPDPEKRLPNFVVM
                     SLPENHTRGTRPGELTPTAMVANNDWAVGMLVERVTHSRYWPETAIFIIEDDAQDGSD
                     HVDARRTVGLVVSPYVRRGAVDSTLYTTSSMLRTIELLLGLPPMTQYDAAATPMYASF
                     MEKPDLEPYRHVKPQVDLDARNTQTAWGARESMEMELDEVDLAPMFALNEIIWKSVRG
                     ADSEMPLPVHRYWFAGRPAQR"
     gene            complement(6659..7732)
                     /locus_tag="VLE22_14070"
     CDS             complement(6659..7732)
                     /locus_tag="VLE22_14070"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HSB15580.1"
                     /translation="MATQRLVSLDVFRGLTMASMIMVNNPGSWEDVYAPLLHSKWHGW
                     TFTDTIFPSFLWIVGVAMTFSFAKRVERGDDKRRLMLHALRRAAIIFGIGLFLNAFPY
                     FRLATLRIPGVLQRIAICYLIAAVIFLYTGLRGRVIALVSVFVLYTLLMQPGGFERAD
                     NFAKHVDSIFLTGHMWGATKTWDPEGIVSTLPSIGTALFGILVGMLLRSGMGAAEKTA
                     WMFFSGNVLLFLGAAFHGVQPINKNLWTVTFSVLMAGISLLVFACCHWLVDVQGWKRW
                     SKPFVVYGMNAITVYVLSGILADCLSLNLFGGRSLHDILYQNVFLAVASPINASLLYA
                     LANVAVLYVVAWVMYRRGWFVRF"
BASE COUNT         1626 a         2559 c         2364 g         1606 t
ORIGIN      
        1 ctgggcatga cccggctcaa cgtctatgcc ggtccggctg gtttctacct catccgcgga
       61 ggtccggctg gcgacaaaac cgtcctcgac tcccgctttg gcaccccggc cgtattcccg
      121 ggcccggcgc ccaaggcgaa cgacatgttc ccacccaaca agacctatta cgagattccg
      181 atcgcaattc aggaccgttc gttcaacacc gacgggtcac tcttctaccc tgacacgcgc
      241 gcgttcttcg acgaagtgga aggtccgttt ataccgggca gcgacctttc gccgatctgg
      301 aaccccgagt ttttcggcaa catgataatg gtgaacggca acacctggcc gttccagacc
      361 gtggagcagc gccgctaccg cttccgtttc ctgaatggat gccagtcgcg cttcctcatt
      421 ctggacttca acaacatccc tggcgtggaa gtgtggcaga tcggcaacga aggcggtttc
      481 ttggcggcgc ccgtgaacat cacggcgaac aacggcaacc ggctgctaat ggggctggcg
      541 gaacgcgccg acatgatcgt cgacttcacg aacgttccgg tgggcagcta cgtgctcggc
      601 aacgtcggtc cggacgagcc ctttggtggt ggcattccgg gcattgactt cgatccggct
      661 gaccctgcca gtacaggcca ggtgatggag ttccgtgtcg tgccggcggt cgcccccgac
      721 ccgaccacgc cgccgcagtt tctggtgctg ccgcccatcg ccccccttcc ggccgagacc
      781 aacacccgac acctggcgct agtcgagaaa gcgtcggagt tcttcgaaga ctcacctgcg
      841 gaagctgggc tcggcacggt cgacggagat ccaaacgtcg ctcctggtgt cctgaagacg
      901 cggctatgga tggatcccgt caccgagaat ccggctatcg gcgacacgga ggtctgggaa
      961 atctacaata cgaccgccga cgcccacccg atgcatatcc atgaggtggt cttcgaggtt
     1021 gtcaaccggc agccgatcat cgtcgaggaa gagggaggag agggaggaga agaggaagaa
     1081 gaaacggtta tcgttgacgc cgcatctccg ccgactggac ctgaaccatg ggaaaccggc
     1141 ttcaaggaca cggtgatttc ctacccggga caagtcactc gcgtacggtt gaagttcgac
     1201 actcctggac agttcgtctg gcattgccac atcgtggagc atgaggacaa cgagatgatg
     1261 cggccgtacc gcattggacc cgtgcagccg ggtcagccag agtagtagtc tgctcgaggg
     1321 cgggacgccg gtcgccgtca gcgctgagcc gatcgccccg cgaaccagta gcgatgcacc
     1381 ggcaggggca tctcggaatc cgcgccccgc acgctcttcc agatgatctc gttcagcgcg
     1441 aacatcggcg ccaggtcgac ctcttcgaat agcgagtcgg ccttgacctc gagggcgtac
     1501 ctgagtccgt ctgtgtcctc gaagctgccg cggcagggcc gaacggtggt ccagaggctc
     1561 tccaaagccc cagctgggga accgtgaatc gaccactgcc tagtgaagag ccctgctgtt
     1621 tgatgccgat cggctatcgt tggcggaagg catctgactc ggcggctgtc tgggcagatt
     1681 catcagactg attccaggac tctcgttgct ttaatagaat ggcgcttccg gctgtttagt
     1741 ggcttcgtag ccaagtcgaa accaccacgc taaaagtaga tggcttgagc gagcttctgt
     1801 ttggccgcga aagtccgcgc tttgtcattt acccgcccgg atcttggcgt tgatggattt
     1861 gctggctgcg tcgtgaaccg gcgcacaagt gacaatgagc atgtgttcgg gcgcatccct
     1921 tctcctgaaa ataccggtga gcggccggcc tacatccccg tggctctggc tttgcgttga
     1981 tgccggagag cgggtagtga atgaggggcc tcagcttcag cggatgtcat gggttttggc
     2041 ctgttagaga ctgccaccag ttaagaaggg caccgcgggc aaggtattcg aagacgtgaa
     2101 ggtggagcgg aacgtctttt gcaacccaga cgccgccccg cgcactcgct gcaagcccaa
     2161 gtggtttttc tggcggtgcc ggccaatcgt gagcgacgcg acgcggaggt tcacacatgg
     2221 gtgccggaaa cggaatgccg gcgtgcagtg cttgggagcg gtatttgtag accatagaca
     2281 aaattcgttt caattgtctg cttgtccatt gcacacgaaa ggttaccggt ggccgagccg
     2341 gcggaggctc cggcagaaac cgtagaagga acttcacgaa cttctgggta gctcccagtg
     2401 actcaacaag gcggagcgca acctcgtcga tgagatccgg acagcgttct tcgaggatct
     2461 ttgcaaggtc aggtctggag gcacggagtc gttccaccgg cgtctcatag cgctgatccc
     2521 agcgatgcgc ggcgcattca accgcagaaa ccatcagaag ccacgccata gctggatctg
     2581 attcagcgat ccaaaccgat tcctggtata gtcttgcgga ccgaacaagg gctgtaccgt
     2641 cagactgcgt gagctcgggc aggatcggga atcgtttgag actgtctaaa gaatgagcac
     2701 ccaaggcatt tggcaacatg cggccacaac tgtttgtgat caataccggc tcgcggaagc
     2761 cataatacgc gatcggcaca cctcttggat cgcttccatg cggtggaaac tcgcgacttg
     2821 cggggccagc cttaagtcga atacccagga cgagtgacac cagtgcggca acctcgtcct
     2881 gaagttcacc gccgtggtag ctccctacat tcgtcttctc catcgatggc agttcgtgcc
     2941 ggaagtggta ttgaactcgc aggacaagcg ctggcttgag agaatccctg ggcatcccgg
     3001 catgagcaat cgtattgaac aattcatatg gtccgaacgc tgtagttcct ccggtcatcc
     3061 aggcgtcagt gaacaggggg tattcaagga cctcaggcgg ttcctgggaa tccccggagg
     3121 cgcgccaatt gtcgatgacc agaggaccgc aactcggcaa ttcagtcatg accacccttt
     3181 ttgtgataac cggagtgcac cgctcgtcgg cacgcaacaa aaatccgcag atgggggttt
     3241 ctgagggaca agcacaccgg tctcattagt acctaatact taaacgtgcc ggtgccgatg
     3301 tcacgatgga tgtgttttcc atctgtgtta gtcccctcat aaacaccaga gatctgcatc
     3361 gttgtcgggc gttttcgtgg gtacgcgaaa cgatcgattg ccgtgagaga aactccatcc
     3421 ttttcttcgg tttctagcgc agccgtcgca aacacgattt gcaggggatg accgcgcagc
     3481 aacctcttgc acaaaactct ataggctggc acagcgatgg ccctaaaggc gggctcctct
     3541 tcaacctcct ttccgctggc gtccttgtac ttcagggagc tgtaaacaag gcccggggga
     3601 tcacctgata tagccaagag attgacatct gggatttcgc tcagttgtgc ggctttggca
     3661 acctcggtgt cgaacctgac caggaaatca acattctcaa ggtcaaagtc gttgtggttg
     3721 gttatgtgca ggcggacgtc tgtgaactcc ttccgccact ctaccccgcc aatcaacgtg
     3781 cgttcgacat gatcgggcgc gatgaacgtg tgaaattcca ggcgctccct ggctacctcg
     3841 gctggatgcc tctgatcaac tggggttgcc ataactggag ctactcttgg cttcggaggt
     3901 gacgcccagt aactcgccag taagcaacag ccaacaacaa tcagacttcc accgacaatc
     3961 actctccaat cccgtatgcc caggccaatg atcccacaat agcagccgcc atcagtagca
     4021 agctagcaat gcgatggctg aatctgccag tcatcgcaat agacgccaag aacaggccaa
     4081 taaacaagcc aaacagaggg ctctcgatga gccgcacgag cgtttccggc acggaggtat
     4141 tctacctcgc cgtgtaggtt cagtgattcg ttgtatgcag cggtcccgcc tgtcaacgct
     4201 gagcgggccg gcccgcgaac cagtagcgat gaaccggtag cggcatctcg gagtccgcgc
     4261 cccgcacgct tttccagatg atctcgttca gcgcgaacat cggtgccagg tccacctcgt
     4321 cgagctccat ctccatggat tcccgcgctc cccacgccgt ctgcgtgttt cgcgcatcga
     4381 ggtccacctg cggcttgacg tggcggtacg gttcgaggtc cggtttctcc atgaagctcg
     4441 cgtacatcgg cgtcgccgcc gcgtcgtact gggtcatcgg cggcagaccg aggagcagct
     4501 cgatcgtccg cagcatcgag ctcgtcgtgt aaagtgtgct gtccactgct ccgcgccgca
     4561 cgtatgggct gaccaccagc cccaccgtcc gccgcgcgtc cacgtggtcc gatccgtcct
     4621 gcgcgtcgtc ttcgatgatg aagatcgccg tctccggcca gtagcggctg tgcgtcacgc
     4681 gctccaccag cattccgacc gcccagtcgt tgttcgccac catcgccgtc ggcgtcagct
     4741 cgcccggccg cgtaccccgc gtgtggttct ccggcaggct catcacgacg aaattcggca
     4801 ggcgcttttc cgggtccgga ctgtcgtagt gggtctcgta ttcgtcgaac tcacggatga
     4861 actcgcgaac gttgtccgtg tcgcgcattc ccggtaactt gaaacgcggc gcgacatgcc
     4921 caagcagccg gcccaccccc ggagccgcct ccatctgcgt gccgtcgctc gaccggaccg
     4981 cgtactctcc atagctgcga taggtcaggc ccttctccgc cgccaggtcc cacaggtgca
     5041 gttttgcgcg gtacgcattc gtgatcaccg tgcggctgtg cccgccatat tgcggcggcc
     5101 acgtcttctc gttgtagtcg gtggcgtacg ccgaattcga ccacgagtga ccgtccacgc
     5161 tcacctcacc gtcgcagtac aggttgtcga gcagcaccca ctcctctgcg atggcgtgct
     5221 gattcggtgt cacgttacgc ccgaaaatcg taatccgcgg atctccgttg cccttcggga
     5281 tatccccgaa gacctggtca tacgtgcggt tctccttgat gatgtaaatg acgtgcttca
     5341 ccggcgagcc caccccgaca tgctccggca cgatggtcgg ccccttggcc gggaccgccc
     5401 gctggagcag atcatcccgg tacggcgtgt tcgcgtacac ctgcttcgtc caacccgcaa
     5461 gctgtgcgcg cagatccccg agcggcatga tattcacgct gcccttttgc aggctcttga
     5521 cggacccttt cccctcgtcg ccgggcggca gcgggctgtt cggcccccgg atgttcgagt
     5581 acgagcccat gcccttgcta ttgcccacgt aaagtttcgg cgccttgccg ccgggcagcg
     5641 ccagcgagga cgggtaccac cccgcgggca ggaatcccag cacgtcactc tcgcccttct
     5701 ccgcgacgtt gatcaccgca atcgcgttgt tgtcggcgtt cgccacgaat agcatctttc
     5761 cggccgcgtc gagcgccagt gcattcggcg tcgtgcccgc gggcgcgcga ggatgcaccg
     5821 acaccgaaat ccgctccacc ggcttgcgta cggccgtgtc aatcaccacg atgctgttgt
     5881 cgttggcgca ggaaacgaac agcctgccgt ccgcgtggag cgccatgtcg ttcgggttgt
     5941 catccaccgg gatgcgcgcc accacccgct cagcggcggt atcgatcacg ctcaccgaat
     6001 cgcttgccca gttggaaacg taaagcgtgc cgccgtcggg cgacagaacg aggtcgtacg
     6061 gcgcgatctc aaccggaatc cgtttcttca gcgcgccgga gcccgtgtcg aacagcacta
     6121 cgtgtcccgc cagattgctt ccgccccggt tcgccgcata caaaaccggc ttcgacgggt
     6181 ggtgcgccag ccctgaccag tagatctgcg tcatgtccac cgtctcgtgg agttccgaca
     6241 ccggcttcgg cgaaagcttc ccctcagcgt aatcgaaaac gtaaaccggc gccgcggtcg
     6301 gattccggcc gctggcgttt ccaccgctga cgtacaactt ccggccgtcc ggacgccacg
     6361 ccagacccag ccacgcgctc ttcagcggga tgcgttgttt cgccgtctcg gaggccggat
     6421 cgatcaccac cagcccatgc ggattgaatc ctccgtgggt cgcgatgaca gccttgccgt
     6481 ccggcgccac cgagaggttc agcagcatgt cctcggtcgg aatggccttc ccgagcggcg
     6541 tgatgcgcca cccgttcgga agctcgtatc cttccgggac ggcgcgagga agttgagacg
     6601 ctggggcacc caggcaggcc agcagcaggg aaacaagaag ccgggttctc attgctagtc
     6661 agaatctcac aaaccagccg cggcggtaca tcacccacgc caccacgtac agcaccgcga
     6721 cgttcgccag cgcgtaaagc agcgacgcgt tgatcgggct cgccaccgcc aggaacacat
     6781 tctgataaag gatgtcgtga agcgagcggc caccgaacag gttgagagac aggcagtccg
     6841 caagaatgcc cgagagaacg tacaccgtga tagcgttcat gccgtataca acgaatggct
     6901 tgctccaccg cttccagcct tgtacgtcca ccagccagtg acaacaggcg aagacgagca
     6961 gagagatccc cgccatcagg acggaaaacg taaccgtcca gagattcttg ttgatcggct
     7021 gcacgccgtg aaatgccgct cccaggaaca gaagcacatt gcctgagaag aacatccatg
     7081 ccgtcttctc ggccgctccc atccccgaac gcagcagcat cccgactaga attccgaaca
     7141 gtgccgtgcc gatcgatggc agcgtgctga cgatgccctc ggggtcccac gtcttcgtcg
     7201 caccccacat atggccggtg aggaagatcg agtccacgtg cttcgcaaaa ttgtccgccc
     7261 gctcaaaccc tcccggttgc atcagcagcg tatagaggac gaacacgctt accagcgcaa
     7321 tcaccctgcc ccgcagcccg gtgtagagaa aaatgaccgc cgcgatcagg taacagatcg
     7381 cgatccgctg cagcacgccg ggaatccgca gcgtggccag ccgaaagtac gggaaggcgt
     7441 tcaggaacag tccgatcccg aagatgatcg ccgccctccg gagcgcgtgc agcatcagcc
     7501 ggcgcttgtc gtcgccgcgc tcgacgcgct tggcgaagga gaacgtcatc gccacgccga
     7561 cgatccaaag gaacgacggg aagatggtgt ccgtgaaggt ccacccgtgc cactttgaat
     7621 gaagcagcgg cgcgtagaca tcctcccacg accccgggtt gttgaccatg atcatcgacg
     7681 ccatggtcag cccgcgaaac acatccagcg aaaccagccg ttgcgttgcc atgagttcag
     7741 aagagtatac cgcggccttc agagcacctg atgcacgccg cccggattcg ttcgttccgg
     7801 cggcccttcc caaggctggt gccgttccga cggtagcgcc tcgctgtgtg gcgctggctt
     7861 cttagccggg aaaagttgga acggcactgg cccgtttgga atttgttaaa gtacgggttc
     7921 cgggttgtcg gccgcggcat gcggtgccgg tggcttcgat gcctgaattc aaacgaagca
     7981 ggttctaatg cgccacgtcc tattgcacta tcatgtcctg aaaaacgcag gctcgaccgt
     8041 cgagttcatt ctcgatcgca gcttcgggaa caaatggact cggttcgatg ggccagaccg
     8101 gaactcggtt ctgaatatcg ggcacttgtt ctcgttcctc gaagagaatc cggac
//
LOCUS       DATIFQ010000301        11852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2013937, whole genome shotgun sequence.
ACCESSION   DATIFQ010000301 DATIFQ010000000
VERSION     DATIFQ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013937"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..2726)
                     /locus_tag="VLE22_14075"
     CDS             complement(129..2726)
                     /locus_tag="VLE22_14075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB15581.1"
                     /translation="MFWLDRFRSRRFERDLDDELKFDLECRTQDNISAGMTPVEAHRQ
                     ALLSLGGLEQTKEECRDVRPLAWIEVVTQDVRYGLRMLRRNPGFTLIAALSLAVGIGA
                     NSAIFSFIDAIATRPMPVPRVSEIIHISTTSPQFPEAGVSGADYLDYRNASSLAGVAA
                     VDYRGPVLTIGDVPEGTYAEVVSDNFFTLLGIEAAVGRTFREETIPPPVIVLGHRFFQ
                     ARFGGDPGIIGRGVRMGGRIVTIVGVAPASFRGTRNDMSADLWVPFSTWGSWVLHERR
                     IPQLAVIGRPRTGVSINRAKQELTSIASNLARAYPATNKGRTVRVESDRAHRMRAAGS
                     FSLVLMALVGAVLLLASMNVAILLLARAETRRREMAVRLALGAGRVRLVRQLLTEASL
                     LAALGAVAGIALGGILIQVLPSVLRLGYFVGQAELLLDSRVLLFSVFVATGSGLLFGL
                     APAIISARADLAPVLKGAEVAAGTSGSRVKARNVLVVLQVGIALVLVVAGILLARSFR
                     NGLKADLGFSHNNVLVLEIPNLGEAGTGQRDLLYRQVTASIRALPGVHSAAVAMRPPL
                     GGSGGGRTERILLPGPRPEPVEVRMSVVGPGYFSLLGTRLLRGREFDDHDGFSGRKVA
                     IINETGARRLWPSEDPIGKLFQTGGPDGPAWEIVGVSRDARINSISEAPSPYLYFPFA
                     QLNSGDVNLLVRTDSSPATLVKPVRDQLKEIDSGLVVYASFTLSSLVDSALGEFRLPS
                     QLASALALVGVLLAAVGLYGVISYFVSARTRETGIRMALGARPGDVLSSVLGLTARLA
                     ACGIALGIVFTLAAGKGLSSRLYGVSARDWSALAAASTLMLLVALAAGFVPARNATRT
                     DPALTLRHD"
     gene            complement(2737..3072)
                     /locus_tag="VLE22_14080"
     CDS             complement(2737..3072)
                     /locus_tag="VLE22_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSB15582.1"
                     /translation="MSDSPKTDLLQGTLDLLILKVLAPGPMHGFAISQRIQQVSRDIL
                     QVQQGSLYPALHRLERRKWVSAEWAASENNRRAKYYRLTAAGRRRLEEEVSNWERIAS
                     AVSLILENA"
     gene            3228..3527
                     /locus_tag="VLE22_14085"
     CDS             3228..3527
                     /locus_tag="VLE22_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15583.1"
                     /translation="MPPYVMVLRVVLGVLSVFFAYSLGKAIARKRRNQIRDSVLTAWV
                     LRTAVTGFGVFWRVGADALSIGVVVLAVLSAAAGYRNEGKQKVREDLTDVIFPKQ"
     gene            complement(3560..3772)
                     /locus_tag="VLE22_14090"
     CDS             complement(3560..3772)
                     /locus_tag="VLE22_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15584.1"
                     /translation="MRVRLRRFGGILTLTGPNMPPEEKSWYQKEDEFWNRLHERDASR
                     RGSIWWILAVIVLGLMALGGLRGPFT"
     gene            complement(3769..4383)
                     /locus_tag="VLE22_14095"
     CDS             complement(3769..4383)
                     /locus_tag="VLE22_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15585.1"
                     /translation="MGIKDTVIWAVVMAAEVGLAAGRAQQGSQAEGGPLPIFVHDMAG
                     VPDEVLSTARGEMARILSDAGVRVAWLDSANGRPAGTIILRIVPATLDYVDKAALGLA
                     LLSGPEAVYATISYPRVQRCIARQFISTASAGQVLGHAMAHEVGHILLGAGSHSENGL
                     MRPTWDERALNDVSKGILGFSRDEARHIQGEVDRRTGGDATRGK"
     gene            4511..5077
                     /locus_tag="VLE22_14100"
     CDS             4511..5077
                     /locus_tag="VLE22_14100"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB15586.1"
                     /translation="MAVSAPEVTQLLVAWGQGDQAARDSLIPLVYQELRRLAHRYMTG
                     ERPGHTLQTTALVNEAYLRLVDCRRVRWQNRAHFFAVSATLMRRILVDYARSRGYQKR
                     GADAQTVLLDENLDLSPQKGCDLVAIDEALDALAVFDPRKSKVVELKFFGGLTADEIA
                     EVLQVSPQTVLRDWKLARSWLLREIKRG"
     gene            5082..7919
                     /locus_tag="VLE22_14105"
     CDS             5082..7919
                     /locus_tag="VLE22_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSB15587.1"
                     /translation="MMPDRWRRIEQIYNSALSREGGERASYLAAACAGDEELRREVER
                     MLGNAGQSTGPLDGHALDATARDCVGAVAPDLSGMKLGRYQVIVRLGEGGMGEVYRAR
                     DTRLGRDVALKVLRPESVADPERKRRFEQEARAASALNHPGIITIYDIDQAEGIDFIA
                     MECVPGQKLGERIGRKGLPVAEALKYAILMADALAAAHAAGIVHRDLKPANVMVTDEG
                     RVKILDFGLAKLAERESGDVVTTQTNTGTVMGTVAYMSPEQAQGKHVDARSDIFSFGA
                     LLYEMLTGSRAFQRDSVAETLAAILDKDPRPASEIAPAVPHDLDKLISRCLRKDPERR
                     IQHMDDVKLALLELKEESEPGVSGRDAGSIRKGRKHIWWAAGVLGAVAIAAGIWFAIS
                     WHQLVDAPLKAVPFTMYQGWEADPSFSPEGSRVAFCWNGVEQDNWDVYVKLIGEGEPH
                     RLTDDPADDFSPAWSPDGRQIAFLRRQRDHLELLSVPSVGGLETKLFELSLATPDRDY
                     PNREVAWSPDSKHLVFYDKPASDEPPGLFLLSIVTREKRRLTTCPIPLMRESDPSFSP
                     DGRTLAFTRAVTYSFRDLYLLSLSPDLRPSGEPRLLVSQHQRLSQPVWTPDGREIVFS
                     AAEGLWRVAVRGSGRPARLPFVGDQLDISRQGRRMVYNLPAWDSNIWRVELSSQREIT
                     PPVSFIASTQTDTNPQYSPDGQKIVFASDRSGNFEIWLCNAGLSNAKQLTTLNASESG
                     SPSWFPDGRRIVFDSDKEGNFDIYVVDADGGAPQRLTSDPADDSTPSVSRDGKTIYFS
                     SRRTGSREVWRMPAEGGAPIQVTRKGGLCPFEAGDVLYYQKSEGRFSEVWRVPVAGGE
                     ETKVLQSVGGRRFAVLPDGIYFIEWPDFRSVPSLRFFSFATGRTTKVATLAGPFLFWD
                     FGLTVSPDGRFALYTHPDHPNSDLWLVENFH"
     gene            complement(8007..9344)
                     /locus_tag="VLE22_14110"
     CDS             complement(8007..9344)
                     /locus_tag="VLE22_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15588.1"
                     /translation="MSRPRPKLRFFICSRDRPQALGLCVESLSRSLREAAPEFSAHCY
                     ILDDSTTPALSGEAHRRATAGASRELHLEIIDRARQHAVYRKLTGLNLESCRLLRSTC
                     RMLGQGPWDLAGVRNFALLIAYCYSSDDDLIVFLDDDILLTSSAYGGHFIEIDGASVI
                     RQLLSSTPPGRLVASGTEYFGRFDGSILDHLRLLFDDLSNGCLLGLWEAESRVQTTAR
                     LKQASLFPSTLPTRLALPGAPVLKHGPGISGALLATTPAALFSHPLPRCYNEDWIWLS
                     LLGRPGAAIRRVDPKALHAAPPQPPIESRFVAYQNTGEVVYRAVRAVMNDAPSRCNAL
                     EWCENAISVDHFLWAADALVREIQSLLQTGAKIDNSLNGTGPLSGEPHLCASVRRAIR
                     RIRRCGQDTLESVEAMDHAALHRWFRKYLRRVPVWRRLLQESREVLLTNARGS"
     gene            9435..10145
                     /locus_tag="VLE22_14115"
     CDS             9435..10145
                     /locus_tag="VLE22_14115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HSB15589.1"
                     /translation="MDELVSCVVATRDRPRFFRQLVRCFLRQTYRHSELIVVDDGERP
                     VAQLRSRSPRVRYVRLSRLTPTGTKLNIGIKQARGTVIQKLDDDDYYHPDFLKLAVSR
                     LPDKNRAHCMVGWDCFLVLLAGEARVRHSGHGWVAGGTFCFSRKLWERAKFRDVTVDE
                     DDWFLRDLQPEVVPVCAPEHYILVRHGNNTWKRMRNRQRVDDFFRGLPEYGKPLEDLV
                     DPDAQAFYRSLARGKCVE"
     gene            complement(10274..10651)
                     /locus_tag="VLE22_14120"
     CDS             complement(10274..10651)
                     /locus_tag="VLE22_14120"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSB15590.1"
                     /translation="MLTPEQDQLTQRVDEWYEAIRSGNLAKLDEILHPNYTNTEATGA
                     VLTKQTDLANFESGRYKVLELTPTRIGPPVVHGNSARVDGEDVCTATFNGQTATNTYQ
                     WTDTWVKDDSGNWLCVASHSWKI"
     gene            11288..>11852
                     /locus_tag="VLE22_14125"
     CDS             11288..>11852
                     /locus_tag="VLE22_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15591.1"
                     /translation="MRKLTDPPIGSLGDLDPALSPDGRTLVFIRSPGAGVNSLHFLSL
                     SEDLRPVGEPRRLGLPHRTVNSPAWTADGREVVFSAGYIGQKGLWRVAASGPGEPKPL
                     ALPAEKAVTPRISKDGRRLVYAAVSVAYNLWRMEIPPRENTPPRRITPSTQWDTDPQY
                     SPDGRRIAFTSLRSADGEIWTCDPDGSN"
BASE COUNT         2346 a         3590 c         3697 g         2219 t
ORIGIN      
        1 gacacgtccc gatgacgagc gcttcgcccg catcctcaag gtcttcgccg acgcccgcga
       61 gaaactccgg cgtcgcatgt gaatcacaaa ccggagacac accagcgact cacaattcct
      121 ccggcaaatc aatcatggcg gagggtcaac gccgggtcag tgcgggtggc gttccgggcc
      181 gggacgaagc ctgcggcgag cgcaaccaga agcatcagtg tggaagccgc ggcgagagcc
      241 gaccaatccc ttgcgctcac gccatagagc cggctcgaaa gacccttccc cgccgccaga
      301 gtgaatacga ttccgagcgc gataccgcaa gccgccagcc gggcggtaag accgagcacg
      361 gagctgagta cgtcgcccgg acgggccccg agcgccattc ggatgccggt ctcacgggtg
      421 cgcgcgctga cgaaatagga aatgacgccg tagaggccca cggcggcgag caagacaccc
      481 acgagcgcga gggcggatgc aagctgtgaa ggcaagcgaa actccccgag cgccgagtca
      541 acgaggctgc tcagagtgaa cgaagcatac acgacgaggc ccgaatcgat ctccttgagc
      601 tgatctcgca cgggcttgac gagggtcgcc gggctcgagt cagttcttac gagcaggttc
      661 acgtcgccgg agttcagttg agcgaatgga aaatacagat acgggctcgg cgcttcgctt
      721 atggagttta tgcgagcgtc acgggacacg cccacgatct cccaggctgg gccgtccgga
      781 ccgccggtct ggaatagctt cccaatcggg tcttcggagg gccacaggcg gcgggcgccg
      841 gtctcgttga tgatagccac cttgcgcccc gagaagccgt cgtggtcgtc gaactcgcgg
      901 ccgcgcagta gccgtgtgcc gagcagcgaa aaatagccgg ggccgacgac cgacatccga
      961 acttcaaccg gctctggtcg gggtccgggc agcagaatcc tctcggtgcg gccaccgccg
     1021 gagccgccca acggcggtct catagcgaca gcagcgctat gcactcctgg cagagcccgt
     1081 atgctggcgg tgacctggcg atagagcaaa tcgcgctgcc ccgttccggc ttcgcccagg
     1141 tttgggatct ctaggacgag tacgttgttg tgactgaacc cgagatcggc tttgagcccg
     1201 ttacggaagc tgcgcgcaag caggataccc gcgaccacca gcacgagcgc aatgccgact
     1261 tggagcacca ccagcacgtt ccgagccttg acccggctgc cggacgttcc agccgccact
     1321 tccgcaccct tgagtaccgg agcaaggtcc gcacgagcag agataattgc tggcgccaag
     1381 ccgaaaagca acccacttcc cgtagcaaca aagaccgaga acagaagcac gcgggaatcg
     1441 agcaggagtt cggcttgccc gacgaaataa ccaaggcgaa gaactgacgg caacacctgg
     1501 atgagaatgc ctccaagagc gatgccggca accgctccga gagctgcgag aagggaggct
     1561 tcagtgagga gctggcgcac gaggcggacc cggccggcgc cgagtgccag gcggacggcc
     1621 atctctcgcc tccgcgtctc ggcgcgcgcc agcaatagta tggcgacgtt catcgaggca
     1681 agaagcaaca ccgcacccac gagcgccatc agcaccaggc tgaagcttcc ggctgcacgc
     1741 atgcgatgtg cgcgatcgga ttccacacga acggtccggc ccttgtttgt ggccgggtaa
     1801 gcacgcgcca agttcgatgc gatgctcgtg agctcctgct tcgcccggtt gattgaaacg
     1861 ccggtccgcg gccggccgat gaccgcgagc tgcgggatgc ggcgttcgtg caaaacccag
     1921 gaaccccatg tgctgaaggg gacccagaga tcggccgaca tgtcgttgcg cgttccccgg
     1981 aaggacgcag gggcaacgcc cactatggtc actatcctgc cacccattct cactccgcgg
     2041 ccgatgatcc cgggatcgcc gccgaatctt gcctgaaaga accggtggcc gagaacgatg
     2101 acgggaggcg ggatcgtttc ctcgcgaaac gtgcgaccga cagcagcctc gatgcccaag
     2161 agcgtgaaga agttgtcgga gacgacttcg gcgtaggtgc cttcaggaac gtcgccgatt
     2221 gtgagcaccg gaccgcggta atccacggcg gctacgccgg caagcgagct ggcgttgcga
     2281 tagtccagat agtcggcgcc ggatacaccc gcctctggga actgcggcga agtcgtgctg
     2341 atgtggatga tctccgagac gcgaggcacg ggcattgggc gcgtggcaat tgcatcgata
     2401 aaactgaaga tggcgctgtt ggcgcctatg cccacagcca gcgagagtgc tgcgatcagc
     2461 gtgaagccgg ggttgcggcg cagcattcgg agaccgtaac gcacatcctg agttaccacc
     2521 tcgatccagg cgagcgggcg cacgtcgcgg cactcctcct tcgtctgctc caagccgccg
     2581 agcgagagca gagcctggcg gtgcgcctcg acgggggtca tgccggcgct gatgttgtct
     2641 tgggtgcggc actccaggtc gaatttcaac tcgtcgtcga ggtcgcgctc gaaccgacgg
     2701 ctacgaaatc gatcaagcca gaacatgttg gctcctctaa gcgttttcga gaatcaggga
     2761 cacagccgag gcgatgcgct cccagttcga tacttcctct tccaggcggc ggcggccggc
     2821 ggcggtcagc cggtaatact tcgccctgcg attattctcc gatgcggccc actccgcgct
     2881 cacccactta cgacgttcca gacggtggag cgcgggatag agagagccct gctgcacctg
     2941 caggatgtcg cgggagacct gctggatacg ctgggagatg gcgaatccgt gcatcggtcc
     3001 cggggcgaga acttttagga tgagcagatc gagagtgccc tggagaagat cggtcttcgg
     3061 cgaatcactc atgaagacat tctacaggag tttcatgtag aatgtcaagg tgaaggtgcg
     3121 ggaatcgatc aggccgcaac gccggagcgt gccggggtgg tctcttcacg ccgtctccgg
     3181 tttcattccc cttgcatcac cggcagcgtt gcgccagatt gatagggatg cccccgtacg
     3241 tgatggtcct gcgtgtcgtt ctcggggtgc tcagcgtttt ctttgcgtac tcgcttggaa
     3301 aggccatcgc ccggaaacgg aggaaccaaa ttcgcgattc ggtcctgaca gcgtgggtcc
     3361 tgcgaactgc ggtcacgggc ttcggagtct tctggcgcgt gggcgccgat gcgttatcaa
     3421 tcggagtggt tgtccttgcc gtattatcgg cggcggcagg ttaccgaaac gagggcaaac
     3481 agaaggtgcg ggaggacctg acggacgtca tcttcccgaa gcagtgagac cgcggcgcac
     3541 ggccgcgcta gcggcgttgt caggtaaagg ggccgcgtaa cccgcccagg gccataaggc
     3601 cgagcacgat caccgcgaga atccaccaga tcgagccccg gcggctggca tcccgctcat
     3661 gcagccggtt ccagaattcg tcctctttct gataccagct cttctcctct ggaggcatat
     3721 tcggacctgt tagggtcaga ataccaccga accggcgcag ccggactctc attttcctcg
     3781 tgtggcgtcg ccgccggtcc gccgatcgac ttcgccctgg atatgccgag cctcgtcacg
     3841 agagaagcca agaattcctt ttgacacgtc attgagcgcc cgttcgtccc atgttggccg
     3901 catcagcccg ttctctgagt gggaaccggc accaagcaga atgtggccga cctcgtgggc
     3961 catggcatga cccaagacct gtccagccga cgccgttgat atgaactggc gggcaataca
     4021 tctctgtacc cgggggtacg aaatggttgc atagaccgcc tcggggccgc tcagcagtgc
     4081 caagccgagc gccgccttgt caacgtagtc gagcgtcgcg ggcacgattc tcaggatgat
     4141 cgtgccggcg ggacgcccgt tggcggagtc aagccaagcg acgcggacgc cggcgtccga
     4201 caaaatcctc gccatctccc cccgggcagt cgacagaacc tcgtccggaa cacccgccat
     4261 gtcgtgaacg aagatcggca gagggccgcc ttccgcctgg ctaccttgtt gggcccggcc
     4321 cgcggcaagg ccaacctcag ccgccattac caccgcccaa atgaccgtat ctttgattcc
     4381 catgtttcct ccccgtccgt ccgggaaggc gggaatcagg accgaaaacg acaatcgcgc
     4441 ttaagacacc ttggtccggt gttatactga ggcaggccaa ccgaaaggcc gcggtcaggc
     4501 aggagagggg atggccgtgt ccgcgccgga agtgacgcaa ttgctggtcg catggggcca
     4561 gggggaccag gcggcgcggg attcgcttat cccacttgtc tatcaagagt tacgccgtct
     4621 ggctcaccgc tacatgacgg gtgagcggcc agggcacacc cttcagacca cggcgctggt
     4681 gaacgaagcc taccttcgcc tggtggactg ccggcgggtc cgctggcaaa accgtgctca
     4741 cttctttgcg gtgtcggcca ccctgatgcg gcgcattctg gtggattacg cccgctcccg
     4801 cgggtaccaa aagcgcggag ccgacgcgca aaccgtgtta cttgacgaga atctggacct
     4861 gtcgccgcag aagggctgcg acctggtggc gatcgacgag gcgctcgatg cactagccgt
     4921 cttcgatccc cggaagagca aggtggtcga actgaagttc ttcggcggtc ttacggccga
     4981 cgagattgcc gaggtgttgc aggtttcccc acagaccgtc cttcgggact ggaagctcgc
     5041 ccggtcctgg ctgctgcgcg agataaagcg gggctgaatc catgatgccc gatcgctgga
     5101 gacggatcga gcagatctac aattcggcgc tgtcccgaga agggggcgag cgggcatcct
     5161 atcttgccgc ggcttgcgcc ggggatgagg aactgcgccg cgaggtggag cggatgctcg
     5221 gaaacgcggg gcaatccacc ggacccttgg acggtcacgc tctggacgct accgcccgcg
     5281 attgcgtcgg ggcggttgcg ccggatctgt cggggatgaa actgggccgg taccaagtga
     5341 tcgtccgcct tggtgagggt gggatggggg aagtgtaccg ggcacgagac acccggctcg
     5401 ggcgggatgt ggcgttgaag gtgttgcggc cggagagcgt ggccgacccc gagcgcaaac
     5461 ggcggttcga gcaggaggcg cgggctgcct cggcgctgaa ccatcccggc atcattacga
     5521 tctacgacat cgaccaagcc gaaggcatcg acttcatcgc catggagtgc gtgccgggtc
     5581 agaagctggg cgagcggatc ggccgcaagg gactgccggt ggctgaggcg ctgaagtacg
     5641 ccatcctgat ggcggacgca ctagcggcgg cgcacgccgc gggcatcgtg caccgtgact
     5701 tgaagccggc caacgtgatg gtcaccgacg aggggcgtgt gaagatcctc gacttcggcc
     5761 tggcgaagct ggcggagcga gagtccggcg acgtcgtgac cacgcagacc aatacgggaa
     5821 cggtcatggg cacagtggcc tacatgtcgc cggagcaggc gcagggaaag cacgtggatg
     5881 cgcggtcgga cattttcagc tttggggcgt tgctgtacga gatgctgaca gggagccggg
     5941 cgtttcagag ggactcggtg gctgagacgc tggcggcgat tctcgacaag gatccacggc
     6001 cggcgagcga gattgccccc gcagttccgc acgacctgga caagctgatc agccgctgcc
     6061 tgcgcaagga ccccgagcgc cggatccagc acatggacga cgtaaagctg gctttgctgg
     6121 agttgaagga ggaatccgag cccggcgtct ccggtcgtga cgcgggatct atcaggaagg
     6181 gacgcaagca catctggtgg gccgccggtg tgctaggagc cgttgccatc gccgccggaa
     6241 tctggtttgc catttcctgg caccagttgg tagatgctcc actcaaagcg gtccccttca
     6301 ccatgtatca gggctgggag gcggatccga gcttctctcc cgagggtagc cgtgttgctt
     6361 tctgctggaa tggggtggag caggacaatt gggatgtcta cgttaagctg atcggagaag
     6421 gagaacctca tcggctcacc gacgatcctg ccgatgactt cagtcccgcc tggtcacctg
     6481 atggccgcca gatcgctttc ttgcgtcgcc agagggacca ccttgaactg ctttcggttc
     6541 cctcggtcgg tggtctggag accaagctct ttgagctaag tctcgcgact cccgatcgtg
     6601 attatccgaa tcgcgaagtt gcctggtcgc ccgatagtaa gcacctggtg ttttacgaca
     6661 aacctgcgtc ggatgagcct cctggcctgt tcctgctgtc tattgtaact agagaaaaac
     6721 gcaggctgac gacgtgtccg ataccgctga tgagagagtc agatccctcc ttttcgccgg
     6781 acggccggac cctggccttc acgcgcgcag tcacctattc cttcagggac ctttacctgc
     6841 tctccctctc gccggacttg cgaccatccg gggaaccaag gctcttggtc tctcagcacc
     6901 agaggctgtc tcaacctgtt tggaccccgg atgggcggga gatcgttttc tccgccgctg
     6961 aggggctgtg gagggtagcg gtgcgcgggt ccggaagacc tgcgcggtta ccgttcgtag
     7021 gagaccagct cgacatttcg cggcaggggc gccgtatggt gtataactta ccggcctggg
     7081 acagcaacat ctggcgcgtt gagctttcgt cacagaggga gattactcct ccagtgagct
     7141 tcatcgcttc gactcaaacg gacacgaatc ctcagtactc gccggacgga cagaagatcg
     7201 tgtttgcttc cgatcgttcg ggaaacttcg agatttggct ctgcaacgcc ggcctctcga
     7261 atgcaaagca gctcaccaca ctcaatgcgt ctgagagtgg gagccccagt tggttccccg
     7321 acggccggcg tatcgtcttt gattccgaca aagagggcaa cttcgacatc tatgttgtcg
     7381 atgccgatgg cggcgcgccc cagcgcctga ccagcgatcc cgccgacgac tcgacgccga
     7441 gtgtctcccg ggacggcaag acgatctatt tctcatcaag aagaacgggc tcgagggaag
     7501 tttggcgcat gccggcggaa ggaggggccc ccatacaagt gacccggaaa ggcgggcttt
     7561 gtcctttcga ggccggcgac gttctttact atcagaaatc cgaggggcga ttctccgaag
     7621 tctggcgagt tccggtggcc gggggtgaag agaccaaggt cctccagtcc gtagggggcc
     7681 gccgatttgc agttctcccc gacggcatct acttcatcga atggccggac tttcgtagcg
     7741 ttccctcgct tcggttcttt agctttgcca ctggccgaac cacgaaagtc gccacactcg
     7801 ccggtccctt cctcttttgg gattttggcc tcaccgtatc gcccgatggc cgcttcgctt
     7861 tatacacgca tccggatcat ccgaactcgg acctgtggct ggtggagaac ttccactgac
     7921 cgctccgcgt tatgccagcg cggcccgcta agcttctgct ccccccggtc gcggctcggc
     7981 aactgcacgc gcacgcgggg ccaagattag gaaccgcgcg cgttggtgag taggacctct
     8041 cgactctcct gcaatagccg ccgccatact ggaacccgcc gcagatattt ccggaaccac
     8101 cgatgcagcg ctgcgtgatc catagcttct acggattcga gagtgtcctg cccgcaacgc
     8161 cggatcctgc gaatggcgcg tctcaccgat gcgcacaggt gaggctctcc cgagagtggt
     8221 cccgtcccgt tgagcgagtt gtcgattttc gccccggtct ggagtagcga ctgtatttct
     8281 ctcaccaggg catccgccgc ccacaagaag tgatccacag agattgcatt ctcacaccat
     8341 tcaagggcat tacagcgcga aggagcgtca ttcatgacag cccggaccgc acgataaacc
     8401 acctcgccgg tgttttgata ggcgacgaaa cgactctcga tagggggttg cggtggagct
     8461 gcgtggagcg ccttgggatc tacccttcga atggcggctc ccggccggcc gagcagacta
     8521 agccagatcc aatcttcgtt atagcagcgg gggagaggat gcgaaaacaa agcagccggc
     8581 gtggtggcga gcagcgctcc gctgatgccc ggtccatgct taaggaccgg cgcgcccggg
     8641 agtgccagcc gcgttggcaa tgtcgatgga aacaaactgg cctgtttcag gcgtgccgtt
     8701 gtctgaactc tggactcggc ctcccacagg ccgaggagac aaccattgct tagatcgtcg
     8761 aaaagcaggc gtaagtggtc caatatcgag ccatcaaacc gcccgaaata ctccgtacct
     8821 gaagcaacca gtcgcccggg gggagtactg gagagcaatt gccggatgac ggaggctccg
     8881 tcaatttcta tgaagtggcc cccataggcg ctgctggtaa gcaagatatc gtcgtccaga
     8941 aatacgatga ggtcgtcgtc actggaataa cagtatgcga tcaagagcgc gaaattccgt
     9001 accccggcga gatcccaagg gccctggccg agcattcggc atgtggacct cagaagccgg
     9061 caggactcga gattcaagcc tgtcagtttc cgatagacgg catgttgccg tgcccgatcg
     9121 atgatctcga ggtgcagctc ccgagaggcg ccggcggtgg cacggcggtg cgcttcgccc
     9181 gagagcgcag gcgttgtcga atcatccaaa atgtagcagt gcgcggagaa ctctggcgcg
     9241 gcttcccgga gggatcggct caggctctcg acgcaaagcc ccagggcctg cggtctatcc
     9301 cggctgcaaa taaagaagcg gagctttggg cgcggcctgc tcaatcgcaa aactcgccga
     9361 cggcagtttc ggatattgtc tcacgatccc gacaggatag ttctgaatgt caatcagagc
     9421 cctataatgt ggcggtggac gaacttgtct cctgcgtcgt ggcaaccaga gaccggccac
     9481 ggttctttcg ccagttggtc cgctgtttcc tgcgccagac ttataggcac tcggaactga
     9541 tcgtcgtgga tgacggagaa cggccggtgg cgcaattgcg ttcccgctcg ccgcgcgtgc
     9601 gttatgttcg gctgtcccgg ttgaccccca ccggcacgaa gctaaacatc ggaatcaaac
     9661 aggcaagggg gacggtgatt cagaaactcg atgacgacga ttactaccat cccgacttcc
     9721 tgaaactcgc cgtctcccgc ttgccagaca agaatcgagc tcattgcatg gtgggctggg
     9781 attgtttcct cgtcctgctg gcaggggagg ctagagtccg tcattccgga catggctggg
     9841 tggctggagg cacgttctgc ttttcccgta aactctggga gcgggcaaag tttcgcgacg
     9901 taacggtaga tgaggacgat tggtttctca gggatcttca gcccgaggtc gtaccggtct
     9961 gcgccccgga acactacatc ctcgtccggc atgggaacaa cacgtggaag agaatgcgaa
    10021 accggcagag agtggacgat ttcttccgag gcctgcccga gtatgggaag ccgctggaag
    10081 acttagtgga tccggacgca caggcgttct accggtcact ggcgcgcggg aagtgtgtcg
    10141 aatagagagg cgaacacaga ttcgcagagc cggggcccgc agatccggcc aacgccgctt
    10201 gcgcggacct gtaccgctta ctccctatgg agaccaaata tgagactgaa ctgccggcga
    10261 tcggctactt ttgtcagatc ttccagctgt gcgaggccac acacagccag ttgccgctgt
    10321 cgtctttgac ccaggtatcc gtccattgat acgtattggt ggcagtctgg ccgttgaacg
    10381 tcgcggtgca cacgtcctcc ccgtcgaccc gggcggaatt tccgtggaca acaggtgggc
    10441 ctatccttgt gggtgttaac tctaatacct tgtaacggcc ggactcgaag ttagcgagat
    10501 ccgtttgctt ggtcaagacg gcccccgtag cttccgtgtt cgtgtaattg gggtgcagaa
    10561 tctcgtcgag tttcgcaagg ttgccactcc ggatggcctc gtaccactcg tccacccgtt
    10621 gtgtcagttg atcctgctca ggtgttagca tacgcactcc tttcgcaaat tttatggccg
    10681 agaacgtgaa atacgcgctt agctgtgagg acaaattgcc gtcgtattcg ccttagacaa
    10741 agccaaccgc ccaagcctgc ggaggtcgac acactcagaa acctcctctt gaggggcgtt
    10801 caaaagcagg tccattgccc cagatcggct ggaacgggaa gtgagtggac tttagtttca
    10861 agctgttcga ggatagccgc gctaattgta ctccgggtcc gagtctatga caagagccct
    10921 catttttctc tcatttttct cgtgtgacgt cgccgccagt ccgccgatcg accgcatcct
    10981 gaattcggcg cgcctcatcg cgcgagaagc cgaggatccc tcttgatacg tcgttgagca
    11041 cacggctgtc ccatgttggg cgcatcaagc cgttctccga gtgagaacca acacccagca
    11101 gaatgtggcc gacctcgtgg gccatggcat gacccaagac ctgtccagcc gacgccgttg
    11161 atatgacctg gcgggcaata catctctgta cccgggggta cgaaatagtt gcatagaccg
    11221 cctccgggcc tctcagcaat gccagcccgg acgggggaga agaggataac accgatacac
    11281 ccgagaaatg cgaaagctga ctgacccgcc catcggatcc ctcggtgatc tggacccggc
    11341 gctctctcct gacggaagaa cgctggtgtt catccgaagt cccggcgcgg gcgtcaatag
    11401 tctccatttt ctctcccttt ccgaagacct gcgccccgtc ggggaaccac ggcgcctcgg
    11461 cctcccgcac cgtaccgtca actccccagc ctggaccgcg gacggccgcg aagtcgtttt
    11521 ttctgccggc tatataggtc agaagggact atggagagtg gcggcctccg ggcccggtga
    11581 gccgaagccg ctggcattgc ccgctgaaaa agcagtcacc cctcgaattt cgaaggacgg
    11641 gcgtcgcctt gtgtatgccg cggtgagcgt tgcgtacaac ctgtggcgga tggagatacc
    11701 ccctcgcgag aacactcctc cccgccggat cacgccttcc actcagtggg acactgaccc
    11761 acagtattct ccggacggga gacgaatcgc attcacctcc cttcgctcgg cagacggcga
    11821 gatttggaca tgcgaccccg acggctccaa cg
//
LOCUS       DATIFQ010000302        18418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_481444, whole genome shotgun sequence.
ACCESSION   DATIFQ010000302 DATIFQ010000000
VERSION     DATIFQ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 18418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18418
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481444"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VLE22_14130"
     CDS             complement(<1..802)
                     /locus_tag="VLE22_14130"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HSB15592.1"
                     /translation="MKIHTKAVHAGDRKKAPRQVPVTTPIYTASSFFCESLEDLDRIF
                     AREEDGFCYSRYDNPTNAALEELVTALEEGHGTLACASGMAAVQTAVLTALTDRPKRI
                     VAADALYGATISLLFKVLEPLGVETRLVDICDLDAVDKAIGAHKPGCVLMETVSNPLL
                     RVGEMDRVAVLARSAGAALVVDNTFATPCLVRPLQLGAGLVVHSATKYLAGHGDVLGG
                     VVVSDNDHYETLRTLSRAYGPVLGPFESYLTMRGLKTLPLRIERQCANA"
     gene            complement(804..2204)
                     /gene="cysS"
                     /locus_tag="VLE22_14135"
     CDS             complement(804..2204)
                     /gene="cysS"
                     /locus_tag="VLE22_14135"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HSB15593.1"
                     /translation="MALRFYNTLSQRVEDFAPLHDNVVRMYTCGPTVWNFAHIGNLRT
                     FTFVDILRRWLRYRGYRLDHVMNITDVDDRIIRESLAAGRTLTEYTEEYTKAFLEDTG
                     ALRLERPERLVKATEHIPQMVALVRRLIDGGFTYSSDGSVYFRIASFPGYGKLSHTDF
                     SGIRAGVRVDTDRYDKADARDFVLWKARKEGEPSWDTEIGAGRPGWHLECSAMAMAYL
                     GETLDIHAGGVDLTFPHHENEIAQAEAATGHTFARFWLHSEHLMLDAQVMSKSLGNFL
                     TLRDVVAQGYKPEAIRYLLSSVPYRKQLNFTFDALKSADTAIERLRNYKLRLDTGKFP
                     GGESEKLAARTAEALKRIEESLDDDLNTAEALGAVFEYIRETNTAMDAGEFPQGNAAS
                     ALDLLARFDSVFDVLRPTVTGGVLADAEIESLIAERNKARKTKDFVRADRIRAELLER
                     DVILEDTKDGVRWKRK"
     gene            complement(2253..3011)
                     /locus_tag="VLE22_14140"
     CDS             complement(2253..3011)
                     /locus_tag="VLE22_14140"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HSB15594.1"
                     /translation="MNRPVVILLALTLLSAQAQNTSDVYSTAKRPRALALIGDRYHSP
                     VYIRDSLSPALVRENIPVTFIEEVTALNAEALKSHQLLIILRDGMNWPNGYDKPHVQW
                     MTDAQQKAIWDFVQEGGGFLALHNAQGIYPPGGLYYKLFGGDYGGHPPPYVFTIRVEN
                     KNHPITAGVEDFEIFDEQHTVKYYLDREHLLLRSLAKDNLAAEAGWWREVGKGRFCYL
                     APGHTTEALTHPMMQRLIRNSVRWLLRMDERAAR"
     gene            3067..4209
                     /gene="tgt"
                     /locus_tag="VLE22_14145"
     CDS             3067..4209
                     /gene="tgt"
                     /locus_tag="VLE22_14145"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSB15595.1"
                     /translation="MPHFEVVAECPATGARAGLLHTAHGVVETPVFMPVGTQATVKGL
                     MQRDLAGELDAHILLANTYHLFLRPGHEVVRRLGGLHRFMSWDRAILTDSGGYQVFSL
                     DSLRKVSDDGVLFRSHLNGDAHMFTPESTVDVQLALGSDILMVLDECLPYPVSQESAK
                     QSIQRTISWAGAAFRHFRRRVAEEPEALAGRRLLPIVQGSMFPDLRRDCTAALLELDA
                     EGYAIGGLSVGEPRTLSLEMVEVTAPLLPWDRPRYVMGVGMPEELPEYVARGMDMMDC
                     VLPSRNARNGCLFTSKGRIIIKHARYKEDPLPVDGDCGCYTCRNFSRAYLRHLFQAGE
                     ILYATLATLHNIRRYLDIMRQVRQSIVFGTFPEYLRAVRSAPVEIA"
     gene            4313..4618
                     /gene="yajC"
                     /locus_tag="VLE22_14150"
     CDS             4313..4618
                     /gene="yajC"
                     /locus_tag="VLE22_14150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSB15596.1"
                     /translation="MVLSFLVQGQAPSNPILGFLPILLIFAIFYFLLFLPMQRQRKQQ
                     QKLLRELQPGNLVVTSGGVMGTIVSLDDETVILRVKPDNLKIQVTRSSVAGLVPEKK"
     gene            4635..6251
                     /gene="secD"
                     /locus_tag="VLE22_14155"
     CDS             4635..6251
                     /gene="secD"
                     /locus_tag="VLE22_14155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSB15597.1"
                     /translation="MQKSLRWRWTLIAIVLLLSVYGVIGLPKSMAELKTNLDKNIRLG
                     LDLKGGSHLILQVQVQDAAKVEADQAIERLKDELRKAGVNYASMERNDPTTIEQADSI
                     QIDIRGVPAEKSGDLRRIVSERFATWVLTPVSSTDYRMNMRPSELLALKSDTVERSIR
                     TIEQRINGLGLTEPVIQQHGRTDAEFEILVQLPGVDDPARVKQIMQTAAMLEISEVKD
                     GPFASPEQAMAKHGGVLPLNTKLVKMAPGKSEGDSWYLLTRTPVITGRDLRTARPGRD
                     EFQKWEADFTLSQDGARRFGRFTEANIGNRLAVILDNQIRSVATIQARIEDSGRITGL
                     SGEQEASDLALVLRAGSLPAGIVYLEERTVGPSLGSDSIRQGVVAGLVGLALVILVML
                     VYYRKAGVNAVVALILNGVITIAMLGYFDAVWTLPGIAGLILSLGMAVDSNVLIFERI
                     REEMRAGKAAPSAVSAGFDRVFLTIIDTHVTTVVACAFLFMFGTGPVKGFAVTLVIGL
                     IANVFTAVFVSRTMFEFSFYRNPKMAELSI"
     gene            6339..7553
                     /gene="secF"
                     /locus_tag="VLE22_14160"
     CDS             6339..7553
                     /gene="secF"
                     /locus_tag="VLE22_14160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HSB15598.1"
                     /translation="MELFKETSIDFLSRKWLALGISLALIGATFVSLIAKGGPNYGID
                     FRGGTLVYVKFAGTPPVQQIRSALGQRMSGEVTVTTVRDAAMGKADEVVIGTELRDER
                     ALDAARVTITKTLEERFGNTGSKLDLNNASRQELADRLRDPLLRAGTGLSEEQLGGLV
                     DNLRNFRDKNRSGLVRNLDELSAVPGVTPAILNVIKQEVAVAPFSIRQVEIVGPKAGG
                     DLRRQAILATLYALGGMLVYIAFRFELIAGVAAVLAIIHDTLITIGFFSFFDREISLT
                     VIAAVLTLIGYSMNDKIVIFDRVRENLKVMHRMPLPDLVNKSINQTLSRTVITAGPTL
                     LCTLALYYLGGPVLNGIAYALFIGIIVGTYSSVFIASTILVFWQNFLESRKRRTVPVA
                     AAAGREPRKTVK"
     gene            7594..8358
                     /locus_tag="VLE22_14165"
     CDS             7594..8358
                     /locus_tag="VLE22_14165"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSB15599.1"
                     /translation="MFEQTFIEGTGKTRKPLTVFVSFILQLLLVGIAVLIPLIYTDTL
                     PRTQLTSFLVAPPPPPPPPPPPAAVPVKVVKVIPRQFDAGRLMAPKTIPKEIAMIKEE
                     ELPPPTSGAVGVIGGVPGGVPGGTPGGVIGGIIGAVPTAAPPPPPPVKEAPKPVVPQR
                     IRVGGNVQQAKLVRQPRPVYPPLARQARIQGTVRLTAVIAKDGTIQELQVVNGHPLLV
                     PSALEAVKQWVYQPTLLNGEPVEVITQIDVNFTLSQ"
     gene            8404..9141
                     /locus_tag="VLE22_14170"
     CDS             8404..9141
                     /locus_tag="VLE22_14170"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSB15600.1"
                     /translation="MLLQMQVWAFLLLQEGAVGWDIRSLWAQMGIPAKLVVVVMFLMS
                     AWSIGVMLDRMLAFSGARKQSRQFAPAVAGALREGKLDEAIKIADRYKKSHLAKVVVA
                     GLQEFRAHELSHDIPGEDIEASKRALERAEAIVHAELKRGTAGLATIGSTAPFVGLFG
                     TVVGIINAFKGISTEKSTGLGAVAGGISEALVATAIGLFVAIPAVWMYNYFAGKVEGF
                     DVEMSNSSSELIDYFLKRSQQRSGASK"
     gene            9267..9725
                     /locus_tag="VLE22_14175"
     CDS             9267..9725
                     /locus_tag="VLE22_14175"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSB15601.1"
                     /translation="MAKPKATPPVSDINVTPMVDVMLVLLIIFMVVTPMLSKGVSVEM
                     VRTKNPIAMQAADKEDAVLVALTREGRVFLGTTQMKADELAPKVKDLLVNKLDKTVYI
                     RSDARAKYEKVVEVVDNLRAAGVDQVGLLTELIQEKKGGAAAAAAAPPAG"
     gene            9760..10188
                     /locus_tag="VLE22_14180"
     CDS             9760..10188
                     /locus_tag="VLE22_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSB15602.1"
                     /translation="MAMAVGGPGGGPKSDINMTPLIDVLLVLLIIFMVITPLTPRGLD
                     ALVPQPPPPGQQADESRQRTVVIVINRDRSMMINAEPTDEGRLGPRLEEIFKTRAERV
                     VFVKGDPDLEFQFVARAIDIAKGAGIDKIGLMTAKVEAGL"
     gene            10234..11046
                     /locus_tag="VLE22_14185"
     CDS             10234..11046
                     /locus_tag="VLE22_14185"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB15603.1"
                     /translation="MNRKVITVLGVILTLLLVVAGTGCQKLRARDQLNKGVNAFRNAS
                     YPEAVEFFKQAVELDPQFPTARLYLATAYMMQYIPGAESDENMRMAQAAHDHFVQVLQ
                     TDPKNTVALASIALLYFNQKKLDEAQEWYQKLIEADPKYKEAHYTLGVIAWTKTFQPR
                     MEARAKLGMRPEDPGPIKDKKVREALAEKNLPVVESGLDHLKKALEIDPEYDDAMAYM
                     NLLFRERADLQETQGAYNKDTEEADNWVQKTLDTKKIKASRTPQGGITAEGQ"
     gene            11130..13358
                     /locus_tag="VLE22_14190"
     CDS             11130..13358
                     /locus_tag="VLE22_14190"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HSB15604.1"
                     /translation="MASEPTTAIPRTPEAIGNMPAVDGHYLSLRSFLQELRPKEDFTP
                     LDQAYRFAAKHHGAQTRTSGEPYMVHPLEVTRILADMNMDMLCLQTGLLHDVVEDTGT
                     GLEEIRRVFGEEVAHCVDGVTKLSKLSVYSAEDRQAESVRKMLLAMVNDIRVILVKLA
                     DRVHNMRTLDCLSRERQERIARETLEIYAPIAHRLGMGKIRGELEDLSFHYLEPESAG
                     ELARLIESKRQENEAYLQQIKGAIEAKLRAEGIPARVEGRLKRAYSVYQKMKRQKIAV
                     DQVYDLMALRIVTDSVKNCYAALGVIHHEWHPIPGRIKDFIAIPRPNLYQSLHTSVVG
                     PEGRTFEVQIRTEEMHRIAEEGIAAHWKYKEGNRGQSDEIQRIAWLRQLVEWQQEMRD
                     PSEFMSTLKVDLYPEEVYCFTPRGKVIALPRGATPIDFAYSIHSDVGHTCVGAKVNGR
                     IVTLKYNLRNGDVVEILTQPGHVPSKDWLALVKTSRARNKIKHVINVTERIKAIEIGE
                     KLFEKEARRLGISLKRIAREDLDRVAGEYGCSKMEDVHAALGFGRFSARQVLQKLVPQ
                     ATAETQPGEQAKAPPPGPIQPSLVDATDLVIKVRGIDDLMVYRAKCCNPILGEAIVGY
                     ITRGKGVAVHSSSCPNVQNLLYEVERKIDVEWARSGAEPFPVKMIIHTDDRPGMLHQL
                     TSILSDEESNIRTLEARMDDQHGGDGALIDMTVDVRDKKQLERIVTSMRRLSGVRDVE
                     RI"
     gene            13355..13723
                     /locus_tag="VLE22_14195"
     CDS             13355..13723
                     /locus_tag="VLE22_14195"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HSB15605.1"
                     /translation="MMPASHGATEFVPLEPEHIAVSKSKGIKIDWKDSHRSEYSLRFL
                     RDHCPCATCSGAHGTEPMKPESASPFQMYKPTLKMLGVEPVGNYAIRIQWNDGHDTGI
                     FSWEYLRRICGCPECTGEAG"
     gene            complement(13733..14758)
                     /locus_tag="VLE22_14200"
     CDS             complement(13733..14758)
                     /locus_tag="VLE22_14200"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSB15606.1"
                     /translation="MAEQEKNDEVRVGDTQDGLPVLTADDHLKHLLPLETATPWYVSV
                     VRNLKDLIFPEKLPPLKLTSKPVAVKDIWGFYGGYKARAGIGSVLIHVSVVTLAFSLA
                     ANDTIRNKVKETAISLVAPDIAPYIPQTPKPQPMGGGGGGGDRSPLPASKGRLPKLAP
                     RQFTPPMAVVNNPNPKLIMEPTIVVPPDVNLPNVNMAQYGDPLGKIGPPSNGPGSGGG
                     IGSGSGGGVGPGRGPGYGPGEGGGFGGGVFRVGGGVSAPTLVFKVEPEYSEEARKAKF
                     QGTVVLYCEVTERGTCQNVKVLRALGLGLDEKAIEAVKKWKFRPGAKDGKPVTVAATI
                     EVNFRLL"
     gene            complement(14946..15122)
                     /locus_tag="VLE22_14205"
     CDS             complement(14946..15122)
                     /locus_tag="VLE22_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15607.1"
                     /translation="MPSRPFWFLLTWGCVAWYAVVVVVVGWRGFLEIRRMIQDLEASG
                     PLEPDSNSSEPTAL"
     gene            complement(15132..16577)
                     /locus_tag="VLE22_14210"
     CDS             complement(15132..16577)
                     /locus_tag="VLE22_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSB15608.1"
                     /translation="MTSALGLLDWSIIAIYLAGTMATGLAMRRYVGKVEHFLVAGREM
                     DVSLGIASLAATEFGIVTAMYTAELGYKNGFAGAIPGILLTIAIAAVGWTGFVIKPLR
                     ASGALTIPELLERRFGSRVRLLAGLVMVLGGLLNMGIFLRVGGEFLTIVTGIPMRYLE
                     LTMTGLLLMVLVYTVLGGMLSVLVTDYLQFLVLGGGVVLVTILVAADAGWEKVVAVVA
                     AERGDGAFNPFTSPGMGPGYVLWQALSMLAVVLTWQTYIQRVLSAKDPQTAQRMYTRT
                     SIFFVGRYLIPAYWGMAALAMLGARGESLSPIHAMPAYLTRLLPAGLLGLVVAAMLAA
                     EMSTDSSYMLSWGSILYNDVIRPLKKKRPFSERGGLLVNRVIILGIGLFLLAYGLWYE
                     IPGRAWDYLAITSNIYLSSISVLLVACCYWEGANKTGAIAAIVGGAVSPIAFLLSGLA
                     RHVEVAGLASFGLAAAGMLAGSWAGRRRKAG"
     gene            16647..17927
                     /locus_tag="VLE22_14215"
     CDS             16647..17927
                     /locus_tag="VLE22_14215"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15609.1"
                     /translation="MRTEEKYMVASKHPVSSSHPKRREFLFAAGASPLAAGAVAVQGS
                     APAGGGKPIGVGLIGCGGRGSHLIQTARQLNEVGVPAEVVAVCDIYQPRLERLATRMK
                     AKEYSRSAELIRDPRVNAVIVATPDRLHVHHALEAVRAGKDVYCEKPLTHWAQFDKLK
                     ELVREVRARKTVFQVGAQWVSDPMWARAAEQIRGGVIGKVVHAQTGYFRHGDSGERGM
                     PIDDPNAQAGHGLDWEAFQADAPRRDFTVSRFFQWRMYADYSGGPVTDLYPHPLTRLL
                     KALGVGMPKKVVAFGGKFFYDGGRDVPDTFDMLIEYPQGMTVSLLGTIVNDTMIETVI
                     RGSEGTVKFSGEAQLSVSPQPGAPERRRTIATDRTGLDHMRDFIECVRTRQRPMCDIE
                     LGYAVQTALIMGMRSYVEGRVASFDADKEEIRMS"
     gene            17924..>18418
                     /locus_tag="VLE22_14220"
     CDS             17924..>18418
                     /locus_tag="VLE22_14220"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 family protein"
                     /protein_id="HSB15610.1"
                     /translation="MITRRTFLVGGALFPLTNAAVRSDHVKGEKVSFHQDARLLFEYR
                     YSAEAPKTYVHPFCAPDGNVTLDSPADHVHHRGVMLAWSDVNGFDFWGEQNPGKHGRI
                     VHRRFAGLRKDRLVSLEDWVAEGKTLLTERRTIAVPISSKDAVWLDWTSELSAVSGPV
                     QLSAA"
BASE COUNT         3739 a         5505 c         5626 g         3548 t
ORIGIN      
        1 aggcgttcgc acactggcgc tcgatccgca gcggtagagt cttcaggccc cgcatcgtga
       61 gataactctc aaacggaccg agaaccgggc catacgcccg cgacagcgtg cgcagagtct
      121 cgtaatggtc gttgtcggag accaccacgc ctccgagaac gtcgccgtgg ccggcgagat
      181 actttgttgc gctatggacc actaggccgg ctccaagctg gagcggccgg accaggcagg
      241 gagttgcaaa ggtgttgtcc acaaccagtg cagccccggc cgacctcgcc agcacggcga
      301 cccggtccat ttcgccgacg cgaagcagcg ggttggacac agtctccatc aacacacagc
      361 cgggcttgtg tgcgccgatc gccttgtcta cggcgtccag gtcgcagatg tcgactaacc
      421 gagtctccac gcccagaggt tcgagcactt tgaacagcag gctgatcgtc gccccgtaga
      481 gagcgtcggc ggcaacgatc ctcttcggcc gatcggtgag agccgtcagc acggccgtct
      541 gaacggcagc catccccgac gcgcaggcca gcgttccgtg gccctcctcg agtgcggtca
      601 cgagttcttc caacgccgcg ttggtcgggt tgtcataccg cgagtagcag aacccgtcct
      661 cctcgcgcgc gaagatgcgg tccaggtctt ccaggctctc gcagaagaag ctcgatgccg
      721 tgtagatggg agtggtaacc ggaacttgcc ggggggcctt cttgcggtct ccggcgtgca
      781 ccgccttggt gtgaatcttc aagttacttc ctcttccagc ggacaccatc cttcgtgtcc
      841 tcgagaatca cgtcccgctc aagcagttcg gcgcggatcc ggtcagcgcg gacgaagtcc
      901 ttcgtcttcc gcgccttgtt acgttccgcg atcagcgact caatttcagc gtcggcaagc
      961 acgcctccgg tcaccgttgg ccgcaaaacg tcgaagaccg agtcgaaccg cgccaagagg
     1021 tccagcgcgg acgccgcgtt tccttgcgga aattcgcccg cgtccatagc cgtgtttgtc
     1081 tccctgatgt actcgaaaac cgcgcccaga gcttcggccg tgttgaggtc gtcatccaga
     1141 ctttcttcga ttcgtttcaa ggcctcggcc gttcgcgcag ccagcttctc gctttcgccc
     1201 ccaggaaatt tgcccgtatc gaggcgcagc ttgtagttcc gcaggcgctc gatagcggtg
     1261 tcagcggatt tcagggcgtc aaacgtgaaa ttcagttgct ttcggtacgg taccgaagag
     1321 agcaggtagc ggatcgcttc cggtttgtag ccctgcgcca ccacatcgcg aagcgtgagg
     1381 aaattgccga gcgacttcga catcacttgt gcgtcgagca tcaggtgttc cgaatgcagc
     1441 cagaaacgcg caaacgtatg gccggtggcc gcctcggcct gcgcaatctc gttttcgtga
     1501 tgagggaacg tgaggtcaac gcctcccgca tggatgtcga gcgtctcccc gagatacgcc
     1561 atcgccatag ccgagcactc caggtgccat ccgggacgcc ccgcgccgat ctccgtgtcc
     1621 caggacggtt cgccttcctt gcgcgccttc caaaggacga aatcccgagc gtcggccttg
     1681 tcgtaccggt cggtgtcgac gcggacgccc gcgcggatgc cgctgaaatc ggtgtgcgaa
     1741 agcttgccgt accccggaaa actggcgatc cggaaatata ccgagccgtc gctgctatac
     1801 gtgaagcctc cgtcgatcaa gcgcctgacc agcgcgacca tctggggaat gtgctcggtg
     1861 gcctttacga ggcgctccgg gcgttcaagc cgcaacgcac cggtgtcctc gaggaaggcc
     1921 ttcgtgtact cttcggtgta ttccgtcagc gtcctgcccg ctgcgagcga ttcccggatg
     1981 atgcgatcat ccacgtcggt gatattcatg acgtggtcca ggcggtagcc gcggtaccgc
     2041 agccagcgtc gcaggatgtc tacgaacgtg aacgtgcgca ggttgccgat gtgagcgaag
     2101 ttccagaccg tgggcccgca ggtgtacatc cgaacgacat tatcgtgaag tggcgcgaag
     2161 tcctcgacgc gctgtgagag cgtattatag aagcgcaaag ccattgtttt gccgggtttt
     2221 ctaactatcc taacatcctg cccacgaacg ccctaccttg cagctcgttc gtccatgcgg
     2281 agcagccaac gaactgagtt gcggatcaga cgttgcatca tcgggtgcgt cagagcctcc
     2341 gtggtgtggc cgggcgcaag gtagcagaac cggcctttac cgacctctcg ccaccatccg
     2401 gcttccgcgg ccagattgtc cttcgccagc gaacgaagga gcaggtgctc gcggtcgagg
     2461 tagtacttga ccgtatgttg ctcgtcaaag atctcaaagt cctcgacgcc cgccgtaatc
     2521 ggatgattct tgttctccac gcgaatggtg aacacgtacg gcggcgggtg cccgccataa
     2581 tcgcctccga acagcttgta gtacagtccg ccgggagggt agattccttg tgcgttgtgc
     2641 agcgccagga accctccacc ctcttgaacg aagtcccaga tcgccttctg ctgcgcatct
     2701 gtcatccatt gaacgtgcgg cttatcgtaa ccgttcggcc agttcatgcc gtcgcgaagg
     2761 atgatcagta gttgatggga cttcagggcc tccgcgttaa gggcggtgac ctcttcaatg
     2821 aacgtcacgg gaatgttctc gcggacgagc gctggggaaa gactgtcgcg gatatagaca
     2881 gggctgtggt agcgatctcc gatcaacgcc agggctcgcg gcctcttggc tgtcgagtaa
     2941 acatcggatg tgttctgggc ctgggcggag agcagcgtaa gcgccaggag aatgacgacg
     3001 ggacgattca tcagtggact ccttgccggt ttaacgcgcg tctaattcta caatagtgag
     3061 ttgcctttgc ctcacttcga agtcgtggcc gaatgcccgg cgaccggcgc gcgggccggt
     3121 ttgctgcaca cggcgcatgg cgtcgtcgag acgccggtct tcatgccggt aggcacccag
     3181 gcgactgtta aggggctgat gcagcgtgac ctcgccggcg agctcgacgc gcacatcctg
     3241 cttgccaaca cgtaccatct ttttctgagg ccgggccacg aagtggtccg ccgcctcggc
     3301 ggcttgcaca ggttcatgag ctgggaccgg gctatcctga ccgattccgg cggctaccag
     3361 gttttcagcc ttgacagtct tcggaaagtg agtgatgacg gcgtcctgtt ccggtcgcac
     3421 ctgaatggtg acgcccacat gttcacgccg gagtcgacgg ttgacgtcca acttgccttg
     3481 ggcagcgaca tcctgatggt tctggacgaa tgcctgcctt acccggtcag ccaggaatcg
     3541 gcgaaacagt ccatccagcg cacgattagc tgggccggcg ccgccttccg ccactttcgc
     3601 cggcgggttg ccgaagagcc ggaagcgttg gccgggcggc gtttgttacc aatcgtgcag
     3661 ggttcgatgt ttcccgatct gcgccgcgac tgcaccgccg ccctgctcga actcgatgcc
     3721 gagggctacg caatcggcgg gctctcggtc ggggaacccc ggacgctcag tctggaaatg
     3781 gtcgaggtaa cggccccact gctgccgtgg gatcgccccc gctatgtgat gggagtcggc
     3841 atgcccgagg agttgccgga gtacgtcgcc cgcggcatgg acatgatgga ttgcgtcctt
     3901 ccctcccgga atgcgcggaa cggttgcctg ttcacgtcca aggggcggat catcatcaag
     3961 cacgcgaggt ataaggagga ccctttgccg gttgacgggg attgcggctg ttacacgtgc
     4021 cggaacttct cccgcgcgta cctccggcac ctcttccagg ccggcgagat cctctacgcc
     4081 accctggcga ccttgcacaa tatcagacgc taccttgaca tcatgcgtca agtcaggcag
     4141 tctatagtat tcgggacgtt tccggaatat ttgcgagcag tgcgttcagc gccggtggaa
     4201 attgcctagc cggccggaca tctctctcgt gttttgaatc catccgattc agcgaacccg
     4261 cgaaagctgg ccgcctggac gctatatcct gaaccttgag ccgaggtgaa aggtggtact
     4321 gagttttctc gtgcagggac aagctccctc caacccgatt ctgggttttc tccccatcct
     4381 cctcatcttc gcgatctttt acttcctgct cttcctgccg atgcagcgcc agaggaaaca
     4441 gcagcagaag ttgctccggg aactccagcc gggaaacctg gtcgtgacgt ccggtggcgt
     4501 gatgggcacc atcgtctcgc tggacgacga gacggtgatc ctgcgggtga aacccgacaa
     4561 tctgaagatc caagtgaccc gcagttccgt tgccggattg gtgccggaga agaagtagcc
     4621 ggtgaggtga gcgcatgcaa aagagcttga gatggagatg gacactgatt gccatcgtgt
     4681 tgcttctgtc cgtgtatgga gtaatcggac tgccgaaatc gatggccgag ttgaagacca
     4741 acctggataa gaacatccgg ctcggtctcg acctcaaagg tggaagccac ctgatcctcc
     4801 aggtccaggt gcaggacgcc gccaaggtag aggcggacca agccatcgag aggctgaaag
     4861 acgagttgcg gaaagccggt gtcaactacg cctcgatgga gcgtaacgac ccgacgacaa
     4921 ttgaacaggc ggattccatc cagatcgaca ttcgcggcgt gccggcagag aagtcgggcg
     4981 acctgaggcg catcgtcagc gagcgcttcg cgacatgggt tctgacgccg gtcagctcca
     5041 ccgattaccg gatgaacatg cgcccgtcgg aacttctggc gttgaagtcg gacaccgtcg
     5101 agcgttctat ccgcacgatc gagcagcgca tcaacggtct cggcctgacg gaacccgtga
     5161 tccagcagca cggccgcacc gacgccgagt tcgagatcct tgtgcaattg cctggcgtag
     5221 atgatccggc gcgtgtgaag caaatcatgc agactgccgc catgctggaa atcagcgagg
     5281 tgaaggacgg tccttttgcc agtcccgaac aggcgatggc caagcacggc ggtgtgcttc
     5341 cgctgaacac caagctggtc aagatggccc ctggaaagag cgagggggat agctggtatc
     5401 tgctcacgcg cactcctgtc atcaccggcc gggatctgcg gacggcgcgg ccgggacggg
     5461 acgagttcca gaagtgggaa gcagatttta ctctgagcca ggacggcgcc cgacgtttcg
     5521 gccgctttac agaagccaat atcgggaacc ggcttgccgt gatcctcgac aaccagatcc
     5581 gcagcgtggc gacaatccag gctcggattg aagacagcgg aagaatcacg ggcctctctg
     5641 gcgagcagga ggcgtcggac ctggctctgg ttctccgggc gggttcgctg ccggccggta
     5701 tcgtctacct ggaggagcgc acggtaggcc cgtcgcttgg ttcggactcg atcaggcagg
     5761 gcgtggtcgc cggtcttgtc ggcctggcgc tggtgatcct ggtgatgttg gtttactacc
     5821 ggaaggccgg cgtgaatgcc gttgtcgctc tgattctgaa cggcgtaatt acaatcgcca
     5881 tgctgggata ctttgacgcg gtttggacgc tcccaggcat cgccggctta atcctcagct
     5941 tgggtatggc cgttgacagc aacgtgctga ttttcgagag gatccgggag gaaatgcggg
     6001 ccggcaaggc agcccctagc gcggtttctg ctggatttga tcgtgtcttc ctgaccatca
     6061 ttgacaccca cgtgaccacg gtggtagcct gcgcctttct cttcatgttc ggtacggggc
     6121 cggtcaaggg gttcgcagtg acgctggtta tcggactgat cgcaaacgtg tttacggccg
     6181 ttttcgtgtc ccgtacgatg ttcgaattct ccttctatcg gaacccgaag atggcggagt
     6241 tgagtatctg accgggatgg aattcgtcgc aggagattgg gaactgaaag gcaacagccg
     6301 gtgtccaaat caattggacg ccagcgtgag ctgcgcagat ggaactgttt aaagagacga
     6361 gtatcgattt cctgagccgg aagtggctcg cactcggaat ctcgctggcg ctgatcgggg
     6421 cgacttttgt gagcctgatt gcgaaggggg ggccgaacta cggcatcgac tttcggggcg
     6481 gcacgctggt ttacgtgaag tttgccggca ctcctccggt tcagcagatt cggagcgcgc
     6541 tggggcagcg catgtcgggc gaggttactg tgacgacggt tcgcgacgcc gctatgggga
     6601 aggccgacga agtcgttatt ggaacggaac taagggatga gcgcgcgctc gacgccgctc
     6661 gcgtcacgat caccaaaaca ctcgaggagc gattcggcaa caccggctcc aaactcgacc
     6721 tcaataacgc cagtcgccag gaattggccg atcggctgcg ggatccgctg ctgcgtgccg
     6781 ggaccggact ttccgaggag caactggggg gcctggtcga taacctccga aacttccgcg
     6841 acaagaaccg ctcagggctc gtgcggaacc tggatgaact gagtgccgtt cccggagtga
     6901 cgccggcgat tctgaatgtc attaagcagg aagtggccgt cgcccccttc tccatccggc
     6961 aagtcgagat tgtggggccg aaggccggcg gggatcttcg tcgccaggcg atcttggcga
     7021 cgctttacgc gctcggcggg atgcttgtct atatcgcgtt ccgctttgag ttaatcgccg
     7081 gcgttgcggc ggtgctggcg atcattcacg acacgctcat tacgatcggc ttcttttcct
     7141 tcttcgaccg ggagatttcg ctgaccgtaa tcgccgccgt gctcacgttg atcggctact
     7201 cgatgaatga taagatcgtc atcttcgatc gggtccgtga gaatctgaag gtgatgcatc
     7261 ggatgccgtt gccggatctc gtcaacaaga gcatcaatca gacgttgagc cgtaccgtca
     7321 ttacggctgg tcccaccttg ctatgcaccc tggctctgta ctatttgggt gggcccgtgc
     7381 tcaacggaat tgcgtacgcg ctgttcattg gcatcatcgt ggggacatat tcctcggtct
     7441 ttattgcgag tacaattctg gtgttttggc agaacttcct ggagagccga aagagacgga
     7501 cggtgccggt cgccgccgcc gccgggaggg agccgcgtaa aactgtgaag tgagtccggg
     7561 cgccgtagcc cgcggcttgg aaagggtaac gaaatgtttg agcagacttt catagaggga
     7621 accgggaaaa cgagaaagcc gttgaccgtg tttgtctcgt tcatcctcca gttgctgttg
     7681 gtcggcatcg ccgttctgat cccgctgatc tatacggaca ccttgccgag aacgcaattg
     7741 accagcttcc tggtcgcgcc tccgcctcca cctccacctc cacctccgcc cgccgcagta
     7801 cccgtcaagg tcgtaaaagt gatcccgcgg cagttcgacg ccggccgcct gatggcgccg
     7861 aaaacgatcc cgaaggaaat cgccatgatt aaggaagagg aattgcctcc tccgacgtcc
     7921 ggggccgtag gtgtgatcgg cggcgttcca ggtggggtgc ccggtgggac gccgggcggc
     7981 gtcattggcg gcatcatcgg tgctgttccg accgccgctc ccccgcctcc tcctccagtg
     8041 aaggaagcgc cgaagccggt cgttccacag cggattcgcg tgggcggcaa cgtgcagcag
     8101 gcaaagttag tgcgccaacc gaggccggtc tatccgccgt tggcgcggca agctcgcatc
     8161 cagggcacag tccggttgac cgccgtgatc gcgaaggacg ggacgattca ggagttgcag
     8221 gtggtcaatg gccacccgct tctggtccca tcggccctcg aagcggttaa acagtgggtc
     8281 taccagccga ccctcctcaa cggagagccg gtggaagtca tcacgcagat cgacgtgaat
     8341 ttcacgttga gccagtagtt ttgatgtcgt aaagacaaat tcaactcgca ggagatacag
     8401 acaatgcttt tgcaaatgca ggtttgggcg ttcctccttc ttcaggaggg cgccgtaggt
     8461 tgggacatca ggtccctttg ggcacagatg ggcatccccg cgaaactcgt ggttgtcgtt
     8521 atgttcctga tgtccgcgtg gtcgattgga gtgatgttgg atcggatgct ggcgtttagc
     8581 ggcgcccgca agcagtcccg gcaattcgct cccgccgttg ccggcgcttt gcgcgaaggc
     8641 aagctggatg aggcgatcaa gattgccgac cggtacaaga aaagtcactt ggcgaaggta
     8701 gttgtagccg gactccagga gtttcgggcg cacgaactga gtcacgacat tccaggagag
     8761 gatatcgaag cctctaagcg ggctctcgag cgcgcggaag cgatcgttca tgccgagctg
     8821 aagcgaggca cggccggtct ggcgacgatc gggtcgacgg ctccgtttgt cggactgttc
     8881 ggcacggtag tcggcatcat caacgcgttc aagggcattt cgaccgagaa gtcgactggt
     8941 ctgggcgcgg tggccggcgg catttccgaa gccttggtgg ctaccgctat cggccttttc
     9001 gtggccatcc ccgcggtgtg gatgtacaac tacttcgcgg gcaaggtcga agggttcgac
     9061 gtcgagatga gcaactccag ttccgagctg attgactact tcctgaagcg gtcccagcag
     9121 cggtccggag ccagcaaata gaatccggct gttcgcgctg gacataacag acctcgcgag
     9181 ttgaatgcgg gagacggcgg cgcggcgcct gtaagcgccc gccgtcgccc tggagagaac
     9241 ccgcggggag ttgggaggaa atcgaaatgg cgaaacccaa agcaacgccc ccggtatccg
     9301 acatcaatgt gaccccgatg gtcgacgtga tgctcgtgct gctgattatc ttcatggtag
     9361 tcacgccgat gttgtccaag ggtgtcagcg tcgaaatggt caggaccaag aaccccatcg
     9421 cgatgcaggc agcagataaa gaagatgcgg tcctggtcgc cctcacgcgc gaagggcgtg
     9481 tattcctagg cacgacgcag atgaaggccg atgaactggc gccgaaggtg aaggacctgc
     9541 tcgtcaacaa gctggacaag acggtgtaca ttcgcagtga tgccagggcg aagtacgaga
     9601 aagtcgttga ggtcgtggac aatctgcgcg cggctggcgt cgaccaggtt gggctattga
     9661 ccgaactgat ccaggagaag aagggcggcg ccgccgcagc ggcggccgca ccgcctgccg
     9721 gctagccggc atcggtggca agagaaagga gcaacaacca tggcaatggc agtgggcggc
     9781 cccggcggcg gcccgaaatc cgatatcaac atgacgccgc tgatcgacgt ccttctggtg
     9841 ctgctgatca tctttatggt gatcacgcct ctcactccga ggggcctcga tgcgctggtt
     9901 ccccagccac ctccgccggg tcagcaggca gacgagtccc ggcagcgtac tgtggtcatc
     9961 gtcatcaacc gggaccgtag tatgatgatc aacgctgagc cgaccgatga gggcagactg
    10021 ggcccgcggc tggaggaaat cttcaagacc cgagccgagc gtgtcgtctt cgtgaagggt
    10081 gacccggacc ttgagttcca gttcgtcgcc cgggcgatcg acatcgccaa aggtgccggg
    10141 atcgacaaaa tcggtctgat gactgctaag gtggaggccg gtctctgatc cggcctttcc
    10201 cgaggtcact cggactttaa tcaggagcat gctatgaatc gcaaagtcat tactgtactg
    10261 ggcgtcatcc taacgctcct cctggtggta gccggaacgg gttgccagaa gctcagggcc
    10321 cgcgaccagc tcaacaaggg ggttaatgcc ttccgtaacg cctcgtatcc cgaagcggtc
    10381 gaattcttca agcaagctgt ggaactcgat ccgcagttcc ccacggctcg gctgtatctt
    10441 gcgactgctt acatgatgca atacatcccg ggcgcagagt ccgacgagaa catgcggatg
    10501 gctcaggcgg cgcacgacca ctttgtccag gttttgcaaa cagacccgaa gaatacggtt
    10561 gcgcttgctt cgattgcttt gctttacttc aaccagaaga agctggacga agcccaggag
    10621 tggtatcaga aactgatcga agccgatccg aagtacaagg aggcgcatta caccctcggc
    10681 gtaatcgcct ggaccaagac cttccaaccg aggatggaag cccgtgcgaa gctcgggatg
    10741 agacctgagg atcccgggcc aatcaaggac aagaaagtcc gtgaggcgct cgccgaaaag
    10801 aacctaccag ttgtcgagtc cgggctggac catctgaaga aggccctcga gatcgacccg
    10861 gagtacgacg acgccatggc gtacatgaac ctcctctttc gtgagcgggc ggacctgcag
    10921 gagacgcagg gcgcttataa caaggacacc gaggaggcgg acaactgggt ccagaagacc
    10981 ctcgatacga agaagatcaa ggcatcgcgg acgcctcaag gcgggatcac cgcggaaggg
    11041 cagtagtttc tcttcttttc agcggctttc atcattccga tataattgaa gtagacgggc
    11101 ggcggacctc cctgcccgtc gtttcttcta tggcatcgga gccgaccacg gccatccccc
    11161 gcactccgga ggcgatcggc aacatgccgg ccgtcgatgg gcactacctc agtctgcgct
    11221 cctttcttca ggaacttcgt ccgaaggaag acttcactcc tctcgaccag gcgtaccggt
    11281 ttgccgcgaa gcaccatggc gcacagacca ggacctcggg tgagccgtac atggtccatc
    11341 cgcttgaggt gacccggata ctggcagaca tgaacatgga catgctctgt ctccaaacgg
    11401 ggctgttgca cgacgtggtc gaagacacag gcaccggtct cgaggagatt cgcagagtgt
    11461 tcggcgagga ggtagcgcac tgcgtagacg gtgtcaccaa gctgagcaaa ctcagcgtct
    11521 actccgctga agaccgtcaa gccgagagtg ttcgcaagat gctcctggcg atggtcaacg
    11581 acattcgcgt catcctcgtc aaactggcgg accgtgtcca taatatgcgt accctcgact
    11641 gcctctcgcg cgagcggcaa gagaggatcg cccgggaaac gctcgaaatc tatgcgccca
    11701 tcgctcaccg cctcggaatg gggaagatcc gcggcgaact cgaggatctg tcctttcatt
    11761 atttggagcc cgagtcggcc ggagaacttg cccggctgat tgaaagcaaa cgccaagaga
    11821 acgaggccta cctgcaacag attaaagggg ccatcgaggc aaaattgagg gcggagggga
    11881 tcccggcccg agtcgaaggc cgcctcaagc gggcctattc cgtctatcag aagatgaagc
    11941 gccagaagat cgcggtcgac caagtatatg atctgatggc acttcgtatt gttacggact
    12001 cggtcaagaa ttgctatgcc gcactcggcg tgatccacca cgagtggcat ccgatccccg
    12061 ggcgcatcaa ggatttcatc gccattccga ggcccaacct gtaccagtcg ctacatacct
    12121 ccgtagtcgg gcccgagggg cgcacgttcg aggtgcagat ccgcaccgag gaaatgcatc
    12181 gcatcgcaga ggaggggatc gcagctcact ggaaatacaa agagggaaac cggggccagt
    12241 ccgacgaaat ccagaggatc gcgtggctgc gccagcttgt cgagtggcag caggagatgc
    12301 gggatccgag tgagttcatg tccaccttga aggtggactt gtacccggag gaggtatact
    12361 gttttacccc gcgcggcaag gtgattgcgc tcccgcgagg cgctaccccg atcgactttg
    12421 cgtactccat ccactcggat gtcgggcaca cctgtgtcgg agccaaggtc aacggccgca
    12481 tcgttacgct gaaatacaat ctccgaaacg gggacgtagt ggagattctc acccagccag
    12541 gccacgttcc aagcaaggac tggctggcgt tggtgaaaac ctcgcgcgcc cgcaacaaaa
    12601 tcaagcacgt cattaacgtc accgagcgga taaaggcgat cgaaataggc gagaagttgt
    12661 ttgagaagga ggcgcgccgg ctcggtattt cactgaagag aatcgctagg gaagacctgg
    12721 accgagtggc cggcgagtac ggttgcagca agatggaaga cgtgcacgcg gcgctcggct
    12781 tcggccgctt ttcagcgcgc caggtgctcc agaagctggt cccccaggcg accgcggaaa
    12841 cccagcccgg cgaacaggca aaggcgccgc ctccgggtcc catacagccg tctcttgtcg
    12901 acgccaccga cctggtcatc aaggtccgcg gcatcgacga cctcatggtc taccgcgcca
    12961 agtgctgcaa cccgattctc ggcgaggcaa tcgtgggcta catcacccgg ggcaaaggtg
    13021 tcgctgtgca ttccagcagt tgcccgaatg tgcagaacct gttgtacgag gtggagcgga
    13081 agatcgatgt cgagtgggcg cgtagcggcg ccgaaccgtt ccccgtgaag atgatcatcc
    13141 acactgacga ccggcccgga atgttgcacc agttgacgtc aattctgtcg gacgaagaga
    13201 gcaatattcg cacgctggaa gcgcggatgg acgaccaaca cggcggcgat ggcgccctga
    13261 tcgacatgac cgtcgacgtg agagacaaga aacagcttga gcggattgta acctcaatgc
    13321 ggcgtctctc gggtgttcgt gacgtggagc ggatatgatg cctgccagcc acggcgcaac
    13381 cgaatttgtc cctctggaac cggagcacat cgccgtctcg aagtcgaaag ggatcaagat
    13441 cgactggaaa gactctcacc gcagtgaata cagtctccgg ttcctacgag accactgtcc
    13501 gtgcgccacc tgctccggag cgcacgggac agagcccatg aagcccgagt ctgcatctcc
    13561 gttccagatg tacaagccaa cgctgaagat gcttggagtc gagcctgtgg gtaactacgc
    13621 gatccgcatt cagtggaatg acggccacga cactgggatc ttttcgtggg agtatcttcg
    13681 cagaatctgc ggctgcccgg aatgcaccgg agaagcaggg tagggccccg aattacagca
    13741 ggcggaaatt aacctcgatg gttgccgcaa ccgtgacggg ttttccgtcc ttcgcaccgg
    13801 ggcggaactt ccacttcttg accgcctcga ttgccttctc atccaagcca agtccaagag
    13861 cccgaaggac tttgacgttc tggcaggtgc cgcgctcggt cacctcacag tagagcacaa
    13921 cggttccctg gaacttcgcc ttgcgggctt cctcggaata ctcaggctca accttaaaga
    13981 ccaacgtcgg agcgctgacg ccgccgccta cccggaacac gcccccaccg aagccaccgc
    14041 cctcgccggg accgtaaccc ggaccgcgtc cagggcctac gccgccgccc gaaccggaac
    14101 caatcccgcc gccggagcca ggtccgttgg acggggggcc gattttgccg agagggtcgc
    14161 cgtactgcgc catgttgacg ttgggcagat taacatccgg gggcaccacg atcgtaggct
    14221 ccatgatgag cttcggattt ggattgttga ccacggccat tgggggtgtg aactgcctcg
    14281 gggcgagttt cggaaggcgg cctttgctgg ccggtaaagg cgagcggtca ccgccaccgc
    14341 ctccaccgcc catcggctgc ggtttcgggg tctgtgggat gtacggcgcg atatccggcg
    14401 ccaccaggct gatcgcggtt tcctttacct tattccggat ggtgtcattg gccgctagtg
    14461 agaaggcaag agtcacaacg gagacatgaa tcaaaaccga gccgattccg gccctggcct
    14521 tatagccgcc gtagaatccc cagatgtcct tcactgcgac cggcttggag gtcaacttca
    14581 gcggcggcag tttctccgga aagatgagat ctttcaggtt ccgaacaact gagacatacc
    14641 acggcgttgc ggtttcgaga ggcagcaaat gtttcaggtg gtcgtccgct gttaagacgg
    14701 gcaagccatc ctgtgtatcg ccaacccgga cttcgtcgtt tttttcttgc tctgccatgt
    14761 cactgccagc tctcttccag cccctcccag acagaagaga aacgcttcca cgctcccctt
    14821 tcatggacgg agcaggcacc catgtcgttg catagagctc tcccttccat gcaacactac
    14881 cctacggaaa attgtagcat acactagccg caagagatcc actctccaca aaacaataga
    14941 cgattttaca aggcagttgg ttccgaggag ttgctgtccg gctcgagggg gcccgaagcc
    15001 tccagatcct ggatcatccg ccggatttcg aggaaacccc gccacccgac gaccacaacc
    15061 acaaccgcgt accaggcgac gcacccccaa gtgagcaaaa accagaatgg tcgtgacggc
    15121 atcgacgctc ctcagcccgc cttccttctc ctgccggccc acgaaccggc gagcattccg
    15181 gctgctgcca aaccaaagga ggcaagtccg gccacctcta cgtgcctcgc cagccctgac
    15241 agcaggaaag ctatcggact gacagccccg ccgacaatgg cggcgatcgc gccggtcttg
    15301 ttggcgcctt cccagtagca gcaggcgacg agcagaaccg agatgctcga gaggtagatg
    15361 ttcgatgtga ttgcgagata atcccacgca cgcccaggaa tctcgtacca gagcccgtac
    15421 gcgagcagga acagcccgat gccgaggatg ataacccggt tcaccaacag cccgcctctt
    15481 tcggaaaagg gccttttctt cttgagtggc cggatcacgt cgttgtagag aatactgccc
    15541 caggagagca tgtagctcga gtcagtggac atctcagcag ccagcatggc ggccacaaca
    15601 agtccgagca gtccggcagg cagcaaccgt gtaaggtagg ccggcatggc atgaatcggg
    15661 ctgagggact cgcctcttgc tcccagcatc gccagcgccg ccattcccca gtacgccgga
    15721 atcagatacc ggcccacgaa gaagatactg gtccgggtgt acatccgctg cgcagtctga
    15781 gggtccttgg ccgagagcac ccgctggata taggtctgcc acgtcagcac gaccgcaagc
    15841 atgctcagcg cctgccaaag tacgtaaccg ggccccatcc cggggctggt gaacggattg
    15901 aacgcgccat cgccgcgctc cgcggccaca acggcgacga ccttctccca ccccgcatcc
    15961 gcggcgacaa ggatcgttac cagcaccaca ccaccgccga gaacgaggaa ttgcaggtag
    16021 tccgtcacca acaccgacag catccctccg agcactgtgt acacgaggac catcagtagc
    16081 aggccggtca tcgtgagctc caggtagcgc atcggtattc cggtgacgat cgtcagaaac
    16141 tcgcctccca cccgcaggaa gattcccatg ttcagaagac cgccaagcac catgaccagc
    16201 ccggcaagca agcgcacacg ggatccgaag cggcgctcga gcaattccgg aattgtgagc
    16261 gcgccggaag cccgcagtgg tttgatcaca aagccggtcc agccgacggc ggcgatagcg
    16321 atggtaagca gtatccctgg gatggcgccg gcgaacccgt tcttgtaacc gagctcggcc
    16381 gtgtacatcg cggtgacgat gccgaactcc gtggccgcca gcgaggcgat gcccagcgat
    16441 acatccatct cgcggccggc gacgaggaaa tgttcgacct tgccgacgta gcgccgcatc
    16501 gccagcccgg tggccatcgt tccggcgagg tagatcgcga tgatcgacca gtccaatagt
    16561 ccgagggcgc tcgtcattgg agttgtgtgg gtttgccccc agcctgtctc atagaatctc
    16621 tcgaaacatc gcctcgaagt atgataatgc gtaccgagga aaagtacatg gtagccagca
    16681 aacatccagt ttccagttct catccgaaac gccgcgagtt tctgttcgcc gcgggcgcgt
    16741 cgccgctcgc cgcgggtgct gtagccgtgc aaggcagtgc tccggccggt ggcggaaagc
    16801 ccatcggagt cggtttgatc ggctgcggcg gccggggttc acacctgatt cagaccgccc
    16861 gccaactgaa tgaagtcggc gttccggcgg aagtcgttgc ggtgtgcgac atctaccagc
    16921 cgaggctgga gcgccttgcg acgcgtatga aagcaaagga atacagccgc tccgctgaac
    16981 tcattcgcga tccgcgagtg aacgcggtca tcgtcgccac gccagatcgg ctgcacgtcc
    17041 accatgcgct cgaagccgta cgcgccggca aagatgtcta ttgtgagaag ccgctcacgc
    17101 actgggctca gttcgacaaa cttaaggaac tggtgcgcga agtgcgggcg cggaaaacgg
    17161 tcttccaggt cggcgcgcag tgggtctccg acccgatgtg ggcgcgcgcg gctgagcaga
    17221 tcaggggcgg cgtcatcggc aaagttgtcc atgcgcagac cggctacttc cgccacggcg
    17281 acagcggcga gcgcggcatg ccgattgacg accccaacgc gcaggcgggc cacgggctcg
    17341 actgggaggc cttccaggcc gatgcgccgc gccgcgactt caccgtgtcg cgtttcttcc
    17401 agtggcgcat gtacgccgac tattccggtg gtcccgtgac ggatctttat ccgcacccgc
    17461 tgacccggct actgaaggct ctgggagtgg ggatgccgaa gaaggtcgtc gccttcggcg
    17521 gcaagttctt ctacgatggc ggccgcgatg ttccggacac gttcgacatg ctcattgagt
    17581 acccccaggg tatgacggtg tcgctactcg gcacgattgt gaatgacacc atgatcgaga
    17641 ctgtcatccg tggctcagag ggtaccgtga aattctcggg cgaggcgcaa ctgtccgtgt
    17701 ccccgcagcc cggcgctccg gaacgccggc gcacgatcgc gacggatcgc accggacttg
    17761 accacatgcg cgatttcatc gagtgtgtcc gaacgcgcca gcgacccatg tgcgacatcg
    17821 agctcggcta cgccgtgcag acggcgctga tcatggggat gcgttcgtac gtggaaggaa
    17881 gagtggcgtc cttcgacgcc gacaaagagg agatcagaat gtcatgatta cccgccgcac
    17941 ttttctcgtc gggggagctt tgttccccct cacgaatgcc gccgtccgca gtgaccacgt
    18001 caagggtgag aaagtctcat tccaccagga cgctcggctt cttttcgagt accgatattc
    18061 cgcagaagcc ccaaagacct atgttcaccc gttctgcgcg ccagacggga acgtcaccct
    18121 cgacagcccg gcggaccacg tacatcatcg cggggtgatg ctggcctgga gcgatgtcaa
    18181 cggcttcgac ttttggggag agcagaatcc gggcaagcac ggccggatcg tccaccggcg
    18241 gttcgcggga cttcgcaagg acaggcttgt ctcactcgaa gactgggtag ccgaaggcaa
    18301 aacgctcctc accgagcggc gcaccattgc cgtgcccatc tcttccaaag atgcagtgtg
    18361 gctcgactgg acgagcgagc tcagcgccgt ttccggaccg gtccagctct cggcggcg
//
LOCUS       DATIFQ010000303        20574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1438668, whole genome shotgun sequence.
ACCESSION   DATIFQ010000303 DATIFQ010000000
VERSION     DATIFQ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 20574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20574
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438668"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="VLE22_14225"
     CDS             <1..1707
                     /locus_tag="VLE22_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HSB15611.1"
                     /translation="IMKELMENQNAIIFIDELHTLVGAGSAEGSLDAANILKPALSRG
                     EIQCIGATTPAEYRKSIEKDRSLERRFQAVKVPPPTEVDAIKILFGIKERYEKFHAVA
                     YTDDAIETAVLASTRFIPDRFLPDKAIDLIDEAGARVKLRQTTLPSEVSEIQKRIKFI
                     VHRMENAIANHEFEKARFYSDEERKERENLRLLREKYNLDDTSTGVVTKDDIEDVVAR
                     WTGVPMTAIKEEEVAKLLRIEEELHKRVISQEKAIGALARAIRRSRAGLKSPKRPAGS
                     FLFLGPTGVGKTEVARALAEFLFGSEKSLVRFDMSEFMEKHSVSKLIGSPPGYVGYEE
                     GGQLTEKVKRSPYSVILLDEIEKAHPDVYNILLQVFEDGQLTDGLGNTVDFKNTIIIM
                     TSNLGARHLEKRVQIGFSAPTGEGGIPQKIEDMVMAEVKRAFNPEFLNRLDDVILFNS
                     LADEDLVKIIDLLVGQINVNLEPKQIKIHLAPEASKYILEKTCTDRSYGARPLRRALQ
                     KYIEDPLSEALIQGSLPRPAELEVYLSETGLYCRPVKEAEEQLVGAGGDSAPVGTPLY
                     VF"
     gene            complement(2298..3176)
                     /locus_tag="VLE22_14230"
     CDS             complement(2298..3176)
                     /locus_tag="VLE22_14230"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HSB15612.1"
                     /translation="MHKSAFVFLLSAALVSLAGCDFEEWGNAQRFQEDFSQSHPLKAG
                     GRLYVETFNGSVEISGWDRETAEISGTKYASTEQARNALRVDIVATGDSVRIRTVRPT
                     GHRGNMGAKYVIRVPNRTNLERVETSNGAVRIDGIEGDARVRTSNGSVHAERLKGALE
                     ATTSNAGVELDEVQGAAVVETSNGGIQASLREVTPRQPIKLTTSNGGIELEMGSFKEN
                     EIEAATSNGGITLRLPATTAARLKANTSNASISSDLEVKSAGEPGKNHLDGTIGAGGP
                     PITLTTSNGAIRIQKM"
     gene            complement(3433..3798)
                     /locus_tag="VLE22_14235"
     CDS             complement(3433..3798)
                     /locus_tag="VLE22_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15613.1"
                     /translation="MKFYWLFLGILAVWRVTHLLAAEDGPWDAIVRLRRLAGVGFWGN
                     LLDCFYCLSLWLAAPLALLLGEGWGERLLLWPALSGGAILFERLIPSAPKALWTEDPE
                     ERHDALLRPKETASRYRAN"
     gene            complement(3795..5981)
                     /locus_tag="VLE22_14240"
     CDS             complement(3795..5981)
                     /locus_tag="VLE22_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15614.1"
                     /translation="MPITMQGSWTVSVKSKSAAFPQRFVIEGAATGNGTYPGDVAAAP
                     VFVTGASWIVRIQNNPGTGFVDSADRIKFPAVVAGQYRFDIESDDGGGSDIDFNDLVL
                     TCSTPVTATDFLVYGNVLAYSGCLINPCIRRWIVIETADALIDALKNPVLRVPIERLY
                     PERIPLPPVPPGPPPDPPPFTPLVIPLAEEVAIPARRVQIVPRAPAVREVGAVESPEA
                     EEEVRSFAVRSVAVSAPARLAVDFDRVAAGRIADAIRPLCLTDPLAGYVLRFQEYDRT
                     AAELAGGPYTGTGSRETLGACATDRNGNYIFRFSRSLAQFFGEADVDVAAGEDEVVQA
                     MPDLIVQLLDATLPGGVAFETAPYWNVPLLKRIDLCIPRGRLRRPPTGCQEDALIYSI
                     GNIRIRVAATTFDAAGRVTCTDVSLPDIPQARCAAWGGALRFFACFNKDLPVTQYTVR
                     HRHRVGLGWSAWEFYQEPLPLLKITMVTEQIGPFDRMLELTSGGPLQPAKAYNNIQNN
                     LNFAGSERELRAVVSSAGGSPPYAPAPGTVQFRIQGYDASGMQVAGAVDTVTLYIDNT
                     IPELAIPSVTMGSQTGGDCALFSLSGEPSPARVTVRFKALQRQGFLRSYDLTVRKGNI
                     GGFGIATTTGPGGETSAPISSSYVHGGGASCNQLFGTLFPHEPLADAGGFVTAYVVPA
                     SGNWLAPGQPFCTFAFNVGAVLRVTNGYNTAEYSYGPVQYLLGIQQ"
     gene            6351..9788
                     /locus_tag="VLE22_14245"
     CDS             6351..9788
                     /locus_tag="VLE22_14245"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HSB15615.1"
                     /translation="MKKLLALNRSEIAIRILRAANELGLQTVAIYSKEDRLSLHRFKA
                     DEAYLVGPNKGPVEAYLDVDGIVGLAKEKGVDAIHPGYGFLSENPALARACDQAGIAF
                     IGPSAELLDLLGDKTAARRLAQRVGIPVVPGTEKPVSDPARAEKTAQEIGFPLIIKAA
                     FGGGGRGMRVVNSPEEFAPRLAEARQEAAAAFGNPAVFLERYIPRAKHLEVQILGDRH
                     GNVLHLYERDCSVQRRHQKVVEAAPAVSLDISIRKALADAAVQLAREAGYYNAGTVEF
                     LVDVDTGEWFFIEVNPRIQVEHTVTEMVTGIDIVRCQIQLAQGLSLHGPEMNLPPQEQ
                     VPQHGYALQCRVTTEDPEHNFVPDYGKLSTYRSPAGFGIRLDGGTAYGGAVITPYYDS
                     LLVKVTAWGREFVHACQRMDRCLREFRIRGVKTNIQFLENVVNHQVFQSGQATTSFLD
                     ETPGLFRFTPRKDRATKLLTYLGEVLVNGNPEVAGKKRPDRIVPAPAPAHEVSTPPKG
                     TRQLLNELGADKFCEWTRAQKRLLMTDTTFRDAHQSLVATRFRTYDMAAIADYVAHRL
                     HGLYSLEMWGGATFDVAMRFLHEDPWVRLRRLRELIPNICFQMLLRASNAVGYTAYPD
                     NAVRAFIYEAAAQGIDIFRIFDSLNWLPNMKVSMEAARKTGKVCEAAICYTGDILDPA
                     REKYSLGYYIRLAKELESMGAHMIAIKDMAGLCKPYAAGKLVKALRDVTDLPVHFHTH
                     DTSGVSASSVLKASDAGVDVADAAIASVSGSTSQPNLNSIVAAVAHTRRDTGIDIEAL
                     NRCADYWEVVRTYYAPFDTGPRTGSAEVYLHEMPGGQYTNLKEQAEAMGLGERWPEVV
                     RTYAEVNMAFGDIVKVTPSSKVVGDMALFLVSHGMSVREFERLGPDHHLTIPNSVVEM
                     FSGALGEPDGGWPKKIQKVILRGAKPQRGRPGARLKPVDLDETASMLEKKLGRKPARD
                     EVLSYIMYPEVFLKFARTQQTYGDMESLPTPQFFYGIEKGEEITVDLEPGKTLVIKFL
                     TIGEPHPDGLRTVFFELNGQPREVNIRDRSLQVKAPEQPKADPGIVGHVGAPIPGVVT
                     AVAVELNEEVKKGDKLLVMEAMKMQTTVYAPVAGRVMQKLVQPGQQVESKDLLIVIE"
     gene            complement(9816..10517)
                     /locus_tag="VLE22_14250"
     CDS             complement(9816..10517)
                     /locus_tag="VLE22_14250"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSB15616.1"
                     /translation="MASRRSSPADALVLFDIDGTLIRRAGPHHRESLIDAVRHATGLA
                     TTTDGIPLHGMLDPDILTVMLSNAGASRRLIREALPEILRRAQSLYVRRVPSLERKTC
                     PGVRRLLSGLSRRGVPMGLVTGNLPRIGWKKVERAGLKQHFTLAAFAGMHATRTGLAR
                     IAIRLAREKDLIRNGARISLIGDTPADVEAAKTNGIQAVAVATGLSSLEELSGASPDV
                     LLPDLRSLRLKDLLP"
     gene            10468..12324
                     /locus_tag="VLE22_14255"
     CDS             10468..12324
                     /locus_tag="VLE22_14255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB15617.1"
                     /translation="MSNRTRASAGELLREAILYYYSGMEKRSPAPRWLPVRSALSVAG
                     LIMLPLIVVVLAVLQYRWIGQASEADRERMQIGLNTSVSQFLEDYRSELYRVCTAFRP
                     EPGIVASRDWGQYVKRYDEWRRTTSFPDLAGDVFVWELEHIDGSRLIRVTMQSREVRA
                     VEWPPELGALRSTLDEQRRELGDSRRRDVRLRAWTLHEAAPALTHLLFQFTPPTTPGE
                     RPQFHVAGFLIVKLNPSCFEQRLFPELTRRHFGSRDGRAYQVAILNKETPAKPIYRSE
                     PAVSEAAAASAIVRVDLTGSVPENYILPRPALEQGRAEELGPPPVLVPGNEEESWQLL
                     VWHRFGSLDAVVARIRRRNLAVSFGVLLFLTAALTMLAISTRRAQRLAELQIDFVAGV
                     SHELRTPLAVICSAGDNLAEGVVDTKPQVQQYGELVRAEGRRLRAMVEQILHFAAGQT
                     DRRYELRPTEVAALVDGVLEDSGSAIQAAGFSVEAQIAPGLPRVLVDGAALKQCLQNL
                     VNNALKYGENARWIGVRANGNHGEVRITVEDRGMGIEPAELRHIFEPFYRGRAARAAQ
                     IHGTGLGLNLAARIAEAMGGRITVKSAPGKGSSFTLHLPAMKDDDRQKGEKV"
     gene            12326..13012
                     /locus_tag="VLE22_14260"
     CDS             12326..13012
                     /locus_tag="VLE22_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB15618.1"
                     /translation="MNENILLVEDEPGLRITLSDRLHSEGYQVETAADGEEGLERAAG
                     GPFDLIVLDIMLPRKSGLDVCRDLRQRGFTTPILMLTARGQTVDKVIGLKLGADDYLT
                     KPFEMPELLARIEALLRRARQAPAQPGSTHRFGSIQVDLRGTEVLRDGKQVPLSAKEF
                     QLLRYFIEHPGETLSREKLLSDVWGYGETMSTRTVDVHVAWLRQKLENDPKQPQLIVT
                     VHGLGYKFAG"
     gene            13152..14309
                     /locus_tag="VLE22_14265"
     CDS             13152..14309
                     /locus_tag="VLE22_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15619.1"
                     /translation="MKKRLWIPIVILAATVTTSGQTARPIGVVTAIDAGASSITLHTD
                     SGPDVLVRFDKQTAFLRVAPGERNLNNAVKIAITDVGVGDRILVRGTTSADSKSVAAS
                     SVIVMSKADIDKKHAADRAEWKRRGIGGIVTALRPEAKELTISVPGLMGSKITIITPV
                     GNAVWRRYAPDSVKFSDARPSRFEEIGVGDQVKAMGTRSDDGTRFTAEEIVSGSFLNI
                     AATVDEVNPGGSMVKVSDLATKKHLTVRVAPESNLRMVPPFLARMLAMRSAGEAGTEG
                     RLPPEGGRPGFSERPPEGVRPGMNSPAGTPDRPRDLESMIERMPAVTLADLKPGAAIA
                     IASTRGADPSQVTAITILSGIEPLLTATPQGGRQMLNGSWNLNLNMNMDQP"
     gene            14340..17255
                     /locus_tag="VLE22_14270"
     CDS             14340..17255
                     /locus_tag="VLE22_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15620.1"
                     /translation="MKLNKRLIDNIPALAAVCLIFLSAVQLAAQAASLNGRVTDPSGA
                     AVPGAMVTATGDGGAVKVATTGVDGRYAITNLPAGKYTVRATAQGFALHENTDVRVAG
                     STARTLDIRLELSVTKQEITVSDRAQVDVDPSSNVGAIVLKGEDLDALSDNPDDLESD
                     LQALAGPSVGPNGGQIYIDGFTGGNLPPKSAIREVRINQNPFSAEFDKLGFGRIEVFT
                     KPGSDKYHGQVFFNFGDSIFNSRNPFSIEKPSYQTKMFDGSLGGPITKKSSFFVNFDR
                     HAMGEAAVVSALTLDPSLNVTPFSQTVLNPRTRTSFSPRIDYQLSPNNTLVTRYSYTR
                     GSMENEGIGELSLPSRAFDSENTEHSVQVTETAVLGARVVNETRFQFLRTRSDQTALS
                     ADPSISVLGAFLGGGAGVGNSYTNEDRYELQNSTAMTAGTHTLKFGGRLRGSSLSDSS
                     MQNYNGTFTFTSLNAYRITLLGLQNSLTPAEIRAAGGGPSQFSIFGGRPLAGVDQFDA
                     GLFVQDDWRLHPNFSLNLGLRYETQNNISDHKDVAPRVGFAWGIGGKGRQPKTVIRGG
                     FGIFYDRIGEQLTLQAIRLNGVNQQQFLVPYPEFYPNVPSVAELAANRLPSAIREMDA
                     GLRSPYVAQTAISVERQLPKNVSLSLTYAHSRGLNVLRSRNINAPLPGTYDPLDPASA
                     VRPYGNAGDIYLYESSGMFKQNQLITNVNARVSPKLTLFGFYVFGKAESNADGAASFP
                     ANQYDLSTEWGRAGFDVRHRTFIGGSIVAPHGLRFSPFITASSGRPFDITVGRDLNGD
                     SLFNDRPALATDLSRASVVRTAYGIFDTNPLPGQIIIPRNYGDSPGQFTINMRLSKTF
                     GFGGETARSADASQQGMPSGPRLGGPGGHGGGPRGGGGFRGGPGGIFGEASTGKRYNL
                     TFSVSARNLLNHVNLGSPIGNLSSPLFGTSNSLAGGFGPFSSTTANRRVELQLRFSF"
     gene            17312..17911
                     /locus_tag="VLE22_14275"
     CDS             17312..17911
                     /locus_tag="VLE22_14275"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HSB15621.1"
                     /translation="MARATILDYDSGMEKRFPNGNVLRLVTGDITSIPADAIVNAANS
                     ALAGGGGVDGAIHRAGGPSIMRELDRIRASAGGCPTGSAVATAAGALPARWVFHAVGP
                     VYRDGRRGEAELLASCYRTCLALAEERQAVSISFPSISTGAYGYPIEEAAPIALRKIS
                     AHLESGTGSVREALVVLFSKSDYEIYDRALAGLGGEQLE"
     gene            17919..18422
                     /locus_tag="VLE22_14280"
     CDS             17919..18422
                     /locus_tag="VLE22_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15622.1"
                     /translation="MQVLQAGNQRYLLLELETELVERIATQSGFACKIEDYPRSIVAE
                     LSMEDRKAPLLLFDAADPGNLGWFSRCQFYVDGQTGAVLQTPISLANLKDRGGRSVPN
                     SVRLQIAKELPESFRLPGKQPVTEQVVYSVLASLLQALLESGVGVCGGTVVRPLAGRT
                     EAIGPRS"
     gene            18552..>20574
                     /locus_tag="VLE22_14285"
     CDS             18552..>20574
                     /locus_tag="VLE22_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSB15623.1"
                     /translation="MTGRTISHYQILDKLGEGGMGVVYRARDTHLDRFVAIKVLPSEK
                     VADPERKRRFVQEAKAASALNHPNIITIYDIDQADGIDYIAMEYAAGKRLDRLIPRHG
                     MPLSEALKCGVQIADALAAAHGAGIVHRDLKPGNVMVTDKSQVKVLDFGLAKLTETGE
                     SGEDFETRTIRDEEGPRTGEGRILGTAAYMSPEQAQGKKVNARSDIFSFGSLLYEMVT
                     GRRAFQGDSVVSTLAAIIKENPTPPSQVVEGLPKEVERLISRCLRKDPERRFQHMVDV
                     KMALEELKEESDSGTLEPEGHAKAKLRRKLLWAAAAVGVAVLAMAGVWFLRSRTPTSE
                     APLAAVPLTSYPGTEGSPSFSPDGTEVAFQWCPEDPGKSCDIYVMQIGVEQPSRLTDT
                     PAIEFSPAWSPDGRTIAFIRQLSGAATRFALVLIPQRGGRERVLGEVDTSGPGDFVDG
                     PHLAWTPDSRWLVCPRPDAGKGRCSLYLYSVETGEKRKLTNPPAGVVGDTAPAFSPDG
                     RILAFARQLEGYFRSDSYLLHLAGGYRPQGEPERVRLDDPYRYGAAWTPDGNEIVFGT
                     GKGLWRMAASKTATPRRLPFGPDNAVAPAISRQGNRLAYSVERSDTNIWRIDLREPGG
                     KPGAPVRFIASTLSDWCPAYSPDGQRIAFVSNRSGTSAIWACDADGSNAM"
BASE COUNT         4073 a         5884 c         7025 g         3592 t
ORIGIN      
        1 atcatgaagg agctgatgga gaaccagaac gccatcatct tcatcgacga gctgcacacg
       61 ctggtaggcg cgggatcggc cgagggatcg ctcgacgcgg caaatatcct gaagccggcg
      121 ctttcgcggg gcgagatcca gtgcatcggc gcgaccactc cggccgagta ccggaagtcg
      181 atcgagaaag accgctcgct tgagcgtcgc ttccaggcgg tgaaggtgcc gccacccaca
      241 gaggtggatg cgatcaagat cctgttcgga atcaaggagc gctacgagaa gttccacgcg
      301 gttgcctata ccgacgacgc gattgagacg gcagtgctgg cgtcgacgcg ctttattcca
      361 gaccgcttcc tgccggacaa ggcgatcgac ctgatcgacg aggcgggcgc gcgggtgaag
      421 ctgcggcaga cgacgctgcc ctccgaggtg tcggagatac agaagcggat caagttcatc
      481 gttcaccgga tggagaacgc gatcgcgaac cacgagttcg agaaggcgcg tttctactcc
      541 gacgaagagc gcaaggaacg cgagaacctg cggctgctcc gcgaaaagta caacctggac
      601 gatacgtcga ccggggtggt gacgaaggac gacatcgagg acgtggtggc gcggtggacg
      661 ggcgtgccga tgacggcgat caaggaggaa gaggtcgcca agctgctgcg gatcgaggag
      721 gagctgcaca agcgggtgat cagccaggaa aaggcgatcg gcgcgctggc gcgggcgatc
      781 cggcgttcgc gggccgggct gaagtcgccg aagcggccgg caggatcgtt cctgttcctc
      841 gggccgacgg gcgtcggcaa gacggaagtg gcgcgggcgc tggcggagtt cctgttcggg
      901 agcgagaagt cgctggtgcg gttcgacatg tcggagttca tggagaagca ctcggtctcg
      961 aagctgatcg gctcgccgcc gggatacgtg gggtacgaag agggaggcca actcacggaa
     1021 aaagtgaagc ggtcgccgta ttcggtgatc ctgctcgacg agatcgagaa ggcgcacccg
     1081 gacgtctaca acatcctgtt gcaggtgttc gaggacgggc agttgaccga cggcctcggc
     1141 aacacggtgg acttcaagaa caccatcatc atcatgacct cgaacctcgg ggcgcggcac
     1201 ctggagaagc gggtgcagat cggcttctcc gcgccgacag gcgagggcgg catcccgcag
     1261 aagatcgagg acatggtgat ggcggaggta aagagggcgt tcaatcccga attcctgaac
     1321 cgcctggacg acgtgatcct gttcaactcc ctggccgacg aagacctggt gaagatcatc
     1381 gacctgctgg tgggccagat caacgtgaac ctggagccga agcagatcaa gatccacctg
     1441 gcgccggagg cgtcgaagta cattctggag aagacctgca cggaccgcag ttatggggcg
     1501 cgtccgttgc ggcgggcgct ccagaagtac attgaggacc cgctctcgga ggcgctgatt
     1561 caggggtcgc tgccgcggcc ggcggagctg gaggtttacc tgagcgagac gggcctgtac
     1621 tgccgtccgg tgaaggaagc cgaggagcag cttgtcggcg cgggaggcga ttcggcgccg
     1681 gtgggcacgc cgctgtacgt gttctaagcc cggccaggga cgcattctcg aaaacaacgc
     1741 gttcgcgcgg cgcacgagtg aatgcgttga tttcaaatca cttgttgtga caagactcgg
     1801 tgcagggacg cattttccgc aagatgcgtc actggatttc atacttttgc cgatccggcc
     1861 ctagcccggt tccggaaatt gaccaaacaa agccaatttt cggataaatc tacccttttc
     1921 attccattac agagaaacat agttttccac agccccacga aagggtgagg gtattttcac
     1981 tcatatgggt atgaaatctc ggttcgcggg tacggggtac cagcggtcag ctatcggcga
     2041 tcagccgtca gccggtccca agggcacggg ggaccatctg actacccaat accacggggg
     2101 cgaagaagac cggcgtcggt tcgtgggtgt agttgttgaa ataaaaggaa atagcttgag
     2161 gcgaaggatg tgaatgccaa cggtggggcg gaaacggaga aattccggct gggcggcctc
     2221 ggatccagcc ggaaggagga aaatcagcaa tcagcggtca gcaatcagcg gtcagccatc
     2281 agcggtcagc gctgacgtca cattttctgg atgcggatgg cgccgttgga ggtggtcagt
     2341 gtgatgggag ggccgccggc gccgatagtt ccatcgaggt ggttcttgcc gggttctccg
     2401 gcgctcttga cttcaagatc gctggagatc gaggcgttcg aggtgttggc tttcaatcgc
     2461 gccgccgtgg tggcgggcag gcgcagggtg atgccgccgt tgctcgtagc cgcttcgatc
     2521 tcattctcct tgaacgatcc catctccagt tcgatgccgc cgttcgacgt ggtcagcttg
     2581 atgggctggc gtggcgtgac ttcgcgcagc gaggcctgga tgccgccgtt cgacgtctcg
     2641 acgaccgcag caccctgaac ctcgtcgagt tccacgcccg cgttggaggt ggtcgcctcc
     2701 agcgctccct tgaggcgctc ggcgtggacg gagccgttcg aggtgcggac gcgcgcgtct
     2761 ccctcaatcc cgtcgatccg cacggcgccg tttgacgtct cgacacgctc cagattggtc
     2821 cggttcggga cccggatcac atattttgcg cccatgttgc cgcggtgtcc ggtgggacgc
     2881 actgtgcgaa ttcgaacgga gtcgcccgtg gctacgatgt ccacgcgcaa cgcgttccgg
     2941 gcctgctcgg tactggcgta ctttgtgccg ctgatttcgg cggtctcccg gtcccagccg
     3001 gagatttcga cggagccgtt gaaggtctcg acgtagaggc ggccgcccgc cttcagggga
     3061 tggctttggc taaaatcctc ctgaaaccgc tgggcgttgc cccattcctc gaaatcacat
     3121 ccggcgaggc taaccaacgc cgccgagagc aggaatacaa atgctgactt gtgcatgttc
     3181 gcgtacctcg ctggagagat acgcaaccag cagatcggaa gttccgtcga gtgcgggccg
     3241 gctcagaggc ggcgaaggcg ggggacggcg gcgagtgagg gggcgtcacg gggatcgacc
     3301 gggacgacgg cgccggaaaa ggcaaaacgg tagggcgcac cggtgaccgg gccaactacg
     3361 gtaaggccgg tgcgtccggt gtactcgaaa tcgacaggct ccggcggcgg cggcgcgggg
     3421 cgggcagccg gatcagttgg ctcggtaacg agaagctgtc tcttttggcc gcagcaaggc
     3481 atcgtgacgt tcctcaggat cttcggtcca aagcgctttc ggagcgctcg ggatcaggcg
     3541 ctcgaacagg atggcgccgc cggacagggc cggccacaaa aggagccgct ccccccaacc
     3601 ctccccgagc aggagggcga gcggagcggc cagccagagg cttaggcagt agaagcagtc
     3661 cagcagattt ccccagaatc cgacgccggc gaggcgcctc agccgcacga tggcgtccca
     3721 cgggccgtct tctgccgcaa gcaggtgcgt gacgcgccag acggcgagga taccgaggaa
     3781 gagccagtag aacttcattg ctgaatgccg agcaggtact gcacagggcc gtaggagtac
     3841 tcggcggtgt tgtagccgtt ggtcacccgc aggacggcgc ctacgttgaa ggcgaatgta
     3901 caaaacggct gcccaggtgc gagccagtta ccgctggcag ggacgacata ggcggtaacg
     3961 aaaccgccgg cgtcggccag cggctcatgc gggaagagcg taccgaacaa ctggttgcag
     4021 gaggctcctc ccccgtggac gtaggaactg gaaatcggcg cgctcgtttc gccccccggc
     4081 ccggtagtgg tggcgatccc gaagccgccg atgttgccct tgcgcaccgt gaggtcgtag
     4141 gaccggagaa agccctgccg ctggagcgcc ttgaatcgca cggtgacgcg ggcgggggag
     4201 ggttcgccgg agaggctgaa gagcgcgcag tcgccgccgg tttgggagcc catggtgacg
     4261 gagggaatgg caagctccgg aatcgtgttg tcgatgtaga gggtgacggt gtccacggcg
     4321 ccggccacct gcatgccgga agcgtcgtaa ccctgaatcc ggaactggac ggttccgggc
     4381 gccggggcgt acggcggcga acctcctgcg gagctaacga cggcccggag ttcgcgttcg
     4441 gaaccggcga agttgagatt gttctggatg ttgttatacg ccttggccgg ttggagcggc
     4501 ccgcccgagg tcaactcgag catgcggtca aaaggcccga tttgctcggt gaccatggtg
     4561 atcttgagca gggggagcgg ctcctggtag aactcccatg cagaccaacc gaggcccacg
     4621 cggtggcggt gacggactgt gtattgggtg accggaaggt ccttgttgaa gcaggcgaag
     4681 aagcggagcg cgcctcccca ggcggcgcac cgtgcctgcg ggatgtcggg cagagagacg
     4741 tccgtgcagg tgacacggcc ggcggcgtca aacgtggtgg cggcgacgcg gattcggatg
     4801 ttcccgatgg agtagatgag cgcatcctcc tggcatccgg tgggcggacg gcgaaggcgg
     4861 ccgcggggga tgcagaggtc gatgcgtttc agcaacggaa cgttccagta gggcgcagtt
     4921 tcgaaagcca cgccgccggg caaggtagca tccaggagct ggacgatgag gtcgggcatc
     4981 gcctggacga cctcgtcctc tccggcggcg acatccacat cggcttcgcc gaaaaactgg
     5041 gcgagcgagc gggagaagcg gaagatatag tttccgttgc ggtcggtggc gcatgcgccg
     5101 agggtctcgc gagacccggt gcccgtgtag gggccaccgg ccagttcggc ggcagtacgg
     5161 tcgtactcct ggaagcggag cacgtagccg gcgagcggat cggtaagaca gagcggccgg
     5221 attgcatcgg cgattcggcc ggcggcgaca cgatcgaagt ccacggcgag acgcgcgggc
     5281 gccgaaacgg caacggagcg gaccgcgaat gaccgcactt cctcctcggc ctctggggat
     5341 tccaccgctc ctacttcgcg gaccgcgggc gcgcgcggga caatctggac gcgcctggcg
     5401 gggatggcga cctcctccgc gagagggatg acgaggggtg taaagggcgg cgggtcgggg
     5461 ggaggaccgg gcggaaccgg cgggagtgga atccgctccg gataaagtct ttcgatgggg
     5521 acgcggagga cggggttctt cagggcgtcg atgagggcgt cggcggtctc gatgacgatc
     5581 cagcgccgga tgcaggggtt gatgaggcag ccgctgtagg cgaggacatt tccgtaaacg
     5641 aggaagtcgg tggcggtaac gggggtggaa caagtgagga cgaggtcgtt gaagtcgatg
     5701 tcggaaccgc cgccgtcatc actctcgatg tcgaagcggt actgaccggc tacgaccgcc
     5761 gggaacttga tccggtcggc agagtcgaca aagccggttc cggggttgtt ctggatgcgg
     5821 acgatccagg aggcgccggt gacaaagacc ggggcggcag cgacgtcacc gggataggtt
     5881 ccgttgccgg tggcggcgcc ctcgatgacg aaacgttgcg gaaacgctgc actcttggac
     5941 ttgaccgaga cggtccagga gccttgcatg gtgatgggca tgttatttcc tccagtgtgt
     6001 gagcggcctg tgggtgctcc ttaagaaagt tcgctgtagc ttacgctcct taactgcatg
     6061 cgtccgctcc ctgcggccac ggctcggttt gcatgggaca agattcgaaa aaaccgggcg
     6121 tggcgcggcg ggaccacgct cacgcccggg gcgtcccagg agccgcggaa tcgatcgcgg
     6181 cagcggccct ccgtaatgga gcgtaaggga tgccgggggt tccgtcaatg tccattttgg
     6241 ccatctttcg gctagggact gctagcagat aactccgttt gttaacccgg gaataacgcc
     6301 ggcaaggcgc atgatattct gaaggttcac ccccttcatc tctggtattc atgaaaaaac
     6361 tactggcgct aaatcgcagc gagatcgcta tccgcatctt gcgggcggct aatgaacttg
     6421 ggctccaaac agtcgctatt tactcgaagg aagaccgact cagtctgcac cgcttcaagg
     6481 cggacgaagc gtacctcgtg ggtccgaaca aggggccggt ggaggcatac ctcgacgtcg
     6541 acggcatcgt cggattggcc aaggagaagg gcgttgatgc gatccacccg ggatacggct
     6601 tcctttctga gaacccagcg ctggcgcggg cctgcgacca ggcaggcatt gcattcatcg
     6661 ggccgagcgc cgagttgctc gacttgttgg gcgataagac ggcggcgcgg agactggcac
     6721 agcgagtggg cattccggta gtgccgggca cggaaaaacc ggtctcggat ccggcgaggg
     6781 ccgagaagac ggcgcaggag atcggattcc cgctgatcat caaagcggca ttcggcggcg
     6841 gcgggcgggg catgcgggtg gtgaattccc cagaggaatt cgcgccgcgc ctagccgagg
     6901 ctcggcagga agcggcggcc gcattcggta acccggcggt gtttctggaa cgctacatcc
     6961 cgcgggcgaa acatctggag gtgcagatcc tgggtgaccg gcacggcaac gtgttacacc
     7021 tctacgagcg cgactgctcg gtacagagga ggcaccagaa ggtggtggag gcggcgccgg
     7081 cagtctcgct ggacatctcg atccgtaagg cgttagcgga cgcggcggta caactggcgc
     7141 gggaggcagg ttactacaac gcgggaacgg tggagttcct ggtggatgtc gacaccggcg
     7201 agtggttctt catcgaggtg aatccgcgca tccaggtgga gcatacggtg acggagatgg
     7261 tgaccggaat cgacatcgtc cgttgccaga tccagctcgc gcaggggctt tcgctgcatg
     7321 gacccgaaat gaacctgccg cctcaggagc aggtgccgca gcacggctat gcgctgcagt
     7381 gccgcgtgac gaccgaggat ccggagcaca acttcgttcc cgactacggg aagctgtcga
     7441 cgtaccggtc gccggcggga ttcggcatcc ggctggacgg agggacggca tacggcggcg
     7501 cagtgatcac gccgtattac gactcgctgc tggtgaaagt gacggcatgg gggcgggagt
     7561 tcgtccatgc gtgccagcgg atggaccggt gcctgcggga gtttcgcatc cggggcgtca
     7621 agacgaacat tcagttcctg gagaacgtcg tcaaccatca ggtatttcag tccgggcagg
     7681 cgacgacgtc gttccttgac gagacgccgg gcctgttccg cttcacgccg cggaaggacc
     7741 gagcgacgaa gctgctgacc tacctgggcg aagtgctggt gaatgggaac cccgaggtag
     7801 cggggaaaaa gcggccggac cggatcgtac cggcgccggc gcctgcgcac gaagtcagca
     7861 cgccgccgaa gggaacgcgg caactgctga acgaactggg ggccgacaag ttctgcgaat
     7921 ggacccgagc gcagaagcgg ctgctgatga cggacacgac gttccgcgac gcgcaccagt
     7981 cgctggtggc gacgcgtttc cgcacgtacg acatggcggc gatcgccgac tacgtggcgc
     8041 accgcctgca cggtctctac agcctcgaga tgtggggcgg cgcgacgttc gacgtcgcca
     8101 tgcgctttct gcacgaggat ccgtgggtgc ggctgcggcg gttgcgggaa ctgattccga
     8161 acatctgctt ccagatgctg ttgcgagcgt cgaacgcggt gggatacacg gcgtatcccg
     8221 acaacgcagt gcgcgcgttc atctacgagg cggcggcgca ggggatcgac attttccgta
     8281 tcttcgattc gctgaactgg ctgccgaaca tgaaggtgtc gatggaggcg gcgcgtaaga
     8341 cgggcaaggt atgcgaagcg gcgatctgct acacgggcga catcctggac ccggcgcgcg
     8401 agaaatactc gctcggctac tacatacggc tggcaaagga actggagagc atgggcgcgc
     8461 acatgatcgc catcaaggac atggcagggc tgtgtaagcc gtatgcggcc ggaaagctgg
     8521 tgaaggcact gagggatgtc acggacctgc cggtccactt ccatacgcac gataccagcg
     8581 gcgtgagcgc ttcgagtgtg ctgaaggcga gcgacgccgg ggtggatgtg gcggacgcgg
     8641 cgatcgcgtc cgtgagcggg tcgacgagcc agccgaacct gaactcgatc gtcgcggcgg
     8701 tagcgcatac gcggcgcgac acggggatcg acatcgaagc gctgaaccgc tgcgccgatt
     8761 actgggaggt agtgcggacc tactacgctc cgtttgacac gggtccacga acgggatcgg
     8821 ccgaggtgta tctgcacgag atgcccgggg ggcagtacac caatctcaag gagcaggctg
     8881 aggcgatggg gctcggcgag cgctggccgg aagtcgtgcg cacatacgcc gaggtgaaca
     8941 tggccttcgg cgacatcgtg aaggtgacac cgtcaagcaa agtggtcggc gacatggcgc
     9001 tcttcctggt gagccacggc atgagcgtgc gggagtttga gaggctcggt ccggaccatc
     9061 acctgacgat tccgaattcg gtagtggaaa tgttttccgg ggcgctgggt gagccagacg
     9121 gcggctggcc taagaaaatc cagaaggtaa tcctgcgcgg ggcgaaacct caacggggcc
     9181 ggcccggagc gcggctgaag ccggtggatc tggacgagac cgcgtcgatg ctggaaaaga
     9241 aactcgggcg gaagccggcg cgggacgaag tgctgagcta catcatgtat ccggaggtgt
     9301 tcctgaagtt cgcccggacg cagcagacgt acggcgatat ggagagtcta ccaacgccgc
     9361 agttcttcta tggcatcgaa aagggcgagg agatcacggt ggacctggag ccaggcaaga
     9421 cactggtgat caagttcctt acgatcggag agccgcaccc cgatggactc cgtacggtgt
     9481 tcttcgaact caatggtcag ccgcgggaag tgaacatccg ggaccgttcg ctccaagtga
     9541 aggcgccaga gcagccgaag gcggacccgg gcatcgtggg acacgtcgga gcgcccatcc
     9601 cgggagtggt gacggcggtg gcggtggaac tgaacgagga agtgaagaag ggcgacaagc
     9661 tgctggtgat ggaagcaatg aagatgcaga ccaccgtgta tgcgcccgtt gccggtaggg
     9721 tcatgcagaa actggttcag ccaggccagc aggtggagtc gaaggacctg ttgatcgtca
     9781 ttgaatgacg cctgggagtg cgttgatcac ggcagtcacg gcaggagatc tttcagtcgt
     9841 aacgagcgaa gatcgggaag caggacgtcc ggcgaagcgc cgctaagctc ctcaagactg
     9901 gagagtccgg tagctactgc gaccgcctgg atgccgttgg ttttcgcggc ctcgacgtcg
     9961 gccggcgtgt caccgatcag agagatgcgg gcgccgttgc ggatgaggtc cttctcgcgg
    10021 gcgaggcgga ttgcgatgcg cgcgaggccg gttcgggttg catgcattcc ggcgaaggcg
    10081 gcaagggtga agtgctgctt caagccagcc cgctccactt tcttccaacc gatgcgggga
    10141 agattgcccg tgaccaggcc catcggaaca ccacggcggg acaggcccga gaggagcctt
    10201 cgcacaccgg gacatgtctt gcgctcaaga cttggaacgc ggcggacgta aagagactgc
    10261 gcacggcgca ggatttcagg cagagcctcg cggatgaggc ggcgggaagc gccggcgttg
    10321 ctcagcatga cggtgaggat gtccgggtcg agcatgccgt ggagcgggat gccgtcggtg
    10381 gtggttgcaa gtccggtggc gtgccgaacg gcgtcgatga gcgattcacg atgatgcggc
    10441 ccggcgcgcc ggatcagcgt accgtcgatg tcaaacagga cgagagcgtc cgccggagag
    10501 cttctgcggg aggccattct ctactactat agcggcatgg agaagcgctc cccggccccg
    10561 cggtggctgc ctgtgcgttc cgccctttca gtggccggcc tgatcatgct gccgctgatc
    10621 gtggtggtcc tggcggttct ccaatatcgc tggattgggc aggcgagcga ggccgaccgc
    10681 gaacggatgc agatcgggct gaacacttcg gtcagccagt tcctcgaaga ctaccgcagc
    10741 gaattgtacc gtgtctgcac cgccttccgg cccgagcctg ggatcgtcgc gagccgcgac
    10801 tggggtcagt acgtcaagcg ttacgacgag tggaggcgga cgacttcgtt ccccgacctg
    10861 gccggagacg tgtttgtgtg ggagctcgag catatcgacg gatcccgcct gattcgagtc
    10921 accatgcaga gtagagaagt gcgggcggtg gaatggccac ccgaacttgg agcgttacgt
    10981 agcacactgg acgagcaacg gcgcgaattg ggcgacagcc gccggcggga cgttcgcctg
    11041 cgtgcctgga cgttgcacga ggcggcgccc gcattgacac acctgctctt ccagttcaca
    11101 ccacccacca cgccgggtga gaggccgcag ttccacgtgg ctggattctt gatcgtaaaa
    11161 ctgaacccca gttgttttga gcaaaggctt ttcccggagc tgacgaggag gcattttggc
    11221 tcccgcgacg gacgcgcata ccaagttgcg attctgaaca aagaaactcc ggcgaaaccg
    11281 atctaccgtt ccgaaccggc cgttagcgaa gccgccgccg cttcagcaat cgtccgggtc
    11341 gatcttacgg gatctgtgcc ggagaactac atcttgcctc gacccgcgct ggagcaaggg
    11401 cgcgcagagg aactcgggcc tccgccggtg ctcgtgcccg ggaatgaaga agagagttgg
    11461 caactgctgg tgtggcatcg gttcggctcg ctcgatgcgg tcgtggcacg catccggagg
    11521 cgcaatctcg ccgtgagttt tggtgtcctc ttgttcttga ccgctgcttt gacgatgctg
    11581 gccatctcca cgcggcgggc tcaacggctg gccgaactcc agatcgactt cgtggcgggt
    11641 gtttcgcacg agcttcgcac gccgctggcg gtgatctgct ccgccggaga caacctcgcg
    11701 gaaggggtgg tggatacgaa gccgcaggtg cagcaatacg gggaactggt ccgggcggaa
    11761 gggcgccgcc tccgcgcgat ggtggagcag attctccatt ttgccgcagg acagaccgat
    11821 cgcaggtacg agctgcgccc cacagaggtg gccgcactgg tggacggcgt gcttgaggac
    11881 tcgggctccg cgattcaggc cgccggattc tccgttgagg cgcagatcgc accgggtctg
    11941 ccgcgggtcc tggtggacgg ggccgcattg aaacaatgtc tccagaacct cgtcaacaac
    12001 gccttgaaat atggtgagaa cgcacggtgg atcggcgtgc gcgcgaatgg taatcacggc
    12061 gaggttcgaa tcacggtcga ggacagaggc atgggaatcg agcctgcgga gttgcgtcac
    12121 atcttcgagc cgttctatcg cggcagggca gccagggcgg cacaaattca cgggaccggg
    12181 ctcggactga acctggcagc gaggatcgcc gaagcgatgg gtggccgaat cacggtgaaa
    12241 agcgcacccg gcaagggcag ctccttcact ctccatctgc ctgcgatgaa agacgatgac
    12301 cggcaaaagg gcgagaaggt gtgacatgaa cgagaacatc ctgctggtcg aggacgaacc
    12361 gggtctgcgc atcaccctca gtgaccggct ccacagcgaa ggctatcaag tggagacggc
    12421 cgccgatggg gaagagggcc tggagcgtgc cgcgggcggg cccttcgatc tgatcgttct
    12481 cgacattatg ctgccgcgaa agagcggctt ggatgtatgc cgggatttgc gccagcgtgg
    12541 attcacgaca ccgatcctta tgctgacggc gcgcggccag accgtcgaca aggtgatcgg
    12601 gctgaagctc ggggcggacg attacctgac caaaccgttc gagatgcctg aattgctggc
    12661 gcggatcgag gcgctgctcc ggcgcgcccg ccaggcgccg gcgcagcccg gatcgacgca
    12721 ccggttcggc tcgatccagg tcgatctgcg gggaacggaa gtcctgagag atggaaagca
    12781 agtgcctctg tcggccaagg agttccagtt gctccggtac tttatcgaac accccggcga
    12841 gacattgtcc cgcgagaaac tcctgagcga tgtctggggt tacggcgaaa ccatgtcgac
    12901 gcggacggtg gatgttcatg tcgcatggct ccggcagaag ctcgagaacg atccgaagca
    12961 gccccagttg atcgtgacgg ttcacggcct cggatataag ttcgccgggt gaaaagctcc
    13021 ggtaccgttc atcagtcctt cacgattgtt aagacgatgt aaagcgagtt ttacccggtc
    13081 tttaccgccc gagcataacc ctatcgcgtc caaagtcgtt tctacatgta aggggccgcg
    13141 ggccccggtc gatgaagaaa cgactttgga ttccaatagt tatcctggct gccacagtaa
    13201 caacgtccgg ccagacggcg cgcccgatcg gggtcgtgac agcgatcgac gccggcgcga
    13261 gttcgatcac tctccacacg gactccggac cggacgttct ggtccggttc gataaacaga
    13321 ccgccttcct tcgggtcgcg cccggtgaac ggaacttgaa caacgccgtg aagattgcga
    13381 ttaccgatgt cggcgtagga gaccgaattc tcgtgcgcgg aactacctca gccgattcga
    13441 agtcggtcgc cgcatcgtcc gtgattgtca tgagcaaggc tgacatcgac aagaagcacg
    13501 ccgccgatcg cgcggaatgg aagagacgag ggatcggcgg aatcgttacc gcgctgaggc
    13561 ctgaggccaa ggaactcacg atctccgtgc ccggcctgat gggttccaag atcacgatca
    13621 ttacgccagt gggaaacgcg gtgtggcgac ggtatgcgcc cgactcggtg aaattcagcg
    13681 atgcgaggcc gagccggttc gaggaaatcg gggtaggcga tcaggtgaag gcgatgggaa
    13741 ccaggagcga tgacgggaca cgattcaccg cggaggagat tgtctccggc tcattcctga
    13801 acatcgccgc gacggtggat gaggtgaatc caggcggcag catggtgaag gtgtcggacc
    13861 tggcaacaaa aaaacacttg acggtgagag tggcgccgga atcgaacttg cgcatggttc
    13921 cacccttcct agcgcgaatg ctggcaatga ggagtgctgg ggaggcgggt actgagggac
    13981 gactcccacc ggaaggaggg cgaccaggtt tttcggaaag accgccagag ggggtccggc
    14041 ccggaatgaa cagcccggca ggcacgcctg accgtccacg ggaccttgaa tcgatgatcg
    14101 agaggatgcc ggctgtgacg ctggccgact tgaaaccggg cgccgccatc gccatcgcga
    14161 gcacgcgcgg tgcggatcct tcacaagtga ctgcaattac gattctctcc ggtatcgagc
    14221 cgctgctgac ggcgacgcca cagggcggcc ggcagatgct gaatggctcg tggaatctca
    14281 acctgaacat gaacatggac cagccgtaaa gcggtgggtc cggagcattg agactgcgaa
    14341 tgaaactgaa caagcgactg attgacaaca ttccggcgct ggccgcggtg tgcctgatat
    14401 tcctctctgc agtgcagttg gcggcgcagg cggcatccct gaacggccgg gtgaccgacc
    14461 catccggcgc cgcggttcct ggcgcgatgg taaccgcgac cggcgacggc ggcgcggtaa
    14521 aagtggcgac aacgggtgtg gacggcagat acgccatcac gaaccttccg gcgggcaaat
    14581 acaccgttcg ggccacggca caaggattcg cgcttcacga gaacacggat gtgcgagttg
    14641 ccggatcaac ggcgcgcacg ctcgatatcc gtctggagct gagcgtaacg aagcaggaaa
    14701 tcaccgtgtc cgacagggcg caggtggacg tcgacccttc gagcaatgtg ggagcgattg
    14761 tgttgaaagg tgaagatctc gacgcgctct cggacaaccc ggatgatctc gaatcggatc
    14821 tccaggcgct ggcggggcca tcggtagggc cgaacggagg acaaatctac atcgacgggt
    14881 tcacgggcgg aaacctgcca ccgaagtccg caattcgaga agtccgcatc aaccagaatc
    14941 cgttttcggc cgagtttgac aagctcggtt tcggacgcat cgaggttttc acgaagccgg
    15001 gctcggacaa atatcacggg caggtcttct tcaacttcgg cgattccatt ttcaactcgc
    15061 ggaatccatt ctcgatcgag aagccgtcgt accagacgaa gatgtttgac ggcagccttg
    15121 gcggaccgat cacgaagaag tcctcgtttt tcgtaaattt cgaccgccat gcgatggggg
    15181 aagccgctgt agttagcgct cttaccctcg atccgtcact caacgtcacg ccgtttagcc
    15241 agacggtcct gaatccgcgt acgcggacct catttagccc gcggatcgac taccagttgt
    15301 cgcccaacaa caccctggtg actcggtaca gctatacgcg cggcagcatg gagaacgaag
    15361 gtatcgggga actctcgctg ccatcgagag cgttcgattc ggaaaatacc gaacattcgg
    15421 tgcaggtgac cgagacggcc gtgctgggtg ccagagtcgt taacgagacc cgcttccaat
    15481 ttctgagaac gcggagcgac cagaccgcgc tgagtgccga cccgtccatc agcgttctgg
    15541 gagcattcct gggtggaggc gcgggtgtcg gaaattcgta caccaatgaa gatcgctacg
    15601 agcttcagaa ttccacggcg atgacggccg gcactcacac gctgaagttc ggcggacgcc
    15661 tgaggggctc gtcgctctcc gactcctcca tgcagaacta caacggaacc ttcacgttca
    15721 cgtcactgaa cgcataccgg atcacgctgc ttggccttca gaacagcctt acgcccgcgg
    15781 agatccgcgc cgccggaggc ggcccaagcc agttctcgat tttcggaggc cgaccgctgg
    15841 ccggtgtcga ccagtttgac gctgggctgt tcgtccagga cgactggcgg ctgcacccca
    15901 acttctcgct caacctgggt ttacgctacg agacacagaa caacatcagc gaccacaagg
    15961 acgtcgctcc tcgtgtgggt ttcgcctggg ggatcggcgg caagggacgg cagcccaaga
    16021 cggtcatccg tggcggattc gggatctttt acgaccgaat cggcgagcaa ctgactcttc
    16081 aggcgatccg gctcaacggc gtcaaccagc agcaatttct ggtaccgtac ccggagttct
    16141 acccgaacgt tccttccgtc gccgaattgg ctgccaaccg gctaccatcg gcaatccgtg
    16201 aaatggatgc cgggttgcgg tccccatacg tcgcccagac ggccatcagc gtcgaacggc
    16261 agttgccaaa gaacgtgtcc ctgtccttga cgtacgcaca ttcccggggg ctcaacgtgt
    16321 tgcgctcgcg caatatcaat gcaccgctcc ccggcacgta cgaccccctg gacccggcga
    16381 gtgcagtgcg cccgtacggc aatgcgggag acatctacct gtacgaatcg agcggcatgt
    16441 tcaaacagaa ccagctcatc acaaacgtga acgcgcgggt gagtccgaag ctgaccctgt
    16501 tcggtttcta tgtgtttggc aaggcggaga gtaacgcgga cggcgcggcg agttttccag
    16561 caaaccagta cgacttgagc acggaatggg gccgcgcggg attcgatgtc cgccaccgca
    16621 cgttcatcgg aggctcaatc gttgcaccgc atggcctgcg attcagtccg ttcattaccg
    16681 cgagctccgg gcgtccattc gacatcaccg tcggccgcga cctcaatggg gattccttgt
    16741 tcaatgaccg gccggcgctc gcgaccgact tgagccgggc gagcgtggtg aggaccgctt
    16801 acggcatctt cgatacgaac ccgctgccgg ggcagatcat cattccgagg aattacggcg
    16861 acagcccggg tcagttcacc atcaatatgc ggctgagcaa gacgtttggg tttggcggcg
    16921 agacggcgag atcggcggac gcgtcccagc agggcatgcc ttccggcccg cgccttggag
    16981 gccctggcgg gcacggcggc gggccgagag gcgggggtgg tttccgcgga ggacccggtg
    17041 gcatctttgg cgaagcatcc accggaaaac gctacaacct gacgttctcg gtttcggcgc
    17101 ggaacctgct gaatcacgtg aatctcgggt ctccgatcgg aaatctgagc tcgcccttgt
    17161 tcggtacgtc caattcgctc gccgggggct tcggtccttt cagctcgacg acagccaacc
    17221 gccgcgtcga acttcaacta aggttcagtt tctagtgccg ggtagacgat tttcttttgt
    17281 cgtctgccgc cctcgcgggt cacggaagac gatggcccgc gccacaattc tcgactacga
    17341 tagcggcatg gagaagcgat tcccgaacgg aaacgtgctc cggctggtaa cgggcgacat
    17401 cacgagcata ccggcggacg cgatcgtgaa cgcggcgaat tcggcgctgg cgggcggcgg
    17461 cggagtggac ggtgcgatcc accgcgccgg cgggccgtcg atcatgcggg aactggatcg
    17521 gatccgagcc agtgcgggcg gctgcccgac gggaagtgcg gtggcaactg ccgcgggggc
    17581 tctgccagcg cgctgggtat tccacgccgt cgggccggtg tatcgcgacg ggaggcgggg
    17641 cgaagcggag ctgctggcat cctgctaccg cacgtgcctg gcgctggcgg aggagcgcca
    17701 ggcggtctcc atcagctttc cgtccatcag caccggcgcg tacggctatc cgattgagga
    17761 agctgcgccc atcgccctgc gcaaaatcag tgcgcacctg gagagcggga cggggagtgt
    17821 gagggaggct ctcgttgtgc tgttctcgaa gtccgactac gagatctacg accgggcgct
    17881 ggcgggactc ggcggcgagc agctagaata gcagttacgt gcaggtctta caggcaggaa
    17941 accaaagata tctgttgctc gaactggaaa ccgaactggt ggaacggatc gccacgcaga
    18001 gcggtttcgc gtgcaagatc gaggactacc cgaggagcat cgttgcggaa ttgtcgatgg
    18061 aggatcgcaa ggcgcctctg ctgctgttcg acgctgccga tccggggaac ctcggctggt
    18121 tttcgcgctg ccagttctat gtggacgggc agaccggggc ggtgctacag actcctattt
    18181 cgctggccaa cctgaaggac cgcggcggac gaagcgtccc gaattccgtg cggctccaga
    18241 tcgcgaagga acttccggaa agcttccgtc ttcccggaaa gcagccggtc acggagcaag
    18301 tcgtctattc cgtgctggcc agtctgctac aggcgctgct cgagagtggc gttggagtat
    18361 gcggcgggac ggtagtacgc ccgctggcgg gacggaccga ggctatcggg ccgcggagct
    18421 gagcgtcggc ccccggcagt ttcgattcca cggcatcttt cgtcgccttc ctggcaccgg
    18481 ctgaccaact cggcgttaca atcgatctga cagagatatc ccgattccgt tttccgaatt
    18541 tggagccgcc catgaccggc cgcaccattt cgcactacca gatcctcgac aagctgggcg
    18601 agggcgggat gggtgtggtt tacagggccc gggacaccca cctcgaccgt ttcgtcgcca
    18661 tcaaggtcct gccgtcggag aaggtcgccg atcccgagcg caagcgccgg tttgtgcagg
    18721 aagcgaaagc cgcctcggcg ctcaaccatc cgaacatcat caccatctac gacattgatc
    18781 aggccgatgg tatcgactac atcgccatgg agtatgccgc cggcaagagg ctggaccggc
    18841 tgatcccccg gcacgggatg ccgctgagcg aagcactcaa gtgcggcgtg cagatcgccg
    18901 atgcgttggc ggccgctcac ggagcgggca tcgtgcaccg cgacttgaag ccgggcaacg
    18961 tcatggtgac ggacaagagt caggtgaagg tgctcgactt cgggttggcg aagctgactg
    19021 agacgggaga aagcggggaa gacttcgaaa cgcgcacgat ccgggacgag gaagggccgc
    19081 gcaccggaga aggcagaatc ctgggcacgg cggcatacat gtcgccggag caggcgcaag
    19141 gcaagaaggt caacgcgagg tcggacatct tctcgttcgg ctctttgctg tatgagatgg
    19201 tgaccggccg gcgggcgttt cagggtgact cggtggtctc gacactggcg gccattatca
    19261 aggagaaccc gacgccgccg agccaggtag tggagggtct tccgaaagag gtagagcggc
    19321 tgatatcccg ctgcctgcga aaagatccgg aacggcgttt ccagcacatg gtggacgtga
    19381 agatggcgct ggaagagctg aaggaggaat ccgattccgg gacgttggag cccgaggggc
    19441 acgcgaaggc gaagcttcgc cgaaagctcc tgtgggcagc cgccgcggtt ggcgtggcgg
    19501 tcctcgcgat ggcgggagta tggtttcttc gctccaggac cccgacgtca gaagcaccgc
    19561 ttgcggctgt cccactcacc agctatccgg gaacagaggg gtcccctagt ttttcccccg
    19621 acggcacgga ggtggccttt cagtggtgtc cggaagaccc cggaaagagt tgcgacatct
    19681 acgtcatgca aatcggcgtg gagcaaccct cccggctaac ggataccccg gcgattgagt
    19741 tcagccccgc ctggtctccg gatggacgga cgatcgcatt catccgccag ctttccgggg
    19801 ctgcgaccag gttcgcattg gttctcatcc ctcagagggg aggacgtgag cgggtgctgg
    19861 gggaagtgga cacgtctgga ccgggcgact ttgtggacgg gccgcacctg gcctggactc
    19921 cggactcgag atggctcgtc tgtccgaggc cagatgccgg aaagggacga tgcagtttgt
    19981 acttgtactc cgtcgagact ggagagaagc gaaagctgac caaccctccc gcgggcgtgg
    20041 taggcgacac agctccggcc ttctcaccgg acggccgtat cctggcgttt gcccgacagt
    20101 tggagggata tttcagatcg gactcttact tgctccatct ggccggggga tacaggccgc
    20161 aaggagagcc ggagagagtt cgattggacg acccgtaccg ctacggtgca gcctggacgc
    20221 cggatggcaa cgagatcgtg tttggaaccg gaaaaggtct ctggcggatg gcggcgtcca
    20281 aaaccgccac gccgcgcagg cttcctttcg ggccggacaa tgccgtcgcg cctgcaattt
    20341 cgaggcaggg gaaccgtctg gcgtattcgg tggaaaggtc ggacaccaac atctggcgga
    20401 ttgacttgcg cgaaccgggc ggcaaaccgg gcgcgccggt ccggttcatc gcatccactc
    20461 tgtcggattg gtgtccggcc tattccccgg acggccagag aatcgccttt gtgtcgaacc
    20521 ggtcggggac ttctgcaatc tgggcatgcg acgccgacgg atccaatgcc atgc
//
LOCUS       DATIFQ010000304        10773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1822886, whole genome shotgun sequence.
ACCESSION   DATIFQ010000304 DATIFQ010000000
VERSION     DATIFQ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822886"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1802)
                     /locus_tag="VLE22_14290"
     CDS             complement(225..1802)
                     /locus_tag="VLE22_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15624.1"
                     /translation="MHEFIARHEEKVIGTLSGFDRLVFRGTLRSISHRQGMEDYLWAN
                     QVLLKEFGPHVERVSRRLKEASLAEAEALGRPVKYLTSSQVSKEEMARGIVAKEGIRD
                     GLVCVLTCVEPCWSFEIHRNRENKKLELEPRYRKCLFLYHYWMHPVLGFMNARIQTWF
                     PFPVQICLNGREWLARQMETAGVEYARQDNCFPWIADWAKAQRLMDRQLRVNWPKLLD
                     GVARQLNPAHREIFKKHPVAYYWSTYQSEWAIDIVFREAAELRRLYPRLVHHGMTTFA
                     SPDVMRYLGKRIPLGGDVPRRFSGDVVSDWKRRQEGVRIKHSVNGNSLKLYDKAFTVL
                     GSVLRAEATIHNGDDFRVYRPKEGDPEGKLAWRQMRRGIADLHRRAEVSRKAAERYLD
                     AFASVDEDTTLEELIRRLGQSRQWRGRRVRALRPFADDSALVAAVSRGEFAITGFRNR
                     DLQAIFFPRAANSPEEARRRSAWVGRKLRLLRAHGLITKVTGTHRYQLTPTGRKAVTA
                     ILTALRSTVRQLTPMAA"
     gene            complement(1930..3078)
                     /locus_tag="VLE22_14295"
     CDS             complement(1930..3078)
                     /locus_tag="VLE22_14295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB15625.1"
                     /translation="MKAQFLASLNHEIRTPLSGILGMTDLLLETELDEEQKDYVGSAR
                     VCAENLLALLNATLEYSDLSAGKLAAEEAEFDLRETLSSAVSQYAQKASAKGLELACS
                     FSPDLPAVAVGDALRLRQLFSHLLDNAVKFTERGEVHFRASSRPAGGGFLLRVEVSDT
                     GIGIQVEKLGAIFESFRQLDSGLSRSYSGLGLGLALSQKLVELMGGALSAESEPGKGS
                     TFSVTLPLRLPDDQGSAHLQSAGAASGGNGARRILVVEDNTVAQRVVTHGLSRGPYKV
                     HCVPSGSEAVRVASAESFDLVLMDLQMPDMDGIETTARLRRLPGYATVPIIALTANTT
                     DEYRRLCRRHGLQGFLPKPVESRKLLETVARFLGCSRGAGHRPAGAVE"
     gene            3528..5213
                     /locus_tag="VLE22_14300"
     CDS             3528..5213
                     /locus_tag="VLE22_14300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15626.1"
                     /translation="MRRSSGIFLLIVFGLWLILPAQAQTPTIRAADGALNGASYARSG
                     LPNGGIAQGSIFVIFGDNLGPATLVQVSAFPLPTTEGLAGTSVTVTVGQTTVTAIMLY
                     TLKTQVAAVLPSNTPLGPGTVRVRYNGQDSNTVPIRVVARSFGTFALNQAGSGPAIIQ
                     NVNSEADRPINAFDHPARPGQVMILWGTGIGPVSGNEPGGALPGNMPGVNTRVFVGSK
                     EASIQYRGRSGCCVGVDQIVFTIPEGVEGCYIPVSVVVDNVVSNYVTIAIARTGNVCS
                     DPGGYSSSQWQTLLSGQNVRIGTLAVQRIFNRNLGGGIGGDGAPYRSDYITAAYSSFS
                     SSALLKVPGQPPIGSCAVMQFPVPSFVIPGALGAGSVTVNGPVGNRVIPMSSTGVYQL
                     LFAPTFPAAPNIVTDGTLVTAGTYAFNVTAGANVGAHSASINHPATFTWNELASFPGT
                     INRNQPFTVTWTGGTAGALVYIYGQSAYDSGPQGDVGASFQCTADGGAGTFTIPASVL
                     SALPPTYSVGPAGPHGSFFMSQYIFGQPLSIPGTDIGTIGVADINTIYTVAYQ"
     gene            complement(5356..8421)
                     /locus_tag="VLE22_14305"
     CDS             complement(5356..8421)
                     /locus_tag="VLE22_14305"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15627.1"
                     /translation="MFKQLLVLGVLCLLIPNLAFAQASAGTGSISGVVTDPTGAVVPQ
                     VEITVRNVGTNVARMVLSNEVGRYEVVALQPGSFEIRASKSGFTSLVRTGVTIAIGAR
                     VAVDLAMSISATVETVTVNADAAAVETDKTDVSTVVNLNDMRNLPLNGRRWDAFVMTT
                     PGATNDGGFGLITFRGISGLYNNNMIDGMDNNQAFFSEAKGRTRLSYGVSSEAIQEFQ
                     VGTSNFSAQYGRSAGGVVNAVTKSGTNDLHGGFFYLIRDDSLNAANSVSGPALRALGL
                     PEKPKDRRQQFGPTLGGSIIKDKLFYFLSYDQQKRTFPAVVVPNSSTFLSATGTAPGF
                     SNVINFYRSLTGPQPRQGDQWLGLSRVDWNLTPRNQISATVNILRWDSPNGIQTAPTH
                     RYHESGNGSDIVKTETLIGRWNAVVTPSFVSEVRVQWGRDFESQVPNAPGPYVAVTNG
                     IDFGMPDFLPRDAYPDERRWQVSQNLNWLTGRHSFKFGYDVTRVADKMINLFQGGGVY
                     NYSVLNDLALDCSNPALPLPLQGCVATSGTGIAGKHYTNFMQQFDTLGQGGKTEFNTV
                     DLAFYIEDSFKPVSTLTVNLGLRYDLQTVPELAGNPDVPATSKINKDTNNFGPRAGIS
                     WDPFGKHKTVIRAGAGMYYGRTQNSTIANFITNNGVRFKGYTFVPANAGSPVFPNVLT
                     EIPTGAAGRPNVLYAAPDFVNPLIYQTEFSIEQEVFKDFTLSAIYLGTRGQKLPLFRD
                     TNLFPSTRTNVYQICGSPQAGSSTACSNVVGAVAVPFFSGARPNTKYGFMTVAESAVN
                     TWYNGFVLQAKQRFSHGIQLQAALTISKAQDNGHKSQTFSTSNQPLNPFDLRQEYGLS
                     DFDQRKRFTMSAYWQPPFARISSKPLRRAFDGFQFSGILTLADGRPYSGMVSGNPSPS
                     GVTSGLLGVGGNARFPGAGRNTYVAPGSAIVDFRVAREIKLSERLSWQLIAEAFNALN
                     RVNITSINMTQYNIRGAVLFPRTDFQSISATGTNLSRERQYQLGTRFTF"
     gene            8577..9797
                     /locus_tag="VLE22_14310"
     CDS             8577..9797
                     /locus_tag="VLE22_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB15628.1"
                     /translation="MSINRREFFASGTAVVSGLTASAQANAAQSIRNEFPRAAGQVYL
                     DAAAHVPLSKYTAEGIRRYMDFHMYGPGEGRGEYVAEALGQVKPLFARLINAKASEIG
                     FVLCTKAGEAAVVNGLDIQASGGNVVTNDLHYAGSLHDYWGRRVAGMDVRIVRHRDWE
                     IDLGDMERVVDRRTRLISITLVSNVNGHVENARALSDLAHAHGAYLYADIIQAAGSVP
                     VDVRTMGIDFAACSAYKWLQGTRGAGFLYVRDELQGSVVKDLVFPGYVEFNYSPWVTS
                     RDTSKEDFPYLARRDASRYEPGNLNYAGYAGQYEALKRILDAGVERMYTHTKPMCDRL
                     KRELPGLGYKLITPVNAASSLVTVQARNLKATTAKLKRANIQVTTAGENRIRISPAIY
                     NNMDDIEKFLAALA"
     gene            complement(10016..10303)
                     /locus_tag="VLE22_14315"
     CDS             complement(10016..10303)
                     /locus_tag="VLE22_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15629.1"
                     /translation="MRRNFWKATLISLGAYTIALLMGFWGDFRFTLFAVPALQVTWTL
                     FCVIRIIQLRIGGGHKPDAPPYDRASYGFALGGSISSFALLAVIVVLTQVV"
     gene            complement(10408..>10773)
                     /locus_tag="VLE22_14320"
     CDS             complement(10408..>10773)
                     /locus_tag="VLE22_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15630.1"
                     /translation="EYANGIKFTFTQVFFHPNGMPGGGQSTWVYGTTGGVDLSTATYF
                     PRQRGAQSVKLMEGAGGPRGSSQYDSHISGFFNAIRTGEPVTAPLKVGCIGTLTAILG
                     REAIYRKKMMTWSDLAVDL"
BASE COUNT         2084 a         3162 c         3494 g         2033 t
ORIGIN      
        1 gggcattgtg cagcgtcacg cgacgccacg cgatgcccac tgcagcgctt ttataagatc
       61 actgtcttgc cgcttcacgg gccgccgtca tcgtgctctg tcgcctgtca acagcgaact
      121 cgccctccga gtgaaacatc cgggctagac tgccgtcatg gcgggtctgg cgacccactc
      181 tctgtactac taacgcctgg aatgttttcc tgcagcgaag attttcatgc ggccatcgga
      241 gtgagttgcc ggacggtaga tcgaagtgca gtgagaatgg cggtcacggc tttgcggcca
      301 gtgggtgtca gttggtaacg gtgcgtgccg gtgactttgg tgatgagacc atgagcgcga
      361 agcagtcgca gcttccggcc tacccaggcc gagcggcggc gggcttcctc cggcgagttg
      421 gcggcacggg gaaagaagat ggcttgcaga tcccggttgc ggaatccggt gatggcgaac
      481 tcgccgcggc tgacggcggc tacgagcgcg ctgtcatcgg caaacggacg aagcgcacgt
      541 acccggcggc cgcgccactg cctggattgt ccgagacgcc ggatcagttc ttccagcgta
      601 gtgtcttcgt cgacgctggc gaaggcgtcc aagtaccgct cggccgcctt gcgggaaacc
      661 tctgcccggc ggtggagatc ggcgatgccg cgccgcatct gccgccaagc cagttttccc
      721 tccgggtccc cttccttggg ccgatagacg cggaagtcgt cgccgttgtg gatggtcgcc
      781 tcggcgcgca gcacactgcc cagcacggtg aacgccttgt cgtaaagctt cagcgagttg
      841 ccgttcacgc tatgcttgat gcgcaccccc tcctgccggc gtttccagtc gctcaccacg
      901 tctcccgaga agcgcctcgg cacgtccccg cccaacggga tgcgtttgcc gaggtagcgc
      961 atcacgtccg gactggcgaa ggtggtcata ccgtggtgga ccagccgcgg gtacagccgc
     1021 cgcaactccg ccgcctcccg aaaaacgatg tcgatcgccc attcgctttg ataggtcgac
     1081 cagtagtagg cgacgggatg ctttttgaag atctcccggt gagcgggatt cagttgccgg
     1141 gcaacgccgt ccagcaactt cggccagttg accctcagtt gccggtccat cagtcgttgt
     1201 gccttggccc agtccgcgat ccagggaaag cagttgtctt gtcgcgcgta ctccactccg
     1261 gccgtctcca tctgccgcgc cagccattcc cgcccgttca aacagatctg cacgggaaag
     1321 gggaaccaag tttgaatgcg tgcattcatg aaaccgagga cggggtgcat ccagtagtgg
     1381 tacaggaaca ggcacttgcg gtagcgcggc tccaactcca gcttcttgtt ctcccggttg
     1441 cggtgaatct cgaagctcca gcacggctcc acgcaggtca gcacacacac caacccgtcg
     1501 cgaatccctt ccttggccac gatgccacgg gccatctctt ccttgctcac ttggctggag
     1561 gtcagatact tcaccggccg ccccaaagct tccgcctccg ccagcgaggc ctccttcagc
     1621 cgccggctca cccgctcgac atgcgggccg aactctttca gtaagacctg gtttgcccag
     1681 agatagtcct ccatcccctg acggtgggag atcgaacgca gggtgcctcg aaagacgagc
     1741 cggtcaaaac cggacagtgt gccgataacc ttctcctcgt gtctcgcgat aaactcgtgc
     1801 atggcgtttc tgtctctcct ccgatgagtg gtcttggaac aaccaaccca tcctacgaga
     1861 tcggaaacgc cgtctttatt tggttgcggc tatgccgcgc tgtgaagccc tgccatcggt
     1921 tcacgtcact cactccacgg ctcccgcagg ccgatggccc gctccgcgcg agcacccgag
     1981 gaaccgggcg acggtttcga ggagcttgcg ggactcgacg ggctttggga ggaagccctg
     2041 aagcccgtgg cggcggcaga gccgcctgta ttcgtcggtc gtgttggcgg tgagggcgat
     2101 aatcggaacg gtcgcgtatc cgggaagccg ccggagccgc gcggtggttt cgatgccgtc
     2161 catgtccggc atctgcaagt ccatgaggac gaggtcgaag ctttcggcgg aggccacccg
     2221 gaccgcttcc gagccgctcg gcacgcaatg aaccttatag gggccgcgac tcaggccgtg
     2281 agtaaccacc cgctgcgcga ccgtattgtc ttccaccacg aggatcctcc gcgcgccgtt
     2341 cccgccggac gccgcgcccg ccgattgcag gtgcgcggac ccctgatcgt caggaagccg
     2401 cagcggcaga gtgaccgaaa aggtgctgcc cttaccgggc tcactttccg cggagagtgc
     2461 gccgcccatc agctcgacaa gtttctgcga cagcgccagg cccagtccga ggccggagta
     2521 ggatcgcgag agcccgctat ccaactggcg gaaggactcg aagatcgcgc cgagtttctc
     2581 cacttggatg ccgatccccg tatcgctcac ttcgacgcgg agcaggaaac cgccgccggc
     2641 gggccgcgac gaggcgcgga aatgcacctc gccccgctcg gtgaatttca ccgcgttgtc
     2701 gagaagatgg gagaacagtt gccgcaggcg cagtgcgtcg ccgacagcga ctgccggaag
     2761 gtccgggctg aagctgcacg caagctccag gcccttcgcg gacgcttttt gagcgtactg
     2821 cgagacggct gaggagaggg tttcgcggag atcgaactcc gcttcctcgg cggcgagctt
     2881 tcccgccgag agatccgagt attcgagggt cgcgttcaga agagccagca ggttctccgc
     2941 gcaaacccgc gccgagccga cgtagtcctt ctgctcctcg tccaactccg tttcaagcag
     3001 caggtccgtc atgccgagga tgccgctgag cggggtacgg atctcgtggt tcaggctcgc
     3061 gagaaactgg gccttcaaac gcctgagctc atccccctgc gccggctcgg gcggagcctg
     3121 accagcggag cgaacacact ctggagccgg agtatccatc gccggtgtct caatcggcgg
     3181 aaagttaacc ggagtttagg gtcagcgggc gtgctgcttc aggaatggcc gaaagcagtc
     3241 aaagttaaga acttttcctc tgcctgaacc ggggaatcaa gagaaggtgg acgggtgtat
     3301 acgaacggta gccagttgca acagcagtta cggcgccgac cgttcttctt cattggtcgc
     3361 acgacataaa cctgacgaag aaaatttgct agaaaggctt gatcaagaga caaacgggtg
     3421 tatactgatt ggcaactgga agccgacgaa gaaattccac gtctggcttg agcctgactt
     3481 actgaacatc cgacgggagt ggtgtcacta cggaggagga cgatccgatg aggcgctcga
     3541 gcggtatctt tctgctaata gtattcggcc tttggctcat actgccggca caggcgcaaa
     3601 cgcctaccat ccgggctgcc gacggtgcac tcaacggagc tagttacgcg cgctccggac
     3661 ttccaaacgg cggaattgcg caaggctcca tcttcgtcat tttcggcgat aacctgggtc
     3721 cggcgacact ggtgcaggtg agtgcgtttc cgctgcccac cacggaaggg ctggcgggaa
     3781 cttcggtcac cgtcactgtg ggccaaacca ctgtgaccgc catcatgctg tacacgctga
     3841 agacgcaggt ggcggccgtg cttccgtcca atactcctct cggcccgggt acagtgcggg
     3901 tgagatacaa cgggcaggac agcaatactg tgcccatccg ggtggtagcg aggtccttcg
     3961 ggaccttcgc gctgaatcag gctggcagcg gtcccgcgat catccaaaac gtgaattccg
     4021 aagcagaccg tcctatcaat gccttcgatc accctgcccg tcccgggcag gtaatgattc
     4081 tatggggtac aggcatcgga cccgtcagcg gcaacgaacc cggaggcgcg ctgcccggga
     4141 atatgcccgg cgtcaacact cgcgtcttcg tggggagcaa ggaagcctcg attcagtacc
     4201 ggggccgctc cggctgctgc gtaggagttg atcagatcgt cttcacgata ccggaaggcg
     4261 tggaaggctg ctacatcccg gtctccgtcg tggtggacaa tgtggtcagc aattacgtca
     4321 cgatcgcgat tgcgcgcacg gggaacgtct gcagcgatcc cggtggctac tcgtcaagcc
     4381 agtggcaaac cctgctctcc ggtcaaaacg tccgcatcgg cactttggct gtacaaagga
     4441 tatttaatag aaacctgggc ggcgggattg gaggagacgg ggccccttac cgcagtgatt
     4501 acattacggc ggcatacagc agcttcagct ccagtgcgtt gttaaaagtt ccaggtcagc
     4561 ctccgatagg tagttgcgcc gttatgcagt ttccagtccc tagttttgtg attcctggag
     4621 cgctgggcgc agggtcggtc acagtgaacg gtccggtcgg aaaccgggta attcctatgt
     4681 catcaaccgg ggtttaccag ttgctttttg ccccgacgtt tccggctgct ccgaatattg
     4741 tgacggacgg gaccttggtc acggccggga cctacgcctt caatgtgaca gccggcgcga
     4801 atgtcggggc tcacagcgcg tccatcaacc accccgccac attcacatgg aacgagctag
     4861 cctcgtttcc cggaaccatt aatcgcaatc agccgtttac cgtcacctgg accggcggaa
     4921 ctgccggcgc gttggtgtac atctacggcc agtcggcata tgactcaggg ccacaaggtg
     4981 acgtcggtgc gtcattccaa tgcaccgccg acggtggtgc ggggaccttt acgatcccgg
     5041 ccagtgtgct atcggcgcta ccacctacct acagcgtggg ccccgcgggg ccccacggct
     5101 cgtttttcat gagccagtat attttcggac agcccctgag tattcctggc actgacatcg
     5161 gcaccatcgg cgtcgctgac atcaacacca tctacacggt cgcctaccaa tagacagccc
     5221 caacgaacac ggcggcacct ctgccgtgag cgagggacaa ggcgggctcc ccgactgccg
     5281 gcagcaagaa accggggaga ggcacccatt tcgcaaaagc ggcgaaatgg agccagtccc
     5341 cgggttttcg gttcatcaga acgtgaagcg cgtgccgagc tggtactggc gctcgcgcga
     5401 gaggttggtg ccggtggccg agatcgactg gaagtcggtc cgtgggaaca gcacggcgcc
     5461 gcgaatgttg tactgggtca tgttgatgct ggtgatattg acgcggttga gcgcgttgaa
     5521 cgcctccgcg atcaattgcc agctcagccg ctcggacagc ttgatttccc gtgccacgcg
     5581 gaaatcgacg atggccgagc cgggggcgac gtacgtgttc cggccggcgc cggggaaccg
     5641 cgcattgccg ccgacgccga gaagaccgga ggtcaccccg ctgggcgagg ggttgccgga
     5701 aaccataccg gagtaaggac ggccgtcggc gagggtcaga atacccgaga actggaagcc
     5761 atcaaacgcg cggcggagcg gtttcgagga gatgcgggcg aacggcggct gccaataggc
     5821 ggacatggtg aagcgcttcc gctggtcgaa atcggagaga ccatactcct gcctcaaatc
     5881 gaagggattc aacggctggt tgctcgtcga gaaggtctgg gacttgtggc cgttgtcctg
     5941 cgccttggag atggtcagcg cggcctgcaa ctgaatgccg tgcgagaacc gctgcttggc
     6001 ctggagcacg aagccgttgt accaggtgtt cactgcgctc tcggcgacgg tcatgaagcc
     6061 atatttcgtg ttcggacgcg cgccggagaa gaacggaacg gcgacggcgc cgaccacgtt
     6121 cgagcaggcg gtgctggagc cggcctgcgg cgagccgcag atctggtaga cgtttgtccg
     6181 tgtcgagggg aagagattcg tgtcgcggaa caaaggcagt ttctggccgc gggtgccgag
     6241 gtagatggca gacagagtga agtccttgaa aacctcctgc tcgatcgaga actcggtctg
     6301 gtagatgagc ggattgacga aatcgggcgc ggcgtacagc acgttgggac gtccggcggc
     6361 gccggtcggg atctccgtca acacgttggg gaagaccggc gaaccggcgt tggcgggaac
     6421 gaaagtgtag cccttgaagc gcacgccgtt gttggtgatg aaattcgcga tggtcgagtt
     6481 ctgtgtgcgc ccgtaataca ttccggcgcc ggcgcggatg acagtcttgt gctttccaaa
     6541 cggatcccag gagatgccgg cccgcgggcc gaagttgttc gtgtccttgt tgatcttgct
     6601 ggtggccggg acatccggat tgccggcaag ctccggcacg gtttgcaggt cgtagcgcaa
     6661 cccgaggttc acagtaaggg tggagaccgg cttgaagctg tcctcgatgt agaaggcgag
     6721 gtccacggtg ttgaactcgg tcttgccgcc ctggccgagg gtgtcaaact gctgcatgaa
     6781 gtttgtgtag tgcttgccgg cgatgccggt gccggaggtg gcgacgcaac cctggagggg
     6841 gagcgggagg gcaggattcg agcagtcgag cgcgaggtcg ttgagcacgg agtagttgta
     6901 gacaccgcct ccctggaaga ggttgatcat cttgtcggcg acgcgcgtaa cgtcatagcc
     6961 gaacttgaag gagtgccgcc cagtgagcca gttgaggttc tgggagacct gccagcggcg
     7021 ctcgtccggg taggcgtcgc gggggaggaa gtcgggcatg ccgaagtcga tgccgttggt
     7081 gaccgcgacg tagggtccgg gggcgttagg gacctgggac tcaaaatcgc ggccccactg
     7141 gacgcgcacc tcactgacga agctgggggt gacgacggcg ttccaccggc cgatgagggt
     7201 ctcggtcttc acgatgtcgg agccgttgcc gctctcgtga tagcgatgcg tgggcgcggt
     7261 ctggatgccg ttgggtgaat cccagcggag gatgttcacg gtggcggaga tctggttgcg
     7321 cggagtcagg ttccagtcga cgcgcgagag accgagccac tgatcgccct ggcggggctg
     7381 gggtccggtg aggctgcgat agaagttgat gacgttgctg aagcccgggg cggttccggt
     7441 ggcgctaaga aatgtggagg agttgggaac gacgactgcc ggaaaagtac gcttctgctg
     7501 atcgtagctg aggaagtaga agagcttatc cttgatgatg gagccgccga gggtggggcc
     7561 gaattgctgg cggcggtcct ttggcttttc gggcaaaccg agcgcccgga gggccggacc
     7621 ggagacggag ttggcggcgt tcagcgagtc gtcgcggatc aggtagaaga aaccgccgtg
     7681 taggtcgttg gttccggact tggtgacggc gttgacgacg ccgccggcgg aacggccgta
     7741 ctgggcggag aagttgctgg tgccgacctg gaattcctgg atggcctcgg aactgacgcc
     7801 gtaagagagg cgggtgcggc ccttggcttc cgagaaaaag gcctggttgt tatccatgcc
     7861 gtcgatcatg ttgttgttgt agaggccgga aatgccgcgg aaggtgatga ggccgaaccc
     7921 gccgtcgttg gtggcgcctg gggtggtcat gacgaaggcg tcccagcggc ggccgttgag
     7981 cggaaggttc cgcatgtcgt tcaagttgac gacggtagag acgtcggttt tgtcggtttc
     8041 gacggcggct gcatcggcgt tgacggtgac ggtttccacg gtcgcggaga tcgacatggc
     8101 cagatccacg gcgacgcggg cgccgatggc gatggtgacg ccggtgcgca cgaggctggt
     8161 gaagccggac ttcgaagctc tgatttcgaa cgatccgggc tggagggcga cgacttcgta
     8221 gcggccgacc tcattggaca ggaccatgcg cgcgacgttg gttccaacgt tgcggacggt
     8281 gatttcgacc tgcgggacga ctgctccagt ggggtcggtg acgacaccgg aaatgctacc
     8341 ggtaccggcc gaggcctgcg caaacgcaag gttgggaatc aggaggcaca atacaccaag
     8401 aacaaggagc tgtttgaaca tagaatgatg gatcttcgag aagatggttt gaggtctctt
     8461 gaaacgttcg tagcagaagt tccgagtata cctcagcggg gcagattctc ggccgcggcg
     8521 attcaagcga gctagaatag tcagccagag cgagtacatc cgcgcggagg gctgagatga
     8581 gcatcaaccg gcgagaattc ttcgcatccg ggaccgcagt cgtctccggc ttgaccgctt
     8641 ccgcccaggc gaacgccgcc caatcgatcc gcaacgaatt tccgcgcgcc gccgggcaag
     8701 tctacctcga cgccgccgcc cacgtgcctc tgtcgaagta cacggccgag ggcattcgcc
     8761 ggtacatgga ttttcacatg tacggtcccg gtgagggacg cggcgaatac gtcgccgagg
     8821 cattgggcca agtaaaaccc ctgttcgcca ggctcatcaa cgcgaaagcg tccgagatcg
     8881 gcttcgtgtt gtgtaccaag gcgggcgaag cggcggtcgt caacggtttg gacatccagg
     8941 catctggcgg caacgtggtc accaacgatc tccattacgc cggctcgttg cacgactact
     9001 ggggacgccg ggtggccggc atggacgttc gcatcgtccg gcaccgcgac tgggagattg
     9061 acctcggcga catggaaagg gtggttgacc gaaggacccg gctgatctcc atcactctgg
     9121 tttccaacgt caacgggcat gtcgagaacg cccgggcctt gagcgatctg gcgcacgccc
     9181 acggcgcgta cctgtacgcc gatatcatcc aggctgccgg cagcgtaccc gtcgatgtcc
     9241 gtaccatggg catcgacttc gccgcctgct ccgcctacaa gtggctacaa gggacgcggg
     9301 gcgcgggctt cctctacgtg cgcgacgagc ttcagggctc cgtcgtcaag gatctcgtct
     9361 tccccggcta cgttgagttc aactactctc cctgggtgac gagtcgcgat acgagcaagg
     9421 aagactttcc ctatctggcg cggcgcgacg ccagccgtta cgaaccggga aatctcaact
     9481 acgcgggcta cgccgggcag tacgaagcgc tgaagcgcat cctcgatgcg ggcgtcgagc
     9541 gaatgtacac ccacacgaaa ccgatgtgcg accgcctgaa gcgagagctg ccgggcctcg
     9601 ggtacaagct gatcacgccg gtcaacgccg cgagttcgct ggtgaccgtc caggctcgga
     9661 atctgaaggc gacgaccgcg aagctgaagc gagccaacat ccaggtgacc accgcgggag
     9721 aaaaccgcat ccgtatctcc ccggccatct acaacaacat ggacgacatc gagaaatttc
     9781 tcgccgccct cgcctgatgg agcaaccgaa atccaaccgg cccgattgac atcgttgatt
     9841 cgctgaaagg atgtggcaag gggcggactg gccgccgtgg ggctggttat ccgacggact
     9901 gacttgaagc gagccgaagg cgccctacaa cttcttttcc gggccagttt cgataagcca
     9961 cctatcgccc gcggcacacc cgagaaatac taccgttgca ggatctttct gtggctcaca
    10021 cgacctgagt gagtacaaca atcactgcca gcagagcgaa tgaagagatt gagccgccaa
    10081 gtgcaaatcc gtagcttgct cgatcgtatg gcggggcatc tggtttgtgc ccgccgccga
    10141 tgcgtaattg aatgattctg ataacgcaga acagcgtcca cgtgacttgg agcgcaggaa
    10201 cagcaaacag cgtgaaacga aagtcacccc aaaaccccat caggagtgca atcgtgtacg
    10261 ctccgagcga aatcaatgtt gccttccaga agttccgtcg catgagctac aacgtccctc
    10321 cacaatctcg tagtctatca ggcccacgtc tgcatagagg caagaaagcg tgacgcggag
    10381 tgggctccgc gtcacgggat tgcgcgctca gaggtcgacg gcaaggtcgg accaggtcat
    10441 cattttcttg cgatagatgg cttcgcgccc gaggatcgcg gtgagtgttc cgatgcagcc
    10501 gaccttcaga ggcgcagtga ctggctcgcc ggtgcggatg gcgttgaaga agccgctgat
    10561 gtggctgtcg tactggctgg agccgcgcgg gccgccggcg ccttccatca gcttgacgga
    10621 ctgcgcgccg cgctggcgcg ggaagtaggt ggcagtgctg aggtcgacgc cgccggtggt
    10681 tccgtaaacc cacgtggatt gaccgccgcc gggcatgccg ttgggatgga agaagacctg
    10741 ggtaaacgtg aacttgatgc cgttggcgta ctc
//
LOCUS       DATIFQ010000305        28232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2590788, whole genome shotgun sequence.
ACCESSION   DATIFQ010000305 DATIFQ010000000
VERSION     DATIFQ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 28232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28232
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590788"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VLE22_14325"
     CDS             complement(<1..741)
                     /locus_tag="VLE22_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15631.1"
                     /translation="MRSLVALALFASSLAAAVDGTVMNRTAGKPQAGALVTLYKLGEA
                     GMQPVSAAKSSAEGKFRFEETIQGPHLLQTIYSGVTYTQMIPPGSPSSDLTVEVFDTS
                     AKPDSTKVSQHMILLEPTENQLVVSENILYQNRGNRTYNDPAGGTVKVFLPETTKGVA
                     HVMVTAPGGMPVQRSLSKTSQANVFKIDFPVKPGETRFDISYSVPFSSPGSFASKVLE
                     KDSPVRLIVPAGVTLAGDGLEPLGQEPST"
     gene            complement(738..1172)
                     /locus_tag="VLE22_14330"
     CDS             complement(738..1172)
                     /locus_tag="VLE22_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSB15632.1"
                     /translation="MIVTAAVLITLGVILFTLGVRRQDLPEPALASPTAHLEERKAAI
                     YENLRDLQFEYRVGKLSDDDYQQSKLVLQKELAGVVAEIDRIAGVPAAPSAPLPKPAS
                     SPTPAAVATAAPASSRCPNCGASFPQPMKFCGECGSPMTEGA"
     gene            complement(1263..3275)
                     /locus_tag="VLE22_14335"
     CDS             complement(1263..3275)
                     /locus_tag="VLE22_14335"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HSB15633.1"
                     /translation="MENLGSLALLLAFCLGVYAFLGSVVGGWKRKPFLVLSAQRAVYG
                     TWFLLTVASALLVQALLTSDFRLGYVAAHSNRDMPTLYKFAAWWGGQEGSLLFWSWLL
                     SSYSAVVVFTNRRKHRGMMPFVTAVLMATQVFFLILNAFVVSPFQVLAVGREVTAVPD
                     GNGLNPLLQYWAMAIHPPFLYLGYVGFVVPFAFAMGSLITRQPGDAWIHTTRRWTIVT
                     WMFQSIGILLGAGWAYAVLGWGGYWGWDPVENASLLPWITATAFLHSVMMQEKKGMMK
                     VWNMVLVSLTFWLCIFGTFLTRSGVVSSVHAFAQSPIGKYFVSFLAIVAAFTIYVILD
                     RLDYLKSESRLESVVSRESSFLFNNLILLASCFAVLWGTLFPVISEAVTGEKISVDAP
                     FFNRVNVPVGLFLLFLTGVAPLLAWRRTSVDGLRRNFLWPAAGAVALMIGLAAAGVRH
                     FYALICFGLCLFVAWTIAAEFIKGARAIRARSGQNLIASMVELTHRNTRRYGGYLVHM
                     GIVLMFVGFAGSAFNKDRTIEVNIGDSFKLASYELTIKDLREGDNPNYSWHHAVVGIS
                     KGGSSLGVLEPERRFYKSSRQGTSEVAIRRRLNEDLYLNFAGMNDSGSKAVIQAYVFP
                     LVSWIWIGFWVLLFGTLVCLVPNKVRLQYARTEVVGVSETYANVEN"
     gene            complement(3289..3714)
                     /locus_tag="VLE22_14340"
     CDS             complement(3289..3714)
                     /locus_tag="VLE22_14340"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HSB15634.1"
                     /translation="MKTYGKFVALIVVIVGTLVWLAVGGINQTKTYYKTIGELREMGN
                     GALGKRLRVAGDVESGSIVRTGREVAFTLHQEDVRLKVVYDGAEPLPDTFRDGAQALA
                     DGRMGTDGVFRASKIQAKCASKYEAKPGQKAPAAKQAGV"
     gene            3785..4522
                     /locus_tag="VLE22_14345"
     CDS             3785..4522
                     /locus_tag="VLE22_14345"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB15635.1"
                     /translation="MGPENVTDLYRRRHSAGWRERLFRILNPPDLCLPDPAELKIDAP
                     LGRWNLYIGGAGCFVDGYVNVDLFPLPGVTVAASAERLPFRSNLFQRVECDAVLEHVE
                     DPEAVVAEIERVLSPGGYAHFVVPFCHPFHEYPRDYRRYTLDGLKQQAGGLEVIASGW
                     RTGPTATLLVFVLEYVKAWLPWRPARVVAHGVLGWLFFPLRYLDVILRRSPRVGRIGN
                     HCYVWARKNGQTATLRSAPSLSAPRGG"
     gene            complement(4476..6578)
                     /locus_tag="VLE22_14350"
     CDS             complement(4476..6578)
                     /locus_tag="VLE22_14350"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HSB15636.1"
                     /translation="MNASRFAAAITVLLAISAYRAAGDEGIWLPNQFPAGEVQKKHGA
                     IVSQDFLDRLRLASVRVVASGSFVSPNGLLFTNHHVASECIQQLSSKEHDYMKDGFYA
                     ANTSGEKRCPGMEADVLLEIEDVTGKVVAGVAEDAAASEASKVRQVNTAQIENECTTR
                     TGNRCEVVTLYSGGQYHLYQYGRYDDVRLVFAPESSVAAFGGDIDNFTYPRFCFDITF
                     LRAYENGKPAATPNYLRWSRRGVRDGELIFVSGHPGTTGRLNTVAQLEFFRDVSYPFV
                     HARLDSLIRRLQAFSAESAENKRVAADNLSSQQNSFKAYTGFLSGLRDAALMSAKRAE
                     EEKLRGAVNRDPGMRERYGTAWEAISAAYGGFRQDYRPYWLLETAATRGSELMRIARD
                     VVRLSEERGKPNETRLKEYRDSALPSLEASLFASAPISASMETAVIADYLEFMRAGLG
                     ADDPTLQAVLGRRSPREAASRYVGASRLADIAERKRLAANSDAVRKSDDGMIRLALLL
                     DGRARALRKKYEDDVEAVLALNVSKVAQSRYRMHGAADYPDATSTLRVSFGLVRGYET
                     SGGSFVPYATDFEGLFRRVTGVEPFVLPEQWIRAKSALDLETPFNFVSTADTHGGNSG
                     SPTVNLDGEVVGILFDGNLESLPNRFLYTEERSRSVHVSAQSITEALRKVYHAQPLLT
                     ELGLSLPGGLKEKAPTAR"
     gene            6789..8147
                     /locus_tag="VLE22_14355"
     CDS             6789..8147
                     /locus_tag="VLE22_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15637.1"
                     /translation="MTQLGEAIARYHKLIESEPHRNLQWAAELRDRMITAHLTVGGKP
                     VCPFLRPHFLTRRRYDALVKAAEALLSAIDRIKQMALANPALLSRMELLPAEKMLASI
                     DPGYPYLTVTSLLDTHLDNGSLRFVNYNADTAPGVAFGTALDELFYDCPPVKQFRRQY
                     KLSRLGGTKHLVQSVLQAYKEFGGKRKPRIGVLEFRQPFQASEPSEYLLLCELFRKAG
                     CAAEIVSPDQLEYKGGVLRQENFVIDLVFRRVKVQEFLLRFDLSHPLVRAYRDRAVCM
                     VNSFRSELAHKKAIFDLLTDETITAGFPAAERRAIRDYIPWTRVVTETKTTFEGKTID
                     LIEFILKNREKLVLKPNDDSGEEPAYQGWLTDDAGWERALRAATRRPYVVQERVEPTR
                     VVFPVMFYGEMQMRELQVDVHPLAFLGRVHGCASWLSEPVSGGFSSIAGLAPTFILES
                     KN"
     gene            complement(8221..9177)
                     /locus_tag="VLE22_14360"
     CDS             complement(8221..9177)
                     /locus_tag="VLE22_14360"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HSB15638.1"
                     /translation="MKTAILSSMAVAFREVDFPPLKGFTAAAPDGAIVGIIGENGAGK
                     CPLLRLASGLEQPAAGEVVIRGAARFLGPDAALDLEGSDVLLLDQTLARHDAIRRAQA
                     VIRMESARRRGATILFVSHEEELLRTLCDEIWWLDGGCLAASGDPSEVLEKYRRHVAH
                     RLRRWGQTVTLPLSPSLRRGDGRAELLSVETLDAAGRPNMVWQSGEAVAVRVVVRFRS
                     AVEDPVVGIMIRTRIGFEVFGTNTELERVRLGPCREGDVLSVLFSFRCELCPQEYTLT
                     AASHDPNGVWHDWMEDAVAFAVADSRYTAGVANLRAHVSVDR"
     gene            9281..10546
                     /locus_tag="VLE22_14365"
     CDS             9281..10546
                     /locus_tag="VLE22_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB15639.1"
                     /translation="MILPAGTVELTSELKVPRKAHDLEIRGADGGTVIRASERFRGRA
                     LFVVESAARIRVRNITVDGNRAALAKPAGLPPYDVPFVQFTETNGILAEDVEGLVVEG
                     VRFREIAGFAILVARSREVAIEDVEVERSGSRNDKGRNNTTGGILLEEGTSGFRVENC
                     SFRSVLGNGVWTHSLYPSPRNVDGLIRGNRFDTIGRDAIQVGHATKIRVEQNTGRRIG
                     FPLEVVDIEGGGTPVGVDTAGNVDRSVYIGNHFEEVNGKCIDLDGFHHGQVTRNICVN
                     RGKAEDYPSGNFGIAMNNTNPDMESEEILVADNEIDGGKFGGIFAIGRNHRIIRNRMR
                     NLNLARCNETAAVFGCLYLPDEPDFLRSGIYLARRAERPAIARGIVIEDNLITGYNMK
                     TRCIGAAPGVSRKGNRISRNDCRDEVPRR"
     gene            10543..11832
                     /locus_tag="VLE22_14370"
     CDS             10543..11832
                     /locus_tag="VLE22_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB15640.1"
                     /translation="MIDPRVCEGMRQDWNRRAREDANYYVAFGRRDQDDAEFFATAAD
                     VVRNLTRELHRLPRGDVRSRRALEIGCGPGRLMLPMSRYFGDIHGVDISDDMIRLAKE
                     KLRDIPHAYASVTSGADLAGFADESFDFVYSYAVFQHIPSRDVVFNYLSEALRVLKPG
                     GVLRCQINGLPEAAARYDTWNGVRISAEDLVALARKRDFQLLAIEGVSTQYMWMTARK
                     TPRARKREPAIAARIRRITNAHSSEPVAPCRGRFASISLWMENLPAECDLPDLRAFIG
                     QEEGSVFYVGPPEADGLQQLNVTLPEKVPTGLRTLRLEWLGHPVCPDGVLRVIRPGPV
                     VPRILSVTDGIDLLSGTRITSGTLKVTVDEIDRPEDLTATVAGRPLVEAEVFCVDPVP
                     PRHEVNLTLPEALAPGIHLLELTLGRRRFAPVALEIV"
     gene            11983..14784
                     /locus_tag="VLE22_14375"
     CDS             11983..14784
                     /locus_tag="VLE22_14375"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HSB15641.1"
                     /translation="MGQLSVDLGTKTLDTEKSGTRQRTGVSFPRYFTARIEAGTTPYD
                     EVEWELRTATIGSDKGAVIFEQRDVEMPADWSQTATNIVASKYFHGKLGTPERERSVA
                     QLVHRVVDTIAEWGRAGRYFKTGEDVENFRSELAHLMLTQKACFNSPVWFNVGVKEGR
                     GYGWYYDETSDSIRKLTPDQTRPQCSACFINSVKDSLESILDLAKTEGMLFKWGSGTG
                     TNLSALREEEAILSGGGRASGPLSFMKGFDAFAGVIKSGGKTRRAAKMVILNADHPDV
                     EAFIWCKAKEEKKAHTLVDAGYDSSLDGEAYGSVFFQNANNSVRATDEFMYAVAEDRE
                     WWTRSVLSGQPVKRYKARELMRQIAEATHQCGDPGMQFDTTVNRWHPCKNSGRINASN
                     PCSEYMFLDDSACNLASLNLMKFLGPDGQFDTEAFRHAVDTVILAQEILVDNASYPTD
                     RIARNSHDYRPLGLGFANLGAMLMSLGIPYDSEQGRDYAGAVTAVMCGQAYLTSARIA
                     GAVGPFAGYRNNEGPFLEVIKMHGESVSRIRRENVPAALYETAGSVWREAYESGRSFG
                     FRNGQTTVIAPTGTIGFMMDCDTTGIEPELALVKYKKLVGGGVIKIVNNTVPQALIRL
                     GYTPEQVERIVSHIDSTGTIEGAPHLKPEHLPVFDCSLRPQNGARFIHHMGHLKMMAA
                     VQPFVSGAISKTINMPEESTVEEIMEAYIEGWRLGLKAVAIYRDGSKRVQPLNTGKGE
                     KKAVPGAPVTEKIVYRPVRRKLPDERQSITHKFSIGGHEGYITAGLYDDGQPGEIFIT
                     MAKEGSTISGLMDGFATAISFALQYGVPLKFLVDKFSHVRFEPSGWTGNQQIPYAKSI
                     LDYIFRWMGAKFLGPEYTITEAGETPKLRPTEPEPQQSLPFGPAVDDAPLCSECGGLM
                     TRNGSCYKCENCGGTSGCS"
     gene            14922..15896
                     /locus_tag="VLE22_14380"
     CDS             14922..15896
                     /locus_tag="VLE22_14380"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HSB15642.1"
                     /translation="MKRAISLFLSAASVLAAGDVLLVGHKWADSAGFYDTETGKQLAV
                     VPAGVKPHEFALTPDRSVAYLTNYGVDRYTETSPGGNTLTILDLRRRQKAGAIDLGTF
                     HRPHGIEMGPSGRLYITCDFPPSLVVVDPNKRKIVESYELGESLPHMLVLTHDEKAAY
                     TANAGAGSVTLVRFGARKPEARIPVGGVPMGLALSRDGTRLYVATRTANTIAVIDTAT
                     NRVVGKIEIAGNPARLALTPDGKHLLASLIEAGDVAVIDLASQRVAHRFHAGANVEGI
                     TVDPSGRFGYASAQGDNTVVKFSLSDWKEVLRIRTESRPDPLAVLPGF"
     gene            complement(15903..16301)
                     /locus_tag="VLE22_14385"
     CDS             complement(15903..16301)
                     /locus_tag="VLE22_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15643.1"
                     /translation="MLSQALAGQTRELGGRWAATDGKRTFRGTWRAFPQPEPGTCGGD
                     WVLKDDSGNVVAGGTWSVRKSPQEWLGAWLAQLPDGRTLSGSWTAQTKLPPSSEFLTL
                     LESAVRETLTGTWTYGERDGSWSVRTDVRR"
     gene            complement(16375..17910)
                     /locus_tag="VLE22_14390"
     CDS             complement(16375..17910)
                     /locus_tag="VLE22_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15644.1"
                     /translation="MNSAVARLASIERYALPLCLAVQAVLLFPQLDLLPVWGDEHYTI
                     QTASQPLSGVLETVRREVNNPPLHPILVHFWLRGPWPGSTPIKARALSVLFALAATLL
                     LDRLWLRPLPGRCRIWYLALWCLSPYMLLYSRMARAYTLQAALFCAALWFATRLFTGQ
                     GKLGLWLGFILTSLALLYVHYLPGMAVLASVAAVFLWKAVRERKPRLLGGLMASAGLI
                     VILYLPWLLYLQAAMGRVARAAAYSPFGESVTSHALRLAYLFFSFHFGETPPEWVVAA
                     SVVLAPLLSFCLWKAAARPPVWFALLAITAVAGYLAASRWVSFAFVPARLVFVYPFFL
                     LLLVLGLERAGRRLRVICLTGLVCLSAASIIQYFQREGFLNKAYLMPYDEAARLIQSG
                     TPGTDTVVIVDMYNNDPWPLVARLPDGVRVVQVAPGRSFESLRSEVATQRARTVWYFR
                     NTHDVSPGGINRKLETELSAGRQVRSHLFLPYSRLDRWMMQLLGWPERPTHVIQMLEI
                     RGP"
     gene            complement(18149..18769)
                     /locus_tag="VLE22_14395"
     CDS             complement(18149..18769)
                     /locus_tag="VLE22_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15645.1"
                     /translation="MKLVMLAAVVAVLAGGAQAPLPERLPSRRDSPEQATLVRILSVR
                     RVYVDRFGGGDTAQQLRDMIISSLLATKLFVITENEARADAFLRGSAEDLIFTDTFSS
                     SESLNARASLSTGTDPDKTSSDRRSRYGGVTVGESESTRTAERKHEAVASVRLVDKDG
                     DVLWSTTQESRGGKFRGASADVAERIAKQLVRDYESARLLRNLPEN"
     gene            complement(18777..19244)
                     /locus_tag="VLE22_14400"
     CDS             complement(18777..19244)
                     /locus_tag="VLE22_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15646.1"
                     /translation="MSAGSLATRLAVLCALFAAISAAVEPGARVAYVGGTVAGVGNRI
                     QGLILMTDDDCFSFQAKAMTVQIPYENINLLEYGQKASRRYAMAIVISPMLLLSKKRN
                     HFLTVGFTDEQGKQQALVFQLDKNDVRAVLAGLEARTGLKVQHQDDEARKGKG"
     gene            19419..20795
                     /locus_tag="VLE22_14405"
     CDS             19419..20795
                     /locus_tag="VLE22_14405"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HSB15647.1"
                     /translation="MQPRDTGAALVNPNMGWVFHHYDNNITKYGLELEPSDTVDEFPG
                     ASTVYLRLAWSYIEPEEGKFNWSIVDTPAQRWIAKGKQVAFRFSCAESDRNQPYATPE
                     WVRKAGAKGYNFVPRKGVVADGPWWEPDYDDPVFLEKLGRFLAAAAARYDGNPEVAFI
                     DIGSIGVWGEGHTYASSMLPFSPDTVRKHIDLHARYFKKTLIAANDDLALHGRGLEVL
                     HYARQHGMTLRDDSILVQPGDKAYHRAYLAPVFWPELPVVLESEHYGASRDEGTWRDG
                     SRYLDAVEDYHASWATVHWYPREFLKEARPLIDKINMRLGYRLQLLEASWPEEVKAGG
                     TMSVGYRWRNAGVAPCYPGGHPAITLKDAKGGIAGVFVDEEFDMRSLPVGAPGQAHPV
                     GREVKAISQASRPLIDFRLPPTNILKPGAYDVFSSVGTRTGTPKIALPLGAEDGQRRY
                     LLGTITVK"
     gene            20792..22258
                     /locus_tag="VLE22_14410"
     CDS             20792..22258
                     /locus_tag="VLE22_14410"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HSB15648.1"
                     /translation="MKIGQVVPFLFSAALACAAAFSQSRVVVKPQDTGAGLTNPGMGW
                     VFHHYDNSIQNYGVDLEPSDTVDEFPGVGVIYLRLAWSHVEPEEGKFNWSVVDTPAQR
                     WIAKGKQVAFRFTASESGKDQPYATPEWVRKAGAKGHFVDPSKGIVPGGFIWEPDFDD
                     PVFLAKLDRFLAAAAARYDGNPEVAFVDVGAFGVWGEGHTYSGSLLPYSAATIRAHID
                     LHRKHFKQTLLAANDDFSLQGRGIETLDYARRLGLTFRDDSIIVQPGDKAWYHAYMAD
                     WFWPRTPVILESAHYGYMLERGNWGDGSLYLKAVEVHHASLLSVHGYPKVFLKDNRAL
                     IDRINRRLGYRLQLLEASWPVEVKAGGTMSVGYRWRNAGVAPCYPGGHPAITLKDAKG
                     GIAGVFVDEEFDMRTLPVGPPDEAQAVGREVEGYSLANQASRPMIEFRLPPDHILKAG
                     AYDLYVSVGSRSGTPKIALPLPGDDGQRRYLLGKVTVR"
     gene            22350..22487
                     /locus_tag="VLE22_14415"
     CDS             22350..22487
                     /locus_tag="VLE22_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15649.1"
                     /translation="MLDLTRRQRSDPEERKVIFLEFVAASAIFSIVALVLYMVFTYKP
                     A"
     gene            complement(22468..23847)
                     /locus_tag="VLE22_14420"
     CDS             complement(22468..23847)
                     /locus_tag="VLE22_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HSB15650.1"
                     /translation="MNPIAYSTIMAVPDTSAGALAERIVGMLQTSGHRAYLVGGCVRD
                     LLLGREPKDYDVATSARPEEILALFPNSRQVGAHFGVMVVTEADTQVEVATFRSDHAY
                     RDGRRPVSVEFEADPRRDVLRRDFTINALLLDPGTGEVLDLVGGRADLEARCVRAIGD
                     PETRFGEDHLRMLRAVRFATTLEFHIEPGTMEAIRRLRGLIRRVSAERVRDELVSILI
                     EGDARRGFELLDESGLLAEILPEVAAMKGVAQPPEFHPEGDVWTHTLLMLGAMRNPTV
                     TLALGVLLHDVGKPPTFRIEERIRFDGHVEVGARMAVEILSRLRFSAAEIRRVEVLVA
                     NHLRFMEVRRMRESTLKRFLRQEHFDEHLELHRLDCAGSHRRLDNYEFVSSKLSELKT
                     EELKPPRLLTGRDLIAAGYHPGPAFHRMLAEVEDAQLESRIRTRDDALALVRSLFDPP
                     DGRPRQACT"
     gene            23861..26080
                     /locus_tag="VLE22_14425"
     CDS             23861..26080
                     /locus_tag="VLE22_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HSB15651.1"
                     /translation="MSSQAGQPAPHAKIVVATTVALSFISFWRGAAIVLSDLASSSFY
                     AGGIAEQAIGKSAPWFVLGVMLFSFAVRSIYMESCSMFVRGGVYVVVRDSMGPVMAKL
                     SVSALVFDYILTGPISSVTAGQYLGHLLNEIAGLVNQPYRVDPHLFAAVFGAAVTIYF
                     WWSNVKGIHESSTKALRIMQITTVMVVLLLIWCPITLLLKDKVDLPPPPTPANLQFTE
                     ESLGWFHDTSWPQIPLVAIMIAFGHSFLAMSGFETLAQVYREIASPKLRNLKRTANIV
                     CGYAVISTGIVTLLAVTIIPDEVRKNYYDNLIGGLAMNLAGPYLLRLGFHVFVVIVGI
                     MILSGAVNTSMIGANGVLNRVAEDGVLVEWFRKPHHRFGTTHRLINLVTLFQLITIIA
                     SRGDVYLLGEAYAFGVVWSFSLKGLGVLVLRFQRKDQEYKTPLNFHIGGREWPVGLAM
                     TTLVLFFVAVANLFSKRIATIYGVSFTVLLFVVFTVSEYLNRRKRSAVHKLGLEEFNL
                     EIQPQVTAETIEARPGCVLVAVRDYSRMVHLQKVLEKTNLRRHDIVVMTVRPVTAGAG
                     EYGLAANQIFSSYEKELFTAVVSMAEKEGKPVDLLVVPAVDPFDAMVQTASELQASRL
                     VTGVSARMDSEELARLIGLAWERLPEPRHAFSLEVISPDRPSIYVNLGPHPPRLWPED
                     IDRLHDIWLQLSERAGFGSKLHHRDVVGLALRRLQKQLDAKDREQVIEDLRRELGKG"
     gene            complement(26267..26542)
                     /locus_tag="VLE22_14430"
     CDS             complement(26267..26542)
                     /locus_tag="VLE22_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15652.1"
                     /translation="MSDLRLGDVIDDYCVKCRRLTNHAIVSLLNAEPAKVRCRTCYND
                     HDYRHEKAPPSKKELKKAALFKEVLSSVDPTEGETDKRQKDKPEGKS"
     gene            complement(26586..27104)
                     /locus_tag="VLE22_14435"
     CDS             complement(26586..27104)
                     /locus_tag="VLE22_14435"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HSB15653.1"
                     /translation="MNVVILYLILLKATMTSFSGLASLPMLRNDLVVQHRVLTDRELN
                     TAVAVGSMGPGPLGLYVVSVGYFVAGVPGAFAGMLALMTPAFLIVPMVRHFGRRADHP
                     RAKQVIQSVTLAAAGLILSASVPLARDAITGVVPALLAGAVFVLMVATRIDTVWMIVG
                     SAAAGLLWQVVQ"
     gene            complement(27101..27646)
                     /locus_tag="VLE22_14440"
     CDS             complement(27101..27646)
                     /locus_tag="VLE22_14440"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HSB15654.1"
                     /translation="MPSLRTLFSIALRIGNLTFGGGEPTMAALQRELVSRKGWISADQ
                     YGLSYGLARITPGTNLLAFCAAAGWFIRRWPGAFAAVAAVTVPSAVLAVILTHSYQQL
                     RAHPAAVGAFNGIVAAAVGMMAAAAWLLIRSQAAGSSRLRPWALVAAALILSLGFSLS
                     PIPVLALAAITGLLWPEPAKR"
     gene            complement(27665..>28232)
                     /locus_tag="VLE22_14445"
     CDS             complement(27665..>28232)
                     /locus_tag="VLE22_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HSB15655.1"
                     /translation="YIMALHDRAFGHKGLLFFADCSIVIDPSAVQLADIAIATAGSVR
                     NLLGVEPAVALLSFSTKGSATHKAVDKVAEALRIIRARDPNLHADGELQADAALVEAV
                     GRSKAPGSTVAGRANTLIFPNLEAANIAYKLVERLAGATAIGPLLQGLAKAGNDLSRG
                     CSAEDIYNVAVITANQAEAMRQASSDPY"
BASE COUNT         5498 a         8562 c         8863 g         5309 t
ORIGIN      
        1 tgtgctcggt tcctgtccga gaggctccag tccgtcgccc gcgagcgtca ctcccgccgg
       61 gacgatcaat cgaacgggag aatccttttc gagcaccttc gaggcgaacg aaccaggact
      121 gctgaacgga accgaatagg agatgtcgaa gcgggtctcg ccgggcttga cgggaaagtc
      181 aatcttgaag acgttggcct gactggtttt cgagagcgat cgctgtaccg gcattccgcc
      241 cggggccgtt accatcacgt gcgccacgcc ctttgtggtc tcgggcagga acactttcac
      301 ggtgccaccg gcggggtcgt tatacgttct gttgcccctg ttctggtaga ggatgttctc
      361 gctcacgacc aactggttct cggtcggctc cagcagaatc atgtgctgcg agactttcgt
      421 ggagtccggc tttgcggacg tatcgaaaac ctcgaccgtg aggtcgctcg agggcgaccc
      481 gggagggatc atctgcgtgt aggtgacgcc gctgtaaatg gtttgcagca ggtgcggtcc
      541 ctggatcgtc tcctcgaaac ggaacttgcc ttcagcgctc gattttgctg cggaaaccgg
      601 ctgcatgccc gcctcgccaa gcttgtagag ggtcaccaaa gcgcccgctt gcggcttgcc
      661 ggctgtacgg ttcattaccg tgccgtcgac ggcagccgcc agggatgatg cgaacagggc
      721 cagagccacc aggcttctca tgctccctcc gtcatcgggc tgccgcactc gccacagaat
      781 ttcatcggtt gcgggaagct agcgccacaa tttggacacc gactcgaagc aggcgcggct
      841 gtcgcgacgg cggccggcgt cggtgaggag gccggtttcg gcaagggtgc actcggggcg
      901 gccggcacgc cggcgatccg gtcgatttcc gctacaacgc ccgccagttc tttctggagg
      961 acgagtttgc tctgctggta atcatcatcg gagagcttgc cgacacggta ttcgaactga
     1021 agatcgcgga ggttttcgta gatggcggcc ttgcgctcct cgaggtgggc agtcggggag
     1081 gccagcgccg gttccgggag gtcctgcctg cgcacgccaa gcgtaaacag gatcaccccg
     1141 agggtgatga gaacagcggc ggttactatc atcaatccag tttgtccagg tcttattcga
     1201 tcgtcgtccg gcggcgggca gccgcctcct caggcggtgt caaagggagt cgaagcgggc
     1261 tgctagttct ccacgttggc atacgtttcg gaaacgccca ccacctccgt gcgtgcgtac
     1321 tgcaatcgaa ccttgttcgg cacgaggcag accagggtgc cgaacagcag cacccagaac
     1381 ccgatccaga tccatgacac tagcgggaac acgtaggcct ggatcacggc cttcgacccg
     1441 ctgtcgttca tgccggcaaa gttcaggtaa agatcttcgt tcagccgccg ccggatggcg
     1501 acttcggatg tcccctggcg gctcgattta tagaagcggc gttccggttc caatacgccg
     1561 aggctgcttc cgcccttcga aatgccgacc acggcgtggt gccaactgta gttcgggttg
     1621 tctccttcgc gcagatcctt gatcgtgagc tcgtagctgg cgagcttgaa cgagtcgccg
     1681 atgttgacct cgatcgtccg atccttgttg aacgccgaac ccgcaaagcc gacgaacatc
     1741 agcacgattc ccatgtgaac gaggtagccg ccatagcgcc tggtattgcg gtgggtgagt
     1801 tcgaccatgg aagcgatcag gttctgaccg cttctcgcgc ggatggctcg cgcgcccttg
     1861 ataaactcgg ccgcgatcgt ccacgcgacg aacaggcaca gtccgaagca gatcagcgca
     1921 tagaagtggc gtaccccagc cgcagccaga ccgatcatga gcgctaccgc accggcggcc
     1981 ggccagagga agttcctccg cagaccgtcc acggacgtcc ggcgccaggc cagcagcggc
     2041 gccacgccgg tcagaaacag caggaacagc ccgactggca cgttcacgcg gttgaagaac
     2101 ggggcgtcca cactgatctt ctcgccggta accgcctcgg agatcaccgg gaacaacgtg
     2161 ccccaaagga cagcgaagca gctcgccagc aggatcaggt tgttgaacag gaagctggat
     2221 tcacgcgaga cgacgctttc aagccgcgac tcgctcttca ggtaatcgag ccggtccagg
     2281 atcacgtaga ttgtgaaggc ggcgacgatg gccaggaaac tgacgaagta cttgccgatt
     2341 ggagactgcg cgaaggcgtg taccgaactc acgacgccgg aacgcgtgag gaacgtcccg
     2401 aagatgcaga gccagaaagt cagggaaacg aggaccatgt tccagacctt catcattccc
     2461 ttcttctcct gcatcatcac cgagtgcagg aacgccgtcg cggtgatcca cggcaggagc
     2521 gaggcattct ccacggggtc ccagccccag tagcctcccc agccgagcac cgcgtaggcc
     2581 cagcctgctc cgagcaggat gccgatgctc tggaacatcc aggtcacgat cgtccaacgg
     2641 cgcgtcgtat ggatccacgc gtcgccaggc tggcgcgtga tcagcgaccc cattgcgaag
     2701 gcgaatggca ccacgaagcc gacgtatccg agatacaaga agggtggatg aatcgccatc
     2761 gcccagtact gaagcagcgg gttcaatcca ttaccgtccg ggaccgcggt gacttcccgg
     2821 ccaaccgcca gcacctgaaa ggggctgacc acgaaagcgt tgagaatcag gaagaagacc
     2881 tgggtcgcca tcagcacggc ggtgacgaaa ggcatcattc cgcgatgctt tcgccggttg
     2941 gtgaagacca cgaccgccga gtaactggaa agcagccagc tccagaacag cagcgagcct
     3001 tcctggccgc cccaccaggc cgcaaacttg tacagggtcg gcatgtcacg gttgctgtgc
     3061 gccgcgacgt agccgagccg gaagtcgctg gtcagcaaag cctggacgag cagggcggag
     3121 gcaactgtca gcagaaacca cgttccgtat accgcgcgtt gggcgctcag gacgagaaac
     3181 ggcttgcgtt tccagccacc gacgaccgag ccgagaaacg cgtatactcc caaacagaac
     3241 gcaagaagga gtgcgagcga acccagattt tccataggat ccttccggct atacgccggc
     3301 ttgtttcgcg gcgggggcct tctggccggg ctttgcctcg tatttcgagg cgcacttggc
     3361 ctggatcttg cttgcccgga acacgccgtc cgttcccatc ctgccgtcgg ccagggcttg
     3421 cgcgccgtcg cggaaggtgt ccggcaaagg ctcggcgccg tcatagacga ccttcagccg
     3481 cacatcctcc tgatggaggg tgaacgccac ctcgcgcccg gtgcggacaa tcgagccgct
     3541 ttcgacatcg ccggcgacgc gaagacgttt acccaacgcg ccgttgccca tttcgcggag
     3601 ctctccgatg gtcttgtaat acgtcttcgt ctggttgatt ccacccaccg ccagccagac
     3661 gagagtgcct actataacga caatcagagc cacaaacttg ccatacgttt tcatgatgga
     3721 agccctttgg tctcagtata ccagtctggg gcggagtgtt accttggtag ttctccctcc
     3781 ccatatgggt ccggaaaatg taacagatct gtaccgccgc cgtcattccg cgggctggag
     3841 ggagcgactt tttcgcattc tcaacccccc ggacctctgc ctccccgacc cggccgaact
     3901 gaagatcgac gcgccgctcg ggcgctggaa tctctatatc ggcggcgcag gctgctttgt
     3961 cgacggctac gtgaacgtgg acctcttccc gctcccgggc gtcaccgtcg ctgcctccgc
     4021 ggagcggctt cccttcagga gcaacctctt ccagagagta gagtgcgacg cggtcctgga
     4081 gcatgtggag gacccggagg ctgtagtcgc cgaaatcgag cgcgtgctgt cccccggagg
     4141 ttatgcccac tttgtagtgc ccttctgcca cccgttccac gaatatccac gcgactaccg
     4201 ccgctacacg ctggacgggc tgaagcaaca ggccggaggc ctggaagtga tcgcctcagg
     4261 ctggcggacc ggtcccacgg caacgctact ggtcttcgtt ctcgagtacg tcaaggcctg
     4321 gcttccgtgg cggccggctc gcgttgtggc gcacggcgtc ctcggctggt tattctttcc
     4381 gctgcggtac ctggacgtga tcctccgccg gtcgcctcgg gtcggccgta tcggcaacca
     4441 ctgctacgtc tgggcccgga aaaacggtca aaccgctacc ttgcggtcgg cgccttctct
     4501 ttcagccccc cggggaggct gagaccgagc tcggtaagca acggttgcgc gtggtagacc
     4561 ttccggagcg cctcggtgat cgattgggcc gagacatgaa ccgagcgtga cctctcctcg
     4621 gtgtacagga aacgatttgg aaggctttcc aagtttccgt cgaacaggat tccgacgact
     4681 tcaccatcca ggttcaccgt cgggctgccc gagttgccgc cgtgcgtgtc ggcggtagag
     4741 acgaagttga acggcgtctc caggtcgagt gcggacttgg cgcggatcca ttgctccggc
     4801 aggacgaacg gctccacacc ggtgacccgc ctgaagaggc cctcgaagtc cgtggcatag
     4861 ggaacaaaag aaccgccgga ggtctcatag ccccggacaa gcccaaagga gacgcgcaat
     4921 gtcgaagttg catcggggta gtcggccgcg ccgtgcattc ggtagcgtga ctgagccacc
     4981 ttggacacgt tgagcgccag caccgcctcg acgtcgtcct cgtacttttt ccggagcgcg
     5041 cgggcgcgtc cgtcaagcag caaagccaga cggatcattc cgtcgtccga cttccgcacg
     5101 gcgtcggagt tcgcagcgag cctcttgcgt tcggcgatat ccgcgaggcg gctggcgccc
     5161 acgtaccggc ttgcggcctc gcgggggctg cgtcttccga gcacggcctg caaggtcgga
     5221 tcgtccgcgc cgagacctgc ccgcatgaat tcgaggtagt cggcgatgac ggcggtctcc
     5281 atcgacgcgc tgatcggagc gctcgcaaac agagaggctt cgaggctcgg aagagccgag
     5341 tcgcggtatt ccttgagccg tgtttcattc ggctttccgc gctcttcgga gagccgcaca
     5401 acatcccggg cgatacgcat cagctcggaa ccccgcgtgg ccgccgtctc gagtagccag
     5461 taggggcgat aatcctgccg gaagccaccg taggcagccg agatcgcttc ccatgccgtg
     5521 ccgtagcgct cccgcatgcc gggatcacga tttacggcgc cgcgcaactt ctcttcctcc
     5581 gcgcgcttcg cgctcatcaa cgctgcatcg cgcaggccgg acaagaaacc cgtgtatgcc
     5641 ttgaagctgt tctgttggct cgacaggttg tcggcggcga cgcgcttgtt ctcggcgctc
     5701 tcggccgaga atgcctgcaa cctccggatc agcgaatcca gacgggcatg aacgaacgga
     5761 taggagacgt cacggaagaa ttcgagttgc gccacggtgt tcagccgccc ggtcgtgcct
     5821 gggtgcccgg acacaaatat gagttcgccg tcccggacgc ccctccggct ccaccgcaga
     5881 tagttcggag ttgccgccgg ttttccgttc tcgtaggcgc gcagaaacgt aatgtcgaag
     5941 caaaagcgcg gatacgtgaa gttgtcaatg tcccctccga aggccgcgac cgaggattcc
     6001 ggagcgaata ccagcctcac gtcgtcgtac ctcccgtact gatacaggtg atactggccg
     6061 ccggaataga gcgtcacaac ctcacagcgg ttgcctgtgc gggtagtaca ttcattctcg
     6121 atctgcgcgg tgttgacttg ccgcaccttc gaagcctcgg aggccgcggc gtcctctgcg
     6181 actcccgcga cgaccttgcc ggtcacgtcc tcgatctcga gtaatacgtc cgcctccata
     6241 ccagggcaac gcttctcacc ggatgtattg gcggcataaa agccgtcctt catgtagtcg
     6301 tgctctttcg agctgagttg ctggatgcac tcggaggcca cgtgatggtt cgtgaagagt
     6361 aacccattgg gtgagacaaa agagcccgag gcaacgacgc gcaccgacgc aagccgcagg
     6421 cggtcaagga aatcctggct aacgatggcg ccgtgcttct tctgcacctc gccggccgga
     6481 aactggtttg ggagccagat gccttcatcg ccagccgccc ggtacgcgga aattgccagg
     6541 agcacggtga tcgcggcggc gaacctggat gcgttcatac tgtataagac ccctttctcc
     6601 tctattctcg ccgatttcta atattctctt tgctcggctg attcgccagt ttcgacagaa
     6661 atcggaaaaa atcccataat gcattgatta taaatagctt aactagattt ccctgatggc
     6721 aaaaacttcc cttcttgcca tcggtgtgcc atattaagaa gtgggaagtt aggttataca
     6781 agttgcttat gacgcagtta ggcgaggcaa tcgcccgcta tcacaagctg atcgaatcag
     6841 agccgcaccg gaatctccaa tgggcggccg agctccgcga ccggatgatc acggctcacc
     6901 tcaccgtggg cggcaagcca gtctgcccgt ttctccgtcc gcactttttg acacggcgcc
     6961 gttacgacgc gctggtgaag gcggccgagg cccttctttc cgcaatcgac cggatcaaac
     7021 agatggcttt ggccaatccg gcgctcctca gccgaatgga gttgctgccc gctgagaaaa
     7081 tgctggcgtc gattgatcca gggtatccgt atctgaccgt aacgtcgttg ctggacacgc
     7141 atcttgacaa cgggtcactg cggtttgtga attacaacgc ggatacggcg ccgggtgtgg
     7201 cgtttggaac cgcgctggac gaattgttct atgactgccc gccggtgaag cagttccgcc
     7261 gccagtacaa gctctcgcgg ctcggcggca cgaagcacct cgtgcagtcg gtgttgcagg
     7321 cctacaagga atttggtggc aagcgaaagc cccgcatcgg cgtgctggaa ttccgccaac
     7381 ccttccaagc gtctgagccg agcgagtatc tcctgttgtg cgagttgttc cggaaggcgg
     7441 gttgcgcggc agagatcgtt tcgcccgacc aactggaata caagggcggc gtcctgcgtc
     7501 aggaaaactt tgtaatcgac ctggtgttcc ggcgggtcaa ggtgcaggag tttcttcttc
     7561 gtttcgatct gtctcaccca ctggtgcggg cctaccgcga ccgggcagtc tgtatggtca
     7621 acagctttcg gtcggaactg gcgcacaaga aagcgatttt tgacctgctc acggacgaaa
     7681 cgatcactgc cggttttcct gccgccgaac gccgggccat tcgcgactat attccgtgga
     7741 ctcgagtcgt gaccgagacc aagacgacct tcgagggcaa gacgatcgac ctgatcgagt
     7801 ttatcctcaa gaaccgcgag aagctcgtat tgaagccgaa cgacgacagc ggcgaggaac
     7861 ctgcttacca aggatggctc accgacgacg ctggctggga gagagcgctg cgggcggcga
     7921 cgcgcaggcc gtacgtcgtg caggagcgtg tcgagccaac tcgcgtcgtg ttcccagtca
     7981 tgttttacgg ggagatgcag atgcgcgagc ttcaggtgga cgttcacccg ctcgcattcc
     8041 ttggacgcgt gcacggctgt gcgagctggc tctcggagcc cgtgtctggc gggttctcca
     8101 gcatcgccgg tctggcgccc acattcattc tcgagagcaa gaattagctg ttagcggtca
     8161 gcgatcagct ctcagctttt ggaggagccc cctcagctga tcgccgaccg ctgaaggctg
     8221 tcaccgatcg acggacacgt gtgcccgcag gttcgccacg ccggccgtgt agcggctgtc
     8281 ggcaacggca aacgcgaccg cgtcttccat ccagtcatgc cagacgccgt tcggatcgtg
     8341 ggaggccgct gtaagcgtgt actcttgagg gcacaactcg cagcggaacg agaagagcac
     8401 gctcaggacg tccccttccc ggcagggtcc aagccggacg cgctccagtt cggtgttggt
     8461 gccgaaaacc tcgaagccga tacgagtgcg gatcatgatc ccaacaaccg ggtcctcgac
     8521 ggcggagcgg aagcggacca cgacgcgcac tgccacagct tcaccgctct gccagaccat
     8581 gttgggacgg ccggcggcgt cgagtgtctc gactgaaagc aactccgcac ggccgtcgcc
     8641 gcgccgcaga gacgggctca gtggcaaggt cacggtctgg ccccagcgcc tcaggcgatg
     8701 cgcgacgtga cggcggtact tctccagaac ctcgctgggg tctccggatg cggcgagaca
     8761 gccgccgtcg agccaccaga tttcgtcgca aagcgttcga agcagttcct cctcgtgcga
     8821 tacaaacagg atcgtcgccc cgcgccgccg cgccgactcc attctgatga cagcttgtgc
     8881 ccggcggatg gcgtcgtggc gcgcgagcgt ctgatccagc agcagaacat ccgagccttc
     8941 gagatccagg gcggcgtctg gcccaagaaa tctcgcagcg ccccggatga caacctcacc
     9001 cgcagccggc tgttcgagac cggaagcgag gcgcagaagg gggcacttac ccgctccgtt
     9061 ctcgccgatg attccgacga tagcgccatc cggcgcggcg gcggtgaaac ctttgagcgg
     9121 tggaaaatcg acctcgcgaa acgcgacagc catggaagat agaatagcgg ttttcatgcg
     9181 gaaaccggtt ctagcggtcc ttgtgttgtc ctttgcgggc tgtggtgggc ctagcggtga
     9241 agcggagttg agacgcgccc ttgagcgaac aggaggggtt gtgatcttgc ccgccggcac
     9301 ggtcgagctc acctccgaat tgaaggtccc gcgcaaggcg cacgacctgg agatccgcgg
     9361 agcggacggg ggtaccgtga tccgagcctc ggaacgcttt cgagggcgag ctctgttcgt
     9421 ggtggagtcg gccgcgcgaa tccgggtccg gaacatcacg gttgacggga accgtgcggc
     9481 gctcgcgaaa ccagccggcc tgccgcccta cgacgtcccc ttcgtgcagt tcaccgagac
     9541 gaatggaatc ctggccgagg acgtcgaggg tctcgttgtg gaaggggtgc ggttccggga
     9601 gatcgctggt tttgcgatcc tcgttgcacg ctcccgcgag gtagcgattg aagatgtcga
     9661 agtggagcgc agcggctcgc ggaacgacaa ggggcggaac aatacgaccg gcggcattct
     9721 tctggaagag ggcacctcgg ggtttcgtgt tgagaactgc tcctttcgga gcgtgctggg
     9781 taacggtgtg tggacacatt cgttgtatcc gtcgccgagg aacgtggatg ggctgattcg
     9841 cggcaaccgc ttcgacacga tcgggcgtga tgcgattcaa gtcgggcatg cgacgaagat
     9901 ccgggtggaa caaaacaccg ggcggcggat cgggtttcca ctcgaggtgg tcgatatcga
     9961 gggcggggga acgccagtcg gcgtggacac ggccggcaac gtcgaccgca gcgtctacat
    10021 cggcaaccac ttcgaagagg taaacggtaa atgcatcgat ctcgacggat tccatcacgg
    10081 acaggtgacg cggaatattt gtgtcaaccg cggcaaggcc gaggactatc cgtccggcaa
    10141 ttttggcatt gcgatgaaca acacgaatcc cgacatggag tcggaggaga tcctggtcgc
    10201 cgataacgag atcgatggtg gaaaattcgg agggatcttc gcgatcggcc ggaatcaccg
    10261 cattattcgc aaccggatgc ggaacctcaa tctcgcccgc tgcaacgaaa cggcggcggt
    10321 ttttggatgc ctgtaccttc cggacgagcc cgatttcctg cgctccggaa tctatctggc
    10381 gcgacgagcc gaacgtccgg cgattgctcg cggcatcgtc atcgaggaca atctgattac
    10441 cggctacaac atgaagaccc gctgtattgg cgccgcgccc ggcgtgtccc ggaaggggaa
    10501 ccgcatcagc cgaaacgatt gccgcgacga ggtgccccgg cgatgatcga cccgcgcgtg
    10561 tgcgaaggga tgcgccagga ctggaaccgg cgcgcccgtg aggacgccaa ctactacgtc
    10621 gcatttggcc gccgcgatca ggacgacgcc gagttcttcg ccaccgccgc cgacgtggtg
    10681 cgaaatctga cccgggaact gcaccgcctt ccaaggggtg atgtacggag ccggcgcgca
    10741 ctcgagatcg gctgtggtcc cggccgccta atgctgccga tgagcaggta tttcggtgac
    10801 attcatggcg tcgacatctc ggacgatatg atccggctgg cgaaagagaa gctccgtgac
    10861 attccgcacg cgtacgcgag cgtcacaagc ggcgccgacc tcgccggctt cgccgatgag
    10921 tcgtttgact tcgtgtactc ctatgctgtg ttccagcaca tcccgagccg cgacgtggtt
    10981 ttcaactacc tgagcgaagc gctccgggtg ttaaaaccgg gcggcgtgtt gcgatgccag
    11041 atcaatggcc tgccggaggc ggcggcccgg tatgacacgt ggaacggtgt gcgcatcagc
    11101 gcggaggatc tcgtcgcact cgctcgcaag cgggatttcc aactactggc aatcgagggc
    11161 gtctcgacgc aatacatgtg gatgacggca cggaaaacgc cgcgcgcgcg aaaacgcgaa
    11221 cccgccattg cggcgcgcat ccggcgaatc acgaacgctc acagctcgga accggtggcg
    11281 ccttgccggg gccgcttcgc ctcgatctcg ctgtggatgg agaatctgcc ggcggagtgc
    11341 gacctgcccg atctccgcgc cttcatcgga caggaagaag ggagcgtgtt ctatgtaggg
    11401 cctccggagg ctgacgggtt gcagcagttg aacgtgacgc tcccggagaa ggtgccgacg
    11461 ggattgcgga ctctcagact cgagtggctg ggtcacccgg tttgtcccga tggtgtgctc
    11521 cgggtcatac ggccgggtcc tgtggtgccg cggatactct ccgttaccga cggcatcgac
    11581 cttctttccg ggacgcggat cacgtccgga accctcaaag tgacggtcga tgagattgac
    11641 cgtccggagg atctcaccgc caccgtcgcg ggccgaccgc tcgtggaggc ggaggtcttt
    11701 tgcgttgacc cggtgccgcc aaggcacgaa gtgaacctga cgcttcccga ggcgcttgcg
    11761 cccggcatcc atctactcga attgactctc ggacgccgtc gtttcgctcc ggtagcgctc
    11821 gaaatcgtct gagtgccgcc gcctttccgc tttcatccgc ctgtaacctt ctgcaccgct
    11881 aatagataaa aatcttgact gggtactata tgttggggta caagtgacat tgccgccttt
    11941 gggggcgggc agttgggaat cataatgaat ggaggagaga aaatgggcca gctaagtgtt
    12001 gatctcggca ctaaaactct ggacacggag aagagcggaa cccggcaacg gaccggagtg
    12061 agtttccccc ggtacttcac agccaggatc gaggctggga cgacgccgta cgacgaggtg
    12121 gaatgggagc ttcgtacggc caccatcggt agtgacaagg gcgcggtcat cttcgagcag
    12181 cgggatgtcg agatgccggc cgattggtcg caaacggcca cgaatatcgt cgctagcaag
    12241 tactttcacg ggaagctcgg aactcccgag cgggaacgga gcgtggctca gcttgttcat
    12301 cgcgtggtgg acaccattgc tgagtggggg cgcgccggcc ggtatttcaa gacgggcgaa
    12361 gatgtggaga acttccggag cgagcttgcc cacctgatgc tgacgcagaa ggcctgcttc
    12421 aactcccctg tctggttcaa cgtcggcgtg aaggagggcc gcggctatgg atggtattac
    12481 gatgagacct cggattcgat tcgaaagctg accccggacc agacccgccc ccagtgctcc
    12541 gcctgcttca tcaattcggt caaggattcg ctcgagtcga ttctggacct ggcgaagacg
    12601 gaagggatgc tatttaagtg gggctccggt acgggcacaa atctttccgc cctgcgtgag
    12661 gaagaagcca tcctctcggg cggcggacgc gcctctgggc cgctgagttt catgaaggga
    12721 ttcgacgcat ttgcgggtgt catcaagtcc ggcggtaaga cgcggcgggc ggcaaagatg
    12781 gtcatcctga atgccgacca cccggacgtc gaggcgttca tctggtgtaa ggcgaaggag
    12841 gagaagaagg cccacacgct ggttgacgcg ggctacgatt cgtcgttgga cggcgaggca
    12901 tacggctcgg ttttcttcca gaacgcgaac aactcggtcc gggcgacgga cgagttcatg
    12961 tacgcggtcg cggaagaccg cgaatggtgg acacggtcag tgctttcggg gcagccggtg
    13021 aaacgttaca aggcccgaga gttgatgcgg cagatcgccg aggctaccca ccagtgcggc
    13081 gaccccggga tgcagtttga caccacagtg aaccgctggc atccctgcaa gaacagcggc
    13141 aggatcaatg cgtcgaaccc gtgctcggag tacatgtttc tggacgattc tgcctgcaac
    13201 ctggcctcgt tgaacctgat gaaattcctc ggcccggacg ggcagtttga taccgaggca
    13261 ttccgtcacg cggttgacac ggtgatcctg gctcaggaga tcctggtgga caacgccagc
    13321 tacccaaccg acaggatcgc gaggaactcg catgactacc gtccgctcgg gcttggtttc
    13381 gcgaatctcg gtgcgatgct gatgtcgctg ggcattccgt acgacagcga acaggggcgt
    13441 gattacgctg gcgctgtcac cgctgtaatg tgcgggcagg cctacctgac gagtgctcgt
    13501 atcgccgggg cggtggggcc gttcgccggg taccggaaca acgaggggcc gtttctggaa
    13561 gtcatcaaga tgcacggcga atcggtgagc cggattcggc gcgagaacgt gcctgcggcc
    13621 ctctatgaga ccgccgggtc cgtttggcgg gaggcatacg aaagcggtcg cagcttcggt
    13681 ttccggaacg ggcagacgac cgtgattgct cctaccggaa cgatcggctt catgatggac
    13741 tgcgacacca ccggaattga gccggaactt gccctggtga agtacaagaa gctcgttggc
    13801 ggcggggtga tcaagatcgt taacaacacc gtgccgcagg cgctgatccg ccttgggtac
    13861 acgccggagc aggtggagcg gatcgtgagc cacatcgatt cgacgggtac catcgaggga
    13921 gcgccgcacc tgaagccgga acatctgccc gtcttcgatt gcagcctccg tccgcagaac
    13981 ggcgcccggt tcattcacca catgggacac ctcaagatga tggccgccgt gcagccgttc
    14041 gtctccggtg cgatctcgaa gaccatcaac atgccggaag agtcgacggt cgaagagatc
    14101 atggaggcct acatcgaggg ctggcgcctc ggcctgaaag ctgtcgccat ttatcgcgac
    14161 ggttcgaagc gggtgcagcc gctgaacacg ggcaaaggcg aaaagaaagc ggtcccgggg
    14221 gcgccggtga ctgagaagat cgtgtaccgc cccgtgcggc ggaagcttcc ggacgagcgc
    14281 caatcgatta cgcacaagtt ctcgatcggc ggccacgagg gctacatcac ggcggggctg
    14341 tacgacgacg gccaaccggg cgagatcttc atcacgatgg ccaaggaggg ctcgaccatc
    14401 tcgggcctga tggacggctt cgccaccgcg atcagcttcg ccttgcagta cggcgtgcct
    14461 ctcaagttcc tggtggacaa gtttagccac gtgcggttcg agccgagcgg ctggacgggg
    14521 aaccagcaga ttccgtacgc gaagtcgatc ctcgactata tctttcgctg gatgggcgcc
    14581 aagttcctcg ggccggagta cacgatcacc gaggccggtg agacgccgaa actgcggcct
    14641 accgaaccgg agccccagca gtcactgccg ttcggcccag cggtggacga cgcaccgctc
    14701 tgcagcgagt gcggcgggtt gatgacgcgc aacggaagct gctacaagtg cgagaactgc
    14761 ggcggcacca gcggttgtag ctaggggttc ttcagccagg acgcgttggt ggggtaactg
    14821 gcggcccggt ccaaatggat cgggccgttt ctttttgtgc ctcctaaata tttctaaatc
    14881 aacaggttgc aacaccggca agtaacggta gactgggaat tatgaagagg gccatctcac
    14941 tctttctctc cgccgcctcc gtgctcgccg cgggcgatgt gctgctggtc ggacacaaat
    15001 gggccgactc cgccgggttc tatgacacgg agacaggaaa gcaactcgcg gtcgttccgg
    15061 cgggcgtcaa accgcacgaa ttcgccctga ccccggaccg ttccgtcgca tacttgacca
    15121 actacggcgt ggaccgctac acggagacca gtccgggcgg caacacgctg acgattctcg
    15181 atctgaggcg caggcagaag gccggtgcga tcgacctcgg gacgttccac cgtccgcacg
    15241 gcatcgaaat gggtccttcc ggacggctct acatcacgtg cgacttcccg ccctcactgg
    15301 tcgtcgtgga tccgaataaa cggaagatcg tcgagagtta tgaactcggc gagtcgcttc
    15361 cccacatgct ggtcctgacg cacgacgaga aggccgctta cacggccaac gccggcgctg
    15421 gaagtgtgac ccttgtccgg ttcggcgcca ggaaaccgga ggcgaggatt cccgtgggag
    15481 gggtgccgat ggggttggcc ttgagccgcg acgggacgcg cctgtacgtt gccactcgga
    15541 ccgcgaacac gattgccgtc attgacactg cgacaaaccg ggtcgtcggg aaaatcgaga
    15601 tcgcgggcaa cccggctcgc cttgcgctga cgccggacgg caagcatctg ctcgcatcgc
    15661 tgatcgaagc gggcgatgtc gcggtgattg acctggcgag ccagcgcgta gctcaccgct
    15721 tccacgccgg cgccaatgtc gaggggatta cggtggaccc gtccggacgt ttcggctacg
    15781 cctcagcgca gggggacaac acagtcgtga agttctctct gagcgactgg aaggaagtgc
    15841 ttcgcatccg gacagagtcc cggccggatc cactggctgt tctccccggc ttctagcaga
    15901 cgctaccggc gcacgtctgt ccgaaccgac cacgagccgt cgcgttcacc gtaagtccac
    15961 gtaccggtga gcgtctcgcg cacggcagac tcgagcagcg tcagaaattc gctggaaggg
    16021 ggaagtttcg tctgtgctgt ccaacttccg gagagcgttc ggccgtccgg aagttgagcc
    16081 agccaggctc ccagccattc ctgcggggat ttccggacgg accaggtgcc gcccgccacg
    16141 acattcccgg agtcgtcctt taggacccag tcgcctccgc acgtgcccgg ctccggctgc
    16201 ggaaaggccc gccaggttcc cctgaacgta cgcttgccgt ccgtggccgc ccagcgaccc
    16261 cctaattcac gcgtctgccc ggcgagtgct tgcgaaagca aacagagccc cagtgcgcgc
    16321 cgtgaaatca cacgcatgag aacagggtag caggtgggtt ggctgaagtg gccctcaggg
    16381 gccgcggatc tccagcatct gaatcacgtg ggtcggacgc tcaggccaac cgagaagctg
    16441 catcatccag cgatcgagcc gggagtaggg aagaaacagg tgggacctga cctgccgtcc
    16501 cgccgagagt tcagtctcca gtttccggtt gatcccgcct ggagacacgt cgtgcgtatt
    16561 gcggaagtac cagacggttc gcgcgcgctg tgtggctacc tcgctcctaa gtgactcaaa
    16621 tgagcggcct ggcgccactt gcaccacacg gacaccgtcc ggcaaccgcg caaccagcgg
    16681 ccagggatcg ttattgtaca tgtccacgat aacgaccgtg tccgttccgg gcgtgcccga
    16741 ctggatcagc cgcgccgctt cgtcgtaggg catcaggtac gccttgttca ggaagccctc
    16801 ccgctggaag tactggatga ttgaggcggc cgacaggcag accaggccgg tcagacagat
    16861 gacgcggagc ctccttccgg cccgctccag gccgagcacc agaagaagca gaaagaacgg
    16921 atagacgaag actaggcgtg ccgggacaaa ggcgaacgag acccaccggc tggccgctag
    16981 ataacctgcc acagcggtga tggccagaag ggcgaaccac accggcggcc gcgcggccgc
    17041 cttccataga cagaagctaa gaagcggggc cagaacaacc gaggcagcta caacccattc
    17101 cgggggcgtc tcgccgaagt gaaaagagaa gaacaggtag gcgagacgga gggcatggct
    17161 cgtaaccgac tctccaaagg gagaataggc ggccgctcga gcgacgcgcc ccatcgccgc
    17221 ctgaaggtag agcagccagg gcaggtacaa aatcacgatc agacctgccg acgccatgag
    17281 tcccccgagg aggcggggct tccgctcacg gactgccttc cacaaaaaga cggcggccac
    17341 tgaggcaagt acggccatgc cgggcaggta gtgtacgtag agcagggcga ggctcgtgag
    17401 gatgaagcca agccaaagcc cgagcttccc ctggccggtg aacaagcggg tggcgaacca
    17461 cagcgccgca cagaagaggg ctgcctggag tgtatacgcc cgtgccatcc gggagtaaag
    17521 cagcatgtag ggcgacaggc accagagggc gagataccaa atacggcagc gcccgggcaa
    17581 gggccgaagc cacaaccggt ccagcagcaa ggtcgccgct agagcaaaca gaacggacag
    17641 cgccctggcc ttgatgggcg tgcttcccgg ccacggccct cggagccaga aatggaccag
    17701 gatgggatgc agcggagggt tgttcacctc cctgcgcacg gtctctaaca cgccggacag
    17761 cggttgagaa gcagtctgaa tcgtgtagtg ttcatctccc caaacgggga gtaagtcgag
    17821 ttgcggaaac agaagaacag cctgaacagc caggcataat ggaagggcgt atcgctcgat
    17881 cgacgcgagg cgcgccaccg cggaattcat gcgaatttcc cactataacg cggagggtgg
    17941 acagggtgta cagcgcgctt caaagtctct caagaaagaa agaagccctg cgtctgggtc
    18001 cgcagggctt cactcagagg ggctggcgac gagctgggcc tcgtcgccgg ggatgctaaa
    18061 gtaatagcat ttagttgtct tgattataca gacaaccgca tccgtttgtc tagacttttt
    18121 tgaaaatttt tttcgagacc ttaagtcttt agttttccgg cagattccga agcaggcggg
    18181 cactttcgta atcgcggacc agttgtttgg cgattcgctc ggcgacatcc gcgctcgcgc
    18241 cccggaattt ccccccccgg ctctcttgcg tcgtggacca cagcacgtcg ccgtccttgt
    18301 cgacgagccg gacagaagcc acagcctcgt gctttcgctc cgcggttcga gtggattcgc
    18361 tctcgccgac agtcacaccc ccgtaccggc tgcggcgatc ggaagaggtt ttatcgggat
    18421 cggtgcccgt gctgagggaa gcgcgggcat tcaggctctc gctcgaggaa aacgtgtcgg
    18481 tgaagatgag gtcttcggcg gagccccgta gaaaagcatc ggcgcgcgcc tcgttttcgg
    18541 tgatcacgaa gagcttggtc gccagcaagc tactgataat catgtcccgg agctgctggg
    18601 cggtatcgcc tcccccgaat cggtccacgt aaacccggcg tacggagagg atgcggacga
    18661 gcgtcgcctg ctctgggctg tcacggcggc tggggagtct ctccggaaga ggcgcctgcg
    18721 cacccccggc aagtacggcg acgactgcgg ctagcatcac gagtttcatt cgacgcctac
    18781 ccctttccct ttcgcgcctc gtcgtcttga tgctgcactt taagtccggt tctcgcttcc
    18841 aggccggcca gcactgcccg aacgtcattc ttgtccaact gaaacacgag tgcctgttgt
    18901 ttcccctgct cgtcggtgaa accgaccgtg agaaagtggt tcctcttttt gctcaacagc
    18961 aacatgggag agatgacgat cgccatggca taacgccggc tggctttctg cccgtactcg
    19021 agcaggttga tgttctcgta cgggatctga accgtcatgg ccttggcctg gaaagaaaag
    19081 cagtcgtcat cggtcatcag gatgaggccc tgaatccggt tgccgacgcc ggcaaccgtg
    19141 cctccgacat aggccacgcg cgctccaggt tctacggcgg ccgagatcgc ggcaaagagt
    19201 gcgcagagaa cggcgagccg tgtggccaga cttccggcgg acatgcgaca tctcctcttc
    19261 tgaatagtgc acaccttcgg caggggctct cactcttccg ccgatcgagg gcgtatgctg
    19321 tgggggagga attcccgctg atgacaagga tctgctttgc attcctagct ttcttattgt
    19381 tttccggacg aggcgtctcc caggagaggc gcgttgttgt gcagccccgg gatacgggag
    19441 ccgcgctggt gaaccccaat atgggttggg tgtttcacca ttacgacaac aacatcacca
    19501 agtacgggct tgagcttgaa ccgtccgata cggtggacga gttccccggc gccagcaccg
    19561 tctacctgcg gcttgcctgg tcgtacatcg agccggaaga ggggaagttc aactggtcca
    19621 tcgtcgatac gcccgcgcag cgctggatcg ccaagggtaa gcaggtggcg ttccggttca
    19681 gttgcgccga atccgaccgg aatcaaccgt atgcgacgcc ggaatgggtc cggaaagcag
    19741 gtgcaaaagg ctacaatttc gtgccgcgca agggagtggt cgcggacggc ccgtggtggg
    19801 aacccgacta cgacgacccg gtgttcctcg agaaactggg tcgcttcctg gccgcggccg
    19861 cagcgcggta cgacggcaac ccggaggtag cattcatcga catcgggtcg atcggcgtct
    19921 ggggcgaggg gcacacatat gcgagcagta tgctgccgtt ctccccggat accgttcgca
    19981 agcacatcga cctccacgcg aggtacttca agaaaacact gatcgccgcg aatgacgacc
    20041 ttgcgcttca tggcaggggg ctcgaagtgc tccattacgc ccggcagcac gggatgactc
    20101 ttcgggacga cagcatcctg gtccagcccg gcgacaaggc gtaccaccgg gcctacctgg
    20161 cgccggtctt ctggccggaa ctccccgtcg tgctggaatc cgagcactac ggtgcgtccc
    20221 gcgacgaagg tacctggcgg gacggcagcc gctacctcga tgccgtcgag gattaccatg
    20281 cgagctgggc gaccgttcac tggtacccgc gcgagttcct caaggaggct cgtcccctga
    20341 tcgacaagat caacatgagg ctgggctacc gtctgcaatt gctggaggca tcctggcctg
    20401 aggaggtgaa ggcgggcgga accatgtcgg ttgggtaccg ctggcgcaac gccggggtcg
    20461 ccccgtgcta tcccggaggc cacccggcga tcacgctgaa agacgcaaag ggcggcattg
    20521 ccggagtttt cgtggacgag gaattcgaca tgcggtcgtt gccggttgga gcccccggac
    20581 aggcgcaccc ggtcgggcgt gaggtcaagg caatctccca agcctcgcgg ccgctgatcg
    20641 actttcggct ccctccaacg aacatcctga agcctggggc gtacgacgta ttttcatcgg
    20701 tcggcacacg gaccggtaca ccgaagatcg cattaccgct tggagcggag gatggccaac
    20761 ggcgatatct tctcggtaca atcaccgtga aatgaaaata ggacaggtgg ttcctttttt
    20821 gttctcggcc gcgttggcgt gcgcagccgc cttctcgcag agccgcgttg tcgtgaagcc
    20881 gcaggatacc ggagccggtc tcaccaatcc cggaatgggc tgggtcttcc accactatga
    20941 caacagcatt cagaattatg gcgtcgatct ggaaccgtcc gacacggtcg atgagtttcc
    21001 gggtgtcggc gttatttact tgaggcttgc gtggtcccac gtcgagccgg aggaaggcaa
    21061 attcaactgg tcggtggtgg acacgccggc gcagcgctgg attgcgaagg gtaagcaggt
    21121 ggccttccga ttcaccgcaa gcgagtccgg aaaggaccag ccttatgcga cgccggaatg
    21181 ggtccgcaag gccggagcga aggggcactt tgtcgatccg agcaagggga tcgtcccggg
    21241 cggtttcatt tgggagccgg actttgacga tccggtattt ttagccaaac tggaccgatt
    21301 ccttgccgca gccgcggcga ggtacgacgg aaacccggaa gtggcgttcg tcgacgtcgg
    21361 ggcatttggc gtctggggcg aagggcacac ctacagcggc tcgctccttc cgtactccgc
    21421 cgctacgatc cgcgcgcata tcgatcttca cagaaagcac ttcaagcaga ccctgctcgc
    21481 cgccaatgac gatttctcac tccagggcag gggaatcgaa actctggact acgcccggcg
    21541 gctcggcctg acgttccgcg atgacagcat cattgtccag ccgggcgaca aagcctggta
    21601 ccacgcctac atggcggact ggttctggcc gcggacgccg gtgatcctcg aatccgcgca
    21661 ttacggctac atgctggaac gcggcaattg gggagatggc agcctctacc tgaaggctgt
    21721 cgaggttcat cacgccagcc tgctgtccgt ccacgggtac ccgaaggtgt tcctgaaaga
    21781 caaccgggcc ctcatcgaca gaatcaaccg ccgtctggga tacaggctcc aattgctgga
    21841 ggcgtcctgg cctgtggagg tgaaggcggg cggaaccatg tcggtcgggt accgctggcg
    21901 caacgccggg gtcgctccgt gctatccggg aggtcacccg gccattaccc tgaaagacgc
    21961 gaagggcggg attgccggcg tgttcgtgga cgaggagttc gatatgcgga cactcccggt
    22021 aggtcctccg gacgaggctc aggccgtggg gcgtgaggtc gaaggctact cgctcgccaa
    22081 tcaggcttcc cggccgatga tcgagttccg gctgccaccg gaccacatcc tgaaggctgg
    22141 ggcgtacgac ctctatgtat cagtcggatc caggagcgga acaccgaaga tcgctctacc
    22201 acttcccggc gacgacggac aacggcgata tcttctcggt aaggtcaccg tccgttagga
    22261 atgccatgcc cggaacggcg caccgccttg acacgctcgc cgtccgggtt taaatttgtg
    22321 accaaaggct gtgtaaggag ggttgccaga tgctggacct taccaggcgg cagaggtccg
    22381 atccggaaga gcggaaggtg atctttctgg agttcgtcgc tgcctccgcc atcttttcga
    22441 tcgtggcctt ggtgctgtat atggtcttca cgtacaagcc tgcctagggc ggccgtccgg
    22501 agggtcgaag agagaccgca cgagcgcaag cgcgtcgtcc cgcgtacgga ttcgagattc
    22561 cagttgagca tcctcgactt cggcgagcat ccggtgaaac gcaggaccgg ggtgatagcc
    22621 tgccgcgatc agatcacgcc ccgtcagtag ccgtggcggc ttcagctctt cagtcttcag
    22681 ttccgagagt ttcgagctga cgaattcata attgtctagc cgccggtggc tgccggcgca
    22741 atccaaccgg tgcaattcca agtgttcgtc aaaatgctcc tgccggagga accgcttaag
    22801 ggtactctcg cgcatgcgcc gcacttccat gaagcgcaga tggtttgcca cgagcacctc
    22861 cacccgccga atctccgccg ccgagaaccg caagcgcgaa aggatctcga cggccattct
    22921 ggcccccact tccacgtggc cgtcgaagcg gatgcgctcc tcaatacgaa aagtgggcgg
    22981 cttgccgacg tcatgcagca ggacgccgag cgcgagcgtc accgtagggt tccgcattgc
    23041 gccgagcatc aacagcgtgt gggtccagac gtcaccctcc ggatggaact caggcggctg
    23101 cgcgaccccc ttcatcgccg caacctccgg caggatctct gccagcaggc ccgattcatc
    23161 caacagctca aagccacgcc gggcgtcgcc ttcgatgagg atgctcacga gttcatcccg
    23221 aacccgttcc gccgagaccc gccgtatcaa cccgcgcagc cgccggatcg cctccatggt
    23281 tccgggctcg atatggaact ccagtgtggt tgcgaagcga acggcgcgca gcatccgcaa
    23341 gtgatcttcg ccgaagcgag tttcgggatc tccgatggcc cgaacacacc tcgcctccag
    23401 gtctgccctg cccccgacga gatcgagcac ctcgccggtt ccggggtcga gcagcagggc
    23461 gttgatggta aagtcgcggc gcaagacgtc cctgcgcggg tctgcctcga actcgaccga
    23521 aacaggacgg cggccgtccc ggtaggcatg gtcactacgg aaagtggcca cctcgacctg
    23581 cgtgtcagcc tccgttacga ccatcacgcc gaagtgagcg cctacctggc gcgagttcgg
    23641 gaacaaggcc aggatctcct caggccgggc gctggtggcg acgtcgtaat ccttaggctc
    23701 acgccccagc agcagatcgc ggacacaccc gcccacaaga tacgcacggt ggccgctcgt
    23761 ctgtagcatc ccaacgatgc gttccgccaa ggcgcccgcg ctggtatccg gcacggccat
    23821 gatcgtagaa taagcgattg ggttcactga gatctcacgt atgtccagcc aggccggtca
    23881 gccggctcct cacgcaaaga tcgtagttgc gacgaccgta gcgttatcgt ttatctcgtt
    23941 ctggcgcggc gccgccatcg ttctgagcga tctcgcttcg tcatcgttct atgccggcgg
    24001 cattgcagag caggccatcg gcaagtcagc gccgtggttc gtgcttggcg tcatgttgtt
    24061 cagctttgcg gtccgctcca tttacatgga aagctgcagc atgtttgtgc gcggcggagt
    24121 gtatgtggtc gtccgcgaca gcatggggcc ggtgatggcg aagctctcgg tttcggcgct
    24181 ggtgttcgac tacattctca ccgggccgat cagttcggtg acggccggcc agtatctggg
    24241 ccacctcctg aacgagatcg ccgggcttgt gaatcagccc taccgcgtgg acccgcactt
    24301 gtttgccgcg gtgttcggtg ccgccgtcac catctacttc tggtggagca acgtcaaagg
    24361 gatccacgag tccagcacga aagcgctccg tattatgcaa atcaccaccg tgatggtggt
    24421 actactgctc atctggtgtc cgattacctt gctgctgaaa gataaggtgg atctgccgcc
    24481 gccgccgacg cccgcgaacc ttcagtttac ggaggagtcg ctcggctggt tccatgacac
    24541 gtcctggcca cagattccgc tcgttgccat catgatcgcc ttcggccact cgtttctggc
    24601 gatgagcggt ttcgagacgc tggctcaggt ctatcgcgaa atcgcttctc cgaaactcag
    24661 gaatctgaag cgcaccgcaa atatcgtctg cggttatgcc gtgatctcta ccggcatcgt
    24721 taccctgctc gcggtaacga tcattccaga tgaggtcagg aagaactatt acgacaacct
    24781 gatcggcgga ctggcgatga acctggcggg accgtatctg ttgcgccttg gattccatgt
    24841 gttcgtcgtc atcgtcggta ttatgatcct gtccggagcg gtcaacacat cgatgatcgg
    24901 cgccaacggc gtgctcaatc gcgtcgcgga ggacggtgtt ctggtggaat ggttccgcaa
    24961 acctcaccac cggttcggca ccacgcatcg tttgatcaac ctggtcacgc tgtttcagtt
    25021 gatcaccatc atcgccagcc gcggcgacgt ctacctgctc ggcgaggctt atgcctttgg
    25081 tgtcgtctgg agtttctccc tgaaaggcct gggcgtgctg gtccttcgct tccagagaaa
    25141 ggaccaggag tataaaacgc cgctgaattt ccacatcgga ggccgggaat ggccggttgg
    25201 actggccatg acgacgctcg ttctgttctt cgtggctgtc gccaacctgt tctccaaacg
    25261 catcgctacc atctacggcg tgtcctttac ggtgctgctt ttcgtggtgt tcacagtgtc
    25321 ggagtatctc aaccggcgga aacggagcgc cgtacacaaa ttaggtctgg aggagttcaa
    25381 tctcgagatc cagccgcagg tgacggcgga gacaatcgag gccaggccgg gctgcgtcct
    25441 cgtagcggtc cgggattaca gccggatggt ccacctccag aaggttctcg aaaagacgaa
    25501 tctgcggcgg cacgacatcg tcgtgatgac ggtgcgtccc gtgacggccg gcgccggcga
    25561 gtacggcctt gccgcgaacc agatcttcag ctcttacgag aaagagcttt tcacggccgt
    25621 ggtctccatg gcggagaaag aaggaaagcc cgtggatttg ctggtggtgc cagccgtgga
    25681 tcctttcgat gcgatggtgc agaccgcttc agaattgcag gcctcccgcc ttgtcaccgg
    25741 tgtatccgcg cgaatggatt cggaggaact ggcgaggctg atcggtcttg cctgggaacg
    25801 gttgcccgag ccaaggcacg ccttttccct cgaagtgatc agtcccgacc ggccgtccat
    25861 ctacgtgaac ctcggtcctc acccgcctcg attgtggccc gaggacattg accggctcca
    25921 tgacatctgg ctgcaacttt ccgagagggc gggctttgga tcgaagctgc atcaccggga
    25981 cgtcgtcggg ttggctctgc gaaggctgca aaagcaactc gacgcgaagg atcgggaaca
    26041 ggtgatcgag gacctgcgca gggaacttgg taagggttaa actgacgggc tggtgccgtt
    26101 ccaacttgat tcacctatgc actacaccca cgttttgtac agcgaaaagg actgtgaggc
    26161 tttcgccgag agcgctatct gaagaccggc agggaaaagc gctaccttcg gtagcggctc
    26221 gctgcgcgtg gcgcgggcct cttagccggg aaaagttgga acggcgctag cttttgccct
    26281 ccggcttgtc cttctgccgt ttgtcggttt ccccctccgt cggatccacc gatgagagca
    26341 cctctttgaa gagggccgct ttcttcagtt ccttcttgga cgggggagcc ttctcgtggc
    26401 ggtagtcgtg gtcgttgtag caggtccggc agcgtacctt tgctggctcg gcgttcaaca
    26461 gcgagacgat cgcgtgattt gtgaggcggc ggcacttgac gcaatagtcg tcaatgacgt
    26521 cgcctaaccg aagatcggac atagcgggtt gtgtctccgc gtttgactcc gcaagtatat
    26581 cgaactcact ggacgacctg ccacaggaga ccggcggcgg cggaaccgac gatcatccag
    26641 acggtgtcga tccgtgtcgc gaccatcagt acaaacactg ctcctgcgag gagcgccggc
    26701 acgactccgg taatcgcgtc ccgggcgagg gggacgcttg cagagaggat cagtcccgcc
    26761 gcggccagcg tcacgctctg aatcacctgt ttcgcccgcg gatggtcggc ccgacgccca
    26821 aagtgacgga ccatcggcac gatgaggaac gcaggtgtca tgagtgcaag catgcccgcg
    26881 aaagcgccgg ggacacccgc cacgaagtag cctacgctga cgacgtacaa cccgagtggt
    26941 ccgggcccca tgcttccgac cgccaccgcg gtgttcagtt cccggtccgt gagcacgcga
    27001 tgctggacta ccaggtcgtt tctcagcatg ggcagagatg caaggccgct gaacgatgtc
    27061 attgttgcct tcagcagaat caggtagagg atgacgacgt tcatcgtttc gccggttccg
    27121 gccaaagcaa acccgtgatc gccgccagcg ccagtactgg aatcggtgat aaggagaagc
    27181 cgagcgacag gatgagtgct gccgcgacaa gcgcccacgg ccgcagccgc gaactcccgg
    27241 ccgcctgcga acggatgagg agccacgccg ccgcggccat cattccgacc gctgccgcca
    27301 caatgccgtt gaacgcgcct accgccgccg gatgcgcccg caattgctga tagctgtggg
    27361 tgaggataac ggctagcaca gcggaaggaa ccgtgactgc ggccacggcc gcgaaggcgc
    27421 ctggccagcg ccggatgaac cagcccgccg ctgcgcaaaa cgcgagcaga ttcgtccccg
    27481 gcgtgattcg cgcgagtccg taggagaggc cgtactggtc ggccgaaatc cagcctttcc
    27541 tgctcaccag ttcacgctgg agcgcggcca tggtcggctc gcctccgccg aaggtgagat
    27601 tgccgattcg aagcgcgatc gagaacaatg tgcgaagact tggcatttcg ggcttgctga
    27661 cggatcagta cgggtcggag ctggcttggc gcatcgcctc ggcctgattg gcggtgatga
    27721 ccgcgacgtt atagatgtcc tccgccgagc aaccccggga caagtcgtta ccggctttcg
    27781 ccagcccttg cagcagtggg ccgatggccg tggcgccagc cagccgctcc accaatttgt
    27841 aggcgatgtt agcggcctcg aggttgggga agatgagcgt attggcgcgg ccggccaccg
    27901 tggagccggg ggctttcgaa cgcccgacag cttctacaag cgctgcgtcc gcctggagtt
    27961 cgccgtctgc gtggaggttc gggtcgcggg cccggatgat ccgtagcgcc tcggccactt
    28021 tgtccactgc cttgtgcgtc gcgctcccct tagtcgagaa cgacagcagg gccactgccg
    28081 gttccacgcc aagcaggttg cggacgcttc cggcggtagc gatggcaatg tccgccagtt
    28141 gcaccgcgct cgggtcgatg acgatcgagc agtcggcgaa gaacagcagg cctttgtgcc
    28201 cgaaagcgcg gtcgtgcagg gccatgatgt ag
//
LOCUS       DATIFQ010000306         2386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_675597, whole genome shotgun sequence.
ACCESSION   DATIFQ010000306 DATIFQ010000000
VERSION     DATIFQ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675597"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VLE22_14450"
     CDS             complement(<1..651)
                     /locus_tag="VLE22_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB15656.1"
                     /translation="MSLGRPGLDELVPSRYALRVGEIDVMVVSDGVLLLPGAMLGHNA
                     DPAVRAAWLNDMFLPPDVFEWALNAVVVRSGSRTVLIDAGIGVDPGLNLPRAGRLVQR
                     LEAAGIDLASVTDVVLTHMHMDHIGGLLVDGVKDRLRPDLRIHLAAAEAKFWESPDFS
                     RTAMPPDMADLIRHAAKRFLNEYQGQLRPFEKEYEVAPGVLVTRTGGHTPGHSVVRL"
     gene            complement(799..1647)
                     /locus_tag="VLE22_14455"
     CDS             complement(799..1647)
                     /locus_tag="VLE22_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB15657.1"
                     /translation="MSTITTKDGVKIFYKDWGKGQPIVFSHGWPLSADDWDAQMMFFV
                     NQGYRVIAHDRRGHGRSAQVSDGHDMDHYADDLAALTAHLDLKNAIHVGHSTGGGEVT
                     HYLGRHGESRAAKAVLIDAVPPIMVKTPANPGGLPMEVFDGLRKSLADNRAQFYLDFA
                     SGPFYGFNRPGVKPLPGVYQNWWRQGMMGSAKAHYDGIKAFSETDFTEDLKKITVPVL
                     VMHGDDDQIVPYADSAPLSVKLLKNGTLKTYKGFPHGCPTTQADIINADLLAFLRSGL
                     ATKTAA"
BASE COUNT          478 a          727 c          712 g          469 t
ORIGIN      
        1 caggcgcaca acgctgtgcc cgggggtgtg gccgccggtg cgagtgacga gcacccccgg
       61 cgccacctcg tactccttct cgaacggccg cagctggccc tggtactcgt tcaagaaccg
      121 cttggccgcg tgccgaatca agtccgccat atccggcggc atggcggtac gggagaaatc
      181 gggcgactcc cagaacttgg cctcggcggc cgccagatgg atccgcaggt ccggacgcag
      241 ccggtccttc actccatcga cgagcagccc gccaatgtgg tccatgtgca tgtgggtaag
      301 caccacgtcg gtcacggacg cgagatcgat gccggcggcc tccagtcgct gaaccaaccg
      361 cccggcccga ggcaagttta agcccgggtc cacccctatc ccagcgtcga tgagtacggt
      421 ccggcttccg ctacgcacca cgaccgcgtt gagcgcccac tcgaacacgt ccggcggcag
      481 gaacatgtcg ttcagccagg cggcccgaac ggccgggtcg gcgttatggc ccaacatcgc
      541 gcctggcagc aacagaaccc catcgctcac caccattacg tcaatctcgc cgacccgcaa
      601 cgcgtagcgc gacggaacca actcgtcgag ccccggtcta cccaagctca tgtttgtctc
      661 tgctgcggta gtgctgcgcc gggtagctaa tgaagattgt gcggacatga tcgttctccc
      721 tcgagggaaa tgctcttgga cgtaggtaag caagccgaga ggaactgtat gatcttcccc
      781 tcggcttttc ggtcgccgct atgctgcagt tttggtcgca agtccggaac gcaggaaggc
      841 cagcaggtcg gcatttatga tgtccgcctg ggtggtgggg cagccgtgcg ggaagccctt
      901 gtaggtcttt aacgttccgt tctttagtag cttgaccgac aatggcgcgg agtcggcgta
      961 gggaacgatc tgatcgtcat caccgtgcat gaccagaacg ggcaccgtga tcttcttgag
     1021 gtcctcggtg aagtcggtct ccgagaacgc cttgatgccg tcgtaatgcg ctttggcgct
     1081 ccccatcatg ccctgccgcc accagttctg atagacgccc ggaagtggct tcacgcccgg
     1141 tcggttgaag ccataaaagg ggccgctcgc gaaatcgaga tagaattgcg cccggttgtc
     1201 ggcgagcgac ttgcgcaagc cgtcgaacac ctccatcggc agaccgcccg gattggccgg
     1261 cgtcttcacc atgattggcg gcaccgcatc aatcagaacc gccttggcgg cgcggctttc
     1321 gccgtgccgt cccagatagt gggtaacctc gccgccgccg gtggaatggc caacgtgaat
     1381 tgcattcttc agatcgagat gcgcggtcag cgccgccaga tcatcagcat agtgatccat
     1441 gtcgtgaccg tcgctcactt gtgcggaccg gccatgacct cggcggtcat gagctatgac
     1501 gcgatagccc tgattgacaa aaaacatcat ctgcgcgtcc cagtcgtcgg ccgacagagg
     1561 ccagccatgg ctgaacacga tgggttggcc cttaccccaa tccttgtaaa agatcttgac
     1621 gccgtctttg gtcgtaatcg tactcatggg tacctccgtg gctggaaatt gacctttccc
     1681 cagcaccgca aagttgcccc ggagccttcg agcaccctga attgtcgcgt aggttgttgg
     1741 ttgttaccgg gaacgatgaa gtgccgtctt tgttcccgac taagcaagat gatgactggt
     1801 cagtaatgct gacgattacc ttccatgtcc gctttcagcg accaaaaaag cggacacgcg
     1861 cctcgcaaga ggtgatatct ggcgcgtgat ccattcagca atgggattgc gaaaggcgct
     1921 ttggcctgaa cctaaagcga tggactccaa ggttccgcga gcaggaaatg ccaggttcgg
     1981 cgcgtgcggc cactgccaat tctcgaggta gagaccggaa cttcgattca tctgggaaaa
     2041 agatgagcgg gcggatcgtg tcgcgccgaa tcggcacatc gatgcgaggt cggatcctta
     2101 cctgaggcga cgggcgcagg cggcggcaat cgagggccgg tccagaagtt tgaggtattc
     2161 gtcgatcgga acataggtgg agctgcggcg caactagcga tgtcggctat gggccataaa
     2221 cggacatcag ttttccagca aagccactgt gccgagccca ccatcggcac agatactaac
     2281 gatcgcccga cttcccttgg gcattgttgc aagttccttt accgcctgac ttaggattcg
     2341 cgccccggta gcagcgaagg gatgaccgag tgcgacacta ccgccg
//
LOCUS       DATIFQ010000307         4211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2591428, whole genome shotgun sequence.
ACCESSION   DATIFQ010000307 DATIFQ010000000
VERSION     DATIFQ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591428"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VLE22_14460"
     CDS             <1..418
                     /locus_tag="VLE22_14460"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSB15658.1"
                     /translation="YINIRLANERLVAYLSSFFGILALGLASLGLSGVLACVVTQRTR
                     EIGIRMALGARRGDVVRMILRFSLVPVVAGLVIGVVVAFSWGLFLGSLIYGIDTFDLV
                     SVSQSVAVLLAAALLAAALPARRATKVDPMVTLRYE"
     gene            1207..1461
                     /locus_tag="VLE22_14465"
     CDS             1207..1461
                     /locus_tag="VLE22_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15659.1"
                     /translation="MLAFRRYRWVFLLAVVAVGAFLLLSAINSASGHFEFVALLPLLL
                     VDIGSLVAIGSYVTGEEPVRHAFPLVSNLVPRAPPTLSVS"
     gene            1538..1921
                     /locus_tag="VLE22_14470"
     CDS             1538..1921
                     /locus_tag="VLE22_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15660.1"
                     /translation="MFLLADNDAVQALRRFGTINIETLEQFMIAALVFFGGIVTFIFF
                     LKRLLAWMDERGWITYTGHVPTYRSVGHAFLELQSLVEPQKEYVLELKEDEEQKREED
                     DEAGPDDPTRGIARHARIEKEETHE"
     gene            complement(1960..3015)
                     /locus_tag="VLE22_14475"
     CDS             complement(1960..3015)
                     /locus_tag="VLE22_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15661.1"
                     /translation="MKILRTVSGLAVLVGICALRVAVAAEPVPLKVTASWNIPMTEEG
                     VRAAVAGAKELGFNGYAWSSQRLDEALVSQCRASGMRCFKLLSPLAKRPGARVQVVEE
                     AERRLPGFAGGLTSEYQYGGEPLPGHPEVLNLELMCPLDEGVVPHVASEVARAKRLGY
                     HGVCWDFVGYRNYHSCECETCRAALKAFLRNPSNGSPRAAKAAFYEKALVDLYERLYD
                     VTRKAAPQFTVMTHCHPVFLPNVFHGLKLKVDYSASTVGWFFQPHWSLEKVSDYARRT
                     VRGPYRFQSAIGMPMIGCYSSGAMAQHRKSGKRLRAEFHAVREAGARAVMVCELGDIF
                     SDPEIREAVRAGLAEIR"
     gene            complement(3108..>4211)
                     /locus_tag="VLE22_14480"
     CDS             complement(3108..>4211)
                     /locus_tag="VLE22_14480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB15662.1"
                     /translation="QAPAPDASQPTPDELLLKDYRPQPIFKIPKTDIKKAKYPVIDVH
                     SHGPRPIEQLPEFVKTMDAANVEKTVILTGANSVERFAEVSRAYSSYPGRFELWCGFD
                     LTGFGEPRFGPNAVKALEECHRLGAVGVGEVSDKGWGFRTSAARRSAVGATPAGQRPA
                     PGPGPHPDDPRIDALWDKCAQLGMPVNIHVSDPIWAYEPMDKTNDGLPNGYKWRIDDK
                     QPGIFGHNALIESLERTLEKHRKTTFVACHLVNLGYDLTRLGQMLERHPNLYLDISAR
                     FGELAPIPRASAEFFQKYPDRSLYGSDMAYSQRMFTSTFRILETLDEHFYEFDLDVCY
                     DYHWPLHGFGLPDAVLKKMYRDNALEVFRRARS"
BASE COUNT          828 a         1258 c         1261 g          864 t
ORIGIN      
        1 atacatcaac atccgcctgg cgaacgagcg cctggtagca tacctctcga gttttttcgg
       61 aatcctggcg cttggcctcg cctcgctggg gctttccggt gtgctggcct gcgtggtgac
      121 gcaacggacg cgggaaatcg gcatccgcat ggcgctcgga gcacggcgcg gagacgtggt
      181 caggatgatt ttgcgcttct ccctcgtgcc ggtggtggcc ggactggtga ttggggtcgt
      241 ggtggcattt tcctggggtc tcttcctcgg cagcctgatc tacggcatcg acactttcga
      301 tcttgtgtcg gtgtcgcaat ccgtcgccgt cttgctggcc gctgcgctgc tcgccgccgc
      361 cctccccgcg cgccgggcga ccaaagtcga tcccatggtg acgctgaggt acgaatgagc
      421 tcatcccgag tctcaaaggg aatgtcgaac gcggatcgca gcgtttatga ggagcggctc
      481 ttaacttaag attgcgatcc gaggtatagc cgtgggcata gcaggaatca tgggatcgat
      541 gctctccttc atgcaggacg cgtccgtagt gaaacaacat tgggagagtt ccctaaagct
      601 gactcgccaa acaagtattt ggtccttggt cggacgggac ttgttcgagc atgccgtgat
      661 tggccttgtc tgttgggcag tgttgctcat ctttgcacca aggggcttgc ttcggccttt
      721 gatcctacga ttcgttgatc aatgctgtgg cggctgaata ctgtgaaagc atggttatac
      781 gtagtaacca cgtggaatca gtaggttcgc gggaattccg cgtgaaaagc ggcctcttgt
      841 ggaatcaaca agtcaccgtc gcgtataacc atcgccaacc gcaaaaatgc cgcaaccgcg
      901 caaggcttgc gcctgcaata ggttactgtg gggtgatacg tcacccgagc ggggaactta
      961 gggcgagttt cggtgacaag tttcagtttc ggtgacaggc tacgcaatcg ccaaacttac
     1021 tgagcccgtt gtggcgatac ccgctgcgcg atcgcggcgg cgcgggcggc ggaccggtga
     1081 gtgtgggggg attcgtagcc tgtcaccgga actaccggaa ctcacgttga aacaggccaa
     1141 gctgaaacag gcccacgctt gactccggtg atctggccgt gctatttgtt agcgcgggta
     1201 cagtagatgt tggcgtttcg caggtaccgg tgggtcttcc tcctggctgt agtagccgtc
     1261 ggcgcctttc tgctgttgtc cgcgattaac agcgcgagtg ggcatttcga gttcgtcgcg
     1321 ctgctgcctc ttctgctcgt cgacatcggc agtttagtgg ctattggctc ctacgtcacg
     1381 ggtgaagagc cagttcgcca cgcctttcct ctcgtcagca atttggttcc ccgcgcacca
     1441 cccactcttt ccgtctcgta gccggttttg ggaggaggcc gtgcgccccc tccgattcct
     1501 gaagaaagct ggcaattgga acgaaaagga gggttttgtg ttcctactag cagacaatga
     1561 tgccgtgcaa gcgctgaggc gcttcggaac gataaacatc gaaactctgg agcagttcat
     1621 gatcgcggcg ctcgttttct tcgggggcat cgtgacattc atcttcttct tgaagagatt
     1681 gttggcatgg atggacgagc gcggctggat cacctacacc ggccacgtac ccacttaccg
     1741 gtcagtcggc cacgcgtttt tggaactgca gtccctcgtc gagccccaga aggagtacgt
     1801 gctcgaattg aaggaagacg aggaacagaa acgggaagag gatgatgagg caggtccgga
     1861 cgatccgact cgagggattg cccgacacgc aaggatcgag aaggaggaaa cccatgaata
     1921 gcggcttggg tctgttcagg cctgtggtag tcgcaacgcc tatcggatct ccgccaggcc
     1981 cgcacgcacg gcctcgcgaa tctcaggatc agagaaaatg tcgccaagct cacagaccat
     2041 caccgctcgg gcgccggcct cccgcacggc atggaactcg gccctcagcc gcttgccact
     2101 cttccgatgc tgggccatcg cgcccgagga atagcagccg atcatcggca tgccgatcgc
     2161 cgactggaag cggtaaggcc cccgcacggt ccggcgcgca tagtcactca ccttctccag
     2221 actccaatga ggctgaaaga accagcccac cgtgctggcg gaatagtcca ccttcagctt
     2281 cagaccatgg aagacattcg gcaggaagac cggatggcag tgggtcatca cggtaaactg
     2341 cggcgcggcc ttgcgggtga cgtcgtaaag ccgttcatat aagtcaacga gcgccttttc
     2401 gtaaaatgcc gccttggccg cccgcgggct gccattcgaa ggattacgca gaaacgcctt
     2461 cagcgccgcc cggcaggttt cacactcgca ggaatgatag ttgcgatagc cgacgaaatc
     2521 ccaacacacg ccgtggtagc ccaaacgctt cgcgcgagca acctcgctgg cgacgtgagg
     2581 gacgacgccc tcgtccaaag ggcacatgag ttccaggttc agcacttccg gatgtccggg
     2641 cagcggctcg ccgccgtatt gatactcgct ggtcaagccg ccggcgaaac ccggcagccg
     2701 ccgctccgcc tcctctacta cctgcacccg cgccccggga cgcttcgcga gcggcgacag
     2761 caacttgaaa catcgcatgc cgctggctcg gcactgcgac accagcgcct cgtcgagacg
     2821 ctgggaactc caagcatacc cgttgaaacc gagctccttc gccccagcca cggccgcgcg
     2881 gaccccttct tccgtcatgg ggatattcca gcttgccgtc accttgagcg ggacgggctc
     2941 ggccgcaacc gccacacgca acgcgcaaat cccaactaag accgccagac ccgacacggt
     3001 tcgcaagatc ttcatcaccg cctcacgttc catagtcctc gaggccagac gaatcggcga
     3061 agcaggaatc gagtaacgga cgagtcgtct ggtcgaacgg gcgtccgtta ggaacgtgcc
     3121 cgccggaaca cctccagcgc gttatcgcgg tacatcttct tcaacaccgc gtcaggcagg
     3181 ccgaagccat gcagaggcca gtggtagtcg taacagacgt ccaggtcgaa ttcgtaaaag
     3241 tgttcgtcga gtgtctcgag gattcgaaac gtactggtga acattcgctg cgaatacgcc
     3301 atatccgatc cgtaaagcga acggtcgggg tacttttgga aaaactccgc cgaagctcgc
     3361 ggaatgggcg ccagttctcc gaaccgggcc gagatatcca ggtatagatt cggatgacgt
     3421 tcgagcatct ggccgagccg tgtcaggtcg tagcccaagt tgacgagatg gcacgcgaca
     3481 aaggtggtct tgcggtgttt ctccagggtg cgttcaagac tctcgatcag ggcgttatgc
     3541 ccgaaaatcc ccggctgctt gtcgtcgatg cgccatttgt agccgttcgg aagcccgtcg
     3601 ttcgtcttgt ccatcggctc gtaagcccag atggggtccg aaacatgaat gttgacgggc
     3661 atcccgagtt gcgcgcactt atcccataga gcgtcgatgc gcggatcgtc cgggtgcggc
     3721 ccgggacccg gcgccggcct ctgcccggcg ggcgtggcgc caaccgcgct acggcgcgcc
     3781 gccgaggtcc ggaagcccca tcctttgtca ctgacttccc cgaccccaac ggcgcccaga
     3841 cgatggcact cttccagggc tttgactgcg ttcggcccga atctcggctc accgaagccc
     3901 gtcaaatcga agccgcacca caactcaaaa cgtccggggt aactggagta ggcccggctg
     3961 acttcggcga acctctcgac cgaatttgcg ccggtcagga tcacggtctt ttccacgttg
     4021 gcggcgtcca tcgttttgac aaactcggga agttgttcga tgggccgcgg cccgtgcgaa
     4081 tgaacatcaa tgacgggata cttcgccttc ttgatgtccg tcttcgggat tttgaaaatg
     4141 ggctgggggc ggtaatcctt gagcaagagt tcgtccggcg taggctgcga cgcgtccggc
     4201 gccggggctt g
//
LOCUS       DATIFQ010000308         6694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2205900, whole genome shotgun sequence.
ACCESSION   DATIFQ010000308 DATIFQ010000000
VERSION     DATIFQ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205900"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VLE22_14485"
     CDS             complement(<1..297)
                     /locus_tag="VLE22_14485"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HSB15663.1"
                     /translation="MKLSVQVLAFLFASLSSAQTPCQPTAVYTPCDIVFDMTDEEARA
                     HPNPYLTVQLQAEFRSPRYRTFLMPAFWDGGRRLVIRFAPTDPGKWDFRISSNIA"
     gene            complement(336..1475)
                     /locus_tag="VLE22_14490"
     CDS             complement(336..1475)
                     /locus_tag="VLE22_14490"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HSB15664.1"
                     /translation="MHWQSLVDFLVLAAVFYAVLRWARQTRALRVILWIAGVQAASRV
                     AGWLDLVITSWVLSGVSVLLVVLLLFGFQSELRSALLRLDSLVRLGLKPAVKAFLPAN
                     RAIAGAAFRLASERTGALIVIARNDSVRELVSGGVPLGAEITPELLSALFRKDSPLHD
                     GAVIVEAERVVRAGAVLPLTHRQEVPAMFGTRHRAAMGLAERSDALVVAVSEERGAVT
                     LMHGQSIVTLTSSVGLADRLGELRTQPPAGRPGAIRRLLFQDLRYKFAALGMAVAVIA
                     LSVLSTGTTVRAISVPVEFVNVPPGMAISSQSPSRVEAQVRGSRWLIDSVTDSGLVAR
                     VDLRRAGQGIQDVRVRPDALNVPPGVVVERVTPPGITVRLVRKLP"
     gene            complement(1552..2451)
                     /locus_tag="VLE22_14495"
     CDS             complement(1552..2451)
                     /locus_tag="VLE22_14495"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HSB15665.1"
                     /translation="MRSLFAAFITCFTLLNVGWTQPSQPESALCVDGLVAPAVSGGLA
                     GGIKKPELTGVDPVDLVRRSAQADKENARRGSNYAFRAHHYQRDLDASGRPKGWHSRT
                     WDIIGLEGSTYRKLVMINDHPLTAKRQKREDERLRKETERRRNEAPDKRKNRLLHYSV
                     ALPFDRFSEIYTFTLLGEETVAGRSAWVIDGIPKPEFRPQTNAEKEVANFHVKIWIDR
                     DEAQPQALELTVTGGHSRLQKGTTARSEWTRLNDGTWMLKRTNITWGVRLFRVITLRG
                     TTEDTNSDFQRFQVDSQLVIDES"
     gene            2605..4710
                     /locus_tag="VLE22_14500"
     CDS             2605..4710
                     /locus_tag="VLE22_14500"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HSB15666.1"
                     /translation="MRSACLLCVCVAATVAQTYSVRTDRISIEYDERFHQHIRWLGDR
                     GGNIVAFDPAAQESAEVNGWRCSEFRIDPKSVSSRRTQDTEFGPALEGTLTGEFRDSE
                     KGIHIERRVRVVLPDKLPDVAVFEKSYRNLGEKPVRLGPVYSQRIVLDRRLANPGEPS
                     WAFASFQGGAYRWGLDYSLIWLRPEFRQSNFQGLDDRTGPEGEGGGIPFVDVWSPTMG
                     VAIAHLEKTPQWVSLPVEVRADGKTEIGVWEKPEARFKQQEWLAPGEAFRTVMTAVIF
                     HHGDYYDALHAYGNLLRARGVAILETSPPSAYKPYWKSWGFGFDFTQDKILGLLPELK
                     EMGIELANLDDGWFDYYGDWQVNRSPGKFPGGDPDMKRFVRKIHDGGFRTAIWWYPLG
                     VSTDSRLAKEHRELLVQSENGGYPTDDRKVHQLCPAYEPALRHIAETLKRFITDWDFD
                     GVYVDSVGLTAVPPCFNTAHGHRLPLDSFQALPRMYKTIHDELYRLKPDPYLEVCICA
                     MPHSPYNMPYYPIANASDPVSLAQVRQRVKLEKAIRGPRFCVGDCYQVPLDEWQGSSV
                     PESFETAMGTGAQLTTFYADLTGDRRAKWRRWFHLYNELGLASAEYLNLYDIAFDKPE
                     IHVVRKGADLYYGIFADVWGKNRRIELRGLDKALEYEVYDYADGIPLGKVKGNDPYIR
                     APFKDALLVRVRPIPRTAP"
     gene            complement(4658..5653)
                     /locus_tag="VLE22_14505"
     CDS             complement(4658..5653)
                     /locus_tag="VLE22_14505"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSB15667.1"
                     /translation="MAKRVRVLSIDGGGIRAIIPALVLARIESETGKSVAELFDLIAG
                     TSTGGILALALTKPGPDGRPAFLARELVGLYEEEGERIFHRDVWHRMRALGNLAEEKY
                     PANGIEAVLDHYFGNARLKDAATDVLVTSYETERRLPFFFRSSRARTRADYDFPMKLV
                     ARATSAAPTHFEPLKLAADGAADYYSLIDGGVYANNPAMCAFVEAKTLYPGAEDFLVV
                     SLGTGELTRSLRFDDIKDWGLGKWAQPILDVVFDGVSSTVEYQLRQLLTPETGPRCYW
                     RFQVRLDDETCSMDDAQPENIRRLRLFAEQLIRDRAEDLRALCAELAARAPEARP"
     gene            complement(5837..>6694)
                     /locus_tag="VLE22_14510"
     CDS             complement(5837..>6694)
                     /locus_tag="VLE22_14510"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15668.1"
                     /translation="QCLSAGIHVLVEKPLDVSVEGCREMIAAADSGGLLLQVDFHKRY
                     DPYHRELHDMAASGCFGEILYGYAYMEDRIEVPRDWLRKWAAQTTPAWFLGVHLFDLI
                     RWITGSEAQSVSATGVKKKLVSLGIDTYDAIQAKLVLRSGASFSVDVSWVLPEGHEGV
                     VNQGIRVTGTDGMMEVDTQDRGARCCLRKPTDGRPQQGSGMQTPNLGFFREGRDKLGR
                     PRYSGYGIESIQDFAENVSRLIGGGSLTDLHGTYANGRDGLEASRIAVTVHRSMEAGG
                     ALVDVSRTL"
BASE COUNT         1338 a         2092 c         1993 g         1271 t
ORIGIN      
        1 ggcgatgttg ctggaaatgc ggaagtccca cttgcccggg tcagtcggcg cgaaccggat
       61 caccaggcgc cggccgccat cccagaacgc cggcattagg aaggtgcgat aacgtggtga
      121 gcgaaactcg gcctggagct ggactgtcag gtaggggttc ggatgcgccc gagcctcttc
      181 atccgtcatg tcgaacacga tgtcgcaggg cgtgtaaacc gccgtcggct ggcacggagt
      241 ctgggccgaa ctgagtgagg cgaacaggaa cgccagaacc tggacagaca gcttcatgag
      301 aacagtgtag cgggagcagc atctccgcgg cgtctttaag gcagctttcg cacaaggcgg
      361 accgtgatgc cgggaggagt aactcgctca accacaacac cgggtggaac gttcaacgcg
      421 tccgggcgga cgcggacatc ctgtatgccc tggccggccc gccgaaggtc gacccgcgca
      481 accagacccg aatcggtcac cgagtcaatg agccaccggc tacccctcac ctgggcttca
      541 acgcgactcg gcgactggct cgagattgcc attcccggag gaacgttcac aaactcgacc
      601 ggaacactga tcgcccgtac ggttgtgccc gtcgagagaa ccgacagcgc gatgaccgcc
      661 acagccattc ccagagcggc gaacttgtag cgtaagtcct gaaatagtag ccgtcgaatg
      721 gccccgggcc gccccgcggg cggttgggtc cgcagctcac ccagccgatc cgccaaccct
      781 acggacgagg tgagtgtgac aatgctctgc ccgtgcatca acgtgaccgc cccacgctcc
      841 tctgagactg caaccacaag tgcgtcactt cgctcggcga ggcccattgc cgcccgatgc
      901 cgcgtcccga acatggctgg cacttcctgg cgatgtgtga gtggaaggac ggcgccggcg
      961 cgcacgaccc gttctgcctc gacgatcact gcgccgtcgt gcagcggcga gtcctttcgg
     1021 aagagggcgc tcagcagctc gggtgtaatc tccgccccca gcgggacgcc cccgctgacg
     1081 agctcgcgga ctgagtcgtt acgggcaatg acgatcagag ctcccgttcg ttcggacgcc
     1141 aagcggaacg cagccccggc gatcgcccgg ttggccggca aaaacgcctt caccgccggc
     1201 ttcaatccaa gacgcaccag gctgtcgaga cgaagaaggg cgctccgaag ctccgattgg
     1261 aatccgaata gcagcagcac caccagcaga acactcacac cactaagcac ccaacttgtg
     1321 atgacgaggt caagccatcc ggctacccgg gaggcagcct ggacgccggc gatccaaagg
     1381 atgacccgca gcgcccgcgt ctgccgcgcc cagcgcaata ccgcgtagaa cacggctgcc
     1441 agcaccagaa aatcaaccag gctctgccag tgcatccagg gcatcttgag aggagcatgg
     1501 tcgcatcagc cgcctcctcg ttgcacgaag ttgggcacgg aacacggaca cctatgactc
     1561 gtctatcacc aattgcgagt ccacctgaaa ccgctgaaag tcagagttag tatcctcagt
     1621 cgtgccgcgc aaggtaatga ccctgaaaag gcgaacgccc caggtgatgt ttgtccgctt
     1681 cagcatccag gtgccgtcgt tgagcctagt ccattcgctc cgcgcagtgg tacccttctg
     1741 aagacgggag tggccaccgg tgacggtgag ctccagcgcc tggggctgcg cctcatcccg
     1801 gtcgatccag atctttacgt ggaagttcgc gacctccttt tcggcgtttg tttgaggccg
     1861 aaactcaggt ttagggatgc catcaatgac ccaggcagaa cgacccgcga ctgtttcttc
     1921 accgagtaac gtgaatgtgt agatttcgga gaacctgtca aacggcaggg cgacactgta
     1981 gtgtaatagc ctgttcttgc gtttgtctgg cgcctcattt cggcggcgct ccgtttcctt
     2041 tctaaggcgc tcgtcctctc gcttctgccg cttcgccgtc agagggtggt cattaatcat
     2101 cacgagcttg cggtaggtag atccctccag gccgatgatg tcccaggtcc tggaatgcca
     2161 gcctttaggc ctgcccgaag cgtccagatc tctctgatag tgatgcgcgc ggaaggcgta
     2221 gttggatccg cgtctggcgt tttccttatc ggcctgagcg gagcggcgca ccaggtcgac
     2281 aggatctacc ccggttaact ctggcttctt gatgccgccc gcgagccctc cgcttacggc
     2341 cggggcgacg agcccatcca cgcagagtgc agattcaggc tgtgatggct gggtccaccc
     2401 cacattgagc agtgtgaagc acgttatgaa ggctgcgaac agtgagcgca taaagcctta
     2461 gacgcagcat aagtatatga gttgcgaata tcttattttc gtttgtcgcc gatgccggcg
     2521 ccgacccgga cagcaaacgg ctactgagaa cgttgacaat gcacgcccat tccccagtgg
     2581 tgataacctg tggccggagt gcccatgaga tccgcctgtc tgctgtgcgt ctgcgtggcc
     2641 gccacagtgg cccagaccta ttccgtccgt accgaccgca tttctatcga gtacgacgaa
     2701 cggttccacc aacacatccg ctggcttggc gacaggggcg ggaacatcgt tgcgttcgat
     2761 ccggcagccc aggagtccgc cgaggtgaac ggctggcgat gctcggaatt ccgaatcgat
     2821 ccaaagagcg tctcgtctcg gcggacacag gacacagagt tcggaccggc gctcgaaggc
     2881 acgctgactg gcgaattccg agacagcgag aaagggattc atatcgagcg ccgagtccgt
     2941 gtggtgctgc cggacaagct gcccgacgtt gccgtcttcg aaaagtcgta ccggaatctc
     3001 ggcgagaagc ccgttcgcct cggccctgtc tacagccagc ggatcgtgct cgaccgcaga
     3061 ctcgccaacc ccggagagcc atcgtgggcg tttgcctctt tccagggtgg cgcctaccgc
     3121 tggggtcttg actattcgtt aatctggctg cggcccgagt ttcgccagtc gaactttcag
     3181 ggactcgacg accgcaccgg gccggaaggc gaaggtggcg gcattccgtt cgtcgacgtc
     3241 tggagcccga cgatgggcgt ggcgatcgca cacctcgaaa aaacgcctca gtgggtctcg
     3301 ctgccggtgg aggtgcgcgc ggacggcaag accgaaatcg gagtctggga gaagcccgaa
     3361 gcccgcttca aacaacagga gtggctcgct ccgggcgaag ccttccgcac ggtgatgacg
     3421 gcggtcatct tccatcacgg agactactac gatgcgctgc acgcgtacgg caacctgctg
     3481 cgcgctcgag gggttgcgat cctggagact tcgccgcctt cggcctacaa gccgtattgg
     3541 aaaagttggg gcttcgggtt cgacttcaca caggacaaga tcctagggct gctcccggaa
     3601 ctgaaagaga tggggatcga actcgccaac ctcgacgacg gctggttcga ctattacggc
     3661 gactggcagg tgaaccgttc gccgggcaag tttcccggcg gcgatcccga catgaagcga
     3721 ttcgttcgaa agatccacga tggcggcttt cgaacggcta tctggtggta tccgctgggt
     3781 gtgagtaccg acagccggct cgcgaaagag caccgcgagt tgctcgtgca atcggagaac
     3841 ggcggctatc cgacggatga tcgcaaggtc caccagttat gcccggccta cgaaccggcg
     3901 cttcgccata tcgcggaaac gctgaagcgg tttatcaccg actgggactt cgacggagtc
     3961 tatgtggaca gcgtcggcct gactgccgtg cctccctgct ttaacacggc ccacgggcac
     4021 cgcttgccgc tcgattcatt ccaggcgctg cccaggatgt acaagaccat ccacgacgag
     4081 ttgtaccgcc tgaagcccga tccctatctg gaggtctgca tctgcgccat gccgcactcg
     4141 ccgtacaaca tgccttacta tcccatcgca aatgcttccg atccggtgag cctggcgcaa
     4201 gtgaggcaga gagtgaagct tgagaaagcg atccgcggcc cccgcttctg cgtcggcgac
     4261 tgctaccagg tcccgctcga cgagtggcag ggttcctcgg tccccgaatc gttcgagacg
     4321 gcgatgggca cgggcgcaca gttgacgacg ttctatgcag atctgactgg cgacaggcga
     4381 gccaagtggc gccgctggtt ccatctgtac aacgaactgg gacttgcctc cgccgagtat
     4441 ctgaacctgt acgacatcgc cttcgataag ccggaaatcc acgtcgtgcg aaaaggcgcg
     4501 gacttgtatt acgggatctt cgcggacgta tggggaaaga accgccgtat cgagttgcgc
     4561 gggctggaca aggcgctcga atacgaagtc tacgattatg ccgacggaat cccgcttggc
     4621 aaagtgaagg gtaacgaccc gtatatccgc gcgcccttca aggacgcgct tctggtgcgc
     4681 gtgcggccaa ttccgcgcac agcgccctga ggtcctccgc gcgatcccgg atgagctgtt
     4741 cggcgaacaa cctcaggcgg cggatattct cgggctgggc atcgtccatc gagcaggtct
     4801 catcgtccag tctcacttgg aagcgccagt aacatcgcgg accggtctcc ggtgtcagta
     4861 actgtcgcaa ctggtactcc actgtgctgc tgacgccgtc gaagacgacg tcgaggatcg
     4921 gctgggccca cttgccgagt ccccagtctt ttatgtcatc gaaccgaagg gagcgcgtca
     4981 gctctccggt gccaagcgag accactagga agtcctctgc gccggggtag agtgtcttcg
     5041 cctcgacgaa cgcgcacatg gcgggattgt tggcataaac gccgccatca atgagcgagt
     5101 aataatcggc cgctccgtcg gccgcgagtt tgagcggctc aaagtgggtc ggcgcagccg
     5161 atgtagcccg tgccaccagc ttcatcggga agtcgtaatc ggctctcgtt cgcgcccggc
     5221 tgctgcggaa gaagaacggc aagcgccgct cggtttcgta actggtcacg agcacgtccg
     5281 ttgccgcgtc ctttaggcgg gcgttgccga agtagtgatc aagcacggct tcgatgccgt
     5341 tggcgggata cttctcttcc gccagattgc cgagggcgcg catcctgtgc catacatccc
     5401 ggtggaagat gcgctctccc tcctcttcat agagacccac taattcgcgc gccagaaacg
     5461 cgggtcttcc atccgggcca ggcttcgtta gggccagcgc tagaatccct ccggttgagg
     5521 ttccggcaat caggtcaaag agttcagcta ccgattttcc ggtttcactt tcaattctgg
     5581 cgagaaccag cgcggggata atagcgcgga taccccctcc atcgatggac agaacccgaa
     5641 ccctcttagc cacgccgccc cccttgcctg gaatgtagca tccaaacata agcagtgcgt
     5701 cgcaaaagca ccctgcaagc gttcgaatcc gaaacgaatg tccttgtctg ccaagcagtt
     5761 acagttacat gtgggagcag gaggcgttct gtgaacaaaa ggagggtgga tctttcgatc
     5821 agccccttcg gaaatatcag agtgtccggc ttacatccac aagcgcccca cctgcttcca
     5881 tgctcctatg caccgtcact gcaatccggc tcgcctccag tccgtcccgc ccgttggcat
     5941 aagtgccatg caggtccgtg agcgaacccc ctccgattag gcggctgacg ttctccgcga
     6001 aatcctggat cgactcgatc ccgtacccgg agtatcgcgg tcttcccagc ttgtctcgac
     6061 cttcacggaa gaagccgagg ttcggcgtct gcattcccga gccttgctga ggacggccgt
     6121 cggttggctt ccgcaggcag cagcgtgcgc cgcggtcctg ggtgtccact tccatcattc
     6181 cgtccgtgcc agtcacgcgg atgccttggt taaccacgcc ctcgtgccct tcgggcaata
     6241 cccaggagac gtcgaccgag aaactggcgc cgctacgcag caccagcttt gcctgaattg
     6301 cgtcgtacgt gtcgatgcca agcgacacaa gcttcttttt cacgccggtc gccgacacag
     6361 actgagcttc cgagccggtg atccaccgaa tcagatcaaa caggtggacg ccgagaaacc
     6421 aggccggcgt tgtctgagcg gcccacttcc gaagccagtc gcgcggaacc tcgatccgat
     6481 cctccatgta ggcgtagccg tacaggattt cgccgaagca gccgctggcg gccatgtcgt
     6541 gcaattcgcg gtgataggga tcgtaccgct tgtgaaaatc cacctggagc agcaggccac
     6601 ctgagtcggc cgcggcgatc atctcccggc agccctccac ggaaacgtcg agtggtttct
     6661 cgacaagtac gtgtatcccg gcactgaggc attg
//
LOCUS       DATIFQ010000309         6034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2782944, whole genome shotgun sequence.
ACCESSION   DATIFQ010000309 DATIFQ010000000
VERSION     DATIFQ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2782944"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VLE22_14515"
     CDS             <1..311
                     /locus_tag="VLE22_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15669.1"
                     /translation="FSESVKVQAPGWLTARCSSTLGPSLSARFGIAAHTSPVYLRVAG
                     EEQFSEPALTYLLTLVEGTRGYVEKLATRPDTERFARVLKVFAEAREHLHRRLHEQGR
                     "
     gene            392..2089
                     /locus_tag="VLE22_14520"
     CDS             392..2089
                     /locus_tag="VLE22_14520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15670.1"
                     /translation="MNHCLKLFGLLFVFATLAATDQPVVYEGGVVNAASYGSDWLQNG
                     GVAQGSMFVVFGSRMGPAVLQMATTFPLQLKLANTSIEVTIAGVMVNAIMLYAWDRQL
                     AAVLPSRTPVGNGTLQVVYNDLRSDPVTIRVVETNFGSFAGTQAGSGPAIAQNYVSAT
                     ETPLNSPIEAAHPGQVMILWGTGLGAVSFDETFPPLVQDLNVPLEVWIGGKRATVLYE
                     GRSPQFPGIDQINLQLPADAPLGCYVPLAVKARGVTSNFTSIAISPSGKICSDPHGVS
                     AADLNRLAAGEPLRLGWIQLMRENFKAQVPGIGAVEGSFDLGWADFFRWDASSVIRSH
                     ALSSWGQTALGTCTEITFEEEPNFAPIHPSRLVVGETMNLHGPLGIKPMPRDRAGLYE
                     KNAIGTNIPPVLGWSNKTPFLEPGTYTVENGSGGPDATRFSVSLTIPHDPASLQWTNL
                     ESIQIIDRSRDLTVTWEGGNPDREYVLVGAMSSVGGLENAKAGVIVACAERVSAGRLV
                     MPSFVLSTLPPSDPETAASMFVVIRAALPESSTTVAGGLDAIYSMAAEGHIRLVTYQ"
     gene            complement(2199..2504)
                     /locus_tag="VLE22_14525"
     CDS             complement(2199..2504)
                     /locus_tag="VLE22_14525"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HSB15671.1"
                     /translation="MPCRGGVPPLEGEELLRLSAQLPDWKVIEQHHIRKTFLFPDFRK
                     ALDFVNRVGEVAEEQGHHPDLALSWGKVDVEIYTHKINGLTESDFILAAKIDRVFFQ"
     gene            complement(2528..2872)
                     /locus_tag="VLE22_14530"
     CDS             complement(2528..2872)
                     /locus_tag="VLE22_14530"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSB15672.1"
                     /translation="MALWKLARAAEIPPGQTRFVWAGEAAVLLVNYRGAIYALSGICP
                     HRNNPLEGAVLWGHLVDCPYHHFQYDVRTGENHFPKNVYPEDMPQLEQQVRPLRSYRV
                     EVREGDVWVDLG"
     gene            complement(2892..3458)
                     /gene="dps"
                     /locus_tag="VLE22_14535"
     CDS             complement(2892..3458)
                     /gene="dps"
                     /locus_tag="VLE22_14535"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF009990.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HSB15673.1"
                     /translation="MAKVARQVVEQAGIDVNVLLDKLLRAAGAEFTTFYYYTILRVNS
                     IGFDGEGLKEIIEDARIEDRNHFEALTPRIYELGGALPRDIRQFADIAGCPDAYLPAD
                     PRNMSAMLAVLVEAERCAVRTYTDICNMTFGKDHRTYDLALAILHEEIEHEAWFSEYL
                     SEGPSGHFRRGSPGTSPYVRRFMSAEIG"
     gene            complement(3446..3883)
                     /locus_tag="VLE22_14540"
     CDS             complement(3446..3883)
                     /locus_tag="VLE22_14540"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HSB15674.1"
                     /translation="MPRILDSFRTNALRFSPQRYAVLQYLTTHPAHLTAEEVHKALNR
                     NHPRASLATIYKSLHALAQAGLVREVNLSANVTRFECAGERHHHFVCERCTRIEDLPW
                     FDVAGLAVKTKVAPRTVRTWELVLYGLCESCQPQPKEKLKWRK"
     gene            complement(4086..4511)
                     /locus_tag="VLE22_14545"
     CDS             complement(4086..4511)
                     /locus_tag="VLE22_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15675.1"
                     /translation="MCDYSLMGLPSRLAVEREELVVHRFSTGTVGLASPGDLTTMPIN
                     RVPAVCIAPGAHLLLQDIPPFLQRTLGVQDTEPVTFTQITAKTNSHRDAFRFGNGREI
                     LLQELIGGQRVWVLDIPSGDNPRPDAAEAERERVFAGMF"
     gene            4776..5060
                     /locus_tag="VLE22_14550"
     CDS             4776..5060
                     /locus_tag="VLE22_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15676.1"
                     /translation="MIVVRNTFTAKPGQASKLAAQLKEMASAGNLPKHRVLTDVTGEF
                     NRVVLEYQVESAAAFEEMFKKYTTDPQIREKTKGYTDLWFTGSRELWRVV"
     gene            complement(5074..>6034)
                     /locus_tag="VLE22_14555"
     CDS             complement(5074..>6034)
                     /locus_tag="VLE22_14555"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15677.1"
                     /translation="WMSSIFGVPPHWFNTTYWAEFDPKVPGLVWGAFSRTHDLPRPKM
                     WRRANPDTFQGGIGWSCDGGDHWIIAQGLPETAVTHLLLDPTSPVGSRTLYAAAFGRG
                     VYKSNDNGKTWALRNAGIDAKQPFAWRLTRAENGTLYVVIARRSENKIGDWEDGALYK
                     STDGAEHWTKMKLPAGANGPTGLTLDPRDNNRMYLSTWGYQTPEGDTGGGVYLSTDGG
                     GTWKHIVTEQQHVYDVTVDPKNPDVVYHCGFDAGAWRSADRGATWKKVKGFNFKWGHR
                     VVPDPYDPSKIYITTFGGSVWHGPAQGDPNAVEDIITPVKIER"
BASE COUNT         1187 a         1842 c         1813 g         1192 t
ORIGIN      
        1 cgttctcgga atccgtgaaa gtgcaggcgc ctgggtggct gacggcgcgg tgttcgtcga
       61 cgctggggcc ttccctgtcg gcacggttcg ggatcgcggc gcacacatcg ccggtttatc
      121 tgcgtgttgc aggagaagag cagttctccg agccggctct tacgtacctc ttaacacttg
      181 tggaaggaac tcggggctac gtggagaagc tggcaactcg ccctgacacg gagagattcg
      241 cacgagtgtt gaaggtgttc gcggaagcac gtgaacacct ccaccggcgg ttgcacgagc
      301 agggccgata gggcgaaggg agcccaaaga agatattccg gtcgtatctg ctgcttttgc
      361 cttataatca gccccgggga aaggcgcaga catgaaccac tgtctgaagt tgttcggact
      421 cctcttcgtc ttcgcgaccc tggccgcgac agaccaaccg gtggtttacg aaggcggtgt
      481 tgtgaacgcc gccagctatg gttcggactg gctgcaaaac gggggcgtcg cccagggctc
      541 catgttcgtc gtgttcggca gccgaatggg tccggccgtc ctgcaaatgg ctacaacgtt
      601 cccgctccaa ctcaagctcg ccaacacttc catcgaggtc accatcgccg gggtgatggt
      661 gaacgcgatt atgctgtacg cgtgggaccg gcaactggcg gcggtccttc cttcgagaac
      721 gccagtgggc aacggcaccc tgcaagtggt ctacaacgat cttcgaagcg acccggtaac
      781 aatccgggtg gtggaaacga acttcggcag cttcgccggg acccaggccg gaagcggtcc
      841 ggccatcgcc cagaactacg tatccgcgac cgaaacgccg ctaaactccc cgatcgaagc
      901 ggcccatccc ggccaggtga tgatcctgtg gggtaccgga cttggcgccg tcagcttcga
      961 tgagaccttt ccgccgctcg tgcaagacct gaacgtccct ctcgaagtct ggatcggcgg
     1021 caagcgggct acggtgctat atgagggacg ctcaccgcaa tttccgggga tcgatcagat
     1081 caatctccag cttccggctg acgcgccgct gggatgttac gttcctttgg cggttaaagc
     1141 ccgcggcgtc acgagcaact tcacctccat cgctatctcg ccttcaggga agatctgttc
     1201 ggatccgcac ggcgtctcgg cagccgactt gaatcgtctc gctgccggcg aaccgctgcg
     1261 tttgggctgg atccagctca tgcgtgagaa tttcaaggcc caagttcctg gaatcggagc
     1321 agtggaagga agcttcgatc tcggatgggc agacttcttc cgctgggacg caagttcagt
     1381 catccgttcg cacgctctct cgtcgtgggg acagaccgct ctcggaacct gcacggaaat
     1441 aacgttcgaa gaagagccga atttcgctcc gatccatccg tcccggctgg tcgtcggaga
     1501 gacaatgaac ctgcacggac cgttgggcat taagccgatg ccgcgtgata gagccggcct
     1561 gtacgagaag aatgccatcg gaacgaacat tccaccggtg cttgggtggt ccaacaagac
     1621 tccattcctg gagcctggca cctataccgt ggagaacggt tccggggggc cggacgcgac
     1681 gagattcagc gtttcactca cgattccgca cgatccggca tcgctgcagt ggacaaacct
     1741 cgaatcaatc cagatcatcg accgctcgcg ggacctgacg gtgacctggg agggaggaaa
     1801 cccggaccgt gagtacgtgc ttgtcggagc gatgtcctcc gtaggaggcc ttgaaaacgc
     1861 caaggctggc gtcattgtcg cctgcgcgga acgggtctca gccggacgcc ttgtcatgcc
     1921 gtcgttcgtc ctttcaacgc tgccgcccag cgatccagaa acggctgcat ccatgtttgt
     1981 cgtgattcgc gccgccctgc ctgagtcctc gacgacggtc gcgggcggac tcgacgcgat
     2041 ctactcgatg gcagccgaag ggcacatccg gctagtgacc taccagtagt gtgcacatag
     2101 cggcgattgc gagtctgcgt caccgcgtga gcacctccac cggcggctgc acgagcacgg
     2161 cgcccgcgcc ggcggcaagg taggcgcgag gcccatcgct attggaaaaa cacgcggtcg
     2221 atcttcgccg ccaggatgaa gtcgctctcg gtcagcccgt tgatcttgtg cgtgtagatt
     2281 tctacgtcga ctttgcccca ggaaagcgcc aggtccgggt ggtggccctg ttcctcggcc
     2341 acttcgccca cgcgattgac gaaatccagc gccttgcgaa aatccggaaa gaggaacgtt
     2401 ttccggatgt ggtgttgctc gatcaccttc cagtcgggca attgagccga cagccgcagc
     2461 aactcctccc cctcgagggg aggcactccg cccctgcaag gcacgcatct cttatcggca
     2521 agagcgccta ccccagatcc acccacacgt ccccctctct cacttccacc cggtaagacc
     2581 gcagaggccg gacctgctgc tccaactggg gcatgtcctc cgggtacacg ttcttcggaa
     2641 agtggttctc gcccgttctg acatcgtatt ggaaatggtg gtagggacaa tcgacgagat
     2701 gtccccacag caccgcccct tccaacggat tgttcctgtg cgggcagatg ccactcagcg
     2761 cgtagatggc gccccgataa ttgaccagca gcacggcagc ctcaccggcc cagacaaacc
     2821 tggtttgccc gggcgggatt tccgccgccc gggccagttt ccatagcgcc actcgttccg
     2881 tccagcgtgt ctcagccgat ctccgccgac atgaatcttc gaacgtacgg agacgtcccc
     2941 ggagagccgc ggcggaagtg gcccgagggt ccttcggaca ggtactccga gaaccatgcc
     3001 tcgtgttcaa tctcctcgtg gaggattgcg agagcaaggt cataggtacg gtggtctttg
     3061 ccgaaggtca tgttgcagat atcggtgtac gttcgcacgg cgcatcgttc ggcctcaacg
     3121 agcacggcga gcatcgccga catgttcctc ggatccgccg gcaggtaggc gtccggacag
     3181 ccggcaatgt cggcgaactg ccgaatgtcc cgtggcagcg cacctcccag ttcatagatc
     3241 cgcggggtga gggcttcaaa atggttgcga tcttcgatgc gcgcgtcttc gatgatctcc
     3301 ttgagtccct cgccgtcgaa gccgatcgag ttcacgcgga ggatcgtgta gtagtaaaag
     3361 gtggtgaatt ccgcccccgc ggcacgcagc agcttatcga gcaacacatt gacatcaatc
     3421 ccagcttgct cgacaacttg tctcgctact ttcgccattt gagtttctcc ttgggttgag
     3481 gttgacagga ctcgcacaag ccgtacagga caagttccca ggtccgcacg gtccgcggag
     3541 ccaccttggt cttcactgcg agcccggcga cgtcgaacca cggaaggtcc tcgatccgcg
     3601 tgcagcgctc gcagacaaaa tgatgatgac gctcgcccgc gcactcgaag cgggtgacgt
     3661 tggcggacag gttgacttcg cgaacaagtc ccgcctgcgc gagggcgtgt aaagacttgt
     3721 agatcgtcgc caaagaagct cttggatgat tgcgattgag ggctttgtgg acttcttccg
     3781 cggtcagatg agcgggatgc gtggtcaggt actggaggac ggcataccgc tggggcgaga
     3841 atcggagagc attcgtccga aaggagtcca gaatccgcgg catccctcac ccccagccga
     3901 tggctgtaga atacttctca atagagaatt agtcaagggt tccggtggac cgttccctgc
     3961 aatcgtggct cggaaaaatg gacgggcgac ctccccccgg cacccgcgcc gattcactgc
     4021 gggcggattg ggaaaaggcc accctcgcag gccaccaaaa acgatggcct acgccacaaa
     4081 ctacgctaga acatgccggc aaacacgcgc tcgcgctctg cctcagccgc gtcaggtctc
     4141 gggttgtctc cggagggaat atccaacacc catacgcgct ggcccccaat gagctcctgg
     4201 agaagaatct cgcggccgtt cccgaagcgg aacgcgtcac ggtggctgtt tgttttcgcg
     4261 gtaatttgcg tgaacgtgac cggttccgtg tcctgaacgc ccaacgtgcg ctgcaggaac
     4321 ggcggaatgt cctgcaacag cagatgggca ccgggtgcaa tgcagacagc gggaacccgg
     4381 tttatcggca ttgtcgtgag gtccccaggg gatgcaagcc caaccgtgcc ggttgaaaac
     4441 cggtgcacga ccaactcctc cctctccaca gccagccggc tcgggaggcc catgagcgaa
     4501 tagtcacaca ttgtcctttc ctcctcgtcg gttcttgaaa caggccacgg aaggcaacaa
     4561 cgtgagattg aggttgggcc gctcgccgat gaatcgtccc agagtagcga ccagcccgcg
     4621 acggagtatg gacaggtgaa cgtttcggaa attgagtgaa cttctgcctg cctcaggaac
     4681 tctaatgaca tcgtacgccc acccgatccc tggcgcaagc atgatcgaga agggaaagta
     4741 cacatggtag gattcgttta gaggaggctc aactcatgat cgtcgttcgc aacactttca
     4801 ccgccaagcc gggtcaggcg agcaagctgg ctgcgcaact gaaggagatg gccagcgccg
     4861 ggaatctccc gaagcaccgt gttctcacgg acgtaaccgg cgaattcaat cgcgtcgtgc
     4921 tggaatacca agtggaatcg gctgctgcgt ttgaagagat gttcaagaaa tacaccacgg
     4981 acccgcagat tcgcgaaaag acgaagggct acaccgacct ctggtttacc ggcagccggg
     5041 agttgtggcg ggtcgtctga ccacttctcg cggttacctc tcgatcttta ccggcgtgat
     5101 gatgtcctcg acagcgttgg ggtctccctg tgccgggccg tgccagacgc tgccgccgaa
     5161 ggtcgtaatg taaattttgg acggatcgta cggatccgga accacacggt ggccccactt
     5221 gaagttgaag cccttcacct tcttccaggt cgcgccgcgg tcggcggacc gccaggcgcc
     5281 ggcgtcgaat ccgcagtgat acacgacgtc ggggttcttc ggatcgaccg tcacatcgta
     5341 gacgtgctgc tgctcggtga cgatgtgctt ccatgtcccg ccaccgtccg tcgagaggta
     5401 tacaccgcct cccgtgtcgc cctcgggcgt ctgatagccc caggtgctca agtacattcg
     5461 gttgttgtcg cgggggtcga gcgtgagacc ggtgggaccg ttggcgcccg cgggcagttt
     5521 catcttcgtc cagtgctccg ctccgtcggt tgacttgtag agcgcgccgt cctcccagtc
     5581 gccgatcttg ttttcgctgc gcctcgcgat aacgacgtag agcgtcccat tttccgcgcg
     5641 tgtcagccgc catgcgaaag gctgtttggc gtcgatgccg gcgtttctca gcgcccacgt
     5701 ctttccattg tcgttggatt tgtagactcc cctgccgaaa gcagcggcgt agagagtccg
     5761 tgacccgaca ggactagttg gatcaaggag gaggtgcgtt accgcggtct cgggcagacc
     5821 ctgtgcaatg atccagtggt cgccgccatc acaggaccat ccgatgccac cctggaacgt
     5881 atccgggttg gctctccgcc acatcttcgg ccttggcaaa tcgtgcgtac gggagaacgc
     5941 gccccagacg agcccgggga ctttcggatc gaactccgcc caataagtcg tgttgaacca
     6001 gtgcggcggg acgccaaaga tggaactcat ccag
//
LOCUS       DATIFQ010000310         2991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2017866, whole genome shotgun sequence.
ACCESSION   DATIFQ010000310 DATIFQ010000000
VERSION     DATIFQ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017866"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VLE22_14560"
     CDS             <1..255
                     /locus_tag="VLE22_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15678.1"
                     /translation="WYKAMRGRSTEEGHGGPDYLELIRFVEAVRAGAPTPIDVYDSVT
                     MSAIIPLSEQSIDKGSAPVPCPDFTRGKWKTKKPYFALEG"
     gene            complement(271..729)
                     /locus_tag="VLE22_14565"
     CDS             complement(271..729)
                     /locus_tag="VLE22_14565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HSB15679.1"
                     /translation="MGSNAPTSCPKLLADYPVVIHMPVEWGDQDPFRHVNNIVYLRWC
                     ETARVVYLQRIGMWRMIAEENKGPILAALSCNYRRPVEFPDTVHIGARITRIGNSSFG
                     MEHLIVSETMNEAVADADSTLVFFDYAANQPLPLPDDLRHAIQTMEGKEL"
     gene            779..1690
                     /locus_tag="VLE22_14570"
     CDS             779..1690
                     /locus_tag="VLE22_14570"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSB15680.1"
                     /translation="MRAWILLAALLPLAAQETSERPARRQEMERRATETLARFQGTVC
                     FFAKNLDTGESFGIRENDRVRTASTIKLPVMAAVYAAVAEGRVRFADELVLQKENTVS
                     GSGVLTEFTPGVRLPLRDVMHLMIVVSDNTATNMVLDKVPGDYVNEQMEKLGLKETRS
                     LRKVLGEAASAPRGISRAGQVSENKRYGLGVSTPREMVMLLEKLERGEVVSKQASKEM
                     IEVLKRQQYKDGIGRTRKLPVASKSGALDHLRSDVGIVYSQQGRIAIAITCDDIPKID
                     WSHENPGEVLMADLTQILIDGLGKQAP"
     gene            1687..2499
                     /locus_tag="VLE22_14575"
     CDS             1687..2499
                     /locus_tag="VLE22_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSB15681.1"
                     /translation="MKIALVQMECHWGKIDRNLKEMDRHCGEARSFGAKMAVFPELTT
                     TGIFKDDRVWEVSENLDGASVRQVSEMASRHKLYIGFGFSERAEPLPYNAYAVVAPDG
                     AVAGVYRKNCIPRLEVPWWQGHDARPVFEVAGRRCGVAICWDTTQPALLAEYGRNGAE
                     ILLMPHAWDADPLGLDGQDLPHESMDELYEHFREGRLGGWRSHDGMLDQFLRYIPARA
                     RENRFYALFVNQCGRPHEVLCINGPTFAVDPQGETLVSTRDGREQMLLVDIP"
     gene            2596..>2991
                     /locus_tag="VLE22_14580"
     CDS             2596..>2991
                     /locus_tag="VLE22_14580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB15682.1"
                     /translation="MRELALTMTVAAEASESPEPIALLRLAKAGDAAAFEQLIVLHER
                     RVFLIALRLLRRREDAEDAAQEVFLRLHKHLRRFDDTREFPPWLYRVTVNVCRDLARS
                     RQRDWAVEVGEQGHVADPTAEYERREHKRI"
BASE COUNT          577 a          911 c          976 g          527 t
ORIGIN      
        1 tggtacaagg ccatgagggg gcgatccacc gaggaagggc acggtgggcc ggactacctc
       61 gagctcatcc gcttcgtcga ggcggtgcgc gccggcgccc cgacgccgat cgacgtctac
      121 gactccgtca cgatgagcgc gatcattccg ctctcggagc agtcgatcga caaaggcagc
      181 gcacccgtcc cctgtcccga cttcacccgc ggcaagtgga agacgaagaa accgtacttt
      241 gccctggaag gatgaggcgc cggagaggcc tcacaactcc ttgccctcca tcgtctggat
      301 agcgtggcgg aggtcgtccg gtaatggcag cggctggttt gccgcgtaat cgaagaagac
      361 gagcgtagaa tcggcgtccg cgacggcttc attcatcgtt tcgctgacaa tcaggtgctc
      421 catgccgaag ctgctgttgc cgattcgggt gatcctcgcg ccgatatgga ccgtgtcggg
      481 aaactccacc gggcggcggt aattgcagga aagcgcggcc aggatggggc ccttgttctc
      541 ttccgcgatc atcctccaca tgccgatgcg ctggaggtac acgacccgcg cggtctcgca
      601 ccaacgcagg tagacgatgt tgttcacgtg gcggaagggg tcctggtcgc cccactcgac
      661 cggcatgtgg attaccaccg gataatcagc tagcagcttc ggacaggacg taggcgcgtt
      721 cgaccccatt tcttcaggag tagcacaaca cgcccccgcc gtacgagcat agtacgttat
      781 gcgtgcatgg atcttgctgg ctgccctgct cccgctcgct gcacaggaaa cctcggaacg
      841 tccggcgagg cggcaggaga tggagcgtcg cgcgacggag acgcttgcgc ggtttcaggg
      901 gaccgtatgc ttcttcgcga agaacctcga cacgggcgag agcttcggca tccgcgagaa
      961 cgaccgcgtg cggacggcga gcaccatcaa gctgccggtc atggccgctg tctacgcagc
     1021 cgtggcggag ggccgcgtac ggttcgccga tgaactggtg cttcagaagg aaaataccgt
     1081 ctcgggttcc ggagtgctca cggagtttac tcccggcgtc cggctgccgc tccgagatgt
     1141 gatgcacctg atgatcgtcg tcagcgacaa caccgccacc aacatggttc tcgacaaagt
     1201 gcccggggac tacgtcaacg agcagatgga gaagctcgga ctgaaggaga cgagatcgct
     1261 gcggaaagtc ctcggcgagg cggcctccgc gcctcgcggc attagccgcg ccgggcaggt
     1321 ctccgagaac aagcgctacg gcctcggcgt gagtacgccc cgcgagatgg tcatgctgct
     1381 cgagaagctc gagcggggcg aagtggtgtc gaagcaggcg tccaaggaga tgatcgaggt
     1441 cctcaaacgc cagcagtaca aggacggcat cggccggacg cggaaactgc ctgtggccag
     1501 caagtcgggg gcgctcgatc acctccgcag cgacgtcggg atcgtatact cgcagcaagg
     1561 ccggatcgcc attgccatta cgtgcgacga tatcccgaag atcgattggt ctcacgaaaa
     1621 cccgggcgag gtcttgatgg cggatttgac tcagatcctg atcgacggtc ttggaaagca
     1681 ggcgccatga aaatcgctct cgttcagatg gagtgccact ggggcaaaat cgaccgcaac
     1741 ctcaaggaga tggaccgcca ctgtggcgaa gcacgcagct tcggcgcgaa gatggccgtc
     1801 ttccctgagt tgaccacaac gggcatcttc aaagacgacc gtgtctggga ggtgtcggag
     1861 aacctcgacg gcgcgtcggt gcgccaggtg agcgagatgg cgtcccggca caagttgtac
     1921 atcggcttcg ggttctccga gcgcgctgaa ccgcttccct acaacgccta cgctgtcgtc
     1981 gcgccggacg gagccgttgc gggcgtctat cgaaagaact gtatcccgag gctcgaagtt
     2041 ccgtggtggc aagggcatga cgcgcgcccg gtattcgagg tcgccggcag gcgttgcgga
     2101 gttgccattt gctgggacac gactcagccc gccctgctgg ccgaatacgg gcggaacggc
     2161 gccgaaatcc tcctgatgcc acacgcgtgg gacgccgacc ccctcggcct cgatggccag
     2221 gatctcccgc atgaaagcat ggacgagttg tacgagcact ttcgcgaggg ccggctcggc
     2281 ggctggcgca gccacgacgg catgctcgat cagttcctac gctacatccc cgcccgagcc
     2341 cgggaaaacc gcttctatgc gctgttcgtg aatcagtgcg gccggccgca cgaggtgctg
     2401 tgcatcaacg ggccgacctt cgccgttgac ccccagggcg aaacactcgt ctcgacgcgg
     2461 gacggccggg agcagatgct gctggtcgac attccctgac gaaacgcgag aaacgagctg
     2521 gttaaccgct tcccaaaaat ctgtttgcac gttttgccgg ccggaggagt atatctgttt
     2581 tggataccga atccggtgcg ggaactggcg ctcacaatga cggtggcggc tgaggcatct
     2641 gagagccccg agcctatcgc cctgctccgt cttgccaagg ccggcgatgc ggcggcgttc
     2701 gaacaactga tcgtccttca cgagcggcgg gtgttcctta tcgcgttgcg cctgctgcgg
     2761 cgccgggagg acgccgagga tgctgcgcag gaggtgttcc ttcgcctgca caagcacttg
     2821 cggaggtttg acgacactcg tgagttccca ccgtggcttt atcgggtgac cgtgaatgtc
     2881 tgccgggacc tcgctcgttc tcggcaaaga gactgggcgg tggaagtggg cgagcagggg
     2941 cacgtcgcag acccgactgc ggagtacgaa cgccgcgagc acaaacgcat c
//
LOCUS       DATIFQ010000311         6761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1251648, whole genome shotgun sequence.
ACCESSION   DATIFQ010000311 DATIFQ010000000
VERSION     DATIFQ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251648"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VLE22_14585"
     CDS             <1..368
                     /locus_tag="VLE22_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15683.1"
                     /translation="EKGTDRIVIGPVPDPQIMGKRMKRRSAGTGVQLNDASGNERGGL
                     AVIDDGSMVVGIDDEAGRERAHLYFIPKKGSGLLLQGESGKQKISLAVPPEGEQSASP
                     QLEMTDRAGNRVAAIPTRK"
     gene            898..1054
                     /locus_tag="VLE22_14590"
                     /pseudo
     CDS             898..1054
                     /locus_tag="VLE22_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468104.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1114..2118
                     /locus_tag="VLE22_14595"
     CDS             1114..2118
                     /locus_tag="VLE22_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15684.1"
                     /translation="MPVANGKAGGPAELVRQNTGRLRPIGFTAEGTLYYSLENRGSDI
                     YSADLGPDTSKAAGAPKPLIERFMGKNANPVWAPDGKRLAYYSMRGHPPTMGVLSIRN
                     EETGEEVDLYPKLAWFKGLSWFPEGRSLLAYGVDLTNTMAYFRIDAETGEVNKLFDVD
                     FRGLVPWAVLSPDGKRLYNLGPRGLVEHDLANGGERAVARPPKGAEFVELAMSPDGKF
                     LATKLNKSPYSLAILPVDTGEVREVLTIPPPNWFLSIAWTPDSRYLLSAKQFEVENER
                     AELWRVAVATGEAQSLGLSFQSIRDLSIHPNGRRIAFTYAHRTSEIWAMENLGTGSAR
                     "
     gene            2244..3092
                     /locus_tag="VLE22_14600"
     CDS             2244..3092
                     /locus_tag="VLE22_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15685.1"
                     /translation="MNQRPIAAFVLLVCTGGIAVAQSGPHVRFQAHAATGVEEPDRRM
                     TYGLNITPPWEDGGFLFVNLPEHLEYMPGTKGIARHHDPRPNVWRVSEDGDRASYDVE
                     SQTEPGVFLRCEARAEAQRARFELTITNRSRKTLKSIRPLLCYQYHHLKGFPAARTDN
                     FAHTFVVSGGKPVPVSALPVKRPEAYARMAQVAGCPDEHNWWAERMGGFIEPRLDTAI
                     TVLTASDDDRKIAVRWRPGKNLLTNSAIPCIHADPCIGDLKPGESRTVSGDVIFMRAP
                     LEDLIR"
     gene            3117..4358
                     /locus_tag="VLE22_14605"
     CDS             3117..4358
                     /locus_tag="VLE22_14605"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HSB15686.1"
                     /translation="MKILVFGLGSAGDVHPNAGLGLALQERGHRVVLAAASVFEPLAR
                     RLGLDFAGAGTEGEYNDAIQDPDLWHPWRSFNVVANRLMLPWMRKTYEMIANAWESGR
                     IVVAAPATALGARIAQEKLGVPLATVHLQPSMLRSLVQPSCYGFPDIIGALPHWLREP
                     YMRLVDRQIIDRPLARSVNAFRAELGLPPVRRLFNEWIHSPELVIGMFPPWFAPPPPD
                     WPPNVHLTGFPLYDEREARQIQPELEDFLRMGEPPIVFTAGSAMAQGKEFFSVSAEVC
                     RIRGWRGILLTQYPEQLGTLPDSVRHFNYVPFSTVLPRAAAFVHHGGIGTTAQALAAG
                     VPQLVVPFAHDQPDNAVRVRRLGTGDYVRPKDYQANRVVRKLEALMGSTDIRENCRLR
                     AEALANSTALNSACELIERLD"
     gene            4497..5681
                     /locus_tag="VLE22_14610"
     CDS             4497..5681
                     /locus_tag="VLE22_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSB15687.1"
                     /translation="MSAGKEEFYRIQKLPPYVFAVVNEMKARLRAKQYDVIDLGMGNP
                     DGVTPKAVVSKMIEAARNPRNHRYSISRGIPNLRLEIVKRYKSRYGVELDPESEAIVT
                     IGAKDALAHLLFAVIGSGDVVASPNPCYPIHQYGVVMAEGHACTVPMPDPGTFLGHLK
                     DLYKEARKPRMILISFPHNPTTQCVDLEFFREVVALAAAHGTMVVHDFAYADICFDGY
                     EAPSILQVEGAKEVAVEIYSMSKSFNMAGWRVGFCLGNRKMIAALARIKSYLDYGIFQ
                     PIQIASIIALRECEADTRKICAVYQRRRDVLIEGLNRAGWPVAPPKGSMFVWAEVPER
                     FRAMGSLEFSKLLLEKALVAVSPGVGFGPLGEGYVRFALVENDHRIRQAVRSVKSFLG
                     RE"
     gene            5770..>6761
                     /locus_tag="VLE22_14615"
     CDS             5770..>6761
                     /locus_tag="VLE22_14615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB15688.1"
                     /translation="MFTLRRRVRVVGRRLREARMFARAMHSPDHPIAAQIVPIRRCNL
                     ACTYCNEFDAISRPVPVAEMLRRIDLLAALGTTIITVSGGEPMLHPELDEIIRQIRHR
                     GALATLITNGYLLTPERIQRLNRAGLDYLQISIDNVVPDETSKKSLKVLDRKLELLAA
                     DAGFAVTVNSVLGSPVRNPGDALVIAERARSLGLTSTVGILHDGSGQLGPLAPDHASI
                     YERILAFEQSLFSFAQYDRFQRKIAHGLPNDWHCRAGGRFLYICEDGLVHYCSQQRGH
                     PGIPLEQYTHDHLKREAARPKPCAPFCTISCVHQTAMLDAFREDPHSTLAGMIER"
BASE COUNT         1307 a         1992 c         2191 g         1271 t
ORIGIN      
        1 atgagaaggg gacggaccga atcgtcattg gcccagttcc ggatcctcag ataatgggca
       61 agcgaatgaa acgccgcagt gcgggcaccg gagttcagct aaacgatgcc agcggcaacg
      121 aacgcggcgg actcgcagtc atagacgacg gttcgatggt tgtgggcatc gatgatgagg
      181 cgggccgaga acgtgctcac ttgtacttca taccgaagaa aggttctggc ttgctattac
      241 agggagaaag cggaaagcag aagatatccc tcgcggttcc cccggaaggg gaacaatcgg
      301 cgagcccgca actcgaaatg acggaccggg ccggaaatcg tgtggctgcc attccaaccc
      361 ggaagtgatt ctagttgcag tgggcgatca attcgatgaa tcgcctcaga ccgacttcgc
      421 tcaggtcggg ccggtggtat cgcggcggcg cactgggccc tgacgagggc cgcttcaact
      481 tcctcgccta gcaggctgcg gaaaaactcc cgcagcacgt cactgtgttc gttcgtcgtc
      541 gctcggaggc cgaacgtatg cgagtgcctc tctttcgctc gctccaaatt ccttgaagtt
      601 ctctcgtgcc tgagccaaag tgatgccatt tgccccatca ccggcgtgct gcggatcagc
      661 atattggacg gggtcatctt cccaaaaaca gacagggcag atttcgtagc tgccaggcgg
      721 ttcccttgtg agagtgagaa agccgcagca cggacacgga tatttcacat tctgcatttt
      781 gccacggttg tgccatctcg cggtcgcgag gcagcatcga cggccactgg ccactccctt
      841 ctcaaatacc tgccagtaca agttaccgtg caaacgtttc gatcgtctat atctgtaatg
      901 cgaggcctcg atcatcagca gaccaatgtg ttcagctatt tgtcgccgga agagcgggta
      961 ggtaaggacc atcctttgcg ggcgatccgc accatggcgg atcgggctct gggggtctcc
     1021 gttgttcgat accatgtacg ccgcgacagg ccgcccgatg gcgggcacct gtacttcggg
     1081 agcgaccgca ccggcgactg gagcctgtgg cgtgtgccgg tggccaacgg caaggccggg
     1141 ggaccggcag aattggtgag gcagaacacc ggcaggctca ggccgatcgg cttcaccgca
     1201 gagggcacac tctactacag ccttgaaaac cgaggcagcg acatctactc cgcggatctg
     1261 ggcccggata cgtcaaaggc agccggggca ccgaagcccc tcatcgagag gttcatgggg
     1321 aagaacgcca accctgtctg ggctcctgac gggaagcgtc tcgcctatta ctcaatgcgg
     1381 gggcacccgc ccacgatggg cgtcctctcc atccgcaacg aagagacggg tgaagaggtg
     1441 gacctttacc cgaagctggc ttggttcaag ggtctgagct ggttcccgga aggacgctct
     1501 cttctggcct atggagtcga tctgacgaat acgatggcct acttccggat cgacgcggag
     1561 accggtgagg tcaacaagct cttcgatgtc gattttcgag gcctcgtccc ttgggccgtt
     1621 ctgtctcccg atgggaaacg gctatacaac ctgggccccc gggggcttgt cgagcacgat
     1681 ctggcaaacg gtggcgagcg ggcggttgcg cggccgccta aaggcgccga gttcgttgag
     1741 ctcgccatgt cgccggatgg caaattcctc gccaccaagc tcaacaagag cccttacagc
     1801 ctcgcaattc ttcctgtaga caccggtgaa gtccgagaag tgctgaccat tccgccgccg
     1861 aactggttcc tgagcatagc ctggacaccg gacagcaggt accttctgtc cgccaaacag
     1921 ttcgaggtag agaatgagag agctgaactc tggcgggtgg cggtcgcgac cggcgaggcg
     1981 caaagcctgg gactctcgtt ccagagcatc cgtgacctct cgatccaccc caacgggcgt
     2041 cgcatcgcct tcacgtacgc gcaccggaca agcgaaatct gggcgatgga gaacctcggc
     2101 accggctctg cgaggtagaa tccaggctcg aagaacaact ctccgtttcg tgccccggaa
     2161 gtggccacag gcccggtttt gacttcccaa agatacacaa aagttactcc agattgagtt
     2221 aggagataat gatgttggct ggcatgaacc aacgtccgat cgctgcattc gttttgcttg
     2281 tctgcaccgg cggcatcgcc gttgcccaga gtgggccaca cgtgcgtttc caagcgcacg
     2341 ccgcgacggg tgtcgaggag ccggaccggc gcatgacgta cgggctgaac attacgcctc
     2401 cgtgggagga cggaggattt ctgttcgtca accttcccga gcacttggaa tacatgcccg
     2461 gcacaaaggg catcgcccgg caccacgatc cgcggccgaa cgtctggcgc gtttcggaag
     2521 atggagaccg agccagttat gacgtcgaga gccagaccga acctggagtt ttcttgcggt
     2581 gtgaggctcg cgcagaggct cagcgcgccc ggttcgagtt gacgatcacg aaccgctccc
     2641 gaaagaccct aaagagtatc cggccactcc tgtgctacca ataccatcat ttgaagggct
     2701 ttccggcggc acggaccgat aatttcgcac acaccttcgt tgtgagcggc ggcaaaccag
     2761 tgcccgtatc agcactaccg gtgaaacgcc cggaggctta cgcacggatg gcacaggtcg
     2821 ctgggtgccc ggacgaacac aactggtggg ctgagcggat gggcgggttc atcgaaccac
     2881 gtctggacac ggccatcacg gtgcttaccg cttccgacga cgaccgaaag attgcggttc
     2941 ggtggcgacc cggaaaaaac ctcctgacaa acagtgcgat tccgtgtatt cacgccgacc
     3001 catgcatcgg cgatctgaaa ccaggtgagt cccggacggt gtcgggagac gtgattttta
     3061 tgcgggcacc gctcgaagat ctcatccgtt agccccgagt tactatccgt tttcagatga
     3121 agattcttgt gtttggctta ggcagcgcgg gagacgtgca ccctaacgcc ggcttggggc
     3181 tggctctgca ggagcgcggc caccgcgtgg tattggctgc ggcgagtgtc tttgagccac
     3241 tggctcggcg tctagggctg gatttcgccg gtgcgggcac ggaaggggag tacaacgacg
     3301 caatccagga tccggacctg tggcacccct ggcgttcctt caacgtggtg gcgaatcgcc
     3361 tgatgcttcc gtggatgcgt aaaacatacg agatgatcgc caatgcgtgg gagtctggca
     3421 gaatcgtggt ggcagctccg gcgaccgcgc tgggcgcgcg cattgcgcag gagaagctgg
     3481 gcgtgccact ggcgacggtg catttgcagc cgtcgatgct gcgcagcctt gtgcagccga
     3541 gctgctacgg gtttcccgac attatcggcg ccctgccgca ctggttgcga gagccctaca
     3601 tgcggctcgt cgaccgccag ataatcgacc ggccactggc acgaagtgtc aatgcttttc
     3661 gcgccgagct tgggttgccg cccgtgcggc gcctgttcaa cgagtggatc cattcccccg
     3721 agctggtgat cggaatgttc ccgccgtggt ttgctccacc gccgcctgac tggccgccga
     3781 acgtacacct gaccggcttt ccgctgtatg acgagaggga ggcgcggcag atacagccgg
     3841 agttggagga tttcctccgg atgggcgagc cccccatcgt gttcacggca ggctccgcga
     3901 tggcgcaggg caaggagttc ttcagcgtct cggcggaagt gtgccggatc cggggctgga
     3961 gaggtatcct gttgacacag tatccggagc aactcggcac tcttccggac agcgtacggc
     4021 atttcaatta cgtgccgttc agcaccgtgt tgcccagggc ggcggccttt gtccatcacg
     4081 gcggcattgg gacgacggcc caggccctgg cagccggagt tccgcaactg gtggtgccat
     4141 tcgcgcacga ccagccggat aatgcagtgc gggtgcgaag gctgggtacg ggcgactacg
     4201 tcagaccgaa ggactaccag gcgaatcggg ttgttcgcaa gctggaagcc ctgatgggat
     4261 ccacagacat ccgtgagaac tgccggctga gggcggaggc gctggccaac agcactgcgc
     4321 tgaacagcgc gtgcgaattg attgagcggc tggactagac gttaacacga gtgtagaaag
     4381 agacgctccg tgaaggctcc gctccctgac ggtcgcggtt caaaagcgct gccggacttc
     4441 cgctgcgtcg agcggcgaaa tggtggtcag tcccatttct tcttagaata gaggaagtgt
     4501 ccgcaggcaa ggaagaattc taccggatcc agaagttgcc gccgtacgtg ttcgcggtgg
     4561 tgaacgagat gaaggcgcgg ctgcgcgcca agcagtatga cgtgatcgac ctcgggatgg
     4621 gaaatcccga cggggtgaca cctaaagccg ttgtttccaa gatgatcgag gctgcgcgga
     4681 accctcggaa ccaccggtat tcgatatcgc gggggattcc gaacctgcgg ctcgagatcg
     4741 tgaagagata taagagccgg tacggcgtgg aactggaccc ggagtcggag gcaatcgtga
     4801 cgatcggggc gaaggacgcg ctggcgcacc tgctgttcgc cgtgatcggg tctggggacg
     4861 tggtggcgtc gccgaatccc tgctatccca ttcatcaata tggagttgtg atggcagagg
     4921 ggcacgcgtg cacggtgccg atgccggacc cggggacgtt tctggggcac ttgaaggacc
     4981 tttataaaga ggcgcggaag ccgcggatga tcctgatttc gttcccgcac aatccgacga
     5041 cgcagtgcgt ggacctggag tttttccggg aggtggtggc gctggcggcg gcgcacggga
     5101 caatggtggt ccacgatttc gcgtacgcgg acatctgctt cgatgggtac gaggcgccgt
     5161 cgattttgca ggtggagggg gcgaaagagg tcgcagtaga aatctactcg atgtcgaaga
     5221 gcttcaacat ggccggctgg cgggtggggt tctgcctggg gaaccggaag atgatcgcgg
     5281 cgctggcgcg gatcaagagc tacctcgact acgggatttt ccagccgatt cagattgcgt
     5341 cgattatcgc gctgcgggag tgcgaggcgg atacgcggaa gatttgcgcg gtgtatcagc
     5401 ggcggcggga cgtgctgatc gaggggctga accgggcggg gtggccggtg gcgccgccga
     5461 aggggtcgat gttcgtgtgg gcggaggtgc cggagcggtt ccgggcgatg ggctcgctcg
     5521 agttttcgaa gctgctgctg gagaaggcac tggtggcagt gtctccgggt gtaggattcg
     5581 ggccgctggg cgaggggtat gtgcggttcg cactggtgga aaacgatcac cggatccggc
     5641 aggcggtgcg gtcggtgaag agtttcctgg gccgcgaata agggtttctc gggaagcgcg
     5701 caaagggcgc caaaggtttc ctcgcacgat acaatgcggg taatggggcg tgcggctggg
     5761 aagtcgttgg tgttcacgct tcggcgccgc gtgcgcgtgg tcggcaggcg gctccgcgag
     5821 gcacggatgt tcgcccgcgc catgcattcg ccggatcatc cgatcgccgc ccagatcgtg
     5881 ccgatccgcc gctgtaacct ggcctgcacg tactgcaacg agttcgacgc catctcgcgg
     5941 cctgtgccgg tcgcggagat gctgcgtcgc atcgatttgc tcgccgctct cgggaccacc
     6001 atcatcaccg tcagcggcgg cgaaccgatg ctccaccccg agctggacga gatcatccgc
     6061 cagatacggc accgcggcgc cctcgcgacg ctcatcacca acggctatct cctgacgccc
     6121 gagcgcatcc agcggctgaa ccgggccggt ctcgattacc tgcaaatcag catcgacaac
     6181 gtcgtgccgg acgaaacctc gaagaagagc ctgaaggtcc tcgaccgcaa gctcgaacta
     6241 ctggccgcgg acgccggctt cgccgtgaca gtcaattccg tcctcggcag tcccgtccgt
     6301 aacccgggcg acgcgcttgt catcgccgag cgcgcgcggt cgttgggcct gaccagcacg
     6361 gtcggcatcc tgcacgacgg aagcggccag ctcggcccgc tggcccccga ccacgcctcc
     6421 atctacgagc gcatcctggc gttcgagcag tcgctgttct ccttcgcgca gtacgaccgt
     6481 ttccagcgca aaatcgcgca cgggctgccc aacgactggc attgccgcgc cggcgggcgc
     6541 ttcctctaca tctgcgagga cggccttgtc cattactgct cgcagcagcg gggccatccg
     6601 gggatcccgc tcgaacagta cacccacgat cacctgaaac gcgaggccgc acgcccgaag
     6661 ccgtgcgctc ccttctgcac tatttcctgc gtgcaccaga ccgcaatgct cgacgctttc
     6721 cgcgaggacc cgcacagcac gctcgccggc atgatcgagc g
//
LOCUS       DATIFQ010000312        19987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_869205, whole genome shotgun sequence.
ACCESSION   DATIFQ010000312 DATIFQ010000000
VERSION     DATIFQ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19987
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869205"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..2549)
                     /locus_tag="VLE22_14620"
     CDS             complement(411..2549)
                     /locus_tag="VLE22_14620"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HSB15689.1"
                     /translation="MSPKNLIRYLALAACSLGITEPVWSQPGHWPGEYKVEREDAAGT
                     LTLSTPYYTFRHDLKRGAEISLIAYTHGRSHNLLVTPVDAGVRMAGGDSFSAGNDSSP
                     EVSVEKSGKAQVVLVNCRLLDAKGRDSGLRLRTRYEYRWGYVKIRREFVFAGPTEVGM
                     VNVLSATFDPSLEQYGYRQGMAEQEGVSPSSFGVMQWRKLRAGAFFDPPLQTRIVPRY
                     LVLANRGIEGIEWFVSDDLGQWDYQAAGKPGAGLCAVRSSLAPLGVAVNIRPLDVQGH
                     VSAQGTWTFDSYLGVPILEGHANAPWLHASFARNGGEWVPEAKVKQWADSGIRTAHCH
                     NDGDSRGDGLFWRDGSYPPYPPEDMKKYDMTIDLCHKYGIRVGTYFSNKELHPTTEDY
                     KQHAEEWARKPDDTGRLYHNPTRYGEYGAQMCLKSSWLKTLERNIDTVLKNHKLDGVY
                     YDWNVALYCNNPLHVGKTSNGVSGHTGTLALSPTGHWDIDELIQLMEWTRERVGPKGL
                     IFVHNTMAPMFATENFADRVVGMEWGYGLLSRSVPPIQELPLEWEFAGARSRAVIEYG
                     SIDRSAPARLRKAFALEALFAGVTPWPASPETVDLFKVLTPLGDLTGYKFEDWRNRAV
                     KLDAPECISAVYSREGESWILLGNPTPEPRQVRCRVSPDGLPVPLRSVGSVRLMDRGR
                     STGLDAASLLGAGESVSIPADGAVLLRMTP"
     gene            3636..4070
                     /locus_tag="VLE22_14625"
     CDS             3636..4070
                     /locus_tag="VLE22_14625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB15690.1"
                     /translation="MPVKPIPEGYHTVTPYLVVPGAGRLIEFLKEAFKAEEVERMSQP
                     DGTVMHAEVRIGDSIIMMGESSDKFPAMPAAIYVYVPDVDAVYKRALAAGAASTMEPA
                     NQFYGDRNAGVKDPSGNLWWIATHVEDVPREEMAKRAAAAKK"
     gene            4160..5335
                     /locus_tag="VLE22_14630"
     CDS             4160..5335
                     /locus_tag="VLE22_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB15691.1"
                     /translation="MTDNQRNSSASRPEHCTITRRSFVGESVALAGGLCAVASTAAVR
                     TETPRGKILNHNSQMEYRRLGKTGLMVSAVCLGGHWKRVAVALNRPFTGSGASKEDFD
                     NIKASDFLKNRHDVISRCIDAGINYVDACSPMEILAYAKVLKGRRDKFYFGYSWHLRE
                     PRFLEWRTSKKLIEGLDAGLKEAGLEYVDVWRISLPQERVADLGELLRVEEATVEGLE
                     KAKQQGKARFTGVSTHNRVWLKSVIEEYPKQMEVVLFPYTADSKQLPDDSVFEAIQQH
                     DVGVFGIKPFADNALFRGDGSPNSPHAEEDSRRARLALRYILSNPAITAPIPGLVTLA
                     QVDNVVKAIAERRAHDLSAPAKDTRRISESIERQAAAQEMWANLSPDHRWLKNWEYV"
     gene            5345..6631
                     /locus_tag="VLE22_14635"
     CDS             5345..6631
                     /locus_tag="VLE22_14635"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15692.1"
                     /translation="MSIITRRSFFQGAALAVSATRVVGANDRIQIGIVGLGGRGNAHL
                     RNFIKIPESQVVALCDVNQAARERAQARLMNETSAKGKEYADMRDMFADKSVDAVSTA
                     TPNHWHALTTIWACEAGKDVYVEKPASHNIYEGLRMVEVARRTKRMVQVGTQSRSTPH
                     KIRAMQLLRDGTIGKVYMAKGLCFKRRKSIGKTPVEPVPPGLDWDKFLGPAPMRPYTK
                     NRFAYNWHWFWDTGNGDIGNQGVHEMDLCRWGAGEAGMPRSAVSKGGKYVYDDDQETP
                     NTQLATFDYGDMEIVFEVRGLLTGPEAGLPVRPGNTVGNIYYGSDGWMWVGGGEFAVY
                     KGESNEKVMEEKSASEDGSVLHMKNFLDACRSRNYKDLTANIEVGAMSAALCHLANVS
                     YRVGRTLRWNEAARKFVNDPEADKLISREYRKPYVV"
     gene            complement(6700..7581)
                     /locus_tag="VLE22_14640"
     CDS             complement(6700..7581)
                     /locus_tag="VLE22_14640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSB15693.1"
                     /translation="MPQTQPTAASRLQLLVAAFLFSTGGAAIKATSFSGWQVAAFRSL
                     VAAIALFLLVPAARKSWSRRTLLVSLGYAGTLVLFVLANKLTTSANAIFLQDTAPLYM
                     LLLGPWLLREPVRRSDFLLMVVIAAGMSLFFVGGQDVQRTAPDPLRGNIIAAFAGVCW
                     ALTIGGLRWIETASSGRESGMATVVAGNLIACLICLPKALPLSAPSMADWAVILYLGV
                     FQIGLAYVFVTRAMRRTPALEASLLLLAEPAMNPVWAWLVHDERPGGLAIVGGALILA
                     ATIGRTLARTGNFADSR"
     gene            7657..10833
                     /locus_tag="VLE22_14645"
     CDS             7657..10833
                     /locus_tag="VLE22_14645"
                     /inference="COORDINATES: protein motif:HMM:NF015617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HSB15694.1"
                     /translation="MGASIPVNLPRPEHGLNYKTPATVWDEALPLGNGMIGALVWGDG
                     KPLKISLDRADLWDLRLVPEFYTEEYKFRIMRQWHLAGRVKDQERVYEEPYRRPAPTK
                     IPAGRIELALGGAGKFRETALSLGDALASFALDDGTTARVMVHAVQPVGIITVQGPAV
                     VSPRLVAPAFAGKVVEAARGGIGAGDLTQLGYPAPKESSGQDFQAFLQQGAEGFRFAV
                     YLGWRTTGRGWTGAWSVASSFEGGDPLSLAKQRVEQALTSGFEPMMKTHRQWWESFWK
                     KGTVLLPNPIVERQWFLETYKFGAAARRGAPPITLQAVWTADDGKLPPWKGDYHHDLN
                     TELSYWPCYSGNRIEEGLSYLDYLWNTRKAGFDWTKRFFELPGVNVPMTADLNGNQIG
                     GWRQYTHSATTAAWLAQHFYLHWKYSADREFLKERAYPYLHDSAIFLEAFTAEKGPDG
                     KRTFPLSASPEINDNRPTAWFPTVTNYDLALTRFLFAATAELAEELGRWDDVKRWRKV
                     LGELPEYSLGDDGRLLVAKDNPLRQSHRHFSHLMAIHPLGLIDPMDGNEAVRTIRASL
                     SELMQLGSDWWCGYSFSWLANLAARARDGEEAEKALELFSTAFTLRNSFHCNGDQTGK
                     GYSKFTYRPFTLEGNFAAGAGIQEMLLQSQRDKIQIFPAVPASWKDVSFTTLRAQGAF
                     LVSAERKAGKTTRVEITSEKGGEAVLISPFSGKEMRLRMKPGETQVLLADPPAAAARQ
                     SAVERVLNGWFRWSSSEPLVAPAVGDGDQYHSVKDPTIVRHNGRWHIFCTVRGKKRSH
                     QIEYISFTNWRDTGKASRRMLKLTDGYFCAPQVFYFRPHKKWYLIYQVIDKSRKPALQ
                     PAWSASADIANQDTWTAPALLFDKEPEGVKSWIDFWIICDDRRAHLFYTGMDGTMWRA
                     ETELENFPRGWGKPVLALEGDIFEASHTYRLKDLQKFLTVVEAIGEHGRRYYKAYLAD
                     RLDGAWQPIAGTSFRPFASPANVRFAGARWSDAFSHGELIRDGYDETLTVDPAALHFL
                     YQGVTDADREGKPYGEIPWQLGLLTPME"
     gene            complement(10817..11911)
                     /locus_tag="VLE22_14650"
     CDS             complement(10817..11911)
                     /locus_tag="VLE22_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB15695.1"
                     /translation="MTPRNELSPRERVLLALEHRDTDRVPVDFLATPETWAALKTHLV
                     INDPESVLRRLGVDVRHPRQPYVGPPLRCFPDGTWLDPWGVRRRAVAHEKGSYDEITE
                     HPLAAVNDASELQRYPWPRPEWWDAGGLSAQIRELDRQGDYAIALEEFGDPGGIFEIA
                     WYLRGMERFMLDMVEQPGLVWEIMRRVTDFYVGMLELVMAAAGDRIDLIWTSDDIAHQ
                     RGRLMSERAWKQLVAPHHERLNRRVHELGSLVMYHSCGAVRPFIPALIGIGVDVLDVL
                     QFSAAGMDPRDIKETFGDRLCFHGGMDVQTTLPQSSAEEVRRVTRERIDVLGRGGGYI
                     LSPTHNIQIDTPPANIVAMYEEAGSFTPSE"
     gene            11988..12557
                     /locus_tag="VLE22_14655"
     CDS             11988..12557
                     /locus_tag="VLE22_14655"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HSB15696.1"
                     /translation="MTKSNIRYTRYFAETIQRMREDGLLLVTAHNDGKPNVMTIGWGT
                     IGSIWGRPIFLALVRPSRHSHSLIEEVSEFTVNVPPRELASAVSHCGTVSGRDHDKFD
                     EAKLKVKPSRMVKPPIVEECVIHYECRVVHKNDLVPESLTQAIRDDFYEQGDFHRVYF
                     GEIVAAYADEDADIRLRPKQAAGPLPLAG"
     gene            complement(12540..14960)
                     /locus_tag="VLE22_14660"
     CDS             complement(12540..14960)
                     /locus_tag="VLE22_14660"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15697.1"
                     /translation="MRRLIACAAILVSFTVFDAAGGETSGFLSAKPVWPAGRETEMNL
                     FVGFRVAFEAPAGARAVFRVTASSLYRAWLNGEFLGHGPARGPHGFFRIDEWDLSGRL
                     RPSGNLVAIEVAGYNANSYYLLDQPSFFQAEVVSGGKVIASTAGEGAPMAATILTERL
                     QRVERYSFQRTFSEVYRLRPSWDAWRRDSAASLVPAETAVLPARRLLPRRVPYPDFVQ
                     RRPLALLAEGTVQRGVAPGKLWKSRGLVEIGDRLKGFREAELEQMPSRELQQLAKSMS
                     RRLDQELEGPAAFTFPVNSFRILDFGTNLTGFPGATITVNAPAKVYFVFDEILTGDDV
                     DFKRLSAVNVIAFDLEPGSYRVEAIEPYTLRYLKILVTGAGGKVADIYLREYVNPDVD
                     RAHFASADPRLNRLFSAGRETFRQNAVDIFMDCPHRERAGWLCDSYFTARVEPRLSGR
                     NVIEKNFFENYLLPESFPVLPRCMLPMCYPADHTNGTFIPNWSLWFVLELEEYLARSG
                     DRETVDALRPKVLCLFDYFKKFRNPDGLLEKLPSWVFIEWSAANNFVQDVNYPSNMLY
                     AAALDAAARLYSMPEMAAAARSVRAAVIRQSFDGEFFVDNAVRTSGRLERTNNRTEVC
                     QYFAFYFNVVSAHSYPELWETLRDKFGPDRPKSGAYPEIHAANSFVGNMLRLELLSRY
                     GLSQQLLDESVSYLLYMAERTGTLWENVDPSASCNHGFASHIVHTLYRDVLGLHSVDV
                     VNKVVKLRFADLNLPWAEGEVPVSGGRVSLRWHKTGDGGLAYRVDVPAGYKVEVEKLG
                     SRPLSRER"
     gene            15001..16221
                     /locus_tag="VLE22_14665"
     CDS             15001..16221
                     /locus_tag="VLE22_14665"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSB15698.1"
                     /translation="MRPQPILVLLWFAISCSAQFTNPGWLHLSSARGEIDAPNAGGQQ
                     TSATVFDIDKDGVNDFVITERTAAPSVIGYLRTKSGWRRFVIEPELVKLEAGSTFGDV
                     DGDGDLDFLSGNDYSGNGVWWWANPHPNFAPDAPWTRHPIKTSGPRKHHDLMFADVDG
                     DGKNELVFWNQGGNRLVLARVPASPRAYAGEWPMAEIFTYASDSEQQQRAASPPFKGI
                     NEHEGLALADIDGDGRPDIVGGALWFKHLGGDRFRANEIDGGYHFSRSGAGQLKEGGR
                     PEVVLVVGDGVGPLVMYEWVKGTWKPNVLIDEIDNGHSLAVTDFDRDGHLDIFCAEMR
                     LNGGNSDSKVYILLGDGKGGFRQTVVATGFDNHESKLADLDGDGAPDILGKPYNHGAP
                     RLDIWLNRLAVTRQ"
     gene            complement(16215..17216)
                     /locus_tag="VLE22_14670"
     CDS             complement(16215..17216)
                     /locus_tag="VLE22_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB15699.1"
                     /translation="MTSRERVLAMLDGRPVDHLPLMPITMMFAADRIGEKYGRYALDH
                     RIMAEAQIRTAEEFGFDHVSGITETREAPDCGASVQYFDDQPYAMDESRARLAEKSAL
                     KDLKMPEPCGGAMNDRLLGLLLMKERAGSEKIVEGWVEGPCGASADLRGINTLMLDFF
                     DDRAFVTNLFEFVVELAVRFGRAQVEAGADVIGIGDPASSLVGPRIYEEFVWPYQKRL
                     ADGLHAAGARVRLHVCGNTRRMLTGMAKLGCDIVDIDSQVPLAEAREKMGPNQVLLGN
                     LDPVRVLRDATPEAVFAALMECHRQAGPRYIVAAGCEVVRDTPLANMRALAEFARSH"
     gene            complement(17235..18317)
                     /locus_tag="VLE22_14675"
     CDS             complement(17235..18317)
                     /locus_tag="VLE22_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15700.1"
                     /translation="MRFLLALVLAAAPAAAQHRDALPVPDLPGYRTLKCDFHTHTVFS
                     DGEVWPTTRVVEAWRDGLDAIAITDHAGYNPHEKDLKPDLSRPHAIALPLAEQLGLIL
                     VPAVEIAEKDFHCNALFVTDFNAFFSLGMAEALEVARKQDAFVFWNHPGWKETPQWEP
                     PASTLHERGLIQGFELVNETTVYPEAFPWAKEHRLAIFANSDIHAPATAFYTSRERPV
                     TLVFVRTPDLAGIRDALPARRTAAWVNDEVWGAEEHLTALWKGAVQVENPEVRLKPGA
                     RLALRLRNRSAIPFRIRMTGAPSWLRIGDAEVRPEGVVGTALSVSKGAPPGTHEVELD
                     TEVGNFHITPERNLMVKVLLRVEISK"
     gene            complement(18322..18852)
                     /locus_tag="VLE22_14680"
     CDS             complement(18322..18852)
                     /locus_tag="VLE22_14680"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HSB15701.1"
                     /translation="MRKTVTLLSFVAVGLTAGAVLLAQGQKRVSPHETVTEVFGGKKI
                     SIEYGRPYKKGREIFGGLVPFGKVWRTGADEATILTTEADLMVGSIHVPKGSYSLFTL
                     PDQSSWMLILNKVAKQWGAYKYEETQDLGRTKLEVSQGPEVEQFTISISKTNNGKGAI
                     TMKWDKTIAVAPIMVH"
     gene            18985..>19987
                     /locus_tag="VLE22_14685"
     CDS             18985..>19987
                     /locus_tag="VLE22_14685"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB15702.1"
                     /translation="MNLRTLSLLIAVILAYSQVAAQKTADEYAAQGLKLFENGRYAEA
                     RKDLEAAVKLDRTQVEALLLLVRIHLIEKDYDGAVELVAPLAQSPAADAESRMLLAYT
                     LEKRGDLRRAAGLLERSIEIITWGGDATYAAAARARMKLGDYERAAALCERGMESSPN
                     SERLLHTYVSLPADIIGKRVADRLARVPDEKGGVTERILLGRILSKIDVKSFPDAREN
                     SMKLLTEAATREPENCSAQYNLGRLERLVGRTKEAKGALEKALSLNPDDDLRTLIQTQ
                     LALAELASGDDAAAEQAYRSALDSNRRSLQPSADAALEYVRFLKLRSRDDEAKKLLNE
                     "
BASE COUNT         4058 a         5962 c         6294 g         3673 t
ORIGIN      
        1 gtagacggcg attatgttac tgtgtagtaa gccatgcagt tagtgagcga gaccgagact
       61 aacttacccc tgacgcttcc caagctggac gtcgcgggtt cgagtcccgt ctcccgctcc
      121 atttttcaac agcttaggaa ccatcagttt ttcccgttac cgcgtttact gcaattccca
      181 agaaacagca cgctgatcgg cggcaacctt gtcgtcaacg cccgccttcc gccccaggct
      241 ggcgtttgcc cgttcgattg ggggtagaac ggcgaggttc ggacgtgcgt catccccgag
      301 atccgaatga actccttgaa gtccttggcc acggcgggct tggacgccgc catccgccac
      361 agacctgcac tggaatcaag caggaatcgg cagatatcgc tattcaactc tcagggggtc
      421 atccgcaaca atacggctcc atcagcagga atgctgacgg actcgcccgc acccaaaagg
      481 ctggccgcat cgaggccggt cgagcggcca cgatccatca gccgaactga accaactgat
      541 cggagcggta ccggcagccc gtccggactg actcggcagc ggacttgtct cggctcggga
      601 gtcggattcc ccagcaggat ccatgactcg ccctcgcggc tatagaccgc ggagatgcat
      661 tctggcgcgt ccagcttcac tgcccggttg cgccaatcct caaatttgta tccggtaaga
      721 tcgccgaggg gggtcaaaac cttgaagaga tcgacggttt ccggactggc cggccagggc
      781 gtcaccccgg caaagagagc ttccagcgcg aaggctttcc gcagccgcgc cggagcactc
      841 cggtcgatac tgccatattc gatcactgcc cgagagcgcg cgccggcgaa ttcccattcc
      901 agcggcagtt cctgtatcgg cggcaccgaa cgcgaaagca acccatagcc ccattccatc
      961 ccgaccacgc gatcggcgaa gttctcggtc gcgaacatgg gcgccatggt gttgtgcaca
     1021 aaaatgagcc ccttcggccc gacccgctcc cgagtccatt ccataagctg gatcaattcg
     1081 tcgatgtccc aatgcccggt gggagaaagg gcgagcgtcc cggtatgtcc ggatacgcca
     1141 tttgaggttt ttccgacgtg cagcgggttg ttacagtaca gtgccacgtt ccagtcatag
     1201 tacacgccgt ccaacttgtg gttcttgagc acggtgtcaa tgttccgctc taacgttttc
     1261 agccagctgg atttcagaca catctgcgcg ccgtattcgc cgtagcgggt agggttgtgg
     1321 tatagcctgc ctgtatcgtc cggcttgcgc gcccactcct cagcgtgttg cttgtagtct
     1381 tccgtcgtcg gatgaagttc cttgttggag aagtaagtgc ctactcggat tccgtactta
     1441 tggcacaaat cgatggtcat gtcgtacttc ttcatgtcct ccggcggata aggcgggtaa
     1501 gatccgtcgc gccagaacaa cccgtcaccc ctagaatcgc catcattgtg gcaatgtgcc
     1561 gtgcgaatgc cggaatcggc ccactgcttt accttcgcct cgggcaccca ttcgcccccg
     1621 ttacgagcga aggaggcgtg gagccaaggc gcattcgcgt gcccctccag gatgggcacg
     1681 ccgagataac tatcaaaggt ccaggtcccc tgcgcggaga cgtggccctg aacgtccaga
     1741 ggacggatgt ttacagcgac accgaggggg gctaagctcg agcgtaccgc gcacaaaccg
     1801 gcgcccggtt ttcccgcagc ctggtagtcc cactggccga ggtcatccga aacgaaccac
     1861 tcaatacctt caatgccgcg attggcaagc accagatagc gcggcacgat tcgggtttgc
     1921 aagggcgggt caaagaaggc cccggcccgc agctttcgcc actgcataac gccgaatgag
     1981 cttggcgaca caccttcttg ctccgccatg ccttggcgat atccgtactg ctccagactg
     2041 gggtcgaatg tggcggacag aacattcacc atcccaactt ccgtgggtcc ggcgaaaaca
     2101 aattcccgac gtattttgac gtacccccac cggtactcat aacgggtccg cagccggaga
     2161 cctgaatcgc gtcctttagc gtccagcagc cggcagttta cgaggacgac ttgagccttg
     2221 ccgctcttct cgacggacac ttccggcgag ctatcgttcc cggcgctgaa ggagtctccg
     2281 cccgccatcc ggactcccgc atcgacgggc gtcactagca ggttgtgcga tcgcccatgg
     2341 gtgtaagcaa tcagggagat ctctgcgccc cgcttcaagt catgccggaa tgtgtaatag
     2401 ggagtggaaa gggtcagcgt tccggctgca tcctcgcgct cgaccttata ctcccccggc
     2461 cagtgcccgg gctggctcca gacaggttcg gtgatgccaa ggctgcacgc tgccagtgca
     2521 aggtatcgaa tcaggttctt cggtgacata atcatctctc cttcttcctt cgacaaacac
     2581 gtcgcggtcg cctacccatt tcagcctctc cttggataac cagacctgca cgcccaagtt
     2641 gtcgaatata ctcaacctct tatggcttcc tggccactgc cgattataca ttcgaccacc
     2701 tccgttggag ccgtggcttt catcattgtc gtcctgcgtg attccggcga ggcgagggaa
     2761 gtggtcagcc tcgcctcctc cggctacgaa aaggccggaa gtggactgca ccccgaaaat
     2821 gagataggaa gttaaggggc cgaacacatt gaatggagag gtgtatggat ttggcccgaa
     2881 gagtgtacag ccgggatctg aagattgcgc aagttgaggg tcccttcgag tggttggagt
     2941 gttcggactg aatcatagcc cacggcggtg gcgtcgaatg aacgtcgcgc cgaacgctcg
     3001 cggtggtcag tcagatcccg cacgaaatgg atggttctag gaagaacccc aaggtgcacc
     3061 ggacccaacg aggtccagcc agagtgcgga tgggagaccg gcggcaggat gggagaggat
     3121 caaggacgct cggggactca ccaccgtagt ggcggcagtg tcggagaaag ggagcgcgat
     3181 catccgcttc gatcccctgg cagggaccta cgatgctacg ctgacggtcc ccacgatgtt
     3241 atcccgcatg gggaattttt cggagatgtt gtccatgggg gattattcgg actcatccgt
     3301 tgtgtacgac ctgatcgaaa tgacgggcgg cgcttcatgt gcgccattgc ccaacaacac
     3361 ggtaccggcc tccagagaac agtctcctgt cgaggttcgg cggtttcctg cagattccct
     3421 gcggcaactc gctcccgaca cgcgacgaca cgctccggct gttcgcagac ggcaaactgg
     3481 ccgcgtcgaa gagtgtcagc tacgccttgt cgaagcgata agggcgggtc caagcatggt
     3541 tcccttctca cggcccgcag tccctccggt gctatcctga tcgcagagtt gcaggcgccc
     3601 tacggggcgc gaaagccaca gagggggaaa gaaccatgcc tgtcaaaccg attcctgagg
     3661 ggtatcacac cgtgacgccg tatctagtgg ttccgggcgc cggccgattg atcgagttcc
     3721 tgaaggaagc cttcaaggcg gaggaggtcg aacgcatgtc tcagccggac ggcacggtga
     3781 tgcacgcaga ggtccgaatc ggcgattcca tcatcatgat gggggagtcg agcgacaagt
     3841 ttcctgccat gccggctgcg atctatgttt acgttcccga cgtcgatgcc gtctacaaac
     3901 gggctctcgc cgcgggggcg gcctccacga tggaaccggc gaatcagttc tatggcgatc
     3961 gcaatgcggg cgtgaaagac ccgagcggca atctctggtg gattgccacg cacgtcgagg
     4021 acgtaccgcg cgaggagatg gccaagcggg ccgcggctgc gaagaaatag cgcgaagagg
     4081 agcgagttca aaactggatc tgtcttgacc gccgtgcggc gagggggata cactctcagt
     4141 ccaaggcgga ggccggatca tgacggacaa ccagcggaac tcgagtgcat cgcgcccgga
     4201 gcattgtacg attacgcggc gctcttttgt cggcgagagc gtcgctctcg cgggaggctt
     4261 gtgcgccgtc gcgagcaccg ctgccgtgcg gacggagacg ccgcgcggca agatcctcaa
     4321 ccataactct cagatggagt accggcggct cggcaaaacc gggttaatgg tctcggcagt
     4381 ctgtctcggc ggtcactgga agcgggtggc ggtggcgctg aaccggccct tcaccggcag
     4441 cggcgcgagc aaagaggatt tcgacaacat caaggcctcc gacttcctca agaaccgcca
     4501 tgacgtgatc agccgctgca tagacgcagg gatcaactac gtggacgcct gttccccgat
     4561 ggagatcctt gcatacgcca aggtgttgaa gggtcgtcga gacaagttct acttcggcta
     4621 ttcgtggcac ctgcgggagc ctcgtttcct cgagtggcgc acctccaaga agctgatcga
     4681 gggcctcgac gccggcctga aggaagcggg actcgaatac gtcgacgtgt ggaggatctc
     4741 gctcccccaa gagcgagtcg cggacctcgg cgagttgttg cgcgtggaag aggcgacagt
     4801 cgagggcctg gagaaggcga agcagcaggg gaaagcgcgt tttaccggcg tctcaactca
     4861 caaccgggtc tggttgaagt ctgtcatcga agagtacccg aagcagatgg aggtggtgtt
     4921 gttcccctac accgccgact cgaagcagtt gccggacgac agcgtgttcg aagcgattca
     4981 acagcacgac gttggcgtgt tcggtatcaa gccgtttgcg gacaacgcac tgttccgggg
     5041 cgacggctcg ccgaacagcc cacacgccga ggaggacagc cggcgggcgc ggctcgcgct
     5101 gcgctacatc ctctcgaacc cggcgatcac cgcgccgatc ccgggccttg tcacccttgc
     5161 gcaagtcgac aacgtcgtga aggcgattgc cgagcgccgg gcgcacgatc tgagcgcacc
     5221 ggcgaaggac acccgcagga tcagtgaaag tatcgagcgc caagcggcgg cgcaggagat
     5281 gtgggcgaat ctctcaccgg atcaccgctg gctgaagaac tgggagtacg tttagggatt
     5341 cccgatgagc ataatcacac gccgttcctt cttccagggc gccgcgcttg ccgtttcggc
     5401 cacgcgcgta gtcggcgcca atgacaggat ccagatcggg attgtaggcc ttgggggccg
     5461 gggcaacgcc cacttacgca acttcatcaa gatcccggaa tcgcaagttg tcgcgctgtg
     5521 cgacgtcaac caggccgccc gtgaacgagc gcaggcgcgg ttgatgaacg agacgtccgc
     5581 gaaaggcaag gaatacgcgg atatgcgtga catgttcgcc gacaagagcg tggacgccgt
     5641 ttcgaccgca actccgaacc actggcacgc tttgacgacg atctgggctt gtgaggcggg
     5701 caaggacgtt tacgtcgaga aacccgccag ccacaacatt tacgagggct tgcgcatggt
     5761 cgaggtggcg cggcggacca agcgcatggt ccaggttggc acccagagcc ggagcacacc
     5821 acataagatc cgggcgatgc aacttcttcg cgacggcacg atcggcaagg tctacatggc
     5881 caagggcctc tgtttcaaac gccggaagtc gatcggcaaa acgccggtgg agcctgtgcc
     5941 gcccggcctc gactgggaca agtttctcgg cccggccccg atgaggccct atacgaaaaa
     6001 tcgattcgct tacaactggc actggttttg ggacacgggg aacggcgata tcggcaacca
     6061 gggcgttcac gagatggacc tgtgcaggtg gggagcgggc gaggcaggaa tgccgcgctc
     6121 cgctgtttcg aagggcggga agtacgtcta cgatgacgac caggaaacgc cgaacacaca
     6181 gcttgccacg ttcgattacg gcgacatgga gatcgtcttc gaagtgcggg gactgttgac
     6241 cggtccggaa gcggggctgc cggttcgccc tggcaacacg gtcggcaaca tctactacgg
     6301 aagcgatggc tggatgtggg tcggaggcgg cgaattcgcg gtgtacaagg gcgagtcgaa
     6361 cgagaaggtg atggaggaga agtcggcgag cgaagatggc tcggtcctcc acatgaagaa
     6421 cttcctcgac gcctgccgga gccgcaacta caaggacctc accgccaaca ttgaggtggg
     6481 cgcgatgtcg gcggcgttgt gccatctcgc aaacgtgagc taccgagtcg gccgcacgct
     6541 ccggtggaac gaggcggcgc ggaagttcgt gaacgatccc gaggcggaca agctgatcag
     6601 ccgcgagtac cgcaagccgt acgtggtttg aaggaggttg gggacaggta gctctgtccg
     6661 ctcgcttcgc tcaagacaga gtaggcctgc cccttcgaat taacgcgagt cagcgaaatt
     6721 gccggtgcgg gcgagggtgc ggccgatcgt ggcagcgagg atgagcgcgc cgccgacaat
     6781 cgcgaggcct cctggccgtt cgtcatgcac aagccacgcc cagaccgggt tcatcgcggg
     6841 ttcggccaac aacagcaacg aagcttcgag cgcgggagtt cggcgcatag ccctcgtcac
     6901 gaagacgtag gcgagtccga tttggaagac gccgaggtaa aggatcacgg cccagtcagc
     6961 catgctcgga gcgctcagcg gaagcgcttt cgggaggcat atcaggcacg cgatcaggtt
     7021 gccggccact acagtggcca ttcccgattc gcgcccagaa ctcgcggttt cgatccacct
     7081 caagccgccg attgtgagcg cccagcagac gccggcaaac gcggcgatga tgttcccgcg
     7141 aagcggatcg ggtgcagtgc gttggacgtc ctgaccgccg acgaagaaca ggctcatacc
     7201 ggcggcgatc actaccatga gcaggaaatc ggaacgccgc accggctcgc gcaacagcca
     7261 aggacctaga agcagcatgt agagcggcgc ggtgtcctgg aggaaaatgg cgttcgccga
     7321 tgtggtcagc ttgttggcga gcacaaacag cacgagagta ccggcatagc cgagggacac
     7381 gagcagagtg cgccggctcc agctcttgcg cgccgccgga acgagcagga agagagctat
     7441 ggccgcgacc agcgagcgaa aggcggccac ctgccagccg ctgaagcttg tggctttgat
     7501 ggcggcgccg cccgtggaaa acaagaacgc cgccacgagc aattgaagac ggctcgccgc
     7561 cgtgggttga gtttggggca cggtatactt gagcctgtca tgatagcaag gcgggtaatc
     7621 ctattcgttt cactgctgtt tccttcgagt tttattgtgg gcgcgagcat tccggtcaac
     7681 ctgccccggc cggaacacgg attgaactac aagactccgg cgacggtctg ggacgaagcg
     7741 ttaccgcttg gcaacggcat gatcggcgct ctcgtctggg gcgatggcaa gccgctcaag
     7801 atctctctgg accgggcgga cctgtgggac ctccggctcg tgccggagtt ctacaccgag
     7861 gagtacaagt tccggatcat gcggcagtgg cacctcgccg gccgagtcaa ggaccaggaa
     7921 cgtgtctatg aagaaccgta ccgcagaccc gctccgacga agattcccgc ggggcggatc
     7981 gagcttgcac ttggcggcgc gggtaagttc cgcgagacgg cactttctct tggggacgca
     8041 ctcgcgagct tcgcccttga tgacggaacc accgcgaggg tgatggtgca cgctgtccaa
     8101 ccggtgggca tcatcacggt gcagggtccg gcggtcgtgt caccgcggct ggtcgcaccg
     8161 gctttcgcgg gaaaggttgt cgaggcggcg cgaggaggga tcggcgccgg ggacctgaca
     8221 caactcggat atccggcgcc gaaagaatcc tcgggccaag acttccaggc gttcctacaa
     8281 cagggcgcag aaggtttccg attcgcagtc tatcttggct ggagaacgac cggacgcggc
     8341 tggaccggcg cctggtcggt ggcgtcgagt tttgaaggcg gcgacccgct ctccctggcg
     8401 aagcagcggg tggaacaggc gctcacatcg ggcttcgagc caatgatgaa aacacaccgc
     8461 caatggtggg agagcttctg gaagaagggg acggttctcc tgccgaatcc aattgttgag
     8521 cggcagtggt tccttgaaac gtacaagttc ggcgcggcgg cacgccgggg tgctccgccg
     8581 atcacgttgc aggcggtgtg gaccgcggat gacggcaagc tcccaccgtg gaagggagac
     8641 tatcatcacg acctcaacac ggaacttagt tactggccct gttacagcgg taaccggatc
     8701 gaggagggac tcagttacct cgactatctg tggaacacga ggaaggccgg attcgactgg
     8761 acgaaacgat ttttcgaatt gcccggcgtg aacgtcccaa tgacggcgga cctcaacggc
     8821 aatcagatcg gcggctggcg gcagtacaca cactcagcca cgacggcggc atggctggcg
     8881 cagcacttct atctgcactg gaagtacagc gcggaccgcg agttcctcaa ggaacgcgcc
     8941 tatccttacc tccatgattc cgccatcttc ctggaggcat tcacagcgga gaaaggtcca
     9001 gacgggaaga ggaccttccc cctcagcgca tcgccggaga tcaacgataa ccggccgacc
     9061 gcctggtttc ccacggtcac aaactatgac ctggcgctga cccgattcct gtttgccgcc
     9121 accgcggaac tggctgagga actcggacgc tgggacgacg tcaagcgctg gcggaaagtt
     9181 ctcggcgagc tgccggaata tagcctcggc gacgacgggc ggctactggt ggcgaaggac
     9241 aaccctctgc ggcagtccca tcgccacttt tcgcacctca tggcgattca cccgctgggg
     9301 ttgatcgatc cgatggacgg gaacgaggcg gtccggacga tccgcgcatc gctcagcgag
     9361 ctcatgcagc tcgggagcga ctggtggtgc ggctacagct tctcgtggct ggcgaacctc
     9421 gctgcgcggg cgcgagacgg cgaggaggcc gaaaaggcgc tcgaactttt ctcgactgcg
     9481 ttcaccctac gcaacagctt tcattgcaac ggcgaccaaa ccggcaaagg ttactctaag
     9541 ttcacctacc ggccgttcac gctggagggg aactttgcgg cgggagcggg gattcaggag
     9601 atgctgcttc agagccagcg cgacaagatt cagattttcc cggcggttcc agcatcgtgg
     9661 aaggacgtct cttttaccac gctgcgggca cagggggctt tcctcgtttc ggctgagcgc
     9721 aaggcgggga agaccacacg cgtcgagatt acctctgaaa agggcggcga ggcggtcctg
     9781 atctctccgt tcagtggaaa ggaaatgcgt ctccggatga agccgggcga aacgcaggta
     9841 ctgctggcgg accccccggc cgccgcggcg cggcagtcgg ccgtcgaaag ggtgctgaac
     9901 ggctggttcc ggtggtcctc gagcgagccc ctggttgcac ccgcggttgg cgacggtgac
     9961 cagtaccatt cggtcaagga cccaacgatc gtccgccaca acgggcgctg gcacatcttc
    10021 tgcacggtgc gcgggaagaa gcggtcccac cagatcgaat acatctcgtt taccaactgg
    10081 cgcgacacgg gaaaggcgtc gcggcgaatg ttgaaactca ccgacggcta tttctgcgcg
    10141 ccgcaggtgt tctacttccg gccgcataag aagtggtacc tgatctatca ggtgatcgac
    10201 aagtcgcgga agccggcgtt gcagccggcg tggtcagcca gcgccgacat cgcgaaccag
    10261 gatacgtgga ccgcgcctgc actgctgttt gacaaagaac cggaaggcgt caagagctgg
    10321 atcgatttct ggattatctg cgacgaccgg cgcgcccacc tgttctacac cggcatggac
    10381 ggcacgatgt ggagagccga aacggagctt gaaaattttc cgcgcggctg gggcaagccg
    10441 gtgctcgcgc tggaaggcga tattttcgaa gcgtctcaca cgtaccggct gaaggacctc
    10501 cagaagtttc tcaccgtggt ggaagcaatc ggcgaacacg ggcgacgcta ctacaaagcg
    10561 tatctcgccg accggctgga cggcgcatgg caaccgatcg cggggaccag tttccgcccg
    10621 ttcgcgtctc cggcgaacgt ccgcttcgcc ggcgctcgtt ggagtgatgc cttcagccac
    10681 ggcgagctga ttcgtgacgg ctacgatgag acgctgaccg tcgatccggc agcgctgcac
    10741 ttcctttacc agggagtcac cgacgcagac cgcgagggaa agccgtacgg cgagatcccg
    10801 tggcagcttg ggctgctcac tccgatggag taaaactgcc cgcctcttcg tacatcgcga
    10861 cgatgttcgc cggcggtgtg tcgatctgga tgttgtgcgt cggagacagg atgtagccgc
    10921 cgccgcgccc gagcacgtcg atccgttctc gagtgacgcg gcggacctcc tccgcggaag
    10981 attgcggcag cgtcgtctgg acgtccattc ctccgtggaa gcagaggcga tcaccgaagg
    11041 tttccttgat atcgcgcggg tccatgcccg cagcgctgaa ttgcagtacg tcgagcacat
    11101 cgacgccaat cccgatgagc gccggaatga acgggcgcac cgcaccgcag gagtggtaca
    11161 tcacaagcga gcccagctcg tgaacacggc ggttcagcct ttcgtgatgc ggggcgacga
    11221 gctgtttcca cgcgcgctcg gacatgagcc ggccacgctg gtgtgcaatg tcgtcgctgg
    11281 tccagatgag atcgatgcgg tcgcccgcgg cagccatcac aagctccaac atcccgacat
    11341 agaaatcggt gacacggcgc atgatctccc agacgaggcc aggctgctcg accatgtcga
    11401 gcatgaagcg ttccattccg cgcaagtacc aggcgatttc aaaaatacct ccggggtcgc
    11461 cgaactcctc gagcgcaatg gcgtagtcgc cctgcctgtc gagctcgcgg atctgagcgg
    11521 agagccctcc ggcgtcccac cattccggtc tcggccaggg atagcgttgc agttccgatg
    11581 cgtcattgac ggctgccagc gggtgttcgg tgatctcgtc gtaggacccc ttttcgtgag
    11641 cgacagcgcg gcggcgcaca ccccacgggt caagccaggt tccgtcggga aagcatcgga
    11701 gcggaggtcc gacatacggc tggcgggggt gccgtacatc gacgcccagt cgccggagca
    11761 ccgattccgg atcgttgata acgaggtggg ttttcaaggc agcccaggtc tctggcgtcg
    11821 ccaggaaatc cacgggaaca cggtccgtgt ccctatgctc gagggcgagc aggacccgtt
    11881 ctctcgggct gagttcgttt cgaggggtca ttctgttgta gcttgcctga tcagtgtaac
    11941 gcgccagcgt cggcgggata gaataagggc cggcaggaac tcatatcatg accaaatcca
    12001 acatcagata cacgcgctat tttgcggaaa caattcagcg aatgcgggaa gacggactct
    12061 tgctcgtaac agcccacaac gacggaaagc caaacgtgat gacgatcgga tggggcacga
    12121 tcggatcaat ttggggccga ccgatctttc ttgctctcgt ccggccgtcc cggcactcgc
    12181 actcactgat cgaggaggta tcggagttta ccgtcaacgt acccccgaga gaattggcaa
    12241 gtgccgtcag ccattgcggc acggtctccg gcagagacca cgacaaattc gacgaggcga
    12301 agctgaaggt gaagccgtca cgaatggtga agcctccgat cgtcgaggag tgcgtaatcc
    12361 actatgaatg ccgggtcgtc cataagaacg accttgttcc cgagtcgctc acgcaggcca
    12421 tccgcgacga tttctatgaa cagggtgact ttcaccgcgt gtatttcggc gagattgtgg
    12481 ctgcctacgc cgacgaagac gcggacatac ggctgcggcc aaaacaggcc gccggcccac
    12541 taccgctcgc gggataacgg tcttgacccg agtttctcga cctcgacctt gtaaccggcg
    12601 ggcacgtcca cgcggtaagc gagaccgccg tctccggtct tgtgccagcg tagcgagacg
    12661 cggccgccgc ttaccggtac ttcgccctcg gcccagggaa ggttcaggtc ggcaaaacgc
    12721 agtttcacca ccttgttcac tacgtccacg gagtgcagtc cgaggacatc acgataaagg
    12781 gtgtggacga tgtgcgaggc gaagccatgg ttgcagctcg ccgaggggtc gacgttctcc
    12841 cacagcgtgc ctgtccgctc ggccatgtaa agcaagtagg agaccgactc gtcaagcaac
    12901 tgctgtgaca ggccataacg ggagagaagc tcgagtcgaa gcatgtttcc aacgaatgaa
    12961 ttggcagcgt ggatctcggg ataggcgccg gacttagggc ggtccggccc gaacttgtcg
    13021 cgcagcgtct cccacagctc cgggtagctg tgagcggaaa ccacgttgaa gtagaacgca
    13081 aagtactggc agacctcggt gcggttgttc gtgcgctcga gcctgccgct cgtgcgcacg
    13141 gcgttgtcga cgaagaactc gccgtcgaag gattgccgga tgacggccgc gcggacactg
    13201 cgcgcggccg cagccatctc cggcatggaa tacagtcggg cggccgcatc gagggccgcg
    13261 gcgtaaagca tgttgcttgg atagttgacg tcctgcacga aattgtttgc ggcggaccat
    13321 tcgatgaaca cccaacttgg cagcttctcg agcagaccgt cggggttccg gaatttcttg
    13381 aagtagtcga acaggcagag aaccttgggg cgaagagcgt caaccgtctc acggtcgccg
    13441 ctgcgggcaa ggtactcctc gagttccagc acgaaccata acgaccagtt cgggataaag
    13501 gtcccgttcg tgtggtcggc cgggtagcac atcggcagca tgcaccgcgg cagtacgggg
    13561 aagctctcgg gcaacaggta gttctcgaaa aagttcttct cgatgacgtt cctgccactg
    13621 aggcgcggct caacgcgcgc ggtgaagtag ctgtcgcaga gccagccagc tcgctctcgg
    13681 tgtgggcagt ccatgaagat atcgaccgcg ttctgccgga acgtctcgcg ccctgcggag
    13741 aacaatcggt ttagcctggg gtcggccgag gcgaagtggg cgcggtcgac gtcagggttc
    13801 acgtactcgc gcagatagat gtccgccacc ttgccgccgg cgccggtcac gaggatcttc
    13861 aagtagcgga gcgtatatgg ctcgatcgct tccacacgat agctgcccgg ctcgagatcg
    13921 aacgcgatta cgttcaccgc cgagagccgc ttgaagtcga cgtcatcgcc ggtcagaatt
    13981 tcgtcgaaca cgaagtacac tttggcaggg gcgttgacgg taattgtcgc gcctgggaaa
    14041 cctgtcagat tggttccgaa atcgaggatg cggaaagaat tcaccggaaa ggtgaaggca
    14101 gccgggccct cgagttcctg atccagccgg cggctcatgg atttggcgag ttgctggagt
    14161 tcccgagagg gcatttgctc cagttccgcc tcgcgaaacc cctttaaccg gtctccgatc
    14221 tcaacgaggc cgcgcgactt ccatagcttc ccgggagcca caccgcgctg gacggttccc
    14281 tcggcaagca gcgccagcgg acggcgctga acaaagtctg gatagggtac acgtcgagga
    14341 agcaaccgcc gggccggcag cacggcagtt tctgccggaa cgagcgatgc cgcagagtcc
    14401 cggcgccagg cgtcccagct cggccggagc cggtatacct cgctaaaggt tcgctggaag
    14461 gagtagcgct cgactctttg caggcgctcg gtcaggatcg ttgcagccat gggagcgccc
    14521 tccccagccg tcgaggcaat gacctttccg ccggagacga cttcagcctg aaaaaacgac
    14581 ggctgatcga gcaggtagta gctgttggcg ttgtagccgg cgacctcgat cgcgacgagg
    14641 ttgccgctgg ggcgaagtcg gccggagaga tcccactcgt cgattcgaaa gaacccgtgc
    14701 ggaccgcggg cgggaccgtg accaaggaac tcgccgttca gccaggcgcg gtagagactc
    14761 gatgcggtca cccgaaagac ggcccgtgct ccggcaggcg cctcaaacgc gacgcggaac
    14821 cctacaaaca ggttcatctc cgtctcgcgc cccgcgggcc acaccggctt ggctgaaagg
    14881 aagccggatg tttcgccgcc ggctgcgtca aagacggtaa aagaaacgag aattgctgca
    14941 caggctatga gtcgcctcat cgctcgcaga ttgtagcacc ggtatagtaa tctcgacgcc
    15001 atgaggcctc agccgattct tgttctttta tggttcgcga tttcttgctc cgcgcagttc
    15061 acaaacccgg gctggcttca cctttcatcc gcccgcgggg aaatcgacgc accgaacgcc
    15121 ggtgggcagc agacgtcggc gacagtgttc gacatcgaca aggacggcgt gaacgacttt
    15181 gtcattaccg agcggacggc cgcgccgtcg gtcatcgggt atctccggac gaagtccggc
    15241 tggcgccgat tcgtcatcga accggagttg gtgaagctgg aggcgggttc gacgtttgga
    15301 gatgtggacg gcgacggcga cctcgatttc ctctccggca acgactactc cggaaacggg
    15361 gtctggtggt gggcgaaccc acacccgaac ttcgcacctg acgctccgtg gacacgtcac
    15421 ccgatcaaga cctccggacc gaggaagcat cacgacctga tgttcgcgga cgtcgacggc
    15481 gacgggaaga acgagctggt gttttggaat cagggtggca acaggctcgt cctggctcgg
    15541 gttccagcga gcccgagggc ctacgccggc gagtggccga tggcggagat cttcacgtac
    15601 gcgagcgaca gcgaacagca acaacgagcg gcctcaccgc cattcaaggg gatcaacgag
    15661 catgaaggac tggcgctggc ggacatcgac ggtgacggac ggcccgacat cgtgggcggc
    15721 gctctctggt tcaagcatct tggtggagac cgcttccgtg ccaacgagat cgacggcggc
    15781 tatcacttct cgcgctcggg ggcgggacaa ctgaaggaag gcggacggcc ggaagtcgtt
    15841 ctggtggtcg gcgacggcgt cggtccgctc gtgatgtacg aatgggtcaa gggaacgtgg
    15901 aagccgaacg tgctgatcga cgagattgac aacggccaca gcctggccgt gacggacttc
    15961 gaccgtgacg ggcatctcga tatcttctgt gccgagatga gattaaacgg tggcaattcg
    16021 gattcgaagg tctacatcct gcttggcgac ggcaagggcg ggttcaggca gaccgtggtc
    16081 gcaaccggct tcgacaacca cgaatcgaag ctggcggacc tggatggaga cggcgcaccc
    16141 gacattcttg gcaagccata caaccacggg gctccccgcc tcgacatctg gctgaaccgg
    16201 ctcgcggtta cccgtcagtg actgcgggcg aattccgcga gtgcacgcat gttggcgagc
    16261 ggggtgtccc ggacaacctc gcagccggca gcgacgatat agcgcgggcc agcctgccgg
    16321 tgacattcca tgagcgccgc gaagaccgct tcgggcgttg cgtcccgcaa tacgcggacc
    16381 gggtcgagat tgccgagcaa cacctggttt gggcccatct tctcccgtgc ctccgccaag
    16441 ggaacctgcg agtcgatgtc gacgatgtcg cagccaagtt tcgccatccc ggtgagcatg
    16501 cgccgcgtgt ttccgcacac gtgcagccgc acgcgagctc cggcggcatg cagtccgtca
    16561 gcgagccgtt tctggtacgg ccagacgaat tcctcgtaga ttcgcgggcc caccagcgag
    16621 gatgctggat ctccgatacc gatgacgtcc gcccccgcct ccacttgcgc gcggccgaat
    16681 cggactgcga gctcgacaac gaactcgaat aggttggtga cgaaggcacg gtcgtcaaag
    16741 aagtcgagca tcagggtatt gattccgcgc agatcagcgc tcgcgccgca cggcccttcg
    16801 acccatcctt cgacgatctt ctcgctgccc gcgcgctctt tcatcaataa caagccgagc
    16861 agccggtcat tcatcgcgcc gccgcagggt tcgggcatct tgagatcctt gagggcgctt
    16921 ttctccgcaa gacgggcgcg gctttcatcc atcgcgtagg gctgatcgtc gaagtattgg
    16981 acgctggcgc cgcagtcggg agcctcacga gtctcggtaa tgcccgacac gtggtcaaat
    17041 ccgaactcct cggccgtccg gatctgcgcc tcggccatga ttcggtggtc gagggcgtaa
    17101 cggccgtact tctcgccgat tcggtcggcc gcgaacatca tggtgatggg catcaatgga
    17161 aggtgatcga ctggacgtcc gtcgagcatc gcgaggacac gttcgcggct ggtcatcgtg
    17221 agtttaccta tgcgctattt cgagatttcc acccgtagca ggaccttcac catcaagttg
    17281 cgctcgggtg tgatgtggaa gttgcccacc tcggtgtcga gttcaacctc gtgcgtcccc
    17341 ggcggagcgc cctttgaaac actgagggca gtgccgacaa caccttccgg ccgaacctcg
    17401 gcgtcgccga tccgcagcca tgatggtgca ccggtcatcc ggatgcggaa cgggatagcg
    17461 gaacggttcc ggaggcgcaa agcaagacgg gcacccggct tcagccgcac ttccggattc
    17521 tccacttgga ccgcgccctt ccacaacgcc gtcaggtgct cttctgcgcc ccagacttcg
    17581 tcattcaccc atgccgcggt ccgccttgcc ggtagtgcgt cccgaattcc ggcaaggtcc
    17641 ggcgttcgga caaaaacgag agtcaccggc cgctctcgcg acgtgtagaa cgcggtcgcc
    17701 ggtgcgtgga tgtcgctatt ggcgaaaatc gccagacgat gttccttggc ccacgggaat
    17761 gcctccgggt agaccgttgt ctcattgacg agttcaaagc cctggatgag accgcgttcg
    17821 tgaagtgtcg atgcgggcgg ctcccactgc ggagtttcct tccatccggg gtgattccag
    17881 aagacgaacg catcctgctt ccgggccact tccagggctt ctgccattcc aaggctgaag
    17941 aatgcgttga agtctgtgac gaacagcgcg ttgcagtgga aatccttttc ggcgatctcc
    18001 acggcgggaa caaggatcag accgagttgc tcggccagcg gaagagcgat cgcgtgcggc
    18061 cggctgaggt cgggtttgag gtccttctcg tggggattgt agcccgcgtg gtcggtaatc
    18121 gcgatcgcgt ccaggccgtc gcgccaggct tcgacgacgc gggtggtggg ccacacttcg
    18181 ccatccgaga agacggtatg cgtgtggaag tcgcacttca gagttcgata gccggggaga
    18241 tcggggaccg gaagagcatc acgatgctgg gccgccgccg gtgcagcggc cagcacgagc
    18301 gcgaggagga agcgcattgg atcagtgaac cataatcgga gcaacagcga tggtcttgtc
    18361 ccacttcatc gtgatcgctc ccttcccgtt attggtcttt gaaatactga tggtgaactg
    18421 ctcaacttca ggcccttgac ttacctcgag cttggtacgg cccaggtctt gtgtttcctc
    18481 gtacttgtat gcaccccact gcttggcaac cttgttgaga atcagcatcc aggaggactg
    18541 gtcggggaga gtgaacaggc tgtagcttcc cttcggtacg tgaatggagc caaccatcaa
    18601 gtcggcttcc gttgtgagaa tggtcgcctc gtcggcgcca gtccgccaga ccttgccaaa
    18661 cgggacaagt cccccaaaaa tctcgcgccc tttcttgtac ggccggccgt actcgatgga
    18721 aattttcttg cctccgaaca cctcggtgac cgtttcatgc gggctgaccc gcttctgacc
    18781 ctgggccagg agaaccgcgc ccgcggtcaa accaacggca acgaacgaca gcagtgtgac
    18841 ggttttgcgc atcgagagga atctccttat gccgtcattc tatcaagcga ggaccgtagc
    18901 ttagcgaaca cgtaattttg cgaatcccgc agcaacgcac cgcccgtgtg gccgcaagtc
    18961 aggcagaatg ggtatgggac aacagtgaac ctgcgaacgc tatctttatt aattgccgtc
    19021 atccttgcgt attcccaagt ggctgctcag aagacggcag atgaatacgc cgcgcaaggt
    19081 ctgaaactgt tcgagaacgg acggtacgcc gaggcacgga aagatctaga ggcggccgtc
    19141 aaactagatc gcacccaggt ggaagcgctc ctactccttg tccgcatcca cttgatcgag
    19201 aaagactacg atggggcggt tgaactcgtg gctccactgg cccagtcgcc tgccgccgac
    19261 gccgaatcga gaatgctgct cgcctacacg ctggaaaaga ggggcgacct tcgccgcgcg
    19321 gctgggttgc ttgagcgaag cattgagatc atcacgtggg gcggagacgc cacctacgcg
    19381 gcggccgcac gggcgcgcat gaagctcggg gactatgagc gggctgcggc actctgcgag
    19441 cgggggatgg aatccagccc gaattcggag cgtcttctgc acacctacgt tagccttccg
    19501 gcggacatca tcggaaagcg ggttgccgac cggctcgcaa gagtcccgga cgagaaaggc
    19561 ggcgtgacag aacgaatcct cctcggccgg attctctcga agatcgatgt gaaatcattt
    19621 cctgacgctc gcgagaactc catgaagctg ctgacggagg ccgctacgcg ggagcctgaa
    19681 aactgctccg cccagtacaa cctcggacgg ctggagcgtc ttgtggggcg tacgaaggag
    19741 gccaaaggtg cgctcgagaa agccctaagc ttgaatccgg acgacgacct gcgaacattg
    19801 atccagacac agttggcgct cgcggaactg gcgtcgggcg atgatgctgc cgctgagcag
    19861 gcgtatcgtt ccgcacttga ctcgaaccga cggtctctgc aacctagtgc ggacgccgcc
    19921 ttggagtatg tccggttcct gaagctccgg tcacgagacg atgaggcaaa gaagctgctc
    19981 aatgaga
//
LOCUS       DATIFQ010000313         2982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2785990, whole genome shotgun sequence.
ACCESSION   DATIFQ010000313 DATIFQ010000000
VERSION     DATIFQ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2785990"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VLE22_14690"
     CDS             <1..286
                     /locus_tag="VLE22_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15703.1"
                     /translation="HGYSHFTSLHAPVRVGGLTVHPGDLLHADANGVTNIPREIAADV
                     ADAAAEFVSAEAAVLDYLKSGKPDPSGYAEARAEMVRRISALGGRLRGKK"
     gene            complement(402..968)
                     /locus_tag="VLE22_14695"
     CDS             complement(402..968)
                     /locus_tag="VLE22_14695"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSB15704.1"
                     /translation="MLARGQPNNTGKFLKDSLWLFAAYVRGQLLIALWMVVIYAVGYG
                     FAGVPWWGLVAPVCGALHLVPFLGAALGLILPVVVSLAAGGGTNQMLAILGVFVAAQA
                     IEGFYLTPKILGRQLRMRRVLVFVALVAGSSLFGFLGAIFAVPAVAVAVLAWRTWRGA
                     ETHVQDPPDLRNPTNRNTDPSTPSTSAQ"
     gene            1062..2330
                     /locus_tag="VLE22_14700"
     CDS             1062..2330
                     /locus_tag="VLE22_14700"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HSB15705.1"
                     /translation="MATMLTLEQETNPWQAAEIRFDEAASRLGLNDGLCKILRTPSKE
                     ITVYIPVMLDDGRIEVFTGYRVQHSVARGPGKGGIRFAPDVTLDEVRALASWMTWKCA
                     VVNIPFGGAKGGVICDPLLLSEGELERITRRYTAEILDFIGPERDVPAPDMNTNEKTM
                     AWVMDTYSMHMRHTVTAVVTGKPLDLGGSRGRSEATGRGCLIVTLQALKRLGIDPSSC
                     RVVVQGLGNVGGMAAKLMARQGFKIVSVIEIDGAIYNPKGLDIPALMEHRQHTGSILD
                     FPESENMSRKDAFFLECDVLLPAAKENVITSENAHRVRAKILCEGANGPTTPAADAIL
                     AGKGVFVIPDILANSGGVTVSYFEWVQDRQGFFWNEDLVNTRLEELMVNSFDQVVSYA
                     EKHNVNNRIAAYMLAIDRVAFAIKLRGIYA"
     gene            2327..>2982
                     /locus_tag="VLE22_14705"
     CDS             2327..>2982
                     /locus_tag="VLE22_14705"
                     /inference="COORDINATES: protein motif:HMM:NF013765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15706.1"
                     /translation="MSSDASTPLMRQYHSIKQQVPNTLLLFRLGDFYELFYEDAVTAA
                     RELEITLTSRNKEKGQAIPMCGVPYHAAEGYVARLIQRGYRVAICDQTEDPRFARKLV
                     KREITRIVTPGTATEPALLRSRENNYLASVIESAGRAGLAHVDVSTGEFRVTELDSGE
                     VSAVLENLGVREVLVPADSPTTPASAAAPGQGPLRTEVESWTFSSDYAGRTLREHFGL
                     "
BASE COUNT          546 a         1000 c          921 g          515 t
ORIGIN      
        1 ccacggctat agccacttca cgtcgttgca cgccccggtg cgcgtcggag ggttgaccgt
       61 ccaccccgga gatctgctgc acgccgacgc caacggagta acgaacattc cccgggagat
      121 tgccgctgat gttgcggacg ccgccgccga gtttgtctct gccgaggcgg ctgtgctgga
      181 ttacctgaag tcaggcaagc cggacccgag cggatacgcg gaagcccgtg cggaaatggt
      241 gcgccgcatc agcgcgctcg gggggcggct gcgggggaag aagtgaagcc gagggttctg
      301 atgatcctcg gcggggcgct tctggccagt gcgctcatga tggtggtttt gggtgagctc
      361 ctgcgcgggc aggcggacgg gatcgtggtc gccgtgctgc gctactgcgc gctcgtcgac
      421 ggtgtgcttg ggtcggtatt cctgttcgtg ggattgcgaa gatccggcgg gtcctgaacg
      481 tgcgtctccg cgccgcgcca cgtgcgccag gccagcaccg cgaccgcgac cgccgggacg
      541 gcgaaaatcg cgccgagaaa accgaacagc gagcttcctg ccaccagggc cacgaagacc
      601 agcacgcgcc gcatgcgcag ttggcggccg aggatcttgg gagtcaggta gaagccctcg
      661 atcgcttgcg cggcgacaaa cacccccaga atggcgagca tctggttcgt ccctcctccg
      721 gcggcaagcg agacgacaac cgggaggatg aggccgagtg cggccccgag aaacggaacc
      781 aggtggaggg ctccgcaaac cggcgccacc agcccccacc atggcacgcc ggcgaagccg
      841 tatccgaccg cgtagatcac taccatccac aacgcgatca gaagctgccc gcggacgtag
      901 gcggcaaaca gccacaggct gtccttcaag aatttgccgg tgttgttcgg ctgcccgcgc
      961 gcgagcaact tgacgcccct ttcttctccg agcgtagcat gatccgtccg cccttccgcc
     1021 ccggcgccac ccctgccata atgagggcgg aggctgacca gatggcaacg atgctcacgc
     1081 tcgaacagga gacgaatccg tggcaggcgg cggaaatccg ctttgatgaa gcggcttccc
     1141 ggctcggatt gaacgacggt ctctgcaaga tcctccgcac tccttcgaaa gagatcaccg
     1201 tctacattcc ggtgatgttg gatgacggac gtatcgaggt gttcaccggc taccgcgtgc
     1261 agcattcggt ggcgcgcggg ccgggcaagg gcggtatccg cttcgccccg gacgtgacac
     1321 tggacgaggt gcgcgccctg gcgtcctgga tgacctggaa gtgcgccgtc gtgaacattc
     1381 ccttcggagg cgccaagggc ggcgtcatct gcgatcctct gctgctttcc gaaggagaac
     1441 tcgagcgcat tacccgccgc tacacagccg agatcctcga cttcatcggc cccgagcgag
     1501 acgtgccggc gcccgacatg aacaccaacg agaagaccat ggcctgggtc atggacacct
     1561 actcgatgca catgcggcac accgttaccg ccgtcgtaac cggaaagccc ctggaccttg
     1621 gcgggtcgcg cggccgctcg gaggctaccg gcagggggtg cctcatcgtc acgcttcagg
     1681 cgctcaagcg gctcggcatc gatcccagct cctgccgcgt ggtcgtccag ggtcttggca
     1741 acgtcggcgg aatggccgcg aagctcatgg cgcggcaggg gttcaaaatc gtctccgtta
     1801 tcgagattga cggcgccatt tacaacccaa aaggactcga tatcccggcc ctcatggagc
     1861 accgccagca caccggctcc atcctcgatt tccccgaatc ggagaacatg agccgtaagg
     1921 acgccttctt tctcgagtgc gatgtgcttc tccccgccgc caaggaaaat gtcatcacgt
     1981 ccgagaacgc gcaccgcgtc cgcgcgaaga ttttgtgcga gggcgcgaac ggtccaacca
     2041 cccccgccgc cgacgccatc cttgccggta agggcgtctt tgtcatcccg gacatcctgg
     2101 ccaactccgg cggcgtcacc gtctcctatt tcgagtgggt acaggacagg caaggcttct
     2161 tctggaacga agacctcgtc aacacgcgcc tcgaggagct catggtcaac agcttcgacc
     2221 aggtcgtgtc ctacgccgag aagcacaacg tcaacaatcg catcgcggcc tatatgctcg
     2281 ccatcgaccg cgtggccttc gccatcaagc tgcgcggcat ctatgcatga gttctgacgc
     2341 ttcgacgccg cttatgcggc agtaccactc gatcaagcag caggtgccga acaccctgct
     2401 gctgttcagg ctcggcgact tttacgagct gttttacgaa gacgccgtca ccgccgcccg
     2461 ggagctcgag atcaccctca cttcccgcaa caaggaaaag ggccaggcca tccccatgtg
     2521 cggcgtgccc tatcacgccg cggagggtta tgtcgcgcgg ctgatccagc gcggttaccg
     2581 ggtcgccatc tgcgaccaga ccgaagatcc ccgttttgcc cgaaagctcg tgaaacgcga
     2641 gatcacccgg atcgtcacgc ccggcaccgc gaccgagccc gcgttgctgc ggtcccgcga
     2701 gaacaactac ctcgcgtccg tcatcgagag cgcgggccgt gccggccttg cccacgtcga
     2761 tgtctcgacc ggcgagtttc gagtgaccga actggactcc ggtgaggttt cggcggtcct
     2821 tgagaacctc ggcgtccgcg aagtgctcgt tcccgcggat tcgccgacca cccctgcctc
     2881 agccgccgca cccggtcagg gaccgctccg caccgaagtc gaatcgtgga ccttttcctc
     2941 cgactatgcc gggcgcaccc tccgcgagca ttttgggctt tt
//
LOCUS       DATIFQ010000314        14564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1444274, whole genome shotgun sequence.
ACCESSION   DATIFQ010000314 DATIFQ010000000
VERSION     DATIFQ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444274"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1436
                     /locus_tag="VLE22_14710"
     CDS             69..1436
                     /locus_tag="VLE22_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15707.1"
                     /translation="MKAAIVFVAAICTAQVRPAMDQNGQQLLAKVRATTGRALKSLPN
                     YVCLETTVRLQAPKLKAIAKAKALDTVELEVAHVGDKEMYSLPGRNMFADAQLTEVLP
                     SGLVGTGAYSSHLIDVLYGPGTQFRWVGKETLGGRAVLRWDFTVPKASSGWTLAARER
                     SVRVGSEGSLWADAATLSIVRLTSRATSLPARFPLKFATRTIDYASVHIGAGDVLLPS
                     AAEDVVEHSGGVNINRSRFGHCREFSTSSTVTFAEAGAPAPAPRQPAEPAPAASSAAQ
                     RGQGQQIAALNALPKGVRISLALDEHLRSENAVIGSAVNAHVRSDVRRSGAVLIPKGT
                     PVRGVLRDFVKVRDGFSSSMFTATIEFTELVLSTGAIPFDAFLKSVDVPVRGLYWLIP
                     GEEGTMKAACFRGPGSADPGDQFGRVRRKPKPGVAVLIFSDQPAFDLSPGTPMTWTTV
                     ESETR"
     gene            1441..1602
                     /locus_tag="VLE22_14715"
     CDS             1441..1602
                     /locus_tag="VLE22_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15708.1"
                     /translation="MSVSGLDVRVVDGDTISATNCVRQPFCSAQIGLAKAVVMVGRLN
                     LFWGLNWTA"
     gene            1658..1831
                     /locus_tag="VLE22_14720"
     CDS             1658..1831
                     /locus_tag="VLE22_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15709.1"
                     /translation="MDTRAARQVIATKVEGWLSRIVYWLDFGSSEASGQFFRGQPLNG
                     RNRRSKTAFRDLM"
     gene            2018..3157
                     /locus_tag="VLE22_14725"
     CDS             2018..3157
                     /locus_tag="VLE22_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15710.1"
                     /translation="MRNRVLLLACLLGTVIANAQRHKVNINTETPEGQILQQIGQEED
                     AAKKIALMEQFATQYPKHEASAWVYELMVPAYAKAGQFDKAMGAAEKLLALDPADAQG
                     AHEALKAAEAKKDPDAVKKWAVLTSEAARKVVQSKKGEDEEEEEFKQKIDFAKQVDVY
                     TEYSLYAMALQTTDPAKRAELAETLAERNPNSQYLPQVVPHLFSAYIQSGQGPKAVAL
                     AEKADASGQANEEMLLALAGSYRSEKKPPEKVLAYAEKTIQVVSAAQKPQGVSDADWE
                     KRKVAITGRANFIAGATHFAQNKFADADKVLRVALPGIKDDQQMTAEALFYLGVSNYR
                     LGEKGETERIKDALAFSQQCANIAGPFQAPARTNVKAIRSRYRVQ"
     gene            3154..4257
                     /locus_tag="VLE22_14730"
     CDS             3154..4257
                     /locus_tag="VLE22_14730"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HSB15711.1"
                     /translation="MNILVPNLGSTSLKYQILEMPAERVLSRGRLERVKDYREAVAQV
                     RTGDSRIDAVAFKAVHAGKKYRGTFVVDDELLAAMEEFLPAAPVHNAIYLTAMRAFQS
                     EMPGVPLVAAFETEFHATMPESAARYGVPVEWREKYGVRKYGFHGASHRYVGERVAEL
                     LGQPQPSLRLVSCHLGGSSSICAIDGGRSVDTTMGFSPQSGLENASRHGDLDVFAVLY
                     MMERRGWSIDEVRRQLASEGGLAGLSGVPGGDVRDLEQAAAAGNKDAALALEVFTYQV
                     KKTIGAYAAAMGGLDAVAFTGGIGENSPRLRAACCAGLEFLGMCLDPERNESGSGDRM
                     VSPDDSAVKILALATNEELIVARRAYGVLQAAH"
     gene            complement(4276..5331)
                     /locus_tag="VLE22_14735"
     CDS             complement(4276..5331)
                     /locus_tag="VLE22_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSB15712.1"
                     /translation="MATGTVAVQTSGADPGAGMPPAMAMMQLVCGKISTHLLYAMAKL
                     GVADHLNDAPQSAAELAEQIDADPSALYRALRALAGLGVFVEHEGKRFSLTPVGECLR
                     TDSPTSLRHLAIMFGEEWHVQAWGNILHSIRTGKPAAEDTLGMPVFEYFERDREQAGI
                     FNRAMTDLSKNAGKAVAEAYDYGGIRRLVDVAGGHGRLLTTLLDAHVHLEGILFDLPH
                     VIRSAREQAELAKYDGRLSFVAGDFFSSVPEDVDGYIMKHIIHDWDDERAARILSNCR
                     RGLRKNGKLLVVEQVVPADSAPSFAKMADIEMLLLPGGKERTEQEFRGLFAGAGFALT
                     RVIPTKSPVSIIEGTPA"
     gene            complement(5556..6032)
                     /gene="nuoI"
                     /locus_tag="VLE22_14740"
     CDS             complement(5556..6032)
                     /gene="nuoI"
                     /locus_tag="VLE22_14740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HSB15713.1"
                     /translation="MPSKINEVLQSASAIAKGMSVTFKEMMSPTVTENYPDEPPKFQE
                     RYRGVHVLRRDEEGMEKCVACFLCAAACPSHCIYIEAAENTAEERISGGERYAKVYNI
                     DYNRCIFCGYCVEACPTDAITHGHGFETASYHTSTLIYRKEQMLEPMPAAKSQAAD"
     gene            complement(6084..6557)
                     /locus_tag="VLE22_14745"
     CDS             complement(6084..6557)
                     /locus_tag="VLE22_14745"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB15714.1"
                     /translation="MLQAGAAAPAFRLRDLEGDDRSSEDLFAGGPVVLAFFKASCPVC
                     QYTLPFLDRMARGNPAGDLRIYVVSQDDARATRTFLRQYSISLPALLDAEESGYPASN
                     AFGISHVPSMFLIEPDGAIAWALDGFSKRDLEALGERAGVAPFAPGEHVPEWKAG"
     gene            complement(6587..7891)
                     /locus_tag="VLE22_14750"
     CDS             complement(6587..7891)
                     /locus_tag="VLE22_14750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB15715.1"
                     /translation="MRLVVIGGVAAGLSAASRARRLDRSLEILVLEKSPTISYAACGL
                     PYYIEGRVDSMRDLIVYTPEHFRRERNIEVRTGAEVASIHHPRRELSLAGGERVRYDH
                     LVIATGARPKRAGIEGAHLPHVFTLHTLTDAVRLREFIRNERPRRAAVIGAGYIGIEV
                     AEALRTQGLAVTLIESSEFVLNRADRELTETLRRHLERSRIDLRLNMRISGIESGRAG
                     DVPADLVVLSAGLVPNVEIAAEAGIQLGRTGAIEVSDRMETSLPGVYAAGDCAETTHL
                     ITGRPVYIPLGTTANKMGRIAGANAAGGRERFTGVVGTSIVRVCGLAVATTGLSAAEA
                     KREGFDSVSARIEARDRAPYFWPARTTVELVADRRTHRLLGGTVIGERDAAGRVNVIA
                     SALTSHMTIDDLENLDLAYAPPYAPVWDPLLVAAQQLHKLLD"
     gene            complement(7891..8295)
                     /locus_tag="VLE22_14755"
     CDS             complement(7891..8295)
                     /locus_tag="VLE22_14755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSB15716.1"
                     /translation="MRLEIEQREKEGVVVLDVKGGLVVGDPASALRDKLRQLLADERH
                     NVVLNLAGVDYIDSTGLGAMVVCFTSLRKAGGKLALLNLNRRQIELLVLTKLSTVFEI
                     FDDEQAAVNSFFPDREIRRFDILSFVQQQQDN"
     gene            complement(8328..9803)
                     /locus_tag="VLE22_14760"
     CDS             complement(8328..9803)
                     /locus_tag="VLE22_14760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB15717.1"
                     /translation="MSDGEPRVRITRRLRFRLTANYLLFFTLLFGALGAAFYHLLGRI
                     QDSQVRQLLDEQWLALVGYLRIRGSGPVWVFNQNDPNEVAAVERLRRVFFLADSQGQI
                     LEVSPGYRVIGVESPAEIRAAIQSRESGWRYRPDPAHVMYLLRWGVLVDAQRNRPYYV
                     AIGRSFDVTQPVRARFLWIYMAVLPLFVCGGSLFGWYWAGRALRPINNLVEATQRITG
                     TNLSLRIPASGTGDELDRLINTLNQMIERLEKSFRHMRQFTADVSHELRTPITAVRGQ
                     LEVALYTARTVEQYREAIMEALEEVERLTRFVHAMLSLSQAESGQLTLHKEAMDVVPI
                     LKDITGQFQIPADESRVQLSLETPPHCVAELDRVQFERLVTNLLANAVKFTPPGGEVR
                     VRLSADRDKLELVVEDTGCGIPAEYLPHVFDRLYRVPGIEGGPEKGLGLGLSFVSWIV
                     KAHAGSIRVASTVGKGTRFIVTLPLAAARAVDSTPLNVHPA"
     gene            complement(9796..10509)
                     /locus_tag="VLE22_14765"
     CDS             complement(9796..10509)
                     /locus_tag="VLE22_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB15718.1"
                     /translation="MRILVIEDERRIADFIARGLEGSGYTVEVAENGTAALARIHDID
                     YDLIILDLMLPDIDGLTVLQKVRNRKVSPPVLILSARGTLDDRVQGLELGADDYLVKP
                     FAFVELQARVRALLRRGQPLAERLQVGDLSLDCIRRKVARGDEVIDLAPKEFSILEYL
                     MRNKGRPLSRTMIVEHVWEMDYDGLTNIVDVYIRHLRSKIDEGRPQKLIHTVRGVGYM
                     VDAAEKPAVASPEPSRTDV"
     gene            complement(10575..11630)
                     /locus_tag="VLE22_14770"
     CDS             complement(10575..11630)
                     /locus_tag="VLE22_14770"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15719.1"
                     /translation="MDWTRGLAALIMLQGHVFHSFTHPDLRQDSPYVLSQFVGGMPPA
                     IFLFLTGVTLAFLMESRERQGLPGGARIIAALRRAGYLFGIAFLFRLQLWAFALPASP
                     WTDLFRVDILNCMGLSIAVLSLLAMFRTEQRVRVCAATGVLIAVAAPWISHFSWSSVP
                     SVLRAYFVPDTQFFAFFPWASFVAFGMSAGSILRIMRKDLMDRFMQWSAVVGFGLILG
                     AQYAANLPYSLYPASEFWLNSPTLILIKLGVILLMLAGAYLWTQYGAAPGWSWVRQFG
                     ITSLLVYWVHTELVYGRWLWFWKESLTSGQVAAAAVGVILLMLGLSVLRTNWNNLRGL
                     RLSYYYPYFIPGRASGD"
     gene            complement(11706..12296)
                     /locus_tag="VLE22_14775"
     CDS             complement(11706..12296)
                     /locus_tag="VLE22_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HSB15720.1"
                     /translation="MDKSASSFRDLCARLKRQDHTAVINDTTEETGKIQMRKVGKRGA
                     QMRIDFDAPDTRTAVFADRKAQIYYPKIQTVQIYDLGKYRELVDQFLLLGFGTSGQEL
                     QNNYLVRMTGEEKVSGEAVTRLELKPRAASAREHLDKVELWVAAGGYPVQQKFYRPSG
                     DYTLITYVDLKFNPGVSEQAVTLKLPSGVKREFPQK"
     gene            complement(12413..12772)
                     /locus_tag="VLE22_14780"
     CDS             complement(12413..12772)
                     /locus_tag="VLE22_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15721.1"
                     /translation="MVVSFWSGLKRFILWDYPRAGWQYDVMVGLILAFIFLTPKVWFR
                     DQPRPSSIVILQAHDGTDVVWLAPELLAQVPDAGKKDAAAELVRKRFGKQLRFARLDP
                     IFDAESEIKGYMATVRP"
     gene            complement(12826..13797)
                     /locus_tag="VLE22_14785"
     CDS             complement(12826..13797)
                     /locus_tag="VLE22_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15722.1"
                     /translation="MRLAVIGFGNVGRALGSLIERQRPSFPFRIVAIHTARHGTAYRD
                     DNSFTDPVFGPPAASVDEFLDLADAEVMVELTPLNPATGEPAISHVRAAFARRMHVVT
                     ANKGPIAHAYALLRDEARGAGVCFRFESTVMDGAPVFNLVRHTLPGVRVLGFTGVLNS
                     TTKIAIEAMRDGKSMQEGIEEARRLGITEADASFDIDGWDSAAKTAALANVLMDAMVT
                     PQQVDTKGIGRLTPERMAELAARRKTACLVSRARRTGDGIHLRVRAEVVEETDILASV
                     RGTSNLLLLHTDLMGTIGTVSISPSVAQTAYGVFSDLVGLLDCRSSV"
     gene            complement(13794..>14564)
                     /locus_tag="VLE22_14790"
     CDS             complement(13794..>14564)
                     /locus_tag="VLE22_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15723.1"
                     /translation="GPVTVFDSAAYGGEALMETLKASDRRLISYGVDLGTRITTQFDS
                     GRQILREVHFRRGVLTSRHAVQETRTYTIRNVDQKPKTLIVEHPVRPEYKLINAKPAE
                     TTATAYRFEVKLAPGANEKFPVVEERVFENVLAIVNLTPDVLASYVQNKELNEGARKQ
                     LERILQQKRLIAEVDQSIQAAEQQTNDLSRDQERLRQNINSLRQVTGQQEQVQEYARQ
                     LAANEIKLAGLRDQSFELRKKKAALESELNSLIEKMEF"
BASE COUNT         2872 a         4364 c         4632 g         2696 t
ORIGIN      
        1 agagccaagc gtggatactg ggcaacagca aattgacaaa caaatgttcc gggttgaatg
       61 aggacgcaat gaaagctgcc atagtattcg tagccgccat ctgtactgcg caagtaaggc
      121 ccgcgatgga ccagaatggc cagcagctac tggcgaaagt acgtgccaca acgggtcgtg
      181 cgttgaaatc gcttcccaac tacgtatgcc tggagacgac cgtccgcctc caagcgccga
      241 agttgaaggc gatcgcgaag gcgaaggcgc tcgacaccgt cgagctggag gtggcgcatg
      301 tcggggacaa ggagatgtat tccttgccag ggcgtaacat gttcgcggac gcgcaactga
      361 ccgaggtact gccctccggc ctggtgggga cgggtgctta ctcgtcacat ttgatcgacg
      421 tcctgtacgg gccgggcacg cagtttcgtt gggtgggcaa agagacgctc ggcgggcgcg
      481 cagtgctgcg ctgggacttt acggtgccga aggcctccag cggctggacg ctggccgcac
      541 gtgaacgttc ggtgcgcgtt ggctcggagg gtagcctctg ggccgatgcc gccaccttgt
      601 cgattgtgcg tctgacttcc cgcgccacat ccctgccggc gcgctttcct ctgaaattcg
      661 ccacgcggac tatcgactat gccagcgtgc acatcggagc cggcgatgta ttgctgccca
      721 gcgcggcgga ggacgtggtc gagcactcgg gcggggtgaa catcaaccgc agccgattcg
      781 ggcactgccg agagttctcg accagctcga ccgtcacttt cgcggaagcg ggtgcgccgg
      841 cgcctgcgcc tcgacaaccg gcggagcccg ctcccgcggc atcttccgcc gcacaacgcg
      901 gccaagggca gcagatcgcc gcgctcaacg cccttccgaa gggcgtgcgg atctccctgg
      961 cactggacga gcacctgcgc tcggagaacg ccgtcatcgg ttcggccgtg aacgcgcacg
     1021 tccgatcgga tgtccgtcgt agtggagccg tgctgatacc caaggggacg ccggtgcgcg
     1081 gcgtgctccg ggattttgtg aaagtcaggg acggcttctc gtcctccatg tttacagcga
     1141 cgatcgaatt cactgagctt gtgctttcca ccggagcgat cccatttgac gcgtttttga
     1201 agagcgttga tgtacccgtc aggggcctgt attggctgat cccaggcgaa gagggcacta
     1261 tgaaagcggc ctgcttcagg gggccgggga gcgcagatcc gggtgaccaa ttcgggaggg
     1321 taaggcgaaa accaaagcct ggcgttgcgg tcctgatctt cagtgaccag ccggcgtttg
     1381 atctctctcc gggcacgcct atgacgtgga ccacagtgga atccgaaacg agataaggaa
     1441 gtgagtgtta gcggacttga cgtgagggtc gtggatggtg acaccatctc ggcgacgaac
     1501 tgcgtccgtc agcctttctg ctcagcacag atcggtcttg ccaaggcggt cgtcatggtg
     1561 ggccggttga acctgttctg gggcctgaac tggacggcct agccgagcat ctgatacgca
     1621 gacagacttc tccaacttcg acgtcgtgat tggttgcgtg gacactcggg cggcacggca
     1681 ggtgattgcg accaaggtgg aggggtggct gagccgcatc gtatactggc ttgatttcgg
     1741 tagttccgaa gccagcgggc agttctttcg cggccagccg ttgaacggcc gcaatcgccg
     1801 ttcgaaaacc gcctttcggg acctgatgta gcacgcggga ggaattgtgg actttctgac
     1861 atcaggaatc cccggatcca tcacgaactc gcagaaaagg tccggggaaa gaagaagtcc
     1921 cggttcagcc tgtcggtcga gaaacgcatc gcccgggagt gagaagggcc ccggcggtga
     1981 tatgatagct tcccatccga gcatgggagg tgatgcgatg aggaaccggg ttttgctttt
     2041 ggcgtgcctg ctggggacgg tgatcgccaa cgcacagcgg cacaaggtaa acatcaacac
     2101 cgagacgcca gaagggcaga tacttcagca gatcggccaa gaagaggacg ctgcgaagaa
     2161 gatcgccttg atggagcagt ttgccacgca gtacccgaaa cacgaggcgt ccgcatgggt
     2221 ttacgaactg atggtgcctg cttacgcgaa ggctgggcag ttcgacaagg cgatgggagc
     2281 cgcagagaaa ctgcttgcgc tcgatccggc ggacgcccag ggcgcgcacg aagcgctgaa
     2341 agccgccgag gcgaagaagg acccagacgc cgttaagaag tgggccgtgt tgacatcgga
     2401 agcggcccgc aaggtggtcc agtccaagaa gggcgaggac gaagaggaag aggagttcaa
     2461 gcagaagatc gacttcgcga agcaagtgga tgtttacacc gaatactctc tttatgcgat
     2521 ggcgctccaa acaaccgacc cggcgaagag agccgaactg gccgagactc tggcggagag
     2581 aaacccgaac agccagtacc tgccgcaggt ggtgcctcac ctgttctctg cctatatcca
     2641 gtccgggcag gggccgaagg cggtcgctct cgccgaaaag gcggacgcga gtggccaggc
     2701 caacgaagaa atgctgctcg cgctggcggg cagctaccgg agcgagaaga agccgccgga
     2761 gaaagtgctg gcctatgccg agaagacgat ccaggtggtg agcgcggccc agaagcctca
     2821 gggtgtcagc gacgcggatt gggagaagcg gaaggtcgcg attacgggac gcgccaactt
     2881 catcgctgga gcgacgcact ttgcccagaa taagttcgcc gatgccgaca aggtgctccg
     2941 ggtggcgcta ccgggcatca aggacgacca gcagatgact gccgaggccc ttttctatct
     3001 cggggtctcc aactataggc tcggcgaaaa gggcgaaacc gaacggatca aggacgctct
     3061 cgcattcagc cagcagtgcg ccaacattgc cggaccgttc caggcgccag cgcgcaccaa
     3121 cgtgaaggca atccgctccc gctatcgcgt gcaatgaaca tcctggttcc gaatctcggc
     3181 tcgacgtcgc tcaagtacca gatcctcgaa atgcccgctg agcgggtgct gtcccgcgga
     3241 cgcctggagc gagtgaagga ctaccgcgag gcggtggccc aggtccgcac cggcgactct
     3301 cgtattgacg ctgtggcctt caaggcagtc cacgcgggca agaaataccg cggcacgttc
     3361 gtagttgacg acgagttgct ggcggctatg gaggaattcc tgccggccgc gccggtgcac
     3421 aacgcgatct atctcaccgc aatgcgagcc tttcagtctg agatgcctgg cgtccccctg
     3481 gtggcggcgt tcgagacgga attccatgcc accatgcctg aatccgcggc gcgctacggt
     3541 gtgccggtcg aatggcgaga gaagtacggc gtgcgcaagt acggttttca cggcgcctca
     3601 catcggtatg tcggcgagcg cgtagccgag ttgcttgggc agccccagcc atcgctgcgg
     3661 ctagtgagct gccacctcgg ggggagttct tcgatctgcg cgatcgacgg cggtcgctcg
     3721 gtggacacaa cgatgggctt ctccccccaa tctggcttgg agaatgcgag ccggcacggc
     3781 gacctggatg tcttcgcggt gctctacatg atggagcggc gcggctggag catcgacgag
     3841 gttcggcgac aactcgctag tgagggcggt ttggcgggac tctccggcgt gccgggcggc
     3901 gacgtccgcg acctggagca ggctgcggcg gcgggcaaca aggacgccgc gctggcgctc
     3961 gaggtcttca cctatcaggt gaagaaaact atcggggcgt acgcagcggc gatgggagga
     4021 ctcgacgccg tcgctttcac gggtgggatc ggagagaatt cgccgcggct acgggcggcg
     4081 tgttgcgccg ggctggaatt cctcggtatg tgccttgacc ccgagcgcaa cgagagcggg
     4141 tccggagacc gcatggtgtc tccggacgat tccgcggtca agattctagc gctggctacg
     4201 aacgaagagt tgatcgtcgc gcgacgggcc tacggggtgc tccaagctgc acactgacct
     4261 gggaagcaac cccgctcagg ccggcgtgcc ttcgatgatc gacaccgggc tcttcgtcgg
     4321 gatgacccgg gtgagagcga agcccgcccc tgcgaacagc ccccgaaatt cctgttcggt
     4381 gcgttccttg ccgcccggca ggaggagcat ctcgatgtcc gccatcttgg cgaacgacgg
     4441 cgcactgtcc gccggaacca cctgttcgac cacgagcagt ttgccgttct tgcggagccc
     4501 gcggcggcaa ttgctcaaga tgcgcgcggc gcgttcatcg tcccagtcgt ggatgatgtg
     4561 cttcatgatg tagccgtcga catcctcggg aacggacgaa aagaagtccc ccgcgacgaa
     4621 agacagccga ccatcgtact tcgccagctc tgcctgttcc ctggcgctcc gaatcacgtg
     4681 cggaaggtcg aacagaattc cctccaggtg aacgtgcgca tcgagcaagg tcgtgaggag
     4741 ccggccgtgc ccgccggcca cgtcgacgag gcgccggatt cccccgtaat cgtaggcttc
     4801 ggccacggcc ttcccagcgt tcttggagag gtcggtcatc gcgcggttga agatgccggc
     4861 ctgttcccgg tctcgctcga agtattcgaa aaccggcatt cccagcgtgt cttccgcggc
     4921 gggctttccc gtacggatgc tgtggagaat gtttccccag gcttgtacgt gccactcctc
     4981 accgaacatg atcgccaggt ggcgaagcga ggtgggcgag tcggtgcgga ggcattctcc
     5041 gaccggcgtc aaagagaacc gcttcccctc gtgttccaca aagacgccga gcccagccag
     5101 agcacgtagc gcacggtaga gcgccgaagg atcggcgtca atttgctctg ccagctcggc
     5161 cgcgctttga ggggcgtcat tcaggtggtc ggcgacaccc agcttcgcca tggcgtaaag
     5221 aaggtgagta ctgattttgc cgcagaccag ttgcatcatg gccatggccg gcggcattcc
     5281 ggcgcccgga tcggcgcctg acgtttgtac agcgactgtc ccagttgcca ttgagttgca
     5341 tcccttcggg ccgcgcggat tcgatcccgg aaaccagggc caaccccagg ccggccccgg
     5401 tcatgcacag cccctctgaa accatgatac gcgaaggaag gatggccggt cagcgagaag
     5461 tcgctccgcg ggaaggcggc agtgggcaaa cggaagcaag gtgggagtct gggttcaaaa
     5521 ggatggctag attgtccgca ggccggaacg ggctctcagt ccgcggcttg cgatttcgcc
     5581 gccggcatcg gctcgagcat ttgctctttg cggtagatca gcgtcgaggt atggtaactg
     5641 gcggtctcga aaccgtggcc atgggtgatg gcgtctgtcg ggcaggcctc gacgcagtat
     5701 ccacagaaaa tgcagcggtt gtagtcgatg ttgtagacct ttgcgtagcg ctcgccgccg
     5761 ctgatgcgtt cctcggccgt gttctccgcc gcctcgatgt aaatgcagtg ggacgggcac
     5821 gcggcggcgc acaaaaagca ggcgacgcac ttctccattc cctcctcgtc acgccgcagc
     5881 acgtggacgc cgcggtagcg ctcctggaac ttcggcggct cgtcagggta gttttccgtc
     5941 acggtaggag acatcatctc cttgaacgtg acgctcatcc ctttggcgat ggcgctggcg
     6001 gactgtaaca cctcgttgat cttcgaaggc atccctgttc ttagattatc gcagctccca
     6061 ccgggagcct agttcttgga gcctcaaccg gctttccact ccgggacgtg ctccccggga
     6121 gcgaacggcg caaccccggc gcgctcgccc aaggcttcga ggtcccgctt cgagaacccg
     6181 tccaaagccc aggcgatggc gccgtccggc tcaatcagga acatcgacgg cacgtgcgag
     6241 atgccgaagg cgttgctggc cgggtagccg gattcctccg cgtccaaaag ggccgggagc
     6301 gagatgctgt attggcggag aaacgtccgc gtagcgcggg cgtcatcctg agacaccaca
     6361 tagattcgca gatcgcccgc cggattgcca cgggccatcc ggtccagaaa cggcagagtg
     6421 tattggcaga cagggcagga ggccttgaag aacgcgagaa caaccggccc gccggcaaac
     6481 agatcttcgg acgaacggtc gtctccttca aggtcacgca ggcgaaatgc cggcgccgcg
     6541 gcccccgcct gcagcatctc tcgtgtgtgc cgccacccca tggccatcag tctaacagct
     6601 tgtgcagttg ctgagccgcc acgaggagtg ggtcccagac cggtgcatac gggggcgcat
     6661 aggcgagatc gagattttcg aggtcatcga tggtcatatg gctggtgagc gcggaggcaa
     6721 tgacgttaac gcggccggct gcgtctctct cgcctatgac cgtgcccccc agcagcctgt
     6781 gtgtccgccg gtcggccacg agttcgaccg tggtccgagc gggccagaaa tagggggctc
     6841 ggtcccgggc ctcgatccgt gccgagaccg aatcgaaacc ttcccgcttc gcctcggcgg
     6901 ccgacaaccc ggtagtcgcg accgcgagac cgcagacacg gacaatcgac gttccgacca
     6961 ctccggtgaa ccgttcccgc ccgcccgcgg cgttggcacc tgcgatgcgc cccatcttgt
     7021 tggcggtcgt cccgagcggg atgtaaacgg gcctgccggt gatgagatga gtcgtctcgg
     7081 cgcaatcgcc cgcggcatag acgccaggca ggctggtttc catccggtcg ctcacttcga
     7141 tggcgccggt acggccgagc tgaatgcccg cctccgccgc gatctcgacg ttcggcacca
     7201 ggccggccga cagcacgacg aggtcggccg gcacatcgcc ggcacggccg gactcgattc
     7261 cggagatgcg catattcagc ctcaggtcga tccgggatcg ctcgaggtgg cggcgcagcg
     7321 tctcggtcag ctcacgatcg gcgcggttta ggacgaactc cgaggactcg atcagcgtga
     7381 ccgcgaggcc ctgtgtgcga agggcctccg cgacctcgat gccgatgtag cccgctccga
     7441 tgacggccgc gcgtcgcggg cgttcgttcc gaatgaactc gcggagtcgg acggcgtccg
     7501 tgagcgtgtg cagggtgaag acgtgcggaa gatgtgcgcc ttcgatgccg gcgcgtttcg
     7561 ggcgcgctcc ggtggcgatc acgaggtgat cgtaacgcac gcgctcgccg ccggcgagcg
     7621 agagctcacg ccggggatgg tgaatggatg cgacctccgc tccggttcgt acttcgatgt
     7681 tccgctcccg gcgaaagtgc tcgggcgtat atacgatcag atcgcgcatc gaatcaacgc
     7741 gaccctcgat gtaatagggc aggccgcagg cggcgtagga gatcgtcgga cttttttcca
     7801 gcaccaggat ttcgagcgaa cgatcgagcc tccgggcgcg tgacgccgcg ctcagtccgg
     7861 ctgcaacgcc gccgatgact acaagccgca tcagttatcc tgctgttgct gaacgaagga
     7921 gaggatgtcg aagcgccgga tttcacgatc tggaaagaag ctgttgacgg ccgcctgctc
     7981 gtcgtcgaag atctcgaaca cggttgacag cttcgtaagc acgagcagct caatctgacg
     8041 gcgattcagg ttgaggaggg cgagctttcc accggccttc cgcaggctgg tgaaacagac
     8101 gaccattgct ccgaggccgg tactgtcaat gtaatcgacg ccggcgagat tgagaaccac
     8161 gttgtgccgc tcgtcggcga gcaactgccg gagcttgtcg cgcaatgccg aggcaggatc
     8221 acccaccacc aggccgccct tgacgtccag gacgacaaca ccctcttttt cccgctgctc
     8281 aatctccagg cgcatagcag gactctattg taataccgga agtccgctca tgcgggatgt
     8341 acattcagcg gggtggaatc gacggcccgt gcagcggcca gcggcagcgt cacgatgaaa
     8401 cgggtaccct ttcccacggt actggccaca cggatggaac cggcgtgcgc cttgacgatc
     8461 cacgagacga agctcaaacc aaggcccaat cctttttcgg gcccaccctc gatcccgggt
     8521 actcgataca gacggtcaaa gacgtgcggc aggtattccg cggggatacc gcagcctgtg
     8581 tcctccacga cgagctcaag cttgtcgcgg tccgcggaca gccggactcg cacttcgccc
     8641 ccaggaggag tgaacttgac cgcattagcg agaagattcg tgacgagccg ttcgaactga
     8701 acgcgatcga gctccgctac gcagtgcggt ggcgtctcca gggagagctg cacgcgggac
     8761 tcatccgccg gaatctggaa ctgacccgta atgtccttaa ggatcggtac gacatccatc
     8821 gcttccttat gaagggtgag ctggccggac tccgcctggg acagagagag catcgcatgg
     8881 acgaagcgag tcagccgttc cacttcctcc aacgcttcca tgatggcctc ccggtactgc
     8941 tccaccgttc gcgcggtgta cagggccacc tcgagctggc cgcgcacggc ggtgatcggc
     9001 gtgcgtagtt catgcgacac gtcggccgtg aactgacgca tatgccggaa cgatttctcc
     9061 aaccgttcga tcatctggtt gagcgtattg atgaggcgat cgagttcatc gcccgttccg
     9121 ctggccggga tgcggaggct gagatttgtg cccgtgatcc tctgtgtagc ctccacgagg
     9181 ttattgatcg ggcgcagggc gcgtccggcc caataccaac cgaacagact gccaccgcac
     9241 acaaaaagcg gcagaacagc catgtagatc cagagaaatc gtgcgcgaac cggctgggtt
     9301 acgtcgaagg accttccgat cgcaacatag tacggcctgt tcctttgggc gtcaaccagc
     9361 acaccccaac gcagcagata catcacgtgg gctggatccg ggcggtagcg ccaccccgac
     9421 tcccttgatt gaatcgcggc ccgaatttcg gcgggggatt ccacgccgat aacgcgatac
     9481 ccgggcgaga cctccaggat ttggccctgc gaatcggcga gaaagaacac gcgccggagg
     9541 cgctcgaccg cggctacttc gttcgggtcg ttctggttga agacccagac cggcccgctg
     9601 cccctgattc ggaggtaccc caccagcgcc agccattgtt cgtccagtag ttggcgcacc
     9661 tggctgtcct ggatacggcc gagaaggtgg tagaacgccg cgccgagcgc tccgaacagc
     9721 aaggtgaaga aaagcagata gttcgccgtg agccggaacc ggagtcggcg agtaatgcgt
     9781 acacgtggct cgccgtcaga catccgttcg ggagggctcg ggggacgcca ccgccggctt
     9841 ctccgccgcg tcaaccatat agccaacgcc gcggactgtg tgaatcaact tttgcgggcg
     9901 cccttcatca atcttgcttc ggaggtggcg gatatagacg tccacgatgt tcgtcaatcc
     9961 gtcgtagtcc atttcccaaa cgtgttcgac gatcatcgtg cggcttagcg gtcggccctt
    10021 gtttcgcatc aggtattcca ggatgctgaa ctccttcggc gccaggtcaa tcacctcgtc
    10081 gccgcgggcg acttttcgac gaatgcagtc caacgaaagg tcgcccactt gaagccgttc
    10141 cgcgagcggt tggccgcggc gaagcaacgc ccggacgcgt gcctgcagtt ccacaaacgc
    10201 gaagggtttg acgagatagt cgtccgcgcc gagttcgagc ccctggacgc ggtcgtccag
    10261 agtgccgcgg gcgctcagga tcaacaccgg cggagagacc ttccgatttc gaaccttttg
    10321 caggaccgtc agcccgtcaa tgtcgggcaa cataaggtcc aggatgatta gatcgtagtc
    10381 gatgtcgtga attcgagcga gggcagccgt cccgttttcg gcaacctcca cggtgtatcc
    10441 actgccttcc aagcctcgcg caatgaagtc cgcgatccgt ctttcgtcct caatgaccag
    10501 aattcgcatc ttgggtaaga gattatcagg tcttgaggat gatcggtagg gggaggggat
    10561 ccgggacccg ccggctagtc gccggaggcc cggcccggaa tgaagtacgg atagtagtac
    10621 gaaagccgca agccccggag gttgttccag ttcgttctga gaacggagag gcccaacatg
    10681 agcaggatga ctccgacagc cgcggccgcc acctgtcccg acgtcaggct ttccttccag
    10741 aaccagagcc accggccgta gacgagctcc gtgtgcaccc aatagacgag aagcgacgtg
    10801 atcccaaact gacgcaccca actccatccc ggcgcggcgc cgtattgcgt ccacagatac
    10861 gctcctgcca gcatgagcag aatcacgcca agcttgatca ggatcagggt cgggctgttc
    10921 agccagaatt cagatgccgg gtacagcgaa tacggcagat ttgccgcgta ctgtgcgccg
    10981 aggataagac cgaaacccac gacggcactc cattgcatga agcggtccat caggtccttc
    11041 cgcatgatgc ggaggatact gccggcgctc ataccgaagg cgacgaatga agcccagggg
    11101 aagaaggcga agaactgggt gtcggggacg aagtaggctc ttagaaccga aggcacgctc
    11161 gaccaactga aatgggatat ccaaggagcc gcaactgcga tgagcacgcc cgtagccgcg
    11221 caaacccgaa ctcgttgttc agtgcggaac atcgccagga gcgacaacac cgcgatggag
    11281 aggcccatgc agttgaggat gtctacgcgg aacaagtcgg tccacgggct ggccggcagc
    11341 gcgaaggccc agagctggag ccggaaaagg aacgcaattc caaacaggta gccggcgcga
    11401 cgcaacgccg cgatgatacg ggcgcctccg gggaggccct ggcgctcacg gctctccatc
    11461 agaaaagcga gcgtaacacc ggtaaggaac aggaagattg ccggaggcat gccgcccacg
    11521 aactgcgaaa gaacgtatgg ggagtcctgt ctcagatcag ggtgagtgaa cgaatgaaac
    11581 acatgacctt gcagcatgat cagcgccgca agaccgcggg tccagtccaa atatgcgagt
    11641 cgggaggatg atttggaagc agatgccata ctcagggaat aacggcctac ttgttgttat
    11701 tatacctact tttgcgggaa ctcacgcttg acgccggaag gcaacttcag tgtcaccgcc
    11761 tgctcggaga cccccgggtt aaatttcaag tcgacatatg taatgagcgt gtagtcccct
    11821 gacggccggt agaacttctg ttgcaccggg tatccgccgg ccgccaccca caattcaacc
    11881 ttgtctaaat gttcgcgtgc tgatgccgct cttggcttga gttcaaggcg ggtgaccgcc
    11941 tcaccggaga ccttttcctc gccggtcatc cgaaccaggt aattgttctg aagctcctgg
    12001 cccgaagttc cgaaccccaa cagcaggaac tggtccacca attcccgata ctttccgagg
    12061 tcgtagatct gaacggtttg gatcttcggg tagtagattt gcgctttccg gtcggcgaaa
    12121 accgccgttc gggtatccgg cgcgtcgaag tcgatccgca tctgggcgcc acgcttacca
    12181 accttcctca tctggatctt gccggtctcc tcggtggtgt cgttgattac ggcggtgtgg
    12241 tcctgccgct tgagacgggc gcacaggtcg cggaatgagg atgcggattt gtccatggag
    12301 gccaggacgt cctccaattc cgacgctgac agtttcgatg caggacccca gcagcacagg
    12361 cttagtatca agaggagaag aggcattcgc ggtttcaggg tagtcgcttt cgtcagggtc
    12421 tcacggtcgc catgtagccc ttgatctcgg actcggcgtc gaagatggga tcgagtcgcg
    12481 caaaacgcaa ctgttttccg aatcgcttcc gcaccagttc ggcggcggcg tctttcttgc
    12541 cggcgtcggg gacctgagcg agaagctcgg gcgcaagcca gacgacgtcg gttccgtcgt
    12601 gggcttgcag tatcacgatg ctggaagggc gtggctggtc gcgaaaccag acttttggag
    12661 tcaggaagat gaaggccagg atgagcccga ccatcacgtc gtactgccag ccggccctcg
    12721 ggtaatccca caggataaac cgcttcaggc cggaccagaa gctaacaacc atatccacca
    12781 tcagtttagc acgcagccga agaagtgcta ctatccgcta ccgagtcaca cggatgaacg
    12841 gcagtccagc aggcccacaa ggtcgctgaa gacaccgtag gcggtctggg cgacggacgg
    12901 ggaaatcgaa accgtcccga ttgtacccat gaggtcggtg tgcagcagga gcaagtttga
    12961 ggtgccacgc acggaagcga ggatatcagt ctcttcaaca acctcggcgc ggacgcgcag
    13021 gtgtatgccg tcgcctgttc ggcgcgcacg gctgactagg caggcggttt ttcgcctggc
    13081 ggcgagttca gccattcgct cgggtgtcag gcggccgatc ccctttgtat ccacctgctg
    13141 cggagtgacc attgcgtcca tcagcacgtt cgccagcgcc gctgttttgg cggcggaatc
    13201 ccagccatcg atgtcaaagc tggcatcggc ctcggtgatg ccaagccggc gggcttcctc
    13261 gattccctct tgcatgctct tcccgtcgcg catcgcttcg atcgcgatct ttgtggtcga
    13321 attcaggacg ccggtgaaac ctagtaccct cacgccgggc agcgtgtgcc ggacgaggtt
    13381 gaacacgggc gctccgtcca tcacggtgga ctcgaagcga aagcagacgc ccgcgccccg
    13441 cgcctcgtcg cgaagcaatg cgtaggcgtg ggcgatcgga cccttgttcg cggttacaac
    13501 gtgcatccgc cgggcaaaag ccgcgcgtac gtgggagatg gcaggctcgc ctgtcgccgg
    13561 gttgaggggc gtcagctcga ccattacctc ggcgtccgcc aggtcgagga attcgtccac
    13621 ggaagccgcc ggcgggccga acaccggatc tgtgaaagag ttgtcgtctc ggtacgccgt
    13681 accatggcgg gcagtatgaa tggcgacaat gcgaaacggg aaagacgggc gctgccgctc
    13741 gatgagcgac ccgagcgccc gtccgacatt gccaaaaccg atgaccgcga gtctcaaaac
    13801 tccattttct cgatcaggga attgagttcc gattctagcg cagctttctt cttccgtaat
    13861 tcgaagcttt ggtcgcgaag gccggcgagc ttgatttcgt ttgctgcgag ctggcgtgca
    13921 tattcctgaa cctgctcttg ctgtccggtg acctggcgca ggctgttgat gttctgacgc
    13981 aggcgctcct gatcgcggct gagatcgttc gtctgctgtt cggcggcttg gatggactgg
    14041 tcgacttccg caatcagccg cttctgctgg aggattctct cgagttgctt gcgcgcgcct
    14101 tcgttcaact ccttgttttg aacatagctg gccagtacat cgggtgtcag attgacgatc
    14161 gcgagcacgt tttcgaaaac tcgttcttcg actaccggaa acttctcgtt ggcgccggga
    14221 gcaagcttca cctcgaaccg gtaggcggtg gccgtagttt ccgccggctt ggcgttgatc
    14281 agcttgtact ccggccggac gggatgctcg acgatgagcg tcttcggttt ctggtctaca
    14341 ttccggatgg tataggtgcg agtctcctgg acggcgtggc gggaggtaag cacaccccgg
    14401 cggaaatgga cttcgcgcag aatctgccgc ccggagtcga actgggtggt aatgcgtgtt
    14461 cccaggtcga cgccataact gatgagccgc ctgtccgagg ccttcagcgt ttccatgagc
    14521 gcctcgccgc cgtacgcggc cgaatcgaag acggtcaccg gacc
//
LOCUS       DATIFQ010000315         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1635499, whole genome shotgun sequence.
ACCESSION   DATIFQ010000315 DATIFQ010000000
VERSION     DATIFQ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635499"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VLE22_14795"
     CDS             <1..188
                     /locus_tag="VLE22_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15724.1"
                     /translation="IERMEVLREMVRGAMLCSDATLHQSEDGWLVDGDPSKAITYCAV
                     SRAGDYGPLPSPPAFSQ"
     gene            368..604
                     /locus_tag="VLE22_14800"
     CDS             368..604
                     /locus_tag="VLE22_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15725.1"
                     /translation="MMFYRLHQSDRKEETTVAAIPTNQLKLFEPVTALPMRPPPLMDE
                     LIAAVGPLLQTLLKEIAAAEAGTVSATVTDEPSP"
     gene            601..2673
                     /locus_tag="VLE22_14805"
     CDS             601..2673
                     /locus_tag="VLE22_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HSB15726.1"
                     /translation="MSKIAADHLSRTAYVYVRQSTLTQVQNNLESQRRQYALATRARQ
                     LGWERVVVIDEDLGHSGGGAARAGFDKLLEAVTREEVGAVLSIEASRLARNGRDWHAL
                     LDFCIVVGTLIIDEDGIYDPRLANDRLLLGMKGTFSEMELATFRQRSLQARQQKAERG
                     EFYTLIPVGYVLTAGNRLEIDPDTRVREAIRLVFTKFRELGSARQVILWMRQEGIELP
                     TRQAGDSNGDIRWRLAGAGLRRFLTNPIYAGAYVWGRTRTVVRLQGGRRVVRKQRVRP
                     TGWQVLITGHHPGYIDWDEYLHNQQVLAENNNMRGEIVRGAVRSGSALLAGLLRCGHC
                     GRRLGIGTSGKGKNGGVLFRYKCRASAEDPTVTGLGCIGFGSLQIDRAVSAELLRVVA
                     PAGLAAALAAIEEKRDRNADKRRHMEMALQQARYETTLARRQYDAVDPDNRLVASELE
                     RRWNERLAAAQQLEDQLTALASDAPHIVSAAERARLLALGQDLPAAWNHPAASDEIKK
                     RILRAVIEEIVVQAENNKLKCMIHWKGGEHTAIDVRRFSSGRTRWQTDENTTQLITAL
                     ARVLPDKSIAAFLNRRGRRTAKGHTWTGHRVNTFRNQRGIAVYRPGERAERGEMTAEE
                     AAAELGVTRMTLSRLIRAKLLPARQVCPGAPYVIRRADLDGPRVRRASARSPVTADPR
                     QETLDFQS"
BASE COUNT          532 a          827 c          907 g          494 t
ORIGIN      
        1 atatcgagcg catggaggtg ctgcgcgaga tggtccgcgg cgcaatgctg tgcagcgatg
       61 cgacgcttca ccagagcgaa gatggatggc tggtggacgg cgacccgtcc aaagccatca
      121 catattgcgc agtatcacgg gctggagatt acggtccatt accgtcacca cccgctttct
      181 cgcagtaagc ttgtaatcgc gtacgcagtg cgcagcggtg tcggtctgtt tttcgtggtg
      241 caaaggccga acagccgggt tcttgccata ccgtcttgga tgaccgaacc gtgggcgggt
      301 gacttacggg tatgcgacct gccttgtctc tcgcacggcg cccttcagga cctgcggcgg
      361 ctgcttgatg atgttctatc gtctccacca gtccgaccgg aaggaggaga cgaccgtggc
      421 cgcgatccca accaatcaac tcaagctgtt cgagcctgtg acagcccttc cgatgcgacc
      481 gcctccgctg atggacgagc tgatcgcagc ggtggggccg ctacttcaga cccttctgaa
      541 agagatcgcg gccgccgaag cggggacggt ttccgccacc gtaacggatg agccgtcgcc
      601 atgagcaaga tcgccgccga ccacctgtcg cgaacggcct atgtctacgt gcggcagtca
      661 acgctgaccc aagtccagaa caatctggaa agccagcgcc gtcaatatgc gctggccaca
      721 cgagccaggc agctcggctg ggagcgggtc gtcgtcatcg acgaagacct tggtcattcg
      781 ggaggggggg ccgcacgggc aggcttcgac aagctgctgg aagcggtcac gcgcgaggag
      841 gttggcgcgg tgctgtcgat cgaggcctcg cggttggcgc gtaatggacg cgactggcat
      901 gcgctcctgg acttctgcat cgttgtgggg acgttgatca tcgacgagga cggcatctac
      961 gatccgcggt tggcgaatga ccgcctgctc ctgggcatga agggcacctt cagcgagatg
     1021 gaactcgcta cgttccgcca gcgctcgctg caggcgcgac aacagaaggc ggagcgcggc
     1081 gagttctaca ccctgatacc ggtcggctac gtgctcaccg ccggtaaccg cctggaaatc
     1141 gatcccgata cgcgtgtgcg ggaggcgatc cgcctggtct tcaccaagtt ccgcgaactc
     1201 ggcagcgccc gccaggtcat tctgtggatg cgccaggagg gcatcgaact tcccacccgc
     1261 caagcgggcg attcaaacgg cgacatccgt tggcgactgg cgggggctgg tcttcggcgg
     1321 ttcttgacca acccgatcta cgccggcgct tacgtctggg gacgcacacg cacggtggtt
     1381 cggctgcaag gtggcaggcg tgtcgtcagg aagcagcgtg ttcggccgac gggttggcag
     1441 gttttgatta ccggccacca tccgggttac atcgactggg atgaatacct gcacaaccaa
     1501 caagtcctgg cggagaacaa caacatgcgt ggtgaaatag ttcgcggcgc ggtacggtcc
     1561 ggatcagctt tgctggccgg cttgctgcgc tgtgggcact gcgggcgccg cctcgggata
     1621 ggcacgagcg ggaagggaaa gaacggcggg gtcctgttcc gctataagtg ccgagccagc
     1681 gccgaggatc cgacggtgac ggggcttggg tgcatcggct tcggtagcct gcagatcgat
     1741 cgtgccgtct cggcggagtt gcttcgtgtc gttgctccag cgggacttgc cgcagccctt
     1801 gcggcaatcg aagagaaacg ggaccgcaac gcggacaagc gacggcacat ggaaatggcg
     1861 ctgcagcagg cccgctacga gaccacactg gcacgccggc aatacgatgc ggttgaccca
     1921 gacaaccggc tggtggcctc cgaactcgag cgccgatgga acgagcggct ggcggcggcg
     1981 caacaactgg aggatcagct gacggcgctg gccagcgatg cgccgcacat cgtgagtgcc
     2041 gctgagcggg ctcgtctgct ggctcttggt caggatcttc ccgcagcctg gaaccatccg
     2101 gcggcatcgg acgagatcaa gaagcgaatc ttgcgagcgg tgatcgaaga gatcgtcgtc
     2161 caggccgaga acaacaagct gaagtgcatg atccattgga aggggggcga acacacggcg
     2221 atcgatgtga ggagattctc cagcgggcgg acacgctggc agaccgatga gaacacgaca
     2281 cagctcatca ccgcgcttgc ccgtgtcctt cccgacaagt ccattgctgc ctttcttaat
     2341 cgtagggggc ggcgcacggc caaagggcac acgtggaccg gccatcgggt caatacattc
     2401 cgcaatcagc gtggcattgc cgtctatcgc cccggcgagc gagccgagcg tggggaaatg
     2461 acggccgaag aagctgcagc cgaactcggt gtcaccagga tgacgctcag ccgtctcatt
     2521 cgcgccaagc tgctcccggc ccgtcaggtt tgcccaggag caccttacgt catccggcgc
     2581 gccgacctgg acgggccgag ggttcgtagg gcatcggcca gatccccggt aacagcagat
     2641 ccgcgtcagg agacccttga ttttcaatca tgaaaggaga tgcgcatcat gtcggccagc
     2701 tgtcgcatct gccgacgcat tgtcgctttc tcgcgttcat gccgcagtgg attttctgca
//
LOCUS       DATIFQ010000316         5856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_870342, whole genome shotgun sequence.
ACCESSION   DATIFQ010000316 DATIFQ010000000
VERSION     DATIFQ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870342"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VLE22_14810"
     CDS             <1..481
                     /locus_tag="VLE22_14810"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HSB15727.1"
                     /translation="ADEELHRHALENIEQADALLFGRVTYEMMEAAWRSPRTGAMPDW
                     TEPFARTIDAARKYVVSSTLDRVDWNAELVRGDLGKAVQQLKRESGKGLFVGGVKLPL
                     ALAEMGLIDEYELVVHPRIAGHGPTLFAGLSKHVDLKLVNRLDFGSGAVAMRYEPRG"
     gene            705..1049
                     /locus_tag="VLE22_14815"
     CDS             705..1049
                     /locus_tag="VLE22_14815"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB15728.1"
                     /translation="MKNEHAAPETRGVTVKLLASLDLGPELEGMAGRQLRMRMVTIEP
                     GGIFGPIHDHKDRSGMVYILQGTITDHRDGVAKEYGPGVGWPEDTNTTHWLENRGTTA
                     AVEISFDIVRQQ"
     gene            1306..1563
                     /locus_tag="VLE22_14820"
     CDS             1306..1563
                     /locus_tag="VLE22_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15729.1"
                     /translation="MSRNQAKAVLTELDKIGPADEGKLKQWLPANFKRFGIAGERVRK
                     LVVLPHSKELKEIAIILLTKPEDEAAAIAVTIKSSKSNSSE"
     gene            1789..2115
                     /locus_tag="VLE22_14825"
     CDS             1789..2115
                     /locus_tag="VLE22_14825"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HSB15730.1"
                     /translation="MPFYLSKFSYTPETWARLIGHPEDRRDAARAYIASVGGKLHGFW
                     YAFGARDGYTLWEAPDNVSMAAVALAIGAGGALSELETTVLLTVEETMDALRKAGQVR
                     YRPPGT"
     gene            2266..2751
                     /locus_tag="VLE22_14830"
     CDS             2266..2751
                     /locus_tag="VLE22_14830"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HSB15731.1"
                     /translation="MSTIAAARFGSVFSAGLVAGVLLGDRMGASFARPALSASSFVTF
                     QQILHRHFVWMMPILLGIAILSMATWLMLAHARPGSAEFTFVGLAELAFISIVVLTRA
                     INVPINVQLMTWDASSPPSGVMNTWVRWEQAHTARTVIAVLGFALLLLAFGTSRSQQA
                     A"
     gene            2995..3453
                     /locus_tag="VLE22_14835"
     CDS             2995..3453
                     /locus_tag="VLE22_14835"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSB15732.1"
                     /translation="MRRILEPPDPNFRARVKTSFSLQGFMATLGASLELIEPGEVHIE
                     LPFSTHLSQQHGYLHAGAIAAIADSANGYAALTLAPAGAEVLAVEFKINLMVPASAPR
                     FLARGRVLRPGRRLSTCIAEVVPIDGPDHEAIATMLSTISIRPGDPPGPA"
     gene            3558..3965
                     /locus_tag="VLE22_14840"
     CDS             3558..3965
                     /locus_tag="VLE22_14840"
                     /inference="COORDINATES: protein motif:HMM:NF039957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6010 family protein"
                     /protein_id="HSB15733.1"
                     /translation="MLVRALIVGIVGGALTTLIAWSPVIPDPLVFYSVTLAVIGAIYL
                     GFAFSDGRPSIVVLELLVAGVFIALALLGLWSAPTFIAVGLVLHGVWDLAHRPRGVTT
                     KLPSWYPSFCAAYDFVFAAAFLLLAHRFAGRAP"
     gene            4045..4368
                     /locus_tag="VLE22_14845"
     CDS             4045..4368
                     /locus_tag="VLE22_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15734.1"
                     /translation="MKRRSSCTCRPRDSKAVRALAFGTLALGALAAGALAVGALAIGA
                     ISIGRLAIGRSRIRRVDIDALVVRELRVTENLQLPPTPRSESNPSSPPGSPPNARPSD
                     VSRAP"
     gene            4589..5035
                     /locus_tag="VLE22_14850"
     CDS             4589..5035
                     /locus_tag="VLE22_14850"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSB15735.1"
                     /translation="MRNRLSLLIVTLLAVTGLCSAQTGAISKTKAKDQVLVDSSRTTW
                     EAYKSRNISAMKAVTAPEYVSFTQAGPSNLQQDIDTIQKLTIESYSLDDPKVTWLTKD
                     VAILRYKCDLKGSFDGKLFKPVYATEVWVNRGGKWQIVSYQETPVS"
     gene            5161..>5856
                     /locus_tag="VLE22_14855"
     CDS             5161..>5856
                     /locus_tag="VLE22_14855"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HSB15736.1"
                     /translation="MSEPTLDSLRERIRLLDQDLVARVAERVGLARQIGEIKRRQKLS
                     TADYAQERAVLERARTAAREHGLDPRVAEDLFARLIRASVIAQDEDSLRFAGLGVGKR
                     AVIVGGAGRMGRWLGRFLSAQGYETGVLDPAAAPEADAWGRREFASADLVVCSTPPAT
                     VVELYAAWSRKPPSGVVVDIASIKTPLIEPIRALQRSGCRVASIHPMFGPSTVLLREA
                     DIVICDTDDPEATA"
BASE COUNT         1036 a         1836 c         1882 g         1102 t
ORIGIN      
        1 cgcggacgaa gagttgcatc gtcatgcgct cgagaacatc gagcaggccg acgccctcct
       61 cttcggccgg gtgacttacg aaatgatgga ggcagcgtgg cggtcgccgc ggaccggagc
      121 gatgcctgat tggacggagc ctttcgcccg gacgatcgac gcggccagga agtatgtggt
      181 gtcgagcacc ctggaccggg tcgattggaa cgcggagctc gtgcgcgggg atctggggaa
      241 ggccgttcag cagctcaagc gggagtcggg taagggactg ttcgtgggag gcgtgaagct
      301 tccgctggcg ctggcggaga tgggactgat cgacgagtac gagctcgtgg tgcaccccag
      361 gatagccggc catgggccga cgttgttcgc cgggctatcg aagcatgtcg acttgaagct
      421 cgtgaaccgg ctggatttcg gctcgggggc ggtggcgatg cggtatgagc cgagagggta
      481 gccatccgtc gaagcgctgc gccagacagt ctggcgggtc aaggtcagga acgaggcagg
      541 actcttgttg acgagaattt cgacggcgcg gatattgacg cgtgctcgtg gctgtcggca
      601 ggcggcctaa cgagcgcgtg ctgcggggtc gctcatcgcc ttccgctttt cgctcgccgc
      661 tggcgcgccg ctaggcggcc cgaacgtaca gacaggagac gacgatgaaa aacgagcacg
      721 cggcgcctga gacgagaggc gttacggtga agctcctagc atcccttgac cttggccctg
      781 aactcgaagg catggcaggg cgccaacttc gaatgcgaat ggtgactatc gaacctggag
      841 ggatcttcgg accgattcac gaccataaag acaggtcggg catggtctac atactgcaag
      901 gaacgatcac tgatcatcga gatggagtgg ctaaggagta tgggccggga gtgggctggc
      961 ccgaggatac gaataccacg cactggcttg agaatagagg aacgacggcg gcggtggaga
     1021 tttcattcga tatagtcagg caacagtgaa gctcaggccc gccattcgcc ccagtcgccg
     1081 cctaacatgc gcgtgcagcg gactccctca ccgccctcgg cgctttcccg ccgctgacgc
     1141 gccacccgtt atacgctctt gtcggcgaag ggaggatttc aaatgaaggc tcggttcacg
     1201 tctggtgtga tcgcgtcgtt gcttgtcgcg acgttcgcgt aggctgcgac gaacctcaac
     1261 tcttccagga gcaatatcta ccgattgacc tatccgagcg atctcgtgag ccgaaatcaa
     1321 gcgaaggcgg ttctgacaga actggacaag ataggtccgg cagatgaagg gaagctgaag
     1381 cagtggctgc cggccaactt caagcgcttt ggcatcgcgg gcgagcgggt caggaagctg
     1441 gtcgtcttgc cccacagcaa ggaactaaag gaaatcgcga ttatcctctt gacaaagccg
     1501 gaagacgagg cggcagcgat tgcggtcacg atcaagtcca gtaaatccaa cagctcagaa
     1561 tgagatctcg acgcggcaac atcggtcggt gccgaaccgg cggctaacct ggcgttgcag
     1621 cggactcgcc gccgtcgctt tcgctcgggc cgctcgctgc gctcgttcgg cccgccgcgc
     1681 tcgttcggct cgccgctgaa cgcccggccg ttgggcgctt tacgtctcgg aaactgtgag
     1741 agccgaccta gtacagatcg gaaactcggt tagcgccagg gagacaaaat gccgttctac
     1801 ttgtcgaagt tcagctacac gcccgagacc tgggccagac tgatcggcca ccctgaggac
     1861 cgccgagacg ctgcccgagc ttacatcgcc tcggtcggtg ggaagctcca tgggttctgg
     1921 tacgccttcg gagcacgcga cggttacacc ttgtgggagg ccccagacaa cgtgtctatg
     1981 gccgcggtcg cgctggccat tggcgcaggc ggcgccctca gtgagctcga aacaacagtt
     2041 ctcctgacgg tcgaggaaac gatggatgcc ctgcgcaagg ctggacaagt ccggtaccga
     2101 ccccccggca cgtagcggat cccgcgtgaa gaatccaagc gcggcgccta acatggcgtt
     2161 gcagcggact cgccgccgtc gctttcgctc gcgccgcccg ctccgctctc tcgggccgcc
     2221 gctcgacacc caaccgttag gccgccaggg cacaaggaag cggccgtgag caccatagcg
     2281 gccgctcggt tcggctcggt cttctcggcc gggctggtcg ccggcgtcct cttgggggat
     2341 cgcatgggag cgagctttgc ccgccccgcg ctctcggcct cgagcttcgt gacttttcaa
     2401 cagatcttgc acagacactt cgtgtggatg atgccgatcc tgttggggat tgcgattctg
     2461 tctatggcga cctggttgat gctggcccat gctcgccccg gaagcgcgga gttcaccttc
     2521 gtggggctgg ctgaactcgc gttcatctct atcgttgtcc tcacccgagc gatcaatgtt
     2581 ccaatcaacg ttcagctcat gacgtgggat gcgtcctcac cgccctcggg agtcatgaac
     2641 acttgggtcc ggtgggagca ggcgcacacg gcgcgcacgg tcatagcggt cctggggttt
     2701 gcgctgctac tcttggcgtt cggaacttcg aggagccagc aggcggcctg acatggcgtt
     2761 gccgcggact cgccgccctc ggtatcgctc gggccgctcg ctcggttcgc cgctgaacgc
     2821 ccgactgctc ggcccggtcc catggacggg cacggaatca atggtgcgag acgaacccgg
     2881 cgtagcagcc gatgagctcc tcgctgtcgc tcggacttca cagccgccgg gcaagtatcg
     2941 ttgcaggccc ggaacgccca tcacgacttg cggtcgaggt agtctgaacc tctcatgaga
     3001 cggattctcg agcctcccga tccgaatttc cgagcgcgcg tcaagaccag cttttcactt
     3061 cagggattca tggcaacgct cggcgcttca ttagagctca ttgaaccggg cgaggtgcac
     3121 atcgagctgc cgttctcgac gcacctgtcg cagcaacacg gctacttgca tgccggagcg
     3181 atagcggcaa ttgccgacag tgcaaatgga tatgccgcgc tcaccttagc gcctgccggt
     3241 gccgaagtat tggcggtgga attcaagatc aacctcatgg tcccggcgtc ggcgccccga
     3301 tttctcgccc gcggaagagt tctcaggccg ggtcgtcggc tttcgacttg cattgcggaa
     3361 gtcgtcccga tagatggtcc tgatcacgag gccattgcca ccatgctctc gacgatcagc
     3421 atcagaccgg gtgatccgcc ggggcctgcc taaggcggcg ttacagcgga ctcgccgccc
     3481 tcgctgcgct cgagccaacg gttcggaccc tcgccgctga acactcgacc gacagatcgt
     3541 gaggaggagg tatcagaatg ctggttcgcg cactgatcgt ggggatagtt ggaggcgccc
     3601 tgacgacgct gattgcatgg tctccggtca ttccggaccc tctggttttc tactctgtca
     3661 ctctggctgt gattggcgcc atctacctgg gtttcgcatt ttcggacggc cgaccttcaa
     3721 tcgtggttct cgagctgctc gtagccggag tattcatcgc tcttgcgctg ctgggtctct
     3781 ggtccgctcc gacattcatt gcggtcgggc tggtcctgca cggcgtctgg gatctcgcgc
     3841 atcgaccccg tggagtgacg acgaagcttc catcctggta cccgtcgttc tgcgcggcct
     3901 acgacttcgt cttcgcagcc gcgtttcttc tcctggcgca ccggttcgca ggccgcgcgc
     3961 cctaaggtgg cgtcgcaatc ggaatcccga ggcgaggcgc cgttcgccga ttgacgctat
     4021 agagttgggc ccgtcggtgg ttcgatgaaa aggcgttcct cgtgcacctg tcgccccagg
     4081 gactcgaaag ctgtccgtgc tctcgcgttc ggcacgctgg ctctgggcgc gctggccgca
     4141 ggggcgctgg cggtcggtgc actggccatc ggcgcgatct cgattggccg cctggcaatc
     4201 ggccgctccc gaatacggcg cgtggacatc gacgcgctcg tcgttcgaga gctccgcgtc
     4261 accgaaaacc tgcagcttcc accgacgccg cgctccgaga gcaatccaag ttcgcctccc
     4321 ggctcgccgc cgaacgcccg gccgtcggat gtctcgcgcg cgccctgatg gggatgcccg
     4381 gcccgtggca gccccgggcg ctggccgtgc tcacaaggga gagccatacg ctcagttttc
     4441 ggccagcggc ggcgcggggt ggtccctctg gcagatgcgc tcgttcgcct tgacctgatt
     4501 ggccgcaggt aatacgctgc gcttccctcg ggcagcctcg aagcagccaa tgccggttgc
     4561 ttactcttcc gatggaaagg atcccaatat gagaaacagg ctctcccttc tcatcgtcac
     4621 gcttctggct gtaacgggtc tctgcagcgc acagaccggc gcgatctcta agacaaaggc
     4681 caaagaccag gtgctcgtcg atagctcccg cacgacatgg gaggcctaca agagcaggaa
     4741 catctcggca atgaaggcgg tcaccgcccc ggagtacgtg tcctttactc aggccggccc
     4801 gtccaatctt caacaggata tcgatacgat ccaaaagctc accatcgagt cttattcgct
     4861 cgacgatccg aaggtcacct ggctgacaaa ggatgtggcc attctccgct acaagtgcga
     4921 cctcaaagga tctttcgacg ggaaactgtt caagccggtt tacgccaccg aagtgtgggt
     4981 caaccgtgga ggcaagtggc agatcgtctc ctatcaagag accccggtga gctgatgcct
     5041 cgctctcgag gagcgattta gcaaccgcgt gaagcggagg cggcgcgcgt gattccgtgg
     5101 gccaacctgc ggcggagggc cgtcgcgtga cgctgcaacg gggtgtcaag aggagcatcc
     5161 gtgtccgagc cgactctcga tagcctgcgg gagcgcatcc ggctgctcga ccaggacctc
     5221 gtggcgcgcg tggcggagcg cgtcggtctc gcgaggcaga tcggagagat caagcgccgg
     5281 cagaaactct cgacggcgga ttacgcgcag gagagagcgg ttctggagcg cgcgcgaacc
     5341 gccgctcgag agcacggcct cgatccgagg gtggcggaag acctcttcgc gaggctcatc
     5401 cgagcgtcgg tcatcgcaca ggacgaggac agcctgcgtt ttgcgggcct gggcgtcggt
     5461 aagcgcgccg tcatcgtcgg cggcgccggc cgcatgggcc gctggctcgg ccgcttcctg
     5521 tctgcccagg gctatgaaac cggcgtcctc gacccggctg ccgcgccgga agcggacgcg
     5581 tggggccgcc gggagtttgc gtcggcggac ctcgtcgtct gctcgacacc tccggcgacc
     5641 gtcgtcgaac tgtacgcggc ctggtccaga aagccgccgt ccggagtggt cgtcgacatc
     5701 gccagcatca agactccgct gatcgagccg atccgggccc tccagcgctc cggctgccgc
     5761 gtcgcctcca tccatcccat gttcggccct tccaccgtcc tcctgcgcga agccgacatc
     5821 gtcatctgcg acaccgatga ccccgaagcg accgcc
//
LOCUS       DATIFQ010000317        13937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1636280, whole genome shotgun sequence.
ACCESSION   DATIFQ010000317 DATIFQ010000000
VERSION     DATIFQ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13937
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636280"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VLE22_14860"
     CDS             <1..303
                     /locus_tag="VLE22_14860"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HSB15737.1"
                     /translation="RAYLVSQNVPSEAIIVEPEGETTVHSTAAAAEIMRRMGLTSCIV
                     VSDGYHIFRAKKMLEFRGLTVHGSPRPSPARSELSQWWLYFRQAVGYALWSAGIRI"
     gene            complement(371..1132)
                     /locus_tag="VLE22_14865"
     CDS             complement(371..1132)
                     /locus_tag="VLE22_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HSB15738.1"
                     /translation="MSISAIIVDDERLACDELAYLLREFPDVEIVGTGKNGLEAVRMI
                     EDLEPDLAFLDVQMPGLDGLGVIQRVRERNLPTPFFVLATAYEQYAVPAFRLETMDYL
                     LKPVDKLRLTETIDRVRRLIQDRQKPASAEVPAVQRPSPQRTKLLVRSGNRNLIVDAN
                     DVIYATIDNGLITMVTTTIEGQSNYRTIEELQSNLDPDVFWRVHRSYLVNINRIKEVI
                     PWFKSSYQLRMEDKKGTEIPVSRVQTKRLRTLLKL"
     gene            1216..2535
                     /locus_tag="VLE22_14870"
     CDS             1216..2535
                     /locus_tag="VLE22_14870"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HSB15739.1"
                     /translation="MPATDNVRIAPAEGKLGVLIPGMGAVTSTFIAGLEAIKAGWAKP
                     IGSLTQMATIRLGKRTEGRSPRIKDFVPLAGLNDLVIGGWDIFDDNVYEAAMKAGVLE
                     RPLLEKVKEPLSAIRPMKAVFNQDYVRRLDGPNVKKGGTNMDKARLLIEDIENFRKSS
                     GASRLVMIWCGSTEVFHRSSDVHSTLEKFERGLASDNPEIAPSQIYAYAALKSGVPFA
                     NGAPNLTTDVPALLDLARDRQVPICGKDFKTGQTFMKTLISPGLKARMLGLTGWFSTN
                     ILGNRDGEVLDDPGSFKTKEETKLSALEYILQPDLYPDLYKHFDHVVRINYYPPRGDA
                     KEGWDNIDIFGWLGYPMQIKIDFLCRDSILAAPLVLDLVLFLDLAQRAGMKGIQEWLS
                     FYFKGPMTAPGLYPEHDIFIQLMKLKNTLRWMRGEELITHLGLEYYD"
     gene            2551..3522
                     /locus_tag="VLE22_14875"
     CDS             2551..3522
                     /locus_tag="VLE22_14875"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB15740.1"
                     /translation="MNVLVIGGTLFIGRLLVAGLLKLGHRVTVLHRKPVHDLGPKVGG
                     IQGDRNDPEAMRRVLAGRRFDAVFDNVYDWEHGTTASQVEATALACGDSIRRYVFMSS
                     VAAYVEGLDRWEEDPLVPDDHSDPYARNKAMSERVLFRLHEKTGFPAVTIRPPYVYGP
                     GNPFYREAFFWDRLRDRRPLIIPEDGSRLMHFVYVEDLVQACLRAMEAPEAVGQAFNI
                     ANERPLTQMELVRALAATAGMEARTVSIPRAKILEAGGHPLGPNLYFAVYFDMPPITE
                     NIGKARRLLGFQPTDFLKGLTETYEWYLQQPATRQDYRFEDRLLALA"
     gene            complement(3437..4942)
                     /locus_tag="VLE22_14880"
     CDS             complement(3437..4942)
                     /locus_tag="VLE22_14880"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB15741.1"
                     /translation="MKILLTGTTGYVGGRLLRKLEHSGHSVRCMARRPEFLRRRDGAG
                     VEVVAGDVLDKLSLQYALAGVHTAYYLVHSLASESSFEVEDRQAAHNFAAAAKEAGVS
                     RIIYLGGLAAPGSELSPHLRSRLQTGEDLRSTGVPVIEFRASIIVGSGSLSFEIIRAL
                     TERLPVMVTPRWVSTQAQPIAIENVLGYLVSALDLPGNESRVFEIGGSDQVSYGDIIR
                     EYARQRGLKRLLIPVPVLTPRLSSLWLALVTPVHARAGRPLINGLRNASVVTDDSALR
                     VFDIRPMGIRKAIERALQNEDLEFAQTRWSDALSASRGVRSFAGVRFGTRIVDSREIS
                     VSVPPAAAFAPIRRIGGRTGWYYGDLLWRLRGRVDSMFGGVGLRRGRRDREEVAIGDT
                     LDFWRVEAYEPDCRLRLLAEMRVPGRAWLEFEVHGATGASTIRQTAIFDPIGLAGLAY
                     WYALYPVHALIFRGMLRRIAKAALKPEREDGLRNDSPAGWPAAEDTTRRSQ"
     gene            5040..5231
                     /gene="rpmB"
                     /locus_tag="VLE22_14885"
     CDS             5040..5231
                     /gene="rpmB"
                     /locus_tag="VLE22_14885"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSB15742.1"
                     /translation="MAKVCEICGRGPQFGNRVSHAHNVTKRRWNINLQTVRAIVNGAA
                     KKIRVCTACLRNGKVQKAA"
     gene            5299..6159
                     /locus_tag="VLE22_14890"
     CDS             5299..6159
                     /locus_tag="VLE22_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSB15743.1"
                     /translation="MRFVTFRRSSGAPEPGVVLDQKVVGLAGAGFPDVLSIVSGAEEA
                     RARIDAWIAAPPAEAVTDLDSVTLMAPIPRPPKIICVGLNWVDHAMESGQEIPEVPTI
                     FSKFPTSVIGPGDKIVLPRSSTKPDYEAEFAFVIGKGGRYIPAFRWREHVFGYMNFND
                     VSARDYQTATSQWMIGKSFDTFAPMGPYLVSADEIPDPHDLDIMLTINGEVLQHSNTR
                     HLIFRIPDLISFLSSVFTLEPGDVVSTGTPAGVGVARKPPRFLRAGDDVVVRVQGLGE
                     LRNPVVSETP"
     gene            6239..7459
                     /locus_tag="VLE22_14895"
     CDS             6239..7459
                     /locus_tag="VLE22_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15744.1"
                     /translation="MSQPWKTDKWFTSPWNYLPEVRETLNFPEKIRIHDVTLRDGEQQ
                     TGIVFRRAEKVAIAKKLAAAGVHRIEAGMPAVSADDEAAIKEIVSLGLSSEIFSFARC
                     MPADVKLALDCGVKGIITEIPSSDHIIKNAYGKSMEWAIKSSIETTLAAKEAGLYTVF
                     FTIDSTRSDLDRYMDLVEKVATEGHMDAMTLADSFGGCTPQAISAVVRKLRARFKQPI
                     EIHCHEDFGLGVANTIAALANGASVAHVTVSAIGERAGNVPLEDTVLALRLLYGIDTG
                     IHTEEFYSLSKLVQELCGFSLPPNRPIVGDSLYHIESGIVTMFHRRCKDVEPMEYVPF
                     AADLVGRPGIDIALGKGSGLANIEEHLERRGKTATPEQTNDILARVKQTSIQKKGLLT
                     DAEFDGILASVLKG"
     gene            7467..8369
                     /locus_tag="VLE22_14900"
     CDS             7467..8369
                     /locus_tag="VLE22_14900"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HSB15745.1"
                     /translation="MFRSLDALVDAARRKGPVRIAVAAGHDPDVIEALKQAREMGLAD
                     GIFVGNSDKIRALAKSAWSDIPPDRIVHEPDDAAAARRAIALVREGEARLLMKGKIGT
                     ATLIRAVLDREAGLRGSRLLSQVIVFQVPGEYRLRIMTDAAINIAPSLEQKADICRNA
                     IDVAHAIGIEKPNVALLCALEFVNPDMPATVDAAGLTLMNRRGQIIGGYLEGPIALDA
                     PLSKFAAERKGIDSPLVENTDVFIAPDIEAANILYRSILYFAKGESGGIVVGAKVPLI
                     LLSRAETAETKIRSIAVGILAAAG"
     gene            8439..10097
                     /locus_tag="VLE22_14905"
     CDS             8439..10097
                     /locus_tag="VLE22_14905"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HSB15746.1"
                     /translation="MAQRRDVLLRLLLILGFALALGLAQIRDSLRPAFAASLDVAVMD
                     ASTGRITPARVYLFKDGRPSRLSPVDALLPLRVDLYYRERLWKQVARPKTLEVTASDQ
                     SHFFLLDGQASFDLPAGKYRLEAYRGIFFKPASVEFELKAGESRRVELKLQPVAPDKQ
                     SQWLSGDDHIHLVRDRQDNDIFLRWLQAEDLSVANFLQLQRQSDAGMQYGFGEAAEAR
                     LPGFSIRSGEEARSHFYGHINLLGLRELLRPVSVGEVYANSPEAYPFPLVMFERARRD
                     GCTVGYAHFDGSQKHSTLLMDLALGTIDFVEVFQFGVLKTDAWYQLLNAGLRVTGIAG
                     SDFPVPLGNRKPWPQVVPLLGPERTLVKASAGRSAYEAWAEGVRKGEVVVSNGPLLEL
                     EVNGKGPGASIAWEGSSSKAEGVATVTFHRPIEKLEIVVNGEVAAVRGGNGTEVTMSL
                     PFAVPLPGSSWIAARAKAKSEEGEPELLAHTNPVYLLRNGLPVSVQADRAEVAKKWEA
                     EAEYYRSSELTFQSDTHRRELLEKVERALAALRQPGNQVADAAR"
     gene            complement(10073..11461)
                     /locus_tag="VLE22_14910"
     CDS             complement(10073..11461)
                     /locus_tag="VLE22_14910"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB15747.1"
                     /translation="MRLNRRWFLGHAAVLPGTAAAAAPSAASSSDLARRLAGGLDELR
                     LYNSHEHLIPEKTRLEQAVDFFGLAGHYAVNDVISAGLAGDALATVRDEQAPAERRWA
                     AFEPYWNAARFTGYGQALAIAIKDIYGVDEISHGTISRINEVIRNRNKPGLYREVLRE
                     RAKIDWCLVDPYWNEKPARLDQDYFLLAQKFDGFVTPASRKDIERLEGIAGVSIHSLA
                     DHKRALAATFEQAIRVGMVSVKSTLAYRRDLKFEEVPDHDADLEFERLLKGGAETPAG
                     FRAHKVRPFRALEDHMFHQVVRLADAHKFPFQIHTGLLAGNACFVENTNPTLLANLFH
                     LYPGVKFDLFHIGYPYQEELGVLVKLFPNVYADFCWAHIISPPGSRRTLDEYLETVPV
                     NKIMGFGGDYRFPELTYAHAKIARSNVAHVLTAKVENGLFSEDQALEVGRLLLRDNAL
                     KLFPPQRAASAT"
     gene            complement(11434..12684)
                     /locus_tag="VLE22_14915"
     CDS             complement(11434..12684)
                     /locus_tag="VLE22_14915"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSB15748.1"
                     /translation="MNGARAKRSAKADSLERKQFRQQLALKNRALDATAEGITIADAR
                     RPDNPLIYANEGFERLTGYGATDVIGRNCRFLQGVGTDHETLERLRAAIREKREITVQ
                     LLNYRRDGTPFWNRLSITPVRGSDGEVTHFIGVQSDVTDQKNAADALEAANRAMRRDL
                     EAAAKVQQSLLPASLPKVHGINLAWLYQPCQELAGDSLNVFLLDDHDVGLYILDVSGH
                     GVAAALLSVTLSHFLLPLPGQSLLYDADRPVISTPSQVVEKLNARFPLDPRSPQFFTM
                     LYGVLDRRTDEFRYVLAGHTGPVYVPRDGEPTLLEAGGLPAGLFPGVRYEDQVIRLGV
                     GDRLYFYTDGIVESENAAGDDFGNERLLKALDSGRTAPLAESINGIIEEVVRWHGPGA
                     LADDASLLALEVCSKACDSTDGGF"
     gene            12720..>13937
                     /locus_tag="VLE22_14920"
     CDS             12720..>13937
                     /locus_tag="VLE22_14920"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSB15749.1"
                     /translation="MRLAVLGFLAVFVLWAQVPVLRLTQLAAGLTNPTDIQHAGDGTN
                     RLFVVQQNGIIRILSGGVLRAAPFLDIRNRTVGSGERGLLGLAFPPGYPAKRYFYVNY
                     TNLAGDTVIARYRTTADPNVADSTSETILLTIAQPYANHNGGQLRFGPDGFLYVGMGD
                     GGSGGDPQNNGQNLRTLLGKMLRLDVESDLARVLIPPDNPFVNDTAARPEIWAIGLRN
                     PWRFSFDRAKGDLWIADVGQNRAEEIDFQSASSLGGENYGWNQMEGMQCYRSGCNTAG
                     LILPVVEYVRSEGCSVTGGFVYRGRESLGLRGTYLYGDFCTGRIWGLRREGDLWSNRL
                     LLASGLSISTFGEDEAGEILVADYGGAIYRIGGPVAPFFTSAGVTNAASYASGLVAGS
                     LATIFAAGVKDDNG"
BASE COUNT         2577 a         4473 c         4196 g         2691 t
ORIGIN      
        1 cgcgcgtatc tggtcagcca gaacgtgccg tccgaagcca tcatcgtgga gcctgaagga
       61 gagaccaccg ttcacagcac tgccgctgct gcagagatta tgcgccggat gggactgacc
      121 agttgcatcg tcgtgagtga cggataccac atcttccgag ccaagaagat gctggaattc
      181 cgcggactga ccgtccacgg ttcgccccgg ccgtctcccg cgcgaagcga gctttcgcag
      241 tggtggctct acttccgcca ggcggtcggc tacgcgctct ggtcggcagg aatccggatc
      301 tgacacagcg ctttaggaac cgcaaccgtg agtgagcggc gggctgcagc ggggatgctc
      361 gtcttacgca ttacagcttc agaagcgtgc ggaggcgctt cgtctggaca cggctcaccg
      421 ggatctccgt acccttcttg tcttccatgc ggagctggta gctcgacttg aaccagggga
      481 tcacttcctt gatccgattg atgtttacaa gataggaccg gtggacacgc cagaacacgt
      541 ccgggtcgag gttggactga agctcttcaa tcgtccggta gttggattgg ccctcgatcg
      601 tcgtggtcac catcgtgatc aagccgttgt cgatcgtggc gtaaatcaca tcgttcgcat
      661 cgacgatcag gttccggttt ccgctccgaa ccagcaactt agtccgctgc ggtgacggcc
      721 gctggaccgc cggtacctcg gccgacgccg gtttctgcct gtcctgaatc agccgcctga
      781 cgcggtcgat cgtttcggtc agccgtagct tgtcgaccgg cttcaggaga tagtccatcg
      841 tctccaggcg gaacgcaggc acggcgtact gctcatacgc ggtggccagg acaaagaatg
      901 gggtcggcaa gttcctctcc cgcacgcgct ggatcacccc caaaccgtcc aagccgggca
      961 tttggacgtc caggaaggcc agatccggct ccagatcctc gatcatgcgg acggcctcca
     1021 gaccgttctt cccggttccc acgatctcga cgtcaggaaa ctccctgaga aggtaggcca
     1081 gttcgtcgca ggccagccgc tcgtcgtcga caatgatcgc gctgatggac atcgtcgcgc
     1141 cctctgtcat ttgagtgcta gtataacatt acacattgaa gggattgccc ttatacgaaa
     1201 aggagaagac ttaagttgcc cgccacagat aacgtccgca ttgctccagc agaaggaaaa
     1261 ctcggcgtcc tgatccctgg aatgggagcc gtcacctcta ccttcatcgc cggcctcgag
     1321 gccatcaaag ccggatgggc caagcccatc ggatcgctga cccaaatggc caccatccgg
     1381 ctgggcaagc gaaccgaggg ccgcagcccc cggattaagg atttcgtgcc attggctggc
     1441 ctgaacgacc tggtcatcgg cgggtgggac atcttcgatg acaacgtcta cgaagccgcg
     1501 atgaaagcgg gcgttctgga acgccccctc ctggagaagg tcaaagaacc cctgtccgcc
     1561 atccggccga tgaaagccgt cttcaatcag gactatgtcc gccgtctcga cggccccaac
     1621 gtcaagaagg gcggcacgaa catggataag gcgcggttgc tcatcgaaga tattgagaac
     1681 ttccgcaagt cctcaggagc gagccgtctg gtgatgatct ggtgcgggtc gaccgaggtt
     1741 ttccacaggt ctagtgacgt tcactcgacc ctggagaagt tcgaacgcgg actcgcgagc
     1801 gacaaccccg aaatcgcgcc cagccagatc tatgcttacg ccgcactgaa gtccggtgtg
     1861 ccgttcgcca acggtgctcc gaacctcacc acagacgtcc cagccctgct cgacctcgcc
     1921 cgcgaccgcc aggtaccgat ctgcggaaag gacttcaaga ccggccagac cttcatgaag
     1981 acccttatct cgccggggtt gaaggcccgc atgctcggac tgacagggtg gttttcaact
     2041 aatatccttg ggaaccgtga cggggaggtg cttgacgacc ccggctcctt caaaaccaag
     2101 gaagagacca aactctccgc cctcgagtac attctccagc cggacctcta ccccgatctc
     2161 tacaagcact tcgatcacgt ggtacgcatc aactactatc cgccgcgcgg cgatgccaag
     2221 gaaggctggg acaacatcga catctttggg tggctcggct atccgatgca gatcaagatc
     2281 gacttcctct gccgagactc cattctcgcc gccccgctgg tcctcgacct ggtgttgttc
     2341 ctcgaccttg cgcaacgcgc cggcatgaag ggcatccagg agtggctctc gttctacttc
     2401 aagggcccga tgaccgctcc cggcctctac cccgagcacg acatcttcat tcagctcatg
     2461 aagctgaaga acacgctccg gtggatgcgc ggagaggaat tgatcacgca ccttgggctc
     2521 gagtattacg actaagcatc atccactgcc gtgaacgtac tcgtcatcgg cggaacactg
     2581 ttcatcggcc ggttgctggt ggccgggttg ctcaagctcg gccaccgcgt cacggtcctc
     2641 catcgaaagc ccgttcacga cctcggcccc aaagtgggcg gcattcaagg ggatcgcaac
     2701 gatcccgagg ccatgcgccg agtgcttgcc ggccgccgtt tcgacgccgt cttcgataac
     2761 gtctacgact gggagcacgg caccaccgcg tcccaagtcg aggcgaccgc gctcgcctgc
     2821 ggggattcca tccggcgcta cgtcttcatg tccagcgtcg ccgcatacgt cgagggtctc
     2881 gatcgctggg aggaggaccc gctggtccct gatgaccact ctgaccctta cgcccgcaac
     2941 aaggcgatga gcgagcgcgt gttgttccgg ctgcacgaaa agactggctt tccggccgtc
     3001 accatccgcc caccgtatgt gtacgggcca gggaacccct tctaccgcga ggcgtttttc
     3061 tgggaccggc ttcgcgaccg tcggccgctc atcattcccg aagacgggag ccggctgatg
     3121 cacttcgtct atgtggaaga ccttgtgcag gcgtgtctcc gggcgatgga ggcgccggag
     3181 gcggtgggac aggcgtttaa catcgccaat gagcgtccat tgacgcagat ggaattggtt
     3241 cgcgctctgg ccgcgacagc cggcatggaa gcccggacgg tctccatccc cagggcgaag
     3301 attctcgagg ccggcggcca cccacttggc ccgaatctct atttcgctgt gtacttcgac
     3361 atgccgccaa tcaccgaaaa catcggtaag gcccggcgac tgctcggctt ccagcccacg
     3421 gacttcctta aaggactcac tgagacctac gagtggtatc ttcagcagcc ggccacccgg
     3481 caggactatc gtttcgaaga ccgtcttctc gctctggctt aagcgccgct ttggcgatgc
     3541 gccgcagcat tccccgaaaa atcagcgcgt gcaccgggta aagggcgtac cagtaggcca
     3601 agcccgccaa cccgattggg tcgaagatcg cggtttgccg gatggtcgag gcgcccgtcg
     3661 cgccgtgaac ttcgaactcg agccacgccc gtcccggcac cctcatctcg gcgagcaacc
     3721 tgaggcggca gtccggctcg taagcctcca ctcgccagaa gtccaacgtg tcgccgatcg
     3781 ccacctcctc ccgatcccgg cgccctctcc gcaggcccac cccgccaaac atagagtcga
     3841 cgcggcctct cagccgccat agtagatcgc cgtagtacca gccggttctg ccaccgatgc
     3901 gacggatggg cgcgaacgcg gctgccggcg ggacggaaac cgaaatctcc cgtgaatcga
     3961 cgatgcgcgt gccgaaacga actccggcga atgagcgcac gccgcgagag gcggaaagcg
     4021 catccgacca gcgcgtctgg gcgaactcca ggtcttcgtt ctgaagcgcc ctctcgatcg
     4081 ctttgcggat ccccatcgga cggatgtcga acacacgcag cgctgagtcg tccgtcacca
     4141 ccgacgcgtt gcggagcccg ttgatcagcg ggcgcccggc acgggcgtgc accggagtga
     4201 ctagtgcgag ccagaggctc gacaggcgcg gcgtcaacac gggaaccggg atcaggagcc
     4261 gtttcaggcc tctctggcgg gcgtactcgc ggatgatgtc accgtaggag acttggtccg
     4321 agccgccgat ctcaaacacc cggctctcgt ttccaggaag gtccagtgcg ctgacgaggt
     4381 acccgagcac gttctcgatc gcgatgggct gtgcctgtgt cgagacccaa cggggcgtca
     4441 ccatcaccgg cagccgctcg gtcagggcgc ggatgatctc aaacgagagg ctccccgatc
     4501 cgacgatgat cgacgcccgg aattcaatca ccggaacgcc ggtggatcgg agatcctcgc
     4561 ctgtctgaag ccggctccgc aagtgcggcg agagctcgct gccgggtgca gccagtcctc
     4621 cgaggtagat gatccgtgaa actccagcct ccttcgctgc cgccgcgaag ttgtgggcag
     4681 cctgccggtc ttccacctca aaggagcttt cactggccag cgagtggact aggtagtagg
     4741 cagtgtgaac acccgccaat gcgtattgga ggctgagttt atccaggacg tcccctgcca
     4801 cgacctccac cccggcgccg tcgcgccgcc ggaggaactc gggccgtcgc gccatgcaac
     4861 gaacggaatg cccgctatgc tccaatttcc tgagcagccg gcccccgacg tagcccgtgg
     4921 tgcctgtcag caggatcttc atccgatctt ccgccctctt ccctttgatg ccggttgcgt
     4981 ccgctttgtc gcggctgcta aaatcaatat tcccactatt ttgaggagat tccccgtcga
     5041 tggcaaaagt atgtgagatt tgtggccgcg gccctcagtt cggcaaccgg gtcagccatg
     5101 cccataacgt aaccaagcgg cgctggaaca tcaacttgca gaccgttcgc gcgattgtga
     5161 acggcgctgc aaagaagatc cgcgtctgca cggcctgcct caggaacggc aaggttcaga
     5221 aggccgcctg agccttccgg attggctgcg gcacgttccc attctgcagt ctgggtataa
     5281 ctctacaccg cgatcagcat gagatttgtc acgtttcgcc gttcttccgg cgcgcctgag
     5341 ccgggcgttg tcctcgatca gaaagtcgtc ggtcttgcag gcgccggatt tccggacgtc
     5401 ctttccattg tgtctggcgc cgaggaagcc cgtgcgcgta tcgatgcatg gatcgccgcg
     5461 cctcccgcgg aggccgtgac ggacctggat tcggtgacgt tgatggcgcc gattccgcgt
     5521 cccccgaaga tcatctgcgt gggtctcaat tgggtcgatc acgccatgga gtcgggtcag
     5581 gagattcccg aggttcccac gatcttctcc aagttcccga cttctgtcat cggtcccggc
     5641 gacaaaatcg tgctgccccg atcttctaca aagcccgatt acgaagcgga gttcgcgttc
     5701 gtcataggca agggcggccg gtacatcccg gccttccgct ggcgcgagca cgtcttcggc
     5761 tacatgaatt tcaacgacgt cagcgcccgg gactaccaga ctgcgacttc gcagtggatg
     5821 atcggcaagt ccttcgacac cttcgcacca atgggaccct atctcgtttc ggcggacgag
     5881 atccccgacc cgcatgacct tgacatcatg ctcaccatta acggggaagt gctccagcac
     5941 tccaatacgc ggcacctgat attccgtatc ccggacttga tctcattcct gtcgagcgtc
     6001 ttcacgctcg agcccggaga tgttgtctcc accggcactc ccgccggcgt cggcgtcgcc
     6061 cgcaagccgc cccgtttcct gcgcgccggg gacgatgtcg tcgtgcgagt ccagggcttg
     6121 ggtgagctcc ggaaccccgt cgtctcggaa acgccgtagt ccggccttct ccttccaaca
     6181 tccggcgtcc gtccgccgat ataatgtcgg cttaccgaca aacgcaagga gcgtattcat
     6241 gagtcagccc tggaaaaccg acaagtggtt caccagcccc tggaattatc tgcccgaagt
     6301 ccgagagact ctcaacttcc ccgagaagat caggattcac gacgtcacgc tgcgcgacgg
     6361 cgagcagcag accggcatcg tcttccggcg cgcggagaaa gtcgctatcg cgaagaaact
     6421 ggcggccgcg ggcgtccacc gtatcgaggc gggaatgccg gccgtctccg ccgatgacga
     6481 ggccgccatc aaggaaatcg tctcgctcgg tctttcttcg gaaatcttct ctttcgcccg
     6541 ctgcatgcca gccgacgtga aactggctct cgactgcggc gtcaagggca tcattaccga
     6601 aatcccgtcc agcgatcaca tcatcaagaa cgcctacggc aagtcgatgg agtgggccat
     6661 caagtcgtcg atcgagacta cgctcgccgc caaggaagct ggcctttaca ctgtcttctt
     6721 cacaattgac agcactcgca gcgaccttga ccgttatatg gaccttgtcg agaaggtcgc
     6781 caccgaaggc cacatggacg ccatgacgct ggctgactcc tttggcggct gcaccccgca
     6841 ggcgatcagc gcggtggtcc ggaagctgcg cgcgcgcttc aagcagccca tcgagattca
     6901 ctgccacgag gacttcggcc tcggtgtcgc caacaccatc gccgcgctgg cgaacggcgc
     6961 ttcggtcgcc cacgtcaccg tgtcggcgat cggcgaacgc gccggcaacg tgcctctcga
     7021 agacaccgtg ctcgctctgc gtctcctgta tggcatcgat accggcatcc acaccgagga
     7081 gttctacagt ctctcgaagc tagtccagga gctgtgcggg ttctcgctgc ctcccaaccg
     7141 gcccatcgtt ggcgactcgc tgtatcacat tgagtccggc atcgtcacca tgttccaccg
     7201 ccgctgcaag gatgtggagc cgatggaata cgtccccttc gcggcggacc tcgtagggcg
     7261 tccgggtatc gacatcgctc tcgggaaagg gagcgggctg gcaaacatcg aggagcacct
     7321 cgagcgacgc gggaagacgg ccacgccgga gcagaccaac gacattctcg ctcgcgtcaa
     7381 gcagacatcg atccagaaga aaggtctgct caccgacgct gagttcgacg ggatcctcgc
     7441 ctccgttctg aaaggatgat ggcgccgtgt tccgaagcct cgacgcgctg gtagacgcgg
     7501 cccgccgcaa ggggccggtc cgtattgccg tggcggccgg gcacgacccg gacgtcatcg
     7561 aagcgcttaa acaggcccgc gaaatgggtc tcgccgacgg aatcttcgtc ggcaattccg
     7621 ataagatccg ggcgctcgcc aagagcgcct ggtccgatat tcccccggac cggatcgtac
     7681 acgagcctga cgacgcggcc gccgcccgcc gcgcgattgc cctcgtgcgc gagggcgaag
     7741 cgcgcctgct gatgaagggc aagatcggca ccgcgaccct catccgcgcc gtgctcgaca
     7801 gggaagccgg ccttcgcggc agccgtttgc tcagccaggt gatcgtcttt caggttccgg
     7861 gcgaataccg cctgcgaatc atgaccgacg ccgccatcaa catcgcgccg tcgctcgaac
     7921 agaaagcgga catctgccgg aacgcgatcg atgtggcgca cgctatcggc atcgaaaagc
     7981 cgaacgttgc tttgctgtgc gcgctggagt tcgtcaatcc ggacatgccg gcaaccgtcg
     8041 acgccgccgg tctgactctg atgaaccgcc ggggccagat catcggcgga tatctcgaag
     8101 gcccgatcgc tctcgacgct ccgctgagca agttcgctgc cgagcgcaag ggaatcgaca
     8161 gcccgctggt cgaaaacaca gacgttttca tcgcgccgga catcgaggcg gcaaacatac
     8221 tctatcgctc gatactgtat ttcgccaagg gcgagtcggg cggcatcgtg gtgggtgcga
     8281 aggtcccgct gattctgctc tcccgcgccg aaactgctga gaccaagatt cgctccatcg
     8341 ctgtcggcat tctcgccgcc gcgggatgat accgcttcct gtggtcgggg ctcggaatgc
     8401 tgcggcccgg agcggcgcca gttgtcgcga ggtgtgacat ggcacaacgt agagacgttc
     8461 tgcttcgctt gttgctcatc ctcgggttcg cccttgccct cggcctggcg cagatccggg
     8521 attctcttcg gcctgctttc gccgccagcc ttgacgtcgc ggtgatggac gccagtaccg
     8581 gcagaatcac cccagcccgc gtctacctct tcaaggacgg gcgtccgtct cggctgagtc
     8641 ctgtggatgc cctcctcccg ctccgggtgg atctctacta ccgcgagcgt ctctggaagc
     8701 aggtcgcccg gccgaaaacg ctcgaagtca ccgccagcga tcagtctcac ttctttctgc
     8761 tcgacggcca ggcaagcttc gacctgcctg cggggaagta ccgccttgaa gcgtatcgtg
     8821 ggatcttctt caagccggcc tcggtggagt ttgaactgaa ggcaggcgag tcgcggcgag
     8881 tggaactgaa gcttcagccc gttgcaccgg acaaacagag ccagtggctc tccggcgacg
     8941 accacattca cctcgtgcgg gatcggcagg acaacgacat tttcctccgc tggctgcagg
     9001 cggaagacct ctcggtggcg aacttccttc aattacagcg ccagtcggac gcgggaatgc
     9061 agtatggttt tggggaagcc gccgaagcgc gcctgcccgg cttctccatc cgctccggcg
     9121 aagaggcccg cagccatttc tacgggcata tcaacctgtt aggtctgcgt gaactcctgc
     9181 ggccggtcag tgtcggcgag gtttacgcca attcgccgga ggcctatcct tttccgctcg
     9241 tcatgtttga gcgcgcccgg cgcgacggtt gcaccgtcgg ttacgcgcat ttcgatggca
     9301 gccagaaaca ctcgacgctc ctcatggacc tggcgctcgg cacgatcgat ttcgtcgagg
     9361 tctttcagtt cggcgtgctc aagacagatg cctggtacca actcctcaac gccggcctgc
     9421 gggtcaccgg catcgctggc agcgattttc cagttccgct gggcaaccgg aagccatggc
     9481 ctcaagtcgt gccgctgctc ggtcccgaac gtacgctggt gaaggcatcc gccggccgct
     9541 ccgcatacga ggcctgggct gagggagtcc gcaaaggcga agtcgttgtc tccaatggcc
     9601 ccctgcttga actggaagtg aacggaaagg gcccaggcgc ttctatcgcg tgggaggggt
     9661 cttcaagcaa ggccgagggc gtcgcgactg tcacgtttca ccggcctatc gagaagctcg
     9721 aaattgttgt gaacggcgaa gtcgccgcgg tgcgtggtgg aaacgggacg gaggtgacca
     9781 tgtcgcttcc cttcgcggtc ccgctccccg gaagttcgtg gatcgctgcc cgcgcgaaag
     9841 cgaaaagcga ggagggagag cctgagctgt tggctcacac gaacccggtc tatctgctgc
     9901 gcaacgggct gccggtttcc gttcaggccg accgcgccga ggtcgcgaag aaatgggagg
     9961 cggaggcaga gtactacagg agctctgaac tgaccttcca aagcgacaca catcgccgcg
    10021 aacttttgga gaaggtcgaa cgagcgctgg ctgcactgcg gcagccggga aatcaggtgg
    10081 cggacgccgc ccgctgaggc gggaacagct tcaacgcgtt gtcccgaagc aacagccgcc
    10141 cgacttcgag ggcctgatcc tcgctgaaca gtccgttctc caccttcgcc gtcagcacgt
    10201 gcgccacgtt gcttcgtgcg atcttcgcgt gggcgtaggt aagctcgggg aaacgatagt
    10261 cgcctccgaa gcccatgatc ttgttcaccg gcaccgtctc aaggtattca tcgagcgtcc
    10321 gccggctgcc cggaggcgag atgatgtggg cccagcaaaa gtcggcgtaa acgttcggga
    10381 acagctttac cagcacaccg agctcttcct ggtacgggta gccgatgtgg aacagatcga
    10441 atttcacgcc tgggtagagg tggaatagat tcgccagcag agtcggattc gtgttctcca
    10501 cgaagcacgc gttgccggcg agcagtccag tatgaatctg aaatgggaac ttgtgtgcgt
    10561 cggcgaggcg gaccacctgg tggaacatgt ggtcttcgag tgcccgaaac ggccgcactt
    10621 tgtgcgcccg gaagcccgcc ggcgtttcgg cgccgccctt cagcagcctc tcgaactcga
    10681 ggtccgcgtc gtggtcgggc acctcctcga acttgaggtc cctacggtag gcgagcgtcg
    10741 atttcaccga taccatgccc acccggatgg cctgttcgaa ggtcgctgca agcgcccgct
    10801 tgtgatcggc gagcgaatgg atggacacgc cggcgatgcc ctctagccgt tcgatgtctt
    10861 tgcgggacgc cggcgtcacg aatccgtcga atttctgcgc cagcaggaag taatcctggt
    10921 cgaggcgcgc gggcttttcg ttccagtagg ggtccaccag gcaccagtcg atcttcgcgc
    10981 gttcccgcag gacctcacga tagagtccag gtttgttccg gttccggatc acctcgttga
    11041 tccggctgat cgtgccgtgg gagatctcgt ccacgccgta gatgtctttg atggcgattg
    11101 ccagcgcctg tccgtagccg gtgaaccggg ccgcgttcca atacggctcg aaggccgccc
    11161 accgtcgctc cgccggtgct tgttcgtccc gaacagttgc cagcgcgtcc ccggcgagtc
    11221 cggccgagat cacgtcgttc accgcatagt gaccggcgag cccgaagaaa tccacggcct
    11281 gttccaggcg agttttctcc gggatgaggt gctcgtgcga gttgtacagc cgcaactcgt
    11341 ccagcccgcc ggcgagacgt ctggcgagat ccgagctcga tgccgctgaa ggtgcagcgg
    11401 cggcggctgt gcctgggagt acggccgcgt gtcctagaaa ccaccgtctg ttgagtcgca
    11461 tgccttcgaa caaacctcca acgccagcaa agatgcgtcg tcagccaggg cgcctggccc
    11521 gtgccacctt actacttcct cgatgattcc gttgatgctc tctgcaagtg gtgcagtcct
    11581 gccgctgtcg agcgctttaa gcagccgttc gttgccgaaa tcatcgcccg cggcgttttc
    11641 actctcgaca atcccgtccg tatagaagta gagtcggtct ccgacgccaa gccggatcac
    11701 ctgatcctca tacctaacgc cggggaacag gccggccggt agacctcccg cttcgagcaa
    11761 tgtcggctca ccgtctcggg gcacgtagac cggccccgtg tggcccgcca gcacgtaccg
    11821 gaactcgtca gttcgtctgt ccagcactcc atacaacatc gtgaagaact gggggcttcg
    11881 agggtcgagt ggaaatcgag cgttcagttt ctccacgacc tgcgaaggcg tcgaaattac
    11941 aggccggtcc gcgtcgtaca acagcgattg acccggcagc ggcagcagga agtggctgag
    12001 cgtgaccgac aaaagcgcag ctgcgacccc gtggcccgac acgtcgagaa tgtagaggcc
    12061 gacgtcgtga tcgtccagca ggaatacgtt cagcgagtct ccggccagtt cctggcaagg
    12121 ctggtacagc caggcgagat tgatgccgtg aaccttcggc agggatgcgg gcaatagtga
    12181 ctgctgcacc ttcgccgccg cctccaaatc gcgcctcatc gcccggtttg cggcttccag
    12241 cgcgtctgcg gcgtttttct ggtcggtcac gtcggattgc acaccgatga agtgtgtgac
    12301 ctcgccgtcg ctgccgcgaa ctggagtgat cgacaggcgg ttccagaatg gtgttccgtc
    12361 cctgcggtag ttcaggagct gaaccgtgat ctcgcgcttc tcgcgaatgg ccgcgcggag
    12421 ccgttccagc gtctcgtggt ctgtgccgac cccttggagg aaacggcagt tgcggccgat
    12481 aacatcggtc gccccgtatc ccgtgagccg ctcgaatcct tcattcgcgt agatcagagg
    12541 gttatcggga cgccgagcat cggcgatggt gatgccttct gccgtcgcgt cgagcgcacg
    12601 gttcttcaag gcgagctgtt gccggaactg tttccgttcg agactgtctg ctttggctga
    12661 ccgctttgct ctcgcgccgt tcatgggggc tttccagagc atacactgaa tgcatggggg
    12721 tgcggctcgc tgttcttggt tttcttgctg ttttcgtgct ctgggcgcaa gttccggtgc
    12781 tccgcctgac gcagctcgcg gccggcctga caaatcctac cgacatccag cacgccggcg
    12841 acggaacgaa tcgtctgttt gtcgtgcagc agaatgggat tatccgcatc ctctcgggtg
    12901 gcgtgctgcg agcggcaccg tttctcgaca tccgcaatcg caccgtcggg agcggcgaac
    12961 gggggttgct cggtcttgca tttcctccgg gctaccccgc gaagcgctac ttctatgtca
    13021 actacaccaa tctcgccggc gacaccgtga tcgcgcgcta ccgcaccacg gccgatccga
    13081 acgttgcgga ctccaccagc gagaccatcc tactgacgat cgcgcagccg tacgcgaatc
    13141 acaacggcgg gcagctccgc tttggtccgg acggcttcct atacgtcggc atgggtgacg
    13201 gcggaagcgg cggcgacccc cagaacaatg gacagaacct acgcacgctt ctcggcaaga
    13261 tgctccggct cgacgtcgag tccgacttgg cgcgtgtcct gattcctccg gacaacccgt
    13321 ttgtgaacga caccgccgcc cgcccggaga tctgggcgat tggtctgagg aatccgtggc
    13381 gtttctcctt cgaccgagca aaaggcgacc tctggatcgc ggacgtcggg cagaaccgcg
    13441 ccgaagagat tgacttccag tcggcatcca gcctgggtgg cgagaactac ggttggaatc
    13501 agatggaggg gatgcagtgc taccgctcgg gctgtaacac cgcgggcctc atcctgccgg
    13561 tggtcgaata tgtccgctcg gagggctgct cggtgaccgg aggattcgtc taccgcggac
    13621 gcgaatctct cgggcttcgc ggcacgtacc tctacggtga cttctgcacc ggccgtatct
    13681 ggggcctgcg gcgtgagggt gacctgtgga gcaaccgctt gctgctggcg agcgggttgt
    13741 ccatctccac cttcggcgag gacgaggcgg gggagattct cgttgcggat tacggcggcg
    13801 ctatctaccg gatcggcggt cccgttgccc cgttcttcac cagcgcggga gtcaccaatg
    13861 ccgcgagcta cgctagcggg ctcgttgccg gctcactcgc tacgatcttt gccgccggcg
    13921 tgaaggacga taacgga
//
LOCUS       DATIFQ010000318         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2022088, whole genome shotgun sequence.
ACCESSION   DATIFQ010000318 DATIFQ010000000
VERSION     DATIFQ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022088"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..617
                     /locus_tag="VLE22_14925"
     CDS             90..617
                     /locus_tag="VLE22_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15750.1"
                     /translation="MKGHRLVAAVGILGLVLLGGVASSASASGKESFSFNAPVISGFP
                     GGRAVELTGGGAYILPDFVHAGGSCGCTSDITAGLFTGCQNGQGVRWDTDTVLPSTTF
                     KCTGAASEALKAAETGDKTVVMLADFYRAGDGNDESFTAQMIVSDTDLDPVADGVQNI
                     WIQQVGCGTALVNFN"
     gene            682..2067
                     /locus_tag="VLE22_14930"
     CDS             682..2067
                     /locus_tag="VLE22_14930"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSB15751.1"
                     /translation="MAQLQAAKIEEFKSGFNGEVLLPGNDAYESARKIWNAMIDKRPA
                     LIARSATTTDVVRAVNFARDNGLLLAVRGGGHNIAGNAVCDGGLMIDLSKMKAARVDP
                     ARRRVTIEGGATLADLDAATQAHGLATPVGINSTTGVAGLTLGGGFGWLSRKYGMTVD
                     NLESAEVVTAAGEVVRASATEHLDLFWALRGGGGNFGVVTRFEFRLHPVGPDVLSGLI
                     VYPISEAKSVLQQYRDFIARAPEELSVWTVLRQAPPLPFLPEKVHGKEIIALALIYAG
                     DPKQGEPLIEPLRRFGTPLGEHVGAQPYVAWQQTFDPLLTPGARNYWKSHNLSTIKDA
                     LIDTIIAYTLKLPSPQTEIFLGAIGGATTRPAPDAMAYAHRDAKFVMNVHGRWEEPAD
                     DKRVIAWARDFFNACAPFASGGVYVNFLTADEVDRVRSAYGPNYDRLAQVKRKYDPKN
                     LFRVNQNIVPA"
BASE COUNT          417 a          712 c          685 g          374 t
ORIGIN      
        1 ttctcggacc aaccaacgtg aatcgagaaa gaaagcacta acaggcaaga accgggcgtg
       61 acatttcgtc cggtcacaaa ggagacggca tgaagggaca tcgtttggta gctgccgtgg
      121 ggatcctggg actcgtgctt ctgggaggag tggcgtcgtc ggcgtcggcc tcggggaaag
      181 agtcgttcag tttcaacgct cccgtcattt ccgggttccc gggaggccgg gcggtggaac
      241 tcaccggcgg cggtgcgtac atcctgccgg atttcgttca cgcgggtggc agctgtggtt
      301 gcacgtcgga tatcaccgcg gggctgttca ccggatgcca gaacggacag ggagtgcggt
      361 gggacacgga cacggtgctg ccatccacaa ccttcaagtg caccggcgcc gccagtgaag
      421 ccctcaaagc ggcggagaca ggagacaaga ccgttgtgat gctcgcggac ttctaccggg
      481 cgggggatgg caacgacgaa tcgttcaccg cccaaatgat cgtctcggac accgatctcg
      541 atcctgtcgc tgacggggtc cagaacatct ggatccagca agtcggctgc gggacggcgc
      601 tcgtcaactt caactgaggt ccggtcgagg aacgcctctt tcgaaacgtg gaagaatcca
      661 ggccacaccg aggaggagat catggcgcaa ttgcaggcag cgaaaatcga ggagttcaag
      721 agcggcttca acggagaggt ccttcttccg ggaaacgatg cctatgaaag cgcgcggaag
      781 atctggaatg cgatgatcga caagcgtccc gcactcattg ctcgctccgc cacgaccacg
      841 gacgtggtcc gagccgtgaa ctttgcaaga gacaacggac tcctgctcgc cgtccgcggt
      901 ggcggtcaca acatcgcggg caatgcggtg tgcgacggcg ggctcatgat cgacctttcg
      961 aagatgaagg ccgcgcgggt cgatcccgcc cggcgccgcg tcacgatcga gggtggcgcc
     1021 acactcgcgg acctcgacgc cgccacccag gcgcacggcc tcgccacgcc ggtcggaatc
     1081 aactcgacga ccggcgtggc gggactgacg ctgggcgggg gcttcggatg gctcagccgg
     1141 aagtacggca tgaccgtcga caacctcgag tccgccgagg tggtgaccgc ggcaggtgag
     1201 gtggtgcgcg cgagcgccac cgagcacctc gatctcttct gggctcttcg cggcggtggc
     1261 ggcaatttcg gcgtcgtcac ccgcttcgaa ttccggctcc atcccgtggg tccggacgtg
     1321 ctctccgggc tcatcgtcta cccgatctcc gaagcgaaat cggtgctgca gcagtatcgt
     1381 gatttcatcg cccgagctcc cgaggagctg tcggtctgga cggtgctgcg ccaggcgccg
     1441 cccctgccgt ttctgcccga gaaggttcac ggcaaggaga tcatcgcgct cgctctgatc
     1501 tatgcgggtg acccgaagca gggtgagccg ctcatcgagc cgttgcgcag gttcggcacc
     1561 ccgttgggcg agcacgtcgg cgcccagcca tacgttgcct ggcaacagac cttcgaccct
     1621 ctcctcaccc ccggcgcccg caactactgg aagtctcaca atctctcaac gatcaaagac
     1681 gcgctcatcg acaccatcat cgcgtacacc ctgaaactgc cgtcacctca gaccgagatc
     1741 tttctcggcg ccatcggcgg tgccaccaca cggccggccc cggacgccat ggcctacgcc
     1801 catcgcgacg ccaaattcgt catgaacgtc cacggccgct gggaggagcc ggccgacgac
     1861 aagcgcgtca tcgcctgggc gcgcgacttc ttcaatgctt gcgccccctt cgccagcggt
     1921 ggcgtctacg tcaacttcct caccgccgac gaggtcgacc gcgtgcgctc cgcgtacggc
     1981 ccgaattacg atcgcctcgc ccaggtgaag cgcaagtacg acccgaaaaa cctcttccgc
     2041 gtgaaccaga acatcgtgcc ggcctgaaaa acgagatacg cgtgactagc gaaggcatcg
     2101 cgagacgcgt cgaagccggt gagccccgtg ccgcctgaac cgccggtctc cttgaggccc
     2161 ggcttgatta ctgtccttcc cttcctcg
//
LOCUS       DATIFQ010000319         3915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1829344, whole genome shotgun sequence.
ACCESSION   DATIFQ010000319 DATIFQ010000000
VERSION     DATIFQ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829344"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VLE22_14935"
     CDS             complement(<1..541)
                     /locus_tag="VLE22_14935"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HSB15752.1"
                     /translation="MSDSRRIAVLGAGNIGAALIGGILKGGVADRKHVVATVRTAGRA
                     REVSEKYSIPVTAGGNREVAEWSDILIVAVKPPTLPRLLEEIRGVLRSDQILISLAAA
                     VPISLIEKTVDKRIPVFRAMPNIPVVVEEGATAVSANAAVTASHRKIVESIFRAVGVV
                     CFVEEDQMHAVTALSGSGPA"
     gene            complement(605..1546)
                     /locus_tag="VLE22_14940"
     CDS             complement(605..1546)
                     /locus_tag="VLE22_14940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB15753.1"
                     /translation="MADYSRRDLVRLVAAGSAFVRPSLAAGSIPTRRLGKISFQAGIL
                     GLGAQHLGDFDVGQSVVDRVIGEALDNGLNYIDTAPPYNLSEERVGRALKGKRDKVFL
                     VSKVETNAKGDALYQVRDSLRKLQTDHLDCVHIHNISRDDRYPSLDQVISPNGTLGAL
                     IEAKKQGMIRHIGCTSHLRPGRVLPAFETGEIELFMCTVNFVERHIYGYEEKVLPEAR
                     RRNIGVIAMKVLGGPVPGPGARLVSPEDYQTTLRYVWGVSGVSVAIIGLRTPEELRQA
                     LASARSFKPFDQAEMAAVTDRGKLLAAQWGPLRGPVA"
     gene            1682..3751
                     /locus_tag="VLE22_14945"
     CDS             1682..3751
                     /locus_tag="VLE22_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB15754.1"
                     /translation="MNRPRKRVPMAVLSVLAFVAPAADILAQAPTDGAAAKGVSRADA
                     YYHFSMGHLYTELAGAYGNRGTYLSKAIDHLKLAIQADPSSSYLAERLSDAYVQAGRL
                     NDAVTEAEDRLKKNPDAIEARRVLGRIYARLIGDTQNNRINEEMLRKATEQYQKIVAK
                     EPGDFDSWLMLGRLHKVAQNSIESEKAFSRAIEIDPTSEEAMTGLAMVYSDLGDSKRA
                     MEMLDRVARQNPNLRTLTTLANAHEQMRDYASAAQVLRQALEMAPENIELKRALAQNL
                     LFSGQEDEALRLYQDIAAAEPKDAQTHLRVSQIYRQKRDFVKAREASERARQADPDNL
                     EVRYNEVNLLEAEGRMEEAIAKMKELVDSGEKKSSSQAERGNRAVLLERLGLLYRSGE
                     QYKQAVETFRKMEEINPESASRAAAQVMDTWRQAKEYSKAIEEADAAYKRFPKDRTIL
                     LIRASLLADMGRGDEAVSEVKKLLDGKSDREIYLALAQTYEKTKNYGEMGKALDAAEK
                     LSMSSEDKQTVYFMRGAMYERAKNFDAAEAEFRKVLEVDPENASALNYLGYMLADRNV
                     RLEEALRSIDKAVKLDPNNGAYLDSLGWVYYRLDRLDEAEKHLRRALERVSRDPTVRD
                     HLGDVLAKKGQLKDAIGQWEISVKEWEASAPSEKDPAEIAKITKKLEGARIRLAKESS
                     STIAKPE"
BASE COUNT          836 a         1115 c         1297 g          667 t
ORIGIN      
        1 aagccggtcc gctgccggag agcgccgtga ccgcgtgcat ctggtcctct tcgacgaagc
       61 acaccacgcc gacggcgcga aagatgcttt cgacgatctt gcggtgggat gcggtcaccg
      121 cggcgttcgc cgagacggca gtggcccctt cctcaaccac gaccgggata ttcggcatcg
      181 cgcgaaagac ggggatgcgc ttgtctacgg ttttttcaat cagagaaatg ggaacggcgg
      241 cggcgagtga gatcaggatc tggtcgctgc ggagcactcc cctgatttcc tcgagcaggc
      301 gcggcagcgt tggcggcttc actgccacga tcaggatgtc cgaccattcg gccacttccc
      361 ggttgccgcc ggcggtcacc gggatcgaat acttctcgga gacctcacgg gcgcggccgg
      421 cggtacggac ggtggccacc acgtgcttgc ggtccgcaac acctcccttg agaatgccgc
      481 cgatcagcgc cgcgccgatg tttcccgcac cgagcacggc gatccgcctg gaatcggaca
      541 tgccgacatt gtatacggtt gtccggacag gcgggtttgc ggagcgaatg gtgctcgatg
      601 ttggtcaggc gacgggaccg cgaagggggc cccactgtgc ggccagcaac ttaccgcggt
      661 ccgtgacagc ggccatctcg gcctgatcga atggtttgaa cgaccgggcg gaggcgagag
      721 cctgacgaag ttcctccgga gtacgcaggc cgataatcgc gacagaaacg ccagaaacgc
      781 cccaaacgta acgcaacgtg gtctggtaat cctccggcga gaccaagcga gcgcccggcc
      841 cagggaccgg gccgccaagt accttcatgg caataacacc gatattccgg cgccgggctt
      901 ccggaaggac cttctcctcg taaccgtaga tgtgacgctc gacaaagttg accgtacaca
      961 taaacagctc aatctccccg gtctcaaacg cggggagcac gcggccggga cgaaggtggc
     1021 tggtgcagcc gatgtgccgg atcatcccct gtttcttcgc ctcgatgagg gcgccgaggg
     1081 tgccattcgg gctgatgacc tggtccaggc tggggtagcg gtcgtcgcgg ctgatgttgt
     1141 ggatgtggac gcagtccaga tggtcggtct gcagttttct gaggctgtcc cggacctggt
     1201 agagcgcgtc ccccttggcg ttggtctcca ctttggagac gagaaacacc ttgtcgcgct
     1261 tccccttgag cgcgcggcca acccgctcct cggagaggtt gtaaggcggc gcggtatcga
     1321 tgtagtttag tccgttgtcg agtgcttcgc cgatcacgcg gtctacgacg ctctgtccaa
     1381 cgtcgaagtc accgaggtgc tgggcgccga gaccgaggat gccagcctga aaactgatct
     1441 tgccgaggcg gcgggtagga atggagccgg cagccaagga ggggcgcacg aatgcggatc
     1501 cggcagctac gaggcgaaca agatccctgc gggaatagtc ggccatggga tgtattctac
     1561 acgggacgtc gcgcttaagt cccaagccaa gccataaccc tgtgaactga ggatatactg
     1621 ttggaagaga ggacacttgg ccgctggtcc gtttcctggg tagcggtaag aggctcgttc
     1681 catgaatcga ccgagaaaaa gagttcccat ggccgtgttg tctgtactgg cgttcgtggc
     1741 gccagcggcc gacattctgg cccaggctcc aacagatggg gccgccgcga agggcgtgtc
     1801 gcgtgcggac gcctactacc acttctcgat gggccacctc tataccgaac tggctggcgc
     1861 gtacgggaac cgcggcacgt atctctcgaa ggccatcgac cacctcaagt tggcgattca
     1921 ggccgatcca tcgagcagtt acctagccga acgactatcc gacgcctatg tccaggcggg
     1981 gcgcctcaac gacgcggtga cggaagcaga ggaccggctg aagaagaacc ctgacgcgat
     2041 cgaggcccgg cgggtgcttg gacggatcta cgcccggctc atcggcgaca cgcagaataa
     2101 ccggatcaac gaggaaatgc tccggaaggc aacggagcag taccaaaaga tcgtcgcgaa
     2161 agagcctggc gattttgaca gctggctgat gctgggccgc ctacacaagg tggcacagaa
     2221 ctcgattgag tcggagaagg cgttcagccg cgcgattgag atcgacccga cgagcgaaga
     2281 agccatgacg ggtctcgcta tggtctactc cgatctcggc gacagcaagc gggcgatgga
     2341 gatgctggac cgggtcgccc gccagaatcc gaaccttcgg accctgacga cgctggccaa
     2401 cgcccacgag cagatgcgcg actacgcgag cgccgcgcaa gtgctccgac aggcgctgga
     2461 gatggcgccc gagaacatcg agctgaagcg tgcgctagca cagaatctcc tcttttcagg
     2521 ccaagaggac gaggcgcttc gactctacca ggacatcgcc gcggcggagc cgaaggacgc
     2581 ccagacgcac ctgcgggtct cacagattta ccggcagaag cgcgactttg tgaaggcgcg
     2641 ggaggcgtcg gagcgggcgc gccaggccga tccggacaat ctggaggtcc gctacaacga
     2701 ggtgaacctg ctggaagccg aaggcagaat ggaggaggcc atcgccaaaa tgaaagagct
     2761 ggtcgactcc ggggagaaga agtcctccag ccaggcggag cgtgggaatc gtgcggtgct
     2821 gctggagcgg cttggtctgc tctaccgatc gggcgagcag tacaagcaag ccgttgagac
     2881 cttccggaag atggaagaga ttaacccgga gtcggcgtct cgtgccgcgg cgcaggtgat
     2941 ggacacctgg cggcaggcga aggagtattc caaagcgatt gaagaagccg atgccgctta
     3001 caagaggttc ccgaaagaca ggacgatcct tctcattcgt gcctctttgc tggcggacat
     3061 gggccgggga gacgaggcag tctccgaagt gaagaagctg cttgacggca agagcgatcg
     3121 cgagatttat ctggcgctgg cacagacata cgaaaaaacg aagaactacg gcgagatggg
     3181 aaaggcgctc gacgccgcgg agaaactgtc catgtcgtcg gaggacaaac agactgtcta
     3241 tttcatgcgc ggcgcaatgt acgagcgcgc gaaaaacttc gacgcggcgg aggccgagtt
     3301 ccgcaaggtg cttgaagtcg atcctgagaa tgcctcggcg ctgaactacc tgggttacat
     3361 gctggcggac cgcaacgtcc ggttggagga ggcactccgt tccatcgaca aggcggtgaa
     3421 actggacccc aacaatggcg catacctgga cagcctgggc tgggtctatt accggctgga
     3481 caggctcgac gaggccgaga agcatctgcg ccgggcgctg gagcgggtct cacgcgaccc
     3541 aacggtgcgt gaccacctcg gagatgtgct agcgaagaag ggtcaactga aggatgcgat
     3601 cgggcagtgg gagatttcgg tgaaggaatg ggaagccagc gccccgtctg agaaggatcc
     3661 cgcggagatt gcgaagataa cgaagaaact cgagggggcg cggattcgtc tggcgaagga
     3721 atcatcgagc accatcgcca agccggaata gaccttttcg catttcggca gttcagcgcg
     3781 ataatagtga aagcggcgag gtggctggtg cgtttcacgt tcccattgtg gctggtgttt
     3841 cttgcgattc cccttgccgc tcagcatgga tcaagagccg cggagatcga agctgcgcgc
     3901 gatgaaaagg cgaag
//
LOCUS       DATIFQ010000320         5154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_871420, whole genome shotgun sequence.
ACCESSION   DATIFQ010000320 DATIFQ010000000
VERSION     DATIFQ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871420"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VLE22_14950"
     CDS             <1..782
                     /locus_tag="VLE22_14950"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HSB15755.1"
                     /translation="SFHQDARLLFEYRYSAEAPKTYVHPFCAPDGNVTLDSPADHVHH
                     RGVMLAWSDVNGFDFWGEQNPGKHGRIVHRQFEELRKARLVSLEDWVAEGKTLLTERR
                     TIAVPISSKDAVWLDWTSELSAVSGPVQLSAAEHVYNGLGIRFAHELDDGNVLNSKGT
                     TGIEKTNGDDADWCAYSAAVRNRGLAGTVIFNHPSNPRHPIPFFVMNKPFGYISAAPT
                     FREPFRLEMGRPLRLRFAVVGFNGEPSVERMNGMFRQWVRG"
     gene            complement(823..1845)
                     /locus_tag="VLE22_14955"
     CDS             complement(823..1845)
                     /locus_tag="VLE22_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB15756.1"
                     /translation="MNGYERIAYALRGEQPDRVPVMLHNFMMAAREAGYTMRQYRESP
                     EAIAHSYVAAVEKYGYDGILVEIDTSVLAGSLGVPVDFPEDMPARCVRRRLERLEDVA
                     GLPPADVGRYWQVQVWLEAVRLLKRQFGNEFFLRGNCDQLPFSLASAMRSMEGWMLDI
                     SEPEKRTLVERLLDYCTEATSQFIRLMARAGAHMVSNGDSPAGPDMVSPRMYCEFALP
                     YERKVVECAHACGLPYLLHICGKTDRILEAMLSTGADGLELDYKTSSQLAHDVMKDRA
                     VFVGNIDPSGVLALGTPQLVEEKTRALLRIFADTPRFILNAGCAIPADTPPENLHAMI
                     RAARES"
     gene            complement(1842..2480)
                     /locus_tag="VLE22_14960"
                     /pseudo
     CDS             complement(1842..2480)
                     /locus_tag="VLE22_14960"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
     gene            complement(2477..3385)
                     /locus_tag="VLE22_14965"
     CDS             complement(2477..3385)
                     /locus_tag="VLE22_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15757.1"
                     /translation="MRPCVLFALLSAVLSVRAQVDTGKILARVAEEAEAFRRLAPDVL
                     GEETLKQRARKGASRFRPRIGSGGEKLSAPRYATKEIVSEYGYSAFSEAPDSLHEFRE
                     VVSVDGRAVKAREKARQTLAEGMRSQDDRLKKRLLEEFERHGLTGAVSDFGQLILLFG
                     QRRLEKYEFQFAGKARIGPDEAIALSFRQTSDQGGVTIFENRKVVRRPLQGELWVRET
                     DFLPLRIKLVTVREHDRYVIRDEATVDYAMTGHGALMPASVVHRQYAADQLMVENLFQ
                     YGTFRKFSAETEVKFTESPAQPKDKK"
     gene            complement(3391..4485)
                     /locus_tag="VLE22_14970"
     CDS             complement(3391..4485)
                     /locus_tag="VLE22_14970"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15758.1"
                     /translation="MADKVKVGIIGSQFEADIHAASFQLMPEEAEVVAVSSPTAGHPE
                     ELARRYSIPRVFRDYREMLMEKDIEMVTIAAPNSLHAQMTADIARAGKHVVCEKPLCM
                     TLEEADEMIDVCRKKGVLLLYAEELFFTPKYVKAKEMADQGAFGRVYLVKQSEKHFGP
                     HAPWFWDVERSGGGVFMDMGCHGIAFCYWLLGRPAIKSVFCNMGTYVHSSKTKGEDDC
                     ISVIEFENGAVGLVEDSWARRGGMDDRIEVYGEGGVTYANLHMGNALPTYSEYGYGYA
                     VEKAPTTKGWTYPVFEELWNYGFPQEMRHFARCVRGKETPQATGEDGRVVMEALYAGY
                     ASAGEGRKVSLPFRPSGARKPIDLWLNRRS"
     gene            4568..>5154
                     /locus_tag="VLE22_14975"
     CDS             4568..>5154
                     /locus_tag="VLE22_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15759.1"
                     /translation="MTSRAFLAFFCFTTFLAAQPSRPDINYDESKVPKYTLPDPLVMS
                     DGTPVKDAKAWTAKRRPEILRLFEVEMFGRSHGKLKDTSYEPLSIDREALGGKAVRKE
                     VAVLFTGKKDGPRMDILIYLPADAKDPVPLFVGLNFAGNQAVRAEPGIRLSTSWMRDS
                     RDDSVVNHRATEKSRGAAASRWAVEKILARGYGLAT"
BASE COUNT          973 a         1603 c         1510 g         1068 t
ORIGIN      
        1 tctcattcca ccaggacgct cggcttcttt tcgagtaccg atattccgca gaagccccaa
       61 agacctatgt tcacccgttc tgcgcgccag acgggaacgt caccctcgac agcccggcgg
      121 accacgtgca ccatcgcggg gtgatgctgg cctggagcga cgtcaacggc ttcgattttt
      181 ggggagagca gaatccaggc aagcatggcc gaatcgtcca ccggcagttc gaggaactcc
      241 gcaaggccag gcttgtctcg ctcgaagact gggtcgccga aggcaagacg ctcctcaccg
      301 agcggcgcac cattgccgtg cccatctctt ccaaagatgc agtgtggctc gactggacga
      361 gcgagctcag cgccgtttcc ggaccggtcc agctctcggc ggcggagcac gtctacaacg
      421 ggctgggaat ccggttcgca cacgagctgg atgacggcaa cgttctgaac tcgaagggaa
      481 ccaccgggat cgagaagacg aatggcgatg acgcagactg gtgcgcctac tcggcggcgg
      541 ttcggaatcg cggcttagcg ggaacagtaa tctttaacca tccctccaac ccccgccatc
      601 cgatcccgtt tttcgtgatg aacaagccgt tcggctatat tagcgctgcc ccgacgtttc
      661 gtgagccttt ccggctggag atgggccgtc cgctgcgtct acggttcgcg gtggtgggtt
      721 tcaacgggga accgagcgtg gagcgaatga acggtatgtt tcggcagtgg gtccgcgggt
      781 agctcccggg gattgtcggt gggcgacgtc gactaagcgc gatcaggact cacgtgccgc
      841 gcggatcatg gcgtggagat tctcgggcgg ggtgtccgcc ggaatcgcgc acccggcgtt
      901 gagaatgaag cgcggggtat cggcgaagat tcgcagcaac gcgcgtgttt tttcttcgac
      961 aagttgtgga gttccgagag cgagcacgcc gctcggatcg atatttccca caaatacggc
     1021 gcgatccttc atgacatcgt gcgccagttg ggagcttgtc ttgtagtcaa gttccagccc
     1081 gtcggctccc gtcgagagca tggcctcgag aatcctgtcg gtcttgccgc agatgtgcag
     1141 gagatacgga agcccacacg cgtgggcgca ctcgacgacc tttctctcgt acggaagcgc
     1201 gaattcgcaa tacatccgcg gcgaaaccat gtccggacca gcagggctgt cgccgttgga
     1261 gaccatgtgc gccccggctc tagccatcag tctgatgaac tggctcgtcg cctccgtgca
     1321 gtaatcgagc agtcgttcga caagcgtccg tttttccggc tcggagatat caagcatcca
     1381 cccctccatg gaccgcatgg ccgaggcgag agagaaaggt agctggtcgc aattgccgcg
     1441 caggaagaat tcgttgccaa actggcgctt gagcagccgc acggcctcca gccacacctg
     1501 gacctgccag tatcgcccga cgtccgccgg cggcagccct gcaacgtcct ccaaccgttc
     1561 cagtcgcctc ctgacgcacc tcgccggcat gtcctccggg aagtccaccg gcacgccgag
     1621 tgatccggcc aggacggacg tgtcaatctc gacgagtatg ccgtcgtatc cgtacttttc
     1681 tacggccgcc acataggaat gcgcgattgc ctcggggctt tcacggtact gccgcatggt
     1741 gtagccggcc tctcgggccg ccatcatgaa gttgtggagc atcaccggca ctctgtcggg
     1801 ctgctctccg cgcagagcgt acgcgattcg ctcgtaaccg ttcatttcgc ctcctccaaa
     1861 aggaagcccc gcacccctgc ccacatcgcg tccacgacat ccgcgagttc gtgatccgac
     1921 tccaatagct ccagcctgac atttggccgg gacttcgcga actcgatgga gtactcgtgc
     1981 ggcaccaagg tatcgttccg gccgtggtac accagggccg gctgcgtgac gtccggatag
     2041 ttttcgtact gcaagccgtc cgcgatcagt ccataatgaa cgcggcgttg tctttgttcg
     2101 gcgtagtgat acaaggaaag ccacccagtt cgcttccatt cctcgagttg cgagggttcg
     2161 agcgtttccg tccagcgtcg cgcaaatccg aaagccggcg ccagcaggat gacccggcgt
     2221 acctccggat gacgtgccgc gtaaagctac gcgaggtagc ctcccatact cgacccgatc
     2281 agcactgccg gccctgctgc agcgaggcgc tcaatcacac gaagctggcc ggagatggtg
     2341 aggtgctcga agtctccttc tgcgagatcg ggcacctgga ttcttaaccc ggccttgtcg
     2401 aatcgttcct ggaagtgcct ggccttcttc gaggagggcc ccgacgcaaa cccgtgaaga
     2461 tagatgatcc tttcgttcac tttttgtcct ttggctgcgc tggtgattct gtgaacttca
     2521 cttcagtctc ggccgagaac ttgcgaaacg tgccgtattg gaacagattc tccaccatga
     2581 gctgatcggc ggcgtactgg cggtgcacga cgctggcggg catgagcgca ccatggccgg
     2641 tcattgcgta gtctacggtc gcttcgtcgc ggattacgta cctgtcgtgc tcacgcaccg
     2701 tgaccagctt gattcgtagc ggtaggaagt ctgtttctcg tacccacaac tcgccctgaa
     2761 gcggtctgcg caccaccttg cggttctcaa agatcgtgac tcctccctgg tcactcgtct
     2821 ggcggaagga aagcgcaatc gcctcgtctg ggccgattct agctttgcct gcgaactgga
     2881 attcgtattt ctcaagcctc cgctgtccaa acagcaggat caattgtcca aaatcggaga
     2941 cagccccggt gagcccgtgc cgctcgaatt cttcgaggag gcgcttcttg agccggtcgt
     3001 cctgagatct catcccttcg gcgagcgttt ggcgcgcttt ttcgcgagct ttgaccgcac
     3061 gcccgtcgac ggagacaact tcccgaaact cgtgcagaga atccggcgct tcgctgaagg
     3121 ccgagtagcc gtactcggac acgatctctt tcgtcgcgta cctcggggct gaaagctttt
     3181 cgccgccgct tccgatgcgg ggacgaaatc gagacgctcc cttccgggcg cgctgtttca
     3241 gcgtctcttc gccgagaaca tccggcgcga gccttcggaa tgcttccgct tcttcggcca
     3301 cccgggcgag aatcttgccg gtgtccactt gcgcgcgtac ggatagaacg gctgacagta
     3361 acgcgaagag cacgcaaggg cgcattgttg ttagctccgc cggtttagcc agagatcgat
     3421 cggctttctg gcgcctgacg gccggaacgg caggctcacc ttacggccct caccggcgga
     3481 ggcgtacccg gcgtacagag cttccatgac cacgcgcccg tcttctccgg tcgcctgcgg
     3541 cgtctccttg ccgcggacgc agcgcgcgaa atgccgcatc tcctgcggga aaccgtagtt
     3601 ccacagttcc tcaaagacag ggtacgtcca gcccttggtc gtcggagcct tctcgaccgc
     3661 gtatccatag ccgtactcgc tgtaggtggg cagtgcattg cccatatgca ggttggcgta
     3721 ggtgacgccg ccctcgccat agacctcgat gcggtcgtcc attccgccgc ggcgcgccca
     3781 actgtcctcg acgaggccga cggcgccgtt ctcaaattcg atcacggaga tgcagtcgtc
     3841 ttcgcccttc gtcttgctcg aatgcacgta ggtgcccata ttgcaaaaga cgctcttgat
     3901 cgccggacgc ccgagcagcc aatagcaaaa cgcgatgccg tggcacccca tgtccatgaa
     3961 gacgccgccg ccagagcgtt cgacgtccca gaaccacggg gcgtggggac cgaaatgttt
     4021 ctcgctttgt ttgaccagat agacccggcc gaaggcgccc tggtcggcca tctccttcgc
     4081 cttgacgtat ttcggcgtga agaaaagttc ttcggcgtac agaagcagca cgcccttctt
     4141 acggcagacg tcgatcattt cgtccgcttc ttcgagcgtc atgcacagcg gcttttcaca
     4201 gaccacgtgc ttgcccgcgc gggcaatgtc ggcggtcatt tgcgcgtgca aggagttcgg
     4261 tgctgcgatg gtgaccatct cgatgtcttt ttccatcagc atctcgcgat agtcccggaa
     4321 aacgcgtggg atgctgtagc gccgtgccag ctcttccgga tgcccggccg taggtgacga
     4381 gacggccacc acttcagctt cttccggcat cagttggaac gatgccgcgt gaatgtccgc
     4441 ttcgaactgc gaaccaatga ttcctacctt gaccttatcc gccatgtctc ctcactctgt
     4501 acaacatcca cacggattac atcacagctt caggtgcggc gctgggattt cgtgtaatct
     4561 ggctgacatg acatcgcggg cattccttgc attcttctgc ttcacgacat tcctggccgc
     4621 gcaacccagc cggcccgaca tcaattacga cgaatccaag gttccgaaat acacgcttcc
     4681 cgatccgctg gtgatgtcgg acggcacacc ggtcaaggat gcaaaagcct ggacagcgaa
     4741 acgccggccg gagattctga ggctgttcga ggtggaaatg ttcggccgca gccacggaaa
     4801 gctgaaggac acttcctatg aacctctgtc gattgaccgg gaagctctgg gcggaaaggc
     4861 ggttcggaaa gaagtcgcgg tgctctttac ggggaagaaa gacgggccgc gcatggacat
     4921 cctgatctac ctccctgcgg atgcgaaaga ccctgtgccg ctgttcgtcg gtctgaattt
     4981 cgctggcaat caagcggtgc gagccgaacc tggaattcgg ttatccacga gttggatgcg
     5041 tgacagcaga gacgatagcg tggtgaacca ccgtgccact gagaaatccc ggggtgcggc
     5101 tgcaagccgg tgggctgtcg aaaagatcct cgcccgcggc tatggacttg ccac
//
LOCUS       DATIFQ010000321         2583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1445994, whole genome shotgun sequence.
ACCESSION   DATIFQ010000321 DATIFQ010000000
VERSION     DATIFQ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445994"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VLE22_14980"
     CDS             <1..213
                     /locus_tag="VLE22_14980"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSB15760.1"
                     /translation="AILLPVGALGAWVYWKNGNLNVRYSLLIAAGLLIGAFLGAKLVE
                     PVSDLTLRRIFGGFLLLISIRMILGK"
     gene            complement(220..678)
                     /locus_tag="VLE22_14985"
     CDS             complement(220..678)
                     /locus_tag="VLE22_14985"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HSB15761.1"
                     /translation="MRRATVLAMILIAVPTGRLSAADEAKIRAVNEAYAAAWRRNDPE
                     AVRATFWPDAVLIPQGSPPVRGLEAIDRFWWPKDAPATTVTGFTVTADEVGGSGNLGF
                     VRGSFVLDFSWEDKGKTVSRRNRGNYFMLFRRGAAGDWRISHRMWGDLPR"
     gene            complement(774..2393)
                     /locus_tag="VLE22_14990"
     CDS             complement(774..2393)
                     /locus_tag="VLE22_14990"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HSB15762.1"
                     /translation="MLGNSGAGPPSRLSGLAILSWAIVAFLLLFAATGPWRIVGVEIN
                     RLAVPLGAALGLVILWMAVFPVARSASAGAFLREDLHISRRLIAAGGAAATVLVYLLI
                     RNRYYGFEVNAWDFSFYDRPLAHPFNGGFLFNDIENRSVLGTHAYFLLLPFLPLYAIT
                     PSPQWLLGGQAVLVGLAVVAGFYCLRKVSEDDVTAALLSAAFLVNPYTARAAQYVFHP
                     EIFYPACLFLLLHGFLAKRPVLFVLGLGATAAVKEDAVLPLVGLALSASLCYRRHRWA
                     AVAAGAGVGLFLLDYYVVLPHFAGGPPWYEHYWARYGSSPLAAAVGMATHPVALAADF
                     MRSGFWRLAATLAFTLFVGYETLLAAVPMILSYGAAANTRLSQLRLYYSLPLLPFVFL
                     AAASGLKKLGSLGTKTYAGHARARCRVGALVVFLCSAFFGPGYRLVGRQAAEVPSRLA
                     RSAADQPLLVQGALFPHVGYPRNYSVLKPPVVLDGRHGFLIAPHVSPYPFSRNELQAI
                     AERLLADSNYRVTTDRGVMLAAPATWATPQD"
BASE COUNT          534 a          798 c          836 g          415 t
ORIGIN      
        1 gcgatccttc tgccggttgg agctctcggc gcatgggtct actggaagaa cggcaacctg
       61 aacgtgcgct actcgctcct gatcgccgcg ggccttctca tcggcgcgtt tctcggagcg
      121 aagctcgtcg agcccgtctc cgacctgacg ctgcgaagga tcttcggcgg cttcctgctt
      181 ctcatctcga tcaggatgat cttggggaag taggcagcct cacctcggga ggtcgcccca
      241 catacggtgg gagatcctcc agtcgcccgc ggcgccgcgc cggaagagca tgaagtagtt
      301 gccgcgattg cggcgcgaca cggtctttcc cttgtcctcc caggaaaagt cgagcacgaa
      361 gctcccgcgg acgaatccga gatttccgct ccctcccact tcgtcggccg tcacggtgaa
      421 gccggtgacg gtcgtcgccg gtgcgtcctt cggccaccag aagcggtcga tcgcctcgag
      481 gccccggacg ggcgggctgc cctgtggaat gagcacggcg tccggccaga acgtcgcccg
      541 aaccgcttcg gggtcgttgc ggcgccacgc cgcagcgtag gcctcgttga ctgcgcggat
      601 cttcgcttcg tcggcggcgg agagccgccc ggtcggaacg gcgatgagga tcatcgcgag
      661 aaccgtcgct cgccgcatga gggttcctac gaaagtcgat cccaccggtt ctgtcgccaa
      721 gatctgtttc cgggcggcgc gccggcagcg aatccgggcg aaggttggct ggatcaatct
      781 tgcggggttg cccaggtcgc cggagccgcg agcatcacgc ctcgatcggt cgtcactcga
      841 tagttcgaat cggcaagaag tctctcagcg atcgcttgca gttcgttccg actgaacgga
      901 tagggtgaaa cgtgaggagc gatgagaaag ccgtgcctcc catcgagcac cacgggaggc
      961 ttcagaacgg aatagtttct cgggtaaccg acgtgaggga acaatgcgcc ttgcaccaga
     1021 aggggttggt cagcggctga cctcgctaac cgtgagggaa cttcggcggc ctggcgtccg
     1081 acgagccggt atcccgggcc gaagaaggcg ctgcaaagaa aaacaacgag cgcaccgacc
     1141 cggcaacgcg cgcgtgcgtg cccggcgtag gtctttgtcc ccagtgatcc gagcttcttc
     1201 agtccgctcg ccgctgcgag aaacacgaac ggcagcagtg gaagggagta atacaggcgc
     1261 agttgcgaca accgggtgtt agccgcggcg ccataagaca ggatcatcgg gacagcggcg
     1321 agaagcgtct cgtagccgac aaaaagtgtg aacgcgagag tcgcggcaag tctccaaaac
     1381 cccgaacgca tgaagtcggc cgcgagtgcg acgggatgag tcgccattcc gaccgccgcc
     1441 gccaacggag acgaaccata tcttgcccag taatgctcgt accacggcgg gccccctgcg
     1501 aaatgcggta acaccacgta gtagtcgagc aagaagagtc ctacccctgc tccagctgcc
     1561 accgctgccc accggtgacg tcggtaacag aggctcgcgc tgagggcgag accgacgagc
     1621 ggcaggacgg catcttcctt gacggccgct gttgcgccga ggccgagtac gaaaagcacc
     1681 gggcgcttgg caaggaagcc atgcaacagg agaaagagac acgcaggata gaagatctcg
     1741 ggatggaaga cgtactgtgc tgcccgggcg gtgtacggat tcacgaggaa ggccgcggaa
     1801 agcagtgccg cggtgacgtc atcctcggac accttgcgca aacagtagaa ccctgctacg
     1861 accgccaggc caacgaggac ggcctgacct cccagaagcc actgcgggga cggtgttatg
     1921 gcgtagaggg gcaggaaagg caggagcagg aagtatgcgt gtgtgccgag aacggatcgg
     1981 ttctcgatgt cgttgaacag gaagccgccg ttgaacgggt gcgcaagggg ccggtcataa
     2041 aaagagaagt cccacgcatt cacctcgaac ccgtagtacc gattcctgat gaggagatat
     2101 acgagcacgg ttgctgccgc gccgccggct gcgatcagcc gacgcgagat gtgaagatct
     2161 tcccgaagga atgcgccagc actggcgctg cgcgcgacag ggaataccgc catccaaaga
     2221 atcacgagtc ccagagctgc gccgagtggt actgccaggc ggttgatctc cacgccaacg
     2281 attcgccatg gaccggtcgc cgcgaacagg agcagaaatg cgacgatcgc ccaactcaga
     2341 atggcgagcc ctgacaaccg agacggtggc ccggcaccag agttccccag catttcttcc
     2401 gccgccctgc catctcgcag cgcacccctg atcactcgct gcagtgcacg ggccatctga
     2461 ccggccagga aaaaaggaac atccgaactc tgattcggcg tgcagtcgtt cctacggctg
     2521 gatcgagagg tgggttccgc cgagcacctc gagcgccgcg cccgtgatgg cggccgcgtc
     2581 gtc
//
LOCUS       DATIFQ010000322         2728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2212433, whole genome shotgun sequence.
ACCESSION   DATIFQ010000322 DATIFQ010000000
VERSION     DATIFQ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212433"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VLE22_14995"
     CDS             complement(<1..827)
                     /locus_tag="VLE22_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15763.1"
                     /translation="MNSELAGPGGASRDPLASFRDPAGTVFTTEGRIFRAVRPDGLDD
                     LTAFLSSTAGQGAAGAGQVVRTDEVPSSDAERLLDAQGLAAGAWKLFEHERIPFPSYP
                     YEWAPEMLHAAGVLTLDLALGLLEEGLGVKDGTPYNVLFRGPDPVFVDVLSFERRDPL
                     DPTWLPYAQFARTFLLPLLAVKHFGLGLDQLLLARRDGLEPEDVYRWAGRFQRLKPPF
                     LSLVSMPTWLASRHDQDDASLYRKRSAGSEEKAKFIVGTLLRRLRRTLGRLEPGQGQ"
     gene            1176..1547
                     /locus_tag="VLE22_15000"
     CDS             1176..1547
                     /locus_tag="VLE22_15000"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB15764.1"
                     /translation="MVVDTSVLLAVFFNEKHGPWAADQLHENRHRLRMSTVNYAEVLI
                     LVKDRQPQLYNEIREAILEAGIRLVPPTATQAEAAADARLRFPLNLGDCFAYALAKDE
                     DCVLLTLDRDFAKTDVAVVLP"
     gene            complement(1567..1791)
                     /locus_tag="VLE22_15005"
     CDS             complement(1567..1791)
                     /locus_tag="VLE22_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15765.1"
                     /translation="MRTVRVSFITALLSLAVAAYWIFRSNPVPAVIWLVIAVVWLGLA
                     IKHAASEPEVEDQPFRRLARRLSRLLLYWG"
     gene            complement(1838..>2728)
                     /locus_tag="VLE22_15010"
     CDS             complement(1838..>2728)
                     /locus_tag="VLE22_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15766.1"
                     /translation="RLTVTPGIRLENYDYVRNIHRQPVGGTPTDVNIRGGDSVFKPIP
                     GIGVAYQVWSPVTLFAGAHRGFAPPRVKDAITGAGVSLQLDAELSWNYEAGARLKLPR
                     GLRAEATYFTLDFENQIIPAAQSGGATTTLINGGETLHRGAEFSLAADWSRLIDSRLG
                     LWTEVRHTWLTDARFTSGIYNGNRLPYAPEHSFGVRLGYRHRRGFSVHVDGTRVGDQF
                     GDNLETAKGSADGTVGLLPAYWVWNLCAGHEFQRERFVVNPFFTVKNLADAVYISSRA
                     PLGIQPGMFRQINGGVKLRF"
BASE COUNT          540 a          908 c          822 g          458 t
ORIGIN      
        1 tgctggcctt gacccggctc gaggcggccg agcgtccgcc gcagacggcg caacagcgta
       61 ccgacgatga atttcgcttt ctcctcgctt ccggcggacc gtttgcgata gaggctcgca
      121 tcgtcctgat cgtgcctgga cgccagccag gtcggcatcg agaccagcga caggaacggc
      181 ggcttcaacc tctggaaacg acccgcccac cggtacacgt cctcgggttc gagaccgtcc
      241 cggcgcgcca gcaacaactg gtcgaggccg agcccgaagt gcttcaccgc aagcagtgga
      301 agcaggaacg tccgcgcgaa ttgcgcgtaa ggaagccagg tcgggtcgag cgggtcgcgg
      361 cgctcaaagg aaagaacgtc cacgaacacc gggtcaggcc cccgaaacag cacgttgtaa
      421 ggagttccgt ctttgacgcc gagaccttcc tccaacaggc cgagcgcgag gtcaagcgtt
      481 aaaacgcccg ccgcgtgcag catctccggc gcccactcgt agggatagct cgggaacggg
      541 atgcgttcgt gctcgaatag cttccaggcg ccggcggcca gcccctgcgc atcgagcaac
      601 cgttccgcat cactcgaggg aacctcatcg gtacggacca cctggcccgc gccggccgcc
      661 ccctgtcccg cggtcgagga gagaaatgcc gtcagatcgt cgaggccgtc aggacggaca
      721 gcgcggaaga tccggccctc cgtcgtaaac actgtgccgg ccggatcgcg aaaggatgcc
      781 aaaggatccc gggacgcccc gcccggtccc gcaagttcgg agttcaccaa gcgacgaatt
      841 gtatggcacg ccaccgggcg gcgtcaatta caccgcgcct acccccaata caacagcagc
      901 cgtgagagcc ggccattgct ttatacaggc cctacctgta taatcagatg tatgcggcag
      961 ctaaacataa atgtcacgcc agagtttgaa cgtgaccttc tgcaatacat gaagctgcgt
     1021 agcgtcgcgc gaaaatcgga tgcgatccgt caggccctgc gtgaggcggt cgatcgccag
     1081 gctgctggtg ctgacttcga cttccggtcc tggcttggaa tgggcctgcg agctccgctc
     1141 aggcgtaaca ggcgcttccg gtccgaggac gatctgtggt cgtagatact tcagttctgc
     1201 ttgctgtctt cttcaacgag aagcacggtc cttgggcagc cgatcaactg cacgaaaacc
     1261 gccaccggct gcgaatgagc accgtgaact acgcggaagt gctgatcctg gtcaaagacc
     1321 gccagccgca gttgtacaac gaaatccgcg aagccatcct tgaagctggc atccggctcg
     1381 tcccacccac ggcaactcaa gccgaggccg cggcggacgc gcgcctgcgg tttccgttga
     1441 atctcggcga ctgctttgcc tatgcgctcg cgaaggacga ggattgtgtc ttgcttacgc
     1501 tggaccgtga cttcgcaaaa accgacgtgg ccgttgtact gccctgagga cggcccacga
     1561 aggccattag ccccaataca acagcagccg tgagagcctc cgcgccagac gtcgaaaggg
     1621 ctggtcctcg acctctggtt cggaggccgc gtgcttgatc gcgagtccga gccacacaac
     1681 agcgatgact agccagatga cggcgggtac gggattgctg cggaagatcc agtaggccgc
     1741 cactgccagt gatagaaggg ccgtgatgaa gctgacccgg acggttcgca tcgcttcgcc
     1801 tccacccaga tcatacaccc gggtccttgc cgtcgggcta aaaccgcagc ttcacaccgc
     1861 cgttgatctg ccggaacata ccgggctgga tgccaagggg cgccctcgac gagatataga
     1921 ccgcatccgc caggttcttc accgtgaaga acggattcac cacgaaccgc tcacgctgga
     1981 actcatggcc cgcgcaaagg ttccataccc agtacgccgg aagcagccca acggtgccgt
     2041 cggcagagcc ttttgccgtc tccagattgt cgccgaactg atcccccacc cgcgtgccgt
     2101 cgacgtgcac agagaagccc ctccggtgac ggtagccgag ccggaccccg aagctgtgct
     2161 ccggagcata gggtagccgg tttccattgt agataccgga ggtaaagcga gcatccgtca
     2221 gccaagtgtg cctcacttcc gtccacaggc cgaggcgcga atcgatcaac ctcgaccagt
     2281 ccgccgcgag gctgaactcg gcgccacggt gcagcgtctc gccgccgttg atgagggtcg
     2341 tcgttgcccc gcccgattgt gccgccggga tgatctggtt ctcgaaatcc aacgtgaagt
     2401 acgttgcctc ggcccgcagt ccgcgcggga gcttcagccg ggcgccggct tcgtagttcc
     2461 agctcagctc ggcgtcgagc tgaagcgaca cgcccgcgcc ggtaatggcg tccttcaccc
     2521 tcggcggcgc gaacccccgg tgcgccccgg cgaacagcgt caccggcgac cagacctggt
     2581 acgccacgcc gatccccgga atcggcttga aaaccgagtc accgccgcgg atgttcacgt
     2641 ccgtcggcgt cccgccgacc ggctgccggt gaatgttccg tacgtagtcg tagttctcga
     2701 ggcgaatacc cggcgtcacc gtcaaccg
//
LOCUS       DATIFQ010000323         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1830968, whole genome shotgun sequence.
ACCESSION   DATIFQ010000323 DATIFQ010000000
VERSION     DATIFQ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830968"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2534)
                     /locus_tag="VLE22_15015"
     CDS             complement(<1..>2534)
                     /locus_tag="VLE22_15015"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HSB15767.1"
                     /translation="AYVNPSGRVMGFEHVIPEAAAGARPNRTEARQRAEECLRRQTQA
                     PHRLIEEELRERPNRYDYVFTWEEEGFRAKDATLRRTVELHGDRVGRYSEYLYVPEQW
                     KRDFAALRSKNELYAQIAQAFYLPLVLGAIVILIRAFRRGLIRWRPLLLISLTVAGLM
                     IINQWNSLPFFLDGAPTSVPYSQSVLYGLLVALGAGVGVFFYVILAAAAGEPLYRESQ
                     LGHLSLLAVPGRRAIRTREFFVATVVGYGFAAAHIAFVVAFYLIGRRFGVWSPQDINY
                     SDFLSTKLPWIYPLTISLLASTSEEFWFRLLAIPLLKRWLRSTWLAVVIPAFVWGFLH
                     ATYPQQPGYIRGLEVGVIGVAAGFLMLRFGILATLIWHYTVDAILIGMFLIRAESWYF
                     QLSGWLVGGAVLLPLIVSLVFYLRHGGFLVDPALQNSSLATEEPRTTAAPAVEHRSTG
                     EPLAPRWQAKWLYGIAGVALVIGAAARSRDFGDFIEVQLSRIEAQVRSDAALRAHGRD
                     PGQWRRVTEFFDNLWVADFEYIRRLEGKARANEVVRERTTAGVWTTRYFRPLEKEEWR
                     VSIDSAGRLVTMDHVLDEKAPGANLTSSEARTIAERYLAGEGGVPVNRYRLADSQSEK
                     KDKRTDHSFVWEDPNFRLGEAKARVSVDVLGDEPSGVRRFLKLPEEWLREFQRPRLAA
                     IALPGVLGAAALLLLIAFVRRLSSRGSEQDSAHRYHWRVYLAAGAAAAGFAFLSAINR
                     IPGLLAGYDTARPLQSHLAEAILSRTMLVVLVGFGAFLAALAADVFLQAAAGDRRLPH
                     PSVMRALAVLSVAWCYIRGLSVMEEHLPGPHLTLPLWSLPAPETFAP"
BASE COUNT          530 a          853 c          762 g          389 t
ORIGIN      
        1 ggcgcgaacg tctcgggtgc gggcaggctc catagcggca gcgtcagatg tggtccggga
       61 agatgctctt ccattactga cagcccgcgg atataacacc aggcgaccga gagcacagcc
      121 aacgccctca tcaccgaggg gtgcggcaac cggcggtctc ccgcggctgc ttgaagaaac
      181 acgtccgcgg ccagagccgc gagaaacgct ccaaaaccca ccagcaccac cagcatcgtc
      241 cggctgagga tcgcctccgc gagatggctc tgaagcggac gcgccgtgtc gtaccccgcc
      301 agcaggccgg gaatacgatt gatggcggaa agaaacgcaa atccggcagc ggccgccccg
      361 gcagcgaggt aaacgcgcca gtgatagcga tgcgcgctgt cctgttcact cccccgagag
      421 ctcaggcgtc tgacgaaggc aatcagaagc agcaaggctg cggcgcccag cacgcccgga
      481 agcgcgatag ccgcgaggcg cgggcgctga aactcccgaa gccattcctc cggcagcttg
      541 aggaaccgcc ggacgccgga cggctcatcg cccagaacgt cgacagaaac gcgcgccttc
      601 gcttcgccga gccggaaatt gggatcttcc caaacaaaag aatggtccgt tcgcttgtcc
      661 ttcttctcgg actgtgagtc tgccagacgg taccgattca ccggcacgcc cccttcgccg
      721 gcgaggtatc gctccgcgat cgtgcgcgct tcgcttgagg tcaggttcgc gccgggcgcc
      781 ttctcgtcga gcacatggtc catcgtgacc aggcgcccgg cggaatcgat cgatacccgc
      841 cactcctcct tctcaagcgg ccggaagtag cgcgttgtcc acacacctgc cgtcgttcgc
      901 tcccggacca cctcgttcgc cctggctttc ccctccagcc gccggatgta ctcgaagtcg
      961 gccacccaaa ggttgtcaaa gaattcggtg actcggcgcc actgccccgg atccctcccg
     1021 tgggcccgca gagcggcgtc ggagcgcacc tgcgcctcga tccggctaag ctgcacctcg
     1081 atgaaatctc cgaagtccct tgaccgcgct gccgccccga ttaccagcgc aacgcccgcg
     1141 atcccgtaga gccacttcgc ctgccagcgc ggcgccaacg gttcgccggt ggaccggtgt
     1201 tcgacagcag gcgcagcagt tgtcctgggc tcctccgtcg cgagactgga attctggagg
     1261 gctggatcga caaggaaacc cccatgccgc agatagaaca caagcgagac gatcagcggc
     1321 agcagcaccg ccccgccgac cagccagccg cttaattgga agtaccaact ttccgcgcgg
     1381 atcaggaaca tgccgatcag gatcgcgtcc accgtgtagt gccagatgag cgtcgcgagg
     1441 atgccgaatc gcagcatcaa aaaaccggcc gccacgccga tgacgccgac ctccaggccc
     1501 cggatgtaac ccggctgctg cggatatgtc gcgtgcagaa agccccagac aaacgccggg
     1561 atcacgacag ccagccaagt cgagcgaagc catcgcttca gaagcgggat cgccagcaac
     1621 cggaaccaga actcctccga agttgaggcg agcagcgaga tcgtcaacgg ataaatccac
     1681 ggaagtttcg tagagaggaa gtccgagtaa ttaatgtcct gcgggctcca gactccgaaa
     1741 cggcgcccga tcagatagaa tgcaaccacg aaagcgatgt gggccgcagc gaacccgtag
     1801 ccgactaccg tggcgacgaa gaattcgcgc gtgcgaatcg cccgccgtcc gggaaccgcc
     1861 aggagagaca gatgtcccag ttgtgattcc cgatacagcg gctcgcccgc ggccgccgcg
     1921 aggataacgt agaagaagac gccgacgccg gcgcccagcg caaccagcag gccatagaga
     1981 acgctctggg aataaggcac gctggtcggc gccccgtcga ggaagaacgg gagcgagttc
     2041 cattggttga tgatcatcag cccggccact gttaacgaga tcagcaacag cgggcgccag
     2101 cgaatcaatc cccggcgaaa tgcccgaatc aggatgacga tcgcccccag taccagtggc
     2161 aggtagaaag cttgcgcgat ctgcgcatag agttcgtttt tcgagcgaag cgcggcgaag
     2221 tcccgcttcc actgttcggg cacgtagagg tattcagagt acctgccgac ccggtcgccg
     2281 tgaagctcca ccgtgcgccg caaggtcgca tctttcgctc gaaagccctc ctcctcccag
     2341 gtgaagacgt agtcatatcg gttgggccgc tcgcggagtt cttcttcgat cagacgatgc
     2401 ggcgcctgcg tctgccgccg gagacactct tctgcccgct gccgcgcctc ggttcggttg
     2461 ggccgggccc cggctgccgc ctcggggatg acatgctcga accccatgac gcgcccggac
     2521 ggattaacat acgc
//
LOCUS       DATIFQ010000324        26864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_490470, whole genome shotgun sequence.
ACCESSION   DATIFQ010000324 DATIFQ010000000
VERSION     DATIFQ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 26864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26864
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490470"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /gene="leuB"
                     /locus_tag="VLE22_15020"
     CDS             complement(<1..710)
                     /gene="leuB"
                     /locus_tag="VLE22_15020"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HSB15768.1"
                     /translation="MELNVLVLPGDGIGAEVTAEAVRVLNRVAAKYKHTLNLTEGLLG
                     GVAIHKTGQPLPAETERLALVADATLMGAVGLPEFDNAPPDKRPEKGLLGIRKALGVF
                     ANLRPVRAYPSLLDSSPLKNHLVQGTDMIIVRELTGGIYYGTPRGISGDGLETRAVNT
                     MTYTRAEIERVTRMAFHLARGRRRKVTSVDKSNVLENSQLWRKVVVETAAEFPDIELD
                     HILVDNCAMQLILNPRRF"
     gene            complement(786..1955)
                     /locus_tag="VLE22_15025"
     CDS             complement(786..1955)
                     /locus_tag="VLE22_15025"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSB15769.1"
                     /translation="MTNRVHIFDTTLRDGEQSPGCSMTVGEKLRMARKLVELGVDTLE
                     AGFPIASEGDFEAVDTISREFPSVQVAALARACQLDVDRAIRALDKAKNPRLHTFIAT
                     SDIHLKYKLKKTRQQVIEEAVAAVEQARRHVDDVEFSAEDATRTDVDYLIDVCRAVVA
                     AGAQTVNLPDTVGYSIPSEHAALIGQMVQALGDGVIVSVHCHDDLGLAVANSLAAVQA
                     GARQVECTVNGIGERAGNCSLEEIVMAIKVRQDILPFETGIVTSHLYPASQLLTELIS
                     FGPQPNKAIVGRNAFAHEAGIHQDGVLKERTTYEIMHPESVGVPESRLVLGKHSGRHA
                     LRDRCEHLGYNLTKEQLESVYQRFISAADRKKGVLDEEIGSILREVVDHRARTVN"
     gene            2134..3006
                     /locus_tag="VLE22_15030"
     CDS             2134..3006
                     /locus_tag="VLE22_15030"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSB15770.1"
                     /translation="MEFYTLQVFQTVATEKSFSRAAEKLLRTQPAVSLAIQRLENEIG
                     ERLIDRTGKDLLLTDAGRIVLEYSRRFENIRHEMENTLAELRDNAAGRLIIGANESTT
                     LYLLQHIQKYRRLYPKVKVQVRRSLSSKIPSQLIDGDLELGVISYDPADVRLVSRIIY
                     TDHLSFVVSPHHRFAGRDSVSIAELGMETFIAHNVLSPYREVVIREFQRHKIPLNMDV
                     EMPTVETIRRLVARDEGVAFLPRMCVEQEFEQGILCEVAVPELSAERKIRLVHPARRA
                     LSHAAKAFLELVKE"
     gene            3076..4020
                     /locus_tag="VLE22_15035"
     CDS             3076..4020
                     /locus_tag="VLE22_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB15771.1"
                     /translation="MGNLAEQHSLLVRGLRKAYKDVTAVAGLDLDVCPGECLGLLGPN
                     GAGKTTTLEICEGLLEPDAGRVELFGLSWNSHARELRQRLGIQLQETQLSEKLTVEET
                     VRLFRSFYRNGPAVAEVIDLVELQEKHAARVGSLSGGQKQRLAMACALVGEPDLLFLD
                     EPTTGLDPQARRQLWDLIANFKTGGRAIVLTTHYMEEAERLCDRVAIIDHGLIIAQGP
                     PRELIASIGAVHMVEFSTGGASGTPDLECLSKLDGVHHVRVENGTFQLQVSELHRTVP
                     ALLDALDHSGVPLTELRTHSATLEDVFVSLTGRHLRDE"
     gene            4013..5077
                     /locus_tag="VLE22_15040"
     CDS             4013..5077
                     /locus_tag="VLE22_15040"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB15772.1"
                     /translation="MSDFAAIAPRTQAFAGRSLVELTLVRFREFWREPEAVFWVFIFP
                     ILLAAGLGLAFRNRPADVLNIGVSTPELARSLASEKSVAARTFTLAEGEQALRAGRIA
                     LLALPGANGGVVYRYDDTNPEGRLARFLVDQALQRAAGRRDPVPAGDELVREPGSRYI
                     DFLIPGLLGMNLMGSGIWGIGFAIVDARRKKLLKRLIASPMSRAEYLLSFLFSRLVLL
                     LFEVAALVGFGIWVFGVPLRAPLWELAFLCLLSSLSFSALGLLIASRVRTIEGASGLM
                     NFVMLPMWVLSGVFFSAQRFPDVLQPFIKAMPLTAVVDALRGSMLQGARLPHLASELA
                     VIAVWLVLSFIFALKLFRWR"
     gene            5086..5457
                     /locus_tag="VLE22_15045"
     CDS             5086..5457
                     /locus_tag="VLE22_15045"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB15773.1"
                     /translation="MDTNCLTDAFRGESATVRIIEQAVEVWIPFVTLAEIKAGSLAGS
                     RTAENERLLQEFLLLPGVALLYADRETTDVYARLFIQLRRAGTPIPTNNLWIASLAVQ
                     HQLLLLSRDEHFNILPQIARA"
     gene            complement(5474..8098)
                     /locus_tag="VLE22_15050"
     CDS             complement(5474..8098)
                     /locus_tag="VLE22_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSB15774.1"
                     /translation="MTIGHLLAEAAQRRPEDAAILAPGRAPWSYGQLHDIVRNAAAGL
                     REAGVTRPDRVAVVLPNGPEMAAAFLSVASAAVCAPLNPAYGKSELLFYLSDLKARAL
                     IIEEGSESPAAEAAAELNVPVIHLRAQAGGGDDAGANEKCSEDDVALVLHTSGTTSRP
                     KIVPLTHRNLCRSADNIRRSLTLTRGDRCLNVMPLFHIHGLEAALLSSMAAGASVVAT
                     PGFHPVRFYEWLDEFTPSWYTAVPTMHQAILARAGNHRDVIERRRPRFIRSSSSALAP
                     ALMAELEQVFRAPVIEAYGMTEAAHQMTTNPLPPGHRKAGSVGLPAGVDVAIMDEAGN
                     LLPAGETGEIVIRGENVTDGYEANPEANEKAFTNGWFRTGDQGRFDAHGYLYLTGRLK
                     EIINRGGEKISPREVDEALLEHPGIAQAVTFAIPHSQLGEETGVAVVLREGAAMSERE
                     VREFAASKLAEFKVPRVVRIVREVPKGPTGKLQRIGLAQTLGIGPIDPAREGGEHPFA
                     APRSGIEEQVAAVWRKVLGTERAGRDDSFFALGGDSVLAAELLSRVTVELNVALPFLV
                     FLEEPTLAGMARRIEEAQRRGGTEERGSGSLIPIQTGGALPPLFCVGGHAGGLGAFCG
                     FARHLDREQPVYGFAPPRLEDTKHDFRIEEYAEACLEQIRTRQPEGPYYLAGLCFGGL
                     ITYEIARRLKQDGQTVALLVMMDCLRGSGGRQVPLREVVARRVRRARRRREIIFGNLA
                     RRRAGERLTYLMERLQAFFTVMKDRAGGTLYSLYRTVGLWPPISLRQARYASPFARRL
                     YSPAPYDGPVTLFRVKDLRPDIPMLGWEGLLRGEVRVFDVPFHPHGIGAEPLGPVAGS
                     LLRQAMEEAQRKVRSDAS"
     gene            complement(8095..8805)
                     /locus_tag="VLE22_15055"
     CDS             complement(8095..8805)
                     /locus_tag="VLE22_15055"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB15775.1"
                     /translation="MQSTGQQPQATAVKEEPKQPPKPVPPPEFQSEKIRTLDEAGLIQ
                     ILKDPSATTFQKAKACQRLASTGTKEAIPALAALLTDPQLGHYARYGLEPIPDPSADD
                     ALRGALPKVKGRLLAGAIDSIGKRKDAKSAGTLARLLNNADPVVAQAAAAALGNISGL
                     EAAKALQSGLGRTKGAMHRAVAGAALVCAEGLLAQGQRAQALDLYSMLARPDVPKHVR
                     LAAMHGIIAAETSLSRPR"
     gene            complement(8864..10117)
                     /locus_tag="VLE22_15060"
     CDS             complement(8864..10117)
                     /locus_tag="VLE22_15060"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15776.1"
                     /translation="MTAAAPAIVPGSVLGQRAGAVPPSDRIVMGGIGIGGRGQSDLRA
                     LLSYRQVQFVAICDVRNQRREEIKSMVDQRYSNHDCVMYDDQFELLARGDIDAVLIAT
                     GDRWHTPLSILAAQHGKDVYCEKPCSMSMEESWALADAFRRYGRIYQAGCQRRNGGNL
                     ELAVELVHTGKLGKLQTVYADVGPSVNWPPLPGHDWLPAEPEPPKQVLDWDRWLGPAP
                     WRPYNPEYVRGGWRNWYDFHGGGILEWGSHTVDLCQWAAKADDTQPVEYEPEGGNTGK
                     YFVHCRYANGVKLEMRDDGWDGSLGTGSCTVRFEGDNGWVETGDTGKIEVSENLRSEL
                     RPTTEARLALAYHTQDWLNCIKSRTQPRASAAVAANSHISCHAAFIAFQLGRKLTWDP
                     AMKEFVGDDEANRMRSRALREPWRL"
     gene            10258..11652
                     /locus_tag="VLE22_15065"
     CDS             10258..11652
                     /locus_tag="VLE22_15065"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSB15777.1"
                     /translation="MKRKLVLSYIGLVGVPLATLLVILPLGRSLSPPSPRAESWPFPV
                     LPTNLGSQGAALGISNLALLVCQIALIILLSRAVGSLAEKIRQPRVIGEVLAGILLGP
                     SLLGWLAPGVSAFLFPPSGFAVLNGLSQLGLMLFMFMVGLELNVKELRHQGPIAILIS
                     HASMAAPMALGAGLALYLYPRLSDAAVSFTGFALFMGAAMSVTAFPVLARILGERNLA
                     QTRMGTMAIACAAIDDVTGWCVLAFIAAHVRAADSSVRIWTTVAGLALFLAVMFWGVR
                     RLLRRIERAYLEHGELSDNRKAIVLLLLLLSALSTEALGLHPLFGAFVAGAVMPRNRD
                     FVAYLMEKLESLTVLLLLPLFFAYTGLRTSIGRVAGAEMWGLCLLIVATAVIGKLAGT
                     GLAARMGGMPWRESLVLGVLMNTRGLMELVVLNIGLDLGVISPALFSMMVVMAISTTM
                     MAPPLLHWLYPRLT"
     gene            complement(11679..12203)
                     /locus_tag="VLE22_15070"
     CDS             complement(11679..12203)
                     /locus_tag="VLE22_15070"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB15778.1"
                     /translation="MRKLLLSFVLGGAALHAQPAGLWQGYDGEWGHVSHQLLALAEAT
                     PAEKFGWRPALGVRSVSEVYMHIALANFYLLSVTGPELPADIKKGDLEKTVTAKAEVI
                     GWLKRSLDAVKTARAALKPGDLERKVTILKRDATVDGMYLRIIIHANEHMGQLIAYAR
                     MNGIVPPWAKSGGE"
     gene            12299..12856
                     /locus_tag="VLE22_15075"
     CDS             12299..12856
                     /locus_tag="VLE22_15075"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HSB15779.1"
                     /translation="MALVISMLRGVNVGGHNKIKMEALRSLYESLKLRDARTYVQSGN
                     VIFRADERNLVSLASRVENAIKRNFGFRPDVIVRTASELRDAIARNPFATRRSIDPSK
                     LLVVFLARDPGPEARDKVLKINADPEELRIGGRELYIHFPNGMGRSKLSVAAVERALG
                     VPGTGRNWNTVTRLLEIAEGLEASR"
     gene            13058..15448
                     /locus_tag="VLE22_15080"
     CDS             13058..15448
                     /locus_tag="VLE22_15080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HSB15780.1"
                     /translation="MHVKLRSILRRLLTTSSTSAAALFHLFRQLAFVILAGLLALPSY
                     AEPLHSLLQGTVLDPSRAPIAGATVSATTEGRVESVSTHTGKAGEFSLDLEPGSYTLH
                     IAADGFRDSSQPVAVSDTSPLPLEILLPLAPRQDMVTVTETVDYQVSTSSSTRTPIPL
                     RDVPQSISVITHDLIRDIGMQNMADVVRYVPGITMAQGEGHRDAPVIRGNVTTADFYV
                     NGVRDDVQYFRDLYNVERVEAVKGANALTFGRGGGGGVINRVTKEAQFYPVREITLQG
                     GTFSNKRFTTDFGRSITDKIAFRVNGLYENSDSFRQGVNVERYGASPTLTIKPGERTK
                     VRLSYEYLNDGRTVDRGIPSFQGRPSDANRSTFFGDPKRNYATADVNLGSAMVEYQAG
                     LFNIRNSTFLGDYDKFYQNLVPGAVDATQTLVSLSGYNNATARRNLFNQTDATGVVAT
                     GQFRHTLLIGSEFGRQHTQNFRNTAYFYDTATSTIVPFGNPRFTGPVTFRQAASDADN
                     IATNLIAAVYVQDQVELTRHLQVVGGIRYDRFTIDFHNNRNGENLGRQDNMLSPRAGL
                     VLKPIAPLSLYTSYSVAYLPSSGDQFSSLTVTTRTLKPEKFNNYEVGAKWDVSRTLSF
                     TTAVYRLDRTNTMARDPNNPAITVQTGSQRTNGYELGVNGSVTKRWALVGGYAYQDAF
                     VTRATRAAARDAQVALVPHRTLSLWNNYRVSPRLHMGLGVIHQGEMYAGIDNTVRLPQ
                     FNRADLAAYYSLTEVVHMQVNVENLFDRTYYATANSNNNIMPGYARAVRVGLVARF"
     gene            complement(15452..15874)
                     /gene="ndk"
                     /locus_tag="VLE22_15085"
     CDS             complement(15452..15874)
                     /gene="ndk"
                     /locus_tag="VLE22_15085"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSB15781.1"
                     /translation="MSQIERTLSIIKPDAVAAGQSGEILAMIEKAGFRVLGMRMVRLR
                     KQQAEGFYAVHRERPFFPNLVSFMTEGPVIVLALEREDAIVKLREVMGATNPANAADG
                     TVRKRFAANIERNCIHGSDAPATAETELRFFFSTSELL"
     gene            complement(15871..16737)
                     /gene="sucD"
                     /locus_tag="VLE22_15090"
     CDS             complement(15871..16737)
                     /gene="sucD"
                     /locus_tag="VLE22_15090"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HSB15782.1"
                     /translation="MSVLINENTRVLIQGFTGREATFHAQQCIAYGTHVVGGVVPRKG
                     GSTHLDLPVFNTVREAVLATGADASAIFVPPAFAADAIMEAADAGLSVVVCITEGIPT
                     LDMVKAWDFLQGRSTRLIGPNCPGIISPGKCKIGIMPGHIHRLGHVGVVSRSGTLTYE
                     AVHQLTLLGIGQSTCIGIGGDPIIGTNFIDAIRLFNEDPGTHAIVMIGEIGGNAEEMA
                     AAYIKEHVKKPVVGFVAGQTAPPGRRMGHAGAIISGGAGKASDKIAAMQAAGITVCAS
                     PAEIGEKVKSRL"
     gene            complement(16734..17891)
                     /gene="sucC"
                     /locus_tag="VLE22_15095"
     CDS             complement(16734..17891)
                     /gene="sucC"
                     /locus_tag="VLE22_15095"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HSB15783.1"
                     /translation="MKIHEYQAKSILAQHGVPVPRGEVVYSPEEAAIAAKKIGGSVVV
                     KAQIHAGGRGKGGGVKIAIGLDEAAEIAKNMLGMKLVTHQTGPEGRIVQRVLIEETLP
                     IERELYLGIVLDRVQGKPVFMASAAGGVDIEEVAAKSPEMILKETIEPGLGLTPFQAR
                     KLAFGMGLPAAVVGGAVAAMTALARAYAAVDASLAEINPFILTTDGKVYALDAKINLD
                     DNALYRHKDLLELRDLNEEDPLEVEASRHGLNYIKLDGTVGCMVNGAGLAMATMDIIK
                     YAGGSPANFLDVGGGANADQIKNAFRILLSDRNVKAVLINIFGGILRCDTLATGVVAA
                     ARELQVQVPVVVRMEGTNVQEGKRILTESGLNFTVADGMRDAAEKVVRLAQ"
     gene            17942..18967
                     /gene="trpD"
                     /locus_tag="VLE22_15100"
     CDS             17942..18967
                     /gene="trpD"
                     /locus_tag="VLE22_15100"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSB15784.1"
                     /translation="MTLLTYLDRMMAREDLSAHDAEEAMSTILGGEATTPQIAAFLVA
                     LRMKGETAQEILGFARAMRAKAARVDVRLSGELLVDTCGTGGDRSGTFNISTISAFVA
                     AGAGARVAKHGNRSISSRCGSADVMEGLGVKIALAPEQMARAIREVGIAFLFAPVIHV
                     AMKHAQPARLELRTRTVFNILGPLTNPAGAQAQLVGAPDEHAAELMAETLAALGLTRG
                     FVAHGSDGLDEITTTGPSLLIEIAGGTVTHRTVGPEDFGVPCARKADLLGGDLEANCE
                     IAREVLGGARGPRRDVVLVNASAALVAAGRARDFREGVAMAMESIDSGAARRRTEDLA
                     RFTQQFG"
     gene            complement(18977..19531)
                     /locus_tag="VLE22_15105"
     CDS             complement(18977..19531)
                     /locus_tag="VLE22_15105"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSB15785.1"
                     /translation="MTRRHWFPKPYADAVARLRVPSGFLLVAAFVLLSDPAPRSLTAG
                     LPIALLGLLLRGWAAGHLAKNECLATGGPYAWMRNPLYLGTLLVAAGFALASRSSLLA
                     AVLAAAFALVYLPVIELEEQHLRKLFPDYAGYAERVPMLWPRFQASASSLPFRWSLYW
                     RNQEYQAMAGFLAGVGLLVWKTWW"
     gene            complement(19528..20535)
                     /locus_tag="VLE22_15110"
     CDS             complement(19528..20535)
                     /locus_tag="VLE22_15110"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSB15786.1"
                     /translation="MYTAGSTQANSGPRILVVRLGAMGDVLHALPAVATLKHGFPNSR
                     LSWVIAPKWAPLIEGNPFVDEAILFERKTFSGLVSAWRELRHRRFDLAVDFQGLVKSA
                     IVASVARPDRIYGFHQSLLRERVAALFYSNRVLAQAPHVVDRNLELASAAGASSIVSA
                     FPVPDGTTEGELPEGGFVLANPLAGWPGKQWPLDYYSTLAGLLRRDLDLPLVLNGPPQ
                     AAGTLAQVAGPVVHLSGISGLIHATRRALAVIGIDSGPMHLAAALQKPGVAIFGPTDP
                     SRNGPYGSSFTVLRDPAATTSYKRKKEPHPAMGRISPDDVLAALRERLSVSQCQAGSA
                     R"
     gene            20534..20857
                     /locus_tag="VLE22_15115"
     CDS             20534..20857
                     /locus_tag="VLE22_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15787.1"
                     /translation="MSNQVPGELFVIGFHSNGITGLEASKQVTDRAAIRKHHVVYHRE
                     RRNQFRPRRGRQDRRDWSVHDHDQGAGARSRSGQATAVFREHRVKAAGNDANGYAAVS
                     QLPYD"
     gene            complement(20817..21350)
                     /locus_tag="VLE22_15120"
     CDS             complement(20817..21350)
                     /locus_tag="VLE22_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15788.1"
                     /translation="MFEWARRFLILVVGDFNRSSAELQALRQLGGRALPVGLGSAATK
                     RRLEQIGLDASAVGRSRKAVAEAAADRAGGCHAVLFTAGDWDEAVEAVTDVEQQYAIA
                     DGRRMMPLLDQRDERYRFLKTPAAWAAYTLARAAGADAESAKEFARQESGHTKEDPHS
                     GRGRVNRTATAKQRRSR"
     gene            complement(21366..22415)
                     /gene="waaF"
                     /locus_tag="VLE22_15125"
     CDS             complement(21366..22415)
                     /gene="waaF"
                     /locus_tag="VLE22_15125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HSB15789.1"
                     /translation="MAATPDFQRILVRSTNWVGDAVMSLPALRALRERFPKAHIAVQA
                     RPWVADLYFRESFADRVIPYAASPGARDWSSKWNAGRELSRDSFDCAILLPNSFDAAL
                     IVWLARIPSRIGYSRDARRILLTHAVPPPAAGEIPRHERYYYLELLRRAGMLEALPEA
                     EPILLDAVSAARQSGRRRFEELGLRGPVVGVSPGAAYGTAKRWLPERFAEAAARVASQ
                     QEADVAVFGSAGERGVCEQVEAGVRYAGRRAINLAGATTLAGFIDMAAVCELFLTNDS
                     GAMHIASALGVPTVAVFGATDYETTGPSGPIVRVIREPVECSPCLLRECPIDHRCMIR
                     VSAERVAAEALDLLK"
     gene            22489..22971
                     /locus_tag="VLE22_15130"
     CDS             22489..22971
                     /locus_tag="VLE22_15130"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HSB15790.1"
                     /translation="MGRFYTREELVAARAEWKRQGKSVVFTNGCYDILHPGHIRLLEK
                     ARALGDILILAINSDSSVRRLKGASRPLNPEQERAELVLALQAVDAVTVFDEDTPREL
                     IAEVLPDVLIKGADWAHFIAGREEVEAAGGKVLSTPLEPGYSTTGIVELLLTLTVQPE
                     "
     gene            22968..26429
                     /gene="mfd"
                     /locus_tag="VLE22_15135"
     CDS             22968..26429
                     /gene="mfd"
                     /locus_tag="VLE22_15135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HSB15791.1"
                     /translation="MTHPEIRNLLEGLGRHPAFQELLGRIQKEPVSRNPLSGLNPAAK
                     ALYTVLLCKAFERPVIVVVEGVRQAEALGETVETLFDLLRGGRSVPPPQVLPALDVLP
                     HQRLSPHSEISEERAVGLWRLASGVAPITITPITSALLRTEPAEFYRQLAITLRAGEE
                     VPFDAVVEHLRSVGYEHREPVEMVGEFSVRGGILDVFSPEASRPVRIEFIGDQVESMR
                     RFDVETQRSVVKTEECTLLPLSETPKSAGLFAELYEHLGREHVPNVDEAFPGWEFMAA
                     AVRPRENSLIELAEKPLVIWDEPAQLRDVANRLWERMETTEPVGTCPPDRIFFRWNEL
                     GERLGSAAQVEFTELALDPRPGPHIGTRPAMTFHGNMPVAVAETRNAVEQGSRVVFFA
                     ASAGELERLADVFREYSVPFQLGIEPSASTPQYLAEHAYLAGSAASTYLVKGLVRRGV
                     VFPEQQVTLFGSEDLFDSSELVPRPAARAHHFAFSLDLTDLKPGDYMVHSEHGVGRFL
                     GLRELSLGDQKGDFMLLEYAGEAKLYVPLPRLDLVQKYRGAGDGAPPLDRLGGTTWNR
                     TKSRVKAKMRDMADELLKLYAERKLAQGVAFSPDSNWQREFEDAFEFSETKDQLVAVQ
                     EIKRDMEVAHPMDRLLCGDVGFGKTEVAMRAVFKVLGDGRQAAVLAPTTVLCFQHYET
                     FRRRFGPFPVRVEMLSRFLSPKERKTVLADLADGKVDIVIGTHRLLSKDVQFRDLGLL
                     IVDEEQRFGVGHKERLKQIRKNVDVLTMSATPIPRTLHMSLVGLRDMSVIETPPKDRL
                     AIHTVVAHFDWDLIRTAVDQELSRGGQVYFVHNRTDSIWSRAAMIQELTPHCRVAVGH
                     GQMSEGQLEKVMLGFMKHEYDVLVCTTIIENGLDIPLANTIIVDHAERYGLSELYQLR
                     GRVGRSNRRAYAYLLVPPDTELSETARKRLAALKEFSELGAGFKIAALDLELRGAGNL
                     LGGEQHGHINAVGYDVYVKLMEETVRELKGEEVPVEVHSTLNLGLDVRIPPEYIAEEH
                     QRLRVYKKIADVGERSQADAILAELRDRFGPPPEAVSNLLEFSLLKSLAKRLGIESIE
                     RRQGALNVKFHRESRVDPARLMNLVSRTVGGQFTPAGILRVPLEGDSTPRKVLERVKD
                     CLVHLSP"
     gene            26448..>26864
                     /locus_tag="VLE22_15140"
     CDS             26448..>26864
                     /locus_tag="VLE22_15140"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HSB15792.1"
                     /translation="MVSVLCFLGTFVLAWGAFASDVRVVEVIVAKVNNEIITSSELER
                     GRRLIEAQLRRSGTGGQQLEEAVKERTKDVLRDRIDQLLLVQKGKDLEINVDSEVTKE
                     LANIQLQNKIADQDKFQQWIREQSGMPFEDFKAEMRN"
BASE COUNT         4805 a         8799 c         8126 g         5134 t
ORIGIN      
        1 tcgaaccgcc gggggttcag gatgagctgc atggcgcagt tgtcgaccag gatgtggtcc
       61 agttcgatgt ccggaaactc cgccgccgtc tcgaccacga cctttcgcca aagctgcgag
      121 ttctcgagga cgttggactt gtcgacgctc gtcaccttcc gccgccgccc gcgcgcgaga
      181 tggaacgcca ttcgcgttac ccgttcgatt tcagcgcgcg tgtaggtcat cgtgttgact
      241 gcgcgtgtct ccaagccgtc gcccgagatc cctctggggg tgccgtaata gatgccgcct
      301 gtcagctctc ggacgatgat catgtccgtt ccctgaacca ggtggttttt gagcggcgac
      361 gagtcgagca atgacgggta cgcccgcacc gggcgcaggt tggcgaacac gcccagcgcc
      421 tttcggatcc cgagcagccc cttctcgggc cgcttgtctg gcggtgcgtt gtcgaactcc
      481 ggtaggccca ccgctcccat cagagtcgcg tccgcgacca gcgcaagccg ctcggtctct
      541 gccggcagcg gttggcccgt cttgtggatg gccacgcctc ccagcagtcc ttcggtcaga
      601 ttcagtgtgt gcttgtattt ggcggccacg cggttgagaa cgcgcacagc ctccgcggtg
      661 acttcggcgc cgatgccgtc acccggcaaa acgagtacat tcagttccat ttgtttatga
      721 gctgcgagaa aggtcgcgcg gccttcgcgg aaggtgtcga ccggcgggca tctgcccggc
      781 ccttcttagt ttaccgtcct ggcgcgatga tccactactt cgcggaggat gctaccgatc
      841 tcttcgtcca gcacgccctt ctttcggtcc gccgcggaaa tgaaacgctg gtagaccgat
      901 tccaactgtt ccttcgtcag gttgtagcca aggtgctcgc aacggtcgcg aagggcgtgc
      961 cgcccgctgt gcttgcctag aaccagccgg ctctctggaa cgccgaccga ttcggggtgc
     1021 atgatctcgt acgtggtgcg ctctttcagg acgccgtctt ggtggatgcc cgcctcgtgg
     1081 gcgaaggcgt tgcgtccgac aatcgccttg ttgggctggg gaccgaatga gatcagctcg
     1141 gtaagcaact ggctggcagg atacaagtga gaagtgacga tgcccgtctc aaacggcagg
     1201 atgtcctggc gaactttgat cgccatgacg atctcttcga gcgagcagtt ccccgcccgt
     1261 tcgccgatgc cgttgaccgt gcattcgacc tgccgcgcgc cggcttgaac cgccgccagg
     1321 gagttcgcca ccgccagccc gagatcgtcg tggcagtgta cgctcacgat gaccccgtcg
     1381 ccgagcgcct gcaccatctg cccgatcagg gccgcgtgct cgctcggaat ggaatacccc
     1441 accgtgtccg gtaggttcac ggtttgggcg ccggcggcga cgaccgccct gcaaacgtcg
     1501 atgagatagt cgacgtcggt gcgggtcgca tcctccgccg aaaattccac atcgtcgacg
     1561 tgacggcgcg cctgttcgac cgccgccacc gcctcttcaa tcacctgttg ccgcgtcttc
     1621 ttcagcttgt acttcaggtg gatgtcgctc gtcgcgataa atgtatgaag gcgaggattc
     1681 ttggctttat ccagagcgcg gatggcccgg tccacgtcca gttggcaagc tctcgccaaa
     1741 gccgccacct ggacagaggg gaactcacgg ctgatggtgt caaccgcttc gaaatcgccc
     1801 tctgacgcga tcggaaagcc ggcttcgagg gtatcgacgc ccagctccac caatttccgc
     1861 gccatacgga gcttttcccc taccgtcatg ctgcatccgg gcgactgttc gccgtcccgc
     1921 agcgtggtgt cgaatatatg tacgcgattc gtcatggacc atccgcgctt tggaagccag
     1981 ccccgtacgg aatgttcctc cggatacggg gacgacaatt tccattattg ccgtggacct
     2041 atggatttga caaatttata atgttcacac ttcgcatcaa gataaatgat tcgataaact
     2101 tggcttatac tgtcccaaag gagtatgagt cgaatggagt tctacacgct gcaggtgttc
     2161 caaaccgtcg cgacggaaaa aagcttctcc cgcgccgctg agaagctcct gcggacccag
     2221 cctgccgtct ccctcgccat ccagcgcttg gaaaacgaga tcggagaacg actgatcgat
     2281 cgcactggga aagatcttct cctcaccgac gccggccgga tcgtcctcga atactcgcgc
     2341 cgtttcgaga acattcgaca cgagatggaa aacaccctcg ccgaacttcg tgacaacgcg
     2401 gccggtcgcc tcatcatcgg cgccaacgag tccaccaccc tctacctcct ccagcacatc
     2461 cagaagtatc gccggctgta cccgaaggtg aaggtccagg tacgccgaag cctttcgagc
     2521 aagattccca gccagttgat cgacggcgat cttgaactcg gcgtcatcag ctatgacccc
     2581 gccgacgtcc gccttgtctc ccggatcatc tacaccgacc atctctcctt cgtcgtctca
     2641 ccccatcacc gcttcgccgg ccgtgattcg gtctccattg ccgaactcgg catggaaact
     2701 ttcatcgctc acaacgtgct ctcgccgtac cgggaagtcg tgatccgcga gttccagcgg
     2761 cacaagatcc ctctcaacat ggatgtcgag atgcccaccg tggagaccat ccgccgcctg
     2821 gtcgctcgtg acgagggtgt cgcgttcctt ccgagaatgt gcgtggaaca ggagttcgag
     2881 cagggtatcc tctgtgaggt ggccgttccg gaactcagcg ccgagcgcaa gattcgcctg
     2941 gttcaccccg cccgccgcgc tctcagccac gccgctaagg ccttcctcga gctcgtcaag
     3001 gagtaagcgc gcgccgcacc tccctcgcgc ggtgtttgcg ctcgtgtctc accgcacgcg
     3061 atactggttt aaagcatggg caatctcgct gagcagcact cgctgctcgt tcgcgggctg
     3121 cggaaggcgt acaaagatgt caccgccgtc gccggccttg atcttgatgt gtgccccggt
     3181 gagtgtctcg gcctcctcgg ccccaacggc gccggcaaaa ccactaccct cgagatttgc
     3241 gagggcctcc tagaacccga tgccggccgc gtcgagctct tcggattgag ctggaactcc
     3301 cacgcgcgcg agcttcgcca gcgtctcggc atccagcttc aggagacaca gctctccgaa
     3361 aaactcaccg tcgaggagac cgtccgtctt ttccgcagct tttaccggaa cggtcccgcc
     3421 gtcgccgagg tcatagacct ggtcgagctt caggaaaagc acgccgcccg tgtcggttct
     3481 ctctccggcg gccaaaagca gcgcctcgcc atggcctgcg cgctcgtcgg cgagcccgac
     3541 ctcctctttc tcgacgagcc aactaccggc cttgatccgc aggcccgccg ccagctttgg
     3601 gatcttatcg cgaacttcaa aaccggcgga cgcgctatcg ttctcactac tcactatatg
     3661 gaggaggcgg agcgcctgtg cgaccgcgtc gcgatcatcg accacggcct catcatcgcc
     3721 caaggccccc cccgcgagct catcgcctcc atcggcgccg tgcatatggt cgaattctcc
     3781 accggcggcg cttccggcac cccggatctg gaatgcctgt cgaagctcga cggcgtccat
     3841 cacgtccgcg tcgagaatgg caccttccag ctccaggtct ccgagcttca ccgcaccgtg
     3901 cccgcccttc ttgatgcgct cgaccacagt ggcgtcccgc tcaccgagct tcgtacccac
     3961 tccgcgaccc tggaagacgt tttcgtctcg ctcaccggga ggcacctgcg agatgagtga
     4021 ctttgccgca atcgcgcccc gcacgcaggc cttcgccggc cgctcgctcg tcgagctgac
     4081 tctcgtccgc ttccgcgagt tctggcgcga gccggaggcc gtcttctggg tcttcatttt
     4141 ccccatcctg ctggccgccg gcctcggtct cgcctttcgt aatcgccccg ccgacgtgct
     4201 gaatatcggc gtctccacgc ccgaactcgc ccgctcgctt gcctcggaga aatccgtcgc
     4261 ggcccggact ttcacgcttg ccgaaggcga acaggccctt cgggccggac gtattgccct
     4321 tctcgccttg cccggcgcca acggcggagt cgtctaccgc tacgacgaca ccaaccccga
     4381 gggtcgcttg gcgcgatttc tcgttgatca ggccctgcaa cgcgccgccg gccgccgcga
     4441 tcctgtcccc gccggcgacg agcttgtccg agagcctggc tcccgttaca ttgatttcct
     4501 gatccccggt ctcctgggca tgaaccttat gggcagcggc atctggggca tcggcttcgc
     4561 gatcgtcgac gcgcgccgca agaagctgct aaagcgtctc atcgcttcgc cgatgtcccg
     4621 cgccgagtac ctgctctcct ttctgttttc ccggctggtg ctgcttctgt ttgaagtcgc
     4681 tgcgctggta ggtttcggca tctgggtctt cggcgtcccg ctccgtgcgc cgctctggga
     4741 gctggccttt ctctgcctgc tctcgtcgct ctccttcagc gcccttggct tgctgatcgc
     4801 ctcccgagtc cgcaccatcg agggcgcttc gggattgatg aacttcgtaa tgctgccgat
     4861 gtgggtcttg tccggcgtct tcttctcggc gcagcgcttc cccgacgtac ttcagccgtt
     4921 catcaaggcg atgcctttga ccgccgtcgt agacgccctg cgcggaagca tgctccaggg
     4981 ggcccgcctt ccgcacctcg cctccgaact cgctgtcatc gccgtttggc tcgttctcag
     5041 cttcatcttc gccctcaagc tcttccgctg gcgctgaagc agctgttgga cacgaattgc
     5101 ctcaccgacg cctttcgtgg ggagtcggcg acagtgagaa tcatcgagca ggcggtcgag
     5161 gtctggattc cgttcgtcac gctcgctgag atcaaggccg gatctctggc cggcagccgc
     5221 actgccgaga acgagcgcct gctccaggag tttctcctgc tgcccggcgt cgccctgctc
     5281 tatgcggatc gcgagacaac cgacgtctat gcccgtttgt tcattcagct tcgccgcgcc
     5341 ggaacgccca ttcccaccaa taatctctgg atcgccagtt tggccgtcca gcaccaactt
     5401 ctgttgctat cccgcgatga gcacttcaac attcttccgc agatcgcccg tgcctgactc
     5461 ctgaagagcg gtttcagctt gcgtcgctcc gcacttttcg ctgcgcttct tccattgctt
     5521 gccgcagcag cgaaccggcc accggaccca gcggctcggc gcctatcccg tgcggatgaa
     5581 agggaacgtc gaagacacgc acctctcccc ggagcagccc ctcccatccc agcatcggaa
     5641 tatcgggtcg gaggtccttc acgcggaaca acgtgaccgg gccatcgtac ggcgcggggc
     5701 tgtacaggcg cctcgcgaat gggctcgcgt agcgcgcctg acgcagggat atgggcggcc
     5761 acaaacccac tgtccggtac aggctgtaca aggttccccc ggcacggtct ttcatcaccg
     5821 tgaagaacgc ctgcaacctc tccataaggt acgtcaagcg ctctcctgcc cgcctccgag
     5881 ccaggttccc gaaaatgatt tccctccgcc gcctcgctcg ccggaccctt cgtgcgacga
     5941 cttcccgcag cggaacttgg cgcccaccgc tgccgcgaag acagtccatc atcaccagca
     6001 acgcgacggt ttgtccgtcc tgcttcagtc gcctggcgat ttcgtaggtg atcagacctc
     6061 cgaaacacaa ccccgccagg tagtacggtc cctctggctg gcgcgtgcgt atctgttcca
     6121 ggcaagcctc tgcatactcc tcgatccgaa agtcgtgctt ggtgtcctcc agacgtggcg
     6181 gcgcaaaacc gtataccggt tgctcccggt cgagatgccg cgcgaagccg caaaacgcgc
     6241 ccagaccgcc tgcgtgcccg ccgacgcaga acagtggcgg caacgcgcca ccggtctgga
     6301 tcgggatcaa cgagccgctc ccccgttcct ctgtcccgcc tctacgctgg gcttcctcga
     6361 tgcgccgcgc catccccgca agggtcggct cctccagaaa aaccagaaac ggcaaggcta
     6421 cgttcagctc gacggttacg cgggacagca actcggccgc caacaccgag tctcctccca
     6481 gcgcgaagaa actgtcatcc ctccccgccc gttcggttcc aagaaccttc cgccagacgg
     6541 ccgcaacttg ctcctcgatg ccgcttcgcg gagcagcgaa cggatgctcg cccccttctc
     6601 gcgcggggtc gatcggcccg atgccaagcg tttgagccag cccaatgcgc tgcaacttac
     6661 ccgtagggcc tttcggtacc tcccgcacga tgcggaccac cctcggcact ttgaattcgg
     6721 ccagtttcga tgctgcgaat tcgcgaacct cccgctcgct catggcggcg ccctcgcgca
     6781 acactacggc tacgccggtc tcctcgccga gttgactgtg gggaatagcg aacgtgacgg
     6841 cctgagcgat cccgggatgc tccagcagcg cttcgtccac ctccctcggg gaaatcttct
     6901 ccccgccgcg attgatgatc tctttcagcc gcccggtgag atacagatag ccgtgggcgt
     6961 cgaaccgtcc ctggtcgccg gtccgaaacc acccgtttgt gaacgctttt tcgttcgcct
     7021 cgggatttgc ctcgtaaccg tccgtcacgt tttcgccgcg aatcacgatc tctccggtct
     7081 cccctgccgg caacaggttg cccgcctcat ccataatcgc gacatcgacg ccggccggca
     7141 gtccgacaga ccccgctttc cggtgccccg ggggaagcgg gttggtcgtc atctggtgtg
     7201 ccgcctctgt catcccgtac gcctcgatca ctggcgcgcg gaaaacctgc tccagttccg
     7261 ccatcagtgc cggagccagc gctgacgagg acgaccggat gaagcgcggc ctccgccgct
     7321 cgatcacgtc ccgatggttc cctgcccgcg ccagaatggc ttggtgcatc gtcggcaccg
     7381 ccgtatacca actcggcgtg aattcatcga gccactcgta gaaccgcacc ggatgaaacc
     7441 ccggcgtcgc tacgacgctc gcccctgccg ccatcgacga gagcagggca gcctcgagcc
     7501 cgtgaatgtg gaacagcggc atcacgttca ggcagcggtc cccgcgggtc aaagtcagcg
     7561 aacgccggat gttatcggcc gagcggcaca ggttgcggtg agttagcggt acgatctttg
     7621 gccgcgacgt cgtgcccgag gtgtggagga cgagggccac gtcgtcttcc gagcacttct
     7681 catttgctcc cgcgtcgtcc cctccacctg cctgcgccct aaggtggatg accggaacgt
     7741 tcagttcggc cgccgcttcg gccgccggcg attctgaacc ctcctcgatg atcagagccc
     7801 gcgctttgag atcggacaga taaaagagga gctcactctt cccgtacgcc ggattcagcg
     7861 gcgcgcacac ggccgcggac gccacggaca agaatgcggc cgccatctcc ggaccgttcg
     7921 gaagtaccac ggccacccgg tcgggtcgcg tgacgcccgc ctcgcgcagt cctgccgctg
     7981 cgttccgaac aatgtcatgg agctgtccgt aagaccacgg ggcgcgcccc ggagccaata
     8041 ttgcagcgtc ctccggtctg cgttgtgcgg cttcggcgag tagatgcccg atcgtcacct
     8101 gggacggctc aacgacgtct ccgcggcgat gatcccgtgc atcgcggcca gccgcacgtg
     8161 tttcggaacg tccggacgcg ccagcatgct gtaaaggtcc agcgcctgag ctcgctgccc
     8221 ctgagccagg agcccttcgg cgcacacaag ggcggcgcct gccaccgccc tgtgcatcgc
     8281 gcctttcgtc cggcccaacc cgctttgaag cgctttcgcc gcctcaagcc cgctgatgtt
     8341 ccccaacgcc gccgcggccg cttgcgccac caccggatcg gcgttgttga gcaggcgggc
     8401 cagcgtcccc gccgacttcg cgtccttccg cttcccgatc gaatcgatcg cgccggccag
     8461 cagtctgccc ttcaccttcg gaagcgcacc gcgcagggcg tcgtccgccg acggatccgg
     8521 aatcggctcc aggccgtaac gcgcgtagtg cccgagctgc ggatcggtca gcagcgccgc
     8581 cagcgccgga atcgcttcct ttgtgcccgt ggatgccagc cgctggcagg ccttagcctt
     8641 ctgaaacgtc gtcgcgctgg ggtctttcaa aatctggatg agacccgcct cgtccagcgt
     8701 ccggatcttc tctgattgaa actccggcgg agggaccggc tttggcggct gcttcggctc
     8761 ttccttcacg gcggtcgcct gaggttgctg tccggtgctc tgcattttcc tcgtctcctt
     8821 cgttctcggt ggcacaggct tcagccaggc ttcagcctgt gacctacagc cgccacggct
     8881 cccgcagcgc tctcgatcgc atgcggttgg cctcgtcgtc tccaacaaac tccttcatcg
     8941 cagggtccca cgtcagcttg cggccaagct ggaacgcgat gaacgccgca tggcaactga
     9001 tgtgtgagtt agccgcgacc gcggcgctgg cccgcggctg tgtccgcgac ttaatgcagt
     9061 tcagccagtc ctgcgtgtga tacgccagcg cgagtcttgc ctcggtcgtg ggcctcagtt
     9121 ccgagcgaag attctcggag acttcgattt tgccagtgtc gccggtctcc acccacccgt
     9181 tgtcgccctc gaagcgtaca gtgcacgagc cggtgccgag gcttccgtcc cagccatcgt
     9241 cgcgcatctc cagcttcacg ccattcgcgt accggcagtg gacgaagtac ttcccggtgt
     9301 tcccgccctc gggctcgtat tcgaccggct gggtatcgtc cgccttcgcc gcccactggc
     9361 acaaatcgac ggtgtgggag ccccactcga gaatcccgcc gccgtgaaag tcgtaccagt
     9421 tgcgccatcc gccgcgcacg tactccggat tgtacggtcg ccacggcgcc gggccgagcc
     9481 accggtccca atcgagcacc tgtttcggcg gctccggttc cgccggcagc cagtcgtggc
     9541 cgggcagcgg tggccagttc accgaaggac cgacgtcggc gtagaccgtt tggagcttcc
     9601 cgagcttccc cgtgtggacc agttccacgg ccagttccag gttgccgcca ttgcgccgct
     9661 ggcagcctgc ctggtagatc ctcccgtaac ggcggaacgc gtccgccagc gcccaactct
     9721 cctccatcga catcgagcag ggcttctcgc aatagacgtc cttgccgtgc tgtgccgcca
     9781 ggatcgacag cggcgtgtgc cagcggtccc cggttgcgat caaaacagcg tcgatgtccc
     9841 cgcgtgccag caactcgaac tgatcgtcgt acatgacgca gtcgtggttg ctgtaacgct
     9901 ggtccaccat gcttttgatc tcttcgcgcc gctggtttcg cacgtcgcag atggcgacga
     9961 actgcacctg ccggtagctg agcagcgccc tcaggtcgga ttgccctcgc ccgccgatcc
    10021 cgatgccgcc catcacgatg cggtcgctcg gaggcaccgc gcccgcgcgc tggcccagca
    10081 ccgaaccggg cacaatggct ggcgccgccg cagtcattat gaatcctcgc cttgtcatca
    10141 atggtcctcc gtcacgagag cgccattata tcgaatcgga gacagagccc tattcctctc
    10201 cccggcttcg gttcgggcta ccatgtctgg atattgcccg tcattaccca gcccgtcatg
    10261 aaacggaaac tggtgctttc ctacattggc ctcgtgggag tgccgctcgc cacgctgctc
    10321 gtgatcctcc ccttggggcg aagcctttcg ccgccttccc caagggcgga aagctggccg
    10381 tttcccgtcc tccccacgaa cctcggcagt cagggcgccg cgctgggcat ctcgaatctg
    10441 gccctcctgg tttgtcaaat cgccttgatc atcctcttgt cccgcgccgt cgggtctctc
    10501 gccgagaaga tccgccagcc cagagtgatc ggcgaggtgc tggcggggat tctgctcggc
    10561 ccctccctgc tgggctggct ggctcccgga gtatcggcgt ttcttttccc gccttccggc
    10621 ttcgctgtcc tgaatgggct cagccagctc gggctcatgc tcttcatgtt tatggtcggc
    10681 cttgagctca acgtgaagga gttgcgccat caggggccca tcgcgatcct gatcagccac
    10741 gccagcatgg ctgcccccat ggcgctcggc gcagggctcg ccctctatct ttatccccgg
    10801 ctgtccgatg cggccgtgag cttcaccggg tttgccctgt tcatgggagc cgccatgagc
    10861 gtcaccgcct ttcccgtgct ggcccgcatc ctgggcgagc gcaatctggc gcaaacccgc
    10921 atgggtacga tggccattgc ctgcgccgcc atcgatgacg tgacgggatg gtgtgtgctt
    10981 gccttcatcg cggcccacgt tcgcgccgcc gattcctccg tccggatctg gacaaccgtc
    11041 gccggactcg cgctgttcct ggcggtcatg ttctggggag ttcggaggtt gctgcggagg
    11101 atcgaacgcg cctacctcga gcatggagaa ctgtcggaca accggaaagc gatcgtcctg
    11161 ctgctcttgc tactttcggc cctttccacc gaggcgctcg gactccaccc gctcttcggt
    11221 gcattcgtcg ccggcgcggt catgcccagg aaccgcgatt tcgtcgcgta tctgatggag
    11281 aagttggagt ccctcacggt cctgctgctt cttccgctgt ttttcgctta caccggcttg
    11341 cgcaccagca tcgggcgggt ggcgggggcc gaaatgtggg gactctgcct gctgatcgtg
    11401 gccaccgccg tcatcgggaa actcgccgga accggcctcg ccgcgcgcat gggaggcatg
    11461 ccatggcggg aatcgctcgt cctcggcgta ctgatgaaca ctcgcgggct gatggagctc
    11521 gtggtgttga acatcggcct cgacctcgga gtcatctcac ccgcgctctt ctcgatgatg
    11581 gtcgtcatgg ccatttcgac cacgatgatg gcgccccctc tcctgcattg gctctacccg
    11641 cgtcttactt aattcggggc tacgcaactc cgaaactcct actccccgcc actcttcgcc
    11701 cacggtggca caatgccgtt catccgggca tatgcgatca actgccccat gtgctcgttg
    11761 gcatggatga tgatccgcag atacataccg tcgacagtgg cgtccctttt gagaatcgtg
    11821 actttgcgct cgaggtcgcc cggtttcagt gcagcgcggg ccgttttgac cgcatcgagc
    11881 gagcgcttca gccagccgat tacctccgcc ttcgcggtca cggtcttctc caggtcgccc
    11941 ttcttgatgt cagcaggcag ttccgggccc gtgacgctca acaggtagaa attcgcaagc
    12001 gcaatgtgca tgtagacctc gctgaccgac cgcacaccca gcgccggccg ccacccgaac
    12061 ttttcggccg gtgtcgcctc ggccagcgcg aggagttgat gcgaaacgtg cccccactcg
    12121 ccgtcatagc cttgccagag accggccggc tgagcgtgca acgccgcgcc gcccaacacg
    12181 aaactcagca gtaacttccg catgttttcc tccccgtcgc gccccgcctg gcgcgcgcat
    12241 atctcagcct accgcgtccg gtcgatgtcc attccgccgg gatatcctgg agcttgatat
    12301 ggctctggtg atttccatgc tccgtggcgt caacgtgggc ggccacaaca agatcaagat
    12361 ggaggcgctc cggtcgctct acgaatccct caaactgcgg gacgcgcgca cgtacgtcca
    12421 aagcggcaac gtcattttcc gtgcggacga gcggaacctt gtctcactgg ctagccgcgt
    12481 tgagaacgcg atcaagcgga acttcggctt tcgtccggac gtcattgttc gcaccgcttc
    12541 cgaactgcga gacgcaattg cgagaaaccc gttcgcgacg cggcgcagca tcgatccgag
    12601 caagctcctg gtcgtcttcc tggcccggga tcccggtccg gaagcgcgag acaaggtttt
    12661 gaagatcaat gcggatccgg aagaactgcg gatcggcggc cgcgagctct acattcactt
    12721 ccctaacggg atgggacggt cgaagctgtc ggtggccgcc gtcgagagag cgctgggggt
    12781 cccgggaaca ggcagaaact ggaacaccgt cacaaggttg ctcgagatcg cggaggggct
    12841 ggaagcctcc cgctgatccg caaaacctgg gactaaccta gggtccgccg ttcgccgggc
    12901 cccaagtcgt ccaacccgcg tttgtccggc gaggctccca ctcttttcgt gcctgacccg
    12961 tcccggtcgt gtataattcc tgttgaaagt gatttgcatg tcatacacca cgaccctgaa
    13021 caatcttaag ccttgtgtct cttatgctgc aactgaaatg catgtgaagc tccggtcgat
    13081 tctccggcgt ttgctgacga cttcttccac ttccgctgcg gcgctgtttc atctcttccg
    13141 ccaacttgcc tttgtgattc tcgctggact cctggcgctc ccttcgtacg ccgaaccgct
    13201 gcattctttg ctgcaaggaa cagtgctcga cccgagccgc gcgccaatag ccggcgcgac
    13261 agtctcggcg acgacggagg gccgcgttga gagtgtctca actcataccg gcaaggcggg
    13321 tgagttctca ctggatctcg agcccggcag ctacacgctg cacatcgcgg cggacggctt
    13381 tcgggactcc agccagccgg tggcggtgag cgacaccagt cccttgccac tcgaaatcct
    13441 tttgcctctg gcgccgcgcc aggatatggt gacggtcact gagacagttg actaccaagt
    13501 ctcgacaagc agctcgaccc gaacgccgat tccgctgcgc gatgtgccgc agtcaatctc
    13561 ggtgatcacc cacgacctga ttcgcgacat cggcatgcag aatatggccg atgtcgtgcg
    13621 ttatgttccg ggcatcacaa tggcgcaggg cgaaggtcac cgcgatgcgc cggtcatccg
    13681 cggcaatgtg accacggcgg atttctatgt caacggggtg cgagacgacg tgcagtactt
    13741 tcgcgacttg tacaacgtcg aacgcgtcga agcggtcaaa ggcgctaacg cactcacatt
    13801 cgggcgcggc ggtggcggcg gcgtcatcaa ccgcgtgacg aaggaggcgc agttctaccc
    13861 cgtacgagag attaccctgc agggcgggac gtttagcaac aagcgcttca cgaccgattt
    13921 tggccgcagc attaccgaca agattgcgtt tcgggtgaac ggcctttatg agaactccga
    13981 cagtttccgg cagggcgtga acgttgagcg atacggcgcc tcgccgactt tgaccatcaa
    14041 gcccggcgag cgcacaaagg tgcggctcag ctacgagtac ctcaacgacg ggcggactgt
    14101 ggatcgcggc attccgtcat ttcaagggag gccgtccgac gccaaccgct ccactttctt
    14161 cggcgaccca aagcgcaact atgcgaccgc cgacgtgaac ctcggctcgg cgatggttga
    14221 gtaccaggcc gggctgttca acatccgcaa tagcacattt ctgggcgact acgacaagtt
    14281 ctaccagaac ctcgtgcccg gggcggtgga tgcgacgcag actcttgtaa gcctctcggg
    14341 ttataacaac gcgacggcgc ggcggaatct gttcaaccaa accgatgcga cgggcgtggt
    14401 cgcgaccggc cagttccgcc acacgttgct catcggtagc gagttcggcc ggcagcacac
    14461 ccaaaatttc cggaacaccg cctatttcta cgacaccgcg acatcgacca tagtgccgtt
    14521 cggcaatcct cgcttcactg gcccggtcac attccgccaa gctgcatcgg acgcagacaa
    14581 cattgcgacc aaccttatag ccgcagtcta cgtgcaggac caggtcgagc tgacccggca
    14641 cctacaggtg gtcgggggta ttcgctatga ccgcttcacc atcgactttc acaacaaccg
    14701 gaacggggag aacctgggcc ggcaggacaa tatgttgtcg ccacgcgccg gccttgtact
    14761 caaaccgatc gcaccgctct ccttgtacac gagctacagc gtggcgtacc tgccgagttc
    14821 gggtgaccag ttctcgtcat tgaccgtgac cacgcggacg ctcaagccgg agaagttcaa
    14881 caactatgag gtaggcgcca agtgggacgt ttcccgtaca ctgtccttca caacggccgt
    14941 ttaccgcctg gatcgcacga atacgatggc gcgcgaccct aacaacccgg ccatcacggt
    15001 gcagaccggg agccagcgca caaatgggta cgaactgggc gttaatggca gcgtgacaaa
    15061 acgttgggct ctagtgggcg gctacgcgta ccaggatgca tttgtgacaa gagctaccag
    15121 ggctgcggcg cgtgatgcgc aggtggcgct ggttccacat cgtacgttgt cgctatggaa
    15181 caactatcgt gtgtcgcctc gcctgcacat ggggctagga gtcatccacc agggcgaaat
    15241 gtacgcgggt atcgacaaca ccgtgcggct gccgcagttc aaccgcgccg acctcgcggc
    15301 ttactactca ctcacagagg tagtgcacat gcaagtcaat gttgagaacc tgttcgaccg
    15361 cacctattac gcaacggcaa acagtaataa caacatcatg ccgggctatg cgcgtgcggt
    15421 gcgtgtcggc ctcgtggccc gattctagcc gttacagcag ttcagaggta ctaaaaaaga
    15481 agcggagttc agtttccgcc gtcgcggggg cgtcggatcc gtggatgcag ttacgttcga
    15541 tgttggcggc aaagcgcttg cggaccgtcc cgtcggcggc gttggccggg ttcgtggcgc
    15601 ccatcacctc ccgaagcttc acgatcgcgt cttcgcgctc gagggccagt acgatcaccg
    15661 gaccttcggt catgaagctc actaggttcg ggaagaacgg tctctcccgg tgtacggcgt
    15721 aaaagccctc ggcctgctgc ttccgcaggc gcaccatccg cattccaagg acgcggaacc
    15781 cggccttttc gatcatggcg agaatctcgc ccgactgccc ggccgcgaca gcatcgggct
    15841 taataatgga taacgtgcgt tcgatctgac tcataagcga ctttttactt tctctccaat
    15901 ctcagcgggc gaggcgcaca cggtgatgcc ggcggcctgc atggcggcaa tcttgtcaga
    15961 ggctttgccc gcgccgcccg aaatgattgc gcccgcgtgc cccatccggc gcccgggagg
    16021 ggccgtctga ccggcgacga agcccacgac gggtttcttc acgtgctcct tgatgtaagc
    16081 cgcggccatc tcctcggcgt tccctccgat ttcgccgatc atcacaattg cgtgcgtgcc
    16141 aggatcttcg ttgaacagcc ggatggcatc gatgaagttg gtcccgataa tggggtcgcc
    16201 accaataccg atacacgtag actgtccgat gccgagcaag gtcaactggt ggaccgcctc
    16261 gtaggtcagg gtgcccgagc gggagacgac gccgacgtgg cccagtcggt ggatgtggcc
    16321 gggcatgatg ccgatcttgc acttgccggg cgagatgatc ccgggacagt tgggaccgat
    16381 taggcgtgtg gaacgaccct gaaggaagtc ccaagctttc accatgtcga gagtaggaat
    16441 gccttcggtg atgcagacta cgaccgacag gccggcatcc gccgcctcca tgatggcgtc
    16501 ggcggcgaag gcgggcggca cgaatatcgc cgacgcatcg gcgccagtcg ccaggactgc
    16561 ctcgcggacg gtattgaaca ctgggagatc gagatgggtc gagccgcctt tacgcggcac
    16621 cacgcccccg acgacgtgcg tgccgtacgc aatgcactgc tgggcgtgga acgtcgcctc
    16681 acgaccggtg aaaccctgga tcagcacacg ggtgttttcg ttaatcagga cgctcattgt
    16741 gccagcctca ccactttctc ggctgcgtcg cgcatgccgt cggcgaccgt gaaattcagt
    16801 ccggactcgg tcagaatccg cttgccttcc tggacattcg tgccctccat gcgaacgacg
    16861 acggggacct gaacttgcag ttcgcgcgcc gctgcaacga cgccggtggc cagcgtgtcg
    16921 caccgcagga ttccgccgaa gatgttgatg agcacggcct taacattgcg gtcggagaga
    16981 aggatgcgaa acgcgttctt gatctggtcg gcgttggcgc cgccgccgac atcgaggaag
    17041 ttcgccggac tgccgcccgc gtacttgatg atgtccatgg tggccatcgc aaggccggcg
    17101 ccgttgacca tgcagccgac cgtcccgtcg agcttgatgt agttcagccc gtgtcgggaa
    17161 gcttcgacct cgagcggatc ctcctcgttg aggtcgcgta attccagcaa atccttgtgc
    17221 ctatacaacg cgttatcgtc gaggttgatc ttggcgtcta gcgcgtagac cttgccatcc
    17281 gtggtgagga tgaacgggtt aatctcggct agtgaggcgt ccacagcggc gtaggccctg
    17341 gccagagccg tcatcgcagc gaccgcaccc ccgaccacag ccgccggaag gcccatacca
    17401 aaggcgagtt tacgcgcctg gaacggggtc agacccaggc cgggctcgat ggtttccttc
    17461 agaatcatct cgggactctt ggctgcgact tcctcgatgt cgacgccgcc cgccgccgaa
    17521 gccatgaaca caggtttgcc ctgaacgcgg tcgagaacga tgccaaggta gagttcacgc
    17581 tcaatcggaa gggtctcctc gatcaggacc cgctgcacga tgcgtccctc agggccggtt
    17641 tggtgggtga cgagcttcat gccgagcatg ttcttggcga tttccgccgc ctcgtccaac
    17701 cctatggcga tcttgactcc accgcccttg ccccgtccac cggcgtggat ttgcgctttg
    17761 acgaccacac tgccgccaat tttcttagca gcgatggcag cctcttccgg agagtagacg
    17821 acctcccccc gcggcacagg gacgccgtgc tgggccaaga tactcttggc ctgatactca
    17881 tgaattttca ttggaaagca cctgtgctag gattttgaag aaccaaaacc ttcactataa
    17941 catgacgtta ctgacatacc tggaccgcat gatggcccgg gaagacctgt ctgctcacga
    18001 cgccgaggag gcgatgtcga ccatcctcgg cggtgaagcc acgacgccgc agatcgcagc
    18061 ctttctggtc gcgctccgga tgaagggtga aaccgcgcaa gagatactgg gcttcgcgcg
    18121 cgccatgagg gccaaggcgg cgcgtgtgga tgtgcgcctc agcggggagc tactggtaga
    18181 tacgtgcggc acgggcgggg accgctccgg aaccttcaac atttccacca tttcagcgtt
    18241 cgtggccgcg ggtgcggggg cgcgcgtcgc caaacacggc aaccgttcga tctctagccg
    18301 ctgcggcagc gcagacgtga tggagggact tggagtgaag atcgcgctgg cgccggaaca
    18361 gatggctcgt gcaatccgcg aggtgggcat tgcatttctg ttcgctccgg tgatccacgt
    18421 cgctatgaaa cacgcacaac cggcgcggct ggaattaaga acgcgcacgg tgttcaacat
    18481 cctgggtccg ctgaccaacc cggctggagc gcaggcgcaa ctggtcggcg cgccggatga
    18541 gcacgcggcg gagttgatgg ccgagactct ggccgccctt ggccttaccc gggggttcgt
    18601 ggcccacggc tccgatggtc tcgacgagat caccacgacc gggccgtccc ttctgatcga
    18661 gatcgcaggc ggaaccgtga cccaccggac cgtcggcccc gaggatttcg gggtgccatg
    18721 cgcgcggaaa gccgaccttc tcggcgggga cctggaggca aactgcgaaa ttgccagaga
    18781 ggtgctcggc ggagcgcgtg gtccccggcg ggatgtcgtg ctggtgaacg catcggcggc
    18841 gttggttgcg gcggggcgcg cacgcgattt ccgggagggc gttgcgatgg cgatggagtc
    18901 gatcgactcg ggagccgcgc gtcgaagaac cgaagatctg gcccgtttca cccagcagtt
    18961 cggctgaccg ccggcgctac caccaggttt tccaaaccag caggcccact ccggcaagga
    19021 agccggccat ggcctgatac tcctgattgc gccagtacag actccagcgg aacggcaagg
    19081 aggacgcaga cgcctggaac cgcggccaga gcatcgggac ccgttccgcg tacccagcgt
    19141 aatccgggaa cagcttccga aggtgttgct cctcgagttc gatcacgggc agatagacga
    19201 gtgcaaaggc ggccgcaagg accgccgcca gtaacgaact gcgcgacgcg agtgcgaatc
    19261 cggcggcgac cagcagagtt ccaagataca gcgggttccg catccaggcg tacggcccgc
    19321 cggtcgcgag gcattcgttc ttggcgaggt ggcccgccgc ccagccgcgt agaagcaaac
    19381 cgagcagagc aattggcaga ccggcggtaa gtgaacgcgg tgccggatcg gagagcagaa
    19441 caaacgccgc cacgagcaga aagccgctcg gaacccggag acgggcgaca gcgtcggcgt
    19501 acggtttcgg gaaccagtgg cgtcgcgtca tcgagcagaa ccagcctggc actgagagac
    19561 ggaaaggcgt tcccggagcg ctgcgagcac gtcgtccggc gagatcctgc ccatcgctgg
    19621 gtggggctcc ttcttccgtt tgtaggacgt ggtggctgcc gggtcccgaa ggacggtgaa
    19681 actggagccg tacgggccat tacgcgaggg gtcagtcgga ccgaagatcg ccaccccggg
    19741 cttttgaagc gcagcggcga ggtgcatcgg accgctgtcg atcccgatta cagcgagggc
    19801 tctgcgcgtg gcgtggatca gcccagagat accggagagg tgaacgactg gtccggctac
    19861 ctgggcaagc gtcccggcag cttgaggcgg gccattgagg acgagcggca ggtcgaggtc
    19921 ccgtcggagg agtccggcga gcgtggaata gtagtcgagc ggccattgct tccccggcca
    19981 tccggcgagc gggttggcga ggacgaaacc gccctccgga agctcgccct cggttgttcc
    20041 atcgggtacc ggaaaggcgc tgacaatact ggacgcaccg gctgccgaag ccagttcgag
    20101 attccggtcc actacgtgcg gggcctgcgc cagcactcgg ttcgagtaaa acaacgcggc
    20161 gacgcgctcc cgcagcagtg attggtggaa tccgtagatc cgatccgggc gggcaacgga
    20221 ggcgacgatg gccgacttca ctaggccctg aaaatcaacg gccagatcga aacggcgatg
    20281 gcggagctcc cgccaggccg aaacaaggcc gctgaacgtc ttccgctcga acaggatcgc
    20341 ctcgtcgacg aacggatttc cctcgatcag cggagcccat ttgggagcga tcacccaact
    20401 cagccgggaa ttagggaagc catgcttcag ggtggcgaca gcgggtaaag cgtgcagcac
    20461 atcacccatc gcgcccaggc gaacgacgag gatcctcggg ccggaattag cttgcgtgct
    20521 gccggctgtg tacatgtcga atcaggttcc gggtgagctg ttcgtcatcg gcttccattc
    20581 gaacggcatc accggcctgg aggcgagcaa gcaggtcacc gatcgtgccg ccatccggaa
    20641 gcaccacgta gtctaccacc gcgagcgccg caaccagttc cgcccgcgcc gcggccgaca
    20701 ggaccggcgc gattggagtg ttcacgacca cgaccaaggc gccggcgccc gctcgcgcag
    20761 cggccaggcg acggccgtgt tccgcgagca ccgggtcaaa gcagccggta acgacgctaa
    20821 cggctacgcc gctgtttcgc agttgccgta cgattgactc ggccgcggcc ggagtgagga
    20881 tcttctttcg tgtgtccact ttcctgtctg gcgaactcct tcgcactttc cgcatccgct
    20941 cccgccgcac gggccagcgt gtaggccgcc catgccgcgg gcgtcttcaa aaaccggtac
    21001 cgttcgtccc gctggtcaag cagcggcatc attcggcggc cgtcggcgat ggcatactgc
    21061 tgctcgacgt cggtgacggc ttcgacggct tcgtcccagt cgcccgcggt gaacagaacc
    21121 gcgtggcagc cgccggccct gtcggcggcg gcctcagcga ccgcctttct ggaccggccg
    21181 accgccgagg catccaaacc aatctgttcg agccggcgtt tcgtagccgc tgagccgagt
    21241 ccgaccggca gcgcccgccc cccaagctgc cgaagcgcct gaagttcagc cgaacttcgg
    21301 ttgaagtcgc cgaccaccag gatcaggaag cgccgcgccc actcgaacat cgtccgcgat
    21361 cccccctatt tcagcaggtc gagggcctcc gccgctaccc gctcagccga gacgcggatc
    21421 atgcaacggt gatcgatcgg gcactcgcgc aagaggcagg ggctgcactc gaccggctcg
    21481 cggatcacac gaacaatggg accggatggg ccggtcgtct cgtagtcggt cgcgccgaac
    21541 accgcgaccg tgggcacacc caacgcagag gcgatgtgca tggcgcccga atcgttcgtg
    21601 aggaacaact cgcacacggc cgccatatcg atgaagccgg cgagcgtcgt ggcgccggcc
    21661 aggttgattg ctcttcgacc tgcgtagcgg acgcctgcct cgacctgctc gcacacgccg
    21721 cgctcgccgg ctgaaccgaa cacggctacg tcagcctcct gctgggaggc gacgcgggct
    21781 gcggcttcag cgaaccgctc cggcaaccag cgtttggctg tgccgtacgc cgctccgggg
    21841 ctgacaccca ccaccggtcc gcgaaggccc agttcttcaa aacgccggcg gccggattgg
    21901 cgcgcggcgg atacggcatc gagcaggatc ggttccgcct ccggcagagc ctccaacatc
    21961 ccagcccgcc taagaagttc gaggtaatag tagcgctcgt ggcgagggat ctctcccgct
    22021 gcaggcggag gcacggcgtg ggtaagcaga atgcgccggg catcccggct gtaaccgatt
    22081 cgcgatggaa tgcgagcgag ccagacaatg agcgcggcgt cgaaggagtt aggcaggagg
    22141 atggcgcaat cgaaactatc gcgcgacagt tcccgcccgg cgttccactt gcttgaccag
    22201 tcccgggcgc cagggctggc ggcatacggg atcacgcgat cggcgaacga ctcccggaag
    22261 tagagatcgg ccacccaagg acgcgcctgt acggcaatgt gcgccttagg gaagcgctcc
    22321 cgcagagccc gaagcgccgg cagcgacatg acggcatcgc ccacccagtt ggtggaccgc
    22381 acgaggatcc gctgaaaatc gggagtcgcg gccaagtctt catggtacag cctggagccg
    22441 agacccagcg gaccgctaca atagcagcag gctgcctcgg aagccgaaat gggacgattc
    22501 tacactcgag aagaactggt ggccgcacgg gccgaatgga agcggcaggg taagtccgtc
    22561 gtctttacaa acgggtgcta cgacatcctg catccggggc acatacgcct gcttgagaag
    22621 gcccgggccc tgggcgacat cctgattctg gcgattaaca gcgactctag cgtacgccgg
    22681 ctcaagggcg cgtcccggcc gttgaacccc gaacaggaga gggcggaact cgtgctcgct
    22741 cttcaagcag tcgacgccgt gaccgtgttc gacgaagaca ctccccgaga gttgatcgcc
    22801 gaggtgttgc cggacgtgct gatcaagggc gccgactggg cgcatttcat tgcaggtcga
    22861 gaagaagtcg aagcggcggg cggcaaagtc ctttcgacgc cgcttgagcc tggatattct
    22921 actactggaa tagtagaact gctcctgacg cttaccgttc agcccgaatg actcatccgg
    22981 agattcgtaa cctcctggaa ggattgggcc ggcatcccgc ctttcaggaa ctgttgggcc
    23041 ggatacaaaa ggaaccggtc tcgcgcaacc ccctctccgg cctgaaccca gcagcaaaag
    23101 cactctacac ggttctcctg tgcaaggcgt ttgagcggcc ggtgatcgtt gtcgtcgaag
    23161 gcgtccggca agcggaggcc cttggtgaaa ctgtcgagac tctattcgac ctgcttcgtg
    23221 gtggacgctc cgtcccgccg ccacaagtcc tgccggcgct ggacgttctt ccgcatcaac
    23281 ggttgtcccc gcactcggag atcagcgaag agcgtgcggt cggtctctgg cggctcgcct
    23341 cgggcgtggc cccgatcaca atcaccccga tcacgtcggc gctgcttcga accgagccgg
    23401 ctgagtttta ccgccaactc gcgatcacgc tccgggccgg agaagaggtc ccgttcgacg
    23461 cggttgtaga gcacctgcgt tcggtagggt acgagcaccg ggagccggtc gagatggtcg
    23521 gggagttctc agtccgcggc ggcattctcg acgtgttctc gcctgaggcg tcccggccgg
    23581 tacgcatcga attcatcggt gaccaggtgg aatcgatgcg gagattcgac gtggagacac
    23641 agcgatccgt ggttaagacc gaggagtgca cgctgctgcc tctatcggag acgccgaagt
    23701 cggccggtct tttcgccgag ctctacgaac acctggggcg ggagcacgtg ccgaacgtgg
    23761 acgaggcgtt ccccggttgg gagttcatgg cggcggccgt acgtccccgc gagaactcgc
    23821 ttatcgaact ggcggagaaa cccctagtga tatgggacga gccggctcag ctcagggatg
    23881 tcgcaaaccg tctatgggaa cgcatggaga cgaccgaacc ggtcggcacc tgtcctccgg
    23941 accggatctt cttccggtgg aacgaattgg gcgaaaggct cggctcggcg gcacaggttg
    24001 agttcaccga gcttgcgctc gatcctcgac ccggcccgca catcggcacc cggccggcga
    24061 tgacgttcca tggcaatatg cccgtagcgg tcgccgaaac caggaatgcg gtcgagcagg
    24121 gcagccgtgt ggtgttcttc gccgcatcgg caggagaact ggagagactt gccgacgtct
    24181 tccgggaata ttcggttccg tttcagcttg gcattgagcc ttccgcctct acgccccagt
    24241 acctggctga gcacgcctat ctcgcgggct ctgcggcaag cacgtatctg gtcaagggcc
    24301 ttgtccggcg cggagtggtg ttccccgagc agcaggtgac attgtttggc tcggaagatc
    24361 tgttcgattc ctcggaactg gttccgcggc cggcggcgcg cgcacaccat ttcgcgttct
    24421 cgctcgacct caccgacctg aaacctggcg attacatggt tcactcggag cacggagtcg
    24481 gccgtttcct ggggctgcgg gagctctccc tcggagatca aaagggcgac ttcatgctgc
    24541 tcgagtacgc cggcgaggcc aaactgtacg taccgcttcc gcggctcgat ctggttcaga
    24601 agtatcgcgg ggcgggcgac ggagcgccgc cgctcgaccg tctcggcgga acgacctgga
    24661 accgcacgaa gagccgggtg aaggcgaaga tgcgcgacat ggctgacgag cttctcaagc
    24721 tttacgccga gcgcaaacta gcgcagggtg tggccttctc ccccgacagc aactggcagc
    24781 gcgagttcga ggacgcgttc gagttcagcg agacgaagga tcagctcgtg gccgtccagg
    24841 aaattaagcg tgacatggag gtggcgcatc cgatggaccg tctcctctgc ggcgacgtcg
    24901 gattcggcaa gaccgaagtc gccatgcggg cggtgtttaa ggtgctgggc gacggccggc
    24961 aagcggccgt tctcgcgccg acgacggtcc tctgttttca gcactacgag accttccgcc
    25021 gccgtttcgg tccgtttccg gtccgcgtcg agatgctaag ccgttttctg tcgcccaaag
    25081 agcgaaagac cgtccttgcc gacctcgcgg acggaaaggt tgacatcgtg atcggcacgc
    25141 accggttgtt gtcgaaggac gtacagttcc gcgatctggg gttgctgatc gtggacgaag
    25201 aacagcgctt cggcgtcggg cacaaggagc gcctcaaaca gattcggaaa aacgtcgacg
    25261 tgctgacgat gagcgccacg cccatcccgc gaacgctgca catgtccctt gtcggactgc
    25321 gcgacatgtc cgtcatcgag acgcccccga aggaccggct ggcgatccac accgtcgtag
    25381 cgcatttcga ctgggacctg atccgtaccg ccgtcgacca ggagttgagc cgcggcggcc
    25441 aggtctactt tgttcacaac cggaccgaca gcatttggtc gcgggcggcg atgatccagg
    25501 agctgacgcc ccattgccgg gtcgctgtcg gccacggcca aatgagcgag gggcaactcg
    25561 agaaggtcat gctcgggttc atgaagcacg agtatgacgt gctcgtatgt acgacaatta
    25621 tagaaaatgg gcttgatatc cctcttgcaa atacgattat cgtagatcac gccgagcgtt
    25681 acggcctctc cgagttgtat cagctccggg gccgagtagg ccgttcgaat cgaagggctt
    25741 acgcttatct cttggtaccg ccggacactg aactgtcgga gaccgctcga aagcgtttgg
    25801 cggcgctgaa agaattcagc gaactgggcg ccgggttcaa gatcgccgca ctcgacctgg
    25861 agttgcgtgg ggccggtaac cttctcggcg gcgagcagca cggccatatt aacgccgtcg
    25921 gatacgacgt gtacgtgaaa ctgatggagg agacggttcg cgagttgaag ggagaagagg
    25981 ttccggtcga ggtccattcg acattgaacc ttggactcga cgtccggata ccccccgagt
    26041 acatcgcgga ggagcaccag cggctccgcg tctacaagaa gatcgccgat gtcggcgagc
    26101 gtagccaggc ggacgcgatc ctggccgaac tccgggatcg ctttggcccg ccgcccgagg
    26161 cggtgtcgaa cctgctcgag ttctcgctcc taaaatcact cgccaaacgg cttggcattg
    26221 agtcgatcga gaggcgccag ggcgccctca acgtgaagtt ccaccgggaa tcccgcgtag
    26281 acccggcgcg gctgatgaac ctggtgagcc gcacggtggg tgggcaattc acgcccgcgg
    26341 ggattctccg tgtgcccctc gaaggcgatt ccaccccgcg gaaggtgttg gaacgggtga
    26401 aggactgcct cgtgcatctc tcgccgtagc tatttcataa tagagctatg gtgtcagtgt
    26461 tgtgttttct cggtacattc gtgctcgctt ggggcgcttt cgcctcagac gtgcgggtcg
    26521 tcgaagtgat cgtggcgaag gtaaacaacg agatcatcac atcgagcgaa ctcgaacgcg
    26581 ggcgccgtct gatcgaagcc cagttgcgcc gaagcgggac gggcggccag caactcgagg
    26641 aggccgtgaa ggagcgtaca aaggacgtgc tgcgcgaccg catcgatcag ttgctgctcg
    26701 tgcagaaggg caaggatctg gagatcaacg tcgacagcga ggtgacgaag gaacttgcca
    26761 acatccaact gcaaaacaag attgccgatc aggacaagtt ccagcagtgg attcgcgaac
    26821 agagcggcat gccgtttgag gacttcaagg cggaaatgcg aaac
//
LOCUS       DATIFQ010000325         6751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_299304, whole genome shotgun sequence.
ACCESSION   DATIFQ010000325 DATIFQ010000000
VERSION     DATIFQ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299304"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3064)
                     /gene="treS"
                     /locus_tag="VLE22_15145"
     CDS             complement(<1..3064)
                     /gene="treS"
                     /locus_tag="VLE22_15145"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HSB15793.1"
                     /translation="MKLPGSAQDPLWYKDAIVYEAHVKAFFDADNDGIGDFSGLLHKL
                     DYLQNLGVTCLWLLPFFPSPLRDDGYDIADYSNVHPSYGTLDDFKIFIAEAHQRGLQV
                     IIELVVNHTSDQHPWFQAARKAPPGSPERDFYVWSDTDQKYHDARIIFLDTEKSNWTW
                     DTEANAYYWHRFFSHQPDLNFDNPLVVQEIVKVMEFWLDMGVDGLRLDAIPYLVERDG
                     TNCENLAETHAVIKRFRKEMDSRYADRMFLAEANQWPTDVRPYFGDGDECHMAFHFPL
                     MPRIFMALRLEDRHPITDIMAQTPEIPGNCQWGLFLRNHDELTLEMVADDERDYMYFA
                     YSADPRMRLNLGIRRRLAPLMDNNRRRVELLNGLLFSFPGTPIIYYGDEIGMGDNIYL
                     GDRNGVRTPMQWSADRNAGFSRCFPARLYSPVIMDPVYGYEAVNVEAQQSDPSSLLNW
                     MRNMIALRKLFKVFGRGTVEFLHPANRKVLAYLRRHEHDRILCVANLSRFAQAVTLDL
                     SSLAGMTPVEMLGYSEFPAIGVEPYPLTLGPYGFYWFELQGAPTPADVTTKATLEEHA
                     PLVLEGTWEDLLEGHATQSLTTRVLPRFLPGQRWFAGKARTITATRVRDWARIVEGPS
                     CPSVLALIDVDFADGGTDSYLLPLFLVMGREAEQLLDCQPGAVLCRIADSGKTGLLCD
                     ATADDTTCQGLLTAIERGQEFIGRKGRIRAFASHAYSYARGADTGPLPVVRSSAEQSN
                     TSVIYGDRMILKFFRRLEPGPNPDFELGRYLTEETHFSRIPRLAGAIEYVPHGSGPST
                     LAMLQALVENQGDGWRWTLEELGRYFEQCATALSSVPPPHNCALSLFDLSETEAPAEA
                     RESIGIYLDASAVLGRRTAEMHLALGAATSDPAMIPEPMTGDDLSQLAGELRNHATKV
                     FELLKAGLALLPEEIVDKAGLALSMRRRLLTRFEGLAVIRTGGERIRVHGDYHLGQVL
                     RAMNDFVILDFEGEPARPVAERRAKQSPLKDVAGMLRSFSYAAHAGLQS"
     gene            complement(3075..4973)
                     /gene="glgB"
                     /locus_tag="VLE22_15150"
     CDS             complement(3075..4973)
                     /gene="glgB"
                     /locus_tag="VLE22_15150"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HSB15794.1"
                     /translation="MSSTFLTDFDLHLLAEGTHYRSYSKLGAQLWRHEERDGAHFAVW
                     APNAESVSVIGNFNGWDPDANPMRFRPDAGLWECFVPGIGHGALYKYHVASKYNGYRT
                     ERADPYGFAAEMRPQTASKVWDLSGYQWGDGEWMRGRAQRNSSSAPIAAYEVHLGSWK
                     RVREENNRWMTYREMAPRLADYVGEMGFTHVEFLPVMEHPFDGSWGYQTVGYFAPTSR
                     FGTPDDFMYLVDTLHQHGIGVFMDWVPGHFPTDGHGLAFFDGTHLYEHADPRQGKHPD
                     WDTFVFNYGRTEVRNFLISNAVFWLEKYHIDGLRVDAVASMLYLDYGRRDGEWMPNKF
                     GGRENLEAIDFLRTLNEVVYGQFPDVVMVAEDSTAWPMVSQPTYLGGLGFGLKWNMGW
                     MHDMLDYMSKDPIHRSFHHNRITFSLLYAFNENFVLPFSHDEVVHGKSSMIGKMPGDD
                     WQKFANLRVLYGYMIGHPGKKLLFMGGEFGQWREWNHDEDLDWPLIGYPQHRGLQRWV
                     RDINTFYRSEPALHQLDCDPGGFEWIDCSDTQRSVVSFLRKGRDPLDTVAFVCNFTPM
                     PRHNYRIGTPTGGDWREVLNSDATLYGGSGQGNLGGAVATPVGAHGRPFSLNITVPPL
                     AVVAFRRN"
     gene            complement(4989..>6751)
                     /locus_tag="VLE22_15155"
     CDS             complement(4989..>6751)
                     /locus_tag="VLE22_15155"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HSB15795.1"
                     /translation="EFTVGQMGRYYYTVEGWLDQFQTWRHDFIKRIQAAQEVEVDLLI
                     GASLVSAASQKAEGADAERLRQWAAELSGKKSAEFRAQLALDEELGKLASHYADRRLA
                     TRYPHELPVTVERPVARFGAWYEMFPRSCSPELGRHGTFRDCEALLDYVGGMGFDILY
                     LPPIHPIGRTNRKGKNNTTFCGPDDVGSPWAIGAKEGGHKSIHPQLGSMEDFEHLISR
                     AGEYEIEIALDLAFQCATDHPYVKECPEWFRARPDGTIQYAENPPKKYQDIYPFDFES
                     SEWRGLWEELRSVVTFWIEKGVHIFRVDNPHTKAFRFWEWLIADVKKEYPQVIFLSEA
                     FTRPKVMYRLAKLGFSQSYTYFTWRNTKRELTEYFEELAQSPVREFLRPNLWPNTPDI
                     LPEYLQMGSRPAFVSRLVLAATLGASYGIYGPAFELCENEPKEPGSEEYLNSEKYELK
                     HRNLDSPWSLKDFIARVNRIRRENQSLHHDWRLHFHAVDNDMVICYSKSTPDFSNVIL
                     VVVNLDFRHTQSGWVTLDLDALGLRESEPYQVHELLGEGRFLWQGMRNYVELDPQQAP
                     AHIFRIRRKTRTERDFDYYL"
BASE COUNT         1367 a         2035 c         1881 g         1468 t
ORIGIN      
        1 agctttgcag accggcgtgc gccgcgtagc tgaacgaccg caacataccc gccacgtcct
       61 tcagtggcga ctgtttcgcc cgccgctcag cgactggccg cgctggctcc ccctcgaagt
      121 cgaggatgac gaaatcattc atcgcgcgga gcacctgccc aaggtggtaa tcgccgtgga
      181 ccctgattct ttccccgccg gttctgataa ccgccagccc ttcaaaacgc gtgaggagcc
      241 ggcgccgcat gctcagggcc agtccggctt tgtccacgat ttcttccggg agcaatgcca
      301 gacccgcttt cagcagttcg aagactttcg tggcatggtt ccgcaattcg ccggcgagct
      361 gcgacagatc gtcgccggtc attggctctg ggatcatcgc aggatcgctg gtggcagctc
      421 cgagtgcgag gtgcatctcc gcggttctcc tgccgaggac agcggacgca tccaggtaga
      481 tgccgatgct ttcacgggct tccgcgggtg cctcagtttc cgagagatca aacagcgaaa
      541 gcgcacagtt gtggggcggc ggaaccgagg acaacgcggt tgcgcattgc tcgaaatagc
      601 gcccgagctc ctcgagggtc catcgccatc cgtcgccctg gttctccacc agtgcttgaa
      661 gcatggccag agtggatggc cccgagccgt gcggtacata ctcgatggcg ccggccagcc
      721 tcggtatccg gctaaagtga gtctcctccg tgaggtagcg gcctagttcg aagtcagggt
      781 ttggccccgg ctccaggcgc cggaagaact tgaggatcat ccgatctccg taaatcacag
      841 acgtgttgct ttgctcggcg gaactgcgta ccactggcag cgggcctgta tccgcgccgc
      901 gagcgtacga gtaagcgtgg ctggcaaagg cgcgaatccg tccctttcgg ccaatgaact
      961 cctgtccgcg ctcgatcgcc gttaggagtc cctggcatgt tgtatcgtcc gcggtggcgt
     1021 cacacaacag gcctgtcttt cccgaatcgg cgatgcggca taacacggcg cccggctggc
     1081 aatccagcaa ttgctccgcc tcgcgaccca tcaccaggaa gagcggcaga aggtacgaat
     1141 ccgttccacc gtcggcgaaa tccacgtcga tcagggcaag gaccgacgga cacgacggcc
     1201 cttccacaat ccgcgcccag tctctcacac gcgtcgccgt gatcgtacgc gcctttcctg
     1261 cgaaccagcg ctggccgggg aggaatcttg gcaggaccct ggtcgtcagg ctctgtgtgg
     1321 cgtgtccctc caacaaatct tcccaggtcc cctccagaac gagtggggcg tgttcttcca
     1381 gtgtggcctt cgtcgtaacg tcagccggtg tcggtgctcc ctgcaactca aaccagtaga
     1441 agccgtaggg gcccaacgtt agcgggtatg gctcaacccc gattgccggg aactcgctgt
     1501 atccgagcat ctcgaccggt gtcatcccag ccagtgaaga caagtccaga gtaaccgcct
     1561 gcgcgaatcg ggaaaggttg gctacgcaca agattcggtc gtgctcatgg cggcgcagat
     1621 aggccagcac tttccggttg gccgggtgca ggaattcgac ggtgcctctt ccgaacacct
     1681 tgaacaactt ccgcaaggcg atcatgttgc gcatccagtt cagcagtgac gagggatcgc
     1741 tctgctgcgc ctcgacgttc actgcctcat aaccgtacac aggatccatg atgacggggc
     1801 tgtaaagtct cgccggaaag caccgtgaga acccagcgtt cctgtcggcg ctccactgca
     1861 tgggagtgcg cacaccattc cgatctccaa ggtagatgtt gtcacccatg ccaatctcgt
     1921 caccgtaata gatgatcggc gtgcccggga aggagaagag caggccgttg agcagttcaa
     1981 cgcgccgccg gttattgtcc atcagcggcg ccagccgccg gcggataccg aggttcagcc
     2041 gcatccgcgg atctgcgctg taggcgaaat acatgtagtc gcgctcgtca tctgccacca
     2101 tctccaacgt cagttcgtcg tggttgcgga ggaacagtcc ccactggcag ttccccggaa
     2161 tctccggtgt ctgagccatg atgtcggtaa tcggatgacg gtcttcgagc cgcagagcca
     2221 tgaagattcg aggcatcagc gggaagtgaa aagccatgtg gcactcatcg ccgtccccga
     2281 agtacggccg gacatcggtg ggccactgat tggcttccgc caaaaacatc cggtcggcat
     2341 agcgggaatc catttctttc cggaaacgct tgatcaccgc gtgtgtctcg gcgaggttct
     2401 cgcagttggt gccgtcacgt tccaccaggt aaggaatggc atcgagccgc agcccgtcaa
     2461 cacccatatc cagccagaac tccattactt tgactatctc ttgcactaca aggggattgt
     2521 cgaaattcag atccggctga tgagagaaga agcggtgcca atagtacgca ttcgcctcgg
     2581 tgtcccaggt ccagttcgat ttctccgtgt ccaggaagat gatccttgcg tcgtggtatt
     2641 tctgatcggt atcgctccag acatagaaat cccgctccgg tgaaccgggc ggcgccttcc
     2701 gagctgcctg aaaccatgga tgctggtcgg aagtgtggtt taccaccagc tcgatgatga
     2761 cttgaagacc tcgctggtgt gcctctgcaa tgaaaatttt gaaatcatcc aacgtcccgt
     2821 agctgggatg aacgttggag tagtcggcga tatcgtatcc gtcgtcgcga agcggagacg
     2881 gaaagaacgg caacagccac aaacacgtaa cacccaggtt ctggaggtag tcgagtttgt
     2941 gtaggagccc ggaaaaatcg ccgattccgt cattatcggc gtcgaagaac gccttgacgt
     3001 gcgcttcgta gacaatcgcg tctttgtacc agagaggatc ctgtgcgctg cctgggagtt
     3061 tcattgcttc ccgctcaatt tcgcctgaat gctacaaccg ccagcggcgg gactgtaatg
     3121 ttcaatgaaa agggcctgcc gtgggcgccc acaggagttg ccacggcgcc gcctaggtta
     3181 ccctgtccac ttcccccgta tagtgttgca tcgctgttca gaacctcgcg ccaatctcct
     3241 cctgtgggcg tgccgattcg gtagttatgt cgcggcatgg gcgtaaagtt gcacacgaat
     3301 gctacagtgt ccagcggatc cctgcctttc cgaaggaaac tcacgacact ccgctgcgtg
     3361 tcgctgcagt cgatccattc gaaaccgcct ggatcgcaat cgagctgatg cagggcaggt
     3421 tcgctccggt agaacgtgtt gatgtcacgc acccagcgct gcaaaccacg gtgctgcgga
     3481 tacccgataa ggggccagtc aagatcttcg tcatgattcc attcgcgcca ctgcccgaac
     3541 tcaccgccca tgaagagcag tttcttcccc gggtggccga tcatgtagcc gtatagaacc
     3601 ctgaggttgg cgaacttctg ccaatcgtca cccggcattt tcccgatcat cgaactcttg
     3661 ccgtgcacca cctcgtcgtg tgagaaaggc aacacgaaat tctcgttgaa cgcgtaaagc
     3721 aggctgaacg tgattcgatt atggtgaaac gagcggtgga tgggatcctt cgacatgtag
     3781 tccagcatgt cgtgcatcca gcccatattc catttcaggc caaagccgag tcctcccagg
     3841 taagtgggct gtgagaccat cggccacgcg gtggagtctt ccgctaccat gacaacgtcc
     3901 gggaattgcc cgtagacgac ttcgttcagc gtgcgcagga agtcaatcgc ctccaggttc
     3961 tctctgcctc cgaacttgtt cggcatccac tcgccatcac gcctgccgta gtccaggtaa
     4021 agcatgcttg caaccgcgtc aacccgcaaa ccgtcgatat gatacttttc gagccagaat
     4081 accgcgttgc tgatcagaaa attccgcact tccgtcctgc cgtagttgaa tacgaaagtg
     4141 tcccaatccg gatgttttcc ctggcgcggg tcagcatgtt cgtagagatg cgttccgtcg
     4201 aagaatgcca gaccgtgccc gtcggtcgga aagtgcccgg gcacccagtc catgaatacc
     4261 ccgatcccat gctgatggag cgtgtccact aggtacatga agtcgtccgg cgtaccgaac
     4321 cggctggtcg gagcgaagta gccgactgtc tggtagcccc aggatccgtc gaacggatgc
     4381 tccatgaccg gcaggaattc tacatgggtg aagcccattt ctccgacgta atcagccagt
     4441 cgcggagcca tctcccgata cgtcatccag cgattgtttt cttcgcgcac gcgcttccac
     4501 gagccaaggt gcacctcgta agcggcaatt ggggcgcttg acgagttgcg ctgggctctt
     4561 cctcgcatcc attcgccgtc tccccactgg tagccggaaa gatcccaaac ctttgaggcc
     4621 gtctgcggcc gcatctctgc ggcgaatcca tatgggtcgg ccctttccgt gcggtacccg
     4681 ttatacttcg acgcaacgtg atacttgtac aatgcaccgt gtccgattcc gggtacaaag
     4741 cattcccaca agcctgcgtc cgggcggaat cgcattgggt tcgcgtcggg atcccaaccg
     4801 ttgaagttcc cgatcaccga gacgctttcc gcattaggcg cccacaccgc aaagtgggcg
     4861 ccatcccgct cctcgtgacg ccacaattgg gcgcccaatt tgctgtagct gcggtagtgc
     4921 gtgccctccg ccaacagatg caggtcgaaa tcggttaaga atgtgctgct cacgcgacag
     4981 cctcctttct acaggtagta gtcaaaatca cgttccgtcc gcgtcttccg ccggattcga
     5041 aatatgtgcg ccggtgcctg ctggggatcc aattccacgt agttgcgcat gccttgccat
     5101 agaaagcgcc cttccccgag cagttcatgt acttggtacg gctcgctctc gcgaagcccc
     5161 agtgcatcca ggtctaacgt cacccagccg ctctgtgtgt gtcggaagtc caagttcact
     5221 acaacgagaa tcacgtttga gaaatcaggc gtgctcttgc tatagcagat caccatgtcg
     5281 ttatctacgg catgaaagtg cagcctccag tcatggtgta gcgactgatt ctccctccgg
     5341 atcctgttca cccgtgcaat gaaatccttg aggctccacg gcgagtcgag gtttcggtgc
     5401 ttgagttcgt acttctccga gttcaagtat tcttcgcttc cgggttcctt cggctcgttc
     5461 tcgcataact caaacgccgg accgtagatg ccataactgg cccccagcgt agccgccaga
     5521 acgagtcgtg acacgaatgc tggacggctg cccatctgca ggtactccgg caggatgtcc
     5581 ggcgtattcg gccataggtt gggccgtaag aattctcgca ccgggctctg tgcaagctcc
     5641 tcaaagtact cggttagttc gcgcttggtg tttctccagg tgaaatatgt gtatgactgc
     5701 gaaaagccga gcttggcgag tcgatacatc acctttggcc gcgtaaacgc ttccgataga
     5761 aaaataacct gtggatactc cttctttacg tcagctatga gccactccca aaatcggaat
     5821 gctttggtgt gcggattgtc aacccggaag atatgaacgc ccttctcgat ccagaacgtg
     5881 accacactgc gtaactcttc ccaaaggcct cgccactcgc tgctttcgaa atcgaatggg
     5941 taaatgtctt gatatttctt cggtgggttc tcggcgtatt gaatcgttcc gtccggcctc
     6001 gcgcggaacc actcgggaca ttctttcacg tacggatggt cggtcgcgca ctgaaaagcg
     6061 agatctagag cgatctcgat ttcgtattct ccagcccgcg aaataagatg ctcgaaatct
     6121 tccatggagc caagttgcgg gtgaattgac ttgtggccgc cttccttagc gccgatggcc
     6181 cacgggcttc ctacgtcgtc cggcccacag aacgttgtat tgttctttcc tttccggtta
     6241 gtacggccga tcggatgaat tggcggaagg tacagaatgt cgaaacccat gcctccaacg
     6301 tagtcgagca gcgcctcaca gtcgcggaac gtgccgtgtc ggccaagctc gggtgagcag
     6361 gagcgaggga acatctcata ccaggcgccg aagcgggcca ctggccgctc gactgttacc
     6421 ggcagttcgt gtgggtaacg tgtggcgagc cggcgatccg cgtagtgtga cgccagtttc
     6481 ccaagttcct cgtcaagagc tagctgtgcc cggaattcgg cgctcttttt gccagacaac
     6541 tcggccgccc actgtcgcag cctttcggcg tccgcaccct cagccttctg ggatgcggca
     6601 gaaaccaggg atgcgccgat caatagatcg acctcgacct cctgcgcggc ctgtatccgc
     6661 ttgatgaagt cgtggcgcca agtctgaaat tgatcgagcc agccttcaac ggtgtagtaa
     6721 tagcgtccca tctgacctac cgtgaattcg g
//
LOCUS       DATIFQ010000326        11131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_299351, whole genome shotgun sequence.
ACCESSION   DATIFQ010000326 DATIFQ010000000
VERSION     DATIFQ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11131
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299351"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VLE22_15160"
     CDS             <1..258
                     /locus_tag="VLE22_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15796.1"
                     /translation="RDTGMDQIVEAHCLGRIVQYGDSADLREALAEFASWSPEAWEEF
                     SRHARGVYSAVFSWSVMKSRLQTLYSTLPARVGGRGRLATA"
     gene            255..1418
                     /locus_tag="VLE22_15165"
     CDS             255..1418
                     /locus_tag="VLE22_15165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB15797.1"
                     /translation="MRIYKPAFFWFVVALAVRAAAALALHLYSISAGFEGFYPLASGH
                     DDRQYWKLAASLADGMKPVDLPHAYPLVLAFLFRCFGPSLVLGKALNILAGSVTVALG
                     VLITRDLSRHVTTSWRKTLHPVNVAGLLLTFYPSAVFYSTQLVKDPILVLLGMWSVYI
                     VVRIVHERRFSLWALWVGSLAALYLFRPYAACVMGLSVLVFLTSFWRARLRTRVTVLL
                     IAVLTSASVPNLLGWGWFGSSYLAPLLDPEFVIQFRERAYSSGGSAVGIELNYLEPLS
                     FVSSYSYGLLTVLFGPFPWQIRSAVMYIALPESLVMLSLGIVALSKKSKPDVYQREQL
                     LLLILAVLMSAAIALFSDNIGANTRLRLLPWSALLIYGAAKWDSRRRTSVYST"
     gene            1379..2488
                     /locus_tag="VLE22_15170"
     CDS             1379..2488
                     /locus_tag="VLE22_15170"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB15798.1"
                     /translation="MGQSSKNLRVLYLITKSEAGGAQSHVLSLLRDLRSEFDFALGVG
                     EEGQLSERARELGISVFRLRYLVHRPDPLRDPLAVGEVLGLIRRWQPTLLHAHTAKAG
                     LVGRAAARLAGIRSVYTPHGWAFSERAGRLQRVAAIRCERWLSNWDAEIITVSGAERA
                     VAERAGLSMNGHCHVIHNGVPDTPHRAGPRSYESGVMIHVGRFVAEKDHDLLLRSLSQ
                     LRCRYRLVLVGDGPLRSRVEAQAVALGLSRNIDFLGTRDDIAELLSQASLFVLPSKWE
                     AFPISVLEAMRGGLAVVATNVGGLAEAVDDGETGFLVPRGDAVSLAQRLSRLLESPAS
                     AATMGVQGRARYERLFTADLMLNRTLEIYRRALMG"
     gene            2543..3967
                     /gene="wbaP"
                     /locus_tag="VLE22_15175"
     CDS             2543..3967
                     /gene="wbaP"
                     /locus_tag="VLE22_15175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HSB15799.1"
                     /translation="MTRVRAHGQPVARPALMVSSLVLTDLTALCVAGGLSVLGRYLFG
                     GQFTFWLYFDMWPLLLSFPVSFAAIDLYDEVGISPPEELRRTTMAMSLVYLVIGAGTF
                     LLRGAEDYSRAVFLVAWMLSLVFVPIARHTVRNNLCRRPWWGRPAVAIVKGRRGELVL
                     SSLLSQASLGLKIIGVVTDDKSTAHSICGIPVIGDLTSLPSVSSSTGVRHGIIAMPAA
                     SRRRLADVVEACRQLLANVIVIPEWFDPSRPWVASRDINGVPGLALRQELLLPGPRLI
                     KRCLDIIGTIVLSCVFTPLLVVTALSVVICSPGKVFYGHRRVGQGGRRFNAWKFRTMV
                     RDGDRMLRRCLAESPALRKEWEQAHKLRNDPRVIRVGHFLRRSSFDELPQLWNVLRGE
                     MSLVGPRPIVDDEIRRYGEHFDLYKAVRPGITGLWQVSGRNDTTYAERTNLDRYYVRN
                     WSVWLDLYILARTVRAMLLGRGAY"
     gene            4011..4100
                     /locus_tag="VLE22_15180"
     tRNA            4011..4100
                     /locus_tag="VLE22_15180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4045..4047,aa:Ser,seq:tga)
     gene            complement(4106..5548)
                     /locus_tag="VLE22_15185"
     CDS             complement(4106..5548)
                     /locus_tag="VLE22_15185"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HSB15800.1"
                     /translation="MSVRLSDGREIPIEMHKVRIVQKANLIPAGQRLAAMEEAGYNTF
                     LLRTKDIFLDMLTDSGTNSMSDNQLAAMMTADDAYAGSESFYKLAAAVKDVFGHEYLL
                     PAHQGRAAEHLLAKVFVKPGDVVPMNYHFTTTRAHFEIAGGTVLEIFTDEALVTQSTC
                     PFKGNLDIDKFRKVIAQYGADKISFVRMEATTNLLGGQPFSLGNLRAVKEVAAQNGIF
                     LVVDGSLISENAYFIKQREEGYQDKSILEIIREIMNLADVCYLSGRKSCSARGGFIST
                     SRKDLMDRIRPWLPVYEGFFTYGGMSTKEVEAMAVGVREMVETEVAGSSAEFVKYFVN
                     RLSENGVPVVTPAGGLACHVDAHKFLPDIPSLGYPAGALAAAVYVCSGIRTMERGTLS
                     MDRDREGNEVPSDLELVRFAVPRRVYTMSQIEYAVDRVTWLHRNRNLIKGLKFVEEPP
                     VLRFFVGRLIPLESWAAKLVDAYRSDFGEN"
     gene            5684..7702
                     /locus_tag="VLE22_15190"
     CDS             5684..7702
                     /locus_tag="VLE22_15190"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HSB15801.1"
                     /translation="MPAAPETASVSLPENAYKPLAPGETYRPVVPAAEAPPELTGRSI
                     GWGVLLCVIFTVASAYSGLKVGQVMEAAIPISILAIGLARMYPRRSSVLENVIITGIG
                     GVAGSVVAGAIFTLPALYILKLDPHPVQTIFICLAGGCLGTLFLIPLRRYFVRDMHGL
                     LPYPEATAITEVLITGEKGGSQARLLLQATAIAGVYDFFVTTFQVWKEFVDFHFLPIM
                     RTLSDRAKIVFNFDAIGFILGLGYVMGLRSSMILCAGGVLSNFVLVPLIWMIGSHLPD
                     LAVYPAKNPISQMDAGQIFRGYVRFIGVGAIATAGIFGIVKSLRVVAGSFAIAARAFR
                     RGEAGGMERTDRDMSVVSILAGVVVSAVAVAIFLGSLSSSVTVIAIGLLLTLAFSFFF
                     TSVAANAIATIARNPVSGMTMLTIIISSVVLLRFGLSGTTGMFFVMAIAGMVCTALSV
                     SGQAITDFKAGYWLGSTPAAQQKVKFLGVIAAAAAAGLTIVLLARAFQFGEAAPGDPR
                     TVLPSPQAAIMKALVEGFVGRQPVASILFGAGAFVALIMEMLGVPALIFALGMYLPLE
                     LNTPALVGGFLAHFLGRRAEKAGGGRGASIRERGVIIASGLMAGGALGGVLGAAFRLI
                     PGYSESWIKTPFYDFDPVSQFVSAGLFAGLCLYVWFGSLRSSGREVNS"
     gene            7699..9039
                     /locus_tag="VLE22_15195"
     CDS             7699..9039
                     /locus_tag="VLE22_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15802.1"
                     /translation="MSARFEEVAGNAILGIDTLWGGDVMCPSGTGRFIADSWFSDEPL
                     PHAYTHPAAALVRESGGVSAKTLDREAIEAYLDAVDVPAAIRGMGEAGKAMGGLRGAY
                     VSNLALCFQTMWDLVLELLGKGEPVPYARCVHASIGADPSPSDPVSKRERVAELLSRC
                     GHPTRTPDELLAAVDSWRRARMVPMASVRALGAAFIAQFDRLAAENLIPYLPPELHGV
                     PRANIQFLPITGAWFSGSMNYLGRARRSDGTPDYEATYEINASLEISVPEFEQLVSHE
                     VVPGHVTTFAYLQNLYVRSLIGFEGSVLTMNTRSAALFEGIANNAILMAHGVTEIDQV
                     PDDDLQIGLLLALLQDDAKNQSSYLTWKEGAPEAEVAAVLRKDFLVSVERADKLSGAW
                     GRHPLLGRMYLPAYRAGTDRIAELRRVHSAKKLLPVLYGCAGLVDLTTIDSALA"
     gene            9090..9635
                     /locus_tag="VLE22_15200"
     CDS             9090..9635
                     /locus_tag="VLE22_15200"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HSB15803.1"
                     /translation="MSFLRLFAAQTLLAASLLGASSIHEFTLNSIDGKAAPLSEYKGK
                     VVLLVNVASRCGFTPQYAGLEALYRKYKDRGLVILGFPANNFGSQEPGTNEEIKQFCS
                     RKYDVTFPMYAKISVKGDDQAALYQFLTDKSAHPDTGGEIQWNFTKFLAGRDGKILAR
                     FEPKVTPESPELTSAIEKALE"
     gene            complement(9683..>11131)
                     /locus_tag="VLE22_15205"
     CDS             complement(9683..>11131)
                     /locus_tag="VLE22_15205"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSB15804.1"
                     /translation="IYTGNMLIDCDGEVPHNETSIAVNPNDPKHVVGAYHSYQVKFRG
                     ATAIAPVIGTVSVSTDGGENWREVVPPITPYQFTGDPALSFNADGRLYFGNIADHEGP
                     GGSYTSPSVVVAHSDDGGFTWSNPVAVATGLGAVTKGRIARQVFQDKDFIAADAYAGS
                     PYKNRVYVTWTSFQENPRGVAPFFRSPIQVSLSDDGNRWSGATEISGYSPFCIATLSG
                     APNECDLNQYSIPAIAPTGKVYVAFENFNTPAENQYMVVNSTDGGQTWGAPSRVDTVY
                     DINYPEGVSGDSVLTGCEFRVNAAGNIATDPSDASGDTAYLVWADNRNGSEEATNVDV
                     FLGRTTDGGATWIIYPVDTDSNDQFYPWVAVAPDGRVDVGYMDRSYSEGQTECKYGFS
                     LTRLTFDAGGITSQSKTRVDTGLSNPGNSRWFSAATDGNGTFIGDYNGVAAGSDGTTW
                     SLWTDQRAEVENPPSPTRKFGQHAVGSKTPAP"
BASE COUNT         1915 a         3421 c         3354 g         2441 t
ORIGIN      
        1 cgtgatacag gaatggacca gatcgtcgag gctcattgct tgggccgtat cgtacaatac
       61 ggcgactcgg ctgacctccg cgaggcactc gccgagttcg cttcctggag tcccgaagcg
      121 tgggaggagt tttcgcgcca cgcgagaggg gtttactccg ccgtgttttc gtggtcggtg
      181 atgaaatctc gcctccaaac gctgtattcg acacttccgg cgcgcgttgg gggcagaggg
      241 cggctcgcca cggcatgagg atctacaagc cggcattctt ctggtttgtc gtggcgcttg
      301 ccgtcagagc ggcggcggca ctcgccctcc atttgtactc gatctcggcc ggtttcgaag
      361 gtttctaccc cctagcctct ggacatgacg accggcagta ttggaagctt gcggcaagcc
      421 ttgcggacgg aatgaagccg gtggacctcc cgcatgcgta ccccttagtg cttgccttcc
      481 tgtttcgatg tttcggtcct agcctggtgc ttggaaaggc gctgaacatc ctcgcaggct
      541 ctgtgacggt cgctcttggg gtactgatca cgcgggacct gtcacgccac gttacaacat
      601 cgtggcgaaa gactcttcat ccggtcaatg tagctgggtt gttattaacg ttctatcctt
      661 ccgccgtgtt ttactctacg cagctcgtaa aggacccgat tctcgtatta cttggcatgt
      721 ggtctgttta cattgtagtc cggattgtgc atgagcgccg gtttagtctc tgggccttgt
      781 gggttgggag cctcgcggct ctttacctgt ttcgccctta tgctgcctgc gtgatggggt
      841 tgagcgtgtt ggtgttttta acatccttct ggcgcgcaag gctccgcacg cgcgtgacgg
      901 tgttactcat cgccgtgctc accagcgcga gcgtaccaaa tctcttgggc tggggctggt
      961 tcggctccag ttaccttgca ccacttctag accccgagtt tgtgattcag ttccgcgagc
     1021 gtgcatattc gagcggcgga agtgcggttg gcatagagct gaattaccta gagccgctca
     1081 gtttcgtcag ttcgtacagt tatggtctct tgacggtgtt gtttgggcca tttccctggc
     1141 agattcggtc ggcggtgatg tacatcgcac tgcccgagtc tctggtgatg ttgtcgctgg
     1201 gcattgtggc gctgtccaag aagagtaaac cagacgtata tcagcgtgag caacttctgc
     1261 tactaattct agctgtgcta atgtccgctg cgattgccct gttcagcgac aacatagggg
     1321 ctaacacgag gctgcgtctc cttccttgga gcgcactact gatctacgga gcggcaaaat
     1381 gggacagtcg tcgaagaacc tccgtgtact ctacttgatc acgaagtccg aagccggggg
     1441 agcacagtct cacgtgcttt ccctgttgag ggacctaaga agcgagttcg atttcgcact
     1501 cggtgtggga gaagagggcc agttgagcga acgggccagg gaactgggca tctcggtgtt
     1561 tcgcctacgg tacttggtgc atcggccgga cccgcttagg gacccgctgg cggtcggcga
     1621 agtccttggg ttgatccgga ggtggcagcc tacgctgctg cacgcacaca ccgcaaaggc
     1681 ggggctggtc ggccgggcag cggcgcgact cgccgggatc cgatctgtat acacgccgca
     1741 tggttgggcc ttttcggagc gtgctggacg gcttcagcgg gtggcggcga tccgttgcga
     1801 gcggtggttg tccaactggg atgccgagat catcacggtc tccggcgccg aacgtgctgt
     1861 agccgagcgg gcaggactgt ctatgaacgg ccattgtcac gtgattcata acggggttcc
     1921 agacactccg caccgggcgg gacctcggag ttatgagagc ggcgtgatga ttcacgtggg
     1981 gcgattcgtc gccgaaaagg accacgacct cctccttcgt agcctctctc agttgcgctg
     2041 tcgctaccgc cttgttctgg tcggagacgg gccacttcgc tcacgggtgg aggcgcaggc
     2101 cgtggccctg ggtctaagtc gaaacattga cttccttgga acgcgcgacg acatcgctga
     2161 gcttctttct caggctagtt tgttcgtcct tccctcaaag tgggaggcgt tcccaataag
     2221 cgtgcttgaa gcaatgcgcg gaggccttgc ggtagtcgcc actaacgtag gtggtctggc
     2281 ggaggccgtc gacgacggag aaacaggctt ccttgtcccg cgcggtgacg cggtgtcctt
     2341 ggcccagaga ctgagccggc ttctggaatc ccccgcttca gctgccacga tgggagttca
     2401 aggacgggcg cgatatgagc gtctcttcac tgctgatctt atgctgaatc ggactctgga
     2461 gatttatcgg agagccctca tgggctgaga ttggtgcgag agacctatgg gagtgtttgt
     2521 ccaggatgtc tcaagccaac tcttgacccg tgtccgagct cacggtcaac cagtcgctcg
     2581 cccggcgtta atggtgagta gcctggttct caccgacttg actgcacttt gcgtcgccgg
     2641 tgggctgtcc gtgttggggc gatacctctt cggtggtcag ttcacttttt ggctctactt
     2701 tgacatgtgg cccctgctgc tttcttttcc agtgtccttc gctgcaattg atctgtacga
     2761 cgaagtggga atcagcccgc ccgaagaatt gcggcgcacg acgatggcga tgagtctcgt
     2821 gtacctggtc atcggggcgg ggacgtttct gcttcgcgga gcggaagatt actcgcgcgc
     2881 cgtcttcctg gttgcgtgga tgctgtccct cgtgttcgtg ccaatcgcac gccatacggt
     2941 gcggaataac ctctgtaggc gaccatggtg gggacgtccc gcagtcgcca ttgtgaaagg
     3001 cagaagagga gagctcgttc tctcctcgct gctcagccaa gcgtcgcttg ggctaaagat
     3061 tatcggcgtt gtcactgacg acaagagcac cgctcattca atctgtggaa tccccgtcat
     3121 tggcgatctg acttcacttc cgtcagtttc ttcttcaact ggcgttcggc atggaatcat
     3181 tgcgatgccg gcagcgagcc gccgcagact ggctgacgtt gtcgaagcct gccgtcagct
     3241 tctagccaac gttattgtga ttcccgaatg gttcgatccc tcacggccgt gggtggcatc
     3301 gagagacatc aatggtgttc ctgggcttgc tctgcgacag gaattgcttc tgccaggccc
     3361 aaggttgatt aagagatgcc tcgacatcat cggaacgata gtcctctcct gtgtgttcac
     3421 tcctctactg gttgtgaccg ccctatctgt cgtcatctgc tcgccaggta aggtcttcta
     3481 cggacacaga agagttggac agggtggccg gcgcttcaat gcgtggaagt tccgtacgat
     3541 ggtgcgggac ggggatcgga tgctgcggcg gtgtcttgct gaaagcccgg cgctccggaa
     3601 ggaatgggag caggctcaca agctgcgaaa tgacccgcgc gtcattcgcg tgggccattt
     3661 tcttcgacga tcgagcttcg acgaactgcc gcagttgtgg aacgttttga gaggcgagat
     3721 gagcctggtg ggacctcgac caatcgtcga cgatgagatc cgccgttacg gcgagcactt
     3781 tgacctgtac aaggcagtca ggccgggcat tacggggttg tggcaggtct ctggcagaaa
     3841 cgacacgact tacgccgagc gcacaaacct tgacaggtac tacgttcgca actggtcggt
     3901 ctggctggac ctgtacatcc tggcacgcac agtccgcgcg atgctcctcg gccggggagc
     3961 gtactgacgc ccctcatcaa ttcccccccc aaatccgcta gactgaaatc ggaggaatgg
     4021 ccgagcggtt gaaggcggcg gtcttgaaaa ccgcagtact cgaaagggta ccgggggttc
     4081 gaatccctct tcctccgcca cctccttagt tctcgccgaa gtcgctacgg tatgcgtcca
     4141 ccagcttcgc ggcccagctc tcgagtggaa tcagcctgcc gacgaagaag cgcagcaccg
     4201 gcggctcctc gacgaacttc agccccttga tcaagttccg gttccggtgc agccacgtga
     4261 cccggtccac cgcgtactcg atctgcgaca tcgtgtacac gcgccggggc accgcgaacc
     4321 ggaccagctc cagatcggat gggacttcgt tgccctcgcg gtcgcggtcc atggagagcg
     4381 tgccgcgctc catcgtgcga attccggagc agacgtagac cgcggccgcc agcgcgccgg
     4441 ccggataccc gagcgagggt atgtcaggaa ggaacttgtg ggcgtcaacg tggcacgcca
     4501 agccgcccgc gggcgtcacc accggcaccc cgttttccga caaccggttc acgaagtatt
     4561 tcacgaactc ggccgagctg ccggctacct cggtttccac catttcgcgg acgcctactg
     4621 ccatcgcctc cacttccttg gtcgacattc cgccgtacgt gaagaacccc tcatagacag
     4681 gcagccacgg acggatgcgg tccatcagat ccttgcggct tgtggaaatg aatccgcccc
     4741 gggcgctgca actctttcgg ccggagaggt agcaaacatc cgccaggttc atgatctcgc
     4801 ggatgatctc gagaatcgac ttgtcctggt aaccctcctc ccgctgcttg atgaaatagg
     4861 cgttttccga gatcaggctg ccgtccacca ccaggaaaat gccgttctgt gcggccacct
     4921 ctttcactgc gcgtaagttg cccagtgaga acggctggcc tccgagcagg ttggtggtcg
     4981 cctccattcg cacgaaggag atcttatcgg cgccgtactg cgcgatcact ttgcgaaact
     5041 tgtcgatgtc gaggttgccc ttgaacggac aggtgctttg cgtgaccagc gcttcgtcgg
     5101 tgaagatctc gagcaccgtt ccgccggcaa tttcgaagtg cgcccgggtg gtcgtgaagt
     5161 ggtagttcat cggtaccacg tcgcccggct tgacgaacac cttggccagc agatgctcgg
     5221 ctgcacgtcc ctggtgggcc ggcagcaaat actcgtgtcc gaagacgtcc tttaccgccg
     5281 ccgccagctt gtagaagctc tccgagccgg cgtaggcgtc atcggccgtc atcatcgcgg
     5341 caagctggtt gtcgctcatc gagttcgtgc cgctgtcggt cagcatgtcc aggaagatgt
     5401 ccttcgttcg taacaggaag gtgttgtagc ctgcctcctc catagcggcg aggcgctgcc
     5461 cggccgggat cagattcgcc ttctggacga ttctcacctt gtgcatctcg atcgggattt
     5521 ctcttccgtc agacagtctg acactcatga aaaagctcct tctaggaaaa tggggggtac
     5581 tacggtaatc tcgcagtata gtcgatttta agacctgatg tgatgaaatc gctgactgag
     5641 tcccctcagg tataattccc gcttgcccgg aggatccgcc tcgatgcccg ccgccccaga
     5701 aaccgcttcg gtgtctctcc ccgagaacgc gtacaagccg ctcgcacctg gcgagacata
     5761 ccgccccgtc gtgccggcgg cggaagcgcc tcccgaactc accggccggt ccatcggttg
     5821 gggtgtgctg ctctgcgtga tcttcaccgt ggcctcggcc tactcgggcc tcaaggttgg
     5881 ccaggtgatg gaggcggcta tcccgatatc tattctggca atcgggctgg ctcgtatgta
     5941 ccctcgccgc tcctccgtgc tcgagaacgt gatcatcacc ggcattggcg gcgtcgccgg
     6001 ctcggtcgtc gcgggcgcga ttttcacgct gcccgctctc tacattctga agctggaccc
     6061 gcaccccgtc cagacgatct tcatctgcct cgcgggcggc tgccttggca ctcttttcct
     6121 cattccgctg cgccgatatt tcgtccgcga catgcacggt cttcttccgt atccggaggc
     6181 caccgccatt acggaggttc ttatcaccgg ggagaaaggc ggctcacagg cgcggctcct
     6241 gctccaggcc accgccatcg cgggcgtcta cgacttcttc gtcacgacat tccaggtctg
     6301 gaaggagttc gtcgatttcc acttccttcc gatcatgcgc actctgagcg accgggcgaa
     6361 aatcgtcttc aacttcgatg cgattggctt catcctcggc ctcggctacg tgatgggtct
     6421 ccgcagttcg atgattctgt gcgcgggcgg cgtcttgtcc aatttcgtcc tcgtcccgct
     6481 catctggatg atcggaagcc atctgccgga cctcgctgtc tacccggcga agaacccgat
     6541 ctcgcagatg gacgccgggc agatctttcg cggctacgtt cgtttcatcg gcgtaggggc
     6601 catcgcgacc gccggtattt tcggtatcgt caagtcgctt cgcgtcgtgg ccggctcctt
     6661 cgccatcgcc gcccgagcgt ttcgtcgcgg tgaggcgggc ggcatggagc gaacggaccg
     6721 cgatatgtcc gtcgtctcga ttctcgccgg cgtggtcgtc agcgcagtcg ctgtggccat
     6781 tttcctcgga agcctgtctt cgtccgtgac tgtcatcgca atcggtctgc ttcttacgtt
     6841 ggccttctcg ttcttcttca cttccgttgc ggccaacgca attgccacca tcgcccgcaa
     6901 cccggtttcc ggaatgacca tgctcaccat catcatctcg agcgtggttc tgctccgctt
     6961 cggactctcc ggtaccaccg ggatgttctt cgtcatggcg atcgccggga tggtctgcac
     7021 cgcgctctcc gtttcgggcc aggcgatcac cgacttcaag gccggctact ggctcggctc
     7081 cactccggcg gcccagcaga aagtgaagtt tctcggtgtg atcgccgcgg cggcagcggc
     7141 gggactgacc atcgtgttgc tcgcccgcgc cttccagttc ggcgaggcgg ctcctgggga
     7201 tccccgaacc gtcctgccgt cccctcaggc tgcgattatg aaagcgctcg tcgaaggttt
     7261 cgtaggcaga cagccggttg cgtcgatcct ctttggcgca ggcgcattcg tcgccctgat
     7321 catggagatg cttggcgtgc ccgcactgat cttcgctctc ggcatgtacc ttccgctgga
     7381 actcaacaca ccggccctgg tcggcggctt tctggcgcac ttcctcgggc gtcgtgcgga
     7441 gaaggcaggt ggcggccgtg gggcgtcgat ccgcgagcgg ggcgtgatca tcgcctcggg
     7501 attgatggcc ggcggcgcgc tcggaggtgt actcggagcg gccttccggc tcatccccgg
     7561 ctactcggaa agctggatca agacgccgtt ctatgatttc gatccggtgt cccagttcgt
     7621 ttcagccgga ctcttcgccg gcctctgttt gtacgtctgg tttggatcgc tgagaagcag
     7681 cgggcgggag gtgaactcat gagtgcgcgt tttgaagagg tcgccgggaa tgccattctc
     7741 ggaattgaca ccctctgggg tggagacgtc atgtgcccat ccggcacggg ccgcttcatc
     7801 gcggattcct ggttctccga cgagccgctc ccgcatgcct acacacaccc tgccgctgcc
     7861 ctggtgcgtg agtccggcgg cgtctccgcg aaaacactcg accgcgaggc catcgaggcg
     7921 tatctcgacg ccgtcgacgt accggccgcg attcgcggca tgggcgaagc cggcaaagcg
     7981 atgggtggtc tccgaggagc ctacgtctcg aatctcgcgc tctgtttcca gacgatgtgg
     8041 gatctcgtac tggaactgct cggcaagggt gagccggtgc cttacgcccg ctgcgtccac
     8101 gcttccatag gtgcggatcc gtcaccctcc gatcccgtat cgaaacggga gcgcgtcgcc
     8161 gagttgcttt cgcgctgcgg ccaccccaca cgcactccgg acgagctgct ggcggccgtc
     8221 gactcctggc gccgcgcgcg aatggtcccg atggcctccg tccgcgcgct cggagcggcc
     8281 ttcatcgcgc aatttgaccg cctggctgcc gaaaacctga tcccgtatct gccgcccgaa
     8341 ctgcatggcg tgccccgtgc caatattcag tttctgccga tcaccggcgc atggttctcg
     8401 ggttcgatga actaccttgg acgcgcccgg cggtccgacg ggactccgga ctacgaggcg
     8461 acctacgaga tcaacgcctc tctcgaaatc tccgtgcccg agttcgagca gttggtcagc
     8521 cacgaggtcg tgcctggcca cgtcacgaca ttcgcgtatc tccagaacct ctacgttcgg
     8581 agcctgatcg gcttcgaggg ctccgtgctg acgatgaaca cgcgctccgc ggcgctattc
     8641 gagggaatcg ccaacaacgc catcctcatg gcacacgggg tgaccgaaat cgaccaggtg
     8701 cccgacgacg acctccagat cggcctgttg cttgcccttc ttcaggacga tgccaagaac
     8761 cagtcttcgt acctgacctg gaaggagggc gcaccagaag ccgaagtcgc ggcggtcctc
     8821 cgaaaggact tcctggtgtc tgttgagcgg gcggacaaac tttccggcgc ctggggccgg
     8881 cacccactcc tcgggaggat gtacctgccc gcgtaccggg ctgggaccga ccggatagcc
     8941 gagctccgcc gcgtccactc cgcgaagaaa ttgctcccag tgctctacgg ttgcgccggt
     9001 ctggtcgatc tcaccacgat cgacagcgcc ctcgcgtagc ccggcctttc gatgcatccg
     9061 gacggggcgg cgctgcatct caactaaata tgagctttct tcgccttttc gccgcccaga
     9121 cgcttctggc agcgtctttg cttggcgcat cgagcatcca cgagttcacg ctgaactcga
     9181 tcgacggcaa agccgcccca ctttctgagt acaaaggcaa ggtcgttctg ctggtgaacg
     9241 tggccagccg atgcggcttc acgcctcagt acgccgggct tgaggccctg taccggaaat
     9301 acaaggaccg gggactggtg attctcggat ttccggccaa caacttcggc agccaggagc
     9361 ccgggaccaa cgaggagatc aagcagtttt gcagccgcaa gtatgacgtc actttcccga
     9421 tgtacgccaa aatttcggtg aagggcgacg atcaggccgc gctgtatcag ttcctgaccg
     9481 acaagagcgc ccatccggac accggcggcg agatccaatg gaacttcacg aagttcctcg
     9541 ccggtcgtga tggcaagatt ctcgcgcggt tcgagccgaa agtaacgccg gagtccccgg
     9601 aactcacttc ggcgatcgaa aaagcgctcg agtaacggga aactgagccc cgccactcgc
     9661 tgcaagggcg taaccctgag ccctagggcg caggtgtttt cgaacctacg gcgtgctgcc
     9721 cgaacttgcg ggtcggtgac ggcgggttct cgacctcagc acgctggtcc gtccagaggc
     9781 tccaagttgt tccgtctgat cccgccgcga cgccgttgta atcgccgatg aacgtgccgt
     9841 ttccatcggt ggccgccgag aaccagcgtg aattccccgg gttcgacagc ccggtatcca
     9901 cgcgcgtctt cgactgtgat gtaatccccc cggcgtcgaa cgtgagccga gtaagcgaga
     9961 aaccgtactt gcattcagtc tggccttccg agtaagaccg gtccatgtac ccgacatcga
    10021 cccgtccgtc cggagcgacg gccacccacg gatagaactg atcgttcgag tcggtatcca
    10081 cagggtagat gatccaggtc gcgccgccgt ccgtggtgcg gcccaggaac acatcgacat
    10141 tcgtcgcttc ctcggagccg tttcggttat cagcccacac gagataggcg gtgtcgccgc
    10201 tcgcatcgct cgggtcggtg gcaatattgc cggccgcgtt cactcggaac tcgcaacccg
    10261 ttaaaacgct gtcgccgctg accccttccg gatagttgat gtcgtagacc gtgtccacgc
    10321 gtgacggggc gccccacgtc tggcctccat cggttgagtt cacgaccatg tactggttct
    10381 cggccggcgt gttgaagttc tcgaatgcga cgtagacctt cccggtagga gcgatggcag
    10441 gaatcgaata ctggttgaga tcacactcgt tcggcgcgcc ggagagggtc gcgatacaaa
    10501 acgggctgta tccgctgatt tcggtcgccc cactccaccg attgccgtcg tcggacaacg
    10561 acacctgaat cggtgaacgg aagaacggcg ccacgccgcg cgggttttcc tggaaactgg
    10621 tccaggtgac gtatacacgg ttcttgtagg ggctgccggc gtatgcatcg gcggcgatga
    10681 aatccttgtc ctggaacacc tgccgcgcga tacgtccctt ggtgaccgct cccaagccgg
    10741 tcgcgacggc caccgggttg gaccacgtaa atcccccgtc gtccgaatgg gccacgacga
    10801 cgctcggcga ggtgtaactc ccgccgggac cctcgtggtc ggcgatgttg ccgaagtaca
    10861 accggccgtc ggcgttgaac gacaacgccg gatcgccggt gaactgatac ggcgtgatgg
    10921 gtggaacgac ttctcgccag ttctcgccac cgtcggtact caccgagacc gtcccgatca
    10981 ccggcgcgat cgctgtcgcg ccgcggaact taacctggta cgagtggtag gcgccaacca
    11041 cgtgcttggg atcattcggg ttgacggcga tgctcgtttc gttgtgcggc acctcgccgt
    11101 cacaatcgat gagcatgtta ccggtgtaga t
//
LOCUS       DATIFQ010000327         2744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1257785, whole genome shotgun sequence.
ACCESSION   DATIFQ010000327 DATIFQ010000000
VERSION     DATIFQ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257785"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..656
                     /locus_tag="VLE22_15210"
     CDS             270..656
                     /locus_tag="VLE22_15210"
                     /inference="COORDINATES: protein motif:HMM:NF023621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HSB15805.1"
                     /translation="MGFFNTILEKLGVGHKPVVTAPVAPVAPGPVATAPPPPKPVAVV
                     DVVAQLEQRAAANKQKLNWRTSIVDLLKLLEIDSSLTARKELATELGCPAEQMGDSAK
                     MNTWLHKTVLARIAANGGNVPKELLD"
     gene            692..1219
                     /locus_tag="VLE22_15215"
     CDS             692..1219
                     /locus_tag="VLE22_15215"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HSB15806.1"
                     /translation="MQINDILAQMGGFQSIARELGISEAQASTGASALLPAILGGFKK
                     QAQVQPTGLDGLGSLLGGAGGGSLLDAVLAPQPTNVSAGNDLLGQIFGSKDVSRAVAQ
                     GAAAQSGLTPDLLKKMLPLLTMMVAGYMAKQHAGTATQSAPGGTGLGGLLGGLMGGDG
                     NPLDDILGMVGKLNR"
     gene            1275..1529
                     /locus_tag="VLE22_15220"
     CDS             1275..1529
                     /locus_tag="VLE22_15220"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HSB15807.1"
                     /translation="MDWIISLIIGGIVGWLASIVMKTNAQMGWIANVLVGVVGSVLGT
                     WIAGLLGFAATGGISRFLLAFAGAALLIFILSRVGVFKRA"
     gene            complement(1646..2008)
                     /gene="ssrA"
                     /locus_tag="VLE22_15225"
     tmRNA           complement(1646..2008)
                     /gene="ssrA"
                     /locus_tag="VLE22_15225"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2298..>2744
                     /locus_tag="VLE22_15230"
     CDS             2298..>2744
                     /locus_tag="VLE22_15230"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB15808.1"
                     /translation="MSRSRLLVRSHWMAGLLALAVLTPELGRPAVRKTGIIEGYIRDQ
                     AGTGISGAQVRIENSPLGAVADSTGFYRLAKVPVGVVDLSAQFVGYIPVKVTGLKVLS
                     KRITVQDFVLEQQAVDIGEISVMTATNALAPRDAVTTRQGTRPGEAA"
BASE COUNT          480 a          885 c          886 g          493 t
ORIGIN      
        1 ctgctcgagt gggagaagac tcgggtgacg atccccatcg cgccggggaa gtagcgccac
       61 cagtcatatc gcgaccaagt atgaaggcga ggctccggtg atcaaccgga gcctcgcctt
      121 tcgctttccg agtctgaagc tttgttcggc agaaccggcc ctgtatccat acataccctg
      181 cccaattttg ccttgttcgg cagggagatg acgcgttccg gcagcttcgc ggagtcgcgc
      241 cgttccgtcc ggatcacacg gagggcatca tgggattctt caacacaatt ctcgagaagc
      301 tcggtgtcgg ccacaagcca gtcgtcaccg ccccggtcgc gcccgtggct cctggcccgg
      361 ttgccaccgc accaccgccg cccaagccgg tcgccgtcgt cgatgtggtc gcgcagctgg
      421 agcagcgcgc cgcggcgaat aagcagaagc tgaactggcg gacctccatc gtagacctcc
      481 tcaaactact ggagatcgac agcagtctga cggcgcgcaa ggagttggca acggaactcg
      541 gctgcccggc ggagcagatg ggtgactcgg caaagatgaa tacctggctt cacaagaccg
      601 tactcgcccg catcgccgcc aacggtggca acgtgcccaa ggagttgttg gactgaccgg
      661 ctgctgacga tcgatacgag gaaccaggca tatgcagatc aacgatatcc tcgcgcagat
      721 gggcggtttc cagtcaatcg cgcgagagct gggaatcagt gaggcccagg cgtccaccgg
      781 agcctccgcg ctccttccgg cgatcctggg cggattcaag aagcaggcgc aggtccagcc
      841 cacgggcctc gatgggctcg gcagtctgct gggcggggcg ggcggcggct cgctcctgga
      901 cgcggtgttg gcgccccagc cgaccaacgt gagcgccggc aatgacctgc tgggacagat
      961 cttcggctcc aaagacgtga gtcgagccgt cgcgcagggg gccgcggcac agtccggcct
     1021 gaccccggac ttactcaaga agatgctgcc cctcctgacg atgatggtgg ccgggtatat
     1081 ggcgaagcag catgccggca cagcaaccca gtccgcaccc gggggcaccg gcctcggtgg
     1141 cttgctgggc ggcctcatgg gaggcgatgg caacccgctg gatgacatcc tgggcatggt
     1201 tggcaagctg aaccggtaac caaggcagcc cgcccgcagg gcgggccgcc aggccttggc
     1261 atgaggagac tgatatggac tggatcattt ccctgatcat tggcggaatc gtcggctggc
     1321 tggcgagcat tgtgatgaag accaacgccc agatgggttg gatcgccaac gtgctggtgg
     1381 gtgtggtggg atcggtcctc ggcacctgga ttgccggact gcttggcttc gccgccactg
     1441 ggggcatctc ccgctttctg ctggcgtttg ccggcgcggc gctcctgatc ttcattctga
     1501 gcagggtcgg cgtcttcaag cgggcctgag gtcttcaggc gccgcaggtg ctgcaatcgc
     1561 gccgcgggta gcgacgcttt agcccgagca aaaacacaac ggcgccccgt ttgggacgcc
     1621 gttgggactg ccttggactg ctcgaaggtg gaggcggagg gaatcgaacc ctcgtccgag
     1681 taagcttcca gcaccacttc tacgcgcgta ggtactgctc tttgcgtcga tccgggccgg
     1741 tgcagcaccg accttcccgg actctatccc ctaaggtctc gcttctgccg gcggagctac
     1801 ggcagtcgcc agtccggatt tgcgattcga tggcgacgcc ccggacgggc tccgttcatc
     1861 gaaggtgcat gaagcgagct tagctcagct tacgcagcca ttgccagttc agagttggca
     1921 gttagtgctt tccagacggt tttaccaggt gcctggccct gggcgcgctg cgatcccttc
     1981 atcaaacccg tcgaagcctg tcgcccccgg gaatgcaccg gaaagatagc cgctagagca
     2041 ggtccgttca accaaggaac accgaggccc ctaccgccct gccgccttgc cgccttgcct
     2101 ccttgcctcc ttgcctcctt gcctccttgc aacatgtgtc acaaatcacg ctctgccgcg
     2161 tcgccctaac gacaaatctc cggtctcggc cgaggcccct catcccacaa cctgcaggat
     2221 tccgggctgt cgcgcgcacc gtccgggtcc cgcgtaccct gctgcggccc tcccccgcgg
     2281 ccggggagga ggcagtcatg tcgagatcac gtctgctggt acgaagtcac tggatggcgg
     2341 gcctgctggc gcttgcggtg ctcacgccgg agttgggtcg cccggctgtc cgcaagaccg
     2401 gaatcatcga gggctacatc cgggatcagg cgggaacagg catctcgggc gcacaggtcc
     2461 ggatcgagaa ctcccccctc ggcgccgtag ccgattccac cgggttctac cgcctggcga
     2521 aggtgccggt gggtgtggtg gacctcagtg cccagttcgt tggatacatc ccggtcaagg
     2581 tcaccgggct caaggtgctt tcgaagcgga tcacggtgca ggacttcgtg ctggaacaac
     2641 aggcggtgga catcggggag atcagcgtga tgacggcgac gaatgcgctg gcgccccgcg
     2701 acgccgtcac gacccgccag ggaacccgtc ccggtgaggc cgcc
//
LOCUS       DATIFQ010000328         9446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2025345, whole genome shotgun sequence.
ACCESSION   DATIFQ010000328 DATIFQ010000000
VERSION     DATIFQ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025345"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="VLE22_15235"
     CDS             <1..1541
                     /locus_tag="VLE22_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSB15809.1"
                     /translation="ATVFFFARLWRRSGVLTDLEFYELRYSGKAASAVRGFRAVYLGL
                     LFNCMIMATVNLAACKIAAILFGLERWQTLLAVGLLNVVFAAHSGLWGVLVIDMIQFF
                     IKMSAVIAAAYFAVMLPQVGGLSGLVDKLSHVRGPGGVNYLNMMPDFTSNWELAVAIF
                     IMPIAIQWWAVWYPGAEPGGGSYIAQRMLASKSEKDAVGATLFFNIAHYVLRPWPWIL
                     TGLASIIVYPQLSDIQKAFPNLDPTLIGHDIAFPAMLRFMPIGWMGLMLGGLIAANSS
                     TILTHLNWGASYLVHDFYRRFFKPGASEKHYVMAGRFATLLLFLSSSALVFVMETAQD
                     SFNLILQVGAGTGLLYLVRWFWWRVNAWSEVVAMISSFAISVFFVVLGRTGIKFGTHK
                     QLFMTVAFTTVCWVATAFLGPETDHAKLIDFYKKVRPFGPGWRKIRIAAGVSDAEAAE
                     YAKHENIPLSLLGWTTGSVAIWSSLFTVGNFLYGRTGTALILLGVFVVSGAVLVRVIN
                     RLWK"
     gene            1710..4109
                     /locus_tag="VLE22_15240"
     CDS             1710..4109
                     /locus_tag="VLE22_15240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB15810.1"
                     /translation="METLLRDLKYGIRMLLRSPGVTAVAVVALALGIGVNTAIFSVVY
                     AVLLRPLPVRDVDGLVTVAMVSRKINVSDAQPEFTAYARLKESGRWFESIAAASPGTA
                     ALSIGGTDEAVKLWRVTQSFLPALGVGPAFGRNFLPEEDQPGAARVAMLSHSFWQGRL
                     NGDPRALGSTLTVDGQPYSVVGVLPPGFHVDGRPADIYAPVARSLNSREALPVNIYAR
                     LRPGVSVNQAQAEVDAHTKRQIPSPLGWEPRLWSLRDFEVREVRLSLWLLLGAVGLVL
                     LLACANTATLLLARASARRKEIALRTALGAGRPRLVRQVLTESTVLALLGGACGVLVA
                     MVCVEFVPLLAHERLPGLLEQTRVDGVVLAFTLGLSLLTGLVFGAAPALAAPRMDVYD
                     TLKEGGRSGAGDGRRRGWNILIVTETALALVLAIGASLLIRTFFYLRDVAPGFRVDSL
                     LTARITPPPKKFTSREQALAYWEEVIARVREIPGVKAATFAQSLPLTGDHNVMSWPVE
                     GHRITRPEDGPAQWSRVVEREYFRTMQIPLLRGRLFSERDDMSSPKVVIVNEAFARHY
                     WPGQNPIGKHIGGGEVPVFEVIGVVGDVRLEESTKAAPLEVLFHFLQGPTGRIALALR
                     ADPSVYRSPLLMEPAVRRAVMSVDSTQPVTQFAEMRQLISDRIAPKRLSAQLIGAFAT
                     LALVLAAVGIYGVLSFSVAQRTHEIGVRVALGARRGQVLGMVVGEAALLAAVGIGFGI
                     AVALGLTQVMKNLLFGVSETEPWIFAGSAAALLAIAVVASFVPAHRAARVDPLVALRQ
                     E"
     gene            complement(4141..4863)
                     /locus_tag="VLE22_15245"
     CDS             complement(4141..4863)
                     /locus_tag="VLE22_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15811.1"
                     /translation="MRSCTALLVAGAVLPAALSAQTAKGCSLPAVSPIFNAANFSRPT
                     VINNRHFPLVPGTTFVYESTRGKREHVEVVVTHDIKSILRVDCVVVRDTVSVGGEVVE
                     DTFDFFAQDKDGNVWYMGEDTREFKKGLQVWTAGSWEAGANGATPGVIMEAHPLVRDA
                     YRQENLPGEAEDMAQVLSLTASASVPYGSWEGNVLVTRECTPLEPKVAEEKYYALDVG
                     LIRTVTVQGGSAESVLVAISQQ"
     gene            5013..5966
                     /locus_tag="VLE22_15250"
     CDS             5013..5966
                     /locus_tag="VLE22_15250"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HSB15812.1"
                     /translation="MTSWFPSVVEVIGDTPMVELSRITPPGRGRILAKLEYLSPGLSK
                     KDRIARQIIEDAEECSELRPGQTVVELTSGNTGTGLAVVCAVKGYQFVAVMSKGNSAE
                     RARMMRALGAEVMLIDQAPGSVPGQVSGADLDRVEQETRRIVKERGAFRAHQFEHTGA
                     FRAHYLHTGPEFWRQSQHSVELFCDFVGSGTTFAGCAAFLKEQNPAIRCFVVEPVGAC
                     AMAGGPVVRPGHRIQGGGYSMPVLPLLDPALIDGFIAVSDEEAIAASRRLASEEGIFA
                     GFSSGANLAAALRLLQQYPGRTAGIVICDSGLKYLSTDLWE"
     gene            6528..9011
                     /locus_tag="VLE22_15255"
     CDS             6528..9011
                     /locus_tag="VLE22_15255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB15813.1"
                     /translation="MPIASTATEVMMLELLKDLRYGARMLRRSPGLVVVAVLSLALSI
                     GANVANFSLANALLFRPLPVEDPNRLVVLTTSYRDNPYNPSSYADYKDLRDHNQVLSG
                     LAAYFVFPMGLKGKDRPEVVMGQLVTWNFFEVLGVRPAIGRSFLPEEEQAPNGPAVAI
                     LSHKSWRTHFGSDPEIVGKKILINSHPFDVIGVAPKGFYGINTVFAPDVWVPVTTVRQ
                     TMPFPISLTGRYNSWLAMVGRLRPGVSLAQAQAGMDVLAKNLAEQLSPDPQSRKGFTL
                     VEADRSRAGLHQPNDQLRKANVMLMCLVGLVLLIACFNVANLLLARAPQRQKEIAVRL
                     SLGASRWRVLRQLLAESLMLSLMAGAVGMLFALWGIDLLMAFLPTVPGFLIEIDARPD
                     GWVLGFALLLSLVSGVLFGLAPALQMLRPQQLAALKDQSQAVTRSRGKARLQSSLVVA
                     QVAVSVILLVSAGLFIRSLRNALRVDPGFDLRDGLVAEIDLGFGQYDESRGRQFYDRL
                     MEQVEALNGVESASLAVDLPLSGMRIQDHVLVDGYDPAPGERMVMRWNGVGAGYFETL
                     GVPIVQGRGFTRQDREDSRRVAVINEAMARRYWAGRNPVGAFVRRGDDRCEVIGIARD
                     GKYDDLDEQPKPHVYFPLRQGEFVKRFNLVVRTSVPPKSLMATVLAELRRMDSNLPPP
                     RIMTMEQFMQNAIESAGGPGYLISVFGLLALALAMIGVYGLMSYSVSRRTHELGIRMA
                     LGARQGEILGLVLRKGLMIAVVGLLVGLAAAIAVARLLRGLLFEVSPLDPLTFAAVTL
                     ILVITVLAACYLPARRAAQVTPAAALRYE"
BASE COUNT         1563 a         2920 c         2967 g         1996 t
ORIGIN      
        1 tggccacggt gtttttcttt gcacggttgt ggcgccgctc gggcgtgctg accgatctcg
       61 agttttatga actgcgctat tcgggcaagg ccgcgagcgc ggtgcgcggg tttcgcgcgg
      121 tgtacctggg gctgctgttc aactgcatga tcatggcgac cgtcaacctg gcggcgtgca
      181 agatcgcggc gatcctgttc gggctggagc ggtggcagac gctgctggcg gtgggtttgc
      241 tgaacgtggt gttcgcggcc cactcggggc tgtggggcgt gctggtcatc gacatgatcc
      301 agttcttcat caagatgtcg gcggtgatcg cggcggcgta cttcgccgtc atgctccctc
      361 aagtcggcgg actcagcgga ttggttgaca agctgtcgca cgtccgcggg ccgggtggag
      421 tcaattacct caacatgatg cccgacttca ccagcaactg ggaactggcg gtggcgatct
      481 tcatcatgcc gatcgccatc cagtggtggg ccgtctggta tccgggcgcg gagcccggcg
      541 gcggcagcta catcgctcag cgcatgttgg cgtcaaaaag cgagaaggac gctgttggag
      601 caacgctctt cttcaacatc gcccactacg tgctgcgccc gtggccctgg attcttaccg
      661 gtctcgcgtc gatcatcgtt tacccgcaac tctcggacat tcagaaggcg ttccccaatc
      721 tcgatccgac gctgatcggc cacgacatcg cctttccggc gatgctcaga ttcatgccga
      781 tcggctggat gggcctgatg ctcggcggct tgatcgcggc caattcatcg acgatcctca
      841 ctcacctgaa ctggggcgct tcctacctcg tccacgactt ctatcgccgt ttcttcaaac
      901 cgggagccag tgagaaacac tacgtcatgg ccgggcggtt cgcgacgctc ttgctgttcc
      961 tctcttcctc cgcgctggta tttgtcatgg agaccgcgca ggacagcttc aacctgatcc
     1021 ttcaagtcgg cgccggaacc gggttgctct atctggtgcg ctggttctgg tggcgcgtga
     1081 acgcttggag cgaggtggtg gcgatgatca gttcattcgc catctctgta tttttcgtgg
     1141 tgctgggcag gaccggcatc aagtttggca cgcataagca gctcttcatg accgtcgcct
     1201 tcacgaccgt ctgctgggtg gccacggcgt ttctcggtcc cgaaaccgat catgccaagc
     1261 tgatcgactt ctacaagaag gtccgtccct tcggtcccgg ctggcgaaaa atccgcatcg
     1321 cggcgggcgt cagcgacgcg gaagcggccg aatacgccaa gcacgagaac atcccgctgt
     1381 cgttgctcgg ctggacgact ggttcagtgg cgatctggtc gtctctgttt accgtcggca
     1441 acttcctgta tggccgcacg gggactgcct taattttgct cggcgtcttc gtcgtcagcg
     1501 gcgccgttct cgttcgcgtc atcaaccggc tctggaagtg acgtccgggc agagagtgtc
     1561 tgacacagtt cacggcgggc ggcggagaca acgcctcccg ccggtttcgt tttcgcgcgg
     1621 ccacgtcccg aaatcggaca cattccagat gaaaccgctt gatttttgag tgacttgctg
     1681 cggccagccg attgctctcg tcgggtttga tggaaaccct tcttcgcgat cttaagtacg
     1741 gtatccggat gctgttgcgc agccccgggg tgactgcggt cgccgtcgtc gctctggcgc
     1801 tgggtattgg tgtgaatacg gccatcttca gcgtcgtcta tgcggtcctg ctgcggccgc
     1861 tcccagtgcg ggacgtggac ggcctggtca cggtggccat ggtcagccgg aaaatcaacg
     1921 tttccgatgc ccaacccgaa ttcaccgcat acgcccggct gaaggagagc gggcggtggt
     1981 tcgagtccat tgcggccgcg tcccccggca cggccgcgct ctcaatcggc gggacggacg
     2041 aagccgtcaa gctctggcga gtcacgcaaa gcttcctgcc ggcgctcggc gtcgggcctg
     2101 cgttcgggcg taacttcttg cccgaggagg accagcccgg agcggcccgt gtcgcgatgc
     2161 tttcccacag tttctggcag ggccggctca acggcgaccc gcgggctctc gggagtacgc
     2221 tgaccgtcga tggccaaccc tacagcgtgg tcggggtgct gcccccgggt ttccacgtgg
     2281 acggccggcc ggccgacatt tacgcgccag ttgcccggag cctgaactcc cgggaagcgt
     2341 tgccggtaaa catttatgct cgcctcaggc cgggtgtgag cgtcaaccag gctcaagctg
     2401 aggttgacgc ccatacaaaa cgccagatcc cgagccccct gggctgggaa ccgcgtctct
     2461 ggtctctccg cgatttcgag gtccgcgagg tgcgcctgag cctgtggctg ctgctgggag
     2521 cggtcgggct ggtgttactg ctcgcttgcg ccaacacagc cacgttgctg ttggctcgcg
     2581 ccagcgcacg gcggaaagag atcgccctgc gaaccgcgct cggcgccggc aggccgcgtc
     2641 tggtgcggca ggtgctcact gagagcacgg ttctcgccct gctcggcggc gcctgcggcg
     2701 tgctcgttgc gatggtatgc gtggagttcg ttcctctgct cgcgcacgaa cggcttcccg
     2761 ggctgctcga acaaacgcgt gtggacggcg tcgtcctggc cttcacactc ggactgtccc
     2821 tgttgactgg tctcgtgttt ggcgccgctc ctgcgctcgc tgcgccgcga atggatgtct
     2881 acgacacgct gaaggagggt ggacggtccg gcgccggtga tgggcgaagg cgtggctgga
     2941 acatcctgat tgtgaccgaa accgccctgg cgctcgtgct cgccatcggc gcgagcctgt
     3001 tgattcggac attcttctat ctccgcgacg tcgcgccggg tttccgtgtc gacagcctcc
     3061 tgaccgcgcg aatcacaccg ccgccgaaga agttcacctc gcgcgagcag gccctcgcct
     3121 actgggagga ggtgatcgcg cgcgttcgcg aaattcccgg cgtgaaggcc gccacgttcg
     3181 ctcagtcgct tccgctcacc ggtgaccata acgtgatgtc ctggccggtc gaagggcacc
     3241 gcatcacccg gccggaggat ggtccggccc aatggtcccg cgtggtggag agggagtact
     3301 tccggacgat gcaaatccct ctgcttcggg ggcggctgtt cagcgagcgt gacgacatga
     3361 gctcgccgaa ggtcgtcatc gtgaacgaag ccttcgcgcg ccactactgg cccggtcaga
     3421 atcccatcgg taagcacatc ggcggcggtg aggttccggt gtttgaagtc attggcgtcg
     3481 tcggggacgt gcgactcgag gagtcgacca aagcagcgcc gctcgaggta cttttccatt
     3541 tcctgcaggg gccgacgggc cgcatcgccc tggcgttgcg ggccgatccc tcggtgtacc
     3601 gaagtccgct gctgatggag ccggccgtcc ggcgagccgt catgtcagtc gacagcactc
     3661 aacccgtaac gcagttcgct gagatgcggc agctcatctc ggaccggatc gcgccgaaga
     3721 ggctttccgc gcagttgatc ggggcgtttg ccacgttggc gctggtgctg gcggcggtcg
     3781 gcatctacgg cgttctctcg ttctctgtcg cccagcggac gcacgaaatc ggcgtgaggg
     3841 tcgcgctcgg cgcccggcga ggacaagtgc ttggcatggt cgtcggagag gctgccctgc
     3901 tcgcggccgt cggtatcggc ttcggcatcg ccgtcgcgct cggtctcacg caggtaatga
     3961 agaacctgct gttcggggtc agcgaaaccg aaccgtggat cttcgccgga agcgccgccg
     4021 ccctcctcgc gatcgcggtc gtggcctcct ttgtcccggc tcatcgtgct gcgcgcgtcg
     4081 acccactcgt ggcccttcgg caggagtgac taagaccact ctcgcaggga ccccgagaat
     4141 tcactgttga ctgatggcca cgagcactga ctccgccgat cccccttgga cggtgaccgt
     4201 cctgatcaag ccaacgtcca acgcatagta cttttcttcg gccactttgg gttctagcgg
     4261 cgtacactcc ctggtgacca ggacgttgcc ctcccacgag ccataaggta cggaggccga
     4321 tgccgtcagg ctcagcactt gagccatgtc ctcagcctct cccggtaggt tctcttggcg
     4381 atacgcatca cgtacaagtg gatgggcttc catgatgaca cccggcgtgg cgccgttcgc
     4441 gcccgcttcc caggagcccg cggtccatac ctgcaaaccc ttcttaaact ctctggtgtc
     4501 ttcccccatg taccagacgt tgccgtcctt gtcctgcgca aagaaatcga atgtgtcctc
     4561 caccacctcg ccgccaaccg acacggtgtc cctgacgacg acacaatcca cccgaaggat
     4621 actcttaatg tcgtgggtta cgacgacctc gacgtgctca cgcttgccac gcgtcgattc
     4681 gtagacgaac gtggtgccgg ggaccagggg gaagtgtcgg ttgttgatca cggttggccg
     4741 ggagaagttc gcggcgttaa agattggtga gaccgccggc agcgagcaac cctttgccgt
     4801 ctgggcagat aacgccgcag gcaacacagc ccctgcgacg agcaaagcgg tgcatgatct
     4861 catgtgcacc ccctttagcc gggatttcca ttctggcatc ccaggtctgc tgccagtgta
     4921 ccccccggcc gcgtttgatg tacgccaagg tcttatgcag ttaaactgga cctggtgcaa
     4981 gagtctccgg acaccggaaa caggttggat tcatgacttc ttggtttcct tcggtggttg
     5041 aggtgattgg tgacacgccg atggtggaac tgtcccgcat caccccaccc ggccggggac
     5101 gcatcctcgc caagttggag tatctctcgc ctggcttgtc gaagaaagac cgcatcgctc
     5161 gccagatcat cgaagacgcg gaagaatgca gcgagttgcg gccaggccag accgtggtgg
     5221 agctcaccag cggcaacacg gggacaggcc tcgccgtcgt ctgtgcggta aaaggttatc
     5281 aattcgtggc cgtgatgtcg aaggggaatt ccgccgagcg cgcccgcatg atgcgggcct
     5341 tgggagcgga ggtcatgttg attgaccagg cgcccggctc cgtgcccggc caggtttccg
     5401 gcgccgacct ggaccgggtt gagcaggaga ctcggcgcat tgtcaaagag agaggcgcct
     5461 ttcgcgctca ccagtttgag cacaccggcg catttcgagc acactacttg cataccggac
     5521 cggagttctg gcgccagtct caacactcgg tagagttgtt ctgcgatttt gtcggaagcg
     5581 gcacgacgtt cgccggctgc gcggccttcc tgaaagaaca gaatccagcc atacgttgtt
     5641 tcgtggtgga acctgtgggc gcctgcgcga tggcgggagg accagtcgtt cggcccggcc
     5701 accgcattca aggcggcggc tactcaatgc cggtcctgcc gcttctcgac ccggccctga
     5761 tcgacggttt catcgcagtc tccgatgagg aggcgattgc cgccagccgc cgtctggcca
     5821 gcgaggaagg catcttcgcc ggcttctctt cgggcgccaa cctcgccgcc gccctgcgat
     5881 tgcttcagca gtacccgggc cggacggccg gaatcgtcat ctgcgattcc ggactgaagt
     5941 acctgagtac ggacctctgg gagtagccgg agctttacgt agaaaaatag tcggggcgct
     6001 tctcaatcac tcggctgcgg ttgcaggtag gccggtcgca gtccatctat gcgggtggtg
     6061 ggcatacctt ggaggcgctc ggcagcatcg ctttgttcat tcgctcccgc caatccgtct
     6121 tcaacgcgga ccgtccagtc ctctttagcc gatccaagga cctcaaacag cattctgcga
     6181 taggcctcgc gacgctcttc tgctgtgggt cctccgttct ttggctgccg ggcgaacacg
     6241 tcttcagtat cctcctgcct gctattctgg ccgtcggacg acacgttgca tagaacctcg
     6301 agtgcaaatc gctccagaac ctcgatgctt ctgggtccag tactgttcat cctctgggat
     6361 tgcgcatttg ttaggggccg agcgatgcgc tccctgaatg gacccggacg cttgtgccac
     6421 ttctgggatt acagggtccc aaaaccggac agcctcaccg ccacgccacc tcgcctcaat
     6481 agttaactct ctgtgggtgt tcgacttaac ctcgatcgtt gggtggaatg ccgattgcga
     6541 gtacggccac ggaggtcatg atgctggaac ttcttaagga ccttcgctac ggagcacgaa
     6601 tgcttcgtag gagcccaggc cttgttgtag tagccgtgtt gtccctggcc ctgagcatcg
     6661 gcgccaatgt cgccaatttc agcctggcca acgcgctcct gttccggccg ttaccggttg
     6721 aggatcccaa ccggctcgtc gtcctaacga catcctaccg ggacaatcca tacaacccct
     6781 cgtcgtacgc cgactataag gaccttcgag accacaacca ggtcttgtcc ggattggccg
     6841 cgtatttcgt cttcccaatg ggcttgaagg gcaaagatcg gccggaagtg gtcatgggac
     6901 agttggtcac ctggaacttc tttgaagttc tcggcgtgcg gcccgcgatc ggccgttcct
     6961 tcctaccgga agaggagcaa gctccgaacg ggcccgcagt cgccatcctt agtcataaat
     7021 cctggcgcac ccacttcggg tccgatccgg agatcgtcgg caagaagata ctcatcaact
     7081 cccacccctt cgatgtcatc ggtgtagcgc cgaaaggctt ctacggtatc aacactgtct
     7141 ttgcgcccga cgtctgggtg cctgtgacca cggtgcgcca gaccatgccc ttcccgatct
     7201 ctcttacggg aaggtataac agttggctcg ccatggtagg tcgcctgaga cccggagtga
     7261 gtctggcaca agctcaggca ggaatggacg tcctcgccaa gaacttggcc gaacagttat
     7321 cgccggatcc gcaaagccgc aagggcttca cgttggtgga ggccgatcgc agccgcgccg
     7381 gtctgcacca gcccaacgat cagttgagga aggccaacgt catgctgatg tgcctcgtgg
     7441 gcctggtgct gctgattgcc tgcttcaacg tggccaatct cctgttggcc agggccccgc
     7501 aacgccagaa ggaaattgcc gtacgcctgt cgctaggggc ctctcggtgg cgcgttctgc
     7561 ggcagctcct cgccgagagc ctgatgttga gcctgatggc cggtgcggtg ggaatgctgt
     7621 tcgccctttg gggcattgac ctgctgatgg cgttccttcc gaccgtcccg ggtttcctga
     7681 tcgaaatcga cgcccgcccg gatggctggg tgctcggctt cgcgcttctg ctgtcgctgg
     7741 tcagcggggt cctcttcgga ttggcgccgg ctctccagat gctaaggccc cagcagcttg
     7801 cggcgctcaa ggatcagtca caagcggtga ccaggagccg gggaaaggct cgtctgcaaa
     7861 gtagcctcgt tgtggctcag gtggccgtgt ccgtcattct gcttgttagc gcgggtcttt
     7921 tcatccggag ccttcgaaac gcactccgcg tcgatccggg ctttgacctc agggatggcc
     7981 ttgtggcgga aattgatctg ggtttcgggc agtacgacga gtccaggggc agacaattct
     8041 acgaccggct catggagcag gtcgaggccc tcaacggggt tgagtcagcc tcgctggccg
     8101 tcgacctgcc gctctcggga atgcggattc aagaccacgt tctcgtggat ggttacgacc
     8161 ccgcccccgg cgagcggatg gtgatgcggt ggaatggtgt tggcgccggc tacttcgaaa
     8221 cgctgggcgt tccgatcgtc cagggtcggg gatttaccag gcaggaccgg gaggacagcc
     8281 ggcgtgtcgc cgtaatcaat gaggcgatgg cccggagata ttgggccggg cgaaacccag
     8341 tcggcgcctt cgtccggcgg ggtgacgaca gatgcgaggt tatcggaatc gcaagggacg
     8401 gcaagtacga cgatctcgac gagcaaccca agccgcatgt ctatttcccc ttgcgtcagg
     8461 gcgaattcgt aaagcggttc aatttggtgg tccggacgtc tgtgccgccg aaatccctga
     8521 tggcgacggt gctcgccgag ctccgacgca tggattcgaa cttgccgccg cctcggatca
     8581 tgaccatgga gcagttcatg caaaacgcca tcgagtccgc gggagggccg ggctacctga
     8641 tttcggtttt cggactgctg gccctggcgc tggccatgat aggcgtttac ggactcatgt
     8701 cttacagcgt cagtcggcgg acgcacgagt tgggcattcg catggcgctc ggcgcgcggc
     8761 agggagagat tctgggtctc gtcctgagaa agggcctgat gatcgcagtg gtcggcctgt
     8821 tggtcgggct ggccgctgcg atcgcggtag cgcgcctgct gcggggactc ctcttcgaag
     8881 tcagtcccct tgatccgctg acgttcgctg cggtgacgct catcctcgtc atcaccgtcc
     8941 tggccgcctg ctacctcccg gcgcgccggg cggcgcaggt cactcccgca gcggcgctgc
     9001 gatacgagtg acgagcgccg cctcgctccg atcgcagtcc ggtttcatcc cggaactgtg
     9061 gtcagctttg tcggaatccc cgtttctgcg gcgttggtag cgcaaaaagg tgcgcctgcc
     9121 acgtacactg ttcattttct ccgggcggtc ggttgaagcc cgtcgaacca gcttccgtgc
     9181 gtgcccgagc catgttttca gtgttttctc tcaagccatc cgccgctttc agtgacgccg
     9241 agcgggcccg tgagccgtcg ttcggattgg accttgaatt gctccgtgta gggtgcggag
     9301 tgaggaaatg gttctgacgc tcgccagcga cgaagtccgg gaagtccata acgcgctgtt
     9361 ggacgcacat tcggaggtcc ttcgtaagtt gagcctggaa ggcttcccga gccgcgagct
     9421 cggactggaa ctgtgccgga ggaagt
//
LOCUS       DATIFQ010000329         3457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2026148, whole genome shotgun sequence.
ACCESSION   DATIFQ010000329 DATIFQ010000000
VERSION     DATIFQ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026148"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..2279)
                     /locus_tag="VLE22_15260"
     CDS             complement(153..2279)
                     /locus_tag="VLE22_15260"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HSB15814.1"
                     /translation="MKQTVLLFATAAGMLFAQGPQWKEAIRDDHPRLFFNRDTFGAVK
                     ARAMGEEAALFAAMKGRADELAAKALEHGDYGTQAAEAAFVFLVTGETRYRDLGKKLL
                     ETSVAHYRDCHEQNKSVNWYSFSRINGWAAYDWLFNEMTPEERRRLGLAFLDATGFEQ
                     PTRARRAFDRENWGGTESGFYSTPSQLWYAGLATFGEGIDDERAERYLGEGHRLFINL
                     LEHRRGAASDDGGSASGALNYALAAYPWAEFNFFHTYRSATGEDIAIEWPYVAYLPSY
                     IFWNWLPGAHEFGYGDAYHTANMIGVSGLNIHLSQIVQFYAATKPECAAFARWLLDQT
                     PREKGSPFPFARFLLTSMPDVAPAKDPERRMPMARHFENMGQTFFRSGSGPDDTYAMF
                     VSGGVLEMHKQFDHNSFTIYRKGFLALDTGTRPEPGEHLTHYYSRTVAHNTILIRMPG
                     EQMPRYWGNPAPEEQPAPVPNDGGQREVLGSKVVVFETGPEYSYAAGDATPVYHEKKC
                     RMALRQFVFLPPDHFVVFDRVSATQPEFKKTWLLHTAAEPQIRGSEFSADQEEGRLFC
                     RTLLPEKAEIGKVGGPGKQFWSDGQNWPLPKGYRTPDTTPLLGQWRVEVSPGRPAADD
                     VFLHLIQAGDRSVERMADSKLVRRGDRVGVRFRYRNAEWEVLFSTQGPAAGHVRLRRG
                     GRAVVDRELAREVMRQAGLWGEGEAR"
     gene            complement(2284..>3457)
                     /locus_tag="VLE22_15265"
     CDS             complement(2284..>3457)
                     /locus_tag="VLE22_15265"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSB15815.1"
                     /translation="GAMWGVARVIRGGSFVSTISRWARGYRSSMDPDYRSRFTGFRVC
                     RSLPRSAPARTAEADAKWLDTFNRPPAGFENATGPLSPLTEGIASATEWKARKEALRA
                     KWRKLLGAPDIAPPAPRARLVRTFEEQNYTGKLMYLQVEPDHWEKIFLMAPARRIRRP
                     LPVVIVPYYDVDTPAGTNMGGRSFMPPSVRSFAYMAAQQGFLAVAIRWFGESYAESYP
                     EAVATLKMKHPNCTGLGKWVWDSQRLLDYLYTLPEVDRSRIGMIGHSLGGKMTLYATA
                     MDDRITAAVSSELGIGLSFSNYDDYWYFGGFIGEWDKATDHHELLAMTAPRPFLLIGG
                     DDSDNDKSWYYINAARKVYGLFGEPTHIGYFNHRKGHTPTPEAVQRSMEWLARFLE"
BASE COUNT          587 a         1283 c          938 g          649 t
ORIGIN      
        1 cgaactcttc ttttcctgga ccaagcggaa cctgcgcatc gagaaaccgc cgtgaagact
       61 caggtctgga ccagtaccta ggtcctgacc gcgcttgtga agactccatt tcggcattgt
      121 tgcgaaatgg cttcctaccc caggtcttct ccttacctgg cctctccctc cccccacagc
      181 cccgcctgcc gcatcacctc acgcgccagt tcacgatcca ccaccgccct gccgccacgc
      241 cgcaaccgca cgtgccctgc cgccggaccc tgtgtgctga acagcacctc ccactctgca
      301 tttcgataac ggaaacgcac accgacgcga tcccctctcc gcaccagctt cgaatccgcc
      361 attctctcca cggacctgtc gcccgcctgt atcagatgca ggaagacgtc gtccgccgcc
      421 ggccggcctg gcgaaacctc aactctccac tggccgagca gtggcgtcgt gtccggcgtt
      481 cgatagccct tcggaagcgg ccagttctgg ccgtccgacc agaactgctt ccccggcccg
      541 ccgaccttcc cgatctccgc cttctccggc agcagcgtcc ggcagaacag ccgcccttcc
      601 tcctgatcgg ccgagaactc actgccccgg atctgcggct ccgccgccgt gtgcagcagc
      661 catgtcttct taaactccgg ctgcgtcgcg ctcacccggt cgaacaccac gaagtggtcc
      721 ggcggcagga acacgaattg ccgtagcgcc atccggcatt ttttctcgtg atacaccggc
      781 gtggcgtctc ccgcggcgta cgaatactcc ggccccgtct cgaacacaac caccttcgac
      841 ccgagcactt cccgctggcc gccgtcgttc ggtacgggcg ccggttgctc ctccggcgcg
      901 ggattccccc aatagcgcgg catctgctcc cccggcatcc ggatcaggat cgtgttgtgc
      961 gccaccgtgc gcgagtagta atgcgtcagg tgctcccccg gttccggccg tgtgcccgtg
     1021 tcgagcgcca ggaagccttt cctgtaaatc gtgaagctgt tgtggtcgaa ctgcttgtgc
     1081 atctcgagca cgccgcccga aacaaacatc gcgtacgtgt cgtccggtcc cgaacccgac
     1141 cggaaaaatg tctgccccat gttctcgaag tgccgggcca ttggcatccg ccgctccgga
     1201 tctttcgccg gcgcaacgtc cggcatcgac gtcagcagga accgcgcgaa cgggaacggg
     1261 ctccccttct cgcgcggagt ttggtcgagc agccaccgcg cgaacgccgc gcactccggc
     1321 ttggtcgccg catagaactg gacaatctgt gagagatgaa tgttcagccc gctgacaccg
     1381 atcatgttcg ccgtgtgata cgcgtcgccg tagccgaatt cgtgcgctcc cggcagccag
     1441 ttccagaaga tgtacgacgg caggtacgcg acatacggcc actcgatcgc gatgtcctcc
     1501 cccgtcgccg accggtacgt gtggaagaag ttgaactcgg cccacgggta cgccgccagc
     1561 gcgtagttga gagcgccgga cgccgacccg ccatcatccg acgccgcgcc ccgcctgtgc
     1621 tccagcagat tgatgaacag ccggtgcccc tcgccgagat agcgctcggc ccgctcatcg
     1681 tcgatgccct cgccgaacgt ggccagcccc gcgtaccata actggctcgg tgtgctgtaa
     1741 aaccccgact ccgttcctcc ccagttctcg cggtcgaaag cccttcgagc ccgggtcggc
     1801 tgctcgaagc ctgtggcgtc gagaaacgcc aggccgagcc gccggcgctc ctccggcgtc
     1861 atctcgttga acagccagtc atacgcagcc cacccgttga tccgcgagaa cgaataccag
     1921 ttcacactct tgttctgctc atggcagtcg cggtagtgcg cgacactggt ttccagcagc
     1981 ttctttccca ggtccctgta ccttgtctca cccgtcacca gaaacacgaa cgcagcctcc
     2041 gccgcctgcg tgccgtaatc gccgtgctcc agcgccttgg ctgcaagctc atcggcgcgg
     2101 cctttcatgg cggcgaacag agctgcttct tctcccatcg cacgcgcctt gacggctccg
     2161 aacgtatcgc ggttgaaaaa caaacgcgga tggtcgtccc ggatcgcctc cttccactgc
     2221 ggaccctgcg caaacaacat tcccgcggca gtcgcgaaca gcaacaccgt ttgcttcatc
     2281 tccctactcc aggaacctcg ccagccactc catcgaccgt tgcaccgcct ccggcgtcgg
     2341 cgtgtgtccc ttccggtgat tgaagtaccc gatgtgcgtc ggttcgccga acagcccgta
     2401 caccttccgc gccgcgttga tgtagtacca gctcttgtcg ttgtccgaat cgtccccgcc
     2461 gatcagcaga aacggccgcg gcgcggtcat cgccagcagt tcgtgatggt ccgtcgcctt
     2521 gtcccactcc ccgatgaatc cgccgaaata ccagtaatcg tcgtagttcg aaaatgacag
     2581 cccgatgcca agctccgacg acaccgccgc cgtgatccgg tcgtccatcg ccgtcgcgta
     2641 gagagtcatt ttgccgccga gcgagtgccc gatcatcccg atccggcttc ggtccacctc
     2701 tgggagcgta tacagatagt cgagcagccg ctgtgagtcc cacacccact tgccgagccc
     2761 agtgcagtta ggatgcttca tcttcagcgt cgccaccgcc tccgggtacg attcggcgta
     2821 actctccccg aaccagcgga tggcaaccgc caggaacccc tgctgcgccg ccatgtacgc
     2881 gaagctccgc acgcttggcg gcataaacga tcgtccgccc atgttcgtcc ccgccggcgt
     2941 atcgacgtcg tagtacggca cgatcaccac cggcagcggc cgcctgatcc gcctcgccgg
     3001 cgccatgagg aaaatcttct cccagtgatc gggttccacc tgcaggtaca tcagcttgcc
     3061 cgtgtaattc tgctcctcga acgtgcggac cagccgtgcc cgtggcgctg gcggcgcgat
     3121 gtccggcgct cccagcagct ttcgccactt cgcccgcagc gcttctttcc gcgctttcca
     3181 ctccgtcgcg ctcgctatgc cttccgtaag cggagagagc ggaccggtcg cgttctcgaa
     3241 gccggccggc ggccggttga acgtgtcgag ccacttcgcg tccgcctccg ctgtgcgagc
     3301 tggagcggag cgtggcaagc tccggcatac acggaatcct gtgaaccggc ttcggtaatc
     3361 cgggtccatg gacgaacggt acccccgtgc ccaccgcgaa atggtgctga cgaacgaccc
     3421 cccgcggatc actcgcgcca cgccccacat cgccccc
//
LOCUS       DATIFQ010000330         2712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1067610, whole genome shotgun sequence.
ACCESSION   DATIFQ010000330 DATIFQ010000000
VERSION     DATIFQ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067610"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="VLE22_15270"
     CDS             complement(<1..1478)
                     /locus_tag="VLE22_15270"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSB15816.1"
                     /translation="MTRRQFVSLPATLAVTGQPSRAQTTYPGTLYRDYSRCLPDFLRN
                     LASAAYRRRNADLARLTSAEAVQRRQQWARDTFWKLVGGMPERTALNARTVGSFERPG
                     YRLEKILYESRPGFHIGGNLYIPTQAQPPFPGVLFQMGHTRNGKAAREYQRCCQALAR
                     LGYLVLAFDPMGQGERVYYPNASLTGTRLPSSDDEHTVPGKQMLLYGDTSSRLQVWDA
                     IRSLDLLAAHPMVDPKRLGSTGQSGGGTLTMLLGCVDERLAAAVVVSGNTENLACADF
                     NPPGSTDDAEQNFLDSGPLGFDRWDLLYPMAPKPLLITVSDKDFFGTYSSQYISNGWE
                     EFKKLKKVYEVLGKPERLEWGGTPLPHSIDYDMRLLVYNWFGRWLKNDAARIEKEPPA
                     QPEADKELWVSDSGNLVKSLHGQTPFSLNKARNVVKEAAPLERLLRIERSSPAHLDVL
                     RRVPGMVCQIAAVEVRSEASVWIPAWLYLPHKQDRQKPVLLVL"
     gene            complement(1482..>2712)
                     /locus_tag="VLE22_15275"
     CDS             complement(1482..>2712)
                     /locus_tag="VLE22_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15817.1"
                     /translation="SHYLLFAPRFGFSYDVTGQGRTILRGGYGIFYQPPFVEAFNNMV
                     DSAPFSPQFYRYGVDFMNPYEGIRNPFPEEFGPKQPAADSAFQLPMAAVSYSGDWRPA
                     QIQSWNVTLEHQLRPDLLVRGAYVASKGTYLGYNSDLNAALYVPGASEDDIDARRPYQ
                     DFTSVIQDEAAANSIYNSFQATVEKRFAKGFSVLANYTWAKAIDPVSYQTDLDGINVI
                     NPFNVRAYRSVSDFDIPHRLVVSYLWQLPSFKDRSPAMRWVLGDWQTSGIWNWQAGFP
                     VNVDSGEDRALSGVGNDNADLVGDPYLDPNRSRGDVINRYFNTDAFEAAKLGTFGNSG
                     RNILRGPGTFNVDFSAMKNILVSERVKLQLRGEFFNFFNTPLLNNPSSSVSSGRFGQI
                     TSARGPRIVQFALKINF"
BASE COUNT          525 a          788 c          883 g          516 t
ORIGIN      
        1 agtacgagca ggactggctt ctgcctatcc tgcttgtgcg gaaggtacag ccacgccggg
       61 atccatacgg aggcttcgct ccgcacttcg acggctgcga tctggcagac catgcccggc
      121 acacggcgaa gtacatcgag atgagcgggg ctcgaccgtt cgattcgaag gagacgttcg
      181 agcggggccg cctcttttac cacgtttcgg gccttgttca gggagaacgg cgtctgtccg
      241 tggagcgact tgacgagatt gcccgagtcc gacacccaga gttccttgtc cgcttccggc
      301 tgggccggag gctctttctc aatgcgtgcc gcgtcgttct tgagccagcg gccgaaccag
      361 ttgtagacga gcagccgcat gtcatagtcg atggagtggg ggaggggtgt gccgccccat
      421 tccagtcgct ccggctttcc gagaacttcg taaaccttct tcagcttctt gaactcttcc
      481 cagccgttgc tgatgtactg cgaggagtac gtgccgaaga agtccttgtc gctcacggtg
      541 atcagaagag gcttcggcgc catagggtag agcagatccc agcggtcaaa acccagtggc
      601 ccggagtcga ggaagttctg ctccgcatcg tcggtcgagc caggcgggtt gaagtccgca
      661 cacgcgagat tctccgtgtt cccactcacg accacggcgg cggcaagccg ctcgtccacg
      721 catccgagca acatggtcag cgttccgccg ccggattgtc ctgtcgaccc aaggcgcttg
      781 gggtcgacca tcgggtgagc ggcaagcagg tcgaggctcc ggatggcgtc ccagacctgg
      841 agccgggaac tggtgtcacc gtatagcagc atctgcttac cgggtaccgt gtgctcatcg
      901 tcggaggagg gcaaccgggt gccggtgagg ctcgcattcg gatagtaaac ccgctcaccc
      961 tgacccatcg gatcgaacgc cagcacaaga tatcccagac gcgccagcgc ctgacagcag
     1021 cgctggtact cgcgggcagc tttgccgttt cgggtgtggc ccatctggaa aagcactccc
     1081 gggaacgggg gctgtgcctg cgtcggaatg tacagattgc cgccaatgtg gaaaccgggc
     1141 cggctctcgt acaggatctt ctccaggcgg tagccgggac gctcgaacga tccgacggtt
     1201 cgggcgttca gagcggtccg ttccggcatg ccgccgacca gtttccagaa tgtgtcgcgg
     1261 gcccactgct gccgccgttg gaccgcttcg gccgaggtca atcgagcgag atcggcgttc
     1321 ctgcggcgat aggcggcgga ggcaaggttg cgaaggaagt cgggtaagca ccgcgagtag
     1381 tcgcggtaca aggtgcccgg gtaggtcgtc tgggcacgag acggctgccc tgtcacggcc
     1441 aaggtggccg gcagggagac gaattgtcgt ctggtcatag gtcagaagtt gatcttcaac
     1501 gcaaactgaa cgatccgcgg accccgggcg ctggtgatct gtccgaagcg tcccgagctc
     1561 accgaagaac tcggattgtt gagcagaggt gtgttgaaga aattgaaaaa ctcgccgcgc
     1621 agttgcagct tcacccgttc gctaaccaga atgttcttca tcgccgagaa gtcgacgttg
     1681 aaggttccag gcccgcgcag aatattgcgt cccgagttgc cgaaggtgcc gagctttgcc
     1741 gcctcgaatg cgtcggtgtt gaagtaacgg ttgatgacat cgcccctgga ccggttcgga
     1801 tcgaggtaag gatccccgac aaggtcggca ttgtcgttcc cgacgccgct cagtgcccgg
     1861 tcctcgccgg aatccacgtt caccgggaag ccggcctgcc agttccagat tccgctggtc
     1921 tgccagtcgc ccaggaccca ccgcatggcc ggcgacctat ccttgaagct cgggagctgc
     1981 caaaggtagg aaacgaccag ccggtgcgga atatcgaagt ccgacacgct acgataggcc
     2041 cggacgttaa acggattgat cacgttgata ccgtcgaggt cggtctggta ggagacgggg
     2101 tcgatcgctt tggcccacgt gtagttcgcc agcacggaga agcccttcgc gaagcgcttt
     2161 tcgacagtgg cctggaagga gttgtagatc gagttggcgg cggcttcgtc ctggatgacg
     2221 ctggtgaagt cctgataggg acggcgggca tcgatgtcgt cttcgctggc tccgggcaca
     2281 tagagcgcgg cgttcaagtc cgagttgtag ccgagataag tccccttcga cgcgacatag
     2341 gctccgcgta cgagcaggtc aggccgaagc tggtgctcaa gagtgacgtt ccagctctgg
     2401 atctgtgccg ggcgccagtc accggaatac gacacggcgg ccatcggaag ctggaacgcc
     2461 gagtctgccg ccggctgctt cgggccgaac tcctcgggga acgggttcct gattccctcg
     2521 taaggattca tgaagtccac gccataacgg tagaactgcg gactgaaggg agcgctatcc
     2581 accatgttgt tgaacgcctc gacgaacggg ggctggtaga agatgccgta accgccacgg
     2641 aggatggtgc ggccctggcc agtcacatca tacgagaaac cgaagcgcgg ggcgaatagc
     2701 aagtagtggg aa
//
LOCUS       DATIFQ010000331         4583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1260016, whole genome shotgun sequence.
ACCESSION   DATIFQ010000331 DATIFQ010000000
VERSION     DATIFQ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260016"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VLE22_15280"
     CDS             complement(<1..645)
                     /locus_tag="VLE22_15280"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HSB15818.1"
                     /translation="MTTRRTFLSGLLAATLGAADSKTMTSRRAKADSPATADPTASFW
                     KNAPVLTFETGRFGEKYPAFRTEVRSMWTMANLYILFTCPYNDLYMNPNPQTKRDTWG
                     LWDLDVAEVFIGWDLKDIRRYKEFELSPQGEWIDLDVNKNSEPGVFDDKWNAGMESAA
                     RIDRSRRVWFGEMKIPFPSIDPRPPQPGREFRINLYRIQSKKRTFMAWQPTQNES"
     gene            681..1523
                     /locus_tag="VLE22_15285"
     CDS             681..1523
                     /locus_tag="VLE22_15285"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HSB15819.1"
                     /translation="MTSTISRRHLCLGLAGSTGAVQASATIARTASGYTDPRKVLLWE
                     STRKEVRELLDAGQLKAMIIPTASTEQHNEHLAMIHDTASVTLVAQQAALRLYPRVMV
                     TTPVAIGISPHWMDRKGTLSLRKETFLNVVYDVCESLKTHGVKNILVLNGHGGNEAPL
                     RGAIPEFRSKLGLNLQFDSYWAAYTPEIVKKYMESGVVPGHSAEFETSFAMAAFPERI
                     HWEGVDYEKVKPHLGIKDPESRRQDEQFAREAKLATTAKGEAMIAVAVEWTAARLERM
                     IAAE"
     gene            1533..2459
                     /locus_tag="VLE22_15290"
     CDS             1533..2459
                     /locus_tag="VLE22_15290"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSB15820.1"
                     /translation="MDRRTFSRTLIALPAALHHAGAGESLFVSTPLTAEGSFTDGIEG
                     PACDAEGNVYAVSFARQPTIGKVTPDGKGEVWLEMPAGSLGNGIRFDRKGMMYVADYS
                     GHNILRVDPATRAVQVFAHNSSMSQPNDIAITRGGVLYASDPNWKEKTGQIWRIDTDG
                     STHQVAGAMGTTNGIEVSPDGRTLYVNESEQRNVWAFRIGPDGGLSGKRLLIQFPDHG
                     MDGMRCDVDGSLYITRYGKGVVAKVSVKGKVLHEVDVLGARPSNLCFGGPDGRTCYVT
                     EVEHRRLVQFRVDQPGLEWKLWQKARKPKAAR"
     gene            complement(2468..3547)
                     /locus_tag="VLE22_15295"
     CDS             complement(2468..3547)
                     /locus_tag="VLE22_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15821.1"
                     /translation="MSLPLEERLGRLAAWFQRRNDRPLIGFTLGSYYPLHRYRNGCRK
                     LPEGPVRPEDIVVNDYLDDTERLFRLHEEAGGDLIWSAAPFFGMPWVEAALGCGVVAD
                     HVTGSTRSTPPPSFTSNPAVPEFSQNDPWVAKMLAFIPALSDLSSGRYPVGVTLMRGI
                     SDLLSALYGGEAFVLRMIDAPEEVRGVAERLAHFWISFGRCLLDRLPLFQGGTGSMMY
                     SLWCPGKTIWFQEDAAALLSPALYDEFIYPADRAIVAAFEHTVVHLHPTRFIPTQQLI
                     ASDVDVIELHIDRDGPRAEALEKHYLAVLAEKPLLVWGDVTNADLEFLLTRLPHRGLA
                     VNVVVNSVEEAHAIWETAMLLQYSA"
     gene            complement(3636..4259)
                     /locus_tag="VLE22_15300"
     CDS             complement(3636..4259)
                     /locus_tag="VLE22_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15822.1"
                     /translation="MGFWVAVLCASGAPAQVFSIGVKGGTPLSTSTATGVVGGRGGLG
                     PFALNIRRYTIGPTFEVALPFQLRIEVDALYKRLDTTEHRFFNPSFGKIYRQAANTWE
                     FPMLLKRLWLRGPVRPFAVAGGAFRRINSLEGSTEQFTSGFDPPYSVSRYEVDYQLTE
                     GGIVIGSGVRLAAWRLQVSPEIRYTRWTALRLQPTQNQVEFLVGLTF"
     gene            4355..>4583
                     /locus_tag="VLE22_15305"
     CDS             4355..>4583
                     /locus_tag="VLE22_15305"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSB15823.1"
                     /translation="MELSTIPRLLGISVALALLAGAMNAAPPYAPSTESLRMHKAPQW
                     FEDAKFGVFIHWGPYAVPAYHEWYVAFMSPKS"
BASE COUNT          951 a         1414 c         1399 g          819 t
ORIGIN      
        1 actctcgttc tgcgtgggct gccaggccat aaaggttcgc ttcttgctct ggattctgta
       61 cagattgatg cggaattcac gtccgggttg gggagggcgt ggatcgatac tcgggaaggg
      121 gatcttcatt tccccgaacc acacccggcg actgcggtcg atgcgcgccg cggattccat
      181 gccggcgttc cacttatcat cgaagacgcc cggttcggag ttcttgttga cgtcgaggtc
      241 gatccattcg ccttgcggcg agagttcgaa ttccttgtag cggcggatgt ccttgaggtc
      301 ccagccgatg aacacctcgg cgacgtccag atcccaaagc ccccacgtat ccctcttcgt
      361 ctggggattc ggattcatat acaggtcgtt gtatgggcag gtgaagagaa tgtaaaggtt
      421 cgccatggtc cacattgagc gaacctccgt gcggaacgcc gggtatttct cgccaaagcg
      481 gccggtttcg aacgttagta ccggcgcgtt cttccagaat gaagctgttg ggtcggcggt
      541 ggcgggcgag tcggccttcg cgcggcggct cgtcatggtc ttcgaatccg cggcgccgag
      601 cgtcgccgca agaagccctg agaggaaagt gcgtcgtgtc gtcatgcggt tgtgtcagtc
      661 gggtatacta tcatgccctc atgacatcga cgatttcccg ccgccatctg tgccttggac
      721 tggccggctc cacaggggcc gtccaggcga gcgccacgat cgctcgcacg gcgtcaggct
      781 acacagaccc gcgcaaggtg ctcctgtggg agtccacacg taaggaagtg cgcgagctcc
      841 tggatgccgg tcagctcaag gccatgatta tcccgaccgc cagcaccgag cagcacaacg
      901 aacacctggc gatgattcac gacacggcca gcgtgactct cgtcgcgcag caggccgcgt
      961 tgcggctcta cccgcgggtg atggtcacca cgccggtcgc gatcggtatc tccccacatt
     1021 ggatggaccg caaaggcaca ctgagccttc gcaaggaaac gtttctgaac gtcgtctacg
     1081 acgtctgcga aagcctgaag acgcacggtg tgaagaacat cctcgtcctc aacgggcacg
     1141 gcggcaacga agcgccgctc cggggtgcaa tcccggagtt ccgctccaaa ctcggcttga
     1201 atcttcagtt tgactcctac tgggccgctt acacacctga aatcgtgaag aagtacatgg
     1261 aaagcggcgt cgttcccgga cattcggccg agttcgagac ctctttcgcc atggctgcgt
     1321 tccccgagcg cattcactgg gaaggcgtgg actacgagaa ggtaaaaccc cacctcggga
     1381 tcaaggaccc ggaatcacgc cggcaggacg aacagttcgc acgcgaagcg aagctcgcca
     1441 ccacggcaaa aggcgaagcg atgatcgccg tcgccgttga gtggacagcc gcccgcctcg
     1501 agcgcatgat cgccgcggaa taggacacca gaatggacag aagaactttc tcacgaaccc
     1561 tcattgcctt gcccgccgcg ctgcaccacg ccggcgcggg cgaatccctg ttcgtctcca
     1621 cccctttgac tgccgaaggc agtttcaccg acggcatcga gggtccggcc tgcgatgcgg
     1681 aaggaaacgt ttacgcggtc agcttcgcac ggcagcccac catcggtaag gtgacgcccg
     1741 atggaaaggg tgaagtctgg ctcgaaatgc ctgccggcag cctcggcaac ggcatccggt
     1801 tcgaccgtaa gggcatgatg tacgtagccg actacagcgg gcacaacatc cttcgcgtcg
     1861 acccggctac gcgcgcagtc caggtgtttg cccacaactc gtcgatgagt cagccgaacg
     1921 acatcgcgat cacccgtggc ggagtcctat acgcaagcga tccgaactgg aaggagaaaa
     1981 ccgggcagat ttggcgcatc gataccgatg gctccacaca ccaggtggcc ggcgccatgg
     2041 ggaccacaaa tggaatcgag gtgagtcccg acggcaggac tctctacgtg aatgaaagcg
     2101 aacagcgcaa cgtgtgggcg tttcggatcg gtccggacgg cggcctttcc ggcaagcgat
     2161 tgctgatcca gttcccggac cacggcatgg acggtatgcg ctgcgatgtc gacggaagcc
     2221 tttacatcac ccgttacggc aaaggcgtcg tggcaaaggt gtcagtgaag ggcaaagtgc
     2281 tccacgaggt cgacgtcctc ggcgcccggc cgagcaatct ctgcttcggc gggccggacg
     2341 gccgcacgtg ctacgtgacc gaagttgagc accggcgtct cgtacaattc cgtgtggacc
     2401 agcccgggct cgagtggaag ctctggcaga aagcacggaa accgaaggct gctcgctaaa
     2461 cggcacttca agcggaatac tgaagaagca tcgctgtctc ccagatagcg tgcgcctcct
     2521 ccacgctgtt cacgaccacg ttcaccgcga gcccgcgatg cggcagccgc gtcagaagga
     2581 actcgaggtc cgcattcgtc acgtcgcccc agacgagcaa cggcttctcc gcaaggaccg
     2641 ccaggtaatg tttctccagc gcttcggccc gcgggccgtc gcggtcgata tgcagttcga
     2701 taacgtccac gtcggaagca atcagttgtt gcgtcgggat gaagcgcgtc gggtggaggt
     2761 ggacgacggt gtgctcgaat gctgccacga tggcacgatc ggccggatag atgaactcgt
     2821 catagagggc cggcgaaagc agcgcggccg cgtcctcttg aaaccagatc gtcttgccgg
     2881 gacaccacaa cgaatacatc atgcttcccg tgccgccctg gaacagaggc agacggtcga
     2941 gcaggcagcg tccgaacgag atccagaagt gcgcgagcct ctccgccact ccgcggacct
     3001 cctccggcgc atcgatcatc ctcaggacaa aggcctcccc accgtaaagg gcagacagca
     3061 gatccgagat gccgcgcatc agagtcacgc ccacggggta acgtcccgag ctgaggtcgc
     3121 tcagagcggg aatgaacgcg agcatcttcg ccacccacgg atcgttttgt gagaattcgg
     3181 gtacggcggg attggaggtg aaggagggcg gtggggttga gcgtgtggaa ccggtaacat
     3241 gatcggcgac aacaccacag ccgagcgcag cctccaccca cggcatgccg aagaacggcg
     3301 ccgcggacca gatcaggtcg ccgcccgcct cctcgtgcag acggaacagc cgttccgtgt
     3361 cgtcaagata atcattaacc acgatgtctt cgggccggac cggcccttcg ggcaacttcc
     3421 ggcagccgtt ccgatagcgg tgcagaggat agtaggaacc gagagtgaat ccgatcagcg
     3481 ggcggtcgtt gcggcgctgg aaccacgcgg ccaggcggcc gagccgttcc tcgagaggca
     3541 aactcatcac aagctccgaa tgggagtata catcgtcacc cggagcccgc ccggtgtcgc
     3601 gagggaaccg tagatcagga tgtgacggat tggcgttaaa acgtcaagcc caccagaaac
     3661 tccacctggt tttgcgtggg ctggaggcgc agagcggtcc agcgcgtgta gcggatttcc
     3721 ggcgagacct ggagacgcca tgccgcaagc ctcacgcccg acccgatcac gatgccgccc
     3781 tcggtgagct gataatcgac ctcgtatcgg ctcacggaat aaggcggatc aaaacccgag
     3841 gtgaactgtt cggtgcttcc ttccagcgag ttgatgcgcc gaaacgcgcc gcctgcgact
     3901 gcgaatggcc gcacgggtcc acgcagccaa agcctcttca acaacatcgg aaactcccag
     3961 gtattcgccg cctgtcggta aatcttaccg aacgacggat tgaagaacct gtgctcggtc
     4021 gtatcgagcc gcttgtagag ggcgtccact tcgatcctta gctgaaacgg tagtgcaacc
     4081 tcgaaggtcg gcccgatcgt gtagcggcga atgttcaacg cgaacggacc gaggccgcct
     4141 cggccgccaa ccacgcccgt ggctgtcgag gtcgagaggg gtgtcccacc tttcacgccg
     4201 atggagaaaa cctgagcggg tgcgcccgaa gcgcagagaa ccgcaaccca gaatcccatg
     4261 gacatccagg ccttatccac gtttctgata ttagcatctg atttttctcg cccggcgcgt
     4321 gaattgtgta tactcttcgc cggatcacca agccatggaa ctgagtacga ttccacgcct
     4381 cctcggtatc agcgttgcgc tcgcgttgct cgcgggcgcg atgaacgccg caccgcccta
     4441 cgcgccgtcc accgaatctc tgcggatgca caaggcgccg caatggttcg aagacgccaa
     4501 gttcggagtc ttcatccact ggggtcctta cgcggtgccg gcctaccacg agtggtacgt
     4561 cgcgttcatg agtccgaagt cag
//
LOCUS       DATIFQ010000332        14682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1835331, whole genome shotgun sequence.
ACCESSION   DATIFQ010000332 DATIFQ010000000
VERSION     DATIFQ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14682
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835331"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VLE22_15310"
     CDS             <1..681
                     /locus_tag="VLE22_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15824.1"
                     /translation="DIDPDYDDGFQNGIHPLFYKAGQTKPGPDEWGSIGAWAWGLSRA
                     MDYFETDKAIDAKRVAVMGHSRLGKTALWAGAQDTRFALVISNDSGCGGAALSRRKFG
                     ETVKIINTAFPHWFCDNFNRYNDKEDELPVDQHMLIALMAPRPVYVASAEDDKWADPR
                     GEFLSALHADPVYRLLGTDGVPAKEMPAVNQPVMGTIGYHIRSGKHDVTDFDWEQYLN
                     FADKQFRR"
     gene            692..2755
                     /locus_tag="VLE22_15315"
     CDS             692..2755
                     /locus_tag="VLE22_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSB15825.1"
                     /translation="MRAIALLCALTSLHAQESLNVTSGLGGMVDKYLTPIAHKHLETR
                     AARISKISSPAEVAERQRYIRERLLDQIGGFPEKTPLNPKIIGTLERDGYRVEKLLYE
                     SLPRYYVTANVYVPTNAKPPFAAVLGTAGHSNEGKAYSLYQRVWIALAKRGFLVLAYD
                     PPGQGERSEYWDSELGRSQVGGPTSEHSMAGIQCLLTGTNIARYFIWDGIRAVDYLLT
                     RQDVDPKRIAVTGNSGGGTQSSYLAALEPRLAAAAPSCYLTSWEKQWMDPGPQDAEQD
                     FVNFLKDGLDFGDFLISFAPKPIKMMTAIRDFFPIDGARATFAEAHRIFEVMGQGDRI
                     GFFEYDDTHGWSKPRREATYRWLEQSLRQRTDEGIEPEFDTEPAVNLNCTATGQLSTS
                     LKGETVQSLNLALAERTYRPKTPPDIAGRLSLTVQRGVPAARRYGEVGRDGYRIEKIA
                     LDTEPGIVVPALVFVPASGEARKPAVLFVNPAGKSADGGAGGDLEALVRAGNVVLSVD
                     PRGWGESAGAAGRSGYRASFQTAMRALLVGKTMAGMQVGDLLRAFDYLVSMPDVDASR
                     VSIIGKGNGGVLALYAAALEQRIQKVACEGSVVSYMDIVRARIHDGMMDIIIPGVLRE
                     FDLPDVTRTIAPRTVWLVSPRTPAGAPAPMEHARAQYGTQQGLRVVERPEGFSFGKVY
                     AEWLR"
     gene            2752..3825
                     /locus_tag="VLE22_15320"
     CDS             2752..3825
                     /locus_tag="VLE22_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15826.1"
                     /translation="MTPINQGLLTQTRESIVRGLRWLRQHDAEIAQIQDLSAHYKAPY
                     LYAAVGDSVLGGRYAHRIGALYLQPDGDFRTGPADKGWSHLPCSPANRYLYSNGWIIA
                     GLRRLGYYGAAVRGIDFVRRFQSAELGGFASRFDPATGHIEQRYLDSSSTSSAGLALL
                     ACGRVEEAARAGDFLLRLIDAQPHPDRFYYCSWETGAGLVTDVWGDEDPNSVRGRKQF
                     CLSAEADPRYELTWLIGKPMKFLARLYDQTGDRKYLEGSTTLFNFFHNLGDERWHNYA
                     SCKTMWASAELYRHTGEPRFAETAGRILSWFCESQLAGGLWVHYLWYRDEREQPLQAA
                     LDLVQELCAEMSDVVFDLSAAGV"
     gene            3848..5542
                     /locus_tag="VLE22_15325"
     CDS             3848..5542
                     /locus_tag="VLE22_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSB15827.1"
                     /translation="MDVSRLDLIVLVGYLAAVTLVGLLGSLRVRGTKDYFLGGRRFGK
                     VLMVGQNFSVGTHPEMIVSVAGVVYATGLSGIWYQWKNLFATPFYWMMAPVFRRARRT
                     TVAEMVEDRYGPWMAGIYTAYALTYFTINIASLLKGAAKVINQAVGGYVGVNHVVVAM
                     TVIFMLYSFIGGKVASVWNTVLQGFLILTLSFMLIPLAWPHVGGISGMSAVLDPHRLS
                     IVTPAGVGAWTILMLTLNGLIGIMAMPHQMGVIATGKDEHSCRIGFFAGNYVKRICTA
                     GWATVGLVAAALAARGFFGPQQFPDPEDIFGALCKRLLFPGLLGLLTACILASCMAAC
                     AAFMVDSGALFTQNLYRRYWVRARPDNHYLWVGRISGFVITLLGVCYALFLVKRVLYS
                     FLLTETMSTYMGISFLGGILWRRANRWGALASLVTAFSVNFLLYYVRGERIDHWDANV
                     FLAALVSGAVTLVVVSLLTGHEPDARLNSFFGRAQTPAGAPESDSATYVDPAPKRVVA
                     EAGLQSLLVNLLQPIRAACGVGFLRAYRADLTGTVASLALILALVAAVWLIFSMPV"
     gene            complement(5557..6600)
                     /locus_tag="VLE22_15330"
     CDS             complement(5557..6600)
                     /locus_tag="VLE22_15330"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB15828.1"
                     /translation="MWRILVFLLAGVLPAQQYPVEPYPDNTERREGDNGFSRGFGMTN
                     HAYSFDWFDSHAHLAYSHFPTRLRGEQIQEVMDRWFRMTGMYQSGRAILMDPYLETME
                     WAKNDPRVYVFWWLKWEQSDQLPEIQRRVKEGLVQGLKLHSGDFRKNPNPDYRAMGTP
                     GWHAIYAYCEQAELPIVLHLNQHWGDQRYTYGKGSKEFWAEAGYTNQQLLDYFLNEMA
                     VKHPKVKWVLTHMNFQGNESLARLFDRYPNLYVDTSIGMFLREFDMLSPEEIKPFRDF
                     CIKYADRLMFGTDAFAYQPLESAYPDHVRNWWLPHQLFIMQLRLPQKTLEQITHGTVE
                     ALLGKYAKRVVKR"
     gene            7378..7620
                     /gene="acpP"
                     /locus_tag="VLE22_15335"
     CDS             7378..7620
                     /gene="acpP"
                     /locus_tag="VLE22_15335"
                     /inference="COORDINATES: protein motif:HMM:NF002151.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSB15829.1"
                     /translation="MSSVEQKVKQIIVEQLGVDEGQVDGTASFVDDLGADSLDIVELV
                     MAFEEAFELDIPDEDAEKIRTVKDAVDYIEVKAAKK"
     gene            7661..8905
                     /gene="fabF"
                     /locus_tag="VLE22_15340"
     CDS             7661..8905
                     /gene="fabF"
                     /locus_tag="VLE22_15340"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF004970.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HSB15830.1"
                     /translation="MSRRVVVTGIGLVTPVGVGTEESWRALCSARSGIKNITAFDATA
                     FACRIAGEIRDFSPEQFIERKELKKMGRFIQFAIAASDFAVASAGLKTAEEDQERVGV
                     YIGSGIGGFEIIEREHKNLIEKGPGRISPFFIPATIVNLASGHVSIRFGAKGPNSATA
                     TACTTGAHAIGDSYRIIQRGDADVMICGGAEAPVCPMSIGGFAAMRALSTRNDAPERA
                     SRPWDKDRDGFVVGEGAGILILEELEWAKRRGAKILAELVGYGMSGDAHHITAPSEDG
                     DGAFRVMRNALRDAGLQPSQIDYINAHGTSTDVGDRIETAAVKKCFGDHSYKVAVSST
                     KSMTGHLLGGAGGIEAGITVLAIRDQIAPPTINHEVPDPDCDLDYVPNQARPMKIEYA
                     LSNSFGFGGTNGCLIFKRYAGD"
     gene            8970..9740
                     /locus_tag="VLE22_15345"
     CDS             8970..9740
                     /locus_tag="VLE22_15345"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HSB15831.1"
                     /translation="MKIAVSVKQVPARDSVLRPDSSARWIQEGDLSFEINEPDAYALE
                     EGLQLKEKYGGEVLVVCIGPARAAQTIREALAKGADRAIHVEEDNLGAIDTLGVARLL
                     AAAVGSESPDLILTGLQSDDLGAGQTGVVLAELLGLPHATIIMQVEKLDGTLRVKREL
                     EGGWFQRIEMPMPAVLTIQSGISKLRYATLMGIKKARTKEVRRLTETDLGVDITPQVE
                     IQNVYQPRHSKQVQVLEGPAKDVAAKLVEKLRFEARVL"
     gene            9737..10723
                     /locus_tag="VLE22_15350"
     CDS             9737..10723
                     /locus_tag="VLE22_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HSB15832.1"
                     /translation="MSRILVVLEQQGDSWHRLSWEALAAGQQMAADLSWPVSAAVLGN
                     AASERASKATKWKLDSVYAVEHSLLSTYTADGFTAALEGLIRKIQPSLVLFPHTYQAR
                     DYAPKLATRFGRVLVSDIIQHAVVDGVPVFVRQLFQGKLSADIRCTGPAPYFVSVQAG
                     AYRSDSLEAGFAQVETFIPELDDAVIRVTPLEPFRESAGAVDLSNAEVIVSVGRGIKE
                     KEHLQIIEKLAEALGAELGASRPICDNGWLPMERQVGSSGQTVSPKLYVAIGISGAIQ
                     HLVGMKGSKTIVAVNKDPEAPIFEVADYGIVGDLFEVVPALTEEIRKAKGSG"
     gene            complement(10720..11691)
                     /gene="cas6"
                     /locus_tag="VLE22_15355"
     CDS             complement(10720..11691)
                     /gene="cas6"
                     /locus_tag="VLE22_15355"
                     /inference="COORDINATES: protein motif:HMM:NF021551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HSB15833.1"
                     /translation="MTGAPLVRMPTFEFYAYRLRFTAADPLRFPAGGAGNTVRGAFGT
                     ILRRIVCVPDCVDAKTCAIRTTCPYARIFEPTAAAGPGPSGLKDWPRPFVLRAHHLDG
                     RTLVPGERFHFDLHIFDLRDPAVAYFALTFAQLAKEGFGTGRGRARLETVDQLRLDST
                     AAARIYEGATLVVRDPVEPLVLSLAPANDAVTRVRVRFVTPTELKSAGDITPRPEFQT
                     LFGRIRDRISALRALYGPGPLEIDFRGMGERASQVRMSRCEIRHVESARRSTRTGQTH
                     PLGGFTGEAEYEGELAESLPYLHAAHWTGIGRQTVWGKGVIEVQTDY"
     gene            complement(11713..12117)
                     /locus_tag="VLE22_15360"
     CDS             complement(11713..12117)
                     /locus_tag="VLE22_15360"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSB15834.1"
                     /translation="MKGTWVSDSRWQQGGSYRRVLLDKKAEVLAKLGGKFDTLAAMGR
                     VAEDDQASISHDEFVLLSLNSLDYDKLRLVEEALERLRHGGFGVCQYCGEPISKKRLQ
                     AIPWARNCIACQNLLAALSPDVQEPKAISPAL"
     gene            complement(12283..13557)
                     /locus_tag="VLE22_15365"
     CDS             complement(12283..13557)
                     /locus_tag="VLE22_15365"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HSB15835.1"
                     /translation="MRFLLRLIDPVVSFQENQPPSISLRPSVRALVAAVLLTAPAGLP
                     GAEISERARRLHEEAFVFDGHVHMITRQFYHGGNIGDRVQDGQVDLPRIKEGGVDALC
                     FTVYVTEKYYPPRYETRQALRLMDLALEQIEKNKDRIELALNASEIERINRSGKIAAV
                     LDLEGSADLDGDLGVLRDLYRLGLRVIQLPAHNWTSNYADSCCAPPKWGGLNERGRAL
                     VREMNRLGMMINISHASDQTVEQAIDLSTDPIVATHHGLRSLNDIPRNMPDGLMKKLA
                     AKGGVIGFHIGNEFHNRPMFEWRTKQQKKAFWDTSAIGRKEAALSIEEIDRIVAPEFP
                     MVGMNPPDELMFSVDQWIDVVDRAIQIVGEDHVSLGSDFDGGPTPPRGMRDIRDLPML
                     TDAMLRRGYSESRIRKFLGGNLMRVFRQVTER"
     gene            13556..>14682
                     /locus_tag="VLE22_15370"
     CDS             13556..>14682
                     /locus_tag="VLE22_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15836.1"
                     /translation="MALPVIILFCLACCALAPAQQRGWWPQSYKVDRDEAAGTLVLST
                     PYYTFEHDLKKGGALSRITLTHGRARNLLVSPVESRIRLMVRPEGLQDAQARRRAPGA
                     TFSDLRDNAPKVTHTKSGKAEVVTVEASLLSENGKNSGARVRTTYNYRWGYVKVRKQF
                     ILDSPTDLVSLTALTALLDPSLSDYGWRPAAMEENGNSPFNWTNGQIRGWGKMRPGTH
                     MDLPFRTRFVPRYLVFANHGIEGLEWFVTDDLHQWDYQMTGQPGTGYCEIGASNEPLG
                     VRLNIEPMSLPWNPLLAKGGYLKLKGTYTFDYYLGVPVLEGHAQKLWLHESVRGIRNR
                     DGKEITEGEIRKLADSGIREMTLHNDGDAFQDGIFWRDGSYP"
BASE COUNT         2792 a         4494 c         4456 g         2940 t
ORIGIN      
        1 gacatcgatc ccgactacga cgacggtttc cagaatggca tccatccgct gttctacaaa
       61 gcgggccaga caaagccggg acccgacgag tggggctcga tcggcgcctg ggcgtgggga
      121 ctgagccgtg cgatggacta cttcgagacg gacaaggcaa tcgacgcaaa gcgcgtcgcc
      181 gtgatgggcc actcgcggct cggcaagacg gccctctggg cgggtgcgca ggacacgagg
      241 ttcgcattgg tgatctccaa cgattccgga tgcggaggag cggcgctcag ccggaggaaa
      301 ttcggggaaa cggtgaagat catcaacacc gcctttccac actggttctg tgacaatttc
      361 aaccgctaca acgacaagga ggacgagttg ccggtcgacc agcacatgct gatcgcacta
      421 atggcgccgc ggccggttta cgtggccagc gcggaggatg acaagtgggc cgatccccgg
      481 ggcgagtttc tctctgccct acacgccgac cccgtgtacc ggctgctcgg aaccgatggt
      541 gtgccggcga aggaaatgcc ggcagtcaac cagcctgtga tgggtacgat cgggtaccac
      601 atccgctccg gtaaacacga cgtcaccgat ttcgattggg agcagtatct caatttcgcc
      661 gacaagcaat tccggcggta ggattttcaa aatgagagcc atcgcccttc tctgtgccct
      721 gacgagcctc cacgctcagg aatcgctcaa tgtcacatcg ggcttgggcg gcatggtgga
      781 caagtacctg acgcccatcg cgcacaagca tctggagacc cgggcggcac gtatctcgaa
      841 aatctcatcg cctgcggagg tggccgagcg ccagcgctac atccgagaac gtttgctcga
      901 tcagatcggc gggtttccgg agaagacgcc gctgaacccg aagatcatag gcacgctcga
      961 acgagacggc taccgcgttg aaaagctgtt gtatgagagc ctgccgcgat actacgtgac
     1021 ggccaacgtc tacgttccaa ccaacgccaa gccgccattt gctgccgtac ttggcacggc
     1081 ggggcatagc aacgagggca aggcctattc cctgtaccag cgagtctgga tcgcacttgc
     1141 caagcgggga tttctcgttt tggcgtacga tccgcccggt cagggcgagc gctcggagta
     1201 ttgggattcg gaactcggcc gttcgcaggt cggtggcccg accagcgagc actccatggc
     1261 tggcattcag tgcctgctta caggcacgaa catcgcccgc tacttcattt gggacggcat
     1321 ccgagccgta gactatctgc tcacgcgcca agacgtcgat cccaagcgga tcgccgtaac
     1381 gggaaactcg ggcggaggca cccaatcttc ctacctggcg gcgctcgaac ctcggcttgc
     1441 cgccgcagcg ccgtcctgtt acctcacgtc ctgggagaag cagtggatgg atcccggccc
     1501 gcaggacgcc gaacaggatt tcgtcaactt cctcaaagac ggcctggact tcggtgattt
     1561 cctgatctca ttcgcaccca agccgatcaa gatgatgacc gcgatccgcg acttcttccc
     1621 gatcgacggt gcgcgcgcca cgttcgccga agctcaccgc atcttcgagg tcatggggca
     1681 gggggacagg atcggctttt ttgaatacga cgacacacat gggtggtcga aaccccggcg
     1741 cgaggccacg taccgctggt tggaacagtc gctgcgtcag cgcacggatg aaggcatcga
     1801 gcccgagttt gacaccgagc ccgccgtgaa tctcaattgc acagccacag gtcaactttc
     1861 gacgtcgctg aagggtgaaa cagtacagtc gctcaacctg gctttggctg aacgtacgta
     1921 ccggccgaag acgcctccgg acatcgccgg ccgcctcagc ctgacggttc agcgcggcgt
     1981 cccggccgcg cgccggtacg gcgaagtggg ccgcgacggc tatcgcatcg agaagatcgc
     2041 actggacacc gagccaggaa tcgtcgttcc ggcgcttgtt ttcgttccgg cgtcgggcga
     2101 ggcgcgcaag ccagccgtgc tgttcgtgaa tccggcgggg aagtcggcgg acggcggcgc
     2161 cggcggcgat ctcgaagccc tggtgcgggc ggggaatgtc gtgctctctg tcgacccgcg
     2221 gggctggggc gaaagtgcgg gcgcggcggg ccgctccggc tatcgagcgt cgtttcaaac
     2281 cgccatgcgc gctctgctgg tgggcaagac catggccggt atgcaggtgg gagacctgct
     2341 ccgtgcgttc gactatctgg tttccatgcc cgacgtggac gcgtcgcggg tgtcgattat
     2401 cgggaagggg aacggaggag tgctggcgct atacgccgcg gctctggaac agcgcatcca
     2461 gaaggtagct tgcgagggct cagtggtgtc ctacatggac atcgttcgcg cgcgaattca
     2521 tgacgggatg atggacatca tcatcccggg cgtcctccgc gaattcgatc tgcccgatgt
     2581 gacccggaca attgcgccac ggacggtctg gcttgtctcg ccacgcaccc cggcgggcgc
     2641 accggcgccg atggagcacg cgcgggccca gtacggaacc cagcagggcc tgcgcgtggt
     2701 cgaacggccc gagggattca gcttcggaaa ggtatatgcc gaatggctgc ggtgacacca
     2761 atcaatcagg gactcttgac ccagacgcgg gagtccatcg tacgcgggct gcgttggctg
     2821 cggcagcacg atgccgagat cgcgcaaatc caagacttga gtgcgcacta taaggcgcct
     2881 tacttgtatg cagcagtcgg tgattccgtc cttggcggtc gctacgcaca ccgaatcggc
     2941 gctctgtacc ttcagccgga cggcgacttc cggaccggac cggcggacaa gggctggtcc
     3001 cacttgccgt gcagccccgc gaaccgctat ctctactcga acggctggat cattgccggg
     3061 ctgcgccgtc tcggatacta cggcgccgct gtacgaggaa tcgatttcgt gcgccgcttt
     3121 caatccgctg agcttggggg ctttgcctcg cgattcgacc cagccacggg acacattgag
     3181 caacgttacc tggactcctc cagcacctcg tcggctggtc tcgccctgct agcctgtggc
     3241 cgtgtcgagg aagccgcacg ggcgggggac ttcctgctcc ggctcatcga tgctcagccg
     3301 catccggacc gcttctacta ctgcagttgg gaaaccggcg ccggcctggt gacggacgtc
     3361 tggggcgatg aggaccccaa ctctgtgcgt ggacgaaaac agttttgcct gagcgcggag
     3421 gccgatcctc gctatgagct gacgtggctc attggcaagc ctatgaagtt cctggcccgg
     3481 ctctacgacc agaccggcga ccggaagtat ctggagggtt cgacaactct cttcaacttc
     3541 ttccataacc tcggagacga acggtggcac aattacgcct cctgcaagac catgtgggct
     3601 tcggccgagc tttaccgcca cacgggtgag cctcgattcg ccgaaaccgc cgggcgaatc
     3661 ctgagctggt tctgcgaaag ccagcttgcc ggcggccttt gggttcacta cctctggtat
     3721 cgcgatgagc gggaacagcc tctccaggcg gctctcgatc tcgttcagga gctctgcgcc
     3781 gagatgagcg acgtcgtgtt tgatctcagc gcggcgggcg tgtagccgga cggatcttga
     3841 atctgctatg gacgtctccc ggttggacct tatcgtcctg gtcggctacc ttgccgcggt
     3901 gacattggtc gggctcctgg gcagcctccg cgtacgcgga acgaaggact acttcctcgg
     3961 agggcggcgc ttcggcaagg ttctgatggt gggtcagaac ttcagcgtcg ggacccaccc
     4021 ggagatgatc gtctcggtgg ccggcgtcgt ttacgcgacc ggtctgtccg ggatttggta
     4081 ccagtggaag aacctctttg ccacgccctt ctactggatg atggcgccgg tattccggcg
     4141 cgccaggcgc acgactgtcg cggaaatggt cgaggaccgt tatgggccct ggatggctgg
     4201 aatttacact gcctatgcgc tgacgtattt cacgatcaat atcgccagtc tgctgaaggg
     4261 ggcggccaag gtcatcaatc aggcggtcgg cggctacgtg ggagtgaacc acgtggtcgt
     4321 cgcgatgacg gtgatcttca tgctgtacag tttcatcggc gggaaggtcg cctcggtgtg
     4381 gaacacggtg ctccagggct tcctgatcct caccctttct ttcatgctga tccctctggc
     4441 gtggccgcac gtgggcggta tctcggggat gagcgccgtc ctcgatcctc accgcctttc
     4501 catcgtcact ccggccggtg taggcgcctg gaccatcctg atgctgactc tgaacggact
     4561 gatcggcatc atggcaatgc cgcaccagat gggagtcatc gccaccggaa aggacgagca
     4621 tagctgccgc atcgggttct tcgccggcaa ctacgtcaag cggatctgca ccgccggatg
     4681 ggcgacggtg gggctggtgg cggccgctct ggctgctcgt ggcttcttcg gcccgcagca
     4741 gtttcccgat cccgaggaca tcttcggtgc cctctgcaag cgtctcctgt tcccggggtt
     4801 gctcggcctg ctcactgcgt gcatcctcgc ttcctgcatg gccgcctgcg ccgcgttcat
     4861 ggtggatagc ggcgctttgt tcacccaaaa cctctatcgc cgctactggg taagagctcg
     4921 gccggacaac cactatctgt gggtggggcg gatcagcggc ttcgtcatca cgctgcttgg
     4981 tgtctgctac gcgctcttcc tggtcaagcg ggtgctctac tcgtttctcc tcaccgagac
     5041 gatgtccacg tacatgggga tcagttttct tggcgggatc ttgtggcggc gtgcaaaccg
     5101 atggggtgcg cttgcgagcc tcgtgacggc cttctcggtg aacttccttt tgtattatgt
     5161 gcggggagag cggatcgatc attgggatgc caacgtgttc ctggcggcgc tcgtgagtgg
     5221 ggcggtcacc ctggttgtgg ttagcctgct caccgggcat gagccggacg cgcgcctgaa
     5281 ctcgttcttt ggccgggcgc agacgccggc cggcgcacca gaaagtgact cggcaaccta
     5341 cgtcgatccg gctccgaagc gcgttgtggc ggaggcaggc cttcagtctc tcctggtgaa
     5401 cctgctacag cccatccgtg ccgcctgcgg cgtggggttc ctccgagcct accgcgccga
     5461 cctgaccggt accgtcgcca gtctcgccct catcctcgcc cttgtcgccg cagtttggtt
     5521 gatcttcagc atgccggttt gagggttccc tttcacctat cgttttacga ccctcttcgc
     5581 atactttccc agtaacgcct ccacggtgcc atgggtgatc tgttccagag ttttctgagg
     5641 caggcgaagc tgcatgatga acaactggtg cggcagccac cagttccgca catggtcagg
     5701 gtaggcactc tcgagcggct gataggcgaa cgcatcggtg ccgaacatca gccggtctgc
     5761 gtacttgatg cagaagtcgc ggaacggctt gatctcctcg ggcgacagca tgtcgaactc
     5821 gcggaggaac attccgatgc tggtatccac gtacaggttc gggtaacggt cgaacagccg
     5881 cgccagtgac tcattgccct ggaagttcat gtgtgtcagc acccacttca ctttcgggtg
     5941 cttcaccgcc atctcgttca gaaagtaatc gagcaactgc tggtttgtgt agcccgcctc
     6001 ggcccagaac tccttcgagc cttttccgta ggtatagcgc tggtcgcccc agtgctggtt
     6061 caagtggagc acgatcggca gttccgcttg ttcacagtag gcatagatcg cgtgccaccc
     6121 cggagttccc atggcgcggt aatccgggtt tgggttcttc cggaagtcgc cggaatgaag
     6181 cttcagaccc tgaaccagcc cttccttcac gcggcgctgg atctccggta actggtccga
     6241 ttgttcccac ttgagccacc agaaaacata gacgcgtggg tcgttctttg cccattccat
     6301 cgtttcgagg tagggatcca tcaggatggc ccgtccgctc tggtacatgc cggtcatgcg
     6361 gaaccaccgg tccatcactt cctggatctg ttccccgcgc agccgcgtgg gaaagtggct
     6421 gtatgccaga tgtgcgtggc tgtcgaacca gtcgaacgag taggcatggt tcgtcatgcc
     6481 gaagccgcgg gagaaaccgt tgtcaccctc cctgcgttct gtgttgtccg gatacggttc
     6541 cactggatac tgctgtgccg gcaacacacc ggctagtaga aatacgagta tcctccacat
     6601 gacgacctcc gccttatcgc ctctcgaaca cgatcacgcc atcctcaggc gctctgcctg
     6661 taaacaccgc gaagtccccg ggctacgtcc gcctgttgga taccgggcgt tgccggccga
     6721 tacgctgctc ctcccgagtc cagccacaca gtcatcggag agccctccgt agccctcagt
     6781 cgtaccttga gcgcgtcacg ggtttgctcc acagagccgc aatggaaaag tcagcatcag
     6841 gactcccttg ccgtcggtgt gccgcgcacc catagagtgt atctctgctc caggcccgaa
     6901 aggcggtcta cgaagcgagt tctgctatac ttccgcccat gctgaaacgc accagctttc
     6961 gtcccgaggt ggatcgctgg gttcacatca tttaccgtct ccgtccatga cgaaaacggc
     7021 cggttacttg cctcggagag ccggcccggc ctccccgcgc ccgtcgatct gctcccgaaa
     7081 gtggtgcgtg ttcgcttcaa cggaatccgt cttcgcaccg gattgaaaat ccgcggggcc
     7141 cagaacgggg gtgggtgagg agatctgcga cgagaacaac ctggcggccg ctcgcgatcg
     7201 ctaaaggaag agcgcacacg acagttgcgg aatgttctga tcgtcgctag aatcagttga
     7261 tttgtacaca gccccggctg gcatgatcac cgacaggcca gcgaatatga gattgtgtct
     7321 ctcagggaac cgccagcccg gaggctacag tggattcaag cgagtttggg aggattcatg
     7381 tcgtcagttg aacagaaggt gaagcagatt attgtcgagc agcttggcgt cgacgagggc
     7441 caggtcgatg ggaccgcctc attcgtcgat gatctgggcg ctgactcgct cgacatcgtc
     7501 gaactcgtga tggccttcga agaggctttc gagttggaca tccccgatga agacgccgag
     7561 aaaatccgca ccgtcaagga cgccgtcgac tacatcgaag tcaaggccgc caagaagtaa
     7621 cagcgacttc ccatgttagg cttttcaaaa agggggagat ttgtcgcgta gagttgtcgt
     7681 taccggcatt ggactggtga cacccgtggg cgtcggtacg gaggagtctt ggcgggcgct
     7741 atgcagcgcg cgttccggca tcaagaacat taccgcgttt gacgccacgg cctttgcctg
     7801 tcgtatcgcg ggggaaatcc gggacttcag tccggagcag ttcattgagc gcaaggaact
     7861 gaagaagatg ggccgcttca ttcagttcgc gatcgccgcc tctgatttcg cggttgcgtc
     7921 tgctgggctg aagacggccg aggaggatca agagcgggtc ggtgtctaca tcggcagtgg
     7981 catcggcgga tttgagatca tcgagcggga acacaagaac ctgatcgaga aagggccggg
     8041 ccgcatttcg cccttcttca tccccgctac catcgtgaac ctcgcctcgg gacacgtttc
     8101 cattcgcttc ggcgcgaaag ggccgaattc cgccaccgcc acggcctgta ccacgggtgc
     8161 acacgccatt ggggattcgt accggattat ccagcgcggc gacgcggacg tcatgatttg
     8221 tggcggcgcc gaagcgccag tctgtccgat gagcatcggc ggatttgccg ccatgagggc
     8281 gctctcgacg cgcaacgatg ctcccgagcg ggcaagccgg ccctgggaca aggatcgcga
     8341 cggctttgtg gtcggcgaag gcgcgggaat tcttattttg gaggagttgg agtgggccaa
     8401 acggcgtgga gcgaagatcc tcgcggagct tgtgggatat ggcatgagcg gcgatgcgca
     8461 ccacatcacc gcgccttccg aagatggcga cggcgccttc cgagtcatgc gcaacgccct
     8521 tcgtgacgcg ggcctccagc cgtctcagat cgactatata aacgcgcacg gcacttccac
     8581 cgacgtcggt gacaggatcg aaacggcggc cgtgaagaag tgtttcggtg atcactccta
     8641 caaagtggct gtcagttcca ccaagtcgat gaccggccac ttgttaggcg gcgcaggtgg
     8701 aatcgaggcg ggtattacgg ttctcgcgat tcgcgatcag atcgctccgc cgacgatcaa
     8761 tcacgaggtc cccgatccgg attgcgacct cgactacgtg ccgaaccagg cgcggcccat
     8821 gaaaattgag tacgcattat ccaactcgtt cggatttggc ggtacgaacg gctgcttgat
     8881 cttcaagcgg tacgccggcg attgaccgct gattcgtcct gaccgccgaa aacatggcgc
     8941 tccgggtcgc tggttgagtg gtgaaaccca tgaaaattgc cgtctccgtt aagcaggtcc
     9001 ctgcacgtga ttccgtcttg cggccggatt catctgcacg ttggattcag gaaggcgacc
     9061 tttcctttga aatcaacgag ccggacgcgt acgccctcga agaaggcctc cagcttaagg
     9121 agaagtacgg tggagaggtg ctcgttgtct gcatcgggcc cgcccgggcg gcccaaacga
     9181 tccgggaagc gctggccaaa ggcgcggacc gcgccatcca cgtcgaagag gacaacctcg
     9241 gcgccatcga cacgctcggt gtggcccgcc tgctggccgc cgccgtcgga tccgagagcc
     9301 ctgacctgat actcactggg cttcagtccg atgaccttgg cgcaggtcag acgggcgtcg
     9361 ttcttgccga gctgctcggc cttccacacg ccactatcat catgcaggtc gagaaactcg
     9421 acggcacatt gcgtgtgaag cgggaactgg agggcggttg gttccagcgg atcgagatgc
     9481 cgatgccagc ggtgctgacg attcagtccg gcatcagcaa actccgctac gcgaccctca
     9541 tggggatcaa gaaggccagg acgaaggagg tgcgccggct taccgagacg gatctcggcg
     9601 tggacatcac tccgcaagtc gaaatccaga atgtttacca accgcggcat tccaaacagg
     9661 ttcaggtgct ggaaggtccg gcgaaagacg tggcggcgaa gctggtcgag aaactcagat
     9721 tcgaggcccg agtgctatga gccgaatcct ggtggttttg gagcaacaag gcgattcatg
     9781 gcaccgcctc tcgtgggagg cgcttgccgc gggtcagcag atggccgctg acttgagctg
     9841 gcccgtatcg gccgcggttc tcggcaacgc cgcaagcgag cgagcgtcaa aggccacgaa
     9901 gtggaaattg gatagcgtct atgccgtcga gcactcgctg ctttcgacat atactgctga
     9961 cggtttcacg gctgctttgg agggcctaat ccggaaaata cagccttccc ttgtgctgtt
    10021 tccgcacacg tatcaggccc gggactatgc acccaagttg gccacgcgtt tcggccgggt
    10081 gctcgtgagt gacatcatcc agcatgcggt tgtggacggc gttccagttt ttgtccggca
    10141 gctttttcag ggaaaactga gtgctgacat ccgttgcacg ggtcccgctc cgtacttcgt
    10201 gtcagtgcag gcaggtgcct accgctcgga cagcctcgaa gccggttttg cgcaagtgga
    10261 gacattcata cccgaactcg acgatgccgt catccgcgtg acgccgctgg agccgttccg
    10321 tgaatccgct ggcgcggttg acctgtcgaa cgccgaggtg atcgtctctg tcggccgcgg
    10381 catcaaagag aaggagcacc tccagattat cgagaagctg gcagaggctc tcggcgccga
    10441 gcttggcgct tcccggccta tctgtgacaa cggttggctt ccgatggaac gtcaggtcgg
    10501 aagttcgggc cagactgtct caccgaagct gtacgtcgcg atcgggatct ctggtgcaat
    10561 ccaacacctc gtcgggatga agggctcgaa gaccatcgta gccgtgaaca aagacccgga
    10621 agctcccatc ttcgaggttg cggactacgg catcgtcggt gaccttttcg aggtcgttcc
    10681 cgcattgacc gaagagatcc gcaaagccaa aggttcaggc taatagtccg tctggacctc
    10741 gataacgcct ttgccccaga cggtctggcg gccaatcccc gtccagtgcg cggcatggag
    10801 ataaggcaga gattcagcca gttccccctc gtactcggcc tcgcccgtga agcctccgag
    10861 cgggtgggtt tgccccgtcc tcgtggaccg gcgtgctgac tcgacatggc ggatctcgca
    10921 gcggctcatc cgcacttggc tcgcccgctc acccatgccg cggaagtcaa tctctaaagg
    10981 ccccggtccg taaagagcgc gcaaggcgct gatgcggtcc cggatgcgcc cgaacagagt
    11041 ttggaattcg ggccggggcg tgatatcccc ggcgctcttc agttcggtag gcgtgacaaa
    11101 acgcaccctc actcgcgtca cggcgtcgtt tgccggagcg aggctgagga cgagcggttc
    11161 cacaggatcc cgaaccacta aagtcgctcc ttcgtagatc ctcgctgcgg ccgtgctgtc
    11221 cagccggagc tgatcgactg tctccagccg ggcgcgtccc cttccagtgc cgaatccttc
    11281 tttcgcgagt tgcgcgaacg tcagcgcaaa gtaagcgaca gccgggtcgc gtagatcgaa
    11341 gatgtgcagg tcgaagtgaa acctctcgcc gggaacgagc gtgcgcccgt caagatgatg
    11401 ggcgcgcaaa acgaaaggcc ggggccagtc cttcaacccg ctgggcccgg gccctgctgc
    11461 cgcggtcggt tcgaagattc gagcgtacgg gcatgtcgtc cgaattgcgc aggtcttcgc
    11521 atcgacgcag tctggaacgc acacgatcct gcggagaata gtgccaaacg cgcctcggac
    11581 cgtgttgccg gcgcccccgg ccgggaatcg caacggatcg gccgcagtga aacgcaggcg
    11641 gtacgcgtag aactcaaaag tgggcatccg caccagtgga gcaccggtca cccgaccaat
    11701 ctaccagtgc gctcacagtg ctggcgatat cgcttttggc tcttgaacat ccggggacag
    11761 tgccgccagg agattctggc acgcgatgca gtttcgtgcc cagggaatcg cctgcagtcg
    11821 ctttttcgag atcggttcgc cgcaatactg gcacacaccg aaaccgccgt gcctcagccg
    11881 ctccagcgcc tcctcgacga gtctgagctt gtcatagtcc aaactgttca ggctgagcag
    11941 aacgaattcg tcatgtgaga tcgacgcctg gtcatcctcg gcgacccgcc ccattgccgc
    12001 cagggtgtcg aactttccgc ccagcttcgc gagaacctcc gccttcttgt cgagcagcac
    12061 ccggcggtag gatcccccct gctgccacct gctgtcgctg acccatgtcc ccttcatgcc
    12121 accgcctcct tccacagcgt ccgttgactc ttgtcgggcc aaactgccgg cggcccttaa
    12181 taggtgacac aaaagtataa gaattctcgc gaaaaatgca agcccttttt gtagaaaaga
    12241 ctgacaaccg cgcaagcggt caagatcctt aaagctaacc gtctatcttt cagtaacttg
    12301 ccgaaagact ctcatcaggt tcccgcccag aaactttcgt atccgactct cggaatagcc
    12361 ccgtcttaac attgcatcgg tcagcatcgg caggtcccgg atgtcgcgca ttccgcgcgg
    12421 cggggtaggc cctccatcaa aatcgctgcc gagtgatacg tggtcctcgc cgacgatctg
    12481 gatcgcccga tcgaccacgt caatccactg gtccaccgaa aacatcagct catcgggggg
    12541 attcatgccc accatcggga actcgggcgc gacaatccgg tcgatctctt cgatcgaaag
    12601 agcggcctct ttccggccaa ttgcgctcgt gtcccagaac gccttcttct gctgcttcgt
    12661 ccgccattcg aacatcggcc ggttgtgaaa ctcgttgccg atgtgaaagc cgatgacgcc
    12721 gcctttcgcc gcaagcttct tcatcagccc gtcgggcata ttgcgcggga tgtcgttcag
    12781 gctccgcaga ccgtggtgag tggccacgat cggatctgta ctcaggtcga tcgcctgctc
    12841 gaccgtttgg tccgaagcgt gagaaatgtt gatcatcatc ccgagacggt tcatttcgcg
    12901 gacaagagcg cgcccacgct cattgaggcc gccccacttc ggcggcgcac agcaggagtc
    12961 ggcgtagttg ctcgtccagt tgtgcgccgg aagctggatg actcgcaggc caagccgata
    13021 cagatctcgc agaacgccaa gatcgccgtc cagatccgca ctgccctcca gatcgagcac
    13081 cgccgcgatc ttgcctgaac ggttgattcg ctcgatctct gaagcattca gggccagctc
    13141 gatccggtct ttgttcttct cgatttgttc cagtgcgaga tccatcagcc ggagcgcctg
    13201 ccttgtctcg taccgcggcg ggtagtactt ctcggtcacg tagacggtga aacagagagc
    13261 atctacgccg ccttctttga ttctagggag gtcgacctgt ccatcctgga cgcgatcgcc
    13321 gatgttcccg ccgtggtaga actgccgcgt gatcatgtga acgtgtccgt cgaagacgaa
    13381 cgcttcttcg tgcagcctgc gcgccctctc ggagatttcg gccccgggca gtccagccgg
    13441 cgccgtcaac agcacggcgg ccaccagcgc cctgacagac ggtcgcagtg atatgcttgg
    13501 cggttgattt tcctggaagg agacaaccgg atcgatgagt ctcagaagaa atcgcatggc
    13561 tctgcctgtc atcatactgt tctgtctggc ttgctgcgca ctcgcacccg cacagcaacg
    13621 cggctggtgg ccgcaatcct ataaggtcga ccgcgacgaa gccgccggca cacttgttct
    13681 ctcgacaccc tactacacct tcgagcacga cctgaagaaa ggtggagcgc tttcccgaat
    13741 cactctcacg cacggacgag ccaggaacct gctcgtcagc ccggtcgaga gtcgcatcag
    13801 gttgatggtc cggccggagg gcctacagga cgcacaggca cggagacgcg cgccaggagc
    13861 cacgttcagc gaccttcgtg acaacgcgcc gaaggttacc cacacgaaat ctggcaaagc
    13921 cgaggtcgta accgtcgaag cttccctgct gagcgagaac ggaaagaact ccggtgcgcg
    13981 cgttcggact acctataact accgttgggg gtacgtaaag gtccggaaac agttcatcct
    14041 cgattccccc acggacctcg tcagcctcac cgctctcact gccctgttgg accccagtct
    14101 ctccgactac ggatggcggc cggcggccat ggaggaaaac ggcaacagcc cgttcaactg
    14161 gaccaacggt cagatccgtg gctggggcaa gatgcgtccc ggcacccaca tggacctgcc
    14221 gtttcgaact cggttcgttc cccgctacct tgtctttgcc aaccacggaa tcgagggcct
    14281 cgagtggttc gtcaccgacg atcttcacca gtgggactac cagatgactg gccagcccgg
    14341 aacgggatac tgtgaaatcg gcgccagcaa cgaaccactc ggcgtgagac tcaacatcga
    14401 gccgatgagc ctgccctgga acccactgct ggcgaagggt ggttacctga agcttaaggg
    14461 aacctacacc ttcgactact acttgggcgt cccggttctc gaaggtcacg cgcagaaact
    14521 ttggctgcat gagtcggtcc gcggaatccg caatcgtgac ggcaaggaga tcacggaagg
    14581 cgagattcgg aagctggctg attccggtat ccgcgagatg acgctgcata acgacggcga
    14641 tgcgtttcaa gacggcatct tttggcgtga tggcagttat cc
//
LOCUS       DATIFQ010000333         5078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_110449, whole genome shotgun sequence.
ACCESSION   DATIFQ010000333 DATIFQ010000000
VERSION     DATIFQ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110449"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VLE22_15375"
     CDS             <1..879
                     /locus_tag="VLE22_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15837.1"
                     /translation="VLKNAGSTVEFILDRSFGNKWTRFDGPDRNSVLNIGHLFSFLEE
                     NPDVQALSSHQLRYPKPAREGYVFFDMFFVRDPIDRIYSMYKFFKRIPESDDPLSDLA
                     KANDLAGYIAGMLADFPHYINDMQVNCLANGGTYIRPPGKHDLDAATAVLEKAAVPCV
                     VDRFNQSWIAAQYFLRPVFPGIDCRYVPHNTSSEYNTTLEERLHRVRAACGDKLYNQL
                     LALNVLDTQLVDRVRAEVARRFHLVPDHKQRLERLESELRMILAEMDAKHNEQNVLNH
                     DRASVAEEEPEFSENR"
     gene            complement(902..2917)
                     /locus_tag="VLE22_15380"
     CDS             complement(902..2917)
                     /locus_tag="VLE22_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15838.1"
                     /translation="MKIRCVILSLAALGCLAGGPRVGVSGRRVEVSAEGQTLLASPPE
                     GLWSIACDWRDGWPADWRHAEPEQVSHAGEWLILEAKLEACGGTWLLRDAYRPKGDLV
                     QGIRRFAWKGPQAAKRVTLSVRFQAQAERSQILMPGIVYYGNPSGARSGKVPVYTGKP
                     GEESIFEEHRFAMPFAYAELGTPAGLRGAALHTEPSPVPFGNLRDQWWSLGVSAREGG
                     VDLLALSGPTASNGKRSVIKAIQPGFKLYNDTFLNVPPEAVIEKTFYLEAFPVAREGD
                     GFRRPVRTSLDLFRPYYVEDLPGFGEILRAKYRFALSRWHEGDGVAGFKKYPDRTFFV
                     MGWAGQAEAPGYALQVLAKGLGDPKALEMAQKSLNFASNAHFYERGFRTWYDFAKKEW
                     SHDEPLSQGQAMTSFARAIRVGRKGGLDTSRWEAFLRKAAEFHARRILAPDWHPLSTA
                     EGFFIAPLVECSKLFNSEEFRRAALKAGEHYAKRHLSMREPYWGGTLDAESEDKEGAW
                     AAFEGFLSLYELTKKPEHLEWAKHACDVVLSYVVVWDIDLPAGRLRDHRFRTRGWTVV
                     SPQNQHIDVYGVIMAPAVYRMGQLLQREDLKRLALVMYRSCGQIIDPYGSQGEQPHHT
                     NYAQRGDTSDIFGLRGGYREEWTVFWITAHFLNAGAQFAEMGVPVWD"
     gene            3030..4889
                     /locus_tag="VLE22_15385"
     CDS             3030..4889
                     /locus_tag="VLE22_15385"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HSB15839.1"
                     /translation="MSRSRSREFREQLAQRVLIADGAMGTMLYSKGVFINRCYDELNL
                     SAPALVRETHESYVKVGAEIIETNTFGANRPRLTSHGFADKLQAINEAGVRLAREAAS
                     DSAFVAGAVGPLGLRIEPLGPTSFAEARAMFREHIEALAEAGVDLLILETFSDLNELR
                     EAIFAAREVAGEEMVVVAQVTIDDYGNLPAGATTELFTRRLAEWPADVIGCNCSAGPK
                     VTFETIEKMQHFSSKPLSAMPNAGHPATVEGRKIYLCSPEYMAQYARRFLAAGVRILG
                     GCCGTTPEHIKEVHSEVRSLAPVHRRLSVTVEEPAAAAQAIPKVPVPQKSSLGAKLTE
                     GKFVAFVEILPPRGVDASKEIAGARMCSEHGIDCINVPDGPRASARMSAQITCQIIQQ
                     QAGIEAVLHFCCRDRNILGIQSELLGAHAAGIRNLICITGDPPRMGSYPSATAVFDVD
                     AIGLVNIVNNLNHGLDIGGNPMGSQTALLIGVGANPGALNLDEEIRRFDWKVQGGAEY
                     VVTQPVFDLNLLEGFLKRIEHARLPVITGIWPLVSYRNAEFMVNELRVPVPEEYMERM
                     RAAGTAEKAREQGVAIAREMVQRVRPMVQGVQLSAPFGRYQMAVEVAEAIGPR"
BASE COUNT          943 a         1708 c         1510 g          917 t
ORIGIN      
        1 gtcctgaaaa acgcaggctc gaccgtcgag ttcattctcg atcgcagctt cgggaacaaa
       61 tggactcggt tcgatgggcc agaccggaac tcggttctga atatcgggca cttgttctcg
      121 ttcctcgaag agaatccgga cgtgcaggca ctatccagcc accagcttcg ctatccgaag
      181 cccgcccgtg aggggtacgt ctttttcgac atgttcttcg tgcgcgatcc gatcgatcgg
      241 atctattcga tgtacaagtt cttcaagaga atccccgagt cggacgaccc gcttagcgat
      301 cttgccaagg ctaacgactt ggccggctac atcgctggga tgctggcgga ctttcctcac
      361 tacataaacg acatgcaggt gaattgcctc gcgaacggcg ggacgtatat ccgccctccc
      421 ggcaagcatg atctcgacgc cgctaccgct gttttggaga aagcagccgt cccctgcgtc
      481 gtggatcgat tcaaccaaag ctggatcgcc gcccagtatt tcctcaggcc ggtgttcccg
      541 ggtatcgatt gccggtatgt tccacacaac acatcctccg agtacaacac taccctggag
      601 gaacgcctac accgagtgcg cgccgcctgc ggagacaagc tctacaatca actgctggct
      661 ttgaacgtcc tggacacgca actggtggat cgcgtccgcg ccgaggtggc acggcggttc
      721 cacctggtgc ctgaccacaa gcaacggttg gaacggctcg aaagtgaact tcggatgatc
      781 ttggccgaaa tggacgctaa gcacaatgag cagaacgtcc tcaatcacga ccgcgcaagt
      841 gtcgcggagg aagagccgga gttctcagag aaccgttgag cggcgcgagt cccctggcgt
      901 cctaatccca caccggcacg cccatctcgg cgaactgtgc gccggcgttc aggaagtgcg
      961 ccgtgatcca gaacaccgtc cactcctccc ggtagccgcc gcgtaggccg aagatgtcgc
     1021 tcgtgtcgcc ccgctgcgca tagttggtgt ggtgaggctg ctcgccttga ctgccgtacg
     1081 ggtcgatgat ctgcccgcag gagcggtaca tcaccagcgc cagccgcttc agatcctccc
     1141 gctggagcag ttgtcccatg cgatacacgg ccggcgccat gatcacaccg tagacgtcga
     1201 tgtgctggtt ctgcggcgac actactgtcc atccgcgcgt ccggaagcgg tggtcgcgaa
     1261 gccgccccgc cggcaggtcg atgtcccaca caacgacgta gctcaacacg acgtcacagg
     1321 cgtgcttcgc ccactccaga tgttcgggtt tcttcgtcag ctcataaagg gagagaaaac
     1381 cttcgaacgc cgcccaggcg ccttctttgt cctcgctttc agcgtccagc gtcccgcccc
     1441 agtacggttc acgcatcgag aggtggcgct tcgcgtagtg ctctcccgcc ttcagcgccg
     1501 cgcgccggaa ctcctccgaa ttgaacagct tggagcactc gaccagaggc gcgatgaaga
     1561 acccctccgc ggtcgagagc gggtgccaat ccggagccag gatccgccgc gcgtggaatt
     1621 cggctgcctt ccgcaggaac gcctcccagc gcgaagtgtc gaggccgccc ttccgcccca
     1681 cgcggatcgc ccgggcgaag ctcgtcatgg cctggccctg cgagagcggc tcgtcgtggc
     1741 tccattcctt cttcgcgaag tcgtaccagg tgcggaatcc gcgctcataa aagtgcgcat
     1801 ttgatgcaaa attgagcgat ttttgcgcca tttcgagcgc tttcgggtca cccaaaccct
     1861 tcgccaggac ctgaagggca tacccgggcg cctccgcctg ccccgcccac cccatcacga
     1921 agaacgtccg gtccgggtac ttcttgaagc ccgccacgcc gtcgccctcg tgccaccgcg
     1981 acaacgcgaa gcggtacttc gcccgaagga tctccccgaa ccccggcaag tcctcgacgt
     2041 agtacggccg gaagagatcc agcgacgtcc ggaccggacg gcggaatccg tcgccctccc
     2101 gcgccaccgg gaacgcctcg aggtagaacg ttttctcgat gaccgcctcc ggaggcacgt
     2161 tcaaaaatgt gtcattatat agtttaaatc caggctggat cgccttgata acagaccgct
     2221 tgccgttcga tgcagtgggt ccggagagtg ccagcaggtc caccccgccc tcccgtgccg
     2281 acaccccgag cgaccaccac tgatcgcgca gattcccgaa cggaaccggc gagggctcgg
     2341 tgtgcagcgc cgcgccccgc agtccggccg gcgtgcccaa ctccgcgtaa gcgaatggca
     2401 tggcgaaacg gtgttcttcg aagatcgact cctcgcccgg cttgccggtg tataccggca
     2461 ctttaccgct gcgcgccccg gagggattgc cgtagtagac gatgcccggc atcaggatct
     2521 gtgatcgctc ggcctgagcc tgaaaccgga cggatagcgt cacccgtttc gctgcctgcg
     2581 gtcctttcca cgcgaaccgc cggattccct gtacgagatc gcccttgggc cggtaagcat
     2641 cacgcagcag ccatgttccg ccgcaagctt caagtttcgc ttcaaggatc aaccactcgc
     2701 cggcatggga cacttgttcc ggttccgcgt gccgccagtc cgccggccag ccgtcccgcc
     2761 agtcgcaggc gatcgaccac aggccctccg gaggcgatgc cagcagcgtc tgcccttccg
     2821 ctgagacctc cacccgtctc ccgctcaccc cgactcttgg tcccccggcg agacagccga
     2881 gtgccgccaa gctcaatatg acgcatcgaa tcttcatact ccccgtaacc ctatcatccg
     2941 ggagggattt tttcatcttc cgcgtttggc cgtccggcgt ccggtgcggt acaatcggcc
     3001 tgtaatctag aggttccccc aaaggcttca tgtctcgtag taggtcccgg gaattccggg
     3061 aacaactcgc ccaacgtgtc ctgatcgccg atggcgcgat ggggaccatg ctgtactcca
     3121 aaggcgtctt catcaaccgc tgctacgacg agctgaatct ctccgccccc gccctcgtcc
     3181 gggagactca cgagagttac gtcaaggtgg gggccgaaat catcgagacc aacacgttcg
     3241 gcgcgaaccg gccgcggctc acctcgcacg gcttcgccga caagctgcaa gcgatcaacg
     3301 aggccggagt gcgcctcgcc cgggaggcgg cgagcgattc cgctttcgtc gccggcgccg
     3361 tcggaccgct cgggctccgc atcgagccac tcgggccgac gtcgttcgcc gaggcgcgcg
     3421 cgatgtttcg cgaacacatc gaagcgctgg ccgaggcagg cgtggacctg ctgattctcg
     3481 agacgttctc cgacctgaac gagcttcgcg aagcgatttt cgcggcccgc gaagtcgccg
     3541 gcgaggaaat ggtcgtcgtc gcacaggtca ccatcgacga ttacggcaac ctgcccgccg
     3601 gcgccaccac cgagctgttc acccggaggc tcgccgaatg gccggccgac gtgatcggct
     3661 gcaactgctc ggccggtccg aaggtcacct tcgagaccat tgagaaaatg cagcacttct
     3721 cctcgaagcc gctgagcgcc atgccgaacg ccgggcatcc ggcgactgtc gaggggcgga
     3781 agatctacct gtgctctccg gagtacatgg cgcagtacgc gcggcggttt cttgcggcgg
     3841 gcgtgcggat cctcggtggt tgctgcggca cgacgccgga gcacatcaag gaagtgcact
     3901 ccgaagtccg ctcccttgcg ccggtgcacc gcaggctgtc ggtaaccgtc gaggagccgg
     3961 cggcggccgc gcaggcgatc cccaaagtgc cggtgccgca gaagtcgagt cttggcgcga
     4021 agctgaccga aggaaagttt gtcgccttcg tcgagattct gccgccgcgc ggagtggacg
     4081 cgagcaaaga gatcgccggc gcccgcatgt gcagcgaaca cggcatcgac tgcatcaacg
     4141 tgcccgacgg cccgcgcgcc agcgcgcgca tgagcgcgca aatcacttgc cagatcatcc
     4201 agcagcaggc tggaattgaa gcggtgcttc acttctgctg ccgcgaccgg aacatcctgg
     4261 gcatccagtc cgagttgctt ggcgcgcacg ccgccggcat ccgcaacctg atctgcatta
     4321 ccggcgatcc gccccgcatg ggctcctacc cttcggctac cgccgttttc gacgtcgacg
     4381 ccatcggact cgtcaacatc gtgaacaatc tgaaccatgg gctcgacatc ggcggtaacc
     4441 cgatgggctc gcagaccgcg ctgctcatcg gcgtcggcgc caatccgggt gcgctcaatc
     4501 tcgacgagga gatccggcgc ttcgattgga aggtgcaggg cggcgcggag tacgtggtca
     4561 cgcaaccggt tttcgacctg aacctgctcg aaggcttcct caaacggatc gagcacgcgc
     4621 ggctgccggt gattaccggc atctggccgc tggtcagcta tcggaatgcg gagttcatgg
     4681 tgaacgagtt gcgcgtgccg gttccggagg aatacatgga gcgtatgcgc gccgccggca
     4741 ccgcggaaaa ggcccgcgag caaggcgtgg cgatcgcccg cgagatggtg cagcgtgttc
     4801 gtccgatggt ccagggagtc cagttgagcg cgccattcgg ccgctatcag atggcggtcg
     4861 aagttgccga agccatcggt cctagataaa gtacgggatt gatcgtgccc accgacctga
     4921 tcgagatcga cagcgaaaac cccgacccca aggcgatcga acgggcctcc tccgcaatcc
     4981 ggcgcggccg cgttgtcgcc atcccgaccg acgcgctcta taccgtcgtc gccgacccgt
     5041 tcaatctgca cgcagtcggc ctcgtctttc gggccaag
//
LOCUS       DATIFQ010000334         3248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2793819, whole genome shotgun sequence.
ACCESSION   DATIFQ010000334 DATIFQ010000000
VERSION     DATIFQ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793819"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VLE22_15390"
     CDS             <1..581
                     /locus_tag="VLE22_15390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB15840.1"
                     /translation="ERRNAYYQAQSRATNRLHRLVERLLDFGRMEAGTFRYRPETVDL
                     GSLAHSVIDEFEHIAAMSGHEIRVTVEPELPRVKADPEALGQALWNLLDNAIKYSPGR
                     PVVSLEVAREDGSVAILVRDHGLGIPVQEQKRLFRKFVRGSAAELAQVKGTGIGLAMV
                     DYIVHAHQGRIRLESVPGEGSAFTILLKVEPT"
     gene            578..1252
                     /locus_tag="VLE22_15395"
     CDS             578..1252
                     /locus_tag="VLE22_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB15841.1"
                     /translation="MSRILIVEDETDLAFGLEDDLKVEGYQVEIARDGETAGRRAREE
                     MWDLILLDIMLPRKDGFEVCRELRRAGVRTPIIMLTAKAQEAEKILGLELGADDYVTK
                     PFGPRELRARIKAVLRRTEEPVSDVYRFGDVEVDFARGEIHRGGMAVDVTPLELKLLK
                     AFVASRGRILSRELLLDAAWGTQTFVTDRVVDGHIVSLRRKIEPEPSKPRYLISVRGL
                     GYRFDG"
     gene            1394..3208
                     /locus_tag="VLE22_15400"
     CDS             1394..3208
                     /locus_tag="VLE22_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15842.1"
                     /translation="MTDNTVRNSNVETPLESWKEVAAYLQRDVRTVIRWEKSEGLPVH
                     RHHHLSRSSVYAYPSELDAWRANRKPLTEITRSTWRQPMPAFASVVVIALSLMMVGSG
                     PYAGSLVEAADGIVTRRIWKADLNLDGAPSPDGRYLAFDDETGNLAVRDLTTGEVRRL
                     TNGAGGWRYAAAPVFSPDAERVAFGWRNRPDTCELRVAAMDSSEPRVLYRAEGQEVTP
                     TDWSLNGSSLLVLRRREPSHSEIVTVAVADGSLNVLKSFDGASPDVRRLRISPDGRFV
                     AYDLPVDKAGNRDIFVLTVAGGQVSPLVQHTAQEQFLGWTPDGRSVLFLSDRTGNWGA
                     WLIPVANGKPQAEPTLVKRDAGAMEPLGFKLRDLRWLQWSPRGNSLLVRGRGGLYTID
                     ARTEAVSRAPWWPPREYGSYIIDPCWSADEKAVYYVEPRVLAPGGQTRLLRADLVTGR
                     EVLIHLFPKTVTGGAVLLSPNGDWFLLWLLHGTGWSPAVMPANGTEVRDLPGPWSMPQ
                     VVPRSLTWGPHSNQVVFIKHPGTPPRIATSELWGVPAESGEQRSLGFSVGKDVQFPVS
                     IHPDGKRLAFTAWEPATEIWVMENFLLPSSKSGNSSAE"
BASE COUNT          656 a          895 c         1065 g          632 t
ORIGIN      
        1 aggaacggcg aaacgcttac tatcaggcgc agtcgcgtgc cacgaaccgc ttgcatcggc
       61 ttgttgagcg cctcctggat ttcggcagga tggaagcggg cacctttcgt taccgcccgg
      121 aaacggtaga cctcggcagc ctcgcgcact cggtcatcga cgagtttgaa cacatcgccg
      181 cgatgagtgg gcacgagatc cgagtcactg tcgagcctga attgccacgt gtgaaggcag
      241 atccggaagc actcggccaa gccctttgga acctcctgga caacgcaatc aaatactccc
      301 ctggccggcc ggtcgtttcg ttagaggtag cccgggaaga tggctctgtt gcgatcctgg
      361 tccgtgatca tggactcgga attcccgtcc aggaacagaa gcgcctgttc cggaagttcg
      421 ttcgtgggtc ggcagccgaa ttagcccagg tcaagggcac tggcattggt ctggcaatgg
      481 tggattacat cgttcacgcc caccagggcc gcatccgtct ggaaagcgtg cccggcgaag
      541 gcagcgcgtt taccatactg ctgaaggtgg agccaacgtg agccgcatat tgatcgtcga
      601 ggacgaaacc gaccttgcct tcggccttga agacgactta aaggtcgagg gctatcaggt
      661 cgaaatcgca cgcgatggtg agaccgccgg ccggcgcgca cgagaggaga tgtgggacct
      721 catcctgctg gacatcatgc tgccccgcaa agacggcttc gaggtctgcc gtgaactgcg
      781 tcgcgccggc gtgcgcacgc cgatcatcat gttgacggcg aaggcacaag aagccgaaaa
      841 aatcctgggg ctcgaactcg gcgcagacga ttatgtgaca aagccgttcg gtccccgaga
      901 actgcgggcg cggatcaaag ccgtgctacg gcggacggag gagccggttt ctgatgtcta
      961 ccgcttcgga gatgtggagg tggacttcgc ccgaggcgaa atccaccgcg gcgggatggc
     1021 tgtcgacgtc actccgctcg aactgaaatt gcttaaagct tttgtggcga gccgtgggcg
     1081 cattttgagc cgtgagctgt tgctggacgc tgcctggggc acacagacct tcgtcaccga
     1141 ccgtgtcgtg gacggtcaca tcgtcagcct gagaaggaaa atcgagccgg agccgtccaa
     1201 gccgcgatat ctgatcagcg tgcgtggcct gggttaccgg ttcgatggat gagattgcgt
     1261 tgtgagagta gacacttaat gacgcaggct gtatggcatg ttatgtcaat tgcaggcgaa
     1321 gaggatctga aataggtttg tgatggcgga acctagaaaa cagaaaacga ggttacccga
     1381 aaggaggttt gccatgacag acaatactgt aagaaactcg aacgttgaaa cgccgctgga
     1441 gtcctggaag gaggtggcgg cttaccttca gcgcgatgtc aggacggtca tccgctggga
     1501 gaagtcggaa ggactgccgg tacatcgcca tcaccacctt tcccgcagca gcgtctacgc
     1561 ctatccgagc gaactggatg cttggcgcgc gaatcgaaaa ccacttacag aaataacacg
     1621 ttcgacttgg cggcagccta tgccggcctt cgcctccgtc gtcgttattg cactgtcgct
     1681 gatgatggtc gggagcggcc cgtacgctgg ttcgctcgtc gaggcggcag acggcatcgt
     1741 tacccggcgg atttggaaag cggatctcaa tctcgatggg gcgccctcac cggacggccg
     1801 ctatctcgcg tttgacgatg agactggtaa cttggcggtg cgcgatctga ccacaggcga
     1861 ggtccggcgg ctgactaacg gcgcgggcgg ctggcgatac gcagcggctc cggtattctc
     1921 cccagacgcc gagcgggtag cgttcggctg gcgaaaccgg ccagatacgt gcgagctgcg
     1981 ggtcgcggca atggacagct cggagccgag agttctttat cgtgccgaag ggcaagaggt
     2041 tacgcccacg gactggtcgc tgaacggaag cagccttctg gtgttgagaa ggcgtgaacc
     2101 gtcccacagc gaaattgtta cggtggccgt cgccgatggg tctttgaacg tcctgaaaag
     2161 ctttgacgga gcctcacctg acgtgaggag gctacgcatt tcgcccgatg gccggttcgt
     2221 ggcctacgat ctgccggtgg acaaggcagg caatcgcgac atctttgtgc taacggtcgc
     2281 tggggggcag gtgagcccgc ttgttcaaca cacggcgcag gagcagttcc tcggctggac
     2341 gccggatggt aggtcggttc tgttcctcag cgaccggacc ggaaactggg gagcatggct
     2401 cattcccgtg gccaatggga agccgcaggc ggagcccact ctcgtgaaac gggacgcggg
     2461 ggccatggag ccattaggat ttaagctgag ggatctcaga tggctacagt ggtcgccccg
     2521 cggaaattcc ttgcttgttc gcggtagagg gggcttgtat acgatcgacg caaggaccga
     2581 ggcggtcagc cgggcaccgt ggtggccgcc ccgcgaatat ggtagttaca tcattgaccc
     2641 gtgctggtcc gcggacgaga aagcggtcta ttatgtagag ccgcgggtgt tagcgccagg
     2701 gggccaaacg cggctcctcc gggcggactt agtgactggc cgggaggttc tgattcattt
     2761 attcccgaag acggtgacgg gtggagctgt ccttctgtct cccaacggcg actggttcct
     2821 tttgtggctc ttacacggca ccgggtggag tccggctgtc atgccggcca acgggaccga
     2881 agtccgggat ctcccaggac cctggagcat gccacaggtc gtaccgcgca gcctgacatg
     2941 ggggccgcac tcgaaccagg tcgtttttat caagcatcct ggcacaccac cgaggatcgc
     3001 cacgtccgaa ctctggggcg tgccggcgga aagcggagag caacggagtc ttgggttctc
     3061 ggttgggaag gacgtgcagt tccccgtgtc cattcaccca gatgggaagc gattggcgtt
     3121 tacagcatgg gagcctgcga ccgaaatctg ggtgatggag aacttcctgc tgccctcctc
     3181 aaagtccggg aattcgagtg ctgaatgacc cgggaccgcg cagtctcgtt ccggtgggcg
     3241 agacagca
//
LOCUS       DATIFQ010000335         9204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2793909, whole genome shotgun sequence.
ACCESSION   DATIFQ010000335 DATIFQ010000000
VERSION     DATIFQ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793909"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VLE22_15405"
     CDS             complement(<1..780)
                     /locus_tag="VLE22_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB15843.1"
                     /translation="MKITRISTLVVNARMRNWVFVKVETDQPGLHGWGEATLEWKTKG
                     VVGAIEDLSVLLIGQDPRRVEHLWQVMHRQYFWRGGVTNYTAMSGIDQALWDIKGKEL
                     GRPVCELLGGPVRETLRFYDHLGGGSLEGMYKTGEPEQFAERMRESIEKGFTAVKAMP
                     IPVSEPIESASTLKHAARCVEAMRRAGGDSIDIMLDLHARTTPAAAIQFGRLLVDYNL
                     YWYEEPCWPEHVEGLVEVARALPFPIATGERLVGRWEFRELL"
     gene            complement(777..2180)
                     /locus_tag="VLE22_15410"
     CDS             complement(777..2180)
                     /locus_tag="VLE22_15410"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HSB15844.1"
                     /translation="METDQLLAGTGRVDITPAPGTPQGGWGAQTHQRGVAADMPFHAT
                     ALALAQGNEQVVIVDADAIGFDAEWTGRIVSAVSGLTSLPPERIRFSCTHTHSGPNTF
                     RLANISEGLDMAVSYLESLPHRIAGAAWQACQNLEPVRVAAASGECGISVNRRVRLPD
                     GRVIVGQNLEGEPDRTLRVVRFDRLDEEPVAIIMHYACHGTIMGWQCRHFTPDFPGPA
                     RQVVEQQMGCRSLFLQGAAADQGPRVGFTGDIEVYHRLGRELGLEAAKIATEIDTLTR
                     RQRLAGVMPSGANIALYEYERQEPEVPVLRVTSRTIRLPLKAFALPQELEAEVGRLWA
                     EAERCRGAGDVEGLRLANALATQAGWRLGNAQRYFGKQATEWPMQAIRVGPVAFLSIA
                     GEPFSAIGRRIASQSPFRHTLFSGYSNGGFGYIPERQDYDAGGYEIEATPFSADAADV
                     VVSEGVQLLWELAEEKL"
     gene            complement(2200..3810)
                     /locus_tag="VLE22_15415"
     CDS             complement(2200..3810)
                     /locus_tag="VLE22_15415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HSB15845.1"
                     /translation="MSHLDLGVFATYLVFNICLGLWVARRKTSTARDYFLAGERLPWY
                     AIGGSIIAANISTEHFIGMVGAAYAVGFVVAQWEWGTWLPFSALIWIFLPYYLRGGVY
                     TMPEFLERRYNKACRYLFAVASLALWIIAQMAVVMLAGAKAMEGMYGFDPPATIIGLA
                     VLAGSYTIYGGLISVAWTDFLQFVVLMIGGLIVAVKGVMKAGGVSFLMHAYPEKFRII
                     YPATDPNYPWFGIFALFISIGIWYNCTNQFIVQRCLGARSEWDARIGVVFAGFMKILL
                     PFLVVLPGIIAFHLFPGLTDPDQSYPRLVRELIPAGLGGVVMAGIASALLSHLSSVLN
                     SSSTVFTMDLYRPLLGKNREDAHFVRVGRWSAFVILGLSMGLAMWLTRGQHSVFLLIQ
                     NVGAWVAAPISVVFLAGVLWRRATSAAATFVLIFGFPYTALVEYVLFKHVAWLRPFDN
                     WLNRTFVVWATCLVAMAVVSWMTKAPEPERVKDIIWSRRCLALPPHERERNRGLRSLL
                     FWWALFIGLMAILYAYMIWFQFRDPGQG"
     gene            complement(3826..5142)
                     /gene="purB"
                     /locus_tag="VLE22_15420"
     CDS             complement(3826..5142)
                     /gene="purB"
                     /locus_tag="VLE22_15420"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HSB15846.1"
                     /translation="MIPRYTRPEMGRIWSDENKFRAWLEVELAAAEVLAERGEVPAEA
                     ARLLREHARFDVARILEIEREVKHDVIAFTTAVSESMAASGHAEASRWLHYGLTSNDV
                     VDTAQALQVSQSSDLLLDGVDKLRAALKKRALEHKHTVQIGRTHGVHAEPITFGLKLA
                     LWYDEADRQYRRLSAAATEMRVGKLSGAVGNCAHLDPDAEEAICAKLGLKAAPISSQV
                     ISRDRHANYIATLGLITATLEKIALEVRHLQRTEVREAEEPFTAGQKGSSAMPHKRNP
                     VTCEQICGLARVVRGNAQAAFENIALWHERDISHSSVERVILADSAILTDYLLAKTTW
                     LVANMRVYPERMRRNIELTRGLVFSGQLLLDLAAAGMLREKAYKVVQGHAMRAWEEEG
                     DFRAAINGDPEVRAVLSAEKIAESFSLDRQLRNVDGIFDRVFSERK"
     gene            complement(5147..5902)
                     /locus_tag="VLE22_15425"
     CDS             complement(5147..5902)
                     /locus_tag="VLE22_15425"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB15847.1"
                     /translation="MLASSSSGNCTFVSAGRTRILVDAGLSGRETFARLAAIGESPEN
                     LDAILVSHEHSDHVAGLPRLVKKLDIPVFLTHLTAPAIEWNDCEAHVEAFQAGRRISI
                     GDLEIDTFTVPHDAVDPVGFSFLSEGIKVSLVTDLGYIPDAIKYHLLGTNLMILESNH
                     DLEMLKVGPYPWSVKQRVMGRKGHLSNEVVSDYIRGDLDSTTSVLVLGHLSEHNNHPE
                     LVRLIARQALEGRELFTKLVIAEPRRQTEIFQF"
     gene            complement(5969..6406)
                     /locus_tag="VLE22_15430"
     CDS             complement(5969..6406)
                     /locus_tag="VLE22_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15848.1"
                     /translation="MTTKWKLILCAVALLAVFLLGFIPQFREARQRASELDAARKELA
                     TLRIESEASQLRDLAALMYMEVNRKNFGVASDHARAFFEMAGSLAGRTGEPALQEKLR
                     NLLALRDQVTAGLASADAGVGALVQELLLRAQEARPEKPRPEA"
     gene            complement(6437..7918)
                     /locus_tag="VLE22_15435"
     CDS             complement(6437..7918)
                     /locus_tag="VLE22_15435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15849.1"
                     /translation="MGRFVLISSLLVLAVVPEAQAQVCRLSVAGLNRARRAMGAIHAE
                     CPAIFTPHTPPFGNWGVTSNFGIKKDGHQFDGWCHDARVCNNLGICRTDCTNGWFEWN
                     SCTDDSLYRPPNCTLYNAADCTEQASTMGVNVHGTKVIDLAAPCPTDADGDGIAEHGG
                     CSAVSGYSPTTNFMSIYELDPGTSDELIQTLYFPETPVSLQCGVFGCPPAGSGWVAPV
                     AYDSPSSPAKIHAEVAAVVNWAAFLDENDRCAAATSAATAVSAASYTGPELSAGSIAT
                     VFSGGFSTTSEAATTLPLPFSLAGVSAIVTDSLGASRLAPLFFVSPSQVNLLVPPETA
                     GGGAAITLARGSKIRWKSAVTIAPVAPGLFSANSDGRGVAAAVTLLVRSDGSRTSQLV
                     FECPYSAGSCVPLPIDLAAGNDRAFLLLFGTGIRGRQALEDVRVRIGGIEAKVLYAGP
                     QGSFVGLDQVNVEIPRVLAGKGEVSVMLAVGAETANPVTVAFR"
     gene            complement(7945..8796)
                     /gene="hemQ"
                     /locus_tag="VLE22_15440"
     CDS             complement(7945..8796)
                     /gene="hemQ"
                     /locus_tag="VLE22_15440"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: protein motif:HMM:NF008913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HSB15850.1"
                     /translation="MASDTVETRMEEAPQVPLTLEGASVLHQMFRVRWSAWRALPEGR
                     RSEVVAEAAAAMEGMEGTALYSMLGHKGDLLLLHFRKSFEELNQAQLRVAGLALSDYL
                     EPATSYLSVVELGLYESSAKTYRSLLEKGVEPHSDDWNRQIEEMLAIQRKAMAPRLWP
                     EIPPNRYICFYPMDRKRAESKNWYQVPMQDRQRMMHEHGMIGRRYAGEVRQIISGSIG
                     FDDWEWGVDLFADDPLVFKKLIYEMRFDEVSAVYALFGAFFVGLRFAPRDLGAFLTGT
                     APGWAAK"
     gene            complement(8825..>9204)
                     /locus_tag="VLE22_15445"
     CDS             complement(8825..>9204)
                     /locus_tag="VLE22_15445"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSB15851.1"
                     /translation="RPLVDTSVWVAHVRATERNMVKLLETDRVLIHPIIIGELACGNL
                     RNRVEILENLGQLPSSVAADQGEVLELIEACHLCGRGIGWIDAHLLASTLITGCRLWT
                     LDRALGRVALELGVGWPQPKGGM"
BASE COUNT         1719 a         3155 c         2643 g         1687 t
ORIGIN      
        1 cagtaactct cgaaactccc aacgcccgac cagccgctca cccgtcgcga tcgggaaagg
       61 aagggcgcgc gctacttcga ccagcccctc cacatgttcc ggccagcaag gctcctcgta
      121 ccagtagagg ttgtagtcga cgagtaggcg gccgaactgg atcgccgccg ccggcgtggt
      181 gcgggcgtgc aggtccagca tgatatcgat gctgtcgccc ccggcccggc gcattgcttc
      241 gacacaccgg gccgcgtgct tgagcgtgga cgcgctctcg atcggttccg acaccggaat
      301 gggcatcgcc ttcactgccg tgaagccctt ctcgatactc tcgcgcatcc gctcagcgaa
      361 ctgctccggt tcgcccgtct tgtacatccc ctcaagcgat ccgcctccca ggtgatcata
      421 gaagcggagg gtctcccgga ccgggccgcc cagcagttcg caaaccggcc ggcccaactc
      481 cttgcccttg atgtcccaca acgcctggtc gatgccgctc atcgccgtat agttggtgac
      541 acctccgcgc cagaaatact gccggtgcat cacctgccac aaatgctcca cccggcgcgg
      601 gtcctgcccg atgaggagca ccgacaggtc ctcgatcgct ccaaccacgc ccttcgtctt
      661 ccactcgagc gtcgcctctc cccagccatg gagccccggt tgatcggtct ccactttgac
      721 gaagacccag ttgcgcattc gggcgtttac taccagggtc gaaattctcg tgatcttcat
      781 aacttctcct cggccagttc ccacagcagt tgcacacctt cgctcacgac gacatcggcc
      841 gcatccgcgg agaacggcgt cgcctcgatc tcgtagccgc ccgcgtcgta gtcctgtcgc
      901 tccgggatgt agccgaaccc gccgttggag tagccggaga acagcgtgtg gcgaaacggg
      961 gattgcgagg cgatccggcg tccgatcgcg gaaaatggct cgccggcgat cgacaggaac
     1021 gcgaccggtc cgactcggat cgcctgcatc ggccactcgg tcgcctgctt tccgaagtac
     1081 cgttgcgcgt tgccgagccg ccaccccgcc tgggttgcca gagcgttggc cagcctgaga
     1141 ccttcgacgt cgcccgcgcc ccggcagcgc tccgcttccg cccacaggcg ccccacctcg
     1201 gcctccagtt cctgcggaag cgcaaaagcc ttcaagggca gccggatcgt tcgagacgtt
     1261 acgcgcagca ccggaacctc gggctcctgc cgctcatact cgtacagcgc gatattcgcc
     1321 cccgacggca tgacgccggc aagtcgctgg cgccgcgtca gcgtgtcgat ctcggtcgcg
     1381 atcttcgcgg cctcgagccc cagctcgcgg cccagacggt ggtatacctc gatatcgccg
     1441 gtgaacccca cgcgcggacc ttgatctgcc gccgccccct gcaggaacag cgagcggcaa
     1501 cccatctgct gctcgacgac ctgccgggcg ggtcctggga agtccggtgt gaaatgacgg
     1561 cactgccagc ccatgatggt gccgtggcag gcgtagtgca tgatgatggc gacgggctcc
     1621 tcgtccaagc ggtcgaatcg gaccacgcgc aacgttcggt ccggctcccc ttcaaggttc
     1681 tgcccgacga tcacacgccc gtccggcagc cggacccgcc ggtttacgct gatgccgcac
     1741 tcgcccgaag cagccgctac acgtaccggt tcaagattct ggcacgcctg ccaggctgct
     1801 ccggcaatgc ggtgcggtaa gctctccagg tagcttacgg ccatgtcgag cccttcgctg
     1861 atgtttgcta ggcggaaagt gttgggtccg gaatgcgtgt gcgtgcagga aaaccggata
     1921 cgctcaggcg gcaggctcgt tagtccggaa acagcactga cgatcctccc cgtccattcc
     1981 gcgtcgaagc cgatcgcgtc cgcatcgacg atcaccacct gttcgttgcc ctgcgcaagc
     2041 gcaagtgcag tggcgtgaaa cggcatgtcg gcggcgacgc cacgctggtg cgtctgtgcg
     2101 ccccatcccc cttgcggcgt gcccggcgcc ggcgtgatat ccacccggcc cgtgccggcc
     2161 agaagttgat ctgtctccat ccccgcgaat cctcctcgct catccctggc ccgggtcccg
     2221 gaactgaaac caaatcatgt acgcgtacag gatcgccatg agcccgatga acagggccca
     2281 ccagaacaag aggctccgca gtccgcggtt ccgttccctt tcgtgcggcg gcagggcaag
     2341 gcagcgcctc gaccagatga tgtctttgac gcgctccggc tccggcgcct tcgtcatcca
     2401 ggaaacgacc gccatcgcga ccaggcaggt cgcccagacc acgaacgtcc ggttcagcca
     2461 attgtcgaac ggccgcagcc atgccacgtg cttgaacagg acgtactcaa ccagcgcagt
     2521 gtaaggaaag ccgaatatca gaacgaacgt cgccgccgcg gaagtcgccc gcctccaaag
     2581 tacaccggcg aggaaaacga cggaaatcgg cgccgccacc cacgctccca cattctggat
     2641 cagcagaaac acgctgtgct gcccccgagt gagccacata gccaagccca tcgacagccc
     2701 gaggatgaca aacgcgctcc agcggcccac gcggacgaag tgcgcgtctt cgcggttctt
     2761 tccaagcagc gggcgataga gatccatcgt gaatactgtg ctcgacgagt tgagcacgga
     2821 actgagatgg gagagcagtg cgctggcgat gccggccatc accaccccac cgagtcctgc
     2881 cgggatgagt tcgcgcacca gccgcggata ggactggtcg gggtcggtga gtccagggaa
     2941 gagatggaac gcgatgatgc ccggcagcac caccagaaac ggcaggagga tcttcatgaa
     3001 gcccgcaaat actacgccga ttcgcgcgtc ccattcgctg cgcgcgccaa ggcagcgctg
     3061 gacgatgaat tggttggtgc agttgtacca gatgccgatc gaaatgaaga gcgcgaaaat
     3121 gccgaaccac gggtagttcg gatcggttgc agggtagatg atcctgaact tctccgggta
     3181 cgcgtgcatc aggaacgaga cgccgcccgc cttcataacg cctttgacgg ctacgatcaa
     3241 accgccgatc atcagcacga cgaactggag gaagtccgtc caggcgaccg agatcagtcc
     3301 tccgtagatg gtgtagctgc ccgcgagaac ggcaagcccg atgatcgtgg cgggcgggtc
     3361 gaatccgtac atgccctcca tcgccttggc gccggccagc atcaccaccg ccatctgcgc
     3421 gatgatccac agcgccagcg acgcaaccgc gaatagatag cggcaggctt tgttgtagcg
     3481 ccgctcgaga aactctggca ttgtgtagac gccgccgcgc aggtagtagg gaaggaaaat
     3541 ccagatcagc gcgctgaagg gcaaccaggt cccccactcc cactgcgcga cgacaaaacc
     3601 caccgcgtag gcggccccca ccatgccgat gaaatgttcg gtgctgatgt tcgcggcgat
     3661 gatcgagccg ccgatagcgt accaggggag tcgctcgcct gcgaggaaat agtcccgggc
     3721 agtcgaggtc ttccgccgcg ccacccataa ccccaggcag atattgaaaa cgaggtacgt
     3781 ggcgaatacc ccgagatcaa ggtgggacat gcggcagcct ttgccttact tccgctccga
     3841 gaagactcta tcgaaaatcc cgtccacgtt tcgcaattgc cggtccagcg agaagctctc
     3901 tgcgattttc tccgcgctca gcacggcccg cacctcggga tcgccgttga tagccgcccg
     3961 gaagtctccc tcctcctccc acgctctcat cgcatgtccc tgaacaacct tgtacgcctt
     4021 ctcccggagc atgcccgccg ccgccagatc cagcagaagc tgcccggaga acacgagccc
     4081 tcgcgtcagc tcgatgttgc gccgcatgcg ctccggatac acgcgcatgt tcgcgaccaa
     4141 ccacgtcgtc ttcgccagta gatagtcggt caggatcgcg gaatcagcca gaattacccg
     4201 ctccaccgac gaatgcgaga tgtcgcgctc gtgccacagc gcgatgttct cgaatgccgc
     4261 ctgcgcgttc ccccgcacta cgcgcgcgag cccgcagatc tgctcgcaag tcactgggtt
     4321 gcgcttgtga ggcatcgccg agctgccctt ctgccccgcg gtgaacggtt cctccgcctc
     4381 gcggacttca gttcgctgaa ggtgccggac ttcgagcgcg atcttctcga gcgtcgccgt
     4441 gatcagtccg agcgtggcga tgtagttcgc gtgtcggtcc cgggaaatca cctgcgacga
     4501 aatcggcgct gccttcagcc ccagcttcgc gcagatggcc tcttcggcgt ccgggtccag
     4561 gtgagcgcaa ttgccgacgg cgccggaaag cttgcccacg cgcatctcgg tcgcggctgc
     4621 cgacagcctc cggtattgcc gatccgcctc gtcataccac agcgcaagtt tcagaccgaa
     4681 cgtgatcggc tcggcgtgga cgccgtgcgt gcggccaatc tgcaccgtgt gcttgtgctc
     4741 gagcgcccgc tttttcaacg ccgcacgcag cttgtcgacg ccgtccagca gaaggtccga
     4801 cgactggctc acctgcagcg cctgcgccgt gtccaccacg tcgttcgacg tcaggccgta
     4861 gtgcagccag cgcgaagcct cggcgtggcc ggatgccgcc atgctctccg atacggcggt
     4921 tgtgaatgcg atgacgtcgt gttttacctc gcgctcgatc tcgaggatcc gcgccacgtc
     4981 aaaccgggcg tgttcccgta gcaaccgcgc ggcctcggca gggacctcgc cccgttcggc
     5041 cagcacctca gccgccgcca gctccacctc cagccaggca cggaacttgt tttcatcgct
     5101 ccagatgcgg cccatttcgg gtcgggtata tcgcggaatc ataggattag aattgaaaga
     5161 tttctgtttg tcgccgcggc tcggcgatta ccagcttcgt gaacagctcc cggccctcca
     5221 gcgcctgcct cgcgatcagc cggacgagtt cggggtggtt gttgtgctcg ctcaaatggc
     5281 cgagtaccaa taccgacgtt gtgctgtcca gatctccccg gatgtaatcc gagaccacct
     5341 cgttcgacag gtgccctttc ctccccatca cgcgctgttt caccgaccag gggtacggtc
     5401 cgaccttcag catctcgaga tcatggttcg attccaggat catcagattg gttcccagca
     5461 agtgatactt gatcgcgtca gggatgtagc caagatcggt cacgaggctg accttgatcc
     5521 cttccgagag gaaggagaac ccaaccggat ccaccgcgtc gtggggaacg gtgaacgtgt
     5581 cgatctcaag atctccgatc gaaatgcggc gccctgcctg gaacgcttcc acgtgcgcct
     5641 cgcagtcgtt ccattcaatc gccggcgcgg tgagatgcgt cagaaagacg gggatatcca
     5701 gctttttcac cagccggggg agtccggcga catgatcgct gtgttcgtgg cttaccagga
     5761 ttgcgtcaag gttctccgga gattcaccga tagccgccag ccgcgcgaac gtctcacgtc
     5821 cgctcagccc tgcatccacg aggatacgtg tccgcccggc ggacacgaac gtgcaattcc
     5881 cggagctgct gcttgcgagc acacaaacct tgacgataat cgcctcccgg aacttctctt
     5941 gtagcaggaa accggcggca caacaacatc atgcttcagg tcgcggtttc tcgggtcgtg
     6001 cctcctgcgc acgtagcaac agttcctgaa cgagcgctcc cactccagcg tcggcggaag
     6061 ccagccccgc ggtcacctgg tcccgcaacg ccagcaggtt cctcagcttc tcctgtaacg
     6121 ctggctcgcc cgtacgtccg gcaaggctgc ctgccatctc gaagaacgcc cgtgcgtggt
     6181 cactcgccac gccgaagttc ttcctgttca cctccatata catcagcgcc gccaggtccc
     6241 gtagttgcga cgcttcgctc tcaatccgga gcgtcgccag ttccttccgg gcagcatcga
     6301 gctcgcttgc ccgctgccgc gcctcgcgga attgcggaat gaaaccaagg aggaaaacag
     6361 ccagcagtgc gaccgcacag aggatgagct tccacttcgt tgtcatgtgg ttcccctagt
     6421 aagcctctct ccggcttcaa cgaaatgcaa ctgtcaccgg gtttgcagtc tccgcgccca
     6481 ccgcaagcat caccgagacc tcgccctttc cagccaatac ccgtgggatt tctacattca
     6541 cctggtccag tcctacaaag gaaccctgtg ggcctgcata gagcaccttc gcctcgatac
     6601 cgccgattcg cacgcgcaca tcctcaagcg cttggcgtcc acggatcccc gtcccgaaca
     6661 gtagcaggaa ggcgcggtca tttcctgcgg ccaggtctat ggggagcggc acgcaactgc
     6721 cggcgctgta cgggcactcg aacacaagct gcgaagtccg ggagccgtca gatctaacca
     6781 gcagcgttac cgccgccgcg actccccggc cgtcactgtt tgccgagaag aggccgggcg
     6841 cgaccggtgc aatcgttaca gcgctcttcc agcggatctt cgacccgcgc gctaaggtga
     6901 tggccgcccc gccgcccgcc gtctcagggg gtacgaggag gttgacctgg gatggcgaga
     6961 cgaaaaagag cggcgccagt cgggaggctc ccagactatc ggtgacgatc gccgatacgc
     7021 ctgcgagtga gaagggaagt ggcagcgtcg tcgctgcctc gctcgtcgtg gaaaaaccac
     7081 cactgaatac cgtcgcgatc gatcctgccg agagttccgg tccagtgtag cttgcggcgg
     7141 aaaccgcggt cgccgccgat gttgccgccg cgcatcgatc gttctcatcg agaaacgccg
     7201 cccagttcac tactgccgcc acctcggcgt ggatcttcgc cggcgagctc gggctgtcgt
     7261 acgccaccgg cgccacccaa ccactcccgg ctggcgggca gccgaagacg ccgcactgaa
     7321 gagataccgg ggtctccgga aagtacaacg tctggatcag ctcgtcactc gtgcccgggt
     7381 cgagttcgta aatcgacatg aagttggttg tgggcgagta cccggatacc gccgagcatc
     7441 cgccatgctc agcgatcccg tcaccgtcgg catccgtcgg gcagggcgca gcgaggtcga
     7501 tcacctttgt tccatggacg ttgacgccca ttgtgctggc ctgttctgtg cagtctgccg
     7561 cgttatagag ggtgcaattg ggcggccggt acaaggagtc atccgtgcaa ctgttccact
     7621 cgaaccagcc gttggtacag tccgttcggc agatgcccag gttgttgcag acccgcgcgt
     7681 cgtggcacca gccgtcgaac tggtgcccgt ccttctttat cccgaagttg gatgtgacgc
     7741 cccagttccc gaacggtggc gtgtggggag tgaaaatcgc cgggcactct gcgtgaatcg
     7801 ctcccattgc acgccgcgcc cgattcagac cggccactga aagccggcag acctgggcct
     7861 gcgcctccgg gactacggcc aaaaccagca ggctggagat tagcacgaac ctgcccaaac
     7921 gccgcccctc cgcatgcttc cctgttactt tgccgcccag ccaggtgccg ttccggtcag
     7981 gaacgcaccc agatccctcg gcgcgaaccg caagccgaca aagaaagccc cgaatagcgc
     8041 atagactgcg ctcacttcgt cgaagcgcat ctcgtaaatc agctttttga acaccagtgg
     8101 gtcgtcggcg aacaggtcca cgccccattc ccagtcgtcg aagccgatcg aaccggaaat
     8161 gatctgtctc acctctccgg catagcggcg gccgatcatg ccgtgctcgt gcatcatccg
     8221 ctgccggtcc tgcatcggca cctggtacca gttcttcgat tccgctcgtt tgcggtccat
     8281 cggatagaaa cagatgtagc ggtttggcgg aatctccggc cagagccgcg gagccatcgc
     8341 cttgcgctgg attgcgagca tttcctcgat ctgccggttc cagtcgtcac tatgaggttc
     8401 gacgcccttt tcgagcagtg agcggtaagt cttggcgctg gactcgtaca gtcccagctc
     8461 caccaccgac aggtacgagg tcgcgggctc gaggtagtct gacagagcca gtccggctac
     8521 acgaagctgt gcctggttca gttcctcaaa cgatttccgg aagtgcaaca gcagcaggtc
     8581 ccctttgtgt cccagcatgg aatagagagc cgtaccctcc attccctcca tcgctgccgc
     8641 cgcttccgcc acgacttcgc ttcgccgccc ctccggcagc gcccgccacg cggaccaccg
     8701 cacccggaac atctggtgca gcacgctcgc tccctccagc gtcagcggaa cctgcggcgc
     8761 ctcctccatc cgcgtctcaa ccgtgtcact ggccattgaa tttcgacgcc ctccttgttt
     8821 caatctacat gcctcccttc ggttgcggcc acccgacccc caattccaat gctacccggc
     8881 ccagcgcgcg gtcaagcgtc cacagccggc atcctgtgat cagcgtcgac gcgagcaagt
     8941 gcgcgtcgat ccagccgatt cctcttccac agaggtgaca cgcctcgatc agttccaaaa
     9001 cctcgccctg atcggcggca accgagctcg gaagctgccc cagattctcc agaatctcca
     9061 cgcgattccg cagattgccg cacgccagct ctccgatgat gatcggatgg attagaacgc
     9121 ggtcggtttc taacaacttc accatattgc gttccgtggc ccggacgtgg gccacccaca
     9181 ccgacgtgtc gacgagaggc ctcg
//
LOCUS       DATIFQ010000336        30878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1261919, whole genome shotgun sequence.
ACCESSION   DATIFQ010000336 DATIFQ010000000
VERSION     DATIFQ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 30878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30878
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261919"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2405)
                     /locus_tag="VLE22_15450"
     CDS             complement(<1..2405)
                     /locus_tag="VLE22_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15852.1"
                     /translation="MNNYRSITVVVLLALVACTGVWAQSTAGTVRGMVKDPSGAAVPS
                     AAVTLLNVETRSVRQAQSDENGLYSFPNTFPGKYQLLVEAQGFKKWSGNLELQVQQLA
                     VIDPGMEIGDLAAAVEVRAVTPVIAAESSSIASVTESERIRDMPLNGMDITQLFQLTP
                     GVEAGSYSPHVAGMNPGAAEILQDGGSIVDRMRGGLPRVNLALDTIQEFGVDTNSTAQ
                     FSHPATITMVTKSGTNEFHGSVFEKFRNNAAGLRARARQDGNNPDPYKRNEFGASAGG
                     PVRIPGLYNGKDRTFWFFSFQGMRLREYSSTQDIVPTEAMWKGDFSNLDDGAGHPYTI
                     YDPYSTTASGTRTPFTGNMIPSTLGGNKLVSYLSANTPRPTTPANPLAENNYFATSAD
                     PASYNSYNFKVNQYIGGKDTLAGRFTIADNNWSGKFGGGPVSPEGQYNSIGEVDKVYN
                     GTITHVHTFSPLLLNELVLSGQRSRSTRGGAREDVKWTNQYLGLSNPLDEYGWPTITG
                     GNGFLWDSENKLPERLNKLMAEDNLTWLHGKHEFKAGFRYANERNNTRGARQGQGRYK
                     YQGDWTALWDPEGQHPMDFTGLGMADMFLGYGSYYRCNYSRPYYYLRQGELGFYAQDS
                     WRVTPRLTLNYGLRWDYWSPYNESANRIFALDMSKWQTATQLISPAGHPAESLGVPPS
                     VLASYAANDLSWTTADKAGYPSNLMNGDKEDFAPRIGAAYKLDRKTVVRGSYGMYYWT
                     VPNSQMLSSQLGSVPLALEYTMMMDWWEVEPGKRVDWYDVFHTPLPGIRVGDPNMIDI
                     TNL"
     gene            2756..3820
                     /locus_tag="VLE22_15455"
     CDS             2756..3820
                     /locus_tag="VLE22_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSB15853.1"
                     /translation="MSSQKKVTLRDVARLAKVSPSTVSRVVTRSAWVSPEIEARVRSV
                     ARKHGLDLESKIKARVIAFVLSNRDMLHPFHSRVLVGAEAYCTAHGWDILFLTFRYPA
                     GIPASDVRLPDVLRRRDLVRAVILAGANSPNLLGALKDQGLPFAVLGNNVEGDWRAED
                     YDVVWSDDVAGSVELTRYFLSLGHRDIWFVGNRRLPWSARSCQGYSQVMQETGLAPRV
                     SSINSLDDEDLGYLGTKSILSRNEPISAVIASSDQAAAGVYRALRDSQKRVADDISVA
                     GFNDTEATVLQPPLTSVREFGQEIGRHLAEQVINRIHQPDLPPQNVIVPTELIKRASC
                     REFSPDRRADTGRLAAPAVS"
     gene            3920..4276
                     /locus_tag="VLE22_15460"
     CDS             3920..4276
                     /locus_tag="VLE22_15460"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB15854.1"
                     /translation="MEPKKLMAVDPKTLPWEEVFNEPTGKYLYRRSLVTDPDTGMEIK
                     IVRYPAGFTNPLHTHHCAHGMYVLEGTLKTHEGSYGPGSFLWFPEGMTMEHGATAEQD
                     VVILFITNKPFDIQYL"
     gene            4806..4994
                     /locus_tag="VLE22_15465"
     CDS             4806..4994
                     /locus_tag="VLE22_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15855.1"
                     /translation="MSRALVALAERGVQAEAEAQEQLKTAYRRFLNENEPARKNEAGK
                     ELIRAIFGKDAIGEDQVL"
     gene            5129..6133
                     /locus_tag="VLE22_15470"
     CDS             5129..6133
                     /locus_tag="VLE22_15470"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HSB15856.1"
                     /translation="MNPVVSRGFCFAILIVLSAAGLSAAEFLPLAPGNNWTYRDAVTG
                     SNFTVEIGLVQMYANNHVYHMLRGYTPMPVWVRINEYGNAVFWDEAQRSDVMLTAFET
                     GFGGWFEAGARECPEQGQAQEKRGVHHGPAGEWNVLEIQYRTLECADTGDLLEQFAEN
                     IGMVRRVVNTIAGPRTFDLVHARLGALSISAGNAGGFSVTATLSPEPGYWEATLRVDQ
                     RSGDSLKVRFPTSQEYDLRLRDSGGNVVWTWSAGKVFDPPEHELHLLDGWKASVKVPI
                     PLVPPQGPQTYTLEAWLTVADGEPRFAAATTVKAPGTTGVVALRQGRIKIQPPGRRKN
                     "
     gene            6342..7616
                     /locus_tag="VLE22_15475"
     CDS             6342..7616
                     /locus_tag="VLE22_15475"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15857.1"
                     /translation="MKPHLVRGAGKEKLKVGLVGCGGRGTSAVFELMAATDNVELTAM
                     ADVFEDKLESSLERLRSDRNLKRFAGQTVIRDGKPYQLTVEDMAKKLQAGIKVAPDRH
                     FVGQDAFKKLLATDIDAVLLITPPGHRPLHFEAAIEARKHVFLEKPVATDPVGTRRVI
                     AAGRKAKELGLTVVSGTEMRYTPQYMETVEQIQAGAIGDITAAYAYWYGTAIFHASER
                     KPEWNEIDWQHRNWYSFVWICGDQLVEQQVHRIDLVDWVMKSHPIKAIGNGGRAWRRN
                     DNPVHGDVFDIMDVEFTYPNGVKLISMSRHYPRYDHLPMRNGAEVVGAKGRSNCMDMA
                     RTKTEIIGGMQEQVRLINSIRGDGPYINNTEEVAESTMVCIMGREAAYSGQEVTWEQV
                     MNSQLDLYPKDLSPGAEIPVPKPRVPGEYRFV"
     gene            complement(7677..9692)
                     /locus_tag="VLE22_15480"
     CDS             complement(7677..9692)
                     /locus_tag="VLE22_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSB15858.1"
                     /translation="MSLSLRSGAGLTSVRLVHKPVFAWGRWTALLWLALCLLPLRSQA
                     QTGIAVPELESFDQWMPDYLNRWNIRGASLAVAKDGRLVFARGYGLADVEAREPVEPE
                     SLFRIASVSKTLTAAGILKLVDEGKLDLEAKAFRLLDHLTPPPNTHPDPRLFEITVRQ
                     LLHHTGGFVKDKNGVANSNDPMRPPMTDMASDALGVPRPASPETIIRFMMGRPLDFDP
                     GTDYAYSSFGYCVLGRIIEKVSGQPYADYVQQAVLAPSGISRMRLGRTRLRDRAPGEV
                     RYYSDPMPLYTSVYGEGLVPFPYGAFSMEGIDASGGWIASAMDLVRFASALDGLHKPA
                     VLKPEIVRLMLSPPSPPVWSGQDTYYGLGWHVRTAAPEAYWWHFGGTPYSSSAFLGRL
                     PGGICLAVVLNGHVVAGDYWREMQSALTSAAENIAAWPAHDLSPRYYAEDAPSFSRTG
                     VVNAASFAPGPVAPGEIVTIFGERLGPAALTLAGFDSTGLLEKTAGGTRVLFDGVAAP
                     VVHSFATQVSAIVPYAVAAASTTEVIVEYQGVASRPVTLDVAPSAPGLFMVAVNSDQS
                     LNSELRPAARGEDIVLYATGEGQTNPPGVDGKLALESWPKPVLTVEASIGGQSAKVVY
                     AGAAPGMVAGLMQVNLRIPLDVQPGNAVPVVLLVGSRSSAAFPIAVR"
     gene            complement(9673..10812)
                     /locus_tag="VLE22_15485"
     CDS             complement(9673..10812)
                     /locus_tag="VLE22_15485"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB15859.1"
                     /translation="MTHRERVLAALEHREPDRVPMDLGSARFTHIVKGAYDRLCAHLG
                     FGNAGPLIDRMQQLYELDERVLQYLDVDVRPVALGSPDRGGDVELEDGGYRDEWGVER
                     RRPPGCPYYDMTFSPLAGRITAADIARHPFPDPTDPGRFRGLRERALRLREQTDCAIC
                     LNARFHLVHQTQYLRGFHDWYLDLGEDHDLFHCLMNAVLENLIEMNTRALREVGDLID
                     VVGFGDDIGLQDRPVCSLPMYRKMIRPYQERIVATMRAHTNAKILYHTCGSVSRYIGD
                     FIDIGINALNPVQVSAKNMEPERLKTEFGDRMAFWGGIDSQHILPHGTPDDVRAAVRR
                     TFDVMGKGGGYVLASVHNIQPDVSPENILALFETGKECVYEPVAA"
     gene            complement(10846..13464)
                     /locus_tag="VLE22_15490"
     CDS             complement(10846..13464)
                     /locus_tag="VLE22_15490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSB15860.1"
                     /translation="MTEHMALAPTLRLHAAEMPDAPAILAPGRPPLTFAGLSAHVESL
                     ARALAGGGFAGDNVVAAVLPDDAEGLYAFLGISSVACFAPLNPAFRAEEFESCLEDLR
                     PSALLVPAGTRSPAVGAAEALGIAILESQAAPEHGAGVFRLTGLNQAIAASDIPDPSA
                     VALSLLTSATTGRAKCVSITHANIEAMALASAQAMMLSRTDRFLSMMPLFHLQGLLSA
                     FAQLMVGGSVVCAAPFDADRFPVWIDELQPTWYTAGPALHRTILGLCREKPELAMRSR
                     LRFVRSIGAALPAALLRELEETLRVPVLEGYGLTEVGAVTSTPLVPYASKAGSAGIRI
                     VPGVEIMDEAGNLLPAGHVGEIVVRGPNVMRGYRNNPEATRAAFRNGWFRTGDLGRFD
                     DEGFLYVTGRLKEFVNRGGEKIQLQEIDRALMAHPVVADAAAFAIPHPQLGEDLAAAV
                     VLRKGERFDESALRDFLAARLAVFKVPREFMIVEGLPRGASGKVQRVRLAAEFADLQR
                     ARATAAAAVAPRTDCERRLAEIWAAVLGIPTPGIDGDFFELGGHSLASTRILARIEEA
                     FGIRLRHDALLAAPTARRLAVLLQSADAQRERIVAIQPEGSKPAFFMVRPLPIFRPLA
                     GRLGTERPFLGVVMPRLEQLAGGSDVPSVACQLVSVVRQQQPSGPYHLGGWCADGLLA
                     FEMAQQLTAAGERVALLALFDTPNPALLRRRSLRLTAKRLLGMLRWRVRFQLASVCQL
                     AMKEVPAHVWERMVALGGYVVGGLRALLHPDERANDAFSGIHHPSGIRLRISNYQHQT
                     YPGRLTLFRAAGRPTEDPSYGWDGAARDGLTVHTVPGDHLTMFLEPNVDVLTRKLAAE
                     LERVDDVVPPSTTFRK"
     gene            complement(13461..15179)
                     /locus_tag="VLE22_15495"
     CDS             complement(13461..15179)
                     /locus_tag="VLE22_15495"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HSB15861.1"
                     /translation="MRRRAASYAGQQRLVVDYYRIRRRLAFPLPLRSAVLPSVPVPTI
                     SDYPWTVWLLWELEERVNSLGWAAEWFGNPEYVRLAARDLEALSEWPKFRQYEQPDLG
                     AGHAGRLLWSALTKWRWPDAELRKKLRAACVRHVEEVAPASDSHYRGIRTRQDLLSQP
                     DVPRWLHNIPLIGTVGAALAAAAAGHPARAALNQRIQAIMGAILDFRNTGYTEGVAYD
                     GYILDFVADWLEALPLNERKEILEHPHLKHFLEESYMLSAPGAMEQVAELGDVEPREM
                     PFHFSAQAKLASLQADPLRAWYLERWRSEWIRADALGALRASASLPRRVAPEAGALNA
                     HYALVLRTGWESEDLAAAVSCSNSPMGHLQLDNGTVVIGTRGRWILSDPGYQQYMQDI
                     EREFTIGPAAHNYPLINGIAQDRKMPRLMRLGKTGDVLSAAVELAGCYPPAAKVRSVV
                     RTVWVSKRNLVVVADHVEGESVETLSYHWHAHPEAALWPQDGAVLLHTPEAELWLASP
                     QVPLSDDAIRRLPGSRGQLTVVAGVAPAPPVVWWVFALGGAPPRVEVSDRGRQIAVLG
                     RQFEIQ"
     gene            15428..15673
                     /locus_tag="VLE22_15500"
     CDS             15428..15673
                     /locus_tag="VLE22_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15862.1"
                     /translation="MYSNILISILILLVAFQVIGGMLSTPKLIKAASVGIVLLVAAAL
                     IIWLLMGLSPIPPWISKFVVKIYRQAAAAAASLDKAK"
     gene            15776..16546
                     /locus_tag="VLE22_15505"
     CDS             15776..16546
                     /locus_tag="VLE22_15505"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSB15863.1"
                     /translation="MFEQAFVEGVGRTRRPWTVVVSFMGQVGVVGVAVVLPLVYTEVL
                     PRTQLVSLFFAPSPPPGRRAPQPVPAPKAGVKIPPREFDGRKLTEPGAVPEKVASIID
                     IEEPPLPYAGPGSEVGVVGGIDLPPEALSPAVRDALNSGAAARVAPPPPASKPPEEKI
                     VRLRVGGVVRPPVPVYEPRPVYPQIARQARVSGVVHLEALIGTDGTVRGLQLVSGHPL
                     LVQAAMEAVRTWRYTPTLLNGDPIEVVMLIDVSFTLSK"
     gene            16614..17582
                     /gene="selD"
                     /locus_tag="VLE22_15510"
     CDS             16614..17582
                     /gene="selD"
                     /locus_tag="VLE22_15510"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HSB15864.1"
                     /translation="MSPKVLDQVLARLPRVANENVLVGFDTSDDAGVYKLTPECALVQ
                     TVDFFTPIVDDPYNFGAIAAANSLSDVYAMGGKPVSALSIVAYPGKGDLDDLEQILKG
                     GADKIHEAGCVVIGGHSITDDEIKFGYAVTGVVHPDRVKTNGGARPGDALVFTKRIGT
                     GVISTALKRGIAREEDVRVSVESMLALNRSACEAMLEFDVHSCTDVSGFGLIGHAREM
                     ALAGGVTLELQAEAVRFLPGAVEYARAGAIPGGLKNNREFASCVVEKTRPISDEIENL
                     LYDPQTSGGLLIAMPDDDAARFITKCEYAYRIGLVLARQEKAIRLA"
     gene            17602..19164
                     /locus_tag="VLE22_15515"
     CDS             17602..19164
                     /locus_tag="VLE22_15515"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HSB15865.1"
                     /translation="MRILLVSLACFAVAGAVSAGTLSVKILDENGSPAPARVYLTDSS
                     GQLQVPAGMIVYKRTRNVSEEHFVPPSGAFKIDLRSGVYHLVVERGKEYLPAGENIRV
                     SGGAAEKTVRLRRWVRMTDRGWYSADMHIHRPLLDLGAIMEAEDLNFAVNITRWRVGD
                     REIAEDPELDAFLAKSDANGAMKSANERWFTVLNEELEPPESALLVSRMGKVRSTLEY
                     PMAAYGRALRQRGALVDSEKATSLELPAIAALGGCETVGLANNHLWRSGSFPGAWGAW
                     PDRMMRAYPADCSGFALAGFEMYYALLNAGLPLKLSAGSANGVHPVPPGWSRIYAHLD
                     GEFTVERWFEAVRAGRTFVTTGPMLLLTVNGLGPGQEPRGAKFPMKAKVNVTVLSPDP
                     VREVELVVNGDVRKLPLRGSGMTWSGASDLELTTTTWIAARWMSGGDKVCGVAHTAPV
                     LFRNGSQPVFVRRTEAEYLVKAVERLIDDVKAGRKGPGDAIPMIVTPTPVVREQTLGL
                     LEQARDFYRGKL"
     gene            19250..20362
                     /locus_tag="VLE22_15520"
     CDS             19250..20362
                     /locus_tag="VLE22_15520"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HSB15866.1"
                     /translation="MFAAFAGEPVSADQIRRRLPKDTPSGVAAILAATLNSDPASHNT
                     DWFGTLLMKGLLEWGRRYVPEAGPFARTWLEAHLNRDTVSPYSGHRSRPARAGGVPIT
                     TYAGQFGLCFPCYEIYTQSKDERARRVCIELADIILHEVARNRFGLVAHDDTAEFAIP
                     DTCYFAVSALILASALDRTGGAVYRRQAVFQLRTYTDTFLMPDTGLAKTILLKDGLGK
                     TYWTRATGWLLWSITSVLGHLPPEDPAFAGFVRDARKLADGISRVQDESGGLRLFLNE
                     PDSPLETTGTAMCAMGLHEAIRRRWLPASYTPVVERAWKYVTGNITPEGDIRRAYTGW
                     AVPAENHEVELDQRKMGWIPGFILSTAYELTAAGRT"
     gene            20577..21203
                     /locus_tag="VLE22_15525"
     CDS             20577..21203
                     /locus_tag="VLE22_15525"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HSB15867.1"
                     /translation="MRKVFAVVSLGLAVLPLLLHGQTTETQRFQALMSSTQENSAALV
                     GYQAMGSAVVEFFLKRDAQGALLSAVTDFRIEHTFGQAETVTNMHIHRGAVGSPGPVV
                     VPSLFGAAVDVTAGSGAFFRAAQTTDPVILAVIEEILANPGRFYVNVHSLTFPGGIMR
                     GQLERDPARAAARRDEALAAEIEGIKEYELLSGRILRLIGLRMGVIAP"
     gene            21741..22517
                     /locus_tag="VLE22_15530"
     CDS             21741..22517
                     /locus_tag="VLE22_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15868.1"
                     /translation="MTLVASTFPNVPDFTCDSWCYEIPVDFTGTAAISGGRIELRHRV
                     LRYPQVILVTTVTPEPGAVEFVVRPELDKGATGEVPAGLLLPNLCWQLIRAPAFASKP
                     EAYPEFAKRCFIFTEKGRTFLGKTTRRNLPNRPEYESMNNPVWAQMYTAVWSRFPSVL
                     PRWSVNFPTTVWSEANSPDRYTAPVIGTVSRDGKYLAALGNGSAETMLQAFHDCLHNN
                     PEWLPPAAPPAQQSWRLKIYVMENDPEALLKRVAKDFPRP"
     gene            complement(22594..25899)
                     /locus_tag="VLE22_15535"
     CDS             complement(22594..25899)
                     /locus_tag="VLE22_15535"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB15869.1"
                     /translation="MFQRKRCLLTLVALGLLIAGATWAQTTFGVIRGRVLDPTGAAIP
                     KVNVVVTNTGTNISRTVVTSDTGAYETGYLQPGTYSVTAEASGFRKFVSEGIVLSANA
                     IVLVDANLAVGELTSSVTVEAGAPLISTETATVTDVKTQEQYLKAPMNVRGNWDSFLF
                     NFMSLVPGVQPMGTGFSISFSGSRYTVNNFTVDGITTNSVLYGNIVGPANPSMDFIQE
                     VKVDVSGTSAEFGAPGYVGVITKGGGNEVHGSAFWYYNSAGFNARNPFANRVGFAVLN
                     DYGFTVTGPVFKNKTFFSGGLEGFNQHTAALFNLSLPTDRMRAGDFSVLKDSAGKLMS
                     VTIKNPLTGLPFENNKIPASLLNSAALKTQERFYPHVNFGDPDMVVSNFRDQAKQTLR
                     KEQIDIRIDHQFSSKNSFFGRFDAMRAPNGTWEGNLPTVGPRVQRRQTRNLILSDTHT
                     FSPTVINEFRYGLIRGYNPYSGPVDGPTVVKEMGLTNLPADLPQLQAIPIVNISNFQT
                     ISQIDYQRGAERVDQWQDNLSVIRGRHTLKMGGEVWRNYGQNFGQSPSRAYGSVSFTG
                     NYTGYSYADFMFGIPRSASRVSAGFVLLTSTNWDKFLFFQDDFKVNPRLTLNFGIRYE
                     LNPPYVEEKDRIFSFNPYTGKMVVPNEQAKAALFSGFVASNLVPIITAKEAGLPERTL
                     AYTDKDNLAPRFGFAYKLTSDNKTVLRGGFGFFYDTYTAALWRSMVGGPYNGSESSPI
                     NAVTNGQALWMWPDMFPRVLSQAGTASLSGQDPNTAVPYTEQWSVTLEREIGNMGLRA
                     AYVGSKTHQLLASRNLNQLVPSTTPYSASRRPYPQLSSAGWRENMLNAYYNGLTLSAE
                     RKYKAGLQYQVGYTWAKNLTDSHDDWEAGWGAQNAYDYRPEWADNAYTRRHRFVFNSI
                     WDLPVGKNQKFLREGVLSHIIGGWSISTFGVLQTGRYFSPAFSTYDPANTGTSGGRPD
                     RIGNGNLSDPTIAMWFDKNAFRVPGDMDGNGTPDVQVGRFGNSGVNVLRGPGTRSMNA
                     GFFKAFRLREKWRLQAEATFTNVFNHPNFGIPNSTINSGAGGLITQTQNQQYSAHEGS
                     GERTTRFGLRLDF"
     gene            26135..26413
                     /locus_tag="VLE22_15540"
     CDS             26135..26413
                     /locus_tag="VLE22_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15870.1"
                     /translation="MAISRKTVCITWVALGFGLVIAGGLTSLYVLPAIRQVSERIPLS
                     LLSVVAVLAGIFLIVRFALKLEDVGGPAQEIHIPGLDTDVRSTGQSAK"
     gene            26712..27146
                     /locus_tag="VLE22_15545"
     CDS             26712..27146
                     /locus_tag="VLE22_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15871.1"
                     /translation="MAKINASRVLLGGLLAGIVINLFEFVSNAVIFKDDIAAAMAALN
                     KTMDMSGGTMAIYVCWAFLIGIVAVWLYAAIRPRFGAGLVTAVKTGLVMWVLVAVQTT
                     ISFAPMGLFPRRLMAIGLVISLVELVLATVLGAWLYKEEAAT"
     gene            complement(27721..28908)
                     /locus_tag="VLE22_15550"
     CDS             complement(27721..28908)
                     /locus_tag="VLE22_15550"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSB15872.1"
                     /translation="MTLARRSETADIVRRKFWLDGGQSTPGGEKGVTGVRVEGGVCLR
                     RSKRHGRIASETVVSGWAVAACSVPVQATVAFRAIANGVPQQEITAQPTLPTPVYTSA
                     ANRFVGVALANVYSVPVTVRLTVYDSEGLVIGGPTDVSVASFGHTTFNIWQKFPHLRD
                     RDFDGVLKITGTPDLFVAWSLNGDASGTLSALPPGALAWPISHWDRIWYVFKQVEDGA
                     KQLGAITWPVQLKILYDKEVNAYARRGDEIGVTLGLSELISDSPSELAWALAHELGHT
                     YQQRTGKLDFVPTNREFDADIWGTMLTLAAGYDPYAGSGTLAKLAMATGRAALTTQFE
                     DQIAADAHKSFNTRIDTIYDNLVEACSVPSVQPSCSLYKLIVHPHLPPSAPLVREPRQ
                     ARE"
     gene            29300..29629
                     /locus_tag="VLE22_15555"
     CDS             29300..29629
                     /locus_tag="VLE22_15555"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB15873.1"
                     /translation="MISVRPARFPIVREVRYKVINSKGAVEIGTGETINISSTGVLFN
                     AQAPLRPGKRIELSISWPAQLDGKHGLKLVARGRIVRCEGTNVALEIENHEFRTVGRG
                     LGALAGI"
     gene            30274..30531
                     /locus_tag="VLE22_15560"
     CDS             30274..30531
                     /locus_tag="VLE22_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15874.1"
                     /translation="MTKTTPAHMQTDTAGNLLASVRRDLGIEFDLIGQDLDCFQDLQA
                     LRGIMCRLHELINRIIRIEFAFQSGCHSDQRPDSKGPNLCQ"
     gene            30528..>30878
                     /locus_tag="VLE22_15565"
     CDS             30528..>30878
                     /locus_tag="VLE22_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15875.1"
                     /translation="MKCSVCTSPNRSEVDILLVRNDSNRRIASQYGLTEASLRRHKSG
                     HLPKELTRATEVAELVRADTLLDRVRALCIRSENLLTESEEILADARKSKQTADALNA
                     IRTAAVAARELRAQF"
BASE COUNT         6005 a         9341 c         9651 g         5881 t
ORIGIN      
        1 tgcaggttgg tgatatcaat catgttcggg tcgccaaccc tgatgcccgg caggggcgta
       61 tggaacacgt cgtaccaatc gacccgtttg ccaggctcga cctcccacca gtccatcatc
      121 atcgtgtatt cgagggccag cgggacactt ccgagctggg acgatagcat ctgcgagttc
      181 ggtacggtcc agtaatacat gccgtaggag ccacgtacta cagtcttcct gtccagcttg
      241 taagcggcac cgatgcgagg ggcgaaatcc tccttgtcgc cgttcatgag gttgctcggg
      301 taccccgcct tgtccgccgt agtccaactg aggtcgttgg ctgcatagga ggccagcacc
      361 gaaggaggaa cgccgagact ctcggccgga tggccggccg gtgagatcaa ttgcgtggcc
      421 gtctgccact tggacatgtc caacgcgaat atgcggttgg cggactcatt ataggggctc
      481 cagtaatccc agcgcaggcc gtagttcaac gtgagccgcg gagtgaccct ccaactatcc
      541 tgggcataga agccgagctc gccttggcgc aggtagtaat agggacggga gtaattgcac
      601 ctgtagtagc tgccgtaacc cagaaacatg tcggccattc caaggccggt gaagtccatg
      661 gggtgttggc cttcgggatc ccagagcgcg gtccagtcgc cctggtattt gtaccgtccc
      721 tgcccttggc gggccccgcg cgtattgttg cgctcattgg cgtaacggaa gcctgccttg
      781 aactcgtgct ttccgtgcag ccacgtcagg ttgtcttcgg ccatcagctt gttcaggcgc
      841 tcgggcaatt tgttttcgct atcccagagg aagccgttgc cgccggttat ggtcggccag
      901 ccatactcgt cgagggggtt ggagaggccg aggtactggt tggtccactt gacatcctcg
      961 cgcgcgccgc ctctggtact ccgggacctc tgccccgata gcaccaactc gttaagcagc
     1021 aacggcgaga acgtgtgaac gtgggtgatg gttccgttgt aaaccttgtc cacttcgccg
     1081 atggagttgt attggccttc aggcgagacc ggaccgcctc caaattttcc ggaccagttg
     1141 ttgtcagcga tcgtgaacct acccgcgagg gtgtccttgc ccccaatgta ttggttcacc
     1201 ttgaagttgt aactgttgta ggaagccgga tccgcggatg tcgcgaagta gttgttctcg
     1261 gccagcgggt tggccggtgt ggtgggacga ggtgtgtttg cggagaggta gctgaccagc
     1321 ttgttccctc ccaaagtgga aggaatcatg ttgccggtaa agggcgtgcg ggtgccgctc
     1381 gccgtcgtcg agtacgggtc gtaaatcgtg taggggtggc cggctccgtc gtcgagattc
     1441 gagaaatcgc ccttccacat ggcttcggtc ggcacgatgt cttgagtcga cgagtattcc
     1501 cgcagacgca tgccctggaa tgagaagaac cagaaggtcc tgtccttgcc gttgtacagt
     1561 ccggggatgc gcaccggtcc accggcggac gccccgaatt catttcgttt gtaagggtca
     1621 gggttgttgc catcctggcg cgcccgcgcc cgtaacccgg ccgcgttgtt gcggaatttc
     1681 tcgaagacgc tgccgtggaa ctcgttggtc ccgctcttgg tcaccatggt gatcgtggcg
     1741 ggatgcgaga actgggcggt ggaattggtg tcaacgccga attcctgaat cgtgtccaac
     1801 gccagattga cgcgtggcaa cccaccgcgc atgcggtcga caatcgagcc gccatcctgc
     1861 aagatctcgg ccgcccctgg gttcataccc gccacgtggg gcgagtagga ccccgcttcc
     1921 acgccgggtg tgagctggaa caattgcgtg atgtccatac cgttgagcgg catgtctcgg
     1981 atgcgctcgg actcggtcac gctcgcgatg ctcgagctct cggctgcaat cactggggtg
     2041 acggccctga cttccaccgc ggcagccaga tcgccgattt ccatgcctgg gtcaatcacc
     2101 gcgagttgtt gaacctgcag ctcaaggttg ccggaccact tcttgaatcc ctgcgcttcc
     2161 accaataact ggtacttgcc tgggaacgtg ttcgggaagc tgtagagccc gttctcatcg
     2221 ctttgcgcct gccgcacgga tcttgtctcg acgtttagca gggtcacggc tgcgctcgga
     2281 acagcagctc cgctggggtc cttcaccatt cccctcacgg taccggcagt ggactgagcc
     2341 cacacccccg tgcaggcgac aagcgccagc aggacgacga cagtaatgct tcgatagtta
     2401 ttcatgtcaa atgacctccc tgtagactcc tgcatggacc gccgagagag ccgggtgact
     2461 ttggcagtca aatggaggac gcctgaaact gttgcattgt cttgtgaaaa ctttcacaac
     2521 aacgtgaagc atatcatcaa atattggtcg tgtcaagagc agatcccgtt tttcagtgat
     2581 ttttgatgag ctgggaatcg gtttctcagt ccccaaaaac cgatgtaatc atcaacaaat
     2641 gaaggcggct cactcgcaac ctcacgactc tgcgaaatgt ctcttgaaaa cagggaacag
     2701 atctgataaa ctgactccgg ttgagtgttc tgatgctaac cgggaaaaat gccacatgag
     2761 ttcgcaaaag aaggtcaccc tgcgggacgt tgcccggctg gccaaggtca gcccatccac
     2821 cgtctcgcgg gtggtgaccc gcagcgcctg ggtgagcccg gagatcgagg cccgcgtgag
     2881 aagcgtcgcc cggaaacacg gcttggacct ggaatcgaag atcaaagcgc gggtcatcgc
     2941 cttcgtgctc tccaaccgcg acatgctgca ccccttccat tcgcgggtcc tcgttggggc
     3001 ggaggcgtac tgcaccgctc atggctggga cattttgttt cttaccttcc gctacccagc
     3061 cggcatcccc gcgagtgacg ttcgcttgcc ggacgtgctg cgcaggcggg acttggtcag
     3121 ggcagtgatt ctggcggggg ccaattcccc caacctcctg ggcgcgctca aggatcaggg
     3181 tctgccgttt gcggtgctcg gcaacaacgt cgagggcgac tggcgcgccg aagactacga
     3241 cgtggtgtgg tccgacgacg tggctgggtc ggtcgaactc acgcgctact tcttgtccct
     3301 cgggcatcgg gacatctggt ttgtgggcaa ccgccggctg ccctggtcgg cccgttcctg
     3361 ccaagggtat tcgcaggtga tgcaggagac cggcttggca ccccgcgtga gcagcataaa
     3421 ctcgctcgac gatgaagacc tcggttatct cggaaccaag tcgatcctgt cgcgcaacga
     3481 accgatcagc gccgtcatcg cgagttccga tcaggccgcg gccggtgtgt accgcgcctt
     3541 acgggactcg cagaagcgcg tcgccgacga catcagcgtc gccgggttca acgacaccga
     3601 ggccaccgta ctccaacctc cgctgacaag tgttcgggag ttcggccagg aaatcggcag
     3661 gcacctggcg gaacaagtga tcaaccgcat tcaccagccc gacctgcccc cgcagaacgt
     3721 catcgtgccg accgagctga tcaagcgtgc atcctgccgc gagttttcgc cggatcgccg
     3781 ggcggacacc ggcaggctgg cggcgccggc ggtgtcgtga gccaccctca cttccttagc
     3841 gcgattcttt gctgagcttt catacggtcg gttgccgccc atcgttgcca tggccgcggt
     3901 ggttgataat cgaggaatca tggaaccgaa gaagctgatg gccgtggatc cgaaaacgct
     3961 gccgtgggag gaagtcttta acgagccgac gggcaagtat ctgtatcgcc ggagcctggt
     4021 gacggatccg gataccggga tggagatcaa gatcgtgcga tacccggcgg ggttcacgaa
     4081 tccgctgcac acgcatcact gcgcgcacgg gatgtatgtg cttgagggaa cactgaagac
     4141 gcacgagggc agttacggcc cgggatcgtt cctttggttt ccggagggga tgacgatgga
     4201 gcacggcgcg acggcggagc aggatgtcgt gatcctgttc atcacgaaca agccgttcga
     4261 cattcagtat ctgtgagaag gcacacgggg gcctgggcgg cgggggaaat agagaaggat
     4321 ttttcgtcac ctgcgggccg gggtcctacc ccggcccggc gggcaaggaa gtgtcggctt
     4381 cacaagggga ccgatcccac cctggcgcgt cgatggcggc acgatgtccg ccaccgcaag
     4441 ccctcacgag cgccgcggtc agagtccact gagatgaaac gtacggtggc caccaatacg
     4501 tcgaggcgag tccgtagacc cgccttcctg atttcagtgt aggggcggcg gcgcgaagcg
     4561 caagtcgggg agacgcgatg ttgttgggaa gacagaccaa aaaagatgtg gtttcttgtg
     4621 aatgacggag cgcgacgctc actgcaccac tgtggtgcac cttttggtat cctgtaggaa
     4681 tgcaggcacg acgtattaag gtcggagcta agtccaggaa aggaaggccg gttcgacaga
     4741 gcgtgacgct gccagcggca ctggcatcgg atgtccgccg cgttgccaaa gagcgtcgtc
     4801 tgaccatgag ccgggcactt gttgcgctgg ctgaacgtgg tgtgcaagca gaggcggaag
     4861 cccaggaaca gctcaagacc gcgtaccggc ggtttctcaa cgagaacgaa cccgcccgca
     4921 agaacgaggc cggcaaggaa ctgatcagag ccatcttcgg aaaggacgcg attggcgaag
     4981 atcaagtgct ctgaattggt ggttagtgcc catttcttag cgggatgcta tattcgcctc
     5041 atgtgggatg tactgcacaa cactgcgttc agtgatggtg cgctcttccg gtcctgaata
     5101 cctggctgct gagaaggaga accttcagat gaaccccgtt gtgtctcgtg gcttttgctt
     5161 tgcgatcctg atcgtgctgt cggccgcggg tcttagcgcg gcggagttcc tgccacttgc
     5221 tcccggcaac aactggacgt atcgggacgc ggttaccggc tcaaacttca ccgtcgaaat
     5281 cggcttggtt cagatgtacg caaacaatca cgtgtatcac atgctccgcg gatacacgcc
     5341 gatgccggtg tgggtacgga tcaatgagta tggcaacgcc gttttctggg acgaggcaca
     5401 acgaagcgac gtcatgctga ctgccttcga gaccggtttc gggggatggt tcgaggccgg
     5461 ggctcgggaa tgccccgagc agggacaggc tcaggagaaa cggggcgttc accacggccc
     5521 ggccggcgaa tggaacgtac tcgagatcca gtaccgaacc ttagaatgtg cggacacggg
     5581 cgatttgctg gaacagttcg ccgagaatat cgggatggtg cgccgggtgg tgaacaccat
     5641 cgcaggcccc cgaacgttcg atctcgtcca cgcccgcctc ggcgccctct ccatttccgc
     5701 gggcaatgcc ggtggcttca gtgtcaccgc gacgctaagc ccggaacccg gctattggga
     5761 ggccacgtta cgcgttgacc agcggtccgg ggacagtctc aaagtgcgct tccccacgtc
     5821 gcaggaatac gatctgcgat tgcgtgattc gggcggcaac gtcgtgtgga catggtcggc
     5881 gggcaaggtt ttcgatccac ccgaacacga actccatctc ctcgacggct ggaaagcgtc
     5941 ggtcaaggtt ccgattcccc ttgtaccccc tcagggacca cagacctaca cgctggaggc
     6001 atggttgacg gtcgccgacg gagagccgcg cttcgccgca gccaccacag tgaaggcgcc
     6061 tggcaccact ggcgttgtgg cgctgcgtca gggacggatc aaaatacaac cacccggccg
     6121 ccgcaagaat tgacgactgg cctggaccgc tcttctgtgc gatcccacaa aaagcgaacc
     6181 tgacgagacc gttgttctga aatttgtgac agaatcgggg gcggactcga gatgccggcc
     6241 gccggtctga ccggctggaa aggaatacta tgcccgattc catctcacga agatcattat
     6301 taaaggcgtc tgctatcggc gctcccgcag cctttaccat tgtgaagcct catctggttc
     6361 gaggggccgg gaaggagaaa ctgaaggtcg gcctggtggg ctgcggcggg cgcggcacgt
     6421 ccgccgtttt cgaactgatg gctgccacgg acaacgtcga actcacagcg atggcggacg
     6481 tgttcgagga caagctggag agttcgctcg agcgcctaag aagcgatcgg aacctgaaac
     6541 gcttcgccgg ccagacagtg atccgtgacg ggaagcctta tcagctcacc gtggaggata
     6601 tggcgaaaaa actccaggcg ggcattaagg tggctcccga ccgtcacttt gtcggccagg
     6661 acgcgtttaa aaagttactt gcgaccgata tcgacgccgt gttgttgatc acacctccgg
     6721 ggcaccggcc gctgcacttt gaagccgcca tcgaggccag gaaacatgtc ttcctcgaga
     6781 agcccgtcgc gaccgacccg gtgggtaccc ggcgcgtcat cgccgcgggc cggaaggcta
     6841 aagaactcgg cctcactgtg gtttccggca ccgagatgcg gtacaccccg cagtacatgg
     6901 agaccgtgga gcagatccag gccggcgcca tcggcgacat cacggcggcg tatgcctact
     6961 ggtatggaac tgcgatcttc catgccagcg agaggaaacc cgaatggaat gagatcgact
     7021 ggcaacaccg gaactggtac tcgtttgttt ggatctgcgg cgaccagctt gtggagcagc
     7081 aagtgcaccg catcgacctg gtcgactggg tcatgaagtc gcacccgatc aaggcgatcg
     7141 gcaacggggg acgcgcgtgg cggcgcaacg acaacccggt acacggagac gtcttcgaca
     7201 tcatggacgt cgagttcacg tatccgaacg gggtgaagct catcagcatg agccggcact
     7261 atccgcgcta cgaccacctt cctatgcgaa acggagcgga ggtggtcggc gccaaggggc
     7321 ggagcaactg catggacatg gcgcgcacga agacggagat catcggcggg atgcaggagc
     7381 aggtgcggct gatcaattcg atccgcggcg acgggcccta catcaacaac acggaagagg
     7441 tggccgagag caccatggtc tgcatcatgg ggcgcgaagc ggcctactcg ggccaggaag
     7501 tcacgtggga gcaggtgatg aattcacaac tcgacctgta tccgaaggac ctgagcccgg
     7561 gcgccgagat cccagtcccg aagccgcgcg tgccgggaga gtacaggttc gtgtaggggc
     7621 ctgacgagtg acggtggaag ccgaggacag ggccgggccg gccgtcctgc cgaatatcag
     7681 cggaccgcaa tcgggaacgc ggcgctggag cgcgaaccta cgagaagcac caccggaacg
     7741 gcattccccg gttgcacgtc gaggggaatg cgcaggttca cttgcatgag accggcgacc
     7801 atgcccggag ccgcgcccgc atacacgacc ttggcagact gcccgccgat gctggcctcg
     7861 accgtgagta cgggcttggg ccagctctcc agggcgagtt tgccgtccac cccaggagga
     7921 ttggtctggc cttcgccggt ggcatagagc acaatgtcct caccgcgtgc cgcgggtctt
     7981 aactctgagt tcagggactg atcggagttt acggcgacca tgaaaagacc cggagccgag
     8041 ggcgccacat cgagcgtcac cggccgggac gccacgccct ggtactcgac gatgacctcc
     8101 gtagtggatg ccgcggctac cgcgtacggc acgatcgcac tgacttgcgt cgcgaacgag
     8161 tggacgacgg gcgcggcaac gccatcgaac aggacgcgcg tgccgccggc agtcttctca
     8221 agcaaacccg tcgaatcgaa gcctgccagg gtcagtgcgg ccggccccag ccgctctccg
     8281 aagatggtca cgatctcccc gggggcgact gggcccggtg cgaaactggc cgcgttcact
     8341 acacctgttc gggagaaact gggcgcgtcc tccgcgtaat agcgtgggga aagatcgtgc
     8401 gcgggccacg ccgcgatgtt ctccgcggcg gatgtcagcg cagactgcat ctccctccaa
     8461 tagtcaccgg ccacgacgtg gccgttaaga accacagcca gacagattcc gcccggcagg
     8521 cggcccagga aggctgagga actgtacggg gttccaccga aatgccacca gtaggcttcg
     8581 ggcgctgccg tgcgtacgtg ccatccgagc ccgtaatagg tatcctggcc cgaccacaca
     8641 ggtggactcg gaggggagag catcagacgt acgatctccg gcttcagcac cgcgggttta
     8701 tggagaccgt cgagagcgct ggcgaagcgc accaggtcca tggccgatgc gatccaacct
     8761 ccgctggcgt cgatgccctc catcgagaat gcaccgtagg ggaaaggaac cagtccctcg
     8821 ccgtacacgg aagtgtacag cggcatgggg tcgctgtagt agcgaacctc gccgggggcg
     8881 cggtctctga gtctggtgcg ccccaggcgc atccggctga tgcccgacgg agcgagcacg
     8941 gcctgctgaa catagtcggc gtagggctgc ccggaaacct tctcgatgat gcggcccaac
     9001 acgcaatacc caaagctcga gtaggcgtaa tccgtaccgg ggtcgaaatc gagcggccgg
     9061 cccatcatga agcgaatgat cgtctccggg ctggcgggac gcggcacgcc gagcgcatcc
     9121 gaggccatgt ccgtcatcgg tggcctcatc gggtcattac tgttagcaac tccgttcttg
     9181 tccttcacaa aaccgcccgt atggtgcagg agctggcgga ctgtgatctc aaaaaggcga
     9241 ggatccgggt gggtattggg cggcggtgtc agatgatcga gtaggcggaa tgccttggct
     9301 tcgagatcga gtttgccttc gtcgacgagc ttcagaatcc cggcggctgt gagggtcttc
     9361 gagacgctgg caatgcgaaa gagggactcg ggttcgacgg gctcgcgggc ttcgacatcg
     9421 gctagcccat agccacgagc gaaaaccagc cggccgtctt tcgcgacggc gagagacgcg
     9481 cctcggatgt tccagcgatt caggtagtcc ggcatccact gatcgaagct ctcgagttcg
     9541 gggactgcga tgcccgtttg ggcctgagac cgaaggggca gcagacacag cgccagccag
     9601 agcagagccg tccatcttcc ccacgcaaag accggtttgt ggacgaggcg gactgaggtg
     9661 aggcctgcgc cactacgcag cgacaggctc atagacgcac tccttgccgg tttcgaagag
     9721 cgccaggatg ttttcgggcg agacatccgg ttggatgtta tggaccgagg cgagcacgta
     9781 gccgccgccc ttgcccatga cgtcgaaggt gcgccggacg gcggcgcgga catcatccgg
     9841 cgttccgtgg ggcagaatgt gctgactatc gatgccgccc caaaaggcca tgcgatcgcc
     9901 gaactcggtc ttcagccgct ccggttccat gttcttcgcg ctcacctgga cggggttgag
     9961 agcgttaatg ccgatgtcga tgaagtctcc gatgtagcga gagaccgaac cgcaagtgtg
    10021 gtagagaatc ttcgcgttgg tgtgcgcacg catcgtggca acgatgcgct cctggtaggg
    10081 acggatcatc ttccggtaca tcgggagcga gcacacgggc cggtcctgga gtccgatgtc
    10141 gtcgccgaag ccgacgacgt cgatcaggtc gccgacttcc cgcagcgccc gcgtgttcat
    10201 ctcgatgagg ttctcgagca cggcattcat cagacagtga aacaggtcgt ggtcttcgcc
    10261 gaggtcaaga taccagtcat ggaagccgcg cagatactgc gtctggtgca cgaggtggaa
    10321 gcgggcattc aggcagatgg cgcaatcggt ttgctcgcgc agccggaggg cccgctcgcg
    10381 caggccacgg aagcggccgg ggtcggttgg gtcggggaac gggtggcgcg cgatgtcggc
    10441 ggcggtgatg cggccggcga gcgggctaaa cgtcatgtcg taataaggac agccaggcgg
    10501 ccggcggcgt tcgacacccc actcatcacg gtagcctcca tcttcaagtt cgacgtcgcc
    10561 gccacggtcg ggcgaaccca gcgcaaccgg gcggacgtcc acgtccagat attgaaggac
    10621 gcgttcgtcc agctcgtaaa gctgctgcat ccgatcgatg agaggcccgg cgttgccgaa
    10681 gcccaggtgg gcacataaac ggtcgtacgc ccctttcacg atgtgggtaa aacgcgcgct
    10741 gccgaggtcc atgggcacgc ggtccggttc acgatgttcg agcgcggcaa gcactcgttc
    10801 ccgatgagtc accgaatgat tcctccgagg ctattgtatg cccagtcact ttcggaaggt
    10861 tgtggaaggg gggaccacgt catcgactcg ctccagttcg gctgcgagtt tccgggtcag
    10921 cacgtcaacg tttggctcga gaaacatggt caagtgatcg ccaggcacag tatgaaccgt
    10981 caggccgtcc cgagcagcac cgtcccagcc ataacttggg tcttccgtag ggcggccggc
    11041 cgcgcggaag agcgtcagcc gcccggggta agtctgatgt tgatagttgc tgatccgcaa
    11101 gcggatgccg gaggggtgat gtatgccaga gaaggcgtca ttagcgcgct cgtccggatg
    11161 caggagtgcg cgcagtcctc ccacgacata tccacctagc gccaccatcc gctcccacac
    11221 gtgcgccggc acttctttca tcgcgagttg gcagacacta gccaactgga atctcaccct
    11281 ccagcgcagc ataccgagca ggcgcttggc tgtgaggcgg agcgagcgcc tcctcaggag
    11341 tgcgggattc ggggtgtcga acaacgccag aagggcgacc ctctcgcctg cagccgtgag
    11401 ctgctgcgcc atctcgaagg cgagcaaacc gtcggcgcac cacccgccga ggtggtacgg
    11461 accagaaggc tgctgctgcc ggacgacaga caccagttgg caggcgacac ttggcacatc
    11521 ggacccgccc gccagttgtt cgagccttgg catcaccacc cccagaaacg gacgctctgt
    11581 tcccaaccgc ccagcgaggg gccggaatat cggcagcggc cgcaccatga agaaggccgg
    11641 ctttgagccc tccggctgaa tggccacgat ccgttcacgc tgcgcgtccg ctgattgcag
    11701 cagcacggcc aggcggcgag ccgtcggcgc ggcgagtagg gcatcgtgcc gcagtctgat
    11761 tccgaaggcc tcctcgatgc gcgccagaat gcgagtcgag gccagcgagt gcccgcctaa
    11821 ttcgaaaaag tccccgtcga tgccgggagt cggtattccc agtactgccg cccagatttc
    11881 cgcaagcctc cgttcgcagt ctgttctcgg cgcgacggcg gcggccgcag tcgctcgggc
    11941 tctctggaga tcggcgaact cggcggccag ccgcacccgc tgcacttttc cagaggcgcc
    12001 tcttggaagg ccctccacta tcatgaattc acgcggcact ttgaaaaccg ccagccgcgc
    12061 ggcaaggaaa tcgcgaagtg cgctctcgtc gaacctttcg cctttgcgca ggaccaccgc
    12121 cgcggccaga tcctccccta attgcggatg aggaattgcg aatgctgccg catcggcaac
    12181 tactggatgc gccatgagcg cgcggtcgat ttcctggagc tggatctttt ccccacctcg
    12241 gttgacgaac tccttgagcc tgccggtcac gtacagaaag ccttcgtcat cgaagcgtcc
    12301 gaggtcgccc gttcggaacc aaccgttccg gaaggcggcg cgcgtggcct caggattgtt
    12361 ccggtatccc cgcatcacgt tcggtccacg caccacaatc tctcccacgt gcccggcggg
    12421 cagcaggttt ccggcctcgt ccatgatctc aacgcccggc acgatccgta tgccggcgga
    12481 tcccgccttg ctagcgtaag gcacaagagg agtgctggtg accgcgccca cttccgtcaa
    12541 cccataccct tccaggaccg gtacgcgcag agtctcctcc aattcacgca gcaaggcggc
    12601 tggcagcgcc gcaccgatcg atcgcacgaa gcgcagccgg gaccgcatgg ctaactccgg
    12661 cttctcgcgg cagaggccga ggatcgtgcg atggagcgcc ggccccgctg tgtaccatgt
    12721 aggctgtagt tcgtcgatcc aaaccggaaa ccgatccgcg tcgaatggcg ctgcacagac
    12781 cacgctgccg cccaccatca actgtgcgaa cgccgagagc aatccctgta gatgaaacag
    12841 cggcatcatg ctcaggaatc ggtcggtgcg gctcagcatc attgcttggg cgctggcgag
    12901 cgccatcgct tcaatattgg cgtgcgtgat ggaaacacac ttcgcgcgac ctgtggtcgc
    12961 ggaggtcagc agtgaaagcg ctacggcgga ggggtccggg atgtccgaag ccgcaatcgc
    13021 ctgattcaaa ccagtaagcc tgaacacgcc tgcgccatgt tccggagcgg cttgtgattc
    13081 gagaatcgcg attcccagcg cctccgccgc tccgaccgcg ggagaccggg tgcccgcggg
    13141 aaccagcagt gccgacggac ggaggtcttc taggcaagac tcgaattcct ctgcccgaaa
    13201 agccgggttc aggggcgcaa agcacgctac ggaggatatg ccgaggaaag cgtagagccc
    13261 ctcggcgtcg tccggcaaca cagccgccac cacgttgtcc ccggcgaagc cgccgcccgc
    13321 aagagcccgc gcgagtgatt cgacgtgggc cgacaagcct gcgaaagtga gcggcggccg
    13381 acccggcgcg agaatcgcgg gcgcgtccgg catctctgcg gcgtgaagcc gcagtgtcgg
    13441 agccagagcc atgtgttccg tcattggatt tcgaattgcc ttccgagaac cgcgatctgg
    13501 cgcccacggt ccgatacttc gactctgggc ggagcaccgc ccagggcaaa aacccaccac
    13561 acgacgggtg gcgcgggcgc gacaccggct accaccgtga gctggcctct cgatcccggc
    13621 aggcgccgaa tggcgtcatc ggaaagtgga acctgcggag aggccagcca gagttccgct
    13681 tccggagtgt gcagcaggac tgctccgtcc tgcggccaca gcgccgcctc ggggtgtgcg
    13741 tgccagtggt aggacaacgt ttcgacgctc tctccctcga cgtggtccgc cacgacgacg
    13801 agattcctct tgctgaccca cactgtgcga accaccgatc tgactttcgc cgcgggcgga
    13861 tagcacccag ccagttccac tgcggccgag agaacgtcgc cggttttccc gagacgcatg
    13921 aggcgcggca ttttcctgtc ctgtgcgatt ccgttgatga gcggataatt gtgcgccgcc
    13981 ggtccaatgg tgaactcccg ttcgatgtcc tgcatgtact gctgataacc gggatcggag
    14041 agaatccagc ggccccgcgt tccgatcacg acagtgccgt tgtcgagttg caggtgaccc
    14101 atcggcgagt tcgaacacga taccgctgcg gccaagtcct cgctctccca cccggttcgg
    14161 agcaccagtg cgtagtgcgc gttgagcgct cccgcttccg gcgccacgcg tcgaggcagg
    14221 ctcgcagacg cacgcagtgc tccgagcgcg tcggcgcgaa tccattcgga ccgccaccgc
    14281 tccagatacc atgccctcaa cgggtccgcc tggagactgg cgagcttcgc ctgcgccgag
    14341 aagtgaaacg gcatctccct aggctcgacg tcgcccagtt cggcaacctg ctccatcgca
    14401 ccgggagccg agagcatgta cgactcctcg agaaagtgtt tcaaatgggg atgctcgagg
    14461 atctctttcc gttcgttcag cggaagagcc tccaaccagt cggcgacgaa gtcgagaatg
    14521 taaccgtcat aggccacgcc ctcggtatac ccggtgttcc ggaagtcgag aatcgcaccc
    14581 atgatcgcct ggatacgttg gttcagagcg gctcgggccg gatgtccagc cgcggcggca
    14641 gccagcgccg ctccgacggt accgatcaac gggatattgt gcagccagcg aggaacgtct
    14701 ggctgtgaga gaagatcctg cctggtgcga atgccgcggt agtgggagtc cgatgccggc
    14761 gcgacctctt caacgtgccg gacgcaggcg gcgcgaagct ttttgcgaag ctccgcgtcc
    14821 ggccaacgcc atttcgtcag cgcgctccac agcaggcgcc ccgcgtggcc cgctcccagg
    14881 tccggctgct catactgccg gaacttcggc cattccgaca gagcctccag gtctcgggcc
    14941 gccagccgga catattccgg gttaccgaac cattcagccg cccagccaag tgaattcacc
    15001 cgttcttcca gctcccacaa cagccagacc gtccacggat aatccgaaat cgtaggcacc
    15061 ggaacggaag ggaggacagc gctccggagc ggcaacggga aggccagccg cctgcgaatg
    15121 cgatagtagt cgacaacgag gcgctgctgc cccgcgtagc ttgccgcgcg ccggcgcatc
    15181 tccgcatcga ttccttccct gccggacagc gggctcgcag taagcggcct cgaaacgggc
    15241 aatgcggttg caagtccggc ggggcacagg acaagttgcc ttctggacaa ttcgtggctc
    15301 atgtgtttac gcccaaatca cctcggattc ttctctgcca acagtcggtc tgtcaagttt
    15361 cgcccgctcg ctcacgcttg cggttccata cctactgatc ccatgtttgt agaataggga
    15421 gactttcatg tactcgaaca ttctgatctc gatcctgatc ctgctggtcg cgttccaggt
    15481 tatcggcgga atgctgtcga cgccaaagct gatcaaggcg gcgagcgtcg gcattgtact
    15541 tttggttgcg gcagcgctca tcatatggct gctgatgggt ctctctccga tcccgccttg
    15601 gatctcgaag ttcgtcgtca agatctaccg ccaagccgcc gcggcggccg caagcctcga
    15661 caaggcgaag tgaatcttcc ggaaccccgc gcccactccc ggtgtctact tagtcggctg
    15721 ccgtaagtag tggctggaac cggcagccgg ggcgaagcgc taggaggagg cagccatgtt
    15781 cgagcaagcg tttgtcgaag gtgtcggcag aacccgaagg ccatggactg ttgtcgtttc
    15841 gttcatgggc caagtaggag tagtcggcgt cgcagtggtt ctgccgctcg tttacaccga
    15901 agtgcttccg cggacgcagt tggtgagtct cttcttcgcg ccctcgcccc cacctggacg
    15961 gcgggcgccc cagcctgttc ccgcgccgaa ggctggcgtc aagataccgc cgcgggaatt
    16021 cgacggccgt aagttgacgg agcctggcgc cgtgcccgag aaggtagcaa gcatcatcga
    16081 tatcgaggaa cctccactgc cgtatgcagg ccccgggagc gaagtgggag tcgtgggcgg
    16141 catcgatctg ccgccagagg cgctaagtcc ggcagtgcgg gatgcactca attccggagc
    16201 ggccgcgcgg gtggctccac caccgccggc ttcaaaacca cccgaagaga agatcgttcg
    16261 tctccgcgtc ggtggtgtcg tgcgcccccc tgtgccggtc tatgagccgc gtccagtgta
    16321 cccacagatc gcgcgacaag cgagagtctc gggagtcgtg cacctggaag cactgatcgg
    16381 cacggatgga acggtccgcg gactccaact cgtcagcggg catccgctcc tcgttcaggc
    16441 ggccatggaa gcagtccgca cctggcgcta cacgccgacg ctactgaacg gcgatccgat
    16501 cgaagtggtc atgctgatcg acgtgagctt cacgctcagc aagtaacgat acactggttg
    16561 acgtgtcgaa gaagctcacg gcacacgtga aagcggcggg ttgagcgtcc aagctgagtc
    16621 caaaggtttt ggaccaggtg ctcgcgcggc tcccacgcgt ggcgaatgaa aacgtgctgg
    16681 tcgggtttga tacgtcggat gacgccggcg tgtacaagct gacgcctgag tgcgcccttg
    16741 tgcagacggt cgatttcttc acaccgatcg tcgatgaccc ttacaacttc ggcgcgatcg
    16801 cggcggcaaa ttccctgagc gacgtgtatg cgatgggggg caagccggtc tccgcgctgt
    16861 cgatcgtggc ctatccgggg aagggggacc tcgacgatct ggagcagatc ctgaagggtg
    16921 gtgccgacaa gattcacgaa gcgggctgcg tcgtcatcgg cgggcacagc atcaccgatg
    16981 acgagatcaa gttcgggtac gccgtcacgg gtgtcgtgca tcccgacagg gtgaagacca
    17041 acggcggagc gcgccccggg gatgcgcttg tgttcaccaa acgaatcggc accggagtga
    17101 tttcgacggc gctgaagcgc ggcatcgccc gggaggaaga cgttcgcgtg tccgtcgagt
    17161 ctatgcttgc gctgaaccga tccgcctgcg aggcgatgct cgagttcgac gttcattcct
    17221 gtacggacgt cagtggtttt ggactcatcg ggcacgcgcg cgagatggcg ctggccgggg
    17281 gcgtgacgct ggagttacag gcggaagcgg tgcgattcct gccgggcgcg gtcgaatacg
    17341 ccagagccgg agcgatcccc ggcggtctca agaacaaccg tgagttcgcc tcgtgcgtgg
    17401 tcgagaaaac ccggccgatt tcggacgaga tcgagaatct gctttacgac ccccaaacct
    17461 ccggaggact gcttatcgcc atgccggatg acgatgcggc ccgcttcatc acaaagtgcg
    17521 agtacgctta ccgaatcggc ctggtgctcg cacggcagga gaaggcaatc cggctggcgt
    17581 aaaaccggct acgatatcgg ggtgcgcatc ctcctcgtct ccctcgcttg cttcgcagta
    17641 gccggcgctg tttccgccgg gacactctcg gtcaagatcc tcgacgagaa cggctccccc
    17701 gccccggcgc gcgtctatct gacagactcc tccgggcagt tgcaggttcc ggccggtatg
    17761 atcgtctata agaggactcg aaatgtgtcg gaggaacatt tcgttccgcc ctccggagcc
    17821 ttcaagatcg atctgcggtc cggcgtgtac catctcgtcg tcgagcgcgg gaaggagtat
    17881 ctgccggccg gcgagaatat ccgcgtatcc ggcggcgccg ccgagaaaac cgtccgcttg
    17941 cgccggtggg tccggatgac ggatcgcggc tggtactcgg cggatatgca catccaccgg
    18001 ccattactcg atctcggcgc gatcatggag gccgaggacc tcaacttcgc cgtcaacatc
    18061 acccgctggc gggtcggcga cagggagatt gccgaagatc cggagctgga tgcgttccta
    18121 gcaaagagcg acgccaacgg tgcgatgaaa tccgcgaacg agcgctggtt cacggtactg
    18181 aacgaggagc tcgagccgcc agaaagcgcg ctcctcgtct cccgcatggg caaagtgcga
    18241 agcaccctcg agtatccgat ggcggcgtac ggccgtgcgc tgcggcagcg cggtgcgctg
    18301 gtcgattccg aaaaggcgac gtcgcttgag cttccggcga ttgcggcgct aggcggctgc
    18361 gagacggtcg gccttgccaa caaccatctc tggcggtccg gttccttccc cggtgcatgg
    18421 ggcgcgtggc cggaccggat gatgcgtgca tacccggccg attgcagcgg ttttgctctg
    18481 gcgggtttcg agatgtacta cgccttgctc aatgcgggcc tgccgctgaa gctgtcggcc
    18541 ggcagcgcca acggcgtgca tccggtgccg cccggatgga gccggatcta cgcgcacctg
    18601 gacggcgagt tcacggtcga gcgatggttc gaggccgtgc gggctggccg caccttcgtc
    18661 accaccggac cgatgctgct tctcaccgtg aatggcctcg ggcccggcca ggagcctcgc
    18721 ggcgcgaagt ttccaatgaa ggcaaaggtc aacgtcacgg tgctttcacc cgacccggta
    18781 cgggaagtgg aacttgtagt gaacggcgat gtgcgcaagt tgccgctccg cggttccgga
    18841 atgacgtggt ccggggcgag cgatcttgaa cttacaacca ccacgtggat cgccgcccgc
    18901 tggatgtccg gtggcgacaa ggtgtgcggc gtggcgcaca cggcgccggt cctgtttcgg
    18961 aacggctcgc aaccggtttt cgtccgccgc accgaggcgg agtatcttgt gaaggccgtc
    19021 gaacgcctga tcgacgatgt gaaagccgga cgcaaaggtc caggcgacgc cattccgatg
    19081 atcgtcacgc cgaccccggt agtccgcgag caaacgctcg ggttgctcga gcaggcccgg
    19141 gacttctatc gcggcaagct gtagtcgcgg tccgctcgtg gtcgagcccg gaacgatatc
    19201 ctaagtttga aactacatgt catcgatcac tcgacgagaa gcactcggca tgttcgccgc
    19261 gttcgcgggc gagcctgttt cggcggacca aattcggagg cgcctgccga aagacactcc
    19321 ttccggagtc gccgccatcc tcgcggcaac gctgaattcg gaccccgcct cgcacaacac
    19381 cgactggttc ggtacccttc tgatgaaagg actgcttgag tggggccggc gttatgtccc
    19441 ggaggcaggc ccattcgcgc ggacgtggct cgaagcgcat ctcaacaggg acactgtaag
    19501 cccgtatagc ggccacaggt cccggcccgc tcgtgccggc ggcgttccga ttacgacgta
    19561 cgccggacag ttcggactct gcttcccgtg ctacgagatc tacacacagt cgaaggacga
    19621 gcgcgctcgc agagtctgca tcgagcttgc cgacattatc ctgcatgaag tcgcgcgcaa
    19681 ccggttcgga ctcgtggcgc acgacgacac cgcggagttc gcaatcccgg acacctgcta
    19741 cttcgcggtg agcgctttga ttcttgcgtc ggcgctcgac cggacgggcg gcgcggtata
    19801 tcgccgccag gcggtctttc agcttcgcac gtacacggat acgttcctca tgccggacac
    19861 gggcctggcg aagacaattc tcctcaaaga cggtctcgga aagacttact ggacacgtgc
    19921 gaccggatgg cttctctggt cgatcaccag cgtgcttggc cacctgccgc cggaagatcc
    19981 ggcgttcgcc gggtttgtgc gggatgcccg gaagctcgcg gacggcatct caagggtgca
    20041 ggacgagagt ggcggcttac gcctgttctt gaatgaaccg gacagcccgc ttgaaactac
    20101 cgggaccgcc atgtgcgcca tgggccttca cgaggcgatt cgcaggcggt ggctgccggc
    20161 gtcttacacg ccggtggtgg agcgcgcctg gaaatacgtc accgggaaca tcaccccgga
    20221 gggcgacatc cggcgcgctt acacgggctg ggctgtcccc gccgaaaatc acgaagtgga
    20281 actcgatcag cgcaaaatgg gctggatccc aggcttcatc ctcagcaccg cttacgagct
    20341 tacggcagcg ggaagaacgt aagaccgagt tacagccagt tctaagagac tcgcggtctg
    20401 agacgccggc ccatcgatgc tggctcgaat gtaaaaaccc gtcgtcaaat gttaactctt
    20461 gcaggtaagt attgctcgtg cgcgccgttt ctggtgtact tatgcgaaga gtggcgatcc
    20521 ggctgtctgc ggaagccgga agcaatcagc tacgttcact ctctctgggg gaaacgatgc
    20581 gaaaggtttt tgctgttgtc tcattggggc tggccgttct gccgctgctg ctgcacggcc
    20641 agacgacgga gacgcaacgg tttcaggcgt tgatgagctc gacgcaggag aacagcgcgg
    20701 cgttagtcgg gtaccaggcg atggggagtg ccgttgtgga gttcttcctg aagcgcgatg
    20761 cacagggcgc tctcctgtcg gccgtcacgg atttccgaat cgaacacacg tttggccagg
    20821 cggagaccgt gactaacatg cacattcacc gcggagcggt aggctctccc ggaccggtcg
    20881 tcgtgccctc ccttttcggc gcggcagtgg acgtgaccgc gggttccggc gccttttttc
    20941 gcgcagcgca gaccaccgat cctgtgatat tggcggtgat tgaggagata ctcgccaatc
    21001 cgggcagatt ctacgtcaat gtacactctc tgacgttccc tggcggtatt atgcgggggc
    21061 agttggagag ggatccggct agggcggctg cccgcaggga cgaggcgctg gcggcagaga
    21121 tcgagggcat caaggagtac gagcttcttt ccggccgaat cctccggctg atcggactgc
    21181 gaatgggtgt cattgcgccc tagtagccct tatgcatgcc ctggatctct gatactcgcc
    21241 tcggggcggc cttcggcaaa cggctcaggt ctggagacct gcctacaggg tggcgcagga
    21301 ctccagtcct gtggcggcgc gcctcgcccg cttgcggcaa caccacggga accgtcacgc
    21361 gaccttcaca cgctgggggc tagcagcaac gggatcaacc cactaaggga accggtcccg
    21421 cttgcatgga aacatccttc ctccgtgctc tctgtgtcct ctacggtgaa aatttggatg
    21481 tgaaatgacg ccgagatctg tggcacgatg aacaaacggc agtgcaccga gccacacgga
    21541 tgccgaaacg aaccacgatg tgcggttgcg tagctgttgg ctgcgtcctc tcctgggctg
    21601 gccttcggga cgcagctctt gcacagcaag aaagcccgcg agaaaaggtg cttgtgcggg
    21661 cgacgaatcc tgatcccaaa acccttccta gcttgtatgt cttccaaagg ccccggaact
    21721 atcccaggga catgaggagc atgaccttgg tcgcaagcac gttcccgaac gtgccggatt
    21781 tcacttgcga ctcgtggtgc tatgaaatcc cagtagactt cacgggcacc gcggcgatta
    21841 gcggtggaag gatcgagctt cgccaccggg ttctccggta cccgcaggtg atcctcgtga
    21901 ccacggtgac acctgaaccg ggggcggtcg agttcgtcgt tcgacccgag ttggacaagg
    21961 gtgcgacggg cgaggtaccg gccggcctgc tgctccccaa cctgtgctgg caactgatac
    22021 gcgctcccgc ttttgcgagc aaaccggagg cctaccccga attcgcgaag cgttgtttca
    22081 ttttcaccga aaaaggcagg acgttcttag gcaaaactac tcggcggaat cttcccaatc
    22141 gccctgagta cgagtcgatg aacaatccag tctgggccca gatgtacacg gcggtgtgga
    22201 gccgttttcc ctccgtattg ccgcgctggt cagttaactt tccaactacc gtctggtcag
    22261 aggcgaacag ccccgaccgc tatacggcgc cggtgatcgg aaccgtttcc agagacggca
    22321 agtatctggc ggcgctggga aacggatcag cggaaacgat gttgcaagcg ttccacgact
    22381 gcctgcacaa caaccctgag tggcttccgc cggccgctcc gccggcgcag cagagttggc
    22441 ggctcaagat ctacgtgatg gaaaacgatc ccgaggcgct cctcaagcgc gtcgcgaagg
    22501 atttcccccg gccgtgatac agccgcaaag gactgggtct caccggtaca gggaggcggg
    22561 ccggtaaccc ggcccgccca agagtttgag tggctagaag tccaacctga gaccgaaccg
    22621 ggttgtgcgt tcgccggatc cttcgtgcgc agaatactgc tggttctgcg tttgcgtaat
    22681 caggcccccc gccccgctgt ttatcgtgga gttcgggatg ccaaaattcg ggtggttgaa
    22741 gacattggtg aaggtcgcct cggcttgcag cctccacttt tcgcgcagcc ggaacgcctt
    22801 aaagaaaccg gcattcatgg accgggttcc gggtccgcgc aggacgttca ctccggagtt
    22861 gccgaagcgg ccgacctgca catcgggcgt accgttgcca tccatgtcgc ctggcacgcg
    22921 gaacgcattc ttgtcgaacc acatcgcgat ggtcgggtcg ctcaggtttc cgtttccgat
    22981 ccggtccgga cggccaccgc tggtaccggt gttcgccggg tcataggtgg agaaggccgg
    23041 tgagaagtat cgcccagtct gaagcacacc gaaggtcgaa atcgaccagc ctccgatgat
    23101 gtggctcagg acaccctccc tcaggaactt ctggttcttg ccgacgggca ggtcccagat
    23161 cgagttgaag acgaaccggt gacggcgcgt ataggcgttg tccgcccact cgggacggta
    23221 atcgtaagcg ttttgggccc cccaaccggc ttcccagtcg tcgtgagaat cggtgaggtt
    23281 cttcgcccag gtgtagccca cctgatactg aagacctgct ttgtacttgc gttccgccga
    23341 gagcgtaaga ccgttgtagt aggcgtttag catgttctct ctccagcccg ccgagctcaa
    23401 ctgcggatac ggccgccgcg aggcgctgta cggggtcgtg ctcggtacta actggttgag
    23461 gtttcgggac gccagaagct ggtgggtctt gctgccgacg taagcggccc ggaggcccat
    23521 gttcccaatc tcccgttcga gagtcacact ccactgctct gtgtacggaa ccgccgtatt
    23581 cgggtcttgg ccgctaaggc tcgccgtgcc ggcttggctt agtaccctcg ggaacatgtc
    23641 aggccacatc caaagcgcct gaccgttggt gaccgcattg atcggcgagc tttccgagcc
    23701 gttgtacggc ccgccgacca tcgatcgcca gagcgctgcc gtgtaggtgt catagaagaa
    23761 cccgaagccg ccgcgcaaga cggtcttgtt gtcggaggtg agcttgtagg cgaagccgaa
    23821 gcgtggagcg aggttgtcct tgtccgtgta ggccagcgtg cgctccggca acccggcttc
    23881 ttttgccgtg atgatgggca ccaggttact tgcgacgaat cccgaaaaca gcgccgcttt
    23941 cgcctgttcg ttcggaacca ccatcttgcc cgtgtacgga ttgaagctga aaatccggtc
    24001 tttctcctcc acgtagggcg ggttcaactc gtagcggatg ccgaagttca acgtcaagcg
    24061 cggattcacc ttgaagtcgt cctggaaaaa gagaaacttg tcccagttgg tgctggtgag
    24121 cagcacgaac ccagccgaga cgcgcgaggc cgaccgtggg atgccgaaca tgaagtccgc
    24181 gtaactgtaa cctgtgtagt tgcctgtgaa ggagacgctg ccgtaagccc gtgaaggaga
    24241 ctggccgaag ttctggccgt agttcctcca gacctcgccg cccatcttca acgtgtggcg
    24301 gcccctgatg accgacaggt tgtcctgcca ctgatcgacc ctctccgcac cgcgttgata
    24361 atcgatctgg ctgatggtct ggaagttcga gatgttgacg atcgggatcg cctggagctg
    24421 cggcaggtcc gccggcaggt tggtcagccc catttcctta accacggtcg ggccgtccac
    24481 gggcccgcta taggggttat acccgcggat caggccataa cggaattcgt tgatgactgt
    24541 cggggagaag gtatgcgtgt ccgacaagat gaggttgcgg gtctgccggc gctgcacgcg
    24601 cggcccgacg gtcgggaggt tgccttccca ggtcccgttc ggcgcgcgca ttgcatcgaa
    24661 ccggccgaaa aacgagttct tcgacgagaa ctggtgatcg atgcggatat cgatctgttc
    24721 cttccgcaaa gtctgcttag cctggtcacg gaaattgctt accaccatgt ccgggtctcc
    24781 gaagttgacg tgcgggtaga accgctcctg tgtcttcaag gcggccgaat tgaggagcga
    24841 cgccggaatc ttgttgttct cgaatggcag gcctgtgagc gggttcttga tcgtcacgct
    24901 catcagcttg cccgccgaat ccttcaggac ggagaagtcg ccggctctca tccggtcggt
    24961 cggcaagctc aggttgaaca gcgctgccgt gtgctggttg aagccctcca atccgccgga
    25021 gaagaacgtc ttgttcttga agaccgggcc agtcacggtg aacccgtagt cgttgagaac
    25081 cgcgaatcct acccggttgg cgaaagggtt ccgagcattg aagccggcgc tgttgtagta
    25141 ccagaaagcg ctcccgtgaa cctcgttccc gccgcccttt gtaatgactc ccacgtaacc
    25201 gggtgctccg aactcagccg aggttccgga aacgtcgacc ttcacttcct ggatgaagtc
    25261 catggaaggg ttggccggcc cgacgatatt gccgtacaaa actgaattag tcgtaatgcc
    25321 gtccaccgtg aagttgttga cagtataacg gctgccggaa aacgagattg agaagccggt
    25381 gcccatgggt tgtactccag gtaccagcga catgaagttg aacaggaagc tgtcccagtt
    25441 gccgcgcacg ttcatcggcg ccttcaggta ctgctcctgc gttttgacgt cggtgacggt
    25501 cgctgtctcg gtagagatga gcggtgcccc tgcctcgacg gtcacagagg atgtcagctc
    25561 accgacggcg agattggcgt cgaccagaac tatggcgttg gccgaaagca cgatcccttc
    25621 gcttacgaat ttcctgaaac ccgacgcttc cgcggtcacg gaataggtac cgggctgaag
    25681 atagccagtc tcgtaagctc cggtatcact cgtaacaacc gtcctcgaga tgttggttcc
    25741 cgtattggtc acgacgacgt tgaccttcgg aatggcggca ccggtcggat ccagcaccct
    25801 gccacggatc acaccgaagg tagtttgcgc ccacgtcgcc cctgcgatga gcaggccgag
    25861 agcgacgagc gttaatagac atcgctttcg ctgaaacata aaaccccctt gctgttagac
    25921 tctaaagccg acgaccaagc tgatcgagtg cagcagccag ttaacctgag tacccttttt
    25981 tggcctttcg ggcggcctca gtataccacc agcggcctgt tctggagccg taaaatgaga
    26041 tcctttttcc tgaatccgcg catggaccac cccggcggac ctggcagcac aaggacgagt
    26101 ggcgggcggc agtatcatag tcccggaggg agtcatggcg atctctcgaa aaaccgtctg
    26161 catcacctgg gtcgcgctag ggttcgggtt ggtcatcgct ggcggcctca cgtcgttgta
    26221 cgtcctgccg gcgatccggc aggtatccga gcgcatcccg ctcagcctgc tgtcggtcgt
    26281 ggcggtcctc gccgggatct ttttgatcgt gaggttcgcg ctgaagttgg aggatgtggg
    26341 tggaccggcc caggaaatcc acatacccgg actagacact gacgtgcgat cgaccgggca
    26401 gtcagcgaaa tgactaggaa ctgcgcgttt gatcgtgaag atggaggcgc tgctggttcc
    26461 tgcgacccca tcgtgaccgc ctggaccagc cagtaaagaa ccggttccag aggaggcggt
    26521 tgggtgtaca atcgacgctg gtcaggaaag ccgtaacgga gatccgggtt aattcacttg
    26581 ttgacgagga gcgagtaatt gacgggagtt ccttcgccgt tttttgggtg ctgccgtcag
    26641 ggcggagggt cgtggagata gttcaggcac gccggcagtc cggctgatgc tgttacggaa
    26701 gggaggtttt catggccaag attaacgcga gccgcgttct cctgggtggc ttgttggctg
    26761 gaatcgtgat caacctgttc gagttcgttt cgaatgcggt gattttcaag gatgacatcg
    26821 ccgcagccat ggctgcgctg aacaaaacca tggacatgag cggcggcacc atggcaatct
    26881 acgtctgctg ggcctttctg atcgggatcg tggcggtctg gctctacgcc gccatacggc
    26941 cccgattcgg tgcgggccta gtgacggctg tcaagaccgg cctagtaatg tgggtcctgg
    27001 tcgctgtgca gacgaccatc agtttcgcgc ccatgggtct gtttcctcgc cggctgatgg
    27061 ccatcggtct tgtcatcagc ctggtggagt tggtgctcgc cacagtgctt ggagcctggt
    27121 tgtacaagga ggaagcggca acttaagaat cgggtcgtgt agactcgcat atcagatcga
    27181 tggccctgcg ggttgacgtg gcgtttcagg cttggcggtc tcgttcgacc cagatagaat
    27241 cgttcttttc ttaacacgac cggttacaat gcttccttcc accgggaaag gaggccagac
    27301 gcgatctgtg agacgaagat cagcccccgt ttgtggcttc agggcggtgg catatgaaga
    27361 gagaaacccc accgtcgctt cctctcgcgt gtcaaaatat cacctgcccg ccaacggctc
    27421 caggctcagc tcgatagtca gacccgcttg cccctgtcag cccgggccgc ctggccctcc
    27481 gtccgtcatt ccttccagat gctccagatc cagacggctg ccggcaccag cgcgaccatc
    27541 atcgtcaaga tgaccacctg gattttctta gtcttctccg ccccatgcaa ctcctggatc
    27601 tccatgaagg acaccccgag ataaaagccg gcgcagaaga tcgtcgctgc gaccagcagc
    27661 tccttaatag gcctacttcc tgtccgaaag gattgtcggc ccgagcgagc ccgccgtccc
    27721 tcactcccgc gcctgtctcg gctcccgcac gaggggcgcg gacggcggga ggtgggggtg
    27781 gacgatcagt ttatagagag agcaggacgg ctgcacggac gggacgctgc atgcctccac
    27841 caggttatcg tagatcgtgt caatccgcgt gttgaacgac ttgtgtgcgt cggcagcaat
    27901 ctgatcctca aactgcgtcg tcagcgcggc ccgtccggtc gccatagcca gtttcgccag
    27961 cgttccagat ccggcgtacg gatcgtaccc cgccgccagc gtcagcatag tgccccaaat
    28021 gtcggcatcg aactcacgat tggttggcac gaaatcgagc tttccggtcc gctgctggta
    28081 ggtgtggccg agctcatgcg cgagcgccca agccagttcg ctcggcgagt cgctgatcag
    28141 ctccgagagc ccaagagtca cgccgatctc gtctccccgg cgcgcatagg cgttgacttc
    28201 cttgtcgtag aggatcttga gctgaacagg ccaggtgatg gctccgagtt gcttcgcacc
    28261 gtcttccacc tgtttaaaca cataccagat ccggtcccag tgcgagatgg gccaggcgag
    28321 tgcacccggt ggtagagcgg agagcgtccc agaggcatcc ccattgaggg accatgcgac
    28381 gaacagatcc ggggtgccag tgatcttcaa aacgccatca aagtcccgat ctctcaggtg
    28441 cggaaacttc tgccagatgt taaatgtcgt gtgaccaaat gaggccactg agacgtcggt
    28501 ggggccaccg atcaccagcc cctccgagtc gtagaccgtc agtcgaacgg tgaccggaac
    28561 agagtagacg ttcgccagcg cgaccccgac aaacctgttc gcggccgatg tgtacaccgg
    28621 ggtgggcaga gtcggctgtg cggtgatttc ctgctgcggg acaccgttgg cgatcgcccg
    28681 gaacgcgaca gtcgcctgca ctggaaccga acacgctgcg accgcccacc ccgaaacgac
    28741 cgtctcggag gcgatccggc cgtggcgctt agaccgcctg aggcaaacgc cgccctctac
    28801 ccgtaccccc gtcacaccct tttcacctcc gggggtgctc tgccccccgt cgagccagaa
    28861 tttccgcctg acaatgtcgg ccgtctctga gcgccgggcg agagtcatcc gaaggcactc
    28921 ctgttgggcg gacttcgttt ggcggaacac accatcgccc tgcggtgccg gcgactgcat
    28981 agggcgcggt ggggcagcaa accggggaac gcaactaggg cgatcggaga ggtatatgag
    29041 aactcgtttg aaatgatgca cttcctaggg cgtcaatgcc cagacgacgt agctttggtc
    29101 ccgtcccagg gtcgccggtg atcaacaatt ccgattgacg ctgggaaccg gcaggtgtag
    29161 cataaaagct gtctcgggag tacaagtcga acgactgaat cccaacaggt aagttgactc
    29221 gagacaaggc atctccttcc gaactgaacc ggccttctta ggagccttcg atcagttcct
    29281 cacactactt tgagctgtga tgatttctgt ccggccggct cggtttccga tcgtccgtga
    29341 ggtccgttac aaggtgataa attcgaaggg cgccgtcgaa atcggcaccg gcgagacgat
    29401 caacatcagc agtacgggag tgttgttcaa cgcgcaagca cctttgcgtc caggcaagcg
    29461 gatcgagctt tccatcagtt ggccggccca actcgacggg aagcacggcc tgaaactggt
    29521 ggcgcgcggc cgcattgtac gctgcgaggg cacaaacgtt gccctggaga tcgagaatca
    29581 cgaattccgg accgtcggcc ggggcctggg cgcactagca ggaatctgat cgccccgacc
    29641 tcaggtagac cacccaagtc caagaacgaa aacagggtgg agcctttctg ccccgtcttt
    29701 cacgtttctg gacccgattc ggggctagca aagaaagatg tatctctcca cgtcttcttt
    29761 cttcagtaat gcagctcggt cgtgagtggg atcgggtttt caagtgcttt tggaacagtc
    29821 ggacaaactt ttttcgatcc gggaccgggg gtgggccaat ggtaaggcag cctggcggcg
    29881 gcaggaaatg gaaaagaggc gaccggggtg cgtccgatgg cctcttggga gtagggcgtc
    29941 tctcgacgac cacgggcgaa cctgcccaaa aagataagag tatcccccga agcatcccca
    30001 cacctttttt cctgttctgc aaagtctttt ggaacgattg gtgagccggc cgggactcga
    30061 acccgggcgg cccattgaac ccacgcaact tactgattcc acgttccgcc aaaaccgcca
    30121 aaaccgcacc aaacgccgaa cggaggtaca cggcgggtac acggagacaa aaatgagaga
    30181 tgaatatccg aaatctacga gacgccgtgc gggctcgccg gccgcgtgtg actctctgtc
    30241 cgtccggaca gtcagcccga caggagtaac ccaatgacca aaacaacccc agcccacatg
    30301 cagaccgaca cggccggcaa tctcctcgcg tccgtccgcc gcgatctggg catcgagttc
    30361 gatctcatcg gccaagatct cgactgcttc caggacctcc aggcgctccg agggataatg
    30421 tgcaggctcc atgagctgat caaccggatc atcaggatcg aatttgcctt ccagtcggga
    30481 tgtcactcgg accaacgtcc ggactcgaaa ggacccaacc tatgccaatg aaatgttcag
    30541 tttgcaccag ccccaatcgt tccgaggtcg acatactgct ggtccgaaac gactccaacc
    30601 gccgcatcgc gtcgcaatac ggtctcaccg aagcgtcgct gcgtcggcac aagtccggcc
    30661 acctccctaa agagctgacc cgagctacag aggttgccga actggtccgg gctgacaccc
    30721 tgctcgaccg ggtgcgcgcg ctgtgcatcc ggtccgagaa cctgctgacg gagagcgaag
    30781 agatccttgc tgacgcccgc aaatcgaaac agacggcgga cgcactgaac gccatccgta
    30841 ccgccgcggt ggcagccagg gagctaaggg ctcagttc
//
LOCUS       DATIFQ010000337        32262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2029869, whole genome shotgun sequence.
ACCESSION   DATIFQ010000337 DATIFQ010000000
VERSION     DATIFQ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 32262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32262
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029869"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /gene="wecB"
                     /locus_tag="VLE22_15570"
     CDS             complement(<1..1076)
                     /gene="wecB"
                     /locus_tag="VLE22_15570"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HSB15876.1"
                     /translation="MSKANTTKRILSLFGTRPEVIKLAPVIQELEDRRDVFETVNVSS
                     SQHTSLLYPLIDLFGICIDHDLRVMRPKQTLNGLCARVLESLDPILERTRPNLILVQG
                     DTTTAMAGALAGFHRKIPVAHVEAGLRTGNALSPFPEEMNRRIVSRLATYHFAPTRGN
                     LDILLAEGVPPDRVFLTGNPVVDSLVTILEKFPQLPVVDELVRGTEGLRRIVLTTHRR
                     ESFGETMVENLRVVRRFVESHSDVAVIFPVHPNPAVTEAARLVLADHPRIRLIPPLRY
                     EEFVVLLAYAWLIVSDSGGVQEEAPTLGKPLLILRENTEVPEAVECGVAKLVGGCPDR
                     LASALEQVHSDGTWIEQVRKTDNP"
     gene            complement(1240..2298)
                     /locus_tag="VLE22_15575"
     CDS             complement(1240..2298)
                     /locus_tag="VLE22_15575"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF020704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HSB15877.1"
                     /translation="MRQHSRSIFVTATQRWLNHRGRDENVSSDANVVYRAASDSVETI
                     AIATVLPTEDHVTTEYGGNTAPIREIPATSPSLGYAVAFRSAWNRVSRAVDGLFSTRS
                     LQTLKRVFARVSERLRTLRPFVRTGDAVDRHSDPTRPLPAADQRVRKEEYKLVQHLFL
                     QSNPPAEPVIVFSGVDHGSGCTSICARAADALATLSNGTVCVVDANFRNPSMHRYFNL
                     DNRQGLSTAARRTIPLPHYLQHLAQRSLWVLTSGPSPTDPEALLASAGLRECLSELRA
                     TFDYILIDSPPLDQYPDASLLGCHAGGIVVVVAANSTRWDSALRVKDSLDAARVPILA
                     AVLNQEADPIPSAVQRYL"
     gene            complement(2302..3399)
                     /locus_tag="VLE22_15580"
     CDS             complement(2302..3399)
                     /locus_tag="VLE22_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15878.1"
                     /translation="MRQSTLLRVAYRSADPEASARVLNTLAAMYLEKHTEMHRPRGAY
                     EFFREQAEQYRQGLRRLEARLSAIASDPEAASPQLLKEATLQRMVQFEADRELNRASI
                     AVAEERIQALERLSTSVPARTVTQIRTSSKAVEDLRTKLLELQLKRTELRRLFQPGYP
                     ELQIIENQIAQTQQAIESQEKAPPTEEVTDRDPTHEWIRSELMKARAELSGLRARADS
                     VGSVLAEYGKKTQKLTQMEIASEELMRQVNLAKENYLSYAKKQEDARISDELDRHRIL
                     NVAIAEAATAPFAPSGPGKAKLMFLAATLAAALSVGLAFFVDSRDPTLRSVEEVQACL
                     NVPVAAVLSGSLLALPAPRQEESSSEPPGRS"
     gene            complement(3914..4669)
                     /locus_tag="VLE22_15585"
     CDS             complement(3914..4669)
                     /locus_tag="VLE22_15585"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HSB15879.1"
                     /translation="MNYRRPVLCMTILLLLASIATPQTTKTSRSTAITPASTSQLGTQ
                     AAIDTPSQAGASRTTTTPFETRHPRYRIRNGDVFTVTFPHVPAFNQTVTVQPDGYVTL
                     QIVGDLYVQNLTVPELTETLVSAYGKTLREPAITIELKDFEKPYFMATGEVGRPGKYD
                     LRGTTTVAQAIAIAGGFTGDAKHSQVLLFRNTADNWTEVREINLKNMLAKKNLEEDLL
                     LQPGDLLFIPKSALSKLRLFSPRMTIGPSIRPF"
     gene            6532..7995
                     /locus_tag="VLE22_15590"
     CDS             6532..7995
                     /locus_tag="VLE22_15590"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB15880.1"
                     /translation="MVSAREAPLLPTVLVLTSRSDVAAYAEEVLGSAGVSVSVINDED
                     GARTIIGKRDSKIGLLLVDVEAPRIDGTGAMQRIHSDWPELPVVILSAENSSIAMEVA
                     LERGASAFVRLPVQRGDLLNRVTELLRAQPGQLPARNDAFGLSAGPFCCTNARMQAIR
                     SSVRRIARFDVPVLVLGESGVGKEVLARQLHVSSHRAGKPFLKLNCAAFPSELLESEM
                     FGYERGAFTGAQKTTQGRLEIADGGTVLLDEIGDMELRLQAKLLHVLQDGEFLRVGGR
                     EPVRVNIRVVAATHQDLQRAIEEGRFRADLYYRLAVLAIEIPPLRERQDEIVAMADYF
                     LRKHCIPGTRSPGITAELREALLAYDWPGNVRELENVMQRLLVLEDTTSIIRELHANM
                     LRRSGNRQMSTSSLHGNEGARPELANRRRSAFEEANQAKATIEAKAIRIALNATHWNR
                     RKAAALLNIEYKTLLYRMKKLGIDGDSEDLLAELKQA"
     gene            8448..11537
                     /locus_tag="VLE22_15595"
     CDS             8448..11537
                     /locus_tag="VLE22_15595"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB15881.1"
                     /translation="MPKSGIPRLFLCSIPWCVVMAHPQSIQSAVKAFPEAEGFGAVAI
                     GGRGGQVIEVTNLNDSGPGSLRACVEASGPRTCVFRIGGNIVLKSKLAVRSPYITIAG
                     QTAPGGGITLRTDPVNSSGLVEIRDTAYDVVIRNIRIRPGPGSGAGNDLDAVTIMGSR
                     VILDHVSASWGVDENVNSWYSTAHDITVQWSIVSEGLNNSTHTEGPHSKGMLLGSQDA
                     HSITAHHNLFAHNDERHPQVKTRTGGVVDVVNNVVYNWGQKAAYTDDSYGIPPVNFVG
                     NYYKPGANSSLGMYEIHHRNAAGRGTELFVKGNIGPHRLDDSLPESEVVRPEDRGYIV
                     ATRHAAPLVTTQTAQVAFELVLAGAGASIALRCDGTGYLRRDTVDTRIVTDVRNGTGR
                     IIDDPSQVGGWPVLEPGAGCVDVDRDGMPDVWESAHGFNPGLASDGAQDADGDGYTNL
                     EEYLNITDPRGGSPPPPSDTTPPVISGVAADGVTSSSADIVWATDEAADGQVEYCMTS
                     TKCGTTTTLEGTLATQHRLHLFNLQAATTYLYWVKSTDASGNLAEAGPMAFVTPSAPS
                     PGSALRVPQDFATIQSAIDTAKDGDVVLVSPGAYRENLVLAGKSVTLASEFYTTQDVR
                     LIDQTILDGGGATVVTVAASAGAETKVIGFTIQNGGDGISAAGKLHILNNRIIGNSDG
                     IDYEGGGGICRNNVFENNSDDGIDFDGPTEATVEDNLIRNNGDDGIEIRLHEYSGPTL
                     EIIVRNNVISGNGEDGIQLIDHPGVSNRVFVIERNLIERNAMAGLGMMDNGVTVEDFR
                     AASVTDPVHLFNNTFRNNPYAVTGGDNLVALNNIFLGSAGIGVKGVDGESIAAYNLFW
                     GNGTDASSSNVDSASTVFADPLLAVDSRLRSGSPAIDAGTAFYQWKGQTVLNLPSNTY
                     SGAAPDLGKFEYIGGGPTPAPLSVTSIMPASMRVGTSVNITISGTGFEPGLQVSLAGG
                     QGSTPTVSRVTVVSSTTVTATVSVGTAGPKRTRYWDVVVVNPGGEKATLPRGFTVVK"
     gene            11739..13259
                     /locus_tag="VLE22_15600"
     CDS             11739..13259
                     /locus_tag="VLE22_15600"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15882.1"
                     /translation="MTSNLNRRRFMEAAAASGVFTIVPRHVLGGAGFVAPSDKITMAH
                     IGMGTQGFNELGGLLTEPEVQIVAVCDPNKGSSDYVEWSKHSIRNTIREILGNPSWRE
                     NDDGCPGGREVGCEVINTYYANQRGSEKFRACASYADFRELLDKEKDLDAVKIMTPDH
                     LHATISIAAMKKGKHVLVHKPIANRMYEARLSIETARRAKVATHLLAYGSGSANAKII
                     GRIKGGVIGKLREIHNWSSRPFWPQYPEIPTDRPPVPEGFDWDLWLGPALDRPYHPHY
                     THTVFRGWYDFGGGSMADMGIYSLWPVLTALDLGAPLSAEAWSTHTCSIVDQVSREAK
                     NDFSFPAACTLRFKFAARGDMPALDLFWYDGGMKPRMPEAIEAANTEIQKEGILFIGD
                     HGTIMAGFNGQSPRLFAKGKSEPLWPEEAEQAGGGRRRRGPGGRSNVWLPSLKGGELS
                     PGNFLNAASISDTVNLGTVALRAGTKVLFDSEAMKITNVPDANKYLYREYRQGWEL"
     gene            complement(13300..15165)
                     /locus_tag="VLE22_15605"
     CDS             complement(13300..15165)
                     /locus_tag="VLE22_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15883.1"
                     /translation="MLISVRCGIVLATVLVLGFAMEAAAAGPVIEVHEPMTPPEWALL
                     EREVLRAGSSAVDVFYDKYFDERGYLLHVARWGILDGTDDAIETFHNWPLFHALGADD
                     LVLDRYRKALEGHLRQYTAVTTVTTDIAKNGVYHREFYGYTDWLHAGEGLRSFFFQGL
                     TDPADPRYINRMRRFAGLYMNENPEAPNYDPRYRIIRSVLNGSKGPLLRKLTARDWIG
                     DPVPGTFHLLHSSEGDRKMLDFQAEYPDMLAAVDDPVFDSAAGDNPLNLATTNLATSA
                     YLLTGDRKYRDWVLEYAGAWRERTQANGGIIPGNVGLDGAIGSAYGGKWYKGIFMWDR
                     DRYEGSLASWGMWPGFGNALLLTGDRAWIDALRRQIDVLYAQGRKVNGRFMVPNNYGD
                     NGWYHPREYIFAHELGKIWAWTMDPRDRDRLPQEGWIAFLAGKDPSYPETAFRKELDF
                     IRQRTDRVRQDSTTPDSRLADWALQQNPVTTRALVELTCGGHRIDHGLDRLFGLLHSR
                     VRYFDPVRQRAGLPTEVAALVTAMDADTFRLTLVNVNQVEPRTVTVQGGAYGEHQIRE
                     VVIAGKTSPVNGRFFTLRLAPGTGAELIVRDTRYANQPTMAMPWYGDLTSAAWAN"
     gene            complement(15209..15895)
                     /locus_tag="VLE22_15610"
     CDS             complement(15209..15895)
                     /locus_tag="VLE22_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HSB15884.1"
                     /translation="MTRRCAFLWTVVMLAGCAREERPAAPAEPAGSAENAAPARSDSR
                     PVIVAFGDSLTAGHGVEPGLSYPDFLQKELDRRGYGYRVVNQGISGDTTGGGVARVNA
                     AVSLRPTVVILELGANDGLRGLPVEATRSNLADMIVAFHRAGAKVVLCGMTLPRNYGP
                     EYIRSFEAVFRDLAREKKTPLVPFFLEGVAMRQDLMQPDALHPTARGNAIVAENVLKY
                     LEPLLKRSPG"
     gene            15935..16633
                     /locus_tag="VLE22_15615"
     CDS             15935..16633
                     /locus_tag="VLE22_15615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB15885.1"
                     /translation="MNSPILSLRGVTKSIDTGAHRVDILRGVDLEIAHGEFVAIMGVS
                     GSGKSTLLGLLAGLDSPTSGSIRLDGTETSRLSEDQLADLRGRKIGFVFQSYHLIPTL
                     TAEENVLLPAELAGESGAEATERARFLLDTVGLKDRREHYPVQLSGGEQQRVALARAF
                     MRKPPVLLADEPTGNLDSTNGRQVLELLLTLNRQEGATLVLVTHDRDLSDNAGRIITL
                     KDGLIVGDERKSTT"
     gene            16630..19173
                     /locus_tag="VLE22_15620"
     CDS             16630..19173
                     /locus_tag="VLE22_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSB15886.1"
                     /translation="MTLRSPAWRMAWREARAVAPKFAFVIFGIAAGVGALTGVRGFSA
                     AFHDALKREARTLIAADLTVRQFLMPTDAQMTAVEPWMHRGVRLTRIMETVSMMGTEA
                     GSNPILVSVKAVDPRAYPFYGKVKLEPARSLPDALTPQTIAVSDDLTVRLNLSAGSTA
                     RLGSADFTVSGVVRFEPDRMTGSLNVGPRVLISREGLDRAGLLIAGSRASHRLLFKLP
                     AQGVDVAEMRAALQKVFPEAMVADYREGHPFLNRALRRSTTFLSLVSLIAMIVGALGV
                     ATAIHSHLQQRLDSIAILKCLGARSAQIIRIYALQTILLGVAGGTAGVAVGAVVQKLF
                     PLLLARYFQIEHIPWNATFALEGIAAGVLVSLLFTIPPLLTIRQVKPALIFRREMAEA
                     KPRWRDRVRMHIPALASGCLILAGLGGIAGWLAESPQMGAYFIGGLAVALLILAGVAW
                     LLLRGLRLFLRRSPIRLPVSLRHGIANVYRPGNHAASVLVALGIGVMFTLTIYLVQKS
                     LLIEVAGAAPPDSPNVFLINITPRDHEAVTQFLNARRDLRAKPRIVPLVPVRLLLVNG
                     KPVEYIEHAGRHWHFRYTRQVTWSESKPDDVELRQGAWWTGGEKTPSVSVSEDTAAAL
                     KIEPGTTLGWLCVDQQFDARVSAIHRVKSVRPGQSDDFIFNRVALDGFPVQWFGAVRM
                     APNRVAGLQREAYQRFPTVTVINAADVLNIVQEVVDQVALVVRFISAFAIIAGAIILA
                     STVAGTRLRRTREAAVLKTLGARRRRLVEIFSVEFAILGAVAGLMGGALATVFSRLLL
                     IRLLDAKFSFDVIPNLATVLLTAALAIITGWLASVRILRERPLEVLRHE"
     gene            complement(19181..20476)
                     /locus_tag="VLE22_15625"
     CDS             complement(19181..20476)
                     /locus_tag="VLE22_15625"
                     /inference="COORDINATES: protein motif:HMM:NF018506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HSB15887.1"
                     /translation="MSPTAKSATLPPALSLHRLGVICGLAAGAWLGAAEAPTKLVTMG
                     YSPFVISVGMVAGVFVARWTVPVLLKGTGYVLLDLREKVHLVVWAVLAGMLWAVANTL
                     TVFAIRNVGLSIAFPLWNTNSLIGLFWGWLLFNELRNAGAKQWAKVLGGGAAMLTGAC
                     LLAWATSHQSAVAPNRAAVGILAALAAGVLWGTMYIPYRKAYISGMNPLSFVTVFTFG
                     ELGTAITLAAVFHGGFHGLLVELDSARSAMFWLFLGGFCWVLGDLFQQYAAKYIGIGR
                     GIPLSNTNQLWGLAWGALVFGELAGLGLTAQILVVAGSLVMIAGAASISFAEAPESEQ
                     ASWRKAMERECDRYGLNRERVAAVLQGDDPLDKETPSRRWWELIFVACALGGFVWLAS
                     GAERQPIAVNPFWIILLAAATLVFLVVCGTLLWKRTRFS"
     gene            complement(20606..21436)
                     /locus_tag="VLE22_15630"
     CDS             complement(20606..21436)
                     /locus_tag="VLE22_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB15888.1"
                     /translation="MFSRRQFLAVAAAPLAAKAPRGLKLGVMDGVLRQSGKPEAIALA
                     KSFGMEGLQVTLGRPTAGADRLPLEDEEVQGRFIAESKKHKLPIDATYIDVLHVNCLK
                     SDPLARTWVLRGIDITKKLHASILMTVFFGKCSVENRAELDYVADVFKELAPEAEKAG
                     VILGFENLLNAEDNARAMDRVASKAFKIYYDAGNATNMVGVDAPKEIRWLGKDRICQF
                     HFKDKSYLGEGKVDYRAVLQAVSDIKFEGYANLETNTPSGSMENDLRRNINYLRKLMS
                     "
     gene            complement(21457..22668)
                     /locus_tag="VLE22_15635"
     CDS             complement(21457..22668)
                     /locus_tag="VLE22_15635"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB15889.1"
                     /translation="MKRYLALFAVLCGVATAADPKFVPVFDGKSLTGWEVCNGLATYR
                     VEKGAIVGTTTEGSPNSFLCTTREYGDFVLEFETKTDPALNSGVQIRSHRYAEGQTYW
                     ALDKGKLAEHKTPPGRLYGYQVEISNEESGTSGGIYDEARRAWLHNIESDPDARKAFK
                     DNQWNRYRVAAMGDSIKVWVNGVPCADLIDSMDLTGVIALQVHSYKGEKPAQVRWRNI
                     RIQDLGRHVWKPLWDGKTFDGWTKRGGGNWTIEDGAIHAVSVAGDTRVGFIVSDQSFK
                     DLTTRIMFRIPKGNSGYFVRCDPKTLAAYEVEIDATKRTGGFWETGQGGRAWVTGPED
                     NAAVKDNDWNELTASLQGHRIVFHVNGVKTLDLPNDTQGRLEGNLALQAHGRMETEVW
                     FKDIAVLVPER"
     gene            complement(22701..24380)
                     /locus_tag="VLE22_15640"
     CDS             complement(22701..24380)
                     /locus_tag="VLE22_15640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HSB15890.1"
                     /translation="MSSLNCTLNRFEEEFAGRHLIHGVVRHWAKEEPDSLAFINADRK
                     QEVDWATFDRITDLVAMQLVGLGFRWGDFFATSLPFLTEHLYLEYACFKIGVIHAPLD
                     LRLKPAEVIRCLNLIGAKGYAFPGTARGIDFGELGRAAKAHCPTIRTLVQLTDPAETI
                     EGAVSFAELAAAAPPDELQAAFNKVTAAVDENDGAQVIFTTGSTGTPKGALLSHRNIT
                     CQNMCLGAAFGFGENTRLLVNLPPSHVGGQAEALMTTLFWGGTAVLLEVFDALRSVEA
                     IQTHRVNILGQIPAMYQFEWRLSDYGKYDLSSLEFVIYGGQQVPMQFLERLAVMSPKI
                     GTGLGLTEAAGFCTYTHLDASPAEIIESIGHEMPVYPMSIRQLMRDDGSAGDELPDGE
                     MGQICFRGPQTFLGYVNDPAATARAISANGHLYTGDMGFRDGKGLHFAGRSKWVIKPG
                     GYQVFPAEVENHLCTHSKIAACGVVGMEHAVFTEAIVAFVEKKSGADLTAKEIKEHAR
                     AMTSYMRPRHYVILEPGQMPLNRGVKTDYLRLQEMAREEIERLRAARRWDR"
     gene            24491..24796
                     /locus_tag="VLE22_15645"
     CDS             24491..24796
                     /locus_tag="VLE22_15645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HSB15891.1"
                     /translation="MIIVHVHVHVKPEFIGAFRKATVENARNSIREAGIARFDVIQRS
                     DDPGRFVLVEVYRTAEAPAQHKETAHYQTWRDAVEPMMAEPRGSVKYANVFPADESW"
     gene            24804..25958
                     /locus_tag="VLE22_15650"
     CDS             24804..25958
                     /locus_tag="VLE22_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HSB15892.1"
                     /translation="MRFEFASAGRIVFGPGSVREIAPAARKMGRCVLLVTGGSPARAA
                     GIARELEAAGVSCTGFRVASEPTVELIRQGTARAHEERCDTLIAVGGGSVIDAGKAIA
                     ALLANGGDPLDYLEVIGAGRPLLRASAPFIAVPTTAGTGSEVTRNAVLASLEHRVKAS
                     LRSAGMLPRLAVVDPELTLDLPPALTASTGLDALTQLIEPFVSVRANPMTDAICLEGM
                     QRACGALARAYRNGKDSEARTDMSLASLFGGIALANAGLGAVHGFAAPVGGMFDAPHG
                     AVCAALLPHAMRVNIRALRARAPESEALRRYETVARVLTGDAAASPEDGVASLERLCQ
                     ELSVSPLRTYGIGDRDVPELVQKAAKASSMKGNPITLSGDELTELLVRAL"
     gene            25998..27296
                     /locus_tag="VLE22_15655"
     CDS             25998..27296
                     /locus_tag="VLE22_15655"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HSB15893.1"
                     /translation="MKRLAWVCVVVSAAAGILPGQRNKDAGLAREKLPSWLKIGGEIR
                     GRLEAERGFKYVPGEDDTYYLHRLTFDMSVHPASWLSFSAQVQDARVAGYSRGPSPSS
                     LANPFDLRSGFVELGRDAAKGWSLRAGRQEMKFGEDRLIGNSNWSNIPRRFDAVRLTW
                     KRQNATLDWFASSVVTTVRDGFDRPRLYNGFFGFYSSFRNLPGKSTVEPYLLWKTNAR
                     VRGELGTFGDLDVYTVGARVVGELPLRFDYGAEVALQRGSYAADDIRAWAGHWRLGHK
                     LPYANKAFRLVLEFNHASGDGKPRDGRHGTFDQLFPTNHQKYGIADRITWRNINDLMA
                     GVEWRPAKKWRWLIDYHSFWLATLQDGMYTDAGALVVRNPGASSRRIGGEVDVQGSYQ
                     FTEHLQFGAGFGHLFAGPFLRESIPERSSSHPYVMWTYAF"
     gene            27381..28661
                     /locus_tag="VLE22_15660"
     CDS             27381..28661
                     /locus_tag="VLE22_15660"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HSB15894.1"
                     /translation="MSLFFSTGSETAELSSADLRNGLFQALDALGKRRKVLCLPPDFT
                     RYHSRAGELTNLAWQYFGERLTDVLPAIGTHFPVSEHEIGLMFGDMPRDRFRVHDFRC
                     GVVTLGEVPREFIREQSEGKLDFTWPAQVDRLLIEGGFDLILSVGQVVPHEVIGMANY
                     NKNVFIGTGGADGINKSHYLGAVYGMERIMGRTDTPVRRVLNYASEHFAGNLPIVYVL
                     TVVGKNEAGDLVTRGLFVGDGHECFEKAAALSLKVNFQMLDREIRKAVVYLDPGEFKS
                     TWLGNKAVYRTRMALADDAELIVLAPGVKEFGEDPAIDTLIRKYGYVKTPEVLQAVEK
                     NADLAGNLSAAAHLIHGSSEGRFTITYCPGGLTKEETEGVNFRYAPLDRMARCYAPER
                     LTDGWNLVDSEEVFYISNPALGLWAYRGRFEQSA"
     gene            28891..31752
                     /locus_tag="VLE22_15665"
     CDS             28891..31752
                     /locus_tag="VLE22_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15895.1"
                     /translation="MRTLTVGLIFFATIFLAAPSALGAGRATLIREGEIGPPAEDGLV
                     RLEQALQTKGIVVDRSDGSQAVYGDVVVLAGLGSGAVAAALNAEDVTLPQGPEALAIR
                     RTTHQGKPAVLLWGSDTRGLMYAALETADRILWAQSASNPFAQIRDVVEEPDVIERSV
                     SIYTMQRAYFESRLYDERYWRRYFDMLSASRLNSFVVIFGYENGGFMAPPYPYFFDLD
                     GFPGVRLTGITPAQQQRNVAAFKAMVRIAHEHGIDVIAAIWDHIYRGGVQGGGIPGAS
                     ENAGKEAPHLVSGVTAENLVPYTKAALLRFLEVFPELDGLQFRMHGESGLKREEMESF
                     WHDVFGIIHEKNPGIRVDLRAKELPDSIINDALGQGLKVRVTTKYWMEQMGMPFHPTH
                     INRQNQRDRRHGYADLLRYPQKYTVHWRLWNGGTTRLLLWGDPEYVKRFASTTHVYGG
                     RSFEINEMLATKMLGSPHNEPPAPILNQSYRYYDYEFERYWHFYQVWGRVSYNPDTPP
                     ETWEREFQRRFGPVAGAQVMDALHLASNVLPRIVAASYLYQHFPTTRGWAEMMRGDDL
                     PKYAGMEGSDIAQFMNLRDAARSLIEGTETAMRRPEETSRWFENVSERITAHVREAET
                     AVGGRAGKELISTLVDLKILAGLAKYHAVRLRAGLSYNLYKETGDLFSFDDAVAQEKQ
                     AVAAWERIVEAAGGVYSENLAFGVHEVGFSRHWKEELQALRRGLDQLLAERQQSRLQG
                     ASLKSRIAHVPVRQSGPGSAIRIRATVALPEPKQVNVLVAGSKGGFRAVPMQRVEAGM
                     YQAEVRPGSADDRLRYFIEVREASGGPSTWPQKGKNDPVVVAVTADRQPPSVWPDPPA
                     KAKPGENLTVTARVQDPSGVGSARLRYRHLTQFEDYHLREMTLDPKTGLYTAQIPGSF
                     ITPEWDLIYFIEALDIHGNGRMYPDLETDTPYIIVSVAR"
     gene            31778..>32262
                     /locus_tag="VLE22_15670"
     CDS             31778..>32262
                     /locus_tag="VLE22_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15896.1"
                     /translation="MREAPGGAPAQGAASASLHSNFAWTFAGNSFYAAGQWVILSLFA
                     KLGGSEMLGQYALAVALTAPVVMLSHLNLRAVLATDVGGQYPFGDYLAVRLGTTAAAL
                     VAIAGITAAAGNSWPLAGSVVAVGVAQCAETVSDIYHGAMQRRERMEQIARSIIARTS
                     VS"
BASE COUNT         6243 a         9734 c         9943 g         6342 t
ORIGIN      
        1 aacgggttat ccgttttacg tacttgctct atccacgttc cgtctgagtg aacttgctca
       61 agtgccgacg ctaagcggtc cgggcagccg ccgacgagtt tggcaacgcc gcactcgacc
      121 gcttcgggaa cctcggtgtt ctctctgagg atcaacagtg gttttcctaa tgtcggagct
      181 tcctcctgca cgccgcctga atcagatacg atcagccagg cataggccag caaaacaaca
      241 aattcctcgt accggagggg aggaataaga cggatccttg ggtggtccgc gaggaccagg
      301 cgagccgcct ctgttactgc agggttcgga tgaaccggga agatcactgc gacgtcacta
      361 tgactctcca cgaatcttcg gaccactcgc aagttttcca ccattgtctc gccaaagctt
      421 tcccggcggt gggttgtcag cacgattcgc cgcaagccct ctgtcccacg caccaactcg
      481 tccaccacgg gcagttgtgg gaatttctcg agaatcgtca cgagcgagtc cacgacgggg
      541 ttaccagtca aaaacaccct gtctggcggc accccctcgg caagcagaat atccaggttc
      601 ccgcgagtcg gcgcgaagtg ataggtggcg agccgggaga caattcggcg attcatctct
      661 tcgggaaacg gactaagcgc gttgccggtc cgcaagccag cctctacgtg tgccactggg
      721 atctttcgat gaaatcccgc gagtgcgccc gccattgcgg tcgtcgtgtc tccctggaca
      781 aggatcagat taggtctagt tcgctccaga atcggatcga gcgactcaag gactcgagcg
      841 cagaggccgt tgagcgtctg ttttggccgc atcactcgga ggtcatggtc aatacatata
      901 ccaaacagat cgatcaacgg atagagcaag cttgtgtgct gagaggaact gacgttcacc
      961 gtctcgaaca catcgcgtct gtcctcaagc tcctgaatca ccggcgccag cttgatgacc
     1021 tccggacgag taccgaagag actcagaatt cgtttagttg tatttgcttt gctcatggtg
     1081 tttacctgta gctctgggtc ccggttgtcg tacagataag taagtgcaat aactgagcgg
     1141 cgccgacccg gagagggctt cgttccggct gagacgttcg tgacgcgccc tcgtgccttc
     1201 ggaacgcttg tctgttctga ccgaaatcaa gtgactgctt cagagatatc gttgaactgc
     1261 acttggaatc gggtcggcct cttggttgag caccgcggca agaatcggca cccgtgccgc
     1321 atccaggcta tctttcaccc gaagggcgct gtcccaacgc gttgaattgg cggcaacaac
     1381 gactacaatt ccacctgcat gacatcccag gaggctggcg tccgggtact gatcgagcgg
     1441 tggtgagtca atcaggatgt aatcgaatgt tgctctcaac tcggacaaac actctcgcaa
     1501 tccggctgag gcaagcaacg cttcggggtc cgtcggcgac ggtccgctgg ttagtaccca
     1561 aagactgcgc tgcgcgagat gctggaggta gtgcggcaac ggaatggtcc ttcttgctgc
     1621 tgtcgatagt ccctggcggt tatccaggtt gaagtagcga tgcatggagg ggttgcgaaa
     1681 gtttgcgtct accacacaga cggtcccgtt gcttaacgtg gcaagggcgt ccgctgctcg
     1741 cgcacaaatc gaagtgcacc cgctcccgtg atccacgcct gagaaaacga tcacgggttc
     1801 cgcgggtggg ttagattgga ggaataagtg ctgcacgagt ttgtactcct cctttctgac
     1861 tctttgatca gccgcgggaa gcggccgggt ggggtcggaa tgccgatcaa cagcgtctcc
     1921 agtccgtacg aacggcctta gagtgcgtag gcgctcgctc acgcgagcga aaacacgctt
     1981 cagggtctgc aggctcctgg tggaaaagag gccgtccacc gcccgtgata cgcggttcca
     2041 tgcggagcgg aatgcgacgg cgtacccgag cgacggagag gtcgctggaa tctcccggat
     2101 cggggctgtg ttgcctccgt attctgtggt cacgtgatcc tccgtcggaa gcacggttgc
     2161 gatcgcgatc gtttcgacgg aatcgctagc tgccctgtat acgacgttcg catcgctgct
     2221 cacgttctcg tcacgacctc gatggttgag ccaccgctgt gtagcggtca caaaaattga
     2281 tctgctgtgt tggcgcacgg ctcaactcct tccgggcggt tcgctggagc tctcctcctg
     2341 ccgaggtgcg ggcagcgcga ggaggctccc tgatagaacc gctgctacgg gtacattgag
     2401 acaagcctgc acttcttcaa cggagcggag cgtgggatcg cggctatcga cgaagaaggc
     2461 cagcccgaca ctcagggccg ccgctagcgt ggcagccaga aacatgagtt ttgccttacc
     2521 tggtccactc ggggcgaagg gagccgtcgc cgcctcggcg atcgcgacgt tcaaaatcct
     2581 gtgacggtcc agttcatccg aaatccgcgc atcctcctgc tttttggcgt agcttaggta
     2641 gttctctttc gcaagattca cctgccgcat cagttcctca gaggcgatct ccatctgggt
     2701 caacttctgg gttttcttgc cgtactcggc aagcacagac ccgacagagt cagcgcgcgc
     2761 ccgcagtccg gacaattcgg ccctggcttt catcagctcc gacctgatcc attcatgagt
     2821 ggggtctctg tcagtcactt cctccgtcgg gggagccttc tcttgacttt caatggcctg
     2881 ctgcgtctgg gcaatttgat tctcgataat ttgaagctcc gggtaacccg gctgaaacag
     2941 gcgcctcaac tccgtgcgct tgagttgcag ctccagaagc ttggttcgca gatcctccac
     3001 cgcctttgac gacgttcgga tctgtgtgac tgttctcgca ggaacagatg tgctgagcct
     3061 ctccaatgct tggattcgct cctcggcgac cgcgatacta gctctgttca gctcacggtc
     3121 cgcctcgaat tgcaccattc tttgaagcgt tgcctccttc agtaactgtg gcgacgcagc
     3181 ctcaggatcg gacgcaatag cactcaaccg tgcctcgagt cggcgaagtc cctggcggta
     3241 ctgctcggct tgctctcgga agaactcata tgcacctcgc ggccggtgca tttcggtgtg
     3301 tttctcgaga tacattgctg ccagggtgtt caatactcgc gccgacgcct ctggatctgc
     3361 gctccgatac gccaccctca ggagcgtcga ctgccgcatt ggttccgctt caagttggct
     3421 gtcaagcttc gatacggcgg cggcgactaa cgcctcttga ctgggaggag tctgctttaa
     3481 gaaagatgca agcagagact gtgaattagc tgaagtacct tgttgttcgt gcaggcggca
     3541 cttcaacacg acactgcgca gaagatctcg gctccgcaac aactgaagtt cagtgttgat
     3601 ctcttcctgc gagatgctca tcagtggccg aggtgctaag gcggaatcag aggtgacggc
     3661 agaatctacc cggcctcggt taaacaacag catcatctcg gcttcaaaag tcggaggctg
     3721 cggcagtacg taaagagccg cacctgtcac gatgccaaga aaggcggagg cgatgagacg
     3781 cgaacgtctg aatacgacat aaacgcaata tcggagagtc ggtacaccag ctcgatatct
     3841 tggagacatc agcgcttttt ggtcgtccat gagtagcctc ggaacaatgg aagggtgatt
     3901 gatgtgacca ccgctaaaag gggcggattg aggggccgat ggtcattcgt gggctgaaga
     3961 gtcgcaactt actcagtgcg ctcttcggaa tgaacaatag gtcccccggt tgcagaagaa
     4021 ggtcttcctc gaggttcttc ttcgctagca tgtttttcag gttaatttcc cgcacttcgg
     4081 tccaattgtc ggccgtattg cgaaatagca gtacctgaga gtgttttgca tcaccggtga
     4141 atcctccggc gattgcaatc gcctgcgcaa cggtcgtggt tccgcgcaag tcgtacttcc
     4201 ctgggcgtcc aacctctccc gtcgccatga agtacggctt ctcgaagtcc ttgagctcaa
     4261 tcgttatggc aggttcgcga agggtctttc cataagcaga taccaatgtt tcggtcaatt
     4321 caggtacggt caggttctgc acgtacagat cgccgacgat ttggagtgtg acatagccgt
     4381 ccggctgcac cgtcacagtc tggttgaacg ccggtacatg ggggaacgtg acggtgaaca
     4441 cgtccccgtt tcggattcga tagcgggggt gccgagtttc gaagggtgtt gttgtggttc
     4501 tggaggctcc ggcctggctt ggcgtgtcaa ttgcagcctg cgtgccgagc tgactggtac
     4561 ttgccggtgt gatcgcggtc gaccgggaag tcttggtcgt ctgtggagtc gcgatagagg
     4621 ctaggagtag aagaattgtc atgcaaagaa caggtctacg gtagttcatt gcagcgtcct
     4681 catttcattg cttctgcgac atcactcccg gcgtgcaggg caggtcaggg gaggtcgcag
     4741 acggaaatcc tgcttctggt ggtcgcccta gatcatcacg gtgaaaccgg ttcgtgagta
     4801 cggacactct cggccgccgg cggatgagct gcgtgctgag gcaaaagacc cgactgcttg
     4861 attacacggg cgagtgcatc ggttttccgg tgaagcgcca taacaactcc tgtcaacacc
     4921 ctgactgcct catccgtgtt gtcttcctga tgtgagccgg agactggccg tctcagttgc
     4981 gtgaaaacgg caggtactcc gaaagagttg agcgtcagcg ggctctggga gttgctgtcg
     5041 agcagacagc acaccgtgga cctgatgaac tcggaaaggc tacgggcgcc ggaggcgagg
     5101 gaaattgagc ggagggcctc atattcccag tcggccacgc gaaacgtgat caggcgactt
     5161 cgtggattga gaatacccat tagaatttac tccagtgagt tccagtcttt cgcagctccc
     5221 ggtcagtgcc gggagattac cgctccctgg aaggatgtgc ctccccactt gaggccagaa
     5281 cagaggcagg gactgctggg ctgtgctcca aaacacgaat tgcggcaggt tgtccgcttg
     5341 ttcgccggac cgaccaacca tcaatctcga ccgccacagc tcgctgaaga agggtcagag
     5401 tgacaactag gcgatccggc ttcttgagcc cactcaggac accctccagt ccggtgagcg
     5461 gccccccatc gatcacgacc tggtcaccca cgcggggcac cggccatggg taggccgcga
     5521 gtccggacct gagaattgtt ttgatggatt caatctcgtt gtcgtcaact ggagctggcg
     5581 tccgccccac gctgaccatc ttcagtaccc ccggtgtagt gagaattgga agccaattcc
     5641 ctgtcccgag ccggcagaac acgtatccag gaaacaacgg cagttgaaga tttactgttc
     5701 tgtcagacca gcggtgcctt gcacgataaa ggggtaggaa tggctcgatc cccttgttct
     5761 gaagcgcgca ggaaaccggc ttctcgtgcc ttgacttcac tcggatggca taccatcggt
     5821 gggttaccgg atagttggac tcgcaagtac tgttgtacgt cactggcgtc atttcgatcc
     5881 ttgagggcaa acacttcgta agctaccgcc ctctcggtcc catagtttgg ctcggtccgg
     5941 agaagccacc cagcagcttc ggcccgatgt actcgcatgt gaatgaccaa cgtattcgct
     6001 ggcgaatacc gttgtcctct gtacgtgctt cactccggaa cgatcggtgg gtcacattcg
     6061 atgtcggcgc ctggttccaa acggtttggc aacaagttag gcgaccggga tgccggccag
     6121 cgctcaaccc gaaaacgtct ctcggtgcga aggtgaccct gcctgtcggc atcgaactcg
     6181 caggcagtgc cgtgacagac cgtgggaacc tttccccaag agtgtcaaag gatggctgcc
     6241 gcgaaggctc cccaacccgt agcgagttca tccgaggctg ggggcggctc gcgctgactc
     6301 tgcgtactgg aaccctttcg gcgtggttcc aagactcaca gaacgcttat gccgtctatg
     6361 gtacttccca gccttgaaat cggtgcgaat cgtattgtaa ttgaatataa cgcgatgaaa
     6421 atgtcagcgg ggttacaagg gtcgactgca cgcttccagg ttgcgggggt cgatgaacgt
     6481 gagaaggggg gtgtcgtgcc aggccagggg cgggacccga accccccgcc gatggtcagc
     6541 gcccgagagg ctccgctttt gccgaccgtg cttgttttaa cgtcgcgctc agacgtcgca
     6601 gcgtacgcgg aagaggtatt gggctctgcc ggcgtctctg tgagtgtcat caatgatgag
     6661 gatggtgctc gaacgatcat aggcaagcgg gacagtaaga tcgggctgct gttggtggat
     6721 gtcgaggcgc ctcgaataga cggaacgggc gccatgcagc gcatacactc ggactggcct
     6781 gaattgcccg tcgtgattct ctccgccgaa aactcctcca tagccatgga ggttgcgttg
     6841 gagcgtggcg cctccgcttt cgtcagactt cccgtccaaa gaggtgacct tctaaaccgt
     6901 gtcacggagt tgctgagggc gcagcctggg caactgccgg cgcgaaacga tgcattcggt
     6961 ttgagcgccg gcccattctg ttgcacgaac gcacgaatgc aggcaatccg ctcgtctgtc
     7021 cgccggatag cccgtttcga cgtgcctgta ttggttcttg gagagagcgg ggtcggtaag
     7081 gaagttctag cgcggcaact gcatgtgagt tctcaccggg cgggcaagcc attcctgaag
     7141 ttgaattgcg ccgccttccc gtcagagctt ctggaaagcg agatgttcgg ctatgaacgt
     7201 ggggctttca cgggagccca aaagacgaca cagggcaggc tggagatagc ggacggaggc
     7261 acggtcctgc tggacgaaat cggagacatg gaattgcgtc ttcaggcgaa gctcctgcac
     7321 gtgcttcaag acggcgaatt cctgcgagtg ggtggacgcg agccggtacg ggtgaacatc
     7381 cgggtcgttg ctgctactca ccaggatttg caacgtgcga tcgaggaagg ccgattccgg
     7441 gctgatttgt actatcgcct cgcagtgctc gccattgaga ttcctccgct gcgtgagcgc
     7501 caggatgaaa tcgtcgctat ggcggactac ttcctcagga aacactgcat tcctgggacg
     7561 cgaagtccgg ggatcaccgc ggagttgcgg gaggcactcc tcgcctacga ctggcccggt
     7621 aatgtccgcg agcttgagaa cgtcatgcag aggctgttgg ttctggagga caccacaagc
     7681 atcattcgag aacttcacgc caacatgttg cgaaggtccg gtaaccgcca aatgtccacc
     7741 tcgagcttgc atggaaacga aggagcgcgg cccgaattgg caaaccgcag gcggtccgct
     7801 tttgaggaag cgaaccaggc gaaggccaca attgaagcaa aagcaatccg gattgccttg
     7861 aatgcgacgc actggaatcg cagaaaggcc gcggcgctgc tgaatatcga gtacaaaacg
     7921 cttctttata ggatgaagaa actcggcatc gatggtgatt ccgaagatct tcttgccgag
     7981 ttgaagcagg cataacattc ggccccggtc tcagagtgag ttccgatctg ggggcggccc
     8041 cttcgccttt ccctcatgcg gacgaggcgc agtccacggc gcggatcgcc ctcatacgct
     8101 ttctccggac actgtgtaac gcttctggta tgatgctctc atatatagat tgaaagcggc
     8161 ttcacgccgt cctgtctggc agaaactaca cattttgtgt ggtaattaca ttcggatcag
     8221 tcgtttgggt actgtcaata ttcccactac ttcgttattc accaacaagt tacggtgctt
     8281 ctaaccgctt ggaccggcac gtgctaagtt tgtcgcctcg cgtaattaag ggggatggtc
     8341 agtcatcgac catctccaag tcatttcttc gcccacgagt ctgacgcccg gcggcgatct
     8401 actaggcgcc tgtgttcggg gtcagacccg agagaaggag tttgaccgtg ccgaagtctg
     8461 gaattccacg cttgtttctg tgttccatcc cctggtgcgt cgtgatggcg cacccccagt
     8521 cgatccagtc agccgtgaag gcattccctg aagctgaggg ttttggagcg gtcgccatcg
     8581 gcggccgcgg cggacaggtc atagaagtga ccaatctcaa cgactctggc cccggcagcc
     8641 tgcgcgcttg tgtcgaggca agtggtcccc gcacctgcgt cttccgcatt ggcgggaata
     8701 tcgtgctgaa atcgaaactc gcggtccgga gtccctacat tacaatcgcg gggcaaacgg
     8761 cgcctggcgg cggaatcacc ctgcggacgg atccggtcaa ctccagtggt ttggtcgaga
     8821 tccgcgacac ggcgtacgac gttgtcatcc gcaatattcg gattcggccg ggcccgggtt
     8881 ccggggcggg gaacgatctc gacgctgtca cgatcatggg gtcgcgagtg attctagacc
     8941 acgtctcggc gagttgggga gtggatgaga acgtcaactc ctggtacagc accgcgcacg
     9001 acatcaccgt tcagtggagt attgtctccg aaggattgaa caacagcacc cacacggagg
     9061 gacctcacag caagggcatg ctcctaggta gtcaggacgc tcacagcatc acggcacacc
     9121 acaacctttt cgcccacaac gatgagcggc atccacaagt aaaaacccgt accggaggag
     9181 tggtcgatgt ggtgaacaac gtcgtataca actggggaca gaaagctgcc tataccgacg
     9241 attcctacgg aattcccccg gtgaatttcg taggaaatta ctacaaaccc ggcgcgaata
     9301 gctcgcttgg gatgtatgag attcatcacc ggaacgcagc cggaaggggg accgaattgt
     9361 tcgtgaaggg aaatattggc ccccaccgcc ttgacgacag cctgccagag agtgaggttg
     9421 tcaggcccga ggaccgcgga tatatcgtgg ccacaaggca cgctgcccct ctcgtcacaa
     9481 cacagaccgc acaggtggca ttcgaactgg ttctagcggg cgctggagca agcattgcac
     9541 tgcggtgcga cggcaccggc tacctgaggc gagatacggt ggacacgcgg atcgtcaccg
     9601 acgtccggaa cggcaccggc cggatcattg acgacccatc ccaagtcggc ggctggccgg
     9661 ttctggagcc cggcgccggt tgtgtcgacg ttgaccggga cgggatgccc gatgtttggg
     9721 agagtgccca tggcttcaac ccaggtctgg cctccgatgg tgcgcaggac gctgacggtg
     9781 acggttacac caatctcgag gagtacctta acatcaccga cccgcgaggt ggctcaccac
     9841 cgccgccatc cgacaccaca ccgccggtca tttccggagt cgccgctgac ggcgtgacat
     9901 cttcttcggc ggatattgtc tgggcgaccg acgaagcggc ggacggccag gtcgagtatt
     9961 gcatgacctc gacaaagtgt gggactacaa caacgcttga aggcacactc gccacgcagc
    10021 acaggctaca tctttttaat ctccaggcag cgaccaccta tctttattgg gtgaagtcta
    10081 cagacgcttc tggcaacctc gctgaggctg ggccgatggc tttcgtaaca ccctccgccc
    10141 cttcgcccgg aagtgcgctg cgggtaccgc aggacttcgc tacgatccaa tccgcgattg
    10201 acaccgcgaa agacggcgac gtggttctgg tgtcgcccgg cgcctatcgg gaaaatctgg
    10261 ttctagccgg aaagagcgtc acgctagcgt cggaattcta cacgacgcaa gacgtccgtc
    10321 tgatcgacca gacgatcctc gacggaggcg gggctaccgt cgtaacagtg gcagcctcgg
    10381 ccggcgcgga aacaaaagtc atcggtttca cgatccagaa cgggggtgac gggatcagtg
    10441 cggccgggaa gctccacatt ctgaacaacc gcattattgg caacagcgac ggcatcgact
    10501 acgaaggcgg cggtggcata tgtcgcaata acgtgttcga aaacaacagt gacgatggca
    10561 ttgatttcga cgggcccacg gaggcgactg tcgaggataa cctcattcgc aacaacggag
    10621 acgatgggat agagattcgg ctgcacgagt acagtggccc caccctagaa atcatcgtcc
    10681 gaaacaacgt catctcggga aacggtgagg acggaattca gttgatcgac caccccgggg
    10741 tgtccaaccg ggttttcgtc atcgagcgca acctgatcga gcgcaacgca atggcaggcc
    10801 tcgggatgat ggacaacgga gtgacggtag aggacttccg ggcggctagc gtcacagatc
    10861 ccgtgcatct tttcaacaac acgtttcgga acaatcctta cgctgtcacc ggcggcgaca
    10921 acctggtggc gctaaacaac atcttcctcg gttcagccgg gatcggggtg aagggcgtcg
    10981 acggagaatc catcgctgcc tacaacttgt tctggggcaa cggcacggac gcctcgtcga
    11041 gtaatgttga ctcagcatcc actgtgttcg ccgacccgct actcgccgtg gacagccgct
    11101 tgcgatcggg aagtccagcg atcgatgctg gcacagcgtt ctaccagtgg aagggacaga
    11161 cggtgctgaa tcttcccagc aatacctatt ctggtgccgc gcccgacctt ggcaagtttg
    11221 agtacatcgg tggcggtccg acgcctgctc cgctgtcggt gactagcatc atgcctgcta
    11281 gcatgcgtgt cggtaccagc gtcaacatca cgatcagtgg cacaggcttc gaaccggggc
    11341 ttcaggttag cctcgccgga ggccagggat ccacacctac agtgtcgcgg gtcacggtgg
    11401 tgagttcgac caccgtaacc gccacggtga gtgtgggcac cgccgggccg aagagaactc
    11461 gctactggga tgtagttgtg gtcaatcctg gcggcgaaaa ggccacgctg ccgcgcggtt
    11521 tcaccgtggt taagtaaaga gcgactgtaa gcgtcacagc cattgtcccc ggaggcgcgg
    11581 cggttcccac ccaccggccc cggacaggcg acttcgtgta cactgggacc cgccgggagg
    11641 aaacgacgtg ttcggatgca gtcaccgagc cgcgaccgga gggagcggaa gcagtgaact
    11701 ctttatagca ccccgccgga tcccgtggga ggcattcaat gacatccaat cttaatcgcc
    11761 gccggttcat ggaagccgcg gccgcatccg gcgtgttcac catcgttccg agacatgtcc
    11821 ttgggggcgc cggcttcgtc gcgccgagtg acaagatcac catggcgcat atcggcatgg
    11881 gcacgcaagg attcaacgag ctcgggggct tgctcaccga gcccgaggtc cagattgtcg
    11941 ccgtctgcga cccgaacaag ggcagcagcg actatgtgga gtggagcaag cacagtattc
    12001 ggaacacaat tcgagagatc ttgggtaatc cctcctggag ggaaaacgat gacggctgtc
    12061 ccgggggcag ggaagtcgga tgcgaggtca tcaacacgta ctacgcgaat cagcgggggt
    12121 cggagaagtt cagggcttgc gcttcatatg ccgatttccg cgagttgctg gacaaagaga
    12181 aggatctgga cgccgtcaag atcatgacgc ccgaccatct tcacgcgacc atttccatcg
    12241 ccgcgatgaa gaagggtaag cacgtgctgg tgcacaagcc catcgcaaac cggatgtacg
    12301 aagcgcggct gtcgatcgag accgcgcgta gggccaaggt agcgacccac ctgctggcat
    12361 acgggagtgg ttccgccaac gcgaagatca taggccggat caagggaggc gtgatcggca
    12421 agctccgcga gatacacaac tggtcgagca ggccgttttg gccgcagtat ccggagatcc
    12481 ccaccgacag accgcccgtg ccagagggtt tcgactggga tctgtggctg gggccggcgc
    12541 tcgaccgccc ctatcacccg cattacacac acacggtatt tcgcggctgg tacgactttg
    12601 gcggcgggtc catggcggat atggggatct acagcctctg gcccgtgctc acagcattgg
    12661 acctaggcgc gccgctcagc gccgaggcgt ggtcgaccca cacctgctcc atcgtggatc
    12721 aggtcagccg ggaggcaaag aacgatttct cgttcccggc tgcctgtacg cttcgcttca
    12781 agttcgcggc gcgcggcgac atgccggcgc tcgacctgtt ctggtacgac ggtggcatga
    12841 aaccgcgaat gcccgaagcg atcgaggcgg caaacaccga gatccagaaa gagggcatcc
    12901 tgttcattgg cgatcacggc acgatcatgg ccggcttcaa cgggcagagc ccgcgactgt
    12961 ttgccaaggg gaagagtgaa ccgctatggc cggaggaggc tgagcaggcg ggcgggggac
    13021 ggcggcgaag aggtcccggc ggccgcagca acgtctggct cccatctctc aagggcggag
    13081 agctgtcgcc cgggaatttc ctcaacgccg cttcgatctc ggatacggtc aacctcggaa
    13141 ccgtcgcact gcgggcgggg acgaaagtgc ttttcgacag cgaggccatg aaaatcacca
    13201 acgtccccga cgccaacaaa tacctgtacc gcgagtaccg gcagggttgg gagctgtgac
    13261 cattccccgg ctcgcatcgg cgggacctga cgcccgatgt cagtttgccc aggctgccga
    13321 ggtcaggtct ccataccagg gcatggccat ggtcggctgg tttgcgtagc gagtgtcgcg
    13381 aacgatcagt tctgcgccgg tgccgggggc gaggcggagc gtaaagaagc ggccgttgac
    13441 ggggcttgtc ttccccgcga tgacaacttc ccggatctgg tgttcgccgt aagcgcctcc
    13501 ctgcacggtg acggttcggg gctcgacctg gttcacgttc accagcgtca gccggaacgt
    13561 atcggcgtcc atcgcggtca ccagcgcggc cacttccgtg ggcaggcctg ctcgctggcg
    13621 cacggggtcg aagtatcgta cccggctgtg cagaagccca aacaggcgat cgagaccgtg
    13681 gtcgatccga tggccgccgc aggtgagttc caccagcgct cttgtggtca cggggttttg
    13741 ctggagggcc cagtcggcca gccggctgtc cggggtggtt gagtcctggc gcacgcggtc
    13801 ggtgcgttgc cggatgaagt ccagttcctt gcggaaggct gtttcaggat aggacgggtc
    13861 cttgcccgcc agaaaagcga tccagccttc ctgaggcagg cggtcccggt cccgcgggtc
    13921 catcgtccag gcccagatct tgcccaattc atgagcgaaa atgtattccc gcggatggta
    13981 ccagccgttg tccccgtagt tgtttggcac cataaaccgg ccgttcacct tccggccctg
    14041 agcatagagc acgtcgatct ggcggcggag ggcgtcgatc caagcgcggt ccccggtcag
    14101 gaggagtgca ttgccgaagc cgggccacat gccccatgac gcgaggctgc cctcgtagcg
    14161 gtcgcggtcc cacatgaaga tgcccttgta ccatttgcca ccgtaagcgc tgccaatcgc
    14221 accgtcgagc ccgacgttgc cgggtatgat gccgccgttc gcctgcgtgc gctcacgcca
    14281 tgctcctgcg tattcgagca cccagtcgcg atatttcctg tcgccggtca gcaggtaggc
    14341 gctggtggca aggtttgtcg tcgcaaggtt caaaggattg tctccggcgg cgctgtcgaa
    14401 gaccgggtcg tcgacggcag cgagcatgtc ggggtactcg gcctggaaat cgagcatctt
    14461 ccggtcaccc tcgctgctat ggaggagatg aaacgtgccg ggcaccgggt cgccaatcca
    14521 gtcccgcgcg gtgagcttcc ggaggagcgg acctttggag ccgttgagaa cactgcggat
    14581 gatccggtac cgaggatcgt agttgggagc ttccgggttc tcattcatgt acagcccggc
    14641 gaaccggcgc atgcgattga tgtaccgcgg atcggccggg tccgtgagtc cctgaaagaa
    14701 gaagcttcgc agcccttcgc cggcatgcag ccagtcggtg taaccgtaaa attcgcggtg
    14761 atacacgccg ttcttcgcga tgtcggtggt caccgttgtg acggctgtgt attgacggag
    14821 atgcccctcc agggccttcc gatagcgatc cagcaccagg tcgtctgccc cgagggcgtg
    14881 aaagagtggc cagttgtgaa aggtctcgat ggcgtcatcc gtgccgtcga ggatgcccca
    14941 gcgagctacg tgcaacaggt agccacgctc atcgaagtat ttgtcataga agacgtcgac
    15001 cgcactggat ccggcccgca gcacctcccg ctcgagaagc gcccattccg gcggcgtcat
    15061 cggctcatgc acctcgataa ccggcccggc ggccgccgct tccatcgcaa atcctaaaac
    15121 cagcaccgtc gccaggacga tgccgcaccg cacgctgatc aacattgctc gctccgctag
    15181 ttggttttct tttccggatc cggaagattc atcctggcga acgcttcagg agaggctcca
    15241 ggtacttcaa cacattctct gccacgatcg cgttccctct tgccgtaggg tgaagcgcgt
    15301 cgggttgcat cagatcctgc cgcatcgcca cgccctcaag gaagaaagga accagcgggg
    15361 tctttttctc gcgcgccagg tcgcggaaga ccgcctcgaa gctcctgatg tactccgggc
    15421 cgtagttccg aggaagggtc atgccgcaga gcaccacctt cgctcctgcg cggtggaaag
    15481 cgacgatcat gtctgccagg ttcgaccgcg tggcttccac cggcagcccg cgcaggccgt
    15541 cgttcgcacc caactccaga atcacgaccg tgggccgaag actcaccgcg gcgttgacgc
    15601 gggccacccc gccgccagtc gtgtcaccac tgatcccctg attcaccacg cggtaaccgt
    15661 accctctccg gtccagttct ttctgaagga aatctggata gctcagcccc ggctcgacgc
    15721 cgtggccggc ggtgagactg tcaccgaagg cgacaatcac gggccggctg tccgatcgcg
    15781 ccggggccgc gttttccgcg gaaccggcgg gctcggccgg ggcggcgggc ctttcctccc
    15841 gtgcacatcc ggccagcatc acaaccgtcc acaagaacgc acaccgccgt gtcatcacct
    15901 gtcatcatag ttgaaggaca cgatcacagc tcggatgaac tcccccattc tttcgctccg
    15961 tggtgtaacc aaatctattg acacgggcgc gcaccgcgtc gacatcctcc gcggagtcga
    16021 tctcgagatc gcccacggcg aattcgtcgc gattatgggc gtatccggct ccggcaagtc
    16081 cacactgctc gggcttctag ccggcctgga ctcacccact tccggatcca ttcgactcga
    16141 cggcacggag acctctcgcc tgtcggaaga tcagctcgcc gatctccgcg gccggaagat
    16201 cgggttcgtc tttcagagct accacctcat cccgacgctg accgcggaag agaatgtcct
    16261 actgccggcc gaactcgccg gcgagagcgg agcggaggcg acggaacgtg cccgctttct
    16321 tctcgacacg gtggggctca aggaccggag ggagcattac ccggttcagc tttcgggcgg
    16381 cgagcagcaa cgcgtcgccc tggcgcgcgc gttcatgcgg aagccgcccg tgctgctggc
    16441 tgacgagccc acgggcaatc tggattcgac gaacggtcgc caggtactcg aactccttct
    16501 cacgctgaac cgtcaggaag gcgcgacgct cgtgctcgtg actcacgacc gcgacctttc
    16561 ggacaacgca ggcaggatca tcacactcaa agacggtctg atcgtcggtg acgaacgcaa
    16621 gagcacaaca tgacccttcg ttcgccggcg tggagaatgg cgtggcggga agcgcgcgca
    16681 gtagctccga agttcgcgtt cgtgatattc ggaatcgctg ccggagtcgg agcgctgacg
    16741 ggtgttcgcg gcttttccgc ggcgtttcat gacgcactca agcgcgaagc gcgaacgctc
    16801 atcgccgccg atctcacagt ccgccagttc ttgatgccca cggacgcgca gatgacggcg
    16861 gtggagcctt ggatgcaccg cggagtccgc ctcaccagga ttatggaaac cgtttccatg
    16921 atgggcacgg aagcaggctc caaccccatt ctggtttcgg tcaaggcggt cgatccgcgc
    16981 gcatatcctt tttatggcaa ggtaaagctc gagcccgcgc gttccttgcc tgacgcgctc
    17041 acgccccaga cgatcgccgt ttccgacgat ctcacggtgc ggctcaatct ctcagccggt
    17101 tccacggccc gcctcgggtc ggccgacttc acggtgagcg gcgttgtgcg cttcgaaccc
    17161 gaccgcatga ccggctcgct gaacgtcggc cctcgcgtcc tcatcagccg tgaaggcttg
    17221 gatcgtgccg ggctgctgat cgccggctcc cgcgcgtccc accgcctcct gtttaagctg
    17281 ccggcacagg gagtcgacgt cgcggagatg cgagccgcac tgcaaaaagt cttccccgag
    17341 gcgatggtgg ccgattaccg ggaaggacat cctttcctca accgcgcgct ccggcgatcc
    17401 acaacgttcc tgagtctcgt ctcactgatc gccatgatcg taggcgcgct tggcgtcgcc
    17461 acggcgattc actcgcacct ccagcagcgt ctcgacagca tcgccatcct caaatgtctt
    17521 ggcgcgcgct ccgcgcagat catccggatc tacgcgctgc agaccatttt gctcggagtc
    17581 gccggcggta cggccggtgt cgcagtggga gcggttgttc agaagctctt ccccctgttg
    17641 ctcgcaaggt actttcagat agaacacatc ccgtggaatg ctacgtttgc cctggagggc
    17701 atcgccgcgg gtgtcctggt atcactcctg tttacgatcc cgccgcttct gaccatccgg
    17761 caggtcaaac cggcgctcat cttccgccgt gagatggccg aagcgaaacc gagatggcgc
    17821 gatcgcgtgc ggatgcatat tccagcccta gcatccggct gcctgattct cgctggtctt
    17881 ggcggcatcg caggctggct tgcggaatcg ccgcagatgg gagcgtattt catcggcggt
    17941 cttgctgtgg cgctgctgat tctcgccggt gtcgcgtggc ttctcttgcg cggtctccgc
    18001 ctctttctgc gccggtcccc gattcgtctg cctgtctccc tgcgccacgg catcgcaaac
    18061 gtgtaccgtc cgggcaatca cgctgccagc gtgctggtcg cgctggggat cggggtgatg
    18121 ttcacgctca ccatctatct ggtccagaag tcactgctga tcgaagttgc gggggctgcg
    18181 ccgcccgact cgccaaacgt cttcctcatc aacatcacac cgcgcgatca cgaagcagtc
    18241 acgcagtttc tcaacgcaag aagggacctt cgggcgaagc cgcgcatcgt gccgcttgtt
    18301 ccggtgcgcc tcctcctcgt caatggaaaa cccgtcgagt acatcgaaca cgcaggacgc
    18361 cactggcatt ttcgatacac gcgacaggtc acctggagcg aatcgaagcc cgacgacgtc
    18421 gaactgcgcc agggtgcgtg gtggacgggt ggtgagaaaa ctcccagtgt ttcggtgtct
    18481 gaagataccg ccgcggcctt gaaaatcgaa cctggcacga cgcttggatg gctttgcgtc
    18541 gaccaacaat tcgacgcccg agtgtcggcg attcatcgtg tgaagtcggt ccggccgggc
    18601 cagtccgacg acttcatatt caaccgcgtg gcactggacg ggtttcccgt gcaatggttc
    18661 ggtgccgtcc ggatggcgcc aaaccgcgta gcaggattgc agcgggaggc ctatcagcgc
    18721 tttcccacag tcaccgtgat caacgcagcc gacgtgctca acatcgtaca ggaggtggtc
    18781 gaccaagtgg cgctcgtcgt gcgattcatc tccgcgttcg caatcatcgc cggcgcgatc
    18841 attctggcat ccacggtggc cggcacacgc ctccgtcgca cgcgcgaagc agcggtgctg
    18901 aagactctgg gtgctcgccg ccggcgtctg gtggaaatct tttcggtgga attcgctatc
    18961 ttaggcgcgg tcgccggact catgggcggc gcactggcca cggtgttctc gcgccttcta
    19021 ttgatccgcc tgctcgacgc aaagttcagt ttcgacgtta ttccgaacct ggccacggtt
    19081 ctgctgacgg ccgctctggc gatcattacg ggctggcttg cctccgtacg catcctgcgc
    19141 gagcggccat tggaagtcct gcgccacgag tgagcgtggc tcaggagaat cgcgtgcgtt
    19201 tccagagcag ggttccgcag accaccagga acacgagcgt ggccgcggcg agaagaataa
    19261 tccagaacgg gttgacggcg atgggttgcc gttcagcgcc ggacgccaac cacacgaacc
    19321 ctccaagagc acacgccaca aagatcagct cccaccagcg gcgggagggg gtttccttgt
    19381 cgagcggatc atcgccctgc aggactgccg caaccctctc acggttcagc ccgtaacggt
    19441 cgcactcccg ttccatcgcc ttcctccagg atgcctgctc ggactccggc gcttcggcga
    19501 agctgatcga tgcggctcct gcgatcatca cgagtgagcc tgcgactacc agaatctggg
    19561 ctgtcaggcc gagcccggcc agttcgccga agacaagcgc tccccacgcg agcccccaga
    19621 gctggttcgt attcgagagg ggaatgccac gccctatgcc gatgtacttc gccgcgtact
    19681 gctgaaagag atccccaagc acccagcaga aaccgccgag gaagagccag aacattgcgg
    19741 atcgggcact gtcgagctcc acgagaaggc catggaatcc gccgtggaac accgccgcca
    19801 gcgtaatggc tgtgcccaat tcgccgaatg tgaacacggt gacgaacgac agcggattca
    19861 tgccgctgat gtaggccttc cggtagggga tgtacatcgt gccccacaac acgcccgcgg
    19921 ccaacgcggc gaggatgccg acggcggcac ggttcggcgc aaccgcggac tggtgtgacg
    19981 tggcccaggc cagcaggcac gctccggtca acatcgccgc gcctcccccg agaacctttg
    20041 cccattgctt cgcgccggcg ttgcgcagct cgttgaaaag cagccagccc cagaacaggc
    20101 cgatcaggct gtttgtgttc cacagcggga aggcgatcga gagtccgaca ttgcggatgg
    20161 cgaacactgt gagcgtgttg gcgaccgccc agagcatgcc ggccagtacg gcccagacga
    20221 ccaggtgaac tttctccctg aggtccagca gaacatagcc ggtccctttc aatagcaccg
    20281 gcaccgtcca ccgagccacg aagacgcccg ccaccattcc cacggagata acgaacgggg
    20341 agtagcccat ggtcacgagc tttgtcgggg cctcggcggc gccaagccag gcgcccgcag
    20401 cgaggccaca gatcacgccg agccggtgaa gcgaaagagc cgggggcagg gtcgcggatt
    20461 tcgccgtggg gctcatgcac tcaccgcaaa accatcagaa gcccggttcc taacacgagg
    20521 acgatcaccg gcacccagaa gtggctgtcg gtgaggattg atcgaacgcg cgagtgtttc
    20581 aagtgtgctg tcccggtgag aagcgttacg acatcagctt tcgaagatag ttgatgttgc
    20641 ggcggaggtc gttctccatc gagcctgagg gagtgttggt ctcgaggttt gcatagccct
    20701 cgaacttgat gtcggagacc gcctggagca ccgcgcggta atcgaccttc ccctcgccga
    20761 ggtagctctt gtccttgaag tgaaactggc agatgcggtc tttgccgagc cagcgtatct
    20821 ccttcggagc atctacgccg accatgttgg tggcgttacc cgcgtcgtag taaatcttga
    20881 acgcctttga ggccactcgg tccatcgcgc gcgcgttgtc ctcggcgttc agcaggttct
    20941 cgaaaccaag gatcacgccg gccttctcgg cctcgggcgc gagttccttg aagacgtcgg
    21001 cgacatagtc gagttctgcc cggttctcga cggaacactt tccgaagaac accgtcatca
    21061 ggatgctcgc gtggagcttc ttcgtgatgt cgataccgcg aagcacccag gtacgtgcaa
    21121 gcggatcgct cttcaggcag ttgacgtgaa gaacgtcgat gtaggtcgcg tcgatcggca
    21181 gcttgtgttt cttcgactcg gcgatgaatc gtccctgaac ttcctcgtct tcaagaggga
    21241 ggcgatcggc tccggccgtt ggacggccca acgtgacctg gagtccttcc atgccgaaac
    21301 tcttcgcaag cgcgatcgcc tccggcttgc cactctgtct caggacgccg tccatgacgc
    21361 caagtttgag gcctcggggc gcctttgccg cgagtggcgc tgcggcgacg gcaagaaact
    21421 gtctacggga aaacatgacc cacctcctcg gtctctctat ctctccggga cgagaacggc
    21481 gatgtccttg aaccaaacct cggtctccat gcggccgtga gcttgcaaag cgaggttgcc
    21541 ttccaaccgg ccctgcgtgt cattgggcag gtccaaggtc ttcacaccgt tgacgtggaa
    21601 aacgatccgg tgaccctgga gagaagcggt taactcgttc cagtcgttat ctttcacggc
    21661 cgcgttgtcc tccggtccag tcacccacgc ccggccgccc tggccggtct cccagaagcc
    21721 gccggtacgt ttcgtcgcgt cgatttcgac ctcgtaagcg gcgagggtct tcgggtcaca
    21781 ccgaacgaag tagccgctgt tgcctttcgg aatgcggaac atgatgcggg tggtgaggtc
    21841 cttgaacgac tggtcgctca cgatgaaacc gacgcgggtg tcgccagcga cactcaccgc
    21901 gtgaatggct ccgtcttcga tagtccagtt tccgccgccg cgtttcgtcc agccatcgaa
    21961 ggtcttgccg tcccagagcg gcttccatac gtgccggccg agatcttgga tgcggatgtt
    22021 gcgccagcgc acctgtgcgg gcttttcgcc cttataggaa tgaacctgga gtgcgatgac
    22081 tccggtcagg tccatggagt cgatgaggtc ggcgcaaggc actccgttca cccagacctt
    22141 gatggagtcg cccatggcgg cgacgcggta gcggttccat tggttgtcct taaacgcctt
    22201 acgggcgtcc gggtccgact cgatgttgtg cagccaagcg cggcgggctt catcgtaaat
    22261 cccgccggag gtgcctgact cctcgttcga gatttcaacc tggtagccgt agagccggcc
    22321 agggggcgtc ttatgctccg cgagcttgcc tttgtcgagc gcccaatagg tttggccttc
    22381 ggcgtagcgg tggctccgta tctggacgcc ggagttcaac gcgggatcag tcttggtctc
    22441 aaactcaagg acgaagtcgc cgtattcgcg ggtggtgcac aggaagctgt tggggctgcc
    22501 ctcggtcgta gtgccgacga tggcgccctt ttcgacacga taggtggcga ggccgttaca
    22561 gacttcccag cccgtgagcg attttccatc aaagaccggc acgaatttcg gatcagccgc
    22621 ggtggcgacg ccgcaaagaa cagcaaatag cgcaaggtaa cgtttcatgg atcctcagac
    22681 atagtaaatg atagccgctg tcaacggtcc cagcggcggg cagcgcggag ccgctcgatc
    22741 tcctcgcggg ccatctcctg aagcctcaga tagtccgtct tcactccgcg attcaatggc
    22801 atctggccag gctcgagaat cacgtagtgg cgcggtcgca tgtaagacgt catcgcacgg
    22861 gcgtgctctt tgatttcttt cgcggtcagg tccgcgccgg atttcttctc gacgaacgcg
    22921 acgatcgcct cggtgaagac ggcatgctcc atgccgacga cgccgcaggc cgcgatcttc
    22981 gaatgtgtgc aaagatggtt ctcgacctcg gccggaaaaa cctggtagcc gccgggcttg
    23041 atcacccact tggagcgacc cgcgaagtga aggcctttcc cgtcgcggaa ccccatgtcg
    23101 ccggtgtaga ggtggccatt ggcggagatc gcccttgcgg tggcggcagg atcattcacg
    23161 taaccgagga atgtctgcgg cccccgaaaa cagatctggc ccatttcgcc gtcgggcaac
    23221 tcgtcgcccg ccgagccgtc gtcccgcatg agctgccgga tcgacatcgg gtagaccggc
    23281 atctcgtggc cgatgctctc gatgatttcc gcgggactgg cgtcgaggtg cgtgtacgtg
    23341 cagaagccgg ctgcttcggt caggccgagg cccgtgccga tcttcggtga catcactgcg
    23401 agtctctcca ggaactgcat cggtacctgc tggccgccat agatgacgaa ttcgaggctc
    23461 gagagatcat acttgccgta gtccgaaagc cgccactcga actggtacat cgccggaatc
    23521 tggccgagta tattcacgcg atgtgtctgg atggcctcga ccgacctcag agcgtcgaag
    23581 acctcgagca gcactgccgt gcctccccag aacagggtcg tcatgagcgc ttcggcctgg
    23641 ccaccgacgt gggacggtgg gagatttacg agcagccgcg tgttctctcc gaacccgaag
    23701 gcagcgccga gacacatgtt ctgacaggtg atgttccggt gcgacagcag cgctcctttc
    23761 ggggtgccgg tggagccggt ggtgaagatg acctgcgcgc cgtcgttctc gtccacggcc
    23821 gccgtgactt tgttgaaagc cgcctgcagt tcgtccggtg gggcagccgc cgcgagttcg
    23881 gcgaaggaaa cggcgccttc gatagtctcc gccggatcgg tgagctgcac gagcgtccga
    23941 atcgtcgggc agtgggcctt ggccgcgcgc cccagttcgc cgaaatcgat tccacgagcc
    24001 gtgccgggaa acgcgtatcc cttcgctcca atcagattca aacagcggat cacctccgcg
    24061 ggcttcaggc gaaggtcgag cggtgcgtgg atgacgccga tcttgaagca ggcgtattcg
    24121 aggtagaggt gttccgtcag aaacggaaga gacgtggcga agaagtcgcc ccagcggaag
    24181 ccgagcccga cgagttgcat ggccacgagg tccgtgattc ggtcgaaggt ggcccagtcg
    24241 acttcctgtt tgcggtccgc gttgatgaac gcaaggctgt ccggctcttc tttcgcccag
    24301 tgcctgacga cgccgtggat caggtgacgc ccggcaaatt cctcctcgaa ccggttcagg
    24361 gtgcagttta acgaactcat ggcgcggtgt ttgattgtac cccggccgtt gttcggcgca
    24421 gggtaaattc aggaataatg gttcgtcggc gcgccggcgc ccggctcgac gccggagagg
    24481 gaggtagacc gtgatcatcg tccatgttca tgtccatgtg aaacccgaat tcattggggc
    24541 gttccggaaa gccaccgtcg agaacgcccg caacagcatt cgggaagcgg gtatcgcccg
    24601 gttcgacgtg atccagcgat cggacgaccc ggggcgcttc gtcctcgtcg aggtgtaccg
    24661 cacagctgag gcgcccgccc agcacaagga gaccgctcac tatcagacat ggcgtgacgc
    24721 ggtggagccg atgatggccg agccgcgtgg cagcgtgaag tacgccaacg tcttccccgc
    24781 cgacgagagt tggtaagaga gccatgcgtt tcgagttcgc cagcgcgggc cggatcgtct
    24841 tcggaccggg atctgtgcgc gaaatcgcac cggcggccag gaagatgggg cggtgcgtcc
    24901 tgctcgtcac cggcggttct cccgcgcggg cagccggaat cgcacgggaa ctcgaggcag
    24961 ccggcgtatc atgcaccggc tttcgcgtgg ccagtgagcc gacggttgaa ttaatccgcc
    25021 agggaaccgc ccgcgcacac gaagagcggt gcgacacgtt gatcgcggtc ggcggcggca
    25081 gtgtcatcga cgcgggcaag gcgatcgcgg cgctcctcgc caatggcggc gatccgctcg
    25141 actacctgga ggtgatcgga gcgggccggc ctctcctacg ggcgtcggcg ccgttcatcg
    25201 ccgttccgac gacggcgggg acgggctcgg aggtgacgcg gaacgctgtg ctcgcttcgc
    25261 tggaacaccg ggtgaaggcg agccttcgaa gcgccgggat gctgcctcgg ctcgcggtgg
    25321 tcgatccgga actgactctt gatttgccgc cggcgctaac ggccagcact ggcctcgatg
    25381 cactgacgca gcttatcgag ccatttgtgt cggtccgagc caacccgatg accgacgcca
    25441 tctgtctcga agggatgcag cgggcgtgcg gcgcgctcgc gcgagcatac cggaacggga
    25501 aggatagtga agcccggacc gacatgtcgt tggcgagcct gttcggcggc attgcactgg
    25561 ccaacgcggg actgggggcc gttcacggat tcgccgcacc ggtcggcggg atgttcgacg
    25621 cgccgcacgg cgcggtctgc gcggcgctgc tgccgcatgc catgagggtg aacatccgcg
    25681 cgttgcgtgc ccgcgcaccg gagagtgaag cgctgcggag gtacgagacc gttgctcgcg
    25741 tgctgacagg cgacgcggcc gcttcgccgg aggacggagt tgcttcgttg gagcggctgt
    25801 gtcaggagct ttccgtctct ccgctacgaa cgtacgggat cggtgacagg gatgtccccg
    25861 agttggtgca aaaggcggcg aaggcgagca gcatgaaagg aaaccccatc accctctccg
    25921 gcgacgagct gacagaactg ctggtgaggg cgctgtagcg gaaagtcatg catcggtccc
    25981 agccaggcgg cagcggcatg aagcggctag cgtgggtctg cgtggtcgtg tcggccgcgg
    26041 cgggcatcct gcccgggcag cgaaacaagg atgcagggct ggcgcgtgag aagctcccga
    26101 gctggctaaa gatcggcggc gaaatccgcg gccggttgga ggcggagcgc ggattcaagt
    26161 acgtgccggg tgaggacgac acgtactact tgcatcgcct cacattcgac atgagcgtcc
    26221 acccagcctc ctggctgtca ttctcggccc aggtgcaaga cgcccgggtc gcgggctata
    26281 gccgaggccc gtcgccctcc agcctggcga atccgttcga cctgcggtca ggcttcgtcg
    26341 agttaggccg tgacgcggcc aaggggtgga gtctccgcgc cgggcggcag gaaatgaagt
    26401 tcggagagga ccggctgatc ggtaactcga actggagcaa catccctcgc cggtttgacg
    26461 ctgtgcgtct cacgtggaaa cgtcagaatg cgacactcga ctggttcgcg tcatccgtgg
    26521 tcactacggt aagagacggc ttcgaccgcc cacggctcta caacggtttc ttcggattct
    26581 actcgtcatt ccggaacctt ccgggcaagt ccacggtgga accctatctc ctttggaaga
    26641 cgaacgctcg cgttcgcggc gagctcggca cgttcggaga tctggatgtg tacactgtcg
    26701 gcgcccgcgt ggtgggcgaa ctgccattgc gtttcgacta cggtgcggag gttgcgttgc
    26761 aacggggaag ctacgcggcc gacgacattc gtgcatgggc cggtcactgg cgtctcgggc
    26821 acaagttgcc gtacgcgaac aaggcgttcc ggctggtact cgaattcaat cacgcctcgg
    26881 gcgatggcaa accgcgggac ggacggcacg gcacgttcga tcagttgttc ccgaccaacc
    26941 atcagaagta cggaatcgcc gaccgtatta cctggcggaa cataaacgac ttgatggcgg
    27001 gagtcgagtg gcggccggcg aagaagtggc gttggctgat cgattaccac tcgttctggc
    27061 tggcgactct ccaggacggg atgtacaccg acgccggggc gctcgtggtt cgtaatccgg
    27121 gcgcatccag ccgccggatt ggcggcgagg tcgacgtgca gggatcgtac cagttcacag
    27181 agcatctcca gttcggcgcc ggattcggcc atttgtttgc cggccccttc cttcgggaat
    27241 cgattcccga aaggagttcc agtcacccgt acgtgatgtg gacgtacgcg ttctaagaac
    27301 cccgcttccc tgcgagcccg agccggccga agaacaattt gcgacactga agagagggcg
    27361 attccacaag caggtgattt atgagtctct tcttttccac gggatcggaa actgctgagc
    27421 tatcctccgc cgacctccgt aacggcttgt ttcaggcgct cgacgctctt ggaaagcgcc
    27481 gcaaagtgct atgcctgccg ccggatttta cccgctatca ctcccgggcc ggagaactca
    27541 cgaacctggc gtggcagtac ttcggagagc ggctgacgga tgtgctgcca gcgatcggga
    27601 cccacttccc ggtgagcgag cacgagatcg ggctaatgtt cggcgacatg ccgcgggatc
    27661 ggttccgggt ccacgacttt cgttgcggcg tcgtcacgct cggcgaagta ccgcgggagt
    27721 ttatccgcga gcagtcggag ggcaagctcg acttcacctg gccggcgcag gttgaccggc
    27781 ttctgatcga aggcggtttt gacttgatcc tctccgtcgg gcaggtagtg ccccacgaag
    27841 tgatcggcat ggccaactac aacaagaacg tcttcatcgg aaccggcggc gccgacggca
    27901 tcaataagag ccactacctc ggcgctgttt acgggatgga gcggatcatg gggcgcactg
    27961 atacccccgt ccgccgggtt ctcaattacg cttcggagca tttcgcaggc aatctgccga
    28021 tcgtgtacgt gctgaccgtc gttggaaaga acgaggccgg cgatctggtg acccgtggat
    28081 tgtttgtcgg cgacggccac gaatgcttcg agaaggcggc tgcgctcagc ctcaaggtca
    28141 actttcagat gctcgaccgg gagatccgca aagcggtggt ctacctcgat ccgggcgaat
    28201 tcaagagcac atggctgggc aataaagcgg tctaccggac acggatggcg ctggcggacg
    28261 acgccgagct gatcgtgctc gcgccgggcg ttaaagaatt cggcgaagac ccggccatcg
    28321 atacgctcat ccgcaagtat ggctacgtga agacgccgga agtccttcag gcagtggaga
    28381 agaacgcaga tcttgccggc aatctcagtg cggccgccca cctgattcac ggctcctccg
    28441 aagggcggtt cacgatcacc tactgccccg gcggcctcac gaaagaagag accgagggcg
    28501 tcaatttccg ctacgcaccg ctggatcgga tggcacggtg ctatgccccg gaacgactga
    28561 cagacggctg gaacctggtc gatagcgagg aggtcttcta catctccaac ccggccctgg
    28621 gcctctgggc ctaccgcggc cggtttgagc agtctgcgta gcgcggctta ttgccggcgc
    28681 ctcccggcca tccggcgagc accccacccg aaggcccgac taccattccg ccccctggga
    28741 agcgtcgcac ggcgccggcg aaggccacct cacccgcgta gttgcatgcc gctttttcct
    28801 ctgcttcaag gatacgtgaa acactttcac accgtgccgc agaggtataa gatcgctgat
    28861 gcggacgtta ttgcagtcgg gagtaagcca atgcggacac tgacagtcgg tttgatcttc
    28921 tttgcaacga tcttccttgc cgccccttcc gctctcggcg cgggccgcgc caccctcatc
    28981 agggaagggg agataggccc gccagccgaa gacgggttgg tccggctcga acaagcgtta
    29041 cagaccaagg gcatcgtagt cgatcggtcc gatggctcgc aggccgtcta tggtgacgtc
    29101 gtagtgctcg ccggactcgg ctccggcgcg gtggccgccg ctctaaatgc tgaggacgtc
    29161 actctcccgc agggtcccga agctctcgcc atccggagaa cgacgcacca ggggaagcca
    29221 gcagtcctac tgtggggatc ggatacccgg ggcctcatgt acgcggccct cgagacggcg
    29281 gaccgaatcc tttgggcgca gagtgcctcc aatcccttcg ctcaaattcg cgatgtcgtg
    29341 gaagagcccg atgtcatcga acgctccgtc tcgatctaca cgatgcagcg cgcgtacttc
    29401 gagagccgtt tgtacgacga aaggtattgg cggcggtact tcgacatgct gtccgccagc
    29461 cgtctcaaca gcttcgttgt gatcttcggt tacgagaacg gcggcttcat ggccccgccg
    29521 tacccgtact ttttcgatct cgacggcttc cccggcgtgc ggctcaccgg aatcacaccg
    29581 gcgcagcagc agcgtaacgt ggctgcgttc aaagcaatgg tccgcatcgc ccacgaacac
    29641 ggtatcgacg tgatcgccgc catctgggac cacatctacc gcggcggtgt ccagggcggt
    29701 ggcatcccag gcgcctccga aaacgccggg aaggaggcgc cccaccttgt gtccggtgtc
    29761 actgcggaga acctggttcc gtacaccaag gcggcgctgc tccgtttcct ggaggtcttc
    29821 ccggaacttg acgggttgca gttccgcatg cacggtgagt caggcttgaa gcgggaggag
    29881 atggagagct tctggcacga cgtcttcggc atcatccacg aaaagaaccc cggcatccgg
    29941 gtcgatctcc gcgctaagga attgcccgac tcgatcatca acgatgcgct gggccagggc
    30001 ctaaaagtgc gtgtgaccac aaaatactgg atggagcaga tgggcatgcc cttccatccg
    30061 acgcacatca accgccagaa ccaacgcgac cgccgtcatg gctacgccga tctactccgc
    30121 tacccgcaga aatacacggt ccattggcgt ttgtggaacg gcggcacaac gcgccttttg
    30181 ctctggggcg acccggagta cgtcaagcga tttgcctcca caacacacgt ttacgggggc
    30241 cgcagcttcg agatcaacga gatgctcgcg accaagatgc tcggatctcc gcataacgag
    30301 ccgccggccc cgattcttaa ccagtcctac cgctactacg actacgaatt cgagcgttac
    30361 tggcacttct atcaggtctg gggacgggtc agctacaacc cggatacgcc tcccgaaacc
    30421 tgggaacggg aatttcaacg acggtttggg cccgtcgccg gcgcgcaggt gatggacgct
    30481 ctccacctcg ccagcaacgt gctcccgcgc attgtcgccg ccagttacct ctaccagcac
    30541 tttccgacga cgcgcggctg ggccgaaatg atgcgcgggg acgaccttcc gaagtatgcc
    30601 ggcatggagg gaagcgacat cgcccaattc atgaatcttc gcgatgcagc ccgaagcctt
    30661 atcgagggta cggaaaccgc gatgcggcgt ccggaggaaa ccagccgctg gtttgaaaac
    30721 gtctccgaac gaatcacggc tcatgtccgc gaggcggaga cagccgtggg cgggcgcgcc
    30781 ggcaaggaat tgatctccac tcttgtggat ctgaagatcc ttgccggtct ggcaaaatac
    30841 catgccgtgc gcctgcgggc cggcctcagt tacaacctgt acaaggaaac cggcgatctc
    30901 ttttccttcg acgatgctgt cgcgcaggag aagcaggccg tcgcagcttg ggagcgaatc
    30961 gtcgaagccg ccggaggtgt ctacagtgag aacctcgcct tcggggtgca cgaggtgggc
    31021 tttagccgtc attggaaaga agaattacag gcattgcgcc gcggcctcga tcaactcctc
    31081 gccgagcgtc agcaaagccg acttcaggga gcgagtctga aatcgcgtat cgcgcacgtg
    31141 cctgtccggc agtccggtcc cggaagcgcg atccgcattc gcgccacggt cgccttgccc
    31201 gaaccgaaac aggtgaacgt actcgtcgcc ggctcgaaag gagggttccg tgcggtccct
    31261 atgcagcgag tcgaagccgg aatgtaccag gcggaggtca gaccgggttc cgccgacgac
    31321 cgtctccgct atttcatcga ggtgcgggaa gcgtccggcg gtccctccac atggccgcag
    31381 aagggcaaga atgatccggt ggtcgtcgcc gtcaccgcgg accgccagcc gccttcagtc
    31441 tggccagacc ctccggcgaa agcgaagcca ggcgagaacc tgaccgttac ggcacgggta
    31501 caggatccgt cgggcgttgg atcggcgcgc ctccggtaca ggcacctgac gcagtttgaa
    31561 gactaccatc tcagggaaat gacgctcgat ccgaagaccg gtctgtacac cgcacagatc
    31621 ccgggctcct ttatcactcc tgaatgggac ttgatctatt tcatcgaggc gctcgacatc
    31681 catggcaacg gccgcatgta tcccgatctc gagacggata ccccgtatat catcgtgagt
    31741 gtcgcgcggt gatcgtggcc tggtgggaat tcgtacgatg agggaagcgc ccgggggcgc
    31801 gcctgcgcaa ggagccgcgt ctgcctcgct acattcgaac tttgcctgga ccttcgcggg
    31861 gaattcgttc tacgccgccg gccagtgggt gattctcagc ctcttcgcga aactgggcgg
    31921 cagtgagatg ctcggacagt acgcgctcgc ggtcgcgttg acggcgccgg tcgtcatgct
    31981 gtcgcacttg aacctccgag cggttctcgc gacagacgtc ggtgggcagt atccctttgg
    32041 ggattacctc gctgtacggc tcggcactac ggcggccgcc ttggtcgcca tcgccggcat
    32101 cacggcggcg gctggcaact cctggccgct cgctgggtcc gtggtggcgg tgggagtcgc
    32161 gcaatgcgcc gagacggtca gcgacatcta tcacggagcc atgcagcgcc gcgagcggat
    32221 ggaacagatc gcccggtcga tcatcgcacg cacgagcgtg tc
//
LOCUS       DATIFQ010000338         4928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1262431, whole genome shotgun sequence.
ACCESSION   DATIFQ010000338 DATIFQ010000000
VERSION     DATIFQ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262431"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VLE22_15675"
     CDS             <1..231
                     /locus_tag="VLE22_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15897.1"
                     /translation="MTVSNSAAGSVIAARKIIISVRMIRTKLASELTNSPRQLYSQDF
                     LFRILGTIDSTPSSGISASAICAPTRSGRTTT"
     gene            231..521
                     /locus_tag="VLE22_15680"
     CDS             231..521
                     /locus_tag="VLE22_15680"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSB15898.1"
                     /translation="MFLSAICHMLSPEENRDLFKRCYRALASRGRIVIRDFFLEPDKT
                     APKWVALFALNMLVGTQGGGCYTKEEYTAWLRLAGCQEVEILGSSGMITGRR"
     gene            complement(957..1829)
                     /locus_tag="VLE22_15685"
     CDS             complement(957..1829)
                     /locus_tag="VLE22_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15899.1"
                     /translation="MNMMFRQAGVALAMVVVNLIFCLHAQIQSMQRSFYAEQRLEVWY
                     EDGRRDEDVIRITRLANRSFLAMHPLHSVKDRMRHIGGYYFYLVDTEALVEYSGETTL
                     RAAWPLYMDSAVPGQNKMVDQYGDCSVLADDSIKKVPAGKIRRFAVLRIERVEGRERY
                     TTWIAPELGCFPLRSQVLVDELVRERVETVRLDLLPAQTRLSIPKGVRIVSPKKYCDI
                     YQKRYGQEYLPQRVCDKYEKIYQEVKAAKRQRSSPRAGFANAADHGATGYACRRSSRV
                     GSARLTPSTRRDDC"
     gene            complement(1985..3055)
                     /locus_tag="VLE22_15690"
     CDS             complement(1985..3055)
                     /locus_tag="VLE22_15690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15900.1"
                     /translation="MRRRSLMHAVASGCSILLLAVLPSAGQSTHAIPVFSTEGIGAVG
                     RSVKVLAPGMVLMLYGGHLASEPVCGQPKVQPALELCGVRVPIGNSRAELLYVSSGQI
                     NFKIPADVPAEGFAPLRVCVGAACSTPVRMWFSTRTALLSLERPAYVHMPVWIHVDPP
                     PPYFVSYPCWNGPWILSGYEFEVRRNGRSLAPMPQRSPPPNWAGSSAERCDGPTVGSS
                     LPLHLLYRFDEPGTYSVRFTAKKGDQILYRSDWTDIVVGPFSEEKRDAWLRSLDIEIK
                     MNSRSVVSHMIPSLLAWPDEKALAVLLKVIPANTMQCTNFDCIKLGLARASLAWFDDA
                     LLRSQIRPERLLRLCPHEGKCK"
     gene            3356..3874
                     /locus_tag="VLE22_15695"
     CDS             3356..3874
                     /locus_tag="VLE22_15695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HSB15901.1"
                     /translation="MLDPPVGSSLLIRRASPMDAAGIVAVLEVIAAERIHSVIDQAWT
                     IDQEGRYLESLSAREAFHVAVDAARGIVGFQSLDLWSPLLNSMAHVGQVGTFLLPEWR
                     GLGLGRQLWRATEFFARDAGYRKLVIQVRASNTPAQAYYRCLGFQECGRLTRQVVIDG
                     VEDDEVLMEFFL"
     gene            4273..>4928
                     /locus_tag="VLE22_15700"
     CDS             4273..>4928
                     /locus_tag="VLE22_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15902.1"
                     /translation="MLRFLPLFLAAACSVAQPQRGPIAAIEFYGAAAVDYTRLRAAFP
                     FQVGDIFELKEATFQDRSPEFQKLISRNRISVAPVFIPDLNGWVLYVDVEPPDTSPAF
                     WNPAPAETEKLPAKIVRLYEHAMDRYVNGGIHAGDDTTNGYSLSKDPIMRSDELKLIE
                     YARAHAKRIYSILKRSASPQDRIAAAWIAGYAPKGLDQLTALLHSVRDPDSTVRNNAI
                     R"
BASE COUNT         1041 a         1496 c         1409 g          982 t
ORIGIN      
        1 atgactgtgt ccaactccgc cgcgggaagc gtaatcgccg ccaggaagat cattatcagt
       61 gtgcgcatga tcagaaccaa gcttgcctcg gaacttacca actcaccacg gcaattgtat
      121 agccaggatt tcctttttcg catcttgggc acgatcgatt ccacacccag cagcggaatc
      181 tccgcgtcgg cgatctgcgc accgacacgt tcgggcagga ctacaacctg atcttcctat
      241 ccgcgatctg ccacatgctg agcccggagg agaacaggga ccttttcaag cgatgttacc
      301 gggcgctggc gtcgcgcggt cgcatcgtga tcagggattt cttccttgaa ccggacaaga
      361 cagcgccgaa gtgggtcgcc ctctttgccc taaatatgct ggtcggaacc caagggggtg
      421 gctgttacac caaagaggag tacaccgctt ggctccggct ggcaggctgt caggaggtcg
      481 aaatcctggg ctcgtccggt atgatcaccg gcagacgttg acggcacgaa ccgataaccg
      541 gaagccctgg gtgggcagga ccagagaagc tctggccttg gcggcggttc tgacagagcc
      601 cgaaagacgg cgatgccgag cgtccgcccc tgggagcagt tgaggagaat cggcttatcg
      661 cggctccccg acccgattcc gcgaacataa gtccacaccc gatcggcggt cgaaaaaatt
      721 gttggcatcc gtagctgctg gagcccgctc gcctgctgct gccaggaggt ctcggagctt
      781 agaagcgctg tcgaactact tggaagagct tcgtttctac cgacgggacg aacgaggcca
      841 gattgtaacg gggtgcgacc acctgcagaa cgccacacgg tccctggtgg taagcggaat
      901 gtcgcacatg cgtaccgagc cagtgaggac accgcccgag cactggtacg tagatgtcag
      961 cagtcatccc ttcgggtgga tggcgtaagt cgagcgcttc cgaccctgga ggaccgacga
     1021 cacgcatagc ctgttgcccc gtgatccgca gcgttagcaa agccggctcg aggtgaagaa
     1081 cgctgtcgtt ttgccgcctt gacctcctgg taaatcttct cgtatttatc gcagacccgt
     1141 tgcggcaaat actcctggcc gtagcgtttc tgataaatat cacagtactt cttcggcgaa
     1201 acgattcgaa ctccctttgg aatcgaaagc ctggtttgcg ctggcagcag gtcgagacgc
     1261 acggtttcca cccgctcccg aaccaattcg tccaccaaca cctgggaccg aagcggaaaa
     1321 cacccgagct ctggagcaat ccaggtggta taccgctccc ggccctctac cctctcgatg
     1381 cgcaagaccg cgaaacgcct gatcttccca gccgggacct ttttgatgga gtcatcggcc
     1441 aaaacggagc aatcgccgta ctgatccacc atcttgttct gaccaggcac agctgaatcc
     1501 atgtacagag gccacgctgc ccgaagggtg gtctctccgc tgtattcaac taacgcttct
     1561 gtgtcaacca agtaaaaata gtacccgcct atgtggcgca tgcgatcctt cacggaatgc
     1621 agagggtgca ttgccagaaa gcttcgatta gccagacggg taatccggat gacgtcctca
     1681 tcgcgccgcc catcttcata ccaaacctct agtcgctgtt ctgcatagaa tgagcgctgc
     1741 atcgactgga tctgcgcgtg gaggcaaaat atcagattta caactaccat cgcgagagcg
     1801 acgccggcct gtcgaaacat catgttcaca gacccactgt catccaccgc cccagcagaa
     1861 cgcaactacc tcccatttcg cgaacataag tccacagtcc cggatcaacc gctccgagcg
     1921 gttcaacggc tccctgcgct gtcggatagt ttccggtaac cccacttatc tgccccaagc
     1981 ccgctcactt gcactttcct tcatgcgggc ataagcggag cagcctttct ggacggatct
     2041 ggctgcgtaa caaggcgtca tcaaaccacg cgagggaggc cctggctaag ccgagtttta
     2101 tgcaatcgaa gttcgtgcac tgcatggtat tggcaggtat gactttcagc aggacggcaa
     2161 gcgccttttc gtccggccac gccagcagcg agggaatcat gtgagacacg acgctgcggc
     2221 tattcatctt gatctctatg tccagggacc ggagccacgc atcacgcttc tcttccgaga
     2281 acggtccaac gacgatgtcg gtccagtccg accggtagag aatttggtct ccctttttgg
     2341 ccgtgaatcg cacagagtac gttcccggct catcgaaccg gtacaaaagg tggagcggga
     2401 gcgaacttcc gaccgttgga ccgtcgcacc gctcagcact gcttccggcc cagttcggag
     2461 gcggcgatcg ttgtggcatc ggagcgaggg acctgccgtt tcggcgcact tcgaattcat
     2521 agcccgacag aatccacggt ccgttccagc acggatagga cacaaagtat ggaggtgggg
     2581 ggtccacatg gatccaaacc ggcatgtgaa cgtaggctgg cctttccagg gagagcaacg
     2641 ccgtgcgagt gctgaaccac atgcggaccg gcgtgctaca cgccgcgcca acgcacaccc
     2701 gcaacggcgc gaatccctcc gcgggcacat ccgccgggat cttgaagtta atctggccgg
     2761 aactgacgta gagcaactcc gcgcggctat ttcctatcgg gacgcgaacc ccgcacaatt
     2821 ccagagcagg ttgcactttc ggctggccgc agaccggctc ggaagccaag tgcccgccgt
     2881 agagcatcag caccattcct ggagccaaaa ccttcacact tcggccgacc gcgccaatac
     2941 cctcggtgct gaagaccggg atcgcatggg ttgactggcc tgcggagggc aacacagcca
     3001 gcagcagaat agaacagccg ctggcgacgg cgtgcatcag actgcgtctc cgcatcacct
     3061 ctatatttgc accgagcaat cgactgaata gacaccgagt gattcagtgg agttcctgcg
     3121 cgggttgccg cattgtgggc gatacacggc gttaccagaa acttgcccca gacggcgttt
     3181 gcgccagatt ccggtcggat cgtcgggaag cccggaagcg acgaacccga tacgtcgcaa
     3241 agtacggatg gcgcgtcagc cgaccggaat gtccatgtcg tgtgcgacgg atcatcggta
     3301 aacgctggcg acaagtccgg agctaccggg aagcaggctc tggcatactt ggacggtgct
     3361 agacccaccg gtaggatctt ctctgctgat aaggcgagcc tcgccgatgg acgccgccgg
     3421 aatcgtcgca gtgcttgagg tgatcgctgc tgagcggatc cattctgtga ttgatcaagc
     3481 gtggaccatt gaccaggagg ggcgctacct ggagtcgctc tctgcgcgcg aagcgtttca
     3541 tgtagcagtc gatgcggcgc gcggaatcgt tggttttcag agcctcgacc tgtggtcgcc
     3601 gctgctaaac tcgatggctc atgtcggtca agtaggaaca ttccttttgc cggagtggcg
     3661 agggctgggc ttgggtcggc aactgtggag ggctactgaa ttctttgctc gtgatgcagg
     3721 ttatcggaaa ctggtcatcc aagttcgcgc atcgaacact cctgcgcagg cgtattaccg
     3781 atgtctgggg ttccaggaat gcggccggct aacccgtcaa gtggtgatcg acggcgttga
     3841 ggacgacgaa gtgctgatgg agttcttttt gtaggggcga gcgctctcgc acgtgttaac
     3901 gacgctcaga gatcctctgt gacgccgcag tgtagatcta aatcgctcga ttcacagatg
     3961 gtcggccgct cggagaccat cggccacggc cttcagccac agcccttcag ccacacgatc
     4021 ccaaagtcgg caggccgtag atgggggcca cacccgatcg ggggtcgaaa aaattgttga
     4081 tgcgtgtggg aagcgcgccc tcgtgcgccg ctcgttgaac gcctgctgcg cccgatgctt
     4141 cgccccgcga gaacctccac cacccgctcc cggggtgccg cctcctcccg ggcgcgctgg
     4201 ggtaagagaa aagtgagttt tcggcagtcg tgcgcgccgc cgccttcggc ccccacattc
     4261 tataatcagg cggtgcttcg gttcttgccg ctcttcctcg ccgccgcctg ttcggtcgca
     4321 caaccgcagc gcggccctat tgcggccatc gagttctatg gagccgccgc cgtcgattac
     4381 acacgtctcc gcgccgcttt tccgttccaa gtcggcgata tctttgagct caaagaggca
     4441 acatttcagg atcggtcgcc agagtttcaa aaactcatta gcaggaaccg catctcggta
     4501 gcgccggttt tcattcccga cctcaacggc tgggttctct acgttgatgt agagccgccg
     4561 gacacatcgc cggcattctg gaatcccgcg ccggcggaaa ccgaaaaact gcctgcaaaa
     4621 attgttcggc tgtacgagca cgcaatggac cgatatgtca acggagggat ccacgccggg
     4681 gacgatacga cgaacggcta ctcgctctcc aaggatccaa tcatgcgttc agacgaactc
     4741 aagctgatcg agtacgcacg agcgcacgcg aagcgcatct actcgatctt gaaacggtcc
     4801 gcctcacccc aggatcgaat tgcggcggcg tggatcgctg gctacgctcc caagggtttg
     4861 gatcagctca cagcgcttct ccactccgtt agggatcccg attcgacagt ccgcaacaac
     4921 gcgatccg
//
LOCUS       DATIFQ010000339         4015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1262745, whole genome shotgun sequence.
ACCESSION   DATIFQ010000339 DATIFQ010000000
VERSION     DATIFQ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262745"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(678..753)
                     /locus_tag="VLE22_15705"
     tRNA            complement(678..753)
                     /locus_tag="VLE22_15705"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(718..720),aa:Phe,seq:gaa)
     gene            complement(821..2338)
                     /locus_tag="VLE22_15710"
     CDS             complement(821..2338)
                     /locus_tag="VLE22_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HSB15903.1"
                     /translation="MQFLTGSLLELITQTSTNLPPDVRAAMGVAMRREESGTQASQAL
                     EIIAGNIDMAQDDEAPICQDTGMPTFVIHTPIGVNQLQIRKAVQEALAEATRLGKLRP
                     NSVDSITGKNSGNNLGEETPVVHFEQWEEDEIEVKLILKGGGCENKNIQYSVPCELDH
                     LGRADRNLEGVRKCILHAVWQAQGHGCAPGAIGVCIGSDRAHGYTLAKYQLFRPLDDE
                     NPNPKLAALETRIMDEANRIGVGAMGFGGKASLIGCKITAANRLPASFFVSVAYDCWA
                     FRRLGIRVDPASGAITRWLYRDPDCPVERMAKAEGFPLTGREIVLNTPLTEEQVRALK
                     VGDVVLINGEMYTGRDQVHAYLMKNAPPVDLNGAIIYHCGPVMLKQGDGWVPKAAGPT
                     TSSREEPYQAHTIRTYGVRGVIGKGGMGAKTLSAMKDTGAVYFHAIGGAAQVYARAIE
                     KVVGVDLMEFGIPEAMWRLRVKNFLCIVTMDAHGNSLHADVERTTGAALSELQAV"
     gene            complement(2357..3352)
                     /locus_tag="VLE22_15715"
     CDS             complement(2357..3352)
                     /locus_tag="VLE22_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15904.1"
                     /translation="MRGKWLLLSATLILIAVAVGALSLLRRESGPAPPKKTAAAAPPA
                     FAGTEASLRGRIQARKIVNVPVPVDGAVESFLADVGQSVYEGQLLAQIANTALESERD
                     AALTELQRAQARVSSLESRLIAARLDSSRARADASRARNDADKSEKTYLRQQLLFREG
                     AAARVSFEKSQQQFENDKTEFEHLDEMARLSEQNVDAQIRELDEAKRSQSEKSRQYEE
                     VGDAIASAQVVSPVDGLIVGRRGQAGQDVTIEMEDLFQIAVDLSELDAVLEPEPQVLQ
                     RIRPGQEAVVQVAELLSSIPGKVREIKESRVFVEFTSPDPAVKPGMTVQVRIKLT"
     gene            3420..>4015
                     /locus_tag="VLE22_15720"
     CDS             3420..>4015
                     /locus_tag="VLE22_15720"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HSB15905.1"
                     /translation="MSLPQAAAEFIAAQVERLRRSVKRKRIVFPESSDPRILAAAGRL
                     AQEGLLEPVLIGPAPSGAPANVTFVVPESSPKLRSYAAIYFDRRRARGVTEAEAMEIA
                     RTPLYFAKLMVAAGDADGSIGGASISSAEAVRAALQCIGVPPHIRTVSSFYIMALHDR
                     AFGHKGLLFFADCSIVIDPSAVQLADIAIATAGSVRNLL"
BASE COUNT          679 a         1318 c         1211 g          807 t
ORIGIN      
        1 tctttcaccc tttcgaaggc tagccgatag tccggatcct ccttcagcca gtggtaatgt
       61 cgccgccgtc cgatctgtgc ggcttgggcg gccttcctga cgcaggctgt ggttcgaaag
      121 gcggccaaga acgctgcttg cctgaaaacg caccgccgcc tcggacgcgg tgaataccct
      181 attggcgcgc tgcccgtggt ttcctggcct cgcaaagccc cttggccctg ctctgagtct
      241 acccttctgt ctcgtctcat acctgagtaa gtgcgttctc ttgccgacta cgtttcgtcg
      301 tctccctgtc cgaagatcca aatccaggac cctgtcctac tggtggtgga agcgagtgcg
      361 ccacagttcg atcgctgcca ggattttgac gctcttgcgt tctcgcgagg aacgccgccg
      421 ccacccgacg cttcaccaga ctgccgccgc gccaccggca gaagcctctt cgcggtccac
      481 cttctcgtgt aggtgcgaac caggggggcg cggaagccgt cgtcgactcc gcgctacccc
      541 tggagtgtga tgagggcgca ctacaaatcc atccccatac cctgattcac cccgcaggcg
      601 aatcccggcg gaccgaaagt tttggaatga accggaatga aactgggttt tggaaggtct
      661 gtaagtgatt gaaaacatgg tggccaggga cggaatcgaa ccgccgacgc cagccttttc
      721 agggctgcgc tctaccaact gagctacctg gccggagatc cttattgtag cagatgctcc
      781 gcgggacagc tctcacgcta ctcatcacgc ggcccgggat tcaaacagcc tgcaattcgc
      841 tgagcgcggc gccggtggtg cgctcaacat cggcgtgcag gctattgccg tgcgcgtcca
      901 tcgtcacgat gcagaggaag ttcttcaccc ggagacgcca catggcctcg ggaatcccga
      961 actccatcag gtctacaccg accaccttct cgatcgccct cgcatagacc tgcgccgcgc
     1021 cgccgatcgc gtggaagtag acggcgccgg tgtccttcat cgccgagagc gtctttgcgc
     1081 ccatgccgcc cttgccgatc accccgcgca cgccatacgt gcgaatcgtg tgcgcctggt
     1141 atggctcctc gcgggagctc gtggtcggtc cggctgcctt cggcacccaa ccatcgccct
     1201 gcttcagcat caccggaccg cagtggtaaa tgatcgcccc gttcaggtcc accggaggag
     1261 cgttcttcat cagataggca tgcacctggt cgcgacctgt gtacatctcg ccgttgatca
     1321 acaccacgtc gccaaccttc aacgcgcgga cctgctcctc ggtgagcggc gtattcagga
     1381 cgatctcccg gccggtgagc gggaatcctt cggccttcgc catccgctcc accgggcagt
     1441 cgggatcgcg atagagccag cgggtgatcg ccccacttgc cggatcgacg cggatgccca
     1501 gccggcggaa cgcccagcag tcgtaagcaa ccgacacgaa gaagctcgcc ggaaggcggt
     1561 tggcggcggt gatcttacag ccgatcagcg atgcctttcc accgaacccc atcgcgccaa
     1621 caccgatccg gttcgcttcg tccatgattc gagtttcaag cgccgcgagt ttcggattcg
     1681 ggttctcgtc gtcgagcggc cggaatagct gatacttcgc cagtgtgtaa ccgtgggccc
     1741 ggtcgctgcc gatgcagacg ccgatcgcgc ccggcgcgca gccatggcct tgcgcctgcc
     1801 ataccgcatg gaggatgcac ttacgcacgc cttcgagatt ccggtccgca cggccgagat
     1861 gatcgagttc gcaaggcacg gaatactgga tgttcttgtt ctcacagccg ccgcccttga
     1921 ggatcagctt gacttcgatc tcatcctctt cccactgctc gaagtgcacg accggcgttt
     1981 cctcaccgag gttgttaccg ctgttcttac cggtgattga gtctaccgag ttgggccgca
     2041 atttgcctag cctggtcgcc tcggcgagcg cctcctgcac ggctttgcgg atctgcagtt
     2101 gattgacgcc gatcggagta tgaataacaa aggtcggcat cccagtgtcc tggcagatcg
     2161 gcgcctcgtc gtcctgggcc atgtcgatat tgcccgcgat gatctccaac gcctgcgaag
     2221 cctgggtacc agattcttcc cggcgcatgg caacacccat cgcggcgcga acgtccggtg
     2281 gcaggttcgt tgaggtttgg gtgatcagtt ccaggagact gccggtgaga aactgcatgg
     2341 gattcctcca aaatcgttat gttaacttga tgcgcacctg caccgtcatc cccggcttca
     2401 ccgccgggtc ggggctggtg aattcgacga acacgcggct ttccttgatc tcccgcacct
     2461 tgcccgggat actggagaga agttcggcga cttggacgac cgcttcctgt ccgggccgga
     2521 tgcgctgcaa cacctgaggt tcgggctcaa ggacagcgtc cagttccgag aggtccacgg
     2581 cgatctgaaa gaggtcttcc atctcgatgg tcacgtcctg acccgcctga ccccggcgcc
     2641 ccacgatgag gccgtcgaca ggggatacta cctgggcgga ggcgatcgcg tcaccgactt
     2701 cctcgtattg ccttgatttc tccgactggg agcgcttggc ctcgtcgagt tccctgatct
     2761 gcgcgtcgac attctgctcg gagaggcggg ccatctcgtc gaggtgctca aactcggtct
     2821 tgtcgttctc aaactgctgc tggctttttt cgaagctgac gcgcgcggcg gcgccctccc
     2881 ggaagaggag ttgctggcgg aggtacgtct tctcggattt gtcggcgtcg ttccgagcgc
     2941 ggctcgcgtc ggcgcgtgca cgggacgagt caaggcgggc ggcgatgagc cggctctcca
     3001 ggctgctcac ccgcgcctgt gcccgttgga gttccgtgag cgccgcatcc cgctcggatt
     3061 caagcgccgt gttggcgatc tgagcgagca actgtccctc gtacacgctc tggccgacgt
     3121 cggcgaggaa cgactcgacg gccccgtcga ccgggaccgg cacgttgacg attttccgtg
     3181 cctgaatgcg gcctctgagg cttgcttccg tcccggcgaa tgcaggcgga gcggcggcgg
     3241 cagtcttctt cggcggcgcc ggtccgctct cgcgccgaag cagcgacaga gcgcccacgg
     3301 cgacagcaat cagaatcagg gtcgccgaga gaagcagcca tttaccccgc atacccgctc
     3361 atattgtagc cggaagcagg caagcctgag cggcggcgga cggctacaat ggtcgtttca
     3421 tgtccctgcc tcaagcggcg gccgagttca ttgcagcgca agtggagcgg ttgcgacgtt
     3481 ccgtgaaaag gaagcggatc gtcttcccgg agtcctctga tccgcgcatc cttgccgcgg
     3541 cgggccgtct cgcgcaggag ggtctgctcg aaccggtgtt gatcggaccg gcgccgtctg
     3601 gagcgccagc caacgtgacg tttgttgttc cggagtcatc gcctaaactg cgcagctacg
     3661 cggcaatcta cttcgaccgc cgccgcgcca ggggtgtcac cgaagccgag gcaatggaga
     3721 tcgcccggac gccgctgtat ttcgcgaagc tgatggtagc cgcgggcgac gccgacggca
     3781 gcatcggcgg ggcctcgatc tcctcggccg aggcggtgcg ggccgcactc cagtgcatcg
     3841 gcgttcctcc gcacatccga acagtctcga gtttctacat catggccctg cacgaccgcg
     3901 ctttcgggca caaaggcctg ctgttcttcg ccgactgctc gatcgtcatc gacccgagcg
     3961 cggtgcaact ggcggacatt gccatcgcta ccgccggaag cgtccgcaac ctgct
//
LOCUS       DATIFQ010000340        14336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2219134, whole genome shotgun sequence.
ACCESSION   DATIFQ010000340 DATIFQ010000000
VERSION     DATIFQ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14336
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219134"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VLE22_15725"
     CDS             complement(<1..201)
                     /locus_tag="VLE22_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15906.1"
                     /translation="MLNPACRRVAILLLFLLAVAGAWFATSGAQPSVPPVSPIPTEEG
                     QLVVFDKSTWQMRELTKRIPLND"
     gene            complement(263..874)
                     /locus_tag="VLE22_15730"
     CDS             complement(263..874)
                     /locus_tag="VLE22_15730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB15907.1"
                     /translation="MSIHSRFFSTCFAAFFIVVAGVPSPAQQREPGLYAKFDTSMGEI
                     VAKLFEKQAPQTIKSFVDMAEGRKEFLDASGVKVKKPAYDGTVFHRVIPDFMIQGGDP
                     TGTGTGDPGFTIPDEFDDSLKFDIPGRLAMANVGDPNSGTCQFFFTDAPLPYLDGKHT
                     IFGQVITGQDVVRKIARVPRDADDRPKQDVKLNRVVIERVEAK"
     gene            914..1936
                     /locus_tag="VLE22_15735"
     CDS             914..1936
                     /locus_tag="VLE22_15735"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSB15908.1"
                     /translation="MRRRTVQVIGMAAIMVAFSLSVAAQDTRNFPTLGKVVRLDPRID
                     KLIPVDAKIEVLSSGFDWSEGPVWIKNGGFLLFSDIPRNSVMKWKEGEGISVWLKPSG
                     YTGQEPYGREPGSNGLAVDAQGRLISCEHGDRRISRLEPGGGKRTLADNYMGKRLNSP
                     NDLVVKSNGDIYFTDPPYGLPKGWDDPRRELDFCGVYLLSTDGKLTLLTKELVRPNGI
                     ALSPDEKTLYVAQSDGKRPIWMAFPVKPDGTVDAGRVFADASDMIGKLPGGPDGMKVD
                     KAGNLWVTAPGGVHVYSPDGKRLGWIETNERTANCAWGDDGSTLYMTADMYLCRIKTT
                     TKGIGW"
     gene            2049..2459
                     /gene="mscL"
                     /locus_tag="VLE22_15740"
     CDS             2049..2459
                     /gene="mscL"
                     /locus_tag="VLE22_15740"
                     /inference="COORDINATES: protein motif:HMM:NF010557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HSB15909.1"
                     /translation="MWSEFKEFIKRGNVMDLAVAVIIGAAFGKIVGSLTDDLLMPVIS
                     LFTGKIDFANLFFALDGKVYPTLAEAKKAGAATLAYGNFINAVVNFLIVALAVFLLVK
                     QVNRLMPAPPPPPPPGPSPTEQLLGEIRDLLRQR"
     gene            2713..3048
                     /locus_tag="VLE22_15745"
     CDS             2713..3048
                     /locus_tag="VLE22_15745"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB15910.1"
                     /translation="MENVDRRRSARYPIEREIRFVVSTRRKINNDIGTGRTINISSNG
                     ILFSTDQHVIAGSRIKLAVDWPVRLEDNCPLKLVAFGRVVRCDSGTTAVEIEQYEFRT
                     RGNQAFDFP"
     gene            complement(3081..4799)
                     /locus_tag="VLE22_15750"
     CDS             complement(3081..4799)
                     /locus_tag="VLE22_15750"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSB15911.1"
                     /translation="MSTTTPQLAPSLSGASPRFDFELLVTHGDGQSRVVPLISGEVTV
                     GRSPSNTLAFPQDFRLSRSHLSLKKEGDEWVVRDLGSTNGTEVNAQRLAGPHVLRPGD
                     HIVAGDLVLDFQAAERQNLNSTVWFIGGPSPSDSSQVVVDLQSVIGEPEQAASGSAAS
                     ALSGSRVVQALIRAGQELAGHGRLEDLFEVILDLAIQSVDGERGVLMTLDGEQLVVRS
                     SRGEGFQISSAVRDRVIQEKASILVRDAMLDDKLSLRQSIVLQHVRSMIVVPLQTRDR
                     VIGLIYVDKSDIMAPFNDQDLSLLTVMANVAAIRLEQARLAHVEQAQQEMARELAQAG
                     EIQSSLLPKQLPEVEGLDIAGHNVPCRGVGGDYYDCIPCRDGRIVVTVGDVSGKGMPA
                     ALLMTSLQAMISVLVRSVSSPAEMMETLNRNILERCPSNRFITLFLAVIDPFTGGISF
                     CNAGHNRPVIVRRDGRTDLLKDGDLILGISPKAEYSDHRALIRPGDVLVMYSDGVTES
                     TGPGEEAEEFGDERLAQVVAAHRAEDSKAVMEHVLHDLHEYTDGAPFADDVTLVVVKR
                     DANAEA"
     gene            4925..5584
                     /locus_tag="VLE22_15755"
     CDS             4925..5584
                     /locus_tag="VLE22_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15912.1"
                     /translation="MARRRRNLKVVRWIAGTSAEIARETARPALLAIPSAVCARLGGI
                     SVELKGALEVPDASSRWRLSGTGVHIEVATDASSPHDVAIEALLCAGQALWERITDRE
                     LGAWLATLSGEISAAVPGEIDEAPHGLKKALLATAGQPSQRRMLEYARASFAATFAEY
                     VHCLWHDVTVRSGPEHLPARFLRKRLELLARWFPPDPGYHLFPRCPVRRRSARGTLES
                     K"
     gene            5601..7094
                     /locus_tag="VLE22_15760"
     CDS             5601..7094
                     /locus_tag="VLE22_15760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB15913.1"
                     /translation="MTSAYFIVAAWLACLPAQLLPAQTGDAAKLHRGRELMATGKTEE
                     ALAVYEELVRASPDDPSLRTNLAIAQFKAGRFRQVVENCEQVLRAQPDLWTANLFLGA
                     GYVELGEPARAVDPLEKVLAARPEERNALILLAGAQLSLERYEAAMLQYRKSSELSPE
                     SPAVWYGLGQSLEGLARTAAAELAGKYPDSEFHAVVTADVQLKQKLYGGAFRNFQRAL
                     AHQPVLGGLYAGLANLYRETGHEDWAKSAEAKERALPPPDCARSRAACDFAAGRYWEV
                     LDATKAGNSAEALYWTSRASQELAGQAYARLAKLPPSAESHELAARELDRRGRNLEAA
                     KEWRQALSLSPRSRRLKVGLAWSLYMGRDHKAVLELLDGLLREDPDSVELNFLHGASL
                     ASTEQFDKAIPSLEKALSRDPTFLPARAALGQAYLRVGKAEQAIPHLKASLSTDEDGS
                     RHYQLVRAYQALGRLEDAREALAEHQRLLQAIAERTRQEHGSEITAP"
     gene            complement(7106..7639)
                     /locus_tag="VLE22_15765"
     CDS             complement(7106..7639)
                     /locus_tag="VLE22_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15914.1"
                     /translation="MIWLAVLLFQAAAGTPQTEPAQATTFSGPPQAQSGYKLFFESTK
                     GQPCGACHVLAGRGTPAGPDLKAVARLPARAFVMAIKSTRTQYVQKVEPKTGDPFPAM
                     KVKEDDKVVELYDLSKTPPELRKMEKAEIGSMKDNDSWKHPPSSLEYTNEELADIIAF
                     IKWVGYRHSAGVKASEL"
     gene            7798..8703
                     /locus_tag="VLE22_15770"
     CDS             7798..8703
                     /locus_tag="VLE22_15770"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15915.1"
                     /translation="MRNTRILFTATLVAFSIASSARAQLGLFSREQRVMFTPEWKGER
                     FDDGRPKVPDSILQRMGEVTAEEAWGVLRGAKYNLQFEGNWQQIITVPGKRLVGRVVT
                     AVFMPVRPDVNSVIAAKGKEEGRVQTGGQNSWVIDTLKPGDVLVVDMFGKIKDGTFIG
                     DNLGTSIFTKTGTGLVVNGSVRDLSGIQEIKGFTGYVRGFHPSAIADVMLMGINVPIR
                     IGEVTVMPGDVVLGDPEGLTFIPPHLAEKVVDSSEDVRFRDEWGHQMLREGKYTPGQI
                     DSKWTQKMEQEFRQWAEQRRRTPKK"
     gene            8729..12073
                     /locus_tag="VLE22_15775"
     CDS             8729..12073
                     /locus_tag="VLE22_15775"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HSB15916.1"
                     /translation="MCRYRALLSLIVLLIAAPAVFGAAPVRIWEQDIVIPTYPIGPAE
                     PNPMFYFGRVYQGARGAIYPYPLYDKLTDRKEDKTYKAVYLENEYVKICVLPELGGRI
                     FEAVDKTNNYDFFYRQHVIKPSLIGMLGSWISGGVEWNVPHHHRASSFMLVQHKVEEN
                     ADGSKTVWIGELELRHRMRWAIGLTLRPGRSYLEASFRLLNRTPLVQSLLCFANVAVH
                     TNENYQIIFPPSTQFITQHAKREFSRWPVGDSSYAGIDYRGVDETWWKNHPNPVSMFA
                     WNYEDDFVAGYDHGKQAGTMSVADHNVVPGKKFFAWGNGPAGEMWDKILSDEDGPYLE
                     LMVGAYSDNQPDYSWIQPYEAKSFKQYWYPFRNIGGVKNANLDASVNLEVKGTSADLG
                     FYTTAAFPDATVLLKAGEKVVLEEKISIAPDKPYTKKVSLPAGVEPHTLRASLSAGGR
                     ELIGYTPVRVTKEPMPAPVEPPPPPKEVKTTEELYLAGLRLEQFHSPALDPDPYYEEA
                     LNRDPGDSRANVALGTLYLKRGRFADAEKLFRSAIARLSKNYTSPKDGEAYYYLGVAL
                     KMQGKLDEAYTAFYKGIWSAAWQPAGWYSLAEIAAVRGDMPLALDFADRAIASNAMNT
                     RAINLKAAILRKMGRSAEAVQLAKAVTDTDPLDMRAVAERWLADKKSGVARELRTLLA
                     EHPNTGLETAVELGNAGMYDDAIDVLREMASLSPDRKSVSPMVFYYLGYYADKVKRPM
                     DCRKLAATQPAEYVFPFQLEAIAALRHAMEADPKDARAPYYLGNLLFDLQPETAVELW
                     EKAQQLDPSFPVVHRNLALAYARQEGGLDKAIASLEKAISLNGDDPVFLFELDQMYET
                     AGASPEKRLAMLEKRRETVLKRDDAISRYIELLVLAGRYDDAIGMLQGRTFHIWEGGA
                     RFGVHGSWVDAHVLRGQQRLATGKHQQALEDFQAALDYPQNLQTARPKRGGRFPEIYY
                     HIGMAQEAMGNAQKAREAWQASVAQTVGRDEDLDVLANTATAMRYYQARSLEKLGEKQ
                     KATRIYQALLKSGNEGMKETSTVDYFAKFGERQSRRAKLANAHFTLGLGYLGKNDRAK
                     AKQEFAQALEQSPDHLAAKTNLSALD"
     gene            12110..13669
                     /locus_tag="VLE22_15780"
     CDS             12110..13669
                     /locus_tag="VLE22_15780"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HSB15917.1"
                     /translation="MITRIAGALVVCLGLLSAAQPPILTPKPSPKPRINGARIFGVRP
                     GRPFLHTIAATGQRPMTFSAKGLPAGLTLDQKSGRISGFLSKPGEHVVTLRAANSLGA
                     AERRFRIVVGDKIALTPPMGWNSWYAWSESVSDEKIRSVARGFVAKGLVNHGWTYVNI
                     DDCWQGLRGGKYNAIQGNERFRDIKGMCDYVHGLGLKIGIYSTPWMATYGGFIGGSAP
                     TAKGDYSGMWLPPEKRLQPYQVFGRWPSSQRLGAQKLGAYWFSDADAKQWAEWGIDYV
                     KHDWKPNDPPTTERILKGLLACGRDIVLSLSNEAPFEHAAEWARLSNLWRTTGDIKAT
                     WEAVSRIGFTQDRWAPYAGPGHWNDPDMLQIGMIGVPNTYVRTLKPTVLTPGEQYTQV
                     SLWSLLAAPLLLSCDIETMDDFTLSLVTNDEVIDVNQDPLGKSGVPVSRAGGLEVWAK
                     QMEDGSQAVGLFNRSDQEATVTARWSDLGVRGKQEVRDLWRQKDLGVFEGQFQTKVAG
                     HGVVLVRVSAR"
     gene            complement(13681..14061)
                     /locus_tag="VLE22_15785"
     CDS             complement(13681..14061)
                     /locus_tag="VLE22_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSB15918.1"
                     /translation="MTSSLSGKPIRRRLVRAALGQEFILAAGVVLVFCANSTHNAQRY
                     GERGRRLYAVQDATVACTYAMLAAAALELGTVWVGAFNDDAVRQVIDAPENVTPVAIL
                     PIGYPAETPDRTPRRPLGKLVHEL"
     gene            complement(14015..14194)
                     /locus_tag="VLE22_15790"
     CDS             complement(14015..14194)
                     /locus_tag="VLE22_15790"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSB15919.1"
                     /translation="MDYFEAVRKRQSIRAYQPKPVEEEKLLRILETANAAPSAGNLQA
                     YDIFVVRKADQKKAG"
BASE COUNT         2820 a         4301 c         4529 g         2686 t
ORIGIN      
        1 atcgttgaga ggaatgcgct tggtcaactc gcgcatttgc caggtgctct tatcgaacac
       61 gacgagttgc ccttcttcgg tcggaattgg gctaacggga ggcacgctgg gttgagcgcc
      121 ggaggtcgca aaccaagcgc ccgcgacggc gagcagaaat aacagcagga ttgccacacg
      181 cctgcaggca ggatttagca cggtgattct tctcctccgc gccagtgtcc ggcagcggcg
      241 gaggctgtgt caagccgtgc ggttacttcg cctcgacgcg ctcaatgaca acgcggttga
      301 gtttgacgtc ctgtttcggc cggtcgtcgg cgtcgcgcgg gacgcgggcg atcttgcgca
      361 ccacgtcctg ccctgttatc acctggccga aaatagtgtg cttcccgtcg agatagggaa
      421 ggggagcgtc ggtgaaaaag aactggcagg taccgctgtt cggatcgccg acgttggcca
      481 tcgccagacg acctgggatg tcaaacttga gggaatcgtc gaactcgtcg ggaatcgtga
      541 aaccgggatc acccgtgccg gtgcccgtcg ggtcgccacc ctgaatcata aagtccggga
      601 tgacgcgatg gaaaaccgtt ccgtcgtagg ccggcttctt cactttgacg cccgaggcgt
      661 cgagaaactc cttgcggcct tcggccatat ccacgaagct cttgatcgtc tgcggtgcct
      721 gcttctcgaa gagtttggcg acgatctctc ccatgctggt gtcgaatttc gcgtagagtc
      781 cgggctcacg ctgctgggct ggcgagggta caccggccac gacgatgaag aatgcggcga
      841 aacacgtcga aaaaaagcgc gaatggatgc tcatctatcg gtgattatac tcctggggta
      901 atatggggag gccatgagga gacgcacggt gcaagttatc ggaatggccg cgattatggt
      961 ggcattctcg ctatcggtcg ctgcccagga cacgcggaac tttcccactc tcggcaaggt
     1021 cgttcggctg gatccgcgga tcgacaagct gatcccggtc gatgcgaaga tcgaggtcct
     1081 gtcttcgggc ttcgactggt cggaggggcc ggtctggatc aagaatggtg gcttcctcct
     1141 gttctcggac atcccgagga actcggtcat gaagtggaaa gaaggcgagg gtatcagcgt
     1201 ctggttgaag ccgtccggct acaccggaca agagccttac gggcgcgaac cgggctcgaa
     1261 cggactcgcg gtggacgcgc agggacggtt gatctcctgc gaacatggcg accgccgcat
     1321 ctcgcggctg gagccaggcg ggggaaaacg gacgctcgcg gacaactata tgggcaagcg
     1381 cctcaatagt ccgaacgacc tcgtcgtgaa gtcgaacggc gatatctact ttaccgaccc
     1441 gccgtacggc cttccgaaag gctgggatga cccgcggcgc gaactcgact tttgtggtgt
     1501 atacctgctc tctacggacg gcaaactcac gcttttgacg aaagaactcg tgaggccgaa
     1561 cggcatcgcc ctctcacccg acgagaagac gctctacgtg gcgcaatcgg acggcaagcg
     1621 gcccatctgg atggcgtttc cggtgaagcc ggacggcaca gtggacgcag gacgggtttt
     1681 cgcagatgca agcgacatga tcggcaaact accgggtggc cccgacggca tgaaagtgga
     1741 caaggcgggc aacctttggg tgacggcgcc tgggggcgtg cacgtgtatt cgccggatgg
     1801 caagcgactc ggctggattg aaactaacga gcgcaccgcc aactgcgctt ggggtgacga
     1861 cggttcaact ctctacatga ccgccgacat gtacctctgc cggatcaaaa cgaccaccaa
     1921 ggggataggt tggtaggtct agctccgggc ctgttacatt ttgtactacc cttgatgccg
     1981 cttttgaggt agacttcgtc gatcgggaat tcacgttgga ctgacgcaag cacattaagg
     2041 agaccgatat gtggagtgag ttcaaggagt tcatcaagcg tggtaacgtc atggaccttg
     2101 cggttgccgt aatcatcggc gccgcgttcg gaaaaatcgt cggttcgctc acggacgacc
     2161 ttctgatgcc cgtgatcagc ctgttcacgg gcaagatcga tttcgctaac ctcttctttg
     2221 ccctggacgg aaaggtctac ccaaccctgg cagaagccaa gaaggccgga gcggctacgc
     2281 tggcgtacgg caatttcatc aacgccgtcg tcaacttcct gatcgtcgcg ttggccgtgt
     2341 tcttgttggt gaagcaggtc aaccggttga tgccggcgcc tccgccgccc ccgcctccgg
     2401 gtccttcgcc aactgagcag ttgttgggtg agattcgcga cctcctcaga cagaggtagt
     2461 gtcttcgccg gggcgaggct tacctcgccc ctccccttcc cggctctgga atgttgctat
     2521 caccctgacg taaaccgatc cccaaagtcg ggatgttcac gaccgtcgcc cccggtacta
     2581 gtactcacca aaccatttgt actgtaggtc gcaccatttc gtactattcc ctaactgtac
     2641 aacggtccgt catgctgtta gaggagatgt cctgactgaa taagtgagct gctgaagcgg
     2701 ggagccacag agttggaaaa tgttgaccgg cgccgttcgg cgcggtatcc aatcgaacga
     2761 gagatccgct ttgtcgtttc cactcggagg aagatcaaca acgacatcgg taccggaaga
     2821 actatcaata tcagcagcaa cggtattttg ttctcaaccg accagcacgt aatcgccggg
     2881 tcgaggatca aattggcggt cgactggccg gttcggctgg aggacaactg tccattgaag
     2941 ttggtggcct tcggccgggt cgtgcgctgc gacagcggta ccaccgcagt tgagatcgag
     3001 cagtacgaat tccgcacacg tggaaaccag gcgtttgatt ttccctgatt cgcaccgctt
     3061 gacggagcct ggacggcacg ctacgcctca gcgttcgcgt cgcgcttcac gacgaccaaa
     3121 gtcacatcgt cggcgaaggg cgctccatcg gtgtattcat ggaggtcatg gaggacatgc
     3181 tccataaccg ccttggaatc ctcggcgcgg tgcgctgcga ccacctgcgc gagccgttcg
     3241 tccccaaatt cttccgcctc ctcccctggt ccggtcgatt cggtgacacc gtcgctatac
     3301 atgacgagaa catcgccggg ccgaatcagg gcgcggtggt cggaatactc cgctttcggg
     3361 ctgatgccca ggatcaagtc gccatccttc aggagatcgg tccggccgtc gcggcggacg
     3421 atgaccggcc ggttgtgtcc ggcgttgcag aaggagatgc cgccggtaaa cgggtcgatc
     3481 acggccagga agagcgtgat gaagcgattg ctcgggcagc gctcgaggat gttgcggttc
     3541 agcgtctcca tcatttcggc cggtgagctg acggaccgca ccagaaccga gatcatcgcc
     3601 tggaggcttg tcattagcaa cgccgccggc attccctttc cggaaacatc gccgacggtc
     3661 accacgatgc gcccgtcgcg gcacggaatg caatcgtagt agtcgccgcc gacgccccgg
     3721 caaggaacgt tgtgacccgc gatgtcgagg ccttccacct cggggagttg cttcggtaga
     3781 agactgctct ggatttcgcc ggcctgcgcg agttcccgtg ccatctcctg ttgcgcctgt
     3841 tcgacatggg cgagccgcgc ctgctctagg cggatggccg caacattcgc catgacggtc
     3901 agcaggctga ggtcctgatc gttgaacggc gccatgatgt cggacttgtc gacatagatc
     3961 aggccgatca cgcggtccct cgtctggagg ggcaccacta tcatgctgcg gacgtgctga
     4021 aggacgatac tttggcggag gctgagcttg tcgtcgagca tcgcgtcacg gacgaggata
     4081 gacgcctttt cctggatcac acggtcgcga actgcgctgc tgatctggaa cccctcgccg
     4141 cggctggagc ggacgacgag ttgttcgccg tcgagcgtca tcaagacccc tcgctcgccg
     4201 tcgacgcttt gaatcgcgag atccagaatg acctcgaaaa ggtcttctag ccggccgtga
     4261 cctgcaagct cctgtccggc gcggatgagc gcctgcacca cacgggagcc cgaaagtgca
     4321 ctggcggccg aacctgacgc cgcctgctcc ggttccccga tcacgctttg caagtccacc
     4381 actacctggg acgaatccga aggagacggt ccgccgatga accataccgt gctgttgagg
     4441 ttctgccttt cggctgcctg aaaatcaagt acaagatcgc cggccacaat atggtcgccc
     4501 ggccgcagaa cgtggggacc tgccagccgt tgcgcgttca cttcagtgcc gttggtgctg
     4561 ccgagatccc gcacgaccca ctcgtcgcct tctttcttca gcgaaaggtg gcttcgcgac
     4621 aagcggaagt cctggggaaa cgccagggtg ttggaaggcg atcgtccgac ggtcacctca
     4681 ccggagatca gggggacgac gcggctctgc ccgtcaccat gggtgacaag aagctcgaaa
     4741 tcaaacctgg gcgaggcgcc tgacaaggac ggagcgagtt gcggcgttgt cgtggacata
     4801 acgtgcgccc aggagcgatc gcgtctgttt gaagcttgtc acgggcaccg agcagaagtc
     4861 aaggcggaca gatgcaaacg gacaaagaac ggcgctcatc agacacaata tggttactgg
     4921 actcatggcc cgccggcgca gaaacctgaa ggtagtgaga tggatcgccg gaacgtccgc
     4981 ggagatcgcc cgcgagacgg cgcggccagc gctgctcgcg attcccagcg cggtctgcgc
     5041 ccgactggga ggcatttccg tcgaacttaa aggtgcgctg gaagtgcccg atgcttcctc
     5101 ccgatggcgc ctgtccggga ccggggtcca catcgaagtc gccaccgacg cgagtagtcc
     5161 gcacgacgtt gccatcgaag cgctgctctg cgctggccag gcgctgtggg agcggatcac
     5221 cgatcgcgag ctcggggcct ggctggcgac tctcagcggc gaaatctcgg cggctgtccc
     5281 gggcgagatc gacgaggctc cgcatggact caagaaagcc ttactcgcaa cggccgggca
     5341 accttcccag cggcgcatgc tcgaatacgc ccgcgcatcg tttgcggcga cgtttgccga
     5401 atacgtacac tgcctgtggc acgacgtcac cgtgcgctcc ggtcccgagc atctccccgc
     5461 gcgtttcctc cgaaaaaggc tggaactgct ggcgcgatgg ttcccgccgg acccgggata
     5521 tcacctgttt ccgcggtgtc cggtccgccg gcgttccgcc cgcggaacgc tagaatcaaa
     5581 gtgagagcat cgtcttcgcg atgacgtccg cgtacttcat cgttgccgcg tggctggcct
     5641 gtttgccggc ccagcttctg cccgcccaaa caggcgatgc ggcgaagctc caccgcggac
     5701 gggagctgat ggcgacgggc aagaccgaag aagctcttgc ggtttacgag gaactggtgc
     5761 gcgcctcgcc ggacgaccca tcgctgcgga ccaacctggc catcgcgcaa ttcaaggcgg
     5821 gacggttccg tcaggtcgtc gaaaactgcg aacaggtgct aagggcacag cccgatctgt
     5881 ggactgcgaa tctatttctc ggagcaggct atgtcgaact gggagagccg gcgagagccg
     5941 ttgatcctct cgagaaagtt ctcgcagcca ggcccgaaga acgaaacgcc ctcattttgc
     6001 tggcaggagc gcagttgtcg ctcgaacggt acgaggcagc gatgcttcag taccggaagt
     6061 cttcggagct gtcgccggaa agcccggcgg tatggtacgg gcttggacag agcctcgagg
     6121 gattggcgcg cacggcggcg gcggaactgg cagggaagta cccggattcc gagtttcacg
     6181 ccgtggtgac ggctgacgtg caactgaaac agaagctgta cggtggcgca ttccggaact
     6241 ttcagcgggc gctggcgcat caaccggtgt tgggcggctt gtacgcgggc ctggcaaatc
     6301 tttaccgaga gaccggccat gaggactggg cgaagtcggc cgaggcgaaa gagcgcgccc
     6361 tcccgccgcc cgactgtgcc agatcgcggg cggcttgcga cttcgccgct ggccgctact
     6421 gggaggtgct ggacgcgacg aaagcgggga actccgccga ggcgctctat tggacgtcgc
     6481 gcgcctctca ggaactggct ggccaggcgt acgctcgact cgccaagttg ccgccatccg
     6541 cagagagtca cgaacttgcg gcccgcgaac ttgaccggcg cggccggaat ctggaggcgg
     6601 ccaaggagtg gcggcaggcg ctatcgcttt ctccgcggag ccgccgtctc aaagtcgggc
     6661 ttgcgtggtc cctctatatg gggcgggacc acaaggccgt gctggaattg ctggacgggc
     6721 tcttgcgcga ggatccggat tcggtagaac tcaacttcct tcatggcgca agtctggcca
     6781 gcacggagca gttcgacaag gccattcctt ccctcgaaaa agcgctttcg cgggacccca
     6841 cgttccttcc ggcgcgagcc gctctcggac aggcttacct ccgcgtcgga aaggcggagc
     6901 aggcgatccc gcacctgaag gcgtcgttat cgacggacga ggacggcagc cggcactatc
     6961 agcttgtccg cgcatatcag gcgctcggcc ggctggaaga tgcccgcgag gcccttgcgg
     7021 agcatcagag actccttcaa gccatagcgg aacgaacgcg tcaggagcac gggagcgaga
     7081 tcaccgcccc gtagaccagc gccgcttaca actcggatgc tttcacgccc gccgagtgac
     7141 ggtagccgac ccacttaatg aaggcgatga tgtccgccag ttcttcgttg gtgtactcga
     7201 gcgagcttgg cgggtgcttc caggaatcgt tgtccttcat ggagccgatt tccgccttct
     7261 ccatctttcg gagttcgggc ggcgtcttgc tgaggtcgta caattcgacg accttgtcgt
     7321 cttccttgac tttcatcgcc gggaatggat ctccggtttt gggttcgacc ttctgcacgt
     7381 actgcgtccg ggtcgatttg attgccatga cgaacgcccg agcagggaga cgggcgacgg
     7441 ccttgagatc gggtcccgca ggtgtaccgc gcccggccaa gacgtggcac gccccgcaag
     7501 gctggccttt cgtgctctca aagaagagtt tgtagccgct ctgggcctgt ggcggtcccg
     7561 agaacgtcgt tgcctgcgcg ggttccgtct gaggcgtacc ggcggcagcc tgaaagagaa
     7621 ggacagcgag ccatatcatg agttccctcc acggtggagc gatgtcgcgc tccggcatca
     7681 ttatgccacc aagagggccg cgcgcctagg gtcggctgct gcgttagagg cagtacaggg
     7741 cgccgcttct tgacatccct cgatcgcttt catagcgtag agtcttcaag gaatactatg
     7801 cggaataccc ggatcctatt cactgccacc ctggtggcgt tttcgattgc gtcctcggcc
     7861 cgggcgcagc tcggtctgtt ctcgcgcgag caacgcgtca tgttcacgcc cgagtggaaa
     7921 ggcgagcgat tcgacgatgg acgcccgaag gtgccggact cgatccttca acgcatgggc
     7981 gaagtcaccg ccgaggaggc ctggggtgtc ctgcgcgggg ccaagtacaa cctccagttc
     8041 gaagggaact ggcagcagat catcaccgtt cccggcaaaa gactggtcgg ccgcgttgtg
     8101 actgcagtgt tcatgcctgt ccggcccgac gtgaactcgg tgattgccgc caagggcaaa
     8161 gaggaagggc gcgtgcagac tggcggtcag aattcttggg tcatcgacac gctgaagccg
     8221 ggcgacgtcc tggtagtgga catgttcggc aagattaaag acggaacgtt catcggcgac
     8281 aacctcggta cttcgatttt cacgaagacg ggaaccggac tcgtggtaaa cggcagcgtc
     8341 cgggacctgt cggggattca ggagatcaaa ggcttcaccg gctatgtacg cggctttcac
     8401 ccctcggcca tcgccgacgt catgctgatg gggatcaacg tgccgattcg tatcggtgaa
     8461 gtcacggtca tgccgggcga cgtcgtcctt ggcgatccgg aaggtctcac cttcattccg
     8521 ccgcacctcg ccgagaaggt ggtcgacagc tcggaggacg tgcgcttccg cgacgagtgg
     8581 ggccaccaga tgctgcgcga ggggaagtac accccaggac agatagacag caagtggact
     8641 cagaagatgg agcaggagtt ccgccagtgg gctgagcagc ggagacggac gccgaaaaaa
     8701 tagctaccct acgtaacagg aggtctttat gtgtcgttat cgggccttat tgtcccttat
     8761 tgttttgctg atcgccgctc cggcggtctt cggcgcggcg ccggtgagga tctgggagca
     8821 ggacatcgtt attccgacgt atccgatcgg tccggcagag ccgaacccga tgttctactt
     8881 cgggcgtgtc taccaaggcg cgcggggcgc catctatcca tatccgctct acgacaagct
     8941 gacggacagg aaagaagaca agacgtacaa ggccgtctat ctggagaacg aatacgtcaa
     9001 gatctgcgtg ttgccggaac tcggcggccg gatttttgaa gcggtcgaca agacgaacaa
     9061 ttacgatttc ttctaccgcc agcacgtgat caagccgtca ctcatcggca tgttgggctc
     9121 gtggatctcg ggaggggtgg agtggaacgt accgcatcac caccgcgcgt ccagtttcat
     9181 gctcgtgcag cacaaggttg aggaaaacgc cgacgggagc aagacggttt ggatcggaga
     9241 actggagttg cggcaccgga tgaggtgggc gatcggcctg acgctgcggc ccgggcgctc
     9301 ctacctcgag gcctccttcc ggctgctaaa ccgcactcca ctggtacagt cgctcctgtg
     9361 cttcgcgaac gtggcggtcc acaccaacga gaactatcag atcattttcc cgccgagtac
     9421 gcagttcatc acccagcacg ccaagcgtga gttttcacgc tggccggtag gggactcgtc
     9481 ctatgctggc atcgattacc ggggcgtgga cgaaacgtgg tggaagaatc atcctaatcc
     9541 ggtttcgatg ttcgcctgga actacgaaga cgacttcgtc gcgggttacg accacggcaa
     9601 gcaggcgggc acaatgagcg tggcggatca caacgtcgtg cctggaaaga agttcttcgc
     9661 ctggggtaac gggccggccg gagagatgtg ggacaagatt ctttcggacg aggacggacc
     9721 gtacctggag ttgatggtcg gcgcgtactc tgacaaccag ccggactata gctggattca
     9781 accgtacgag gcgaagtcct ttaagcagta ctggtatccg tttcgaaaca tcggtggcgt
     9841 gaagaacgcc aacctggacg catcggtcaa cctggaggta aagggaacct ccgccgacct
     9901 cgggttctac accacggcgg cgtttccgga tgcaaccgtg ctgctgaaag cgggcgagaa
     9961 ggtcgtgctc gaggagaaga tctcgatcgc tccggataag ccctatacga agaaagtttc
    10021 cctccctgcg ggggtcgagc ctcatacgtt gcgggcctcg ctctcggccg gtggccgcga
    10081 gctgatcggg tacacgccag tccgagtcac aaaggagccg atgccggcgc ccgtcgagcc
    10141 gcctcctccc ccgaaggaag tgaagacgac tgaggaactg tatctcgctg gcctgcggct
    10201 ggagcaattt cacagccccg cgctcgatcc cgatccctat tacgaagaag ccctgaatcg
    10261 cgatcctggc gattcccgcg ccaacgtcgc cctcggcacg ctgtatctca agcgcggacg
    10321 gtttgcagat gccgagaagc ttttccgctc ggcgatcgcc cggctctcga agaactatac
    10381 gtctccgaag gatggcgagg cgtactacta cctgggcgta gcgctgaaga tgcaggggaa
    10441 gctcgacgag gcctacacgg cgttctacaa agggatctgg agcgccgcct ggcagcccgc
    10501 cggctggtac tcgctcgccg aaatcgccgc cgtgcgtggc gatatgccct tggcccttga
    10561 cttcgccgac cgggcgatcg cgtcgaacgc gatgaacacc cgcgcgatca acttgaaggc
    10621 agccattctg cgcaagatgg gccgtagcgc cgaggcggtg caactggcga aagcggtgac
    10681 agacaccgat ccgctcgaca tgcgggcggt cgccgagcgt tggttggccg acaagaagag
    10741 cggcgtcgca cgggagttac ggaccctgct ggccgagcat cccaacacgg ggctcgaaac
    10801 cgccgtcgag ctcggcaacg cggggatgta tgacgacgcg atcgacgtgt tgcgggagat
    10861 ggcctcgctt tcgcctgacc gcaagagcgt ttcgccgatg gtgttctact acctcggcta
    10921 ttacgcggac aaggtaaaac ggccgatgga ttgccgcaag ctggctgcca cgcagccggc
    10981 ggagtacgtg ttcccattcc agcttgaggc gatcgctgca ctgcgccacg ccatggaggc
    11041 cgatcctaaa gacgcgcgcg ccccgtacta cctcggcaac ttgctgttcg atttgcagcc
    11101 cgagacggcg gtcgaactgt gggagaaggc tcagcagctt gacccatcct tccccgtcgt
    11161 ccaccgcaac ctcgccttag cctacgcgcg gcaggagggc gggctcgaca aggccatcgc
    11221 gagcctagag aaagcgatct cgctgaacgg cgacgatccg gtcttccttt tcgaactcga
    11281 tcagatgtac gaaacggcgg gcgcgagtcc tgaaaagcgc ctggcaatgc tcgaaaagcg
    11341 tcgcgaaaca gtcctgaaac gtgacgatgc gatttcccgg tatatcgaat tgcttgtgct
    11401 ggcgggccgc tacgacgacg cgatcggcat gctgcagggc aggactttcc acatctggga
    11461 gggcggcgcg aggttcgggg tccacgggtc gtgggtggac gcacacgtgc ttcgcggaca
    11521 gcagcggtta gcgacgggca agcatcagca ggcgctcgag gacttccagg ccgcgctcga
    11581 ctatccgcag aacctccaga cggcccgccc caagcggggc ggacgtttcc cggagatcta
    11641 ctaccacatc ggaatggccc aggaagcaat gggcaatgct cagaaggccc gggaggcgtg
    11701 gcaggcgtcg gtggctcaaa cggtgggaag ggatgaagat ctcgacgtgc tggccaatac
    11761 cgcgacggcc atgcgttact atcaagcgcg ctcgctcgaa aagctgggtg agaaacagaa
    11821 ggcaacccgg atctatcagg cgctgctcaa gtcaggcaac gaagggatga aagagacatc
    11881 gacggtggac tatttcgcga agttcggcga gcggcagtcg cgccgcgcaa agctcgcgaa
    11941 cgctcacttt acactcgggc ttggctacct cggcaagaac gaccgagcga aggcgaagca
    12001 ggagttcgca caagcccttg agcagagtcc cgaccacctg gcggcaaaga cgaacttgtc
    12061 ggcactggac tgagacgcat cgaccccaac aggcaaagga cacaatggca tgattacccg
    12121 aatcgcggga gcgctcgtag tttgtctggg cctgctttcc gcggcccaac cgccgatcct
    12181 gacccccaaa ccgtcgccga agccgcgcat caacggcgca cgcatctttg gcgtgcgtcc
    12241 cgggcggcct tttctccata ccattgctgc gaccggccag cgtccgatga ccttctcggc
    12301 gaaaggtctg cccgcgggcc tcaccctcga ccagaagagc ggccgcatca gcggctttct
    12361 ttcgaagccc ggcgagcatg tcgtcaccct ccgcgccgcg aactccctcg gcgcggccga
    12421 acgcaggttt cggatcgttg taggcgacaa gattgcgctc acgccgccga tgggatggaa
    12481 cagttggtac gcatggtccg aatctgtgag cgacgagaag attcgctcgg ttgcgcgcgg
    12541 gttcgtcgcc aagggtctgg tcaatcacgg atggacgtac gtgaacatcg acgactgctg
    12601 gcagggtctt cgcgggggca agtacaacgc gatccagggc aatgagcgat tccgcgatat
    12661 caaggggatg tgcgattatg tccacggtct cggcctgaag atcggaatct actcgacgcc
    12721 gtggatggcc acgtacggag gcttcatcgg cggcagcgcc ccgacggcaa aaggcgatta
    12781 ctccggcatg tggctaccgc cggaaaagcg cctccagcct taccaggtat tcggacgctg
    12841 gccaagttcc cagcgtcttg gcgcgcagaa attgggagca tactggttct ccgacgccga
    12901 cgcgaagcag tgggccgagt ggggcatcga ctatgtgaaa cacgactgga agccgaacga
    12961 tccccctacc acggagcgca tcctcaaggg actgctggcg tgcggccggg acattgtcct
    13021 cagcctctcg aacgaagcgc cgttcgaaca tgcggccgag tgggcgcggc tttccaatct
    13081 ctggcgaacc accggcgaca tcaaggccac atgggaggcc gtgagccgca tcggcttcac
    13141 gcaggaccgc tgggctccct atgcgggtcc cggccactgg aatgatcccg acatgcttca
    13201 aatcggcatg attggcgtgc cgaacaccta cgttcgcacg ctgaaaccga ccgtgctgac
    13261 cccgggcgag cagtataccc aggtaagcct ctggagcctg ctggcggcgc cgctgttgct
    13321 gagctgcgac atcgagacca tggacgactt cacgctgagc ctggttacca acgacgaggt
    13381 gatcgacgta aaccaagacc cgctcggcaa gtcgggggtg ccggtctctc gcgccggcgg
    13441 cctcgaggtt tgggcgaagc agatggagga cggcagccag gccgtcggtc tgttcaatcg
    13501 ctcggaccag gaagccacgg tgacggcgcg ttggagtgac cttggcgttc gcggcaagca
    13561 agaggtacgt gacctgtggc ggcagaaaga cctcggcgta ttcgaagggc agttccagac
    13621 gaaggtggct ggccacgggg tcgtgctagt cagagtttcg gcgcgctgac gcgtcgccgc
    13681 tcagagttcg tgcacaagtt tgccgagcgg gcggcggggg gtgcggtcgg gcgtctccgc
    13741 cgggtagccg atcggcagga tcgccaccgg cgtgacattc tccggcgcgt cgatcacctg
    13801 ccgcacagcg tcgtcgttga aagcgccgac ccagaccgtc cctagctcca gggccgccgc
    13861 ggccagcatt gcgtaggtgc aggcgacggt cgcgtcctgt acggcataca gacggcggcc
    13921 acgctcgccg tacctctggg cattgtgcgt cgaattcgcg caaaatacca gcaccacccc
    13981 ggcggccagg atgaactcct gccccagcgc cgccctaacc agccttcttc tgatcggctt
    14041 tccggacaac gaagatgtca tacgcctgaa ggttgcccgc cgacggcgcg gcattcgccg
    14101 tttccagaat tctcagcaac ttctcttctt ccacgggctt cggctgatac gcccgaatcg
    14161 actgccgctt tctgaccgct tcgaagtaat ccatggcgca ccttggctga cgcggagttc
    14221 ggactcctat atactcctac tcgggagtct taagctcaat atgatccgta ttctctcagc
    14281 acttctcttt ctcctgccgt gtctgcccgc ggccgcgcag accagacgca tcgtat
//
LOCUS       DATIFQ010000341         3574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1645047, whole genome shotgun sequence.
ACCESSION   DATIFQ010000341 DATIFQ010000000
VERSION     DATIFQ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645047"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VLE22_15795"
     CDS             complement(<1..811)
                     /locus_tag="VLE22_15795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB15920.1"
                     /translation="MTPSRRWTIIGLLCLGMVIAYFDRVNLSIALASQDFKNFFDLTD
                     QDRGLLNSAFFWSYAFLQIPAGWVADRFGVKFPYAAGFLLWSVMSALAALAGSLWQLF
                     ALRMLLGVFESIVTPAGMRWIRFHCAENQRGLAVGLYMAAAKAGPALGAPLATWLLVA
                     YGWRPMFVILGLGCLVWLLPWMLIVEDDDRKIERAAVQKAAAAVPFLRVLASPVIWGT
                     IIGTFCYQYFVYFCMTWMPAYFVERRNLSLESMGWYTMFSFMGMAVVATAAG"
     gene            972..2225
                     /gene="glgC"
                     /locus_tag="VLE22_15800"
     CDS             972..2225
                     /gene="glgC"
                     /locus_tag="VLE22_15800"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HSB15921.1"
                     /translation="MELRVLAFVLAGGKGTRLYPLTKERAKPAVPFGGKYRIVDFVLS
                     NLINSGIYSIYVLIQFKSQSLLQHLRDGWQFSGLLNDYFIIPVPAQMRITGETWYRGT
                     ADAMYHNLNLIEQSHPDLVVVFGADHIYRMNIRAMVEYHREKKAEVTIAAIPVEKRWA
                     SEFGVIETTADGRIVGFHEKNADAPTIPGDSERVYASMGNYIFSTGRLLHELKEDAEN
                     ESSSHDFGRDILPSLVGQCEMFAYDFDRNPIPGDPPEVRPYWRDVGTIDAYFEAAMDL
                     RSVVPALNLYNKLWPVRTAGYPDPPAKFTFDEDGRRGEAIDSIVSGGSILSGGLVRRS
                     VLHRGVRVHTGAVVEDSIIFDNCDIGRHCKIRRAIIDKNVALPEDSVIGYDLESDERN
                     YHVSEGGIVVVEGGRTPVDIARVRL"
     gene            complement(2351..2599)
                     /locus_tag="VLE22_15805"
     CDS             complement(2351..2599)
                     /locus_tag="VLE22_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15922.1"
                     /translation="MWAKRYYPGMSGTAEIPLYSSDDPAVYVFMHSRDNQVRGILIVQ
                     REFNGEMTAGRFLDRPDSIRYYLDRIRQTQPIEVDWGT"
BASE COUNT          757 a         1094 c         1068 g          655 t
ORIGIN      
        1 agccggcggc ggtcgccacc accgccatgc ccatgaagct gaacatcgtg taccagccca
       61 tcgactcgag cgagaggttg cggcgctcga cgaagtacgc gggcatccag gtcatgcaga
      121 agtagacgaa gtattgataa cagaacgtcc caatgatggt tccccagatg accggactcg
      181 ccagaacccg gaggaagggc accgccgcgg cagccttctg caccgcggcg cgctcgatct
      241 tccggtcgtc gtcctcgacg atcagcatcc agggcagaag ccagacaaga cagccgagtc
      301 cgaggatcac gaacatcggg cgccagccgt aggcgaccag taaccaggtg gcaagcggcg
      361 ctccgagtgc cgggccggcc ttggccgccg ccatgtacaa cccgaccgcc agaccacgct
      421 ggttttcggc gcagtggaag cggatccatc tcatgccagc aggagtgacg atcgattcga
      481 agacgcccag gagcatccgc agcgcgaaga gctgccagag ggagcctgcc agcgctgcca
      541 gcgccgacat gacgctccac agaagaaacc cggccgcgta gggaaacttg actccgaacc
      601 ggtccgccac ccatccggcg ggtatctgga ggaaggcgta agaccaaaag aacgcggaat
      661 tgagcaggcc tcgatcctgg tcggtgagat cgaagaaatt cttgaaatcc tgagacgcaa
      721 gcgcaattga gaggttgacg cggtcgaaat aggcgatgac catcccgagg caaagcagcc
      781 cgatgattgt ccatcgccgc gaaggagtca tagtcgcttc aagagagtgc atcagcagtc
      841 gtcccggtgg acacgaagct cctcaacata tcacacgagg tggatcgcag gatcgatggc
      901 ctggcgcgcg ttcgtgtgac acaatgttgg cttgatgtgc gaagccgtca ccgatccagg
      961 gggtcttccg gatggaactc cgagtgctcg cattcgttct tgcaggcggc aagggaaccc
     1021 gcctctaccc tcttacgaag gagcgcgcca agcccgccgt accctttggc gggaagtacc
     1081 ggatcgttga tttcgtcctc agcaacctca tcaattccgg gatctactcg atctacgttc
     1141 tcatccagtt caagagccag tcgctgctcc aacacctgcg cgacggctgg cagttcagcg
     1201 ggttgctgaa cgactacttc atcatcccgg tgcccgccca gatgcggatc acgggcgaaa
     1261 cgtggtaccg cggaaccgcc gacgccatgt accacaacct gaacctgatc gagcagtccc
     1321 atcccgacct ggtggtcgtg ttcggcgctg accacattta cagaatgaac atacgggcca
     1381 tggtggaata tcaccgggag aagaaggcgg aggtgacgat cgccgccata cccgtcgaga
     1441 agcgctgggc ctccgagttc ggggtgatcg aaacgaccgc tgacgggcgg attgtcggct
     1501 ttcacgaaaa gaacgcggac gccccgacca ttccgggaga ttcggaacgc gtttatgcgt
     1561 cgatggggaa ctacattttt tccaccggca ggctcctcca cgaactcaaa gaggacgcgg
     1621 agaacgagag cagcagccac gatttcgggc gcgacattct tccctcgctg gttggccagt
     1681 gcgagatgtt cgcctacgat ttcgaccgga accccatccc gggcgatcct ccggaggtgc
     1741 ggccgtactg gcgcgacgtc ggaacgatcg acgcgtactt cgaagcggcg atggacctgc
     1801 gctcggtggt gccggcgcta aacctttaca acaagctatg gccggtgcgg accgcgggct
     1861 atcccgaccc ccccgcgaaa ttcacgttcg acgaagacgg ccggcgaggc gaggcgatcg
     1921 actcgatcgt ctcgggcggg agcatcctgt cgggcggtct ggtgcgccgc tcggtcctac
     1981 accgcggggt ccgggttcac acgggcgcgg tggtggaaga ttcgatcatc tttgacaact
     2041 gcgatatcgg gcggcactgc aagatccggc gagccattat cgacaagaat gtcgccctcc
     2101 cagaggattc cgtaatcggc tacgacctgg aatcggacga gcgcaactac cacgtctcgg
     2161 agggcggcat cgtggttgtc gagggagggc gcactccggt ggacatcgcg agagtgaggt
     2221 tgtagcgcgg gcggcgcggc gggtatccgc gttcctcggc gggttgctct ccagcagaac
     2281 ccgcctcagc ttcatcaacc gcccccgcct ttgtcgtgca attgggaact acggtctttg
     2341 gcccgatcac tcaggtgccc cagtccacct caataggttg ggtctgccgt attcggtcaa
     2401 ggtagtagcg gatcgaatcg ggccgatcaa gaaacctgcc cgccgtcatc tcaccgttga
     2461 attccctctg aacaatcaga atccctcgga cttgattgtc acgtgagtgc atgaagacgt
     2521 acacagccgg gtcgtcgctt gagtacaacg ggatctctgc cgtgcccgac attcccgggt
     2581 agtatcgctt cgcccacatg cgtacaacgg atatcacatc gcattggctt gttgcgattt
     2641 cggatcctac ggcggcttgg ggattgacgg ttatgctctg ctcgtactcg gaaatccgct
     2701 tcggcgcttc tacacagccc ctcgacgctg gaagccggcc tgcctgccca cttgatgcat
     2761 tcggctgtcc gggcgccact tgtacaggaa cgcaacctgg atcatttcgc gggcccagaa
     2821 ataggcgttc gtactcgtaa agcgccgcgg aattcgaccc tctatacaga ggagactgat
     2881 cgacactgac aagaggtccg acggtgccgt ctggattcac ccttgtccca atcgctaccc
     2941 tgaaagcggc aatcaggatc acccatctgt cagtgaaccg cactgctttt gcacgaacgc
     3001 tacccccgaa atgcgccgca ccggatgtgt tccagtagcg gtaccaccaa agcgcgcgat
     3061 tcaccttagt ctccagttga tttgctgagt ctggcgccat cgtcgaattc tgagcacacg
     3121 acacaagcgt gatgacaaac attaccgccg tcgctctcat cgacgcctcc caagtcggcg
     3181 gccggagggc agggggaccg cctgaaccga gcccttggcg gtttgtgcga aatcatcaga
     3241 tcgcttcgta cccttggtcc acgtgattcc gaaaagtatt tccgcgcgcc atttccggtc
     3301 cgccgcttca tgcattgtac gctctctgcg agacgcggca agctcgtctc cagaattgcg
     3361 cattggagca gaaggagatc acaggccaca aaagacgatg gcctgcgcca ccggcttttc
     3421 aggtcagcgg gcagagcgtt tgtggagggc ggagatgacc atgccggtgg cgatgaccac
     3481 gaggcagccg atgacgttgt accagaggaa cgagatgccg gtcaggaatc gcgcggcgaa
     3541 gatcgccgct tcacccgcga tcaccccgta gaac
//
LOCUS       DATIFQ010000342        42785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1645125, whole genome shotgun sequence.
ACCESSION   DATIFQ010000342 DATIFQ010000000
VERSION     DATIFQ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 42785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42785
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645125"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VLE22_15810"
     CDS             <1..381
                     /locus_tag="VLE22_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15923.1"
                     /translation="PGIGDYLISLPERVLRSTSAVAGGLLRELGDAAVPAAVRRTLLY
                     RWSRWTRSAGLASHRTGELFDGVVLDHYTRTLAEIHQTGYLAYWVRALGPYFRAAAAQ
                     FSPSRGSETGRFLRRADRRASDVS"
     gene            492..2657
                     /locus_tag="VLE22_15815"
     CDS             492..2657
                     /locus_tag="VLE22_15815"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HSB15924.1"
                     /translation="MTYSRFLLPVCLVTAAAVAPAQNPSVTVSVDAAANRRPISPEIY
                     GVAHASPSALAELNAPVNRHGGNTTSRYNWQLNADNRGFDWYFESIAYSSSTPGELLD
                     TFIAGSRGAAAEPMLTIPMAGWVAKLGTNRGKLASYSIAKYGAQTDRDWQWFPDAGNG
                     ILTNGQLITWNDPNDASVPANSAFQQGWVQHLTGTWGTAGNGGVRYYILDNEPSIWHS
                     THRDIHPTGATMEEVRDKILNYAAMIKTIDPGALIIGPEEWGWSGYFFSGYDQQYGAE
                     HGWSYLPDRAAHGGWDYLPWLLDQLRQNNQSAGRRLLDVFTVHYYPQGGEFSNDTSSS
                     MQLRRNRSTRSLWDPSYVDETWIADKVMLVPRLRNWVNSYYPGTKIGLTEYNWGAENH
                     INGATTQADIYGIFGREGLDYGARWTTPASSTPTFKAMKMYRNYDGNRSTFGDTSVKA
                     TVPTPDNVSAFAAERSPDGALTVMVISKYLSGNTTVTVSLAGFSSQGVAQPWQLTSAN
                     QISRLADVTFSENTFAIVLPPQSVTLFVIPGAGGNNPPVAVAAASPLSGTAPLAVSFD
                     GDGSSDSDGTIASYAWNFGDGATGSGVTVSHTYNAAGTYTATLTVTDNQGATSSDSVS
                     IAVSSDVPAAPSGLSASVTGTTVNLRWTDNANNEDGFYIERALKTKKPAFSRIASVAA
                     NAATYSNTTVPRGTYLYRAQAFRGGKVSAYSNQVQARVR"
     gene            complement(2691..3560)
                     /locus_tag="VLE22_15820"
     CDS             complement(2691..3560)
                     /locus_tag="VLE22_15820"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB15925.1"
                     /translation="MNRRELLASMPWLAASLGAPVSGAGVKSRFRTAICAYSFRDALK
                     NKSMTYEDLVRLASDLDVDGLDLTVYWFPNTSDEFLMPLRRAAYKMGVDIYSISISTD
                     MCKPPGDTRDAEVAKVKGWVDAAAKLGAGHIRVFGGHVPKGSTEADAATWCADTLKVC
                     AEYSSGKGVILGLENHGGITERAETIIQIVKKVDSPWVGINLDTGNFDRDGYNQIEMC
                     VPYAVNVQVKVDIRVEAGKPEKQDWGRIAAMLAKGGYRGYLALEYEDKEDPASVMPRL
                     MSELTKIARKYTA"
     gene            3625..4566
                     /locus_tag="VLE22_15825"
     CDS             3625..4566
                     /locus_tag="VLE22_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15926.1"
                     /translation="MRSLSILGFILGLLAPAQGWQTGRPPVESYVNIITIDGSMRESI
                     FSASALFEAPNWSPDGKYLLLNSEGRLWRLAVAGGEPETVPTGDVGGVNNDHGISPDG
                     KQIALSAGPIYIMPAAGGDPRRVTDKTPSYYHGWSPDGRTLAYCAEREGNFDIYTIPI
                     EGGQETRLTTQAGYDDGPDYSPDGKWIYFNSDRAGTWDVWRMPASGAGSEDSLAERVT
                     SDELEDWFPHPSPDGKWLLFLSYEKGTKGHPPNQYVSLRIMPLPGAKVNSPKITEVTK
                     LFGGQGTINVPSWAPDSKRFAYVSYRPIRPEESAPRQ"
     gene            4563..5639
                     /locus_tag="VLE22_15830"
     CDS             4563..5639
                     /locus_tag="VLE22_15830"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15927.1"
                     /translation="MIDKPRIRRIVNSVAPIRVCDNGGWSDTWFARYGKVFNIAVYPY
                     VEVQMRVLDCEASDRNRITIHAENYGQRYSIGDPNGLYDKHPLLEAAIDYMRVPAGIG
                     IEVSIFSEAPSGCSTGTSASVSVALIGALDCLTPGRMTPHETAAAAQRIETELLKQQC
                     GIQDQIAAAYGGINYIDMYEYPHASVSPVRVPDPVWWELESRLSLIFVGQSHSSSDVH
                     KMVIRELEDAGPDAPKLAPLRRTAELSKNALYASDFEALGRAMIDNTDAQASLHPGLV
                     GPHHQRIIDVARQYGAIGWKVNGAGGDGGSVTVLSGSDRSSKRAMIREIEAADRCYRH
                     IPIHLSRFGLRTWESTPGPVALAR"
     gene            complement(5608..6258)
                     /locus_tag="VLE22_15835"
     CDS             complement(5608..6258)
                     /locus_tag="VLE22_15835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB15928.1"
                     /translation="MPADREQDLVLEGIVHDLNNVFQTILESVEVLAADEKWTSLARA
                     LRRSASRGQRIVTGLHESAPAVLELEAIVEDAVQFASDVLRVLRIPGIEFACNVEPGI
                     RLPGQPTAWERVLFNLLINAAQAMRSGGAIEIRGRRSANHVEIMVEDNGPGIPPEILP
                     RIFQARFSTRPAGSGLGLHIVKSIVRENGGTVTASNREGRPGASFQITVPMPPVRA"
     gene            complement(6262..7542)
                     /gene="aroA"
                     /locus_tag="VLE22_15840"
     CDS             complement(6262..7542)
                     /gene="aroA"
                     /locus_tag="VLE22_15840"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HSB15929.1"
                     /translation="MEERISPARVLSGSIRLPGDKSVSHRYAMLGAIAEGVTSIRNYS
                     TGADCGSTLACIRALGVPVERDGGQVTIGGRGLDGLKEPAGILDAGNSGSTIRMLSGI
                     LAGQSFATHITGDESLARRPMDRVMKPLAEMGASIRAREGRFPPLEIRGGRLRPIDYK
                     LPVPSAQVKSCVLLAGLFADGTTIVRESVQTRDHTEVALREFGADVEVLSQTITVKGR
                     PKLNGRELTVPSDLSSAAFFLVAAVLVPGSKLVIEGVGLNPTRSALLDFLISMGARIR
                     ILNLSQTAGEPAGDILVENAPVRGGVIEKELTAALIDEIPVLCVLGAASENGLMVRDA
                     AELRVKETDRIATIAENLRRAGVEAEVLPDGLNIPGRQNLRATSFDSFGDHRIAMAFA
                     VAALRAGGECTIKRAEAASVSFPEFYDTLRKIAE"
     gene            complement(7598..7972)
                     /locus_tag="VLE22_15845"
     CDS             complement(7598..7972)
                     /locus_tag="VLE22_15845"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB15930.1"
                     /translation="MSYQEIENPKTYRDEHLQVDFERGRVYLDSLEMVLTRKEYELLA
                     LLVDNTGEIVPRDVLLMQIWGYSREIRTRTLDVHIRRLRKKLGDHGDHYIETIFGVGY
                     RFQRYREPRAFPDFAPVLALTA"
     gene            complement(8061..8453)
                     /locus_tag="VLE22_15850"
     CDS             complement(8061..8453)
                     /locus_tag="VLE22_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15931.1"
                     /translation="MKRALTVLIVLATLLFAVQQKRKPRPPEIEILELNVHRTEELVT
                     LDGRVKNIGEMPAERLVVEFDFRDPDNRVVTTKKGPIDEEILDPDQESAIQFQTKDAP
                     RFVTVSVRAHAARVGDVKVKNAGPFVIE"
     gene            8483..9589
                     /locus_tag="VLE22_15855"
     CDS             8483..9589
                     /locus_tag="VLE22_15855"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HSB15932.1"
                     /translation="MITRRNWMLGAAAVSSATAVQSKPKHIAVASSNGLRACARAVEL
                     LRRGADTLDAAVAGVNINEEDPEDTSVGYGGLPNEEGVVELDASVMHGPTRRCGSVAA
                     LRGVKTPSKVAKLVMEQTDHIMLTGDGALRFAKAFGFREENLLTEKARIAWLVWKQSL
                     RDADGHNNWTDGLDAPASRPSVKPRSSLRMLFPGVDETTLAWARDVVMHPPTGTINCL
                     TLNEKGEMAGVTTTSGLAWKIPGRVGDSPIIGAGVYVDPDVGAAGSTGRGEENIRVVG
                     AHTIVENMRHGMTPKEACLDALRRVARNYDNDAALLAKFDLRFYALRKDGQYAGAALW
                     NGRISGGKFAHAEFSVNSGGASRLEKCAYLLERK"
     gene            complement(9602..10846)
                     /locus_tag="VLE22_15860"
     CDS             complement(9602..10846)
                     /locus_tag="VLE22_15860"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HSB15933.1"
                     /translation="MLKRFRYHIAVFFSVIGPGFITAMVDNDAGGIYTYSQAGAKFGY
                     LPLWTLLPITILLIVTQEMCARMGAVTGKGLSDLIREEFGLRATFLMMAALLLTNLTN
                     IMANFAGIASSLELFHVSRYISVPLGAVAVWLLVVKGTYRSVEKVFLFASALYVSYVI
                     AGILVKPNWAEAAIYSVRPVLMFDTAYITLVIGLVGTSIAPWMQFYLQAAVVEKGVTA
                     KEYVESRIEVIVGCIAMAVIAFFIIVACAGSIWTVSPRDIKDAADAALGLKPFGDYAY
                     LLFCAGLFNASLFAACILPLSTAYTVCEGLGFESGLNQRFREAPVFYWLYTLLVAVGA
                     GVVLLPQLPLVRMILFSQVINGVLLPFVLIFMVLLVNRKGLMGEWVNPRTYNVVAWTA
                     VVVLVGLTLALGAITLRDILAR"
     gene            complement(10859..12100)
                     /locus_tag="VLE22_15865"
     CDS             complement(10859..12100)
                     /locus_tag="VLE22_15865"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSB15934.1"
                     /translation="MAENLLYLTELVGLKVYDLKRHRIGRVKDAALVPLIDSARVDRF
                     LVGGGWAWLTVRHDQVRSISLDGIYLRDELLTPYHSDEYMLRLVRDLLDQQIIDVSGR
                     KVVRVTDVTFEIRKDEDRDVLFVSEVDVGMRSIFRRIVQGLLPRRVVRKLQRPIPPNS
                     IRWEFCNIVEADPQRRLRLNITHRMLEEMHPADLADIVEELSPEDREAIFTAIDSEVA
                     AEALSEVDPKMQASILESLDTEKAADIVEEMSPDQAADVLAELEQETSEEILEEMEPE
                     EKTEVRELLEFEEDTAGGLMNTEYVALHENATVSDALEALKGNEELLETLNTLLLIDG
                     EERLVGVVPLARLFIASGPARLKELATDTPIQVSVDEKQDRVTELFDKYNLLALPVIG
                     EEGKLAGVITADDIITVLRQK"
     gene            complement(12233..13711)
                     /gene="gatB"
                     /locus_tag="VLE22_15870"
     CDS             complement(12233..13711)
                     /gene="gatB"
                     /locus_tag="VLE22_15870"
                     /inference="COORDINATES: protein motif:HMM:NF004015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HSB15935.1"
                     /translation="MSSPTAALVAPEVLAKYEPVIGLEVHVQLATRTKIFCGCPTSFG
                     APPNTNVCPVCLGLPGALPVLSRQAVEMAVKAGLALQCQIRKVSRLARKNYFYPDLPK
                     GYQISQYDEPLAEHGWVEIEVEGVRKRIGVTRVHIEDDAGKSVHEGFRDSDRYSYVDL
                     NRCGTPLIEIVTEPDMRSSDEAYAFLTELKQVMQYVDVSSCDMEKGHLRCDANVSVRL
                     KGAEKLGTKAEVKNLNSFRFLKMALDYEIARQVGVIEGGGRIIQETRLYNPDTGETAG
                     MRSKEHAHDYRYFPEPDLVPLRVSDEWRARIEAEMPELPASRRARFIESYGLREYDAQ
                     VLTATHELSDYFERAAKASGDPRVAANWVMGDLAGALKEQGKEIAESSVSAEHLGELI
                     SLIAKGEISGKLAKEIFAKMLGSGESARVIIDREGLRQISDTTALEKILDDVLAANPK
                     QVEQYRGGKSTVLGFFVGQVMKATRGQANPGTVNELLKQKLQ"
     gene            13774..15180
                     /locus_tag="VLE22_15875"
     CDS             13774..15180
                     /locus_tag="VLE22_15875"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB15936.1"
                     /translation="MLTSSRRQFVSGAAAPFLSPSGRGERRPNVVMFMTDDHGAWALG
                     SYGCPEMDTPNIDRLAAGGARFTRAYACTPVCSPSRMTWLTGRIPSVHGVQDWLIPSD
                     SFGARTRRWLEGHPAYSEVLAKNGYSLGMCGKWHMGDDERPQAGFTYWATVPGGGGPY
                     KDPEFVKKGRRAVIPGYKTDIVGDCALEFLEQVRGNPFFLLVPFYAPHTPFNYQPEEY
                     RQPYLKSRFYCFPDPPAHPWQNPGLKSHHANRASKLAYSALITGADRNVGRIVRKLEE
                     MGVREDTLVIFTADQGWNAGHHGVWGKGNGTVPFNMYEESIRVPLIWNHPRRVRSGQV
                     LPPMVSSYDFFPTILDYLGLSASPDPKRVGRSYAAFLRGGKPRWQNRLYFEFAYVRAV
                     RTENLKYVERTKEWPAELYDLEADPGETRNLIDDPGYRKQRMALHTELSGWFDRMGAP
                     PIEAWRSTTKQELTVYSR"
     gene            15192..16577
                     /locus_tag="VLE22_15880"
     CDS             15192..16577
                     /locus_tag="VLE22_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSB15937.1"
                     /translation="MAKEYDLTPRRVPRVETRYRRIVTEIPAPKSVQILERLHRYEPL
                     AMRGQPPIVWDHAEGFQVWDAWGNCWIDWSSGVLITNAGHGRKEIVEAVAGQANRKLL
                     TTYCFPTETRAQLVERLASLLPEPLKKVFLLTTGSEAVECAIKLCRTHGVKLGGRSKN
                     VIVSYDKAFHGRTLGSQQAGGIPALKEWIVNLDPGFVQMPFPDGFRTEDTSFEYFERS
                     LAAAGVKPEDIAGIILETYQGGSASFAPPAYMQALRKWCDRHKVLLVCDEVQAGFGRT
                     GTMWGFEHYGIVPDLACFGKGISSSLPLSAVAGRPDVMDLHPAGSMTSTHTGNPVCCA
                     AALASIELVVKENLVENARKMGDLLHRKLNELKPRFPQIGWVDGKGLVAGVACVKPGT
                     KDPDGDLAWEVVRRSIEKGVLMFSPVGFGGGTVKIAPPLVINEDAVLESTGVLEEAFA
                     EATVALEAAVR"
     gene            16574..17836
                     /locus_tag="VLE22_15885"
     CDS             16574..17836
                     /locus_tag="VLE22_15885"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB15938.1"
                     /translation="MSKPDVVVIGGGMITNDQILPSLYQMQRSGHIGEIKICALNARP
                     LKDLADSATFKRAFPGQSFRSFPDIAGDLDKPYPDLFREVIASLPPRNVVVVAVPDQL
                     HFNVIMTALKHNQHVCTVKPLVLKAAEALEIEREAYSRGLVVGVEYHKRFDDRSLLAR
                     RRYREGRFGEFRLGTACLLEKWYYRHSNFQNWCTVENSDAFTYIGCHYVDLVHFITGL
                     LPVAVSLYGIRDKYPNGKEGFLWTDARVLWNNGACLNVQNALGFPDEAPGTNTQGMTM
                     YCAGETNGTLISHSDQFRGLKYCYVNRDDSPGATVYAEPSTDYFQYLDLGGPGMIPVG
                     YGYRSVEYIVKAAIRAESETAGLAEADSLAKRQAILKEYDAAGVMATPANSSYNELVI
                     EAGRLSILNGGREAVIEYGKKPGVRLRN"
     gene            17838..19226
                     /locus_tag="VLE22_15890"
     CDS             17838..19226
                     /locus_tag="VLE22_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HSB15939.1"
                     /translation="MHNRRLQLSREEMRALGYRIVDRLVEHLDNQTSQRVATKGVRAE
                     LESRLRESLPEEPASPDDVLKQLEEDVFSHMLHVDHPRFFAFVPSPGNYVGALADALA
                     SGMNPFVGTWLGASGPAQVELVTIDWLRQICGLPDGSGGIFVTGGSVANLIALAAARH
                     ATLGEDTAHAIAYFSDQTHFAVERGLRVLGFAPAQLRRLPSDDRFRLVPAELERAIRE
                     DRLAGRRPFCVIANAGTTNTGAVDPLPVLREICSRESLWLHADGAYGAAGMLSRKGRA
                     LLDGLPQVDSLALDPHKWLFQPFEIGCVLVRDAGLLRSTFQVRPEYLKDALPTEDEVN
                     LSDYGVQLTRSFRALKLWMSLKVFGLSAFREAVDWGIRLAEIAEGRLRSSGKWEIVSP
                     AQLAIVAFRYAGGDDAVNLRIIDRMYDDGFAFLSSTILCERPALRLCTINPRTTEADI
                     LETIDRLERFAQ"
     gene            19560..19919
                     /locus_tag="VLE22_15895"
     CDS             19560..19919
                     /locus_tag="VLE22_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15940.1"
                     /translation="MRRLLLVLLVAALPSPGMSPKTKRRLTFAAVAAAQMLDLHTSLG
                     RKEGNPLLRGPNGRFAAGRGVGVKLGILSGLLAAQELRPAPYWNWVNVTYAGASATIA
                     VRNYRVSKPPEYLGPAR"
     gene            complement(19940..20236)
                     /locus_tag="VLE22_15900"
     CDS             complement(19940..20236)
                     /locus_tag="VLE22_15900"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HSB15941.1"
                     /translation="MTPAAQPKIQLSFSQDYREGYANSVQVRANLWDFLLLFGTINQT
                     APDSVNIRNFQGVYLSPQQAKALANLLQQNIQQYEGAFGEIRLEPQGGGGGVVQ"
     gene            complement(20240..20812)
                     /locus_tag="VLE22_15905"
     CDS             complement(20240..20812)
                     /locus_tag="VLE22_15905"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HSB15942.1"
                     /translation="MVTASQLRAGMAVRYEGQAYKVLIADYHPGQGKMGGATHARLRN
                     LSTGTLWEHSFRSDLKLEDLPVEKQSMEYLYNDADACYFMNPETFEQVPIPVAIIGPQ
                     ARFLRPEMLVPVEFIEGQPVSVLFPDIIEVRIADTAPPVHAQQDSTWKPSVLENGVEI
                     MVPQFIKVGDLIRLDVENLKYIDRAKGGGK"
     gene            complement(20959..21035)
                     /locus_tag="VLE22_15910"
     tRNA            complement(20959..21035)
                     /locus_tag="VLE22_15910"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20999..21001),aa:Asp,seq:gtc)
     gene            21113..22087
                     /locus_tag="VLE22_15915"
     CDS             21113..22087
                     /locus_tag="VLE22_15915"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSB15943.1"
                     /translation="MKPACLIPAMFAILGVRASGEVVRVDPALDQIVSEAAQIEKVAG
                     GFAFTEGPIWARDGYLLFSDIPNNVIQKWTPEGKVSLFRKPSGYDKTDAPKGAFIGSN
                     GLTLDREGRLTICEHGNGRVTRIEKDGKLTVLAAKYEGKRLNSPNDAVYKSNGSLYFT
                     DPPYGLPKEDQDPKKELPFNGIYRIADGVLQLLSKELTRPNGLAFSPDEKYLYVANSD
                     PARKIWMRFEVKLDGTISKGRVFYDVTRRTEEGLPDGMKVDRRGNLYCTGPGGVWIFS
                     PTAKHLGTIRLPETPANCAWGDADGKTLYTTARTSLYRIRLNVEGVRP"
     gene            22126..22470
                     /locus_tag="VLE22_15920"
     CDS             22126..22470
                     /locus_tag="VLE22_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15944.1"
                     /translation="MGTYRGWPCLLTLVFTAASAGADSPWVETRTFYTNAEGLPDTEV
                     LAVAATSGGDVYVGAPKGLARLSRGQWVTVPALAGTRVELLVPDGPDLLVATSDGLHR
                     LSAKAATRFVKP"
     gene            complement(22478..22996)
                     /locus_tag="VLE22_15925"
     CDS             complement(22478..22996)
                     /locus_tag="VLE22_15925"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSB15945.1"
                     /translation="MSMWNKRASDDDHPRAEQRRPASPAEVMQAPPPPPARSAADTQR
                     RPAAIGQSMVVKGEILSREDLSIDGEMEGKLDVGQNRLTIGPNAKIRASIRAREVEIY
                     GAVHGNVEASDRIILRKNSRLTGDLKMASVVIEDGAFFKGSIDITQPQTAKTAAAPAP
                     GQPSSVSASTSG"
     gene            23360..23974
                     /locus_tag="VLE22_15930"
     CDS             23360..23974
                     /locus_tag="VLE22_15930"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HSB15946.1"
                     /translation="MRLIPRDEKFFQLFLKQVRLISQASDVLLTGVKAGNAKMATACE
                     VIQRLEHDGDDVIHDILTRLNQTFITPLDPEDIHSLSSSLDDVLDGIEDAAYQLTAHN
                     VEPIPPAVVELCEIVNYCSHSIALAFQALHDGKALLEHCVEINRLEGVADLLVRRVLA
                     ELYRSDIHPMAFIKLKEVYEVLEATTDRCEDVADALQGVVVKNS"
     gene            23990..25009
                     /locus_tag="VLE22_15935"
     CDS             23990..25009
                     /locus_tag="VLE22_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HSB15947.1"
                     /translation="MLSLVVIIVAAALIFDYINGFHDAANSVATVVATRVLSPFQAVL
                     WAGFFNFASVFSFGTGVAHTVGAGMVDMTMVTPYVILSGLIGAIIWDLFTWWLGLPTS
                     SSHALFGGYAGAAMAHVAHLKGVAHAADVILWKGWIGTLFFMVASPIIGLVLAYLLMV
                     CIYWLFRRSTPRKMDVYFRKLQLFSAAVYSYAHGTNDAQKTMGIITLVLVTGGYLEKF
                     EVPVWVMLSACGAIALGTLTGGWRIVRTMGVRLTRLRPRGGFCAEAAAATSILLATHL
                     HIGVSTTHVITGAIAGVGSIRRVKAVRWGVAANIIWAWIFTIPAAALVAACCFALIRV
                     FVRES"
     gene            complement(25080..25168)
                     /locus_tag="VLE22_15940"
     tRNA            complement(25080..25168)
                     /locus_tag="VLE22_15940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25132..25134),aa:Leu,seq:taa)
     gene            25496..25837
                     /locus_tag="VLE22_15945"
     CDS             25496..25837
                     /locus_tag="VLE22_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15948.1"
                     /translation="MSRIVSLLVFSTVFSMLALAESWSGKLLDANCYDQQKQASACSA
                     TSETTAFALDAAGKIYKLDAAGNSKASDALKNRADRATDPAKPQSKEVMAQVTGAEKD
                     GTIAVESIEVR"
     gene            26067..26465
                     /locus_tag="VLE22_15950"
     CDS             26067..26465
                     /locus_tag="VLE22_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15949.1"
                     /translation="MAPVTPREPDVRERERLAETFLREKLALWQRRLHLQDWTISVVT
                     SNSDALRPGTRGNIRWDADTKTAAIRVLDASEYRMPFRAALKDMEFTIVHELIHLTLS
                     PLPRSESSRSKEEYAINRIADALLQGDRDD"
     gene            26641..27783
                     /gene="phoU"
                     /locus_tag="VLE22_15955"
     CDS             26641..27783
                     /gene="phoU"
                     /locus_tag="VLE22_15955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HSB15950.1"
                     /translation="MDASPAPHFEALLQRDIDVIRNRLLEMLRLDEEALKRAFDAVLS
                     RDRQLAYSVILRDQEVDSRETELDRLCIEFIMRHQPVASHLRFVYGASKVISQLERVG
                     DYAESIARQALLLSSVSYEVPREMYSEIAKLAVSMLHNAVRAFVDKNPDLARATMLSE
                     PRVNQVRDRIIVEVLQWREQGILPLDALAPMMTVARRFERVSDQATNICEEALYFATG
                     EYMRHFPSGGFRVVFVDETHGCLSRMAELIGNRLGAERFSFSSAGVEAGTLDPQTVWF
                     LAEKGFDASHEKSLSIEQIPQLEHVQMVILLSKEAEAVIPRAPSKALNVRWLVADPTR
                     VHGTPDEIRAAYEHAFEALTTHIRDLIQAVLGNHGNPSDENTNSTI"
     gene            27794..28681
                     /locus_tag="VLE22_15960"
     CDS             27794..28681
                     /locus_tag="VLE22_15960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSB15951.1"
                     /translation="MRFASCALAAVALAFLAVGCARSKQPEKTVIQNTGSDTMVNLAQ
                     AWAEEYAAVNPTVSVEVSGGGSGTGIAALINGTVDIANCSRRVELQEIEQAKSNTKKE
                     PREFMVGYDAMAVYIHKSNPLDEITLDQLAEIYREGGKITRWSQLGAKMPPEADEIIL
                     LSRQSNSGTYYYFREAVLGKGHDFKLGSRDMHGSKDVVELVSKTPAAIGYSGMGYATP
                     DVKMLRVAKKAGDKAYAPTVENTLNHTYPIARPLFMYTLGEPSGAVKTYLDWIHSEAG
                     QKIVVQSGYVPLSQPGAPR"
     gene            28685..29599
                     /gene="pstC"
                     /locus_tag="VLE22_15965"
     CDS             28685..29599
                     /gene="pstC"
                     /locus_tag="VLE22_15965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HSB15952.1"
                     /translation="MSTAGSSTPIRRRWQAGTETAIEALIHVCGISAIVFVFGIFFFV
                     FREGAGILFRSFSFREFFTSTEWYPTSQSHVRYGVLALIAGTASVTALAMALAVPFGI
                     GAAVFISEFCGPRLRESLKIVIELLAAIPSVVWGFIGLTVMNQLIMAVFDAPIGLNVL
                     NGGVILALMSVPIIVSIGEDALKAVPDSYREAALALGVTRWQLVYRVLLPAAKNGLLA
                     AVLLGVGRAVGETMAVLMATGHAVKVPTSLMDSVRTLTATIAAELGEAPVNSDHYQVL
                     FVIGILLFMITFVVNLTADLVVRGIRNK"
     gene            29629..30513
                     /gene="pstA"
                     /locus_tag="VLE22_15970"
     CDS             29629..30513
                     /gene="pstA"
                     /locus_tag="VLE22_15970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HSB15953.1"
                     /translation="MFQATPFTRRKQASELLAKSIFLLMTLLMVLPLILIVGYLVYQA
                     WPILSLDFLLTNPRHGMRAGGIWSPLLGTIYLVVISLIIATPIGVLAAVFLNEYARDT
                     WFTRMVNLAVVNLAGVPSIVHALFGLGAFVLAAGLGKSILAASLTLAIMTLPVIIAST
                     KEALAAVPMAFREACWNVGASRWQTIRHVVLPNSISGILTGVILQVSRAAGETAPVMF
                     TGVVFFKAIREGDLFAYGPFDQCMALSMHLFTISTQVPDVPKALPYGTAVVLLGTVLA
                     VNALAILIRVRLRSRRKW"
     gene            30507..31271
                     /locus_tag="VLE22_15975"
     CDS             30507..31271
                     /locus_tag="VLE22_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HSB15954.1"
                     /translation="MVTPGPMVELADLRVRYGQREALKGVTFEMRRNEVFGIIGPSQS
                     GKTTLLKVINRTLDRVPGVMISGTVMVDGVNIHRMKDVYSLRRKVGMVAPLPVGLPLS
                     IFDNVAFAPRMAGIRDKADLEARVERCLRQAALWDEVKDRLDSLGTRLSGGQQQRLTI
                     ARALSHDPEVLCLDEFSIAVDPVTTMRIEDVLKELRSRMTIILVTNLVQQARRLADRT
                     AFFLNGELVETGPSEVIFSDAPANRKTYEYVNGIFG"
     gene            31268..32053
                     /locus_tag="VLE22_15980"
     CDS             31268..32053
                     /locus_tag="VLE22_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HSB15955.1"
                     /translation="MTPPAAATSFSIETRKLSLWYGSFQALKDVSITIRQGEITGLIG
                     PSGCGKTTLLRCFNRINERYGYVRTTGEIRILGKNIYDTDVSLIELRKEVGMVFQRPN
                     PLPISVYDNVVFGLRVHSGLGSLRKSVLDEAVEKALREVSLWDDLKDRLGVRATTLQL
                     DQQQKLCIARLLPLKPEIILMDEPCSALDSEATLGIEELMLSLAGRYTILIVTHNMAQ
                     ARRVSDECAFMLLGEIVEHARTEDLYLTPRDPRTADYIEGRYG"
     gene            32060..33208
                     /locus_tag="VLE22_15985"
     CDS             32060..33208
                     /locus_tag="VLE22_15985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB15956.1"
                     /translation="MNERDHSGPREAPAPVLLMVRELGPGGSERQLAVIARALDREQF
                     EPHVGCFRPDGIRGDELRAAGIPILEVPVHSFASTRAFGAVRPFARYIHERGIRLVHT
                     FDEPMNVWGVPAARVAGVGVVLSSQRAHRELVPPLRRHMLRFTDHLTDGVVVNCLAVR
                     RHLIEDEKVSPSLIHLCYNGIDTAAFAPERRRPDSLAAAGLTIGCVCALRPEKDLTTL
                     ARAFAAVRHLHPDMRLVIVGSGPSLPLLRDCCHSLDLENQCVFEPATSRVAEWLGGID
                     IFVLPSLSEALSNALMEAMACGCSPVATRVGGNPELVEHGRTGFLFSPGEPEELAAVL
                     RTLIENDPLRRELACAASAKVRDNFSIEVAARRMGEIYRHFLDRRGDR"
     gene            33252..34517
                     /locus_tag="VLE22_15990"
     CDS             33252..34517
                     /locus_tag="VLE22_15990"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HSB15957.1"
                     /translation="MNELRGINRRRALTALGAVPIAGLAPQTRAAEDGAAAPSDKPRE
                     LAPMEADMGTLYPDIERLVAANRPSHSFLDSRFQSLEDWKRRGRELAWESFGYRPATV
                     DLNPKVVDRQDLGEFVREKVLFSTTPQFRVAAYVHIPKNRTGRGPAIVDLHSHGGMFL
                     FGKEKVIDFGRNHPAMTEYHKGNYGGRPTTTALVRRGYVVVTIDAFMFGERRLIMDED
                     LKYGWERSRYSVEDVKHLNARCASKEDTLAKSLTLAGIAWNGVVAWDDMRTVDYLLTR
                     PEVDPKRIGCVGVSFGGYRTLFLAGLDERIAAACVVGFMSTIKPMARRHIDTHSWVHF
                     VPSLHRYMDWPDVVGMMAPKPLMVQQCRQDGLFPLTGMRESLERIAAIYEKAGVKSHF
                     DGRFYDGGHRFDVPMQEDAFDWFDRHLKA"
     gene            34636..36498
                     /locus_tag="VLE22_15995"
     CDS             34636..36498
                     /locus_tag="VLE22_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15958.1"
                     /translation="MTSRFAAIAFVFAAVAPVCPSQEVAPPRPRAGKVEILKSAALKP
                     GMKGVAWTVFQGSEPEPVPVEIIGLWKNAWGPRQDVILAKLGGKVQRTNVAGGMSGSP
                     VYIDGKLIGAIALRLSVFSPDAICGITPIELMLEISDFDSTVPSGARTPDKAVVRDPL
                     AVPGEVLAQAVAAGASPGLLQQSPLMVPIETPLSFSGFHENVLREFGPLFQQLGLTAV
                     QGGASGSLGGPKPAPGWQNALQPGDPVAGVLVSGDMTISGLGTVTYNDGKRVLAFGHP
                     FFNLGPVEMPLSKGEVLMVLSSSFQPNKIANATEVVGSLKQDRHSGILGVLGETADMI
                     PVSVKVRSFAGDNSVLKEKAFRYNVFVQQRWTPNLMMITLFNSISGVNDFSDENTYRL
                     SGKVELDGGQSLSLETMQAPSEVPIPAPILLAGWVGDKFNRLFLNAVKAPRLKRVDVN
                     VDLLPQRRIAAIESAWAGLSEVDAGGEVPVKVFLRPYRGERIERAFNLKIPAGLPKGE
                     HGILLSDAETLNRLKGAVGRANRFIDLAQTVSLINQERTNNQLYVSLVQASPTVYYDD
                     KTLPSLPASVLNVMQAGNAANRPFITSRETVLEQTAIPFDYVIDGSYALKIKVK"
     gene            36509..38710
                     /locus_tag="VLE22_16000"
     CDS             36509..38710
                     /locus_tag="VLE22_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15959.1"
                     /translation="MQRIRFFRTVAAAGAICCLAGAVETRSWQQDAYEDFDKGHIAKI
                     SLRSDGQLMLAPVFKELLDSPSPYLWALAEDSKGNLFAGGGGPGGAGAQVYAISPDGK
                     SRTLAKLEGLEVHALAVDKRDRLYAATSPDGKIYRISSGGAAETVYSPGAKYIWAIAF
                     DSKGNLFAATGDEGQIHRVTPDGKGAVFYRTEETHARSLAVDENDNLIVGTDPGGLIL
                     RITPAGEGFVLHQAPKREITAVAVAKNGSIYAAATGVKQPTMAPPPPLPKPVVSPVKP
                     AGPPSPGQVQPSPTPPANLPSVATAAEGSEVYRIDPDGYPRRVWSHSKDLVYAIVFDS
                     SERPLVATGNKGIVYRFDSDHVYTALVSVQPTQVTALCAGLKGKVYAATGNIGKVFQI
                     GPGLEKEGFVESDVLDAEIFSLWGRLAYRGDLNGGDIRVETRSGNLDRPRKDWSPWQP
                     VGLGEDGGRIQSPQARFLQWKLTLTSASDGHSPEIRSVDVAYLPKNVAPVVEQIEITP
                     PNYRFPPQSLSLTQTQTLTLQPLGRARRGSPPPGLIDSGVLSMHYAKGYIGARWTADD
                     ENGDEMLYDVAIRGASETDWKLLKEKIKEKYLSWDSTAFPDGEYWLRVTASDTPDNPP
                     DQALSAQLVSERFAIDNSPPEISGLAASQSGGKLEVHWKARDVLSVIGKAEYSVNGGE
                     WTFVEPVTKLSDSKELDYRLVLDKLSPGEKTIAVRVADEFDNLAVAKAVVK"
     gene            complement(38722..39654)
                     /locus_tag="VLE22_16005"
     CDS             complement(38722..39654)
                     /locus_tag="VLE22_16005"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSB15960.1"
                     /translation="MKANLLPLLFLFLLEPATAQNVVREVRTAIARDDFAGAEVLVRR
                     YRAEKGDTPEALAALSWLGRGALEAKKTDEAEVHAAEVLRVGESLLKSRALDTDRYLP
                     VALGAAIETQAQVMAARGERSGAVEFLNRALDRYRGTSIRTRIQKNIHLLSLEGKSAP
                     PLETAVWLGPKPESFDQLKGRPVLLFFWAHWCGDCKGQAPVLARLIKEYGPRGLALVG
                     PTQRYGYVARGMEVTPEVELNYIDDVRRRFYAELSEMPVPVGEESFRNYGASTTPTLV
                     LLDRHGIVRLYHPGEMSYQELAPKVEAIVGKPSS"
     gene            complement(39651..40685)
                     /locus_tag="VLE22_16010"
     CDS             complement(39651..40685)
                     /locus_tag="VLE22_16010"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB15961.1"
                     /translation="MDRRSFLAATGVAMAAQPSFQGKPVVKFGIDLFSIRSQGWSPFE
                     YLDYCAKWKANVVHFSEIRFLGSLEESHLKKVRAHADRLGIELEIGMRSICPTSKAFD
                     PAQGTAEEQVMRMVKAAQTTGARLVRAFLGTMADRTGSIPIEGHIENTVRVLKAVRSR
                     VLDAGVRIAVENHAGDMQARELRMLIEEAGKDFVGACIDSGNPLWTLEDPHLTLEVLH
                     PYSVTSHVRDSAIWRVPEGVAVKWVRMGEGNVDLDGYVRKFLELCPGRALSLEVLVTQ
                     PRVFSYLDPEFWEGYRNVRAWEFSRFLALAERGKPHEGLPPAPKEEAAQREREDLEAS
                     IRHCQKLLMI"
     gene            complement(40703..41587)
                     /gene="galU"
                     /locus_tag="VLE22_16015"
     CDS             complement(40703..41587)
                     /gene="galU"
                     /locus_tag="VLE22_16015"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HSB15962.1"
                     /translation="MVPKVRKAVFPAAGLGTRFLPATKAQPKEMLPLVDKPLIQYGVE
                     EALRSGIQNIIIVTGRGKSAIEDHFDVSFELEHLLESRNKTETLAIVRDISDMINVAY
                     VRQKEALGLGHAVLRARELVGREPLAVVLSDDVIYSEVPCLRQLLDVYEFFGASVLAL
                     MEVPSDNISAYGVVEAEPVSHNGEEDRVFRIRNLVEKPKPSEAPSNLAIIGRYVLTPE
                     IFDSIEAIEPGSGGEIQLTDALKHLLCSRPIYGYRFQGARYDAGDKLGFLKATVEFAL
                     RRKDLGGEFREYLKGLEL"
     gene            41695..41955
                     /locus_tag="VLE22_16020"
     CDS             41695..41955
                     /locus_tag="VLE22_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HSB15963.1"
                     /translation="MAYVIAEPCIGTKDTACVDACPVDCIHPKKDEANFEKEEMLYID
                     PVECIDCGACVPVCPVSAIFALDDLPEKWAEFTARNAAYYGR"
     gene            complement(41943..42323)
                     /gene="queD"
                     /locus_tag="VLE22_16025"
     CDS             complement(41943..42323)
                     /gene="queD"
                     /locus_tag="VLE22_16025"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HSB15964.1"
                     /translation="MFEISVEETFAAGHALRGYRGKCENLHGHNYRVLVTIEGEELNS
                     IGLLVDFVDVKRVLHAAVERLDHQFLNEVPPFDVQNPSAENMAKYFYDELSKGLASQE
                     PATPVRVAAVKLWETDTSTATYRP"
     gene            complement(42316..>42785)
                     /locus_tag="VLE22_16030"
     CDS             complement(42316..>42785)
                     /locus_tag="VLE22_16030"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HSB15965.1"
                     /translation="RVVYDRISFDTEYPTLLQGTGPRTALLDEFRATPHCVLFATASF
                     WQGVDVPGEQLSCVIIDKLPFAVPSDPVVEARIRSIREEGGNPFYDYQIPQAALSLKQ
                     GFGRLIRSRTDRGVLALLDNRITRQRYGQVFFDSLPDYRFTTDLSEVEKFFGV"
BASE COUNT         8249 a        13312 c        13031 g         8193 t
ORIGIN      
        1 ccaggtatcg gtgactacct catatcgcta ccggagcggg ttctccggtc gacgtcagcc
       61 gtagccgggg gcttgcttcg cgaactcggc gatgctgccg tccctgccgc ggtgcgccgg
      121 acgctcctct accggtggag ccgctggacc cgctcggccg gcctggcatc ccaccgcacc
      181 ggagagttgt tcgatggcgt ggtgctcgat cactacactc gaacacttgc cgagatccat
      241 cagaccggct atctcgccta ctgggtgcgc gcgctgggtc cgtacttccg tgccgccgcc
      301 gcacagtttt cgccgtcccg cggctctgaa accggacgat tcctgcggcg cgctgacaga
      361 agggcgagcg acgtaagtta acggtacgcc gtttacccgg tttacttcct tcctagtact
      421 taccgttcgg aattcccatg gaattccagt gaccaccgat gctacgttcg ggcggaagga
      481 cagcactcca catgacctac agccgttttc ttctgcccgt ttgcctagtc accgccgctg
      541 ccgttgcgcc ggcgcagaac ccgagcgtaa ccgtgagcgt cgacgctgcc gccaaccggc
      601 gtcccatcag cccggagatt tatggagttg cccacgcgtc tccgtctgcg ctggccgagc
      661 tgaacgctcc ggtgaaccgg cacggaggta ataccaccag ccgttataac tggcaactca
      721 acgccgacaa cagagggttc gactggtact tcgagagcat cgcgtattcg agttcgacac
      781 cgggggaact gctggatacg ttcattgccg gcagccgcgg agccgccgct gaaccgatgc
      841 tgacgattcc gatggccggt tgggtggcga agctggggac gaaccgcggc aaacttgcga
      901 gctattcgat cgcgaaatac ggagcgcaga cggaccgcga ctggcagtgg ttccccgacg
      961 ccggcaacgg aatcctgacc aacggccagc tcatcacttg gaacgatcca aacgacgcga
     1021 gcgtgcccgc caattcggcg ttccagcagg gatgggtcca gcacctgacg ggcacgtggg
     1081 gcacggccgg gaacggcggc gtccggtatt acatcctaga caacgagcca agcatctggc
     1141 attcgaccca ccgcgatatt catcccaccg gagccaccat ggaggaggtc cgggacaaga
     1201 tcctcaacta cgccgcgatg atcaagacca tcgatcccgg cgcactcatc attggcccgg
     1261 aggaatgggg ttggagcggg tacttcttca gcggatacga ccaacagtac ggcgccgaac
     1321 atggatggag ctacctgccg gaccgggccg ctcacggcgg ctgggactat ctcccgtggc
     1381 tgctcgacca gttgcggcag aacaaccagt ccgccgggcg ccgcctgctc gatgtcttta
     1441 cggtgcacta ctacccgcag ggaggcgagt tcagcaacga cacttccagt tcgatgcaat
     1501 tgcgccggaa ccgctccaca cgctcgctct gggatccgag ctacgttgat gagacgtgga
     1561 tcgccgacaa ggtcatgctc gtccctcgcc tgcgaaattg ggtgaacagc tactacccag
     1621 gcacgaaaat cggcctgacg gagtacaact ggggcgcgga gaaccacatc aacggagcca
     1681 ccacccaggc cgacatctat ggcatcttcg gccgggaggg actggactac ggggcccgct
     1741 ggaccacgcc tgcttcatcg actcccacct tcaaagcgat gaagatgtac cgcaactacg
     1801 acggtaacag atccacattt ggcgatacga gtgtaaaggc aaccgtgccg accccggaca
     1861 acgtctccgc cttcgctgcc gagcggtcgc cggatggcgc cctgacggta atggtgatca
     1921 gcaagtatct gtccgggaac acgactgtca ccgtaagcct ggctggcttc agcagccaag
     1981 gggttgctca gccgtggcag cttacctcgg cgaatcagat cagccggctg gcggatgtca
     2041 ccttcagcga gaacacgttt gcgatcgtgc tgccgccaca gagcgtgacg ctatttgtga
     2101 ttccgggtgc aggcggcaac aatccgccgg ttgcggtggc tgcggcgtcg ccgctctcgg
     2161 gaactgcgcc gctcgccgtg agcttcgatg gcgacgggtc ttcggactcc gacggaacca
     2221 tcgcctccta cgcctggaac ttcggtgacg gcgcgacggg atcgggcgtc acggtgagcc
     2281 acacatacaa tgctgccgga acttacaccg cgactctcac ggttaccgac aaccagggag
     2341 ccacttcgag cgattccgtg tccattgctg tgagctcaga cgttccggcc gccccatccg
     2401 ggctttcggc aagcgtcacg ggcacgactg tgaacctgcg gtggactgac aacgcgaaca
     2461 atgaggacgg gttctacatc gagagagcgc tgaaaacgaa gaaacctgcc ttcagccgta
     2521 tcgcttcggt agccgcaaat gcggccacat attcaaacac gactgtccct cggggaacgt
     2581 atctctatcg ggcacaggcc ttccgcggcg gtaaggtctc ggcctactcc aatcaggtgc
     2641 aggcccgcgt ccggtgagcg gggtgcacat ccccccatcg ccggtaattc tcaggctgtg
     2701 tactttctcg cgatctttgt gagctcagac atcaggcgcg gcatcaccga cgccgggtcc
     2761 tctttgtctt cgtattcgag cgccaagtat ccgcggtagc cgcctttggc gagcatggca
     2821 gcaatcctgc cccaatcttg tttctctggc ttcccagcct ccacacggat atcgactttc
     2881 acctggacat tgaccgcgta cgggacgcac atctcgatct ggttgtagcc gtccctgtcg
     2941 aagttcccgg tgtcgagatt gatgccgacc cacggagagt cgactttctt cacgatctgg
     3001 atgatggtct cggccctttc ggtgatgccg ccgtgatttt cgagccccag aatgactccc
     3061 ttgccgctgg agtattcggc acagactttc agcgtatcgg cacaccatgt tgccgcatct
     3121 gcctccgtgg agcctttcgg aacgtggcct ccgaacacgc ggatgtgccc cgcaccgagt
     3181 ttcgccgccg cgtcgaccca gcccttcact ttcgcgacct ccgcgtcacg ggtgtcgccg
     3241 ggcggcttgc acatatcggt gctgatggag atggaataaa tgtccacgcc catcttgtag
     3301 gcggcgcggc gcagcggcat caggaactcg tcagaggtgt ttggaaacca atacaccgtc
     3361 aggtccagtc cgtctacgtc gaggtccgac gccagccgca cgaggtcctc gtacgtcatc
     3421 gatttgtttt tgagagcgtc gcggaacgaa taggcacaaa tagcggtccg aaatcgggac
     3481 ttcacacccg ctccggacac cggcgcgccc agcgaagccg caagccacgg catactggcc
     3541 aaaagttctc ttcggttcat accatctcct cgaaatatcc tacaacgcat ccccttgcga
     3601 atgcgtatcg gcgataatct gcctatgcgt tccctgtcga tcttgggctt tatcctcggc
     3661 cttctggcgc cggcacaagg ctggcagacc ggacggcccc ccgtcgaaag ctatgtcaac
     3721 atcatcacca tcgatggctc catgcgagag agcatcttct cggcctcggc tctgtttgag
     3781 gcgccgaact ggtcgcccga cggcaagtac ctgctgttga actcggaggg gaggctgtgg
     3841 aggctggccg ttgccggcgg cgagccggag acggtcccga ctggggacgt cggtggggtc
     3901 aacaacgacc acggtatctc gccggatggg aaacagatcg ccctctccgc ggggccgatc
     3961 tacatcatgc ccgcggccgg aggcgatccc cgccgggtga ccgacaaaac gcctagctac
     4021 taccacggct ggtcgcccga cgggcgcacg ctagcgtact gcgcggagcg ggaaggcaac
     4081 ttcgatatct acacgatccc catagaaggt gggcaagaga cgcgcttgac gacgcaggcc
     4141 ggatacgatg acggacccga ctactctccc gacggcaagt ggatctattt caactccgac
     4201 cgggcgggga catgggacgt ctggcgcatg ccggcctccg gcgccggctc cgaagactca
     4261 ctcgccgagc gcgtcacgag cgatgaactc gaagactggt tcccgcatcc gtcgcccgac
     4321 gggaaatggc tcttgtttct ctcctacgag aagggaacca agggtcatcc accgaaccaa
     4381 tatgtgtcgc tgcgcatcat gccgctcccg ggcgcgaagg tcaacagtcc gaagatcacc
     4441 gaggtaacga agctgttcgg ggggcaaggg acgatcaatg tcccctcgtg ggcgcccgac
     4501 agcaagcgtt tcgcctacgt cagctatcgc cccatccgcc ctgaagagag cgctccgaga
     4561 caatgatcga caaaccgagg attcgccgca tcgtcaacag cgtcgcgccc atccgtgtgt
     4621 gcgataacgg cggctggagc gacacctggt tcgcccgcta cggcaaggtc ttcaacatcg
     4681 ccgtctatcc gtacgtagag gtacagatgc gggtgctcga ctgcgaggcc agcgaccgca
     4741 accgcatcac gatccacgcg gaaaactacg gccagcgtta ctccatcggc gacccgaatg
     4801 gtctctacga caagcacccg ctgctggaag ccgccatcga ctacatgcgg gtacccgccg
     4861 gcatcgggat cgaggtttcg atcttcagcg aggcgccgag cggttgctca accggcactt
     4921 ccgcctctgt cagtgtcgct ctgatcgggg cactggactg cctcactcca ggccgcatga
     4981 ccccacacga aactgccgcc gcggcgcaga gaatcgagac ggaactgctc aagcaacagt
     5041 gcggcatcca ggaccagatc gccgcggcgt acggaggcat caactatatc gacatgtacg
     5101 aatacccgca cgcctctgtc agcccggttc gcgtgcccga tccggtctgg tgggaactgg
     5161 agagccggct gtcgctgatc ttcgtcggcc agtcgcacag ttcgtcggac gtgcacaaaa
     5221 tggtgatccg ggaacttgag gatgcaggtc ccgatgcccc gaaactcgca cccctacgcc
     5281 gcacggcgga actgtccaag aacgcgcttt acgcaagcga cttcgaggcg cttggccggg
     5341 ccatgatcga caataccgac gcccaggcat cgctgcatcc gggcctggtc gggccacacc
     5401 atcaacgcat cattgacgtc gcccgccagt acggtgcgat cggttggaaa gtgaacggag
     5461 ccgggggaga cgggggatcg gtcaccgtcc tgagcggctc ggaccggtct tcaaaacggg
     5521 cgatgatccg cgagatcgag gccgcggacc ggtgttaccg gcatattccg atccacctga
     5581 gccgcttcgg gcttcgaacg tgggaatcta cgcccggacc ggtggcattg gcacggtgat
     5641 ctggaagctc gctccgggtc gcccttcacg attggaggcg gtcacggtgc cgccgttttc
     5701 ccggacgatc gatttcacaa tatggagacc gagtccggac cccgcgggcc gcgtggagaa
     5761 ccgcgcctgg aaaatccgcg gcaggatttc ggggggaatt cccggcccgt tgtcctccac
     5821 catgatctcg acatggttgg cgctccggcg gccgcgaatc tcaatggcgc cgccgctccg
     5881 catcgcctgc gcggcattga tcagaaggtt gaacaggacc cgttcccagg cggtcggttg
     5941 tccggggagc cggatacccg gctccacatt gcaggcgaac tcgatcccgg ggatgcgcag
     6001 aacgcggaga acgtcgctcg caaactgcac ggcgtcctcc actatcgcct cgagttccaa
     6061 cacggctggg gcgctctcgt gaaggccggt gacaatccgt tgacctcgcg acgcacttcg
     6121 ccgcagagcg cgtgccagtg acgtccattt ttcgtcggcg gccagcactt cgacggactc
     6181 gaggatcgtc tggaagacgt tgttcaagtc gtgtacgatg ccctcgagga ctaaatcctg
     6241 ctcgcgatcc gcaggcacgg actactccgc aatcttccgc agcgtatcgt aaaactcggg
     6301 aaacgatacg gacgccgcct cggcgcgctt gattgtacac tcgccgccgg cgcgcagcgc
     6361 cgcaacagca aacgccatcg cgattcggtg gtccccaaag gaatcgaacg aggtggctcg
     6421 gaggttctgc cggccgggga tgttcagccc gtcgggcagg acctcggcct ccactccggc
     6481 ccgccggagg ttctcggcta ttgtggcgat ccggtcggtc tccttcaccc gcagttcggc
     6541 cgcgtcgcgg accatgagac cgttctcgct ggcggccccc aggacgcaga gaaccgggat
     6601 ttcgtcgatc aacgccgctg taagctcttt ttcgattacc cctccgcgga ccggagcatt
     6661 ttcgaccagg atgtcgcccg cgggttctcc tgcagtctgg ctgagattca ggatccggat
     6721 gcgtgcgccc atagagatca ggaaatcaag caatgcggat cgggtcgggt tcagacccac
     6781 tccctcgatc accagcttcg agccagggac caacacggcc gccacgagga agaaggccgc
     6841 agaggaaagg tcgctcggaa cagtgagttc gcgcccgtta agtttcggac gtcccttgac
     6901 ggtaatggtc tggctgagta cctcgacgtc ggcgccgaac tcccgcaggg cgacctcggt
     6961 gtgatcacgg gtctggacgg attcacgaac aatggtggtg ccgtcagcaa acagtccggc
     7021 gagcagaacg caactcttga cctgcgcgct cgggacgggg agcttgtagt cgatcggccg
     7081 caaccggccc ccgcgaatct cgaggggcgg gaatcttccc tcgcgggccc ggatcgaggc
     7141 gcccatctcc gcaaggggct tcatcacacg atccatcggc cgccgggcta aggattcatc
     7201 gccggtgatg tgcgtggcga aggactgtcc ggcgaggatg ccagacaaca tgcggatggt
     7261 cgacccggaa ttgccggcgt cgagaatgcc ggcgggttct ttcagaccgt ccagcccgcg
     7321 gccgccaatc gtgacctggc cgccgtcgcg ctccaccgga accccaagcg cccgaatgca
     7381 cgccagggtc gacccacagt cggcgccggt cgaatagttc cgaatgctcg tcacaccttc
     7441 ggcgatagct cccagcatcg cgtagcggtg tgagacggac ttgtcaccgg gcagccgtat
     7501 cgaaccggac agcacccggg ctggggagat cctttcttcc atggaagact ccccattgta
     7561 cccgcgtgcg gaaccgtcgt cagcctatcc cacagtctta tgccgtcagc gcaagaacgg
     7621 gcgcgaagtc agggaaggcc cgtggttcgc gatagcgttg aaaccggtac cctaccccga
     7681 aaatggtctc gatgtagtgg tcgccgtggt ctcccagctt tttccgcagc cggcgaatgt
     7741 ggacatcaag agtgcgagtc cggatctcgc ggctgtaacc ccagatctgc atcaacagga
     7801 cgtcgcgggg cacaatctcc cccgtgttat ccactagtag cgcaaggagt tcatattcct
     7861 tgcgagtcaa taccatctcc agggagtcca ggtacaccct gcccctctcg aaatcgacct
     7921 gcagatgctc gtcgcgatac gtcttcggat tctcgatttc ttggtaactc ataccagctc
     7981 cgtagcctct ctaagatcga tgctacgggg tcggagtcgg aaggtggtcg aaaacgtgta
     8041 aaaaagatgt aaaacccacg ctactcgatg acaaaagggc ctgcattctt caccttcacg
     8101 tctccgactc ttgcggcatg ggctctcact gacacggtta caaagcgcgg ggcatccttc
     8161 gtctggaact gaatcgccga ttcctggtcc gggtcgagga tctcctcgtc gatcggacct
     8221 ttcttggtgg tcaccacgcg gttgtccgga tcgcgaaaat cgaactccac cacaagtctt
     8281 tctgcaggca tttccccgat gttcttcacc cggccgtcca gggtcacaag ttcctccgtt
     8341 cggtggacgt tcaactcgag gatctcgatc tcaggcggcc gcggcttgcg cttttgttgc
     8401 acggcgaaca acaatgtggc gagcacgatc agaactgtga gggcgcgctt catcaggtca
     8461 tagtctacca ggaggtattc ggatgataac ccgccggaat tggatgctgg gcgcggcggc
     8521 cgtgtcctcg gccactgcgg tccagtcgaa gccgaagcac atcgcggtcg ccagttcgaa
     8581 cggcctgcga gcgtgcgctc gcgcggtcga actcctccgc cgcggggccg acacgctcga
     8641 tgcggccgtc gccggagtca acatcaacga agaagacccg gaggacacct cggtcggata
     8701 cggcgggctc ccaaacgagg aaggcgtcgt cgagctggac gccagcgtaa tgcacggtcc
     8761 tacacgccgg tgcggctctg tggcggcact gcgcggcgtc aagacgccgt caaaagtcgc
     8821 gaaactcgtg atggagcaga ccgaccatat catgctgacc ggcgacggcg ccctgcgatt
     8881 tgcgaaggcg ttcggcttcc gcgaagaaaa ccttttgaca gagaaagcac ggatcgcctg
     8941 gctcgtctgg aagcaatccc ttcgggacgc ggacgggcac aacaactgga cagacggact
     9001 ggacgcaccc gcgtcccggc cctccgttaa gccgcggtcg agcctccgga tgctgttccc
     9061 gggtgtggac gagacgacgc tcgcctgggc cagggacgtc gtcatgcatc cgccgacggg
     9121 taccatcaac tgcctgacgc tgaatgagaa gggcgagatg gccggcgtga cgacgaccag
     9181 cggccttgcc tggaagatcc cgggcagggt gggcgattcg ccgatcatcg gggccggcgt
     9241 gtatgtcgac ccggacgtgg gcgccgcggg ttccactggc cgaggggagg agaacatccg
     9301 tgtcgtcggc gctcatacga tcgtcgagaa catgcgccac ggaatgacgc ctaaagaagc
     9361 ctgcctggat gcgctgcgtc gcgtcgcgcg caactatgac aacgacgccg ccctgctggc
     9421 gaaattcgat ctccggttct acgcactccg caaagacggg cagtacgcgg gagcggcgct
     9481 ctggaacggc cgcatatcgg gcgggaagtt tgctcacgcc gaattctcag tcaacagcgg
     9541 aggagcgagc cgtctcgaga agtgcgcata cctgctggag cggaagtaac cgcttcggcg
     9601 ctcagcgggc cagaatgtcc cgcagagtga ttgctccgag ggcgagggtc agcccgacaa
     9661 gaacgaccac cgctgtccag gccaccacgt tataggttct tgggttgacc cactccccca
     9721 tcagaccctt ccggttcacg agcaagacca tgaagatcag cacgaagggc agcagaacgc
     9781 cattgatcac ctgggagaac aggatcatcc gcaccagcgg aagctgcggc agcagcacta
     9841 ctccggcacc gaccgccacc agcagcgtgt acagccagta gaagaccggt gcctcgcgga
     9901 aacgctggtt caggccggat tcgaatccga gcccttcaca gacggtgtac gccgtcgaga
     9961 gcgggagaat gcaggcggcg aacagagaag cgttgaacag cccggcgcag aaaagaaggt
    10021 aggcgtaatc tccgaacggt ttcagcccga gcgcggcgtc ggcagcgtcc ttgatgtccc
    10081 tgggtgacac cgtccagatg gagccggcgc aagcgacgat gatgaaaaac gcgatcaccg
    10141 ccatcgcgat gcagccgacg attacctcaa tcctcgattc cacgtattcc ttcgccgtca
    10201 ctcccttctc aaccaccgcg gcttgcaggt agaactgcat ccagggagcg atcgaggttc
    10261 ccactagccc gataacgagg gtgatgtacg ccgtgtcgaa catcagcacg ggacggacgc
    10321 tgtagatggc cgcctccgcc cagttcggct tcaccagaat gccggcgatg acgtagctga
    10381 cgtacagcgc ggaagcaaac aggaacacct tctcgacgct gcggtacgtt cctttgacga
    10441 caagcagcca gacggcaacc gcgccgaggg gcaccgagat atagcggctc acgtggaaca
    10501 actcgagcga gctggcgatg ccggcgaaat tcgccatgat gttggtcagg ttcgtcagca
    10561 ggagggccgc catcatcaga aacgtcgctc gcagcccgaa ctcttcccgg ataaggtccg
    10621 acaggccctt gccggtgact gcgcccatcc gggcgcacat ctcctgcgtc acgatgagca
    10681 ggatggtaat cggtagcagc gtccagagcg ggagatagcc gaacttagca ccggcctgcg
    10741 aatacgtgta aattccgccg gcgtcgttgt cgaccatcgc ggtgatgaag cccggtccga
    10801 tcacggagaa gaacaccgcg atgtggtagc gaaaccgttt cagcatgcca tccgtcgctc
    10861 atttctgtcg caagaccgtg atgatatcgt ccgccgtgat cacgccggcc agttttcctt
    10921 cctcgccgat caccggcagc gccagcaggt tgtacttgtc gaatagctcc gtcacgcgat
    10981 cctgcttctc gtcgaccgag acttgaatcg gtgtgtcggt agccagttcc ttgagcctgg
    11041 cgggtccgga cgcgatgaag agccgcgcca gcggaacaac cccgacaagc cgttcttcgc
    11101 cgtctatcag gagcagtgtg ttgagagtct cgagcagctc ctcgttgcct ttcagggctt
    11161 ccagcgcgtc ggagaccgtc gcgttttcgt gcagcgcgac atactcggtg ttcatcagac
    11221 cgccggcggt gtcttcctcg aactcgagta gctcgcgaac ctccgttttt tcctctggct
    11281 ccatctcttc gaggatctcc tcggaggttt cctgttccag ttcggcgagg acatcggccg
    11341 cctggtctgg agacatttct tcgacgatat cggctgcctt ttccgtgtcc agcgattcga
    11401 ggatgcttgc ctgcatcttt ggatcgactt ccgacagcgc ctcggcagcc acttcgctgt
    11461 cgatggcggt gaagatggct tcccggtcct ccgggctgag ttcttcgacg atgtcggcga
    11521 ggtctgccgg gtgcatctct tcgagcatcc ggtgcgtaat gttcaggcgc aggcgccgct
    11581 gcggatccgc ctccacaatg ttgcagaact cccagcggat cgaattgggc gggatcggcc
    11641 gctgaagctt ccgtacgacc cgccgcggca ggagcccctg cacgatcctc cggaagatgc
    11701 ttcgcatgcc gacgtcaacc tcggaaacga agagaacgtc gcgatcttcg tccttccgga
    11761 tctcgaacgt gacgtcagtg acgcggacca ctttgcgccc agagacgtcg atgatctgct
    11821 ggtcgagcag gtcccggacc agccgcagca tgtattcgtc ggaatggtag ggggtgagca
    11881 gttcgtcgcg aaggtagatg ccgtcgaggg agatggatcg cacctgatcg tgccggacag
    11941 tcagccaggc ccacccgccg ccgaccaaaa aacggtccac acgggcggaa tcgatgagtg
    12001 gaaccagagc cgcgtctttc acgcggccga tgcggtggcg cttcaggtca taaaccttca
    12061 gcccgaccag ttccgtaagg tagagcaggt tctccgccat cgaaaacgtc cagcccgccg
    12121 gggcgttccg agcgctccac gggcgtctcc cgtactggcg gcctgctgtg gtgcgctcta
    12181 catctcttct tctctactac tatgaactat cccgccggaa acccgcaata cattattgga
    12241 gtttctgctt gagcaattcg ttcacggtgc cggggttggc ctgaccgcgc gtcgccttca
    12301 tcacctggcc gacgaagaac ccgagcacgg ttgatttccc gcctctgtat tgctccacct
    12361 gcttcggatt cgccgccagc acgtcatcga ggatcttctc gagggcggtt gtgtcgctga
    12421 tctgccgtaa accttcgcgg tcgatgatca cgcgcgcgga ctcgccgctc ccgagcatct
    12481 tggcgaagat ctccttggca agtttgccgg aaatttcacc cttcgcgatg agggaaatca
    12541 gctcgccaag atgctctgca ctcaccgaag actcggcgat ctccttgccc tgttccttta
    12601 gcgcgcccgc aaggtctccc atcacccaat ttgcggccac acgcgggtcg ccggaagcct
    12661 tagccgcacg ctcgaagtaa tcgctgagtt cgtgagtcgc cgtcaggact tgggcatcgt
    12721 actcgcgcag cccgtacgac tcaatgaacc gcgccctccg gctcgccggc agctccggca
    12781 tttcggcctc gatccgggcc cgccattcgt cgctgacgcg aagcgggacc aggtcgggtt
    12841 ccggaaaata ccgatagtcg tgggcatgct ccttgcttcg catccctgca gtttctcccg
    12901 tatccgggtt gtagaggcgg gtctcctgga tgattctgcc accgccctcg atcaccccca
    12961 cctgccgggc gatctcgtag tcgagcgcca ttttgaggaa gcggaacgag ttcaggttct
    13021 tgacctccgc cttggtcccc aacttctcgg cgcccttcaa ccggactgac acgttggcgt
    13081 cgcagcgcag gtgtcccttt tccatgtcgc agctcgacac gtcgacatac tgcatcacct
    13141 gcttcagttc agtgaggaac gcatacgcct cgtccgagct gcgcatgtcg ggctcggtga
    13201 cgatttcgat cagcggcgtt ccgcaacggt tcaagtcgac ataagagtag cggtcggagt
    13261 cgcggaaacc ctcgtgaacg cttttgccgg catcgtcttc gatgtgcacg cgcgtcacac
    13321 cgattcgctt ccggacaccc tccacttcga tctccaccca gccgtgctcg gccaggggct
    13381 cgtcgtactg cgagatctgg tagcccttag gcaggtcggg gtagaagtag ttcttgcgag
    13441 ccagccgtga gacctttcga atctggcatt gcagcgccaa gccggccttc accgccatct
    13501 cgactgcctg ccggctcagc actggcagcg ccccaggaag gccaaggcaa accggacaga
    13561 cattcgtgtt cggtggcgcg ccgaagctgg ttgggcagcc gcagaagatc ttggtcctcg
    13621 tcgccagttg gacgtggact tcgagaccga tgaccggctc gtacttcgcc agcacctccg
    13681 gcgccacgag ggccgcggtt ggggacgaca ttccttgatt atagggtgct gcgtgtttcg
    13741 cagcctggcg agacgcagta gaatcgaagc gccatgctca cctccagccg acgacaattc
    13801 gtctccgggg ccgccgcgcc gttcctgagc ccgtccgggc gcggagaacg ccggcccaac
    13861 gtcgtgatgt tcatgaccga cgatcacggc gcctgggcgt tgggctccta cggctgcccg
    13921 gaaatggaca cacccaacat cgacaggctg gccgccggcg gcgcccgttt cacccgcgcc
    13981 tatgcctgca ctccagtctg ctcgccgagc cgcatgacct ggctgacggg ccggattccc
    14041 tcggtccacg gtgtgcagga ctggctcatt ccctccgaca gctttggggc acgcacgcgc
    14101 cggtggctcg aaggacaccc tgcgtactcg gaagtcctag ccaagaatgg ctacagcctc
    14161 ggtatgtgcg gcaagtggca catgggtgac gacgagcgtc cacaggccgg cttcacgtac
    14221 tgggcgaccg tcccgggcgg cggcggacct tacaaggacc ccgagttcgt caagaaaggc
    14281 cggcgggccg tgattcccgg ctacaagacg gatatcgtcg gcgattgcgc tctcgagttt
    14341 ctggaacagg tccgcggcaa cccgttcttc cttctcgtgc cgttctacgc tccccacacg
    14401 ccgttcaact accaaccgga ggaataccga cagccgtacc tcaagtcgag gttctactgc
    14461 ttcccggatc cgcccgccca tccgtggcag aacccggggc tcaagtcgca ccatgcgaac
    14521 cgagcgagca agctcgcata ctccgcgctg attaccggcg ctgaccgcaa cgtcggccgg
    14581 atagttcgaa aactcgaaga gatgggcgtc cgcgaagaca cgctcgtcat cttcactgcc
    14641 gatcaaggat ggaatgccgg ccaccatggg gtttggggaa agggcaacgg cacagtgccg
    14701 tttaacatgt atgaggaatc catccgcgtg ccgttgatct ggaatcaccc tcggcgcgtc
    14761 cgttccgggc aggtgctgcc gccgatggtc tcctcgtacg atttcttccc gacgatcctc
    14821 gactacctcg gcctgtccgc ctcgcccgat ccgaagcgcg tcggacgcag ttacgcggca
    14881 tttctgcgtg gcggcaagcc ccgatggcag aaccgactgt atttcgaatt tgcctacgtc
    14941 cgcgccgtcc gtaccgaaaa cctcaagtac gttgagcgga caaaagaatg gccagccgag
    15001 ttgtacgacc tcgaggccga tcccggcgag acccgcaacc tgatcgacga ccccggctat
    15061 cgtaagcagc ggatggcgct gcacacggag ctctccggat ggttcgaccg catgggtgcg
    15121 ccaccgatcg aggcatggcg ctccacgacg aagcaggaac ttaccgtcta cagccgctag
    15181 aataggggtt tatggccaaa gaatatgatc tgaccccgag gcgcgtcccg agggtggaaa
    15241 cacgctaccg acgcattgtg actgagattc cggctcccaa gtcggtgcag atccttgaac
    15301 gcctgcaccg ctacgaaccc ctggcgatgc gcggccagcc gccgatcgtg tgggaccatg
    15361 ccgagggctt tcaggtgtgg gacgcctggg gcaactgctg gatcgactgg tcgtcaggtg
    15421 tgctgatcac caacgccggc cacggccgca aggaaattgt ggaggccgtc gccggccagg
    15481 ctaacagaaa gctcctgacg acgtactgct tcccgacgga aacgcgcgcg caactggtcg
    15541 agcggctggc ctcgcttctt cccgagccgc tcaagaaggt ctttctgctc acaaccggtt
    15601 cagaggcggt tgagtgcgcc atcaaactct gccggacaca cggcgtgaaa ctgggaggcc
    15661 ggtccaaaaa cgtgatcgtc agctatgaca aggcgttcca cggccgtacg ctcggttccc
    15721 agcaggctgg tggcattccg gcgctgaaag agtggatcgt aaatctggac ccgggctttg
    15781 tccagatgcc gttcccagac ggcttccgga cggaagacac ctcattcgag tacttcgagc
    15841 ggagtcttgc ggcggcgggc gtaaaaccgg aggacatcgc gggcatcata ctcgagacct
    15901 accagggggg cagtgcctcg ttcgcgccgc cggcatacat gcaggccctg cggaaatggt
    15961 gtgaccggca caaggtgctg ctggtatgcg acgaggtgca ggccggcttc ggacgaacag
    16021 gcaccatgtg gggcttcgaa cactacggga tcgtgcccga cctggcgtgt ttcggcaagg
    16081 ggatatcgag ttcgctgccg ctgtcagccg tagccggcag gcctgacgtg atggacctcc
    16141 acccggccgg cagtatgacc tccacgcaca cgggcaatcc cgtgtgctgt gctgcggcgc
    16201 tcgcctcgat cgaattggtg gtcaaggaga atctcgtcga aaacgcgcgg aagatgggtg
    16261 atctgctcca ccgaaagttg aacgaactga agccgcggtt cccgcagatt ggctgggtgg
    16321 acggcaaggg cctcgtggcc ggcgttgcct gtgtcaagcc gggcacgaag gatccggacg
    16381 gcgacctggc atgggaggtc gtccggcgtt ccatcgagaa gggcgtgctg atgttctcgc
    16441 cggtcggctt cggtggcgga acggtgaaaa ttgcaccgcc gctcgtgatc aacgaagatg
    16501 ccgtgctaga gagcaccggc gtactcgaag aggccttcgc cgaggcgaca gtcgcgttgg
    16561 aggctgccgt ccgatgagca aacccgatgt cgtcgtcatc ggcggcggga tgattacgaa
    16621 cgatcagatc ctgccgtcgc tgtaccagat gcagcggtcg gggcacatcg gcgaaatcaa
    16681 gatctgcgcc ctgaatgctc gcccgcttaa ggacctggcc gattcagcga cgttcaaacg
    16741 cgcctttcca ggccagtcct tccgatcttt cccggacatc gcgggtgacc tcgacaagcc
    16801 ctacccggat ctcttccggg aagtaatcgc gtcgctgccg ccgcggaacg tggtggtcgt
    16861 cgcggtgccc gatcagcttc acttcaacgt catcatgacg gcgctgaagc acaatcagca
    16921 cgtatgtacg gtgaaacctc tggtgctgaa ggctgccgag gcgctcgaga tcgaacggga
    16981 agcgtacagc cgcgggctcg tggttggcgt cgaataccac aagcgcttcg acgaccgcag
    17041 cctgctggcc cggcggcggt accgcgaggg ccggttcggc gagtttcgcc tcggcaccgc
    17101 ctgcctgctt gagaagtggt attaccgcca ttccaatttc cagaactggt gcacggtcga
    17161 gaactccgac gccttcacgt acatcggatg ccattacgtg gacctggtgc acttcatcac
    17221 cggcctgcta ccggtcgcgg tgagcctata cggcatccgg gataagtatc caaacggcaa
    17281 agagggattc ctctggaccg acgcgcgtgt cctctggaac aacggcgcgt gcctcaacgt
    17341 gcagaacgcg ctcggcttcc cggatgaagc gcccggcacg aatactcagg gtatgacgat
    17401 gtattgcgct ggggagacga acggcacgtt gatttcgcat tccgaccaat tccgcggctt
    17461 gaagtactgc tacgtcaacc gggacgactc gccgggcgcg acggtctacg ctgaaccgag
    17521 taccgactac ttccaatacc tcgatctcgg cggtcccggg atgatccccg tcggctacgg
    17581 ctaccgctcg gtggagtaca tcgtcaaagc ggccatccgc gccgagtcgg agacagccgg
    17641 cttggccgaa gccgattcct tggcaaagcg gcaagccatt ctcaaggagt atgacgccgc
    17701 gggcgtcatg gccactccgg ccaacagcag ctataacgaa ctggtgatcg aagcggggcg
    17761 cctctcgatc ctcaacggcg gtcgagaggc cgtcatcgag tacggcaaga agcccggcgt
    17821 ccgccttcgg aactgagatg cacaaccggc gcctgcagct ctcgcgggag gagatgcggg
    17881 cgctcggtta ccggatcgtc gatcgactgg tcgagcacct cgacaaccaa accagccagc
    17941 gggtggctac gaaaggcgtg cgcgccgaac tcgagagccg cctgcgcgag tccctgccgg
    18001 aagaacctgc ctccccggac gacgtcctga agcagctcga agaggacgtg ttcagccaca
    18061 tgctgcacgt tgaccatccg cgcttcttcg ccttcgtccc cagtcccggt aactacgtgg
    18121 gcgccttggc cgacgctctt gcctccggga tgaacccgtt cgtcggcaca tggctcggcg
    18181 cttcgggacc ggcgcaagtg gagttggtca cgatcgactg gctccggcag atctgcggcc
    18241 tgccggatgg atcgggcggc atcttcgtga ccggcggctc ggtagcgaac ctgatcgcgc
    18301 tggcggctgc ccgccacgca acgctgggcg aagacacggc acacgccatt gcctacttct
    18361 ccgaccagac tcattttgcg gtcgagcgcg gcctccgagt gctcggcttc gcgcctgcgc
    18421 agctccggag gctgccctcg gacgatcgat tccggctcgt gccggccgaa ctggaacgag
    18481 cgattcgaga agatcgcttg gctggccgca ggccgttttg tgtaatcgcg aatgccggaa
    18541 ccaccaacac gggcgcggtc gacccgctgc cggtgcttcg ggagatctgc agccgcgaga
    18601 gtctgtggct tcatgccgac ggcgcgtacg gagcggcggg gatgctctcc aggaaaggcc
    18661 gcgctctgct cgacggattg ccacaggtcg attcgctggc gctcgatccg cacaagtggc
    18721 tatttcaacc gttcgagatc ggatgtgtgc tcgtgcggga cgccggtctg cttcgcagca
    18781 cgtttcaggt gcggcccgaa tatctcaagg acgctctgcc caccgaagac gaggtcaatc
    18841 tgagcgatta tggcgtgcaa ttgacgcgaa gcttccgcgc actgaagctc tggatgtcac
    18901 tcaaggtttt tgggctttca gcgttccgtg aggcagtcga ctggggcatc aggctcgccg
    18961 agatcgccga gggaaggctg cgaagctcgg ggaaatggga gattgtctcg cccgcccagc
    19021 tcgcgatcgt tgcgttccgt tacgccggcg gtgacgacgc cgtcaacctc cggatcattg
    19081 atcgaatgta cgacgacggt tttgccttcc tcagctcaac gattctgtgc gagcgccccg
    19141 ctctgcggct gtgtacgatc aacccgagga ccaccgaggc ggatatcctc gaaacgatcg
    19201 accggcttga gcggttcgca cagtagttgc ctgattcttc ccgtcaaaga tcctggaaac
    19261 ctgacgcggc gcgatcacgg aagttctgcc tagcgctagt ccgtactagg acaccagagc
    19321 gacaccacgc gcggaatcgc acgacattct ccaaataatg atatgacgac aaggtgaaat
    19381 aaggtcttcc gcttagctca taagtaccat tctatgtatc aactagactt accttagcgc
    19441 ctaagtaccg aggtggtgat agtacgatat ctaagtgcaa acaggggtga ataccttgtt
    19501 ttcggagtgg gatctcaggc acactggagg cgattcaagt tcggttactg aggtgaacga
    19561 tgcgtcggtt gctcctagtc ctgctcgtcg cggccttgcc gtcaccgggg atgtctccaa
    19621 agacaaaacg ccgtctgacg tttgccgccg tggcagccgc acagatgctg gacctgcaca
    19681 cctcgctggg aaggaaggag ggcaatcctc tactgcgagg cccgaatggc cggttcgctg
    19741 ccggacgcgg agtgggggtc aaactcggaa tcctttccgg gctgctggcc gcgcaggaac
    19801 taaggccagc cccttactgg aactgggtga atgttacgta cgcgggcgca agcgccacga
    19861 tcgcggtccg gaattatcgc gtgagcaagc cacctgagta cctggggccg gctcggtgag
    19921 ctggcgggcc gaagtcggat tactgaacca ccccaccgcc gccaccttgc ggctcaaggc
    19981 ggatctcgcc gaacgccccc tcgtactgct ggatgttctg ttggaggagg ttggcgagcg
    20041 ctttcgcctg ctgcggactt aggtagaccc cctggaagtt gcggatattg acgctatcgg
    20101 gagcggtctg gttgatggtg ccgaacagca gcaggaagtc ccacaggttg gcgcggacct
    20161 ggacgctgtt cgcgtatccc tcgcggtagt cctgcgagaa cgataactgg atcttcggct
    20221 gggcggccgg cgtcatgagc tattttcctc cacctttggc gcggtcaatg tacttcaggt
    20281 tctccacgtc gagccggatg aggtctccga ccttgatgaa ctgcggcacc atgatctcga
    20341 cgccattctc cagcacggag ggcttccatg tactgtcttg ctgtgcatgc accggagggg
    20401 cagtgtcggc aattcgcacc tcaatgatgt cggggaacaa gacgctgacc ggctgtcctt
    20461 cgatgaactc cacgggcacg agcatctccg gcctgagaaa tctggcctga ggtccgatga
    20521 tggccaccgg gattggcacc tgctcgaagg tttcggggtt catgaaatag caggcgtcgg
    20581 cgtcgttata cagatactcc atcgactgtt tctcgaccgg cagatcctca agcttcaggt
    20641 ccgaccggaa actgtgctcc catagggtgc cggtcgacag gtttcggagg cgggcatggg
    20701 tggctccccc cattttgccc tgccctggat ggtagtcggc gatgagcacc ttgtatgcct
    20761 gtccctcgta ccgtactgcc ataccggcgc ggagttgcga tgctgtgacc atgcattcct
    20821 ccggacaaga gcatactacc gcgactcggc tgcctaagaa agccccagtc agtcaccggg
    20881 ccggggccaa aagccactac attgcttcgc cacaagacca gatctacaca ggaaatagca
    20941 ggaaaagctc agagagaatg gcggggacga cggggctcga acccgcgacc tccgacgtga
    21001 caggccggcg ttctaaccaa ctgaactacg tccccgctag gttgtcggat cgtctttatc
    21061 ctaccacgcc gtacaaccgt tgtcagttgc ctgatggtat aaaatcggga cgatgaaacc
    21121 cgcctgtctg attcccgcca tgttcgccat cctgggtgtc agggcgtccg gcgaggtggt
    21181 gcgcgtcgat cccgctctgg accagatcgt atcggaagca gcgcaaatcg agaaagtcgc
    21241 tggaggtttc gccttcaccg aggggccgat ttgggctcgc gatggctatc tcctcttcag
    21301 cgacatccca aacaatgtga ttcagaagtg gactccggaa ggtaaggttt ccctgttccg
    21361 aaagccgagc ggctacgaca aaaccgatgc gccgaaaggg gcttttatcg gctcaaacgg
    21421 cctcacgctc gatcgcgagg gtcggctcac catttgcgag cacggaaacg gccgcgtcac
    21481 gcgaatcgaa aaggacggca aactcaccgt cctcgccgcg aagtacgagg gcaaacgact
    21541 caacagcccg aatgacgcgg tgtacaagtc gaatggatct ctgtacttca ccgatccacc
    21601 atacggcctt ccaaaggagg accaggatcc gaaaaaagag ctgccattca acggcattta
    21661 ccggatcgcc gacggcgtgc tccagttgct gtcgaaagag ttgacacgcc cgaacggcct
    21721 cgctttttct ccggacgaga aatatctcta tgtcgccaac tccgacccgg cgcgaaagat
    21781 ctggatgcga ttcgaagtga agctggacgg aaccatctcc aagggccgcg ttttctatga
    21841 tgtgactcgt cgcaccgagg aagggctgcc cgacggcatg aaggtggaca ggcggggcaa
    21901 cctttactgc accggaccgg gtggcgtctg gattttctct cccacagcaa aacaccttgg
    21961 cacaatccgg ctccccgaga cgccggccaa ctgcgcatgg ggcgatgccg acggcaagac
    22021 gctttacact accgcccgca cgagtctcta tcgtatccgg ctgaacgtcg aaggcgtccg
    22081 gccgtaaatt ctacgaggcg gtattatccc cgaaaaggcg tgactatggg tacgtatcgc
    22141 ggatggccgt gtcttcttac gcttgtcttt acagccgctt cggctggtgc ggactctccg
    22201 tgggtcgaga cccgcacctt ctacacgaat gcggaaggcc tgcccgacac ggaagtgctg
    22261 gcggtcgccg ccacatccgg cggcgacgtc tatgtcggtg cccccaaagg tttggctcgt
    22321 ctctcacgtg ggcagtgggt caccgttccg gcccttgccg gcactcgtgt cgaattactc
    22381 gtaccggacg ggccggacct gctggttgcc acgagcgacg ggcttcaccg tttgagcgcg
    22441 aaggctgcga cgcggttcgt gaagccctga gacgcgatca gccggaggtg gaggccgaaa
    22501 cggaggatgg ttgcccgggc gcaggggcag cagccgtctt cgcggtctgg ggctgtgtga
    22561 tgtcgatgct gcctttgaag aaggcgccgt cctcgatgac cacgctcgcc atcttgagat
    22621 cgccggtcag tcgcgagttc ttccgaagaa tgatgcggtc gctggcctcg acgttgccgt
    22681 gcacggcacc gtagatctcg acctcacggg cgcgaatcga ggcgcgaatc ttcgcgtttg
    22741 gaccgatcgt gagacggttc tgaccgacgt caagtttccc ttccatctcg ccgtcaatcg
    22801 aaaggtcctc gcgactcagg atctcccctt tgaccaccat tgattggccg atggccgcgg
    22861 gacgccgctg agtgtccgcg gcggaacgag ccggcggtgg cgggggcgcc tgcattacct
    22921 ccgcgggcga tgccgggcgg cgctgctcgg cgcggggatg gtcgtcatcg gatgcgcgtt
    22981 tgttccacat gctcatgatt ccgtcctcgt gaagagttaa acatattatc gcagcacgcg
    23041 aacggccaaa cgagaacgcc gctaagccga tgagaatatg cggagaggcc agcccgttgc
    23101 agccgaagcc cgcggaagtt tcttccccca ctgggaagaa aaggccggcc gctggggggt
    23161 tctttgatac cgccgacgct cccatcggcg agcggttcct ctttaacggc gtgcttgccg
    23221 tgctggtctt gcaggccacg tgttcttgca agggcaaccg agcgttactg tcgcagcgtt
    23281 gaccccgcgc tcggaagacg agagaataga atcttttgtt ccagtgtccg agcgaaggtg
    23341 tcttcggccc gagcgaaata tgagactaat acccagggac gagaagttct ttcagctctt
    23401 tttgaagcag gtacgcctga tttcgcaggc gtcggatgtg cttctgacag gtgtgaaggc
    23461 aggcaatgcg aaaatggcga cggcctgcga agtcatccag cggctggagc acgacggtga
    23521 cgacgtaatc cacgacattc ttacccgcct gaaccagacc ttcattactc ctctcgatcc
    23581 ggaagacatt cactcgctct catcgagtct ggacgacgtt ctcgacggta tcgaagatgc
    23641 tgcctatcag ctcacggctc acaacgtcga gccgattccg ccggcagttg tcgaactctg
    23701 tgagattgtg aactattgtt ctcattcgat cgccctggcc ttccaggcac ttcacgacgg
    23761 aaaagcgctt ctggaacatt gcgtggagat caaccgtctg gaaggcgtgg cggatctgct
    23821 ggttcgccgt gtgttagccg agctctaccg aagcgacatt catccgatgg ctttcattaa
    23881 gctgaaagag gtctacgaag ttctggaggc cacaactgac cgatgcgaag atgtggccga
    23941 tgcgctgcag ggcgttgtcg tgaagaatag ttaggctgcc gccatcgcca tgttgtcact
    24001 ggttgtcatc atcgttgcgg cggcgctgat tttcgactac atcaacggct ttcacgacgc
    24061 agccaattcc gtcgcgactg tcgttgctac tcgcgtgctc tccccatttc aagccgtact
    24121 gtgggccggc ttcttcaact tcgcgtcagt cttcagcttt ggcaccggtg tggcgcacac
    24181 cgtgggcgca gggatggttg acatgaccat ggtgacgccc tacgtaatcc tcagtggcct
    24241 tattggcgcc atcatttggg acctgttcac ctggtggctc ggcttgccca caagttcctc
    24301 gcacgccttg ttcggaggtt atgcgggtgc ggccatggcg cacgttgccc acctcaaagg
    24361 ggtcgcccat gccgccgatg tcatcctgtg gaagggctgg ataggcaccc tttttttcat
    24421 ggtcgcctct cccatcattg gattggtgct cgcgtacctg ctgatggtct gtatttattg
    24481 gctttttcgg agatcaacgc cgcgaaagat ggatgtctac ttccggaagc tccaactttt
    24541 ttcagcggcg gtttacagct acgcccatgg cacgaacgac gctcagaaaa cgatgggcat
    24601 catcaccctc gtactcgtga ccggcggata tctcgagaag ttcgaagtgc cggtctgggt
    24661 catgctcagc gcctgcgggg ccatcgcgct gggtacactg acgggtggct ggcgcatcgt
    24721 acgcacgatg ggagtcaggc tgactcgctt gcgcccgcgc ggtggcttct gcgctgaagc
    24781 agcggcagcc acgtccatct tgctagccac gcatcttcac atcggtgtgt cgaccacaca
    24841 tgtgattacg ggcgcgatcg cgggtgttgg ttctatccgg cgggtaaaag ccgtgcgatg
    24901 gggcgttgcg gccaacatca tctgggcgtg gatctttacc atccccgcag ccgcgctcgt
    24961 ggcggcgtgc tgcttcgcgc tcatccgcgt gttcgtccgg gaaagctgag aatctgtttc
    25021 gccggccgcg agcgcaaccc cttcagccgg cggcattctc gttagccccg ctattctgtt
    25081 ggtgcccggg gtgggaattg aacccacacg gtggttgccc accaaaggat tttaagtcct
    25141 ttgcgtctgc cgatttcgcc acccgggcag agactgtcac gctgctgtgc cccatcagcc
    25201 tcattatagt gaacacatca gccccggaat ctccgaagtc gggttccaaa ggtgtggcgg
    25261 aaacagcgcg gcacgaccgc tgtgagtgac gatacctggg tcgccggatc gaacccgtag
    25321 aatttgctcc gcagcgtaaa aaatgcgggt gtttccagtc aagcccgtgc acgaagctgc
    25381 ggtggcgatg aacggaaaga attggagcat caatacttgg gactgatgga acgtcacatg
    25441 ctctcgctga gtctgcgccg cgattcggcg actcaacgtt agaggggaga aggacatgtc
    25501 aagaattgtg tccctactgg tgttttctac agtgttctcg atgttggcgc tcgcggagag
    25561 ctggtcggga aagctgctgg acgccaactg ctacgaccaa cagaaacaag ctagtgcgtg
    25621 cagtgccacg agtgaaacga cggcctttgc gctggatgcc gccggcaaga tctacaaact
    25681 ggatgccgcc ggcaactcaa aagcctctga tgctctgaag aatcgtgctg atcgggcgac
    25741 cgacccagcc aagccacagt cgaaagaggt gatggcccaa gtcactggtg ctgaaaaaga
    25801 cggaaccatt gcggttgaga gtattgaagt acggtaacct agcacctggg agccgtcctc
    25861 gaacggctcc cgatcttctc ctgcattgag ggctcgggcc gagaggagga ttgagcggac
    25921 ggtagcgagt gaactgtgcg gaagacaccc ttcgtttgcc cgaggggtgt gatacgctca
    25981 cgtagatggg gtcctgtgtt ctgcgtcagt tattctggcg cttttcattc ggtctacttg
    26041 tgcttggaag ctcagttggc aactgcgtgg ccccggtgac gccgcgcgag ccggatgtgc
    26101 gagagcgtga gcgactggca gagactttcc tcagagaaaa gcttgcgctt tggcagcggc
    26161 gtctacacct ccaggattgg accatttctg ttgtcacgtc taactcggac gccctgagac
    26221 ccgggacgcg gggcaatatc cgatgggacg cagatacgaa gactgctgcg attcgggtcc
    26281 tggacgcctc cgagtaccgc atgcccttcc gcgccgcgct gaaggacatg gagttcacta
    26341 tcgttcatga attgatccac ctgacactct cacctctgcc gcgtagcgaa agcagccgca
    26401 gcaaggaaga gtacgccatt aatcggatcg ccgacgcgtt gctgcaaggt gaccgtgacg
    26461 actgacctag gcggctccgg cacaggccca aaggatttta agtcctttgt gtctgccgat
    26521 ttcgtcaacc gggcctactg acattcaaac acttgtcgga cgcgcggcgg gcgggccgat
    26581 cacgatataa ttcagcatca ggccgtagca ggcgccctag tagatagagg agtgcaatct
    26641 ttggacgcgt ctcccgcacc tcattttgaa gcgttgcttc aacgcgatat cgacgtcatc
    26701 cgtaacaggc tgctcgaaat gttgagactg gatgaggagg cgttgaagcg ggcctttgac
    26761 gccgtcctgt cccgcgatcg ccagctcgcc tattcggtga tcctgcgtga ccaggaagtg
    26821 gactcccggg aaaccgaact ggaccggctc tgtatcgagt tcatcatgag gcaccagcca
    26881 gtcgcaagcc acctgcgctt cgtttacggg gccagcaagg tgatcagcca actcgaacgc
    26941 gtcggcgact acgcagaaag catcgcccgg caggcgttgc ttctgagctc agtttcctat
    27001 gaggtacccc gggaaatgta cagcgagatc gcgaaactgg cggtctcgat gctgcacaac
    27061 gccgttcgcg cgttcgtgga caagaatccc gacctcgccc gagccactat gctcagcgag
    27121 ccgcgggtga atcaggtacg tgaccgcatc atcgtcgaag tgctccagtg gcgcgagcaa
    27181 ggcattctgc ctctcgatgc gctggcgccg atgatgaccg tggcgcgccg gttcgagcgc
    27241 gtttccgacc aggcaacgaa catctgtgaa gaggcgctgt attttgccac gggtgagtat
    27301 atgcggcact tcccgagcgg cggcttccgc gtggtatttg tggatgagac ccatgggtgc
    27361 ctgagccgca tggccgagtt gatcggcaat aggctgggtg cggaacggtt ttccttttcg
    27421 agcgccggag tcgaagccgg cactctggat ccgcagacgg tctggtttct cgcggagaag
    27481 ggcttcgacg cttcccacga aaaatctctg tccatcgaac agataccaca actcgaacac
    27541 gtgcagatgg tcatcctgct ctcgaaagag gctgaggccg ttataccgcg agccccttca
    27601 aaagcgctga acgtgcggtg gctggttgcc gatcctacgc gagtgcacgg cacgccggac
    27661 gaaatacgcg cggcatacga acacgcgttc gaggcgctga ccacccatat ccgagatctg
    27721 attcaagctg tccttggaaa tcacggaaat ccgagtgatg agaacacgaa ttctacgatc
    27781 tgatacagcg gtgatgagat tcgcctcgtg cgcgctggcg gccgttgcgc ttgcatttct
    27841 ggctgtcggc tgcgcgcgat cgaagcaacc cgagaagacg gttatccaga acacgggttc
    27901 cgacaccatg gtgaatctcg cccaggcctg ggcggaggaa tacgccgccg ttaacccgac
    27961 ggtctcggtg gaggtgtccg gcggtggttc cggcacagga attgcagcgc tgatcaatgg
    28021 cacggtcgac atcgccaatt gcagccgccg cgttgaactg caggagatcg aacaggcaaa
    28081 gagcaacacc aaaaaagagc ccagggagtt catggtcggc tatgacgcaa tggccgtgta
    28141 catccacaag agcaacccgc tcgacgagat cacgctcgac cagttggccg agatctacag
    28201 ggaagggggc aaaatcaccc ggtggtcgca attgggagcc aagatgcctc cggaagccga
    28261 cgagatcatc ctgctcagcc gccagtcaaa ctccggcacc tattactact tccgcgaggc
    28321 ggtgcttggc aaggggcacg acttcaaact cggctcgcgc gacatgcacg gctccaagga
    28381 cgtcgtggag ttggtgtcca agacgcccgc ggcaatcgga tatagcggaa tgggctacgc
    28441 gactccagac gtcaaaatgc tgcgtgtcgc gaagaaagcg ggtgacaagg catatgcgcc
    28501 gacggtcgag aacacactga accacaccta tccgattgcc cggccgcttt tcatgtacac
    28561 cttgggagaa ccttccggcg ccgtgaaaac gtatctcgac tggatccact cggaggccgg
    28621 acagaaaatc gtggtccaaa gcggctacgt gccactctcc caaccgggcg cgccacgctg
    28681 acgcatgtcg acggcgggct cgtccacccc aatccggcgg cgatggcagg ccggcacaga
    28741 aactgccatc gaagcgctga tccatgtttg cggcatcagc gcgatcgtct ttgtcttcgg
    28801 catcttcttt ttcgtgtttc gagaaggagc cggaatcctg tttcgctcat tcagctttcg
    28861 cgagttcttc accagcaccg aatggtatcc gacctctcag agccacgtgc gttacggcgt
    28921 gctggcgctg attgcaggca cagccagcgt caccgcgctg gcgatggcgc tcgcggtgcc
    28981 gttcggcatc ggagcggcgg tgttcatctc cgaattctgc gggcctcgtt tgagggagtc
    29041 actgaagatc gtcatcgaac tgcttgcggc catcccgtcg gtggtttggg ggttcatcgg
    29101 attgacggtc atgaaccagc tcatcatggc agtgttcgac gcgccgatcg gcctgaacgt
    29161 cctcaacggc ggtgtgattt tggcgctgat gagtgtgccc atcatcgttt cgatcgggga
    29221 ggacgcgctc aaagcggttc ccgactctta ccgcgaggcg gcgctcgctc tcggcgtcac
    29281 gcgctggcaa cttgtatacc gggtactgct gccggcggcc aagaacggcc tgctcgccgc
    29341 ggttctgctc ggcgtgggcc gcgccgtcgg ggagacgatg gcggtgctca tggccactgg
    29401 acacgcggtc aaggttccaa caagcctgat ggattccgtc cggacgctga ccgcgacgat
    29461 cgccgccgag ctaggtgagg cgccggtaaa ctccgatcat taccaggtgc tctttgtgat
    29521 cggcatcctc ttgttcatga tcactttcgt ggtcaacctg acggccgatc tggtcgtgcg
    29581 ggggattcga aacaagtgaa aagttctgtc ttttgcgggt gactcaaggt gttccaggcg
    29641 actccattca cgcggcgtaa gcaggcaagc gagcttctcg cgaagtcgat cttcctgctg
    29701 atgacgttgc tgatggtcct cccccttatt ctgatcgtcg gatatctggt gtaccaagcc
    29761 tggccgatcc tgtcactcga ttttctcctt acgaatccga gacacggaat gcgggctggc
    29821 ggcatctggt caccgctgct cgggacaatc tatctcgtcg tgatctcgct gatcattgcc
    29881 acaccgatcg gtgtgctggc cgcggtcttc ctgaacgaat acgcgcgtga cacctggttc
    29941 acccgaatgg tcaaccttgc ggtcgtcaat ctggcaggcg tccccagcat cgtccacgcc
    30001 ctgttcggtc tcggggcgtt cgtgcttgcc gccggactgg gcaagtcgat cctggctgca
    30061 tcgttgaccc tggcaatcat gacgctgccg gttatcatcg cgagcaccaa ggaagctctg
    30121 gcagccgtcc caatggcgtt ccgcgaggcg tgctggaacg tcggtgcctc ccgctggcag
    30181 acgatccgcc atgtcgtgct tccgaactcg atcagcggaa tcctgacggg cgtgattctc
    30241 caggtctcgc gcgctgccgg cgagactgct ccggtgatgt tcaccggtgt ggtgtttttt
    30301 aaggcgatcc gggaaggcga cctgttcgca tacggtccgt tcgaccagtg catggcgctg
    30361 tccatgcacc tgttcaccat ttccacgcaa gtgccggacg tacccaaagc cttgccttac
    30421 ggaacggcgg tagtgctgct gggcactgtt ctcgcggtga acgcgctggc gatcctgatt
    30481 cgagtccgcc tgcgctcgcg gaggaaatgg tgacccccgg cccgatggtc gaactggcgg
    30541 acctgcgggt tcgttacggg cagcgcgagg ccctcaaagg cgtcaccttc gaaatgcgcc
    30601 ggaacgaggt gttcggcatt atcggcccgt cgcagtcggg gaagaccacg ctgctgaagg
    30661 tgatcaaccg gacgttggac cgcgttcccg gggtcatgat cagcggcacg gtgatggtgg
    30721 atggcgtgaa cattcaccgg atgaaggacg tgtactcgtt gcggcgcaag gtcggcatgg
    30781 tcgcgccgct gcccgtcggg ctgccgctgt cgatcttcga caacgtggcc ttcgcgccgc
    30841 ggatggccgg cattcgggac aaggccgatc tcgaagcgcg cgtcgaacgg tgcttgcggc
    30901 aggcagccct gtgggacgaa gtcaaggacc ggctcgacag cctcggaacg cggctctccg
    30961 gcgggcagca gcaacggctg acaatcgccc gcgccctctc gcacgacccg gaggtgctct
    31021 gcctggacga gttctcgatc gccgttgatc cggtcacgac gatgcggatc gaggatgtcc
    31081 tgaaagagct gcggtcccgg atgacaatca ttctggtcac gaatctggtg cagcaggcgc
    31141 gccggctggc tgaccggacc gcttttttcc tgaacggtga actggtggag accggcccgt
    31201 ccgaggtcat cttttccgac gcgccggcga atcgcaagac gtacgagtat gtgaacggga
    31261 ttttcggatg acacctccgg cggccgcgac ctccttcagc atcgagacca ggaagctgag
    31321 tctctggtac gggagtttcc aggcgctgaa ggatgtgtcc atcacgatcc gccaaggcga
    31381 aattaccggt ctgatcggcc cgtcgggttg cggcaagacg acgctactgc gctgttttaa
    31441 tcgcatcaac gagcgttacg gctatgtacg gacgacgggc gaaattagaa ttctcgggaa
    31501 gaacatctac gatacggatg tctcgctgat cgagctgcgc aaggaagtcg gcatggtgtt
    31561 ccagcgcccc aacccgttgc cgatctccgt gtacgacaac gtcgttttcg gccttcgcgt
    31621 ccacagcggc cttggcagcc tgaggaaatc agttcttgac gaagcggtgg aaaaagcgtt
    31681 gcgcgaggtt tccctgtggg acgacttgaa agaccggtta ggggtccgcg ccacgacgtt
    31741 gcaactggat cagcagcaga aactctgcat cgcccgcctt ctccctttga aacccgagat
    31801 catcttgatg gatgagccat gctcagcgct ggacagtgaa gcgacgctcg gcatcgaaga
    31861 gctcatgctg tcgctggccg gcaggtacac gatcctgatc gtcactcaca acatggctca
    31921 ggcgcgccgg gtcagcgacg aatgcgcgtt catgctgctc ggtgaaatcg tggaacatgc
    31981 gcgcaccgag gatctatacc tcactcctag agacccgcgc accgccgact acattgaagg
    32041 acgctacggg tagggtcaag tgaacgagcg cgaccattcc ggcccacgcg aagcgccggc
    32101 gccggttctg ctgatggtac gcgaactcgg accggggggg agcgaacggc aactcgctgt
    32161 gatcgcccgc gcgctggatc gggaacagtt cgagccacac gtcggctgct tccggccgga
    32221 cggaattcgc ggagacgaac tgcgcgccgc cggcatcccc atactcgaag tccctgtgca
    32281 ttcttttgcc tccaccaggg cgttcggagc ggtgcggccg ttcgcacgct acatccacga
    32341 acgcggcatc cggctggtgc acacattcga cgagccgatg aacgtttggg gtgtcccggc
    32401 ggcgcgcgta gctggcgtgg gcgtggtgct gtcaagccag cgcgcgcatc gagagcttgt
    32461 gccgcccctg cgccgccata tgctccgctt cacagaccat ctgaccgacg gcgtggtggt
    32521 gaactgcctg gctgtgcgac gtcacctcat cgaggacgaa aaggtttccc cgtccctcat
    32581 ccatctctgc tacaacggca tcgacacggc cgcgttcgca cccgaacggc gacggcctga
    32641 ctcgctcgcg gccgccggcc tcaccatcgg ctgcgtctgc gctctccgtc ccgagaagga
    32701 tctgacgacg ctcgcacggg cctttgccgc cgtccgccac ttgcatccag acatgaggct
    32761 cgttatcgtc ggctcggggc cctccttgcc cctgctgcga gactgctgcc attcgctcga
    32821 cctcgaaaac cagtgcgttt tcgagcccgc caccagccgc gtggcggagt ggctcggagg
    32881 gatcgacatc ttcgtcctgc cgtcgctctc cgaggcgttg tcgaacgcac tcatggaggc
    32941 catggcctgc ggctgttccc cggtggccac ccgcgtcggc ggcaatccgg aactggttga
    33001 gcacggccgg accggttttc tgttttcgcc cggcgaaccg gaggaactgg ctgccgtgct
    33061 tcgcacgctg atcgaaaatg acccactccg gcgcgagctc gcctgtgcgg cgagcgcgaa
    33121 ggtcagggac aacttctcga tcgaggtggc cgcgcggcgc atgggcgaga tctaccgcca
    33181 ttttctcgac cggcgtgggg accgctgaga cggggagact ccgggccctg cgggtcgact
    33241 aagatcgaaa catgaacgaa cttcgaggca tcaaccgccg tcgcgctctg accgctctcg
    33301 gagcagtccc gatcgctggc ctagctccgc agacgagggc ggcggaagac ggtgcggcgg
    33361 cgccgtccga caaaccgcgc gagttggcgc cgatggaagc ggacatgggc accctatacc
    33421 cggacatcga gcgcctggtc gcggcgaatc ggccgtcgca ttcctttctt gacagccgtt
    33481 tccagtccct cgaagactgg aagcggcggg ggcgtgagct tgcctgggag tcgttcggtt
    33541 acaggcccgc caccgtggac ctcaacccaa aagtggtcga ccgtcaggat ctgggggaat
    33601 tcgtgcgtga gaaggtcctc ttctccacca cgccgcaatt ccgcgtggcg gcctacgtac
    33661 acatcccaaa gaaccggacc ggccgcggac cagccatcgt tgacctgcac tcccacggag
    33721 gaatgtttct tttcgggaag gagaaagtaa tcgactttgg ccgcaatcac ccggcgatga
    33781 ccgagtatca caaaggcaac tacggcggcc gcccgaccac caccgcgctc gtgcgccgcg
    33841 gctacgtggt tgtcacgata gacgcgttca tgttcggcga gcgccgtctc atcatggatg
    33901 aagacttgaa atacggctgg gagcgctccc ggtattcggt ggaggacgtg aagcatctga
    33961 atgctcgctg cgcttccaag gaggacaccc tcgctaagtc gcttacgctc gccggcatcg
    34021 cgtggaacgg cgtcgtggcg tgggacgaca tgcgcacggt cgactatctg ctcacgcgtc
    34081 cggaggtaga tccaaagcga atcggatgtg tgggagtctc cttcggcggg tatcgtaccc
    34141 tgttcctcgc cggcctggac gagcgcatcg ccgcggcttg tgtggtgggc ttcatgtcca
    34201 ccatcaagcc gatggcccgg agacacatcg acacgcattc ctgggttcac ttcgtgccgt
    34261 cgctgcaccg ctacatggac tggcccgacg tcgtgggcat gatggccccg aaaccgctga
    34321 tggtgcagca gtgcaggcag gacggcctgt ttccgctcac cggcatgcgg gaatcactgg
    34381 aaaggatcgc agccatttac gaaaaggcag gcgtgaagag ccactttgat ggccgctttt
    34441 atgatggtgg gcatcgcttc gacgtcccga tgcaggagga cgccttcgac tggttcgacc
    34501 gccacctgaa ggcttgacca ccggaaactc tattgaaagc agcttaccga attactgata
    34561 aatgactgat aatggttcca gcggtgctgg aggcggaacc cccactgcat tacttaagca
    34621 aggaagaagt ctcgaatgac cagtcgattt gccgcgatcg catttgtttt cgcggctgtt
    34681 gcgccggtgt gtcccagcca ggaggtggcg cctccacggc caagggcagg aaaagtcgag
    34741 atcctcaagt cggccgcatt gaagcccgga atgaaaggcg tcgcctggac cgtgtttcag
    34801 ggcagcgagc cggagcccgt cccggtagag atcatcggac tgtggaagaa cgcttgggga
    34861 cccaggcagg acgtgatcct ggccaaactg ggtggcaagg tccagcgcac gaacgttgcc
    34921 ggcggcatga gcggttctcc cgtctacatc gacggcaagc tgatcggcgc tattgcgttg
    34981 cggcttagcg tgttttcccc ggacgcgatc tgcggcatca cgccgatcga gttgatgttg
    35041 gagatatccg atttcgactc caccgtcccg tccggtgcac gaactccgga caaagctgtc
    35101 gtgcgcgacc ctctggccgt gccgggggag gtgctcgcac aggctgtggc cgccggagcc
    35161 agccccggcc tgctccagca aagtcctctg atggtaccga tcgaaacgcc gctgtctttc
    35221 tccgggttcc atgaaaacgt gctgcgggag ttcggccccc tctttcagca gttgggcctc
    35281 accgccgtgc agggcggcgc cagcgggagc ctgggcggcc cgaagccagc tcccggctgg
    35341 cagaacgcgc tccagccggg cgaccccgtc gcgggggtgc tcgtgtcggg cgatatgacg
    35401 atctccggac tcggtacagt cacctacaac gacggcaaac gcgtgctggc cttcggacac
    35461 ccattcttca acctgggccc ggtcgagatg cccctcagca agggtgaggt gctcatggtg
    35521 ttgtcgtcga gcttccagcc aaacaagatc gccaacgcca ccgaagtcgt cggctctctc
    35581 aagcaggacc gccatagcgg cattctcgga gtgctcggcg agactgcgga catgatcccg
    35641 gtcagcgtga aggttcgatc gttcgccggc gacaactcgg ttctcaagga aaaagccttc
    35701 cgttacaacg tcttcgtgca gcagcgttgg acgcccaacc tgatgatgat cacgctgttc
    35761 aactcgatct ctggcgtcaa tgatttcagt gacgagaaca cgtaccgcct gagcggcaaa
    35821 gtggaactgg acggtggcca aagcctgtcg ctcgaaacca tgcaggcgcc gagcgaggta
    35881 ccgattcccg cgccgattct ccttgccggc tgggttggtg acaagttcaa ccggttgttt
    35941 ctgaacgcgg tgaaagcgcc gcggctgaag cgcgtggacg taaacgtcga tctgctgccg
    36001 cagcgccgca tcgcagccat cgagagtgcg tgggccggcc tcagcgaggt cgacgccggc
    36061 ggtgaagtgc cggtcaaggt gttcctgcgg ccgtaccggg gggagaggat cgagagagca
    36121 ttcaacctga agattcccgc cgggcttcca aagggcgagc acggtattct gctaagcgac
    36181 gccgagacgc tcaaccgcct taagggcgcc gtgggccgtg cgaaccggtt cattgacctg
    36241 gcgcagacag tctcgctcat caaccaggag cgcacgaaca accagcttta cgtttcgctc
    36301 gtgcaggcga gcccgaccgt ctattacgat gacaagacgc tcccgagcct acccgcatcc
    36361 gtgctcaacg tcatgcaggc cggcaatgca gcgaaccgtc cgtttatcac atcccgggag
    36421 acggtcctcg aacagacggc gatcccgttc gattacgtga tcgacggcag ctacgcgctc
    36481 aaaatcaagg tcaagtaacc actacccgat gcaaagaatc cgctttttcc gcaccgtcgc
    36541 ggccgccggc gccatctgtt gcctggcggg cgcggtcgag accagatcct ggcagcagga
    36601 cgcctacgaa gacttcgaca agggacacat cgcgaaaatc tcgttgcgca gcgacggcca
    36661 gctgatgctc gctcctgttt tcaaggaact gctcgattct ccgtccccgt atctctgggc
    36721 gctggccgaa gactcgaagg gcaatctttt cgcgggcggt ggaggtcccg gaggcgcggg
    36781 agcccaagtc tatgccatct ctcccgacgg caagagccgg acactggcga agctggaagg
    36841 cctggaagtt cacgccctcg ccgtcgataa gcgggaccgg ctctatgccg ctacttcgcc
    36901 cgacggcaag atctatcgga tctcctccgg cggtgcggct gagacagtct acagccccgg
    36961 tgcgaaatat atctgggcga ttgccttcga tagtaagggc aacctgtttg ccgccaccgg
    37021 ggacgaaggt cagattcatc gggtgacgcc ggatggcaag ggcgccgtct tctatcggac
    37081 cgaagagacg cacgcgagat cgttggcggt ggatgagaac gacaacctga tcgtcggcac
    37141 ggacccgggc ggattgatcc tgcggatcac gccggcggga gagggatttg tgcttcacca
    37201 ggctcccaag cgcgagatca cagcggttgc cgtcgccaag aacggctcca tctatgccgc
    37261 ggcgacggga gtaaaacagc ccacgatggc tccaccccca ccgctaccga aaccggtcgt
    37321 ttcgccagtg aagcccgccg gaccaccgag ccccgggcaa gtccaaccat cgcccacccc
    37381 gccagcgaat ctgccgtccg tcgccacggc cgcggaaggc tccgaagtgt accggatcga
    37441 tccggacggc tatccgcgcc gcgtctggag ccactccaag gatctcgtgt acgctatcgt
    37501 tttcgattcc agtgagcgtc ccttggtcgc gacgggcaac aagggcatcg tctaccgctt
    37561 cgattccgat cacgtctata cggctctcgt cagcgtgcag cccacccagg tgaccgccct
    37621 atgcgccggg ctgaagggca aagtgtacgc ggcgaccggg aacattggta aggttttcca
    37681 gatcggtccg ggtcttgaga aagaaggttt tgtcgagagc gacgtactcg atgccgagat
    37741 cttctcgctc tggggacggc tggcgtaccg cggtgacctg aacggtggtg acatccgcgt
    37801 ggagacgcgg agcggaaacc ttgaccgccc gcgcaaagac tggagcccat ggcagccggt
    37861 ggggctgggt gaagacggcg gccggatcca atcgccgcag gcacggtttc tgcaatggaa
    37921 gctgaccctc acctcggcat cggatgggca ttccccggag attcgctcgg tcgacgtcgc
    37981 ctatctgccg aagaacgtcg cgcccgtggt tgagcagatc gagatcacgc cgccgaatta
    38041 tcgcttcccg ccgcaatcgc tgagtctcac ccagacgcaa accctaacgt tgcaaccgct
    38101 gggccgcgcc cggaggggtt ccccgcctcc aggcctcatt gactcagggg tcctctcgat
    38161 gcactacgcc aagggctaca tcggagcgcg atggaccgcc gacgacgaga acggcgacga
    38221 gatgctctac gacgtcgcga tccgcggcgc cagcgagacc gattggaaac tcctcaagga
    38281 gaagatcaaa gagaaatacc tgagttggga ctcgaccgcc tttccggacg gcgagtactg
    38341 gctgcgagtc actgcctccg acacgcccga caaccctccc gaccaggccc tgagcgccca
    38401 actggtgagc gagcgtttcg cgatcgacaa ctcgccgccc gagatctccg gcctggcggc
    38461 ttcacaatcc ggcggcaagc tggaggtcca ctggaaggcg cgcgacgtgc tcagcgtgat
    38521 cgggaaagcc gaatactcgg taaacggtgg cgagtggacg ttcgtcgagc ccgtgacgaa
    38581 gctatccgat tcgaaggagc tcgactaccg cctcgtcctc gacaagctct cgcccggcga
    38641 gaagacaatc gcggttcgcg tcgccgacga attcgacaac ctcgcggtcg ccaaggccgt
    38701 cgtgaagtag gcagcgcccc cttaggacga gggctttccc acgatcgcct ccaccttcgg
    38761 ggccagttcc tggtaggaca tctcgccggg gtggtagaga cgcacgatgc cgtgccggtc
    38821 gagcagcacc agcgtgggag tcgtactggc gccgtagttc cggaaactct cttcgccgac
    38881 cggcactggc atctcggaga gctccgcgta gaaccgccgg cgcacatcgt cgatgtaatt
    38941 caactcgacc tcaggggtca cttccatccc acgcgcgacg tagccgtacc gctgcgtcgg
    39001 accgacgagc gccaggcctc gcggcccgta ctctttgatc agccgcgcga gaacgggcgc
    39061 ctgccccttg cagtcgccgc accagtgcgc ccagaagaac aacagcaccg gccgtccctt
    39121 gagttgatca aacgattccg gcttgggtcc gagccacact gcggtctcga gcggcggcgc
    39181 ggatttcccc tccaagctca gaaggtgaat gttcttctgg atccgcgtcc gaatggacgt
    39241 accccggtac ctgtccagag cgcggtttag aaattcaacc gccccgctgc gctcaccgcg
    39301 ggccgccatc acctgcgcct gagtctcgat ggccgccccg agcgcgaccg gaaggtaccg
    39361 gtcggtgtcg agagcgcggc tcttcagcag gctctcgccc actcggagca cctcggcggc
    39421 atggacctcc gcttcgtccg tcttctttgc ctccagagcg ccccggccaa gccaggagag
    39481 cgccgcgagc gcctccggcg tgtcgccttt ctcagcacga tatctccgaa ccagcacctc
    39541 cgcaccggcg aagtcgtcac gtgcaatcgc cgtgcgaacc tcgcggacta cgttctgcgc
    39601 ggttgcaggt tcaagcagga acaaaaatag cagcggaagg agattggctt tcatatcatg
    39661 agcagcttct gacaatggcg gatactcgct tccagatcct cccgctcgcg ctgggcggcc
    39721 tcttctttcg gagcgggcgg cagtccttcg tgaggcttgc cgcgctccgc aagggcgagg
    39781 aaacgggaga actcccacgc acggacgttg cggtaaccct cccagaactc cggatcgagg
    39841 taggaaaaga cgcgcggctg cgtcaccaga acctccaggg agagcgcccg gcccgggcac
    39901 aattcgagaa acttcctcac gtacccgtcc agatcgacat tgccctctcc catccggacc
    39961 cacttgaccg cgacgccctc tggtacgcgc cagatcgcgc tgtcgcgcac atgactcgtg
    40021 accgagtagg ggtgcagcac ctcgagtgtc aggtgcggat cctccaaggt ccagagcgga
    40081 ttgccggagt cgatgcaggc gccgacgaag tccttacccg cctcttcgat cagcattctg
    40141 agctcgcgcg cctgcatgtc gccggcgtga ttctcgaccg caattctgac gcccgcgtcg
    40201 agcacccgag accgcacagc ctttagcaca cggacggtgt tctcgatgtg cccctcgatc
    40261 ggaatcgaac cggtacggtc ggccattgtg ccgagaaatg ctcgcaccag gcgtgccccg
    40321 gtcgtctgcg ccgccttgac catgcgcatc acctgctcct cggccgtgcc ctgagcgggg
    40381 tcgaacgctt ttgacgtcgg gcaaatcgag cgcatgccga tctcgagttc gatgccgagc
    40441 cggtccgcat gcgcacgcac tttcttgagg tgcgactcct cgaggctccc gagaaaccgg
    40501 atctcggaga agtggacgac gttcgccttc cattttgcgc agtaatcgag gtattcgaaa
    40561 ggcgaccagc cctgcgagcg gatgctgaag agatcgattc caaatttcac cacgggcttc
    40621 ccctgaaatg agggttgcgc ggccattgcc actccggttg ctgcaagaaa actccttcgg
    40681 tccatcgcgt cctccggccg gcctagagtt ccaggccctt caagtattcg cggaactccc
    40741 cgcccaggtc tttgcgccgg agcgcgaatt cgaccgtggc cttgaggaat cccagcttgt
    40801 cgccagcgtc gtaacgcgca ccctgaaacc ggtagccgta gatcggtcgg ctgcacaaga
    40861 ggtgcttcag tgcgtcggta agctggatct cgccgccgct cccgggttcg atcgcctcga
    40921 tggagtcgaa gatctcggga gtcagcacgt aacgtccgat aatagccagg ttcgagggcg
    40981 cttctgaagg tttcggtttc tccaccaggt tgcggatgcg gaacacgcgg tcctcttcgc
    41041 cgttgtgtga gacaggctcc gcttccacca ctccgtaagc ggagatgttg tcggacggca
    41101 cttccatcag agccagtacc gacgcgccga aaaactcata aacatcgagc agttgccgca
    41161 agcacggcac ttcggagtag atcacgtcgt cggagagcac cacggccagc ggttccctgc
    41221 ccaccaattc acgcgcccgc aacaccgcgt gcccgagccc gagggcctct ttctgccgca
    41281 cgtaggcaac gttgatcatg tcggagatgt cgcgcacgat ggccagcgtc tccgtcttgt
    41341 tgcgcgattc gagcaggtgc tcgagctcga agctcacgtc aaaatggtcc tcgatcgccg
    41401 acttcccgcg cccggtcacg atgataatgt tctggatgcc ggaccggagc gcctcctcca
    41461 cgccgtattg gatcagtggc ttgtcgacca ggggaagcat ctccttgggt tgcgccttcg
    41521 tcgccggaag aaaccgcgtg cccagcccgg ctgccgggaa gacggccttg cgcaccttag
    41581 ggaccatgaa gactattgta aggcccggtc cgctgtggaa actcccaccg ccccatgcta
    41641 tacatggtat ttctcagaga agagggccgc tccaagcggg gagaggggat atcaatggct
    41701 tacgtgattg ccgagccttg catcggcacc aaggacaccg cctgcgttga cgcatgcccg
    41761 gtggactgca ttcacccaaa gaaagatgag gcgaacttcg aaaaggaaga gatgctctac
    41821 atcgacccgg tcgagtgcat cgattgtggg gcctgcgtac cggtctgtcc ggtgtcggcg
    41881 atcttcgctc tcgatgacct gccggagaag tgggcggaat tcaccgcgcg aaacgcagcc
    41941 tattacggcc ggtaggtggc cgtcgaggta tcggtctccc agagcttcac agccgcgacg
    42001 cgaactggcg tcgccggttc ctgcgaggcc aatcccttgc tcagctcatc atagaagtac
    42061 ttcgccatgt tctcggctga aggattctgg acgtcgaacg gcggcacttc gttgaggaac
    42121 tggtggtcca ggcgttcgac ggccgcgtgc agaactcgtt tcacgtcaac gaaatccacc
    42181 aacaagccga tcgaattgag ctcctcgccc tcaatcgtga ccagaacgcg gtaattgtgt
    42241 ccgtgcaggt tctcgcattt cccgcggtaa cctcgaaggg cgtgcccagc cgcgaacgtt
    42301 tcttccaccg agatctcaaa caccgaagaa cttctccact tcgctcaggt ccgtcgtgaa
    42361 tctgtagtcc ggcaggctat cgaagaacac ctgcccgtaa cgctgcctcg tgatccggtt
    42421 atccagcagc gcgagtacgc cccgatcggt ccggctgcgg atcagccgtc cgaaaccctg
    42481 cttcagcgaa agggcggcct gcgggatctg atagtcgtag aaagggttac ctccctcttc
    42541 gcggatgctc cgaatcctcg cttcgaccac cgggtcgctc ggaaccgcaa acggcagctt
    42601 atctatgata acgcaactca gttgctctcc cggcacgtcc acgccctgcc agaaagacgc
    42661 agttgcgaac agcacgcagt ggggggtggc ccgaaactcg tcgagcagcg ccgtcctcgg
    42721 tccggtgcct tgaagcagcg tcggatattc ggtgtcgaag gagatgcggt cgtagacgac
    42781 gcgca
//
LOCUS       DATIFQ010000343        79698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_498118, whole genome shotgun sequence.
ACCESSION   DATIFQ010000343 DATIFQ010000000
VERSION     DATIFQ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 79698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79698
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498118"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VLE22_16035"
     CDS             <1..1229
                     /locus_tag="VLE22_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15966.1"
                     /translation="FDRINPLHIVLYYPDGLEAVSPGKLDLETSYYFEIDPNRAGNVF
                     SRSEWDNEQASWFSFVTRYSNANHTHYDMNSFLFTAFGEAFATHANIFPYSHKHHGVD
                     FEHNIVIVDEGGMPANDRQSAGDDGSVKGFMTGLGLGHFADYVRGDARLSYADRSIRT
                     STPALHANRSVLFAKQGPNPYVVITDEIQKDTGEHDYHWQWYTEAKAIAGQGTFDEPF
                     VIEGENARCRIAFLDPAAPSHDFRIAKGGSERRPLELGLLRVNLRGVQARYVAIAAAH
                     RKDAPVPRMSRGAAVEGNPSAASLIVEGEAFRDVIVWQPEEAGGSRGRPISAGKLKTD
                     ALLAMVRTDQKGKVIGYLMADGASLVFSGKALARADAPFSVSADAKQTVTTGARRARE
                     GLPPLPAPRATLVKLR"
     gene            complement(1622..3739)
                     /locus_tag="VLE22_16040"
     CDS             complement(1622..3739)
                     /locus_tag="VLE22_16040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HSB15967.1"
                     /translation="MRYGVRQSSKMAILESTSHSAGDSLAIKHLLALLTLGLCLGPTV
                     HAALAAGLTSADLYRLRSVGEIRLSPDGAYVAYTVSHRDGGGRPYEQVQVMPLNGGQT
                     VVLAKGKDESSMVEWSRDGRWLAYSGKLGEKAGLILCRPDGSGARWIAPLEWTNSPMQ
                     EPGHRLAWAPDGKQIAFVSTLPGPETAEASGDPVVITRYLYKPDYTEGLTHFNDNRRS
                     HIFIVDLESGKVRQLTTGNHYEHSIDWSPDGREILFVSNPSPDEDLFFNSDVFAVKAA
                     GGTIRRITATESAEYVPRWSPDGKQIAFLGTRRGLTDLETTMEDTHVWVMNSDGSGRR
                     EVGTVVDNRQGPPEWTPDGEFVYFTVQERGSVLLCRIPAAGGPAERVIAERGAVRSWS
                     LTAGGLLSYAFTSSHDEVELFLRKGDVTKQLTDLNADVLRGKEIAPVERFTFVSNDFK
                     FEVEGFLTKPLGMTGTSRHPLIVVIHGGPHGQQGPGFNFHNQVYAAQGWATLMVNYRG
                     STGYGQKFADGVFGDQNGNEAQDVLYGVNAATRRYFWLDRDRMGIEGGSYGGQLTAWL
                     VTQTNIFKAAIPLAPIINLVSYNYTTYYNMYEEMEFGAKVHQGNLMDVLWERSALKHV
                     AQVRTPVMLLHGENDADVPITESEQFYIALKDVGVDAVMVRYPREGHGLREPKHIVDG
                     IDRSIAWYRKYFPRETVSEAAGR"
     gene            3969..4349
                     /locus_tag="VLE22_16045"
     CDS             3969..4349
                     /locus_tag="VLE22_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15968.1"
                     /translation="MTEIEAIRNMVPYGERPLFDKLVEELPLVLHDKKNHSPGVQAAW
                     VARAVVLALPMDCSRRTDENCCGGCLYWRKSSSAGAFGLCDRISFGGHVHGDVLACPV
                     DTRDDPASLRTLPIFGCVMFKVRS"
     gene            5566..6588
                     /locus_tag="VLE22_16050"
     CDS             5566..6588
                     /locus_tag="VLE22_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15969.1"
                     /translation="MTRRGALILVGIALLAVAAPRRRPAMPKITQPVLFGTPEADRIL
                     AALQVFPPDNPWNQDISTLPVHADSARIIASIGAGKTLGYNLDMNFVIVPPNQKRIPV
                     KMLQYPAESDPGPFPVPDNGPIENWPLAVNEDIKAVARPGQTLDDIQRHGRGDRHMLV
                     VDPGNGKLHEFYAARKTDAGWEASNVATFDLRTGALRPEGWTSSDAAGLPVFPATVRY
                     DECERGLVEHAMRVTVRRSRRAYVLPATHFASRLTDPDLPRMGERLRLRRDFDTSGFP
                     PHARAILEGLKRYGMFVADNGQDWLMSISPDARLKGLETLKRVKGSDFEVVETGAHSG
                     SERPAK"
     gene            complement(6746..6820)
                     /locus_tag="VLE22_16055"
     tRNA            complement(6746..6820)
                     /locus_tag="VLE22_16055"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6785..6787),aa:Glu,seq:ctc)
     gene            6882..8009
                     /locus_tag="VLE22_16060"
     CDS             6882..8009
                     /locus_tag="VLE22_16060"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSB15970.1"
                     /translation="MKWPLFALLTMLAAALAWSGVRSPEIPFEKVTIDLGAAETCAFT
                     DINGDGRLDIVSGEYWYEAPKWTPHKFRDLPYSNNYMDAFSDLPLDVDGDGRPDIATV
                     AWFGRKINWFRNPGPTGGAWREQAIEDGFNVEFAFLADLDNDGKAREILPQFGNENAP
                     LAWYEARNGAFVRRIVAPKSQGHGIGAGDVNGDKRNDVVTPKGWYEAPPDPRTGEWTF
                     HPDFDLGSTGFIHVFDVNGDGRNDLVTSLAHDYGIFWMERTAGGQWKKNLIDESWSQA
                     HALVLTDLNGDGRTDLLTGKRYMAHNGKDPGEREPLGLYWYEYRLSNGGNVEWIRHVI
                     DYSTRAGGGMQIAVADIDEDGDLDFAAGGKSGLFLFLNRTR"
     gene            complement(8072..9727)
                     /locus_tag="VLE22_16065"
     CDS             complement(8072..9727)
                     /locus_tag="VLE22_16065"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB15971.1"
                     /translation="MSQRKMPANQPQSPGTGAVGNVAEAKSERRGASHALAKAVSLHL
                     EGKPQEALNELYSAVDSGEKDAELYSALGHIHFQLEQYEEATTSYWKLLQLEPSHRTA
                     SFNLAVCLEKQGRWQEAVERFQRSLEVDPGRNEAYLGMGICNLQLGQLEPALEAFDRC
                     LNQMPTQEAAMFGKAVALQLAGRFDEAGDQYRRILQRNPTSEDSLVNLIAVDMARKDS
                     DRVRETAEQLLSVRPNSQTALEGLAASAFSGGDHESAARYCAKIVEIAPNHFEAWFNL
                     GVAHQKAGRFHDAARSYSEAARLRPDSPQVHVNLGIVQQELGDLPSARKAYERALQLD
                     PGQTAASWNLALVLELSGDPDTAEALYVKLLDGNPEWVDARFRMGYLRLQRGDFQGSA
                     DAFDACLKQRGRWPEALLNLGIAYWKLGRTDDARKAFDQVLAMQPDSIDALRGLAAMA
                     LESDDYQAALQLHGRLIELGERGPELFYNTGLLMQKAGIMDEAVRLYREALAVQPEFA
                     EAALNLGHALKALGQEDEARSFWRRALEIKPELAHGYFEPVVA"
     gene            complement(9724..12204)
                     /locus_tag="VLE22_16070"
     CDS             complement(9724..12204)
                     /locus_tag="VLE22_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB15972.1"
                     /translation="MHCLYCGKALGFLKELTDGEFCCGQHRQRYKKLTKMALARLQET
                     KTDLAREVFPLEPEINNAVQSARSAVSSIFFPSDGRKPRDYSNVLRHFPRPQQIPAHL
                     QAADETMQGVPGPSLPGFSHSPILPLLGNAAPIWGRLDPVAPTAPGLALGAILKPSEL
                     PHPSPRFEFLSGMLRQGSTPTLREAVFSLPRPAPAQPSPSSATPDVASVAPIFELALA
                     VAMPPVPGPAVRPLLALAQPIPQTPNAATANSWAAVRQAELDPLPFALPVVWKHAAAL
                     HLAPLELPPAVELTSHAICERAFPVAPAAQPALAACRPDTTAEWRAWSMVPTLASARS
                     GIELIFSVTKAGFQPARLPVFEPAAAMIAVEAHPVPLAPALTLLPRLALSPVALPAEA
                     VARAAAAAAATQATAPDAGLASFPMAGLTPLAVMAAAPKYGASAGVGPAPFRPSMARF
                     PVVEPIPVRQALGTIGSRTEEVRALNAAAVPLDAPHGAVAAATSSVLPSLASLFRYSR
                     AGKVQRGTVINFDGVRPKDQTRVPLAYMELPMPLRTPAIPRTSSLRIVETFEYLRPLD
                     RPELDPWQAILRLWRNTPAYLRFATASFCVLMMLWVASPRRVGTVVEGHWANVRKSIQ
                     DRATIELVDNFEGGSLGGWAGSGNWLKSWTFDDSGFIRPGKLAIYSPSVEMQDYRIEF
                     LAQIDRKAVSWVFRAADGMNYYAAKLTVVKAGPAPQMALVHYPVVNGVEGEHTEVPIR
                     MLMHNNTPYRVQVAVRGRDFSTSIEGQLIDHWRDDRLEKGGVGFFSETGERARLYWVK
                     LSHQDDFVGRLCSYMYPNPIMLRSGNLP"
     gene            complement(12532..13674)
                     /locus_tag="VLE22_16075"
     CDS             complement(12532..13674)
                     /locus_tag="VLE22_16075"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB15973.1"
                     /translation="MRITAIETIHLGRGITVHAGPIQWLWVRIHTDSGLTGLGETYPR
                     PESEKAVIHRTLAPVLLDRDPSGIDRLWSDMFQAVAYSGWAGAEMRAISAVDIALWDL
                     AGKVAGAPIYQLLGGASRSAIRIYNTCYDHVDFMTEPVRLARELLDVGIRAMKIWPFD
                     PIAKETGGNFITADQLRRGLDPLRRIRDELGDSVDVAMEFHGYWNLPSAIRIAQALEP
                     YEPMWLEELLPQDNLAAYRELASATRLPLCLSERLMTRYGFRELFENRAARIVMPDIC
                     WCGGISEAKRIATLADTHYLPVAPHNCGGPVLHLASAHVAANLTNLAILETVRQHYAE
                     EYAGILTHSLVPAGGALPLPPGPGLGVDLNPSIFSRSDVQVDRTDC"
     gene            complement(13715..14224)
                     /locus_tag="VLE22_16080"
     CDS             complement(13715..14224)
                     /locus_tag="VLE22_16080"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HSB15974.1"
                     /translation="MLFQIGEKVVYPNHGVATIENISIRSFGTQAERFYLLRLMCSSL
                     TVMVPFSHVDEIGLRKVTRNGQVAKMLDFLACGKCKCPGDWKDRFKENSEKMRVGDMF
                     QVAEVLKSLLLLQNVKPLSFREKKMLDRARHMLITELSTSRGIPEASAIDMLQRALAK
                     AALALPPAL"
     gene            complement(14491..15360)
                     /locus_tag="VLE22_16085"
     CDS             complement(14491..15360)
                     /locus_tag="VLE22_16085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB15975.1"
                     /translation="MSISRRCFLQSSAVLCASPAVLAARPKPLGKLKIGVTDWNLRQT
                     SKLEALSLAAALGFDGVEVSLGRKPEGDRLPLSDAGLQQRYVAEAKSRKIGIAGTCLD
                     ILHVNYLKSDKLGQKWISDGILATKNLKARVMLLPFFGQGALKSHAEMDYVGDVLREL
                     APEAQKAGVVLALENTISAEDNVRIMDRARSKALLVYYDVGNSTNAGFDILKEMRWLG
                     KDRICQIHLKDGRNYLGEGQINFPAVMQTIADIGYTGFANLETSSPSGSIENDMRRNL
                     QYVRGLVEQIRKG"
     gene            15795..17102
                     /locus_tag="VLE22_16090"
     CDS             15795..17102
                     /locus_tag="VLE22_16090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB15976.1"
                     /translation="MSGLQTGSGLAGPDATFRLDVSLHDLLVKHPPKPAPPPPRTAEP
                     RALPDPVFPQHPELLGKEVEPPPRDPNSPYGRRWTPALIYRALNGWLFPYVKSRVLPG
                     DFHPIIAYLFTEWKCNLDCHYCWAFDNSVKGMSEDTARRSIDWLHSTTCRVLALMGGE
                     PLLRPQFAHKVVYYAAKKGFWVYVPTNARLLRPEVTDRLADAGMAVVNFAVDVVNEKP
                     GLPKALNPVREYFEYLISKQYQYGYIVFFNINICRNNLEDVQKLTEIARDYGIITDYH
                     INESPMIEQSHFQHMNENDTFIRREDWPKVDALVDWLIDKNRAGYKMVNSAKRLADMK
                     AFMRGKVEPWNCRAGQNSMIIRVDGTLAPCFPMYSATYDWGTVENHRFEVKQLDQMKE
                     SCQKHCFSTLNHNLGYCYNDARVLRWVTRQALRGFQGVSGSFD"
     gene            complement(17122..18123)
                     /locus_tag="VLE22_16095"
     CDS             complement(17122..18123)
                     /locus_tag="VLE22_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSB15977.1"
                     /translation="MELSKVGQPFTAWELLDLVREDLQLVEREIQLQSVASVDAITAI
                     GRYLHASGGKRLRPALLLLASKLFGNGSESAIRLAAVVEMIHAATLVHDDVIDVAETR
                     RGRPSTNVLWGNHISVLAGDWLYMQAFQVALRERNFHVLDVLIGLTQMMVEGELVQLG
                     RIGSVDVSEADYMDLVDRKTASLFSACARLGAVTGGADEADEARLGEYAWNLGMAFQL
                     VDDVLDFTADESVLGKPVGNDLREGKVTLPLIYALENASTEERSQVEEILRDGSYDRV
                     PFNGILEFMERRRSIVRTMERAEAFTAKSREIIRGFPESPYQRALFALTELVTDRDH"
     gene            complement(18138..18923)
                     /locus_tag="VLE22_16100"
     CDS             complement(18138..18923)
                     /locus_tag="VLE22_16100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSB15978.1"
                     /translation="MTLVDSHCHLDDPRFDGDRELVIERATNAGVEVILTVGTGEGPP
                     DLEAGLRVASTHERIFATVGVHPHDASKAAGDTWNRLRQLAAQPKVLAIGEIGLDYYY
                     DNSPREQQQAAFEEQLRIAKETGKPVIIHTRDAWEDTMKILRGSWTASGIMHCFSGGP
                     AEAKQALDMGFYLGFGGVITFPKADKVREAAKFTPLDRILVETDAPYLAPVPHRGKRN
                     EPVFVLDTARRLAELKEIPIEELAGATTGNFRRLCLPEAIGSE"
     gene            complement(18920..20848)
                     /gene="metG"
                     /locus_tag="VLE22_16105"
     CDS             complement(18920..20848)
                     /gene="metG"
                     /locus_tag="VLE22_16105"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSB15979.1"
                     /translation="MKPKYYLTTPIYYVNAAPHIGHAYTTIAADTIRRLKRMQGYDAV
                     LATGTDEHGQKVERAAKATGKNPQEFTDLISAEFRTQWETLGLAIDRFQRTSSPQHHK
                     VVQDLFRRCLENGFVYKGSYTGQYCVFDELYVSDAKPGDPCPDCGRPTETVTEENYFF
                     KLSAFQEPLMKLYEEQPDFIRPESRRNEVLSFVSKGLSDLSISRTTIKWGIPVPAEGN
                     HVFYVWFDALSTYMSAVEGEDRWPADLHLIGKEILRFHAVYWPAFLMAAGLPLPKRIF
                     AHGWLLFEQDKMSKSRGNIVRATPIKEVAGVDCLRYFLLREIVFGQDGNFSYDALVGR
                     YNSDLANGLGNLASRTLTMIRQYRGGAIPASGGSAEIAQLAGGTIRAAVEAFDNFDFS
                     KGLEAVWSLLAAADKYIVAQAPWTLAAKKDAESQAKLDETLYTAAEVLRISCALLAPV
                     IPGSMAKIWRQLGMGDDLEAVQLVGLAWGQLQEGQRIGEISAVFPRLDLKQSVEKMRA
                     LEREETIRQEILVGKRAAPAEPQASDKIPIDDFVKVDMRVGKVLTAEPVKGADKLLHM
                     SVDVGEAQPRSIVAGIAEAYRPEDLIGRKVVIVANLQPRKLRGIPSDGMIVAASVEGG
                     KPVLAGFHEEIPVGARLK"
     gene            complement(20845..22002)
                     /gene="larC"
                     /locus_tag="VLE22_16110"
     CDS             complement(20845..22002)
                     /gene="larC"
                     /locus_tag="VLE22_16110"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HSB15980.1"
                     /translation="MKICYLDAFSGISGDMLVGALADAGADAPTLVEALSSLAPGAEI
                     RFEKTVRCGIGSTKFHVSGGDNKRHRHLPHILGMIEQSALPDRVKRDSSTVFRRLGEV
                     EAAVHQVPIEKVHFHEVGAIDSIADIVGACLGIHMLGVETVYCSPVNLGSGTVDTDHG
                     TLPVPAPATAGLLAGKPVYARGPSVELTTPTGAAIAVTLSESFTPLPPMRISAIGYGA
                     GVRDFKEQANVLRVLIGEPTGAVEAATVSVIEANVDDVTPQVLGYTLERLLESGALDV
                     SFCPVFMKKNRPGTLIRVIARPEDRERLSQILFAETTTLGVRIFAAERRVQSRRMVEV
                     ETSYGKVRVKVGESGAYAPEYEDCRMLAREAGVPLKQVLAEANLAYLRTQQ"
     gene            complement(21999..22718)
                     /locus_tag="VLE22_16115"
     CDS             complement(21999..22718)
                     /locus_tag="VLE22_16115"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HSB15981.1"
                     /translation="MESLQQAREWLEAASAVVALTGAGISAESGIPTFRGNSGLWRQY
                     RPEDLATPEAFARDPKVLWEWYDWRRGLIAKAAPNAGHYSLAGLEKKKSSFVLVTQNV
                     DGLHDRAGSRNVLKLHGDIWHLRCTTCGVEHRDERTPLPELPPRCDCGGMLRPGVVWF
                     GEALPMNVWNDAERAARSADVCLVVGTSSVVYPAAGLTPLAKRAGARVIEVNLEETAF
                     SAQADCSLRGKAGEILPELIR"
     gene            complement(22756..23250)
                     /gene="purE"
                     /locus_tag="VLE22_16120"
     CDS             complement(22756..23250)
                     /gene="purE"
                     /locus_tag="VLE22_16120"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSB15982.1"
                     /translation="MTNDTKPLVAVIMGSKSDWETMRQTSETLDRFEVPHECRVLSAH
                     RTPTETAEYAAQADARGVEVIIAAAGGAAHLAGVVAAHTLVPVLGVPIESQSLKGLDS
                     LLAMVQMPGGIPVGTLAIGKPGAVNAALLAVAILANSRPGLREKLREFRQMQAKKVLA
                     EKLP"
     gene            23438..24301
                     /gene="cyoE"
                     /locus_tag="VLE22_16125"
     CDS             23438..24301
                     /gene="cyoE"
                     /locus_tag="VLE22_16125"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HSB15983.1"
                     /translation="MSNLMRDYFALTKPRITWLILMSTGVGYFFGLRGEWDLLTLLHT
                     ILGTGLLASGTATLNQWYERDEDAKMHRTERRPIPAGRVTPAKAAIFGILLSAAGFLE
                     LSLAVNLLTGLLGLITLASYLLLYTPLKKRSSLSTTVGAFPGAMPPLIGFAAASGTLT
                     AQAWVLYVILFLWQFPHFYSIAWMYREDYGRAGILMLPVVEPDGRSTSREIVLYSLAL
                     IPASLLPALLSMSGRIYLCGALVLSSFYFYTGLRVLLERTALRARKVLLASVLYLPLL
                     YGLMLLDRPGL"
     gene            24298..25209
                     /locus_tag="VLE22_16130"
     CDS             24298..25209
                     /locus_tag="VLE22_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB15984.1"
                     /translation="MIVSVENLTHNYAARTALAGVSFAVKDREVFGLLGPNGSGKSTL
                     FRILSTMMAPASGRVTVAGLDVGSRPAEVRRRIGVVFQSQSLDRKLTVEENLFGQGHL
                     HSLSGVTLRERVDEVTARLGLEDRRDEIVEKLSGGLKRRVEIAKALLHKPEVLLMDEP
                     STGLDPGARRELWQYIEDLRANQGVTVLLTTHILDEADRCDRLLLLHQGRIVAEGAPA
                     ELKSRIGGDVVVLEVADLDGMQRQLAERFHTEPRVVDGALRVEIANGHRFITEAVEAF
                     PGAISSVALHKPTLEDVFLDETGVAIC"
     gene            25230..25955
                     /locus_tag="VLE22_16135"
     CDS             25230..25955
                     /locus_tag="VLE22_16135"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB15985.1"
                     /translation="MATLWQRELVRFWREKARVLGFVGSPLIFWLVIGSGFGNLGFFF
                     PGALTLTVMFSAVFSTMSLIEDRREGFLLSMLVSPAPRSSMVLGKTLGSATLAWIQGM
                     IFLAFGPLAGVPLSFDVLWQSASVMFLIAMTFTLLGFALAWRMESSQGFHAIINLLLL
                     PLWMVSGSLFSMAKAHGWIRVVMVLNPMTYALAALRRVLGTQVSAGTPELGVSLTVTT
                     VCAVVFLTVSALVAGQKTARNFA"
     gene            25952..26530
                     /locus_tag="VLE22_16140"
     CDS             25952..26530
                     /locus_tag="VLE22_16140"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HSB15986.1"
                     /translation="MRSRATVPATILAGLGFLSLSGCAPKAEPLPVLGEIPEFTLTAQ
                     DGAEFHGGSLRGSVWVADFIFTTCAGPCPRMSWQMSRLQEAFSDKPDVKFVSFTVDPE
                     RDTPEVLAAYGKRYQAQAGRWYFLTGPEQALHKLNREAFKLGNVDGSLNHSTRFVLLD
                     RQSRVRGYYDSSDADSMKQLLADLKNLVRERS"
     gene            26527..26943
                     /locus_tag="VLE22_16145"
     CDS             26527..26943
                     /locus_tag="VLE22_16145"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HSB15987.1"
                     /translation="MIELRDLPAVNALLNLTAACLLVCGYVLIRRRRIQAHKRVMLAA
                     FSVSVLFLISYLVYHYQVGSVRFQRTGTIRTIYLGILFTHTVLAAVVPVLAVVTLFRA
                     LQERFDRHRRLARWTLPIWLYVSVTGVTVYLMLYRL"
     gene            27013..29103
                     /locus_tag="VLE22_16150"
     CDS             27013..29103
                     /locus_tag="VLE22_16150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSB15988.1"
                     /translation="MSTALRWSIAFASLGVVGAVAATTPKVIPARHKVRIERAVMIPM
                     RDGKRLSADLIRPDSEGRFPVVMMYQPYRKDDVGRGGVGPHQYFAERGFVAVRLDARG
                     TGTSEGVNTDEYTLQEQQDGYDAVEWLARQSWSNGNVGVWGISYSGFTALQVATHQPP
                     HLKAIVPIYATDDRYTDDCHYDRGGNMRMYYDVGHYGGWMVAMNALPPMPELAGPQWA
                     EMWTEHLQNNEPYLLKWMKQQTDGPYWRNGSLRPGYERIKCPTFLIAGWRDGYPNAMV
                     RTYMNLKVPRKLLVGPWVHSLPNSSVPGPRIDWMNEAGRFFAHWLRDEDTGIMTEPPV
                     TVYMQEYSVPDRMLDVTPGSWRNEADFPASGTSEITFYIDEGGKLASTVGAAPRRDHD
                     EFEYVPTVGLSNGYWSGGGIPFYLPDDQRGDEAYSLVYTTEPLDRELRILGWPKVILH
                     ASSSVRVATFVAKLADVAPDGHSALIVDGALNGTRRQSLTTPSAMSPGEVYELAIPML
                     PTGWVLKPGHRLRLAVSSSDFPNLWPTPEPARNRVFRGGRYSSRIVLPVVPESKLPAP
                     EFLPPPALRQIVSGSGSPPVMHALYDQIAGTVTVLGQRKGATILDEGLGTVLSDSEFR
                     CTASSRDPGRASIIGTHKIAFQREDGLIEVTGESSIRATTTHFHILVNLTVTRNGMPF
                     FQKQWVATEPRNLL"
     gene            29140..30510
                     /locus_tag="VLE22_16155"
     CDS             29140..30510
                     /locus_tag="VLE22_16155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB15989.1"
                     /translation="MKRLQPVLNRRQFVAGGVAASGMSCVAAGSSVPPNIVIILADDL
                     GYGDLSCYGHPTIQTPNIDRMEREGMRFTQFYSAASLCTPSRAGLMTGRLPIRSGLTR
                     VLFPWSEGGIQDSEITIARLLKKSGYAAMCVGKWHLGHHRRYLPMRHGFDGYFGIPYS
                     NDMSKATQPTASWVDRTPPTPLIRGEEVIEQEPDQRYLTRRYTEEAIRFIRESCRAGR
                     PFFLYLPHTFPHMPLFASERFRGKSLRGLYGDVVEELDWSVGEILAELGRLGVDGSTL
                     VAFTSDNGPATGSAGLLRDQKASTFEGGMRVPFIARWPGRIPAAVTTSAFGTTMDLFP
                     TCARLAGATLPTDRIYDGDDLTPVLLRNSTGREPLFFYYSNLDDDQELKAVRKGRWKL
                     HTSIGSFRKAPLLPNESGPPLLFDLNEDPSEQRNLAGTKPEIAKELRVVMEAHKASVR
                     PGPQQL"
     gene            complement(30528..30947)
                     /locus_tag="VLE22_16160"
     CDS             complement(30528..30947)
                     /locus_tag="VLE22_16160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HSB15990.1"
                     /translation="MKSYRKELWFDVPGRRAFLNITSQVEKCLAESGVQEGLVLVNAM
                     HITASVFINDDESGLHHDYDKWLENLAPHEPVSQYRHNDTGEDNADAHMKRQIMGREV
                     VVAVTKGRLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            31064..31552
                     /locus_tag="VLE22_16165"
     CDS             31064..31552
                     /locus_tag="VLE22_16165"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HSB15991.1"
                     /translation="MAIDHSLKTPKKARAGVPVAAIIILAMVVIGAAGFWYLDRQSKS
                     MPKDIPVLTAEAKAYVRNLKLGEVEMKAAEAFAGQTLVEILGKITNAGDRRLQSVEIN
                     CIFYDPYGQVVLRQRVPIVRSRSGGLAPGETKSFRLPFDEIPDSWNQAMPQLVIAEIN
                     FQ"
     gene            31561..31923
                     /locus_tag="VLE22_16170"
     CDS             31561..31923
                     /locus_tag="VLE22_16170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB15992.1"
                     /translation="MALILLVDDDPDQLEIRRLLLETSGFEVRTASTTGEAERLFGEC
                     PPALVVTDLRLPTPSDGIRLMRMMRARSAGVPILVLSGMTSDLAHVEDGLADEVLQKP
                     FRADRFLELVHEYAHGPR"
     gene            31910..32212
                     /locus_tag="VLE22_16175"
     CDS             31910..32212
                     /locus_tag="VLE22_16175"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HSB15993.1"
                     /translation="MDLAELRRSLELGREVRLKVKVVPKSSRSEIAGFMGDGTLKLKV
                     QAPPERGKANAQVCSVLAAAFGVPERNVSIVSGETSPLKQVVVAPPSDLRCRRAPG"
     gene            32239..33492
                     /gene="dgoD"
                     /locus_tag="VLE22_16180"
     CDS             32239..33492
                     /gene="dgoD"
                     /locus_tag="VLE22_16180"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HSB15994.1"
                     /translation="MPAACFQDSSTRRSVISSLFAAAGWSAFDRAEAAVRQSAGRDAP
                     ITGKDKLRITKLETFLVKPRWLFLKVHTNAGIVGLGEPITEGRALTCATAVKEIEPYL
                     VGKDPRQVVHHWQAIYRHAFYRGGPILTSALSGIDQALWDIKGKALGVPVYELLGGPT
                     RTRIRVYAHAGSPESIKEAMARGFTAFKTGPAKKRPARYVETPAAVQYAVEQFAELRQ
                     LVGKEADIGIDFHGAISPALAKLLIKGLEPYQPMFVEEPCQAQNHDVMAEIARGTHLP
                     IATGERVFTKWGFREVLEKGAASILQPDLCHAGGITECRLIAGMAEAYYASIAPHNPL
                     GPISLAAGVQLSASIPNFLCQEQVSLGEGYIKKPFTVREGYLDLPDGPGLGIELDENA
                     MADKIGHDWRNPETYDEDDGSVVDW"
     gene            33505..34170
                     /gene="eda"
                     /locus_tag="VLE22_16185"
     CDS             33505..34170
                     /gene="eda"
                     /locus_tag="VLE22_16185"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HSB15995.1"
                     /translation="MTKQTILSSIIDIGIVPVVRTSSAESAIKAIEAIYKGGIRAAEI
                     TMTVPGALRALEKVADKFGDKIVLGAGTVLDPETARACMLAGAEFFVTPALKPATIEM
                     ARRYSKVIMPGALTPTEVLTAWEAGADIVKVFPCGNVGGPKYIKALKAPFPQIEMVPT
                     GGVNLETAGDFLKAGACAVAVGGELVDAKTIKEGRYEVFEERARQYLAVVAKAREEMK
                     SAK"
     gene            34195..34365
                     /locus_tag="VLE22_16190"
     CDS             34195..34365
                     /locus_tag="VLE22_16190"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HSB15996.1"
                     /translation="MAISKDLLEILVCPLCKAPVELKQDENGLKCVECKRVYPIRDDI
                     PVMLVDEARIEE"
     gene            34379..35296
                     /locus_tag="VLE22_16195"
     CDS             34379..35296
                     /locus_tag="VLE22_16195"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSB15997.1"
                     /translation="MPDALQRLPGGARVAVVRLRSLGDCVLTTPALEILKQFRPDLRT
                     AVVVEDRFAPVFEGNPDVNAILPPAASALRGWKPDLCINLHGGMRSAVLTAASGARFR
                     AGFAHFRASRLYNLRIPTAQTILSVDRKVHTAEHLASAIFWLGVPRCEIPWARLFAHD
                     AGLSLRGSYAVLHPFATGPGKAWPASGFLAIASHLRLALGLDPVFIAGPGDDTHPFAE
                     YRCIEGAPLADVKSLLRGATLFVGNDSGPAHMAAAFGLPVLVIFGASDPVIWAPWRTV
                     SQVIVARGPIEAVAVGEVIEALERLRVAA"
     gene            35293..37116
                     /locus_tag="VLE22_16200"
     CDS             35293..37116
                     /locus_tag="VLE22_16200"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HSB15998.1"
                     /translation="MKEWLRLLGFVRPYVLVLVVSVVLMACVGGAHALIALLIGPVFD
                     RVLNPASADAPVLLFRLPIFGQAVYLNDLLPSWIHNVWTMIALGILAVFLVRGLCDYF
                     GNYLVNYVGYSAVTDLRQTVFDKILRQSAEFFESHSTGRLMSSIMNDIEKIQVATSHI
                     LADLMRQIFIVVGLLYVLLEKDWKLALVSLTLLPFVLVPTARIGKRIRRTTRRAQDDT
                     ADLNQILQETLSGHQVVRSFGAEAYESRRFRVVARRLRSSNLRYVAQQALSSPLIEFF
                     GALTIVGLLTYARTQIKAGQMTTGEFTTFVVALLMLYEPVKRLTGIHNIFQQALGASQ
                     RVFEYLDREEAIQDKAGALPLARFERSIVFDAVSFRYPSSPNGFLLRDISLEVKAGDV
                     VAVVGPSGAGKSTLVSLLPRFYDVTAGAVRVDGHDVRDLQVASLRRMVGIVPQDTFLF
                     NDTVANNIRYGRQDVSVREVEEAARNALCEEFIGQMPEGLDTVIGERGFKLSGGQRQR
                     IAIARALLENAPILILDEATSHLDTESELLVQRALGNLMSGRTVIVIAHRLSTIRRAD
                     KIIVLDQGRICEVGTHDELVGSGGIYQRLYELQFLEADRLA"
     gene            37119..38012
                     /locus_tag="VLE22_16205"
     CDS             37119..38012
                     /locus_tag="VLE22_16205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HSB15999.1"
                     /translation="MNVRSMTGFARVQKQNDVGEAAVSIKSVNHRALDLHFRMPGELD
                     PFEPVLRAAVKRHIVRGHIQIQVTFFRSSEAPPAALNRSVLDAYISAYRQAAAEHGLG
                     GEPDLNAALSLPGVFREAPDEEPDRTVEKLLLEAAEEALQALNAFREREGGEIVGELL
                     DRAAAIRDCTSRIEEIRSRALPAFQARLTERLAELLQNVPIDTQRLAQEAAILADRSD
                     IGEELTRLRIHAVQVEDLLRKGGEVGKKLDFLLQEMNRESNTILSKSNGIGEQGLEIT
                     QLALAARADIEKIREQSLNLE"
     gene            38009..38620
                     /gene="gmk"
                     /locus_tag="VLE22_16210"
     CDS             38009..38620
                     /gene="gmk"
                     /locus_tag="VLE22_16210"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HSB16000.1"
                     /translation="MSIVFIVSAPSGSGKSTLVGRLLKDVPRLLFSVSYTTRAPRGNE
                     RQDEDYHFISRAEFEERIARDEFLEWAEVFGNYYGTHREILDRARSEGSDVVLDIDVQ
                     GARQLKRRILDAVSIFILAPSREILEQRLHDRSEDPPEVIARRLREAAEEIRNCRAYD
                     YVLVNDEVERSADTLEAIVKAERARRTRIEEKIRPILATFEAK"
     gene            38641..38886
                     /gene="rpoZ"
                     /locus_tag="VLE22_16215"
     CDS             38641..38886
                     /gene="rpoZ"
                     /locus_tag="VLE22_16215"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HSB16001.1"
                     /translation="MSDRLSRNASFIIPDDPEQSTYRWIIVAAKRARQLQGGARPFLP
                     TTAKKPTVTAMEETRRGLVKWEDLALQPQEEQEQEQV"
     gene            38891..40102
                     /gene="coaBC"
                     /locus_tag="VLE22_16220"
     CDS             38891..40102
                     /gene="coaBC"
                     /locus_tag="VLE22_16220"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HSB16002.1"
                     /translation="MKVVLGVGGGIAAYKAAELVRALSERDAEVQVVMTEAAQEFVRP
                     LTFVALSGRKVITSMFSSGGAEETLSSAVEHIQVAQENDILVVAPATADLLARFAHGI
                     ANDFLTTFYLAFTGPVVLAPAMNTNMWNHPATQANIGVLRSRGHSIVGPEDGFLACGM
                     VGPGRMSEPAEIAEAVLSLSRRRDLEGETILVTAGPTQEPLDPVRFVSNRSSGKMGYA
                     LAQAAADRGARVVLISGPVHIPEPSGVQIVHVRTATEMRDAVLAGLDESTIIVKSAAV
                     ADYHVSTVPQQKMKKTAARISLELDPTPDILAELGQKKGDRLLIGFAAETENLRQEVR
                     RKLASKNCDMLVGNLVGQEGVGFESDTNEVLLGLATGALISIPKASKREVADRIFDEA
                     LKLRLALHAAQ"
     gene            40099..40854
                     /locus_tag="VLE22_16225"
     CDS             40099..40854
                     /locus_tag="VLE22_16225"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSB16003.1"
                     /translation="MTRELLRQYLSFYQDLGIRTLYRREPHAAPPSASREPAPLDLLL
                     QPQAEPEAVQHFSEPPTGDTLARIREDIGDCTRCRLHQGRNKIVFGSGNEQAKLVFVG
                     EGPGADEDAQGLPFVGRAGQLLTQMIEGTARKEGIPLVRKDVYICNVVKCRPPENRTP
                     LPDEMETCGQFLFRQLSVIRPKAICVLGATAAKALLDRKEGVTRMRGQWFRWHDIPVM
                     VTYHPSYLLRPYNQNAKREAWEDLKKVLHYVYD"
     gene            40847..41518
                     /gene="tmk"
                     /locus_tag="VLE22_16230"
     CDS             40847..41518
                     /gene="tmk"
                     /locus_tag="VLE22_16230"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSB16004.1"
                     /translation="MTERRRGFFITFEGIDGCGKSTQMCLLAERLRREGHRVFETVEP
                     GGTSIGRQVRRILLDAKNQELCPTAELLLYFACRAQNVEEWILPALERGEIVVSDRFT
                     DSTMAYQGVGRGLGEDVVRDLHRIACRDLTPDLTLVIDIDLETSLERARTRNRELADT
                     GAAPETRMDDQAVEFHRRVRGAYAAMVQVEPERFVVIDGRADVESVARAVWEAVAPRV
                     GGLHV"
     gene            41511..42539
                     /locus_tag="VLE22_16235"
     CDS             41511..42539
                     /locus_tag="VLE22_16235"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HSB16005.1"
                     /translation="MFDDFHGNERVAAALEKMAAQDRIPQTLLMVGPQGIGKATLARR
                     FAARLLGGADKIERDDLSLPHNIELRGEREKWPSEKRAEEPLLLASQPDFVTFPPDGP
                     LRQISIQQMRLLKERAQFLPLHGRWRIFLIDQIDRANEQAANSLLKTLEEPPDHLILF
                     LTAENAYDLLPTIRSRAVPLYLARLSDSEIRRFVAGRGVSDVERRVSLAGGSPGLALS
                     IDLEEYDARREAMVALLEVASGVSPFSAWLTYSDKISMSRQEKLEDYLKVLYGLLADL
                     LAVQQSGGGIRNVDLRARLEGLASRVNFDWIRAATGKVDELVRLLRRNIQKSIALDAL
                     ILDLRPVV"
     gene            42697..43005
                     /locus_tag="VLE22_16240"
     CDS             42697..43005
                     /locus_tag="VLE22_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16006.1"
                     /translation="METANRRLIRPSLNNAKEQAASRRNQTQKKTIPPDQTHAESFYY
                     LKQMQSKTPMVVVLKDGETVHGIIEWYDRCCLKVNREDGPNLLIYKPSIKYMYKATEA
                     "
     gene            complement(43018..43494)
                     /locus_tag="VLE22_16245"
     CDS             complement(43018..43494)
                     /locus_tag="VLE22_16245"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HSB16007.1"
                     /translation="MQARPVRESISEYSEFALPNDTNALGTMLGGRVMHLVDLAGGLA
                     AMRHARRPVVTASIDHMNFLHPVHVGQLVVLRSSVNRVFRTSMEVGVTVMVEDMTTGE
                     RKHTSSAYLTFVALGGKGEPVEIPPVIPETRDEKRRYEEAGQRREYRLQLRRRAKN"
     gene            43527..43841
                     /locus_tag="VLE22_16250"
     CDS             43527..43841
                     /locus_tag="VLE22_16250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB16008.1"
                     /translation="MPEDRIEILKRMVAASPKDSFARYGLAMEYVKAGDFEEAVREFR
                     ELVVFDPGYCYAWFHCGQALEKLGRANEAREIYRQGIEAAERKGDTHARSELQGALDI
                     LG"
     gene            complement(43865..44338)
                     /locus_tag="VLE22_16255"
     CDS             complement(43865..44338)
                     /locus_tag="VLE22_16255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSB16009.1"
                     /translation="MASRSVNKVILVGNLGKDAETRFTPSGAARTTFSIATSRRWKDQ
                     QTGEWKEETDWHNVVVWRQENLANYLTKGKQIYVEGRLQTRSYEDKDGRKVYTTEVVA
                     EDVILLGGRGEASPAADEHSQVVSMPRSSARSQPAPAQQEEPASGYSATDDDVPF"
     gene            44600..45832
                     /locus_tag="VLE22_16260"
     CDS             44600..45832
                     /locus_tag="VLE22_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16010.1"
                     /translation="MRFVSALFLGITLVTLCVRPSSVRGQVPITTSGPYGVVRLFGSD
                     AAVLEVEEPRRDLPCTLTPGKALLGFDLRMHAGYEVAVPLRELSGAENILTIVFRVTP
                     AGRDEDPVYFSQRVRVPPIEEDAKGDAYLQGAFDLGEGKYHVAWLMRDRAERVCSSYW
                     DIDASLASKDKEIGLILPPGTVQASEFEPFKDEPPVERTQGEPPLTVKVLVNFAPQNM
                     HSAVLQPIDTSALVSILRSIVREPRIAKFSLVAFNLQEQRIVYRQEDADRIDFPALGD
                     AVESLNLGTVDLQRLSHKDGETQFLTDLIRRETGGDRPPDALIFAGPKAMLDTKVADD
                     SLAELGPLDYPVFYMNYNLNPQFNPWRDAIGHAVKFFKGTEFTISRPRDLWYAVTDMV
                     SRIVKSKSERRTTAASAR"
     gene            45836..46399
                     /locus_tag="VLE22_16265"
     CDS             45836..46399
                     /locus_tag="VLE22_16265"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB16011.1"
                     /translation="MRTVGILPIAVQSLLLFAATLSAQQRVHPDNLAPFVPTPEPVVE
                     KMLEAAELKSDETLYDLGCGDGRILFVAAQKFMAKAVGVELSARLVKSTTEKAEKLGL
                     ADRVSVIEGNLLDVDISPADVVSIYLLRLSNERLKPNLKKQLKPGTRVVSHDYEIMGW
                     KPYRTEKVSVHQRTHTIYVYKMPPVEQ"
     gene            46444..47394
                     /locus_tag="VLE22_16270"
     CDS             46444..47394
                     /locus_tag="VLE22_16270"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSB16012.1"
                     /translation="MTSAFDVAGVGLNATDTLLIVPRFPPYAGKILFEEEILSPGGQV
                     ASAMVACARLGLRVKYIGTVGDDERGRIQMASLKASGIDIEDVKVRPGCPNQSAYILV
                     DRSTGERTVLWRRPDCLRLDPSEILPEQILACRMLHIDGHDTPAVEKAARIAHSASIP
                     VTVDVDTLYHGFDRVLPLVDYLIASSGFPVQWTGEDDPFKALEVLQNEYGMRVAAMTL
                     GAHGALARMERRFVYSPAYVVNCIDTTGAGDVFHGAFCYAVLKSMPIEEALDFSNALA
                     ALNCTALGARGGIRGVEEARALMLRAERRFHPDFASHTGR"
     gene            47398..48066
                     /locus_tag="VLE22_16275"
     CDS             47398..48066
                     /locus_tag="VLE22_16275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HSB16013.1"
                     /translation="MIPLRDSQRSNSTPVITVGLIVVNVLAFLYQTSLDKFSLNHVIM
                     EYGVVPSRLQLTSLVTSMFLHGGWMHLIGNMWFLWIYGDNVEDILGHAKYLIFYLACG
                     VAAALAHVALNGDSRVPTIGASGAIAGVMGAYMVKFPRARIITLIFLFVFVTTIEMPA
                     YAILLYWFVIQIFSGVGSIGYSQISQGGVAWFAHVGGFVTGIVLVMLMRTREPYRLHR
                     ELHW"
     gene            48066..48791
                     /gene="bshB1"
                     /locus_tag="VLE22_16280"
     CDS             48066..48791
                     /gene="bshB1"
                     /locus_tag="VLE22_16280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HSB16014.1"
                     /translation="MDTPAHPIDILAIAAHPDDVEQTCGGTLIRMAEAGYRTGVLDLT
                     AGDMGTRGTPELRIQESGEAAKHMLLSWRGNVRMPDARLENTLAARMTLAVKIRELRP
                     RAVILPYWQGRHPDHYRASEIGYEACFLAGLRKIDEYSEPHRPHKILYASMYANVTPS
                     FVVDISAQFERRMSALLSYRSQYGEISDGAALFPNESEIRERHAAIARFYGNLIGVRY
                     GEPFVVKETMRVDDVVALGPRSL"
     gene            complement(48805..50082)
                     /locus_tag="VLE22_16285"
     CDS             complement(48805..50082)
                     /locus_tag="VLE22_16285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB16015.1"
                     /translation="MSASQLAQKATRARYWVIVFAITLSILAYIDRVCISMAAPNMMR
                     DLGLSKVQMGYVFSAFALAYALFEIPGGWMGDAWGPKRVLVRIVLWWSFFTAATGWVW
                     NMASLWGVRFLFGAGEAGCFPNLTKAFTTWLPQHERVRAQGIMWTFARWGGAFTPPLV
                     ILVFDYMSWRQAFGLFGLLGLLWCAFFLRWFKDDPRDHKSVNGAELALLAGSEKMASG
                     HGDVPWKKLVSSRTVWLLWAQYFLLSYPWYFYITWLPTYLQESRGLDPRQASRLAIFP
                     LLFGGFGSLFCGLVSAGVARRLGSTRTARRLLACIGFAGAGLLIILSINLKDPLLAMV
                     AMGMASFANDLVMPGAWGACMDVGGKYAGTLSGSMNMMGNLAGFAAPTVTGYVLRWTD
                     GNWNVNLYIMAGVYLLGTLCWPFIDPVTPLDKD"
     gene            complement(50151..50477)
                     /gene="cutA"
                     /locus_tag="VLE22_16290"
     CDS             complement(50151..50477)
                     /gene="cutA"
                     /locus_tag="VLE22_16290"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HSB16016.1"
                     /translation="MTDKIVVLSTCSTAEEAARIGRQLVEKRLAACVNVVPGARSIYR
                     WKDEIEDAPESLLIIKSRRGLFDALRAEIEKLHSYEVPEVAAVAIVEGSPGYLTWLGK
                     ELGARE"
     gene            50544..50861
                     /locus_tag="VLE22_16295"
     CDS             50544..50861
                     /locus_tag="VLE22_16295"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HSB16017.1"
                     /translation="MNDLPNSRVRKIVRAAWPLVALLGVGVYVYATLRSPQGLSAVLE
                     KQRQIRDLQQQNANLDRDIQQKRERIRKLKEDRNEQEMVIRERYHLLKEGETSFILQD
                     QKK"
     gene            50952..52049
                     /locus_tag="VLE22_16300"
     CDS             50952..52049
                     /locus_tag="VLE22_16300"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HSB16018.1"
                     /translation="MRSTKLSRRDFAGIALCGTVAASTAASASKLEPNPPGVKISIQS
                     GSDPTDDDLNFVRQLGIGYINLGTHGEQATAENFLRLRNRAESFGLKVWNIGNTDVHN
                     MEEVTLNLPGRDRKIEQYKNYLRNLAKAGILYTTYAHMGNGIWSTAPEKIRGGATARA
                     FDLAKAEQGVWAGKIFKGPLTHGRKYTKEEIWDNYTYFIKQVTPLAEELGIRIGIHPD
                     DPPVPELGGVPRCIFSSFDGYKRALEIANSPNVGICLCVGCWLEGGKLMGKDPVETIR
                     YFGRQKKLFKVHFRNVTAPLPHFVETFIDEGYFDMYKVMNALVEVRFDGNVIPDHIPA
                     MVGGRHAATAHSIAYMKSMLERATTEACGRA"
     gene            52046..54391
                     /locus_tag="VLE22_16305"
     CDS             52046..54391
                     /locus_tag="VLE22_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16019.1"
                     /translation="MTSAGRIAIQAFGLYLTVCLLASGAELAADHPRLVFQPGDRRGH
                     GRTFEEIRKLHRDDGTFRSIIEKALALDLEKQHPAMPAASWIVSGEDRYAEAAIERML
                     SAPISRSGEGSYSNIWSHALAYDWLYHHPRFTPEKKERAATKIVERLATELAGLDDSG
                     MALWHGRNQAANGAMVAALAVGDLPGQRENLTRATAHYIESLRALRFSEGWPEGASYW
                     IYNRAGPYALAADCVMTALGVDSIGGVPIREVMRKIGLWSLYQFAPNGVFEPYGDSGG
                     SLQLGETGWWELTTDYFARLSKDPSLMAGADYLRNRSPNPYGRRPYNWWAVLSYDPTA
                     RPRRGYDPAKPEIWMRARLPQSMLFGRDSIGVAFFRGEWGDPDELYATFKAGDMLAHH
                     DHYDVGHFGIQFGGLLAPQTGIYGSGTDGYDGAYRLGYGVQSVSVNSLLILAPGEISA
                     DLRSRKNPAWTSLSGGQRVISPTGFSCVSLDDFRHKLQTGRHLERGGITVFESIAGEL
                     DYIAADITASYNSTKWAEPGTEPKVSLVTRQFLYLRRERAFVVYDRVETTNARFLPKF
                     LLHSLSKPVTQSEKLLAGSGPEDGILETTDRTLRTADQRGELTQLVLLPRDARALKIG
                     GPNYYGYVESDGDQADGFDGVNLSAGDPRIPRKTAQLGLWRTEVEPVLSGTSTRFLNV
                     LLPRLVARRGSLPVVDLAGSGEGADAVRVGNTVIVFARRPGGLDAVELDLSQPAEVIL
                     VDAVPGVRYRAGGRTATASGEGVLRVPGLARGRQTVARTGR"
     gene            54454..54795
                     /locus_tag="VLE22_16310"
     CDS             54454..54795
                     /locus_tag="VLE22_16310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSB16020.1"
                     /translation="MALAIAVRQVGSVAVMDVSGRLVLGEECSIVREKIRELLASGQK
                     NILLNLSEVTYADSAGLGELVASHVTVAKAGGRLKILNSQKKLDGLLEITRLHTIFEV
                     FADQDLAVLSF"
     gene            complement(54812..55504)
                     /locus_tag="VLE22_16315"
     CDS             complement(54812..55504)
                     /locus_tag="VLE22_16315"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HSB16021.1"
                     /translation="MKLTFLLFLFVAGVALAEGPGVIKSYYSSKDFALTADPNAAQWK
                     NVAGVFAENGPTKEPSPGHRTEIRSRWTDKNLYFLYICPYDKLFLKPNPSTTTETNKL
                     WDWDVAEAFIGTDFKHIRQYKEFQVSPQGEWVDLDIDRDNPKPEGGWLWNSGFKVKAR
                     IDEKKKIWYGEMQIPMASIDSRKPAPGLEMRANLYRIQGPPPKRVHVAWQPTGQMNYH
                     VPEAFGRLRLEK"
     gene            55535..56389
                     /locus_tag="VLE22_16320"
     CDS             55535..56389
                     /locus_tag="VLE22_16320"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB16022.1"
                     /translation="MRNLVLTLFLLAALCPAQSFEPVTTLPDWVSVEANVPYDQYKET
                     VLDIMQPKAAATGKRPGMLVIHGGGWVGGTKEQVVESFCLPYVEKGFVVANVEYRLAK
                     VAPAPAAVMDVLKAADWFRRNAGRYNVDGKRIAVIGNSAGGHLALMVGMASKSAKLGP
                     TGKVAAVINIYGISDVADQLGGPNTRDYAVTWVPDEPGRLELARRVSPMTYVRRDLPP
                     ILTVHGDADETVPYEHGTNLTKALRQAGATAEMISVPQGKHGFSKEQWAGVLPQIFKF
                     LERHRILR"
     gene            complement(56416..57765)
                     /locus_tag="VLE22_16325"
     CDS             complement(56416..57765)
                     /locus_tag="VLE22_16325"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB16023.1"
                     /translation="MLNRREFLAAGAGVAASSGRERKPNVIVILLDDMGSHDLGCQGA
                     TDVKTPNIDSLAAGGARFANWYSNAPMCAPARATLMTGRYPLRCGMPRNGMSLPVREQ
                     TIPALLKPAGYTTGLVGKWHLGSTPETAPNSRGFDYFYGFHSGCVDFYSHRYYWGEPR
                     VVNYHDLWRNRTEIFEDGQYLTERLTQEAVGFIQANHSQPFFLYLAYNAVHYPMHAPR
                     KYVERFPELEPERRMYAAMLAAADDGVGEVLRTLDRGGIRDNTLVIFSADNGATTEAR
                     AGLSQQAATAGRNSPYRGFKFSLFDGGMHVPAVFHWPGVIQPGQVLRETAMTADLLPT
                     ICTAAGVPLPAGRTIDGRDALPLMTAGAKSQHPDVFWDSDKQQAVRRGRWKLVVNGAV
                     YDGTPEGRNPLRGDDAMFLSDLEEDPGETRNLRRKHPELVDELATAISRWQEAVTKD"
     gene            complement(57773..58894)
                     /locus_tag="VLE22_16330"
     CDS             complement(57773..58894)
                     /locus_tag="VLE22_16330"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSB16024.1"
                     /translation="MNIPRWPLATDREMELLKEVIDSLQWGGFHEMVERFERDFAAYQ
                     HCRHGVSCANGTVAIEMALEAAGIGPGDEVIVPAISFISTATAVSRVGATPVFVDIEP
                     YSFNMDPDCAAAAIGPRTKAIVPVHFGGPLADMDRLMKLAADRGLMLFEDAAHAHGSE
                     WTGRRAGSLGVAGTFSFQNSKVMTAGEGGILTANDDGIAARARAIANQGRREGEGWFH
                     HFTLGTNYRITALQAAVLIAQLERLPEQIRLRMENACRLRAATSDVDGLTWQAVPSQV
                     NVNSYYLLLGRIDAAKFGMTRDEFHAALTEREIPCTPFYPHVLYQNPLYQKGACRVEP
                     CPVSEACIHDAFWLPHRVLMGDEETTMEIASVVLKLQRR"
     gene            complement(58916..59548)
                     /locus_tag="VLE22_16335"
     CDS             complement(58916..59548)
                     /locus_tag="VLE22_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB16025.1"
                     /translation="MIHEVLPVGMLQCNCSVFGDEQTREAIVIDPGDNVEEILGILER
                     HRLRATLIVITHAHIDHVGGAAKLKAVTGASVLMNEDDEGVYDQLDMQASWLGMATPR
                     RVEIDGKTRDGDTVRLGSSDFSVLHTPGHTPGSICLWLPSENKVIAGDTLFRDSIGRT
                     DLPGGDFRKILVSIRDKLLTLPEETLVLPGHGDTTTIGREKEFNYFLQGL"
     gene            59624..60931
                     /locus_tag="VLE22_16340"
     CDS             59624..60931
                     /locus_tag="VLE22_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16026.1"
                     /translation="MRGVAEALSALRTMLEAETPVGDSARSFLDLTVRLRDSVQPVLG
                     GTCTTACDRLAERARVLERTGDLEAHLLAGVQLESTLACIGEAIADNTEAKAQHVQQA
                     LARLLQRACVLAAGTSSPDWNSFPDLISTLAVVRRSAGELAGLVSTCGGDLARNAAEL
                     NQLLESSEARLLEDPCAMVASLTSELRWHLGLAVRRDWCFLHEDMDDSSLAAYTNTWR
                     EMLLPADAQITPEGILDAVSLSQQQFRICEIPDLDDYLDEPEHLIGYGLANLIPPAPY
                     GWEFSSGTAVQAFLRGQLGQWYMRPFRDGVSPVEMISTVLKFGRPLYYERILLHSVAQ
                     HRLVQHAVWPDGGGVSQLETLAHLEDFFNVMLDGYLLRKISMPNLRRPGGWIEYLEDL
                     IALHFAVTPQKVVEARKDFLARRGFDSGAQLLESGGHGGVFLN"
     gene            60977..61918
                     /locus_tag="VLE22_16345"
     CDS             60977..61918
                     /locus_tag="VLE22_16345"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HSB16027.1"
                     /translation="MSGMRMEGYRALREGAAWLDLTGRGKIAVRGEDRARLLHALCTN
                     HIQELTPGRGCYAFFLNAQGRILGDANVFCLSGHFLLDTEPELASKLYDHIDRYIIAD
                     DVSLEDMTGGLATIGLEGPKAAVVLSAAGATIPETPYAHAPWGDRMIARANTTGSGGF
                     LLFVPAAEKDATIRALEAAGAVAAGPDDTRTVRVENAVPRYGEDITEASLPQETRRDD
                     AINFNKGCYLGQEIVERIRSRGHVNRSVVRLEIEGDHPLPAGARILAGGNETGQITSA
                     EFSPALGNTVALAYVHAVDARPGTRLTVDGAVAVVSV"
     gene            complement(61960..62511)
                     /gene="cysC"
                     /locus_tag="VLE22_16350"
     CDS             complement(61960..62511)
                     /gene="cysC"
                     /locus_tag="VLE22_16350"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HSB16028.1"
                     /translation="MHKGFTLWFTGLSGAGKSTISEILAGRFREAGMKVEVLDGDVVR
                     THLSKGLGFSKEDRDINIRRIGFVCDLLSRNGVIAIAAAISPYRAIRDEVRAQTANFV
                     EVYVHCPLDVLVERDVKGLYKKALAGEIAQFTGVSDPYEAPLNPEVTVDSSRETPEES
                     SDKVWSKLEQLGLIPNGIPAMTR"
     gene            complement(62504..63250)
                     /locus_tag="VLE22_16355"
     CDS             complement(62504..63250)
                     /locus_tag="VLE22_16355"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HSB16029.1"
                     /translation="MTDISQLERLPAPDLLRWAIGTYGESFAICTSFQKTGMVILDMA
                     ARISTEVRVVTLDTGRLPEETHQMIDTVREKYGVRVETASPDPAELEAMVRSYGVNLF
                     RQEPALRQLCCEIRKVRPLERKLAGFRAWATGLRRSQSVTRASVPKAEEENGRLKLNP
                     LADWTGGDVEDYIRRHDVPVHPLYAQGYASIGCAPCTRPVAPGEDERAGRWWWEQDEH
                     KECGIHFGNGGAVLRGPAVLVNDILQGTNA"
     gene            complement(63341..63961)
                     /locus_tag="VLE22_16360"
     CDS             complement(63341..63961)
                     /locus_tag="VLE22_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16030.1"
                     /translation="MLLRAVLLLIAVAGIVAAQVIEFESGGLKYQTLSKNGLTVMFAH
                     LPIQVREYSILQVAASNGATIPCMVRPEDFVFTRDDGFAVRASSAESVVGELLNRAGR
                     NDVVKLTSTYEMGLYGMVRFRSTNGYEQRRRSALAEVASTKLKAAAAASAIAFVETRL
                     KPGESTDGAVFFPTLGRPLGAGKLRFTAGGQLFEFDVDPISDGPVR"
     gene            64155..64448
                     /locus_tag="VLE22_16365"
     CDS             64155..64448
                     /locus_tag="VLE22_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HSB16031.1"
                     /translation="MEALGMIETKGLVGAIEAADAMVKTANVILTGKEYVGAGYVTVT
                     VRGDVGAVKAATDAGAAAARRVGELVSVHVIPNPHEEVEKVLPGAEKGKKSHG"
     gene            64497..66320
                     /locus_tag="VLE22_16370"
     CDS             64497..66320
                     /locus_tag="VLE22_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSB16032.1"
                     /translation="MPHEQDPAAIEEVRSKVEKAYAAWLKYRNFTQEQIDVIVERAAA
                     AGRAHARRLAELAVEETGYGNVDDKIAKNLLCAEFLPKAMRKVKTVGLLREIPGQKVV
                     EYGVPVGVVAAIVPTTNPTSTAIFKILISLKAGNGIVISPHPRALRSTCETAEVLYRA
                     AVEAGAPEDIVQCLGKPTLEGTQALMKHERTAVILATGGSGMVRAAYSSGKPAFGVGP
                     GNVAVLVDPSADIPDAVERIVAGKSFDYGTVCSSEQALVAEESLRDRIVAELKARKAY
                     FCTEEQKETLGKLLITPNWTISPKFVGQSPTKIAEMAGFTVPADTSIIVAELQGVGKQ
                     HPLSVEKLSPVLALYFVKDHGAALDTCEAVLRFGGLGHTCVIYAKDESRIREFAQRMP
                     AMRVLVNTSAPQGSVGLTTNVFPSMTLGCGAAAGNITSDNVGPQHLINVKRLAYSVRE
                     PDEALVLPPKVAETQPVSMDRQSIAAAVEQYLAARGIRLEGERPAAATPVPAAAVVPQ
                     VSDIVDRFLAARRAAGPAIAPAKEASCGCPVHPPAPPAPGAAPPAPPAPEVQIVDFVC
                     ENDVRAAMRDSRKIFIGPKTILTPSARDLGERENVLVVAQR"
     gene            66321..66812
                     /locus_tag="VLE22_16375"
     CDS             66321..66812
                     /locus_tag="VLE22_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16033.1"
                     /translation="MKQNLDTLKSEILEQLGAQGFAVFHSYSRNLEDTGTIYWDVHHY
                     PDFRPFLDTARQLGVKMVTFHHREFTREAVEDAFDRLEECEISPEERRSYERRLREMG
                     GYEGFTCAVELAYDFETQLYLYSLQTAWYTEFLDLLDEIDGYTSDEEAEDEGPIGGYF
                     SKN"
     gene            66823..68565
                     /gene="recJ"
                     /locus_tag="VLE22_16380"
     CDS             66823..68565
                     /gene="recJ"
                     /locus_tag="VLE22_16380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HSB16034.1"
                     /translation="MQPQVRWSLPQADPEIVESLAAALGVQFPVARVLWRRGYREPEV
                     ARRFLDPSLADLHDPYLLRDMRPAVERLRRAILEKEEILLYGDYDVDGAMSVVILKKA
                     IELAGGAARFHVPHRLRDGYGMRPDVVEKAASAGVKLIVSVDTGIRAAGVVVHANELG
                     IDVIVTDHHLPESELPPACAVVNPNRTDCAYPEKNLCGAGVAFKLVQALFGTLGWTAA
                     RQRRMLESFLKLVAIATVADVVPLTGENRILVKHGLAGLRTVRNAGLRALLDVAGFAD
                     GDGPSASQVAFRIAPRMNAAGRMADANDVIDLFLTQDTKRARELADQLHALNQERQQA
                     EAEIVQTILEECERTPVTGDQMALVFSGENWHRGVVGIVANRLVEKFNRPAFVLSEDP
                     EEGLAHGSGRSVRAFHLLEALESMSGLFTRFGGHRQAAGLTVPVDRISEFRRRLNEYA
                     AARLTADDLVQELDVDAVLDLREISEKSVNEILRLAPFGFGNPAPLFAVLDAELAGPP
                     VVWKDKHLRVGLKRNGRTVIAKAWNFVDRAEELVAGARLDAVISLQADPYSLSRGYPG
                     WSAEIKDVRRSQER"
     gene            68634..69425
                     /locus_tag="VLE22_16385"
     CDS             68634..69425
                     /locus_tag="VLE22_16385"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB16035.1"
                     /translation="MIRTGSLILALIILAPPAEPQGIELNDGEDHGDPPFLLEEGWRP
                     LFNGPVLQGWHGQDGKPHEWMTTRGVRMDRYGPTRLSPVPTPGDRLINGPQGRTVNLV
                     TDEKFGDVELYLEFMIPKGSNSGVYLQGLYEVQVFDSWGSLEPVKSSDCGGIYHRWID
                     NKGVGGSAPSRNASRRPGEWQSFQIWFRAPRFDGSGKKVENAKFIRVLHNGLSIQENV
                     EVDGPTRAHMSHPEAPLNPLMLQGDHGPVAYRNIYIRSLRPIIER"
     gene            69432..70073
                     /locus_tag="VLE22_16390"
     CDS             69432..70073
                     /locus_tag="VLE22_16390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSB16036.1"
                     /translation="MPAGRGWIDISVPLRTGMVHWPDDPPVRIERFQSFENGDGCNVS
                     ALSMSAHTGTHVDAPFHFAAEGEGVDVLPFSATIGPARVVAIRSPEAVRPEELRAHRI
                     RRGERVLFKTPGSARRLKSGAFDEDFVYISKEAALFLAERQVRCVGIDYLSVGGFRKD
                     LAETHEILLGAGIWVIEGLDLSRVKPGRYELICLPLRIAGADGAPARAVLRPA"
     gene            complement(70085..71002)
                     /locus_tag="VLE22_16395"
     CDS             complement(70085..71002)
                     /locus_tag="VLE22_16395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB16037.1"
                     /translation="MDRRTFLELSSLSLGSRLKVLAEAPMPMATLGKTGLHVSRFCLG
                     GYHMGMHGEETGVRMIRRAVDLGVTVFDSAHHYNDGRSDEIYGKALAGGLRSKVLLMS
                     KAQLRDRDSAMRQLDETLKRMRTDYLDLWQCHEVSTQAEVDQILGPNGALEAFVKAKQ
                     QGKTRHIGFTGHRDPAVHQRLLDAFDGWETVQHPVNLIDPHYRSFIQSVLPNIRKKGL
                     GLLGMKSNGMGTITKNGIASIRECLRFALSQDIDTLVSGPETPEQLEENVLVVKTFQK
                     MTSDEMSALLARTKKGPYGPKIEQYKRES"
     gene            complement(71037..71435)
                     /locus_tag="VLE22_16400"
     CDS             complement(71037..71435)
                     /locus_tag="VLE22_16400"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSB16038.1"
                     /translation="MATFGLIEYDQASPEVRAVYDDIMATRNTDWINNFWKAIAHDPA
                     TLKRTWESIKEIMRPGALDPLTKEMIYVAISATNGCGYCIASHTASARKAGMTQEMFA
                     ELMAVVGMANETNRLANGYQVETDERFRTS"
     gene            complement(71466..74033)
                     /gene="polA"
                     /locus_tag="VLE22_16405"
     CDS             complement(71466..74033)
                     /gene="polA"
                     /locus_tag="VLE22_16405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HSB16039.1"
                     /translation="MSRPRLFLIDAFGFIFRAYHARARSGAPPMRTRSGLSTEAVYIF
                     HNMLRKLISAHHPDYIAAIFESGPSFREQEFADYKANRAETPPDLLEQIPYVRRLLET
                     MRIPVLEHAGFEADDVIGAIAHQAKAAGVDVVIVSSDKDMMQLVDDRVSMLNPTKDDT
                     LYDPAKVEEFMGVAPSQVADLLALKGDAVDNIPGAPGIGEKGAKDLIARFGSVENLLE
                     RASEVERKMYRESLLNHRDQVLLSKRLATIDTTVPVEWDLGTFQARPPDDSALREIYK
                     DLEFFSLLKEVGGGDSEPRDYRAIETGEELAEYLAAIPPEKPVAVAVQAAGEGELSLS
                     SLGLAGKCGEGRAVPFSLLEGVRGVLEDEHRPKVAHDAKTVLIELARNGIEARGFTED
                     VMLYAFLLEADPSGCSLEQLAQRRFDRKLGGSPEERADCALELAGVLGAEVDAQCLRN
                     VYDTIDMPLVSVLTRMERTGIRIDPSALGELSNRMDSEIQRLTAEIHSLAGRPFNINS
                     PQQLGKVLFEDLSLAVPVRYGKGKAPSTAADVLEELAAEHLIAAKVLEYRQLAKLKGT
                     YVDALPALIRPDTGRLHTSFNQVGAATGRLSSSNPNLQNIPIRTELGREIRAAFVPRE
                     GWLLVAADYSQIELRLLAHMSRDPVLVEAFRNGEDIHTRTAAEVFGVPPLMVTPDLRR
                     NAKAVNFGIVYGLSAFGLSGQLGIPQSEAQSYIKSYFERYAGVRKFIDDTIAEVRKNG
                     VTRTLFGRRRPIPDIASRNPNARGFAERTAVNTPLQGTAADLIKLAMIRIDRELRSRG
                     LQTAMLLQVHDELLFETPPSEIDEVSKLVKHEMESVHRLEVPLVVDIGAGSNWRDAK"
     gene            74219..75109
                     /gene="lpxC"
                     /locus_tag="VLE22_16410"
     CDS             74219..75109
                     /gene="lpxC"
                     /locus_tag="VLE22_16410"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HSB16040.1"
                     /translation="MRFETTIQRPVAASGVGLHSGVPVSIRIIPAPPSTGIVFLRTDL
                     ERFPIPANWRNVARVSYATSLMRQGVLISTTEHLLSVFYSMGVDNAYVEIDNLEVPIL
                     DGSGQPFVKMIERAGLRMYRRRRRYLRIRRPVTVEDRDKRISILPADRFLLSCEIQFD
                     HPLVGQQSVEMEVTPGRYASELAPARTFGFSHELDQMRNMGLIRGASLDNAVCFERES
                     VMNPGGLRFPDECCRHKALDLIGDLALIGKPLLGHVIAERAGHAMHAALVARIMSDPS
                     LYEVLSFDQLADRVAHALVS"
     gene            75116..75676
                     /locus_tag="VLE22_16415"
     CDS             75116..75676
                     /locus_tag="VLE22_16415"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSB16041.1"
                     /translation="MSFARAARQLPNFITATRIVLTPFIAVSILGGGHRNALILLVIA
                     GITDGLDGYLARRFQWFSRLGAYLDPVADKLLLAVVYICLGVIGLAPLWLVAVIFGRD
                     ILILAFGGTMMARGGRRELRPSIWGKMSTTFQIATGVALIAGRAYPALALDRAGLWLI
                     PVTAAATLWSGLHYAWTAWRMVRGPA"
     gene            75728..76294
                     /locus_tag="VLE22_16420"
     CDS             75728..76294
                     /locus_tag="VLE22_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16042.1"
                     /translation="MTRYLPSRRYLWGGLAAWGLAALSGVFAIEWAPALIAVVLFLIS
                     SGLLVFLALRPPVEIRESHLIVGNRAIPWGEIRRVDRTGWISPLVVRLTFSDDSRMLL
                     IYPGDLDSANSLLRHLRRHSREAQIDGRAHREFWGDALPAVPERKEELPSPRYRLLRP
                     EDEAEVERMFHQLKTVGRLDQNNSSDEK"
     gene            76298..76897
                     /locus_tag="VLE22_16425"
     CDS             76298..76897
                     /locus_tag="VLE22_16425"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HSB16043.1"
                     /translation="MYFSKPFRSGLAVGGALVAVLATTYPISLGALGGFLVKAEQPVR
                     ADIAVVLAGDYFGNRITKAGELVRQGYVSKVLVSGPKGFYGYAESDLAVPFVVKKGYP
                     AECFIPFPLDADSTREEAEIIVGELRRRNVHTFLIVTSDYHSRRAGSIFRNLAGGLKF
                     RVVAAPDKSFRAEGWWKTRPGQKQFALEWMKTIATWIGL"
     gene            76902..78644
                     /locus_tag="VLE22_16430"
     CDS             76902..78644
                     /locus_tag="VLE22_16430"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB16044.1"
                     /translation="MREALKTVWPYMWRYRRGWARGMGALLLKDLFAAAVPFFIGKGV
                     DSITRGFRLGGILWFAAALVGFSAIKGIFQYWMRVILIGISRDIEYDLRNDLFQNLVR
                     LSSDFYARVRTGDIMARATNDLNAVRMMLGPGVMYWCETCLTFLVVTAIMLAVDWPLT
                     LLALLPAPLISLAVVYFGRKIHARFERIQRMFSDISSRVQENISGVRVIRAYVQEEAE
                     LRQFEKLNKEYIAENLKLARISGLFMPLLQALVGLTLLLVLWIGGRRILAGHITLGQF
                     VMFNTYLAMLVWPMIAFGWVVNLMQRGTASLKRINELLTERPTIAEPASPNPLPEAPP
                     GEIEFRDVTVEYQTGRALSGVSLRIPAGATVAIVGHTGSGKSTLVNLIPRLQDPTRGA
                     VLLDGTDLRELSPADLRRQIGFVPQETFLFSATLAENIAFGVETATPEQIERAAEIAG
                     LGPDILSFPDGYQTIVGERGITLSGGQKQRTAIARAVLRNPRILILDDALSSVDTLTE
                     DRILSGLAEVMRGRTTILISHRVSTVRSADRIFVVEHGEIAEEGTHAELLSLGGYYAD
                     LHQKQLLEEELEAI"
     gene            78700..79560
                     /locus_tag="VLE22_16435"
     CDS             78700..79560
                     /locus_tag="VLE22_16435"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16045.1"
                     /translation="MHFLRLLPALAATLISCTPTAAFGQQMTTPEGVSILRNKSFDDS
                     DRARKEILDLYRDLRVTDVLDGMDLIGLQDIGLMDNEIRPLWRDTDSFTHRFVGLAVT
                     VRHVPSDVRVGQNSFPDLAGFLKFKDEQYGRAPDAWLKTARQGDAAVIDASGVFETGF
                     IGSNNSLGWAEKGVVGVVTNAGARDTDEIVKVKRIPVYCRNGYSTRGVRPGRLIAESY
                     NFPVNCAGVTVFPGDVIVADGDGVIVVPRQAAIQVGKLAREINVDDEQGRGKRFERLG
                     IPHDSTVKVK"
BASE COUNT        15051 a        24595 c        24761 g        15291 t
ORIGIN      
        1 atttcgatcg gatcaacccg cttcacatcg ttctctacta tcccgacgga ctcgaagccg
       61 tgtcgcctgg caagctcgat ctcgagacct cctactattt tgagattgac cccaaccgcg
      121 ccggcaacgt tttctcgcgc agtgagtggg acaacgaaca ggcatcgtgg ttctccttcg
      181 tcacgcgtta ctcgaacgcc aaccacacgc attacgacat gaactcgttc ctgttcacgg
      241 ccttcggaga ggctttcgcg acgcacgcca acatctttcc atacagccac aagcaccacg
      301 gcgtggattt cgagcacaac atcgtcatcg tggacgaggg tggcatgccg gccaacgacc
      361 gtcaatccgc cggcgacgac ggttcggtga agggcttcat gaccggcttg ggcttgggcc
      421 atttcgccga ctatgtgcgt ggcgacgccc ggctctcgta cgccgaccgt tcgatccgca
      481 catcgactcc ggctttgcac gccaatcgaa gcgtcctgtt cgccaaacaa ggaccaaacc
      541 cgtacgtcgt gatcaccgat gaaattcaaa aggacacggg cgaacacgat taccattggc
      601 agtggtacac ggaggcgaag gcgatcgcag gccagggaac cttcgacgag ccgttcgtca
      661 ttgagggcga gaacgcccgg tgccgcatcg cgtttctaga tccggcggcg ccctcgcatg
      721 acttccgcat cgcaaagggc gggagtgaac ggcggccact cgaactggga ttgctacggg
      781 tcaatctccg gggtgttcag gcacgctatg tggcgatcgc ggccgcgcac aggaaagatg
      841 ctccggttcc gagaatgtcc cgcggagcgg ctgttgaagg caatccctcg gccgccagcc
      901 tgatcgtgga gggcgaagcg tttcgcgacg tgattgtctg gcagcccgag gaagccggcg
      961 gcagccgcgg gcggccgatc tcggccggca agttgaaaac cgacgcgctg ctcgcgatgg
     1021 tgcgaaccga ccagaaaggg aaggtgatcg gttatctgat ggcagacggc gcgtcgctcg
     1081 tattctccgg gaaggccctg gcgcgcgcgg acgctccgtt cagtgtcagc gccgacgcca
     1141 agcagacagt aaccacgggc gcccgccgcg cgcgtgaggg actgccgccc cttccggcac
     1201 cgagggccac cctcgtgaag ctccgttgag gatgtgacct ctactcattc cggtcgacgc
     1261 cgcccgccag ggagttctgc tgaatgttgg aagcggttcg atggtagcgc taaggggatt
     1321 cggccctatt cgcgccatcg tggccattgc cggatcatgc ctagcgcttg atctctaatg
     1381 tcgtcttaat acggccgtga tggctgctgc cgttgtgttt ctcttccgtg gcagcgcgtt
     1441 ggcggcgcat ttgggcctta cggcggatgc cctcacgtcg acccgcggcc ccgctgccaa
     1501 tgggtcgttc ccaccaaaag cggtgggttc cgtgtaaccc gtacgtattg aaccgcgggc
     1561 agtgtgaact atcgcgtacc ggcaggaagt gccggagaat gctgctcgcg ctgggaggtg
     1621 ttcaacgtcc agctgcctcc gataccgtct ctcttgggaa gtacttcctg taccacgcga
     1681 tgctccggtc aatgccgtcc acgatgtgct tcggttcccg gagcccgtgc ccttcgcgtg
     1741 gataccgcac catcacggcg tccacgccta catccttcaa cgcgatatag aactgctcgg
     1801 attcggtaat gggcacatcc gcgtcgttct cgccgtgcaa caacatgaca ggcgttcgca
     1861 cctgcgccac atgcttcagc gcggagcgtt cccacagcac gtccatcagg ttgccctggt
     1921 ggaccttggc accgaattcc atctcctcgt acatgttgta gtaggtcgtg tagttgtagc
     1981 tgacgaggtt gatgatcggc gccaacggga tggcggcttt gaaaatgttg gtttgcgtca
     2041 ccagccacgc tgtcaactgg cctccgtagc tcccgccttc gatacccatc cggtcgcggt
     2101 ccagccagaa gtaacggcgg gtggccgcat taactccgta gagcacgtcc tgtgcctcat
     2161 tgccgttctg atcgccgaac acaccgtccg caaacttctg gccgtatccc gtggagcccc
     2221 tgtaattcac catcagcgtg gcccagccct gtgcggcgta gacctggttg tgaaagttga
     2281 agccggggcc ttgctgtccg tgcgggcctc cgtggatgac cacgatcaac ggatggcggg
     2341 acgtccccgt catgccgaga ggcttggtga ggaacccttc cacctcgaat ttgaagtcgt
     2401 tgctgacgaa cgtgaagcgc tccaccgggg cgatctcctt gccgcgcagc acatcggcat
     2461 tcaggtcagt cagttgctta gtgacatcac ccttcctgag gaaaagttcc acctcgtcgt
     2521 gagagctggt gaaggcgtag gaaagcaggc cgcccgccgt cagggaccat gaccttacgg
     2581 ccccgcgctc cgcaatcact ctctccgccg gtcccccagc ggctgggatg cgacacagca
     2641 atacgctgcc tcgttcctgc acggtgaaat acacgaattc tccatccggt gtccactccg
     2701 gcggaccctg gcggttgtcc accacggtgc ccacttcgcg ccgtccgctt ccatcggagt
     2761 tcatcaccca gacgtgcgtg tcctccatcg tggtttccag gtcggtcagt ccgcgccgtg
     2821 ttcccagaaa tgcgatctgc ttgccatccg gtgaccagcg cggcacatac tcggcgctct
     2881 cggtcgcggt gatgcgccgg atcgttccac cggcggcctt cactgcaaac acgtcggagt
     2941 tgaagaacag atcctcgtcg ggcgagggat tggagacgaa cagaatctcg cggccgtcag
     3001 gcgaccagtc aatggagtgc tcgtagtggt tccctgtcgt gagttgccgc accttccccg
     3061 attccagatc aacgatgaag atgtgggacc gacggttgtc gttgaagtgc gtcagccctt
     3121 ccgtgtaatc cggcttgtac aggtaccttg tgatcaccac tggatcgccg gatgcctcgg
     3181 ccgtttcggg cccgggcaaa gtggaaacaa acgcgatctg cttgccatcc ggcgcccagg
     3241 caaggcggtg gcctggctcc tgcatgggag aattcgtcca ctcgagcggg gcaatccatc
     3301 gtgccccaga cccatcggga cggcagagaa tgagacccgc cttttcacca agcttgccag
     3361 aatacgccag ccaccggccg tcccgcgacc actcgaccat cgacgattcg tcttttccct
     3421 tggcaaggac caccgtctgg ccgccgttga gcggcatcac ctgcacctgc tcgtacggcc
     3481 ttcctccgcc gtcgcggtgg ctcacggtgt aggcgacgta cgctccgtcc ggtgacaaac
     3541 gaatctcccc gacggatcgc agccggtaca ggtccgcgct ggtcaggcct gccgccagcg
     3601 ccgcgtgtac cgtcgggccg agacacagtc caagagtcag gagagcgagt agatgcttta
     3661 tggcgagaga atctccggca gaatggctcg tcgattcgag gattgccatt ttggaagatt
     3721 ggcgcactcc gtaccgcatc gtttcagggc tcctggatgg gagtaagcgc atgaaagcga
     3781 ttgtacgcaa gaacaccggg cgctcggacg gcctggttga tatagaggcc gccctgcggg
     3841 cgctctcgga atgaagaatt tcgagaagaa cagcgtacca gcggaggcag tttggcgtac
     3901 aatactgcca tccgggaagg caaagaatcc cagtccgtac acggtacagc accttatgtg
     3961 gagggaaggt gacggaaatc gaagcgatac gcaacatggt tccatatggc gagcgcccgc
     4021 tgttcgataa gctggtggag gagttgccgc tcgtcctgca tgacaagaag aaccactctc
     4081 cgggagtaca ggccgcgtgg gtggcaaggg ccgtagtgct ggctctgccg atggactgta
     4141 gcagacgtac ggacgagaac tgctgcgggg gctgcctcta ctggcgtaag agctcgtctg
     4201 ccggggcgtt cggtctctgt gaccgtatca gctttggcgg gcacgtacac ggcgacgtgt
     4261 tagcctgccc ggtagacacc agagacgacc cggcctcgct acgcaccttg ccaatcttcg
     4321 ggtgtgtgat gttcaaggtc cggtcctgac acgtcacgag gcgaaaatac gattactgat
     4381 tccatcgggg gatgcggcaa tcacaacggc ggctgagcac gaaaaaggcc cagtgaatcg
     4441 gagggactca ctgcaaggct gaaaagataa gaggccgtcg gcgcggaagg cgatccgatg
     4501 gcccctattg aggtagggcg cttcccgaag ggacagagct gcttgtctgt gacacgccct
     4561 acccacgcca gtgttcaggc atcagagata atacgcgcct aaagtccgtt tttcaagcac
     4621 tttttcgaac actgaggggc ggcctcggtc gatccgtttg cgggggggtg agcagacttg
     4681 gggagaggct gacaggcacg aaaaaggcca gtgaatcgga gaagttcact ggcccgctcg
     4741 caatcataat cttcggctac cgggctaggg tccgatggcc tcgtgagggc aaggcgtagg
     4801 aattaccgga ccggtttggt ctctgtatga tccaccctac ccatgatgcg ccaaaattcg
     4861 cgcaccacgg atattgccct ccttgagtcc gattgtcaag tactttctca gaagtgagag
     4921 caactttctc cgggggtgcg gccgacgtgg aaagagaacg ggaccaccgg cacgatgagg
     4981 atgaatgatt ccacgctcac cgcacccgaa gccccacacc gggttagcgc taacgggatt
     5041 cgaacctgat ccaccctcgc cttgcatgtt cgcaccgcat ctaacgtgtt gtatttgcgt
     5101 acggctggta cggactttca gcgcgctaac ccaatcgcag agaccagaga ccatagaagg
     5161 cgcagccgag ccctgtggtt cgtctggcta gtcggaaaaa cgccgccgag gatcgcggaa
     5221 cagcccctgg gcgccgggtc tgggaggcgg cgcgatggcg cagtccggta tgatgaaggg
     5281 catggcagaa gtggctgagg aggccgcgaa actcgacggc gcgccggtgt tgcagatcac
     5341 ccgcatgggc tcgtcggcgc aagcgatatc cgatttcagc accgcccgag gaggctctgc
     5401 cccagcaggc gcagggcggc ccggcggcgg gtctcctgat ggagaccacg accaagctgt
     5461 ccggattttc ctccgcgccg accgaccagt ccaggttcga ggtccaggcc ggtttcaagc
     5521 aggtcgaggc cgagatgctg aaggagaccc ggaggtaacc gcgcaatgac caggcgtggc
     5581 gctctgatcc tcgtgggcat cgccctgctg gctgtcgccg ccccgcgccg gcggccggcg
     5641 atgccgaaaa tcacgcaacc cgtgctcttc ggcacgccgg aggccgaccg catcctggcc
     5701 gccctccagg tgtttccccc ggacaatccg tggaaccagg atatttccac cctgccggtc
     5761 cacgcggatt cagctcgaat catcgcctcc atcggcgccg ggaagaccct cggctacaac
     5821 ctggatatga acttcgtgat cgtgccgccg aaccagaagc ggattccggt gaagatgctg
     5881 caatacccgg ccgagtccga ccccggaccc ttccctgtgc ccgacaacgg ccccatagaa
     5941 aactggccct tggccgtgaa tgaggacatc aaggccgttg cgcgtcccgg ccagacgcta
     6001 gacgacattc agcgccacgg ccggggcgac cggcatatgc tggttgtcga cccgggcaac
     6061 ggaaagctcc acgaattcta cgcggcgcga aagaccgacg cgggttggga ggcgtccaat
     6121 gtcgccacct tcgacctgcg aacgggcgct ttgcggcccg aaggctggac ttcttccgat
     6181 gcggcgggac tgccggtctt tcctgccacc gtgcgctacg acgagtgcga gcgcggcctg
     6241 gtggagcacg ccatgcgcgt gaccgttcgc aggagccggc gcgcctacgt tcttccggcg
     6301 acgcacttcg ctagccggct taccgacccg gatctgcccc gcatggggga gaggctccgg
     6361 ctgcgccgcg atttcgatac ttccggattt ccgccccacg cccgggcgat cctcgagggg
     6421 ttgaagagat acggaatgtt cgtggccgac aacgggcagg actggctgat gtccatctcg
     6481 cccgatgcgc gcctgaaggg gctcgaaact ctgaagcggg tcaaaggttc cgacttcgag
     6541 gtggtcgaga ccggcgccca ctccggcagt gagcggccag ccaagtaggc tgagcgcggg
     6601 ggcgcggttg agacattggc gagttcccgc ctactcgcgg tgaggagaac gtaccaggct
     6661 tcttccggga accggaaacg tatcccgtcg ccagtgcgct cgaactgcgt ttcacccaaa
     6721 gtgattgagg agtaatgcag gaaggtggta gcgctaacgg gattcgaacc cgtatttgag
     6781 ccttgagagg gctccgtcct aacccttaga cgatagcgcc attcttcaat cagtatacag
     6841 cagcggctgc gcggcgcgtg ctccgcggta caatcgtgcc gatgaaatgg cctttgttcg
     6901 cactcctcac gatgctcgcg gccgctcttg cctggagcgg tgtacgctcc cccgagatcc
     6961 cctttgaaaa ggtcacgatc gacctcggcg ccgccgaaac ctgcgccttc accgacatca
     7021 acggcgacgg acgcctcgac atcgtctccg gcgaatactg gtacgaggcg cccaagtgga
     7081 ccccgcacaa gttccgtgac ctcccctatt ccaacaatta catggacgcc ttcagcgacc
     7141 tgccgctcga cgtcgacggc gacggacgcc cagacatcgc taccgtcgcg tggttcggac
     7201 ggaagatcaa ctggtttcgg aacccgggtc ccacaggcgg cgcttggcgg gaacaggcca
     7261 tcgaggacgg attcaatgtc gagttcgctt tcctggccga tctcgacaac gacggcaagg
     7321 cccgcgagat tctgccgcag ttcggcaatg agaacgcccc gctcgcctgg tacgaggcgc
     7381 gcaatggcgc gttcgtccgc cggatcgtcg ctccaaagag ccagggacac ggcatcggag
     7441 cgggtgacgt caacggcgac aaacggaacg acgtagtcac cccgaaaggc tggtacgaag
     7501 caccccctga cccacggacc ggcgagtgga ccttccaccc ggatttcgac ctgggctcca
     7561 ccggcttcat ccacgtcttc gacgtcaatg gcgacgggcg caacgacctc gtcacctcgc
     7621 tcgctcacga ctacggcatc ttctggatgg agcgcaccgc cggtggccag tggaagaaga
     7681 acttgatcga cgaaagctgg tcgcaggcgc acgccctcgt acttaccgac ctcaacggcg
     7741 atggccgtac ggacctgctc accggcaagc gttacatggc tcacaacggc aaggatccgg
     7801 gcgagcggga accgcttgga ctgtattggt acgaataccg cctatcgaac ggcggcaacg
     7861 tggagtggat ccggcatgtc atcgactaca gcacgcgggc cggaggtggg atgcagatcg
     7921 cggtcgccga tatcgacgag gacggggacc tggatttcgc ggcgggcggg aagagcggct
     7981 tgttcctctt cctgaacaga acgagatagc gtacgacctc ccgcatgtgg cgcgcggaag
     8041 gccgcgggcc acatgttggg gggagatgag cctatgccac cacgggctcg aaataaccgt
     8101 gcgcaagttc gggcttgatc tcgagcgcgc ggcgccagaa agaacgagct tcatcctcct
     8161 ggccgagggc tttgagcgcg tgtccgaggt tgagcgccgc ctcggcgaat tcgggctgga
     8221 cggccagcgc ctcccgatag agacgcaccg cctcgtccat gatgccggct ttctgcatca
     8281 gcaggccggt gttgtagaac aactcggggc cgcgctcacc gagttcgatg agccggccat
     8341 ggagctgcag cgccgcctgg tagtcgtctg actcaagcgc catcgccgca aggccgcgca
     8401 acgcgtcgat cgaatcgggc tgcatcgcaa gcacctggtc gaacgccttt cgggcatcgt
     8461 ccgttctccc gagtttccag taggcgatgc cgagattgag cagcgcttcc ggccagcggc
     8521 cccgttgctt caggcaggca tcgaatgcgt cggcgctgcc ctggaagtcc ccgcgttgca
     8581 ggcgcaggta gcccatgcgg aaccgggcat ccacccactc ggggtttcca tcaagcagct
     8641 tcacatacag cgcctcggcg gtgtcggggt cgccggaaag ttcgagcaca agcgcaaggt
     8701 tccagcttgc cgccgtctgg ccgggatcga gttgcagggc gcgttcgtag gccttccgcg
     8761 cagacggcag gtcgccgagt tcctgctgca cgataccgag gttgacgtgc acctgcggcg
     8821 agtcgggccg tagccgtgcg gcctcggagt acgatctggc ggcgtcgtgg aaccggccgg
     8881 ctttctgatg cgccacgccc agattgaacc acgcttcgaa gtgattcggg gcgatttcaa
     8941 cgatcttcgc gcagtagcgc gcggcggatt cgtgatctcc gccggagaag gccgatgcgg
     9001 ccaggccctc cagcgcggtt tgggagttgg ggcgcaccga gagaagctgc tcggcggtct
     9061 cgcggacgcg gtccgaatcc ttgcgggcca tgtcgacggc gatcaggttc acgagcgagt
     9121 cttcggaagt cgggttccgc tggaggatcc gccggtattg gtcccccgcc tcgtcgaacc
     9181 ttccagccag ttgcagggcg acggccttgc cgaacatcgc cgcctcctgc gtcggcatct
     9241 gattcaggca acggtcgaag gcttcgagcg cgggctcgag ctgccccaac tggagattac
     9301 aaatgcccat gccaaggtag gcttcgttgc ggccaggatc cacttcgagt gaccgctgga
     9361 atcgttccac agcttcctgc cagcggccct gcttctcgag gcacacggcc agattgaacg
     9421 aggcggtccg atgggacggc tccaactgaa gcagcttcca gtaactggtg gtcgcttcct
     9481 cgtactgctc caactgaaaa tgaatgtggc cgagtgcgga gtaaagttcg gcgtcttttt
     9541 cgccgctatc caccgcgctg tacaactcat tcagcgcttc ttgaggcttg ccctcgaggt
     9601 gcagggacac tgctttggcg agagcatgtg acgccccacg cctctcggat ttcgcctcag
     9661 ccacgttccc cacggcgccg gtaccgggag actgcggctg gttggccggc atttttcttt
     9721 gactcatggt agattcccac tccttaacat gatcggattc gggtacatgt aggagcacag
     9781 cctgcccaca aagtcgtcct ggtgtgacag cttcacccag tagagccgcg cccgttcacc
     9841 cgtttcactg aaaaaaccga cgccgccctt ctctaggcgg tcatcgcgcc aatgatcgat
     9901 aagctgtccc tcaatcgagg tgctgaaatc tctccccctg acggccactt gcaccctata
     9961 cggcgtgtta ttgtgcatca acattcttat cggaacttct gtatgttcac cctcaactcc
    10021 gttgactacc ggatagtgga ccaacgccat ttgcggggcg ggtcccgcct ttacgacagt
    10081 cagtttggct gcgtagtagt tcatgccgtc tgctgcgcgg aatacccagc tcacggcctt
    10141 tcggtcaatc tgggccagaa actcaatccg gtaatcctgc atctccacgg atgggctgta
    10201 aatggcgagc ttgcccggcc ggatgaagcc ggaatcgtcg aatgtccatg atttcaacca
    10261 gtttccggag cctgcccatc caccaagact cccgccttcg aaattgtcca ccaactcgat
    10321 ggtggcccgg tcctggattg atttgcggac attggcccag tgaccttcca cgacggtgcc
    10381 gacacgcctc gggctggcga cccagagcat catcagcacg cagaagctgg cggtcgcgaa
    10441 ccgtagatag gccggcgtgt tccgccacag ccgaaggatc gcctgccacg ggtcgagttc
    10501 cggccggtcg agtggacgga ggtattcgaa cgtctcgacg atgcgaagtg agcttgttct
    10561 cgggatggcc ggcgtccgga ggggcatggg taattccatg tacgccagcg gcacccgggt
    10621 ctgatccttc ggtcgcacac cgtcaaagtt gattaccgtt ccgcgctgta ctttacccgc
    10681 ccgcgagtac cggaataggg acgccagcga cggcaaaacc gacgaagtgg ccgcggccac
    10741 ggcgccgtgc ggggcatcga gcggcacggc ggcggcgttc agcgcacgca cctcttccgt
    10801 gcgtgagccg attgtgccca gcgcctgccg aaccgggatc ggctccacca ccggaaatct
    10861 ggccatcgac ggacggaacg gggctggccc cacgccggcc gatgctccgt acttcggagc
    10921 ggctgccatg acggccagcg gcgtcaatcc agccatcggg aagctggcga gacccgcatc
    10981 gggagccgtc gcttgggttg ctgcagccgc cgctgccgca cgcgcaactg cttccgccgg
    11041 caaggcaacc gggctgaggg ccaaccgcgg cagaagcgtc agcgccggcg ctaacggcac
    11101 aggatgggcc tcgacggcga tcatcgcggc ggcgggctcg aagaccggca agcgagccgg
    11161 ttggaatccc gctttcgtga ccgagaaaat caattcgatg ccggatcggg ccgaggccag
    11221 cgtcggaacc atcgaccagg ctcgccattc ggccgttgtg tcgggccggc aggccgccag
    11281 cgccggctga gccgcgggcg ccacagggaa ggctcgttcg cagatggcgt ggctggtcag
    11341 ttcgacggct ggcggaagct ccagcggggc caaatggagt gcggctgcat gcttccatac
    11401 gacaggtagc gcgaacggca atggatccaa ctccgcctgc cggacggctg cccaggaatt
    11461 ggccgtggcg gcgtttggag tctgtggaat gggctgcgcc agggcgagca aaggccgcac
    11521 ggccgggcct gggacgggcg gcatggcgac agcaagcgcg agttcaaata taggcgctac
    11581 agaggcgacg tccggggtcg cggacgacgg actgggttgg gcgggagcgg ggcgcggaag
    11641 cgaaaatacc gcctcgcgca aagtgggagt gctcccctgg cgcagcattc cactcaaaaa
    11701 ttcaaacctt ggagacgggt gaggaagctc actcggctta aggatggctc ctagcgcgag
    11761 cccgggcgca gtgggcgcaa ccggatcgag ccgtccccag atcggcgcgg cattaccaag
    11821 caacgggagg atgggtgagt gagagaaacc cggcagggag ggcccgggaa ccccttgcat
    11881 cgtttcgtcg gctgcctgaa ggtgtgctgg aatctgctgg gggcgcggaa agtggcggag
    11941 aacgttcgag taatctcgcg gttttcgacc gtcgcttgga aagaaaatcg acgagacagc
    12001 cgaacgcgca gattgcactg cgttattgat ctcaggctcg aggggaaaga cctcgcgcgc
    12061 gaggtcggtt ttggtctcct gcagcctcgc gagcgccatt ttggtgagct tcttgtaacg
    12121 ctgccgatgc tggccacagc agaattcgcc gtcggtgagt tctttgagga acccaagcgc
    12181 ctttccgcaa taaaggcagt gcatggctag atcacacgct cagaggcgaa ttctgcgcct
    12241 catccgtttc caagagccca cggagcctcc ctcggcccca aaattcggat ccatgttcgg
    12301 tcgtcgcagg gccgattctc ctccccgcga cgctcctcca tccgtgttcc caaatgtaag
    12361 gtctttccac cagggtagca agaccgaaat taggtagcat actcctgtat accaaggtta
    12421 atagtagttc aatcggcagt cccagccact ttggctacat acgcttacgg ctattatagt
    12481 acgttttgag ctctagttcc ggcgcgaatc acccgtatga gaaacctaca actagcagtc
    12541 agtccggtcg acttgaacgt cggaacgcga aaagatggat ggattgaggt cgacaccgag
    12601 acccgggcca ggcggcagtg gcagtgcacc gccggccgga actagggagt gcgtcaggat
    12661 gccggcatat tcttcggcgt agtgctgccg gaccgtttcc aggatcgcga ggttcgtcag
    12721 attggcagcc acgtgggcgg aagctaggtg cagcaccggc ccaccacagt tgtgcggtgc
    12781 aaccggcagg tagtgcgtat cggcgagtgt ggcgatgcgt tttgcctccg agatgccccc
    12841 gcaccagcag atgtccggca tgacgatgcg ggcagcgcgg ttctcgaaca actcccgaaa
    12901 cccgtatcgc gtcatgagtc gctcgctcag gcacagcggc agcctggtcg ccgaggcaag
    12961 ctcccggtag gcggccaagt tgtcctgcgg cagaagttcc tccagccaca tcggctcata
    13021 cggctccagc gcctgggcga tccggatggc gctcggcagg ttccagtagc catgaaactc
    13081 catggccacg tcgacagaat cgccgagttc gtcccgaatc cgccgcaacg ggtccaaccc
    13141 tcgcctgagt tgatcggccg tgatgaagtt ccctccggtc tccttggcga ttggatcgaa
    13201 cggccagatc ttcatcgccc gaatccccac atcgagcagt tcccgcgcga ggcggaccgg
    13261 ttcggtcatg aagtccacgt ggtcgtagca cgtattgtag atccggatgg cggagcggga
    13321 cgcgcccccg agcaattgat agatcggcgc gcccgcgacc ttgcctgcca ggtcccagag
    13381 ggcaatgtcc acggcgctga tggcccgcat ttccgccccc gcccagccgc tgtaggccac
    13441 cgcctggaac atgtcgctcc acaggcgatc gatccccgac ggatcgcgat cgagcagcac
    13501 cggtgcgagg gtacgatgga tgaccgcttt ctcggactcg ggacgggggt aggtctctcc
    13561 taaacccgtc aggccggaat cggtatgaat tcggacccac agccactgga tggggcctgc
    13621 gtgtacagtg atcccacgtc ccaggtggat ggtctcaatg gctgtaattc tcatccgcga
    13681 gaaagtgcag cttgcgaccc aggtgcgaaa gaagttacaa cgccggcgga agggccagcg
    13741 ccgctttggc gagcgccctc tgcaacatgt cgatggccga ggcctcggga attccgcgcg
    13801 aggtcgagag ttcggtgatc aacatgtgcc tggcgcgatc gagcatcttc ttctcccgga
    13861 acgagagtgg cttgacgttc tggaggagaa gcaggctctt cagaacctca gccacctgga
    13921 acatgtcgcc gacgcgcatc ttctcggagt tctccttgaa gcgatccttc caatcgccgg
    13981 ggcacttgca ctttccgcag gccaggaagt cgagcatctt ggcaacctgg ccgttccgag
    14041 tcaccttgcg caacccgatc tcgtccacgt gcgagaaagg aaccatcact gtcaggctgc
    14101 tgcacatgag gcgaaggaga taaaagcgct cggcctgggt cccaaaggat ctgatgctga
    14161 tattctcgat ggtcgctacg ccatgatttg ggtatacaac cttttcgccg atctgaaaga
    14221 gcataggcaa ccctgccggt gtgtacatct tcctccaggt aatgggtgcg ctcgaaaaac
    14281 cgaaagaaaa tgttaaacgg aatgtgaacc gatgtcaacg aaaaaagtcc agcgaaggcc
    14341 cgaaaaacgg gcacttggag gcaactacga gaagcaagaa taagcggaca aaagaaaagg
    14401 tgtggaagcg ccggcaaaca gttccacctt tgccacttcg atcaagtggc tgagcgagac
    14461 taaagctgtc tcagcgaccg cccgccgtga tcagcccttc cggatctgtt cgaccagccc
    14521 ccggacgtac tgaagattcc ggcgcatgtc gttctcgatc gaccccgatg gcgaggacgt
    14581 ttcgaggttt gcgaatccgg tatagccgat gtccgcaatg gtctgcatca cggcggggaa
    14641 attgatctgc ccttccccga ggtagtttct gccgtctttc aagtgaatct ggcagatgcg
    14701 gtccttcccg agccagcgca tctccttcag gatgtcgaaa ccggcgttgg tggagttgcc
    14761 aacgtcgtag taaacgagta gagctttcga cctggcacgg tccatgatgc ggacattgtc
    14821 ttccgccgaa atcgtgttct ccaaggcaag gacgacgcct gccttctgtg cctccggagc
    14881 aagctcccga agcacgtcgc ctacgtagtc catctcggcg tgcgacttca aggcgccttg
    14941 gccgaagaac ggcagcagca taacacgcgc cttaagattc ttggttgcga gaataccgtc
    15001 cgatatccac ttctgcccga gcttatcgct cttaaggtag ttgacgtgga ggatgtcgag
    15061 acaggtgccg gcgatgccga tcttgcggct cttcgcttca gcgacgtagc gttgctgcag
    15121 gccggcgtcg gacaggggca accggtcacc ctccggcttc cggcccaggc tcacctcaac
    15181 tccgtcaaat ccgagcgcgg ccgcaagaga aagcgcttcc agcttgctgg tctggcgaag
    15241 gttccagtcg gtaacgccga tcttcaattt gccgagcggc ttcggacggg ccgccagaac
    15301 agccggagag gcacaaagca ccgcgctgga ctgaaggaaa catcgtctgc tgatcgacat
    15361 ttgcgttccc attaaccgca gagggatatt atagtccgat ttcgtgagag cgggtgtagc
    15421 ccgcgccagc acgggatctt gggcggctgg cgcatcgccg ccgaggaccg agcggctgaa
    15481 accggctatt gtcgcgcaag ctccgtgctg gttgggccaa cggctcctac cataagtccc
    15541 aaggccaacg tctgtttcct gaatcccgga atcggggcag acgtccaaat gaatacggga
    15601 caagtgggca atttgtgacc agaacaacca atccgaatac cgccctcgga cctgccgcgg
    15661 ttgccagcgg atacggaaag aaacttcacg ttaagatctc gcaatattcg atgcgctttc
    15721 gcactacgaa ccgctgtgtt ctcgcggtgg ttgtgcgctc tgggtgattc tgctcaggaa
    15781 ggggggaact tgagatgagt ggacttcaga ctggttcagg cttggccggg ccggacgcga
    15841 ccttccggct cgatgtatca ctgcacgact tactcgtgaa gcatccgcca aaaccggcac
    15901 ccccgcctcc gcggacggcc gaaccgcgtg ccctgccgga tccggttttc ccgcagcatc
    15961 ctgagctgct gggtaaggag gtggagcctc ctcctcgcga tccgaattcg ccgtatgggc
    16021 gccgctggac gcccgcgctg atctaccgcg ccttgaatgg ctggctgttc ccttacgtta
    16081 agtcccgagt gctgccgggt gactttcacc ccatcattgc gtaccttttc accgaatgga
    16141 aatgtaacct ggattgccat tactgctggg ctttcgacaa cagtgtcaaa ggcatgtcgg
    16201 aagacacggc ccggcgctcc atcgactggc tgcactcgac gacctgccgt gtgctggctc
    16261 tgatgggcgg cgagccgttg ctacggccgc aattcgccca taaggtcgtt tactacgctg
    16321 caaagaaggg cttttgggtc tacgtgccca ccaacgcacg gctgctgcga ccggaggtca
    16381 ccgatcgcct cgctgacgcc ggcatggcgg tcgtcaactt tgccgtggat gtcgtgaacg
    16441 agaagcctgg gctcccgaag gccttaaacc cggtgcggga atacttcgag tacctgatca
    16501 gcaagcagta ccaatacggg tacatcgtct tctttaacat caacatctgt cgcaacaatc
    16561 tggaggacgt ccagaagctg accgaaatcg cgcgtgacta cggcatcatt accgactacc
    16621 acatcaacga gtcacccatg atcgagcaga gccatttcca gcacatgaac gagaacgaca
    16681 ctttcatccg gcgggaagac tggccgaagg tggacgcgct ggtggactgg ctgatcgata
    16741 agaaccgcgc gggttacaag atggtcaact cagccaagcg gctggcagac atgaaggcgt
    16801 tcatgcgagg caaagtggaa ccatggaatt gtcgcgcggg ccagaattcg atgatcattc
    16861 gcgtggacgg gaccctggcg ccctgctttc caatgtactc ggcgacctac gattggggca
    16921 ccgtcgagaa ccatagattt gaagtaaagc aactggacca gatgaaggag tcctgccaga
    16981 agcactgctt ctcgacgctg aatcacaacc tcggctactg ttacaacgat gcgcgggtgc
    17041 tccgctgggt aacgaggcag gcattgcgcg gcttccaggg cgtgagcgga agcttcgatt
    17101 gaagctgcgg actgagtcag gtcagtggtc ccggtcggtg acgagctcgg taagcgcaaa
    17161 aagcgcgcgc tggtacggcg attccggaaa acctcggatg atttcacggg acttcgccgt
    17221 gaaagcctcc gcgcgttcca tcgtgcgcac aatactccgg cggcgttcca tgaactccag
    17281 gattccattg aacggcacac ggtcgtagct gccgtcgcgc agaatctcct ccacctgtga
    17341 tctttcctcc gtgctcgcgt tttcgagtgc atagatgagc ggaagggtca ccttaccttc
    17401 gcgcaagtca ttgccaacgg gctttccgag cacggactcg tcggccgtaa aatcgaggac
    17461 gtcatcgacg agctggaacg ccattcccag gttccacgca tattcaccga ggcgcgcctc
    17521 gtccgcttca tccgcaccgc cggttacggc tccgagccgg gcgcaggcag agaaaaggct
    17581 ggcggtcttg cggtcgacca gatccatgta gtcggcttcg ctcacatcga cggaaccaat
    17641 gcgcccgagt tgaaccagtt ctccttcaac catcatctgt gtcaagccga tgagcacatc
    17701 aaggacgtgg aagttgcgct cgcggagagc gacctggaag gcctgcatgt acagccagtc
    17761 gccggcaagg acggagatgt ggttgcccca aagaacattc gtagacgggc gcccgcggcg
    17821 cgtctcagca acgtcgatga cgtcgtcgtg cacgagggtg gcggcgtgga tcatctccac
    17881 gaccgctgcc agccgaatgg cgctttcact gccgttgccg aacagtttgg aggccagcag
    17941 aagcaaggct ggacggaggc gcttgccgcc gctcgcatgc aggtaacggc caatcgccgt
    18001 gatcgcatcg acggaggcga cggactgaag ttggatctcc cgctccacca actggaggtc
    18061 ctcccggacg agatccagca gttcccaggc ggtaaagggc tgtccgacct tgctcaactc
    18121 catggaatcg tcccagttta ctcgctgccg attgcctctg gcaaacacaa ccgcctgaaa
    18181 ttgcctgtgg tagctccagc cagctcctcg atcggtatct ccttcaattc cgccagcctg
    18241 cgcgccgtat ccagaacgaa caccggctcg ttccgcttgc cgcgatgcgg aacgggcgca
    18301 aggtacgggg catccgtctc cacaaggatc ctatccagag gagtgaactt cgctgcctca
    18361 cgcactttgt ccgctttcgg aaacgtgatc actccaccga atccgagata gaagcccatg
    18421 tccagcgcct gcttcgcctc ggcggggccg ccggagaaac agtgcatgat tccgctggct
    18481 gtccaactcc cgcggagaat cttcatcgtg tcctcccagg cgtcccgagt atggatgatc
    18541 acgggcttgc cggtttcctt ggcaatccgc aactgctcct cgaaagcggc ctgttgttgc
    18601 tcccggggcg aattgtcgta gtagtagtcc agcccgatct ccccaatcgc gagcaccttc
    18661 ggttgtgcgg ccagttgcct caagcggttc catgtatcgc cggccgcttt gctggcgtcg
    18721 tggggatgca caccgacggt ggcgaagatg cgctcatgag tcgaggcgac tcgaagtcct
    18781 gcttcgaggt ccggtggacc ctcacccgtg cccactgtca ggatgacttc aaccccggcg
    18841 ttggtagccc gctcgatcac gagttcgcgg tcgccgtcaa aacgcgggtc gtcgaggtgg
    18901 cagtgagaat cgaccagcgt cacttcaaac gcgctccgac ggggatctcc tcgtgaaaac
    18961 ccgccaacac cggtttcccg ccctcgaccg aagccgccac gatcatcccg tcagatggga
    19021 tgccgcgcaa cttgcggggt tgcagattcg ctacgatcac aacctttcgc ccgatcaaat
    19081 cctccggccg gtaagcttcg gcgattccgg ccacaatgct ccgcggctgg gcctcgccga
    19141 catccaccga catgtgaagc agcttatcag cgcccttcac gggttccgcc gtgagaacct
    19201 ttcccacacg catatcaact ttgacgaaat catcgatcgg gatcttgtca gacgcctgcg
    19261 gctcggccgg ggccgctcgc ttacccacga ggatctcctg ccggatggtt tcttcccgct
    19321 cgagcgcgcg catcttctcg acggactgct tcagatcgag ccgcgggaag acagccgaga
    19381 tttcgccgat ccgctggccc tcctgtagtt gtccccaggc aagccctacg agttgcaccg
    19441 cctcgagatc gtcgcccatg ccgagctgcc gccagatttt cgccatcgag cccgggatga
    19501 ccggggccag aagggcacag gagatgcgca acacttcggc cgccgtgtat agcgtctcat
    19561 cgagcttcgc ctgcgactcg gcatctttct tcgcggcgag agtccacggc gcctgagcca
    19621 cgatgtactt gtcggccgcc gcgagcaggg accacacggc ctcaagccct ttcgagaagt
    19681 cgaaattgtc gaatgcctcg acggccgcac ggatcgtccc gccggctagt tgcgcgattt
    19741 cggcgctgcc gcccgatgcc ggtatggcgc cgccgcgata ctgccggatc atcgtcaggg
    19801 tgcggctggc caggttaccg aggccgttgg caagatccga gttgtagcgg ccgacgagcg
    19861 cgtcgtaact gaaattgccg tcctgcccga agacgatctc gcgaagcagg aagtagcgga
    19921 ggcagtcgac accggcgact tccttgattg gcgtcgcgcg cacaatgttg ccgcgcgact
    19981 tgctcatctt gtcctgctcg aacagcagcc agccgtgcgc gaagatccgc ttcggcaacg
    20041 gcaacccggc ggccatcagg aacgccggcc agtagacggc gtgaaagcgc agtatctctt
    20101 tgccgatcag gtgcaggtcc gcaggccagc ggtcttcacc ctccacagcg ctcatgtaag
    20161 tcgaaagcgc gtcgaaccac acatagaaca cgtggtttcc ctcggctgga acagggatgc
    20221 cccacttgat cgtcgtacgg ctgatcgaca ggtcgctcag gcccttgctc acgaacgaga
    20281 gaacttcgtt gcggcggctc tccgggcgga taaaatccgg ctgttcctcg tagagcttca
    20341 tcaacggctc ctggaaagcg gaaagcttaa agaaatagtt ctcttccgtg accgtttcgg
    20401 tcgggcggcc acaatccggg caggggtcgc ccggcttggc gtcggagaca tagagctcgt
    20461 cgaagacaca gtactgtccc gtgtacgagc ccttatagac gaagccgttt tccaagcatc
    20521 gacggaacag gtcctggaca accttgtgat gttgcgggct ggacgttctc tggaagcggt
    20581 caatcgccag tccgagcgtc tcccactgcg tgcggaactc ggcggagatg agatctgtga
    20641 actcctgcgg attcttgcct gtcgccttcg cggctcgctc gaccttctgg ccgtgttcgt
    20701 cggtcccggt ggccagcacc gcgtcgtagc cctgcatccg tttgaggcgc cggatggtgt
    20761 cagccgcgat ggtcgtgtag gcgtgaccga tgtgcggggc cgcgttcaca tagtagatcg
    20821 gtgtggtcag gtagtatttc ggtttcattg ctgggttctc agataggcca aattcgcttc
    20881 cgcgaggacc tgtttcagcg gcacgccggc ttcgcgcgcc agcatccggc aatcctcata
    20941 ctcgggcgca taggcgccgg attcgccgac tttgacgcgc actttgccgt aagacgtttc
    21001 gacttcaacc atccgccgtg actgaacacg gcgttcggcg gcgaagatgc ggacgccaag
    21061 cgtggtggtt tcggcaaaca ggatttggga caaccgctcc cggtcttccg ggcgggcgat
    21121 cacacggatc aacgttcccg gacgattctt cttcatgaag acggggcaga acgacacgtc
    21181 gagggcgccg ctctccaaca gacgctccaa cgtgtagcca agcacctgcg gagtcacgtc
    21241 gtcgacattc gcctcgatca ccgagaccgt ggccgcctcg acggcgccgg tcggctcgcc
    21301 gatcagcacg cgaagcacgt ttgcctgctc cttgaagtcg cgcacgccgg cgccgtagcc
    21361 gatagccgag attctcatcg ggggcagggg ggtgaaactc tccgacagcg tcaccgcgat
    21421 cgccgctccg gtgggcgtgg tcagctccac cgaggggccc cgcgcataga ccggcttgcc
    21481 ggcgagcaga ccggcggtcg cgggcgccgg aaccgggagg gttccgtggt ccgtgtccac
    21541 cgtgccgctg ccgagattca ccggcgagca gtacacagtt tcgacgccga gcatgtgaat
    21601 gcccagacag gcccccacga tgtccgcgat ggagtcgatt gccccgactt cgtggaagtg
    21661 caccttctcg atcgggacct gatgaaccgc ggcctctacc tcgccgagac gccggaagac
    21721 ggtggaagag tcacgcttga cgcggtcagg cagtgccgac tgctcgatca tgccgagaat
    21781 gtgcggaagg tgacgatgcc tcttgttgtc gccgccgctc acatggaatt tggtcgagcc
    21841 gatcccgcac cgcacggtct tctcgaagcg gatctccgca cccggcgcga gcgaagacag
    21901 ggcctcgacc agggtagggg cgtccgcacc cgcgtcggcc agagcgccga ccaacatatc
    21961 gccgctgatc ccggagaatg cgtccaggta acagatcttc atcgaatcaa ctccggcaaa
    22021 atctccccgg cctttcccct caatgagcag tcggcctggg ccgaaaacgc cgtctcctcg
    22081 aggtttacct cgatcactcg cgctccggcc cttttggcga gtggtgtcag gccggcagcg
    22141 ggatacacga cagaggacgt cccgaccacc aggcagacat ccgccgaccg cgcggctctt
    22201 tccgcgtcgt tccacacatt catcggcagc gcctcaccga accagacgac acccggcctc
    22261 aacatgcccc cgcaatcgca gcgcggcggc agctccggca gcggtgttcg ctcgtcccgg
    22321 tgctcaacac cgcacgtggt gcagcgcaga tgccagatgt cgccgtgcag tttcagcaca
    22381 ttccggctgc ccgcccgatc gtgcaggccg tccacgttct gcgtgacaag cacaaaagaa
    22441 gacttcttct tttccagccc ggcaagggag taatgaccgg cattcggcgc cgccttggcg
    22501 atcagtccgc gccgccagtc ataccactcc cacaagactt tcggatcccg agcgaacgcc
    22561 tcaggcgttg caagatcctc gggccggtac tgccgccaga ggccgctgtt tcctcgaaac
    22621 gttggaatcc cgctctccgc cgagatgccg gcccccgtga gcgccaccac cgcggacgcg
    22681 gcctccagcc actcacgagc ctgttgcagg gactccacgt atctatcgta ccggaacagg
    22741 tgcttcggac tgcgctcaag ggagtttttc cgccagcact ttcttcgcct gcatctgccg
    22801 gaactcccgg agtttctccc gcaatccagg acgcgaattt gccaggatgg ccacggcgag
    22861 cagcgcggcg ttcaccgccc cgggcttgcc gatcgcgagc gttccaaccg gaatgccgcc
    22921 cggcatctgc accatcgcca gaagggagtc caaccccttc agcgactggc tttcgatcgg
    22981 gactccgaga accggaacaa gcgtgtgagc ggcgacgacg ccggcgaggt gggccgctcc
    23041 gccggccgcc gcaatgatca cttccacgcc tcgcgcatca gcctgcgcgg catattcagc
    23101 cgtctccgtg ggcgttcggt gcgcggacag cacccggcac tcatggggca cctcgaaacg
    23161 gtcgagtgtc tccgatgtct gccgcatcgt ctcccagtcc gacttgctgc ccatgatgac
    23221 ggcaaccagg ggtttcgtgt cgttggtcat ggtatccgga ataccctaag ttaatccgtc
    23281 cgaaggacag tgggcaaccc gagtgtgcca gattctgttc ccgatgtgat atttcctggt
    23341 aaaataaagg ctgcgccggg gcaacctgag gcggccccat cgcaacccgg ctttctggga
    23401 ttcggtctgg ctggaaaact tgcagcgcta cagcaatttg agcaacctca tgagggacta
    23461 ttttgcgctc acaaaaccgc gcatcacgtg gctcattttg atgagcacgg gcgtaggcta
    23521 cttctttggg ctgcgtggcg aatgggacct gctcacgctg ctgcacacga ttctgggaac
    23581 aggtttgctg gcttccggca ccgcgacgct gaaccagtgg tacgagcgcg acgaagacgc
    23641 gaagatgcac cgcaccgaac ggcgtcccat cccagcgggg agagtgacac cggccaaggc
    23701 ggcgattttc ggcatccttc tctcagccgc cgggtttctg gaactgtccc ttgcggtcaa
    23761 tctgctgacc ggcctgcttg ggctgattac gctagccagc tacctcctcc tctacacgcc
    23821 gcttaagaag aggtcttcgc tttcgacgac cgtaggcgct ttcccgggcg ccatgccgcc
    23881 actgatcggt tttgcggccg ccagcggcac tcttaccgcc caagcgtggg tcttgtacgt
    23941 catcctcttt ctgtggcagt ttccgcactt ctactcgatc gcgtggatgt atcgcgaaga
    24001 ctacggccgc gccggcatcc tcatgctgcc ggtagtggaa cccgacggcc gctcgacgtc
    24061 gcgagagatc gtcctttatt cgctggcgct gatcccagcg agtctgctgc cggcgcttct
    24121 ttcgatgagc ggcagaatct acctatgcgg cgcgctcgtg ttgagctcgt tttacttcta
    24181 cacaggtctg cgcgtccttc ttgagcgtac ggccctgcgc gcgcgaaagg tcctgctggc
    24241 ctcggtcttg tacctgccgc tgctttacgg tctaatgctg ctggataggc ctggtctgtg
    24301 atcgtctccg tcgagaatct gactcataac tacgccgcaa ggaccgccct ggcgggcgtc
    24361 agctttgccg tcaaagatcg cgaggtcttc ggcctgctag gaccgaacgg cagtgggaag
    24421 tcgacgctgt tccggatact ctcgacgatg atggctccgg cgtccggccg ggtcacagtg
    24481 gctggactcg acgtgggcag tcggccggcg gaggtgcgcc gccgcatcgg tgtggtcttc
    24541 caatcgcaga gtctcgaccg aaagctgacg gtggaggaaa accttttcgg ccagggccat
    24601 ctgcacagcc tctccggggt aacgctccgt gagcgagtgg acgaagtgac ggcccgtctc
    24661 ggactggaag accggcgcga cgagatcgtc gaaaaactct ccggcgggct gaagcggcgc
    24721 gtcgaaattg cgaaggccct gttgcacaaa cccgaagtgc tgctcatgga cgagccttcc
    24781 accggtctcg atccgggggc acgacgcgag ttgtggcaat acatcgaaga ccttcgggcg
    24841 aatcaaggcg tcacagtgct gctgacgacg cacattctcg acgaggcgga tcggtgcgac
    24901 cggctgctgc tgctgcatca ggggcgcatc gtggcggaag gagccccggc ggaattgaag
    24961 tcgaggattg ggggagacgt ggtcgtgctt gaggttgccg acctcgatgg gatgcaacgg
    25021 caactggccg aacggtttca caccgagccg cgcgttgtcg atggcgccct tcgtgtcgag
    25081 attgcgaacg gtcaccgctt tatcaccgaa gcggtcgaag cgttcccggg tgcgatttct
    25141 tccgtagcgc tacacaagcc gacactcgaa gacgtcttcc tggacgagac gggagttgcc
    25201 atttgctagg aaagtctttc tggttgcctg tggccacgtt gtggcaaagg gagctggttc
    25261 gcttctggcg cgaaaaggcg cgcgtccttg gctttgttgg ttcaccgctg atcttctggc
    25321 tggtaatcgg ctccggcttt ggcaacctgg gctttttctt tcccggcgct ctgacactga
    25381 ccgtgatgtt ctccgcggtc ttttcgacga tgtccctgat tgaagaccgg cgggaggggt
    25441 tcctgctttc gatgctggtc tcgccggcgc ctcgcagttc gatggtactc ggcaagactc
    25501 tcggttcggc gactttggcc tggattcaag gaatgatctt tcttgctttt ggaccgttgg
    25561 cgggagtccc gttatctttc gacgttttgt ggcagtcggc ctcagtgatg ttcctcatcg
    25621 cgatgacgtt cacgctgctc ggctttgcgc tggcctggcg gatggagtcg tcgcagggct
    25681 tccacgcaat catcaacctt cttctgttgc cgctgtggat ggtttccggc tcgctctttt
    25741 cgatggcgaa agcgcacggc tggatcagag tcgtcatggt gctgaacccg atgacgtacg
    25801 cactggcggc actgcgaagg gttctgggga cccaggttag tgccggtacg ccggaactgg
    25861 gagtgagctt aacggtgacg acggtttgcg ccgtcgtatt tttgacggtc tcggccctgg
    25921 tcgccggcca aaagaccgca aggaactttg catgagatcc cgagctaccg tgccagcaac
    25981 gatccttgcc gggctcggtt tcctctcgct ttcaggctgc gcgccgaagg ctgagccgct
    26041 acccgtcctg ggagaaattc ccgagttcac cctcaccgca caggatggcg cagaatttca
    26101 tggcgggtcg ctgcgcggga gtgtctgggt cgccgacttc atcttcacga cctgcgccgg
    26161 tccgtgcccg cgcatgagct ggcaaatgag caggcttcag gaagcgttct ccgacaagcc
    26221 ggacgtgaag ttcgtttcgt tcaccgtcga tccggagcgg gacacgcccg aagtgctggc
    26281 ggcctacgga aagcgctacc aggcgcaggc gggacgctgg tacttcctga ccggccctga
    26341 gcaggccctg cacaaactga acagggaagc attcaagttg ggcaacgtgg acggcagtct
    26401 caaccacagc actcgatttg tcctgctcga ccggcagtca cgcgtcaggg gctattatga
    26461 ctcctcagac gccgactcca tgaagcaatt gctcgcggac ctcaagaatc ttgtcaggga
    26521 gcggtcttga tcgaactgcg cgaccttccg gccgtgaacg cgcttctgaa cctcacggcg
    26581 gcgtgtctcc tggtttgcgg ctacgtgctg atccggcgcc gccgcatcca ggcccataag
    26641 cgcgtgatgc tcgccgcgtt ctcggtgtcg gtccttttcc ttatcagcta tctcgtctat
    26701 cactaccagg tgggttccgt ccgctttcag agaaccggaa cgattcgcac gatctacctc
    26761 ggcatcttgt tcacgcacac ggtattggcg gcggtcgtgc cggtcctcgc ggttgtgacg
    26821 ctcttccgcg cgctgcaaga gcgtttcgac cgtcaccgga ggcttgcccg ttggacgctc
    26881 ccgatctggc tctatgtgag cgttacgggc gtcacggtgt acttgatgct gtacaggctg
    26941 tagcagacct gcttcgctag ctttcccgcc tccgtccgga cgatataatc acgcgacgag
    27001 gaggtgtccc aagtgagcac agcacttcgg tggagcattg catttgcgtc gttgggtgtc
    27061 gtgggcgccg tcgctgctac tacgccaaaa gtcatcccag caaggcacaa ggtgcggatc
    27121 gaacgcgccg tcatgatccc gatgcgcgac ggcaagcgtc tgtcggcgga cctgatccgt
    27181 cccgacagcg aaggtcgctt tcccgtggtg atgatgtatc agccctatcg caaggacgac
    27241 gtgggccgcg gcggagtcgg tccgcaccag tactttgcgg agcgcggctt cgtcgccgta
    27301 cgtctggatg cccgcgggac cggcacctcg gagggtgtaa ataccgacga gtacaccctt
    27361 caggagcagc aggacggcta tgacgccgtg gaatggctcg cccggcagtc ctggtcgaac
    27421 ggcaacgtcg gcgtctgggg tatttcgtac agcggtttca cggcgcttca ggtggcgacg
    27481 catcaaccgc ctcatctgaa ggcgatcgtg ccgatctacg ccaccgacga ccgctacacc
    27541 gacgactgcc actatgaccg cggcggcaac atgcgcatgt attacgacgt cggacactac
    27601 ggtggttgga tggtggcaat gaacgccctg ccgccaatgc ccgagcttgc cgggcctcaa
    27661 tgggctgaaa tgtggaccga gcacctccaa aacaacgagc cttacctgct caagtggatg
    27721 aaacagcaga ccgacggccc gtactggcgc aacggatcgc tccggcccgg ctatgagcgc
    27781 atcaagtgcc cgacgttcct gatcgccggc tggcgcgacg gctatccgaa cgcgatggtg
    27841 cgaacctaca tgaacctgaa ggtccccagg aaattgctgg tcggcccgtg ggttcactcg
    27901 ctaccaaaca gttccgtgcc cggcccccgg atcgactgga tgaatgaggc ggggcggttt
    27961 ttcgcccact ggctccgcga cgaggatacg ggaatcatga ccgagccgcc agtgacggtc
    28021 tacatgcagg agtacagcgt gcctgatcgc atgctcgacg taacgcccgg ttcctggcgc
    28081 aacgaagcgg acttcccggc ttccggcaca agcgaaatca cgttctatat cgatgaaggc
    28141 ggcaagctcg cgtccactgt gggggctgca ccccgccgcg atcatgacga attcgaatat
    28201 gtcccgacag tcggcctctc gaacggttac tggtccggcg gcgggatacc gttctacctc
    28261 ccggacgacc agcgcggcga cgaggcgtat tcgctggtct acacgaccga gccgctcgac
    28321 cgggagctcc ggattctcgg ctggccaaag gttattctgc acgcctcgtc gtccgtgcgg
    28381 gttgctacgt ttgtcgcgaa acttgccgac gtggccccgg acggccattc ggcgctcatc
    28441 gtcgacgggg cgctgaacgg aacccgccgg cagtcgctta cgactcccag cgcgatgtcg
    28501 cccggtgaag tctacgaact cgcaattccg atgctgccca ccggatgggt gctgaaaccg
    28561 gggcatcgcc tccggctggc ggtttccagt tccgatttcc ccaacctctg gcccacaccg
    28621 gaacctgcac ggaaccgcgt gttccgcgga gggcgctatt cctcgcgcat cgtacttccc
    28681 gttgtgccgg agtccaaact cccggcgccc gagtttctgc cacctccggc cttgcgtcag
    28741 atcgtctccg gaagcggatc gccgccggtg atgcacgccc tctatgacca gattgccggc
    28801 acggtcactg tgctcggaca acggaagggc gccaccatcc tggatgaggg tctcgggacc
    28861 gttctgtcag acagtgagtt ccggtgtacc gcgtcctccc gcgatcccgg ccgcgccagc
    28921 atcatcggca cacacaagat cgcgtttcag cgcgaggacg gattgatcga agtcactgga
    28981 gaaagctcca tccgcgccac gacgacccac ttccacattc tggtgaacct caccgtgacg
    29041 cggaacggga tgcctttctt ccagaagcag tgggtcgcga ccgaaccgcg caacctgctg
    29101 tgagtagcgg agtacgatgt gcgaattcag gaggagaagt tgaagcgact acagccagtg
    29161 ctgaaccgcc gccaattcgt tgctggcggg gtggcggcgt cagggatgtc ctgcgtcgcc
    29221 gcggggtcga gtgtaccccc gaacatcgtc atcattctgg ccgatgacct tggatacggg
    29281 gacctgagct gctacggcca tccgacgatt caaactccga acatcgaccg gatggagcgt
    29341 gaaggaatgc ggttcacgca gttctactcc gcagcgtcgt tgtgcacgcc gagccgcgcc
    29401 ggattaatga ccgggcgcct gccgatccgc tccggtctga cacgcgtcct ctttccatgg
    29461 tcggagggag gcatccagga ttccgagatc accatcgctc gcttgctgaa gaaatccggc
    29521 tacgccgcga tgtgcgtcgg aaagtggcat ctcggacacc atcgccggta tctgccgatg
    29581 cgtcatggtt tcgacggcta ctttggaatt ccttattcca acgacatgag caaggcgacc
    29641 cagcccacgg catcctgggt cgatcggacg ccgccaacgc cgctgattcg tggggaagaa
    29701 gtgatcgagc aggaacccga ccagcgctac ctgacaaggc gctataccga ggaggcgatc
    29761 cgcttcattc gggagtcctg ccgcgcaggg cggccgtttt tcctctattt gccgcatacg
    29821 tttccccaca tgccgttatt tgcgagtgaa cgttttcgcg gcaagagcct ccgcgggctt
    29881 tatggggatg ttgtcgagga gctcgactgg agcgtgggcg agattctggc cgaactcggc
    29941 cgtctgggtg tcgacggttc aacgctggtc gccttcacta gcgacaacgg gccagccacc
    30001 ggctcggcgg gactgctgcg ggatcagaaa gccagcactt tcgaaggagg catgcgcgtc
    30061 cccttcatcg cgcggtggcc tggaagaatt cccgccgccg tgacgacgtc ggccttcggc
    30121 acgacaatgg acctgtttcc gacttgcgcg aggctcgccg gagcgacgct gccgactgac
    30181 cggatatacg atggcgacga tctgacgccg gtgctactta gaaactcgac cggccgcgag
    30241 ccattgttct tctattactc aaatctcgat gacgatcagg agctgaaagc cgtgcgcaag
    30301 ggacggtgga agctccacac gtccatcggc tcgttccgaa aggcgccttt gttgccgaat
    30361 gaatccgggc cgccgctgct tttcgatctc aacgaagatc cctccgagca gcggaatctt
    30421 gccggcacaa agcctgaaat cgccaaggaa cttcgggtgg tgatggaagc acacaaagcg
    30481 agcgtaagac cggggccgca gcaactgtga acttagctta gcagttctca ctcgccgatg
    30541 atcttcacca ggacacgctt gcggcgccgg ccgtcgaatt cgccatagaa gatctgctcc
    30601 cagggaccga aatcgagcct gcctttcgtg accgccacca cgacctcgcg ccccatgatc
    30661 tgccgcttca tgtgggcgtc ggcgttgtcc tcgccggtgt cgttgtgccg gtactgcgag
    30721 accggctcgt gcggtgcgag attctccagc cacttgtcgt agtcgtggtg caaaccggat
    30781 tcgtcgtcgt tgatgaagac cgacgccgtg atgtgcattg cgttcaccag caccagacct
    30841 tcctgaacgc cgctctccgc cagacacttc tccacctgcg aagtgatgtt gaggaaggcc
    30901 ctccggcccg gcacgtcgaa ccacaactcc ttgcgataac tcttcatttg ccctggactc
    30961 cttgcaactt tccagtatcc taccccggcg ccctttcaaa gcgccgtaat ccggtgtcaa
    31021 atagtgttga gacatattga ttctttcgcg gagagaaccg ccagtggcaa tcgatcattc
    31081 cttgaagacc cccaaaaagg cgcgcgcggg cgtaccagtt gcggccatta tcattcttgc
    31141 gatggtcgtc atcggggccg ccggattctg gtacctggac cggcagtcca agtccatgcc
    31201 aaaagatatt ccggtattga cggcagaagc caaggcctat gtccgtaacc tgaagctcgg
    31261 ggaagtcgaa atgaaggcgg ccgaggcttt cgcaggccag accctcgtgg agattctcgg
    31321 gaaaatcacc aatgccggcg accgcaggct ccagtccgtc gaaatcaact gcatcttcta
    31381 tgacccctat gggcaggtgg tgcttcggca gcgtgtcccg atcgtccgga gccggagtgg
    31441 cggcctcgcc cctggtgaaa cgaagtcctt tcggctcccg ttcgatgaga ttccggatag
    31501 ctggaatcag gccatgccgc agcttgtaat cgcggaaatc aactttcagt agaggcggag
    31561 ttggcactga tcctcctagt cgatgacgat cccgatcagc tcgagatccg ccggctattg
    31621 ctcgagacca gcggtttcga ggtgcggacc gcttcgacaa ccggggaggc cgagcggctg
    31681 ttcggtgagt gccctcccgc gctggtcgtc acggatctgc ggctgcccac cccttccgac
    31741 ggaatccgac tgatgaggat gatgcgggct cgttccgccg gcgtgccgat ccttgtgctc
    31801 tccgggatga catcggatct cgcccacgtc gaggatgggc tcgccgacga agtactgcaa
    31861 aaacccttcc gggctgatcg tttccttgaa ctcgtgcatg agtacgcgca tggacctcgc
    31921 tgagctgcgc cgttccctgg aacttgggcg ggaggtccgc ctgaaggtga aagtcgtccc
    31981 gaaaagctcc cgctcagaga tcgccggttt tatgggagac gggacgctga agctcaaagt
    32041 ccaagctccg ccggaacgag gaaaagccaa cgcgcaggtc tgttcggtac tcgccgcggc
    32101 cttcggcgtg cccgagagga acgtgtcgat cgtaagcggc gagacgtctc cgctcaagca
    32161 ggtcgttgtg gccccgccct ccgacttgcg atgccggcgt gccccgggat agagtggggc
    32221 atttccagga ggccccatat gcccgctgcc tgtttccagg actcttcgac tcgccggtcc
    32281 gtcatctcca gtctgttcgc agccgccggc tggtccgctt tcgatcgcgc ggaagccgcc
    32341 gtccggcaat cggccggcag agacgctccg atcaccggga aggacaagct caggattacg
    32401 aaactcgaga ctttcctcgt caagccgcga tggctgtttc tcaaggttca caccaacgcc
    32461 ggaatcgttg ggctcggcga accgatcacc gaaggccggg cgctgacctg cgctacggcc
    32521 gtgaaggaga tcgaaccgta cctcgtcggc aaagacccga ggcaggtcgt tcatcactgg
    32581 caggccatct accggcacgc gttttaccgg ggcgggccga ttctgaccag cgcgctgagc
    32641 gggatcgatc aggcgctctg ggacatcaag ggcaaggcgc tcggcgtgcc ggtttatgaa
    32701 ctcctcggcg gacccacccg tacccgtatt cgcgtgtacg cccacgccgg ttcgccggaa
    32761 agcatcaagg aagccatggc acgcggcttc accgccttca aaaccggacc ggctaagaaa
    32821 cgtcccgcgc ggtatgtcga gacacccgcc gcggttcagt atgccgtcga acagttcgcc
    32881 gagctccggc agcttgtcgg aaaagaggcg gacatcggca tcgacttcca cggcgctatc
    32941 agcccggcgc tggctaagct tctgatcaag ggcctggaac cctaccagcc gatgtttgtc
    33001 gaggagccat gccaggccca gaaccacgat gtcatggccg agatcgcccg tggaacgcac
    33061 ctgcccatcg ccaccggcga gcgcgttttc accaagtggg gcttccgcga ggtgctggag
    33121 aagggcgccg cctccatcct ccagcccgac ctctgccacg ccgggggcat caccgaatgc
    33181 cgcctgatcg ccggaatggc cgaggcctac tatgcctcca tcgcgccgca caatcccctg
    33241 ggcccgattt cgctggcggc aggggtgcag ctctccgcct ccatcccgaa cttcctgtgc
    33301 caggagcagg tcagcctcgg agagggctac atcaagaaac cgttcacggt ccgcgaaggc
    33361 tacctggact tgccagatgg cccgggcctc ggaatcgaac tggacgagaa cgcgatggcg
    33421 gacaagatcg gccacgactg gcgcaacccg gagacctacg acgaagacga cggctcggtg
    33481 gtggactggt aagatcgaag agaaatgacg aagcagacca tcctgtcctc tattattgac
    33541 atcggcatcg ttccggtcgt ccgcacctcg agcgcggaaa gcgccatcaa ggccatcgaa
    33601 gccatctaca agggaggcat ccgcgcagcc gagatcacga tgaccgttcc gggcgccctc
    33661 cgcgcgctgg aaaaagtagc cgacaagttc ggcgacaaga ttgttctcgg cgccggcacg
    33721 gtccttgacc cggaaacggc tcgcgcctgc atgctcgcag gggcggagtt ctttgtgacg
    33781 ccggctctga aaccggccac tatcgaaatg gcccggcgct actcgaaggt catcatgccc
    33841 ggtgcgctga ctccgaccga agtactcaca gcatgggagg ccggcgccga catcgtgaag
    33901 gttttcccct gcggcaacgt tggcgggccg aagtacatca aggccctcaa ggcgcccttt
    33961 ccgcagattg agatggttcc gacgggcggg gtcaacctgg aaaccgcggg tgactttctg
    34021 aaggccggtg cctgcgctgt cgcggttggc ggcgaacttg tggacgccaa gactatcaag
    34081 gaaggccgct acgaagtctt cgaagagcgc gccaggcagt acctcgccgt tgtcgccaaa
    34141 gcacgcgagg agatgaagtc cgccaagtga acttggtaaa ctgagaggcc ggttatggcg
    34201 atcagcaagg atttgctgga gattctcgtg tgcccgctct gcaaggcgcc ggtggaactc
    34261 aagcaggatg agaatggcct gaaatgcgtc gaatgcaagc gggtataccc aatccgtgac
    34321 gatatccccg tgatgctggt cgacgaggcc aggatcgaag agtaagcccg ttcctcggat
    34381 gccagacgcg cttcagcgct tgccgggcgg cgcgcgggtg gcggtcgttc gcctgcgctc
    34441 actcggcgat tgcgtcttga ccacgcccgc cctcgaaatt ctcaaacagt tccggccgga
    34501 tctcaggaca gcggtcgtcg tcgaggaccg gtttgcgcct gtgttcgagg gaaatcccga
    34561 cgtgaacgcg atattgccgc cggccgcctc cgccctccgc ggctggaaac ccgacctgtg
    34621 catcaacctc catggcggca tgaggagcgc cgtgttgaca gcggcatcgg gcgcacgctt
    34681 tcgcgcaggt ttcgctcact ttcgggcttc gcggctctat aacttgcgca taccgacggc
    34741 gcagacgata ctttctgtgg accgcaaggt ccatacggcc gagcacctcg cttccgccat
    34801 cttctggctg ggcgtaccgc gctgcgagat cccgtgggca cggctcttcg ctcatgacgc
    34861 cggccttagc ttgcgtggct cctacgctgt cctccatccc ttcgccactg gtccgggtaa
    34921 ggcctggccg gcttccggtt ttctcgccat cgcgtcccac ttgcggttgg cgctcgggtt
    34981 agatccagtt ttcatcgccg gacctggcga cgacacgcac ccgttcgcgg aatatcgttg
    35041 catcgaggga gcgccgctgg cggacgtcaa gtcgttgctt cggggcgcca cgctgttcgt
    35101 cggaaacgac tcgggtcctg cgcacatggc tgccgcgttt gggttaccgg tcctcgtcat
    35161 tttcggcgcc tcagaccccg tgatctgggc tccgtggcgg acggtttccc aggtcatcgt
    35221 ggctcgcgga ccgatcgaag cggtcgcggt aggtgaagta atcgaagcgc tcgagcgtct
    35281 gagggtcgcc gcatgaagga atggctgcgg ctgctcggct tcgtccgccc ctatgtgctc
    35341 gtgctggtgg tgtcggtcgt cttgatggcc tgcgtcggcg gggcccacgc tttgatcgcg
    35401 ctgctgatcg gcccggtctt tgaccgtgtg ctgaatccgg cctccgcgga cgcgccagtt
    35461 ctactgttcc ggctgccgat cttcggtcag gcggtctatc tgaacgacct cctgccctcg
    35521 tggatccaca acgtgtggac catgatcgcg ctgggcatct tggccgtatt tctcgtgcgc
    35581 ggcctctgtg actacttcgg caactacttg gtcaactacg tcgggtattc ggcagtcacc
    35641 gacttgcgcc agactgtgtt cgacaagatt cttcgccaga gcgcggagtt cttcgagtcc
    35701 cattccaccg gacggttgat gtcgtcgatc atgaacgaca tcgagaagat ccaggttgcc
    35761 acgtcgcaca tcctggcaga cttaatgcgg cagattttca tcgtggtggg tcttctctat
    35821 gtgctgctcg aaaaagactg gaagctggcc ctcgtcagcc tcacgctgct gcctttcgtg
    35881 ctcgtcccca cagcccggat cggaaagcgc atccggcgca ccacccgccg tgcgcaggac
    35941 gacacggccg atctgaacca gatccttcag gagaccctga gcggccacca ggtggtacgc
    36001 tcgttcggcg cggaggccta cgagtcgcgc cgtttccggg tcgtcgcgcg gcgtctccgc
    36061 tccagcaatc ttcgctatgt agcgcagcag gcactcagct ctccgctgat cgagttcttc
    36121 ggcgctctca ccatcgtcgg cctgcttacg tacgcccgaa cgcagatcaa ggcaggccag
    36181 atgaccaccg gggagttcac gactttcgtc gtcgctctgc tgatgcttta cgagcccgtc
    36241 aagcggctca cggggattca caatatcttc cagcaggcgc tcggggcttc ccagcgcgtc
    36301 ttcgagtatc tggaccgtga ggaagcaatc caggacaagg ccggcgcgtt gccgctagct
    36361 cgcttcgaaa gatcgattgt ctttgacgcc gtgagcttcc gctatccgag ttctcccaac
    36421 ggctttctgc tgcgcgacat cagcctcgag gtgaaagccg gcgatgtcgt cgcggtggtc
    36481 ggcccaagcg gcgccggcaa gagcacgctt gtcagcctcc ttccgcggtt ttacgacgtg
    36541 accgcgggcg cggttcgcgt agatggtcac gacgtccggg acctccaggt ggcttccctc
    36601 cgacggatgg tcgggatcgt cccccaggac accttcctct tcaacgacac ggtggcgaac
    36661 aacatccggt acggccgcca ggacgtatcc gttcgggagg tcgaggaggc agcgcggaac
    36721 gcgctttgcg aggaattcat cgggcagatg ccggagggcc ttgacaccgt catcggagag
    36781 cgcggcttca agcttagcgg gggccagcgg cagcgcatcg ccatcgcccg cgccctactc
    36841 gaaaacgctc ccattctcat ccttgacgag gcgacttccc acctcgatac cgagtccgaa
    36901 ttattggtgc agcgcgcgtt ggggaacctg atgtccgggc gcacggtgat cgtgatcgcc
    36961 caccggctgt cgaccatccg ccgcgcggac aagatcatcg tactggacca gggccgtatc
    37021 tgcgaggtgg gcacgcacga cgaactggtc ggttccggcg gcatctatca gcgtttatat
    37081 gaacttcagt tcctggaggc agaccgtctg gcctagctat gaacgttcgc agcatgaccg
    37141 gcttcgcccg ggtccaaaaa caaaacgatg tgggggaggc cgcggtcagc atcaagagcg
    37201 tgaaccaccg tgcgctcgac ttgcactttc gcatgcccgg cgagcttgac ccgttcgagc
    37261 cggtgctccg tgcggccgtt aagcggcaca tcgttcgcgg ccacattcaa attcaggtca
    37321 cgtttttccg ctcctccgaa gcccctcccg cggccctgaa tcgctctgtg ctcgatgcgt
    37381 atatctcggc ttaccgccag gctgccgcgg agcacggact cggaggtgaa cccgatctga
    37441 acgctgcgct ttcgctcccc ggcgtttttc gggaagcgcc cgacgaagaa ccagatcgga
    37501 ccgtcgagaa gcttcttctc gaagctgcgg aagaggctct ccaggcgttg aatgcctttc
    37561 gagaacgcga agggggcgag atcgtgggcg aattgctcga cagggcggcg gcgatccgag
    37621 attgcacgtc gcgcatagag gagatccgtt cccgtgcctt gccagccttc caggcacggc
    37681 tcacggagcg gctggcggag ctgcttcaga acgtgcccat tgatacccag cgcctggctc
    37741 aggaagcagc catcctcgcc gaccgcagcg acatcggcga ggagctaacc cggctccgga
    37801 tccacgccgt acaggtggaa gatctgcttc ggaaaggggg agaggtcggc aagaaactcg
    37861 acttcctcct ccaggagatg aaccgggagt cgaatacgat cctgtccaag tccaacggga
    37921 tcggagagca gggtctggag atcacccaac ttgctctggc cgccagggcc gacattgaga
    37981 aaatccgaga gcaatccctc aatctagaat gagcatcgta ttcatcgttt cagcgccgtc
    38041 cggctcgggc aagtccacgc ttgtgggccg acttctgaag gacgtgccaa ggcttttatt
    38101 ttctgtatct tacacgacca gagcgccccg cggaaacgag cgccaggacg aagactacca
    38161 cttcatttct cgtgccgagt tcgaggagcg gatcgcccgg gacgagtttc tggagtgggc
    38221 agaggtattc gggaactatt acgggaccca ccgcgagatc ctggaccggg cgcggtcgga
    38281 agggtccgat gtggtactcg acatcgacgt ccagggtgcg cgacaattga agagacggat
    38341 cctggatgcc gtcagtatct ttattcttgc gccttcccgc gagatcctgg agcaacggtt
    38401 gcacgaccgc tcagaggacc cgcccgaggt catcgccaga cggttacgcg aggcggcgga
    38461 ggagattcgt aactgccgcg cgtacgacta cgtcctcgtc aatgacgaag tggaacggtc
    38521 ggctgatacg ctggaggcca ttgtgaaggc cgagcgcgcc cggcgtacac gcatcgaaga
    38581 gaagatccgg ccaatcctgg ccaccttcga ggcgaagtaa gcagaggttt tcgagcaatt
    38641 atgtctgaca ggctgtcacg aaacgcgagt ttcatcatcc cggacgaccc ggagcagagt
    38701 acgtatcgct ggatcatcgt cgccgcgaaa cgcgcgcggc aactccaggg cggagcccgg
    38761 ccgttccttc caaccaccgc caagaaaccg accgtcaccg ccatggagga gacgcgtcga
    38821 ggtctcgtga agtgggaaga cttggctctt cagccgcagg aagagcaaga acaagaacag
    38881 gtttgagcca atgaaggttg tgctcggagt cgggggcggc atagcggcct acaaagcggc
    38941 agagctcgtg cgcgccctgt ccgagcgcga cgccgaggtg caggtcgtca tgacggaggc
    39001 ggcccaggag ttcgtccggc cgctcacctt cgtggctctg agcgggcgca aggtcatcac
    39061 cagcatgttc tcctccggcg gcgccgagga aacgctttcc agcgccgtcg agcacatcca
    39121 ggtcgcgcag gaaaacgata tcctggtggt cgcgccggcc actgccgatc ttctggctcg
    39181 cttcgcccac ggtatcgcca acgatttcct tacaaccttc tacctggcgt tcaccggtcc
    39241 ggttgttctg gcgccggcga tgaacaccaa catgtggaac cacccggcca cacaagcgaa
    39301 cattggggta ctccgctcac gtggacatag catcgtcgga ccggaagacg ggtttctcgc
    39361 ctgtgggatg gtcggccccg gccggatgtc ggaaccggca gaaatcgccg aagccgtgct
    39421 ctctctgtct cggcgcaggg acctggaggg cgagaccatt ttggttaccg ccggtcccac
    39481 gcaggaaccg ctcgatccag tccggtttgt ttcgaaccgg tcgtccggca agatgggtta
    39541 cgccctggcg caggccgccg ccgaccgggg cgcgcgcgtt gttctcattt caggaccggt
    39601 gcacatcccc gaaccgtccg gcgtgcagat cgttcacgtt cgaaccgcga ccgagatgcg
    39661 ggatgcggtc ttggccgggc tcgacgagtc caccatcatc gtgaagtccg ccgccgttgc
    39721 cgactaccac gtctccacgg tgccgcaaca gaaaatgaag aagacggctg cgcggatttc
    39781 gctcgaactc gatccgacgc cggatatcct ggccgaattg ggtcagaaga agggcgaccg
    39841 gctgctcatc ggcttcgcgg ccgagaccga gaacctgcgg caggaggtgc ggcggaagct
    39901 cgcgtcgaag aactgcgaca tgcttgtggg caacctcgtc ggccaggaag gcgtgggctt
    39961 cgaatccgac accaatgaag ttctgctcgg ccttgctacc ggcgcgctga tctcgattcc
    40021 gaaggcttcc aagcgcgagg tcgcggatcg catcttcgac gaggcgctga aattgcgctt
    40081 ggccctccac gccgcgcaat gacccgggag ttgctccgcc agtacctctc gttctatcag
    40141 gatctcggca ttcggactct ctaccgccgc gagccccacg ccgcaccacc gtctgcgagc
    40201 cgggaaccgg ctccgctcga tctgctgtta cagcctcagg cggaaccgga agcggttcag
    40261 catttcagcg aaccaccaac cggagacacc ctcgcccgca tccgtgagga catcggagac
    40321 tgcacgcgct gccggctcca ccagggacga aacaagatcg tattcggctc agggaatgag
    40381 caggccaagc tggtgttcgt tggcgagggt cccggggcgg acgaggacgc ccagggcctg
    40441 ccgttcgtcg gccgcgccgg ccagttgctg acgcaaatga tcgaaggaac agcgcgcaag
    40501 gaaggcatcc cacttgttcg caaggacgtg tacatctgca acgtggttaa atgccgccca
    40561 cccgagaatc gaacgcccct gcccgacgag atggagacct gcgggcagtt cctgttccgc
    40621 cagctttcgg tcatccgccc caaagcgatc tgtgtgctcg gtgcgacggc cgcgaaggcg
    40681 ctgctagacc gcaaggaggg tgttacgagg atgcgcggtc agtggttccg ctggcacgat
    40741 atccctgtga tggtgacgta ccacccgtcg tatctgctcc ggccttacaa ccagaacgcc
    40801 aagcgcgaag cctgggaaga ccttaagaaa gtcctgcatt atgtctatga ctgagcggcg
    40861 ccgcgggttc ttcatcactt tcgagggcat agacggctgt ggaaagtcga cccagatgtg
    40921 cctgctcgcg gaaaggctgc ggagagaggg ccaccgggtg ttcgaaaccg tcgagcctgg
    40981 cggcacatcg attggcaggc aggtgcggcg tatcttactg gacgcgaaga accaggagct
    41041 ctgcccgacg gctgaattac tcctctactt cgcctgccgc gcccagaacg tcgaagaatg
    41101 gatcctcccg gcgctcgaac gcggcgaaat cgttgtgtcg gaccggttca ccgattccac
    41161 gatggcttac cagggcgttg gccggggact cggcgaagat gttgtgcgag accttcaccg
    41221 catcgcctgc cgcgatctca cgccggatct gacgctcgtc atcgacatcg acctcgaaac
    41281 gagccttgag cgtgcgcgca cgcgcaaccg ggaactagcg gacaccggcg ccgcgcctga
    41341 gacgcgcatg gacgaccagg cggtcgagtt ccaccgccgg gttcgcggag cctacgctgc
    41401 gatggttcaa gtggaaccgg agcggttcgt tgtgatcgac ggccgggcgg acgtcgagag
    41461 tgtagccagg gccgtctggg aggcggtggc gccccgcgtc ggaggtctgc atgtttgacg
    41521 acttccacgg caatgagcgc gtggctgccg cgctagaaaa gatggccgcg caggatcgga
    41581 tcccgcagac gctgctgatg gttgggccgc agggtatcgg caaggcgacc ctggcgcgcc
    41641 gctttgccgc ccgcctgctc ggcggagcgg acaaaatcga gcgcgacgac ttgagcttgc
    41701 cgcacaacat cgaactccgc ggcgaacgcg agaagtggcc gtcggaaaaa cgggccgaag
    41761 agcccctcct gctggccagc cagccggatt tcgtgacgtt tcctccggac ggtccgctgc
    41821 gccagatttc gatccagcag atgcgcctgc tcaaggaacg tgctcagttt ctgcctctcc
    41881 atggccggtg gagaatattc ctcatcgatc agatcgaccg cgcgaacgaa caggccgcga
    41941 actcgctact caaaaccctg gaggaaccgc cggaccacct catcctgttc ctgactgcgg
    42001 aaaacgctta cgatctcctg cccaccatca ggtcgcgcgc agtaccgctg tatctggccc
    42061 ggctgagcga ttccgaaatt cgtaggttcg tggccggacg cggcgtgagt gacgtcgagc
    42121 gccgcgtgtc gttggctggc ggtagtcccg gccttgctct ctccatcgat ctggaggaat
    42181 acgacgcccg ccgtgaggcg atggtcgcgc ttcttgaagt ggccagcgga gtgtccccgt
    42241 tcagcgcatg gcttacatac tccgacaaga tcagcatgtc gcggcaggag aaactcgagg
    42301 actacctgaa agttctctac ggtctgctgg cagacctgct ggcggtgcaa cagtcggggg
    42361 ggggcattcg caatgtcgat ctccgcgcac gtctcgaagg actggcctca cgggtcaact
    42421 tcgactggat tcgcgccgcg accggcaagg tggacgaact ggtccgcctt ctgcgccgca
    42481 acatccagaa atccattgcc ctcgacgccc tcatccttga tctccggccg gttgtctgac
    42541 gcccccggac cgcccctgca aacgcttgct cattcagggg gtatgtgtta tcaataggga
    42601 aacgtaggcg ctttgatact tccgactacc gtctcgccgc atagttctgc gagcgcaacc
    42661 ggtgtcaaaa cgcatttata cataaggagt tatgccgtgg aaaccgccaa ccggagactg
    42721 atccgcccca gcctgaacaa cgccaaggag caggcggcct cccgccgaaa ccagacccag
    42781 aagaagacga tcccgcccga ccagacccac gcggaaagct tctattacct gaagcagatg
    42841 cagtccaaga ccccaatggt tgtggtgctc aaagacggcg aaacggtcca cggcatcatc
    42901 gagtggtacg accgctgctg cctcaaggta aaccgtgagg acggcccgaa cctgttgatc
    42961 tacaaaccca gcatcaagta catgtacaag gcgaccgagg cctgataggc tgcgctctca
    43021 gttcttcgcg cgtctccgca actggagccg gtattctcgt cgctggcccg cctcttcgta
    43081 acggcgtttt tcatcgcgcg tttccgggat gactggtgga atctccactg gttcaccttt
    43141 tccacccaga gcgacgaacg tgaggtacgc cgaggaggtg tgcttccgct cgccggtcgt
    43201 catgtcctct accatgacgg ttaccccaac ttccatcgag gtacgaaaca cacggttcac
    43261 cgaagagcgc agaacgacaa gctgcccgac gtgaaccgga tgaaggaagt tcatgtgatc
    43321 gatcgaggct gtcactaccg ggcgccgggc atgccgcatc gccgccagtc cgccggccag
    43381 gtccacgagg tgcatcaccc tcccgccgag catggtcccg agagcgttcg tgtcgttcgg
    43441 cagagcgaac tcggagtatt cggagataga ttctctgaca ggccgtgctt gcatcgccgg
    43501 tatcttatca tggggatcgg aaacctatgc ccgaagaccg catcgagatt ctgaaacgga
    43561 tggtcgccgc cagcccgaaa gacagcttcg cccgctacgg cctcgccatg gagtacgtca
    43621 aagcaggcga cttcgaggag gctgtccgcg aattccggga gcttgtcgtc ttcgatcccg
    43681 gctactgcta cgcatggttc cactgcggcc aggcactgga aaagctgggc cgtgctaacg
    43741 aagcccggga gatctaccgg caggggatcg aggccgcgga gcgcaagggc gacacccacg
    43801 cccgcagcga actccagggc gcactggaca tcctggggtg acaccgtcca gtcttcggcg
    43861 tctctcagaa gggtacgtcg tcgtcggtgg cgctgtagcc ggaagctggt tcctcttgtt
    43921 gtgccggggc aggttgcgag cgggcggaac tcctcggcat cgagaccacc tgagagtgtt
    43981 cgtcggcggc gggtgatgct tcgccgcgtc ccccaagcag gatcacgtct tcggctacga
    44041 cctcggtcgt atagaccttc cggccgtcct tgtcctcgta gctccgtgtc tggagcctcc
    44101 cttccacgta gatctgcttg cccttggtca ggtagttggc gaggttctcc tgccgccaga
    44161 cgacgacgtt gtgccagtca gtctcttcct tccactcccc ggtctgctga tccttccacc
    44221 ggcggctggt ggcaatggag aacgtggtgc gggcggcgcc gctgggtgtg aaccgggtct
    44281 cggcgtcttt gccgaggttg ccgacgagaa ttaccttgtt gacgcttcgg gatgccatac
    44341 ttccttgcac tgtagcataa gcgacctttg cggactacgc ggaccatgcg ccagcgcaca
    44401 atcgtcgccc gtggaacccg ccgaagagtt ttacaccggg tcgcggttga actttgtcta
    44461 ctttggtgca taatacggtt taggcgggta gggtcgatgc tgggtaggca ccacccgccc
    44521 gctgaaccgg acccgctggc cggcgaggag tgacagaaga acgaagagcg gcggtacagt
    44581 cccgatgaaa ccgtcccgaa tgagattcgt ttccgcgctt ttcctgggaa tcacgctggt
    44641 gactctctgt gtgcgtccgt cctccgttcg cggacaagtg ccgatcacta cgtccggacc
    44701 gtacggcgtg gtacgcctct ttggcagcga cgctgccgtt ctggaggtcg aggaaccacg
    44761 acgggatctt ccctgtactc tgacacccgg caaggcccta ctcgggtttg atcttcgcat
    44821 gcacgcagga tacgaagtgg cggttccgct gcgcgaacta tccggcgccg agaatatcct
    44881 caccatcgta ttccgggtca ccccagccgg gcgggacgag gatcctgtct atttctctca
    44941 acgggtccga gttcctccga tcgaggaaga tgccaagggc gacgcctacc ttcagggcgc
    45001 gttcgacctg ggtgaaggga agtatcacgt cgcctggctg atgcgggacc gcgccgagcg
    45061 ggtctgctcc tcatactggg acattgatgc cagtctcgcc tcgaaggaca aggaaatcgg
    45121 actcattctc cctccaggca ctgttcaggc gagcgagttt gagcccttca aggacgagcc
    45181 gccggtcgag cggacacaag gcgaacctcc actgaccgtc aaagtgctgg tcaactttgc
    45241 gccgcagaat atgcactccg ccgtcctgca gccgatcgat accagcgctc tggtctcgat
    45301 cctgcgcagc atcgttcgcg agccgcgaat cgcgaagttc tcgctcgtag ccttcaacct
    45361 tcaggaacag cgcatcgtct accggcagga agatgccgat cgtatcgact tccccgcgct
    45421 cggcgacgcg gtcgagtcgc tgaacctcgg caccgtcgat ctgcagcgtc tcagccacaa
    45481 ggatggcgag acccaattcc tcaccgacct tattcgcagg gagacggggg gtgatcgtcc
    45541 tccggacgct ctgatcttcg ccggtccgaa agctatgctc gacacgaaag tcgccgacga
    45601 ctccctggcc gagctcgggc cgctcgatta cccggtcttc tacatgaact acaacctgaa
    45661 cccgcagttc aacccatgga gagacgctat cggccacgcg gtaaaattct ttaaggggac
    45721 cgaattcacg atcagccgtc ctcgcgacct ttggtatgcc gtaaccgaca tggtgtcccg
    45781 catcgtcaag tcgaagagcg agcgacggac caccgcggca tcggcgaggt agactatgcg
    45841 gaccgtaggt atcttaccga ttgccgttca atcccttctg ctcttcgctg cgacgctgag
    45901 cgctcagcaa cgagtacatc ccgacaatct ggcgccattc gttcccactc cggaaccggt
    45961 tgtggagaag atgctggaag cggctgaact gaagtccgac gagacgctct acgatctcgg
    46021 ctgtggcgac gggcgcattt tgttcgtcgc agcccagaag ttcatggcga aggcggttgg
    46081 cgtcgagctc tccgcccgtc tggtcaagtc gaccaccgaa aaggcagaga agctgggatt
    46141 ggccgaccgc gtgtcggtga tcgagggcaa tcttctcgac gtggatataa gcccggcgga
    46201 cgtggtctcg atttacctgc tccggctgtc caatgaacgc ctcaagccga acctgaaaaa
    46261 gcaactgaag ccgggcacgc gcgtggtttc ccacgactac gaaatcatgg gctggaaacc
    46321 ttaccgcacc gagaaagtgt cggtccacca gcggacgcac acgatctacg tctacaagat
    46381 gcccccggta gagcagtagg gccgcgctgt ccgatccgcg ctacaatcgt caagccccac
    46441 accatgacga gcgctttcga tgtcgctggc gtcggtctga atgcgaccga cacgctgctg
    46501 atcgttcccc gtttccctcc ctacgcaggc aagattcttt tcgaagagga gattctcagc
    46561 ccaggcgggc aggtggccag cgccatggtg gcgtgcgccc gtctcggtct ccgcgtgaag
    46621 tacatcggaa cggtgggcga cgacgaacgc gggcgcatcc agatggcaag tctgaaggca
    46681 tcgggcatcg atatcgagga cgtgaaggtc cggccaggct gccccaacca gtcggcctac
    46741 attctggtcg accgttcgac cggagagcgg acggtgctct ggcggcgccc cgactgtctg
    46801 cgccttgatc cctcggagat cctccccgaa cagatcctcg cttgccgcat gttgcatatc
    46861 gacggccacg acactcccgc cgtcgaaaaa gcagccagga tcgcccactc cgcgtccatc
    46921 cccgtaaccg tggatgtcga cacactgtac cacggctttg acagggtgct gccactggtc
    46981 gactatctga ttgccagctc cgggttcccg gtccagtgga ctggcgagga cgatccgttc
    47041 aaggccctgg aagtgctcca gaacgagtac gggatgcgcg tcgccgcgat gacgctcgga
    47101 gcccacgggg cgctggcccg tatggaacgg cgattcgtct actcgccggc gtacgtggtc
    47161 aactgcatcg acacgacggg cgccggagac gtgtttcacg gggctttttg ttatgctgtc
    47221 ttgaagagca tgcctatcga ggaggcgcta gacttctcca atgccctggc agctctgaac
    47281 tgcacggctc tgggtgcgcg agggggcatc cgcggtgtgg aagaagcacg agccctgatg
    47341 ctccgcgcgg agcgccggtt ccatcccgac ttcgcctcgc acacgggacg atagcgcatg
    47401 atcccactcc gagactcgca gcgcagcaat tcgacgccgg tgatcacggt cgggttgatc
    47461 gtggtaaacg tccttgcttt tctgtaccag acgtcgctcg acaagttctc gctgaaccac
    47521 gtgatcatgg agtacggtgt cgttccgagc cggctccagc tcacctcgct tgtcacctcg
    47581 atgtttctcc acggcggctg gatgcacctg atcgggaaca tgtggtttct gtggatttac
    47641 ggcgacaacg tggaggacat ccttgggcac gcgaagtacc tgattttcta cctggcctgc
    47701 ggcgtggctg ccgccctggc gcacgttgcg ctgaacgggg actcgcgggt gcccaccatc
    47761 ggcgccagcg gggctatcgc cggcgtaatg ggcgcctaca tggtgaagtt cccccgagcc
    47821 cgcatcatca ctcttatctt tctgttcgta tttgtgacga cgatcgagat gcccgcgtac
    47881 gcgatcttgc tttactggtt cgtcatccag atcttcagcg gcgtaggttc cataggctac
    47941 tcgcagattt cgcagggcgg tgtggcgtgg ttcgctcacg tcggcggttt tgtaaccggc
    48001 atcgtcctgg ttatgctgat gcgaacgcgg gaaccgtatc gcctgcatcg ggaactccac
    48061 tggtaatgga cactccggct cacccaatcg acattctcgc gatcgctgcc caccctgacg
    48121 atgtagaaca gacctgcggc ggcactctga ttcggatggc cgaagccggc taccggaccg
    48181 gtgtgctcga tcttaccgcc ggcgatatgg gtacccgcgg gacgccggag ctccgcatac
    48241 aggagtccgg ggaggcggcg aagcatatgc tgctctcctg gcgcggcaat gtacggatgc
    48301 ccgatgcgcg cctcgaaaat acgctggccg cccgcatgac gctggcagtc aagatccgag
    48361 aactgcggcc ccgcgccgtg atcctgccgt actggcaggg aaggcacccg gatcactatc
    48421 gggcgagcga gatcggctat gaggcctgtt tcctggcagg cttgcggaag attgacgaat
    48481 actcggagcc gcaccgcccg cacaagatcc tctacgcatc gatgtacgcc aacgtcacac
    48541 cgtcattcgt tgtggacatc agcgcccaat tcgaacggcg catgtccgca cttctcagct
    48601 accgttcgca gtacggagag atctcggatg gggccgctct gttcccgaac gaatcggaga
    48661 tacgcgagcg acatgcggcg atcgcccgct tctatggcaa tctgatcggc gttcgctacg
    48721 gtgagccgtt cgtggtgaag gagacgatgc gggtggatga cgtcgtcgcc cttggcccgc
    48781 ggagtttgtg aaacgctgcg gtcgctaatc cttatccaac ggggttacgg ggtcgatgaa
    48841 cggccagcac agtgtgccca acagatagac gccggccatg atgtacaggt tcacgttcca
    48901 gtttccgtcg gtccagcgca gcacgtagcc cgtcacggtc ggcgcggcga acccggccag
    48961 gttccccatc atgttcatcg agccggagag cgtgcccgcg tacttgcctc ctacgtccat
    49021 gcacgcgccc caggcgccgg gcatcaccag gtcattggcg aagctcgcca tgcccatcgc
    49081 gaccatcgcc aagagcggat ccttcaggtt gatcgacagg atgatcaata atccagcgcc
    49141 ggcgaagccg atgcacgcga gcagccggcg ggctgtgcgt gtgctgccga gccgccgcgc
    49201 cacccccgcc gagaccagcc cgcagaacag cgagccgaat ccaccgaaca gcagcggaaa
    49261 gatcgcgagg cgcgacgcct ggcgcgggtc gagaccccga gattcctgca agtacgtcgg
    49321 cagccacgtg atgtagaagt accacggata cgacaggagg aagtattgcg cccagagcag
    49381 ccagaccgtt cgagaggaaa ccagcttctt ccagggcacg tctccgtggc cggaagccat
    49441 cttctcggag ccggcaagca aagccagttc cgccccgttg acgctcttgt ggtctcgtgg
    49501 gtcgtctttg aaccagcgta aaaagaacgc gcaccacagc aggccgagga gaccgaacag
    49561 cccgaacgcc tgccgccagc tcatgtagtc aaataccagg attaccagtg gcggagtgaa
    49621 tgcgccgccc catcgggcaa acgtccacat gatgccctgg gcccgcaccc gttcgtgctg
    49681 gggcagccag gttgtgaacg ccttggtcag gttggggaag cagcccgcct cgccggcgcc
    49741 gaacaggaaa cgcacgcccc agagagaggc catgttccag acccaaccgg tcgccgcagt
    49801 gaaaaacgac caccacaata cgatccgaac cagcacccgc ttgggtcccc aggcgtctcc
    49861 catccatccg ccggggatct cgaacagcgc gtaggcgagg gcgaacgccg agaagacgta
    49921 tcccatctgg actttgctga gtccgagatc gcgcatcatg ttcggcgccg ccatcgaaat
    49981 gcagacgcga tcgatgtaag cgaggatcga gagggtaatc gcgaacacga tcacccagta
    50041 gcgcgcgcgg gtggctttct gagcgagttg actcgctgac acgatgcggc ccctcctggc
    50101 gagaaacgga tatcacacca tgcggcggct tgcggatgca agcgagactg ctactcgcgg
    50161 gctccgagtt ccttccccag ccatgtgagg tagcccggcg agccctccac gatcgcgacg
    50221 gccgccacct ccggaacttc gtacgagtgg agtttttcga tctcggcacg gagcgcgtca
    50281 aacagcccgc gcctggactt gatgatcagt agcgactccg gtgcgtcttc gatttcgtct
    50341 ttccaccggt aaatgcttcg ggcgccgggg acgacgttca cgcatgccgc caatcgcttc
    50401 tcgaccagtt gccggccgat tcgcgcagct tcttcggctg tgctacaggt gctcaatacc
    50461 acgattttgt cggtcatcga gacctcttta tttctcgaaa tgctggaaaa tccggcaaaa
    50521 gaggattata aacaagagtg agggtgaacg acttgccaaa ttcccgtgtg aggaagattg
    50581 ttcgtgccgc gtggcctctg gtcgccctgc tgggagtcgg agtctatgtg tacgcaacgc
    50641 ttcgaagtcc gcaggggctc tccgcggtgc tggaaaaaca gcggcaaatt cgcgaccttc
    50701 agcagcagaa cgcgaacctc gatcgcgaca tccagcagaa acgggaacgc atccgcaagc
    50761 tgaaggaaga ccggaacgag caggaaatgg tgatccgcga gcgctatcat ttgctcaaag
    50821 agggtgagac cagcttcatc cttcaagatc agaaaaagta atccctcaca agtctcccgg
    50881 ccttcaatcc ggcccctttt tcgatatact gtggccggtc tgccccgagg gctgtccagg
    50941 agaatacgtc catgcgatct accaaactca gccgccgcga tttcgccgga attgcgctct
    51001 gcggaacggt ggcggcgagc accgctgcct ccgcctcgaa gctcgagcca aatccgcccg
    51061 gcgtcaagat ctccatccag tccggcagcg acccgacaga tgacgacttg aacttcgtcc
    51121 ggcagctcgg aatcggttac atcaatctcg ggacgcacgg cgagcaggca acggccgaga
    51181 acttccttcg gctgcgcaat cgtgcggaat cgttcggcct gaaggtctgg aacatcggca
    51241 acaccgacgt ccataacatg gaggaggtca ccctcaatct tccgggacgc gaccggaaga
    51301 tcgagcagta caagaactac ctccgtaacc tggccaaggc gggaattctt tacaccacat
    51361 acgcccacat gggcaacggg atctggagca ccgcgccgga aaagatccgc ggcggcgcca
    51421 ctgctcgcgc cttcgacctc gcgaaggccg agcagggtgt atgggcaggg aagattttca
    51481 agggaccgct gacgcacggc cgcaagtaca cgaaagaaga gatctgggac aactacacct
    51541 acttcatcaa gcaggtcaca cccctcgccg aggaactggg catccgtatc ggaatccatc
    51601 cggacgaccc gccggtgccc gaactggggg gcgtgccccg ctgcatcttc agcagcttcg
    51661 acggctacaa gcgcgccctc gaaatcgcca acagccccaa cgtgggcatc tgcctctgtg
    51721 tcggctgctg gctcgaaggc ggaaagctga tggggaaaga tccggtagaa acgatccgct
    51781 acttcggcag gcagaaaaag ctcttcaagg tgcacttccg caacgtgact gcgccgcttc
    51841 cgcacttcgt tgagaccttc atcgatgaag gctacttcga catgtacaaa gtcatgaacg
    51901 cccttgtgga ggtgagattc gacggcaacg tgatccccga ccacattccg gcgatggtcg
    51961 gcggccgcca cgcagccacc gcgcacagca tcgcctatat gaagtcgatg ctggaacgcg
    52021 ccactacgga agcctgcggg cgggcatgac atctgctggg cggatcgcga tacaggcgtt
    52081 cggactttat ctgaccgtct gcctgcttgc ctccggagcc gagttggctg ccgatcaccc
    52141 tcgcctcgtg ttccagccgg gggatcgccg tgggcatggg cgcacgttcg aggaaatccg
    52201 gaagttgcac cgggacgacg gcacgtttcg ctccatcatc gagaaggccc tggcgctgga
    52261 tctcgagaaa cagcacccgg cgatgccggc ggcctcctgg atcgtctccg gtgaggaccg
    52321 ctacgccgag gccgcgatcg aaaggatgct gtccgcgccg atcagcagga gcggcgaagg
    52381 ttcttattcc aacatctggt cgcacgcgct tgcgtacgac tggctctatc atcacccgcg
    52441 ttttacaccc gagaagaaag agcgtgccgc gacgaagatc gttgagcggc tggcgacaga
    52501 actcgcggga cttgacgata gcggaatggc cctctggcac ggccgcaacc aggcggccaa
    52561 cggagctatg gtagctgcgc tggccgtagg cgatctgccc ggccagcggg agaacctcac
    52621 gcgggccacg gcccactata tcgaatctct tcgcgcgctt cggttctccg agggttggcc
    52681 ggagggggcc agctactgga tctacaaccg cgcgggcccg tacgccctcg ccgcggactg
    52741 tgtgatgacg gcgctcggtg tcgactccat cggaggcgtc cccatccgcg aggtgatgcg
    52801 caagatcggt ctctggtcgc tctatcagtt tgctccgaac ggagtgtttg agccgtacgg
    52861 cgactctggc ggctctctcc aacttggcga gacgggctgg tgggaactca ccaccgacta
    52921 tttcgcgcgg ctctcgaagg atccctccct catggccggc gccgactact taaggaaccg
    52981 gtccccaaac ccgtacggcc gcaggccgta caactggtgg gccgtgctga gctacgatcc
    53041 cacggcgcgc ccgcgccgcg gctacgaccc ggcgaagcca gagatctgga tgcgggcgcg
    53101 tttgccacaa tcgatgctct tcggacgcga ttcgattggc gtcgccttct ttcgcggaga
    53161 gtggggtgat cccgacgagc tctacgccac attcaaggca ggcgatatgc tggcccacca
    53221 tgaccactac gatgtgggcc acttcggcat tcagttcgga ggactgctgg cgccgcagac
    53281 cggaatctac gggtcgggta cggatggata cgacggcgcc taccgtttgg gttacggtgt
    53341 ccagtcggtt tcggtaaaca gtctgctgat tctggcgccg ggtgagattt ccgccgactt
    53401 gcgctcccgg aagaacccgg cctggacctc gctctccgga gggcagcgag tcatctcgcc
    53461 gaccggcttc tcgtgcgtca gcctggacga ctttcgccac aagcttcaaa cgggccgtca
    53521 tctcgaacgc ggcggcatta ctgtgttcga aagcatcgct ggcgaattgg actacattgc
    53581 ggctgacatc acggcgtcat acaactccac aaaatgggcg gagcccggta ccgagccaaa
    53641 ggtctcgctg gtcacgcggc agtttctgta cctccgccgc gagcgagcct tcgtggtcta
    53701 cgatcgcgtg gagaccacga atgcgcgttt cctgccgaag tttctgctgc acagcctgtc
    53761 gaagccggtg acgcaaagcg agaagttgct tgccggcagc ggccctgaag acggtattct
    53821 cgagacaacc gaccgaaccc tgcgcaccgc cgatcagcga ggagaactca cgcaactggt
    53881 attgttgcca cgcgacgcac gggcgctgaa gatcggtggt ccaaactact acggctatgt
    53941 cgagagcgat ggcgaccagg cggacggttt cgacggagtg aatctaagcg ctggagatcc
    54001 gcgtataccg cgaaagaccg ctcagcttgg attgtggagg acggaagtgg agccggtcct
    54061 gtccggcacg agcactcgct tcctcaacgt cttgcttccg cggcttgtgg cccgacgcgg
    54121 cagcctgccg gtggtggatc ttgcgggatc cggcgaaggg gccgatgcgg ttcgggtcgg
    54181 caacaccgtc attgtgtttg ctcgacggcc aggagggctc gatgcggtcg aactggacct
    54241 gagccagccg gcagaggtca ttcttgtcga tgccgtgccg ggcgtgcgtt accgggccgg
    54301 cggccgaacc gcgaccgcta gtggagaagg cgtgctgcgc gttccgggac tggctagggg
    54361 gaggcaaacg gtggcacgaa ccggacgcta gaatgcggtt gacagcgccg tgcggatggt
    54421 gccagtctgg gacagcatcg gaaggaggca gcgatggcgc tggcgattgc ggttcgccaa
    54481 gttggaagcg tggctgtgat ggatgtgagt ggaaggctgg tgttggggga agagtgcagc
    54541 attgtccggg agaagatccg tgaactcctg gcgtcaggcc agaagaacat cctgctgaat
    54601 ctctccgagg tgacttacgc ggacagcgca gggctcggcg aactggtcgc cagccacgtg
    54661 acggtcgcga aggccggcgg caggctaaag attctcaact cccagaagaa gctcgacggg
    54721 cttctggaaa tcacgcggct acacacgatt ttcgaggttt ttgcggacca ggacctcgcg
    54781 gttctcagtt tctgagtccc tatccgcggg cttacttctc gagccgcaac cttccaaagg
    54841 cctcgggcac gtggtagttc atttggccgg tcggctgcca agcgacgtga acccgtttcg
    54901 gcggcggacc ctgaatgcga tacaggttgg ctcgcatctc gaggcccggc gccggcttcc
    54961 gggagtcaat cgaagccatc gggatctgca tctcgccgta ccagattttc ttcttttcgt
    55021 cgatccgcgc cttcaccttg aatccggaat tccagagcca gccgccttcc ggcttcgggt
    55081 tgtcgcggtc gatgtccaga tccacccact cgccttgcgg cgagacctgg aactccttgt
    55141 actggcggat gtgtttgaag tccgtcccga taaacgcctc cgcgacatcc caatcccaga
    55201 gcttgttggt ctcggtcgtc gtcgacgggt tgggcttcag gaatagcttg tcgtaaggac
    55261 agatgtacag aaagtagaga ttcttgtcgg tccaccgcga acgaatctcg gtgcgatgcc
    55321 cgggcgatgg ctccttcgtc ggcccgttct cggcaaacac cccggctacg ttcttccatt
    55381 gcgccgcgtt cggatcagcg gtcagggcga agtccttgct cgaatagtag ctcttgatga
    55441 cgccgggacc ctccgccaga gccacgccgg cgacaaacag gaagagaagg aatgttagct
    55501 tcatttcctc tccaatataa gctttaatag cggtatgcga aacctggtgc tcaccctgtt
    55561 tttactggcc gccctttgtc cggcacagtc gttcgagccg gtcaccacgc ttccggactg
    55621 ggtctccgtc gaagccaatg tgccgtacga ccagtacaag gagacggttc ttgacatcat
    55681 gcagccgaag gccgcagcga caggcaagcg gcccggcatg ctggtgatcc atggcggtgg
    55741 ctgggtcgga ggaacaaagg aacaagtcgt agagagcttt tgcttgccat acgtcgagaa
    55801 aggctttgtc gtggcgaatg ttgagtaccg gctggccaaa gtcgctccag cgcccgccgc
    55861 cgtcatggac gtgctcaagg ccgccgactg gttccgccgc aacgcgggcc gctacaacgt
    55921 ggacgggaaa cgaatcgcgg tgatcggcaa ttcggcggga ggtcacctcg ccctcatggt
    55981 ggggatggcg tcgaagtcgg cgaaactcgg gccgaccggc aaagtcgccg ctgtcatcaa
    56041 tatttacggg atttccgacg tagccgacca gttgggcggt ccgaatacgc gcgattatgc
    56101 ggtcacctgg gttccggacg aacccggacg gctggaactg gcccgacgcg tttcgccgat
    56161 gacgtacgtt cgaagagatc tgccgccgat tctcactgtt cacggcgacg ctgatgagac
    56221 ggtaccgtat gagcacggca caaacttgac caaggcgctg cgccaggccg gcgccaccgc
    56281 cgagatgatc tccgtgcccc agggcaaaca cggattttca aaggagcagt gggccggcgt
    56341 tctaccgcag atcttcaaat tcctggaacg ccaccgcatc ttgcggtaag acccgtgtgc
    56401 ccgcaccgca ggtggtcagt cttttgtgac cgcttcctgc cagcggctga tcgcggtggc
    56461 gagctcgtcc acgagttccg gatgcttgcg ccggaggttg cgcgtctcgc cagggtcttc
    56521 ctcgagatcc gaaaggaaca tcgcgtcgtc gcccctgagc gggtttcggc cttccggcgt
    56581 cccgtcgtag actgccccgt tgacgaccag tttccagcgc cctcgccgga ccgcctgttg
    56641 tttgtcggaa tcccagaaga cgtcaggatg ctgtgacttc gcccctgccg tcatcagcgg
    56701 caaggcgtcc cggccgtcga tggtccggcc cgccggcaac ggcacaccgg cggcggtaca
    56761 gatcgtcggc aggagatcgg ccgtcattgc cgtctcccgg agcacttgtc ccggctggat
    56821 cacgcccggc caatgaaaga ccgctggcac gtgcattccg ccgtcgaaaa gactaaattt
    56881 gaaaccccgg tagggagaat tcctgcctgc tgttgccgcc tgctggctca agcccgcacg
    56941 cgcctccgtc gtcgcgccgt tgtcggcgct gaagatcacg agggtattgt cgcggattcc
    57001 tcccctgtcc agcgtccgca agacttcccc gacgccgtcg tcggccgccg caagcatcgc
    57061 tgcgtacatc cgccgctccg gttcgagctc cggaaagcgc tcgacatact tcctcggtgc
    57121 atgcatcgga tagtggaccg cgttgtaggc gagatagagg aagaacggct gggagtggtt
    57181 tgcctgaatg aaaccgaccg cttcttgcgt cagccgctcg gtcaggtact ggccgtcctc
    57241 gaagatctcg gttcggtttc tccacagatc gtggtagttc actacccgtg gctcgcccca
    57301 gtagtaccgg tgcgagtaga aatcaacgca gccggagtga aagccgtaga agtagtcaaa
    57361 gccccggctg tttggcgccg tttccggtgt ggaaccaagg tgccacttcc caactaggcc
    57421 cgtcgtgtag ccggcgggct tgagcagcgc cgggatcgtc tgttcccgca ccggaaggct
    57481 catgccgttc cgcggcatgc cgcaccgcag cgggtaccgg cccgtcatca acgtcgcgcg
    57541 ggcgggcgcg cacatcggag cgttcgaata ccagttcgcg aaacgcgcgc cgccggcggc
    57601 cagcgaatcg atgttcggag tcttgacatc ggttgccccc tggcacccga ggtcatggct
    57661 gcccatgtcg tcaagcagga tcacgatgac gttcggcttg cgctcccgcc ctgacgaagc
    57721 ggccaccccc gctcccgccg ccaaaaattc tcgtcgattc agcatctcca cctcaccgcc
    57781 gctgcaattt caggactacg gacgcgattt ccatcgtcgt ctcttcgtcg cccatcagta
    57841 cccgatgtgg cagccagaac gcgtcatgga tgcaggcctc cgacaccggg cacggctcga
    57901 cgcggcatgc gcccttctga tagagcggat tctgatacaa aacgtgcgga tagaacggcg
    57961 tgcagggaat ctcccgctcc gtgagggcgg catggaactc gtctcgcgtc attccgaact
    58021 ttgctgcgtc gatgcgcccc agcagcaggt agtatgagtt cacgttgacc tgggacggga
    58081 ccgcctgcca agtgaggccg tcgacgtccg aggtggcggc gcgcaaccgg caggcattct
    58141 ccatacgcag gcggatctgt tcaggcagcc gttccaactg cgcgatgagc accgcggcct
    58201 ggagcgctgt aatccggtag ttcgtcccga gcgtgaaatg gtggaaccag ccctcgccct
    58261 cgcggcgacc ctgattggca atggcacgcg cccgcgccgc gatgccgtcg tcattcgcgg
    58321 ttagaatgcc gccctcgccg gcggtcatta ccttcgagtt ctggaagctg aacgtgcccg
    58381 ccacgcccag actgccggcg cgcctgccag tccactccga accgtgggcg tgggctgcat
    58441 cttcgaacag cattaggcca cgatcggcgg caagcttcat caggcggtcc atgtcggcca
    58501 gcggcccacc aaaatggaca ggcacaatcg ccttcgtacg cggaccgatc gccgctgccg
    58561 cgcagtccgg gtccatgttg aagctgtacg gctcgatatc gacgaacacg ggcgtcgcgc
    58621 ccacgcgcga taccgccgtc gccgtcgaaa tgaacgagat cgcggggacg atcacctcgt
    58681 cgcccggccc gattccggcc gcctccagcg ccatctcgat cgccaccgtc ccgttggcgc
    58741 aactcacgcc gtgacggcaa tgttggtagg cggcgaaatc gcgctcgaag cgctcgacca
    58801 tctcatgaaa gccaccccac tggaggctgt cgatgacctc cttgagcaac tccatctcac
    58861 ggtccgtggc gaggggccag cgcggaatgt tcataaccgt atcagcatac cggggtcaga
    58921 gcccctgaag aaagtagttg aactccttct cgcggccgat cgtcgttgtg tcgccgtggc
    58981 cgggtaacac cagcgtctcc tcgggcagag tgagcagctt gtcccggatc gaaacgagga
    59041 tcttacgaaa atcgccgcca ggtaggtcgg tgcgcccgat gctgtcccgg aacagtgtgt
    59101 cccccgcaat cactttgttt tcggacggga gccacaggca gatgctgccg ggcgtgtggc
    59161 cgggcgtgtg cagaaccgag aaatcggagc ttccaagccg tacagtgtcg ccgtcccgcg
    59221 tctttccgtc gatttccacg cgccggggcg tggccatacc gagccacgat gcctgcatat
    59281 cgagttggtc gtaaacgcct tcgtcgtcct cgttcatgag tacggaggcg ccggtaacgg
    59341 ccttgagctt cgctgcgccg ccgacgtggt cgatatgtgc gtgtgtgatg acgatcagag
    59401 tggcgcgaag cctgtgccgt tcaagaatcc ccaggatctc ctcgacgttg tcgccggggt
    59461 cgatgacaat cgcttcacgc gtctgttcgt cgccgaaaac cgaacagttg cactgaagca
    59521 tcccgacggg cagaacctcg tgaatcataa acgtgattgt agagcaccga gccggttgga
    59581 gactccggcg cgtcatgctg ctagactggc tcgaatgggc gccatgaggg gcgtggctga
    59641 agcgctctcg gccttgcgta ccatgctgga ggccgagacc ccggttggag actccgcgag
    59701 gtctttcctc gatcttacgg tccggctaag ggactcggtg cagccggtgc tgggcggcac
    59761 ttgcacaacg gcgtgcgacc ggctggccga acgtgcgcgc gtgctcgaga gaaccggcga
    59821 tcttgaagcc cacctgctcg ccggcgttca gctcgaatcg acactcgcct gcatcggcga
    59881 ggcaatcgcg gacaacacgg aagcaaaagc gcagcacgtc cagcaagctc tggcgcggct
    59941 cctgcaacgc gcctgtgtcc tcgccgccgg cacttcaagt ccggattgga attcctttcc
    60001 ggatctgata tcgacgctgg cggttgttcg ccgcagcgcc ggggagctcg ccggactggt
    60061 gagcacttgc ggaggcgact tagctcggaa tgccgcggaa ctgaatcaac ttttggagtc
    60121 ttccgaagct cggttgctcg aagacccgtg cgcaatggtg gcgtcactca cgagcgaact
    60181 gaggtggcat cttggactcg ctgtccggcg ggactggtgt tttctccacg aggacatgga
    60241 cgattcttcg ctagcggcat acaccaacac gtggagggaa atgctgcttc cagccgatgc
    60301 gcagattact ccggagggga tcctcgacgc ggtctcgctg tcacaacagc agttccggat
    60361 ctgtgagata ccagacctcg atgattacct tgacgagccc gagcacctga tcggctacgg
    60421 tctggcgaac ctgatcccgc ctgcgccgta cggctgggag ttctcgtccg ggaccgcggt
    60481 gcaggccttc ttgcgaggcc agctcgggca atggtacatg cggccgtttc gcgacggtgt
    60541 ctccccagtc gagatgatct ccactgtgct gaagttcggc aggccccttt attacgagcg
    60601 aatccttctg cactccgtcg cacagcacag gctcgttcaa catgcggtct ggccggatgg
    60661 cggcggtgtg agccagttgg agaccctcgc gcacttggaa gatttcttca atgtgatgct
    60721 ggacggctat ctgctccgta agatctcgat gcccaacctt cgacgacccg gcgggtggat
    60781 cgagtaccta gaggatctga ttgctctcca tttcgcggtg acgccgcaaa aagtcgtcga
    60841 ggcccgtaag gactttctcg cgcgtcgcgg tttcgactcc ggcgcccagt tgctcgaatc
    60901 cggcggtcac ggcggggtct tcctgaatta gcccgtatgc tcgaatccgc cggcgtggcc
    60961 attctacact tgaggtatga gcgggatgcg gatggagggg tatcgtgcgt tacgcgaagg
    61021 ggcagcatgg ctcgatctca cggggcgcgg aaagattgcc gtgcgcggcg aagaccgagc
    61081 tcgcctgctc catgccctgt gtacgaatca catccaggaa ctcacgccgg gccggggatg
    61141 ctacgccttc tttctgaacg cacaaggccg catcctcggt gacgcgaacg tcttctgcct
    61201 gtccggccac ttcctgctcg acaccgaacc ggaactcgcc tcgaagcttt acgatcacat
    61261 cgacaggtac atcatcgctg acgacgtgag cctggaagac atgacgggcg gcctggcgac
    61321 gatcgggctg gaaggcccga aggcagcggt tgtcctttcc gccgccgggg cgacaatccc
    61381 cgagacaccg tacgcccacg cgccgtgggg agatcgcatg atcgccaggg cgaacacaac
    61441 cggctccggc ggatttctgc ttttcgtgcc cgccgccgag aaggacgcga ccattagggc
    61501 gctcgaggcc gccggcgctg tcgccgcggg ccctgacgac actcgcaccg tgagggtgga
    61561 gaacgccgtt ccgcgttacg gcgaagacat cacggaagcg agtcttcccc aggagacgcg
    61621 tcgcgacgat gcgatcaatt tcaacaaggg ctgctatctt gggcaggaga tcgtcgaacg
    61681 gattcggtca cggggacacg tgaatcgctc cgtggtccgc ctcgaaatcg aaggcgacca
    61741 ccccctcccg gcaggcgcgc gcatcctggc aggagggaat gagaccgggc agatcacgtc
    61801 cgcggagttt tcaccggctc tcggtaatac ggttgccttg gcgtacgtcc acgccgtgga
    61861 tgcccgtccg ggcacgcgtc tcacagtgga cggcgcggtt gctgtcgtca gcgtctgagg
    61921 tggtcgagca cttccccagg aatcgccaga tggccggggt caacgagtca tagccgggat
    61981 gccgttcgga atcaagccca actgctccag cttgctccaa accttgtccg aactctcctc
    62041 cggcgtttcg cgggaggaat ccacggtgac ctcggggttc agcggcgcct cgtagggatc
    62101 ggagactccg gtgaactgcg ctatctcgcc agctagggct ttcttgtaga gacccttcac
    62161 gtcccgctcc accagcacgt cgagcggaca atggacgtat acttcgacga aattggcagt
    62221 ctgcgcccgc acttcgtcgc ggatcgcccg atacggcgaa atggccgcgg cgattgcgat
    62281 gaccccgttg cgtgagagga gatcacaaac gaatccgatg cggcggatgt taatgtcgcg
    62341 atcctccttg ctgaagccga ggcccttgga aaggtgtgtc cgcacgacgt ccccatccag
    62401 tacctccacc ttcatacccg cttcgcgaaa tcgaccagcg aggatctcgg atatcgtact
    62461 cttaccggcg ccggagaggc cggtgaacca cagcgtaaat cctttatgca tttgtccctt
    62521 gcagaatatc attgaccagt acggccggtc cacgcaagac tgctcctcca ttcccgaaat
    62581 ggataccaca ctccttgtgc tcgtcctgtt cccaccacca gcgccccgcg cgctcgtcct
    62641 ctccgggcgc aaccggccgg gtacacggcg cacagccgat actggcataa ccttgcgcgt
    62701 agagcgggtg gaccggcacg tcgtgccgcc ggatatagtc ttccacgtcg ccgccggtcc
    62761 agtcggccag cgggttcagc ttcaaacgcc cgttctcttc ctcggccttc ggaaccgacg
    62821 cccgggtcac tgactgggac cgcctgagcc ctgttgccca agcccggaac ccggcaagtt
    62881 tccgctcgag cgggcgaacc tttcggattt cgcagcaaag ctggcgcagc gcaggctctt
    62941 ggcggaagag gttaacaccg taagagcgca ccatcgcctc gagttcggcc ggatccggag
    63001 aagccgtctc aacccgtacg ccgtacttct cgcggacggt atcgatcatt tggtgtgttt
    63061 cttccggcaa acggccggtg tccagggtaa ccacccgcac ctccgtggag attcgtgcag
    63121 ccatatccag aatcaccatc ccggtcttct ggaaactagt gcaaatggca aacgactcgc
    63181 cgtacgtccc gatcgcccag cgcaggagat ccggcgccgg cagacgctcg agctgagaga
    63241 tgtcagtcat cgaagaccct aatctctatc agattagtct attttgtgat gcaatgcaag
    63301 cttaccggag ctttgacctc ttgccgaagc ggcttgggga ctaccggacc ggcccgtcgg
    63361 agatgggatc gacgtcgaat tcgaacaact ggcccccggc ggtgaagcgt agtttgccgg
    63421 cgccaagcgg gcggcccagg gtggggaaaa agaccgcgcc gtcggtcgat tcgccgggct
    63481 tcagtcttgt ttcgacgaat gcgatggcgg aggcggctgc ggcggccttc aacttcgtgg
    63541 aggcgacttc agcaagggct gagcgtcggc gctgttcgta gccgttcgtg gaacggaacc
    63601 ggaccatacc gtatagcccc atctcgtagg tcgaggtgag cttgacaaca tcgttcctgc
    63661 cggctctatt cagaagttcg ccgacgacgg attccgccga ggaggcccgg actgcaaagc
    63721 cgtcgtcgcg ggtaaagacg aagtcttccg gacggaccat gcacgggatt gtcgcgccgt
    63781 tggacgcagc cacctgcagt atggagtact cacggacctg gatcggcaga tgggcaaaca
    63841 tgactgttag gccgttcttc gagagagttt ggtacttgag tccaccggac tcaaactcga
    63901 tcacctgcgc agccacgatg ccggccacag cgatcaggag gagtacggcg cgaaggagca
    63961 tacccactat ctatgatagc gacatctgaa aagaaaacgc tgccacgttt gccagcgcgc
    64021 aggccccggg gcggggaagg tgacgttccc cggcgtggat ggattcgcgt gcaatcttac
    64081 cgcttcattg tgatggctag gtggctgtga taccgtggag gatcagcgga aatccgtagt
    64141 cggaaggagg actcatggaa gccctcggga tgatcgaaac caagggactg gtgggcgcga
    64201 ttgaagcggc cgacgcgatg gtgaagaccg ccaacgtgat tctgaccggg aaagaatatg
    64261 tcggggcagg atacgtgacg gtgaccgtgc gcggcgacgt cggcgccgtg aaggcggcca
    64321 ccgacgcggg agcggccgcg gcccgccgcg tgggcgagct cgtctccgtc cacgtgatcc
    64381 cgaacccgca cgaggaagtc gaaaaggttc tcccaggggc ggaaaaaggc aaaaaatccc
    64441 acggctagcc gccgggctga accccaggct ttccgaagga gcgccggttt cctgccatgc
    64501 ctcacgaaca agaccccgcc gccatcgagg aagtccgcag caaggtcgaa aaagcctatg
    64561 ccgcgtggct gaagtatcgg aacttcacac aggaacagat tgacgtcatc gtagagcgcg
    64621 cggcggcagc gggccgcgct cacgcccgcc gcctcgccga gctggccgtc gaagaaaccg
    64681 gttacggcaa tgtcgacgac aagatcgcca agaacctact gtgcgcggag ttcctgccga
    64741 aggcgatgcg gaaagtgaag acggtgggcc tgctgcggga gatccctggg cagaaggtcg
    64801 tcgagtacgg agtgccggtg ggagtggtgg cggcgatcgt tcccaccacg aacccaacct
    64861 cgacagcgat tttcaaaatc ctcatctcac tgaaggcggg caatgggatc gtcatcagcc
    64921 cgcatccccg cgcgctgcgc tcgacatgcg agacggcgga agttctgtac cgggccgcgg
    64981 ttgaggccgg cgcgccggaa gacatcgtcc agtgcctcgg caagccgacg ctcgaaggga
    65041 cgcaggcgct gatgaaacac gagcggacgg cggtgatcct cgccaccggc ggttccggca
    65101 tggtgcgcgc tgcgtattcg agcggaaaac cggcctttgg cgtcgggccg ggcaacgtgg
    65161 ccgtgctggt ggacccgagc gcggacattc ccgacgcggt cgaacgaatc gtcgccggca
    65221 agtcgttcga ctacggcacg gtttgctcga gtgagcaggc gctcgtggcg gaggagtcgc
    65281 tccgtgaccg gatcgtcgca gaacttaagg cgcggaaggc ttacttctgt accgaggagc
    65341 agaaagagac acttggcaag ctgctgatta ccccgaactg gaccatcagc ccgaagttcg
    65401 tcgggcagtc cccgaccaag atcgcggaaa tggcgggatt cacagtaccg gccgacacgt
    65461 cgattatcgt ggcggagctt cagggtgttg gcaagcagca tccgctttcg gtggaaaagc
    65521 tctcacctgt actggcgttg tacttcgtta aggatcacgg ggctgcgctc gacacctgcg
    65581 aggcggtgct gcgattcggc gggttgggcc acacctgcgt catctacgcg aaggacgagt
    65641 ctcgcatccg ggaattcgcg cagaggatgc cggcgatgcg ggtgctggtg aacacttccg
    65701 cgccgcaggg ttccgtggga ctgacaacga acgttttccc gtcgatgacg ctgggttgcg
    65761 gcgcagccgc gggcaatatc acgagcgata acgtcggccc gcagcacctg atcaacgtca
    65821 agcgcctggc ctactccgtc cgcgagccgg acgaggcgct ggtactcccc ccgaaggtcg
    65881 cggagacgca gccggtgagc atggaccggc agtccatcgc ggcggcggtc gaacagtact
    65941 tggcggcgcg cggcatccgc ctcgaaggcg agcgtccggc tgcagcgacg cccgtaccgg
    66001 ccgctgccgt ggtcccgcag gtatcggaca tcgtggatcg tttcctcgca gcgcggcgtg
    66061 cggccggccc ggctatagcg cccgcaaagg aagcgagctg cggctgcccg gtgcatccgc
    66121 ccgcgccgcc agctcccggt gcggcgcctc cggctccccc ggctccggaa gtccagattg
    66181 tggacttcgt ttgcgagaac gacgtacgcg cggcgatgcg ggactctcgc aagatattca
    66241 tcgggccgaa gacgatcctg acgccctccg cacgcgatct tggcgagcga gagaacgtcc
    66301 tcgtcgttgc acagcgataa atgaaacaaa acctcgacac attgaagtcc gagattctcg
    66361 aacaactcgg ggcgcagggt ttcgccgtct ttcacagcta ctcgcggaac ctggaggata
    66421 cggggacgat ctattgggat gtccaccact acccagattt ccggccgttc ctcgacaccg
    66481 cgcggcagct cggcgtaaag atggtgacct tccatcatcg cgagttcacc cgggaggcag
    66541 tggaggatgc cttcgaccgc cttgaggagt gcgagatttc gccggaggag aggcgctctt
    66601 acgaacgccg gctccgcgag atgggcggtt acgaggggtt cacctgcgct gtcgagctcg
    66661 cctacgactt cgagacgcag ttgtacctgt atagtctcca aaccgcctgg tacaccgagt
    66721 ttctggacct gctcgacgaa atcgacggct acacgtccga cgaggaagcg gaggacgagg
    66781 gcccgatcgg cgggtacttc tccaagaatt gacccagcga ggatgcagcc tcaagtccgc
    66841 tggtcactcc cgcaggccga tccggagatc gtagagtctc tggcggcggc gctcggcgtg
    66901 caatttccgg tggcccgcgt cctctggcgg cgcggttacc gggaacccga agtggcgcgc
    66961 cggttcctcg acccatcgct cgccgatctg cacgatcctt atttgctgcg ggacatgcgc
    67021 ccggccgtcg aaaggctccg ccgggcgatt ctcgaaaaag aggaaatcct tctctatggc
    67081 gactacgatg tggacggcgc gatgtcggtg gtcatcctga agaaggcgat cgaactggcc
    67141 ggtggcgctg cgcgcttcca tgttccacat cgtttgcggg acggctacgg gatgcggccc
    67201 gacgtagtgg agaaggcggc gtctgccggg gtgaaactaa tcgtgagcgt ggatacgggt
    67261 atccgtgccg caggcgtggt ggtgcacgcg aacgaactgg gaatcgatgt gatcgtcacc
    67321 gaccaccatc ttccggaatc cgaattgccg ccggcctgcg cggtagttaa tcctaaccgc
    67381 acggactgcg cctatccgga gaagaacctg tgcggcgcgg gtgtcgcgtt caagcttgtg
    67441 caggctctgt tcgggacact cggctggaca gccgcgaggc agaggcggat gctcgagtcg
    67501 ttcctgaagc tggtggcgat cgcgacggta gcggacgtgg tgccgctgac aggcgagaac
    67561 cggatcctcg tgaagcacgg cctcgccgga ctgcggaccg tgcgaaacgc tggactgcgg
    67621 gcgctgctgg acgtggcggg cttcgccgac ggcgacggcc cgtccgcctc gcaggtcgca
    67681 ttccggatcg caccgcgaat gaacgcggcc ggacgtatgg ccgacgccaa cgacgtaatc
    67741 gacctgtttc tgacacagga cacgaagcgg gcgcgcgaac ttgctgatca gcttcacgcc
    67801 ctgaaccagg aaaggcagca agccgaagcc gagatcgtcc agacgattct cgaagagtgc
    67861 gagagaacgc cggtgaccgg cgaccagatg gcactggtct tcagcggtga aaactggcat
    67921 cgtggcgttg tcggaattgt tgccaacagg ctggtcgaga agttcaaccg gcccgcgttc
    67981 gtgttgagtg aggatccgga ggaggggctg gcgcatggtt caggccgcag cgtgcgtgca
    68041 tttcacctgc tggaagccct ggagtcgatg agcggccttt tcacacggtt cggtggacac
    68101 cggcaggcgg ccgggctcac cgttcctgtc gatcgcatct ccgaattccg ccggcggctc
    68161 aatgagtatg ccgccgcgcg attgacagcg gacgaccttg tgcaggaact cgatgtcgac
    68221 gccgtgctcg acctgaggga gatcagcgag aagtcggtta acgaaatcct gcggctggct
    68281 cctttcggat tcggcaatcc agcgccccta ttcgcggtgt tggacgcgga acttgcgggc
    68341 cctccggtcg tatggaagga caaacacctt cgcgtcgggc tgaaacggaa cggacgcacg
    68401 gtcatcgcga aggcctggaa cttcgtcgat cgcgcggagg agttggtcgc cggtgcgagg
    68461 ctggatgctg tgatctcgct ccaggcggat ccgtattcgc tcagccgggg ttacccaggc
    68521 tggagcgcgg agatcaagga tgtgcggcgg tcgcaggaga ggtgaactga gtcagaacct
    68581 gtcccggttt ttcccgtttg cgctcgcgca gcacctggca ggagaatgca cacatgatca
    68641 gaaccggctc gcttatcctc gccctcatca tcctggcgcc gcctgctgaa ccccagggaa
    68701 tcgaactgaa cgacggagaa gaccacggcg accccccgtt ccttctggag gagggttggc
    68761 gtccgctgtt taacgggccc gttttacagg gctggcatgg ccaggacgga aagccgcacg
    68821 agtggatgac cacgcgcgga gtgcgcatgg atcgctacgg tccgacgcgg ctgtcgccgg
    68881 ttccgacacc cggcgaccgc ctgatcaacg gcccgcaagg ccgcaccgtc aatctggtca
    68941 ccgacgagaa attcggcgac gtggagttgt acctcgagtt catgattccg aaaggctcga
    69001 actccggagt ctatctacag ggcctctacg aggtgcaggt gttcgacagt tggggttcgc
    69061 tcgagccggt gaaaagttcc gactgcggcg ggatttatca tcgctggatc gataacaagg
    69121 gcgtcggcgg ctcggctcct tcccgcaatg ccagccggcg gccgggcgag tggcaatctt
    69181 tccagatctg gttccgcgcg ccgcgcttcg acggctccgg caagaaggtg gagaacgcga
    69241 agttcatccg tgtgctgcac aacggcctct cgattcagga gaacgtagag gtggacgggc
    69301 cgacgcgagc gcacatgagc caccccgaag cgccgctcaa tcctctcatg ctccaaggcg
    69361 accatgggcc ggtggcgtac cggaatatct acatccggtc gctgcggccg atcatcgagc
    69421 ggtagcgtcg aatgcccgcg ggacgtggct ggatcgacat ctcggttccg ctccgcaccg
    69481 gaatggtgca ttggccggac gacccgcctg tccggattga gcggtttcag tcgttcgaga
    69541 acggcgacgg gtgcaacgtg tcggctcttt cgatgagcgc gcatacgggc acccacgtgg
    69601 atgctccgtt ccactttgcc gccgagggcg aaggggtcga cgtgctgccc ttttctgcga
    69661 cgatcggacc ggcgcgggtg gtcgcgatcc gtagcccgga ggcggtacgg cccgaggaac
    69721 tccgcgccca tcggattcga cgcggggagc gagtgctctt caagacaccc ggctcggcgc
    69781 gtcgattgaa gagcggcgct ttcgacgagg acttcgtcta catttcgaaa gaagcggcgc
    69841 tcttcctggc cgagcggcag gtgcgttgcg tggggattga ctacctatcg gtgggcggat
    69901 tccggaagga tctggcggag acgcacgaga tcctgctcgg agccggaatc tgggtcattg
    69961 aaggactcga cctgtcgcgc gtgaagccgg ggcgatacga attgatctgc ctcccgctgc
    70021 ggattgccgg agctgacggc gcgcccgcgc gagcggtgtt acggccggcg taacgcgggc
    70081 tgtatcagga ttcccgtttg tattgctcga tcttagggcc gtaggggcct ttcttcgtcc
    70141 tcgcgagcaa ggcgctcatc tcgtcgctgg tcatcttctg gaacgtcttg acgacgagga
    70201 cgttctcctc gagttgttcc ggagtctcag gtcctgagac gagggtatca atatcctggc
    70261 tgagcgcgaa gcgaagacac tctcgtatcg atgcgatccc gttctttgtg attgtgccca
    70321 tgccgttact cttcatcccg aggagtccaa gtcccttctt acggatgttc ggcaggacac
    70381 tctgaatgaa gctgcgatag tgcgggtcaa tcaggttgac cgggtgctga accgtttccc
    70441 agccatcgaa ggcgtcgagc aggcgttggt ggacggccgg gtcacggtgg ccggtgaagc
    70501 cgatgtggcg ggtcttcccc tgttgctttg ccttgacgaa cgcttcgagg gctccgttcg
    70561 gtccgaggat ctgatcgacc tccgcctgcg tactgacctc atgacattgc cagaggtcga
    70621 ggtagtctgt ccgcatgcgc ttcagcgtct catccagttg gcgcatggcg ctgtcgcggt
    70681 cgcgcaactg cgccttcgac atgagcagga ccttcgaacg aagaccgccg gcaagcgcct
    70741 taccgtagat ttcgtcgcta cggccgtcgt tgtaatggtg agcgctgtcg aagacggtga
    70801 cgccgagatc gacggcgcgc cggatcatgc gcacgccggt ctcttcgccg tgcatcccca
    70861 tgtgatagcc gccaaggcag aatcgcgata cgtgaagccc ggtcttgccg agagtcgcca
    70921 tgggcatcgg agcctcggcg aggaccttca accgggaacc cagagacagt gaagacaatt
    70981 caagaaaggt tcgtcgatcc attgtgcctc ctatccgagc acaggaatct gttccctcag
    71041 gacgtccgga accgctcgtc ggtttccacc tggtagccat tggctaggcg gtttgtttca
    71101 ttggccatgc cgactacggc catcaactcg gcaaacatct cctgcgtcat gcccgcctta
    71161 cgagccgaag cggtgtgtga agcgatgcag tagccgcacc cgttggtggc gctgatcgcg
    71221 acataaatca tctctttggt gagcgggtcg agcgctccgg gccgcatgat ctctttgatg
    71281 ctttcccatg tgcgcttgag cgtcgccgga tcgtgcgcga tcgccttcca gaagttgttg
    71341 atccagtccg tgttacgggt ggccatgatg tcatcgtaaa cggcccggac ttcggggctt
    71401 gcctggtcgt actcgatcag tccaaacgtt gccatgaatt cttcctccgg gtattatcgg
    71461 cgatctcact ttgcgtcgcg ccagtttgaa ccggcgccga tatccaccac cagcgggact
    71521 tccagacggt gcacgctttc catctcgtgc ttgacgagtt tcgatacttc gtcgatctcg
    71581 ctgggtgggg tttcgaagag caactcgtcg tggacctgga gcagcatcgc cgtctggagg
    71641 ccacgggacc ggagttcacg gtcgatgcgg atcatcgcaa gcttgatgag gtcggcggca
    71701 gtgccctgga gcggggtgtt cacggccgtg cgttcggcga agccgcgggc gttcgggttg
    71761 cggctggcga tgtcggggat cgggcgccgg cggccgaaga gcgtgcgcgt cactccgttc
    71821 ttgcgtactt cggcaatggt atcgtcgatg aacttcctca cgccggcata gcgctcgaaa
    71881 tagctcttga tgtagctctg cgcttcgctt tggggaatgc cgagctggcc cgagaggccg
    71941 aaggcgctca gtccgtagac tatgccgaag ttgaccgctt tggcgttgcg acgcagatcc
    72001 ggcgtcacca tcagaggcgg aactccgaac acctcggcgg ccgtgcgggt gtggatgtcc
    72061 tcaccgttgc ggaaggcctc cacgagcacc ggatcgcggg acatgtgggc gagcaggcga
    72121 agctcgatct gggaatagtc cgctgccact agaagccagc cttcgcgcgg gacgaaggcc
    72181 gcgcggatct cgcggccgag ctcggtgcgg attggaatgt tctgcaggtt cggattggag
    72241 gaagagaggc gcccagtggc ggcgcctacc tggttgaagc tcgtgtggag gcggccggtg
    72301 tccgggcgga tcaacgcagg gagcgcgtcg acgtaggtcc ccttgagctt ggcgagctgc
    72361 cggtattcga gaaccttggc agcgatgagg tgttcggcgg cgagttcttc aaggacgtcg
    72421 gctgcggtcg atggggcttt gcccttgccg taccggaccg gtacggcaag gctcaggtcc
    72481 tcgaaaagca ccttgccgag ctgttgcggg gagttgatgt tgaacggacg accggccagc
    72541 gagtggatct ccgcggtgag acgctgtatc tccgaatcca tgcggttcga gagctcgccg
    72601 agcgctgaag gatcgatgcg gatgccggtg cgctccatcc gggtgagaac ggagacgagc
    72661 ggcatgtcga tggtgtcgta cacgttccgc aagcactgcg cgtcaacctc cgctccgagg
    72721 acgccggcaa gttccagcgc gcagtcggcg cgctcttccg gtgagccgcc cagcttccgg
    72781 tcaaaacgcc gttgggcgag ctgttctagg gaacaaccgg acgggtccgc ctcgagcagg
    72841 aacgcgtaga gcatgacgtc ttcggtgaag ccgcgtgcct cgatgccgtt gcgggcaagc
    72901 tcgatcagga cggtttttgc atcgtgggcg accttcgggc gatgctcgtc ttcgagcacg
    72961 ccccgcacgc cttccaagag cgagaacggc actgcccgcc cttcgccgca tttccccgcc
    73021 agtccgagac ttgagaggga cagttcgccc tcgccagcgg cctgtactgc cactgcgacc
    73081 ggcttttcgg gcgggatcgc ggcgaggtat tcggccagtt cctcgccggt ctcaatggca
    73141 cggtagtcgc gaggctcgga atctccaccc cccacttcct tgagaagact gaagaactcc
    73201 agatccttgt agatctctcg aagggcggag tcgtccggcg gccgcgcctg aaacgtgccg
    73261 aggtcccact cgaccggtac cgttgtgtcg attgtggcga ggcgtttgct caggagcacc
    73321 tgatcacggt ggttcaacag gctctcccgg tacatcttgc gctcgacctc ggaagcgcgt
    73381 tccagcagat tctccaccga gccgaaccgg gcgatcagat ccttggcacc cttctctccg
    73441 atgcctggag cgccgggaat gttgtcgacc gcgtctcctt tgagtgcgag caagtcggct
    73501 acctgagacg gcgcgacgcc catgaattcc tcgaccttcg ccgggtcata cagcgtgtcg
    73561 tccttcgtcg ggttcagcat cgaaacccgg tcgtcgacga gctgcatcat atctttgtcg
    73621 ctcgaaacaa tgaccacgtc gacgccggca gccttcgcct ggtgggcgat ggcgccaatg
    73681 acgtcatctg cctcgaaacc ggcgtgctcg aggacgggga tgcgcattgt ttcgagcagg
    73741 cgacgaacgt aggggatctg ctcaagcagg tcaggtggcg tctcggcacg gttagcctta
    73801 tagtcggcga attcctgttc acgaaaggac gggccgctct caaagatcgc cgcgatgtag
    73861 tcgggatgat gtgcggagat cagcttccgg agcatgttgt ggaagatgta aacggcctcc
    73921 gtggagaggc ccgagcgggt gcgcatgggc ggagctccgc ttcgggctcg ggcgtggtaa
    73981 gcacggaaaa tgaagccgaa agcatcgatg aggaacaggc gcgggcggga catgcctccc
    74041 catgataccc ccagtgccgt gtgggaggca cagcacacca caacatgctg caatgtgtgg
    74101 ttattttgca acagttagcg ttgcgtaatc cattgaaact aaagggtatg tgagtggtgg
    74161 gtcaattgcc ggtatacgta gcctagtaat acctgagcaa tctagaggat agtactgctt
    74221 gcgcttcgaa accacgatac aacgaccggt cgccgcgtct ggagtggggt tacacagcgg
    74281 cgtaccggta tcgatccgga tcattccagc gccgccgtcg accggcatcg tgttcctccg
    74341 cacggacctc gaacggtttc cgatacccgc gaactggcgc aatgtcgcca gggtgagcta
    74401 tgccacgagc ttgatgcggc aaggcgtgct gatctcgacg actgagcatc tgctcagtgt
    74461 cttctacagc atgggcgtgg acaacgctta tgtggagatc gacaacctcg aggtcccgat
    74521 cctcgacggc agcggccagc cattcgtgaa gatgatcgag cgtgcgggcc tccgtatgta
    74581 ccggcggcgg cgaaggtacc tgcgcatccg ccggccggtg acggtcgaag accgggacaa
    74641 gcggatctcg atcttgccgg ctgaccgctt cttgctgagc tgcgagattc agttcgacca
    74701 tccgctggta gggcagcagt cggtggagat ggaggtgacg ccagggcgtt acgcgagtga
    74761 gctcgcgccg gcgcgtacgt tcggtttttc gcacgaactg gaccaaatga ggaacatggg
    74821 tctgattcgc ggggcgtcgc tcgacaacgc cgtttgtttc gaacgcgaaa gcgttatgaa
    74881 tccaggtggc ttgcgattcc ctgacgagtg ctgccggcat aaggcgctgg acttgatcgg
    74941 tgatctggcg ctgatcggca agccactgct cggacacgtc atcgcggagc gggccggtca
    75001 cgccatgcac gcggcgcttg tggcacgcat catgtccgac ccgtccctgt acgaagtgct
    75061 gagcttcgat caactcgccg acagggtggc gcacgcactg gtatcctgaa gggctgtgtc
    75121 gtttgcgcgc gcggcccggc aactcccgaa cttcatcacg gcgacacgaa tcgttcttac
    75181 gccgtttatc gccgtatcga tcctcggtgg cggccaccga aatgcgttga tcttactggt
    75241 catcgccggt attacggacg gccttgacgg ctatctcgcg cggcgcttcc agtggttctc
    75301 caggctggga gcgtacctcg atccagtggc agataaactc ttactggccg ttgtttacat
    75361 ctgtttgggt gtgatcggac tagcgccgct gtggctggtc gccgtcattt tcgggcgtga
    75421 catcctgatc cttgcattcg gcgggacgat gatggcaagg ggaggccgcc gggaactccg
    75481 gccgtcgatc tggggaaaga tgagcactac ttttcagatt gccaccgggg tggccctcat
    75541 cgccggccgg gcgtaccctg cgctcgcgtt ggaccgggcg ggcctttggc tgatccctgt
    75601 cacggctgcc gccacgctgt ggagtggatt gcactacgcc tggaccgcct ggcggatggt
    75661 acgcggtcct gcgtagtagg attgacggag caggcctgcc agagtaggct aagggatggg
    75721 ggccgccatg acacgctatc taccctcccg gcgctatcta tggggcgggc tcgccgcctg
    75781 gggactggcg gcattgtcgg gtgtgttcgc gatcgagtgg gcgccggcgc tgatcgccgt
    75841 cgtactcttt cttatcagtt cggggctgct tgtattcctc gcgttgcggc ctccggtgga
    75901 aatccgggaa agccacctga ttgtgggcaa ccgtgccatt ccttggggcg aaatccgccg
    75961 tgtggatcgt accgggtgga tatctccgtt ggtggtccgg ctcacgtttt ccgatgacag
    76021 ccggatgctg ttgatctatc cgggagatct cgactccgcg aacagcttgc tccggcattt
    76081 gcgcagacac tcgcgcgagg cgcagatcga cggcagggcc catcgggagt tctggggcga
    76141 cgctcttccg gccgtgcccg agaggaaaga agaactgcca tccccccgct acaggctact
    76201 ccgtcccgag gacgaagcag aagtagagcg catgttccat caactgaaaa cggtgggacg
    76261 actcgaccag aataactctt cggacgagaa gtagcccttg tacttctcga aaccctttcg
    76321 ctcaggtttg gctgtcggcg gcgctctcgt ggcggttctg gcaaccacat atcccatctc
    76381 actgggggcc ctcggcgggt ttctggtaaa ggcggagcag ccagtgcggg ccgacatcgc
    76441 ggtggtgctc gccggggact acttcgggaa ccgcatcaca aaggcgggcg agctggtccg
    76501 tcagggttac gtttcgaaag tgcttgtcag cggtccgaag ggattctatg gctacgccga
    76561 gtccgacttg gcggtaccgt tcgtggtcaa gaaaggatat cccgccgagt gcttcattcc
    76621 gtttcccctt gacgccgatt ccactcggga agaggcggaa atcatcgtcg gggagttgcg
    76681 acgccggaac gtgcatacgt ttttgatcgt gacgagcgat taccactcgc gccgtgccgg
    76741 ctcgatcttt cggaatctgg ccggcggcct gaagtttcgc gtagtcgctg ctccagataa
    76801 gtcgttccgt gccgaggggt ggtggaaaac caggccggga caaaagcagt ttgcgcttga
    76861 atggatgaag acgatcgcga cgtggatcgg tctgtaacaa catgagagag gccctgaaga
    76921 ccgtctggcc atacatgtgg cgctaccggc gcggttgggc gcggggaatg ggcgcgctgt
    76981 tgctaaaaga tctttttgcc gccgctgttc cattctttat cggaaagggg gtcgattcga
    77041 taacccgcgg cttccggctc ggcggtattc tgtggttcgc ggcagcgctc gtcggttttt
    77101 ccgccatcaa agggattttc caatactgga tgcgagtgat cctgatcggc atctcgcgcg
    77161 acatagaata cgacctcaga aacgatctgt tccagaacct ggtccggctt tcctcggatt
    77221 tctatgcgag ggtgcgcacg ggcgatatca tggcccgcgc gacgaatgac ttgaacgccg
    77281 tccggatgat gcttggcccc ggcgtcatgt actggtgcga aacgtgcctg acctttctgg
    77341 ttgtgaccgc catcatgctg gcggtggatt ggccgctgac gctgcttgcg ctgctgccgg
    77401 cgccgctgat cagcctcgcg gtggtgtatt tcggccggaa gatccacgcc aggttcgagc
    77461 gtatccagcg gatgttctcc gatattagca gccgggtgca agagaacatc tcgggcgtcc
    77521 gcgtgatccg ggcttacgtg caggaagagg cggaactccg gcaattcgag aagctcaaca
    77581 aggagtacat agcggaaaac ctcaagctcg cccgtatctc cggactgttc atgccgctgc
    77641 tccaagcgct ggtcggactc acgctgcttc tggttctttg gatcggagga cggcgaatcc
    77701 tcgccgggca catcacgctg ggacaattcg tgatgttcaa tacgtacctg gctatgctcg
    77761 tgtggccgat gatcgccttt ggatgggttg tgaacctgat gcagcgcggc acggcttcgc
    77821 tgaagcggat caacgagctt ctgacggagc ggccgacgat tgccgagccg gcatcgccaa
    77881 acccgctgcc ggaggcgcct cccggtgaga tcgaattccg cgacgttaca gtggagtatc
    77941 aaaccggccg cgcgctgagt ggcgtgagcc tccggattcc ggctggcgcc accgtcgcca
    78001 tcgtcgggca cacgggtagc ggcaagagca ctcttgtcaa cttgattccg aggctgcagg
    78061 atccgacacg cggcgcggta ttgctcgacg gaaccgatct gcgggagctt tctccggcgg
    78121 acttgcggcg ccagatcggg tttgtccctc aggaaacatt tctcttcagc gcgacgctgg
    78181 cggagaacat cgccttcggc gtggagacgg cgaccccgga gcagatcgag cgggcggcgg
    78241 agatcgctgg tctaggtccc gatattctct cgttcccgga cggttaccag accattgtgg
    78301 gcgagcgcgg catcacgttg tccggtggcc agaagcagcg gacggcgatc gcgcgggcgg
    78361 tactccgcaa tcctcgcatc ctgattctgg acgacgcgct ttcgagcgtg gacacgctca
    78421 ccgaggatcg catcctcagc ggtctggcgg aggtcatgcg cgggcggacg accattctga
    78481 tttcccaccg cgtctcgacc gttcgcagcg cggatcggat cttcgtcgtc gagcacggcg
    78541 aaattgccga ggagggcacg cacgccgaac tgctctccct cggcggttac tacgccgatc
    78601 tacatcagaa gcaactcctc gaagaggaac tggaagcgat ctagccagcc tgcggcgccc
    78661 ggcctctccg gcagataatg tcacggaaag gagattccca tgcacttctt gcgactgctg
    78721 ccggcactgg cagccacact gatttcatgc acgcccacag ccgccttcgg gcaacagatg
    78781 accacgccgg agggcgtgag catactccgg aacaagagtt tcgacgactc cgaccgcgcg
    78841 cggaaggaga ttctcgacct gtaccgggat ctgcgcgtca ccgacgtcct cgacggcatg
    78901 gacttgatcg gcttgcagga catcgggctc atggataacg aaatccgtcc attgtggcgg
    78961 gataccgaca gcttcacgca cagattcgtg ggccttgcgg tcacggtgcg gcacgtgccg
    79021 agcgatgtcc gagtgggaca gaactcgttc cccgatcttg cgggcttcct gaaattcaag
    79081 gatgaacagt acgggcgtgc gccggatgcc tggctgaaaa ccgcacgcca aggcgatgcc
    79141 gccgtgatcg acgcgagcgg cgtcttcgag actgggttca tcggctcaaa caacagcctt
    79201 gggtgggccg agaagggtgt cgttggagta gtcacgaacg caggggcgcg agacaccgac
    79261 gagatcgtga aggtcaagcg gataccggtc tattgcagga acggctacag cacgagaggg
    79321 gtccgtccgg gacggctcat cgcggagtcc tacaactttc cggtcaactg cgcgggagtc
    79381 acggtattcc ccggcgacgt tattgtcgcg gacggcgacg gcgtcatcgt cgtgcctcgg
    79441 caggcggcga tccaagttgg caaacttgcc cgcgagatca atgtggacga cgagcagggc
    79501 cgtggcaaac ggttcgagcg gctgggtatc cctcatgaca gcacggtcaa ggtaaagtaa
    79561 gaagaccccc tgggagcgtg cgtaaaccgc ctctggggca atcttaccaa tgccgtacaa
    79621 tactctcgcg atttgtgtag ccgatgagct gatcttcggc cacagcaagc agccggtgcg
    79681 ctgcggattc gatctgac
//
LOCUS       DATIFQ010000344         4915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_306945, whole genome shotgun sequence.
ACCESSION   DATIFQ010000344 DATIFQ010000000
VERSION     DATIFQ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306945"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2352)
                     /locus_tag="VLE22_16440"
     CDS             complement(<1..2352)
                     /locus_tag="VLE22_16440"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB16046.1"
                     /translation="MQLAPKAYAVLSYLIENPGRIVSKEELLDAVWSDTFVQEAVLKS
                     CVLEIRKALGENSKDPRHIKTVHRRGYTFVDLAAESPARLPTTGLLGRAADLQRLHSL
                     LEKAGSGNRQIAFVTGEPGIGKSTLIEHFVKQAATHTLRGECIEHFGAHEAYYPLLDA
                     LGRAMKNASIPGLPDALRTYAPTWLREMPGSVSPEDREALNIEALGATRDRMLREITE
                     AIEALATREVLLLVLEDLQWSDLSTLDVISRIAHRVEYTRLLVIASYRPVEVILNNHP
                     VRSLKRDLLARDRCVEFELELLNKSDVGALLNARFPGHSFPGEFADLVHTRTEGNPLF
                     VLNVLDYAVSRNFIARAGNHWRLNAPLQDFDLGMPESLAQALANQVERLTSQEQAVLE
                     NSSVAGVAFPVSLIVSGPEEQLSCETCCDALAARDLFIQAAGLAAFGSLPATPQYRFT
                     HALYRDFFYKRLSPARRVRLHQTIAERLERIFDGAVRDVAPELALHFEQGRCVEKAIF
                     YLRLVAERRARRHALQESLDALERALNLAATLPDPGRTRAELELNQQLGLVYRLMGQL
                     PASLAAFEKMFDLAVRADLREFQLHAQCWLASVASWVDRDRCLRAVDTIVSLCSSGVD
                     ADLYANAMAQVAYWNMHFRGWDERDARASAAGLEVARSSGDKMLLALHATRHSYFQVL
                     SSRYREACETAQEGVRIASEFDSLLDYSVGHFFEASALLFLGEWGQLERLLHSAMDMV
                     RKNGHETWEVLFGLMEAYLWIEAFSFEPARSACEAYLKRSRALG"
     gene            2525..2716
                     /locus_tag="VLE22_16445"
     CDS             2525..2716
                     /locus_tag="VLE22_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16047.1"
                     /translation="MHRAFVVQVTAECDPPDTFGGRVEHVHSGQAIHFENVSQFLNFL
                     ASSLRSERQIEEEERRDAG"
     gene            2922..3668
                     /locus_tag="VLE22_16450"
     CDS             2922..3668
                     /locus_tag="VLE22_16450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB16048.1"
                     /translation="MNAVTIAPEQTGSLSSVVSIVHDLRNPLATIHGSAEMLVRSNLS
                     QPQVHRIARNMYCASVRMRELLEEFLGRSRGTEKKVEPSDVRELVTSAVDKIAVSAEL
                     QLVQIVQVVPEGLLITLDRHRMRRVLLNLLVNALEVMPNGGTIHISAVSDSRSVWIRV
                     RDTGPGIPPEIRGRLFQPFATARKVSGIGLGLASSRQAVIDHGGQIWAESSRKGACFA
                     VRLPRTVPQPPSHRNSVCPKMRRSGADMQI"
     gene            3794..>4915
                     /locus_tag="VLE22_16455"
     CDS             3794..>4915
                     /locus_tag="VLE22_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16049.1"
                     /translation="MSSKTTPMTQNGGLLALASLALAALTATGLGAQTAGPYGADREP
                     NFHSLRKRGDIKFLPAPLQDRLVELARRPHSYLPLTAFSEADEPSQLFGYYLLDTTTI
                     PPNVFTSIVPGINDNGVIPTGANFANGGLPTVGAVRVTLEPKPGLPTDPNDPRAFIDM
                     FTDISGLFVINNESGWYEGWMTWDLRVPRVAEPLADGRAQYGTMTPADAEVLAARGTG
                     NNVPGHFFTLDGNAVRFPSASAGGPIPGNTIGFSVSIGAFNAQQQSDVHAYWEFNRGT
                     NWVFPLYELPSTGGLPGTFPALQYSVQSVIPGSGPFGVRNTAATHGDDPNNPRDPDRF
                     EPVSSGQTETRNRFVPSGLAAEIFRDVFTRPASFLPGVTD"
BASE COUNT         1022 a         1506 c         1439 g          948 t
ORIGIN      
        1 gcccagcgcg cgcgacctct tcagatacgc ctcacaggcg ctccttgccg gttcaaacga
       61 aaacgcctcg atccataggt acgcttccat tagaccgaat agaacttccc aggtctcgtg
      121 gccattcttc ctgaccatat ccatggccga atgcagcaga cgctccaact ggccccattc
      181 gcccaagaaa agcagcgccg acgcttcaaa gaagtggccc acggagtaat ccaacaggct
      241 atcgaactcg cttgcgatgc gaacgccctc ctgggcggtc tcacatgctt cccgatagcg
      301 tgacgagagc acctggaagt acgaatgccg tgtggcatgc agggccaaca acattttgtc
      361 gccgctactt cgcgccactt ccagtccggc tgctgaggcg cgagcgtcac gctcatccca
      421 gccgcggaaa tgcatattcc agtaggcgac ctgcgccata gcgttcgcgt aaaggtccgc
      481 atccactccc gaactgcaga gcgagacaat ggtgtcgacg gcgcgcaggc agcggtcgcg
      541 gtctacccag gaggccacgc tggccagcca gcactgggcg tgaagctgga actctcgcag
      601 atctgctcga accgcgaggt cgaacatctt ttcgaacgct gccagcgatg caggcagttg
      661 acccatgaga cgatacacta gaccgagttg ctggtttaac tcgagttccg ctctagtccg
      721 accagggtct ggcagcgtcg cagccaagtt cagcgctcgc tccagcgcat ccagggactc
      781 ttgcagcgcg tgccgcctgg ctcggcgttc agcaaccagg cgcagataga agatcgcctt
      841 ctccacgcac ctcccctgct caaaatgcag tgccagctcg ggcgccacgt cccggacggc
      901 gccatcgaat atgcgttcaa gacgttcggc gatggtctgg tgcaggcgga cgcggcgtgc
      961 gggagagagc cgtttataga aaaagtctcg atacagggcg tgcgtgaacc gatattgcgg
     1021 agtggccgga agagagccga acgccgccag cccggcggcc tggataaaca ggtctcgggc
     1081 cgccagagcg tcgcagcaag tctcacatga gagctgttcc tccgggccgg acacaatcag
     1141 ggaaaccgga aacgcgacac cggccacgct ggagttctcc agaacggcct gctcctgcga
     1201 ggttagccgc tcgacttggt tggcgagcgc ttgggcgagg ctctccggca tgccaagatc
     1261 gaaatcctgc agaggagcgt tcaaacgcca gtgatttccc gcccttgcaa tgaagttacg
     1321 cgaaaccgca taatccagca cgttgaggac aaacagagga tttccttcgg ttcgcgtatg
     1381 caccagatcc gcaaactcgc cagggaagga gtggccggga aagcgagcgt tcagcaacgc
     1441 cccgacatcc gatttgttca gcaattcgag ctcgaattca acgcagcggt cgcgggcgag
     1501 caggtctcgc ttcagcgaac gcacaggatg gttgttgaga atcacttcca cagggcgata
     1561 actggcgatc accagcagcc gtgtgtattc cacgcgatgt gcgatccgcg agatgacgtc
     1621 cagcgtggaa aggtcgctcc actgcaggtc ctcgagaacc aggagcagca cctcgcgcgt
     1681 ggcgagggct tcgatggcct cggtgatttc gcgaagcatg cggtcgcgtg tagcgcctag
     1741 ggcttcgata ttcagtgcct cccgatcctc cggcgatact gagccgggca tctctcgcag
     1801 ccaggtaggc gcgtaggttc gaagagcgtc gggcaggccc gggatggagg cattcttcat
     1861 ggccctcccc agcgcgtcga gaaggggata gtacgcttcg tgcgctccga agtgttcgat
     1921 gcactcgcca cgcagcgtat gcgtcgcggc ttgctttacg aagtgttcga ttaatgtcga
     1981 ttttccgatc cccggttcac cggtaacgaa tgcgatctgg cggttgccgc tgcccgcctt
     2041 ttccagcagt gaatgcagcc gctgcaaatc cgctgcgcgg ccgagtagac ctgtggtcgg
     2101 aagcctcgcc ggtgactcgg ccgccagatc gacaaacgtg taaccgcgcc gatgcacggt
     2161 tttaatatgc ctgggatctt tcgaattttc ccccaaggct ttccgaatct cgagcacgca
     2221 cgatttgagc accgcctcct gcacaaacgt gtcggaccaa acggcgtcca aaagttcttc
     2281 cttgcttacg attcgaccag gattctcaat gagataggac aacacagcgt aagccttcgg
     2341 agcgagttgc acgcgcaacc cactacgcaa gacgcactgg tttgcgaggt ccaacgtaaa
     2401 tgaggagaac cgctttaaca acgccagccc ccttcttgac caagcgctgt gtcaaaagga
     2461 acaacttctt gactgtttcc ttacgacttc cagatctctt cattctactc tgtattcaga
     2521 gacgatgcat cgcgctttcg ttgtgcaggt taccgcggaa tgtgatcccc ccgacacctt
     2581 cggagggcgg gtggagcacg ttcattccgg gcaggcgatt cattttgaaa acgtgagcca
     2641 gttcttgaac ttcctcgcca gttccctacg gagcgaaagg cagatcgaag aggaagagcg
     2701 ccgggacgcc ggataggaat tccaggacct cgggacccgt ttgaaagtca gggaggcgag
     2761 ttaaatgaag agatattgga tttcgtggac gacgaccgcc cccgtttgcc tggctttgct
     2821 cttggctggg aaccaagggt ttggctttaa cgttagcctc atttcggttt cccacgcttc
     2881 gccgagagct gccaaagtgt gccaagaagg aaagggacaa aatgaacgca gtaaccatcg
     2941 cccctgaaca aacaggttcg ttatccagtg ttgtgtcaat cgtccacgat ctgcgcaatc
     3001 cattggccac aatccacggc agtgcggaga tgctcgtccg ctcgaacctt tcccaaccgc
     3061 aggtccatcg catcgcccgc aatatgtatt gcgcctcagt ccgcatgcgt gagttgctgg
     3121 aggagttctt gggccggagc agaggtacgg agaagaaagt ggaaccttcc gatgttcgtg
     3181 agttggtcac aagcgccgtg gacaagattg cggtcagtgc tgaactgcaa ttggtacaaa
     3241 tcgtccaagt cgtacctgaa ggcttgctca tcaccctgga tcggcatcgc atgcgtcgcg
     3301 tgctgctgaa tttgcttgtg aatgcgttgg aggtcatgcc caacggggga accatccaca
     3361 tatcggcggt ttccgacagc cgttcggtat ggatccgggt tcgcgatacc gggccgggga
     3421 tccctcccga aattcggggc cgactcttcc aaccgttcgc aaccgcgcgc aaggtgagtg
     3481 gcattggcct gggccttgct tcttcacggc aggctgtgat cgatcacggc ggccagatat
     3541 gggcggagtc gtcccgcaaa ggagcctgtt ttgcggttcg cctgcccagg acggtcccac
     3601 agccaccgtc tcaccgtaat agcgtttgcc cgaaaatgcg ccgaagcggc gcagatatgc
     3661 agatatgagg agcgattccc tcgggagccc tccttaatca atcccgcaga ggctgctcgc
     3721 gccttgagcg tgtcggcagc ctttgtttcg aaacttcaac taacacacat aaccaagaca
     3781 tcaaggagac aacatgagtt cgaagacaac acccatgaca cagaacggag ggctgctggc
     3841 actcgccagc ctggccctgg ccgcactaac ggcgaccggc cttggagcgc agacggccgg
     3901 accctatggg gccgatcgcg agccgaactt ccactcgctc cggaaacggg gcgacatcaa
     3961 gttcctgccc gcacctctgc aggatcggct agtggagctg gctcggcgac cacattcgta
     4021 cctgcctctc acggccttca gcgaggcaga cgagcccagc cagctattcg ggtactacct
     4081 cctcgacacc acgacaatcc caccgaacgt gttcacgagt atcgttcctg gcatcaatga
     4141 caatggtgtg atcccgacgg gcgccaactt cgccaacggc ggacttccga cggtgggggc
     4201 ggtgcgcgtg acgctggagc cgaagcccgg cctcccgacg gatcccaacg atccgcgcgc
     4261 gttcatcgac atgttcaccg acatctcggg cctgttcgtc atcaacaacg agtcgggctg
     4321 gtacgaagga tggatgactt gggacctgcg cgtgccacgc gtcgcggagc cgctcgccga
     4381 cggccgcgcg cagtacggga cgatgacacc ggccgacgcg gaggttctgg ccgccagggg
     4441 aaccggtaac aatgtgccgg gccacttctt cactttggac ggcaacgcgg tgcgctttcc
     4501 gagcgccagc gccggcggtc cgatcccggg gaacacgatc ggcttttcgg tgagcatcgg
     4561 tgcgttcaat gctcagcaac agagtgacgt gcacgcgtat tgggagttca accgaggcac
     4621 gaactgggta ttcccgctct acgagctgcc gtccactggt ggattgcccg gcacattccc
     4681 ggccctgcag tactcagtgc agtcggtgat tcccggatcg ggccccttcg gcgtgcgcaa
     4741 cacggcggcc acccacggtg acgaccccaa caatcctcgc gatccggacc gtttcgagcc
     4801 ggtcagctcg ggacagaccg agacgcgcaa tcgcttcgtc cccagcgggc tggccgccga
     4861 gattttccgg gacgttttca cgcggcccgc ctcgttcttg cccggcgtaa cggat
//
LOCUS       DATIFQ010000345        15036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1073718, whole genome shotgun sequence.
ACCESSION   DATIFQ010000345 DATIFQ010000000
VERSION     DATIFQ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15036
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073718"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..758
                     /locus_tag="VLE22_16460"
     CDS             105..758
                     /locus_tag="VLE22_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16050.1"
                     /translation="MTYFWSVPIWLGIVLQILVIAALLRGPYRSFKVLFVYSVVLFLI
                     TVGEAAAFYDREIWTKTSRHYWIGDAILQTLIFVFVISLIHRAMATSLKRATIRRILV
                     CGAALFVTSSVYLTHEPKFGAWMTTLSRNLGLAAVILNLVLWAALIREPSADRKLLLV
                     SGGLGIQMAGKAIGHSLRQLSRSMIFTGDLIIVMSHLACLYIWWQAFRSFDRPAPER"
     gene            868..2232
                     /locus_tag="VLE22_16465"
     CDS             868..2232
                     /locus_tag="VLE22_16465"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB16051.1"
                     /translation="MSLLKDSLNRRDFVRSAAGVSATLGMAGQALASRTRSQSGRVLG
                     ANDKINVGVIGVGGRGAYLAREFAEIGEKTGACKVAAVCDVWQKRVTQQKEKHQCDGY
                     LDYHEVINRKDIDAVVIATPDHWHARIALEAMDHGKDVYLEKPMCHTVEEARQLVSTV
                     RETKRVLQVGSQTTSGDQWNKARKAIADGMIGQMIMSQGSYHRNSKEGEWNWKIDPEA
                     GPEGKGDNFLDWKMWIGPAQKRSWDPDRYFRFRKYWDYSGGIATDLFYHVVAPMNICW
                     GEPQFPYKVMAGGGIYVFKNRPENPSLPDREVPDTFHLIGEYAKGHSIVLSSSMANSQ
                     HIPGLIRGHEGSIIMVEHGQFESLTPFISVIPEKRVVNDEYKAKFGDEPIRIPVEETE
                     TITVHITNFLDCMRSRQKPTLDVETGARAQVLISMAVQSYREGRILYFDERNWKVVAK
                     PPRA"
     gene            2295..2789
                     /locus_tag="VLE22_16470"
     CDS             2295..2789
                     /locus_tag="VLE22_16470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB16052.1"
                     /translation="MNYELADLVEDDRTAIVSIFNHYVEHSFAAYPDEPVTDTFFDRI
                     LEMSKGYPAVSIKAETGEVVGFGFLHAFHPATTFRRTAEITYFLLPVHTGKGLGNRLL
                     AYFIEKARDMEIDNLLASVSSLNEQSLRFHARAGFERCGTFRAVGRKLDREFDVVWFQ
                     KRLS"
     gene            complement(2852..4636)
                     /gene="aspS"
                     /locus_tag="VLE22_16475"
     CDS             complement(2852..4636)
                     /gene="aspS"
                     /locus_tag="VLE22_16475"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HSB16053.1"
                     /translation="MPESVPLDFLGELRRTHTCGQLRASDAGSRVLLMGWVHRRRDLG
                     GVIFIHLRDRDGVTQAVFHAGTESKVHEKAELLRSEYVIAVEGILQKRTPETVNSAIE
                     TGEVEVVAEKLWILNESRTPPFPMEETVDVSEDVRLKYRYVDLRRPHMQRNIILRSKI
                     SFAVREALNSMGFLEIETPFMTRSTPEGARDYLVPSRVQRGHFYALPQSPQIFKQLLM
                     VSGFEKYFQIVRCFRDEDLRADRQPEFTQIDLEMSFPQEEQIFEVIEPLVRRVCAVAG
                     HDVRFPFTRLTYRQAMRSYGIDKPDLRLPPFHAVEDLFPGANLTTEGLPLVAIRIPKT
                     GVLSRKERDELKAFGTERGLRVYDDVKRLERDFPEPIAAVRERTGAAEDDLLLLAGWP
                     GAPKGQRPIDAVFMACGQLRLYAAQKYNDRHGLLDPKNFQFLWVVDFPMFEWNEDEKR
                     WDAAHHPFTSVMDEDIDKLTTDPANCRAKSYDLVLNGVELGSGSIRIHRRDVQSKVFE
                     ALGFSDEEARRRFGFLLDALEYGAPPHGGIALGLDRLVMILAGETSIRDVIPFPKTAR
                     GTDLMCDAPSLVPEHQLRELGIKVVGGA"
     gene            complement(4652..5893)
                     /gene="hisS"
                     /locus_tag="VLE22_16480"
     CDS             complement(4652..5893)
                     /gene="hisS"
                     /locus_tag="VLE22_16480"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSB16054.1"
                     /translation="MIRAVKGTRDILPPSSGVWNDVEHRARDVFRAYNYHEIRTPIFE
                     ETLLFARGVGEETDIVTKEMYTFEDKDGSSLTLRPECTASVIRAYIEHRLDQQPGTMK
                     LYYMGPMFRRERPQKGRYRQFYQIGAEAIGSESPAVDAEVIELAVEILSRAGLAGFRL
                     LLNSVGCSECRPKFLAALREELRKVSSSLCADCRRRAETNPLRVLDCKVEADQPVIES
                     LPSILDYLCVACREHFETVRGYLQDREIAFEVRPRLVRGLDYYMRTTFEITHGALGAQ
                     NSVLGGGRYDGLAEALGSKVHSPGIGFSIGEDRLVLTLEESNTGAGTRALDLFVVPLG
                     EAAFRKVAPLARRLRLSGKSVEVVADARLKRALELANKTGARFALIVGDDEIAAGTFQ
                     LKDMTSGGRQTVNESELFERL"
     gene            6056..8101
                     /locus_tag="VLE22_16485"
     CDS             6056..8101
                     /locus_tag="VLE22_16485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HSB16055.1"
                     /translation="MFRAFIIFLALTASTQAQKLPFDVDALLKIARIGDPQISPDGTS
                     VAFTAQRVDFDKNTLATHIYVVPVAGGTPRQITSAGSRNERPRWAPDSKTIAFVSDRG
                     GTGQVWLMDPDGAHARQVTNLSTEAGDVVFSPNGKQVFFTSNVYPECVDDACNKKRLE
                     AERASKVKARIYTSLLYRHWNEWRDARRKHVLVADLTGGAVRDLTPGTRDVPPFSLGG
                     PDDFAVSPDGEELCYVMNPDPEPATSTNSELYVVPASGGEATKITVNVGADASPRYSP
                     DGKHIAYRAQVRGGYESDRWRLVAMQRDTGESVILTESLDRSVSSFTWSPDSTRLFFT
                     VEDRGRKVVQMIPVTGGGIRVIASGASQIDDVQLSADGKVMIYAEQRASRPAEIFRAS
                     STGGAAVPLTRLNDLHLGKYQLTAAEEFWVDGADGAHVHNFLVKPPGFVEGKKYPVLF
                     LIHGGPQGAWGEDWTYRWNAQVFAGAGYVVVMPNPRGSTGYGEKFTDEISGDWGGKVY
                     DDLMAVVDYVAKQPYADAGRMAAGGGSYGGYMVNWILGHSNRFKALVSHAGVYDLRSM
                     FGETEELWFPLWDFPGAPWDNPETYSRWSPSYFAKVFRTPTLVIHGELDYRVPVGQGL
                     QLFTALQLRKAPSKLLLFPDEGHWVNKPQNSALWYKTFIDWIDTWAKNAGTSGDKSE"
     gene            8059..9261
                     /locus_tag="VLE22_16490"
     CDS             8059..9261
                     /locus_tag="VLE22_16490"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HSB16056.1"
                     /translation="MGQERRNIGRQVRIALLLSLLATAAIAAETSQEQFDRLMKDVLI
                     ADLHVDTPMYVVDEGYRLGEEHAYCEYDIPRMRRGGLGAVFFGIYVQPQDYPPSVWMT
                     RVLESIDAVHEEARRNAKDIEVAYTAADIERIRKAGKIAVLLSLEGGHLIQDSLAVLR
                     NFHRLGIRYMTLTHFRTNNWADSGTDAAVHNGLSPLGREVVREMNRLGMMIDVSHVSD
                     KTFYDVLSASRVPVIASHSCARAICDIPRNMDDDMLRAMKKNGGVVFVNFSIAYLDKK
                     AYDVFISYRDERDREIAETLGAHAGSPLRWELKRAIQQRYRARLPKVDVKAALRHIDH
                     IAKVAGPDHVGIGSDFDGISGMVPAGLEDVSKFPALIRGMIDLGYSDDDIRKIAGGNL
                     LRVMRANE"
     gene            9366..9830
                     /locus_tag="VLE22_16495"
     CDS             9366..9830
                     /locus_tag="VLE22_16495"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB16057.1"
                     /translation="MRTFISRILERYESGGLSRRDLIQALASLAAAAPAASAARSTFK
                     GVGLNHIAVRVTDIPRSKAFYQGLLGLPLMSESSGSCFLKLGDEFLTLFQNRNPGLDH
                     YCVEIEAFRPDEVMDQLKRQGLQPRRPDGTDRIYFRDPDGLEVQLAAVGHQA"
     gene            complement(9855..11174)
                     /locus_tag="VLE22_16500"
     CDS             complement(9855..11174)
                     /locus_tag="VLE22_16500"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB16058.1"
                     /translation="MSTGQDGLPRKLGLLDATSIVIGTMVGAGIFIVPGSIAREIPSS
                     AAILATWTACGIISFFGALAYAELGAMMPDSGGQYVYLREAYGELTAFLCGWSAFLIV
                     QSGSIAAVSVGFGIYASYLLPQAPGYLWAPAAIAAFSFINYRGVKAGARTQNLFTFLK
                     VAGLVVLIASALLRRPPALAGGSFAWGSLSLDGIVVAMLGCFMAYDGWQYVSFVAGEV
                     REPQRNLPLSLALGTAAVMLLYLLANLAYMHVLPLGAIAATERVAAEAAAKTMGPAGA
                     IVIALTIMLSSAGAANGAILTSPRLYFAQARDGLFFRRLGEVHPRFRTPSFSIAAQSV
                     WAIVLALSGSYETLLSYVLFAMWLFHALTVFAVIVLRRRHPERPRPYRIFGYPFTPLL
                     FTIFAFGFVVNTFTTRPGSSLVGAVLIAAGIPMYWIWNRGRLRDRSG"
     gene            complement(11247..13364)
                     /locus_tag="VLE22_16505"
     CDS             complement(11247..13364)
                     /locus_tag="VLE22_16505"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSB16059.1"
                     /translation="MRTDDLSRRDFLSVAESGLFVFFPLEPLAAFQEPARLPARQGYP
                     TDFNAYLRIDAGGWVTCLVGKVELGQGSKTALAQILAEELDVALDSVDMVMGDTDICP
                     WDMGTFGSLSIRQFGPILRGAGAEARAVLLQMAAEHLQVEAGRLRVKAGVVADPSAPG
                     KRVTYAQLVQGKRIERHIEKAPVKPVAAYTVVGQSPRRKDAIEKVTGQARYAGDMAIP
                     EMLHARILRPPAHGATLKDVDTSAAERVPGVRVVKEGDFVAVLHERRDLADQALGLMR
                     AQFELPRPGVDDQAIFDHLLKTAPAGQVLAESGSLSEGERRAAAIVDETYLNSYVAHA
                     AMETHSATATIEDGKATVWASTQTPFPVRQQVAQALGFTPQNVRVITPYVGGGFGGKS
                     SSRQAVEAARLAKFTGKPVQVVWDRAEEFFYDTFRPAAVVKIRSGLTGDGRISFWDYS
                     VYCAGDREAKQFYDIPHQRTVSSGGWGGGNPAGLHPFSVGPWRAPSVNTNTFARESHM
                     DILAAKAGIDPLEFRLSHLTDKRMQRVLEAAAKRFDWTSGRAPSGRGVGVACAIYLGT
                     YVATMAEVAVDRSTGRVEVRRVVSAQDQGVIVNPDGSRQQMEGSITMGLGYTLTEEVH
                     FKNGEVLDHNFDSYQIPRFSWLPKIETVLIENPEVPASGCGEPPIVTMGAVVANAVHD
                     AVGARLFQLPMTPARILEALRRG"
     gene            complement(13361..13840)
                     /locus_tag="VLE22_16510"
     CDS             complement(13361..13840)
                     /locus_tag="VLE22_16510"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSB16060.1"
                     /translation="MQRTVSFTLNGKPTRVTVDDERMLLWVLRNDLGLTGAKFGCGTA
                     LCGACTVIVGKDAVRSCLTPIRDVAGKRVLTIEGLGKEGQLHPIQKAFLKHHAFQCGY
                     CTPGMILNAYALLLKKPQPTHADIVGHLDDNLCRCGSHVRIIAAIQEAASAMKGGER"
     gene            complement(14075..>15036)
                     /locus_tag="VLE22_16515"
     CDS             complement(14075..>15036)
                     /locus_tag="VLE22_16515"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HSB16061.1"
                     /translation="SRDGDTVVWMGTKNRQHILGHLGVLGRVVFPMSSAGPRESYHGD
                     PLWDTLADWADACRRQGGLAVSVHFPYPAGEQAADTVLGKIDAVELYPDYGLRFLHWY
                     RSLNCGYRLPVVGGTDKMGAGTAVGENRTYAQLGGALVNQANWARAVRNGNTFLTTGP
                     LLLFEADGRVPGQEIGLGQGGGNIEVLAQARSTMPLHRVEVVWNGRVVASRENHEGAR
                     EITLKERVRVPGPGWLAARCSSKVRPRLLAHTSPVYVQVPGQEVFSETAAAYMLTQIE
                     AAQTWVETLATRPDRERFERIRKVFTDSQAILRRRLHEHGIKG"
BASE COUNT         2946 a         4711 c         4569 g         2810 t
ORIGIN      
        1 ctctcggacg agcacgggcc ggcactggcg atgtaccggc ccgtagagga tggatgcacg
       61 gatagaatat aagatccggt caagctgcac agagccggac ttgtatgact tatttttggt
      121 cggtccccat ctggctcgga attgtgcttc aaatcctggt cattgccgcg ttgttgcggg
      181 gaccgtaccg aagtttcaag gtcctttttg tctactcggt tgttctcttt ctgatcacgg
      241 tcggcgaggc ggccgccttc tatgaccgcg agatatggac gaaaacgtcc cggcactact
      301 ggattggcga tgcgattctg caaaccctta tcttcgtgtt cgtgatctcc cttattcatc
      361 gcgccatggc aaccagtctg aagcgtgcca caatccggcg aatccttgtc tgcggagcgg
      421 ctctctttgt gacgtcctcg gtttacctca cacacgagcc gaagttcggc gcttggatga
      481 caaccctgag ccgcaacctg ggacttgccg cggtgattct caacctcgtc ctatgggcgg
      541 cgctgatccg ggaaccgtcc gccgaccgga agctcctcct ggtgagtggc ggactcggaa
      601 tccagatggc cggtaaagcg atcgggcact cactgcgtca actttcgcgc tccatgatct
      661 tcaccggcga cttgatcatc gtcatgtccc accttgcctg tctgtacatc tggtggcagg
      721 cattccgtag ttttgaccgc cctgcaccgg aacgatagaa tagtggcgga ttgttgcgga
      781 ccgtctgccc tgcgggctct ccttcgaaac catccgattt tcccggcctt tgcggcggag
      841 tcgatccagt ccagacagga gattcaaatg tcattgttga aagattcgtt gaatcgcaga
      901 gactttgtga ggagcgccgc cggcgtgtcc gcgacgcttg ggatggcagg tcaggctttg
      961 gcctcccgga caaggtctca gtccgggcga gtgcttggcg caaacgacaa gatcaatgtc
     1021 ggcgttattg gcgtcggcgg gcgcggcgcc tatctagcaa gggagttcgc cgagatcggc
     1081 gagaagacgg gcgcttgcaa agtggccgcg gtgtgcgacg tctggcagaa gcgcgtcacc
     1141 cagcagaagg aaaagcatca gtgcgacggg taccttgact atcacgaggt catcaaccgg
     1201 aaagacattg acgcggtcgt gatcgcaact cccgatcact ggcacgccag gatcgccctc
     1261 gaggcgatgg accacggcaa agacgtctac cttgagaagc ccatgtgcca caccgtcgag
     1321 gaagcccggc aactcgtgag caccgtccgc gagaccaagc gcgtactcca ggtcggttcg
     1381 cagacgacgt ccggcgatca gtggaacaag gcccgcaagg cgattgccga cggcatgatc
     1441 ggccagatga tcatgagcca gggctcgtat catcgcaact cgaaagaggg cgagtggaac
     1501 tggaagatcg atcccgaagc cggtcccgag gggaagggcg ataatttcct cgactggaag
     1561 atgtggatcg gcccggcgca gaagagatcc tgggatcccg accgttactt ccggttccgg
     1621 aaatactggg actactccgg aggcatcgct accgacctct tctatcacgt ggtcgccccg
     1681 atgaacatct gctggggcga gccgcagttc ccgtacaaag tgatggcagg cggaggcatt
     1741 tacgtgttca agaatcgtcc cgagaacccg tcgctgccgg atcgcgaagt gccggacacg
     1801 ttccacttga tcggggaata cgccaagggc cattcgatcg tgctgtcgtc ttcgatggcg
     1861 aattcacagc acatccctgg actcatccgc ggtcacgagg gatcgatcat catggtcgag
     1921 cacgggcaat tcgaaagctt gacacctttc atctcggtga tcccggaaaa acgcgtggtg
     1981 aacgacgagt acaaggcgaa gtttggcgac gagccgatta ggatcccggt tgaagagacg
     2041 gaaacgatca ctgtacacat cacgaacttc ctcgattgca tgcgtagccg ccagaagccg
     2101 actctcgatg tcgagaccgg cgcgagggcg caggtgctga tttcaatggc agtgcagtcg
     2161 taccgcgaag gccgtatcct ctacttcgac gagaggaact ggaaagtggt ggcgaagccg
     2221 cccagagcgt aagtggccag cggcgaaatg ggggtctgac tcacccggga cgctgacata
     2281 atcttagaag tcgaatgaac tacgagctgg ccgatctggt ggaagacgat cgaacggcta
     2341 tcgtctcgat tttcaaccac tacgtcgagc atagctttgc ggcctatccc gatgaaccgg
     2401 tgaccgatac tttctttgat cggatcctcg agatgagcaa gggttatccg gcggtgagca
     2461 ttaaggccga aaccggtgaa gtcgtcggtt tcggctttct tcacgcgttt caccccgcca
     2521 ctaccttccg ccgtacggca gaaatcacgt actttctgct tccggtacac acgggaaagg
     2581 gccttggcaa ccgcctgctc gcgtacttca tcgaaaaggc gcgagacatg gaaatcgaca
     2641 atctgctggc cagcgtctcc tcgctgaacg agcagagcct tcgctttcac gcccgtgccg
     2701 gattcgaacg ctgtggaacc ttccgcgcgg tgggtcggaa gcttgatcgc gagttcgacg
     2761 tggtctggtt tcagaaacgg ctctcgtgaa gaaatggggg ctgactattt tcgccgccct
     2821 gggcgatatg gctgtctgcc cccatctctt cctacgcccc gcccactact ttgatgccga
     2881 gttcccgcaa ctgatgctcc ggcaccaggg acggtgcatc gcacatcagg tcggttccac
     2941 gagcggtctt ggggaatggg atcacgtcgc ggatcgaagt ctctcccgcc aggatcatca
     3001 ccagacggtc aagcccgagc gcgatgccgc catgcggcgg agcaccgtac tcgagtgcat
     3061 cgagcaggaa tccaaacctt ctgcgagcct cttcgtcgct gaagcccaaa gcctcaaata
     3121 ccttcgactg cacgtcacgg cggtggatac gaatcgaacc agagcccaac tcgactccgt
     3181 tcaggaccag gtcgtacgac ttggcgcggc agttggccgg atccgttgtc agcttgtcga
     3241 tgtcctcgtc catcaccgat gtgaatggat ggtgggcggc gtcccagcgc ttttcgtcct
     3301 cgttccactc gaacatcggg aaatccacca cccagagaaa ctgaaaattc ttcgggtcga
     3361 gtaaaccatg ccggtcgttg tacttctgcg cggcgtagag gcgcaattgc ccgcacgcca
     3421 tgaacacggc atcaatcgga cgttgtcctt tgggcgcacc aggccacccg gcgagcagca
     3481 gcaggtcgtc ctctgcggcg ccggttcgtt cgcgaaccgc ggctatcggc tccgggaaat
     3541 cccgctccag acgcttcacg tcgtcgtata cccgaaggcc acgttccgtt ccgaacgcct
     3601 tcagctcatc gcgttccttc cgtgaaagga cgccggtctt cggaatgcgg atcgccacca
     3661 gcggcaatcc ctcggttgtg aggttcgcgc ccgggaaaag gtcttccaca gcatggaacg
     3721 gcgggagtcg gagatccggt ttgtcgatgc cataggaacg catcgcctgg cgataagtca
     3781 gccgcgtgaa cgggaaccgc acgtcgtggc cggccacggc gcagacgcgc ctgacaagcg
     3841 gctcgatcac ctcgaagatc tgttcttcct gcgggaacga catctcaagg tcgatctgcg
     3901 tgaattcggg ctgccggtca gcgcgcaaat cctcgtcgcg aaagcagcgc acgatctgga
     3961 agtacttctc gaacccgctg accatcagaa gctgcttgaa aatctgaggc gactgtggca
     4021 gcgcataaaa gtgcccccgc tggacacggc tcggcacaag gtagtcgcgg gcgccttccg
     4081 gtgtcgagcg cgtcatgaag ggcgtctcga tttcgagaaa gcccatcgaa ttcagcgcct
     4141 cgcgcaccgc gaaggagatc ttcgatcgca ggatgatgtt gcgctgcatg tggggccggc
     4201 gcaggtcgac gtagcggtac ttcagcctca cgtcctcgct cacgtcgacc gtctcctcca
     4261 tcgggaacgg cggtgtgcgc gactcgttca atatccacaa cttctccgcc accacttcca
     4321 cctcgccggt ttcaatcgcg gaattgacgg tctccggcgt gcgtttctga aggatgccct
     4381 cgacggcgat gacgtactcc gagcgcagca actccgcttt ctcgtggacc ttcgattccg
     4441 tgccggcatg gaagacggcc tgggtcacgc cgtcgcggtc tcgcaggtgg atgaagatga
     4501 cgccgccaag gtcgcggcgc cggtgcaccc agcccatcag aagaacgcgg gagccggcgt
     4561 cgctcgcacg aagttgcccg catgtgtgag ttcgtctcaa ctcgcctaga aaatccaggg
     4621 gtacgctttc cggcatagat gattcctgcg gttatagcct ttcgaacagt tcggattcgt
     4681 tgacagtctg ccgcccgccg gacgtcatgt ccttcaactg aaaggttccg gcggcgatct
     4741 cgtcgtcacc cacgatcaac gcgaagcggg cgcccgtttt gttcgccagt tccagggcgc
     4801 gcttgagtcg cgcgtcggca acgacctcga cggacttgcc ggacagcctg agccgccggg
     4861 caaggggagc gactttccgg aacgcggctt cgcctaacgg aacgacgaac aggtccaatg
     4921 cgcgggtccc cgcgcccgtg ttcgattcct ccagggtcag cacgagacgg tcctcgccga
     4981 tggagaaacc gattcccggg gagtgaactt tggacccgag cgcttcggca agtccgtcgt
     5041 aacgcccgcc gccgagcacc gaattctggg caccgagggc gccgtgcgtg atttcgaacg
     5101 tcgtgcgcat atagtagtcg aggccgcgca ccaagcgggg ccggacttca aaggcgatct
     5161 ccctgtcctg gagatagccg cgcaccgtct caaaatgctc gcggcaggcg acgcacaggt
     5221 aatcgaggat cgacggcaac gattcgatta ccggctggtc cgcttccacc ttgcagtcga
     5281 gaacccggag cggattcgtc tccgctcgac gccggcaatc ggcacacagc gaggaggaga
     5341 ctttgcgaag ttcctctcgt aaggcggcca gaaacttcgg ccgacactcg gaacaaccga
     5401 cggaattgag cagcaaccgg aagcccgcga gaccagcgcg gctgaggatc tcgaccgcga
     5461 gctcgattac ctccgcatcc acagcgggcg attccgagcc gatggcctcc gcgccgattt
     5521 ggtagaactg ccggtagcgt cccttctgcg gccgttcgcg ccggaacatt gggcccatgt
     5581 agtacagctt catcgtgccg ggctgttggt ccagccggtg ctcgatatag gcgcggatca
     5641 cggaagccgt acactccgga cggagtgtca gcgaggagcc gtccttgtcc tcaaaggtgt
     5701 acatctcctt ggtgacaatg tcggtttcct ccccgacgcc gcgcgcgaaa agcagcgttt
     5761 cctcgaaaat cggcgtgcgg atttcgtggt agttgtaagc gcggaagacg tcccgtgccc
     5821 tgtgctccac atcgttccag acgccgctcg atggcgggag gatgtcacgt gttcctttga
     5881 ccgcccgaat caaggttccg tttccttatg gttctttggc ggcgagccga ctcgccgctg
     5941 atattggtga atgcgggcaa aacgaccccg gagaggggtg tcccaacagg cacgaatcac
     6001 gatttttcgt tgaattcttc atgctagcac acggttgcgc gatactggtt ttcttatgtt
     6061 ccgcgccttc atcattttcc tcgcgctgac agcgtcgacg caggcgcaga agctgccgtt
     6121 tgacgtcgac gcccttctca agatcgcccg cattggtgat ccccagatct cgccggacgg
     6181 cacatcggtc gccttcaccg cgcaacgcgt cgatttcgac aagaatacac tggccacaca
     6241 catttacgtg gtacccgtgg ccggtggaac gcccaggcag atcacctctg cgggttctcg
     6301 caacgagcgc ccgcgctggg cgccggactc aaagacgatt gcgttcgtgt cggaccgtgg
     6361 cggaaccggc caggtgtggc tgatggaccc cgacggcgct cacgcccgcc aggtgacgaa
     6421 tctctcgacc gaggctggtg atgtcgtctt ctccccaaac ggcaaacaag tgttcttcac
     6481 gagcaacgtg taccccgaat gcgtcgacga cgcgtgcaac aagaagaggc tggaggcgga
     6541 gcgagcgtcg aaggtgaagg cccgcatcta cacatcgctg ctctaccgcc attggaatga
     6601 gtggcgcgac gcccggcgca agcacgtcct ggtggcggat ctcacgggcg gcgcggtccg
     6661 ggatctcacc ccgggcacac gcgacgtacc gccgttctcc ctcggaggac cggacgactt
     6721 cgccgtatcc ccggatggcg aggagctctg ctatgttatg aaccccgatc cggagcctgc
     6781 caccagcacg aactcggagt tgtacgtcgt cccagcgtcc ggtggggagg ccacgaaaat
     6841 caccgtcaac gtcggcgcgg acgcttctcc ccgctattca ccggacggaa agcacatcgc
     6901 ctaccgggcg caggtccgcg gggggtacga gagcgaccgc tggcggttgg tggcgatgca
     6961 gcgggacacg ggagagtcgg tcatcctcac cgagagcctc gaccgctccg tgagcagctt
     7021 cacctggtcg ccggattcga cgcggctgtt cttcaccgtc gaggatcgcg ggcggaaggt
     7081 ggtccagatg atcccggtga ccggcggtgg aatcagggtg atcgcaagcg gcgccagcca
     7141 gatcgacgat gtgcagctct ccgctgacgg aaaggtcatg atctacgccg agcagcgagc
     7201 ctcgcgtccg gccgagatct ttcgcgcttc ctcaacgggg ggcgcggcgg ttcctctcac
     7261 gcgtctcaat gacctgcacc tcggcaagta tcaattgacg gccgccgagg aattctgggt
     7321 cgacggtgca gacggagcgc acgtccacaa cttcctcgtg aaacctcccg ggttcgtgga
     7381 gggcaagaag tatccggtgt tgtttctgat ccacggcggg ccgcaaggcg cctggggaga
     7441 ggactggacg taccgctgga acgctcaggt cttcgccgga gccggctacg tggtcgtgat
     7501 gccgaacccg cgtggttcca ccggctatgg agagaagttc accgacgaga tctccggcga
     7561 ctggggcggc aaggtctacg acgacctgat ggccgtcgtc gattatgtgg caaaacagcc
     7621 gtacgccgac gccggccgga tggcagccgg cggaggatcg tacggcgggt acatggtgaa
     7681 ctggattctc gggcactcca accggttcaa ggcgctcgtt tcacacgcgg gtgtgtacga
     7741 ccttcgcagc atgttcggtg aaaccgaaga gttatggttc ccgctgtggg acttcccggg
     7801 cgcgccatgg gataacccgg aaacttactc gcgctggtca cccagctact tcgccaaggt
     7861 gtttcgaacg ccgacgctcg tcattcacgg agagctggac tatcgggtac ctgtcggcca
     7921 gggcctgcaa ctgttcaccg cattacagct ccggaaggcg ccgtccaagc ttttgctgtt
     7981 cccggacgag ggacactggg tcaacaaacc ccagaacagc gccctctggt acaagacctt
     8041 tatcgactgg atcgacacat gggccaagaa cgccggaaca tcgggcgaca agtccgaata
     8101 gcgttgctgc tgtcgctgct ggcaaccgcg gcgattgcag ccgagacgtc tcaggagcag
     8161 ttcgaccgtc tgatgaagga cgttctcatc gcggacctgc acgtggacac tccgatgtac
     8221 gttgtcgacg agggctatcg cctcggcgaa gaacatgcgt actgcgagta cgacatcccg
     8281 cggatgcgca ggggaggtct cggcgcggtg ttcttcggga tctacgtgca gccgcaggac
     8341 tacccgccgt cggtctggat gacgcgtgtc ctcgagagca tcgacgcggt tcacgaggag
     8401 gcgcggcgca acgcgaagga catcgaggtg gcctacaccg ccgcggacat cgagcggatc
     8461 aggaaggccg ggaagatcgc ggtcctgctg agcctggaag gagggcacct catccaggac
     8521 agcctggctg tgcttcgaaa cttccaccgc ctcggcatcc gctacatgac gctgacccac
     8581 ttccggacga acaactgggc ggactcgggc acggatgcgg cggtacacaa cggcttgtcc
     8641 ccactcgggc gggaagtcgt gagggaaatg aaccgcctgg ggatgatgat cgacgtttcg
     8701 cacgtctctg acaagacctt ttacgatgtt ttgtccgcat cccgcgttcc cgtgatcgct
     8761 tcccattcct gcgctcgcgc catctgcgac atcccgcgaa acatggacga cgacatgctg
     8821 cgcgccatga agaagaacgg tggcgtggtg ttcgtgaact ttagcatcgc ctacctggac
     8881 aagaaggcgt acgacgtgtt catctcgtat cgcgacgagc gagaccgtga gatcgccgaa
     8941 acgctgggag cacacgccgg cagccctctc cggtgggaac tgaagcgagc catccagcag
     9001 cggtaccgcg cgcggctccc gaaggtggat gtcaaggccg cgttgcgcca catcgaccac
     9061 atcgccaaag tggcggggcc ggatcacgta gggatcggtt cggacttcga cggcatctcc
     9121 ggaatggttc cggcagggct cgaagacgtt tcgaagttcc ccgccctgat ccgcgggatg
     9181 atcgatctcg gctactccga cgacgacatc cgcaaaatcg ccggtggcaa cctcctgcga
     9241 gtcatgcgcg ccaacgagta aacccgggac atgcagaatc aaagcgctgt caccctaaga
     9301 cttgaacccg ctgatgctgc gggcgtatca ttcccccagt aattgctctc tgggaggcca
     9361 acaccgtgcg tacattcatt tccaggattc tcgagcgcta cgaatccggc ggtttgagcc
     9421 ggcgagacct gatccaggcg ctcgcatcgc ttgccgctgc ggctccggcc gcgtcggcag
     9481 cccggtcaac cttcaaagga gtggggttga atcacatcgc cgtgcgcgtt accgacatcc
     9541 cgcgctcaaa ggcgttctac cagggcctgc tgggactgcc gctcatgagc gagtccagtg
     9601 gcagttgttt tctcaaactg ggcgacgagt tcctgacgct gtttcagaac cggaacccgg
     9661 ggctcgatca ctactgcgtg gagatcgagg cgttccgacc cgacgaggtt atggatcagc
     9721 tcaaaagaca gggactccaa ccccgccgcc ctgacggaac ggatcgaatc tacttccgag
     9781 acccggacgg gctggaggtc caactcgccg cagtcggcca tcaggcttga tccaagtcgc
     9841 ttgcagcgtg caggttagcc actcctgtcc cgtagccggc cgcggttcca gatccagtac
     9901 atcggaatac cagccgctat cagcaccgcg cccaccagcg aagagcccgg ccgggtggtg
     9961 aaggtgttca cgacgaagcc gaatgcaaag atcgtaaaga gtaacggcgt gaacggatac
    10021 ccgaagatcc ggtaggggcg gggccgctcg gggtgacgcc ggcgcaggac gatcaccgcg
    10081 aaaacggtga gcgcgtggaa gagccacatg gcgaagagga cataagaaag cagtgtttcg
    10141 taagaaccgc tgagcgccag gacgatcgcc cagacgctct gcgccgcgat ggagaaggag
    10201 ggcgtgcgga agcgcggatg gacttcacct agtcgccgaa agaagagacc gtcacgggcc
    10261 tgcgcgaagt acaggcgcgg gctggtgagg atggcgccgt tggccgctcc cgcgctggac
    10321 agcatgatgg tcaaagcgat aactatcgct ccggccggcc ccatagtttt cgctgcagct
    10381 tcggcggcca cgcgctccgt cgccgcgatg gcgcccagtg gcagcacgtg catgtaggcg
    10441 aggttagcga ggagataaag cagcatgact gcggcggtgc ccagcgccag cgagagagga
    10501 aggttacgtt gcggttcgcg cacttctccg gcgacgaacg aaacgtattg ccacccgtcg
    10561 tacgccatga agcagccgag catcgcgaca acgattccgt ccaaggacag tgagccccag
    10621 gcgaaactcc cgcccgccag tgccggcggc cggcgcagta acgcacttgc gatgagcacg
    10681 acgagcccag ccaccttcag gaatgtgaac aagttctgtg tccgggcgcc agctttaacg
    10741 ccgcggtagt tgatgaaaga gaaagccgcg atcgctgcag gtgcccagag atagcccggc
    10801 gcctgcggca gcaaatagga cgcgtagata ccgaagccga cggagaccgc ggcgatcgag
    10861 ccggactgaa cgatgagaaa cgccgaccaa ccgcaaagga aagccgtgag ctcgccgtac
    10921 gcttcgcgca gatagacgta ctgcccgccg gaatccggca tcatggcccc gagttcggcg
    10981 taggccaatg cgccaaagaa ggagatgatg ccgcaagccg tccaggtcgc cagaatcgcc
    11041 gcggacgacg ggatctctcg tgcgattgaa cccggaacaa tgaagatccc ggcaccgacc
    11101 atcgtcccaa tgacgatcga agtggcgtcc aacagcccga gtttccgcgg caatccgtcc
    11161 tggccggtgc tcatcgcatt ttgcatgcaa gcagtatgta gctattgacc gggtgccgcg
    11221 gtcggcgcta gagcctcctc ggcgaattat cctcgccgga gcgcctccag gatgcgagcc
    11281 ggagtcatcg gcaactgaaa tagtctggct ccaacggcgt catggactgc gttcgcaacg
    11341 acggcgccca ttgtgacaat tggcggttcg ccgcagcccg acgctggcac ttccgggttc
    11401 tcgatcagga ctgtctcgat tttcggcagc caggaaaagc gcgggatctg atagctgtcg
    11461 aaattgtggt cgaggacctc gccattcttg aagtgcacct cctcggtcag cgtatagccg
    11521 agacccatgg tgatgctgcc ttccatctgt tgtcgcgaac cgtccggatt gacaatgacg
    11581 ccctgatcct gcgcggaaac gacccgccgg acctcgacac gcccggtgct cctgtcgacc
    11641 gccacctccg ccatagtggc gacatacgta cccagataga tggcgcaggc gacgcccacg
    11701 ccgcgcccgc tgggcgcccg cccggacgtc cagtcgaagc gtttcgccgc ggcttccaga
    11761 acgcgctgca tgcgcttgtc cgtcaggtgg cttaaacgga actcgagagg atcgatgccg
    11821 gcctttgccg cgaggatgtc catgtgcgac tcgcgggcga aggtgttcgt attgaccgac
    11881 ggcgcgcgcc acgggccaac gctgaagggg tgcagccccg cggggttccc tccaccccag
    11941 ccaccggatg agaccgtcct ctggtgaggg atgtcataga actgctttgc ctcccggtcg
    12001 cctgcgcaat agacgctgta gtcccagaac gaaatccttc cgtcgccggt aaggcctgag
    12061 cggatcttca cgacagcagc cggccggaat gtgtcataga agaactcctc ggcgcggtcc
    12121 caaaccacct ggacaggttt gccagtaaac ttcgccaacc gggctgcttc gacggcctgc
    12181 ctggaggaac tcttgccgcc aaagccgccg ccgacatagg gggtgatgac tcgcacgttc
    12241 tgcggagtga agccgagcgc ctgcgccacc tgctgtctaa ccgggaaggg cgtttgggtg
    12301 gaggcccaca cggtggcttt accgtcctcg atcgtcgcgg tcgcggagtg cgtttccatc
    12361 gctgcgtgcg ccacatagct gttgagatac gtctcatcga cgatggccgc cgcgcgtctc
    12421 tcaccttccg aaagactgcc gctttcggcc agcacctggc cggccggcgc agtcttgagc
    12481 aggtggtcga agatcgcctg atcgtccacg ccgggcctcg ggagctcaaa ctgagccctc
    12541 atcaatccca acgcctgatc ggcaaggtcg cgccgctcgt ggagcacggc aacgaagtcg
    12601 ccttccttca ccacacgaac gccaggtact ctctcagcgg cgctcgtgtc cacatctttg
    12661 agagttgccc cgtgcgccgg cggcctgaga atccgcgcat gcagcatctc tggaatcgcc
    12721 atgtcgccgg cgtatctggc ctgtcccgtg actttctcga tggcatcttt gcggcgaggc
    12781 gactgcccga cgacggtgta tgccgccaca ggcttcaccg gcgccttctc gatgtgccgc
    12841 tcaatgcgct tcccctgaac caattgcgcg taggtaacac gcttgcccgg cgccgatggg
    12901 tccgcaacga cgccggcctt cacccggagc cggccggcct ccacctggag atgctcggcc
    12961 gccatttgga gaaggacggc ccgggcctcg gctccggctc cgcgcaggat tgggccgaac
    13021 tgccggatgc tcagcgagcc gaacgtgccc atgtcccatg gacagatgtc ggtgtcgccc
    13081 atcaccatgt ccaccgagtc gagcgcgacg tccaactcct cggcgaggat ctgcgcgagc
    13141 gccgtcttcg acccctggcc tagctcgacc ttcccgacga gacacgtgac ccagccgccg
    13201 gcgtcgattc gaaggtacgc gttgaagtct gtcgggtaac cctgcctggc cggaagccgc
    13261 gccggctcct gaaatgcggc cagtggctcg agagggaaaa acacgaatag gcccgactcc
    13321 gccactgaga ggaagtcgcg gcggctcaga tcgtccgttc tcatcgctca cctcccttca
    13381 tcgcactggc agcctcctgg atggctgcta tgatccgaac gtgcgagccg cagcggcaca
    13441 ggttgtcatc gaggtgaccg acgatgtcgg catgcgtcgg ctggggcttc ttcagcagca
    13501 gcgcgtatgc gttgagaatc attccgggcg tgcagtagcc gcactgaaac gcatggtgtt
    13561 tgagaaacgc tttctggatc gggtgaagct gcccctcctt gccaagaccc tcgatcgtca
    13621 gcacgcgttt gccggcaaca tccctgatcg gggttaagca ggacctgaca gcgtccttgc
    13681 ccacgatgac cgtacacgca ccgcaaagtg cggtgccgca gccgaacttt gcgccggtga
    13741 gtccgagatc gttgcgcaac acccacagca gcatgcgctc gtcatcgacc gtcacgcgcg
    13801 tcggcttgcc gttcagcgtg aatgaaaccg ttcgctgcat tgcaatctcc ctggcttcga
    13861 tttctgtcga atcacccgcc gtccggtctc gcatcgcaca gcttgtttag accatgtatc
    13921 cccaggattc cattccggac ccctggtgga cagcagtata cacccgcctg cctctcttgc
    13981 cgttgagcaa ttcgccgcga atgtcagctt aaggcgtgag cggctcgggg ccgccaaggc
    14041 gtatccggtt tcaactcggt ttctgacagc ccgatcagcc cttgatccca tgctcgtgca
    14101 gcctgcgacg caggatggcc tgggagtcgg taaaaacctt gcgaattctt tcgaaacgct
    14161 cccgatccgg ccgcgtcgct agggtctcca cccaggtctg cgcggcctca atctgcgtca
    14221 gcatgtacgc tgccgccgtt tcggagaaaa cctcctgtcc gggaacttgc acatacaccg
    14281 gggaggtgtg cgcaagcaat ctgggacgca ctttcgagga gcaacgcgca gccagccaac
    14341 ccggtcccgg cacccggaca cgctccttca gcgtgatctc tcgagccccc tcatggttct
    14401 ctcgcgaggc caccacacga ccattccaca cgacttccac gcgatgcaaa ggcatggtgg
    14461 accgtgcctg agccagaacc tcgatgttgc cgccgccctg ccccaagccg atctcctggc
    14521 ccggtacacg gccgtctgct tcaaagagca gcagcgggcc ggtggtcagg aatgtgttgc
    14581 cgttacgcac cgccctggcc cagttggcct gattcaccag cgcacctccc agttgtgcat
    14641 aggtgcggtt ttcgccaaca gccgtccccg ctcccatttt gtcggtcccc cccactaccg
    14701 gaagcctgta gccgcagttc agggaacggt accagtgcag aaaccggaga ccatagtcag
    14761 gatagagctc caccgcatcg atcttgccca ggacggtgtc cgccgcctgc tcgcccgccg
    14821 gataaggaaa gtgaacggac acggccagtc ccccctgcct gcgacaggcg tcagcccagt
    14881 ccgccagagt atcccacaga gggtcgccgt ggtagctctc ccgcgggccg gcggaggaca
    14941 tcggaaacac aactctgccc agcacgccca agtgaccgag tatgtgttgc ctgttcttgg
    15001 tccccatcca cacgaccgtg tcgccatcgc gcgaca
//
LOCUS       DATIFQ010000346        42423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1264993, whole genome shotgun sequence.
ACCESSION   DATIFQ010000346 DATIFQ010000000
VERSION     DATIFQ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 42423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42423
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264993"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VLE22_16520"
     CDS             <1..255
                     /locus_tag="VLE22_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16062.1"
                     /translation="VTGVQTCALPIFLLLLHKGSDIASELLFQPATKWPDRTPHDVVN
                     NMVWRVIATCCSAFALVRLEIHLPGRQGLPDFPLKVAALA"
     gene            complement(262..1560)
                     /locus_tag="VLE22_16525"
     CDS             complement(262..1560)
                     /locus_tag="VLE22_16525"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HSB16063.1"
                     /translation="MKKPYQIDSQRAVKQLEAMAADGNPAVQMVLPMAEMLGWLHKGV
                     GELIRQAGVQLMGLLMEAEVRERVGERSQRQTDRTANRWGSERGYCVVMGQKVPIERP
                     RVRTIENQEVKLGSYEMFHRGVPLTETVWEKLMLGLSTRRYGQAVREFAEAYGLEKSA
                     ISEHFIESSRAKLQEMMERRLDKMKLCALLIDATPFAGQQMVVALGISQDGRKTILGI
                     RQGATENATVVGELLGDLVNRGLDFREPRLYVLDGGKALHTAVKKYAGESAPIQRCQV
                     HKRRNVLDHLTDEQKPSVAKKLNAAYALEDHAAAKLALTQLHRELMDLNPSAARSLEE
                     GLEETLTVHRLHMPPQLRKTLASTNVIESAFSIVERVCSNVKRWHGGDQRERWVGSGL
                     LVAEKQFRRIQGYKLIPILLRELESLVSSRPEVVKRRKAS"
     gene            complement(1777..2082)
                     /locus_tag="VLE22_16530"
     CDS             complement(1777..2082)
                     /locus_tag="VLE22_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16064.1"
                     /translation="MKWREIKRLVIDRLPHQARLFWMQFGIDKTLGRKRFPRPGRWLG
                     LHDIASGAVSLADKGGTVVLWSSEAGDRSIWWGALKRGGPRKVADRLVHNLKDAMGM"
     gene            2402..2509
                     /locus_tag="VLE22_16535"
                     /pseudo
     CDS             2402..2509
                     /locus_tag="VLE22_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234468.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(2501..3067)
                     /locus_tag="VLE22_16540"
     CDS             complement(2501..3067)
                     /locus_tag="VLE22_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16065.1"
                     /translation="MFKRLRGEKPTITYIDKSTTINIADGQAHKDTVRLYTDDGVRDA
                     CRRVVAPLDREGIDLLEVRRGPVVLDAIRKEHVPCFLPVDSTDTETNLIDHTRPAVLE
                     IVRLAFKEGIKSRFWDGAVEFEAEIKDKGFMERVERGEPFRKGDSLRVRLHSRTWRTE
                     KGRLVSESTIPEVIEHVERRSPEQLSLD"
     gene            complement(3152..3403)
                     /locus_tag="VLE22_16545"
     CDS             complement(3152..3403)
                     /locus_tag="VLE22_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16066.1"
                     /translation="MSEAKFQLRYDGEAVRSGSMDVRELAPALLATGDLLQEANRVLN
                     GGRAHISVRVEAGAEKGSFPINFILDQSLLDVAKDLLLG"
     gene            complement(4109..4185)
                     /locus_tag="VLE22_16550"
     tRNA            complement(4109..4185)
                     /locus_tag="VLE22_16550"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4149..4151),aa:Val,seq:cac)
     gene            4253..5221
                     /locus_tag="VLE22_16555"
     CDS             4253..5221
                     /locus_tag="VLE22_16555"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSB16067.1"
                     /translation="MAQRDITDSPPVATSKLSELESSLGQVIRGKADVVRFSLVCLLA
                     RGHLLLEDVPGVGKTTLAQALAKSVQCTFHRLQFTSDMLPSDVLGVTIYNARTQAFEF
                     KQGPVFTHFLLADEINRTTPKTQSALLEAMNERQVTIDGRSYPLPEPFMVIATQNPVE
                     HHGTYPLPESQLDRFLLRLRMGYPDPASEREILRNSEGRLPAAARSVLDADDITQLQG
                     LAHRVTVDESLVDYMMAIVQRTREHESLSLGVSPRGAQALYRATQALAVTEGREYAIP
                     DDVKRLAVPVFAHRVVINTRVALAQRTTDVAERILQEILTQVEVPL"
     gene            5337..6410
                     /gene="ychF"
                     /locus_tag="VLE22_16560"
     CDS             5337..6410
                     /gene="ychF"
                     /locus_tag="VLE22_16560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HSB16068.1"
                     /translation="MQTAIIGLPMVGKTSLFTILTGVHEQTRVGSMETRVGVAKVPDP
                     RLEALAAVFQPEKITHATVEYLDFPSISKEALRDPACMASLRVVDALAHVLRVFESDV
                     VPHEKGSVDPLRDLHDVETELILSDLVVVEKRLERLDKDRKKIKNPELDREFELLERA
                     KAVLEANRPLREMELSAEEEKRIRGFQFLSQKPMLYVLNIGEQDTVRLHEIEAEYREG
                     PLKGIPHTAVTAMCGKIEAELTELSLDEAREYLSSYGLKEPGLERMISATYNLLGLMS
                     MLTAGETEVRAWTIPQNTPAVKAAGAIHTDFEKKFIRAEVVNWKDLVDGDGYAGAREK
                     GRLKLEGKEYVVQDGDVLVIRHG"
     gene            complement(6417..7886)
                     /gene="lnt"
                     /locus_tag="VLE22_16565"
     CDS             complement(6417..7886)
                     /gene="lnt"
                     /locus_tag="VLE22_16565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HSB16069.1"
                     /translation="MLNPLLALLTAALLISTFPGIDVALFAAVALAPLLVALAREARH
                     GRRFLLGWGAGIVYWFGVCYWIQFVLEVHGGMGTFGSWGAFLLFCLAKAVHLGVFALA
                     AGLLMRSWYAVPAVSALWVAIEATHGPLGFAWQTLGNAGIDMSVPMRLAPVTGVYGLS
                     FIFVMMNAALALVALRRSRRELFWLLPLPLLYLLPALPDAQPGAERAVLVQPNASESE
                     DWTSKSLTEAQERLAYVSLQAVLNAGKPAPKLLIWPEVPAPFYFYDDPGFRDRTMDLA
                     RLSRTWFLFGTVAHTPAGAPLNSAVLLGPDGQYAGRYDKIYLVPFGEFVPPFFDFVNR
                     ITSEAGDFEPGKELVVFQADGHRIASFICYEAVFPHLVRRFAAAGAEVLVNISNDGYF
                     GRSAARDQHLTIARMRAAENRRWILRATNNGVTTVIDPAGRISERLPSYEQAALVGSY
                     SYIGERTLYTRFGDWFAWLCATITIVVLAVSLRPLLSPR"
     gene            complement(7880..8800)
                     /gene="lipA"
                     /locus_tag="VLE22_16570"
     CDS             complement(7880..8800)
                     /gene="lipA"
                     /locus_tag="VLE22_16570"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HSB16070.1"
                     /translation="MPESLVQIAASAAARPRLPDWLRKSETRFPSVTSLKRGLRSLNL
                     HTVCESARCPNIHECFHRGTATFMILGNLCTRGCGFCSVPKGSPGKHEFNLDPDEPAN
                     VARMAARMRLRYVVLTSVNRDDLHDGGAVQWAETVREVRAALPAARIEVLTPDFCGDL
                     DAVARVLDAGPDVFNHNVETVPRLYRRVRPQADYARSLDVLQFAHQYKPGLLTKSGLM
                     AGLGETPDEVEALLRDLLGAGVDIVTIGQYLQPTRRNLPVAEYIAPRQFDAWRDYGLA
                     HGFRMVFSGPFVRSSYMADVVSTEAGAPSC"
     gene            complement(8804..9361)
                     /locus_tag="VLE22_16575"
     CDS             complement(8804..9361)
                     /locus_tag="VLE22_16575"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HSB16071.1"
                     /translation="MLKNQDGEFEVMLGNKQLLSIFFIVVILLGVFFTMGYVLGRSAG
                     PSAAMATQPEAPKAPAGSVSSFPDQPIPTRASPPAEAAPTVAQRQPEPPTVTQPKRTV
                     PPPVSELEPVPGKTYLQVAAVKRPEAEVVADVLKKKGFPSIIAPHPTEPVFRVLVGPV
                     ADADAITRMREGLEAAGFKPLVRKY"
     gene            complement(9419..10207)
                     /locus_tag="VLE22_16580"
     CDS             complement(9419..10207)
                     /locus_tag="VLE22_16580"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HSB16072.1"
                     /translation="MLLDPTIIALVIVVLYLLSAIKILAEYERGVIFRLGRVLPKPKG
                     PGIILVFAPIDRIVRVSLRLEALEVPPQDLVTRDNVTVKVNAVIYFRVIDPTKAVIEV
                     ENFRYATSQLAQTTLRSVLGEVELDDLLSQRQKLNVRLQGVLDQHTEPWGVKVTMVEV
                     KQVDLPEQMIRAIARQAEAERERRAKIIHAEGEYLAAERLAMAAELIQKQPAAIQLRY
                     LQTLVEIGQEKNTTVVFPIPIDIFSSINAMLDKAVKAAERPAQS"
     gene            complement(10226..11494)
                     /locus_tag="VLE22_16585"
     CDS             complement(10226..11494)
                     /locus_tag="VLE22_16585"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HSB16073.1"
                     /translation="MRISAIGLLAFVAGQVASGAPVVLTVDIDSVVHPVTVDIVSHAL
                     DRAKTENAAVVLIRLNTPGGLMDATRETIEKIVASPVPVVTFVTPSGGRAASAGFFLL
                     LAGDIAAMAPGTNTGAASPVVLGRELDPTMRKKVESDAAASLRSLAEKRGRNAALAEK
                     AVLEAKSFTEREALENKLIDLVAKNDAALLATLDGREITRFDGRKQALKLSGATVENY
                     QLNRRERLVKSISDPNIALVLLVLGALGVYIEFSSPGLILPGVAGGILVLLGLSALSV
                     LPINWIGVALLVLSVALFVLEAKLASHGILGAGGAVAMVLGAVLLVDSPLPEMRVHLS
                     TAIALALPFTLITVFLLTLILRARACKVVTGTAGMVGELGVAHTPLAPSGKVFVHGEF
                     WDAESTRPLEAGARVRVVSIEGLILKVEPK"
     gene            complement(11575..11650)
                     /locus_tag="VLE22_16590"
     tRNA            complement(11575..11650)
                     /locus_tag="VLE22_16590"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11614..11616),aa:Cys,seq:gca)
     gene            11948..12253
                     /locus_tag="VLE22_16595"
     CDS             11948..12253
                     /locus_tag="VLE22_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16074.1"
                     /translation="MEFLLAILAVFVLWGQVGGQEHLDYMPWYWKLVPALGLAFAAVQ
                     ATAAALEAENPWNRKTLTWLAAVAAWMVLIGAVTYYYHVTEPVEEEEEAAIESARQL"
     gene            12250..13308
                     /locus_tag="VLE22_16600"
     CDS             12250..13308
                     /locus_tag="VLE22_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16075.1"
                     /translation="MSAPNTDHETGHWRNEGYPWTIEYPRALMDRIRSAALDGFFRIP
                     RGGVEVGGVLFGNRNGKLIRIQAFRPLECEYATGPSFVLSEKDRRNLKELLAGAGSDP
                     ELAGMVALGWYHTRTRSEVFLSEADIEIYDQFFPEPWQVSLIVRPERIKPTRAGFFLR
                     ENNGSMRGESSPQEFILEPSVRQARPSAAAVEDDGRATVPEEESDLPALHRPESRQWP
                     DWLIFTAALLLFLAGAGLLSSSWWPKSAAPLSLRLIDHSGQLEVQWAQSADEARDAES
                     GSLEIIDGEKRTRLELTGGQLRLGSLTYERRSGTVYVTLRVRRAGKDASEAYAQFVGE
                     TMEEAPPWPKNLAPSVPE"
     gene            complement(13329..14510)
                     /locus_tag="VLE22_16605"
     CDS             complement(13329..14510)
                     /locus_tag="VLE22_16605"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HSB16076.1"
                     /translation="MTRRISTTLLFLLAFPVLAPSQNLYDLPPGVETRWYSFENPGGI
                     RGQGGKANEGRKGAPSRAIKAGETVVLADVTGPGTIRRIWCTLRGKTEFLRGMVVRIY
                     WDGSEQPAVQAPLQDFFGIPFARQVKFESALFSNPEARSFNSFVPMPFKKSARVVVEN
                     QAPVDVPDFFYDIEATVGDKHPEKLAYFLASYRRENPTTPKRDFEILPRIEGEGRYLG
                     SNVGIRPVSPYGEPFWFGEGELKIFVDDDREFPTMVGTGTEDLVGSAWGLGKFSHQYQ
                     GCLLSEKEDGVWGFYRYHIPDTVYFRKGIRVTLQQMAGATVEDLRKLSARDYPELVNG
                     HRRFDPSDFDGKDREWRNFEAPMDVCATAYWYQVPPLPGLPPLEPYESRIRNLGERGS
                     K"
     gene            complement(14554..15423)
                     /locus_tag="VLE22_16610"
     CDS             complement(14554..15423)
                     /locus_tag="VLE22_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSB16077.1"
                     /translation="MHEKSTRLSRRTLLRGGALSLTSGMGAADSEPAPSVTFAAVTDL
                     HYADKPPAGSRHYRDTIAKLREALAWFEETRVDFLVELGDCIDQAPDVPTETGYLKRI
                     LTELRRLRKDTHYVLGNHCVSTLTKAQFHAATGGSTGHYSFNRRGFHFVVLDACFRAD
                     GVPYGANNFDWEDSEIPPEERDWLRTDLSKTDRKTVVFVHQRLDVGPPFGIRSAAEVR
                     SILEQSGKVLAVFQGHEHTGAYGEIGGIHYCTLSAMVEGPAPDANAYSVVRLYPSGEL
                     SLEGRGNQRSYQR"
     gene            15458..17542
                     /locus_tag="VLE22_16615"
     CDS             15458..17542
                     /locus_tag="VLE22_16615"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSB16078.1"
                     /translation="MEDYNHPVRLALVALYALTVFLSAFLLFQVQPMIAKMILPWFGG
                     SSTVWSTCMLFFQTALLLGYLYAHGLRRWLGARTQAVCHGVLLAGSLAVLPVIPATWW
                     RNGWEGNPSWKILGLLAATVGLPYFLLSATSPLLQWWYARTHAGAVPYRLFALSNLAS
                     LAALLSYPVLVEPNLSTRRQAIVWSVAYACFAILCGVAGWLSARRKAEGPASGEAAGE
                     SAPKPSVWDRLFWVALAFCASTLLLAMTNQLTHDVAAVPFLWILPLSAYLLTFVLCFE
                     APRIYWRPGFYALLIPAFGVSTWMLRKGSAGLDVIPAVSVVVSALFVFCMVCHGELAR
                     AKPHPRHLTEYYVMVSLGGAAGGVFVGLVAPSLFNGYYELPIGLVLCALMAVIVLFVE
                     YRRFLETRFGMVCALALTVALGTNSGIVIVGVQATLRTYRIAKRNFYGTLRVREFDNN
                     DGYGVRRELVHGGISHGEEALFAPYRQMPGTYYCPETGAGRVLTAGQNKGARRIALVG
                     LGPGALVAYGKESDTIRIYEINPLVVQLANDEFFYLRDTPARVETVFGDARLSLEREP
                     DQNFDVLAIDAFSSDSIPVHLLTREAFAIYLRHLKPGGVLAIHISNRYVDLRPVTERA
                     ARQFGRVALLFDYHAPKDDPLCFSASWVLIVDDVARKTFSEALSVGKPIKPYPRFRLW
                     TDDFSNLYSVLD"
     gene            17559..19532
                     /locus_tag="VLE22_16620"
     CDS             17559..19532
                     /locus_tag="VLE22_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16079.1"
                     /translation="MKCVVRISARAGLPAVALMFFHGAALGGVHSPRVVSEHVPDTFS
                     LADYRNYPAWRNLQGQELALAAWKAFVSRETGVAHFQPIREGPDPIDWEFRMIRDPIK
                     MLNTYGHGFCGAFGPTTAGLFEGLGFKQGRAAQIPACNHNVTEVWYDGAWHYFDTDLR
                     GVIFRRDGKTIASIEEVREQPDLWTNPSRKVEPFFPDDPPDLQVYARTFKEQPAGYRY
                     RWFMGGSTMDYRLRKGESLTRWWRPQGGRWSHQEEDAETDFFRKLILKPPYGAKSNHP
                     NFSVWTHGNGLFEYAPVLKKGSGDFADGVFDARNVELIDNGLTLAKDGEGEAVFEVLS
                     PYVIVPLVGDLDNRGDDREASVVTFTNQGQVRVSLSLDFGRSFREIKALSDGGATTLD
                     LARHLRERYQYLVKFTLSGKRGEAVLNSLAIRTWVQVAPISLPRLMKGVNHLEYKTRD
                     QRGFASTPWMQIPNMGDQEEMSRYWVRPPADYDPARRTQRLKGEMELAFHAPPGREIQ
                     WVSLGGFFQSHQSEDAPKTANEIWYAEGDSTAWKRVHRASIPTWHSHWHYAYDTDVVL
                     DRPSATMRVRYVGNPGVNGVRVNLHSVAPVEARDSSVVITHCYRMSGREVVRKFSFDE
                     PNRYTIDCPSEPEDIYIKLEVPSDSGRSAGAGR"
     gene            complement(19512..21764)
                     /locus_tag="VLE22_16625"
     CDS             complement(19512..21764)
                     /locus_tag="VLE22_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16080.1"
                     /translation="MQRPLLILIFLLFLPTHVPGATYSKMAGELWVAGNDSFEIAVDR
                     GSGTVVRMLDKTGNVDYCRQSLRRATSDTDGNTGIDFEIGDRIAGLILFDELRDREFS
                     DLSDPGTVSALESQDGGAAILSFSKRYPGAEFVVMETFRVSDDHLRWDVRVRKTSGAD
                     RTIRVVQFAPLPLGTYDAWAPIADAPFSVKPWIPFSIDYGQSTSGAVGEGRWRTTIPM
                     MVFYSKRDKRAISFTSPFEVPAVRIRFLSSTGAGSDFHWNSRQYPARERPYFQVVNEY
                     LGARAKKDVEVSLLVSVHPADWRPALGWVYEKYRDYFDPDPGFDQWDGAFVSGYPMMR
                     DTLTQEEIRAAYADKRDRGSLWEELHGHFPWYGLMIPEPGVEKWTNASHPQDNTTLSR
                     EKIAEHCRLTKEFGIGTFLYYNVTEAEHWYATEKFPESVAKSEAGRPVGAFRADKYPD
                     KRACWLMNADPATPFGRHMIQQAKDMVTAYPEAAGFFWDVYGRSYMFDFAHEDGITMV
                     NNKPAYYPEFMFQRLMRDHVRPLLRSSGMWITANKPVTVVSTRGLDGIMTMEDAPRED
                     SPAWITAQSYLGLNRHVMILEADGGNAEMMYLHCLRYGMLDTNLRLTRRRGASLTGQE
                     IAQSRDLAKKYRPFIASLRDKKWIFHPEALELPRNTEGNIFRLKNGSVMITMVSAWRH
                     LRKGEGFNIDLEVVCRLPDAASMKTARVTAVDLDESTDVELRREGDTLRITVPKHGKA
                     TVILLSPSAG"
     gene            21802..22719
                     /locus_tag="VLE22_16630"
     CDS             21802..22719
                     /locus_tag="VLE22_16630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB16081.1"
                     /translation="MTAAIETSGITKVFRPLFRRGEIRAVEDVSLRVEPGTTFGLLGP
                     NGAGKTTFVKMLLAVVHPTSGSARILGRDVREAESRRPIGYLPENHRFPAYMTGASML
                     DFFGALSGMDRQDRRKRIPELLEAVGLSAWGGVRLRKYSKGMLQRLGLAQAMLHSPSL
                     LVLDEPTDGVDPIGRREIRDILQRLEERGVTIFINSHLLAEVELLCRNVAILHRGRLA
                     LTGKVKDLTEGGGYRVTAVNVPELLVEELRRKASACAARDGLFEFQYATREAANEAVD
                     ALRAERCDIEAIARTVSTLEEVFVRTVSA"
     gene            22712..23545
                     /locus_tag="VLE22_16635"
     CDS             22712..23545
                     /locus_tag="VLE22_16635"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSB16082.1"
                     /translation="MRNGTAGMALVGDTFREAFARLVFWGFYGLSTALILFFLFILKI
                     DIIEGARATVSLFGQSSGRAREVDELVRGLHAGIATFLYTWGMALAVFASSGLIPTML
                     EAGRIETLLSKPVPRYCILLGRYAGNLLVVAVNIAYLVLGVWLILGWKTGVWRPGFLW
                     TILTATFIFAVLLCLIVLVSVLFESAALTAMVAFGLMLMSPVLAQTKLAERLLSSEWT
                     RALWKGLYYIVPKVFDLGRMTLDLVMGKSIQTWMPVWSSALFGLAALAAGLVLFSRRD
                     Y"
     gene            23545..24471
                     /locus_tag="VLE22_16640"
     CDS             23545..24471
                     /locus_tag="VLE22_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16083.1"
                     /translation="MRFRAISCLLLLGFALAGCRSRAPETVKIDEGLAKLVPQDAVML
                     AGTRMEEVRATTAYKLWGNDADSPAIDRFARDTGFDPRKDIRELLVASDGEDTVFLAR
                     GAFAPAKLEQLLAEQGARKSTHRGQTVINNGEWSVAFLDTTTAVAGGTLAVQSSIDRR
                     LQGGGGIPAILAGRMKAIPKDSQLWIVTFGLSPALARAIPERGNMSNLRRLLSMVESA
                     SAGVDLRSGFSLNADAVGRTAEDAKFIHDAFRGLVGMGRLTVPDNEPEMLRFYDAIGI
                     TQKERTVRVQAAVPMDVLEKLAAKMRPRASSK"
     gene            24455..27244
                     /locus_tag="VLE22_16645"
     CDS             24455..27244
                     /locus_tag="VLE22_16645"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB16084.1"
                     /translation="MRVRNDKTARAGAGLILLWAAQLVAQSPATTGSIQVIVTDSTGS
                     FVAGVRVTASQEASGASRYAETDSRGELRFFGLSIGKYTLRLEKEGFASVSVAPFVVS
                     IGQTAAHRIQMDPAPVIERVDVNEAPEAVQVTATTAAVALGYDRIEEAPNRNRNYLSF
                     VLTAPGVAPSGTAGTPRPAAGARSALPDSGFVFAGMRGRNNSLLIDGVDNRDETTGGN
                     RVAIGLEMVQEFRVSGTAMSAEFGGAAGGIMNVVTRSGANIRHGDVTFFNQNDRLNAR
                     NPESLSVSRPRFRKCQPGVSTSGPIRRDRTFFSTALEQEWESGDEWSDAPEDAVEVIN
                     TALARPEFSGAAVRRVFRGLFPASGAGTEFSFKANHLASERHSLTARYAFSRGRGSGD
                     VLGAENFADRSARGSSLTRDHSLVASWIAVPGPNLVSDLRIQVARRGVELTPNVRGAM
                     LEIPGLLTLGQGYRLDADRTEDHYEVVEGLYFTRGIHQWSLGGSVHAVRASARLADRF
                     GGVFVFPTLEDFSSGRPDVFLQAFGDQRTRLLTVPAGVWLQDRWQATQGLTVEAGMRY
                     DRQWMPGTLPASNRNLAPRLGLAWRPSVGAPLVVRVGAGLFFDRYPLAFLNQALQKDG
                     RHGFEQYAAGDDAQAVMEAVRGGTSAQAIAGLARSRYDAATPFPATYARKLVAGVERG
                     FGKDTTLTVEYSYIRGFHLPRTRNAALTLPPAYSLEQSARSAYQGVSVSLNRRMSKEF
                     ALLATYSGGRTRDDGSDFDEQPSDPRNTAADWALSRQHQSHRVTASSLFELPVEDVRC
                     LPAWLRDGMSRITLAPILQAGSGRPLNALDTTDRFRTGAYPITARPFGLSRNLFLSPG
                     TFSLDLRMMKTLYYMKERGWIQFGVEAFNLTNHTNAVRVSPFYAARDVRLRSYGGVVE
                     TADARQLQLFIQVEY"
     gene            complement(27251..27622)
                     /locus_tag="VLE22_16650"
     CDS             complement(27251..27622)
                     /locus_tag="VLE22_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSB16085.1"
                     /translation="MARVTFLPAGITVEYDPETLSYSGHGKPGSVLDVALNFDFPLEH
                     ACGGSCACTTCHVIVRVGEQNLSPMDDDEADRLDMAADLTLHSRLGCQAIVTGDVTVE
                     IPDWNRNYVSESGASTLTKRS"
     gene            27678..28790
                     /locus_tag="VLE22_16655"
     CDS             27678..28790
                     /locus_tag="VLE22_16655"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB16086.1"
                     /translation="MDSDLTRRSFLTVPAGIAAAPAILRSQKGDTLRVAFIGVGNRGS
                     FLLQNMLQVQGVQVVAVCDLIPDRAAKAAAAVAAAGGKAQTYTDFRKMLDERKDIDAV
                     VQAVPDWYHKDLNIAILEVGKHLYAEKPLATNLSDCKTLVSAAKQAKGIMQVGFQLRH
                     DPNRNAAERFIHSGGIGKVLQCHGMRHGGDLPRDIPWYFDKNKCGDIVVDQGIHILDL
                     FTWAIGTHPLRVMGSGGTNLFINDPPGRTVMDNYNLIFEYPNDARVNFSHHYFDPPGF
                     TGIKERVFGAGGAVDLTEGTWQPRDKRTPPLKLDVPDAGKDSTYMALAAFVDSVKNNK
                     TPINNVDSALIATTTVLMATKAIYEKRIVTWEEMAG"
     gene            complement(28806..29948)
                     /locus_tag="VLE22_16660"
     CDS             complement(28806..29948)
                     /locus_tag="VLE22_16660"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB16087.1"
                     /translation="MNRRAFLFTPAFLPAILGAQSEEGFAPLFDGQTLNGWTIRDGPE
                     SAFYARDGEIAASPSSIHPCWLRSGRQYENFDFRCEFFVKGWIDGGVYLHAPEHGRNT
                     FCGMQVKIFHQLDKTPMSNSMGAIFPLIAPSKVNVRSDGQWNTLRMLMDWPQLSVWVN
                     GEQVQDLNVEQTPELRHRLRRGYLGLSGLGYPIRFRNLRIKELPDKEQWQYLFEADAD
                     LEKNWFVSESAERAPAKFEAMNGVLRGDGAGHLATREKYRDFVLQLYVRGPRHHNGGI
                     LIRSLGRGLSDPRHYEIQIHNVEEAHYSTGSLYHHKRSIYPRIEDDQWFLMQVVAQGR
                     KCLVRVNGEDVMEYDQLENLEEGHIELQAHQPGVWLEYKHIRVRRL"
     gene            complement(29974..30330)
                     /locus_tag="VLE22_16665"
     CDS             complement(29974..30330)
                     /locus_tag="VLE22_16665"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSB16088.1"
                     /translation="MTLEIRHEEIKPGIVLITLAGRLMLGPEGEQIENVVAQKLSEGA
                     RRFVFDLGGVTHIDSTGIGRFISSFNKVRVVDGTLRLAGATGQVRDAFRVTRLDTVFK
                     FFPDVESACEGLMPGT"
     gene            complement(30365..31258)
                     /gene="garR"
                     /locus_tag="VLE22_16670"
     CDS             complement(30365..31258)
                     /gene="garR"
                     /locus_tag="VLE22_16670"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: protein motif:HMM:NF008592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HSB16089.1"
                     /translation="MSSNIGFIGLGIMGKPMSKNLLKAGHRLVVYDIAPNPVGELVAA
                     GAAGGESSSDVAARTDIVITMLPDGPDVERAVLGRGGVLEGARKGTLLIDMSSISPLV
                     SQKVGAACAAKGVEFLDAPVSGGEPKAIDGSLAIMAGGAQEAFDRALPILQAMGSTVT
                     LTGPVGAGNVTKLANQIMVACNIAAMGEALVLATKAGLDPEVVFNAVKGGLAGSTVLN
                     AKAPMVISRNFKPGFRIRLHQKDLRNALLAAESLKVSLPFTGLVQQLLVALMNDGKGD
                     LDHSGIVTYIEKMAAVEVKKP"
     gene            31354..33066
                     /locus_tag="VLE22_16675"
     CDS             31354..33066
                     /locus_tag="VLE22_16675"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSB16090.1"
                     /translation="MMLRGLLVGLLFIAATLAAAEPPDVIYYNGKVITVWDAKPIAEA
                     LAIRGNRFASVGSNGEVLKTAGAGTKKIDLKGRAVMPGMIESHTHPIGAALSEQDGPI
                     PVMNSIAEIQAHIRKEAARLPPDRLIFVPKIYPSRLKERRYPNRYEIDEAAPGRLAMC
                     DNGYAGVLNSALLAKAGITRDTEQPENGKIIKDERGEPTGLILGAPQILSPFRRSRER
                     THADMVWAIKEMQKAYNKAGITSTADRAQGPEGLRAYQDVHGKGEMTVRTNVTYYIGA
                     RGTPKDVAAEIRSIPFVTGWGDDWLRVGPIKTTVDGGILIGTAYMREPWGMNTMIYGY
                     ADPDYRGILAVKKENLFEMARVANELGWQMTAHATGGGAIDLLLDAYEEADKVKPIRG
                     RRFNLMHSNFPNARAIERAKKLGVIFDSQIAWYHCDADALKDVFGPERMAQFLPFRSL
                     LDAGVVVVGGSDHMIRFDSRDAINPYHPFFGMWMAVTRKTAAGAVYNPEQRVTREEAL
                     KMWTINGAYNSFEENIKGSIQPGKLADMIVVTKDYLTCPEDEIRDIEPLMTMVDGKVA
                     YGGL"
     gene            complement(33067..34707)
                     /locus_tag="VLE22_16680"
     CDS             complement(33067..34707)
                     /locus_tag="VLE22_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16091.1"
                     /translation="MRGILTTLMFLGLASSKTPASDLVEIAARNAKQASEALRRSNVT
                     MHAWLKRADPVTGLLPRTGENPNWVVRDSAADLYPFMVLASRFTEPPLYHGAMRDILR
                     REVLQTTRVGRLSDDLQPGGKGFVYPTVDLDRIIFGSSEYVKDGLVPITELLGETPWY
                     HRMRGIASDIIANAPYKSRSGRLPSNSAEINGNMLQLLSRLYWKTGDETYLDQVIAIA
                     DFYFLEMLPATGYLPVHIWNLEKREAIQSLFVFSDHGNEIVGGLSEACVLVQYKRPEK
                     AKQYREPFVRMIDRVLEVGRNADGVWVTSVEVPSGKVSDARHAHCWGYMFNAIYTAWM
                     ITGDEKYRDAVRHAIAGVTRKPAYLFDETGAGRKWGSNAYSDSIEGALVLLNRLPNPQ
                     TDEALDVAVRKFFDRQRPDGIIEDWYGDGNFIRTSLMYAFRKTQGAYVMPWNTGVHVG
                     AVSDGGGVLLYLSTERAWTGRLYFDYPRHRDHWNLAVNYPRLNEWPEWFTVEHDRPYR
                     VQVGDSAPVVYLGADLVSGLRLSLPSDGQVVVQVSRGK"
     gene            34769..35542
                     /locus_tag="VLE22_16685"
     CDS             34769..35542
                     /locus_tag="VLE22_16685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB16092.1"
                     /translation="MKLKDRVAVITGAGAGIGRACALEFAKEGASVVVADIDAASARE
                     TTRQIAAAGGWALAVETDVASADSVQRLVTETRAVSGRIHVLVNNAAIQVNKTVEDTT
                     VDEWNREMAVNVGGVFLCSKFFVPHLRETKGAIVNMSSVNGFFVEPMCAGYCATKAAI
                     IGLTKAMAIDHGKDGVRVNCICPGYIDAGLAEGYFQAQPDPAAARLAAGKLHALWRIG
                     RPEEVGRLAVFLASDDASFMTGAAVVVDGGFGSGLPPTA"
     gene            35649..37412
                     /locus_tag="VLE22_16690"
     CDS             35649..37412
                     /locus_tag="VLE22_16690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB16093.1"
                     /translation="MRAAVILAGGLLAAAAVAAVVSSAVPLDVLKERLDAMAPDGTAD
                     EFPLERIAGIVWKLRLFGVLALGSAVALLAKRRQVSHVLAGVTEAAIGFAVGVTRQIF
                     RWVKSEEKVHLAALGLVFLVGVVARLWFLGQPMRDDEAATFLEFGSKPFLYGISFYPA
                     PNNHIFHTVLVHASCLLFGSEPWAIRLPSFLAGLFLIPAAYCTARILYDRHVALLTVC
                     LVSVSSPLILYSTNARGYTIVCLLFLFTLAAAFRLVGTRDKGAWTWLVMLSALGLFTT
                     PVMLFATCLGMVWLALSGWAEHRRSNRPNVLPEIVLALVAVSLLTVVLYTPALVASGA
                     AQVLGNGALAPYPWTEFPGRLRDFAHSVWSQWNMDVSPLVQWLCLAGVVISLVFHRRL
                     ARHRIPLILAVLLGCVPVLIAQRSARYARVFLFLLPVYLMLASAGISAMFQWLERRNK
                     SASYLTLAVSMIFVLSQAGYIIHAETVYHSDETGTLRNAGQIAAFLRGRLRDGDRVLA
                     DRASMYILRYYILQHHIPPGYLDPPTDRSGRLIVVSGRKSSIDARDVLDRQHIDMPEP
                     VETVMLREFSESVVYEIRAAR"
     gene            37452..39407
                     /locus_tag="VLE22_16695"
     CDS             37452..39407
                     /locus_tag="VLE22_16695"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002447.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HSB16094.1"
                     /translation="MFHQLEKRIERSVSAHLKTRYGVEVAVSVEQPKQPEFGELALPV
                     AFQLAKQLRQAPKAIAGQLAAELPAVEGVAALEVAGNGYLNVRFDRGTYGVALVRGSA
                     DAERAAADEKIIVEHTNINPNKAAHIGHLRNAVLGDTFVRMLRAGRRRVEVQNYIDNT
                     GVQVADVVVGFHFLEKKTPAEVAALIADRSQRFDYVCWELYARVSAYYHGNAEALKWR
                     QETLHAIEAGEGELAELGHLISDAIVDCHLKTMLRLGVEYDVLPRESEILHLKFWATA
                     FELLKERKGIYFETEGKNAGCWVMPAAAFSESGGDEDSKVIVRSNGTVTYVGKDIAYQ
                     LWKFGLLGKDFHYQPWMQYPDGRTLWVSTDEPRNGNVPQFGHASWVYNVIDSRQAYLQ
                     DVVAAGLRALEFNDQAERSIHFSYEMVALSPGTCVEMGIELSEEDRKRPYVEVSGRKG
                     LGVKADDLVDKLTEKALEEVDSRHPEAPVEERRKVAAQIAVCALRYFMLKYTRNAVIA
                     FDFGEALSFEGETGPYVQYAAVRARNILRKLAERGETLPDFAKDLDEAAMKRQLASED
                     FWQLVLAISKADSAVERAIASGEPAHVAKYAFHLAQAFNNFYHEYPVLHEPDRERKTF
                     LLWMTDYFRAQLERTLEVLGIEVPAYM"
     gene            39467..42136
                     /gene="acnA"
                     /locus_tag="VLE22_16700"
     CDS             39467..42136
                     /gene="acnA"
                     /locus_tag="VLE22_16700"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HSB16095.1"
                     /translation="MAVKNSFGAAASLRSGERSYRIHRLDALEKSGYGSMARLPVSIR
                     VLLENLLRHEDGVTVKSEDIEYVARWNGKPEPKEISFMPARVLLQDFTGVPAVVDLAA
                     MREALKAMGADPKLANPLLPADLVIDHSVQVDHFGTPDAFQLNASIEFQRNRERYEFL
                     RWGQSAFRNFRVVPPETGIVHQVNLEYLAPVVFSAQTNGTASAYPDTLVGTDSHTTMI
                     NGLGVVGWGVGGIEAEACMLGQPVSMLLPPVVGFKLHGKLREGVTATDLVLTVTEMLR
                     KKGVVGKFVEFYGQGLSALSLADRATVANMAPEYGATIGFFPVDEETLRYLRLTNRPA
                     EVIALVEAYVKEQGMFRTDSTPDPVFSDTLDLDLASVVSSLAGPKRPQDRIDLPNMKQ
                     NFLSGLGAEPKKTQVRLDGAEATVQDGSVVIAAITSCTNTSNPSVLIAAGLVAKKAVE
                     RGLETPPWVKTSLAPGSKVVIDYLREAGLLDYLERLKFHLVGFGCTTCIGNSGPLPEP
                     VAQAVQKEKLVVAAVLSGNRNFEGRINSLVKANYLASPPLVVAYALAGRVDLDLQNEP
                     LGTGKDGRPVYLRDIWPSNEEVRAAVGQFVNREMFGRQYSAVFDGDEQWNSIRVTGGE
                     IYKWDDASTYIKRPPYFDTPVDPATSVGNLRGMRVLAMLGDSVTTDHISPAGSIPVES
                     PAGQYLIGLGVQPKDFNSYGARRGNHEVMVRGTLANIRLRNQLAPGTEGGWTVHVPSG
                     EKMWIYDASMRYQKEGVPLMILAGKEYGSGSSRDWAAKGVALLGVKAVIAESFERIHR
                     SNLVGMGVLPLEFPAGESRESLGLTGTETFHIEGVKEAVTGGRRATVRAVAADGSEKT
                     FQAVVRVDTPREADYYRYGGILPYVLRQLAAGA"
     gene            42133..>42423
                     /locus_tag="VLE22_16705"
     CDS             42133..>42423
                     /locus_tag="VLE22_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16096.1"
                     /translation="MRRPVWTCLALTALASVAFGQAKSWRGWDTYHVIMWSTGAPRDW
                     GTWINRLREIGFTAEELTAGRDTGPFTVNNFGFYVENLVSELAYLHSRKPLYD"
BASE COUNT         8019 a        12558 c        13660 g         8186 t
ORIGIN      
        1 gtgactggag ttcagacgtg tgctcttccg atcttccttc tgctgttgca caagggtagc
       61 gatatcgctt ctgagctcct gttccaaccg gcgacaaagt ggcccgaccg tactccgcac
      121 gatgtggtca acaacatggt ctggcgtgta atagctacct gttgctcggc gttcgccctt
      181 gtccgtctcg agatacacct ccccgggcgg cagggattgc ccgactttcc gttgaaagta
      241 gcggccctcg cgtaacccac tttacgacgc cttccttcgt ttgacaacct ccggcctgga
      301 ggagactaag ctttccagtt ctctaagaag aatggggatt agcttataac cttgaatccg
      361 gcggaactgt ttctcggcca ccaggaggcc ggacccaacc cagcgctcgc gctgatcccc
      421 tccgtgccag cgcttcacgt tcgagcaaac gcgctccacg atcgagaagg ccgattcgat
      481 tacgttggtg ctggcaagtg tcttccgcag ttgcggcggc atgtgcaacc gatgcacggt
      541 cagcgtctct tccagccctt cctccagact gcgcgcggca ctgggattga ggtccatcag
      601 ctcgcggtgg agttgggtca acgccagctt ggcggctgca tggtcttcca gcgcgtaggc
      661 cgcgttcagc tttttggcca ccgacggttt ctgttcgtcc gtcagatgat ccagcacgtt
      721 gcgccgcttg tggacctggc agcgctggat cggcgccgat tcgccggcgt acttcttcac
      781 cgccgtgtgc agcgccttgc cgccatccag cacgtacaat cgcggctcgc ggaaatccag
      841 tccgcggttc actaggtcgc ccagaagttc gcccaccacg gtggcgttct cggtggctcc
      901 ctgtcgaatg ccgagaatcg ttttgcgccc gtcctgactg attcccagcg ccaccaccat
      961 ctgctggccg gcgaacggcg tcgcgtcaat cagcagggcg cagagtttca tcttgtccag
     1021 tcgccgctcc atcatctcct gcaactttgc ccgactggat tcgatgaagt gttcgctgat
     1081 ggcgctcttc tccagcccat acgcctcggc aaattcgcgc accgcctgtc cgtaccgccg
     1141 cgtgctcaac cccagcatca gtttttccca aacggtttcg gtgagcggca cgccgcggtg
     1201 gaacatctcg tagctgccca atttcacttc ttggttttcg atcgttcgca cgcgcggccg
     1261 ttcgatcggc actttctgcc ccatcaccac gcagtagccg cgctcgctgc cccaacgatt
     1321 ggccgtgcgg tctgtctgcc gctggctccg ctcgcccacc cgttcccgga cttccgcctc
     1381 catcagtagc cccatcaact gcactccggc ctggcggatc aactcgccca cgcccttgtg
     1441 cagccagccc aacatctcgg ccatcgggag caccatctgg acggctggat tcccgtcggc
     1501 ggccatggcc tccagttgct tcaccgcccg ctgcgaatca atctggtatg gtttcttcat
     1561 gtagcgactc tcctttttct ttggcgagaa atttccctga ggctaccacg cctcggggaa
     1621 acgagagtcg ctactctcta cggatttcta cgaattaccg ggcattcctc tcgcggctgt
     1681 ccatcttcag cacccggtta agcatgggtt gtcgccggat tccggacccc gacttgagat
     1741 cgctctaatc tcccgccccc gttttcggcg acgggtttac attcccattg cgtccttcag
     1801 gttatggaca agtcggtcag cgaccttgcg tggacctccg cgtttcaatg cgccccacca
     1861 gatgcttcga tctccggcct cggacgacca caggacgacc gtgccgccct tatccgccag
     1921 tgagactgct ccgctggcga tgtcgtgcaa ccctagccag cgacccggcc tcggaaatcg
     1981 ttttcgtccg agcgtcttat cgatcccgaa ttgcatccag aacagccgtg cttggtgcgg
     2041 taggcggtct atcaccaacc gctttatttc ccgccacttc atatgtcttg atctcttgga
     2101 ggcagtctgg gactagcctc ctttcttcca ctgcgcctta gcgcccttgg ctgcgatctg
     2161 tgtccgcttt ttggccggta atgccgctgc gcgtgccaga ccaccctttc gtcccaagaa
     2221 aaccgcagcc ggtctttctt tgctggcggt ttggtcttcg cggcttctcc gatggattcg
     2281 agaaagacct ggtaagcgag ctggactgga tctctgggac ggttctttgc cgtggctgat
     2341 ttcagtatgg caagcttgcc gccaaggtca agaggtcaga atttcaaact gacccactac
     2401 caacttgtgc cgagttcatc ggacgatccg tgtcacgcct gcgatggaag cgggaatctc
     2461 ggatcacgtc tggagcgtgc gcgaactgct ggaggctgat ctaatccagg cttaactgtt
     2521 ctggtgaacg ccgctcgacg tgctcgatca cctctgggat ggtagactct gacaccagcc
     2581 ttcctttttc ggttctccac gtccgggaat gcaggcgaac tctcaatgag tctcctttgc
     2641 ggaatggttc tccgcgctca actctttcca tgaagccttt atctttgatt tcagcctcaa
     2701 attcgacggc cccatcccag aacctagact tgatcccctc cttaaaggcc aaccgcacta
     2761 tttccagcac tgctggccta gtatggtcaa tcagattggt ttctgtatct gtagagtcca
     2821 cagggagaaa acaggggacg tgctctttgc ggatcgcatc aagaacgaca ggcccccggc
     2881 gaacctccag gagatcaatg ccctccctgt ctagtggcgc tacgacgcgc cgacacgcat
     2941 ccctaacccc atcgtccgtg taaagtctca cggtgtcttt gtgggcctgc ccgtcggcga
     3001 tgttgatggt ggtggacttg tcgatgtagg taatggttgg cttctctccc cttaggcgtt
     3061 tgaacatctc aaagacccca atcagtaccg cgctaggcgc tccaaggaag aagaaaagac
     3121 gccttaggat cgtttcggca tccgccagat cctagcccaa tagcaaatct ttggccacgt
     3181 ccagaaggct ctgatccaag atgaaattga tcgggaaaga acccttttct gcgcccgctt
     3241 cgactctcac ggagatgtgc gctcggccac cattcaatac cctattggcc tcctgaagaa
     3301 gatccccagt cgccagaaga gctggtgcta gttcacgtac atccattgag ccagagcgca
     3361 ccgcttctcc gtcgtagcgg agttggaact tggcttcgct cattcgcatg attctatccc
     3421 ctggtgcgag gcacacacaa ttagcgcact acccaacgtc ggaccaacga ctcgcatcgt
     3481 gcgacaatcc gtacagtcca accgttcggt cacatcggcg tttcttcttt tttgtttttc
     3541 cgctccaccg agcgccgcta tacgcggtcc cttcagtatt cccctgcact ggatcggcct
     3601 ccctcccgcc tcctagcatt tttctttttt tcacgcagac gacaggctgc gatataccgc
     3661 ccctcagagc caagtcgagg tccgcactaa attgacccac cccccccaac ctcttcgccc
     3721 tcaagcatcc tggggcagtc ccggcagagc catggcagaa ccgcctagcc tcgggattca
     3781 cccggcaaag cagaccctgc tcacgagggg cggtccgctg cacgtctgcg gacttgagta
     3841 gcctcgtcgg ctgatggttt gcctcgcaag cgtggggcga ggtttcggtg ggaggcgcaa
     3901 ggcccggacg cagcatgtca gctcgccacg aggcttctga gggccatcaa ctgctattgc
     3961 gatatgagcc ttccgcgtcc atgtggggca caacgaatac agggagggcc tcgggttgcc
     4021 gggcgagcac aacgcactcc atgtggacat ctgtgtggac gtgacgttgc agcgggtcct
     4081 ggaaggactg gtaacctatt gaaaacagtg gtgggcgcga caggactcga acctgtgacc
     4141 tcctgcgtgt gaagcaggcg ctctaaccaa ctgagctacg cgcccgttag cgtgggggcg
     4201 tgcgcggcgg aaggccgggc cttcgccgca gaccctggta ttatagcagt gaatggcgca
     4261 gcgggacatt accgactccc cgccggttgc gacttcgaaa ctcagcgagc tcgaatcaag
     4321 cctcgggcag gtcatccgcg gtaaggcaga cgtcgtacgt ttctctctcg tttgcctgct
     4381 cgcccggggg catctcctcc tcgaagacgt gcccggcgtc gggaagacca ccctcgccca
     4441 ggcgctggcg aagtcggtgc agtgtacctt tcaccggctc cagttcacca gcgacatgct
     4501 gccgagcgac gtgctcggcg tcaccatcta caacgcccgc acccaggcgt tcgagttcaa
     4561 gcagggcccg gttttcaccc acttcctgct ggccgatgag atcaatcgca ccaccccgaa
     4621 gacgcaatcc gccttgctcg aagcgatgaa cgagaggcaa gtgaccatcg acggccgctc
     4681 ctatccgctc cccgagccgt tcatggtgat cgccacccag aatccggtgg aacaccatgg
     4741 tacctatccg ttaccggaat cccaactcga ccggttcctg ctccggctcc ggatgggcta
     4801 cccggacccg gccagcgagc gcgaaatcct gaggaactcc gagggacggc tgccggccgc
     4861 agcgcgatcg gtcctcgacg ccgacgacat tacgcagttg caggggctgg cgcaccgcgt
     4921 cacggtggac gagtccctgg tcgactatat gatggcgatc gtccaaagga cacgagagca
     4981 cgaatcgctg tcgctcggag tcagcccgcg cggtgcccag gctctctacc gggccacgca
     5041 ggcgctggcg gtcaccgagg gcagagagta tgccattccc gacgatgtga agcggctcgc
     5101 cgttccggtg tttgcacacc gggtcgtgat caacacgcga gtggcgctcg cgcagcggac
     5161 cacggacgtg gccgagcgta tcctgcaaga gatcctgacg caggtggaag tgccgctgta
     5221 gaagcttctt gaaggacggg gccaagcagc tctgtccact cgcaaacaac gctcaggaca
     5281 gaggagcctg gccccgtcgg accggaagcg ggcgagcgta gtgggagttc aagttcatgc
     5341 aaactgcaat tatcggcctg ccgatggtgg gcaagacgtc gctgttcacg attctcaccg
     5401 gcgtacacga acagacacgt gtcgggtcca tggaaacgcg cgtcggcgtg gcaaaagtgc
     5461 cggacccgcg tctggaagcg ctcgcggcgg ttttccagcc ggagaagata acccacgcga
     5521 cggtcgagta ccttgatttt ccgtccattt ccaaagaggc cctgcgagat cccgcgtgca
     5581 tggccagcct gcgcgtggtg gacgcgctcg cgcacgtgct gcgcgtgttc gagagcgacg
     5641 tggtgccgca cgagaagggg tcggtcgatc cccttcgcga tctccacgac gtcgagaccg
     5701 aactgattct cagcgacctt gtcgttgtcg aaaagcgcct ggagcggctg gacaaggacc
     5761 gcaagaaaat caagaatccg gagctggacc gggagttcga gctgctcgaa cgagcaaagg
     5821 ccgtgctcga agccaaccgt ccactgcgtg agatggagct gagcgcggag gaggagaagc
     5881 gtattcgagg ctttcagttc ctgtctcaga agccgatgct gtacgtgctg aacatcggcg
     5941 aacaggatac ggtgcgcttg cacgaaatcg aagcagagta ccgagagggg ccgctgaaag
     6001 gaattccgca caccgctgtc accgcgatgt gcggcaagat cgaggcagag ctgacggagc
     6061 tgtcgctcga tgaggccagg gagtacctgt cgagctacgg cctcaaggag ccgggcctcg
     6121 aacggatgat ctcggcgacg tacaatctgc tcggtctgat gtcgatgctc actgccggcg
     6181 agactgaagt gcgcgcctgg acgatcccgc agaacacccc ggccgtgaag gccgcgggcg
     6241 ccatccatac cgacttcgag aagaagttca tccgggccga agtcgtcaac tggaaggacc
     6301 tggtggatgg cgacggctac gcaggggctc gtgaaaaggg ccggctgaag ctcgaaggga
     6361 aagagtatgt cgtccaggac ggcgatgtcc tggtgatccg ccacgggtaa gagacttcac
     6421 cgcggagaga gcagagggcg cagagagacg gcaaggacta ctatcgtgat cgttgcgcag
     6481 agccaggcaa accagtcacc aaagcgagta tagagcgtac gctcgccgat gtaggagtag
     6541 ctcccgacca gggccgcctg ctcgtaggac gggagccgct cggaaattcg gccagcgggg
     6601 tcgatgacgg tggtgacgcc gttgttggta gcgcggagaa tccagcggcg gttttcggct
     6661 gcccgcatcc gggcgattgt caagtgctgg tcgcgggcgg cactgcgacc gaaatagccg
     6721 tcattcgaga tgttgaccag aacctccgct cccgctgcgg cgaagcgccg gacgaggtgc
     6781 gggaagacgg cctcgtagca gatgaatgag gcgatccgat gtccgtctgc ctgaaagacg
     6841 accagctcct taccgggttc gaaatcgcct gcctccgacg taattctgtt tacgaagtcg
     6901 aagaacggcg ggacaaactc gccgaagggg acaaggtaga tcttgtcgta ccggccggca
     6961 tactgcccat ccgggccgag gagaacggcc gaattcagcg gagcgcccgc cggagtgtgg
     7021 gccacagtcc cgaagaggaa ccaggtccgg gagaggcgcg cgaggtccat ggtcctgtcg
     7081 cgaaaccccg gatcgtcata gaagtagaaa ggtgcgggaa cttccggcca gatcagaagc
     7141 tttggcgcgg gcttcccagc attgagaact gcctggagag acacgtaggc gaggcgttcc
     7201 tgggcctctg tgagggattt gctggtccag tcctctgact ccgaggcatt cggctggacg
     7261 agtacggccc gttcggcgcc aggttgcgcg tccggaagcg caggcaagag atacaggagc
     7321 ggcagcggga gcagccagaa cagttcccgg cggctgcggc gcaaggcgac cagcgccagg
     7381 gcggcattca tcatcacgaa gatgaaagac aggccgtaca ccccggtgac gggcgccagg
     7441 cgcatcggaa cgctcatgtc gatgccggca tttccgaggg tctgccaggc gaagccgagg
     7501 ggtccgtggg tcgcctcgat cgccacccag agcgccgaaa cggccggcac ggcgtaccag
     7561 ctccgcatga ggagacccgc cgccagcgcg aacacgccga ggtggacggc cttggccagg
     7621 cagaacagca gaaacgctcc ccaacttccg aacgtaccca tgccaccgtg gacttcgagc
     7681 acgaactgaa tccagtagca gactccgaac caataaacga tgcccgcgcc ccagccgagg
     7741 aggaagcggc ggccgtgacg cgcttcgcgt gcgagtgcaa caagcagcgg cgccagcgcg
     7801 acggctgcaa acagagcaac gtcgattccc gggaaggtcg aaatgagcag cgcggccgtg
     7861 agcagcgcga gcagagggtt cagcacgacg gcgcgccggc ctccgtgctc acgacgtccg
     7921 ccatgtagga actccgcaca aacggcccgc tgaagaccat gcggaaaccg tgcgcgagac
     7981 cgtagtcacg ccacgcatcg aattgtcgcg gcgcaatgta ttccgccacg gggagattgc
     8041 ggcgagtggg ctgaaggtac tgtccgatgg ttacgatgtc cacgccggcg ccgagcaggt
     8101 cgcggagaag agcttcgacc tcgtccggcg tttcaccgag tccggccatc aagccggact
     8161 tggtcagcag gccgggcttg tattgatggg cgaactggag gacatccagc gagcgtgcgt
     8221 agtcggcttg cgggcggacg cggcggtaga ggcggggcac cgtttccaca ttgtggttga
     8281 agacgtcagg gccggcgtcg aggacgcggg cgaccgcatc gagatcgccg cagaaatcgg
     8341 gcgtcagcac ctcgatgcga gcggcgggca atgcggcccg gacttcccga acggtttcgg
     8401 cccattggac ggcgccgccg tcgtgaagat catcgcggtt cacgctggtg aggacgacat
     8461 agcgaaggcg catccgcgcg gccatgcggg cgacgttggc cggttcgtcc ggatccagat
     8521 tgaactcgtg ttttccggga gaacctttcg gcacggagca aaatccgcaa ccgcgcgtgc
     8581 acagattgcc gagaatcatg aaggtcgccg tgccccggtg gaagcactca tggatattcg
     8641 ggcagcgcgc ggattcacaa acggtgtgca ggtttaacga tcgtaggccc cgcttgagcg
     8701 aggtgaccga cggaaagcgg gtctcggact tacggagcca gtcaggcaga cggggcctcg
     8761 ccgcggcgga cgcggcgatc tggaccaggg attccggcac gcctcaatat tttcgcacga
     8821 ggggtttgaa gcccgccgct tccagaccct cgcgcattct tgtaatcgcg tcagcatcag
     8881 ccaccggtcc gacgagcaca cggaacacag gttcggtagg atgcggggca atgatactcg
     8941 gaaaaccctt cttcttcagg acgtcggcca cgacttccgc ttccgggcgt ttgacggcag
     9001 caacttgaag gtaggtcttg ccgggaaccg gctccaattc ggagacgggc ggaggaaccg
     9061 tccgtttcgg ttgtgtcacg gtcggaggtt ccggctgccg ctgggccacg gtgggcgcag
     9121 cctcggccgg cggtgatgcg cgcgtcggaa tcggctggtc gggaaaggag gagacggagc
     9181 cggcgggcgc tttgggcgcc tctggctggg tagccatagc ggcagacggg ccagcgctcc
     9241 gcccgaggac atagcccatg gtgaagaaca ccccgagcag gatgaccacg atgaaaaaaa
     9301 tgctgagcaa ctgcttgttg ccgagcatta cttcgaactc tccgtcctgg ttctttaaca
     9361 tagagcttcc ttcgagggta ctccaacagc acccggaggt tcagttgggc cgctggcctt
     9421 atgactgtgc cggccgctcg gcggctttca cagccttgtc gagcatggcg ttgatcgacg
     9481 agaagatgtc gatcggaatc gggaagacca ccgtagtgtt cttctcctgg ccgatctcga
     9541 ccaacgtctg cagatagcgc aactggatgg cggccggctg cttctggatg agctccgcgg
     9601 ccatcgccag ccgttcggcg gcgaggtact cgccttcggc gtgaatgatc ttggcacggc
     9661 gctcgcgctc ggcctcggcc tggcgcgcga tggcgcggat catctgctcc ggcaggtcca
     9721 cctgtttcac ttcaaccatg gtcactttga cgccccacgg ctcggtgtgc tgatccagga
     9781 ctccctgaag ccggacattg agcttttgcc gctggctgag gaggtcgtcc agttccactt
     9841 cgccaagcac gctacggagg gtcgtctgag cgagctggga agtggcatag cggaagttct
     9901 cgacttcgat gaccgccttg gtcggatcga tcactcggaa gtagatcacg gcgttgacct
     9961 tgacggtgac gttgtcgcgg gtgacaagat cctgaggcgg gacctcgagc gcctccagcc
    10021 gaagcgagac tcgtacgatt cggtcgatcg gcgcaaagac gaggatgatg cccggaccct
    10081 tgggtttggg caggacgcgg ccgagcctga agatgacgcc acgctcatat tcggcgagga
    10141 tcttgatggc ggaaagcagg tacaggacga cgattaccaa cgcgatgatg gtcgggtcga
    10201 gcagcatggc aacctcccga agtttctatt tgggctccac tttgaggatc aatccttcga
    10261 tggaaacaac tcggacgcgg gcgccggcct ccagcgggcg ggtagactcg gcgtcccaga
    10321 attctccatg aacgaaaact tttccggacg gcgccaaagg cgtgtgggcg acgcccagct
    10381 ctccgaccat cccggccgtg cccgttacga ccttgcaggc acgggcgcgc aagatgagtg
    10441 taaggagaaa gactgtgatg agagtaaacg gaagagccaa cgcgatggcc gtgctcagat
    10501 gaacacgcat ctcggggagc gggctgtcga cgagcagcac ggcgccaagg accatggcga
    10561 cggcgccccc ggcgccgaga attccgtgag atgccagctt ggcttcgagg acgaagagcg
    10621 ctaccgagag gaccagaagc gcaaccccaa tccagttgat tgggagtacg gacaaggccg
    10681 atagcccgag cagaaccaga atgcctcccg ccactcccgg cagaatcagg ccgggcgagc
    10741 tgaattcgat atagacgcct agtgcgccga gcacgagcag cactaaggcg atgttcggat
    10801 cgctgatcga tttcaccagg cgctcgcggc ggttaagttg atagttttcg acggtggcgc
    10861 cggagagctt caatgcctgt ttgcggccat cgaatcgggt aatctcccgc ccgtccagcg
    10921 tggcgaggag cgccgcgtcg ttcttggcga cgagatcgat cagcttgttt tcgagggctt
    10981 cccgttcagt gaacgacttg gcctccagga cagctttttc cgccagcgcg gcgttacggc
    11041 ctcgcttttc ggcgaggctg cgaagggatg cggcggcgtc gctttccacc ttttttcgca
    11101 tggtggggtc caactcccgg ccaagaacga ccggggaggc cgcaccggta ttggtaccgg
    11161 gcgccatggc ggcgatgtcg ccggccagca ggaggaagaa gccggcggag gccgcccggc
    11221 caccgctggg tgtcacgaac gtcaccacgg gaaccggcga ggccacgatc ttctcgatgg
    11281 tctcccgggt cgcatccatc agcccgccgg gcgtgttgag ccggatcagg actacggcgg
    11341 cgttttcggt cttggcgcgg tcgagggcgt gagagacgat gtcgacggtg acggggtgaa
    11401 cgacagagtc gatatcgaca gtgaggacca cgggggcgcc ggaggcgacc tgtccggcga
    11461 caaaggcgag cagcccgatg gcggatatcc gcatcaaacc atcctcacta tatcaccgcg
    11521 ggagtgagaa ggcacggaaa accgatagta cgccaaatgt gtgtggccag aatttggagg
    11581 cgcggatcgg actcgaaccg atgatcgcgg ttttgcagac cgctgcgtta gccagcttcg
    11641 ccaccgcgcc gtgattacca tcgtagctca tgtcatgcag cctcgatcag tccccgcacg
    11701 ccccagatgt gatccgcgac gcccgccaca gacttagctc actaccgaaa accgcaaaga
    11761 agccggtgtt gcccggcggt ggcgaaggcc actagaatga gaggtctgcc agtagatccg
    11821 atgctgcttc agcggagttt acttacttct tctgcccgtg tttcggctgc cttgccggag
    11881 cgacatcccg cgggtcccgg agcgttggtc tgacatgcgt cccgcttccc tccggttagg
    11941 ccttgtcttg gaatttctgc tagcgattct cgccgtgttc gtcctgtggg ggcaggtcgg
    12001 gggccaggag catttggatt acatgccatg gtactggaag cttgttcccg cgctgggttt
    12061 ggcgtttgca gcggtacagg cgacggcggc ggccctcgaa gccgagaacc cctggaaccg
    12121 caagaccctg acgtggctgg cggccgtggc ggcgtggatg gtcttgatag gcgccgtcac
    12181 ctactactat cacgtgacgg aacccgtgga ggaggaagag gaggcagcaa tcgaaagcgc
    12241 ccgccagtta tgagcgcacc gaacacagat cacgaaaccg gccactggcg taacgaagga
    12301 tatccgtgga caatcgagta ccctcgcgcg ctgatggacc gcatccgttc ggcggcgctt
    12361 gacgggttct tccggatccc ccgcgggggc gtcgaagtcg gtggggtgct gttcggcaac
    12421 cggaatggca agttgatccg catccaggcg ttccgcccgt tagagtgcga gtatgcgacc
    12481 ggcccttcgt tcgtgttgtc cgaaaaggac cgccggaatt tgaaggaact actcgccggc
    12541 gccggcagcg acccggaact ggctggcatg gttgccctcg gctggtacca cacgcgtacg
    12601 cgaagcgagg tctttctgtc tgaggccgac atcgagatct acgatcagtt tttccccgag
    12661 ccgtggcagg tttcgttgat cgtgcgtccg gagaggatca aaccgacccg ggctggtttc
    12721 ttcctccgcg agaacaacgg aagcatgcgc ggcgaatcaa gtccacagga gttcatctta
    12781 gagccgtcgg tcaggcaggc acggccgtcg gctgcggcgg ttgaggatga cggcagggcc
    12841 acggtgccgg aggaggaatc cgatctgcca gcgttgcacc ggcccgagag ccggcagtgg
    12901 ccggactggt tgatctttac ggcggccctg ctgctgtttc tggccggagc agggttgttg
    12961 tcgtcatcat ggtggccgaa atcggcggcg ccgttgtcct tgcggctgat cgaccactct
    13021 ggccagctcg aggtacagtg ggcccagtcc gcggacgagg cccgcgatgc cgagagcggc
    13081 tcactggaga tcattgacgg ggagaagcgg acccggctcg aactgaccgg cgggcaactt
    13141 cgcctgggca gtctgacata cgagcgacgg tcaggaacgg tctacgtgac gctgcgggtg
    13201 aggcgcgcag gcaaggacgc gagcgaggcg tacgcacaat ttgtgggaga aaccatggaa
    13261 gaggctcccc cctggccgaa gaacttggcc ccgtccgttc cggagtaagt cgccgaggtt
    13321 ctttcggcct attttgagcc gcgctcgccc aagttccgga tcctgctttc gtaaggctcc
    13381 aagggaggca gcccggggag cggcggcacc tgataccagt acgcagtggc gcagacatcc
    13441 atgggcgcct cgaagttccg ccattcacgg tccttgccgt caaagtccga gggatcgaag
    13501 cgacggtgtc cattcaccag ctccggatag tcgcgagcag aaagtttccg caagtcctcc
    13561 accgtcgcgc cggccatctg ctgtaaggtg acgcggatgc ccttgcggaa atacaccgtg
    13621 tcggggatgt gatagcgata gaaaccccaa accccgtcct ctttctccga gagcagacag
    13681 ccctggtact ggtggctgaa cttccccaaa ccccacgccg atccgaccag gtcctccgtt
    13741 ccggttccga ccatagtcgg gaactcccga tcgtcatcaa caaagatctt cagttcgccc
    13801 tcaccgaacc agaacggctc gccgtaggga gaaacagggc ggatgccaac gttgcttccg
    13861 aggtatctgc cttctccttc gatccgaggg agaatttcga agtcccgttt tggagttgtg
    13921 gggttctccc gacggtagct cgcgagaaaa taggcgagct tctctgggtg cttatcgccg
    13981 acggtcgctt cgatgtcata gaagaaatcc ggaacgtcta ccggcgcttg attctcgaca
    14041 acaacgcgag cgctcttctt gaacggcatc gggacgaagg agttgaagga cctggcttct
    14101 ggattggaaa aaagtgccga ctcgaacttg acctggcggg cgaacgggat tccgaagaaa
    14161 tcctgtagcg gtgcctggac ggcaggctgt tcggagccgt cccagtagat gcgaaccacc
    14221 attccacgaa ggaactcggt tttgcctcgc agcgtgcacc atattctccg aatcgttccg
    14281 gggcccgtca catccgcaag aacgacggtt tcgcccgcct tgattgcacg agacggagct
    14341 cccttgcggc cctcgttggc tttgccgcct tggcctctga tgccgccggg attctcgaat
    14401 gagtaccacc tggtttcgac gccaggcggc aggtcataca gattctgcga aggtgccagg
    14461 accggaaaag cgagcaggaa tagcagggta gtagatattc gacgagtcat gtgcatttca
    14521 agtttaccga cttgcgaagt cgcaggcgcg cttctaccgc tgataggaac gctggttgcc
    14581 gcgcccttcc aggctcagct cgccgctcgg gtaaagcctc acgacggagt acgcgttggc
    14641 atccggcgcc ggtccttcga ccatcgccga cagcgtgcag taatggattc cgccaatctc
    14701 gccgtacgct ccggtgtgtt cgtgtccctg gaacacggcg agcactttgc cggattgttc
    14761 gaggatgctc cgaacctcgg cggcgctacg gatgccgaac ggcggtccca cgtcgagccg
    14821 ctgatggacg aacaccacgg tcttacggtc cgtcttcgac aggtcggtgc gaagccagtc
    14881 gcgttcctcg ggcggtatct cggagtcctc ccaatcgaag ttgttcgctc cgtaggggac
    14941 gccgtcggcg cgaaagcagg catcgagaac gacaaagtga aaacctctgc ggttgaacga
    15001 atagtggccc gtgctcccac cggttgccgc gtggaactgg gctttcgtaa gcgtcgacac
    15061 gcagtggttt ccgaggacgt agtgagtatc tttccggagt ctgcgaagct cggtgaggat
    15121 tcgcttcaag taaccggtct cggtgggcac atccggcgct tgatcgatgc agtcgccaag
    15181 ttcgaccagg aaatccacac gggtctcctc gaaccaggcg agcgcctcgc gcaatttcgc
    15241 gattgtgtcg cggtaatggc gtgagccggc aggcggcttg tcggcgtagt gcagatcggt
    15301 gacagcggcg aaggtgacag aaggagccgg ttccgaatcg gcagccccca tcccggatgt
    15361 caaagacagc gcgccgccgc gaagcagcgt tcgacggctg agtcgcgtgc tcttctcgtg
    15421 catatcgcag ccattgtcgc tcaatacgcc cgtggcgatg gaggactata atcaccctgt
    15481 taggctcgct ttggtcgctc tttacgcact gactgttttt ctctcggcct tcctgctctt
    15541 tcaggtgcag ccgatgatcg ccaagatgat cttgccgtgg ttcggcggat cgtcaacggt
    15601 ctggagcacc tgtatgctct ttttccagac cgcactgctg cttggctacc tctatgctca
    15661 tggactgcgg cgctggcttg gggcgcgcac gcaggctgta tgccatggtg tgctgctggc
    15721 gggcagcctg gctgttctgc cggtgatccc cgcgacgtgg tggcgaaacg ggtgggaggg
    15781 caatccgtcg tggaagatcc ttgggcttct cgccgctacg gtcggtctac cgtacttcct
    15841 gctctctgcg acgagcccgc tgctgcaatg gtggtacgcg cggacgcacg ccggcgcagt
    15901 tccgtaccgg ttgttcgcgc tttcgaatct cgcttctctg gcggcccttc tcagctaccc
    15961 ggtcctcgtg gagccgaacc tcagcacccg ccggcaggcc attgtttggt ccgtggcgta
    16021 cgcctgtttc gccatcctgt gtggagtggc aggatggctc agcgcgcggc ggaaggctga
    16081 gggacctgcg tcaggagagg cagccggcga gtccgctccg aagcccagcg tctgggatcg
    16141 gctgttctgg gtagcgctcg ccttctgcgc gtccaccctg ctgctggcga tgaccaacca
    16201 actcacgcac gacgtcgccg ccgtcccctt cctctggatt ctgccgttga gtgcatatct
    16261 gttgaccttc gtcctctgct tcgaggcgcc gcggatttat tggaggcccg gtttctatgc
    16321 gctcctcatc ccggcgttcg gcgtaagcac ctggatgttg cgcaagggct cggccggact
    16381 cgatgtgatt ccggcggtct cggtcgtcgt cagcgccctg tttgtctttt gcatggtctg
    16441 ccacggcgag ctggcgcgag ccaagccgca tccgcgtcac ctgacggagt actacgtgat
    16501 ggtatcgctc gggggagcgg cgggcggtgt gtttgtcggg ctggtggcgc cgagcctgtt
    16561 caacgggtac tacgaactgc cgatcgggct tgtcctgtgc gccctgatgg cagtgatcgt
    16621 gctgtttgtc gagtaccgcc ggtttcttga aacacgtttt ggcatggtat gtgcgcttgc
    16681 gctgacggtg gcccttggca ctaatagcgg aatagtcata gtgggcgttc aggcgacgct
    16741 gcgaacctac cggatcgcga aacggaactt ctatgggacg ttgcgcgtac gggaattcga
    16801 taataacgac ggctatggag ttcgacgcga actggtacac ggaggcatta gtcacggcga
    16861 ggaggccctg ttcgcaccgt accggcagat gcccgggaca tactactgtc ccgaaaccgg
    16921 cgccggacgg gtattgaccg cgggccagaa caagggcgcg cgccggatcg ccttagtcgg
    16981 tcttgggccg ggtgcgctgg ttgcttacgg caaggagagc gacacgatcc ggatttacga
    17041 gataaacccg ctcgtcgtgc agcttgcgaa cgacgaattc ttctatcttc gagatacgcc
    17101 cgcgcgggtg gagacggtgt tcggcgacgc ccggctttca ctcgagcgcg agccggacca
    17161 gaacttcgac gtgctggcga ttgatgcttt ctccagtgac tcgattcctg tgcatctgct
    17221 gacgcgggaa gccttcgcca tctacttgag gcatttgaag ccgggcggcg ttctcgcgat
    17281 ccacatctcg aaccggtacg tcgatcttcg gccggtcacc gagcgggccg cacgtcaatt
    17341 cggccgggtc gcacttctct tcgattacca cgccccgaag gacgacccac tctgcttcag
    17401 cgcctcctgg gtgttgatcg tggacgacgt cgcgcggaaa acgttcagcg aggccctgag
    17461 cgtggggaaa ccaataaagc catatccccg cttccggctc tggaccgacg atttctcgaa
    17521 cctgtacagt gtgctggatt gaaccacgga ggtgatagtt gaagtgcgtc gttcgaatct
    17581 cggcgagggc cggtctgccg gcggtcgccc tcatgttctt tcacggagcg gccctcgggg
    17641 gagtacactc cccacgagtc gtctccgaac atgtgcctga cacgttcagc ctggccgact
    17701 accggaacta cccggcgtgg cgaaacctcc agggccagga actggcgctc gccgcctgga
    17761 aggcgttcgt gagcagggag accggcgtgg ctcacttcca gccgatccgt gagggcccgg
    17821 atccgatcga ttgggagttt cgcatgatcc gtgacccgat caagatgctg aacacgtacg
    17881 ggcacggttt ctgcggcgcc ttcggcccga cgacggccgg actgttcgaa ggcctcggat
    17941 tcaagcaagg gagagccgca cagatccccg cttgtaacca caacgtcacc gaggtctggt
    18001 atgacggcgc ctggcactac ttcgacacgg acctgcgcgg cgtgatcttc cggcgcgacg
    18061 gcaagacgat tgcgtccatc gaagaagtgc gggagcaacc ggacctctgg acgaacccgt
    18121 cgcggaaagt tgagccgttc tttccggatg atccgcccga ccttcaggtg tatgcacgca
    18181 cctttaagga gcagccggcg ggttaccgtt accggtggtt tatggggggc tccaccatgg
    18241 attatcgcct acggaaagga gagagcctca cccgctggtg gaggccgcag ggagggcgat
    18301 ggtcacacca ggaggaggac gccgagacgg acttcttccg caaactcatc ctgaagccgc
    18361 catacggagc gaaaagcaat cacccgaatt tcagcgtttg gacacatgga aacgggctgt
    18421 tcgagtatgc ccccgtgctg aagaaaggat ccggcgattt cgccgacggc gtctttgacg
    18481 cacgaaacgt ggagctcatc gacaacgggc tgacgctggc gaaagacggc gaaggcgagg
    18541 cggttttcga ggtgctcagc ccgtacgtta tcgtcccgct ggttggcgac ctcgataacc
    18601 ggggcgacga ccgtgaggcg tcggttgtaa cgtttacgaa ccaggggcag gttcgggttt
    18661 ccctatcgct cgatttcggc cggtcgtttc gcgaaattaa ggcgctgagc gacggcggcg
    18721 ccacgaccct ggatctcgcc cggcaccttc gtgaacgtta tcagtatctg gtgaagttca
    18781 cgctgagcgg aaaacgcgga gaggcggtct tgaactcgct cgccatccgg acctgggtgc
    18841 aggtggcgcc catctcgttg ccgcgcctca tgaagggcgt caaccacctc gaatataaaa
    18901 cccgcgacca gcgtggcttt gcctccacgc catggatgca gattccgaac atgggcgacc
    18961 aggaggagat gagccggtac tgggtcaggc ctccggcgga ttacgatccg gcccgaagga
    19021 cgcagcggct gaagggcgaa atggagcttg catttcacgc tccgcccggt cgcgagatcc
    19081 aatgggtcag cctcggcggc ttttttcagt cccaccagag cgaagacgcg ccgaagacgg
    19141 cgaatgagat ctggtacgcg gaaggcgact ccacggcctg gaaacgggtg catcgtgctt
    19201 cgattcccac gtggcactcg cactggcact acgcctacga cacggatgtc gtgctcgatc
    19261 ggccctccgc aacgatgcgc gtacgctatg tcggcaatcc cggcgtgaac ggcgtgcgag
    19321 tcaatctgca ctccgtcgct cctgtggaag cgagagacag ttccgtcgtc ataactcatt
    19381 gctaccggat gagcggccgc gaagtagtcc gaaagttcag tttcgacgag ccgaaccggt
    19441 acacgatcga ctgtccgtcg gagccggaag acatctacat caagcttgaa gtgccgagcg
    19501 acagcggccg ctcagccggc gctgggcgat agcagaatca ccgtcgcttt gccgtgtttc
    19561 ggcacggtga tgcgcagtgt atctccctcg cggcgtagct ctacgtctgt ggactcgtcg
    19621 agatccacag ccgttacacg cgcagtcttc atcgaggccg cgtccgggag gcggcataca
    19681 acctcaagat cgatgttgaa accctcaccc ttccgcaaat gcctccaggc cgacaccatg
    19741 gtgatcatca cactgccgtt cttgaggcgg aagatgttgc cttcggtgtt gcgcggcagt
    19801 tcgagcgctt cgggatggaa gatccatttc ttgtcacgga gacttgcgat gaacggccgg
    19861 tacttcttcg cgaggtcgcg actttgtgcg atctcttggc cggttagcga cgcgccccgg
    19921 cgccgcgtca gtctcaagtt cgtgtcgagc attccgtagc gcaggcagtg gagatacatc
    19981 atctcggcat ttcctccgtc ggcttccaag atcatgacgt gccggttcag gccgaggtag
    20041 ctctgggcgg taatccaggc cggactgtct tctcgcggcg cgtcctccat cgtcatgatg
    20101 ccgtccagtc cgcgggtact cacaacagtc accggcttat tcgccgtgat ccacatgccg
    20161 ctgcttcgca gcagcggccg gacgtggtcc cgcatgagcc gctggaacat gaactcgggg
    20221 tagtaggcgg gcttgttgtt gaccatggtg attccgtctt cgtgggcgaa atcgaacata
    20281 tagctccgcc cgtagacatc ccagaagaag cctgccgcct cagggtaagc cgtcaccatg
    20341 tcttttgcct gctgaatcat gtgcctgccg aacggagtcg ccgggtcggc attcatcagc
    20401 cagcaggcgc gcttgtccgg atacttgtcg gcgcggaacg cccccaccgg ccttcccgcc
    20461 tccgatttcg ccacgctctc ggggaatttc tcggtcgcgt accagtgctc cgcttcggtc
    20521 acgttgtagt acaggaaagt gccgatgccg aactcctttg tgagccggca atgttcggcg
    20581 atcttctccc ggctcagcgt ggtgttgtcc tgcggatggg atgcgttggt ccatttctcg
    20641 acgcctggct cgggaatcat gagcccgtac catgggaaat ggccgtggag ctcctcccac
    20701 agcgaaccgc ggtccctttt gtcggcgtac gcagctcgaa tctcttcctg ggtcaacgtg
    20761 tcccgcatca tcgggtagcc ggagacgaag gcgccgtccc attgatcgaa gcccggatcc
    20821 ggatcgaagt aatcgcgata tttctcgtag acccagccga gcgcgggccg ccagtccgcc
    20881 gggtggacgg agacgaggag actgacttcg acatccttct tcgctcgtgc gccgaggtat
    20941 tcattgacga cctggaagta cgggcgttca cgcgccgggt actggcgtga attccagtgg
    21001 aagtcggagc cggcgccggt gctcgagagg aaacgtatgc ggaccgcggg aacctcgaac
    21061 ggcgaagtga acgaaatcgc gcgcttgtcg cgtttcgagt agaacaccat catcgggatt
    21121 gtggttcgcc agcggccctc gccgaccgct ccggaagtgg actgcccgta gtcgatggag
    21181 aagggaatcc atggcttgac agaaaacggc gcgtcggcaa tcggcgccca ggcatcgtaa
    21241 gttccgaggg gcagcggcgc gaactggacc acgcggatcg tccggtccgc gcccgaggtc
    21301 ttgcgcacgc gcacgtccca gcggagatga tcgtccgata cccggaacgt ttccatcacc
    21361 acgaactcgg cgcccgggta gcgtttgctg aacgagagga tagccgcgcc gccgtcctgc
    21421 gactccagcg cggatacagt gccggggtcg ctgaggtcgg agaactcgcg gtcccgcagc
    21481 tcgtcgaaga ggatcagccc cgcgatgcgg tcaccgatct cgaagtcgat gccggtgttg
    21541 ccgtcggtgt cggaggtggc ccgccgcagc gactggcggc agtaatcgac gttgccggtc
    21601 ttgtcgagca tacggacaac cgtgccggag ccccggtcca cggcaatctc aaacgaatca
    21661 ttccccgcga cccacaattc gccggccatc ttcgagtacg ttgcgcctgg aacgtgcgtt
    21721 ggaaggaaga ggagaaagat aaggatcaat agcggtcgtt gcatgggggt attgtatcgg
    21781 ccggaatgac ataatggggg cgtgaccgcg gccattgaga ccagcggaat tacaaaggtt
    21841 tttcgaccgt tattccgccg gggggaaatc cgcgcggtcg aagacgtctc tctgcgggtc
    21901 gagcccggca cgacgttcgg acttctgggg ccgaacggag cagggaagac gactttcgtg
    21961 aagatgctgc tcgcggtcgt ccacccgacc tcgggaagtg ctcgcatcct cggccgcgac
    22021 gtccgcgaag ccgagagccg ccgcccgatc ggctacctgc ccgagaatca tcgttttccc
    22081 gcctacatga caggcgccag catgctggat ttctttgggg cgttgtccgg catggaccgg
    22141 caggaccgca gaaaacgaat acccgagttg ttggaggcgg tgggactgag cgcgtggggc
    22201 ggtgtgcgat tgcgaaagta ctcgaagggc atgcttcagc ggctcggcct ggcgcaggcg
    22261 atgcttcatt cgccttcact gctggtattg gacgagccta cggacggagt ggatccgatc
    22321 gggcgcaggg aaatccggga catcctgcaa cggctggaag aacggggcgt aacgatcttc
    22381 atcaattcgc accttctagc tgaagttgag cttctctgtc gcaacgtggc gattctccat
    22441 cgcggccggc tcgcgttaac tggaaaagtg aaggacctaa cggaaggtgg gggctatcgt
    22501 gtgaccgccg tgaacgttcc ggaactgctc gtcgaggagc tgcgccgaaa ggcttcggca
    22561 tgcgctgcaa gggacggcct gttcgagttc caatatgcga cgcgggaagc ggccaacgag
    22621 gcagtggatg cgttgcgagc ggagcggtgc gacattgagg caatcgcgcg aaccgtaagc
    22681 acgctcgaag aagtgtttgt gaggaccgtg agtgcgtaac ggaaccgctg gaatggcgct
    22741 ggtcggggac acgttccgcg aggcgttcgc gcgcctcgtg ttttggggat tctacggtct
    22801 ctcgacggcg ctgattctgt ttttcctgtt cattctgaaa atcgacatca tcgagggcgc
    22861 ccgggcaacc gtctcgctgt tcggtcaatc gagcggcagg gcgcgggaag tggacgagct
    22921 cgtgcgcgga ttgcacgccg gcattgcgac gttcctatac acgtggggca tggcgcttgc
    22981 ggtctttgcc tcatcgggac tgattccgac gatgttggaa gcgggacgga tcgagacgct
    23041 gctgtcgaag ccggtgccgc gttactgcat tctgttgggg cgctacgccg gcaacctgct
    23101 ggtggtggcc gtcaatatcg cctatctcgt actgggggtg tggctgatcc tcggctggaa
    23161 aactggggtc tggcgtcccg gttttctgtg gacgatcctg accgcgacgt tcatcttcgc
    23221 cgtgttgctc tgtctcatcg tgctggtgag cgtgctgttc gaaagcgccg cacttacggc
    23281 gatggtcgcc ttcggcctga tgttgatgag cccggttctc gcacagacga agctggccga
    23341 gcggctgctc tcgtcggaat ggaccagggc gctttggaag ggcctttact acatagtgcc
    23401 aaaggtgttc gacctcggga ggatgacgct cgacctcgtg atgggcaagt ctatccagac
    23461 gtggatgccg gtctggagtt cggcgttgtt cggcctggcg gccctggcgg ccggtctggt
    23521 gctgttctca cggagggatt attgatgcgg tttcgggcca tttcgtgtct tcttttgctc
    23581 ggatttgcgc tcgcgggttg ccggtcccgg gcacccgaga ccgtgaagat cgacgaaggg
    23641 ctggcgaagc tggtcccgca ggatgccgtg atgctggcag gaacgcggat ggaggaggtg
    23701 cgcgcgacca ctgcgtacaa actctggggc aacgatgcag attctcccgc aatcgaccgg
    23761 ttcgctcgcg ataccggttt cgatcccagg aaggacatcc gcgagctgct cgtcgcatcg
    23821 gatggggagg acaccgtctt cctcgcacgc ggcgccttcg ctcctgcaaa gctcgaacaa
    23881 ctgctggcag agcagggcgc ccgcaagtcc actcaccggg ggcaaacggt gatcaacaac
    23941 ggcgagtggt cggtcgcatt cctcgacaca acaaccgccg ttgcaggtgg caccctggcg
    24001 gtccagagca gcattgatcg gcggctgcag ggcggcggag gcattccggc gatccttgcg
    24061 gggcggatga aggcgatccc gaaggacagc cagttgtgga ttgtcacctt cgggttgagc
    24121 ccggcgcttg cgagggcgat tccggagcgg ggaaatatgt ccaacctccg ccgcctcctc
    24181 tcgatggtcg agagcgcgtc ggccggagtc gatctcagat cgggctttag tctgaatgcg
    24241 gacgcggtcg gccggacggc ggaggacgcg aagttcatcc acgatgcctt tcgggggctg
    24301 gtcgggatgg gcaggctgac ggttccggac aacgagccgg agatgctcag gttctacgat
    24361 gcaatcggga taacccagaa ggagcggacg gtgcgggtcc aggctgccgt cccgatggat
    24421 gtgctcgaga aactcgctgc gaaaatgagg ccgcgtgcga gttcgaaatg acaagacggc
    24481 ccgggcaggt gcaggcctga ttctgctgtg ggcggcacaa ctcgtggcgc agtctccggc
    24541 caccacgggt tccattcagg tcatcgtcac cgatagcacg ggctcgttcg tcgccggtgt
    24601 acgggtcaca gccagtcagg aggcgtcggg ggcatcgcgg tatgcagaaa cggactcccg
    24661 aggcgaatta cgcttcttcg gactgtcgat tggcaagtat acgttgcgtc tcgagaaaga
    24721 gggcttcgcc tcagtttcgg tggccccgtt cgtggtttcg atcgggcaga cggcggctca
    24781 cagaattcag atggaccccg ccccggtgat cgagcgggtc gatgtgaatg aagcgcccga
    24841 ggcggttcag gtgacagcaa ccaccgctgc cgtcgcgctt ggctacgacc ggatcgaaga
    24901 ggcgccgaac cggaaccgga attacctaag tttcgttctg accgcgcccg gcgtcgcgcc
    24961 gtcgggaaca gcgggcacgc cgcggcccgc cgccggagcg cgcagcgccc tgcccgacag
    25021 cggttttgtc ttcgcaggca tgcggggacg gaataacagc ctgctgattg acggcgtcga
    25081 caaccgtgac gaaactaccg gcggaaacag ggtggcgatc ggtctcgaaa tggtgcagga
    25141 gttccgcgtc tccggaaccg cgatgtcggc cgagtttggc ggcgctgcgg gcggcatcat
    25201 gaacgtagtc acgcgatcgg gagcgaacat ccggcacggc gacgtgactt tcttcaacca
    25261 gaacgatcgg ctcaatgcga ggaacccgga gtctctcagc gtcagccgcc cacgtttccg
    25321 gaagtgtcaa cctggagtct cgacgagcgg cccgatccgg cgcgaccgta ctttcttttc
    25381 gactgcactt gagcaggagt gggagtcggg cgatgagtgg tccgacgctc cggaggatgc
    25441 agtcgaagtg atcaatacgg cgttggcccg acccgaattc tctggcgctg cggtccgacg
    25501 ggtctttcga gggctgtttc cagcgagcgg ggcgggtacc gagttctcgt tcaaggcgaa
    25561 ccatctggcg agcgagcggc acagcttgac ggcgcgctat gcgttctcac gaggcagggg
    25621 ttccggtgac gtgctcggcg cggagaattt cgccgaccgc tcggcgcggg gcagcagcct
    25681 gacgcgggac cactccctag tggcgagctg gatcgccgta ccgggaccga atctggtcag
    25741 tgacctgcga atccaggtag cgcggcgcgg cgtcgaactg acgccgaacg ttcgaggggc
    25801 gatgctggag atccccggtt tgctgacgct gggccagggc tatcgtctcg atgcagaccg
    25861 gaccgaggat cactacgagg tcgtagaggg cttgtacttc acgcgcggga ttcaccaatg
    25921 gagtctcggg ggaagcgtcc atgccgtccg ggcgagcgcc cgtctcgcgg acaggttcgg
    25981 tggcgtcttc gtatttccaa ctctggagga ctttagctcc ggacggccgg acgtgtttct
    26041 gcaggcattt ggagaccaac ggacacgcct cctcacggta cctgccggag tttggcttca
    26101 ggatcggtgg caagcgacgc agggtttgac cgtcgaggcg ggcatgcgat acgaccggca
    26161 atggatgccg ggaacactgc ctgcgtcgaa ccggaatctc gctccccgcc tgggtttggc
    26221 gtggcgaccc tcggtggggg cgccgctggt ggtgcgagtg ggtgccgggc tgttttttga
    26281 ccgttacccg ctggcgttcc tgaaccaggc tttacagaaa gacggccggc acggcttcga
    26341 gcagtatgcg gcgggcgacg acgcgcaggc tgtgatggag gctgtgcgtg gaggtacatc
    26401 cgcgcaggcc atcgcaggtc tggcgcgctc gcgctacgat gctgccacgc cgtttccagc
    26461 cacgtacgcg cgaaagcttg tggcgggcgt ggaacggggc ttcggtaaag acacgacgct
    26521 gaccgtcgaa tacagttaca tccgcggatt tcacctgccc agaacccgaa acgcggcttt
    26581 gacgctgcct ccggcttatt ctttggagca gagcgcaagg tcggcttatc aaggggtgtc
    26641 cgtctcactg aaccggcgca tgagcaagga gtttgcgctg cttgcgacgt attcaggcgg
    26701 ccgcacccgc gacgatggat cggatttcga tgagcagccg tccgacccgc gcaacaccgc
    26761 ggcagactgg gcgctctcgc gccaacacca gtcgcaccgt gtaacggcga gctcgctctt
    26821 cgaactgccg gtggaggacg tccgatgttt gccggcgtgg ttgcgcgacg gtatgagccg
    26881 gattactctc gcgcccatcc tgcaagcggg ttcgggtcgc ccgctaaacg ccctggacac
    26941 caccgacaga ttccgaacgg gcgcttaccc gatcacggcc cggccgttcg ggttgtcccg
    27001 caacctgttt ctttcaccgg ggaccttctc gttagacctc cggatgatga agactctgta
    27061 ctacatgaag gagcgcggct ggatccagtt cggcgttgaa gcattcaatc ttacgaatca
    27121 caccaacgca gtccgcgtca gcccgttcta cgcggcacga gatgtccggt tacgctccta
    27181 cggaggcgtt gtggagacgg ccgacgcccg ccaacttcag cttttcatcc aggtggaata
    27241 ctgagagcag tcagcttctt ttcgtcagcg tcgaggcgcc ggactcagac acgtagttgc
    27301 ggttccagtc cgggatttcg acggtgacgt cgccggtgac gatagcctgg cagccgaggc
    27361 gcgagtgaag ggtcaggtcg gcggccatgt caagccggtc agcttcgtcg tcatccatcg
    27421 gcgataggtt ctgctcaccc acgcggacga tcacgtgaca ggttgtgcag gcgcaactcc
    27481 cgccgcaggc gtgctcgagc gggaagtcga agttcagtgc aacgtcgagc actgagcctg
    27541 gcttgccgtg cccgctgtac gacagagttt cgggatcgta ttctaccgtg attccggcag
    27601 ggagaaaggt gactcttgcc acgcctcgat tattgtagcg cgggacgttg tggttagaat
    27661 cgatttagga gagatgcatg gatagtgatc tgactcgtcg ttcgtttctc acggttccgg
    27721 ccggaatcgc cgcggcgccc gccattcttc ggtcgcagaa aggcgatacg ctccgggtgg
    27781 catttatcgg agtcggtaat cgcggttcat ttcttttgca gaacatgctt caggttcagg
    27841 gagtccaggt ggtcgccgtg tgcgacctca tcccggatcg cgccgcgaag gcggcagctg
    27901 cggtggcggc ggccggcggt aaggcgcaga cctataccga cttccgcaag atgctggacg
    27961 agaggaagga tatcgatgca gtcgtacagg cagttccgga ctggtaccac aaggacctga
    28021 acatcgccat cctcgaggtt ggcaaacacc tctatgcgga gaagccgctc gcgacgaatc
    28081 taagcgactg taagacgctc gtaagcgccg cgaaacaggc gaagggcatc atgcaggtcg
    28141 gcttccagct tcggcacgat ccgaaccgaa acgcggcgga gaggtttatc cacagcggcg
    28201 gcatcgggaa agtgctgcag tgccacggaa tgagacacgg aggcgatctg ccgcgcgata
    28261 tcccgtggta cttcgacaag aacaagtgcg gcgacatcgt ggtcgaccag gggattcaca
    28321 tcctcgatct gttcacctgg gccatcggaa cccacccgct gcgtgtcatg ggttccggcg
    28381 gaacgaacct gttcatcaac gaccccccgg gtcggacggt gatggacaac tacaacctga
    28441 ttttcgaata cccgaacgat gcgcgggtga acttcagcca tcattatttc gacccgccag
    28501 gcttcaccgg gatcaaggaa cgcgtcttcg gtgccggcgg cgcagtggac cttaccgagg
    28561 gaacctggca gccgcgcgac aagaggactc ctcccctcaa gcttgacgtc cccgacgccg
    28621 gcaaagactc cacctacatg gcgctggcgg cattcgtgga cagcgtgaag aacaacaaga
    28681 caccgatcaa caacgtggac tcggcgctca ttgccaccac gacggtgctc atggcaacaa
    28741 aggcgatcta cgagaagcgg atcgtcactt gggaagaaat ggcggggtag gcgccctcac
    28801 cctttctaca gccttcgcac acggatgtgc ttgtactcga gccagacgcc gggctggtgt
    28861 gcctggagtt cgatgtgacc ttcttccaga ttctctagct ggtcgtattc catcacgtct
    28921 tcgccgttga ccctcaccag gcacttgcgt ccctgagcga ccacctgcat caggaaccac
    28981 tggtcgtctt cgatccgggg atagatggac cgcttgtgat ggtaaagcga accggtcgag
    29041 tagtgggcct cctccacgtt gtgaatctgg atctcgtagt ggcgcgggtc agagagtccg
    29101 cgtccgagcg aacggataag gatgccgccg ttgtggtgcc gcggcccgcg aacataaagc
    29161 tgcagcacga agtcacggta cttctcgcgg gtggcgaggt gtcccgcacc gtcgccgcgg
    29221 agcactccgt tcatggcttc gaatttcgcg ggcgcacgct cggcgctctc ggacacaaac
    29281 cagttcttct ccaggtcggc gtcggcttcg aaaaggtact gccactgttc cttgtccggc
    29341 agttctttga tgcgaaggtt tcgaaatcgg atcggatagc ccaggccgct cagaccgaga
    29401 taaccgcggc gcagacggtg tcgcaattcc ggcgtctgct cgacgttcag atcctgaacc
    29461 tgctcgccgt tgacccagac gctgagttga ggccagtcca ttagcatacg cagcgtgttc
    29521 cactggccat cgctccggac gttcaccttc gacggcgcga tcagcgggaa gatcgccccc
    29581 attgagttgg acatgggcgt cttgtccaac tggtggaaga tcttcacctg catgccgcag
    29641 aaggtattgc ggccgtgttc cggcgcatgc aggtagaccc ctccgtcgat ccagcccttc
    29701 acgaagaact cgcatcgaaa gtcgaaattc tcatattgcc tgccggaacg cagccagcat
    29761 gggtgaatcg atgaaggcga ggccgcgatc tcgccgtctc tagcgtaaaa cgcggactcg
    29821 ggaccgtccc gaatggtcca gccgtttagt gtttggccgt cgaagagtgg cgcgaagccc
    29881 tcctcggatt gtgctccaag gatggcgggc aggaatgcgg gtgtgaaaag aaaagcgcgg
    29941 cggttcataa gagtaggtct ccagacgtgc cgatcaggtt ccaggcatta agccttcgca
    30001 ggccgattcc acgtcgggaa agaacttgaa aacggtatcg aggcgggtga cgcgaaaggc
    30061 gtctcgaacc tggccggtgg ccccggcgag gcgaagcgtt ccgtcgacga cgcgcacttt
    30121 attgaaactc gagatgaacc ggccgattcc cgtgctatcg atgtgggtca cgccgcccaa
    30181 gtcgaacacg aaccttcgcg ccccttcgga tagcttctga gccacgacgt tttcaatctg
    30241 ctcaccctcg ggaccgagca tcagccggcc cgccaaagta atgagcacga tgcccggctt
    30301 gatctcctcg tgtcgaatct cgagtgtcat gaacccttgg tcttaagcat acagcaacgt
    30361 ggtgtcaggg tttcttcact tccacggcgg ccatcttctc gatgtacgtc acgatgccgg
    30421 agtggtcgag gtcgcctttt ccgtcgttca tgagagcgac gagcaactgc tgcacgaggc
    30481 cggtaaacgg cagggaaacc ttcagcgact ccgccgccag cagcgcgttc cgcagatcct
    30541 tctggtgcag gcggatgcgg aagccgggct tgaaattccg ggaaatcacc atcggagcct
    30601 tggcgttgag cacggtgctg cccgcaagac cgcccttgac ggcattgaac accacctcgg
    30661 ggtccaggcc ggccttcgtg gccagcacga gcgcttctcc catggcggcg atgttgcacg
    30721 cgaccatgat ctggttggcg agcttggtca cgtttcccgc gccgacgggg ccggtgagcg
    30781 tcacggtcga acccatggcc tggaggatcg ggagggctct gtcgaaagcc tcctgcgcgc
    30841 cgccggccat aattgcgagg ctgccgtcga tggccttcgg ctcgccgcca ctgaccgggg
    30901 cgtcaaggaa ctcgacaccc tttgccgcgc aggcggcgcc gaccttctga gacaccaggg
    30961 ggctgatcga actcatgtcg atcagcagag tgcctttccg tgcgccttcc agtactccac
    31021 cccggccgag aacagcccgc tccacgtcgg ggccgtcggg cagcatcgta atgacgatgt
    31081 ccgttcgtgc cgcaacgtcg cttgaggatt cgccgccagc cgctccggca gcgacgagct
    31141 cgcccacggg attgggcgcg atgtcataga cgacaagccg gtggcccgcc ttgaggagat
    31201 tcttcgacat gggcttgccc atgatgccga ggccgataaa gccgatattc gaggacaaga
    31261 gatcgctcct ttccgagttc agccagtaag tgcccattgt atgtgacgcg cgggccggcg
    31321 cggaaggcac gcggtctgcg atagagtcag gaaatgatgc tacgcggact tcttgtggga
    31381 ttgctgttca tagcggcgac tctggcggca gcagagccgc cagacgtgat ctattacaac
    31441 ggcaaggtga tcaccgtctg ggacgcgaaa ccgatcgccg aggctctcgc gatccgcggc
    31501 aaccggtttg catcagtcgg ctcgaacggc gaagttctga agaccgcggg cgccgggacg
    31561 aagaaaatcg acctgaaggg ccgcgctgtc atgccgggga tgatcgagag ccacacgcat
    31621 ccgatcggag ctgcgctgag cgagcaggac ggtcccatcc ccgtgatgaa ctcaattgcc
    31681 gagatccagg cccacatccg taaagaagcg gcgcggctgc ctccggatcg gctcattttc
    31741 gtcccgaaga tctacccatc caggctgaag gaacggcgtt acccgaaccg gtatgagatt
    31801 gacgaagccg ctccgggcag gctcgccatg tgcgacaacg gctatgccgg cgtcctcaac
    31861 tcggcgctgc tcgccaaagc cggcatcacg cgagacaccg aacagccgga gaacggcaag
    31921 atcatcaagg acgaacgggg cgagccgacc ggtctgattc ttggtgcgcc gcagatcctc
    31981 agtcccttcc gccgaagccg agagcggact cacgccgata tggtctgggc gatcaaggag
    32041 atgcagaagg cgtataacaa agccggcatc acgagcaccg ccgaccgggc ccagggtcca
    32101 gagggacttc gcgcttatca ggatgtgcac ggcaagggtg agatgacagt gcgtaccaac
    32161 gtcacttact acatcggcgc ccgaggcact ccgaaggacg tagccgcgga gatccggagc
    32221 atcccgttcg tgaccggatg gggcgacgat tggctgcgtg tcggaccgat caagacgacg
    32281 gtcgacgggg gtatcctcat cggcaccgct tatatgcgcg agccgtgggg gatgaacaca
    32341 atgatctacg ggtacgcgga tcccgactat cggggtatcc tcgccgtcaa gaaggaaaac
    32401 ctgttcgaga tggctcgcgt ggccaacgaa ctggggtggc agatgacggc gcacgccacc
    32461 ggaggcggcg cgatcgatct gctgctcgat gcctatgagg aggcggacaa ggtcaagccg
    32521 atccgcggac gccgctttaa cctgatgcac tcgaactttc cgaacgcgcg ggcgatcgag
    32581 cgggctaaga agctgggcgt catcttcgat agccagatcg cttggtacca ctgcgacgcc
    32641 gacgcgctaa aggacgtgtt cggcccggag cggatggcgc agttcctgcc gttccgctcg
    32701 ttgctggacg ccggagtggt ggttgtgggc ggttcggacc acatgatccg cttcgattcg
    32761 cgcgacgcca tcaacccgta tcacccgttc ttcgggatgt ggatggcggt gacgcggaaa
    32821 acggccgcag gcgccgttta caacccggag cagcgggtca ctcgggaaga agcgctgaag
    32881 atgtggacga tcaacggcgc ttacaactca ttcgaggaga acataaaggg gtcgattcag
    32941 ccgggaaagc tcgctgacat gatcgtcgtc accaaggatt accttacctg tcccgaggac
    33001 gagattagag atattgagcc gctgatgacc atggtcgacg gcaaggtcgc ctacggagga
    33061 ctgtagttat ttgccccgag acacctgcac gaccacctga ccgtcggatg ggagcgaaag
    33121 tcgtaacccg gagacgaggt cggcgccgag gtagaccacg ggagcgctgt cgccaacctg
    33181 cacacggtac ggacggtcgt gctcgacggt gaaccactcg ggccattcgt tcagcctcgg
    33241 ataattcacc gcaagattcc agtggtcccg gtggcgcgga tagtcgaaat acagacggcc
    33301 cgtccaggcg cgctcggtgg aaaggtagag gagcactccg ccgccatcgc tgaccgcacc
    33361 tacgtggacg cctgtgttcc agggcatcac ataagcgcct tgggtcttgc ggaacgcgta
    33421 catcagtgac gtacggatga agttgccgtc gccgtaccag tcctcgatga tgccgtcagg
    33481 cctctgacga tcgaaaaact ttcggaccgc aacgtcaagc gcctcgtcgg tttgcggatt
    33541 gggcagccgg ttcaacagga caagcgcgcc ttcgatgctg tctgagtacg cgttggagcc
    33601 ccatttacgg ccggcgccgg tttcgtcaaa gaggtatgcc ggcttgcggg tgacgccagc
    33661 gatggcatgg cggacggcgt cccgatactt ctcgtcgccc gtaatcatcc acgcggtgta
    33721 gatcgcattg aacatgtagc cccagcagtg ggcatgccgt gcgtcggaca cctttccgga
    33781 cgggacttcg acagacgtaa cccatactcc gtcagcgttg cggccgacct cgagaacgcg
    33841 gtcgatcatc cgcacgaagg gctcacggta ctgcttcgcc ttctcgggac gtttgtactg
    33901 cacgagaacg caggcttcgg agagcccgcc gacgatctcg ttgccgtggt ctgagaagac
    33961 gaacagggac tggatggcct ctcgcttttc caagttccag atgtgaaccg gcaggtaacc
    34021 cgtggctggg agcatctcga ggaagtagaa gtccgcaata gcgatcacct ggtccagata
    34081 cgtctcatcg cccgtcttcc aatacaggcg ggaaagaagc tgaagcatgt tgccgttaat
    34141 ttcggcggag ttcgacggca ggcggcccga gcgggatttg tacggggcgt tggcaatgat
    34201 gtcggaagca atgccccgca tgcggtgata ccagggggtc tcgccgagca gttccgtgat
    34261 gggaacaagt ccgtccttga cgtactcgct cgacccgaag atgatccggt cgagatcgac
    34321 agtcggatag acgaagccct tgccgcctgg ctggaggtcg tcagagaggc ggccgactcg
    34381 tgtggtctgt aaaacctccc ggcgcaggat gtcacgcatg gcgccatgat agagcggcgg
    34441 ttcggtaaag cgcgaagcca ggaccatgaa gggatagagg tcggcagccg aatcacgaac
    34501 gacccaatta ggattctcgc cggtccgggg gaggagtccg gtaacgggat cggcccgctt
    34561 gagccaggcg tgcatggtga cgttgctccg acgcagtgct tcggatgcct gcttcgcatt
    34621 gcgggcggcg atttccacga ggtcgcttgc cggtgtcttg gacgacgcca agccgagaaa
    34681 catcagggtg gtcaggattc cgcgcacgga tagtctccga attgggcctg ctgattgtaa
    34741 cagtttgggg ttgtacactg gtctggccat gaagctcaag gacagggtag cggtgatcac
    34801 cggagccggc gcgggcattg gccgggcgtg cgcgctcgag ttcgctaaag agggcgcatc
    34861 ggttgtggtt gcggacatcg acgccgccag cgcgcgggag acgacacggc agatcgccgc
    34921 tgccggcggg tgggcgctcg ccgtggagac ggatgtcgcg tccgcggatt ccgttcagcg
    34981 gcttgttacg gagactcgcg cagtctctgg aaggattcac gttctcgtca acaatgcggc
    35041 gatccaggtg aataagacgg tggaagacac gacggtcgac gagtggaacc gcgagatggc
    35101 ggtcaacgtc ggaggcgtat ttctttgctc taaattcttc gtgccgcacc tgcgggagac
    35161 gaagggcgcc atcgtcaaca tgtcctccgt gaacggtttc ttcgtcgagc cgatgtgcgc
    35221 cggctactgc gcgaccaagg cggcgatcat cgggctgacg aaggcgatgg cgatcgacca
    35281 cggtaaagac ggagtccggg tgaactgcat ctgtccggga tacatcgatg ccgggctggc
    35341 cgagggttat tttcaggcgc aacccgatcc ggcggcggcc cgcctggcgg cggggaagtt
    35401 gcacgcactg tggcgcatcg ggcggccgga ggaggtcggg cggctggcgg ttttcttggc
    35461 gagtgacgac gcttccttca tgacgggagc ggcggtggtg gtggacggag ggttcggctc
    35521 cggactgccg cccacggcat agggccacgc cgagaatgcg cccaaataga cgcaagtaca
    35581 cgaattttca atgggctgca gcttcattcg aaacgaagcc aatcccattt tcggacggtg
    35641 acaaccggat gagagcggcc gtcattctgg ctggcgggct gctggcggcc gctgccgtgg
    35701 cggccgttgt ttcatcggca gtgccactcg atgtcttgaa agaacggctg gacgcgatgg
    35761 ctcccgatgg aaccgcggac gagttcccgc tggagcggat cgccggaatc gtctggaaac
    35821 tgcgtctctt cggagtactg gcgttggggt cggctgtcgc tctgttggcc aagcgcaggc
    35881 aggtaagtca tgttctggcc ggggtgacgg aagccgccat cggcttcgcc gtaggcgtca
    35941 cgcggcagat attccgttgg gtcaagagcg aggaaaaggt tcatcttgcg gccctcggcc
    36001 tggtgttcct cgtgggtgtc gtggcgaggc tttggtttct ggggcagccg atgcgcgacg
    36061 atgaggcggc cacgttcctg gaattcggct cgaaaccttt cctttacgga atttccttct
    36121 atccggcgcc gaacaatcac atttttcaca ctgttctggt tcatgcatcg tgcctgctct
    36181 tcggcagcga gccctgggcc atccggctgc cgtcgtttct tgccggactg ttcctgattc
    36241 cagccgcgta ttgcactgcg cggatcttgt atgaccgcca cgtggcgctc cttacagtct
    36301 gcctcgtctc cgtttcttct ccgctgattc tctactcgac gaacgcccgc ggctacacga
    36361 tcgtctgcct tttgtttctc ttcacgctgg cagccgcgtt ccgattggtg ggcactcgag
    36421 ataaaggcgc gtggacctgg ctagtcatgc tgtccgccct cggtctcttc acgaccccgg
    36481 tcatgctatt cgcgacttgc ctcgggatgg tctggctggc attgtccggc tgggcggagc
    36541 accgccggag caaccgaccg aatgtcctgc cggagatcgt cttggcgctc gtggccgtgt
    36601 cgttgttgac cgtagtgttg tacacaccgg cgctagtcgc ttccggtgcg gcccaggtac
    36661 ttggaaacgg ggcattggcg ccttatccgt ggacggaatt tccaggccgg ctcagggatt
    36721 tcgctcattc cgtttggagt cagtggaaca tggatgtcag cccgttggtg cagtggcttt
    36781 gcctggcagg tgtggtcatt tcgctggtct tccaccggcg gctcgcccgg caccggattc
    36841 cactgatcct ggcggtgctt ctcgggtgcg tgccagtctt gatcgctcaa agatccgccc
    36901 gatacgcgcg cgtgtttctg tttctgctgc ctgtctactt gatgctggcc tcagccggga
    36961 tcagcgcgat gttccagtgg ctggaaagac gcaacaaatc cgcttcgtac ttgacgcttg
    37021 ccgtttctat gatcttcgtt ctatcgcagg cgggatacat catccatgcc gagactgtct
    37081 atcactcgga cgagaccggc acgctgcgca acgccgggca gattgcggca ttcttgcgcg
    37141 ggcgcctgcg cgatggcgat cgcgtgttgg cggatcgtgc gagtatgtac attctccggt
    37201 actacatcct ccagcaccac attcctccgg ggtatctgga cccgccgact gatcgctccg
    37261 ggaggcttat cgtcgtgtcg gggcgaaaat ccagcatcga cgctcgagat gtgctcgacc
    37321 ggcaacacat cgatatgccg gagccggtgg agacggtcat gctgcgcgag ttctccgaat
    37381 ccgtggtgta cgagatccgc gcagcgcggt gagcgtgttc cgcagacagc ggttgctata
    37441 cttttcttct tgtgttccat caacttgaga aacgtattga gcggagtgtc tccgcgcatc
    37501 tgaagacgag atacggcgtc gaggtggcgg tctccgtcga gcaaccgaag cagccggagt
    37561 tcggcgaatt ggccctgccg gtggccttcc aactcgccaa acagttgcgg caggcgccga
    37621 aggcgattgc cggtcagctc gcggcggagc taccggcggt ggaaggagtt gccgcactgg
    37681 aagtggccgg caacggatac ctcaatgtcc ggttcgaccg ggggacgtac ggggtcgcgc
    37741 tcgtgcgcgg cagcgcggat gccgagcggg cggcggcgga cgagaagatc atcgtcgagc
    37801 acaccaacat caacccgaac aaggcggcgc acatcgggca tctgcggaac gccgtgctgg
    37861 gcgacacgtt cgtgcggatg ctgagggcgg ggagacgtcg ggtcgaggtc cagaactaca
    37921 tcgacaacac gggcgtgcag gtcgccgatg tcgtggtcgg gtttcacttc ctcgagaaga
    37981 agacgccagc cgaggtcgcc gcgctgatcg cagaccggtc tcagaggttt gactatgtct
    38041 gttgggagct gtacgcgcgg gtatcagcat actaccatgg caatgccgag gcgctgaagt
    38101 ggcggcagga gacgctgcac gcgatcgagg cgggcgaggg agagctggcg gaactcggac
    38161 acctgatctc cgacgcgatc gtcgattgcc atctgaaaac gatgctacgc ctgggcgtcg
    38221 agtacgacgt gctgccccgc gaaagtgaaa tccttcacct gaagttctgg gcgacagcgt
    38281 tcgagctgtt gaaggaacgg aaagggatct acttcgagac cgagggaaag aacgcgggtt
    38341 gctgggtgat gccggctgcg gcgttttccg aaagcggcgg agacgaggac agcaaggtca
    38401 tcgtgcgctc aaacggcacg gtgacatacg tcggcaagga tatcgcgtac cagctttgga
    38461 agtttggcct gctcggcaag gactttcact accagccgtg gatgcagtat ccggacgggc
    38521 ggacgctgtg ggtatcgacc gatgagcctc ggaacgggaa cgtaccgcag ttcgggcatg
    38581 cctcctgggt gtacaacgtc atcgactcgc ggcaggcgta ccttcaggac gtcgtggccg
    38641 cgggactgcg agccctcgaa ttcaacgacc aggcggagcg ctcgatccac ttctcgtacg
    38701 agatggtggc gttgtctcca ggaacctgcg tggagatggg catcgagctt tccgaagaag
    38761 acaggaagcg gccgtatgtc gaggtgtccg ggaggaaggg actcggcgtg aaagccgacg
    38821 acctggtgga caagctgacc gaaaaagcgc tcgaggaggt ggattcccgg catcccgagg
    38881 cgccggtcga ggaacggcgg aaggtggcgg cgcagatcgc cgtgtgcgcg ctgcggtact
    38941 tcatgctgaa gtacacgcga aacgccgtga tcgctttcga tttcggcgag gcgctcagct
    39001 tcgagggcga gacgggaccg tatgtgcagt acgccgcggt acgagcgcgg aacatcctac
    39061 gaaagctcgc cgaaagaggc gagacactgc cggacttcgc gaaggatctc gatgaggcgg
    39121 caatgaagcg ccagcttgcg tcggaagatt tctggcagct cgtgctggcg atctcaaagg
    39181 cggattcggc tgtggagcgc gcgatcgcct caggggagcc ggcgcacgtg gcgaagtacg
    39241 ccttccacct tgcgcaggcg ttcaacaact tctatcacga gtatcctgtg ctgcacgagc
    39301 cggatcggga gcggaagacg ttccttttgt ggatgacgga ctacttccgt gcccaactgg
    39361 agcggactct ggaggtcctc ggaatcgaag taccggcgta tatgtaaacg gccgccgcgg
    39421 aggcgaaaaa gatccgagcg ctagactaga gatagggaga gagaagatgg cagtgaagaa
    39481 ttcctttggt gcggcggcga gcttgcgttc cggagagcgc tcgtatcgaa tccatcgcct
    39541 ggacgcgctg gagaagtcgg ggtatgggag catggcccgg ctgccggttt cgattcgggt
    39601 gttgctcgaa aacctgttgc ggcacgaaga tggggtgacc gtcaagtcgg aagacatcga
    39661 atacgtcgcg cgctggaacg gcaaaccgga gccgaaagag atcagtttca tgccggcgcg
    39721 ggtgctcttg caggacttta ccggcgttcc ggcggtggtg gacctcgcag ccatgcgcga
    39781 ggcgctgaaa gcaatgggcg ccgatccgaa actcgccaac ccgctgctgc cggcggacct
    39841 ggtgatcgat cactcggtac aggtggatca cttcggaacg ccggacgctt tccagttaaa
    39901 cgcatcgatc gagttccagc ggaaccggga gcgctatgag ttcctgcgtt ggggacagtc
    39961 ggcgttccgc aactttcgcg tggtaccgcc cgagacgggc atcgtgcacc aggtgaacct
    40021 ggagtacctg gcgccagtcg tgttttccgc gcaaaccaac ggcacggcgt cggcctaccc
    40081 cgataccctc gtggggaccg actcacacac aactatgatc aacggccttg gcgtagtggg
    40141 ctggggcgtc gggggcatcg aagccgaggc gtgcatgctc ggccagccgg tctccatgct
    40201 gctgccaccc gtggtgggat tcaaactgca cggcaagctg cgcgaaggtg taacggcgac
    40261 cgaccttgtg ctgactgtga ccgagatgct ccgtaagaag ggcgtcgtcg gcaagttcgt
    40321 cgagttctac gggcaagggc tgagcgccct gagcctcgcg gaccgggcga ccgtcgccaa
    40381 catggcgccg gagtacgggg caacgatcgg cttcttcccg gtggacgagg aaacgctgcg
    40441 ttatctgcgt ctcacgaacc gtccggcgga ggtgatcgcc ctggtagagg cgtacgtgaa
    40501 agaacaaggg atgttccgca cggactcgac gccggacccg gtgttctccg ataccttgga
    40561 tcttgacttg gcctcggtag tctcgtcgct ggccggaccg aagcggccgc aagaccggat
    40621 tgacttgcca aacatgaagc aaaacttcct gtcgggattg ggagcggagc cgaagaagac
    40681 gcaggtgcgt cttgacggcg ccgaggcgac ggtacaggac ggctcggtcg tcatcgctgc
    40741 gatcacgagc tgcacgaaca cgtcgaaccc gtcagtgctg atcgcggcgg gcctggtggc
    40801 gaaaaaagca gtggagcgcg gcctggagac gccgccctgg gtgaaaacaa gcctcgcgcc
    40861 gggatcgaaa gtcgtgatcg actatctgcg ggaggcgggc ctgctcgact atctggagcg
    40921 gctgaaattc caccttgtag ggttcggctg cacgacgtgc atcggcaaca gcggacccct
    40981 cccagagccg gtggcgcagg cagtgcagaa ggaaaagctc gtcgttgcgg cggtgttgag
    41041 cggcaaccgg aactttgagg gccgcataaa ctctctcgta aaggcgaact atctggcttc
    41101 gccgccgctg gtcgtggctt atgcgctcgc cgggcgggtg gacctggacc tacaaaacga
    41161 gccgctcggc acgggaaagg acgggcgccc agtctacctc cgggacattt ggccgtcaaa
    41221 cgaggaggtg cgggccgcgg tcggacagtt cgtgaacagg gagatgttcg gccggcagta
    41281 ctcggcggtc ttcgacggag acgagcagtg gaactcgatc cgggtgaccg gcggcgagat
    41341 ctacaagtgg gatgacgcct cgacgtacat caagaggccg ccctacttcg acacgccggt
    41401 cgatccggcg acctcagtcg gcaacctgcg aggcatgcgc gttctggcga tgctcgggga
    41461 ttccgtgacg accgaccaca tctcgccggc gggatccatt ccggtcgaaa gccccgcggg
    41521 gcaatacctg atcggactgg gcgttcagcc gaaggacttc aattcatacg gggcccggcg
    41581 cgggaatcac gaagtgatgg tgcgcgggac gcttgccaac atccggctca ggaaccagct
    41641 tgcgccgggg accgaaggcg gctggacggt gcacgtgccg tcgggcgaga agatgtggat
    41701 ctacgacgcc tccatgcgat accagaaaga gggcgtgccc ctgatgatcc tggcagggaa
    41761 ggagtacggc tccggctctt cacgcgactg ggcggcgaag ggtgtggcgc tgctcggcgt
    41821 gaaggcggtg atcgcggaaa gcttcgagcg gatccaccgc tcgaatctcg taggaatggg
    41881 cgtgctcccg cttgaattcc cggcgggcga gagccgggag tcgctcgggc tgacgggcac
    41941 ggagacattc cacatcgaag gcgtgaaaga agcagtgacc ggcgggcggc gagcaacggt
    42001 gcgggcggtg gccgcggacg gcagcgagaa gaccttccag gcggttgtgc gcgtggatac
    42061 gccccgcgaa gcggactact accggtatgg cgggattctg ccgtatgtgc tgcggcaact
    42121 ggcggccggg gcgtgagacg gccggtttgg acgtgcctgg cgctgacagc gctcgcgtcc
    42181 gtggcgttcg gccaggcgaa gtcctggcgc ggttgggaca cctaccacgt catcatgtgg
    42241 tccacgggcg ccccgaggga ctggggcacg tggatcaacc gcctgcgtga aatcggcttc
    42301 actgccgagg aactgacggc gggccgtgac accggtccgt tcacggtcaa caatttcggg
    42361 ttctacgtcg agaacctggt ttcggaactg gcctaccttc atagccggaa gccgctgtac
    42421 gac
//
LOCUS       DATIFQ010000347         5432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2413574, whole genome shotgun sequence.
ACCESSION   DATIFQ010000347 DATIFQ010000000
VERSION     DATIFQ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413574"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VLE22_16710"
     CDS             complement(<1..237)
                     /locus_tag="VLE22_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16097.1"
                     /translation="MTDGVSRREFLGVTGTLIAAKKTQAAGNDGEPWYRHIQRCGQTN
                     LNEKDLTGVDVEGWAEYWASLKVDVVLLNGGGIVA"
     gene            complement(259..1077)
                     /locus_tag="VLE22_16715"
     CDS             complement(259..1077)
                     /locus_tag="VLE22_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16098.1"
                     /translation="MDFLFGGISSITKGVLSVFAGRHPMFTLVPISVLAGVVMLWAFG
                     RFSNQAALRSTKKRLKASLYELRLFADDPGLIFRAQKELLIGNLRYMALMLRPAVILT
                     VPMVLLFVQMESFYGLSPLPVGGNGIVTARMTGGWDMSAPAPRLVPPDGIAVETPAVR
                     VLRDRTLVWRVRASREVSGQLRLAFAGGQIEKSIDSGAGPRYVSGRRVSSNASLFWHP
                     AEPRITGAPVEWVEVGYPGSEIEWFGVRLHWLIWFILISLVGALLFKGRMKVTF"
     gene            complement(1090..1287)
                     /locus_tag="VLE22_16720"
     CDS             complement(1090..1287)
                     /locus_tag="VLE22_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16099.1"
                     /translation="MISKASVKLNKALWDKVKRCADAGGYSSPQEFVEHVLEREVARI
                     LDDAESKEEIVKKLKGLGYLE"
     gene            complement(1491..3404)
                     /locus_tag="VLE22_16725"
     CDS             complement(1491..3404)
                     /locus_tag="VLE22_16725"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSB16100.1"
                     /translation="MRRPQWRTWLLPALLVALIAAGCGRSQSKTAVPKIIVLGMDAMD
                     PVFLERHWDALPNLDRLRHSGEFRRLGTSIPPQSPVAWSTFITGMDPGGHGIFDFIHR
                     NPKTLMPFSSMAETEEPKRTLTIGPYVIPLSQGKVKTFRQGKAFWQMLDEQGIPVTVI
                     RIPNNFPPVECNGRTLSGMGVPDMRGTFGTFSYYSDDPNERGRNVSGGRILKIRTEGH
                     HAILNVEGPVNTLRKDRPMSSVAVEVDRDPAAPAAQFSVGDERIVLREGEWSNWIRVE
                     FPLIPGLTGASGMFRIYAKQIHPHLGIYVSPVNLDPEKPDLPISTPKGYSAELARAIG
                     PFYTQGMAEDTAAFRQGVLTREEYRRQSHMVSREHLSLLDYEVQRFTGGLLFFHFFGI
                     DQDSHVFWGTHEKELLETYKLADEAVGRVMKKAGDATLIVMSDHGFSNFNRAVHLNTW
                     LWREGFLELDDPENASDEELFVHVDWQRTMAYSIGLNSVYLNLEGRERNGIVTKGEES
                     ASILQRLKERLEGFRDPDNGRPVVERVYLTSEVYHGAAAESAPDMIVGWAPGYRSSWQ
                     TALGAVPKVLIEDNKDEWAGDHCIAAESVPGSFLCNRKTRLADPRLQDVTVTLLAEFG
                     LSKGKGMSGRAMF"
     gene            complement(3401..>5432)
                     /locus_tag="VLE22_16730"
     CDS             complement(3401..>5432)
                     /locus_tag="VLE22_16730"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSB16101.1"
                     /translation="IGPGAGFAFLGSFLTLLTGFVLSAASLLLWPFRMMRRTLRRRKG
                     FRHAKVKRVVFLGLDGLDPDITERLMAEGRLPNLARLREEGSYRRLRTTFPSLSPVAW
                     STFSTGVSPARHNIFDFLNRNLKSYLPELSSARVEKPRRVLRLGRLRIPLSRPNVELR
                     RKSQPFWKILGEHGVGSTVLRVPITFPPDKFDGRMLSAMCTPDLKGTQGSFSQFTTRL
                     EKATFENGSRYPLRPADGAFEGHIEGPEDLFHEGGDVLRIPFRLQVDRAKKSARLEID
                     GESHKLSLGEYTPWIRLTFRTALRVKVTGIARFLLNEVEPEVSLYLSPINIDPENPAL
                     PISQPSYYAIYLANLLGPYATVGMAEDTWALNESVIDEEAFLKQAYLTFQEREAMFLS
                     ALEKTRSGVVACVFDTSDRVQHMFFRYLGGDGPEAPAAGQHAGTIEEMYERMDRLVGK
                     AMANAGRDTALFVLSDHGFCSFRRGVNLNSWLLRNGYLALKDGATESGPYFQGVDWSR
                     TRAYSLGLAGMYLNLKGREAQGTVAPGAEAEALKQEIAGRLGQLHDEDRSRAAIREVY
                     VTNSLYRGPYLDAAPDLIVGYHRGYRISWGAAVGRVTPEVIEDNPKAWSGDHCVDPVL
                     VPGVLFSNRKIDAEDPGIEDLAPTALKLFGIDPPAWMEGKPVFRFAPSGGDL"
BASE COUNT          969 a         1878 c         1580 g         1005 t
ORIGIN      
        1 ggcgacgatt cctcccccgt ttagcaggac gacgtcgact ttcaggctcg cccagtactc
       61 ggcccacccc tcgacgtcca cacctgtcag atccttctcg ttcaggttgg tttggccgca
      121 gcgttgaatg tgccggtacc acggctctcc gtcattcccc gcagcctggg tcttctttgc
      181 cgcaatcaat gttcccgtga cgcccagaaa ctctcgccgc gacacgccgt ccgtcatgac
      241 tccttccctt ccgtcgcctt agaacgtcac cttcatgcgg cctttgaaca acaaggcccc
      301 gaccagagag atcaggatga accaaatcag ccagtggagg cgcacgccga accactcgat
      361 ttcggagcca ggatagccaa cctcaaccca ttcaaccggg gcgccggtga tccgaggttc
      421 cgccgggtgc cagaacaggg acgcgttcga actgacacgc ctcccggaaa cgtagcgggg
      481 gcccgccccc gagtcgatgg atttctcaat ctgaccgcca gcgaatgcta aacggagctg
      541 gccggagacc tctcgcgacg ctcgtacccg ccagacaagc gttcggtcac gaagcacgcg
      601 cacggccggt gtctccacag cgatcccatc aggcggtacc agacggggcg ccggtgcgct
      661 catgtcccat ccgccagtca ttcgggcggt cacgataccg ttcccgccca ccggaagcgg
      721 cgacagcccg tagaacgatt ccatctgcac gaacagcaac accatgggga ccgtgagaat
      781 cactgcggga cgcagcatta gcgccatgta ccggaggttg ccgatcaaca actccttctg
      841 ggcgcgaaag atcaggccag ggtcgtcggc gaacagccgc agttcgtaga ggctcgcctt
      901 caggcgcttc tttgtgctcc gcagggccgc ctggttcgaa aaacgcccga aagcccacag
      961 catcacgacg cccgcgagca cgctgatcgg aaccagcgtg aacatcgggt gccgccctgc
     1021 aaacaccgac agcaccccct tggttatcga gctgatcccg ccgaacagga aatccacgtt
     1081 gtgtccgttc tactccaggt agccgaggcc cttcagcttc ttgacgatct cttccttgga
     1141 ctccgcatcg tcgagaatgc gcgccacttc cctctccagc acgtgctcga cgaactcctg
     1201 tggcgagctg tacccgcccg cgtccgcgca gcgcttgacc ttgtcccaaa gcgccttgtt
     1261 cagcttcaca ctcgccttcg aaatcatgat ctcgttccct cagcagggga caggcttact
     1321 ctgccccgag cgcagttctg tgtgggggac aggcctactc cgccccgagc gcagttctgt
     1381 gtgggggaca ggcttactct gccccaagcg cagttctgtg tggggacagg cctactctgc
     1441 accaagcgca gtttgcgagc gggcagagct gcctgtcccc catgccttct tcaaaacatc
     1501 gccctcccgc tcatgccttt ccctttcgac agcccgaact ccgcgagcag cgtcaccgtc
     1561 acgtcctgca agcgtgggtc ggccagccgc gttttccggt tgcacagaaa cgatcctgga
     1621 acgctctccg cggcgatgca gtggtcccct gcccattcgt ccttgttgtc ctcgatcagc
     1681 accttgggca ctgcgccaag cgccgtctgc caggaagaac ggtaccccgg cgcccagccg
     1741 acaatcatgt ccggcgcgga ctccgccgct gcgccgtggt acacctcgct ggtcagatac
     1801 acccgctcga ccaccgggcg tccgttgtct gggtcgcgga agccctccag ccgctccttg
     1861 aggcgctgga gaatgcttgc gctttcctcg cccttagtca cgatcccatt gcgctcgcgg
     1921 ccttccaggt tcagatagac ggagttgagt ccgatggaat acgccatcgt ccgctgccag
     1981 tccacgtgta cgaacagttc ctcgtcgctc gcgttctccg gatcgtccag ctcgagaaaa
     2041 ccctcgcgcc aaagccacgt gttcaggtga acggcccggt tgaaattgct gaagccgtgg
     2101 tcggacatca cgatcaaagt tgcatcgccc gccttcttca tcacgcggcc gactgcctca
     2161 tcggcgagct tgtaggtctc gagcagttcc ttctcgtgcg tcccccagaa gacgtgcgag
     2221 tcctggtcga tcccgaaaaa gtgaaagaac agcaggccgc ccgtgaaccg ttgcacttcg
     2281 taatcgagca ggctcaggtg ttcccgcgac accatgtggc tctggcggcg atattcctct
     2341 cgcgtgagca cgccttgccg gaacgcggcg gtgtcttccg ccatcccctg cgtgtagaac
     2401 gggccgatcg cccgtgcgag ctcggcgctg tagcctttcg gcgtcgagat cggaaggtcc
     2461 ggcttctctg gatcgagatt caccggcgaa acgtaaatcc cgaggtgcgg atggatctgc
     2521 ttggcgtaaa tgcggaacat gccggaggcg ccggtcagcc cgggaatgag cgggaactcc
     2581 actcgaatcc agttcgacca ctcaccctcc cgcagcacga tccgctcgtc gcccacgctg
     2641 aactgagccg ccggcgccgc cgggtcccgg tccacctcta cggcgacgct ggacattggc
     2701 cggtccttcc gcagtgtgtt caccgggcct tcgacgttca ggatcgcatg gtgtccctcc
     2761 gtgcggatct tgaggatgcg tccacccgaa acgttgcgcc ctctctcgtt cggatcgtcg
     2821 ctgtaatacg agaaggttcc gaacgtccct cgcatgtcgg gcacgcccat gcctgagagc
     2881 gtccgcccgt tgcactccac cggcggaaag ttgttcggaa tccggatcac cgtaacgggg
     2941 atgccctgct cgtccagcat ctgccagaac gccttgccct gccggaaggt cttcaccttg
     3001 ccttgggaaa gcgggatgac gtagggtccg atcgtcaggg tccgcttggg ttcctcggtt
     3061 tccgccatcg acgagaatgg catcagcgtc ttcggattgc ggtggatgaa gtcgaagatc
     3121 ccgtggccgc ccgggtccat gcccgtgatg aacgtagacc acgccaccgg gctttgcggc
     3181 ggaatcgatg tgccgagtcg ccggaattct ccgctgtgtc gaagccggtc caagttcggc
     3241 agcgcgtccc aatgccgctc cagaaacacc gggtccatcg catccatgcc gagcacgatg
     3301 atcttgggta cggccgtttt ggactggctc ctgccgcacc cggctgcgat cagagcaaca
     3361 agaagcgcgg ggagcagcca cgtccgccac tgcggccttc tcataggtcg ccgcccgacg
     3421 gtgcgaaacg gaaaaccggc ttgccctcca tccacgccgg cggatcgatg ccgaagagct
     3481 tcagggcggt cggcgcgaga tcctcgatgc ccggatcctc cgcgtcgatc ttccggttcg
     3541 aaaacagcac acccggcacg agcacaggat ccacgcagtg gtccccgctc cacgccttcg
     3601 ggttgtcctc gatcacttcc ggcgtcaccc ggccgacggc ggccccccag gagatccggt
     3661 aaccccggtg gtagccgacg atcaggtccg gcgccgcgtc gagataagga ccgcgataca
     3721 acgagttcgt cacgtagact tcccggatcg ctgcgcggct ccgatcttcg tcgtggagtt
     3781 gcccgagccg cccggcgatc tcctgcttca gggcttccgc ttccgcgccc ggagccacag
     3841 tcccttgcgc ctcgcgtccc ttgaggttca gatacatccc ggccaggccc agcgagtacg
     3901 cccgcgtgcg gctccagtcc acgccctgga agtatgggcc gctctccgtg gcgccgtcct
     3961 tcaacgctag gtaaccgttc cgcagcagcc aggaattgag attcacccca cgccggaacg
     4021 agcagaatcc gtggtccgat agcacgaaca aggccgtgtc gcggcccgcg ttggccatgg
     4081 ccttcccgac gagcctgtcc atccgctcgt acatctcctc gatcgttccg gcgtgctgcc
     4141 ctgccgccgg cgcctccggc ccgtcaccac cgaggtagcg gaaaaacatg tgctgcacgc
     4201 ggtcgctcgt atcgaagacg caggccacca cgccgctgcg cgtcttctcg agggcgctca
     4261 ggaacatcgc ctcgcgctcc tggaacgtca gataggcctg cttgagaaac gcctcctcgt
     4321 cgatcacgct ttcattaaga gcccatgtgt cttccgccat cccgacggtg gcgtacggcc
     4381 caagcaggtt ggcgagatag atggcgtagt aggaaggctg gctgatcggc agcgccgggt
     4441 tttccgggtc gatgttgatc ggcgagaggt acagcgatac ttccggctcc acctcgttca
     4501 agaggaaacg cgcgatcccc gtcaccttca cccggagagc cgtccggaac gtcagccgga
     4561 tccatggcgt gtactctccc agcgacagtt tgtgcgactc gccgtcgatc tcgagccggg
     4621 cgctcttttt cgcccggtcc acctggagtc gaaacgggat gcgcagcacg tcaccgccct
     4681 cgtggaacag atcctcgggc ccttcgatgt ggccttcgaa ggcgccgtcc gccgggcgaa
     4741 gcggataccg gctcccgttc tcaaacgtcg ctttctcgag ccgcgtggtg aactgcgaga
     4801 agcttccctg cgtccccttc aggtcgggcg tgcacatcgc cgacagcatc cgtccatcga
     4861 acttgtccgg cgggaaagtg atcggtactc gcaggaccgt gctgccgacg ccgtgctccc
     4921 cgaggatctt ccagaacggc tggctcttgc gccgcagttc cacgttcggg cgcgacagcg
     4981 gaatccgcaa acggcccagc ctcagcaccc ggcgcggttt ctccactcgc gccgaggaaa
     5041 gctccggcag atacgacttc agattccggt tcagaaaatc gaagatgttg tgccgtgccg
     5101 ggctgacccc ggtcgagaac gtcgaccaag ccaccgggga tagggacgga aacgtcgtcc
     5161 gcaggcgccg gtacgaccct tcctcccgga ggcgggcgag gttcggcagc cttccctccg
     5221 ccatcagccg ctccgtgata tccggatcga gcccgtcgag cccgaggaac accacacgct
     5281 tcactttggc gtgccggaag cctttccgcc tgcggagggt gcgccgcatc attcggaaag
     5341 gccacaacag tagggaggcg gcgctcagca cgaatccggt caggagagtc agaaacgaac
     5401 ccaggaacgc gaagcccgcc cccggcccga tg
//
LOCUS       DATIFQ010000348         5937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1455477, whole genome shotgun sequence.
ACCESSION   DATIFQ010000348 DATIFQ010000000
VERSION     DATIFQ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455477"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VLE22_16735"
     CDS             complement(<1..410)
                     /locus_tag="VLE22_16735"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HSB16102.1"
                     /translation="MFLEQADCERIHGAALDVLDDVGVRIDDPDVVKLLADAGAVVKD
                     STVVRIPQRLVEWALTQSPKSARFADRRGNLFDICSGGDTLVLTGNALYITRGRTRTD
                     LKSADFADLARIVDACPNIHGMVGTSIADIPPTCR"
     gene            461..3766
                     /locus_tag="VLE22_16740"
     CDS             461..3766
                     /locus_tag="VLE22_16740"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HSB16103.1"
                     /translation="MKRKVVISLLIGVLAGWCAAPQQEGGSAVKAYEGTIDIPTHQPS
                     GREVQPPLFPSSTVGGLYPFPPFILPVKPDGPWPKTYQAVFVENEYLKLTYLPDLGGR
                     FFALYDKVRKREVFYRNDVIKPSHYNSKLSWPQSGIELTGPYDTHMLTFYGEPFWSHR
                     VVRHEDGSVSLVLGSIDPVYHMKVDLSARLYPGLSAMQISVFCYNAREGRMPHMFWIS
                     SAVPARPKTRFIYPMTRTIGHTTSEVGDWPVFNGNDYSWDRNNQHMLGVFGIDIYDDF
                     QGAYDFETDYGTFRHADRRVVQGMKMWTFGHSDRTPALTHGYTDNAGPYVEVQSGRYV
                     WDGFYEWLAPHKYENWSEWWFPVAGIGGFTTTSRDVALNLDVVPDPQGSKSTVKVGLS
                     ANRAMPGARITVTATHGEILNTTADLAPATPFNRSVGVSAGAGGLRQLVVRVSEASGN
                     EVLHYTRPDEDPGRNEYTPFTRPLERPQKDPKDMSVEELVLAAEGKLKQLNHSGAVEL
                     LNQALARDPGYSHAHLLFGMLYFNTVRYAEAVKHLEKAIDRDPYIYEAHYYLSMSQLK
                     LGDTRSAERNLYFIPPDSLYFPNREYHLGRLEFLRGSPEKARSHLEKAVMVNGYYLDA
                     RLLLALIDREQGKKESALTRLAEVESIDPTSQWAAAERYFLAKDGAARQELLQLMGGQ
                     AQEAIDVSLFYRRLNRWAEAAEILGLVEQNNHDVWGTPSEFYYTLAYLMNKLGDGKAA
                     AGYREKARAAAENIDRYPYREESEAPLAEAVEKDPRDAVARFYLACLQYHLGRAEDAI
                     RNWEGAVEASPADFRMRRALGMAYAEQGQPVEKAAAQLEKAVELRPEHVRTMNDLSAI
                     YAKAGRFDEQLAMLDRALRRTPEDDNLAEGVLTANLMTGRYAEADKVVATHKFEPRHR
                     TYGLRDKYRYLRYGMGAEAFHAGRYEEALKLFRSALTPPASLGVDDFQFESTPRVNYY
                     IGRALEAMGKRDEARTAYQRAASGYQQLSGDQDSWNAENFHMVLALARLGNKEAAAKL
                     EREFEEYAKTQMESQRRQRQTQARYLLGLVKKYRGEIAEARRLMEEALKLEPDFLGPR
                     LELRGDVVDKLGS"
     gene            3784..5142
                     /locus_tag="VLE22_16745"
     CDS             3784..5142
                     /locus_tag="VLE22_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB16104.1"
                     /translation="MKRLAHTRRCFLEAAPALAGGWRLLAAEDDRPAVRDPRATVGDD
                     VVAPKWDTGIEITVGPDKADVVGSTGKAIQAAVDYAARWGGGTVRVLPGTYQLRNAVF
                     LQSGVRLSGSGPDTVLVKGPSVRTKLAVDADFYEQEVTLADPKGFEVGDGVCFRARSQ
                     RHEGVDIFTRTLVARSGSRFKLDRPLPGYPGISDLWLEGDASAATLFALIHGEGITDA
                     AVENITLDGNKANNEFLNGNHTGCIYLLSSNRVTIRRVTARNFNGDGISWQTSHDVTV
                     EECRSQDNAGLGLHPGSGAKRPTARRNRLERNDIGFYFCWGVKWGVVEDNVILDSKRF
                     GIHFGHNDTDNIARRNEIRNSGRAGVIFIPGDGRVAPPNYCRLEANRILDNGGENGIG
                     VDVQGTATGLVIARNEILETRVPAKRTGVKIAAEVGEVKLIENKIEGFSIPVSDLRRT
                     AG"
     gene            5426..5860
                     /locus_tag="VLE22_16750"
     CDS             5426..5860
                     /locus_tag="VLE22_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16105.1"
                     /translation="MPNNHSSKRIMIAAGLLALLSVADVYAETKLVRVEIPFRFIAAD
                     QVLPAGVYRVTVDPTFNRMELRTEDGASGAFVSVYTAAKAPASDTGKLLFHGYGNTFF
                     LQGVWAAGMTSGYKLPASKAEREMAKVRSAGAQVAWLPIPAR"
BASE COUNT         1205 a         1737 c         1958 g         1037 t
ORIGIN      
        1 cggcacgtcg gagggatgtc ggcgatgctg gtgccgacca ttccgtggat attcgggcag
       61 gcgtccacga tgcgtgccag gtcggcgaag tccgccgact tcagatcggt gcgcgtgcgg
      121 ccgcgggtga tgtagagtgc gttgccggtc aggacgagcg tatcgccgcc ggaacatatg
      181 tcgaagagat tgccgcggcg gtccgcgaaa cgcgctgatt tgggggactg cgtgagcgcc
      241 cactcgacca gacgttgcgg gatgcggacg acggtcgagt cctttaccac ggcgccggcg
      301 tccgccagca acttcacgac atcgggatcg tcgatacgca cgccgacgtc atcaagaacg
      361 tccaatgccg ccccatggat gcgttcgcag tctgcttgtt caagaaacat gtcgaagaac
      421 cagcatatta tggtttctta gcgcggggtt ccggacaaaa atgaaacgta aggttgtcat
      481 atctctgctc atcggtgtgc tggccgggtg gtgcgcggcg cctcaacagg agggtgggtc
      541 cgccgtcaag gcgtatgagg gcacgatcga tattcccacg caccagccgt cgggcagaga
      601 ggtgcagcca ccgctcttcc cgagctcaac cgtgggcggg ctttacccgt ttccaccgtt
      661 catcttgccg gtgaagcccg acggcccgtg gcctaagact taccaggcag tctttgtcga
      721 aaacgaatat ctcaaactga cctacctgcc cgacctggga ggccgtttct tcgctttata
      781 cgacaaggtc cgcaagcgag aggtcttcta ccggaacgat gtcatcaagc cgagccatta
      841 caactccaag ctgagctggc cgcagtccgg catcgagctg acgggaccgt acgacacaca
      901 catgctgacg ttttacggcg agcctttctg gtcgcacaga gtggtacggc acgaggacgg
      961 cagcgtgtcg ctggtgctgg gctcgatcga cccggtctac cacatgaagg tggacctgtc
     1021 ggcgcggctc tatccgggcc tgtccgcgat gcaaatcagc gtcttctgct acaacgcgcg
     1081 cgaaggccgg atgccgcaca tgttttggat cagctcagcc gtcccggcgc gcccgaagac
     1141 acgcttcatc tacccgatga cgaggacgat cgggcacacc acgtcggagg tgggcgactg
     1201 gccggtcttc aatgggaacg actacagttg ggaccgcaat aaccagcaca tgctgggcgt
     1261 cttcggcatc gatatctacg acgatttcca gggcgcttac gatttcgaga ccgattacgg
     1321 gacgttccgc cacgccgacc ggcgagtcgt gcagggcatg aagatgtgga ccttcggcca
     1381 ctcggaccgc acgccggcgc tgacccacgg gtacacggac aatgcgggac cctacgttga
     1441 ggtgcagagt gggcggtatg tctgggatgg tttctacgag tggctcgcgc cgcacaagta
     1501 cgagaactgg agcgagtggt ggtttccggt ggcaggcatc ggcggtttca cgacgacttc
     1561 gcgcgacgtg gccctgaatc ttgatgtcgt tccggatccg cagggaagca agtccacggt
     1621 caaggtcggc ctttccgcta accgcgccat gccgggggcg cggattacgg tgaccgcaac
     1681 gcacggcgaa attcttaaca cgacggcgga tctcgcgccg gcgacgccgt tcaaccgttc
     1741 ggtcggcgtc agtgcaggcg cggggggact gaggcagttg gtcgtccggg tcagcgaggc
     1801 atccggcaac gaagtgctgc actacacgcg gcccgacgaa gatcccggcc gcaacgagta
     1861 cacgccgttt acgcggccgc tcgaacggcc tcagaaagac ccaaaggaca tgagcgtcga
     1921 ggaactggtg ctggccgccg agggaaagct gaaacaactc aaccatagcg gcgcggtaga
     1981 actgctgaat caggcgctcg cgcgcgaccc ggggtactcg cacgcccatc tccttttcgg
     2041 aatgctctat ttcaacaccg tgcgttacgc cgaggccgta aagcatctgg agaaagcgat
     2101 tgatcgcgac ccctatatct acgaggctca ctactacctg tcaatgagcc agttgaagct
     2161 cggcgatacg aggagcgcgg aacggaatct ttacttcatt ccccccgact cgctgtattt
     2221 cccgaaccgg gagtaccacc tcggccgcct cgagttcctg cgtgggtcac cggaaaaggc
     2281 gcgctcgcac ctggaaaagg cggtcatggt gaacggctac tatctcgacg cacggctgct
     2341 actcgcgttg atcgaccggg agcaagggaa gaaagaaagt gcgctgactc gactggctga
     2401 ggtcgagtcg atcgatccga caagccagtg ggcggcggcc gaacggtact tcctggcgaa
     2461 ggacggggcg gcgcggcagg aactgttgca gttgatgggc gggcaggcgc aagaggcgat
     2521 cgacgtatcg cttttctatc gccgattgaa tcgctgggca gaagcggccg agattctggg
     2581 ccttgtcgag cagaacaacc atgacgtgtg gggcacgccg tctgagttct actacaccct
     2641 tgcgtacttg atgaacaagc tcggtgacgg caaggctgcg gctggctacc gggagaaggc
     2701 ccgggccgcc gctgagaaca tcgaccgcta tccgtaccgc gaggaaagtg aggcgccgct
     2761 tgcagaagcc gtggagaagg accccaggga cgccgtggcg cgtttctatc tcgcctgtct
     2821 ccaatatcac ctcggacgcg ccgaagacgc gattcgaaac tgggaggggg cggtggaggc
     2881 aagccccgcg gactttcgga tgcgacgggc gctcgggatg gcgtacgcag aacagggtca
     2941 gccggtggag aaggcggcgg cgcaactgga aaaagcggtc gagctgaggc ccgagcacgt
     3001 tcggacgatg aacgacctca gcgcgatata cgcgaaagcc ggccggttcg acgagcagct
     3061 tgccatgctg gacagagcgc tccggcggac cccggaggac gacaacctgg ccgagggagt
     3121 ccttaccgcc aatctgatga cgggccgcta tgccgaggcg gacaaggtgg tggcgaccca
     3181 taaattcgag ccacggcacc gcacctatgg gctgcgggac aagtatcgct atctgcggta
     3241 cgggatgggc gcggaggcgt tccatgccgg ccgctacgag gaagcgttga agttgttccg
     3301 gtcggcgctg acgccgcctg cttcgttggg tgtggacgat ttccagttcg aatcgacgcc
     3361 gcgcgtgaat tactacatcg gccgcgcgct ggaagcgatg gggaagcggg acgaggctcg
     3421 gacagcctac caacgcgccg cttccggcta tcagcaactc tcgggcgacc aggacagttg
     3481 gaatgccgaa aacttccaca tggtactggc gctggcccgg ttagggaaca aggaggccgc
     3541 ggcgaagctg gaaagagagt tcgaagagta tgcgaagacg cagatggagt cgcagcggcg
     3601 gcagcggcag acgcaggcgc gctatctgct cggactcgtg aagaagtacc ggggagagat
     3661 cgctgaagcg cgccgtctga tggaagaggc gctgaagctg gagccggact tccttgggcc
     3721 gcgtctggaa ttgcgtggcg acgtcgtgga caaactcggc tcttgagcag gcaggaggtt
     3781 gagatgaaaa gactcgctca tactaggcgt tgttttctcg aagctgcgcc ggccctggcg
     3841 ggcggatggc ggctccttgc ggcagaggac gaccgtccgg cggtgagaga cccaagggct
     3901 acggtcggcg acgatgtcgt cgcgcccaag tgggacacgg gaatcgagat tacagtgggg
     3961 ccggacaagg ccgacgtcgt gggttccacg gggaaggcga tccaggcggc ggtggactat
     4021 gcggcgcgat ggggcggggg aacggtgcgt gtgttgcccg ggacctacca gcttcggaac
     4081 gcggtatttc tgcagtccgg cgtgcggctg tccggcagcg gtcctgacac ggtgctggtg
     4141 aagggcccgt cggtgcggac caagcttgcc gtcgacgcgg acttctatga gcaggaagtc
     4201 acccttgccg acccgaaagg cttcgaagtt ggcgatggcg tgtgcttccg ggcgcgaagc
     4261 cagcggcacg agggagtcga catcttcacc cgcacgctgg tcgcgcgtag tggcagccgc
     4321 ttcaagctgg accggcctct cccgggttac cccggcatct ccgacctgtg gctggagggc
     4381 gatgccagcg ccgccactct tttcgctctc atccacggcg aggggatcac cgatgccgcg
     4441 gtcgagaaca tcacgctcga cgggaacaag gcgaacaacg agtttctgaa cggcaatcac
     4501 accggctgca tctacctgct gagcagtaac cgcgtcacga tccggagggt tacggcgcgc
     4561 aatttcaatg gtgacggcat ctcgtggcag acaagccacg acgtcacagt cgaagaatgc
     4621 cgttcgcagg acaacgccgg gcttgggttg caccccggct ccggcgcgaa acggccgacg
     4681 gcgcggagga accggctgga gcggaacgac atcggcttct acttctgctg gggtgtgaaa
     4741 tggggcgttg tagaggataa cgtcatcctt gacagcaagc ggttcgggat acacttcggc
     4801 cacaacgaca ccgacaacat cgcacgccgt aacgagatcc gcaacagcgg gcgagccggc
     4861 gtcatcttca tccccgggga cggccgcgtg gctccgccaa attactgccg gctggaggcg
     4921 aaccgcatcc tcgacaacgg aggagagaac ggaattggcg tcgatgttca gggaacagcc
     4981 accggccttg tcatcgcccg caacgagatc ctcgaaaccc gagttcccgc gaaacggacc
     5041 ggcgtcaaga ttgcggccga ggtcggggaa gtgaaactga tagagaacaa gatcgaaggc
     5101 ttctccattc ccgtcagcga tctgcgacga acagcgggct agcaggaaac cgaagcgtac
     5161 taggcgtgta agagtattcc taccgattgc cccgactggc agtcgctgct ttcaccaaag
     5221 tgccgcgctt tgacgagtgg aaggtgatca accgcttccc ctgcaaacag ggagttcccc
     5281 gtggctccag gcggcacaag tccacaccag cttgtcattg cacgccaaag tgcgccagcc
     5341 gtggtgccga caatctctta cgatctccaa ggggaggaaa gagccgtatt ctcggggtgg
     5401 ctgaagaggc cggaaagaga acgcaatgcc aaacaatcac tcctcgaaaa gaatcatgat
     5461 cgcggctggg ctccttgccc tgctgtcggt ggccgatgtt tacgccgaaa ccaagctggt
     5521 ccgggtcgag atcccgttca gattcatcgc ggcagaccag gtcctcccgg ccggagtgta
     5581 ccgggtgacg gtcgatccca ccttcaaccg gatggaactg agaaccgagg acggcgcctc
     5641 cggcgcgttt gtctcggtct acacagctgc gaaagcgccc gcttctgaca cgggcaaact
     5701 gctgttccat ggttacggca acaccttctt cctccaaggc gtatgggctg ccggaatgac
     5761 cagcggatat aagctacccg cttcgaaggc ggagcgcgag atggcgaaag ttcgctccgc
     5821 gggcgctcag gttgcctggc tacccatccc tgcgcggtag attgactgcc tttgctcggt
     5881 ttcttggttg ggcccggtga gagcgtgccg gtcggcaagc cggcacgctc ttcgcct
//
LOCUS       DATIFQ010000349         4165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2798496, whole genome shotgun sequence.
ACCESSION   DATIFQ010000349 DATIFQ010000000
VERSION     DATIFQ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2798496"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VLE22_16755"
     CDS             <1..282
                     /locus_tag="VLE22_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16106.1"
                     /translation="GNREYACELVLREMERLPNVPDPASAYTDLSCSDLPASVQVDAS
                     SGLDADGKLFSRVQVRYETVQLIPIPGLLTGKTTITRTAESRMYGKTRP"
     gene            285..836
                     /locus_tag="VLE22_16760"
     CDS             285..836
                     /locus_tag="VLE22_16760"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HSB16107.1"
                     /translation="MEPSKKQRGSTLLEFTLVGIPVIFMLISIFEISRGMWLYHTLAS
                     AIKDGTRFAIVHGNNCNIYPSNCVVTIRDISERIRRNAVGFVPDDLQNLEFISATRGR
                     FTCATLAACLDTGNPGDLSWPGGPVGSAPEDFPDGRQFAWVEIRAQYPFRSAIAMFWP
                     GAGRGQNFGEFMLPASSRDRIQY"
     gene            848..2680
                     /locus_tag="VLE22_16765"
     CDS             848..2680
                     /locus_tag="VLE22_16765"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HSB16108.1"
                     /translation="MAKNLGKRRGQVLIMVTLALLMLCGMLGLVVDLGWSYFTKKSAQ
                     AAADAGALAAVKAAMDDAVDISSYTCGGGGAECLSVPTPCPNLPGNLQSACMYAQQHG
                     FTTGGRQLVTVQAWDRTPNPTVVENCGQGGASVAHPPTADPPCVDTFYWVTVRVTERI
                     PQLFSSVFGNTEGIVSARATAAVAKSVTPASLILINRENDPWTDDSGQVYTGNNLYVG
                     GTPQVIVPGGIIMASGAGGPSKQDAWAGYIAGSGTVDSQGGTYIRDNGDYYISGGGSW
                     TITPENQPDNEIFWDPYEGIAQPPVASNPLTPIPVPNGNLGSLKNTVCKSGCPSGNYF
                     ATGIPKGCNNCAEVATGEQIVINTDIEFNGGGFGDFNFFGGLNIGQAKVTFGPGRYVM
                     AGTLNENIPLFNTDNKAKIIGGNGGDAGRLFILTDSQYPGLETQVASISYTREWPGPN
                     NSLTFSKSSIKSGNNEGSSTDLYSLSKDIGNTVPELAPYQGFLLWQDRRNSYVHYKDA
                     YKYVDDGGDGDLNTPVYNYDLIPAYPSLGSTSPQLEIWATPFAHFDGTIYQPRGAWTR
                     LQASGNYNGPLRIVSGALKTQGSGLLRLTGPAIQTIVYVTALVE"
     gene            complement(2736..>4165)
                     /locus_tag="VLE22_16770"
     CDS             complement(2736..>4165)
                     /locus_tag="VLE22_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16109.1"
                     /translation="SARATAAVAHSAGMGSLILINRQNDCIPMEGGSQLSCGVDLLVQ
                     ANDNQGRYALQADGGILMASTSNGADKNGRFAGENQGGGTVNAPFTYIREEGWYTNGG
                     SSNWFQTPVNRKPDGDPFRDPMRGKYQPPPPPPDKVVEPAMFPQGVVGGTITGTDDPA
                     NPLVLQPGAYYASALDPKTNETYATGQPVQITGAVYFSKGAEDFGEYVFFGGVSAIQA
                     GQGSTNLRFDPGRYVFAGRMPQANGDPLPVFDITTNLTMTDGTDAFQANNGPQADPGE
                     IFIFTDPNYVGWDWTTQTAVPLRVPAKLTTDLGGKLGRIVDQLEFGISGFQAGNNTDV
                     VVNLHGINRQNSIVPETLRKFEAPILLWQDQNNSVLRYYDDPADPLTFNKGCVIGDDP
                     TLCANTRLASNKSPELFFKASPGSHLYGVIYQPRGAWTTLGGGSGYSGPLQIVAGGFK
                     IQGNAVLHLAGLNDPMLRNIVALVE"
BASE COUNT          800 a         1275 c         1257 g          833 t
ORIGIN      
        1 gggaaccggg aatatgcgtg cgagctggtg ttgagggaaa tggagcggtt gcccaatgtc
       61 ccggacccag cgagcgccta tacggacctc tcgtgcagtg acctgccggc cagcgtccag
      121 gtggacgcct ccagcggact cgatgccgac gggaagttgt tctcgcgcgt gcaggtgagg
      181 tatgagaccg tccaactgat accgatcccc gggctgctca ccggcaagac cacaataact
      241 cggaccgccg agtcgcggat gtacggaaaa acgaggccgt agctatggag ccgagcaaga
      301 aacaacgagg aagcaccctg ctggagttca cgcttgtcgg gattccggtg atcttcatgc
      361 tgatctcgat cttcgaaatc tcccgtggca tgtggctcta tcacacgctt gccagcgcca
      421 tcaaagatgg cacgcgcttt gccatcgtcc acggcaacaa ctgcaacata taccccagca
      481 attgtgtcgt cacgatccgc gatatctctg agcggattcg ccgcaatgcc gtcggcttcg
      541 tgccggatga cctgcagaac ctggagttca tttccgctac ccgtggcagg ttcacctgcg
      601 caacgctcgc ggcctgtctc gataccggaa atccaggtga cctgtcctgg ccgggcggtc
      661 cggtgggaag tgcgcccgaa gatttccctg atggaagaca gttcgcgtgg gtcgagattc
      721 gagcgcagta tccgttccgc tccgcgattg caatgttctg gcccggtgcg ggccgaggtc
      781 agaatttcgg ggagtttatg ctccccgcca gttcgaggga tcgaatccag tactgaggag
      841 gttgagtatg gcgaaaaacc ttggaaaacg ccgaggccag gtgctcatca tggtcaccct
      901 ggctcttctg atgctgtgcg gcatgctcgg gctggtggtg gacctcggct ggtcctattt
      961 cacgaagaag tctgcccagg ccgccgcgga cgctggggcc ctcgccgcgg tcaaagcagc
     1021 catggatgac gcagtggaca tctcctcgta cacctgcggt ggcggtggag ctgagtgtct
     1081 gagtgttccg accccatgcc caaacctccc cggaaacttg cagagcgcgt gtatgtacgc
     1141 ccagcagcac ggtttcacca ccggtggacg ccagttggtt acggtgcagg cctgggaccg
     1201 cacccccaac ccgactgtgg tcgagaactg cggccagggt ggcgcctcgg tcgcgcatcc
     1261 gcccacggca gacccgccgt gcgttgatac gttctattgg gtcacagtca gagtcaccga
     1321 gcggatcccc caattgtttt cgtcggtctt tggaaacaca gaaggcatcg tcagcgcccg
     1381 cgcgaccgcc gctgtcgcaa agtcggtcac gccggcgtcg ctcattctga tcaaccgtga
     1441 gaacgatccg tggaccgacg atagcggtca ggtctatacc ggcaacaacc tctatgtcgg
     1501 tggcacgccg caagtgatag taccaggtgg aatcatcatg gcgtcggggg cgggtggtcc
     1561 ttccaaacag gacgcgtggg ccggttacat cgccggcagt ggcacggtgg actcgcaggg
     1621 cggcacctac attcgtgaca acggtgacta ctacatctcg ggtggcgggt cgtggaccat
     1681 cacaccggag aaccagcctg acaacgaaat cttctgggat ccatacgaag gcattgccca
     1741 gccgccggtc gcgtcgaacc ccctcactcc gatccctgtt ccgaatggca atctcggctc
     1801 cctcaagaac accgtctgca agagcggctg cccatcaggc aactatttcg cgaccggtat
     1861 cccaaaaggc tgcaacaact gcgcggaggt cgcgaccgga gagcagattg tcatcaacac
     1921 ggacattgag tttaatggcg ggggtttcgg cgatttcaac ttcttcggcg ggctgaacat
     1981 cggccaggcg aaggtaacgt ttgggcccgg ccgttacgtg atggccggca ccctgaacga
     2041 gaacatcccg ctgttcaaca cggacaacaa ggcgaagatt atcggtggta acgggggcga
     2101 tgcgggccgg cttttcatcc tcaccgactc gcagtacccg ggcctcgaga ctcaggtggc
     2161 gagcatctcc tatacccggg agtggccggg ccccaacaac tccctgacat tttccaagag
     2221 cagtatcaag tccgggaata acgagggatc ctcgacggat ctgtacagcc tgtccaagga
     2281 catcggcaac acggtgcccg agttggcgcc gtaccagggt ttcctgcttt ggcaggaccg
     2341 caggaactct tacgtccact acaaggacgc ctacaagtat gtagatgacg gtggcgatgg
     2401 tgacctgaac acgcccgtct acaactacga cttgatcccc gcctacccct cactcggcag
     2461 cacgtccccc cagctcgaga tctgggcgac cccgtttgca cacttcgacg gtactatcta
     2521 ccagccgcgc ggcgcctgga cgagactgca agcctcgggg aactataacg ggccgcttcg
     2581 tatcgtgagt ggtgcgctca agactcaggg ctctggcctc ctccggttga ctggcccggc
     2641 cattcagacc atcgtctacg tgaccgcttt ggtggaatag aaactgcaga aatcaggcag
     2701 gggcacgccg ggcggcgtgc cccgaaggtg ccgcttcact ccactaacgc aacgatattc
     2761 cgcagcatcg gatcgttcag ccctgcgagg tgtagaacgg cgttcccctg aattttgaat
     2821 ccgcccgcta caatctggag cggccctgag tacccgctgc cccctccgag ggtcgtccac
     2881 gctccgcggg gttgatagat tactccgtaa agatggctcc cgggtgacgc tttgaagaac
     2941 agctccggtg acttgttgct ggccaggcga gtgttcgcgc aaagggtggg gtcgtccccg
     3001 atgacgcagc ctttgttgaa tgtcagcgga tcggccggat cgtcgtagta ccgcagcacc
     3061 gagttgttct ggtcctgcca gagcagaatc ggcgcctcga acttccgcag cgtctccggg
     3121 acaatcgaat tttgacggtt gatcccgtgc aggttgacca caacatcggt gttgttgccg
     3181 gcctggaaac ccgagatacc gaactcgagt tggtccacaa tccgcccgag tttcccgccc
     3241 aagtccgtgg tcagcttcgc cggaaccctg agaggcacgg ccgtttgcgt agtccagtcc
     3301 catccaacgt agttcgggtc ggtgaagatg aagatctcgc ccgggtcggc ctgtggtccg
     3361 ttatttgcct gaaacgcatc cgtgccatcg gtcatcgtca ggttcgtcgt gatgtcgaag
     3421 accgggagag gatcgccgtt cgcctgcggc atgcggccgg cgaagacgta acggcccggg
     3481 tcgaagcgga ggtttgtcga accctggcct gcctgtatgg cgctgacgcc tccgaaaaac
     3541 acatactcgc caaagtcctc tgcgcctttc gaaaaataca cagctccggt gatctgcaca
     3601 ggctgcccgg tggcatatgt ctcgtttgtc ttcgggtcca aagcggacgc atagtaggct
     3661 cccggttgta gcaccagcgg gttggccggg tcgtctgtgc ccgtgatggt tcccccaacc
     3721 actccctgcg ggaacatcgc gggttcgacc accttgtccg gtggcggagg cggtggctgg
     3781 tacttgcctc gcatcgggtc gcgaaacgga tcgccgtcgg gtttacgatt gaccggtgtc
     3841 tgaaaccagt tcgaactgcc gccgtttgtg taccagcctt cttcacggat ataggtgaat
     3901 ggcgcgttca cggtgccgcc gccctggttt tcgccggcga agcgtccgtt cttgtcagct
     3961 ccgttgctgg tggaagccat gagtatcccg ccgtccgctt gaagcgcgta ccggccctgg
     4021 ttgtcgtttg cctgcaccag cagatccaca ccgcacgaaa gctgactgcc accctccatc
     4081 ggaatgcagt cgttctggcg gtttatcagg atgagtgaac ccatcccggc cgagtgtgcg
     4141 accgccgccg tcgcccgggc gctcg
//
LOCUS       DATIFQ010000350         3326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1074653, whole genome shotgun sequence.
ACCESSION   DATIFQ010000350 DATIFQ010000000
VERSION     DATIFQ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074653"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VLE22_16775"
     CDS             complement(<1..223)
                     /locus_tag="VLE22_16775"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HSB16110.1"
                     /translation="MTIAPGVVLPEAQIAEVCRRYHVKELSLFGSAARGELRPDSDVD
                     LLVDFLPDARVGLLDLSALMRELADLLGRR"
     gene            302..2074
                     /locus_tag="VLE22_16780"
     CDS             302..2074
                     /locus_tag="VLE22_16780"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HSB16111.1"
                     /translation="MAVARCLAGLLVASALFGQTEFFPLEKVRAGMRGTGKTVFSGNR
                     VEEFQVEILGVLENVGPKQSLILARLAGGPLEKTGVLQGMSGSPVYLEGRLLGAVSMA
                     FPFSKEPIAAIRPIGEMLLSARGGKARTVRARVSLLDEDLTRPFTPPEEVRAGGARLI
                     DIATPVSFGGLTRNTVEHFAPQLRALGLEPAQGMSGGGKARASDGATPPFEPGSMITV
                     QLMSGDMSIGADGTVTYVEGKNVYAFGHRFLSIGNTELPFARSEVLTLLPSLASSFKI
                     SAVREWMGTILDDRSTAVSGLLGRRTATVPLSIEVTRRGEHAGGARSSYRMEMVNDRY
                     LAPFLVQMAVYSAIDATERTVGSSTLALNGVFEFRDGTTPIRLNNSFSGEFSVPQQVS
                     LATSIPLAYALQSGFDSLQLKNVKLEIESFDEKKQAQIEQVWTSRQEVRAGDAVELTV
                     VLASPNGAEVKRKVTYEVPVGAPAGPLYFTVADGTTTNLTEIRQFVGAQPRSPSQLVS
                     LVNSLRGNTKAYVRIWRPDANYDVQGHDFPSPPPSLEQVLVQTQAAVGGGFPGRSSKV
                     AELEIDAGGVVVTGSKTVQVDIKG"
     gene            2071..>3326
                     /locus_tag="VLE22_16785"
     CDS             2071..>3326
                     /locus_tag="VLE22_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16112.1"
                     /translation="MRYGLPLILLALMAARGNAAGTATWEMGSYQDFLRGQFTGVSLS
                     RDGRLMLAPKTETLFSSDQPVIWSVAQAPDGTLYAATGHRGRVFKVDKSGKSEVVWTA
                     DQPEVFAVALDAKGTLYAASSPKGKIYRIEGGKAVEFFSPGSVYIWSLVFARDGTAFV
                     GTGDEGKVFRVDRSGKGEIYYETGQTHVTTLALDTQGRLLAGTEPNGILYRIEAKGKA
                     FVLYDADLPEIRAITTASDGVIYAAAQGGASTRRGIAATQAAPGGMGKPVVSATATTI
                     TVTDEAQGGVEIKPKPEAAKQAPAVTPQVTTQFSPIVDVSGIEKSALYRIWPDNTVET
                     LWSSKDENVYDVLPSGGQIVFSTDGKGRIYRLGADRKVTLLVQTNESEATRLVQGAGG
                     LLAATGNLGKIFRLSDATEASGVYEAP"
BASE COUNT          613 a          974 c         1163 g          576 t
ORIGIN      
        1 cacgacgacc caggagatcc gccaactcgc gcatcagcgc cgacagatca agcagcccca
       61 cgcgggcgtc aggcaggaaa tctaccagca gatccacgtc gctgtccggg cgcagctcgc
      121 cgcgggccgc cgagccgaag agcgaaagct cttttacgtg gtatcggcgg caaacttcgg
      181 caatttgagc ttccggaagt accacacccg gcgcgatcgt catggaacga gctcctgtca
      241 ctcattatga accagtcccc ggccgggtga ggccgtgtcc cgccggcggt agaatgagag
      301 cgtggcggta gcgcggtgtc tcgcggggct cttggtggcg tctgcgctgt tcgggcagac
      361 cgagtttttc ccattggaga aggtgcgcgc ggggatgcgc ggcacgggaa agaccgtctt
      421 ctcggggaac cgggttgagg aatttcaggt tgagattctc ggcgttctgg agaatgtcgg
      481 tccgaagcag tccctgatcc tcgcgcgcct ggccggcggg ccgctcgaga agacgggcgt
      541 gcttcagggg atgagcggca gtccggtgta tctggaaggg cggctgctgg gcgccgtctc
      601 gatggccttt ccgttctcga aggagccgat cgccgccata cgtccgatcg gcgagatgtt
      661 gctttcggcc cgaggcggca aagcccgcac ggtgcgggcg cgtgtttcgc tgctcgacga
      721 agacctcact cgtccgttca ccccgcccga agaggttcgc gcgggcggag cgagactgat
      781 cgacatcgca acgccggtgt cgttcggcgg cctgacgcga aacaccgtcg agcactttgc
      841 gccgcagttg cgcgcgctgg gactcgagcc ggcgcaggga atgtccggcg ggggaaaggc
      901 gcgggcgagc gatggcgcga cgccgccctt cgaaccgggc tccatgatca cggtgcagtt
      961 gatgagcggc gacatgagca tcggtgcgga cggcacggtg acctatgtgg agggaaagaa
     1021 cgtgtacgcc ttcgggcacc gttttctttc catcgggaac actgagctac cgtttgcgcg
     1081 ctccgaagtg cttacgttgc tgccgagcct ggcctcttca ttcaaaatct cggccgtgcg
     1141 ggagtggatg ggtacgatcc tcgacgatcg cagcacggcg gtcagcggat tgctgggaag
     1201 acgcaccgcg acggtaccgc tttcgatcga agtgacgcgg cggggcgagc atgccggcgg
     1261 cgcccggtcg agctatcgga tggagatggt gaacgaccgg tacctggcgc cgtttctggt
     1321 gcagatggcg gtctactcgg cgattgacgc caccgagcgg acggtgggca gttcgacgct
     1381 ggcattgaac ggcgtcttcg aatttcgcga cgggacgacg ccgatccggc tgaacaactc
     1441 attctcgggc gagttcagcg tgccgcaaca agtgtctctt gcgacatcga ttccactcgc
     1501 ctatgcgctc caaagcggct tcgactcgct tcagctcaag aacgtgaagc tcgagatcga
     1561 atcgttcgat gagaagaagc aggcgcagat cgaacaggtg tggacctccc gccaggaagt
     1621 gcgcgcgggc gacgcggtcg aattaacggt ggtcctggcg tcgccgaacg gcgcggaggt
     1681 gaagcggaaa gtgacatacg aggttccggt cggggccccc gctgggccgc tttacttcac
     1741 cgtggccgat gggacgacga cgaacctcac cgagatccgc caattcgtgg gcgcgcagcc
     1801 ccgatcgccg tcgcaactgg tgtctttggt caactcgctc cgcggcaaca caaaggcata
     1861 tgtgcgcatc tggcgcccgg atgccaacta cgacgtgcag ggacacgact ttccgtcgcc
     1921 gccgccgtcg ctcgagcagg tgctggttca gacgcaggcg gcagtcggag gaggctttcc
     1981 cggacgaagc tcgaaggtcg cggaacttga gattgatgcc ggtggagtgg tcgtgacagg
     2041 atcgaaaacc gttcaggtgg acatcaaagg atgagatacg gcctgccgtt gatcttgctt
     2101 gctctcatgg ccgcgcgcgg aaatgctgcg ggcacggcca cgtgggagat gggcagctac
     2161 caggatttcc tgcgcggtca gttcaccggc gtttcgttga gccgggatgg acggcttatg
     2221 ctggccccga aaacggagac gctgttctcc tcagaccagc ctgtgatctg gagcgtggcg
     2281 caggctccgg acggcacgct gtacgcggct acgggccatc gcggccgggt tttcaaggtc
     2341 gacaagtccg gcaagagcga ggtggtctgg acggccgacc agcccgaagt gtttgccgtc
     2401 gcgctcgacg cgaagggtac gttgtatgcc gcgtcgtcgc cgaaggggaa gatctatcgc
     2461 atcgagggcg ggaaggctgt cgagttcttt tcgccgggct cggtgtacat ctggtcgctc
     2521 gtgtttgcga gggacgggac ggcgttcgtg ggaacgggcg acgaaggcaa ggtctttcgc
     2581 gtcgatcgct ccggcaaggg cgagatctac tacgagaccg gtcagacgca cgtcacgacg
     2641 ctggcactcg acacgcaggg gcggctgctc gccggcacgg aaccgaacgg catcctgtac
     2701 cgcatcgagg cgaagggcaa ggcgttcgtt ctgtacgacg cggaccttcc cgaaatccgg
     2761 gccatcacga ccgcttccga cggggtgatc tacgctgccg cgcagggtgg tgcgagcact
     2821 cgcaggggca tcgcggcgac gcaggcggcg ccggggggaa tgggcaagcc ggtcgtgagc
     2881 gccacggcga cgaccatcac cgtcaccgat gaagcgcagg gtggggtcga gattaagccc
     2941 aagccggaag cagccaaaca ggcgcctgcc gtgaccccac aagtgacgac gcagttcagc
     3001 ccgattgtgg acgtgagcgg catcgagaag tcggcgcttt acaggatctg gcccgacaat
     3061 acggtcgaga cgctctggag ctcgaaggac gaaaacgtgt acgatgttct gccctcggga
     3121 gggcagatcg tcttttccac ggacgggaaa ggccgcatct accggcttgg cgccgatcgc
     3181 aaggtgaccc tgctggtcca gacgaacgag agcgaagcga cccgcctggt gcagggcgct
     3241 ggtggattgc tggcggcaac gggcaacctc ggcaagatct tccggttgag cgacgctacc
     3301 gaagcaagtg gcgtgtatga ggcgcc
//
LOCUS       DATIFQ010000351         4624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_500344, whole genome shotgun sequence.
ACCESSION   DATIFQ010000351 DATIFQ010000000
VERSION     DATIFQ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500344"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VLE22_16790"
     CDS             complement(<1..267)
                     /locus_tag="VLE22_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16113.1"
                     /translation="MNRLGRRAFLAACSASALPLRASPLARIPRENLKITDIRVTLLS
                     HELPREKQWVTASFVVWKADAILVEVLTDKGIIGIGEPSPYGGPE"
     gene            complement(264..1019)
                     /locus_tag="VLE22_16795"
     CDS             complement(264..1019)
                     /locus_tag="VLE22_16795"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16114.1"
                     /translation="MKTRYFLIVAVLVFAVSIVVAVSGGSQQVAADPIFDPIIEGFQK
                     STVASVADAVDQVVGERGFLSHDMRPMIPGKFVGRARTALVRPAPPEKATPALAVKHS
                     VEMIEGSKPGDVGVIVMEDGLDVAAIGGLMATTAKAREMAGFLIDGGVRDVGEIRALD
                     LPVYARSVTPATAVGRYASVSQGEPVTCGGVTIKPGDIIVAGEDGVVRVPAGKAAEVL
                     AKSQEIDERESKMVPFIKKFKSLTKAIQVFNRI"
     gene            complement(1016..1870)
                     /locus_tag="VLE22_16800"
     CDS             complement(1016..1870)
                     /locus_tag="VLE22_16800"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16115.1"
                     /translation="MGIRMMAAVAITAGFCAGQVTPDPKLRASRDTYHPVMEVLKQQR
                     TELTVSQIDELRKLPIEAVWAVLQRLGYTNCYHAGLQSTRPTEKLAGRALTIRYLPRR
                     PDLDEAMNTLANEGDWPRAYNVRAAEEAKPGDVLVVDLGGTVPDGVFFGDISALGAQV
                     AGARGAVLWGSTRDLSELRLMEGFPVLAVGFDPKGAVQVGVDWNVPVRVGSVTVIPGD
                     AVLADEEAVLFFPPQLADKVIEQVKTYVEREDYERDLARQKKHRFRNVYPMSPELQKQ
                     FEETRKKK"
     gene            complement(1872..3125)
                     /locus_tag="VLE22_16805"
     CDS             complement(1872..3125)
                     /locus_tag="VLE22_16805"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB16116.1"
                     /translation="MPKISRRAVLAGAAGALSLEGLSASLQNAVSPYRRPKLKITDVR
                     TAMVRAHGNQLHVKIYTDQGLVGHGEATDAATGGQPLVASFRRMLIGEDPLNVEALFE
                     RIRTAGIFAGAQAGQYVAALSGVEIALWDLAGKAVGLPVYQLLGGKVRDRIRLYCDTQ
                     APDPEETRAKERFRRIEELGFTAIKIDLDDGRDPMRWDPVNWTASNAEIDTMVRKASV
                     AREALAKRTDLAADMHGRYDMTTGKRVAIELEPLRLLWLEEPVPAENVDAMRDIREST
                     KTPICCGENLFLRHGFREVLEKRAADILMPDIQKCGGLLESRKIADMAHTYYVPVAPH
                     CVVSPVGAMASCHVCAAIPNFLVLEWHWIQRLEIWRQFVKEGDIIEKGFIPVPERPGI
                     GVEMNDEVAKQHRAPGTGWFEPLGE"
     gene            complement(3158..>4624)
                     /locus_tag="VLE22_16810"
     CDS             complement(3158..>4624)
                     /locus_tag="VLE22_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16117.1"
                     /translation="IERDGGIMLGKLKSPTEADKSQVDDPWYPADVKEAAMEGLAELF
                     TTTKDNWLFPHTRYSQGHTMDFVLRPGERMIRYYKPEKPGLYYLPYKYDGRQWSEFPR
                     EVAQYKIRTADGPHSQRDGRLWGTGVIEYKPPVIPTASPAVIEVSSPYVIIDAEFTMR
                     AAMARGEELSIETSTDGGRAWFKSGEISGAHNGPWNAEPAVKTTSEHGRISAVSGTYG
                     YLVRLTRTGGVKLQDFQINTRFQLNPRTLPELQPGRNEMVYSPGAPQRRRVLPIRTDQ
                     VERVALKTANYRLVLEGGQSYAVARDAGPAEFLFELSSPDGVTLSGFDAGGRFLDIRD
                     NLAPDKLTAETRKTALRSTGTSGKRAASLSWSTAPDGEFRELWSYDEKLTWRDGVPID
                     RLLRWPEVDRQVRTLPPGTRKVYVRYNVEGVAVDDFRLATVTPARPGSQLTITHVWRE
                     GGQQRTHAEKIVNPRTTQSYAIEAGPGAEPEALILECR"
BASE COUNT          780 a         1481 c         1446 g          917 t
ORIGIN      
        1 ctccggaccg ccataggggc tcggctcgcc gatgccgatg atgcccttgt cggtgagaac
       61 ctcgacgagg attgcgtcgg ccttccagac gacgaagctg gcggtcaccc attgcttctc
      121 acgcgggagt tcatgggaga gcaatgtcac gcggatgtcg gtgatcttga gattctcacg
      181 agggatgcgc gcaagcgggc tcgcgcgcag aggcagcgca ctggccgagc aagctgccag
      241 gaacgccctg cggccaagcc ggttcatatc cggttgaaca cttggatcgc cttcgtcagc
      301 gacttgaact tcttgatgaa ggggaccatc tttgattcgc gctcatcgat ctcttgcgat
      361 ttggccagga cctcggccgc cttccccgcg ggaacccgca ccacgccatc ctcgcccgcg
      421 acgatgatgt cgcccggctt gatggtcacg ccgccgcaag tcaccggctc gccctgggag
      481 acgctcgcgt agcggccgac cgccgttgct ggcgtcacgc tgcgggcgta cacggggaga
      541 tccaaagcgc ggatctcgcc gacgtcgcgc acgccgccat cgataaggaa accggccatt
      601 tcgcgcgcct tcgccgttgt ggccatcagg cccccgatgg ccgctacatc gaggccatcc
      661 tccatgacga tgacgccgac gtcgccgggc ttcgaacctt cgatcatctc gaccgagtgc
      721 ttcaccgcaa gggcgggggt ggccttctct gggggcgccg ggcggaccag tgcggtccgc
      781 gcgcgtccga cgaacttgcc cgggatcatc gggcgcatgt catgcgaaag aaagccgcgc
      841 tcgccaacga cttgatcgac cgcatcggcc accgaggcaa ccgtcgattt ttgaaatccc
      901 tcgatgatcg gatcgaagat cggatcggcg gccacctgct gggaacctcc gctcaccgct
      961 accacgatcg agaccgcgaa cacgagaacc gccacaatca ggaaatatct ggttttcatt
     1021 tcttcttcct cgtctcttca aactgtttct ggagctccgg gctcatcgga tagacgttcc
     1081 ggaaccggtg cttcttctgt cgggcaagat cgcgctcata gtcctcccgc tcaacgtacg
     1141 tcttgacctg ctcgatcacc ttgtccgcaa gttgcggcgg gaagaacagc accgcctctt
     1201 catcggccag taccgcgtct cccggtatca ccgtcaccga ccccacccgc accggaacat
     1261 tccaatcaac cccaacctgc acggcgccct tggggtcgaa gcccaccgcc aggacaggga
     1321 agccttccat cagccggagc tcggagaggt cgcgcgtcga tccccagagc accgcaccgc
     1381 gcgcccccgc cacctgcgcg ccgagcgccg agatatcacc gaagaagacg ccgtcgggaa
     1441 cagtgccgcc gaggtcgacg acaaggacat cccccggctt ggcctcttcc gcggcgcgga
     1501 cattgtatgc gcgaggccag tcgccttcat tggccaacgt gttcattgcc tcgtcgagat
     1561 ccggccgccg cggcaggtaa cggatcgtta gtgcgcgtcc tgcgagcttc tctgtcggac
     1621 gcgtggattg cagcccggcg tggtagcagt tggtgtagcc cagccgctga agaaccgccc
     1681 acacggcctc gatgggcagc ttcctgagtt cgtcgatctg cgagactgtg agttctgtgc
     1741 gctgctgctt gagcacctcc atcaccggat ggtaggtgtc tcggctcgca cgcagctttg
     1801 ggtccggcgt cacctggccc gcgcaaaaac cggctgtgat cgcgactgcc gccatcattc
     1861 ggattcccat ctcattctcc taaaggctcg aaccagccag tgccgggcgc gcggtgctgc
     1921 ttcgccactt cgtcgttcat ctccacgccg attcccggcc gttcgggtac gggaatgaat
     1981 cctttttcga tgatgtcgcc ttccttgaca aactgcctcc atatttcgag ccgctggatc
     2041 cagtgccact cgagcaccag gaaattcggg atcgcggcgc agacgtggca agacgccatc
     2101 gctcctaccg gcgagactac acagtgtggc gcgaccggaa cgtagtaagt atgcgccatg
     2161 tcggcgatct tgcgcgactc gagcagtccc ccgcactttt ggatgtcggg catcaggatg
     2221 tccgcggctc tcttttcaag cacctcgcgg aacccgtgcc tgaggaacag gttctcgccg
     2281 cagcagatgg gcgtctttgt cgattcgcgg atgtcgcgca tcgcgtcgac attctcggcc
     2341 ggcaccggtt cttcaagcca gaggagacgg agcggctcga gttcgatcgc cacccgcttg
     2401 ccggtggtca tgtcgtagcg gccatgcatg tcggccgcaa ggtccgtccg cttggccagg
     2461 gcctcgcgag cgacggaagc cttacggacc atggtgtcga tttccgcatt gctcgcagtc
     2521 cagttgaccg ggtcccaacg catcgggtcc cggccatcgt cgagatcgat cttgatcgcc
     2581 gtgaatccga gttcctcgat ccggcggaag cgctctttcg cccgcgtttc ttcgggatcc
     2641 ggcgcctggg tgtcgcaata gaggcggatg cggtcgcgca ccttgccgcc gagcaattga
     2701 tagaccggca agccgacggc cttgccggcg aggtcccaca acgcaatctc gacgcccgaa
     2761 agggcggcga catactgacc ggcctgcgct ccggcgaaaa ttccggccgt gcgaatcctt
     2821 tcgaaaagtg cttccacgtt gagcggatct tctccgatga gcattcggcg gaacgaggcg
     2881 acaagcggct gaccgcccgt cgcggcgtcg gtggcctcac catgaccgac gagtccctgg
     2941 tcggtgtaga tcttgacgtg aagctggttt ccgtgagcgc ggaccatcgc tgtgcggacg
     3001 tcggtgattt tgagttttgg tctccggtat ggagagaccg cgttctgaag tgaggccgat
     3061 agtccttcga gactgagcgc tcccgccgcg cctgcgagga cggccctcct gctgatcttc
     3121 ggcatcgagt ccgattatat gccgcgcgag ggcggaatca acggcactcc aggatgagcg
     3181 cttcgggctc cgcgccgggg cccgcttcta tggcgtacga ctgagtcgtg cgcggattga
     3241 cgatcttctc tgcgtgggtc cgctgttggc caccttcgcg ccagacgtgc gtgatcgtga
     3301 gctggctgcc ggggcgggct ggagtaacgg tagcgaggcg aaaatcgtcg acggcaacgc
     3361 cctcgacgtt ataacggacg tagacttttc gcgtgcccgg cggcagcgtg cgcacttgac
     3421 gatccacctc gggccagcgc agcaggcggt cgatcgggac gccgtcgcgc caggtgagtt
     3481 tttcgtcgta gctccacagc tcgcggaact ctccgtccgg tgcggtggac cacgagagtg
     3541 atgccgcgcg cttgccggac gtgcccgtgg accgtagggc cgtcttgcgc gtttcggcgg
     3601 tcagcttgtc cggggcgagg ttgtcgcgga tgtccaggaa tcgtccgccg gcatcgaaac
     3661 cggaaagagt cactccgtca ggggaggaca actcgaagag aaactccgcg ggcccggcgt
     3721 ctcttgcgac agcatagctc tgtcctcctt cgagcacgag acggtagttg gcggtcttta
     3781 aagcgacgcg ctcgacctgg tctgtccgga tcggcaggac cctgcgccgt tgcggtgctc
     3841 cgggactgta gaccatctca ttgcggccgg gctgaagctc ggggagggtg cgcgggttca
     3901 gttgaaatcg cgtgttgatc tggaagtcct ggagtttgac gccgccggtg cgagtcaggc
     3961 gcacgagata tccgtaggtt ccgctgacgg cactgatccg gccatgttcg gaggttgtct
     4021 tgacggccgg ttcggcgttc cacgggccgt tgtgcgcgcc agagatttca ccagacttga
     4081 accatgcccg cccgccgtcg gtcgaagttt caatggagag ttcctctccg cgcgccatgg
     4141 cggcgcgcat cgtgaactcc gcatcgatga tgacgtacgg gctcgacact tcgatgaccg
     4201 ccgggctcgc cgtggggatg accggcggct tgtactcgat cacacccgtt ccccacagcc
     4261 ggccgtcgcg ctggctgtga ggcccgtcgg cggtgcggat cttgtactgc gccacctcgc
     4321 gcgggaactc ggaccactgg cggccgtcgt atttgtaagg aaggtagtaa aggcctggct
     4381 tctcgggctt gtaatagcgg atcatccgct cgcctgggcg gagaacgaaa tccatcgtgt
     4441 gcccctgtga gtaccgcgtg tgcgggaaca accaattgtc cttagttgtc gtaaaaagtt
     4501 cagcgagacc ttccatggcg gcttctttga cgtctgccgg gtaccaggga tcgtctactt
     4561 gcgacttgtc ggcctcggtg ggtgacttca gcttgccaag catgattccg ccgtcgcgct
     4621 cgat
//
LOCUS       DATIFQ010000352        20486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_115400, whole genome shotgun sequence.
ACCESSION   DATIFQ010000352 DATIFQ010000000
VERSION     DATIFQ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 20486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20486
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115400"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VLE22_16815"
     CDS             <1..463
                     /locus_tag="VLE22_16815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB16118.1"
                     /translation="ESLGYPPELLVPLPAESVEAFSGVSNVSLFAEIPRGSVVLDLGC
                     GAGLDSVVAARRTGPAGRVIGIEFSEEMSSRAQRGAAAAGFRNWLVCQADAERLPILD
                     NAVDVALANGIFNLNPARKAILRELARVLRPGGAVYGAELILSEPCRRGGR"
     gene            complement(734..1774)
                     /locus_tag="VLE22_16820"
     CDS             complement(734..1774)
                     /locus_tag="VLE22_16820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB16119.1"
                     /translation="MNISELALSLIAGTILLSRAAWPSGTEELKTADDGLLEALNRHD
                     AEEILGRFHKEFVYLSVDAEAPADWAARSSEQRREVVRALFSPFKSWSVQPEEGTLYR
                     VIGDTGIVCGVERVERQRENHAPEMVRQRFMRTWQKANGRWLLIAAHRSDFAGEALAR
                     FPLPESEAEKKIIEVAEAAPRFANVTLSDARVLRMLAEFSGAKTVLEFGTSTGYSGLW
                     FANGLRKTGGRLMTFELDKDRAAIARDNFKKAGVETVVTLVEGDAHKELAKVKGPVDL
                     VFIDAEKEGYRQYLEQVLPLVRPGGMIVAHNMRYPTARPEFVEAVTTNPKLDTVFIHM
                     HDRGMSLSLKKR"
     gene            complement(1942..3585)
                     /locus_tag="VLE22_16825"
     CDS             complement(1942..3585)
                     /locus_tag="VLE22_16825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB16120.1"
                     /translation="MIDPHPNGSRTRILLSSVFGPYARDDEFGSRAINPMELYHNQVT
                     RAQGSFSLRRFHRSWGIMMIQQNISAPCTVLDFPTREAFACELSTNQYDVVGISSIIV
                     NVGKAREMCRMVRKLSPHSTIVVGGHVTAIPGVEHILDADHIVKGDGIAWMRRYLGED
                     VDAPILHPALSSGFDLRVMGIKVPEKTDSSATVIASVGCPMGCNFCTTSAFFGGKGKV
                     LNFYSTGEELFRVMEEAETSRNVKSFFIMDENFLLQKERAMDLLARMKQARKSWALNI
                     FSSANAIRKYSCEKLVELGVSSIWLGLESPRSNYSKLDGADTLRMARELRGHGIMLLG
                     STILGLEHHTPENITEEIEHAIAHETDLHQFMLYTPVPGTPLYRDMAERGKLLDVDLA
                     DIHGQFGFNFQHAAISREESKRLLDWAFLRDFERNGPSLFRICRTTFEGWKRHRNHPD
                     LRVRERFEREASSLRRVYAGILWAMEHRLKRTNHSVTLQIRALRKDLEREFGVLTAAT
                     ARLLGPVLWWTSIREDKRLARGHSYEPPTIIERSNWIEA"
     gene            complement(3582..4778)
                     /locus_tag="VLE22_16830"
     CDS             complement(3582..4778)
                     /locus_tag="VLE22_16830"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSB16121.1"
                     /translation="MKNFSELSLCSRLQSNLAKQGFVQPTAVQARAIPPALAGQDVVA
                     TAQTGTGKTLAFLLPLLQSLMGGSCKSGINALILSPTRELAMQTNESFSRITAGTGIR
                     SAVAVGGMSEAGQLRAIRQGAQVLIATPGRLCDFLDRRLVSLSGVHFFVLDEADRMLD
                     MGFLPALHAILKVVPASRQTLLFSATIEQSVAHLIQAYVRNPVRVAIGPSTKPADQVN
                     LHVYEVESDRKLGLLTRLIGQEPGSFLVFARTKHGTDRLAKKLSHTGVKTASIHGGRT
                     QSQRNQALNGFKDGQYRVLVATDVAARGIHVDGIAHVVNYDLPHVPEDFVHRVGRTGR
                     AGARGTASTFSTRNERNEIRRIERVLNVRLTPRSVSADLPRDSRFSRPLEQKRKGTFS
                     VEPSPR"
     gene            complement(4906..5907)
                     /locus_tag="VLE22_16835"
     CDS             complement(4906..5907)
                     /locus_tag="VLE22_16835"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSB16122.1"
                     /translation="MDAFSEILNGVKLKGALFFNAELSAPWSVASPRSDCLAPHLVPG
                     AEHLIIYHFVIEGSAYAQLEGGNLLSLNAGDIVIFPHGDPHRMASSAREQSIEISDIL
                     PLIRRRSLGLMRTGGGGEVSRFICGYMACEPHLFRPILSGLPRMMKVNIRADGSGRWL
                     ENCIRHLVDEAGAGQPGSEAMLAKLSEALFVDTLRRYVAALPAEQTGWLAGTRDPVVG
                     KSLAILHSRVEQPWTIAQLAKEVGLSRSALVERFSRYLSEPPMAYLTRWRLQLAARSL
                     MSTPRSVAQIAAEVGYESEAAFNRAFKRAFGTPPARFRSEHKPKTGGITSLAASSGG"
     gene            6094..6870
                     /locus_tag="VLE22_16840"
     CDS             6094..6870
                     /locus_tag="VLE22_16840"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HSB16123.1"
                     /translation="MKKALSVTYGALAYVMFLGAYLYLIAFTVNAVPNSISGPTSLPP
                     LLAAAIDLGLITIFGLQHSIMARPGFKRLWTRIVPPHLERSTYVVIGSVMVVLIVVCW
                     QPIGGEIWNVTGAGMVALYAIAALGWLVVPVTSFLTDHFELFGLRQVFEYALGRPKSK
                     PMFKVRAFYRKVRHPMMLGWMVGFWATPYMTTGHLLFAGLMTSYILVAIHFEERDLAR
                     EHGPAYRAYQARVPKLVPFSKPAGAEQGAFEGMVGEPQIQ"
     gene            complement(6946..10020)
                     /locus_tag="VLE22_16845"
     CDS             complement(6946..10020)
                     /locus_tag="VLE22_16845"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB16124.1"
                     /translation="MRLSEICVQRPVFAFMLIMFLVVMGAFSFMGLGVDLFPRTDPAT
                     VYVRVRLPGASPEEMTSQVVLPIEEAVASVSGIDEMRASVLEGTANIIVTFVLEKDIG
                     EAAEDVREKVSGAMRRLPPNVLPPVVQKADPDSDPIVTLAVSGDLSIRELTEIADKQV
                     RRALETVDGVASISIAGGQNRQVNVLLDLDKLSGYNLTAQDIERAIKAENIESPGGRI
                     VRGPTEMGVRTLGRLEKVAEFNDIIIKNVGGAPIRLRDVGYAEDSMTEKRTFAYYQDK
                     PAVTLEARRQTGTNTVQVVDGILARVERLGRELPAGVKLEVVKEQATYIKSSVKALEE
                     HLVIGSLLASLIVWLFIRNWRTVLISSIAIPTSIITTFTLMKMMDFTLNSMTLLGLTL
                     AVGIVIDDAIIVLENIYRFIEEKLMSPMEAAVKATKEIALAVMATTLSLVIIFVPVAF
                     MQGYARRYLNQFGWTMAMAIMVSMLVAFTLTPSLSSRLLRRRTGNGAAARPEDHHGVL
                     GRIYVKTLGWSLDHRWVIVSVSVLTFASTFLLSRHIGRDWMPQEDQNELSVMLEMPEG
                     ASLEATEKVTLEISRKIRKIPGVVTVVPQTSSFADRVTMAWMTILLKDVSERESIEQM
                     GQHVRAVLRDYPYTRPRVTFPNALGGHETFSPIRAQLLGPDLGKVMELAKQASIELTH
                     ADAVADIKVNLNLSNPEFQVDIDRSLASDLGVRVSDIASAVRLLMSGEDEISTFKEGS
                     EQYPVTMRLLPSQRDDPNALSRLLVPSARLGLIRLDSVAHVDRGLGPSRIDRFNRQFA
                     VGIYGNVAPGRSLGEAAAQTQEAIARVGMPAGYEMRFSGQVKILEETTANMILAIGLA
                     SIFMYMVLAAQFESLVHPFIILLTLPLSIPFALISLIGTGRSLNLFSALGVLLLLGIV
                     KKNGILQIDYMNRLRQQGQPLREAILEANRVRLRPILMTTFSIVAGLIPTAIGIGAGA
                     SQRSAIAVTIIGGQTLCLLLTLLVVPVGYSLVEDGRVWLSGRRLAAEPALGD"
     gene            10115..11341
                     /locus_tag="VLE22_16850"
     CDS             10115..11341
                     /locus_tag="VLE22_16850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB16125.1"
                     /translation="MRNRYSYVAGWVTVIFVLAVFAGCRPKPAETKKQTGPVEVHAAP
                     VVHREARRVVDSVGTLFPFDEAIISAEIEGPVEKVNVDLGDRVAQGQVMVQISDEEQR
                     YLVAQMEAQLRQSLERLGLKEENEKVKDIRLTPEVRRAQADLTDADQRLRRVRSLVQQ
                     GIGSQQDLDEAQARFQSLQAAYDAMLNQTRNLVRDVERTRAVLDLQRKKLRDTQVRAA
                     FAANVKERQVTVGQYVRPNTPLLTLVKIDPIRLRAEVPERMAPWIKVGQVAEVSVEAF
                     ENRKFRGKIWRISPTVDQSKRTFLVEALIDNRSEELKPGSYARVRVETDKVEKIRLIP
                     VSAINYVFGLNKAYVVSGGVVEARDVKLGDRYGQEVEITEGLEDGEMVATSQVARLDT
                     GVKVRLASAAGNQRVN"
     gene            11489..12526
                     /locus_tag="VLE22_16855"
     CDS             11489..12526
                     /locus_tag="VLE22_16855"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB16126.1"
                     /translation="MDNVMTRRSAIGAGLMSGMAAGMARAAAPAKVQVKVGLYTITYL
                     GLWYRGDALTHEQLIERAKKFGFEGIELDGKRPHGNPLDMPKKRCDEFRKRAQGEGVP
                     IYAVSGNNDFSSPVPEYRESQLVYMRELIKMTADLGAKTLRVFLAWPGVTVEPLPKGG
                     GRYDIAHPIWQFTHEKFSPEQTWAWCRQGLVESAKCAKDQGVTLALQNHRPVIDDYPD
                     VLRMVKEVDSPGLKVCLDAPLLKQKDETSVRKAALDVGSLQMLSHFGGEFDEAPDGSV
                     KGGEPYYKPFLKAMAEIGYTGYIGYELCHPLPVVDGKTVGLEFADKNARLAVKFMRGV
                     IAEANKELGAA"
     gene            12574..14883
                     /locus_tag="VLE22_16860"
     CDS             12574..14883
                     /locus_tag="VLE22_16860"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HSB16127.1"
                     /translation="MFFLRVSVFVVLLTTSLAFGQQPLYLDAKQPLNKRIEDLLSRMT
                     IEEKIGQMNMPCVYLSQLGRDVSAKLDACRRFTEGTYEKAIGPGGGFFTLADNALPGG
                     PREQAPYFNELQRIAIEKTRLRIPLLQTEEGTHGLMCSGGTIFPEGLAIGSTFNMDVV
                     EKIYVAAAREARAVGIHQIFTLVVEPNRDPRLGRNMEGYSEDPYLCSRIAERIVRGAQ
                     GDDISAPDKVVAGLCHYPGQSEPVSGLERGAMEVSERKLREVFLPPWVAGVKKTGALG
                     VMATYPAIDGVPAHASDWILTKILRQELGFDGLVLSEGSGIDTSVYERVAANQKEAGV
                     WSIKAGVDVGISYESGYMLDLIESAKEGKVSMAQIDRCVRRILRQKFRLGLFERPYVD
                     VNHAVNGLPRAEHRDLALQVAREGIVLLKNDKNLLPLRKDLKSIAVIGPNADHARNQL
                     GDYTSKSILQHIVTVLEGIKAKVSPQTQISYVKGCNVVGTAVNEIDKAREAAKKADVA
                     IVVVGENERRAPDGTNGEGKDVASLDLSGMQEELIRAVHETGTPTVVVLINGRPLSIR
                     WTAENVPAILEAWIPGEQGGQAIAEVLFGEYNPSGRLPITIPRHVGQLPVYYNHKPSK
                     AHWIKGTNYVDMKSSPLWTFGHGLSYTKFEYSNLQILPCEIGLAGEVTVTLDVRNTGG
                     RAGEEVVQLYLDDVVSTVTTPIKELRGFRKIALKPGESKRVEFNLGPDDLMLLNRDLH
                     WVVEPGAFDVMVGASSEDVRLRSSFEVRN"
     gene            14885..15451
                     /locus_tag="VLE22_16865"
     CDS             14885..15451
                     /locus_tag="VLE22_16865"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HSB16128.1"
                     /translation="MGVPARLCRREDSVLLVVDIQERLAAAMLPEAREQVIRNTGILL
                     EAAARLGVPRFLSEQYPKGLGPTEAGVLAKSGDGLFRFEKTCFSCRGEEEFSAALRQT
                     GRRQVVVAGMEAHVCVFQTAMELHAEGYEVYVAGDAICSRNLINRENALDRLLQAGLV
                     VTNTESVVFEWLRDSRHEQFRAISALVR"
     gene            15461..17068
                     /locus_tag="VLE22_16870"
     CDS             15461..17068
                     /locus_tag="VLE22_16870"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB16129.1"
                     /translation="MRKRMMLYTVLLAPAAILAVLHQPDAPAGRRTFHIDGTAGSDAN
                     DGRSPQKAWKTLDKVNGTVFEAGDRILFKSGTRYQGQLKPRGSGRFVNGRALPIVVDQ
                     YGAGNKPRIDAEGKFEAALHLYNVEYWEVNNLELTNLGAEPGVKRKGAFVQLEDYGSG
                     SHIHLKNLYVHDVNGTARKADGASGGIRWQTQGKVKNSRLNGLLIENCHIRRCERDGI
                     MGAGHRQRGVDWYPSLNVVIRKNLIEEVPGDGIVPIACDGPLVEHNVMRKCTRLLPEG
                     DAAAGMWPWACDNAVFQFNEVSDHKAPWDAQGFDSDWDCRGTIIQYNYSHDNEGGFLL
                     VCNNGGAPANIGVNDSTIVRYNISLNDGLRTQKTQSKGYFSPVFHISGPVKSTKIYNN
                     VVVVPKKPDVNIDRTMIYMDNWGGPWPEDTWFANNVFYVDGETKYVWGGSKNHRFEHN
                     VFFGTHTGGPDDPWAVRKDPLFAGPMTWGAGLGSLKGFMLRPGSPAIRAGLPVSFNGG
                     RDFWANRLPVGLPPSIGAHEFGTGGKD"
     gene            17078..19885
                     /locus_tag="VLE22_16875"
     CDS             17078..19885
                     /locus_tag="VLE22_16875"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HSB16130.1"
                     /translation="MKTSALVLTFIAATLSGCFTRDAASLKPATLRCEYRLNPAGIDE
                     TRPRLSWIVEAVQPQARGLKQTAYRIIASSSREKLVAGAADLWDSGRVASGSTTQITW
                     RGKPLGSRAEVYWRVMVWDEARRGSAWSEPAYFTMGLLSAADWQGRWIGKEESIELPK
                     PTKDKQQDQELEIRALRLPARMARKEFQLTQPVRRAVAYVSGIGLFELHVNGRRAGDH
                     VLSPNLTQYDKRVFYVTHDVTPLLQQGANAVGLLLGNGRWWAPRLRQPVPMVMYGTPR
                     AIAQIEIEHTDGSRSVVATDGTWRVTDAGPIRANNEFDGEEYDATLEMPAWTMAGFDD
                     SKWQSAVPVKPPDGRLVAQMAEPLKVIETLKPKSVKELRPGLFIADMGQNMVGWCRLK
                     VRGPQGATVQLRFAETLQPDGTLYTANLRSALVTDRYTLKGTKSVETWEPRFTYHGFR
                     FVEITGFPGKPTAESIEGRVVHDAMTPMHDFTCSNDLLNQIHRNIVWGIKGNYRSIPT
                     DCPQRDERQGWLGDRSMVSRSEAYIHDVAAFYSKWEQDIEDSQRPAGSVPDVAPSYWQ
                     FYNDSVTWPGTFLLVPGMLYEYYGDMRAIERHYPAMKKWVMYMRGFMKDGLIGKDSYG
                     DWCVPPEDPKLIHSKDPARITDKTLIASAYFIHLERQMARYGRLIGKGNEAAEFEDHA
                     AQMQTAFQRRFYKPEWGLYDNGTQTSSVLPLSFGLTPESERAKVFGNLVTRIGSESEN
                     HIGVGLIGAQWLMRALSDNGRADLAYTMATQTTYPGWGYMISKGATTIWELWNGDTAD
                     PAMNSGNHVMQIGDLGIWLYAYLAGIRPDIEKPGFAHVVLKPVMPGDLTFVRASHASP
                     RGRIESYWRKNGARVEWDVTLPPNSTATIYVPARSGNDVTESGASAAKAQGLRFSRME
                     NGNAVFEAASGRYRFQSVTAQ"
     gene            complement(19907..20314)
                     /locus_tag="VLE22_16880"
     CDS             complement(19907..20314)
                     /locus_tag="VLE22_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16131.1"
                     /translation="MRKRLAEPIPLHKLAPPMEIQVAPKRDFQKQERFNPFLLAGQES
                     GFEMFHGHCGGHVELKIQERVRRLLSLPIATKQWLRFVCTTCSAEYKVELTGRQDLSD
                     ECSFAQQCLRRALTEKQGSYGSTAHLSFSYRKP"
BASE COUNT         4046 a         5957 c         6696 g         3787 t
ORIGIN      
        1 cgaaagcctc ggatatcccc ctgaattgtt agtaccactt ccggccgaaa gcgtggaagc
       61 gttctcgggt gtctccaatg tgtcgttatt cgccgagatt cccagaggca gcgttgttct
      121 ggacttgggg tgcggcgcgg ggctcgattc agtcgttgct gcacgccgca cagggcccgc
      181 gggaagggtg attgggatcg aattcagcga ggagatgagc agcagagcgc aacgcggcgc
      241 ggcggctgcg ggcttccgca actggcttgt ttgccaggcg gatgccgagc ggctcccgat
      301 cctcgacaac gcggtagacg ttgcgcttgc caacgggatc ttcaatctga atccggcgcg
      361 gaaggccatt ttgcgagagc ttgcacgtgt gttgcggcca ggcggggcag tgtacggggc
      421 ggagttgatc ctgtccgagc cttgccggcg gggcggcaga tgaccgaggc cgattggttt
      481 gcctgaatcg ccggtgcaaa agagggcggc gccttcctcg atgagttccg ggcggcggga
      541 ttcagcgacg cgtgtttcct gagggtctcg cgtaacgcgc ggacgaagga cccaagggtg
      601 ctggccgcgg agttccgggc ggtgaagtga tgagcacctg cgcaatgcct gctacgccgc
      661 aggcacaccc cgacgacgcc gttgatatat tcggctcgcc tgcacaccct tggccggatc
      721 ggctgagtgg gggctaccgt ttcttcaacg acagcgacat cccgcgatcg tgcatgtgaa
      781 tgaaaacagt gtccagcttg ggattcgtcg tcaccgcctc cacgaattcc ggccgcgcgg
      841 tggggtaccg catgttgtgc gcgacgatca ttccgccagg gcgcacgaga ggcaagacct
      901 gctccagata ttgccggtag ccttctttct ccgcgtcgat gaacaccaga tccaccggac
      961 ccttcacctt cgccagttcc ttatgagcgt caccctcaac cagcgtcaca acggtctcga
     1021 ctcccgcctt cttgaagttg tcacgcgcga tcgccgcccg atccttatcc agttcgaacg
     1081 tcatcagccg cccgccggtc ttgcgcagcc catttgcgaa ccatagcccg gaatagccgg
     1141 tagaagtccc gaattccagc actgtcttcg ctccggagaa ctccgccagc atccgcagga
     1201 cccgcgcgtc gctcaaggtt acgttggcga agcggggcgc cgcctccgcc acttcgatga
     1261 tcttcttctc ggcctccgat tcgggtaacg gaaatcgcgc gagcgcctcg ccggcgaagt
     1321 ccgatcggtg agccgcgatg aggagccaac ggccgttcgc tttctgccag gtacgcatga
     1381 accgctggcg caccatctcg ggagcgtggt tttctctctg ccgttcgact cgctcgactc
     1441 cgcagacgat gccggtgtct ccgatgacgc gatagagggt tccttcctcc ggctgaacgc
     1501 tccacgattt gaacggtgag aacaatgccc ggacaacttc gcgccgctgc tccgacgacc
     1561 gggcggccca atccgcaggt gcctccgcgt ccacgctcag gtacacgaac tccttgtgga
     1621 acctgccgag gatttcctcc gcgtcatgcc ggttcagggc ttcgagtagc ccgtcgtctg
     1681 cggtctttag ctcctctgtt ccggatggcc aagcggcccg ggaaagcagt attgtcccgg
     1741 caatcagcga aagcgccaat tcactgatat tcatcccctt ttcctcctga gtcttgtgaa
     1801 gtgatgtgcg atctctgtcg cgacctctga cctggtcaca cgccagatgc ccagtccggg
     1861 ccagtccaca gataaccgcg gagcccgaat ttcccttatc ttaagtcacc cagaattccc
     1921 ggtgcgctcc ggtgctgatt gtcaggcttc gatccagttg ctgcgctcga taatggttgg
     1981 cggttcgtag ctgtggcccc tggccaggcg cttgtcctca cgaatgctgg tccaccagag
     2041 gacgggtccg aggagtcggg cagtggcggc ggtcagcacg ccgaactccc gctcgaggtc
     2101 cttgcgcaga gcgcggatct gcagggtcac ggagtggttg gtccgcttga gccggtgctc
     2161 catcgcccac aggattccgg cgtacacacg ccgcagcgag ctcgcctccc gctcgaatcg
     2221 ttccctgacg cgcaggtctg ggtgattcct gtgccgcttc cagccttcga acgtggtccg
     2281 gcagatgcga aacaggctcg gtccgttgcg ttcgaagtcc cgcaagaaag cccagtcaag
     2341 aagccgcttg gactcctccc gtgagatcgc cgcatgctgg aagttgaagc cgaactggcc
     2401 gtgaatgtcc gcgagatcga cgtcgagcag ctttcctcgt tcagccatgt cgcgatagag
     2461 cggcgtgccc ggcacggggg tgtagagcat gaactggtgc aagtcggtct cgtgcgcgat
     2521 ggcgtgctcg atttcctcgg tgatgttctc cggggtgtgg tgctccagac ccagaatcgt
     2581 ggagccgagc aacatgatgc cgtgcccgcg gagttcgcgc gccatgcgca gcgtgtcggc
     2641 gccgtcgagc ttggagtagt tcgagcgcgg cgattcgagg ccgagccaga tcgaggagac
     2701 gcctaactcc accagctttt cgcaactgta cttccgaatc gcattcgcgg aggaaaagat
     2761 gttgagcgcc cagctcttcc gggcctgctt catcctggca agcaagtcca tggcgcgttc
     2821 cttttgcagc aggaagttct cgtccatgat gaagaatgac ttcacattcc gcgaggtctc
     2881 agcctcctcc atcacccgga acaattcctc acccgtggaa tagaagttga gtactttgcc
     2941 tttcccgccg aagaacgccg aggttgtaca gaagttgcag cccatcgggc aacccaccga
     3001 cgcgatcaca gtggcggaac tgtcggtttt ctcggggact ttgatgccca tcacacgcag
     3061 gtcgaacccg gaggagagcg cgggatgaag gatgggcgca tcgacatcct cgccgagata
     3121 ccggcgcatc cacgcgatgc cgtcgccctt cacgatatgg tcggcgtcca ggatgtgctc
     3181 cacgccgggg atggccgtga catgcccgcc gacgacgatt gtcgaatgcg gcgagagctt
     3241 ccgcaccatg cggcacatct cgcgagcttt gcccacgttc acaatgatcg aggagatgcc
     3301 gaccacgtcg tactggttcg tggagagctc gcacgcgaaa gcctctcggg tcgggaaatc
     3361 cagcactgtg cagggcgccg agatattctg ctggatcatc atgatgcccc acgaccggtg
     3421 gaagcggcgc aaggaaaacg atccctgcgc tcttgtcacc tggttgtggt agagctccat
     3481 cgggttgatg gcgcggctgc cgaattcatc gtcccgggcg tacgggccga agacggaact
     3541 gagcaggata cgggtacgcg acccgttcgg gtggggatca atcatcgtgg agaaggctcg
     3601 acggaaaagg ttcccttacg cttttgttcc agcgggcgtg aaaaccggct gtctcgcggc
     3661 aggtccgcgg aaacactgcg gggagtcagg cgaacgttca gtacacgctc gatgcggcgg
     3721 atttcattgc gctcgttgcg cgtgctgaac gtggaggcgg tgccgcgggc gccggcgcgg
     3781 ccggtgcgtc ccacacggtg gacaaagtcc tcgggtacgt gcgggagatc gtaattcacc
     3841 acgtgagcga tgccgtccac atgaatgccg cgggcggcca catcggtggc caccaggacg
     3901 cgatactgcc cgtccttgaa tccgttcagt gcctggttgc gctggctttg cgttcggccg
     3961 ccgtggatgc tggcggtctt tacaccggtg tgggaaagct ttttcgccaa gcggtccgtg
     4021 ccgtgctttg ttcgcgcgaa cacaaggaag gatcccggct cctgtccgat cagccgggtg
     4081 agcaggccga gtttgcggtc tgactcgacc tcatacacgt gtaggttaac ctggtcggcg
     4141 ggcttggtgg aaggcccgat ggctacgcgg acgggattgc gtacgtacgc ctgaatcaga
     4201 tgcgccaccg attgttcgat ggtggccgag aagagcagag tctggcggga ggccggcacc
     4261 accttcaaaa tggcgtggag ggcgggcagg aatcccatgt cgagcatgcg gtctgcttcg
     4321 tccaacacga agaagtgcac gccgctgagg ctgacgaggc ggcggtccag aaaatcgcac
     4381 agacggccgg gtgtggcgat caggacctga gcgccttgcc ggatagctcg cagttgaccg
     4441 gcttcactca tgccacccac cgccacggcg gaccggatac cagtgcctgc ggttatgcgg
     4501 ctgaacgatt cgttggtctg catggcgagt tcgcgagtgg ggctgaggat gagggcgttg
     4561 atgccgctct tgcagcttcc gcccatcagc gactgaagca acgggaggag aaacgccagg
     4621 gtcttaccag ttccggtttg cgcggtggcc accacatctt ggccggcgag cgccggcggg
     4681 atcgcccggg cctgcacggc agtcggctgg acaaagccct gctttgccag attgctctgc
     4741 aaccgagagc acagagagag ttcagagaaa tttttcattc agagttttgc ggaagaggaa
     4801 tcagctagcc gggaaaaaag gagaggtttt gcggatcggc ttccagtacg cttccagaat
     4861 agcgcagttc cggcgcgaat gctcgccgtc acttgccgct cggggtcatc ccccgctgct
     4921 cgcggccaac gaagtgatac cacctgtttt tggcttatgc tcgctccgga accgcgccgg
     4981 cggtgtgccg aacgcgcgct taaacgcgcg gttgaaagcg gcttcggatt cgtatcccac
     5041 ctcagcggcg atctgcgcca cactgcgcgg ggtggacatg aggctgcggg cggccaattg
     5101 cagcctccac cgtgtgagat acgccatcgg gggctccgag aggtagcggc tgaagcgctc
     5161 caccaaagcc gagcgtgaca aaccgacttc cttggctagc tgggcgatgg tccacggctg
     5221 ctccacccgg ctgtgcaaga tcgcaagact cttgccgact acagggtccc gtgtaccagc
     5281 aagccacccg gtctgctccg ccggcaaagc ggcaacatag cggcgaagtg tatcgacaaa
     5341 gagagcttcg gagagtttcg ccagcatggc ttcgctgccc ggctggccgg cacccgcttc
     5401 atcgacgaga tggcggatgc agttctcgag ccatcggccg gagccgtctg cgcggatgtt
     5461 gactttcatc atgcggggca gcccgctcag aatcgggcgg aacaggtgcg gttcgcaggc
     5521 catgtaaccg caaataaacc ggctgacctc accaccgcca ccagtgcgca tcagcccaag
     5581 gcttcgcctt cggatcagcg gcaagatatc ggagatctct atggattgtt cccgcgcgct
     5641 gctggccatc ctgtgcggat cgccgtgcgg aaagatgaca atgtcgccgg cgttgaggct
     5701 gagcagattg ccaccttcca attgtgcgta agcggagccc tcgatcacga agtgatagat
     5761 gatcaaatgt tcggcgcctg ggacgaggtg gggcgccaag caatcggagc gcggcgaggc
     5821 gacgctccag ggagccgaaa gctccgcgtt gaagaacagt gcgcccttca gcttgacacc
     5881 gttcaggatt tccgagaacg cgtccaaacg gcccctccgt tttctctgca aagtctcgtg
     5941 gacgcccggc aaacctcaat gctaccactg gactccagag caaagactcc gggcgctaag
     6001 gcatggccga accgcgccca tttacccaca attatcttga ccacacagcc tgcgcgcaac
     6061 tgggtggctg ttgtgggtca gagggagaga ccgatgaaaa aagccctttc cgttacctat
     6121 ggcgccctgg cttacgtgat gttcctgggc gcctatttgt acctgatagc gtttaccgtg
     6181 aatgctgtcc cgaattccat cagcggaccg acatcgctgc cgcctctgtt ggcggcagcc
     6241 attgacctgg ggctcattac gatcttcggt ttgcagcaca gcatcatggc gagacccgga
     6301 ttcaagcgcc tttggacgcg gatcgttccg ccacacctcg agcgcagcac ctacgtggtg
     6361 atcgggtcgg tgatggttgt cttgatcgtg gtgtgctggc agcccatcgg cggcgagatc
     6421 tggaacgtga ccggcgcagg catggttgcc ctgtacgcga tcgcggcgct tggatggctc
     6481 gtggttcccg tcaccagctt tcttaccgat cacttcgagc tgttcggcct gcgccaggtc
     6541 ttcgagtatg cgctcggccg gccgaagtcg aagcccatgt tcaaggtgcg ggccttttac
     6601 aggaaggttc ggcatcccat gatgctcggc tggatggtgg ggttctgggc gacgccctac
     6661 atgacaacgg gtcacctgtt gtttgcggga ctgatgacgt cctacatcct ggttgccatc
     6721 cattttgaag agcgggatct agcgcgcgag cacggtccgg cgtatcgcgc ctatcaggct
     6781 cgggtgccca agttggtgcc gttcagcaaa ccggcgggag cggaacaggg tgcgttcgaa
     6841 ggaatggtag gagaaccgca gattcagtga acgcagggga gaagccacgt tccactgatg
     6901 tccaatcctt tcagtcgtgg ctcggcaact gcgtgcggtt ggtagttaat ctcctagcgc
     6961 cggctcagcg gccaagcgtc gacccgacag ccagactctc ccgtcttcga ctaacgagta
     7021 gccgacgggc acgaccaata gggtgagcaa caggcagagg gtctgtcctc cgatgatggt
     7081 gacggcgatc gcggaccgct gcgaggcgcc ggcgccgatg ccgattgcgg tggggatcag
     7141 acccgccaca atcgagaagg tcgtcatcag aatcggacgg agccgcacgc ggttggcttc
     7201 gaggatcgcc tcgcggagcg gttggccctg ctgccggagg cgattcatgt agtcaatctg
     7261 aagaatgccg ttcttcttga caatgccaag cagcagcagc acaccaagag cgctgaacag
     7321 gttcaggctc cgccccgtgc cgatcagcga aatcaacgcg aaggggatcg acagcggcag
     7381 ggtgagcaga atgatgaacg gatggaccag gctttcgaac tgggctgcca gcaccatgta
     7441 catgaagatc gaggcgaggc cgatggccag aatcatgttg gctgtggtct cctcaagaat
     7501 cttcacctgg ccggagaaac gcatctcgta tccggccggc attccgactc gcgcgatggc
     7561 ttcctgtgtc tgcgcggcgg cttcgcccag ggaacggccc ggcgctacgt tgccgtaaat
     7621 gccgaccgcg aactggcggt tgaaccggtc gatgcggctg ggacctagcc cgcggtccac
     7681 atgggccacg gaatcgagcc ggatcagccc gagtcttgca gaaggcacaa gaagacggct
     7741 cagtgcgtta gggtcgtctc gctgcgaggg caggaggcgc atcgtgaccg gatactgttc
     7801 ggagccttcc ttgaaggtgg agatctcatc ctcgccggac atcagcagcc tgacggcgct
     7861 ggcaatgtcg gaaacgcgga caccaagatc ggatgccagc gaacggtcta tgtccacctg
     7921 gaattcggga ttgctgaggt tgaggttcac tttgatgtcg gccacggcgt cggcatgcgt
     7981 caattcgatg cttgcctgtt ttgcgagctc catcactttg ccgaggtccg gaccgagaag
     8041 ctgagcgcga atcggagaga acgtctcgtg cccgcccaac gcgttgggaa aggtcacgcg
     8101 ggggcgagta taaggatagt cgcggagcac ggcccgcacg tgctgtccca tctgctcgat
     8161 actctcacgc tcgctcacgt ccttgagcag aatggtcatc cacgccatcg tcacgcggtc
     8221 cgcgaaggac gaggtctgcg gcacgacggt gaccacgcct ggtattttgc ggatctttcg
     8281 agagatctcc agggtcacct tctcggtcgc ttccagggac gccccctccg gcatttcgag
     8341 catcaccgag agctcgttct ggtcttcctg cggcatccag tcgcgcccga tgtgtctgct
     8401 gagcaggaac gttgaggcga aggtcagcac gctgaccgac acgatgaccc agcggtgatc
     8461 gagagaccac ccgagcgtct tgacgtagat ccggccgaga acgccgtggt gatcctcggg
     8521 acgcgccgca gccccgttgc ccgtccgccg ccgaaggaga cgcgagctta gcgatggcgt
     8581 cagcgtgaag gcgacaagca tcgaaaccat gatggccatg gccatggtcc agccgaactg
     8641 gttcaaataa cgccgcgcgt aaccctgcat gaacgccacc ggcacgaaga tgatcaccag
     8701 agacagcgtg gtcgccatga cagccagggc gatctctttg gtggctttga cggccgcctc
     8761 catcggcgac atcaatttct cctcgatgaa gcggtagatg ttctcgagaa cgatgatggc
     8821 gtcatcgatt acaatgccca cagccagcgt gaggccgagc agcgtcatcg agttcagggt
     8881 gaagtccatc atcttcatca gcgtgaacgt cgtgatgatg gacgtcggaa tggcgataga
     8941 gctgatcagg accgtgcgcc agttgcgtat gaacagccac acaatcagcg acgccagcag
     9001 acttccgatg accagatgtt cttccagcgc cttgacggag ctcttgatat acgtagcctg
     9061 ctctttcact acttcgagct tcactccggc aggaagttcg cggccgagcc tctcgactcg
     9121 cgctaggatg ccgtcgacga cctgcacggt gttggtacca gtctgccgcc gggcctcaag
     9181 ggtgacagcg ggcttgtcct ggtagtaggc aaacgtgcgc ttctcggtca tgctgtcttc
     9241 ggcatatccg acatcgcgaa ggcggatggg cgctccgccg acgttcttga tgatgatgtc
     9301 gttgaattca gcgaccttct cgaggcgtcc tagcgtgcgc acgcccatct ccgtaggtcc
     9361 gcgcacgatc cgcccgccgg gactctcgat gttttccgct ttgatggcgc gctcgatatc
     9421 ctgggcggtc aggttgtaac cggagagttt gtcaagatcc aggagcacgt tgacctggcg
     9481 gttctgccca ccggcgatgc tgatgctggc aaccccgtcg acggtctcca gcgcccgccg
     9541 aacctgcttg tccgcgatct cggtaagttc gcgaatcgaa aggtcaccgc tcacggccaa
     9601 ggtgacgatc gggtcgctgt cgggatcggc tttctgcact acgggcggga ggacgttggg
     9661 cggaagccgc cgcattgccc cgctcacctt ttcgcggacg tcctcggcgg cttcgccgat
     9721 gtccttttcc agtacgaagg tcacgataat gttcgccgtg ccctccaaaa cggaggcgcg
     9781 catctcgtca atcccgctga ccgaggcaac ggcttcctcg atcggcagga ccacctggga
     9841 agtcatttcc tcggggctgg cgcctggaag acggactcgc acgtagacag tagcgggatc
     9901 tgtccgcgga aacagatcca ctcccagtcc catgaagctg aaggcaccca tgacgaccag
     9961 aaacatgatc agcatgaacg caaacacggg gcgctggacg caaatttcag aaaggcgcat
    10021 aagacccttg ctccgaattc ggtacagtct gattgtagcg ctgcaccgaa ttcctgcgat
    10081 aactagaata gatgtgtcgc gagcaatcaa cgatatgcgc aatagataca gttacgttgc
    10141 cgggtgggtg accgtcattt tcgtgctggc ggtattcgcc ggctgccggc caaaaccggc
    10201 ggagaccaag aagcagaccg gaccggtaga ggtccatgcc gcgccagtcg tgcaccggga
    10261 ggcgcggaga gtggtcgatt cggtggggac gctgtttccg ttcgatgaag cgatcattag
    10321 cgcggaaatc gaaggaccgg tggagaaggt caacgtggat ctgggcgacc gcgtggcgca
    10381 aggacaggtc atggtgcaga tctccgacga ggagcagcgc tacctggtcg cgcagatgga
    10441 ggcgcaactg cgccagtccc tcgaacgtct tgggcttaag gaagagaacg agaaggtcaa
    10501 ggacatccgg ctgacgccgg aggtaaggcg cgcgcaggca gatctgacgg atgccgatca
    10561 gcgtctgcgg cgcgtgcgca gtctcgtgca gcagggaatc gggtcgcagc aggatctcga
    10621 cgaggcccag gcgcggttcc agtcgcttca ggcagcctat gacgccatgc tgaatcagac
    10681 ccgaaaccta gtccgggacg tagagcgcac gcgcgccgtg ctcgatctcc aacgcaagaa
    10741 gctgcgcgac acccaggtcc gtgctgcgtt tgctgccaac gttaaggagc gccaggtcac
    10801 cgtcggccag tatgtgcggc caaacacgcc cttgcttacg ctagtcaaga tagaccccat
    10861 ccgccttcgt gccgaggtcc ccgaacggat ggccccatgg atcaaggtcg gccaggtggc
    10921 cgaagtgtcc gtcgaggcgt tcgagaatcg gaaattccgc ggcaaaatct ggcggatttc
    10981 accgaccgta gatcaaagca agcgcacgtt tctggttgag gctttgatcg acaaccgcag
    11041 cgaggaactc aaaccgggct cctatgcgcg cgtgagggtg gaaacagaca aggtcgagaa
    11101 aatccggctg atcccggtga gcgctatcaa ctatgtcttc ggactgaaca aggcctatgt
    11161 cgtctccggc ggcgtcgtgg aggcacgcga cgtaaaactg ggcgaccgtt acggccagga
    11221 agtcgagatc accgagggcc tcgaagatgg ggagatggtg gcaacgtccc aggtggcccg
    11281 gctggacacg ggcgtaaagg tccgcttggc gtcggccgcc ggaaatcagc gggtgaacta
    11341 gaacaaggtg ctgaaaaacc cgcgccacag gctgaagccg cgccaccatt ggacctcgac
    11401 acagtacgtg tggcagtagc acagcccgga gcctgaaggg tgtatattgt ccgcggggcg
    11461 gttgtgtacc gcttcggagg caagcgagat ggacaacgtg atgactcgac gttcggcgat
    11521 cggcgcggga ctgatgtccg gcatggcggc cggcatggcc cgggccgccg cgcctgcaaa
    11581 ggtgcaggtg aaggtggggc tctacactat cacctatctg ggcttgtggt acaggggcga
    11641 cgcgctcact catgaacaac tgatcgagag ggcgaagaag ttcggtttcg agggcatcga
    11701 actggatggc aagcggccgc acggcaaccc gctcgacatg cctaagaagc gctgcgacga
    11761 gttccgtaag cgggcgcagg gagagggcgt cccaatctat gcggtctccg ggaataacga
    11821 cttcagcagc ccggttcccg aataccgcga gtcgcagctt gtttacatgc gcgagctgat
    11881 caagatgacc gcggatctcg gagcgaagac cctccgcgtc tttctggctt ggccgggtgt
    11941 gacggtggag cctcttccga aaggcggcgg gcgttacgac atcgcacacc cgatctggca
    12001 gttcacccac gagaagtttt cacccgagca gacctgggcg tggtgccgtc agggactggt
    12061 cgaatccgca aagtgtgcga aagaccaagg cgtgacgctg gcgcttcaga accatcgccc
    12121 ggtgatcgat gactacccgg acgtgttgcg gatggtgaag gaagtcgatt cgccggggct
    12181 gaaggtgtgc ctcgacgctc cgctgctgaa acagaaggat gagacttcgg tccggaaagc
    12241 agccctcgac gtcggctcgc ttcaaatgct gtcccatttc ggcggcgaat tcgacgaggc
    12301 gccggatggc agcgtcaagg ggggagaacc gtactacaag cccttcctga aggccatggc
    12361 ggagatcgga tacacgggct acatcggcta cgaactgtgt cacccgcttc cggtcgtgga
    12421 cgggaagacg gtcggtctgg agttcgccga caagaacgcg cggctggccg tgaagtttat
    12481 gcgcggagtt atcgcggaag caaacaagga actcggcgcg gcgtaaaccg ggcccggtcg
    12541 atcaaaacaa ggaggcgtgg agtgaaggtg tgcgtgttct ttcttcgagt cagtgttttc
    12601 gtggttctcc tgacaacatc gctggcgttt gggcagcagc cgttgtatct cgatgcgaag
    12661 cagccgttga acaagcggat cgaagatctg ctctcacgga tgacgatcga ggaaaaaatc
    12721 gggcagatga acatgccctg cgtctacctg agccagttag gacgcgacgt gtctgccaag
    12781 ctcgatgcct gccgccgctt tacggaaggc acgtacgaga aggcgatcgg cccgggcggc
    12841 ggcttcttta cgctcgcgga caacgctctg ccgggcggtc cgagggagca ggctccgtac
    12901 ttcaacgaac ttcagaggat cgcgattgag aagacccggc tcagaatccc actgctccag
    12961 accgaagagg gaacacacgg cctgatgtgt tccggcggca cgatttttcc cgagggccta
    13021 gccatcggca gcactttcaa catggacgtc gtcgagaaga tctacgtggc cgccgcacgg
    13081 gaggcgcgag cggttggcat ccaccagatc ttcacgctgg tcgtggagcc gaaccgggac
    13141 ccgcgcctcg gtcggaacat ggaggggtac tccgaagacc cgtacctctg ctcccgtatc
    13201 gcggagcgga ttgtccgcgg ggcacagggc gacgacatca gcgcgccgga caaggtggtc
    13261 gccgggttgt gccactaccc cggtcagagc gaacctgtca gcggtctcga gcgcggcgcc
    13321 atggaggtct ctgaacggaa gctgcgggaa gtcttccttc caccctgggt ggcgggcgtc
    13381 aagaagacgg gcgctctcgg cgtcatggcg acataccccg ccatcgacgg agtgccggcg
    13441 catgcctccg attggatact gacgaagatt ttgcggcagg aactcggctt cgatgggctg
    13501 gtgctcagcg agggcagcgg catcgacaca agcgtctacg agcgcgtggc agccaatcag
    13561 aaggaagccg gagtgtggtc gattaaggcg ggcgttgatg tcggtatctc gtacgagtcg
    13621 gggtacatgc tggacctgat cgagagtgcc aaagaaggaa aagtctcgat ggcccagata
    13681 gaccggtgcg tgcgaaggat tcttcggcag aagttccgcc tcggactttt cgaacgcccg
    13741 tatgtcgacg tgaaccacgc cgtgaacggg ttgccccgcg cggagcatcg tgaccttgca
    13801 ctccaggtgg ctcgcgaggg catcgtgctg ctcaagaacg acaagaacct tctgccgctg
    13861 cgcaaggacc tgaagtcgat cgcggtgatc gggcccaacg cggaccacgc acgaaaccag
    13921 ctcggcgact acacgtcgaa gtcgattctg caacacatcg tcaccgtgct cgaaggaatc
    13981 aaggcgaagg tctcaccaca gacgcagatc agctacgtga agggctgcaa cgtggtcggt
    14041 acggcggtga acgagatcga caaggcgcgc gaggcggcga agaaggcgga tgtcgccatc
    14101 gtagtggtcg gcgagaacga gcggcgggcg ccggacggga cgaacggcga gggcaaggac
    14161 gtcgccagcc tggacctcag cggcatgcag gaagagctga tccgggcggt ccacgaaacg
    14221 ggtacgccga cggtggtggt gctgatcaac ggcaggcctc tgtccatccg ctggaccgcc
    14281 gagaatgtgc ccgcgattct tgaggcctgg atcccgggcg agcagggagg ccaggccatt
    14341 gccgaggtgc tgttcggcga gtacaacccg agcgggcggc ttccgattac gattccgagg
    14401 cacgtgggac agttgccggt ctactacaac cacaaaccgt cgaaggccca ctggatcaag
    14461 gggacgaatt acgtcgacat gaagtcgtcg ccgctatgga cgttcgggca cgggctgagt
    14521 tacacgaagt tcgagtactc gaatctccag attttgccgt gcgagatcgg gctggcgggc
    14581 gaagtgacgg tcacgctgga cgtgaggaac accggcggcc gcgcgggcga ggaggtcgtg
    14641 cagctttatc tcgacgacgt cgtgagcacg gtgactacgc cgatcaagga gctacgcggc
    14701 ttccggaaga tcgcgctgaa accgggtgag tccaagcggg tggagttcaa tctgggcccg
    14761 gacgacctga tgctgctcaa ccgagacctg cattgggtgg tcgaaccggg ggcgtttgac
    14821 gtgatggtgg gtgcgtcgtc ggaggacgtc cggctgcgga gcagtttcga ggtgaggaac
    14881 tgacatgggt gtacctgcac gtttgtgccg gagggaagat tccgtgctgc tggtggtgga
    14941 catccaggaa cggctggcgg cggccatgct ccccgaggcg cgcgagcagg tgatccggaa
    15001 caccggcatc ctgctcgaag cggcggcgcg gctgggagtg ccgcggtttc tcagcgagca
    15061 gtacccgaag gggctcgggc cgaccgaggc aggcgtgctc gccaaatccg gagacgggct
    15121 gttccggttt gagaaaacct gcttctcgtg tcgaggcgag gaggaattta gcgcggccct
    15181 gcggcagacc ggcaggcgcc aggttgtggt ggcgggaatg gaggcgcacg tgtgcgtgtt
    15241 ccagacggca atggagttgc acgccgaagg gtacgaggtg tacgtagccg gtgacgccat
    15301 ctgttcccgc aatctcatca atcgggagaa cgcgctcgac cggctgctgc aggccggatt
    15361 ggttgtgacg aacacggagt ccgtggtttt cgagtggctg cgggattcgc ggcatgagca
    15421 gttcagggcc atctcggctt tggtgcgata ggagaattcg atgcgaaagc gaatgatgct
    15481 gtacaccgtc ctgctggctc cggctgcgat cctggcggtg cttcatcagc ctgacgcacc
    15541 cgcagggcgg cgcacttttc atatagacgg cacggcaggc agcgacgcga acgacggccg
    15601 ctcgccgcag aaggcgtgga agacgctgga caaggtgaac gggactgttt tcgaggccgg
    15661 agaccgcatc ctgttcaaat cggggacgcg ctaccagggt caactgaagc cgcgcggctc
    15721 cgggcgattc gtgaacggcc gggcgctgcc gatcgttgtg gaccaatacg gagcggggaa
    15781 caagccgcgt atcgatgccg agggcaagtt cgaggcggcg ctccatctct ataacgtcga
    15841 gtactgggag gtgaacaacc tggagttgac gaacctcggc gccgagccgg gcgtcaagcg
    15901 caaaggtgcc ttcgtccaac tggaggatta cggcagcggc tcgcacatcc atctgaagaa
    15961 cctctacgtt cacgacgtca acggcacggc gcggaaggcc gacggcgcga gcggcggcat
    16021 ccgctggcag acgcagggta aggtgaagaa ctctcggctg aacggcctgc tgattgagaa
    16081 ctgtcacatc cggcgctgcg agcgtgacgg aatcatgggc gcgggccaca ggcagcgtgg
    16141 cgtggactgg tatccgagcc tgaacgtcgt aatccgaaag aacctgatcg aggaggtgcc
    16201 cggagacggc atcgtgccga tcgcctgcga cggcccgctc gtggagcaca atgtaatgcg
    16261 caagtgcacg cgcctgctcc cggagggcga cgccgcggcg ggaatgtggc cgtgggcgtg
    16321 cgacaacgcg gtattccagt tcaacgaggt gagcgaccac aaggctccgt gggatgcgca
    16381 gggcttcgac tcggactggg actgccgcgg caccatcatc caatacaact acagccacga
    16441 taacgaaggc ggcttcctgc tggtgtgcaa caacggtggt gcccccgcga acatcggcgt
    16501 caacgacagc accatcgtcc gttacaacat cagcctcaac gacgggttgc gaacccagaa
    16561 aacgcaaagc aagggctact tctcgcccgt gttccacatt agcgggccgg tgaaaagcac
    16621 gaagatctac aacaatgtcg tcgttgtgcc gaagaagccg gatgtgaaca ttgaccgcac
    16681 gatgatctac atggacaact ggggcggtcc gtggccggag gacacatggt tcgccaacaa
    16741 cgttttctat gtggatggcg agacgaagta cgtctggggc ggcagcaaga accaccgctt
    16801 cgagcacaac gtgttcttcg ggacgcacac cggcggcccg gacgatccct gggcggtgcg
    16861 gaaggaccct ctgttcgcag ggccgatgac gtggggagcc ggcctgggtt ccctcaaggg
    16921 attcatgctg cggcctgggt cgccggcaat ccgcgcgggg ttgcctgtct cgttcaacgg
    16981 gggtcgcgac ttctgggcca accgcctgcc tgtcggcctg ccaccgtcaa tcggcgcgca
    17041 cgagttcggg acgggtggga aggactagga gaatattgtg aagacctctg ctcttgtact
    17101 caccttcatt gccgctacgc tgtccggttg cttcactcgt gacgctgcct ccctcaaacc
    17161 cgcgacgttg cggtgcgagt accgtctgaa cccggctggt atcgatgaaa ccaggccgcg
    17221 gctgagctgg attgtggagg ctgtacagcc gcaggcgcga ggattgaaac agaccgcgta
    17281 tcgaattatt gcgtcgtcgt cgcgcgagaa actggtcgcg ggcgccgcgg acctgtggga
    17341 ttccggacgc gtcgcgagcg gttcgacgac gcaaatcacg tggaggggta agcccctcgg
    17401 atctcgcgcc gaggtctact ggcgcgtcat ggtgtgggat gaggcccggc gcggctcggc
    17461 ttggagcgaa ccggcgtact tcacgatggg acttctgagc gcggccgact ggcaggggag
    17521 gtggatcggt aaggaagaat cgatcgaact gccaaagcca accaaggaca aacagcaaga
    17581 ccaggagttg gagattcggg cgctgcggct tccggcgcga atggcccgca aggagttcca
    17641 gctcacacaa ccggtgcgca gggcggtggc ctatgtctcc ggcataggac tgttcgagct
    17701 gcacgtgaac gggcgtaggg cgggcgacca cgtgctgtcc ccgaacctca cgcagtacga
    17761 caagcgggtt ttctacgtca cccacgacgt gacgccgctg ctgcaacagg gcgcgaacgc
    17821 cgtagggctg ctgcttggca acggtcggtg gtgggcgccg cggttgcgcc agccggtacc
    17881 catggtgatg tacggtacgc cgcgcgcgat cgcacagatc gagatcgaac atacagacgg
    17941 ctcacgctca gtcgtggcta ccgacgggac gtggcgcgtg accgatgccg ggccgattcg
    18001 ggccaacaat gagttcgacg gcgaagagta cgacgccacc ctggagatgc ctgcctggac
    18061 aatggcggga ttcgatgact cgaagtggca gtctgctgtg cctgtgaagc ctccggatgg
    18121 caggcttgtg gcgcagatgg cggaaccctt gaaggtgatc gagacactga agccgaaatc
    18181 agtcaaggaa ctgaggccgg gcctgttcat tgcggatatg gggcagaaca tggtgggctg
    18241 gtgccggttg aaagtgcggg gcccgcaggg ggctacggtc cagttgcggt tcgctgagac
    18301 actgcaaccc gacggcacgc tttacactgc gaacctgcgg tcggcgctgg taacagaccg
    18361 ctacacgctg aagggaacga aatcggtgga gacgtgggag ccacggttta cctaccacgg
    18421 gttccggttc gtagaaatca ccggatttcc gggcaaaccg accgccgaat cgatcgaagg
    18481 ccgcgtcgtg cacgatgcca tgacgccgat gcacgacttc acgtgttcga acgatctctt
    18541 gaaccagatc caccggaaca tcgtgtgggg gatcaagggc aactaccgtt ccatcccgac
    18601 cgactgtccc cagcgcgacg agcggcaagg gtggctcggc gaccggtcga tggttagccg
    18661 tagcgaggcg tacatccacg acgtggcagc gttctattcg aaatgggagc aggatatcga
    18721 ggattcgcag cggccggcgg gcagcgttcc cgacgtggct ccgtcttact ggcagtttta
    18781 caacgactcg gtgacctggc ccggaacgtt cctgctggtg ccgggaatgc tgtatgaata
    18841 ctacggcgac atgcgggcca tcgagcggca ctatccggca atgaagaagt gggtgatgta
    18901 catgcgcggg ttcatgaagg acggcttgat cggcaaggac agctacggcg actggtgcgt
    18961 gccgcccgaa gacccgaagc tgatccactc gaaggacccg gctcgtatca ccgacaagac
    19021 gctgatcgcg tcggcgtact tcatccacct ggagcggcag atggcccggt acgggcgtct
    19081 gatcggcaag ggcaacgagg ccgcggagtt cgaggatcac gcggcgcaga tgcagacggc
    19141 cttccaacgc cgtttctaca agcccgaatg ggggctgtac gataacggga cgcagacgtc
    19201 gtcggtactg ccgctgtcgt ttggtttgac gccggagagc gaacgggcga aggtgttcgg
    19261 caaccttgtg acgcggatcg ggagcgaaag tgagaaccat atcggcgtcg ggctgatcgg
    19321 cgcgcagtgg ctgatgcggg ctctttccga caacgggcgc gcggacctcg cttacactat
    19381 ggcgacccaa acgacgtacc ccggctgggg ctacatgatc tcgaaaggcg ctacgacgat
    19441 ctgggagctg tggaacggcg acacagccga cccagcgatg aactcgggca accacgtcat
    19501 gcagatcggg gatctcggca tctggctgta cgcgtacttg gcgggcatcc ggccggacat
    19561 cgagaagccg gggttcgccc acgtcgtgct gaagcccgtg atgccggggg atctgacgtt
    19621 cgtacgcgcg tcgcacgcct cgccgcgggg ccggatcgag agttactggc ggaagaacgg
    19681 ggcccgggtg gagtgggatg taaccctacc tccgaactcg accgcgacga tctacgtgcc
    19741 ggcacggagt gggaacgacg tgacggagtc gggcgcttcc gccgcaaaag cgcaaggtct
    19801 gaggttctcc cgcatggaaa acggcaacgc ggttttcgaa gcagccagcg gacgctatcg
    19861 cttccaaagc gtgacagcgc agtagtgtct gggcagtatg gggccgttac ggcttccggt
    19921 aactgaagct gagatgcgcc gtggatccgt atgatccctg cttctccgtc aaggcccggc
    19981 gtaaacactg ctgggcgaag gagcattcgt ccgagaggtc ctggcgtccg gtcagctcga
    20041 ctttatattc ggcactgcaa gtggtgcaga caaacctgag ccattgcttg gtagcaatcg
    20101 gaagactcag gaggcgccgt acccgttcct ggatcttcag ttcgacgtgg ccgccacagt
    20161 ggccgtggaa catttcgaaa cccgattcct ggccggcgag cagaaatgga ttgaaccgct
    20221 cctgcttctg aaaatcgcgt ttcggggcaa cctggatttc catcggcggg gcgagtttat
    20281 gcagagggat cggctctgcg agtcgcttgc gcatggcata ctcctgtggg taagcaatag
    20341 tgtacagggg gatttgccga aaggaaattc cagtacttcc cggtacgaat cagagttagt
    20401 taacaggtgc caaccgacac atccagtcaa ggtgccgata tccttagggg ccgctcgaga
    20461 gaactcttcc agcccaccac acgtcc
//
LOCUS       DATIFQ010000353         4492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_115671, whole genome shotgun sequence.
ACCESSION   DATIFQ010000353 DATIFQ010000000
VERSION     DATIFQ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115671"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VLE22_16885"
     CDS             complement(<1..218)
                     /locus_tag="VLE22_16885"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HSB16132.1"
                     /translation="MKAMNPNQVGEQPKQPIPGVSHLVAVGSGKGGVGKTTVAVNLAV
                     ALARLGHKVGLLDADVYGPNVPLMMGIR"
     gene            complement(262..2289)
                     /locus_tag="VLE22_16890"
     CDS             complement(262..2289)
                     /locus_tag="VLE22_16890"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HSB16133.1"
                     /translation="MPGRRVALSCLLACLSLSASVVKVELVDRDDVLDGRSMGPAGPY
                     ERIVAKVYFAVDPKLPANRIISDIDLAPENAQGKVEFSADLYLMKPTDPAMGNGTVLF
                     EVSNRGGRGLVSTFNRALPVDDPSAKGEFGDGFLFEQGFTLAWLGWQHDIPSDPKLLR
                     LYAPVARDAGRPITGLVRSEYVPNRRMRSFSLADRTMIAYPVIDPRDPSIQLTVRDSV
                     EGKRRIVPKSQWRFAREENGTPVSDRAHVFVGAGLEPGKIYEIVYKAQDPVLVGLGPA
                     GIRDFISFLKHGAPGSGATLIGDERRHIKRAIGFGTSQSGRFLRTFLYYGFNQDEQHR
                     RVFDGVWAHVAGAGRGSFNHRFAQPSRDGQTYMNFFYPTDIYPFSDLDQADPETGLDE
                     GLLTRATRAHVVPRVFYTNSSHEYWGRAASLIHTTLDGAADFAPPKDTRIYLFAGGQH
                     GPSAFPPQRDGRRYLGNPNDFRWSMRALLVAFQRWLADGVEPPHSRYPTIAAGALVAP
                     RAVRFPKIPGIQTSTRVHKAWRMDYGPEFRTKGIVSIEPPRVGKAFPVLVPQVDSDGN
                     ETSGIRMPEVQAPLATYTGWNLRDPRIGAPQELFGLAGSWIPFPSTAADHKKTRDPRP
                     SIQERYAGRADYLKKIEAAARGLARDGYLLDRDVPGVVSIAGKHWDYLMKH"
     gene            complement(2313..3392)
                     /gene="tsaD"
                     /locus_tag="VLE22_16895"
     CDS             complement(2313..3392)
                     /gene="tsaD"
                     /locus_tag="VLE22_16895"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HSB16134.1"
                     /translation="MRILGIETSCDETAAAVVEDGTAIRSSVVSSQLDTHGKYGGVVP
                     ELASREHLRGIVPVVRLALEQASTDYDGLSAVAVTSGPGLVGALLVGMTYAKAICFVR
                     GLPLIGVNHIEGHIHSVILEARQSGNPVEYPALALVVSGGHTHLFEVLEGFHYRLLGK
                     TRDDAAGEAFDKVAKLLGFTYPGGPVIDSLAPYGNPRAVPFTLARMKGNPLDFSFSGL
                     KTAVLRWTEQRDLSAELAARRELVRRNPSPSVEEWIAVTSKATLDVLSSFQRTVIEEL
                     LRRAVASAGNIAARSLIISGGVACNSGLRRAASQLRLPYGVHFPTPGLSTDNAAMIAA
                     AAFPKYERGEFNDLSLAARANLTLA"
     gene            3443..>4492
                     /gene="queA"
                     /locus_tag="VLE22_16900"
     CDS             3443..>4492
                     /gene="queA"
                     /locus_tag="VLE22_16900"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HSB16135.1"
                     /translation="MNLSEFDYHLPEGRIAQQPLEDRAASRMLVIYRADGRWEDRTFR
                     ELPSFLEPGDCLVLNDSKVFPSRLFGRRSGARAMPIGKKNPKRWQYLTGQVEVFLLRP
                     VGGDSLTWLALVHPGRKMHVGERVRFSDALEAEVLARGAYGERTVRFHCEGDIYDRLN
                     ETGHVPLPPYIKRPDSPEDRERYQTVFARETGSVAAPTAGLHFTPEVLEQCRSAGASV
                     AFVTLHVGLGTFQPIHAEVVEQHRLHPESFHIREEDARTMKQARRLVAAGTTAVRTIE
                     SAARRGGLASCGGETDLYIYPGFEFQATGAMLTNFHLPRSSLLLLVCAFAGKDLVLSA
                     YRHAVEAGYRFYSYGD"
BASE COUNT          850 a         1330 c         1493 g          819 t
ORIGIN      
        1 tcccggattc ccatcatgag cgggacattc ggaccgtaga catcggcgtc cagaaggccg
       61 accttgtgac caagccgcgc caaggcgacg gccaggttga ccgccacggt cgttttgccg
      121 acgccgccct tgcccgatcc gaccgcgacc aggtgcgaca caccgggaat gggctgtttg
      181 ggctgttcgc ccacctgatt gggattcatt gctttcatcg tagcatcgga caagttcgtt
      241 cccgatgcgc gatcccgttg gctagtgctt catcaggtaa tcccagtgct tgccggcgat
      301 tgagacaact ccaggcacat cacgatcgag gagatagccg tcccgagcga gcccgcgggc
      361 agccgcttcg atcttcttca ggtagtcggc acgaccggcg tatcgttcct gaatcgatgg
      421 gcgcggatcg cgggttttct tgtggtcggc ggccgtcgac gggaaaggaa tccacgagcc
      481 ggcaagaccg aacagctcct gaggcgcacc gatcctcggg tctctaagat tccaaccggt
      541 gtacgtcgcg agtggagcct gcacttcggg cattctgatc ccgctcgttt cgttaccgtc
      601 cgagtccact tgcgggacca gaaccggaaa cgcttttccg acgcgcgggg gctctatgga
      661 cacgatccct ttggtgcgga actcgggccc gtagtccatt cgccaggcct tgtgaacgcg
      721 ggtcgaggtc tgtattccag gaatctttgg aaaacgtacg gcccgtggtg cgacgagcgc
      781 accagcggca atggtcgggt atcgcgagtg aggcggttcg acaccatcag caagccagcg
      841 ctggaaggcc accaacagcg cacgcatcga ccagcgaaag tcgttcggat tgccgaggta
      901 ccgcctgcca tctcgctgcg gcgggaaggc ggatggaccg tgctggccgc cggcgaagag
      961 gtagatcctc gtgtccttcg gcggcgcaaa atcggcggcg ccgtcgaggg tcgtgtggat
     1021 gagagacgcg gcgcggcccc aatattcgtg tgagctgttt gtgtagaaga ctctggggac
     1081 aacatgcgcc cgcgtggcgc gcgtgagcag gccctcatcc aggccggttt ccgggtcagc
     1141 ctgatcgagg tcggaaaacg ggtagatgtc cgtggggtag aagaagttca tgtacgtctg
     1201 gccgtcgcgc gacggctggg cgaagcggtg attgaaactg ccgcgtccgg ccccggcgac
     1261 atgcgcccat acgccgtcaa agacgcgccg gtgctgctcg tcctggttga agccgtaata
     1321 gagaaacgtg cgcagaaaac gaccgctctg cgaagtccca aacccgatcg cccgcttgat
     1381 gtggcgcctc tcgtcgccaa tgagcgtggc cccgcttccg ggagcgccgt gcttgagaaa
     1441 ggaaatgaag tcgcggatgc cggcgggccc gagtcccacc aggaccgggt cttgcgcttt
     1501 gtagacaatc tcgtagatct taccgggctc gagtcccgcg ccaacgaaga cgtgtgcccg
     1561 gtcggacacc ggggttccat tctcctcgcg ggcgaatcgc cactgactct tcggcacgat
     1621 ccgccgtttt ccttccacgg aatcccggac cgtaagttgg atggaagggt cacgcgggtc
     1681 gatgaccgga tacgcgatca tcgtccggtc cgcaagcgag aaactacgca tacgccgatt
     1741 tggcacgtac tccgagcgaa cgagtccggt gatgggtcgt cctgcgtcac gagccaccgg
     1801 agcatagaga cgcaggagtt tcgggtcgct cggaatgtcg tgttgccatc cgagccaggc
     1861 cagtgtgaaa ccctgctcga aaagaaagcc gtcaccgaac tctcctttgg cgctgggatc
     1921 gtcgacaggt aaggcccggt tgaaggtgct gacgagaccg cgtccgccac ggttggagac
     1981 ttcgaacagg accgttccgt tgcccattgc cgggtcggtc ggcttcatca ggtagagatc
     2041 cgccgagaac tcaactttgc cttgagcgtt ttcgggcgcg aggtcgatgt ccgagatgat
     2101 gcggttggcg ggcagtttcg ggtcgacggc gaagtagacc ttcgcgacga tccgttcgta
     2161 tggaccggcg ggccccatcg acctgccgtc gagcacgtcg tcacgatcaa cgagttccac
     2221 cttcacaacc gaggcggaaa gagagagaca ggctagcaga caagaaaggg cgacgcgtcg
     2281 tcccggcata gagaaatatt agacggtgag gctcaagcga gagtcaaatt ggcccgagct
     2341 gccagggaga ggtcgttgaa ttcgccgcgt tcgtatttcg ggaacgcagc ggctgcgatc
     2401 atggcggcgt tgtcggtgga aagacccggc gttgggaaat ggacgccata cggcagccgg
     2461 agctgcgagg cggctcggcg aaggccggag ttgcaggcga caccgcctga gatgatcaag
     2521 gaccgggcgg cgatgttccc ggcgctggcg accgcacggc gcaggagttc ctcgatgacc
     2581 gtgcgctgga aggaggagag cacgtcgagc gtggccttgg aggtaaccgc aatccattcc
     2641 tcaacggacg ggcttgggtt acggcgaacg agttcgcgac gtgccgcaag ttccgccgag
     2701 agatcacgct gttcggtcca gcgcaggacg gctgtcttga ggccgctgaa gctgaaatcg
     2761 agtggattgc ccttcatgcg ggcgagagtg aaggggactg cccttggatt gccgtacggc
     2821 gcgagactgt cgatgacggg gccgcctggg tacgtgaagc cgagcagttt cgctaccttg
     2881 tcgaacgcct ctccggcggc gtcgtcacgc gtcttaccga gcaggcggta gtggaatccc
     2941 tcaagaactt caaacagatg cgtatggccg ccggagacga cgagggcgag cgccgggtat
     3001 tcaaccggat tgccagactg ccgggcctcc agaatcaccg agtgtatgtg tccctcaatg
     3061 tgattgacgc cgatcagggg caggccgcga acaaagcaga tcgccttggc gtaggtcatg
     3121 cccaccagca gcgcgccgac gagcccgggg ccggaggtca cggcgacggc ggagagtcca
     3181 tcgtagtccg tggaagcctg ctcaagagcg agcctcacga cgggtacgat tccgcggaga
     3241 tgctcgcgcg aggcgagttc cggcactacg ccgccgtact tgccgtgagt gtcgagctgc
     3301 gaggagacga cggacgaccg tatggcggtt ccgtcttcga ccaccgccgc cgctgtctcg
     3361 tcgcaggacg tctcgatgcc aagaattcgc aagctgttat cctaacgggt ggaccgccga
     3421 gagcggccga tcgctcgcgg ccatgaacct ctctgaattc gattatcacc ttcctgaggg
     3481 gcggattgca cagcagccgt tggaagaccg ggcagcctcc aggatgctcg tcatctatcg
     3541 agcggacggg cggtgggagg atcgcacgtt ccgcgagttg cccagcttcc tcgaaccggg
     3601 cgactgtctc gttctgaacg attcgaaggt gttcccgtca cgactgttcg gccggcgttc
     3661 gggagcgcgc gcgatgccga tcgggaagaa gaacccgaag cgatggcagt acttgacggg
     3721 acaagtcgaa gtgttcctct tgcgtccggt gggaggcgac tcactgacct ggctggcgct
     3781 ggttcatccg gggcggaaaa tgcatgtcgg ggagcgtgtc cgcttcagcg atgcgttgga
     3841 agcggaggtc ctcgcgcggg gggcctacgg ggaacggacg gtgcgtttcc actgcgaagg
     3901 agacatctac gatcggttga acgagactgg ccacgttcct ttgcccccgt acatcaagcg
     3961 accggattcg ccggaggacc gcgagcgcta ccagacggtg ttcgcacgcg agacaggatc
     4021 ggtagccgcg ccgaccgccg gtctgcactt cacgccggag gtcctcgaac agtgccgtag
     4081 cgctggcgcc agtgtggcat tcgtcacgtt gcatgtcggt ctcggaacgt tccagccgat
     4141 tcacgccgag gtcgtggaac agcaccggct gcatccggag tccttccaca tccgtgagga
     4201 agacgcacgc acgatgaagc aggcgcgacg cctagtcgcc gccggcacga cggcggtgcg
     4261 gacgatcgaa agcgccgcaa ggagaggcgg gctggcgagt tgcggcggtg aaaccgacct
     4321 ctacatctac cccggcttcg aatttcaggc caccggcgcc atgctgacca actttcacct
     4381 cccacggagc agccttttgc tgcttgtgtg cgcgtttgcc ggaaaggatc tggtcctgtc
     4441 cgcttatcgc catgccgtgg aggccggata ccggttttac tcttacggcg ac
//
LOCUS       DATIFQ010000354         5815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_115926, whole genome shotgun sequence.
ACCESSION   DATIFQ010000354 DATIFQ010000000
VERSION     DATIFQ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115926"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VLE22_16905"
     CDS             complement(<1..809)
                     /locus_tag="VLE22_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16136.1"
                     /translation="MRKYGWPPFAAAFLFLLALPASAGLRAGAARRPVTPNLTKHAPV
                     YMEGFGQNRVATGIHDDLWARCLKLAADRTPRVLCGVDSIGLFLEDVERIRAKAHARL
                     GAADVVVAATTGLWGPKMGVSGINEEYNQFVVEQTAAAAVQAVRSLRPARVRTSKVST
                     PELDGFLNDTHPPVVHDSELVVLSATGRDGRAIGALVNWADHPETLGSKNTLVTADYP
                     AFFYPRMEELTGGVAVLINGAIGGMQSSLGAKVTDPKTGQPAPADSFRMAE"
     gene            complement(857..1816)
                     /locus_tag="VLE22_16910"
     CDS             complement(857..1816)
                     /locus_tag="VLE22_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB16137.1"
                     /translation="MRVGVFTALLANLSLDQVIEKLKDLGVTTVELGTGNYPGDPHCK
                     LAMLEDKTALKEFKQKLEDNGFTISALSCHGEPLHPNRAIAKAHQEVSKKTILLAEKL
                     GIPVVIDFSGCPGDSDKAKYPNWVTAAWPPHFPELLAWQWEKKVIPYWTKRANFAANH
                     GVKIALEMHPGFVVYSPETMLKLRAAAGKNIGANYDPSHMFWQGIDPIAAIRVLGDAI
                     FHVHAKDTQIYQRNLPTTGVLDTKTYTDERKRAWIFRTVGYGHGAEWWSEFISTLRMY
                     GYDYVLSIEHEDSLMSAEEGLTKAIRFLSDIVIREKPGVAYWA"
     gene            1910..3544
                     /locus_tag="VLE22_16915"
     CDS             1910..3544
                     /locus_tag="VLE22_16915"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HSB16138.1"
                     /translation="MNTVFLFLAGAALMAPPEVALRVAERMMLPCDIEKLGVVERPAV
                     SMAAGEAEPIALLVTTTAALENEPLTIRGLPRGVRAEVRIASPYRRRLARNREATQPF
                     LLEAVDSVTMREPGKSVYWILLRADETARAGHYAVSFRLRDATARVSVTVRPFRLRRD
                     HGVFYGAFCGARDRDITPAHMRDLRERGFDALQFFWGSLTVPLSNDAGTLAVDFSFVD
                     RWMAEFTKAGMKGPVVWSLGNDSVSHLENRLSDLFNIPRPEPVVVDGKRKNFSDVNNP
                     ELNRRLKELMLAIKRHAAEQKWPELVFLIYDEPTERLMEEHENRYKFIKSFWPELRIY
                     GVTMDRIAWAKAINHMVDILVANGDFDEIRGLAEQSGKPFWMYGSASGRDEASLRQAY
                     AWRPWLHKGNAVWFWAYNYHPGDPFDDFDGGSADSGMSMVWPPRTADSPFPYSVSWEG
                     MREAADDMAYVKTLEWMLEQTSSPRAREIRAGLDGIKAKIPSGRRVRVAGGDAHDTVE
                     RVDAGRFVTESRDAVAGWIEELLKLEKGRFREIRVR"
     gene            3620..4975
                     /locus_tag="VLE22_16920"
     CDS             3620..4975
                     /locus_tag="VLE22_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB16139.1"
                     /translation="MTTTLQNLTGRRGFMRSAAGALAASFWADETLEAATQRVNTSSK
                     PSDLKITDLRVAVVAKAPMTCPLIRIDTNQGIYGLGEVRDGASKTYALILKNRLLGEN
                     PCNVDRIFRKIKQFGFHARQGGGVCGVEMALWDLAGKAYGVPVYQMLGGKFRDTIRCY
                     ADTTESHDPKVYGERLKKRRDQGFTWLKMDLGIDLVEDIPGAVTHPAGVDSKTARLTE
                     HMLTGIELTEKGIALMADYVGQIREVVGWDIPLCADHFGHIGVNSCIRLGRALEKYNL
                     AWLEDMIPWQHTELWKKITDAVDIPTLTGEDIYLKEPFIELCRNHAVDIIHPDLATSG
                     GILETKKIGDAAQEYGVPMAMHFAGTPVSCMANVHCAAATENFLVQENHSVDVPWWDD
                     MVEGIEKPIVNKGFIKVPEKPGLGITLNDEVVKQHLLEPGYFEPTPEWDKERSHDRLW
                     S"
     gene            4982..>5815
                     /locus_tag="VLE22_16925"
     CDS             4982..>5815
                     /locus_tag="VLE22_16925"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB16140.1"
                     /translation="MNRRSFLAALSAPVLSPVTAPGRVPQNLRMTDVQVVVTNPNRAS
                     AGNYVLVKVITSEPGLYGWGDATCTGSELGIAKFLEEHLKPAMIGRNPMRIEDIWQTL
                     FLLPYYRSGSVQMSAISGVDMALWDIKGKVAALPVYELLGGRAREKLLTYTSVGGRNF
                     QEVEDGVRRLMEKGYKVIKVQVAPPGAESGYAVPSSERQQAATKKAFDGGVPPEETWE
                     PGPYVRTLPKLFEHLRKTVGEEIELLHDVHERVTPSQAIVLAKAVERYNLFYFEDPLR
                     PE"
BASE COUNT         1140 a         1709 c         1894 g         1072 t
ORIGIN      
        1 atctctgcca tccggaagct gtcggctgga gccggttgac cggtcttggg atcggtgacc
       61 tttgcgccga gagaggattg catcccaccg atcgcgccgt tgatcaacac cgccacgccg
      121 ccggtcaatt cttccatccg gggatagaag aacgccggat agtccgcggt aaccagcgtg
      181 tttttcgagc cgagcgtttc ggggtggtcg gcccagttga ccaaggcgcc gatggcgcgg
      241 ccgtcccttc cggtcgcgga gagaactaca agctcggagt cgtggacaac gggcgggtga
      301 gtgtcgttga gaaagccgtc aagctcgggc gtcgagactt tcgaggttct cacgcgggcc
      361 gggcggagcg atcgaacggc ttgtaccgcc gcagcggcgg tttgctccac cacgaactgg
      421 ttgtactcct cgttgattcc gctgacaccc atcttcggac cccagagccc cgtcgtcgca
      481 gccacgacga catcggcggc gccaaggcgc gcatgggctt tggcgcggat tctttcgacg
      541 tcctcgagga acaaaccgat tgagtcgaca ccgcagagga ctcgcggagt gcggtcggcc
      601 gccagcttca ggcagcgcgc ccagagatcg tcatggatac ccgtggccac acgattctga
      661 ccaaagccct ccatatagac gggcgcgtgc ttcgtgaggt tgggggtaac agggcgtctt
      721 gcggcgccgg cccgaagccc ggcggacgcc ggaagcgcga ggagaaagag aaaggccgcc
      781 gcgaacggcg gccatccata cttccgcatg agaatctctg cgggggcggg ttgcacgcac
      841 cgcccggtct cacttgctaa gcccaatacg cgacgccggg cttctcccga atgacgatgt
      901 cgctgaggaa tcggatcgcc ttagttagac cttcctcggc cgacatgagg ctgtcctcgt
      961 gctcgatcga gagaacgtag tcgtagccgt acatgcgaag cgtggagatg aactcgctcc
     1021 accactcggc gccgtggccg taaccgacgg tgcggaagat ccaggcgcgc tttcgctcgt
     1081 cggtgtaggt tttggtgtcc aggacaccgg tggtgggcag attccgctgg taaatctgtg
     1141 tgtccttggc gtggacgtgg aagatcgcat cgcccagcac gcgtatggcg gcgatgggat
     1201 cgatcccctg ccagaacatg tggctcgggt cgtagttggc gccgatattc ttgccggcgg
     1261 cagcccggag cttcagcatt gtttccggac tgtacacgac gaaacccgga tgcatttcca
     1321 gggcgatctt cacaccgtga ttggcggcga aatttgcgcg cttcgtccag taagggatca
     1381 ccttcttctc ccactgccag gcgagcaact cggggaagtg cgggggccat gcggccgtca
     1441 cccagttcgg gtacttcgcc ttgtcgctgt cgccggggca gccggaaaaa tcgatgacca
     1501 ccggaattcc gagcttttcc gccagcagaa ttgtcttctt gctcacttcc tggtgagcct
     1561 tcgcgattgc ccggttggga tgcagcggct cgccgtggca actcagcgcg ctgatagtga
     1621 agccattgtc ttccagcttc tgcttgaact ccttgagcgc agtcttgtcc tcgagcatcg
     1681 ccagcttaca gtgcgggtcg ccggggtagt tacccgtgcc caactccacg gtggtgacac
     1741 ccaggtcctt cagcttttcg atcacctggt cgagagacag gttcgccaga agtgcggtaa
     1801 agactccaac tctcacggca tctctccttt atttcttgaa gttgtgacag caatttgata
     1861 cgggaaaagg atagcacacc gactcaggta tgatcggtag ttcacgatca tgaacaccgt
     1921 atttttgttt ctggccgggg ctgcgctgat ggctccgcct gaggtggcgc tgagggtcgc
     1981 agaacgcatg atgcttccct gcgacatcga gaagctcggc gtggtcgagc ggccggcggt
     2041 ctcgatggcg gccggcgaag ccgaaccgat cgcattgctg gtcaccacga cggcggccct
     2101 tgagaacgag ccgctcacga ttcgcggctt gccgcgcgga gtccgcgccg aggtccggat
     2161 tgcgtcacct taccggcgga ggctggcgag gaaccgggaa gcgacgcagc ccttcctgct
     2221 cgaagcggtc gattccgtga ccatgcggga gccggggaaa tcggtgtact ggatcctgct
     2281 ccgcgcggac gaaaccgccc gggccgggca ctacgccgtc tcgtttcgcc ttcgcgacgc
     2341 cacggccagg gtgagcgtca ccgtgcgccc gttccgcctc cgccgcgatc acggcgtatt
     2401 ctatggagcc ttctgcggcg cgcgcgaccg cgacatcaca ccggcgcaca tgcgcgacct
     2461 gcgcgagcgc ggcttcgatg cactccagtt tttctgggga tcgctgacag tgcccctgag
     2521 caacgacgcc ggcacgctgg cggtcgactt ttcgttcgtt gaccgttgga tggcggagtt
     2581 cacgaaggcc ggaatgaagg gcccggtcgt gtggtcgctt ggaaacgact cggtcagcca
     2641 cctcgagaac cgtctgtcgg acctgttcaa cattccgagg ccggagcccg tggtggtaga
     2701 cggcaagcgg aagaatttct ccgatgtgaa taacccagag ctaaaccggc ggctgaaaga
     2761 gctgatgctc gcgatcaagc gccacgccgc ggaacagaag tggcccgaac tcgtgttctt
     2821 gatttacgac gagccgactg agcgcttgat ggaggagcac gagaaccggt acaaattcat
     2881 caaatcgttc tggccggagt tgcgcatcta tggtgtcact atggaccgca tcgcctgggc
     2941 gaaagcgatc aaccatatgg tcgacatcct cgtagccaac ggcgatttcg acgaaattcg
     3001 cggactggcc gagcagtcgg gtaaaccgtt ttggatgtac gggtccgcat caggccggga
     3061 tgaagcttct ctccggcagg cgtatgcatg gaggccgtgg ctgcacaagg gcaacgcggt
     3121 ctggttctgg gcatacaact atcaccccgg cgacccgttc gatgatttcg acggagggag
     3181 cgccgattca gggatgagca tggtatggcc gccgcgtacc gcggactctc cgttccctta
     3241 ttccgtgtcc tgggaaggca tgcgcgaggc cgctgacgat atggcttacg tgaagacact
     3301 agagtggatg ctggaacaaa caagcagccc cagagcgcgc gagatccgag ccggcctgga
     3361 tggaatcaag gcgaagattc cgagtggacg ccgggtacgg gtcgccggcg gtgacgcgca
     3421 tgacaccgtt gagcgggtgg atgccggccg gtttgtcacg gaatcgcgcg atgcagtggc
     3481 cggctggatc gaggaactgt tgaaactcga gaagggacgc ttcagggaga ttcgggtgcg
     3541 gtagcgcggg aaggcgcgcc ggtttgcggt cccgcgggcg ccacaataca atgagccaat
     3601 tcgaggagga atacctggta tgacaacaac ccttcagaat ctgaccggcc ggcgcggatt
     3661 catgcgctcg gccgcgggcg cgcttgccgc ctcgttctgg gctgacgaga cgctggaggc
     3721 cgccacccag agggtcaaca caagctcgaa accatccgat ctgaagatca ccgacctgcg
     3781 cgtggccgtt gtcgccaagg cgcccatgac gtgtccgctc atacgaatcg acaccaacca
     3841 gggcatctac ggacttggcg aagtccggga cggcgccagc aagacttacg ctctcatcct
     3901 caagaacagg ctccttggcg aaaacccctg caacgtcgac aggattttcc ggaagatcaa
     3961 acagttcggc ttccacgcgc gtcaaggcgg gggtgtgtgt ggagtcgaga tggcgctgtg
     4021 ggacttggcg ggaaaggcct acggagtccc cgtctaccag atgctgggcg gcaagttccg
     4081 cgacactatc cggtgttacg cggacaccac ggaatcacac gacccgaagg tttacggcga
     4141 gcggctgaag aagcgccggg atcagggttt cacatggctc aagatggatc tgggcatcga
     4201 tctcgtcgag gacattcccg gcgccgtcac ccatcccgcc ggtgtcgact ccaagaccgc
     4261 ccgtctcacg gagcacatgc tgacagggat cgagctgacc gagaaaggca tcgccctcat
     4321 ggcggattat gttggacaga tccgggaggt cgtcggctgg gacattccgc tctgcgcgga
     4381 tcacttcggg cacatcggcg tgaactcctg catccggctc ggcagggcgc ttgaaaaata
     4441 caatcttgcc tggctcgaag acatgatccc ctggcaacac acggagctgt ggaagaagat
     4501 caccgacgcg gtcgacatcc cgacgctcac cggtgaagac atctatctca aggagccgtt
     4561 catcgagctg tgccggaatc acgcggtgga tatcatccac ccggacctcg cgacgtcggg
     4621 cggaatcctc gagaccaaga agatcggcga tgcggctcag gaatacggcg tgccgatggc
     4681 gatgcacttc gccggaacgc cggtctcctg catggccaac gtgcattgcg cggccgcgac
     4741 ggagaatttc cttgtgcagg agaaccactc ggtggatgtg ccgtggtggg atgacatggt
     4801 ggagggtatc gagaagccta tcgtgaacaa gggcttcatc aaggtgccgg agaaaccggg
     4861 gctgggcatt acgctgaatg atgaagtcgt caagcagcac ctgctcgagc caggctattt
     4921 cgagccgacg ccggagtggg acaaggaacg atctcacgac cggctctgga gctaaccgct
     4981 catgaatcgt cgaagcttct tagccgctct ttcggccccg gtgctgtcac ccgtgacggc
     5041 gccgggccgc gtcccgcaga acctacggat gaccgacgta caggtggtgg tgaccaatcc
     5101 gaaccgggcg tcggcgggga actacgtgct ggtgaaggta atcacgagcg agccgggtct
     5161 atacggctgg ggcgacgcca cctgcacggg cagcgaactc ggcatcgcga aattcctcga
     5221 ggaacacctg aagccggcga tgatcggcag gaatccgatg cgaatcgagg acatttggca
     5281 gacgttgttc ctgctgccct actatcgctc gggctccgtg cagatgtccg cgatcagcgg
     5341 cgtggacatg gcgctctggg acatcaaggg caaagtggct gcgctgccgg tatacgagct
     5401 tctcggcggc agggcgcggg agaagttgct aacgtacacg agcgtcggcg gccgcaactt
     5461 ccaggaagtg gaagacggcg tccggcggct gatggagaag ggctacaagg tcattaaggt
     5521 tcaggtggcg ccgcccggcg cggagtcggg ctacgccgtt ccgtcgagcg aacgccagca
     5581 ggcggcgacg aagaaagcct tcgacggggg agtgccaccg gaagagacct gggaaccggg
     5641 cccgtacgtt cggacacttc cgaagctctt cgagcacttg cggaagacag tgggcgagga
     5701 aatcgaactg ctgcacgacg tccacgagcg cgtgacgccg agccaggcga ttgtgcttgc
     5761 caaggcggtc gagcgatata acctgtttta cttcgaggac ccgctgcggc cggag
//
LOCUS       DATIFQ010000355         4939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1458225, whole genome shotgun sequence.
ACCESSION   DATIFQ010000355 DATIFQ010000000
VERSION     DATIFQ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458225"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VLE22_16930"
     CDS             complement(<1..624)
                     /locus_tag="VLE22_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16141.1"
                     /translation="MSTIRRREFAAGFITPVLAAAQHAETPARIRARKRFSEIAASLY
                     AWDLLDEGVDPALDTLGEAAGANSTYLVALMHWEKRPLTDFYYPHNPKRKTYFPEDSR
                     AYWQPHLENYRDTRIKPRTSGRDELKGTDWLEMLVTAGRKRKWKTGAEISHTVLDFER
                     ARGECSYAVQRDIYGNPLAQLICVNNPDARSYLIGLFTDLVKNYDIDF"
     gene            831..1523
                     /locus_tag="VLE22_16935"
     CDS             831..1523
                     /locus_tag="VLE22_16935"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSB16142.1"
                     /translation="MRVLLEQFAEAGCKEVAEQPVKWSKLGNIVCTLPGSGDGTILVG
                     AHFDTANSLGVVDNWSGVSLLPSLYQSLAGRERNHTFVFVGFTDEELGLRGSESYVKQ
                     MDAAQRSHMRAMVNIDCVGMTSTKVWARRADKRLLASLAQVAASLKLPLAGVNVGEIG
                     ESDSLSFASKKIPVIDIHSVTQENFQRLHEGRDTPDTIRMDDYYDTYKLVSIYLGFLD
                     ITLGRDSGETKR"
     gene            complement(1542..2249)
                     /locus_tag="VLE22_16940"
     CDS             complement(1542..2249)
                     /locus_tag="VLE22_16940"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB16143.1"
                     /translation="MIERSALIAAVMALIACPLPAADWISLFDGRTLDGWHVAAKPAD
                     RDRGFWKVAGGAITCDSRGRTDHDYVWLITDREFGDFELRLKVRGFRDSTGNSGVQVR
                     SRYDEEAGWLHGPQIDVHPPAPWRTGLIYDETRGVQRWIFPSLKNWEIDESHAPKGWR
                     WKADGWNELRIVCRGTRITTRLNGVLAADLNGEGVLDDEAHRKHNVGLKGCLALQLHS
                     KDDLYIQYKDLRLRPIE"
     gene            complement(2254..3036)
                     /locus_tag="VLE22_16945"
     CDS             complement(2254..3036)
                     /locus_tag="VLE22_16945"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HSB16144.1"
                     /translation="MPAAAWGWEITAEELSAWILDRTPEWLVVNKPGYVVCHPSKHGP
                     WSSLIGACRQYLGVARLHMPFRLDRETSGVVVLATDPKIGKRMQQAVQNRRVRKRYLA
                     ILHGELRKPFEAEGPIGQHPDSQIAMRRAVTTGGQDARTRFIPLAQGGGYTLVRVEPA
                     TGRLHQIRVHAAHIGHAVAGDKVYGPDEALYLEFVKRGFTERLSAELPFRRQALHCFE
                     AAFLPGRLWPEGLCFRAPVADDMVRFCREAMGIRWEPGWEED"
     gene            3116..4303
                     /locus_tag="VLE22_16950"
     CDS             3116..4303
                     /locus_tag="VLE22_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16145.1"
                     /translation="MSAHKPAIQRPVVMASMVALLVVSGCGRRDRTEAPVPPPSYNDT
                     ARFLAGLRGEPNGPYRRLERDAAWKDHAARLDRSWTRFQKAFLAPARTFETKELSGDV
                     PAGGFVFYPFSGPDIVYPLTFFPRGDTYVLVGLEPAGEQPIPSDYSRANLASALRTWR
                     VAAGSLLYRSFFITDEMEHQLRGKDAEGALPLILLLMARTGNRILGARHARLDARGWL
                     STEKDPKRAQVLEIVFRRPRDGRIVRLYYFSRDLARGMNPDSGFPQFLERQGPARTFV
                     KSGSYLLHSPRFRRLRRYVLENSIVLLQDDTGIPFRMFDERGWQVRLYGDYSPPGDPF
                     RHRVQEDLAAAFDDPERIVKRLGFSLGYGAGRRTSSLLFAVRNRPGPLLGHIAPVLPE
                     PQP"
     gene            4579..>4939
                     /locus_tag="VLE22_16955"
     CDS             4579..>4939
                     /locus_tag="VLE22_16955"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HSB16146.1"
                     /translation="MRRTRSSDPKKRRRDASRAKAVLPSTYDPAEREVASFPIVGIGA
                     SAGGFEALTQMLQNLPSDTGMAFVFVQHMDPEHKSMLPKLLSKATQMPVVEAQQGMVV
                     QPNHVYVIPPKSDLGVIN"
BASE COUNT          876 a         1632 c         1491 g          940 t
ORIGIN      
        1 aaagtcgatg tcgtagttct tcaccagatc cgtgaacaac ccgatcaggt agctccgtgc
       61 gtccggattg ttgacgcaaa tcaactgtgc cagcggattg ccgtagatgt cgcgctgcac
      121 ggcgtacgag cactcgcccc gcgcacgctc gaagtcgagc accgtatgcg agatctcggc
      181 gcctgtcttc cacttgcgct tccgccccgc ggtgacgagc atctccagcc agtccgtgcc
      241 tttcagctcg tcgcggccgc tggtccgtgg cttgatccgt gtgtcgcggt agttttccag
      301 gtgcggctgc caataggctc ggctgtcctc gggaaagtac gtctttcgct tcgggttgtg
      361 ggggtagtaa aagtccgtaa gcggtcgttt ttcccagtgc atcagggcga ctaggtaggt
      421 actgttggcg cctgcggcct cgccgagcgt atcaagcgcc ggatcgaccc cttcatcgag
      481 caaatcccat gcgtacaggc ttgccgcaat ctcggagaag cgcttccggg cccggatcct
      541 tgcgggtgtc tctgcgtgtt gcgccgccgc cagcacgggc gtgatgaatc cagccgcaaa
      601 ctcccgtctt cgaatagtcg acatggggac attatgcacc gtgaactcct tctatactgg
      661 gagcaaggca gaggccacca ttgattcggg tccggatatc tcttctcctg gctgcgtgcc
      721 tgctttcctc tgcacggttg gcactcagcc agcgggtttt cgtccagccg gtctcgcggg
      781 agattatcac ggaacgcctc cggttggcag cgtcgaagaa ctccgaacgg atgcgggtcc
      841 tcttggagca gttcgcggag gcgggctgta aggaggtcgc cgagcagcca gtgaaatggt
      901 cgaagctggg caatatcgtt tgcacgctgc ccggctcagg cgatgggacc attctggtcg
      961 gcgcacattt cgacacagcc aatagtctcg gcgtcgtgga caactggagc ggcgtgtcgc
     1021 tgcttccgag cctgtaccaa agtctcgccg ggagagagag aaaccatacc tttgtgtttg
     1081 tcggcttcac cgacgaagag ttggggctac gcggctccga gtcctacgtc aaacagatgg
     1141 acgcggcaca acggtcgcac atgcgcgcga tggtcaacat cgactgcgtc ggcatgacca
     1201 gcaccaaggt ctgggcgcgg cgcgccgaca agcggctgct tgcttcgctg gcgcaggtgg
     1261 ctgcatccct caagcttccc ctggcgggcg tgaacgtcgg cgagattggt gagtcggact
     1321 ccctgtcatt cgcttccaag aagatcccgg tgatcgacat ccattctgtg acgcaggaga
     1381 acttccaacg cctccacgaa ggccgcgaca cgccggacac gatccgcatg gacgactact
     1441 acgacaccta caagctggtt tccatctatc tgggattcct cgacatcacg ctgggccggg
     1501 actccggcga gacaaagcgg tagaactcct caccatcgcg gtcactcgat cggtcgcagc
     1561 cggagatcct tgtactggat atagaggtca tccttgctgt ggagttgcag agcgaggcaa
     1621 cccttcaagc cgacgttgtg cttccggtgg gcctcatcgt ccaggacgcc ctcaccgttc
     1681 aagtcggcgg cgagcacgcc gttgagtcgc gtggtgatgc gcgtcccccg gcacacgatc
     1741 cgtaactcgt tccagccgtc cgccttccac ctccagccct tcggcgcgtg cgactcgtcg
     1801 atctcccagt tcttgagcga cgggaagatc cagcgctgca cgccgcgtgt ctcgtcgtag
     1861 atcaacccgg tgcgccacgg cgccggcgga tggacgtcga tctgcggccc gtgcagccag
     1921 ccagcctcct catcgtaccg gctgcgcact tgcacaccgg agttgccggt ggaatcacga
     1981 aagccacgaa ccttcagtct cagctcgaaa tcgccgaact cgcgatcggt gatgagccag
     2041 acgtaatcgt ggtccgtgcg gccacgggag tcgcacgtga tcgcgccacc ggcgaccttc
     2101 cagaatcccc tgtcgcggtc ggcgggcttc gccgcaacat gccagccgtc gagcgtccgt
     2161 ccgtcgaaca gcgagatcca gtcggccgcg ggcagcgggc aggcgatcag agccatgacc
     2221 gcggcgatca gtgcagaacg ctcgatcacc ggttcaatcc tcctcccaac caggctccca
     2281 tcttatcccc atcgcctcac ggcagaagcg gaccatgtcg tctgccaccg gcgcgcggaa
     2341 acacagcccc tccggccaca accgccccgg aaggaacgcc gcctcgaagc agtgcagtgc
     2401 ctgccggcgg aatggcaatt ccgccgacag ccgctcggtg aagccccgct tcacaaactc
     2461 caaatacagt gcctcgtccg gtccatagac cttgtcgccg gcgacggcgt ggccgatgtg
     2521 cgccgcgtga acacgaatct ggtgcagccg ccctgtggcc ggctccacgc gcaccagcgt
     2581 gtagcccccg ccctgtgcca gggggatgaa ccgcgtgcgc gcatcctgcc ctcccgtcgt
     2641 caccgcccgc cgcatcgcga tctgcgaatc ggggtgctgg ccgatcggcc cctccgcttc
     2701 gaagggctta cggagctcgc cgtgcaggat cgccaggtac ctcttccgca cccgccggtt
     2761 ctggaccgcc tgttgcatcc gttttccgat cttcggatcg gtcgcgagca ccacgacgcc
     2821 gcttgtctcg cggtccagcc ggaacggcat gtgcagccgg gcgacgccga gatattggcg
     2881 gcaggcgccg atcaggcttg accacggccc gtgttttgag ggatgacaga cgacataccc
     2941 cggcttgttg accacgagcc actcgggcgt gcggtcgaga atccacgcgg acagctcctc
     3001 ggccgtgatc tcccatcccc acgcggcggc cggcatgtca gttcccgttt cccgcccgga
     3061 tgccgtgtaa cctttgtgcg cctgcaatca tcatctgtca gagtagagag tgactgtgtc
     3121 cgcacacaag ccagcgattc aacgaccagt cgtcatggcg tcaatggtgg cgctgctggt
     3181 cgtctccggt tgcggccggc gcgacagaac cgaagcgcct gtcccgccgc cctcttataa
     3241 cgacacggcg cgtttccttg ccgggctgcg gggcgaaccg aacggcccgt atcgccgtct
     3301 ggagcgcgac gcagcgtgga aagaccacgc ggcacggctt gacagaagct ggacacgctt
     3361 ccagaaggcg ttcctcgcgc ccgcccggac cttcgagaca aaagagctct ccggggacgt
     3421 tcccgccggc ggcttcgtgt tttatccgtt cagcggaccg gacatcgtgt atccgctcac
     3481 cttcttcccg cgcggcgaca cctacgttct ggtcggactc gagccggccg gggaacagcc
     3541 catcccctcg gactactcgc gcgccaacct tgcctccgcc ttacgcacct ggcgtgtcgc
     3601 cgccggctcg ctgctctacc ggagtttctt cataactgac gagatggagc accagctccg
     3661 tggcaaggac gccgagggcg ctctgcccct cattttgctg ctgatggctc gcacaggaaa
     3721 caggatactt ggggcgcggc acgcccggct cgacgcaagg ggctggctct ccacggagaa
     3781 ggatcctaag cgcgcccaag tcctcgagat cgttttccgt cgtcctcgcg acggccgcat
     3841 tgtcaggctg tactacttct cccgtgatct tgcccgcgga atgaaccctg actccggttt
     3901 cccgcagttc ctggagcgtc agggccccgc ccggacgttt gtgaaatcgg gatcgtacct
     3961 tctgcacagc ccgcgcttcc gccgattgcg ccgttacgtg cttgagaaca gtatcgtcct
     4021 tcttcaagac gacactggca tccccttccg gatgttcgac gaacgcggct ggcaggtccg
     4081 gttatacggt gattacagcc cgcccggcga tcccttccgc caccgggttc aggaagacct
     4141 cgcggcggcg tttgatgacc cggagcgcat tgtcaaaaga ctcggtttct ctctcggcta
     4201 tggcgcggga cgccggacat cgagcctgct gttcgcggtt cgtaaccggc ccggcccgct
     4261 gctgggccac atcgcgccgg tgctgcccga accccagccc tagcgtgtta gcagggcggc
     4321 accactactt gcgacggcag tgcgcgccta tccgaagaga cagggggcct gacgtgattg
     4381 gtacgaagtg gaacgagacg ccgggaggat ctgaagcatc acgggatagg aaagctggcg
     4441 tttcacacat tcctacgcga ctcgggtggg acaacctgcg cgtgtaccac gtcctgaagt
     4501 agggagtcgt gcgacgtccc tgcaggggct tcaggttgtc agcggtgcat aatgatcttg
     4561 acggcagagg agttgcccat gcgaagaact cgctcatcgg acccgaaaaa gaggcggcgc
     4621 gacgcgtcgc gcgcaaaggc cgtccttccg agtacttacg accccgctga gagggaagtg
     4681 gcctccttcc ccatcgtcgg catcggggcc tccgctggag gcttcgaggc cttaacgcag
     4741 atgctgcaaa atctaccctc ggacaccggg atggccttcg tgttcgttca gcacatggac
     4801 cccgaacaca agagcatgct gccgaaattg ctctcaaagg ccacccagat gcccgtggtc
     4861 gaggcgcagc agggcatggt cgtgcaaccc aaccatgtct acgtaattcc accgaagtcc
     4921 gacctgggtg tcatcaatg
//
LOCUS       DATIFQ010000356         3639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_884612, whole genome shotgun sequence.
ACCESSION   DATIFQ010000356 DATIFQ010000000
VERSION     DATIFQ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884612"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2541
                     /locus_tag="VLE22_16960"
     CDS             <1..2541
                     /locus_tag="VLE22_16960"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB16147.1"
                     /translation="QALGMSNRRLPSGTFASMNREFQLETGEFLRTADDVRNVVVGVA
                     GGKPVFVRDVAEVTDGGEEPSQYVRYASAGGFHPAVTVSVAKRKGTNAIEVAENVLHR
                     VEALKGSVIPSDVRMTVTRHYGETSAEKSNELLFHMLIAVISVSLLIWLTLGFRESLI
                     VFIAIPVTLALTLTVFYLYGYTLNRITLFALIFSIGILVDDAIVVVENIVRHARLPGN
                     VGRPLGEVAIEAVDEVGNPTILATLTVIAAILPMAFVGGLMGPYMRPIPVGASAAMVF
                     SLIVAFLVTPWAAVRLLKVHGAHGNGEEEDRLTRLYRRVMNPLLHNFTLRWGFLAMVT
                     LLLLGSCALVYIKFVEVKMLPFDNKSEFQVIVDMPEGTTLEETARVTKKLADAVLTQR
                     EVVNLQTYVGTASPYNFNGLVRHYYLRRGSNVADIQVNLLPKQDRDEQSHQIAKRVRN
                     VILPIAKQYGANVKVAEVPPGPPVLQTLVAEVYGPAEEGRLRVARQIRGILEGTPGIV
                     DVDWYVEDDQPKYRLIVDKEKAALNGVSEEEIARTMRIASAGEPAGLLHADSEKEDVP
                     ISLRLDRATRSDLDHIQNLKVMGRTGSLVPLREVLRAEQTLAEKSIYHKNLMPVTYVT
                     ADVAGAMESPVYAILATSPKIAEIKIPEGYSIEQHTARQPFEDSRYSMKWDGEWHITY
                     EVFRDLGIAFAAVLILIYVLVVGWFQSFITPLVIMAAIPFSLVGILPAHGLLNAFFSA
                     TSMIGFIAGAGIVVRNSIILVDFIELRLKEGMPLDEAVVDAGAVRFRPMVLTAAAVVV
                     GAGVILFDPIFQGLAIALMAGEVASLALSRMTVPIVYYIVNKRKMAGS"
     gene            complement(2604..>3639)
                     /locus_tag="VLE22_16965"
     CDS             complement(2604..>3639)
                     /locus_tag="VLE22_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB16148.1"
                     /translation="VAPVTLDFLLRDASAIVVAGVEGNIVNGTAVGNLVIERALRGEL
                     RPGSAVQLTWTGRQTVQPMSRPLHKDRALFFLRKDAGGHWEIVPGTTGNVEFDMLYFL
                     LPSGLKPEEYRAGPGATIEEKVFLELAWAASVGWPPDRGAVYDLLDVFRGHLNSPAAR
                     KTFTQFSRSHVDKVQALDLRALIAEGDLGALERVASEQDKLMASPFGRAIAEDLRFYF
                     RNAEAAAVRILGPLATSENTRQEFRIAAATALARVHTRESLQHLAGLLESPDLMLRTL
                     AVGGLAMFANNVPVNSHHPAPGDWPYRTEDTIAHSTMDPNTIARNESYYVGFWKNWWF
                     QNKETLVTPP"
BASE COUNT          688 a         1120 c         1111 g          720 t
ORIGIN      
        1 caggcgctcg gaatgtccaa ccgccggctg ccgagtggga ccttcgccag catgaaccgc
       61 gagttccaac ttgaaaccgg cgagttcctc cgcacggccg acgacgtgcg gaacgtggtc
      121 gtcggtgtgg ccggcgggaa gcctgtcttc gtgcgcgacg tcgccgaggt gaccgacggc
      181 ggcgaagaac cctcgcaata cgtccgatac gcctcggcgg gcggtttcca ccctgccgtc
      241 actgtgtccg tcgccaagcg gaagggcacg aacgctatcg aggtggccga gaacgttctt
      301 catcgtgtcg aggcgctgaa gggtagcgtc attccgtcgg atgtacggat gacggttact
      361 cgccactatg gagagacttc ggcggaaaaa tcgaacgagc tgctgtttca catgctgatt
      421 gccgtcatct cggtcagcct gttgatctgg ctcacactcg gtttccgcga gtcgctgatt
      481 gtctttatcg ccatcccggt tacgctggct ctgacgctga cggttttcta tctctacgga
      541 tacacgctga accgaatcac gctgttcgcg ctcatctttt cgatcggcat cctagtcgat
      601 gatgccatcg tcgtggtcga gaacatcgtg cgccacgcgc gactgcccgg taacgtgggg
      661 cgcccgttgg gggaggttgc gatcgaggcg gtggacgaag tcggcaaccc gacgatcctt
      721 gccacgctga cggtaattgc cgcaattctg ccgatggcct tcgtcggagg actgatggga
      781 ccgtacatgc ggccgattcc ggtgggcgcc tcggcggcga tggtcttctc gctgatcgta
      841 gcgttcctcg tgacgccttg ggcggcagtc cggctgctca aagtccacgg agcccacgga
      901 aacggcgagg aggaggatcg cctcacgcgc ctgtatcgcc gggtgatgaa tccgctgctg
      961 cacaacttca ccctccgctg gggattcctg gcgatggtca ccctgctgtt gttgggctcc
     1021 tgcgcgctgg tgtacatcaa gttcgtcgaa gtgaagatgc tgcccttcga caacaaaagt
     1081 gagtttcagg tcattgtgga catgcccgag gggaccacac tggaggaaac cgcgcgcgtc
     1141 acgaagaagc tcgccgacgc agtactgacg cagcgtgagg tggtcaatct acagacttat
     1201 gtgggcaccg cgtctccgta caacttcaac ggtctggtcc gtcactacta cctgcgccgc
     1261 ggctcgaacg tcgccgacat tcaggtcaac ttgctgccga aacaggaccg cgacgagcag
     1321 agtcaccaaa tcgcaaagcg ggttcgaaac gtgattcttc cgattgcgaa gcagtacggt
     1381 gctaacgtca aggtcgccga ggtgccgccc ggtccgccgg tgctccagac gttggtcgcg
     1441 gaagtgtacg gaccagccga ggagggcagg ctccgggttg cccggcagat ccgcggcatc
     1501 ctcgagggca cacccgggat cgtcgatgtc gactggtacg tcgaggacga ccagccgaag
     1561 taccggctga tcgtggacaa ggaaaaggcg gcgctcaacg gcgtttcgga ggaggagatc
     1621 gcgcgcacca tgcgcatcgc ttcggcgggt gagccggccg gtctgctgca cgccgattcg
     1681 gagaaggagg atgtgccaat ctcgctgcgg ctcgaccgcg ccacacgctc cgacctcgac
     1741 cacatccaga acctgaaggt catgggtagg acgggcagcc tcgtgccgct gcgggaagtc
     1801 cttcgcgccg aacagacact cgcggagaag agcatctacc acaagaatct gatgccggtc
     1861 acctacgtga ccgccgacgt tgcgggcgcg atggagagcc ccgtctacgc catcctggcc
     1921 acgagcccca agatcgcgga aatcaagatc cctgaaggct actccatcga gcaacacacc
     1981 gcccggcagc cattcgagga ttcccgctac tccatgaaat gggatggcga gtggcacatc
     2041 acctacgagg tcttccggga cctcggcatc gctttcgccg cggttctgat cctgatctac
     2101 gttttggttg tgggttggtt ccagagcttc atcacgccac tggtgataat ggcggcgatt
     2161 ccgttctcgc tcgtgggaat cctgccggcc cacggtctgc tgaacgcgtt cttcagcgct
     2221 acgtcgatga taggcttcat cgccggcgcc gggatcgtcg tgcgaaactc catcatcctg
     2281 gtggatttca tcgagttgcg cctcaaggag ggaatgccgc tcgacgaggc cgttgtggac
     2341 gcgggcgccg tccggttccg gccgatggtg ctgaccgcgg cggcggtggt ggtcggcgcg
     2401 ggggtcatcc tgttcgaccc gatcttccag ggcctggcca tcgcgctgat ggccggcgaa
     2461 gtcgcgtcgc tggctctgtc ccggatgacc gtaccgatcg tgtactatat cgtgaacaag
     2521 aggaaaatgg cggggagctg aggcttcccg tcaccttttc cactgttccg catctgggtc
     2581 cgtaagagga gagtacgctc cagctatggc ggtgtgacga gcgtttcctt attctgaaac
     2641 caccagttct tccagaagcc gacgtagtaa gactcgttcc gcgcgatcgt gttgggatcc
     2701 atcgtcgaat gggcgatcgt atcctccgtg cggtaaggcc agtcccctgg agcagggtgg
     2761 tgcgaattga ccggcacgtt gttcgcaaac attgccagcc cgcctacggc caaggtgcgc
     2821 agcattagat cggggctttc tagcaatccg gcgaggtgct gtagagactc ccgggtatga
     2881 accctcgcca gcgccgtcgc cgcggcgatt ctgaattcct gacgagtatt ctcggaggtc
     2941 gccaatgggc cgagaatgcg cacggccgcc gcctctgcat tccgaaagta gaagcgcaag
     3001 tcctcggcaa tcgctctgcc aaaaggcgaa gccatcaact tgtcctgctc cgatgcgacc
     3061 cgctccaacg ctccaaggtc gccttctgca atcaaagccc tcaaatccag ggcttggacc
     3121 ttatcaacgt gactccggga aaactgcgtg aacgtcttgc gcgccgccgg ggaattgagg
     3181 tgccccctga aaacatccag gagatcgtag accgcgcctc gatccggagg ccacccgacc
     3241 gacgctgccc atgccagctc gagaaacacc ttctcttcga tcgttgcgcc cggtcctgcc
     3301 cggtattcct cgggcttcag cccgctcggc aacaggaaat acagcatgtc aaactctacg
     3361 ttcccggttg tccctggaac gatttcccag tgcccacccg cgtcctttcg gagaaaaaac
     3421 aacgccctgt ctttgtgaag gggtcgagac atcggttgaa ccgtctggcg tccagtccag
     3481 gtcagttgca ccgccgagcc gggtcgcaat tcaccccgca acgctcgctc gatgacgaga
     3541 ttgccgacgg ctgtcccgtt gacgatgttt ccctcgacac cggcgaccac gatcgcgctt
     3601 gcgtctctga gaagaaagtc caaagtaact ggcgcaacc
//
LOCUS       DATIFQ010000357        14158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1649692, whole genome shotgun sequence.
ACCESSION   DATIFQ010000357 DATIFQ010000000
VERSION     DATIFQ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649692"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VLE22_16970"
     CDS             complement(<1..268)
                     /locus_tag="VLE22_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16149.1"
                     /translation="MQAQITERNLPAVAEQYPVSVWAPPPLIEPDPEKPALPLSHYLW
                     ILKRQRWKIVAFVGMSVVGTLIVSARLTPVYESTATVDVDRQTPT"
     gene            complement(288..1226)
                     /locus_tag="VLE22_16975"
     CDS             complement(288..1226)
                     /locus_tag="VLE22_16975"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HSB16150.1"
                     /translation="MLPILFLLLCGALSAQVRSTSELGAANLPAQKIGPNDLIAISVY
                     DAPELTRTVRVGADGFIRLPMLKRRVRADGLLPAELEATVAEALHAEQLLVEPVVTVT
                     IAEYHSRPISVAGAVRNPVTFQAAGPMTLLDALTRAGGLSVDAGPEILVSRTQPGEDG
                     KPATLVQRIPVRGLIDAADPELNLKLAGGEEIRVPEVGKIFVVGSVKKPGVYPVQGES
                     ETTVLQMLALAEGLVPFAAKRAYIYRREAATGSKHEIAIELKQIMERKAPDIPLDAND
                     ILYIPDNSGRRVAVHTLEKIAGFGTATASGILIWRR"
     gene            complement(1324..1590)
                     /locus_tag="VLE22_16980"
     CDS             complement(1324..1590)
                     /locus_tag="VLE22_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16151.1"
                     /translation="MSVLRPRNRLVNFRLSQQEYETVKAVYAFTGARSISDFARTAVL
                     RSAGATEASHGPAQSPLSNLEHKVAELESRVTSLIRLLNDGAES"
     gene            complement(1624..2172)
                     /locus_tag="VLE22_16985"
     CDS             complement(1624..2172)
                     /locus_tag="VLE22_16985"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HSB16152.1"
                     /translation="MELFGNPLPNSYHSADWFALTVKPQHERAAAQALESKGLEFFLP
                     LYRARRRWSDRVKELDVPLFPGYVFSRFESGDRARVLATPSVRSVVGFGRTPAPVAEE
                     ELNAVRSIVASDLPSGPWPFLKVGQTVCIEAGPLSGLEGILVQLKDVCRVVVSVHLLQ
                     RSVAVEIDRDVLRPSGRPALAH"
     gene            complement(2258..3262)
                     /locus_tag="VLE22_16990"
     CDS             complement(2258..3262)
                     /locus_tag="VLE22_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16153.1"
                     /translation="MHRLTAACLLALFLYPVGIGAEAPASIGVAITKGEFRLDNHTVS
                     GNATIFDGNTLETFTDPSELRIQGGVRVLLDSGSRAKVFGGRLVLEQGHGEWDNNRGY
                     AVEALGLRIVPTGGASAGRIAYSGERSVQIAALRGGLRVMNTGGSVVAVMAPGMALEF
                     QPQAPAGAQLPFEMTGCVERRDGRFVLRDLIAGVLEEIRGDRLDREVGNVVEVVAFEL
                     PKEQPVAGAMEVVQVTRMRRVRRGCPAPPPSAAVPPAAKPAEPPEAARPKTAPPKAAP
                     QVAKTPAPPVTQPTPPVSAPSGGMSGAKKAVIAGVIVGGAGAGAAVYLTQKEKETISR
                     "
     gene            3706..4932
                     /locus_tag="VLE22_16995"
     CDS             3706..4932
                     /locus_tag="VLE22_16995"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB16154.1"
                     /translation="MAGNGSNGNGKNRRRRRRLIWLSVLAMLLVGAFFGVRAALRPNN
                     HIDPSKLAEVTRGDIARSVVATGKIEPRAKVEVKSKASGIVQRLLVDYGDWVKQGQVL
                     VELDKEELQARVREARATLLAAQAAEESSKATYERNKVEAEGPDIPFLKSGMDRAQKL
                     HTQGLIARSLLEDAEKAYQTALNKQMAALRNLSVARAEIARSQAQVAQARAALERADE
                     DLRNATVVSPMNGLVLSRNVETGDAVSSILVLGSQATLIMTLGDVSDVYVLGKVDQAD
                     IGKVYMGQRARIVVESFKDKKFEGQVTKIAPLGVEKENVTTFEVKVSIRNPSGELKAN
                     MSANAEIILEEKQNVLLIPESSIVYDRNRNTSIEVPDSTIEAGRRKVPVKLGISNGVK
                     TELISGLKPGEKVVLQ"
     gene            complement(4949..5485)
                     /locus_tag="VLE22_17000"
     CDS             complement(4949..5485)
                     /locus_tag="VLE22_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16155.1"
                     /translation="MKFRRSTGADDRLKRLANNIETLVRKDEERIREERKVAALRSQG
                     ARELHATCAAFVGAVNQLLRKPLVDLAPAEFTADTYRDAGGNLFQVNIAGRLIQIEFR
                     ATDTPISTEQYRVPYVLQGEVRCLNQESIERAVIPEHLLFYCLEKDSQTWLLFDPRTH
                     RTSDFDQECLISLLERLV"
     gene            5523..6152
                     /locus_tag="VLE22_17005"
     CDS             5523..6152
                     /locus_tag="VLE22_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16156.1"
                     /translation="MRIPYSALIASCICAAGLCAQDRPGLFFREDWKETPAEVPVGQA
                     HVVNPDLVLGLYGPGRTVIKKSHHDTPADDPYYVWSGLCPLNWAVTLKHKDAFADLSG
                     QAKLRWRSKQAGFRQLRIILKLAGGAWLVSDAYDGPSQDWRVREFNIQDIRWRKLDIE
                     KVVEGAWVERPDLSRVDEIGFTDLMVGGESIACSRLDWIEVDAKPVKRR"
     gene            complement(6179..8557)
                     /locus_tag="VLE22_17010"
     CDS             complement(6179..8557)
                     /locus_tag="VLE22_17010"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HSB16157.1"
                     /translation="MIRCIAPLFLAATLSEGSMVTLQQGVDGYEGSTTRTIVDTRPAA
                     EADDGYFHLRGSQNSLHARFELPKLSGTLARARLMIFLPSARKPNKFTEIFCHEAISG
                     GNQISYDELTDYGNGRRRGAVDSVELFAPPHEGWPDFPFLPLGVPEGGRWIEFNITPL
                     VEKWIAGEAANHGVLLIPADPPDRRTPSTWEIDVPTATYSSGDKLHPKLVLELDGDEV
                     VAGMTDGMTRICDLSTRFGYRGSYGTSYRMSMAANEFEGLQVVVQPMLRDIEGVRLNV
                     SDLVSAEGKTIPASDVSYFIEDVYPMRRNWMTRELMFGGKVYETTDPLLPTKPVRLRR
                     HVHTPFYVVVRTRPGTSPGVYRGKIAIADSARMLKDLELEVNVWPYAIPEKWNFHSMG
                     QFIWGNAARFHGEDPDAGLYRKYYDFLMEHRFSPTEQYAQILSPRKDLEELLRQGMNT
                     VYLSGNFAGTDTELAQLKKNYETVKRLGALDYTLVYIGDETDQWNEMRRRADLVHAHL
                     PGVQVMIGGSFPREELVGFVDIYDPQVSGRSKVYSLQEDQVNLIAESQKRGEEFYWYT
                     AAGPAYPYPNVQVENPLIASRVLFWTTWKYGVTGFEYYCYNIWERNYAKDPSKRYPNV
                     PWKADGWSHGWPTNGDGMLFYPGPISSLRFEAIRDGIEDWESHMVLRDAVEALRSRGT
                     VAAHTALIGKAEALLKVDAEIVSGFSKYTMDPARLLAERERLGGLIAELMPLAGGPPA
                     VERAASERVARQARLRRAMLWERHLRACEVLKVKPVPEPGWNALWPERPGEK"
     gene            complement(8654..9913)
                     /locus_tag="VLE22_17015"
     CDS             complement(8654..9913)
                     /locus_tag="VLE22_17015"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB16158.1"
                     /translation="MTWRWQISEAVSSLRFYRRRTLVTILSLAWGVASFVLLMSYGHG
                     FDQALLQGFQAVGQDLIIMAAGQTSLQAGGMRSGREVRLELSDVETLREAVPTVGAMS
                     PELLKYGTKVVRGTREKQYTLRAVWHEYQYIRNMQMVSGRWIGREDTLQRNRVAVLGA
                     KVAKEMFSGIPPVGEEIALQGQRFTVIGVLDAKGQLANYNTPDNNCIFIPYDTMSIYK
                     DLRYPDFIVWTPISPQVRDRAIRDVRATLGMIHRFSPNDEKAIFILAFNQFLYIIETM
                     SMAARMLLGFVGALTLGIGGVGLANIMLATVLDRTREIGVLKALGGPKGMILKQFLFE
                     ALIIVGIGGLLGVAVGAVATFALGSMPLFGAIMDDAPDKGNVELGLSLGSILVSTGVL
                     LFVGLVAGMIPAIKAARLDPIEALRYE"
     gene            complement(9918..11201)
                     /locus_tag="VLE22_17020"
     CDS             complement(9918..11201)
                     /locus_tag="VLE22_17020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB16159.1"
                     /translation="MSRISMRFLNVIYGFIEQTVANVWRHKLRSFLTMFGIAWGIASL
                     VLMSALCDGFRQGQRNNMKQLGESIVLVWGGRTERQAGGQRAGRHIRLWDKDVQVVRE
                     QCPLVSVVAGEVKREVAATSEFNSGLFLALGASPEYLQLRNLPVETGRTLNREDNQNA
                     RRVCVLGASVKEQLFGYRGDVLGRQVSIYGNPYHVVGLMSKKNQNSSYDGWDNDKILI
                     PKTSLERDCPPKWSDYQKGRIETLVYRPLSITEWEGAQRQVRTALGRLHDFDPLDESA
                     LPMWDTVKSAELFGAVFNATEIFLAVIAIITLSLGGVGVMNTMMTAVSERTHEIGLKK
                     ALGATKRRILLDFFLEGLLLAVLSGALGLAVVGALATAVNSLPMPAMFAGLPISYRNT
                     LLAVAALGTVALASALPPAWRASELTPVEALREER"
     gene            complement(11287..11652)
                     /locus_tag="VLE22_17025"
     CDS             complement(11287..11652)
                     /locus_tag="VLE22_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16160.1"
                     /translation="MFRLTGVFALMICTLLAAVPAFCATDLDGNWNFTFNSDDGDRQA
                     PATFKVDGGQVTGRFADRADVKGTYSERKFELKFHLVYEEGGLEGELKLTGKVDGGKV
                     TGNWEYAGYAGTFSATKVQ"
     gene            complement(11721..12497)
                     /locus_tag="VLE22_17030"
     CDS             complement(11721..12497)
                     /locus_tag="VLE22_17030"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB16161.1"
                     /translation="MTHTELFSLTDKVAVVVGGGGVLAGAMAEGLAGAGADVAIVDYN
                     KDGAEDRATSIRRLGRKALGIQADATQKPALEAACRQVLDELGSVDILINAAGINSAT
                     PFFEIQESEWQRILDVNLKSVFFACQVFGKVMVESGRGGSIINISSASSGPPLSKVFT
                     YSVTKGGVNQITQFLAREWAPYRVRVNAVLPGFFPAEQNRRILTPERTAQIMSHTPMN
                     RFGESEELVGATIYLASDKASSFVTGSIVRVDGGFLAMTI"
     gene            complement(12525..>14158)
                     /locus_tag="VLE22_17035"
     CDS             complement(12525..>14158)
                     /locus_tag="VLE22_17035"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSB16162.1"
                     /translation="APEGRSQTVRLEGSRVSLGRATSNELCFADDAGLSRQHLVFERD
                     GDEWVVADLGSKNGTEVNGERIPGPHRLQPGDRITAGHLVMVFDDPLGEMAESVVFVD
                     PTVADVPASTAMIANLDGVLGSEPVFVETASPSPFDLDIRVRALVHAGRELAGHRPLG
                     ELFPLILDLALDAVDADRGVLLTLEGERLLVRAARGDSFRISSAVRDRVVQQRESLLV
                     EDVQREDAFRSRKSIQAQNVRSMLAVPLQTSERVIGLIYVDSPNVVRQFSREDLNLLT
                     VMANVAAIRIEHARLADVEQVERILARELEQAAEIQRRLLPENPPPVSGLELAGHNAP
                     CRTVGGDYYDFFPQPDGRVGLAVADVSGKGMPAALLMSSLHAFMQVLAEDLSDLSRLT
                     NRLNRLLTARSLSNRFVSLFLGIVDPASGELAYCNAGHNPPLLVRACGNTEWLDGGGP
                     ILGVFPDTVYEQHTRLIAPGDTLVLYSDGVTEIASPSGEEYGEDRLAALMVERRGRPA
                     AAIVDDLRNALIEWTAGAAPADDVTLVVARRTSPI"
BASE COUNT         2675 a         4675 c         4183 g         2625 t
ORIGIN      
        1 ccgtcggcgt ctgccggtcc acgtccaccg tcgccgtcga ttcgtacacc ggcgtcagcc
       61 gcgccgacac gatcaacgtc cccaccacgc tcatccccac aaacgccacg atcttccacc
      121 gctgccgctt caatatccat aggtagtgag acaggggaag agccggcttc tctgggtcgg
      181 gctcaatcag gggcggcggc gcccagacgg agacagggta ttgttcggcg actgcgggca
      241 agttccgttc tgtgatttgt gcctgcatgg agtcctcctc gttcgtacta gcgccgccaa
      301 ataaggattc cggaggcggt ggcggttccg aatccggcga tcttctcgag tgtgtgcacg
      361 gcaacccgcc ggccgctgtt gtccgggatg tagagaatgt cgttggcgtc cagaggaatg
      421 tcgggcgcct tacgttccat gatctgtttc agttcgatcg caatctcgtg cttggacccg
      481 gtagccgctt cgcggcgata gatatatgca cgcttggcag cgaacgggac gagaccttcg
      541 gccaaggcca gcatttgcag cacggttgtc tccgattcgc cctgtacggg atacacgccg
      601 ggtttcttga cgctgcccac gacaaagatc ttccccacct ccggcacacg aatctcctcg
      661 cccccggcga gcttcaggtt cagttccgga tcggccgcgt cgatcaggcc tctcaccgga
      721 atccgctgta ccagcgtcgc cggcttgccg tcctcccccg gttgcgtccg gctcaccaga
      781 atctccggcc ccgcgtccac gctcaacccc ccggcccgcg tcagcgcgtc gagtaaagtc
      841 atcgggcccg ccgcctggaa cgtgaccgga tttcgcaccg ctccggcgac gctgattggc
      901 cggctgtggt actcggcaat cgtcaccgtc accaccggct ccacaagcaa ctgttccgcg
      961 tggagtgcct cggccaccgt cgcctcgagc tccgccggca acagtccgtc ggcgcgcact
     1021 cgccgcttga gcatgggcag gcggatgaag ccgtcggcgc caacgcgcac ggtgcgcgtc
     1081 agttccggcg cgtcgtacac cgagatggcg atcagatcat tcggcccgat cttctgagcc
     1141 ggaaggtttg ccgctccaag ctcggatgtg gaacggacct gcgccgaaag ggcgccgcac
     1201 agtagcagaa acaggattgg gagcatcgtt gacacctcat ttggttggag acaccggatg
     1261 accgctccga acggagcgga accgctcagc ggatctgttt caggacacgg ggccggctgg
     1321 atcttacgat tccgcgccgt cgttcaagag gcggatcagg gaggtcacgc gtgattccag
     1381 ttcagccacc ttgtgttcca ggttggacag ggggctttgc gccggaccgt gggacgcctc
     1441 ggtcgcgcca gccgagcgca acacggcggt gcgtgcaaaa tccgaaatgc tacgagcgcc
     1501 cgtgaatgcg tagacagcct ttaccgtctc gtactcctgc tggctgagac gaaagttgac
     1561 cagacgattt ctgggcctga gaacggacat ggaaaatggg gcgaatatga gaagcggata
     1621 cggctagtga gctagcgccg gccggcccga cggccgcagc acatcgcggt cgatctcgac
     1681 ggccacggag cgctgcagca gatggacgct caccaccacc cggcagacgt ccttcaattg
     1741 gacgaggatt ccttcaagac ctgacagcgg accggcttcg atgcacactg tctggccgac
     1801 tttgagaaac ggccacgggc ccgacggaag atcggacgcc acaatggacc ggactgcatt
     1861 cagctcctct tcggcaacag gcgcgggtgt ccggccgaag cccacgacag agcgtacgct
     1921 gggcgtcgcc agcacccggg cccgatcgcc ggattcaaag cgggagaaga cataccccgg
     1981 aaagagcggc acgtcgagtt ccttgacacg gtccgaccac cgccggcgcg cccgataaag
     2041 aggcaagaag aactcgaggc ctttggattc gagcgcctgg gcggccgctc tttcgtgctg
     2101 gggctttacc gtcaacgcaa accagtcggc actgtggtac gaatttggga gaggattgcc
     2161 aaaaagttcc ataatcgagc agctaccgcg gcacgccaca gttcttgtcc ggcggcgcac
     2221 cgaattccta cggctcaagc ccccggccca ggccgattca tcggctgatg gtttctttct
     2281 ccttctgcgt caggtagact gccgccccgg cgccggctcc gccgacaatc acgccggcga
     2341 tgacggcctt cttcgcgccg gacatcccgc ccgacggcgc ggacaccgga ggagtgggct
     2401 gcgtgaccgg cggggcaggt gttttcgcga cctgtggagc ggccttgggc ggtgcggtct
     2461 tgggccgagc cgcctcgggc ggctcggccg gtttcgcggc cggaggtacg gcggctgaag
     2521 gcggcggcgc agggcagccg cggcgcacgc gtctcatcct ggtcacctgt acgacctcca
     2581 tcgcgccggc caccggctgt tctttgggca actcgaaggc caccacttcc acgacgttgc
     2641 cgacctcccg atccagcctg tcgccgcgaa tctcctcgag gactccggca atcaggtccc
     2701 ttagcacaaa acggccgtcc cgccgctcca cgcagccggt catctcgaac ggtaattgcg
     2761 caccggctgg agcctgtggc tggaactcga gggccatccc cggcgccatc accgccacca
     2821 ccgatccgcc ggtgttcatc actcgcagac cgccgcgtaa agccgcgatc tggacactcc
     2881 gctccccgga ataggcgatt cggcctgccg aagcgccgcc ggtcggaacg atcctcaatc
     2941 caagcgcctc gaccgcatag ccccggttgt tgtcccactc gccgtgacct tgctcgagca
     3001 ccagcctgcc gccgaaaacc ttcgccctgg aaccggagtc caagagtaca cgaacgcctc
     3061 cctgaatccg cagttcggaa ggatctgtaa acgtctccag ggtgttgccg tcgaaaatgg
     3121 tagcgttgcc ggagacggta tgattgtcga gccgaaattc gccctttgtg atggccactc
     3181 cgatcgaagc cggcgcctcg gccccaatcc ccaccggata cagaaagaga gcgagtaagc
     3241 acgcagccgt gagacgatgc acaacacgac ctccgtaggt tgtactctgc gtgcgggttt
     3301 taggcatcat tccacgcctc tcactctctt gtcaagaacc cggccagacg ggctcgctgt
     3361 ctgtcggaac gaatgcggat agagctcacg ctccgtgacc cccattcggg aacctgttcc
     3421 agaggtcccg cagcgtagcc cttgggctgg tgcctcttcg cgggacaaat cattaaagag
     3481 cgccgcttta cagtgagttt acggaagcaa tcaacgctct caccccattc atcggacggc
     3541 aagccgtaga acttcagacc gtcctcgggc tggtacggct atctgtttga cccaccaaga
     3601 gatctcgcac cgaattctct gcggaacatt cctccccctg agacgtatat gaaagtgcgg
     3661 ggaaaaacag cagcccgcaa tcctagaatc acggggactt cataaatggc aggcaacggg
     3721 agcaacggaa acggaaagaa caggcgccgc cgcaggcggt tgatatggct tagcgtcctc
     3781 gcgatgctgc ttgtcggcgc tttcttcggc gtgcgggcag cgctgcgccc gaacaatcac
     3841 atcgacccgt cgaaactggc ggaagtgacg cgcggcgata tcgcacgctc cgtagtggct
     3901 acgggaaaaa tcgagccacg cgccaaagtt gaagtcaagt ccaaagccag cgggatcgtg
     3961 cagcgtctgc tggtggacta cggcgactgg gtgaagcagg gccaggtcct tgtggaattg
     4021 gacaaggaag agcttcaagc ccgtgtccgg gaggcgcgcg ccacgctgct ggcggcccaa
     4081 gccgctgaag aatcgtccaa ggcgacctat gagcggaaca aggtagaggc cgaaggcccc
     4141 gacatcccct tcctcaagtc aggaatggac cgcgcccaga agcttcacac acagggattg
     4201 atcgcgaggt ccctcctgga agacgcagag aaggcctacc agacggcgct gaacaagcag
     4261 atggctgctt tgcgaaacct gtccgtcgcc cgcgcggaga tcgcccgctc tcaggcccag
     4321 gtagcgcagg cgagggctgc cctcgaacgg gcggacgaag accttcgtaa cgccaccgtc
     4381 gtgagtccga tgaacggtct cgtactctcg cgcaatgttg agacaggcga tgcggtaagc
     4441 tcgattctgg ttctcggctc tcaggcgacg ctaattatga cgctgggcga tgtcagtgac
     4501 gtatacgtcc tcggcaaggt ggatcaggcc gacatcggca aggtctacat gggacaacgg
     4561 gcccgcatcg tggtggagtc cttcaaagac aaaaagtttg aaggacaagt tacgaagatc
     4621 gcgccgctcg gcgtcgagaa ggagaacgtc accacctttg aggtcaaggt ctcgattcgc
     4681 aatcccagcg gtgagctcaa ggcgaacatg agcgccaatg ccgagatcat cctcgaggaa
     4741 aagcagaacg ttctgctgat cccggagagc tccatcgtct acgaccggaa ccggaatacc
     4801 tccatcgagg tgccggattc aaccatcgaa gccggacgcc gaaaagtgcc cgtgaagctt
     4861 ggcatctcca acggcgttaa aaccgagctg atttccggcc tgaagcccgg cgagaaggtc
     4921 gtgcttcagt aggccgcccc tgggatcctc acacaagccg ttccaggaga gagatcaggc
     4981 actcctggtc aaagtccgag gtgcgatgag tgcggggatc gaacaggagc caggtctgcg
     5041 agtctttctc gaggcagtaa aacagcaggt gctcggggat caccgcgcgc tcgatggatt
     5101 cctggtttag acagcgcacc tccccttgga ggacgtacgg cacgcggtac tgttccgttg
     5161 agatcggggt gtcggtggcg cggaactcga tctggatcag ccgcccggcg atgttcacct
     5221 gaaacaggtt ccccccggca tcgcggtaag tgtcggccgt gaattcagcg ggtgcgagat
     5281 cgaccagagg cttccggagg agctggttca ctgcgccaac gaatgcggcg catgtcgcgt
     5341 gcagttcacg ggctccctgg gaccgcaatg cggcgacctt ccgctcttcg cgaattcgct
     5401 cttcgtcctt ccgaacgagc gtctcgatgt tgttggccag cctcttcagc cggtcgtccg
     5461 caccggtgct gcgcctgaac ttcatcaccc gagatacaat cctcccttga ggacgaaccc
     5521 aagtgagaat accatactcc gcgctgatcg cctcctgcat ctgcgccgcc gggctttgcg
     5581 cgcaggaccg cccgggcctg ttcttccgcg aggactggaa ggagactcca gcggaagtcc
     5641 ctgtaggcca ggctcacgtt gttaatccgg acctcgtgtt gggcctttac ggccccggcc
     5701 ggacggtaat caagaagagc caccacgaca ctccggccga cgatccgtat tacgtctggt
     5761 ccggcctgtg cccgctaaac tgggcggtaa cactcaagca caaagacgca ttcgccgacc
     5821 tttccggcca ggcgaagctc cgctggcggt cgaagcaggc cggcttccgg cagcttcgga
     5881 tcatcctgaa gctcgcgggc ggcgcgtggc ttgtcagcga cgcctatgac ggtccgtccc
     5941 aggactggcg cgtgcgtgag ttcaacatcc aggacattcg ttggcgcaag ctggacatcg
     6001 aaaaggtggt cgaaggcgcg tgggtggaga ggcccgacct gagcagagtg gacgaaatcg
     6061 gcttcaccga tttgatggtg ggcggtgaga gcatcgcctg ttcccggctc gactggatcg
     6121 aggtcgacgc gaagccggtg aagcgcaggt agtctggcgt acagcccaag cgaagtatct
     6181 atttttctcc gggcctctcc ggccacagtg cgttccatcc aggctcgggt accggcttca
     6241 cctttaggac ttcacaggcc ctgaggtgcc gctcccagag catcgcccgc cggagacgtg
     6301 cctgccgggc cacgcgctcg gacgccgccc tctcaacggc cggcgggccg cccgcgagcg
     6361 gcatcaactc cgcgatcaag ccacccagtc tctcgcgctc ggcaagcagc cgcgcaggat
     6421 ccatcgtgta cttcgagaat cccgacacga tctcggcgtc caccttcaat agcgcctcgg
     6481 ctttcccgat aagagcggtg tgggctgcga ccgtccctcg cgaacgaagc gcctccaccg
     6541 cgtcgcgcag caccatgtgg ctctcccagt cttcgatgcc atcccgaatt gcctcgaagc
     6601 gcagcgagga gatcggcccc gggtagaaca gcattccgtc gccgtttgtc ggccagccgt
     6661 ggctccaccc gtcggccttc caaggaacgt tcgggtaacg cttcgacgga tctttggcgt
     6721 agttccgttc ccagatgttg taacagtaat actcgaagcc ggtgacgccg tacttccacg
     6781 tcgtccagaa caggacccgc gacgcgatca gcggattctc cacctgcacg ttcggatacg
     6841 gataggcagg cccggccgcc gtgtaccagt agaactcctc gcctctcttc tgcgactccg
     6901 cgatcagatt cacctggtct tcctggaggc tgtacacttt gctccggccg ctcacctgcg
     6961 ggtcgtagat atcgacgaac ccgaccagtt cttcgcgagg aaagctcccg ccgatcatca
     7021 cctgcactcc cggcagatgc gcgtggacga ggtcggcgcg cctgcgcatc tcgttccact
     7081 ggtccgtctc atcgccgatg tagaccagcg tgtagtcgag cgcccctagc ctcttcaccg
     7141 tctcgtagtt cttcttgagc tgtgcgagtt ccgtgtccgt accggcgaag tttccgctca
     7201 gatacaccgt gttcatgccc tgccgcagca actcctcgag atctttccgg ggcgagagga
     7261 tctgggcgta ctgctcagtc gggctgaatc gatgctccat gaggaagtcg tagtacttcc
     7321 gatacagacc cgcgtccgga tcttctccgt ggaaacgcgc tgcgttgccc caaatgaact
     7381 gtcccatcga gtgaaaattc cacttctccg ggatggcgta cggccatacg ttcacctcaa
     7441 gctccaggtc cttgagcata cgcgccgagt ccgcaatcgc gattttgccg cggtacaccc
     7501 ccggagacgt acccggacgc gtgcgcacaa ccacgtagaa cggcgtatga acgtgccgcc
     7561 gcagcctcac cggctttgtc ggcaacaaag ggtccgtcgt ctcatacacc ttgccgccga
     7621 acatcagttc ccgggtcatc cagttgcgcc gcatcggata gacgtcctcg atgaaatagc
     7681 tcacgtcgga tgccggtatc gtcttgcctt cggcgcttac gaggtccgac acgttcagcc
     7741 gcactccctc gatgtcccgc agcatcggtt gaaccacgac ctggagaccc tcgaactcgt
     7801 tcgccgccat cgacatccgg tatgacgtcc cgtacgatcc tcggtacccg aacctcgtcg
     7861 agaggtcgca gatgcgtgtc atgccgtccg tcattccggc gaccacctca tcgccgtcca
     7921 gttcgagcac cagcttcgga tgcagcttgt cgccgctgct gtacgtcgcg gtgggcacgt
     7981 cgatctccca agtgctgggt gtcctccggt ccggcgggtc ggccggaatg agcagcaccc
     8041 cgtggttggc cgcttccccg gcgatccatt tctctaccag cggagtgatg ttgaactcga
     8101 tccaccggcc gccttccgga acgcccagcg ggaggaacgg gaaatctggc cagccctcgt
     8161 gcggcggagc gaacagctcg accgaatcaa ccgccccacg cctccgcccg ttgccgtaat
     8221 cggtgagttc gtcatacgaa atctggttgc cgccgctgat cgcctcatgg cagaagatct
     8281 cggtgaactt gttcggcttg cgcgccgagg gcagaaaaat catcagccgg gcgcgcgcga
     8341 gtgtcccgct cagcttcggc agttcgaacc tcgcgtgcag gctgttctgg gatccgcgca
     8401 gatgaaagta cccgtcgtcc gcctccgccg caggtcgcgt gtcgacgatc gtccgtgtcg
     8461 tgctcccctc gtaaccgtcg accccctgtt ggagggtgac catcgaaccc tcgctgagcg
     8521 tggctgccag gaataatggc gcaatacacc gaatcattct gcgttctcct tatgaatccg
     8581 aagtgcagcc cgcacagagc gcacggaatc ggcttcactc cgccaatagt agaccaaaag
     8641 gggaggaagt ccgctattcg tagcgcagcg cttcgattgg atccaggcgc gcagccttga
     8701 tggcgggtat catccctgcg accaagccga caaaaagcag cacgccggtc gaaaccagga
     8761 tggagcctag cgacaggccg agttcaacgt tgcctttatc cggagcgtcg tccatgatcg
     8821 ccccgaacaa aggcatcgat cccagggcga aagtggccac ggcgcccacc gcaacgccaa
     8881 gaaggcctcc gatgccgacg atgatcagcg cctcgaaaag gaactgctta aggatcatgc
     8941 ccttggggcc gccgagagcc tttaacacgc cgatctcgcg cgtccggtcg agcaccgttg
     9001 ccagcatgat gttggcgagg ccgacgcctc cgatgcccag tgtcagcgcg ccgacgaaac
     9061 ccagaagcat tctcgccgcc atcgacatcg tttcgatgat gtacaggaac tggttgaaag
     9121 ccagaatgaa gatcgccttc tcgtcgttcg gcgagaagcg gtggatcatg cccagggtcg
     9181 cacgcacatc gcgaatcgcg cggtctcgca cctgcggcga gatcggagtc cacacgatga
     9241 agtcggggta gcggaggtcc ttgtagatgg acattgtgtc gtaggggatg aagatgcagt
     9301 tgttgtcagg cgtattgtag ttggcaagtt gtcccttcgc gtccagcacg ccaatcaccg
     9361 tgaaacgctg accttgcagt gcaatctctt ccccaaccgg aggaatgccg ctgaacatct
     9421 ccttcgccac cttcgcgccg agcaccgcca ctcgattccg ttgcagggtg tcttcccggc
     9481 cgatccagcg ccccgacacc atctgcatgt tccggatgta ttgatattcg tgccagaccg
     9541 ctcgcagcgt gtactgcttc tcgcgggtgc cccgaacgac ctttgttccg tacttgagca
     9601 gctccgggga catcgcgccg accgtgggta ccgcctcgcg gagcgtctcg acgtcgctca
     9661 gttcgagccg cacctcccgg ccggagcgca tgccacccgc ttgcaggctc gtctggcccg
     9721 ccgccatgat aatcaggtcc tgcccgacgg cctgaaagcc ttgcagcaat gcctggtcaa
     9781 atccgtgacc gtacgacatc agcagaacga acgaagccac tccccacgcc aggctgagaa
     9841 tggtcaccag cgtccggcgc cggtaaaacc ggagagaact caccgcttcc gagatctgcc
     9901 accgccaggt catggcatca cctctcttcc cggagcgctt caaccggcgt gagctccgac
     9961 gccctccacg ccggcggcag agccgacgcg agcgccaccg tgcccaacgc agccaccgcc
    10021 agcagtgtgt tgcgatagct tatgggcagc cccgcgaaca tcgccggcat cggcagcgag
    10081 ttaaccgccg tggccagcgc gcccacgacg gccagtccga gcgcgccgct tagcaccgcc
    10141 agcagcaggc cctcgaggaa gaagtccagt aggatgcgcc ttttcgtcgc tccgagcgct
    10201 tttttcaagc cgatctcgtg ggtccgctcc gacaccgccg tcatcatcgt gttcatgacg
    10261 ccgactccgc caaggctgag cgtgatgatg gcgatcaccg caagaaagat ctcggtggcg
    10321 ttgaaaaccg cgccgaacag ttccgcgctc tttaccgtgt cccacatcgg cagggcgctt
    10381 tcgtcaagcg ggtcgaaatc gtgcaggcgt ccgagcgccg tccgcacctg acgttgtgcc
    10441 ccttcccact cggtgatcga cagcggcctg tagacgaggg tctcgattct gcctttctga
    10501 tagtcggacc acttgggagg gcaatcgcgc tccaggcttg tcttcggaat caggattttg
    10561 tcgttgtccc agccgtcgta gctgctgttc tgattcttct tactcatcag cccgacgacg
    10621 tggtacggat tgccgtagat ggacacctgc cggcccaaca catcgccgcg gtaaccgaac
    10681 aactgctcct tgacggaggc gccgagaacg cacacgcggc gggcgttctg gttgtcctcc
    10741 cggttcaatg tgcggcccgt ttcaacggga aggtttcgca gttgcaggta ttccggcgaa
    10801 gcgcccagcg ccagaaacag gccggagttg aattcgctag tggccgcgac ctcgcgcttg
    10861 acctcgccgg ccaccaccga gaccagcggg cactgttcgc ggactacctg tacgtccttg
    10921 tcccacagcc ggatgtggcg cccagctcgc tggccgcccg cctgccgctc ggtgcggcct
    10981 ccccacacca gcacgatact ctctcctaac tgcttcatat tgttgcgctg gccctggcgg
    11041 aagccatcgc agagggcgct catcagcacc agggacgcga tgccccaggc aatcccgaac
    11101 atcgtcagga agctgcgcag cttgtggcgc cagacattcg caaccgtttg ctcgatgaag
    11161 ccgtagatta cgttgagaaa ccgcatcgaa attcgggaca tgctcggtcc caattccttt
    11221 gtacgcttcg gaggagagaa tagttcccgg aagtcggatc cgggccagtc tgctgtcggt
    11281 gataggctac tgcacttttg tcgccgaaaa ggtcccggcg taaccggcgt actcccaatt
    11341 gccggtgacc tttccgccgt cgaccttccc ggtcagcttc agttcgcctt caaggccgcc
    11401 ttcttcatag acaaggtgga acttcagttc gaacttgcgc tcgctgtacg tccctttgac
    11461 atcggccctg tcggcaaacc tgcccgtcac ctgcccgcca tcgaccttaa aggtcgcggg
    11521 cgcctggcgg tcgccgtcgt cgctgttgaa tgtaaaattc cagttgccgt cgagatcggt
    11581 cgcgcaaaat gccggcacgg cggccagcag cgtacagatc atcagtgcga agacaccagt
    11641 cagtcggaac acacatgcct cctttcgggg caaacacaga gtctatcaga ggacggcacc
    11701 ggcttcagcc cgtgtttctg ctagatcgtc attgccagga aaccaccgtc cacccgaaca
    11761 atggaaccag tcacgaacga ggacgcctta tccgacgcca ggtaaatcgt cgctcctaca
    11821 agttcttcgg attcgccgaa gcggttcatc ggcgtgtggc tcatgatctg cgccgtccgc
    11881 tctggagtca ggatgcgacg gttttgctcg gctggaaaga accccggcag gacagcattc
    11941 acccgcactc gatacggcgc ccactcgcgc gccaggaatt gggtgatctg gttcacccct
    12001 cccttggtta cgctgtacgt gaataccttc gacagcggag gacccgacga tgcggatgag
    12061 atgttgatga tcgatccgcc ccggcccgac tccaccatca ctttcccgaa cacctggcag
    12121 gcgaagaaga cactcttcag attcacgtcc agaatccttt gccactccga ttcctggatc
    12181 tcgaaaaacg gcgtcgccga gttgatcccc gcggcattga tcagaatgtc gacgctgccg
    12241 agttcgtcca gaacctgccg gcatgccgct tcgagcgccg gtttctgcgt cgcgtccgcc
    12301 tggattccaa gcgccttccg gccaagccgg cgaatcgatg tcgcgcgatc ctcggcgccg
    12361 tctttgttgt aatcgacaat ggcgacgtcg gcgcccgccc cggccaggcc ctcggccatc
    12421 gccccggcaa gcacgccgcc accgcccacc actacggcaa ccttgtcagt gagagagaaa
    12481 agttcagtat gtgtcatcga ttaagttttt ctatcttctc tactctatat cggggatgtc
    12541 cgccgggcca ccaccaaggt gacgtcatcg gccggggccg ccccggccgt ccactctatc
    12601 agcgcgttcc gcaggtcatc cacgattgcc gcagcgggcc tgccgcgccg ttcgaccatg
    12661 agtgcggcca ggcggtcctc gccgtactcc tcgccggacg ggctggcgat ctcggtgact
    12721 ccgtcgctgt aaagcaccag tgtgtcgccg ggcgcaatca gccgcgtgtg ctgctcatac
    12781 acagtgtccg gaaatacccc gaggatcggt ccgcctccgt ccagccactc ggtattgccg
    12841 caagcgcgaa ccagcagcgg tggattgtgg ccggcgttgc agtacgccag ttctccgctt
    12901 gccgggtcga cgattccgag aaacaggctc acgaaccggt tgctcagcga ccgggcagtg
    12961 agcaggcggt tcagccggtt cgtcaagcgg gagagatcgc tcaggtcctc cgccaggact
    13021 tgcatgaaag cgtgcaggct cgacatcagt agcgccgccg gcatgccctt gccggagacg
    13081 tccgcgaccg ccaggccgac tcggccgtcc ggctgcggga agaaatcgta gtagtccccg
    13141 cccaccgtgc ggcacggcgc gttgtgaccg gccaactcga gtccggaaac cggaggcggg
    13201 ttctcaggca gcaggcgccg ctggatctcg gccgcctgct ccagttcgcg cgccaggatg
    13261 cgctccacct gctccacgtc cgcgagtcgc gcatgctcaa tccggatcgc cgccacattc
    13321 gccatcacgg tcagcaggtt gaggtcctcc cggctgaatt ggcgcactac gttcggcgaa
    13381 tcgacgtaaa tcaggccgat cactctttcg ctcgtctgga gcggcaccgc cagcatgctc
    13441 cggacgttct gcgcctgaat gctcttccgg gaacgaaacg cgtcttcgcg ctgtacgtct
    13501 tccaccagca gcgactcgcg ctgctggacg acgcggtccc gcaccgcgct gctgatccgg
    13561 aagctgtcgc cgcgcgccgc ccgcaccagc aatcgctcgc cttcgagcgt cagcaggacg
    13621 ccacggtctg cgtccaccgc gtccaaagca agatcgagga tcagcggaaa cagttccccg
    13681 agcggccggt gccccgccag ttccctaccc gcatgaacca gtgcgcgcac ccggatgtcg
    13741 agatcgaacg gtgatgggct cgcggtctcc acgaacacgg gctcgctccc gagcacgccg
    13801 tccaggttgg cgatcatcgc cgtcgaagcc ggaacgtccg ccacggtcgg gtccacgaac
    13861 acgacgctct ccgccatctc ccccagtgga tcgtcgaaaa ccatgaccag gtgtcccgcc
    13921 gtgatgcgat ccccgggctg gagccggtgc ggaccgggga tccgctcgcc attcacttcg
    13981 gtcccgttct tgctgccgag atcggcgact acccactcgt ccccatcccg ctcgaacaca
    14041 aggtgctgcc gtgacagacc ggcgtcgtcg gcaaagcaaa gctcgttgga ggtggcccgc
    14101 ccgagggaca cacggctccc ttccagacgg accgtctggg atctgccttc cggggcgc
//
LOCUS       DATIFQ010000358         7712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2416750, whole genome shotgun sequence.
ACCESSION   DATIFQ010000358 DATIFQ010000000
VERSION     DATIFQ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416750"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VLE22_17040"
     CDS             complement(<1..847)
                     /locus_tag="VLE22_17040"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HSB16163.1"
                     /translation="MMFGRRLRLGAVVVCVCGISVAAGAAQQPPAQQPPTAPPKKQSP
                     FEAVQGAPDEQAKPQQPPPAKPPSPFEAPAPAAEPAPAAKVEDVIEAIEFRGARRVPQ
                     DTLRAMIFSKRGDRIEEDALRRDFMALWNTGRFDDIRLEIEPGKAGQIVRYVLVERQI
                     VRSIKYDGMKSVTVSEVLDRFKERRVGLTVESQYDPNKVQRARVVLQEYLAERGRQFA
                     EVNPEITPFPPSSLSVTFKVKEGPKVKVGKIDIENNKVFSDRAVIRAMKNLRPIGIPK
                     SILFES"
     gene            complement(1001..1483)
                     /locus_tag="VLE22_17045"
     CDS             complement(1001..1483)
                     /locus_tag="VLE22_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16164.1"
                     /translation="MEPVKCNPWKNLAIEAAVGVETGVLAGLASLSWLALHCAVKGRP
                     AWVAPAEIAALVRNDLANRPFFGLAMHIVASGFLGALLCVPAGRWLRSGRTLLPGLMT
                     GVSIYVAGNLPLAGGRRLLVPMPLPDWSILVACLAFGVVLGAYPALLHEAQGKAEPPD
                     "
     gene            complement(1513..1791)
                     /locus_tag="VLE22_17050"
     CDS             complement(1513..1791)
                     /locus_tag="VLE22_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16165.1"
                     /translation="MFANLLSLTLREPGGERPVPRGWLEQFFMRDFTGHSAFDQTLAA
                     GDGRLEVGFAVSSEAVREEFEKWLRGRNMITQGTALEVRETGSERNLK"
     gene            complement(1799..2662)
                     /locus_tag="VLE22_17055"
     CDS             complement(1799..2662)
                     /locus_tag="VLE22_17055"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HSB16166.1"
                     /translation="MSRVHDALRRAEQAGDVRPRAKRPSSGRMPGRDFLELLEQVQEV
                     PYNPSPDSYLVDPSRPHDAHSEEFRTLRTRLNHLQKMQPIHTVVITSATPAEGKSFTV
                     VNLALSEAQLTGNLTLLADFDFRRPVIHNLFQIDRTPGITDYLRGDVPLSAAIRRLAG
                     TNLYVAPAGESVTNPLELLNLPSAKDLLEQLPNVFNWVLLDSPPLLFAADANLLATMC
                     DGTIMVVRVGTTTIDSVTRAMQTLCENNVLGVVANGARKGELYSKYTYYYSSYYYKGS
                     EKEKEDPEPQE"
     gene            complement(2684..4345)
                     /locus_tag="VLE22_17060"
     CDS             complement(2684..4345)
                     /locus_tag="VLE22_17060"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HSB16167.1"
                     /translation="MQPTPESYNVQRRSMDVEDYVDVVRRHRGWIAGPAFAGLVIAVV
                     VAFLWPDTYVSMAVIRVVPPQVPEKYVASNVNIEMTQRFSAMAQSITSRARLTDMVNA
                     HGLYPGLRSRVPMEDVLERMSKDIKINPVFSLRDEGMQGRPASSAYRIEFAYENRYLA
                     QKVVQELVSRFIDESITTRASQSVMTTDFLNDKLAEAKRQLDEIENRLTEFKMRNMGR
                     LPEQRDSNLQQLRTLETQLSAMSSSINRATQEKLLLESQLRTLRDQQQRVPTTPGNTA
                     ETAAKSERLMQTEKQILDLETMLSGLRQQYRDTHPDVRRVQAQLAVLKKNRDALLDEE
                     VKREAAVPKTKETAPIRTREGAELEAAIQRQQSFIRAKDMEIEQYVKDQGRLDKAIRS
                     FQDRVEAIPMGEREYAELTRDYALARNRYEELSQKRANSEIATDLESRKQGETLELLD
                     PATLPQTPTEPKRWLIIAIGTWLGLVAGLFLAGMREIRDTSLKNLKDVRAYTGLTVLG
                     SVPLLENDLVVRRKRRLTWLAWSSACIVGFLVMITSVYYYYTKQA"
     gene            complement(4381..4983)
                     /locus_tag="VLE22_17065"
     CDS             complement(4381..4983)
                     /locus_tag="VLE22_17065"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HSB16168.1"
                     /translation="MSSFVPPTPAQEKQQSSQQQQSPGAPVTPEEEALRKAVGAPIDP
                     KNYVIGAEDILMIRVWREPELSGPVGVRPDGKISLPLLGDLQAGGLTPEKLAQSITQG
                     FSKYVTNPEVMVQVASVNSKKYFISGEVMRPGSYPLVVPTTILEALAVAGGFREYADR
                     KKILILRGPKRYTFNYKEVVRGRNMQQNMLLESGDHIIVP"
     gene            complement(5130..5699)
                     /locus_tag="VLE22_17070"
     CDS             complement(5130..5699)
                     /locus_tag="VLE22_17070"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HSB16169.1"
                     /translation="MLVLASQSPRRQEVLTRAGIPFLVRPAEVDESRLEDESPEEHVR
                     RLAREKVEATAARPGDVVLGADTVVVVDGEVLGKPEDTSDAERMLRKLSGREHVVMTG
                     VCVRATGGTEVDVESTVVRFVALSEEEILDYAASGESMDKAGAYAIQGLASKFIDRIE
                     GCYFNVVGLPVAMVYRKLKQLGVPPEEEF"
     gene            5818..6162
                     /locus_tag="VLE22_17075"
     CDS             5818..6162
                     /locus_tag="VLE22_17075"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HSB16170.1"
                     /translation="MTPEDLARAAWPLAVGKTVAAHTRVFSMVRNSLIVEVEDHVWRR
                     QLFVMSGQILRKIEEVLGSPLVGALEFRVGLSKRPPQRAEQPRPSADEADGIEDPVLR
                     RIYIAKRKRETA"
     gene            6159..6458
                     /gene="gatC"
                     /locus_tag="VLE22_17080"
     CDS             6159..6458
                     /gene="gatC"
                     /locus_tag="VLE22_17080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HSB16171.1"
                     /translation="MKITEEQVRYVADLANLRLTADEIRRLRADLDEILAHMDKLNEL
                     DTAGVEPMAQVLYEAEETATLRDDIERPVLGTDVALANAPQSGAGYFKVPKVIER"
     gene            6462..>7712
                     /gene="gatA"
                     /locus_tag="VLE22_17085"
     CDS             6462..>7712
                     /gene="gatA"
                     /locus_tag="VLE22_17085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HSB16172.1"
                     /translation="MNIASLTIGNVREGLLSRQFSAEELAAAALRHAETENPATNAYL
                     HFCPERALASARRVDARLARGEDPGPLAGVPIAVKDVILTKGVRSTAGSRLLEHYIPP
                     YDATAVVRLEEAGGVILGKTNCDEFAMGSSNENSAFGPVRNPVDPERVPGGSSGGSGA
                     AVAQGTAVVSLGSDTGGSIRQPASFCGVVGITPTYGRVSRYGLIAFASSLDHIGPFAR
                     NTRDAARLLQVIAGRDQMDSTSAFAPVPDYTALLDGNVKGLKIGLPREYFQGLASETG
                     DLIARGVDVLKRLGCQVREISLPATDYAVACYYIICTAEASSNLARYDGVRYTTRSKD
                     AGTLIEMYRATRGEGFGAECKRRIMLGTYVLSAGYYDAYYLKAQKVRALIARDFAAAF
                     RDVDAIVAPVSPFPAFKLGEKIDDP"
BASE COUNT         1332 a         2703 c         2161 g         1516 t
ORIGIN      
        1 gactctcaaa caggatcgat ttcgggatgc cgatgggccg caggtttttc atcgcgcgga
       61 tcaccgcgcg gtcgctgaag actttgttgt tctcgatgtc gatcttgcca acctttacct
      121 tcggcccttc cttcaccttg aacgtcaccg acagcgaaga cggcgggaac ggcgtgatct
      181 ccggatttac ttcggcgaac tggcgtccgc gctcggccag gtactcctgc agcaccacgc
      241 gggcgcgttg caccttgttc ggatcgtatt gcgattccac cgtcaggccg acgcgccgtt
      301 ccttgaaacg gtccaaaacc tccgagaccg tcaccgactt cattccgtcg tacttgatcg
      361 agcggacaat ctgacgttcc accagaacgt accgcacgat ctggcccgcc ttgccgggct
      421 ctatttccag acgaatgtcg tcgaagcgcc cggtgttcca cagcgccatg aaatcgcggc
      481 ggagcgcgtc ttcctcgatc cggtcgcccc gcttggagaa aatcatcgcg cggagggtgt
      541 cctgcgggac ccgtctcgct ccgcggaact cgattgcttc gatgacgtcc tcgactttcg
      601 cggccggggc cggttcggcg gcgggagccg gagcctcgaa cggcgacgga ggcttcgccg
      661 gtggcggttg ctggggcttt gcctgctcgt cgggagcccc ttggaccgct tcgaagggac
      721 tctgtttctt tggaggggcc gtcggaggct gctgtgcagg tggctgttgc gcggcgccgg
      781 ccgcgacact gatcccacac acgcatacca cgacggcgcc caggcgcaga cgcctgccaa
      841 acatcatcta gacgcggttc ctttccactc tgcgcctcaa tggagacgca caagactact
      901 ctctggtttc acgcagttct gaaacgatct actggcgcag gtcgtgaatc cttattctaa
      961 cccgaacgcc gggccggttg gcaggcccgc atgcgcgaaa tcagtccggt ggttccgctt
     1021 tcccctgtgc ctcgtgcagc aaagcgggat aggcgccgag gacgacgccg aaagccaggc
     1081 acgccaccag aatcgaccag tctggaagcg gcatcggaac cagcagccgc cggcccccgg
     1141 caagcggaag atttccggcc acatatatac tcaccccagt cattagcccc ggcagcagcg
     1201 ttcggccgga ccgcaaccac cgccctgccg gaacgcaaag cagcgccccc aaaaacccgc
     1261 tggcgacgat atgcatcgcc agcccgaaga acggccggtt ggccagatcg tttcggacaa
     1321 gcgccgcgat ctccgccggc gcgacccatg ccgggcgtcc cttcacggcg cagtgcagcg
     1381 ccagccacga taaggaagcc agtcccgcga gtactccggt ttccacgccc acggctgcct
     1441 cgatcgccaa gttcttccac ggattgcact ttactggttc cacggattgg cgctacctcg
     1501 ccccggctac gcttacttca gattcctttc actcccggtc tccctcactt ctagcgcagt
     1561 tccttgagtt atcatgttcc tgcctcgtag ccatttctca aactcttccc gcacggcttc
     1621 cgaggatacc gcaaatccaa cttcgagacg cccgtcccct gccgccagcg tctgatcgaa
     1681 tgcgctgtgc ccggtgaaat cccgcatgaa gaactgttcg agccagcccc gcggcacagg
     1741 cctttcccca ccgggctcgc ggagcgtcag cgacagcagg ttggcgaaca taactcgcct
     1801 attcctgggg ttccggatct tccttctctt tttcgctgcc cttatagtaa tagctcgaat
     1861 agtagtaggt gtacttgctg tagagctcgc ccttccgggc gccgttggcg accacgccca
     1921 ggacgttgtt ctcgcacaac gtctgcattg cgcgcgtcac ggaatcgatc gtcgtcgtgc
     1981 ccacccgtac caccatgatc gtcccgtcgc acatcgtcgc cagtaggttc gcgtccgcgg
     2041 cgaacagcag cggcgggctg tcgagcagca cccagttgaa cacgttcgga agctgctcca
     2101 gcaaatcctt cgcgctcgga agatttaaca actcgagcgg attcgtcacc gactcgcccg
     2161 ccggagccac gtacaggttc gtgcccgcca gcctgcggat cgcggccgag agcggcacgt
     2221 ccccgcggag gtagtcggtg atgcccggtg tccggtcgat ctggaagagg ttgtgtatca
     2281 ccggccggcg gaaatcgaaa tccgctagta gcgtcaggtt gcccgtgagc tgcgcttccg
     2341 aaagcgcgag gtttacgacc gtaaacgact tcccctcggc cggagtcgcg ctcgtaatca
     2401 ccaccgtgtg gatcggctgc attttctgca agtggttgag gcgcgtccgc agcgtgcgga
     2461 attcttcact gtgcgcgtca tgcgggcggc tcgggtcgac cagataagag tccggcgagg
     2521 ggttgtaggg cacttcctgc acctgctcca gcagttccag gaagtccctg ccgggcatgc
     2581 gcccgctgga cggccgcttc gccctcggac ggacgtcccc tgcctgctcg gcgcggcgca
     2641 acgcgtcatg tacccggctc attcatcccc ccaggattca tcttcatgcc tgttttgtgt
     2701 agtagtagta gaccgatgtg atcatcacca gaaagccgac aatgcaagcg ctcgaccatg
     2761 ccagccaggt caatcgccgc ttgcggcgca ccaccagatc attctcgagc agcggcacgc
     2821 tcccaagcac cgtcagaccg gtgtacgccc gcacgtcctt gagattcttg agcgacgtat
     2881 cccggatttc acgcatcccc gccaggaaca ggccggcgac gaggccgagc caggtgccaa
     2941 tggcgataat cagccagcgc ttcggttccg ttggcgtctg tggcagcgtg gccgggtcga
     3001 gcagttcgag cgtctcgccc tgtttgcggc tctcgaggtc cgtcgcgatc tccgagttcg
     3061 cccgcttctg gctcagctcc tcatagcggt tccgcgccag cgcatagtcc cgcgtgagtt
     3121 ctgcgtattc gcgctcgccc atcggaatcg cttctacgcg gtcctggaac gagcggatgg
     3181 ccttatccag cctcccctgg tccttcacgt actgctcgat ctccatgtcc ttggcgcgga
     3241 tgaagctctg ctgccgctgg atcgccgcct ccagttccgc tccctcgcgc gtcctgatag
     3301 gcgccgtctc cttcgtcttc ggcacggcgg cttcccgttt gacttcctcg tccaggagcg
     3361 catcgcggtt tttcttaagt accgcaagct gtgcctgtac gcgcctcacg tccggatgcg
     3421 tgtctctgta ctgctggcgt agtccggaaa gcatcgtctc gaggtccaga atctgcttct
     3481 cggtctgcat cagacgctcc gacttcgcag ccgtttcggc cgtgtttccg ggtgtggtcg
     3541 ggacccgctg ttgctgatcc cggagagtac gaagctgtga ctcgagcaat agtttttcct
     3601 gcgtcgcccg gttgatcgag gagctcatcg ccgagagttg cgtctcgagc gtccgcaact
     3661 gttgcaggtt tgagtcccgc tgttcgggca gccggcccat gttgcgcatc ttgaactccg
     3721 tgagccggtt ttcgatctcg tcgagctgtc tcttcgcctc cgccagcttg tcgttcagga
     3781 aatccgtcgt catcaccgac tggctggccc gcgtggtgat gctctcatcg atgaaccggc
     3841 ttaccagttc ctgcaccacc ttctgcgcaa ggtacctgtt ctcgtaggca aactcgatcc
     3901 ggtacgccga ggaggccggt cggccctgca tgccttcgtc cctcaggctg aacaccggat
     3961 tgatcttgat gtctttactc attctttcca ggacgtcttc catcggcacg cggctgcgga
     4021 gcccggggta caatccgtgc gcgttcacca tgtccgtcaa ccgagcccgg ctggtaatgg
     4081 actgcgccat cgccgaaaag cgttgcgtca tctcgatgtt cacgttcgac gcaacgtact
     4141 tctccggcac ctgcggtgga acgacgcgaa tcacggccat cgaaacgtag gtatccggcc
     4201 agaggaacgc caccacaacc gcaatcacca ggcccgcaaa agcgggaccg gcaatccagc
     4261 ccctgtgccg ccggaccacg tcgacgtaat cctcaacgtc catcgaccgc cgctggacat
     4321 tgtaggattc gggcgtcggc tgcataagtt actccccctg gctaagttgc gatcgcggac
     4381 tcaaggaaca ataatgtggt cgccgctctc cagcagcatg ttctgctgca tattcctgcc
     4441 ccgcactacc tccttgtagt tgaaggtgta ccgcttgggg cctcgcagta tcagaatctt
     4501 cttccggtcg gcgtattccc gaaatccgcc cgcgaccgcg agtgcttcca gaatggtggt
     4561 gggaactacc agcgggtagg agccgggccg catcacctct ccgctgatga aatatttctt
     4621 gctgttgacc gaagccacct ggaccatcac ttcggggttt gtcacgtact tcgaaaaccc
     4681 ctgcgtgatg ctctgcgcca gcttctcggg tgtcaacccg ccggcttgca gatcgccgag
     4741 aagcggcagt gagatctttc cgtccggccg cactcccacc ggacccgaaa gctccggctc
     4801 ccgccagaca cgaatcatca gaatatcctc ggcgccgatc acgtaattct ttggatcgat
     4861 gggggccccg accgcctttc gaagcgcttc ttcctccggc gtcaccggcg cgcccggcga
     4921 ctgctgttgc tgagacgatt gctgcttctc ctgggccggc gttggcggca caaatgaact
     4981 catacacaat attaatattc ctgcgaatat cctaaggccc gaattccccc gctttaacat
     5041 gaaagtaacg tctattctta tcggcgcgca gcctatattg tacaacagct tccgcgattt
     5101 cgacccggaa ccgcgaccgc agggagcggt caaaattcct cctccggcgg aacgcccaat
     5161 tgcttcagct tccggtacac catcgccacc ggcaacccca ccacgttgaa ataacacccc
     5221 tcaatccggt caatgaactt cgacgccagg ccctgaatcg cgtacgcccc tgccttgtcc
     5281 atcgattcgc cgctcgccgc gtagtcgagg atctcctcct ccgacagcgc cacgaaccgc
     5341 accaccgtcg actcgacgtc cacctccgtc ccgcccgtcg cccgcacgca cacgccagtc
     5401 atcacgacgt gctcccggcc cgacagtttc cgcagcatcc gctcggcgtc gctcgtgtcc
     5461 tccggcttgc cgagaacttc cccgtccacc accaccaccg tgtccgcccc cagcaccacg
     5521 tctcccggcc gggcggccgt cgcctccact ttctcgcgcg ccaggcgccg cacgtgttcc
     5581 tccgggcttt cgtcctccaa ccggctttca tccacctctg ccggccgaac cagaaacggg
     5641 atccccgccc gggtaagtac ctcttggcgg cggggcgact gcgatgcaag caccagcata
     5701 tccttagatc ctacacgcac ccggacctca ctggcccgcg tgctagaatg ttttcttcca
     5761 cccgccatgg agcgtgccgc ccggctcatc gcgcgtctca aacttccgtc gggatcagtg
     5821 accccggaag atctcgcgcg cgcggcatgg ccgctcgccg tcggcaaaac tgtcgccgcc
     5881 cacaccaggg tcttctctat ggtccgaaac tcgctcatcg tcgaagtcga ggaccacgtc
     5941 tggcggcggc agttgttcgt gatgtccggg cagatcctgc gaaaaatcga agaggtgctc
     6001 ggctcgccgc tcgtcggcgc cctcgagttc cgcgtcggtc tttcgaagcg tccgccccag
     6061 cgcgccgaac agccgcgccc ctcggccgac gaggccgacg gcatcgaaga cccagttctc
     6121 cggcgtattt acatcgcgaa gcggaaaagg gagaccgctt gaaaatcacc gaagagcaag
     6181 tccgctacgt cgccgatctc gccaacctgc gcctcaccgc cgacgagatc cgccggctac
     6241 gcgccgacct cgacgaaatc cttgcccaca tggataagct caacgaactc gacaccgccg
     6301 gcgttgagcc catggcccag gtgctctacg aggccgaaga gactgctact ctgcgcgacg
     6361 acatcgagcg tcccgtgcta ggcacggacg tcgctctggc aaacgcgccc cagtccggcg
     6421 ccggttattt caaggtcccg aaggtgatcg aaaggtagcc catgaacatc gcatcgctca
     6481 ccatcgggaa cgtccgcgag gggctgctgt cacggcaatt cagcgctgag gagcttgcgg
     6541 ccgcggccct tcgccacgcc gagaccgaaa atccggccac caacgcctac ctccactttt
     6601 gtcctgaacg tgcgctcgca tccgcccgcc gcgtcgacgc ccggctcgcc cggggcgagg
     6661 accctggccc gctcgccggc gtccccatcg ctgtcaagga cgtcatcctg acgaaaggcg
     6721 tgcgctccac cgccggttcc cgcctgctcg aacactacat cccgccctac gacgccaccg
     6781 cggtcgtccg cctggaggag gccggcggcg tcatcctcgg caagacgaac tgcgacgagt
     6841 tcgccatggg ttcctccaac gagaactccg ccttcggccc cgtgcggaac ccggtggacc
     6901 ccgaacgcgt tcccggcggc tccagcggcg gctccggcgc tgcggtcgcg cagggcacgg
     6961 ccgtggtctc gctgggctcc gacaccggcg gctccattcg ccagcctgcc tcgttctgcg
     7021 gagtagtcgg catcacaccc acctacggcc gcgtctcgcg ctacgggctc atcgccttcg
     7081 ccagttcgct cgaccacatc ggcccgttcg cccgcaacac ccgtgatgcc gcgcgcttgc
     7141 tccaggtgat cgccggccgc gaccagatgg attccacctc ggccttcgct ccggtaccgg
     7201 attacaccgc tctgctcgac ggcaacgtga agggcctcaa gatcggcctt ccgcgtgaat
     7261 acttccaagg cctcgccagc gaaaccggcg atctcatcgc ccgcggagtg gacgtgctga
     7321 agcgcctcgg ttgccaggtg cgcgaaatca gcctcccggc cacggattac gccgttgcct
     7381 gctactacat catctgcacg gccgaggcga gttccaacct cgcgcgctac gacggcgtcc
     7441 gctacaccac ccgctcgaag gacgctggca cgctcatcga gatgtaccgc gccacccgcg
     7501 gcgagggctt cggcgccgag tgcaagcgcc gcatcatgct cggcacttac gtgctgagcg
     7561 ccggctacta tgacgcctac tacctgaaag cgcagaaagt ccgcgcgctg atcgcccgcg
     7621 atttcgccgc ggcgttccgg gatgtggatg cgatcgtagc cccagtctct ccgttccccg
     7681 ccttcaagct gggcgaaaag atcgacgatc cg
//
LOCUS       DATIFQ010000359        70271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_118056, whole genome shotgun sequence.
ACCESSION   DATIFQ010000359 DATIFQ010000000
VERSION     DATIFQ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 70271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70271
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118056"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VLE22_17090"
     CDS             <1..1051
                     /locus_tag="VLE22_17090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB16173.1"
                     /translation="HLAITHRCNLRCRFCHIRDSVFEELDTDGMKRVIDRLDEMGVGV
                     VSISGGGEPLLRPDFAEIVDYAAGKGMYTKLTSNGTLPLSRYERLLRSRVREIGVSLD
                     GVQGNDLPHSHVGPRILDSIRYLHDHLPPQKQLTINVTVSGTNRHQVEEIVAFCAREF
                     PRAKVWLNPVMAGQGALRTAEAVSTPPDYLRRCRSPNLLSAKFYIEGVEKQFRRGHFD
                     WGCRAGATFFDIKPNGDFWLCQDQPSRTPLNILDPAFRRKLRASDFRYRKECTGCTYS
                     CYYVTQNGLSPRRWPDMALLWWTSNTMHGDPCRQAAGRYGWLAGLGMYLLLKLRTGVS
                     SMAAAALPVLPLMGA"
     gene            complement(1100..1176)
                     /locus_tag="VLE22_17095"
     tRNA            complement(1100..1176)
                     /locus_tag="VLE22_17095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1140..1142),aa:Arg,seq:tcg)
     gene            1314..2249
                     /gene="mdh"
                     /locus_tag="VLE22_17100"
     CDS             1314..2249
                     /gene="mdh"
                     /locus_tag="VLE22_17100"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HSB16174.1"
                     /translation="MRKKVTVVGAGNVGANCALRIADKELADVVLVDVVEGVPQGKGL
                     DLLQSGPVQGYDVTVTGANNYETTAESDIVVITAGFPRKPGMSRDDLLIANYEIVKTA
                     TEQAAKYSPNSIIIIVTNPLDAMCWTAYTVSKFSKNRVVGMAGILDTARFRTFIAQEL
                     SVSVENITALVLGGHGDTMVPLVRLSSVSGIPLTELLDQSTLDRLVQRTRDGGAEIVR
                     YLKTGSAYYAPSAAAVEMVESILKDKKEVLPCAAYLEGEYGINGLFVGVPVKLGAGGI
                     EKIYEVKLSAEEKAMLNKSAAAVQELIEILKPKLS"
     gene            complement(2242..3528)
                     /gene="eno"
                     /locus_tag="VLE22_17105"
     CDS             complement(2242..3528)
                     /gene="eno"
                     /locus_tag="VLE22_17105"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSB16175.1"
                     /translation="MKLTVRRVRALEILDSRGNPTLSVDVELSDGTRGAAQVPSGAST
                     GRHEAVELRDGDPARYGGKGVYRAVASVEKVLAPALAGSDAEDQAGLDRLMISLDGTE
                     AKSRLGANAILGVSCAVARAAASARGLELWEYLCGGRTPRLPVPMINIISGGIHAGHN
                     FEFQDFMIIPHGFQSYSAALEAAVRVHRATQEVLEAAGRPVSGLADEGGWGPLLASNE
                     MALLVLLRAIELAEFRAGDQVLIAIDVAATHFLRDGAYELRSEGRKLTGAEMIELLDG
                     WSRRYPVISIEDGLAEDDWDGWKSLNAALGSRIQLVGDDFFTTNPARVERGIRERAAN
                     AVLVKMNQIGTLTETFQVIDMAREAGFRAVISARSGETEDSFLADLAVASGAGQIKVG
                     SVARSERLAKYNRLLEIERDHAARCPWKPAACLPLN"
     gene            complement(3525..4523)
                     /locus_tag="VLE22_17110"
     CDS             complement(3525..4523)
                     /locus_tag="VLE22_17110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HSB16176.1"
                     /translation="MCGLFELDALNAARYLQSARGLDPELVKITTLGGGISNTVLLVD
                     TPAAPFVLKQALGKLRVEQDWFSDRHRIFRECEALRRLAPHLEPGAVPDVLFEDRENC
                     LFAMSAAPPSAQNWKSLLLGRLLQAEIAARVARMLAGMIRLGWRNPEFESIFGDQTVF
                     DQLRLDPYYRSTASRHPDLRPHFDRLVEDCRGFRLTLVHGDWSPKNFLVDGSSAMAID
                     FEVIHYGDPAFDAAFLLNHLLLKSFHLPDVAAEFAALADVFWRTLLDGYPAEDAGFEE
                     RTIRHLGGLLLARIDGKSPAEYIRDSGARDQIRSFARRLILTPPPTVQEIFRRLER"
     gene            complement(4562..5749)
                     /locus_tag="VLE22_17115"
     CDS             complement(4562..5749)
                     /locus_tag="VLE22_17115"
                     /inference="COORDINATES: protein motif:HMM:NF012369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB16177.1"
                     /translation="MAGGNFKRALLVCPDRSMADELGAVLSRETIVGEILDCKTYLAS
                     RPLAELLVSGPPDLCFLDVCSTPDQADRILAQLSDQPTKLPVIALLGRNEPERILRHL
                     RRGAAGFLLRPFSKEQLKAALEKVAALFPQGRGVANGAGKVVSVVPAKGSCGATTIAS
                     NLACQAKQLGVSKVLLVDLDPLTSTLSFLWKLKPQYSFADALSHVNELDADLWKALVS
                     GYRGLDVLPSPENPSDCAGDAPQLAELIEYCRGVYDLVFLDLGNPYGAWNLKAARVAD
                     EVLLVVTTDVPAVYGAQRTLACLERNGIARHRVRLLVNRFRKDYGLEPGDVESALGVE
                     ITQLLPYDREAIDSALIEARPVTGNSQFGKSLAELAARLADRETAARKPQAGGLLSLF
                     SRA"
     gene            5988..6530
                     /locus_tag="VLE22_17120"
     CDS             5988..6530
                     /locus_tag="VLE22_17120"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB16178.1"
                     /translation="MRSAHSLASGAEVIRTERPKVALLDKAFGIHGLVQWMADLGPML
                     RETSVAVWGVSINETEALRFIQAGAKGVIRKTADTCVLLNCLRTIAAGSTWMEDSIFR
                     DFPRLVRCPHSDLTPRELQVLSLVEHGMKNQEIARELGIRPGTVKIHLKHIFEKTGVR
                     GRHGLALSGLRERGALSLPS"
     gene            6596..8272
                     /locus_tag="VLE22_17125"
     CDS             6596..8272
                     /locus_tag="VLE22_17125"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HSB16179.1"
                     /translation="MQRVLRTVILALWAATIFGNSASLLDILSGELERNFRVLHEKGD
                     PPAYYIAYAVTDQESHNVGATLGALQVASHASSRWLDVTVRVGSPELDNYHRLSGDRF
                     AFSSAVRLPLENVPAAICQRLWMDTDGTYRRAAERLIKVRTAAQVKAVEADSAADFSK
                     EEPAIYEDAPQPLEFSPQDWQDRVRKWSAAFGRYPRILASSVSVSAQLETKYLVTSEG
                     SRIEQGKGLARIALSAQTKAPDGMDLTSEESFESETVRGLPGDKEVLGAVERVAKELT
                     ALLSAPDAEPFVGPAILSGRAAGVFFHEIFGHRIEGHRQNDETEGQTFAKAVGTKVLP
                     DFLSVFSDPTIRKLHGVDLSGHYLYDDEGVKARRVTVVESGILRNFLLSRSPMPGFDH
                     SNGHGRRQPGFDVVSRQSTLIVESSKTVDEARLRGLLIEEIRRQKKPYGLYFEKVTGG
                     YTQTSRRGLQAYTVVPIIVYRVYADGRPDELVRGADIVGTPLASFSKILAASDQPGVF
                     NGYCGAESGSLPVSAVAPSLLVSEIEIQKKEKGQDRPPLLPAPSGEWRSH"
     gene            8272..10065
                     /locus_tag="VLE22_17130"
     CDS             8272..10065
                     /locus_tag="VLE22_17130"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HSB16180.1"
                     /translation="MPTAAVRQRSSAPAALLVTLVLPLAAGQGTPPREDVILRAMHDE
                     LERARELTVETLESPYYVEYALHDGETLIIDASLGALAGVRQARFRQPTVQVRVGSPQ
                     FDNTNHVLSDYMFGARYDAGQTPLDNSYDLLRRYFWLATDRAYKSALEVIARKRAALR
                     NFNSGEQPPDFAKAEPSKIILRVETEQLDVESWKARLRSLSAVFTGYPAVVSSGVQLQ
                     ANQGAFYFANSEGAEVRIPETLIFLEVRARALAPDGMMLRDSMVFQSRSVSGLPTDAE
                     MQRGIEEVGDSLKALIEAAPANTYTGPVLFEARAAAQMFAEVLGRSLGLRRKPVSEPG
                     RPLPSPPNELEGRIGSQILPSWMDVVDDPAQAEWRGRPLFGHYLVDLEGVTPKPLSLV
                     EKGVLKTYLLTRQPVGGFSESNGRARLPGGFGVKAPGFGNLFVRASQTVAQAELKRQM
                     LELCRLRDKPYGILIRKMDFPSSASLEEFRRLAPRMSQVASGARPVSLPVLVYRVYPD
                     GREELVRGLRFRDLAVRSLRDIVAASDEHHVFEFLDNNAPFALMGAGGFAAESAVVAP
                     AVLFDELQLDRAEEQIPKPPVVPPPPLSGTR"
     gene            10068..12389
                     /locus_tag="VLE22_17135"
     CDS             10068..12389
                     /locus_tag="VLE22_17135"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HSB16181.1"
                     /translation="MGILNRRVFREIASGALLGGVLFTFVLFLQRIGRLFELLVRSSA
                     SPSTVAELFALVLPPTLPFTVPIGVLVGILLGLSRMSSDGEITAMRSAGIPSRRLLSP
                     VILFAAFATAITAAASLWLTPWSIRETYRIVNRLAAAQLTAEIQPRVFEEQFPNKILY
                     VADVISGPKVRWRNVFMADLTPPAERRAGAGETGEGPRITVAREAIAVPDVARNTIQL
                     SMIDGSTHEAAKDPAEYYNSAFPRGDQVLDASPPDERRARPFQETDTPPLFRIAKDSV
                     DARIELHQRLALPPACILLALVGLPLGVSVRKAGKSSALVLTVFLAFLYYMGLISLIG
                     LAREKTLPVELAVWTPNALFALGGLLLAWRLEKPGDRDVVGAIRGRLGSAWRWLRERF
                     VHTKNGLRSDATAGRMPLLPQVLDTYILSSFLVFLTLSLTSFVLMTHVFTFFELLGDI
                     IKNHIPMTRVLTYLVFLTPKLIYDSTPVSVMVAVLVTFGVLTKHNEVIAFRACGVSIH
                     RLAIPIMLASTLLSVTLFAFDYYYIPHSNRIQDAIRAEIKGRPVQTYLRPDRKWIFGQ
                     GPRIYYYRYFDPVANMMAGVSVFYLEPGSFRLRRHITAEQARWSPTLRTWVFENGWSR
                     DIRGIQESNFQQFQATTFPELNEPPGWFLKEVKQDKQMNFQELSAYIGELRQSGFDTV
                     RLSVQFHKKFSVPLFALILAMISFPFAFVAGNRGALAGVGVSFGIAIAYWAVSQLFEQ
                     IGNVNQLPATLAAWAPDAVFSLAGMYLIARMRT"
     gene            12583..13263
                     /locus_tag="VLE22_17140"
     CDS             12583..13263
                     /locus_tag="VLE22_17140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSB16182.1"
                     /translation="MTRALMFLLVGLLAAGSAAFAAPIYFNDFQSSVGAGWSNTTTST
                     TPAGCTTCTTYLGQFGNQSVTLTLTGLPTSTQLTISFDEFIIRSWDGNGEQGFGPDTA
                     AFSIGGANTLTTTFANGLVGNTQNYPTAGSAAQTGAAQVNTLGYTFIGVPRDSIYALS
                     FNWFNTSSTQTFTWSASGLQELADESWGIDNVSVSGPDEAIPEPGTALLLGSGVLAFL
                     LYRRRRSA"
     gene            13460..14485
                     /locus_tag="VLE22_17145"
     CDS             13460..14485
                     /locus_tag="VLE22_17145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB16183.1"
                     /translation="MRKVPLAESLKIIGRGSLNWIAERPIVVSFEVTDACTCYCRHCD
                     HGGPRDGSRNLKASDFRRYVDALRPCVVQVSGGEPLMRRDIVDIVRSIKSDGRIPYTI
                     LVSNWSEMTEEIYLKLHKAGVDQFSVSLDFPDNRHDDFRGYPGLYSHLSDLVPRLAKY
                     GYDDIVLNSCITSVNVREINAMADKAQEWGVNICYSAYSARRTGCRDYFLNTSEELET
                     LNQELNRVESRRDESNWIVNATTTLEATRRYFENRGAPGCKAGLRFLVVTADGQLQPC
                     SMQFKRYPLQEQARMVQEFTANNTCDECYVSIRSYLDKSFPQLLWENVAGFLSFKAGT
                     RARSSGN"
     gene            complement(14493..15722)
                     /locus_tag="VLE22_17150"
     CDS             complement(14493..15722)
                     /locus_tag="VLE22_17150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB16184.1"
                     /translation="MARKLKDYEEIVGPEVIEEIQVVADRVRGRCLQNINSTPVGGGV
                     AEILTRLVPLLREVGVDARWDVIKGDQEFFEVTKAFHNALHGDPQEVTNRMFEVFRAN
                     TEVNLREMEFTGDVVLIHDPQPAGLILRKPEIGRKWTWRCHIDVSAPNPEVWAFLRPY
                     VEQYNAAIFSMPDFAQELPIPQYMVAPSIDPLSDKNRDLEPGCIDTVLAKFGLDPDRP
                     IVTQISRFDRLKDPLGVIAAYRMTRKRVDCQLVLAGGGAPDDPEGAQVLQEVQERAGE
                     DPDIHVLLLPPFSDLEINALVRASTVVMQKSIREGFGLTVSEALWKKKPVIGGAVGGI
                     KQQIINGITGFLIHSPEGAAQRTVQLLRDPELCRWLGENGHQHVKQNFLLTRHLKDYM
                     LMMLALDYPDEHIVFLG"
     gene            complement(15724..16368)
                     /locus_tag="VLE22_17155"
     CDS             complement(15724..16368)
                     /locus_tag="VLE22_17155"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HSB16185.1"
                     /translation="MSPFTFYTERRLVRLTSRRARTLDELAHHLSDVSGASVFYHTHH
                     QFLSHHFEKPVYHNEFANWVGDALQYRRLSEELTAVDLLACTTVRQIREELLARIESQ
                     LRADAGDTRSCRPGEEFHFCESQSFVMPTGLVADDVPEFFSTLRRVSNVSLFFHFFQA
                     RLRLGEPTNDFSQWLCYRGQEALAREIDQLDPYGMTLDELRDEMIRCGSRHEVA"
     gene            complement(16374..17192)
                     /gene="otsB"
                     /locus_tag="VLE22_17160"
     CDS             complement(16374..17192)
                     /gene="otsB"
                     /locus_tag="VLE22_17160"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HSB16186.1"
                     /translation="MLATLKPPSTKPDVAHPLWERIDEIRARIQNSGRLLLLLDFDGT
                     LAPIVADPSAARLPLPVRDLLVSLVGSRSTLPAVISGRALDDVRTRVGIPGLTYAGNH
                     GLEIEGPGFRFVQPEAERLRPLIAGACMSLSRRLRGVPGVEVEDKGLTAAVHYRNAPE
                     SQNRVIEAVWDEVVRRDRGLALRMGKMICEVRPAVDWNKGSAALWLCSRLADGETVLP
                     ICAGDDQTDEDAFAALPGGVTVKVGDPLGTAAAYYVAGPDDVGQFLKWIGETCQ"
     gene            complement(17161..18678)
                     /locus_tag="VLE22_17165"
     CDS             complement(17161..18678)
                     /locus_tag="VLE22_17165"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HSB16187.1"
                     /translation="MWTKQDLHELIALRLQGLQFVVVSNREPYLHRLSGREIECIQPA
                     SGMTAALDPIMRAAGGVWIAHGAGNADRRTVDANNHVAVPPGNSQKYTLRRVWLTKEQ
                     ENRYYYGLSNEGLWPLCHIAFTRPVFRPEDWRAYREVNEIFARAVLDEIGDRPTFVFI
                     QDYHFGLLPRMLKDSGLPNLFVAQFWHIPWPNREVFRAFPWGEELLDGLLGNDLLGFH
                     IRYHCQNFLDTVDRTLEARIDPELYEITRRERTTAVRPFPISVDFESHSASASSEQVE
                     SEMERWRQELGLGDELVGIGLDRIDYTKGIPDRLQAIDRLLDEYPEYRGKLVFIQVGV
                     PSRVDIGRYRSLGYEIEALADEINRKWSTDSWRPVILLSRHVGSVRMMALHRLARFCF
                     VSSLHDGMNLVAKEFVASRNDDDGVLILSRFAGAAYELTDALLVNPFSQEEMAEAVRQ
                     ALEMPTEERRRRMQRLRGVVSENNIYRWAGKILSTILKFDFPENADVSHFEAAVH"
     gene            complement(18820..19599)
                     /locus_tag="VLE22_17170"
     CDS             complement(18820..19599)
                     /locus_tag="VLE22_17170"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB16188.1"
                     /translation="MNVPRDVLQREVEYHERLYSGFAQKHFSKAAVRELRAHLVGRML
                     SMCPVGLESRVLSLGCGIGDTELLLAPHVGELIGVDLSPAGIRQAQADAATAGLTNAR
                     FLHGTIDDVEGEFDFIYAIFFLHHLPDESLPGTARTIAGRLRPGGWFYSLDPNRYRLS
                     GALGKLLVPHLMRKYQTPDERELPPSPTADLFRQAGLDARHEYYDMLSTPLGGLLPGW
                     RSGYRVVRAIDEVFLRIPLFRRFASSFEVIARKSTERSPMR"
     gene            complement(19618..20964)
                     /locus_tag="VLE22_17175"
     CDS             complement(19618..20964)
                     /locus_tag="VLE22_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16189.1"
                     /translation="MKQASDDTGHRPARFTLLLFACSLVVYNLNFQRIASGDTLPASL
                     LPFSILLDRTVNLDFFYPAVREHVPQHLRALPIVSGHAYSEYPIALPILLTPLYVPAA
                     VYVTAAGWSPAQVMVLAGVLEKLTASLIAAGSVALFYLLAVRLADEKRARWLAILYGF
                     GTATWTISSQALWQHGGSQVAIILALFFLDRLRECADDRQAALLTGLFAGLSAAIRPT
                     NILFVAAAFTGLLASSRRVRLMAAFWLFPVLAGVVTVAYNVGVFGAVQGAPALSFDGN
                     LWRGLAGVLFSPARGLFVYSPVLLFFVAGVFFLFRRRKWPAQPVYLAALVFAVSQVVL
                     IATWRVWWGGHCYGPRLLSDALPAFVVLMAPATETIFRSRTLRAVFVVLLTFSIFVQA
                     VGAFCYSASMWDEKPVNIGERPSRLWDWTDNPISRSVKAGPRLGPIVDILDRLRSG"
     gene            complement(20945..21922)
                     /locus_tag="VLE22_17180"
     CDS             complement(20945..21922)
                     /locus_tag="VLE22_17180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB16190.1"
                     /translation="MIFKGLVSTDHPVLAHLIPIRRCNLSCAYCNEYDDVSKPLPTET
                     MLQRVDRLRELGTTVITLSGGEPLLHPDLDEIIAHIHRRGIIAGLITNGYLLTAARIH
                     RLNRAGLDHLQISIDNVTPDDVSKKSLKVLDKKLQLLAGHAEFHVNINSVVGGGIRNP
                     QDALTVGRRAVELGFTSTVGIIHDGSGQLRPLGDQEREVFLAMKGLEKSSYSQINYFQ
                     NNIANGRPSNWRCRAGSRYLYVCEDGLVHYCSQQRGYPAKPLAEYTKDDIRREFLTEK
                     PCAPFCTVSCVHQVSYMDFWRAPQTIAATPATESESRESELVHIETGIR"
     gene            complement(22087..22899)
                     /locus_tag="VLE22_17185"
     CDS             complement(22087..22899)
                     /locus_tag="VLE22_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16191.1"
                     /translation="MLRALRLFLITAMAFTAVRAGKEELTPAETDEIINKFAAKEAEF
                     ALARENYTYRQTARIQELDDSGRVRGRHEMVSDIIFSSDGKRTERVVRAPVSTLQNLL
                     LTPEDEQDLRNVQPFVLTTKELPKYDIRYLGKQMADEIPCYLFAVKPKKMEQGQRYFS
                     GQIWVDDRDLQIVKTYGRGVGLLKRGSDNQFPKFETYREQIDGKYWFPTYTISNDVLQ
                     FESGMRVPIRMTVRYEDYKQFKSEVQIKYGEEVKPTPPAPPTPPAPEAAKKP"
     gene            complement(22934..24106)
                     /locus_tag="VLE22_17190"
     CDS             complement(22934..24106)
                     /locus_tag="VLE22_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HSB16192.1"
                     /translation="MSTVAEGPARPLSQLSEDEKLFQASVRQFAKEQVSPHVRAMDEA
                     GVFRKEILREFFELGLMGIEIPEEHGGQGGTFFQSVLAVEEIAAVDPAAAVIVDVQNT
                     LVNNALLRWGNDEQRKRYLPKLAADTVGAYALSEAGAGSDAFALAARAADQGDHFLLS
                     GRKLWTTNGAEAGLFLVFANANPEAGHRGITCFLVERNSPGFEVGKKEDKLGIRASST
                     CELILDNCRVPKSNLMGELGKGYKIAIETLNEGRIGISAQMIGLARGALEQALSYARQ
                     RKQFGKPIAEFQGIQFDLARMATELEAARLLTYNAARLRDAGMPFLTEAAMAKYYSSE
                     IAETVASKAVEVFGGVGFTKDCPAEKFYRDAKIGRIYEGTSNMQLMTIARQILGKP"
     gene            24166..24345
                     /locus_tag="VLE22_17195"
     CDS             24166..24345
                     /locus_tag="VLE22_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16193.1"
                     /translation="MKNWKLIAQGLDVAIPEADLPKLSQTLEAMDTAFRPLAEAIPDE
                     MEPAVIFHIQPEEQV"
     gene            24342..25730
                     /locus_tag="VLE22_17200"
     CDS             24342..25730
                     /locus_tag="VLE22_17200"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSB16194.1"
                     /translation="MTILEAAAAIRAKKVTSAELTAESLSSIARLQPRLNAFITVAEE
                     RARERARRADEELKRGMDRGPLHGIPIAVKDVFCMRGMRTTCGSKLFENHVPDRDAAA
                     VERLEAAGAVITGKANMHELAYGITSSNPHFGFVRNARNPEHIPGGSSGGSGSAVAAG
                     MVFMAMGSDTGGSIRIPASFCGTVGIKPTFGRVSRYGVMPLDFSLDHMGPLTRTVRDA
                     AVCLGAIAGHDPRDETSSRQPVPEFLPPRQVSIQGLRIGIPENFYFERVDPEVEAAVR
                     RMASAAANLGATLVPVRVPDIRELNVIARVILLAEASAVMEKHLSRRENFGADVLSLL
                     DQGRLIAATDYVNAQRLRRVKQQEFRRIWRHADCLLTPCTPTPAPKIGQTAITIAGEA
                     EDARLASTRLVRAINLLGLPALALPCGVHTLGLPMGTQLIGRPFTEPLLFRVGAALED
                     VTGFDKRVPPEC"
     gene            25796..27112
                     /locus_tag="VLE22_17205"
     CDS             25796..27112
                     /locus_tag="VLE22_17205"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB16195.1"
                     /translation="MQLSRRNFLQTGAALAAAPATSRSYAANDRVQFGFIGVGARAHQ
                     LIPCLLDNPGAEIVAVVDAYRGRVGRAIERTSSRAKAVKDYREIIADKSIDAVVVATP
                     DHWHKQMVIEALRAGKDVYCEKPLTYRSSEGVEIIQAAKETGRMLQVGSQGMSSETER
                     KAKEIIKSGRLGKITMIRAAYNRNTPSGAWIYPIPPDASPETVNWEMFLGSAPKRPFN
                     LERFFRWRCFWDYSGGIATDLFVHLCTTIHFLMEAKMPSKAAALAQLYRWKETREVPD
                     TVNGVLEYPDGFSVNLSGTFNNEAAAESNFQILGTEGTLSIGFADLSFRPERVNDDNR
                     WIVDSWSQELEDAYYRDPKIQAREVPSSRKARILPGSESYREEGKDSTVAHFGHFLES
                     IRTREPYWQDATAGHHAAACAHMVNLSSREGRVVEWEFGKDDIKKA"
     gene            27170..28837
                     /locus_tag="VLE22_17210"
     CDS             27170..28837
                     /locus_tag="VLE22_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16196.1"
                     /translation="MYIRREVGRLLPHAAPSLSDFLFVSLLVWLFATGSGWEALLADG
                     DTGWHIRTGDYVLNTGSIPTTDLFSFSRPGEPWFAWEWLADVIFALLHDRWDLVGVVV
                     VAGVVIALTAVVLFLHMLWRGANVFAALAVVLPCVGAASIHFLARPHIFTLLLLAVSL
                     WVLDRDRRAPGVSVWILVPLSALWANLHGGFLALLACVGLLAAGCAAEALLVSGRCWT
                     AARRYAALTGACSLATLLNPFGYRLHLHIAQYLRSDWIKEAVDEFQSPKFRSESLLQF
                     ELLLLVSLLLAGILLSKRRIADALPVLFWAHLSLSSVRHVPVFVIVVGPLIARELTCL
                     WESWTATAPTRSLAGILNAVAQDLAPGFRRVSPWSVALVLAVLWLTPSSRWPRDFPAA
                     KFPVGLVGANRDRLASARIFTSDEWADYLIYRGWPRQRVFLDGRSDFYGPVLGKEYLR
                     LLEGRTGWRDSLKKHGFRFVLLPEDAPLASHLHACGDWRQTAADRQAVLFERISSAPV
                     RSLIKGRCSTDSSVLEASRHEIQREGHPATTTLSVPGGFRGGLARAR"
     gene            28978..29865
                     /gene="cpaB"
                     /locus_tag="VLE22_17215"
     CDS             28978..29865
                     /gene="cpaB"
                     /locus_tag="VLE22_17215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HSB16197.1"
                     /translation="MDRRFLTVLGVSLLFALVVASVFYQMNARFRGSARKRDKPKQAE
                     LVVAAASLPVGVTIKPADVKVVKVEGERFPKGAFAKVEDVLGRPVVSNILAEEPVMEG
                     RLAERGSGVGLAPVIPVGMRAVSVKVNEVVGVAGFVLPGMRVDVLATLRPQSGDGART
                     STVLQNILVLSAGQNIQTDSRGQAVNVPVVTLMVSPEQAETLTLAGNEGRIQLVLRNG
                     ADQTVQPTPGKDLTELYGHRKPQLAEVSEARPRPQPQPRVAPAPFPPPAPPEPIRDEI
                     VVIRGTQKSLEVIGETKSN"
     gene            29909..31249
                     /locus_tag="VLE22_17220"
     CDS             29909..31249
                     /locus_tag="VLE22_17220"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HSB16198.1"
                     /translation="MLGLLAAIGTASPAQPPAEDLALAIGGSAVIDYPEDIGRLATSN
                     PEVVDALAVTKREVLLQAKSHGMATIVIWSKSGRRDFYRATVSYNLEPVRKLLRDTFP
                     GEEIQVQAAKDSLSLTGRVSSQAVAERAGTLVTPLAKSVVNNLDVSSSAEKQILLRVR
                     FAELDHTASASFGASLISTGALNTPGRITTGQFSAPAPQKIFPPGHDLSGVTGVIPGG
                     NPGTFSNFSIGDALNIFAFRPDLNLGAVIQALRNRGLLQILAEPNLVTTNNKEASFLV
                     GGEFPVPIVQGGANAGAVTVQFREFGIRLAFLPAVTGHDTIKLHVKPEVSSLDFANGI
                     SVSGFSIPALSTRRMETNIELGLGQSFVIAGLIDDRATENLAKIPGLSQIPVLGALFK
                     SRSEKKTKTELIVLVTPEITGPVDASRVPSGPTMPLDFIPKVDRPPAAREEGRK"
     gene            31260..32468
                     /locus_tag="VLE22_17225"
     CDS             31260..32468
                     /locus_tag="VLE22_17225"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HSB16199.1"
                     /translation="MGRPGKWRSAELSAVLIAPDRELAAQFCRTLETTRAFQIVAELK
                     KYPSEQTLDIRLRQLRPDAVLVDLASNFETAAGLLRALAAFRPAVHVIGLHRTNDPDV
                     VLRALQLGASEFFHAPFDPAVQRDAVAGFLRLRRVEVQAGVPPGKVVVFSSAKPGSGA
                     SVLACQTALALREATGERILLADLDLTGGTVGFYLKVKCAHSLIDAVERCEHLEPAAW
                     AALTTTCDGLDVLPAPAVPREANPDNDRLRAVLDQSRHLYDWVLLDLPAIFHPVSLTA
                     LVECDSAFLVSTAELPSLHLARKAVTLLAQLGIGKERLNVLVNQAGRGDGMSGSDLEK
                     IFGCAVHSSFPSDYSSLHKVVTLGEAVAPDSNLGKAVGEFASRLAGLARSEKRRAGMV
                     FNSDPAFAGT"
     gene            32490..33851
                     /locus_tag="VLE22_17230"
     CDS             32490..33851
                     /locus_tag="VLE22_17230"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HSB16200.1"
                     /translation="MLTHGDTKASAEVTWEQRLLRNSARPKTALKPECQELKFILHRK
                     LLEKINLEALATIENQRVRGEVRQALITLMEGEPTLLSSLEKQQIVEEVLDEVFGLGP
                     LEALLQDTTISDILVNTHRAVFVERRGLLEPTNVSFRDDNHLLHIIDKIVSQVGRRID
                     ESTPMVDARLSDGSRVNAIIPPLAVDGPLLSIRRFSVDKLMPRDLVERKAMTPGMMDL
                     LEAAVKARLNIIIAGGTGAGKTTLLNALSAFIAPRERIVTIEDAAELQLKQPHVCRLE
                     TRPANLEGQGAVRQRELLVNSLRMRPDRVVVGEVRGAEALDMLQAMNTGHDGSLTTVH
                     ANSPRDAISRLEVMVSLANANMQLSAIRQQIASAVHLFVQAARLSDGSRRVVNVTEVT
                     GMEGEVITLQDIFVFEKRGLSPEGRVIGRFAATGIRPRFYEKFLAAGIQLRPDLFDEQ
                     LEI"
     gene            33863..34843
                     /locus_tag="VLE22_17235"
     CDS             33863..34843
                     /locus_tag="VLE22_17235"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSB16201.1"
                     /translation="MAFFWILFVTLWLLAIACWWCASRVLRNSDRSRIRGRLLGTPSS
                     STGKKEGSPAPVLIPTEDKDTGKIVLRLLDRFRLNVRLQELLEQSGLKWHVARLTHAC
                     LALALAGFAIGGWTAPPGHAEIPVLLACAGGALPLLLVSRKRRKRLRRFEEQFPDSLE
                     FVARSMRAGHAFSVSLEMLHREFQDPLASEFHRTFDEHNLGLPLDAALEKLARRVPLM
                     DVHFFVSAVLLQKRTGGNLAELLDKLAYVIRERFKLRGRIRAVSAHGRMTGAALSTIP
                     VGVAGMMFFVNREYVTFFANDPTGQWMAGGAVALQLIGYGIIRKIVSIEV"
     gene            34921..35841
                     /locus_tag="VLE22_17240"
     CDS             34921..35841
                     /locus_tag="VLE22_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSB16202.1"
                     /translation="MELLLTGILFVVVMGAVSAAGVRLWVRPKAAMERVTGAIVGAER
                     GVPVHPSLVFRDLIHRMGALLPASSREAGLTQRRMIRAGFRNPGAVKILYGAKIFLGA
                     VLPLAAGLAVAGWEGPSDTKMVVVAGAAAAGFMAPGYCLKLVVKRRQKRIRRGLPDAL
                     DLLVICVESGLGLDQAILQAAKELEISHPAISEEFALVNLELKAGKRRAEALRNLADR
                     TSVDELKKLVAVLIQADRFGTSISQSLRTHSDFMRVQARQQAEEKAAKLGVKLVFPIF
                     FCILPTLFVVTVGPVAVRIVRDLLPMMNNM"
     gene            36098..39058
                     /locus_tag="VLE22_17245"
     CDS             36098..39058
                     /locus_tag="VLE22_17245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16203.1"
                     /translation="MKREAWWSLVLCGCLAAPVCGGTFGKAVSIGGHASDLVLDETRG
                     ELYVANFTANRIEALAVPDGTLKHSINVAPQPGSLALSPDGRYLLVTHYANYQTPGMP
                     RNAITLLDLATGARQTFALSAPPLGAAFLINGRALIVTTTEFLSFEPVSGTVRLLDTV
                     EGVTATTLPAPPANFPPQIIAASVAASRDQQVVYGLTDTIRFRYDLLSGRVVSLGYTA
                     EPPLGPRVVSVSRDGAYYTAGWALFDRQGSVIAQFPGASGLLNVGSHAIDSDRGVIYA
                     QIPAEEGAPPVLMIADADNLTVRERVQLPENLAGRSVLSSDGSMMYSASDSGVLILPV
                     GNYSRARRLKTSSEEIFLRTGFCNRRVAVQGLWIEDESGATTDFTIASSHPAITVSPS
                     YGTTPARVEVRADPNALQNRSGTAVAWLDIRSSGAVNLPSRVRVLASLLEPDQRGTVI
                     HIPGRLVDILADPVRNRFYVLRQDRNEVLVFDAADYRRIAVLRTGNTPTQMAITADRR
                     LLLVGNDNSQIANVYDLDTLVQTAPVRFPPGHYPRSLAAAAGAILAASRVAGPNHTID
                     RVDLLTRTASELPTLGVYENKVDLHTVLVASGNGSSILVAEADGSLMLYDANAATFTI
                     SRKDYTSLEGAYAASNSGMFVVDNLLLNSSLVPVGRFEKGANLSSGFAFDGDSGLLTL
                     APPGGGAGVMERVDLSSAAAVRPTRIAEAPPEREPGFALTRTLAPLANRQAIVSLTTA
                     GFTVLSWNYDAATAPPRIDAVVNAADFSRPVAPGGLIAVFGEDLSPVNMATSEMPLPT
                     ALGDTCLTVNGAPVPMLFVSPGQVNAQLPFEAEGNVTLALHTPGGVSDNFPLSVSSTA
                     PSLFHSGSAGPLVDIATVVRAENNQLATQANPVHGGDALVIYLTGLGRVWPAVPAGSA
                     APYDPLAQVVIPPAVTLGGTELPVLYAGLTPGCAGVYQINVRVPHWAPAGSKVPLTID
                     QGDSSTTVTVRVVQ"
     gene            39138..39758
                     /locus_tag="VLE22_17250"
     CDS             39138..39758
                     /locus_tag="VLE22_17250"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB16204.1"
                     /translation="MPVVLLTAAAAGLAQTWEAGGSAGGSLSRNVGVSSQLGQASAGL
                     ASGAAFGAVLGQSIHRIVSGEIRYTFQPGEIQVASGGTRTSFRSQFHAIHYDLLFHTR
                     PEGSSVRPFFAAGGGGRIFKGTGKENAYQPLQEYALLTRTREWKPMASVGGGVKIAIS
                     PRMFLRVEFRDYISPFPKQVIAPADGSKISGWLHSVVPLVGISVPL"
     gene            39840..40766
                     /locus_tag="VLE22_17255"
     CDS             39840..40766
                     /locus_tag="VLE22_17255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HSB16205.1"
                     /translation="MRASFFWVISSLAVPLTLAGQNAPANQYVGGEVCRKCHPNVWLN
                     FYKNPHYKSVASGTEAPDKTGCEGCHGPGQAHVQASGGKATIRAFSVMEPKQVIDACL
                     ACHSRDLSKANIRRSSHTLAGVACSDCHSIHRSPIPKFLLAKEQRDVCYGCHAPVRSQ
                     FSMPFKHRVNEGFMQCTDCHNPHGAPAATWRMGARPRMVEHALANEEPCLKCHSDKRG
                     PFVFEHPSVRVDGCETCHNPHGSTNAKLLRRPVVFTVCLECHNGAGAFGRQGDGIRTQ
                     SASHNMADPRYQNCTACHVRIHGSNADPLFLR"
     gene            40778..42745
                     /locus_tag="VLE22_17260"
     CDS             40778..42745
                     /locus_tag="VLE22_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16206.1"
                     /translation="MRRTVFLILASLGPLAGQQTVTPTAATVGPPRGTEIGNYNVMNS
                     FETGYRFRSVGGDLGKYRSDVNFGNGIRLLGSRLTVNSKDGHGSLFDEIVLSTQGLGN
                     DPYQFSSLRIQKNGLYRYDLLWRLSDYFNPALPLAGGLHRMDTERRMQDHDLALLPQS
                     PVRLLLGYTRNSQDGAALTTVQSFSAGDVSPVFADIRRIRNEYRLGGEAELFGMKLHV
                     VRGWDDFKEDTPFTLAPGTAGTSSPFTTFRRNEPYHGTTPWWRVHLRGENKYWAAAGH
                     FTYAIGRRDFIFDEAAVGTDRFGSERNRQVLVAGDGRRPVTSGNVTLSFFPTDNLTIS
                     NHTAFHNTRMEGDSVYRELNNATASGPYLFFNFLGIRSITNTTDATLRVSKWLGLYGG
                     YYYSTRRIRSVEQVEIDGVPQRLPASQDNRLQSGLAGIRLNFARSLTVSLDAEVGRAD
                     RPFFPISERDYHGFNARVRYKSKSFALSASARTNYNTNSVSLSSHSSRGRNYSVDGSW
                     TPRDWFSVDGGYSKLHLDTASGIAYFVDFELIQGERSIYVSNIHAANLGVRFGLGKRA
                     DVYTGYSHVEDTGDGRVNPFIGSGARPDPFVPAQTFPLAFRSPLARLSVRLHGNLRWN
                     AGYQHYNYREDFFGRQSYRAHTGYTSVLWSF"
     gene            42749..43102
                     /locus_tag="VLE22_17265"
     CDS             42749..43102
                     /locus_tag="VLE22_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16207.1"
                     /translation="MELKLTLFNSITLMIMGFTLLTVIPLARGRLNRNWPFLYFPLVF
                     AYWRAFDGALNTWWICAGLVIALLLRFEIIGARSRAPLRWLSVIALAYVFLRGVDLLV
                     GGELFYFFSVGRPDA"
     gene            complement(43069..43461)
                     /locus_tag="VLE22_17270"
     CDS             complement(43069..43461)
                     /locus_tag="VLE22_17270"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSB16208.1"
                     /translation="MALSHGGKRHNPGFLALVNDSKSRVRQIDIDGYKRLVSSGEKFL
                     LVDTREESEWAAGHAAGALHLGKGIIERDIETHVPEKSTKMVLYCGGGYRSALAADAL
                     QKMGYTDVISLDGGWRAYQASGLPTEKK"
     gene            complement(43467..44723)
                     /gene="murA"
                     /locus_tag="VLE22_17275"
     CDS             complement(43467..44723)
                     /gene="murA"
                     /locus_tag="VLE22_17275"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HSB16209.1"
                     /translation="MDKFAIQGGVPLAGEIQVSGAKNSALPALAACLLTGEPVVLHRI
                     PHVRDIRTMQKLIERCGASTKANGGAVRVRAAEIVSPEAPYDLVKTMRASSLVLGPLA
                     ARCGRARVSLPGGCAIGARPIDLHLAGLEHLGARIRQEHGYVEAEAPNGLKGGFIHFD
                     RITVTGTEDLMMAAVLAEGETVLENAAREPEVADLAELLMRMGARIEGAGTSTIRIRG
                     VKRLHGAEHTIIPDRIEAGTFLIAGAITGGELLVKGCVPEHLGALIVKMRQAGVDVSQ
                     ESESSLRVRRTGRLTAVDATTEEHPGFATDLQAQYMALMTQAEGISFITETIFENRFM
                     HVLELVRMGASIRTEGRQAIVAGPRELTGAGVIASDLRASASLVLAALVARGETVIDR
                     VYHIDRGYEKIEQKLGKVGARIQRAA"
     gene            complement(44716..45609)
                     /gene="murQ"
                     /locus_tag="VLE22_17280"
     CDS             complement(44716..45609)
                     /gene="murQ"
                     /locus_tag="VLE22_17280"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HSB16210.1"
                     /translation="MLGKLLTEQPNPESEGIDALSTEGILQIFNAEDMKVAASVTSQI
                     PNVARLVDAAVAAIKQGGRLFYIGAGTSGRLGVLDASECPPTFNVSPGTVQGIIAGGE
                     RALAHSAEGIEDDPERGARDLKDRGFSAKDVLVGIAASGRTPYVLGAVAAARRIGAVT
                     AGISCTPDSELSRAVEIPIAPLPGPEVVTGSTRLKAGTATKLVLNMISTATMIRLGYV
                     FGNLMVNVQPTNEKLVNRARRIIAKAAEVDYEQAGALLEQSGGTVRTAIVMARLGVSR
                     EEAEKKLTACGGRVSEALRRG"
     gene            complement(45614..46450)
                     /locus_tag="VLE22_17285"
     CDS             complement(45614..46450)
                     /locus_tag="VLE22_17285"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB16211.1"
                     /translation="MYVCLNGSLLAGAKVGWPEFAHMAARLGYPGTEIYLSRAMSEGL
                     DSTRALLKTLKLKPAATDLLVEFRKDEATFQADLKKLEPTVKFSASIGAPGLLTWILP
                     SSDTPKAELRKIYKDRFQAVAAILAPSRMRLGLEFVSPMHLRKLHPHEFIWRMDEMLD
                     FCKECGPTVGLLLDAWHWHHAGATTRDIEDAGAERIVHVHVSDAPKLPPDQIRDNQRL
                     MPGEGVIDLAGFFQALKKVGYKYGVSPEVLGRLKKDTAPEDGARLGLQTTLVVMKKAG
                     AV"
     gene            complement(46460..47773)
                     /locus_tag="VLE22_17290"
     CDS             complement(46460..47773)
                     /locus_tag="VLE22_17290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSB16212.1"
                     /translation="MSDLAILGGKPVRNKPFPAWPQYDGRDRERLVAVLESRNWGGYP
                     FPNLLADEFARKFASHHGARYGCCLANGTIAILAALKAAGVGFGDEVIVPAYTWDGTA
                     TAVLDAGAIPIFVDVDPDTYCMDVEAARQALTPRTKALLPVHLAMRFAEMDPLLKLAE
                     ERDLIVIEDCAHVHGGQYKGRGAGSMGDIGCFSLQSSKLMTSGEGGIVITSRLDCFEL
                     VQSLVNCGRASVTDQFKKRIVGSNYRITEFQAAVLLGQLERLEELAAKRSRHATLLTK
                     MLAGIDGVRPLPAQPALTREAIYNYDFQYRPADRRVSRDLFVAALDAEGIPSDGRFYE
                     PVYRSDLFGAKPEDFPQLRIDREEPVNYRAFRCPVAERAAYEESVWLPQFLLLGDEAD
                     VEDIGRAVAKVMAGTDALVKADMSLAGVKSMSRADRPRVEKEKNY"
     gene            complement(47955..48278)
                     /locus_tag="VLE22_17295"
     CDS             complement(47955..48278)
                     /locus_tag="VLE22_17295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HSB16213.1"
                     /translation="MIQITETAVGKVKEILDMQEPKPAGLRIAVVGGGCSGFSYSMAF
                     ENSPGLLDKTYEFDGLKVFVDQASMLYLDGAKVDYVETLEGSGFKFDNPNVKSTCGCG
                     SSFNA"
     gene            48521..49186
                     /locus_tag="VLE22_17300"
     CDS             48521..49186
                     /locus_tag="VLE22_17300"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSB16214.1"
                     /translation="MSGISGALPLPLAGGRERAKGKVDYRNTEEGLLVRRVQKGDEMA
                     FRDVVERYQSKVFSIIYGILRNHNDAEDIAQQVFSKVYFSIRNFDFRSSLLTWIYKIT
                     VNECYDYLRKKRVRKLVYESDFSEEDTARLHSSAAVKDASPSFDTSLAQRDYVWKLLE
                     KVSEEDRRLMLLKEVEGHSVEELAAMTGMNENTIKVKLFRARQKLLKVAQRLARSTGT
                     ATA"
     gene            49313..49783
                     /locus_tag="VLE22_17305"
     CDS             49313..49783
                     /locus_tag="VLE22_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSB16215.1"
                     /translation="MHYPIREKLEEYLRGEAGPGELREFHSHMETCRECRDSVTGMRD
                     QARLLQCLRASDAVEPLPGFYARVMGRIESQRANSIWSFLLEPVFSRSLVVASVLLVL
                     LLGGYVASGEFDRPLNPSSPEVILAAEPNPSEMVGLNPQRDRNTVLLTLATYRD"
     gene            49790..50185
                     /locus_tag="VLE22_17310"
     CDS             49790..50185
                     /locus_tag="VLE22_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16216.1"
                     /translation="MSWENCSSWRNPRVLLSLTLIFLCGVVGGAMVMRFAVHGIVDKA
                     ETGLPGGEKAIALERMTKELDLSADQRRELETVLDDFVMYVQTLQAQMDEVRANGKQR
                     ILRILNDQQKQKFEKILVDVQARQTARVP"
     gene            50248..51678
                     /gene="amrB"
                     /locus_tag="VLE22_17315"
     CDS             50248..51678
                     /gene="amrB"
                     /locus_tag="VLE22_17315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HSB16217.1"
                     /translation="MSVAATHDSPYSGSWYPADRIELDELIDRLWKSSGQRTGAYLLE
                     NPLGFVVPHAGLVYSGTVAASAYRHIERCRPKRIVLLGFAHRGCPPGLWIPDVTSVRT
                     PLGEVRIDAEAVADLTVGGEFQILPEARLCDHSIEIQLPLIQRAAPDSSVVPVYVGQL
                     SDQARREEASALARRIRAGDLLIASSDLTHYGSAFRYQPFPVDNRVQERLRVLDFGVL
                     DAAASLRPELFLKTLLESSATVCGYEPVSLLLSAMRASEEQGEVFQQVIDYQTSGEIT
                     GDFHHSVSYGAAGYFPYSSFEVNSEAREVLLDLARRTLRQYQTTGRRPVSVAPGQDHA
                     VLEKRSAAFVTLHRGGQLRGCIGRNTSDAPLREVIPELTMAAALDDTRFGRVTPDETG
                     IDIEISLLTPMKRIVDRSDFRVNAHGVLLQSGYRHGLLLPQVATERNWGAEQFFLALA
                     RKAGFDPGVYDDPATKLHVFRAQIIH"
     gene            51706..54423
                     /locus_tag="VLE22_17320"
     CDS             51706..54423
                     /locus_tag="VLE22_17320"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSB16218.1"
                     /translation="MLLLTGPTGSGKTRHVLERVREALASRRTDFRLLVPTATMAEHI
                     RNELAREGFVPRRSLIGTLSGFVEQLAGDTPQVTGGQLNLIVEEVLASISPNEFREVA
                     SLPGFRTALAGLIDELTSAGCDSVQVRAAVVRRLPGRTLAAAFGDVFEEAERSWRRRG
                     AAARGERLRFAASRIREQGLPGVRAVYMDGFFTLSQPEIELVEALRAHADLTVTLPRW
                     EGSQQARARLLGMGFVEEEHTRARQQPRIVLLAARTTEEELGEIARRILAEASAGRPF
                     REMGVVVRSREPYVPALRAVLERFGIPARFYFAEPPVAYSPVRYLAGAVDAMLGGWDH
                     EETLGVLRMTSSAAGRCSDMDRFDFEVRKNLPGRGLAPLRLLARHPAIRTLIDSLAPL
                     DAWREGECQPGEWATRVKNLSSRLFRPVAPSDSPSRSLITVWRGQAAALRAWEGAANE
                     AAAALDGKGPVAFEPFWHAVNEALSETNVRVPDQRRNVVHVLDVYEARQWELPVVFVC
                     GLLERQFPLYHPQDPVLPDNVRSVLRQEGVTLRTAAERDREERFLFDLAVSRATSTLV
                     LSYPQFNESGDANLRSFFLDEIQATAVQEQSRPVRLAGGPTSRVDRPVVIRDPGLLDR
                     LVEKHASTSSSAVEGFLQCPFLFFARNTLGLEGPPRHPSERFDQLAQGDVIHCVLALC
                     IGSGVPLEEAFDAELRWVYKERGIPDGYRREAIRLELFRDLRRFLTDLELPPASKRLY
                     EHSFQFTLREGVRLRGRMDRVDVYEDGLALVVDYKYSHAQKVRDRRKGTEQGRFVQAG
                     LYLLAVKQVLGHEPAGMLFAGLRRETSLEGWHLPLPGLEAGADCTSEVLAELSATASG
                     QVAGVLDAVHGGRIAPSPADERLCEYCEFVDICRVESATGATAAGGFEE"
     gene            54420..57725
                     /locus_tag="VLE22_17325"
     CDS             54420..57725
                     /locus_tag="VLE22_17325"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSB16219.1"
                     /translation="MTLSPKQKAAVERSGQDVCVVAGPGSGKTRVLVERFCWLVSARG
                     VSPLRVLAITFTEKAATEIKQRLVNAFSGDAKVREQIERTYVSTIHGFCARLLRENSV
                     AAGLDPRFIVMDAPASAAELDHAADAALERLYTERPSEFRGLLDASYFSTDLASCLIH
                     VYHAQRVAGVPLARGRDASPPAESLSGIVQELYDLLSRVPPPKTTPQIGRITSIKGWL
                     ERAGTLERKPLGAEHFRLLKDFKCDLNTFKPGPDREAARDLRKRRIPRLQALLATEYY
                     ERFRALLVEAIERIDEAYRSRKRELSALDFSDLEEHTIRLLEGNPEVRDSLRKSFDYI
                     LMDELQDTNPLQWKIVNLIRSPERFFAVGDINQSIYGFRQAAPEAFREYRKGIESARW
                     KVDDLDENYRSRQAILSAAECVLAGAPGIELRELKAKRQFCRKTEPSVEVIVACAAKP
                     ADPADVEARWIARRIRELEGKLKIGSLTDPRAARFRDFAILVRANTALPPIERAFKEF
                     DVPYLTTGGQTFYEAREVRDLVLLLRTIDNPRDEIALAGVLRSPLGGVADETLLRMKS
                     HDRLGTVLRRLDHLDLSSFDAGDLERLRGFRDLLNRLRSVRDDVPPDRLLLDAIDGSG
                     YGSQLDGRARANVDKLLALVCDWHCAQPRPVNELVEDLEWIREAATEADAPPDDSSNA
                     VRVMSIHQAKGLEFPVVLLAALHRHTNDTQPSLCLAPEGRLGVRWRNPAGSGYVDDVF
                     HVESMALTDAREREEEDRLLYVAMTRAEEHLVLSFARTDRPGARAERVAGAFGIDLQQ
                     TDAGPRVFAPVGAHFEVRLFRAAGPPEAVEMREGDFSLPVEELLVPHGAASSQHDSSA
                     SVTAILAFEQCPRRYFLDRYLGWSGVSQPAAAEEYESQSAREELDEPDAAEFGLQVHA
                     LLAGAAIESPDAEAVELKARFDASALGRRVRRADRIEREFDFLLAVDDIVLHGQIDLW
                     FEENGRLVLVDYKTNDIRDEDAHSRADDYSLQLRLYALALERVCGRLPDEAYICFLRP
                     GRSVSVDLSEAALASARGSVGRFREAQNKLSFPLRESPQCRACPFYRGLCPAGRGQAA
                     KDADPPSSFQERS"
     gene            complement(57682..57903)
                     /locus_tag="VLE22_17330"
     CDS             complement(57682..57903)
                     /locus_tag="VLE22_17330"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSB16220.1"
                     /translation="MDELSEYLDGTIQEEMRQQLEAHVTECPNCWVMVDTTKRTIQIY
                     KGMDPQPLAEDLHRRLMNALEKKMAGPHP"
     gene            complement(57911..58537)
                     /locus_tag="VLE22_17335"
     CDS             complement(57911..58537)
                     /locus_tag="VLE22_17335"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB16221.1"
                     /translation="MGQPSTQAITFDESNLVERAQGGDARAFTELVNQYERKIFRLAK
                     HITQSQEDAEDVLQETFLKAYGHLPEFQRQSKFYTWLVRIAVNESLMKLRKRRTARTV
                     SLDEGIDTGEETVAREIAVWEDNPEERYSREELRRILDEAIDALPPIFRTVFVLRDID
                     ELSTEETAGVLNISIPAVKSRLLRARLQLRDRLTRFFKRKGDDVFAYL"
     gene            58647..60587
                     /gene="thrS"
                     /locus_tag="VLE22_17340"
     CDS             58647..60587
                     /gene="thrS"
                     /locus_tag="VLE22_17340"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HSB16222.1"
                     /translation="MSTISVTLPDGSRQEHPAGVRPIDIARKIGSRLADEAVVARVNG
                     SLYDLTRPLESDVTLEILTTKNPEALEVYRHSTAHLMAAAVLELYPSTKLGIGPPIDN
                     GFYYDFLRNEPFTPEDLEKIEAKMWELQGKDLPYERKLTPKAEGLKKYAEMGEQMKCE
                     LIEERADDMFSEYTLGPHFIDFCRGPHLPSTSRIKAFKLLSVAGAYWRGDEHSHQMQR
                     IYGTAFFTRKELEAYLRQLEEAKKRDHRKLGRELDLFSIQELAGPGLAFFHPKGGVVR
                     KLIEDWMRDQYVARGYSLVYTPHVARADLWKTSGHYNFYAENMFARMELDDAEYQLRP
                     MNCPFHILIYQDRMRSYRDLPVRLGELGTVYRYERSGVMHGLLRVRGFTQDDAHIFCA
                     PEQIEDEIVNCLQFAVDVLSTFGFDSYEAELSTWDGGKSGKYDGSPDQWQLAESALHK
                     ATDRLSMKVKVIPDEAAFYGPKIDVKLVDAIGRLWQLSTVQFDFTLPRRFGLEYIAED
                     GKAHQPLMVHRALYGSVERFFGILLEHYAGAFPVWLAPVQAAVLPITDRHVDYAKQVA
                     ERLKAAGVRAHLDDRNEKVNLKIREAQLQKVPYMLVVGDREAQNGTVSVRNRKHGDQG
                     AKPVEEFLAAIRSLIETKSVSE"
     gene            complement(60550..61098)
                     /locus_tag="VLE22_17345"
     CDS             complement(60550..61098)
                     /locus_tag="VLE22_17345"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB16223.1"
                     /translation="MIIVVSGPPCSGKSTLGDLLASRRGIVHLEMDAVRVRLLPDSAH
                     TRQDRAVAYRAMHLGAELLATSGATVIVNACYGRAEDRSDLEEAAGRAGAALYLIECT
                     VPPEIAVERAQQRWGNHPGIDLTPERVIELVSGYPFFSEGVTVDSTLPIDTALAKVEK
                     YLTTGSPLGTATRKRTWSRSVI"
     gene            complement(61095..63287)
                     /locus_tag="VLE22_17350"
     CDS             complement(61095..63287)
                     /locus_tag="VLE22_17350"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HSB16224.1"
                     /translation="MAIAVALAAALIGVYWFVWRPLPVTSGSLTAPIEAQATVTRDAL
                     GVPHISAASEQDALFLQGFVTAQDRLWQMDAIRRLAAGELAEIVGPGALNSDREARQM
                     RVRRVAEEHYKAITQNDRTALAAYAGGVNFFIETHRRQLPIEFTLLRYEPRPWSVVDT
                     ILSALQMESMLSSMWKIELRKENLLAGGDRQKVEALFPVRSGSEFQPGSNAWAIAGSR
                     TATGKALLAGDPHLEYSVPGTWYMVHLKAPGLNVSGVSLPGLPGVIIGHNERIAWSVT
                     NLQFDVQDLYVEKFDPAGGSYVYRGQVETARPEREVIRVRDGRPEQLVTWVTRNGPIF
                     LSQGGRYLSVRWAPAESGGFQYPILDLDRACNWGEFRAALARLPAPGSNFVYADLDGN
                     IGYQAAGRFPIRANYSGDVPVDGSFGEFEWRGFIPFEQLPSVFNPASGIIISANQNPF
                     PEDYPYRVGGNFSSPYRANQIRARLSSRKGWSTEDTLRLQTDEYSAFHHYLAREIAAA
                     CGRRGAKGAPVDEAAKLLRDFDGQMRNGSAAALIAELASQHVCRALAERASPGKGVVY
                     SYEMSRAVVERLLRTRPPEWAGDWDKLLVSNLANAIAEGQRTQGRDVTKWDYGRYNQL
                     GINHPIAGQLPLAGKYFNFGPVPFGGAPTTVKQVRFVPRVGPSMRMVVDFSDLDRSVL
                     NIPFGQSGQVLSRHYRDQWKSYLEGRSFRMQFRNIEAKETLRFTPTEE"
     gene            63368..64711
                     /locus_tag="VLE22_17355"
     CDS             63368..64711
                     /locus_tag="VLE22_17355"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSB16225.1"
                     /translation="MEEVYYGYRFLVLALIIGANAFFAGSEVALLSVRPSRLRELAEE
                     GSVGAQAALRVLANPERLLSVVQVGVTLASLGLGWAGEDTLFRFISGVFHPVLTPATE
                     KILHAVSFFLAFLVMAFFHVVVGEVVPKNLAIEKADRLAILVAPALLLFYRVSEPFVY
                     VIERSAAVLSRMLGLRGEPRGGGHSTEELKLIISASRGEGNLPKFEENVIHRILDLPD
                     LVVREIMVPRNDIVSAPVDSSLDEILRKMIEHQYSRLPVYEDRPEQIVGYLHLKDVAR
                     AREELRTSGLPRMPSPRLRLRSLLYKPLVVPETKPASQLVDEFREGHVHIAMVVDEFG
                     TIVGMVTFEDVIEQVFGKIEDEHDLRTQPPALEALAADVEGTITIRDLEMQYGIELPA
                     GAGFETLAGFLLYHLGYIPRPGDVVEYGGRRFTVLEMERNRIAQVRIEKVEGDTA"
     gene            complement(64718..65701)
                     /locus_tag="VLE22_17360"
     CDS             complement(64718..65701)
                     /locus_tag="VLE22_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16226.1"
                     /translation="MRYAAVAALMAACLLLLGVSPGPGLFFQRGVNFTSEWPDVYASD
                     RSAAILSKLPAYGINAVAIIPYGFARRDTPVIRFGGNRIWEKDGSVVRVARLAHERGI
                     KVLLKPQLWVRRGYPGDLDFSSPADLEPWFTQYDAFICHYASLASRIRADVFCVGVEL
                     SRVTRHEKHWRAVIARVRTIYAGPLVYAANWGEEFENLPFWDALDYIGLNQYYPLPDD
                     LSTGAVVGKIESVHRKYHRPVIFTEAGFASLEGSNRQPWDETPRKLSLEEQARCYDAV
                     LKAFYRKPWFHGVYWWKVGSNGFGGPEDGSHTPWGKPAMDVLARWYLRGGR"
     gene            complement(65707..66903)
                     /locus_tag="VLE22_17365"
     CDS             complement(65707..66903)
                     /locus_tag="VLE22_17365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HSB16227.1"
                     /translation="MGNGAPFPILGEGLGTTPRITSLLIKPASAVCNLDCAYCFYLDR
                     EADPYKELPARRMTADTLERLVDTYLFYSYPNSVFAFQGGEPTLAGLPFFEKLVDLQQ
                     QHGRGGQNVSNAIQTNAILIDERWCQLFRTYNWLVGVSIDGPEEVHDLYRFNKQGRGT
                     WKKVMQGIELLQKEKVDFNVLCVLSQANVEKPKEIYRFFRSLGIDFVQYIPLAEFDGN
                     GNRLPFTITPEQYGRFLCETFDLWWPERRKARIRFFDNLAEALAGQKPGTCTMHETCD
                     SYVVVEYNGDIYPCDFFVEKDWKLGNILIDSWPEIARRQRRYSFASKKTIAHPECQVC
                     EYQSICHAGCPKFRHGPRHNFEDLDYFCQAYKMIFGKSVSPLKKEVERLLRQAGAQEL
                     QAISPY"
     gene            66999..67730
                     /locus_tag="VLE22_17370"
     CDS             66999..67730
                     /locus_tag="VLE22_17370"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HSB16228.1"
                     /translation="MTPRELVFSVRSELAASADPKFREGLMWFFKEPVDPYGVRTPQV
                     RRIAASAYREVKKWPAAQRNAFCRELWASGKMEEGGVAICVYQRFRKQCAAREFHLFE
                     KWIDRYVGNWAHCDGVATWLIAALIGNEPGLIRLLFPWVASKNRWKRRAAVVSFLQEA
                     KQGRNTEAILKVAGAVLEDPDDMVQKGVGWVLKEAYPKRPREVMDLLRARKERAPRLV
                     LRIAAEKMTAAHKAEILSRASVARR"
     gene            complement(67702..68784)
                     /locus_tag="VLE22_17375"
     CDS             complement(67702..68784)
                     /locus_tag="VLE22_17375"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB16229.1"
                     /translation="MKGNLTRRNLFHLVAGAGLGSALRAQQAPPLEGGYVPEAVVPFN
                     RRSRVSVIRGEERRKIVCDSLVAIDDQIRPVLKQKKFVVIKPNNVSTVNQLAATHADT
                     LHGILDYLGPRFKGPVVIAESSAGDTLEGYENFGYNRLATERKSQKVSLVDLNREGKY
                     EVIPLLSPDLHVQPVRLAARLLDPDAFVLCAAILKTHNTVVATLSIKNMTLGAPLRSA
                     PGEPKWNDKRKYHGGIRQTHYNMMLTAQKMKPFWGATLIDGFEGMEGNGPASGTPVAS
                     RVAIASADYIAADRVGLEAMGIDAKWPAYLQYCWQVGLGQYDLSKIDVVGADLATVKK
                     QYRMHRDIERELEWLGPLNDVPPKLG"
     gene            68851..70020
                     /locus_tag="VLE22_17380"
     CDS             68851..70020
                     /locus_tag="VLE22_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB16230.1"
                     /translation="MKTNSSRRNFLAAGLGLPVAGIGAVSSSGPAIRPSASLPAPGGE
                     IRYRTLGKTGMKVSSVGFGCMITSDPSVIEKAADIGINLFDTARGYQGGNNERMVGAA
                     LKTRRKNIFISTKTGASNKEGALAHLETSLRELGTDYVDIWYLHGRSSASQLTEDLIA
                     AQQEAKEAGKIRFAGVSIHSGHGEVIPAMIRSGKFDVALTTYNFTMEKSIEGLIDSAA
                     KAGLGVVAMKVMAGGNRRVKPGDKMYDTLQRDGAMVAMLKWALRNQNVHTTIPSMTDM
                     DQLDDNLRAMTNAFTASDEKLLSARLEQIRPYYCRMCGECDGTCPKGLPVAEILRYVT
                     YADGYGQFALGREHFLSLPEEMTQVRCNLCPTCSVECKFGVNVARQLSRAQELFA"
     gene            70017..>70271
                     /locus_tag="VLE22_17385"
     CDS             70017..>70271
                     /locus_tag="VLE22_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HSB16231.1"
                     /translation="MRRRIFLGVLAAAATLSAQKPDCSVVPGWEQAGPARMYTADNLF
                     DYMNGNAEGYVIYSFHKMDGVTCKSGENTFVFDVSEMGSPE"
BASE COUNT        13240 a        21835 c        21687 g        13509 t
ORIGIN      
        1 gcacctcgcg atcacccacc gctgcaacct gcggtgccgc ttctgtcaca tccgtgattc
       61 cgtgtttgaa gaattggaca cggacggcat gaagcgggtc atcgaccgcc tcgacgagat
      121 gggcgtcggc gtggttagca tctccggcgg cggtgagcct ttgctccgac ccgatttcgc
      181 cgagattgtc gactatgcgg ccggcaaggg aatgtacacg aagctgacat ccaacggcac
      241 tctgccactc agccggtatg agcggttgtt gcgcagccgc gtcagggaga tcggcgtctc
      301 gctcgacggc gtccagggca acgaccttcc tcatagccac gtcggcccgc gcatcctcga
      361 ttcgattcgc tacctgcacg atcacctgcc gccgcagaaa cagttgacga tcaatgtcac
      421 ggtttcgggg accaaccggc accaggtcga ggagatcgtc gccttctgtg cgcgcgagtt
      481 ccccagggcg aaagtctggc tgaatccggt aatggccggc cagggagcgc tccgcaccgc
      541 cgaggccgtc agcaccccac cggactacct gcgccgctgc cggtcgccga atctgctttc
      601 ggcgaagttc tacatcgaag gggtggagaa acagttccgc agggggcatt tcgattgggg
      661 gtgccgggcg ggcgccacgt ttttcgatat caagccgaac ggcgatttct ggctctgcca
      721 ggaccagccg tcgcgcactc cactcaacat cctcgatccc gcgttccgcc ggaaactccg
      781 tgctagcgac ttcaggtacc gaaaggaatg caccgggtgc acctattcct gctactacgt
      841 gacgcagaac ggcctcagcc cccgccgctg gcctgacatg gccctgcttt ggtggacgtc
      901 caacaccatg cacggtgatc cctgccggca ggccgccgga cgctacggct ggctcgccgg
      961 actcgggatg taccttctcc tgaaattgcg aaccggcgtc tcctccatgg ctgccgcggc
     1021 tctgccagtt ttgcccctga tgggagcgtg atgtcctctc gtgcggcacg cggaaagcaa
     1081 agcagtcaac tgaactggct ggcgcgcccg gagggactcg aacccccggc ctactggttc
     1141 gaagccagtc gctctatcca gctgagctac gggcgcgcgg ctggaaccat tatactggtc
     1201 cgcccgctgc cagcattgtg ccggaatccg ccagcttttc aacccggtga accgggagcc
     1261 cggcatccgc tataattcac gaggcggtaa tactgtcctc gaaaggaact cccatgcgga
     1321 agaaagtcac tgtggtcggc gccggtaacg tcggcgccaa ttgtgccctg cgcatcgcgg
     1381 acaaggaact ggctgacgtg gtccttgtcg acgtcgtcga gggagtgccg caaggcaagg
     1441 ggctcgacct gttgcagtcg gggccggtac aggggtacga cgtcactgtc accggcgcca
     1501 acaactacga aacgacggcc gaatccgaca tcgtcgtcat taccgcggga ttcccacgca
     1561 aacccgggat gagccgcgac gatcttctca tcgccaacta cgaaatcgtg aagaccgcca
     1621 ccgaacaggc ggcgaagtac tccccgaaca gcatcatcat cattgtgacc aatccgctcg
     1681 acgctatgtg ctggactgcc tacacggtgt cgaagttcag caagaaccgc gtcgtcggca
     1741 tggccgggat cctcgatacg gcgcgctttc gcacgttcat cgcccaggaa ctgtcggtct
     1801 cggtggaaaa catcaccgcg ctcgtcctcg gtggccatgg ggacacgatg gttccgctgg
     1861 tgcggctcag cagcgtctcc ggcattccgc tgacggaact gctcgaccag tcgacgctcg
     1921 accggctcgt ccagcgcacg cgggacggcg gagccgaaat cgtccggtat ctcaaaacag
     1981 gcagcgccta ctacgcgccc tcagccgcgg ctgtcgaaat ggtggagtca attctgaagg
     2041 acaagaagga ggtcctgccc tgcgccgcgt acctcgaagg cgagtacggc atcaatgggc
     2101 tgttcgtcgg cgtgcctgtg aagttgggcg ccggcggcat cgagaagatt tacgaggtca
     2161 agctgtcggc cgaagaaaag gccatgttga acaaaagcgc ggccgcggta caggagctga
     2221 tcgagatcct gaagccgaag ctcagttgag cggaaggcag gccgcgggct tccaagggca
     2281 gcgcgcggcg tgatcccgct cgatctcgag cagacggttg tacttggcaa gccgttcgga
     2341 gcgggccacg gatcccactt tgatctgtcc cgctccggag gcgaccgcga ggtccgccag
     2401 gaaggaatcc tccgtttccc cggagcgggc tgaaatcaca gcccgaaagc cggcctcgcg
     2461 ggccatgtcg atcacctgga acgtttccgt cagcgtgccg atctgattca tcttgaccaa
     2521 cacggcgttg gcggcgcgct cccggatgcc ccgctcgacg cgggcggggt tggtcgtgaa
     2581 aaagtcgtcc ccgaccagtt gtatcctcga gccgagcgct gcattcagcg atttccagcc
     2641 atcccagtca tcctcggcca gcccatcttc gatggagatc accggatagc ggcgactcca
     2701 gccgtcgagc agctcgatca tctcggcgcc cgtcagcttc ctcccttcgc tgcgcagttc
     2761 gtaagcgccg tctcgcagga agtgggtggc ggcgacgtca atagcaatca gaacctgatc
     2821 tcctgcgcgg aattcggcca actcgatggc gcggaggagc accagaagcg ccatctcgtt
     2881 gctcgcgaga agcggccccc aaccgccctc gtccgcgaga ccgctgacag gccgcccggc
     2941 ggcctccagc acctcctgcg tggcacggtg gacgcgcacc gccgcctcga gcgccgccga
     3001 ataggactgg aagccgtgcg ggatgatcat gaaatcctga aactcaaagt tgtgtccggc
     3061 gtggatgcca ccggaaatga tgttgatcat cgggaccggt aagcgcgggg tccggcctcc
     3121 gcaaaggtat tcccatagtt cgaggccacg cgcgctggcg gctgcgcggg ccactgcaca
     3181 ggagactccg aggattgcgt tcgctccgag acgcgacttc gcctcggtgc cgtcgaggga
     3241 gatcatcagg cggtcaagac ccgcctggtc ctcggcgtcg gagccagcca gtgcgggagc
     3301 gagaactttt tcgaccgaag caaccgctcg ataaacgcct ttgcctccgt agcgcgccgg
     3361 gtccccatcc cggagttcca ctgcttcgtg cctgccagtg gaagcccctg aggggacctg
     3421 cgccgccccg cgtgtgccgt cggaaagctc tacgtcgacc gagagcgtgg ggttgccgcg
     3481 gctgtcgaga atttcgaggg cgcgcacgcg ccggaccgtc aatttcaacg ttctaacctc
     3541 cggaagattt cctgcacagt gggtgggggg gtgagaatca gccgtctcgc aaaactccgg
     3601 atttggtcgc gagctccgga atcacgaatg tactcggcgg gagacttccc gtcgatccgg
     3661 gcgagcaaca gaccaccgag gtggcgaatc gttcgttctt caaaaccggc atcctcggcc
     3721 gggtacccgt cgagcagagt ccgccagaaa acatccgcca gggcggcgaa ttcggccgcg
     3781 acgtcgggca agtggaatga cttgagcagc aggtgattca acagaaatgc ggcgtcgaag
     3841 gcgggatcgc cgtagtggat cacttcgaaa tcgatcgcca tcgcggacga cccgtcaacg
     3901 aggaagttct tcgggctcca gtcgccgtgt actaacgtca gccggaagcc gcggcagtcc
     3961 tcgaccagac gatcgaagtg cggcctcaga tcggggtgac gggaggcggt actgcggtaa
     4021 tacgggtcga gacgcaattg gtcgaacacc gtctggtcgc cgaagatact ctcgaattcc
     4081 ggattgcgcc aaccgagccg aatcatgccg gcgagcatgc gggcaacccg agcggcgatt
     4141 tcggcctgaa gaagccggcc aagcagcagg gacttccagt tttgcgctga cgggggagcg
     4201 gcgctcattg cgaaaaggca gttctcgcga tcctcgaaga gcacgtcggg gaccgcgcct
     4261 ggttcgaggt ggggagcaag ccgccgcaac gcctcgcact cgcggaagat tcgatgacgg
     4321 tcggaaaacc agtcctgctc gacgcgaagc ttgccgaggg cttgcttgag cacgaacggg
     4381 gcggcagggg tatcaaccaa caagaccgtg ttggagattc cgccgccgag ggtagttatt
     4441 ttcaccaatt ccggatcgag cccccgggca gactggaggt agcgggctgc gttcagtgcg
     4501 tccagttcga acaatccaca catgatacca gcggcaagcc ggacggatcc ggcagccgcg
     4561 gtcaggctcg ggagaacagc gaaagaaggc cgccggcctg tggcttgcga gcggcggtct
     4621 cccggtcggc gaggcgggcg gcgagttcgg caaggctctt gccgaactgg ctgtttccgg
     4681 taaccggtct cgcctcgatc agggcgctat cgattgcttc ccgatcgtag ggcagcagtt
     4741 gagtgatttc tacacccagg gcggattcga cgtctccggg ctcaagaccg tagtccttgc
     4801 gaaaacgatt gaccagcaac cggacgcggt ggcgggcgat gccgttcctt tccaggcagg
     4861 cgagcgtccg ttgcgcgccg tagactgccg ggacatcggt ggtcacaacc agcagaacct
     4921 catcggcaac tctcgccgct ttcaggttcc acgcgccgta agggtttcca agatcaagga
     4981 agacaaggtc atagacgcct cggcagtact cgatgagctc ggctagttgg ggcgcgtccc
     5041 cggcgcagtc cgagggattc tcgggcgagg gcaggacatc gagcccacgg tagccagaca
     5101 caagcgcctt ccacaggtcg gcgtcgagtt cgttcacatg gcttagcgca tcggcgaagc
     5161 tgtactgcgg cttcagcttc cagagaaaag agagggtgct tgtgagcgga tccagatcca
     5221 cgagcagtac cttcgagaca ccgagctgct tggcctgaca cgccaggttg gatgcaatag
     5281 ttgtggcgcc gcacgaccct ttcgccggta cgacgctaac caccttcccc gcgccgttcg
     5341 ctacgccgcg accctgcggg aagagagccg cgaccttctc gagggctgcc ttcagttgct
     5401 ccttcgagaa cggccgaagc agaaagcctg cggctccccg acgcaaatgc cggaggatcc
     5461 gttccggctc gttgcggccg agcaaggcga tgacgggcag cttggtgggc tggtcggaga
     5521 gctgggcgag gatacgatca gcctggtccg gcgtcgaaca gacgtccagg aaacaaagat
     5581 ccggcggccc cgaaacgagc agttcggcga gcggtcggga tgccaggtag gttttgcagt
     5641 cgaggatctc ccctacgata gtctcccgcg aaaggacggc accgagttcg tcggccatac
     5701 tgcggtccgg gcacaccagt aacgcccgct tgaaattgcc gcctgccaat ggttctcgta
     5761 ccctcaaact acatcggcag gtggtcaaga aaaggtgaga gatggcgcgg gagccggcaa
     5821 ctaaagttct tgaaaagcat gaccgatctc gtctgtgtgc gctcgcctgt accaaccctt
     5881 ccatgactcc cttatcctcc cgaatcaccg tcgtagtctg cgacacgcag cctgtcacgg
     5941 ccgagggcct acgggctgtt ctgagcgctt gcccggatct ggagttcatg cgctctgcac
     6001 actcgctggc gtccggtgcg gaggtgatcc gaacagagcg cccgaaagtg gcgcttctcg
     6061 acaaggcgtt cggcatccac gggctggtgc agtggatggc ggacctgggt ccaatgctga
     6121 gggagacttc ggtagctgtc tggggggtgt cgatcaacga aacggaagcg ttacggttta
     6181 tccaagccgg agcgaaggga gttatccgga agacggccga cacttgtgtg ttgctcaact
     6241 gcttgcggac aatcgccgcg ggatccacct ggatggagga ctcgattttc cgtgatttcc
     6301 cgcgcctggt gcgttgtccc cactcggatc tcaccccacg cgaactccag gtgctgtcgc
     6361 tggtggagca cggcatgaaa aaccaggaga ttgcgcgcga actggggatc cgtccaggta
     6421 cggtaaaaat ccacctgaaa cacattttcg agaagactgg cgtccgaggg cgacacggac
     6481 tggcgctttc cggtcttcgc gagagaggcg cactctcact tccatcatga acaaccgctg
     6541 cgaatcgaca ccgtatttcg gcgcatgttc taactaattt cggtattctg ggtgcgtgca
     6601 gcgagttctg cggaccgtga tccttgcgct ctgggcggcc acgattttcg gcaactccgc
     6661 gtcgttgctt gacattcttt cgggcgaact ggaacggaat tttcgtgttc tacatgaaaa
     6721 gggagacccg ccggcatatt acatcgcgta tgccgtgacc gatcaggaat cgcacaatgt
     6781 cggcgccacc ctgggggcgc tccaggtggc cagccatgcg tcctcgcggt ggctggacgt
     6841 caccgtgcgg gtcggttcgc ctgagttgga caactaccac cggctttccg gcgatcggtt
     6901 cgccttctca tcggcagtgc ggttgccgct ggagaatgtg ccggccgcca tatgccagcg
     6961 actttggatg gataccgatg gcacgtaccg ccgggcggcg gaacgcctga tcaaggtccg
     7021 gactgcggcg caggtgaagg cagttgaggc cgacagtgcg gccgattttt cgaaagagga
     7081 gccggcaatc tacgaggacg cgccgcagcc cctggaattc tcgccgcagg actggcaaga
     7141 tcgggttcga aagtggtctg ccgcgtttgg ccgttatccg cggatcctgg catccagtgt
     7201 ctcagtctcc gcgcaactgg agacgaagta tctcgtcacc tccgaaggct cacggatcga
     7261 gcagggcaag gggctggctc gaatcgcgct ctcggcgcaa accaaggcgc ccgacggaat
     7321 ggatctcacc agcgaggaaa gtttcgagtc ggaaacggtg agggggctgc ccggagacaa
     7381 ggaagtcctg ggggcggtcg aacgtgtcgc gaaggagttg acggcattgc tgtcggctcc
     7441 ggacgcggag ccgtttgtgg gtccggccat cctgtccgga cgcgcggccg gcgtcttctt
     7501 ccatgagatt ttcggccacc ggatcgaagg gcaccgccag aacgacgaaa ccgaagggca
     7561 gacattcgcc aaggctgttg gcacgaaggt acttccagat ttcctctcgg tgttctccga
     7621 tcctacgatc aggaagctgc acggagtcga cctatccggt cactacctgt atgacgatga
     7681 aggagtgaaa gcacgcaggg tgacggtagt ggagagcgga attctcagga atttcctgct
     7741 ttcccggtcg ccgatgccgg gtttcgacca ctcaaacggc cacggccggc gccagcctgg
     7801 gttcgatgtc gtttcgcgcc agtccacgct gatcgtggag tcgtcgaaga ccgtagacga
     7861 ggcgcggctg cggggattgc tgatcgagga gatccgccgg cagaagaagc cgtacgggtt
     7921 gtacttcgag aaggtgaccg gcggctacac gcagacgtcg cgccggggcc tccaagcgta
     7981 cacggtcgtc ccgatcatcg tttaccgggt ttacgccgac gggcggcccg acgagctcgt
     8041 ccgcggcgcc gacatcgtag ggacgccgct ggcgagcttc tcgaagatac tggccgcttc
     8101 ggatcagccg ggagtattca acggctactg tggcgccgag tccggcagcc tgccggtttc
     8161 ggcggtagcc ccgtcgctgc ttgtctcaga gatcgagatt cagaagaagg agaagggcca
     8221 ggacagacca ccgttgctgc cggcgccctc cggagaatgg aggtcgcatt gatgccgacg
     8281 gccgccgtgc ggcagagatc ttccgcgcca gccgctctcc tcgtgacgct ggttttaccg
     8341 ctcgcggctg gtcagggaac gccgcctcgt gaggacgtca ttctgcgcgc gatgcacgac
     8401 gaactggagc gggcgcgaga gctgactgtc gagacgctgg agtcgcccta ctacgtagag
     8461 tatgcgctcc acgacggcga aactttgatt atcgacgcct ctttgggcgc tctggcgggc
     8521 gtgcggcagg cccgttttcg gcaacccacc gtgcaagtgc gcgtcggtag cccgcaattc
     8581 gacaacacga accatgttct cagcgactac atgttcggtg cgcgctacga cgccggtcag
     8641 acaccgctgg acaacagtta cgatcttctg cggcgatact tctggctcgc cacggaccgg
     8701 gcttacaaat cggcactcga ggtcatcgcg cgcaagcgtg ccgcgctcag gaacttcaac
     8761 tcgggcgaac agccgccgga tttcgccaag gcggagccat cgaagattat cctccgcgtc
     8821 gaaacggagc agctcgacgt cgagtcgtgg aaggcccggc ttcggagtct ctccgctgtg
     8881 tttaccggct atccggcagt cgtgagctcg ggcgtccaac tacaggccaa ccagggcgcg
     8941 ttctactttg ctaactccga gggcgccgag gtccgcattc cggagacgct gatctttctg
     9001 gaggttcgcg cccgcgcact ggctcccgac ggtatgatgt tgcgggactc gatggtcttc
     9061 caatcccgta gcgtctcagg cctgcctacg gatgctgaaa tgcagcgggg cattgaggag
     9121 gtcggcgaca gcctcaaggc actcatcgaa gctgctccgg cgaacacgta tacgggcccg
     9181 gttctgtttg aagcccgcgc tgcggcccag atgtttgcgg aagtactcgg cagaagcctc
     9241 gggctcaggc ggaagcctgt ttccgagccg ggccggccgc tgccgtcgcc tcccaatgaa
     9301 ttggaaggcc gcataggatc ccagattctg ccttcgtgga tggatgtcgt cgacgatccg
     9361 gcccaagcgg aatggcgcgg caggccgctg ttcggacact acctggtgga tctggagggt
     9421 gtgacgccca agccgctgtc tctggttgag aagggtgtac tgaaaaccta cctgctcacg
     9481 cggcagccgg tcggtggatt ctctgaatca aacgggcggg caaggcttcc aggcggtttc
     9541 ggagtgaagg cgccgggatt cggcaacctc ttcgtccggg cgagccagac tgtcgcgcag
     9601 gcagagctga agcggcaaat gctcgaactt tgccggttgc gggacaaacc gtatgggatc
     9661 ctgattcgaa agatggattt cccctcgtcc gcttcgcttg aggagtttcg ccggctggcg
     9721 cctcggatgt cacaggtcgc gtccggcgcc cgaccggtaa gcttgccggt tctggtgtac
     9781 cgcgtctatc cggacggcag ggaggaactg gtacgtggat tgcgcttccg tgacctggcg
     9841 gttcgttcgc tgcgggacat tgtggccgct tccgatgagc accatgtttt tgaattcctg
     9901 gataataatg cgccgtttgc gttgatgggg gcaggcggat ttgccgccga gagcgccgtt
     9961 gtcgcccccg ccgttttgtt tgacgaactc caattggacc gggccgagga gcagattccg
    10021 aaaccgccgg ttgtgcctcc gccaccgctg tccggcacac gctgactatg ggcatactca
    10081 accggcgggt ctttcgcgag attgcttccg gggcgttact agggggcgtt ctcttcactt
    10141 tcgtcctctt tctacagcga atcggccggc tttttgagct gctggtccgc agttcggcat
    10201 ctccctcgac ggtggccgaa ctttttgccc tggtcctccc gcccacctta ccgtttaccg
    10261 tcccgattgg cgttctggtt gggatcctgt tgggcctgag ccggatgtcg agcgacgggg
    10321 aaatcacggc gatgcggtcg gcaggaattc ccagccggcg cctcctttcc cccgtgattc
    10381 tgttcgccgc cttcgccacc gcgatcacgg cggctgcttc cttatggctt accccctggt
    10441 cgatccggga gacctatcga atcgtgaacc ggctggcggc ggcgcagttg acggcggaga
    10501 tccagccgcg agtgttcgag gagcaattcc caaacaagat cctctacgtc gccgacgtca
    10561 tctcggggcc taaggtccgc tggcgcaacg tcttcatggc tgatctgact ccccccgcgg
    10621 agcgccgggc cggcgccgga gaaaccggcg agggcccgcg gattactgtc gctcgcgaag
    10681 ccatcgccgt tccggacgtg gcgcggaaca ccatccagct ttcgatgatc gacggcagta
    10741 cgcacgaggc ggcaaaggac cccgcggagt actacaacag cgccttcccg cgaggagatc
    10801 aggtccttga cgcatcgccg cctgacgaga ggcgagcccg tccgtttcag gaaacggata
    10861 cgccgccgct gttccggatc gcgaaggatt ccgtggacgc gcgcatcgaa ctgcatcagc
    10921 ggctggcact cccgccggcc tgcatcctcc tagctctggt ggggttacca ctcggcgtct
    10981 cggtccgcaa agccggaaag tcgtcggcgc tcgtgctgac ggtgttcctt gcattcctgt
    11041 attacatggg tctgatcagc ctgatcgggc tggcacgaga gaagacgctt ccggtcgagt
    11101 tggcagtctg gactcccaac gcgttgttcg cgttgggcgg actgctgctg gcatggcgcc
    11161 tcgagaagcc tggcgaccgg gacgtcgtcg gcgcaattcg tggccgactt ggctcagcgt
    11221 ggaggtggtt gcgggagcgg tttgtgcata ccaagaacgg tctccgcagc gacgccaccg
    11281 ccggccgcat gcccctgctt ccacaggttc tggatacgta cattctttcg agtttccttg
    11341 tttttctgac tctctcgctc accagcttcg tactgatgac ccacgtgttc acgttcttcg
    11401 agctcctcgg cgacatcatc aaaaaccaca tcccaatgac gcgcgtgctc acctacctgg
    11461 ttttcctgac gccgaagctc atctatgact cgacgcctgt cagcgtgatg gtcgctgtgc
    11521 tggtgacctt cggggtactt acgaagcaca acgaggtgat cgcctttcgg gcatgcggag
    11581 taagtattca tcggctggca atcccgatca tgctggcgag cacactctta agcgtcaccc
    11641 tgttcgcgtt cgactactat tacattccgc actcgaaccg gattcaggac gccatccgcg
    11701 ccgagatcaa agggcgcccg gtgcaaacgt acctgcgacc cgaccggaaa tggatcttcg
    11761 ggcagggacc gcggatctac tactatcggt actttgaccc tgtcgcgaac atgatggcgg
    11821 gtgtcagtgt gttctacctc gagcccggtt cgttccgcct ccggcgacat ataacggcgg
    11881 agcaggcgcg ctggtctccg accctgagaa cctgggtgtt cgagaacggc tggagccgcg
    11941 acattcgcgg gattcaggag tctaacttcc agcagttcca ggcgacgact tttccggagt
    12001 tgaacgagcc gcccggctgg tttctcaagg aggtcaagca ggacaagcag atgaacttcc
    12061 aggaactgtc agcttacatc ggggaacttc gccaaagcgg cttcgatacg gtccggttga
    12121 gtgtccagtt ccacaagaag ttctccgtgc ccctgtttgc gctgatcctc gccatgattt
    12181 ccttcccgtt cgcattcgtt gccgggaatc gtggagcgtt ggcgggcgtt ggcgtaagtt
    12241 tcggtattgc aatcgcttac tgggccgtca gccagctttt tgaacagatt gggaacgtca
    12301 atcagttgcc ggctacccta gctgcttggg caccggacgc cgtgttctcc cttgctggga
    12361 tgtacctgat cgcccggatg agaacctgag agcggcccga ggggccagag ctaatctccg
    12421 agtaccaggt actaggactg tttggtggtc cgctaggggc ctatttgcct tggtttcctt
    12481 gacaacagca gcgaaatgca gcagaatttc agagtacggt tgggattacc gcgcacggaa
    12541 gctgtcgaca gactatttag tgaaagcgag gagatgaaac caatgacacg ggcgcttatg
    12601 tttctgctgg ttgggttgct ggctgccggg tccgcagcgt tcgccgctcc aatctacttc
    12661 aatgactttc agagtagcgt tggcgcgggg tggtcaaata cgacgacttc cactacgccg
    12721 gccggctgca caacgtgcac gacctacctt ggacagttcg gcaaccagag cgtcactctc
    12781 acgctcaccg gtttgccgac aagcactcag cttaccatct cgttcgacga attcatcatc
    12841 cgctcgtggg acggaaacgg ggaacagggc tttggacctg atacagccgc gttctcgatt
    12901 gggggcgcca atactctgac cacgacgttc gcaaacggcc tggtagggaa tacgcagaac
    12961 tatcctaccg caggcagcgc cgcacagacg ggcgccgctc aggtcaacac cctcggctac
    13021 actttcatag gagtgccgcg agactcgatt tacgcgctgt ccttcaattg gttcaacacg
    13081 agttctaccc aaacgtttac ttggagtgca agcggtctcc aggaacttgc cgacgaatcg
    13141 tgggggatcg acaacgtttc cgttagcggg cccgacgagg cgattcccga gccgggtacg
    13201 gcgctcctgc tcggcagtgg ggtgctggcg ttccttctct atcgccggcg tcgcagcgcg
    13261 taggccccca gtcaggcgag aacgtttcca gatcccgggt tcttccgaaa ggcggcccgg
    13321 gatttgtatt tcttgcttgc ccgcccgtcg agcggcgctt gcatcgagtg atcggcaatt
    13381 gctaccatcg cagtttcctg ggcagtcctc agcgcaagga tttcgtctgc gcttcgctgc
    13441 ggcctgaccg ttcgctgata tgcgcaaagt tcccctcgca gaatccctga agattatcgg
    13501 ccgcggttcg ctgaactgga ttgcggagcg gccgatcgtc gtgtcgtttg aagtgaccga
    13561 cgcctgcacc tgctactgcc ggcattgcga tcacggggga cctagagacg gatcacgcaa
    13621 tcttaaggct tcggacttcc ggcgctacgt ggatgcactg cgcccgtgcg tggtacaggt
    13681 ttccggcggc gagccgctga tgagacggga catcgttgac attgtccgta gtatcaagtc
    13741 tgacggacgg atcccctaca cgattctggt ttccaactgg tcggagatga ccgaggagat
    13801 ctacctgaaa ctccacaaag cgggagtcga ccagttttct gtcagcctcg atttcccaga
    13861 caacagacac gatgatttcc gcggctatcc tggcctctat tcgcatctga gcgatctcgt
    13921 cccacggctc gcaaagtacg gttacgatga catcgtgctg aactcctgca tcacgagcgt
    13981 gaatgtccga gagatcaacg cgatggccga caaagcgcaa gagtggggcg tcaacatttg
    14041 ctatagcgca tactccgccc ggagaaccgg ctgccgggat tacttcctca atacgagcga
    14101 ggaactggaa accctgaacc aagaattgaa ccgggtggaa tcgcggcgag acgaaagcaa
    14161 ctggattgtt aacgccacca cgacgctgga ggcaacccgg cgctattttg agaatcgggg
    14221 cgctcctggc tgcaaagcag gtctgcggtt ccttgtggtg accgcggacg gccagcttca
    14281 accttgctcg atgcagttca agcgctatcc tctgcaggaa caggctcgaa tggtccagga
    14341 gttcaccgcc aacaacactt gcgacgaatg ctacgtctca atccgttcct acctcgacaa
    14401 gtcgtttcct caacttctgt gggaaaacgt cgccggcttc ctgtcgttca aggccggtac
    14461 acgcgctcgt agttccggga attgacgcag ggctagccga ggaacacgat gtgctcgtcg
    14521 ggataatcga gcgcaagcat catgagcatg tagtctttca ggtggcgggt cagcaggaaa
    14581 ttctgtttta cgtgctgatg gccgttttcc cccagccacc ggcagagttc cggatcgcgc
    14641 aatagctgga ccgtccgttg cgccgcccct tcgggcgagt ggatcagaaa cccggtgatg
    14701 ccgttgatga tctgctgttt gatgcctccg accgcgccac caatcaccgg cttctttttc
    14761 cagagcgcct cgcttacggt caggccaaag ccttcccgga tcgatttctg catcacgacg
    14821 gtggaagcgc ggacaagcgc gttgatctcc aggtcgctga acggaggcag caggagaaca
    14881 tggatgtccg gatcctcccc ggcacgctcc tgaacttcct ggagcacctg cgcaccctcc
    14941 gggtcatcgg gggcgcctcc accggccaga accaactggc aatcgacccg cttcctcgtc
    15001 atgcggtagg cggcaatcac gccaagggga tccttgaggc gatcaaaccg ggaaatctgc
    15061 gtcacgatcg gacgatccgg atcgagaccg aacttcgcga gcaccgtgtc gatgcagccc
    15121 ggctcgagat cgcggttctt gtcgctcagc gggtcgatcg atggcgcgac catgtactgc
    15181 gggatcggca actcctgtgc gaaatcgggc atcgagaaaa tcgccgcgtt gtattgctcg
    15241 acgtaaggtc gcaggaaagc ccaaacctcg ggattcggcg ccgagacgtc aatgtggcag
    15301 cgccacgtcc acttgcgacc gatttccggc ttgcgaagaa tgagtccggc gggttgcggg
    15361 tcgtgaatca gcacgacatc tccggtgaac tccatctcgc gcaggtttac ctccgtgttc
    15421 gcgcgaaaaa cctcgaacat tcggttcgtg acttcctgcg ggtctccgtg cagggcatta
    15481 tggaaagcct tcgtcacttc gaagaattcc tggtcgccct tgatcacgtc ccagcgagcg
    15541 tcgacaccga cttcgcgcaa tagcggtacc agcctcgtta gaatctcggc aacacctcca
    15601 cccaccggcg tcgagttgat gttttgaagg cagcggcccc gtacacggtc cgctacaacc
    15661 tggatctcct cgatgacttc cggaccaaca atctcctcgt agtctttcag cttgcgagcc
    15721 atgttacgct acttcgtgcc ggctgccgca gcggatcatc tcgtcgcgga gttcgtccag
    15781 cgtcatgccg tagggatcga gctggtcgat ctcccgggcc agtgcctcct gaccgcggta
    15841 gcacaaccac tgtgagaaat cgttcgtcgg ttcgccaagg cggagacgtg cctggaaaaa
    15901 gtggaagaac agagacacgt ttgagacacg ccgcaaggtc gagaaaaact cgggaacatc
    15961 gtccgccacc agacctgtgg gcatgacaaa gctctgcgac tcgcagaagt ggaattcctc
    16021 acctgggcgg cacgaacggg tgtcaccggc atccgcgcgc agctggcttt cgatgcgagc
    16081 gagcagttcc tcccgaatct gtcggacggt cgtacaggcg agcagatcga cggccgtcag
    16141 ttcctcggac agccgcctgt actggagcgc atctccgacc cagttcgcga actcgttatg
    16201 gtagacaggc ttctcaaaat gatgcgagag gaactggtga tgcgtatggt agaaaaccga
    16261 ggcacctgac acatcgctca ggtggtgcgc cagttcgtcg agggttctcg ccctgcggct
    16321 ggtgagccgc accaggcggc gttcggtgta aaacgtgaat ggggacattt cgttcactga
    16381 caggtctccc cgatccattt aagaaattgt ccgacgtcat ccggcccggc gacatagtac
    16441 gccgcggcgg tccctagcgg gtcgcccact ttcacggtca ccccgccggg aagcgccgca
    16501 aacgcatctt cgtcggtttg gtcatccccg gcgcagatcg gcaacaccgt ctccccgtct
    16561 gccagccgcg agcatagcca caaagccgcc gaacccttgt tccagtcgac cgcggggcgc
    16621 acctcacaga tcatctttcc catccggagg gcgaggccac gatcgcgccg gacgacctcg
    16681 tcccagacgg cctcaatcac tcgattttgc gactcaggag cgttccggta atggacagcg
    16741 gcggtaagtc ccttgtcctc cacttcaacg cccggcactc cccggagtcg ccttgaaaga
    16801 gacatacagg cccccgcgat caacggacgg agccgttcgg cttcgggctg gacgaatcgg
    16861 aaacccgggc cttcgatttc gagcccgtga ttgcccgcat aggtcaggcc cgggatgccg
    16921 acgcgtgttc gaacgtcatc caaagcccgg ccactgatta ccgccggcag agtgctccgg
    16981 gatcccacca gagatacgag caaatcccga actggcagcg gaaggcgggc agccgatgga
    17041 tccgcgacaa tcggcgccaa cgttccatcg aaatcgagca gcagtagaag gcgcccggaa
    17101 ttctggatgc gtgcacggat ttcgtcaata cgctcccaga gagggtgcgc cacgtcaggc
    17161 ttagtggacg gcggcttcaa agtggctaac atcggcgttc tccggaaaat cgaacttcag
    17221 aatggtggag aggatctttc ccgcccaccg gtagatgtta ttctcggaaa caacgcctcg
    17281 cagccgctgc atgcgcctcc gtcgctcctc agtcggcatc tcaagggcct ggcggacggc
    17341 ttccgccatc tcttcctgag agaacgggtt gacgagcaga gcgtcggtga gctcgtacgc
    17401 cgcacccgcg aaccggctca aaatcagcac gccgtcgtca tcgttgcggc ttgcgacgaa
    17461 ctccttcgcc accaggttca tcccgtcgtg gagtgaactc acaaaacaga accgcgcgag
    17521 tcggtgcaac gccatcatcc gcacggaacc cacgtgcctg gagagtagga tgacaggacg
    17581 ccacgagtcc gtgctccatt tccggttgat ctcgtcggcc agcgcttcga tctcgtagcc
    17641 cagggaccgg taacgcccga tgtcgacgcg gctgggaaca ccgacttgaa tgaacaccag
    17701 cttacctcgg tactccggat attcgtccag cagacgatcg attgcctgga gccggtcggg
    17761 aatccccttg gtgtaatcga tccgatcgag cccgatgcca acaagttcgt cgcccagtcc
    17821 cagttcctgg cgccagcgtt ccatctccga ctcaacctgt tccgaagaag cggaggcgga
    17881 atgcgactcg aaatcgacgc tgatcgggaa cggccgcacc gccgtagtcc gttctctgcg
    17941 agtaatttcg tacagttccg ggtcgattcg ggcttccagc gttcggtcta cagtgtcgag
    18001 gaagttctgg caatggtacc ggatgtggaa gcccaacagg tcattcccca acaacccgtc
    18061 gagcagttcc tcaccccaag ggaaagcccg gaagacctcg cggtttggcc atggtatatg
    18121 ccagaactgc gccacaaaca gattcggcaa gccggagtct ttgagcatcc gggggagaag
    18181 gccgaagtgg tagtcctgga tgaacacgaa ggtgggacga tctccgatct cgtccagcac
    18241 cgcgcgggca aagatttcgt tcacctcgcg gtaagcgcgc cagtcttccg gacggaacac
    18301 cggacgcgtg aatgcaatat gacagagcgg ccagagtccc tcgttggaga gaccgtaata
    18361 gtagcggttc tcctgctcct tcgtgagcca gacgcgcctc agcgtgtact tctgagaatt
    18421 tcccggaggt accgctacgt ggttgttcgc atctacggtc cttcgatctg catttcctgc
    18481 tccgtgtgcg atccagacac cgccggcagc gcgcatgatc ggatccaggg ccgccgtcat
    18541 cccgctggcc ggctgaatac actcgatctc ccggccggaa agacggtgaa gatatggctc
    18601 acggttggaa acgacaacaa actgaaggcc ttgcagccgc aaggcaatca gctcatgtaa
    18661 gtcttgttta gtccacacta tcgctccact tgccagaggg ctcttttcta tttttgagga
    18721 tgaccggaca ggagaaccat cccctgatcg gtgtagttga agcctggaaa cagggtacga
    18781 gagaaaccta agtctcctga tgtgaggaaa tctgagctat taccgcattg ggctgcgctc
    18841 agtggacttt cgagcgatca cttcaaagct gctcgcgaaa cgtcgaaaca gaggaattcg
    18901 aagaaacact tcgtcaatcg cccgcaccac ccggtagcca gaacgccatc ccggcagcag
    18961 tccgccgagg ggggttgaca gcatgtcgta gtactcatgc cgcgcatcga gacctgcttg
    19021 acggaacaag tcggcagtcg gtgatggcgg caactcccgc tcatccggcg tttgatactt
    19081 ccgcattaga tggggaacaa gcagcttgcc aagcgcgcca gacagccggt agcgatttgg
    19141 atcgagcgaa tagaaccagc cgcccggacg gaggcgtccg gcaatcgtcc tcgccgtccc
    19201 gggaaggctc tcatccggaa ggtggtgcag gaagaagatc gcgtagataa aatcgaactc
    19261 accctccaca tcgtcaatcg tgccgtggag gaaccgcgcg ttcgtgaggc ctgcagttgc
    19321 ggcatcggct tgcgcctgac ggattcccgc gggcgacaaa tccacaccga tcaattcgcc
    19381 gacgtgcgga gccagcagaa gttccgtgtc tccgatcccg cacccgaggc tcagcacgcg
    19441 cgattcaagt ccaaccggac acatcgacag catgcgccca acgaggtggg cccggagttc
    19501 acgaactgcc gctttgctga agtgcttctg agcaaaaccg gaatacagcc gctcgtgata
    19561 ctcgacttcg cgttgcagaa cgtctcgcgg cacattcatc ggattggtcg tccaatttta
    19621 tccggaacgc aggcgatcaa ggatatcgac aatcgggccg agcctgggcc ctgccttcac
    19681 gctacgagaa atcgggttgt cagtccaatc ccataaccgg ctgggccgct ctccgatgtt
    19741 cactggcttc tcgtcccaca tggacgctga gtagcagaag gctcctactg cctgcacgaa
    19801 gatggagaag gtcagcagaa ccacaaacac cgcccgcagg gtacgcgaac gaaagatcgt
    19861 ctccgtcgcc ggcgccatta ggaccacgaa cgccggcagc gcgtcggaga gcagcctggg
    19921 accgtagcag tgaccgcccc accagacacg ccatgttgcg atcagaacaa cctgggacac
    19981 agcaaacacc agcgccgcca gataaacggg ttgagcgggc catttccgtc gcctgaacaa
    20041 aaagaacacg ccggccacaa agaacagtag gacaggggag tacacgaaga ggccccttgc
    20101 gggactgaag agcacgccgg cgagcccgcg ccaaagattc ccgtcaaagg acagagcagg
    20161 tgcgccctga acggcaccga acacgccgac gttataggcg accgtgacca caccagccaa
    20221 gaccggaaac agccagaagg ccgccattaa cctgacccgg cgcgacgagg caagcagccc
    20281 ggtgaatgcg gcggcaacga acagaatgtt cgtgggccgg atggcggcgc tcagaccggc
    20341 gaacagtccc gtcagcaacg cagcctgtcg gtcgtccgca cactcacgga gtctgtccaa
    20401 gaaaaacagc gccaggatga tcgcgacctg gctgccgccg tgttgccaca gggcctggct
    20461 ggagattgtc caggtagcag taccgaaacc gtagagaatc gcgagccagc gggctcgctt
    20521 ttcgtcagcc agcctgactg ccaggaggta aaaaagcgcg acagagccgg cggcaatcag
    20581 cgacgcggtg agcttctcca gcaccccagc gagaaccatg acctgcgccg gactccagcc
    20641 ggcggccgtc acatataccg ccgcggggac gtaaagcgga gtgagcaata tcggcagtgc
    20701 aatcgggtac tccgagtacg cgtgcccgct cacaatcggc agggcgcgca aatgttgagg
    20761 tacgtgctcg cggaccgccg gataaaagaa gtccaggttg actgtgcggt cgagcaatat
    20821 tgaaaacggc aacagcgaag cggggagcgt gtcgcccgag gcgatgcgct ggaagttcag
    20881 attatagaca acgagcgagc aggcaaaaag cagcagcgta aatcgcgccg gccgatgccc
    20941 ggtgtcatcg gatgcctgtt tcaatatgga ccagttccga ttcccgtgat tcgctctccg
    21001 tggcgggcgt agccgcgatc gtctggggcg cgcgccagaa gtccatatat gatacctggt
    21061 gcacacagga cacggtgcag aagggagcgc acggtttctc ggtgaggaac tcccggcgga
    21121 tgtcgtcctt cgtgtactca gccagcggtt tcgccggata gccgcgctgc tgggagcaat
    21181 agtggacgag gccgtcctcg cacacgtaca ggtagcgcga gccggcgcgg catcgccagt
    21241 tgctcggacg gccgttcgcg atgttgttct ggaagtaatt gatctgcgag tagctggatt
    21301 tttccagccc tttcatcgcg aggaacactt cacgctcctg gtcgccgagc ggcctgagct
    21361 gccccgagcc gtcgtggatg atgccgaccg tcgaagtgaa gcccagttcg accgcacgcc
    21421 gtccaacggt aagtgcgtcc tggggattgc ggatgccacc cccgaccacg gagttgatgt
    21481 tgacgtgaaa ttcggcgtgc ccggcaagaa gctgaagttt cttgtccaat accttgaggc
    21541 tcttcttcga gacgtcgtcg ggtgtcacgt tgtcgatgct gatctgaaga tgatcgagcc
    21601 ccgcccggtt cagccggtga attcgcgccg ctgtcagcag gtagccgttc gtgatcaggc
    21661 cggcgatgat cccccggcgg tggatgtggg cgatgatctc gtcaaggtcg gggtgcaaca
    21721 acggttctcc accgctcagc gttatgaccg tcgtgccaag ttcgcgcagg cgatctacgc
    21781 gctgaagcat cgtctcggtt ggcagcggct tcgacacgtc atcgtactcg ttgcaatagg
    21841 cacacgaaag gttgcagcgg cggatcggga tgagatgcgc caggaccgga tggtcggtag
    21901 acaccagtcc cttgaaaatc atttgcagtt ctcgcgctct ccggcctgcc gcacgcagac
    21961 ggcgcttcaa gcgtagggcc ttatcaggca tagagttaca gggctttctc tattacagca
    22021 cagggttgcc ccggaggcca aagctaccct gtttccggtc tgttttcccg cccgaacggt
    22081 gaaaaatcag ggtttcttgg cagcctccgg cgccggaggg gtgggaggag ccggaggcgt
    22141 gggctttacc tcctcaccgt acttgatctg cacctcgctc ttgaactgct tgtagtcctc
    22201 gtagcggacg gtcatccgga tgggcacgcg catcccgctc tcgaactgca acacgtcgtt
    22261 ggagatcgtg tacgtcggaa accagtactt gccgtcaatt tgctcgcggt aggtttcgaa
    22321 tttcggaaac tggttgtcgg atccccgttt caggagcccg acgccccgcc catacgtttt
    22381 cacaatctgg aggtcacggt catccaccca gatctggccg gagaagtacc gctgtccctg
    22441 ctccatcttt ttcggtttca ccgcaaagag atagcaggga atctcgtcgg ccatctgctt
    22501 gcccaagtag cgaatgtcgt actttggcag ttccttggta gtcagcacaa acggctggac
    22561 attccgcaaa tcctgctcat cttccggggt caatagcaga ttctggagag tggaaacagg
    22621 cgcgcggacc acccgttcgg tccgcttgcc gtcggacgag aagatgatgt ccgacaccat
    22681 ctcatgccgc ccgcggaccc tgcccgaatc gtcgagctct tgaatccgag ccgtctggcg
    22741 gtacgtgtag ttctcgcgcg cgagcgcgaa ttccgcctcc ttggcggcaa acttgttgat
    22801 gatttcgtcc gtctccgccg gagtgagctc ctctttgccg gccctcacag cggtaaacgc
    22861 cattgcagtg atcagaaaca gccgtaatgc gcgcagcata cccctctcct cttgtgcgac
    22921 gaacgagcag ccattaaggt ttcccgagga tttggcgggc gatcgtcatc agttgcatat
    22981 tgctcgttcc ttcgtagatg cgcccgatct tggcatcgcg gtagaacttc tctgccgggc
    23041 agtccttcgt gaaacccact cctccgaata cctcgaccgc cttggacgcg accgtctccg
    23101 cgatctccga gctgtagtac tttgccatcg ccgcctccgt aagaaacggc atcccggcat
    23161 cccgcaaccg ggcggcattg tacgtcagca atctggcagc ctccagttcg gttgccatcc
    23221 tggcaaggtc gaactgaatg ccctggaact ccgcgatcgg cttgccgaac tgtttccgct
    23281 gccgggcata cgaaagtgct tgctcaagtg ctccgcgagc caggccgatc atctgtgcgc
    23341 tgatgccgat ccggccttca ttcagcgtct cgatggcgat cttgtaacct ttgccgagct
    23401 cccccatcag gttcgatttc ggcacgcggc agttgtccag aatcagttcg caggttgagg
    23461 aagcacggat gccgagtttg tcttccttct ttccgacctc gaagccggga gaattgcgct
    23521 cgaccaggaa acacgtgatg ccgcggtggc cggcttcggg gttcgcattg gcgaatacca
    23581 ggaagaggcc cgcctccgcg ccattggtgg tccagagttt ccgtccggac agcagaaagt
    23641 gatccccttg atcggcagcc cgggcggcca aggcgaaggc atccgacccg gcccctgcct
    23701 cggaaagcgc gtacgcccca accgtatccg cagcgagctt cgggaggtac cgctttcttt
    23761 gttcgtcatt tccccagcgc aacagggcgt tgttgaccag agtgttctgg acgtcaacga
    23821 tcacggccgc cgcggggtct accgccgcga tttcctcgac cgcgagcacc gactggaaga
    23881 acgttccgcc ctgtcctccg tgttcctccg gaatttcgat gcccatcaac ccgagttcaa
    23941 agaattcgcg caggatctct ttgcggaaga ctccggcttc gtccatggcc cggacgtgcg
    24001 ggctgacctg ctccttcgcg aactgccgca ccgacgcctg gaagagcttt tcatcctcgc
    24061 tgagctggct aagcggccgg gccggcccct cggcgactgt cgacatgctt tcagtctaac
    24121 gcctgacggc tggattgctt cggcctgcag tagaataggc atctcatgaa gaattggaaa
    24181 ctgatcgctc agggattgga cgtcgccatc cccgaggccg accttccgaa actctcacaa
    24241 accctcgagg cgatggacac cgctttccgg ccgctggccg aggcgatccc cgacgagatg
    24301 gaaccggccg tcattttcca cattcagccg gaggagcagg tatgaccatc ctcgaggctg
    24361 ccgccgcaat acgggcaaag aaagtaacgt ccgccgagct aaccgcggaa tcgctctcca
    24421 gcatcgcgcg gttgcagccg cggctcaacg ccttcattac tgtcgccgag gagagagcac
    24481 gcgagcgcgc acgccgcgcc gacgaggaac tcaagcgcgg gatggaccgc ggcccgctgc
    24541 acggaattcc aatcgccgtg aaagacgtct tctgcatgag aggaatgcgg acaacttgcg
    24601 gctcaaagct gttcgagaat cacgtgccgg accgcgacgc tgctgccgtc gaaaggctgg
    24661 aagccgcggg cgccgtgatc accggcaagg cgaacatgca tgagcttgct tacggcatca
    24721 cctcgagcaa cccgcatttc ggtttcgtgc gaaacgcgcg gaacccggag cacatccctg
    24781 gcggttcgag tggaggctcc ggttcggcgg tcgccgcagg aatggtgttc atggcgatgg
    24841 gcagtgacac gggcggatcg attcgcatcc cggcgtcctt ctgcgggacc gttggaatca
    24901 agccgacttt tgggcgcgtc agccggtatg gcgtcatgcc gctcgacttc agcctggacc
    24961 acatgggtcc gttgacgcgc accgtccgtg acgccgcggt gtgcctcggc gccatcgccg
    25021 gccacgatcc gcgggatgag acctccagcc gtcagccggt tcctgagttt ctcccgccca
    25081 ggcaggtttc gattcagggc ctccggattg gaattccgga gaacttctac ttcgagcggg
    25141 tcgatccgga ggtggaagcc gcggtccgcc gtatggcgtc cgccgccgcg aaccttggcg
    25201 ccacactcgt gccggttcgg gtgcctgaca tccgtgagct caatgtgatt gcacgcgtga
    25261 tcctactcgc cgaggcgtcg gcggtaatgg agaagcatct ctccaggcgg gagaacttcg
    25321 gcgccgacgt tctgtcgctg ctcgatcagg gccggctcat tgcggcgacg gactacgtga
    25381 acgcgcagcg cctgcgtcga gtgaaacagc aggagttccg gcgcatttgg cggcacgccg
    25441 actgcctgct cactccgtgc actccgaccc ctgcgccgaa gattgggcag accgcaatca
    25501 cgatcgccgg agaggcagag gatgcccggc tcgcctccac gcgactggtg cgggcgatca
    25561 atctgctcgg gctgccggcg ctggcgctgc catgcggggt gcacacgttg gggttgccga
    25621 tgggaacgca actgatcggg cggccattca ccgaaccgct cttgttccgt gtgggggccg
    25681 cactcgaaga cgtgaccggc ttcgacaagc gcgtgccccc ggagtgctaa tctgacctct
    25741 ggcgcgtgta ccgccgtgct gacacgcgcc acaggtaaca aaccaggaga tctccatgca
    25801 gttaagccgt cgtaacttcc tacagacagg cgccgcgctc gccgccgcgc cagccacgtc
    25861 cagatcgtac gccgccaacg accgagtcca gttcggattc atcggcgtag gcgcccgcgc
    25921 tcatcaactc atcccctgcc tgctcgacaa cccgggcgca gagatagtgg ccgtggtcga
    25981 cgcgtatagg ggacgcgtcg ggcgcgccat cgaacgcacc agcagtcgcg ccaaggcagt
    26041 caaggactac cgcgagatca tcgctgacaa gtccatcgac gcggtcgtcg ttgcaacacc
    26101 cgatcactgg cataagcaga tggtgatcga ggccctccgg gcgggcaagg acgtctattg
    26161 cgagaagccg ctgacctacc ggtcctccga gggtgtagag atcatccagg ccgcgaagga
    26221 aaccggacgg atgcttcaag tcggcagcca gggcatgagc tccgagaccg agcgaaaggc
    26281 gaaggagatc atcaagtccg gccggctggg caaaatcacg atgatccgtg cggcgtacaa
    26341 ccgcaacacg ccgtccggcg cgtggattta tcccatcccc ccggacgcct cgccggagac
    26401 agtgaattgg gaaatgttcc tcggatcggc gccgaaacgg ccgttcaatc tcgagcggtt
    26461 tttccgttgg cgctgtttct gggattactc cggcggcatt gcgaccgacc tgttcgtgca
    26521 cctttgcaca accattcatt tcctgatgga agcgaagatg ccctccaagg cggctgcgct
    26581 tgcccaactc taccgctgga aagagacgcg cgaagtaccg gacaccgtca acggcgtgct
    26641 cgagtacccg gacggcttct cggtgaacct cagcggcacg tttaacaacg aagctgccgc
    26701 ggagtctaac ttccagatcc ttggtaccga gggcactctg tccatcggtt tcgccgacct
    26761 gagtttccgt cccgaacggg tgaatgatga taaccgctgg atcgtcgaca gttggtcgca
    26821 ggagctcgaa gacgcctact accgcgatcc gaagatccag gcgcgggaag tgccaagcag
    26881 caggaaggcg cgtatcctac cgggcagcga atcgtaccgc gaggaaggca aggattccac
    26941 agtggctcac ttcgggcact tcctcgagtc gatccggacc cgcgagccgt actggcagga
    27001 cgcaaccgcc ggtcatcatg cggccgcctg tgcccacatg gtgaacctct cttcccgtga
    27061 gggccgcgtc gttgagtggg agttcggaaa ggacgacatc aagaaggcgt agaacgtgct
    27121 gcgcccctaa actccgcgct gtgcggctcc gatttcaata cagggagccg tgtacattcg
    27181 tcgcgaagtc ggccgcctgc ttccgcacgc cgcgccgtca ttgtcggatt tcctgttcgt
    27241 gtccctgctc gtgtggctgt tcgccaccgg gagtgggtgg gaagccttgc tggccgacgg
    27301 cgacaccggt tggcacattc gtaccggcga ttacgttctc aatacgggtt ccatacctac
    27361 gaccgacctg ttctcgtttt cgcggccggg agagccatgg ttcgcctggg agtggctcgc
    27421 cgatgtcatc tttgctctgc tccacgaccg gtgggatctt gtcggcgtgg tcgtggtagc
    27481 cggggtcgtg atcgcgctca ctgctgtcgt cctcttcctt cacatgctct ggaggggcgc
    27541 caatgtcttc gcggccctgg ccgtcgtgtt gccttgcgtg ggtgcggcgt ccattcattt
    27601 cctggctcgg ccgcatattt tcacccttct tctgctcgcc gtgtcgctat gggtgctgga
    27661 ccgggaccgt cgcgcgccag gggtctctgt ctggattctc gtgccgctgt cagccctctg
    27721 ggcgaatctt cacggcgggt ttctcgctct tctggcctgt gtcggattgc tcgcggcggg
    27781 ctgcgcggct gaggcgcttc tcgtctccgg cagatgctgg actgcggcgc gccgttacgc
    27841 ggccctgacc ggcgcctgct ctctggcaac cctgctgaac ccgttcggtt accgactgca
    27901 tctgcacatc gcgcagtatc tccgctcgga ctggatcaag gaggcggtgg acgagttcca
    27961 gtcgccgaag ttccgatcgg agagtctgct tcaattcgag ctgctgctgc tggtgagcct
    28021 gctgcttgcc ggcatcctgc tttcgaaacg gcgcatcgcg gacgcgctgc cggtgctgtt
    28081 ctgggcgcat ctttcactca gctcggtccg ccacgttccc gtgtttgtga tcgtcgtggg
    28141 gccgttgatc gcccgggagt tgacctgcct ttgggaaagc tggaccgcca cggctccaac
    28201 gagatcgctg gccgggattc tgaacgccgt ggcacaagac ctcgcacccg gtttccgaag
    28261 ggtgagcccc tggtcggtgg cgctggtgct ggccgtgctc tggctgacgc cgtcttcccg
    28321 atggccgcgg gactttccgg cggctaagtt cccggtcggt cttgtcggtg cgaaccgcga
    28381 ccggctggct tcggctagga tcttcacctc agatgagtgg gcggattacc tgatttaccg
    28441 cggctggccc cgccagcggg tgtttctgga cgggcggagc gacttctacg gaccggtcct
    28501 cggcaaggaa tacctgcggc tcctcgaagg tcgtaccggc tggcgggatt cattgaaaaa
    28561 acacgggttc cggttcgtcc tgttgccgga agacgcaccg ctcgccagcc atctccatgc
    28621 gtgcggagac tggagacaga ctgctgcgga ccgtcaggcc gtgctgttcg aacggatctc
    28681 ttcagccccc gtgaggtccc taatcaaagg gcgatgctcc accgatagtt ccgtgttgga
    28741 agcctcacgg catgaaatcc aaagagaggg acacccagcg acaacgacct tgtccgtccc
    28801 gggcggattc cgcggcggcc tcgcaagagc tcggtgatcc ggcggaactg cctgcctggc
    28861 gcgtgccctc cggacctgcc ctgggtgaat tcgccctgcg gtccggtttc atcgccggaa
    28921 ggcttttgaa gcccgcaggg aactgagcgg ccgccaaggc agaggggagg gaaacagatg
    28981 gatcgccggt ttctgaccgt cttaggggtg agcctcctgt tcgccctggt agtcgctagc
    29041 gtgttttacc agatgaacgc gcggttccgg ggctcagctc gtaaacggga caagccgaag
    29101 caggccgagt tggtcgtggc ggctgcctca ctgccggttg gcgtgaccat taagcccgcc
    29161 gacgtcaagg tagtcaaggt cgaaggagag cgttttccga agggcgcctt cgcgaaagtg
    29221 gaagacgtgt tgggccggcc ggtggtaagc aacatcctgg ctgaagaacc ggttatggag
    29281 ggacggctcg cggaacgagg cagcggcgtg ggcttggcgc cggttatccc ggtgggtatg
    29341 cgtgcggtct ccgttaaggt gaacgaggtc gtcggggtgg caggctttgt tctgcccggg
    29401 atgcgcgtcg atgttctggc gaccttgcgg ccccagagcg gagatggtgc acgcacctca
    29461 accgtcttgc agaacatcct cgttctctcg gcgggacaaa atattcagac tgacagccgc
    29521 ggccaggctg tcaacgttcc agtggtcacc ctgatggtct cacccgaaca ggccgaaact
    29581 ctgacgctgg ccggcaacga ggggcgtatc caacttgtgc tgcgcaatgg cgcggaccaa
    29641 acggtccagc cgacgccagg caaggatctt acagaactgt acggccaccg gaagccgcaa
    29701 ctcgccgagg tatccgaagc acggccaagg ccacaacccc agccgcgcgt cgcgccggcg
    29761 ccgttccctc ccccagcgcc gccggaaccg atccgcgacg agatcgttgt gattcgcgga
    29821 acgcagaaaa gtctcgaggt gatcggtgaa acgaaatcca actagccctc ccaggccgga
    29881 agcgaggcgg cggcgccttt tcttgctgat gttgggtctc ctggctgcga tcggcacggc
    29941 atctccggct cagcctccgg cagaggacct cgcgctggcc atcggcggca gcgccgtcat
    30001 cgactatccg gaagacatcg gccgcctcgc taccagcaat ccagaggttg ttgatgcgtt
    30061 agcggtaacc aaacgcgagg tgcttctcca ggcgaagtcg cacggcatgg caacgatcgt
    30121 catctggtcc aagtccggcc ggagggattt ttaccgggcg accgtttcct acaacctgga
    30181 accggtccgt aagctcctgc gcgatacatt tcccggagag gagatccagg ttcaggcggc
    30241 gaaggattcg ctctcgctaa cgggcagggt gagcagccag gcggtggctg agcgtgccgg
    30301 cacgctcgta acaccactcg caaaaagtgt agtcaacaat ctcgatgttt cgagctctgc
    30361 tgagaagcag attctgctgc gagtgcgttt cgccgagctt gaccacactg cgtcggccag
    30421 cttcggggca agtctcattt caaccggggc tttgaatacc cccggccgga tcacaaccgg
    30481 gcagttctcc gccccagcgc cgcagaagat ctttccgccg ggacacgatc tctccggggt
    30541 caccggcgtc attccgggcg gcaaccctgg gaccttcagc aacttctcga ttggagacgc
    30601 gctgaacatc ttcgcgttcc gccccgacct gaatctcgga gcggtgatcc aggcgctgcg
    30661 gaaccgaggc ctgttgcaga tcctggcgga accaaacctg gtgacgacaa acaacaagga
    30721 agcaagcttt ctggtgggcg gcgagtttcc ggtgccgatc gtccaggggg gcgccaacgc
    30781 aggtgcggta acggtgcaat tccgcgagtt tggcattcgg ctcgcgtttc tcccggcggt
    30841 gaccgggcac gacacgatca agcttcatgt gaagccggag gtgtcgtcgc tcgacttcgc
    30901 caacggcatt tcggtttccg gtttcagcat ccctgccctg tccacccgcc gcatggagac
    30961 aaacatcgag ctcgggctgg gacagagctt cgtgatcgcc ggcctgatcg atgaccgcgc
    31021 gacggaaaat ctggcgaaga ttcccggcct ttcgcagatc ccggttctgg gggcgctctt
    31081 caagagccgc tccgagaaaa agacgaagac cgagctgatc gtgctggtca cgccggagat
    31141 caccggcccg gtagatgcgt cgcgggtccc ctccggcccc acgatgccgc tagacttcat
    31201 tccgaaagtg gaccggccgc cggcggcccg cgaagaaggc cggaagtaag aggcgaggaa
    31261 tgggccggcc gggcaaatgg cgaagcgcgg agttgtcggc ggtgttgatt gcaccggacc
    31321 gggaactggc ggctcaattc tgccggaccc tggagaccac gcgtgcgttt caaatcgtcg
    31381 ccgagctgaa gaaatacccg agtgaacaaa cgctcgatat ccgcctgcgg caactccggc
    31441 cggacgcagt actggtcgat ctcgccagca acttcgagac ggccgccggc ctgctgcggg
    31501 ctctggcggc gtttcggcct gccgttcatg tgatcggact gcatcgaact aacgatccgg
    31561 acgtggtgtt gcgggcgctg caactcgggg cgagcgagtt cttccatgcg ccgtttgacc
    31621 cggccgttca gcgggacgcc gtcgccggct tcctgaggct gcgccgcgtc gaggtccagg
    31681 ccggcgttcc accgggaaag gtcgtggtgt tctccagcgc caagcccggc tccggcgcat
    31741 ccgtgctggc ctgccagacg gcgctggccc tacgcgaggc gacgggcgag cggatcctgc
    31801 tcgcggatct cgacctgaca ggcggcaccg tgggcttcta cctgaaagtg aagtgcgcgc
    31861 actcgctgat cgacgcggtg gagcgatgcg aacaccttga accggcggca tgggccgcgc
    31921 tgaccaccac gtgcgatggt ctcgatgtgc tgccggcccc ggctgttcct cgtgaagcga
    31981 acccagacaa cgaccgcctg agagcggtgc tcgatcagtc gcgccacctc tacgactggg
    32041 tcctgctcga cctgccggcg atcttccacc cggtgagcct gaccgcgtta gtcgaatgcg
    32101 acagcgcgtt tctcgtctcg accgcggaac tgcccagcct gcatcttgcc cggaaggccg
    32161 tgaccctgct cgcacagttg ggaatcggca aggaacggct gaacgttcta gtgaaccaag
    32221 caggccgggg agacggcatg agcggatcgg acttggaaaa gatctttggc tgcgccgtcc
    32281 attcgtcctt tcctagcgat tattcctcgc ttcataaggt ggtgactctc ggggaagctg
    32341 tggctccgga ttcgaatctc gggaaggcgg tgggagagtt tgccagccgg ttggcaggcc
    32401 tcgcccggag tgagaagcgg cgggcaggca tggtgttcaa ttcagacccg gccttcgccg
    32461 gaacgtaagt cggagcgacg ggaaacagta tgttgacaca cggggacaca aaagcttcgg
    32521 cggaggttac ctgggagcag cggctgcttc ggaactctgc ccgccccaaa accgccctga
    32581 agcctgaatg tcaggaactg aaattcatcc tccaccgaaa gctgctcgag aagatcaatc
    32641 tcgaggcgct ggcaaccatc gagaaccagc gtgtgcgcgg cgaggtccgg caggcgctca
    32701 tcacgctgat ggagggcgag cccacgctgt tgagctcact ggagaaacaa cagatcgtcg
    32761 aggaagtgct cgacgaggtc ttcggactcg gtccgcttga ggcgctgctt caggacacaa
    32821 ccatctccga catcctcgtg aacacccacc gggcggtctt cgtcgagcgg cgcggtcttc
    32881 tggagccgac caacgtcagt tttcgagacg acaaccactt gcttcacatc atcgacaaga
    32941 tcgtctcgca ggtaggtcgc cggattgacg aatcgactcc gatggtggat gcgcggctca
    33001 gcgacggctc acgcgtgaac gcgatcatcc cgccgctcgc tgtagacggg ccgctgttgt
    33061 cgattcgccg tttctcggtc gacaaactga tgccgcggga cttggtggag cgcaaggcga
    33121 tgacgccggg catgatggat ctccttgaag cggccgtaaa ggcccggttg aacatcatca
    33181 tcgccggggg gaccggcgcc ggaaagacca cgctgctgaa cgcgctgtcg gccttcatcg
    33241 ccccccggga gcgtatcgtc accatcgaag acgcggccga acttcaattg aaacagccgc
    33301 atgtctgccg gttggaaacc aggccagcca acctggaggg acaaggagcg gtccgccagc
    33361 gggagttgct ggtcaacagt ctccgcatgc ggccggaccg tgtcgtcgtc ggcgaggtcc
    33421 gcggtgcgga ggctctcgac atgcttcagg cgatgaacac aggtcacgac ggatcgctga
    33481 cgacggtcca cgccaacagt ccgcgcgacg cgatctcgcg cctcgaggtg atggtctcgc
    33541 tggcaaacgc aaacatgcag ttgagtgcca ttcgccaaca gatcgcgagc gcggtacatc
    33601 tgttcgtcca ggcggcgcgt ctgagcgacg gctctcgccg tgtagttaac gtcaccgaag
    33661 tgaccggcat ggagggcgag gtcattacac tccaggacat cttcgtcttc gagaagcgcg
    33721 gcctgtcacc cgagggcagg gtcatcggca gattcgccgc gactggcatc cgcccgcggt
    33781 tttacgagaa gtttcttgcc gccggcatcc aacttcgccc ggacctgttc gacgagcagt
    33841 tggagatctg aggaggtagg aggtggcgtt cttctggatc ctgtttgtca ccctctggct
    33901 gctggcgatt gcctgctggt ggtgtgcctc gagggtgctg cggaattccg atcgcagccg
    33961 catccgcggc cgtttgctcg gcacgccaag ctccagcacc ggcaagaagg agggttcgcc
    34021 ggcgccggtg ctgattccga ccgaggacaa ggatacgggg aagatcgtcc tgcggctgct
    34081 tgaccgcttc cggttgaacg tccgcctcca ggaactcctg gagcagtccg gcctcaagtg
    34141 gcacgtcgcc aggctgacgc acgcatgcct ggcgctggcg cttgccggat tcgcaatcgg
    34201 cgggtggacg gcgcccccag gccacgcgga gattccggtc ctgctcgcgt gcgctggtgg
    34261 cgcgcttccg cttctgctgg tgtcgcggaa gcgaaggaaa cggctacgcc gctttgagga
    34321 acagtttccg gacagcctgg aattcgtagc acgatccatg cgtgccgggc atgcgttctc
    34381 ggtgtcgctc gaaatgttgc accgcgagtt tcaggatcca ttggcctcgg agttccaccg
    34441 cacgttcgac gaacacaatc ttggcctgcc gctcgacgcc gcactcgaaa aactggcgcg
    34501 gcgagtgccg ctgatggatg ttcacttctt cgtgtcggcg gtgctgctgc aaaagcggac
    34561 gggtggcaac ctagccgaac tgctcgacaa gctggcctac gtcattcgtg agcggtttaa
    34621 gcttcgtggc cgcatccggg cggtcagcgc gcacggccgg atgacgggtg cggcgctgtc
    34681 cacaatcccg gtgggagtgg cgggaatgat gttcttcgtc aaccgggagt acgtcacctt
    34741 tttcgccaac gacccgaccg gccagtggat ggccgggggc gcggtggcgc ttcagttgat
    34801 cggctacgga atcatcagaa agatcgtgtc catcgaggta tgaggcgtgg aacaagtcag
    34861 caaaaccggc ggacggcacc gttgcccggc ggcgagaagg tttcggaacg gatgtgagaa
    34921 atggaactgc tgctgactgg gatcctgttc gtggttgtca tgggcgccgt ctcggcggcg
    34981 ggagtacggc tctgggttag gccgaaagcg gcgatggagc gtgtcaccgg agcgattgtc
    35041 ggcgcagaac gcggtgtccc ggttcatccg agcttagtct ttcgggatct gatccaccga
    35101 atgggcgcgc tgcttccagc gtccagccgg gaggcagggc tgacacaacg gcggatgatt
    35161 cgagctggct tccggaatcc cggcgcggtc aagatcctct acggcgccaa gatctttctc
    35221 ggagccgttc tcccactcgc tgccggcctc gctgtcgccg ggtgggaggg gccgtcggat
    35281 accaagatgg tcgtcgtcgc cggcgcagcg gcggctgggt ttatggctcc cggatactgc
    35341 ttgaagctgg tggtaaagcg aaggcagaag cgtatccggc gcgggctgcc ggacgcgttg
    35401 gacctgctgg tgatctgtgt cgaatcgggg ctggggctcg accaggccat cctccaggcc
    35461 gccaaggaac tcgaaatctc ccatcccgcc atcagcgagg aattcgcgct cgtcaacctc
    35521 gagctgaaag ccggcaagcg gcgtgccgaa gcgctgcgaa acttggccga ccggacgtcg
    35581 gtggacgaac tcaagaagct cgttgctgtc ctcatccagg cggaccggtt cggcacgagc
    35641 atttcgcaga gcctgcgtac gcattcggac ttcatgcgcg tgcaggcgcg ccagcaggcg
    35701 gaggagaagg cggccaagct cggcgtgaag ctggtgttcc cgatcttctt ctgtatcctg
    35761 ccgacgctct ttgtagtgac tgtcggcccg gtggcggtgc ggatcgtccg tgacctgctc
    35821 ccgatgatga acaacatgta gccgcaaggc gcggcgactt tggaaaggag aaacgcaatg
    35881 gactctttca cgattcggat catttgcacc gtgctcgcgg tgctgctgct aggcgtaatt
    35941 gtccttcggc gtcgcaagag cgccgaataa acggccaggg accggatacc cgggccaaca
    36001 ctgaggtcgc accgacgagc gtcgccaccg ctggctgtgg gtcgcgccgc agtccagcag
    36061 gggcgggacc gagggtgcat gtaaggagga gtacgtgatg aaaagggaag catggtggag
    36121 ccttgttctg tgcggctgcc tggcggcgcc ggtatgcggg ggcacattcg ggaaggctgt
    36181 ctcgatcgga gggcacgcgt ccgatctcgt cctcgacgaa acgcgcggcg agctctacgt
    36241 cgcgaatttc acagcgaacc ggatcgaggc actggcggtc cccgatggca cactgaaaca
    36301 ctcgatcaac gtcgcgccgc agcccggctc gctcgccctc tcgcccgacg gccgctacct
    36361 gctcgtcacg cactacgcca actaccagac ccccggcatg ccccgtaacg ccattaccct
    36421 gctcgatctc gcgaccggcg ccaggcagac tttcgcgctc tcggcgccgc cgctcggggc
    36481 tgccttcctg atcaacggcc gcgcgctgat cgtaaccacc acggaattcc tctcgtttga
    36541 gccggtctcc ggcaccgtcc ggctgctcga tacggtggaa ggagtcacgg ccacaacgct
    36601 cccggcgccg ccggccaact ttcccccaca gatcatcgcc gcctcggtgg ccgcctcgcg
    36661 ggaccaacag gtggtctacg gccttaccga cactatccgc ttccgctacg atctactcag
    36721 cggccgcgtc gtctccctcg gctacaccgc ggagccgccg ctcgggccgc gcgtggtcag
    36781 cgtcagccgg gatggcgcgt attacaccgc cggatgggcg ctctttgacc gtcagggctc
    36841 cgtgatcgct cagtttcccg gcgcttccgg gcttctcaat gtcggcagcc acgccatcga
    36901 ctcggaccgc ggagtgattt acgctcagat cccggcagaa gagggcgcgc caccggtctt
    36961 gatgatcgcc gacgcggaca atctcacggt gcgcgaacgg gtccaactgc ctgaaaacct
    37021 cgccggccgc agcgtgctta gctccgacgg ttccatgatg tattcggctt ccgacagcgg
    37081 ggtgctcatt ctgccggtgg gaaactacag ccgcgcccgc cgcttgaaaa cctcgtcgga
    37141 ggagatcttc ctacggacag gattctgcaa ccgccgggtg gcggttcagg gtctctggat
    37201 cgaagacgaa agcggcgcca ccactgactt cacgatcgcc tcaagccatc ccgcaatcac
    37261 cgtatcgccg tcttacggaa ccacgccggc ccgggtggag gtccgggctg atccgaacgc
    37321 ccttcagaac cgatctggca cggctgtggc ctggctggac atccgctcgt ccggggcggt
    37381 gaatctgccg tcccgtgtac gcgtgctggc cagtctcctc gaacctgacc agcgtggcac
    37441 cgtgatccac atcccgggac gcctggtcga cattctcgcc gaccctgttc gcaatcgctt
    37501 ctatgtgctg cgacaggacc ggaacgaggt tctggtcttc gacgccgccg actaccgccg
    37561 gatcgccgtc ctccgaaccg gaaacacgcc cacgcaaatg gcgatcaccg ccgaccgccg
    37621 tctcctgctc gtcggcaacg ataactccca aattgcgaat gtctacgatc tcgataccct
    37681 ggtacagacc gctccggtac gcttcccgcc cggtcattac ccacggtctc tggcggcggc
    37741 cgctggagct attctagccg cctcccgcgt ggccggcccg aaccacacga tcgatcgcgt
    37801 cgacttgctc acccgcaccg ccagcgaatt gcccacactc ggcgtttacg agaacaaggt
    37861 cgatctccac acagttttgg tagcctccgg caacggctcc tccatcctgg tcgccgaggc
    37921 ggacggcagc ctcatgcttt acgacgccaa cgccgccaca ttcaccatct cacggaagga
    37981 ctacacgtct ctcgaaggcg cgtatgccgc atcgaacagc ggcatgttcg tggtcgataa
    38041 ccttttactg aactcgtctc ttgtcccggt cgggcgcttc gagaagggag cgaacctttc
    38101 ctcgggcttc gccttcgacg gcgattccgg cttgctgacc ctcgctcccc ctggaggagg
    38161 cgccggggta atggaacgcg tcgacctctc ctcggcggcc gctgtccggc cgaccaggat
    38221 cgccgaagcg ccaccggagc gtgaaccagg ctttgcgctg actcgcacgc tcgcgccgct
    38281 ggccaaccgc caggcgatcg tctcgcttac gaccgccggg tttaccgtgc tttcctggaa
    38341 ctacgatgcc gcaaccgcgc cgcccagaat cgacgccgtc gtcaacgccg ccgacttcag
    38401 ccggccagtg gcccccggcg gactgatcgc cgtattcggt gaggacctga gcccggtgaa
    38461 tatggccacg agcgagatgc cgcttcccac cgccctcggc gacacgtgcc tcaccgtcaa
    38521 cggagcacct gtaccaatgc tgttcgtgtc acccggacag gtgaacgccc agttgccgtt
    38581 tgaggccgag ggaaacgtta cgctggccct gcacacgccg ggaggcgtca gcgacaactt
    38641 tccgctgagt gtgtcgtcca ctgctccgag cctgttccac agcggatctg ctggaccgtt
    38701 ggtagacata gcgaccgtcg tgcgcgccga gaacaaccag ctcgccactc aagccaatcc
    38761 cgttcacggc ggcgacgccc tcgtgatcta cctcaccgga cttggccgcg tttggccggc
    38821 cgttcccgcc ggctctgccg caccgtacga tcccctcgca caggttgtga tcccgcctgc
    38881 ggtcacgctc ggcggcacgg aactgccggt actgtacgcc ggccttacgc cgggttgtgc
    38941 cggcgtctat cagatcaacg tccgtgtgcc gcattgggcc ccggcaggca gcaaggttcc
    39001 gctgacgatc gatcaagggg actcttcgac caccgttacc gtccgcgtcg tgcaataagt
    39061 ccaatcgaag ccacgggaga tctcctcccg tggctcggac ctgccgatct aaccctcgga
    39121 agcttatgcg gattttgatg ccagttgtcc tgctcacggc cgccgcggca ggccttgccc
    39181 agacatggga ggccggcggt tccgccggcg gaagcttgtc gaggaatgtc ggcgtgtcca
    39241 gccaattggg tcaggccagc gccggtcttg ctagcggcgc cgccttcggc gcagtcctcg
    39301 gacagagcat ccaccgcatc gtcagcggcg agattcgcta caccttccaa ccgggcgaaa
    39361 tccaggttgc ctccggcggc acacggacca gcttccgcag ccaattccac gcgattcact
    39421 acgacctcct gttccacacg cgtccggaag gttcctccgt ccggcccttc ttcgcagccg
    39481 gcggaggcgg acggatcttt aagggcacgg gaaaggagaa cgcctaccaa ccgcttcagg
    39541 agtacgctct gctcacaagg acgcgggagt ggaagccgat ggccagcgtt gggggaggcg
    39601 tcaaaatcgc aatctctcca cgaatgttcc tgcgtgtcga gttccgcgat tacatcagcc
    39661 ccttccccaa acaggtgatc gctcccgccg atggttcgaa gatcagcggc tggctccact
    39721 ccgtcgtccc actagtggga atcagcgtgc ccctttaaaa cagggcggcc agcccattaa
    39781 atccgccaac caccagaatt agcaactgtt tcgacgcttt tcgccttata gtgggggagg
    39841 tgcgagcttc gttcttctgg gtcatttcct cactcgctgt cccgctcacg ctcgcgggac
    39901 agaacgctcc ggcgaatcag tacgttggcg gcgaagtttg ccgaaagtgt catccgaacg
    39961 tctggctcaa tttctacaag aacccgcatt acaagagcgt cgcgagcggc actgaggctc
    40021 cggataagac cggatgcgag ggttgccacg gcccggggca ggcgcatgtg caggcgtccg
    40081 gcggcaaagc caccatccgc gccttctccg tcatggaacc gaaacaggtg attgacgcct
    40141 gcctcgcctg ccatagcagg gatctctcga aggcgaacat ccgccgttcg tcgcacaccc
    40201 tggcaggcgt cgcgtgttca gactgccatt ccattcaccg ctcgccgatc ccgaaattcc
    40261 tgcttgcgaa agagcagcgg gacgtctgct acggctgcca cgcgccggtt cgcagccagt
    40321 tctcgatgcc tttcaagcac cgtgtgaacg aaggcttcat gcagtgcacg gactgccata
    40381 atccacacgg cgcacccgcg gcgacgtggc gcatgggagc ccgtccgcga atggttgagc
    40441 atgcgctcgc caatgaggag ccgtgcctga agtgccactc ggacaaacgc ggcccgttcg
    40501 tcttcgagca tccgtcagtg cgtgtcgacg gctgtgaaac ctgccacaat ccccacggct
    40561 cgacgaacgc caagcttctg cggcggccgg tcgtattcac ggtctgcctc gaatgccaca
    40621 acggcgcggg tgcctttggc cggcagggcg acgggatcag gacgcagtct gcctcacaca
    40681 acatggccga cccacgctac cagaactgca ctgcgtgtca cgtgcgaatc cacggctcga
    40741 acgccgaccc tctctttctg cgatgaggtc acttctgatg aggcgcaccg tgttcttaat
    40801 cctcgcttcc ctcgggccgc tggcgggcca gcagaccgtc actcctacgg cagccacggt
    40861 cgggcctcct cgcggaacgg agatcggcaa ctacaatgtg atgaactcct tcgagacggg
    40921 ataccggttc cgctccgtgg gtggcgatct cggcaagtat cgaagcgacg tcaacttcgg
    40981 caacggcatc cggcttctcg gaagcaggct caccgtcaac tcgaaggacg gtcacggcag
    41041 cctcttcgac gagattgtgc tgtccactca gggtctcgga aacgatccat accagttctc
    41101 gtcgctgagg attcagaaga acggcctcta ccgttacgat ctcctctggc ggctcagcga
    41161 ttacttcaac ccagcgcttc cgcttgccgg aggcctgcac cgcatggaca ccgagcgccg
    41221 gatgcaggat cacgatctgg cgctacttcc gcagtccccc gtgcggttgc tgctcggcta
    41281 cacgcggaat tcccaagacg gcgcggccct cacgaccgtg cagtccttca gcgctggcga
    41341 cgtctcaccg gtgtttgccg acatccgccg catccggaat gagtaccggc tcggaggcga
    41401 agccgaactg ttcggcatga agctgcacgt cgtgcgcggc tgggacgact tcaaggagga
    41461 cacgcccttt acgcttgcgc ccggtaccgc tggcacatcc tccccattca ctaccttccg
    41521 gcggaacgag ccgtatcacg ggacaacgcc ttggtggcgc gttcatcttc gcggagaaaa
    41581 caagtactgg gctgcggcgg gacatttcac gtacgcaatt ggccggcgcg attttatctt
    41641 cgatgaggcc gccgtcggaa ccgaccggtt cggctccgag cgtaaccggc aggtccttgt
    41701 cgccggcgac gggcggcgcc cggtcacctc tggaaacgtc actctcagct tttttccgac
    41761 ggacaaccta accatctcca accacaccgc cttccacaac acgcgcatgg aaggcgattc
    41821 cgtgtacaga gagttaaaca acgccacggc ctccggcccg tatctgttct tcaacttcct
    41881 cggaatccgg tccataacaa acacgaccga cgcgaccctg cgcgtctcga aatggctggg
    41941 gctttacggc ggctattact actccacccg gcgcatccgt tccgtcgagc aggtggagat
    42001 tgatggtgtt ccacagcggc tgcctgcgag tcaggacaac cgcctccaat ctgggctcgc
    42061 gggaattcgt ctgaacttcg cccgctccct gacggtaagc ctcgacgccg aggtaggacg
    42121 cgccgaccgg ccgttcttcc cgatttccga gcgtgactat cacggcttca acgcccgcgt
    42181 gcggtacaag tcaaagtcgt tcgccctctc ggcgtccgca cggacgaact acaacacgaa
    42241 ctctgtttct ctgtcgagcc acagctcccg aggccgtaat tattcggtgg acggctcgtg
    42301 gacaccacgt gactggttct ctgtagacgg cggttattcg aaacttcacc tcgatacggc
    42361 ttcagggatc gcctacttcg tcgattttga gttgattcag ggcgagcggt cgatctatgt
    42421 cagcaacatt catgcagcca acctcggcgt ccgtttcggt ctcggaaaac gcgctgacgt
    42481 ctacaccggc tacagtcatg ttgaagacac gggtgacggc cgcgtcaatc ctttcatcgg
    42541 gtcgggcgcg cgacccgatc ccttcgtgcc cgcacagacc ttcccacttg cgttccggag
    42601 cccgctcgcc cgcttatcgg tccgccttca cggcaacctg cgctggaatg ccggctacca
    42661 gcactacaac tatcgcgagg acttcttcgg ccggcagagc taccgcgccc acaccggcta
    42721 tacgagcgtg ctatggtctt tctaaaccat ggagctcaag ctcacgctct tcaacagcat
    42781 cacgctgatg atcatgggct ttacgctgct gacggtgatc ccgctcgcgc gaggacgcct
    42841 gaaccgtaac tggccgtttc tttactttcc tttggttttc gcctactggc gagccttcga
    42901 cggcgccctg aatacctggt ggatctgcgc cggactggtc attgccctgc tcctgcgctt
    42961 cgagatcatc ggtgcaagat cccgtgcgcc gttgcggtgg ctgtcagtca tcgcactggc
    43021 ttatgttttt ctgcggggag tggatctgct ggtgggaggg gaactgttct acttcttttc
    43081 cgtcggcagg ccggacgcct gatacgcccg ccagcctccg tcgagcgaga taacgtccgt
    43141 gtagcccatc ttctggagcg cgtctgccgc gagcgcggag cgatacccac cgccgcaata
    43201 aagcaccatt tttgtgctct tttccgggac atgagtctcg atatcgcgct cgataattcc
    43261 cttgccgaga tgcagcgcgc cggcggcatg cccggcagcc cactcgctct cctcgcgggt
    43321 gtcaacgagg aggaacttct cgcccgagga gaccagcctc ttgtagccgt cgatgtcgat
    43381 ctgccgcacc cgcgacttgg agtcgttgac cagcgcgaga aagcctgggt tgtgccgttt
    43441 gccgccgtga gagagagcca taagagtcat gcggcgcgct ggatgcgggc gcctaccttt
    43501 cccagtttct gttctatttt ttcatagccg cggtcgatgt ggtacacgcg gtcgatcacc
    43561 gtttcaccgc gtgccaccag ggccgccagt accagagacg cgctggcccg caagtccgac
    43621 gcgatgacgc ccgcgcccgt cagttcccgc ggaccagcga cgatcgcctg ccggccctcc
    43681 gtgcgaatgc tcgcgcccat acgcaccagt tcgagcacgt gcatgaagcg gttctcgaag
    43741 atggtctccg tgatgaacga gattccctcg gcctgcgtca tcagcgccat gtattgcgcc
    43801 tggaggtcgg ttgcgaaccc cggatgttct tcggtcgtgg cgtccaccgc agtcagacgg
    43861 ccggtacggc gaactctaag cgacgactca ctttcctgcg aaacatccac tcccgcctgc
    43921 cgcatcttca caatcagcgc gccaaggtgc tccggcacac aacccttcac gagcagttca
    43981 ccgccagtga tcgcaccggc gatcaggaac gtgcccgctt cgatgcggtc cgggataatc
    44041 gtgtgttcgg cgccatgcag ccgtttcacg ccgcggatcc ggatcgtgga ggtaccggca
    44101 ccttcgatgc gcgcgcccat cctcatcaga agttcggcga ggtcggcaac ctccggctcg
    44161 cgcgcggcgt tctcgagcac cgtctcaccc tcggccagca ccgctgccat catcaggtcc
    44221 tcggttcccg tgacagtgat ccggtcgaag tgaatgaacc ctccctttaa accatttggc
    44281 gcttccgctt ccacgtagcc gtgctcctgg cggatccgtg ctccgaggtg ctccaggccg
    44341 gccaggtgca ggtctatcgg tcgcgccccg atcgcgcaac ctcccggcaa cgacacgcgc
    44401 gcccggccgc aacgcgcagc cagcggcccg aggacaagac tcgatgcccg catcgtcttg
    44461 acgaggtcgt acggcgcctc cggggagacg atctccgccg cccgcacacg aaccgcgccg
    44521 ccgttcgcct tggtggaagc gccacagcgc tcgatcagct tctgcattgt gcggatgtcg
    44581 cgcacgtggg gaatccggtg cagaaccacg ggttcgccgg tcagcagaca cgcggccagc
    44641 gccggcagcg ccgagttctt cgcgccactg acttgaatct caccggctag aggtacgcct
    44701 ccctgaatcg cgaacttatc cacgccgcaa cgcctccgac acccggccgc cgcaggccgt
    44761 caacttcttt tcggcttcct cgcgggagac cccgagcctc gccatcacaa tcgcagttct
    44821 gacggtgccg ccggactgtt cgagcaacgc tccggcctgc tcgtaatcga cttccgctgc
    44881 ctttgcaatg attctcctcg cacgattgac gagtttctcg ttggtcggct gcacgttgac
    44941 catgaggttg ccgaacacgt acccaaggcg aatcatcgtc gcagtcgaga tcatgttcaa
    45001 gacaagcttg gtcgccgtgc cagccttcag ccgcgtcgaa ccggtcacga cctctggtcc
    45061 cggaagcggc gcgatcggaa tttccactgc ccgcgaaagc tccgagtccg gcgtgcaact
    45121 gatccctgct gtcacggcgc cgattctccg cgcggcggcg accgcaccca gcacatatgg
    45181 ggtgcggcca ctggccgcga tccccacaag cacgtctttc gcgctgaagc cgcgatcctt
    45241 aaggtcgcgc gcgcctcgct ccggatcgtc ctcaatcccc tcggccgaat gggcgagcgc
    45301 ccgctcgccg ccggcgatga tgccctgcac cgttcccggc gatacgttga acgtcggtgg
    45361 gcattcggag gcgtccagca caccgagccg tccgctcgtg cctgcgccga tgtagaacaa
    45421 ccgcccgccc tgcttgatcg ccgctacggc cgcatccacc aggcgggcga cgttaggaat
    45481 ttgggaagtc accgaggcgg cgaccttcat atcctcggcg ttgaagatct gaagtattcc
    45541 ttcggtggag agggcgtcaa ttccttcgga ttcgggattc ggctgttcgg tcaggagttt
    45601 accgagcatg gccctacact gcgcctgcct tcttcatcac cacaagtgtg gtctgcagac
    45661 cgaggcgcgc cccgtcctcg ggggccgtgt ccttcttcag tcggccaagc acttctggac
    45721 tcacgccgta cttgtaaccc accttcttca gagcttggaa gaagccggcc aggtcgatga
    45781 caccttctcc cggcatcagc cgctggttgt cgcgtatctg atccgggggc agcttcggcg
    45841 cgtcggacac atgcacgtgt acgatccgct cggcccctgc gtcctcgatg tcgcgggtcg
    45901 tagcgcccgc atgatgccaa tgccacgcat cgagcagcaa ccccacggtc ggtccgcact
    45961 ccttgcagaa atcgagcatc tcgtccattc gccagataaa ctcgtgcgga tgcagtttcc
    46021 ggagatgcat cgggctcacg aactcaaggc ccaggcgcat tcgcgagggc gccaggatcg
    46081 ctgcgaccgc ctgaaatcgg tctttgtaaa tcttgcgcag ctccgccttc ggagtgtcgc
    46141 tcgaaggcag aatccaagtc agcagccccg gcgctccgat ggaggctgag aacttcactg
    46201 tcggctcgag cttcttgagg tccgcctgga acgttgcttc gtcttttcgg aactcaacca
    46261 gcaggtcggt cgccgcgggc ttgagtttga gcgtcttcag aagtgcgcgg gtcgaatcca
    46321 gtccctcact catcgcccgg ctgagataga tttccgttcc gggataaccc agccgggcgg
    46381 ccatgtgagc gaactccggc cagcccacct tcgcacctgc gagcagcgaa ccgttcaaac
    46441 agacgtacac cgcatcctcc taatagttct tttctttctc tacccgcggt cggtcggcac
    46501 ggctcatcga cttgacgcct gccagggaca tgtccgcttt caccaacgca tccgtacccg
    46561 ccatcacctt cgcgaccgct ctcccgatgt cctcgacatc ggcctcgtcg ccaagcagca
    46621 ggaattgcgg aagccacacc gattcctcgt aggccgcccg ctccgctacc gggcagcgga
    46681 aggcacggta gttgactggc tcctcacggt cgatccgaag ctgcgggaag tcctccggct
    46741 tcgcaccgaa gaggtcgctg cgatagaccg gctcatagaa ccgtccgtcg ctcggaatgc
    46801 cctcagcatc cagcgccgcg acgaacaggt cgcgagagac gcgccgatcc gccggccgat
    46861 attggaaatc gtagttgtag atggcctcgc gcgtcagcgc gggctgggca ggcaacggac
    46921 ggacaccgtc gattccagcc agcattttgg tcagcagcgt ggcgtgcctc gaccgctttg
    46981 cggcaagctc ttccagccgc tccagttgtc caagcaatac cgctgcctgg aactccgtga
    47041 tccggtagtt cgatccgacg atccgcttct tgaactggtc cgtgacgctc gcccggccgc
    47101 agttgaccag cgactgcacc agctcgaagc aatccagccg tgaggtaatg acaatcccgc
    47161 cctcgcccga ggtcatcagc ttgctggact ggaggctgaa acagccgatg tcgcccattg
    47221 acccagcgcc cctgcccttg tactgtccgc cgtgcacgtg ggcgcaatcc tcgatcacga
    47281 tcagatcgcg ctcctcggcg agcttcagca gcgggtccat ttcggcaaat cgcatggcga
    47341 ggtgtactgg cagtagcgct tttgtgcgtg gcgtcagcgc ttgccgggcc gcctcgacgt
    47401 ccatgcaata cgtatcggga tcgacgtcca cgaagatcgg gatcgcgccg gcgtctagca
    47461 ccgccgtggc agtaccgtcc caggtgtagg ccgggacgat cacttcgtcg ccgaagccga
    47521 cgcctgcagc cttcagcgct gccagtatgg caatcgtccc gttggcaagg cagcagccgt
    47581 accgcgcccc atgatgggag gcgaatttcc gcgcaaactc atccgcaagc aggtttggaa
    47641 acgggtaacc accccagttg cggctctcga gcacagctac caaccgctct cggtcgcggc
    47701 cgtcgtattg gggccatgcc ggaaagggct tgtttcgaac gggcttgccg ccgaggatcg
    47761 ccaagtcgct catctgaact agtatcctat agggaacttc ccggcgatac cgctttcggc
    47821 ttatggaccg ggcggtctca aggagagcga tgggcactcc cgtggcgttg ggacggcagg
    47881 tctgtggaag tgcgttgata cccccgggcg cggcgtgaac cggcgcccgg ggcggaagga
    47941 atcccggatg ggcctcaagc gttgaatgaa ctgccgcagc cgcatgtcga cttcacgttc
    48001 gggttatcga acttaaaccc tgacccttca agggtttcca cgtagtcgac tttggcgcca
    48061 tcaagataga gcatgctcgc ttggtcaacg aagactttga ggccgtcaaa ttcgtaagtt
    48121 ttgtcgagga ggcccgggct gttctcgaaa gccatggagt agctgaaacc ggagcaaccc
    48181 ccacccacta ctgcgattcg aagaccagcc ggcttgggct cctgcatgtc caggatttcc
    48241 ttcaccttgc cgacggccgt ctcggtaatc tgaatcatat gtaatctccc tctatcagga
    48301 ctcctacccc tcccattcta caggttgagg cggtagcccg ccaatctacc ccgtcactgc
    48361 cactttatat cgatgtggct cccaggcgca ggtttcgcga atctggtatg ttgttcgagg
    48421 agccgtcggg ggagaaacgg cgccgggtct tcggaaaggg agatcgaggc gccgcaagac
    48481 gctgaaaccg ctcggttgtc tgccgcgtcg taacaactgt atgagcggga tcagcggtgc
    48541 actcccatta ccgttggcgg gcggacgaga gcgcgcaaag ggcaaggtgg actaccgcaa
    48601 taccgaggag ggccttcttg tacgccgggt gcaaaaaggc gacgagatgg ccttccggga
    48661 cgtcgtggaa cgataccaat cgaaggtgtt ctccatcatc tacggtattt tgcggaatca
    48721 caacgatgcg gaggacatcg cccagcaggt gttctccaaa gtctatttct cgatccggaa
    48781 tttcgatttc cgcagttccc tgctcacatg gatttacaag atcacagtga acgagtgtta
    48841 cgactatctt cggaagaaga gggtccggaa gctcgtttac gagagcgatt tcagtgaaga
    48901 ggacaccgct cgcctgcata gctctgcggc ggtgaaagac gcaagtccct cgtttgatac
    48961 aagccttgcg cagcgcgact acgtgtggaa actgctcgag aaagtctccg aagaagaccg
    49021 caggcttatg cttttgaaag aggtcgaggg acattcggtt gaagagttgg ctgcgatgac
    49081 gggcatgaat gagaatacaa tcaaggtgaa gctctttcgt gcccgtcaga agctgctgaa
    49141 ggttgcccag cgactggccc gttccacggg cacggcgacg gcatgacttg aggaacgcgg
    49201 acggaagtgt ataatccgca caatatggtg gaaacgctct ccggcagaac ggacaaaaga
    49261 tccgaaatgg agaccgggga ggcggatttc gcaggctttg agggaagaag tgatgcatta
    49321 tcccattcgg gaaaagcttg aggaatacct gcgaggcgaa gctggtcccg gcgagcttcg
    49381 ggagttccac tcgcacatgg aaacgtgcag ggagtgccgt gacagtgtga ccggaatgcg
    49441 ggatcaggct cggctgttgc aatgcctgcg ggcgtccgat gcggtggagc cactccccgg
    49501 tttctacgcg cgggtgatgg ggcgcatcga gtcgcagcgc gccaattcaa tatggagctt
    49561 tctcctggag cccgttttca gccggagcct ggttgtagca tccgtgcttc tggtgcttct
    49621 gcttggaggc tatgtcgctt cgggtgagtt tgacaggccg ctcaaccctt ccagccctga
    49681 agtcattctg gctgccgaac cgaatccctc ggagatggtt ggcctcaacc cgcaacgcga
    49741 ccgcaacacg gtcctgctga cgctggccac ataccgggat taaggtcaaa tgtcctggga
    49801 aaactgctca tcctggcgga atccacgggt tctcctgagt ttgactctga tcttcctctg
    49861 tggtgtcgtc ggcggcgcca tggtcatgcg gtttgcggtt cacggcatcg ttgataaggc
    49921 tgagacgggc ttgccgggtg gcgaaaaggc gatcgcgctg gagcgcatga ccaaagaact
    49981 cgacctcagt gccgaccagc ggcgggaact cgagacggtc cttgacgatt tcgtcatgta
    50041 cgtgcaaaca ctccaggcgc agatggacga agttcgcgcc aacggaaaac aacggatcct
    50101 ccgtatcctg aacgaccaac agaagcagaa gttcgaaaag atcctggtcg acgtacaggc
    50161 gcggcagacc gcgcgggttc cttgacggtt cgcgttgatt cgtagggctt cctttacttc
    50221 cgttaggtct acaatacggg tgaggccatg tccgtggcgg ccacccacga ttcgccgtac
    50281 agtggttcct ggtacccggc tgaccggatc gaactcgatg agctgattga ccgcttgtgg
    50341 aagtcgtccg ggcagcgaac gggtgcatac ctgctcgaaa atcctctcgg attcgtcgtg
    50401 ccgcacgcgg gcctcgtgta ctccggaacg gtagcggcgt ccgcctaccg tcacatcgaa
    50461 agatgccgcc ccaagcgtat cgtgctactc gggtttgccc atcgcggctg tccaccgggg
    50521 ctgtggattc ccgatgtcac ctctgtccgg acgccgcttg gagaagtccg gatcgacgcg
    50581 gaggccgtcg cagacctcac ggtcgggggt gaattccaga ttctgcctga ggcacggctc
    50641 tgcgaccatt ccatcgaaat ccagttgccg ctcatccaaa gggcggcgcc agactcgagt
    50701 gttgtgcctg tctatgtcgg ccagttgagc gaccaagctc gccgcgaaga ggcgagcgcc
    50761 ctcgcgcggc ggatccgcgc gggcgacctg ctgatagcca gctccgacct gacccattac
    50821 ggcagcgcat tccgatacca acccttcccg gtggacaacc gtgtgcagga acgcctgcgg
    50881 gtcctggatt tcggcgtatt ggatgcggcg gcaagccttc gaccggaact gtttctcaag
    50941 accttgctcg aatcatccgc caccgtctgc ggctatgagc cggtttccct gctgctgtcc
    51001 gcgatgcggg cgagcgaaga gcagggagag gtcttccagc aggtcatcga ctaccaaacc
    51061 tcgggggaga tcaccggcga ctttcaccac tcggtgagct atggtgcggc cggttatttt
    51121 ccgtattcat cgttcgaggt aaactccgag gcgcgggaag tgctgctcga tctcgccagg
    51181 cggacgcttc ggcagtacca gacaaccggc cggcggcccg tttccgtggc acccgggcaa
    51241 gatcacgccg ttcttgagaa gaggtcggca gcgttcgtga cgcttcaccg gggcggtcag
    51301 ttgcgcgggt gtatcgggcg caacacgagc gacgcgcccc tcagagaggt gattccggag
    51361 ctgacgatgg cagcggcact ggacgacacg cgtttcggac gcgtcacccc ggacgagact
    51421 ggcatcgaca tcgagatctc actactgaca ccaatgaagc gaatcgtgga ccgctctgac
    51481 ttccgggtaa acgcgcacgg tgtgttactg caatccggtt accggcacgg tctgctgctt
    51541 cctcaggtgg ccaccgaacg gaattggggc gccgaacagt tctttctcgc actggcacgc
    51601 aaagcgggat ttgacccggg tgtgtacgac gatccagcca ccaagctgca tgttttccgc
    51661 gcgcagatca tccactaatt cggcctcccg tatactgagc tggtgatgct ccttctgacc
    51721 ggtcctaccg gctctggaaa gacgcgccat gtgcttgagc gtgtccggga ggcgctcgcc
    51781 tcccggcgca cggattttcg tctgctcgtg cccaccgcga cgatggcgga gcacatccga
    51841 aacgagcttg cgcgggaggg ctttgtaccg cgccgctcgt tgatcgggac tctttccgga
    51901 tttgtcgagc aattggccgg ggacacccca caagtaactg gagggcagct caacctgatt
    51961 gtggaggagg ttctggcgtc gatttccccg aacgagttcc gcgaggttgc ttcgctccca
    52021 ggtttccgca cggccctcgc tggtctgatc gacgaactga cctcagccgg gtgtgactcc
    52081 gtgcaagttc gtgctgccgt cgtccgacgg ctgccaggcc ggactttggc ggccgctttt
    52141 ggcgacgtct tcgaggaagc ggagcggagc tggcggcggc gtggggcggc tgcccggggc
    52201 gagcgtctgc ggttcgcggc gagccgcatc cgggagcaag ggctacccgg cgtccgcgcc
    52261 gtctacatgg atggtttttt cacactgtcc cagccggaga tcgaattggt cgaggctctc
    52321 cgcgcgcacg ccgatctcac cgtgacactg ccgcgttggg agggttcaca acaggctcgg
    52381 gcccggctgc tcgggatggg cttcgtcgag gaggagcaca cccgggcccg acagcagccc
    52441 cgcattgttc tgcttgctgc ccggaccacg gaggaggagc tcggagagat cgcgcggcgc
    52501 attctggccg aggcatcggc cggccggccg tttcgggaaa tgggcgtcgt tgtgaggagc
    52561 cgcgagccct atgtacctgc gctccgtgcc gttctcgaac ggttcggaat cccggcgcgt
    52621 ttctacttcg ccgagccgcc ggtggcctac tcgccggtgc gttatctggc gggcgcggtg
    52681 gacgcgatgc tgggtggttg ggaccacgaa gagacgctcg gggtgctccg gatgacctcc
    52741 tccgccgcgg gtcgctgttc ggacatggac cggtttgact tcgaggtgcg gaaaaacctg
    52801 cccggacgcg ggctggcgcc gctccggctt cttgctcggc acccagcgat tcgcaccctg
    52861 atcgactccc tggcgccgct cgatgcatgg cgggaaggtg aatgccagcc gggggagtgg
    52921 gccactcggg taaagaatct cagttcccgg ctgttccgcc cggtggcccc ttcggactca
    52981 ccgtcacgat ccctcattac agtctggcgc gggcaggcag ccgcgcttcg agcgtgggag
    53041 ggagccgcta acgaggccgc cgccgctctc gacggcaaag gccccgtcgc attcgagccc
    53101 ttctggcacg cggtgaatga agcgctgagc gagacgaacg tgcgcgtacc cgaccagcgg
    53161 cgcaacgtcg tacacgtcct ggacgtttat gaggcacggc agtgggagct tccggtggtc
    53221 ttcgtctgcg gtctcctcga gaggcaattc ccgctgtatc accctcagga ccccgtcctc
    53281 ccggataatg tgcgctccgt cttgcgacaa gaaggagtga cgcttcgaac agccgccgag
    53341 cgggatcgcg aggagcgttt tctctttgac ctggcggtct ctcgcgcgac atccacgctg
    53401 gtgctgagtt acccgcaatt caacgagagc ggagacgcca atctcaggtc cttctttctc
    53461 gacgagatcc aggctacggc ggttcaggaa cagagccgcc cggtacggct cgccggcggc
    53521 cccaccagcc gcgtggaccg gccggttgtg atccgcgacc ccggcctcct cgaccggctg
    53581 gtcgagaagc acgcatcgac aagctcgtcc gccgtcgagg ggtttctcca gtgcccattc
    53641 ctcttttttg cgcggaacac tctgggcctc gagggcccgc ctcgccaccc ctctgaacgg
    53701 tttgaccaac tcgcgcaagg agacgtcatc cactgtgttc tcgcgctgtg cattgggtcc
    53761 ggtgtgcctc tggaggaagc gttcgacgcc gagttgcggt gggtctacaa ggaaagaggt
    53821 attcccgacg gttaccggcg cgaagccatc cgactggagc tgtttcgcga tttgcgccgg
    53881 ttcctgacgg atctcgaact accgcctgca agcaaacgcc tgtacgagca ctccttccag
    53941 ttcacgcttc gtgaaggcgt tcggctgcgg gggaggatgg accgcgttga cgtctacgag
    54001 gatggcctcg ccctggttgt cgattacaag tacagccatg cccaaaaggt gcgtgaccgc
    54061 cgcaaaggaa cggaacaggg tcgattcgta caggcggggt tgtatctgtt ggctgtcaag
    54121 caggtgctcg gccacgaacc ggccggaatg ctatttgccg gactgcggcg ggagacgagt
    54181 cttgaaggct ggcatcttcc tcttccaggc ctcgaggccg gcgcagattg tacgtcggag
    54241 gtactggccg aactgtcggc gactgcgtcc gggcaggtcg ccggagtgct ggacgccgta
    54301 cacggtgggc ggatcgcccc atctcctgcc gacgaacgcc tctgcgagta ctgcgagttc
    54361 gtcgacatct gcagggtaga gagcgccacg ggcgcgacgg ccgcgggagg gttcgaggaa
    54421 tgacactctc gccaaagcag aaggcggccg tcgaacgcag tggacaggat gtctgcgtcg
    54481 tcgcgggacc tggctccggc aagacccgtg tgctggtgga gcggttttgc tggctggtgt
    54541 ccgcgcgtgg cgtatcaccg ctccgcgttc ttgccataac gttcaccgaa aaggccgcaa
    54601 cagagatcaa gcagcgcctg gtcaacgcct tctccggcga tgcgaaagtc cgtgagcaga
    54661 tcgagcgcac gtacgtttca accatccatg gtttttgcgc acggctgctc cgcgagaact
    54721 cggtggccgc gggactcgat cccagattca tagtgatgga cgcaccggcg agcgcggccg
    54781 aactcgacca tgcggccgat gcggcactcg aacggctcta caccgagcgg ccgtccgagt
    54841 ttcgcgggct gctggacgcg agctacttct cgacagacct cgcaagttgc ctgatccacg
    54901 tctatcatgc gcaacgagtt gccggcgtcc cgctcgcgcg cggaagggat gcatcgcccc
    54961 ctgcagagag cctctccggc attgtccagg agttgtacga cctgctctcg agggttccgc
    55021 cgccaaagac aacaccacag ataggcagaa ttacctcgat caagggctgg ttggagcgcg
    55081 ccggaacgtt ggaacggaaa cccttgggtg cggaacactt ccgcctgctt aaagatttca
    55141 agtgcgacct taacacgttc aagccaggtc ccgatcgcga agccgcccgc gatttacgga
    55201 agagacggat tccccgactt caagcattgc tggcaacgga atactacgag cggttccgcg
    55261 cgttgctggt ggaggcaatc gagcggatcg acgaggccta caggtcgcga aagcgggaac
    55321 tgagcgcgct cgatttttcc gatctggagg agcacacgat caggctgctc gaaggaaatc
    55381 ccgaggtgcg cgattccctc cggaagagct tcgactacat tctgatggac gagcttcagg
    55441 acacgaatcc gctccaatgg aagatcgtga acctgatccg gtcaccggaa cggttctttg
    55501 ctgtcggtga catcaaccag tccatttacg gattccggca agccgcgccg gaagcgttcc
    55561 gggagtaccg gaaaggcatc gagagcgcaa gatggaaagt cgacgatctc gacgagaatt
    55621 accgcagccg tcaggcgatc ctttcggcgg cggaatgcgt cctggcgggc gctccgggta
    55681 tcgagctgcg cgaattgaag gcgaagcggc aattctgccg gaaaaccgaa ccgtcggtgg
    55741 aggtgatcgt agcttgtgcc gcgaaaccgg cggatccggc ggacgtcgag gcccgctgga
    55801 tcgcacggcg catccgcgag ctcgaaggga aactgaaaat cggatctctc accgatcccc
    55861 gcgcggcccg ttttcgcgat tttgcgattc ttgtgcgcgc gaacaccgcc ctcccgccga
    55921 ttgagcgcgc ctttaaggaa tttgatgttc cctatctgac gacaggtggg caaacctttt
    55981 atgaggcccg cgaagtgcgg gatctggttc tgcttctccg cacgatcgac aatccacggg
    56041 acgagatcgc gctggcaggc gttctgcgat ctcccctggg aggtgtcgcc gacgagacac
    56101 tgttgcggat gaaatcgcac gacaggctcg gcactgtgct gcggcggctc gatcatctgg
    56161 acctttcctc gttcgacgcc ggggacctcg agcggctccg gggattccgc gatctgttga
    56221 accggcttcg gagcgtacgc gacgacgttc cgcccgatcg cctgctgctt gacgccatcg
    56281 acggctcggg atacggaagt caactggatg ggcgcgcccg ggccaatgtg gataagctgc
    56341 tcgcgctggt ttgtgactgg cactgtgcgc agccgcgtcc ggtgaacgaa ctcgtcgagg
    56401 atctcgaatg gattcgcgag gcggcaacag aggccgacgc gccgccggac gactcctcga
    56461 atgccgtgcg ggtgatgtcc atccatcagg cgaaggggct ggagttcccg gttgtcttgc
    56521 tcgcggcgct ccatcgccat acgaacgaca cccagccttc actgtgcctt gcgccggaag
    56581 gccgactcgg agtccggtgg cggaatccgg cgggaagcgg ctatgtggac gacgtcttcc
    56641 atgtggagtc catggccctg accgacgccc gtgagcgtga ggaggaggac cgcctgctgt
    56701 acgtcgccat gactcgggcg gaggagcacc ttgtactctc gttcgcgagg actgaccggc
    56761 ccggcgcgag ggcggagagg gtcgctggag cgttcggcat cgatctccaa cagaccgacg
    56821 cagggccgcg ggtttttgcc cccgtaggcg cccatttcga ggtgcggttg tttcgcgcag
    56881 ccggcccacc tgaggcggtg gaaatgcgtg agggagactt ctcgttgccg gtggaagaac
    56941 tcctcgtacc tcatggcgcg gcgtccagcc agcacgattc ctccgcttcg gtgacggcga
    57001 ttcttgcctt cgaacaatgc ccacgccgct attttctcga ccgctaccta ggatggagcg
    57061 gcgtctccca gccggccgcc gctgaagaat acgagagtca gtccgcacgc gaggagttgg
    57121 acgaacccga cgcagccgag ttcgggcttc aggtgcacgc tctgcttgcg ggcgccgcca
    57181 tagagtctcc ggacgcggag gctgtggaac tgaaggctcg tttcgatgcg agcgcccttg
    57241 gacgccgggt ccgtcgcgcg gacaggatcg aacgggagtt cgatttcctc cttgccgtag
    57301 acgacatcgt gctgcacggc cagatcgacc tctggttcga ggaaaacggc cggcttgtgc
    57361 tcgtggacta caaaaccaac gacattcggg atgaggatgc ccactctcgg gccgatgact
    57421 actcgttgca actgcgcctc tacgcgcttg ccctcgaacg ggtgtgcggg cgactgcctg
    57481 acgaagcgta catctgcttc ctcaggccgg gcaggtcagt ctctgtggat ctctccgaag
    57541 cggccctcgc ctctgcacgc ggaagcgtgg ggaggtttcg cgaggcgcaa aacaaactca
    57601 gtttcccgtt acgcgaaagc ccgcagtgcc gagcgtgtcc gttctaccgc ggtttatgtc
    57661 ccgccggacg cggacaggct gctaaggatg cggacccgcc atcttctttt caagagcgtt
    57721 catgaggcgg cggtgcagat cctcagccag gggctgaggg tccatgccct tgtaaatctg
    57781 gatggtccgc ttggtggtat ccaccatcac ccagcagttc gggcactccg taacgtgcgc
    57841 ctcgagttgc tggcgcatct cctcctggat ggtaccgtcg aggtactcgg agagttcgtc
    57901 catgaaggcc ttacaagtaa gcaaagacgt cgtctccttt ccgcttgaaa aatcgcgtga
    57961 gccggtccct aagctgcaac cgcgcgcgca gaagccggct cttgacggcg ggaatggata
    58021 tgttcagcac gccggccgtc tcttccgtgg aaagctcatc gatatcgcgg agcacgaaca
    58081 cggtacgaaa gatcggcggc agggcgtcga tcgcctcgtc gagaatcctg cgcaactcct
    58141 ctctcgaata ccgttcctcg ggattgtctt cccagaccgc gatctctcgc gctacagtct
    58201 cttctccagt gtcgatgccc tcgtccagag acaccgtccg tgccgtcctg cgcttgcgga
    58261 gcttcatcag cgactcgttt accgcaattc gcacaagcca cgtgtagaac tttgactgcc
    58321 gctggaactc gggcaggtgt ccgtaagcct tcaggaacgt ctcctgaaga acgtcttccg
    58381 cgtcttcctg gctctgtgtg atgtgcttcg caagcctgaa aatcttacgc tcgtactggt
    58441 tgacgagttc tgtgaaggca cgcgcatcac cgccctgggc ccgttctacc aggttcgatt
    58501 cgtcaaacgt aatggcttgc gtggaaggct ggcccatcac ggacccgtgt tctcctcctt
    58561 caatcttagc gcggaagccg gccgcggcgc tgccgcagcg cgacggacga aagtggagcc
    58621 tgtgttacgc tgaaaattca gcccttatga gcaccatcag tgtcactctg ccggatggca
    58681 gccggcaaga gcatccggcg ggtgtccgtc ccatcgatat cgccagaaag atcggatcgc
    58741 gcctcgccga cgaggccgtc gtcgcgcgcg ttaatgggag cctgtacgac ctgacgcgcc
    58801 cccttgaatc ggatgtaaca ctcgaaatcc tgacgacgaa gaacccggaa gccctcgaag
    58861 tctaccgcca ctccaccgct catttgatgg ctgccgccgt gctcgaactg tatccaagta
    58921 cgaagctcgg gatcgggccg ccgatcgaca acggtttcta ctacgacttc ctgcgcaacg
    58981 agccgttcac gccggaagac ctcgagaaaa tcgaggccaa gatgtgggag cttcagggga
    59041 aggacctgcc ctacgagcgc aagctcacgc ccaaggcaga aggcctgaag aagtacgccg
    59101 aaatgggcga gcagatgaag tgcgagctga tcgaggaacg cgccgacgac atgttctccg
    59161 aatacaccct gggcccgcat ttcatcgact tctgccgcgg cccgcacctt ccttccacct
    59221 cgcggatcaa ggcgttcaag ctgctctccg tcgccggcgc gtactggcgt ggcgacgagc
    59281 atagccacca gatgcagcgc atctacggga cggctttctt tacccggaag gaactcgagg
    59341 cctacctgcg tcagcttgag gaggccaaga agcgtgatca ccggaagctc ggcagggaac
    59401 tcgacctgtt cagcatccag gaactggccg gtccggggct tgcgttcttc caccccaaag
    59461 gcggagtcgt ccgcaagctc atcgaggact ggatgcgcga ccagtatgtc gcccgcggat
    59521 attcgctcgt ctacactccc cacgtggccc gtgcagacct ctggaagacg tccggccact
    59581 acaacttcta cgccgagaac atgttcgccc ggatggagtt ggacgacgcc gagtatcagc
    59641 tcaggcccat gaactgtccg ttccacatcc tcatctacca ggacaggatg cgaagctacc
    59701 gggacctgcc agtccgcctg ggcgagctcg gcaccgtcta ccggtacgaa cgctccggcg
    59761 tcatgcacgg gctgttgcgt gtccgcggct tcacgcagga tgacgctcac atcttctgcg
    59821 cgcccgagca gatcgaagat gagattgtga actgccttca gttcgcggtg gacgtgctga
    59881 gcaccttcgg tttcgactcg tacgaagcgg agttgtcaac ctgggacggc ggcaagagcg
    59941 ggaagtacga cggctcgccg gatcagtggc agctcgcaga gagcgcactc cacaaggcga
    60001 cggaccgcct gagcatgaag gtaaaagtga tacccgatga ggcggccttc tacggcccga
    60061 agatcgacgt caagcttgtc gacgccatcg gccggctttg gcagctttcc acggtacagt
    60121 tcgacttcac gctgccgaga cgcttcggcc tcgagtacat cgccgaggac ggtaaggcac
    60181 accagccgct gatggtgcac cgcgccctgt acggctcggt ggagcggttc ttcgggattc
    60241 tactcgaaca ctatgccggt gccttcccgg tgtggttggc gccggtgcag gcggcggtgt
    60301 tgccgatcac cgaccggcat gtcgactacg ccaagcaggt cgccgaacgt ctcaaggccg
    60361 ccggtgtccg cgcgcacctc gacgaccgta acgaaaaggt caacctcaaa atccgtgagg
    60421 cgcaattgca gaaggtgcct tacatgctgg tggtgggcga ccgcgaagcg cagaacggaa
    60481 cggtctcggt gcggaaccgg aagcacggcg atcagggagc caaacccgtc gaggagttcc
    60541 ttgcggcgat cagatcactg atcgagacca agtccgtttc cgagtagcgg tccccagcgg
    60601 gctgcctgtc gtcaggtact tctcgacctt cgccaacgcg gtgtcgatgg gaagtgtgct
    60661 atccaccgtc acaccctcgc tgaaaaacgg gtaaccggac acaagttcga tcacgcgctc
    60721 gggcgtcaga tcgatgcccg gatggttccc ccaacgctgt tgcgcccgct cgactgcgat
    60781 ctcgggagga accgtgcatt caatcaggta gagcgccgct ccggcccgcc cggcggcctc
    60841 ttcaaggtcg gatcggtcct ccgcgcggcc atagcacgcg ttcacaatga cagtcgcgcc
    60901 ggatgtcgcc agcaactccg ctccaaggtg catggctcgg tacgcgacag cccggtcctg
    60961 gcgcgtatgc gccgagtccg gcagcaggcg gacacggacc gcatccattt cgagatgtac
    61021 aatcccccgc cgcgaggcca gcagatcgcc gagagtcgac ttgccggaac acggcggtcc
    61081 ggaaacgacg atgatcattc ctcggtcgga gtgaatcgta gtgtctcctt tgcctcaatg
    61141 ttgcggaact gcatacggaa actccgaccc tcgaggtacg acttccattg gtcgcggtaa
    61201 tgccgcgaga gcacttgtcc ggactggccg aaaggaatgt tcaggacgga gcggtcgaga
    61261 tccgagaagt cgacaaccat cctcattgac gggccgactc gcggcacgaa gcggacctgt
    61321 ttcaccgtcg tcggtgcgcc tccgaatgga accggtccga agttgaagta cttcccagca
    61381 agcggaagtt gcccggcaat gggatggttg atacccagct ggttgtaacg cccgtagtcc
    61441 catttcgtca catcgcggcc ttgtgtacgc tgcccttccg cgatcgcgtt tgccaggttg
    61501 ctgacaagaa gcttgtccca atcgccggcc cattccggcg ggcgtgtgcg aagcaaccgt
    61561 tcaacgacgg cccgcgacat ctcgtaggaa tagacgaccc ctttgcccgg agaagcgcgc
    61621 tcggcgaggg ccctgcacac atgctggctc gcgagttcgg cgatcaacgc cgccgccgaa
    61681 ccgttccgca tctggccgtc gaaatctcga agtagcttcg ccgcctcatc gaccggagcg
    61741 cctttcgcgc cgcgacggcc acatgccgcg gcgatctcgc gggcgaggta gtgatgaaac
    61801 gccgagtact cgtccgtctg gagccgcagc gtatcctccg tcgaccatcc tttccgggat
    61861 gacagccgcg cacgaatctg gttcgcgcgg tagggagacg aaaaattccc gccgactcgg
    61921 taggggtaat cttccgggaa cggattctga ttcgcgctga tgatgatacc cgaagccggg
    61981 ttgaagacgg acggaagctg ctcaaacgga atgaaccccc gccattcgaa ctcaccgaag
    62041 ctgccatcca ccgggacatc gccggagtag ttcgcacgaa tcgggaaccg gccggctgct
    62101 tggtagccga tgttgccgtc gagatcggca tacacgaagt tggagccggg ggctggaaga
    62161 cgcgccaggg cggctcggaa ttcgccccag ttgcaggcgc ggtcgaggtc gaggatcgga
    62221 tattgaaacc cacccgattc ggctggcgcc caacgcaccg aaagataacg gccgccttgc
    62281 gacagaaaga tcggtccgtt ccgggtgacc caggttacca gttgctccgg tcttccgtcc
    62341 ctcacccgga tgacctcacg ctcgggacgg gcagtctcga cctggccacg ataaacgtag
    62401 cttccgccgg caggatcgaa cttttcgaca tagaggtcct gcacgtcaaa ctgcaagttc
    62461 gtgaccgacc aggcgatgcg ctcgttgtgg ccgatgatga ccccgggcag cccgggaagc
    62521 gacactccgg aaacgttgag tcccggcgcc ttcaggtgca ccatgtacca agttcccggc
    62581 accgagtact cgaggtgcgg gtcgccggca agcagtgcct tccctgtggc ggtgcgtgat
    62641 cccgcgatcg cccatgcatt ggagccgggt tggaactccg agccgctgcg cacgggaaaa
    62701 agtgcttcga ccttttgacg gtctccgccg gcaagcaggt tctccttgcg taactcgatc
    62761 ttccacattg atgagagcat gctctccatt tgcagcgcgg acagaatcgt atcgaccacg
    62821 ctccacgggc gcggctcgta ccgcagcagc gtaaactcga tgggaagctg tctgcggtgc
    62881 gtttcgatga agaagttcac gccgccggcg taagcggcca acgccgtacg atcgttctga
    62941 gtgatggcct tgtagtgctc ctcagcgaca cggcgcaccc gcatctgccg ggcttcgcgg
    63001 tcggaattca gcgcacccgg tccgacaatc tcggccagtt cgccggccgc gaggcggcga
    63061 atcgcatcca tttgccagag gcggtcttgc gccgtcacga acccttgaag gaacagagcg
    63121 tcctgctcgg aggctgcgga tatgtgaggc acgcccagag cgtctcgtgt gaccgtcgcc
    63181 tgggcctcga tgggcgctgt aagcgagccg gaggtcaccg gtagcggccg ccagacgaac
    63241 caatacacac cgatgagcgc ggcggccagc gcaaccgcaa tggccaagtt tatatacttg
    63301 agaatccgat ggctggccac tccgcaattg tactgaaaag cgtccagccc gccgcccggt
    63361 gacctcaatg gaagaagttt attacggtta ccgttttctt gtgctggcgc tcatcatcgg
    63421 cgcgaatgcc ttttttgccg gctccgaggt cgcgctgctt tccgttcgtc cttcgcggct
    63481 gagagaactc gctgaggagg gcagcgtcgg agcgcaggca gcgctcagag tgctggccaa
    63541 ccctgagcgg ctgctatcgg tggtccaggt cggcgtgacg cttgcgagcc tgggccttgg
    63601 atgggcgggc gaggacacgc tctttcgctt tattagtggc gtgtttcatc cggtgctcac
    63661 tccggcgacc gagaagatcc tccacgcagt gagctttttc ctggccttcc tggtgatggc
    63721 cttcttccat gtcgtggtcg gcgaagtcgt acccaaaaat cttgcgatag agaaagctga
    63781 ccggcttgcc atcctagttg ctccggcgct gctgttgttc tacagagtct cggagccttt
    63841 cgtttatgtg atcgagcgct ccgccgcagt tctttccagg atgttgggac tgcggggcga
    63901 gccgcgtgga ggcgggcatt ccaccgagga actgaaactg atcatcagcg cgagccgtgg
    63961 ggaaggcaac ctgccgaagt tcgaggagaa cgtcatccac cgcatcctgg acctgccgga
    64021 cttagtcgtg cgcgaaatca tggtgcctcg caacgatatc gtctcggcgc ctgttgattc
    64081 gagcctcgac gagatcctcc gcaagatgat cgagcaccag tactcgcgct tgcctgttta
    64141 cgaggaccgc cccgagcaga ttgtcgggta cctccatctg aaagacgtcg cccgcgcgag
    64201 agaggagctt cgaacctccg gactgcctcg gatgccttcg ccaaggcttc gcctccggtc
    64261 cctgttgtac aagcccctgg ttgtgccgga gaccaagccg gcaagccagc tcgtggacga
    64321 attccgcgag ggccatgttc acatcgcgat ggtcgtggac gagttcggca cgattgtcgg
    64381 tatggtcacc ttcgaggacg tcatcgagca ggtcttcggc aaaatcgagg acgagcacga
    64441 ccttcgcacc cagccacccg cgttggaagc gcttgccgcc gacgtcgaag gcacgatcac
    64501 gatccgcgac ctggaaatgc agtacggaat cgagttgcct gccggtgcgg gcttcgagac
    64561 cctggcgggc tttctccttt atcacctcgg ctacattcca aggcctggcg acgtggtcga
    64621 gtatggtgga cgccgtttca ccgtactcga aatggagcgg aaccgcatcg cgcaagtccg
    64681 gatcgagaag gtcgagggag acactgcctg aaggccctca acgcccgccg cggagatacc
    64741 agcgggccag gacgtccatg gcgggcttac cccacggagt gtgagagccg tcctcgggtc
    64801 cgccaaaacc gttgctaccc accttccacc agtaaacacc atggaaccag ggcttccggt
    64861 agaacgcctt gaggacggca tcgtagcagc gagcctgctc ttcgagtgac agctttcggg
    64921 gcgtctcgtc ccacggctga cggttcgacc cctcgagact ggcgaagccg gcttccgtga
    64981 agatgaccgg acggtggtat ttccggtgga ccgattcgat tttcccgacg acagcgcccg
    65041 tcgagaggtc gtcgggcagc ggatagtatt ggttcaatcc gatgtagtcg agagcgtccc
    65101 aaaacggcag attctcgaat tcctcgcccc agttggcagc atagacgagg ggacctgcgt
    65161 aaattgtgcg aacccgtgcg ataaccgccc gccaatgctt ctcgtgacgg gtcacccggg
    65221 agagttcgac gccgacacaa aacacgtcgg cccgaatccg tgaagcgagt gaggcgtagt
    65281 ggcagatgaa ggcgtcgtac tgagtgaacc acggctccaa atcggcgggc gaggagaagt
    65341 cgaggtcacc ggggtagccg cggcggaccc aaagttgcgg tttcagcaat accttgatgc
    65401 cgcgttcgtg ggccaggcgg gcaacccgca cgaccgaccc gtccttctcc catatgcgat
    65461 tgccgccaaa acggatgacc ggggtgtcgc ggcgagcgaa gccgtaagga atgatcgcga
    65521 ccgcgttgat accgtacgca ggcagcttcg acaggattgc cgcggagcgg tccgaggcgt
    65581 aaacatcggg ccattccgaa gtgaagttca cgccgcgctg gaagaagagt ccggggccgg
    65641 gtgacactcc cagcaagaga agacaggccg ccatgagggc ggcgacggcc gcgtatctca
    65701 tggaattcaa taggggctga tggcttggag ttcctgggcg cccgcctggc ggagaagccg
    65761 ttcgacttcc ttcttcagcg gcgacacgct cttgccgaag atcatcttgt aggcctggca
    65821 aaagtagtcg aggtcctcga aattgtgcct ggggccgtgc cggaacttcg gacaaccggc
    65881 gtggcagatc gactggtact cgcagacctg gcactcgggg tgggcgatcg tctttttgct
    65941 cgcgaaactg taccggcgct ggcggcgcgc gatctcgggc cacgagtcaa tcaggatatt
    66001 cccgagcttc cagtctttct cgacaaagaa gtcgcagggg tagatgtccc cgttgtactc
    66061 gaccacgacg tagctgtcac aggtctcgtg catcgtgcaa gtgccgggtt tctggccggc
    66121 gagtgcttcg gcgaggttgt caaagaagcg gatgcgcgct ttccggcgct cgggccacca
    66181 taggtcgaaa gtctcgcaga gaaaccggcc gtactgttcc ggcgtgatcg tgaacggcag
    66241 gcggttgccg tttccgtcga actcggccag cggaatgtac tgcacgaagt cgattccgag
    66301 actgcggaag aaccgataaa tctccttcgg cttctcgaca ttcgcttggc tgagcacgca
    66361 gaggacattg aaatcgacct tctccttctg gagcagctcg attccctgca tgactttctt
    66421 ccatgtcccg cgcccctgct tgttaaaccg gtacaggtcg tgaacctctt cggggccgtc
    66481 gatcgaaact cccacgagcc agttgtacgt gcggaacaac tggcaccagc gctcatcgat
    66541 caggatggca ttggtctgga tggcgttgct gacgttttgc cccccgcggc catgctgctg
    66601 ctgcaaatcg accagctttt cgaaaaacgg gagtcccgcg agggtcggct cgccgccctg
    66661 gaaagcgaag acgctgttcg gataggaata gaacaggtag gtgtcgacca gccgttcgag
    66721 ggtgtccgct gtcatgcggc gcgcgggaag ttccttgtat gggtccgcct cgcggtcgag
    66781 atagaaacag taggcgcagt cgaggttgca gaccgccgaa gccggcttga tcaacagcga
    66841 cgtgatccta ggcgtcgttc ccagcccctc tcccagaatg gggaaaggcg ctccgtttcc
    66901 cataccgtga gtgtagcatc cggtcacggg acagcctccg gttccacagt cacagcccac
    66961 cgttccctga cggtgtagga ttgcgggtag cacgagccat gacgccaaga gagttggtct
    67021 tcagcgtgcg ttccgaactt gcggcgagcg ccgacccaaa gttccgggag ggtttgatgt
    67081 ggttcttcaa ggagcccgtc gatccctacg gggtacggac gccacaggtg cggcggatcg
    67141 ctgcttcggc gtaccgcgag gtcaagaagt ggcccgcggc acagcgcaac gcgttctgcc
    67201 gggaactttg ggcgagcgga aagatggagg aaggcggagt cgccatttgc gtctaccagc
    67261 gattccgaaa gcagtgcgcc gcccgcgagt tccatctgtt cgagaagtgg atcgatcgtt
    67321 acgtggggaa ctgggcacac tgcgacggcg ttgcgacctg gctgattgcg gcgttgattg
    67381 ggaacgagcc gggattgatc cggctgctat tcccgtgggt tgcgtcgaag aaccgatgga
    67441 aacggcgcgc ggccgtagtt tcgttcttac aggaggcgaa gcagggacgg aacacggagg
    67501 ccattctgaa agtcgccggc gccgttctgg aagacccgga cgacatggtt cagaagggcg
    67561 tcggctgggt cctgaaagag gcgtatccga aacgcccccg cgaggtgatg gatctgctgc
    67621 gggcccggaa agagcgtgcg ccgcgactcg tgctgcgcat cgccgccgag aagatgaccg
    67681 cggcgcacaa agccgagatc ctcagccgag cttcggtggc acgtcgttga gcgggccgag
    67741 ccactccaat tcgcgttcga tgtcgcggtg catccggtac tgtttcttga ccgttgcaag
    67801 atcggcgccg accacgtcga tcttcgagag atcgtactgc cccagaccga cctgccagca
    67861 gtattgcagg taggcgggcc actttgcgtc aatgcccatc gcttccaggc cgacccggtc
    67921 ggcggcgatg taatcggccg aagcgattgc gacgcgcgaa gccaccgggg tgccggaggc
    67981 cgggccgttc ccttccatgc cctcaaaacc gtcgatgagg gtggctcccc agaagggctt
    68041 cattttctgg gcagtcagca tcatgttgta gtgggtctgg cggatgcccc cgtggtattt
    68101 ccgtttgtcg ttccacttcg gctcgcccgg agcgctgcgg agcggtgctc ccagggtcat
    68161 gttcttgatc gaaagcgtcg cgacgaccgt gttgtgtgtc ttcaggattg cggcgcacaa
    68221 cacgaaggcg tcgggatcga gcagacgagc ggcaaggcgc acgggctgca cgtggaggtc
    68281 cggactgagg agcgggatga cctcgtactt gccttcgcgg ttgaggtcca ccagcgagac
    68341 cttctgtgac ttgcgctcgg tagccagccg gttgtagccg aagttctcgt agccctccag
    68401 ggtgtcaccg gcggaggact ccgcgatgac aaccggccct ttgaagcggg gcccgagata
    68461 gtcgaggatg ccgtggagcg tgtccgcgtg ggtagcggcg agctggttca cggtggacac
    68521 attgttcggc ttgattacca cgaacttctt ttgtttgaga accggacgaa tctgatcgtc
    68581 gatcgcgacg agcgaatcgc acacgatctt ccgccgctcc tcgccccgaa tcacggagac
    68641 ccgggaccgt ctgttgaagg gaacaaccgc ttcgggaacg tagccgccct ctagcggagg
    68701 cgcctgttgc gcgcgaagcg ccgaacccaa gcctgccccg gctacgagat gaaaaagatt
    68761 gcgccgcgtg agattcccct tcatcagacg tcctcctcgc ttataataaa gatgttagta
    68821 gctgattata cctgattttc gagaacaact atgaagacca attcctcgcg acgcaacttc
    68881 ctggcggccg gactcggctt gcccgttgcc ggcatcggcg ccgtcagttc gagcggcccg
    68941 gcgatcaggc cgtctgcttc cttgccggct cctggcggcg aaatccgcta tcgaacgcta
    69001 ggcaagaccg gcatgaaggt cagttccgtc ggattcggct gcatgatcac gtccgacccg
    69061 agcgtgatcg agaaggccgc cgacatcggc atcaaccttt tcgacaccgc gcgcggatac
    69121 cagggaggta acaacgagcg catggtgggc gcggcactca agacacggcg caagaatatc
    69181 ttcatctcaa cgaagacggg agcctcgaac aaagaggggg cgcttgccca cctggaaacg
    69241 agccttcgtg aactggggac cgactatgtc gacatctggt acctgcacgg gaggagcagt
    69301 gcgagccagc tcaccgagga cctgatcgcg gcacagcagg aagctaagga agcgggtaag
    69361 atccggttcg ccggcgtcag cattcacagc gggcacggcg aggtgatacc ggcaatgatc
    69421 cggtccggga agttcgacgt ggcgctgacc acctacaact tcacgatgga gaagagcatc
    69481 gaggggctga tcgattccgc ggcaaaagcg gggttgggtg tcgtcgcgat gaaagtcatg
    69541 gccggcggca accgcagggt gaaaccgggc gacaagatgt acgacaccct ccagcgggac
    69601 ggcgcgatgg tcgcgatgct caagtgggcg ctgcgaaacc agaacgttca cacaaccatc
    69661 ccgagcatga ccgacatgga ccaactcgac gacaacctgc gcgccatgac aaacgcgttt
    69721 actgcgtccg atgagaagct gctgtcggcg cgactggagc agattcgccc gtactactgc
    69781 cggatgtgcg gagagtgcga tggaacgtgc ccgaagggcc tgccggtggc tgagattctc
    69841 cggtatgtga cgtacgccga tggttacggc cagttcgcac tgggccggga acacttccta
    69901 tcactgcccg aagagatgac gcaggttcgc tgcaaccttt gcccgacgtg ctcggtggag
    69961 tgcaagttcg gcgttaacgt ggcgcgacag ctcagccggg cgcaggaact gttcgcatga
    70021 gacgcagaat tttccttggg gtcctggccg ccgctgcgac gctgtccgcc cagaagccgg
    70081 attgcagcgt cgttcccggt tgggaacaag ccggtcccgc aaggatgtac acggcggaca
    70141 acctgttcga ctacatgaac ggcaacgccg aggggtacgt catctactcg ttccacaaga
    70201 tggacggagt gacgtgcaag tcgggtgaga acacgttcgt atttgacgtc tccgagatgg
    70261 gcagccctga g
//
LOCUS       DATIFQ010000360        48870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1650527, whole genome shotgun sequence.
ACCESSION   DATIFQ010000360 DATIFQ010000000
VERSION     DATIFQ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 48870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48870
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650527"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VLE22_17390"
     CDS             complement(<1..271)
                     /locus_tag="VLE22_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16232.1"
                     /translation="MREPELDRSLPAVIERYSAPSYVPPAMPVDVEAEEPSVPLSHYL
                     WILKRQRWKIVAFVGMSVVGTLIVSARLTPVYESTATVDVDRQTPT"
     gene            complement(314..1297)
                     /locus_tag="VLE22_17395"
     CDS             complement(314..1297)
                     /locus_tag="VLE22_17395"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HSB16233.1"
                     /translation="MTHFARRRWCCRLAFAVCFASIALCGIAVAQVRSVPEAVVANLP
                     AQKIGPNDLIAVSVYDAPEFTRTVRVSAEGLVRLPMLKRRVRADGLLPAELEAALAEA
                     LEAEQLLVEPVVTVTIAEYHSRPISVAGAVRNPVTFQATGPMTLLDALTRAGGLSQDA
                     GQEILVSRTQRGEDGKTSTVVQRISVRGLIEAADPELNLKLAGGEEIRVPEVGKIFVV
                     GNVKRPGAYPVQDTSETTLLHVLALAEGLAPYAAKQAYVYRRELSTGARNEIPVALSE
                     ILKRKAPDVPLQANDLVYIPDNSGRRIAVNTLEKIAGFGTATASGLLIWRR"
     gene            1869..2699
                     /locus_tag="VLE22_17400"
     CDS             1869..2699
                     /locus_tag="VLE22_17400"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HSB16234.1"
                     /translation="MQLYIAAGIGLAVLVTFAFLAARRLADERRLRAIWKSLEGDGRG
                     AAFHPDMVTGLPEPARRYLLHAIKPGTPLASSVALRMKGKLRPKATVPWTEMKAREII
                     CVTKGFLWKAKVRRGLSRVSGADHYADGQARVRFWSWGVFAAVRMEGEDTTKSAIGRL
                     AIEAVWLPSALLPQSGATWEAVDNHAAKVTLKIDGQPTTLVVNVEPDGKLRRSIISRW
                     GGVGESGEAVWMPFEVESHEEREFGGYTIPSFCTAGWWVRSDHFFEFFRANVESAEFR
                     "
     gene            2745..6950
                     /locus_tag="VLE22_17405"
     CDS             2745..6950
                     /locus_tag="VLE22_17405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSB16235.1"
                     /translation="MPLPGFDPLIDEWFRGRFAKVTEPQLLGWPEIRAGRDVLISAPT
                     GSGKTLAAFLICLDELVKSARGGSLPNRTEIVYVSPLKALSNDIQKNLEAPLAEISAL
                     ADAKGLRLAPIRTAVRTGDTTVRERLRMLEQPPHILVTTPESLFILLTADKSRKILQH
                     AKAVIVDEIHAVAGDKRGSHLALTLARLEALVREASGGTLQRIGLSATVKPIEEVARL
                     LSSSAGIVNVGHRREMHLAVESPKDELGAVATLSLWEDIYDRLAELIRTHRTTLVFVN
                     TRRLSERVTHHLQERLGTEAVLAHHGSLSRRLRLDAEQRLKNGELRAVVATASLELGI
                     DVGTIDLVCQIGSPRSLSVAFQRIGRSGHWVGAMPKGRLFVTTRDDLIECAAVVRAVR
                     RGELDRLEIPEAPLDVLAQQIVAAAACGEWREDDLFELARGTWPYRSLARKDFDAVVE
                     MLAEGIATVRGRSGAYLHHDRVNGRIRGRRGARLAAITSGGAIPDNANYLVVAEPEGA
                     TVGTVDEDFAVESSAGDVFLLGTVSWRIRRVEQGKVRVEDAHGAAPTVPFWRGEAPGR
                     TAELSSEVSQLREEIAARPKEEAVEFLLHECSLDRRGAEQSVEYVHSGRAALGALPAQ
                     RTVVAERFFDEAGGMQLVIHAPFGSRINRAWGLALRKRFCRSFNIELQAAATDNGIVI
                     SLSEQHSFPLEIVFVFLKPENVAYVLTQAMLAAPLFTARWRWNASRALAILRFAGGRK
                     VPPPIQRMRAEDLIAAVFPDQAACAENLDGDIRIPDHPLVTETVRDCLHEAMDVDGLR
                     AVIAAMESGEIRTLARDTPEPSPFSHEILNTNPYAYLDDAPLEERRARAVQVRRTIRA
                     DVMDGVGDLDPAAIAEVAEDSWPSVRDADELHDALLTLVTVPPSAEWEPHFGALVAAH
                     RATTLETGGRPFWVAAERIDLALAVHSDGGLAPWIETVLPSSPIPGTVEECVAAILRG
                     WLESCGPVTAFGLSGRLAMPLDLVTSGLLRLEAEGQVLRGRFTPDLPNGELEWCNRRV
                     LARIHRLTLGKLRREIQPASTSEFMRFLCRWQHILPGRQLHGLEGAFQIIRQLQGYEI
                     PAAAWESFVLPRRIANYSPELLDRLCLSGEVMWGRISRHPAFDDPEPGPRRVRPTRVA
                     PVAIFMREDAALFLGGLEKNHADTPLSHPARELLAELELRGASFFGELQRATGRLPSE
                     VEDGLWELVAAGFVTADGFENLRALLDPKRRRGEGRGRHARPRHAAGRWALLRPHIPS
                     PDAEEAHARQLLRRWGVVFRDLLAREASAPAWRDLLVILRRMEAKGEIRGGRFVDGVL
                     GEQFALPEAVELLRAVRRAGKSGETVEISTADPLNLAGVILPGARISALAGGTINLRD
                     GVPDAVCGASSGGGPVTAVVQST"
     gene            complement(7362..7907)
                     /locus_tag="VLE22_17410"
     CDS             complement(7362..7907)
                     /locus_tag="VLE22_17410"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSB16236.1"
                     /translation="MKHFWLKFACIALVVAVALPSIGLANRAASTASERISHEVRHEI
                     LMLPYYGVFDFIAFKVDGSKVTLIGQVTRPTLKSSAERVVKDIEGVESVDNQIEVLPV
                     SPNDDRLRLALYRAIYRSSALERYAIRSISPIRIIVKNGNATLEGLADSPGDKNLAGV
                     LAKGVPGVFSVTNNLQVATSK"
     gene            complement(8006..8851)
                     /locus_tag="VLE22_17415"
     CDS             complement(8006..8851)
                     /locus_tag="VLE22_17415"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16237.1"
                     /translation="MKTLFWLLLAFLLIQQACGPYRLAASRSRVLDRFQQQRKSRVIA
                     MIHRQDTVSVLGVPVSSSIDIEDSEAILRAIRLTPQDQAIDMVLHTPGGLLLAAEQIA
                     KALVEHKGKVTVFVPHYAMSGGTLIALAADEIVMDPNAVLGPVDPQIGDMPAASIVRL
                     LQAKRPEQISDEMLVLADVAAKARIQVAYFVGDVLLKHLPKDKAGALAVALSEGRWTH
                     DFPITVEAARELGLPITTNMPETIYELMDLYPQSGAGRPSVLYVPLRQAPGRHGERTG
                     QGPRG"
     gene            9028..10641
                     /locus_tag="VLE22_17420"
     CDS             9028..10641
                     /locus_tag="VLE22_17420"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB16238.1"
                     /translation="MTRRAVFRASMLATAGGILARGDPAARPNILWITCEDTSPDLGC
                     YGDSWARTPNLDRLASQGARYTNAFSVYGVCAPSRSSIITGMYPASIGTHHMRSQGVP
                     PPYVKCFTEYLRAAGYYCTNNSKTDYNFPAPLTAWDESGNRAHWRKRPKDQPFFSVFN
                     ITTTHESQIWPANNAKATAILAPEERHDAAHAKVPPYYPDTPLTRCDWANYYDLVTAM
                     DKQAGGLLRQLENDGLAENTIVFFYGDHGRGLPRAKRWAYEASLHVPLIVRRPGTIAP
                     GAVTDRLVSLMDLGPTLLSIAGVEIPKYMQGRAFLGDRAAQPRKYVFGARDRMDEAYD
                     LIRAARDGRFHYIRNLQSCKPYAQYIDYMETGQTMKEMRRLNKASQLQGPQRLFFVPE
                     KAPEELYDIPSDPHCIHNLAAAPAHQNTLKRMRAAVDEWMSEIKDLGSVPEDQLKERM
                     RPGGKWSVTAAPKITPDGGSFAAPVAVRLACPTEGSSIAWTTEQSEGSRWNLYGREIA
                     LDRTATLRVKACRLGWEDSPEVRAEFKIG"
     gene            10646..11242
                     /locus_tag="VLE22_17425"
     CDS             10646..11242
                     /locus_tag="VLE22_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16239.1"
                     /translation="MRLVLLTTSVLAISGCSQAPAPAPKSAGQPAAEVKISQFYANTG
                     EVERGTPVTICYGVENARAVRIEPPVETLKPAVSRCFSVTPDHGATYTLIAEGSDGKT
                     VTATFRVNVKGRTAARPELVQLFGSSSSEIQAGQTATLCYTVDDATSLRIEPGVPTIK
                     DLHSGCILVRPNESTVYTLTAVAADGRTESRKLTITLK"
     gene            complement(11258..12724)
                     /locus_tag="VLE22_17430"
     CDS             complement(11258..12724)
                     /locus_tag="VLE22_17430"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB16240.1"
                     /translation="MTRRDALRTLGGAAAAGFPNIAKPQIKRPNVLFVMTDDQRWDAM
                     SCAGNRILRTPNMDRLAEGGVRFSEAFVTNSLCSPSRGTILTGLYSHTHGVTTNESGS
                     HYFRVGTVNFPMLLQEAGYYTALVGKWHIASEPAGFDHWLILPGQGVYVDPVMISKGS
                     RMQFRGHVEDVIGDQALETLKSRPKDTPFCLLYQFKAPHRTWIPARRFEKMYDGITIP
                     EPPTFDVDLSGRPAAVRNSDMQIRDMIDFQNRGVPASLPPKERKRRNFQLFLKNYYRT
                     LLGVDENLGRVLDYLDKEGLAENTLVIYTGDNGFFLGEYGMFDKRLMYEPSIRVPMLV
                     RYPAAIRAGQVDREHMVLNNDIHYTILDYCGVKRPAALPVHGESWRPILDGRQTPWRD
                     SWLYEYFEYPGYHCAGKHRGVRTKRWKFIHYIQEPQEYELFDLQNDPQERRNLYSDPA
                     HRQQADEMRKELERWRDRTGDDRSADGTPTLPCVSPKS"
     gene            complement(12740..13543)
                     /locus_tag="VLE22_17435"
     CDS             complement(12740..13543)
                     /locus_tag="VLE22_17435"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSB16241.1"
                     /translation="MHPGATPPVRMGRMVWEKELEVARAAATQAGEIALRYQAQGVEA
                     EAKADESPVTVADRECEKLISRILLEAFPDDGLLGEEGALKESRSGRRWIIDPVDGTR
                     DFVRGSDTWGMLIALEADSEVVAGVCHQPAQKELYSASRGAGALRNDDRVRVSEITSP
                     SQSVLCVNGINDIADLPFAGRLLEFTKQFWAVRSMGGCQDAMMVAGGRAEAWIEPSAK
                     PWDLAPLKIIAEEAGARFFNFDGGSSIYGGNCILCVPALEELMRQFLGL"
     gene            13558..15345
                     /locus_tag="VLE22_17440"
     CDS             13558..15345
                     /locus_tag="VLE22_17440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSB16242.1"
                     /translation="MGHRTALTALEEAASQFGKLAALCQPAGEGKHRVYSWIEYRDIA
                     REIAAGLRSLGIGKGDIVALDSETRAEFYLADLGILANGSIAAAMYGSYPAAILVHTL
                     RSCGAKAVFVEDPDTLRVLQAAGDPPLAVDWILLTGTAESALSLEDLRERGRRSIESD
                     PGLPARIASELHPSDPAILYLTSGATGEPKMALASHAALLANMEMGPAVLGLTPEDST
                     IAFLPSAHIAQRVVVELLPICCGMPVWFVESLAKLPPALRSVRPTIFLAPPRFWERVY
                     TSVCTEIQKRSPVVRKLFYGALGLGIDVHRREREGKSVPRWIRNTLKLADRLVFRKLR
                     ARFGDRLRLPVSGAAPLGKELAEFFEVIGMPIVEGYGLTEGGILTLNPLDRPRPGSIG
                     KPLPGVEIRLAPDGELLVRAPCLFTGYFNDPKATSEVLRDGWLYTGDTAEIDAAGFIY
                     ITGRKKEVIISSNGKKIYPVRIEGLFKLEPIINHVVPLGDGETFVAALLTVNAGVAET
                     LKGAEHLRGRPMSEIAAAPPVVAEVKRALARVNAQLAPFEQIRRYRILDRELTIQDGE
                     LTATLKIRRSRVLSNYRAAIEELYSGREN"
     gene            complement(15356..15790)
                     /gene="tsaE"
                     /locus_tag="VLE22_17445"
     CDS             complement(15356..15790)
                     /gene="tsaE"
                     /locus_tag="VLE22_17445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSB16243.1"
                     /translation="MSARAYRTHSEEETVAFGEALAARLPARATILLIGNLGAGKTTL
                     AKGIVKGLGAADPEEVSSPTFTLIHEYGEDRKVYHVDLYRIERPREAASLGLEELLER
                     ESVVLIEWGERFPELWPTDRIEIRLDRIDEDTREILVAGLAE"
     gene            complement(15759..17309)
                     /locus_tag="VLE22_17450"
     CDS             complement(15759..17309)
                     /locus_tag="VLE22_17450"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HSB16244.1"
                     /translation="MKVLTAAQMRAVDRRTMELGIPGLILMENAGHRVVEFLRETFAP
                     LANHRIVILCGKGNNGGDGFVVGRQIHTRFRPRSLDVVLASEPEELRGDAAENFRMLR
                     VCGCPVAMEITPRMREATIVVDALLGTGLKGAAAGRMLELIREINTGFPHAMIVALDL
                     PSGLDSDSGTVPGDAVKAGHTITFTAPKAAQVLPPACDYVGELRVSPIGSPPELYEND
                     ASIFLSLVEPLLFQDLLGPRAPGAHKGDFGHVLVIGGSRGKTGAAAMAGLAALRAGAG
                     LVTVASAESAIPVIAAHAPELMTEPLPETETGAISLRAFDYERLAGLVRGKEVVALGP
                     GLGTHPETVAVVQRLVEEVEVPLVIDADGLNALAGVEFGGRGLVLTPHPGEMARLAGS
                     STAEVQSDRVGAARAFAVSRKATLVLKGQRTLLAFPDGNVWINPTGTPAMATGGSGDI
                     LTGLIAGLLAQFPNCENAAVAAAVFLHGRCGELGAAAFGEKALIATDMLEFLPEAMDE
                     CAGVSDAL"
     gene            complement(17306..18316)
                     /locus_tag="VLE22_17455"
     CDS             complement(17306..18316)
                     /locus_tag="VLE22_17455"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HSB16245.1"
                     /translation="MSRVTRRLGYVGLVVLCTLLVGTVGYIVIEDYPPFDAFYMALIT
                     MTTVGYREVHVLSTQGRVFNSVMIFFGVTTMFFAIGMMTQTIIELELAEFFGKRRVKR
                     MIDKLENHYIVCGFGRVGRNASLELNQSGAPFVVIDRNPERVESAIRAGMLAVQADST
                     RDQTLRDVGIVRARGLVAALATDADNLFVILSARNLNPMLNVAARVDEEEAEEKLRRA
                     GADIVFAPYSNAGYQLAQALLRPHVFRFLDFTSRSIGLDVAIEQVRVAEQSEFASKTL
                     KQMQIRRDVGVIVLAIRRADGSMLFNPPAEAEINGGDFLIVMGEPPGLRRLEVLMAGA
                     QS"
     gene            complement(18324..20312)
                     /locus_tag="VLE22_17460"
     CDS             complement(18324..20312)
                     /locus_tag="VLE22_17460"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSB16246.1"
                     /translation="MPVSSPLKSFYRKLRWPEKSFLALLVLYLILRFASPAGSLILLV
                     QLGLTASGLVAAVRLVRIWMRKTIWRLRNRLIVAYLFIAVVPVAVILVLVGIGAYLLT
                     GQMALYLVITELERHSSSLVGPTIGLIATPAPEREDRIRWMAPYLAKRFPGLEVLIRE
                     GGSVWRYPEESKLESPPHGWGEKSGFLSRGGTFYLWAHSESRAIQGTLLTRLDRDFLD
                     GLVPSLGHVRLISRSVLEGTRRASTRSEKANVGEAPPAASGVPPAVNRFDIEVSWFAW
                     VPVAMWESPGNTTIAALDVQTRVSAVVRTIFSQRLGSGQDDLNTAFLIVAILFLVVEL
                     VSLIIGVSMTGKITEAVHNLYEGTQRVMHGDFSHRIDVRGSDQLADLSHSFNRMTENL
                     ERLVRVEKEKERLQSELEIAREVQNQLYPRTVPSMPGIELTALCHPARTVSGDYYDYL
                     PLLESKVALAIGDVAGKGISAALLMATLQSSLRTQIRACLEATAAAAAAPGGGNGHAE
                     IPTSMLVSQLNQQLYSFTSAEKFATFYFGIYDDRKGELTYTNAGHPPPIVIRDGEVHR
                     LETNGMVVGAFPFAEYGESRIQLWSGDLLVCFTDGITEPENEYGEMFGEQGLIDVLLK
                     NVWRSSADIITSVIAAVHEWTNSPELQDDMTLLLAKRK"
     gene            complement(20399..21445)
                     /locus_tag="VLE22_17465"
     CDS             complement(20399..21445)
                     /locus_tag="VLE22_17465"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSB16247.1"
                     /translation="MFHKVLLPATLLTLAAVGTYAQAAGKMVGGPYVVRVGPDRATVA
                     WVLEFTEGGEVVLRAQKLTISGLTPGKTYHYDVSGQDEGKGYFKSAPDGASPFRFAVF
                     GDTRSRHEVHQNVVDALAKSEPDFVLHTGDLVADGRKTEQWPVFFGIERELLRKTAFF
                     PALGNHERNDPRFYEFFDIEKPYYSFRWGNAYFVLLNSDLGNTASNEEERKAFWEEQS
                     QWLEAELEKSREADLRFVVFHHPPITAVKRRQGGKHPVQEWMPLFEKYKVTAVFNGHD
                     HNYQHHVRNGVHYIVTGGGGAPLYEVDAPVPGVTQTVEKIEHFVVIEVEGSKARLEAK
                     ALDGRLIERIDFNP"
     gene            complement(21460..22026)
                     /locus_tag="VLE22_17470"
     CDS             complement(21460..22026)
                     /locus_tag="VLE22_17470"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HSB16248.1"
                     /translation="MRRYYITDRKPLGGVAPLLGAIRRALANGVEMIQIREKDLGTSD
                     LIDLVKAVLSLPNPHGTRILVNSRTDVAMVCGAAGVHLPSDSVAPARVRAIAPPGFTI
                     GVSCHSADEVRLAGEEGADFAVFGPVFSTPSKAVYGPPQGLERLREACRGLRIPVYAL
                     GGMNAGNAALCLDAGAAGIAGISMFQSS"
     gene            complement(22029..22610)
                     /locus_tag="VLE22_17475"
     CDS             complement(22029..22610)
                     /locus_tag="VLE22_17475"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HSB16249.1"
                     /translation="MRDFREYEAMAEMAHGHMCAGQILGLRMAVYGLRLLGIEDPTGK
                     DRKRLVTFVEIDRCATDAIGVVTGCRLGKRALKFRDFGKVAATFCDLQENRAVRLVAL
                     ESSKARAAERYPEIANRNLQQMKAYRELPDEELFATTWVRVALGPEELPGYKAPRTVC
                     AECGEGINFKREIVRDGKTLCKACAGERYYEPL"
     gene            complement(22628..23713)
                     /locus_tag="VLE22_17480"
     CDS             complement(22628..23713)
                     /locus_tag="VLE22_17480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSB16250.1"
                     /translation="MINARIRKRFPAGPDSAEFSLDVEFRAASGITVLFGPSGSGKTM
                     TLEAIAGFVKPDEGRILLDDDILFDGASGVNLPPQVRRCGYVFQNYALFPHMTLRQNL
                     EFAAVCASKQRRPRLERHRKVNEMLEQFHLAEVAGRRPHEVSGGQKQRCSIARAMIGA
                     PKALLLDEPARGLDAPLRTELYDVLRQLRSEFRSPILLVTHDLEECFALGDEMLVLHE
                     GRMVQRGAPRAVFDRPANVEVARLLGTFNLLPVEVLALDPVQRTSRLRFQDRDLTGPY
                     FPGRLKGDRVILCVRPEELAATPWDGKPGPNQVPTELVRYTERPHAVRLEFTGEIFAE
                     VTRAEFDKHKHNREWVIEFPAQHLRIL"
     gene            complement(23710..24375)
                     /gene="modB"
                     /locus_tag="VLE22_17485"
     CDS             complement(23710..24375)
                     /gene="modB"
                     /locus_tag="VLE22_17485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HSB16251.1"
                     /translation="MEIDWFPLWLSLRVAVLATLVALVIGLWLAYMLANREFRGKELL
                     DAAVTLPLVLPPTVLGYYLLVLLGRASPFGKFYEWLFGAPLVFTWQAAVVAALIHATP
                     LLVKSARAALESVDKSYERAARTLGASEWRVFFQVTLPLANRSILAAAVLAFARSLGD
                     FGITIMIAGNIPGRTQTMSVAIYDAVEAGNGAMARVLVLIVSAAAMFILFLANRLSPK
                     QAA"
     gene            24443..25258
                     /locus_tag="VLE22_17490"
     CDS             24443..25258
                     /locus_tag="VLE22_17490"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB16252.1"
                     /translation="MIPDTFLHTTLGRTGLPVFRLGLSASYRPGKRAVHRALDEGVNY
                     FFCYGFDTHMTGVLREVLPSNREKYVVATGAYNYIWTYQNIRRTLEKRLRRLNTDYID
                     VFHLLGVTKRPQFNDRVRDELFALREDPRVRAISISCHDRKFAGELAAQGLLDALMIR
                     YNAAHRGAESDIFPHLAAHNPGVVSYTATRWTYLLRRPHGWPRDGRVPTAGMCYRFVL
                     SNPHVHVCLNAPSNLKHFEDNLASVRQGPLDEDDMRFMHEFGDAVYRRNKWFW"
     gene            25296..26963
                     /gene="hutU"
                     /locus_tag="VLE22_17495"
     CDS             25296..26963
                     /gene="hutU"
                     /locus_tag="VLE22_17495"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HSB16253.1"
                     /translation="MTGPIRAPRGRALRCKGWKQEAALRMLMNNLDTDVAERPGDLIV
                     YGGAGKAARNWECYHAIVRELEALGDDDTLLVQSGKPVGVFRTHEEAPRVLIANANLV
                     GHWSNWDHFRALEQLGLIMYGQMTAGSWIYIGSQGIVQGTYETFAAAGRKHFEGSLAG
                     KFVVSGGMGGMGGAQPLAAVMNGAAFLGIDADPERIKRRLRTGYCDIMVTDLDEAVRI
                     LRHAVYEKRATSVGLVGNCAEVLPTMVRQGIVPDLLTDQTSAHDPLNGYIPAGLAVDQ
                     AASLRASDPQEYLRRSMDSIAIHVRAMLDLKRLGSVVFDYGNNIRRMAADQGVGDAFT
                     IPGFVPEYIRPMFMEGRGPFRWVALSGEASDIEATDDLVLRLFPSNETLGRWISLAGK
                     RVRFQGLPARICWLGYGERAEFALEMNRMVATGKLQAPVVIGRDHLDCGSVASPYRET
                     EGMRDGSDAVADWPLLNALLNTASGASWVSIHNGGGVGIGYSLHAGHVTVADGTESSA
                     GCLERVMTCDPGIGVLRHADAGYEDALLFAERAGLRLPMKEGPKQAS"
     gene            26960..28240
                     /gene="hutI"
                     /locus_tag="VLE22_17500"
     CDS             26960..28240
                     /gene="hutI"
                     /locus_tag="VLE22_17500"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HSB16254.1"
                     /translation="MSETILVRGARQLLTLRGPSAPRRGAALRDLGLIQDGSLLIRDG
                     LVAAAGATRRIENLAAARHATEIDATGRVVMPGFVDSHTHLIFGPPRLDDYERRLAGQ
                     DYHQIAAAGGGILVSVKAVRGTSSRALLRQTRLSLHRLIRHGATTVEAKSGYGLDEPA
                     ELKTLRVLAALDGSPIDVVSTYLGAHVVPPEFAGRPEEYIGWVCSYMLPLIRRRRLAR
                     FVDIYIERGAFGLDDARRYLETARSLGFGLKVHAEQFSHSGGTRLAMELGAVSVDHLE
                     QAGAEDIAMVAQSSTIATLLPGSVFHLGLDRYPPARALIDHGAAVALATDFNPGTSPT
                     CSMPMVLALACNQMRMTPAEAVSASTINGAHAAGVADRAGSLEPGKQADLLLLDVSDY
                     REIPYHFGVNPVAVTMKKGRILYRQGEVTCPEDL"
     gene            28222..29640
                     /gene="ftcD"
                     /locus_tag="VLE22_17505"
     CDS             28222..29640
                     /gene="ftcD"
                     /locus_tag="VLE22_17505"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HSB16255.1"
                     /translation="MSRRLIECVPNFSEGRDASVVDAITRAIRSVPSIVLLDRELDAD
                     HNRSVLTFAGPPDAVAEAALRGVGEAVRLIDLTRHTGVHPRIGAADVVPFIPIEGVAI
                     ADCVHLAEHVGEEIWKRFGVPVYLYEAAARRPDRVNLENIRRGQFEGLREEIQSNLER
                     RPDIGEPRLHPTAGATVVGARKFLIAYNINLNTSDLDIAKKIAKTIRFSSGGFPYVKA
                     MGVPLASRNLAQVSMNLTDFEQTPVHQVFEAVRAEAARYGVDIAGSEIVGLIPRKAVE
                     MTAEFLLRAENLRPEIVLENRLAEAMAGRGLDDFLDALAAPTPTPGGGSAAASSGAMA
                     AALGAMVAGIAKLPGYDFTPDRLFLAEAVRRDAAAYDAVVAAYRRPKDERAPFVEEAF
                     HQATAVPLEVAECVSRVRSRLESLALEAPPKVASDVEVALLLAAAALSGALANVRINL
                     PSIKDATFRRSVEQRLASIESK"
     gene            29640..30518
                     /gene="lipA"
                     /locus_tag="VLE22_17510"
     CDS             29640..30518
                     /gene="lipA"
                     /locus_tag="VLE22_17510"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HSB16256.1"
                     /translation="MLVSIAPKLERPAWLRAPAPAGDNYRELKSLVENLRLHTVCESA
                     ACPNVGECWNHRTATFMILGNVCTRRCGFCAVGKGAPLPVDYDEPRRVAEAVATLGLK
                     YAVVTSVNRDDRKDGGAELFALTIRAIRDRVPGCRIEVLIPDFQGSYAAMDIVLDAAP
                     DVMNHNTETVPRLYRQVRLGARYERSLDILAYSKRARPEIPTKSGLMLGLGELKGEVV
                     PVMRDLRAHHVDILTLGQYLSPSPKHLPVIRFIPPDEFAEYRFLGEQLGFRHVEAGPL
                     VRSSYHAAEASARSTA"
     gene            complement(30482..31789)
                     /locus_tag="VLE22_17515"
     CDS             complement(30482..31789)
                     /locus_tag="VLE22_17515"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSB16257.1"
                     /translation="MTEQRWRKSREYLERARQSLAGGVSSPFRAKFPVPLYFADGCGP
                     RLTDVDGNEYIDYTLGWGPNILGYRHPALVEALRKQADGVHTFGAQHELEYLVSEKVQ
                     QLAPCAERVAFSSSGSEAVQLALRLARGFTGRNLILKFEGHYHGWLDSVLLSYHPRAD
                     QVGDYAAPNVVVESRGQVTNAAENVVVAPWNSVESVTRVFERRGGEIAGVIMEPVLCN
                     SGCLPPAPGYLEAIAELCRRNGSVLIFDEVITGFRMGAGGAQGHFGVKPDLATFGKAI
                     AGGMALSAVAGRQDILDTLFTGGVAFGGSFNGNPMVLAAAHATLEELSRDGGAALARA
                     NRTGAAVMEGIRDIAKRRGVPMTVCGFGAAFAVHFTEKAELRDYRDTLEDDREKLARF
                     AKALLSEGVYVLPDGRFYTSTVHTEREAGETLQAVDRALASAA"
     gene            31818..33020
                     /locus_tag="VLE22_17520"
     CDS             31818..33020
                     /locus_tag="VLE22_17520"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB16258.1"
                     /translation="MESPDQATRRGFLGSLGATAVLARQRSAAPVRVGVVGGGFGSSF
                     QWHLHPNAKVAAVCDIRPDRLQRLSEVYRCGTTLKSFRELLKQPELDAIAVFTPAPLH
                     VWMATEAMKAGKHVISAVPAGISIEECERLLECVKSTGMKYMMAETSYYRPEIMTCVE
                     WAREGRFGTIFYSEAEYHHEGLLPLMYDDRGFPTWRYGFPPMLYPTHSVGMIVPVTGE
                     RLAEVQAVGWGDGHEILQTNQYKNPFWNTTGFFKTSGGHSSRVSVFWHVAAGGTERAQ
                     FYGSRLSYIMERPEGSPNTVIRIARDGKTVIDANGYPEGDVAIEAFKQPDHFEKLPAP
                     LRVKTGHGNSHTFITHEFVSAILEDRQPAVNAWEAIAYTVPGIVAHQSALRGGEPMKI
                     KDYGKPPA"
     gene            complement(33047..34267)
                     /locus_tag="VLE22_17525"
     CDS             complement(33047..34267)
                     /locus_tag="VLE22_17525"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB16259.1"
                     /translation="MERRSFLRSVSMGAGAWLARTSAARAAAGPMKIKRIRYYADPNS
                     RPIYNQSSNIVTVETDQGITGIGEGGTKDTIEQCAAMLIGEDPGRIDHLWQMMYRGFF
                     YPAGREKLHALGALDLALWDIKGKALDTPVYELLGGLARDHIECYSTGFPKKGSLRET
                     ARACVESGFRAFRVGVADPSEGRPFNSREMVHRTVEMCREIREGVGKDGDWAIDYHTR
                     LDFADAVRLSTLIEDLEPYFVEDLVRSENPGVYRTLRPQVKVPIAVGEQFGDRWELNE
                     LVEQHLIDYSRVSVPNCGGITEFMKIAAMCETHYVGLVPHFTGTLATASLVHCCGVFS
                     GPVLMEMLGAGPREVPHLPQNFDFRNGKLWPNRRPGLGIEFDPKRLKMIAEVTERSRP
                     IPIYNRPDGSITNW"
     gene            34316..35521
                     /gene="tyrS"
                     /locus_tag="VLE22_17530"
     CDS             34316..35521
                     /gene="tyrS"
                     /locus_tag="VLE22_17530"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSB16260.1"
                     /translation="MGFLPVDEQFAYLRKGFVEVIREEELRERLTASLSTGRPLKVKV
                     GFDPTAPDLHLGHTVLIRKMKHFEDMGHTVVFVIGDGTARIGDPTGRNITRPPMTREE
                     ILANAETYKIQVFKILDPAKTEVRFNSDWLDQLGFESMIRLCATYTVARLLERDDFAK
                     RFKENTPISVHELLYPLAQAYDSVALHADAELGGTDQKFNLLVGRDIQRDFGQPSQIV
                     GTTPLLEGTDGVNKMSKSLGNYVGITEPPEVMFAKLMSISDELMYRYYELLTDVSLTD
                     IDRLRDRIRTGDLHPMQAKIDLASLIVTDFHSAGDAARAAEEFNRVVRRGEVPADLQT
                     VRIDGDVRRIDKVLAKAGLVASVSEATRKLKEGAVEIDGTKVEGLIQLASPGEYVVRV
                     GKNWRRVTL"
     gene            35525..36355
                     /locus_tag="VLE22_17535"
     CDS             35525..36355
                     /locus_tag="VLE22_17535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB16261.1"
                     /translation="MPPIEKIVPLETSHIPFVMRLNEAAGWNQTEKDWARVISVEPEG
                     CFALFADGVLASTATAVCFGRALAWIGMVLTDPAFRGRGYARRLMEHALEFLDRRGME
                     WIKLDATDMGRPLYLRLGFEDEAPVERWVLDNVPAMPRVDLPPYEPDPALDFEAFGAC
                     RSRMLELLSAEGAASIPGEGFAMARAGAKADYFGPCVCRSPQAARRFLEWFLSRSPGR
                     SAAWDILPTNVEAVRLAREFGFERRRQLVRQVRPGPAAGQPLPHNDAYVFAIAAFEYG
                     "
     gene            36361..37212
                     /gene="folP"
                     /locus_tag="VLE22_17540"
     CDS             36361..37212
                     /gene="folP"
                     /locus_tag="VLE22_17540"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSB16262.1"
                     /translation="MPRKRYEWKLKDRAIQLGDRTLIAGILNVTPDSFSDGGKYLDPD
                     RAFARALELEEEGADIIDIGAESTRPGSARISEADELRRLVPVLKRLKNKLMVPVSVD
                     TYKSGVAAKALELGAEIVNDPTGLTSDPQISRVVTLNDAGLILNHMRGTPETWAKLPP
                     LPDVMGTILHDLDASVHRARGAGIDRSRIVIDPGLGFGKRKDQNFEILAHLSQLARLD
                     IPVLIGPSRKSFLAQSTEQLTAFATASAVAACILGGAHIIRVHEVREMRAAADIADAI
                     LQSVAGE"
     gene            complement(37215..38504)
                     /locus_tag="VLE22_17545"
     CDS             complement(37215..38504)
                     /locus_tag="VLE22_17545"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB16263.1"
                     /translation="MQNYIRMLLAVAVMAAGAAGEERTLTLREAVDLALKQNPDLALA
                     RLAEGKAEQGVRLARDPFIPKVAAGSGLAYSNGIPLSVAGSTPSIFQAQAVQYVFNRR
                     QSHLISQARENARGATLDTAAKREEAAHRAATLYLEAERAAKVAQMARRQVESLTRVA
                     DVMRARVSEGRELPIEAKRADLRVAQARQRAEALDVERANAEGALTAALGLNADDRIR
                     VTVEERPAPQLPESEQAAVSAALDNSKEVRRLESALVAKGFEIKAERAGRLPQLDLVA
                     QYALLGKFNNYEDFFRKFQRHNGQLGVSFQIPLFAGPGVDARAAQAEGDAARLRIELQ
                     GTRNRISLDAQRLYREVRQAETAREVAKLDLDVAREQVSVLLAQMEEGRAPLRQVEEA
                     RFTEDEKWIAFVDSNYALETARLNLLKQTGELLAALR"
     gene            complement(38536..39738)
                     /locus_tag="VLE22_17550"
     CDS             complement(38536..39738)
                     /locus_tag="VLE22_17550"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB16264.1"
                     /translation="MHLGEALRFSLHALRANKLRTFLTSLGMVIGNASVILVVTISLT
                     SRDYILEQIRGIGSNIVYAYYETGGKSAAEVEADFVKLSDVDAVRQQLDSRIVAATGV
                     MTNYDRMLIEGREEDVKVIGTDEHYAAVRNLVLLSGRFLDWSDVSLREKVAMLTERLA
                     RRLYGSPQSAVGQVLKVHGLQFTVIGTFKERVESFGLSELSRETVVIPITVIRYFTPV
                     ERIDPMYVQVGRPEDVEAVTANVGEILRSRHRPGARYLVENLTAILDAAKSIALVLTL
                     VLILISAIALVISGIGIMNIMLVTVTERTREIGVRMAVGASRREVLRQFLIEAVMISL
                     GGGLLGILVGLALPFSVRLFSDVKIPISIASIVVAFVVSCVVGLVFGLLPANRAARLN
                     PTEALRYE"
     gene            complement(39746..40600)
                     /locus_tag="VLE22_17555"
     CDS             complement(39746..40600)
                     /locus_tag="VLE22_17555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB16265.1"
                     /translation="MRKLLTVLALVAGSALAETAALEQARSLYQKTRYAEALKVIESL
                     PQADGARWELAGRCRYMLDDFKGATEAFEKALAAEPKNSVYANWLGKAHGRRAETSSF
                     LTAPGQAKKARQAFEKAVALDPRNLEAVGDLFEYYLEAPGFLGGGAEKAAELAERSKD
                     LNPAEYHFFFAKLAEKRKDLKTAEAEYQRAIELAPGQVGRVIDLARFLAKQGRHEESD
                     AAFRQAEKIAPGAPKLLFAQASTYVRSKRNLGTARSLLERYLTLALTPDDPPRREAER
                     LLKEARTG"
     gene            complement(40806..42098)
                     /locus_tag="VLE22_17560"
     CDS             complement(40806..42098)
                     /locus_tag="VLE22_17560"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSB16266.1"
                     /translation="MNVRFSSRILRVLFALAAAIACLPAAGQGLPTAKPEEVGLSAVR
                     LERIGAAVQREIEAKRIAGAVTLVARKGRVAWLKAQGMADVESGKPMQTDNIFRICSM
                     TKPITSLAVMMLYEEGRFLLNDPVSKYLPEFKNPKVLVKPAAGEPYTIPAAREITIKH
                     LLNHTSGLVYHWNPDLGPRYRDAGVAHGLLQYDGTIAESVKRLAAQPLLFHPGDKYEY
                     SLSIDVLGRLVEVVSGKSLDEFFRTRIFEPLGMKDTHFYLPSEKVSRLASVYMRDEGK
                     AISRFPAKPIVEGPFEYSADYPYRGPKTLFSGGAGLNSTAADYGRFCQMMLNGGVLDG
                     KRLLSRKTVELMTHDQLGKVAPDLSFGLGFGVSGGKVPMDELGSPGLYGWGGFYYTVF
                     FIDPKEQLVGVFMAQLHPTGGLNLDGTFQVLASQAVVE"
     gene            42334..43668
                     /locus_tag="VLE22_17565"
     CDS             42334..43668
                     /locus_tag="VLE22_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSB16267.1"
                     /translation="MGKRRLIRRFVALAAVSLLLPLCSAQLRQVPEPAVGAAAASQDD
                     YIVTFQPGLSQAERAAAVVRAGANARFNFSIINAVAVRVPNANVLAAIQRDMRVVSIV
                     PDRPVHAFGKPTKPGGGSSGQVVPEGVKRVNTPVLGSSDGSGIAVAVLDTGIDLNHAD
                     LAIGSSRFDAFGGSCQDGNGHGTHVTGIIAALDNSIDVVGVAPNATPYCVKVLDDGGS
                     GSDSAVIAGLDWVYNRTDLQIRVVNMSLGRDKVDGDMALDSPMRAAIRQLYAKGIIVV
                     AAAGNEAAKEVIQKVPAGYPEVLAVASTTAKDGSNACRLLPNPIRKDTASFFTTDGSL
                     DSTSDGSIGVTISAPGEDQENVSRGCLISSVGILSLKAGGGTIRYSGTSMAAPHVTGI
                     VARVLQKELAVGPEGVRGYFQANASAKQTAPLDSPTSTYTFDGEREGVAVAP"
     gene            44231..44473
                     /locus_tag="VLE22_17570"
     CDS             44231..44473
                     /locus_tag="VLE22_17570"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HSB16268.1"
                     /translation="MNPNPAFDRITVEPGKCGGKPCIRGMRITVRRVLEILATYADRQ
                     EIFREYPFLEEEDLQQALRYAAASVDEELVDLNRVA"
     gene            44470..44838
                     /locus_tag="VLE22_17575"
     CDS             44470..44838
                     /locus_tag="VLE22_17575"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HSB16269.1"
                     /translation="MKLVLLDQGLAPRAATLLRSEGWEALHVSEVGLHRAEDLEILEF
                     ARQRGMVCVTLDHDFHTHLALSLSGSPSVIFVRVAGLDAYQQVVLIKAVWEVCGEAIA
                     EGAAVSTDGTTVRLHKLPLK"
     gene            45388..48510
                     /locus_tag="VLE22_17580"
     CDS             45388..48510
                     /locus_tag="VLE22_17580"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HSB16270.1"
                     /translation="MRSKVLIVLLIAIVTAAVAAAALAPSSFSTEAVNSYAELRRLFP
                     NPPASYSTAPFFVWNGEMTESEIDKHLADYHAQGIRAFFIHPRPGMITPYLSDRWFAL
                     CRYTVDKAKQLGMQAWLYDENSYPSGFAGGHVPAEMPESWNEGQGLVLHKLTQFPADP
                     GKYKIILKKAAGSFEDVTASASPEQGRQGDYLAFDLSFYEKSPWYGGFTYVDLIRPGV
                     TEKFIEVTMRGYERTLGPDLGKTVPGVFTDEPNIVPPADENCIRWTPDLFDQFKKRWG
                     YDLRPHLPSLFEQTGEWQRVRHNYYALLLDLFIGRWAKPWNRYADSKGIAWTGHYWEH
                     EWPNPRQGPDNMAMYAWSHVPGIDMLFNQFAENVAAQFGNIRSVKELASVANQVGARR
                     ALSETYGGAGWELRFEDMKRLGDWEYALGVNLMNQHLSFQTIVGARKYDYPQSFTYHE
                     PWWSHYRVLADYFARLSLALSAGEQVNRVLVIEPTSSAWMYASAGQADPRLMQLGETF
                     QAFVTRFEKLQGEYDLGCENIIRDRGKADGARFVVGRRAYDLVVLPPGTESLDAATVK
                     LLGEFLRGGGKVLSFVEPPARVDGAEDARVTQLASQFDSSWTRADSLDDPQARKMLLP
                     AGITDVAGQLLHQRRVLDGGELLFFANHSLEQPARATIKIAGKSLMRLNPFDGSTTAH
                     PARREGNSLVFSVELPPAGSLLVAALKEGPAPPAPSPAPVETPVAASGSLAVRRLAPN
                     VLQIDYCDVKLGGTVTEDLFTYNATDKVFKHYGLEANPWNTAVQYKTSILDMDRFPAD
                     SGFDAAFHFDVAAGVDASAFRAVVERPHLWKVTVNGKPVTARPDEWWLDRAFGVYDIG
                     AHVTAGRNTILLAARPMSIHHEIEPVYVLGEFALDAQPRGWKIVPPQSAAPGAWKEQG
                     LPFYSNAVSYTRIYTLKKGAFKVRLGKWHGTVAEVKVNGKPAGIIGWQPYELDVSRFV
                     RGGDNTVEVIVIGSLKNLLGPHHGKISRGFVSPWLFRNAPEHMPPGSGYDLDSYGLFG
                     DIELIESRPR"
     gene            48517..>48870
                     /locus_tag="VLE22_17585"
     CDS             48517..>48870
                     /locus_tag="VLE22_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16271.1"
                     /translation="MPGYHESDINRLLKTFRHQEADRVPHLEFWVTSKPVYEYVLGYP
                     LQYDVVDARVGGQSITPEDHVLFARKLGMDAVACNFSWRPNNVFQKAADGTEHYTGGT
                     VRTRDDFSRLEPPPAL"
BASE COUNT         8759 a        16707 c        14402 g         9002 t
ORIGIN      
        1 ccgtcggcgt ctgccggtcc acgtccaccg tcgccgtcga ttcgtacacc ggcgtcagcc
       61 gcgccgacac gatcaacgtc cccaccacgc tcatccccac aaacgccacg atcttccacc
      121 gctgccgctt caatatccat aaatagtgag acagggggac cgagggctcc tccgcctcga
      181 catccaccgg catcgcgggt ggtacatacg acggcgccga ataccgctcg atcacggccg
      241 gaagactacg gtcaagttct ggttcgcgca tagtcagttc tcttcgcgcc cgaaaggtgg
      301 cgacactgcc gccctaacga cgccagatca gaagcccgga ggcggtggcg gttccgaatc
      361 cggcgatctt ctcgagtgtg ttcacggcga tccgccggcc gctgttatcc gggatgtaga
      421 caagatcgtt cgcctggagc ggcacgtcgg gcgccttacg cttcaggatc tcgctcaacg
      481 cgacggggat ctcattccgc gcgcccgtcg agagttcacg gcggtagacg tacgcctgtt
      541 tggccgcgta aggagccaga ccctcggcca gcgcgaggac gtgcagcagc gtcgtttccg
      601 acgtgtcctg aacggggtac gctccgggcc tcttgacgtt gccgacgacg aagatcttcc
      661 ccacctccgg cacacggatt tcctcgcccc cggcgagctt caggtttagt tccggatcag
      721 cagcctctat cagcccccgc actgaaatcc gctggacaac cgtcgaagtc ttgccgtctt
      781 ctccgcgctg ggtccggctt accagaatct cttgccccgc atcctggctc agccccccgg
      841 cccgcgtcag cgcgtcgagt aaagtcatcg ggcccgtcgc ctggaacgtg accggatttc
      901 gcaccgctcc ggcgacgctg atcggccggc tgtggtactc ggcgatcgtc accgtcacca
      961 ccggctcgac gagaagctgt tcggcctcga gcgcctcggc gagggcggcc tccaactcgg
     1021 ccggcagcaa accgtcggcg cgcactcgcc gcttgagcat cggcaggcgc acaagcccct
     1081 ccgcgctgac ccgcaccgtg cgggtgaatt caggcgcgtc gtataccgag acggcgatca
     1141 ggtcgttcgg cccgatcttc tgagccggaa ggtttgccac gacggcctcc ggcacggacc
     1201 gcacttgcgc aacggcgatc ccacagagcg cgatcgaggc aaagcatacg gcaaacgcca
     1261 ggcggcaaca ccaccgccgg cgggcgaagt gagtcatgag atcaccttcc gttttgatct
     1321 tccagcagct tcagcaacct ctccacgcgt gactcgatct gggtcacttt ccgccccagg
     1381 cttgacaacc tggtttggag cggctcgtcg gcgggcctgt cgatcccagc cgaacggagg
     1441 acggcggaac gcgcaaaatc ggagaggctt cgcgcaccgc gactgacgta cgctatcttc
     1501 aggtcctcgt attcgtcttc actgagacga aaaatgacaa gacggtttct gggcctaagg
     1561 actggcattg ggcgatcggg aagcgatgcg gacgtttgga ggcaaagctt atgctatgtg
     1621 agtcccaaca tgggaaccca aaacacaaag gacacgtcgc gcggggaagg tgaatggggg
     1681 aggctgagaa tcggagcgcc accgcacgca ggttcaagcg ctcacttccg atgtttcaca
     1741 aagagtatac ggaaccttac cagactgtca agaagaaatt tcaggtgcca tggatcagag
     1801 gataaaatag cgtgtgttta cttgttcttg tgaacggatg tgaggatata gtggagattg
     1861 gtcgtgtaat gcagttgtac atcgcagccg gcatcggctt ggccgtgctg gttacattcg
     1921 cgtttttagc agcacggcgg ttagcggatg agcggcgcct cagagccatc tggaagtcgc
     1981 tcgaaggcga cggccggggt gcggcgttcc atccggatat ggtgaccgga cttcccgagc
     2041 ccgcgcgccg ctatctgctt cacgcgatca aacccggcac tccgctcgct tcctcggttg
     2101 cactgcgcat gaaaggcaag ctgcggccga aagccactgt cccgtggacc gagatgaagg
     2161 cgcgggagat catctgcgtg acgaaaggct tcctgtggaa ggccaaggtc aggcgcggcc
     2221 tgtctcgtgt cagcggagcg gaccactacg cggacgggca agctcgcgtg cgcttctggt
     2281 cgtggggcgt tttcgccgcc gtgcgcatgg aaggcgagga cacgacgaag tccgccatcg
     2341 gccggctggc gatcgaggcg gtgtggcttc cctcggcgtt gctgccgcaa tcaggggcga
     2401 cgtgggaggc ggtcgacaac cacgccgcca aggtcacgct caagatcgac ggccagccga
     2461 cgacgctggt agtgaacgtc gagcccgacg ggaagcttcg ccgatcgatc atttctcgct
     2521 ggggcggagt gggtgagagc ggcgaggcgg tgtggatgcc attcgaggtg gagtcgcacg
     2581 aagaacggga gttcggcggc tatacgatac cgtccttctg cacggccggc tggtgggtgc
     2641 gctccgatca cttcttcgaa ttcttccgcg ccaacgtcga gagcgccgag ttccgctgac
     2701 catggccgca ctcaggcgat aatagaacag aggcgcactc ctctatgcct ctgcctgggt
     2761 tcgatccgct catcgacgaa tggtttcgcg gccgtttcgc gaaggtcacc gaaccgcagc
     2821 ttcttggctg gcctgaaatc cgcgccgggc gggacgttct gatttccgcg cctaccggat
     2881 ccggcaagac gctggctgcc ttcctgatct gcctcgacga gctggtgaaa tcggcccgcg
     2941 gcggctcgct cccgaaccgt acggaaatcg tctacgtctc ccctctcaag gcgctcagca
     3001 acgacattca gaagaacctc gaagcaccgc ttgccgagat ctcggcgctg gcggacgcga
     3061 aagggttgcg cctggccccg atccgcaccg cggtgcgtac cggagacaca accgtccggg
     3121 aacggctgcg gatgctggag cagccgccgc acatcctggt gaccacgcca gagtcgctct
     3181 tcatactcct gacggcggac aagagccgta agattctaca gcacgcaaag gcggtgatcg
     3241 tcgatgagat ccacgccgtg gctggagaca aacgcggctc gcacctcgcc ctgaccctgg
     3301 cccggctcga agctctggta cgcgaggcaa gcggcggcac gttgcagcga atcggtctgt
     3361 ccgcaacggt gaagccgatc gaggaggtgg cgcggctcct gagttcctcg gcgggcattg
     3421 tcaacgtcgg acaccgccgc gagatgcacc ttgcggtcga gtccccgaag gacgagcttg
     3481 gagcggtcgc gacactgagc ttgtgggagg acatctacga ccggctggcg gaactgatcc
     3541 ggacacatcg caccacgctg gtcttcgtga acacacggcg cctttccgag cgcgtcaccc
     3601 accaccttca ggaacggttg ggcactgagg cggtgctcgc gcaccacggg agcctgtccc
     3661 ggcgactgcg gctggacgcg gagcagcggc tcaaaaacgg agagttgcgt gcggtggtgg
     3721 cgacggcgtc gctggagttg ggcatcgacg tgggtacgat cgacctcgtc tgtcagatcg
     3781 ggtcaccgcg atccctctcg gtggcctttc agcgcatcgg acgttccggg cactgggtcg
     3841 gcgcaatgcc gaagggccgt ctgttcgtca cgacccgtga tgacctgatc gagtgcgccg
     3901 cagtggtgcg ggccgtccgt cgtggggagc ttgaccggtt ggagattccc gaagcgccgc
     3961 tcgacgtact cgcccagcag atcgtcgcgg ctgccgcgtg cggcgagtgg cgcgaggacg
     4021 atctgttcga actggctcgc gggacctggc cgtaccggtc gctcgcacgc aaggatttcg
     4081 acgccgtggt cgagatgctc gcggagggaa tcgccacagt ccgcggccgg agcggtgcgt
     4141 atctacatca cgatcgcgtg aacggccgga tccgcggccg gcgtggcgcg cggctcgccg
     4201 ccatcacgtc cggtggagcg attcccgaca acgccaacta cctggtagtt gcggaaccgg
     4261 aaggcgccac cgtcggtacc gtcgacgaag acttcgctgt cgagagctcc gcgggcgacg
     4321 tcttcctgct cggtacggtg tcatggcgca tccgccgtgt cgagcagggc aaggtacggg
     4381 tggaggatgc gcatggggca gctcccaccg ttccattctg gcggggcgaa gcgccgggcc
     4441 ggacggccga gctttcaagc gaggtgtcgc aacttcgtga agaaattgca gcgcgtccga
     4501 aagaagaagc cgtcgagttt ctgctccacg agtgctcact cgaccgccgg ggcgcggagc
     4561 aatcggttga atacgtccac tccggccggg cggctctcgg cgcgcttccg gcgcaacgca
     4621 ccgtggttgc agagcgcttc ttcgatgaag cgggcggaat gcagctcgta atccacgctc
     4681 ccttcgggtc gagaatcaac cgcgcctggg gattggcgct gcggaaacgc ttctgccgat
     4741 ccttcaacat cgagcttcag gccgcggcca ccgataacgg catcgtcatc tcgctcagcg
     4801 agcaacattc gtttccgctg gagatcgtgt tcgtctttct gaagccggaa aacgttgcgt
     4861 acgtgttgac gcaggcgatg cttgccgcgc ccttgttcac cgcgcgctgg cgatggaacg
     4921 catcccgtgc tctcgcgatc ctgcggtttg ccggcggccg caaggtgcct ccaccgatcc
     4981 agcggatgcg cgccgaggac ctgatcgccg cggtcttccc cgaccaagcg gcctgcgcgg
     5041 agaaccttga cggcgacatc cgcattccgg atcatccgct ggtcaccgag accgttcgcg
     5101 actgcctcca tgaagcgatg gacgtcgacg gacttcgagc ggtgatcgcg gcgatggaga
     5161 gtggggagat ccgcacgttg gcgcgcgaca cgccggaacc gtctcccttc agtcatgaaa
     5221 tcctgaatac gaatccgtat gcatacctcg acgatgcgcc gctcgaggaa cgacgtgcgc
     5281 gcgccgtgca ggttcggcga acgattcgcg cggacgtgat ggatggcgtc ggggacctcg
     5341 acccagcggc catcgcggaa gttgcggagg actcgtggcc atcggtccgg gacgcagacg
     5401 agctccatga cgccctatta acgcttgtga ccgtgcctcc atcggcggaa tgggagccac
     5461 acttcggcgc gctcgtggcc gcgcaccggg ccactacgtt ggagacaggt ggcaggccgt
     5521 tctgggttgc ggctgaacga atcgatctgg cgcttgccgt tcactcggac ggcggtctcg
     5581 cgccgtggat tgagaccgtc cttccctcat cgccgattcc ggggacggtt gaggaatgtg
     5641 tcgccgcgat cctgcgcggc tggctcgaat cctgcggtcc cgtaacggcg ttcggacttt
     5701 ccgggcgcct ggcgatgccg ctcgatctag tcacgtcggg cctcctgcgt cttgaagcag
     5761 aagggcaggt tctgcgcggc cgcttcacgc cggacctccc gaacggagaa ctcgagtggt
     5821 gcaatcgccg cgttctcgcc cgtatccacc ggctcacgct cggcaagctc cgccgcgaga
     5881 tacagccggc ttcgacctcg gaattcatgc ggttcctctg ccggtggcag cacatcctgc
     5941 ctgggcgcca actgcacggg ctcgagggcg ctttccagat catccgacag ctacagggtt
     6001 acgagatccc cgccgcggcg tgggagtcat tcgtcttacc ccgccgcatc gcgaactaca
     6061 gcccggagct gctggaccgg ctctgcctct ccggagaggt gatgtggggc cggatctcgc
     6121 gccatcccgc cttcgacgac cccgaacctg gtcctcggcg tgtccggccg acccgcgtcg
     6181 ctcccgtggc aatcttcatg cgtgaggacg ccgcactctt ccttggaggt ttggagaaga
     6241 accacgcgga cacgccgctg tcgcatcccg cgcgtgagct tctcgccgag ctcgaactgc
     6301 gcggtgcctc ttttttcggg gaactgcaac gagcgaccgg ccggctgccg agcgaggtgg
     6361 aagacgggct ttgggaactg gtcgccgccg ggtttgttac cgccgacggt tttgagaatt
     6421 tgcgtgctct gctggatccc aagcggcggc gcggcgaggg acgaggccgt cacgcgcggc
     6481 cacggcacgc cgccggccgg tgggcgctcc ttcgcccgca catcccgtca cccgatgctg
     6541 aggaggctca cgcccggcag ttgcttcgcc gttggggcgt ggtcttccgc gatcttctcg
     6601 cgcgagaggc ttcggctccg gcgtggcgcg atctgctcgt tattctgcgc cgtatggaag
     6661 cgaaaggaga aattcgaggc ggccgtttcg tggacggcgt cttgggtgag caatttgctt
     6721 taccggaagc cgtggaattg cttcgcgccg ttcgccgcgc cgggaagagt ggcgagacgg
     6781 tcgagatttc gactgccgat cctctcaacc tcgccggtgt gatcctgcct ggcgcccgga
     6841 tcagcgccct tgccggcgga acgatcaact tgcgcgacgg agttccggat gccgtgtgtg
     6901 gggcctccag cgggggaggc ccggtcacgg cggttgtcca gtctacctaa gcgccacaga
     6961 cgggcgccgg caagtcaggg gcaaattacg gcttggcagt cgttccggac gtcgttccgg
     7021 aagtcgtgtt tttcgaatgc cgcttgtggt gttgggtgtg gtgcttcacg tgcttcttcc
     7081 ccgtagctgg cgccgtagtc gtctgactgt ctgatgctgg cggtggggtc tttgtctctt
     7141 ccgtttgcgc ggctaagcca agcggcagga gcaatgctgc cagaaacaac gacgctgtaa
     7201 gcttcttcat cctttttcgt tctcctttcg atgcatttcg catcgtccat gctggatttg
     7261 agatgccgcc tgcggccgtt ggatttctag cccccgtagg ggattccagc cactgaattc
     7321 gggggatgcg ggttcccgcc cagatccccc agtcggtacc gctacttgga tgtcgccacc
     7381 tgcaggttat tggtaacaga gaacactccc gggacccctt tggcgaggac gccggcgaga
     7441 ttcttgtccc caggagagtc ggcgaggccc tccagggtgg catttccgtt cttcacgatg
     7501 atccgaatcg ggctgataga gcggatcgcg tatcgctcca acgctgagct ccggtaaatc
     7561 gcccggtaca gggcgagccg taaccgatcg tcgttgggcg ataccggcag gacctcgatc
     7621 tggttgtcca ccgactcgac gccttcgatg tctttcacga cccgctcagc gctcgacttc
     7681 aacgtgggcc gtgtcacctg cccgatgaga gtaaccttgg agccatccac cttgaacgcg
     7741 atgaagtcaa acacgccata gtacggcaac atcaggattt cgtgccgtac ttcgtgtgag
     7801 attcgttcac ttgcggttga tgcggcccgg tttgccagac cgatcgatgg gagcgccacc
     7861 gctacgacaa gcgcgatgca cgcgaacttg agccagaagt gtttcatttt cgacctccat
     7921 gaaaatagat gcattacctt tcccgtaggg accatcagaa tcagcggcat cggagctttc
     7981 cgcccactcc aaagggccac ccaaactatc cgcgcgggcc ctgcccggtt ctttccccat
     8041 gccgcccggg cgcctgccgc aggggtacgt acaggactga cggcctgcca gctcccgact
     8101 gcggatacag gtccattagt tcgtagatgg tctcgggcat gtttgtcgtg attggtagtc
     8161 ccagttcacg ggccgcttcg acggtaatcg ggaagtcgtg cgtccagcgg ccctcgctta
     8221 acgcgaccgc taatgccccc gccttgtcct tgggcaggtg cttcagtaac acgtcgccga
     8281 caaaataagc aacctgaatc cgggctttgg cggcaacatc agccagcacc aacatctcgt
     8341 cgctgatttg ttcaggccgc ttagcctgga gaagtctcac gatcgacgcc gccggcatat
     8401 cgccgatctg cggatccact ggtcccagca cggcgttggg atccattacg atctcgtcag
     8461 ccgcgagggc gatcagagtg ccgccactca ttgcgtaatg tggcacaaag accgtcactt
     8521 tacccttatg ctccacgagg gcctttgcaa tctgctcggc tgcgagcagc aagccgccgg
     8581 gcgtgtgcag aaccatgtcg atcgcctgat cttgaggagt aagacgaatg gctctcagga
     8641 tggcctcaga gtcttcaatg tcaatcgaac tgctgaccgg cacaccgagt acgctgacgg
     8701 tgtcctgccg gtggatcatc gcgattacac gggactttcg ctgttgctgg aagcgatcaa
     8761 ggactctgga acggctcgcg gccagccggt atggaccgca tgcctgctgg atgagcagga
     8821 aggccagcag cagccagaag agtgttttca cagtaagtcg aactcggttc ccccaggatt
     8881 tctctgacac tgtcatcgct atcctcctga acttggcgca accacgccgg tccgcggcga
     8941 aagcgacgat aatggcaact gatgcgacat gagacgtgcc atccggaccg gtccgtggcg
     9001 gacccgacta atgatactgc cgctgacttg acgcgtcgag cggtatttcg tgcctcgatg
     9061 ctcgcgaccg ctggcggaat cctcgcacga ggggaccccg ctgcgcgtcc caacatcctc
     9121 tggatcacct gcgaggacac gagtccggac ttgggctgtt acggcgattc ttgggcgcgg
     9181 acgcctaacc tcgaccggct ggcaagccag ggagcacgct acaccaacgc attctccgtt
     9241 tacggcgtat gcgccccgag ccgctcctcg atcatcaccg gcatgtaccc ggcatccatc
     9301 ggcactcacc acatgcgctc ccagggcgtt cctcccccat atgtcaagtg cttcaccgag
     9361 tatctgcgtg cggcgggtta ctactgcacc aataactcca aaaccgacta caacttcccc
     9421 gcgccgctga cagcctggga cgaatcgggc aaccgcgcgc actggcggaa gcggcccaag
     9481 gaccagccat tcttctcggt gtttaacatc accaccaccc acgagagcca gatctggccc
     9541 gcaaacaacg cgaaggcgac cgccatcctg gcgccggagg agcggcacga tgccgcgcac
     9601 gcgaaggtcc cgccttacta cccggacacc ccgctgaccc ggtgcgactg ggccaactac
     9661 tacgacctcg tgaccgcgat ggacaaacag gccggcggcc tcctgcgcca actggaaaac
     9721 gacggcctcg cggagaacac gatcgtgttc ttctatggcg accacggccg cggcctcccg
     9781 cgcgccaagc gatgggccta cgaggcaagc ctgcacgtac cgctgatcgt ccgccggccc
     9841 ggaacgatcg ccccgggcgc cgtcaccgac cgcctcgtaa gcctcatgga cctcgggcca
     9901 acgttgctct ccatcgccgg tgtcgaaatt ccgaaataca tgcagggccg ggcgtttctc
     9961 ggcgacaggg cggctcagcc tcggaagtat gtcttcggcg cccgcgaccg catggacgaa
    10021 gcgtacgacc tcatccgcgc cgcgcgggac ggacggttcc attacatccg caacctccag
    10081 tcctgcaaac cgtacgccca gtacatcgac tacatggaga cgggccagac gatgaaggag
    10141 atgcggcggc tcaacaaagc aagccaactg caaggtcccc agcggctgtt tttcgttccg
    10201 gagaaggcgc ccgaggagtt gtacgacatc ccgagcgacc cgcactgtat ccacaatctt
    10261 gccgctgccc ccgcgcatca gaacacgctg aaacggatgc gggccgcggt cgatgagtgg
    10321 atgagcgaga tcaaggatct gggaagcgtg ccggaggatc agttgaagga acgcatgcgt
    10381 cccggcggta agtggtcggt gacagcggct ccgaaaatca cacccgatgg cggctcgttc
    10441 gccgcgccgg tcgcggtccg gctcgcctgt ccgactgagg gatcttcgat cgcctggacc
    10501 acagagcaga gcgagggttc gcgttggaac ctctatggcc gcgagattgc tctcgacagg
    10561 accgcgactc tgcgcgtcaa ggcttgccgc ctcggatggg aagacagtcc ggaggtgcgc
    10621 gcggaattca agataggatg aacgaatgcg tctcgtctta ttgaccacct ccgtgctcgc
    10681 aatctcagga tgctcgcaag cgcccgctcc cgctcccaag tctgccgggc agcccgccgc
    10741 tgaggtgaaa atatcgcagt tctatgcgaa caccggtgag gtggagcgag ggactccggt
    10801 cacgatctgt tacggcgttg agaacgcccg cgcggtccgc atcgagccgc cggtcgagac
    10861 ccttaaaccc gcggtaagtc gttgtttctc ggtcaccccg gaccatggcg ccacgtacac
    10921 cttgattgcc gaaggatccg acggcaagac cgtgaccgca acctttcgag tcaacgtcaa
    10981 aggcagaact gccgcccgcc cggagttagt ccagctcttc ggctccagtt cctcagagat
    11041 tcaggccggt cagacggcga cgttgtgcta taccgtcgac gatgcgacgt cactccgcat
    11101 cgaaccgggg gtgccgacga tcaaggatct tcacagcggt tgtatcctcg tgaggcccaa
    11161 cgaatccacc gtctacacgc tgaccgcggt cgcggcggat ggccggaccg agagccggaa
    11221 actcacgatt accttaaagt aagcgtcccg ccggacatca cgatttcgga ctgacgcacg
    11281 gaagggtggg cgtcccatcg gcggaacggt cgtcccctgt gcgatcgcgc cagcgctcca
    11341 actctttccg catctcgtcc gcctgctgcc ggtgcgcagg atcgctgtat aggttgcggc
    11401 gctcctgcgg atcgttctgc aggtcgaaca gttcgtactc ctgcggctcc tggatgtaat
    11461 ggatgaactt ccatcgcttg gtccgcactc ccctgtgctt tcccgcgcag tgatagccgg
    11521 ggtactcgaa gtactcgtag agccacgagt cccgccacgg cgtctgccgg ccgtcgagaa
    11581 tcggccgcca gctttcccca tgcaccggca gggcggcagg ccgtttcacg ccgcaatagt
    11641 cgaggatcgt gtagtggatg tcgttgttca gcaccatatg ctcgcggtcc acctgccccg
    11701 ccctgattgc tgccggatac cgcacgagca tcggcacgcg gatggagggc tcatacatca
    11761 gccgcttgtc gaacatgccg tactcgccga ggaagaaacc gttgtcaccc gtgtaaatga
    11821 ccagcgtgtt ctcggcgagg ccttccttgt ccagatagtc gagcacccgg ccgaggttct
    11881 cgtcaacgcc gagcagcgtt cggtagtagt tcttcaggaa gagctggaag ttgcggcgct
    11941 tgcgctcctt cgggggcagg ctggctggaa cgccgcggtt ttggaagtcg atcatgtcgc
    12001 gaatctgcat gtccgagttc cgcaccgcgg cgggccggcc gctcaggtca acatcgaacg
    12061 ttggcggctc tggaatggtg atgccgtcgt acatcttctc gaaccgccgc gccggaatcc
    12121 aggtccggtg tggcgccttg aactggtaga gcaggcagaa gggcgtgtct tttgggcggc
    12181 tcttcagagt ctccagcgcc tgatcgccga tgacgtcctc cacgtgcccg cgaaactgca
    12241 tgcggctccc cttggagatc atcaccgggt cgacgtagac gccctgcccc ggcaagatca
    12301 gccagtggtc gaatccggcc ggttcggacg cgatgtgcca cttgccgacc agcgcggtgt
    12361 agtaccccgc ctcctgtagc agcatcggaa aattgaccgt cccgacgcgg aaatagtgcg
    12421 acccgctttc gttggtggtc acgccgtggg tgtgcgagta gaggcccgtg aggatcgtcc
    12481 cgcggctcgg cgagcagagc gaattggtga cgaacgcttc gctaaaacga acgccgccct
    12541 ccgccaaccg gtccatgttc ggcgtccgca gaatccggtt accggcgcag ctcatggcgt
    12601 cccagcgctg gtcgtcggtc atgacgaaca acacattcgg acgcttgatc tgcggctttg
    12661 cgatgttcgg gaatccggcg gccgcggcgc cacccaaggt ccggagcgcg tctctgcgtg
    12721 tcatgcagcg attatcgctc tacaggccca ggaactggcg catcaattcc tccagcgcgg
    12781 gaacgcacag aatacaattt ccgccgtaga tgctcgaccc gccatcgaaa ttgaagaacc
    12841 tggcgcccgc ctcctcggcg atgatcttca ggggcgcgag gtcccacggc ttcgcgctcg
    12901 gttcgatcca ggcttcggcc cgccctcccg cgaccatcat cgcgtcctgg catccaccca
    12961 tgctgcgaac ggcccagaac tgcttcgtga attccagcag ccggcctgca aatggaagat
    13021 ctgcgatgtc gttgatccca ttcacgcaga gcaccgattg cgaaggcgat gtgatctccg
    13081 acacccggac tctgtcatcg tttcggaggg cgcccgcgcc gcgagacgcg gaatacaact
    13141 ccttctgcgc cggctggtgg catacgccgg cgacgacctc gctatccgct tcgagagcga
    13201 tcagcatgcc ccatgtgtcg ctgccccgca cgaagtcgcg ggtgccgtcg acgggatcga
    13261 tgatccaacg gcggcccgac cggctttctt ttagcgcgcc ttcctcgccc aaaagtccgt
    13321 cgtcgggaaa cgcctcgagc agaatccgcg agatcagttt ttcgcactcg cggtccgcca
    13381 ccgtaacggg cgactcgtcc gccttcgcct ccgcttctac cccttgcgcc tgatagcgca
    13441 gcgcgatctc gcccgcctgc gtagcggccg cccgcgcaac ttcgagctct ttttcccaca
    13501 ccatccggcc cattctaacc ggcggcgtgg cgccggggtg catgctagga ttttcgattg
    13561 gggcaccgga cggcacttac cgcgctggaa gaggcagcct cgcagttcgg caagctcgcc
    13621 gctctctgcc agcctgccgg ggaaggaaaa caccgggtct attcctggat cgagtaccgg
    13681 gacatcgcgc gcgagattgc cgccggcctg cgctctctcg gtatcggcaa gggcgacatc
    13741 gtcgccttgg actccgaaac ccgcgccgag ttctacttgg ctgatcttgg cattctggca
    13801 aacggttcca tcgccgcggc gatgtacggc agttaccccg ccgcgatcct cgttcacacc
    13861 ctgcgatcct gcggtgcgaa ggccgtattc gtcgaggatc ccgatacctt gcgcgtgctc
    13921 caggccgccg gcgacccccc tctcgcggtc gactggatcc tcctcaccgg aaccgccgag
    13981 agcgctctct ccctggagga tttgcgcgag cgtggccgtc gctctatcga gtccgatcct
    14041 ggcctgcccg cccgcatcgc gagcgagctt cacccttcgg atccggccat cctgtacctg
    14101 acctcgggcg cgaccggcga gcccaaaatg gcgctggctt cgcacgccgc gcttctggcg
    14161 aacatggaga tggggccggc cgtgctgggc ttgacgccgg aggattccac catcgccttc
    14221 ctgccctccg ctcacatcgc gcaacgcgtt gtcgttgaac tgctgccgat ttgttgcggc
    14281 atgcccgtct ggttcgtcga gagcctagcc aagctcccgc cggcgcttcg ctcggtccgt
    14341 ccgacgatct tcctggcgcc tcctcgattc tgggagcgcg tctacaccag cgtgtgtacc
    14401 gaaattcaga aacgctcgcc ggttgttcgc aagctgttct atggagcgct gggactgggc
    14461 atagacgtgc accggcgtga gcgcgagggg aagtccgttc cgcgctggat tcgaaacacc
    14521 ctgaaactgg cggaccggct tgtgttccgc aaactgcggg cgcggttcgg cgaccgtctc
    14581 cgattgcctg tctccggcgc agcgccgctc ggcaaggaac tcgccgagtt cttcgaggta
    14641 atcggaatgc cgatcgtcga aggatacggc ctcaccgagg gcggcatctt gaccctcaac
    14701 ccgcttgacc ggccacggcc gggcagcatc ggaaagccct tgcccggcgt ggagattcgc
    14761 cttgccccgg acggagaact gctcgttcga gcgccctgtc tctttaccgg ctacttcaac
    14821 gatccgaagg ccacctcgga ggttctccgc gacggctggc tgtacaccgg cgataccgcc
    14881 gaaatcgacg ccgccgggtt catctacatc accggccgca agaaggaagt catcatctcc
    14941 tcaaacggca agaagattta cccggtgcgt atcgaaggct tgttcaagct cgaaccgatc
    15001 atcaaccacg tcgtcccgct tggcgacggg gagacattcg tcgcggcttt gctgacggtc
    15061 aatgcaggtg tcgcggagac gttgaagggg gcggagcacc tacgcggccg gccaatgtcc
    15121 gagatcgccg ccgctccccc cgtcgtcgcg gaagtcaaac gagcgttggc ccgcgtcaac
    15181 gcgcagttgg cgccgtttga acagattcgc cgataccgta tcctcgatcg cgagctgacg
    15241 atccaggacg gcgagctgac cgccacactg aagatccgcc gaagccgcgt tctgtcgaat
    15301 taccgggctg caatcgaaga actgtactca ggacgggaga actgatagcc gagagctatt
    15361 ccgcgagtcc cgccacgagg atttctcgcg tatcctcatc aatccggtcg agccggatct
    15421 cgatacggtc cgtgggccac agctccggaa accgctcgcc ccactcgatc agtacaacgc
    15481 tctcccgctc gaggagttct tcaagcccga gcgacgccgc ttcgcgcgga cgttcgatgc
    15541 ggtacagatc gacgtggtat acctttctat cctcgccgta ctcgtgaatg agcgtaaacg
    15601 tcgggctgga cacttcctcc gggtccgcgg cgcccagccc cttcacgatt cccttggcga
    15661 gcgttgtctt gccggcgccg aggttgccga tcagcaggat cgtagcgcgg gcaggcaatc
    15721 gggcggccag ggcctccccg aaagcgactg tctcctcctc agagtgcgtc cgatacgccc
    15781 gcgcactcat ccatcgcctc cgggagaaac tccagcatgt cggtagcgat caacgctttt
    15841 tcgccgaacg cggcggcgcc cagctccccg cagcgcccgt gcaggaaaac ggcggctgcg
    15901 accgcggcat tctcgcagtt cgggaactgc gccagcagcc cggcgatcag cccggtcaga
    15961 atgtcgccgc tgccgccggt ggccatcgcc ggtgtgcctg tggggttgat ccaaacgttc
    16021 ccgtccggaa acgcaagcag cgtgcgttga cctttaagca cgagcgtcgc cttccgtgac
    16081 accgcgaacg cacgagcagc gccaacccga tcgctctgca cctccgccgt gctcgacccc
    16141 gccagccgcg ccatctcacc cgggtgcgga gtcaacacca gcccgcgccc tccgaactcg
    16201 acgccggcaa gggcattgag accgtcggcg tcaatcacca gcggaacctc gacctcttca
    16261 accagccgct gtaccacggc tacggtctcc ggatgggtgc caagacccgg cccgagtgcc
    16321 accacctcct tgccgcgcac gaggcctgcg agtcgctcgt aatcgaacgc gcggagtgag
    16381 atcgctcccg tctcggtttc cggcaagggc tccgtcatta actccggtgc gtgcgcggcg
    16441 atcacaggaa tcgccgattc ggccgaagcg accgtgacca ggcccgctcc cgcgcgaagc
    16501 gccgccagcc cggccattgc cgccgcgcct gtcttgccgc gtgatcctcc gatgacgaga
    16561 acgtgcccga aatcgccctt gtgcgccccc ggcgctcgcg gtccgaggag atcctggaaa
    16621 agtagtggct ccaccagcga cagaaaaatc gatgcgtcgt tctcatatag ctccggcggg
    16681 ctgccgatcg gagaaactcg cagttcgccc acgtagtcgc aggccggagg cagcacctgc
    16741 gccgccttcg gagcggtgaa ggtgatcgtg tgccccgcct tcacggcgtc ccccggaacc
    16801 gtgccactgt cgctgtccag ccctgacgga aggtctagag ccacgatcat cgcatgcggg
    16861 aagccggtat tgatctcgcg aatgagttcg agcatccggc cggccgcggc gcccttcagc
    16921 cctgtcccga gcagcgcgtc caccacgatc gtggcttcgc gcatccgcgg cgtgatctcc
    16981 atcgccaccg gacaaccgca aacccgaagc atgcgaaagt tctccgcggc gtccccgcgc
    17041 agttcttcgg gttcgctcgc gagcactaca tccagtgagc gcggccggaa acgcgtatgg
    17101 atctgccgcc cgacgacaaa cccatcgccg ccgttattac ctttgccgca cagaatcacg
    17161 atccggtgat tcgccagcgg agcgaaggtt tcccgaagaa actccacgac gcggtgcccc
    17221 gcgttctcca tcagaatcaa gccggggatg cccaactcca tcgtgcgccg gtccactgcg
    17281 cgcatctgcg ctgctgtcag gaccttcatg actgcgcccc cgccatcagc acctccagcc
    17341 ggcgcaaccc cggcggctcg cccatcacga tcaggaagtc gcctccattg atctccgctt
    17401 ctgccggcgg gttgaacagc atgcttccgt ctgcccggcg gatagccagc acaatcaccc
    17461 cgacgtcacg gcgaatctgc atctgcttca gggtcttgga ggcgaattcg ctctgctccg
    17521 cgaccctcac ctgttcgatg gcgacatcaa gcccgatgct cctgctggtg aaatcgagga
    17581 accggaagac atgcgggcgg agaagcgcct gcgccagttg gtacccggca ttcgagtacg
    17641 gcgcgaagac tatgtccgcg cccgcgcggc ggagtttctc ctccgcttcc tcctcgtcca
    17701 cccgtgccgc gacgttcagc atcgggttca gatttcgggc ggaaagaatg acaaacaggt
    17761 tgtcagcgtc cgtcgccagc gcagcgacga gcccgcgggc gcgcacgatg cccacatccc
    17821 gcaacgtttg gtcgcgagtc gagtccgcct gtactgccag catcccggcg cggatcgcgg
    17881 attcaacccg ctcagggttc ctatctatca ccacgaaagg cgccccggac tgattcagtt
    17941 ccagcgaggc gtttcgtcct actcgcccga aaccgcacac aatatagtga ttctcgagtt
    18001 tgtcgatcat gcgcttgacc cgccgcttcc cgaagaactc cgccagttcc agctcgatga
    18061 tggtctgggt catcattccg atggcgaaga acatcgtcgt gacgccaaaa aatatcatga
    18121 cggagttgaa gacccgtcct tgcgtgctta aaacgtgaac ctcccggtag ccgaccgtcg
    18181 tcatcgtgat cagcgccata tagaaggcgt cgaacggagg atagtcttca atgacgatgt
    18241 agcctaccgt ccccaccagg agggtgcaga ggactaccag cccgacgtat cccagccgcc
    18301 gcgtcacccg gctcattgtc ctctcacttc ctcttcgcga gcagcagcgt catgtcgtct
    18361 tgcaactcgg gtgaattcgt ccattcgtgc acggccgcga tcaccgacgt gatgatatcg
    18421 gcagagcttc tccatacatt cttgagtagc acgtcgatca gaccctgctc gccgaacatt
    18481 tcgccgtatt cgttctccgg ttccgtgatg ccgtccgtga aacagaccag caggtcgccg
    18541 ctccacagtt gaatgcggct ctcgccatac tccgcgaacg gaaatgctcc caccaccatg
    18601 ccgttcgtct ccaggcgatg aacctcgccg tcccggatca cgatcggggg aggatgtccc
    18661 gcgttcgtgt acgtaagttc gcccttccga tcgtcgtaga tgccgaagta gaacgttgcg
    18721 aacttctcag cggaggtgaa gctgtagagc tgctggttca gttgagatac gagcatggag
    18781 gtagggattt cggcatggcc gtttccaccg cccggcgcgg ccgctgccgc cgccgtcgcc
    18841 tccaagcaag cccggatctg tgtgcgcaga gaggattgta gcgtcgccat cagcagagcc
    18901 gcggaaatcc ctttgccggc cacgtctcct atcgccagcg ccactttcga ctcgagtagc
    18961 ggaaggtaat cgtagtagtc gcccgaaact gtccgcgccg gatggcagag cgcagtcagc
    19021 tcgatccctg gcatcgacgg aaccgtccgc ggatagagct ggttctggac ctccctggcg
    19081 atctcaagtt cggattgaag ccgctccttc tccttctcca ctcgcacgag gcgttcgaga
    19141 ttctcagtca tgcggttgaa cgaatggctg aggtcggcga gttgatcgct gcctcgcacg
    19201 tcgatccggt gggagaagtc accgtgcatt accctctgcg tgccctcgta caggttgtgc
    19261 accgcctcgg tgatcttgcc cgtcatcgag acgccgataa tcagcgacac cagttccact
    19321 accagaaaca ggatggccac aatcagaaag gccgtattca ggtcgtcctg gcccgagccc
    19381 aatctctggc tgaagatggt tcgcacaacg gcggagacgc gggtctgcac gtccaaagcg
    19441 gcgatcgtcg tgtttccggg cgattcccac atggcgaccg gcacccaggc gaaccacgac
    19501 acctcgatgt cgaagcggtt tactgctggg gggacgccgg acgctgccgg cggggcctcg
    19561 cccacgttcg ccttttcgct gcgggtggac gctcgcctgg tcccctccaa aaccgacctg
    19621 gagatgagcc gaacatggcc aaggctcggg acgaggccat ccaaaaaatc gcggtccagc
    19681 cgggtcagga gggttccttg gatggcgcgg gactccgagt gcgcccacag atagaatgtg
    19741 ccgcctcgcg aaagaaatcc gcttttctcg ccccagccgt gcggagggga ctccagcttc
    19801 gattcttccg gataccgcca gacacttccg ccttcgcgaa tcagcacttc gaggccggga
    19861 aacctcttcg ccaggtacgg ggccatccag cggatgcgat cctcccgttc aggggcgggc
    19921 gtggcaatca ggccgatggt gggaccgacc agagacgagc tgtgccgctc cagttcggtg
    19981 atcaccaggt agagcgccat ctggcccgtc agcaaataag cgccgatgcc aacgagcacc
    20041 agaatcacgg ccacaggtac gacggcgata aagagatagg caacgatcag ccggttccgc
    20101 aaccgccaga tcgttttgcg catccagatt cgcaccagcc gcactgcggc aacaagaccg
    20161 ctggctgtga gtccgagttg aaccagaagg atcagcgatc ctgcgggcga agcaaacctg
    20221 aggatgagat acagcaccag cagcgcaaga aacgactttt caggccagcg gagcttgcgg
    20281 tagaacgatt tcaaggggct ggagaccggc atgccaaagt tcgcgcctcg ccgcgggcgg
    20341 cgtcaaccgt caaggcggta ttgtaacatc gggccacggc gagcaccggc ggcgccggtc
    20401 acggattgaa gtcgatcctt tcgatcagcc ggccgtcaag cgccttcgct tccagccgcg
    20461 ccttgctgcc ttccacctca atgacgacga aatgctcgat cttctctacg gtctgcgtga
    20521 ctcccggcac cggggcgtcc acctcgtata aaggcgcccc gccgcctcct gtgacgatgt
    20581 agtggacgcc gttcctgacg tggtgctgat agttgtggtc gtgaccattg aacactgccg
    20641 tcactttgta cttctcgaac agaggcatcc attcctggac gggatgcttt cctccctgcc
    20701 ggcgcttcac cgccgtgatt ggcgggtgat ggaagactac gaaccggagg tcggcttccc
    20761 ggctcttctc gagttccgcc tccaaccact ggctctgctc ctcccagaac gccttccgct
    20821 cttcttcgtt gcttgctgtg ttgcccaggt cgctgtttag taagacaaag tatgcatttc
    20881 cccatcggaa cgagtagtac ggcttctcga tgtcgaagaa ttcgtaaaat ctcggatcgt
    20941 tgcgctcatg gttccccagc gcgggaaaga acgcggtttt ccttagcaac tctcgctcga
    21001 tcccgaagaa cacaggccac tgctcggtct tcctgccgtc cgctaccaga tcacccgtgt
    21061 gcagaacaaa gtccggttcc gacttcgcca gcgcgtccac gacgttctga tggacctcgt
    21121 ggcgggatcg cgtgtcgccg aagaccgcga accggaaagg cgaggctccg tcaggcgccg
    21181 acttaaagta tcccttgcct tcgtcctgac ccgaaacatc gtagtgatac gtcttgccgg
    21241 gggtcaaacc ggaaatcgtg agtttctgcg cccgcagcac cacctcgccg ccttcggtga
    21301 actccagcac ccaagctacc gtggcgcgat cgggtcccac gcgcaccacg tacggcccgc
    21361 ccaccatttt cccggccgct tgcgcgtacg tccctacggc cgccaatgtc agcaacgttg
    21421 cgggaagcag aactttgtga aacatccatc accgcctctt caggaactct gaaacatcga
    21481 aataccggcg atccccgccg caccggcgtc caggcacagc gcggcattcc cggcgttcat
    21541 accgccgagc gcgtacacgg ggattcgcaa tccgcggcat gcctcccgca atcgctccag
    21601 cccttgcggc ggcccgtaca ccgccttgga tggcgtggaa aagaccgggc cgaacacggc
    21661 gaagtccgcg ccttcttcgc cagcgagccg cacttcgtcc gccgaatggc acgaaacgcc
    21721 gatcgtaaat cccggcggcg cgatcgcgcg gacccgcgcc ggagcaacgg agtccgacgg
    21781 caggtggacc cctgcggcgc cgcacaccat ggccacgtcg gtccgggagt tcaccagtat
    21841 ccttgtcccg tggggattcg gcagcgatag caccgctttg acaaggtcga tcagatcgga
    21901 tgtgcccaga tccttctccc tgatctggat catctcgaca ccgttcgcaa gtgcgcgccg
    21961 gatcgcaccg agcaagggcg caaccccgcc cagaggcttg cggtcggtga tgtaatagcg
    22021 ccgcattgtt acagcggctc gtagtagcgc tcgcccgcgc acgccttgca aagcgttttg
    22081 ccgtcgcgca cgatctctcg cttgaagttg atgccttcgc cgcactcggc gcacaccgtc
    22141 cggggagcct tgtagccggg cagttcctcc ggtcccagcg ccacccgcac ccatgtcgtg
    22201 gcgaagagtt cctcgtccgg cagttcgcgg tacgctttca tctgctggag attccggttc
    22261 gcgatctccg gataccgttc ggccgcccgt gccttggagg attcgagcgc aacgagcctg
    22321 accgcccggt tctcctgaag atcgcaaaac gtcgccgcca ccttgccaaa gtcccgaaac
    22381 ttcagcgcgc gcttgccgag ccggcagccg gtcaccacgc cgatggcgtc cgtcgcgcag
    22441 cgatcgatct cgacgaacgt caccagccgc tttcgatctt ttcccgtggg atcctcgatg
    22501 cccagcagcc tcagcccgta gacggccatc cgcagaccca gaatctggcc ggcgcacatg
    22561 tggccgtgcg ccatttcagc catggcctcg tattcccgaa aatccctcat cttgcccaac
    22621 acccagctca gaggatgcgc agatgctgcg cggggaactc gatcacccac tctctattat
    22681 gcttgtgctt atcgaattcc gcccgggtca cctcggcaaa gatctcaccc gtgaactcca
    22741 gccggaccgc gtgcgggcgc tcggtgtagc gcaccaattc cgtcggtacc tgattcggcc
    22801 cgggcttgcc gtcccaagga gtggccgcca gctcctcggg ccgcacacag agtatcaccc
    22861 ggtctccctt gagcctgccc ggaaaatacg gcccggtcag gtcgcggtcc tgaaagcgca
    22921 accggctcgt tcgctgcacg ggatcgagtg cgaggacttc caccggcagc aggttgaacg
    22981 tcccgagcag ccgggccacc tcgacgttcg cgggccggtc gaaaaccgcg cgcggcgccc
    23041 cgcgctgcac catgcgtcct tcgtgcagta cgagcatctc gtcgccgagt gcgaaacact
    23101 cctccaggtc gtgcgtcacc aaaagaatcg gactccggaa ctcggagcgc aactgccgca
    23161 gcacgtcgta cagttccgtt cgcaaaggcg cgtcaagccc gcgcgccggc tcgtccagca
    23221 gcaatgcctt cggcgctccg atcatcgccc gcgcgatcga gcaccgttgc ttttgcccgc
    23281 cggacacttc gtgcggccgc cgtccggcta cctccgccag gtgaaactgc tcgagcattt
    23341 cgttcacctt gcggtggcgt tccaggcgcg gccgccgctg ctttgaagcg cagaccgcgg
    23401 cgaactccag gttctgccgc agagtcatgt gcgggaacaa agcatagttc tgaaacacgt
    23461 aaccgcagcg ccggacctgc ggcggcaggt tcactccgga ggccccgtcg aacagaatgt
    23521 cgtcatcaag caggatgcgc ccttcgtcgg gcttgacgaa tccggctatg gcctcgagcg
    23581 tcatcgtctt cccggatccg cttggcccaa aaagcaccgt gatgccgctt gccgcgcgga
    23641 actccacgtc cagtgagaac tccgccgaat ccgggccggc gggaaatcgc ttgcggatcc
    23701 ttgcgttgat catgcggcct gcttcggcga cagccggttc gccaggaaga gtatgaacat
    23761 cgccgccgcc gagacgatga gcaccagtac tcgcgccatc gccccgtttc ccgcctcaac
    23821 agcgtcgtat atcgccaccg acatggtctg cgtccgcccc ggaatgttgc cggcgatcat
    23881 gatcgttatc ccgaagtcgc ccagcgaacg ggcgaacgcc agcaccgccg ccgccaggat
    23941 cgatcggttc gccagcggca gcgtcacctg aaagaagacc cgccattcgg acgcgcccag
    24001 cgtccgcgcc gcccgctcgt agctcttgtc aacgctctcc agtgcggctc gcgccgactt
    24061 caccagcagt ggcgtcgcgt gaatcagggc cgccaccacc gccgcctgcc acgtgaaaac
    24121 cagcggagcc ccgaacagcc actcgtaaaa ctttccgaaa gggctcgccc gcccgagtag
    24181 gaccagcaga tagtagccca gcaccgtggg cggcaacacg agcggcagcg tcaccgcggc
    24241 gtccagaagt tccttccccc ggaattcccg gttcgccagc atgtacgcca gccatagccc
    24301 gatcaccaga gccaccagcg tggctagcac cgccacacgc aggctcagcc acagcggaaa
    24361 ccaatcgatc tccatggaag acttgattat acccgccgcc gtcacgccgg ccagcgctgt
    24421 attctaaact tcgggataaa ccgtgatacc tgatactttc ctgcacacca cgctcggccg
    24481 aaccggcttg ccggtgttcc gcctcggcct ctccgcgagc tatcgccccg gaaaacgcgc
    24541 cgtccaccgc gccctcgacg aaggcgtcaa ctacttcttt tgctacggct tcgacacgca
    24601 catgacgggt gtgttgcgtg aagttcttcc ctccaaccgc gagaagtacg tcgtcgccac
    24661 tggggcatac aactacatct ggacatacca gaatatccgc cggacgctcg agaaaaggct
    24721 ccgccggctc aacacggact acatcgacgt cttccacctt ctcggcgtca ccaaacgccc
    24781 tcagttcaac gaccgggttc gcgacgagct tttcgcgctt cgagaagacc cgcgggttcg
    24841 agccatcagc atctcctgtc acgatcgtaa gttcgccgga gaactggcgg cgcaaggtct
    24901 tctcgacgcc ctgatgatcc gctacaacgc cgcccaccgc ggggcggaaa gcgatatctt
    24961 cccgcacctc gccgcccaca accccggtgt cgtgagctac accgcgacgc gctggaccta
    25021 cctgctccgc cggccgcacg gctggccgcg ggacgggcgg gttcccacag ccggcatgtg
    25081 ctaccgcttc gttctatcaa atcctcacgt gcacgtgtgc ctcaacgcac catcgaacct
    25141 aaagcacttc gaggacaacc ttgcctccgt ccgccaaggc ccgctcgacg aagacgacat
    25201 gcgcttcatg cacgagttcg gcgacgccgt ttaccgcagg aacaagtggt tctggtgacg
    25261 catccgaccg gccattccgg cgttacagta gtgtcatgac cggacctatc cgggctccgc
    25321 gcggccgcgc tcttcgttgc aagggatgga aacaggaagc ggcgctgcga atgctaatga
    25381 acaaccttga cacggacgtg gcagagcgcc ccggcgatct cattgtctac ggcggggccg
    25441 gcaaagcggc gcgtaactgg gagtgctacc acgccatcgt gcgtgaactc gaggccctcg
    25501 gagacgacga cacgctgctc gtccagtccg gcaagcccgt cggtgtgttc cgcacccacg
    25561 aggaagcgcc gcgcgttctg atcgcgaacg ccaatctcgt tggccactgg tcgaattggg
    25621 accatttccg cgccctcgag cagctcgggc tgatcatgta cggccagatg acagccggaa
    25681 gctggatcta catcgggtcg cagggcattg tacagggcac gtacgagacc ttcgccgccg
    25741 ccggccgtaa gcactttgaa ggctcgctcg ccggcaagtt cgtggtctcg ggcgggatgg
    25801 gcggaatggg tggtgcacaa ccgctcgcgg ccgtcatgaa cggcgccgct ttcctcggca
    25861 tcgacgccga tcccgagcgc atcaaacgcc gcctccgaac cggctactgc gacatcatgg
    25921 tgaccgatct tgacgaggcg gtccgcatcc ttcggcatgc tgtttacgag aaacgcgcca
    25981 cctccgtcgg tctcgtcgga aactgtgccg aggtgcttcc cacaatggtc cgccagggga
    26041 tcgttcccga cctcctcacg gaccagacca gcgcgcacga ccctttgaac ggctatatcc
    26101 ccgccggcct cgcggttgac caggccgcct cgctccgcgc ctcggacccg caggagtatc
    26161 tccgccgctc catggactcc atcgcgattc acgttcgcgc catgctcgac cttaagcgcc
    26221 tcggctccgt cgtcttcgac tacggcaaca acatccgccg catggccgcg gatcaaggcg
    26281 tcggcgatgc gttcaccatc ccaggtttcg tacccgaata catccggccc atgttcatgg
    26341 aaggccgcgg accgttccgc tgggtcgccc tttccggcga agcatctgac atcgaggcaa
    26401 cggacgacct cgtcctccgc ctcttcccct ccaatgaaac gctgggacga tggatttcac
    26461 tagctgggaa gcgggttcga tttcaaggcc tgccggcgcg gatctgctgg ctcggttatg
    26521 gagaacgcgc cgaattcgct ctggagatga atcgcatggt tgccacaggc aagctgcagg
    26581 ccccagtcgt gatcggccgc gaccacctcg actgcggctc cgtcgcatcg ccgtatcgag
    26641 agaccgaagg catgcgggac gggagcgatg ccgtggccga ctggccgctc ctcaacgcgc
    26701 ttctgaatac ggcctcgggc gcaagctggg tgtcgattca caatggcggg ggcgtcggca
    26761 tcggctactc gctgcacgcc ggccacgtca cggtcgctga cgggaccgag tcatccgcgg
    26821 gctgcctgga acgcgtcatg acatgcgatc ccggcatcgg cgtcctgcgc cacgccgacg
    26881 caggttacga agacgccctt ctctttgcgg agcgggccgg cctcagattg cccatgaaag
    26941 agggacccaa acaggcctca tgagcgaaac gatcctcgtc cggggcgccc gtcagttgct
    27001 cacgctgcgg ggaccctcgg cgccccgccg cggcgccgct cttcgcgacc tcggcctgat
    27061 ccaggacggc tcgctcctta ttcgcgacgg cctcgtcgcc gccgccggcg ctacccgccg
    27121 gatcgaaaat ctggccgcgg cccgccacgc tacggagatc gatgccaccg gccgggtcgt
    27181 catgcccggc ttcgtagata gtcatacgca tctcatcttc ggcccgccgc ggctggacga
    27241 ttatgaaagg cgtctcgccg gccaggatta ccaccagatc gccgcagccg gcggaggcat
    27301 tctggtgagt gtgaaggcgg ttcggggcac atcgtcccgg gcccttctcc ggcagacccg
    27361 cctttcgctg catcggttga tccgccacgg cgccaccacc gtcgaggcga agtcgggcta
    27421 tggactcgac gagcctgccg agttgaagac tctgcgcgtc ctcgctgctc tggacggttc
    27481 cccgatcgat gtggtctcca cgtacctcgg cgcgcacgtc gttccgccgg aattcgcggg
    27541 ccgtccggag gagtacatcg gctgggtctg ttcgtacatg ctgcccctga tacggaggcg
    27601 ccggctcgcc cgctttgtgg acatctacat cgaacggggc gccttcgggc tggatgatgc
    27661 gcgccgctac ctcgagaccg cccggtcgct gggattcggt ctgaaagtcc atgccgagca
    27721 gttctcccat tcgggaggca ctcgcctcgc aatggaactg ggcgcggtca gtgtcgatca
    27781 tctggaacag gcgggcgccg aagacatcgc catggttgcc cagtcttcga cgatcgccac
    27841 cctccttccc ggctccgtct tccacctcgg cctcgatcgc tacccgcccg cccgagccct
    27901 catcgaccac ggggccgccg tcgccttggc taccgatttc aacccaggca caagccccac
    27961 gtgcagcatg ccgatggtcc tcgcgctcgc ctgcaatcag atgcgcatga cccccgccga
    28021 ggctgtctcc gcatccacca tcaacggcgc tcatgccgcc ggtgtcgcgg accgcgcggg
    28081 ttctctcgaa cccggcaaac aggccgatct gctcttgctc gacgtctccg actaccgcga
    28141 gatcccgtac catttcgggg tgaacccggt tgctgtgacg atgaagaaag gtcgtatcct
    28201 gtaccgccag ggggaggtca cgtgtcccga agacttatag agtgtgtccc gaacttctcc
    28261 gagggccgtg acgcctccgt cgtggatgcc atcacccgcg ctatccgctc cgtgcccagc
    28321 atcgtcctcc ttgatcggga gctcgacgcc gaccacaacc gttccgtgct caccttcgca
    28381 ggacctcctg acgccgtcgc cgaagctgcc ctgcgcggtg tcggggaagc tgtccgcctt
    28441 atcgatctca cccgccacac cggcgtccac ccccgaatcg gagccgccga cgtcgtacct
    28501 tttatcccca tcgagggcgt cgcgatcgcg gactgcgttc atctcgccga acacgtcggc
    28561 gaggagatct ggaagcgctt cggcgtgccc gtttaccttt acgaagcagc cgcccggcgg
    28621 ccggaccgcg tcaaccttga aaacatccgt cgcgggcagt tcgagggcct gcgcgaagag
    28681 attcaatcca acctggagcg ccggcccgac atcggcgaac ctcgcctcca tcccactgcc
    28741 ggagccaccg tcgtcggcgc gcgcaagttc ctcatcgctt acaacatcaa cctcaacacc
    28801 tccgacctcg acatcgcaaa gaaaatcgcg aagacgatcc gcttctcctc cggcggtttt
    28861 ccctacgtca aggcgatggg cgtgccgctc gcctcgcgca atctcgccca agtctcgatg
    28921 aaccttaccg acttcgagca gacccccgtg caccaagtct tcgaggccgt tcgcgccgaa
    28981 gccgcccgtt acggagtcga tatcgccgga agcgagatcg tcggtctcat cccccggaag
    29041 gccgtcgaaa tgacggctga gtttctcctc cgcgcggaga atctccgccc ggaaatcgtc
    29101 ctcgagaatc gcctcgccga agcgatggcc ggccggggcc tcgacgactt cctcgacgct
    29161 ctcgccgctc ccacgcccac gcccggtggc ggtagtgccg ccgcgtcctc gggtgcgatg
    29221 gctgccgccc tcggcgcgat ggtcgccggg atcgcgaagc ttccgggata cgactttacg
    29281 ccagatcgtc tcttcctcgc cgaagccgtc cgccgcgacg ccgccgcgta cgatgctgtc
    29341 gttgccgcct atcggcgtcc gaaagacgaa cgcgccccct tcgtcgaaga agcgttccac
    29401 caagccacgg cggtgcctct cgaggtagcc gagtgtgtgt cgcgggtcag gtcacgtttg
    29461 gaatccctcg ccctcgaagc ccctccgaag gtcgcctccg atgtcgaagt cgctctgctc
    29521 ctcgccgcgg ccgcgctttc cggcgctctg gccaacgtcc gcatcaactt gccttccata
    29581 aaggacgcaa ccttccgccg ttctgtggaa caacgcctcg cttcgataga atcgaagtag
    29641 tgctggtctc catcgcgccc aaactcgagc gtccggcgtg gctgcgcgcg cccgcgcccg
    29701 caggggacaa ctatcgcgag ctgaaatcgc tggtcgagaa cctgcgcctg cacaccgtct
    29761 gcgagagcgc cgcctgtccc aacgtcggcg agtgctggaa tcaccgcacc gccaccttca
    29821 tgatcctcgg caacgtctgc acgcgccgct gcggcttttg cgccgtcggg aaaggtgcgc
    29881 cgctccctgt cgactacgac gaaccccgcc gcgtcgccga agctgttgca acgctaggcc
    29941 tcaagtacgc cgtcgtgacc agcgtcaacc gagacgaccg caaggatggt ggcgccgaac
    30001 tgttcgccct caccattcgc gccatccgtg accgcgtacc cggttgcagg atcgaggtcc
    30061 tgatcccgga cttccagggc tcttacgccg ccatggacat cgtgcttgac gcggcgccgg
    30121 acgtaatgaa ccacaacacc gagaccgtcc cacgcctcta ccggcaggtg cggctcggcg
    30181 cgcgctacga gcgttcactc gacatcctgg cttattccaa gcgcgcccgc ccggagattc
    30241 cgacgaagtc aggcctgatg ctcggcttgg gcgaactgaa gggcgaggtc gtccctgtga
    30301 tgcgcgacct ccgtgcccat catgtggaca tcctcacgct cggacagtac ctgagcccct
    30361 cgccgaaaca cctcccggtc atccgcttca tcccgccgga tgaattcgcc gagtaccggt
    30421 tcctcggcga gcaactgggc ttccgtcacg tcgaggcggg accgcttgtc cgcagctcct
    30481 atcacgccgc cgaagccagc gcccggtcca ccgcctgaag cgtctcccca gcctcccgct
    30541 ctgtatgcac cgtggaggtg tagaaccgtc cgtccggcag cacgtaaacg ccttcggaaa
    30601 gtagcgcctt cgcgaaccgc gccagtttct cgcggtcgtc ctccagcgtg tcgcgatagt
    30661 cgcgcaattc ggccttctcc gtgaagtgca ccgcgaacgc cgccccgaat ccgcaaaccg
    30721 tcatcggcac gccccgcctc ttcgcgatgt cgcggatccc ctccatcact gctgcgccgg
    30781 tccggtttgc tcgtgccagc gccgcgccgc cgtcacggga aagctcctcg agcgtcgcgt
    30841 gggccgccgc cagcaccatc ggatttccat tgaagctgcc gccgaacgcg accccgccag
    30901 tgaacaacgt atcgagaata tcttgtctgc ccgccaccgc gctcagcgcc atcccgcccg
    30961 cgatcgcttt tccgaaggtc gcaaggtccg gctttacgcc gaagtggccc tgcgctccgc
    31021 cggctcccat gcgaaacccc gtgatcactt cgtcgaagat gagaaccgag ccattccgcc
    31081 ggcaaagctc cgcgatcgct tccaggtacc ccggtgccgg cggcaggcac ccgctgttgc
    31141 acagcactgg ttccatgatt acgccggcaa tctcgccgcc ccgccgctcg aacacccggg
    31201 ttacgctctc gacgctgttc cacggagcca ccacgacgtt ctcggcggcg ttcgtcacct
    31261 gcccccgcga ctcgaccacg acattgggtg ctgcgtaatc cccgacctga tccgcgcgcg
    31321 ggtggtagct caacagcaca gagtctagcc aaccgtggta gtggccctcg aatttcagaa
    31381 tcagattacg accggtgaag ccacgcgcga gacgcaacgc cagttgcacc gcctcgctgc
    31441 cgctcgacga gaacgccacc cgctccgcgc acggcgccaa ctgctgcacc ttctccgaga
    31501 ccaaatactc cagttcgtgc tgcgcgccga acgtgtggac gccgtccgcc tgtttgcgaa
    31561 gcgcctccac cagcgcgggg tgccggtagc cgagaatatt cggcccccac cccagcgtgt
    31621 agtcgatgta ctcgttgccg tccacgtccg tgagcctcgg cccgcatccg tccgcgaaat
    31681 acagcggcac cgggaacttc gcccggaatg ggctgctcac accccctgcg agcgactgcc
    31741 gcgcccgctc cagatactca cgcgattttc gccatctctg ttccgtcatt tcagttcgag
    31801 agtatcatgg ggacactatg gaaagccccg atcaagctac ccggcgcgga ttcctcggca
    31861 gtctcggcgc aacggccgtt cttgcccgcc agcgatctgc ggctccggta agggtcggag
    31921 tcgtcggcgg aggctttggg tcaagcttcc agtggcatct ccacccgaac gcgaaagtcg
    31981 ccgccgtctg cgacatacgc ccggaccgcc ttcagcggct ctccgaggtc taccgctgcg
    32041 gaactacact caagagcttc cgcgagttac tcaagcagcc cgagcttgac gccatcgccg
    32101 tattcacccc cgctcccctc cacgtctgga tggccaccga agcgatgaag gcgggtaagc
    32161 acgtcatcag cgctgtcccg gccggtattt ccatcgagga atgcgagcgg cttctcgaat
    32221 gcgtcaagag caccggcatg aagtacatga tggccgagac cagttactac cgtccagaaa
    32281 tcatgacctg cgtcgagtgg gctcgcgaag gccggtttgg aaccatcttt tactctgagg
    32341 cggaatacca ccacgaaggc ctgctcccct tgatgtacga cgatcgcggt ttccccacgt
    32401 ggcgctatgg cttccccccg atgctctacc cgacgcactc cgtcggcatg atcgtacccg
    32461 tcaccggcga acgcctcgcg gaagttcagg ccgttggctg gggcgacggc catgagatac
    32521 tccagaccaa tcagtacaag aacccgttct ggaacaccac cggattcttc aagacctccg
    32581 gcggccattc ttcccgcgtt tccgttttct ggcacgtcgc cgcaggggga acggagcgcg
    32641 ctcagtttta cggcagccgg ctttcctaca tcatggagcg ccccgaaggc tcgccgaaca
    32701 ccgtcattcg catcgcgcgc gacggcaaaa cggtgattga cgccaacggc taccccgagg
    32761 gcgacgtcgc gattgaggcc ttcaagcagc cggaccactt cgagaagctc cccgccccgc
    32821 ttcgtgtcaa aacgggccac ggcaactcgc acaccttcat cacgcacgag ttcgtctccg
    32881 cgatcctcga agaccgtcag ccggccgtca acgcctggga ggcgatcgcc tacaccgttc
    32941 ccggcatcgt cgcccaccaa tccgccctcc gcggcggcga gccgatgaag atcaaagact
    33001 acggcaaacc cccggcctga tcgcgcaggg agcggtcacc cacttctcac cagttcgtga
    33061 tcgatccgtc cggccggttg tagatcggaa tcgggcgcga gcgctccgtg acctccgcga
    33121 tcatcttcag ccgcttagga tcgaactcga ttcccagccc cggccgccgg ttcggccaca
    33181 gcttgccgtt ccggaagtcg aagttctgcg gcaggtgcgg cacttccctc ggccccgcgc
    33241 cgagcatctc catcagcacc ggacccgaga acaccccgca gcaatgcacc agcgaagccg
    33301 tggccagcgt cccggtgaag tgcggcacga gcccgacgta atgcgtctcg cacatcgccg
    33361 cgatcttcat gaactcggtg atcccgccac agttcggcac gctcactcgc gagtaatcga
    33421 tcaggtgctg ttccaccagc tcgttcagtt cccaccggtc accgaactgc tccccgaccg
    33481 caatcgggac cttcacctgc ggccgcagcg tcctgtagac gcccgggttc tccgaacgga
    33541 ccaggtcctc gacgaagtac ggctccaggt cttcaatcag cgtgcttagc cgcaccgcat
    33601 cggcgaagtc cagccgcgtg tggtaatcga tcgcccagtc gccgtccttg cccacgccct
    33661 cccggatctc acggcacatc tcgaccgtac gatggaccat ctcccgtgaa ttgaatggcc
    33721 ggccctcgct cggatcagcg acccccacgc gaaacgctcg gaaccccgac tcgacgcacg
    33781 cccgcgccgt ctcccgcagg ctgcccttct tcgggaaccc ggtcgaataa cattcgatgt
    33841 ggtccctcgc caaaccgccc agcagttcgt acaccggcgt gtccagggct ttgcccttga
    33901 tatcccacaa cgccaggtcg agcgcgccga gcgcgtgcag tttttcgcgt cccgccggat
    33961 agaagaaccc gcggtacatc atctgccaaa ggtggtcgat ccgccccggg tcctccccga
    34021 tgagcatcgc cgcgcactgc tcaatcgtgt ccttcgtgcc cccttcgccg atcccggtaa
    34081 tcccctgatc ggtctccacc gtcacgatgt tcgagctttg gttgtaaatc ggcctcgaat
    34141 tcggatcggc gtaatagcgg attcgcttga tcttcatcgg cccggctgcg gctcgtgcgg
    34201 cggaagttcg agccagccac gcccccgcgc ccatcgaaac ggaacgaaga aacgatcttc
    34261 tttccatcgc acctcctccg tacgagccgc cagcatatca ccgtacaatg ggaacgtggg
    34321 tttcttgcct gtcgacgagc agttcgccta tctgagaaaa ggatttgttg aggtcatccg
    34381 cgaggaggag ttgcgcgagc gcctcactgc gtcgttgagc accggccgtc ccctgaaggt
    34441 taaagtgggc ttcgatccca ccgccccgga cctccacctc ggccacaccg ttctcattcg
    34501 caagatgaag cacttcgagg acatgggcca caccgtcgtc tttgtcatcg gcgacggcac
    34561 ggcccgcatc ggcgacccca ccgggcgtaa catcacccgt ccgccaatga cccgcgaaga
    34621 gattctcgcc aacgccgaga cttacaagat ccaggtcttc aagatcctcg acccggcgaa
    34681 aaccgaggtt cggttcaaca gcgactggct cgatcagctc ggcttcgagt ccatgatccg
    34741 cctctgcgcc acgtacaccg tcgctcgttt gctcgagcgt gacgactttg caaagcgctt
    34801 caaagaaaac acgcccatct ccgttcacga gttactctac ccgctcgccc aggcctacga
    34861 ctccgttgcc ctccacgccg acgccgagct cggcggcacc gaccagaagt tcaacctcct
    34921 cgtcggccgc gacatccagc gcgacttcgg ccagccctcc cagatcgtcg gtaccacgcc
    34981 gctcctcgaa ggcacggacg gcgtcaacaa gatgtcgaaa tcgctcggca actatgtggg
    35041 catcaccgaa cctcccgagg tcatgttcgc caagctcatg agcatcagcg acgagctcat
    35101 gtaccgttac tacgagctgc tcaccgacgt cagcctcact gacatcgacc gtctgcgcga
    35161 tcgcattcgc accggcgatc tgcaccccat gcaggccaaa atcgacctgg cgagcctcat
    35221 cgtcaccgac ttccactccg ccggcgacgc cgcgcgcgcc gctgaggagt tcaaccgcgt
    35281 cgtccgccgc ggtgaagtgc ccgctgatct ccagacagtc cggatcgacg gagacgtccg
    35341 ccgcatcgac aaggtgctcg ccaaggcggg tctcgttgcg tccgtcagcg aagccacccg
    35401 caagctgaag gaaggcgcgg tcgagatcga cggtacgaaa gtggaaggat tgatccagct
    35461 tgcgtcgccc ggcgagtacg tcgttcgcgt cgggaagaac tggcgtcggg ttaccctctg
    35521 acagatgcct ccaatagaga agatcgtccc tctcgagacg tcgcacatcc cgttcgtcat
    35581 gcgcctcaac gaggccgccg gatggaacca gaccgaaaag gactgggcac gagtcatttc
    35641 cgtggaaccg gaggggtgct tcgcgctctt cgccgacggc gtcctcgcct ccaccgccac
    35701 cgcggtctgc ttcggccgcg cgctggcatg gatcggcatg gttctcaccg accccgcctt
    35761 tcgcgggcgc ggctatgccc gccggcttat ggaacacgcc ctcgaattcc tcgaccgccg
    35821 cggaatggag tggattaagc tcgacgccac cgacatgggc cgtccgctct acctcaggct
    35881 cggcttcgaa gatgaagcgc ccgtcgagcg ctgggtgctc gacaacgtgc ccgccatgcc
    35941 gcgggtcgat ctgcccccat acgagccgga ccctgctctc gatttcgaag ccttcggcgc
    36001 ctgccgttcc cgcatgctcg agctgctctc ggccgagggg gccgcgtcaa tcccgggcga
    36061 agggttcgcg atggcgcgcg ccggagcgaa ggcggattac ttcggcccgt gcgtgtgccg
    36121 gtcgccgcaa gcggcccgcc gtttcctcga gtggttcctc agccgcagcc ccggccgttc
    36181 cgccgcgtgg gacatcctgc ccaccaatgt ggaggccgtg cggctcgccc gtgagttcgg
    36241 tttcgagcgg cgcaggcagc tcgtccgcca agtgcgtccc gggcccgccg ccgggcagcc
    36301 gctcccgcac aacgacgcgt atgtcttcgc gatcgccgcc ttcgagtacg gatagatccc
    36361 atgccacgca agcgctacga gtggaaactg aaggaccggg cgattcagct cggcgaccgc
    36421 acactgatcg ccggcattct caacgttacg cccgactcgt tctccgacgg cggtaagtat
    36481 ctcgaccccg accgcgcctt cgcccgcgct ctcgaactcg aggaggaagg cgccgatatc
    36541 atcgacatcg gcgcggagtc cactcgtccc ggctccgcgc gcatctccga agccgacgag
    36601 ctccgccggc tcgtgcccgt tctgaagcgg ctcaagaaca agctgatggt gcctgtctcg
    36661 gtggatacct acaagtccgg agtcgccgcc aaggcgttgg aactgggcgc ggaaattgtc
    36721 aacgatccga ccggcttgac ctccgaccca cagatctccc gcgtggtcac cctaaacgac
    36781 gccggcctca tcctgaatca catgcgcgga acgcccgaaa cttgggcgaa gcttccgccg
    36841 cttcccgacg tcatggggac catcctccac gatctggacg cctcggtcca ccgcgcccgc
    36901 ggtgccggca tcgaccgttc ccgcatcgtc atcgaccccg gcctcggctt cggcaagcgc
    36961 aaggaccaga acttcgagat cctcgcccac ctctcgcaac tcgcccgcct cgacatcccc
    37021 gtcctgatcg ggccttcccg caaatcgttt ctcgcccaat cgaccgagca gctgaccgcc
    37081 ttcgccaccg cctcggccgt cgccgcatgc attctcggcg gcgcccacat cattcgggtc
    37141 cacgaggtgc gcgaaatgcg cgccgcggcc gacatcgctg acgccatcct gcagtcggtc
    37201 gccggcgagt gaccctacct caacgccgcc agcagttccc ctgtctgctt caacagattc
    37261 agccgcgccg tctcgagggc gtagttgctg tcgacgaacg cgatccactt ctcgtcctcg
    37321 gtgaaccgcg cctcctccac ctgccgcaac ggcgcgcgtc cctcttccat ctgcgccagc
    37381 aacacggaga cctgctcccg cgccacgtcg agatccagct ttgccacttc ccgtgccgtc
    37441 tccgcctgcc gcacttcacg gtacagccgc tgcgcgtcca gtgagatgcg gttccgagtc
    37501 ccctgtagtt cgatgcgcag ccgcgccgcg tcaccctccg cctgcgccgc ccgtgcgtcc
    37561 acccctggtc cggcgaacag cggaatctgg aacgacacgc cgagctgccc gttgtgccgc
    37621 tggaatttcc ggaaaaaatc ctcgtagttg ttgaacttcc ccagcagcgc atactgcgcc
    37681 accaggtcca gttgcggaag ccgtcctgcc cgctccgctt tgatctcgaa gcccttggcg
    37741 accagagccg actccagccg cctcacctct ttgctgttgt cgagcgccgc gcttaccgcc
    37801 gcctgttccg attcgggaag ctgcggggcg ggccgttcct ctaccgtcac tcgaatgcgg
    37861 tcgtccgcat tcagtccgag ggccgccgtc agtgcgcctt ctgcgtttgc ccgctctacg
    37921 tcgagcgcct ccgcccgctg ccgcgcctgc gccacgcgca ggtccgcgcg cttcgcctcg
    37981 atgggcagtt cgcgtccctc gctcacccgc gcccgcatca cgtcggcgac tcgtgtgagg
    38041 ctctccacct gccgccgcgc catctgcgcc actttcgccg cccgctccgc ctccaggtac
    38101 agtgtcgccg cgcgatgcgc cgcctcttcg cgtttggccg ctgtgtcgag cgtcgctccg
    38161 cgtgcgttct ctctcgcttg cgagatcagg tgcgactgtc gccggttgaa gacgtattgc
    38221 accgcctgcg cctggaagat cgatggagta gagcccgcca cactcagcgg gatgccgttg
    38281 ctgtacgcca ggccgctgcc cgccgcaact ttcggtatga acgggtcccg ggccagccgc
    38341 actccctgct cggccttgcc ttcggccagc cgcgccagcg ccaggtcggg gttctgcttc
    38401 aatgcgaggt ccaccgcttc gcggagagtc agcgttcgtt cctcaccggc ggcccccgcc
    38461 gccatcactg caaccgccaa caacatccgt atgtagttct gcatatttgt aatcctcgcc
    38521 atccgcccgg acgtgctact cgtaccgcag ggcttccgtc ggattcagcc gcgcggcgcg
    38581 gttggccggc agcagcccga acaccagacc gaccacgcag gacaccacga aggccaccac
    38641 gatcgacgcg attgagatcg gaatcttcac atccgagaag agccgcacgc tgaacggcaa
    38701 tgccagtcca accaggatcc cgagcagccc tccgcccagg ctgatcatta ccgcctcgat
    38761 aaggaactgt cgcaacacct cgcgccgcga cgccccgacc gccatccgca cgccgatctc
    38821 gcgcgtccgt tccgtcactg tcaccaacat gatgttcatg atgccgatcc cggagatcac
    38881 cagcgcgatc gccgaaatca ggatcagtac gagcgttagc accagcgcga tgctcttggc
    38941 cgcgtcgagg atcgccgtga ggttctccac caggtagcgc gcgcccggcc ggtgcctgct
    39001 ccgcagaatc tcgcccacgt tcgccgtcac cgcctccacg tcttcgggac ggcccacctg
    39061 cacatacatc gggtcgatcc gttccaccgg cgtaaaatac cggatcaccg tgatcggaat
    39121 caccaccgtc tcccgcgaca actccgacag cccgaaactc tccacccgtt ccttgaaggt
    39181 cccgatcacc gtgaactgga gcccgtgtac cttcaggacc tgcccgaccg ccgactgcgg
    39241 gctgccgtac aaccgccgcg ccagccgctc ggtcagcatc gccaccttct cgcgcaggga
    39301 aacgtcgctc cagtccagga accgtcccga cagcagcacc aggttgcgta ccgccgcata
    39361 gtgctcatcg gtgccgatga ccttcacgtc ctcttcgcgg ccctcgatca gcatccggtc
    39421 gtagttggtc atgacacccg tcgcggccac aatgcgcgag tcgagctgct gccgcaccgc
    39481 atcaacgtcg gagagcttga cgaagtccgc ctcgacctcc gccgccgact tccctccagt
    39541 ctcgtaatac gcgtaaacga tgttcgagcc gatgccgcgg atctgctcga ggatgtagtc
    39601 ccggctcgtc agcgaaatcg tgaccacgag aatcacggag gcgttgccga tgaccatccc
    39661 gagggaggtg aggaacgtgc gcagtttgtt ggcgcgtaaa gcgtggaggc tgaaccggag
    39721 cgcttcgccg aggtgcatgc ggagatcagc ccgtgcgggc ttccttgagc agccgttcgg
    39781 cctcacggcg cggcggatcg tccggcgtca gcgccagtgt caggtagcgt tcgagcaggc
    39841 tgcgcgccgt ccccagattg cgcttggaac gtacgtaagt gctcgcctgc gcgaacaaga
    39901 gcttcggggc gccgggcgcg atcttctccg cctgccggaa cgcggcgtcg ctctcttcgt
    39961 gccggccctg cttcgccagg aaccgtgcga ggtcgatcac ccggcccacc tgcccgggcg
    40021 ccagttcgat cgcccgctgg tattccgctt cggcggtctt gagatccttc cgcttttcgg
    40081 ccagcttggc gaagaagaag tgatactcgg ccgggttgag atccttcgag cgttcagcta
    40141 gctccgcggc cttttccgcg ccgccgccca gaaagccggg cgcctcaagg tagtattcaa
    40201 acaggtcgcc cactgcctcc aggttgcgcg ggtccagcgc gacggccttc tcgaatgcct
    40261 gccgcgcttt ctttgcctgc ccgggcgcgg tgaggaatga cgatgtctcg gcccgccgtc
    40321 cgtgggcttt gcccagccaa ttggcgtaca cggagttctt cggttcggcg gcaagggcct
    40381 tctcaaacgc ttcggtggcg cccttgaaat cgtccagcat gtaccggcag cggccggcca
    40441 gttcccacct cgcgccgtct gcttgcggaa gggactcgat gaccttcagc gcctccgcgt
    40501 accgggtctt ctgatagagc gagcgcgcct gctccagcgc cgccgtctcg gcgagcgcag
    40561 agcccgccac caacgccagt actgtcagca acttgcgcat ccccacccct ccattctacc
    40621 cacgaaaaac ggtggctggc tctactgtcc caagcctgtt ggaaaaccag tggctggctc
    40681 cactgtccca agcgtcgtct gcgtgtggac agtggagcct gtcactggtt ttccccttcc
    40741 cctaacttct cgacgtatcg gtagccaccg cccgtttcat cctggtcttg agtctcgggc
    40801 aggcgttact ccacaaccgc ctgcgatgcc agcacctgga acgtgccgtc aagattcagc
    40861 ccgcccgtgg gatgcagttg ggccatgaac accccgacca attgctcctt gggatcgatg
    40921 aagaacaccg tgtagtaaaa gcctccccaa ccgtacagcc ccggtgatcc cagctcatcc
    40981 atcggaacct tgccgcccga aaccccaaaa cccagaccaa aactcaggtc cggagccact
    41041 ttcccaagct ggtcgtgcgt catcagttcc accgttttgc ggctcagcag ccgctttcca
    41101 tcgagcacgc cgccgttcag catcatctgg cagaaccggc catagtcggc agcggtcgag
    41161 ttcagcccgg cgcccccgct gaacagagtc ttcggtccgc ggtacgggta gtcggccgag
    41221 tattcgaacg gcccctccac gatcggcttg gccggaaacc ggctgattgc tttgccctcg
    41281 tcccgcatgt acaccgaggc caagcgggag actttctccg aaggaaggta gaagtgggtg
    41341 tccttcatcc ccagcggttc gaaaatccgc gttcgaaaga actcgtccaa cgacttgccc
    41401 gaaaccacct ccaccagccg tccgagcaca tcaatcgaca ggctgtattc gtacttgtca
    41461 ccggggtgga agagcagcgg ttgcgctgcc aggcgcttga cactctcagc aatcgttcca
    41521 tcgtactgaa gcaggccgtg ggcgacgccg gcgtcgcggt atcgggggcc gagatcgggg
    41581 ttccagtggt agaccagccc ggacgtatgg ttgagcagat gcttgatggt gatttcccgt
    41641 gccgccggaa tcgtgtacgg ctcccccgcg gccggtttga caagcacttt cgggttcttg
    41701 aactcgggga ggtatttcga caccgggtcg ttcagcagga agcggccctc ctcgtagagc
    41761 atcatcaccg ccaggctagt aatcggtttc gtcatggagc agatccggaa gatgttgtcc
    41821 gtctgcatcg gcttgcccga ctcgacgtcc gccattccct gcgccttgag ccacgccacg
    41881 cggcccttcc tggccaccag cgtcaccgcg cccgcgatcc tcttcgcctc gatctctcgc
    41941 tggacggccg cgccgattct ctccaaccgc accgccgaca ggcccacctc ctccggcttg
    42001 gctgtcggca gaccctgccc tgccgccggc agacacgcaa tcgccgcagc caatgcgaac
    42061 agcacgcgca gaatccgaga tgaaaagcga acgttcacaa tattcctcct gacccgaaac
    42121 cagagagtgt acacccggag accaatcagc cgcgcaggag gcgtcacctg acacgccagt
    42181 tgttcggggc gacggcccgg tgaactgaga tggacgttcc ggtctagtac tgcatagtat
    42241 tcatcgccca tttacttctg ctgtctggtg ccctagaatc tgaaaattgc gagctttgct
    42301 cgcccgaacc gagatgtgga ggggtgccta actgtgggaa aacgtagact tatccgtcgt
    42361 tttgttgcgc tggccgctgt gtccctcctg ctaccgttgt gttccgcaca actgcggcag
    42421 gtgcccgagc cggccgtcgg agccgccgca gcttcgcaag acgactacat cgttaccttc
    42481 cagcctggtc tcagccaggc ggagcgcgcg gctgcggtgg tgagagccgg tgccaacgcg
    42541 cgattcaact tcagcatcat taacgcggta gcggtgcggg ttccaaacgc caacgtgctg
    42601 gcggcgattc agcgggacat gcgcgtggta agcatcgtcc cagatcggcc ggtccatgca
    42661 tttgggaagc cgaccaagcc gggtggcggc tccagcgggc aggtggtacc ggaaggtgtg
    42721 aagcgggtaa atacacccgt cttgggttcg tccgacggtt ccggtattgc cgtcgcggtg
    42781 ctggatacgg gcatcgacct gaaccatgcc gatctggcga tcggatcaag ccgatttgat
    42841 gcctttgggg gttcctgcca ggatgggaac ggccacggca cccatgtcac aggaatcatt
    42901 gcggctctcg acaactcgat cgatgtcgtg ggtgtggctc cgaatgcgac gccttattgt
    42961 gtgaaggtgc tggacgacgg cgggtctggg agcgactccg ccgtgatcgc tggactggat
    43021 tgggtctaca atcggaccga cctgcagatt cgcgtggtga acatgagttt ggggcgggac
    43081 aaagtggacg gggacatggc gctcgacagc cccatgcgcg cagcgattcg gcagctatac
    43141 gccaaaggaa tcatagttgt cgctgccgct ggaaacgaag cggccaaaga ggtgattcag
    43201 aaagtccctg ccggctatcc ggaggtgctc gcagtcgcca gcaccacggc caaggacggg
    43261 agcaacgcgt gccgtctcct tccaaatcca atccggaagg atacggcatc ctttttcacc
    43321 accgacggaa gcctcgattc cacatcggac ggctcgatcg gcgttaccat ctcggcccct
    43381 ggagaggacc aggagaatgt aagccgggga tgcttgatca gttccgtggg aattctctcg
    43441 ctgaaagcgg gcggcgggac cattcggtat tccggcacga gcatggcggc gccacacgtt
    43501 accggcattg tcgctcgcgt tctccagaaa gaattggccg ttgggcctga gggtgtgcgc
    43561 ggctattttc aagcgaacgc ttcggccaaa cagacggcgc cactggactc gcccaccagc
    43621 acttatacgt tcgatgggga gcgagaaggg gtggctgtag caccttagtt ctttgatctt
    43681 accggtttgc attgagaagg gcggtccacc agtgtacacc cggtcacgag ctgaactctt
    43741 ttttgttggc cctgttctca gtcacttaca gccgccgccc ctcgggaacc atggacacgg
    43801 ccccttacac tgaaatcgag gaggcggttg aggaaaccgc ctcgacgtat cggtggccac
    43861 catacgtttt catcgaagtg gactctgacc gcggcgctcg tgagggcttg cggtggtggc
    43921 tttcatgccg ccatcgacgt gccagggtgg gatcggtccc cttgtgaagc cgacacttcc
    43981 ttgcccggcg ggccggggta ggaccccggt ccgccgatga cgaaacgatc tttactcctt
    44041 ccgactccct tttccccgtc tcttgagcct acgtgtgccc atttcccaca ctgtgagtgg
    44101 cccggccttc acgctgtgcg ccgaacgtct ctgacaaacg gcatcaccat acgcgtgcac
    44161 ggccacccca cgtcggcggc ggattcgaag gcagttactt ccgaatcgtg aagcgttaga
    44221 ctggtgaaat atgaacccaa accctgcatt tgaccgaatc acggtggagc ccggcaaatg
    44281 cggtggaaag ccgtgcatcc gcgggatgcg cattacggtg cgcagggtcc tcgagatctt
    44341 ggccacctac gccgaccggc aagagatctt ccgcgagtat ccgttcctcg aagaggaaga
    44401 cttgcagcag gcgctccgct atgcggcggc ctcggtagat gaggaattgg tcgacttgaa
    44461 ccgcgtcgca tgaaactggt cttgctcgat cagggcctcg caccgagggc agccacacta
    44521 cttcgatcag agggctggga ggcgcttcat gtgagtgagg tgggcctgca ccgggcggaa
    44581 gatctcgaaa tcctggaatt cgcgcggcaa cgcgggatgg tgtgtgtcac tctagatcac
    44641 gattttcaca cgcatctggc gctctccctt tccgggagcc cctcggtgat tttcgtacgc
    44701 gtggcgggac ttgatgctta ccagcaggtt gtgctgatta aagccgtttg ggaagtttgc
    44761 ggggaagcca tcgccgaagg ggcggcagta tcaactgacg gaaccactgt gcgactgcac
    44821 aagctgcctc tgaaataacc tacggcgcgg ttggtgggtc cccggtagca ggttcggttt
    44881 tcaagtccgg ccgccggacc attgcaagtc tcttctgtcg tacccttaca agtcaattct
    44941 gctgcaagtg gcacggcctt cccgggtggc ccacttctgc ggcagagccg gagctccatt
    45001 ccgatccacg accgaaggga gtggtcaggc ctgtcgactg acagcgtcac cgtctacgtg
    45061 cacagccagc cggcgacgat cgggacggcc ttcatcgagg aggcggcgga gcaggtcgga
    45121 gacgtcctgc cggaacttcc tccggctggc gcatcggcga gatagatggc ggcgtgagcg
    45181 gctcaggcgg cagcatcggc ggatcaggga gcaggaccgg accgttcagt cccgaaaaga
    45241 caagcccggc gaatagcgcg agtatcacgg ccaagcactg caagggtttc atcggagttc
    45301 cgtgcccgat cgatctccgt tccgatcata tctccgcagt tcaccttccg gaccacaccc
    45361 cctctccccg tgaaacaatg aatggccatg aggtcaaagg tcctgattgt cctgctcatc
    45421 gccatcgtca ctgcggctgt ggccgctgcc gcgctggcgc cgtcatcatt cagtactgag
    45481 gcggtcaatt cctacgcgga gcttcggcgg ctcttcccga atccgcccgc gagctatagc
    45541 accgcgccgt tcttcgtctg gaacggcgag atgaccgagt cggagatcga caagcatctc
    45601 gcggattacc acgcccaggg catacgggcc ttcttcatcc acccgcgccc cggcatgatc
    45661 acgccgtacc tctcggaccg ctggttcgcc ctctgccgct acaccgtcga caaggccaaa
    45721 cagctcggca tgcaggcttg gctctacgac gaaaactcct accccagcgg cttcgccgga
    45781 ggtcacgtgc ccgccgaaat gcccgagtcc tggaacgaag gccagggcct cgtgctccat
    45841 aaactcaccc agttccccgc ggaccccggc aaatacaaaa tcattctcaa gaaggcggcc
    45901 ggctccttcg aagacgtcac cgcgtcggcg tcccccgaac agggccgcca gggtgactac
    45961 ctcgccttcg atctctcttt ctacgaaaag agcccctggt acggcggatt cacctacgtc
    46021 gatctcatcc gccccggcgt caccgaaaag ttcatcgagg tgaccatgcg cggctacgag
    46081 cgcactctcg gccccgacct cggcaaaacc gtccccggcg tcttcaccga cgagcccaac
    46141 atcgtcccgc ccgccgacga gaattgcatc cgctggacgc ccgacctctt cgatcaattc
    46201 aaaaagcgtt ggggttacga cctccgtcct cacctgcctt ccctcttcga gcagaccggc
    46261 gagtggcagc gcgtccgcca taattactac gccttgctcc tcgacctttt catcggccgc
    46321 tgggcaaagc catggaaccg ttatgccgat tcgaaaggca tcgcctggac cggccactac
    46381 tgggagcacg agtggcccaa ccctcgccag ggtccggaca acatggccat gtacgcctgg
    46441 tctcacgtgc ccggcatcga catgctcttc aaccagttcg ccgaaaacgt cgccgcccag
    46501 ttcggcaaca tccgctccgt caaggaactc gcgagcgtcg cgaaccaggt cggcgcgcgc
    46561 cgcgcgctca gcgaaaccta cggcggcgcc ggctgggaac tccgcttcga ggacatgaag
    46621 cgccttggcg actgggagta cgcgctcggc gtcaacttga tgaaccagca cctctccttc
    46681 cagaccatcg tgggcgcccg caagtacgac tatccccagt ccttcaccta tcacgagccc
    46741 tggtggagcc actaccgcgt gctcgcggac tacttcgcgc gcctctcgct cgccttgagc
    46801 gccggcgagc aggtgaaccg cgttctcgtg atcgagccga cgtcatcggc atggatgtac
    46861 gcttccgcgg gacaggccga cccgcgcctg atgcagctcg gcgagacctt ccaggctttc
    46921 gtgacgcgct tcgaaaagct ccagggcgag tacgacctcg gttgcgagaa catcatccgt
    46981 gaccgcggca aagccgacgg cgcgcgcttc gtcgtgggcc gccgcgcata cgatctggtc
    47041 gtgctcccgc ctggcacgga gagcctcgac gctgccactg tgaagctcct cggcgaattc
    47101 cttcgaggcg gcggcaaagt gttgtctttc gtcgagccgc ccgcgcgggt ggacggcgcc
    47161 gaagacgcgc gcgtgacaca gctcgcgtcg caattcgatt cgagctggac gcgcgccgat
    47221 tctctcgacg atccgcaggc gcgcaagatg ctactccccg ccggcatcac ggacgtcgcc
    47281 ggtcaactcc ttcaccagcg ccgcgttctc gacggcggtg agctgctctt cttcgccaac
    47341 cacagcctcg aacaaccggc gcgggcgacg atcaagatcg ccggaaaatc cctgatgcgg
    47401 ctcaacccct tcgacggctc gaccactgcc caccccgcac ggcgcgaagg caactcgctc
    47461 gtcttctccg tcgaactgcc gccggccggc agcctgctgg tcgctgcgct aaaagaaggt
    47521 cccgcgccgc cggctccatc cccggcgcca gtcgagacgc ccgtcgcggc ctcaggatcg
    47581 ctggcagtaa ggcgcctcgc cccgaacgtg ctccagatcg actactgcga cgtgaaactc
    47641 ggcggcactg tcaccgagga cctcttcacg tacaacgcta cggacaaggt cttcaagcac
    47701 tacgggcttg aagcgaaccc ctggaacacc gccgtccaat acaaaacctc catcctcgac
    47761 atggaccggt ttccggctga ctcgggcttc gacgccgctt tccatttcga tgtcgccgcg
    47821 ggcgtcgatg cttccgcgtt tcgcgccgtc gtcgagcggc cgcatctatg gaaggtcacc
    47881 gtgaacggca agccagttac cgcgcgccct gacgaatggt ggcttgaccg cgcgtttgga
    47941 gtctacgaca tcggtgcgca cgttaccgcc ggccgcaaca ccatcctgct cgccgcgcgg
    48001 cccatgtcca tccatcacga aatcgagccg gtgtacgtgc tcggcgagtt cgcgctcgac
    48061 gcgcagccgc gcggttggaa gatcgttccg ccccagagcg ccgctcccgg cgcgtggaag
    48121 gaacaggggc tgccattcta ttcgaacgcc gtctcctaca cccgtatcta cacgcttaag
    48181 aaaggcgcct tcaaggtgag gctcggcaag tggcacggga ccgtcgccga agtcaaagtc
    48241 aacggcaagc ccgccggcat catcggctgg cagccttacg agcttgacgt cagccgcttc
    48301 gtccgcggtg gcgacaacac ggtcgaagtc atcgtcatcg gctcactcaa gaatctgctc
    48361 ggcccgcacc acggcaagat cagccgcggt ttcgtttcgc cgtggctctt ccgcaacgcc
    48421 cccgagcaca tgccgcccgg ttcgggctac gacctcgact cctacggcct cttcggcgac
    48481 atcgagttga tcgaatcccg gccccggtag gccgcgatgc ccgggtacca cgagtctgac
    48541 atcaaccggc tgctcaagac gttccgccac caggaggcgg accgcgtgcc gcacctcgag
    48601 ttctgggtca ccagcaagcc cgtctatgag tacgtgctcg gctacccgct tcaatacgac
    48661 gtcgtcgacg cgcgcgtcgg cggccagtcg atcacaccgg aagatcatgt tctcttcgcc
    48721 cggaaattgg gcatggacgc ggtcgcgtgc aatttctcct ggcgcccgaa caatgtcttc
    48781 cagaaggccg ccgatggcac tgagcactat accggcggca cggtcagaac ccgagacgat
    48841 tttagccgcc tcgaaccgcc gcccgcgctc
//
LOCUS       DATIFQ010000361         2636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1459717, whole genome shotgun sequence.
ACCESSION   DATIFQ010000361 DATIFQ010000000
VERSION     DATIFQ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459717"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VLE22_17590"
     CDS             complement(<1..233)
                     /locus_tag="VLE22_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16272.1"
                     /translation="MKSPRVVLGLLLLMLLATRLCHLGILWAEEDLPLAAAVQMVHGK
                     SLYRDFWFDKPPLTAAINLLWGAHTGWILRIAGA"
     gene            complement(230..1114)
                     /locus_tag="VLE22_17595"
     CDS             complement(230..1114)
                     /locus_tag="VLE22_17595"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HSB16273.1"
                     /translation="MIKSAIVPVAGFGTRLLPATKSQPKEMLAVARKPIVQYVVEELV
                     ANCVEEILFVTGRNKGSIENHFDHDPELMRILNVGNKQELLEELKFEELQAHFFYTRQ
                     RFQKGLGDAILCGENFAGEEPFVVALGDSILGLHANSSAVGRMAELFESKHASCVIAV
                     EEVPADEVRHYGVVKPADGNGEEFRVADVIEKPKPENAPSNLAIAGRYVFSPLIFDMI
                     RRVKPDHRNEVQLTDAIRLMCEEGKRVLALKLPPGEKRYDIGNFSSYFETFVEFALAD
                     PIYGPNLRKALERMLEKK"
     gene            1141..>2636
                     /locus_tag="VLE22_17600"
     CDS             1141..>2636
                     /locus_tag="VLE22_17600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HSB16274.1"
                     /translation="MRDVQHTLYTENLPMPLRTKTHTARQFFSRHGLLSRWHPNYEFR
                     QGQLEMAEAVEAALNDRRHLIVEAGTGTGKTLAYLVPAILSGKRIVVSTGTKNLQEQL
                     FFKDVPFLQQHFDRPLRVCYMKGRNNYACRQKIYDAEKEPILAGLEEVADFQIIRDWE
                     KTTETGDRAEIRRLPEVSTAWAKIDARRDLCTGQPCPQFERCFLTRMHQRAVESDIII
                     VNHHLFFADLAVKEEEFGGIIPEYAAVIFDEAHEIEDVAGQYFGISVSSHQIQDLRRD
                     TASLARRKQFGSDELDRVLETLEQRAERFFSLFGNGEGRRGFTAQQAFLEEHEDDYGD
                     LLRCLELVGAHLESIQQAPEEVIPLYRRAREIADNLKFWMGGADRSFVYWVERRGRGC
                     FLQATPIDVSPILSERLFGKIDVAVLTSATLAVAGSFDYTEGRLGMRDARTLIVAGQF
                     DYQNQALLYVPQHLPDPRSLAFSGMAADEIKRLLALSHGRAFVLFTSY"
BASE COUNT          484 a          852 c          833 g          467 t
ORIGIN      
        1 gcgccggcaa tgcggagaat ccatccggtg tgcgcccccc acagcagatt gatggcggcg
       61 gtgagcggcg gcttgtcgaa ccagaagtcc ctgtagaggg atttgccgtg aaccatttgg
      121 accgcggcag cgagcggcag atcctcctcg gcccacagaa ttccgaggtg acagagccgc
      181 gtggccagga gcatgagtag cagcagcccg aggaccacgc ggggggattt cacttcttct
      241 ccagcatccg ctccagcgct ttgcgcagat tcggaccgta gatcgggtcc gccagcgcga
      301 actcgacgaa cgtctcgaag tagctcgaga agttgccgat gtcgtaacgt ttctcgcccg
      361 gcgggagctt cagcgcgagc actcgcttac cctcctcgca catcagccgg atggcgtcgg
      421 tgagctggac ctcgttgcgg tggtccggct tgacccggcg gatcatgtcg aaaatcagcg
      481 gcgaaaagac gtagcggccg gcgatggcca ggttgctggg cgcattttcc ggcttcggct
      541 tctcgataac gtccgctacg cggaactcct cgccgttgcc gtcggccggt ttcacgacgc
      601 cgtagtggcg gacctcgtcg gcggggactt cctcgaccgc gatgacgcaa ctggcgtgct
      661 tggactcgaa taactcggcc atacgcccga ccgcgctcga gttcgcgtgc agtccgagga
      721 tcgaatcacc gagcgcgacc acgaacggtt cctcgccggc gaagttctcg ccgcagagga
      781 ttgcgtcgcc gagtcctttc tggaagcgct ggcgggtata gaagaagtgg gcctggagct
      841 cctcgaattt gagctcttcg agcagctcct gcttgttgcc gacattgagg atgcgcatca
      901 gctccggatc gtgatcgaag tggttctcga tggagccttt gttgcggccg gtgacgaata
      961 ggatttcctc gacgcagttg gcgacaagtt cctcgacgac gtactggacg atcggcttcc
     1021 gcgcgacggc gagcatctcc ttgggctggg acttggtggc cggcagcagc cgcgtgccga
     1081 accccgccac gggaacgatg gcgctcttga tcaccttctg aatataacgg aaccggcacg
     1141 atgagggacg tccagcacac cctctatact gaaaatctgc cgatgccgct gcgcacgaag
     1201 acgcataccg cccgccagtt tttctcgcgc catggactcc tgtcgcgatg gcacccgaac
     1261 tatgagttca ggcagggtca gctcgaaatg gccgaggcgg tcgaagcggc gctcaacgac
     1321 cggcgtcatc tgatcgtcga ggccggaacg ggaaccggaa agacgctggc ctacctcgtg
     1381 ccggccatcc tctcgggcaa gcgcatcgtc gtctccaccg gcaccaagaa tctccaggaa
     1441 cagcttttct tcaaggacgt cccgtttctc cagcagcact tcgaccggcc cctccgcgtc
     1501 tgctacatga agggccgcaa caactacgcg tgccggcaga agatctacga cgccgagaaa
     1561 gagccaatcc tggccggcct cgaagaggtc gccgacttcc agatcatccg cgactgggag
     1621 aagaccaccg agaccggcga ccgggccgag atccgccgcc tgccggaagt gagcacggcc
     1681 tgggcaaaga tcgacgcccg ccgcgatctg tgcacgggcc agccgtgccc gcagttcgag
     1741 cgctgcttcc tcacccggat gcaccagcgc gcggtggaaa gcgacatcat catcgtcaac
     1801 caccacctgt tcttcgctga cctcgcggtc aaagaagaag agttcggggg catcatcccc
     1861 gagtacgccg ccgtgatctt cgacgaggcg cacgaaatcg aggacgtcgc cgggcagtac
     1921 tttggcattt cggtcagcag ccaccagatc caggacctgc gccgcgacac ggcgtcgctg
     1981 gcgcggcgaa aacagttcgg ttccgatgaa ctggaccggg tcctcgagac cctcgaacag
     2041 cgcgccgagc gcttcttcag cctgttcggc aacggcgaag gccgccgcgg cttcaccgcc
     2101 cagcaggcgt ttctggagga gcacgaggac gactacggcg atctgctccg ctgtctggag
     2161 ctcgtgggcg cgcacctcga atcgatccag caggcgcccg aagaggtgat cccgctctac
     2221 cgccgcgccc gcgagatcgc cgataacctc aagttctgga tgggaggcgc ggaccgctcc
     2281 ttcgtctatt gggtggagcg ccgcggccgc ggctgcttcc tgcaagcgac gccgatcgat
     2341 gtttccccga tcctgtccga gcggctgttt gggaagatcg atgtggccgt gctgacctcg
     2401 gccacgctgg cggtggcggg cagcttcgac tacaccgagg gccggctcgg catgcgggac
     2461 gcccggacgc tcatagtggc cgggcagttc gattaccaga accaggcgct gttgtacgtg
     2521 ccgcagcacc tgccggaccc gcgaagcctg gcgttctccg ggatggccgc cgacgagatc
     2581 aagcggctgc tcgcgctcag ccacggccgg gcctttgtac ttttcacgag ttatca
//
LOCUS       DATIFQ010000362         2595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_312296, whole genome shotgun sequence.
ACCESSION   DATIFQ010000362 DATIFQ010000000
VERSION     DATIFQ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312296"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1207)
                     /locus_tag="VLE22_17605"
     CDS             complement(95..1207)
                     /locus_tag="VLE22_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16275.1"
                     /translation="MTVKRRSILLGALGAPALAQTPATPAVTSELDRRNDAYAVELEQ
                     YFRRVLVEEYPARAAKLWNRSYESIPAFEMSVEPNRRRYREIFGPPPLKPSGAAVRKP
                     SKMVRDIKAEWLSIPLGPLKAEGLLAVPAGASGRVPLVIAQHGIDSFPERVFGVGDDR
                     NLYHDYGHALLREGYAVLAPINLRTIARRNRIERLARLGGTSMPGIELVRMQALLDEV
                     LKDERIDPRRIGLWGISLGGMATMFWMPLERRIKCGIVTAWYNHRRNKMVIPDKRYSC
                     FLETDEEHAFFHGWLTEFTDSDAACLICPRPLQIQHGKKDGIAWWPQVIEEFEAAKEH
                     YRKLGIQDRIEMDLHDGGHEIRLAPGLAFLNKWLKA"
     gene            1239..2159
                     /locus_tag="VLE22_17610"
     CDS             1239..2159
                     /locus_tag="VLE22_17610"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HSB16276.1"
                     /translation="MPRALFVGDINVDVMMGGMESLPVVDREVVCKTYDVVMGASTVI
                     CACTYASLGGDSSMAGLAGRDDYGEFMIRGLGELGVKTGLVKRTDRVRTGVTVNLIYQ
                     STRTQVTYQGTLAEFDGSELADSILDGFNHVHFGGIYLLHKLRPEITRLLKAAKARGV
                     TSSLDPQWDPSEKWEGMDEWLPLLTYLFVNEDEALSLARAASPKEACKALTARTRMPL
                     VKVGPAGSLVCVDGDVRAIPSYKVDVVDTTGAGDSFDAAFLFATIEKKMEPVAACAFA
                     NAVAARSCMFVGGVNARTSYEEAVRFGEER"
     gene            complement(2241..>2595)
                     /locus_tag="VLE22_17615"
     CDS             complement(2241..>2595)
                     /locus_tag="VLE22_17615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSB16277.1"
                     /translation="MKFSNETMKAESIFFYMMATGLALAPIALWMTDFSKPVNWGFKG
                     PYLSALIQVLNAIGALCLVYAVRYGKAIIVVPMTALAPVLTVIISLALYRVIPHPVIL
                     GGMVFATIAIYLMAE"
BASE COUNT          485 a          711 c          929 g          470 t
ORIGIN      
        1 agtgaggggg agaggcgaga aaacctcggt acagacacca atttcgcaac tgccgcgaaa
       61 ttagtgtcag tcccgggttt tccctgtttc cagttcaggc tttgagccac ttgttgagga
      121 aggcgaggcc gggggcgaga cggatttcgt ggccgccgtc gtggaggtcc atttcgatgc
      181 ggtcctggat accgagtttc cggtagtgct ccttggcggc ttcgaactcc tcgatgactt
      241 gcggccacca ggcgatgccg tccttcttgc cgtgctggat ctggagcggc cgggggcaga
      301 tgaggcaagc ggcgtcgctg tcagtgaact ccgtgagcca gccgtggaag aaggcatgct
      361 cctcgtccgt ttcgaggaaa cagctatagc gcttgtccgg gatgaccatc ttgttgcggc
      421 ggtggttgta ccaggcggtg acgatgccgc acttgatgcg ccgttcgagc ggcatccaga
      481 acatcgtggc catgccgccg aggctgatgc cccacaggcc gatgcggcgc gggtcgatgc
      541 gctcgtcctt gaggacttcg tcgagcagcg cctgcatgcg cacgagctcg atgccgggca
      601 tcgaggtacc gccgagacgc gcaaggcgct cgatgcgatt gcgccgcgcg atggtgcgga
      661 ggttgatggg ggcgagcacc gcatagccct cgcgcaggag ggcgtggccg taatcgtggt
      721 agaggttgcg gtcatccccg acgccgaaca ctctctccgg aaagctgtcg atgccgtgct
      781 gggcgatgac gagaggaacg cggcccgatg ccccggcggg gacggccaag aggccttcgg
      841 ccttgagagg cccgagggga atggagagcc actcggcctt gatgtcgcga accattttgc
      901 tcggcttacg gacggcggcg cccgacggct tgagcggggg cggcccgaag atctcgcggt
      961 agcggcggcg attcggctcg acgctcatct cgaaggcggg aatgctttcg taggaacggt
     1021 tccacagctt cgccgcccgg gcgggatatt cctccacaag aacgcggcgg aagtactgct
     1081 ccagttcgac ggcgtaggcg tcgttccggc ggtctagctc gctcgtaaca gcaggggtag
     1141 cgggcgtctg cgccagcgcg ggagcgccca atgcgccaag aagaatcgat ctacgtttca
     1201 cggtcatcag ttgcctccgg atgctaccat ctgattctat gcccagggcg ttatttgtcg
     1261 gcgatatcaa cgtggacgtg atgatgggcg gcatggaatc gctgccggtg gtggacaggg
     1321 aagtagtgtg caagacctat gacgtggtga tgggcgcgtc gacggtgatc tgcgcgtgca
     1381 cgtacgcttc actcggcgga gactcgtcga tggcggggct ggcgggacgg gacgactacg
     1441 gggaattcat gatccggggg ctcggagaac tcggcgtgaa gaccggcctg gtgaagcgga
     1501 cggaccgcgt gcggacgggc gtgacggtga acctgatcta tcagagcacg cgaacgcagg
     1561 tcacctacca ggggacgctg gcggaattcg acgggagcga actggcggac tcgattctcg
     1621 atggcttcaa ccatgttcat ttcggcggca tctacctgct gcacaagctg cggccggaga
     1681 tcacgcggct gctgaaggcc gccaaagcgc ggggcgttac atcgtcactc gacccacagt
     1741 gggacccgag cgaaaagtgg gaaggcatgg acgagtggct gccgctgctg acgtacctgt
     1801 tcgtcaacga ggacgaggcg ctctctctgg cgcgcgccgc gagcccgaag gaggcgtgca
     1861 aagcgctcac cgcaagaacg cggatgccgt tggtgaaggt gggaccggca gggtctttgg
     1921 tgtgcgtgga cggcgatgtg agggcgattc cgtcgtataa ggttgacgtg gtcgatacta
     1981 cgggggccgg cgattcgttt gacgccgcgt ttctgttcgc gacgatcgag aagaagatgg
     2041 aaccggtggc ggcatgcgcg ttcgccaatg cggtggcggc gcggagctgc atgtttgtcg
     2101 gcggcgtcaa cgcgcggacg agctatgagg aggcggtgcg gtttggggag gagcggtaga
     2161 gaggcagaga ccaggcggga caggcagctc tgcccgctcg cgaacaacgc tcagggcaga
     2221 gtgggcctgt cccgaggcta ctattctgcc atcaggtaga tggcgatggt ggcgaagacc
     2281 atgccgccga gaatgaccgg gtgggggatg acccggtaga gcgccagtga aatgatcacc
     2341 gtcagcacgg gcgcgagcgc cgtcatcggc acgacgatga tggctttgcc gtaacgcacg
     2401 gcgtagacga gacacaaggc gccgattgcg ttcaacacct gaatcagggc cgagaggtac
     2461 ggtcctttga acccccagtt gaccggcttt gagaaatccg tcatccacag cgcgatcgga
     2521 gcaagagcca ggccggtcgc catcatgtag aagaagatac tctcggcctt catggtctcg
     2581 ttggagaact tcatc
//
LOCUS       DATIFQ010000363        13889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_312571, whole genome shotgun sequence.
ACCESSION   DATIFQ010000363 DATIFQ010000000
VERSION     DATIFQ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13889
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312571"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VLE22_17620"
     CDS             <1..525
                     /locus_tag="VLE22_17620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB16278.1"
                     /translation="RTGAQIDFRIGKLPLNGSLPPGAHQAVFRVAQEALANIGRHARA
                     QNVSVTLDNVNAGLALIIRDDGQGFDSSDSPRGMGIANMRARAEEFGGTFSLNTLPGG
                     GTSVTFSIPCAVEASWDYKQRALMWGVLLLIGVVATAWSRSTLLASWQILPAVAFFRE
                     TIAWLRTRKQSEAM"
     gene            522..1163
                     /locus_tag="VLE22_17625"
     CDS             522..1163
                     /locus_tag="VLE22_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB16279.1"
                     /translation="MNDAISIVVVDDHRVVARSLKAYLESFPDMKVVGMAASGEELLE
                     HLEEWKPQIVLQDLLIPGGMDGIETTRRICERAPSVRVIALTASMDEARMMGVLRAGA
                     VGYVRKDADPEILLAAVRAVARGKTYIDPSIRRQILQAADAQDDLTPRETEVLRQLVL
                     GRSNKEIADVLSITEETVKTHVGNVLAKLQATNRSQAIVHALKRGLVSLDELE"
     gene            complement(1172..1954)
                     /locus_tag="VLE22_17630"
     CDS             complement(1172..1954)
                     /locus_tag="VLE22_17630"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB16280.1"
                     /translation="MGSLYGQDLAYIQAAAFGRLAKGAAPEIIRLLKSATIQIRKVVD
                     VGCGAGPLTSALVEVGFEVTGIDSSAELLAIACAAVPRAHFVNASIYDEEIPACEAIV
                     ALGESLTYHAEGVDADSLVQSFFQRASDILPPGGMLIFDVIELGEPSLAGRFWSSGEE
                     WAVLVDTWEDQDFRTLVRNIETFRRVDHLYRRGYEIHRVRLFDTGILCSQLAACGFVT
                     ETAQFYGTQRLGPRRRAFFSTRIGQDLSVKTGTTAPFGRGAG"
     gene            2067..3314
                     /locus_tag="VLE22_17635"
     CDS             2067..3314
                     /locus_tag="VLE22_17635"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSB16281.1"
                     /translation="MTGVTFTTKGALRKSALRQANERLALDAIRQNPAISRSDLARIT
                     GLSPSSVSFIVNRLLRAHLLREEKVGNHSQVGRRPTALYLVREAMLAIGVEISQNESR
                     IALADVHGEILRHKSIPWHANHDILGQRIHAALRSLVEETASQKVLGIGVALPGTIDR
                     NTGRVIAAENLNWLNVDLDALIHGRLTLPFYYENSAKLSALAESWFTEPGQRALRDFV
                     FITPTNGLGTGIITNGHLLQGSSAIGGEFGHVMLYPDGRKCSCGNQGCWEQYGCDAAL
                     LRVYAENGGTQDDGAELSPAEVVRRARGGEEAARNTLRQVARDLGLGFVNLVMVFNPE
                     ALVVGGYIADAWEFIEDIVWQALRSRAPHYALAGLRVFPSNHATDSSLLGAVALVYSH
                     FFTCFEHDAEGAHSTPVLMHATV"
     gene            3564..6851
                     /locus_tag="VLE22_17640"
     CDS             3564..6851
                     /locus_tag="VLE22_17640"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB16282.1"
                     /translation="MNGFTTRPLVVLLSVLFLTLPVGLFGQATQGSILGSVTDPTGAG
                     VPGANVTVKNEGTNFVRTITTDQSGDYRVSGIEPGIYRVTVTATGFKGFEQTRIDLAS
                     SQAKRIDVKLEVGDVATTVTVEGGVTQIETETASLSNLKTARDFGQLPLSVFGRGWAN
                     ITNVTAGIQSTSGFEVNGARDTANNFTADGISVNDMISSRNTANGFSGDIETFQEIKI
                     LTANTSAEYPQVAQFAAVSKQGTNEFHGTLYWGIFNSNFSARAWSDRQPPSFTNHNMF
                     AVNGGGPVVIPGLYDGRNKTFFFASYSGARYRVGNRSYTIVPTAAMRGGDFSALAGKI
                     NLVDPLNNGLPFTDNRIPSNRISSVSQKVQDLLYPDPNQPGQGAYGLDNNFYADPGYS
                     YDSNVYSFRVDQKISDKNILYTRVGLTIDNKDFQRGPLKNGWGAGSYDGNIPGRSVVV
                     SDTHTFSPTFVNEVKLGFNRTYSESTDVNFGVDVLSQLGIEGIENPGNDPAIGSMPQF
                     SFGGAVPVALSTSRNQGYTAQNTYQIIDNVSWFGGRHNVKFGTDIRRLQVNNQNKPVS
                     LRGAYSFDDRLTGLAYANFLLGWPSGATRAIARPNAYPRSTYWGFYWQDDFKLHQRVT
                     LNFGVRYEYQTPWVEKFDRMFTFDPKSGSMVTAGSSIPTDLVPAVAATLPIITATAAG
                     LPERSLMHNDSNNWSPRIGLAIRPFGDTTTVVRAGFGVYTQFWPGLLGLNQTGGPWQS
                     TESFILENPNTPAIQFPTPFKTTSAFSGIQSISGVGARFPNENTYQWNLSLGRQIWGI
                     ALDIGYVGTRSLNVPFNEDLNLLYPSTTPFSAARRPYPRFNAANLVQTGGSSTYHGFT
                     IQADRRMAGGLWFNANYTYAKGLTDVDLRSYSTGAQQNQYARYLERADDGNLRRQQLR
                     FSYLYDLPFGRGQRLLNGIPGPLNFFLGGWQLAGITTMVTGARLSPAFSGTDPAYTNQ
                     FGGRPDRLGDGNFDADSMRDSIQSGKPIFDKAAFVRPETGRGYYGNSARYILTGPGQV
                     VWNAGVHKNWKFAERMQMQFRWEAFNAFNRANFSNPTTNIQSGSFGLVTVAGSGRSML
                     FGLRLDY"
     gene            complement(6954..8906)
                     /locus_tag="VLE22_17645"
     CDS             complement(6954..8906)
                     /locus_tag="VLE22_17645"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB16283.1"
                     /translation="MNCRCLPVAGLLLLAGCQPSNKEAAAPALRRLNLVLITIDTLRA
                     DRVGCYGYAKIETPNLDSVAGRGVMFENAVAQAPLTSPSHASMMTGLLPTVHKVRDTG
                     GFVLRSSDTTLAKILQQQGWDTAAFVGSSVLKKRFGFDQGFAVYDDEMGENRERRAGE
                     VVDRAIGWLGSRSEKPFFLWVHVYDPHLPYDPPAGFREKYKDRPYDGEIAYTDQELGR
                     LFAAIGKNTLLAVLSDHGESFSEHGEYAHGIFLYDTTLRIPFLMAGPGVPAGLRVKQQ
                     ARTVDLLPTVLELMGGKAPQAVQGSSLVPAFSGKEVPTEYSYAETLFPKLNMGWTELR
                     AIRTNRWKFVRAPRPELYDLAQDPAEISNVIATHAAEARDLEAKLDAAAGSAREKVEA
                     AASDRRTMEQLRSLGYLGGSSQREYELTGKGIDPKDRIGVLKLLHFAVYEESGLPLSQ
                     RVTMMRQAIAEDPSNPALYSSLGDLYRDGGDRNHEIKLYQDAVKRGVRSAGLYSRLGS
                     LYLRQGRKSEAIPLLEQAARWNPSDYDSLEHLAVAYRETGRIEDAERTCNSILKSGEE
                     YAPAYNELGMASFQKGDVAKARDYFEKAAQLDPIYQMNLGRLYKLAGDKERARTAFEA
                     FLAARGSSEEYRRLLPQVKQELAQVR"
     gene            complement(8986..12669)
                     /locus_tag="VLE22_17650"
     CDS             complement(8986..12669)
                     /locus_tag="VLE22_17650"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB16284.1"
                     /translation="MNTLHRSFVLVLLVCVLTSMAWSQSSTGSVRGTVQDQTDAIIPG
                     ATVILTNTATNGESRTTSNAVGLYVFPAVRPGPYKITAEFAGMKKFEGTMTVQVQKGE
                     TVNIVLYPETTQTVVQVEDVTPMLTADSQTLGHVLERTRIEQLPINGRQVMSLLNTVP
                     GLTFDEGGDLRTLGERTGTHDISLDGAALTEPVYAGGTVRRQPSLESIQEFKVDMNAV
                     SAKYPRQTNIILTTKGGGNTLHGSLFETNRDNYYGWARRREDGNTPGKFIRNEYGGTV
                     GGPVWIPKVYNGKDRTFFFFAFEQFKQRSGAIGRYKVPTEAMRNGDFSGLVDSSGTLS
                     RLYDPFQTDPVTHLRPQFAYQGKANAIDPALASPLWKYFAEQLPMPNQAGVNPLVANN
                     YFGPRPDKYNQWTWTARFDQRLGQNDQVYARLSNSYSKRFRAAANGVPTLDGLGNSRT
                     DYFPDKSLASNWTHSFSPTFFNEFMFSMSRTTGGNYTGENGYYADMLGLPNPGHQVGF
                     PVISTMGLSSGNYFQPVNGTQQWFNHFILDDNATKILDNHEIQFGVHFRYDQLTYLPQ
                     QQRSAGNVSWQAIGTALFDPNFPNRSRAVLNTGHRLGSAFLGLANYEYRVNKGKYYMG
                     QNEDAAYIQDNWKVTPRLTLMGGLRWQFSPYPKDKYDIFSAFDLNNLAIVLGNELDTF
                     YKLGVTSPAYIAYLESNGAKFESAEQGGFPKRLVNNNWFDIGPHVGFAYRAFEGSKAF
                     VLRGGFATSYFPIPIWGWNDRMRNNAPFTGFYANPWMTQASASPDGLRNYGLVSVPKY
                     IAGQNTTNAITLNDLNPSSIIAGEDAYNAAWFNPDQPSAKVHDWNLTMEKEIMKDTVV
                     RVAYVGSRLSNQDSYDDLNQMKPDYVWYTTTGQPFPETSPELRPYSNTPYGEIQEYRR
                     DGWGNSNGATFEIERRFSKGYGFQVFYQMVNYSKAAAHGWYGDSAVDPVTSYLPGVVP
                     ADHKERMRLLLYARDTTVPKHEVRWNWIAELPFGQGKPVLRNAPKVLDYIVGGWQISG
                     MGRLRSHYFSLPTDIYPTGSKIEYYGHKYPIQDCRSGECIPGFLMWNGYIPAHQINQP
                     DGIMGVPSNYTPAGQPLWPYPANYNSLNEDIDPNYGYYGSNDVFVKLNDGTLQPVDRT
                     SGDLHPWRNQPIASTRSWSTDMSIFKNFPLGERVGLKFQADFFNVFNTPGNEWNPGND
                     GIVLTNYSMQDARQLQLTMRLSW"
     gene            13214..>13889
                     /locus_tag="VLE22_17655"
     CDS             13214..>13889
                     /locus_tag="VLE22_17655"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSB16285.1"
                     /translation="MRRAEAITRAFPGDITTLAEWQRRRPEIKKQYLYALGLDPMPAK
                     TPLKAQVTGGFERDGYRVENIVFQSMPGLYVTGNLYLPAGKQGRLPTVLYLCGHSPGP
                     WGNKVRYQHHGIWFARHGYAAFLIDSIEFGEVPGLHHGTHDLEMWDWLSLGYTPAGPE
                     VWNAIRALDYLETRPEVDPKRVSVTGISGGGAITWFAAAADERFQVAAPVCSTWTVEH
                     HTALDAV"
BASE COUNT         2605 a         4380 c         4145 g         2759 t
ORIGIN      
        1 cgcaccggcg cgcaaatcga tttccggatc ggcaagcttc ctctcaacgg gagcttgccc
       61 cccggcgctc accaggcggt cttccgcgtc gcgcaggaag cgctggcaaa tatcggccga
      121 cacgcacgcg ctcaaaacgt atccgtcacg ctggacaacg tgaatgccgg attggcgctg
      181 attattcgcg atgacggcca agggttcgac tccagcgatt ccccccgcgg aatgggcatc
      241 gccaacatgc gtgcccgggc agaggaattc ggcggaacct tctcactgaa caccctgccc
      301 ggcggcggaa catctgtgac attctcgatt ccctgcgcgg tggaggcgtc ttgggactat
      361 aagcagcgtg cgctgatgtg gggcgtgttg ctgctcattg gcgtcgttgc cacggcctgg
      421 agcaggtcaa ccctcctcgc ctcgtggcag atactccccg ccgtcgcctt cttccgcgaa
      481 acaatcgcgt ggctgcggac gcggaaacag agcgaggcga tgtgaacgac gccatctcca
      541 tcgttgtcgt ggatgaccac cgggtcgtgg cgcgtagcct gaaagcctac ctggaatcgt
      601 ttccggacat gaaagtcgtc ggcatggctg cctccggcga ggaattgctc gaacacctcg
      661 aggaatggaa gccgcagatc gtcctgcagg acctgctgat tcccggcgga atggacggga
      721 tcgagaccac acgacgaatt tgcgaacgcg cgccgtcagt gcgtgtgatt gccctcaccg
      781 cgtctatgga cgaggcgcgg atgatgggcg tgctgcgagc gggcgcggtc ggatatgtgc
      841 gcaaggacgc agatcccgaa atcctcctgg ccgccgtgcg tgccgtcgcc cgagggaaga
      901 cctacatcga cccgtccatc cgccgccaga ttctccaggc cgcggatgcg caggacgatc
      961 tcacacctcg tgaaaccgag gtgctccggc agttggtgct cggccggagc aacaaagaaa
     1021 tcgccgacgt cctctcgatc acggaagaaa ccgtgaagac ccacgtcggt aacgtcctcg
     1081 ccaaactgca agctacaaat cgatctcaag cgatcgtcca cgccctgaag cgcggcctcg
     1141 tgtcactcga cgagttggag tgacatcgaa ctcaaccagc gccgcgacca aagggagcgg
     1201 tggttccggt ttttactgac aggtcctggc cgatgcgagt agagaagaaa gcccgtcggc
     1261 gtggaccaag gcgctgcgtg ccgtaaaatt gcgccgtctc cgtcacgaac ccgcaggcgg
     1321 cgagttgact gcagagtata ccggtgtcga aaaggcgaac tctgtggatc tcgtatccgc
     1381 gcctgtagag gtgatcaacc cgccggaaag tttcgatgtt cctgacgagc gtccggaaat
     1441 cctgatcttc ccaagtgtcc accaatacag cccattcctc gccggaactc caaaaccggc
     1501 cggccagtga cggctcgcca agctcgatta catcgaagat gagcatcccg ccgggaggca
     1561 ggatgtcgga cgcacgttga aagaagctct ggaccaggct atcggcgtct acgccttcgg
     1621 cgtggtacgt caaagactcc cccaaggcga caatggcctc gcatgcgggg atctcttcat
     1681 catagataga cgcgtttaca aagtgcgctc tcgggacggc tgcgcacgca atcgccagaa
     1741 gttcagcgga actgtctatc cctgtcactt caaagcccac ctcaacgagg gccgaagtca
     1801 acggtcccgc accgcagccc acatctacga ctttgcggat ctgaatcgtg gcagatttga
     1861 gcaatcgtat gatctcgggc gcggcgccct tcgcaagccg gccgaacgcc gccgcctgaa
     1921 tatatgcgag atcctgtccg taaagcgacc ccatgaattc aatgacagtc tatcgcgctc
     1981 aacagcccgc cccgtgtcga gtgttcaaag ccctccttga caacttattt ttttgactga
     2041 aataatggcg gacttgggac tacgaaatga ctggtgtcac cttcacaacc aagggagctc
     2101 tcagaaaatc cgcacttcgc caagcgaacg aacggctcgc gctcgacgcg atccgtcaga
     2161 atccagccat ctcccgctcg gatctcgcaa gaatcaccgg gctctcgccg agttcggtga
     2221 gtttcattgt caaccgcctc ctgcgggccc accttctccg tgaagagaaa gtgggaaatc
     2281 actcgcaggt gggacggcgg cctaccgcgc tctatctcgt gcgcgaggcc atgctcgcca
     2341 tcggcgtcga aatctcccaa aacgaatcgc gcatcgccct cgccgacgtt cacggtgaga
     2401 tcctgcgcca caagagtatc ccctggcacg cgaaccacga cattctcggt cagcgaatcc
     2461 acgcagccct tcgttccctc gtcgaggaaa ccgcgtcgca aaaagtgctc ggcatcggcg
     2521 tcgccctccc cggaacaatc gaccgcaaca ccggccgcgt catcgcggcc gagaatctga
     2581 actggctgaa cgtggatctg gatgcgttga tccatggccg cctcacgctg cccttctact
     2641 acgagaacag tgccaagctt tccgctcttg ccgaaagctg gttcaccgag cccggtcaac
     2701 gggcactccg cgacttcgtc ttcatcacgc cgaccaacgg ccttggcacc ggcatcatca
     2761 ccaacgggca ccttcttcag ggttcatcgg cgatcggcgg cgagttcggc cacgtcatgc
     2821 tctatcccga cggccgaaag tgctcgtgcg gaaaccaggg ttgctgggag cagtatggct
     2881 gcgacgcagc gttgcttcgt gtatatgccg agaacggcgg cacgcaggat gacggcgccg
     2941 agttgagtcc ggccgaagta gtgcgccgcg cgcgcggcgg tgaggaggcg gcgcggaaca
     3001 cgctccggca ggtggcgcgt gacctcgggc tcgggttcgt caaccttgta atggtgttta
     3061 acccggaggc gctcgtcgta ggtggctaca tcgccgacgc ctgggagttc atcgaagaca
     3121 ttgtctggca ggcgttgcgg agccgtgccc cgcattatgc gcttgccggg ctgcgcgtgt
     3181 tcccttcgaa ccacgctacc gattcctcgc tgctcggtgc cgtcgccctc gtctattctc
     3241 atttcttcac gtgctttgag cacgacgccg aaggcgctca tagcactccg gtcctgatgc
     3301 atgccaccgt ttgacggaat gtaggccgtc gacgtagtgt agatctccag atcggctgac
     3361 caggtttctc aacaaggtcc gagaattcgt tgtgtcttag tgtctccgtg gtgaatcctg
     3421 cattagggga ctccttgtcg cgcacctggc tttccttgac aatcgagtga atactatata
     3481 cagtattgga aataacaagc gaattgcgcc accgctgggg gttgtcgatg gcgcgaatac
     3541 caacaacaag aggaggctct cacatgaatg gattcaccac acgtccgctg gttgttttgc
     3601 ttagcgtcct gttcctgacg cttccggttg gtctgttcgg tcaagcgacg cagggcagta
     3661 tcctcggttc ggtcaccgac ccgactggcg cgggtgtacc cggtgccaac gtaacggtga
     3721 agaatgaagg cactaacttc gttcgaacca tcactaccga ccaaagcggc gactaccgcg
     3781 tgtccggtat cgagcctggc atctaccgag tcaccgtcac cgccactggt ttcaaaggct
     3841 tcgagcagac gcgcatcgac ttggccagta gtcaggccaa acgaatcgac gtcaagctgg
     3901 aggtaggcga cgtcgctact accgtcaccg tggagggcgg cgtcacccaa attgagaccg
     3961 aaaccgcgtc cctctccaac ctcaaaaccg cgcgcgactt cggccagctt ccgctgagcg
     4021 tgttcggccg cggctgggcc aacatcacca acgtgaccgc cggcatccag agcacgagcg
     4081 gctttgaggt gaacggcgcc cgcgacacgg ccaacaattt caccgccgat ggcatctccg
     4141 tgaatgacat gattagcagc cgcaacaccg ccaacggctt ctctggcgat atcgaaacgt
     4201 tccaggagat caagattctc accgcgaaca cctcggccga ataccctcag gtggcgcaat
     4261 tcgccgcggt gagcaagcag ggcaccaatg aatttcacgg cacgctgtac tggggaatct
     4321 tcaacagtaa tttctctgcc cgcgcctggt ccgaccgtca gccgccgtcg tttacaaacc
     4381 acaacatgtt cgccgtcaac ggcggtggtc ctgtcgttat tccgggcctc tacgacgggc
     4441 gtaacaagac gttcttcttt gcctcataca gcggcgcccg ttaccgggtc ggcaaccgct
     4501 cgtacacgat cgtgccgacg gccgcgatgc ggggcggcga cttttccgcc ctcgccggta
     4561 agatcaatct cgtcgatccc ctcaacaatg gcctgccgtt caccgataac cgtattccct
     4621 ccaaccgcat cagtagcgtt tcgcagaagg ttcaggacct gctttatccg gacccaaatc
     4681 agccggggca gggggcttac ggcctcgaca acaacttcta tgccgatcca ggctacagct
     4741 acgactccaa cgtctactcg tttcgagtgg accagaaaat cagcgacaag aacatccttt
     4801 acacgcgggt cgggctcacc atcgataaca aggacttcca gcgtggcccg ttgaagaacg
     4861 gctggggagc cggcagttac gacggcaaca ttccgggccg cagcgtggtc gtctccgata
     4921 cgcacacgtt cagcccgacc tttgtgaacg aagtcaagct cggattcaac cggacgtaca
     4981 gtgagtctac cgacgtcaac ttcggtgtcg acgttctctc ccagctcggc atcgagggta
     5041 tcgagaatcc cggaaacgat cccgccatcg gcagcatgcc gcagttctcc tttggagggg
     5101 ccgttccggt cgccctcagc acgagccgga accaggggta cacggcccag aacacgtacc
     5161 agatcatcga taacgtgtca tggttcgggg gacggcacaa cgtcaagttc ggaaccgaca
     5221 tccgcaggct gcaggtgaat aaccaaaaca aaccagtaag ccttcggggc gcctactcct
     5281 tcgacgatcg cttgaccggt ctggcctatg cgaacttcct tctcggctgg ccttcggggg
     5341 ccacgcgcgc gatcgcccgt cccaacgcct acccgcggag cacctactgg ggcttctact
     5401 ggcaggacga tttcaagctg catcagcgcg tcaccctgaa cttcggcgtc cgctatgagt
     5461 accaaacgcc gtgggtggag aagttcgatc ggatgttcac cttcgacccg aaatccggga
     5521 gcatggtaac cgccggttct tcgatcccca cggatctggt gcccgccgtc gccgcgacgc
     5581 tgcctatcat caccgccact gcggccggct tgccggagcg cagcctgatg cacaacgact
     5641 ccaacaactg gagcccccga atcggtctcg cgatccgccc gtttggcgac accactacag
     5701 tcgttcgtgc cggcttcggc gtctacacgc agttctggcc ggggttgctc gggctcaacc
     5761 agaccggggg cccgtggcag tccaccgagt cgttcatcct cgaaaacccc aacacgccgg
     5821 cgattcagtt tccgacgccg tttaaaacaa cgtcggcctt ctccggcatc cagagcatca
     5881 gtggcgtcgg cgcacgcttc ccgaacgaaa acacatacca gtggaacctc tcgctcggcc
     5941 gccagatctg gggtatcgcg ctggatattg gttacgtcgg cactcgttcc ctcaacgtgc
     6001 cgttcaacga ggacctgaat ctgttgtacc cgagcacgac tccgttcagc gcggcgcggc
     6061 gcccctatcc gcggttcaac gccgccaacc tcgttcagac cggtggctca tctacctacc
     6121 acgggttcac gatccaggcg gaccgccgca tggcgggggg tctttggttc aacgccaact
     6181 acacgtatgc gaagggtctg accgacgtcg acctgcgcag ctacagcacc ggtgcgcagc
     6241 agaaccaata cgccaggtat ctggagcggg ccgacgacgg aaacctccgc cgccagcagc
     6301 ttcgcttcag ctatctctac gacctgccgt tcggccgcgg ccagcgcctt ctcaacggca
     6361 tccccgggcc gctgaacttc ttcctcggcg gatggcagtt agccggcatc acgacaatgg
     6421 tgaccggtgc gcgtctgagc cctgccttct cgggcaccga ccccgcctac accaatcagt
     6481 tcggcgggcg cccggacagg ctcggcgacg gaaacttcga cgccgattcc atgcgagaca
     6541 gcatccagag cgggaagccg atcttcgaca aggcggcctt cgtccgtccg gagaccggac
     6601 gtggttacta tggcaactcc gcccgctaca tcctcacagg gccgggacag gttgtatgga
     6661 acgccggcgt tcacaagaat tggaagttcg ccgaacgcat gcaaatgcag ttccgatggg
     6721 aagcctttaa cgcgttcaac cgcgccaact tctccaaccc gaccaccaac attcaaagcg
     6781 gttccttcgg attggtcacg gtcgccggct ccggccggtc gatgctcttt ggcctccgcc
     6841 tcgactactg atgcaccttc aacgcggggg cctgtggtcc ggcccccgcc ttcccgccgt
     6901 tttcaccaaa cgcatcacca cacacagtca tagcaacatc tttcctctgt gccctatcga
     6961 acttgcgcca gttcttgctt cacctgcggc agaagccggc ggtattcctc gctcgatccc
     7021 ctggcggcaa gaaaggcttc aaaggccgtg cgggcgcgct ccttgtcccc tgcgagcttg
     7081 tacagccggc cgagattcat ctggtagatc ggatcgagct gggccgcttt ctcgaagtaa
     7141 tcgcgcgcct tggctacgtc ccctttctga aacgacgcca ttccaagctc gttgtacgcc
     7201 ggggcgtatt cttcacccga cttcaggatc gagttgcagg tccgctcggc gtcttcgatc
     7261 cgtcccgtct cgcggtaggc gactgccagg tgctccaggc tgtcgtaatc cgacgggttc
     7321 caccgcgccg cctgctccaa cagcggaatc gcctcgctct tgcggccctg gcgaagatag
     7381 agcgagccga ggcgggagta cagccccgcg cttcggacac cacgcttcac cgcgtcctgg
     7441 tacagcttga tctcgtggtt gcggtctccg ccgtcccgat acagatcgcc caggctcgag
     7501 tagagggccg ggttcgatgg atcttccgcg atggcctgcc gcatcatcgt gacccgctgc
     7561 gataatggca gccccgactc ctcatacacg gcgaaatgca ggagtttgag aacgcctatg
     7621 cggtccttcg gatcgatgcc tttgccggtc agttcatatt cgcgctgcga ggaccctccg
     7681 agatacccga gggacctcaa ctgctccatc gtgcgccggt ccgacgccgc ggcctcgact
     7741 ttctcccgcg cgcttccggc ggcggcatcg agtttcgctt cgaggtcccg ggcttccgct
     7801 gcgtgagtcg caatcacatt cgagatttca gccgggtcct gcgccaggtc gtaaagctcg
     7861 ggtcttggcg cgcggacgaa cttccagcga ttcgtgcgga tggcccgcag ctccgtccaa
     7921 cccatgttga gcttggggaa cagcgtctcg gcgtaggaat actccgtcgg gacctctttg
     7981 ccgctgaacg ccggaacgag actgcttccc tgcacggcct gcggcgcctt gccgcccatc
     8041 agctcgagca cggtcggcag caggtctacg gttctcgcct gctgcttcac gcggagacct
     8101 gccggcacgc ccggccccgc catcaggaac ggaatgcgca gagtggtgtc gtagagaaag
     8161 atgccgtgcg cgtactcgcc gtgttcggag aagctctcgc cgtgatcgga caacaccgcg
     8221 agcagcgtgt tcttgccgat ggccgcgaag aggcgcccga gttcctggtc cgtgtaggcg
     8281 atctccccgt cgtacggccg gtccttgtat ttttcccgga acccggcggg cgggtcgtac
     8341 gggaggtgcg ggtcgtacac gtgaacccag aggaagaacg gcttttccga tcgcgagccg
     8401 agccacccga tcgcgcggtc cactacctca ccggcgcggc gctcgcggtt ttcgcccatc
     8461 tcgtcgtcgt agaccgcgaa gccctggtcg aatccgaagc gcttcttcag caccgaagag
     8521 ccgacgaacg cggccgtgtc ccagccctgc tgctggagta tcttcgccag ggtcgtatcc
     8581 gacgaccgca ggacgaatcc gcccgtgtcc cggaccttgt gcacagtggg gagcaggccg
     8641 gtcatcatgc tcgcgtgcga gggagaggta agcggcgcct gagcgacggc gttttcgaac
     8701 atcacgcctc gccccgcgac gctgtcgaga ttcggagttt cgatcttcgc gtagccgtaa
     8761 catcccacgc ggtcggctcg cagcgtgtcg atcgtgatca ggaccagatt gagccggcgc
     8821 agcgccgggg cggcagcttc tttattagac ggctggcagc cggcgaggag aagcaggccg
     8881 gcgacgggga gacagcggca attcattgac gaagactgtt attttaccgc ggcgaacaga
     8941 aaggctgtgg gggcgaggca tgctcgcccc caccattcag aggaattacc aggaaaggcg
     9001 catcgtgagc tgcaactggc gcgcgtcctg catgctgtag ttggtcagga cgatcccgtc
     9061 gttgccgggg ttccactcgt tgcctggcgt attgaacaca ttgaagaaat cggcctggaa
     9121 cttcagaccg acccgctcgc cgagcgggaa gttcttgaag atggacatat cggtgctcca
     9181 gctccgggtg gacgcgatgg gctggttcct ccagggatgg aggtcgcccg aggtcctgtc
     9241 caccggctgc aaggtgccgt cgttcaactt cacgaacacg tcgttggagc cgtaatagcc
     9301 gtagttcgga tcgatgtctt cgttcaagct gttgtagttc gccggatacg gccagagagg
     9361 ctgaccggcc ggcgtgtagt tggacggcac gcccatgatg ccgtcgggct ggttgatctg
     9421 gtgggcgggg atgtagccgt tccacatcag gaagccggga atgcactcgc cgctccggca
     9481 gtcctgaatc ggatacttgt gcccgtagta ctcgattttg ctgccggttg ggtagatatc
     9541 ggtcggtagg ctgaagtagt ggctgcgcaa ccggcccatg ccgctgatct gccagccgcc
     9601 cactatgtag tcgaggacct tgggcgcgtt gcgcaggacc ggcttgccct gtccgaacgg
     9661 aagctcggcg atccagttcc agcggacctc gtgcttcgga actgtggtgt cacgcgcgta
     9721 aaggaggagc cgcatacgct ccttatggtc ggcggggacg acgcccggca agtacgaagt
     9781 caccgggtca actgcggaat cgccgtacca gccgtgtgcc gcggctttgc tgtagttcac
     9841 catctggtag aacacctgga agccgtagcc tttcgaaaac cggcgctcga tctcgaacgt
     9901 cgcgccgttg gagttgcccc agccgtccct gcggtactcc tgaatttcgc cgtagggcgt
     9961 gttgctgtag gggcggagtt ccggcgaggt ctcggggaag ggctgcccgg tggtggtgta
    10021 ccagacgtag tcgggcttca tctggtttag gtcgtcgtag ctgtcttgat tgctcaggcg
    10081 cgatccgacg tacgcgacgc gcacgacggt gtccttcatg atttccttct ccatcgtcag
    10141 gttccagtcg tggaccttgg cgctgggctg gtcgggattg aaccaggcgg cgttgtaagc
    10201 gtcctcgccg gcgatgatgc tgctcgggtt aaggtcgttc agcgtgatcg cgttcgtggt
    10261 gttctggccg gcgatgtact tcggaacgga gaccagtccg taattgcgga ggccgtctgg
    10321 agaagcgctg gcctgcgtca tccaggggtt tgcgtaaaaa ccggtgaatg gcgcgttgtt
    10381 gcgcatgcgg tcgttccagc cccaaatcgg aatcgggaag tagctcgtgg cgaagccgcc
    10441 gcgcaggacg aacgctttac tcccctcgaa cgcgcggtag gcgaagccga catgcggccc
    10501 gatgtcgaac cagttgttgt tgaccaggcg tttggggaag cctccctgct cggccgactc
    10561 gaacttggcg ccgttggatt cgagatatgc gatgtacgcc ggcgaagtga cgccgagctt
    10621 gtagaacgta tcaagctcgt tgcccaggac gatcgccagg ttattcaggt cgaacgcgct
    10681 gaaaatgtcg tatttgtctt tcgggtaagg gctgaactgc cagcgcaggc cgcccataag
    10741 ggtcagccgc ggggtaacct tccagttgtc ctggatgtag gcggcatctt cattctgccc
    10801 catgtagtac ttgcccttgt tcacccggta ttcgtagttg gccagtccga ggaacgcgct
    10861 gccgagccgg tgtccggtgt tgaggacggc ccggctgcga ttggggaaat tgggatcgaa
    10921 gagcgccgtg ccgatggctt gccacgaaac gttgccggcc gagcgctgct gctgcggcag
    10981 gtaggtcaac tggtcgtagc ggaagtgaac gccaaactgg atctcgtgat tgtcaaggat
    11041 tttcgtggcg ttgtcgtcca gaatgaaatg gttgaaccac tgttgcgtgc cgttcaccgg
    11101 ctgaaagtag ttgccgctgc tcaagcccat cgtggagatg actgggaacc cgacctggtg
    11161 gcccgggttg ggcagtccca gcatgtccgc gtaatagccg ttctcgccgg tgtagttccc
    11221 gccggtagtg cggctcatgc tgaacatgaa ctcgttgaag aaggtcggcg agaacgagtg
    11281 cgtccagttg gaggccagac tcttgtcggg aaagtagtcg gtccgggagt taccgagacc
    11341 atcgagagtg ggtacgccat tggcagccgc gcggaagcgc ttcgaatagg agttgctgag
    11401 acgagcgtac acctggtcgt tttggccgag acgctggtcg aaacgggccg tccacgtcca
    11461 ctgattgtac ttatccggcc gcgggccgaa gtagttgttc gcgactagcg ggtttacgcc
    11521 cgcctggttc ggcatcggga gctgctcggc aaagtatttc cacaaggggc tcgccagcgc
    11581 cggatcgatc gcgttggctt tgccctggta ggcaaactga ggccggaggt gcgtgaccgg
    11641 gtcggtctgg aacgggtcgt agagcctcga gagcgtgccg ctcgaatcca cgaggccgct
    11701 aaaatcgccg ttgcgcatcg cttcggtcgg gaccttgtaa cggccgattg cgccggaacg
    11761 ctgcttgaac tgctcaaaag cgaagaagaa gaaggtcctg tccttgccgt tgtatacctt
    11821 gggtatccac accgggccgc cgactgtgcc gccgtattcg ttgcggataa acttgccggg
    11881 cgtgttgccg tcctcgcgcc gacgcgccca accgtaatag ttgtcgcggt tggtctcaaa
    11941 cagcgaaccg tgtagggtgt tgccgccgcc cttggtggtc aggatgatgt tggtctggcg
    12001 gggatacttc gcggacacgg cattcatgtc caccttgaat tcctggatgc tctcgaggct
    12061 gggctgccgt ctcacggtgc cgccggcgta aaccggctcg gtgagcgcgg cgccgtccag
    12121 gctgatatcg tgagtgccgg ttcgttcgcc cagcgtgcga aggtcgccgc cctcgtcaaa
    12181 ggtcaggccg gggacggtgt tcagcaggct cataacctgc cgcccgttga tcggcaactg
    12241 ctcgatgcgc gtgcgctcga gcacatgccc cagggtctgg ctgtctgccg ttaacatcgg
    12301 agtgacgtct tccacctgca cgacggtttg agtagtttcg gggtagagga cgatattgac
    12361 ggtctcgccc ttctgcacct ggaccgtcat cgtgccttcg aacttcttca tccctgcaaa
    12421 ttcggccgtg atcttgtacg gcccgggacg aaccgcgggg aagacataga gtcctacggc
    12481 attggaggtg gtcctggatt caccgttggt cgcggtgttc gtcaggatga ccgtggcgcc
    12541 ggggatgatg gcgtcggtct ggtcctggac ggttccacgg acgctgcctg tgctcgattg
    12601 tgaccaggcc atcgaggtca ggacacatac gagtaaaaca agaacaaacg atctgtgtag
    12661 agtgttcatg atcaacttcc ctttctggac atccggagga gagccaaacc ggccggtagg
    12721 ccggagcggt ttcgcagtcg gactttaaag ctttaaagac tcccttatcc taaaagcgga
    12781 tctgcccgta gtatacaccc aatcgcccgc ggttgatcct ccgaagtgca ggtgcgcggc
    12841 tgcaatgggc aggtgcgcag gccggctggt agtggatgtg tttggccttc gcctcgacta
    12901 ctgatacact ctctccagac agggcctgca ttccggcccc agcaacgatc tcgtgacccc
    12961 atccgcttac agagtttgta gggcaagccc ccggcttgcg gcggcctctc aggccgctgt
    13021 tcctcattcg gacgtctcca acactccgac accttcattc ccgttcatcc gcgtgcattc
    13081 gcggctcact caatctatgc caatagaacg atccttcgcg gcgctgctac tctgcgccct
    13141 ctcctgttcc gcgcaaccgg cctcgggccg cgcttcaatc gacctccggt acaagctttt
    13201 ccaggagaac cttgtgcgcc gggccgaggc gatcacacgc gctttcccgg gcgacatcac
    13261 cacgctcgcc gaatggcagc gtcgccgccc ggagatcaag aagcagtact tgtacgcgct
    13321 cggactcgac ccgatgccgg cgaagacccc gctcaaagcg caggttaccg gcggcttcga
    13381 gcgtgacggc taccgcgtcg agaacatcgt ttttcagagc atgcccggtc tctacgtcac
    13441 cggcaatctc tacttgcccg cgggcaaaca gggccgtctt cccaccgtgc tctacctctg
    13501 cggtcattcg cccggacctt ggggaaacaa agtgcgctat cagcaccacg gcatctggtt
    13561 cgcccgtcac ggctacgccg cgttcctgat tgactccatc gaatttggcg aggtccccgg
    13621 actccatcac ggcacccacg acctcgaaat gtgggactgg ctatccctcg gctacacacc
    13681 cgccggaccc gaggtctgga acgccatccg cgcgctggac tacctggaaa cgcgccccga
    13741 ggtcgatccc aaacgcgtct ccgtcaccgg catctccggc ggcggcgcca tcacctggtt
    13801 cgccgccgcg gccgacgaac gcttccaggt cgctgcgccc gtctgctcca cctggaccgt
    13861 cgagcaccac accgccctcg acgccgtcc
//
LOCUS       DATIFQ010000364        10236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1461189, whole genome shotgun sequence.
ACCESSION   DATIFQ010000364 DATIFQ010000000
VERSION     DATIFQ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461189"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1018)
                     /locus_tag="VLE22_17660"
     CDS             complement(95..1018)
                     /locus_tag="VLE22_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16286.1"
                     /translation="MGTSVLVMTAMAALTVASAQTRSSTAAKSQCQDIPISYTFLSTT
                     VASAAIWNDDPTKPYENGKDGIGNALIHRCAGTNAATLALARSKRSVWIRVPNPFPNS
                     NIVNPSPSFAGGVPFQSQAFFNVRNILGSTDPYFYTSMASQFPAPDGYTHHLRLLPDA
                     GACPGDLVCIPDIHGGILPDSNLPVETSWVSIHYMPPPNPSLPWSLSNTAQWIVDGEL
                     VDSVYPASYERATLHKKGKGGNGETHEGQYSMPLQILITALGPGGEEPAKPYQSSRAP
                     TWPTRLPEFSWPCTDAVLPKLTGVSTKVSGG"
     gene            1009..1263
                     /locus_tag="VLE22_17665"
     CDS             1009..1263
                     /locus_tag="VLE22_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16287.1"
                     /translation="MFPSVFFQPVTLPPKDVGRFGNAGVGILRGPGTIAVAGGLPNHP
                     NFAAPEFFADRPGSFGKIFSVQGQENSGNRVGQVGARLDW"
     gene            complement(1365..2276)
                     /locus_tag="VLE22_17670"
     CDS             complement(1365..2276)
                     /locus_tag="VLE22_17670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB16288.1"
                     /translation="MQKTTLNDPKRTPGIAFPAMVILLLGSASMPAQTGGRRVSSSNT
                     SVLTSEPCKLDPTVALTDASVSLPSDCSPPDSLQRPPNLEHAPRTLHQGDLVSFTTLA
                     APKNAERAYEKARRNLTRKKPRVSTAARELTTAVALYPQFAAAWYLLGETRLALRQNE
                     AAREAFLRALGADPKYPSAYAPLALMELEVGRFREAAEFAGWAVSLNPDLIEAHYYRA
                     VANYNLGDTAITEESIRAIVELGGERSYPGIHLMRGQIFAAKGDFRSAEVEYRRLVQL
                     QPSSRAAHTVLQQLDDWETQGLITMPR"
     gene            2481..4451
                     /locus_tag="VLE22_17675"
     CDS             2481..4451
                     /locus_tag="VLE22_17675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB16289.1"
                     /translation="MRLPKTGTRWWWAFAVCIAAPALVLAILGVRTIDLERVEQRQGI
                     ESQQRQTALLADTTLSAALSRIQENLLRQDPDPAAGAPSFSLDDQGVLIFRRDRVYFA
                     ETGNEPPEARVAVPPAVAASVDEALAAEARAEFPRAIAIYQRIGKNPQLGAWAGLALA
                     RLRVRQQPHGFLDWIKELAPGGQDSLSPEGVPVMLLATGYVTRLPLGDAAACGPFLAD
                     VLAQLRAGRWWLSYHQRKLYDAELRTAIATIGGVAPGGDARLVEIAAVERIVRQTAPF
                     GHEGAPHPPAQAGRSGLSWTELDGYEPLLLITRERAGVALSGQRLTGFLQTRLGHIHK
                     TVPYPIAVADSSGHLLWGDATALKGKASFPLRVLFGWEFHAGEYAPGNERRQFLWYAF
                     LALLLMTLAFGLAATIRIVKRELELARLQAEFAASVTHEFKSPIAGIRLLLERITGGH
                     VLDKATIREYQHTAQRETDRLERLVNRLLETHRIQSGEKRYQFAPHCIIDIVETAVAH
                     FSPQAGAKRITVTLKTDDVLREIDLDRTSIQDSIENLLDNAIKYSPPDTNIEVSVEHG
                     AKELRVCVRDQGIGIETADLPRIFDRFYRGRRGEQQSVRGTGLGLALVKAVAEGHGGS
                     VTVKSAPGQGSEFCLCIPIREEETYVPSLDRG"
     gene            4426..5100
                     /locus_tag="VLE22_17680"
     CDS             4426..5100
                     /locus_tag="VLE22_17680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB16290.1"
                     /translation="MSRVLIVDDEPAIVIPVRDELAFEGLEVACAEDGPSGLKAALEM
                     RPDVLLLDVMLPGMNGFDICRKLRPELPEMWIILLTVRGQEVDRVTGFEAGADDYVVK
                     PFSLRELMARVRVGLRRKTPANHQRVYKFGDVEIDLRSRTVKRTGRDVPVTRKEFEIL
                     ALLARRPGEVIPRDEFCDLIWGPDVFVTQRVIDTHIASLRKKIEPEPDEPQHIFSVRG
                     VGYKLG"
     gene            5104..7116
                     /locus_tag="VLE22_17685"
     CDS             5104..7116
                     /locus_tag="VLE22_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16291.1"
                     /translation="MRRLAGILVFSMMVLGADPDIREIFERARILVARNENLAEAIRL
                     YGQVVTLARAQHVLAARAQYEQGLLYHRLGKEAEAQRAFRAVVREFPDQKGVAALARA
                     RLPAGTLETGISVRQVWTGNDPDSAGHVSPDGRLISFTDSTGDLAVHDIETGENRSVT
                     HQGSSRPGSPPPGLATWSVFSPDGRRLAYVWYGNGTELRIIGADGSGQRTLYRTTMGL
                     VAGDWSADGKYVLAFDLSKRGMLVISVADGSVRTVPVSDRWRDDSGLFSPDGNYIVYD
                     IAQEGEDPGNDIHMVSIATGRDVAVVEHRADDTMVDWSPDGSKLLFVSDRSGSPGIWA
                     IPIVDGHAAGPPELLKPDMNIQPIGSTRQGSIFYYVDVMLRDVFVAEIDPTSGKLVSP
                     PRAVSQRFVGAKDTLAWSPEGQYLIYKSPRGLSLHSMTDGKERDVQPENFGVQWIMKW
                     HPDGRSVFVSGRSGDGRFGLFRLDLQTGKAQIVARLIDRPAFSADARTLYYYAFGPTH
                     ALVAHNLDTGEVKSLYSRPDVGEIQNPNTDLSPDGKMLAFLLQNVPNGYNSLVVMPAT
                     GGEPRTLLSIQRPERFGPNSAIWTPDMRYILVSRTLNKRSTVWRVPLDGGPPQDTGLS
                     MPGLIGCLRLHPDGRRVAFFNGESHPEIWALENILPKAGTSRLARR"
     gene            complement(7205..8218)
                     /locus_tag="VLE22_17690"
     CDS             complement(7205..8218)
                     /locus_tag="VLE22_17690"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSB16292.1"
                     /translation="MRAVGLDYEHRQLLAFESAAPRLERDDQVLLRVSEVGVCGTDRA
                     LASFEFGYPPEGESHLILGHEAVCEVVETGRAVTSLRRGDVVVPSVRRACSPPCPSCA
                     RGRRDLCVSGAYRERGIFGLHGYFAEYAVDMASDLTRVPPELAGCAVLIEPLSVVEKA
                     VETALRYHEPSPETALVLGAGTIGILSALVLQARGLSVLVHSLEAPDHPRARLVASAG
                     VQYVPRLSDERSDIVIEATGSPVAALEALKRMAPLGVCCILGSPLDAEGVVPFRNLFE
                     KNQTVFGSVNASPSSFALAVEDLGRFDPNVLAGMIRRTRFSDFDETILGPQPSIPKTV
                     HVL"
     gene            complement(8225..9631)
                     /locus_tag="VLE22_17695"
     CDS             complement(8225..9631)
                     /locus_tag="VLE22_17695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB16293.1"
                     /translation="MKLSILIPVYNERTVVERSLAHVLAAPIPEAMERELIVVDDCST
                     DGTSAILDRLAASEPRIRLFRHPVNQGKGAAIRTAIQYATGDFCLIQDADLEYDPNEY
                     PALLRPLLDGHADAVFGSRYLAGQQTRVLPFRHSMINKFLTLVSNVFCDLNLTDMETC
                     YKVFRTDLLKSIPVRSNRFGFEPEIVMKSAKRKLRIYEVPISYHGRTYEEGKKIGWWD
                     GVKALGVILRFWVIDDLYIEPYGRGLLSNLTGTPQYLSWMARILRPYLGDTVLEIGAG
                     IGNISGRLMGRSVRYVAAEHDPLYLHALSNRFLRTPNVAVQCVNPASPSDFACLKGGF
                     DTAICLNVFEYLEDPMSTAAALSAVLRDGGRLLVLVPQGPGLFGSMDRTLGHLRRFHD
                     RDLRRLLEGAGFRVERVHQLNKIGMPAWWLYGNVLRRRRISKVTLKIFDKTVWFWRRV
                     DHLLPWHGLSLIAVARKA"
     gene            9710..>10236
                     /locus_tag="VLE22_17700"
     CDS             9710..>10236
                     /locus_tag="VLE22_17700"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB16294.1"
                     /translation="MSEHTSRRNFVRSSGAAAAFLIVKPESVRGSQANSALSVGLIGC
                     GGRGMYVSGLFVKNEFAKVTAVCDIYQDKLDGAVKSYSGAAQYTNHQELLASNVDAVL
                     IATPAFLHPEHFEAAVKARKHIYMEKPAGVNAAGCRRVAAAAKNADPTKRITVDYQQR
                     YGKDYRKAYEVVKSG"
BASE COUNT         1966 a         3028 c         3314 g         1928 t
ORIGIN      
        1 cggccgcggg gctgttcaag tcattctctg tcaccgaaag attgcggatg cgaatggaag
       61 tcacttacac gaatattgcg aatcatccga acttctaccc tcccgagact ttcgtcgaca
      121 ccccggtcag cttcggtaag accgcgtcag tacagggcca ggagaactcg ggcaaccgtg
      181 tgggccaggt aggagcacgg ctggattggt agggcttggc gggctcttcc ccgcccgggc
      241 ccagcgcggt aatcaaaatc tgcaacggca tcgagtactg gccttcgtga gtctcgccat
      301 tcccgccctt ccccttctta tgcagagtag cgcgctcgta ggacgccgga tacacggagt
      361 caaccagttc accatccaca atccattgcg ccgtattact caacgaccat ggcagcgacg
      421 ggtttggcgg tggcatgtaa tgtatgctca cccacgacgt ctccaccggc aggttgctgt
      481 cgggcagaat gccgccgtgt atgtcaggga tacaaactaa atctcccggg cacgcccctg
      541 catccgggag aagccgcaga tggtgtgtgt agccgtccgg ggccgggaac tggctggcca
      601 tgctggtgta gaaatacgga tctgtagatc ccaggatgtt tcgcacgttg aaaaaagcct
      661 ggctttggaa gggcacgcct cccgcaaacg acggcgacgg gttcacgatg ttggaattcg
      721 gaaagggatt agggacccgg atccagacgg aacgcttcga gcgggcgagc gccagtgtgg
      781 cggcattggt gcctgcacag cggtggatga gtgcattgcc gatgccgtct tttccattct
      841 catacggttt tgttgggtca tcgttccata ttgccgccga ggccaccgtg gtgctcaaga
      901 acgtgtaact gatcggaatg tcctggcact gacttttggc cgccgtcgag ctgcgggttt
      961 gcgccgacgc caccgtcagg gcagccattg ccgtcatcac gagaacggat gttcccatct
     1021 gtgtttttcc aaccagtaac tctgcccccc aaggacgtgg gaagattcgg aaatgccggc
     1081 gtggggattc tgcgcggacc gggtacaatc gccgtggccg gcgggctgcc caaccacccg
     1141 aatttcgccg cgcccgagtt ctttgccgac cgtccaggga gcttcggcaa gatcttttcg
     1201 gtgcagggcc aggagaactc gggcaaccgc gtgggccagg taggtgctcg actggattgg
     1261 tagttggctt gtgggttgtg gtctgtcgct tgtggcctgt ggtcgaaaga acttggctaa
     1321 tctcgcgagc ttcttccgga cctcgggcga cacttcggga cctcctacct tggcattgtg
     1381 attaggccct gcgtttccca atcgtcgagc tgttgcagga ccgtatgagc cgcgcgggag
     1441 gacggctgca attgaaccaa gcggcggtac tcaacttcgg cagaacggaa gtctcccttt
     1501 gcggcaaata tttgtccccg catcaagtga ataccggggt acgagcgttc tcctccaagc
     1561 tccacaattg cgcgaatcga ttcctctgta atcgcggtgt cgcccagatt gtagttagcc
     1621 actgcgcgat agtaatgtgc ttcgataagg tccgggttga gactgacagc ccagccggcg
     1681 aattccgcgg cctctcgaaa ccgcccgacc tccagttcca tcaaagccaa gggtgcatag
     1741 gcagacgggt acttggggtc cgctcccaga gcccgcagga aggcttctcg tgccgcctca
     1801 ttttgacgga gagccaatcg agtttctccg agcaggtacc aggcggcagc gaattgcgga
     1861 tatagcgcaa ccgccgtcgt gagttcacgt gccgcggtgg agactctcgg tttctttctt
     1921 gtcaggtttc ttcgggcctt ttcataagct cgttcagcat tcttcggtgc tgcgagcgtg
     1981 gtgaagctga cgaggtcgcc ttgatggaga gtgcgcggag catgttccag atttggcggg
     2041 cgctgtaatg aatccggcgg gctgcagtcc gatgggaggg atacgctggc atcagtcaat
     2101 gcaacggtgg ggtccagttt gcagggctca gaggttaaca cagaagtgtt acttgagctt
     2161 actcgccgcc ctccggtttg tgccggcata gatgcgcttc ccaacagcag gataaccatt
     2221 gccgggaacg caattccggg agttcttttc gggtcgttca acgtagtctt ttgcattacc
     2281 cctccttcca gaacagccac agtctagccg catcaccgct ggcaagtcgt tagggcccgg
     2341 tcaagatttc gtcatgtttc tgtatgggca ggagcctggt gtatcttgac ggaaatttga
     2401 cactccacgg ctgagaaact cggttacact ccttagaagc aggctacttt gacgaaagtg
     2461 gccgacaaag gcgccgggcg atgaggcttc cgaagacggg tacgcggtgg tggtgggcgt
     2521 tcgccgtgtg catcgcggcg ccggcactgg tgctggcgat tctcggggta cggaccatcg
     2581 atctggaacg ggtcgagcag cggcaaggga tcgagtccca acagcgccag accgcgttgc
     2641 tggcggatac caccctgtcg gcggcgctgt cccgcatcca agaaaacctg ctccggcagg
     2701 atccggaccc agcggccggc gcaccgtcgt tctctctgga tgaccaaggc gtgctgattt
     2761 ttcgccgaga tcgagtctac ttcgcggaga ccggcaatga gccacccgag gcccgcgttg
     2821 ccgttccgcc agcggtggcg gcctccgtgg acgaggcttt ggccgccgag gcgcgggcgg
     2881 agtttccgcg cgccatcgcg atctatcagc gcatcggcaa aaatccccaa ctgggggctt
     2941 gggccggact cgcgctggcg cggctccggg ttcgccagca accacacggt tttctcgact
     3001 ggatcaagga actggcgccc ggcggccagg attcgctttc cccggagggt gtgccggtga
     3061 tgctcctcgc cacaggctat gtaacccggc tgcccctggg ggatgccgcg gcttgcggcc
     3121 cattcctcgc tgatgtgttg gcgcagttgc gcgccgggcg ctggtggctc agctatcacc
     3181 agagaaagct gtacgacgcc gaactgcgaa ctgcaatcgc gaccataggg ggcgttgccc
     3241 caggggggga cgcgcgactg gtcgaaatag ccgccgtcga gcgcattgtg cgccaaaccg
     3301 ctcccttcgg ccacgagggc gcacctcacc cacccgcaca ggctggcagg tcagggctgt
     3361 cttggacgga attagatggg tatgagccac tccttctgat cacacgagaa cgggccggag
     3421 tcgcgctttc tggccagcgg ttgaccggct tcttgcagac caggctgggc cacatccaca
     3481 agaccgtgcc ctacccgatc gctgtggcgg actcctccgg gcacctcttg tggggcgatg
     3541 ccacagccct caaggggaaa gcctcctttc cgctccgagt tctatttggg tgggagttcc
     3601 atgccggcga atacgcacca ggcaacgaaa gacgccagtt tctttggtat gcctttctgg
     3661 cgcttctgct gatgacactg gcgtttggat tagcagcgac gatccgaatt gtgaagcgcg
     3721 agctggaact ggccaggcta caggctgagt tcgccgccag cgtgacccac gagttcaaga
     3781 gtcccatcgc gggcattcgt ctcctgctgg agcgaatcac cggcgggcac gtgctcgaca
     3841 aagcgaccat ccgcgaatac cagcacactg cccagaggga aaccgatcgt ttggagcgcc
     3901 ttgtcaaccg gctgctcgaa acacaccgga ttcagtccgg ggaaaagcgc tatcagttcg
     3961 ctccgcattg catcatcgac atcgtggaga ctgcggttgc ccacttcagt ccacaagctg
     4021 gagccaagcg catcacggtg acattgaaga ctgacgatgt gctgcgcgag atcgacctgg
     4081 atcggacgtc gattcaggat tcgatcgaga atctgcttga caatgccatc aaatactcgc
     4141 cgccggacac gaatatcgag gtctcggtgg agcatggtgc gaaggagctg cgcgtctgtg
     4201 tccgcgatca gggcattgga atcgagacgg ccgatctgcc gcgcatcttc gaccggttct
     4261 atcggggacg gcgcggcgag cagcaatcgg tgcggggtac aggccttggc ctcgcgctgg
     4321 tcaaggcggt ggcggaaggc cacgggggtt cggtgacggt gaagagcgcg cccgggcagg
     4381 gatccgagtt ttgcctgtgc atcccgatcc gggaggagga aacctatgtc ccgagtcttg
     4441 atcgtggatg acgagcctgc gatcgtcatt cccgtccgcg acgaactggc cttcgagggc
     4501 ctggaggtcg cgtgcgcgga ggacgggccg agcgggctaa aggccgcgct cgagatgcgg
     4561 ccggatgtcc tgctgctcga tgtgatgctg ccgggcatga acgggtttga catttgccgc
     4621 aagctgcgcc ccgagctgcc cgagatgtgg atcattctgc tgaccgtgcg cgggcaggag
     4681 gtggatcggg tgacaggctt cgaggcggga gcagacgact acgtggtgaa gcccttctca
     4741 ctgcgcgagc tgatggcgcg ggtccgcgtg gggttgcgac gcaagacccc tgctaaccat
     4801 cagcgggtct acaaattcgg cgatgtcgag atcgacctgc gctcgcgcac cgtcaagagg
     4861 accggccgtg acgttccggt gacgcgcaaa gaatttgaga tcctggcgct gctggcgcgc
     4921 cgcccgggtg aagtgatccc gcgcgacgag ttctgcgatc tcatttgggg gccggatgta
     4981 ttcgtcaccc agcgggtgat cgacactcac atcgcgtccc tgcgaaagaa gatcgagccg
     5041 gagcccgacg aaccacagca catcttcagc gtgcgcggcg tcggatacaa gctggggtaa
     5101 gctatgcgac gccttgccgg gatcctcgtc ttcagcatga tggtgttggg cgccgatccg
     5161 gacatccgcg agattttcga gcgtgcccgc atcctcgtgg cgagaaacga gaacctggcc
     5221 gaagccatac gactctatgg ccaagtcgtg actctggccc gggcccagca tgtgctggcg
     5281 gcacgggcgc agtacgagca ggggctgctc tatcaccgtc tcggcaagga ggcggaagcc
     5341 cagcgggcgt tccgcgctgt tgttcgcgaa ttcccggacc agaaaggagt cgcggctttg
     5401 gcgcgagcaa ggctgccggc gggcactctg gaaacgggga tatccgtgcg gcaggtgtgg
     5461 acggggaatg acccggactc cgccgggcat gtgtcgcccg acggacggct gatctctttt
     5521 acggattcca ccggggatct ggcggttcac gatatcgaga cgggtgagaa ccggagcgtg
     5581 acccaccagg gcagttctcg gccggggtcg ccgccgccgg gcctcgcaac gtggtccgtt
     5641 ttttcgccgg acggtcggcg gctggcctat gtctggtacg ggaacggcac cgagctgcgg
     5701 atcatcggcg cagacggatc ggggcagaga accctgtatc gaaccacgat gggtttagtg
     5761 gccggtgact ggtccgcaga tgggaaatac gtgctggcct tcgaccttag caagcgaggc
     5821 atgctggtga tttcggtggc ggacggctcc gttcgtacag tccctgtctc cgaccgctgg
     5881 cgggacgaca gcggcctgtt ctcgccggat ggcaactaca ttgtctacga catagcgcag
     5941 gagggcgagg accccggcaa cgacattcac atggtatcga tcgcgacggg gcgggatgtg
     6001 gcagtggtgg aacaccgggc cgacgatacc atggtggact ggtcgccgga cggttcgaag
     6061 ctcctgtttg tgagcgatcg gtcgggatcg ccgggcattt gggcgattcc catcgtggac
     6121 gggcacgcag cggggccgcc tgaactgttg aaaccggaca tgaatatcca gccaatcgga
     6181 agcacccgcc aaggctctat cttctactac gtcgatgtga tgttgaggga tgtgtttgtg
     6241 gctgaaatcg accccacttc gggcaagctg gtgtcaccgc cgagggcggt cagccagcgg
     6301 ttcgtgggcg ccaaggacac cctagcctgg tcgcccgagg gccagtacct gatttacaag
     6361 tccccacgag gcctgtcgct acattccatg acggacggaa aggagcggga tgtgcagccg
     6421 gagaactttg gggtccagtg gatcatgaag tggcacccgg acggccgttc cgtatttgtc
     6481 agtggcagat cgggtgacgg acggttcgga ctgttccggt tggacctcca aaccgggaag
     6541 gcgcagatag tcgcccggct gatcgatcgc ccggcgttct ccgcggatgc ccgcacactc
     6601 tattactacg cgtttggtcc aacgcatgcg ttagtcgccc acaacctcga taccggcgaa
     6661 gtgaagtcgc tctactcccg gccggacgta ggcgaaatcc aaaacccaaa cacggacctt
     6721 tctcccgacg gcaagatgct tgctttcctg ctgcagaacg ttccgaacgg gtacaactcg
     6781 cttgtcgtga tgccggcgac gggtggcgag ccgcgcacgc ttctttcgat ccaaaggccg
     6841 gagcgctttg gccctaactc ggcgatctgg actccggata tgcgttatat ccttgtctcg
     6901 cgaacgttga acaagaggtc gacggtctgg agggtaccgc tggacggagg accgcctcaa
     6961 gataccggcc tgtccatgcc tgggttaatc ggctgtctcc ggctgcaccc ggacgggcgg
     7021 cgggtggcgt ttttcaacgg cgagtcacac ccggagattt gggcgctgga gaacatcctg
     7081 cccaaggcgg gcacgagccg gctggcgagg aggtgaattg gcgagcgcca ttgaaacagc
     7141 attaacgaat ggttcagcca cgtttcttga aaccggcggc gagagcgcat gcggcatgcg
     7201 agtgttacag tacgtggacg gttttcggga ttgaaggctg gggtccgagg atcgtttcgt
     7261 caaagtccga gaagcgcgtg cggcggatca tgccggcgag cacgttcggg tcaaatcgtc
     7321 cgaggtcttc gaccgccagc gcgaacgacg acgggcttgc attgacgctg ccgaagaccg
     7381 tctggttctt ctcgaagagg ttgcgaaacg ggacgacgcc ctcggcgtcg agcggcgagc
     7441 cgaggataca gcagacgccg agcggggcca tccgcttcag cgcctccagg gcggctaccg
     7501 gcgagcccgt ggcctcgatc acgatgtcgg acctttcatc ggagagtctt gggacgtact
     7561 gcacaccggc ggaagccacc agccgcgccc gggggtggtc cggagcttcc agcgaatgca
     7621 caaggaccga cagcccgcgc gcttgaagca cgagggcgct gaggatgcca atggttcccg
     7681 ctccgagcac gagggcagtc tcgggcgagg gctcatggta gcgcaaggcg gtttcgacgg
     7741 ctttctcgac gacgctcagc ggctcgatca gcacggcgca accggcgagc tcgggcggca
     7801 ccctggtcag gtcggaggcc atatcgaccg catattccgc aaagtaaccg tgaagtccga
     7861 agatcccccg ctcgcggtac gcgccgctga cacacaggtc gcgccgccca cgcgcgcagg
     7921 agggacacgg cggactgcaa gcacggcgga ccgaaggcac cactacgtca ccccgacgaa
     7981 gcgaggtcac cgcccggccc gtctccacga cctcgcaaac ggcctcatgg ccgagaatca
     8041 ggtgactctc accttctggc ggatagccga actcaaaaga agctagcgcg cggtccgtac
     8101 cgcagacgcc cacctcgctc acgcgcaaca gaacctggtc gtcgcgctca aggcgcggag
     8161 cggcggattc aaatgccaaa agctgccgat gttcgtagtc gaggccgacc gctcgcatgg
     8221 attactacgc ctttcgggcc accgcgatca gagacaatcc gtgccagggc agcaggtggt
     8281 ccacgcggcg ccagaaccag accgtcttat cgaagatctt gagggtgacc ttgctgatgc
     8341 gacgccggcg cagtacgttt ccgtacagcc accatgcggg catgccgatc ttgttgagct
     8401 ggtgcacgcg ctcgacgcga aagccggcgc cttccaggag cctccgcaag tcgcgatcat
     8461 ggaaccggcg gaggtgaccg agcgttcggt ccatcgagcc gaagaggccg ggcccctgag
     8521 gcaccaggac gagcaggcgt ccaccgtccc ggagcaccgc gctcagggcc gccgccgtcg
     8581 acatggggtc ctcaaggtac tcgaacacat tgagacagat cgccgtgtca aagccacctt
     8641 tcagacacgc aaaatcgctg ggggaagccg gattcacgca ctggaccgcc acgttcggcg
     8701 tgcgcaggaa ccggttgctc aaggcgtgga gatacaacgg atcgtgttcg gccgccacat
     8761 accgcacgct ccggcccatg aggcggccgc tgatgttacc gatgccggcc ccgatctcaa
     8821 ggacggtatc gcccagataa ggccgcagga tccgcgccat ccaactcagg tactgcggcg
     8881 ttccggtgag gttgctgagc aatccgcgcc cgtacggctc gatgtacaga tcgtctatga
     8941 cccagaaacg cagaatcacg ccaagggcct ttacgccatc ccaccagccg atctttttgc
     9001 cttcctcgta ggttctgccg tgatagctga tcgggacctc gtagatgcgc agcttgcgct
     9061 tggcggactt catgacgatt tccggctcaa agccgaagcg gttcgagcgc acggggatgc
     9121 tcttgagcag atcggtccgg aagaccttgt aacaggtttc catgtccgtc agattgaggt
     9181 cgcagaagac gttcgagacc agcgtgagaa acttgttgat catcgagtgc cggaacggca
     9241 gcactcgggt ctgttgtccg gccagatagc gtgagccgaa caccgcgtcg gcatgcccgt
     9301 cgagcagcgg gcggagaagc gcggggtact cgttcggatc gtactcgagg tcggcatcct
     9361 gaatcagaca gaagtctccg gtggcgtact ggatcgcggt ccggatcgcg gcgcccttac
     9421 cctggttgac cggatgccgg aacaaccgga tacgaggctc ggaggcggcg agccggtcga
     9481 ggatggcgga ggtcccgtcg gtcgagcaat cgtccacaac gatgagttcg cgctccattg
     9541 cctcgggaat aggcgccgcg agaacgtggg caaggctgcg ctcaactacc gtccgttcgt
     9601 tgtagacggg aatcaggatt gagagtttca ccggagattc cccagtataa tgggtcggga
     9661 agcgacataa cccgtccgtt ccagatcccg tttattccag gaggctctca tgagtgagca
     9721 cacaagccgg aggaatttcg tacggtcgtc cggcgccgcc gcggcgtttc tgattgtgaa
     9781 gccggaatcc gtccggggct cacaggccaa ctcagcgctg tcagttggct tgatcggctg
     9841 cgggggccgc ggcatgtacg tcagcggcct gttcgtgaag aacgaattcg caaaggtgac
     9901 ggccgtctgt gacatctatc aggacaaatt ggacggcgcg gtgaagagct attccggcgc
     9961 cgcgcagtac acgaaccacc aggaactgct cgcgagcaac gtcgacgcgg tcctcatcgc
    10021 cacgcccgcg ttcctgcacc cagaacactt tgaagcggca gtgaaggcgc ggaagcacat
    10081 atacatggaa aagccggcgg gcgtgaacgc cgccggctgc cggcgcgtgg ccgcagcggc
    10141 gaagaacgca gacccgacca agcgcattac ggtcgactac cagcagcgct acggcaagga
    10201 ctaccgtaag gcgtacgagg tggtgaaatc cggcga
//
LOCUS       DATIFQ010000365         2434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_696722, whole genome shotgun sequence.
ACCESSION   DATIFQ010000365 DATIFQ010000000
VERSION     DATIFQ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696722"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VLE22_17705"
     CDS             complement(<1..356)
                     /locus_tag="VLE22_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16295.1"
                     /translation="MEVLEEGAFGTRFIAREGEEDGGLDDVVTEDEGEAAAVVKVRVL
                     PFHALAEGVEAELEEAGLDGADAGEAPGGDGELFDEEGLGRGGGLVFVDEGLMEGLVG
                     GAVLDVDERVLGGEAVA"
     gene            complement(516..1919)
                     /gene="uxaC"
                     /locus_tag="VLE22_17710"
     CDS             complement(516..1919)
                     /gene="uxaC"
                     /locus_tag="VLE22_17710"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HSB16296.1"
                     /translation="MSFIHEDFLLTTATARRLYHEFAAAEPILDYHCHLSPKDIAENR
                     RFANLFEIWLEGDHYKWRAMRANGVDERYCTGDASPWKKFLAWARTVPHTLRNPLYHW
                     THLELKRYFGIEDLLDEYTAPAVWERANCMLGGDDLTAQGILRKFNVTALCTTDDPSD
                     DLVHHKAIAESRFTTRVFPAFRPDKALNVHLPEVFNAWVDRLESAADMNIATLPALVD
                     SLRRRHDYFHEAGCRLSDHGLNHCYADFCTDRQAAKVFDKARGGSPATPEEHARFASF
                     MMLIFGRLDAEKGWTKQLHLGARRGANTRMLKELGPDTGYDSIGDWPQIDALGAYLDR
                     LDRENALPKTIVYNLNPADNYACATMIGNFQGGGIPGKIQLGSGWWFLDQKEAMEWQI
                     NALSNAGLLSRFAGMLTDSRSFMSYPRHEYFRRVLCNLLGRDVENGELPDRAELLGPL
                     IRGICYGNARAYLNLPL"
     gene            complement(1933..>2434)
                     /locus_tag="VLE22_17715"
     CDS             complement(1933..>2434)
                     /locus_tag="VLE22_17715"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HSB16297.1"
                     /translation="ASLKLSVHSGSDKFSIYAPIRRSLAKFGAGVHVKTAGTTWLEEL
                     IGLAEAGGDGLALARNVYVKAYAAREELCAPYASVIDVDPAKLPAPETVAAWSSEQYV
                     AALRHDPRCSDFNPHLRQLLHVGYKVAAKMGDRYYGMLDACAEPIARNVITNLFDRHI
                     KPIFLG"
BASE COUNT          435 a          770 c          784 g          445 t
ORIGIN      
        1 gccacggcct cgccgcccag aacccgctca tcaacatcga ggaccgcgcc gcctaccagg
       61 ccctccatca ggccctcatc gacgaacacc agccctccac ccctacccag tccctcctcg
      121 tcgaacagct cgccatcgcc tcctggcgcc tcgcccgcat ccgctccatc gaggccggcc
      181 tcttcgagtt ccgcctctac accctccgca agagcatgaa aagggagcac tcggaccttg
      241 acgaccgcgg ccgcctcgcc ttcgtcttcc gttacgacgt cgtccagacc accatcctcc
      301 tcaccctctc gcgctatgaa tctcgtgccg aacgctcctt cttcaagaac ctccacgagc
      361 ttcagcgcct gaaggctgcc gcccccggcc cggtgtccga ttccgggatt ggctttgttc
      421 agtcaacggt ccccgacacg actgacccca gtccggacga cgaaccgaac gacgccccct
      481 ccgctcccaa ctcgcagggg agtgactctc cctcattaga gcgggaggtt gaggtaggcg
      541 cgggcgttgc cgtagcagat gccgcggatg agcgggccga ggagctcggc gcggtccggc
      601 agttcgccgt tctcgacgtc cctaccgagc aggttgcaga gaacgcggcg gaagtactcg
      661 tgccggggat aggacatgaa cgaacgggaa tcggtgagca tgccggcgaa acgtgacagc
      721 agccctgcat tcgacagggc gttgatctgc cactccatcg cttccttctg atcgagaaac
      781 caccagccgc tgccgagctg gatcttcccg ggaatgccgc ctccctgaaa gttgccgatc
      841 atcgtggcgc atgcgtagtt atcggctgga ttcaggttgt agacgatcgt tttcggcagg
      901 gcgttttccc gatcgaggcg gtcgagatag gcgccgagag cgtcgatctg aggccagtcg
      961 ccgatggagt cgtagccggt gtccggaccg agttccttca gcatccgggt gttcgcgccg
     1021 cggcgcgcgc ctaggtgaag ctgcttggtc cagcctttct cggcgtcgag ccggccgaag
     1081 atgagcatca tgaaagacgc aaaccgcgcg tgctcttccg gtgtcgcggg actgccccct
     1141 cgggccttgt cgaagacctt ggcggcttgg cggtccgtgc agaaatccgc gtaacagtga
     1201 ttcaggccgt gatcggagag gcggcagccc gcctcgtgaa agtaatcgtg ccggcggcgg
     1261 agcgagtcga cgagcgcggg cagcgtggcg atgttcatgt ccgcggcgct ttcgaggcgg
     1321 tccacccagg cgttgaagac ctctggcagg tggacgttca aagccttgtc cggacgaaag
     1381 gcgggaaaga cgcgcgtcgt gaaccgcgac tcggcgatgg ccttgtggtg gacgaggtcg
     1441 tcgctcggat cgtcagtcgt gcacagcgcg gtcacattga acttacggag gatgccctgg
     1501 gcggtcagat cgtcgcctcc gagcatgcag ttggctcgct cccagacggc gggtgcggtg
     1561 tactcgtcga gcaggtcttc gatgccgaag tagcgtttca gctcgaggtg agtccagtga
     1621 taaagcggat tgcgtagggt gtgcggaaca gtccgtgccc acgccagaaa cttcttccac
     1681 ggcgaagcgt cgccggtgca gtagcgttcg tccacgccgt tcgcgcgcat cgcccgccat
     1741 ttgtagtggt cgccctcgag ccagatctcg aacaggttgg cgaaccgacg gttctccgcg
     1801 atgtccttcg gcgaaaggtg gcaatggtag tcgagaatcg gctcagccgc cgcgaactcg
     1861 tgatagagcc gccgggcagt cgcggtagtg agcaggaaat cctcgtggat gaaggacatg
     1921 acgttacctc gctcaaccga ggaagatcgg tttgatgtgc cggtcgaaga ggttggtgat
     1981 cacgttccgc gcgatcggtt ccgcgcaggc atccagcatg ccatagtaac ggtcacccat
     2041 ctttgcggcc accttgtagc cgacatggag gagctggcgg agatgcggat tgaagtccga
     2101 acagcgggga tcgtgccgaa gcgccgcgac gtattgctcc gacgaccatg ccgcgacggt
     2161 ctcgggtgcg ggcagcttcg ccgggtcgac gtcgatgacc gaggcatagg gagcgcaaag
     2221 ctcctcgcgg gcggcgtaag ccttgacgta aacgttgcgg gcgagcgcta ggccatcgcc
     2281 gccggcttcg gcaagcccga tcaattcttc aagccaggtg gtgcccgcgg tcttgacgtg
     2341 cacgccggcg ccgaacttcg cgagcgagcg gcggatgggg gcgtagatcg aaaacttgtc
     2401 gctgccggag tggacactga gcttcaggct ggct
//
LOCUS       DATIFQ010000366         7608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_887856, whole genome shotgun sequence.
ACCESSION   DATIFQ010000366 DATIFQ010000000
VERSION     DATIFQ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887856"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VLE22_17720"
     CDS             complement(<1..273)
                     /locus_tag="VLE22_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16298.1"
                     /translation="MDSRVVNKLIRSEVWPLLRDHGFSRFESRTAFCYRGPFINVVNF
                     QSFNAYLAVGVGCTTFSFGLNLGVYVMGSAREHLLKRDASGLLLPRE"
     gene            complement(316..924)
                     /locus_tag="VLE22_17725"
     CDS             complement(316..924)
                     /locus_tag="VLE22_17725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSB16299.1"
                     /translation="MLAILEPLAVFGLIIAYIWGLRTMYPDLWIAILGLMLLSHLLRG
                     ESPGMLGFGTRNLGAVMRELAPLLAFVALLMLGCGILLRTIRPISIDGAVVALAGYLP
                     WGLLQQYILNGYFLNRFDAVFSRRAATMITATLFCAAHAPNWLLMGVAFLGGYWATRV
                     YQRHRNLYVMGLAHAIAGFLLFLVVPDSISHHLRVGPAWFGG"
     gene            complement(940..1824)
                     /locus_tag="VLE22_17730"
     CDS             complement(940..1824)
                     /locus_tag="VLE22_17730"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HSB16300.1"
                     /translation="MGQPTVLVLLLAAVVAFFAPGLQSRVQSALHRRPILIWATPFFL
                     TVVFNGASWLAGAFSVPLSLLVLAYTAVPVLCAWVQGPGPIQRPTILDFVTILLLWLP
                     LEFAAGASLLPRAAQGFLHSVAYGIAILLGLVLFLSFRSWPGLKYNLPRWKSDFLLPL
                     AGFAISAPVLMVVGLALGFIPPPHLPAQTAARMAGAAGLIFAGTALPEEILFRSLIQN
                     GMMLRFGDNIRTLLAASFLFGCAHLDNGPQPLPNWRYMTLATIAGVAYGKVFQRSSTV
                     LSSSGLHMLVNWTKHFFF"
     gene            2129..3286
                     /locus_tag="VLE22_17735"
     CDS             2129..3286
                     /locus_tag="VLE22_17735"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB16301.1"
                     /translation="MKIIRIEAVTFRKDLRIGGGSGGSGTTEFFWVRLHTDRGIVGTG
                     ETYPFSAGEIGALKDYSRQILGRDARDIDGIWRDFYHAMAMRNAGGADMRILSAINMA
                     QLDILGQAAGLPLYRLLGGKTRERVRVYNTTTDYWAINEMKMGRDTMKIVGFLLERGI
                     SAIKIYPFSARDSYLSNQALENGLQWIQEIRDKTGNRMDICVDCWGRFDFPSAVRIAK
                     ALEPYNIMYLEDAMLMNNAQSYARLAAETSVAVCMSETLATRYQYREFLEQKACDVVM
                     YDLTWCGGVTEAKKIADMADTYFIPTSPHTCGGPLLYLCSTHLSLAVPNFLIMESNYW
                     KYTHQFPHFVNNVPVPKDGHVIPPELPGIGAEIKPELFRNGDAVVETVAEV"
     gene            3438..4061
                     /locus_tag="VLE22_17740"
     CDS             3438..4061
                     /locus_tag="VLE22_17740"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HSB16302.1"
                     /translation="MSILEIKQENGPGSLSRRALFSILPAFGCLGCARAMACTQPSQA
                     APPRPSWTEKADVTWEEMFRFAYQKDLIPVLKELGAQLGREEFVGMLRGASDEVVRKK
                     TAGRPPAVRDLVTFAASMKNMPPLFQHALEAEIVEQSPEAFEYRVRKCLWAKVFRDED
                     AADIGYAMVCYPDYAVAKGLNPKLQLIRTKTLMQGDDSCSLRYVMQA"
     gene            complement(4377..4853)
                     /locus_tag="VLE22_17745"
     CDS             complement(4377..4853)
                     /locus_tag="VLE22_17745"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB16303.1"
                     /translation="MQQSTTRVNPSEETIKIGPLGIRFLLTGDDSNGSVSVFEVVVPV
                     GQKLAAPAHKNDAYEEILYGIEGVLTWTVDGTPIEVGPGQALCIPRGAVHRFDNLGSE
                     DVKQLAVITPAIMGPAYFREAAEVINAAAGGPPDRAKMTDVFRRHGMTVAAPPPDK"
     gene            complement(5045..6688)
                     /locus_tag="VLE22_17750"
     CDS             complement(5045..6688)
                     /locus_tag="VLE22_17750"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HSB16304.1"
                     /translation="MRDGACHNDSTKRKYKLWVIGAGFVLLILGVAGLITARILSRRF
                     EPYVREQAVEYLKKRFASEVELRALHVRLPRTSPLRLLMKRGRGVITHVDGEGLTMRL
                     RSRPELPPIFAIRKFAFEIDLGKLFDATRTVPLVVLDGMEINVPPRSQQPAKRAGGAP
                     ESKGASPAPDGSLPSVLIEKVVISKAVLVTLPRDPKKVPLRFDIHHLELESAGPGVPM
                     NYEARLTNAKPPGEIVSKGTFGPWAAGEPGDSPLAGEYVFEKADLGVFAGIAGILTST
                     GNFQGTLAAIEARGEAKVPDFRLKMANNPVPLTTKFEVLVDGTNGNTVLRPVHATLGS
                     TSFLTSGAVIRHEGEQRRTVSLNVSMPDGAIRDLLRLAAKGPPFMEGRVMLDTRIDIP
                     PLSGKVREKLILDGRFELLEGKFLRTTIQDQIDNLSRRGQGAPTNTDIDEVASYMTGE
                     FRLENEALSFRSLAFGVPGAHVQLAGGYNLANDALDLHGNLRLQSKVSGTVTGWKRWA
                     LKPVDPFFAKRGAGTFLNIKVDGTSQHPKFGLDRNGGRK"
     gene            6767..7582
                     /locus_tag="VLE22_17755"
     CDS             6767..7582
                     /locus_tag="VLE22_17755"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB16305.1"
                     /translation="MKPALLLAFLFIPAVPQTQDSTPVRRARTVLEQGAADPDPDVRR
                     EVAVALGLPSRRDPSTNLLAKLAADKDHLVREAALVSIGELRDPRLAKPAQDALEDEV
                     PEVAFAAARTLFKLNQPAGKQLLIEIVQREARGKTGFVRAKLRDAARHMSRPKSAMLF
                     VAQQGVGFVPVPGLGGGFSAMGSLVGDPDFSARATALIVLSSDRSPEVRSQIEQAFND
                     SDWSMRAAATQIAASRNERSWRFRLVPLFEDTNRRVRYRAAASFLRLSGPAAR"
BASE COUNT         1560 a         2276 c         2404 g         1368 t
ORIGIN      
        1 ttcccgcggg aggagcaggc cgctggcatc cctcttgagg agatgctctc gggcgctccc
       61 catcacatag actccgaggt ttagaccaaa ggaaaacgtc gtgcagccga cgcccacggc
      121 gaggtaggcg ttgaacgact ggaaattgac aacgtttatg aacggtcccc gataacagaa
      181 cgccgtacgg gactcgaatc ttgaaaagcc atggtcccga agaagtggcc aaacctcgga
      241 tcgaattagc ttgttgacaa ccctgctatc catcgtccaa caattatccg tcggatatcg
      301 cgctcgatga agtagttatc cgccaaacca ggccggcccg acgcggaggt gatgactaat
      361 cgaatccggt accactagaa agagcaggaa tcccgcgatg gcgtgggcca ggcccatgac
      421 atagaggttt cggtgccgct gatatacgcg tgtggcccaa taaccaccca ggaatgcgac
      481 gcccatcaag agccagttcg gggcatgggc ggcgcagaaa agcgtcgccg tgatcatggt
      541 cgccgcgcgt cgggagaaca ccgcgtcgaa gcggttcagg aagtagccgt tcaaaatgta
      601 ctgctgcaac aggccccacg gcagatagcc cgctagcgcc acgacggccc cgtcaatact
      661 aatcggccgg atggtgcgaa gcagtatgcc gcaacccagc atcagaagcg ccacaaacgc
      721 aagcagcggt gcaagctcgc gcatcacagc tcccaggttg cgcgtcccga agcccagcat
      781 gccggggctt tcgccacgga gcagatgcga tagcagcatt agccccagga tcgctatcca
      841 caggtcgggg tacatagtgc ggagtcccca gatgtacgca atgatcaggc cgaagacagc
      901 gagcggttcc agaatggcga gcatgcgatg cacggtcact caaaagaaga aatgtttcgt
      961 ccagttcacc agcatgtgca agccgctgga ggaaaggacc gtcgaggatc tttgaaagac
     1021 cttgccgtag gcgacgccgg cgatggtcgc cagggtcatg taccgccaat tcggcagagg
     1081 ctgcgggccg ttgtcgagat gcgcacagcc gaagaggaaa ctggcggcta gaagcgtgcg
     1141 aatgttgtcg ccaaaccgca gcatcatccc gttctggatg agcgagcgga agaggatttc
     1201 ttccggcagc gcggttcccg caaagatcag accggccgct ccggccatcc gagcggccgt
     1261 ctgggccggg agatgcggcg gcgggatgaa gccgagcgcc agccccacaa ccatgaggac
     1321 tggcgccgag atcgcaaaac cggcaagcgg cagcaagaaa tcgctcttcc agcggggcaa
     1381 gttgtatttc aatccgggcc aggaccggaa gctgaggaac agcaccaggc cgagaagaat
     1441 ggctatgccg taagcaacgc tgtggaggaa tccttgcgcc gctcgtggga gaaggctggc
     1501 gccggcggca aactcgagcg gcagccaaag cagcagaatg gtgacgaaat cgagtatcgt
     1561 cgggcgttgg atgggtcccg gaccttgcac ccacgcgcac aggacgggta ctgcggtgta
     1621 ggccaacacc aggagactca gggggacgct gaaggctccg gcgagccatg acgcgccgtt
     1681 aaaaacgacg gtgaggaaaa acggggtggc ccaaatcagg atcggccgcc ggtggagtgc
     1741 gctctgcacg cggctctgga gacccggcgc gaagaaggcg accacggcgg cgagtaatag
     1801 aacaagaacg gttggttgcc ccatgagctt attgcatcac ctgccaggct cgatctgcac
     1861 tgtccaggcg atgtgatatg cttgcgcacg cgtcaggaaa agattctctc cgggcatgtg
     1921 gaaggagagg atgtggcatg gctggcccca caggtcgtcc agaggctctg cgctgctgag
     1981 aactccaaat caggaggtgg gaagcatgac aagcggtggc aggacccgga ggagctttct
     2041 cgcccagggc gttaacgcgc cgcttaccgc aatctccacg agccctgtct tcgccgcact
     2101 gcagacggcg gcaggtagaa cggacgtgat gaagatcatc agaatcgaag ccgtcacatt
     2161 ccggaaggat cttcggatcg ggggcggatc gggcggatca gggacgaccg agttcttctg
     2221 ggtgaggctg cacaccgaca ggggcatcgt ggggacgggt gagacctatc ccttttcggc
     2281 tggagaaatc ggagcgctca aggattactc gcggcagatc ctggggcgcg acgcacgcga
     2341 catcgatggg atttggcgcg atttctacca cgccatggcc atgcggaacg caggcggcgc
     2401 ggacatgcgc atcctgagcg caatcaacat ggcccagttg gacattctgg gtcaggcagc
     2461 gggcctgcca ctctaccgtc tcctgggcgg aaagacgcgc gaacgggtca gggtgtacaa
     2521 cacgacgacc gattactggg ccatcaacga aatgaagatg ggccgcgaca cgatgaagat
     2581 cgtcggattc ttgctggagc ggggcatctc ggcgatcaaa atctacccgt tcagcgcgcg
     2641 cgacagctac ctgtcaaatc aggcactcga gaatggcctg caatggatcc aggagatccg
     2701 ggacaagacg gggaaccgca tggatatctg cgtggactgc tgggggcgct tcgacttccc
     2761 aagcgcagtg cggattgcca aggcgctgga gccgtacaac atcatgtacc tcgaggacgc
     2821 catgctgatg aacaacgcac agtcctacgc gcggctggcc gccgagacca gcgtcgcggt
     2881 ctgcatgagc gaaacgctgg cgacgcgcta tcaataccgc gagtttctcg agcagaaggc
     2941 ttgcgacgtg gtgatgtatg acctaacctg gtgcggcggc gtcacggagg caaagaagat
     3001 cgccgacatg gcggatacct atttcatccc gacctcacct catacctgcg gtgggccgct
     3061 gctctacctg tgttcaactc acctttccct ggcggtgccg aacttcctca tcatggagag
     3121 caactactgg aaatacacgc accagttccc gcacttcgtc aacaatgtgc ccgtccccaa
     3181 agacgggcat gtcatccctc ccgagttgcc aggcatcggt gcggagatca agcccgagtt
     3241 gttccgcaat ggcgacgcgg tagtggagac cgtggcggag gtgtaatttc ccctcttgta
     3301 tcaaactcgc gcttcagcgg ggaaccgcga gttggacgcc ggtgtccaac ctagcgaaag
     3361 ccgggcgcac ggccgcagag agacggccat tacggcgagc ttgttgggtg ctggtcgcgg
     3421 cgcggaggcc gcatcgtatg agcattctgg agatcaagca agaaaatggg ccgggttcac
     3481 tcagccggcg cgcactgttt tccattcttc cggcgttcgg atgcctgggc tgtgcgcggg
     3541 caatggcctg cacacaacct tcgcaggcgg cgccgccccg tcccagttgg acggaaaagg
     3601 cggatgtgac gtgggaggag atgttccgct tcgcttatca gaaagacctg attcccgtgc
     3661 ttaaggaatt gggagcgcaa ctcgggcgtg aggagttcgt tgggatgttg cgaggggcct
     3721 ccgacgaggt ggtgcgaaag aagactgccg ggcggccgcc ggcggttcgc gacctggtga
     3781 ccttcgctgc cagcatgaag aacatgccgc ccctgttcca gcacgcgctg gaagccgaga
     3841 ttgtggagca gtctccggag gcgttcgagt accgcgtgag gaagtgctta tgggccaagg
     3901 tgtttcgcga cgaggatgcc gccgacatcg gctatgcgat ggtctgctat cccgactatg
     3961 ccgtggcgaa ggggctcaac ccaaaattgc agctcatccg caccaaaact ttgatgcagg
     4021 gcgacgacag ttgcagccta cgatacgtga tgcaagcctg accgatccgt gccgagctac
     4081 gccagaggac cggcgttatg gtcagccacc cggaagttca aactggaacg gatcttacgc
     4141 tactaattat gtccattggc cgcaagacct gaccgacgcc agtgggcctt gcttgaccag
     4201 actttgggga aacgatactc ggaccgcaac cgtcaacccc gaaaactgtc cacgtgctgt
     4261 aaccccggcc cggccggggc cgctgttcct ggctggcgcc gcggcagtgg ggttcaggga
     4321 acttgccagg ccgtctccgt gcaacgcatt gtgtatactc ggcttccagc cgcacgctac
     4381 ttatcaggcg ggggcgcggc caccgtcatg ccatggcgtc ggaagacatc cgtcatcttc
     4441 gcgcggtcag gcgggccgcc ggcggcggca ttgattactt cggcagcttc acggaagtac
     4501 gcggggccca ttatcgcagg ggttatgacg gcgagctgct tcacatcctc gctgccgagg
     4561 ttatcgaaac ggtgcaccgc gcctcggggg atgcacagcg cctgcccggg gccaacctca
     4621 atgggcgtgc cgtcaaccgt ccaggtaagg acgccctcga taccgtagag aatctcctcg
     4681 taggcgtcat tcttgtgagc cggcgccgcc agcttctgcc cgacggggac tacaacctcg
     4741 aacacggaca cgctgccgtt ggaatcatca cccgtgagca ggaaccggat ccctagcgga
     4801 ccaatcttga tggtctcttc tgaaggattg actctggtcg tactctgctg cataatcgcc
     4861 gccttttaaa ctaagttatc tgacatctcc ggtttggcca acccggaacg attgagtcct
     4921 ctgtcggaga agcgtggaac ccttctgatg agcagcccct tggagccaca cggaagagca
     4981 taacagatga gcaagtcgcg cccgctcctg gcgtccccac cgcaaagcga gcggtagccc
     5041 cgatctactt tcgccccccg ttgcggtcga gtccgaactt cgggtgctgc gacgttccgt
     5101 ccaccttaat gttcaagaac gtccccgcgc cgcgcttcgc aaagaatgga tcaaccggct
     5161 tcagagccca tcgtttccat ccggtcaccg tgcccgagac cttggactgc agccgcaggt
     5221 tgccgtgtag atcaagggcg tcgtttgcca gattgtaacc gcctgcaagt tgcacatggg
     5281 cgcccggcac accgaacgcg agcgagcgga atgacagcgc ttcattctcc aaccgaaact
     5341 cgccggtcat ataggaagcc acttcgtcga tgtcggtgtt cgtcggagcg ccctggccgc
     5401 ggcggctgag gttgtcgatc tgatcctgga tggtcgtgcg caggaacttg ccttccagga
     5461 gttcaaaccg cccatcgagg atgagcttct cgcgcacctt tcccgacagt ggcgggatat
     5521 cgatcctggt atcaagcatc acgcggcctt ccatgaacgg cggacccttg gcggccaggc
     5581 gaagcaggtc gcgaatggcg ccgtcgggca tcgaaacgtt cagtgaaacg gtgcggcgct
     5641 gttcgccttc atgcctgatc accgcgccgc tggtgagaaa gctcgtggaa cccaaagtgg
     5701 cgtggacagg cctcaaaacc gtgttcccat tcgtgccatc caccagcacc tcgaacttcg
     5761 tggtaagggg caccggatta ttggccatct ttagccgaaa atcagggacc ttggcttcgc
     5821 cccgcgcctc gatcgcggcg agcgtgcctt ggaagttgcc ggtggaagtg aggatgccgg
     5881 cgatcccggc gaacacgccc aggtcggcct tctcgaaaac gtactcgccg gccagcggcg
     5941 aatcgcctgg ttcgcccgcc gcccaggggc cgaaggttcc tttgctcacg atctcgccgg
     6001 gcggcttcgc attcgtcaac cgcgcctcgt agttcatggg gacgcccggg ccggcggact
     6061 cgagctccag gtggtgaatg tcgaaccgaa gcggcacctt cttcggatcc ctcggcaggg
     6121 tcaccagcac ggctttgctg atcacgactt tttcgataag cacagagggc aggcttccgt
     6181 ccggcgccgg actggcgcct ttcgattccg gagcgccccc agcgcgtttc gccggctgct
     6241 ggctgcgcgg tggcacgttg atttccatgc cgtcgagcac gaccagcggc accgtccggg
     6301 ttgcgtcgaa gagcttgccg agatcgattt cgaaagcgaa tttccggatc gcgaatatcg
     6361 gcggtagctc gggtcggctt cgcagacgca tggtcaggcc ctcgccgtcc acgtgagtaa
     6421 tgacgcctcg gccgcgcttc atcagcaggc gtagcggcga cgtccgcggg aggcgcacgt
     6481 gcaacgcgcg cagttccacc tcgctcgcga accgcttttt gaggtactcg acggcctgct
     6541 cgcgaacata cggctcgaag cggcgggaca gaatgcgcgc ggtgatgagt cctgcaacgc
     6601 ctagaatcag gaggacaaag ccagcgccga taacccagag tttgtacttg cgcttcgtag
     6661 aatcattatg gcatgcaccg tcgcgcatgg cgaacagcgt tggtgtgctt cgtacagcgg
     6721 cgcccgggaa gcttcctgta gcatgtgttg tatggaggga tctcgcttga aacccgcgct
     6781 ccttctggcg tttctgttta ttccggcggt gccacagaca caggactcaa cacctgtacg
     6841 gcgcgcccga acggttcttg agcaaggcgc cgccgatccc gatcccgacg tccgccgcga
     6901 agtcgcggtt gcgcttggcc tgccgtcacg gcgcgatcct tccaccaacc ttctcgcaaa
     6961 actcgccgcc gacaaagacc accttgttcg ggaggcggcg ctggtaagca tcggtgagct
     7021 tcgcgatcct cggctcgcca aacctgcgca ggacgcgctc gaagatgaag tgccggaggt
     7081 ggcgttcgcc gccgcacgga cgctgttcaa gctcaatcaa ccggcaggca agcaactgct
     7141 cattgagatc gtccaaagag aagcgcgtgg gaagaccggc tttgtgcgcg ccaagctgcg
     7201 cgacgcagcc aggcacatga gcaggccgaa atcggcgatg ctgttcgtgg cccaacaggg
     7261 cgtagggttt gtcccggtgc ccggactcgg agggggcttc tcggcgatgg gctcgctggt
     7321 gggcgacccg gatttctcgg cccgcgcgac ggcgctcatt gtgttgtcgt ctgaccgatc
     7381 acccgaagtt cgcagccaga ttgagcaggc gttcaacgat tcggactggt cgatgcgcgc
     7441 cgccgccaca cagatcgcgg catcgcgcaa cgaacggtcg tggcgcttcc gtctggttcc
     7501 gctgttcgag gacaccaacc ggcgcgttcg atatcgggcg gcggcgtcct ttctgcggct
     7561 cagcgggccg gcggctaggt aaagggcgcg ggcattgaag tatggatg
//
LOCUS       DATIFQ010000367         2184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_505313, whole genome shotgun sequence.
ACCESSION   DATIFQ010000367 DATIFQ010000000
VERSION     DATIFQ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505313"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VLE22_17760"
     CDS             <1..392
                     /locus_tag="VLE22_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16306.1"
                     /translation="RAGPAGDTCCHNFKLLGRAAGAQYGIIPVPYDFDVSGLVNPPYA
                     LPPDGLGIDTVKQRRYRGYCVHNRQALGVAAEFRAKRAEIVGVLGTVPGLEGGRQQKA
                     AAYLEGFFQDIATDEDVNRRLLKTCIR"
     gene            complement(385..1056)
                     /locus_tag="VLE22_17765"
     CDS             complement(385..1056)
                     /locus_tag="VLE22_17765"
                     /inference="COORDINATES: protein motif:HMM:NF027640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HSB16307.1"
                     /translation="MLALRLFAKLTAYYVAVTAIVLIALRLFPYLREYMPFGGVEALM
                     SGPRTTGLDGMLAPSPAAAEIGNLRESLYWLTITILGALLTALPGSWVYIAIRNPKDY
                     DQSLVDTIIILPIVVTSIIIIVQNSLALAFSIAGIAAAVRFRNSLKSSGDALFILLAV
                     GIGLSAGIGALELAIVMTIAFNYTFLILWVTDYGDRRGLKKRYMTDCDPDVKPAAAAS
                     PADLT"
     gene            complement(1278..1976)
                     /locus_tag="VLE22_17770"
     CDS             complement(1278..1976)
                     /locus_tag="VLE22_17770"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HSB16308.1"
                     /translation="MNRTAIFAFCLVSLASPAIAAERTLSVTTFDRVRVDGPYKVKVT
                     TGVSPYAKVSGSSAAIDGVSVDQQGQTLIIRANPSSWGGAYPGEGRGPVEISVGTLNL
                     SAAWVNGSGSLAVDRVKGLSFNLAVQGSGSAAIGNAAVDQMEIGIAGAGSVILAGAAP
                     KLTAIVRGTSSLDASALAVKDVTVGAEGPSQVRLNVSNSAKVDARGVASVEIAGGPAC
                     TVKAQGSAVVTGCR"
BASE COUNT          426 a          667 c          702 g          389 t
ORIGIN      
        1 tgcgtgcagg cccggcaggc gatacctgct gccacaattt caagctgctc gggcgagcgg
       61 cgggtgcgca atatggcatt atccccgtgc cctacgactt cgacgtctct ggactggtca
      121 atccgcccta tgcgttgccc cctgacggtc tggggatcga tacggtcaaa cagcgtcggt
      181 atcgcggcta ctgcgtccac aaccgccagg cgcttggggt cgcggctgag tttcgagcga
      241 agagggcgga gattgtcggg gtgctcggaa ccgtgccggg cctggagggg ggtcggcagc
      301 agaaggcagc ggcctatctg gaaggcttct tccaggacat tgccaccgac gaggatgtca
      361 accggcggtt gctgaagacc tgtatcaggt aagatcggcg ggagacgctg ccgcggccgg
      421 tttcacgtcc ggatcgcagt cggtcatgta gcgtttcttg aggccgcgcc ggtctccgta
      481 atcggtcacc cacaggatca ggaacgtgta gttgaacgcg atcgtcatca cgatcgccag
      541 ctccagcgcg ccgatcccgg ccgacaggcc gatgccgaca gcgagcagga tgaacagggc
      601 atcgcccgag ctcttgagcg aattgcggaa gcgcacggct gccgcgatcc cggcgatcga
      661 aaaggcgagc gccagcgagt tctggacgat gatgatgatg ctggtgacga cgatcggtag
      721 gatgatgatc gtgtcgacca gcgactggtc gtaatccttg ggattgcgga tcgcgatata
      781 gacccaactg ccgggcaggg ccgtcagcag cgcgcccagt atggtgatcg tcagccagta
      841 gaggctctcg cggagattgc cgatctcggc cgccgccggg ctcggcgcga gcattccatc
      901 gaggccagtg gtccgcgggc cggacatcaa ggcctcgacc ccgccaaatg gcatatattc
      961 gcgaagatac ggaaatagcc gcaacgcgat caggacgatc gcagtgaccg cgacataata
     1021 tgcggtcagc ttcgcgaaga gacgcagcgc cagcattacg agcatcctga cacaagcgaa
     1081 acgaaagaag aagcgcagcc gcctatcgac ggctgcgcct tctattctgg tctaatctcg
     1141 aactgttctc agaacaggat gatcaaaagg atgatcaaca aaatgagacc gccgataccg
     1201 atatacatcg cttggctccc actgttttgg tgtggtgagg taacgattcc gggtcgagcg
     1261 aagttccggt gactaggtta gcggcaaccg gtgacgacgg cagagccctg cgccttgacc
     1321 gtgcaggccg gaccgcccgc gatctccacg ctcgccacgc cgcgggcgtc gaccttggcg
     1381 ctgttgctca cgttgagccg cacctgtgag gggccctcgg ctccgacggt gacgtccttt
     1441 actgcaagcg cggatgcatc gagcgaagag gtgccgcgaa cgatcgcggt tagcttcggc
     1501 gcagcaccgg ctaaaatcac gcttccggcg ccggcaatgc cgatctccat ctggtcgacg
     1561 gcggcgttgc cgatagcggc cgagccggac ccctggacgg cgaggttgaa ggatagtccc
     1621 ttcacccgat ccacggccag cgacccggag ccgttgaccc aggcggccga cagattgagg
     1681 gttcccacgg agatttcgac cgggccgcgt ccctcgcctg gatacgctcc gccccatgac
     1741 gaggggtttg cccggatgat cagggtctga ccctgctggt cgaccgagac tccgtcgatg
     1801 gcggcggatg atccgctgac cttcgcgtaa ggcgagacac ccgtcgtgac cttcacctta
     1861 tagggtccat cgacgcgaac gcgatcgaag gtcgtcacgc tcaaggtgcg ctcggccgcg
     1921 attgccggcg aagcaagtga gacgaggcag aaagcgaaaa tggcggtcct gttcatgccg
     1981 ccactctcgc ttgtcatggt taagatgtca ctatgaacag cgggccgagc cggagcctgc
     2041 cttcgagacc gtgcacttgg cgccgccggt catctcgacg ttgcccgatc ccatgatgtc
     2101 gaccgaggcg gtcgtgcttg cctgacccgc gacatctccc gatcccgcaa tggagaccgc
     2161 cgcggtttcg acagcgacgc cctt
//
LOCUS       DATIFQ010000368        15092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_506137, whole genome shotgun sequence.
ACCESSION   DATIFQ010000368 DATIFQ010000000
VERSION     DATIFQ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15092
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506137"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..2259)
                     /gene="fusA"
                     /locus_tag="VLE22_17775"
     CDS             complement(166..2259)
                     /gene="fusA"
                     /locus_tag="VLE22_17775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSB16309.1"
                     /translation="MPRAVSLEKVRNIGIMAHIDAGKTTTTERILYYTGRTYKIGEVH
                     DGTAVMDWMEQEQERGITITSAATTCEWRDVQINIIDTPGHVDFTAEVERSLRVLDGA
                     VAVFDAVAGVQPQSETVWRQADKYRVPRICFINKMDRVGADFFQSVESIVNRLKCKPV
                     PIQIPIGSEDQFRGVVDLIEMRARVWRDETLGAEYDDVEIPAELREQAESYREKLMEA
                     AAESDDELLTKYIENHSLTNEEIQRGIRAGAISLRIFPVICGSAFKNKGIQRLLDAVV
                     DYLPSPVEVPPVQGVAVDDPDAVVERKSSDDEPFSALVFKIMTDPFVGQLAFLRVYSG
                     KLASGDTVYNVAKGRKERMGRLVRMHANKREEVHEVYAGDIAAAVGLKGVTTGDTICD
                     EKAPVILESIEFPAPVIQLAIEPKTKADQEKLGMAIGKLIQEDPTLKISTDTETGQTI
                     LAGMGELHLEIIVDRMQREFGVGANVGKPQVAYRETIRGTSEAEGRFIRQTGGRGQYG
                     HVKLRLEPLPSGAGFEFVNGIVGGAVPREYVPAVEKGVAEALEGGVLAGYPMEGVKVT
                     LYDGSYHDVDSSEMAFKIAASMGFKQAAEKAKPVLLEPVMSVEVIVPEEYMGDVIGDL
                     NSRRGRIEGMELRGTSQIIKSMVPLAEMFGYATDLRSRTQGRGSFTMHFGRYEEVPTA
                     ISGEIISRVQGRVSR"
     gene            complement(2269..2739)
                     /gene="rpsG"
                     /locus_tag="VLE22_17780"
     CDS             complement(2269..2739)
                     /gene="rpsG"
                     /locus_tag="VLE22_17780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSB16310.1"
                     /translation="MPRRRRIEPRENAPDPVYNSTLAQRFVNSMMWDGKKGTAQGIFY
                     TAMNKIQERSGDDPLKLFKKAVENCKPLLEVKTRRVGGANYQVPVEVPQNRRISLAIR
                     WLILNARSRPEKGMPEKLANELTDAANLRGGAIKKKDDVHRMAEANKAFAHYRW"
     gene            complement(2746..3120)
                     /gene="rpsL"
                     /locus_tag="VLE22_17785"
     CDS             complement(2746..3120)
                     /gene="rpsL"
                     /locus_tag="VLE22_17785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSB16311.1"
                     /translation="MPTFNQLVRKGRRPPRYKTASPALQACPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNTIEVTTYIPGVGHNLQEHSIVLIRGGRVKDLPGVRYHVIRGTL
                     DTAGVADRKQGRSKYGAKRPKS"
     gene            3484..6009
                     /locus_tag="VLE22_17790"
     CDS             3484..6009
                     /locus_tag="VLE22_17790"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HSB16312.1"
                     /translation="MREPLVLPAAAVTVGIAASRYVGFGGRGLLVQISALAGLWLLAR
                     RGSSRVLPGTCLLLALFFGGAWLELFHRPGPPPEIDVGPREIVILSGCLVEPPVRFED
                     RLQFTLELSPSAAARVSLYLREGETPPTLRYGQRIEIEARVRRPRNFGNPGSFDYSGY
                     LARRNVFWTAYARPGATLTVLRGDCGSAFRRFVFDLRTSALGRIESLYSRSPYESAMM
                     QAVLIGEKAKLEKAWTEHFRSTGTYHALVISGLHVTVLAAVVLFFLRVCALPPGAVLI
                     TASACAWLYALVAGWQAPVIRAAAGLTLFLAARWFFRKPRLLNLIAATGLGFLIFDPA
                     QLLEASFQLSFLSVAAIASLAVPLLETTSVPFARGAAGIVDRNRDIHLPPRVAQFRVE
                     LRLLAETIALWTRLPERFTLPLFAAMCRAALYVYELAVVSAAVQVGLALPMALYFHRF
                     SISGLSANLVVVPLTSILVPVGFLAIFTGWSLPARLAAWLLDATRLVVDAHARWEPWW
                     RIPDPPLWLAAAFSASLILFGLAVRNRPVWRWLTGGVVAGLLFLILLHPFPPATTPGQ
                     LEFSAIDVGQGDSSFLSLPAGPLVLVDAGGTSGFGRRNGGLDPGEEVVSPYLWSRSIR
                     RLNAVVVSHAHEDHIGGMEAVVRNFRPRELWAGALPEVPAWRQLRDVAHRQGVAIRRL
                     SAGQRFPYGGALVEVMAPLEGRTPAEAPSDLDSLVLRISFGNCSVLLTGDLERCVESV
                     LLESDRLKSAGILKVAHHGSRRTTSEEFLRAVRPVFAVISAGADNSYGHPHPEVLRRI
                     EEARATALRTDVFGLVSLRSDGRRFALTTWNGMERESGLLRPF"
     gene            complement(5992..6489)
                     /locus_tag="VLE22_17795"
     CDS             complement(5992..6489)
                     /locus_tag="VLE22_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16313.1"
                     /translation="MSDSDWVTVFRSGEHSAEEEASAACELLRDQGIRAELADADSPG
                     VPFGTWEVRVPATEGARAESIIAAQGAEAAPHGDASHDMDLVTLFTSDAHNGEMEAMA
                     IQGILEAGGIPSVLVGFQAMPSLPFEVRVPSARADEARRLIAESQAGGPEAAERAEKA
                     SERPE"
     gene            6619..6882
                     /locus_tag="VLE22_17800"
     CDS             6619..6882
                     /locus_tag="VLE22_17800"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HSB16314.1"
                     /translation="MAEDNNSRAVWFLMGAAVGGAIALLFAPKSGRDTRRYIVKKTEE
                     SRDALADAGKELLDRGREYYEKGRKIAEEAGEVFERGRKLVKG"
     gene            6961..8502
                     /gene="pruA"
                     /locus_tag="VLE22_17805"
     CDS             6961..8502
                     /gene="pruA"
                     /locus_tag="VLE22_17805"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HSB16315.1"
                     /translation="MFHNEPYTDFRDSSNRQVMEQALRNVRARFGREYELLLAGARLK
                     TEEKLHSRNPSRPAEIVGTHQKATPALAGMAVETAFSFFPEWSRTPAESRAAMLLRAA
                     AIIRRRKMEFDAWLVYEAGKTWPEAEADVSEAIDFCEYYARGMLQLAPPKPMLHLPGE
                     RDELHYLPLGVGVIVSPWNFPLAILAGMTTAALVTGNTVVIKPSSDTPTIAAQFAEVL
                     LEAGFPEKCFCLLTGSGASVGDVLVQHPRTRFVCFTGSRDVGLHINELASRPQPGQVW
                     IKRVIAEMGGKDAIIVDDEADLDSAVDGVLVSAFGFQGQKCSACSRAIVHQKIYDEFL
                     DRLKTKVETIKVGSADDPDNYMGPVINEGALRSIRAYIDTGRKEGRLITGCPPVSGDG
                     YFLRPTVIADVEPRARIFQEEIFGPVLAVTPARDFDHALELANDTEYGLTGGVYTHNP
                     EKIRKARERFFVGNLYVNRKCTGAMVGAHPFGGFNMSGTDSKAGGPDYLMQFLQAKSI
                     AEKIA"
     gene            complement(8509..9531)
                     /locus_tag="VLE22_17810"
     CDS             complement(8509..9531)
                     /locus_tag="VLE22_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16316.1"
                     /translation="MKTAPFLPVLVLALAGAAFSQRKTGSPLDHLPANIEILTHFGER
                     ADISPDNQRVAFMAKSFGDAFVIDLKARAIHCLTCGIPGAAFLRIMHLANGDYILIGP
                     ERFKDIRTSRGPENELWFLSRKPGSKPVRFGQRMSEGAAVSKKHMTIAYAETSSQNPE
                     LPKGASRLVTAEVKLEGGSPRLVEKKVVHESASMGCTIEAQDFYDDDSKLTFSCYEPG
                     DTASVMSVDLKTGKVTNMSQASGTYNEPEGIFPDGQYTLVEADRQVQTIGGKGGFRQI
                     DIWKLRLDGTGKDFTRLTHFNDYEGWKASNPVVSTDGRFMAFQTAKTADEAGVGYGIL
                     LYRFGR"
     gene            complement(9543..10508)
                     /gene="murB"
                     /locus_tag="VLE22_17815"
     CDS             complement(9543..10508)
                     /gene="murB"
                     /locus_tag="VLE22_17815"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HSB16317.1"
                     /translation="MPSTETQKRLAAIPNLQVLHDVPLARYTRFGIGGPADLFAETAS
                     VEAFVAATEAARDSVIPFLLLGGGTNLIVSDEGYRGLVLRFSAKELRAEGSTVFAEAG
                     AELEALVDFTIERGLKGMETLKRIPGSVGAAVYGNAGAYGHSISERVRSVRFFDGHGV
                     RIFDNAGCEFAYRESVFKRRKDWVIFSTELLLEPADPEELKRTASEIQAIRDEKFPVT
                     MKCAGSIFKNLLLAALPSDVAAQVAPGVVREGKVAAAYFLEQVGAKGMARGDMRVADY
                     HANLIYNAGGGTARELRELIAELKSRVRARFGLELEEEVQYAGFE"
     gene            10536..11252
                     /locus_tag="VLE22_17820"
     CDS             10536..11252
                     /locus_tag="VLE22_17820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HSB16318.1"
                     /translation="MRTEFPGFPPEAMSFFRGLKRNNNREWFQARKPIFDEKVRAPME
                     RLVEAVNAALIRFAPDYVTEPGKAIYRIYRDTRFSRNKVPYKTQIAAVFTRRGLSKNG
                     SAGLYFSVSPEEIEVAGGVYMPDREQLLAIRNCLAGQYAEFRRILARKGLRTLMGGLW
                     GEQLSRVPKGFPAGHPAEDLLRYKQWLVYVLLDPGIATTPDLTGEIVTRFRAMVPFVE
                     FLNKPLSRKRSEVSVENFFA"
     gene            complement(11472..12653)
                     /locus_tag="VLE22_17825"
     CDS             complement(11472..12653)
                     /locus_tag="VLE22_17825"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HSB16319.1"
                     /translation="METVLQHPLFKLLIVFVAVWAVFPLVAGYLSLMERKVLADLQVR
                     LGPMRVGPHGLLQPIADALKLILKEDLIPRGADQKIFWLAPVISTFTALTAFSVIPFS
                     SFIYAADVNVGLLVISAMSALGIVGIILGGWASNSHYPLLGSLRSAAQLVSYEVVLSL
                     ALVSVIMMAGTLSMVGIVKAQMARHVWFAFDNFGLMIVPFAMYVIGGVAETNRSPFDL
                     PEAESELVGGFHTEFSGFRFALYFLAEYANLLVVASVAVTLFLGGWLRPFPNVAWLEY
                     PLNYGLPALIFAGSGIATFPMVRRLKSEVQKVTLVAVALFLLLAAALFLIPAVNGAVI
                     GMFWFLLKAGTMIYMMIWVRGTFPRFRYDQLMNVGWKYLIPIGMGAILVNAAVGMLKA
                     G"
     gene            complement(12661..14130)
                     /locus_tag="VLE22_17830"
     CDS             complement(12661..14130)
                     /locus_tag="VLE22_17830"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSB16320.1"
                     /translation="MSQFYTSTDHFVLVPAIMLALFGCAVLLFDFFLFPDPKQRKWLL
                     IFVVLGIVFAGTGLWRQQSYLASQGINELTAFGGSLTVDGFALFFNWVFLAASLIVAL
                     VSYRYLEIEGEHHGEYYGLILLANCGMFFLATGTDMITLFVGLELMALCFYVMVGFLR
                     GERRSNEAAMKYLLLGAFSTGFLVYGFSVFYGLSGSTKLSAIADAVASREPWDPLVLL
                     ALVTTSVGLLFKISAVPFHMWAPDAYEGAPTTVTAYLSVASKAASFAFLVRMFFGPLA
                     SVRELWEPLLAVVAIASMTVGNIAAMTQSNIKRMLAYSSISHAGYILLGVIAGNDTGI
                     KGVLIYMLVYTFMNLGAFLVVVALRRKDLLGEDVDDLAGLMHRSPGYAVLMLIFMLSL
                     AGIPPTAGFIGKYWIFLSLIETGHYVLAVVATLYMAVAIYYYFRVVRSMFIAEPADAA
                     PLSTSFGLRLALGVTGLMTLGIGIYPQPFIMMAQTSLLR"
     gene            complement(14127..>15092)
                     /locus_tag="VLE22_17835"
     CDS             complement(14127..>15092)
                     /locus_tag="VLE22_17835"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSB16321.1"
                     /translation="FGPSLSFWLFLALFVAFAIKLPVFPFHTWLPDAHVEAPTAGSVI
                     LASVLLKMGTYGFIRFSLPLLPKASIEPWIVQVLIGLSIVGIIYGALVSLMQRDWKKL
                     VAYSSVSHLGFCTLGIFALNPNGIAGSVIQQVNHGISTGMLFLIVGVIYERRHTREIK
                     EYGGLAHVMPIYSIVFAFAMLSSAGLPLLNGFVGEFTILQGAFEENRAWAAFAVTGVI
                     LGAAYLLWLYQRTMLGQVTNAKNLKLPDLSLREVAVFLPLIAWSIWIGVYPKPYFEIL
                     EKPVAQLVERVRPGYFQGGAQAKTDLGRPAPRPGAWMPVQQEAAR"
BASE COUNT         2789 a         4986 c         4427 g         2890 t
ORIGIN      
        1 ctccggaact tcaccttcgg gttgttcttc gccagacact tcgtgatcgc cgccgtcagc
       61 gtcgtcttcc cgtgatcgat gtgcccgatc gtcccgatat ttacgtgcgg cttgctgcga
      121 tcgaattttt ctttcgccat atcgtttagg attctcctcg ctgagctatc tgcttactct
      181 tccctgcacc cggctgatga tctccccgga gatcgccgtg ggtacttcct catagcgccc
      241 gaagtgcatc gtgaagcttc ctcggccttg cgtccgcgag cgcaaatccg tcgcatagcc
      301 gaacatctcc gccagcggca ccatcgactt gatgatctgc gaagtgccac gcagttccat
      361 gccctcgatc cgcccgcggc gggagttgag gtcgccgatc acgtcgccca tgtactcttc
      421 agggacgatc acctcgacgc tcatcaccgg ttcgagaaga accggcttcg ccttctccgc
      481 ggcctgcttg aagcccatgg aggcggcgat tttaaacgcc atttccgacg agtccacgtc
      541 gtggtagctg ccgtcgtaga gcgtgacttt cacgccttcc atcgggtagc cggcgaggac
      601 gcctccctcg agcgcctcgg ccacaccctt ctcaacggcc ggaacgtatt ccctcggtac
      661 cgctccgccc acaataccgt tgacgaactc gaatccggcg ccggaaggca gcggctcgag
      721 ccgcagtttg acgtggccgt actggcctcg cccgccggtc tgccggatga atcgtccctc
      781 ggcttccgag gtgccgcgaa tcgtctcgcg ataggccacc tgcggctttc cgacgttcgc
      841 gccgacgccg aattcccgct gcatccggtc gacgatgatc tccaggtgca actcacccat
      901 tccggcaaga atggtctggc cggtttcggt gtcggtcgag atcttcagcg tggggtcttc
      961 ctggatcagc ttgccgatgg ccatccctag cttttcctgg tcggccttcg tcttcggttc
     1021 gatggcaagc tggatgacag gcgccgggaa ctcaatcgac tcgaggatca ccggggcctt
     1081 ctcgtcgcag attgtgtcgc ccgtcgtgac gcctttcagc ccaaccgccg ccgcgatatc
     1141 gccggcgtaa acctcatgga cctcttcgcg cttgttggcg tgcatcctga caagcctgcc
     1201 catccgctcc ttccggccct tggcgacgtt gtaaacagtg tcgccgctcg ccagtttgcc
     1261 tgagtaaact ctgagaaacg caagctggcc gacaaacgga tcggtcatga tcttgaagac
     1321 cagcgccgaa aacggctcat cgtccgagga cttgcgctcc acgaccgcgt cggggtcgtc
     1381 cacggccacg ccctgcacgg gcgggacctc gaccggagac ggcaggtagt ccacgaccgc
     1441 atccagcagc ctctggatgc ctttattctt gaaagccgaa ccgcagatga ccgggaagat
     1501 tcttagggaa atggcgccgg ctcggatgcc gcgctggatc tcttcgttgg tcagagagtg
     1561 attctcgatg tatttcgtca gaagttcatc gtcactctca gcggcagcct ccatcagctt
     1621 ctcgcgatag ctctcggcct gctcgcgcaa ctccgccggg atctccacgt cgtcgtattc
     1681 ggcgcccagc gtctcatccc gccagactcg ggccctcatc tcgatcaggt cgacgacgcc
     1741 gcggaactga tcctcgctgc cgatcgggat ctggatcgga accggcttgc acttcaggcg
     1801 gttgacaatg ctctccaccg actggaagaa gtcggcgccc actcgatcca tcttgttgat
     1861 aaagcagatc cgggggaccc gatacttgtc ggcctggcgc cagaccgtct ccgactgcgg
     1921 ctgcacaccc gcaactgcgt cgaaaaccgc cacagcgccg tccagcacgc gcaggcttcg
     1981 ttccacttcg gccgtaaaat cgacgtggcc cggcgtgtcg atgatgttga tctgaacgtc
     2041 tcgccactcg caggtcgtgg ccgccgaagt gatggtgatt ccacgttcct gttcctgctc
     2101 catccaatcc atgacggcgg taccgtcgtg cacctcgccg atcttgtaag tgcggccggt
     2161 gtaataaagg atccgctcgg tcgtagtggt cttgccggca tcgatgtgag ccatgatgcc
     2221 gatgttccgg accttctcga gggataccgc cctaggcatg aagcaccatt accaccggta
     2281 gtgggcgaac gctttgttcg cctctgccat gcggtggacg tcgtctttct tcttaatcgc
     2341 tccgccgcgg aggttcgcgg cgtccgtcaa ctcgttggcc agtttctccg gcatgccctt
     2401 ctccgggcgc gaacgcgcgt tgagaatgag ccatcggatc gccaggctga tccggcggtt
     2461 ttgaggaacc tccaccggca cctggtagtt ggcgccgccg acgcggcgcg tcttcacttc
     2521 gaggagcggc ttgcagtttt ccaccgcttt tttgaagagc ttcaatggat cgtcacccga
     2581 ccgctcctga atcttgttca tggcggtgta gaagatccct tgtgcggtgc ccttcttgcc
     2641 gtcccacatc atcgagttga cgaatctctg cgccagcgtc gagttgtaaa cgggatccgg
     2701 cgcgttttcg cggggttcaa ttcttcgtct gcgtggcatg gctgcttacg atttcggcct
     2761 cttggctccg tactttgaac ggccctgttt ccggtcggcg acgcccgcgg tgtcgagcgt
     2821 cccgcgaatc acgtgatatc gaacgcccgg aaggtccttc acgcgcccgc cgcggatcag
     2881 cacgatcgag tgctcctgca ggttgtgacc gacacccgga atgtacgtcg tcacctcgat
     2941 agtgttcgtc agccggacgc gcgccacctt gcgaagcgcc gagttcggct tcttcggagt
     3001 tgtggtgtag acgcgggtgc aaacgccgcg ccgctgcgga catgcctgca atgcaggact
     3061 ggccgtctta tagcggggtg gtctgcggcc ctttcgcacg agttgattaa acgtcggcac
     3121 ggatcttcct cagttccttt gcttgagtct ctttcctcaa aactcggtcg gcacaggacg
     3181 gctcacatca agcattcctg cgcatcccaa cgttgcgact cgcccaaaca atgagcggac
     3241 cggattagca ctcaacaatt gcgagggtac tggcttcgga ctagcccttg cttcaccggc
     3301 ttcgatccgg tggaacagcg gggccgaccc ccggccggcc tatccaatta gacaggggta
     3361 gcacaggggc agaagagtta actcgccaaa ccctactagc ataacgaaaa gcccgaaacc
     3421 tgtcaacaac gggagaggcg tcagtgcctt cagccactat ctcattggag atccgccact
     3481 tccgtgcggg agccacttgt ccttccagca gccgccgtga ccgtgggaat cgccgcgtcg
     3541 cggtatgtgg gcttcggcgg ccgcgggctg ctcgtgcaga tttcggcgct ggcgggcctc
     3601 tggctgctgg cacgccgcgg gtcaagccgc gtgcttcctg ggacctgcct tctgctggcc
     3661 ctcttctttg gtggcgcctg gttggaactt ttccaccgcc ccggtccgcc gccggagatc
     3721 gacgttggcc cccgcgaaat cgtgatcctc agcgggtgcc tcgtagaacc gcccgtccgg
     3781 ttcgaagacc gtcttcagtt cacccttgag ctgtcgccct cggctgccgc ccgcgtcagc
     3841 ctttacctgc gggagggaga gacgccgccc accctgcgtt acggccagcg gatcgaaatc
     3901 gaggcgaggg tgcgccgtcc caggaacttc ggcaaccccg gctctttcga ttattccggc
     3961 tatctcgccc ggcgcaacgt cttttggact gcctacgcgc ggcccggcgc gacactcacc
     4021 gtcctccgcg gcgactgcgg ctcggccttc cgccggttcg tcttcgacct gcgcacctcg
     4081 gccctcgggc ggatcgaatc gctttactct cgaagcccat acgaatcggc catgatgcag
     4141 gccgtgctca tcggcgaaaa ggcaaagctc gagaaggcct ggaccgaaca cttccgttcc
     4201 accggcactt accacgcgct ggtgatttcc ggactccatg tgaccgtgct tgccgccgtg
     4261 gttctcttct ttctgcgtgt ctgcgccctg cctccgggcg ccgtactgat caccgcctcg
     4321 gcttgcgcat ggctctacgc gctggtggcc ggatggcagg ccccggtaat ccgggcggcc
     4381 gccgggctga cgctgttcct ggccgcgcgg tggttcttcc gaaagcctcg cctcctcaac
     4441 ctcattgcag ccacgggact cggctttttg atcttcgacc cggctcagct tctcgaggcg
     4501 agtttccagt tgtcgtttct gtcggtggcg gcgatcgcct cgttggcggt tcctcttctc
     4561 gaaacaacct ccgtgccgtt tgcccggggc gcagccggaa tcgtggatcg caatcgggat
     4621 atccatctcc cgccccgcgt cgcccagttc cgggtcgaac tccgcctgct cgccgagacg
     4681 atcgccctct ggacccggct gccggagcga ttcaccttgc cgcttttcgc ggcgatgtgc
     4741 cgggccgctt tgtatgtcta tgaactcgct gtagtctcag cggccgtcca ggtcggcctg
     4801 gctctcccga tggcgttgta tttccaccgt ttctcgatct ccgggctgtc ggccaacctt
     4861 gtcgtcgtgc cactgacgtc gatcctggtt ccggtgggct ttctggccat cttcacgggt
     4921 tggagtctgc ccgcccggct ggcggcgtgg cttctcgacg cgaccaggct ggtggtcgac
     4981 gcacacgccc gctgggaacc gtggtggcgt atcccggacc cgcccctttg gctcgcggcc
     5041 gcattctccg cttcactcat cctctttgga cttgcggtgc ggaaccgccc ggtctggcgg
     5101 tggctgactg gcggtgtcgt cgccgggctg ctctttctca ttctcctcca cccgttcccg
     5161 cccgcaacca cgcccgggca attggagttc agcgcgattg atgtcggcca gggagatagt
     5221 tccttcctct ctttgcccgc cggaccgttg gttctggtgg acgccggcgg aacctcgggg
     5281 ttcggtcgaa gaaacggcgg cctcgatccg ggcgaggagg tcgtctcgcc ctatctctgg
     5341 agccgatcga tccgccggct caatgccgtc gtcgtctcgc acgctcacga ggaccacatc
     5401 ggcggcatgg aagccgtggt ccggaatttc cggccccgtg agctgtgggc cggcgccctc
     5461 ccggaagtcc ccgcctggcg gcaactgcgg gatgtcgcgc atcgccaggg cgtcgccatc
     5521 cgccggttgt ccgccggcca gcgtttcccg tacggcggcg ccctcgtcga agtcatggcg
     5581 ccactcgaag gtcggacgcc ggccgaagcg ccgagcgatc tggattcgct cgtcctccgg
     5641 atctcgttcg gcaactgctc cgttctgctg accggcgacc tagagcgttg tgtggagtcc
     5701 gtgctcctcg aatcggaccg gctgaaatcg gccggaatcc tgaaggtggc gcatcacgga
     5761 agccggagga cgaccagcga ggaattcctg cgcgctgtcc ggccggtgtt cgcggtgatc
     5821 tcggccggcg cggacaattc ctacgggcat ccgcatccgg aggttctgcg ccggatcgaa
     5881 gaggctcgcg ccacggcgct ccggaccgat gtgttcggac tggtgtcact caggtccgac
     5941 ggccggcgtt tcgcactgac aacctggaac gggatggaga gggagagcgg cttactccgg
     6001 ccgttctgat gccttttcgg cccgctcggc cgcttccggg ccccctgcct gtgattccgc
     6061 gatgagccgc cgggcttcgt cggcgcgtgc actgggcacc cgtacctcga acggaaggct
     6121 cggcattgcc tggaacccaa ccaacaccga ggggatgcca ccagcctcga ggatcccctg
     6181 gatcgccatt gcctccatct cgccgttatg ggcatcggaa gtgaacagag tgaccaggtc
     6241 catgtcatga gaggcgtcgc catgcggcgc cgcttccgca ccctgtgcgg cgatgatcga
     6301 ttccgcgcgg gcaccttccg tcgctggaac ccggacctcc caggtcccaa acgggacccc
     6361 cggactgtca gcatcagcga gttccgcccg gattccttga tcccggagaa gctcacaagc
     6421 cgccgacgcc tcttcctcag ccgagtgctc gcccgaccga aataccgtaa cccaatccga
     6481 atcgctcaca ggctgcacca tgccttgagc ataacgcagt agcggccacg tgacagattc
     6541 cttccgccgc tcgaatccgg accttccgct cggcgcgtgg gtgcgttaga tacaatagga
     6601 gcgaagggag tccgagccgt ggcagaagac aacaacagca gggcagtgtg gtttctgatg
     6661 ggcgccgccg tgggcggtgc cattgcgctc cttttcgcgc cgaaatcggg acgtgacacg
     6721 cgaaggtaca tcgtcaagaa gaccgaggaa agccgcgatg ctctcgcgga tgccggcaag
     6781 gaactcctcg atcgcggccg tgagtactac gagaagggcc gcaagatcgc ggaagaggcg
     6841 ggcgaggtct tcgagcgcgg ccgcaaactg gtgaaaggct gaccgcggcc ggcctcgggt
     6901 tatctcgttt ccgtcacagc catgagtagt ccagagtccg caaccctgtc cggactgtcg
     6961 gtgttccaca atgaaccgta cacggatttc cgggactctt cgaatcgcca ggtgatggag
     7021 caggcgctcc ggaacgtacg cgcccgcttt ggccgcgagt acgaattact gctcgccggc
     7081 gcccgcctga agaccgaaga aaagcttcac tcccggaatc cctcccgccc cgcggaaata
     7141 gtcggtactc atcagaaagc gactccggcc ctggctggca tggccgtcga gaccgcgttt
     7201 tcgttctttc cggagtggag ccgcacgccg gccgaatcca gagcggcgat gctgctgcgc
     7261 gcggccgcga tcatccgccg ccgcaagatg gagttcgatg cctggcttgt ctatgaggcg
     7321 ggcaaaacct ggcccgaagc cgaggcggat gtctccgagg caatcgactt ctgtgagtac
     7381 tatgcacgcg gaatgctgca actcgcgccc ccgaaaccga tgctgcacct ccccggggag
     7441 cgggacgagt tgcactacct tccgctcggc gtcggggtga tcgtctcgcc ctggaatttc
     7501 ccgcttgcca tcctcgccgg aatgaccacc gccgctctgg tgaccgggaa caccgtcgtc
     7561 atcaagccct cgagcgacac gcccaccatc gccgcacagt tcgccgaggt tctgctcgaa
     7621 gccggctttc cggagaagtg cttctgcctg ctgacgggca gcggcgcgag cgtcggcgac
     7681 gtcctggtgc agcacccgag aacccgtttc gtctgtttca ccggctcccg ggacgtgggc
     7741 ctgcacatca acgagctggc ttcccggccg cagccggggc aggtctggat caagcgtgtg
     7801 atcgccgaga tgggcggcaa ggacgccatc atcgtcgacg acgaggcgga cctcgacagc
     7861 gcggtcgatg gcgttctcgt ctccgcattc ggctttcagg ggcagaagtg ctcggcctgt
     7921 tcccgtgcga ttgtgcacca gaagatttac gacgagtttc tcgatcggct caagacgaaa
     7981 gtcgaaacga tcaaagtcgg atccgccgac gaccccgaca actatatggg accggtcatc
     8041 aacgagggcg cacttcgctc catccgcgcc tacatcgaca ccggccgcaa agagggccgc
     8101 ctgatcacgg gctgcccgcc ggtctccggc gacggctact ttctccgccc gaccgtcatc
     8161 gcggacgtgg agccaagggc caggatcttt caggaagaga ttttcggtcc ggtactggcg
     8221 gtgacccccg cgcgggactt cgaccacgca ctcgaactcg ccaacgacac cgagtacggg
     8281 cttaccggcg gcgtctacac gcacaatccg gagaagatcc gcaaagcccg ggagcgtttc
     8341 tttgtcggga acctgtacgt caaccggaag tgcaccggcg cgatggtggg cgcccacccc
     8401 ttcggtggat tcaatatgtc gggcaccgat tccaaggctg gaggacccga ctacctgatg
     8461 cagttcctcc aggcgaaatc gatcgccgag aagatcgcgt aagggtgtct accgcccgaa
     8521 ccggtagagc agaattccgt accccacgcc cgcttcatcg gccgttttcg cggtttgaaa
     8581 cgccatgaac cggccgtccg tcgacacgac tggattggac gccttccagc cctcgtaatc
     8641 gttgaagtgg gtcagccgtg tgaaatcctt ccctgttccg tccagccgca gcttccagat
     8701 gtcgatctgc cggaagccgc ccttgccgcc gatcgtctgg acctgccggt cggcttccac
     8761 gagcgtgtac tggccatccg gaaaaatgcc ctcaggttcg ttgtaagttc cagacgcctg
     8821 ggacatgttc gtcacctttc ccgtcttgag gtcaaccgac atcaccgacg ccgtgtcgcc
     8881 gggctcataa caactgaagg tcagctttga gtcgtcgtcg tagaaatcct gggcttcgat
     8941 cgtgcacccc atactcgcgc tttcgtgcac caccttcttc tccaccaggc gcgggctccc
     9001 gccttcgagt ttcacttcgg ccgtgaccag acgcgacgcg ccttttggaa gttcggggtt
     9061 ctgcgagctg gtctccgcat aggcgatcgt catgtgcttc ttcgagacgg ctgctccctc
     9121 gctcatccgc tgcccgaacc ggacgggctt cgagccgggt ttccggctca ggaaccataa
     9181 ttcgttctcc ggcccgcggc tcgtccggat gtccttgaaa cgctcggggc cgatcaggat
     9241 gtagtccccg ttcgccaggt gcataatgcg aaggaacgcg gcgcccggaa tgccgcacgt
     9301 caggcagtgg atcgcgcggg ccttcaggtc gattacaaac gcgtcgccga aactcttcgc
     9361 catgaacgcc acccgctggt tgtccggcga aatgtcggcg cgctcgccga aatgggtgag
     9421 gatttcaatg ttcgccggca ggtgatcgag cgggctgccg gtctttcgct gcgaaaaggc
     9481 ggcgcccgct agcgcgagga ccaacacggg aaggaacgga gcggtcttca taggtagaaa
     9541 ttctactcga agccggcgta ttggacttct tcttcgagtt ccagcccgaa gcgcgcccgc
     9601 acacgggact tgagttccgc gatcagttcc cgcagctcgc gggccgtgcc gcccccggcg
     9661 ttatagatca aattggcgtg atagtccgcc acccgcatgt cgccccgggc catccctttc
     9721 gccccaacct gttcgaggaa ataggcggcg gccacctttc cttcgcggac cacccctggg
     9781 gcaacctgtg cggcgacgtc ggacgggagt gcggccagca ggaggttctt gaaaatactg
     9841 ccggcgcatt tcatcgtgac cgggaatttc tcgtcgcgaa tcgcctggat ctcggacgcc
     9901 gtccgcttca gttcctccgg gtcagccggc tcgagcaaga gttcagtcga aaaaatcacc
     9961 cagtccttgc ggcgcttgaa gacgctctcc cggtaggcga actcgcagcc ggcgttgtcg
    10021 aagatgcgca cgccatgccc gtcgaagaaa cggacgctcc gaacccgctc cgagatcgaa
    10081 tgcccgtacg ctccggcgtt gccgtagaca gccgctccca cagaaccggg gattcgcttc
    10141 agcgtttcca tccctttgag gccgcgttcg attgtgaagt ccaccagcgc ttcaagttcc
    10201 gcgcccgcct ccgcgaaaac ggtgctcccc tcggcccgca gttccttcgc gctgaaccgt
    10261 aggacgagtc cgcggtaacc ctcatcggac acgatcagat tcgtgccccc gccgagcaac
    10321 aggaacggaa tgacgctgtc tcgcgccgcc tcggtggcgg cgacgaacgc ctcgacgctg
    10381 gcggtctcgg cgaacagatc cgccgggcct ccgatcccga accgcgtgta gcgggcgagc
    10441 ggaacgtcat gcagaacctg taaattggga atcgcggcga gacgcttctg cgtctccgtg
    10501 ctgggcatcg acccaattat agaggagcag gcaagatgcg taccgaattc ccgggcttcc
    10561 caccggaagc gatgagcttc ttccgtgggc tcaagcgcaa caacaaccgc gagtggttcc
    10621 aagcgcgaaa gcccatcttc gacgaaaaag tccgcgctcc catggaacgc cttgtcgagg
    10681 ctgtgaacgc cgccctgatc cggttcgcgc cggactacgt taccgaacca ggcaaggcaa
    10741 tctaccgcat ctaccgcgac acccgcttca gccgcaacaa ggttccgtac aagacacaga
    10801 tcgccgccgt tttcacgcgg cgagggttgt cgaagaacgg cagcgccggc ctttacttca
    10861 gcgtttcccc cgaggagatc gaggtcgccg gcggcgtcta catgccggat cgggaacaac
    10921 ttctggccat ccggaattgt ctcgccgggc agtacgcgga atttcgccga attctcgcgc
    10981 gcaagggtct gcggacactg atgggcggcc tctggggaga acagctatcc cgggttccca
    11041 agggatttcc cgccggccac cccgccgaag acttgctccg ttacaaacag tggcttgtgt
    11101 acgtgttgct tgaccccggt atcgccacaa cgccggacct gactggtgaa atcgtcaccc
    11161 gctttcgcgc catggtcccc tttgtcgagt tcctgaacaa acccctcagc cggaaacgta
    11221 gcgaggtcag cgtagagaac ttcttcgcat gatccgcgct ttcgtggttt acttttgcag
    11281 ccctgcagga aatcctccgg cactgccgca aggtgaaacg cttcactcgc ggggagggcc
    11341 cggcggcagt gtccttgggt aggggaaagg ttggccgccg ggaccgatac tcccgctttc
    11401 tttgtttata cgtacgagag ctggcgcggt gaggttccag acccgaagtt tttttgatgg
    11461 tgaggccggg gctatcccgc cttcagcatc cccacggccg cgttcacaag gatcgcgccc
    11521 atgccaatgg gaatcaagta cttccagcca acgttcatca actggtcata gcggaaccgc
    11581 gggaaagtcc cccggaccca gatcatcatg tagatcatcg ttcctgcttt cagcaggaac
    11641 cagaacatcc cgatcaccgc gccatttacc gccggaatca ggaacagggc ggccgccagc
    11701 aataggaaca acgccaccgc taccagcgtg accttctgga cttcgctctt caggcgcctc
    11761 accatcggga atgtagcgat tcccgaaccg gcgaatatca gcgctgggag accgtagttc
    11821 aacggatact caagccacgc cacgttcgga aacggccgca gccagcctcc caggaacaga
    11881 gtcaccgcca ccgatgcaac caccaggagg ttcgcatatt ccgccaggaa gtaaagcgcg
    11941 aagcggaaac cgctgaactc ggtgtggaag ccgcccacca attccgactc ggcctccggg
    12001 aggtcgaacg gcgagcggtt ggtttccgcc acccccccga tgacgtacat ggcgaagggc
    12061 acgatcatca gcccgaagtt gtcgaacgca aaccagacgt ggcgcgccat ctgcgccttt
    12121 acgataccca ccatgctcag cgtgcctgcc atcatgatga cggataccag agcgagcgac
    12181 aggacgactt cgtaactcac aagttgggcc gcgctgcgga gcgagccgag cagagggtaa
    12241 tgactgttgg aggcccaccc gccaaggatg attccgacaa tgcccaacgc cgacatcgca
    12301 gagatcacca gcaggccgac attgacgtcg gccgcataaa tgaagctgct gaacggaatc
    12361 accgaaaatg ccgtcagcgc ggtgaacgtg gagatcaccg gagccagcca gaagatcttc
    12421 tgatcggccc ctctcggaat cagatcctct ttgagaatca gtttcagcgc gtccgcgatc
    12481 ggttgaagca agccatgggg accgacgcgc atcggcccga gccggacctg aagatcggcg
    12541 agaaccttgc gctccatcag cgaaaggtag cccgccacca atgggaacac ggcccacacg
    12601 gccacaaaga cgatcagcag cttgaaaagc gggtgttgaa gtacggtttc cataaggtac
    12661 tcaccgcagc agcgaggtct gggccatcat gatgaacggt tgcgggtaga tgccgatccc
    12721 gagcgtcatc aagcccgtaa cgccgagcgc cagccggagt ccaaagcttg tactcagggg
    12781 cgccgcgtcc gccggttccg cgatgaacat gctcctcacc acccggaagt agtagtaaat
    12841 tgccactgcc atataaagtg ttgccaccac cgccagcacg taatgtcccg tttcgatcag
    12901 ggagaggaag atccagtatt tgccgatgaa tcctgccgtc ggaggaatgc ccgcgagcga
    12961 gagcatgaag atcagcatca acaccgcgta gccgggactc ctgtgcatca ggcccgccag
    13021 gtcgtccacg tcctccccga gcaggtcttt ccgccgcagg gccaccacca ccaggaaggc
    13081 gcccaagttc atgaacgtgt agacaagcat gtagatcagc acgcccttga tcccggtatc
    13141 gtttcccgcg atgaccccca gcagaatgta gccggcgtgg gagatcgagc tgtacgccag
    13201 catccgcttg atgttcgatt gcgtcatcgc cgcgatgttc ccgaccgtca tgctcgcgat
    13261 cgccactacc gccagcagcg gctcccacag ctcccggacg gaggccagcg gcccgaaaaa
    13321 catcctcacc agaaacgcga acgacgccgc ctttgacgcg accgaaagat acgccgtcac
    13381 ggtcgtcgga gcgccctcat aagcatccgg cgcccacatg tggaacggca ccgccgagat
    13441 cttgaacagc agcccgacgc tcgtcgtcac cagcgccagc agcaccagcg gatcccacgg
    13501 ctctcgcgac gccaccgcgt ccgcaatcgc gctcagtttc gtcgatcccg acaggccata
    13561 gaagaccgag aagccgtaaa ccagaaatcc ggtcgagaag gcccccagca gaaggtactt
    13621 catcgccgct tcgttggaac gccgctcgcc gcgcagaaag cccaccatca cgtagaagca
    13681 aagcgccatc aattccagcc cgacgaacag cgtaatcatg tcggtgccgg tggccaggaa
    13741 aaacatcccg cagttggcca aaagaatgag cccgtagtat tcgccgtgat gttcgccttc
    13801 gatctccagg tatcggtagg agaccagtgc gacgatcagc gaggctgcca ggaacaccca
    13861 gttgaagaac aatgcgaagc cgtctacggt cagtgatccg ccgaaggccg tcaactcgtt
    13921 gatcccctgc gacgcgagat aactctgctg ccgccacagc ccggtcccgg caaaaacgat
    13981 cccaagtacc acgaagatca gcagccactt ccgctgtttc gggtcgggga acaagaagaa
    14041 gtcgaacagc agcacggcgc agccgaacag cgccagcatg atggccggaa ccagaacaaa
    14101 gtgatcggtg ctggtgtaaa actggctcat cgggccgcct cctgttggac gggcatccac
    14161 gcccccggcc ggggggccgg ccggccaaga tccgttttcg cctgggcccc tccctggaag
    14221 taacctggcc gcacacgctc aaccagttgg gccaccggct tctcgagaat ctcgaagtac
    14281 ggtttcgggt agacgccaat ccagatcgac caggcgatca acggcagaaa taccgccact
    14341 tcgcgcaggc tcaggtcggg gagtttcaga ttcttggcgt tcgtcacctg gccaagcatc
    14401 gtgcgctggt agagccacag aagataggcc gcgccgagaa tcacaccggt aacggcgaac
    14461 gccgcccacg cccggttttc ttcaaacgct ccctgcaaaa tcgtgaactc gcccacgaac
    14521 ccgttcaaaa gcggcaggcc cgccgagctc agcatcgcga acgcgaacac gatggagtaa
    14581 atcggcatca cgtgcgcgag cccgccgtat tccttgatct ccctcgtgtg ccgcctctcg
    14641 taaatcacgc caacgatcaa gaagagcatc cccgtcgaga tgccatggtt gacctgctgg
    14701 atcacgctcc cggcgattcc gttcgggttg agcgcaaaaa tacccagcgt gcagaagccc
    14761 aggtggctca ccgacgagta cgccaccagc ttcttccagt cccgctgcat caggctgacc
    14821 agcgccccat agatgatgcc cacgatcgac agcccgatca gcacctgcac gatccacggc
    14881 tcaatcgacg ctttgggcag cagaggcagc gagaagcgga tgaacccgta ggtccccatc
    14941 ttcagcagca cgctggccag gataaccgag ccggccgtag gcgcctccac gtgcgcgtcc
    15001 ggaagccagg tgtggaacgg gaagacgggc agcttgatgg caaatgccac gaagagcgcc
    15061 aggaagagcc agaaggagag gctcggcccg aa
//
LOCUS       DATIFQ010000369         8566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_698477, whole genome shotgun sequence.
ACCESSION   DATIFQ010000369 DATIFQ010000000
VERSION     DATIFQ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8566
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698477"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VLE22_17840"
     CDS             <1..294
                     /locus_tag="VLE22_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16322.1"
                     /translation="RDYPAEIQVMCVGPVAFAGIPGELFVELGRKIRQESPFPYTFVV
                     SLANDDLGYIVTRESFEQGSYEPTSSRMAPGNGEMVADKAIELLRELKKQAER"
     gene            374..1387
                     /locus_tag="VLE22_17845"
     CDS             374..1387
                     /locus_tag="VLE22_17845"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HSB16323.1"
                     /translation="MKRQTPAKDKLNPKDIPAGVIGLGLMGHSIIACLLSAGHPVVGV
                     TRSLTRHRGTRRHVLALLRQMKREGLLKREPGALIGGLTLSEDYEDLARCGIVIESII
                     EDIETKKQAYRAVEEAVPADTIIGSNTSSIPLSLLQAGAAHPERFVGIHWDEPAHVTR
                     FMEIIAGEKTRPDYARRVKALAALWGKEPSLLRRDVRGFITNRVSYAMFREACHLVDS
                     GIATVEDVDRSLRNDVGWWITFAGPFRYMDLMGVEAYYRVMKDLLPELSTSPEIPSLM
                     RSVVEPGGRGISNGRGFYRYTTAQSKRWEKLFLKFNYEIRRLAMKYPEDVGNRTAARR
                     KAK"
     gene            complement(1405..2157)
                     /locus_tag="VLE22_17850"
     CDS             complement(1405..2157)
                     /locus_tag="VLE22_17850"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HSB16324.1"
                     /translation="MDRLKDKVAIVTGGANGIGRAISELFSEEGAWVLVADVEEEPGA
                     QTVSGIRQQGREAEFCRCDVSEPEDVARAVKMAAARNGRIDVLVNNAAYLSPDFHGVL
                     EASREEWERCIGVALMGTQYFTKEALPYMIAQKRGSIVNVVSIQAMVGCPTSVAYTAT
                     KSAQLGFTLSAAYDYGPHNVRVNALCPGPIQTRISPVPGAPNYDWQCGQTVLGRVGYP
                     REVAYAALFLASDEASYVTGAFLTVDGGWTSK"
     gene            complement(2169..3314)
                     /locus_tag="VLE22_17855"
     CDS             complement(2169..3314)
                     /locus_tag="VLE22_17855"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB16325.1"
                     /translation="MKISDVELIVLEAPGAYDAPEGSEEAYGVKYLGIVRVSTDAGIT
                     GYADIETQPHIARAIVEAPSEGATPGFQGLRSVLIGEDPFEVERLWHKMYMASVYYGR
                     RGAAIQVISGIDIALWDIIGKAVGKPVYKLLGGGQRDKVRAYASTLFRPDPASMADAC
                     RHYVDQGFTAVKFGWGVFGQDRDRDVLLVEAARRALGDRVDLLVDTGWYVHRTAKEAI
                     GVVRRLEPFKPFFIEELLSPEDYDGYAQVATAVDTLIACGEQESTEWGFQMLIERGKV
                     DVVQPDISRCGGLTTARKIVHMTELYNRLCVPHAWISDLLTAASLHLNAFMRRSVFVE
                     FNVTSGPLSREMCRNPLRMKDGYVDVPQGPGLGVEVDESAIVKYRVG"
     gene            complement(3295..4167)
                     /locus_tag="VLE22_17860"
     CDS             complement(3295..4167)
                     /locus_tag="VLE22_17860"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB16326.1"
                     /translation="MIVDCHSHVFAYPGHLSDEFVREANLRSRGHPIDLNVTSDKHWK
                     AMAGVDKVIVFGMRAFHSGIFTPNEYIAEYAALHPDKVIGFAGVDPSVDDVRETLEHA
                     IGKLKLRGVKLGPVYQNVHPTDPRMMAVYEFCQARRVPIMIHQGTTFPRKAPSKYALP
                     ILLEDVALAFPDLKMVIAHLGHPWIDDTLVLIRKQPNFYADISALHYRPWQFYNALIT
                     AKEYGVLDKLLFGSDYPFTTPEASIDALRNFNRLVEGTNLPRLAPDDIERLIASPTLE
                     YLGLEQTNREDFRC"
     gene            4270..5298
                     /locus_tag="VLE22_17865"
     CDS             4270..5298
                     /locus_tag="VLE22_17865"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HSB16327.1"
                     /translation="MPEVIYDPASIDFDRPGKHHYQLAFHLDSSWGYSLVPLTIINGL
                     RVGSGEAPPGVAAFGGTHGNEWEGQVAVKRLCQDLDPDVMRGRVILIPQLSESACIAN
                     TRVSPLDGVNMNRAFPGNPRGTVSFRISHFVKTRIFPLVRVVLDIHSGGYEGGFAVPT
                     TSFHPIPDPVQRAEIAMVAELFDPPFIMIYSSQMASGLLTDEAEAEGKITVGGEFGYG
                     ESVNPKGTLHAYEGIRNVLRHYGIIEGEVVKIDPRRESRPRFIEAANLEDYVPCPANS
                     IWEPVVELGADVAKGDLLGRLHDFADHSSEPIEIWAHRSGVVAMLHMPAVSRKGDTLF
                     VICRDAQL"
     gene            5335..5808
                     /locus_tag="VLE22_17870"
     CDS             5335..5808
                     /locus_tag="VLE22_17870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSB16328.1"
                     /translation="MTAGHAYMLLSLVSFSLIGIFAKVADGRDCRPAALYALVYFWAL
                     ALVSAFAFGLRGVTAGAPAQVYWIALPFGMCAAVAGIAFQIGIRYGKISTSWLVINLS
                     AAIPTVGSILIYREPVSARKLVVISLVVISILLLWKDKQVEDARNASRLPAGEEG"
     gene            5812..6231
                     /locus_tag="VLE22_17875"
     CDS             5812..6231
                     /locus_tag="VLE22_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16329.1"
                     /translation="MWLRMMLIAFVTNGLGPFGLKILAEANLSQQFRYQYLAAWYGGG
                     FLLGLVALLGSKVRPRWDEAVIAGVMGLGSFGGQLFSALALEANVPGHIVFPVTTGGN
                     LFLVSAAGLLLFRERIGGYGVAGILTGIVSLVLLSMS"
     gene            6286..7011
                     /locus_tag="VLE22_17880"
     CDS             6286..7011
                     /locus_tag="VLE22_17880"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB16330.1"
                     /translation="MKRFAVVAASATLLLITGGSRMSGDSVPEYRSLFNGKDLTGWVN
                     VNTDPDTWSVRDGVIVCKGQPIGVMRSKEQFENFILHIEWMHTEPGGNSGVFVWSNAN
                     PDPKNRLPNGVEVQMLELDWVNLHKLNGEKPPLAYVHGELFGVGGVDTIPDNPRGPRS
                     KSVENRCKGRGEWNTYDVVAVDGVIKLAVNGKFVNGISRSTQKKGYLCLESEGAEIHF
                     RNIRILELPPSVTSPYQIAPALE"
     gene            complement(7041..7117)
                     /locus_tag="VLE22_17885"
     tRNA            complement(7041..7117)
                     /locus_tag="VLE22_17885"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7081..7083),aa:Pro,seq:tgg)
     gene            complement(7172..>8566)
                     /locus_tag="VLE22_17890"
     CDS             complement(7172..>8566)
                     /locus_tag="VLE22_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16331.1"
                     /translation="NLYRFLEQIRWTHDQIRKWEPSKLLTVGSPFYAFHPAFWTAVDE
                     EELADSGITGVVLEENYEIDSLMPEYLHALAGGKPVMDFEHHGVIHQILPSFLHGDAA
                     ISMWWWNDRKRWTPNEPINEWASSFPQSYTIPLSDVGKAMRDALDLRRLGQDIAALGS
                     APRPVVLLYSKTSMLQQVPDKSREMTSFPYLSGLRRIYNASQSAGLYVGLTTEKKILA
                     GDLRQRRVLVLPGAEFVPGPVTDAILRWAEEGGTVVVSPDSLLADEYARPSRTLQSLG
                     LRLARREPPRLKRGEWFVTQYNLADLARMALRKDGEKPLSADGVALEAAGGKQILEGG
                     RSMVAARFEDGSPALLRVPRGRGVVYWLASPLDPASWGRFLAMVAADSGLKPELRVTR
                     EGGGLAPEVEYRVTAFEGRRLAYLYNDSDRDVTLTLAPAFPFKRIADRRAEAPLAGRR
                     LALPARETAILEFR"
BASE COUNT         1598 a         2674 c         2704 g         1590 t
ORIGIN      
        1 cgcgactacc cggcggagat ccaggtgatg tgcgtcgggc cggtagcgtt cgccggtatt
       61 ccgggagaac tgttcgtcga actcgggcgc aagatccgcc aggagtctcc gttcccgtac
      121 accttcgtcg tttcgctggc gaacgacgat ctcggataca tcgttacccg cgaatcgttc
      181 gaacagggca gctacgagcc gacaagctcc cgcatggctc cgggaaacgg ggaaatggtc
      241 gcggacaagg cgatcgagct actgcgagag ctcaaaaagc aggccgagcg ctgaatccgg
      301 aagtcccaag attccattga caatttcggc gaccgcgtga tagcgttggc tccgcgaaac
      361 ggaacaggac cccatgaagc gccagactcc tgccaaagat aaactgaatc cgaaagacat
      421 ccccgcaggc gtgatcgggc tcggactgat gggccacagc atcatcgcct gtctcctgtc
      481 ggccgggcat ccggtggtgg gcgtgactcg cagtttgacg cggcaccggg gaacacgccg
      541 ccacgtgctg gccttgctga ggcagatgaa gcgggagggt ttgctgaagc gcgagccggg
      601 ggcactcatc ggaggtctta cgctctcgga ggactatgag gacctcgcgc gctgcggcat
      661 cgtcatcgag tcgatcatcg aggacatcga aacgaagaag caggcgtatc gcgcggttga
      721 ggaagcggtg cccgcggaca ctatcatcgg cagcaacacg tcgtcgattc cgctgagtct
      781 ccttcaggcc ggcgccgctc accccgaacg gttcgtcggc atccattggg acgagccggc
      841 gcacgtgacg cgcttcatgg agattatcgc gggagagaag acgcgaccgg attacgcgcg
      901 gagagtgaag gcgcttgccg ccctgtgggg taaggagccg tcgctgctgc gccgggacgt
      961 gcgcgggttc atcacgaatc gggtgtcgta tgcgatgttc cgcgaagcgt gccacctggt
     1021 ggattccggc atcgccacgg tggaggacgt ggaccggtcc ctgcgaaacg atgtgggctg
     1081 gtggattacc ttcgccgggc cgttccgcta catggacctg atgggcgtcg aggcctacta
     1141 ccgcgtaatg aaggatctgc tcccggaact ctcgacgagc ccggagatcc catcgctgat
     1201 gcggagcgtg gtggagccgg gcgggcgcgg aatttcgaat ggtcgtggtt tctaccggta
     1261 caccacagct cagtcgaagc gctgggagaa gctcttcctg aagttcaact acgagatccg
     1321 gcgactggca atgaagtatc cggaggacgt cggcaaccgg accgccgcgc ggcgcaaggc
     1381 gaagtagcgg cgccgaagtc gcgtctactt cgatgtccag cctccgtcga ccgttagaaa
     1441 cgcgccggtg acgtaggagg cttcatcaga ggccagaaac agtgccgcat aggcgacttc
     1501 acgcgggtag ccgacgcgcc ccaacaccgt ctgaccacat tgccagtcat agttgggcgc
     1561 tccgggtact ggcgagatcc gggtctgaat tggccccggg cagagcgcgt tgacgcggac
     1621 attgtgcggg ccatagtcgt acgctgcact gagagtgaaa ccaagctgcg ctgacttggt
     1681 cgccgtgtac gcgacagagg tggggcagcc taccatcgcc tgaatcgaga cgacattcac
     1741 gatcgagcct ctcttctggg cgatcatgta cggcaacgcc tccttggtga agtactgggt
     1801 ccccatcagc gcgacgccga tacaccgttc ccactcctcc cggcttgcct ccagcacacc
     1861 gtggaaatcg gggcttaaat aggcggcgtt gttcacaagg acgtcgatgc ggccgttccg
     1921 cgcggccgcc atcttcaccg cgcgcgcaac gtcttcaggc tcggacacgt cacaacggca
     1981 gaactccgct tccctgccct gctgccggat gccggacact gtctgagctc cgggctcctc
     2041 ctcaacatcc gcgaccagaa cccacgcacc ttcttcagag aacagttcac tgattgcccg
     2101 gccgatgccg ttggcgccgc cggtgacgat ggcaaccttg tctttcaacc tgtccatatc
     2161 caaagtccct atcccacgcg gtatttcacg atagcgctct catccacctc gacaccgaga
     2221 ccaggaccct gcggcacgtc aacgtagcca tccttcattc gcagcgggtt gcggcacatc
     2281 tcccggctca gcgggccgct ggttacgttg aactcgacga agacggaccg ccgcataaac
     2341 gcattcaagt ggagcgaggc ggccgtcagg agatcggaaa tccaggcgtg gggaacgcac
     2401 agccggttgt acaactcggt catgtggacg atctttctcg cagtggtaag accgccgcag
     2461 cgcgaaatat ccggctgaac gacgtccact tttccacgct cgatcagcat ctggaagccc
     2521 cattcggtcg attcctgctc gccgcaggcg atcagcgtgt cgaccgcggt ggcaacttgc
     2581 gcgtagccgt cgtagtcctc cggcgagagc agctcctcga tgaagaacgg cttgaacggt
     2641 tcgagccgac gcacgacgcc gatcgcttcc ttggctgtgc gatgaacgta ccagccggta
     2701 tcgaccagca ggtcaactct atctcccaac gcgcggcggg ccgcctccac aaggagaacg
     2761 tcgcggtcgc ggtcttgccc gaacacgccc cagccgaact tcacggccgt gaagccctga
     2821 tctacgtaat ggcggcaggc gtccgccatt gacgcggggt ccgggcggaa cagcgtgctc
     2881 gcataggccc tgaccttgtc gcgctggcct ccgccgagaa gcttgtacac cggcttgcca
     2941 acggcttttc cgatgatgtc ccagagtgcg atgtcgatgc cggagatcac ctggatcgcg
     3001 gcgccgcggc gtccgtagta gacggacgcc atgtacatct tgtgccagag ccgctcgact
     3061 tcgaacgggt cctcgccgat cagaacggac cgcagtccct gaaatcccgg ggttgcaccc
     3121 tccgagggcg cctccacaat cgcgcgtgcg atgtggggct gggtctcgat gtcagcgtat
     3181 ccggtgatcc cggcgtcggt actgacccga acaatgccga gatacttgac cccgtacgcc
     3241 tcttcggaac cttccggcgc gtcataggcg cccggagcct ccaggacgat cagttcaaca
     3301 tcggaaatct tcacggttgg tttgctccaa tccgaggtac tccagtgtgg ggcttgcgat
     3361 aagccgctcg atgtcgtccg gcgcgaggcg gggcagattg gttccttcca caagcctgtt
     3421 gaagttccgc agcgcgtcga tggaagcctc gggcgtggtg aacgggtagt cggagccgaa
     3481 cagtaacttg tctagaacgc cgtactcttt cgcggtgatc agggcgttgt agaactgcca
     3541 cgggcgatag tgaagcgccg aaatgtccgc gtagaaattg ggctgcttgc gaatcagcac
     3601 cagcgtgtcg tcgatccacg gatggccgag gtgcgcaatg accatcttca agtccgggaa
     3661 tgccagagcg acgtcttcga gcaggattgg caaggcgtac ttcgacggcg ccttcctggg
     3721 aaatgtcgtt ccctggtgaa tcataatcgg cacgcggcgg gcctggcaga attcatacac
     3781 cgccatcatg cgcggatcgg tggggtgaac gttctggtag accggtccca gcttgacccc
     3841 gcgcagtttt agctttccaa tcgcatgctc gagcgtctcg cggacgtcgt ccaccgacgg
     3901 atcgaccccg gcgaaaccga tgaccttgtc cggatgcagc gcggcgtatt cggcgatgta
     3961 ctcgtttgga gtgaatatcc cagagtgaaa cgcgcgcatc ccgaatacga tcaccttgtc
     4021 cacacccgcc atggctttcc agtgtttgtc ggatgtgacg ttcagatcga tcggatgccc
     4081 gcgcgaacgc agattcgcct cgcgtacgaa ctcgtccgat agatgcccgg ggtaggcgaa
     4141 cacatggcta tggcagtcaa cgatcacgca atctctcctc cgtcggaatg cctcggacac
     4201 tgtagcacgc cgggaaacgg cccgaaaagt ggcgcgcccc tcggccgtgg tagtctcatc
     4261 aatgctccca tgccagaagt catctacgat cctgcatcca tcgattttga ccgtcccggg
     4321 aagcatcact accagcttgc gttccacctc gattcgagct ggggctactc gctggttcca
     4381 ttgacgatca tcaacgggtt gcgcgtcggt tccggcgagg cgccgcccgg agtggccgcg
     4441 ttcggcggga cgcacggtaa cgagtgggaa gggcaggtgg ccgtcaagcg gctctgccag
     4501 gacctcgacc cggacgtaat gcgtggccgg gtgatcctga taccgcagtt gagcgagagc
     4561 gcttgcatcg ccaacacgcg ggtatcgccg ctcgacggtg tgaacatgaa ccgtgcgttc
     4621 ccgggtaatc cccgtggaac ggtctctttc cggatctccc atttcgttaa gacacgaatc
     4681 ttcccgctgg tgcgggtggt tctcgacatc cactcgggtg gttacgaggg gggattcgcg
     4741 gttccgacga cttcatttca tccgatcccg gacccggtcc aacgagccga gatcgcgatg
     4801 gtggccgagc tgttcgatcc cccgttcatc atgatctact cgagccagat ggcttccggg
     4861 ctgctgaccg acgaagccga ggccgaaggc aagatcaccg tcggcgggga gttcggctac
     4921 ggcgagtcgg tcaacccgaa gggaacgctg cacgcgtatg aggggatcag aaacgtgctg
     4981 cggcattacg gaatcatcga gggcgaagtg gtgaagatcg acccgcggcg cgaaagccgg
     5041 ccgcggttca tcgaggccgc caacctggag gattacgttc cctgccccgc caacagcatc
     5101 tgggagcccg tggtggaact gggtgcggac gtggcgaagg gcgatctgct cggccgcctc
     5161 cacgacttcg ccgaccattc ctcggaaccg atcgagatct gggcgcatcg ctcgggggtg
     5221 gtcgcgatgc ttcacatgcc cgcggtctcc cgaaaagggg acacgctgtt tgtcatctgc
     5281 cgtgacgccc aactgtaagg ctggggaccc gaaacgggcc gggagattga acgaatgacc
     5341 gccggacacg cttacatgct cctctcgctg gtgagcttca gcctcatcgg catcttcgcg
     5401 aaggttgcgg acgggagaga ctgccggccg gcagccctgt acgctctggt ctacttctgg
     5461 gcacttgcac tggtttctgc gttcgctttc ggcctcaggg gcgtgaccgc tggagcgccg
     5521 gctcaggtgt actggatcgc gttgccgttc gggatgtgcg ccgccgtggc cggtatcgcc
     5581 ttccagattg ggattcgata cgggaagatc tcgacaagct ggctcgtcat caacctttcg
     5641 gctgcgattc cgactgtcgg ctcgattctg atttaccgcg agccggtgag tgcacggaag
     5701 ctggtggtga tctccctggt ggtgatctcc atcctgcttc tgtggaagga caagcaggtg
     5761 gaggacgccc gcaacgcaag ccggcttccc gccggggagg agggctgagt catgtggctg
     5821 cggatgatgc tgatcgcgtt cgtcaccaac ggcctgggcc cgttcggctt gaagattctc
     5881 gccgaagcca acctctcgca gcagttccga taccagtacc tcgccgcctg gtacggcggc
     5941 ggtttcctcc tcggtctagt cgcgctgctg ggtagcaagg tccggccgcg gtgggacgag
     6001 gcggtgatcg caggagtgat ggggctgggc agtttcggcg ggcagctctt ctcggccctg
     6061 gcgctcgagg cgaacgtgcc cgggcacatc gtctttccag tgacgaccgg agggaatctg
     6121 ttcctcgtgt ccgcagccgg cttgctattg ttccgggaga ggatcggggg ctacggcgta
     6181 gccggtatcc tgactggaat tgtttcactc gtcctgctga gcatgagctg accgccggtg
     6241 aaaacgatcc ggttttccgg taagctccta agaatgcgat ccacgatgaa gcgctttgcg
     6301 gtggtggcgg cgtcggcgac ccttctgctg ataactggcg gcagccgaat gtcgggcgat
     6361 tccgttccag agtaccggag cctgttcaac ggcaaggacc tgaccgggtg ggtcaacgtg
     6421 aacaccgacc cggacacatg gtccgtccgg gacggcgtga ttgtctgcaa agggcagccg
     6481 atcggggtga tgcgctcgaa ggagcagttc gagaacttca ttcttcatat cgagtggatg
     6541 cacaccgagc cgggtggaaa ctcgggagtg tttgtctgga gcaacgccaa tccggatccg
     6601 aagaatcgcc tgccgaacgg cgtcgaggtc cagatgctgg aattggattg ggtgaacctg
     6661 cacaagctta acggcgagaa gcctcctctc gcgtacgttc acggcgagct gtttggcgtc
     6721 ggcggggtgg acaccatacc cgacaatccg aggggtccgc ggagcaagtc cgtcgaaaac
     6781 cggtgcaagg gccggggtga gtggaacacc tacgatgttg tggcggtgga cggcgtgatc
     6841 aaactcgcgg taaatggcaa gtttgtgaac ggcatcagcc gctcgacaca gaagaaaggg
     6901 tatctgtgtc tcgaatcgga gggcgctgag atccactttc gcaacatccg gatcctggag
     6961 ttgccgccca gcgtaacttc cccctaccag atcgccccgg cgctggaata ggcatcgagc
     7021 cgtagagccc gtcgacgaaa tggtcggggc ggggcgattt gaacgcccga ccccctgcgc
     7081 ccaaggcagg tgcgctacca ggctgcgcta cgccccgaca ctgttgctac tttgatggta
     7141 agccacttcg ccggcggaag ccaacctcgc ctcagcgaaa ttcgaggatc gccgtctccc
     7201 tagccgggag cgcgagccgg cggccggcca gcggcgcttc ggcgcgccgg tccgcgatgc
     7261 gcttgaacgg gaacgccggc gctagcgtga gcgtcacgtc ccggtccgaa tcattgtaga
     7321 gatacgccag ccgccgtccc tcgaacgccg tcacgcggta ctcgacttcc ggcgcgagac
     7381 ctcctccctc gcgcgtcaca cgcagctccg gcttcagccc cgagtccgcc gccaccattg
     7441 cgaggaaccg tccccaactc gccggatcga gcggtgatgc cagccagtaa accacgccac
     7501 gccctcgcgg cacgcgcaac agcgcgggag aaccgtcctc gaaccgcgcc gccaccatcg
     7561 agcggcctcc ctcgaggatt tgcttgccgc ccgccgcttc gagcgcaaca ccatccgcgc
     7621 tgagcggctt ctcgccatcc ttgcgcagtg ccatcctcgc caggtcggcc agattgtact
     7681 gggtcacaaa ccattctccg cgcttcagcc gcggaggttc ccgccgggcg agccgcagcc
     7741 cgagggactg gagggtccgc gacggccgcg catactcatc ggcgagcagc gaatccggtg
     7801 aaaccaccac cgtcccgccc tcttccgccc agcgcaggat cgcatccgtt accggcccgg
     7861 gaacgaattc ggccccgggg agcaccagca cacggcgctg ccgcaggtca cccgcgagga
     7921 tcttcttctc cgtggtcagc cccacataca ggccggcgga ctgcgacgcg ttgtagatcc
     7981 tccgcagccc ggagaggtag ggaaacgacg tcatctcgcg ggacttgtcc ggcacctgct
     8041 ggagcatcga cgtcttagag tagagcagca ccaccggacg cggcgcgctc ccgagcgcgg
     8101 cgatgtcctg gccgagccgc cggagatcca gcgcgtcccg catcgccttg cccacgtcgc
     8161 tgagcgggat cgtatagctc tgcgggaacg acgacgccca ctcgttgatc ggctcgtttg
     8221 gcgtccaccg cttccggtcg ttccaccacc acatggaaat ggcggcgtcc ccgtgcagga
     8281 agctcggcag aatctggtgg atcacgccgt ggtgttcgaa gtccatcacc ggcttcccgc
     8341 cggccagtgc atggaggtat tcgggcatca gcgagtcgat ctcgtaattc tcctcgagga
     8401 ccacgccggt gatccccgaa tccgcaagct cttcttcgtc tacggcggtc cagaacgcgg
     8461 gatgaaacgc atagaacgga gagccgaccg tgagcagctt cgaaggctcc cacttccgga
     8521 tctgatcgtg cgtccagcgg atctgctcca ggaagcggta gaggtt
//
LOCUS       DATIFQ010000370        23676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_506572, whole genome shotgun sequence.
ACCESSION   DATIFQ010000370 DATIFQ010000000
VERSION     DATIFQ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 23676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23676
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506572"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..223)
                     /locus_tag="VLE22_17895"
     CDS             complement(59..223)
                     /locus_tag="VLE22_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16332.1"
                     /translation="MTLGTARVSKRFLGKDRGEMGTDYHFAVVAKWLSVPHFSVPMHE
                     FRRVKDHDSA"
     gene            complement(220..1683)
                     /locus_tag="VLE22_17900"
     CDS             complement(220..1683)
                     /locus_tag="VLE22_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16333.1"
                     /translation="MRALILLLASGLFAVAADPIRLHPANPHYFLFRGKPVVLVTSAE
                     HYGAVLNPDFDYRKYLETLSADGMNYTRIFAGSYVEKPGAFGIKRNTLAPAQGRFLPP
                     WARTSTPGYAGGGAKFDLDRWSGEYFTRLKSFVREAGKRGIVVEVTLFCSSYRDEHWN
                     LSPLNPANNVNGTSAIDWRKLNTLDNGNILERQEQMVRKIVRELNEFDNVFFEIQNEP
                     WADAPVKVETISPYPTDSALSRYPKTVEVAGDASLAWQRRIASVIVPEEAALPKRHLI
                     AQNYCNSLYAVREVAPEVSILHFHYAYPEAVTWNYRWNKLIGYDESGFLGNSDSVYRR
                     QGWDFLLAGGGLFNNLDYSFTVGREDGTDTEPNGPGGGSPVLRKQLRVLSDFIHSLPF
                     LEMAPDNAVVRKSPGAVTHALSAPGKAYAIYLTGSSPCTLALDVPRGRYRAEWINTKT
                     GRTDRDEKFDHPGGLRELASPPFEEDVALRLAQEPEK"
     gene            complement(1740..3032)
                     /locus_tag="VLE22_17905"
     CDS             complement(1740..3032)
                     /locus_tag="VLE22_17905"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSB16334.1"
                     /translation="MSDKEPVVNMAAEPVRKEPLPLEFPGVHHIDEREVEAAVRVLRS
                     RSLFRYYGIDLQKEVESFEEEFARFTGAPYAVAVASGTGALHTALAALGVGPGQEVIV
                     PAYLWVSVLAAVVNLGAIPVLADIDDTFCLNPVEVEKQITSRTRGIILVHMSGAPGDA
                     AAIAALARAEGLWLLEDCAQCAGGSIGGRKVGTFGDMGIYSFQMNKNMTAGEGGAIVT
                     SDPRLHQRAFACHDLGYRRDDSGRLVFDEPDMRLWGRGYRLDELRAAVLRVQLEKLPN
                     TIAAMRRSKYRIREALERFPEKVALRRIQDPEGDTGCFLITTYPEAETARKVNTALRA
                     EGIVTWPQGVSNVVMTDWGLHLYYNNVSLVEKGSVDPKGFPWSLAENAELARNYAKGA
                     CPVADSLFERSILLPIPSCLTEKDEDDIITAFRKVLDK"
     gene            complement(3050..5389)
                     /locus_tag="VLE22_17910"
     CDS             complement(3050..5389)
                     /locus_tag="VLE22_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16335.1"
                     /translation="MKIRSAIVLLSICLAGLCLAAQGGAPSAREEGGLWVAANEGLEI
                     AVDKNTGAIRRLLDRTSNEDYCNQKVSGAVPAREFEVGERVAGITLYDELANREFSDL
                     AENATVTAARSGNAGGSVSLSVDKTFPGADFTVTETFTLSADHLRWDVRLKKTQGKDR
                     TVRVIQFAPLPLGRYRGWAPISDSIPMKPYTPWAIEYGQSVSGAVGEARWRTNIPMVV
                     FYSRENKRAISFASAFEVPAVRVRFLNNTGATADFHWNSRHYPMRERPYLQVSNEYLG
                     LRDNRNIETSLLISVHPSDWRPALGWVYERYRKYFDADPKFDKWDGVYASQGELLKDA
                     LTENDRRKIVEGRRDRGVKWEEQHGHFPWYGLMIPKPDVETWVCDSHLPREQTTLSRD
                     KIRRHHRRNLEFGIGTFIYYNITEAEYWYAEKEFPESIARDESDRPVGAFRAAEYPDK
                     RACYLMNADPVSPFGQHMIRQAKELVEAYPDLAGFFWDVYGRSYMFDFAHDDGITMVN
                     NKPAYYPLFMYERMMRDHVGPLLRSKGMTFTANKPTTITACEGLDGIMVIENTPEEET
                     PHWIAAQSFLGLNRHMMMLDGASGTHAELFFQDCLRYGLFYSDLGATDEKRRPLPPEQ
                     VARNKEIEKIYAPFIQRLKGKKWIFHPEALELPENTDGNIFRLPDGSVMVTVTSPWRI
                     LRKSTEADNDLEVICRLPDAASMTNVYVSAVDLRETAKVTAEHSGDMVKIVVPRHGRS
                     TVILLSAKPDKTLEATAVRRDPGAAQKKKKRDASTSDDY"
     gene            5486..6601
                     /locus_tag="VLE22_17915"
     CDS             5486..6601
                     /locus_tag="VLE22_17915"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HSB16336.1"
                     /translation="MRSIIALLIILLAPVHVAGVEKVPLIHTTDLFHPPADPDDHLDL
                     ATLFALDEFDIRAVLLDRAVMQSTAAGEPDREPGFVPVVQLCYLTGRSTPVAAGPAAP
                     LKAPVDPATDRPRREQAAIDLLLRALRESPEPVVVTVLGSARIVTAAFNRDPDLLRRK
                     VRSVVVNAGSSGDDASEYNVMIDVAAYVGLFRSGLPIDWYPCAAPGPNRSVALNTADR
                     NSYWKAAHADLFRGLPQPLLGWFVHGFAANGRGDLLRALREQGRGSAGAMLMQGERHM
                     WSTASMALAAGRVLAKTPEGWRFVPAAKAPAGVEMQLLALDPVSVTISDDGKTKWQPA
                     PDGSHIRLFRRHAGPGHTAAVTEALNALLRSLPVDPL"
     gene            complement(6703..7815)
                     /locus_tag="VLE22_17920"
     CDS             complement(6703..7815)
                     /locus_tag="VLE22_17920"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSB16337.1"
                     /translation="MPSRVFMALPLLVLLPGAITGAGIEQTELFRGGEGAYHTYRIPA
                     LVVTKKGTLLAFCEGRKGSASDSGNIDLLLRRSFDSGRTWTPAQVVADFGDDTIGNPA
                     PVVERKTGAILLLLTRNPGHVTEAQIVDGTAGGTRTVWLSRSTDDGATWSAPVEITTS
                     VKRPGWTWYATGPGNGIQLSSGRLVVPCDHITLDEKVMHSHVIYSDDGGKSWSLGGVA
                     GEKTNESAVVELSDGTLLLNMRSYHGRNRRAVAHSRDGGLTWSDTSLAEELIEPVCQA
                     SLIRLAPGSKNDGRLLFSNPADVKRVRMTVKLSLDEGSTWPAARLVHEGPSGYSSLAV
                     LRDKSIGLLYERGDTRYYERITFARFTLKWLTEVTK"
     gene            complement(7821..10550)
                     /locus_tag="VLE22_17925"
     CDS             complement(7821..10550)
                     /locus_tag="VLE22_17925"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HSB16338.1"
                     /translation="MAYTIPGRRRRLNWKVILLIVLFVLLFGGRFLANYFIEYQWWKE
                     LGQVPTWIDMLLYGLAPVVAASLLSFVALWLIHARALKFAGTSLRSHPGYAVVSSIAL
                     LFLAIFVASSTIDTWAVVRYFGGRNLPPEATQWRDSVFGLPLRFYLFDLPFYSILRRY
                     VLFLSVFGAIIYWVAARGWQLFQRLPELRETQEFDPRMLRLEGALESRFLRGLAVVFL
                     LALAFQFYLGRYEMLLNDHGFMVGIDYVDEKIGLPLQWLVIAACLAAAVLVSAGKWKI
                     AALMALAFVARGVVPPAVYALYVRPNEISIQQPYIRTHIHATRSAFGLEEHTREIEFK
                     AKLETRIDPAKHKPLLDNVRLWDWRAFHDTVTQIQALRPYYVFADSDVDRYIIDGNLR
                     QMLLTPRELDIRQLRDAEARWINPHFIYTHGYGMVMAEANRITTDGLPLLFVQDAPPE
                     VKTASLKLTRPEIYYGEVVHEPVFVRTAQKEFSYPSGADNVFTRYDGLGGFPVSSMIT
                     RVAAALREGDFNILLTSYLTPESRMMIRRNVRHRLATVANFINWDTDPYLVLSDSGRL
                     VWTVDGYTTSAAHPYSAGTRLPDGVEANYIRNSVKATVDAYDGTTRLYIFDPADPIIQ
                     AYQRIFPGLFLPASKMPADLRAHARYPETYFNAQAEIYRTFHMLDPQAFYNKEDLWDI
                     ARNIQGQDARPKALAPTYVVATLPGETKPEFLLIVPFTPRNKDNMIGLMVARCDGPHL
                     GELVFLQLSKQELMFGPMQIEARINQDQNISKDLSLWNQQGSQVLRGQILTLPVEDTI
                     LYVEPIYIQASEARMPQLKKVVLAMGNLLIYEDTYEQALAKLGGYQLPAAPAQPSATA
                     GTPATPAPVASELDRRLGLVRQHLRRYRELAGQGRWSEAGKELEALEAETQRR"
     gene            complement(10617..11897)
                     /locus_tag="VLE22_17930"
     CDS             complement(10617..11897)
                     /locus_tag="VLE22_17930"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HSB16339.1"
                     /translation="MTRLLIKNGRVIDPSARRDETGDVLIENGAIAAAGPNLDATGAE
                     IIDATGMVVAPGFVDIHVHLREPGFEHAETIETGSRAAAAGGFTSICPMPNTAPVNDN
                     PTVTNYVVERARRFAAVNVFPIGAITKNSAGEELAAIGSMKNAGIVAISDDGRPVMNA
                     RVMRRAMETARSFDLPVIDHCEDLNLSAGGDMHEGLYSTRLGLRGIPGSSEDVMVARD
                     VLLAESTGARIHVAHISSARSVALVASARTRGVAVTCEATPHHISLTDAELASYDSDY
                     KMKPPLRTAGDVAAVVEGLVSGTVDAIATDHAPHPGSEKMQEFEKCPFGVIGLETALG
                     VALEVLYHSGKIGLPRLVELLTSAPARILRLNRGTLSVGAPADVTIFDTETPWTYDVN
                     RSLSKSKNTPFHGRTFRGGPVATIVNGAIVWRRE"
     gene            complement(11900..12808)
                     /locus_tag="VLE22_17935"
     CDS             complement(11900..12808)
                     /locus_tag="VLE22_17935"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HSB16340.1"
                     /translation="MAAGLLGIEGLGRDEIEAILTRAKDFQPMQDHAFKRLDVLRGRM
                     IVNLFFEASTRTRTSFEIAAKRLGADAISITAAGSSVSKGESLVDTLNTLAAMRPNAI
                     VMRHAASGAPLFLARHLPIPIINAGDGTHEHPTQALLDARTILDNLPSLEGLRVAIIG
                     DIAHSRVARSNIHLLAKFGVDIVLCGPASLVPAELAQIAPGVRISYDMCEAIRDADVI
                     MMLRVQLERQHETAFPAGEYFQFYGLRLEHLQLAKPNVLVMHPGPINRGREIASEVAD
                     SQRSVILNQVENGVAVRMAVLERILG"
     gene            complement(12812..13366)
                     /gene="pyrR"
                     /locus_tag="VLE22_17940"
     CDS             complement(12812..13366)
                     /gene="pyrR"
                     /locus_tag="VLE22_17940"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HSB16341.1"
                     /translation="MALTREKTQLMTASEIDRTLVRLAHEVLEKSRDLDRLAFVGIRR
                     RGVPLAMRLATKIRDLEKREVPVGILDISLYRDDLSTIADKPVVNNTDIPFDVTGKDI
                     ILMDDVLYTGRTIRAALDALFRHGRPARVQLLVLIDRGHRELPIEARYVGRMVQTTDR
                     EIIEVKFREIDELEKVLLVERTDG"
     gene            13438..15780
                     /locus_tag="VLE22_17945"
     CDS             13438..15780
                     /locus_tag="VLE22_17945"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HSB16342.1"
                     /translation="MKYAASVRTVLLFCFYLLFAGAATGVVEPKRPMSPEQELRVREE
                     ARSIVVSGETFAYTFSKESGLISSVQILGNVITDGTPIPDLILAEHLDRSFSQYTARR
                     ETQARLSVASAGPAHVVVAAEGAYTAQDGRRFPLRYSITYDISIDGVILVAVRNSAVE
                     DCTFRWLTLSGGAVKSELAKFINWMPDQAGSQSTGYLFRPLSEIKEAKILGGVWLPWI
                     WLGDQNLGLEVTTWDVGSQTYNNVDGTSRSDQHEMFNVRREGAGVRWENWMIGGSTIY
                     AKKGWQRGGEFALAVTPSKKFDPYYAMIKGAHLGPHQHRLSVNLPDENQLRILAQNGY
                     NLVVGMANWRSGEYVPLNDADLRRTISLCHKFGMKVIPYITLVDLSHATAAFRDHGEE
                     WAIEPTTENYRLTPRFRDLKVEMAYRNDPEQETTLMCPASEGWRGFWKKQIDRIISDY
                     DFDGLYVDFWFGRMACENTRHGCGGHFRKATVLGSREMLAYAYNRIKAKNPRAILKAN
                     TNTLATSLITSVIDLRLVGEGTDVTRMDPNSRQWLYTSHRLGEPTEFLWASTRWNPAQ
                     KAGFAALINFLPQYYERPPFEPRNSFDDFDVFRFLEAEKGDWYLGISGHQRLPVTPAQ
                     VKANVVERGGTTLVTLVNTEESPVAAVVPAKQNVLVYDPLGERLLEAASGALKVDLTS
                     GGYRHIVMTGRPSQPKLLFALGVRRPASEEWDAGARRLRFSVEGVDGARIRFAVFSPE
                     RPTAVLNGRGDKAPFEWTAESRLVRFEAPHASGERFEIYY"
     gene            15797..18130
                     /locus_tag="VLE22_17950"
     CDS             15797..18130
                     /locus_tag="VLE22_17950"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HSB16343.1"
                     /translation="MKSDLICLFGVFAACAATAATLEIKQPLNTGQELKVWQDSAVIV
                     VSGETFAYTFSRQNGLITSVRVLDREITDGTPIPDLVVAEQLDRNTSPYLARQETRAS
                     VSVTTTGPSRVVITAEGAYTGEHGTSFPLRYALSYDIAIDGVALVSVRNSAVGDCGFR
                     WLALSGGAVKSEYAKFINWMQEQSGSQSTRYLFRPLSEVKEEKVLGGTWLPWIWLGDQ
                     DRGLEVTTWDVGAQTYNRIDSTRRQDQNEVFTVRRAGSGVRWENWLVNRTFVYVRKGW
                     QRGGEFALAVTPSKKFDPYYSMVKGAHLGPHQHEQTLKLPDEGQIRTLAQNGFNLVVG
                     MANWRSGEYIPLNDADLRRTIELCHKYGMKIIPYITLVDLSHATEAFRDHGEEWAIEP
                     TTEYSKSGSRIFRRDPRGEAAYRNDPEQETTLMCPGAAGWREHWKKQVDRFLREYDFD
                     GLYIDFWYGRMVCENTRHGCGGRYRKHTVLGSRDMLTYAYNRIKSKDPRAILKANTNT
                     LATALITSLAELRLVGESIDMTTLDSDSRQWLYSSYRLGEPTEFLWDRTRWDPARKNG
                     FAALVNFLPQVYDRPPFEPRKTFDDFDVFRSFEAEKGRWYLGIGGHGRLTATPSGVSV
                     NLVEREAARLATVINPAEAPVTASIPVKPGTLAYEPLAERLLDTAGGAVAVEIAPGGY
                     RHMILMESPERPYLLAALGARRPLPQQWDGQARRLRVSAEGVEGARIRYAVYSAERPK
                     ELVGARGARVPFEWTAETRLVRFETSHSSAEVFELRF"
     gene            complement(18138..19406)
                     /locus_tag="VLE22_17955"
     CDS             complement(18138..19406)
                     /locus_tag="VLE22_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16344.1"
                     /translation="MRLSTLLLFAALGAGAQQLREVRVPFAGKELTYVCAASASAAGP
                     APLLVLLHPDTGADLAAIRRFATNWQGAPARRRWHMIVPWAKATGPAINDAGVEATEA
                     IVEDAKVRFQVDPGRVYLAGIGEGGSQVFYLASRVPDLWTAAVAVEGSPRAAMASNRL
                     FGANTSLVPILWSVRLSDHATALPLSQTLTTAGYNVQLQSSAGIAIDHVLEWLAKHRR
                     EEFPRKVDCESGDNRFGRCYWVEMTRFDPSLRNDVLRSTRVPPGTRAFLSIGSFGFNP
                     SAPGPGVLIQYLPPGFKGPLTQGDRIVALAGREIRDGQHYMKMLEEMKEEKPVALMIE
                     RAGKRQRVETRIVLPKREEINTARIQVEYVPNKDEIQVLSRGVAEARMTLPQNWAPVA
                     VNWNGNDAGRITKPGCWVISLSAPARPCVP"
     gene            complement(19420..19737)
                     /locus_tag="VLE22_17960"
     CDS             complement(19420..19737)
                     /locus_tag="VLE22_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16345.1"
                     /translation="MKIRARCPRTVTLLAFMALLAAGQIAPPGQEPEPRLPSGKSQRE
                     AILKEEYAKTLADAGRLVELAESLKIELEKNDRHVLSVSSLKKAEELEKLAKRIRNRL
                     KRF"
     gene            19796..21100
                     /locus_tag="VLE22_17965"
     CDS             19796..21100
                     /locus_tag="VLE22_17965"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSB16346.1"
                     /translation="MDDVNVGIVGLGNVGMGTLGILAGNAEQIALKLGFRLRVRAVCS
                     RTAGRKTLPAGLGDVFITTDWRELVSHPEVDIVAELVGGTGVAAEIIEGAITNRKSVV
                     TANKELMALCGAEIWDRAIRAGINLAMEASVAGGIPIHAVLREGISGDRIVALYGILN
                     GTSNYILTEMEKRSASLPDVLAEAQRLGFAEADPSADIDGWDARSKLAILAALAFGEK
                     ITPSDIFTEGIRRISQVDFEYARLLRHTIRLVCGAQQTHDGLILYVRPALIPLSTILA
                     GVQGAYNAVWVKGYYGADTFYYGRGAGPNPTGVAVVSDLMRVAREIRNGSPERVSPFA
                     HERLGEYTPIAVIRQKRAYFLRFRVVDRPGIIAALAGILAEKKISLEAVLQLPCAGKQ
                     DVPFVITVEPTSEQSVREAVEEMSGLDFLLEPPLALPMEPGL"
     gene            21202..22593
                     /locus_tag="VLE22_17970"
     CDS             21202..22593
                     /locus_tag="VLE22_17970"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HSB16347.1"
                     /translation="MLRLLRELIPVSVLTLLVSEFVLYGSCFVLASFIVLEVDPTVFL
                     LYDGGLGRIVVAVLSILLGLYFHDLYSQFLVKSRVLLLQQSCQVIGIAFLIQALLSYG
                     SADWMLPRWLMMLGSGLTLVAMLAWRITYSSVVLRAMGEARVLFLGDNSLVREMVAQI
                     AEQPELGLKVVGYLDEGLSPDCDWQGATRLGSVELLRDVAGQVKPDRIVVGMRERRQR
                     LPVSDLLDLRLSGVRVEEAATMFEAICKRIALKDLRPGQFIFSADLGPRPGSILFRSA
                     YSGLVALTGTLLTLPLMAAAAIAIKLTSAGPVLFRQTRVGLHGRPFTLYKFRSMYQDA
                     EVSTGAVWATRDDPRVTPLGRWLRRLRLDELPQFFNVLRGEMVIVGPRPERPEFVSTL
                     TDEVPYYGQRNWVKPGITGWAQINHKYGDTVEDSVKKLEYDLYYIKNMSLALDLFIIF
                     HTVKTMLLSRGAQ"
     gene            22670..22744
                     /locus_tag="VLE22_17975"
     tRNA            22670..22744
                     /locus_tag="VLE22_17975"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22702..22704,aa:Gly,seq:tcc)
     gene            22777..22852
                     /locus_tag="VLE22_17980"
     tRNA            22777..22852
                     /locus_tag="VLE22_17980"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22810..22812,aa:Thr,seq:tgt)
     gene            22903..22991
                     /locus_tag="VLE22_17985"
     tRNA            22903..22991
                     /locus_tag="VLE22_17985"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22937..22939,aa:Tyr,seq:gta)
     gene            23025..23095
                     /locus_tag="VLE22_17990"
     tRNA            23025..23095
                     /locus_tag="VLE22_17990"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            23106..23181
                     /locus_tag="VLE22_17995"
     tRNA            23106..23181
                     /locus_tag="VLE22_17995"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23139..23141,aa:Thr,seq:ggt)
BASE COUNT         4295 a         7300 c         7482 g         4599 t
ORIGIN      
        1 cagaccgatg gcaagcgcat cgttggattc gaagacactc gcgagttcgg gaggggagtc
       61 atgcggagtc atggtctttg acgcggcgga actcgtgcat cggcacggag aaatggggga
      121 cagacagcca tttcgcaaca acggcgaaat ggtagtcagt ccccatttct ccccggtctt
      181 ttcccaaaaa ccgcttgctg acgcgcgcgg ttcccaaagt catttctccg gttcctgcgc
      241 caggcgtagc gcgacatcct cttcgaaggg cggtgaggcg agttcgcgaa ggccgccggg
      301 gtggtcgaat ttctcgtcac ggtcagttcg tccggttttc gtgttgatcc actcggcgcg
      361 gtagcggccg cgcggcacgt ccagtgcgag ggtgcatggg gaactgccgg tgagatagat
      421 cgcgtacgcc tttcccgggg ccgacagtgc gtgcgtcacc gcaccgggcg atttccggac
      481 cacggcgttg tccggggcca tctccaggaa gggcagggaa tggatgaaat cgctcagcac
      541 cctgagctgc tttcgaagca cggggctgcc gccgccaggg ccgttcggct cggtgtccgt
      601 gccgtcctcg cgcccaacgg tgaaggagta gtccagattg ttgaacagac cgccgcccgc
      661 aagaaggaag tcccacccct gccggcgata gacggaatcc gaattcccga ggaacccgga
      721 ttcgtcgtag ccgatcagct tgttccatct gtagttccac gtcaccgcct cggggtaggc
      781 gtagtggaag tgaaggatcg acacctcggg cgccacttcg cggacagcat agagggagtt
      841 gcagtagttc tgcgcgatca gatgccgttt cgggagagcc gcttcctcgg gcacgatcac
      901 cgacgcgatg cggcgctgcc aggcgagcga cgcgtcgccg gcaacttcca ccgtcttcgg
      961 atagcggctg agtgccgagt ccgtcggata ggggctgatc gtctcgacct tcaccggagc
     1021 gtccgcccaa ggctcgttct ggatctcgaa gaagacgttg tcgaactcgt tcaactctcg
     1081 aacgatcttc cgcaccatct gttcctggcg ctcgagaata ttcccattgt cgagggtgtt
     1141 cagtttgcgc cagtcgatcg cagacgtgcc gttgacgtta ttggccgggt tcagcgggct
     1201 cagattccaa tgctcgtccc gatagctgga acagaaaagg gtgacctcga cgacaattcc
     1261 acgcttgccg gcctctctga caaagctctt caggcgggtg aagtattcgc cgctccagcg
     1321 gtcgagatca aacttcgcgc cgccgccggc gtagccgggc gtcgaagtcc gcgcccacgg
     1381 cggcaggaac cggccctgcg cgggggcgag tgtgttccgc ttgatcccga atgcacccgg
     1441 cttctcgacg taagaacccg cgaagatgcg cgtgtaattc atcccgtccg cggaaagcgt
     1501 ttcgaggtat ttccggtaat cgaaatccgg gttcagaacg gcgccgtagt gttcggccga
     1561 agtcaccagc acaaccggct tgccgcggaa caggaagtag tgcgggttgg cggggtgcaa
     1621 ccggatcggg tccgcagcga cggcgaacag tcccgaggcc agcagaagga tcagagctcg
     1681 catcggacta atcctccacg ccagcgcggg agcgctgcgc ctgcgaaaac ctctacgatt
     1741 catttgtcaa gcaccttgcg gaacgccgtg atgatgtcgt cctcgtcctt ttccgtgagg
     1801 cacgacggaa tcggcagaag gatacttcgc tcgaacaggc tgtcggcgac gggacaggcg
     1861 cccttggcgt agttccgcgc aagctccgcg ttttcggcga gcgaccaagg gaagcctttg
     1921 ggatcgacgc tccccttttc gaccaggctg acgttgttgt agtagaggtg caggccccaa
     1981 tccgtcatca ccacgttcga cacgccctgc ggccaggtga cgatgccctc ggcgcgaagc
     2041 gccgtgttaa ctttccgcgc cgtctccgcc tcggggtagg ttgtgatcag gaagcagccg
     2101 gtgtcaccct cgggatcctg gatccggcgg agcgcaacct tctccggaaa gcgctccaat
     2161 gcctcgcgga tgcggtactt gctgcggcgc attgcggcga tcgtgttggg cagcttctcg
     2221 agctgcacgc gaaggacggc cgcacgcagc tcgtcgaggc ggtagccgcg gccccagagc
     2281 cgcatgtcgg gctcgtcgaa gacgagccgg ccgctgtcgt cccgccggta gccgaggtcg
     2341 tggcaggcga acgcgcgctg gtggagcctc gggtcggagg tcacaatcgc gcctccctcg
     2401 cccgccgtca tgttcttgtt catctgaaag ctgtagatgc ccatgtcgcc gaaggtgccg
     2461 acttttcggc caccgatgct cccgccggcg cactgggcgc agtcctcgag cagccagagg
     2521 ccctccgcgc gcgcgagcgc agcgatggcg gccgcgtcgc cgggcgcccc gctcatgtga
     2581 acgaggatga tgccgcgcgt ccgggacgtg atctgcttct ccacttccac cggattgaga
     2641 cagaacgtgt cgtcgatgtc ggcgagcacg gggatggcgc cgaggttcac caccgctgcg
     2701 agcacggaca cccataggta ggccgggacg atcacttcct ggcccggtcc gacgccgagc
     2761 gcggcaagcg ctgtgtgcaa cgctccggtt ccactcgcca ccgcgacggc gtacggcgct
     2821 cccgtgaaac gcgcgaactc ctcttcgaac gactcgacct ccttttgcag atcgatgccg
     2881 tagtaccgga agagggagcg cgagcgaagc acacggacgg ctgcctcaac ctcccgttcg
     2941 tcgatgtgat gcactcctgg aaattcaagc ggcagcggtt ccttccgtac cggttctgcc
     3001 gccatattga cgactggttc tttgtcgctc atcttcgatc ctttttgatt cagtagtcgt
     3061 ctgaggtact tgcgtcgcgc ttcttcttct tttgggccgc gccggggtcg cgccggacgg
     3121 ccgtagcttc cagggttttg tccggtttgg ccgagagcag gattaccgtg gaacgcccgt
     3181 gtcgaggcac gacgatcttc accatgtcgc ccgagtgctc ggccgttacc ttggcagtct
     3241 cgcgcaaatc gaccgccgag acatagacat tcgtcatcga cgccgcgtcc ggcagccggc
     3301 agatcacctc caggtcgtta tccgcttcgg tcgacttgcg cagaatccgc cacggcgaag
     3361 tcaccgtgac catcacgctg ccgtccggca ggcggaaaat attgccgtcg gtattctcgg
     3421 gcaattccag cgcctcggga tggaagatcc acttcttgcc cttcagccgc tggatgaacg
     3481 gcgcgtagat cttctcgatc tctttgttgc gggctacctg ttcgggcggc agcgggcggc
     3541 gcttttcgtc cgtcgcgccg aggtccgagt agaacagccc gtaacggagg caatcctgga
     3601 agaacaattc ggcgtgggtg ccggaagcac cgtcgagcat catcatgtgc cggttcagcc
     3661 cgaggaagct ctgtgcggcg atccagtggg gggtctcttc ctcgggggtg ttttcgatca
     3721 ccatgatgcc gtcgaggccc tcgcaggcgg tgatggtggt cggcttgttc gccgtgaacg
     3781 tcatgccctt gctgcggagc agcggaccga cgtggtcacg catcatccgc tcatacatga
     3841 acaacgggta gtaggccggc ttgttgttga ccatggtgat cccgtcgtcg tgcgcgaagt
     3901 cgaacatgta gctgcggccg tagacgtccc agaagaagcc ggcgaggtcg ggataggcct
     3961 ccaccagctc cttcgcctgg cggatcatgt gctggccgaa cggcgacacc gggtcggcgt
     4021 tcatcaggta gcaggcgcgc ttgtcgggat actctgcggc gcggaaagcg ccgaccggcc
     4081 ggtccgactc gtcgcgggcg atgctctccg ggaactcctt ttcggcgtac cagtattcag
     4141 cttcggtgat gttgtagtag atgaaagtgc cgatgccgaa ctccagattt ctgcggtggt
     4201 gccgccggat tttgtcgcgg ctcagggtcg tctgctcgcg gggcaggtga gagtcgcaga
     4261 cccaggtttc aacgtccggc ttcgggatca tgaggccata ccacggaaag tggccgtgct
     4321 gttcctccca tttcaccccg cggtcgcgcc tcccttcgac aatcttccgc cgatcgttct
     4381 cggtaagcgc gtccttcagc agttctccct gcgaggcata gacgccgtcc cacttgtcga
     4441 acttcgggtc ggcgtcgaag tacttccgat agcgctcata gacccatccg agcgcgggcc
     4501 gccagtcgga gggatgcacg gagatcagca ggctcgtctc gatgttgcgg ttgtcccgca
     4561 gcccgaggta ctcgttgctg acctggagat acggccgctc acgcatcgga tagtgccgcg
     4621 agttccagtg gaaatcagcg gtcgctccag tgttgttcag gaaacggacg cgaacagccg
     4681 gcacctcgaa ggcggaagcg aacgagatag ctcgcttgtt ctcccgcgag tagaagacga
     4741 ccatcgggat gttcgtgcgc caccgtgctt cgccgaccgc acccgagacc gactggccgt
     4801 attcaatcgc ccatggggtg tagggtttca tggggatcga atcggagatt ggcgcccagc
     4861 cgcggtagcg gccgagtggc agcggagcga actggatcac acgtactgtc cggtccttgc
     4921 cctgggtttt cttcagccga acatcccagc ggaggtgatc cgcgctaaga gtaaacgttt
     4981 ccgtgacggt gaaatccgcg ccggggaacg tcttgtcgac cgagagagat accgaacctc
     5041 cggcattgcc ggagcgggcc gcagtgacgg tggcgttctc cgcgaggtct gaaaactcgc
     5101 ggttcgccag ctcatcgtac agcgtgatcc cggcgacgcg ctcgccaact tcgaattcgc
     5161 gggcaggtac cgcgccactc accttttgat tgcagtagtc ctcgttgctg gtgcggtcga
     5221 gcaggcgacg gatggcgccc gtgttcttgt cgacggcgat ctccaggcct tcgttggcgg
     5281 cgacccaaag gccgccttcc tccctcgccg agggagcgcc gccctgcgct gcgaggcaga
     5341 ggccggcaag acagatgctc aggagtacaa tagcgctgcg aatcttcatg agagttcctg
     5401 tgtttcggat tgtcgcgtcc cagattatcg cccgctctat gcacagtcaa ggtaaggcga
     5461 cacacgcgga gtacgataca ggtctatgcg ttccatcatc gctctgctca tcatcctgct
     5521 cgctcccgtt catgtggccg gagtggagaa ggttccgctg attcacacga cggacttgtt
     5581 ccatccgccc gccgatcccg acgatcacct tgatctcgcg actctattcg cgctcgatga
     5641 gttcgacatc cgggcggtgc tgttggatcg agccgtcatg cagtcgactg cggcgggaga
     5701 accggatcgc gaacccggct tcgtaccagt cgtccagctt tgctacctga cgggccggag
     5761 cactcccgtg gctgccggtc cggcggcgcc attgaaagcg ccagtggacc ccgcgacaga
     5821 ccgtccccgc cgcgagcagg cggcgatcga tctgctgctg cgagcgcttc gagagagccc
     5881 ggagccggtg gtcgtgacgg tactcggcag cgcgcgcatc gtgaccgcgg cgttcaaccg
     5941 cgatcccgac cttctgcgcc gcaaagtgag gagcgtcgtt gtgaatgcag gctcgagcgg
     6001 agatgacgct tcggagtaca acgtcatgat cgacgtggcg gcgtacgtgg ggctattccg
     6061 cagcggtctg ccgatcgatt ggtacccctg cgccgcgccg ggaccgaatc gttccgttgc
     6121 gctgaatacc gcggaccgga attcgtattg gaaagccgcc cacgcggatc tgttccgcgg
     6181 cctgccgcag ccgctgctcg gatggttcgt ccacggcttt gccgcgaacg gccgcggcga
     6241 tcttctgcgc gcgttgcgcg agcagggtcg aggctccgcc ggtgcgatgc tgatgcaggg
     6301 agagcggcac atgtggtcga cggcgtcgat ggcgctggcg gcagggagag tgctagcgaa
     6361 gaccccggaa ggatggcggt tcgttccggc ggcgaaggcg cccgctggcg tcgaaatgca
     6421 gctactggcg ctcgatccgg tttcggtcac catcagcgat gacggtaaga cgaaatggca
     6481 gcccgctccg gacggcagcc acatccggtt gttccggcgg catgccggtc ccgggcatac
     6541 tgccgccgtt acagaggccc tgaacgcgct gctacgctct ctgccggtgg acccgctgta
     6601 ggcttgagct actcagtcca gggttctgcg gacggaccaa ttctcaggaa gtgcggagga
     6661 actcgcctgg tgtgacaacg gcgggaaaaa tctctcggag cctcatttcg taacctcagt
     6721 gagccatttc agcgtgaatc gggcgaatgt gatccgctca tagtaacgcg tgtcaccccg
     6781 ttcgtagagg aggccgatcg acttatcccg cagcaccgcc agcgaagagt atccggacgg
     6841 gccttcgtgg acaagccgtg ccgccggcca ggtgctgcct tcgtccagac tcagcttcac
     6901 tgtcatcctg acccgtttca cgtcggctgg attcgaaaac agcaggcggc cgtcgttctt
     6961 cgagcccggc gcgaggcgga tcaggctggc ttggcagacg ggctcgatca actcctcggc
     7021 taacgaggta tcggaccacg tcagcccgcc atcgcggctg tgggcgaccg cgcgccggtt
     7081 cctgccgtgg taactccgca tgttcagcaa cagcgtgccg tcgctcagct cgacgaccgc
     7141 cgattcgttg gttttctcgc cggccactcc gcctaggctc cagctcttgc cgccgtcgtc
     7201 gctgtagata acgtgtgagt gcatcacttt ctcgtccagc gtgatgtgat cgcaggggac
     7261 cacgagacgg ccgctcgaaa gctggatccc attgccggga ccagtggcgt accacgtcca
     7321 tccgggccgc ttgacggagg tggtgatctc cacgggcgca ctccaggtcg ccccgtcgtc
     7381 cgtactgcgg ctgagccaga ctgtccgcgt gccgccggcg gttccatcca cgatctgcgc
     7441 ttcggtgacg tggccggggt ttcgtgtcag tagcaacagg atcgcgccgg tcttgcgttc
     7501 gaccactggc gccgggtttc cgatggtgtc atcgccgaag tcggcgacca cttgcgcggg
     7561 cgtccatgtc cggccgctgt cgaagctcct tctaagcaac aggtcgatgt tgccgctgtc
     7621 actcgccgag cccttccggc cctcgcagaa cgccagaagg gtacctttct tcgtcacgac
     7681 cagcgcagga atgcggtagg tgtggtacgc tccttcaccg ccgcggaaca actccgtctg
     7741 ctcgatgccg gcgcccgtga tggcgccggg cagaagcacc agtagcggca gagccatgaa
     7801 aacgcggctc ggcatctcgt tcacctccgc tgggtctctg cctccagcgc ttcgagctcc
     7861 ttaccggctt cagaccaccg accctgacct gcgagttcgc ggtagcggcg caggtgctgc
     7921 ctgacgagac ccaggcggcg gtcaagctcg gaggcgacag gcgcgggagt cgccggagtc
     7981 ccggccgtcg ccgacggttg agcgggagcg gcaggcaact gatagcctcc gagtttcgcc
     8041 agcgcctgct cgtacgtatc ctcgtagatc agcaggttac ccatggcgag caccaccttc
     8101 ttaagctgag gcatgcgcgc ctcggacgcc tggatgtaga tcggctcgac atagagaatc
     8161 gtgtcttcca caggcagggt caggatctgg ccgcgcaaga cctgggaccc ttgctggttc
     8221 cacaggctca ggtccttcga gatgttctga tcctggttga tgcgggcctc aatctgcatc
     8281 ggtccgaaca taagctcctg cttggagagc tggagaaaga cgagttcgcc gaggtgcgga
     8341 ccgtcgcaac gcgccaccat cagcccgatc atgttgtctt tgttgcgcgg ggtgaacgga
     8401 acgatcagca gaaactcggg tttcgtttcg cccggcagcg tcgcgaccac ataggtcgga
     8461 gccaacgctt tgggacgggc gtcctgcccc tgaatgttgc gcgcgatgtc ccagaggtcc
     8521 tccttgttgt agaacgcctg cgggtccagc atatggaacg tccggtagat ctccgcttgc
     8581 gcgttgaagt aagtctccgg gtagcgcgcg tgggcgcgga ggtcggcggg catcttggat
     8641 gccggcagga acagtcccgg gaagatccgc tggtacgcct ggataatcgg gtcggccggg
     8701 tcgaagatgt acagccgggt agtaccgtcg taggcgtcca cggtcgcttt caccgagttg
     8761 cggatgtagt tcgcctcaac tccgtccggc agccgggtgc ccgccgaata cggatgggcg
     8821 gccgaggtgg tgtagccgtc cacggtccag accagccggc ccgaatcgga gaggacgagg
     8881 tacgggtccg tatcccagtt gatgaaatta gccaccgtcg ccagccggtg ccgcacgttc
     8941 cggcggatca tcatccggct ttcgggcgtc aggtacgacg tcagcaggat gttgaaatca
     9001 ccctcgcgca aggctgccgc gacccgcgtg atcatcgagg agaccgggaa tccgccgagt
     9061 ccgtcgtatc tcgtgaacac gttgtcggcg cccgagggat agctgaactc cttctgtgcc
     9121 gtacggacga acaccggctc gtgaaccacc tcgccgtagt agatttccgg gcgggtgagt
     9181 ttcaggctgg ctgtcttgac ctcgggcggg gcgtcctgaa cgaacaggag gggtaggccg
     9241 tcagtggtga tccggtttgc ctccgccatc accatcccgt agccgtgcgt atagatgaag
     9301 tggggattga tccatcgcgc ctcggcatca cgaagctgcc ggatgtcgag ttcgcggggc
     9361 gtcagcagca tctgccgcag gttgccgtcg atgatgtacc ggtccacatc gctgtcggcg
     9421 aagacgtagt agggccgcag ggcctggatc tgcgtgactg tgtcgtggaa ggcccgccaa
     9481 tcccagagcc gcacgttatc cagcagcggc ttgtgcttgg ccgggtcgat ccgcgtttcc
     9541 agtttcgctt tgaactcgat ctcccttgtg tgctcttcga gcccgaaagc gctgcgggtg
     9601 gcatgaatat gggtccggat atacggctgc tggatcgaga tttcattggg ccgtacgtag
     9661 agagcgtaaa cggccggcgg cactactcca cgggcgacga acgccagcgc catcagcgcg
     9721 gcgatcttcc atttcccggc cgagacgagg accgccgcag ccagacaggc ggcgattacc
     9781 agccactgga gaggcaggcc gattttttcg tccacatagt cgatgccgac catgaagccg
     9841 tgatcgttca gcagcatctc gtagcggcca agatagaact ggaacgccag cgcgagcagg
     9901 aacaccaccg caagcccgcg cagaaagcga gattccagcg cgccctccag ccgcagcata
     9961 cgcgggtcga actcttgcgt ctcccgcagc tcaggcaggc gctgaaatag ctgccatcca
    10021 cgggcggcca cccagtagat gatcgctccg aacaccgaca gaaaaagcac gtagcgccgc
    10081 agaatggagt agaacggcag gtcaaacaga tagaaccgaa gcggcagtcc aaagaccgaa
    10141 tcgcgccact gagtcgcctc gggtggcaga tttcgtccgc cgaaatagcg tacgaccgcc
    10201 cacgtatcaa tcgttgacga cgcaacgaag atggcgagga agagcaacgc gatgctggaa
    10261 actacggcgt agccagggtg cgaccgcagg cttgtcccag cgaactttaa cgctcgcgcg
    10321 tgaatcagcc acagcgccac gaacgacaga agactcgccg ctactacggg tgcgagcccg
    10381 tacagcagca tgtcgatcca tgtgggcacc tggcccaact ccttccacca ttggtactca
    10441 atgaaatagt tggcgaggaa tcgtccgccg aatagcagaa cgaagagaac gatgaggaga
    10501 atgaccttcc agttcaagcg gcgacgtctg ccggggatcg tgtaagccat gcctactctt
    10561 ttagcagagc gggcgagaca gcgcgagcgc gacgtggggc cgtgagtaga gatcgcctac
    10621 tcgcgcctcc agacaatcgc gccgttgacg atcgtcgcca ccgggccgcc acggaaggtg
    10681 cggccgtgga atggggtgtt cttgctcttg gagagcgagc ggttcacgtc gtacgtccaa
    10741 ggcgtctccg tgtcgaagat ggtgacgtcg gccggcgctc cgacgctcag tgtgccccga
    10801 ttcagccgga gaatgcgcgc gggagcggag gtgagcagct ccaccaggcg cggcagaccg
    10861 atttttccgc tgtggtagag gacttccaga gccacgccaa gcgcggtttc caggccgatc
    10921 acgccgaacg ggcacttctc gaactcctgc atcttctcgc tgcctggatg gggcgcgtgg
    10981 tccgtggcga tggcgtccac ggttcccgag accaaacctt ccacaaccgc tgcgacgtcg
    11041 cccgccgtcc gcagcggcgg cttcatcttg taatcgctgt cgtaggaggc cagttcggcg
    11101 tccgtgagcg agatgtgatg cggagtggcc tcgcacgtca ccgccacacc gcgcgtcctt
    11161 gcactggcca cgagagcgac cgaccgcgcc gacgagatgt gggccacatg aatacgcgct
    11221 ccggtggatt cggctagcag gacgtcgcgg gccaccatca cgtcttccga cgacccggga
    11281 attccgcgca ggccgagacg cgtcgagtag aggccttcgt gcatgtcgcc gccggcgctc
    11341 agattgagat cctcgcagtg gtcgattacc ggaaggtcga aggagcgagc cgtttccatc
    11401 gcgcgccgca ttacgcgtgc gttcatcacc ggtcgcccgt cgtcggaaat ggctacgatg
    11461 ccagcgtttt tcatcgagcc gatggcggcg agttcctcgc cggcgctgtt ctttgtgatt
    11521 gctccaatcg ggaacacgtt gacggcagcg aagcggcgcg ccctctcgac aacgtagttt
    11581 gtgaccgtgg ggttatcgtt gaccggggcc gtgttcggca tggggcagat cgacgtaaac
    11641 ccgccggccg ccgccgcgcg ggatcccgtt tcgatcgtct ccgcgtgctc gaaacccggc
    11701 tcgcgcaggt gcacgtgaat atcgacgaag ccgggagcga ctaccatgcc ggtggcgtcg
    11761 atgatttcgg ctcccgtggc gtccagattc ggaccggctg cggcgatcgc gccgttttca
    11821 atcagtacgt cgccggtttc gtcccgccgc gcggagggat cgatgactcg cccgttcttg
    11881 atcagtagcc tggtcatggt caacccaaaa tccgttccag caccgccatt ctcacggcga
    11941 cgccgttttc cacctggttg agaatcaccg accgctgtga gtcggctact tcggaagcga
    12001 tctcccggcc gcggttgatc ggaccggggt gcatcaccag gacgttgggc ttcgctagtt
    12061 gcaggtgctc cagacgaagc ccgtagaact ggaagtactc gccggccggg aacgccgtct
    12121 catgctgcct ctcgagttgc acgcgcagca tcatgatcac atccgcgtcg cggatggcct
    12181 cgcacatgtc gtaactgatc cgcacccctg gggcgatctg cgccaattcg gcgggtacga
    12241 gcgacgctgg accgcacagc acaatatcga cgccgaactt cgcgaggaga tggatgttgg
    12301 agcgggcgac ccggctgtgg gcgatgtcgc cgataatggc cacccgaagc ccttcgagtg
    12361 aaggcagatt gtcgaggatt gtgcgggcgt cgagcagcgc ctgcgtcggg tgctcgtgcg
    12421 tgccgtcacc ggcgttgatg atcggaatcg gcagatgacg tgccagaaag agcggcgcgc
    12481 cggaagccgc gtgccgcatg acgatggcgt taggccgcat cgccgccagc gtgttcagag
    12541 tgtccacgag cgactcgccc ttagaaacgc ttgaacccgc ggcagtgatc gagatggcgt
    12601 cggcaccaag ccgtttcgcg gcgatctcga aactcgttcg cgtccgcgtc gaggcttcga
    12661 agaataagtt gacgatcatc cggccccgca ggacgtccag ccgcttgaag gcatggtcct
    12721 gcatgggctg gaaatccttg gcccgggtca ggatcgcttc gatctcgtcc cgtccgagtc
    12781 cctcgattcc caatagcccg gctgccatag ttcacccgtc cgtccgttcc accagaagaa
    12841 ccttctccag ttcgtcgatt tcccggaact tcacttcgat gatctctcta tccgtggtct
    12901 gcaccatgcg gccgacgtag cgcgcctcga tcggaagctc gcggtgaccg cggtcgataa
    12961 gcacaagcaa ttgcacacgg gccggccgtc cgtgccggaa caatgcgtcg agcgccgcac
    13021 gaatggtccg gccggtgtag agaacgtcgt ccatcaggat gatgtccttg cccgttacgt
    13081 cgaacgggat atcggtgttg ttcacaaccg gcttgtctgc gatcgtcgaa agatcgtcgc
    13141 ggtagagact gatgtcgagg atgccgaccg gaacctcccg cttctcgagg tcgcgaatct
    13201 ttgtcgccag ccgcatcgcc agcggcacgc cccgccgccg gatgccgaca aaagccaatc
    13261 tgtccagatc gcgtgatttt tcaagtactt cgtgcgccag ccggactagc gtccgatcga
    13321 tctcggacgc cgtcatcaac tgggtcttct cgcgcgtgag agccatgccg cccctttttc
    13381 caaacgctac agggtatcac aacggtcaca ggctgtctgt ggaagaatgt gaatgccatg
    13441 aaatacgccg catccgtccg gaccgtcctg ctgttctgtt tctatcttct cttcgccggt
    13501 gccgccacgg gagtggtcga gcctaaacgg ccgatgtcgc cggagcagga actcagggtc
    13561 cgtgaggaag ctcgctccat tgttgtgtcg ggcgagacgt ttgcttacac cttcagcaag
    13621 gagagcggcc ttatcagctc ggtacagatc ctcggtaacg tgatcaccga tggcaccccg
    13681 atcccggatc tgattctcgc cgagcacctt gaccgctcgt tttcgcaata caccgcccgc
    13741 cgggagacac aggcccgctt gtcggttgct tctgccggcc ctgcccacgt ggttgtcgcc
    13801 gccgagggcg cttatacagc acaggacggc aggcgcttcc ccttgcgcta ctcgatcacg
    13861 tacgacatct cgattgacgg tgtgatcctg gtggccgtcc gtaattctgc cgtcgaagac
    13921 tgcacgttcc gctggctcac cctctctggc ggagccgtaa agtcggaact ggcaaaattc
    13981 atcaactgga tgccggacca ggcgggttcc caatccacgg ggtacctttt ccggcccctc
    14041 tcggaaatca aggaggccaa gatcctcgga ggcgtgtggt tgccttggat ctggctcggg
    14101 gatcagaacc tcggcctcga ggtcaccacg tgggacgtcg gctcgcagac ctacaacaac
    14161 gtcgacggca cttcgcgcag cgatcagcat gagatgttca acgtgcgccg ggagggcgcc
    14221 ggcgtccgct gggaaaactg gatgatcggc gggtcgacga tttacgcgaa gaagggctgg
    14281 cagcgcggcg gagagttcgc tcttgcggtg acaccgtcga agaagttcga cccgtactat
    14341 gccatgatca agggcgcgca tctgggtccg caccagcacc gcctcagcgt gaacctgcca
    14401 gatgaaaacc agcttcgaat cctggcgcag aacggctaca acctcgtcgt cggtatggca
    14461 aactggcggt ccggggaata cgtaccgctc aacgatgcgg atctccggcg cactatcagc
    14521 ctctgccaca agttcggaat gaaggtgatc ccatacatca ctctggtcga cctctcgcat
    14581 gcgaccgcgg cgtttcgcga ccacggcgag gagtgggcta tcgagccgac caccgagaac
    14641 taccgcctca ccccgcgctt ccgcgacctg aaggtcgaaa tggcctaccg caacgacccg
    14701 gagcaggaga cgacgctgat gtgtcccgct tcggagggct ggcgcggctt ctggaagaaa
    14761 caaatcgatc ggatcatcag cgattacgac ttcgacggcc tctacgtgga cttttggttc
    14821 ggacggatgg cctgcgagaa cacgcggcac gggtgcggcg gccatttccg aaaagccacg
    14881 gtgctcgggt cgcgtgagat gctggcgtac gcctacaacc gcatcaaggc gaagaacccg
    14941 cgcgcgatcc tcaaagccaa cacgaacacc cttgccacct cgctgatcac cagcgtgatc
    15001 gacttgcggc tggtcggcga gggaacggac gtcacgcgca tggacccgaa cagtaggcag
    15061 tggctgtaca cttcccaccg gctcggtgag ccaacagagt tcctgtgggc atcgacgcgg
    15121 tggaatccgg cgcaaaaggc gggtttcgcc gcgctgatca acttcctgcc ccagtactat
    15181 gagcggccgc cgtttgagcc aaggaacagc ttcgacgact tcgacgtctt ccgcttcctc
    15241 gaggccgaga aaggtgactg gtacctcgga atcagcggcc accagcgcct gccggtcaca
    15301 cccgctcagg tgaaagcgaa cgtcgtggag cggggtggta cgacattggt cacactcgtc
    15361 aacaccgaag agtcgccggt agcggccgtc gttccggcga agcaaaacgt cttggtatac
    15421 gatccgcttg gcgagcgtct actcgaagcg gccagcggag cgctgaaagt cgacctcacg
    15481 tccggaggct atcgccacat cgtgatgact ggccgcccgt cacagccgaa gctgttattc
    15541 gcactcggcg tccggcgtcc tgcctcggag gaatgggacg ccggggctcg acggctgcgg
    15601 ttttcggtgg aaggcgtcga cggcgcccgc attcgcttcg cggtcttcag ccccgagcgt
    15661 cccactgccg tcctcaacgg acgaggcgac aaggcgccgt tcgaatggac agccgagagc
    15721 cgtctcgtcc gtttcgaggc gccccatgct tcgggcgaga ggttcgagat ttactactga
    15781 ggttgggcaa actgtcatga agagcgatct gatctgtctg ttcggtgtct ttgccgcgtg
    15841 cgccgccact gcggctactc tggaaatcaa gcaaccgctg aacaccggcc aggaattgaa
    15901 agtctggcag gattccgctg tgatcgtcgt gtcaggcgag acctttgcgt acacgttcag
    15961 ccggcagaac ggtcttatca cctccgtgcg ggtgctcgac cgtgaaatca ctgacgggac
    16021 gcccatcccc gacctcgtcg tcgccgaaca gctcgacaga aacacgtcgc cctatctggc
    16081 ccgccaggag acgcgggcga gcgtgtcggt gactacaacc gggccctcgc gggtcgtgat
    16141 cacggccgag ggcgcttaca cgggggagca cggcacgagt tttccgcttc gctacgctct
    16201 cagttacgac atcgccatcg acggcgtggc gctcgtctct gtccggaatt cggcggtcgg
    16261 ggactgcggc ttccgctggc ttgcgctctc cggaggagcg gtgaagtcgg agtacgcgaa
    16321 gttcatcaac tggatgcagg agcagtccgg ctcgcagtcc acgcggtatc tgttccggcc
    16381 gctctccgaa gtgaaggagg aaaaggtcct cggcggcacg tggctaccat ggatctggct
    16441 cggagatcag gacaggggtc ttgaggtgac cacctgggac gtgggtgctc aaacctacaa
    16501 ccgtattgac tccacccgcc gccaggatca gaacgaggtg ttcaccgttc ggcgcgccgg
    16561 cagcggagtg cgctgggaga actggctggt gaaccggacc tttgtttatg tcaggaaggg
    16621 gtggcagcgg ggcggggaat tcgcgctggc ggtcacacca tccaagaagt tcgacccgta
    16681 ctactccatg gtcaagggag ctcatctcgg accgcatcag cacgagcaaa cactaaagct
    16741 gcccgacgaa ggtcagatcc gaacccttgc acagaacgga ttcaacctcg tcgtcggcat
    16801 ggccaactgg cgctcaggcg agtatattcc gctgaacgac gccgatttgc gccgcaccat
    16861 cgagctttgc cacaagtacg gcatgaaaat catcccgtat atcacgctcg tcgacctctc
    16921 gcacgccacg gaggcgttcc gcgatcacgg cgaggagtgg gccatcgagc ccacgaccga
    16981 atactcgaag agcggttcgc ggatcttccg gcgagatccg cgcggtgaag cggcctatcg
    17041 gaacgacccg gagcaggaaa cgaccttgat gtgccccggc gcggccggct ggcgcgagca
    17101 ttggaagaag caggtcgatc gtttcctccg tgagtacgat tttgacggcc tgtacatcga
    17161 cttctggtac ggccggatgg tctgcgagaa cacgcggcac gggtgcggcg gccggtatcg
    17221 gaaacacacc gtcctcggct cccgagatat gctcacgtat gcctacaacc ggatcaagtc
    17281 aaaagaccct cgcgcgatcc tcaaggccaa cacgaacacg ctcgccacag cgttgatcac
    17341 cagcctggcg gaattacgtc tggttggaga gtccatcgac atgacgactc tcgactcgga
    17401 cagccggcag tggctgtaca gttcgtaccg cctgggtgag ccgacagaat ttctgtggga
    17461 ccgcactcgc tgggaccctg cacgaaagaa cggatttgcc gcgctcgtga acttcctgcc
    17521 gcaggtctac gatcggccgc cgttcgagcc ccgcaagacg tttgacgact tcgacgtctt
    17581 ccggtcgttt gaagcggaga aagggcggtg gtatctcggc atcgggggcc acggccgctt
    17641 gacggccacg ccttccggcg tttcggtgaa cctcgtcgaa cgggaggccg ccaggctcgc
    17701 gaccgtgatc aacccggctg aagctccggt caccgcctcc attccggtga aaccaggcac
    17761 actggcgtac gaaccgttgg cggaacgtct gctcgacacg gccggcggag ccgtcgctgt
    17821 agagattgct cccggcggtt accggcacat gattctcatg gaatcgccgg agagacccta
    17881 tcttctggca gcgctcgggg ctcggcggcc acttccacag cagtgggacg gacaggcgcg
    17941 ccgactccgg gtttcggcgg aaggcgtgga aggcgcgcgc atccgctatg ccgtctacag
    18001 cgcagagcgg ccgaaggaac ttgtaggcgc gcggggcgct cgtgttccct tcgagtggac
    18061 ggccgagacc cgtttggttc gcttcgaaac atcccactca tcggcggagg tcttcgagtt
    18121 gcggttctga cgccggctca cggcacgcag ggccgcgccg gagcggacag cgagatgacc
    18181 cagcaccctg gtttcgtgat gcggccggca tcgttgccgt tccagttcac cgccaccgga
    18241 gcccaatttt ggggcagggt catccgggct tcggcgaccc cgcggctcaa tacttggatc
    18301 tcgtccttgt tcgggacgta ttcgacctga atgcgggccg tgttgatttc ctcgcgtttg
    18361 ggcaggacaa tgcgggtctc gacccgctgc ctcttccccg cgcgctcgat catcagagcg
    18421 acaggttttt cctccttcat ttcttccagc attttcatgt aatgctgccc atcgcgaatc
    18481 tcgcggccgg caagcgccac gatccggtcc ccctgcgtca gcggcccctt gaaccctggc
    18541 ggaaggtact gaatcaagac accggggccg ggcgccgacg ggttgaatcc gaagcttccg
    18601 atcgacagaa atgctcgggt tcccggcggt acgcgcgtcg aacggagaac gtcgttgcgg
    18661 agcgacggat cgaatctcgt catctcaacc cagtagcagc gtccaaaacg gttgtcaccg
    18721 ctttcacaat cgaccttgcg gggaaattcc tcgcggcggt gtttggccag ccactcgagc
    18781 acatggtcga tggcaatccc ggcgctcgat tgtagttgga cgttatatcc ggcggttgtc
    18841 agcgtctggc ttagcgggag cgcggttgcg tggtccgaga ggcggacaga ccacaagatc
    18901 gggacgagac tcgtattcgc cccaaacagc cggttggagg ccattgccgc tcttggactc
    18961 ccctcgaccg cgaccgccgc cgtccataga tcgggaacac ggctggccag atagaacacc
    19021 tgcgatcccc cctctccgat acccgccagg tacacccgtc cggggtcaac ctgaaaccgt
    19081 accttggcgt cctcgacaat cgcctcggtg gcctcaactc cggcgtcgtt aatcgccggt
    19141 ccggtagctt tcgcccacgg tacgatcata tgccagcgcc gccgcgcagg ggccccttgc
    19201 caattggttg cgaaccgccg tatcgcggcc agatccgcgc ccgtgtcggg gtgcagaagc
    19261 accaggagcg gcgccgggcc ggcggcggaa gcgcttgccg cgcacacgta cgtcagctcc
    19321 ttcccggcaa acggaacccg gacttcgcga agttgctgcg cgccggcccc gagggcggcg
    19381 aaaagaagca gcgtggaaag acgcacgatg atctcctggt cagaaccgct tcaggcggtt
    19441 tctgatacgt ttggccagct tctcgagttc ctcggctttc ttcagcgacg agacggagag
    19501 gacatgtcgg tcgttcttct cgagttcaat cttcaaactc tcagccagtt cgacgagccg
    19561 gccggcgtcc gccagggtct tcgcgtattc ctccttcagg attgcctcgc gctgggattt
    19621 gccactcgga agccgcggct ccggctcctg tcccggcggc gctatctgac ccgcggcgag
    19681 aagcgccatg aacgcgagca gcgtcacggt tcgcggacaa cgagcacgta tcttcatttc
    19741 ggtatttcca tcatatcgtc ggtggaaacg aaggggttgt tatcatagct ttcttatgga
    19801 cgatgtgaac gttggcattg tcggcctcgg caacgttggc atgggtaccc tcggcatcct
    19861 cgccgggaat gccgaacaga ttgcgctgaa actcggtttc cgcctccgcg tgagggcggt
    19921 ttgcagccgg acggccggga ggaagacatt gccggcgggg cttggagacg tgttcatcac
    19981 cacggactgg cgtgaacttg tctctcatcc cgaggtcgat atcgtggccg aactcgtcgg
    20041 cggcaccggt gtggcggcgg agattatcga gggggcgata acgaaccgaa agtcggtggt
    20101 tacagccaac aaggaactga tggcgctctg tggcgcggag atttgggatc gggccatccg
    20161 agccgggatc aatcttgcga tggaggccag tgtcgccggc gggattccaa tccacgccgt
    20221 cctgcgggaa gggatctccg gcgaccggat cgtcgctctc tacggaattc tgaacggcac
    20281 cagcaactat atcctgacgg agatggagaa acggagcgcc tccttgccgg atgtcctcgc
    20341 ggaagcccag cgcctcggat tcgcggaggc cgacccgagc gccgacatcg acgggtggga
    20401 cgcacgctcg aagcttgcca tcctggcagc cctggcgttc ggcgagaaga tcacgccgtc
    20461 ggacattttc accgagggca tccgccgcat ctctcaggtc gacttcgagt atgcgcgcct
    20521 gctgcgccac accattcggc tcgtgtgcgg agcgcagcag acccacgatg gactcatcct
    20581 gtatgtccgc ccggcgctca ttcccctctc gaccatcctg gctggcgtcc agggcgcata
    20641 caacgccgtc tgggtcaaag ggtactacgg cgcggatact ttctattacg gccgtggcgc
    20701 cgggcccaat ccaaccggtg tcgccgtggt cagtgacctg atgcgggtgg cgagggaaat
    20761 ccgaaacggc agtccggagc gcgtctcgcc gtttgcgcac gagcgtctcg gcgagtacac
    20821 tcccattgcc gtcatacggc aaaagcgggc ttattttctc cgttttcggg tcgtcgaccg
    20881 gcctggaatc atcgctgcct tagccggtat tctcgcggaa aaaaagatca gcctggaggc
    20941 agtgctccag cttccctgtg ccggcaaaca ggacgtgccg tttgtcatta ctgtcgagcc
    21001 gacatccgag cagtccgtcc gcgaggcggt ggaagagatg tctggtctcg atttcctact
    21061 cgagccgccg ctggcgctgc cgatggagcc cggtctttag cgttcacgca gcgttttccg
    21121 gtccgatggc ggcggcctgc gtgctagact gtcggattgc ttgccgtcgc cattgatcgg
    21181 gccgccggct tgcctgactc gatgctacgt cttttgcggg aactcattcc agtcagcgtg
    21241 ctgacgcttc tggtatccga gttcgttctc tacggatcgt gtttcgtact ggcgagcttc
    21301 attgttctag aggtagatcc gaccgtattc ctactctatg acggtggctt ggggcggatc
    21361 gttgtcgccg tcctcagtat ccttcttggg ttgtatttcc acgatctgta ctcgcaattt
    21421 ctcgtaaaat cgcgggtttt gctccttcag caaagctgcc aggtgattgg aatcgcgttt
    21481 ctgatccagg ctctgctcag ctacggaagt gcagactgga tgctgccgcg ctggctgatg
    21541 atgctcggca gtggtctgac gctcgtggca atgctggcct ggcgcatcac ttacagcagc
    21601 gtcgtgttgc gggccatggg tgaagcccga gtcctgtttc ttggcgacaa tagtctcgtc
    21661 cgggaaatgg tcgcgcagat cgcggaacag cccgaactgg ggctgaaggt cgtggggtat
    21721 ctggatgaag gtctctcccc ggattgtgac tggcaggggg cgaccaggct cggcagtgtg
    21781 gaactcctca gggacgtggc tggccaagtg aagccggaca ggatcgtcgt tggcatgcgc
    21841 gagcgccgcc agcggcttcc ggtgtccgat ctgctcgacc tccgcctctc cggcgtccgg
    21901 gtcgaagagg ccgccacaat gtttgaggcc atctgtaagc ggattgccct aaaggacctt
    21961 cgtccaggac agttcatctt ctcagccgac ctcgggccgc gcccaggcag cattctgttt
    22021 cggtccgctt attctggcct tgtggctctg accggcacct tgctcaccct gcccttgatg
    22081 gcggcggccg ccatcgcgat caagttgacc tcggcgggcc ctgttctttt tcgccagacc
    22141 cgcgtcggcc ttcatgggcg gccgtttacc ctctacaagt tccgttcgat gtatcaggat
    22201 gcggaagtct ccaccggcgc tgtgtgggcc acccgtgacg atccgcgcgt caccccgctc
    22261 ggccgctggt tgcggcggct ccgcttggac gaactgcctc aatttttcaa cgtcctgaga
    22321 ggggagatgg tgatcgtcgg cccgaggccg gaacggccgg agttcgtcag caccctcacc
    22381 gatgaggttc cgtactacgg gcagcggaat tgggtcaagc ccggcattac tggttgggcg
    22441 cagatcaacc acaagtacgg ggacaccgtc gaggactccg tcaagaagct ggaatacgac
    22501 ctctactaca tcaagaacat gtcccttgct ctcgacctct tcatcatctt tcacaccgtc
    22561 aagaccatgc tgctctcacg cggcgcccag tgagaactgc cgttgtgagg agccgtttcg
    22621 ccgcgttgac cgccgggtat cgctttccgt acaataaagt tgcagacgag cgggagtaac
    22681 tcagtggtag agtcacagcc ttccaagctg ttggtcgcgg gttcgattcc cgtctcccgc
    22741 tccaagacat tctgctagaa taggaaggtt tgccctgctg gtgtagctca gttggtagag
    22801 catctgactt gtaatcagaa ggtcgtgggt tcaaatccca ccgccagctc caggccgtac
    22861 ccccgccgcg gatgtgctct gcgggagaca agccgatacg atggacgggt ggccgagtgg
    22921 ttaatggcag cagactgtaa atctgccgcc ccttgcgggc tacggaggtt cgaatcctcc
    22981 cccgtccacc aggttgatcg ggcagtacgg cgtggccggg ctgagccgtt gtagctcagt
    23041 tggtagagtg tccggcgggt cggaaggtcg ccggggcgcc cggcgggcag cggcaaagag
    23101 ttcgggccga tgtagctcag ttggtagagc gcgtccttgg taaggacgag gtcaccggtt
    23161 caatcccggt catcggctcc agggattgaa ttattttttg tacgtgcttc agagcgtccc
    23221 gacaggtcgt cactacatcg ggataacagc ggacgttgac aggcgtctgg aggagcacaa
    23281 gagcaggaat ggccgatgga ctagttcctt tgcgccatgg caactgctcg ggacggagcg
    23341 gaggcgattg tttgagcggc ttgacggagc aggcggccga gccggtttga acgggtagag
    23401 cgtccgtggt cggaaggtca ccggttcaat cccggtcatc ggctccagag agtcttctcc
    23461 acggactcat cgcagagagg aagcgaacgc gcagggcccc ggctgagtat tagagttcaa
    23521 gaccaagggt gacagtatgg cgaaagaaaa attcgatcgc agcaagccgc acgtaaatat
    23581 cgggacgatc gggcacatcg atcacgggaa gacgacgctg acggcggcga tcacgaagtg
    23641 tctggcgaag aacaacccga aggtgaagtt ccggag
//
LOCUS       DATIFQ010000371         5704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1082092, whole genome shotgun sequence.
ACCESSION   DATIFQ010000371 DATIFQ010000000
VERSION     DATIFQ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082092"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VLE22_18000"
     CDS             complement(<1..684)
                     /locus_tag="VLE22_18000"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HSB16348.1"
                     /translation="MKNVMLLALSFAIGSIAFAQSTALPIKVSENGRYFVDQNGNPVF
                     WLGTTQWELFHGFTQEDAKVTIEESRKNGFTFVQVKLMGGGDGTKPNVYGQKAWLNDD
                     PLMPNEAYFRNVDAVIQMARDNDLVILPSVYHQSYRKHITMEKARRWAKWLAQRYKNA
                     PNIVWTMTPEARQDFVPVIRELAAGLREGDGGRHIISLKPDPSPYSSSFLHSESWLDF
                     NSMQTWNAVK"
     gene            complement(804..2324)
                     /locus_tag="VLE22_18005"
     CDS             complement(804..2324)
                     /locus_tag="VLE22_18005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HSB16349.1"
                     /translation="MPLFRTRSAAVYGIDAHPIDVEVDMWASGNARDFITVGMPDTAV
                     RESRERIKSALMNSGFGYPNKSVTINLAPANVRKEGAGFDLPMAVGILGAMGVARKTD
                     DYLLAGELSLDGAIRSVRGALPIAVCALRQGIANLVIPFENASEAAVVEGVRVFGIRN
                     LAEVVALLERPAEFAPAHVSPPSPGVIEEPPPDFNEVRGQATAKRALEVAAAGGHNVL
                     MIGPPGSGKTMLAKRLAGILPPLTFEEALETTKIHSVAGILQKGTGLLRSRPFRAPHH
                     TVSDAGLIGGGAGTPRPGEVSLANHGLLFLDELPEFARNVLELLRQPLEDGSVTIARA
                     NMTLSFPANFMLVAAMNPCPCGFHGDATRECRCTGGIIQRYLGKISGPLLDRIDLHVE
                     VPAVAYKEMRSQAAGTTSAEMRERVQCARRIQRARGFYNSRIPARMLRKLCELDDAGE
                     RTLEMAVRRLGLSARAHDRVLKMARTIADLSESQSVTAKHVAEAVQYRSLDRNYWS"
     gene            2825..3604
                     /locus_tag="VLE22_18010"
     CDS             2825..3604
                     /locus_tag="VLE22_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16350.1"
                     /translation="MPPRKAPIQKQPTLAPDRASRALAQQIDALQKLKGCEYQEADAE
                     ETEWEHLTQGIIEAAFGDPSSALSKFHMAKAAGQHNIMGIPPQQRQINFELRVKEQEA
                     LLRSLISTLRLQIPEAEIKGVYEPGDEYSFYRDLSSLIQATTQDILIVDAYLDEQLFN
                     LYVSKVPDRATVRILSGKIGPNVETVARMYANNRPLEMRSTANVHDRAIFLDQRGWVI
                     GQSIKDAARKKPTYLIELTEPSLTAARDAHNRIWAAAAVVI"
     gene            complement(3597..4022)
                     /locus_tag="VLE22_18015"
     CDS             complement(3597..4022)
                     /locus_tag="VLE22_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16351.1"
                     /translation="MHWRSPIVGVASVIILATCTRQPTKPVEDLGGGQDVSRFALTSL
                     KGTRNGDRLDVQAVYSHTFENITVDLHFRVTPPTRLESGTWNGLTKEGAVRERSSTFL
                     GGQSGPPSIGGRFDLMAPNGQPLYRVTIPLQPLKHRLSR"
     gene            4898..5101
                     /locus_tag="VLE22_18020"
     CDS             4898..5101
                     /locus_tag="VLE22_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16352.1"
                     /translation="MAPLQDRVRAMVRAITEGYALMGRERIEHTLRLQDELGEFFWRF
                     APTWIEDRYDAAMSSVPARLAHA"
     gene            5448..>5704
                     /locus_tag="VLE22_18025"
     CDS             5448..>5704
                     /locus_tag="VLE22_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HSB16353.1"
                     /translation="MAFAIALLQIEPFGMDQGRNLEKGLKCCRDAKSMGADLAVFPEL
                     WNIGFARCPIDATGRQSWIASAIDRGSAFFRCFADLAKELG"
BASE COUNT         1098 a         1696 c         1774 g         1136 t
ORIGIN      
        1 cttgacggcg ttccacgtct gcatcgagtt gaaatccagc cagctctcgg agtggaggaa
       61 gctggaggag tagggcgaag ggtcgggttt caggctgatg atgtggcggc ctccgtcccc
      121 ctcgcggaga cccgcggcca attcccgaat gaccggcaca aaatcctgcc tggcttcggg
      181 tgtcatggtc cagacgatgt tgggtgcgtt cttgtatcgc tgggccagcc acttggccca
      241 acgtcgggcc ttctccattg tgatgtgttt ccgataggac tgatggtaga cgctcggaag
      301 aatgaccagg tcgttgtctc gcgccatctg gatcacggca tccacattcc tgaaatacgc
      361 ctcgttcggc atgagcggat catcgttgag ccaggccttc tgcccgtaca cattgggctt
      421 tgtgccgtca ccgccgccca tcagcttgac ttggacaaag gtgaaaccgt tcttcctgga
      481 ctcctcgata gtgactttgg cgtcctcctg ggtgaacccg tggaagagtt cccattgtgt
      541 ggtgcctaac cagaacactg gattgccgtt ctggtctaca aagtacctgc cgttctcgct
      601 gactttgatg ggcagcgccg ttgattgtgc gaacgcgatc gagccgatgg cgaacgacaa
      661 ggccagcagc atgacgtttt tcacgattgg tctcatctga gattgccgtt ctgtcttcct
      721 gggcacgcgg cgattatatc tcaaacacct ttggggaccg ttcttttact ggttctgttc
      781 cgagcggatc tgagggaccc acctcagctc caataattcc ggtccaaact gcggtattgg
      841 acggcttcgg cgacgtgctt ggcggtgacg gattgggatt cggagaggtc ggcgatggtg
      901 cgggccatct tcaaaacgcg gtcgtgggca cgggcggaga ggccgagccg gcggacggcc
      961 atttcgaggg tacgctcacc agcgtcgtcg agctcgcaga gtttgcggag cattcgcgcg
     1021 ggaatgcggg agttgtagaa gccgcgcgcc cgctgaatcc gccgggcgca ctggacgcgc
     1081 tcgcgcatct cggccgaggt cgtgccggct gcctggctgc gcatctcctt gtacgcgaca
     1141 gccggcacct cgacgtgcag gtcgatgcgg tcgagcagcg gcccactgat tttgccgagg
     1201 tagcgctgga tgatgccgcc agtgcagcgg cactcgcgcg tggcgtcgcc gtggaaaccg
     1261 cacggacacg gattcatcgc cgccacgagc atgaagttgg cggggaagga gagcgtcatg
     1321 ttggcgcggg cgatggtcac cgagccgtct tcgagcggct ggcggagcag ttcgagaacg
     1381 tttcgcgcga actcaggcag ctcgtcaagg aaaagcagac catggttggc caggctcact
     1441 tcgccggggc ggggggtgcc cgcgccgccg ccgatcaacc ctgcatccga aaccgtatgg
     1501 tgcggcgcgc ggaacggccg ggagcgcaac aggcccgtgc ccttttgaag gatgcctgcc
     1561 acgctatgga tcttggtggt ttcgagcgct tcctcgaagg tcagcggcgg caggattccg
     1621 gcgagccgct tggcgagcat cgttttgccc gagcccggcg gcccgatcat gagcacgttg
     1681 tgaccgcccg ccgctgccac ctcgagagcc cgcttggcgg tcgcctggcc gcgcacctcg
     1741 ttgaaatcgg gcgggggttc ctcgatcacg ccgggcgaag gcggcgaaac atgcgccggg
     1801 gcgaactccg ccggccgctc cagcaacgcg accacttcag cgaggttccg gatgccaaag
     1861 acccggacac cctccaccac ggcggcttcg gaggcgttct cgaacggtat cacaaggttg
     1921 gcaatcccct gtcggagggc gcagacggcg atcggcagcg cgccgcggac cgaacggatg
     1981 gcgccgtcta gcgacaactc acccgccagc agatagtcgt ccgtcttgcg cgcgacgccc
     2041 atggccccga ggatgccgac ggccatcggc aggtcgaatc cggcgccctc cttgcggacg
     2101 ttggccggcg ccaggttgat ggttaccgac ttgttcgggt agccgaagcc cgagttcatc
     2161 aaggcggact tgatgcgctc gcggctttcc cggacggccg tatcgggcat gccaacggtg
     2221 atgaagtcgc gggcattgcc ggacgcccac atgtccacct ccacgtcgat ggggtgagcg
     2281 tcgatgccgt agacggcggc gctgcgtgtc cggaaaagcg gcaataaaag aagtgcgccg
     2341 gagagcgcaa tcgtctcact ctgactgcag tggggtcgac accgttggac ggaccgtcgt
     2401 agttttcaga atcgtatccg attaaccctg gtatcctccc agatttcgac gtccgactgc
     2461 tgcgtcacgt ctcgcatatg ggcactcaaa gtgtttcggt ttgtcggtaa gaatcgtcac
     2521 ttgttggttc cgcgtggact ttgctccagc tcgtgcggca tcgaaccagc cttgggctga
     2581 cgcaagtgga ggcagcgaag cgccttggcg ttgatcccag tacgttggcg aagtgggagc
     2641 gcggggagag ggagcctcaa ggcatcctgc gagtccgcgt ggagcgattc ctccaggacg
     2701 gggatgtatc ggacgcgcga cgagctgggt agtccaaaga gagagacgtc ccgattccac
     2761 gcgccctggc cccatcgttg cttccgtgtg ataacgtcct ttatgacact cgagagggag
     2821 gatcatgccg ccacgtaaag cacccattca gaaacaacca accctcgctc ccgaccgggc
     2881 ctcgagagcc ctcgcgcagc agatcgacgc acttcagaaa ctgaagggct gtgagtacca
     2941 ggaagctgac gccgaggaga cagagtggga acaccttacg caaggcatca tcgaagctgc
     3001 cttcggtgac ccgagttctg cgctgagcaa gtttcacatg gccaaggccg cgggtcagca
     3061 caacatcatg ggcattcccc cgcagcagcg ccagatcaat ttcgagttgc gggtgaaaga
     3121 gcaggaagcg cttctgcgga gtttgattag cacgctccgg ctacagattc cagaggcaga
     3181 aatcaagggc gtttatgagc ctggtgacga gtattccttt tatcgcgatc tgagctctct
     3241 cattcaggcg acgacacagg acatcctcat tgttgatgct tacctcgatg agcaattgtt
     3301 caacctctat gtcagcaagg ttcccgacag ggcgaccgtc cgcatcctct ccggcaaaat
     3361 aggccccaac gttgaaaccg ttgcaaggat gtacgccaac aaccgccccc tcgaaatgcg
     3421 gtcaaccgca aacgtgcatg accgggccat cttcttggat cagcgcggat gggtcatcgg
     3481 gcagtcgata aaagacgccg cgcgaaagaa accaacctac ctgattgaac tcactgaacc
     3541 gtctctgacg gccgccagag atgcgcacaa ccgaatctgg gcagctgcgg ccgtcgtcat
     3601 ctagagagcc gatgtttcaa cggctgtagc ggaatcgtga cgcgatacag tggttggccg
     3661 ttcggcgcca tcaggtcgaa acgtccgccg atgctgggtg gtccggattg gccgccgaga
     3721 aaagtcgacg agcgttcccg cacggcaccc tcctttgtca ggccgttcca agtccctgat
     3781 tcgaggcgcg tcggcggggt cacccggaaa tggagatcaa ccgttatgtt ctcgaatgtg
     3841 tggctgtaga cggcctgcac gtcgaggcga tcgccgttgc gagttccttt caacgacgtg
     3901 agcgcaaatc gcgaaacgtc ctgtcctccg ccaagatcct cgacaggttt ggtaggctgc
     3961 ctggtgcagg tggccaggat tatgacgctc gccactccga cgatcggcga tcgccagtgc
     4021 atcccttaag tctacgtctc cgtcagtgcc tgcagcgaag acctggctga gcaattcgtc
     4081 cggcatcgga ctacgccggg cctgtcacag aaggaggcgg cgctgaagct cggcgttgac
     4141 cccagcacgt tggcaagatg cgaaaggggt gaaagcgtac cggcggacgt gcttctggcg
     4201 cgggttcgga gcttccgtga ggttacagag aaggccccgt tggtcttgca gcactgctgg
     4261 ataggcccga caaacccacc gggccgtgtg gctgcttgag cagatgtcca gcaccctaaa
     4321 cgagtcccgc ttggcgacct gtccgatatc tgttgtttcg gggcgcgttc gcgtccctgt
     4381 taactagggc cggcggaaat tagaagattt gcagctttgt tgggcaaata ccgatgagga
     4441 cgtgtcacat taggctttcc gggtcgtgga ccatctcggc ttggtcggtc aatggagtat
     4501 gagagcagca gcggccagca gttcaccgat agcgagcgtc agcaggcaga tcgaaacgga
     4561 tcggtacttg ggcaccgcca gccgatccat cgcccttttt cagtgccgtc tgccatgttt
     4621 aggcggcccc acttcaatct ttctctgtac gaactcgatt tcctcctcgc cgagaactgg
     4681 gacctccggc accggccgct gtatcacgtc ctggtcagcg ccaccagcct tccctgggag
     4741 gcacggacct ttgcgacgca atgggacatc atgaccatcg agccgacacg cctccccttc
     4801 ttcgtccttg atgcgcgggc gcgtcgccga ttcacagcac tcccggaagc cgttcgggcc
     4861 gaggcagaca ccatcctggt gagatgttcc tgtggtcgtg gcgccactac aggaccgcgt
     4921 gcgcgcaatg gtgcgcgcca tcactgaggg ctacgcgctt atgggcaggg aacgcataga
     4981 gcacacgctc cggctccaag atgagctcgg ggagttcttc tggcgctttg ccccaacctg
     5041 gatcgaagac cggtacgacg cggcgatgag cagcgtgccc gcacgcctgg cacacgcatg
     5101 agcatcacag acgccgagtc gtcgaccttt cttgcgtgtt gtggcaccgt cagaaaccgg
     5161 cggtaatcac ttatatgagc attaatcttg acaagatgcc cctcgccccg acacggccgt
     5221 tgccgcgcga caagagatta gcgtggctcc ccaccttgga cacgtttcga actcttgcgg
     5281 cgtgaaaggc ggcctgctcg aaccgaatca actcgttgat gggattcgga gtagtgtctg
     5341 tgaaggcagt tgcatccagg ctgggtgccc atgtcggatg tgtctcgaac cggcatcgct
     5401 acaactgcgc cgccagagca agccaaatct gttaacttac gaattcaatg gccttcgcga
     5461 tagcactgct tcagatcgag ccgttcggca tggaccaagg ccgcaatctc gaaaagggcc
     5521 tgaagtgctg ccgcgacgcg aaatcgatgg gcgccgatct cgccgtcttt ccggaactct
     5581 ggaacattgg attcgcacga tgcccgatcg atgcgaccgg acggcagtca tggattgcgt
     5641 ccgcaatcga tcgcggaagc gctttttttc gatgtttcgc cgatctggcg aaagagcttg
     5701 ggat
//
LOCUS       DATIFQ010000372        61559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2806599, whole genome shotgun sequence.
ACCESSION   DATIFQ010000372 DATIFQ010000000
VERSION     DATIFQ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 61559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61559
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806599"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VLE22_18030"
     CDS             <1..549
                     /locus_tag="VLE22_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16354.1"
                     /translation="PGESRCNGKWHPVCRYMSDVAGVPALEVVYLSMSESVRELIKQY
                     QAFCEVAPYYVVFEERSSNGAITKQRIQAGLDIDVYGVKSGLGPGPLSDYALGCAALR
                     EVAQTVTSQTGDSCSIEVIPFASTVILDTRRHFQPQGMIRIRITRGRGIGQPAGEPEE
                     KVLKAILTQLNDLGISSGNSRT"
     gene            complement(2342..3895)
                     /locus_tag="VLE22_18035"
     CDS             complement(2342..3895)
                     /locus_tag="VLE22_18035"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HSB16355.1"
                     /translation="MLFSAVVETSRRVSETSRRLEKIDLLAGLLHRLDPEEVEIVVAF
                     LSGYTRQGRVRIGYATLRAAAADPNEAAALEIHVLDRALTELAAVQGSGSERRRRDLL
                     HSLLSRSTAPEQQFLTGLLLGEIRQGALEGIMLEALAKASGISAPRVRRAAMLAGDIA
                     RVARSALESGAAGLDSYDIQLFRPIQPMLAQPHEDVAGALADLGEAALEYKMDGARIQ
                     VHRSGDDVQIFSRALNNVTAAVPEIVEVVRALPARDIILDGEVLSLTPEGRPRPFQVT
                     ARRFNRKLGLDRMRAELPLSPFWFDLLYLDGQSLLDEPQSRRFSSLAGLSPAEALIPH
                     TRTADSNRAAEFLRQALEQGHEGVMAKAIDSPYAAGARGQSWLKIKRPHTLDLVILAA
                     EWGSGRRRGWLSNLHLGARDTDKGGFAMLGKTFKGLTDDMLAWQTEQFLQAEIGRDEY
                     TVYIAPKFVAEIAFNDIQISPHYQSGLALRFARVKRYRVDKTPDAADTLETVRALAGL
                     GGATASSSS"
     gene            4305..4886
                     /locus_tag="VLE22_18040"
     CDS             4305..4886
                     /locus_tag="VLE22_18040"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HSB16356.1"
                     /translation="MAAKRILMLVGDYVEDYEVMVPFQALRMVGHQVDAVCPGKQAGD
                     VVRTAIHDFEGDQTYSEKRGHNFALNAAFDAIRPEDYDALVIPGGRAPEYLRLNPSVL
                     EVVRHFSRADKPIAAVCHGAQLLAAAGVLEQKCVSCYPAVGPEVTRAGGRYMDVAMDQ
                     AHVDGKLVTAPAWPAHPVWLEAFLKVLGTRIEL"
     gene            complement(5243..6406)
                     /locus_tag="VLE22_18045"
     CDS             complement(5243..6406)
                     /locus_tag="VLE22_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16357.1"
                     /translation="MNTLWTGFRLSPPFERCLYGMAAGGFLALAFGLLLAPQRALGSL
                     LMAGYGLVCLGLAGILFVAIHYASGAAWGTAFRRVPEAMCAALPYGAAMLAVVFLYGS
                     PLYPWIRDRGEMAGFRAVWLSFPFFLARAILYVGLWFLLTRAILKTSRLQDDTRDPGA
                     TPRNARLSVIFLLVFGVTFWLASFDWIMSLEPHWSSTIFGIYNFAGMFSSGLAVIILL
                     VVWMRRAGPLRDFVTDEHLHDLGKLLFAFSTFWMYIWFSQYMLIWYADIPEETVHYIK
                     RQHQFWMPLFIANMVLNWVVPFVVLLPTRTKRTGSLLAKAAAVVLVGRWLDLYIMIQP
                     ATGGSNPRFGLWEAAALVGVIAIFILVFSRAMNSAPPVPLGDPHLAESVNYHN"
     gene            complement(6403..7044)
                     /locus_tag="VLE22_18050"
     CDS             complement(6403..7044)
                     /locus_tag="VLE22_18050"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB16358.1"
                     /translation="MKLNLLLAVALVLCVAFGLFVRWQPSRPNLEVLPEMVRTASYQS
                     YSANPNFPDGKTLQEPPPGTIPRGFHAVDYQAGEADAIRAGQELSNPYPAGNPAALTR
                     GAFVFRTWCLPCHGATGRGDGPVALRGYPAPPPLNSEKTLGLNDGRMFHILTYGQKNM
                     PSYASQVSEDDRWKVILYVRSLQRSAQTSQTPQTPVAQPVPAPSGPNGREEKK"
     gene            complement(7041..7571)
                     /locus_tag="VLE22_18055"
     CDS             complement(7041..7571)
                     /locus_tag="VLE22_18055"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HSB16359.1"
                     /translation="MSGRVMVGIFQDESDILSVTREVRKQGYTIRDIYAPYAVHGLDK
                     AMGLQPSRLPWVCFLLGLIGAAAKVAFEYWTTASDWPVNVGGKPWNSLPAFVPITFEV
                     MVLFAGMSTVFAFFLVSRLYPGRRAASVIPGVTNDRFALVLQETDAAFEPVLVRRLLE
                     RHGAVEVIERAEESLP"
     gene            complement(7568..8941)
                     /gene="nrfD"
                     /locus_tag="VLE22_18060"
     CDS             complement(7568..8941)
                     /gene="nrfD"
                     /locus_tag="VLE22_18060"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HSB16360.1"
                     /translation="MAEVQAVPRPPLIQGDRSPGQITGDICAAMLRRPGALWWAGLLV
                     SLSLLAGGVAAVSYQVATGIGTWGLNNTVGWAFDITNFVFWIGIGHAGTLISAILFLF
                     RQRWRTSVNRSAEAMTLFAVMCAGIFPLIHMGRPWMAFWAFPYPNFRGPLWINFRSPL
                     AWDAFAISTYFIISLTFWYLGLIPDLATIRDRARAGIRRRLFDMFSLGWDGSYRTWQR
                     YETVYLLLAGLATPLVVSVHTIVSWDFATAVIPGWHATIFPPYFVAGAIFSGMAMVLT
                     LMIVARKVMNLENYITLRHIDVMCKLVILTSSIVALAYGTEFFTAQYSGNPYEQFVFL
                     NRAAGPFAWAYWTMVTCNVIVPQVLWFRRVRMNVVLVFAVSLLINVGMWFERFVIIVT
                     SLHRDFLPSNWASYRPTVFEVATLLGTFGLFFTCFLIFCRVLPVIAMAEVKGAAAHER
                     SKGGNHQ"
     gene            complement(8934..11972)
                     /locus_tag="VLE22_18065"
     CDS             complement(8934..11972)
                     /locus_tag="VLE22_18065"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-cluster-containing hydrogenase"
                     /protein_id="HSB16361.1"
                     /translation="MSADNKTYWKSLEEYEQGGPSGERAAVEFVDTPLRDLSEPGRRD
                     FLRAAGFAFAGAALTGCGRAPVERVIPMLRQPEGITPGRPYYYAAVCGGCSASCGVLS
                     KCRDGRPIKLEGNPEHPVSKGGLCAVGQASLLGLYDSLRLQQAMARGRSAPWREVDAE
                     ISRALDSFRKQRRAVRVLTQTITGPALSSQISTFLASFQDARHVVYDVLSASPILDAH
                     LRTHGARVLPRYLFTKAAVIASFDADFLGTWISPVEFTAAYSAARDVDAQPARSSWHI
                     QFESRMSLTGTKADRRIKLAPAEIPAALEHLAVRIARKAGSAVLPGAGSRSPAPEDVM
                     DDLATRLWNARGRSLVVCGLASTDAQVLCNLVNHLLGNYGSTLDVDAPSNQKQGNDRE
                     LMALVDEIKAGGVAALFIHGLNPVADLPGGEELARSLARVPLLVSLAPHLDETSSLAR
                     YLCPARDPLETWGDSEPVAGVAAISQPAIRPLGQARPAIESFARWSGVDAPAYELVKG
                     FWKERVYPRQERQQSFDVFWKKSVEVGFALVKPQSPTLKPFDLSVVKPIDVVPPSGNG
                     LALVLYANVAMPDGRHAYNPWLQELPDPVTKAVWDNYASLAPATAERLSLKNGDVVRL
                     EASGTAIELPVLIQPGQHEQVVAVALGYGRSVSARFAGAGPKWLERRPTLGPNGKVGV
                     NAAPLIGWENGAVQYWPGDVKVTSAGKRQLLAVTQDHHSLTVPKHLDPGGGPRPIVQE
                     TTLGALHSGEAKHEGAHTPSETDLWPDDHPFTGHRWAMAIDLNACTGCSACVIACQVE
                     NNVPVTGKDEVLRNREMHWIRIDRYYSGPPDDVEVAHQPMMCQHCEHAPCETVCPVLA
                     TVHSAEGLSQQIYNRCVGTRYCANNCPFKTRRFNWFEYSREDRLANLALNPDVTVRSR
                     GVMEKCSFCVQRIQDARAEAKRQNRALRDGEIQTACQQSCSAKAILFGDLNDPKSKVT
                     ERMKSKRTYRVLEELNVRPSVGYLKIVRHHEPGKGEGRHG"
     gene            complement(11985..12638)
                     /locus_tag="VLE22_18070"
     CDS             complement(11985..12638)
                     /locus_tag="VLE22_18070"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSB16362.1"
                     /translation="MNSRLLTIVLATGLFFSALYLLTVARPVHLPGNQQDYEPVQPVA
                     YSHRLHAGELQVQCLYCHFNAEKSRHAGVPPAAVCMNCHTMVTASFGAVRSEEELATK
                     ENRKPRTIVSPELRKIYDALALDANRKPDPARTPQPIAWTRVYKLPDFVYFDHRAHVN
                     AGVACQTCHGPVETMERLRQVPDLSMGWCVNCHREATRNGVGGKKLFASIDCSTCHY"
     gene            complement(12654..13985)
                     /locus_tag="VLE22_18075"
     CDS             complement(12654..13985)
                     /locus_tag="VLE22_18075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB16363.1"
                     /translation="MRTLKGSPAQGLSGATLGFFIGFAAVALFGPTAKRFQEAMNLSP
                     IAVGFLVAMPALSGSLLRIPFSAWVDINGGRKPFLTLLGLGVAGMTGLTAVVSLYYPQ
                     QMTPGMYPVLLILALLCGCGIATFSVGIGQVAYWFPRQRQGVALAIFGGVGNLAPGLF
                     SFLIPVALTTLGLAGSYMVWLALLATGLVLYFIVGRNSAYFQLRELGSTPEEARRLAS
                     EYGQELFPAGNLKDSLKVSARTWKTWALVSIYFTTFGGFVALTAWLPTYWTGFFGLSV
                     VAAGFLTALFSILTSLIRVIGGVLSDRLREGGENTTILALLITIIGALVVVNAAQYEL
                     AIPGIVLLAFGMGVSNAGVFKIVPQAVPQAVGGASGWVGGLGAFGGFAIPPVMAFAVA
                     DLGKRGYAIGFIVFVFLALFALSMAWVLKYMPESAPHRSGAVPLAAAANRK"
     gene            complement(14003..14698)
                     /gene="narI"
                     /locus_tag="VLE22_18080"
     CDS             complement(14003..14698)
                     /gene="narI"
                     /locus_tag="VLE22_18080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HSB16364.1"
                     /translation="MNTTTHYLDQLLFNVLPYIAFFTFFLATIRRYRSRTFTYSSLSS
                     QFLENRQHFWALVPLHYGILVVLAGHIVAFLVPRGILLWNSHPLRLYILEAAALIFGI
                     LTILGLSAAIVRRFTVSKLKIVTSPVDWLLFALLVLQAITGVAVALFYPWGSSWFSIS
                     ATPYLWSLVRLNPDIGYITPMPWLVKLHIVNAYLLILLFPFTRLVHVLVIPNPYLWRK
                     PQVVRWYRGPAGI"
     gene            complement(14698..15237)
                     /locus_tag="VLE22_18085"
     CDS             complement(14698..15237)
                     /locus_tag="VLE22_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16365.1"
                     /translation="MTSVAPATGLIAELLEYPAAGYQQRLAEVMETLPRTDERVANLL
                     GKFATAVSARSLQQMEELYVQTFDLNPDACLEIGWHLFGEDYARGEFLVKLRQEMRRH
                     KVPDRDELPDSLLSVLPLLARMPEEEAAHFRARFLLPALEKLRRAVPESNPFADLLSA
                     LILLLAGDGTECGPAKGDS"
     gene            complement(15237..16727)
                     /gene="narH"
                     /locus_tag="VLE22_18090"
     CDS             complement(15237..16727)
                     /gene="narH"
                     /locus_tag="VLE22_18090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HSB16366.1"
                     /translation="MNVRSQVSMVFHLDKCIGCHTCSIACKNIWTDRKGAEYMWWNNV
                     ETKPGTGFPTAWEDQEKYRGGWECVDGKLRLKSTSKSKTVVNIFHHPNMPTMDDYYEP
                     WTYDYQHLFNAPEGPDQPVAVPISAVTGELINVKAGPNWDDDLGGSPVYAEHDSNLAG
                     LTEQQKEQLFAVERLVFFYFPRICNHCLNPSCVASCPSGALYKRGEDGIVLLDQNRCR
                     AWRNCISACPYKKTFFNWSTGKSEKCILCYPRLETGQAPACFHSCVGRIRYLGVLLYD
                     ASRIEEVATRPPDELVEAQRSLVLDPNDPEVIAAARASGIHDSVIESAQKSPVYQFVK
                     VWKIALPPHIEFRTLPMLFYVPPMAPVMSSREDGAVKNVSDDLFHDIDDARVPMKFLA
                     NLFGAGHEGMVRYALRKQKAVRWYRRAMTVGDVEDAVSERMLREADCSKEEAEAIYRL
                     TSLCTFEDRFVIPPMHREEAIEMMADPREHQQSAGFGFLTGPTRGL"
     gene            complement(16741..20385)
                     /locus_tag="VLE22_18095"
     CDS             complement(16741..20385)
                     /locus_tag="VLE22_18095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HSB16367.1"
                     /translation="MTTHGPLTWIKDETDPKLRSWEQFYRNRWQHDKIIRSTHGVNCT
                     GGCTWQIYVKDGIVTWEMQGLDYPSLESGLPPYEPRGCQRGISFSWYLYSPLRVKHPY
                     VRGALLDLWRSARAEHADPVEAWRSLVNDPEKRGRWQKARGKGGFRRLDWDTAVELIS
                     ASCIHTIQKYGPDRIAGFSPIPAMSMISYASGARMLQLMGGVSLSFYDWYCDLPPASP
                     EVWGEQTDVQESADWYNAKLLAVTGSNLNMTRTPDCHFAAEARHNGSKMWVFAPDFNQ
                     VAKYADEYIPIHAGQDGAWWMAAGHVILKECHHEQKVPYFHDYVKKYTDCTYLVELVD
                     REGVFEAGQLLRANRLAEYAGVENGDWQFLMWDAAESKPKMPMGSSGYRWAKEKGKWN
                     LALKDGLTGSGIDPVLTFLEQHDQVVDVRFDDFGAGTSLRRGVPAKRLETAAGKSVTV
                     TTVYDLMMAQYGVPRGLSGEYPAGYDDENAPYTPAWQEKYTGMSRDTLIRFAREWGET
                     ARLTGGKCTVIIGAGVNHWYHNNLMYRSAINALMLCGCVGVNGGGLAHYVGQEKLAPG
                     ESWSAIAFGKDWYPASRLQNAPTWHYMHTDQWRYERDYADYHTMPQNPLMRGITGTHT
                     ADVNVQAVRNGWLPFYPQFDRSPLEVVRDAQAAGAKTDDDVVRYAVDELKNRKLRFSA
                     EEPDAPENWPRVWFIWRGNALMASAKGHEYFLRHYLGTHDNAIAEEMAEGFVHDVEFH
                     KQAPQGKMDLVVDLNFRMDTSALYSDLVLPAATWYEKADLNSTDMHSFIHPLSAAVPP
                     CWEAKSDWQIFRTISKKFSELAAKHFPEPVADLVATPLAHDTPAEIAQPAIRDWMRGE
                     VEAIPGKTMPGLRVVRRDYKNLYNQFISFGPMVRANGLGAHGTHYKVDDLYDEALLTL
                     PRVDWDGKSYPSLYEDESVCNMILRFASVTNGELAYRSYRGMEERTGVPLVHLAEKNR
                     AVRISYKELQSRIQRLLNSPMWSGLVENGRAYSPFTYNVEALVPWRTLTGRQHIYLDH
                     PGYIQFGEHLPTFKPKPSAKDYSDLRFTKEAGKTLMLNYLTPHGKWHIHSTYGDNQRM
                     TTLSRGVEPFWINDHDASDLGIVDNDWVEVHNDHGVVVTRAAVSARIPRGICIQYHSP
                     ERTYSVPKSPLRNYRRAGGHNSLTRVRLKPNLMVGGYGQFTYHFNYWGPTGCNRDTHI
                     LVRRLPDLKW"
     gene            complement(20382..21257)
                     /locus_tag="VLE22_18100"
     CDS             complement(20382..21257)
                     /locus_tag="VLE22_18100"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSB16368.1"
                     /translation="MNSGNNWSPGRRLGTPASLLWLAACVVAAPLTAQEAADFFRENC
                     TSCHTIGGGRLTGPDLKGVLARKDREWLARFVQNPQALMDSGDPYALKLRQEARDVVM
                     PTINGMNASRAEALLALIEAESKLKKSQFMGLQITDRPFTPSEIDRGRLMFSGVNRLA
                     NGGPPCISCHTARDVGGLSGGRLGPDLTRVFERLQGRKNLAAWLTAPATPTMVSVFKR
                     RALRPEEILPLIAFLEDEARKGGEDASPAPLTFFLIGLGGAALALVLFEQAWKERFRS
                     VRKALVLASTHRGEE"
     gene            complement(21254..21721)
                     /locus_tag="VLE22_18105"
     CDS             complement(21254..21721)
                     /locus_tag="VLE22_18105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSB16369.1"
                     /translation="MLSTTAEHALRALSEMASLEEGKVILGKDLARSAGIPSNYLSKV
                     LWVLGNAGIIDATRGSGGGYRLRRKAADIRLSEVVKLFDRQRPECRCFLGGTRECSDL
                     DHCGAHEEWRRVKESFLGFLEKTTLADLVRRNGERGMSVTLQRPPKSFKQRSK"
     gene            complement(21809..22267)
                     /locus_tag="VLE22_18110"
     CDS             complement(21809..22267)
                     /locus_tag="VLE22_18110"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSB16370.1"
                     /translation="MLSATSQYALRALAHLATLKENETVLGRDLAKKTGIPQNYLSKI
                     LVTLGGAGIIRASRGTGGGYRLARRPEEMSLAAAVALFDKGFLKPACLLGYNQVCSDE
                     NACPAHQAWRDTKTSILRFLESTTLAEIADRPGPAPGRRQPKRKRSAGPK"
     gene            22414..23472
                     /locus_tag="VLE22_18115"
     CDS             22414..23472
                     /locus_tag="VLE22_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16371.1"
                     /translation="MQRYVPLLLVPAILFSQENFGVLPEGSGLAAKYRGDSGIERDPA
                     VVFMENFRSGTLADLAARWTDISNRNGKVLAFAEDVPTGSASRWSLRMTATRNVDSGG
                     HLYRTFQPGYDRLFLRFYVKFAPDAGFNHHFVSLGGEIDPAKYAVGRAGLRPLNRWNT
                     GIEPAASSQHGDHNAIAPPGVWNFYTYWPEMRSWQSPDGKALDDNGKAFYGNSFEPKT
                     PTPVPRGQWISVEIMVKMNSSPDSYDGEQAVWIDGKLRGRFARGTMRGKWDRALFRVD
                     PLGEPFEGFRWSGDLRAKINKLWLSHYVSSESAFPRTDKYAAEHPGFVVNTQTQTVWF
                     SSVVAATEYVGPLGRLPD"
     gene            23612..24733
                     /locus_tag="VLE22_18120"
     CDS             23612..24733
                     /locus_tag="VLE22_18120"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HSB16372.1"
                     /translation="MTRIILSLILIASGTFAAERRAKNVILFLGDAGGIPTLHAASLY
                     KHNHPQKLFLQSLPYIALSDTSAADAWVTDSAAGMSAIVTGQKTNNGVISQSAAAVRG
                     KQDGEMLQTILEHAEQRGLSTGVLTNMVITDATAAACYAHSNDRSRAGEIFAQVLHPR
                     FGDGVDVLIGAGRDRVIESTAKLGIQIESALREEGIAFRDSLAAISDQDRRVAALFNT
                     HDFDVSDAVRRAIRILSKNRKGYFLMVEWDAHTDNLRRGLEQVIQLDAAIGETASMTD
                     PDTLILFTADHSFDLRVRGGKPGQPILPEEGAGAAAKARPTIRVDDGHTGEQVLVAAQ
                     GPGAERVHGFLTNVDLFHIMMAAYGWDKTGAPSGGRAAR"
     gene            25067..25792
                     /gene="ric"
                     /locus_tag="VLE22_18125"
     CDS             25067..25792
                     /gene="ric"
                     /locus_tag="VLE22_18125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HSB16373.1"
                     /translation="MQGIQDATVSEIAASSLAAVRVFERLGIDYCCGGKRPLADVCRE
                     TGRDLPSVLSDLKSAMSATQGPARDWATALLRELIGHIVGTHHEYLKRELAPIGARLD
                     KVYRVYNERYGPTLTALPEVFNALRSELEMHMRKEEMVLFPAIASYESAAVAGSPLPG
                     TPFGTVSNPIRMMEAEHDSAGTALADIRAITASYAIPEYACVTYRSLMAGLQELEQDL
                     HMHIHLENNILFPRAIAMENAGR"
     gene            25945..26838
                     /locus_tag="VLE22_18130"
     CDS             25945..26838
                     /locus_tag="VLE22_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16374.1"
                     /translation="MRLTSLLLTTILMVLVLSSCTDKSAVELSERRHATVFMQDGTKV
                     AGTVVASSASEITLAGDDNITRTIPMKQVRSVEYSDEPVAESQAPAKREPEPASPARR
                     SAPAPGRQSAAPAAQSAPRSRTYELAAGTDIPVRTNETIDSSTAAEGQSYAAEVSQDV
                     LDASGTVAIPRGSQAQLVIVSASRGGRIRGASDLVLSLGSVSVGGRNYAPETASVQQS
                     GKAGIGANKRTATYTGGGAAVGAIIGAIAGGGKGAAIGAGAGAGAGALTQILTKGKAI
                     RVPAESVLTFKLDQSLRIQAE"
     gene            26845..28776
                     /locus_tag="VLE22_18135"
     CDS             26845..28776
                     /locus_tag="VLE22_18135"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSB16375.1"
                     /translation="MASRLSRASDVLHSLGLLFLAIIPAIGFAQNPFSLSEKDEIELG
                     RLAAGETEKSVLLINDPAVVKYIDDLGQSLVQKSTRKEIAYTFKVVDSPEINAFALPG
                     GFIYVNRGLIEAAETQDELAGVLAHEIGHVVARHGADQAVRTGLVQTGMGVFGGLLGR
                     GTSSSLGKIAAEMVATGVFMKFSRQAERQADRLGARMVYDASLQPQGMVSFFNKLAGL
                     QKSQPNAVLKFFSSHPSPAERSSNVSNLIASYPASSKPDGDSPVFRDVKQRLAALPRP
                     EVEAAKAAAAIAAAAQPPAEPETDVRSRNMVRDRDVAARFAPVFHQALGPAPRFDYIT
                     NFDFDGDWRGDNNWNNAENRSYPLKAYVYYAVFETRTHFFIHYAAFHPRDYKGGEKRG
                     ALLSEVMRIGVGLGGQYDPTGRSNEAVMAHENDLEGALVVAEKDGEDYTKAQLTLVET
                     LAHNNYLKYTPPGSSLPGDPIIVVGVRPRLFVESKGHGIEAWRDDADQHKMAENGFLV
                     YVFTAAAEDPDEKPDRAVGYELLPTYESLWPHARSGKNETCGEEHDYGVVSAKFAQCN
                     EDVLRHIRLGVMGSALNGVVGAANMSRPPWGWFDGQDRARPLGEWFLQPAETIRRRWN
                     LPESFSTTYTYHPFLGQFR"
     gene            29002..30105
                     /locus_tag="VLE22_18140"
     CDS             29002..30105
                     /locus_tag="VLE22_18140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HSB16376.1"
                     /translation="MQHSKRLCVLLILAGALALAPAALAKQGFVQHNLVSDVEGLADR
                     TDPNLVNPWGIVHSATSPWWINANGTGLSVLYDGNGAAFPAASPLIVTIPGLVGTSAP
                     TGIVFNGTADFEVATGLPARFIFATEDGTISGWNPTADPTHAILKANPPDALYKGLAL
                     GSMGTQNVLFAANFRNGTVDVFDTNFNLVSMPAGAFVDKNVPQGFAPFNVVNISGVIF
                     VTFAMQDEEHEDDVAGPGLGYVDAFSPDGTLLMRLEHGRWMNGPWAVVLAPQGFGKLS
                     GRLLVGNFGSGQIASFDPESGEFEGMLRGLRGKPVTIEGLWGLGFGNGANAGSATTLF
                     FAAGIEDEAHGLFGTLTAVKNNNEDDQGEDDNQ"
     gene            complement(30186..30461)
                     /locus_tag="VLE22_18145"
     CDS             complement(30186..30461)
                     /locus_tag="VLE22_18145"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB16377.1"
                     /translation="MAERASSPARGRRHVAASELKRLAPIFVTALATSLALLPIVLAG
                     STPGQEIEYPMAVVIVGGLLTSTLMNLLLMPALYLRFGVASHQSPGA"
     gene            complement(30653..30973)
                     /locus_tag="VLE22_18150"
     CDS             complement(30653..30973)
                     /locus_tag="VLE22_18150"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSB16378.1"
                     /translation="MKRILIALAVALAFTLACAAQAPKKGEVKKGAAVAQQVATKKAA
                     DLIDINTATADQLKAIPGIGDAYSAKIIGGRPYKAKNELVQKNILPQHVYDKIKNQII
                     AKQK"
     gene            31099..31941
                     /locus_tag="VLE22_18155"
     CDS             31099..31941
                     /locus_tag="VLE22_18155"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB16379.1"
                     /translation="MAGKSILIVDDDPAIHVLLRQLLNAEDWQVDSAIDGDEALSRLL
                     AGAYDLVLADIVMPGMDGLALLKRIREVRPLVKVVIMTAVNTPAHVVQSIRNQAYSYL
                     GKPFSREAVSETLHAALVDAVGEDDIVVHSAQPNWVSVELRCKLSTAERLTQFFRELS
                     ADLKTEDREMISTAFRELLMNAIEHGGRCDPGQKVNLTFVRGARCILYYIRDPGEGFS
                     FERLPHAAVSNSPERPLAHAEVRGALGIRPGGFGILLSWNFADEVVYSERGNEVVLIK
                     YLNP"
     gene            32135..32746
                     /locus_tag="VLE22_18160"
     CDS             32135..32746
                     /locus_tag="VLE22_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16380.1"
                     /translation="MNTAEGNNAKRSAAASEPAPDSGKSRSPVSEQAPGNVDKIRDIL
                     FGSQMRDYEVRFARLEESLRKESSDLREGTKRRLDTLEGYIKKELESLESRLKSEKQE
                     RAESHGAVSADLKSTAASLTKKISEVENQNGQAQRELRKELLEQSNKLSDEIRVKNEE
                     LASLLDRRVQELRNDKTDRTALANLLTEVAMRLTDEFHVPGAE"
     gene            32768..34339
                     /locus_tag="VLE22_18165"
     CDS             32768..34339
                     /locus_tag="VLE22_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16381.1"
                     /translation="MSSEGSAIHQGSADDEFAELSAILLAAERSQLSELKHRLEDPEA
                     RAQDVALVLAEAIRLRAAGDTKLRRALQPTIEEAIGVAVRKNPHMLASALFPIFGKAI
                     RKAIASELDGMIQALSQILEQSLSLRSLKWRFEALRTNKSYAEIVLLRSVLYRVEQVF
                     LIHRKTGLLLLHAQSGSSGLKDPEMISGMLTALQDFARDAISGAEGENLETVRVGDFT
                     VVLAYGPELILAGFVRGVAPRGLNRLFQDELDSIQEEQSAASVDFSGDTSKFESSRPH
                     LEKCLVGQGDPRAKAPASSLAARLLLFGIPALLIAVALGWWLVSLRAERRWQSLVERI
                     SSQPGIVITTSGEHGGKRFISGLRDPLAADPAAMVSAAGLSSSQVEFRWEPYHSLHPR
                     FAAERQYSAVKEQLEGSAIRFSLGSAAISAEAGHAVDELASKVTALFNAAATTGREVK
                     IEILGSTDFRGRSELNVNLARDRAATVEAALAGLGVPRERMEARGLTSPCGLARGRDD
                     AACRSATLRVLEVSR"
     gene            34343..34873
                     /locus_tag="VLE22_18170"
     CDS             34343..34873
                     /locus_tag="VLE22_18170"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HSB16382.1"
                     /translation="MIKKKICMVGSFGVGKTSLVKRFVESIFGEQYLTTVGVKIDKKD
                     VVVDGCTVTLAIWDIAGEDDLAEFRISNVRGASGYVLVVDGCRRASLEKAVDLQRRIT
                     ASFGLLPFVLVANKADLRDEWEIQPDAIAALIGEGWHAIEASAKTGDAVEQVFHTLAK
                     DMLDRDASRVVEGDEE"
     gene            34883..37201
                     /locus_tag="VLE22_18175"
     CDS             34883..37201
                     /locus_tag="VLE22_18175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB16383.1"
                     /translation="MLDGPRTFRAGMDLLPLLGFAVFERAEAGSFRPAGQPPEWLRTF
                     PAEAQTVCTTDALVEIFPYLDVFLPDAEEHWNAGKPARLHSEMWTQTDLEGKEWRLRA
                     TATCLEARALLVIEPAEVLFNETQSFVQHAHDATLAYDKVAKLSRALKSANEQLEIRN
                     RDVERATNAKSEFLARMSHEIRTPMNAILGMADLLWETPLSPEQREYVRVFRRAGDNL
                     LNLINDILDLSKVEAGQLELEQADFDLSEVLEKAAEIAAVRAHAKGLELSCRIKADVP
                     HLLTGDAGRLRQVILNLLGNSIKFTERGELTVWVELDPDRCEPGALRFRVEDTGIGIA
                     PDKLATVFENFTQADSSITRRYGGTGLGLSISKRFVELMGGRMWVESSLGIGSVFYFT
                     TVFKVRNVQPSPPPEFTGLRCLVVDDNANHRAAVCDILTSWGAVVGQSDGPDAVARLR
                     AGMQSGAPCSLVLLDGVIPGIDSFHLAKVIRDSMLSSQVLLMLTTDRPADAARCRQLG
                     IRTLLKPVRRSELIDVWRTGAPLQPVTTPDSVPTVPEFDSMRILLADDSDDNRFLIRS
                     YLRNSGCLLDEAEDGLAAVQKFRAAKYQLVLTDVEMPVLDGYSATRQMREWERESGRR
                     PTPILALTAHALTAEAQKSVDAGCNAHLTKPIQKSVLLEAIRQYALREASETIRAVVD
                     SSLREIVPGYLTNRRMDVIAFRTALEHDDFQTIRMTAHKLKGTGGGYGFPMLTQLGAA
                     IENAAITRDAESIRKNIAELDRYLDKVEVEYH"
     gene            37219..38541
                     /locus_tag="VLE22_18180"
     CDS             37219..38541
                     /locus_tag="VLE22_18180"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB16384.1"
                     /translation="MKVRFWGTRGSIATPGIGTTRFGGNTSCVEAVTDSGVRFVLDCG
                     TGARLLGSHLAASAPRPIGANILLSHTHWDHIQGFPFFVPVFVPGSRIAVYAPQGGGR
                     SLSAVLAGQMEFTYFPVELDQLSAEITYHDLAEGTHVIDGVTVKAQYLNHPAMTLGYR
                     IEADGVSIAYLCDHEPFSTTLWQTDSAPGKIKSILHDGDRRHAEYMSGADLVIHDAQY
                     TPEEYPAKKNWGHSTYEYVVEVAAAAGVESLVLTHHDPAHDDVFIERIQDLAASVATA
                     RGSSMRVSCAYEGCEVVLRRGESREAAVVQPGSAPSAEGGRILIVDDDPELRALVRVT
                     LVKGGFTVSEAASAAEALQMVGENPLVLIMLDVLMPEMDGLELLRRLRELPAARNLPV
                     LMLTSLNDAESIRKGFDLGCTDYLSKPFSMPQLTSRVRACLARAAGVA"
     gene            complement(38592..39692)
                     /locus_tag="VLE22_18185"
     CDS             complement(38592..39692)
                     /locus_tag="VLE22_18185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB16385.1"
                     /translation="MRILFLEQFSSLGGAQRSLLELLPAVRRANWEAHVGLPDDGPLA
                     ARLREQEALIHVLPLSQYSDGPKHLSDAARFLRDLAVLPAEIRKLDKLLEPAVLYVNG
                     PRLMPAVWRAGLGRPVIFHSHNYVSIWNGKPLVAAALNATRATVLAATRFVAHQWGAR
                     ARVIYGGVDGPPPDWRRAARASGPRIGMIGRIEPDKRQKEFVLAAAELTPEMPDAEFL
                     LCGDARQGDEAALRYKREVLASAPASLKYLGWSDDVYEVLSKLDLLVLPSRCEGGVPY
                     VLLDAFSAGVPVLASPVGDLAETIRDGENGFLLHSASPSGIARRLRELLSMPERLALA
                     AGNARRLWRQRFTAERFRQEMWEVILATARRN"
     gene            complement(39673..41130)
                     /locus_tag="VLE22_18190"
     CDS             complement(39673..41130)
                     /locus_tag="VLE22_18190"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSB16386.1"
                     /translation="MSERSARRELALGLLLGGGAGLTAVALSAASGLRNVLAFTIAAV
                     LVAAAWWMLNGASRWVMVFLGMALLLPPLPLALGNAGPHPSLFAAAIGVWAGLARLGA
                     WTLRRRSIALALVSLTAVLLLSIPMAAIYSGWRLAFESAARICLFLISVYSFFYLADG
                     PGRELPPEWLVRLLFHTGAFGAIFACLDFYFQFPAPVRFAEQFVWLPGGVFRRAQGVF
                     YEASTLGVFCVFLLVMLAAVAVLKLGPALRLTSAWLLTGGTVILAALVLSFSRAAMVS
                     LVVSLGALLWLERENLRIVRTVIRLVFLALAGILAGGAALYAFLPAFLTAYVERFWYS
                     GVYLLESPNLILSRRLESWGFLISYLHEHPWQAVVGIGYKTLPYTGYLGRPVVADNMY
                     LSLLIETGVLGLVSLLALHFAVLVSTYRWVKSSQRSGQNDMRRLFGLWLFAFWCGFAV
                     QMSSGDVLTYWRVLAAIVAALAVGVREAENANSVS"
     gene            complement(41127..42446)
                     /locus_tag="VLE22_18195"
     CDS             complement(41127..42446)
                     /locus_tag="VLE22_18195"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HSB16387.1"
                     /translation="MSTAFGTSSARISEWKVVRGGLVAGAAIFGGQLLGFVRQVVIAY
                     LLGTGPPADALSVAFAPVDLWWAVLATTVVFGFGPLLALPEQTPAFSDLARPIFRLAV
                     AVSVLFIFAAEPLVRLLAPGLSAETISQAAQLLRVTATAIPALSLSTLFTALLYSKRR
                     FAFAAFHQGMVNVSTVVGALLLHSWIGAAGFAAGYAAGAWLQLAAATSISWPILRGRT
                     AAHRRMGLRELILGPGPVLSYSLLIGLSPVVTRALASTFGSGSTVAFEYCLKLTGVPL
                     ALLVNSLSSSLMSELAPFRLRKNRRLAVGVIGRAAILSALAALAVVLLMIALAPLVVS
                     VLFERGRFSAASTRTVTDILTGFCPVLVAWAALDVISRSMFSLGEWRIPSICAGLALL
                     INAAGSLSGMISSAQWIGLPVVAGFSTAAAVAAVCLWRLGIEPKRAS"
     gene            complement(42406..43554)
                     /locus_tag="VLE22_18200"
     CDS             complement(42406..43554)
                     /locus_tag="VLE22_18200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB16388.1"
                     /translation="MKIALVHDWLTGMRGGEKVLAEFIRLLPDADVFTLVWKRGNVTA
                     EIERRVRGVSLLDKLPLARQAYRYYLPLFPIAARSLDLSGYDLVISSSHAVAKGVCVP
                     AGATHISYIHTPMRYVWDNREDYFRFGSGRLWKRAALALVAPYLRRFDRRTALSVDWF
                     LANSETVHARISSIYGAGATVVYPPVDTDFFTPSPDMRPAGDYYLVVSSLEPYKRIDL
                     AVDAFSGGPRRLLVAGKGTLGKDLRARARPPVEFLGEVSDERLRDLYRNCRALVFPGR
                     EDFGMVPVEAQACGRPVVCFGEGGATETVVDGRTGVHFRPQTAGSLLAAVEKMETMEW
                     DSRAIRLQSLLFSRHRFRDSVNAFFRAQVPFVLSSNEHCFRDLLSADL"
     gene            complement(43551..44678)
                     /locus_tag="VLE22_18205"
     CDS             complement(43551..44678)
                     /locus_tag="VLE22_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16389.1"
                     /translation="MPDPFDGRQYLSHIRSRWRLAAVVLATALASTLIISLLLPKKYT
                     ARVLLVIEPPAGSDVRASTAVSPVYLESLRTYEHLASSDQIFAKAAERFRLRANTSKP
                     LEKLKNEVLRVSIVRNTKVMEITATLPNPNQAHDVALFVAERTIKLNRESNRADDEEL
                     IAVARTGLDEATRRLAAAGAAFLEARKRASSSEALRFELDRIGDLRIEIARLALTSEL
                     AAADEEERRKTVLTADGTQIEGAQSHLQSLRNRAARLRREEANLERKSADMQKSLASR
                     EAELQASETEFDDARAVREQAQKRLLELQSASGLRGERLNILDPGVPPERPSSPNIPL
                     NLFVAGALGLLLALLYLTIEFGLRSAPVEPVGERRWVAPRT"
     gene            44914..46767
                     /locus_tag="VLE22_18210"
     CDS             44914..46767
                     /locus_tag="VLE22_18210"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSB16390.1"
                     /translation="MPGFAIVAAVLALAPLLILPGSLLYFDVTPKVAVVVLGTAVVLL
                     LGSGPPLERTGATGWFGMLLVVQAASLLLSTALSTQRWLSVTGTNWRRFGLVTQLSVV
                     VFVWLVARCMGASPAVRVLHILRLIAACGAMAAFYGILQYFGWDPWLPASFYHVGEGI
                     WTIVRPPGTLGHAVYFANWLLYALFAGMGLLLAERARTWRLLGAAACAMCSLAIVLSG
                     TRAAVVGLAGGAVFLLFRVRPRLRLRALGATALLLAGLAVFYFSAPGQKLRARVRWSF
                     EDPRGGARLLLWRDTLRMAGEHWAVGTGPETFSAQFPRYQSADLARAYPDFYHESPHN
                     ILLDALATQGILGLAVLLAFCALAFRTGWRAHITYPAVSGVLGAMIVAAIVSQQFTVF
                     ILPTALYFYLTVAMLIALSAGDGTGKTASPGSVYRVVSLVVRVSAAALFTLFAVRLIV
                     ADRALALTRTELEAGRLDVAIEQHRRALEWQPPGMNAELWYSRSLAALAGNSPNLLVR
                     LQAMHRAIEAGERSTRSSEEPPNAWYSLAALYAVQNDAAGVERCLRNAISASPKWYKP
                     HWVLAQVLARTSRLKEARKEAATAVDLNGGRNPEVGRTLDAIAASPDPPNR"
     gene            46872..47684
                     /locus_tag="VLE22_18215"
     CDS             46872..47684
                     /locus_tag="VLE22_18215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16391.1"
                     /translation="MRNRGLVLLTLTCAVAAYSQTPAVFDGGVVNAASFASGQVVTPG
                     SLVSIFGTELTGALALADSIPLATQLLNVSVTFNNIPAPLVAVIPGGSAQINAQLPWN
                     VLPAGEKTGTANVVVTRGGVSSAPKVVQVGPFSPGIFAVNFGIGPAIAILPDGALAAP
                     AGSIPGLATRPAKVGDVLEVLATGLGEVDIPVANGHNSLDHLRQTTTKPTALVGGVPV
                     KVEFSGMSPQFVGVNQVNIRIDNPNVPKGNAVPLQLQVGGITSTDKVTIAVE"
     gene            47688..48698
                     /locus_tag="VLE22_18220"
     CDS             47688..48698
                     /locus_tag="VLE22_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16392.1"
                     /translation="MRGTPGPVLSVEESVETETRIFDPFGQGTDLAHTANFHPLGFPL
                     QLATNSADVLTAARENWSGFIPCFDVPPLRLHVAVDEENPGPCPTSLIFRAQRYLLTV
                     ISDPSNFAVCDLERGFASCWFTRATASNREWLRYFYLDTVAYLILWHQHLTRIHASCV
                     ARNGRGVLLCGPSGAGKSCLAYACTRRGWTFISDEATSLMRRSSERVVLGNPRRMRFR
                     ETAADVLPELSGRVAAPNPAGKITIEVPTSDLPGIITAFQCRAAAVVFLDRHPGGPAR
                     LAPLSPEEGWSRLDQDLPLFDHSVHLEHRSSLKRLLETGVYELHYCDLDRAVELLEEL
                     VR"
     gene            48710..51907
                     /locus_tag="VLE22_18225"
     CDS             48710..51907
                     /locus_tag="VLE22_18225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB16393.1"
                     /translation="MILILLVLAAAFPVWGQIASGSVLGEIRDESGAVVPGARVTARH
                     EATGFARSVFTGPEGVYQIEQLLPGPCTVTAEKFGFQTVIAGPIVLEVNQRARLDLVL
                     KVGIERESVTVTTAVSPLRADDPSAGHRLDAASISSLPLDQRNVISLASLGPGVIPRA
                     LGGFVHDIITDVQPGRGAVALNPSVNGARSPANAFLLDGAADTDRLAFVISVVPPMES
                     VQEFRMQTSLPSAEFAQTAGGVVDVVTKAGAREWHGNAFEYFRNEALDARNFFDDPSL
                     PRPIFRQNQFGGSLGGPVPLPSTFFFAAYEGLRGKSAKSSLNLVPDTTVRGGDFRGRN
                     PIFDPLDRDPVTGARQPFPGNVVPLERIDPVARRFLDTYQPLPNRSGSSGNYLDATPN
                     ENTDDNVSARIDHELHGGSRVFGRYTLNEQRGRFAGSFPVRPTEERIRAQQAALGLTF
                     GRSSWLNEARVSFTRLRVFDVPESAFKTNVAKELGISGIPDDPFTYGLPYFLITNFST
                     VTDSPTLPLTQRDNLWHFSDEVSVARGRHTLRFGFEWVRFNLNYLQSQLARGQFIFTG
                     ALTGDPSAPGTTGDPLADFLLGFPQITSRNVGTTLAYLHNNSAAGYVQSDWRWTSRLT
                     LNFGLRYEYTSPFTEGRDHLQNLDYSTLPAAPRLVPVKYAVDPDRNNFAPRVGLAFRP
                     LPGRELVFRAGYGIYFSQEMAIESYDLIRNGVRNENNVTDGITPVLTLENGFPQTAST
                     GLPGYFGIDRHARTPYVQQWSAGLQKQLPEGIVLDVSYVGTKGTRLGRFRRFNTPLRV
                     ETGENLPPRPGDLQQLRPFPELGEIIQRQHISNSIYHSLQVKVEKRMASRLTFLSSFV
                     WSKSIDDADSVIPGFFESFGAQDERNLRLERGLSFFDVGRRISAGFVYALPASAFLRP
                     VLSGWGMSGIVTLQDGTPLNPVYFAFDPANSGTPNRPDVVPGVSVRLPRSQRTADHFF
                     NTDAFRAPAPYTFGNAGRNILPGPGNNVFDLALHRQFPVRESKAVEFRAEFFNAFNHP
                     NFGIPGPYPDFGPFFGKIFATGQPRRVQLALRIDF"
     gene            51909..54335
                     /locus_tag="VLE22_18230"
     CDS             51909..54335
                     /locus_tag="VLE22_18230"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB16394.1"
                     /translation="MKPILWEENRRVPAGVRAALAALRFAHPDTRGLQVLCEREWKEA
                     LHFCDRSQTTLFLWERCEEEFPEWVRERVGQNLADNTERNTRIREDVFKICCALDGSR
                     TEYVMLKGLTQWPRYVADSRLRVQYDLDLYCPPDSLYRARDVLLELGYEPAEGFENFP
                     VDHLPTMIRKTGFEWHGNYFDPDVPVCVDVHFRFWDEETERFAAPGLDEFWERRTKHP
                     VDGRCVPALDPADSLAYTSLHLLRHLLRGSLRLCHVYELARFLETNADDRAFWEKWRD
                     LHPPGLRRLEAIVFRLAAEWFDCGVHETAEAEIAEVPKGVQFWFERYAASPMEGYFRP
                     NKDEVWLHLCLLDAGKWSVLKRRLLPSRLPARAEPGNLPASEITPRLRWQARARYAGH
                     VAARAMHHAVAFAPALWKGTRWWLEAAGPGAAYWRYLLTASLYHLGVFVFVLLYNLYL
                     LDRGFREDFLGLITSAATIGSLAGTLPAAWGIQRLGLRKTVMACIVGTAVVSAARSLV
                     LGPASLVATAFLGGALSSVWFVSVAPAVSGLSSERNRPVGFSLFFATGIAVGVLGGLA
                     GGRLPGWIQETFALGTAASAKQAALLTGCAISALAILPARRLSLPTGVARESRVYPWN
                     PVLGRFFAAIAAWSFATGCLSPFFTALFSRHFNVSDEHIGLVFSGSQLAQVAAVLAAP
                     LVLRRFGLVGGVVAMQVAAAAMLATLALGPPVLAAAAAYVGFMSFQSMCEPGTYSLLM
                     NQVGEEQRSGASALNFLVIFSAQAAAAGLAGIVITRLGYPAMLAAAATVALGAALLFR
                     RLLLASDSRR"
     gene            complement(54377..56443)
                     /locus_tag="VLE22_18235"
     CDS             complement(54377..56443)
                     /locus_tag="VLE22_18235"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HSB16395.1"
                     /translation="MPKSLLLACLMPVWLLAAAPGSHKPLLPRPQEVRYGGAELPVRG
                     LSIRFASAPGAEDRFVAQELSAALSSIAQTQVPVLDVERSGKTILLSRTGDVAALPMA
                     DERPGPDSREAYSIKINSNGAEIRARSSAGLFYGVQTLRQLVEGEGAHAVLPEVEIRD
                     WPSLAYRGFMMDMSHGPLPKEEEIKRQIDFLARWKVNQYYFYSEVSIELTGYPLFSPK
                     ARYTREQVRHIIDYARERHVDVVPCLEYYGHLHDVFQIERYADLAALPHGGEFNPRHP
                     RVRELLADWVAQMAGLFPSPWFHIGLDEPFELEAAGSQAAGGVEPAKLYIELLQFVSS
                     LVRKHDKRVMFWADVATGSEIFVRYPQLVSELPPGTIAVAWDYEARKDYTSFVKPFSE
                     GRIPHVIATGLWTWNEVVPDLNRTFANVDGFLAAARRHGSMGIIHTGWHDAAQTLYRQ
                     ALPGMAYGAAAAWQSEPVDHSRFFSDYAAGLYPEAIASDVASALEAINKSRQHLEDAL
                     GRDTMHRLWEDPLVPDRLKRAAPHRAEFREARLQAESAQELLMRALEIKPAAPALSSL
                     LLGARMLDYLGQKYLYALEIEGYFTRLGKNPKSSDLYLFLGFEASYQDHSRAIDLMDA
                     ISEMREDYRTAWLEEYHPFRLRRALGRWDAEYEYWRRLQERLSEFVSGFKDNDTLPPL
                     ESFRPR"
     gene            complement(56487..57821)
                     /locus_tag="VLE22_18240"
     CDS             complement(56487..57821)
                     /locus_tag="VLE22_18240"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSB16396.1"
                     /translation="MPTNDQNRLKYALASDVMPGGVNTSLRRFEPHLVFARAEGATMT
                     DVDGNQFIDYQAAFGPIVLGHNHEMVNRRVQETMQNIDLLGVGTTESEIALARKISQH
                     VPSAERVLFTNSGSEATYQAIRLARAVTGRKKIIKFQGCYHGWHDAVAMNVITPAEQL
                     GSRQLLSAGSLPEVVEHTLVCTFNDLDEVERTVAANKGQVAAIILEPIPHNIGCVLPK
                     PGFLEGLRELTRLKNIVLIFDEVITGFRHGLGGYQKICGVTPDLTTLGKAVANGYPIS
                     VICGKRELMDRFNTHSDGDVFFAGTFNGHPVCCAAALATIEVLEQESTYRHMFGLGER
                     MREGLSGIVTRLGLKATVTGFGSVFLTYFMDGPIESYTDLTRNDAEMFVSYRRKLLER
                     GIFKLPMNLKRNHISLSHTAEMVDRTLQAAEVVLRELSRSAKKGAPAMEAAS"
     gene            58093..59529
                     /locus_tag="VLE22_18245"
     CDS             58093..59529
                     /locus_tag="VLE22_18245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB16397.1"
                     /translation="MCVFLQAATLACTSGSTAAQQGVRAAPLPDLAPLEQLKLDEFEP
                     AVGEQIRGAYERASQHPRDAAAAGKLGMILQVYGKYELAERCFLRAKGLDARSFRWTY
                     YLGNVEGFLGKHPDAVQHVREALTIDGQYAPARVRLAQLLLDSGDLEQAGQLYREAAD
                     QNPELASAHFGLGQVLAAREELEAAIESYKRACQLAKNYAAAHYALGMAYRKTGDLAN
                     ARTHLELYQRLKQAKQPVLDPLMEEVNALYSGGLTHFAKGSALAQQGKLQEAAAEFEA
                     ALNVNPGMVMAHINLIAMCGQLGQPEKAEQHFRAAVELDPGWVESYYNWGMFLLQKGK
                     KAEAAEMFRKAVAVNPSYPDAQVQLGSLLDESGRAAEAAAHYERALAADAGHRQAHYL
                     LAHNLVRRGQVDDAIRHFLETIKVEDNRTPFCMYGLAVAYERSGDRQQALYYMEQAHR
                     RAVSFGMRDLASRLQQDLKRLQAEGVRR"
     gene            59625..61313
                     /locus_tag="VLE22_18250"
     CDS             59625..61313
                     /locus_tag="VLE22_18250"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB16398.1"
                     /translation="MATAADVLFEDAAEKTGLRFQHFIGATGSYFVPEIFGSGVALLD
                     YDGDGDLDVYFLQGAVLDKTKSLKDSLFPLPKEHFPGNRLFRNNLIPTGTLSFTDVTE
                     QAHVAGNGGYGMGAAVGDYDNDGHPDLFVTNYGRDILYHNNGDGTFSDVTKEAFPAEE
                     SFGASAAFVDYDRDGFLDLVVVRYNSFTVQGNKRCSGYAGAREYCGPGDYRPIPDKLY
                     HNDGKGRFTDVSQKAGLGAAFGNGLGVVCADFDGDGWIDILVANDKTPNQLWINHGNG
                     TFEDDALMSGTAFDDEGKAHAGMGVTAGDFDGDGDEDIFIANILGETHMLYLNLGNGI
                     FRDVTKLYGLGYTTVPYTGFGTLWFDYDNDGRLDLFIADGEVRAIESQRGKPFPYDQK
                     NQLFHNEGKTFREVTGEAGPVFDLSEVGRGAAFGDIDNDGDIDIVVSNANGPARLLLN
                     QIGNRNHWLEVRLQGVTVNRDALGTRVALLRQGRPALWRRVATDGSYLSANDPRVHFG
                     LGPDAALRVAPPENIVVIWPDGSKETWPIPGVDRVLQFRQGTGTQVKETPTTKP"
BASE COUNT        12202 a        18471 c        19283 g        11603 t
ORIGIN      
        1 cctggtgagt ctcgttgcaa cgggaaatgg catccagttt gccggtatat gtctgacgtt
       61 gccggcgtgc cagcattgga ggttgtttac ctttctatgt ctgagagtgt tcgcgaattg
      121 attaaacagt accaggcgtt ctgcgaggtg gcgccctact acgttgtgtt cgaggagcgg
      181 tcgtccaatg gtgccatcac gaaacaaagg attcaggcgg gcctcgatat tgacgtctat
      241 ggcgtcaaga gtgggcttgg gcccggcccg ttgtctgact atgcgctagg ttgcgctgcg
      301 ttacgagaag tggcccagac ggtgacgagc cagacaggcg attcctgctc gatcgaagtc
      361 attccgtttg cctccaccgt catcctcgac acgagacggc actttcaacc gcaaggaatg
      421 atccggatca ggatcacccg tggccggggt atcggtcaac cggcgggcga gccggaggag
      481 aaagtgctga aagcaatcct gacgcagctt aacgatttgg gaatcagctc cggcaatagc
      541 cgtacgtaac tgcccattgt ttatcttcat cagccgcgat ttgtttgcgt gaaagaattc
      601 gccgggcggg tccagactgt cgtccgacta cgtggacctg cggcttggtt caaagatcgc
      661 gaagggagtt cggcggcagc tccgccgaca aggctctctg tccggcccga tgcggcaagg
      721 ccgggcaacg gacaacaatt ccgaaaacca ccgccttggg ttcccggatt actgatcgta
      781 acgagcacta tcagcccgat tctctttgac ttaatccttg ccttccgcgg tgcagctttt
      841 ggagcagatc agcagcgccg aattgggctt tcgaacctcg cgaacgcggg acgtacgaat
      901 gaagcgccca ctcggatgca ggagcacaag acacaggcct ggtccgatgc agccgggcaa
      961 caacgtggct gttctcgatt ctgaaccgta caacgtcacg cccacgggct ccgggcagta
     1021 ctttgggtgg ccactcacaa gtgcctcaca tccttcgcgc gtttcgacca gatactgacg
     1081 gttgacagtt tgcacgatga gctgcgtacc ggcaggtacc cgatcgaggt acactccgtc
     1141 ttcgacattg gaggtcgaaa tctgtcgcgg gtgttgggac aagtgcgact tgaagcgatt
     1201 gaagaaatca aagatacaaa ggccaaacac ccttttgggt cgcatttgga gtcgtcctgt
     1261 tctgagccat accatcgggt tcgttccctg aatcggagta tgctcaactg cagaacagca
     1321 atccgaattc cagaggacgg ttcgcggagc cttgagagcc ttggccccga gatcttcggc
     1381 gccgaaccgg caagacagat ctgccacgct catttccgtt gcgacgtagc tgctttcgaa
     1441 tcgatccatg gtgaaacgcc tgtttggcac ggctcagcga tccagtattc actcccagat
     1501 gtgacgacag gggtcgccgg gcccgtacgt cgccgatccc gggttgcact cggaatccgg
     1561 ccgtgacgcc aaagccgaca ttcgctgttg gcatctgcca atctcggcac ttctcttttc
     1621 ggaactcgtg gtgccgggca gcaaaaacgg agtgggagca cgttcactgc tcggttctta
     1681 ccagttcccg tgcgagcctg gacggtactg ccaggcctcc gccggccaca ccagtcaagg
     1741 atgcaaaatg aatcgcaatc accttacctt ggcggtccaa caccgggctt ccactgcttc
     1801 cgtcactggt cagtgctgag accgtaagaa catcactggt aacattgctc aaataaccta
     1861 ccgcgacgaa cggctcgatg gggctccgca tgggcagcat ccctggaaat gccaagtctc
     1921 ccttgccccg actgtaggga cttttgtagg gatactcctt caaaatcggt agcaaaacac
     1981 gggttcagct gcgcttgggc ggagttgagg aaatcgaaaa gtgccttgct acgagcaact
     2041 tcgctcaatc ctgatcattg ctgctttcgt cctgccctgg gctgttaacc gaagggttgt
     2101 aggttcgagt cctacctgag gagccattta gtttcaacaa cttccagaaa aagagctcta
     2161 cgtcaaaaac gggggataag ccccgatccc gaacaaatca accacatcat aaccttgacc
     2221 cgacgtctgt agttttggga gttgcgggag ccgctgttgg caggcgacgg tgtctgccgg
     2281 gcggcagaat ggaagatccg cccaacgggg atctcctggc cgggtgggtc gcaagatcca
     2341 attagctcga agatgaggcc gtggcaccgc ccaggccggc cagcgcacga acggtttcga
     2401 gggtgtcggc tgcgtcgggg gttttgtcaa cgcggtaacg cttcacgcgg gcgaagcgaa
     2461 gcgcgaggcc gctttgatag tgcgggctaa tctggatgtc gttgaaggcg atttcagcga
     2521 cgaacttggg ggcgatgtag acggtgtatt cgtcgcggcc gatttcagcc tgcaggaact
     2581 gctcggtttg ccaggcaagc atgtcgtcgg tgaggccttt gaaggttttg ccgagcatcg
     2641 caaagccacc cttgtccgtg tcgcgcgcgc cgaggtgaag attgctgagc cagccgcggc
     2701 ggcggccact gccccattcc gcggcgagaa tcacgaggtc gagcgtgtgc gggcgcttga
     2761 tcttgagcca gctctggccg cgcgctccgg cggcatatgg cgagtcgatg gccttcgcca
     2821 tgacgccttc gtggccctgc tcgagggcct ggcgaagaaa ctccgcggcg cgattggaat
     2881 cggcagtgcg cgtgtgcgga atgagcgcct cggccggcga gagcccggcg agtgaggaaa
     2941 aacgccgcga ctgcggctcg tccaggagcg actggccgtc gaggtagagc aggtcgaacc
     3001 agaacgggct gaggggcagt tcggcgcgca tgcggtcgag accgagcttg cgattgaaac
     3061 gccgggcggt aacctggaag ggccgcgggc ggccttcagg ggtaaggctc aggacttcgc
     3121 catcgaggat gatatcgcgg gcgggaaggg cacgcacgac ttcgacgatt tcgggaaccg
     3181 cggcggtgac gttgttcaac gcgcgagaga agatctgcac gtcgtcaccg gaacggtgaa
     3241 cctggatgcg ggcaccgtcc atcttgtatt cgagcgcagc ttcgccgagg tcggcgaggg
     3301 cgccggcgac gtcttcgtgg ggctgagcga gcatgggctg gatcggccgg aagagctgga
     3361 tgtcatagct gtccaacccg gccgcaccgg attcgagcgc ggagcgggcc acgcgggcga
     3421 tgtcgccggc cagcatagcc gcgcggcgca cccgcggagc ggaaatgcca gacgccttgg
     3481 caagagcttc gagcatgata ccttctagcg cgccctggcg gatctcgcca agcaggaggc
     3541 cagtgaggaa ttgctgctcg ggcgccgtgg agcgggaaag cagggagtga agaaggtcgc
     3601 ggcggcggcg ttcggagccg gaaccctgca cggcggccag ctcggtgagg gcgcggtcga
     3661 gcacgtggat ttcaagcgcg gcggcttcgt tgggatccgc ggcggcggcg cggagcgtgg
     3721 cgtagccgat ccggacacgg ccctgtcgtg tgtatccgga aaggaaggcg acgacgattt
     3781 cgacctcttc cggatcgagc cgatggagga gccccgccaa aaggtcgatt ttttccagcc
     3841 ggcgcgaggt ctccgagacg cgacgagagg tttcgacgac agcgctgaag agcatctgtt
     3901 ccgagtatag atctggcttt gcgagtctag atctgattcg ggatggatcg aaatgggatc
     3961 tcgacggatt cgccgggcct tgcagggcgg cttaacagcc caggcactga gcctagtggt
     4021 ccgacaagta ccgcagtgcc gggaaacaac gctgccggaa gtaatcttcc gtcgtccgtc
     4081 cgccccacgg cgaacacgcc cggcgccgac gacatgactt cgatttccgt caagcgtcga
     4141 gcggccctcc ggattcgtca actccacgtc cgccgtgggc ggccgagaca agggtgagga
     4201 ggagcaggca ccggaatcga ggcaagggag atcacctatt ggcttgtgac gcatattgta
     4261 agaatagttc ccggttgtat tagcatttgt tttaggagaa ggcgatggcg gccaagagaa
     4321 ttctcatgct ggtgggcgat tacgtcgaag attatgaggt gatggtcccg ttccaagcgc
     4381 tgcgtatggt ggggcaccag gtggatgccg tctgccctgg taaacaggca ggcgacgtgg
     4441 tgcgtaccgc catccacgac tttgagggcg accagaccta tagcgagaaa cgcggtcata
     4501 acttcgcctt gaacgcagcc tttgacgcga tccggcccga agactatgac gccctcgtca
     4561 tccccggggg acgagctccg gagtacctgc ggctgaaccc ctcggtgctg gaagtggtgc
     4621 gccatttctc ccgtgccgat aaaccgatcg ccgctgtctg ccacggcgcg cagttgttgg
     4681 cagcggccgg agtgttggag caaaaatgcg tgagctgcta cccggcggtt ggtccggagg
     4741 taacgcgggc aggagggcgg tacatggatg tagccatgga ccaagcccat gtggacggga
     4801 agctggtaac cgcgccggcc tggcccgcgc acccggtctg gctcgaagcg ttcctgaagg
     4861 tgctgggcac tcgtatcgag ctttaagaaa actgaacaac ttcgcggcca gagacggaca
     4921 agcctggtgt agtgcgtcag agatattgtg ccttatgttt cgccgccctc gcaggccacg
     4981 gaaaacgatg gtctgcgcca caactgtccg gggcggtgat aacggaagtc aacaggggag
     5041 tagatacctt tttcagtatt gtcatttgaa acggctgggg ctaaaatcag gacgctgttc
     5101 gagaaacact gcttcgttta cagcgcggtg acttacctcc ccgcgctgct gctagtaacc
     5161 gcccgtcccc ggagggcgca ggagatagcg ccctccgggc cctcccatca ccgccgctcg
     5221 tgagaatcac gtctgacggg actcagttgt ggtagttgac gctttccgcg aggtgcggat
     5281 ctcccaacgg taccggtgga gcgctgttca tcgcccgcga aaaaacgagg atgaagatcg
     5341 cgattacacc taccagcgcg gcagcttccc ataacccgaa ccgcggattc gaaccgccgg
     5401 tggcgggctg gatcatgatg tacaggtcaa gccagcgccc caccaggacg acggcggccg
     5461 ctttggcgag cagacttccc gttcgcttgg tacgcgtggg caacaagacg acgaacggca
     5521 ccacccagtt cagcaccata ttggcgatga acagcggcat ccaaaactga tgctgccgct
     5581 tgatgtagtg cactgtctcc tcggggatat cggcatacca gatgagcatg tactggctga
     5641 accagatgta catccagaac gtcgagaagg cgaacagcag tttgccgagg tcgtgcagat
     5701 gctcgtcggt gacaaagtcg cgaagcggtc cggcgcggcg catccagaca accagcagaa
     5761 tgatcacggc caggccggaa gagaacatgc cggcgaaatt gtagattccg aagatcgtgc
     5821 tcgaccagtg cggctcaagc gacatgatcc agtcgaaact cgccagccag aaggtgacgc
     5881 cgaaaaccag gaggaagatc accgagagcc tggcgtttct tggtgtggcg cccgggtccc
     5941 gagtgtcgtc ctgaagccgc gacgtcttga ggattgccct cgtgaggagg aaccacaacc
     6001 cgacgtagag gatggcacgc gccaggaaga aggggaagct caaccagacc gccctgaagc
     6061 cggccatttc cccgcgatca cgaatccacg gatagagcgg agatccgtaa aggaagacaa
     6121 cggcgagcat ggccgcgccg tacggcagag cggcgcacat cgcttccggc acacgccgga
     6181 aggccgtacc ccacgccgcc ccactggcgt agtggatcgc tacaaagaga atcccggcta
     6241 gtcccaggca caccagaccg tagccggcca tcaacaggga gccgagcgcg cgttggggcg
     6301 cgaggagcag tccgaaggca agagcgagaa agccgccggc cgccatcccg tacaagcaac
     6361 gttcaaacgg gggcgacaat cgaaacccgg tccatagcgt gttcacttct tttcctcccg
     6421 accgttcggc ccggacggtg cggggacagg ctgcgccacg ggcgtctggg gcgtctggga
     6481 cgtctgcgcg ctgcgctgca gggaccgcac gtagagaatc accttccagc ggtcgtcttc
     6541 cgacacctgc gatgcgtacg aaggcatgtt cttctgtccg taagtaagga tgtggaacat
     6601 ccggccatca ttcaacccaa gcgtcttctc gctattgagt ggcggcggag cgggatagcc
     6661 gcgcagcgcg accggaccgt cacccctccc ggtggctcca tggcagggca ggcaccaggt
     6721 acgaaatacg aaggcgccgc gcgtgagagc ggcggggttc ccggcaggat agggattgct
     6781 cagctcttgt ccggcccgaa tcgcatccgc ctcgccagcc tgataatcga cggcatgaaa
     6841 gccgcgcggg atcgtacccg gaggcggttc ctggagggtt ttcccgtcgg ggaagttcgg
     6901 attggccgag tatgactggt aggacgcggt gcggaccatc tcgggcagca cctccagatt
     6961 cggccgggac ggctgccacc gaacgaaaag accgaaggca acgcacagca cgagcgccac
     7021 ggccagcaac aggttgagtt tcacggcaag gactcctccg cgcgctcgat tacctcaacg
     7081 gccccatgcc gctccagcag ccggcgcacc aggaccggct caaacgccgc gtccgtttcc
     7141 tgaagcacca gcgcaaagcg atcgttggtt accccgggaa tgacggacgc cgcccggcgg
     7201 ccgggataga gacggctgac gaggaagaac gcgaacacag tactcatgcc ggcaaacagc
     7261 accattacct cgaaggtgat cggcacgaat gccgggagcg agttccacgg tttgcccccc
     7321 acgttcacgg gccagtcgga ggccgtagtc caatattcga acgcgacctt cgccgcggct
     7381 ccgatcaagc ccagcaagaa gcagacccag ggcagacgcg atggttgcaa ccccattgcc
     7441 ttgtcgaggc cgtgaaccgc gtatggcgcg tagatgtcgc ggattgtata gccctgtttc
     7501 cgaacctcgc gggtgaccga aagaatgtcg ctctcgtcct gaaagatgcc aaccatgacg
     7561 cggccgctca ttggtgattt ccccctttcg agcgctcatg cgccgcggct cccttcacct
     7621 ccgccattgc gatcacaggc aggacgcgac agaagatgag gaaacacgta aagaagagac
     7681 cgaaggttcc cagcagtgtc gcgacttcga aaacggtggg acgatagctc gcccagtttg
     7741 acggaagaaa gtcgcggtgc aaactggtca cgatgatcac gaaccgctcg aaccacatgc
     7801 cgacgttgat cagcaggctg acggcgaaca caagcacgac gttcatgcgc acgcggcgga
     7861 accacaacac ctgcgggacg atcacgttgc aggtcaccat cgtccagtaa gcccaggcga
     7921 atggaccggc cgcgcggttg aggaagacaa attgctcgta tggattgcct gaatactgag
     7981 ccgtgaagaa ctcagtgcca tacgccagag cgacgatgct gctggtgagg atgacgagtt
     8041 tgcacatcac atcgatgtgg cgcaaggtga tgtaattctc gaggttcatc accttgcggg
     8101 cgacaatcat cagtgtgaga accatcgcca tccccgagaa aatcgcgccg gcgacgaaat
     8161 agggagggaa tatcgtggcg tgccagccgg gaatcaccgc ggtggcgaaa tcccagctca
     8221 cgatggtgtg caccgacacg acgagcggcg tagccaggcc ggcaaggagc aggtagacgg
     8281 tctcataccg ctgccaggtc cggtacgacc catcccagcc gaggctgaac atatcgaaca
     8341 ggcggcgccg gattccagcg cgcgcccggt cgcggatggt ggcgagatcg ggaatcaggc
     8401 cgaggtacca gaacgtaagc gaaatgatga agtaggtcga gatggcaaag gcgtcccacg
     8461 cgagcggcga gcgaaaattg atccacagcg gtccgcggaa gttcggataa ggaaaagccc
     8521 aaaacgccat ccacggccgg cccatgtgga tcagcgggaa gatgccggca cacatcactg
     8581 caaacaacgt cattgcctcg gccgagcggt tgacggaagt tcgccagcgt tgccggaaga
     8641 ggaacaggat ggctgagatc aatgtgccgg cgtggccgat accgatccag aagacgaagt
     8701 tcgtaatgtc gaacgcccag cccaccgtat tgttcaatcc ccacgttccg atgcccgtgg
     8761 cgacctggta ggagacagcc gcgacgcctc ccgccagcag ggaaagtgac acgagaagac
     8821 cggcccacca gagggcgccg gggcgcctca gcatcgcagc gcagatgtcg ccggtgatct
     8881 ggccgggact tctatcgccc tggatcagcg gcggccgcgg gacagcctga acttcagcca
     8941 tgacgtccct cccccttgcc cggctcatgg tgccggacaa tcttcagata gcccacggag
     9001 gggcgaacat tcagttcttc gagcacgcga taagtccgct tgctcttcat ccgttcggtg
     9061 accttgctct tcggatcgtt cagatcgccg aacaggattg ccttggccga gcacgactgc
     9121 tggcaggcgg tctggatctc gccatcccgt aaagcgcggt tctggcgttt cgcctcggcc
     9181 cttgcgtcct ggatgcgctg tacacagaag ctgcacttct ccatgactcc gcgcgaccgg
     9241 acggtgacat ccgggttgag cgccaggttc gccaaacgat cctcccgcga atactcaaac
     9301 cagttgaagc ggcgagtttt gaacgggcag ttatttgcgc aataccgggt gccgacgcag
     9361 cggttgtaga tctgctgact gagtccttcc gcgctgtgca cggtggcgag caccgggcaa
     9421 acggtctcgc agggggcgtg ttcgcaatgc tggcacatca tcggctggtg cgccacttcg
     9481 acgtcgtccg gcggtcccga gtagtagcgg tcgatacgga tccagtgcat ttcgcggttg
     9541 cgcagcacct cgtccttacc ggtgaccggg acattgttct ccacctggca ggcaatcaca
     9601 caggccgagc agcccgtgca cgcgttcagg tcgatggcca tggcccagcg atgaccggtg
     9661 aacggatgat cgtcgggcca aagatccgtc tcggacggcg tatgcgcacc ttcatgcttc
     9721 gcctcgccgg agtggagcgc cccaagagtg gtctcctgga caatcgggcg cgggccccca
     9781 cccggatcga gatgtttcgg caccgtgagg ctgtggtgat cctgcgtgac ggctaacagt
     9841 tggcgcttgc cggccgatgt cactttcacg tccccgggcc agtactgaac ggcgccgttc
     9901 tcccagccga tgaggggagc ggcattgacg ccgacttttc cgttcgggcc cagggtgggc
     9961 cgccgttcaa gccacttggg ccctgcgccg gcgaatcgcg cggagaccga gcgcccgtag
    10021 cccagtgcca cggcgacaac ctgctcgtgc tgtcccggct ggatgagaac cggcaattca
    10081 atcgcggttc ctgaagcttc gagtctgaca acgtccccgt tcttgaggga cagccgctcc
    10141 gccgttgcgg gagcgaggct ggcatagttg tcccacaccg cttttgtcac gggatcggga
    10201 agttcctgca gccacgggtt gtacgcgtgc cgcccgtccg gcatggcgac gttggcgtaa
    10261 agcacgagag cgagcccgtt gccggacggc gggacgacgt cgataggctt gaccacgctc
    10321 aggtcgaacg gcttcagcgt tggggactgt ggcttcacca gcgcgaaacc cacttccacg
    10381 gacttcttcc agaaaacgtc gaaggactgc tggcgctcct gccgcggata tacgcgctcc
    10441 ttccagaaac ccttgaccaa ttcgtaggct ggagcgtcga cgccggacca gcgggcgaaa
    10501 ctctcgatgg ccggccgcgc ctggccgagt ggacggatcg caggctggct gatggcggct
    10561 acgccggcga cgggctcgga atcgccccag gtttcgagcg gatcgcgcgc tgggcagaga
    10621 tagcgcgcca gagacgaagt ttcatccaga tgcggcgcca agctcacgag cagcggaacg
    10681 cgggccagag accgcgcaag ttcctcgccg ccgggcaagt ccgccaccgg attcaagcca
    10741 tgaatgaaca gtgccgccac gccgccggcc ttgatctcgt ccacaagggc catcagctcg
    10801 cggtcatttc cctgtttctg gtttgatggc gcatcgacgt ccaacgtgga gccgtagttg
    10861 ccgagtaagt ggttgacaag gttgcagagc acctgcgcgt ccgtactggc aagtccgcac
    10921 accaccaggc tccggccgcg cgcgttccac aagcgtgttg ccagatcgtc catgacgtct
    10981 tccggcgccg gagaccgtga tcctgcgcct ggcaagacgg ccgatccggc tttccgcgcg
    11041 attctcacgg ccagatgctc aagcgcggct gggatttccg ccggggctag tttgatcctg
    11101 cgatccgcct tcgtccccgt caacgacatt cgcgactcga actgaatgtg ccaggacgac
    11161 cgggcaggct gtgcatccac gtcacgcgcc gcgctgtacg ccgccgtgaa ctccacggga
    11221 gagatccacg tgcctaggaa gtccgcgtcg aagctcgcga tgaccgcggc cttcgtgaag
    11281 agatagcgag gcagcactct ggccccgtgg gtccggaggt gagcgtcgag aatgggagaa
    11341 gccgaaagga cgtcgtagac gacatggcgt gcgtcctgga aactagccag gaacgtgctt
    11401 atctgcgagg acaacgccgg accggtgatg gtctgcgtca ggacgcggac tgcccgtctc
    11461 tgtttgcgaa aggagtcgag agcacgagag atttccgcat ccacctcccg ccacggagcg
    11521 ctcctgcctc gggccatcgc ctgttgcaag cgcaggctgt cgtacaagcc cagaagcgag
    11581 gcttgcccga cagcacagag tcctcctttt gagacggggt gctctgggtt tccctccagc
    11641 ttgatgggac ggccatcgcg gcacttggaa agcactccgc aagatgcgct gcatccgccg
    11701 cagacggcag cgtaatagta aggacgcccc ggcgtgatgc cctccggctg acgcagcatc
    11761 gggatcaccc gctccactgg cgcacgcccg catccggtga gtgcagctcc ggcaaatgca
    11821 aagccggcgg ctcgcaagaa gtcccggcgg cctggttcgg agagatcacg caaaggcgta
    11881 tcgacaaact cgaccgccgc ccgctcgccg gaggggccgc cttgctcgta ctcttcgagg
    11941 ctcttccaat acgtcttatt gtcggcactc acggtggcag ctcctcagta gtgacaggtg
    12001 gaacagtcga tcgaagcgaa caacttcttc ccgcccacac catttcgggt ggcttcacgg
    12061 tggcagttga cgcaccagcc catgctgagg tcgggaacct gtctcaatct ctccatcgtt
    12121 tcaacgggtc cgtggcaggt ctggcacgcc acaccggcgt tgacgtgggc gcggtggtcg
    12181 aagtacacaa aatcgggcag cttatacacg cgggtccagg cgatgggttg cggcgtgcgc
    12241 gcgggatcgg gcttccgatt ggcatcgagc gcaagggcat cgtaaatctt ccgcagctca
    12301 ggcgaaacga ttgtccgggg ttttcgattc tccttggtgg ccagctcctc ttcactgcgc
    12361 acggcgccaa acgaggcagt taccatcgtg tgacagttca tgcagaccgc ggccggagga
    12421 acgccggcgt ggcggctctt ctccgcgttg aagtggcagt aaaggcactg cacctgaagc
    12481 tcgccggcgt gcaaccggtg cgaataggcg acaggttgga ccggctcgta gtcctgctgg
    12541 tttcccggaa ggtggacagg tcgggccacc gtcaacaggt agagtgcgct gaagaacaac
    12601 ccggtagcaa ggacgatggt gaggagtcgg ctattcatca gtgcaacctg cgctcacttt
    12661 ctgttcgcgg cggcggccag cggcacggcg ccgctccgat gcggggcgga ttccggcatg
    12721 tacttcaaca cccaggccat cgacagagcg aacagagcca gaaagacaaa cacgatgaaa
    12781 ccaatcgcat aaccgcgctt gcccagatcg gccaccgcga aggccattac tggtggaatc
    12841 gcgaatccgc caaatgcgcc cagccctccc acccagcccg atgctccgcc gacagcctga
    12901 ggcacggctt gcggaacgat cttgaatacg cccgcgtttg aaacacccat gccgaacgcc
    12961 aggagcacga taccgggaat ggccagctcg tactgcgcgg cgttcaccac cacgagcgct
    13021 ccgattatcg tgatgagcag cgccagaatg gtcgtgttct cgccgccctc ccgaagccgg
    13081 tccgagagaa cgccgccgat cactcgaata agagaggtga gtatcgagaa cagggcggta
    13141 agaaaacccg ccgccaccac gctaagcccg aagaacccgg tccaatacgt tggcaaccaa
    13201 gcggtcaagg cgacgaaccc gccgaatgtc gtgaagtaga tcgaaactag agcccacgtt
    13261 ttccaagttc gtgcggatac tttgaggcta tctttcagat tgcccgctgg aaatagttcc
    13321 tgtccgtatt cgctcgcaag gcggcgcgcc tcctcgggcg ttgaacccag ctcccgcaac
    13381 tgaaagtagg cggaattcct gcctacgatg aaatacaaga ccaacccggt agcaagcagc
    13441 gccagccaca ccatgtatga gccggcaagt ccgagtgtgg tcagagcaac agggatgagg
    13501 aatgagaaca agcccggcgc cagattcccg actccgccga agatcgcgag agcgacaccc
    13561 tgccgttgtc gcggaaacca gtaggccacc tgcccaatcc cgactgaaaa tgtggcgata
    13621 ccgcatccac aaagtagggc cagaatcagc agaacgggat acatcccggg agtcatctgc
    13681 tgcggatagt agagcgagac aactgcggtc agtcccgtca ttcccgcaac gccaaggccg
    13741 agcagcgtca ggaacggttt tcgtccgccg ttaatgtcaa cccaggcaga gaaaggaatg
    13801 cggagcaacg atcccgagag cgccggcatg gcaacgagga atcccaccgc aatgggactc
    13861 aagttcatcg cctcctgaaa acgctttgcg gtaggaccga acaatgccac agcggcaaag
    13921 ccgatgaaaa agccgagagt ggctcccgac aacccctgcg ctggtgaccc tttcagagtg
    13981 cgcatcgcca ctatatcctc ctttagatgc cggccggtcc gcgataccac cggaccactt
    14041 gaggtttgcg ccacaaatat ggatttggaa tcaccaacac gtgaaccagg cgcgtgaatg
    14101 gaaacagcaa gatcagtaaa taggcgttga ctatgtgcaa cttcaccaac cacggcatgg
    14161 gagttatata accgatatcc ggattaagcc tgacaagcga ccagagatat ggcgtggcgc
    14221 tgatcgagaa ccatgacgat ccccacgggt aaaagagcgc tacggcgact cccgtaatgg
    14281 cctggagcac gagcagcgcg aacagtaacc aatccaccgg gctcgtaacg atcttgagct
    14341 tgcttacggt gaaccggcgg acgatggcgg cgctcaggcc gagtattgtc agaattccga
    14401 agatcagcgc ggcagcctcg agaatgtaaa gccggagcgg gtggctgttc cagagcaaga
    14461 ttccgcgtgg caccaggaag gctacgatgt ggcctgcgag cactaccaga atcccgtagt
    14521 ggagcggcac cagcgcccag aaatgctggc gattctcgag gaattgcgac gagagactcg
    14581 aataagtgaa cgtccgcgac cggtaacgcc ggatggtcgc cagaaagaag gtgaagaacg
    14641 cgatgtaagg caggacgttg aacaggagct gatcgagata atgggttgtg gtattcatca
    14701 gctatcccct tttgctggtc cacactctgt cccgtcgccg gcgagcagca ggattagcgc
    14761 ggacagaaga tccgcgaacg gattactctc cggtacggcc cttcgaagct tctcgagggc
    14821 gggcagcaga aatctggcgc ggaagtgcgc tgcttcttct tccggcatgc gcgccagcaa
    14881 cggtaagacg gagagaagac tgtcgggaag ttcatcacgg tcaggcacct tgtgccgccg
    14941 catttcttgg cgcagcttca caaggaactc gccgcgcgcg taatcctctc cgaagaggtg
    15001 ccagccgatt tccagacaag cgtccggatt cagatcgaac gtctgaacgt acagttcttc
    15061 catctgctga aggctgcggg cagagacggc ggtcgcgaac ttcccgagca gattcgcaac
    15121 tcgctcgtcc gtgcgcggaa gggtctccat cacctccgcg agccgttgct gatagcccgc
    15181 ggccgggtac tcgagtagct ccgcgataag gccggtggca ggggctaccg atgtcatcac
    15241 aaccccctcg tcgggccggt gaggaacccg aagccagcgc tctgctgatg ctcacgcggg
    15301 tcggccatca tctcgatggc ctcctcgcga tgcatgggcg gaatgacgaa acggtcttcg
    15361 aacgtgcaga gggacgtcag ccggtaaatg gcctcagctt cctctttcga gcagtctgct
    15421 tcgcgcagca tgcgctcaga caccgcatcc tccacgtcgc ccacggtcat ggcacgccgg
    15481 taccagcgca cggctttctg cttgcgcaga gcgtagcgga ccattccctc gtggccggca
    15541 ccgaagaggt tggccaggaa tttcatggga acgcgcgcgt catcgatgtc gtgaaacaag
    15601 tcgtcactca cattcttcac ggcgccgtcc tcgcggctcg acatcaccgg agccatgggc
    15661 ggaacgtaga acaacatggg cagtgtccgg aattcaatgt gcggcggcaa tgcgatcttc
    15721 cagactttga cgaactgata gacaggcgat ttctgcgcag actcgatgac ggagtcgtga
    15781 atgccgctgg cgcgagcggc cgcaatcacc tcgggatcgt tcgggtcgag gaccagcgac
    15841 cgctgcgcct ccaccaattc gtcgggaggg cgtgtagcca cctcctcaat ccggcttgca
    15901 tcgtaaagga gtacgcccag gtagcggatg cgtccgacgc aggagtggaa gcaggccggc
    15961 gcctgcccag tttcaagacg cggatagcag aggatgcact tctcggattt cccggtggac
    16021 cagttgaaga acgtcttttt ataaggacag gccgaaatgc agttgcgcca ggcgcggcaa
    16081 cggttctgat cgagcagcac gatgccgtcc tcgccccgtt tgtaaagcgc acccgaggga
    16141 cacgacgcga cacacgaagg attcaggcaa tggttgcaaa tgcgcggaaa gtaaaagaac
    16201 accagccgct cgacagcgaa caactgttcc ttctgctgct cggtcagccc ggcgaggttt
    16261 gaatcgtgct ccgcgtaaac cggggacccg cccaagtcgt catcccagtt cggaccggct
    16321 ttgacattga ttaactcacc cgtgactgcg gagatcggaa cagcgacagg ttggtccgga
    16381 ccttcaggcg cattaaagag gtgctggtaa tcataggtcc atggctcgta gtaatcgtcc
    16441 atggtcggca tgttgggatg atggaaaatg ttgacaacgg tctttgactt gctcgtggac
    16501 ttcagccgaa gtttgccgtc cacgcactcc cagccgcccc ggtatttctc ctggtcctcc
    16561 caggcggtgg ggaatccggt accgggcttg gtctcgacat tgttccacca catgtactcg
    16621 gcgcccttgc ggtccgtcca gatgttcttg caggcgatac tgcacgtgtg gcaaccgatg
    16681 cacttgtcca ggtggaacac catcgacact tgagatcgca cgttcatagc aggttctcct
    16741 ctaccacttc aggtcgggca ggcggcgcac gagaatgtga gtgtcgcggt tgcacccggt
    16801 ggggccccag tagttgaagt ggtacgtgaa ttggccgtaa cctccgacca tcaggttcgg
    16861 cttgagacgc acacgcgtga ggctgttgtg gccgccggca cgcctgtagt ttctgagggg
    16921 cgatttcgga accgagtatg tgcgttccgg cgaatggtac tggatgcaga tccctcgggg
    16981 gatgcgggcg ctgacggccg cacgtgtcac aaccacgccg tggtcgttgt ggacctccac
    17041 ccagtcgttg tcgacgatgc cgaggtccga cgcgtcgtgg tcattgatcc agaatggctc
    17101 gacgcctcgc gagagcgtag tcatacgctg gttgtcgccg taggtggaat gaatgtgcca
    17161 tttgccgtgc ggcgtcaggt aattcagcat caacgtcttg cctgcttcct tggtgaaacg
    17221 caggtcggag tagtctttcg ccgagggctt gggcttgaac gtgggaaggt gctcgccgaa
    17281 ctgaatgtag ccgggatgat cgaggtagat gtgctgccgt ccggtgaggg tgcgccatgg
    17341 gaccagcgct tccacgttat aggtgaatgg cgagtaagcg cgcccattct caaccagccc
    17401 cgaccacatc ggactgttca gcaggcgctg aatccgggat tgcagttcct tgtaactgat
    17461 ccggactgcg cggttcttct ccgccagatg aacgagcgga acaccagtcc gttcctccat
    17521 tccccgatac gaccggtagg ccagctcgcc gttcgtcacc gaggcgaatc gcaggatcat
    17581 gttgcacacg ctctcgtctt cgtagagcga cggatagctc ttgccatccc aatccacacg
    17641 cggcagcgtg agcagcgcct catcgtagag gtcatctacc ttgtagtgcg ttccgtgcgc
    17701 gcccaggccg ttcgcgcgca ccatcgggcc gaacgagatg aattggttgt agaggttctt
    17761 gtagtccctt ctcacgaccc gcagcccagg catcgtcttg ccgggaatcg cctcgacttc
    17821 tcccctcatc cagtcccgga tcgccggttg ggcgatttcg gcgggggtgt cgtgagcgag
    17881 cggtgtcgct acgaggtctg ctaccggctc cgggaaatgc ttcgccgcaa gctcggagaa
    17941 tttcttcgag atggtgcgga agatctgcca gtcgctcttc gcctcccagc acggcggcac
    18001 cgccgccgac agagggtgaa tgaagctgtg catgtcagtg gaattcagat ccgccttctc
    18061 ataccacgta gcggccggca ggaccagatc cgagtacagc gccgacgtgt ccatccggaa
    18121 attcagatcg acgacgaggt ccatcttgcc ctgcggcgcc tgcttatgga actcgacatc
    18181 gtgcacgaag ccttcggcca tctcctcggc gatcgcgttg tcgtgggtgc caaggtagtg
    18241 ccgaagaaaa tactcatggc ccttcgcgct cgccatcagc gcgttgcccc gccagatgaa
    18301 ccatacgcgt ggccagttct ccggcgcgtc gggttcctcc gccgagaacc gcagcttgcg
    18361 gttcttcagc tcgtccacgg cgtatctcac cacgtcgtca tccgtcttgg cgcccgccgc
    18421 ctgtgcgtcc cgcaccacct cgagcgggct gcgatcgaac tgcggataga atggcagcca
    18481 cccattgcgt accgcttgca cattcacgtc ggcggtgtgc gtgcccgtta tgccccgcat
    18541 cagcgggttt tgtggcatgg tgtggtaatc ggcgtagtcg cgctcataac gccactggtc
    18601 ggtgtgcatg tagtgccagg taggcgcgtt ctggagccgc gaggcagggt accaatcctt
    18661 gccgaacgcg atcgccgacc aggactctcc cggcgccagt ttctcctgcc cgacgtagtg
    18721 cgccaagccg ccaccgttta ctcccacgca gccgcacagc atgagggcgt tgatggccga
    18781 ccgatacatc aggttgttgt ggtaccagtg gttcacgccc gcgccaatga tcacggtgca
    18841 cttgccgccg gtgaggcgag ccgtttcgcc ccactcccgg gcgaagcgga tcagggtatc
    18901 gcggctcatg cccgtgtact tctcctgcca cgccggcgtg tagggcgcgt tctcgtcgtc
    18961 gtatccggcc ggatactcgc cgctcaggcc gcgtggcacc ccgtactgcg ccatcatcag
    19021 gtcatacacg gtcgtcactg tgacgctctt cccggccgcg gtctcgaggc gtttcgccgg
    19081 gacgccccga cgcagtgaag tcccggcgcc gaaatcatcg aaacgtacgt cgaccacctg
    19141 atcgtgctgc tccaggaagg tcaacaccgg gtcaatgccg ctccccgtca gcccgtcctt
    19201 gagcgcaagg ttccacttgc ccttctcctt cgcccagcgg tatccgctgc tgcccatcgg
    19261 catcttcggt tttgactccg cggcgtccca catcaggaac tgccagtcgc cgttctccac
    19321 cccggcgtac tccgcaagcc gattcgcgcg cagcagttgc ccagcctcga atactccctc
    19381 gcggtcgacc agttccacca ggtaggtgca atccgtgtac ttcttcacgt agtcatggaa
    19441 gtacggaact ttctgctcgt ggtggcactc cttgagaatc acgtggcccg ccgccatcca
    19501 ccacgcgccg tcctgtccgg cgtgaatggg gatgtactcg tcggcatact tggccacctg
    19561 gttaaaatcc ggggcgaaca cccacatctt tgatccgttg tggcgcgcct ctgccgcgaa
    19621 gtggcagtcc ggcgtgcggg tcatgttcag gttggaaccc gtgacggcga gaagcttggc
    19681 gttgtaccag tccgctgatt cctggacgtc ggtttgctca ccccacacct caggagaggc
    19741 cggtggcaga tcacagtacc agtcatagaa cgacagcgag actccgccca tcagttgcag
    19801 catcctcgca cccgaggcgt agctgatcat cgacatcgcg ggaatcggcg agaaccccgc
    19861 aatccggtcc gggccgtact tctgaatggt gtggatgcaa ctggccgaga tcagttcgac
    19921 cgcggtgtcc cagtccagcc gccggaagcc gcccttgccg cgggccttct gccaccgccc
    19981 gcgcttctcc gggtcgttga ccagagacct ccatgcctcg accggatcgg catgctcggc
    20041 ccgtgcgctc cgccagagat ccagcagcgc tccccgcacg taaggatgct tcacgcgcag
    20101 aggactgtag agataccagg agaaggagat gcctcgctgg cagccgcgcg gttcgtaagg
    20161 cggcagcccg ctttccaggc tggggtagtc cagcccctgc atctcccatg tgacaatccc
    20221 gtccttcacg tagatctgcc atgtgcatcc gccggtgcag ttgacgccgt gcgtgctacg
    20281 gatgatcttg tcgtgctgcc agcggttcct atagaactgc tcccaactcc gcagcttcgg
    20341 atcggtctcg tccttaatcc acgtcaatgg tccatgggtc gtcattcttc acctcgatgg
    20401 gtgctggcta gcacgagtgc cttgcgaacc gaacgaaaac gttccttcca tgcctgctcg
    20461 aacagcacga gggccagcgc cgcaccgccc aacccgatca ggaagaacgt cagcggtgcg
    20521 ggtgaggcgt cctcgccccc cttgcgcgct tcgtcctcga ggaacgcaat caatggcaga
    20581 atctcctccg gcctgagcgc gcgccgctta aagaccgaga ccatggtcgg cgtcgcagga
    20641 gccgtaagcc aagcggccag atttttgcgt ccctgcagcc gctcgaacac ccgcgtcaga
    20701 tcgggcccga gccgccctcc gctcagtccg ccgacatcac gcgcggtgtg gcacgaaata
    20761 cacggaggtc cgccgttggc gagccggttg acaccgctga acatcaggcg cccccggtcg
    20821 atctctgagg gagtgaacgg ccggtccgtg atttgcaggc ccataaactg cgatttcttg
    20881 agcttcgatt ccgcctcgat cagcgccagc agcgcttcag cccgggaggc gttcatgcca
    20941 ttgatcgtcg gcatcacgac gtccctcgcc tcttgccgca gtttcaaggc ataggggtcg
    21001 ccgctgtcca tcaacgcctg ggggttctgc acgaatcgcg ccagccattc ccgatccttg
    21061 cgtgcgagga cgcccttgag gtccggcccg gtgagccgtc ctccaccgat cgtgtgacaa
    21121 ctcgtacagt tctcgcggaa gaaatccgca gcctcctggg ccgtcagcgg cgccgcgacg
    21181 acgcacgcgg ccagccacag caaactggcc ggtgtcccga gccttcttcc cggcgaccaa
    21241 ttgtttcctg agttcatttc gacctctgct tgaatgactt cggggggcgc tgaagcgtca
    21301 cggacatacc tcgttcgccg ttccgccgga ccaggtccgc cagcgtcgtc ttctccagaa
    21361 agccgagaaa gctttccttc acccggcgcc attcctcgtg cgcgccgcag tggtccaggt
    21421 ccgagcactc ccttgtgccg ccgaggaagc accggcactc aggccgctgg cggtcaaaca
    21481 gtttcacgac ttcactcagc cggatatcag ccgccttgcg acgcaggcgg tagccgccac
    21541 cgcttcctcg cgttgcatcg atgattcccg cgttgcccag cacccacagc accttggaaa
    21601 gatagttcga gggaattccc gcagaccggg cgaggtcctt gcccagaatc accttgcctt
    21661 cttccagcga ggccatctcg ctgagcgccc tcaatgcgtg ttctgcggtt gtcgagagca
    21721 ttgtctactt cttcatgtac aagtatacaa ttagctcaga cactgtcaag agaaaattct
    21781 atttttctct ttttagctct ctcgggagct acttggggcc ggcgctccgc tttctttttg
    21841 gctgccgtct gcctggcgcg gggcccgggc gatccgcaat ctccgcaagg gtagtggact
    21901 ccagaaagcg caggatggag gtcttggtgt cccgccaagc ctgatgggcg ggacaagcat
    21961 tctcatccga acatacctgg ttgtacccga gcagacacgc tggtttaagg aaacccttgt
    22021 cgaacaaagc caccgccgcc gccagagaca tttcctccgg ccggcgggcg aggcggtatc
    22081 ctccaccagt acccctcgaa gccctgatga tccccgctcc gcctagagtg accaggatct
    22141 ttgagaggta gttctggggg attcccgtct tcttcgccaa gtccctgccg agaacagtct
    22201 cgttctcttt cagcgtggcg aggtgtgcaa gggcccgaag cgcgtattgt gacgttgcgg
    22261 acagcattct tcctcagacc atcttcccag agcgagcaaa gtatactcaa tggacgccgg
    22321 aggttggatc aaccggtttt gcggcatcgt agccgcgacc ttaccatccg gccgaaggct
    22381 cgtcagcaaa acgtgccgaa gagtgaatcc acaatgcaga gatacgtccc tttgttgctc
    22441 gttcccgcaa tcctgttttc gcaggagaac ttcggtgtgt tgcccgaagg ctccggactg
    22501 gcggccaagt atcgaggcga cagcgggatc gagcgcgacc ccgctgtcgt cttcatggag
    22561 aacttccgaa gtggaactct ggccgacctg gcagccagat ggacggatat aagcaaccgg
    22621 aacggcaagg ttttggcatt cgcggaggac gtgccgacgg gaagtgcaag ccgctggtcg
    22681 cttcgtatga ctgccacgcg caacgtcgat tcgggcggcc acctgtatcg cacttttcaa
    22741 cccggctacg accggctttt tctgcgcttc tatgtcaagt ttgcgccgga tgccggcttt
    22801 aaccaccact tcgtcagcct aggcggagag atcgatccgg caaagtacgc agtcggacgc
    22861 gctggactcc gcccgctgaa tcgttggaac actggcatcg agccggcggc ttcgagccag
    22921 cacggcgatc acaacgcgat cgctccgccc ggcgtctgga acttctatac ttactggccc
    22981 gaaatgaggt cctggcaaag cccggacggc aaagcgctcg acgataatgg aaaggcgttt
    23041 tacggcaaca gcttcgaacc caagaccccc acgcctgtac cccgcggcca atggatctcc
    23101 gtggagatca tggtgaagat gaattcttcg ccggattcct atgacggcga acaggccgtc
    23161 tggatcgatg gaaaactacg ggggcgtttc gcgcggggca caatgagagg gaaatgggac
    23221 cgggccctgt tccgcgtgga tcccttgggc gagcccttcg agggttttcg ctggagcggt
    23281 gaccttagag ccaagatcaa caagctctgg ctctcacact acgtcagcag cgagagcgcc
    23341 ttcccgcgaa cggacaagta cgccgccgag cacccgggat ttgtggtgaa cacgcagacc
    23401 cagaccgtgt ggttctcctc cgtggtggca gccacggaat acgtcgggcc tctcgggcgg
    23461 ttaccggatt gatgattgtc tgaaccgtcc tggtgagccg cgatggtgag gtattcccaa
    23521 gtcgggcaag acagccggcc gatcctgtat gctgtcccct tggccgactg aggccggtcg
    23581 cgcattcgcg caatcgtttt ggagatttcc gatgacccgg attattcttt ctttgattct
    23641 gatcgcttcc ggaacgttcg ccgctgagcg ccgggcgaag aacgtgatcc ttttcctcgg
    23701 ggacgcaggg ggtatcccga ccctccatgc ggccagcctg tacaagcaca atcatccgca
    23761 gaagttgttc ctgcagagcc tgccgtacat tgccttgtcc gacacttcgg ccgcagacgc
    23821 ctgggtgacg gattcggcgg cgggcatgtc ggcaatcgtg accgggcaga agaccaacaa
    23881 cggcgtgatt tcgcaatccg cggccgccgt tcgcggcaag caggacggcg agatgctcca
    23941 gacgatcctg gaacacgcgg agcagcgagg gctctccacc ggcgtgctaa cgaacatggt
    24001 catcacggac gcgaccgccg cggcgtgtta tgcgcactcg aacgaccggt cccgcgcggg
    24061 cgagattttc gcccaagtgc ttcacccgcg gttcggggac ggtgtagatg tcctgatcgg
    24121 cgcggggcgc gatcgcgtga tcgagtccac ggcgaagctg ggaatacaga tcgagtccgc
    24181 gcttcgcgag gagggcattg cctttcgcga ttcgctagcc gcgatttcgg accaggaccg
    24241 ccgcgtggcc gcactcttta acacgcacga tttcgacgtc tccgacgcgg tccggcgagc
    24301 cattcgcatt ctttccaaga accgcaaagg gtatttcctg atggtcgagt gggacgcgca
    24361 cacggacaac cttcgcaggg gacttgagca agtgatccaa ttggacgcag ccatcggcga
    24421 aaccgcctcg atgacagacc cggacacgct cattctgttc acggcggatc actccttcga
    24481 cctgcgcgta cgcgggggca agccgggcca gccgatcctg cccgaagagg gcgccggagc
    24541 ggcagcgaag gctcggccga cgatccgggt ggacgatggc cacacgggag agcaggtgct
    24601 ggtggcagcg cagggtccgg gggccgagcg ggtgcacggc tttttgacaa acgtggatct
    24661 gttccacatc atgatggccg cctacggctg ggacaagacc ggcgcgccta gtggcggccg
    24721 ggcggctcga tagccgccga gccctcacag tccaggccct gtgtgcgggg gcggtagtca
    24781 ccccactgca ccttcgtttt tcttcagtta gtagcggacg accttctcaa tggccctgtc
    24841 caggtcatcc accgacggaa ggaccgcgtg ttcgaggttt gccgcggatg gaacgaagga
    24901 atcctgagcc gcctctgggg tcttggcgtg aacgcgactg cgtgagctcg tggggaacaa
    24961 tcgccccggt tgaggtgaat cagatcgccc tgcatgttac gttccgcgtg atcggagtca
    25021 ctgaagcgaa gagcaagccg ctctaggatg agttcaggag gcagaaatgc agggaattca
    25081 ggatgctaca gtttcggaga ttgcggccag ttcgctggcc gcggtgcgcg ttttcgagcg
    25141 gttgggaatc gattactgtt gtggcggcaa gcgcccgctg gcggatgtgt gccgggagac
    25201 agggcgcgac cttccttcgg tcctgagcga tctcaagtcg gcaatgagcg cgacccaggg
    25261 tccggctcgc gattgggcca cggcgctgct gcgagagctt atcggacaca tcgtcggcac
    25321 ccatcacgag tacttgaagc gcgaactagc cccgatcggc gcacgtcttg acaaggtcta
    25381 ccgggtctac aacgagagat acggtcccac acttaccgcg cttccagagg tcttcaacgc
    25441 gctacggtct gaactggaga tgcacatgcg caaagaagag atggtcctgt ttcccgcgat
    25501 cgcctcctat gagtcggctg ctgtcgccgg ttcacctctg ccgggaacgc cctttggcac
    25561 cgtcagtaat ccgattcgaa tgatggaagc agagcacgac agcgcgggaa cagcactcgc
    25621 ggacatccgc gccataacag cgagctacgc aatccccgag tatgcctgcg ttacgtaccg
    25681 gtccttgatg gcgggactcc aggaactgga gcaggacttg cacatgcaca tccaccttga
    25741 aaacaacatc ctgtttccgc gcgcgatcgc aatggaaaac gccgggcgtt gatcgcgctt
    25801 tgtgcccgga aggcgcgtac cacgctgcca gggagttggg accggaaacc aggcccatca
    25861 gcgacgccgg ccgtggtgta tgctcatacc atggcgacgc gttcacaatg atgaatcgcg
    25921 ttgaaaagta agggggagac cgtgatgcgc ctgacgagtc tattgctgac gaccattctt
    25981 atggtcctgg ttctgtcttc ttgcaccgac aagagtgcgg tagagctttc ggagagaagg
    26041 cacgccacag tcttcatgca ggacggcacg aaggtggctg gcaccgttgt ggccagctcg
    26101 gcgtcggaga tcactcttgc cggcgacgat aacatcaccc gaaccatccc gatgaagcag
    26161 gtgaggtcgg tcgagtacag tgacgaaccg gttgcagaat cgcaagcgcc ggccaagcgc
    26221 gagccggaac cggcttcgcc cgcgagaaga tcggcgcccg cgcccggccg ccagagcgca
    26281 gctccagcgg cgcaatccgc gccccgatcc agaacttacg agctcgccgc tggaactgac
    26341 attccggtgc gaaccaatga gacgatcgat tccagcacag cggctgaggg ccagagttac
    26401 gccgccgagg tgtcacaaga cgtgctggac gcttccggta cggtggccat accgcgcgga
    26461 tcgcaggccc agttggttat cgtgtccgcg tcgagaggcg gccgtattcg gggcgcttcc
    26521 gatcttgtgc tgagccttgg gtctgtctct gtgggaggac ggaactacgc accggagacc
    26581 gccagcgtgc agcaaagcgg caaagcgggg atcggtgcaa ataagcgtac ggccacgtac
    26641 accggcgggg gcgccgcggt gggtgcgatc atcggagcca ttgccggcgg cggtaaggga
    26701 gcagcgatcg gagcaggcgc cggagccgga gcgggagcgc tcacgcagat cctgaccaag
    26761 ggcaaggcca tccgtgtgcc ggccgaaagc gtcctgacct tcaagcttga ccagtcgctt
    26821 aggatccagg cggaataata caccatggca agccggctga gcagagcaag cgacgtgctg
    26881 cactctctcg ggctgttatt tctggccatc attccggcta taggcttcgc tcagaaccca
    26941 ttcagcctca gcgagaagga cgagatcgag ctgggccgtc tggccgccgg ggaaaccgag
    27001 aagagcgtcc tcctcatcaa cgatccagcc gtcgtcaagt acatcgacga tctcggacaa
    27061 tctttggtcc agaagtcgac tcgaaaagag atcgcttata ccttcaaagt cgttgatagc
    27121 ccggagatca atgcgttcgc gctcccggga ggcttcatct acgtcaaccg cggattgatc
    27181 gaggcggcgg agacgcagga cgaattggcc ggcgtcctgg cacacgaaat cgggcacgtc
    27241 gttgcgcgcc atggagcgga ccaagctgtg cgcaccggac tagtccagac tgggatgggt
    27301 gttttcggtg gtctgctggg gcgcggaacg tcctcgtctc tgggcaagat cgctgccgag
    27361 atggtcgcta ccggcgtctt catgaagttc agccgccagg ccgagcgcca ggctgaccgc
    27421 ctcggcgctc gcatggtcta cgacgccagt cttcagccgc agggcatggt gagtttcttt
    27481 aacaagcttg ccggtctgca gaagagccag cccaacgcag tgctgaagtt cttttcatcg
    27541 cacccaagcc ccgccgagcg ctccagcaac gtctccaact tgattgccag ctatcccgcc
    27601 agttccaaac cggacggcga tagtccggtt ttccgcgatg tgaagcaacg tctggcggcc
    27661 ctgcctcgcc ccgaggttga agcggcaaag gcggcggctg ccattgccgc cgctgcccaa
    27721 ccgcccgccg agccggaaac ggacgttcgt tctcgaaaca tggtccgcga ccgcgatgtc
    27781 gctgcccgct tcgcgcccgt gtttcaccaa gccctgggac ccgcgccgcg ttttgactac
    27841 atcactaatt tcgacttcga cggcgactgg cgcggcgata acaactggaa taatgccgag
    27901 aaccgcagct accccttgaa ggcatacgtc tactatgccg tctttgagac ccgcactcac
    27961 ttctttattc actacgctgc tttccaccct cgcgactaca agggcggcga gaagcgcggc
    28021 gctttgctga gcgaggtgat gcgtatcgga gtgggtctcg gcggccagta cgatcccaca
    28081 ggccggtcga acgaggcagt gatggcccac gagaatgacc tcgagggagc cctcgtcgtc
    28141 gccgagaagg atggcgagga ctataccaaa gcgcagctta ctctcgtcga aacgctggcc
    28201 cacaacaatt acctcaagta cacgccgcct ggaagttcgc ttcccggcga tcccatcatt
    28261 gtggtgggtg tccgaccccg cttatttgtc gaatccaaag ggcacgggat tgaggcctgg
    28321 cgggacgatg ccgaccagca caagatggcg gagaatggct ttctggttta tgtcttcacc
    28381 gccgcggccg aggatccgga cgagaaaccc gaccgggcgg tagggtatga gctattgccg
    28441 acttacgaaa gcctttggcc ccacgcacgc tctggcaaga acgaaacctg cggtgaggag
    28501 catgactacg gcgttgtctc cgccaagttc gcccaatgca acgaagacgt cttgcgccac
    28561 atccggctcg gcgtgatggg ttcggccttg aacggtgtgg tgggagcggc taacatgtcc
    28621 cggccgccct ggggctggtt cgatggacag gatcgagccc ggccgcttgg tgagtggttt
    28681 ctgcagcccg ccgaaacgat ccgccgccgc tggaatctgc cggaatcgtt ctcgaccacg
    28741 tatacctacc atcccttcct cggccagttt cgttgaggac cgggccgcga gcgcaaaccg
    28801 cggttgagct tcgtgccgga cagaccaacc cgaaaccgga acacccgcga tctacgagtt
    28861 atcaaacttt aggaaatctt caccgccgac cgggcactct tcacgccgcc gactgtcagg
    28921 gagcgggcag tatttacact ttccctaggg tctgacatag gagctttggc cgtactgtgg
    28981 aaagtagagg ctccaataac catgcaacat tcgaagcgtt tgtgcgtctt gctgatcctc
    29041 gcgggtgcgc tggctctggc gccggctgcg ctcgcgaaac agggcttcgt gcaacacaat
    29101 ctcgtatcgg acgtcgaagg tctggctgac cggacggatc cgaatctcgt taatccatgg
    29161 ggtatcgtac acagcgcgac gagtccctgg tggatcaatg cgaacggaac agggctgtcc
    29221 gttctttacg acggcaacgg cgcggcgttc ccggcggcga gtccgttgat tgtgacgatc
    29281 cccgggctgg ttggcacatc cgcaccgacc gggatcgtgt tcaacgggac cgcggatttc
    29341 gaggtagcga ccggcctgcc ggctagattc attttcgcga ccgaggatgg gacgatttcc
    29401 ggctggaatc ccacggcgga tccgacccac gccattctaa aggccaatcc gcctgacgcg
    29461 ttgtacaagg gtctcgcgct cggaagcatg ggcactcaga acgtgctttt cgcggcgaat
    29521 tttcgcaacg ggaccgtcga tgtcttcgac acgaacttca atctcgtgtc catgcccgcc
    29581 ggggcgttcg tggataagaa tgttccgcaa ggtttcgcgc cgttcaatgt ggtgaacatc
    29641 agcggcgtca tttttgtcac attcgcaatg caggatgaag aacacgaaga cgatgtcgcg
    29701 ggtccgggcc tgggttatgt cgatgcgttt tcgccagacg gaaccctgct catgcggctt
    29761 gagcatgggc gctggatgaa cgggccatgg gcggtggtat tggcgccgca gggtttcggg
    29821 aagctcagtg gccgcctgct ggtgggcaac ttcggcagcg gacagattgc gtccttcgat
    29881 ccggaaagcg gcgaattcga agggatgctg cgcggcctgc ggggcaagcc tgtcacgatt
    29941 gaagggcttt ggggactcgg cttcgggaat ggcgccaacg ccggatccgc cacgacactc
    30001 ttctttgcag ccggaatcga agacgaggcg cacgggctgt tcggcaccct gaccgcggtg
    30061 aagaacaaca acgaggatga tcagggagaa gacgataatc aatgaggatc gctgaaggtg
    30121 gatagtttcg accacggcgg gcctcgctta gcgcgagcgt ccgccgcggc agagagagtg
    30181 atgatttacg cgccggggct ttggtggctg gcaacgccga atcgcagata gagcgccggc
    30241 atgagcagca ggttcattaa cgtggaagtg agcagtccgc ccacgatgac cacggccatg
    30301 ggatactcga tttcctgacc cggcgtgctg ccggccagta caatcggcag cagcgcgaga
    30361 gacgtcgcca gagcggtcac aaagatcgga gccagccgct tgagctcact ggcggcgacg
    30421 tgacggcgac ctctggccgg acttgacgcc cgctccgcga tcgctcctga aaccgctttg
    30481 tgatcctagg ccgcggctca gagagtcacg acaaacagaa cagtcagcgc ctttctcata
    30541 acaggttcgg aatgcggtga ttctatactc ctgcgcggtg gcccagcgaa cgcggcccgg
    30601 gaagcctctc tgcggttttc ccggacgtgg atatgagcct gaattcggca ctctatttct
    30661 gtttagcgat gatctggttc ttgatcttgt cgtagacgtg ctgaggcaga atattcttct
    30721 ggacgagctc gtttttggcc ttgtaggggc ggccgccgat gatcttcgcc gagtaggcat
    30781 cgccgattcc ggggatcgct ttgagctggt cggccgtggc ggtgttgatg tcgatgagat
    30841 cggcggcttt tttcgtcgcg acctgctgcg cgacggccgc gcccttcttg acctcgccct
    30901 ttttcggggc ctgtgctgcg caagccaagg tgaacgcgag ggccaccgcg agtgcaatca
    30961 gaattcgctt catggtttcg tcatcctccc tcagggaagt cccgttccgg acttcgggct
    31021 tgaaatgagt atactcccgc gacacgcccg atgggctgag aaatccgggt acttgcaaga
    31081 tgtgctacag tttcggccat ggctgggaag tctattctga tcgtcgatga tgaccccgcc
    31141 atccacgttc ttctccgcca actgttgaat gcggaggatt ggcaggtcga tagcgccata
    31201 gatggcgatg aagcactctc acgactgctc gccggcgcct atgatctggt actggcggat
    31261 atcgtgatgc cgggcatgga cggacttgcg ctgctgaagc gaatccgcga ggtgcggccc
    31321 ttggtgaagg tggtcatcat gaccgctgtg aacacacccg cacacgtggt ccagtcaatt
    31381 cgcaaccaag cctactcgta cctgggcaag cctttctctc gcgaggctgt ctcagaaaca
    31441 ctccacgccg ctttggtgga tgcggtgggc gaggacgata ttgtggtcca ttcggctcag
    31501 ccaaattggg tgtcagtgga actgcggtgc aagctctcga cagccgagcg gctaacgcag
    31561 ttctttcgcg aacttagtgc cgaccttaag accgaagatc gggagatgat ctcgacagcg
    31621 tttcgggaac tgctcatgaa tgcgatcgaa cacggcggcc gttgcgatcc cggccagaag
    31681 gtcaacctga ctttcgtgcg cggcgccagg tgcatcttat attacatccg cgaccccggc
    31741 gaaggattct cgttcgagag gctgccgcat gcggcggttt ccaattctcc cgagcggcct
    31801 ttggcgcacg ccgaggttcg gggcgcgctc gggatcaggc ctgggggatt cggcatcctc
    31861 ctgagctgga acttcgccga cgaggtcgtg tacagcgaaa ggggtaatga ggtggttctg
    31921 atcaagtatc tgaacccatg aatgtggccg gcgacccttt gcatcggtgc ggaatgaacg
    31981 gctatacttc gttaaaccat agtggttggc tgggggccgt aacgatcaat ggcttctttt
    32041 cgcacagtcg agccaagggt ctgaaccaga cgtggaggac aatgtgaaaa gagcagcgaa
    32101 gccatctatc atcagatcaa aggcaggaaa atcgatgaac accgccgaag gaaacaacgc
    32161 caaacgcagc gccgccgctt cggaaccagc acccgacagc ggtaagtcac gctctcccgt
    32221 gtccgagcag gcgcctggaa acgtcgataa gatccgggac atactcttcg ggtctcagat
    32281 gcgcgactat gaggttcgct tcgcccgcct cgaggagtct ctccgcaagg aatcgtctga
    32341 ccttagggag ggcactaaga gacggctgga caccctggaa ggctacatca agaaagagtt
    32401 ggagtcgctc gaatcgcgcc tcaagagcga gaaacaagag cgcgcggaat cgcatggtgc
    32461 agtttcggcc gacctcaaga gcacggccgc ttcgctgacc aagaagatca gcgaagtcga
    32521 gaaccagaat ggccaagccc agcgtgaact acgcaaagag cttttggagc agtccaataa
    32581 gctcagcgac gagattcggg tgaagaacga agaactggca tcgcttctgg accggcgcgt
    32641 gcaagaactg cggaacgaca agacggacag gactgccctg gcgaatctct tgaccgaggt
    32701 agccatgcgc ctgaccgacg agttccatgt acccggagcg gaataggacg ccatccagtt
    32761 cgacagcatg tccagcgaag ggtcagccat tcatcagggc tcagccgatg acgaattcgc
    32821 ggaactgagc gcgattttgc tcgccgcgga gcggagccag ctttcggaac tgaagcaccg
    32881 gctggaggat ccggaagcgc gggcgcaaga cgtggccctg gtgctcgcgg aagccatccg
    32941 gttgcgcgcc gcaggggata cgaaactgcg gcgagcgcta cagcccacca ttgaagaagc
    33001 gatcggcgtc gccgtcagaa aaaacccgca catgctggcg agcgcgctct tcccgatatt
    33061 cggcaaggcg attcgcaagg cgatcgcttc cgaattggac gggatgatac aggcgctgag
    33121 ccagattttg gagcagagcc tgtcgctccg cagtctgaaa tggcggttcg aggctctgcg
    33181 cacgaacaag tcctacgccg aaatcgttct tctgcgcagc gttctctacc gggtggagca
    33241 ggtcttcctg atccaccgaa aaaccgggtt gctgctgctg catgcgcaaa gcggttcctc
    33301 cggactcaag gacccggaga tgatctccgg catgttgacg gccctccaag acttcgcacg
    33361 cgacgccatt agtggcgctg aaggcgagaa tctggagacg gttcgcgtgg gcgacttcac
    33421 ggtggtcctg gcatacggcc ccgaactcat cctggccggg ttcgttcgcg gcgtggcccc
    33481 gagggggctt aaccggctgt ttcaggacga actcgattcc atacaggagg agcaatccgc
    33541 tgcctcggtg gacttctcgg gagatacgtc gaaattcgag agcagccggc cccatctcga
    33601 gaagtgtttg gtggggcagg gcgacccgcg ggccaaagcc ccagccagtt cgctggcagc
    33661 gcgcctcctg ctgttcggta ttccggcgct tctgatcgcc gtggcgctgg gctggtggct
    33721 ggtgtcgcta cgggctgaac ggcgctggca gagcctggtg gaacggatta gctcgcaacc
    33781 cggaatcgtg atcacgacct caggcgagca cggcggcaaa cgcttcatct ccggattgcg
    33841 cgaccctttg gctgccgatc cggcggcaat ggtcagcgcc gcgggtctct ccagcagtca
    33901 agtagagttc cggtgggagc cataccattc gctgcatccg cgcttcgccg cggagcgcca
    33961 atactccgcg gtcaaagaac agttggaggg gagcgcgatt cggtttagtc taggcagcgc
    34021 ggccatctcc gccgaagcag ggcatgccgt ggatgaactc gcatctaagg tcacagcttt
    34081 gttcaatgcg gccgcaacta ccggacggga ggtcaagatt gagatcttgg gcagcaccga
    34141 tttccggggg agaagtgagc tgaacgtaaa cctggcacga gatcgtgctg ccacggtgga
    34201 ggcagcgctg gcaggcttgg gggtccccag ggaacggatg gaggccagag gcctgacttc
    34261 accgtgcggg ctagcgcgcg gccgtgacga cgccgcatgc aggagtgcga ctctccgggt
    34321 gctcgaggtc agtcgttagc ccatgatcaa gaaaaagatc tgcatggtcg ggtcgttcgg
    34381 cgttggtaag accagcctgg tgaagcggtt tgtggaaagc atcttcggcg aacaatacct
    34441 gacaaccgtg ggtgtcaaga tcgacaagaa ggatgtggtc gtcgacggct gtacagtgac
    34501 gctggctatt tgggacatcg ctggagaaga cgatctagct gagtttcgga tctccaatgt
    34561 tcgaggcgca tccggctatg ttctcgtggt tgatggatgc cgccgagcca gtctcgaaaa
    34621 agcagtcgat cttcaaaggc gcatcactgc ctccttcggc cttttacctt ttgtattggt
    34681 cgccaataag gcggacttgc gcgacgagtg ggagattcag ccggatgcaa tcgcggcgct
    34741 catcggcgag ggctggcacg ccatcgaagc cagtgcgaag acgggtgacg cagtcgagca
    34801 ggttttccat acgctggcga aagacatgct tgatcgcgac gcttcccgcg tggtcgaggg
    34861 cgacgaggag tagactatcc cgatgctgga cggcccgcgt actttccggg cgggaatgga
    34921 cctcctgccc ttactcggtt tcgcagtttt cgaaagagcg gaagcaggtt cgtttcgccc
    34981 ggccggccag ccaccggaat ggttgcgaac gtttccggcg gaagcacaga ccgtttgcac
    35041 caccgatgct ctggtcgaga tcttcccgta tctggatgtg ttcttacccg acgcggaaga
    35101 gcattggaac gcaggcaaac ccgcccggct ccattccgag atgtggactc agaccgacct
    35161 cgagggcaaa gagtggcgcc tacgcgcaac cgccacttgc cttgaagctc gcgcgttgct
    35221 ggtgatcgag cccgccgagg tccttttcaa cgagacgcag agtttcgtcc agcatgctca
    35281 cgacgccacg ctggcatatg acaaagtggc gaaactgagc cgcgcgctga aatcggcgaa
    35341 tgagcagttg gagatcagga atcgggatgt ggaacgcgcc accaatgcga aaagtgagtt
    35401 tctcgcccgg atgagccacg agatccgcac gccgatgaat gccatactgg gcatggccga
    35461 cctgctttgg gaaacccctc tcagccccga acagcgggag tacgtccgcg ttttccggcg
    35521 cgccggggac aaccttctga acctgatcaa cgacattctg gacctctcca aagtggaggc
    35581 cggccagttg gagttggagc aggccgattt cgacttgagc gaagtcctcg agaaggccgc
    35641 tgagatcgcc gcggttcgtg cacacgccaa ggggctcgaa ttgagttgtc gcattaaggc
    35701 ggacgtgccg catctgctaa ccggagacgc cggacgtctg cgacaggtca ttctgaactt
    35761 gctgggaaac tccatcaaat tcaccgaacg tggcgagttg acggtttggg tcgaacttga
    35821 cccggaccgc tgtgagcccg gcgctttgcg tttccgtgtt gaggacacgg gaatcggaat
    35881 cgcacccgat aagctggcaa ccgtcttcga gaacttcacc caggcggaca gttccatcac
    35941 gcgcaggtac ggcgggacgg gactgggcct ctcaatctca aagcggtttg tggagttgat
    36001 gggtgggcgc atgtgggtag aaagcagcct gggcatcggg agcgttttct atttcacaac
    36061 tgtcttcaag gttaggaacg ttcagccgtc gcctccacca gagttcaccg gactccgctg
    36121 cctcgtggtt gacgacaatg cgaaccatcg ggccgcggtg tgcgacatcc tgacctcctg
    36181 gggcgccgtt gtcggtcagt cggatggccc tgatgcggta gcaagactgc gcgccggaat
    36241 gcagtccggc gcaccctgct cgctggtgct cctggatggg gtgatccctg gcatcgacag
    36301 tttccacctc gctaaggtta tccgcgactc gatgctctcg agtcaggttc tgctgatgct
    36361 gacaaccgac cgtccggcgg acgccgcccg atgccggcag ttgggtatcc ggacgctgct
    36421 caagccggta cgccggtctg agctgatcga cgtgtggcgt accggcgcac ctttgcaacc
    36481 tgtaaccaca cccgatagtg tgccgacggt gccggagttc gactcgatgc gtattctcct
    36541 ggcggacgac tccgatgata accggttcct gatccggtcc tacctgcgga actcgggatg
    36601 cctcttggat gaagccgaag atgggcttgc tgccgtccag aagttcagag cggcaaagta
    36661 ccaactcgta ctcaccgatg tagaaatgcc tgtcctggac gggtactccg ccacccggca
    36721 gatgcgggaa tgggaacggg agagcggaag gcgaccaacg ccgatcctgg cccttaccgc
    36781 acatgcactc acggcagaag cacagaagag cgtggatgca ggatgcaatg cccacctcac
    36841 gaaaccaatc caaaaaagcg ttctcttaga ggccatccgc cagtacgctc tgcgggaagc
    36901 atcagaaacg atccgagcgg tggtggactc ttcgttgcgg gaaatcgtgc ctggatatct
    36961 tacgaatcgc cgcatggacg tgatcgcttt tcggaccgct ctcgagcatg acgattttca
    37021 gacaattcga atgacggcgc acaagctcaa ggggaccggc ggcggatatg gttttccaat
    37081 gctgacacag ctaggggcgg caattgagaa tgccgcgatc acacgagacg ccgagtctat
    37141 ccgcaagaat atcgccgaac tggaccgata tctggacaag gttgaagtgg agtaccactg
    37201 acagcgaggt agcacggaat gaaagttcgc ttttggggaa cacggggatc aatcgcgacg
    37261 cctggcatcg gaaccaccag gtttggcggg aatacgtcct gtgtggaagc cgttacggat
    37321 tccggcgtcc gtttcgtcct cgattgcggc accggcgcgc gtttgctcgg ctcgcacctg
    37381 gccgctagtg cgccgagacc catcggcgcg aacatcctgc taagccatac tcactgggac
    37441 cacatccagg gattcccgtt cttcgtccct gtattcgtac ccggaagccg gattgcggtg
    37501 tacgctcctc aaggtggcgg ccgcagctta tcggcagtac tggccggaca aatggagttc
    37561 acctatttcc cggtggaact cgaccagctt tctgcggaaa tcacttacca cgacctcgcg
    37621 gaaggtactc acgtcatcga tggggtcacg gttaaggcgc agtatctgaa tcatcccgcg
    37681 atgacgctcg gttacagaat cgaagctgac ggagtttcta tcgcgtacct gtgcgatcac
    37741 gagccattct ccaccacttt gtggcaaacg gattcggccc ccggaaagat caaatctatc
    37801 ttgcacgatg gcgaccgccg tcacgccgag tatatgtccg gcgccgacct ggtcatccac
    37861 gatgcgcagt acacccctga agagtatccg gccaagaaga actgggggca cagcacttac
    37921 gagtatgtag tggaagtggc cgcagccgcg ggagtagaaa gtctggttct gacccatcat
    37981 gaccccgccc acgacgatgt tttcatcgag cgtatccagg atctggccgc cagtgttgcc
    38041 accgcgcgcg gctcatccat gcgagtctct tgtgcatacg agggttgtga ggtagtcctc
    38101 aggcgtggtg aaagccggga ggcggcagtc gtccagcccg gtagtgctcc ttcggcggag
    38161 ggcggccgga ttttgatcgt cgatgatgac ccggagttgc gcgcactggt tcgggtaacc
    38221 ctggtgaagg gaggctttac ggtatcggaa gcagcaagcg ccgccgaggc acttcagatg
    38281 gtcggtgaga atccgcttgt cctcatcatg ttggatgtgc tgatgccgga aatggatggc
    38341 ctagaattac tccgcaggct tcgtgaactt ccggcggcca gaaacctccc ggtattgatg
    38401 ctaacttcac tgaacgacgc agagtccatc cgcaaggggt ttgacttggg ctgtacggat
    38461 tacctgagca agcctttctc gatgccacaa ctgacttctc gcgttcgagc ctgcctcgcg
    38521 cgggcggccg gagtagcctg agatttcatc ggatctcacg tcacggctgt tgaaaccagg
    38581 ccgcggcgaa ctcagtttct tcgggccgta gccagaatga cttcccacat ctcctggcga
    38641 aatcgctccg cggtgaatcg ttggcgccag aggcggcgcg cgtttccggc agcaagcgca
    38701 aggcgctccg gcatggagag aagttcacgc aggcgccgcg cgatgccgga cggcgatgcg
    38761 gaatgcaaca gaaagccgtt ttcgccatcg cgaatggtct ccgcaagatc gccgactggc
    38821 gaagctagga caggcacacc ggccgagaag gcgtcgagga gcacataggg gaccccccct
    38881 tcacatctcg atggcagcac gaggagatcg agcttcgaga gcacttcgta cacgtcgtcg
    38941 gaccaaccga gatacttcag agaagcgggc gccgatgcca ggacttcccg cttgtaccgc
    39001 aaagcggcct catctccttg gcgtgcgtcc ccgcagagaa ggaactccgc gtcgggcatc
    39061 tcgggcgtca gttcggcggc ggccagaacg aattccttct ggcgcttatc cggctcgatg
    39121 cggccgatca tgccgatgcg cgggcccgat gctctcgcgg cgcgtctcca gtccggcggc
    39181 ggcccgtcga ctccgccgta aatgacacgc gcacgagcac cccactgatg agcgacgaac
    39241 cgcgttgcgg cgaggaccgt ggcacgagtg gcattcagcg ccgcggcaac cagcggcttg
    39301 ccattccata tcgagacgta attgtgcgaa tgaaagatga ccgggcggcc gagtcccgct
    39361 cgccagacag caggcatcag acgcggtcca ttgacgtaga ggacagcggg ctccagaagc
    39421 ttgtcgagct tgcggatctc ggcgggaaga actgcaaggt cgcggaggaa acgcgcggcg
    39481 tcgctcaagt gcttggggcc gtcggaatac tggctgagtg gaagcacgtg gatgagcgcc
    39541 tcctgttctc ggagacgcgc agcaagcggg ccgtcatcgg gcaagcccac atgtgcttcc
    39601 cagttcgcgc ggcgcactgc cggtaacagt tcaagcaaac tgcgctgcgc tccgccgagg
    39661 ctggaaaact gttcaagaaa cagaattcgc attttcggcc tcacgaacgc cgacggccag
    39721 cgcggcgaca atcgcagcca ggactcgcca gtaggtcaga acatctccgc tcgacatctg
    39781 gaccgcgaag ccgcaccaga aggcgaacag ccatagccca aacagccgcc gcatgtcgtt
    39841 ctgtcccgag cgctgggaag atttcaccca tcgatacgtc gagactagga cggcgaagtg
    39901 gagagccagc agggagacaa gaccgagtac gccggtctcg atgaggaggc tcaagtacat
    39961 gttgtcggca acgaccgggc ggcccaggta tccggtgtac ggcagcgttt tgtaaccgat
    40021 gccgacgacg gcctgccagg ggtgctcgtg gaggtagcta atgaggaagc cccagctctc
    40081 caggcgccgc gagaggatca ggttcggcga ctccagcaga tacactccgc tgtaccagaa
    40141 gcgctccaca taagctgtga ggaacgccgg tagaaacgcg tagagggcag ccccgcccgc
    40201 gaggatgccc gcgagcgcga gaaagaccag gcgaatcacc gtccgaacga tccgaaggtt
    40261 ttcgcgttca agccacagga gcgcaccgag cgagacgaca aggctaacca tcgccgcacg
    40321 ggagaaagac agcaccagcg cggcgaggat gacagtaccg cccgtgagca gccacgccga
    40381 ggtgagccgc agtgctgggc cgagtttcag cacggcgacg gcggcgagca tcaccagtag
    40441 gaatacacag aacacaccaa gcgtgctcgc ctcataaaac acgccctgtg cgcggcggaa
    40501 cacaccacct ggaagccaga cgaactgctc ggcgaatcgc accggagcgg gaaactggaa
    40561 atagaagtca aggcaggcaa agatcgcgcc gaaggctccg gtatgaaaca ggaggcggac
    40621 gagccactcg ggaggcaatt cgcgaccagg cccatccgca agatagaaga atgagtacac
    40681 ggagatgagg aaaagacaga tgcgcgccgc ggactcgaac gccagccgcc agcccgagta
    40741 gatcgccgcc atcgggatgc tgagcaggag cacggcagtc aaagacacta atgcaagagc
    40801 gatagaccgc cgccgcagcg tccaggcacc gaggcgtgcc aagccggccc acactccgat
    40861 ggccgccgcg aacagagaag ggtgtggccc tgcgttgccc agagccagag ggagcggtgg
    40921 gagcaggagg gccatgccga ggaacaccat cacccagcgc gaggcgccgt tcagcatcca
    40981 ccacgcggcc gcgacgagga cggcggcaat tgtgaaggcc agtacgttcc tcagaccgga
    41041 ggccgccgag agcgccacag cggtcaatcc agccccgcca cctagaagga gaccgagtgc
    41101 gagctcgcgg cgagcgcttc tctcgctcat gaagccctct tgggctcgat tcccagccgc
    41161 cacaagcaga ccgccgcgac ggctgctgcc gtgctgaagc cggcgaccac cggcagtcct
    41221 atccattggg cagaggagat catgccagaa agcgatccgg cggcattgat caacaatgcg
    41281 agccctgcgc agatactcgg gatgcgccat tcacccaggg aaaacatcga gcgtgagatc
    41341 acatcgagtg cggcccaggc gacaagcacg ggacagaagc cggtcaagat atccgtcacc
    41401 gtccgggtgg aagcggcgct gaagcggccg cgctcgaata gcaccgagac aaccaacggc
    41461 gcgagcgcaa tcatcaaaag cacgacggcc agggccgcga gcgcgctgag gatcgcggcc
    41521 cgaccgatca ctccgacggc gaggcggcgg ttctttcgaa gccggaacgg cgccagttca
    41581 ctcatcagag acgatgagag cgaattaacc agcagtgcca aagggacacc ggtgagcttg
    41641 aggcagtact cgaacgcaac cgtagaaccg gaaccgaagg tcgaggccag cgcgcgtgtc
    41701 accaccgggc tcagtccgat cagcagagag tacgaaagca cgggtccggg accaagaatt
    41761 aactcgcgta agcccatacg ccggtgcgct gctgttcggc cgcgcagaat cggccaagag
    41821 atcgacgtcg cagcggcgag ttggagccac gcgccggcgg cgtacccggc ggcgaatcct
    41881 gccgccccga tccatgaatg caacagcagc gcgccgacga cggtcgaaac gttcaccatt
    41941 ccctgatgaa acgcggcgaa cgcgaaccgg cgcttgctgt aaagaagtgc ggtgaacagc
    42001 gtgctcaggg agagggccgg gattgcagtc gcggtgaccc tcaatagttg cgcggcctga
    42061 gaaatggtct ccgcggacag gcctggcgca agaaggcgga ccagtggttc cgcggcaaag
    42121 atgaacagca cggacacagc aaccgccagg cggaagatcg gccgagcgag atcggagaac
    42181 gcgggcgttt gttcgggcag cgccagcaga ggcccgaaac cgaagaccac cgtggtggcc
    42241 aacacggccc accacaggtc gacgggcgcg aaagcaaccg agagcgcatc ggccggcggg
    42301 ccggttccca gcaggtacgc gattaccacc tggcggacga agccaagcaa ctgcccgccg
    42361 aagatcgctg cgccggccac gaggccgccc ctgacgacct tccactcaga gatccgcgct
    42421 gaggaggtcc cgaaagcagt gctcattcga ggataggacg aaaggaacct gcgcccgaaa
    42481 gaacgcattt acactgtccc ggaatctgtg ccgggaaaac aagaggctct gtagacgaat
    42541 tgcgcgagaa tcccattcca tcgtctccat tttctcgacc gccgcaagga gagatccggc
    42601 tgtctgtggg cgaaaatgca ctccggtgcg gccgtcgacg accgtttcgg tggctccgcc
    42661 ttcgccgaaa cagactacgg ggcggccgca tgcctgggcc tcgacaggaa ccatgccgaa
    42721 atcctcgcgg cccggaaaga cgagcgcgcg gcagttgcgg tagagatcgc gaaggcgctc
    42781 gtcggagacc tcgcccagaa actcgacagg cggccgtgcg cgcgcgcgta gatccttccc
    42841 gagcgtgcct ttgccggcca caagaagacg acgaggtccg ccggagaagg cgtccaccgc
    42901 aaggtcaatg cgtttgtagg gttcgaggga agagacgacg aggtagtaat cgccggccgg
    42961 ccgcatgtcc ggcgagggcg taaagaagtc ggtgtccacc ggaggataga cgaccgtcgc
    43021 tcccgctcca tagatgctcg agattcgcgc gtggacggtt tcggaatttg cgagaaacca
    43081 gtcgacgctc agggctgtgc gccggtcgaa acgacgcagg tagggagcga cgagggcaag
    43141 cgcggcgcgc ttccacagcc gaccactgcc gaaacggaaa tagtcctctc tgttgtccca
    43201 cacatagcgc atgggcgtgt ggatgtagct gatgtgcgtt gcgccggccg gcacgcatac
    43261 gcctttcgcc accgcgtggc tgctcgaaat cacgagatca tagccgctta gatcaagcga
    43321 gcgggcagca attggaaaca acggcagata gtaccgatac gcctgccggg ccagtggcag
    43381 tttgtcgagg agcgacacgc cccggactcg cctctcaatc tcagcggtca cgttcccgcg
    43441 tttccagaca agcgtgaaga catccgcatc cggcaggagg cggatgaatt cggcgagcac
    43501 tttctcgcca ccgcgcatgc cagtgagcca gtcgtgaacg agagcgattt tcaagttctc
    43561 ggcgccaccc agcgccgttc accaacgggc tccaccgggg cggaccggag gccaaattcg
    43621 atggtcaagt agagcagggc gagaagcagc ccgagcgcgc ctgccacgaa cagattgaga
    43681 gggatattgg gggaggacgg ccgttccggc ggcacgccag gatccagaat gttgagcctt
    43741 tcgccgcgaa gacctgatgc ggactgcaat tcgagcaggc gcttctgggc ttgctcacgg
    43801 acggcacgcg cgtcgtcgaa ctcggtctct gaggcttgca gttccgcctc gcgactggca
    43861 aggctcttct gcatgtcggc gctcttgcgc tccagatttg cctcttcacg acgaagcctt
    43921 gcagcacggt tacgcagcga ttggagatgc gactgggcgc cttcgatttg ggtgccgtcg
    43981 gccgtgagaa ctgttttcct gcgctcttcc tcgtcagccg cggcaagttc ggaggtcagt
    44041 gccagccgcg cgatctcaat gcgcagatca ccgattcggt ccaactcgaa tcgcagcgcc
    44101 tctgaagaag aagcgcgctt ccgcgcctcg aggaacgccg ctccggctgc cgcaagacgg
    44161 cgggtcgcct cgtcaagtcc cgtccgtgcg acggcgatca attcttcgtc atccgcgcgg
    44221 ttcgactcgc ggttgagctt aatcgtccgc tcagcaacga acagtgcaac gtcgtgagcc
    44281 tgattcggat tgggcagcgt cgcggtaatc tccatcacct tcgtgttgcg cacgatggaa
    44341 accctcagca cctcgttctt caacttctcc aaaggcttcg aggtgttcgc gcgcaggcgg
    44401 aagcgctctg ccgcttttgc gaagatctgg tcgcttgatg cgaggtgttc gtaggtgcgg
    44461 agcgattcga ggtagacggg gctgacagcc gtggaggcgc ggacgtcgct accggccggc
    44521 ggttcgatga cgagtaagac ccgggccgtg tacttcttcg gcagcaacag gctgataatg
    44581 agcgtgctgg cgagcgccgt tgcgagcacg accgccgcca gccgccagcg cgagcggatg
    44641 tgcgagaggt attgccggcc gtcaaacgga tcgggcatcg gcacctcccg aaggttgcca
    44701 gaggtgacac ccaagaaacg taatggggcc gagatacgga cctttcatgg ccggccgaga
    44761 cgccggcttc gaaacagcag cggcaatatg cgtcttagtg tgccgcattg cccagcgcgc
    44821 caatcttgcc tatgattgca cgatagactc ggggtctcga ttttgtcaac cattcacgtc
    44881 ggaacacttg taaattcgag atccgggatc gggatgcccg gttttgcgat cgtcgccgcg
    44941 gtgcttgcgc tggctccgct cctgatcctg cccggaagtc tgctttattt cgatgtgacg
    45001 ccaaaagtag ccgtggtagt gctggggaca gcggtggtgc tgcttttggg gagtggaccg
    45061 ccattggaga gaaccggcgc cacaggctgg ttcggcatgc tgcttgttgt gcaagcagcg
    45121 tctttgctgc tttccaccgc gctgtcgacg cagcgttggc tctcggtcac tgggacgaat
    45181 tggcggcgct tcgggctggt gacacagtta tctgtggtgg ttttcgtctg gttagtcgcg
    45241 cggtgcatgg gggccagccc cgccgtacgt gtactccata tcctgcggtt gattgcagcg
    45301 tgcggcgcga tggcagcgtt ttacggcatt cttcagtact tcgggtggga cccatggctg
    45361 ccggcgagtt tctatcatgt cggcgaagga atttggacca tcgtacgtcc gccaggaacc
    45421 ctcgggcacg ctgtgtattt tgcgaactgg ctcctctatg cgttattcgc cggaatggga
    45481 ttgctgctcg ccgagcgggc gcggacctgg agactactgg gcgcggccgc ctgcgccatg
    45541 tgctcgctgg cgatcgtgct tagcggcacg cgtgccgccg tggttggact cgccgggggc
    45601 gcggtgtttc tgcttttccg ggttcggccg cgattgcgtc tgcgtgcgct cggggcgact
    45661 gcgctgctgc ttgcggggtt ggctgttttc tatttctcag cgccggggca gaaactgcgg
    45721 gcgcgcgtgc gctggtcgtt cgaagacccg cgcggcgggg cacgcctgct cctttggaga
    45781 gacacgttgc gtatggcggg ggagcactgg gcagtgggca ccggaccaga gaccttctct
    45841 gcgcagtttc ccaggtacca atccgcagat ctggcgcgcg cttatccgga tttctatcac
    45901 gaatcgccac acaacatact tctcgacgcg ctggccacgc aagggatctt gggtctcgct
    45961 gtactcctgg cgttctgcgc tctggcattc cggaccggat ggcgggcgca catcacttat
    46021 ccggccgtct ccggcgtcct tggggcgatg attgtggccg cgattgttag ccagcagttc
    46081 actgtgttca tacttccgac cgcgctgtac ttctacctga ccgtggcgat gcttatcgca
    46141 ctgtcagcag gagacgggac gggaaaaacg gcaagccccg gcagtgtgta ccgagtcgtg
    46201 tcactcgtgg tccgtgtttc ggcagcggcg ttgttcacct tgttcgcagt gcgtctcatt
    46261 gtggcggacc gggcgctggc gcttacccgg actgaactcg aggcggggcg actcgatgtg
    46321 gcgatcgagc agcatcgccg ggcattggag tggcagcctc caggcatgaa cgctgagttg
    46381 tggtactcgc gcagcctggc ggcgcttgcc gggaattcgc ctaacttgct tgtgcgattg
    46441 caggcgatgc accgggcgat cgaggctgga gagcgctcga cccgctcgtc cgaggaaccg
    46501 cctaacgcct ggtacagcct cgcagcccta tatgccgtgc agaatgacgc cgccggggta
    46561 gagcgatgcc tgcgcaatgc gatcagcgcc tctccgaaat ggtacaagcc tcactgggtg
    46621 cttgcacagg tgttggcacg gaccagccgt ctcaaagaag cgaggaagga ggcggcgaca
    46681 gcggtggacc tgaatggcgg caggaatccg gaagtaggtc ggaccttgga cgcgattgcc
    46741 gccagccccg atcctccgaa ccgctgaatc ggcagaccga ggaagcccct ccacagtaac
    46801 attcgttaat ttacggcagc cgcccttgtg tctctgaagg ccggagcgta aactcgcaag
    46861 aggagggatt tgtgcgcaat cgaggacttg tattgctcac gctcacgtgc gcggttgccg
    46921 cctacagcca gacgccggca gtgttcgatg gcggcgtggt caacgcagcc agtttcgctt
    46981 ccgggcaggt ggtaactcca ggatcgctcg tttccatctt tggtacggaa cttaccgggg
    47041 ctcttgcgct cgcagatagc attccactgg cgacccaact gttgaacgtc tcggtgacgt
    47101 ttaacaacat tcccgcgccg ttggtcgcag tcattccagg aggttcggcg caaatcaacg
    47161 cacagctccc gtggaatgta ctgccagccg gcgagaagac cggaacggcc aacgtggtgg
    47221 tgacgcgcgg cggtgtttcg tcggccccaa aggtggtaca agtcggccca ttctcgcccg
    47281 gcatcttcgc cgtcaatttc ggcatcggtc cggcgatcgc gatcttaccg gacggcgcgc
    47341 tggcggcgcc ggcgggatcg ataccgggac tagcgactcg tccagccaag gttggtgacg
    47401 ttctggaagt cctggctacc ggcctcggcg aagtggatat accggtggcg aatgggcata
    47461 actctctgga tcacctccgg cagacgacca cgaagccgac cgcgctcgtc ggcggtgtgc
    47521 cggtcaaagt cgaattttcc gggatgtctc cgcagttcgt cggcgtgaac caggtcaata
    47581 tccggatcga caacccgaat gttcccaagg gaaatgccgt gccgctacag cttcaggtcg
    47641 gcggcatcac cagcacggat aaagtgacga tcgcggtcga gtaggcgatg cgcggcacgc
    47701 ccggacccgt gctctcggtc gaagaatccg tggaaaccga aacgcggatc ttcgatccat
    47761 tcggccaggg aacggacctc gctcatacag cgaacttcca tccgctggga tttcctctcc
    47821 agctagcgac gaactccgcc gatgttttga cggccgcccg cgagaactgg agtggtttca
    47881 tcccgtgctt tgacgtgcca cccttgcggc tgcacgtcgc agtggatgag gagaaccccg
    47941 gcccctgtcc gacgagcctg atcttccgcg cgcaacggta tttgcttacc gtgatctccg
    48001 accccagtaa cttcgcggtc tgcgatctcg aacggggatt cgcgtcctgc tggttcactc
    48061 gtgccaccgc cagcaatcgg gagtggttgc gttacttcta tctggacacc gtcgcttatc
    48121 tcatcctttg gcaccagcac ctgacacgca ttcatgcgag ctgcgtcgct cgcaatggcc
    48181 gaggtgtcct gctgtgcggc ccttcgggcg ccggtaagag ctgcctcgct tacgcctgca
    48241 cgcgacgcgg ctggacattt atctccgacg aagccacctc gctgatgcga aggtcgagtg
    48301 agcgcgtcgt gctgggaaat ccgcggcgca tgcggtttcg cgaaacggcc gccgacgtcc
    48361 ttccggaact cagtggaaga gtggccgcgc cgaatccggc cggcaagatc acgatcgagg
    48421 ttccgacaag cgatttgccg ggcatcataa cggcgttcca gtgccgcgcc gccgcggtag
    48481 tattcctgga ccgtcacccc ggcggcccgg cgcgactggc gccgctctcg ccggaagaag
    48541 gctggagcag gctcgatcag gacctgcccc tgttcgatca ttcggttcac ctcgaacacc
    48601 gctcatcgtt gaagcgcctg ctcgaaaccg gggtttatga gttgcactac tgtgacctgg
    48661 atcgggcggt cgaactcctg gaggagctcg tccgctgatt catgccgcca tgattctgat
    48721 cctgttagtt ctcgcagcgg cgtttccggt ctggggccag attgcctccg ggtccgtact
    48781 cggtgaaatc cgtgacgaat cgggagcggt cgttccgggc gcacgagtca ccgcccggca
    48841 tgaggccacc ggcttcgcac gcagtgtctt caccggcccc gaaggcgtgt atcagatcga
    48901 gcagttgctg cccggtccct gcacggtgac cgcggagaag ttcggctttc agaccgtgat
    48961 tgcaggtccg atcgtgctgg aagtcaacca gcgggcccgg cttgacctcg tactgaaggt
    49021 ggggatagag agagaatcgg tgacagtgac gacggccgtg tcgccgctac gtgcggacga
    49081 tccttcggcc ggtcaccgtc tcgacgccgc ctcgatttca tcactgccac ttgaccagcg
    49141 aaacgtcatc tcgctggcgt cgctgggccc gggggtaatt ccgcgggccc tggggggctt
    49201 cgttcacgac attatcaccg acgtacaacc aggacgcggc gcagtggcgc tgaatccgtc
    49261 ggtgaacggt gcacgttccc cggcgaacgc attcctcttg gacggtgctg ccgacacgga
    49321 caggctcgcg ttcgtaatct ccgtggtccc gccgatggag tcggtacagg agtttcgcat
    49381 gcagacgtcg ctgccctcgg cggaattcgc acagactgcc ggcggggtcg tagacgtagt
    49441 gactaaggcc ggggcccgcg agtggcacgg caacgcgttc gagtacttcc ggaacgaagc
    49501 gctcgatgcc cgcaatttct tcgacgatcc atcgctgcca cggcccatct tccggcagaa
    49561 ccagttcggg ggctccctcg gtgggccggt tccgctgccg tccaccttct tcttcgcggc
    49621 ctatgaaggc ctgcgaggga aatccgccaa gtcgtccctg aatcttgtac cggacacaac
    49681 cgtccgcggc ggcgattttc gcgggcgaaa cccgattttc gatcctctcg accgggaccc
    49741 ggtgaccgga gcgcggcagc cgttcccggg taacgtggtc ccgctggagc gaatcgaccc
    49801 cgtcgcacgc aggttcctcg atacctacca gccgttgccg aatcggagcg gctccagtgg
    49861 caactacctc gacgccacgc cgaatgagaa caccgacgac aatgtttcag cacgcatcga
    49921 ccatgagcta cacggaggca gccgggtctt cggccgttac acgctcaacg agcagcgcgg
    49981 ccgcttcgcg gggagtttcc ccgtccgccc aaccgaagag cgaatccgcg cgcaacaggc
    50041 cgcactcggc ctcaccttcg gcagatcctc gtggctcaat gaagcccgag tttcgttcac
    50101 gcggctacgg gtttttgacg ttccagagag tgcgttcaag accaatgtcg cgaaggagct
    50161 tggcatctcg gggattcctg acgacccgtt tacatacggc ttgccgtact tcttgattac
    50221 caacttctcc acggtgaccg actcgccgac cctgccgctg acgcagcgtg acaacctctg
    50281 gcacttctcc gacgaggttt ccgtcgcccg cgggaggcac acgctgcggt tcggtttcga
    50341 atgggtgcgg tttaacctca attacctcca gagtcaatta gcgcggggac agttcatctt
    50401 cacgggcgcc ctcaccggcg acccgtcggc gcccgggacg actggcgacc cgctggcgga
    50461 cttcttgctc ggtttcccgc aaatcacaag ccgcaacgtc ggcaccacgc tggcatatct
    50521 tcacaataat tccgcagcag gctacgtgca gagcgactgg cgatggacaa gccgcctgac
    50581 gctgaacttc ggcctgcggt acgagtacac gtcgccgttc acagaggggc gggaccacct
    50641 gcaaaacctg gactattcaa cgctgccggc ggcgccgcga ctggtgcccg tgaaatacgc
    50701 cgtcgatccg gaccggaaca acttcgcgcc gcgggtgggg cttgcattcc ggcctttgcc
    50761 gggccgcgag ctcgtgtttc gtgcgggcta cggcatctat ttcagtcagg aaatggccat
    50821 tgagtcgtac gatctcatcc ggaacggcgt gcgcaacgaa aacaacgtga ccgacggaat
    50881 cacgccggtg ctgacgcttg agaacggctt cccgcagaca gcgtcgaccg gtctgccggg
    50941 ctacttcgga attgaccgcc atgcgcggac gccatacgta cagcagtggt cggctggatt
    51001 gcagaagcaa ctgccggaag ggatcgtcct ggacgtctcc tacgtgggaa cgaagggcac
    51061 gcggctggga cggttccgca gattcaacac gccgcttcgc gtcgagaccg gtgaaaacct
    51121 accgccgcga cccggtgacc tccaacagct ccggccgttt cctgagctcg gcgagatcat
    51181 tcagcggcag cacatttcga actccatcta tcattccctt caggtcaaag tagaaaagag
    51241 gatggccagc cgactaacct tcctcagcag tttcgtctgg tcgaaatcca ttgacgatgc
    51301 cgacagcgtg attccgggat tctttgagag cttcggcgcc caggacgagc ggaacctgcg
    51361 actcgaacgc ggcctgtctt tcttcgatgt aggccggcgc attagcgcgg gcttcgtgta
    51421 cgccttgccc gcctcggcct tcctgcggcc cgtgctgtcc ggctggggga tgagcgggat
    51481 cgtgactctc caggacggaa cgccgctcaa cccggtctac ttcgcctttg atcccgccaa
    51541 ctcgggcacg ccgaaccggc cggacgtggt acccggagtc agcgtgaggc tcccgcgcag
    51601 ccaacggacc gccgaccact tcttcaatac ggatgccttc cgtgcccccg cgccgtacac
    51661 tttcggcaac gccggcagga acattctgcc gggcccgggg aataatgtct tcgacctggc
    51721 gttgcaccgc caatttccgg tacgggagtc gaaggcagtc gagtttcgcg cggagttctt
    51781 caacgcgttc aatcatccga acttcggcat accgggaccg tatccggact ttggaccgtt
    51841 cttcggcaag atctttgcga ctggccagcc gcgccgcgtg caactagcgc tccggattga
    51901 cttctgactt gaaaccgatc ctgtgggagg agaaccggcg cgtcccggcc ggcgtccgag
    51961 cggcgctggc cgcattgcga tttgcgcatc ctgacactcg ggggctgcaa gttctctgcg
    52021 agcgcgaatg gaaggaggca ctccacttct gcgaccgctc gcagacaact ctgttccttt
    52081 gggagcgatg cgaggaggaa tttcccgagt gggtccgcga gcgcgtcgga cagaatcttg
    52141 cggacaacac ggagcgaaac accaggattc gggaggatgt cttcaagatt tgctgcgcgc
    52201 tcgacgggtc tcggactgag tacgtgatgc tgaagggcct gacgcagtgg ccgcgatacg
    52261 tggcggattc gcggctgcga gtccagtacg atcttgacct ttactgcccg cccgattcgc
    52321 tgtaccgggc gcgcgacgta ctgctcgaac tcggatacga gccagccgaa ggcttcgaga
    52381 acttccccgt agaccacttg ccgacgatga tccggaagac cgggttcgag tggcacggga
    52441 actacttcga ccctgacgtg ccggtctgtg tggacgttca tttccggttc tgggatgagg
    52501 agaccgagcg gtttgccgct ccaggactcg atgagttttg ggagcggcgg accaaacatc
    52561 cggtagacgg gcgttgcgtg cccgcgctcg accccgcaga cagcctggca tacacgagcc
    52621 tccacctttt gaggcacctg ctgcgcggaa gcctgcgcct gtgtcacgtg tatgagttgg
    52681 cccgcttcct cgagacgaac gcggacgata gggctttctg ggagaagtgg cgggacttgc
    52741 acccacccgg tctgcggcgg ctggaagcga tcgtgttccg gctggcagcc gaatggttcg
    52801 actgtggtgt gcacgaaacg gctgaagccg aaatcgcaga agttcccaag ggcgttcaat
    52861 tctggttcga gcgttatgcc gcttcgccga tggagggtta cttccggccg aacaaggacg
    52921 aggtctggct gcatctctgt ctactcgatg ccggcaagtg gagcgttctc aagcggcgtc
    52981 tgcttccgtc gcgtctacct gcacgggcag aacccggcaa cttgccggcg tccgagatca
    53041 ctccgcgcct gcggtggcag gcgcgggcac gttacgccgg gcacgtcgcc gcgcgcgcca
    53101 tgcatcacgc ggttgccttt gcgccggcgc tatggaaagg cacacgctgg tggctggaag
    53161 cagcaggacc cggagcggcc tactggcgct acttgctgac ggcgtcgctc taccaccttg
    53221 gcgtttttgt atttgtcctg ctctacaacc tttacctgtt ggatcgcggt tttcgggaag
    53281 actttctggg gttgatcaca agtgcggcca cgattggcag tctcgcggga acgttgccgg
    53341 cggcttgggg aatccagcgg ctcggccttc gtaaaacagt catggcgtgc attgtgggga
    53401 cagcggtggt ctcggctgcg cgtagccttg ttctcgggcc ggcgtcattg gttgcgacag
    53461 cgtttctagg gggtgcgctc tcgtcggtct ggtttgtgtc ggtcgcaccg gcggtgagcg
    53521 gtctatcgag tgagcggaat cgacctgtcg gcttcagcct gttcttcgcc accggcattg
    53581 cggtaggtgt ccttggcggg ttggcgggtg gccggctgcc gggatggatc caagagacgt
    53641 tcgccctggg gacggctgcc tcggccaagc aagcggcgtt gcttacaggc tgcgcgatct
    53701 ccgcgctggc gattctgccg gcccggcgcc tgtcgttgcc gaccggcgtt gcgcgcgaat
    53761 cgcgagtcta cccgtggaac ccggttctcg ggcgattctt tgccgccata gcggcgtgga
    53821 gctttgccac gggctgcctc agcccgtttt tcaccgctct tttctcgcgc cactttaacg
    53881 tgtcggacga gcacatcggc ctcgtcttct ccggctcgca actggcgcag gtggcggcgg
    53941 tactcgctgc accgctggtg ctgcggcggt tcgggctggt cgggggcgtt gttgcgatgc
    54001 aagtggcggc cgccgcgatg ctggccacgc tcgctttggg gcctccggta cttgctgccg
    54061 ccgcggccta tgtcggcttc atgtcgtttc agtctatgtg cgagccagga acgtacagcc
    54121 tgttgatgaa ccaggtgggt gaggagcagc gtagtggtgc gtcggcgctg aacttcctgg
    54181 tgatcttctc cgcgcaggcc gcggcggcgg gtctcgccgg gatcgtgatc acgcggctgg
    54241 gctatccggc tatgcttgcg gcggcggcaa ccgtagcgtt gggagcagcg ctcctgttcc
    54301 ggcgcttgct gctcgcgtcc gactcgcgga ggtaactcaa cggcaggtat tacccgtata
    54361 ggcccggcgt cttccgttat cgtggccgaa aggattcgag gggcgggaga gtgtcgttgt
    54421 ccttgaaacc ggaaacaaat tccgacaaac gctcctggag ccggcgccag tattcatact
    54481 cggcgtccca ccgacccagg gccctgcgga ggcggaacgg gtgatattct tcgagccacg
    54541 ccgtccggta gtcctccctc atttcggaga ttgcatccat caggtcgatg gcgcggctgt
    54601 ggtcctgata gctcgcctca aagccaagga agagatacaa gtcggagctc ttgggattct
    54661 tgccgagccg cgtgaagtac ccttcgatct cgagcgcgta gaggtacttc tggccaaggt
    54721 agtcgagcat gcgggcgccc aggagcagac tcgagagagc gggggcggcg ggcttgattt
    54781 ccagagcgcg catcagaagt tcctgcgctg attccgcctg aaggcgcgcc tctctgaact
    54841 ccgcgcgatg cggcgcggcg cgtttcagcc tgtcgggcac cagagggtct tcccacaacc
    54901 ggtgcatcgt gtcacggccc agcgcatcct cgagatgttg acgggacttg ttgatcgcct
    54961 ccagcgctga ggcaacatcg gacgcgatcg cctcgggata aaggccggcc gcgtagtcgg
    55021 aaaagaaccg gctgtggtcc accggctcgg attgccaggc ggccgcggcg ccataggcca
    55081 ttccggggag ggcctgccga tacagcgtct gggcggcgtc gtgccacccc gtgtggatga
    55141 tgcccatgct cccgtgccgg cgggcggcgg ccaggaaacc atcgacgttc gcgaaggtgc
    55201 ggttcaagtc gggcaccact tcgttccacg tccacagacc ggtggcgatg acgtgcggta
    55261 tgcgtccctc cgagaacggc ttgacgaacg atgtgtaatc cttgcgagcc tcgtagtccc
    55321 aggccacggc gatggtacca ggggggagtt ccgaaacgag ctgtggatag cgaacgaaga
    55381 tctcggaccc ggtggccacg tcggcccaga acatcacgcg tttatcgtgc ttcctaacga
    55441 gcgatgagac gaattgaagg agctctatgt acaacttggc aggctcgacg cctccggccg
    55501 cctggctgcc ggccgcctcc agttcgaacg gctcatcgag gccgatgtga aaccacgggc
    55561 tggggaacag acccgccatc tgggcgaccc agtccgcaag cagctcacgc acacgagggt
    55621 gccgagggtt gaactcgccg ccgtggggaa gcgccgcgag atcggcatag cgctcgatct
    55681 ggaagacgtc gtgaaggtga ccgtagtatt cgagacaggg tacgacatcg acatgccgct
    55741 ccctggcgta gtcgatgata tggcgcacct gctcccgcgt gtaccgcgcc ttgggactga
    55801 acagggggta accggtcaac tcgatgctga cttccgaata gaagtaatac tggttcacct
    55861 tccagcgggc gaggaaatcg atctgccgtt tgatctcctc ctctttcggc aggggtccgt
    55921 gactcatgtc catcatgaag ccccgatacg cgagggaagg ccagtcccgt atctcgactt
    55981 cgggaagcac tgcgtgcgcc ccttcgccct ctaccaactg gcgcagcgtc tggacgccat
    56041 agaacaagcc ggccgaggat cgcgcgcgga tttccgctcc gttcgagttg atcttgatcg
    56101 agtaagcctc ccgcgaatcg ggtccggggc gctcgtcggc catgggcaac gccgcaacat
    56161 ccccggttcg ggacagcaag atggtttttc ccgatcgctc gacgtcgagc accggcactt
    56221 gcgtctgagc aatcgaggaa agcgccgcag agagctcctg ggcgacaaac cggtcctccg
    56281 cgccgggcgc ggatgcgaag cggatggaca agccccgcac gggtaactcg gctccgccgt
    56341 agcggacctc ctgcggacgg ggcagcagcg gcttatggct accgggggcg gcagcaagca
    56401 gccacaccgg catcaggcaa gcgagcagca aagatttcgg catgacgttt tacctcagag
    56461 tcggatgaac gtcgcaatgg cggccactat gacgccgctt ccatcgcggg agcgcccttc
    56521 tttgcagagc gcgatagttc gcgaagcacg acttcggccg cctgtagagt ccggtcaacc
    56581 atctcggcgg tgtggctgag actgatgtga ttccgcttca gattcatcgg caacttgaaa
    56641 atgccgcgtt cgagaagctt gcgccggtag gagacgaaca tctcggcgtc gttcctggtg
    56701 aggtctgtgt agctctcaat cgggccgtcc ataaagtagg tcaggaatac ggaaccgaag
    56761 ccggtgacgg tagctttcag cccgagccta gtcacaatgc cgctgagccc ttcccgcatg
    56821 cgttcgccta ggccgaacat gtgacgataa gtggattcct gctcaagcac ctcgatggta
    56881 gcgagtgcgg ctgcacagca gacggggtga ccgttgaacg taccggcgaa gaacacatcg
    56941 ccatcggagt gggtgttgaa tcggtccatc agttcccgct tgccgcagat gacggaaatc
    57001 ggatacccgt tggcgacggc cttcccaagg gtggtgaggt ctggtgtcac gccgcagatc
    57061 ttctgatagc cgccgagacc gtgccggaag ccggtgatga cttcatcgaa aatcagaacg
    57121 atgtttttca gacgggtgag ctcgcgtaga ccctcgagaa agccgggctt cggaagcaca
    57181 cacccgatgt tgtgcggaat gggctcgagg atgatggcgg ctacctgtcc cttgttggcc
    57241 gcgaccgtcc gctcgacctc gtcgaggtca ttgaacgtgc agaccagggt gtgttcgacg
    57301 acctcgggca ggcttcctgc ggacaggagc tgtcgagatc ccaactgttc ggccggagtg
    57361 atcacattca tcgcgacggc gtcatgccac ccgtggtagc agccttgaaa cttgatgatc
    57421 ttctttcgtc cggtgacggc gcgggcgagc cgaatcgcct ggtaggtcgc ctctgaaccg
    57481 ctgttggtaa agagcactct ttccgcggac ggcacgtgct ggctgatctt ccgagccagc
    57541 gcaatctccg attccgttgt cccgacaccg agaagatcga tattttgcat cgtctcttgg
    57601 acccgccggt tcaccatttc atgattgtgg ccgagtacga tcggaccgaa agcggcctgg
    57661 tagtcgataa attggttgcc gtcgacgtcg gtcatcgttg cgccttccgc tcgcgcaaag
    57721 acaagatgcg gctcgaaccg tctcaaagag gtgttgacgc ctcccggcat cacatcactt
    57781 gccagggcgt atttcaggcg gttttggtca ttcgttggca tcggcttttc cttagcttcc
    57841 ccgctcggga cctcaagtta ttgcgaattc taatccagcg acgcaataac gtccaatacg
    57901 aaacatgacg ggaagtgcgc ggccatccag aagcttggac gaaaaccgcc tcggcgactg
    57961 cggggcagca cttcgtgctg cgggatgcta tgcggttgac aggagcagtg catcaagcta
    58021 ggattccagc gagatctcgt gttcaggctt cagggtcgtt ctgggttacg ttgctggacg
    58081 ccaacaggcc tcttgtgtgt ctttctccag gcggcgacgc tggcatgtac gtccggatcc
    58141 accgcggcgc aacaaggggt acgtgcggcg ccattgcccg accttgcccc gctcgaacaa
    58201 ctgaagctgg acgaatttga acccgcggtc ggcgaacaga ttcgcggggc gtatgaaagg
    58261 gccagccagc atccccgaga cgcggcggca gccggcaaac tgggcatgat tcttcaggtc
    58321 tacggaaagt acgaactggc ggagcgctgc tttctccgtg caaaagggct ggatgcgcgt
    58381 tcgtttcgtt ggacatacta cctcggcaat gtggagggtt ttcttggaaa acatcccgac
    58441 gccgtccagc acgtccgcga ggccttgacc attgacggac aatacgctcc tgcccgggtg
    58501 cgtctggcgc agttgttatt ggactccggc gacttggagc aagccggcca gctttatcgc
    58561 gaagcggccg atcagaatcc cgaactagct tccgcccatt tcggcctcgg tcaggtgctg
    58621 gcggcccggg aggagttgga ggcggccatt gagtcgtaca aacgagcctg tcagctcgcc
    58681 aagaattatg cggccgccca ctatgcgctc gggatggcat acagaaagac aggtgatctg
    58741 gccaacgccc gcacgcatct ggaactatat cagcgtctca agcaggcgaa acagccggtg
    58801 ctcgatccgc tcatggagga ggtgaatgcc ctttattccg gagggctcac gcacttcgcc
    58861 aagggctctg ctttggcgca gcagggaaaa ttacaggagg cggccgctga gttcgaagcg
    58921 gcgctgaacg tgaatccggg gatggtgatg gcacacatca acctgatcgc tatgtgcggg
    58981 caattgggcc agccggagaa agccgaacaa cacttccgcg cggcagtcga gctggaccca
    59041 ggttgggtgg agtcctatta caactggggg atgttcctgc tccagaaggg aaaaaaggcg
    59101 gaggctgcgg agatgtttcg caaagcggtc gcggtcaatc caagctatcc ggatgcacag
    59161 gtccaactag gatcgttgct cgacgaatcg gggcgcgcgg cggaagctgc cgctcattac
    59221 gagcgagcgc tcgccgccga cgctggccac cggcaggctc attaccttct cgctcacaac
    59281 ctggttcgca ggggacaggt ggacgacgcg atccggcatt tcctcgagac gatcaaggtg
    59341 gaggacaacc gcacgccctt ctgcatgtat ggcctagcag tagcatatga acgctccgga
    59401 gaccgccaac aggctcttta ctacatggag caggcgcacc ggcgcgcggt ctccttcgga
    59461 atgcgagacc tagcttcacg gctccaacag gacttgaagc gtttgcaggc ggagggtgtc
    59521 cgccgttgac tgcgcctctt cggaagcgtc ccggcgcctt gtcgggccct cgaatacgcc
    59581 accgctgtgg ggcgtttcta ctcgtggctt tcgcggcatg ttgcgtggca actgccgccg
    59641 atgtcctgtt cgaggacgcc gctgagaaga caggccttcg attccaacac ttcatcgggg
    59701 cgaccggatc ctatttcgtg cccgagattt tcggctccgg cgtcgcactg ctggattacg
    59761 acggggacgg tgatcttgac gtctatttcc tgcaaggggc ggtgctcgat aaaacgaagt
    59821 cgttgaagga ttcgttattc cccctaccga aggaacattt ccccggcaac cggctgttcc
    59881 ggaataacct gataccaacc gggacgctgt ccttcacgga tgtgacggag caagcgcacg
    59941 tcgcaggaaa tgggggatac gggatgggcg cggcggtggg ggattacgac aacgacggcc
    60001 acccggactt gtttgtcaca aactacggca gggacatcct gtaccataac aacggcgacg
    60061 ggacattttc cgatgtaacc aaagaggcgt ttccggcgga ggagtccttc ggggcgagtg
    60121 cggcgtttgt cgactatgac cgggacgggt tcctggacct ggtcgttgtg cgttacaact
    60181 ccttcacggt tcaaggaaac aagcggtgct ccggttatgc gggtgcgcgg gagtactgcg
    60241 gaccgggtga ctatcgcccg atccctgaca agctctatca caacgacggc aaagggcgct
    60301 ttaccgacgt tagtcagaag gcggggcttg gggccgcgtt cggcaacggg cttggagtgg
    60361 tatgcgctga ctttgacggc gacggctgga tcgacattct ggtcgccaac gacaagactc
    60421 cgaatcaact ctggatcaat cacggcaacg gcactttcga agacgacgca ctgatgtcgg
    60481 gaacggcttt tgacgacgaa ggtaaagcgc acgcggggat gggagtaacg gcgggcgact
    60541 tcgacggaga tggtgatgaa gacatcttca tcgccaacat tttgggcgag acgcatatgc
    60601 tgtacctgaa cctgggtaac ggaatcttcc gggatgtaac caagctgtat ggcctcggtt
    60661 acacaacggt tccctacacc ggattcggca ccctctggtt tgattacgac aacgacggac
    60721 gactcgacct gttcattgcc gacggggaag taagggcgat cgaatcgcag cggggcaagc
    60781 cctttccgta cgatcagaag aaccagctct ttcacaacga aggcaagacc ttccgcgagg
    60841 tcacagggga ggctggtccc gtgttcgacc tttcggaggt gggccgaggg gcagcattcg
    60901 gtgacatcga taacgacggt gacatcgaca ttgtcgtcag caacgcgaac ggaccggcgc
    60961 ggttgctgct gaaccagatc ggcaaccgca accactggct ggaggtgcgc cttcagggag
    61021 tcaccgtgaa ccgcgacgcc ttaggcacac gggtggcgtt gcttcgacag ggccgtccgg
    61081 cactttggcg cagggtagcg acggacggca gttacttgag cgccaatgat ccccgcgtgc
    61141 actttggact gggaccggac gcggcattgc gcgtggcccc tccggagaac atcgtcgtca
    61201 tctggccgga cggctcgaag gagacctggc cgattcccgg cgtcgatcgg gtgcttcagt
    61261 ttcggcaagg cacgggtaca caagtgaagg aaactccgac gaccaagccg tagagtcgga
    61321 ttttctatcg ttcgggcggg gctcgcccga tgacttggga gccccgtgcc ctgccgcgcg
    61381 aagtcagtct agaactcgat acgccctcgc aattcgacca tgcgctgccc ggctctttcg
    61441 cccgagtaca gaccgtaggt ttccccaatc acaccaaccc cgcccggtac ggagatgttg
    61501 ttggccggga acaggaagtt cgggtggttg aagatgttgg agatcaaccc catgaagtc
//
LOCUS       DATIFQ010000373         9665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2041424, whole genome shotgun sequence.
ACCESSION   DATIFQ010000373 DATIFQ010000000
VERSION     DATIFQ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041424"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /gene="sufB"
                     /locus_tag="VLE22_18255"
     CDS             complement(<1..848)
                     /gene="sufB"
                     /locus_tag="VLE22_18255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HSB16399.1"
                     /translation="MSTTAQIEALANQEYKYGFVTEIEADSVPPGLNEDIIRLISAKK
                     NEPEFMLEWRLKAYRHWLTMTEPTWANVRYPQIDYQSIVYYSSPKVKKDGPKSLDEVD
                     PKLLETYEKLGIPLRERELLAGVAVDAVFDSVSVATTFKEKLSELGIIFGSFSEAVQE
                     HPDLVQKYLGSVVPHSDNFFAALNAAVFSDGSFCYVPKGVKCPMELSTYFRINAQNTG
                     QFERTLIIADEGSSVSYLEGCTAPIRDTNQLHAAVVELVALDNATIKYSTVQNWYPGD
                     KDGKGG"
     gene            complement(991..1329)
                     /locus_tag="VLE22_18260"
     CDS             complement(991..1329)
                     /locus_tag="VLE22_18260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HSB16400.1"
                     /translation="MRIITILTALLVTFSLAGLAQEKKAPEKLVFQTKMGNVTYDHAK
                     HLERAKGDCKVCHDKLWAQDAKAPLNFKAGMHKVAEKGKTSCATCHVAGGSAFVTTGN
                     CAKCHVKGAK"
     gene            complement(1409..1858)
                     /locus_tag="VLE22_18265"
     CDS             complement(1409..1858)
                     /locus_tag="VLE22_18265"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSB16401.1"
                     /translation="MSLIDTVRSLLNAKGKAVWALPPDATVYEAIELMAEKSVGAVLV
                     ISEGKLAGILSERDYARKVILQGRSSKDTLLREIMSRPVLTVDPGATVDECMRIVTEH
                     RIRHLPVMEGERVVGIVSIGDLVRSIISAQAATIDQLHSYIAGKYPG"
     gene            1932..2945
                     /locus_tag="VLE22_18270"
     CDS             1932..2945
                     /locus_tag="VLE22_18270"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSB16402.1"
                     /translation="MKIPAFEAGLMQRRHFLATLSGGSAMMAYSNLAAAAPDPAARRE
                     ELYGLLGELPGRHRKITVRTISTEERPAYVLEKLELDLNGIEPAPAWFVRPKALSGRV
                     PAILYNHAHGGDYKLGKDEFLKGRDVLQSPPYAELITSLGYCGLCMDTWVFGERATRT
                     ELDTFKHMLWNGKVLWGMMVYDSIRGVDYLSSRPEVDAGRIGTLGLSMGSTMAWWLAA
                     LDERVKVCVDLCCLTDFQALIEAGNLKGHGIYYYVPSLLKHFTTAQINALIAPRPHLS
                     LAGNLDALTPTAGLDRIDRELKAVYAALGKPEHWKLLRYNVGHEETPEMRREIIAFLK
                     QFL"
     gene            2942..4396
                     /locus_tag="VLE22_18275"
     CDS             2942..4396
                     /locus_tag="VLE22_18275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB16403.1"
                     /translation="MSKSRDWSTAGVALAALVIAAYFISFTAGSLRTEFTQDDLMNCY
                     RGWFHPLKALVAENFMFWKFSPTFRPLPALIYKASFGLFGFDLFAMRLLLWGVMAANV
                     VLAYLLVRRLTDSRETGLLAALLICYHSNFASLYYNTGTIYDIFCYFFYVFVLVLYAF
                     WRSRHRLPGPVALVALALLYIFALDSKELAVSLPVTILAYELLFHPPSLRGLGGWLVR
                     EARFAWLTGFITLGFIFGRINGPYSISEQGGYHVAPSAGEYLKQAANYFGELFYRTGW
                     FNSMKALVLLLGLFVVALALRSRVLVFGWIMFTTGFLPMAFIPPRSLHAIHLPLTGLA
                     LYFAALLVDLRDRLLRAARRTSLRPAGQALLFIALTLFLIRVHPGNESNYNAWLKEYR
                     QIRIIQEQYKQLYPSMPPDSRVLILKDPFGQYNWASLFVIYLVYGDLSIHVDRLETME
                     PKPDAAALETYNYILTFEDDRLIGIDPKYVSPAQ"
     gene            complement(4413..4868)
                     /locus_tag="VLE22_18280"
     CDS             complement(4413..4868)
                     /locus_tag="VLE22_18280"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB16404.1"
                     /translation="MNEIYRIRDQMRRAFEGDAWHGPSLREVLEGVDAESAARRPLAG
                     VHSIWEIVLHVSAWKTALCRRIEGSAIELAGEEDWPPVHAGGEAAWKQTLAELAERHR
                     ALYGLTARMSDSRLLDPVPGKDHDVYFMLHGAVQHDIYHAGQIAVLRKA"
     gene            4941..6608
                     /gene="ilvD"
                     /locus_tag="VLE22_18285"
     CDS             4941..6608
                     /gene="ilvD"
                     /locus_tag="VLE22_18285"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSB16405.1"
                     /translation="MKLQSHTITQGRDRAPARSMLKAIGFTDEDLRKPIIGIANTWIE
                     TMPCNYNLRELAVKVKEGVRAAGGTPMEFNTIAISDGVTMGTEGMKASLTSREIIADS
                     IELVARGHMFDGIVALVACDKTIPGAAMALLRLNVPGIVLYGGSILPGHYKGRDLTVQ
                     DVFEAVGANAAGRITDDELLAIENAACPGAGACGGQFTANTMATVMELIGLCPMGMAS
                     VPQVDPRKSDVAFRCGEMILEAVRRDLKPRDIVTRKAIENAIAGVAASGGSTNAVLHL
                     LAMAREAGVPLEIDDFQAISNRTPLLCDLKPAGRFTAVHVDTAGGIPVIAKRLLEGGY
                     ADGAQLTVTGRTFAEEAKLAKETPGQDVIRPLSNPIKSSGGLVILRGSLSPEGCVIKV
                     TGIDKKSHRGPARVFDREEDAMTAVTRGEIKAGDVLVIRYEGPRGGPGMREMLGVTSA
                     IVGAGLGESVALLTDGRFSGATRGLMAGHVAPEAFNGGPIAAVVEGDSILIDIEHRRI
                     ELEVPEATVKQRLAAWKAPKPRYKTGVFAKYAALVQSASEGAITSKL"
     gene            complement(6652..6981)
                     /gene="trxA"
                     /locus_tag="VLE22_18290"
     CDS             complement(6652..6981)
                     /gene="trxA"
                     /locus_tag="VLE22_18290"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSB16406.1"
                     /translation="MAGPNTLTFTDSSFDKDVLTSDVPVLVDFWAEWCGPCRMMEPTI
                     EGIAADYSGKAKVGKVNVDENGGTAMRYGIRGIPTLLLFKGGKVVEQRVGAVGKSEVQ
                     KMLDAHV"
     gene            complement(7026..8444)
                     /gene="glgA"
                     /locus_tag="VLE22_18295"
     CDS             complement(7026..8444)
                     /gene="glgA"
                     /locus_tag="VLE22_18295"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HSB16407.1"
                     /translation="MAKILMVASEATPFIKTGGLADVVGALPAALKARGEDVAVVLPL
                     YRDARVDGARRVAEKLDVWLGLAHYSVNVQMAVERDVPYFFVECPSLYDRKGVYVDAA
                     GDFPDNHIRFAVLAKAALASARHVFRPQILHCHDWQAALAPVYLRTALAGDPTFLGVK
                     TLLTIHNLGYQGIFAPSALPEIGLDPALLTIDRLEFLGDLSVLKGGIVYSDAISTVSK
                     GYAREIQTPEYGFRMDGLLRARSSVLTGILNGVDYSAWNPETDVHLAAHYSLDDLRGK
                     KACKLDLLRQFGLPDEAAGRPLIGIVSRFARQKGFDLIEQAASGLLDEDASLVALGTG
                     EPRYEEMFRNLAAAYPGKVAVRIAYDNPLAHKIEAGADIFVMPSRYEPCGLNQIYSLR
                     YGTVPVVRATGGLDDTIDEDTGFKFSEYSSQALLASLRDALAAWRDRRRWETLMRNGM
                     RKNFSWNASAAEYSALYRRLLA"
     gene            8521..8724
                     /locus_tag="VLE22_18300"
     CDS             8521..8724
                     /locus_tag="VLE22_18300"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSB16408.1"
                     /translation="MKFRVVLDYDPEARSYSAVCPELPGCASAGETEEEAKRGIEEAI
                     RLYFSSAGIELSPTAKLVEVTVG"
     gene            8829..9323
                     /locus_tag="VLE22_18305"
     CDS             8829..9323
                     /locus_tag="VLE22_18305"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HSB16409.1"
                     /translation="MLAGVAAAQDVQYNFDQQVDFSRFKTYKWAEIPGGQKLDDLLSR
                     HIRDAFDAGLAKKGLTKTDADSADLYVGWQVAVNQEKELTAYGTGWGYGPGWRGSGMG
                     TAKTSTILIGTLVLDMYDTQNKQLVWRSSATKTIDTNANPEKRSKNLAKAVDKMLKNY
                     PPKK"
     gene            complement(9359..>9665)
                     /locus_tag="VLE22_18310"
     CDS             complement(9359..>9665)
                     /locus_tag="VLE22_18310"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB16410.1"
                     /translation="IEERHAEGHPFVESLLDAGIMRLRPVLITVGATVIALYPLASHG
                     GPLWEPLCYAQIGGLTIATFVTLLLVPVLYAIFVLDLKLVKWAGPPAEPSAGTASDR"
BASE COUNT         1790 a         2958 c         3018 g         1899 t
ORIGIN      
        1 attccgccct tgccgtcctt gtcgcccgga taccagttct gcaccgtcga gtacttgatc
       61 gtggcgttgt cgagcgccac cagttccaca accgcggcgt gcaactggtt ggtgtcgcgg
      121 atcggcgccg tgcaaccttc caggtagctc accgagcttc cttcgtcggc gatgatcagc
      181 gtgcgctcga attgtcccgt gttctgggcg ttgatgcgga agtaggtcga cagctccatc
      241 gggcacttga cgcccttcgg aacgtagcag aacgatccgt cgctgaagac cgccgcgttt
      301 aatgcggcaa agaagttgtc cgaatgagga accaccgagc cgaggtattt ctgcacgagg
      361 tccgggtgtt cctggaccgc ctccgagaac gaaccgaaga tgatgcccag ttccgacagc
      421 ttctccttga acgtggtcgc caccgagaca ctgtcaaata cagcgtccac cgccaccccg
      481 gcgagcaact ccctctctct caatgggatg ccgagcttct cgtacgtttc gagcagtttt
      541 gggtcgacct cgtcgaggct cttcggaccg tccttcttaa cctttgggct cgagtagtag
      601 acgatgctct ggtagtcgat ctgcgggtat cttacgttgg cccaggtagg ctccgtcatc
      661 gtgagccagt ggcggtaggc cttcaggcgc cattccagca tgaactccgg ctcgttcttc
      721 ttcgccgaaa tcagccggat gatgtcttcg ttcagcccgg gcgggaccga gtcggcttcg
      781 atctcggtga cgaaaccgta cttatattct tgattcgcaa gggcttcgat ctgtgcggtg
      841 gtgctcatcg gatctcccag tgaggcagtc gggacaccct atcacggtat ccctttactc
      901 actgtagcga atcttgacaa actcgtcaag attcctaccg ttaatggatg attctggggt
      961 ccgatcggtc gcaaaggcgc tcgaactagc ctatttcgcg cctttaacgt ggcacttggc
     1021 gcagttgccc gtggtcacga aggcactgcc gcccgccacg tggcacgtcg cgcaggaggt
     1081 cttccccttt tcggcgacct tatgcatgcc agccttgaag ttcagtggag ccttcgcgtc
     1141 ctgggcccag agcttgtcat ggcagacctt gcagtcgccc ttcgcccgct ccaagtgctt
     1201 ggcgtgatcg tacgtgacgt tgcccatctt cgtctggaag acgagctttt cgggagcttt
     1261 cttctcctgt gccaggccgg ccaggctgaa ggtaacgagc agtgccgtca gaattgtgat
     1321 aattcgcatc tgaatctctc tgtaatgtgg accgcgccgg acacgtacgg gtttcaacga
     1381 atttgccccg ccatgccggt ccgtgtcatc acccaggata ttttccggcg atgtaactat
     1441 gcaactggtc gatggtggcc gcctgagccg agatgatcga acggacaagg tctccgatcg
     1501 agacgatgcc caccacccgt tcgccctcca tcaccggcag atgccggatg cgatgctcgg
     1561 tcacgatccg catgcactca tcgacggtgg ccccgggatc aaccgtgagc accgggcggc
     1621 tcatgatctc ccggagaagc gtgtccttgg aggaccgtcc ctgcaggatc accttgcggg
     1681 cgtagtctct ctcggacaga atgcctgcca gtttgccctc tgagatcact agcaccgctc
     1741 cgacgctctt ctcggccatc agctcaatcg cctcgtagac ggtcgcgtcc ggcggcaacg
     1801 cccagacggc tttccccttc gcattgagaa gggatcgcac tgtgtcaatt agcgacatga
     1861 ttcacctgcc cagagtgtac tggtgagatt ctatcaccga tcgctgcacg ccggagccgc
     1921 atctggcgat aatgaaaatt ccggccttcg aagccgggct tatgcagcgc aggcattttc
     1981 tggcgaccct ttcaggggga tccgcaatga tggcttactc aaacctggcg gccgcggcgc
     2041 ccgaccccgc cgcgcgccgg gaagaactgt atggcttgct cggggaactt cccggccgtc
     2101 accgcaagat caccgtccgg accatctcta cggaagaacg gccggcgtac gtcctcgaga
     2161 aactcgaact cgacctcaac ggcatcgagc ccgcgccggc gtggttcgtc cggccgaaag
     2221 ccttaagcgg ccgcgttccg gcgatcctgt ataaccacgc gcacggcggc gactacaagc
     2281 tcgggaagga tgaattcctc aagggccgcg acgtgctcca gtcaccgccc tatgccgagc
     2341 taatcacctc gctcggatat tgcggcctct gcatggacac atgggtcttc ggcgagcgcg
     2401 cgacccgcac ggagttggac accttcaaac acatgctctg gaacgggaag gtgctgtggg
     2461 gaatgatggt gtacgactcc atccgcggcg tcgactacct gtcttcccgg cccgaggttg
     2521 acgccggccg gatcggcacg ctcggcttgt cgatgggcag cacgatggcc tggtggctcg
     2581 cggcgctcga cgagcgtgta aaggtctgcg tggacctttg ctgcctcacc gatttccagg
     2641 cgctgattga agccggcaac ctgaagggcc acggcattta ctactacgtt ccgtcgttgc
     2701 tcaagcactt caccaccgcg cagatcaatg cgctcatcgc tccccggccg cacctgagtc
     2761 tcgccgggaa tctggatgcc ctcacgccca ccgccgggct ggaccggatc gaccgggagc
     2821 tgaaagccgt ctacgcggcc ctcggcaagc cggaacattg gaagctgttg cgttacaacg
     2881 tcggtcacga agagacgccg gagatgcgtc gcgaaatcat tgcctttctg aaacaatttc
     2941 tgtgagcaaa tcccgagact ggtcaacagc cggcgttgct ctcgccgcgc tcgttattgc
     3001 tgcttacttt atttcgttca ccgcgggaag cctgcggacc gagttcacgc aagacgacct
     3061 gatgaactgc taccgcgggt ggttccaccc gctcaaggcg ttggtggcgg agaacttcat
     3121 gttctggaag ttcagcccga cgttccggcc gctcccggca ctgatctaca aggcttcgtt
     3181 cgggttgttt gggttcgacc tgtttgcgat gcggctgctc ttgtgggggg tgatggccgc
     3241 caacgtggtt ctggcctacc ttcttgtgcg gcgcttgaca gattcccggg agacgggcct
     3301 cctcgccgca ttgctgattt gctaccactc gaacttcgcg tcgctttact acaataccgg
     3361 cacgatttac gacattttct gctacttttt ctacgttttt gtgctcgttt tgtacgcttt
     3421 ttggcgctct cggcaccgtc ttccgggtcc cgtggccctt gtagcactcg cccttctcta
     3481 tatcttcgcc ctggactcga aggagcttgc ggtctcgctt ccggtcacga ttctcgccta
     3541 cgagctgctc tttcatccac cgagtcttcg cggcttgggc ggctggctgg tgcgggaggc
     3601 ccggttcgca tggctgacgg gtttcatcac gctgggcttc atcttcgggc ggatcaacgg
     3661 accgtactcc atcagcgagc agggcggata tcacgtggcg ccgtccgccg gcgagtacct
     3721 gaagcaggcg gccaactatt tcggcgagct gttttacaga acaggttggt tcaacagtat
     3781 gaaagcgctg gtgcttctgc tcgggctgtt cgtggtggcg ctggcattac ggtcgcgcgt
     3841 gcttgtcttc ggatggatca tgttcacaac tggcttcctg ccgatggcgt tcatcccgcc
     3901 ccgcagcctc cacgcgattc acttgccgct gacgggcctg gcgctgtact tcgcggcgct
     3961 gctggtggat ttgcgtgacc ggttgctgcg cgccgctcgc aggacgtcgc ttcgccccgc
     4021 tgggcaggcc cttctgttca tcgctctgac tttgttcctg atccgtgtgc atccgggcaa
     4081 cgagtccaac tacaacgcct ggctcaagga gtaccggcaa atccgaatca tccaggaaca
     4141 gtacaagcag ttgtatcctt ccatgccgcc ggacagccgg gtcctgattc tgaaagaccc
     4201 attcgggcag tacaattggg ccagcctctt cgtcatctat ctcgtctacg gcgacctaag
     4261 catccatgtg gaccggcttg agacgatgga gccgaagccc gacgcggcgg cgctggagac
     4321 ctacaactac atcctgacgt tcgaagacga ccggctgatc ggcatcgatc caaaatacgt
     4381 ttcgccggcg cagtgaagcc gcgaggctgc cgtcaggcct ttcggagcac ggcgatctgg
     4441 ccggcgtgat agatgtcgtg ctgaacggcg ccgtggagca tgaagtagac gtcgtggtcc
     4501 ttgccgggga cggggtcaag caggcgggaa tcgctcatcc tcgcggtcag gccgtacagg
     4561 gcccgatggc gttcggcgag ctcagccagc gtctgtttcc aggcggcctc tccgccagcg
     4621 tgcaccggcg gccagtcctc ctcgccggcc agctcgatcg ctgacccctc gatccggcgg
     4681 cacagcgcag tcttccaggc ggagacgtgc aggacgatct cccaaatgct atgaacgccg
     4741 gccagcggcc gccgcgccgc gctctcggcg tccacgcctt cgagaacctc acgcagcgac
     4801 ggaccgtgcc aggcatcgcc ttcgaaggcg cgccgcatct gatcgcgaat tctgtagatc
     4861 tcgttcattc agacagcctc ctccgcaatc ctaccgcctg acgggcgcga ccgaggcggt
     4921 tagaatggga gacacctcct atgaaacttc aaagccatac cattactcag gggcgggacc
     4981 gggcaccagc ccgctcgatg ctgaaggcca tcgggttcac cgatgaggac ctgcggaaac
     5041 ccatcatcgg catcgccaac acctggatag agacgatgcc atgcaattac aacctgcggg
     5101 aacttgcggt caaggtgaag gaaggagtcc gcgccgcagg cggcacgccg atggagttca
     5161 acacgatcgc gatcagcgac ggcgtgacga tgggcaccga ggggatgaag gcgtcgctga
     5221 ccagccgcga gatcatcgcc gattcgatcg aactggtcgc gcgtggccac atgttcgacg
     5281 gcatcgtcgc gctggtggcc tgtgacaaga cgattcccgg agcggccatg gcgctgttgc
     5341 ggctgaacgt gccgggtatc gtgctgtacg gcgggtcgat tctgcccggc cactacaagg
     5401 gccgcgacct gacggttcag gacgtctttg aagcggtcgg agcgaacgcc gccgggcgca
     5461 tcaccgacga cgaactgctt gccatcgaga acgccgcctg tccgggggcc ggggcgtgcg
     5521 gaggccagtt caccgccaac acgatggcga cggtcatgga gctgatcggc ctctgcccga
     5581 tggggatggc ttcggtaccg caagtggacc cgcggaagtc cgacgtcgcg ttccggtgtg
     5641 gcgagatgat tctagaagcg gtccggcggg acctcaagcc gcgagacatc gtcacccgaa
     5701 aggcgatcga gaacgccatc gcgggggtcg cggcgagcgg aggctcgacg aatgccgtac
     5761 tgcatctgct ggcgatggcc cgcgaggccg gtgttccgct cgagatcgac gatttccagg
     5821 cgatcagcaa ccgcacgcca ctgttgtgcg acctgaagcc cgccggccgg ttcaccgcgg
     5881 tgcacgtcga cacggcgggc gggattccgg tgatcgcgaa gcggcttcta gaaggcggct
     5941 acgccgacgg ggcgcagttg acggtcacgg ggcggacttt cgccgaggag gccaagctgg
     6001 cgaaggagac cccgggccag gacgtgatcc ggccgctctc gaatccgatc aaatccagcg
     6061 gcggactggt gatcctgagg ggcagcctct caccggaagg gtgtgtcatc aaggtgaccg
     6121 ggattgacaa gaagtcgcac cggggcccgg cgcgtgtttt cgaccgcgaa gaggacgcca
     6181 tgacggcggt gacacgcggc gagatcaagg ccggcgacgt gctggtaatc cgctacgaag
     6241 gcccgcgcgg cgggccggga atgcgagaaa tgctgggagt caccagcgcg attgtcggcg
     6301 cgggtctcgg cgagagcgtg gcgctgctca ccgacggacg tttcagcggg gcgacacgcg
     6361 gtctcatggc gggacacgtc gcgccggaag cgttcaacgg gggtccgatc gccgccgttg
     6421 tcgagggaga ttcgatcctg atcgacattg agcaccggcg gatcgaactc gaagtgccgg
     6481 aggcgaccgt caaacagcgg cttgcggcct ggaaagcgcc gaagccgcgc tacaagacgg
     6541 gggtgttcgc gaagtacgcg gcgctggtcc agtccgcttc tgagggggcg attacttcga
     6601 aattgtgaag ggtgatcggg ccattcaccc gatcaccgca tcacggaagg ttcagacgtg
     6661 cgcgtcgagc attttctgga cttcagattt gccgacggcg ccgacgcgct gctcgacgac
     6721 cttgccgccc ttgaagagca acagggtggg gataccgcga atgccgtaac gcatagcggt
     6781 gccgccgttt tcgtcgacgt tgactttgcc gactttcgcc ttgcccgagt agtccgcggc
     6841 gatcccctcg atggttggct ccatcatgcg gcacgggccg caccattccg cccaaaagtc
     6901 caccaggaca gggacgtcgg acgtcagtac atccttgtca aaacttgaat ctgtaaaggt
     6961 tagagtattc ggacctgcca ttccatctct ccacttcgat cagatgcatt cttggcgaaa
     7021 aagattcagg ccagcagccg gcggtagagc gccgagtact ccgcagccga ggcattccag
     7081 gagaaatttt tccgcatgcc gtttcgcatc aatgtctccc agcgccgccg gtcacgccag
     7141 gcggccagcg catcgcgaag cgaggccaat agagcctggc tgctgtattc gctgaacttg
     7201 aatccggtat cctcatcgat cgtatcatcc agcccgccgg tggcgcggac taccggcacc
     7261 gtgccgtaac gcagactgta gatctgattc agcccgcagg gttcatagcg gctgggcatg
     7321 acgaagatat ccgcgccggc ctcgatcttg tgcgccaggg ggttgtcgta ggcgatgcgc
     7381 acggcgacct tgccggggta agcggcggca aggttccgga acatctcctc gtagcgcggt
     7441 tcgccggttc cgagggccac caggctcgcg tcctcgtcca acaacccgga agcagcctgc
     7501 tcgatgaggt cgaaaccttt ctgcctggcg aaccgcgaca caatgccgat gagcggccgg
     7561 cccgcggcct cgtccggcag gccgaactgg cgcagaaggt cgagtttgca ggccttcttg
     7621 ccgcgaaggt cgtccaacga gtagtgcgcg gcgagatgca cgtcggtttc ggggttccag
     7681 gcggagtagt cgaccccgtt cagaatgccg gtaagcacgc tgcttctggc tcgcaagagg
     7741 ccgtccatgc ggaagccgta ttccggcgtc tggatttcgc gggcatagcc tttgctgacg
     7801 gtgctgatgg catcgctgta aacgatcccg cccttgagga cgctgaggtc tccaaggaac
     7861 tcgagccggt cgatggtgag cagggcgggg tccagcccga tctcggggag ggctgagggc
     7921 gcgaagatcc cttggtagcc gagattatgg atggtgagga gggttttgac gcccaggaag
     7981 gtcggatcgc cagcgagtgc cgtccggagg tagaccggcg cgagggcggc ctgccaatcg
     8041 tggcaatgca ggatttgcgg gcggaacaca tgccgggcgg atgccagcgc ggctttggcg
     8101 aggaccgcga agcggatgtg attatccggg aagtccccag cggcgtcgac gtaaacgccc
     8161 ttccggtcat agagggaggg gcattcgacg aagaaatacg ggacatcgcg ctcgaccgcc
     8221 atctggacgt tgaccgaata gtgcgcgagg ccgagccaga cgtccagttt ttcggccacg
     8281 cggcgggcgc cgtcgacccg cgcatcgcgg tagagaggga gcacgaccgc tacgtcttcg
     8341 ccgcgtgcct tgagggcggc cggcagcgcg ccgacgacgt ccgccagccc gccggtcttg
     8401 ataaacggcg tggcctcgga ggcgaccatc aggattttgg ccattcctct cgattgtcgc
     8461 cggtcggcat gggtcgtgac aacggatctt tgcagtgctg cacggcgtta taccgcgact
     8521 atgaagttcc gtgtggttct ggactacgat ccggaagccc ggagctactc ggcggtctgt
     8581 cctgaattgc cggggtgcgc ctcggccggc gaaacggaag aggaagcgaa acgcggtatc
     8641 gaggaagcga tccggctcta cttctcttcg gctgggattg agttgtcgcc gaccgcgaag
     8701 ctggtcgagg tcacagttgg atgactgcag aacgccgcgt ctagcggccg accaagtgat
     8761 ttcggttctc cgccgtcagg gttttgttct cgctggttcg gtggacagcc atcatgatgt
     8821 taacactctt gctggcggga gtcgccgccg cgcaggacgt gcagtacaac tttgatcagc
     8881 aggtcgattt ctcccgcttc aagacgtaca agtgggcaga gattccgggc ggccagaagc
     8941 tcgacgatct gttgtccagg catattcggg acgccttcga tgccggtctt gccaaaaaag
     9001 gtttgaccaa gaccgacgcc gacagcgcgg acttgtacgt tggctggcaa gtggcggtca
     9061 accaggagaa ggagcttacc gcttacggca ccggctgggg ctacggccct ggttggcgcg
     9121 gatcaggaat gggcactgcc aagacgtcaa cgatcctcat cggcacgctc gtgctcgata
     9181 tgtatgacac gcaaaacaag caactggtct ggcgctcatc cgccacgaaa acaatcgaca
     9241 ccaatgcgaa cccggaaaag cgctccaaga acctcgctaa ggctgtcgac aaaatgctga
     9301 agaattaccc gccaaaaaag tagccccagc gccgtcaggg tcgaaatcca accggcgatc
     9361 accggtccga cgcagtgccg gccgagggct ctgcgggcgg cccggcccat ttcaccagct
     9421 tcaggtccag aacgaaaatc gcgtatagaa ccggaaccag cagaagcgtt acgaaggttg
     9481 caatggtaag tccgccaatc tgggcgtagc acagcggctc ccagagcggc ccgccatggg
     9541 aggcgagcgg atacagcgca atcacggtcg cgccgaccgt gatcagaacc ggcctcagtc
     9601 gcatgatgcc cgcatcgagg agcgactcga cgaacggatg accttccgcg tggcgctctt
     9661 cgatg
//
LOCUS       DATIFQ010000374         2790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1082688, whole genome shotgun sequence.
ACCESSION   DATIFQ010000374 DATIFQ010000000
VERSION     DATIFQ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082688"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VLE22_18315"
     CDS             <1..1605
                     /locus_tag="VLE22_18315"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSB16411.1"
                     /translation="GIYDSVSLLLGDNPIIETVQVAPRIKSSEIIVETKLRNYGAARA
                     AELRHTLKAWKGGQQAASGAPERIELKAGEERTVRQTIRIPNARLWSPEEPFLYVLES
                     STGGDSASSRFGMREFRFDTATRRAWLNGKVYFLRGSNVTLHRFFEDPGCKRLPWTEA
                     WVRKLLAEIPKKMNWNSFRFCIGPVPDRWLEIADEAGLLIQNEYFIWTGHPEWRGVYS
                     RKWNGDFLVEEYKRWMRDNWNHPSVAIWDANNESFDPIFADKVIPAVRGLDLSGRPWE
                     NSYNIPVGPDDPVEHHPYLFSRTENDSDRPFRMAELEEMGGAPRGAGNIPSGHAAVVN
                     EYGWIWLNRDGTPTELTKKVYADLTGPNATAEERFAMNGYLLGGLTEYWRAFRHYAGV
                     LHFVYLTCSYPGVYTSDHFRNLETLELDPYFADYVGEAFKPLGIYIHFWQPELKAGAE
                     RTFPVMMVNDGARPVKGRLVLSLEAEGGGEAARAETAFELMANGQETYLVKLRVPAVS
                     GSHLLKASAVPGSGGPTVSRRKVTVQ"
     gene            1745..2395
                     /locus_tag="VLE22_18320"
     CDS             1745..2395
                     /locus_tag="VLE22_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16412.1"
                     /translation="MRFVCLIPCASFALLLSCAPRAVRTSGKPADLSQTVPKPTRGID
                     YIDLQPGWRLRVTTPLLKSGAYRLRLNEKQVDGNTVTLSAGEDFEGYETAFYAVEPRK
                     GGGVRIGFVSADMMKEGKSVPQVRPKELLFRLPPSTRMVRLIYLTRVSQFDHDMAVVA
                     ADETGALERLTQAVKTGQDACKSEDDSVCFWIPAGIALRPEMQTNVGGSKQWVPVQ"
     gene            2410..2784
                     /locus_tag="VLE22_18325"
     CDS             2410..2784
                     /locus_tag="VLE22_18325"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HSB16413.1"
                     /translation="MKTKNTTPTTQELEIMKVVWALGPATVRDVYEALRRRRKVAYTT
                     VMTMMNILEQKGYLKKKPGERAYVYHAAKPRKQVIRNMVEDFLGRVFDGSARPLLVHL
                     VEGRRLSADDLRDIERMMEEGE"
BASE COUNT          596 a          841 c          857 g          496 t
ORIGIN      
        1 ggcatctacg acagcgtctc gctgctcctc ggggacaacc cgatcattga gaccgtccag
       61 gtggctcccc ggatcaagtc atccgagatc atcgtcgaga cgaagctaag aaattacggc
      121 gccgcacggg ccgccgaact gagacacacg ctaaaagcct ggaagggcgg acagcaggca
      181 gcctcggggg cacccgagcg tatcgaactc aaagccggcg aggagaggac cgtccgtcag
      241 acgatccgca tcccgaacgc gcggctctgg tcgcccgagg agccgttttt gtacgtgctc
      301 gaaagctcga ccggcggcga cagcgcctcc agccggttcg gcatgcgcga gttccgcttc
      361 gacaccgcca cccgccgcgc ctggctcaac ggaaaagtct acttcctgcg cgggtcgaat
      421 gtcacgctgc atcgcttctt cgaggaccca ggctgcaagc ggcttccctg gaccgaagcg
      481 tgggtccgaa aactcctcgc cgagatcccg aagaagatga actggaacag cttccgcttc
      541 tgcatcggcc ctgtgcccga tcgctggctg gagatcgccg acgaggccgg cctgctcatt
      601 cagaacgagt acttcatctg gaccggccat ccggaatggc gcggcgttta cagccgcaag
      661 tggaacggcg actttctggt cgaggagtac aagcgctgga tgcgcgataa ctggaaccac
      721 ccgagcgtgg ctatctggga cgcgaataac gagtcattcg acccgatctt cgcggacaaa
      781 gtgatcccgg ccgtgcgcgg tctagacctc tccggacgcc cctgggaaaa cagctacaac
      841 attcccgttg gaccggacga ccccgtcgag caccacccgt atctcttcag ccgcacggaa
      901 aacgacagcg accggccgtt tcgcatggcc gaactcgagg agatgggagg cgcgccccga
      961 ggcgccggca acattccaag cggccacgcg gccgtggtca atgagtacgg ctggatttgg
     1021 ctcaatcgtg acggcacgcc caccgaactg acgaagaagg tctacgcgga tctcacgggc
     1081 cccaacgcca ccgccgaaga acgtttcgcc atgaacggct acctgctggg cgggttgacg
     1141 gagtactggc gcgctttccg gcattacgcc ggtgtgctcc attttgtcta tctcacgtgc
     1201 agctatcccg gcgtttacac ctcggaccac tttcgaaatc tggagacgct cgagcttgat
     1261 ccgtactttg cggactacgt cggtgaagcc ttcaaaccgc tcggcatcta catccatttc
     1321 tggcagccgg aacttaaggc cggcgccgaa cggaccttcc cggtgatgat ggtgaacgat
     1381 ggagcccggc ccgtgaaagg ccgcctggtc ctgtcgctcg aggccgaggg cgggggagag
     1441 gcggccaggg cggagacagc ctttgagctg atggccaacg gccaggagac ctacctcgtg
     1501 aaactgcgtg ttcccgccgt ctccgggagc caccttctga aggcttcggc ggtccccggc
     1561 agtggcggcc cgaccgtcag ccgccgcaaa gtgaccgtgc agtagcgtgc tgcgttgttt
     1621 tgcggcgaag gcggcagacg acaaacggcg atcgtctgcc ccacaacacg gagtggactt
     1681 gacaaacgaa tcccttcgta ctaaaaaatt agtatggaga cctgccggag gcaatgagac
     1741 gagaatgaga tttgtgtgcc tgatcccatg cgcttccttt gccctgttgt tgagttgtgc
     1801 cccgcgcgca gtgcgaacct ccggcaagcc agccgacctg tcgcagactg ttccgaaacc
     1861 aactcgtggt atcgattaca ttgacctgca acccggatgg cggttgcgcg tgactacacc
     1921 tttgctgaag tccggagcgt atcggctgcg gctgaatgag aaacaggtcg atgggaacac
     1981 cgtcacgttg tccgcggggg aggatttcga aggatacgag accgcattct acgcggtaga
     2041 accgcgaaag ggcggaggag tgcggatcgg ctttgtctcc gctgacatga tgaaagaagg
     2101 taagtcggtt ccgcaggtgc gtcctaaaga gctgctattc cggctgccgc ccagtacgag
     2161 aatggtgcgg ctcatctatc tcacgcgtgt cagccagttc gatcacgaca tggccgtggt
     2221 ggccgctgat gagaccggcg cacttgagag attaacgcag gcggtcaaga ccggccaaga
     2281 cgcctgcaag agcgaagatg acagcgtttg cttctggatc cctgctggaa ttgccctccg
     2341 gcccgagatg caaacaaacg taggcggctc aaagcagtgg gttcctgttc agtagaactt
     2401 ggctaagcga tgaaaacgaa gaacaccaca cctacaacgc aagaactcga gatcatgaaa
     2461 gtcgtctggg ccctcggccc ggccaccgtc cgcgacgttt atgaagccct ccgccggcgc
     2521 cggaaagtcg cctacacgac ggtcatgacg atgatgaaca tcctcgagca gaaaggttac
     2581 ctgaaaaaga aaccgggcga gcgggcttac gtctaccatg cagcgaagcc gcgaaaacag
     2641 gtgatccgga acatggtcga ggattttctt gggcgggtgt tcgacggctc cgcccgccct
     2701 ctgctggtcc atctcgtcga gggccgccgg ctgagcgcgg acgacctgcg tgacattgag
     2761 cgaatgatgg aagaaggcga gtgacgcgat
//
LOCUS       DATIFQ010000375         9977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_891351, whole genome shotgun sequence.
ACCESSION   DATIFQ010000375 DATIFQ010000000
VERSION     DATIFQ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9977
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891351"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VLE22_18330"
     CDS             complement(<1..552)
                     /locus_tag="VLE22_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16414.1"
                     /translation="MLRNGTIYDGSGGKPFRGDLAIRGDRIAAIGDVGKATGRIDLDL
                     KGLAVAPGFINMMSWANESLIEDGKSQSNIRQGVTLEVMGEGESMGPLNDSMKKELIG
                     RQGDIRYAVQWTTLGEYMMFLERRGISPNVASFIGAATPRIYVIGHAIRAPKPEELEK
                     MRQLVREAMREGALGIASSLIYPP"
     gene            783..1526
                     /locus_tag="VLE22_18335"
     CDS             783..1526
                     /locus_tag="VLE22_18335"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSB16415.1"
                     /translation="MFLNDWECCCTSCQARPASVFQGIEINRPKVAGGRCQSAVYPAG
                     SILFLQDEPARSALCIRSGRVKLSATAPDGRTVIVGIAAAGDILGTRALLSGRPHDMT
                     AEVIEETATCAILKDDFVALLRSNSEVALRVAERMGGEAAEAYRQLYAAVFKEPAERL
                     TELLLQLCGTHGEPTAEGIHLRTNLCQDEMAALVGVSRRTLNRSIAKLRRLGIIDCRR
                     KSIVIRDLDALRGWLSGQALGLGATRLAD"
     gene            1622..2872
                     /locus_tag="VLE22_18340"
     CDS             1622..2872
                     /locus_tag="VLE22_18340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HSB16416.1"
                     /translation="MHIFRAFSTRFRRICAVCAVLAGACVTLSRGAVPSALFQYNVLE
                     VMNSQPGQTPSVRFSVTNPTNGNAPYDITVHDSFKTVNGARLFIQIGWDTRDYHNTGS
                     QSETLNSPKNAAQPIPINALAFPQKHYIGGVWDGSFVVTASRPIPATAQGSGNAAMEG
                     RLVELVGGVWVQVAVRSAFKFFAITDPSPVPRRAVVDVNKCKNCHGSVAPPLVVHGNN
                     RAEEVMVCVGCHNPNATDIAYRTSGKETPIDFKNMIHAIHAGRMRHNPEVIIGFGGSV
                     NDFSYVDFPASLADCQRCHIPGAYSLPLGKNVLGTTVDTRSVLDAGGKVVDGDPTNDL
                     NITPTAAVCSACHDGNEARRHMVEKGASFGALQQSIDSGRVRERCATCHGAGKEEDVV
                     KVHAEEAHESSGEHEVHEEERDDH"
     gene            2942..3970
                     /locus_tag="VLE22_18345"
     CDS             2942..3970
                     /locus_tag="VLE22_18345"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB16417.1"
                     /translation="MTTEERNSARNLPPMPARREMLVGSAAMAAFLAGRSAVAGQTKE
                     PVRRKPSSRAYAMKKSINLWAFPYPSRWSLQECLQLAKDAGFDAVELNFDLESDLSIK
                     ATDEDIRGIRRTAEKTGIAISGVCSFLFWPYPMTANDPEKRKKSLEFANRMIEAAALL
                     GTENLLVVPGAVYAPWIENFDPVPIDVCDHRAREAVRSLIPKAEETGVYLNIENIFTS
                     GYLFSPQEMNTFVDSFGSDHVQIHFDTGNIMEYQFPEHWVPILGKRIRNIHFKEWDKR
                     THEFNLHTFRTLLDGTTNWPAVIEELNKLDYRGYLTFEYFHPFRHYPEALIWQTSDAL
                     DWMLGRKG"
     gene            complement(3984..5102)
                     /locus_tag="VLE22_18350"
     CDS             complement(3984..5102)
                     /locus_tag="VLE22_18350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSB16418.1"
                     /translation="MRSGIVALVMSSLFVPCLPGSAQFPRGITSRIEKVEKVTDKLKD
                     VELTPEEEVRLGEEISSRIRTKYGVVQDPAVHKYVSLVGTVVARKSGSTMPFHFIVLD
                     TDGVNAFAAPGGFIHITRGALALVKDESQLAGVLAHEVAHVTQQHTIRAIQKGKVVQM
                     AAKDRNVSSNPELFRRLTDEAYNIVFAGFGRADELDADTQGFGFAVGAGYAGGGLGMF
                     LATLKERNGSSSQRQGLFASHPEMDERKQKLDAMVQSRSGDPGATLAERYTGNVKFQP
                     VDLAQIAAVEAGAAGLAGETKKEEPKKKSRFSMASLRNPLGTTESSPQSAAVTGSGGS
                     RGVDKERLAKGGPNPAPVAVTLTEAEITDFRTTGNLKA"
     gene            complement(5139..7157)
                     /locus_tag="VLE22_18355"
     CDS             complement(5139..7157)
                     /locus_tag="VLE22_18355"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSB16419.1"
                     /translation="MTRAALRKTLAGLGIAAFAVCSALLLNQAPLLRTVEWKIYDLLI
                     RHTAGPAKARSDIVMVEIDDQSIERMAENGFGRFPWPRDTYAVLLRYLEKARPKAVAF
                     DLLLLEEDRGPEGASRDRELVEATRRMGNVIHSVAVSDSYVSAPPSAPGGYLLGREVE
                     EHRSVKLPFGELGSASRALGHTFLVLDEDGPVRRAVPFVRQKDNYYPSLAIATAMAVL
                     GIEPSRVRLDRDGLRLGDLSIPLIDLDVEYVEKIRARHMLVNYRAPAFRDAARTERAY
                     RTYKFWDLLLSQAQIEDGRTPAIDPQSFRDKIVFVGTTASGLHDVFQTPFGETGKFPG
                     MQIHASVVDDILSRSFLRPAPRGWAVALLVVFATLVGVAGVALNFWWASLVALVIASG
                     GAAAVVLLFRQGAWLPSVPAITGLLVAQFASVAYSYFVEGRSKRQIRALFSRYVSPEV
                     VKELIEDPSKARLGGERREMTVLFSDIRGFTTFSESRDPEDVIRQLNEYFSRMVELLF
                     EHKGTLDKFVGDMIMGLFNAPVREPDHADNAVRMGLAMLGELKVLNERWGKEGRPALD
                     IGVGINTGDMIVGNVGSERTLSYTVIGDNVNLGSRLESLNKEFGSHIIISEFTRNRLR
                     GDYFIRPLGKVKVKGKNREVAIFEVCVSEEELRAKEARAAEPSSAATH"
     gene            7418..9274
                     /locus_tag="VLE22_18360"
     CDS             7418..9274
                     /locus_tag="VLE22_18360"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSB16420.1"
                     /translation="MKLPLLRILLLPALALPLASAPQQPRTIEELQKEINNLLVQHGV
                     PGVGIVMVTPDRVLWAGGAGKADIAEGRPVTADTLFRCGSISKSFVALALMKLQEEGR
                     IDLDAKVKDLAPEIDIPNPWEATHPVRVAHLLEHTAGFDDWRWAGNLTEDATEIPLRG
                     VMDLFPGRQRVRWLPGTRMVYSNHGYVVAGYLIEKITGQRFDDCIRSAVLNPLGMQRS
                     CFRPTDANRTLLARGYNYRPLEPLPYRFGLQRPAGELLISPAEMGSFLQMWLNRGKVG
                     GIQLFRTESLARVEEPRTSLAARMGLKSGYGLGNHPMNAPGLRRRGHGGQIEGFLAFY
                     VYMPEVRLAYAVFMNTWSPSLASIDQLVGEYLTNGLPKVQSPQEAPSADAELRRQEGV
                     YLPADFRVHAGTWPQLLQRACRLSVEGGALYKASLFGKGREQFTSAFPGAFRTKDELE
                     PSILFSRSDDGKWVMTGSRGYFERVPTWRPYACAVGLLLAFLLMLSAVLHGLVWIPAK
                     LLGRNVKHVVVRAVPLCSVIVFVALFVLMLRAADDPTASRHLDLPTIGYCLLSWLFPV
                     LSFWALLLARRSFEYELRPAVRIHALLVSIACCGMATTLAYWGLLALRPFVS"
     gene            complement(9677..>9977)
                     /locus_tag="VLE22_18365"
     CDS             complement(9677..>9977)
                     /locus_tag="VLE22_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16421.1"
                     /translation="GPFNGCLAGQGVRWDTVAPLASTTFKCTGAAGEALKTAFTGDST
                     VVLLADFYRQGDGNDESFTAQMIVSGSDLAPEIPGVQNVWIQGIGCGEAVANFNP"
BASE COUNT         1862 a         3072 c         3136 g         1907 t
ORIGIN      
        1 cggcggatag atcagcgagg aggcaatgcc cagcgcgcct tcccgcatcg cctcgcgcac
       61 gagttggcgc atcttctcga gctcttccgg cttgggcgcc cgaattgcgt gcccgatcac
      121 gtaaatccgc ggcgttgccg caccgatgaa cgaggcgacg ttgggcgaga tgccgcgccg
      181 ctccaggaac atcatgtact cgcccagcgt cgtccactgg accgcgtatc tgatgtcccc
      241 ctggcggccg atcagttcct tcttcatcga gtcgttgagc ggccccatcg actcgccctc
      301 gcccatcact tcgagcgtga cgccttgccg tatgttgctc tgcgactttc cgtcctcgat
      361 gagcgattcg ttggcccagc tcatcatgtt gatgaagccg ggcgccactg ccaggccctt
      421 caggtcgagg tcgatccttc cggttgcctt gccgacgtcg ccgatagcgg cgatccggtc
      481 tccgcgaatc gccagatctc ctcggaatgg cttcccgccg ctgccgtcgt agatcgtgcc
      541 gttccgcagc acagcatcgt attgaatagg ccacgaacag cccgagaaaa taagagatcc
      601 caagcacaga acaacactga tcgcccgcat ggtaacgagt atagagccat ccggccggta
      661 gaaattacac gccttactga acgcgccacg gaaaaactcc aatgcaaacg ccgtcttccg
      721 gtttggcgcg ataagtgcga ggaaggaccc cgtctctagc ccaacaactc agaggaactg
      781 ggatgtttct caatgactgg gaatgttgtt gcacatcctg ccaggcacgt ccagccagtg
      841 ttttccaagg aatcgagatt aatcgtccga aggtcgccgg cggccgttgc cagtcagcgg
      901 tctatccggc gggatcgatt ctgttcctgc aagacgaacc ggctcgctcg gcactgtgta
      961 ttcggtcggg acgggtgaaa ttgtcggcta ccgccccgga cggccggacg gtgattgtcg
     1021 gaatagccgc tgcgggcgac atcctcggca cgcgagcgct actctctggc cgtcctcacg
     1081 acatgacagc agaagtgatt gaggaaacag caacttgcgc cattctgaag gacgatttcg
     1141 tggcactgct tcgctctaac agcgaggtcg cgcttagggt ggccgagagg atgggcggtg
     1201 aggccgccga ggcgtatcgc cagctttacg ccgctgtatt caaggaaccc gcggagcgac
     1261 tgaccgagtt gctgcttcaa ctttgcggga ctcacggcga gccgaccgcc gagggaatcc
     1321 accttcgcac caatctgtgc caggacgaaa tggctgcgct ggtcggggtt tcgcgcagga
     1381 ctttgaaccg ttcgatcgcg aagctgcggc gcctcggaat catagactgc cgacgcaagt
     1441 ccattgtgat cagggacttg gatgcccttc gcgggtggtt gtccggccag gctctcgggc
     1501 ttggcgccac ccgcctcgcc gactgacccc ccgaatgtgc cagatggcct cgaacggccg
     1561 gcgcccttcg tgttactcaa tacgaaacgc tgttcaaaaa acgatcacga ggaggcagtt
     1621 tatgcacatt tttcgcgcat tttcgacgcg ttttcggcgc atttgcgccg tttgtgctgt
     1681 gcttgccggc gcatgtgtca ctttgagccg aggcgccgtc ccttcggctc tgtttcagta
     1741 caacgttctg gaggtgatga actcgcaacc ggggcagacg ccctcggtgc ggttttcggt
     1801 caccaacccg acgaacggga acgctccgta cgacatcacg gtgcacgatt cgttcaagac
     1861 ggtgaacggc gcacgcctgt tcattcagat cggctgggac acgcgtgact atcacaacac
     1921 cggcagccag agcgagacac tcaacagtcc gaagaacgcc gcccagccga tcccgatcaa
     1981 cgcattggcg tttccgcaga agcactacat aggcggggtt tgggacggtt cttttgtcgt
     2041 gacagcctcg cggccgattc cggcgacggc gcagggatcg gggaacgcgg ccatggaggg
     2101 gcgcctcgtc gagttggttg gcggtgtgtg ggtccaggtc gcagtgcgca gcgcgttcaa
     2161 gttcttcgcc atcacggacc cttcgcctgt tccgaggcgg gcggtcgtgg acgtgaacaa
     2221 gtgtaagaac tgccacggtt ccgttgcgcc tccgctggtt gtgcacggga ataaccgggc
     2281 cgaagaggtc atggtctgcg tggggtgcca caacccgaat gcgacggaca tcgcctacag
     2341 aacctccggc aaggaaacac cgatcgattt caagaacatg atccacgcaa tccacgccgg
     2401 caggatgcgc cacaacccgg aggtgatcat cggcttcgga ggttcggtga acgacttcag
     2461 ctacgtggac tttccggcga gcctggcgga ctgccagagg tgccacattc cgggcgcata
     2521 cagcttgcca ctcggcaaga acgtgcttgg gacgacagtt gacacgcgaa gtgtactcga
     2581 tgccggcggc aaggtggtgg acggcgatcc gacgaacgac ttaaacatca cgccgaccgc
     2641 cgcagtctgc tcggcttgcc acgacggcaa cgaggcccgc aggcacatgg tggagaaggg
     2701 cgcctcattc ggtgccctgc aacagagtat cgactccgga agggtccgcg agcgctgtgc
     2761 gacctgtcac ggtgcaggca aggaagagga tgtcgtcaag gtccacgccg aagaagcgca
     2821 cgagagttca ggcgagcacg aggtccacga ggaagagcgc gacgaccact gacgcagcgt
     2881 ctctcttgac ggagacagcg cgtccgtgaa aggctgaaga atcccctggg attcttcagc
     2941 catgacaacc gaagaacgta actccgcacg gaaccttccc ccgatgccgg cgcggcggga
     3001 aatgctggtg ggctccgcgg cgatggccgc gtttctcgcc ggacgctcgg cggtcgccgg
     3061 gcagacaaag gaacccgtcc gccgcaagcc ctcgtcgcgc gcctacgcga tgaagaagtc
     3121 gatcaacctg tgggccttcc cgtacccgag ccgctggtcg ctacaagagt gcctgcaact
     3181 ggcgaaggac gcaggtttcg atgcggtcga gctgaacttc gacctcgaga gcgatctctc
     3241 gatcaaggca accgacgaag acattcgagg aatccgccgg acggcggaga agaccgggat
     3301 cgccatcagc ggcgtctgtt cgtttctctt ctggccctac ccgatgacgg ccaacgatcc
     3361 ggagaagcgg aaaaagagcc tggagtttgc gaaccggatg atcgaagctg cggcgctgct
     3421 tggaacggag aacctgcttg tggttccggg cgccgtgtac gcgccctgga tcgagaactt
     3481 cgatccggtg ccgatcgacg tctgcgacca ccgggcccgg gaggctgtgc ggtcgctcat
     3541 tccgaaggcg gaagagaccg gagtctatct gaacatcgag aacatcttca ccagcgggta
     3601 tctgttcagc cctcaggaga tgaacacttt tgtcgacagc tttggcagcg accacgtgca
     3661 gatccacttc gacaccggca acatcatgga gtaccagttt ccggaacact gggtcccgat
     3721 cctggggaag aggattcgga acatccactt caaggagtgg gacaagcgga cgcacgaatt
     3781 caacctgcac acgttccgaa cgctactcga cggcacgacc aactggccgg cggtgatcga
     3841 agagttgaac aaactcgact atcgcggcta tctcacgttc gagtacttcc accccttccg
     3901 ccactatccc gaggcgttga tctggcagac ctccgacgcc ctggactgga tgctggggcg
     3961 gaaggggtag aagctggttg tcgctaagct ttcaggttgc cggtggtgcg gaaatcggtg
     4021 atctccgctt cggtgagcgt cacggcgacg ggcgccgggt tcgggccgcc ctttgccagg
     4081 cgctccttgt ccactcctcg cgatcctccc gagccggtca cggccgcgct ctgggggctc
     4141 gactcggtcg tgccgagtgg gttccgaaga ctcgccatac tgaaacggct cttcttcttc
     4201 ggctcttcct tcttcgtctc gcccgccagg ccggcggccc ccgcttcgac ggcggcgatt
     4261 tgcgcgaggt cgacgggctg gaacttcacg ttgcccgtat accgctccgc cagcgtcgcg
     4321 ccggggtctc ccgaccggct ttgcaccatg gcgtcgagtt tctgcttacg ctcatccatc
     4381 tcggggtgcg aagcgaacag gccttgccgc tggctcgaag agccgttcct ctctttcagg
     4441 gtggccagga acatccccaa tccaccgccg gcgtaacctg ccccgacggc aaacccgaat
     4501 ccttgcgtgt ccgcatccag ttcatccgcg cgtccgaatc cggcgaacac gatgttgtat
     4561 gcttcatccg tcaggcggcg gaagagctcg gggttggacg acacgtttcg gtccttcgcc
     4621 gccatctgca ccaccttccc cttctggatc gcgcggatcg tgtgctgctg cgtcacgtga
     4681 gcgacctcat gagcgagcac gcccgcgagt tgcgactcgt ccttaaccaa ggccagcgcg
     4741 ccgcgggtga tgtggatgaa tccccccggc gccgcgaacg cgttcacgcc gtcggtgtcc
     4801 aacacgatga agtggaacgg catggtcgag ccgcttttcc gcgcgaccac cgtgccgacc
     4861 agagacacat acttgtgcac cgccgggtcc tgcaccacgc cgtatttcgt acggatgcgg
     4921 gagctgatct cctcgcccag gcggacctcc tcttccggcg tcagttcgac gtccttcagc
     4981 ttgtcggtga ccttttccac cttttcgatc cgcgacgtga tcccgcgcgg gaactgcgcg
     5041 ctgcccggca ggcacggcac gaaaagactc gacatgacga gcgccactat cccagatctc
     5101 atagcctcct ccttcacaaa tcgcttctca aaacatcgtc agtgcgttgc cgccgaggac
     5161 ggctcggcgg ccctggcttc ctttgccctc agctcttctt cggacacgca tacctcgaag
     5221 attgccacct cgcggttctt tccttttact ttgactttgc ccagcggccg tatgaagtag
     5281 tcccctcgca gccggtttcg ggtgaattcg ctgatgatga tatgactgcc gaattccttg
     5341 ttcaaagact ctagcctgga gccgagattg acgttgtctc cgatgacggt gtagctcaga
     5401 gtacgctccg agcccacgtt gccgacgatc atgtcgccgg tgttgatgcc gacgccgata
     5461 tccaatgcgg ggcggccttc ctttccccac cgttcgttga gcactttcag ctcacccagc
     5521 atcgccagcc ccatgcgaac cgcattgtcg gcatgatccg gttcccggac gggcgcgttg
     5581 aacagcccca tgatcatgtc gccgacgaac ttgtcgagcg tccccttgtg ctcgaacagc
     5641 agctcgacca tgcgcgaaaa gtattcgttc aactggcgga tgacgtcctc cgggtcgcgc
     5701 gattcggaga acgtggtgaa cccccggatg tccgagaaca ggacagtcat ttcgcgccgc
     5761 tcgccgccaa ggcgcgcttt ggaaggatcc tcgatcagtt ccttgacgac ttccggggag
     5821 acgtagcggg agaagagtgc gcggatctgc cgcttggacc gtccctcgac gaaatagctg
     5881 taggcgacac tcgcgaactg ggccaccagc aggccggtga tagcgggcac ggacggaagc
     5941 cacgctccct gccggaacag gaggactacc gccgccgcgc cgccggaggc gatcacaagt
     6001 gctaccaggc tcgcccacca gaaattgaga gcgacgccgg cgacgccgac cagcgtcgcg
     6061 aaaacgacaa gcaaagcaac cgcccaaccg cggggcgccg gacgcaggaa ggaacggctc
     6121 aggatgtcat ccacgactga agcgtggatc tgcatgccgg ggaacttgcc ggtctcgccg
     6181 aacggggtct ggaagacgtc gtggaggccg gacgccgtgg tcccgacgaa aacgatcttg
     6241 tcgcggaacg attgcggatc gatcgccggc gtccgcccgt cttcgatctg ggcttgggag
     6301 agcagcagat cccagaactt gtaggttcgg taagcgcgtt cggtccgtgc ggcgtcgcgg
     6361 aacgcgggcg cgcggtagtt cacgagcatg tggcgggcgc ggatcttctc gacatactcg
     6421 acgtccaggt cgatgagcgg gatcgagagg tcgccgaggc gcaagccgtc gcggtcgagc
     6481 cggacgcgag agggttcgat cccgagcacg gccatggcgg tggcgattgc aagcgacggg
     6541 tagtagttgt ccttctgccg cacgaaggga acagcgcggc ggaccggtcc gtcttcatcg
     6601 agcacgagga acgtgtggcc aagggcgcgg cttgcggagc caagctcgcc gaagggaagc
     6661 ttgacgctgc ggtgttcctc cacttcgcgg ccgagcaggt agccgccggg cgctgagggc
     6721 ggcgcggaga cgtacgagtc actgacggcg acggagtgaa tcacgttgcc catgcgccgg
     6781 gtggcttcga cgagctcccg gtcgcgcgag gcgccttcgg gtccgcgatc ttcttcgagg
     6841 aggagcaggt cgaaggcaac cgccttgggg cgcgctttct ccaggtagcg gagcaggacg
     6901 gcgtaggtgt cgcggggcca gggaaaacgg ccgaagccgt tttcggccat gcgctcgatg
     6961 ctctgatcgt caatttcgac catgacgatg tcggagcgag ccttggcggg gccggcggtg
     7021 tggcggatga gaaggtcgta gatcttccac tcgacggtgc ggaggagcgg cgcctggttg
     7081 aggagcagtg cggagcacac agcgaaggca gcgatcccga gtcccgcgag tgtcttgcgc
     7141 aaggcggcgc gtgtcacggg cggattgcct cccggaactg gtacgcgact ccccagtgga
     7201 acagtggttt cgacaagacg gccctcagca acgacgatgt ggaacccagg tggatacgag
     7261 tatacaacag ggggcggtgt gggagatgtt cacggggtag cggatcgcca ccaggcatcc
     7321 gggcattggg agacagactg ctcggccctc ggaacctgag cccttccgca ggtgtctaat
     7381 tcggatgaaa ttctccggtt tcacggagct tgtgaccatg aaattgccgc tgctcaggat
     7441 cttgcttttg ccggctttag cactgccgct ggcgagtgcg cctcagcaac ctcgcacgat
     7501 cgaggagcta cagaaagaga tcaacaacct cctcgttcaa catggcgttc cgggcgtcgg
     7561 tattgtcatg gtcacgccag accgcgtgtt gtgggccggc ggagccggca aagcggacat
     7621 tgccgagggg aggcccgtca ccgcggacac cctgttccgt tgcggctcaa tatccaagag
     7681 tttcgtcgcc ttggctctga tgaagttgca ggaagaaggc aggatcgatc tcgacgcgaa
     7741 agtgaaggat ctcgcgccag agatcgacat cccgaacccc tgggaagcca cgcatccggt
     7801 ccgagttgcc cacttgctgg agcacacggc tggctttgac gattggcgtt gggccggaaa
     7861 cctcaccgaa gacgctacgg aaatcccttt gcggggcgtg atggacctct tccccgggcg
     7921 gcagcgggta cgctggctgc caggcactcg tatggtttac tcgaaccacg gttatgtcgt
     7981 ggccgggtat ctcatagaga aaatcacggg gcagcgcttc gacgactgca tccggagcgc
     8041 cgtcctcaac ccgttgggga tgcaacggtc ctgcttcagg ccgacagacg ccaaccgaac
     8101 attgttggcg cgaggctaca attatcggcc gctcgaacca ttaccgtacc ggttcgggtt
     8161 acagcggccc gcaggggaac tcctgatttc gcccgcggaa atgggttcct tcctgcagat
     8221 gtggctcaac cgcgggaagg tgggtggcat ccagttattc cggacggaat cgctcgcgcg
     8281 tgtagaagag ccgcggacga gtttggccgc acgcatggga ctgaagtcgg ggtacggcct
     8341 tggcaaccac cccatgaacg ctccaggatt gaggcggcgt ggccatggcg gccagattga
     8401 aggcttcctg gcattttacg tctacatgcc ggaggttcgg cttgcttatg cagtgttcat
     8461 gaacacgtgg tcgccctctc tggcgagcat cgaccagctc gtcggcgaat accttacgaa
     8521 tggcttgccc aaggtgcaat caccccagga ggctccaagt gccgacgcgg aactgcgccg
     8581 ccaggaaggc gtctacctgc ccgccgactt ccgggtgcat gccggtacgt ggccccagtt
     8641 gctgcagagg gcatgccgct tgagcgtgga gggcggagcg ttgtacaagg cgagcttgtt
     8701 cggaaaaggg cgggaacaat tcacctcagc gttcccaggc gcctttcgca cgaaggatga
     8761 gcttgagccg agcatcctct tcagccggtc cgacgatggt aaatgggtga tgaccggttc
     8821 gcgcggttac ttcgagcggg tgccgacctg gaggccttac gcttgcgccg tcggactgct
     8881 cttggccttt ctcctcatgc tgagcgctgt gctgcacggc ctcgtatgga ttcccgcaaa
     8941 gctcctcggc cgcaacgtca agcacgtcgt ggtgcgcgcc gtgccgctat gctccgtcat
     9001 tgtgttcgtt gccctcttcg tactgatgct ccgtgcggcg gacgatccaa ccgcgtcgag
     9061 acaccttgac ctcccgacta ttgggtattg cctcctgtca tggctgttcc ccgtgctttc
     9121 tttctgggcc ctgttgctag cccgacgctc gttcgagtac gagctcagac cggccgttcg
     9181 gatccacgct ctgctggttt cgatcgcgtg ctgcggaatg gccacgacgc tcgcgtactg
     9241 gggcctgctc gcgctccgcc cgttcgtttc ttgaaggcga aggaatcggc tagcgacagg
     9301 cgtccgcttt cttcggtcgg aaactgcctg ccggcagggg agccggcacg gcggcgagga
     9361 gggcgcccat cctgtaggtg cgaatccgtc agctcactcg gcccacttga gggaggcccg
     9421 tggaggcccc agcggcgggc tttggagcgg ccgtcgtgga cacagacgac gcctatgcta
     9481 acgcggcgac caagcagggc tgggcctgtc aggtgcgagc tccttcgcgc ggcgcgaagg
     9541 cctcgcgcgc gctttcgaaa gcgatattgc gccgccccgg acccggccta agctgatagg
     9601 ccggtccgag gcaggttggc attgatatcc gcagtgcaac acgtaggtta cgacactggt
     9661 gtcgcgtgca agccggttag ggattgaagt tagctaccgc ttcaccgcag ccgattccct
     9721 ggatccagac gttttggacg ccaggtatct ccggtgcgag gtccgatcca gatacaatca
     9781 tctgcgccgt gaaagactca tcattaccgt cgccttgccg gtagaagtcg gcgagaagga
     9841 ctaccgtact atcgcccgtg aaggcggtct ttagagcctc accggcggcg ccggtacatt
     9901 tgaatgtagt cgaggcaagc ggtgcgacag tgtcccagcg cactccctgg cctgcaaggc
     9961 acccgttgaa tggtccc
//
LOCUS       DATIFQ010000376         2226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_700707, whole genome shotgun sequence.
ACCESSION   DATIFQ010000376 DATIFQ010000000
VERSION     DATIFQ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700707"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VLE22_18370"
     CDS             <1..206
                     /locus_tag="VLE22_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16422.1"
                     /translation="MSRTRYVPAFYLASIYYSMGDVARALQLGWKAVGERCDYLMYLR
                     VEPRAGKLAANPEFIRLVAALHR"
     gene            203..1120
                     /locus_tag="VLE22_18375"
     CDS             203..1120
                     /locus_tag="VLE22_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16423.1"
                     /translation="MSPPFRLLVLACGFSGMCWAARCTTVLDPLALDAYEAYVIAAEE
                     AASARFAAGELAWVPHSASAEASARLDSGRVIRQKLSDAAVNQRIAGQNAAIVHWIGA
                     VHIREVDLADLRTVLEDYPSYDRVYQPIFVGFRAERGGSGPDSGYDATFWLQHKFAFA
                     SLFRQHYAFRMKARIEYSHTDVSSGPALLVHMRSSEVRESDSGVPGRNDLMELYHDHG
                     IIWAVNAYWRARRREGGVYFEFETITLARSVQEFACKIGFIPVPRSMAAAAMDSLPAE
                     ALQVMLEGAKAECERRAAARAASSPLLRQ"
     gene            complement(1389..2024)
                     /locus_tag="VLE22_18380"
     CDS             complement(1389..2024)
                     /locus_tag="VLE22_18380"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB16424.1"
                     /translation="MSRLCCLFLVFIAVPCGGQRIGFGVKGGVRPTNDIVGSATSESK
                     RYVAGPMLDVSLPLGLGIEIDALYSRFGYRTATGGFWGSFSSVRRANVWEFPILAKYR
                     LPFPLIKPYVSGGYALRRVTGSYNSEGYTVDLSTGNRQWYTQHGSWEPEAGGGAVIGG
                     GVELGGRHFRIAPEVRYTRWSNDIINAYGSQGYRVNSALNQTQILLGLSWR"
BASE COUNT          414 a          704 c          687 g          421 t
ORIGIN      
        1 agatgtcgcg cacgcggtat gttccggcat tctaccttgc gtcgatctac tactcgatgg
       61 gtgacgttgc gagggccctt cagttggggt ggaaggcggt cggcgagcgc tgtgattacc
      121 tgatgtatct gcgcgtcgag ccccgcgctg gcaagctggc cgccaatcca gagttcatcc
      181 ggctagtggc cgctctccat cgatgagccc tcctttcaga ttgctcgtgc tggcctgcgg
      241 gttcagcgga atgtgctggg cagcgcggtg tactacggtt ctggaccctc ttgccctgga
      301 tgcatacgaa gcatacgtga ttgccgccga agaggcggcg tctgcccggt tcgccgcggg
      361 ggaactcgcc tgggtgcctc attccgcatc ggccgaggcg agcgccagac ttgattcggg
      421 cagagtgatc cggcaaaagc tcagcgacgc cgccgtcaat cagcgcatcg ccgggcagaa
      481 cgcggcgatc gtccactgga tcggggcggt gcacattcgc gaggtggacc tcgcggatct
      541 gagaacggtg ctcgaggatt accccagcta cgatcgtgtc taccagccga ttttcgtcgg
      601 gtttcgcgcc gagcgcggcg gaagcggtcc cgactccgga tacgacgcga cattctggct
      661 tcagcacaag tttgccttcg cttcgctgtt tcgacaacac tacgcgttcc gcatgaaagc
      721 gcgcatcgag tactcccaca cggacgtctc cagcggtcct gcgctgcttg tgcacatgcg
      781 gtccagcgag gttcgggaat ccgacagtgg cgtccccgga aggaacgacc tcatggagct
      841 ttaccacgac cacgggatca tctgggcggt caacgcgtac tggcgcgcgc gccggcggga
      901 aggcggcgtc tacttcgagt tcgaaaccat cacgctggcc cggtccgtcc aggagttcgc
      961 ctgcaagata ggcttcattc ctgtcccacg ctcaatggcg gcggcggcga tggattcgct
     1021 gccggcagag gcactgcaag taatgttaga gggcgcgaaa gcggagtgcg agcgtcgggc
     1081 cgcggcacgc gccgccagct cacctcttct tcgccagtaa atgccccacc ggcatcctca
     1141 ggtgaaagaa atcagtggtc tcctcggatt ccgtgtactg gaggacctcg aagtccggaa
     1201 acaacgacgg tagctcgccg cgttcgaggt cacccggaac gcgcggatgc cgttccttgg
     1261 gccgaacttc gacaacttac tgctggcggt ggcacagcct tcaggctgtg tgcctgtttc
     1321 tcagcactct tttagacaat ggaggcccgc ctccggttcc ctatctgtgt gcagtgtgcg
     1381 aacgcccgtt accgccaact caggccgagc aggatctgtg tctgattgag ggcggagttg
     1441 acgcgatagc cctgactacc atacgcgttg atgatgtcgt tactccagcg cgtgtagcgc
     1501 acttccggtg cgattctgaa atggcggccg ccaagctcga cgccgccccc gatcaccgct
     1561 ccgccgccgg cttcaggttc ccacgagcca tgctgcgtgt accactgccg gttgccagta
     1621 ctcaaatcga cggtgtagcc ctccgagttg tacgatccgg tgacgcgccg gagcgcatag
     1681 ccgcccgata catagggctt aatcagcgga aagggtagac ggtacttggc cagtattggg
     1741 aactcccaca cgttcgcacg cctgacggag ctgaagctgc cccaaaatcc gccggttgca
     1801 gtccggtaac cgaagcgact gtaaagcgca tcgatttcaa tcccgagacc caacggcagg
     1861 ctcacgtcga gcattggacc ggcgacatac cgcttcgact cagacgtagc ggagcccaca
     1921 atgtcattgg tcggccgcac gccacccttc acgccaaagc cgatgcgctg ccctccacac
     1981 ggaacggcga tgaatacgag gaacaggcaa cataaccgag acacagaaca cctcgaaggg
     2041 agtggaggac gcgataattc cagtttaccg ttcggggccg gtgaccgggg cttccgaacc
     2101 cgcttccggt tccgtggccg cctcagtgtg gggcaggacc ccggcctgcg gcggcctctc
     2161 aggccgcctg gccggagtcg ccactcagaa gcccgaccct gcgcagaatc ccctcgaaac
     2221 gggggt
//
LOCUS       DATIFQ010000377         2842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2231580, whole genome shotgun sequence.
ACCESSION   DATIFQ010000377 DATIFQ010000000
VERSION     DATIFQ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231580"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VLE22_18385"
     CDS             <1..1372
                     /locus_tag="VLE22_18385"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HSB16425.1"
                     /translation="KAAEAYNDPGRFTAFIGYEWTSNTGGNNLHRNVIFRENGDKARL
                     VEPYTTLKPLGSDNPEDLWKWMAATEEKTGSEVLAIAHNGNLSNGRMFPMVEAFGKKI
                     DKKYAETRVKWERLYETTQTKGTGEAHPYLSPNDEFANFELWDKGNLDGSAAKKKDML
                     EYEYARSAFKNGLLLEAKLGVNPYKFGLIGSSDAHTGLSAMEEENFFGKTAPQEPSPE
                     RMTKAFFKNPKTGITVMDWEVAASGYAGVWAIENTRGAIFDAMQRRETYATTGPRMIV
                     RFFGGWDYEPADANNRLPAAIGYAKGVPMGGDIKNAPKGKSPTFIAAALKDPIGANLD
                     RIQIIKGWVDKNGKLQERIYDVAVSGGRKIEADGRCKTAVGSTVDVENATWTNTIGDP
                     ELIAVWKDPDFDSAQRAFYYVRVLEIPTPRWTAYDAKRFGTKPLPGTTMTLQERAYTS
                     PIWYTP"
     gene            1524..>2842
                     /locus_tag="VLE22_18390"
     CDS             1524..>2842
                     /locus_tag="VLE22_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16426.1"
                     /translation="MSSMANDAEKKRLETCRSGARKIPWKKWGPYLSERQWGTVREDY
                     SEAGDAWNFFTHDHARSRAYRWGEDGIAGISDDKQRLCFSLALWNGKDPILKERLFGL
                     TNSEGNHGEDVKEYYFYLDSTPTHSYMKYLYKYPQAAYPYADLVETNRRRTRNDMEYE
                     LLDTGVFKDDRYYDVFVEYAKAGPEDILIKITAHNRGPDAAELHLLPTLWYRNDWASW
                     ITESNRAPEKPNLKQIKTAAGTSAVAATHRLLGEFILSCEGEVPLLFTDNTTNNARLF
                     PGQPNAGPYVKDGINNCVVQGDQAAVNPEKHGTKVAAHYRMTIASGQSATVRLRLTAR
                     GSAGQSTTTKSAAVPFGPAFDATLTARLQEADEFYRSVTPPSVSPDAANVMRQAIAGM
                     LWSKQFFFFDGDSWLEEHHSNPLHSGYRNSRNSEWFHMLNEDVISMP"
BASE COUNT          662 a          846 c          852 g          482 t
ORIGIN      
        1 caaggcggct gaggcataca acgacccggg caggttcacc gctttcatcg gctacgagtg
       61 gacctcgaac accggcggca acaacctgca tcgcaatgtc atcttccgcg agaatggcga
      121 caaagctcgc ctcgtcgagc cctatacaac gctgaaaccg ctcggcagcg acaaccctga
      181 ggacctgtgg aaatggatgg cggccacgga agagaagacc ggcagcgaag tgctcgccat
      241 cgcccacaac ggcaatctga gcaacgggcg catgtttccc atggtagagg ccttcggcaa
      301 aaagattgat aagaaatatg cagaaacgcg cgtcaagtgg gagcggctct atgagaccac
      361 ccagaccaag ggtaccggcg aggcgcatcc gtatctctcg ccgaatgatg agttcgccaa
      421 tttcgaactt tgggacaagg gcaaccttga cggtagcgcg gcgaaaaaga aagatatgct
      481 cgaatacgag tatgctcgtt cggcattcaa gaacgggctt ttgctcgaag caaaactggg
      541 agtgaaccca tacaagttcg gtctcatcgg aagcagcgac gcgcataccg gcctctccgc
      601 tatggaggaa gagaattttt tcggcaagac agcgccgcag gagccaagcc ccgagcgcat
      661 gaccaaagcc ttcttcaaga atcccaagac tggcataacg gtaatggact gggaggtcgc
      721 tgcttccggg tatgcaggcg tgtgggcgat agagaacaca cgcggggcga tcttcgacgc
      781 catgcagcgc cgcgagacct acgccacaac cggcccgcgt atgatcgtgc gcttcttcgg
      841 cggctgggac tacgagccgg ctgacgcgaa caaccgtctg cctgctgcca tcggctacgc
      901 caagggcgtg cccatgggtg gcgatattaa gaacgcgccg aagggcaagt cgccgacgtt
      961 catagccgcg gcgttgaagg atcccatcgg cgcgaacctc gaccgcattc agattatcaa
     1021 gggctgggtc gacaagaacg gcaagctgca ggagcgaatc tacgacgtgg ccgtgtctgg
     1081 cggacgcaag atcgaagcgg acggccgctg caagacagcc gtcggaagca cggttgacgt
     1141 ggagaatgcg acatggacca acaccatcgg cgacccggaa ctgatcgctg tttggaaaga
     1201 cccggacttc gactcggcgc aacgggcgtt ctactacgtt cgcgtcctcg aaattcccac
     1261 gccgcgctgg accgcgtacg acgccaaacg ttttggcacg aagccgttgc cgggaacgac
     1321 gatgaccttg caggagcgcg cctatacgtc gccgatctgg tatacgcctt aacacaggca
     1381 ggctggaatc aatgacgcgt ttccggcgat gacgagacaa ttcgcgtgaa tgccgcgtcg
     1441 aaactcctgg gattgctcgt cgtggcgggt tggcctcttg cgggctgcgt actgtcaagt
     1501 tcaattgcat aaaggagatg aacatgtcta gcatggccaa cgatgcggaa aagaaacggc
     1561 tcgaaacctg ccgcagtggc gcccgcaaga ttccgtggaa gaagtggggg ccgtatctgt
     1621 ccgagcgcca gtggggtacg gtccgcgagg actacagcga agcgggagac gcctggaatt
     1681 tcttcaccca cgatcatgcc cgctcgcgcg cctaccgctg gggcgaggac ggcatcgccg
     1741 gaatttcgga tgacaagcag cgcctctgct tcagcctggc cctgtggaac ggtaaggacc
     1801 cgatcctcaa ggaacggctc ttcggcctca ccaacagcga aggcaaccat ggcgaggacg
     1861 tgaaggaata ctacttctac ctcgacagca cgccgaccca ctcgtacatg aagtatctct
     1921 acaagtaccc gcaggcggcc tatccctatg cggacttggt cgagacaaac cgtcgtcgca
     1981 cccggaacga catggagtat gaactcctcg ataccggcgt cttcaaagac gaccgctact
     2041 atgacgtctt cgtggagtat gccaaggccg gtccggaaga catcctgata aaaatcacgg
     2101 ctcacaatcg cgggccggat gcagccgaac tgcatctgct gccgacgctg tggtaccgga
     2161 acgactgggc gtcgtggatc accgaatcca accgagcccc tgagaaacca aacctcaagc
     2221 agatcaagac agcggcgggc acgagcgcgg tcgcggcaac gcatcggctg ctgggtgaat
     2281 tcatcctttc ctgcgaaggc gaggtgccgc tgcttttcac ggataatacc acgaacaacg
     2341 cacggctctt tcccggtcag cccaatgccg gcccgtacgt caaggacggc atcaacaact
     2401 gcgtagtaca gggcgatcag gcggcggtga accccgaaaa gcatggcacc aaagttgcgg
     2461 cgcactaccg gatgacgatt gcctcaggcc agtcggcgac cgtgcggctg cgcctgaccg
     2521 cacgcggatc cgccgggcaa agcacaacca ctaaaagcgc tgccgtcccc ttcgggccgg
     2581 cgtttgacgc gaccttgacg gcacggttgc aggaggcgga cgagttctat cgctcggtga
     2641 cgccaccctc ggtctcgccg gacgcggcca acgtgatgcg ccaggccatc gccggcatgc
     2701 tctggagcaa gcagttcttt ttcttcgacg gtgacagttg gctggaggag caccactcca
     2761 acccgctgca tagcggatat cgtaattcgc ggaactcgga gtggttccac atgctgaacg
     2821 aggacgtcat ctccatgccc ga
//
LOCUS       DATIFQ010000378         2438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1083898, whole genome shotgun sequence.
ACCESSION   DATIFQ010000378 DATIFQ010000000
VERSION     DATIFQ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083898"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1483)
                     /locus_tag="VLE22_18395"
     CDS             complement(131..1483)
                     /locus_tag="VLE22_18395"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HSB16427.1"
                     /translation="MMKRLAGFAMAAGIALPALAQGGGQVLKIGAGTSVITPFLDGPM
                     AGYYYPRQADGVHDHLHAKALVFDNGGTRIVLVATDTVSVSAKAVEEARALLAKRLGI
                     PASHVLISATHSHTGPQFTPDYEKNLARWIADAAVSANGQAQPARLRVGIEQEPSLPH
                     YRRYRMKDGTVQTNPGFLNPGVVEPAGAIDPQVGILYADDESGKPLVTWVNYAMHLDT
                     VGGTWISADYPYFLARLLQSVKGPDMLTVFTIGAAGNINHWDVRRPGPQRGLAEAQRL
                     GEVLGAAVLKAYTHLERIERPRVAALSGTVQLPAWKSTAEEVEEARKIVGTPPTPGVD
                     FTLDRVKAERVLDVHNRGGRDYPAEIQVMCVGPVAFAGIPGELFVELGRKIRQESPFP
                     YTFIVSLANDDLGYIVTRESFEQGSYEPTSSRMAPGNGEMVAAKAVELLHELKKQTER
                     "
     gene            complement(1518..>2438)
                     /locus_tag="VLE22_18400"
     CDS             complement(1518..>2438)
                     /locus_tag="VLE22_18400"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB16428.1"
                     /translation="GIVLGLKEIGLRKFHFIEANLFHMWNYRGFVDINERLGVAMNDP
                     ELRPSTMHRANEVNWSEVPDGVVFKRIPHYAPVNEPDTWLLNIAKWKGHAMCLTQAVK
                     NEQGLVVYPYVQFCSGWRMVTGVPEFMKPDISPEVERRVLRYFENHRKLKYARYEYEE
                     NAGLSPLVQEIWAQKTCDNMSVLKTGLAMIEGIYGRDGDGFSVGDDYMANYVLFGKDK
                     FRLDLAGLWLGGHDPGNVHVYRVAKERGLSDTFNPWEVPLYEWIEGKAVPRKLTDFQR
                     IPLKSPYLPQENEPRMHLTNEPFDYDRHKV"
BASE COUNT          410 a          810 c          725 g          493 t
ORIGIN      
        1 ctgcggggat gtctttcgga ttcagtttat ctttggcagg agtctggcgc ttcatggggt
       61 cctgttccgt ttcgcggagc caacgctatc acgcggtatc cgaagttgtc aatggaatcg
      121 ggggccggat tcagcgctca gtctgcttct tcaactcgtg cagcaactcg actgccttgg
      181 ccgcaaccat ttccccgtta cccggagcca tgcgcgagct tgtcggctcg tagctgccct
      241 gttcgaacga ttcccgggtg acgatgtaac cgagatcgtc gttcgccagt gaaacgatga
      301 aggtgtacgg gaacggagac tcctggcgga tcttgcgccc gagttcgacg aacagttctc
      361 ccggaatacc ggcgaacgct accggcccga cgcacatcac ctggatctcc gccgggtagt
      421 cgcggccgcc ccggttgtga acgtcgagca cacgttcggc cttcacccgg tccagcgtga
      481 aatccacgcc cggcgtcggc ggcgtcccga cgatcttgcg cgcctcttcg acttcctcgg
      541 cggtggactt ccaggcgggc agttgcaccg taccggagag ggcggccact cgcggccgct
      601 cgatccgctc gaggtgtgtg tacgccttca gcacggccgc gccgagcacc tcgcccagcc
      661 gctgcgcctc ggcgaggcca cgttgcggcc cgggccggcg aacgtcccag tggttgatgt
      721 tgcccgccgc gccgatcgtg aagacggtca gcatgtcggg gcctttcacg ctctggagca
      781 ggcgcgccag gaagtacggg tagtcggccg aaatccaagt gccgcccacg gtgtccaggt
      841 gcatcgcgta gttcacccac gtcaccagcg gcttgccgga ttcgtcgtcc gcatagagga
      901 tgccgacctg cgggtcgatc gcgccggcgg gctccaccac gccggggttg agaaaccctg
      961 gatttgtctg caccgtgccg tctttcatgc ggtaccggcg gtagtgtggc agcgacggct
     1021 cctgttcgat gcccactcgg agccgcgccg gctgcgcctg accgtttgcc gacacggcgg
     1081 cgtccgcgat ccagcgcgcg agattcttct cgtaatcggg tgtgaactgt ggaccggtgt
     1141 gactatgcgt cgcactgatc agcacatggc tcgccggaat tccgagccgc ttcgccagca
     1201 gggcgcgcgc ctcctcaacg gctttcgctg acacactaac ggtatccgtg gcgaccagaa
     1261 cgatcctcgt cccgccgttg tcgaacacaa gggcttttgc atgcaggtga tcgtgcacgc
     1321 catcggcctg acgcggatag tagtatccgg ccattggccc gtccagaaac ggcgtgatga
     1381 cgcttgtgcc tgcgccgatc ttgagaacct gcccaccgcc ctgtgcgagc gcaggcaacg
     1441 caatgccagc ggccatagcg aaccccgcca gacgcttcat catccggact cccttcccta
     1501 ttccagccgc gtgctcccta gaccttgtgc cggtcatagt cgaacggctc gttagtcagg
     1561 tgcatccggg gctcgttctc ttggggcaga tacggcgact tgagcggaat ccgctggaag
     1621 tcagtcagct ttcgcggaac cgctttccct tcgatccact cgtagagggg cacttcccat
     1681 ggattgaacg tgtcggacaa cccgcgctcc ttggcaacgc ggtacacgtg cacgttgccc
     1741 gggtcatgcc cgccgagcca gaggcctgcc aggtccagcc ggaacttgtc cttcccgaaa
     1801 agcacatagt tcgccatata gtcgtcgccg acgctaaagc cgtcgccgtc gcggccgtaa
     1861 atgccttcga tcatggcaag ccccgtcttg agcacgctca tgttgtcgca cgttttctgc
     1921 gcccagatct cctgcaccag aggcgaaagc ccggcgttct cctcatactc gtagcgggcg
     1981 tacttcagct tcctgtggtt ctcaaagtag cggagcacgc gtcgttccac ctccggagag
     2041 atatcaggct tcatgaactc cggcacgccg gtcaccatcc gccaccccga gcagaactgc
     2101 acgtacgggt agaccacgag tccctgttcg ttcttcaccg cctgcgtcag gcacatcgcg
     2161 tgccccttcc acttggcgat gttcagcagc caggtgtccg gttcgttcac cggcgcgtag
     2221 tgaggaatgc gcttgaatac gacgccgtcc ggcacttcgc tccagttcac ctcgttggcg
     2281 cggtgcatcg tgctcggccg caactcggga tcgttcatgg ccacaccgag ccgttcgttg
     2341 atgtccacaa acccgcggta gttccacatg tggaacaggt tcgcctcgat gaaatggaac
     2401 ttccggagcc cgatctcctt caggccgaga acgatgcc
//
LOCUS       DATIFQ010000379         3753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2043155, whole genome shotgun sequence.
ACCESSION   DATIFQ010000379 DATIFQ010000000
VERSION     DATIFQ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043155"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            928..2241
                     /locus_tag="VLE22_18405"
     CDS             928..2241
                     /locus_tag="VLE22_18405"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HSB16429.1"
                     /translation="MKPTGASPGKPVVTGDTATRAGAAAVLSGTSARRGLARLMPFLG
                     PAFIASIAYVDPGNFATNIQGGAQFGYTLLWVVVASNLMAMLIQTLSAKLGIATGHNL
                     AELCREHLPRPVVWVMWLLMELVAMATDLAEFLGAAVGFNLLFGLPLWVAGILTAVAT
                     FFILGLENKGFRPVEAVITALLGVIAVCYVAETFLDRPDWALVAFHAVVPRFAGAESV
                     LLATGILGATVMPHVIFLHSALTQGRIRVEGTEHKRRLFRFQLVDVSIAMGMAGFVNA
                     AMLMMAAATFHRAGMHNVGTLEEAYRTLDPLLGPAAPTLFAVSLLAAGLSSSAVGTMA
                     GQVIMQGFLHRQIPVWVRRLVTMAPSIVVIMIGVDPTRTLVISQVVLSFGLPFAVIPL
                     VMFSRRKDLMGSLVNHGATTGLAGLVAALILLLNGYLLFKTFFGG"
     gene            2244..3155
                     /locus_tag="VLE22_18410"
     CDS             2244..3155
                     /locus_tag="VLE22_18410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSB16430.1"
                     /translation="MFRKILVPLDGSRLAEAALPVARWFADRLGSEVTLLHVMEQKPP
                     PRVHGEHHLTRPEEAAAYLEAIAASAFGPGQTVAAHVHTTSTAQVAHSIAEHRAELAA
                     DLIVMCGHGDSGLRDLLFGSVAQRASALGTIPVLFVRARAVHAPTPFHCGKLLVPTDG
                     EPHHEQGLAAAREIARPAGALVLLLSVVPTWSQLRGRSLTSARMLPGTTQAALEMTAE
                     SLRAHLGELASELRQEGFEATAEVQRGDPAARIVATAAETRADLIVMATHGKTGGSAF
                     WEGSVTPKVLKDTFVPVLLVPVAAGRA"
     gene            complement(3368..3568)
                     /locus_tag="VLE22_18415"
     CDS             complement(3368..3568)
                     /locus_tag="VLE22_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16431.1"
                     /translation="MRGQGRQKVRLSTPGEALIFFMIRLIERLRAYGSAPAVDLMEYG
                     RGLESFGGTKHRSGVSLDHKDH"
BASE COUNT          640 a         1192 c         1220 g          701 t
ORIGIN      
        1 gcgatgccag agcgatgagc tgcccgaaac gagaagggat cccggagacg gtttcactca
       61 aggttggtgc cagtcgccgg gtcgtagtgg cttccgctga tatccaagag acaacttcgg
      121 aactcatggc acacccgaaa aggcgacggg ccacgatgac aagcaacccg tttgacacct
      181 taggaagttc atccctaccc agggacagct tcaagatata gtcttgctag aacataagcc
      241 aatagtacct ttaactcaat tttggagtgc ggaagcgcgc agcgttcccc gccgccgggg
      301 ggagtcgtcc ctaacgtttt gcagcgagca ctccgggagc ctggtaatga gtcgggtgaa
      361 gaaggcggtg cgcagtgttg gatccgtgac gctggagagg tccacttcct caacggggat
      421 gtcgatttgc tttttcgcgt gtaggcgcgt cgctggttcg ccgttaacga acaatccacg
      481 tcgcgactgc tgaaacaatg ccgggaccgg acttccgact ggttcagact atgcgaacca
      541 aagcagaatt aaagcacatc tcgggtgccc gaacaatgtc tttcctggat gttggctgga
      601 tgtcagagcc ggacggtttc atgtcgctgg atgattcctg acgataacag cccttccgtc
      661 caacaaccgg atcattctcg cgaaaggcct tcttgaatac tggcagcagg tattaaaacg
      721 cagtcggcag ccgcggtcca tcgcaggctc ggggcgcggc ctggcctcgc gtcggctggg
      781 agaactccat aaagctggcc cgcgtcgcct ccctggcggc ccgcgcctgc ggcgggcggc
      841 tcggagcgcg gcggggcccg agaactctga agtgcgaccg gcttggatgc agtatccgcg
      901 aggcatgcac ctctgtatac tgcggtcatg aagccaaccg gcgcttcgcc gggcaagccc
      961 gtggtgacgg gcgataccgc cacgcgagcg ggggcggcgg cggtactctc gggcacgagt
     1021 gctcgacggg gactggctcg tctgatgccg ttcctgggac cggcattcat cgcaagcatt
     1081 gcctacgtcg accccggcaa cttcgctacc aacatccagg gcggggccca gttcggctac
     1141 acgctgctgt gggtggtggt ggcgagcaat ctgatggcga tgctgatcca gacgctatcg
     1201 gcgaaactgg gcatcgctac cgggcacaac ctagcggagc tgtgccgcga gcacctccct
     1261 cggccggtgg tgtgggttat gtggctgctg atggaactgg tggcgatggc cacggacctg
     1321 gccgagttcc tgggcgcagc cgtcgggttc aacctgctgt ttggcctgcc gctctgggtg
     1381 gccgggatct tgacggcggt ggcgacgttc ttcatcctcg gtctggagaa caagggcttc
     1441 cggccagtgg aggcggtgat caccgctcta ctgggggtga tcgccgtttg ctacgtggcg
     1501 gagacgttcc tcgaccggcc tgactgggcg ctggtggcgt ttcacgcggt ggtaccccgc
     1561 ttcgccggcg ccgagagcgt cctgctagcc accggcatac tcggcgccac ggtgatgccc
     1621 catgtcattt tcctgcactc ggcgcttacc cagggccgca tccgggtcga gggaacggag
     1681 cacaagcgcc gcctgttccg cttccagttg gtcgacgtgt cgatcgcaat gggaatggcg
     1741 ggcttcgtca acgcggcgat gctgatgatg gcggcggcga cgttccatcg cgccggcatg
     1801 cacaatgtgg gcacattgga agaggcctac cgtactctcg atccgctgct cggccccgcc
     1861 gcccctacac tgttcgcggt ctcgctgctc gctgcgggcc tgtcgtcctc cgcggtgggc
     1921 acgatggccg ggcaggttat catgcagggg ttcctccacc gccagatccc ggtgtgggtg
     1981 cgccggctgg tcacgatggc gccgtccatt gtcgtgatta tgatcggcgt ggaccccact
     2041 cggacgctcg tgatcagcca ggttgtgctg agcttcggcc tacccttcgc ggtgatcccg
     2101 ttggtgatgt tcagccgccg gaaggatctc atgggcagcc tggtaaacca cggggcgacg
     2161 actgggctgg ccgggctggt ggccgccctc atcctgttgc tgaacgggta cctgctgttc
     2221 aagacgtttt tcggaggata agcatgttcc ggaagatcct ggtccccctc gacgggtcgc
     2281 gcctggcgga ggcggcattg ccggtggcga ggtggttcgc cgaccgcctg gggtcggagg
     2341 tcaccctgct tcacgtgatg gagcagaagc ccccgccccg ggtgcacggt gagcaccact
     2401 tgacccggcc tgaggaggcg gccgcctacc tcgaggcgat agcggcaagc gccttcgggc
     2461 cggggcagac ggtggcggcc cacgtccaca ctacctccac tgcccaggtg gcccactcca
     2521 tcgcggagca ccgcgcggag ctggcagcgg acctcatcgt catgtgcggc cacggcgaca
     2581 gcggcctgcg cgacctgctg ttcggcagcg tcgcccaacg ggccagcgcc ctcggcacga
     2641 ttccggtact cttcgtccgg gcgcgggcag tccatgcccc cacgccgttc cactgcggca
     2701 agctcctggt gccaacagac ggcgagccgc atcacgagca gggcctcgct gcggcccggg
     2761 agatcgcccg ccccgccggc gctctggtcc tgctgctgtc ggtcgtcccg acctggtcgc
     2821 agctccgggg ccgctccctc acctcagccc ggatgctgcc agggaccacc caggccgcgc
     2881 tggagatgac cgcagagagc cttcgggccc acctcggaga gctggcgtcg gagcttcgtc
     2941 aggagggttt cgaggcgacc gccgaggtcc agcgcggcga ccccgcggcg cgcatcgtcg
     3001 cgacagccgc cgagactcgg gccgacctta tcgtgatggc gacccacggc aagaccggag
     3061 gcagcgcctt ctgggaaggc agtgtcaccc caaaggtgct gaaagacacg ttcgtcccgg
     3121 ttctgctggt gccggtggcc gccggccgcg cttgactgtg accggaaggt gacaaggagg
     3181 tgtcggtcat caaaatccca tttgcaaaca gagagacgtt gcttttctct gccatgtgtg
     3241 gcggcggtcg tccagatagc ttgcgacaac agcccttatc accagagacc gggctcggtt
     3301 ccgaacggtg ttgcggaaca ataggtcctg actggtcggc aagggtttgc tgacgatccg
     3361 tgggccgtca gtggtctttg tggtcgaggg acactcctga cctgtgcttt gtccccccaa
     3421 aactctccag ccccctcccg tactccatca agtccaccgc cggcgcgctc ccatacgccc
     3481 gcagccgctc gatcagccgg atcataaaga agatcagcgc ttccccggga gtgctcagcc
     3541 gcaccttctg ccgtccttga ccacgcattg aagatcatcg tcatgaactg tatggagtgt
     3601 caaaaagggc aaatgcgccg gaagctaact cgcccggcgc tcgtggacct caatgagcaa
     3661 accgcgaccg taggaagcca aacagggatt gcttggcctt catacgtccg ctgcgccggc
     3721 gaactccgtc aagctgaaac agtacccccg tgt
//
LOCUS       DATIFQ010000380        11372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1657002, whole genome shotgun sequence.
ACCESSION   DATIFQ010000380 DATIFQ010000000
VERSION     DATIFQ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11372
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657002"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VLE22_18420"
     CDS             <1..763
                     /locus_tag="VLE22_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16432.1"
                     /translation="ALWMTHNLVWAVAALAVGRLAVLLAYDRHAGAAGENLSRSGWRA
                     ELAIFRTALPLGVVLMLVSLNANLPRYAVEHHLGTRDLGVFAAVVSFITVGSTIVNAL
                     GQTSTPRLARYFSECNLPRFRQLIFRLAGLVLLLGASGVLVAMFLGKFVLRLIYRPEY
                     EAHSGLLVAVMGAATLSYLAIALGYAVTGARVFRAQMPLFCAVAACCGVASWLLVPKL
                     GLYGAVLALALASSLQIAGQLLILAWAIRRMETAK"
     gene            760..1959
                     /locus_tag="VLE22_18425"
     CDS             760..1959
                     /locus_tag="VLE22_18425"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSB16433.1"
                     /translation="MRLRLPPSALDRAAWALLCLFVFSVPWEKSVWVHGIGTISRLLG
                     IVAFAAGAVVAFRRRAIRPPNIALVLAGIFVLWSALTYGWSLDRPATRVRVVTLVELF
                     AMLWLVWDSCRRPLRQRRLIEAYVLGAVTGSCVAFVRYIHDLQTYYRRYAAPGFDPND
                     FGLVLALSIPLSLYLALSGRGRMRWCWRAATLIVISAVLLTASRTALIATFIAFSFAL
                     WTWRKADFSQRVSSVLLAALLALGLLRFAPAPSRQRLATIPSEISRGTFHGRAEIWKS
                     GVRAWKSHPVLGVGSGAYPEAVRPWLGRPDVPGIQYVAHNTFLSVLVECGVIGFGLYA
                     LLLGVLFLFVWMMPSPERALWAVMLAVWAAGVSTLTWEHYKPTWLMFALIMTEWARSW
                     WPTGKGE"
     gene            1956..3068
                     /locus_tag="VLE22_18430"
     CDS             1956..3068
                     /locus_tag="VLE22_18430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB16434.1"
                     /translation="MKIRVVEVLASLRRAGAERMAVSLACGLDPDRFETRVVSLFDAF
                     PEGFEPVLGERRVPVDHLGKRRGMDLRMYPRLMRVFRRFRPDIIHTHSYVLRYAFPAS
                     VATRAGSMVHSVHNVALRETEWLGRLIHRVAFRRGVVPVAVAAEVARSFQKVYGFAPA
                     ATIPNGIDLDRFLQPDAGPEWRRANGFSTDDHLVVSVARLEPQKNPLGLVDAFARALP
                     DDARWHLLLVGDGSLREEVAGHVARLRLGHRVHLLGTRSDVAALLSAADAFVLASHWE
                     GNPMAVMEAMAAGLPVVATAAGGVPEVVEHGVTGLLAPPGDTEALASALAELARDPQR
                     RDEFRQAALRGVPRFSLNAMIASYSELFVSLARGRS"
     gene            3065..4213
                     /locus_tag="VLE22_18435"
     CDS             3065..4213
                     /locus_tag="VLE22_18435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB16435.1"
                     /translation="MRTPVMLLATNLARGGAEMQVALLALALRRHGWNVSVASLLEPS
                     AFVEELAACGIPVSSLRMRPGKVDPLAVTRFFSMLRRSRPSILHAHMFHANLMARSIR
                     LVCPVPVLISTIHSIAESSRRSAGVRRRDWLYRATDCLSDATVCVSAAGAKRYAAIRA
                     VSRDRLRVIPNGVDTDLFRPDPGSRARMREVLGVGDEFTWLAVGRLMWKKDYVTMLEA
                     MARQREGVLLIAGAGPLEEKLCALAGELGVRVHFLGLREDIVDLMNASDGLVLSSVVE
                     GLPMVLIEAAATGLPCVATDVGGVREAVLDGQTGFVVRIQDPIALAAAMRRLAELAPE
                     ARVRMAESARAHALAQFDMRTVVAQWEQLYLDRLEAARLCGMEPPVDD"
     gene            4218..5006
                     /locus_tag="VLE22_18440"
     CDS             4218..5006
                     /locus_tag="VLE22_18440"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB16436.1"
                     /translation="MSVLPEMDVTNRFVFDFAMRFARDNPGARILDFGCGGGRLVKAG
                     LDSGLAISGADVFYSGSRARGEAERSGLLGTAIQEIHDGQIGFADSSFDLVVNNQVME
                     HVANLDAVLREIHRVLRSGGEVLSLFPSRDVFREGHIGIPFSHRLPKDSKLRFYYTWA
                     LRGLGCGTWKEQAPTCRQWAVDKLWWIDTYTFYRSRGEISRTFGRYFTSEARELDYIR
                     YRLLDRPGRRWLAGLLDFPAVPILARALFRKLAFLVILSRKEAR"
     gene            5003..6127
                     /locus_tag="VLE22_18445"
     CDS             5003..6127
                     /locus_tag="VLE22_18445"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB16437.1"
                     /translation="MKIAYVITRADAVGGASIHVRDLAQAMLERGHQPVVLVGGLGPV
                     TDQLASAGVPFRSLENLQRSIHPVRDFRARAEMIAVLRELRPDLVSTHTAKAGWIGRA
                     VGAQLGLPALYTPHGWSIGNRISPTLGAVFAVAERTAAKWASAIVCVCEYERRLAIEK
                     GIAAPEKLVVVHNGMRDIPAGLHAEPGLSPVRICSVARFESPKDHATLLRALAALRKK
                     DWQIDLIGDGPLEPEMRKLAAALNIADRVRFLGYLPDPAPVLARAQLFVLSSRSEAFP
                     RSVLEGMRAGLPVVASNVGGVGEAVTDGVNGVLVRARSVDALSAALAGMIENVSRRQL
                     LGRAARHVFEERFRLERMVEQTAAVYATVLSRTAKIQRID"
     gene            6129..6821
                     /locus_tag="VLE22_18450"
     CDS             6129..6821
                     /locus_tag="VLE22_18450"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HSB16438.1"
                     /translation="MTSPWNKSGVPVEESFAAQMCGGLFHASAQGLKRIFDIVSALVL
                     IVLALPFAVVIALAIVLETPGPVFFGHTRIGRGSRRFRLWKFRSMVVNSEDVLRAYLE
                     KRPDLATEWNATHKLKDDPRVTRVGRFLRRRSLDELPQMWNVLRGDMSLIGPRPIVED
                     EIPKYGTAFDLYRRVRPGLTGLWQVSGRSDTSYRKRIELDSRYIRSWSIWLDLRVIWK
                     TVGVILRAHGAY"
     gene            complement(7019..8317)
                     /locus_tag="VLE22_18455"
     CDS             complement(7019..8317)
                     /locus_tag="VLE22_18455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB16439.1"
                     /translation="MTLRLKTFLYLVFIHLVLASTAVFFLRERRIWLLAVEAFLVLSA
                     VLGIRIFNGIFGPLQLIRTGIQFLSESDLNSRFREVGQKELDDLIQVYNQMIDHLRDE
                     RIRVQEQHHFLSQIVEASPSGIIIFDFDDRISSVNPSAEEMFKVRELAGKKLQELDGA
                     FAEALSSVQTGTSRIIPLEGIRRVICTKSRFLDRGFQRNFLMLEELTEELRRSERAAY
                     EKLIRMMSHEINNSVGASNSLLQSCLNYKDQLQPEDREDFEMALSVAISRAHHLNAFV
                     RSYADIIRLPQPELRPCDVKTLLEDIAVLMRAECHEKGITWKWQIEDAVGLVPIDKNQ
                     MEQVFVNILRNSIEAIDENGVITIRMGKRSGRTFVAVEDTGRGITPEIRANLFTPFFS
                     TKENGRGIGLTMIQEILTRHGFNFSLDGQPGRPTEFCIYF"
     gene            complement(8314..9693)
                     /locus_tag="VLE22_18460"
     CDS             complement(8314..9693)
                     /locus_tag="VLE22_18460"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB16440.1"
                     /translation="MNKAILIVDDDASVTTSLALLLKQAGYRSQSARSPNEAVRRMEL
                     EEFALVIQDMNFSRKTAGEEGLALLQALKERRPHMPVILITAWGSISLAVQGMKAGAT
                     DFITKPWTNQQLLQSVETALGLAAANARAGQRALSREELDSLYDFRMVVGRDPKLLKV
                     LEVIGRIASTEASVLITGESGTGKEVIAEAIHRNSKRKNAAFVKVNLGGISSTLFESE
                     MFGHVKGAFTDARQDRKGRFEIANGGTIFLDEIGELDPNSQVKLLRVLQDRTYEVLGS
                     SATRAVDVRVISATNQNLPELVKSGGFREDLLYRLNLIAIHLPALRERPADVPLLVRH
                     FLESIGLVYRRQDLGISDSALKWLQGMSWPGNVRQLKQLIERTVLVTGNDLLDVDHLA
                     VALGMESREVQKDGLPAVGAMTLDEMEESMILKCMQHYGGNVSKVAEALGLSRPALYR
                     RFEKYGITP"
     gene            complement(9736..10947)
                     /locus_tag="VLE22_18465"
     CDS             complement(9736..10947)
                     /locus_tag="VLE22_18465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB16441.1"
                     /translation="MIKHMFKLVWNRKRTNILIITEIFFSFLVLFGVTAFGIYYTDNY
                     LRPLGFSYENVWNVSVSGIFDSRHAERAQAMNDFKQMQLALRDLDEIEGVAGIGMTPF
                     QIGASNGPICYRGRSAEARYNDGTDELKDVLELKVVQGRWFDSSDDSLNYEPVVINVR
                     LRDELFGHENPLGKEFDLPKDPEERAKTMPQRVVGVITDFRQHGEFHELYNYCFHRAS
                     AKKLSGLAMIALLVKLRPGTTAAFEEKLMNRLRSVMKDRTIQIEPLDRARKSHMRLVL
                     APMAAASLVAVFLIIMVGLGMVGVLWQNVSRRTKEIGLRRALGGTAGDVHRQVLGELL
                     VIATAGLLLGMAVVVQLPLLEIVDWMSPGVFTVGLIVSLLVMYGLTIAAGLYPSWLAT
                     KVQPAGALHYE"
     gene            complement(10944..>11372)
                     /locus_tag="VLE22_18470"
     CDS             complement(10944..>11372)
                     /locus_tag="VLE22_18470"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSB16442.1"
                     /translation="SDYAREKTSRLVLWLLFYALLFMLLPTVNLININVSRILERASE
                     IGVRKTFGASSLTLVGQFVMENVLLTLIGGLIGFVLSAVVLRGITAAGILAYAQFQVN
                     YRVFLIGMLMTLFFGLFSGVYPAWRMSRLHPVEALRGASR"
BASE COUNT         2041 a         3477 c         3458 g         2396 t
ORIGIN      
        1 cgcactctgg atgacgcaca atctggtgtg ggctgtggcc gccctcgccg tcggacgcct
       61 ggcagtgctt ctggcctacg atcgtcatgc gggcgcagcc ggagagaatc tgtcccgctc
      121 cggttggcgg gcggagctgg cgatcttccg gaccgcgctg cccctcgggg ttgtactcat
      181 gctggtgtcg ttaaacgcca atctgccacg ctatgccgtc gagcatcacc tcggtactcg
      241 cgacctcggg gtgttcgccg ccgtggtgtc attcatcacc gtgggcagca cgatcgtgaa
      301 tgcactaggc cagacgtcca cgccccgcct cgcacgctat ttcagcgagt gcaacctgcc
      361 gcgattccgt caactgatct tcaggctggc cgggctggtg ttgcttctgg gggcctcggg
      421 agtgctcgtc gccatgtttc tcgggaagtt cgttctccgt ctcatctacc ggccggagta
      481 tgaagcacac agcggactcc tcgttgcggt gatgggagcg gccacgctga gctatctggc
      541 aatcgctctt ggctacgccg tcaccggcgc ccgtgtcttc cgagcgcaga tgccgctatt
      601 ctgcgccgtc gcggcatgct gtggagtcgc aagttggctg ctggttccga aactcggctt
      661 atacggtgcg gtcctggctc ttgccctcgc ctcctcgctc cagatagccg gtcaactcct
      721 gatcctcgcg tgggcgattc gccgaatgga gacagcgaag tgaggctccg gcttccaccg
      781 agcgcactcg atcgtgccgc ctgggcgctc ctgtgtctgt tcgtcttttc ggttccttgg
      841 gagaaaagtg tctgggtgca cggaatcggc acgatttcgc gactgctcgg catcgtcgcc
      901 ttcgcggccg gcgccgtcgt tgctttccgg cgccgggcga ttcgcccgcc caacattgcc
      961 ctggtgctgg cgggcatctt cgtgttatgg tccgcactga cctacgggtg gagcctcgac
     1021 cggccagcga ccagggtgcg ggtcgtcact ctcgtggaac tgttcgcgat gttgtggctc
     1081 gtctgggact cctgccgcag gccgctacgt caacgccggc tcatcgaagc ctatgtgctc
     1141 ggtgcggtca ccggctcctg cgttgccttc gtccgttaca ttcacgacct gcaaacgtac
     1201 tatcgacgct acgcggcccc ggggttcgat ccgaacgatt tcgggttggt actcgcgctg
     1261 tccataccgc tgtcgctcta tctggccctg agcgggcgtg gaaggatgcg ctggtgctgg
     1321 cgcgccgcaa ccctgatcgt gatttccgcg gtgctgctca cggcgtctcg aaccgctctg
     1381 atcgcgacgt ttatcgcctt cagctttgcc ctttggacgt ggcgcaaagc cgacttctct
     1441 cagcgagttt cttctgtgtt gcttgcagca ctactggccc tgggtctgct tcggtttgct
     1501 ccggctccgt cccggcagcg gctcgcgacg attccgtcgg agatctccag gggcacattt
     1561 cacggccgcg cggagatctg gaagagcgga gtcagggcct ggaagagtca cccggtcctc
     1621 ggcgtcggct cgggtgcata tccggaggcc gtgcgcccgt ggctcggccg gccggacgtg
     1681 cccggcatcc aatacgtcgc tcacaatacg tttctctctg tgctggtcga gtgcggtgtg
     1741 attggcttcg ggttgtacgc gcttctcctc ggcgtcttgt ttctcttcgt ctggatgatg
     1801 ccttcccccg agcgtgccct ctgggccgtg atgcttgcgg tctgggcagc cggcgtttcg
     1861 actctcacct gggagcacta caagccaact tggctgatgt ttgcccttat catgacggag
     1921 tgggcgcggt cctggtggcc cacaggcaag ggcgagtgaa gattcgggtc gtcgaagtgc
     1981 tggcttcgct acggcgcgcg ggtgccgagc ggatggctgt gtcgctggcg tgcggcctgg
     2041 acccggatcg gtttgaaacc cgagtggtgt ccctcttcga cgcttttccg gagggatttg
     2101 agccagtgct cggcgagcgc cgcgtccccg tggatcacct gggaaaacga aggggcatgg
     2161 acctccgcat gtatccacgg ttgatgcgcg tgttccgccg gttcagaccc gacattatcc
     2221 acacgcactc ctacgtgctt cgctatgcgt ttcccgcgag tgtggccacc cgcgcgggtt
     2281 ccatggtcca cagcgtgcac aacgtcgcct tgcgggaaac ggagtggctc ggccgcttga
     2341 ttcatcgcgt cgcatttcga cgaggggtgg tccctgtcgc ggtggccgcg gaagtcgccc
     2401 ggtcgtttca aaaggtttac ggattcgccc ctgcggccac aatcccgaat ggcatcgact
     2461 tggatcgctt cctccagccg gatgccgggc ccgagtggag acgcgcaaac ggcttttcca
     2521 ccgatgacca cctcgttgtc tcggtggcgc gcctggaacc ccagaagaac cccctcggac
     2581 tggtcgacgc gttcgcccgc gcgttgcccg atgacgcccg atggcatctg ctgctcgtcg
     2641 gcgacggaag ccttcgagag gaggtggccg gacacgtcgc gcgcttgcgt ctcgggcatc
     2701 gcgtgcatct gctcggcacc cgttccgacg tggcggcttt gttgtccgcc gccgacgcgt
     2761 tcgtcttggc ctcgcactgg gagggaaacc cgatggcggt gatggaagct atggctgccg
     2821 ggctgcccgt ggtggcgacc gccgccggtg gcgtgccgga agtcgtcgag cacggagtga
     2881 ccggactcct ggcgccgccg ggtgacaccg aggcgctggc ctccgctctg gctgagcttg
     2941 cccgggaccc gcagcgccgt gatgaattcc ggcaggccgc cctccgcggc gttccgcgat
     3001 tcagtctcaa cgcgatgatc gcgtcttact ccgaactctt cgtgagcctt gccagagggc
     3061 ggtcatgagg acgcccgtca tgctgcttgc gaccaatctc gcccggggcg gcgcggaaat
     3121 gcaggtcgcg ctgctcgccc tggccttgcg ccgccacgga tggaacgtaa gcgtcgcctc
     3181 gctgctcgaa ccgagtgcct tcgtcgaaga gctagccgcg tgcgggattc cggtgtcctc
     3241 tctcaggatg cggccaggca aggtggaccc gctcgcggta acccgcttct tctctatgtt
     3301 gcggagatcc agaccgtcca tcctgcacgc gcacatgttc catgccaacc tgatggcgcg
     3361 gtccatccgc cttgtctgtc ccgttcccgt ccttatatcg accatccaca gcatcgcgga
     3421 gagcagccgc cgctcggccg gcgttcgccg ccgggactgg ctttaccgtg ccacggactg
     3481 tttgtcggac gcgaccgtct gtgtcagtgc ggccggggcg aagcgctatg ccgccattcg
     3541 ggctgtgtcg cgagacaggc ttcgcgtgat ccccaacggc gtcgatacgg accttttccg
     3601 gccagatccc ggttcacggg cgcggatgcg cgaggtgctg ggtgtcggcg acgaattcac
     3661 ctggctggcg gtcgggcggc tgatgtggaa gaaggactac gttacgatgc tggaagcgat
     3721 ggcgcggcaa cgcgaggggg tgctgctcat cgccggcgcc ggcccgctgg aggaaaagct
     3781 gtgtgcgctc gccggcgagc ttggagtcag ggtccatttc ctcggcttgc gcgaggacat
     3841 cgtggacctg atgaacgcct ccgacggtct ggtgttgtca tcggtcgtgg aaggtctgcc
     3901 gatggtgctg atcgaggcgg ccgccaccgg cctcccctgc gtcgcgaccg acgtcggcgg
     3961 cgtacgcgaa gcggtcctcg atgggcagac cgggttcgtg gtccggatcc aggatcccat
     4021 cgccttggcc gcggcaatga ggcgcctcgc ggagttggcc ccggaagctc gagtgcgcat
     4081 ggcggaatct gcccgagcac acgccctcgc gcagttcgac atgcgcaccg tcgtggcgca
     4141 gtgggagcaa ctctatctcg atcggctgga ggccgcgcgg ctctgcggaa tggaaccacc
     4201 cgtggacgac tgacgacatg agtgttcttc cggaaatgga cgtcaccaac cggttcgttt
     4261 tcgacttcgc catgaggttc gcccgcgaca accccggcgc tcgcatcctc gacttcggtt
     4321 gcggcggcgg tcgcctggtg aaggcgggtc tcgactcggg ccttgcgatt tcgggagcgg
     4381 acgtcttcta ctcgggatcg agggcccgcg gagaggctga acgctcaggc ctgctcggca
     4441 cggccatcca ggagatccat gacgggcaaa tcggcttcgc ggactcatcc ttcgatctgg
     4501 tggtaaataa ccaggtgatg gagcacgtcg cgaacctcga cgccgtgctc cgtgagattc
     4561 accgggtcct caggtccggc ggcgaagtgc tgagcctctt cccgtcgcgc gacgtcttcc
     4621 gggaaggcca tatcggcatt cccttctcgc accgcttgcc caaagattca aagcttcgtt
     4681 tctactacac ctgggcgttg cgtggactcg ggtgcggaac ctggaaggag caggctccca
     4741 cctgccggca gtgggcggtg gacaagctgt ggtggatcga cacctacacc ttctatcgca
     4801 gccgtggtga gatctcccgt acgttcggcc gctactttac gagcgaggca cgggaactcg
     4861 actacatccg gtatcgtctg ctggatcgtc ccggacggcg atggctcgcg gggttgctgg
     4921 acttcccggc agttcccatc cttgcccgcg cactctttcg gaagcttgcg ttcctggtga
     4981 tcctctctcg aaaggaagca cggtgaagat cgcctacgtc ataacgcgcg cggatgccgt
     5041 cggaggtgcg agcattcacg tccgggacct ggcgcaggcg atgctggagc gtgggcatca
     5101 gccggttgtg cttgtcggcg gcctcggccc ggtaacggac caactggcct ccgccggcgt
     5161 cccgtttcgc tcactcgaaa accttcagcg gtccatccat ccggtccgcg acttccgcgc
     5221 gcgggccgag atgatcgccg tgctgcgcga attgcgcccg gacctggttt cgacgcacac
     5281 cgccaaggcc ggctggatcg ggcgggcggt tggcgcgcag ctcggtcttc cagcgctcta
     5341 cacgccccac ggttggtcga tcgggaatcg gatttccccc acgctcggtg cagtcttcgc
     5401 tgtggcagag cgcacggcgg cgaaatgggc atccgcgatc gtgtgcgtct gcgagtacga
     5461 gaggcggcta gcgatcgaga aaggaatcgc cgccccggag aaactcgtcg tggtacacaa
     5521 cgggatgcgc gacattcccg ccgggttgca cgccgaaccg gggctgtcgc ccgttcgcat
     5581 ctgctccgtg gcgcgtttcg aatcgccgaa ggaccatgcc accctgctta gggccctcgc
     5641 ggctcttcgg aagaaggact ggcagatcga cttgattggg gatggaccgc tcgaaccgga
     5701 gatgcgaaag ctggccgctg ctctgaacat cgcagatcga gtccgctttc tcggttacct
     5761 tccagacccg gcgcctgtgc tcgccagagc gcaactgttc gtcctctcct cgcggtcgga
     5821 agcgtttcca cggagcgtgc tggaagggat gcgagccggg ctcccggtag tcgcctccaa
     5881 cgtcggcggc gtcggggagg ccgtgacgga cggcgttaac ggcgtgctgg tccgtgcccg
     5941 gagtgttgat gcgctctctg ccgcgctcgc cggcatgatc gaaaatgtgt cccggcggca
     6001 actcctcggc agggcggcgc gtcatgtatt tgaggagcgg ttccggctgg agcgcatggt
     6061 cgaacagact gccgctgtat atgctacggt gctaagcaga accgcgaaaa tccagcgaat
     6121 cgattaacgt gacctcccct tggaacaaat ccggcgtccc ggtcgaagag agcttcgccg
     6181 cgcaaatgtg cggaggtctg ttccacgcca gcgcgcaagg cctcaagcgg atcttcgaca
     6241 tagtgtccgc tttggtcctg atcgtactcg cgttgccgtt cgcggtcgtg atcgctctgg
     6301 caatcgttct ggagacgcct ggccccgtgt tctttggcca cacccggatc ggaaggggta
     6361 gccggcgatt tcgtctgtgg aagtttcgct cgatggttgt aaacagcgaa gacgtcctcc
     6421 gcgcttacct cgaaaagagg ccggacctcg caacggaatg gaacgcgacg cacaagctga
     6481 aagatgatcc ccgcgtcaca cgggtcggcc gtttcctgcg ccgaaggagc ctcgatgaat
     6541 tgccccagat gtggaacgtg ttgcggggcg acatgagcct gatcggcccg cggccgatcg
     6601 tcgaggatga aatcccgaag tacggtacgg cgttcgatct ttaccgaagg gtacgccccg
     6661 gactcaccgg attatggcag gtttcgggac gcagtgatac tagctaccgc aagcgcatcg
     6721 aattggattc ccggtacatc cgttcctggt caatctggct ggatctgcgc gtaatctgga
     6781 agacagtcgg cgtcatcctg cgcgcgcacg gtgcttattg aagtgacctg ataaacgcca
     6841 tccgtcgtag tcggcggtcc gaagccggtt cgttcggtcc gcagcagaag gggaacagag
     6901 gcacgctcca gtgaatgagg tgtgtgagtc ccacccctgt tcgccgatac gcagctgacg
     6961 tgcagggaga cgatgatgca gcttgggtct gtcacaggta gcccgtcatg agcggctttc
     7021 agaaatagat gcaaaactct gttggccggc cgggttgacc gtcgagcgag aaattgaagc
     7081 catgacgtgt caggatttcc tggatcatcg tcagaccgat gccgcggcca ttttccttcg
     7141 tgctgaaaaa cggagtgaag agattggctc gtatttcagg cgtgatcccg cggcccgtgt
     7201 cctctaccgc aacgaaagtc cggcctgatc ttttgcccat gcggattgtg atgacgccgt
     7261 tctcgtcgat cgcctcaatc gagttgcgca gaatgttaac aaagacctgt tccatctgat
     7321 tcttgtcgat cgggaccagt ccgacggcat cctcgatctg ccacttccag gtgatccctt
     7381 tctcgtggca ctctgctcgc ataagaacgg caatgtcctc aagaagcgtc ttcacgtcgc
     7441 aagggcgcaa ttccggctgt ggcagccgga tgatgtccgc gtaactgcgt acgaaggcgt
     7501 tcagatggtg agctcgtgag atggccacac tcaaagccat ctcaaaatct tcacggtctt
     7561 caggctgcag ttggtccttg tagttgaggc aggattgcag gagcgaattg ctcgcgccaa
     7621 cggagttatt gatctcatgc gacatcatcc ggatgagctt ctcgtacgcg gctcgttcgg
     7681 accgccgcag ttcctcggtg agttcctcca gcatcagaaa gttgcgctgg aacccgcgat
     7741 ccaaaaaccg cgacttcgtg catatgaccc gtcggattcc ttcgagagga atgatccgcg
     7801 acgtccctgt ttggacagaa ctcaatgcct cggcaaacgc tccgtccagc tcctgcaact
     7861 tctttcccgc gagttcccgc accttgaaca tctcttccgc gctggggttc accgacgaga
     7921 tacgatcgtc gaaatcgaag atgataattc cggacggcga tgcctccacg atctggctca
     7981 ggaaatggtg ctgttcctgc acgcggatcc gctcgtcgcg aaggtgatcg atcatctggt
     8041 tgtagacctg gatcaggtca tcgagctcct tctgtcccac ttcccggaat cgcgaattca
     8101 agtcgctctc gctgaggaac tggattccgg tccggatcaa ttgaagtggg ccgaaaatcc
     8161 cgttgaagat ccggatgccg agaaccgccg acagtacgag gaacgcttcc actgccagca
     8221 gccatatacg tctttctctc aggaagaaca ccgcagtgct ggcgagaacg agatggatga
     8281 acaccagata gagaaaggtt ttgaggcgca gagtcacggc gtgattccgt acttttcaaa
     8341 acggcgatac aaggcaggcc ggctcagccc aagcgcttcg gcgaccttgc tgacgttgcc
     8401 gccgtaatgc tgcatgcact tgaggatcat ggattcctcc atttcatcga gcgtcattgc
     8461 ccccacagcg ggcaagccgt ccttctgcac ttcgcgggat tccattccca gggcaacagc
     8521 aaggtgatct acgtcgagga gatcgttccc cgttacaagc accgttcttt cgatcagttg
     8581 cttcaactgg cggacgtttc cgggccagct cattccctgg agccacttca aagccgagtc
     8641 gctgatcccg aggtcctgcc gccggtaaac gaggcctatg ctctcgagga aatgcctgac
     8701 aagcagcgga acgtcagcgg gtctttccct caacgcaggc agatgaatgg caatgaggtt
     8761 gagacggtaa agcagatctt cccggaaccc gccgctcttg acgagttccg gaaggttctg
     8821 attggtggcg gaaataaccc gaacatcgac cgcacgagtc gcgctggaac ccagcacttc
     8881 gtacgtcctg tcctgaagga cgcgcagaag cttgacctgg ctgttcggat ctagctcccc
     8941 gatttcgtca aggaagatcg tgccgccgtt cgcgatctca aaccgtccct tgcggtcctg
     9001 tcgcgcatcg gtgaatgctc ccttgacatg accgaacatt tcgctctcga agagcgtgga
     9061 tgagatcccg ccgaggttca ccttgacgaa cgcggcgttc ttccgtttgc tgttccgatg
     9121 aatcgcttcg gcgatgactt ccttgcccgt tccactttcg ccggtaatca agacggacgc
     9181 ttccgtggaa gcgatacggc cgatgacctc tagcaccttg agaagcttcg gatcacgccc
     9241 gacaaccatg cgaaagtcgt acagagagtc aagctcttcg cgggagagcg cccgctgtcc
     9301 ggcgcgcgca ttcgccgccg ccagtcctag cgctgtttcc acggactgga gaagctgttg
     9361 gttcgtccag ggcttggtaa tgaagtccgt cgcgcccgct ttcatgccct gaacagccag
     9421 ggaaatggaa ccccacgccg tgatcagaat caccggcatg tgcggccgtc tttccttgag
     9481 tgcctgaagc agagctagcc cttcctcgcc cgcggtcttt cgtgagaagt tcatgtcctg
     9541 gatcaccaga gcgaactcct cgagttccat ccgccgtacg gcctcattcg gagatcgggc
     9601 ggactgcgaa cgataacctg cttgcttcag caggagcgca agcgaagtcg taacagacgc
     9661 atcatcatcg acgatgagaa tcgccttatt catgaccttc ggccggttgt acaccggtca
     9721 ccgatcttga ttctactact cgtaatgcag cgcgccggcc ggttggacct tggttgcaag
     9781 ccagctcgga tacaggcccg cggcaatggt caggccgtac ataacaagga gcgaaacgat
     9841 cagtcctaca gtgaatacgc cgggactcat ccagtcgaca atttcgagaa gcggcagttg
     9901 cacgaccacg gccatgccga gcaacaatcc agcggtggcg atcaccagaa gctcgccaag
     9961 cacttgcctg tgaacatcgc cggcggttcc gccaagcgcc cgccgcagcc cgatttcctt
    10021 ggttcggcgg gagacgttct gccacaggac cccgaccatt ccgagtccga ccatgatgat
    10081 cagaaacacg gctacgagcg acgcagcagc catcggagcg agtacgagcc gcatgtggga
    10141 tttccgtgcg cgatcgagcg gctcgatctg aatcgtcctg tctttcatta ccgagcgcag
    10201 cctgttcatg agcttctcct cgaatgctgc cgtggttcct ggccgaagct ttacgagcaa
    10261 ggcaatcatt gccaagccag ataacttctt agcgctggct cggtggaagc agtagttgta
    10321 cagctcgtgg aattcgccgt gctggcggaa atcggtgatg actccgacga ccctttgcgg
    10381 catggttttt gcacgttctt ccggatcctt cgggagatcg aattccttgc cgagaggatt
    10441 ttcatggccg aacaactcgt cgcggagcct gacattgatg accacgggtt cataattcag
    10501 tgaatcatcc gacgagtcga accaccgtcc ttgaacgact ttcagttcga gaacatcttt
    10561 caattcatct gtcccgtcgt tatatcgggc ctcagcgctc cgtcccctat agcagatggg
    10621 accattactc gcgccgatct ggaacggcgt catcccgatg ccggcaactc cttctatttc
    10681 gtcgaggtcc cgcagagcca actgcatttg cttgaaatca ttcatagctt gggcacgttc
    10741 ggcatgacgg gaatcaaaaa ttccgcttac gctgacgttc cagacgtttt cgtaggagaa
    10801 acccaagggc cgcaggtaat tgtcggtgta gtaaataccg aaagccgtca ccccgaacaa
    10861 aaccaggaag gagaagaaga tctcggtgat gatcaggata ttggttcgct tacgattcca
    10921 taccagcttg aacatgtgtt ttatcatcgt gatgctcctc gcaacgcctc aacgggatgc
    10981 agccttgaca ttctccaggc aggatacacg ccggagaaca ggccaaagaa caacgtcatc
    11041 aacattccaa tgaggaagac ccgatagttg acctgaaact gtgcataggc aagaattccg
    11101 gcggcggtaa tgcctcgtag aacaacagcg gagaggacga agccgatcag ccctccgatc
    11161 agagtcagga gaacattctc catcacgaac tgtccgacca gcgtcaagga agatgcgcca
    11221 aacgtcttcc tgacaccgat ttccgatgca cgctccagga tccggcttac attgatattg
    11281 atgaggttaa cagtgggcaa caacatgaat aacaaggcat aaaagagcag ccacaaaacc
    11341 agacggctgg ttttctcccg cgcgtagtcg ct
//
LOCUS       DATIFQ010000381        14903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2043408, whole genome shotgun sequence.
ACCESSION   DATIFQ010000381 DATIFQ010000000
VERSION     DATIFQ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 14903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14903
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043408"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            464..1609
                     /locus_tag="VLE22_18475"
     CDS             464..1609
                     /locus_tag="VLE22_18475"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB16443.1"
                     /translation="MNRKTFLRSILSGAACFTTSCRPKPSISTRPAARKRDQELAERI
                     YEHTNAAYFVAATYIGDRLGLFKAMAGAGPLTAPQLAARTGLNNRYVLEWLRTMASSG
                     YVDYRPESGAFELPPEHVAVLVDEESPAFSTGLVEATVPDILMVPRVLAAFRTGKGIP
                     YGDYPPETFDAIERITKPDYQHLLTQQWLPAVPGIVEQLRAGGSAADLGAGAGHASLA
                     IAKAFPKAHAFGFEPYAPSVARARANAKASGLGDRVTFDVFDGIHVPGGPYQLITINY
                     SLHHAGDPAGLLRSSRRALATGGALLIVEYRKSARLEDDKDTLRAGFYGIGLLECLPT
                     ALAEGGPGYGTGIMEPDVRELAAKAGFRGFTRVRSDDPIRSLFVLRG"
     gene            complement(1828..3246)
                     /locus_tag="VLE22_18480"
     CDS             complement(1828..3246)
                     /locus_tag="VLE22_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16444.1"
                     /translation="MIEEARSTQEKALKINLDAVRFGTFAEIGAGQEVARWFFHVGKA
                     SSTVARSISAYDMAISDALYGPTQHYVSRARLEAMLDREFSQLVDQLNAKRGERNAFF
                     VFADTVATHGSSRSAGGHGWLGIRFQDKPGGEPSEVIIHIEMLDAFRASQQETVGLAG
                     VNLIYGAFSRHQDPAALIHSLMDGLDRRRIEIDMIKFTGPLFGGVDNRLMSLQLVEQG
                     LTDAALFTADGEVVQPSEVLHNRPVVIERGSFRPVTNVTLGMVDAALQQLRQDVDRPV
                     AEPVILMEMTLNNLMSAQTIDHQDFLARANILGALDKMVMISNYTRFDCVTTYLRQYT
                     QDRIGMAVGVPTLRAIFDAQYYTELKGDILEGLGRLFRGPVTLFAYPTVSAATRELET
                     ADKIGISPNLNHLYAYLFENGFIEPVRQFPTEQLHLNPGDVLRKIQGGDATWVDFVPA
                     KAAALIQRDGLFGCPPRAESPR"
     gene            complement(3396..4358)
                     /locus_tag="VLE22_18485"
     CDS             complement(3396..4358)
                     /locus_tag="VLE22_18485"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSB16445.1"
                     /translation="MNNRTSPAPDGAHELRTELARKIALFIGSAENRATDIPGLTLHR
                     RTAPTAPCSMTYEPSVTVIAQGRKRVDLGRTTFIYDASRFLLTSVDLPVVSRVIEASE
                     EAPCLALSLKLEMPVVRELLNREEIQVAEAPSDSPGMATGETTVEFLRACCRLVDLLD
                     TPRDIPFLSGLIQREIIYRILRSAEGARLRAIATLGEQSHRTAKAIAWIRANYAKPLR
                     VEDLAEIAGMGVSTLHHHFRVLTAMSPLQYQKQLRLQAARGRMLMDGLDAASAAFEVG
                     YESASQFNREYSRFFGQPPMRDIRILRSPSAPPLESVSNRQNAI"
     gene            4533..5525
                     /locus_tag="VLE22_18490"
     CDS             4533..5525
                     /locus_tag="VLE22_18490"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB16446.1"
                     /translation="MKKRKLGKSGLEVSALGLGCMGMSFGYGPAGEKKEMISLIRSAV
                     ERGVTFFDTAEVYGPFTNEELVGEALAPFREQVVIATKFGFKLDLNTGKQAGLDSRPE
                     HVKQVAEASLKRLKTNTIDLFYQHRVDPDVPIEDVAGAVKDLIQEGKVKHFGLSEAGV
                     KTIRRAHAVQPVAAVQSEYSLWWRSPEAEVLPTLEELGIGFVPFSPLGKGFLTGKIDE
                     NTTFDSSDFRNIVPRFTPEARKGNQALVDLLARVAEQKKATPAQIALAWLLARKPWIV
                     PIPGTTKLHRLEENIGAAAVELTSGDLREIDSAATQITVQGARYPENLERMTGR"
     gene            6018..6350
                     /locus_tag="VLE22_18495"
     CDS             6018..6350
                     /locus_tag="VLE22_18495"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSB16447.1"
                     /translation="MFEIIAEPNRRAILSLLVSSQQSVGEIERRLRMPQPAVSKHLRV
                     LREAGFVESTVDAQRRLYRLKPEPLQEVDAWLAPFRRFWSAHVDALERHLDRMDQSIP
                     TKRKARRV"
     gene            6351..6881
                     /locus_tag="VLE22_18500"
     CDS             6351..6881
                     /locus_tag="VLE22_18500"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSB16448.1"
                     /translation="MNDREQYEPGPARGAQIRKDGEKWTLILVRDLRHPPEKVWQALT
                     DPAHLREWAPFEADGSLGSVGTTVKLTWVGAPTPQETIVTRADAPKVLEYNDLRWQLE
                     AFGGGTRLTLWHSIDRRFISWGAAGWHICFDVLERLLAGEPIGRIVGAEAMNFGGWQR
                     LNAEYAKQFGVETPGW"
     gene            6923..7345
                     /locus_tag="VLE22_18505"
     CDS             6923..7345
                     /locus_tag="VLE22_18505"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSB16449.1"
                     /translation="MESTLQKPALVAARTASKATTIVYWIVTALFCLQMSFTAYAQLR
                     LPQVAEAFIHLGFPAYFRVELSCAKLLGVVLMLAPVPPRLKEWAYAGFAIDLASAVIA
                     HLSVGDGPEAWGWAAATGVLWGLSYFLWRRLQAAPASA"
     gene            7369..7923
                     /locus_tag="VLE22_18510"
     CDS             7369..7923
                     /locus_tag="VLE22_18510"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HSB16450.1"
                     /translation="MGLSVGVNTMAGKTSKKSANVAKKAAARRVAAKAAEPRKTAPKS
                     QFRRVAKPVLLAGGNPQIAKADGDTPVQAYIAAMPGWKRDVGRRLDALIVRTIPGVRK
                     AVRWNSPFYGIEGQGWFLDFHCFTNYVKVAFFRGMSLRPVPPGESKQKEVRYLDIHED
                     DELDEAQFAAWVEQASKLPGWGKS"
     gene            7937..8365
                     /locus_tag="VLE22_18515"
     CDS             7937..8365
                     /locus_tag="VLE22_18515"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HSB16451.1"
                     /translation="MKRPVPVESASTLIDGKIKELGDWRGKMLAKVREIIHEADPEIV
                     EEWKWIGTPVWSHGGIVCTGETYKNVVKMTFPKGAALEDPSGLFNSSLGGNVRRAFDI
                     HEGDKVDEAALKDLIRAAVALNLKGKGKPKPRRASSKRSG"
     gene            8487..8783
                     /locus_tag="VLE22_18520"
     CDS             8487..8783
                     /locus_tag="VLE22_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16452.1"
                     /translation="MRGVSRVRGHTVRPDGTLRDANSIPRGHWEAKDSRDYLAREIER
                     KIRRGYPLTNTIFEDTVSAVLYQNRDRRLLGQVIRVSLDTVRPVRSLPVAFSSA"
     gene            9046..9462
                     /locus_tag="VLE22_18525"
     CDS             9046..9462
                     /locus_tag="VLE22_18525"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HSB16453.1"
                     /translation="MDLRSDASELSLYKRLGGYEVIAAIVDDLFALLRADARFGRFGT
                     GRGIDSRKRAQQLTVDLICSLSGGPCYYIGRDMKTSHAGLGITESEWEANLELTRAAL
                     KKNAVGDREQAEFLSLFERYRHDIVDASESSAAGAE"
     gene            complement(9735..10514)
                     /locus_tag="VLE22_18530"
     CDS             complement(9735..10514)
                     /locus_tag="VLE22_18530"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB16454.1"
                     /translation="MAQQTTGQTQTPQAPTAQPPAPPKPRPAVPPEFDALPIWAMNAP
                     KLVAILKDPDSTVFQKAIACKKLSIVGGKEAIGPMAALLNHPQLACYARFGMEPNPDP
                     SVDEAFRAALPKLTGKLQIGVINSIGMRKDAKALDALKKLIDHSDPEVAQAAAASVGM
                     IGGLQASRVLQSALGRAKIPVFPVVARATLLCAEGLMATNRPRGLELYQDLSAPTMPK
                     PVRLAALRVLNAAGPAPEYTNEWPAPTGEAAAKDRSGFLGR"
     gene            complement(10539..11903)
                     /locus_tag="VLE22_18535"
     CDS             complement(10539..11903)
                     /locus_tag="VLE22_18535"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB16455.1"
                     /translation="MAEKQGKVTRRGFGKALATAAAASAPAAAQIATPGPGQFGPVVV
                     GNYRAVDQIVFGGIGIRGRGMADLRMLLGDNRVKFVGIADVREDAREMVKSTVDGYYQ
                     NNDCKMYRDPAEILARPDIDALLIATSDRWHGLMAMWAAQSGKDMYIEKPGAMSINEA
                     YAMADNVRRYGVVYQSGAQRKNQYAFEFAVGLARSGKLGRLTAVHADTAIGLGTVDAG
                     GHSWWAEESPEPDPLVFDWDKWLGPCLWRPYNSSYKDQGRGEFWDFHAGLLEWASHTV
                     AMAQWAADMEHTEPVEYIPEGPNFRGDGLIERYTIRCRYANGVQLILRNHSWRLLGSC
                     SNLFEGTEGWVEVGDSGRIEVSDNLKPLVPNKQGTAYDPTAEHLREFVNSIRSRTPPR
                     ANAEVTCHTHVASHAAWIAAQLNRRVEWDPVKRTFINDEQANRMRGRAYREPWRAEAL
                     ATSM"
     gene            12127..13443
                     /locus_tag="VLE22_18540"
     CDS             12127..13443
                     /locus_tag="VLE22_18540"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSB16456.1"
                     /translation="MIPKCLLSTAPGRAALRCAPILPFVLFPLAAAPLPAAKPEDVGM
                     SAERLQRIHEAIMRRVAAQDIPGAVTVVARRGRLVHFEAHGLMDVDAKKPMTKDALFR
                     LASTTKPVTAAAILILMEEGKLKLTDPVSKYIPEFKNSKVAMEWVPVPVPMTEGGPEA
                     GERYYTVPALHDITIVDLLTHTSGLATNGGITAPDFRKLQQARTPAETLADFIPRLGA
                     LPLDCQPGAQWRYSAYAGFDTLARIVEIASGSRFDRFLRQRIFEPLGMENTWFQVPEK
                     EKPRLVTVYRKTPSGLERSPERPGDVPAAYFGGASGLTSTAEDYLQFAQMLCNGGRLN
                     GKRILSPWTVDLMLSNHVGKLFEGGVHGRPPKGVGFGLGGEVVVNAVDARLRKPDGSY
                     GWSGAFGTYWWVNRKEQMAVVLFVQGPAGRSLEYDFDNAVSQAVIE"
     gene            complement(13920..14390)
                     /locus_tag="VLE22_18545"
     CDS             complement(13920..14390)
                     /locus_tag="VLE22_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16457.1"
                     /translation="MSSEVVCWISTEATTTRALAPSSSGAVSEIPSRFGQLIALASLG
                     RAEVEQKGKIGRLVLGSWQSAYLQWLCLDLKEQASDLRGYAEWLGVEVDETLEKQFLA
                     DLNLLVPQDSSAAERLLFLSDTEILFQLAGVRRRSGSVKLKQSMVGFPRSRFAH"
     gene            complement(14733..>14903)
                     /locus_tag="VLE22_18550"
     CDS             complement(14733..>14903)
                     /locus_tag="VLE22_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_067495976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HSB16458.1"
                     /translation="VAIVRKRLGLGISLYTFLQVLSVSLFEKTPILQAFQHTEDHNLS
                     PPSPNQLNLFHF"
BASE COUNT         2864 a         4661 c         4507 g         2871 t
ORIGIN      
        1 gcgatgccag agcgatgagc tgcccgaaac gagaagggat cccggagacg gtttcactca
       61 aggttggtgc cagtcgccgg gtcgtagtgg cttccgctga tatccaagag acaacttcgg
      121 aactcatggc acacccgaaa aagcgacggg ccgtgatgat aagtgaccca tgtaacacct
      181 tgggaggctc atccctcccc agtgacagct tcaagatata gtcttgctag aacataagcc
      241 aatagtacct ttaactcact tttggagtgc ggaagcgcgc agcgttcccc gccgccgggg
      301 ggagtcgtcc ctcccatcgc gcgctcggat tcgccggaaa agcgccaatc catccagaat
      361 gcacttgcca gcagcaggcg gatggccgga tagaacggag caacccacgc cgccgattcg
      421 gccgtcgtgc ggaaagtctc tgtatactga gccgaggcac ggcatgaatc gaaagacgtt
      481 tcttcggtca atcctcagtg gcgccgcttg cttcacgacg agctgtcgac ccaagccttc
      541 aatctcgacg cgccctgccg ctcggaaaag agatcaagaa cttgccgagc gcatctacga
      601 gcacacaaac gctgcctact ttgtggccgc tacctacatt ggcgatcgcc ttgggttatt
      661 taaagcgatg gctggcgctg ggccgctcac tgctccccag ttagcggcaa ggactgggct
      721 caataatcgc tacgtccttg agtggttgcg cacgatggcc tcgtcgggct atgtcgacta
      781 tcgccctgag tctggcgctt ttgagctgcc gcccgagcat gtcgcggtcc tcgtcgacga
      841 ggaatcaccc gcgttctcca cgggtctcgt cgaggcaacc gtgccagaca tcctcatggt
      901 cccgcgcgtc ttggctgctt tccgcacggg caaaggcatc ccttacggcg actacccgcc
      961 tgagactttc gacgcgatag aacgtattac gaagccggac taccagcacc tgctcacgca
     1021 gcaatggctg ccggctgtgc cggggatcgt cgagcagctt cgcgctggcg gcagcgccgc
     1081 cgatcttggc gccggcgctg gacatgcatc ccttgcgatt gccaaggcct ttcccaaagc
     1141 tcacgcattc gggttcgagc cttacgcgcc cagcgtggcg cgggcaaggg cgaatgccaa
     1201 ggcttcaggc ctcggagatc gtgtgacctt cgatgtcttt gatggcatcc atgtccccgg
     1261 cggaccgtac caattgatca cgatcaacta ttcactccac cacgccggcg accccgctgg
     1321 gctgctgcgc agctcgcggc gagcactcgc tacgggcggt gcgttactta tcgttgaata
     1381 ccgaaagtcc gcccgtctgg aggacgacaa agatactttg cgagccggct tctacggcat
     1441 cggattgctt gagtgcctgc caactgcgct tgccgaaggt ggacccggct acggcaccgg
     1501 tatcatggaa ccggatgtgc gcgaactcgc tgcaaaggct ggattccgcg gattcacgcg
     1561 ggtgagatcg gacgacccga tccgttcgct tttcgtactc cggggttgag gacgaatccc
     1621 gctgtaacag tcagccttaa cgcctcgacc tcggtcccgg aaccccgagc gacaaccggc
     1681 gttacctgct tggcggcgaa atcaaagaac tggatgaacc atttgccttc cggagcggca
     1741 acctccgctc gacagaggaa gtcatggggg tcgtcgacac acaagcggtc aaccgtccgt
     1801 actctacgac cttgcgacta acaggaatta gcgcggcgat tcggctcgtg gcgggcagcc
     1861 gaacagcccg tctcgctgaa tgagagccgc tgccttagcg ggtacgaagt ccacccaggt
     1921 cgcatcgccc ccttgtatct ttctcagcac atcgccagga ttaaggtgga gttgctctgt
     1981 agggaattgt cgcacgggct cgataaagcc attctcaaac aagtaggcat acagatggtt
     2041 caggttcggg ctgatgccga tcttgtcggc cgtttcgagt tctcgggttg cggcagatac
     2101 ggtggggtat gcgaaaagtg tgaccggtcc tctgaagagt ctgccgaggc cctcgaggat
     2161 gtcgcccttc agttcagtgt aatactgcgc atcgaagatc gcgcgcaggg tcggaacacc
     2221 gacggccatg ccaatccggt cttgtgtgta ctggcgcagg taagtggtga cgcagtcaaa
     2281 cctggtgtag tttgaaatca taaccatctt gtcaagtgcg ccgaggatgt tggcccgggc
     2341 aaggaagtcc tgatgatcga tcgtttgggc cgacattagg ttgttcagcg tcatttccat
     2401 gaggatgacg ggctccgcga ctggccgatc gacgtcttgc cggagctgct gcagcgcggc
     2461 gtcaaccatt cccagcgtga cattagtgac cggacgaaaa ctgccgcgtt cgatgacaac
     2521 cggccggttg tggaggacct cggatggctg aacaacctca ccgtcggcgg tgaacagcgc
     2581 cgcatccgta agcccctgtt ccacgagttg caggctcatt aagcggttgt ccactccgcc
     2641 gaacaggggg ccggtgaact tgatcatgtc aatctcaatc ctgcggcggt caagcccgtc
     2701 catgagactg tggatcaagg cagccggatc ctgatgccgg ctaaaggcgc cgtagataag
     2761 attcacgccg gcgaggccaa ccgtctcctg ctggctcgct ctgaaggcgt ccagcatctc
     2821 gatgtgaatg atgacttccg acggttctcc tcccggcttg tcctggaacc gaatcccaag
     2881 ccagccatgc ccgccggctg aacgcgatga gccgtgtgtg gccacggtat cggcgaaaac
     2941 gaagaatgcg ttgcgttccc cccgtttggc gtttagctga tcaacaagct ggctgaactc
     3001 gcgatccagc atcgcctcca gccgtgcccg gctcacatag tgttgggtcg gtccgtacag
     3061 cgcatcactg atcgccatgt cataagcgga aatcgatcgg gccacggtgg aagacgcttt
     3121 cccgacgtga aagaaccagc gggcgacttc ctgaccggct ccgatttccg cgaacgtgcc
     3181 gaatctcacg gcatccagat tgatcttcag agccttctct tgagtgctgc gtgcctcttc
     3241 aatcatccgc gccgctcccc gctggtacga ggccttcttt ggttcagcgt ctgtccctct
     3301 tgcactgaga ccatcttaac cagcatgacg cgctcgatcc taacggacag agtggattac
     3361 ttccggtttg ccggcgactc acccggaaca gacgatcaga tagcgttttg ccgattactg
     3421 accgactcca acggcggagc gcttggcgag cgaagaatcc gaatatcccg catcggtggt
     3481 tggccgaaga aacggctgta ttcacggttg aattggctgg cgctttcgta tccgacctcg
     3541 aaggccgcac ttgcggcgtc cagaccatcc atgagcatgc ggccccgcgc tgcctgcaac
     3601 cgaagctgtt tctgatactg aagaggactc atggcggtca gcacccggaa atgatggtgc
     3661 agcgtggaca cgcccatgcc cgcgatttct gcgagatctt ccacccgtag cggcttcgcg
     3721 tagttcgccc tgatccaagc gatcgctttt gccgttcggt ggctctgctc tcccagcgtt
     3781 gcgatcgctc gaaggcgtgc tccctccgca ctgcgaagga ttcggtagat gatctcgcgt
     3841 tgaatcaggc cactgagaaa gggaatgtcc cgcggcgtgt ccagcaggtc cacgagccgg
     3901 cagcatgcac ggaggaattc caccgtggtc tcaccggtcg ccatgccagg actgtcagac
     3961 ggcgcctcag ccacctgaat ctcctcccgg ttgagaagct ctcggaccac gggcatttca
     4021 agtttgagcg acaacgccag gcagggcgcc tcttcacttg cctcgatcac tcggctgacg
     4081 accggcaaat cgaccgacgt cagcaggaat cgcgacgcat cgtagatgaa agtggttcgg
     4141 ccgaggtcta cccgctttcg cccttgggcg atcacggtca cgctcggctc gtaggtcatt
     4201 gagcacggag cggtaggggc ggtccgtcgg tggagcgtca gcccgggaat atccgttgcc
     4261 cggttctctg cggagccaat gaacaaggca atcttgcgag ccagctccgt ccgcaattcg
     4321 tgtgccccat ccggtgccgg agaggtgcga ttgttcataa tgcccgttgc ttctcactcc
     4381 tctacaccag gatactgtct gcctccgtgg atgcaccagc aattcattaa gctacgagag
     4441 gaataggcaa taaatcgata ggatcaggat accgcctcca cggccctcga ccctaacctg
     4501 gagatgagag aagcgattac gaaggagaag caatgaagaa gcgcaaactt ggaaagagcg
     4561 gcttggaagt ctcggctctc gggctcggct gcatgggaat gagctttggc tacggtccgg
     4621 ctggcgagaa gaaggagatg atctccctga ttcggtcggc ggtcgaacgc ggcgtcacat
     4681 tcttcgatac cgccgaagtc tacggtccgt tcacgaacga agaactggtg ggcgaagccc
     4741 tcgctccctt ccgtgagcaa gtggtgatag cgaccaagtt cgggttcaaa cttgatctga
     4801 acaccggcaa acaggccggc ctggacagtc ggccggagca cgtcaagcag gtcgccgagg
     4861 cctcgctcaa acgtctcaag accaatacga tcgatctgtt ctatcagcac cgtgttgacc
     4921 cggacgtgcc gatcgaagac gtggcgggag cggtgaagga cctgattcag gaaggtaagg
     4981 tgaagcactt cggcctttct gaggctggag tgaaaacgat ccggcgcgca cacgccgtcc
     5041 agccggtcgc cgcagtccaa agcgaatact cgctgtggtg gcgctcgccc gaagcggaag
     5101 tgctgccaac ccttgaagaa ctcgggatcg gcttcgttcc gtttagccct ttgggtaaag
     5161 gcttcctgac ggggaagatc gacgagaaca cgacctttga cagctccgat ttccgcaaca
     5221 ttgttcctcg ctttacgccg gaggctcgga aagggaatca ggccttggtc gatctgctgg
     5281 ccagggtcgc agaacagaag aaggcgacac ctgctcagat tgcgctcgca tggctgcttg
     5341 cccggaagcc ctggattgtt ccgatccccg gcaccacgaa gctgcaccgg ctggaggaaa
     5401 acatcggagc cgccgctgtc gaactgacgt ccggcgatct gcgagagatc gacagcgccg
     5461 ccacacagat cacggtccaa ggagctcgat accccgaaaa cctcgagcga atgaccggcc
     5521 gctgagcggc cgccgagtga ggaatttagg gcctgaccac gggccgaaaa ggaaaccaat
     5581 aggagcagaa gctcgggacg ctcttcgcgc gcgccggacg agagcaaaac ccgggactga
     5641 ctccaaattt cgctgtgtct gcgaaattgg tgcctgtccc gggttctgcc atgaacgcgg
     5701 cccctgactt cgccgccttc gctgaagtcg gtcgcgaact cgcgcgcctc cacgtggact
     5761 gcgagcaact ggaaccttgg ccgctcgaat tcgtcgagaa tccttcggag cctctaacct
     5821 acaaggtcgt cggcttctcg attgtgctgg aaagtgttcg gcttcgtcga gagaaacttg
     5881 tccggagcgc ccagcgctct gaatcgcacc cgccaaaaac gcatcagacg gtgcaccccc
     5941 gcacggcgag gccccagtct ttaatatata ccttgacaag catatgctct atggagtata
     6001 taaagagcgt ggaatctgtg ttcgaaatca ttgcggagcc gaaccgccgc gcgatattga
     6061 gcctcctggt ctcgtcacaa cagtcggttg gagagatcga gcgtcggctt cgtatgccgc
     6121 agccggccgt gtcaaagcac ctgcgagtgc tgcgcgaggc cggtttcgtg gaatccacgg
     6181 tggacgcaca gcgccgtctc taccggctga aacctgaacc gctacaggag gtggatgcct
     6241 ggctggctcc gttccgccgg ttctggtccg ctcacgtgga tgctctcgaa cgccacctcg
     6301 accgcatgga tcaatcaata ccaacgaaga ggaaggcaag gagagtatag atgaacgatc
     6361 gcgagcagta cgaaccgggt ccggcccgcg gggcgcagat acgaaaggac ggagagaagt
     6421 ggacgctcat tctcgtcaga gacctgcgcc acccgccgga aaaagtctgg caggcgctaa
     6481 ccgacccggc gcatctgcgc gagtgggcgc cctttgaagc cgatgggagc ctgggttcgg
     6541 ttggaaccac ggtgaagctc acctgggtgg gagcgcccac gccgcaggaa acaatagtga
     6601 cgcgagccga cgctcccaag gtgcttgagt acaacgacct ccggtggcaa ctcgaagcct
     6661 tcggtggcgg cacgcgcctc acgctctggc acagcatcga tcgccgcttc atctcgtggg
     6721 gcgccgcggg ctggcacatc tgcttcgacg tccttgagcg cctcctcgcg ggtgagccga
     6781 ttggacgcat cgtcggcgcc gaagccatga acttcggcgg ctggcagcgg ctgaacgcgg
     6841 agtacgccaa gcagttcggc gttgagaccc ccggttggta gcccaacgcc ccgaagcgtt
     6901 gaagcgtagg aggaagaacc ccatggaatc gaccctgcag aagccggccc tcgttgccgc
     6961 caggaccgcc tccaaagcca cgaccatcgt ctattggatc gtcaccgcgc tcttctgcct
     7021 gcagatgagc ttcaccgcct acgcgcaact gcgcctgccg caggtggcgg aggcgttcat
     7081 ccacctcggc ttccccgcct acttccgggt ggagctctcg tgcgccaagc tcctcggcgt
     7141 ggtgctgatg cttgcgccgg tgccgccgcg gctcaaggag tgggcctacg ccggcttcgc
     7201 catcgacctc gcctcggcgg tcatagccca cctctcggtg ggcgacggcc cggaggcgtg
     7261 gggctgggcg gcggccaccg gcgtgctctg ggggctctcg tacttcctct ggcgccgcct
     7321 gcaggccgcg ccggcgagcg cctgaccgcg cagcgtcggc ggtttagtat gggtctatcc
     7381 gttggagtga acacaatggc cggtaagacg tctaagaagt cggcgaatgt cgcaaaaaag
     7441 gccgccgcca ggcgggtcgc tgcgaaggcg gccgaaccac gcaagacggc gccgaagtcg
     7501 cagtttcgca gggtcgcaaa gccggttctc ctcgcaggcg gcaaccctca gatcgcgaag
     7561 gccgacggcg acaccccagt gcaggcctac atcgcggcca tgccgggctg gaaacgcgac
     7621 gtcgggcgcc gcctcgacgc gctcatcgtg cgcaccatcc ccggcgtgcg caaggcggtc
     7681 agatggaact cgccgtttta cggaatcgaa ggccagggct ggttcctcga cttccattgc
     7741 ttcacgaact acgtcaaggt ggctttcttc cgcggcatgt ctctgcgtcc tgtccccccc
     7801 ggcgagtcca agcaaaagga agtgcgctac ctcgacatcc acgaggacga cgagcttgac
     7861 gaagctcaat tcgccgcttg ggtggagcaa gccagcaaat tgcccggctg gggcaagagc
     7921 tgagttggaa ctgtacatga aaaggcccgt cccggtggaa tcagcctcca cattaatcga
     7981 tgggaagatc aaggaacttg gggactggcg cgggaagatg ctggcgaaag tgcgcgaaat
     8041 cattcacgag gcagaccccg agatcgtcga agagtggaag tggattggga ctcccgtctg
     8101 gtcccacggc ggcatcgtct gcacgggaga gacgtacaag aacgtcgtca agatgacctt
     8161 tcccaaggga gctgcgttgg aggacccgtc aggtctgttc aactccagcc tcggcgggaa
     8221 tgtcaggcgc gccttcgaca tccacgaagg cgacaaagtc gatgaggcgg ccttgaagga
     8281 tctcatccgc gctgcagtgg cgctcaatct caagggcaaa ggcaagccga agccccggcg
     8341 agcgagcagc aagcggtccg gctagcttca tcagaatctg gcgaagctcg acgaaggcaa
     8401 agggccgcgc ttcgcaggcg tctgtgtgat gtcttgaaaa ttggtgtctg tcccaggttt
     8461 tgcccagtgg cacaggcctt cagcctgtgc gaggagtttc ccgcgtacgg ggccacaccg
     8521 tccgcccgga tggtaccctt cgcgacgcca acagcatccc ccgcggccac tgggaggcca
     8581 aggattcccg cgactacctc gctcgcgaga ttgagcgcaa gatccgccgc ggttacccgc
     8641 tcaccaacac catcttcgaa gacaccgtca gtgccgtcct ctaccagaac cgcgaccgcc
     8701 gcctcctcgg ccaggtcatc cgcgtcagcc tcgacaccgt ccgccccgtc cgctccctcc
     8761 ccgttgcgtt tagttctgcc tgacggcacg actcgggatc atcgtcatga acggcatgga
     8821 atgtcgaaaa gggcgaatgc cccggaagca cagggccaac ctcagagcgc gacgggcttt
     8881 atcttaggcg ctgcccatcc accgggaaaa gtgtagaaaa caagctgcat tcccatgccg
     8941 ggagatgggg aatccgctgc gagccggcta gaggatgcca catcaacggg atggcgactt
     9001 tgcggccaat ggactgtggg cacatcggcg cgcataatcg accgtatgga ccttcgcagc
     9061 gacgcgtcag aactctcgtt gtacaagcgg ctcggcgggt atgaggtgat tgcggcaatt
     9121 gtcgatgacc tttttgccct actccgggca gatgctcggt tcggccgctt cggcacgggt
     9181 cgcggcatcg attcacgtaa gcgggcgcaa cagttgaccg tggacctgat ttgctcgttg
     9241 tcaggcggac cgtgctacta cattggccgc gatatgaaaa cctcacatgc cggtttggga
     9301 attaccgaat cggagtggga agccaatctc gaactcacca gggcggcgct taagaagaat
     9361 gctgtgggtg atcgagagca ggcggagttc ttgtctctgt ttgaacgcta caggcacgat
     9421 attgtcgacg cttctgaatc gtccgcggct ggcgcagaat gacggcccct ccatcgacgg
     9481 ttcgcagccg acccgtagat gtggatgccg gtcatggtcg tgcgcggttg acggcctata
     9541 actccaggtt cacctgacct ctcgcccatg tggattacgt ccgcattgcc ggcgatacac
     9601 tcgcggccga gtccgctgtg gggaggcgcg caacgccagt tcaccgggcc attgtcggcg
     9661 tccgccccaa tcccccatac atggtgtgga gcgagggggc ggccatccgg atcgaatatt
     9721 cgctttgttc taccttacct gcccaggaac ccgctcctgt ccttcgctgc cgcttctcct
     9781 gtcggcgctg gccattcatt cgtgtactcc ggtgccggac ccgccgcgtt caacacccgc
     9841 agagcggcca gccgcaccgg cttcggcatc gtcggcgcgc tcagatcctg gtacagctcg
     9901 agcccgcgcg ggcgattggt ggccatcagg ccttcggcgc acagtagcgt cgcccgtgcg
     9961 actactggga ataccgggat tttcgcccgt cccagggccg attgcagtac cctcgaagcc
    10021 tgcaggccgc caatcattcc caccgacgcc gccgcggctt gcgccacctc agggtcgctg
    10081 tggtcgatca gcttcttcag cgcgtctagc gctttcgcgt ccttgcgcat gccgatgctg
    10141 ttgatcacgc cgatctgcaa tttccccgtc agcttcggca acgctgcacg gaaggcttcg
    10201 tctaccgagg ggtcgggatt cggctccatg ccgaaccgcg cgtagcaggc gagttgcgga
    10261 tgattgagca acgcggccat cggcccgatg gcctctttgc cgcccacgat cgagagtttc
    10321 ttgcacgcaa tcgccttctg aaacacggtc gagtccgggt ccttcaggat cgccaccagc
    10381 ttcggcgcgt tcattgccca gatcggcagc gcgtcgaact ccggcggaac cgcgggacgc
    10441 ggctttgggg gcgcgggcgg ctgtgccgtc ggggcctggg gggtctgggt ctgcccggtc
    10501 gtctgttgtg ccattgcgat gatctccttt ccgcgttcct acatgctcgt cgccagggct
    10561 tccgcccgcc agggttcccg gtaggcgcgg ccgcgcatgc ggttggcctg ctcgtcgttg
    10621 atgaacgtcc gtttcaccgg gtcccactcg actctccggt tcagctgcgc cgcaatccac
    10681 gccgcgtgag atgccacgtg cgtgtggcac gtcacttcgg cattcgcacg cggaggagtg
    10741 cgcgaccgga tgctgttgac gaactcgcgg aggtgctcgg ccgtcgggtc ataagctgtg
    10801 ccctgcttgt tcggcaccag cggcttcagg ttgtccgaca cctcaatccg cccgctgtcc
    10861 ccgacttcga cccagccctc cgttccttca aacaggttgc tgcacgatcc tagcagccgc
    10921 caactgtggt tccgtaggat cagttgcacg ccgttggcgt accggcaccg gatcgtgtac
    10981 cgctcgatca ggccgtcgcc gcggaagttt ggtccttccg gaatgtactc gaccggctcg
    11041 gtgtgctcca tgtcggctgc ccactgcgcc atcgccaccg tgtgcgacgc ccattcgaga
    11101 agtcccgcat ggaaatccca gaactcgccg cggccttgat ccttgtagct cgagttgtac
    11161 ggccgccaca ggcagggccc cagccactta tcccagtcga atacaagcgg atccggctcc
    11221 ggactctctt cggcccacca gctgtgcccg ccggcgtcca ccgtaccaag tccgatggcc
    11281 gtgtccgcgt gtaccgcggt cagccgcccc agcttgccgc tccgggccag ccccaccgcg
    11341 aactcgaacg cgtattggtt cttcctctgt gcccctgact ggtagaccac gccgtaccgc
    11401 ctcacgttgt ccgccatcgc gtaggcttcg ttgatcgaca tggcgccggg cttttcgatg
    11461 tacatgtcct tgcccgactg cgccgcccac atcgccatca acccgtgcca ccggtcgctg
    11521 gtcgcgatca gcaacgcgtc gatgtccggg cgcgccagga tctccgccgg gtcgcggtac
    11581 attttgcagt cgttgttctg gtagtacccg tcgaccgtgc tcttcaccat ctcgcgcgcg
    11641 tcctcgcgaa cgtcggcgat tccaacgaac ttcactctgt tatcgcccag cagcatccgg
    11701 aggtccgcca tcccgcgccc gcgaataccg ataccgccga agacaatctg gtcgactgct
    11761 ctgtaattgc ccactactac cggcccgaat tggccaggcc cgggtgtggc gatttgcgcc
    11821 gcggccggtg ccgatgccgc tgccgccgtc gccagcgcct ttccgaatcc ccggcgcgtg
    11881 acctttcctt gcttctccgc cattgctctt tctccctcgt tatggtcaag ctatgccaca
    11941 gtacattagc tgaacaaacc tcccgcggga gcgcaactcc gccgatcgtg cgaggtcagg
    12001 tcttgaccac ccggagcacg agttcgcccg ctcggcgtgg gcgcccccgt ggcccacagc
    12061 ggcggtggcg tactgttgca gcggaccaat cttggatcgg atgggctctt gtgacaaagt
    12121 ggagccatga ttcccaaatg cctgctttcg actgctcccg gccgcgccgc gcttcggtgc
    12181 gcgccgattc tgccgtttgt tcttttcccg ctcgcggccg cgccgctgcc ggcggcgaaa
    12241 ccggaggacg tcgggatgtc cgcggaacgg ctgcagcgca ttcacgaggc gatcatgcgg
    12301 cgcgtggcgg cgcaggacat acccggagcc gtgaccgtgg ttgcgcggag ggggcgtctg
    12361 gtccatttcg aggcacacgg gttgatggat gttgacgcga aaaagccgat gaccaaggac
    12421 gcgctgttcc gcctggcgtc gacgacgaag ccggtgacgg cggccgcgat cttgatcctg
    12481 atggaagaag gcaagctgaa gctgaccgac ccggtttcga aatacatccc ggaattcaag
    12541 aactccaaag ttgcgatgga atgggtaccc gtcccggtgc ccatgacgga aggcgggccg
    12601 gaagccgggg agcgatacta cactgttccg gcgcttcacg acatcaccat cgtcgatctg
    12661 ctgacgcaca cctccggact cgctacgaac ggcggaatca cggcgccgga tttccggaag
    12721 ctgcagcaag cccgcacgcc ggccgaaacg ctggcggatt tcattccccg gctgggagca
    12781 ttgcccctgg attgccagcc cggcgcccag tggcggtaca gcgcatacgc gggcttcgac
    12841 acgctggccc ggatcgtcga gatcgcctcg ggatccaggt tcgaccgctt cctccggcag
    12901 cgcatcttcg agccgctcgg aatggagaat acgtggttcc aagttccgga gaaggagaag
    12961 ccgcggctcg tcacggtgta ccgcaagacc ccgagtggat tggagagatc gcccgagcgg
    13021 cccggagacg tgccggccgc ctattttggc ggcgcatccg gcctcacgtc aaccgccgaa
    13081 gactacctgc aattcgcgca gatgctctgc aacggcgggc gactgaacgg aaagcgcatt
    13141 ctcagtccgt ggacggtcga cctgatgctg agcaaccacg tcggcaagct tttcgagggc
    13201 ggcgtgcacg ggcgcccgcc gaagggcgtc gggttcggcc tcgggggcga ggtggtggta
    13261 aatgccgtgg acgcgcggct gcgaaagccg gacggcagtt acggctggtc cggcgcgttc
    13321 ggcacgtact ggtgggtgaa ccggaaagaa cagatggcgg tggtgctctt tgtgcaaggg
    13381 ccggcggggc ggtcgctcga gtacgacttc gacaacgccg tgtcccaggc cgtaatcgaa
    13441 tagtgccggg ccgacacttc gtagggccgc tcctctggaa aaacggactg gctccaattt
    13501 cgtggtgttt gcgaaattgg tgcctgtccc cgcttttccg cctgtccttg cgccagcgcc
    13561 tcgcgtgcgc ggtcagaaac ctgcccccat gttcgaggca cttttgacca gccggggaca
    13621 tgggtaacac tttagaccgg cgacatgggt gacagttgcg cgaagcgcga ccgtcaggga
    13681 gcggaagcgc aatggaaccg cgcgcgtaag caagcggtac tttgtacgcc gggctccctg
    13741 gcccggctag gatctgccgg ctttcaatcc cgctccaccc accttccgct ccaacctcat
    13801 cccccgatat tcactctgac gtgaccccag cgcaccccac accctccctc cgcgatacaa
    13861 tcgtgacgga aaatcgggtc ggatgcgccg gaagtcaggg cacccggcgc tcgtggacct
    13921 caatgagcga accgcgatcg tgggaagcca accatggatt gcttgagctt cacagatccg
    13981 ctgcgccggc gaactccggc aagctgaaac agtatctccg tgtcgctcaa gaacaacaac
    14041 ctctcggctg ctgacgagtc ttgcggtacg agcaagttca aatccgctag aaattgcttc
    14101 tccagcgtct cgtcaacttc gacacccagc cactcggcat aaccacgaag gtcggatgcc
    14161 tgctccttca agtccaggca cagccactga agataggcgg attgccagct cccaagaacc
    14221 aacctgccga tcttcccctt ctgctcaact tcagcccgcc cgagcgatgc cagagcgatg
    14281 agctgcccga aacgggaagg aatctcggag acggctccgc tcgagcttgg ggccagtgcc
    14341 cgggtcgtag tagcttcggt tgatatccaa caaacaactt cggaactcat ggaacacccg
    14401 aaaaggtgac gggccgcgat gacaagctgc ccgtttgcca ccttaagaag tacatcccga
    14461 cgcaggggac agcttcaaga tatagtcttg ctagtacata agccaatagt acctttaact
    14521 caattttgga gtgcgacggc gtgcagcatt ccccgctttt ccgcctgtcc ttgcgccagc
    14581 gtctcgcgtg cgcggtcaga aacctgcccc atgttcgcat gcagttacgg aagcgcaagt
    14641 cgccagactt gccgcaccgg tcgttgtgta gcaaaacccc ggattgactc gaagtttctg
    14701 aagaaatggg ggactgaccc aggagtgtcc ggttaaaagt ggaagagatt caactggtta
    14761 ggggagggcg gtgaaaggtt gtgatcttcg gtgtgttgga aggcctgtaa aatcggggtt
    14821 ttctcgaata agctgacgct gagaacttgc agaaacgtgt agaggctgat gcccaggccc
    14881 agtcgtttgc ggacgatggc gac
//
LOCUS       DATIFQ010000382         7193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1850978, whole genome shotgun sequence.
ACCESSION   DATIFQ010000382 DATIFQ010000000
VERSION     DATIFQ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850978"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..862
                     /locus_tag="VLE22_18555"
     CDS             59..862
                     /locus_tag="VLE22_18555"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16459.1"
                     /translation="MRNHIIQVLPAIAVAMLSLPVPAAAQAQGANKEKVKTYMPFHQY
                     TQAENEKILKLYEELRVSDVSDGLDAVGLADVGIMEPDIKTLWRDTDNYTHRIIGIAV
                     TARYVPSNKREAKMPDETVGRWYQELTSEAFMDALHPNSVLVIDGNQDGDTRSIGSSN
                     IKAWQKRGMLGVVTSGGVGDTDEIIHQRIPVYYKRLGRGLRPGRNELESVNRPVMCGG
                     VLVRPGDIVVGDGDGVVVVPRERAEEVANAARPYLDNIGLERWKKKQQK"
     gene            894..1730
                     /locus_tag="VLE22_18560"
     CDS             894..1730
                     /locus_tag="VLE22_18560"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB16460.1"
                     /translation="MASSILSRRECLAGAAGLAALRGFGADASAEFAHDDIKLGVASY
                     SLRKFPRARAIEMVRSLRTPYICIKEFHLAYSSTPEELAAARIECESAGLQILSGGVI
                     YLEKQEDLRRMFDYAKGAGMPMMICAPKRPLVGEVEKLVKEYNIRAAIHNHGPEDKEF
                     PTPQSVLEAIRGTDPRMGLCMDIGHTARAGQDVLEWIDKAGKRLFDIHAKDLVDTSRN
                     ARNVPVGEGILPVAAMLRLLKKIDYKDGVMLEYEIDEDNPLPGMRESFAYMRGVLAGL
                     RG"
     gene            complement(1799..3904)
                     /gene="pnp"
                     /locus_tag="VLE22_18565"
     CDS             complement(1799..3904)
                     /gene="pnp"
                     /locus_tag="VLE22_18565"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HSB16461.1"
                     /translation="MVHRTEIDLGGRKISLETGKVAKQANGAVVVRSGDSVVLVTACA
                     NEEPKPGASFFPLTVDYREYTYAAGRFPGGFIKREGRPTEKEILTSRLIDRPIRPLFP
                     EGFSCETQVIGLVLSADPELDPGPLAIVGAAAALAISDIPFHHVLGGLRVAMVNGELI
                     ANPTYSETREAKLNIMVAATDDGIVMVEAGALQATEAEVLAAIEFGHQSCRKITTAIR
                     ELVAAVGKTKRLFEPPAINQEMYDQIAAQHRAELSDALNTEKYPKLESYSKVKALRAK
                     AVEAYPEEQQAEAGDLFDRLKERVFRDEMLKDRRRPDGRAFDQIRKIDIEVGPLPRTH
                     GSALFTRGETQSLVTTTLGTKDDEQRLELLESGETSKRFMLHYNFPPFSVGEVAFLRG
                     AGRREIGHGALAERSLLPVIPDEKTFPYTMRVVSDILESNGSSSMATVCGASMALMDA
                     GAPIVAPVAGIAMGLVKEGDDFAILTDIAGAEDHYGDMDFKVAGTREGITALQMDIKV
                     QNITTKLMEQALEQARQARLFILDKMHQVLESPRAALSPYAPRIYTLTIPQDKIRELI
                     GPGGKVIRGIIEQTGVKIDVEDDGTVNIFATDGASADKAIQMVTDIAAVAEVGKTYLG
                     TVVRLVDFGAFVEIFPGTDGLLHISEISENRIRNVRDELKEGDQILVKVLALEGNKIR
                     LSRKAVLREQREKLKTGKS"
     gene            complement(4126..4395)
                     /gene="rpsO"
                     /locus_tag="VLE22_18570"
     CDS             complement(4126..4395)
                     /gene="rpsO"
                     /locus_tag="VLE22_18570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSB16462.1"
                     /translation="MALAADVKSETIGKFRQHQSDTGSPEVQIALLSRRIAELTEHFK
                     SHQKDHHSRRGLLTMVAQRRRLLKYLKDRSPERYQTLIQELGIRR"
     gene            4482..5060
                     /locus_tag="VLE22_18575"
     CDS             4482..5060
                     /locus_tag="VLE22_18575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB16463.1"
                     /translation="MARKVAAGFEFHPVTSARWPDMEKLFGARGACGGCWCMWWRLPR
                     QEFVQGKGEDNKRAMGRLVAAGGTPGILAYSGGEPVGWCSVAPRESFPVLDRSRILKR
                     VDDQPVWSVVCFFVAKKHRRQGLAVKLLEAAAAYAEKRGARIVEGYPVEPKKGDMPDV
                     FVYTGLASAFRKAGFVEVARRSETRPVMRRVL"
     gene            5469..7013
                     /locus_tag="VLE22_18580"
     CDS             5469..7013
                     /locus_tag="VLE22_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16464.1"
                     /translation="MSNIRYMAHGRLNSPILKALVCSGSVYLVPVSFYDFGARFWCSV
                     LLSPRLDDAFAATVWIAYLAWTVVLQGLLAVGLYFFFKHATRLMWAPITAAALFAAIG
                     VPLSIVAVWLDARVVPFIYREGEGEAVDWEESCRAEHAHLLVPARSRYGFLERSGRAW
                     IVPHAVEEPTLLLPEGCQKVAYTASETGENELRRIFKRESGFDIGWHHAGFRYMWFDK
                     HVSSNQGTTWSSEYGYQFTPTGVMFQRQYLPGGCVIFGLSPLGKRDVPAKSGAGCPVL
                     SGDSDSLGSLERAAYSVSERRVDIGSPGDGTELVVDISKVGPGFISLEGVSVERRRLL
                     IRRQSSRLLNVKIADLEDRGFSHEYSEISFDGQVAWRLPRVGDADDARLVADSPNWIS
                     WQTEPLRAPGCCVLEWNVGGRVGTHKVARGRNIVSVSLNPTGKLIAVSVSDMHRTGEI
                     RDSVYVIRSRDRAEVFRRYLPPLTQSRVQFVGPSLLAYTDFDGSDGFVRVVKVPENID
                     IANPGS"
BASE COUNT         1355 a         2087 c         2284 g         1467 t
ORIGIN      
        1 gggccctttt gttcgcggcg cactcaaggt aaagtagagg gcttcaggag agacgatcat
       61 gcgaaatcac atcatccagg ttctccccgc gatcgcggtc gcgatgctca gccttccggt
      121 gcctgccgcg gcgcaggcgc aaggcgccaa caaagagaaa gtcaagacct acatgccgtt
      181 ccaccagtac acccaggcag agaacgagaa aatcctcaag ctgtacgagg aattgcgggt
      241 gtctgatgtc tccgacggcc tggacgcagt cggcctcgcc gatgtcggga tcatggagcc
      301 tgacatcaaa accctttggc gggacacgga caactatacg caccggatca tcggcatcgc
      361 agtgaccgcg cgctacgttc cctccaacaa gcgcgaggcg aaaatgccgg atgaaaccgt
      421 cgggcgatgg taccaggaac tcacgtcaga agccttcatg gatgctcttc atccgaattc
      481 ggtgctcgtc attgacggca accaggacgg tgacacgcgt tcgatcggct ccagcaacat
      541 caaggcctgg cagaagcggg gcatgctcgg cgtcgtgacg agtggtggag tgggcgacac
      601 ggatgagatc atccaccagc ggattccggt ttactataaa cgtctcggcc gcgggcttcg
      661 gcccgggagg aatgaactcg agtcggtgaa ccggccagtg atgtgcggcg gcgtgctggt
      721 tcgtccgggg gacatcgtgg tcggcgacgg cgacggcgtg gtcgtggtcc cccgcgaacg
      781 ggccgaagag gtcgcaaacg ccgcccggcc atatcttgac aatatcggcc tcgaacgctg
      841 gaagaagaaa cagcagaaat agcagccaaa atttcggaag agagggtcaa ccgatggcat
      901 catcgatact gagccgccgg gagtgcctgg ctggggcggc ggggcttgct gcgctgcgtg
      961 gattcggcgc ggacgcgtcg gcggaattcg cccacgacga catcaaactg ggggtggcca
     1021 gttactccct gcggaagttt ccccgtgcgc gggcgatcga gatggtccgc agcctgcgca
     1081 cgccttacat ctgcatcaag gagttccact tggcgtacag cagcacgccc gaggaactgg
     1141 cggcggcgcg gatagagtgt gagagcgccg gcctccagat cctgagcggc ggcgtgatct
     1201 acctcgaaaa acaggaagac ctccggcgca tgttcgacta cgctaagggc gccggcatgc
     1261 cgatgatgat ctgtgcaccg aagcggcctc tggtcgggga ggtcgagaag ctcgtcaagg
     1321 agtacaatat ccgcgcggcg attcacaacc acggccccga ggacaaagag tttccgacac
     1381 cgcagagcgt gctggaagca atccgcggga cggacccacg catgggcctc tgcatggata
     1441 tcggccacac cgctcgggca ggtcaagatg tcctcgagtg gatcgacaag gcgggtaagc
     1501 ggctgttcga tatccacgcg aaggacttgg tcgacacaag ccgcaacgcc cggaacgtac
     1561 cggtgggaga aggcatcctg ccggtggccg cgatgttgcg actgctcaag aagatcgact
     1621 acaaggatgg cgtgatgctt gagtacgaaa ttgacgaaga caacccactg ccgggaatgc
     1681 gggaatcgtt cgcctacatg cgcggggtac tggcgggact gcgcggatag cttccgtggt
     1741 ccgcggcttg tagcgcgtga ctcctccgcg cgctacaagc catagtccac gagcttttct
     1801 aggactttcc ggtcttcagc ttttcgcgct gctcacgtaa caccgctttg cggctgagac
     1861 ggatcttgtt gccctccaat gcgaggacct tcaccaggat ctgatcgcct tccttcagtt
     1921 cgtcgcggac gttccgaatc cggttctccg agatctcgct gatgtgcagt aggccgtcgg
     1981 tcccggggaa gatctcgacg aaagcgccga aatccaccag ccggaccaca gtgccaaggt
     2041 acgtcttgcc gacctcggcg actgcggcga tgtcggtcac catctgaata gccttgtcgg
     2101 cggaggcgcc gtcggtggcg aagatgttca ccgtgccgtc gtcctctacg tcgatcttga
     2161 cgccggtctg ctcgatgatg ccacggatga ccttcccgcc cggtccgatc aattcgcgga
     2221 tcttgtcttg cgggatggtc aaggtgtaaa tgcgcggggc ataaggtgac agggccgctc
     2281 tgggagactc gagcacctgg tgcatcttgt cgaggataaa gagcctcgct tgccgggcct
     2341 gctcaagcgc ctgctccatg agcttggtgg ttatgttctg aaccttgatg tccatctgga
     2401 gggcggtgat gccctcgcgg gttccggcga ctttgaagtc catgtcaccg tagtggtcct
     2461 cggcgcccgc gatgtccgtc aggatggcga agtcgtcacc ctctttgacc aggcccatgg
     2521 cgatgccggc caccggcgca acgatcggcg cccctgcgtc catcaaagcc atgctcgcgc
     2581 cgcagacggt cgccatcgaa ctcgaaccgt tggactcgag gatgtcactc actacccgca
     2641 tcgtgtaagg gaacgtcttc tcgtcgggaa tcacgggaag cagagatcgt tcggccagcg
     2701 ctccgtggcc gatttcacgg cggccggcgc cacgcaggaa ggccacttca cccacgctga
     2761 acggcgggaa gttgtagtgc agcatgaaac gcttggaagt ctcgccggac tccagcagtt
     2821 ccagtctctg ttcgtcgtcc ttcgtcccaa gggtagtcgt aaccagcgac tgggtctcgc
     2881 cgcgggtgaa caaggcggag ccgtgcgtgc gaggcagcgg gccaacctca atgtcgatct
     2941 ttcggatctg atcgaaggcc cggccgtcgg gacggcggcg atctttcagc atctcgtcgc
     3001 ggaaaacgcg ctctttcaag cgatcgaaca gatcgcctgc ctcggcctgt tgttcctcgg
     3061 ggtaggcttc cactgctttg gcgcgcagcg ctttcacttt cgagtaactc tcgagcttcg
     3121 ggtacttctc ggtattcagc gcgtcgctca gctcggcgcg gtgttgggca gcgatctggt
     3181 cgtacatctc ctggttaatc gcgggcggct cgaacagccg cttggtcttg cccacggccg
     3241 cgaccagctc tcgaatggcg gtcgtgatct tgcggcacga ctggtggccg aactcgatgg
     3301 cggcgagcac ctccgcctcg gtcgcctgca aggcgccggc ttcaaccatc acgattccgt
     3361 cgtcagtggc ggcgaccatg atgttcagct tcgcctcgcg ggtctccgag tatgtcgggt
     3421 tggcgatcaa ttcgccgttt accatcgcga cgcggagacc gcccagaacg tggtggaacg
     3481 gtatgtcgga aatggccagc gcggcggcag cgccgacgat cgccagcggt ccggggtcga
     3541 gttcgggatc ggcagagagc accagaccga tcacctgggt ctcgcaggaa aaaccttcgg
     3601 gaaagagggg acggatagga cggtcgatca gacggctcgt caggatctcc ttctccgtgg
     3661 gacgtccctc gcgcttgatg aagccgcccg gaaaacggcc ggcggcgtac gtgtactcac
     3721 ggtagtccac cgtgaggggg aaaaaggagg cgccgggctt aggttcctcg ttggcgcagg
     3781 cggtgaccag caccaccgaa tcgccggaac ggacgacaac cgcgccgttg gcctgttttg
     3841 cgactttgcc tgtttccagg ctgatttttc gaccacctag gtcgatttct gttctatgta
     3901 ccatgggatt ctccaacctc agccgatttt cggcatgcgg tgtctcagac cggcgtcact
     3961 cgccggctta cgtgtgagac tggagttgac aatggtggga gtgacgtccc ttgccgggac
     4021 gtcgcttggc gggtccacta ccccagcgat ttctaacttc cccagtggtg aagaatcttc
     4081 gtatcgcaat gggctttcgg cccgcccctg ccttgggagc ccgccctagc ggcggatgcc
     4141 gagttcctga atcagcgtct gataccgttc cggactgcga tccttcaggt atttcagaag
     4201 ccgtcggcgc tgagcaacca tggtcagcag gcctctccgc gagtggtggt ccttttgatg
     4261 ggacttgaaa tgctcggtga gttccgcgat tcgccggctc aagagagcga tctgcacctc
     4321 gggcgagcca gtatccgact ggtgctgccg aaacttcccg atggtctcgc ttttgacgtc
     4381 tgccgccaat gccatgctag ggtacaaact ccttgtcttc tctaaatctc gtttttgtac
     4441 catcttagaa tagcacaaac aggagggccg gaatccgcgg tatggccagg aaagtggcgg
     4501 caggtttcga gttccatcca gtaacctccg caaggtggcc cgacatggag aagcttttcg
     4561 gcgcccgagg cgcctgcggt ggctgctggt gtatgtggtg gaggctcccc cggcaagagt
     4621 tcgtccaagg caaaggcgaa gacaacaagc gtgccatggg acgactcgtg gccgcgggcg
     4681 ggacgcctgg catcctggcc tattccggcg gcgagcccgt ggggtggtgt tcggtggcgc
     4741 ctcgggagtc gtttccggta ctcgaccggt cccggattct gaaaagggtg gacgaccagc
     4801 cggtatggtc ggtcgtctgc tttttcgttg cgaagaagca ccgtcggcag ggactcgcgg
     4861 tgaagttgct cgaagcagcg gccgcatacg cggagaagcg cggcgctcgg atcgtcgagg
     4921 gctatcctgt cgagccgaag aagggcgaca tgcccgatgt cttcgtctat actggactgg
     4981 cctcggcgtt ccggaaagcc ggctttgtcg aggtggcgcg gcgctcagaa acgcggccgg
     5041 tgatgcgacg ggtgctgtag catccaccct actgcttgat gaccgtgccg tcgcggcggc
     5101 ggacttcgcc ccagcagggt gcctcactcg tggagaggcg gaggccggat tcgtatgcag
     5161 agagcgatgt cgcgtaggcc gcggcctacc cgcagtacgt ggaatccgcg aactgcgaga
     5221 cttgtccttc atcctgcacc tcttggtggg ctgaacggcc cttgcgcagg caaatagcga
     5281 ctgctctaaa cggagttggt tgcgtgctcc aaatgattat gccgaggttt gctgtcgggc
     5341 gcgtaccgtt cggccgacgg cgattcctct atgccagctt tccgaatccg gagtgggaag
     5401 tccgctagaa ttggcgcttg cggcaccccg gaccgacgga tcccgtcgga acacgagatc
     5461 accggttggt gtcaaacata cggtacatgg cacacggcag gttgaattct cccatactga
     5521 aggcgttggt gtgttccggt tcggtctatt tggttccggt gagtttctac gacttcggag
     5581 ccagattctg gtgctcggta ctcctatccc ccagactaga tgacgccttt gcggcgactg
     5641 tctggatcgc ctaccttgcc tggacggtcg tgctacaggg ccttctcgct gtcggattgt
     5701 atttcttctt caaacacgca actcgactaa tgtgggcgcc aattactgct gcggctctat
     5761 ttgctgctat cggagtaccg cttagcatcg ttgccgtgtg gctggacgcc cgcgtggtgc
     5821 cgttcatcta ccgcgagggc gagggcgaag ccgttgactg ggaggaatcc tgccgagccg
     5881 aacacgcaca tctcctcgtg ccagcgcggt cgcgctacgg ctttctcgaa cggtcaggtc
     5941 gggcatggat agtcccccac gcggtggaag agccgacgct tcttctgcct gagggctgtc
     6001 agaaagttgc atatacggca agcgaaacgg gggagaatga gctaagaagg atcttcaagc
     6061 gtgaatccgg gttcgacatt ggatggcacc atgcaggctt tagatacatg tggttcgaca
     6121 aacacgtatc ctcgaatcag ggcacgactt ggagttcaga gtacggctat caattcacac
     6181 cgacaggcgt gatgttccag cggcagtacc taccgggagg atgtgtcatt ttcgggttaa
     6241 gcccgctcgg aaagagagac gttccagcca agagtggtgc cgggtgtccc gtactttcgg
     6301 gggactctga ctcactgggg tcactcgagc gcgcagcata cagcgtgagt gaacgacgcg
     6361 tggacatcgg gtcgcctggc gatggtacgg aactcgtggt cgacatttcc aaggttgggc
     6421 cgggcttcat tagtcttgag ggagtctccg tggaacgccg caggcttctt atcagacggc
     6481 agtcgagcag gttgctaaac gttaagatcg ccgacctgga ggaccgcggt ttctcccatg
     6541 agtactcgga gatctccttc gatggccaag tggcatggcg gttacctcgg gtcggcgatg
     6601 cggatgacgc acgactcgtc gctgactcgc cgaactggat ctcgtggcaa accgaaccac
     6661 tacgagcgcc cggttgttgc gtgttggagt ggaacgtggg agggcgcgtc ggcactcaca
     6721 aggtggcgag aggccggaat atagtctcgg tcagccttaa cccaacaggc aagctaatcg
     6781 ctgtgagtgt tagcgatatg cacagaacgg gcgagatccg cgattcagtg tacgtcatcc
     6841 gcagccgcga ccgggctgag gtcttccgcc gatatcttcc tccgctcact cagtcgaggg
     6901 tccagtttgt ggggccatcg cttcttgcct acactgattt tgacggttcg gacggatttg
     6961 ttcgagtggt caaggtgcct gagaacatcg acattgctaa tccgggatcg tagggccgat
     7021 ctcagacatt gtaagcatcc cgcgattagt ttccgaactt cccccgggcg actgaatctt
     7081 cgcgctcgaa cggaagggag aagccgctgc tcagtcccgc ttcccttcgt ggccgtactc
     7141 cgccgctact gcttgatgac cgtgccgtcg cggcggcgga cttcgcccca gcc
//
LOCUS       DATIFQ010000383         9981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_125410, whole genome shotgun sequence.
ACCESSION   DATIFQ010000383 DATIFQ010000000
VERSION     DATIFQ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125410"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..645)
                     /locus_tag="VLE22_18585"
     CDS             complement(25..645)
                     /locus_tag="VLE22_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSB16465.1"
                     /translation="MNSINFEAAFVNVALLWMLTVPHEFAHAWTAVRLGDDTPEREGR
                     VSLNPMAHVDWLGTVILPALTSLFGGGFLGWGKAVNTNPYRLRWGLNGLALVALAGPA
                     SNVVFALLLGLLTVLLHGTLPAVAEFTGRGVYLSLYLAMFNMIPVPPLDGSKLLLAAR
                     APVAVYNELARFGFVLLIVAISVSSLGRYLSLWSFQGTQVILRMFW"
     gene            769..3459
                     /locus_tag="VLE22_18590"
     CDS             769..3459
                     /locus_tag="VLE22_18590"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HSB16466.1"
                     /translation="MSHFVQTLVVCAVIAPAAAQQTPETQAPKPAEAKKPEPPKEKTF
                     AEIVKDAKEIKGLFTLWQTDEKVYLEVLPDQLDKIYMLSLTADSGIGERGFYAAAMTG
                     ETPILLRKRAKNLQFVAKNPRFRAAAGTPMSRFVERSFSDSTLGLAKIESLPHPERKS
                     VLIDLGSVLLTDLPMMSYALESTFRIPYRFDGKNSSFRTVKSYDRNVEIGVEAYYAVD
                     RPPVPPLRMPGAPAPPTPPRPRNLADIRGMFFLLRYSLSELPPPGYRARLADDRIGHF
                     FDQVEDFTSDVEHTPARRLVNRWRLEKEDPNASISRPKQPIVFWLENTIPVAYRPAIR
                     DGILMWNRAFERIGFRDAVVVKEQPDDAEWDAADVRYNTIRWFAGTDAGFAQGPSRTN
                     PYTGEIYDADIRFSESMVRFRRQTIVLQQNPLANPWDEQTAAYLRAPWGVEKGAMCTF
                     AAEAARDAEFAWDVLETRGIEPDSPEADKFVYSYLREIAAHEVGHTLGLRHNFRASTI
                     RRLDETQNASLTAKEGLTGSVMDYIPANLAGRGAKQGEYHQSTLGPYDYWAIEYAYKP
                     LAASTPEDEFPELRRIASRAAEPALAYATDEDAGLGSGAWDMDPDVSRFDLGSDPLAY
                     YVHRIRLSEEIQANLETALQKPGQGYQVLRRSFLSAVNMSGTALLLSAKYIGGVNHYR
                     DHVGDPGGRLPFRPVPAARQRQALNLIRDHLFAPDAFKLSPSLLNKLNNERFSDLRNF
                     ESMATRFDVPVHDVILMRQKAVLDRLYNPVVLKRILDSELSAPTDPFRLGDLFTGVQD
                     SVWAELKSPDAAVTINSFRRSLQREHLRRMIGMMLKDAAAPEDARTLARFTLASLKTR
                     VQGALARPGVKMTLETRAHLAESVSRIDEALKAGMERTAF"
     gene            complement(3481..6927)
                     /locus_tag="VLE22_18595"
     CDS             complement(3481..6927)
                     /locus_tag="VLE22_18595"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSB16467.1"
                     /translation="MEQDARAVSFEGLWDTELDEIAKSRTLRQERTAVAPPVPPVSGR
                     PSARAHDAALAGLAFSGGGIRSATFNLGVLQGLARQGILRRFDYLSTVSGGGYIGSWL
                     TAWIRREGIGAVERGLAPSPAETGSAQEPETIRVLRRYSNYLTPKLGVFSADTWTMIS
                     IYLRNLVLNQGILILALAAVMLLARPLAGFTRVMAENPEVSLMAAWALLGCALVSTGL
                     NLRALSRRPANGVYPRYCGPGCSLMSIVLPVLLASWAAAGGIWGLQASDVLIQRIDPA
                     AAPFHVTTFGTPAMVFLISFAATGIIGLWGPAMADEKREWVSRIGGWLMIACVAWVAF
                     FAMAFYSPLIFLTRESTMAELARRGFGPAWLATTAAGLLAGRSPSTGQRGSNRWLELL
                     AVVAPFVFIGGLLAAVALAIHQIHLSFVPEDVWSYWGQMHWPLDPGRRMQALGLALGA
                     AAVLTWAALFLSSRIDINQFSMHLFYRNRLVRCYLGASSHGRLPHPTTGFSAADDDML
                     IRRLAPSRIETCTDGTPHGYSGPYPIINAALNLTHGEELAWQQRKAASFVFTPLYCGF
                     DVPPARKDPCDFSADSPLAPAGYRPTGDYAYPGEGVSLGTALAISGAAASPNMGYHTS
                     SATAFLMTVFNVRLGWWLSNPRHNTTWKVRGPKLGLKYLFGELFGFTNDRSPYVYVSD
                     GGHFENLAVYELVRRRCRFIVACDGSEDRKYTFEGLGNAIEKCRADFGVPIEIDFEQL
                     RPADGQRLSQWHCAIGRIRYDLVDPQAPAGTLLYLKSSLTGEEPGDVKRYAAENPDFP
                     HQPTSDQWFDESQFESYRTLGEHVANTVFGAAEETRVLWQLSNEELFLALRQRWHPPA
                     VAAPGAFARHAAAVDRLIDMIRSSPELAFLDAQIFPEWQRLMSGNVTAPAPEMWLPRD
                     YVSRRAGFYACNQMIQLMETVYLDLNLEDSWDHPDNRGWMNLFQHWAWSGMFRVSWAI
                     GSSTFGARFQNFCRRRLSLDIRRGVLVGPPMPLEEAQRSDTLNFFEKELLEQFVEQNQ
                     PLGPDDVYPLHVMVDDPMRKTEDVAFLFTFGFALAGTVDGQKALLYFRIQNHLRKMGL
                     PREALAQLISNLDLQQTRLGLLQRSDDEAVTLEKRRQFAQLFETVKAEVTHGCKGPGA
                     AV"
     gene            7096..8304
                     /locus_tag="VLE22_18600"
     CDS             7096..8304
                     /locus_tag="VLE22_18600"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HSB16468.1"
                     /translation="MNHWLLLLCLVTAVSLAAQPRAVSDDEVMKVHRSALLIDTHNDV
                     PMRIVEGFDIGKASSKGHTDLPRLRQGGVGAVFFAAYVSAQYARSREAAHQALERIDA
                     IRHDIAGRYPNDFALSLTAADIEAARRQGKIAALIGIEGGHAIEDSLRVLRSFHALGA
                     RYVTLTHSNTNNWADSSGDIDNPKVKHHNGLATFGREVVREMNRLGMIVDVSHVSDKT
                     FWDVLETSQAPVFASHSSCRALASLHRNMTDEMIVAMAKKGGVIQINFACEFLSEESA
                     QAAKKLWPEYERLHAAARERFKDDSEGLKREVARLEADARRKTPRAKLTDVVAHIDHV
                     VKLAGIDAVGIGTDYDGITCAPEGLEDVSKFPNLTRALLEKGYSADNIRKIYGGNTLR
                     LMRAVERAAK"
     gene            8336..9562
                     /locus_tag="VLE22_18605"
     CDS             8336..9562
                     /locus_tag="VLE22_18605"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HSB16469.1"
                     /translation="MRSRREFVLGALAAPAVLKSAATPGYSWAEIEKIVARGDVRGKL
                     TRDDLPTPALLLDMDAFEANVAKMAGHLRERKRSFRPHAKTHKCPEIARRLVRAGAAG
                     VCAAKLSEAEVFAHDGVGGLLVTTAVIGRHKIERAVRLASRRPNTMFCVDNAQNVRDL
                     NDAAGAAKVRVNLAVDLWVGGRTGIVPGEPAVALAALVESLPHVKFAGLQSYAGHASH
                     TVGFENRTKVSHEAMGQAVETARLLAGKGIACPLVTGGSTGTYNIDSEIDGVTELQPG
                     SFIFMDLDYNRIGGADGPVYRDFKNSLTVLTTVVSRPNDSLAIVDGGLKAFSTDKPYM
                     PEAKGIQGIQYSWGGDEHGRLNIASASTAVNVGDRIEFIVPHCDPSVNLYDRIWCLRG
                     DQVEAVWPISARGMSQ"
     gene            complement(9637..>9981)
                     /locus_tag="VLE22_18610"
     CDS             complement(9637..>9981)
                     /locus_tag="VLE22_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16470.1"
                     /translation="PAGCQPGRYKLGEQEVDLTPQEKVVLAGTDRLAGSALRMDRGVE
                     NLMKLAGLSLRDAVTMATKNPARVGRIASRQRGLAPGDRGDIVQFRFDLERKAITVER
                     TWMAGEAVYAKG"
BASE COUNT         1928 a         3444 c         2889 g         1720 t
ORIGIN      
        1 gacgagtccg gcggtccgcc cgtcctacca gaacatccgg aggatcactt gggtaccctg
       61 gaagctccac agggagaggt accgccccaa cgacgacacc gagatcgcga cgatcagcag
      121 cacaaaaccg aaccgggcca gctcgttgta gaccgccacc ggcgcccgcg ccgccagcaa
      181 gagcttcgag ccatccagcg gaggcaccgg gatcatgttg aacatggcaa gatacaggct
      241 cagatagacg ccccggccgg tgaattccgc gaccgccggc aacgtgccgt ggaggagcac
      301 cgtcaagagc cccagcaaca gggcaaacac cacgttgctg gccggcccgg cgagcgccac
      361 caatgccagc ccgttcaatc cccaccgcag ccggtacggg ttggtattca ccgctttgcc
      421 ccaaccaagg aaccccccgc cgaacagcga agtgagcgcc ggcaggatga ccgttcccag
      481 ccagtcgacg tgggccatag gattgagcga gacccggccc tcgcgttcgg gtgtgtcgtc
      541 gcccagccgc accgccgtcc aggcgtgagc gaactcatga ggcactgtca gcatccacag
      601 cagcgcgacg ttcacaaatg ccgcttcgaa gttgattgaa ttcacgaaga tccttgagag
      661 gcgagttctg caacggaacg gccgtggggg actcaccgct gggcgcccct tccgtctatt
      721 gtatgatgtc gtcacacgcg ctaccggagg gaggaaagta tgcgatttat gtctcacttt
      781 gttcagacgc ttgtagtttg cgcggtcatt gctcccgcag ccgctcagca aacgcccgaa
      841 actcaggcgc ccaagccggc ggaagcgaag aaaccggaac cacccaagga aaagaccttc
      901 gctgaaatcg tgaaggacgc caaagagatc aagggcctct tcacgctctg gcagaccgac
      961 gaaaaagtct acctcgaagt gcttccagat cagcttgaca aaatctacat gctgtcgctc
     1021 accgccgact ccggtatcgg tgagcgcggc ttctacgccg cggccatgac gggtgaaacg
     1081 ccgattcttc ttcgcaagcg ggccaagaac ctccagttcg tagcaaagaa cccgcgcttc
     1141 cgggcagccg ccggcacacc catgagccgc tttgtggaac gatctttctc cgactcaacg
     1201 cttggccttg cgaagatcga aagccttccc cacccggaac gtaagagcgt cctcatcgac
     1261 ctcggatccg tgctgctgac cgaccttcca atgatgtcct acgcgctgga gtccaccttt
     1321 cgcattcctt accggttcga cgggaaaaac agctcgttcc gaacggtcaa aagctacgac
     1381 cgaaacgtcg agatcggcgt ggaggcgtac tatgcggtgg atcgcccgcc cgttccgcct
     1441 cttcggatgc caggcgcgcc ggcgccgccc acgcctccgc gtccacgcaa tctcgccgac
     1501 atccggggta tgttcttcct gcttcgctac agcctctccg aattgccacc acccggctac
     1561 cgggcgcgcc tcgccgacga ccggatcggg cacttcttcg accaggtcga agactttacc
     1621 tcggacgtcg agcacacgcc ggctcggcgc ctcgtcaatc gctggcgcct ggaaaaggag
     1681 gacccgaacg cgtccatctc ccgcccgaag cagccgatcg tgttctggct cgagaacacc
     1741 atcccggtag cgtatcgtcc cgccatccgc gacggcatcc ttatgtggaa tagagcgttc
     1801 gaacggatcg ggttccggga cgctgtcgtc gtcaaggaac aacccgatga cgccgaatgg
     1861 gacgccgccg acgtgcgcta caacacgatc cgctggttcg ctggcaccga cgctggtttc
     1921 gcacagggtc ccagccgcac aaatccgtac accggtgaaa tctacgacgc cgacattcgc
     1981 ttctccgaat cgatggtccg cttccggcgc cagacgatcg tgctccagca gaacccgctc
     2041 gccaatccat gggacgagca gacggcggcc tacctgcgcg ccccgtgggg cgtcgagaag
     2101 ggcgccatgt gtaccttcgc tgccgaggcg gcccgcgacg cagagttcgc atgggatgtt
     2161 cttgaaacgc gcggtatcga gccggacagt cccgaggcgg acaagttcgt ttacagctac
     2221 ctgcgtgaaa tcgccgcgca cgaggtcggc cacaccctcg ggctccgcca caatttccgg
     2281 gcgagtacaa tccgccgcct ggacgaaacc cagaacgcct cactcacggc gaaggaaggc
     2341 cttacaggct ctgtgatgga ttacatccca gccaacctcg ccggccgcgg cgcaaagcag
     2401 ggcgagtatc accagtccac gctcggtcca tacgattact gggcgattga atacgcctac
     2461 aagcccctcg ccgcctccac tcccgaggac gagtttcccg agttgcgcag gatcgcctcg
     2521 cgcgccgcgg agccggctct ggcatacgca accgacgagg acgccggcct cggcagtggc
     2581 gcgtgggaca tggaccccga tgtcagccgc ttcgacttgg gcagcgaccc gctcgcctac
     2641 tatgtgcacc gcatacggct gtccgaggag attcaggcca atctcgaaac cgcattacag
     2701 aaacccgggc agggctatca ggtcctccgc cgcagtttcc tgtcggcggt caacatgtcc
     2761 gggaccgccc tcttgctatc ggcgaaatac atcggcgggg tgaatcacta tcgcgatcac
     2821 gtcggcgacc cgggcggccg cctcccgttc cggcccgttc cagccgcgcg gcaaaggcag
     2881 gcgctgaatc tcatccgcga tcacctgttt gcgccggacg catttaagct gtccccatcg
     2941 ctcctcaaca agctcaacaa cgagcgcttc tccgacctcc ggaacttcga gtcgatggca
     3001 actcgattcg acgtgccggt ccacgacgta atcctgatgc gccagaaagc ggttcttgac
     3061 cgcctgtaca atcctgtcgt gctgaagcgc atcctcgatt cggaactcag cgctcccacg
     3121 gatccattcc gcctcggcga cctcttcacc ggagtccagg actcggtgtg ggccgaattg
     3181 aaaagcccgg atgctgccgt gaccatcaac agcttccgcc ggtcgctcca gcgtgagcac
     3241 ctgcggcgca tgatcggcat gatgctcaaa gatgccgccg cgcccgagga cgcccggacg
     3301 ctggctcgct ttactcttgc ctcgctcaag acccgagttc agggcgcttt ggccaggccc
     3361 ggcgtgaaga tgacgctcga aacgcgcgca cacctcgcgg aatcggtctc tcgaatcgac
     3421 gaggccctca aggccggaat ggagcgcacc gcattctgac agccgccccg gcaggcggcg
     3481 tcataccgcg gcgcccggac ccttgcagcc gtgtgtgacc tcggccttca cggtttcgaa
     3541 cagttgcgcg aactgccgcc gcttctcgag tgtgaccgct tcgtcgtcac tgcgctggag
     3601 caggcccaga cgcgtttgct ggaggtccag attgctgatt agctgcgcca gcgcctcgcg
     3661 cggcagcccc atctttctca ggtggttctg aatgcggaag tacagtaaag ccttttgtcc
     3721 atccaccgtt cccgccagtg cgaaaccgaa tgtgaagagg aacgccacgt cctccgtttt
     3781 gcgcatcgga tcgtctacca ttacgtggag cgggtagaca tcgtccgggc cgagcggctg
     3841 gttctgctcg acgaactgct ccagcagctc cttctcaaag aagttcagcg tgtccgaccg
     3901 ctgtgcctcc tcgagcggca tcggaggccc caccagcacg ccccggcgaa tgtccagtga
     3961 cagccgcctc cggcagaagt tctgaaatct agcgccaaat gttgagcttc cgatcgccca
     4021 cgacacccgg aacatccccg accacgccca gtgctggaac aggttcatcc agccgcggtt
     4081 gtccggatgg tcccacgagt cttcgaggtt caaatcgaga tagacggtct ccatgagctg
     4141 gatcatctga ttgcaggcgt agaaccccgc gcgcctcgaa acgtagtcgc ggggaagcca
     4201 catctccggc gcgggcgccg tcacgttccc gctcatgagc cgctgccact cggggaagat
     4261 ctgcgcatcg agaaaagcca gctccggact tgaccggatc atatcgatga gccgatcgac
     4321 cgcggcagcg tgccgcgcga atgctccagg cgccgccacg gcgggtggat gccaccgctg
     4381 ccgcagcgcc agaaagagtt cctcgttcga caactgccat agcaccctgg tctcttcggc
     4441 cgctccgaac accgtgttcg ccacgtgctc gccgagcgtg cggtagctct cgaattgcga
     4501 ctcgtcaaac cactgatccg aagtcggctg gtgcggaaaa tccggattct cggccgcata
     4561 ccgctttacg tcgcccggct cctctccggt gagagacgac ttaaggtaca gaagcgtgcc
     4621 tgccggagcc tgggggtcca ccaggtcgta ccggattctg ccaatcgcgc aatgccactg
     4681 gctcaggcgc tgcccgtcgg ccgggcgcag ttgctcgaaa tcgatctcga tcggcacgcc
     4741 gaaatcggcc cggcatttct cgatggcgtt gcccagcccc tcgaacgtgt atttccggtc
     4801 ttcgctgcca tcgcatgcta cgatgaaccg gcaccggcgc cgcaccagtt cgtacactgc
     4861 caggttttcg aagtggcccc cgtcggacac gtaaacgtat gggctgcggt cgttcgtgaa
     4921 gccaaacagc tcgccgaaca ggtacttcag accgagtttc ggcccacgca ccttccatgt
     4981 ggtgttgtgg cgagggttgc tcagccacca cccaagccgc acgttaaata cggtcatcag
     5041 aaaagcggtt gccgatgacg tgtggtatcc catgttcgga cttgcggccg cgcctgaaat
     5101 cgctagcgcc gttcccaggc tgaccccttc gccggggtac gcatagtccc cagtggggcg
     5161 gtaaccggcc ggagcgagcg gggaatcggc actgaaatcg cagggatctt tgcgtgccgg
     5221 cggcacgtcg aagccgcaat acagcggcgt gaagacgaac gaggctgcct tgcgttgctg
     5281 ccaagccaac tcctcgccgt gtgtcaggtt gagcgccgcg ttgatgatcg ggtacggtcc
     5341 gctgtagccg tgcggtgtgc catccgtgca ggtttcaatc cgcgacggcg ctaaccggcg
     5401 gatcagcata tcgtcgtcgg cggcgctgaa gccggtggtc ggatgaggca gccgcccgtg
     5461 gctggacgcg ccgagatagc atcgcaccag ccggttccgg taaaacaggt gcatcgaaaa
     5521 ctgattgatg tcgatgcggg acgagagaaa tagtgccgcc caagtcagca cggcggccgc
     5581 acccagcgcc agccccaacg cctgcatcct ccggcccgga tcgagcggcc agtgcatttg
     5641 tccccagtag gaccacacat cctccggtac gaacgacagg tgaatctggt gaatcgcaag
     5701 agccaccgcc gccaacaaac ccccaatgaa cacgaacgga gcaaccaccg ccagcaattc
     5761 aagccaccgg ttcgatccac gctgcccggt cgaggggctg cggccggcta gcaggccagc
     5821 cgcggtggtt gccagccacg cagggccgaa tccgcgccgc gcaagctcgg ccatagtgct
     5881 ttcgcgggta aggaagatca gcgggctgta gaatgccatc gcaaagaagg ctacccacgc
     5941 cacgcacgca atcatcagcc acccgccgat ccggctcacc cactctcgct tctcgtccgc
     6001 cattgccgga ccccacagcc cgatgatccc cgtcgcagcg aaggaaatca ggaacaccat
     6061 cgccggcgtg ccgaacgtgg tcacatgaaa cggtgctgcg gccggatcga tccgctgaat
     6121 caacacgtcg ctggcttgga gtccccagat tccccccgcc gctgcccacg acgctagcag
     6181 caccgggagg acgatgctca tcagtgaaca tcccgggccg caatagcgtg gatagactcc
     6241 gttagccggc cttcgggaca gcgctctaag attgaggccg gtcgagacca gcgcgcaccc
     6301 gagcagggcc caggccgcca tcagagaaac ctccggattt tccgccatca ccctggtgaa
     6361 accggccagc ggccgtgcca gtagcatcac cgccgccagc gcaagaatca ggattccctg
     6421 gttcagcaca aggttccgga ggtagatgga gatcattgtc caggtgtccg cgctgaacac
     6481 gccaagtttc ggcgtcaggt agttgctgta acggcgcagc acgcggatcg tctcgggctc
     6541 ttgcgccgaa ccggtctcgg cggggcttgg ggcaagcccc cgctccactg cgccgattcc
     6601 ctcgcggcgg atccacgccg taagccagct cccgatgtag cctcctcccg agactgtcga
     6661 aagatagtcg aatctgcgca gaatgccctg ccgggccagc ccttgcagca cgccgagatt
     6721 gaaggtcgcg ctccggatgc caccgcccga aaacgccagc cccgccagcg ccgcgtcgtg
     6781 agccctcgcc gagggcctcc cgctgaccgg cggtaccggc ggcgcgacgg cggtcctctc
     6841 ctgccgtagc gtcctcgact tcgcaatctc gtccagctcc gtatcccaca gcccctcgaa
     6901 cgatacagca cgcgcatcct gttccacagt gagccctcca gcctcaatat ctagtgagaa
     6961 tactccagcc gcgtgttaca tggtcggcac ttattcttca catgagcaga ctgaagcgat
     7021 atggagccgc gagcgtcagc gagcggcgct gtcttcttcc cgcctgtgcc acaatcagac
     7081 tttccggagc accacatgaa tcattggctg ttgctgttgt gcctcgtgac cgccgtttct
     7141 ttggccgcac agccgcgcgc ggtctccgac gacgaggtca tgaaggtcca ccgctcggcg
     7201 ctgctcatcg atacccacaa cgacgtgccg atgcgcatcg tcgagggttt cgacatcggc
     7261 aaagcatcct ccaagggcca caccgacctc ccgcgcctgc gccagggtgg cgtcggcgca
     7321 gtcttctttg ccgcgtatgt ctccgcccag tacgcccgct cccgagaagc cgctcatcag
     7381 gccctcgaac gaatcgacgc catccgtcac gacatcgccg gccgctatcc gaacgatttc
     7441 gcactctctc tgaccgccgc cgacatcgaa gccgcccgcc ggcagggcaa gatcgccgca
     7501 ctgatcggca tcgaaggcgg ccacgcgatc gaggacagcc tccgcgtgct ccgctcgttc
     7561 cacgctctcg gcgcccgcta cgtgacgctt acccacagca acacgaacaa ctgggccgac
     7621 tcctccggcg atatcgacaa tcccaaggta aagcaccaca atggcctcgc gaccttcgga
     7681 agggaagtcg tccgcgagat gaatcggctc ggaatgattg tcgacgtgtc ccacgtctcc
     7741 gacaagacct tctgggatgt tcttgaaact agccaggcgc cggtgttcgc gtcacactcc
     7801 tcctgccgcg cgctcgcctc gctccaccgc aacatgaccg acgagatgat cgtcgccatg
     7861 gcgaagaagg gcggcgtcat ccagatcaac ttcgcctgcg agttcctctc cgaagaatcc
     7921 gcgcaagccg cgaagaagct ttggcccgaa tacgaacggc ttcacgcggc cgcccgcgag
     7981 cggttcaagg acgattctga agggctgaaa cgcgaagtcg cccgcctcga agccgacgcc
     8041 cgtcgcaaga caccccgcgc aaaactgact gacgtcgtgg cgcacatcga tcacgtcgtg
     8101 aagctcgccg gcatcgacgc cgtcggcatc ggcaccgact acgacggcat cacctgcgcg
     8161 cctgaaggcc tcgaggacgt ttcgaagttt cccaacctca cgcgcgctct gcttgagaag
     8221 ggatactccg cggacaacat ccgtaagatt tatggtggca acacgctccg cctgatgagg
     8281 gcagtggaac gcgcggcaaa gtaggactac actgggtgga catctggagt cccacatgag
     8341 atcgagacgc gaatttgtcc tcggtgccct tgccgcaccc gccgttctga aatccgccgc
     8401 cacgcccggc tactcgtggg ccgaaatcga gaagatcgtc gcccgcggcg acgtgcgcgg
     8461 caaactcacg cgcgatgact tacccacccc ggcgctcctg ctcgacatgg acgccttcga
     8521 ggccaacgtc gcgaaaatgg ctggccatct tcgagagcga aaacgctcct tccgtccgca
     8581 cgccaagact cacaagtgcc ccgagatcgc ccgtcgtctc gtccgggcgg gcgccgctgg
     8641 cgtttgcgcg gcgaaactga gtgaagcgga ggtcttcgcg cacgacggcg ttggagggct
     8701 gctcgtgacc accgccgtca tcggccgcca caagattgag cgtgccgttc gtcttgcatc
     8761 ccgccgcccc aacacgatgt tctgcgtcga caacgcccag aacgtccgcg acctcaacga
     8821 cgccgccggc gccgcgaagg tccgggtcaa cctcgcggtc gacctgtggg tcggtggccg
     8881 taccggaatc gttcccggcg aaccggccgt cgcgcttgcc gctctggtcg aatcgctgcc
     8941 gcacgtgaag ttcgcaggcc ttcagtcgta tgccggccac gcctcgcaca cggtggggtt
     9001 tgagaatcgg acgaaggttt cccacgaggc aatgggccag gcagtcgaga ccgcgcgcct
     9061 gctcgcgggg aagggaattg cctgccctct cgtgacgggc ggctccaccg gtacctacaa
     9121 catcgactct gagatcgacg gcgttaccga gctccagccc ggcagcttca tcttcatgga
     9181 tctcgattac aaccgcatcg gcggcgccga tggacctgtc taccgcgact tcaagaactc
     9241 gctcactgtg ctgactaccg tcgttagccg gccgaacgac tccctcgcca tcgtcgatgg
     9301 cggcctcaaa gcgttctcga ccgacaagcc ctatatgccc gaggcgaagg gcatccaggg
     9361 gattcaatat tcctggggag gcgacgaaca cggacggctc aacatcgcga gcgcctcaac
     9421 cgccgtcaac gtcggcgacc gcatcgagtt catcgtgccg cactgcgacc cttcggtgaa
     9481 cctctacgat cgcatctggt gcctgcgggg cgaccaggtc gaggccgtct ggcccatctc
     9541 cgcccgcggc atgagccagt gatgtgctga gcgggacagg cctaccctgt cctgagcgca
     9601 gtttgcgagc ggacagagct gcctttcccg gactgcctac cccttcgcgt acaccgcctc
     9661 gcccgccatc cacgtccgct ccacggtgat cgccttccgc tccaggtcga aacggaactg
     9721 cacgatgtct ccgcggtcgc cgggcgccaa gccacgctgt cgtgaagcga tccgtcctac
     9781 ccgagcaggg ttcttcgtcg ccatcgtcac cgcgtcgcgc aacgacagtc ccgccagctt
     9841 catcagattc tcgacgcccc ggtccatccg tagcgccgac cctgccaagc ggtctgttcc
     9901 cgccaacacg accttctcct gtggcgtcag gtcgacttcc tgctcgccga gcttatatct
     9961 cccaggctgg cagcccgccg g
//
LOCUS       DATIFQ010000384         3038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1851399, whole genome shotgun sequence.
ACCESSION   DATIFQ010000384 DATIFQ010000000
VERSION     DATIFQ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851399"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VLE22_18615"
     CDS             <1..313
                     /locus_tag="VLE22_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16471.1"
                     /translation="MDSGLPNTIGAGEPVATQEDRADLFAAYAQDLARLPVCVGYFWF
                     KYRDQPKEGQGGRSPGGWGGENSNYGLVRLDGTAWTVLTNRMTAVNAGIEELAASTHD
                     R"
     gene            471..806
                     /locus_tag="VLE22_18620"
     CDS             471..806
                     /locus_tag="VLE22_18620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSB16472.1"
                     /translation="MKEIAIREFLETSGRSPFGRWFEGLNAVAAARVTIALARLGQGN
                     FSNVEGVGAGVFEIKIDFGPGYRVYFGKDDERIVVLLGGSAKKRQSAAIAAAQAAWVE
                     YKRRKGGME"
     gene            812..1126
                     /locus_tag="VLE22_18625"
     CDS             812..1126
                     /locus_tag="VLE22_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16473.1"
                     /translation="MALTRAFRETVYQRAQRDSAFRKALLMEAVNAYLGGDEATGKVV
                     LRDVINATVGFEPLAAELGKPAKSLHRMLGPGGNPSTANFFAILRVLQKRAGVKLTVK
                     AA"
     gene            1200..1928
                     /locus_tag="VLE22_18630"
     CDS             1200..1928
                     /locus_tag="VLE22_18630"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB16474.1"
                     /translation="MPRRARVVFPGLPHHVTQRGNNRQQLFFAPEDYRYYLELLADQA
                     RRCGVEVLGYCPMPNHVHLIAAPENEASLGRALGRTHSEFALAVNRTEGRTGHVFQNR
                     FFSCVLGGTHLLRALRYVERNPVRAGLAAVAWGWPWSSASVHTAANAVDAVISFDWIG
                     QFGRWDYGEWKELLSEGGAGDVEEAIRRATRTGEPVGSRDFVAGLERRAGKRLVVLER
                     GRPRRVPQSPEERARQGCLFQGIE"
     gene            2073..2549
                     /locus_tag="VLE22_18635"
     CDS             2073..2549
                     /locus_tag="VLE22_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16475.1"
                     /translation="MIKLLSAILGVFFIPNAGVAADPCSARQGALVELKALKANPQKF
                     NGKTIRVSGMLRSGHVAVSLQNGEGLSVRIRARDEVKSRHLHRACEDTLYQRLWALSE
                     SPRTPEDEVLYLVDAEGVVRTLKDRSGKPAKEFSVFGQWPVELIVTRVLSIHENGR"
BASE COUNT          594 a          809 c         1079 g          556 t
ORIGIN      
        1 gatggacagc ggtcttccga ataccatcgg cgcgggcgaa ccggtggcca cccaggaaga
       61 ccgcgcggac ttgttcgccg cgtatgcgca ggacctggcg cgcctgccgg tttgcgtcgg
      121 gtatttctgg ttcaaatacc gtgaccagcc caaggagggg caaggcggcc gcagccccgg
      181 cggatggggc ggcgagaaca gcaactacgg tctcgtgcgc ctggatggaa ccgcctggac
      241 tgtgctgacc aatcgaatga cggcggtcaa tgcgggcatc gaggagctag cagcgagcac
      301 acacgaccgc tgatcctcag gcatgacgaa tagccgtcac ggccaccggc attgggaccc
      361 tttcggcacg ctgggcacag cgggagctcc gaaaaacagg ttgataggtt gatacgctgc
      421 ggccgtcatg gcggtgcgcg gatggtaacg tatgtgttac cataaacgcg gtgaaggaaa
      481 tcgcgatccg ggagttcctg gaaacctcgg gccggtcgcc gtttgggcgc tggtttgagg
      541 gcttgaacgc ggtggcggcg gcgcgggtga cgattgcgct cgcacggctg ggccagggga
      601 acttctcgaa tgtcgaaggc gtaggtgccg gagtgttcga gatcaagatc gatttcggcc
      661 cggggtatcg ggtctacttc ggcaaggacg acgaacggat cgtggttctg ctgggaggga
      721 gcgcgaagaa acggcagagc gccgcgattg cggccgcgca ggcggcatgg gtcgagtaca
      781 agcgccgcaa gggcggcatg gagtagggca aatggcattg acaagagcgt ttcgggagac
      841 ggtttaccag cgggcgcagc gggacagcgc gttccgcaag gcgctgctga tggaggcggt
      901 gaacgcttat ctcggcgggg atgaggcgac ggggaaagtg gtgttgcggg atgttatcaa
      961 cgcgactgtt ggcttcgagc cgttggccgc ggagttgggg aagccggcca aaagcctgca
     1021 ccgcatgctg ggccccggcg gcaacccgag tacggccaac ttcttcgcga ttctccgagt
     1081 gctgcagaag agagccggcg tgaagttgac cgtgaaggcg gcgtaggatc ggctaaaggg
     1141 gctaaagggg acagacgcat tttcctgaga gaaggcgccg cgttcgcaca cggatgtatg
     1201 tgccccgccg cgctcgcgtc gtttttcccg gcctgccgca tcacgtcacc cagcgaggga
     1261 acaaccggca gcagcttttc tttgcgccgg aggattaccg gtattatctg gaactcctgg
     1321 ccgaccaggc gcggcggtgt ggggttgaag tgctcggtta ctgcccgatg ccgaatcatg
     1381 ttcacttgat cgccgcgccg gaaaacgagg cgtcgcttgg ccgtgcgttg ggacggacgc
     1441 actcggagtt cgccctggct gtgaaccgga cggaggggcg taccggccac gtgtttcaga
     1501 accgtttctt ctcgtgcgtt ttgggtggga cccacctgct gcgcgcgctg cgttacgtgg
     1561 aacggaaccc ggtccgagcc ggactggcgg cggtggcctg ggggtggccc tggtcgagcg
     1621 ccagcgtgca caccgcggcg aacgcggtgg atgcggtgat aagcttcgat tggatcggcc
     1681 agttcggccg gtgggactat ggcgagtgga aggaattgct ctcggagggc ggggccggcg
     1741 acgtggagga agccatccgc cgggcgacgc ggaccgggga gcccgtgggg tctcgcgatt
     1801 tcgttgccgg gttggagcgc cgggcgggaa agcgactggt ggttctcgaa cgcggccggc
     1861 ccaggcgggt gccgcaatcg ccagaggaac gtgcccgtca gggctgcctc tttcagggaa
     1921 tcgagtgagc caaagtataa tgcgtctgtc ccctttctcc tttggaaccc gagttgggat
     1981 gcagctgacc aggagacgat gcgggccagg gccgtgaaag aaggcgccca aagatccgta
     2041 caagcggtta aggacgagct ggctgggcga aaatgataaa gctgctgagt gcgattttgg
     2101 gcgttttctt catcccgaac gccggagtcg cggctgaccc atgttcggcc cgacagggcg
     2161 ctctcgtcga actcaaggcg ctgaaggcca atcctcagaa gtttaatggg aagaccatcc
     2221 gcgtttcagg catgctgaga tccggccatg tagccgtctc gttgcagaat ggcgaaggtc
     2281 tttcagttag aatccgggca cgcgacgaag tgaaaagtcg ccatctgcat agggcatgtg
     2341 aggatacgct ctaccagcgc ttatgggctt tgagtgaatc gccaaggact cctgaggatg
     2401 aggttttata tctagtcgat gcggagggcg ttgtgaggac gctgaaggac cggagtggaa
     2461 agccggcaaa ggagttctcg gtgtttggac agtggccggt ggagttgatc gttactcggg
     2521 tgctcagcat acacgagaat ggacggtgaa gttcaccggc aaagaacggg atgcggagac
     2581 cagcctcgat tatttcggag ccaggtactt ctctggcgcg cagggaaggt tcacgagccc
     2641 ggacgcgctg atgggctcag cacaactttc gaatccacag acttgtaacc gatactcgta
     2701 cgggctgaat aacccgctca gaaacactga cccgtcaggg ctttacaatt gggactgctc
     2761 tgctggatgc agctactcgg acgacgagtt gagaaagcga gcagccgaca aagaccgcag
     2821 caaggcggag cgcggctaaa ggggacagac gcattttcct gagagaaggc gccgcgttcg
     2881 cacacggatg tatgtgccgc gccgcgctcg cgtcgtcttt cccggcctgc cgcatcacgt
     2941 cacccagcga gggaacaacc ggcagcagct tttctttgcg ccgggaggat taccggtatt
     3001 atctggaact cctggccgat caggcgcggc ggtgtggg
//
LOCUS       DATIFQ010000385         6141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2619665, whole genome shotgun sequence.
ACCESSION   DATIFQ010000385 DATIFQ010000000
VERSION     DATIFQ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619665"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VLE22_18640"
     CDS             <1..675
                     /locus_tag="VLE22_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16476.1"
                     /translation="NEELMKRVAVFSLHSYGQQSITPQVDRVRNSKSPQLPVWLTEYG
                     DLNDLDKSKDNEWKNFSLAATKRALRALNEGASVALFWDAYDNYHEHYPRLTFYGLVQ
                     NTDHIYAPKKRYFAAKQLYHFVRPGSQRIALNSDATGLLLSAFRNAADGTLTVVGVKE
                     GAPTEFDLVLTGAGARIGAWDVYVTTRDVDCRKMDTVAIKDGTARISLPPEAVFTLVT
                     TMKEAE"
     gene            719..1744
                     /locus_tag="VLE22_18645"
     CDS             719..1744
                     /locus_tag="VLE22_18645"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB16477.1"
                     /translation="MKTLGAGIIGTGWVSEEYIKAFQKNPHCEVVALCSRDAARAKAK
                     AAEHRLQNCAAFANPADMLKASGLDIVVVCTPHHLHVEQGVAAARAGKHVIVEKPIAI
                     SVPSLRQLDKEIRSASVKSIVSFVLRWNPMFDNIRAILDQGLIGKVFYAEVDYLHGVG
                     PWYQLWSWMAKKEYGGSSLLAGGSHAVDGLRWFVRDEAVEVTAYSNTSRQNPLKYEYD
                     PNIVTIIRFAGGAIGKVACSLECRMPYVFNVELFGDKGTIRNNQLFTTEWPGQNSWAA
                     IPAVLPDSGDVSHHPFQQEVDHFVECILNDKESHANVADTYKTHEICLAADISAAEGR
                     PVRLPLP"
     gene            complement(1770..2726)
                     /locus_tag="VLE22_18650"
     CDS             complement(1770..2726)
                     /locus_tag="VLE22_18650"
                     /inference="COORDINATES: protein motif:HMM:NF015132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSB16478.1"
                     /translation="MSATKSWIYLLLCNLMWSLQFTCIKLVQDQVGPFATVWGPMTLA
                     TLMLYPLVRREAKSHPQKGERGRKDILLFMTLALLGVFPGQFFVTWGTQLSLASNAAL
                     LMLTLPICTAFMAYLFLGEKMTGIRWVSFAFALVGVVMCSEIDFGTLNFGATYLAGNA
                     LIFAGMLGCAYYNSYGKKVLQRYSPLELLFYTYVAMFVIMTPLTLATEADVFARIPNF
                     TLQTWIGLLLLIFFHNYLSMVLFLKALDYLDAIQAALSNYLITFFGLPISAIWLGERL
                     TPMMIAGGVIVLLSTLLITVWEERKRRSVPAVEAADPRPIRD"
     gene            complement(2723..3682)
                     /locus_tag="VLE22_18655"
     CDS             complement(2723..3682)
                     /locus_tag="VLE22_18655"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HSB16479.1"
                     /translation="MPEPQSFRIDGIVPIIPTPFTREQEIAWDELPGLIEFANVAGAS
                     AVCLPAFASEFYKLSETERMQLVREAVKHSAGRIPVIAQVNYPSAIAAAEMACRGQDL
                     GATAIAVTVPRLFALGEKDLFRYFERILQAIRLPLVIQDFNPGGPTVSPGFVKTLHQA
                     YPHFRYIKLEEPMMAAKTKRIIEESDGEVGVLEGWGGMYILELIDAGISGVMPGLGLT
                     DLLARVYRLASDGKKEEAYGIFTGVLPQIVFSLQNLELFHRAEKLLLQARGLLTETHV
                     REASLEIDPGDTEHIRFLNRRILSLLDSLGMPANPVSHIGRAK"
     gene            complement(3702..5756)
                     /locus_tag="VLE22_18660"
     CDS             complement(3702..5756)
                     /locus_tag="VLE22_18660"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HSB16480.1"
                     /translation="MRAIIGLLVVLLPAAAASHNPLLPRPQEVRYGPGKLSLRGLTIR
                     LASTPGAEDKFAARELSSALSAAAQSSVSVTEGSGSGRAIVLAQTGNAPALPMRDERG
                     GPDSREAYSLKITAEGAEIRAKSSAGLFYGVQTFRQLVEGSGSQAALPEVEIRDWPSL
                     AYRGFMMDTNHGALLTEDEIKRQLDFLARWKANQYYFYAEANIEVRGYPLVNLESRYS
                     QQQIKRIIGYARQRHIDVVPCMEFYGHMHDVFRVEQYSDMAPLRYGGEFNPRHPRVRA
                     FLDDWVGQMAQLFPSPWFHIGFDEPWALELFGSPVAGAPPAELFMEHLKNVSAIVLNH
                     NKRMMFWADMIAGARIFSRYPELISQLPKETIAVPWHYRVEKDFTPIVEPLAKAGLPM
                     VVAPGVWNWNEVAPSFQTSFGNISGMLAAGRKYGAMGLLNTGWSDDAQTIYRMSLPGM
                     AFGAAAPWQSSPVDQEQFFSDYARQMYLPSVATEVAQGLAALTKSQETFFTALGGPTM
                     LHFWDDPFEPDRLKQLEQREKELREARLSAEEATTHLLEALRLKGDPATLSSLLLGAR
                     MLDHLGMKNLYAIEIAKYFRMLGPDAPRDKVNLLLRTQTGSHNHGLLQDLMDSTMELK
                     ALYRQAWLSEYQDYRLATALGKWDAEFEYWWKMQKKVQDVIGGLKPGEPVPPLDSFRT
                     AR"
     gene            complement(5782..>6141)
                     /locus_tag="VLE22_18665"
     CDS             complement(5782..>6141)
                     /locus_tag="VLE22_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16481.1"
                     /translation="IAFHDRDVAMDKGAIERLLNDLGKGVRYMTAGEYCAYLHSTVAR
                     DPSPSESLALSLMYDDHYCRHFDSHESTWTLHLSDELRSSLKSQQPEKQTVVVPRGLG
                     RHILNVSAEGVLLRRGS"
BASE COUNT         1315 a         1787 c         1829 g         1210 t
ORIGIN      
        1 aacgaggaac tgatgaagcg ggtcgccgtc ttttccttac acagctacgg ccagcagtcc
       61 atcacccctc aggtggaccg tgtccggaac agcaagagcc cgcagctccc ggtctggctg
      121 acggaatatg gagacctgaa cgacctcgac aagtccaagg acaacgaatg gaagaacttc
      181 tcacttgctg ccaccaagcg ggcgttgcgc gccctcaacg agggcgcaag cgtagcgctg
      241 ttctgggacg cttacgacaa ctaccatgag cactatccgc ggctgacgtt ctatggactc
      301 gtgcagaaca cggatcacat ttatgcgccg aagaaacgct acttcgccgc aaagcagctc
      361 taccattttg tccgtccggg ttcgcagagg atcgcactga acagcgacgc aacggggttg
      421 ttgctatccg catttcgtaa cgccgcggat ggtacgctga cggtggtcgg cgtcaaagag
      481 ggcgccccca ccgagttcga tcttgtcctg accggggccg gcgcgagaat tggagcgtgg
      541 gacgtttacg tgacaaccag ggatgtggat tgcaggaaga tggacacagt cgccatcaag
      601 gacggaacgg ccaggatcag cttaccgccg gaagccgtct tcacgctcgt cacaacgatg
      661 aaagaagctg agtgacaccg gcgaaggtaa cctacaatcc cagaaggagc tgatacggat
      721 gaaaacactt ggtgcgggca ttattggaac cggatgggtt tcggaagagt acatcaaagc
      781 gtttcagaag aatcctcact gtgaggtggt cgccctatgc agccgggacg ctgcgcgggc
      841 gaaggcgaag gccgccgagc atcgccttca gaactgtgct gccttcgcta atccggcgga
      901 catgttgaag gcgtcaggcc tcgacattgt tgtcgtctgt acaccccatc atctgcacgt
      961 cgagcaaggt gttgccgctg cccgcgccgg caagcacgtc atcgtcgaga aaccgattgc
     1021 gatcagcgtg ccgagcctac gccagctcga taaggagatc cggtcggcca gcgtgaagag
     1081 tatcgtcagt ttcgtcctgc gttggaatcc gatgttcgac aacattcggg cgatcctgga
     1141 ccagggactg atcggcaagg tcttctacgc tgaggtcgat tatctgcacg gagtcggacc
     1201 ttggtaccaa ttgtggtcct ggatggccaa gaaggaatac ggcggcagtt ctctgctggc
     1261 aggcggcagc cacgcggttg acggactccg ctggttcgtg cgcgacgagg ccgtcgaggt
     1321 taccgcatac tcgaacacga gcaggcagaa cccgctgaag tacgagtacg acccgaacat
     1381 cgtcacgatc atccggttcg caggcggcgc gatcggcaag gttgcctgct cgctggaatg
     1441 ccggatgccc tatgtgttca acgtggagtt gttcggcgac aagggcacaa tccgcaacaa
     1501 ccagttgttc accacagagt ggccgggcca gaactcctgg gcagcgattc cggcggtcct
     1561 ccccgatagc ggcgatgtct cccatcatcc gttccagcag gaagtggatc atttcgttga
     1621 gtgcattctg aacgacaagg aatcgcacgc gaacgtggcc gatacgtaca agacccacga
     1681 aatctgcctg gcggcggata tttcggcggc ggagggcaga cccgtccgcc tgccgttgcc
     1741 gtaagtcagc cgttcgtagc gattgaactt caatcccgga ttggtcgagg gtcggcggcc
     1801 tcaacggcgg ggactgagcg gcgtttcctc tcttcccaca ctgtgatcag cagtgtgctg
     1861 agcaggacga tgacgccgcc cgcgatcatc atcggagtaa gacgctcgcc gagccaaatt
     1921 gccgaaatcg gcaggccgaa gaacgtaatc agatagttgg acagcgcggc ctgaatcgcg
     1981 tccaggtagt ccagtgcttt caggaagagg accatcgaaa gatagttatg gaagaaaatc
     2041 agcagcagga ggccgatcca ggtttgcagg gtgaagttgg ggatgcgagc gaatacatcc
     2101 gcttcggtcg ccagagtgag aggcgtcatg atgacaaaca ttgcaacata agtgtagaag
     2161 agcagttcca gcggagaata acgctgcagg accttcttgc cgtaggagtt gtagtaagcg
     2221 caaccgagca tccccgcgaa aatgagagca tttcccgcca ggtacgtggc gccgaagttg
     2281 agcgtgccga agtcgatttc cgaacacatg acgacaccca ccagggcgaa agcaaagctg
     2341 acccagcgaa taccggtcat cttctccccc agaaagaggt acgccatgaa cgccgtacag
     2401 atcggcagtg tcagcatcag cagcgctgca ttgcttgcca gagacaattg cgtgccccac
     2461 gtgacgaaaa actgcccggg aaaaacgccg agcagagcca gtgtcatgaa cagaaggatg
     2521 tccttgcggc ctctttcacc cttttgtggg tgactcttcg cctcgcggcg tacgagcgga
     2581 taaagcatga gagtcgccag cgtcatggga ccccaaactg tggcaaacgg tcccacctgg
     2641 tcctgcacca gtttgatgca ggtgaattgc agtgaccaca tcagattgca gagcaacagg
     2701 tagatccagg atttggttgc gctcattttg cccttccgat atggctcacg ggattcgccg
     2761 gcattcccag gctgtccagc agcgagagga tgcgcctatt caggaaccgg atgtgttcgg
     2821 tgtctcctgg atcgatttcc agactcgcct ctctaacgtg cgtctcggtg aggaggccgc
     2881 gcgcctgaag cagaagtttt tccgcacggt ggaacagttc cagattctga agactgaaaa
     2941 cgatctgcgg cagaacaccg gtaaatattc cgtatgcttc ttctttctta ccgtctgagg
     3001 cgagccggta gacccgcgcg agcagatcgg tgaggccgag tcccggcatc acgccggaga
     3061 tccccgcgtc gatcaactcc aggatgtaca tgccacccca accttcaagt acccccacct
     3121 ccccgtccga ctcttcgatg attcgcttcg tcttggcggc catcatcggc tcttccagct
     3181 tgatgtagcg aaagtgaggg tacgcctgat gcagcgtttt aacgaagccc gggctcactg
     3241 tcggcccgcc cggattgaag tcttgaatca cgagcgggag gcggattgcc tggagaattc
     3301 gctcgaagta gcgaaacagg tctttctcgc cgagcgcaaa caggcgcggc acggtgaccg
     3361 cgatggctgt ggctccgagg tcctgtccgc ggcacgccat ctccgccgcc gcgatcgccg
     3421 acgggtagtt cacctgggca atcaccggaa tcctcccggc cgagtgcttg acggcctccc
     3481 gaaccagttg catacgttct gtttcggaaa gtttgtagaa ctcgctcgca aaggccggca
     3541 ggcagacggc agaagcgccc gcaacgtttg cgaactcaat caggcctggc agttcatccc
     3601 acgcgatctc ctgttctcgt gtaaatggag tggggataat cgggacaata ccgtcgatcc
     3661 gaaacgattg gggctctggc ataaatgttc ctcagctcac tttaacgagc ggttcggaaa
     3721 gaatcgaggg gcggcacagg ttctccgggc ttcaagccgc caatgacgtc ttgcactttc
     3781 ttttgcatct tccaccagta ctcgaactcg gcatcccact tgcccagcgc cgtggcgaga
     3841 cggtagtcct gatactcgga cagccaggct tgccggtata gggctttcag ctccatcgtg
     3901 gagtccatca ggtcctgaag caggccgtgg ttatgagagc cggtttgggt cctcaagagc
     3961 agattcacct tgtcacgcgg cgcatccgga cccagcattc gaaagtactt ggcaatctcg
     4021 atcgcgtaca gattcttcat gccgaggtga tccagcatgc gcgcacccaa caaaaggctg
     4081 gatagagtgg caggatcgcc cttgagcctc aacgcctcaa gcaggtgcgt ggttgcctcc
     4141 tcggccgaga ggcgtgcctc tcgaagctcc ttctcccgct gttcgagctg cttcaggcga
     4201 tcgggttcga acgggtcgtc ccagaaatgc agcatggtcg gaccgcccag tgctgtaaag
     4261 aacgtctcct gcgattttgt cagcgcagcc agaccctgag cgacctcggt cgcaacggag
     4321 ggaagataca tctggcgggc atagtcagag aagaactgct cttgatcgac cggagacgat
     4381 tgccagggag ctgccgcgcc gaaggccatg ccgggaagcg acatccggta aatcgtctgg
     4441 gcgtcatcgc tccaacccgt attgaggagg cccatggcgc cgtacttccg gcccgcggca
     4501 agcataccac tgatgtttcc gaaggacgtt tgaaaacttg gcgccacctc gttccagttc
     4561 cagacgcccg gagcgacaac catcggcaag ccggccttcg ccagcggctc aacgatcggt
     4621 gtgaagtcct tctcaacccg gtagtgccag ggcactgcga ttgtttcctt gggaagctgg
     4681 gaaatcaact ccggatagcg ggagaaaatc cgcgccccgg cgatcatgtc cgcccagaac
     4741 atcatccgct tgttgtgatt cagcacaatg gcggagacgt tcttgagatg ctccatgaag
     4801 agctccgccg gcggtgcacc ggccacaggg ctgccgaaga gttctaacgc ccacggttcg
     4861 tcaaagccga tgtgaaacca gggactggga aacaactgag ccatctggcc cacccagtca
     4921 tcgaggaacg ctcggacccg cgggtgccgt ggattgaatt cgcctccgta ccgcagcggg
     4981 gccatgtcgg agtactgctc gacgcggaac acgtcgtgca tgtgcccgta gaattccatg
     5041 cagggcacaa cgtcgatatg gcgctggcgg gcatagccga tgatccgttt gatctgttgc
     5101 tgggagtagc gggattccag gttcaccaat gggtaacctc ggacttcgat gttggcttcg
     5161 gcgtagaaat agtattggtt tgccttccac ctcgccagga aatcgagttg ccgcttgatc
     5221 tcatcctcgg taagcagtgc tccgtggttg gtgtccatca tgaagccgcg gtaggcgagc
     5281 gacggccagt cccggatctc cacttcgggg agcgccgcct gtgaaccgga accttccacc
     5341 aactggcgga atgtctggac gccatagaac aagccggcgg aggattttgc gcggatctcg
     5401 gcgccttccg cggtgatctt gaggctgtac gcttcgcgcg agtccggccc cccgcgttcg
     5461 tcgcgcatgg gaagcgccgg agcattcccc gtttgcgcga gtacgatcgc ccgacccgaa
     5521 cctgagcctt ccgtaaccga aacactcgac tgcgctgccg cagacagtgc cgacgacagt
     5581 tccctcgcgg caaacttatc ctccgcgccg ggcgtggagg ctagccggat tgtcaggccg
     5641 cgcagcgaca gttttcccgg tccgtatcgg acttcctgcg ggcgaggaag caacgggtta
     5701 tggctggccg ccgcagcggg cagcagcacg acgagcagtc caatgatggc gcgcatattc
     5761 tttattcctc ggatttccag attatgaacc ccgtcggagc agaaccccct ccgcgctcac
     5821 attcaggatg tgccgaccca ggcccctcgg aacaacgact gtctgcttct cgggctgctg
     5881 tgatttcagg ctgcttcgca gttcgtcgga aaggtggagg gtccacgttg attcgtgaga
     5941 gtcaaagtgg cggcagtaat ggtcgtcgta catcagcgac agcgccagcg attcggatgg
     6001 agacgggtcc cgggcgactg tggagtgcag atacgcgcag tattcgccgg cagtcatgta
     6061 tcgcacgccc ttgccgagat cgttcagcag acgctctatc gcgcccttgt ccattgcgac
     6121 atcccgatcg tgaaacgcga t
//
LOCUS       DATIFQ010000386        10074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2426280, whole genome shotgun sequence.
ACCESSION   DATIFQ010000386 DATIFQ010000000
VERSION     DATIFQ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2426280"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VLE22_18670"
     CDS             complement(<1..761)
                     /locus_tag="VLE22_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16482.1"
                     /translation="MTFRVRLILSVALTVAVTVTLAIWAVSSFTERAFGELDDQRTKA
                     LVAQFRREFARRGEEVVRTSEAVAASESALRMVVEARRRQPDYSPYVQEAGTLAAAQR
                     LDFLEIVAGDGSIISSAQFAARFGYKADWLLRLTGWQKTGAFLKREELPGEMALALTA
                     VREVMAGDRKLYVVAGRRLDKEFLGSLVLPAGMRVLLYRNFTASFAQDSLTDPLGAVA
                     GASTLEGLIEEVRTHKREVSRTIQWPDGPETFQAIP"
     gene            complement(758..2095)
                     /locus_tag="VLE22_18675"
     CDS             complement(758..2095)
                     /locus_tag="VLE22_18675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSB16483.1"
                     /translation="MRHTACRLVALGSFLALLSIAADRPRYGGTLRLAMTDARAKEIL
                     SPLVFERLVDFHGGQAKATLAVSWVHNTPRTRWRFRLRPDVRFHDGTPVSAEAVAGSL
                     ASGKNDWRAFTSGGDIIIQASRPIPGLLWELAESRPPVVVRNPDGAEKGTGPFRISEW
                     QEGSRAVLTANNGHWAGRPFVDTITVELGRSSRDPVLDLELGRTDVAEVDPAEARRAS
                     ERGLRVWSSAPLELWALRFEAGRSAGANDALREAVAFAIDRPAIHAVLLQKRGEPAGG
                     ILPQWISGYAHLFPSGRDIERARRAAASTPAALRSIVLVHGADPMERAVAERIALNAR
                     EAGISVRTTGSGTADLRLVRVRITSVQAGGALGSIADALRIPDFEPLPATASLDAIFR
                     AEQALVEQRVVIPLFHLPELYATSPEVKVWTTPPVSRDGTLRLDSVWLARERP"
     gene            complement(2058..3194)
                     /locus_tag="VLE22_18680"
     CDS             complement(2058..3194)
                     /locus_tag="VLE22_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16484.1"
                     /translation="MSHSFLRRLAMFLIWIAPFAMSSAQEPESAARELARRIAGSLSP
                     GEAVSLAIENRSSLPPAAVVEARRAVEAALRAAGAGLRSDGTKATITLSENASGQVWT
                     AEIMRGDRREVFLVIGDSSATSRGQFRAVLEAAILIEAAEPILDVASVSDSLLVLQPG
                     EVSIYERNDKVWERRQASPIPNAPVWPRDVRGRLTLEGETFRAYLPGLTCAGAMQPTL
                     VFECNEGNRWPAGFGGAEFATGRNHFTGDGAVSFFSAAVVGSESGNLRVLAGVDGRTY
                     VLDGAMRRLNVFDGWGSDVTAIDSACGRVVLASGSGEPVGQDTVRAYELIDGHPAAAS
                     RPLHFDGVVTALWPAGDGQTAIAVVQDPERQGYAAYRVSARCAR"
     gene            complement(3278..4606)
                     /locus_tag="VLE22_18685"
     CDS             complement(3278..4606)
                     /locus_tag="VLE22_18685"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HSB16485.1"
                     /translation="MLFLKYLFLAAGAGLFAAAATIVLYDFYRGVEQWRRRDPAERGA
                     GELPPIRWQTAGKLAGLAWLPLLLGMSIVVVPSGSAGVRVSQISGTLSGTLYPGVHLV
                     VPLVQKVAVYDIRDRIYSTSVTADAKAKAEALKVQSKEGLPVGLAVAVRYRLDPRRLD
                     YVHSNLPQPVEQELVPPVVAAAFRELVPNYMVREVFSSKREEVRRQAAETIRNKLAAD
                     AIVVKEVMLRDILLPGEYAKGLEGLLLKEQENERLAIEVEVKQKQVRTAELEAEAEKA
                     RRIKEAEALAQVTVLQAKAQSDAMQYTLPLKEKQIQQTKLEAEARKESTVKQAEAAAQ
                     AKVIDSKAELEKRKLMAEAEDHRIRIIAAADAERLKGEAQVLKQNPLLIQKIIAERLS
                     DKVQIMMVPTDGKYFFANDVLRSTMTLGAARGEENEPSQASFTGAMNQGR"
     gene            complement(4621..5172)
                     /locus_tag="VLE22_18690"
     CDS             complement(4621..5172)
                     /locus_tag="VLE22_18690"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HSB16486.1"
                     /translation="MDNRLMKLAAVALMAVAEGAAQQPQISRRRIVISIPDRQLALVE
                     DGRVVKIFRTAVGAPKSPSPTGTFTIMNRIPNPTWYTPGKIVGPGAENPLGTRWLGLS
                     LKGFGIHGTNSPRSIGRAKSHGCIRMRNRDVEELFEMVRDGDVVELHGERTEELARIF
                     DSASAPSEYRQIAVARPPAPENF"
     gene            complement(5285..6367)
                     /gene="amrS"
                     /locus_tag="VLE22_18695"
     CDS             complement(5285..6367)
                     /gene="amrS"
                     /locus_tag="VLE22_18695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HSB16487.1"
                     /translation="MATLVEDLERSVREGILWTPLEGKRIRCVACGHRCPIPEGQPGV
                     CKVRFNQGGKLYVPWGYVTSVQCDPIEKKPFNHVLPGALAFSFGMLGCDLHCSYCQNW
                     VTSQALRDPRASAPLRRVPPEELVQAALTVGAAAVVSTYNEPLITAEWAVAVFQQARK
                     AGLLTGFVSNGNATPEVLEYLRPWTDLYKVDLKSFDDRHYHELGGRLQPILDSVRLIH
                     RMGFWLEVVTLVVPGFNDSDDELRSIAEFLAGISQDIPWHVTAFHKDYKMLDPDNTPA
                     ETLTRAALIGRQAGLRYVYAGNLPGSVGELEDTRCPGCGECLVRRRGYRILDHRVTPE
                     GRCPSCQTVIPGIWAERVTVDTASNA"
     gene            6662..7141
                     /locus_tag="VLE22_18700"
     CDS             6662..7141
                     /locus_tag="VLE22_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16488.1"
                     /translation="MDSDRKYSQRGYMDSDRDKSQVGRREEKPRQQGPRLPIDVTGPR
                     LPRLVQTVVASRCYNCAVTLPAGIDFKGNCPKCNVALHCCKQCAHFDPSKRFQCVKPI
                     PERIAVKDQPNQCGLFEPRVTVARDVTPTGASPVSGVTSAPRGPNEARAAFDNLFKK"
     gene            7197..8231
                     /locus_tag="VLE22_18705"
     CDS             7197..8231
                     /locus_tag="VLE22_18705"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSB16489.1"
                     /translation="MRIAELSESHRFQLVDGPIREPGPGEVQVRVDSVGICGSDLHYF
                     AEGGIPGAPCVYPMVIGHEPAGEIVRTGPGVTGWSPGDRAALEPAHYCYHCEFCITGH
                     HNVCENIRFLSTPGEPGFFREFVNLPAANALPLPPTLGFREATLFEPLAVILHSMKFA
                     AIAPGESAVVFGAGPIGLLTIAMLKMAGCGRLWAVEPVAHRRDLARLLGADEAVDPHA
                     ADPVAEILRDTGRRGVDVSFDCAGRDDTINQCIHLTRSAGRVVLTAIFPEPRVPVEFH
                     VVRRKELVMYNVRRSSHESETAIELLHRYPERFVPMITHVRPLEEIQRVFDTLERYAD
                     GIGKPVISLR"
     gene            complement(8241..8798)
                     /locus_tag="VLE22_18710"
     CDS             complement(8241..8798)
                     /locus_tag="VLE22_18710"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSB16490.1"
                     /translation="MTFPELYKTKLLKTLDVIPLDKVVAAIEWFKEARAAGRTIFVCG
                     NGGSAATASHFVCDIVKGASYNRDSRFRIQALTDSLSTITAYANDVSYDIVFAEQLKN
                     FGRPGDIVMCISGSGNSPNVLRAMEYANSIGCRTLALTGRDGGKLGPMAQLDIRVPEP
                     HMGRIEDAHMIVCHMIGYFFMEERS"
     gene            complement(8795..9652)
                     /locus_tag="VLE22_18715"
     CDS             complement(8795..9652)
                     /locus_tag="VLE22_18715"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSB16491.1"
                     /translation="MNTLAIDVGGTKFSMAVFVDGRMAARETRATDREGGREWMLDQI
                     ASIAAEWERTYHLEKCGIGFGGPVEYAKQRVARSTHVGGWLDFALPAWIERALQVPTL
                     MDNDANVGALGEAVYGAGAGCDPLFYMTISTGIGGGLIAGGKIYRGADSYAGEIGHLT
                     VRPGGRQCLCGAFGCLERSCSGLWLERDYGRSAKELLQDSDFVTGYVVDLAIGLKSCI
                     MLLNPARIVIGGGISKAGDALFEPLRAELRLQVTDWSKARIDVVPAALGDDSVLYGAL
                     ALAKEIPVQ"
     gene            complement(9649..>10074)
                     /locus_tag="VLE22_18720"
     CDS             complement(9649..>10074)
                     /locus_tag="VLE22_18720"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSB16492.1"
                     /translation="RKILFADSRLRIEGFRGVIAKPNRQEAEAACGRVFGAVEYERLR
                     RVIEAPLLIVTLGADGALLVGGQGTERVSTKPVENPVDICGAGDSFSAGSALALAAGA
                     SPAQAVRFGNLVTSVTIMKKGTGTASPEEVLAAEEALVP"
BASE COUNT         1755 a         3459 c         3003 g         1857 t
ORIGIN      
        1 gggatcgctt gaaacgtctc cggcccgtcc ggccattgaa tcgtccggct gacctcccgt
       61 ttgtgcgtgc gtacctcctc gatcaacccc tcaagggttg acgcgcccgc aaccgccccc
      121 agcggatcgg taaggctgtc ctgcgcaaaa ctggccgtga agttgcggta cagaaggacg
      181 cgcattccgg ccggaaggac cagcgagccg agaaactcct tgtccagccg gcgtcccgcg
      241 accacgtaca gtttccggtc gccggccatc acttcacgca ccgccgtcag agccagcgcc
      301 atttcgccag gcagttcctc tctcttcagg aaggctcccg ttttctgcca gccggttagc
      361 cgcaagagcc agtcggcttt gtagccaaaa cgcgccgcga attgcgccga cgagatgatc
      421 gagccgtcgc cggcgacgat ctccaggaaa tccagtcttt gggcagcggc gagcgttccc
      481 gcttcctgca cgtagggaga ataatccggc tggcgccggc gggcttcgac taccatcctt
      541 agtgcgcttt ccgacgccgc aaccgcctcg gacgtgcgga cgacctcctc gccccgccgc
      601 gcgaactcgc gtcggaattg cgctaccagg gccttggtcc gctgatcgtc cagctcgccg
      661 aaggcgcgtt ccgtgaacga ggagacggcc cagatcgcca gcgtcactgt gaccgcgacg
      721 gtcagcgcga ccgacagaat gagccgaaca cggaacgtca tggccgttcc ctcgccagcc
      781 agacactgtc cagccgcagt gtgccgtcgc gggagaccgg gggagtcgtc cagaccttga
      841 cctccggcga cgtggcgtaa agctccggca gatggaatag cggaatcacc acgcgctgtt
      901 cgacaagcgc ctgctcggca cgaaaaatcg catccaggga agcggtcgcc gggagcggtt
      961 cgaaatcggg aatcctcaga gcgtcagcta tgctcccaag tgcccctccc gcctggacgc
     1021 tggtgatccg cacccgtaca aggcggaggt cggccgttcc cgagcctgtt gtgcggacgg
     1081 agatgccagc ctcacgcgcg ttcagcgcga ttcgttccgc cactgcccgt tccatcgggt
     1141 ccgcaccgtg cacaagcacg atcgaacgaa gcgccgccgg tgtcgaagca gccgcccgcc
     1201 gcgcccgctc gatgtcccgc cctgagggaa acaggtgggc atagccgctg atccactggg
     1261 gaaggattcc gcccgcgggt tccccgcgtt tctgcagcaa tactgcgtga atggccggac
     1321 ggtcgattgc gaaagcgact gcttcccgca acgcgtcgtt tgcgcccgct gaccttcccg
     1381 cttcgaatcg caaggcccac agctcgagcg gcgcgctgct ccagacgcga aggccgcgct
     1441 ccgatgcgcg tcgcgcctcg gcgggatcca cctccgcgac atcggttcgc ccaagctcga
     1501 ggtccaatac gggatctcgg gacgagcggc ccagttcgac ggtgatcgtg tcgacaaagg
     1561 gcctgcccgc ccagtgcccg ttgttggcgg tcagaacggc gcggcttcct tcctgccact
     1621 ccgagatgcg gaagggccct gtgcctttct cggcgccgtc agggtttcgc accacaactg
     1681 gaggacgtga ctcagccagt tcccacagca gccccggtat cggacgcgag gcctggatga
     1741 tgatgtcccc accggacgtg aaggctcgcc agtcgttctt ccccgacgcc agtgacccgg
     1801 ccacagcttc cgcgctcacc ggcgtaccgt cgtggaacct gacgtccggc cgaagcctga
     1861 accgccagcg cgtccgcggt gtgttgtgca cccaggagac ggcgagcgtt gctttcgctt
     1921 gcccgccgtg gaaatccacc agccgttcga acacaagcgg agacagtatc tccttggctc
     1981 gcgcatcggt catcgccagg cgaagagttc ctccgtaacg cgggcggtcg gcggcgattg
     2041 aaagcagcgc cagaaagcta ccgagcgcaa cgagccgaca cgcggtatgc cgcataccct
     2101 tgcctctccg gatcctggac gacggcgatc gcggtttgtc cgtctcccgc cggccacagc
     2161 gccgtgacga cgccgtcgaa atgaagcggc ctgcttgccg ccgcgggatg gccatcgatc
     2221 agttcgtacg cccgaaccgt gtcctgccca accggttcgc cgctcccgga cgccagcacc
     2281 acccggccgc acgccgagtc gatcgcagtg acgtcgctgc cccatccatc gaatacgttg
     2341 agccggcgca tcgcgccgtc caacacgtag gtccgaccgt ctactcctgc cagcacacga
     2401 aggttgcctg attcggagcc aactacggcg gcggagaaaa acgaaaccgc tccgtcgccg
     2461 gtgaagtgat tccggcccgt tgcaaactcg gcgccgccga agccggccgg ccaccggttt
     2521 ccctcgttgc actcgaaaac gagcgtgggt tgcatcgcgc cggcacaggt gaggcctggc
     2581 aaataggctc ggaacgtctc gccttccaac gtcagtcgtc ctcggacgtc ccgtggccaa
     2641 actggcgcgt tcggtatcgg tgaagcctgc cgccgttccc agaccttgtc gttacgctcg
     2701 tagatcgaca cctcgcccgg ctgaagcaca agcagactgt cgctcacact ggccacgtcc
     2761 agaatcggct ccgccgcctc gatcaggatt gccgcttcca acaccgcacg gaactgtcct
     2821 ctggaagtcg ctgagctatc tccgatcacc aggaacacct cccgtcgatc gccccgcatt
     2881 atttcggcgg tccaaacctg ccccgacgca ttctccgata gggtgatcgt cgctttcgtt
     2941 ccgtcgctcc gaagtccggc tccggccgcc ctgagggccg cctcgacagc acgacgcgcc
     3001 tccaccacgg ccgcgggagg gagcgacgac ctgttttcga tcgcgagcga caccgcttcg
     3061 cccgggctca ggctcccggc gattcggcgg gccagttccc gggcggcgct ttcgggttct
     3121 tgcgcggatg acatcgcgaa cggggcgatc catatcagga acatcgccaa ccgccgcaaa
     3181 aaggagtggg acatgcgccg tttgtccctt cagcatatac cagggtcaaa cacgcatgtc
     3241 ccgccagtgc cacaaacctc ggcccatgct tcgccggtta cctcccctgg ttcatcgctc
     3301 ccgtgaaact cgcctgggaa ggttcattct cttcgccccg ggccgccccg agcgtcatcg
     3361 tcgacctcag cacgtcattg gcaaagaagt acttgccgtc ggtcggaacc atcatgatct
     3421 gcaccttgtc cgacaggcgc tcggcgataa tcttctggat cagcaacgga ttctgtttga
     3481 gcacctgcgc ttcgcccttg agccgctccg cgtctgccgc ggcgatgatg cgaatgcgat
     3541 ggtcttcggc ttcggccatc agctttcgct tttcgagttc cgccttgctg tcgatcacct
     3601 tggcttgcgc cgccgcctcg gcctgtttca ccgtggactc cttgcgtgct tcggcctcca
     3661 gcttcgtctg ctggatctgc ttctccttca gcggaagggt gtattgcatc gcatccgatt
     3721 gcgctttggc ttgcagcacc gtcacctgtg ccagcgcttc ggcctccttg atgcggcggg
     3781 ccttctcggc ttcagcctcc agttcggctg tgcgcacctg cttctgcttc acctcgacct
     3841 cgatcgcgag ccgttcgttc tcctgctcct tcagcagcag tccctcgagt cccttcgcgt
     3901 actcgcccgg cagcaggatg tcgcgcagca tgacctcctt cacaacgatc gcgtctgccg
     3961 ccagcttatt cctgatcgtt tcggcagctt gtctgcggac ctcctcgcgc ttcgacgaga
     4021 agacctcccg caccatgtag ttcggaacga gctcccggaa cgccgcggcg accaccggcg
     4081 gcaccagttc ctgttcgacc ggctgcggaa ggttcgagtg gacgtaatcg agccgccgcg
     4141 ggtccagccg gtaacggacc gccacggcca acccaaccgg cagaccctct ttggactgca
     4201 ccttcagcgc ctcggccttc gctttggcat ccgccgtcac agaagtcgag taaatccgat
     4261 cgcggatgtc gtagacggcc actttctgca ccaatgggac caccaggtgc acgcccggat
     4321 acagggtgcc gctcagcgtc ccggatatct ggctcacgcg aacgcccgcg ctcccgctcg
     4381 gcactaccac gatgctcatc ccgagcagca gaggaagcca ggccaacccc gccagctttc
     4441 ccgcagtctg ccagcggatc ggtggaagtt cccccgctcc gcgttctgcc ggatccctcc
     4501 tccgccactg ctcgacgccg cggtagaagt catagagaac gatggtggcc gcggccgcaa
     4561 acagcccggc gcccgccgcc agaaacaggt acttgagaaa cagcatggtt tgatcctcct
     4621 tcagaagttc tcaggggccg gaggccgtgc aacggcgatc tggcgatact cgctaggagc
     4681 tgaggcgctg tcgaagatcc gcgcgagctc ttcggtgcgc tcgccgtgaa gttcgaccac
     4741 gtcgccatcg cggaccatct cgaacaactc ttccacatcg cgattcctca tgcggatgca
     4801 gccgtgtgat ttagcgcgcc cgatcgagcg cggactgttg gtgccgtgaa ttccgaagcc
     4861 cttcaagctc aggcccagcc accgcgtgcc gagcggattc tccgcacccg gccctacgat
     4921 cttgcccggt gtgtaccagg ttggattcgg gatccggttc atgatggtga acgttccggt
     4981 ggggctgggg ctcttcggtg cgccgacggc cgtgcggaag atcttcacca cacggccgtc
     5041 ttccaccagc gccagctggc ggtcgggaat gctgattacg attctgcgtc gtgagatctg
     5101 cggttgctgc gctgctccct ccgccaccgc catcagagcc accgccgcca gcttcatgag
     5161 tctgttgtcc attcgccgca ttgccgtttc ctccagctca cacgacgaat tggcacgggt
     5221 ggttccaatc tctaactatt tgatttattg agagttactt gatgacggtg ggctccgggc
     5281 ggtgtcaggc gttagacgcc gtgtctacgg tgacacgctc cgcccagatg ccggggatca
     5341 ccgtctggca ggaggggcag cggccttcgg gggttactcg gtggtcgaga atccggtacc
     5401 cccgccgcct taccagacac tcaccgcaac cggggcagcg agtgtcttcc agctcgccca
     5461 ccgatcccgg aaggttgccg gcgtaaacgt accgcagtcc cgcctgccgg ccgatcaaag
     5521 ccgcgcgggt cagagtctcc gccggcgtat tgtccgggtc gagcatcttg tagtctttgt
     5581 ggaatgccgt gacgtgccac gggatgtcct gcgagatccc ggcgagaaat tccgcaatgc
     5641 tccgcagttc gtcgtcggag tcattgaacc ccggcaccac gagcgtgacc acttcgagcc
     5701 agaagcccat ccggtgaatc aggcggacac tgtcgagaat cggctggagc ctgccgccga
     5761 gttcgtggta gtgccggtcg tcgaagcttt tcaggtccac cttgtacagg tcggtccacg
     5821 gccgcagata ctccagcacc tccggcgtcg cgtttccgtt ggaaacgaag cccgtcagca
     5881 gacctgcctt gcgcgcctgc tggaacacgg cgaccgccca ctcggcggtg atcaacggtt
     5941 cgttgtacgt gctcaccacc gcagcagctc cgacagtcag ggcagcttgc acgagttcct
     6001 ccggcggtac acggcgaagc ggggccgacg cgcgggggtc gcgcagcgcc tgggaggtca
     6061 cccagttctg gcagtaggag cagtgcagat cgcagcccag catgccgaaa ctgaaggcca
     6121 gcgcgccggg cagcacgtgg ttaaacggct tcttttcaat cgggtcgcac tgcacgctgg
     6181 tgacgtagcc ccacgggacg tagagcttgc cgccctggtt gaatcgcacc ttgcagacgc
     6241 cgggttgccc ttcgggaatc gggcaacggt gcccgcacgc gacgcagcgg atgcgtttcc
     6301 cctccagcgg cgtccagagg atgccctctc ggacgcttct ctcgagatcc tccacgagag
     6361 tcgccattcc acccctagta tcgctctttg ggacaacacg atggcccacg ggtcaaaaag
     6421 gaacactaga ctctgttacc cggttgtggt acacatagat ttgaggcttt ggctgggaac
     6481 ccgtccgagc gagttttccg gctttgcttc agactttcct ctgatgaccc tgcgctgatg
     6541 agggcggggt tcttgaacaa gctccacact gaggtccttc gtatgaggtc ctggagcacc
     6601 ccgacggatc gtcaggagcg agctcaccag acccaatact tccaggagcc tgcatccggc
     6661 catggacagt gatcgcaaat actcacaacg cggctacatg gactccgacc gtgacaagtc
     6721 ccaggtaggg cgaagagaag agaagccacg gcaacagggg ccccggctgc cgattgacgt
     6781 gaccggcccc cggctgccgc ggctcgtgca gaccgttgtc gcttcacggt gttacaactg
     6841 tgccgtgact ctgccagccg gcatcgactt caagggcaac tgcccgaagt gcaatgttgc
     6901 cctgcactgc tgcaaacagt gtgctcattt cgacccctcc aagcgcttcc agtgtgtcaa
     6961 accgattccg gagcgcatcg cagtgaagga ccagccgaac cagtgtggac tgttcgagcc
     7021 tcgggtcact gtcgcacggg acgtgacgcc cacgggcgct tcgccggtct ccggggtcac
     7081 ttctgcgccc cgcggtccca acgaggcgcg ggcggccttc gacaacctct tcaagaaata
     7141 gttcgctcgc acagtgaacg cagagttgga ctctacacgg gcaaggacac ggatctatgc
     7201 gcatcgctga gctctccgaa tctcaccgct tccagttggt cgacgggccg atccgggagc
     7261 caggtcccgg cgaggtccag gtgcgagtcg attcggtcgg catctgcggc tcggacctgc
     7321 actacttcgc cgagggcggc atccccggcg ccccgtgcgt ctacccgatg gtgatcggcc
     7381 acgaaccggc cggagagatc gtcagaacgg gtcccggcgt gaccggttgg tcgccaggag
     7441 accgtgccgc gcttgagccc gcgcactact gctaccactg cgagttctgt atcaccggac
     7501 atcacaacgt ctgcgagaac atccgctttc tcagcacacc gggcgaaccg ggcttcttcc
     7561 gggaattcgt caaccttccc gcggcgaatg cgctccccct gccgccgaca ctcggcttcc
     7621 gggaagccac cctgttcgag ccgctggccg tcattcttca ctcgatgaag ttcgcggcca
     7681 tcgctccggg cgagagcgca gtcgtgttcg gagcggggcc gatcggactt ctcacgatcg
     7741 ccatgctcaa aatggcgggc tgcggccgct tatgggcggt cgagcccgtc gcccaccgcc
     7801 gcgatctggc gcgcctcctc ggagccgacg aggccgtgga cccccatgcc gccgaccccg
     7861 tggcggagat cctccgcgac accggccggc gcggcgtgga cgtgtccttc gattgcgccg
     7921 ggcgcgacga caccatcaac cagtgcattc acttgacccg gagcgccggg cgcgtggttc
     7981 tcaccgcgat ctttccggaa ccgcgtgtgc cggtggaatt ccacgtggtg cgccggaagg
     8041 aactggtgat gtacaacgtg cggcggtcga gtcacgaatc ggagaccgcg atcgagctgc
     8101 tacaccggta cccggaacgc tttgtcccga tgatcaccca cgtccgcccg ctcgaggaga
     8161 tccagcgggt cttcgatacg ctcgaacgct acgcggacgg tatcggcaag cccgtgatct
     8221 cgcttcgata gcccggtagg ctacgagcgt tcctccatga agaagtagcc gatcatgtgg
     8281 cacacgatca tgtgtgcgtc ctcgatgcgt cccatgtgcg gctcgggcac tcgaatgtcg
     8341 agctgcgcca tcggtcccaa tttgccgccg tcccggccgg tcaaagccag cgtccggcag
     8401 ccgatcgaat tggcgtactc catcgcccgc agcacgttcg gggagtttcc cgatccgctg
     8461 atgcacatga cgatgtcccc cggcctgcca aaattcttca actgctcggc gaacacaatg
     8521 tcatagctca cgtcgttcgc gtaggcggtg atcgtggaca gcgaatcggt cagcgcctga
     8581 atacggaagc gcgaatcccg gttgtagctg gcgcctttga cgatgtcgca gacgaaatgg
     8641 gatgccgtcg ccgcgctgcc gccgttgccg cagacaaaga tggtccgccc ggctgcccgc
     8701 gcctccttga accactcgat ggcggcgacg accttgtcga gcggaatgac gtcgagcgtc
     8761 ttaagaagct tggttttata gagttccgga aaagtcattg aacgggaatc tcctttgcca
     8821 gcgccagcgc gccgtaaagc acgctgtcat cgccgagggc ggccgggacg acgtcaatgc
     8881 gggccttcga ccagtcggtg acctgaagcc gcaactcggc tcgcaacggt tcgaacagcg
     8941 cgtcacccgc cttgctgata ccgccaccga tcacgatcct tgccggattc aaaagcatga
     9001 tgcacgattt caacccgatc gcgaggtcga ccacgtagcc cgtcacgaaa tcggaatcct
     9061 ggagcaattc cttggcgctg cgtccgtaat cgcgctcgag ccaaagcccg ctacagcttc
     9121 gttcgaggca tccgaacgcg ccgcacaggc actgccggcc gccgggccgg accgtcaggt
     9181 gccctatttc gccggcgtac gaatccgcgc cccgatagat ttttccgccg gcgatcagcc
     9241 cgccgccgat gccggtcgag atggtcatgt aaaagagcgg gtcacacccg gcgcctgcgc
     9301 cgtacaccgc ctcgcctaaa gccccgacgt tggcatcgtt gtccatcaat gtgggtactt
     9361 gaagagcccg ctcgatccat gccggcagcg cgaaatcgag ccagccgccg acgtgcgtgg
     9421 atcgcgccac ccgctgcttg gcgtattcca ccggtccgcc gaagccgata ccgcacttct
     9481 caagatggta cgtccgctcc cactcggccg cgatcgaggc aatctgatcg agcatccact
     9541 cccggcctcc ctcgcggtct gtggcgcggg tctctctcgc ggccatgcgg ccgtccacaa
     9601 acacggccat ggagaacttg gtcccgccga cgtcaatggc cagggtattc acggcaccaa
     9661 ggcctcctct gccgcgagca cttcttcggg cgaagctgtg ccggtcccct tcttcatgat
     9721 ggtgacggag gtgacgaggt taccgaagcg caccgcctgg gccggcgacg ctccggcggc
     9781 gagcgccagg gccgacccgg ccgaaaaact atcgcccgcg ccgcagatgt ccacgggatt
     9841 ctcgaccggc ttcgtggaga ccctctccgt tccttgcccg ccgacaagca atgcgccgtc
     9901 ggcgccgagg gtgacgatca gcagtggcgc ttcgatcact cgccgaagcc gttcgtattc
     9961 caccgcgccg aacactcttc cgcaggcggc ctcggcttcc tgccggttcg gcttggcgat
    10021 gacgccgcga aacccttcaa tgcgaagccg cgagtcggcg aacaggattt tcct
//
LOCUS       DATIFQ010000387        18166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2811152, whole genome shotgun sequence.
ACCESSION   DATIFQ010000387 DATIFQ010000000
VERSION     DATIFQ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 18166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18166
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811152"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VLE22_18725"
     CDS             <1..285
                     /locus_tag="VLE22_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB16493.1"
                     /translation="WADNITYVTKQLPNEPAFSARKYLPDIAPLSLFMIHSSKDEYTP
                     AKDAQKLFEAARQPKRLSVIEAQNHRFVGNHEEFFRVLREAVEWVRLATR"
     gene            261..1307
                     /locus_tag="VLE22_18730"
     CDS             261..1307
                     /locus_tag="VLE22_18730"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSB16494.1"
                     /translation="MGPPRDPLGLAATGRAGTVERFLKPAAGYLLGAACLAWLFHDVH
                     PREVLRTASGMNWGWVGVAICFDIASYVSQGWRWKLLLAPLGRIGTLRATQAIYVGLF
                     ANEVLPMRAGEFARTWLVSRWLASDFSAVIPSIAVERLFDGVWLAICMGLTAIFVPLP
                     RNLAAAADVLGVAVLLATALFLYLVYRKREQPPETARAALQHWKPVRLLLSLIDALEL
                     GLRKIGLSRSFYLAFGGSLLLLAGQILAFWFVMLACGLHLSVWVGAVVLLIVHLGTMI
                     PNAPANLGTYQFFVVVGLGVFGVEKGAATGFSFIVFFLLTVPLWVIGLFALARTGMTL
                     TAIRREIGVQAPRT"
     gene            1620..2513
                     /gene="deoC"
                     /locus_tag="VLE22_18735"
     CDS             1620..2513
                     /gene="deoC"
                     /locus_tag="VLE22_18735"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HSB16495.1"
                     /translation="MEATELQRLVAAISEEILVRVKPPQFSDSLHSPLTADAPCKICA
                     TCPHQNVESCARHTREVVTAGAARVSSCCPVAHVDPSIASLIDHTILKPEATRGDILR
                     LCREAREHCFASVCVNPFWVPAVAAELAGSGVAVCTVVGFPLGANATGIKRAEAESAV
                     RDGATEVDMVINIGALRSGEYDTVRSDIEQVVDVSHRGGAIVKTILETALLDDNQKAI
                     ACALARMAGADFVKTSTGFGPAGATVHDVALMRLVVGPEMGVKAAGGIRTLDDLRKMV
                     EAGATRIGASASVKIVEAASA"
     gene            2525..4279
                     /locus_tag="VLE22_18740"
     CDS             2525..4279
                     /locus_tag="VLE22_18740"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSB16496.1"
                     /translation="MASAPKSATRFRERAELLDFLLEVAAATSETLDLDQLLANVADN
                     VKKVVPYELLAILLYNEKLQGLRIRYSIGHREEVVQKLVIPLGDGITGAAAELREPVL
                     VGDVRNDPRYLNALDAVRSELAVPMVARGRLVGVIDLQSTRLNAYGSYERAMLRLIAS
                     RVAATIDNARLYRRVDRQNRTLRTLARLSQGFSSILDLDELLGRIADTVRSLIPSDSF
                     SILLLDQRAKVLRHRFSRRVDERGSLDNIPLGRGITGAAAESKETIRVPDTLADPRYL
                     PFHPGIRSEVAVPLVVRDRVIGVMDLESERVGFFTEDHVRTLSLLAPQIASSIENAQL
                     YQELAERESRMESDLHAAREVQTLLLPRRPPAVEGLETAAGLLPAREISGDLYDFFVH
                     SDEHVVIAMGDVSGKGAAAALYGALVSGLLRTMAPRNCEPSLLLKSLNEALIERKVDA
                     RYVTLLVLVWHPRTGEFRMANAGAFPPLICRTGEIIKPRVEGLPLGLLDAREYEEVSF
                     ETHPGDVVVLYSDGIVDQNNLAGQDYGRRRLASLLQQHWREPAQAIVDAILIDVDRFG
                     AGAGTTDDQTVVVMKVTG"
     gene            4276..5526
                     /gene="lysA"
                     /locus_tag="VLE22_18745"
     CDS             4276..5526
                     /gene="lysA"
                     /locus_tag="VLE22_18745"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSB16497.1"
                     /translation="MNLNSDVFAYRDGTLFCEDVALADIVARAGTPCYVYSAKGILER
                     FRAYEEALRDLPHRVCYAVKANSNLAVLSLLAKAGAGFDIVSGGELFRVIRAGGDPAG
                     VVFSGVGKTADEIEYALDQGIHSFNCESENELTLIDALAARRGVKARVALRVNPNVEA
                     GTHPYISTGMREHKFGIDISEVEGVYDRSRVLPNLLIEGAGCHIGSQILDTDALIGAI
                     DVMLGLVDRLRAAGHAIRHLDLGGGLGVRYRPHEETPEIRAFIARVKEKVRGRDLFVM
                     VEPGRSIVGESGVLLTRVLYRKRTGSREFVVVDAAMNDLMRPSLYQSHHEIIPLRASS
                     RGRIRADVVGPVCESGDFLALDRELENVLPGDLLAVWTAGAYGFVLSSNYNSRVRPPE
                     VLIEGSSWCVVRSRETYEDLVRGE"
     gene            5526..7730
                     /locus_tag="VLE22_18750"
     CDS             5526..7730
                     /locus_tag="VLE22_18750"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HSB16498.1"
                     /translation="MGSSSVRPASAFARAAGRAVLGLTLLGISSLGQAAQEEGTRQTV
                     AIRTAERIRVDGVLNEKAWLEHPGVGELVQQDPQPGAAPSEPTEVRLLYNDSALFIAI
                     RCRDSQPGQLRATQMQRDGSLLFDDHIQVLLDTYHDRRNAYYFATNPAGVMVEGTISE
                     NNTPNLNWDGVWFVRTRIDSEGWTVEMEIPFKTIAFNPNTTTWGFNIARRVARLREES
                     RWTSSNLDSEFFLVARAGEVNGLEKLSQGIGLDVKPYALGGFSRDVSRADRNQMARDA
                     GVDIFYRITSNLVSSTSINTDFAETEVDTRQINLTRFPLLFPEHRQFFLEDSGIFEFA
                     ASPGTRDFTPFFSRRIGLVEGEEVPILLGQKITGKIGRFDLGLMDVKTRDSNIAPGRN
                     FFVGRLKTNFWSQSYIGALVTDGEPTGKTSNRQVGVDLKIATANLFNRGKNFRSLLYG
                     SKTSTPGMKGDDQFWGGEIVYPNDLLYLWYRGRYVGKNYFPALGFMRRTGVRESAMGS
                     NFRPRPDLWNIRQISFRFYYTQYYNLFEKQIESRQFKSTPFEIEFDSGEHFTYVFTPD
                     FERLFKPFEIRKGVVIQPGAYWFYRHRFTFETAQNRPLSFKIDGETGSFYSGDSQELS
                     PQMTWRKDAHVTTSFELQQYWVNLREGMFRTRLALFKLNYAFNPALTLSNFVQYDTDS
                     RNIGLQTRLRWMIRPGNELFFVVNHAWQQNTFDRYEAILSNVRAKLNYTFRF"
     gene            7743..9491
                     /locus_tag="VLE22_18755"
     CDS             7743..9491
                     /locus_tag="VLE22_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16499.1"
                     /translation="MRRMRGLTLVFLFLWPLSGQTVIPIDTPMSPPGWALAERALLKA
                     NADAAAEFAAKYSDARGYLRCVERWGGNDGPDDAMENFHNWTLLYALGGPQSVLDLYS
                     KIWEGHLEQYMKARAPGSEMAKDGMYYKEFITSFDWEHTGEGLAAFHFYSLAAPGDPI
                     YLQRVRRFAGFYLNEDPEAPNYDPKHKIIRSLHNGSRGPKLTPASMEDWGGLPVPGKP
                     ELLERYREASNIRGDHPLNLCASTLAFNAYALTGEKKYHDWLLEYASAWRERVEANGG
                     NIPTNIGLDGRIGGEWGGKWYGGVFGWAFDPKQSHRNYFKRGPRIAFGEAFLLTHDER
                     YIDILRRQMDNLYAAKRIENGLVLLPNKHGDAGWFGYMPSWHSDLELDLYLWSMKPSD
                     RERVATQGWIAFLEGNKPDYPLKALQAEFERVRSKVKGLREDPSTPDTRYADTPQRYN
                     PVATTALINLTLGANDPGNAGNILHSRVRYFDPVRRRAGLPDDVAALVERILPEEIVL
                     ALVNTNPVDTRELIVQMGAYREHHCDSVTVGARKIVIDAPFFRVRLAAGSGERLTISM
                     KRYAHQPTLALPWEPQ"
     gene            complement(9519..11246)
                     /locus_tag="VLE22_18760"
     CDS             complement(9519..11246)
                     /locus_tag="VLE22_18760"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16500.1"
                     /translation="MENDFFTRPFSLGNKQVQGRFVIPSGIRCTHASTIAKTFAEVAP
                     IGVVTTKSISFAPRAGYREPIYARYAEGCYINAVGLANPGAKKFLAEFDNIEIPSNKF
                     LLVSIFGGGLEEFLAAAETLSPIADGFELNMSCPHAKGYGLQIGEDVELVTAITRAVA
                     AVKPVPVFVKLAAMMRGLPETAQASMRAGAAGITVTNSIGPAMADVGEKPILYNRVGG
                     LSGDGIRPLGLRSVQQVRDAIGPDPIVIGMGGIGTAEHIAQYRKAGANLFGVGSTLTG
                     MDSAAMRTYFERLQDESQRELAPGEATSFPSITDMSYTASTLVSRDNISEGLYEIRLD
                     TLGREYAPGELAGRYFFLCVPGVGEKPFAILSAAAKTILIRTVGTFTAFLEKAPLGTR
                     ILLRGPYGAPLPRFENRNLVLVGGGTGTASLLEIAYANRYANDVRFLLGARTRDGFFD
                     LDDFREVGDVLLATDDGSEGYHGTVSDLLRHVMEAEAGWSRDRLEFVNCGPDGMVHSC
                     IAIQREVAEPEHIWGSIEYMTSCGVGICGKCASPSGSLTCIDGPFVLCDEFTPRCGSA
                     GCCHETRTA"
     gene            complement(11298..11597)
                     /locus_tag="VLE22_18765"
     CDS             complement(11298..11597)
                     /locus_tag="VLE22_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16501.1"
                     /translation="MTFVGLDEMRRLTTEVHGVQVDLSVFESVEFPDPPLPERNPADL
                     RLRGGSAAIDAGVRLPNVNDGFGGKAPDLGAYEFGQAPPHYGPRPEGMDEETSDN"
     gene            complement(11607..13016)
                     /locus_tag="VLE22_18770"
     CDS             complement(11607..13016)
                     /locus_tag="VLE22_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16502.1"
                     /translation="MRTTLLLVVCAALPTVPQDRVVPGDVTTPYPTIRNLSIEWEIQG
                     DDNLNSRVTVKYRRAGDEAWQEGMPLRRIRAGDSGNRTRPTFSWENKQSGSVFDLRPN
                     TEYEIRLNLTDPDGGSTERTVRARTRGVPRPTPGARVKQANPNNFREVEASAEAGDII
                     QLTPGYYESFTATRDGVPGRPIVIRGDTSHVVRSTFEGISLEGRKHIILEKLTVNGRV
                     SLVGAEEVAVRHCTVNAKYGITASRPPGCKNCYIADNIVTYVMPWTPEGMGDESIFGG
                     EGCVGEGIQITGPGNVICYNRVKGYRDCISTMEDRNAHEQVSVDIYNNDIYVGADDGI
                     EADFCMGNCRVMRNRLTNCFMGLSSQPSLGGPIYFIRNAMYNIIDAPFKLSRGSLGDV
                     ILHNTVVKVGDGLRMPHGPTQYGNSYFRNNLCVGGKGGGKFGRYGSGTGRAIFTPATD
                     PLNDLDYDGVGTHGTPFEG"
     gene            complement(13034..14377)
                     /locus_tag="VLE22_18775"
     CDS             complement(13034..14377)
                     /locus_tag="VLE22_18775"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSB16503.1"
                     /translation="MSEKKLEKNAGQTRRSFVQAGGAALAGLGAASGTLSARAPEALA
                     LDGGPKSVTFPVSQQSALTKWPRYGPAEKKALHDLIDSGKFYEELPIFEKEWQEYTKS
                     PFVKAHMNGTSALTSMYSALDLPPGSEIMVPSYTFFATCLAMRFFGYVPIFIDIDPST
                     ACFDLEDAKRKLTKRTRAICTMHSWGLPCEMDRISDFAKEKGLILLEDAAHAHGASMQ
                     GKMMGAWGVMGIYSFQASKVLPSVEGGMGMYQTREYFERAAAFGHYEDPPKFPQDSPV
                     RQYEGTGFGQKYRMHPFAAAVARQQLRGLDERNAVAAKNIRALNDRLLQLPGLSEPRC
                     RPDQTRVYYYASMLFLDEAKAGFKRDVLLKALQAEGVRASKWDYPEQHKMKIYSEAKW
                     WHHKPEIPAALVGTTQVNATHIFMPILYGEAPELLDQYVQAFEKVWARRNTLAKL"
     gene            complement(14400..15746)
                     /locus_tag="VLE22_18780"
     CDS             complement(14400..15746)
                     /locus_tag="VLE22_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16504.1"
                     /translation="MPSKGSLRLRRLLKWVAAAVVAAAPLCGELRVATFRADATPPNG
                     EPLIWLTPAAKVEDPLWTKGLVLADGNRRYVLCTVDWCGIGSSIHELFRRKISAAVKT
                     DVTNVAVHVVHQHTAPYVDGDSNEILSRLGRPAGLMTGRYLEDLTDRIARAAAEATRR
                     LEPFDSTGISESAVDRVASARRLFGDDGKLITRFSGGGKDPKLAALPEGPIDPKLRTI
                     TLARRGKPLARIHFYATHPQTFCCDGRVTGDFAGAAREAMEREEGVFQIYFTGCSGDV
                     TVGKYNDGTPESREQLAGRLKQGMAASAAATRFSPAGNMRWRHRELVLTPKPAEAART
                     VLRESAPHSGEQLYRAALVVAFADRKRPLEVNLLEIGKAAALFLPGEPMLEFQNYAVK
                     QAAGRFVAVAGYGDIQPGYLCTDQAFQEGGYEPSASNAGPGTEARLKAAIRALLIE"
     gene            15806..17095
                     /locus_tag="VLE22_18785"
     CDS             15806..17095
                     /locus_tag="VLE22_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16505.1"
                     /translation="MIPYSEQRRTFMKWVVAAPVFGTIAAKGLSEKLMAAAGKASSSH
                     VYTRLGIRPLINARGTWTYLSGSLLLPEVRKAMDDAARHFVDIYELERAAGKRLAELA
                     GTEFGMVTSGAAGAMAAATAACIAGTDQNNVWQLPDTTGLKHEVVMLGGRSAFDSAIR
                     LAGGKLVLVHSLDELAPALNDRTAMVYTTWLGDRLEKALAITKKAGVPMLLDDAAGIP
                     PIENLRTYAKMGIDLFCFSGGKGLQGPQCSGLLLGRKDLIDGALANYSPWEGAVCRPM
                     KVGKEEIIGVLAAVESWFKLDLNKLNAEWEKRVRRIAKLVETVPGVTTEIKIPEGGNR
                     YPTLTVNWDEQAFGLTVEQCDQQLRDGEPRIEVLTSKNPSLVPVVSEGGGKSSKSGPR
                     PNRLQIVSLTIQEGEDLLIGRRLREILGAARKRTASS"
     gene            complement(17099..>18166)
                     /locus_tag="VLE22_18790"
     CDS             complement(17099..>18166)
                     /locus_tag="VLE22_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16506.1"
                     /translation="KPGILFGINCRTVDVAAYAKTQGDEMLVVEDNEELRSTIDLLRA
                     RKIFRETSVLFPTNRGFPSVASLTGITDLQDLEARLGVKVRMLPYKALAESMETTLAD
                     NAVRQGTGAEADALIRNAVQSYLDREYVVRSLLFKRTIERLMEAHACNAFTVECFEFC
                     ASRLPEKWKITPCLIHTLFKDQGIASSCEGDMGALLGMRLLMSVSGKSSHLGNMFFRK
                     GGVVEINHSAPGIRMNGFDQPGLPYKLGRFVQSGWGTKAVVDFMQNSEKRVTVARMHP
                     NAKQVLVMKGTLVQSEGWDKDNLGCSVAAFIKPSETGNGEAFVRKQTEYGNHLVWVYG
                     DYSEAMGRLGALMGLGVEVVA"
BASE COUNT         3353 a         5842 c         5373 g         3598 t
ORIGIN      
        1 tgggctgaca acatcactta cgtcaccaaa caattgccga atgagccggc gttttccgcc
       61 cgcaaatatc tgccagatat cgccccacta tcgctgttca tgattcactc gagcaaagac
      121 gagtacactc cggcgaagga cgcccagaag ttgttcgagg cagcgcggca gccaaagcga
      181 ctgtccgtga tcgaggcgca gaatcaccgc ttcgtcggca accacgaaga attcttccgg
      241 gttctccggg aggctgtgga atgggtccgc ctcgcgaccc gctaggtttg gccgctacag
      301 ggcgtgccgg gaccgttgag cgctttctca agcccgccgc cggctatctg ctcggtgccg
      361 cgtgtctcgc atggctcttc catgatgttc accctcgcga ggtgctccga accgcttctg
      421 ggatgaactg ggggtgggtc ggggtggcaa tctgcttcga tatcgcgagc tatgtcagcc
      481 aaggatggcg ctggaagttg cttctggctc cgctggggcg aattgggaca cttagggcaa
      541 cccaagccat ctacgtggga ctcttcgcca acgaggtgct tcccatgcgg gcgggcgagt
      601 ttgcacgtac atggctcgtg tcgcgatggc tcgcctcgga cttctccgca gtgatccctt
      661 cgatcgctgt tgagcggctc tttgacggcg tctggctggc gatctgcatg ggcctgactg
      721 ccattttcgt tccgctgccc cggaatctcg ccgcagccgc cgatgttctc ggcgtcgctg
      781 tcctgctggc gacagccctg ttcctgtacc ttgtctaccg aaaacgggag caaccgcccg
      841 aaacggcccg agccgccttg cagcactgga aaccggtgcg tctattgctc tcactgatcg
      901 acgcgctcga attgggactg cggaagatcg gtctgtcacg ttcgttctat ttggcgttcg
      961 gcggatcatt gctgctgctc gccggtcaaa tccttgcttt ttggttcgtc atgctcgcgt
     1021 gcggactgca tctctcggtc tgggtcggcg cggtggtgtt gttgatcgtc cacctgggca
     1081 cgatgatccc caacgccccg gcgaaccttg gcacctacca gttctttgtc gtggttggtc
     1141 tcggagtgtt cggcgtagag aagggagccg ctaccggatt ctcgttcatc gtcttctttc
     1201 ttttgacagt gccgctttgg gtgatcggcc tgttcgcgct cgcccgcacc ggcatgacgc
     1261 tcacggccat tcggcgcgag atcggtgttc aggcgccgcg aacctgacgc cgatactcgt
     1321 catggatgcc cgccgtctcg caggttgcgc ggcgtcgtca tcgacgggtc cggaatgggc
     1381 cggcaattgc agcgaactag ggttacgggt attcgcgcag ccctgtgtta tgatagggct
     1441 tctgctcgca acagtcggga atacaacgac tcgaacgcgc tcactttagg cgactgtctc
     1501 ctgacggcga gccaggccga agaggtgttg cgtgtgtggc tggcaacgcc gttcgctggc
     1561 ggcagacacc cggcccgtgt caagaaaatc tctgagatcg agcagcgata ctcaaggact
     1621 tggaagccac cgaactccag cgactcgtag ccgccatcag cgaggaaatc ctcgtccgtg
     1681 tcaagcctcc tcagttctcg gattccttgc actcaccact tacggctgac gcaccctgca
     1741 agatctgcgc tacgtgcccg caccagaatg tcgagtcgtg cgctcgacac acccgtgagg
     1801 ttgtcactgc cggcgccgcc cgcgtctcct cgtgttgtcc ggttgcgcat gtcgatccat
     1861 cgatagccag tctgatcgat cacacaatcc ttaagcctga ggcaacccgg ggcgacatcc
     1921 tccgtctctg ccgtgaggct cgggagcact gtttcgccag cgtctgcgtc aacccattct
     1981 gggtccccgc cgttgccgcg gaattggccg gatcgggcgt cgccgtttgc acggtcgttg
     2041 gcttccccct gggcgcgaac gccacaggga tcaaacgcgc cgaagccgaa tccgctgtgc
     2101 gcgacggcgc caccgaagtg gacatggtga tcaatatcgg cgctctccgc tccggcgagt
     2161 acgacaccgt ccggtccgac atcgagcagg tcgtggatgt gagccaccgc ggaggggcga
     2221 tcgtgaaaac gattctcgag acagcactgc tcgatgacaa ccagaaggcc atcgcctgcg
     2281 ctctcgcaag aatggcgggc gcggatttcg tcaagacttc cacgggtttc ggtcccgccg
     2341 gggcgacagt tcacgatgtc gctctaatgc gcctggtcgt cgggccggag atgggagtca
     2401 aagccgcagg cgggatccgc acactggacg atttgcgcaa gatggtcgag gccggcgcaa
     2461 cgcgcatcgg tgcaagcgcc agcgtcaaga tcgtcgaggc tgcctccgcc tgagcgattc
     2521 tccgatggct tccgcaccca aatccgcaac gcggttccgg gaacgcgccg aactgctcga
     2581 ctttcttctg gaggttgcag cggctacgtc agaaacgctc gacctggacc agttgctggc
     2641 gaacgtggcc gacaatgtca aaaaggtagt cccctacgaa ctcctcgcga tcctgctcta
     2701 caacgagaaa ctccagggac ttcgcattcg ctactccatc ggacaccggg aggaagtcgt
     2761 ccaaaaactc gtgatccccc tcggcgacgg tattaccggc gccgccgcgg agctccggga
     2821 accagtcctc gtgggcgatg tgcggaatga tccccgctac ctcaacgcgc ttgatgctgt
     2881 ccgaagcgaa cttgcggtgc cgatggtcgc tcgcggccgc ttggtcggcg tcatcgatct
     2941 tcaatcgacg cggctcaacg cttacggtag ttacgagcgc gccatgctgc ggctcatcgc
     3001 ctcgcgcgtg gccgccacca tcgacaatgc ccgcctttac cgtcgtgtcg atcgccagaa
     3061 ccgcacgttg cgcacgcttg cccggctttc ccagggcttt tcctcgatcc tcgaccttga
     3121 cgagttgctc ggccgaatcg cggataccgt gcggtcgctg atcccatcgg actcgttcag
     3181 catcctgttg ctcgaccaac gggcgaaggt tcttcgccac cgtttcagcc ggcgtgtcga
     3241 cgagcgcggc agcctggaca acatcccatt gggcaggggc attaccgggg ctgccgcgga
     3301 atcgaaggag acaatccgcg ttccggacac tctagccgat cccaggtacc ttccgttcca
     3361 tccaggcatt cgctcggaag tggctgtgcc gttggtggtc cgagatcgcg taatcggcgt
     3421 catggacctc gaaagcgagc gcgtcggctt cttcacggaa gatcatgtcc ggacgctctc
     3481 tctgctcgca ccacagatcg cgagctcgat tgagaacgcg cagttgtatc aggaactcgc
     3541 cgaacgcgag agccgcatgg agtccgacct ccacgccgca cgggaagtcc agacgttgct
     3601 tttgcctcgc cggccgcccg cggtggaggg tctggaaacc gccgcgggtc tgttaccggc
     3661 gcgcgagatc agcggtgacc tctacgactt cttcgtccat agcgacgagc acgtcgtcat
     3721 cgcaatggga gacgtcagcg gcaaaggagc ggccgccgca ctctatggcg ccctcgtgag
     3781 tggtctgctg aggacaatgg cgccgcgaaa ctgcgagccc tccctgctct tgaagtcgct
     3841 caacgaggct ctgatcgaga ggaaagtcga cgcccgctac gtcaccctgc tcgtactcgt
     3901 ctggcatccg cgcacagggg aattccgcat ggccaacgcc ggcgctttcc cgccgcttat
     3961 ctgccgcacc ggcgagatta taaagcctcg cgtcgaagga ttgccgcttg gacttctgga
     4021 tgccagggag tacgaagaag tctcgttcga gacgcacccg ggcgatgtcg tcgttctcta
     4081 ctcggacggc atcgtcgatc agaacaacct cgccggccag gattacggcc gaaggcggct
     4141 tgcctcgctt cttcagcagc attggcgcga acctgcccag gcgattgttg atgccatatt
     4201 gatcgacgtg gaccgcttcg gagccggcgc ggggaccacc gacgatcaga cagtggtcgt
     4261 gatgaaggta actggatgaa tctgaactcg gatgtgttcg cgtaccgcga tgggacgctg
     4321 ttctgcgaag atgtcgccct cgccgacatc gttgcccgcg ccgggacgcc ctgttacgtc
     4381 tattccgcaa aagggattct ggaacgcttc cgtgcatatg aggaagctct tcgtgacctg
     4441 ccgcaccgcg tctgttatgc agtgaaggcc aactcgaacc tcgcggtcct gtcgctgctg
     4501 gcgaaggccg gagccggctt cgacatcgtc tccggcggtg aattgttccg tgtgatcagg
     4561 gcgggcggcg atccggccgg tgtggtcttc tctggagtcg gcaagaccgc tgacgaaatc
     4621 gaatacgccc tcgatcaggg catccatagt ttcaactgcg agtccgagaa cgagttgacg
     4681 ctgattgacg cgctggccgc ccggcgcggt gttaaggctc gcgtcgcact acgagtgaac
     4741 ccgaatgtcg aagccggaac gcacccgtac atctccacag ggatgcgcga gcacaaattc
     4801 ggaatcgaca tctcggaagt tgaaggggtt tacgaccgtt cacgcgtgct gcccaacctg
     4861 ctgatcgagg gagccggctg ccacatcggt tctcagatct tggatacgga cgcgctcatc
     4921 ggcgcaattg acgtgatgct cggcctggtc gaccgcctgc gtgcggcggg acatgcgatc
     4981 cggcatctcg atctcggcgg agggctcgga gtccgatatc ggccccacga ggagactccc
     5041 gagatccggg cattcatcgc ccgcgtgaag gagaaggtcc ggggccggga cctgttcgtg
     5101 atggtcgaac ccgggcggtc gatcgtgggc gaatccggcg tgctactgac acgcgtgctg
     5161 taccggaagc ggaccggctc acgcgagttc gttgtggtgg atgcggcgat gaacgacctg
     5221 atgcggccgt cgctttacca gtcgcaccac gagattatcc ccctccgtgc ctcctcccgc
     5281 gggcggattc gggccgatgt cgtcgggccg gtatgcgaat ccggcgactt tctggctctc
     5341 gaccgtgaac tggagaacgt gctccccggt gacctgcttg ccgtctggac tgccggcgcg
     5401 tacggattcg tgctctcgtc caattacaat tcccgcgtcc gcccgcccga agtgttgatc
     5461 gaaggttctt cctggtgcgt tgtccgctct cgcgagactt acgaagacct ggttcggggc
     5521 gagtagtggg atcatcctcg gttcggcccg catcggcctt cgcacgtgcc gctggcaggg
     5581 ccgttcttgg cctcaccctg ctcggcatct catccctagg tcaggccgcc caggaagaag
     5641 gcacaaggca gaccgtcgca atccgcacag cggagcggat ccgcgttgac ggcgtcttga
     5701 atgaaaaagc ttggctggaa caccccggtg tcggcgaact cgtgcagcag gatcctcaac
     5761 ctggcgcggc gccttcagaa cccaccgaag tccgccttct ttacaacgac agcgccctct
     5821 tcatagccat ccgttgccga gactctcagc cgggacaact cagagcgacg cagatgcagc
     5881 gcgacggcag cctgctgttc gacgaccaca tccaggtcct gctggacacc tatcacgacc
     5941 ggcgcaacgc ctattacttc gccacaaacc ccgccggagt catggtggaa ggcaccatct
     6001 ccgagaacaa cacacctaac ctgaactggg acggtgtctg gttcgtgcga actagaatcg
     6061 attccgaagg gtggaccgtc gagatggaga ttcccttcaa gacgatcgcc ttcaatccaa
     6121 acacaacgac gtggggcttc aatatcgccc gccgggtggc ccgattacgc gaggagtccc
     6181 gctggacttc gtcgaatctg gacagcgagt tcttcctggt ggcgcgcgcc ggcgaagtca
     6241 acggcctcga aaaactctcc cagggcatcg gcctcgatgt gaaaccgtat gcgctcggcg
     6301 gcttcagccg cgacgtctcg cgcgccgacc ggaaccagat ggctcgcgac gccggggtag
     6361 acattttcta ccggattact tccaacctcg tgtcgagcac ctcgatcaat accgactttg
     6421 ccgaaacaga ggtcgacacg cggcaaatca acctgacccg cttcccgctt ctgttcccgg
     6481 agcatcggca gttcttcctg gaagactcag gaatcttcga gttcgccgcc agccccggca
     6541 cgcgggattt cactccattc ttctcgcgcc gtatcggttt ggtggagggc gaggaagtac
     6601 cgatccttct tgggcagaag attaccggta agatcggccg cttcgacctc ggcctgatgg
     6661 atgtcaaaac ccgtgattcc aacatcgctc cgggacggaa cttcttcgtc ggacggttga
     6721 agaccaactt ctggtcgcag tcctacatcg gggccctggt caccgacggc gagccgacgg
     6781 gcaagacctc caaccgccag gtcggtgtcg acctcaagat cgccaccgcc aatctgttca
     6841 accgcggcaa gaactttcgc tccctcctct acggctccaa aaccagcacg cccggcatga
     6901 agggagacga tcagttctgg ggcggcgaga tcgtatatcc gaacgatctc ttgtacctct
     6961 ggtaccgcgg gcggtatgtc ggcaagaact actttccggc tctcggtttc atgcgacgca
     7021 ccggcgttcg ggaatccgcg atgggcagca atttccggcc tcgccccgat ctgtggaaca
     7081 tccgccagat cagttttcgc ttctactaca cgcagtacta caatctgttc gaaaaacaga
     7141 tcgagagcag gcagttcaaa tccactccgt tcgagatcga gttcgacagc ggcgagcact
     7201 tcacctacgt gtttaccccg gatttcgagc gcctcttcaa gccttttgag attcggaaag
     7261 gcgtggtgat ccagccgggc gcctactggt tctaccgtca ccggttcacc tttgagaccg
     7321 ctcagaatcg tcctctttct ttcaagatcg acggtgaaac cggatccttc tactcaggcg
     7381 acagccagga actaagcccg caaatgacct ggcggaaaga cgcccatgtg acgacgtcgt
     7441 tcgagctgca acagtattgg gtgaacctgc gggaagggat gttccggacc cgcctcgcgt
     7501 tattcaagct caactacgcg ttcaatcccg ctctgacgct ttccaacttc gtccagtacg
     7561 acaccgacag ccggaacatc ggtctgcaga cccgcctgcg atggatgatc cggcccggta
     7621 acgagctgtt cttcgttgtg aatcacgcgt ggcagcagaa caccttcgac cgttatgagg
     7681 cgatcctctc caacgtccgg gcgaagctca actacacctt ccgtttctga ctgaagtaca
     7741 ttgtgagacg catgcgcgga ctcacgttgg ttttcctctt cctgtggccg ctctcaggcc
     7801 agacggtcat cccgatcgat acccccatgt ccccgcccgg atgggctctc gccgaacggg
     7861 cgctgctcaa agcgaacgcc gatgccgcgg ctgaattcgc tgcaaaatac agcgacgctc
     7921 gtgggtacct gcgctgtgtc gaacgctggg gcggtaacga cggtccagac gacgcgatgg
     7981 agaacttcca caactggaca ctgctctacg cactcggagg gccgcagtcc gtcctcgacc
     8041 tgtacagcaa gatctgggaa ggccacctgg agcaatacat gaaggcacgt gcgccgggga
     8101 gcgaaatggc caaggatggg atgtactaca aggagttcat tacctccttc gactgggagc
     8161 acaccggtga ggggctggcc gcgtttcact tctatagtct cgccgcgccc ggcgatccca
     8221 tctatctgca gcgtgtgcgc cgttttgccg gcttttacct gaatgaggac ccggaagcgc
     8281 cgaactatga cccgaaacac aagatcatcc gcagtctgca caacggtagc cgcggaccga
     8341 agctgacgcc ggccagcatg gaggactggg gtggccttcc ggtccctgga aaaccggaac
     8401 tgctcgagcg gtaccgcgaa gcctcgaaca tccgcggtga tcaccctctc aacctctgcg
     8461 cctccacgct ggcattcaac gcctatgcgc tcacgggaga gaagaaatac catgactggc
     8521 tgcttgagta tgcctccgcg tggcgggaac gggtggaggc caacggcggg aacattccga
     8581 cgaacatcgg tctcgacggc cgcatcggcg gtgagtgggg tggtaaatgg tacggcggcg
     8641 tgttcggatg ggccttcgat ccgaagcaaa gtcatcggaa ttacttcaaa cgcggaccgc
     8701 gcatcgcctt cggcgaagcg ttcctgctga ctcacgacga gcgatacatc gacatcctgc
     8761 gccggcagat ggacaacctc tacgctgcga agcgcatcga gaacggcctc gtccttcttc
     8821 ccaataagca cggcgacgcc ggctggtttg ggtacatgcc gagctggcac tcggatctgg
     8881 aactggattt gtatctctgg tccatgaagc cttccgaccg ggagcgtgta gctacgcaag
     8941 gctggattgc gtttctagag ggaaataagc cggactaccc attgaaggcc cttcaagcgg
     9001 agttcgagag ggttcgctcg aaggtcaagg gactgcggga agatcccagt acgccagaca
     9061 cgcggtatgc cgacacgcca cagcgataca atccggtggc cacaaccgcg ctgatcaacc
     9121 tgacgttggg agccaacgac ccgggaaacg ccggcaatat cctgcacagc cgcgtccgct
     9181 actttgaccc cgttcggcgc cgggcggggc tgccggacga cgtggcggcg ctggtcgaaa
     9241 gaatcctacc cgaagagatc gtgcttgcgc tggtgaacac gaacccggtc gatacccgtg
     9301 agctgatcgt gcaaatgggc gcttaccggg agcaccattg cgatagcgtg accgtcggag
     9361 cgcggaagat tgtcatcgac gcccccttct ttcgcgtgag acttgcagcg ggctccggtg
     9421 aacgactgac gatctccatg aagcggtacg ctcaccaacc cacactcgcg ctcccatggg
     9481 aaccgcagtg atcttgccca cggcgagtcc tcgctgactc aggcggtccg tgtctcgtgg
     9541 cagcagccgg ccgaaccgca gcgcggcgtg aactcgtcgc ataggacgaa cgggccgtcg
     9601 atgcatgtca ggctcccgga cggagaagca cacttcccgc agatcccgac gccacaactt
     9661 gtcatgtatt cgatggagcc ccagatatgc tccggctcgg cgacctcccg ctggatcgca
     9721 atgcaggaat ggaccatacc gtcgggtccg cagttcacga actcgaggcg atcccggctc
     9781 catcccgcct cagcttccat gacgtgacga aggagatctg acacggtgcc atggtatccc
     9841 tcggaaccgt cgtcggtcgc gagtagaacg tcgccgacct cgcggaagtc gtcgagatcg
     9901 aagaacccat cgcgggttcg cgcgccgagc aaaaaacgga catcatttgc gtaccggttc
     9961 gcgtaggcga tttcgagcag agaggcagtg cctgttcctc cgcccaccag caccaggttc
    10021 cggttctcga aacggggcaa cggggcgccg tatggcccgc gcagaaggat ccgcgtcccg
    10081 agcggcgcct tctccaggaa ggccgtaaac gtgcctacgg tgcgaatcag gatggttttc
    10141 gccgcagcgg aaaggattgc gaaaggcttc tcgccgacgc ccggcacgca gaggaaaaag
    10201 tacctgccgg ccagttcgcc aggcgcatat tccctcccca gcgtatcgag ccggatttcg
    10261 tacagccctt cggagatgtt gtcgcgcgac accagtgtcg acgccgtata tgacatgtcg
    10321 gtgattgagg ggaacgaagt cgcctcgccg ggggcgagtt cgcgctgcga ttcgtcctgc
    10381 aggcgctcga agtaggtgcg catcgccgcg ctgtccatgc ccgtcagggt gctgcctacg
    10441 ccaaacaggt ttgctcctgc tttgcggtat tgagcgatgt gctcagctgt cccgatgccg
    10501 cccatgccga tgacgattgg atctggtccg atggcgtccc ggacctgttg gaccgatcgc
    10561 aaacccagcg gacgaattcc gtcgccggac agtccgccga ctcggttgta cagaatcggc
    10621 ttttccccga cgtcagccat cgccgggcca atgctgttgg tgacggtgat cccggccgct
    10681 cccgcccgca ttgacgcttg tgccgtctcc ggcaggccgc gcatcatcgc cgcgagtttt
    10741 acgaacaccg gtaccggctt caccgcagcc accgccctcg tgatcgcggt caccagctcg
    10801 acatcctcgc cgatctgaag cccgtaaccc ttggcgtgcg ggcacgacat gttgagttcg
    10861 aaaccgtcgg cgatcgggct taaggtctcc gcggcggcga ggaattcctc caggcctccg
    10921 ccgaagatcg acaccagcag aaacttgttc gaggggatct caatgttgtc gaactctgcg
    10981 aggaatttct tcgcccccgg gttcgccagc ccgaccgcgt tgatataaca gccctcggcg
    11041 tagcgggcgt aaatcggctc gcggtagcct gcccgaggcg caaagctgat gctcttggtc
    11101 gtcacgacgc cgatcggcgc gacctccgcg aacgtcttgg cgatcgtcga cgcatgggtg
    11161 caccggattc ccgacggaat cacgaatcgc ccttggacct gcttgttccc tagcgaaaaa
    11221 gggcgagtga aaaagtcatt ctccactgaa ctgtgcactc ctgttccccg gcaaggtcat
    11281 tgtcgttgat tcccactcta attatcggac gtttcttcgt ccataccttc aggacggggg
    11341 ccgtaatggg gcggggcctg cccgaattcg taagcgccca gatcgggagc cttgccgccg
    11401 aacccgtcgt tcacattcgg cagccggacg ccggcatcga tggcggctga accgcctctc
    11461 agtcgcaggt ccgccgggtt gcgttccggc agcggcggat caggaaactc gacgctctcg
    11521 aacacgctca gatctacctg cacgccatgc acttctgtgg tcagccggcg catttcgtca
    11581 aggccgacga acgtcacccg cccgatctac ccctcgaacg gtgttccgtg cgtgcctacc
    11641 ccgtcatagt cgagatcgtt gagcggatcg gtcgccggag tgaagatggc tcgcccggtt
    11701 ccgcttccgt accggccgaa ctttccgcct cccttcccgc caacgcaaag attgttccgg
    11761 aagtacgaat tgccgtactg ggtcgggccg tgcggcattc ggaggccatc ccccactttg
    11821 accaccgtgt tgtggaggat tacgtcaccc aggctgcccc gcgaaagctt gaacggtgcg
    11881 tcgatgatgt tgtacatcgc attgcggata aagtagatcg ggccgcccag actaggttgc
    11941 gaactcaagc ccatgaagca gttcgtcaga cgattccgca tcactcggca gttccccatg
    12001 caaaaatcgg cctcgattcc gtcatcggcc ccgacgtaga tgtcgttgtt gtagatgtcc
    12061 accgacacct gctcgtgcgc gttccggtcc tccatcgttg aaatgcagtc gcggtagccc
    12121 ttgacgcggt tgtaacagat cacattgccg gggcctgtga tctggatgcc ttcgccgacg
    12181 cacccttcac caccaaagat actttcatcc cccatgccct ccggcgtcca gggcattacg
    12241 tacgtcacta tgttgtcggc gatatagcag ttcttgcaac cgggtggccg cgaggcggtg
    12301 atcccgtact tcgcgttgac ggtgcaatgg cgcaccgcaa cctcttcggc tccaaccagg
    12361 ctcacccggc cattcaccgt cagcttctcg agaatgatgt gcttgcggcc ttcgagagag
    12421 attccttcga atgtgcttcg gacaacgtgg ctggtgtccc cgcgaatcac gatcggcctg
    12481 cccggaaccc cgtcgcgcgt cgcggtgaac gattcgtagt agcccggcgt caactggatg
    12541 atgtcgccgg cttctgcgct cgcttcgacc tctcgaaagt tgttcggatt cgcctgcttc
    12601 actctcgcgc ccggcgtggg ccgcggcact ccgcgtgtgc gggcacggac cgtccgctcc
    12661 gtcgagcccc catccgggtc ggtcaggttg agccggatct cgtattcggt gttcggacgc
    12721 aggtcgaaga cgctacccga ttgcttgttt tcccacgaga aggtcggacg cgttcgattt
    12781 ccgctgtctc cagcgcggat ccgccggagt ggcattcctt cctgccatgc ctcgtcgcct
    12841 gcgcggcggt atttcacggt cacacgactg ttgaggttgt cgtccccctg gatctcccac
    12901 tcgatgctca agttccggat cgtggggtag ggcgtcgtca cgtcgcccgg cacgacgcga
    12961 tcctgaggca ccgtcggcag ggcggcacac acgaccagca gcaacgttgt tctcatatcg
    13021 gaaatcgcga tatctacagc ttcgccagtg tattgcgccg cgcccacacc ttctcgaagg
    13081 cctgtacgta ctgatcgagc agttcgggcg cttccccgta caggatgggc atgaagatgt
    13141 gagtcgcgtt cacttgggtc gtgccgacca gagccgccgg aatctccggc ttgtgatgcc
    13201 accacttcgc ctcggagtag atcttcatct tgtgctgttc cggatagtcc cacttgctgg
    13261 cccggacacc ttcggcttgc agcgccttga gcaacacatc gcgcttgaat cccgccttcg
    13321 cttcgtccag gaacagcatg ctggcgtagt agtagacccg cgtttggtcg ggccggcatc
    13381 ggggctccga caagccggga agttggagca aacggtcgtt cagcgcccgg atattcttcg
    13441 ccgctacggc gtttcgctca tccaggccgc gcaactgctg ccgcgccact gccgccgcga
    13501 acggatgcat ccggtatttc tgtccgaaac ccgttccctc gtactgccgc accgggctgt
    13561 cttgggggaa cttgggcggg tcttcgtagt ggccgaaggc ggcggccctt tcgaagtact
    13621 cgcgagtctg gtacattccc atgccgccct cgaccgacgg cagtaccttg gacgcctgga
    13681 aactgtaaat ccccatcaca ccccatgctc ccatcatctt gccttgcatc gacgcgccgt
    13741 gcgcgtgggc ggcgtcctcg agcaagatca gccctttctc ctttgcgaaa tcggagatcc
    13801 ggtccatctc acacgggagt ccccaggaat gcatcgtgca gatcgcccgt gtccgtttcg
    13861 tcagtttccg cttcgcgtct tccagatcga aacaggccgt gctcgggtcg atgtcgatga
    13921 aaatcggcac gtagccgaag aagcgcatcg ccaggcaggt cgcgaagaag gtgtaggagg
    13981 gcaccatgat ctcgctgccg ggcggaaggt cgagcgcgga atacatgctg gtgagcgcgc
    14041 tggtgccgtt catgtgagct ttcacgaacg gcgacttcgt gtattcctgc cactccttct
    14101 cgaaaatcgg gagctcttca tagaacttcc cgctgtcgat gaggtcgtgg agagctttct
    14161 tctcagccgg tccgtagcgc ggccacttcg tgagagccga ctgctgggag accgggaagg
    14221 taacggattt cgggcctcca tccagcgcca gcgcctccgg cgcccgcgcg gagagtgtgc
    14281 cgctggcggc gccaagaccc gcgagagccg caccgcccgc ctgaacgaat gaccggcggg
    14341 tctgccccgc gttcttctcc agcttctttt cggacatgga attctccttc gaatagagtc
    14401 tactcgatca acagcgcccg gatggccgcc ttgagccgtg cctccgtgcc cggtcctgcg
    14461 ttggaggcgc tgggctcgta cccgccctcc tggaacgcct ggtccgtgca caggtatccg
    14521 ggctggatgt cgccatagcc ggcgacggcc acgaaccgtc cggccgcctg tttgacggca
    14581 tagttctgaa actcgagcat cggttcgccg ggcagaaaca gcgccgcggc cttcccgatt
    14641 tcgagcagat tcacctccag cggacgcttc cgatcggcga acgccaccac cagcgccgcc
    14701 cgatacaatt gctcaccgga gtggggcgcg gattcccgca gcaccgtccg tgcggcctcc
    14761 gccggtttcg gcgtcaatac cagttccctg tgccgccacc gcatgttgcc cgccggcgag
    14821 aaccgcgtcg ccgcggcgga agcggccatg ccctgcttca acctcccggc cagttgctcc
    14881 cgcgattccg gcgtcccgtc gttgtacttc cccacggtca cgtcgcccga acagcccgtg
    14941 aaatagatct ggaacacgcc ttcttcccgt tccatcgcct cgcgggctgc tccagcgaaa
    15001 tcgcccgtaa cccgtccatc gcaacagaac gtctggggat gcgtcgcata gaagtgaatt
    15061 cgtgcgagcg gctttccgcg gcgcgccagc gtgatcgttc ggagcttcgg gtcgatcggt
    15121 ccttcgggca acgcggcgag cttcgggtcc ttgccgcccc cgctgaagcg tgtgatgagt
    15181 ttgccatcgt ctccgaatag gcggcgggcg gacgccacgc gatccacggc gctctccgaa
    15241 atgccggtcg aatcgaacgg ttctagtctc ctggtcgcct cggccgcggc tcgggcaatc
    15301 ctgtctgtca ggtcctcgag gtaccggccc gtcatcaatc ccgccggccg tccgaggcgc
    15361 gacaggatct cgttggagtc gccgtccacg tacggcgccg tgtgctggtg cacaacatgg
    15421 accgccacgt tcgtgacgtc agtctttacc gccgcgctga tcttccgccg gaaaagctcg
    15481 tggatcgagc tgcctatccc gcaccagtcg accgtgcaca ggacgtaccg ccggttccca
    15541 tcggcaagaa caagtccctt tgtccagagc gggtcttcca ccttcgcggc aggcgtgagc
    15601 cagatcaggg gctctccatt cggcggcgtg gcgtcggcgc gaaacgtcgc cacccgaagc
    15661 tcgccgcaaa gtggagccgc cgccaccacg gcagccgcca cccatttcaa aagacgccgc
    15721 aaacggagtg agcctttcga gggcatcctc acctcaaccg tacatgagtg tatcctgttt
    15781 gaggcatcac taaggagaac cggacatgat cccgtactcg gaacagcgcc gtactttcat
    15841 gaagtgggtt gtggctgcgc ccgtcttcgg aacgatcgca gcgaaaggat tgtcggagaa
    15901 gctgatggcg gccgccggca aagcctccag cagccatgtc tacacgcgcc tcggtatccg
    15961 gcccctgatc aacgcccgcg gcacctggac ttacctcagc ggctcgctac tcctgccgga
    16021 ggtgcgaaag gccatggacg acgcagcgcg ccattttgtg gacatctacg aactcgagcg
    16081 cgcagccggc aagcggttgg ccgagctcgc cggcacggag ttcggaatgg tcacatccgg
    16141 agccgcggga gcgatggcgg ctgcgaccgc cgcctgcatt gctgggacgg accagaataa
    16201 tgtgtggcaa ctgcccgata ccacaggtct taagcacgag gtcgtcatgc ttggaggccg
    16261 cagcgctttt gacagcgcga tccgccttgc cggggggaaa cttgttctcg ttcactctct
    16321 cgacgaactt gcccccgctc tgaacgaccg gaccgccatg gtctacacca cctggctcgg
    16381 cgaccgtctc gaaaaggcgc tcgcaatcac aaagaaggct ggcgtgccga tgctcctcga
    16441 tgacgccgcc ggcatcccgc ccattgaaaa cctgcgcacg tatgccaaga tgggcatcga
    16501 tctgttctgc ttcagcggcg gcaagggtct ccagggcccg caatgctccg gcctgctcct
    16561 cggccggaaa gacctgatcg atggcgcgct cgcgaattac agcccgtggg aaggcgccgt
    16621 ctgccgtccg atgaaggtcg gcaaggagga gatcatcggc gtgctcgccg ccgtcgaatc
    16681 gtggttcaag ctggacctca acaaactcaa tgcggaatgg gagaagcgcg ttcgacggat
    16741 cgcgaagctc gtggaaaccg ttcccggcgt caccaccgag atcaagattc ccgaaggtgg
    16801 caaccggtac cccacattga ccgtgaactg ggacgagcag gccttcggac taacggtgga
    16861 gcagtgcgac cagcaactcc gcgacggcga gccgcgcatc gaggtgctta cctcgaagaa
    16921 ccccagtctc gttcccgtgg tgagcgaggg cggcggcaaa agttcgaagt ccggtccccg
    16981 gccgaaccgt cttcaaatcg tctccttgac gattcaggaa ggcgaagatc tcctcatcgg
    17041 tcgccggctc cgagaaatcc tcggtgccgc gcgcaaacga accgcaagta gttgacgatt
    17101 acgccactac ctccacgccc agacccatga gggcgccgag ccgtcccatc gcctcggagt
    17161 agtctccata cacccacacg aggtgattgc catactcggt ctgcttgcgg acgaacgctt
    17221 cgccgttccc cgtctccgac ggtttgatga acgctgccac tgaacagccg aggttgtcct
    17281 tgtcccagcc ttccgactgg accagagtcc ctttcatcac cagtacctgc tttgcgttcg
    17341 gatgcatccg agcgacggta acccgtttct cgctgttctg catgaagtcg accaccgcct
    17401 tcgtgcccca gccggactga acaaatcgcc ccagcttgta cggcagaccc ggctgatcga
    17461 acccattcat ccggattcca ggagcgctgt gattgatctc caccacgccg cccttgcgga
    17521 agaacatgtt tccgagatgc gaggacttgc ccgacaccga catcagcagc cgcatcccta
    17581 gcagcgcgcc catgtcccct tcacacgacg acgcgatgcc ctggtccttg aacagcgtgt
    17641 ggatcaggca cggcgtgatc ttccatttct cgggaaggcg gctggcgcag aactcaaaac
    17701 actcgacggt gaacgcgttg caggcatgcg cctccatcaa tcgttcaatg gtccgcttga
    17761 agagaaggct ccggacgacg tactcccgat ccaggtaact ttggacggcg ttccggatca
    17821 acgcgtccgc ttcggcgccg gtcccctgtc gcacagcgtt gtccgcaagc gtcgtctcca
    17881 tggactcggc cagtgctttg tacgggagca ttctgacctt cacgcccaac ctcgcttcta
    17941 gatcctgaag gtccgtgatg cccgtcaagg aagccaccga cggaaagccg cggttcgtcg
    18001 ggaacagcac cgaagtctcg cggaagatct tgcgtgcccg cagcagatcg atcgtactcc
    18061 ggagttcctc gttgtcctcg accaccagca tctcgtcgcc ctgcgtcttg gcataggccg
    18121 ccacgtcgac cgtccggcag ttgattccga acaagatgcc gggttt
//
LOCUS       DATIFQ010000388        65767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_705327, whole genome shotgun sequence.
ACCESSION   DATIFQ010000388 DATIFQ010000000
VERSION     DATIFQ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 65767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65767
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705327"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VLE22_18795"
     CDS             complement(<1..526)
                     /locus_tag="VLE22_18795"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HSB16507.1"
                     /translation="MEPSKKKRGSTLLEFTLVGIPVIFMLISIFEISRGMWLYHTLAS
                     AIKDGTRFAIVHGNNCSIYPSNCTVTIRDISERIRRNAVGFVPDDLQNLEFISATRGT
                     FTCATLAACLDTGNPGDLSWPGGPVGSAPEDFPDGRQFAWVEIRAQYPFRSAIAMFWP
                     GAGRGQNFGEFLLPA"
     gene            complement(529..996)
                     /locus_tag="VLE22_18800"
     CDS             complement(529..996)
                     /locus_tag="VLE22_18800"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HSB16508.1"
                     /translation="MKKPCKEARRGHAIVELSLMAPWIFFLFVGALDMGFYAYALITV
                     QNAARVAAITTARSEGTAGNREYACELVLREMERLPNVPDPASAYTDLSCSDLPASVQ
                     VDASSGLDADGKLFSRVQVRYETVQLIPIPGLLTGKTTISRTAESRMYGRTRP"
     gene            1195..3489
                     /locus_tag="VLE22_18805"
     CDS             1195..3489
                     /locus_tag="VLE22_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16509.1"
                     /translation="MMMIWSLALLVQTATAPNALPGTSPLLEEADYAVRMVEGIHRFL
                     DRETAAAAAARESRWKRAFRTRNAYEASVAPNRERFRRIIGLADARVPYDAPRLDVVF
                     GGAVIVAKGSGYTARAVRWPVLPGVDGEGLLLEPDREPVARVVALPDADWTPEAIAGM
                     SPGLPPEAQYARRLAEQGVLVLVPVLIDRKDNFSGNAALGCLTNQPHREFIYRMAYEV
                     GRHIIGYEVQKVLAGVDWFSRSEKKTPIGVFGYGEGGLIAFYSAAADTRIDAAGVSGY
                     FGPREGLANEPIYRNVWGLLREFGDAEIAGLIAPRALVVETAPGPVINGPRLETKERK
                     GAAPGALVGPDPRQAGKEIHRVLRWHMQLPGTPRPLFSFSLGGTSRISEHGTLNAFLE
                     ALGAKYDLNLHGAPPGDLRTGFVPEARQRRQFNQLVDFTQAVVRGAEAARRKFWAKAD
                     ASSVEAWQRSTEWYRRYLWEEVIGRMPDPSEPLEARTRGIYERPNWTGYEVVLPVWPD
                     VIAYGVLLVPKEMKPGERRPVVVCQHGLEGRPQDLVEPKTPAAENAYRRFAARLADRG
                     FIVYAPQNPYIFETQFRQIQRKANPLKLSLFSFIIGQQQRALSWLSSLPFVDPQRIGF
                     YGLSYGGKTAMRVPPLLERYALSICSGDFNEWIWKNTSVDQPFSYMFTQEYEMPEFDL
                     GNTFNYAELASLMAPRPFMVERGHRDGVGIDEWVAYEYAKVRRFYTFLKIPDRTEIEY
                     FDGPHTIHGVGTFRFLEKHLRWPE"
     gene            complement(3494..6013)
                     /locus_tag="VLE22_18810"
     CDS             complement(3494..6013)
                     /locus_tag="VLE22_18810"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HSB16510.1"
                     /translation="MIRNALPLLAAAALSVWARPNPYPAVRQAGNYMHNYYIPPAPGS
                     TPWAPCWSPDGKWIAVAMQGSIWKVDPRTGGAEELTYGTTYHSSPAWSPDGRWIVYTA
                     DDDGAKIQLEILDLKSGRTRALTSDSQVYLDPIFSPDGMRLAYVSSKPNGHFNIFVRP
                     IRDGDWAGEEIAVTRDNQYPRDRLYFGPWDMHTQPAWAPDGKEIVFVSNRGVPLGAGD
                     LWRMPAEPDGILKAKRILAEQTLYRTRPHVSIDGKRILYSSTGGAADQFDNLYVIPIE
                     GGAPYELTFGEFDRFHPRWSPDGEWIAYISNDGGLPQLALLETYGGANRVIRITSRKW
                     KRPMGTLRVRVIDAGTDKPTPARVHLNAADGKFYTPTDVYSRIGASGRHLFHTAGAFT
                     VELPAGTVTVEALKGFEFIPALAKTEVPAGGDAELTLTLRRLADFSAKGWHNGSMHVH
                     MNYGGNLRNTPENLLLMSDAEDQDFLNAQAANKDNRVLDHQYFAPGGGEHPASRGRSG
                     SKLIFGQEYRPPFHGHTSLFGLRDHLMSPFTTGYEGTAIESLYPTNTDIFRKARAQGA
                     VTTYVHPYGDADPLPAGLGNAKAFPVDAALGTVDALEWSDAGRGGLIVWHHVLNNDLP
                     VAPVGGEDSITDLHRSKLIGSARAYAWLGSDVSAEAWLDAIRGGRTFFSTGPLLEFRV
                     DGKIPGETVRLPAEGGTISIEASAVCIAPLSKVEIHHNGKVLMVVPLAADKKSASLKT
                     QLKLTGSGWLSLYAEGPAHPYFDATFLQAATNAVRVYVGGQKIRSRESAEYFVQWIDK
                     LSRMTADWLWWRSEQEKTHVLGQFGEARAVYERLASEAGEH"
     gene            complement(6027..6986)
                     /locus_tag="VLE22_18815"
     CDS             complement(6027..6986)
                     /locus_tag="VLE22_18815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB16511.1"
                     /translation="MKRRVFVGGAFAGLVAPAARSQKLKAGDIPKRVFGKTGEKLTVI
                     GQAGGRLDLAPLDEAKAVVRRAYDLGVNYFDNARAYGKGRSEEVYGAVLPEFRKNVFI
                     TTKSVQRTREGAEADLVLSLKAMKTDYLDLWQIHSVSEMEDVDRIFAPGGAIEAFEAA
                     KKAGKCRFIGFTGHHDPHVHLEMLKRYDKYDTILMPLHAADPSYLSFEQLVLPLAVER
                     GLGIQGMKNFGNAKLLSEFSVRECLRYVLSLPVHCTAIGCTTIGQIEDDVRVAREFQP
                     LTASEMDAMRKRGQPIAGPALEDWKRNVQARAGAAPLLEYRGG"
     gene            complement(7051..8160)
                     /locus_tag="VLE22_18820"
     CDS             complement(7051..8160)
                     /locus_tag="VLE22_18820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB16512.1"
                     /translation="MATATQFETEQTAARRDAFIERLFQSALGAFDIFTIHLGDRLGF
                     YEVLSADGPLTSAELAARTGTHERYAREWLEQQAMAGILDVKETTGGNGARRFCLPSG
                     HEEVLAQRDSLNYLAPLAQIVAGAVRPIDALLTAFRTGGGVPFAAYGADVREGQARIN
                     RAAFLYQLGKEWLPAIPDVHSRMLGVSPPRVADVGCGAGWSSIGIALHYPNVRVDGFD
                     LDGPSIELARANAGQYAIGERVRFFVRDAGDPGLAGQYDLVTAFECIHDMSNPVGALR
                     AMRRLAKDDGTVLVVDERVGEEFQPGASVVERLMYGFSVLHCLPSGMADQPSAATGAA
                     MRPSTLRRYAMEAGFRDVEILPIDNFLFRFYRLLP"
     gene            8297..8881
                     /locus_tag="VLE22_18825"
     CDS             8297..8881
                     /locus_tag="VLE22_18825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB16513.1"
                     /translation="MSEVSEGTGPADVTQLLGELRSGSRSAAERLFPLVYEELRRLAR
                     GQLRREHGDHTLQPTALVHEVYLKLAGSAPERWRDRAHFLGIAARAMRQVLVDHARRR
                     MAEKRGGQWERTTLAEGRYGIAATLEDLVALDAALDRLDAVDPRLRQVVEHRYFAGLT
                     DREIAEVMGITERTVQRDWARARAWLYKELYPAP"
     gene            8888..12340
                     /locus_tag="VLE22_18830"
     CDS             8888..12340
                     /locus_tag="VLE22_18830"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB16514.1"
                     /translation="MKTADRWKKITALFDGALEQPEARRAEWLATASDGDEDARAEVE
                     RMLAAHGIAGGILDHPTFTMSPTDLQEDDGPQVGSAVAGRYRILREVGRGGMGVVYEA
                     HDPRLDRQIALKFLPPRAGTEAKARFLTEAKTASAIDHPNICTVYDAGDTDDGRLWIA
                     MAFYDGETVAQRLRKGPFSVDKSIRVASQVLRGLECAHGAGIVHRDIKPSNVMLTARG
                     EVKILDFGVAKLAHESPETRPGTRVGTAAYMSPEQARGEVVDHRTDLWSLGVVICEML
                     SGQRLFRGEHEVSVMHAILYEEPPTPSLPSGLDRIVRKALARQRADRYAGAAAMLADL
                     ERFLDPGVSPAVRLPAPLTSFIGREREIAQIDGLLSTARLVTLTGPGGAGKTRLALEA
                     ATQLAVKFEDSVVFVPLAAINDPDLIAPAMAQVVCSQRSPGLGPIEELRFALRDRKTL
                     LVLDNFEQVAAAAPVVAELLAACPGVKMIVTSRVPLRLTCEHEFPVPPLPLPRRGDPP
                     DPRTLERNPAVALFVDRARAARPDFALTAENAADIAELCARLDGLPLAIELAAARVKM
                     FPPHAMLERLCHRLDFLKTDQKDRPARHQTIRQAISYSYDLLSEHEQRLFRRLAVFVN
                     GMTLDAAAAVANAGGPLELNVEEGISGLVDHSLLRRSDDAGTEPRFRMLEIVRDYALE
                     RLEESEREYTSEAHALHFLALAERAEPLLTGPIQVEWMDRLEAEHDNLRAAIAWAEEK
                     GEAEAGLRVGAALWRFWLVRGHLNEGRRRLDQLLALPGKTAPLVRARALNGMGTLAHN
                     NGENTEARVFLEEALTLFRQAGDRRGVASVLGNLGWVACELTDLETAEILSTEALELN
                     RDLGEKRGYALALNNLGWVANYRSEYRRAVAYQEQSLALRQEIGDSRGVAFTLSNISW
                     AEMCLGDYVRAETLADRALEMLRELHDRSILGWALVIRGWIASEKGEHERAAAFVEEC
                     YRLAREAGNRSIRAAGLTGLGMLATVRGEYDRAVSLFEEAISLWQAIGGRWGVALSLH
                     GRASALHGTGDTRRALSDLRTSLRMRSEIGDRRGIAGCLEGIAGIEAEQHRFRNSARL
                     LGGAAKLRETAVTPVPPYLSSELEKVAAKARGGLGVREFGAALTEGREAPIDALVRSC
                     LEEA"
     gene            12362..13195
                     /locus_tag="VLE22_18835"
     CDS             12362..13195
                     /locus_tag="VLE22_18835"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB16515.1"
                     /translation="MLIIDTHAHIYSPDEKKYPPIEKPLRPPRGKASVEDLRKQTQSA
                     GVRGACLIQTSTFYSFDNRYTCDSAKANPDWTAGVCTLDPDNPKSPDILRQFVRDFGM
                     KGMRSIPATDGRLNHPSVEALWKTALDLGIVINILIGRNKADEADAMLGKFPKLRVVL
                     DHCLNPQAGPELDATLMAVLRLERRKNLHAKLTFVPTGSKEGYPCRDMHDACLKVLRA
                     YGAERCVWGSDFPNDLWTPKVSFEEHLKIFTHDLPLKPAERSAVLGGTANRLWFGGKL
                     G"
     gene            13192..15156
                     /locus_tag="VLE22_18840"
     CDS             13192..15156
                     /locus_tag="VLE22_18840"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HSB16516.1"
                     /translation="MRLQVGLAALLVSVPLFGDPSLPALFSDHMVMQRGVPAPVWGWA
                     DPGERITVTIGVESRAAVANAQGKWEVRLSPLTTGSPLTMTVRGNRTLTVQDILAGEV
                     WVCSGQSNMHFGMLATTTAKEDIAAANHPEIRLFTVPRESALEPLKDFRSAWKVCTPE
                     SVAEFTAVGYHFGRNLHERLNVPVGLIQSSWPGTQAEEWTARKALDADPDFTPILDRW
                     NQAGPEIHNLYRRYRRPADFDLTFDDFEFVPFEGGSGTQPLDDFDGGAGNRFGGEWTN
                     RSRPEDKFAMDRVRPGYSGNGEALRFSGPQRAPDTVQIHSAFGAAGESRDMSKFSGLR
                     FFVRGKGFFRIHVLQQSVRDSDNYTSAILGASGDWTPVTVRFSELKRGGWGRPASFAP
                     EELTGILIEAIPAAITPPRPPSGLFNAMIAPLAPFAIRGAAWYQGEGNAGRAYQYRKL
                     LPAMIHSWREAWGQGDFPFLIVQLPNFRQRKAEPGEGNWAELREAQFLTYKTVPNTGL
                     AVTIELGEADDVHPRDKREVGRRLALAALGAVYRQERVYSGPIQDSVKVEGSRVRVRF
                     RNTGGGLIAKDGGPLKGFALAGEDRKFVWAQAKIEGHSVVVWSDSVPRPAAVRYAWAD
                     NPECNLYNSDGLPAPPFRTDDWPGLTREAR"
     gene            complement(15169..16806)
                     /locus_tag="VLE22_18845"
     CDS             complement(15169..16806)
                     /locus_tag="VLE22_18845"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16517.1"
                     /translation="MRSRFAISLLFATGLLLTACRKETTPKAEAMPAKQRLSKYVSFR
                     LETDLSSLTENERRMIPLLIEAARVMDDLFWLQAYGDKNTLLSGLAGPDVRRFAEINY
                     GPWDRLDDEAPFVSGVGPKPLGANLYPADMTKQEFEAAVAVAPDGGKQLKSLYTLVRR
                     KADRSLEARPYHTEYGTQLQVAAAKLREAAKLASSPGLRKYLELRADALLTDDYMASD
                     LAWMDMKDNTVDIVIGPIETYEDRLFGYKAAYEAYVLVKDKEWSRRLAKYTSMLPALQ
                     KGLPVPDAYKQEKPGLDSDLNAYDAIYYAGHCNAGSKTIAINLPNDEEVQLKKGTRRL
                     QLKNAMRAKFDKILLPIAGVLIAADQRKHVGFDPFFAETMFHEVAHGLGIKKTIDGKG
                     MVREVMKEHASSLEEGKADILGLYMISELHKKEELGKAELMDYYVTFLAGIFRSIRFG
                     GTSAHGAANLVRFNYFERAGAFTRGAASGTYRVNEEKMQAAVDSLSEKIIRLQGDGDY
                     DGVGRFFAEMGTSSDTLKSDLMRLDGAGIPVDVVFEQ"
     gene            16867..18150
                     /locus_tag="VLE22_18850"
     CDS             16867..18150
                     /locus_tag="VLE22_18850"
                     /inference="COORDINATES: protein motif:HMM:NF027805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HSB16518.1"
                     /translation="MNEIKLITLNPGHFHAALVQKEMYPDVSRQAAVYGPLGPDLLEH
                     LGRVVRFNVREESPTAWELDVRCSPRYLDRMLADRPGNVVVIAGRNRAKIDLIHASVQ
                     AGFHVLADKPWIIETADLPKLEETLETAERKGLAAYDIMTERYEITSIVQRALVNDDA
                     VFGEMVEGSEDAPAVLMESVHHLMKTVAGVPLRRPPWFFDIREQGEGLTDVGPHLVDL
                     VQWVLFPEQAIDHRIGVRVLNGRRWPTVLSLEEYQRVTGERRFPDVPEASGAKIEYFC
                     NNSVLYRLRGVHVKLTAVWNYEAPPGAGDTHCALFRGTKARVEIRQGAEEGYRPELYV
                     VPEQPGEAVGSALRSRVDSLASEYAGIALEHQGARYHIVVPEVHRVGHEAHFAQVARQ
                     FFRFVRGEGGLPACEKPNMLTKYFVTTQGVALSRL"
     gene            18250..20898
                     /gene="clpB"
                     /locus_tag="VLE22_18855"
     CDS             18250..20898
                     /gene="clpB"
                     /locus_tag="VLE22_18855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HSB16519.1"
                     /translation="MDINRFTEKAQQALQAAQSKAVRFSHQQIDVEHLLAALLEQEGG
                     LAPAILNKADVRTEGLRQRLEQELDRLPKVSGSSGTPDQIYVTGRLNRVLTQAEDEAR
                     RLKDEYVSIEHILLAMTDDGGVTGRLFKEFGLTRDRLMKALQEVRGNQRVTSQTPEGT
                     YQALERYGRDLTALAGQGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAI
                     VEGLAQRIVRGDVPEGLKNKRVVALDMGALIAGAKYRGEFEERLKAVLKEVQSSEGEI
                     VLFIDELHNVVGAGKAEGAMDAANLLKPMLARGELHCIGATTLDEYRKHIEKDAALER
                     RFQPVLVDQPSVEDTISILRGLKERYEVHHGVRIKDTALVAAAVLSNRYITDRFLPDK
                     AIDLVDEAAARLRTEIDSMPAELDERLRRIMQLEIEREALKKETDAASRERLARIEKE
                     LADLKSEADALKAQWQAEKDAVQRLQSLRGQLEQTRHEIELAERQYDLNKAAELKYGK
                     LTGLERQIKQEQAALEDKQGQMQLIKEEVDEQDIAEVVSRWTHIPVSRLLESEIQKLL
                     HLDEELHKRVIGQDEAVQAVSEAVLRARSGLKDPNRPIGSFIFLGPTGVGKTELARAL
                     AEFLFDDERAMIRIDMSEYQEKHTVARLIGAPPGYVGYEEGGQLTEAVRRRPYSVILF
                     DEIEKAHHDVFNVMLQILDDGRLTDGQGRTVDFKNTIVTMTSNVGSHRILGYRGAFEG
                     TEYERMKEAVLDEMRRSFRPEFLNRVDEVIVFHALSEEHLAAIVSIQLERLKPRLAER
                     HLELEVTDAARRHLVRAGYDPNYGARPLKRAIQKEIETPLARLILKGDVREGQTVVAD
                     LDTERGELKFTPRTVPSGEKKSAGAS"
     gene            20902..21264
                     /locus_tag="VLE22_18860"
     CDS             20902..21264
                     /locus_tag="VLE22_18860"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSB16520.1"
                     /translation="MSKKPRHPSSFMLPIAGPNSDPVWQPVADVYRTLTGWLVKFELA
                     GVDPGDVEISVENTRLTVTGMRRDWTFEEVCSHYSMEIAYNRFERSLELPCNLERAHL
                     DLEYRNGILLVRVTTKGE"
     gene            21268..23619
                     /gene="lon"
                     /locus_tag="VLE22_18865"
     CDS             21268..23619
                     /gene="lon"
                     /locus_tag="VLE22_18865"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HSB16521.1"
                     /translation="MHDHTKVLPLLPLKNSVLFPHLLMPLSVGRPTSLAAVDAALSTE
                     EKEIVIAAQRDAAVEVPGMADLYSVGTRAVIKRMSRPSESAMELIVLGTERVMLLRLD
                     STDPYLKVEVQPLALPAEESPEVEALHHEVMELAAKAIGLAQPQAPAEMSRVMMSTED
                     PLRLCYLLASMFSLEIDKEQALLEAATRADALRLTHSFLAHEIQVLEIRNKIASTAQS
                     EMGKEQREYLLRQQLRAIQQELGEKNPEQAEVDMLKERLEKADLPDEIRKETERELSR
                     LEKLPSASPEHHVIRTYLEFVLELPWRVHTDDQLDIAQARKVLDEDHYNLKEVKERIL
                     EQLGVLKLNPQAKAPILCFVGPPGTGKTSLGQSIARAIGRKFERMSLGGMHDEAELRG
                     HRRTYIGAMPGRLIQAIRRAGANNPVLMLDEVDKLGRDFRGDPAAALLEVLDPEQNKT
                     FRDNYLDLPFDLSKVLFITTANTLDTIPRPLLDRMEMLRLPGYSEEEKLIIARRYLIP
                     RQLKETGLTAEQCVIGDDVVSRIITGYTREAGLRQLERGIGRVARKVALRFAEGPSEP
                     VTVHPDELADMLGPERFFPEKMRRDLAPGVATGLAWTETGGDVLYVEATLLPESKGLT
                     LTGQLGDVMQESAKTAQSYIWSHAEALGIDRALLKDAGVHIHVPAGAIPKDGPSAGIT
                     MAVTLASLYTKQPVRSDTAMTGEVTLTGLVLPIGGLKEKVLAARRAGIKRVIVPKPNQ
                     TDLRELPEEVRSEIEFIFVERVEEVISAAVPALAERLSPVKVG"
     gene            complement(23659..24828)
                     /locus_tag="VLE22_18870"
     CDS             complement(23659..24828)
                     /locus_tag="VLE22_18870"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HSB16522.1"
                     /translation="MAKLSIRDLDLKGKRVFIRVDFNVPLAPGGQEISSDKRIKASLP
                     SIKYALEQGAGLILASHLGRPKGKPNPEMSLKPVAKRLEELLGRPVKMAPDCVGPEVE
                     AMKPAPGEVLLLENLRFHSEEEKNEPEFARKLASLCDVYVNDAFGSAHRAHASTEGMT
                     KFVSKAAAGLLMDKELEWLTKATKNPERPCIAILGGAKVSDKIEVIQNLMKFVDRLLI
                     GGAMAYTFLKAQGQPTGKSLGEAEKVDLAKELLAAAGGKLLLPLDHVVAAELKEGAAN
                     EVVETIPDDKMGLDIGPKTINAYAKEIAASKTVIWNGPMGVFEKPPFDKGTMALAKAV
                     AESGAVSVVGGGDSEKAIKSAGVADKITHISTGGGASLEFLSGIDLPGVAALTEK"
     gene            complement(24838..26151)
                     /locus_tag="VLE22_18875"
     CDS             complement(24838..26151)
                     /locus_tag="VLE22_18875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSB16523.1"
                     /translation="MKLLGMSLVACSLFAAEPDWAKLEPYALDLLQRYIRIESINPPA
                     NTAEAANLLKTELEKHGIDVKLYTSGPGGQTNLLARLPGKDRSKKPLLLLNHLDVVPV
                     DRSAWNIDPFGGIAKDGWIWGRGAMDMKGIGIQHLTALVALKQSGIVPARDIVMLSTA
                     DEETGGTYGILWMIKNHFDEINAEYVLDEGGFGTRDILAENKLVFGIAVGEKQTTWLR
                     LRAKGTAAHGSQPIPDNANMTLLRALERAMERPPAKPHPVVEQMIRNIGAALAKNKYT
                     NAIQDNTISLTTLSAGVGSPPKINVIPSTSEATLDCRLLPGVDAEEFISEMKARINDP
                     RVTIELASEPNDPGVSPSDTPLFAAIRESIAKHHPGAIVTPMLVPHGTDSVKLRVKGI
                     TAYGFTPMVIDMATSATMHSDAERIPLSEFYKGIRIIYDVLRSDI"
     gene            complement(26148..27101)
                     /locus_tag="VLE22_18880"
     CDS             complement(26148..27101)
                     /locus_tag="VLE22_18880"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HSB16524.1"
                     /translation="MASYFLGVDGGQSSTTAMVGDENGRVLGLGRGGPCNHVGAAEGR
                     AKFTRVIRECVGKACAQAGLEQSSFRFAAACLGFSGGPADKQAILEEILPTERLAVTT
                     DALIALSGATAGEPGIITIAGTGSIAFGRNTAGKTARAGGWGYVFGDEGGAFDVVRQS
                     LRAALRYEEGWGPPTVLRRVLLEATGAPDANDLLHRFYTTEYSRPHIASYAALVDQAA
                     VEGDAVAGRLLENAAQELASIAAAVRGQLFDRNESARVSYIGGVFRSGILLERFRMLV
                     ELEEGNRCGPPVYGPAAGALIEAYRNAGLAPKITNLPEAEK"
     gene            complement(27154..27882)
                     /locus_tag="VLE22_18885"
     CDS             complement(27154..27882)
                     /locus_tag="VLE22_18885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB16525.1"
                     /translation="MAISIRDKVAVVMGASSGIGRATALALARYGARVMAAARREDRL
                     AGLPEEARREGGVLDTCPCDVVDRAQVEALIAATLKRFGQLDILVFATGTNIPARSMK
                     LLTAETWDMMIATNLTGAFHCTHAVLPVMRDAGGGLIIYISTISAHVPDVSGAAYQAS
                     KCGISGLAHATRVEEKANGIRTSVIFPGLCLTEILDKRPSPTPKEMLDLALEPEDVAD
                     AVVAVARLHPRAVVPEMELVPSRL"
     gene            complement(27942..28925)
                     /locus_tag="VLE22_18890"
     CDS             complement(27942..28925)
                     /locus_tag="VLE22_18890"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB16526.1"
                     /translation="MAALSEAQPLELVDLRKISAADLSHLLEHELREWRRGLDWDFRP
                     SAELVRRFVGMQALSGYALLAGRRVAGYSYFVTEERKGLVGDLYVLHEHRTPRNENAL
                     LEAVLNDLLAIPYLRRVEAQLMMMVVPNGRNLPKGEFLSVHPRNFMVFDLCRTSELSP
                     GAAAARLHFETWSDGRQEEAGRVIAEAYSGHVDSGINDQYRSAGGARRFLTNIVQYPG
                     CGQFFQPASLLASDVSTGRLCGVCLASLLARDVGHITQICVSPPMQGIGAGYELLRRS
                     LAVLAASGCRKASLTVTAANTGAVRLYERVGFATIRQFPALVWEGFAKDIW"
     gene            complement(28978..30159)
                     /locus_tag="VLE22_18895"
     CDS             complement(28978..30159)
                     /locus_tag="VLE22_18895"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HSB16527.1"
                     /translation="MMTRKALSAVSLVACVSAITLHAAEPARPGSTVRLCTAQPPSRL
                     IDYKRSPSEALAEVDRTLAELEKTVHRAGTAGCDAFALPEDTLGLVRWEAGNKKVLRE
                     VLPAAVARMLDRLGRAAAAHRMYLVCSSDTVEPDGKYRNMAFFLGRDAKEIGRYQKVH
                     PAISETDHERGTSFPVFETPDLGGVGMLICYDMVMPESSRSLAMAGADVIFLVTLGGA
                     TTAGDPDSDDESLNRASFRTRAADNFVYIVVAKRGGGAMVISPQGKVLAQGKAPGDIV
                     TADVNPFAGRRAGDAWNVQTDMRARLFRERTPAAYGILTDPAPPALKKVPETITPSEA
                     LRIAEGAMTVGSDRFSKAEALLRAGRTEEAIRAFEKLRADYPTTWIDRAAGAHLAKLK
                     R"
     gene            30238..32640
                     /locus_tag="VLE22_18900"
     CDS             30238..32640
                     /locus_tag="VLE22_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16528.1"
                     /translation="MSWRTGIVLLAAVPLLAGLRPHPRVEVDAELIAKVRELRKAGDL
                     AWARFDRWTNTSPEKDSDTNRIVACMFHYLVSNEEGKFNCAWQPVQSKVYRNGTDRSG
                     GLMPLLDLYNGDKHTAAYQGGVLVAIIARVYDWGYGRLSPEQRQDVVEWLNDAVAFLH
                     LNNDDGLLYLRNDGASVTQGVAAATYATLGENPKGEQLMAWFRERWRETLKGLDIMGK
                     GGATGEGNAYGTSPTGSGIITAANVAYTAAGEDLFLSHVWFRQRLLYDAFAAYPGTLG
                     GPGMPTGVPREPMIEQASIAGDGRRAVSWHNRDTRRNGLVLARRFAGTEEADTWNWVF
                     RQPEVDHPTDDSQAVYELVYYSPRPRLVKPKRLSFFDPSMGFVYIRSDWDSPDATWIS
                     FWAGPHIDTHQHLDQGTFTVFKRRDLAPKTGHYDSDVFYPHHLAYYIRTVSSNGILVG
                     DPKEIFRGFIAGMGCDENGNTLRTMTKATWPACIPNDGGQRTMAPQSLAVANAAQFER
                     YRDIFDVARVVSFKDDGRAVFVVADITNAYNNPRHTTPGNRPKVNRVYRRVVYIRGLD
                     VLAVADTVESTEAKFEKKWLLHALERIEIGGEVQKVGDGETVHQETDEAKIVVDDTSP
                     SDREQTTFDLRRGYAALNVKTLFPVKFRYRKVGGREPAETPHADLYTPGRNARHLHRH
                     VKDFWVKDFNEGVIPNHKSVNWAPERPLEAAQSEHVPVFGPGYGRWRLEVEPDAPGKT
                     DYFLNILKPALNPTEKMPNTRRIESDSTFGAEIVKDGTKYTLVFSKDGLEAPKLEIQG
                     GN"
     gene            complement(32658..33932)
                     /locus_tag="VLE22_18905"
     CDS             complement(32658..33932)
                     /locus_tag="VLE22_18905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HSB16529.1"
                     /translation="MTPPLFREAYELINEFKGDAYVHGFGVLPETGRLAASLGRRAVL
                     VRDAFAGAAGHERTILDSLRAAGVEVAGVLDGAAPNAPREDLARITEGLVALDPDVIV
                     SFGGGSTIDAAKAAEVLRALGGSIDDYFGTGLVSKTLVASGRRLTPHVAVQTVSSSSA
                     HLTKYSNITDIATGQKKLIVDEAIVPSRALFDYAVTTGMPRALTTDGGLDGIAHSLEV
                     LFSAVGKPNYGLLERVARAGISLVLEYLPKVLTHSEDREGREALGLATDLGGYAIMVG
                     GTNGAHLTSFSLVDILSHGRACGMLNPYYAVFFAPAVEEPLKLVAGICRDLGYAALGV
                     ENLEGRVLGEAVANALIGFERKIGVPTTLSEIPGFTDAHVERALTAAKNPQLRMKLEN
                     MPVPLTPEMVDAYMGPVLQAAKTGDLALIRNV"
     gene            complement(33934..34563)
                     /locus_tag="VLE22_18910"
     CDS             complement(33934..34563)
                     /locus_tag="VLE22_18910"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB16530.1"
                     /translation="MRFILLLLLAAAAFAVEGFVPLFNGKDLDGWFVMYKQGPGFVVE
                     DGVLVCPADGGEKLETEKEYANFVFRFEFRLEPDSNNGIGIRAPREGHTATQGMEIQI
                     LDQSGPKYGPMKLRPEQYHGSVYDVIPARTGFLKKPGAWQEEEIEANGRRIKVTLNGV
                     VILDADLDMVREPAVLAKHPGLARPSGHIILIGHGSRVEFRNLRIKELP"
     gene            34618..36744
                     /locus_tag="VLE22_18915"
     CDS             34618..36744
                     /locus_tag="VLE22_18915"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HSB16531.1"
                     /translation="MRLLICLLLMMAPAMRAGVDWVRTARVFLIDGYQPPFAPELEFD
                     AEALAKTMTEMNANVVRMSTMGKYAHIQGVRFTPHRALGKRDLLREMIAAAKPRGIRV
                     IPYISTGHKLAWSMVTKEYPEYGHRPRPGGQPDRSHMFVGEDHGTVCWNTPYRKAFMD
                     MVEHIVRDYEIDGVYFDTWRPFYFWQGMRLCYCDGCRNGFRAASGKEIPYHEKRSGYT
                     KAELETVDRYHAWYRERFIGIFGEVCRLVKSYKDIPLIYNINHARQIAEEDPRIIGAM
                     DVFLYERGNSLLERAEGVSLARAAGIGVWPYIGVYNNWPRVIYNNVDYQQEIYTTAMF
                     GGAPIIAQPWGYVDHAENRKFVSDPFFALKAHEKETEGFENEPYVAVVYGHKDPPGYA
                     RSDWFWKTDARTNTLGAFAACLHGHVQVSSVPEQIMDEPEKLGRYKVVYLAGTPHVSA
                     RSAANLMRFVENGGGLVVSYGTSLYGAEGVRQTGFGIEELIRVKPAAREGKLAEILAS
                     YQSMLGGPNDLYLIARTGAMAGKLIPLTYFEPVEVLDGGDVAMDIVTGDGRRRVLPGV
                     VLSRHGKGKVVYGASSLEGLYVQESLPVLRDYVNTLIRMAAPEAPPFETDAPPTLVFN
                     LTRKNSRRALHITNWSVSSERGGGSLAPAENVRVRLRVPEGKKVADAEVFGESGHAGA
                     VKGTSRVVELNFPRLEAYRGAVVEFR"
     gene            36800..38179
                     /locus_tag="VLE22_18920"
     CDS             36800..38179
                     /locus_tag="VLE22_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16532.1"
                     /translation="MSLGGAGLLSGLRAGDRVRPAGPGAKCVPVIKAAFVRRKEDYGM
                     LWPGAIYDGATALANYRRQIVAASEELGIRIDLRPEPVYSLEAADAWVAEARAQKADG
                     LFVALLDRQQHAWPSAEKAITSGIPSVIFSPIGAAFTTNTGPLATKNGGFICSTDDFR
                     QAAYGLKMLKAGAKLREMRFIVLAGNERRDEKVADFGTKLRYLPARTFLEEYNSTPVT
                     DEVRKIAAAYIQNATAVKGPTEEDVWNGVKSYVVARNILMREEGDGITMDCLGALGKT
                     KVSLPCISWSSMLDDGIPAACEADIGAAVTHALVQYLFDRPGFQQDPVPETAKDCLIG
                     AHCTCPTRLRGWSQPPEPYFLSYHHGKRDAVPVPRWNIGERITVADVLLRDRENPKPQ
                     MVISVGKVVENVSVPPAGGCVVSVMVELDGSPELLDYPGFHQLFFYGDFRRELHQYCR
                     LFGIQPILA"
     gene            complement(38204..39100)
                     /locus_tag="VLE22_18925"
     CDS             complement(38204..39100)
                     /locus_tag="VLE22_18925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSB16533.1"
                     /translation="MNSRRDFLKAAGAVSISAAAPQAAQPAERQKLPQVQLGCYSISR
                     LICGANVFNGGSHLSVFVNYEMKKYYTPEQILKTLRRCEEVGINCWQAGKGNLENYRH
                     YVDSGGKMHFLALEHSEPEVVKTLARGGCIGIAHHGEVTDRLFKQGKIDQIQDYLKMV
                     RDSGMLVGISTHMPAVVDTVESKGWDFDYYMTCVYERHRTKEELEKLLGQAPIPVGEV
                     YLPNDPPRMYRMVQQTKKPCLAFKILAAGRLSEHPQWIEQAFRNAFNGIKKTDAVIVG
                     MWDRYSDQPGENAEFVRRFGAA"
     gene            complement(39175..40785)
                     /locus_tag="VLE22_18930"
     CDS             complement(39175..40785)
                     /locus_tag="VLE22_18930"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative N-acetylmannosamine-6-phosphate
                     2-epimerase"
                     /protein_id="HSB16534.1"
                     /translation="MKPFSNVLLALHQRLIVSCQAPDGDPFHDPESMARFARTAVLGG
                     AAGIRANGAEDVRAIRAAVSVPILGIDKRLIEDGRILITPTFEGAHALAEAGADIIAL
                     DCTARGRHYGAIERLQRIRAELRVPVMADCATLEEAKTAAAAGAEIVASTMRGYTDDT
                     ADVTCFEPFYVAEWVRELDTPLIVEGHVSTPDEAAAAFAGGAFSLVIGTAITRPEAIT
                     RRIVQAIECQVRSGKHRFFLGIDLGATNTKYGVVARDGRLVARASVPTPSDGGREVLL
                     AHLKRVAGMCRELAAQAGVEPSALGVATAGWVDPGTGAVAYATDNLPGWTGAPIAAEL
                     GPVAGLPVGVENDANALAVAEKYFGAARGADDFVCVTLGTGVGGGCYVAGRLNRGPHF
                     LANGIGHITIEYGGLPCTCGRRGCLEVYTNAAALLRYARGGFASAEVVIREAGRGDGP
                     ARGAIRTLAGYLAAGLTGIIHLLDPSLLVIGGGLAENNPMLFDDLKAAFAREVTAWDH
                     RRIEVCPSPLGYYGGVLGAAAAAIEKLA"
     gene            complement(40782..41801)
                     /locus_tag="VLE22_18935"
     CDS             complement(40782..41801)
                     /locus_tag="VLE22_18935"
                     /inference="COORDINATES: protein motif:HMM:NF018123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HSB16535.1"
                     /translation="MRSSVALGVFLALASGACNGLFTTPMKLIPRWKWENIWLVFILI
                     ACVAMPAAMVASGVDAVLVLTASPPNAIACAAGFGLAWGFGAIFFGLSVHHLGVSLAN
                     SLVIGLSSALGSAVPLLLSGNLRFDLSQIVLFIGVATFLAGVWFCGQAGRLRESSASV
                     SSRSGGSLFSAYALCIGAGILSAVFNIGYSLALPIAAAGERLGYSSFDSTNLIWLLML
                     GGGAIPNIAYCSMLLRRNGSAALFSGPQPGKAWGLSLLMGLLWGASIFLYGAATPMLG
                     DIGPSIGWPLSLAVGLVVANVMGLWLGEWRGADPRALRLMRSGILTLLAAIVLCAVSS
                     SFGKA"
     gene            complement(41866..42621)
                     /locus_tag="VLE22_18940"
     CDS             complement(41866..42621)
                     /locus_tag="VLE22_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16536.1"
                     /translation="MHWAGKSVVVSTLLAGLSTLPAAAQTVISARSGLVHYVEGAVFL
                     GDQAVIGQPGDYPLIPEGQLFRTGAGRAEVLLQPGVFLRLAEESSVRLLSASITRAEV
                     EFLAGSIIVDAGQLRGSSVSVVANGTPAVLRKSGLYRFDAAAPPCIRVYDGKAQVGLG
                     SQSVEVEKGKLLSLDGSGAPQKFDRKDTDEFARWSDSRKEYLTMAYLEASQDSSDPEL
                     RRSISDASVTGPPTTSGRSRRTTRSGGARRRSR"
     gene            42923..45226
                     /locus_tag="VLE22_18945"
     CDS             42923..45226
                     /locus_tag="VLE22_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16537.1"
                     /translation="MFQGVWKAALAAVLLLSACGCQYVGRTSRATRESMGYFRNDAWT
                     IIGPGGGGAQFYPAVSPHDANRVVVGCDMTGSYLSTDGGRGWRMFSLREPARFFVWDP
                     ASPNILYAQTSALWRSADTGETWELVYPDPSMVTGVAMPGDHGDGWIMTSAGSFDTVA
                     GLAIDPADSNILYAVVTSSRGSYLRVSRDWGRSWTWAADLPQGSKAIYIDPDSPRADR
                     TIYVVGDTYVLSRVAGTWRQGAPTPGYAVMTKVAAGFADGALVIYAISARGLLISRDG
                     GQNWVDSYVSMQLVAVATPLWSGRTAYVSYGELPEGGRSYFGVAKTENAGISWQYPWK
                     EAGMKSPDVDDGWMSARFGPDWPGPPVSLGTAPDNPQICYGSDYGRTLRTTDGGSTWR
                     SAYSYQLADGSFSTRGLDVTTCYGVHFDPFDAQRMFISYTDIGLFVSDNGGKGWRSST
                     GVGVPWEWVNTTYWVEFDPEVPGRMWAVMSGTHDLPRPKMWRTKSPSSYRGGVAKSED
                     GGRSWRTLMNGMPQTAATHILLDHRSPAGARVLYVAGFGKGVFKSTDGGESWQIKNNG
                     LPAFEPFAWRLAMDRDGILYLVVARRSENGSIGNDQDGALYRSRDGAETWEKMRLPDG
                     TNGPNGLAIDPDDPRRLYLAAWGRRAGSQAAGGGIFLSTDAGETWRAVLDKDQHIYDV
                     TIDPRAPALLYASGFESSAWRSTDRGETWRRIRGYNFKWGHRVIPDSADIGKIYITTF
                     GGSVWHGPAQGDDSAVEDIVSPPALRY"
     gene            45369..46067
                     /gene="aqpZ"
                     /locus_tag="VLE22_18950"
     CDS             45369..46067
                     /gene="aqpZ"
                     /locus_tag="VLE22_18950"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HSB16538.1"
                     /translation="MSRRAAAELIGTFWLVFGGCGSAVLAAAFPGLGIGFLGVALAFG
                     LTVLTMAYAIGHISGCHLNPAVSVGLAVGRRFPASELLPYITAQVAGGILGAGVLYTI
                     ARGKIGFDLAAGFAANGYDAHSPGGYTAGACLVAEVVLTFMFLMIILGATDRRAPQGF
                     APVAIGLGLTLIHLIGIPVTNLSVNPARSTGPAVFVGGWALEQLWLFWVAPLVGAALA
                     GIVYPAVAGDRESR"
     gene            46149..46403
                     /locus_tag="VLE22_18955"
     CDS             46149..46403
                     /locus_tag="VLE22_18955"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HSB16539.1"
                     /translation="MMHFLWMCLIGLVAGALAKLIMPGKDPGGIIITMLLGIAGSLVA
                     GFLGRAVGWYEAGQGAGLIMSVIGAILLLAVYRLMKRGSA"
     gene            46462..46977
                     /locus_tag="VLE22_18960"
     CDS             46462..46977
                     /locus_tag="VLE22_18960"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HSB16540.1"
                     /translation="MPIKKKICLLGAEAVGKTSLVRRYVLGIFSDRYLTTMGVKVDKK
                     ILHVAEEEVHLIVWDLAGEDELTQLRTDYLRGASGYLLVVDGTRIATLHKALELQRKA
                     ADAVGSVPFVLVINKSDLADEWFSLQDELEESGCRFWPLCTTSARLGTGVEEAFLMLT
                     RLMRAHQAEPG"
     gene            47001..48728
                     /locus_tag="VLE22_18965"
     CDS             47001..48728
                     /locus_tag="VLE22_18965"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSB16541.1"
                     /translation="MSNIEILGLLSALDNAVLRKDEKGRWRLVETPPEWFIKLYPGAE
                     SPSGLHAEEWSPFLETFLENAGDFLCESPGGRLKSGPWTESTPDGEEIDMEATILRHK
                     GKTLLVLTHRGEEYGERRQILQKAREKSLKYQKLERVHDELALVLNQLEVGALTTDAA
                     GRCVFLSEPARRLFDMDEKEVLGRRWLDLKILDSAGRRLLELMARLLPAERTRVPLHW
                     ERDDGRAFWMEVDLRDHPKDARKKIVFLYDVSELHSLRRLLGDQGQFENLVGKNDAMR
                     RLYQSIENLTGVDTTVLIEGETGTGKELVARAIHNRSERRARPFVAINCAGLTESLVS
                     SQLFGHRRGAFTGALQDSPGLFEAAEGGVLFLDEIGDLPLAVQTSLLRVLQEREITRL
                     GETRTRKVNVRVLAATHRNLTEETERGNFRADLLYRIRVARIQIPALRDHLEDIPLLV
                     SRFLADFRASTGKMVLDVSAEAMRALMRFPWPGNVRELRSAIEFAVINCRRPVLALDD
                     LPPEISARPTGTMRAPTPEAPPADEKERILSALRKAGGKRSAAAKLLGISRATLYRRM
                     ADLKIPAGV"
     gene            49142..50194
                     /locus_tag="VLE22_18970"
     CDS             49142..50194
                     /locus_tag="VLE22_18970"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HSB16542.1"
                     /translation="MRSAVLFVLLLCAAHAGRADQITLKNGDRVTGAIIKKDGKTLTL
                     KTDAFGVITAPWDQVAAIQSDAPLNVVLPDGKTVQATVQTSAGRVELKETRQTLATEE
                     VVAIRNADEQKAYERLLNPGWADLWAGTATIGFAGTQGNAQTRTFTTAVNAARVTNTD
                     KTSLYFNAVKASALLEGVSAGTAQAVRGGWAHNHTISSRFFVNTFNDYEYDRFQDLDL
                     RFVLGGGLGYNAWKGERGRFDLVGGLAYNREKFSPPAPASEFTRNSAEAYWGEDYNYR
                     LTEATSLVQSFRMFNNLSDTGTYRMNFDLGANTRLFKWLTWNLGFSNRYLSDPVPGRK
                     TNDILYTTGIGVTFAR"
     gene            50246..50797
                     /locus_tag="VLE22_18975"
     CDS             50246..50797
                     /locus_tag="VLE22_18975"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HSB16543.1"
                     /translation="MNHQRLMHLFCGLAVTILLATFSPMWAQQAGTGFLKVKANTGRA
                     GVFVDGKYLGPAANFAMARKYVVAAGEHDLVLREPRYQECKTTVKIDAGKTTTVAQSL
                     QPRALATPPFGRLRLTGFEKYAAVYVNEGYIGHADEFSAPGQGLLLNPGDYNLKVTSQ
                     AGSTLLEQKITIRQNEVARVQAK"
     gene            50915..52564
                     /locus_tag="VLE22_18980"
     CDS             50915..52564
                     /locus_tag="VLE22_18980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB16544.1"
                     /translation="MKAGRQKDSLPNDGTGELRPDRPANDPASDDLLPDLDVSGNEIL
                     EETDIAPSPSAEDVEELGEFELDAESWASTDDPIRTYLHEMGSFSLLTREGEIDLAKR
                     IERGRARVRKALSRVPLVIDEMLKLVEAVQQGRVTVRDVLVTPETADIDDSGAKQREE
                     LLQWLSEIAKYQERAERVRQKLQDGWRGMRPKQLRRLRYNTARSLVRLSRIYRQVPLR
                     PEFERKLIELIRGAVAQYRPVEREIAKIQRRLEQSVLAGSAGQKEELRTSLQQLIQRL
                     RKLEKDWGWGALDLRRTLRIIERGEQEAETAKKQLVEANLRLVVSIAKRHTDRGLQFL
                     DLIQEGNIGLMRAADGFDYRLGYRFSTYATWWIRQGITRALADQARTIRLPVHMIEAI
                     RRLAGAQHELRQELRRDPTPGELARKLEISVSKVRRILRAAQEPISLETPIGEEMDSS
                     LGDLLVDHEAVSPSQSIISLNLREQTAELLRTLKPREAEILRMRFGLLDDRVYTLEEI
                     GLRFAVTRERIRQIEMKALRKLRGHGRSHQLRTFLGGTSRG"
     gene            52688..53038
                     /locus_tag="VLE22_18985"
     CDS             52688..53038
                     /locus_tag="VLE22_18985"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HSB16545.1"
                     /translation="MAAGVKTDADMLDKAGSAKGRAELSEKTGITGTLILKWANDVDL
                     MRISGVGPQYAELLEAAGVDSVKELAQRNAANLATKLAGVNALKHLAGTSPAESQVAD
                     WISQAKKLAPKISY"
     gene            complement(54342..55787)
                     /locus_tag="VLE22_18990"
     CDS             complement(54342..55787)
                     /locus_tag="VLE22_18990"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB16546.1"
                     /translation="MNRRDFLKSTLAAAVRPGKRQPNILVIMTDEHNPRVAGFHGNSL
                     ARTPHLDRLAERGVTFEHAYCNSPLCVPSRLSFTAGKYIHRISAWNNSCRLPSDDHPS
                     LPHLLNGVGYESFLCGKQHYDAAHRYGFVEIGGNMNSSNMTGRGGRRAADDESVNVKS
                     CRDRTKDFHPGDDSSILSHDRRVTAGALDLISRRKRAKKPFFLFLGYLAPHFPLIVPE
                     RYWRRFEKKVPMPEIPDGFLDGMPLNYKHLRRGFGLPNMDAAATRRGRELYHGLTEWV
                     DDEIGKVLGALDRSRLARDTVVVYTADHGENMGEHGLWWKNCVYEQAARVPLIVSWPE
                     RWKGGQRRAGACSTVDLVRTLAEIGGTRTPDDWDGTSMTRWLDDTKSPWKDRAVSQYY
                     GHNIASGFAMLRTGDWKYVYHSAPDASHPAERELYNLKDDPGEFRNLAKESAHGSRIQ
                     TMHAALVKELGEHPDESEQRCRAETARGYDG"
     gene            complement(55805..56986)
                     /locus_tag="VLE22_18995"
     CDS             complement(55805..56986)
                     /locus_tag="VLE22_18995"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB16547.1"
                     /translation="MKIPTLSRRHFLSASAAAPATGALQAAGMKASELPDLAIKEVRV
                     YHTDHGLLAGVVTASGIEGNYTLGTRYWHPDWNNEGWLGYAKGLLLGKSALDRPALTS
                     QWVPAKRRRGQSSYASAIDNCLWDVLGKAVGLPVYQILGAYRDKVLAYASSQHLRTVD
                     EFVESALKCKAEGFRAYKIHPPEVPPHGGSNYRLDMEVSKAVRKAVGEDFLLLMDPVG
                     VYNRDEAMRVGRLLDELGFVAYEDPIPTTDVEGYVQLCQALDVPIHAGEFIFSIYDYS
                     EYVRRGAVDVLRFIVDNVGGITGGMKISAMAECFGMECAPHNWGDTLDHAVHFHCELA
                     MPNNVFFEMTVPQGVYDRPWMKDRIRVAKDGYVYAPTKPGLGYEIDRNVLDNLTKRLE
                     R"
     gene            complement(57005..58867)
                     /locus_tag="VLE22_19000"
     CDS             complement(57005..58867)
                     /locus_tag="VLE22_19000"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB16548.1"
                     /translation="MTRREFVCLLASPASAQLTRSAEVVPGAVNGLIFTRGGRRLAVY
                     GDPRPSPASAEQVLLTHSRRDVVWAARELQRKGATVIAPATEAELIAHPGKFWQSFEK
                     ARYHDYAMLNSRVLTEPLPVARAVRGGERIEWQGLVIDVLDTPGYSPGAVSYLVTVDD
                     RRIACTGDLIYGDGQLFDLWSLQDAVPEAKLRGYHGYAARAGELIRSLRAVAGQKPDV
                     LVPARGPVIERPTEAIDNLITRLKTPLREHFTTDALRWYFKDDNLRLRAAKLLEGEVP
                     EWMPMAETRALPGWAVTISNSRLLLSESGAAFLVDCGSDDIVAKVAELRDSGRFRTLA
                     GVFITHYHDDHTDRAQVCAERFGCPVYFTSELRDILENPSAYRAPCLTTFPIRSGKAL
                     AHGHRWRWHEFELSVSFFPGQTLWHDSLLAKKDGGEAILFVGDSFTPSGIDDYCLWNR
                     DFLSPGEGFWNCMEQVRRLPRDTWLVNQHVEPAFRFTEEQLNYMQRSLQRRAAAMQPL
                     FPWPGIDFGLDEQWCRLAPYEVKAAPGRPFQLQAFVWNHAPTVQEFTVRLNLPAGWKA
                     SEPAYRIRTQAGGSASAAMTITPTPEANGLAVITADVEFSSYRLREWTEAMVRL"
     gene            complement(58885..60318)
                     /locus_tag="VLE22_19005"
     CDS             complement(58885..60318)
                     /locus_tag="VLE22_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16549.1"
                     /translation="MRALLTLALTASAFAQIDIGSRREIFVDRHLIDRLDGASLRLQT
                     PVEKGTVLALDRPWEGAFCAYFTVLKDRNRYRLYYRCVPNSGQDGRIEEATCYAESPD
                     GIRWSRPDLGLYEVQNTRQNNVILAQKAPFSHNFTPLLDTRPGVPPSEQYKALAGTSE
                     SGLAAFVSADGIRWRKLREEPVLTPARETRYDSQNLAFWSESEGSYVCYFRTFKVLPD
                     KRHVRWVTRTTSTDFVHWDTPVEMSFGDAPPEHLYTNQTSPYFRAPHIYVAIAARFFP
                     GRQVLTEDEARAIHVDPKYFQDCSDAVLFSTRGGQRYDRTFLEAFLRPGLGAENWVSR
                     TNYPALNVVQTGPAEMSFYVNRNYGQPTAHVGRYALRLDGFASVNAGYAGGEMLTKLL
                     RFRGRTLEINYATSAAGSVRIEIQDGEGKPVPGFSLADCREIIGDQVVRNVSWAGGGD
                     VSNLAGKPVRLRFVLKDADLFSLRFRD"
     gene            60389..61255
                     /locus_tag="VLE22_19010"
     CDS             60389..61255
                     /locus_tag="VLE22_19010"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB16550.1"
                     /translation="MNRRMFLAMAALPAAVRAEDWPAKSGVFRQDNLVAWCIVPFDAK
                     QRGPEERAAMLERLGIKRFAYDWRDKDIPTFDQEIDALNKHGIRLQAFWFPCGLEPAK
                     DKNAGIILDLLERRKLKTELWFSLSGETKELDAMPLEKRVAEVAESVQWVAGRAGKIG
                     CSVALYNHGGWFGEPENQVAILKRVRMKNVGIVYNFHHGHDHIERFPELFALMEPHLM
                     AVNLNGMRKGGPMILPIGDGDRELEMIKVIRRSRYRGPIGILGHREELDAEEALRLNL
                     EGLKKLIPITGS"
     gene            complement(61242..61676)
                     /locus_tag="VLE22_19015"
     CDS             complement(61242..61676)
                     /locus_tag="VLE22_19015"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSB16551.1"
                     /translation="MRKTIVAAVLMLSVAVTLAAAGPEDEVRAAEKSWTTAVAALDYA
                     ALDKLLHDQLIYAHSTGVVETKAEYLAKLKSGAQKYDSIEHLSLTVKPHGNAAVAHAV
                     VRMQGKSKGEPFDNRLMMLHLWVKSGGKWQLAAHQTTRLTNP"
     gene            61753..62652
                     /locus_tag="VLE22_19020"
     CDS             61753..62652
                     /locus_tag="VLE22_19020"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSB16552.1"
                     /translation="MPELPDVVVYIEAMDERVKGRRLDRVRIAGPFLLRTAEPPVQEA
                     EGKTVLEVRRLGKRICIGLEDDLWLVLHLMIAARLHWRKPGAKVDGRRTPAAFDFDGG
                     SLVLTEAGTQRRASLHVVSGKDGLRALDPGGLEVLDAGFEDFAAALTAHNHTLKRALT
                     DPRIFSGIGNAYSDEILHRARLSPVALTQNLTADDTRRLFAAVRETLLEWTDRLREGR
                     RGGFPENVTAFREEMAVHGKYGKPCPRCGAKVQRIRYASNETNYCAVCQTGGRLLADR
                     SLSRLLKQDWPRTLEELEGLKGR"
     gene            complement(62687..63901)
                     /locus_tag="VLE22_19025"
     CDS             complement(62687..63901)
                     /locus_tag="VLE22_19025"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB16553.1"
                     /translation="MENHSSTDTERRGFLKAAAGSLLILKPETAFGSQANSAVEVGLI
                     GCGGRGNWIGEFFPEFAGARVVALADVIRDHLDKTRDKFKVDASRAYYGPDAYKELAN
                     SKLDGVVIETPTYYHPEQAAASVDAGKHVFCAKPIAVDVPGCRTILDSGKKAAKAKLS
                     FLVDFQTRARAVYQAVAARVHRGDIGTPAMAQVFYYAGRVIQDRSKPGMDPGLARIMN
                     FYMDRVLGGDIIVEQNIHVIDVANWLLQGHPLKASGTGGRTDWSGTPYDAGDAWDHFV
                     VTYWYPNNVHVNFSSHQLTRSFADLCVRVFGIKGAADTHYNGLIRVTGDNEWHGTDKD
                     DTFRGGAIENVKTFIESIRTATPVNNVADSVDSNLTAILGRTAAYQQREITWDEMMRS
                     NEKWEANLKLRW"
     gene            complement(63928..64746)
                     /locus_tag="VLE22_19030"
     CDS             complement(63928..64746)
                     /locus_tag="VLE22_19030"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB16554.1"
                     /translation="MIHRHTRPYAFLLATLAAVATIAVAQRTSPVGYDDTPVLPGQKW
                     KVHDIARPRPHVVTPGRTCGAPSSDAIVLFDGKDLSKWIGRRKGKDVAPGWKVRDGYM
                     EIASGTGDLVTKERFGNCQLHIEWTTPANSRDSSQWKGNSGVLLMDRYEIQVLDSYEN
                     PTYADGGAGSIYGQWPPLVNPARKSGEWQSYDIVFEAPKFENKKLVKPGYVTVFFNGV
                     LVHHRQEIIGRMAHRVVGTYAPHEPEEPLGLQDHDVPVRYRNIWIRRLTGYDQP"
     gene            64907..65305
                     /locus_tag="VLE22_19035"
     CDS             64907..65305
                     /locus_tag="VLE22_19035"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HSB16555.1"
                     /translation="MAETPHSELSRRERQIMDVLYKLKRATVGDVLSQLPGKPNYSTV
                     RAQLRVLEEKGQVRHEEEGLRYVYAPTAARDAVRKSALKHLVETFFDGSPEKVVATLL
                     GGESSKLSREDLDRLSGLIEKAKNKKGSTR"
     gene            65302..>65767
                     /locus_tag="VLE22_19040"
     CDS             65302..>65767
                     /locus_tag="VLE22_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16556.1"
                     /translation="MNAHFLTPDFWLSVLADGALKGSVILAAAAVTARLMWRQAAALR
                     HLVWAAAVVSLLTLPAANRFMPAWYNGAYEKVALLAGGIDAGAVLEPPAEDPMLVAAG
                     GVQKKSNNRTWILIAIWTVGFGTAVLTLGAGTLRLWRLVRGATPLFVRRWVLL"
BASE COUNT        12462 a        19685 c        21367 g        12253 t
ORIGIN      
        1 tggcggggag caaaaactcc ccgaaattct ggccgcggcc cgcaccgggc cagaacatcg
       61 caatcgccga gcggaacgga tactgcgctc gaatctcaac ccacgcgaac tgccttccgt
      121 cagggaaatc ttcgggcgca ctccccaccg ggccgcccgg ccaggacagg tcacctggat
      181 ttccggtatc gagacaggcg gcgagcgttg cgcaggtgaa cgtaccacgg gtagcggaaa
      241 tgaactccag gttctgcagg tcgtccggca cgaagcctac ggcgttccgg cgaatccgct
      301 cagagatatc gcggatcgtg acagtgcaat tgcttgggta gatgctacag ttgttgccgt
      361 gcacaatggc gaagcgcgtg ccatccttga tggcgctggc aagcgtgtga tagagccaca
      421 tgccacggga gatctcgaag atcgagatca gcatgaagat caccggaatc ccgaccagcg
      481 tgaactccag cagggtgctt cctcgttttt tcttgctcgg ctccatagct acggcctcgt
      541 tcttccgtac atccgcgact cggcggtccg actgattgtg gtcttaccgg tgagcagccc
      601 ggggatcggt atcagttgga cggtctcata tctcacctgc acgcgcgaga ataacttccc
      661 gtcggcatcg agcccgctgg aggcgtccac ctggacgctg gccggcaggt cactgcacga
      721 gaggtccgta taggcgctcg ctgggtccgg gacattgggc aaccgctcca tttccctcaa
      781 caccagctcg cacgcatatt cccggttccc tgccgttccc tcgcttcgcg ccgtcgtgat
      841 ggcggccact ctcgcggcgt tctgcaccgt gatgagagca taagcataga agcccatgtc
      901 cagcgcaccg acgaagagaa agaagatcca gggcgccatg agcgatagtt cgacgattgc
      961 gtgacctctc cgagcttcct tacagggttt tttcatgctc accgtcccct ctctctcgac
     1021 acctgcttcg ccttcaaccg taagtcttac gtcgattgtg tggcaggtac tgcgaacgtg
     1081 tcaatgtatc ttagggatcg atcgaaacta ggagtactgt accccagcag gacgccaata
     1141 cccctgttaa cccgtctcgg gcgtagactc agatgagcga aagcgcttga agccatgatg
     1201 atgatttggt ctctcgctct gctggttcaa acggcaacgg cgccgaacgc gctgccgggc
     1261 acttctcctc tcctggagga ggccgactac gctgtgcgga tggtcgaggg cattcaccgc
     1321 ttccttgatc gcgaaaccgc ggcggctgcc gctgctcgcg aatctcgatg gaagcgtgct
     1381 ttccggacgc ggaacgccta cgaggcatcg gtcgcgccga accgcgaacg atttcgaaga
     1441 atcatagggt tagctgacgc cagggtgcca tatgacgcgc cgaggctgga tgtcgtcttc
     1501 ggtggcgccg tgatagtcgc gaaaggttct ggctatacgg cgcgggcggt acgctggccg
     1561 gtgctgccgg gcgttgacgg cgaaggattg ctgctggaac cggaccgcga acccgtggct
     1621 cgcgttgtcg cgctgcccga tgccgactgg acgccggagg cgatcgcggg aatgtcgcca
     1681 gggcttccgc cggaagccca gtacgcccgg cgtttggccg aacaaggcgt tcttgtactg
     1741 gtaccggtct taatcgatcg gaaggacaac ttctcgggga acgccgcgct cgggtgtctc
     1801 accaaccagc cgcaccgcga attcatctac cggatggcgt acgaagtcgg acggcacatc
     1861 atcgggtacg aggtccagaa ggtccttgcc ggagtggatt ggttctcgcg gagcgagaag
     1921 aaaacgccga tcggagtctt tggatatggc gagggcggtc tgattgcttt ctacagcgcg
     1981 gcggcggaca cacggattga tgcggccggt gtctccggat acttcgggcc gcgagaaggg
     2041 ctggccaacg aaccgattta ccgcaacgtc tggggtctgc tacgcgagtt tggcgatgcg
     2101 gagatcgcag gattgatcgc accgcgtgcg ctggtagtgg aaactgctcc gggacccgtg
     2161 atcaacgggc ctcgtctgga gacgaaggag cgtaaaggcg ccgcgcctgg tgcgctcgtt
     2221 ggaccggatc cgcgtcaggc gggcaaggaa atccaccgcg tccttcgctg gcacatgcaa
     2281 ttacccggta ctcctcgccc actcttttcg ttcagtctcg gcggtacaag ccggatcagc
     2341 gagcatggga cgcttaatgc gtttcttgaa gcgcttggcg cgaagtacga tctaaatctg
     2401 cacggcgctc cgccagggga tcttcggacc ggcttcgttc ccgaagcgcg gcagcggcgg
     2461 cagttcaatc agcttgtcga ttttacgcag gcggtggtgc ggggtgccga agcggcgcgt
     2521 cggaaattct gggcgaaggc cgacgcatcg tcggtggaag cgtggcagcg cagcaccgag
     2581 tggtaccgcc gctacctgtg ggaggaagtg atcggccgga tgccggaccc ctcggagccg
     2641 cttgaggcgc gcactcgggg catctacgaa cggccgaatt ggacgggata cgaagtcgta
     2701 ctgccggtat ggccggacgt gatcgcgtac ggtgtgctgc tcgtgccaaa ggagatgaag
     2761 ccgggcgaac ggaggcccgt ggtcgtctgt cagcacgggt tggagggcag accgcaggat
     2821 cttgtcgaac cgaagacccc cgcggcggag aatgcgtacc ggcgcttcgc cgcgcggctc
     2881 gcggatcgcg gcttcatcgt atacgccccg cagaatccgt acatcttcga gacacagttc
     2941 cggcagatcc agaggaaggc gaacccattg aagctgagcc tgttctcgtt catcatcggc
     3001 cagcaacaga gggcgctctc gtggctgtcc tcactgccgt tcgtggatcc gcaacgcatc
     3061 ggattctacg ggctcagtta cgggggcaag acggcgatgc gagtgccgcc gctgctcgaa
     3121 cggtacgcgc tctcgatctg ctcaggcgac ttcaacgaat ggatctggaa gaacacgagc
     3181 gtggatcagc cgttcagcta catgtttacg caggagtacg agatgcccga gttcgacctc
     3241 ggcaacacat tcaactacgc cgaactcgcc tcgctgatgg cgccacggcc cttcatggtc
     3301 gagcgcgggc acagggacgg cgtcgggatc gacgaatggg tcgcgtacga gtatgcgaag
     3361 gtgcgccggt tctacacctt tttgaaaatt ccggaccgaa cggagattga atatttcgac
     3421 ggcccgcaca cgatccacgg tgtgggaacc ttccggttcc tggagaaaca tttgcgctgg
     3481 cctgagtgac ggctcagtgc tcgccggctt cgctggccag acgttcgtag acagcgcgcg
     3541 cttcgccgaa ctggccgagc acgtgggtct tttcttgctc cgaacgccac cagagccaat
     3601 cagcggtcat gcggctgagc ttgtcgatcc attggacgaa gtactcggcg gactcccggc
     3661 tccggatctt ctggccgccg acgtacacgc gcaccgcgtt ggtcgctgcc tggaggaagg
     3721 tcgcgtcgaa gtacggatgt gcgggaccct cggcatagag cgacaaccag ccgctgccag
     3781 tcagcttcag ttgggttttg agcgacgcgc ttttcttgtc cgcggccaac gggaccacca
     3841 tcagcacttt gccgttgtga tggatttcca ccttcgagag aggagcgatg cagacggccg
     3901 aggcctcgat ggaaatggtt ccgccttcgg ctggaagtct caccgtttcc cccggaatct
     3961 tcccgtccac gcggaattcg agcagcgggc cggtcgagaa gaatgtgcgg cctccgcgga
     4021 ttgcgtcgag ccaggcctcg gcagaaacgt ctgacccgag ccacgcgtac gcgcgggcgc
     4081 tgccgatgag tttgctgcga tgcaggtcgg tgatggagtc ttcgcccccg accggggcga
     4141 ccggcaggtc gttgttgaga acgtggtgcc agacgatcaa gccgccgcgg ccggcgtccg
     4201 accattcgag cgcatccacg gtgccgagcg cggcgtcgac cgggaacgcc ttcgcattac
     4261 ctagcccagc cggcagcgga tcggcatcgc cgtacggatg tacgtaggtg gtgacggcgc
     4321 cttgcgcacg ggccttgcgg aagatgtcgg tgttggtggg atagaggctc tcgatggcag
     4381 tgccctcgta gccggtggtg aagggcgaca tgagatggtc gcgcagaccg aacagggaag
     4441 tgtggccgtg gaacggcggc cggtattcct ggccgaagat cagcttgctg ccggatcggc
     4501 cacgggacgc cggatgttct ccaccgccgg gagcgaagta ctggtggtcg agcacgcgat
     4561 tgtctttgtt ggcggcctgg gcgttgagga agtcctggtc ctcggcatcc gacatcagca
     4621 gcagattctc cggcgtgttg cggaggttgc cgccgtaatt catgtgaacg tgcatcgaac
     4681 cgttgtgcca gccttttgcg gagaagtcgg cgaggcggcg cagagtgagc gttaattccg
     4741 cgtcgcctcc ggcaggcact tccgtcttcg ccaaggccgg aatgaattca aaacctttga
     4801 gcgcctcgac ggtcactgtc ccggccggta actcgaccgt gaacgcgccg gcggtgtgaa
     4861 acaggtggcg gccggacgcg ccgatgcggg agtagacatc cgtgggcgtg tagaactttc
     4921 cgtcggcggc attcagatgg acgcgcgccg gtgtgggctt atccgtgcct gcgtcgatca
     4981 ctcgtacgcg cagcgtgccc atggggcgct tccatttgcg cgaggtaatg cggataaccc
     5041 tgtttgcacc tccgtacgtt tcgagcagcg cgagctgcgg tagcccaccg tcgttcgaga
     5101 tgtaggcaat ccactccccg tcgggagacc atcgtgggtg aaagcggtcg aactcaccga
     5161 aggtgagctc gtacggcgca ccgccttcga tcggaatcac gtagaggttg tcgaactggt
     5221 cggcggcgcc cccggtggag gagtagagga ttcgcttccc gtcgatcgac acgtgcggcc
     5281 gggtgcgata cagagtctgt tcggcgagga ttcgctttgc cttgaggatg ccatccggtt
     5341 ccgcgggcat gcgccacagg tcaccggctc cgagggggac accgcgattc gaaacgaaca
     5401 cgatctcctt gccgtccgga gcccaggcgg gttgagtatg catgtcccac ggaccaaagt
     5461 agaggcggtc gcgagggtat tggttatcgc gagtgaccgc aatctcctca ccggcccaat
     5521 ctccgtcgcg aatcggacgc acgaaaatgt tgaagtgacc atttggcttg gaggagacgt
     5581 aggcgagccg cattccatcg ggcgagaaga tggggtcgag gtagacctga gagtcactgg
     5641 tcagcgcccg ggtccgtcca ctcttcagat cgagaatttc cagttggatc ttcgccccgt
     5701 cgtcatcggc ggtgtaaacg atccagcggc catcgggcga ccaggcggga gacgagtggt
     5761 acgtggttcc gtaggtcagt tcctccgctc cgcccgtgcg cggatcgacc ttccagatgg
     5821 acccctgcat cgcgacggcg atccatttcc cgtccggcga ccagcacggc gcccagggcg
     5881 tcgagcccgg cgccggcggg atgtagtagt tgtgcatgta gttgccggct tgccggacgg
     5941 cagggtacgg gtttggcctc gcccaaacgg agagcgcggc cgcggcgaga agcggcagtg
     6001 cgtttcggat cacatgcgga agaatactag ccgccgcggt actcaagcaa gggggcggcg
     6061 cccgcacggg cctgcacgtt gcgcttccaa tcttcaagcg ccggtccagc gatcggctgt
     6121 ccacgtttgc gcattgcatc catttcggaa gcggtcagcg gctggaactc tcgcgcaacc
     6181 cggacgtcgt cctcgatctg gccgatggtc gtgcagccga tggcggtgca atggacaggg
     6241 aggctcagca cgtagcgcag gcactcccgg acgctgaact cggaaagcag cttggcgttg
     6301 ccaaagttct tcatgccctg aatgccgagg ccgcgctcca ccgcaagggg caagacgagt
     6361 tgctcgaagc tgaggtaaga cggatcggcg gcgtggagcg gcatgaggat ggtgtcgtat
     6421 ttgtcatagc gcttcagcat ctccaggtgg acgtgagggt cgtggtggcc ggtgaagccg
     6481 atgaagcgac acttgccggc tttcttcgcc gcttcaaagg cctcgatggc gccgccgggt
     6541 gcgaagatcc ggtccacgtc ctccatttcg ctcacggagt ggatctgcca gaggtccagg
     6601 tagtcggtct tcatcgcctt tagcgacaga acgaggtcgg cttcggctcc ctcgcgcgtg
     6661 cgctgaacgc tcttggtcgt gatgaaaacg ttctttcgga actcgggcag gacggctccg
     6721 tagacttcct ccgaccgtcc cttgccgtag gcgcgtgcgt tgtcgaagta gttgacgccg
     6781 aggtcatagg cgcgacgcac caccgccttg gcctcatcga gcggagccag gtccaaacgg
     6841 cctccagcct gaccgatgac ggtcagcttc tcgccggtct ttccgaagac acgcttcgga
     6901 atgtccccgg ccttcagctt ctgagaccgg gcagccgggg ccaccagacc agcgaaagct
     6961 ccaccaacga atacgcgacg tttcatggtt gctcctccga ggcttacctg catgataatg
     7021 cagttcgtct cgcccggtgc ggtgcgtacc tcacggcagc agccggtaga agcggaacag
     7081 gaagttatcg atgggaagaa tctccacatc gcggaagcct gcttccatgg cgtagcgccg
     7141 cagcgtagac ggccgcatgg ccgcaccggt cgcggcggaa ggttggtcgg ccatccccga
     7201 gggcaggcag tgcagaacgc tgaacccgta catcaggcgc tcgacgacgg acgctccggg
     7261 ttggaactcc tcgccaaccc gctcgtccac taccagcacc gtgccgtcgt cttttgccag
     7321 gcggcgcatg gcgcgcagcg cgccaaccgg gttcgacatg tcatggatac attcgaacgc
     7381 ggtgaccagg tcgtactgtc ccgcgagccc ggggtcgccg gcatcccgga cgaagaaccg
     7441 cacgcgctca ccaatagcgt actgtcccgc attcgcccgg gcgagttcga tcgacggccc
     7501 gtcgagatca aagccgtcca ctcggacgtt cggatagtgc agcgcaatgc cgatgcttga
     7561 ccaacccgcg ccgcagccga catccgccac gcggggcgga ctgacgccca acatgcgcga
     7621 gtggacatcc ggaatcgcag gcagccactc cttgcccagt tgatacagga acgcggcgcg
     7681 gttgattcgc gcctgtcctt cacgtacgtc agcgccgtag gctgcgaacg ggaccccgcc
     7741 gccggtgcga aacgcggtca gcagcgcgtc aatcgggcgg actgctccgg cgacgatttg
     7801 cgcgaggggc gcgaggtagt tcaggctatc gcgttgggca aggacctctt catggccgga
     7861 cggaaggcag aaacgccgcg cgccattccc gccggtggtc tctttcacat ccaggatgcc
     7921 cgccatggcc tgctgttcga gccactctcg cgcgtagcgc tcgtgcgtgc cggtgcgtgc
     7981 ggcgagctcc gccgaggtca gcggcccgtc agcggaaagc acttcataga accccagccg
     8041 gtcgccgaga tggattgtga agatatcgaa ggctccaagc gccgattgga agagccgttc
     8101 gatgaatgca tctcgtcgcg ccgccgtctg ttcggtttcg aattgtgtcg ctgttgccat
     8161 ggttgcctcc cacgtgctcg gttcttttcc gtaaactggt cttaaccggg aggggttcgg
     8221 aaccgagcga cttgcccctc atctgggaat gcgaaaaaac ggagcgggac ccgacacgca
     8281 cgggcgattt ttttcgatga gcgaggtctc cgaaggcaca gggccggccg acgtgacgca
     8341 gcttctgggg gagttgcgct cgggttcgcg ctcggccgcg gaacgtcttt tcccccttgt
     8401 ttatgaggaa cttaggcggc tcgccagggg acaactccgg cgggagcatg gtgaccacac
     8461 actccagcca acggcgctcg ttcacgaggt atacctgaag cttgcgggtt cagcgcccga
     8521 gcggtggcgg gaccgagcgc acttcctcgg gatcgcggcg cgggcgatgc gccaggtcct
     8581 tgtggatcac gcgcgtcgcc gcatggcgga gaagcgcgga ggacagtggg aacggacaac
     8641 gctcgccgag ggccggtacg gcatcgcggc gacactggag gacctcgtcg cgctcgatgc
     8701 cgcactcgac cggctggatg cagtcgatcc acgcctgcgg caggtggtgg agcaccgcta
     8761 cttcgcgggt cttaccgacc gcgaaatcgc agaggtgatg ggtattacgg agcgaaccgt
     8821 tcagcgtgat tgggcgcgtg caagggcgtg gctatacaag gagctatatc cggctccata
     8881 gacgacgatg aaaaccgcgg atcgctggaa gaagatcaca gcgctttttg acggagcact
     8941 cgagcaaccc gaagcacgcc gcgcggagtg gctggccact gcctccgacg gagacgagga
     9001 tgcccgggcc gaggtcgaac ggatgctggc cgcgcacggt atcgccggcg ggattctcga
     9061 tcatccgacg ttcaccatgt cacccacgga cctccaggag gacgacggcc cgcaggtggg
     9121 ctccgcggtg gcgggacggt accgaatcct gcgcgaggtc ggtcgtggtg gcatgggcgt
     9181 tgtgtacgag gcccacgacc cgaggctgga tcgccagatc gctctcaagt ttctgccacc
     9241 gcgagccggg actgaagcaa aggcacggtt tctgacggaa gcgaaaacgg cgtccgcgat
     9301 cgaccatccg aacatttgca cggtttacga cgccggcgac acggacgacg gccgcctttg
     9361 gatcgctatg gccttctatg acggtgaaac cgttgcacag cggttgcgta aagggccgtt
     9421 ttcggtggat aagagcatcc gggtcgcgtc tcaggttttg cgtgggctcg agtgcgcgca
     9481 cggagccggg atcgttcacc gcgacattaa gccgtcgaat gtcatgctta cggcgcgggg
     9541 agaggtgaaa attctggatt tcggcgtcgc aaaactggct cacgagagcc cagaaacccg
     9601 gcccggtaca agagtcggca cggcggccta catgtcaccg gagcaagcgc gcggcgaggt
     9661 cgtggatcac cgcacggatc tatggtccct gggcgtggtg atatgcgaga tgctctcagg
     9721 ccagagactg ttccgaggcg aacatgaggt gtcggtgatg cacgcgattc tatacgaaga
     9781 gcctccgacg ccctcacttc ccagcgggct cgaccggatc gttcgaaagg ccctcgctcg
     9841 gcagcgagcc gaccgctacg ccggggccgc agccatgctc gcggatctcg agaggtttct
     9901 tgatccgggg gtaagcccgg cggtgcgcct gccggcgccg ttgacaagct ttatcgggcg
     9961 ggagcgtgag attgcgcaga tcgacggttt actttcgacg gccaggctgg ttacgttgac
    10021 cggacccggc ggcgctggca agacaaggct tgctctcgag gcggcaaccc aactcgccgt
    10081 gaagttcgaa gactccgtgg tgttcgttcc gctggcggcg atcaacgatc ccgacctcat
    10141 cgctcccgcg atggcccagg ttgtgtgctc ccagcggtcc cctggcttgg ggccgatcga
    10201 agagttgcga ttcgctcttc gcgacaggaa gacgctgctg gttctcgaca acttcgagca
    10261 ggttgcggcg gctgcgcccg tggttgccga actgctggcc gcgtgtcccg gggtcaagat
    10321 gatcgtcacg agccgtgtgc ctcttcggct cacctgcgaa cacgaattcc ctgtgccgcc
    10381 cctgcccctt ccacggcgtg gcgacccgcc cgatccgcgg accctggaga gaaacccggc
    10441 cgtcgcgttg tttgtggatc gcgccagggc ggcgcggccg gactttgctc tgaccgcgga
    10501 gaacgccgcc gacatcgccg agctttgcgc aaggctggac ggtctaccgt tggcgatcga
    10561 actggcggcc gctcgcgtga agatgttccc gccgcacgcc atgctggagc gcctgtgcca
    10621 tcgcctggac tttctgaaaa ccgatcagaa agatcgtccg gcgcgccatc agacaattcg
    10681 ccaggctatc tcatacagct acgacctatt gagcgagcac gagcagcgcc tgtttcgccg
    10741 gctggcggtc ttcgtgaacg gcatgacact ggatgcggcc gcggcagtag cgaacgcagg
    10801 cggcccgctg gaactcaacg tcgaagaggg gatctcgggt ctggtcgatc acagcctgtt
    10861 acggcgttcg gacgacgccg gaacagaacc tcgcttccgc atgctcgaga tcgtccgcga
    10921 ttacgcgctc gaacgtctgg aggagagtga gcgggaatac acgagcgagg cgcacgctct
    10981 ccactttctt gcgctcgccg agcgagccga gcctcttctc accggaccga tccaggtgga
    11041 atggatggat cgtctggagg cggagcatga caacctcagg gcggcgatcg catgggcgga
    11101 agagaagggt gaggccgaag caggtctgcg ggtcggcgct gcgctttggc gcttctggct
    11161 cgtccgtggg cacctgaatg aaggccgccg gagactggac caactgctgg cactgccggg
    11221 aaagacggct ccgctggtga gagcccgtgc actgaacggc atgggaacgt tggcgcacaa
    11281 caacggagag aataccgagg cacgagtgtt cctggaagag gcgctcacgc tgttccggca
    11341 ggcaggagac cgaaggggcg tggcctcggt gctgggtaat ctgggctggg tggcttgcga
    11401 actgacggat ctggagacgg ccgaaatcct ttccacggaa gcactggagt tgaaccggga
    11461 cctcggcgag aaacgtgggt acgccctggc gttgaacaat cttggttggg tggccaacta
    11521 ccgctcggag taccgccgcg ccgtggccta ccaggagcaa agccttgcgt tacggcagga
    11581 gatcggcgac agccggggtg tggccttcac gctctccaac atctcgtggg cggaaatgtg
    11641 tctcggcgac tacgtgcggg cggaaacgct tgccgatcgg gcgctcgaaa tgctgcggga
    11701 acttcacgac cggtcgatcc tcggttgggc gctggtcatc cgcgggtgga tcgcttccga
    11761 gaagggcgag cacgagcgcg cggctgcgtt tgtcgaagag tgttacaggc tggcgcggga
    11821 ggccggcaac cggtcgatcc gcgctgccgg gctgaccggg ctaggcatgc ttgccacggt
    11881 ccgcggcgag tacgaccggg cagtcagcct gttcgaagaa gcgatatctc tctggcaagc
    11941 gatcggtggg cgctggggcg tcgccctgtc gctccacggc cgcgcctcgg cgctgcatgg
    12001 cacgggagac acgcggcggg cgttatccga ccttcggacg agcctgcgca tgcggagcga
    12061 aatcggcgac cgccgcggaa tcgccggttg cctggagggg atcgcgggaa tcgaagcgga
    12121 gcagcatcgt ttccgcaatt cggcacggct gctcggaggg gcagcaaagc tacgcgagac
    12181 cgccgtgacg ccggttccgc catacctgag ctctgaactg gagaaggttg ccgcaaaggc
    12241 gcgagggggc ctcggcgtca gggagttcgg cgcagccttg acggaagggc gcgaggcgcc
    12301 tatcgatgcg ctggttcgct cctgcctcga agaggcttga gcgcggagta cactatctgc
    12361 gatgctcatc atcgacactc acgctcacat ctattctccg gacgaaaaga aatacccgcc
    12421 catcgagaaa ccgcttcggc cgccgcgtgg caaagcgtct gtcgaagatc ttcgcaaaca
    12481 gacccagtcg gccggtgttc gcggcgcctg cctgattcag accagcacct tctatagttt
    12541 cgataaccgc tacacctgcg attcggcgaa ggccaatccg gactggacgg ccggcgtctg
    12601 cacgctcgat ccagacaatc cgaaaagccc ggacatcctc cggcaatttg tccgggactt
    12661 cgggatgaaa ggaatgcgca gcatcccggc taccgacggt cggttgaatc acccgagcgt
    12721 cgaggcgctc tggaagacgg cgctggatct cggcatcgtg atcaacattc tgatcgggcg
    12781 gaacaaggcc gacgaggccg atgcgatgct cggtaagttc ccgaagcttc gcgtcgtcct
    12841 cgaccactgc ctgaatccac aggcgggacc cgaactcgac gcgaccttga tggcggtgct
    12901 gcggctggag aggcggaaga acctgcacgc caagctgacg ttcgtgccaa ccggaagtaa
    12961 ggagggctat ccctgccgtg atatgcacga cgcctgcctg aaagtcctgc gggcgtacgg
    13021 cgcggaacgg tgcgtctggg gaagcgattt tccaaatgat ctgtggacgc cgaaggtgag
    13081 cttcgaggag cacctgaaga tcttcacgca cgatcttcct ctgaaacccg cggagcgctc
    13141 cgcagtgctg ggcggcacgg ccaaccgtct gtggttcgga gggaagctcg ggtgaggctt
    13201 caggtcggtt tggcggcgct gctcgtcagc gtaccgctgt tcggcgatcc ttctttgccc
    13261 gcgctgttca gcgaccacat ggtgatgcag cgaggtgttc ccgcgccggt ctgggggtgg
    13321 gccgatccag gggagcggat taccgtgacg atcggcgtgg agagccgcgc cgcggtcgcg
    13381 aacgcacagg gaaaatggga agtgaggctt tctccgctca ccaccggttc tccgctgacg
    13441 atgaccgtgc gtggaaacag gacgctcacg gttcaggaca tcctcgcggg cgaggtttgg
    13501 gtctgctccg gccagtccaa catgcacttc gggatgctcg ccaccacaac agcgaaggag
    13561 gacatcgccg cggcgaacca cccagagata cggctcttca cggtgccgcg tgagagcgcg
    13621 ctcgagcctt tgaaggactt ccgctcggca tggaaagtct gcacgccgga gtcggtcgcg
    13681 gagttcaccg ccgtcggcta tcacttcgga aggaatctcc acgagaggct caacgtgccc
    13741 gtcggtctga ttcagtcaag ttggcctgga actcaggccg aggagtggac cgcacgaaag
    13801 gcactcgatg cggacccgga cttcacgccg attctggacc gctggaatca ggcggggccg
    13861 gagatccata acctctatcg ccggtatcgc cggccggccg atttcgacct cacgtttgac
    13921 gacttcgagt tcgtgccctt cgagggcggt tccggaacgc agccactcga cgattttgac
    13981 ggcggcgccg ggaaccgctt cggcggcgaa tggaccaacc ggtcgaggcc ggaggacaaa
    14041 ttcgcgatgg accgcgtccg gcctgggtac agcgggaatg gggaagcact gcgcttcagc
    14101 ggaccgcaga gggcaccgga cacggtgcag atccactccg ccttcggtgc ggccggtgag
    14161 tcgcgagaca tgagcaagtt ctccggtctc cggttcttcg tgcgagggaa gggtttcttc
    14221 aggattcatg tccttcagca aagcgtccgc gattccgaca actacacctc ggctatcctc
    14281 ggcgcttccg gcgattggac accagtgacg gttcgttttt ccgagttgaa acgcggcggc
    14341 tggggacgcc cggcttcgtt cgctcccgag gagctgacag gcatccttat cgaagcaata
    14401 cccgctgcga tcacgccgcc ccggcctccg tccggtctgt tcaacgcgat gatcgcacct
    14461 cttgccccgt tcgcgattcg gggtgcggca tggtatcagg gggagggcaa tgccggaagg
    14521 gcgtatcaat accggaagct gctgcccgcg atgatccact cgtggcgcga ggcatgggga
    14581 cagggcgact ttccgtttct gatcgtgcaa ctgccgaact tccggcagcg taaagccgag
    14641 ccgggtgaag gcaactgggc cgaactgcgg gaggcgcagt ttctgacgta caagacggtt
    14701 ccgaacaccg gcctcgcggt gacgatcgag ctgggtgagg ccgatgacgt ccacccacgc
    14761 gacaagcggg aggttggacg gcggctggct ctggcggcgc tgggagccgt ctaccggcag
    14821 gaacgcgtgt actccgggcc gatccaggac tcggtgaaag tggaggggag ccgtgtgcgg
    14881 gtgcggttcc gtaataccgg cggcgggctg attgcgaagg atggcgggcc gctcaaggga
    14941 ttcgcgctcg caggagagga caggaagttc gtctgggcgc aagcgaagat cgaaggccac
    15001 tccgtcgtgg tgtggagcga ttccgttccg aggcccgcag ctgtgcggta cgcctgggcg
    15061 gacaaccccg agtgcaacct gtataacagc gacggtctgc cggctccgcc attccgcacc
    15121 gatgactggc cggggctgac tcgcgaagcg cggtagcttt cccagttctt actgctcgaa
    15181 gacgacgtcc acaggaatgc ccgcgccgtc gagccgcata aggtccgatt tcagcgtgtc
    15241 acttgacgtg cccatttccg cgaagaagcg gccgacgcca tcgtagtcgc cgtccccttg
    15301 caggcggatg atcttttccg aaagactgtc gacggccgcc tgcatcttct cctcattgac
    15361 gcgatacgtg ccggatgcgg cacctcgggt gaaggcgccc gcccgctcga agtagttgaa
    15421 ccggaccaga ttcgccgcgc cgtgggcgct ggttccaccg aagcggatgg agcggaagat
    15481 tccggcgagg aaggtaacgt agtagtccat gagttccgct ttgccgagtt cctctttctt
    15541 gtgaagctcc gagatcatgt acaacccgag gatgtccgcc ttgccctctt cgagcgagga
    15601 ggcgtgctcc ttcatgactt cgcgcaccat gcccttgccg tcgatggttt tcttgatgcc
    15661 aaggccgtgc gccacctcat ggaacatggt ttccgcgaag aacggatcga agccgacgtg
    15721 tttacgctgg tccgcggcga tgagcacgcc ggcgatcggg agcagaatct tgtcgaactt
    15781 ggctcgcatc gcgttcttca gctggagccg ccgcgtgccc ttcttcaact gcacctcttc
    15841 gtcgttgggc aggttaatgg cgatggtctt cgaaccggcg ttgcagtgtc cggcgtagta
    15901 gattgcgtcg taggcgttga ggtccgagtc gaggcccggc ttctcctgct tgtaggcgtc
    15961 cgggaccggt aggccctttt ggagcgccgg cagcatcgac gtatacttcg ccaggcgccg
    16021 gctccactcc ttgtccttca caagcacgta ggcttcgtag gccgccttgt agccaaacag
    16081 ccgatcctcg tacgtctcga tcggcccgat gacgatgtcg acggtgttgt ctttcatgtc
    16141 catccaggcc agatcgctgg ccatgtagtc gtcggtaagc aaggcgtcgg cgcggagttc
    16201 gagatacttc cgcaatcccg gactggaggc gagtttcgcc gcctcccgga gttttgcagc
    16261 agcgacttga agctgagtcc catattcggt gtgatacggc cgcgcttcaa gagaccggtc
    16321 cgccttgcgc cgcacaagcg tgtagaggct cttgagctgc ttgcctccgt cgggagccac
    16381 ggcgacggca gcctcgaact cctgcttcgt catgtccgcc ggatagagat ttgcgccgag
    16441 cggcttcggg ccgacgccgg atacgaacgg cgcctcatcg tcgagccggt cccagggacc
    16501 gtagttgatc tcggcgaacc gccggacgtc cgggccggcc agacccgaga gcagagtgtt
    16561 cttgtccccg tacgcctgga gccagaacag gtcgtccatg accctggcgg cctcgatcag
    16621 gagcgggatc atgcgccgct cgttttccgt gagcgagctg aggtcggttt ccaggcggaa
    16681 agagacgtac ttggaaagtc gttgctttgc gggcatggct tcagccttag gggtcgtttc
    16741 tttccggcaa gcggtcaaaa gcaggccggt ggcgaagagc agggaaatgg cgaatctgga
    16801 tcgcatcgga gtatgatgac agcctcgtcc gattgtatcc tccgccggag aaaccaggga
    16861 accaagatga acgaaatcaa gctgatcacg ctcaaccccg ggcatttcca tgccgctctt
    16921 gtccagaagg agatgtatcc ggatgtctcc cggcaggccg ccgtatacgg gccgctcggg
    16981 ccagatctgc tggagcacct cggccgcgtc gtgcgtttca acgttcgtga agagagtccg
    17041 acggcgtggg agttagacgt gcgttgctcc ccgcgttatc tggacaggat gcttgcggac
    17101 cggccaggca acgtcgtcgt gatcgccgga cggaaccgag ccaaaatcga cctgatccac
    17161 gcgtcagtgc aagccggctt ccacgtgctt gccgacaagc cctggatcat cgagacagcc
    17221 gatttgccga aacttgagga aacgctcgag acggccgaac gcaaaggcct ggcggcctac
    17281 gacattatga ccgagcgtta cgagatcacc tcgatcgtac agcgggcgct ggtcaacgac
    17341 gatgccgtct tcggagagat ggtcgaaggc agcgaggacg ccccagcggt gctcatggag
    17401 agcgttcacc acctgatgaa gaccgtggcc ggtgtgccgc tgcgccggcc accctggttt
    17461 ttcgacatcc gcgagcaggg tgaaggactc accgacgtgg gaccgcatct agtcgatctc
    17521 gtgcagtggg tcctgtttcc ggaacaggcg atcgatcatc gcatcggcgt ccgcgtcctc
    17581 aatggccggc ggtggccgac cgtgttaagc ctcgaggagt accagcgagt caccggagag
    17641 cggcgtttcc cggacgtgcc ggaggcgagc ggcgcaaaga tcgagtactt ctgcaacaac
    17701 tcggtgttgt acaggctgcg cggcgtccac gtgaaactca ccgcggtctg gaactacgag
    17761 gcgccgccgg gcgcgggaga cacgcactgt gccctatttc ggggtacaaa ggcacgggtg
    17821 gagatccggc agggagcgga agagggctac cgtccggagc tgtacgttgt gccggaacag
    17881 ccgggcgagg cagtcggcag cgcgctccgg agcagagtcg atagcctagc gtccgagtat
    17941 gcggggatcg cccttgaaca ccagggcgcg cgataccaca tcgtggttcc agaggtgcac
    18001 cgcgtggggc acgaggcgca tttcgcgcag gtggccaggc agttcttccg tttcgtgcgg
    18061 ggtgaaggtg gtttgcctgc gtgtgagaag ccgaacatgc tgaccaagta cttcgtgacg
    18121 actcaaggcg tcgcgttgag ccgattgtaa gtaatttaaa atgagataga tagtgtgtgt
    18181 agggaggcat cttgacgtcc atgctgaata atgatatgct cataatagtt gatagggacg
    18241 cgctaaagta tggacatcaa ccgctttacc gagaaggcac agcaggcgct gcaagcggct
    18301 cagagcaagg ccgtccgctt cagccatcag caaattgacg tcgaacatct tctagccgct
    18361 cttctggagc aggagggcgg gttggctccc gccattctaa acaaggccga tgttcgtaca
    18421 gagggactcc gccagcggct cgagcaggaa ctggaccggt tgccgaaggt ctcgggatca
    18481 tcgggaacac cggaccagat ctatgtcacc gggcggctga atcgcgttct gacccaggcg
    18541 gaagacgaag cgcgccgcct gaaggacgag tacgtttcca tcgagcacat tctgctcgcc
    18601 atgacggacg acggcggcgt gacaggccgc ctcttcaagg aatttggact gacgcgggat
    18661 cgcttgatga aagccctcca ggaggttcgc ggcaaccagc gcgtaacgtc gcagactccc
    18721 gaaggcacct accaggcgtt ggagcggtac ggccgcgacc tgacggcgct ggccggccag
    18781 ggcaagctcg atccggtgat cgggcgcgat gacgagatcc gccgggtgat ccaggttctt
    18841 tcccgccgca ccaagaacaa tcccgtgctg atcggtgaac ctggcgtggg caagacggcc
    18901 atcgtcgaag ggctcgccca gcgaatcgtg cgcggggacg tgccggaggg ccttaagaac
    18961 aagcgcgtcg tcgcgctaga catgggcgcg ctgatcgccg gtgcgaaata ccgcggggag
    19021 ttcgaggaac gactgaaggc cgtgctcaaa gaggtccagt cctcggaggg cgaaatcgtc
    19081 ctgttcatag acgagcttca caacgtcgtt ggggcaggga aggcggaagg ggccatggac
    19141 gccgccaatc ttcttaagcc gatgctggcc cgcggagagt tgcactgcat cggcgccacc
    19201 acgctcgacg agtaccggaa acacattgag aaggatgccg ccctcgaacg gcgcttccag
    19261 ccggtgctgg tggatcagcc gtcagtggag gacactatct cgattctgcg cgggctgaag
    19321 gagcgctacg aagtccacca cggcgtcaga atcaaggaca cggcactggt cgcggcggcg
    19381 gtgctctcca accgttatat caccgaccgg ttcctgccgg acaaggcgat cgacctggtg
    19441 gacgaggcgg cagcaaggct gcgcaccgag atcgattcga tgcccgccga acttgacgag
    19501 cggcttcggc gcatcatgca actcgaaatc gagcgtgagg cgctgaagaa ggagaccgac
    19561 gccgcttcac gggagcggct ggcgcggatt gaaaaggagc tggccgatct gaagtcggag
    19621 gccgacgcgc tcaaggccca gtggcaggcc gaaaaggacg cggtgcaacg cctgcagtcg
    19681 ctccgcggac agctcgaaca gacgcgtcac gagatcgaac tggccgaacg gcaatacgat
    19741 ctgaacaagg cggccgaact gaagtacggc aagcttaccg gcctggagcg acagatcaag
    19801 caggagcagg cggcgctgga ggacaaacaa gggcagatgc agttgatcaa ggaagaggtc
    19861 gatgagcagg acatcgccga ggtggtcagc cgttggaccc acattccggt gtcacggctg
    19921 ctcgaaagcg agattcagaa gcttcttcat ctggacgagg agcttcacaa gcgggtgatc
    19981 ggtcaggacg aggcggtgca ggcagtgtct gaagcggtgc tgcgcgcgcg ctccggcctg
    20041 aaggatccga accggccgat cggcagtttc atctttctgg gaccgaccgg agtcggcaag
    20101 accgaactcg cgcgggcgtt ggcggagttt ctttttgacg acgagcgggc gatgatccgc
    20161 atcgatatga gtgagtacca ggagaagcac accgttgccc ggctgatcgg cgcgcctccg
    20221 ggctatgtgg gctacgagga gggaggccag ctcaccgaag cggtacggcg gcggccgtat
    20281 tccgtcatcc tgttcgacga gatcgagaag gcgcaccacg acgtcttcaa tgtgatgctc
    20341 cagatcctcg acgacggccg gctgacggac gggcagggcc gtaccgtgga cttcaagaat
    20401 acgatcgtaa cgatgacgtc gaacgtcggc agccaccgca tcctaggcta ccggggtgcg
    20461 ttcgagggca cagagtacga gaggatgaag gaagccgtgc tcgacgagat gcgacgttca
    20521 ttccggccgg agttcttgaa ccgtgtcgac gaggtgatcg ttttccacgc gttgagcgag
    20581 gaacacctgg cggcgatcgt gagcatccag ctcgagcggc tgaagccgcg gctggccgag
    20641 cgccatctgg aactggaagt gactgatgcg gcgcgtcgcc atctcgtccg ggccggctat
    20701 gacccgaact acggcgcccg gccattgaag cgggccattc aaaaggaaat cgagacgccg
    20761 ctggcgcggc tgatcttgaa aggagacgtt cgcgagggtc agaccgtggt tgctgacctg
    20821 gacacggagc gcggagagtt gaaattcacg ccgcggactg tcccctccgg tgagaagaag
    20881 agcgccggcg cgagctgaga aatgagcaaa aagccgaggc atccgtcttc cttcatgctg
    20941 ccgattgccg ggccgaacag cgacccggta tggcaacccg tagccgacgt ctatcggact
    21001 ctgaccggct ggctggtgaa gttcgagctg gccggcgtgg atcccggcga tgtcgagatc
    21061 agcgtcgaaa atacccggct caccgtcact ggaatgcgcc gcgactggac gttcgaggag
    21121 gtttgcagcc actactcgat ggaaattgcc tataaccgtt ttgaacggtc tctggaactg
    21181 ccctgcaatc tggagcgagc acacctggat ctggagtacc ggaacggaat cctgctggtg
    21241 cgcgtcacca caaaggggga gtgaatcgtg cacgatcaca cgaaagtact gccgctcttg
    21301 ccgctgaaaa acagcgtgct gttcccacat ctgttgatgc ctttgtcagt cggccggccg
    21361 acgtcattgg cagcggtgga cgcggcgttg tcgacggaag agaaggaaat cgtcatcgcc
    21421 gcccagcggg acgccgccgt cgaggtccct ggaatggccg atctttattc cgtcggcacc
    21481 cgggcggtga tcaagcggat gtcccgcccg agcgagagtg cgatggagct gatcgttctc
    21541 ggtacggaac gggtgatgct cctcaggctg gactcgaccg atccatacct gaaggtcgag
    21601 gtgcagcctc ttgctctgcc cgccgaagaa agccccgagg tggaagcttt gcatcacgag
    21661 gtgatggaac tggccgccaa agcgattggg ctggcgcagc cccaggcgcc cgccgagatg
    21721 agccgggtga tgatgagcac cgaagacccg ctccggcttt gctaccttct ggcctcgatg
    21781 ttcagcctgg agattgacaa ggagcaggcc ttgctcgaag ccgcgactcg ggctgacgcg
    21841 cttcggctga cgcactcgtt tctcgctcac gagattcagg tgctcgagat ccggaacaag
    21901 atcgcgagta ccgcccagag cgagatgggc aaggagcaac gggagtactt gctccggcag
    21961 caactgcgcg ccatccagca ggaactcggc gagaagaatc cggaacaggc cgaggtggac
    22021 atgctgaagg agcgcctgga aaaggccgac cttccggacg aaatccgtaa ggagacggag
    22081 cgtgagcttt cacggctgga gaagctgccg tcggcctcgc cggagcacca tgtcattcgc
    22141 acctatctcg agttcgtgct agagctgccg tggcgcgtcc acacggacga tcagttggac
    22201 atcgcccagg cgcggaaggt gctcgatgaa gaccactaca acctgaaaga ggtcaaggag
    22261 cggatcctcg agcaactcgg cgtgctgaag ctcaacccgc aggctaaggc gccgatcctc
    22321 tgtttcgtcg gcccgccggg aaccggcaag acctctctcg gacagtcgat tgcgcgcgcc
    22381 atcgggcgga agttcgaacg catgagcctc ggcggcatgc acgacgaggc ggagctgcgc
    22441 ggccatcgca gaacgtacat cggagcgatg ccggggcgtc tgatccaggc gatccgccgc
    22501 gccggcgcca acaaccctgt gctgatgctc gatgaagtcg acaaactcgg ccgcgacttt
    22561 cggggcgacc ccgcggcggc actgctcgag gtgctggatc cggagcagaa caagacgttc
    22621 cgcgacaact acctcgactt accgttcgac ctctcgaagg tcctcttcat cacgacggcc
    22681 aacacgcttg ataccatccc gcggccgctg ctcgaccgca tggaaatgct tcgtctcccc
    22741 ggctacagcg aggaggagaa gctcatcatc gcccggaggt acctcattcc gaggcagctc
    22801 aaggaaaccg ggttgaccgc ggaacagtgc gtaatcggcg atgacgtggt gagccgtatc
    22861 atcaccggct acaccaggga agccggcctg cgccaattgg aacgggggat cggtcgggtt
    22921 gctcgtaaag tcgcgctgcg ctttgccgaa ggtccgtccg aaccagtcac cgtgcatccc
    22981 gacgagcttg ccgacatgct cggtccggag cgtttcttcc cggagaaaat gcgccgtgat
    23041 cttgcgcccg gcgtggctac cggacttgcc tggaccgaga ccggcggcga cgttctttac
    23101 gtcgaagcga cgctgctgcc agaaagcaaa gggctgactc tcactggaca actcggcgac
    23161 gtgatgcagg agtcggccaa gaccgcacag agctacatct ggtcgcatgc cgaagcactg
    23221 ggtatcgatc gcgcactgct caaagacgcc ggcgtgcaca tccacgtccc ggcgggcgcg
    23281 attccgaaag acggtccgtc ggctggaatc acgatggctg tcacgctggc gtcgctttat
    23341 accaaacagc cggtgcgaag cgacacagcg atgaccggcg aggtgacgct taccggactg
    23401 gtgctgccga tcggcggcct caaggagaag gttctggcgg cgcggcgcgc tggaatcaag
    23461 cgcgtgatcg tgccgaagcc gaaccagacc gacttgcgcg agctacccga ggaagtccgc
    23521 tcggagatcg agttcatctt cgtggagcgg gtggaggaag tcatctccgc ggccgtgccc
    23581 gcgctggcgg aacggctgtc tccggtgaag gtcggctgaa cggggctctg tgtgaggcgg
    23641 gcgatggccc gcctcacact acttttctgt cagcgccgcc acgccgggaa gatcgatgcc
    23701 ggagaggaac tcgagagagg ctccaccgcc ggtcgagatg tgcgtgatct tgtcggccac
    23761 gccggcggac ttgattgctt tctccgaatc gccgccgcca accaccgata ctgcgcccga
    23821 ctcggccaca gctttcgcca gcgccatggt gcctttgtca aacggcggtt tttcgaaaac
    23881 gcccatcggt ccgttccaga tcaccgtctt cgaagcggcg atctccttcg cgtaggcatt
    23941 gatggtcttc ggaccgatgt ccagtcccat cttgtcgtca gggatcgtct cgactacctc
    24001 gttcgcagcg ccttccttca actcggctgc caccacgtgg tccagcggaa gtaacagctt
    24061 cccgccggcg gccgcgagca gttccttcgc cagatcgacc ttctccgcct cacccagcga
    24121 cttgccagtc ggctgtccct gcgccttcag gaacgtgtag gccatcgctc cgccgatcag
    24181 gagccggtcg acgaacttca tcaggttctg gatgacctcg atcttgtccg acaccttcgc
    24241 gccgcccagg atcgcgatgc acggccgctc cgggttcttc gtggccttcg tcagccattc
    24301 aagttccttg tccatcagca ggccggccgc tgccttagac acgaacttgg tcattccttc
    24361 cgtggaggcg tgcgcgcggt gcgccgagcc gaacgcatcg ttcacataga catcgcatag
    24421 cgacgcgagt ttcctggcga actcgggttc gttcttctcc tcctcggagt ggaagcggag
    24481 gttctcgagc aacagcacct ctccgggggc cggtttcatc gcttcgacct cgggtccgac
    24541 acagtccggg gccatcttga ccgggcgccc gagcagttct tcgagccgtt tggccaccgg
    24601 cttcaggctc atctcggggt tcggcttgcc cttcggccgt ccgaggtggc tcgccagaat
    24661 caggcccgct ccctgttcga gggcgtactt gattgacggt agcgaggcct tgatgcgctt
    24721 gtcgctggag atctcctgcc cgcccggggc aagaggaacg ttgaagtcta cacgaatgaa
    24781 gacacgcttg cctttgagat ccagatcacg aatcgaaagt tttgccatat cagtctctta
    24841 aatgtctgat cgtaacacgt cgtatatgat gcgaatccct ttgtagaact cgctcagagg
    24901 gattcgttcg gcgtcgctgt gcatggtggc cgaggtggcc atgtcgatca ccatcggcgt
    24961 gaatccatat gccgtgatgc ctttgacgcg cagttttact gagtcggtcc cgtgaggcac
    25021 cagcatcggc gtcacgatcg cgccgggatg gtgtttggcg atggattccc ggatcgccgc
    25081 gaacagcgga gtgtcgctcg ggctcacgcc cgggtcgttc ggttcgctgg caagctcgat
    25141 ggtcactcgc gggtcgttga tccgcgcttt catctccgaa atgaactcct cggcgtcgac
    25201 gccgggcagc agccggcagt cgagcgtcgc ctcgcttgtg gacggaatca cgttgatctt
    25261 cggcggggac cctacgccgg ctgagagcgt ggtcagcgag atcgtgttgt cctggatggc
    25321 gttggtgtac ttgttcttcg ccagcgccgc accgatgttg cggatcatct gctccactac
    25381 cgggtgaggc ttcgccggcg ggcgttccat cgcccgttcg agcgcgcgaa gcagcgtcat
    25441 gttcgcgtta tccggaatcg gctgcgaccc gtgggcagcg gtgccctttg ctctcagccg
    25501 cagccatgtc gtctgcttct cgcccaccgc gattccgaaa accagcttgt tctccgccag
    25561 aatgtcccga gtgccgaacc cgccctcgtc gagcacgtac tcggcgttga tctcgtcgaa
    25621 gtggttcttg atcatccaga ggatgccgta tgtgccgcct gtctcctcgt cggcggtaga
    25681 aagcatgacg atgtcgcgcg cgggaacgat tccggactgt ttgagcgcga ccaacgccgt
    25741 cagatgctgg atgccgatgc ccttcatatc catggcgccg cgtccccaga tccagccgtc
    25801 tttggcgatg ccgccgaagg ggtctatgtt ccaggcgctc cggtccactg ggaccacgtc
    25861 gagatgattc agcaggagca gcggtttttt cgagcggtcc ttcccgggca ggcgtgccag
    25921 gaggttggtc tggccgcctg gcccgctggt gtacagcttc acgtcgatgc cgtgcttttc
    25981 gagttctgtc ttcagcaggt tcgccgcctc ggccgtgttc gccggcgggt tgatcgattc
    26041 gatgcggatg taacgttgaa gcaggtccag cgcatatggc tcgagcttcg cccaatcggg
    26101 ttccgccgcg aacaagctac acgccacaag gctcatgcca agcagtttca tttttcggcc
    26161 tccggaagat tagtgatttt cggcgccagg ccggcgtttc gatacgcctc gatcagcgct
    26221 ccggccgcgg gtccgtagac tggcgggccg caacgattgc cctcctcgag ttccacgagc
    26281 atgcggaaac gctcgagcag gatgccgctt cggaacacgc cgccgatata ggagacccgc
    26341 gcagattcgt tcctgtcgaa gagctgaccg cggacggcgg cggcgattga cgccagttcc
    26401 tgcgccgcat tctcgagcag ccggccggcg accgcgtcgc cctccacggc tgcttggtct
    26461 accagggccg cgtaggaggc gatgtgaggg cgcgagtact cggtcgtgta gaaacggtgc
    26521 aacaggtcgt tggcgtccgg cgcgcccgtt gcttcgagaa ggacgcggcg gagcacggtc
    26581 ggcggtcccc agccctcttc gtaacgcagc gccgcacgaa gcgattgccg cacaacatcg
    26641 aacgctccgc cctcgtcgcc gaacacgtat ccccaacctc ctgcgcgggc ggtcttgccc
    26701 gccgtattgc gaccgaacgc aatcgaaccg gtaccagcga tggtgatgat ccccggttca
    26761 cccgcagtcg cgccggacag cgcaatcagc gcatcggtag tgacggccag tcgctccgtg
    26821 ggaaggatct cttcgagaat tgcctgctta tccgccggac caccgctaaa gccgagacac
    26881 gccgcggcga aacggaagct gctctgctcg agaccggcct gagcgcacgc ctttccgaca
    26941 cactcgcgga tgacccgtgt gaacttggcc cgtccttcgg cggcgccaac atggttgcac
    27001 ggcccacccc tgcctaaccc gagcacgcgc ccgttctcgt cgccgaccat cgccgtcgtg
    27061 cttgattgcc cgccgtcaac ccccaagaag tacgaagcca cctcaacatg gtactgaagg
    27121 tgcgtgtctg cggagggggc tgaaccgcat ctctcagagc cggcttggta cgagctccat
    27181 ttccggcacg acggcccttg gatggagccg tgccaccgcc accaccgcgt ccgccacgtc
    27241 ttcgggttcg agcgcgagat ccagcatctc cttgggtgtc ggcgacggcc gcttgtcgag
    27301 gatctccgtc aggcagagtc cgggaaaaat aacgctggtg cgaatgccgt tcgctttctc
    27361 ctcgacgcgg gttgcgtgcg ccagtccgga gattccgcac ttactcgcct ggtatgcggc
    27421 gcccgacacg tccggcacgt gagccgagat cgtcgagatg taaatgatca gcccgccgcc
    27481 tgcatcccgc attaccggca agaccgcgtg ggtgcaatgg aacgccccgg tcaggtttgt
    27541 ggcgatcatc atgtcccacg tttcggcggt cagcagcttc atggaccgag cgggaatgtt
    27601 cgttcccgtc gcgaagacta ggatgtcgag ctgtccgaac cgcttcagcg tcgccgcaat
    27661 gagcgcctcc acttgcgccc ggtcgactac atcgcaaggg catgtgtcga gcacaccacc
    27721 ctcacgtcga gcctcctccg ggagcccagc gagcctgtcc tcgcggcgcg ccgccgccat
    27781 tacgcgagcg ccatagcgcg ccagtgccag ggcggtggcc cgcccaattc cgcttgacgc
    27841 ccccatgact accgcaactt tgtctcgaat cgaaatagcc accctgtcgt ctcccgcccg
    27901 ctttttgcag tgtgccgcaa ttgccgttca cttcgttaac atcaccagat gtccttcgcg
    27961 aatccctccc acaccagggc cgggaactgc cgtatcgtcg caaatcctac acgttcgtac
    28021 aggcgcacgg ctccagtgtt cgccgccgtc acggtcaggc ttgccttccg gcacccggaa
    28081 gccgccagca cagccaggga acgccgcaag agttcatagc cagcaccgat gccctgcatc
    28141 ggcggggaca cgcagatctg cgtgatatgt cccacgtcgc gcgccagcag gctcgccaga
    28201 cacacaccgc atagccgccc tgtcgacacg tccgaagcca gcaaagacgc cggctggaag
    28261 aactggccgc atccagggta ttgcacgatg ttcgtcagga accgtcttgc cccgccggcg
    28321 gaccggtact ggtcgttgat cccgctgtcc acgtggcccg agtacgcctc ggcaatgacg
    28381 cgcccggctt cctcctgccg gccgtcactc cacgtttcaa aatgcaggcg cgccgccgcc
    28441 gcacccggag acagttcgct ggtgcggcac aggtcgaaaa ccatgaaatt ccttggatgc
    28501 acggaaagaa actcgccctt cggaagattg cggccattcg gtacgaccat catcatgagc
    28561 tgagcttcga cgcgtctcag ataaggtatg gccagcagat cgttgagaac cgcttcgagc
    28621 agtgcgttct cattgcgggg cgtgcgatgc tcgtggagga cgtataagtc tccgaccagc
    28681 ccttttcgct cctcggtcac aaagtaggag tagccggcca cacgccggcc tgcgagcaac
    28741 gcatatccgc tcagtgcctg catgcccacg aaccgtcgaa ccagttcggc cgaggggcgg
    28801 aaatcccagt cgaggccacg tcgccactcg cgaagctcat gttccagcag gtgcgacaga
    28861 tcagccgcgc tgatctttct caggtccacg agttcaagtg gctgcgcctc gctgagtgcg
    28921 gccataggcg aattatatgc tggcgccggc cggaagcgcc ggttcggcgc ggacggatca
    28981 ccgtttcagt ttggcgaggt gcgcgccggc cgcgcggtcg atccacgtag tcgggtagtc
    29041 ggcgcgtagc ttttcaaacg cgcggatggc ctcctcggtt cgacccgccc ggagcagggc
    29101 ttcggctttg gaaaaccggt cgctgccgac ggtcatggcg ccttcggcaa tgcgaagcgc
    29161 ttcgctgggt gtgatggtct ccgggacctt cttcagcgcc ggcggcgccg ggtccgtcag
    29221 gatgccgtag gcggcgggag tgcgctcacg aaacagacgg gcgcgcatgt cagtctgcac
    29281 gttccaggca tcgccggctc gccggcccgc gaatggattc acgtcggcgg ttacgatgtc
    29341 gcccggcgct ttcccttgcg cgagcacctt gccctggggc gagatgacca tcgcgcctcc
    29401 ccctcgcttg gcgacgacga tgtagacgaa gttgtcggcg gcgcgagtcc tgaaagacgc
    29461 gcgattcaga ctttcgtcgt ccgaatccgg gtcgccggcc gtcgtcgcgc cgcctagagt
    29521 gacaagaaag atcacgtccg caccggccat cgccaggctg cgggacgact ccggcatgac
    29581 catgtcgtag cagatcaaca tgccgacgcc gccaaggtcg ggggtttcaa aaacgggaaa
    29641 gctggtgccg cgctcatgat cggtttcgct gatggccgga tgcaccttct ggtaccgccc
    29701 gatctccttg gcgtcacggc cgagaaagaa ggccatgttg cgatactttc cgtcgggctc
    29761 gaccgtgtcg gatgagcaga ccaggtacat gcgatgagcg gcggcggcac ggccaaggcg
    29821 gtccagcatc cgggcgacag cggcgggcag cacctcccgt agcaccttct tgttgcccgc
    29881 ctcccagcgg accagcccga gagtgtcctc cggcagcgcg aacgcgtcgc aaccggcggt
    29941 gccagcccga tgtaccgtct tctccagttc ggcaagagtc cggtcgactt cggcaagggc
    30001 ctcggagggg ctgcgtttgt agtcgatcag ccgggacggg ggctgggcgg tgcagagcct
    30061 gacggttgat cccggtcgcg ctggctcggc agcatgaaga gtgattgccg agacgcatgc
    30121 caccagtgac acggcggaga gagccttgcg cgtcatcatc ctgaattcct cctcgctgta
    30181 agagattgta cttcgccttt ccggcgagcc atgcgcgttc gataaaattg caagtttatg
    30241 agttggcgaa cgggaatcgt ccttttggcc gcggtgccgc tgttggcggg cttgcggccg
    30301 catccgcggg tggaggtgga tgcagagctc atcgcaaagg tgcgggagtt gaggaaggca
    30361 ggcgatctgg cgtgggcgag gttcgaccgc tggacgaata cgagtcctga gaaagactcc
    30421 gacaccaatc gcatcgtcgc gtgcatgttc cactacctgg tcagcaacga agaggggaag
    30481 ttcaactgtg cctggcagcc agtgcaaagc aaggtctatc gaaacgggac ggacaggagc
    30541 ggcgggctga tgccgctgct cgatctgtac aacggtgaca agcacacggc ggcttaccag
    30601 ggcggtgtgc tcgtggcgat catcgcgagg gtctacgact ggggctatgg aaggctttca
    30661 cccgagcagc gacaggatgt agtcgagtgg ctgaacgacg ccgttgcgtt tctgcacctt
    30721 aataacgacg atgggttgct ctatctgcgc aacgatggtg cgtcggtgac acagggagta
    30781 gctgcggcta cgtatgcgac gctcggcgag aaccccaagg gcgaacaact gatggcgtgg
    30841 ttccgcgaac gctggcgcga gacgctgaaa gggctggaca tcatggggaa aggcggcgca
    30901 accggcgaag gcaacgcgta cggcacgtcc ccgaccgggt ccggcatcat cacggcggcg
    30961 aacgttgctt acacagcggc gggagaggac ctctttctaa gccacgtgtg gttccggcaa
    31021 cggctgctct acgatgcgtt cgcggcgtat ccggggacgc tgggaggccc ggggatgccg
    31081 accggcgtgc cgcgggaacc gatgatcgag caggcgtcca tcgccggcga cgggcgaagg
    31141 gctgtgtcgt ggcacaaccg cgatactcga cgtaacggat tagtgctggc acggcggttc
    31201 gccgggaccg aggaagccga cacgtggaac tgggtgttcc ggcaaccaga ggtagaccac
    31261 ccgaccgacg actcgcaagc cgtctatgaa ctggtgtact actcgccgcg gccgcggctg
    31321 gtgaaaccga agcggctgtc gttcttcgat ccgagcatgg ggtttgtgta cattcggtcc
    31381 gactgggact cgcccgacgc cacctggatc tcgttctggg ccggcccgca catcgacacg
    31441 catcagcacc tcgatcaggg tacgttcacg gtgttcaaac ggcgcgatct ggcgccgaag
    31501 accgggcatt acgattcgga cgttttctac ccgcatcatt tggcgtacta catccgcacc
    31561 gtgagctcga atggaatcct ggttggagac ccgaaggaga ttttccgagg cttcatcgcg
    31621 gggatgggct gcgacgaaaa cggaaatacg ctcaggacga tgacgaaggc aacgtggccg
    31681 gcctgcatcc cgaacgacgg cgggcagcgg acgatggccc cgcagagtct ggcggtggcg
    31741 aatgcggcgc agttcgagcg gtaccgtgat attttcgacg tcgcccgagt ggtgtcattc
    31801 aaagatgacg ggcgggcggt gttcgtggtc gccgacatta cgaacgctta caacaacccg
    31861 cgtcacacga ctccgggaaa ccgccccaag gtgaaccgcg tgtaccggag agtggtgtac
    31921 atccgtggcc tcgatgttct cgccgtggca gacacggtgg agtcgacgga ggcgaagttc
    31981 gagaagaaat ggctgctgca cgcgctagaa cgtattgaaa tcggcggcga ggtgcagaag
    32041 gtcggcgatg gtgagacagt gcaccaggag acggacgaag cgaagatcgt cgtggacgac
    32101 acgagcccgt cggatcggga acagaccacc tttgacttga ggaggggtta cgcggcgctg
    32161 aacgttaaaa ccctgtttcc tgtgaagttc cggtaccgca aggtcggggg aagggaaccg
    32221 gcggagaccc cgcacgcgga cctttatacc cccggacgca acgcgcggca cttgcaccgg
    32281 cacgtgaaag acttctgggt caaagacttc aatgagggag tcattccgaa ccacaaatca
    32341 gtcaactggg cgccggagcg gccactcgaa gcagcgcaga gcgaacatgt gccggttttt
    32401 ggtccaggat acggacgatg gcgtctggaa gtggaacccg acgcgcccgg caagacggac
    32461 tatttcctga acatcctgaa gccggcgctc aatccgactg aaaaaatgcc gaacacgcga
    32521 cggatcgaga gcgacagcac gtttggcgcg gagatcgtca aggatgggac gaagtacacg
    32581 ctggtgttca gcaaggatgg gttggaagcg ccgaagctgg agatccaagg cggtaactag
    32641 aggatcggct cgccggttca gacgtttcga atgagagcga ggtcgcctgt tttggcggcc
    32701 tgaagcaccg gacccatgta ggcgtcgacc atctccggtg tcagcggtac cggcatgttt
    32761 tcgagcttca tcctgagctg cggatttttt gcggcggtga gtgctcgttc gacatgcgca
    32821 tcggtgaagc cggggatctc gctgagcgtc gtgggcacgc cgatctttct ctcgaacccg
    32881 atcagcgcgt tggcgacggc ttctccaagc acgcggcctt caaggttctc gacgcccagc
    32941 gctgcgtaac cgaggtcccg gcagattccc gccaccaatt ttagtggctc ttcgaccgcg
    33001 ggagcgaaaa ataccgcgta gtacgggttc agcatcccgc atgcccggcc atggctgaga
    33061 atgtccacca gcgagaagct ggtcaggtgc gcaccgttcg tgccgccgac catgatggcg
    33121 tagccgccga gatcggtggc tagtccaagc gcttcgcggc cttcgcgatc ctcggagtgc
    33181 gtcaggacct tcggcaggta ctccaggaca agactaatgc cggcgcgtgc aactcgttcc
    33241 aagagcccgt agttcggctt cccgacggcg ctgaagagga cctcgagaga gtgcgcgatg
    33301 ccatcgagtc ctccgtcagt cgtcagcgcg cgcggcatcc cggttgtgac cgcgtagtcg
    33361 aaaagggcac ggctcggcac gatggcctcg tccacgatca atttcttctg gccagtggcg
    33421 atatcggtaa tgttcgagta cttggtgagg tgtgcgctgg agctcgatac ggtctgcacg
    33481 gcgacgtgtg gcgtaagccg cctgccggag gctacgagag ttttggagac gagaccagtg
    33541 ccgaagtagt cgtcgatcga gccgccgagc gcgcgaagta cctccgcggc ctttgccgca
    33601 tcaatcgtac taccgccgcc aaagctgacg atgacgtcgg ggtcgagcgc gacaagtcct
    33661 tcggtgattc gggccaggtc ctcgcgcggt gcgtttggag cggcgccgtc cagcacgccc
    33721 gcgacctcca caccggcggc gcggagagag tccagaatcg tacgttcgtg accggcggcg
    33781 ccggcaaagg cgtcgcgcac gagaacggca cggcgcccaa gcgacgcagc cagacgcccg
    33841 gtttctggaa gcacgccgaa tccgtggaca taggcgtcgc ccttgaactc attgatcaac
    33901 tcgtacgctt cgcgaaataa gggcggagtc attttatgga agctccttga ttcgaagatt
    33961 gcgaaactca acccggctgc cgtggccgat cagtatgata tgacccgacg ggcgggcgag
    34021 gccgggatgc ttggcaagca ccgcaggctc gcgcaccatg tcaagatcgg cgtcgagaat
    34081 gacgacgccg ttgagcgtga ctttgatgcg tcggccgttc gcctcgatct cctcctcttg
    34141 ccacgcgccc ggtttcttta gaaaaccggt gcgtgcggga atgacgtcat agacggaacc
    34201 atggtactgc tccggacgga gcttcattgg tccgtacttt ggcccactct ggtcaaggat
    34261 ctggatctcc attccttggg tggcggtatg gccttcacgt ggagcgcgga tgccgatgcc
    34321 gttattggaa tcgggctcga gacggaactc gaagcgaaaa acgaagttgg cgtattcctt
    34381 ttcggtctcg agtttctcac cgccgtccgc cgggcagacc aggacaccgt cctcgacaac
    34441 gaaaccagga ccctgcttgt acataacgaa ccagccgtcg aggtctttgc cattaaacag
    34501 cggcacgaat ccctctacgg caaaggcggc cgcggcgagc agcagaagaa gaatgaacct
    34561 cacagcggat gattgtgaca cagatcgggc gtcgcttcat taagtgataa tggcggcgtg
    34621 cgactgttga tctgccttct tctgatgatg gctcccgcga tgcgagccgg cgtggactgg
    34681 gtccgaaccg ctagggtttt cctgatcgat gggtaccagc cgcccttcgc ccccgaactg
    34741 gagtttgacg ctgaggcgct ggcgaaaacg atgacggaga tgaacgccaa cgttgtgcgc
    34801 atgtcgacga tggggaagta cgcgcacatc cagggcgtac gcttcacgcc tcaccgagcg
    34861 ctcggcaagc gagacctgct ccgggagatg atcgcggcag cgaaaccgcg gggcatccgg
    34921 gtgatcccgt acatctcgac gggccacaag cttgcgtggt cgatggtcac gaaggaatac
    34981 ccggagtacg ggcaccggcc tcgcccaggt ggccagccgg accggagcca catgttcgtc
    35041 ggcgaggacc atgggaccgt ctgctggaat acgccgtacc gcaaggcgtt tatggacatg
    35101 gtggaacaca tcgtccgcga ttacgagatc gacggtgtct acttcgacac gtggcgcccc
    35161 ttttatttct ggcaggggat gcggctctgt tactgtgacg gctgccggaa cgggttccgg
    35221 gcggcatccg gaaaagaaat cccgtaccac gaaaaacgga gcggctacac gaaggcggaa
    35281 ctcgagacgg tcgaccgcta ccacgcctgg tatcgagagc ggttcatcgg aatcttcgga
    35341 gaagtgtgcc ggctggtgaa atcgtacaaa gacataccgc tgatttacaa catcaaccac
    35401 gccaggcaga ttgcggaaga ggacccgcgg atcatcggcg cgatggacgt attcctctat
    35461 gagcgcggga actcgctgct cgagcgcgcc gaaggggtga gcctcgcccg ggcggcgggg
    35521 atcggcgtgt ggccgtacat cggggtctac aacaactggc cgcgagtcat ctacaacaac
    35581 gtcgactacc agcaggagat ctacaccaca gcgatgttcg gcggcgcacc catcatcgct
    35641 cagccctggg gctatgtcga tcacgcggag aaccggaagt tcgtgagcga tccgtttttc
    35701 gcgttgaagg cgcacgagaa ggagaccgag ggtttcgaga acgagccata cgtggcggtg
    35761 gtgtacgggc acaaggaccc gcccggatac gcgcgatcgg actggttctg gaaaacggat
    35821 gcacggacca acacgctcgg ggcgtttgct gcgtgcctcc acggccatgt gcaggtgtca
    35881 tctgtgccgg agcaaatcat ggatgaaccg gagaagctgg ggcgctacaa ggtggtgtac
    35941 ctcgccggca cgccgcacgt cagtgctcgc agcgcagcca acctgatgcg cttcgtggag
    36001 aatggcggcg ggctggtggt gagttacgga acatccctct acggggcgga gggcgtgcgg
    36061 cagacggggt tcgggatcga agaactgatc cgcgtgaagc cagcggcacg cgaagggaag
    36121 ctcgcggaga tcctggccag ctatcagtcg atgctgggcg ggccgaacga cctttacctc
    36181 attgcgcgca ccggcgcaat ggcggggaaa ctcatcccgc ttacgtattt cgagccggtc
    36241 gaagtgctcg atggcggcga tgtggcaatg gatatcgtca cgggggacgg acggcgtagg
    36301 gtcttgcccg gcgtggtgtt gtcgcgccac gggaaaggca aagttgtgta cggcgcgtct
    36361 tctctcgaag gactctatgt gcaggagagt cttcccgtcc tgcgcgacta cgtgaacacc
    36421 ctgatccgga tggcggctcc cgaggcgccg cccttcgaga cggacgcgcc gccgaccctc
    36481 gtcttcaacc tcacacggaa aaacagccgc agggcactgc atatcacgaa ctggagcgtg
    36541 agctccgaga ggggcggcgg atcgctggcg ccggccgaaa atgtaagggt tcggctgcgt
    36601 gtgcccgaag ggaagaaagt ggcagacgcg gaggtgtttg gggagagcgg ccacgctggc
    36661 gccgtgaaag gcacctcgcg cgtggtcgag ttgaactttc cgaggctgga ggcgtatcgg
    36721 ggtgccgtag tcgagtttcg gtagtgtata gtctgaaact atgcctcatc tcccagcgtg
    36781 ttccaggcgg cgttttctgt tgagtttagg cggcgcggga ttactgtccg gcctgcgggc
    36841 cggcgatcgt gtccgcccgg ccgggccggg tgcgaagtgc gttccggtga tcaaggctgc
    36901 gtttgtgcgc cgcaaggaag actacgggat gctctggccc ggagctatct acgacggcgc
    36961 tacggcgttg gcgaactacc ggcggcagat cgtggcagca tcggaggagc tcggtatccg
    37021 gattgacctg cggccggagc cggtttattc cctcgaagcg gcggatgcct gggtcgcgga
    37081 agcccgagcg cagaaggctg acggcttgtt cgtcgcgttg ctcgaccggc agcagcacgc
    37141 ctggccgagc gcggagaagg cgattacgag tgggattccc tcggtgatct tctccccgat
    37201 cggggcagca ttcacgacaa acacggggcc tttggcgacg aagaacggcg gattcatctg
    37261 ctccaccgac gatttccggc aggcggccta tggcctcaag atgctgaagg ctggcgcgaa
    37321 gctgcgcgag atgcgattta tcgtgctggc tgggaatgag cgaagggacg agaaggtcgc
    37381 agatttcggc acgaaattgc gatacctccc ggcacggact ttcctcgagg agtacaacag
    37441 cacgccagtg accgatgagg tgaggaagat cgccgcggcg tacatccaga atgccacggc
    37501 ggtgaagggg cccaccgagg aggatgtctg gaatggtgta aaaagctacg ttgtggcgcg
    37561 aaacatcctg atgcgcgagg aaggcgacgg catcacgatg gactgcctcg gggcgttagg
    37621 caagacgaaa gtcagcctgc cgtgcatctc gtggtcgtcg atgctcgatg acgggattcc
    37681 ggccgcctgc gaggccgata tcggagcggc agtcacacac gcactcgtgc agtatctctt
    37741 cgacaggcct ggctttcagc aggatcccgt gcccgaaaca gcgaaggact gtctcatcgg
    37801 cgcgcactgc acctgcccga cccgcctgcg aggctggtcg cagccacccg agccctactt
    37861 cctctcgtat caccacggca agcgtgatgc agtgccggtt ccgaggtgga acattgggga
    37921 gagaatcaca gtggccgacg tactcctccg cgaccgggag aacccgaaac cacaaatggt
    37981 catctccgtc ggcaaagtgg tcgaaaacgt ctctgttccc ccggcgggcg gttgcgtggt
    38041 ttcggtgatg gtggaactcg acggttcgcc ggagcttttg gactaccccg gctttcacca
    38101 gctcttcttc tacggagact tcaggcgcga gttgcaccaa tactgccggc tgttcggcat
    38161 tcagcctatc ttggcgtgaa agcgggcttg acggctgctg cgcctacgca gctccaaacc
    38221 gtcgcacaaa ctcggcgttc tccccaggct ggtcgctgta acggtcccac atcccgacga
    38281 tcacggcatc ggtcttcttg attccgttga aggcattgcg gaaggcctgc tcgatccact
    38341 gcgggtgctc cgacaggcgg ccggcggcga gaatcttgaa agccaggcag ggcttcttcg
    38401 tctgctgaac catccggtac atccgcggcg gatcgttcgg cagatacact tcgcccacag
    38461 ggattggcgc ctgcccgagc agtttctcga gctcctcttt tgtgcggtgc cgttcgtaaa
    38521 cgcaggtcat gtagtagtcg aagtcccagc ccttcgattc cactgtgtcc accaccgcgg
    38581 gcatgtgggt ggagattccg accagcatgc cggagtcgcg gaccatcttg aggtagtcct
    38641 gaatctggtc gatcttgccc tgcttgaaca gtcggtccgt gacctcgccg tgatgcgcaa
    38701 tgccaatgca gccgccgcgc gcgagcgttt tcaccacttc gggctcgctg tgctcgaggg
    38761 cgagaaagtg catcttgccg ccgctgtcca cgtaatggcg gtagttctca aggttccctt
    38821 tgcctgcctg ccagcaattg atccctactt cctcgcaacg gcgcagcgtc ttcagaatct
    38881 gctccggcgt atagtacttc ttcatctcgt aattgacgaa gaccgacaag tgcgatccgc
    38941 cgttgaacac gttggcgccg cagatgaggc gcgaaatcga gtagcacccg agctggacct
    39001 gtggcagctt ctgtctctcc gccggctggg cggcttgcgg ggccgccgcg ctgatcgaga
    39061 ccgcacccgc ggccttcagg aagtcacgtc gcgagttcat gcggttcacc cccaaaacag
    39121 atatgagcac cgggaagtcc gggtttcaac agtatacatc gcgatccggg atggtcaggc
    39181 gagcttctcg attgccgctg cggccgcgcc gaggacgccg ccgtaatagc cgagcggcga
    39241 tgggcacact tcgatgcggc gatgatccca agctgttacc tctcgggcga aggcagcctt
    39301 gaggtcgtcg aacagcatcg gattgttctc ggcgagccct cccccaatca cgagcagcga
    39361 cgggtcgagc agatggatga tgcctgtaag tccggctgcg agatagccgg caagcgtgcg
    39421 gatggcgcca cgcgctggac cgtcaccccg ccctgcctcc cggatcacta cctcggcgct
    39481 cgcaaatcca cctctcgcgt aacgcaggag cgcggcggca ttcgtgtaca cctccagaca
    39541 cccgcggcgc ccacaggtgc acggcaggcc gccatactcg atggtgatgt ggccgattcc
    39601 gttggccaga aagtgcgggc cccgattcag gcggccagcc acgtagcagc cgccgccgac
    39661 gccggttccc agcgtcacac acacgaaatc gtccgccccc cgcgccgcgc cgaaatactt
    39721 ctcggcgacg gccagcgcgt tggcgtcgtt ctcgacgcca accggcagcc cggcaacagg
    39781 accgagttcg gcggcgatcg gggcgcccgt ccacccgggt aggttgtccg tggcgtaggc
    39841 caccgcgcca gtaccggggt ccacccagcc ggcggtggcc acgccgagcg ccgagggctc
    39901 tacaccggcc tgcgccgcta gttcccggca catcccggcg acgcgcttca ggtgagcgag
    39961 tagcacttcc cggcctccgt ccgacggggt cggcacggac gctctcgcga ccagtctgcc
    40021 gtcgcgggcg actacgccgt atttggtgtt tgtcgcgccg aggtcgatac caaggaagaa
    40081 acggtgtttg ccgcttcgga cctggcactc gatcgcctga acaatgcgcc gggtgatcgc
    40141 ttcgggccgc gtaatcgcgg tgccgatgac gagagagaag gcgccgcccg cgaacgctgc
    40201 ggcggcttcg tccggcgtcg agacgtgtcc ttcgacaatc agcggcgtgt ccagttctcg
    40261 gacccactcc gccacataga atggctcgaa gcacgtcaca tccgcggtgt cgtcggtata
    40321 gccgcgcatg gtcgaagcga cgatctcggc gccagccgcg gctgcggtct tcgcttcctc
    40381 cagcgtggcg cagtcggcca tcacgggcac gcggagttcc gcacgaatcc tttggagccg
    40441 ctcgatcgca ccgtagtgcc tgccccgggc ggtgcagtcg agggcgatga tgtcggcgcc
    40501 cgcttcggcg agcgcatgtg cgccctcgaa cgtcggggtg atcaagattc ggccgtcctc
    40561 gatgagccgc ttgtcaatcc caagaatcgg aacggagacc gcggcacgga tggcacgcac
    40621 atcctcggcg ccgttggctc ggatgccggc ggcgccgccc agcacggcgg tgcgcgcgaa
    40681 acgagccatc gattccggat cgtggaaggg gtcgccgtca ggggcctggc aggagacaat
    40741 gagccgctga tgaagcgcga ggaggacgtt cgaaaacggt ttcatgcttt tccgaagctg
    40801 cttgacacag cgcaaaggac gatcgcggcg agcagggtga gaatgcccga tctcataaga
    40861 cgcagcgccc tggggtccgc gcctcgccat tcgccgagcc agagacccat cacgttggcg
    40921 acgaccaggc cgacggcgag gcttagaggc cagccgatcg aagggccgat gtcgccgagc
    40981 atcggcgtgg ccgcgccgta caggaagatg ctggcgcccc acagcagccc catgagcagg
    41041 ctgagtcccc aggcctttcc cggctgcggg ccagagaaaa gtgcagcgct tccgttccgc
    41101 ctgagcagca tggagcagta ggcgatgttc gggatagctc caccgccaag catcagtagc
    41161 cagatgaggt tggtgctgtc gaagctcgaa tacccgaggc gctcaccggc cgcggcgatc
    41221 gggagcgcca ggctgtaccc gatgttgaac actgcggaga gaatcccggc gccgatgcac
    41281 agcgcatacg ccgagaatag gcttccaccg ctgcgcgaac tcaccgacgc ggatgactcg
    41341 cggagccgtc cggcctgtcc gcagaaccag acaccagcga ggaacgtcgc tacgccgatg
    41401 aaaagaacga tctggctcag gtcgaatcgg agattgccgc tgagtaagag gggtacggcg
    41461 gagccgagcg cggagctgag tccgatgacg aggctgttgg ccagagagac accgagatgg
    41521 tgtacgctca gcccgaaaaa aatagccccg aatccccagg cgagcccgaa cccggcggcg
    41581 caggcgattg cgttgggggg agaggcggta aggacaagga ctgcgtccac acccgacgcg
    41641 accatggcag cgggcatagc cacgcaagcg atcaagataa agacgagcca gatgttctcc
    41701 cacttccatc gcgggatgag cttcatcggc gtggtgaaca ggccgttgca ggcgccggag
    41761 gcgagtgcaa gaaaaacgcc aagagcgaca gatgaacgca ttagcggacc aacagtggat
    41821 tctacgacac tgcgaggtgg tgacggcagg tgacgaaacg gcccgttacc gggagcggcg
    41881 ccgggcgccg ccacttcgcg tcgtgcgcct cgaacgcccg ctcgtcgtag gcggtccggt
    41941 caccgacgcg tcggagatgc tgcgtcgtag ttcggggtcc gagctatcct gggatgcttc
    42001 gaggtacgcc atagtcagat actctttgcg gctatcgctc cagcgcgcaa attcgtcggt
    42061 gtccttgcgg tcgaacttct gaggagcgcc gctgccgtcg agagaaagga gtttcccctt
    42121 ttcaacctcc acgctctggc tgcccaagcc gacctgtgcc ttgccatcgt acacgcggat
    42181 gcacgggggg gccgcggcat cgaagcggta cagcccgctc ttccgcagga cggccggagt
    42241 accgttcgcg acaacgctta ccgaactgcc gcggagctgg ccggcgtcga cgatgatgga
    42301 gccagccagg aactcgacct ccgcgcgtgt gatcgatgcg gagaggaggc ggacactgct
    42361 ctcttcggct agacgaagga aaacacccgg ctggagtaac acctcagcgc ggcccgcacc
    42421 ggtgcggaac aactgccctt ccggaataag cggataatcg cccggctggc cgattacagc
    42481 ctggtccccg agaaataccg ccccttcgac gtaatggacg agtccggagc gtgccgagat
    42541 aactgtttgc gcggcggcag gcaaagtgga caggccggcc agcagcgtcg aaacgacgac
    42601 cgacttccca gcccagtgca tgactggaat ttgccacaag ggggagagaa atgcaagggg
    42661 cgtggaggag aaatctcaag gattaagact tgtgaaccgt ggatgttgat tgaggcccgg
    42721 gcgaacagcc cgggcttttg gtagggatta cttcttcttc ttcgccgggg ccttcttctt
    42781 atctggcgcc ttggcggttc tcgttccaca agccatataa agtcacctct caggaaatga
    42841 tgaacgagaa tatgcgattc cgtcaagagt tgagggtttg attctccgtt agagtcaact
    42901 tccacgctat tctggagacg tggtgtttca gggagtttgg aaggcggcgt tggcggcggt
    42961 tctgctgctc agcgcttgcg gatgccagta cgttgggcga acgtcaaggg cgacgcggga
    43021 atcgatgggg tacttccgga acgatgcgtg gacgatcatc ggtccgggag gcggaggcgc
    43081 gcagttctat ccggccgtga gcccgcacga cgcgaatcgc gtggtcgtgg gttgcgacat
    43141 gaccggctcg tacctgtcga ccgacggcgg gcgtggctgg cgaatgttct ccctgcgcga
    43201 accggcgcgc ttcttcgtgt gggatcccgc gtctccgaac atcctgtatg cgcagaccag
    43261 tgcgttgtgg cgcagcgccg acacaggcga gacgtgggaa cttgtctacc cggatccgtc
    43321 catggtcacc ggtgtcgcga tgcccggaga ccatggcgac ggctggatca tgacatcggc
    43381 cggctccttc gataccgtgg ccggcctcgc catcgacccc gccgattcga acatcctcta
    43441 tgccgtggtg acttcgtcgc gtggatcgta cctgcgcgtc tcaagagatt ggggacgatc
    43501 gtggacgtgg gcggcggacc tcccgcaagg ctcgaaagcc atttacatcg atccggactc
    43561 gccgcgggct gaccgcacca tctacgtcgt gggcgacacc tacgtgctct cccgtgtggc
    43621 gggaacatgg aggcagggtg cgccgactcc gggctacgcc gtcatgacaa aagtcgcggc
    43681 gggttttgcg gacggagccc tggtgattta cgccatctca gcgcgcgggc tgctgatttc
    43741 gagggacggc gggcagaact gggtggactc ctacgtctcc atgcaactcg tggccgtggc
    43801 gacgccgctg tggagcggcc ggacggctta tgtctcgtat ggagaactgc cagaaggcgg
    43861 ccgctcgtat ttcggcgttg cgaagactga aaacgcgggc atctcgtggc aatacccctg
    43921 gaaggaagca ggaatgaagt cgcccgatgt ggatgacggg tggatgagcg cgcgcttcgg
    43981 tccggattgg cccggcccgc cggtcagtct gggaacggcc ccggacaacc cgcaaatctg
    44041 ctacggctcc gactacggcc gtacgctccg cacgaccgat ggcggcagca catggcgctc
    44101 tgcatactcg taccagctcg cggacggctc gttctccaca cgcgggctcg atgtgaccac
    44161 ctgctacgga gtccatttcg accccttcga cgctcagcgg atgttcatct cgtacaccga
    44221 catcggactt tttgttagtg ataacggcgg gaagggctgg cgtagctcga cgggcgtcgg
    44281 cgtcccgtgg gagtgggtga acacgactta ctgggtggag ttcgaccccg aagttccagg
    44341 ccggatgtgg gcagtcatga gcggtacgca cgacctgccg cggcctaaga tgtggcggac
    44401 caagtcgccc tcttcctacc gcggcggcgt cgcgaaaagc gaggacggcg gacggtcgtg
    44461 gcgcacgctc atgaacggga tgccgcagac cgcggcgacg cacatcctgc tcgaccaccg
    44521 cagcccggcg ggcgcccggg tgctctacgt ggccgggttc gggaaaggcg tgttcaagtc
    44581 cacggacggc ggcgaaagct ggcagataaa gaacaacggg ctccccgcgt tcgagccgtt
    44641 cgcatggcgt ctggccatgg accgcgacgg catcctgtac ctggtggtcg cgcggcgcag
    44701 cgagaacggc agcatcggga acgaccagga tggcgctctt taccgttccc gcgacggcgc
    44761 cgagacatgg gaaaagatgc ggcttcccga tggtacgaat gggccgaacg gcctggcgat
    44821 cgatcctgat gatccccgcc gcttgtacct ggcggcgtgg gggcgccgtg ccggcagcca
    44881 ggctgccggt ggcgggatct tcctctcgac ggacgctggt gaaacgtggc gcgccgtgct
    44941 cgacaaagat cagcatatct atgacgtgac catcgatccg cgcgccccgg cgctcttata
    45001 cgcgagcggg ttcgaatcgt cggcgtggcg gtcgaccgac cgcggagaaa catggcgacg
    45061 catccgcggt tataacttca agtggggaca tcgtgtaatc cctgactcgg ctgacatcgg
    45121 gaagatttac atcaccacct ttggcggcag cgtctggcac ggacccgctc aaggtgacga
    45181 ttcggccgtc gaggacatcg tctcgccccc ggcgctgcga tactagccgc cattccttct
    45241 tgatccggtc gtgcgcccac cgggaagcct tactcgaaaa ttcactctcg ccgccacaat
    45301 ccagttggca tatacttgtg cggacccgag gtttcgaggt cggacgaaag ggggatcgta
    45361 tcgtgaccat gagtaggcga gctgccgccg agttgatcgg cacattctgg ttggtattcg
    45421 gaggctgtgg cagtgccgtt ttagctgctg catttccagg tcttggtatc ggttttctcg
    45481 gtgtggccct ggcctttggc ctgaccgtgc tcacgatggc gtacgccatc ggccacatct
    45541 cgggttgcca tctgaatccc gctgtgtcgg tgggactggc ggtcggaagg cgttttccgg
    45601 cctcggagct gctcccgtac atcaccgcgc aggtggccgg gggaatcctc ggtgcaggtg
    45661 tgctgtatac gatcgccaga ggaaaaatag gctttgatct cgcggcggga tttgccgcca
    45721 acggctatga tgcgcattct ccgggtgggt acacggcagg cgcatgcctg gttgccgaag
    45781 ttgtcctgac gttcatgttc ttgatgatca ttctcggtgc aacagaccgg cgtgcgcctc
    45841 agggttttgc tcccgtcgct atcggactcg gcctgactct tattcacctg atcggcattc
    45901 cggtcactaa tctttccgtc aatccggcgc gcagcacggg tccagcggta ttcgttggag
    45961 gttgggcgct tgaacagctt tggctgttct gggttgcccc cttggtcggt gcggcgctcg
    46021 cgggaattgt ctacccggca gtcgccgggg acagggaaag ccggtgagaa gtagttgctt
    46081 ttctcactgg aggacgattg gaaggacata gttggaggcg aggttcccat acctcgaagg
    46141 aggctgacat gatgcacttt ctatggatgt gtcttatcgg tctggtggcc ggagcgcttg
    46201 cgaagctgat tatgcccggc aaggaccccg gcggcatcat catcacgatg cttctaggta
    46261 ttgccggttc cctggtggcc ggattcctcg gacgtgctgt agggtggtat gaagccggac
    46321 agggtgcggg attgattatg tccgtcatcg gtgcgatcct gctgctagcc gtctatcgct
    46381 taatgaagcg aggatcggcc tgagcgggcg gcatccgcca tttcgcgatc tgttgacaac
    46441 tagtttctaa ggtgcggtcg cttgccgatc aagaaaaaga tctgtctgct gggtgctgag
    46501 gcggtaggga agacaagcct cgtgcgccgg tatgtcctgg ggatcttctc cgaccgttac
    46561 ctgaccacga tgggggtcaa ggttgacaag aagatcctgc atgtcgctga agaagaagtc
    46621 cacctcatcg tgtgggatct ggccggtgag gacgagctga ctcaactgcg gaccgattac
    46681 ttaaggggcg cttccggcta tctccttgtc gtggatggga cgcgcatcgc cacattgcac
    46741 aaggcactcg aactccagcg gaaagcggcc gacgcggtgg gttccgttcc gtttgttctc
    46801 gtgatcaaca agtcggacct cgcggacgaa tggttttcgc ttcaggacga actggaagag
    46861 tctggctgcc gcttctggcc gctttgtacg acaagtgccc ggctggggac cggcgtcgaa
    46921 gaggcattct tgatgcttac acggctcatg cgggcccatc aggcggaacc cggctgagct
    46981 gttgcggctc tcgaacggaa atgtcgaata tcgagatctt gggtttgttg tcggcgctgg
    47041 acaacgcagt gctgcgcaag gacgagaaag ggcgctggcg tctcgttgag acacctcccg
    47101 agtggttcat aaagctctat cctggcgctg aatctccgtc cggcctccat gctgaggaat
    47161 ggtcgccgtt tctggaaacc ttcctggaga atgccggtga cttcctgtgc gagagcccag
    47221 gcggacgcct caagtccggt ccctggacgg agagcactcc cgatggcgaa gagattgata
    47281 tggaggccac catcctccgt cacaaaggga agacactgct ggtcctgacg caccgtggcg
    47341 aggagtacgg ggaaaggcgg cagatactcc aaaaagcccg tgaaaaatca ctgaaatatc
    47401 agaagctgga gcgcgttcac gatgagctcg ccctggtgtt gaatcagctc gaggtggggg
    47461 cacttaccac agacgctgcc ggccggtgcg tcttcctcag tgagcccgcc cggcgtctct
    47521 tcgacatgga tgagaaggag gtactcggta gacggtggct ggacctgaag atcctcgata
    47581 gcgcaggccg gcgcctcctc gaactcatgg ctaggcttct gcctgccgag cggacgcggg
    47641 ttcccttgca ttgggagcga gacgatggac gggccttctg gatggaggtg gacctgcgcg
    47701 accaccccaa ggatgcccga aagaagatcg tatttctgta cgacgtttct gagttgcaca
    47761 gcttgcggcg cctcttgggc gatcagggcc agttcgaaaa cctggttggc aagaacgacg
    47821 cgatgcggcg cctctaccaa tcgattgaaa atctcaccgg tgtcgacact accgtactga
    47881 tcgaaggcga gactgggacc ggcaaggaac tcgtcgcccg cgcgatccac aatcgcagcg
    47941 agcgccgtgc acgacccttc gtggctatca actgcgccgg gttaaccgag tccctcgtct
    48001 ccagtcaact gttcggtcac aggcggggcg ccttcaccgg cgctcttcag gatagccccg
    48061 gactctttga ggcggcggag ggaggtgtac tctttctgga tgagattgga gacctcccgc
    48121 tggctgtaca gacgagtctt ttgcgagtgc tccaggagag ggagataacg cgcctcggcg
    48181 agacaaggac gaggaaggtc aatgtgaggg tgcttgccgc cactcaccgg aatctcaccg
    48241 aagagacaga gagagggaac tttcgcgccg acctgctcta ccggattcgg gtggcgcgaa
    48301 tccaaattcc ggccctccgg gatcacctcg aggatattcc gctgctcgtt tcgcgctttc
    48361 tggccgactt ccgcgcctcc acggggaaga tggtcttgga tgtttccgcg gaagccatgc
    48421 gcgctctcat gcggttcccc tggcccggca atgtgcgcga gctccggagt gcgattgagt
    48481 ttgcagtgat caactgccgg cggccggttc tcgctctcga tgatcttccg cccgagatct
    48541 ctgcccgtcc gaccggaacc atgcgggctc ccacgccgga ggctccgccg gcggacgaga
    48601 aggagcgcat tctctccgcc ctccgcaagg cgggggggaa gcgcagtgcg gccgcgaaat
    48661 tgctcggaat cagcagggct accttatacc gccggatggc tgatctgaag attcccgccg
    48721 gcgtctgacg gtcacacccc gcccggccgc tgtgtctcgg gttgagacgc ccctctatca
    48781 gtgagacact cgtgagacac cggtgtgttt catccggtgc tcaccccatt tcagccgaaa
    48841 tcgacctcgt gacacaagtc tctcagcttc tttctgttac agaccctggc cgcgtttggc
    48901 acaggtattg cgtattcccc tacgggtagt gggggactgc agcttgcgct cgcgaccacg
    48961 actgttacgg cacgggcgga cttagctgat catacgcgcc gtcgttccag tacatactgg
    49021 ccacggctgg cgtgattcat aactggggag ggcagatcct tgactgctgc tccccttttc
    49081 ttttgatttc ggtgcgtggt gcaatgatga cgggggacga ttcaagttga ctgaaatacg
    49141 tttgagatct gcagtccttt ttgtgttgct cctttgcgcc gctcatgcgg gccgggctga
    49201 tcagatcacg ctgaagaacg gcgaccgggt gacaggcgcg attatcaaaa aagacgggaa
    49261 gacgcttact ctcaagacgg atgctttcgg tgtcataacg gcgccttggg accaggtggc
    49321 cgccatccaa tccgatgcgc cgctcaatgt ggttctgccg gacggcaaga cagttcaggc
    49381 gacggtgcag acttccgcgg gcagggtcga actgaaagag acgaggcaga cgctcgcgac
    49441 ggaggaagtt gtcgcgatcc gtaacgcgga tgaacagaag gcctacgagc ggctgctgaa
    49501 tccaggctgg gcggatcttt gggccggcac ggcaacgatc ggtttcgcgg gtacgcaggg
    49561 aaacgcgcaa acccgtacgt ttaccacagc cgtgaatgcg gcccgggtca cgaatactga
    49621 caaaacgagc ctgtatttca atgccgtcaa ggcttcggct ctgctcgaag gggttagcgc
    49681 gggtacggcc caggcggtcc gaggtggttg ggcgcacaat cacacgatct cgtcccgctt
    49741 cttcgtgaat acgttcaacg attacgagta cgaccgtttc caggatctcg accttcggtt
    49801 cgtgctcggc ggcggcttgg gctacaacgc ctggaaagga gagcgtggcc ggttcgatct
    49861 cgtcggcggc ctcgcgtaca accgtgagaa gttctcgccg ccggctccgg cgtccgaatt
    49921 cactcgcaat tcggcggaag cttattgggg ggaggactac aactacagat tgaccgaagc
    49981 gacttcactg gtccagagtt tccgaatgtt caacaatctg tcggacacag gaacgtaccg
    50041 catgaacttc gatctcggag ccaataccag acttttcaaa tggcttacct ggaacctggg
    50101 cttcagcaac cgctatctca gcgaccccgt cccggggcgt aagacaaacg acatccttta
    50161 cacaaccggc atcggtgtga cgttcgcccg gtgagggctt ggattttcga actaacagcg
    50221 gcattcttag gaggaaaaag aagtcatgaa ccatcaaaga ctgatgcact tgttttgcgg
    50281 tctagctgtg accattctgc tcgcaacgtt ttcgccaatg tgggcccagc aggcggggac
    50341 tgggttcttg aaggttaagg caaacaccgg acgcgccggt gtgttcgtgg acggcaaata
    50401 ccttgggccc gcggcgaatt tcgccatggc gcggaagtac gtcgtggcgg cgggcgaaca
    50461 cgacctcgtc ctgcgcgaac cccgttacca ggagtgcaaa acaaccgtga agattgacgc
    50521 gggcaagacg accacggtcg cccagtcgct ccagccccgc gcgctggcaa ctccgccgtt
    50581 cgggaggctt cggctgactg gtttcgaaaa gtacgcagcc gtgtacgtga atgagggcta
    50641 catcgggcac gccgacgaat tcagcgcgcc tggtcagggc ctcttgctga atcccggcga
    50701 ctacaacctg aaagtcacct cgcaggcagg tagcactctt ctcgagcaga agatcaccat
    50761 tcgtcaaaac gaggtcgcga gggtacaggc caaataagaa aacctcgaac agcaagaggc
    50821 cgatctggag ccaacttcgc ccggccgtcc ggaatcgatt cctttgacct gacaattctg
    50881 aatgcccaag ggcagttcca cctcgaaacc actcatgaaa gcaggcaggc agaaggactc
    50941 ccttccgaac gacgggactg gtgaattgcg cccggaccgg ccggcgaatg accccgcctc
    51001 tgacgatctg cttccggacc tcgatgtttc cggcaacgag attcttgagg agacggatat
    51061 tgcgccaagc ccaagcgccg aggacgtcga agaactgggc gagttcgaac tcgatgccga
    51121 gtcttgggca agtaccgatg accccatccg cacgtacctc cacgagatgg ggagcttctc
    51181 cctgcttacc cgggagggcg agatcgatct tgccaagcgc atcgaacgcg gccgtgcacg
    51241 ggtgaggaag gccctgtcgc gggttcctct ggttattgac gagatgctca agctggtcga
    51301 ggcggtacag caagggcggg tcaccgtgcg ggatgtactg gtcaccccgg agacggcgga
    51361 catcgatgat tccggcgcga agcagagaga ggaactcctc cagtggctct ccgagattgc
    51421 caagtaccag gaaagggccg agcgagtccg ccagaagctt caggatggct ggcgcggcat
    51481 gagaccgaaa cagctcagac ggctgcggta caacacggct cgttctttgg tgaggctttc
    51541 gcggatctac cgtcaggttc cgctcagacc ggagttcgag cgtaagctga tcgagctgat
    51601 caggggggcg gtcgcgcagt acagaccggt ggagcgagag attgcaaaga tacagcggag
    51661 gttggagcaa agtgtgctgg ccggctcagc cgggcagaag gaggaactcc gcacgtcgtt
    51721 gcagcaactg atccagcggt tgcggaagct ggagaaggat tggggatggg gcgccctgga
    51781 ccttcggcgc acgctgcgga tcatcgagcg gggggagcag gaagccgaga cggcaaagaa
    51841 acagctcgtg gaggcaaacc tgcggctggt ggtgtcgatc gccaagcgcc acactgaccg
    51901 cggcctgcag tttttggatc tgatccagga aggcaacatc ggtttgatga gggctgccga
    51961 cggattcgac taccggctcg ggtacaggtt ttccacctat gcgacctggt ggatccgcca
    52021 ggggatcacg cgtgctcttg ccgatcaggc acggaccatt cgacttcctg tccacatgat
    52081 cgaagccatc aggaggctcg cgggcgcaca gcacgaactc aggcaggaac tgcgccggga
    52141 tccgacgcct ggggagctgg ctcggaaatt ggagatctca gtgagcaaag tgcggaggat
    52201 tctccgggcc gcacaggaac cgatctcgct ggagaccccc atcggggagg agatggactc
    52261 gtctctcggc gatcttctcg tggaccacga agccgtctcg ccctcccagt cgatcatcag
    52321 cctgaacctt cgggagcaaa cggccgaact tctcaggacg ctcaaaccca gggaagcgga
    52381 gatccttagg atgcgcttcg gattgctgga cgaccgcgtc tacacccttg aggagattgg
    52441 cctgcgcttt gcagtgacac gagagagaat ccggcagatc gagatgaagg cgttgcgcaa
    52501 gcttagaggc cacggccgca gccatcaact gaggaccttt ctcggaggca cctcgagagg
    52561 ctgattcgtg cggatcgcgg cttgaaagta acataactta gtagacttat acaaaaggaa
    52621 aaacagcaac cgtgtcattg aagattgaag aagtagaagg catcggtcca gcatacgtca
    52681 aaagcccatt gctgccggtg tcaagaccga cgcggacatg cttgacaagg caggctcggc
    52741 caagggccgc gcggaactca gcgagaagac tggcatcacc ggaacgctga ttttgaagtg
    52801 ggcgaacgat gtcgacctga tgagaatctc cggagtcggg ccgcagtatg ccgaacttct
    52861 ggaggcagcg ggcgtcgact cggtgaagga gctcgcgcag cgcaacgccg cgaatcttgc
    52921 cacgaaactg gctggagtga acgccctgaa gcacctggcg ggtacgtcgc ccgcggaatc
    52981 tcaggtcgca gactggatca gtcaggcaaa gaagctggct cccaagatct cttactgacg
    53041 ttacgcagca ccgaaagtgg gggaggcggg gatccctccc cagcgaacga aagcgggtca
    53101 agggccgctt gacgtgaccc gtcgtacccg cgcgccgtct ctgcccggca gcgctgctcg
    53161 ctctcgtcgg gatgctcgcc gagttccttg atgagtgccg cgtgcatcgt ctcgatctgg
    53221 ggaccgtgcc cggtcttctt ggccaggttg cggaactcgc cgggatcgta ttcaggttct
    53281 agatctctct gtcgcaccgt atgaatcact gttcggaccg cttcataaac gcagcgactt
    53341 gctattatcg ttgtcgatgt ccgtgccgtc ggcgttcgcg ctgggccttc tactcgtgct
    53401 gcgatcggtc gcacaggata gcaccgcgag tctcactggg aaggttgagg atattaccgg
    53461 cgctggcgtc ccccgcactc atgttgaatt acggtcagag acgtcgcctg acagagcatt
    53521 ccacacccaa gctgacgata ccgggagcta tcgcctttcc gggctacctg cggacaaata
    53581 cactttgaac ttgtcgagcc cgggtttcca agagctaacc gtaaagtcaa tccatatctc
    53641 agatggtgag cagaaactga tgccggttct agaactcctt gtgggttccc tggcggattg
    53701 cggtggtcac gctgcactcg actattttcg gttcctgcct ttgagaagtc acgtcgggaa
    53761 tcttcgtgga accgtaaggt tggaccaagg acccatggtg agaaacagtc cgactatcgc
    53821 tggtgctgac gtgatgttga tatgcgggag ggacgccgtt tgcggtaaga cgaagaccga
    53881 ttcaaacggg gaattcgttt tcagggattt ctctgccggc agttttgtgg ttcgagttgc
    53941 ccgtgcaggg ttctacccgt tggacacacc cggctattac attagacaac gaagggagtc
    54001 agtctaccat ccgatatata tcgagcgctg cccgctcggg gactgcgatc cacgtcaccg
    54061 tccaaggaaa ccgccggctc gctgtgagtg aatcctgcca caaaccgacc gcctccgatt
    54121 ctgcctccag ctttcgaacc tccttgctcg tgacccgttg ggagtccggg gcaaggtcaa
    54181 gagttgccaa gccgttcgcc tcgggaacac tctccggagc acgttctctg ccggcagctt
    54241 ccgaatcctt tgcgtcccta gcagactctg ggggaaattc ccttctccaa ttcgcgtgaa
    54301 ttcgcgtcca ttagtggccc ttcctcccca tccccacgac gtcacccgtc gtacccccgc
    54361 gctgtctctg cccggcagcg ctgctcgctc tcgtcggggt gctcgccgag ttccttgacg
    54421 agtgccgcat gcatcgtctg tatgcgcgat ccgtgcgccg attccttggc caggttgcgg
    54481 aactcgcccg gatcgtcctt caggttgtac agctcccgct cggcggggtg cgaggcgtcc
    54541 ggcgcggagt ggtacacgta cttccagtcg cccgtgcgga gcatggcgaa gcccgaggcg
    54601 atgttgtgcc cgtaatactg gctgaccgcc cggtctttcc acggcgactt cgtgtcgtcg
    54661 agccaccgcg tcatcgacgt gccgtcccag tcgtccggcg tccgcgtgcc accaatctcc
    54721 gccagcgtcc ggacgagatc taccgtggaa catgcgcccg cgcggcgttg tccgcctttc
    54781 catctctccg gccagctcac gatcagcggg actcgcgccg cctgttcgta cacgcagttc
    54841 ttccaccaca gcccgtgctc gcccatgttc tcgccatggt ccgctgtgta gaccaccacc
    54901 gtgtcgcgcg cgagccggct ccggtcgagc gcaccaagca cttttccgat ttcatcgtcc
    54961 acccactccg tcagcccgtg gtagagttca cggccacgcc gggtggcagc cgcgtccatg
    55021 ttcggaaggc caaagcccct gcgcaggtgt ttgtagttca atggcatccc atcgagaaaa
    55081 ccgtcaggga tctccggcat cggcactttc ttctcaaacc gccgccagta gcgctcgggg
    55141 acgattagag ggaaatgcgg cgccaggtaa ccgagaaaca gaaagaacgg tttcttcgcc
    55201 cgcttgcggc gtgaaattaa atcgagcgcc ccggcagtca cacggcgatc gtgggacagg
    55261 atgctcgagt cgtccccggg atgaaagtcc ttggtccggt cccggcacga tttcacgttg
    55321 acactttcgt cgtccgcggc gcgccggccg cctcgccctg tcatgttgct actgttcatg
    55381 ttgccgccga tttcgacgaa accgtagcgg tgcgccgcat cgtagtgttg tttaccgcag
    55441 aggaaggact catagcccac accgttcaga aggtgaggca gtgaggggtg atcgtctgac
    55501 ggcagccggc aactgttgtt ccacgcgctg atccggtgga tatacttgcc tgccgtgaac
    55561 gacaggcgtg aaggaacgca tagcggcgag ttgcagtagg cgtgctcaaa ggtgacgccg
    55621 cgctccgcca gccggtcaag atgcggcgtc cgcgcaagcg agttgccgtg gaatcccgcc
    55681 acgcgcggat tgtgctcgtc cgtcatgatg acgaggatgt ttggctgccg ttttcccgga
    55741 cggaccgccg cagccagcgt actcttgaga aagtcccggc gattcacagg cgtactcctg
    55801 ttcctcaacg ctccaaacgc ttcgtcagat tgtccagaac gtttcggtcg atctcgtaac
    55861 cgagccccgg cttcgtcggc gcgtaaacgt aaccgtcctt cgccacgcgg atgcggtcct
    55921 tcatccacgg gcggtcatag acgccctgcg gaaccgtcat ctcgaagaat acattgttcg
    55981 gcatcgccag ctcgcagtgg aagtgaacag cgtgatcgag agtgtctccc cagttgtgcg
    56041 gtgcgcactc catgccaaag cactcggcca tcgccgaaat cttcattccg cccgtaatac
    56101 cgccgacgtt atcgacgatg aagcgcagca catccacagc cccccgccgc acgtactccg
    56161 agtagtcgta aatcgagaaa atgaactcac cggcgtgaat gggcacatcc agcgcctggc
    56221 agagctgcac atagccctcg acatcggtcg tcggaatcgg atcttcgtac gcgacgaaac
    56281 ccaattcgtc cagcagcctg ccgacgcgca tcgcttcgtc gcgattgtag acgcccaccg
    56341 ggtccatcag cagcagaaag tcctcgccca ctgccttccg gaccgccttt gacacctcca
    56401 tgtcgagcct gtaattgctt cctccgtgcg gcggtacctc cggcggatgg atcttgtacg
    56461 cgcggaaccc ctccgccttg catttcagcg ccgattccac aaactcgtcc acagtcctca
    56521 ggtgctggga gctggcgtat gcgagcacct tgtcgcggta cgcgccgaga atctggtaca
    56581 ccggaagccc gactgccttg ccgaggacgt cccacaggca gttgtcgatc gctgaggcgt
    56641 acgagctttg cccccgccgc cgcttggccg gaacccattg cgaggtgagc gccggtcgat
    56701 ccagggcgct cttcccgagg aggagaccct tagcgtaacc gagccaccct tcgttgttcc
    56761 agtccggatg ccagtatcgc gtgccgagcg tatagttgcc ttcgatgccg gaggcggtga
    56821 cgacaccagc gagcagtcca tggtctgtgt ggtagaccct gacctccttg atcgccagat
    56881 ctggcaactc cgacgccttc ataccggctg cttgaagtgc gcccgtcgcg ggcgccgctg
    56941 ccgaagcaga aagaaagtga cggcgagaca acgtcggaat cttcatggca ttctccgttt
    57001 cacgctacag ccggaccatc gcctccgtcc attcgcgcag gcggtaagac gagaattcta
    57061 catcggccgt gatgaccgcc agaccgttcg cttccggcgt gggagttatc gtcatcgccg
    57121 cggatgcgga cccgccggcc tgagtgcgta tccggtacgc gggctctgag gccttccagc
    57181 cggcgggcag gttcagtcga acagtgaatt cctgtaccgt cggcgcgtgg ttccagacaa
    57241 acgcctgaag ctggaacggg cggccgggcg ctgccttcac ttcgtagggc gcgagacggc
    57301 accactgctc gtccaggccg aaatcgatcc caggccacgg gaacaaaggc tgcatcgcag
    57361 ccgctcggcg ctgcaacgac ctttgcatgt agttcaactg ttcctctgtg aatcggaacg
    57421 ccggctctac gtgctggttg acgagccacg tgtcgcgcgg caggcggcgc acctgttcca
    57481 tgcaattcca gaaaccctcg ccaggcgaga ggaaatcgcg gttccataag cagtagtcgt
    57541 cgatgccgga gggagtgaac gaatccccga cgaaaaggat ggcctcgcca ccatctttct
    57601 tcgcgagcag gctgtcgtgc cagagcgtct gcccgggaaa gaaagagacg gaaagttcga
    57661 actcgtgcca ccgccacctg tggccgtgcg cgagcgcctt cccggagcga atcggaaagg
    57721 tcgtgagaca gggtgcccgg tacgccgacg ggttctccag gatgtcacgc aattcggagg
    57781 tgaagtacac cggacagccg aaccgctctg cacagacctg cgcacggtcg gtgtggtcgt
    57841 cgtgatagtg cgtgatgaag acgcctgcca gcgttcggaa acgaccgctg tcccgaagtt
    57901 cggccacctt cgcgacgatg tcgtccgaac cacagtccac gagaaacgcc gctccggact
    57961 ccgatagcag gaggcgcgag ttgctgatcg tcaccgccca acccgggagc gcgcgcgtct
    58021 ccgccatcgg catccactcc gggacctcgc cttcaagcag cttggccgct cgcagccgga
    58081 ggttatcgtc cttgaagtac cagcgcagtg cgtcagtggt gaagtgctca cgcagaggcg
    58141 tcttgagtcg cgtgatcaga ttgtcgatcg cctcggtggg gcgctcaatc acaggacccc
    58201 gcgcgggaac gagcacgtcg ggtttttgcc ccgctaccgc acggagactg cggatcagct
    58261 cgccggcacg ggcggcgtaa ccatgatacc cgcgcagttt tgcctcgggc accgcgtcct
    58321 ggaggctcca gaggtcgaac aattggccgt ccccgtaaat gagatcgccg gtgcaagcga
    58381 tgcgccggtc atcgacggta acgaggtacg acaccgcgcc gggtgaataa ccgggggtgt
    58441 cgagcacgtc gatgacgagg ccctgccact cgatccgctc gccgcctcgg actgcacgcg
    58501 ctaccgggag cggctcggtg agcacgcggc tgttcagcat cgcatagtcg tgataccgcg
    58561 ccttctcgaa ggactgccag aatttgccgg ggtgcgcgat cagttccgct tcagtggctg
    58621 gtgcgatcac cgttgcacct ttgcgttgca gttcccgcgc ggcccagacg acgtccctgc
    58681 gagaatgcgt gagcagcacc tgttccgcgg aggccggcga cggccgtgga tctccgtaca
    58741 ccgcgagtcg tcttccgccg cgagtgaata tcaggccgtt caccgcaccg ggcacgactt
    58801 cggcggaacg tgtaagttgc gcgctggccg gagacgccag caaacagaca aactcgcggc
    58861 gtgtcatcgc acgagcgatt atattcaatc gcggaagcgc agcgaaaaca ggtcggcatc
    58921 cttcagcacg aatcgcaggc gcacgggctt tcccgccagg ttcgacacgt cgccgcctcc
    58981 cgcccaagaa acgttgcgca caacctggtc gccgatgatc tcgcggcaat cggcgagaga
    59041 gaagcccggg accggcttgc cctccccgtc ctggatctcg atacgcaccg acccggcggc
    59101 cgaagtcgcg tagttgatct ccagcgtccg cccgcggaac cgtagcagct tcgtcaacat
    59161 ctcaccgccc gcatagcccg cattcacgga ggcgaacccg tccagccgta gtgcgtagcg
    59221 cccgacgtgc gccgtcggct gtccgtagtt gcggttgaca tagaacgaca tctccgccgg
    59281 tcccgtctgg acgacgttca gcgcggggta gttggtacgc gatacccagt tctcggcgcc
    59341 gagtcctgga cggaggaagg cttcaaggaa cgtccggtcg tagcgttgac cgccgcgcgt
    59401 cgaaaagagc acggcgtcgg aacaatcctg gaagtacttt ggatccacgt ggatagcgcg
    59461 ggcttcgtct tcggtcaaca cctgccgccc cgggaagaaa cgggcggcga tcgcaacgta
    59521 gatgtgcggc gcgcggaaat acgggctggt ctggttcgtg tacaggtgct cgggcggagc
    59581 gtcgccgaac gacatctcaa ccggtgtgtc ccagtggacg aaatcggtgc tggtggtccg
    59641 ggtcacccat cgaacatggc gcttgtccgg taacaccttg aaagtacgga agtaacacac
    59701 gtagctgcct tcgctttcag accagaaggc gagattctgt gagtcgtagc gggtctcgcg
    59761 cgctggcgtc agcaccggtt cctcgcggag cttacgccag cggatgccgt cggccgagac
    59821 aaaagcggcg agcccggatt ccgaggtgcc ggccagcgct ttgtactgtt cggagggcgg
    59881 aacccccgga cgggtgtcca aaagaggtgt aaaattgtgc gaaaatggcg ctttttgcgc
    59941 caaaatgacg ttgttttggc gcgtattttg cacctcgtag aggccgagat cgggccgcga
    60001 ccatcggatg ccgtctgggg actcggcgta gcaggtcgcc tcctcgatcc gcccgtcctg
    60061 cccagagttc gggacgcagc ggtagtaaag ccggtaccgg tttctgtcct tgaggactgt
    60121 gaaataggcg cagaaggccc cctcccacgg ccggtcaagc gcaagcacgg tccctttctc
    60181 aaccggcgtc tggaggcgca gggacgcgcc gtccaggcgg tcgatcaggt gacggtccac
    60241 aaagatctcg cggcgtgagc ctatgtcgat ctgcgcgaag gcggaagcgg tcagcgccag
    60301 ggtgaggagc gcgcgcatgg agacatcgta gcccggaatc gcaccacggc gtacggtata
    60361 ctcggggcgg attggagagt gtgtggaaat gaatcgcaga atgtttctgg caatggcagc
    60421 gttgccggcc gcggtccggg cggaggattg gccggcgaag agcggcgtct tccggcagga
    60481 caacctggtc gcgtggtgca ttgtgccgtt cgacgcgaag cagcgcgggc cggaagaacg
    60541 ggccgcgatg ctcgagcggc tcggcatcaa gcggtttgcc tacgactggc gggacaaaga
    60601 cattccgacc ttcgaccagg aaatcgacgc gctgaacaag cacgggatcc ggctacaggc
    60661 gttctggttt ccgtgcggcc tggagcccgc caaagacaag aacgccggaa tcattctgga
    60721 tctcctcgaa cgccggaagc tgaagaccga gttatggttt tcgctgagtg gtgagacgaa
    60781 ggagctcgac gcaatgccgc tggagaaaag ggtggcggag gtggcggaat cggtccaatg
    60841 ggtggcgggg agggcaggga agatcggctg ctcggtggcg ctgtacaatc acggcggctg
    60901 gttcggagaa ccggagaacc aggtcgcgat cctcaagcgc gtgaggatga aaaacgttgg
    60961 gatcgtctac aacttccacc acgggcacga ccacatcgag cggttcccgg agttgtttgc
    61021 tctcatggag ccgcatctca tggcggtgaa cctgaacggc atgcggaagg gcggtccgat
    61081 gatcctgccg atcggggacg gcgaccgaga gctcgagatg atcaaggtga tccggcgcag
    61141 ccgctatcgc ggaccaatcg gcattctcgg acaccgagaa gaactggatg cggaggaagc
    61201 gcttcggctg aacctcgagg gactcaagaa actgattccg attacgggtt cgtgagccgt
    61261 gtggtctggt gagcggcgag ctgccacttg ccgccgcttt tcacccagag gtgcagcatc
    61321 atgagccggt tgtcgaacgg ctcgcccttc gattttccct gcatccgcac gacggcatgg
    61381 gcgacggcgg cgttgccgtg cggtttcacg gtcaggctca ggtgctcgat cgagtcgtat
    61441 ttctgggcgc cgctcttgag cttcgcgagg tactcggcct ttgtttcgac tacgccggtc
    61501 gagtgggcgt aaatgagctg gtcatgaagc aacttgtcga gggcggcgta gtcgagcgcg
    61561 gcaacggcgg tggtccagct cttctcggcg gcgcgcacct cgtcctccgg cccggcggct
    61621 gccagtgtga cggccacgga caacatcaga acagcagcaa cgatcgtctt tcgcatgagg
    61681 aacctttctc cgaaacacag atcatagcgc acctacaact gccgcagacg cgaatcgtgc
    61741 caagctggag ggatgccgga gctgccggac gtcgtcgtct acattgaggc gatggacgag
    61801 cgggtgaagg ggcggagact agatcgggtg cggatcgccg gcccgtttct cctgcgcacg
    61861 gccgaaccgc cggtgcagga agcggaaggg aaaacggtcc tggaagtgcg gcgcttggga
    61921 aagaggatct gcatcggact tgaggacgac ctctggctcg ttttgcacct gatgatcgcg
    61981 gcgcgcctgc actggcgaaa gccgggtgca aaagtcgacg ggcggcgcac gccggcagcg
    62041 ttcgatttcg acggcggaag cctggtcctg acggaagcgg gcacgcagag gcgggcgtcg
    62101 ctacatgtcg tctcgggaaa ggacgggctc cgggcgcttg accccggcgg gctggaggtt
    62161 ctcgatgcgg gattcgagga ctttgcggct gcgctcacgg cgcacaatca caccctgaag
    62221 cgagcactga cggatccgag gatcttcagc gggatcggga atgcctactc cgacgaaatc
    62281 ctgcaccgag cgcggttgtc acccgtcgcg ctgacccaga atctcaccgc agacgacacg
    62341 cggcgattgt tcgcggcggt tcgggagacg ctgctcgaat ggacggaccg gctacgtgag
    62401 gggaggcgcg gcggcttccc tgaaaatgtc acggcattcc gggaagagat ggccgtacac
    62461 ggcaagtacg ggaagccgtg cccgcgttgc ggcgcgaagg tacagcgcat ccggtacgcg
    62521 agcaacgaaa cgaactactg cgcggtgtgc cagacgggag gaaggctgct cgcagaccgg
    62581 tcgctgtcgc ggctgctgaa gcaggactgg ccgcggacgc tggaggaact ggagggacta
    62641 aaagggaggt agcggcgacg gaagcacatc gccgccccct ttggggttac caccgaagct
    62701 tgaggttggc ctcccacttt tcgttggagc gcatcatttc gtcccaggtg atctcgcgct
    62761 gctggtaggc ggcggtgcgt ccgaggatgg cggtgagatt gctgtcgaca gaatccgcga
    62821 cgttattgac cggagtcgcg gtgcgaatac tctcgatgaa ggtcttcacg ttctcgatcg
    62881 ctccaccgcg gaaggtgtcg tctttgtccg tgccgtgcca ctcattgtca ccggtgacgc
    62941 ggatgagccc gttgtagtgc gtgtcggcgg cccccttgat tccgaagacg cggacgcaca
    63001 ggtcggcgaa gctgcgcgtt aattgatgcg agctgaagtt gacgtgtacg ttgttcggat
    63061 accaataggt gaccacgaag tggtcccagg cgtcgccggc gtcgtacgga gtgcccgacc
    63121 agtctgttcg cccgcccgtt ccggacgcct tgagcgggtg tccttgaagg agccagttgg
    63181 ccacgtcgat gacgtggatg ttctgctcga caatgatgtc gccgccgagg acgcgatcca
    63241 tgtagaagtt catgatgcgc gccaggccgg gatccatacc gggtttggaa cggtcctgga
    63301 tgacgcggcc ggcgtagtag aaaacctggg ccatggccgg agtgccgatg tcgccacggt
    63361 gcacgcgggc cgcgacggcc tggtacacgg cgcgggcgcg agtctggaag tcgacaagga
    63421 aactcaattt cgccttggct gcctttttcc cgctatcgag gatggtacgg cagccgggca
    63481 catcgaccgc gatgggcttg gcgcaaaaca cgtgcttgcc ggcatcgacc gaggcggcgg
    63541 cctgttcggg gtggtaatag gtcggggttt cgatgacgac gccgtccagc ttcgagttgg
    63601 cgagttcctt gtaagcgtcg ggaccgtagt acgcgcgcga ggcatcgacc ttgaacttgt
    63661 cgcgggtctt gtcgagatgg tcacggatga cgtcggcgag tgcgacgacg cgggcgccgg
    63721 cgaactccgg aaagaattcg ccgatccagt tcccgcggcc gccgcagccg atgagaccga
    63781 cctcgacggc ggagttagcc tgagagccga aggcggtctc tggtttcagg ataagcaggc
    63841 tgccggcggc ggccttcagg aaaccgcggc gttcggtgtc agtagacgaa tgattctcca
    63901 cgatgttgtc tcctcggccg ttccgcatca cggctggtca tagccggtta gccgccttat
    63961 ccagatgttg cgatagcgga cgggtacgtc atggtcctgg agcccgagcg gttcctcagg
    64021 ctcgtgtggt gcgtacgtgc cgacgacgcg atgcgccata cgcccgatga tttcctgcct
    64081 gtgatggacc agcacgccat tgaagaacac agtgacgtag ccgggtttca caagcttctt
    64141 gttctcgaac ttcggggcct cgaacacgat gtcgtagctc tgccactcgc ccgacttacg
    64201 ggcggggttg actagcggcg gccactggcc gtagatggac ccggctccgc cgtcggcgta
    64261 ggtggggttc tcgtacgaat cgagcacctg aatttcgtat cgatccatga gcaggacgcc
    64321 gctattgcct ttccattggc tggagtctcg gctgttcgcc ggggtggtcc actcgatgtg
    64381 tagctggcag ttgccgaatc tctctttggt aaccaggtcg cccgtgccgc ttgcgatctc
    64441 catgtagccg tctcgaactt tccagcctgg ggcgacatcc ttgcccttgc ggcggccgat
    64501 ccatttcgag agatccttgc cgtcgaagag gacgatggcg tcggaggacg gcgcgccgca
    64561 cgtgcgccca ggggtaacaa cgtgcgggcg gggtcgcgcg atgtcgtgca ctttccactt
    64621 ctggcccggc aagaccggag tgtcgtcgta gccgaccggt gacgtccgct gcgccactgc
    64681 gatggtggca acagcggcga gtgtggcgag caaaaaagcg tatgggcggg tatggcgatg
    64741 aatcataacg ggttccatga taacggagcc agcgtatcaa aattcgcgat tcgccgggat
    64801 cttttttgcg gggatggtgt ccaaggagtt atgaaccccg aaatcgaacg gtggacaaac
    64861 gagttcgaaa gtgctatttt tctagcatag gtgcaataaa actagcatgg ctgagacacc
    64921 tcattccgaa ctgagccgcc gggaacgcca gatcatggac gtcctctaca agctgaagcg
    64981 ggctactgtc ggcgacgtgc tctcacagtt gcccggcaag ccgaattact cgacggtccg
    65041 ggcgcagttg cgggtccttg aggaaaaggg ccaggtgcgg cacgaagaag aggggctgcg
    65101 gtacgtgtat gcgccgacgg cggcgcggga cgccgtgcgg aaatcggccc tgaagcacct
    65161 tgttgaaacg ttctttgacg gctcgccgga gaaggtggtg gcgacgctgc tcggcggcga
    65221 gtcttcgaag ctttcgcgag aggacctgga caggctctcc ggtctaatcg agaaggcgaa
    65281 gaataagaag ggatcgacac gatgaacgcg catttcctga ctcctgattt ctggctgtcc
    65341 gtgttggcgg acggtgcgct gaagggatcg gtgatcctgg ccgcagccgc cgtgacggcg
    65401 cggttgatgt ggaggcaggc cgccgcgcta cggcacctgg tatgggcggc ggcagtagtg
    65461 agcctgctga cgcttccagc agcgaaccgc ttcatgccgg cgtggtacaa cggcgcctac
    65521 gagaaagtgg cgctcctggc gggtggaatc gacgctggag ctgtcctgga gcccccggcg
    65581 gaagacccga tgctggtggc ggccggcggc gtgcaaaaaa agagcaacaa tcgcacctgg
    65641 attctgatag cgatctggac ggtcggattc ggcacagcgg tgctgacact cggggccggg
    65701 acgttgcggc tgtggcggct cgtgcgaggg gcgacgccgc tgttcgtgcg ccggtgggtg
    65761 ctgctgt
//
LOCUS       DATIFQ010000389         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2047588, whole genome shotgun sequence.
ACCESSION   DATIFQ010000389 DATIFQ010000000
VERSION     DATIFQ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047588"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="VLE22_19045"
     CDS             complement(<1..1797)
                     /locus_tag="VLE22_19045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB16557.1"
                     /translation="MLQDLRYGLRQLRRNPGFTAAAVVTLALGIAVNTIIFSAVNGFM
                     LRRPRIQDPASVVVIVSVDPAKGPEWGWDRKPVSAFDFVAWREQSSAFEDMVASDWSD
                     FALTEHGEPERLVGMRASAKYFDVLGVSAALGRTFLPDEDQPGHGQVVILSYGLWQRR
                     FGSNPKVIGEVVRLDGESYTVVGVMPRDYRLGVYGPQLWAPLVFAAENLSPAAREKRS
                     LEVLARLKSGVSVETAKAEMAELAQRSEQAYPGTSKGWSAAAMSIQHYIADEFRNGMR
                     LQMGVVLFVLLIACVNIASLQLTRGAERQREFAVRVALGASRFRLVRQLLVESLLIAL
                     AGGALGLLFASWGVDLFRRGLSSSPDVISIAHEVTIDHTVLIFTLGISVFAAILFGLA
                     PALHQTAVDLHSTLKEGGRASSQSRARHRTQSVLVTAQIALSLVLLIGAGVFIQEFLD
                     KVHDGFGIDPDQVLTANISLSSTRYNDPTKQAAFFHRAIERLEALSGVISAGATSTLV
                     PGEYVRIVTFSIEGQPAMSRTERERTDYFAISADYLRTLRIPLIRGRYFLPSDSTQAP
                     PVALVNQAFVRRYFPNEEPVGKRIRLDTSGSDR"
BASE COUNT          552 a          776 c          749 g          496 t
ORIGIN      
        1 tcggtccgag ccgctggtgt cgagccgaat gcgcttaccg acgggctcct cattcgggaa
       61 gtaacgccga acaaatgcct ggttgacgag ggccaccggc ggcgcctgcg tggagtcgga
      121 aggcaagaag tagcggccgc gaatcagcgg tattcgcagt gtgcgaagat aatcggcgct
      181 tatcgcgaag taatcggtcc gctccctctc cgtgcgtgac attgcaggct ggccctcgat
      241 gctaaacgtg acgatgcgaa cgtactcacc gggtaccagc gtactcgtgg cgcccgccga
      301 aatgacgccg gaaagtgcct ccagacgctc gatggctcgg tggaagaagg ccgcctgctt
      361 ggtaggatca ttgtagcgcg tgctcgaaag agagatgttt gccgtcaaga cctgatccgg
      421 gtcaattccg aaaccatcgt gaactttgtc gagaaattcc tggatgaaaa ctcccgctcc
      481 gatcagaagc accagcgaca gggcgatttg cgccgtcacg aggacactct gtgtccggtg
      541 gcgggccctg ctctgcgaac tcgcacggcc accttctttg agagtcgagt gaagatccac
      601 ggccgtctgg tgaagagcgg gcgccaagcc gaaaaggata gctgcgaaaa ccgatatgcc
      661 gagcgtgaag atcaggacgg tatgatcgat tgtcacttcg tgcgctatcg atatgacgtc
      721 ggggctcgag ctgagacctc tacggaacaa gtccacaccc caggaagcaa acagcaggcc
      781 cagcgcgccg ccagccaggg cgatcaggag gctctcgacc aacaattggc ggaccaggcg
      841 gaaacgactc gcgcccaggg ccacgcgcac ggcgaactct ctttggcgct cggcgccgcg
      901 tgtaagctgc aggctggcga tgttgacgca agcaatgagc agaacgaaga ggaccactcc
      961 catctgcagc ctcatgccat tccgaaactc atcggcaatg tagtgctgga tggacattgc
     1021 cgcggcgctc caacccttgc tcgtgccagg gtaagcttgc tcggagcgtt gggcgagctc
     1081 cgccatctca gcctttgccg tctcaacgct gaccccggat ttcaagcgag ccaagacctc
     1141 caggctgcgt ttctcccggg ctgccgggga aaggttctcc gccgcgaaga cgagcggcgc
     1201 ccacaattgc gggccgtaga ctcccagccg gtagtccctc ggcatgaccc ccaccacggt
     1261 gtaactctcg ccgtcgaggc gcactacctc cccgatcacc ttggggttgg agccgaaccg
     1321 acgttgccat aatccatagc ttaggatgac gacttggccg tggcccggct ggtcctcatc
     1381 gggcaggaag gtccgcccca aagcagccga cacgcccagg acatcgaaat attttgcgga
     1441 agcgcgcatc cccacgagcc gctccggctc accgtgttcc gtaagagcga aatcgctcca
     1501 atcgctcgca accatatcct cgaaggcact gctctgctcc cgccaggcaa caaaatcaaa
     1561 ggccgaaaca ggcttgcggt cccatcccca ttccggcccc ttggctgggt caacggatac
     1621 gatcacaacc acactggcgg ggtcttggat gcgcgggcgc cgcaacataa agccgttcac
     1681 cgcgctaaag atgattgtat tgactgcgat tccgagcgcc agtgtcacga cggctgccgc
     1741 cgtgaagcct ggattccgcc tcagttgtct caatccgtaa cgtaggtctt gaagcatgaa
     1801 ggagccctct agatcgaaat ttccggtgta atgaaagccc cacgaaggat tgttccagac
     1861 gtgggagggt agccacggcg aggtagacca ttcgccgtag cttaccaacc agctcagccg
     1921 catcccttga gacacgtttg cagagttcgt ctcacattcg ctaggcaggc ccccacctga
     1981 ccaggagcca gggtttgaca ctgctttgcg gcagcttcgg ccgcattcaa acaggtctgc
     2041 tgacaggtcg gcatagccat cgccagttgg gagcagaacg ccaaagcgat tatcagcctt
     2101 ctaaatttca tgcacgatcc tcattttcgt cgccatacta gaatgttatt ctcacagcaa
     2161 ttttacctcc acggagataa gacccttaaa agcaagaaac tgcgaaacga tgacatccgg
     2221 agtagatcag gctgggcggt tttgggattc gcttgtccga atccgcgggt gtagaggggc
     2281 gtcttgagaa cgggcttcta tccacgtact tttctcccac gcaaacgggc atttgaaagc
     2341 ggtccgttcg tccaccgtcc aattccacag gcggcaagcc agttgggcgg acggaccgct
     2401 attttttcga gtttcgcggg tcgcgtggct ttcccgcgag agggcggcga gttcgacagc
     2461 cttgtcggcg atgacctgtg cgtaggtgcg tccttcaggg tcgtcaggaa aaggtcgtgc
     2521 aagcgatcgc tcgtagcagg tactcaactt gaccgtgccc ttgggcctgc cgc
//
LOCUS       DATIFQ010000390         5013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2622348, whole genome shotgun sequence.
ACCESSION   DATIFQ010000390 DATIFQ010000000
VERSION     DATIFQ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622348"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1812)
                     /locus_tag="VLE22_19050"
     CDS             complement(<1..1812)
                     /locus_tag="VLE22_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16558.1"
                     /translation="MLFPRSRATLLVLSLIGALPLMSGAPLEQRRIYIAPDDHTDYVW
                     SADEETYRQAFLETLDYYLDQADKTASNAPERQSRWNCDGSLWMWIYEKNKTPAQFER
                     FLRRVRDGHISVPLNTLVSCYGGTPLEAILRGMYYAGRVERRHGIRFPLAVAMENQTL
                     PYGLGALFAGSGARYSWRGICGCASRLAPIRHTLRPHEIYWWQGPDNSRILMKWNSLL
                     REGETANKCVGGYAEAFDPAASLRFVETDPRFQSAWPYRVIGIFGKGWDRLKTLDQEV
                     VAAARETTTPARKVIVSNEEDFFRDFETTYGKQLPAFSAAFGNEWDLYTASVAEVSAR
                     VRRSVEKLRTAEALATLVSLKRPTFLNGRESTRDLAFLNLGLFWEHNWTSDGPVTRSA
                     RAEWGRKLTGEIEDYAGRLESDAAYALGALIRRQGSNPRFYVFNPLSWTRTDVVDLPY
                     EGPPEVRVVDLEGGVELPSQVVTTDWRTYAKGRRRLRVLAADVPPVGYRVLEIRPGAP
                     RTLSGSPTVRDRVLENAFYRLAVDPNGAITSLIDKTRGNREFARTIAGRSINDLGPSE
                     GELEIENAGPVSVTLKAQAPAPLARTTRITLYRDVNRI"
     gene            1970..2443
                     /locus_tag="VLE22_19055"
     CDS             1970..2443
                     /locus_tag="VLE22_19055"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSB16559.1"
                     /translation="MATIRFTINGKTRSVDTEPDRPLLEVVREDLALTGTKYGCGEGQ
                     CGACTVLLDGKPVFSCLTPVRAAEGRKIVTIEGLAEGGKLHHVQQAFLDEGAMQCGYC
                     TPGIVLRTVALLDGHPKPTEEQVIDGLNGSLCRCSGYHRILAAVRRAAESADSKG"
     gene            2453..4588
                     /locus_tag="VLE22_19060"
     CDS             2453..4588
                     /locus_tag="VLE22_19060"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HSB16560.1"
                     /translation="MSTHPNRKEPSEPERYELQEGPAYIFAVNRREFFGIAGAGLLII
                     ATAPSTEAQRGGESGSLEARLHIAEDGVITILTGKIEEGQGPRTELSMAAAEELRVPL
                     GRVRMLMADTDRTPNDGITAGSGSTPRTVPLVRKASAAARGLLLAAAGRQWDVDRTRL
                     EVRDGAVTHADKRYTYAELARSPELAAAYKDALPADIAVTDARDWRILGTPHPRLDGR
                     EIVTGSHQYPSDIVRPEMLYGRVLRAPSYGATLAGADLTAARKMPGVTAVHDGAFVGC
                     AAPTTYAARKALEAISATAQWKRAEHPSSDTLFEYLKQHAKEQGRVRAQARGSVEQGL
                     AEAKRRLKATYRTAYVQHAPMEPRAAVAEWQDGRLTVWTGTSNPFSVRQQLAETFRMT
                     PDRVRVIVPDMGGGFGGKHTGEAAIEAARLAKEAGRPVKLQWTRAEEFMWAYFRPAAL
                     IEVEAGLDAGGTLAAWDFTNYNSGGSALDTPYRIANTRVRFVESEAPLRQGSYRCLAA
                     TANNFAREAFTDELAEAAGKDPLEFRLAHLDNERIKNVLVTAAERFGWAERRKKRRTG
                     AGIGLACGTEKNSVVAACVEVEVDHASGVPRLIEIVEAFECGAILNPANLRSQVEGCI
                     MMGLGPALREEIQFKDGRLTNGRFASYRAPRFRDVPKADVILLDRKDLEPAGAGETPI
                     IAVAPAIANAVFDVTGKRVRSMPIRVPPG"
     gene            4790..>5013
                     /locus_tag="VLE22_19065"
     CDS             4790..>5013
                     /locus_tag="VLE22_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16561.1"
                     /translation="MLVGKDVKPGTKAELSSVLVEDMKDGGMGSIRFLTPGHAARHFG
                     KAIAQAEYVDDDGVLVSIVVNIDQENELYEV"
BASE COUNT          953 a         1494 c         1704 g          862 t
ORIGIN      
        1 gatccggttc acatcgcggt agagtgtgat cctcgtggtt cgggccaagg gtgcaggagc
       61 ttgcgcctta agagtgaccg acacgggtcc agcgttttcg atctccaact cgccctcaga
      121 cggtccgagg tcgttgatcg agcggccggc gatggtccgg gcgaactcgc ggtttccgcg
      181 cgtcttgtcg atgaggctgg tgatggctcc gttggggtcc accgccagcc ggtaaaaagc
      241 attctccaac acccgatccc ggaccgtggg agagccggac aatgttcgcg gcgcgccggg
      301 gcggatttcc agtactcggt aaccgaccgg agggacgtcg gccgccaaca cccgcagccg
      361 gcggcggcct ttggcatagg ttctccagtc ggtcgtgacc acttgcgatg gaagttcgac
      421 gccgccctcc aggtcgacga cgcggacctc cggcggtccc tcatacggca agtccaccac
      481 atcggttcgc gtccagctca gtgggttgaa cacgtagaaa cgggggttgg atccctgacg
      541 ccgaatcaaa gccccgagcg cgtaggcggc gtcggattcc aaccggccgg cgtagtcctc
      601 gatttccccg gtcagcttgc gaccccactc ggcgcgcgcg gaacgcgtga cagggccgtc
      661 gctggtccag ttgtgctccc agaagagccc gagattaagg aatgccaggt cccgtgtgct
      721 ctcgcggcca ttgagaaacg tcgggcgctt cagacttacc agggtcgcca gcgcctctgc
      781 agtgcgcagt ttctcgacgg atcggcggac gcgcgccgat acctccgcca ccgaggcggt
      841 gtacaggtcc cactcgttgc cgaaggcggc cgagaacgcc ggcagttgct ttccgtacgt
      901 cgtttcgaaa tcacggaaga aatcctcctc gttcgaaacg atcactttgc gcgccggcgt
      961 ggtcgtttcc cgcgctgcgg ctactacctc ctgatcgagc gtcttaaggc ggtcccaacc
     1021 cttcccgaag atgccgatca cacggtaagg ccatgcggat tgaaatcgcg ggtcggtttc
     1081 cacgaagcgc agtgaggcag cgggatcgaa ggcttcggcg tacccgccta cgcatttgtt
     1141 ggctgtctcg ccttcccgaa gcagggagtt ccacttcatg agaatgcggc tgttatccgg
     1201 cccctgccac cagtagatct cgtgcgggcg cagcgtatgc cggatgggag ccaggcggct
     1261 ggcgcatccg caaatgccgc gccaactgta gcgggcgccg gagccggcga agagcgcccc
     1321 cagcccgtag ggcagtgtct ggttctccat ggcgacggcg agcgggaaac gaatgccgtg
     1381 gcggcgctcc acgcggccgg cgtagtacat tccgcggaga atggcctcca gaggcgttcc
     1441 gccatagcag gacacgagcg tgtttaacgg cacgctgatg tgcccgtcgc gcacgcgccg
     1501 cagaaaacgc tcgaactggg ccggcgtttt gtttttctcg tagatccaca tccataggct
     1561 gccgtcgcag ttccagcggc tttggcgttc aggtgcgttc gacgcggtct tgtcagcctg
     1621 gtccagatag taatcgagcg tttccaggaa agcctgccga tatgtctcct cgtcggctga
     1681 ccagacataa tcggtgtggt cgtccggggc gatgtagatg cggcgctgct ccagcggggc
     1741 gccggacatg agcggcagcg cgccgatcag cgacaggaca aggagcgttg ctctactccg
     1801 cgggaacaac atcggctctc tcccgtgggg cccggcaggg cccgctgtgt gcacgtcaaa
     1861 gcatatcaag gttcccccta cgtcgaaccg tcggtctaca ctggttgcta gcggcgtgtg
     1921 tggcagcgct gcttgctgtt ctgggacgat cgcggggagg tcacaaagaa tggcgacgat
     1981 ccgattcaca atcaatggaa aaacacgcag cgtggacacg gagcccgacc ggcccctgct
     2041 ggaagtcgtg cgcgaagact tggcgttgac gggaacgaaa tatggctgcg gtgaaggcca
     2101 atgcggcgcc tgcaccgtgc tgctcgatgg taagccggtc ttctcctgcc tgacaccggt
     2161 gcgcgcagcc gagggccgga agatcgtcac tatcgaaggt ctggccgaag gcggcaagct
     2221 tcaccacgtg cagcaggcgt tccttgatga gggcgccatg cagtgcggct actgcacgcc
     2281 gggcatcgtc cttcggaccg tggcactgct ggacggccat cccaagccca ccgaggagca
     2341 agtcatcgac ggactgaacg ggagcctgtg ccgctgttcc gggtaccaca ggatactggc
     2401 cgcggtgcgg cgcgccgctg agtccgcgga tagcaaaggc tgaggcgcga ccatgtcaac
     2461 tcatccgaat cgcaaagaac cgtccgaacc ggaacgctat gaattgcagg aaggacccgc
     2521 ttacatcttc gccgtgaacc gcagagagtt cttcgggatc gccggtgcgg gtcttctgat
     2581 tatcgcgacg gcaccgtcca cggaagccca gaggggaggc gaaagtggtt ccctcgaagc
     2641 caggctgcac attgcggagg atggcgtcat tacgatcctg accgggaaga tcgaggaggg
     2701 ccagggtccg cggacggagc tgagcatggc cgcggccgaa gaactgcgag tgcctctggg
     2761 ccgcgtgcgg atgctcatgg cggatacgga ccggaccccg aacgacggaa tcaccgccgg
     2821 gagcggttcg acacccagga cggtgccact ggtgcggaaa gcgtcggcag cggcacgcgg
     2881 gttgctgctc gccgcggccg gccggcagtg ggatgtcgat cgcacgcggc tcgaggtgcg
     2941 cgacggcgcc gtcacgcacg cggacaagag gtacacgtac gcagaacttg cacggtcgcc
     3001 ggaactggca gcggcctaca aagacgcgtt gcccgcggac atagcggtca cggacgccag
     3061 agactggcgc atattgggga cgcctcatcc ccggctggac ggccgtgaga tcgtcactgg
     3121 ctcgcatcaa tacccttccg atatcgtgcg gcccgaaatg ctctacggcc gggtgctgcg
     3181 ggcgccctct tacggggcga cgctggcagg cgcggatctc acggcggccc ggaagatgcc
     3241 gggcgtgacc gcagtgcacg acggcgcttt cgtcggatgc gccgcgccca caacgtacgc
     3301 cgcccgaaag gcgttggagg cgatctcggc cacggcgcaa tggaagaggg ctgagcatcc
     3361 atcaagcgat accctcttcg agtacctgaa gcagcacgcc aaggagcaag gacgcgtccg
     3421 ggcgcaggcc aggggttcag tggaacaagg cctggcggag gccaagcgca ggctgaaggc
     3481 cacttaccgg accgcctacg tgcagcacgc ccctatggaa cctcgcgctg cggtggcgga
     3541 gtggcaggac gggcgtctga cggtatggac cggaaccagc aatcctttca gcgtccggca
     3601 gcagcttgcc gagacgttcc ggatgacgcc cgatcgggtg cgcgtgattg tccccgacat
     3661 gggtggaggt ttcggcggca aacataccgg tgaagcagcc attgaggctg cgcggctggc
     3721 gaaggaagcg ggtcgccctg taaagctgca atggacgcgc gcggaagagt tcatgtgggc
     3781 gtacttccgc ccggctgcgc tgatcgaagt ggaagcggga ctggacgcgg gcggcacttt
     3841 ggcagcctgg gatttcacga actacaactc gggagggtcg gcgctggaca ccccctatcg
     3901 catcgcgaat acacgggtga ggttcgtcga atccgaggca ccgctcagac agggctcata
     3961 ccggtgcctg gcggcgacgg caaacaactt cgcgcgggag gcgttcaccg acgagctggc
     4021 cgaagcggcg ggcaaggacc cgttggagtt caggctcgcg cacctcgata atgagcggat
     4081 caagaacgtc ctggtcacgg ccgctgagcg gttcggctgg gcggaacgcc ggaagaagcg
     4141 ccgaacgggc gcgggtatcg gcctggcctg cggaaccgag aagaactcgg tggtggcagc
     4201 ctgcgtcgag gtggaggtgg atcacgcgag cggggtcccg agattgattg agattgtcga
     4261 ggccttcgaa tgcggagcga tcctgaatcc ggcgaacctg cggtctcagg tggaaggctg
     4321 catcatgatg ggcctcggcc cggcgctgcg ggaagagatc cagttcaagg acgggcggtt
     4381 gaccaacgga agattcgcga gctaccgtgc gcctcggttc cgggatgttc ctaaagcgga
     4441 tgtgatcctg ttggaccgca aggacctcga gccggcaggc gcgggcgaga ctccgatcat
     4501 cgccgtcgct ccggccatcg cgaatgcggt attcgacgtg accggcaagc gcgtgcgctc
     4561 gatgcccatc cgtgttccgc ccggctaagc gtgcgtagcg atctcagcgc tgcggaactg
     4621 caaggcctcc ctgggttctg tctcggcacg tgaatgtcgg tggtaaggta tggacgtata
     4681 gcgcctacaa ggtcgacgga tggcacgatc aatgttagaa ggatcacagt caaataatcc
     4741 tccacagggg cgaccgctgc gtccccttga acgcgagctt gtcgagagaa tgctggtcgg
     4801 gaaggatgtc aaaccgggca cgaaggcgga gttgtcttca gttctggtag aagacatgaa
     4861 ggacggcggg atggggagta ttcgtttctt gacccctggc catgcggcgc gtcactttgg
     4921 aaaggcgata gcacaggcag aatacgtcga cgatgacggt gtgctcgtga gcattgtagt
     4981 taacatcgac caggaaaatg aactatatga agt
//
LOCUS       DATIFQ010000391         9962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_706785, whole genome shotgun sequence.
ACCESSION   DATIFQ010000391 DATIFQ010000000
VERSION     DATIFQ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9962
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706785"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VLE22_19070"
     CDS             <1..544
                     /locus_tag="VLE22_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16562.1"
                     /translation="GGEVFANIQGRWQDVEPAGARSVAERYAAYDSTRDGKCAATVNR
                     VGSGEIAAIYGPAGSVFAAAHTPAAREFLRGVVAQVFRPRVRLEGPPTVELSLRRKDG
                     KLLVHLGNCTGMQVAGEYAAVDFVPAVGPLGLSIDLPAQPKRVTLEPGGRELRGDWLN
                     GAWTGTLDRLEIHSIVAVEV"
     gene            582..658
                     /locus_tag="VLE22_19075"
     tRNA            582..658
                     /locus_tag="VLE22_19075"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:616..618,aa:Val,seq:gac)
     gene            1133..1465
                     /locus_tag="VLE22_19080"
     CDS             1133..1465
                     /locus_tag="VLE22_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16563.1"
                     /translation="MPKTKLADMKSEAEEARWFEENQDRLRKLFEQAEKEGALRIRGR
                     SVGITFSKKTEAVQKPPSQKVMLRIPTDDVDRARRQAAGKGIGYQTYIKMLLRAGLDR
                     EEKIRRRA"
     gene            2237..2536
                     /locus_tag="VLE22_19085"
     CDS             2237..2536
                     /locus_tag="VLE22_19085"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HSB16564.1"
                     /translation="MEQIRVVYEEMKVLGKELVDKVGQLIHAGNVRRIIVKDDKGNTF
                     MEIPLTLATVGVIAAPVLAGLGAIAALIANFTLGVERVKEDTTEVGPKDAQSGDA"
     gene            complement(2951..3577)
                     /locus_tag="VLE22_19090"
     CDS             complement(2951..3577)
                     /locus_tag="VLE22_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16565.1"
                     /translation="MPNDESRSELRPLLPELIRLQQNIRDLEAKKTAIEERIMTGLFG
                     SPYSASEIPAENGDDELTEGPELAEKLTPRLVKSAEKERAVVQFAGTVMDALVKYFAV
                     AEELILSESGKIRAALRQEVEAELRDNLERSVQSLSTRLEQQQEEQSSLRALTNTAVE
                     RLNRHTLAIRSVSDVQVRHASTLRQIAEALKRLAALAETPLPQMNAGL"
     gene            complement(4348..4524)
                     /locus_tag="VLE22_19095"
     CDS             complement(4348..4524)
                     /locus_tag="VLE22_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16566.1"
                     /translation="MKFSTTNPIYWGIGGMLVSYGLSKPTLERRVLAAAGLFVLAIKA
                     AEGLSSLARTIPSK"
     gene            complement(4536..5150)
                     /locus_tag="VLE22_19100"
     CDS             complement(4536..5150)
                     /locus_tag="VLE22_19100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HSB16567.1"
                     /translation="MRDQLLRRLEVVLYVAGLACLATLLWTVSERAWFQWRYRGADSM
                     PAPSGAASPRRGPLGRIEIPRVGVSAVIFDGVEDRTLRRGVGHVPGTAYPGETGERIA
                     LAAHRDTFFRNLRLLRTGDMVRLVTHDITETYRVETTKVVAPWRTDWIQPAREDVLTL
                     VTCFPFSYVGNAPERFVVQARRIARNETMTRNQARPSPSRRVSP"
     gene            complement(5143..5955)
                     /locus_tag="VLE22_19105"
     CDS             complement(5143..5955)
                     /locus_tag="VLE22_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16568.1"
                     /translation="MRKNLVRIFSVPMAVLALALVLTPAAAPDVTDKQTELTFSAPVE
                     FPGRVVVPAGDYVMKLMDSSSNRNIVQVLNADRDHVYATFIAIPAQRLEPSDKTVLTF
                     YETTSTDPAFIHKWFYPGDTFGQEFVYPKDRARYIASAAHTSVPAVSDESGAITRVTP
                     NEDREVTQEAMNRAPALPGEPAQENPPPAPPQTTKPAASEPEKPASAQPMNSATETQS
                     PPAAGEQLPATASDASEIALAGVLFVTAALLLTLSRTAPRKRRGQPSGMPNA"
     gene            6344..7339
                     /locus_tag="VLE22_19110"
     CDS             6344..7339
                     /locus_tag="VLE22_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16569.1"
                     /translation="MPRLSSIEVEDERTVPPAHHHLGRARDWDEEPPEPRKSRLGFWL
                     AMFCLAGALLGGAWYFYPRLEAYRSQLNGLPETLSAIRERMGAAEQRLGDLPAQIELL
                     KERAAGASQRLEQKLNSGLEQARKGTRDLAVSMRREMLEAIGAQEKTVALRFKEVEAR
                     QQAEAARAAQLEQQVTQLNQRMAAMIEDVNNARTAGARESQELREQIRQTDGRVAQVA
                     SFNDRPRERFEVSRGKTQQIGPGIFLHVSGVNPRYRRYHGWLQLVNDGKILWLRDQSV
                     LQTVAFYAGEKGLRHDLLVTGLTEGGVAGYVIFPSVGESGGAPKTGTPAGMSIGQ"
     gene            7437..>9962
                     /locus_tag="VLE22_19115"
     CDS             7437..>9962
                     /locus_tag="VLE22_19115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB16570.1"
                     /translation="MRLRSAISLSVSLCLLIALAAMPLAAQTGLGVVRGTALDATKAA
                     IPSAKVTLTNTETGISRTTETSSVGIYYFGATPIGSYTLTVEAPGFKKWSGTLKVEAG
                     QTVVVDPAMEVGTLEATVEVTGAAPVVTTEGAQVSNVKDALRIHQLPLNGRAVTTLFN
                     LTPGVEGGGSPRVNGMKVGSTEMLLDGISLVDRFGGGISRVQPGLDTIQEFRIETAGS
                     GAQYSRPATVSLVTKSGTNAIHGSVFETHRNNFGGLRARQRQDGNDAAQLIRNEFGVS
                     AGGPVIKNKTFWFFAYEATRNRQSNFARTSTPTPAMWDGDLSSAITPDELPVTVYNPF
                     STAADGSRIPFINNRVPTNYITPFAQTMRSITPEPTGSGNPWLEPNFETYYPDQSDIN
                     TYTIKGDHVFSERDNISGRFTRSAQSSKLFGGRFGFPPPGCTNCGGSGLTDAKVYSTF
                     ARWNHVFSPTFLNELQASNHYSPKSSGTLGNDVNWADKLGLPNPFGVTGWPTICMSDY
                     FLYYGCWDGDNRKDEHLTAFQIDDNVTWIKGKHSIKAGFKGRQEYNNIRELQQAQGSH
                     SFYQNWTALYDPINDDETPLTGSGLAGVLMGLPTFLSNQYNRGYFYFQQKEIGLYFQD
                     TWKLTPRVTLDLGLRWDKWTPYHEKYNRLVNVDLQNYANQFQVITPHNVKMEDIPGVP
                     PAVLQSWAGRGLTWVTADSIGFPGSLVPADNNNFGPRLGVAVRLTNRWVLRGGYGRYF
                     WTMPLAQILQSSRGNPPLNLRFANRIASANNADPFHADRSVPSSDEFIGKATVDITGV
                     QSIGSGAQSIMPWDVSDWTDNQADEWTFTLERELMKDTALRLSY"
BASE COUNT         2034 a         2893 c         3083 g         1952 t
ORIGIN      
        1 gggcggggaa gtgttcgcga acattcaggg gcgctggcag gacgtggagc ctgcgggcgc
       61 gcggtccgtg gccgagcggt atgcggcgta cgattcgacg cgggacggca agtgcgcggc
      121 gacggtgaac cgcgtgggga gtggcgagat cgcggcgatt tacggcccgg cggggtcggt
      181 gttcgcggcg gcgcacacgc cggcggcgcg ggaattcctc cgcggcgtgg tggcgcaggt
      241 gttccggccg cgtgtgcggc tggagggtcc gccaacggtg gagctgtcgc tgaggaggaa
      301 ggatggaaaa ctgctggtgc atctcggcaa ctgcaccggg atgcaagtgg cgggcgagta
      361 cgcggcggtg gacttcgtgc cggcggtggg gccgctgggc ctgagtatcg atctgccggc
      421 gcagccgaag cgggtgacgc tcgaaccggg cggccgcgag ctgcgcggcg attggctcaa
      481 cggcgcgtgg actggcacgt tggaccgcct ggagatccac tcgatcgtgg ctgtggaggt
      541 gtgacgctgt gctacaattg gtggtcgtta cccaccaaaa cgggcccgta gctcagttgg
      601 ttagagcgct accttgacac ggtagaggtc acagattcga gttctgtcgg gcccaccact
      661 tgacaccaag aagctgcgac ccgcgtctgc gtaaactacg ctttgactat tttgaagcgg
      721 attcctgaac agtggttccg catcccccgg tgagtctgag tgcatcccgt cagggattct
      781 ctggcctcgg cgcgtgcctc agttgttatg tatggacaaa gggtatacac ttgagataga
      841 tccccggtgc aaacctacct gctttcgatt gggatgacgg taatagtgct catatagcca
      901 ggcatggcgt cacgcgggat gaggcagagc aggtcgtgct gggagcgtcg ctgccactct
      961 cgaacggagg agcggagcgg tgaggaacgg cacactgagc tgggcgaaac aatcgatggg
     1021 cgattgctgc tggtcgtctg gacttggagg cgccgaagga ttcgcgtggt gaccgcgttt
     1081 cctgcaaatc ggaagtggcg cacgatatgg aaacgcatca agggggaaca cgatgcctaa
     1141 gacgaagctg gcggacatga agagcgaggc ggaagaggcc cgctggtttg aagaaaacca
     1201 ggatcggttg cggaaactgt ttgagcaggc cgagaaggaa ggtgcactgc gcatccgtgg
     1261 caggagcgtc ggcatcacat tttcgaagaa gacagaggcc gtgcagaagc cgccgtcgca
     1321 gaaggtcatg ctacgaatcc caaccgacga cgttgaccgg gcgcgacgcc aggcggccgg
     1381 caaagggatc ggctaccaga cctacatcaa gatgcttctg cgagccggac tggaccggga
     1441 ggagaaaatc cggcgcagag cgtaggccct agcgccggcg ggccctgcgg gaccttgttt
     1501 tctatggcgc ctctcaggat tgcgttgaaa ggaagctccg gcaaaagaca cggattctca
     1561 cgttattgac agacgtgcga cataggtgtt tccgacagct ctcaaggtgt catttctcgt
     1621 gatgaagatg aagttgcttt atttcttcca acttgcttaa tctcacaatc ttggccccag
     1681 agcggtaaat gggaggtaag atgcgccggg cctcctcctt gctctcaacc tccacaatga
     1741 tccagctcgt gtgttccccg tctaagcatc caagatccgc ctgcgtcagg tagtgggaac
     1801 ctgtcgagag caggacttgg gcagctcgca aacatgccac tggttccgcc tcatgcggca
     1861 attcaatcag aaatctcgcc attgcacgag cagcctttcc ccttcagtca tccctcggct
     1921 gtagttccga cgtgcaacca tccggccggg aggtactccg ccagaatttc cgtttggctt
     1981 cttgccgcca tccattgtac atgcatccgg cttggtcgga gccgcattct tcattcccaa
     2041 cgtccccagc gacttcgacg gacccacggg cggcagccgc gcctcctacg ggtagtggaa
     2101 aaccaggaag tgcctcattt aaagctattc cttgggaccg gcccggcggc cgttggtaac
     2161 ccggcgaacc gggcgcgggg aacgaaagca ccgccggaaa ccttggtttc gacgactctg
     2221 ctaccctggt cccatcatgg agcagatccg cgtggtctac gaagaaatga aggtcctcgg
     2281 gaaggaactg gtggacaagg tgggccagtt gatccacgcc gggaacgtgc gccggatcat
     2341 cgtcaaggac gacaagggga acacgttcat ggaaatcccc ttgacgctgg cgacggtggg
     2401 cgtgatcgcc gcgccggtcc tggcggggct gggcgcaatc gccgcgttga tcgccaactt
     2461 cactcttggc gtggagaggg tgaaggagga tacaaccgaa gtcgggccca aagacgcaca
     2521 atccggcgac gcctgagcag gaggcagagc gcgtgctccg actcctgcgg gaagggctgg
     2581 gatcgagcgg cgcttggtgc acgtccagaa ggcgtcctga gcccggcgca cgaatttgga
     2641 gtctaaaagc ggatcgcctg gcacgccaaa ccctgcacgg ccaagcctag aaagaccggc
     2701 ccagagtgac aaatcgcggt ctcttattgc ctgaacgggg gagcaggccg atctgatggc
     2761 ggagcgccag atcctccagt tgctgtaact ccttgccggg acagtgtgtc caggaagtgg
     2821 cgacatgacg gccggcactg gagagagaaa gaaagcaacg gaactggtat ttgctgggat
     2881 tgacggaagt acaacgtgtc gacaactagc aggttggata catcggctgt tcacctttgg
     2941 tggaatcgga ctagagtccg gcgttcattt gcgggagggg cgtttctgcc agggcggcta
     3001 atcgcttcag agcctctgcg atttgacgca gagtactagc gtgcctcact tgcacgtcac
     3061 tgactgagcg aatggccaaa gtgtgtcgat tgagccgctc cacggctgtg ttggtcaacg
     3121 cccgcaagga cgattgctcc tcctgctgct gttcaagccg ggtggagagc gactggactg
     3181 agcgttctag gttgtcccgc aattccgctt cgacttcttg ccggagcgca gcacggatct
     3241 tgccgctctc actgaggatt agctcctcgg cgacggcgaa gtactttacc aaagcgtcca
     3301 tcacggtgcc tgcaaactgg accactgccc gctccttctc tgcggatttc acaagtcgag
     3361 gagtaagctt ctcagcaagc tcgggacctt cagtcagttc atcgtcaccg ttctcggccg
     3421 gaatttcact cgcactgtaa ggggatccaa agagacctgt catgatccgc tcctcgatgg
     3481 ccgtcttctt tgcctctaga tccctgatat tttgctggag tcggatcagt tcgggcaaga
     3541 gtggtcttaa ttcagatctg gattcgtcgt tcggcatagc gactccaatt acgggctttt
     3601 ccgcgcaggg tgcggacccg gcgtcaagag cccgttctag agaacaaccc gtgagaacca
     3661 acagtgcgga actcatggtg cgtgatggag accacagtcg ttttgggcgc gtgacggacc
     3721 acacgcccga agatgacgag cttaagaggg gtccagcctc taagccggac cggccagtca
     3781 attgtcagtt cgaccgaacg accaagcggc ggaggactct caattgtgaa ggacaccccg
     3841 gtgctgcgga tgtcgacggt cctgccgaat ctgggtaccc cagtttctga tttgcagcga
     3901 actgctctct caatgtggaa tcgggtcgaa ccccggcgct ctttgaaagc gcctgctgtc
     3961 tcggtgccgg gcaaaacctc agcggctgac atggaacctc ctccgcagca ctatgctgct
     4021 cgcatcctcc atccgtatgc cgatctcagt cgccaactca cggcagaggc tacgctcgct
     4081 tgtctagggg ggaggttggc cattatatgc tcaaactgat atcagaagtc aatggcttgt
     4141 tgcgattcaa gtgtaatgag tgcccggatt gggcagccgt gaaacggtgc ggacgtggcg
     4201 gatgggctcc ggcgggcagc cttcaaacgg cgcactccgt gcctcatgga cgccgctgcg
     4261 gggcaaggcg gagacggctg acctgctgag gctgtcggtc cttcgtctcc acgggcgttt
     4321 tccgtgcgga ctcaggcaac cagctcatca cttcgacggt atcgttcggg cgagcgagga
     4381 aaggccctcg gcggctttga tggccaggac gaagaggccg gcggcggcca aaacccgacg
     4441 ctccagcgtc ggtttcgaga gtccgtagct caccagcatg ccgccgatgc cccaatagat
     4501 aggattggtc gtggaaaatt tcattactta ttgcctcacg gggacacgcg ccggctgggg
     4561 ctgggtctcg cttggttccg cgtcatggtt tcgttccgcg caatgcggcg agcttgcacc
     4621 acaaaccgtt ccggagcatt tcccacgtag ctgaaaggaa aacaggtcac gagggtcaac
     4681 acgtcctcgc gggccggctg aatccaatcg gtccgccacg gcgcgacgac cttcgttgtc
     4741 tcgaccctat acgtctctgt gatatcgtgc gtaacgagtc tcaccatatc gccggttctc
     4801 aggaggcgaa ggttgcggaa gaacgtgtcg cggtgagctg cgagcgcaat gcgctcgccg
     4861 gtctcaccgg ggtacgcggt gccgggcacg tggccgacgc cgcggcgaag cgtcctgtct
     4921 tcgacgccat cgaaaatcac agctgagacg ccgacacggg gaatttcgat tcttccgagc
     4981 ggaccgcgcc ggggcgatgc ggcgccggac ggagccggca tggaatcggc tccgcggtag
     5041 cgccactgaa accacgctcg ctccgacacg gtccacaata aagtggccag gcacgccagc
     5101 ccggccacgt acaggacgac ctcaagccgc cgcaggagtt gatcacgcat tcggcattcc
     5161 ggaaggctgt cctcgccgct ttctcggggc ggtccggctc agcgtcaaca ggagtgccgc
     5221 ggtaacaaac agaacgccgg caagcgcgat ttcggaagcg tcgctcgccg tcgcaggcaa
     5281 ctgttcaccc gctgccggcg gtgactgcgt ttccgttgcc gagttcatgg gttgagcgga
     5341 agccggtttc tcgggctcgg atgcggccgg ttttgtcgtt tgaggtgggg cgggcggcgg
     5401 gttctcctgc gccggttcgc cgggcaatgc aggggcacgg ttcatcgcct cctgcgtcac
     5461 ctcccggtcc tcgttgggag ttaccctcgt gatggccccg ctctcgtcac tcacggccgg
     5521 caccgaggtg tgcgcggcac tggcaatgta acgggcgcga tctttcgggt acacgaactc
     5581 ctgtccgaag gtgtcgcccg gatagaacca cttgtgaatg aacgccgggt ctgtactcgt
     5641 tgtctcatag aaggtgagga ccgtcttgtc cgacggctcg agtcgctggg cgggtatggc
     5701 gatgaacgtc gcatacacgt ggtcccggtc cgcattcagc acctgaacga tgttccggtt
     5761 ggacgacgag tccatcagtt tcatcacata gtcaccggct gggactacca ccctgcccgg
     5821 gaattccact ggtgccgaga acgtcaactc ggtttgctta tccgtcacgt ccggcgccgc
     5881 ggctggagta agaacaagcg ctagcgcgag tacagccatc ggaaccgaaa aaatacgaac
     5941 aagattcttt ctcactgttg cacctcctgt gctggttttt tgaaggttcc actcgaacct
     6001 attgagattg agacgcggac ccgtttggcc tctctgagcg cctctctacc caacctttgg
     6061 cactcgggat tccacgggcg gaccagtcgt tccaatcaaa ggacttagcc tgtttgcgat
     6121 tcgcgtttgc ccggtaaaaa ttcccaggtc tgcccacgtc gtgcagtcac atcagcttga
     6181 cttagcgcgc cgccgaagtt tcaacgattt acggcgctgg cgggcccgaa aggtagttga
     6241 aaaaactaca cgccggctaa tcgcactgtt gttgatttct ctcgtttggc agtgctcctg
     6301 ctttcattca cttgacagcc aaggtcgcga aaggaggcag tttgtgccga gactctcttc
     6361 aattgaagtg gaagatgaac gaaccgtccc tccggctcac caccacctgg gccgcgcacg
     6421 agactgggac gaagagccac ccgagccgcg caagtcgcga ctcggtttct ggttagcgat
     6481 gttttgcctg gccggcgcac ttctgggcgg tgcgtggtac ttctacccac gcctggaggc
     6541 gtaccggtcc caactgaacg gattgcccga gactttgagc gcaattcggg aacggatggg
     6601 cgcagcggaa cagcggctcg gagacttgcc tgcgcagatc gagttgctca aggagcgtgc
     6661 ggcgggcgcg agccagcggc tggagcagaa gctgaacagc ggactggaac aagcgcggaa
     6721 aggcacgcgg gatctcgccg tctccatgcg gcgtgagatg ctcgaggcca tcggcgctca
     6781 agagaagacg gtcgcccttc gtttcaaaga agtggaagcg cggcagcagg ccgaggcagc
     6841 gcgagcggcg caactggagc agcaggtgac acagctcaac cagcgcatgg cagccatgat
     6901 cgaggacgtg aacaacgctc gaactgcggg cgcccgcgag tctcaagagc tccgcgaaca
     6961 gatccgccag acggacggac gggtggctca ggtcgccagc ttcaacgacc gcccccgtga
     7021 gcgtttcgag gtcagccggg ggaagacgca gcagatcggc ccggggattt ttcttcatgt
     7081 cagcggggtc aatccccgtt accgccggta tcacggctgg cttcagcttg ttaacgacgg
     7141 caagatcctg tggctgcggg atcagagcgt gctccagacc gtggcctttt atgcgggtga
     7201 gaaggggctg agacacgatc tgctcgtgac cgggctcacc gagggcggcg tcgccggata
     7261 cgtgattttc ccgtccgtgg gtgagtcagg aggggcgccg aagaccggaa ctccggctgg
     7321 catgtcaatt ggccagtaaa agcactaatg tgaaattctt gcgtgtcgcg caaaaacatc
     7381 cttgacagca gcaagattcc gctatacatt tgattcaact tcaggaggga tgcgcgatgc
     7441 gcctacgctc tgcgatttct ctgtctgtct ccctgtgtct tctcattgcc ctggcggcga
     7501 tgccgcttgc ggcccaaacc ggcctcgggg ttgtacgcgg aactgctctc gatgcgacca
     7561 aagcggcgat tccgagcgcg aaagtgactc tgaccaacac agaaacgggg atttcccgta
     7621 ccacggaaac gtcgtcggta gggatttact actttggtgc tacgccgatc ggcagctaca
     7681 cgttaacggt cgaagctccg gggttcaaga aatggtccgg cacgctgaag gtggaagcgg
     7741 gccaaacggt tgtggttgac ccggccatgg aggtgggcac cttggaggcg acggttgaag
     7801 ttacaggggc cgcgccagtg gtcacgacgg aaggtgcgca agtaagcaac gtcaaggatg
     7861 cccttcgtat ccaccagttg cccctaaacg gaagagccgt caccacgttg ttcaatctga
     7921 ccccaggtgt ggagggcggg ggaagcccca gagtcaacgg catgaaggta ggttcaaccg
     7981 agatgctgct ggacggcatt tcgcttgtgg accgattcgg cggcggtatt tcccgtgtgc
     8041 aaccgggact tgataccatc caggaatttc gcatcgaaac cgcaggctcg ggtgcacagt
     8101 actcgcgccc ggctacggtc tctctggtca ccaagagcgg gaccaacgcg atccacggaa
     8161 gcgttttcga gactcaccgt aacaactttg gcggcctgcg ggcgcgccag cggcaggacg
     8221 gcaacgacgc cgcccagttg atccgcaacg aattcggcgt ttcggctggc ggcccggtga
     8281 tcaagaacaa gaccttctgg tttttcgcct acgaggccac ccgcaaccgc cagagcaatt
     8341 ttgcccggac ttcgacgccg acgccagcga tgtgggatgg cgacctcagc agcgccatca
     8401 cccccgacga attacctgtc accgtctaca acccgttttc gactgcagcc gatggatccc
     8461 ggataccttt catcaacaac agggtgccga caaactacat cacacccttt gcccaaacga
     8521 tgcggagtat tactcctgag ccgaccgggt ccgggaatcc atggctggaa ccaaacttcg
     8581 agacgtatta ccccgaccaa agcgacatca acacctacac catcaagggc gaccacgtct
     8641 tctccgaacg cgacaacatc tccggccgtt ttacccggtc tgcacagtcc tccaagctct
     8701 ttggaggcag gttcggtttc ccccctccgg gctgtactaa ctgcggtggg agcggcctca
     8761 ctgatgccaa ggtctattct acgttcgccc gctggaatca cgttttctcg cctacatttc
     8821 tcaacgagtt gcaggcgtcc aaccactatt ctccaaagag ttccggaaca ctgggcaacg
     8881 acgtcaactg ggcggacaag ctaggtcttc cgaatccctt cggcgtcacc ggatggccga
     8941 ccatctgcat gagcgactac ttcctttatt acggctgctg ggatggcgac aaccgcaagg
     9001 atgaacacct cacggctttt cagatcgacg acaacgtgac ctggatcaag ggcaagcact
     9061 caatcaaggc cggctttaag gggcgccagg aatacaacaa tatccgcgag ctacaacagg
     9121 ctcagggttc ccatagtttt tatcaaaact ggacggcgtt gtacgacccg atcaacgatg
     9181 acgagacgcc tctgaccggt tcgggccttg ccggcgtatt aatgggcctg ccgacgttcc
     9241 tgtcgaacca gtacaaccgc ggctacttct acttccagca gaaggaaatc ggactgtact
     9301 ttcaggacac ctggaagctc accccaaggg tcactttgga tctcggactt cggtgggaca
     9361 agtggacccc ctaccacgaa aaatacaacc ggctggtgaa cgtcgacctg caaaactatg
     9421 ccaaccagtt ccaggtcatt acgccccaca atgtcaagat ggaggatatc ccgggagttc
     9481 ctccggccgt gcttcaatcc tgggcaggaa gaggcctcac atgggtgact gccgattcga
     9541 tcggttttcc cggcagcctc gtgccggcgg acaacaacaa tttcggtccg cgcctaggcg
     9601 tagccgtccg gctaaccaac aggtgggtcc tgcgtggcgg ctacggaagg tatttctgga
     9661 cgatgccgct cgcgcagatc ctgcagtctt cgcgaggcaa tccaccgctc aaccttaggt
     9721 tcgccaaccg cattgccagc gcgaacaatg cggatccctt ccacgctgat agaagcgtac
     9781 ccagctccga cgaattcatt gggaaggcga ccgtggacat aacgggcgtc cagtcaatcg
     9841 gttcgggcgc tcagagcatc atgccgtggg atgtcagcga ctggacggac aaccaggcgg
     9901 acgagtggac cttcactctg gagcgggaac tgatgaaaga tacggccctg cggctgtcct
     9961 ac
//
LOCUS       DATIFQ010000392        20388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_897359, whole genome shotgun sequence.
ACCESSION   DATIFQ010000392 DATIFQ010000000
VERSION     DATIFQ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 20388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20388
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897359"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="VLE22_19120"
     CDS             <1..1855
                     /locus_tag="VLE22_19120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16571.1"
                     /translation="LILIALGSSVTVGTVRGQGISFLREFNNSEARGVAVDASGIYVA
                     GGTGMSGFVRKFDLSGNHLWARVSGLGAAAIVASTGGVYVVGSDAAAGFIRKYDAEGN
                     ELWSRQTHNSIASAVAADATGVYVGDYDPAPRKDGSVRKYTADGEELWSHQLRGGPWP
                     VGVAVDASGVYVVVRDSVKKYTADGKELWTRMFWQGGAWPVAVAVETTGIYVVGNTDT
                     YPNPEQIVLVKYDSEGNELWIRQFDWLDVGGFVAPSGVAADATGVYIVGQTTRRLPDQ
                     CESSGQDVFVRKYGPNGVSLWSRRFLGSGDIGYASGVTVDTTGVYVVGSSVSNTWDGL
                     RNSSRTNALLAKLEKTQPVIGESGPWIWPGCVVNAASYLGGRISPGEIVTVFGRRIGP
                     PEATPPLHLTAGRRLATTLAGTRLLFNGIAAPLYFASANQISAIVPYAVADKPTADVE
                     VEYQGVRSNKVTIPVAASRLGVFTSDRSGSGPAAALNEDGSINSSSNPAKQGSIIVLY
                     ATGEGLTEPVVEDGVVLGSVLPKPKLPVSVSFLAPGKPCDRHDYYSCYDSYFVTGRVL
                     YAGGAPRSVAGLLQVNVRLPPDAYPDVGYTQVEFILCVGDRFVSGSLYIRR"
     gene            1859..4216
                     /locus_tag="VLE22_19125"
     CDS             1859..4216
                     /locus_tag="VLE22_19125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB16572.1"
                     /translation="MLKADSDAFARVEEAVSRKPHIAGILAGLTLAWCRSDSTAPPSS
                     ADFALYRFDDNLKTATAKAPQRPSSPRLAEPVVWKNFYTEKASTWIPRGGSMRLRNGD
                     LLLQGDGGLPVILSPKDFPIDWSLYEAVRIRMMAEAGEEVRIKIGPDEFTQKLGPPGQ
                     YQVYRFDVNIHPPKGSRPLAIVPTDSLTGLVAVDFIELVPRRAYFHEAVGRQFLGKRD
                     DYVNALYAHSPSSLTYNVRVPEHAKLRFRIGVAESKAVAFRVAAEGTELFRRTLEDPS
                     AWEDGEADLSRFAGRTIKLVFETSSAQEGAVAMWANPRMTATGARSRPNILLYLVCTM
                     RRDHMSLYGYPRDTTPFLKTLGSAGVVFEDAHAQAAWTKASVPSLMTSLSAYATGIVR
                     ENDTIPSGATTLAEQLRRAGYVTASFVTSPFAGKASGLDRGFDYVAEHRLIQRMRSEE
                     TDRGTDSAALNKVLAAWLERYWDEPFFLYAHSNDPHAPYDPPAEFEAKFANPAETAQF
                     NRDYGLLRDRHMYGGGATISRADCLRKGIHPDRFLRRAIERYDAEIAHNDSSIERLAG
                     KLKELGILDNTLIIVVSDHGEEFWEHGWTGHGHSLYQELTQVAWLMWNPKMLPSPKRI
                     GEPVQLMDLVPTVLDLLKIRPSITLKGQSLLPLLRGKTFERKTPVMASRFAHPGAKPH
                     GFVPENRTGTFALITRDWKLIWRDQARLSALPEIELYDRRTDQAETRNVSAQYPEVVR
                     RLKAEMDQWVEAQKPVRKLVGATGHTSLSPEAIERLRSLGYIGKQ"
     gene            4667..5935
                     /locus_tag="VLE22_19130"
     CDS             4667..5935
                     /locus_tag="VLE22_19130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB16573.1"
                     /translation="MTTPDTQHRRFAWLIILLLFAGSVLNYVDRAVLGVLMPQIRHDL
                     SLSYSDYSLAVNAFLVMYTILYVLGGRMADQLGYRRSFTLTLAFWSFACMLHAVTRGL
                     ASLCLFRGLLGIGEGGYYPAAMRGVADWFPPQNRAKAVGVLLCGISIGTLLTPPFVAW
                     LTAIYGWRSAFLVIGAFGFLLIPPWLALHRRIAAAYGTSGPAHAAQPAASASDGDDPS
                     IAEVLTRRKYWCILSARALTDSAWYFYLFWMPGYFQEVRHFDLRMVGALLWIPYFGAN
                     LGALGGAWASSGLIARGWSVDRARKSLLIPSAAVCTLGALTGFVPSSYVALALVSLAL
                     LGHQSWSSNIHTVITEITPPKHVAMLYGITGAAGTLMGALTQPAVGRVVDALGYEPAF
                     VAAGSAYAAAIVLLIAAGRIERIRRGRPAA"
     gene            5955..7205
                     /locus_tag="VLE22_19135"
     CDS             5955..7205
                     /locus_tag="VLE22_19135"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSB16574.1"
                     /translation="MARLLDQKATRAIDELVKAQAVQDCLQFFAGEKQWINERHIELC
                     RIPAPTFLEERRAEWMAAQFAALGWHVEADGAGNVIARPDAARTGPYIALTAHLDTVL
                     SPRNPEDIKVEPDGKFTGPGVSDNGAGLAALLAVARAWKLSCPVPDSEVRLLLVANVG
                     EEGEGNLSGMRYLCNKRPLADLIQTFVVLDGPATDHITCQALESRRFEVSFAGPGGHS
                     WSDHGIGNPVHALGRAIAFFCDLQTNGGPSGHARTSFNFGIIDGGASVNSIPTSARAK
                     LDIRSENGARMDEMCTFLSTSVQKALEVENDRASKGRVSARIREIGARPGGVLPEDSP
                     ILSYVRAADAWLGLRARLDCASTDANIPLSMSLPAISIGAGGQGGGAHTPGEWYQPEG
                     RDLGLQRILLTLCLLMREPAVTGG"
     gene            7166..8095
                     /locus_tag="VLE22_19140"
     CDS             7166..8095
                     /locus_tag="VLE22_19140"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSB16575.1"
                     /translation="MPADARTGGDWRMISPVVRPRWGADLALAGIALIWGATFVLVKQ
                     ALADVSTILFLALRFLLAAAALALALRGRYAGFLERPKALAGGVLAGLFLFSGYAFQT
                     AGLRYTTPSKSAFITGLSIVLVPLVTSLVYRTAPRILEGLGVMVAAAGLALMTLEDLS
                     VGLGYGDLLTVCCAVAFAVHIVAVGHCSRRFGFEGLSVVQIASAAVFSLATFWWMETP
                     QVRWTAGLLVALAVTGLLATALAFTVQAWAQKHTTATRTALIFALEPVFAWATSYIVA
                     GETLSNRAVTGAILILAGIVLVELKPLSGGEHP"
     gene            8167..9759
                     /locus_tag="VLE22_19145"
     CDS             8167..9759
                     /locus_tag="VLE22_19145"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSB16576.1"
                     /translation="MSLFTTIRRQKLLSLSLLLFTLSLGILIGTLVNSGVRAERGQTA
                     VDATPLTIPNPVQLSTAFNQLAKKVEPTVVNIRADYLARDTQSSRNRRGQNPQGEEGD
                     PGFDFFHRFFGGPFGQAPEMPFRREGTGSGVIVDANGYILTNNHVVEKADRIKVRLTG
                     DTMEYDAKLIGSDRETDMAVIKIEPGKKLTAAKIGNSDAVQVGDWAVAIGSPFGLEAT
                     VTAGIISAKDRAGTEQFQHFLQTDAAINPGNSGGPLLNINGELIGINTAIATQSGGYQ
                     GIGFALPINTAVKSYNQIIKTGRVTRGSIGVSLSVQPNPELLQVYGAKEGAFVQKVES
                     GSPADKAGLREGDVIVSMNGKPIRSGDDLVNGVADAEIGSEATLGLLRDGKKRDVKVT
                     IGDRTRVWARNPRISGEQAEESANDDSTEAKFGLFVRNLTEADREEFGFKDQGGVLVT
                     RVDQGSFADDVGLVRNDIIMSVNRRPVNSVDDIRKAQSGLKPGDAVAFHIKRARATLG
                     RRAGGDAEWTSLYLAGRLTAGQ"
     gene            9848..10063
                     /locus_tag="VLE22_19150"
     CDS             9848..10063
                     /locus_tag="VLE22_19150"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HSB16577.1"
                     /translation="MTFGRTLILIGAVIMAVGLVVSFGGRLPFRIGRLPGDILIKGKH
                     SVFYFPLATGLLLSLLLSLVLWLIHRR"
     gene            10133..10651
                     /locus_tag="VLE22_19155"
     CDS             10133..10651
                     /locus_tag="VLE22_19155"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HSB16578.1"
                     /translation="MILLFAAGLAVAAVQKKQQASSKQPASTRKGTTSKKTAAPKKST
                     APAKTVRRRTTAKTVTPQKTSKRARTRRIARRKSAPSWRRGQLEPTPERYREIQQALI
                     DRGYLQAPVPEKWGTESVDALKRFQQDQKLEASGKLDSLSIIALGLGPKRDLASQNGR
                     VPLAQPPPEGDR"
     gene            10648..11760
                     /gene="ald"
                     /locus_tag="VLE22_19160"
     CDS             10648..11760
                     /gene="ald"
                     /locus_tag="VLE22_19160"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HSB16579.1"
                     /translation="MIIGVPKEVKDHEARVGLVPSGAITLREAGHEVLVEAGAGRGST
                     ISDKEYEEAGACVVPTAAEVWSGADLVVKVKEPQLSEYGYLRKGLVLFTYLHLAPLPE
                     LTNRLLEAGVSAVAYETIREDDGSLPLLTPMSEVAGRMAVQVGAQYLEAPNGGRGVLL
                     GGIPGVAPANVLILGGGIVGHNAAKIAYGMGAHVTIIDKNLNRLREIDDIYNSQVVTL
                     ASNIWTIRETIRQADLVIGAVLIPGASAPRLVRRDMIPLMKRGAVMVDVSIDQGGCLE
                     TSRPTTHTDPVYLVDDVVHYCVSNMPAAVPHTSTFGLTNATFPYLLQLANNGLEKACE
                     DSAAIREGVNAYRGHVTHPAVAESQGRPFREFASLA"
     gene            complement(11793..13061)
                     /locus_tag="VLE22_19165"
     CDS             complement(11793..13061)
                     /locus_tag="VLE22_19165"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HSB16580.1"
                     /translation="MDDIQEQLAILRRKIRRIDRKYASPAPAPSPRPAEDRPFPDLPL
                     HYFIDEFLSGEVVETPHGSHFETEKLYARHRRHGSMDISNLEELPEDLLDSLSEGTVP
                     HTPPLRWAFLDTETTGLAGGTGTYAFLVGIGRITPEGFRVRQFFMRDLCEEASLLYAI
                     EQHLSQFDVLITYNGKAYDQPLLETRFRMARSRPPFGRLAHLDLLFGARRLWKLRLES
                     CRLVELEYQILGVEREGDIPGEMIPYVYFEYLRTRQAARLVPIFHHNAIDILTLACLT
                     GLVPLAFRSPEEAPLHHGADWVGLARWLLKAERHEEALALLRRAVERGLGDELLFRTL
                     WEIATVEKRLGREDAALAAFTELASIQNQFRIHALEELAKHYEHRERNYAMALEFTST
                     AIELSDTEELRRRAARLRRRLEKPRPVRLL"
     gene            complement(13162..15564)
                     /locus_tag="VLE22_19170"
     CDS             complement(13162..15564)
                     /locus_tag="VLE22_19170"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HSB16581.1"
                     /translation="METPNAMTAHGDAQAANAFSPELLGRMDAYWRAANYLSVGQIYL
                     YDNPLLKEPLRPEHIKPRLLGHWGTTPGQNFVYVHLNRVIREHDLNMIYISGPGHGGP
                     AVVSNVYLEGTYSEVYPLVTQDEAGLRMLFRQFSFPGGIPSHVAPETPGSIHEGGELG
                     YSLSHAFGAAFDNPSLVVACVVGDGEAETGPLATAWHSNKFLNPATDGAVLPILHLNG
                     YKIANPALLARIPRQELEQLMRGYGWEPYFVTGDDPALMHPLMASTLDRVIAQIQAIQ
                     REARELGIAACPRWPMIVLQSPKGWTGPKTVDGQPVEGTFRAHQVPLANTASNPEHLE
                     QLEAWLRSYRPEELFDTQGRFRPDLQALAPEGERRMGANPHANGGLLLKDLRMPDFTA
                     YAFDVPAPGAVRAEDTRVLGAFLRDVVRLNADRRNFRIWGPDETLSNRLGSVFEATSR
                     QWVAETKPADEFLAPEGRVMEMLSEHQCQGWLEGYLLTGRHGLFNSYEAFIHIVDSMF
                     NQHAKWLKVTLELPWRRKIASLNYLLASHVWRQDHNGFTHQDPGFIDHVVNKKAEIVR
                     IYLPPDANCLLSVMDHCLRSRHYVNVVVAGKHPAPQWLDMESAIRHCTAGIGIWKWAS
                     TDKDSDPDVVMACAGDVPTLETLAAVAILRRSLPELKIRVVNVVDLMKIQSPSEHPHG
                     LPDRDFDVLFTTNKPVIFAYHGYPWLIHRLTYRRTNHDNFHVRGYKEEGTITTPFDMA
                     VLNDLDRFHLAMDVIDRLPQLRACSAYLKQELRDKLVDHRRHIEKYGDDLPEVRDWKW
                     KS"
     gene            complement(15806..16141)
                     /locus_tag="VLE22_19175"
     CDS             complement(15806..16141)
                     /locus_tag="VLE22_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16582.1"
                     /translation="MPRKLKAELESAAAEAAWYASPEGHRQTQREFERALKRGTTLRS
                     KGSTIPLTHAKVLAELVEKAKANATRAISIRIPVADLDRAQQIAAKEGIGYQTVLKRA
                     IQAGLKKVS"
     gene            complement(16455..18380)
                     /locus_tag="VLE22_19180"
     CDS             complement(16455..18380)
                     /locus_tag="VLE22_19180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSB16583.1"
                     /translation="MIQLTAAGKRFGPKLLFEEADWQITAGDRIGLVGANGTGKTTLL
                     KILAGTETVDYGAASAMKGITCGFLPQYGLELSGRTVFAECLSVFEDLRLIERELESL
                     TRDMASLDPDGEAYRVVADRFHRLEDEFRNRDGYALEAQVGAVLSGLAFPKEDWTRRT
                     EEFSGGWQMRIALAKLLLERPNLLLLDEPTNHLDLEARNWLEQYLSSYPYAFVLVSHD
                     RYFLDVTVTRVVEIWNRRLNFYAGNYSRYLEQKAERRGQLEAAQKNQQDKIRQLEAFI
                     NRFRYTATKARQVQSRVKELERIERIELPPEEQTIHFSFPQPKPSGRIVAEFQGMSKS
                     YGAKRVFEDTGFVIERGERVALVGPNGAGKSTLIKILAGIESVSSGEYRLGHNVEPDY
                     FAQDQYKELDPSVRLLDDLGAIAPRSTLTELRTLLGCFLFSEEDVFKPVGVLSGGERN
                     RYALARMLLQPSNFLLLDEPTNHLDLRAKDVLLESLREFNGTVVFVSHDRYFIDRLAT
                     RVFDIGGGQVQSYPGNYEDFLWRKSSVAAAEAAEPAVVSDAPGNAAPAPDPVEEKPKR
                     LNPIRLRQMEERCTEIEEEIGRLETEITDHEQALAVFVSAEETRRISTLLDARRAELA
                     ALLAEWESLSRAIEETR"
     gene            complement(18441..19313)
                     /gene="accD"
                     /locus_tag="VLE22_19185"
     CDS             complement(18441..19313)
                     /gene="accD"
                     /locus_tag="VLE22_19185"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HSB16584.1"
                     /translation="MAWFTRQQPARQPGAPSEEEHRVKTEGLWVKCDNCRQIIWRKAL
                     DDNYQVCPKCSFHFKIDAAARLRMLFDEGKWEEFDRELVSTDPLEFVDQKAYRDRLAA
                     MRKATSLKDALISAVGLLNGRRVHICSLELKFIGGSMGAVMGEKITRAIERCIDQRMP
                     LVIVSASGGARMQEAAVSLMQMAKISAALMRLDESRIPFISVLTDPTTGGVTASFAML
                     GDINIGEPGALIGFAGPRVIEQTIRQKLPEGFQRSEFLVQHGFLDAVVPRTELKSYIA
                     RALEFFCGPVKNGD"
     gene            complement(19321..19854)
                     /gene="cobO"
                     /locus_tag="VLE22_19190"
     CDS             complement(19321..19854)
                     /gene="cobO"
                     /locus_tag="VLE22_19190"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSB16585.1"
                     /translation="MEERRGLLMVYTGNGKGKTSAAMGTAFRAVGQGFKVLMIQFIKG
                     SWHYGELDSAGMLGDAFEIRVMGRGFVKVGAEQPDPEDVRLVQEAWNAAVEAMQSKKY
                     DMLILDEINYAIHYGMLGIDQVLEGLVARPGQLHVVCTGRNAHPKLVEAADLVSEIKE
                     IKHPFHKGVLAQRGIEY"
     gene            complement(19864..20286)
                     /locus_tag="VLE22_19195"
     CDS             complement(19864..20286)
                     /locus_tag="VLE22_19195"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HSB16586.1"
                     /translation="MPRIWIYFIGKPRDHDANRLAAEFLKRTQRYAKCEMREVNPDRA
                     DLWKKHPGAAKILLDPTGKTMGSPAFAALIEKAEAEARDLVFVVGGADGLPAAWRPRA
                     DLLLSLSSMTFPHELARVMLAEQIYRAFTTLRGHPYPR"
BASE COUNT         3710 a         6118 c         6709 g         3851 t
ORIGIN      
        1 tctcatcctg attgctctcg ggtcgtcggt caccgtcggc acggtccgag ggcaggggat
       61 ctccttcctg cgggaattta ataactcgga ggctagggga gtggccgtgg atgccagcgg
      121 catctacgta gcgggcggca cgggtatgag tggtttcgta cgcaagttcg acctcagcgg
      181 caaccacctg tgggcccgtg tatccgggtt gggcgctgcc gcaatcgtgg caagtaccgg
      241 cggagtgtac gtcgtaggaa gcgacgccgc agccggcttc attcgcaagt acgatgcgga
      301 aggcaacgag ttgtggtccc ggcagacgca caactccatc gcgagtgcgg tggcggcaga
      361 tgccacgggc gtctacgtgg gcgactacga tcccgccccg cgtaaggacg gctctgtgcg
      421 gaagtacact gccgacggcg aggaactgtg gagccatcag ctcaggggag gcccgtggcc
      481 tgtcggcgtg gccgtggatg ctagtggcgt ctacgtggtc gtccgcgact ccgtgaagaa
      541 gtacactgcc gacggcaaag aactgtggac ccggatgttc tggcaaggag gcgcctggcc
      601 cgtcgccgtg gcagtggaaa cgacgggcat ttatgtggtc ggcaacacag atacataccc
      661 aaatccagaa cagattgtct tagtcaagta tgactcggaa ggaaacgagc tttggatccg
      721 ccaatttgac tggttggacg ttgggggttt cgttgcgccg agcggcgtgg cggcggacgc
      781 gaccggcgtg tacatcgtgg ggcagacaac gcgccgattg cccgaccagt gcgaatccag
      841 cggccaggac gtgtttgtac gcaagtatgg gcccaatggc gtgtcccttt ggagccgccg
      901 gtttttgggc tcgggggaca taggttacgc gtccggtgtg accgtggata ccacgggggt
      961 ctatgtcgtc ggaagttcag tttctaatac ctgggacggt ctgcgcaaca gttcgcgcac
     1021 caatgccctg ctggccaagc tcgagaaaac gcagccggtg atcggcgaat cggggccctg
     1081 gatctggccg ggctgcgtcg tcaacgctgc cagctacctg ggtggacgaa tttcccccgg
     1141 tgagatcgtg acggttttcg gacgccggat cgggccgcca gaggcgactc ctccgcttca
     1201 tctcacggca ggccgccggc tggcaaccac gctggccggc acccgcctcc tgttcaacgg
     1261 catcgcagcg ccgctctact tcgcgtcggc caaccagatc agtgccatcg ttccatatgc
     1321 agtggccgac aaacccacgg cggacgttga ggtcgaatac cagggggtgc gctcgaacaa
     1381 agtaacgatt ccggtggcag cttctcgtct gggcgtcttc acctcggacc ggtccggcag
     1441 cggacctgcc gcagccttga acgaagatgg gagcattaat tcgagttcga atccagcaaa
     1501 acaaggctcc atcattgttc tgtatgcgac cggcgaaggg ctgactgagc cggtcgtcga
     1561 ggatggcgtg gttctcggtt cggtactgcc gaagccgaag ctgccggtct cagtctcatt
     1621 tcttgctcct ggcaaacctt gtgatcgaca tgattactac tcttgctacg acagttactt
     1681 tgtcaccggc agggttctat acgccggagg ggcgcccagg tcggtggccg ggctgcttca
     1741 agtgaatgtg cggctacctc cggatgctta ccccgatgtt gggtataccc aggtggaatt
     1801 catcctctgt gtaggcgacc ggttcgtgag tgggtcgttg tacattaggc gttgaggcgt
     1861 gctcaaggca gactcggatg cgttcgcgag agtggaagaa gccgtgagcc gcaagccgca
     1921 catcgccggc atactcgccg ggctgaccct ggcctggtgc cggagtgact cgaccgctcc
     1981 tccgtcatcc gccgactttg cactttaccg ctttgacgac aacctgaaga cagccacggc
     2041 gaaggccccg cagcgtccct catcgccgcg gctggccgag ccggtagttt ggaagaactt
     2101 ctacaccgag aaggcctcca cctggatccc ccggggcggc agcatgcggc tgcgcaacgg
     2161 agacctgctt ctgcaaggtg acggcggcct gcccgtgatc ctttcgccga aggattttcc
     2221 gatcgactgg agcttgtatg aggcggtgcg catccggatg atggccgaag ccggcgaaga
     2281 agtgaggatc aagatcgggc ctgacgagtt cacgcagaag ctcggcccgc ccggccagta
     2341 tcaggtttat cggttcgacg tgaacatcca tccccccaag ggcagccgcc cgctggctat
     2401 cgttccgacc gacagcctca ccgggctggt ggcagtcgat ttcatcgagc tagtgccgcg
     2461 ccgagcatac tttcatgagg ctgtcggacg tcagttcctt ggcaaacgtg atgactacgt
     2521 gaacgcgctc tacgcccact ccccaagctc cttgacgtac aacgtgcgcg tgccggagca
     2581 cgccaagttg cgcttccgca ttggcgtggc ggagagcaaa gcagtagcgt tcagggtagc
     2641 cgccgaaggc accgaactgt tccgccgcac acttgaggat ccctcagcgt gggaggatgg
     2701 cgaggccgat ctaagccggt tcgcaggccg gacgatcaaa ctggttttcg agacgagttc
     2761 tgcccaggaa ggcgcggtgg ctatgtgggc caaccctcgg atgacggcga ccggggcgcg
     2821 gagccgtccc aacattctgc tgtatctggt ctgcacgatg cggcgggatc atatgagcct
     2881 gtacggatac ccacgcgaca cgacgccgtt tttgaagaca ctgggctcgg cgggggtggt
     2941 tttcgaggac gcgcatgcgc aggcagcgtg gaccaaggcc tcggtgcctt cgctgatgac
     3001 ctcgctctct gcttacgcca cgggcatcgt gcgggagaat gacacgatac cgtcgggcgc
     3061 cacaacgttg gccgagcagt tgaggcgtgc gggctacgtg acggcgagct tcgtgacgag
     3121 tccctttgcg ggcaaggcaa gcgggctgga ccgcggcttc gattacgtgg cggaacatcg
     3181 gctcattcag cggatgcgca gtgaagaaac ggaccgcggt acggactcgg cagcgctcaa
     3241 caaggtgctg gcggcgtggc ttgagcgata ctgggacgaa ccgttctttc tttacgcaca
     3301 ctcaaacgat ccccacgcgc catacgatcc cccggcggag ttcgaggcga aattcgcgaa
     3361 cccggccgag acggcacaat tcaaccgcga ctacgggctc ctgcgggaca ggcacatgta
     3421 cggcggagga gctaccatca gccgagccga ctgcctgcgg aagggaattc atccggacag
     3481 gttcctcagg cgggcgatcg agcgttatga cgccgagatc gcccacaacg actcgagcat
     3541 cgagcggctc gcgggcaaac tgaaagagct cggaattctc gacaatacgc tgatcatcgt
     3601 cgtctccgat cacggcgagg agttctggga gcacggctgg acagggcacg gccactcgct
     3661 gtatcaggaa ctcacccaag tggcgtggtt gatgtggaat ccgaagatgt tgccttcacc
     3721 gaagcggatc ggcgagccgg tgcaactgat ggatctcgtc cctaccgtgc ttgatctgct
     3781 gaagattcgc ccctcgatca cgctgaaagg acagagcctc ctgccgctcc tccgtgggaa
     3841 gacatttgag cggaagacgc cggtgatggc atcacgtttc gcgcatccag gggccaagcc
     3901 gcatggtttt gtgccggaga accgcacggg cacgttcgct ctgatcacgc gggactggaa
     3961 gctgatctgg cgggatcaag ccaggctctc cgcgttgccg gagatcgaac tgtacgaccg
     4021 ccgcacggac caggccgaga ctcgcaacgt gtcggcgcag tatccggaag tggttcggcg
     4081 gctgaaagcg gagatggacc agtgggtcga ggcccagaaa ccggtgcgca agctggtagg
     4141 cgccactggg cacacctccc tgagtcccga ggcgatcgag cgtctgcgga gtttgggcta
     4201 tatcgggaag cagtgacgtt ttggaattcg cacgagaccc actggggggc aggagggaag
     4261 caggaggata ttcctgtcag tgagcaaagg agggaccatc acgacggctt ccgcagtaca
     4321 ccgagggatt gacggtccaa gagtcgcctt tgtcagtgag aacatccgcg cggtcttcga
     4381 cgcttgcgag gtagcatacc atcgctggct cggcccaact tccgggtgac ctgttcgccc
     4441 aagtctcgcc caaattaaac agccactcaa tccgaaggtg ttttggcggt ccacgtgccc
     4501 cggctgaaac cgatttcaga aagcggctgc agaccagcct ctcgttcgaa ccgggcaccg
     4561 tcgagacccg ggtgccccca ctagcaatcg gttaacagtg ccccgcaggc cttcatgccg
     4621 ccaccgtccc taatgctatc gcgcacagag gataatgtct gggcgcatga cgacccccga
     4681 cacccagcac cgccgcttcg cctggcttat catcctcctg ctcttcgccg gctcggtgct
     4741 caactacgtc gaccgcgccg tgctcggcgt gctcatgccg caaatccgcc acgacctctc
     4801 gctctcctac tccgactaca gcctcgccgt gaacgccttc ctcgtcatgt acacgatcct
     4861 ttacgtgctc ggcgggcgca tggcggacca gctcggctat cgccgcagct tcaccctgac
     4921 gcttgccttc tggtcgttcg cctgcatgct tcacgcggtc acgcgcggac tggccagcct
     4981 ctgcctgttc cgcggtctgc tcggcatcgg cgaaggcggc tattatcccg cggcgatgcg
     5041 cggagtggcc gattggttcc cgccacagaa ccgcgccaag gctgtgggag tgcttctgtg
     5101 cgggatcagc ataggcacgc tgctcactcc gcccttcgtg gcctggctga cggcaatcta
     5161 cggctggcgc tcggcgttcc tcgtgatcgg agcgttcggc tttttgctga tcccgccatg
     5221 gctcgcgctg caccgccgga tcgcggccgc ctacggcacg tccggcccgg cgcacgcagc
     5281 acagccggcc gcgtcagcct ctgacggcga cgacccttcg atcgccgaag tgctcacccg
     5341 ccgcaaatac tggtgcatcc tgagcgcgcg ggcgttgacg gattcagctt ggtatttcta
     5401 cctcttctgg atgcccgggt actttcagga ggtgcgccac ttcgacttgc ggatggtcgg
     5461 ggcgctgctg tggatcccct acttcggggc gaacctcggc gcattaggcg gcgcctgggc
     5521 gtcgagcggg ctgatcgcgc gcggctggag cgttgaccgc gcccgcaaga gcctcctgat
     5581 cccgtccgct gcggtctgca cgctcggagc gctgaccggc ttcgtcccga gcagctacgt
     5641 agcgctcgcg ctcgtgagcc tggcgctgct cggccaccag tcgtggtctt cgaacatcca
     5701 caccgtgatc acggagatca cgccgccgaa gcacgtcgcc atgctgtacg ggatcaccgg
     5761 cgccgccggc acgttgatgg gcgcgctcac gcagcccgcc gtcggccgcg tggtggacgc
     5821 cctcggctac gagcccgcct ttgtcgccgc cggctcggcc tacgccgccg ccatcgtcct
     5881 gctgatcgcc gccggacgca tcgagcgcat ccgccgcggg cggcccgctg cgtagaggcg
     5941 gtacactgtc tccgatggcg cggctgctcg atcagaaggc aacgcgggcg atcgacgagc
     6001 tggtgaaggc tcaggcagta caggactgtc tccagttttt cgccggcgag aaacagtgga
     6061 tcaacgagcg ccacatcgag ctttgccgca ttcccgcgcc gacctttctc gaggagcggc
     6121 gcgcggagtg gatggccgcg cagttcgctg cgctgggctg gcacgtcgaa gccgatggcg
     6181 ccggcaacgt aatcgcccgc cccgacgcgg cgcgaaccgg gccttacatc gcgctcacgg
     6241 cccacctgga caccgtgctc tctccgcgca accccgagga catcaaggtc gagccggacg
     6301 ggaagttcac cggaccgggc gtctccgaca acggtgcggg actcgcggcg cttctcgccg
     6361 tcgcccgagc ctggaagttg tcgtgccccg taccggattc cgaggtgcgc ctacttctgg
     6421 tggcgaacgt gggcgaggaa ggcgagggca acctcagcgg aatgcgctac ttgtgcaaca
     6481 agcggccgct cgccgacctc atccagacgt tcgtggtgct ggacggaccg gcaaccgacc
     6541 acatcacctg ccaagctctc gaaagccgcc gcttcgaggt gtcgttcgcg ggccccggcg
     6601 ggcacagctg gagcgaccac ggcatcggaa acccagttca tgcgctcggc cgggccatcg
     6661 cgttcttctg tgacttgcag acgaacggtg gaccgtccgg gcacgcgcgg acgtcgttca
     6721 atttcgggat catcgacggg ggggcgagcg ttaactcgat cccgacttcg gcgcgtgcga
     6781 agctcgatat ccggtccgaa aacggcgcaa gaatggacga aatgtgcacg tttttgagca
     6841 cttccgtgca aaaagcgctc gaagttgaga acgaccgcgc cagcaaaggc cgcgtttcgg
     6901 cccgcatccg ggagatcggc gcgcgtcccg gcggcgtcct tccggaagac tcgccgatcc
     6961 tttcttacgt tcgggcggcg gatgcatggc tcggtctccg cgctcgtctc gactgtgcct
     7021 caacagacgc caacatccct ctttccatga gcttaccggc catctctatc ggggctggcg
     7081 ggcaaggcgg cggcgcgcac acgcccggcg agtggtacca gcccgaaggc cgcgacctgg
     7141 gcctccagcg catcctcctc actctgtgcc tgctgatgcg cgaaccggcg gtgactggcg
     7201 gatgatttcg cctgttgttc gcccgcgctg gggcgccgac ctggctctgg cggggatcgc
     7261 cctgatctgg ggtgcgacgt tcgtgctggt gaagcaggcg ctggccgacg tctccacaat
     7321 cctgtttctc gcgctgcggt tcctgcttgc ggcggcggct ttggcgctgg cactgcgggg
     7381 gcggtacgcc ggatttttgg agcgcccgaa ggcgctggca gggggcgtgc tggcggggct
     7441 cttccttttt tccgggtatg cattccagac ggcagggctg cgctacacga cgccgtccaa
     7501 atcggcattt atcaccgggc tgtcgatcgt gctggttcct ttggtcactt cgctcgtcta
     7561 tagaactgcg cctcgcattc tcgaaggact tggcgtgatg gtggcggcgg ccggcctggc
     7621 gctcatgacg ctggaagatt tgagcgttgg gttaggctac ggcgatttat tgacagtgtg
     7681 ctgcgcggtc gctttcgcag tacacatcgt cgcggtgggc cactgttcgc ggcgcttcgg
     7741 attcgagggg ctgagcgtgg tgcagatcgc cagcgccgcg gtcttttcgc tggcgacttt
     7801 ctggtggatg gaaacgccgc aggtgcgatg gacggccggc ttgctggtcg cgctggcggt
     7861 gacgggtctg ctggcgacgg cgctcgcctt caccgtgcag gcgtgggcgc agaagcacac
     7921 cacggcgacg cggacggcgc tgatcttcgc gctggagccg gtttttgcgt gggccacttc
     7981 atacattgtg gcgggcgaga cgctgtccaa ccgagcggtg acgggagcga ttttgatttt
     8041 ggcaggcatc gtgctagtcg aactgaaacc actgtccggt ggggagcatc cataaagaca
     8101 gaacctgcgc aatctaagat gctctagaat tgaaggtaag agcgccttcg ggagacaaga
     8161 aagactatga gtttgttcac gacgatacgc aggcagaagc tgctgtccct cagccttctg
     8221 ctgttcaccc tgtccctagg aattctgatc ggcacgttgg tgaattccgg tgtcagggcc
     8281 gagaggggac agacggcggt cgatgccacc ccgctcacga ttcccaatcc cgtacagttg
     8341 tccaccgcgt tcaaccaact cgcgaagaaa gtggaaccaa cggtcgtcaa catcagggcc
     8401 gactatctcg cgcgtgacac ccagtcatcg cggaatcgcc ggggccagaa tccgcagggc
     8461 gaggaaggcg atccagggtt cgatttcttc caccggttct tcggcggccc cttcgggcag
     8521 gcgcctgaaa tgccgttccg ccgggaggga accggatccg gtgtgatcgt tgacgcgaat
     8581 gggtacattc tcacgaacaa ccacgtggtc gagaaggccg accggatcaa ggtgcggctc
     8641 acgggcgaca cgatggagta cgacgcgaag ctgatcggtt ccgaccgaga gacggacatg
     8701 gcggtcatca agatcgagcc gggcaagaag ctgactgcgg cgaagatcgg caactcggat
     8761 gcggtccagg tgggagactg ggcggtagcg atcggttctc ccttcgggct ggaggccacg
     8821 gtgacggcgg gaatcatcag cgcgaaagac cgggccggca cggagcagtt ccaacatttc
     8881 cttcagaccg acgccgcgat caatccgggc aacagcggcg ggccgttgct gaacattaac
     8941 ggcgagctga tcgggatcaa cacggccatt gccacacaga gcggcgggta ccaaggcatc
     9001 ggtttcgcgt tgccgatcaa caccgcggtg aagtcgtaca accagatcat caagacgggc
     9061 cgcgtcacgc gcggctccat cggggtaagc ctgagtgtgc agccgaatcc cgaactgctg
     9121 caggtttacg gcgccaaaga gggcgcgttt gtccagaaag tggagtccgg cagcccggcc
     9181 gacaaggcgg gtttgcggga gggcgacgtg atcgtcagca tgaacggcaa gccgatccgc
     9241 agcggcgacg accttgtgaa cggggtagcc gatgcggaaa ttgggagtga agccactctt
     9301 ggccttctcc gcgacggaaa gaagcgcgat gtgaaagtga cgatcggcga tcgcacccga
     9361 gtgtgggcca ggaatccgcg gatttcgggc gagcaggccg aggaatccgc caacgatgat
     9421 tcgaccgaag cgaagttcgg gctgttcgtg cggaacctga ccgaagccga ccgggaagaa
     9481 ttcgggttca aggatcaggg gggcgtgctg gtcacgcggg tcgatcaagg ctcgttcgcc
     9541 gatgacgtgg ggctggtgcg aaacgacatc attatgtcag tcaaccgccg gccggtgaac
     9601 tcggtggacg acatccggaa ggctcagagc ggcttgaagc caggagacgc ggtggcgttc
     9661 cacatcaagc gagcgagagc aacactcgga cgccgcgctg gcggcgacgc ggagtggacc
     9721 agcctgtacc tggcgggccg gctgacggct ggacagtaac agagccctct tttcgcaggc
     9781 gttgtgaacg ggcgaggctc gcacatcgag agcctcgccc gttttgctga gacgtgagta
     9841 atgggagatg accttcgggc gaacgctgat tctcattggg gctgtcatca tggcggtcgg
     9901 gctggtcgtc agctttggcg ggcgcctgcc gttccgcatc ggccgcctgc cgggcgacat
     9961 tctgatcaaa ggcaaacatt ccgtgttcta cttcccgctg gcgacgggcc tgctgttgag
    10021 cctgctgctg agcctcgtgc tgtggctgat ccaccggcgg taggagatca gagctggccg
    10081 tttttcagtt ttgttacact gtgacttgcg tttacgcaga gcagcttttc tcttgatttt
    10141 gctgttcgcg gccggacttg cagtggcggc cgttcaaaag aaacagcaag cgtcctcgaa
    10201 gcagccagct tcgacgagga agggcaccac ctccaagaag accgcggcgc cgaagaaaag
    10261 caccgcaccg gcgaagacgg tacggcggcg cacgaccgcg aaaaccgtca ccccccaaaa
    10321 gacgtcgaag cgtgccagga cacgccggat cgcgcgaaga aagagcgcac caagctggcg
    10381 tcgcggccaa ctggagccga cgcccgagcg gtatcgggaa atccaacagg cgctcatcga
    10441 ccggggttac ctgcaagctc ccgtgccgga gaaatggggt acggagagcg tagacgcgct
    10501 gaagcggttc caacaggacc agaagctcga ggccagcggc aagctagact cgctgtcgat
    10561 catcgccctc ggcctcggac cgaagcggga cctggcatct caaaacgggc gggtacccct
    10621 cgcccagcca ccaccagagg gagatcgatg atcatcggcg ttcccaagga agtgaaagac
    10681 catgaggccc gggttgggct cgtgcccagc ggagcgatca cgctgcgcga ggcgggccac
    10741 gaagtgcttg ttgaagccgg agccggccgc ggcagcacga tttccgacaa ggaatacgag
    10801 gaggccggcg cgtgcgtagt accgacggcc gctgaagtct ggtcaggcgc ggacctggtg
    10861 gtgaaggtca aggaaccgca gttgtccgag tatggctacc ttcgcaaggg actggtcctg
    10921 ttcacctatc tgcatctcgc accgctgccg gagctgacca accggttgct tgaggcgggt
    10981 gtcagcgcgg tggcatacga gacgatccgg gaagacgacg gctcgcttcc gctgctgacg
    11041 ccgatgagcg aggtggcggg ccgcatggcg gtgcaggtgg gagcgcagta cctggaagcg
    11101 ccgaacggcg ggcggggcgt gctgctgggc ggaattcccg gcgtcgcgcc agccaacgtg
    11161 ctgattctgg gcggcgggat cgtggggcac aacgccgcga agatcgcgta cggcatgggc
    11221 gcacacgtca cgatcatcga caagaacctg aatcggctgc gggagatcga cgatatctac
    11281 aacagccagg tagtgacgct ggcgtccaat atctggacga tccgcgagac gatccggcag
    11341 gcggacctgg tgatcggagc ggtcctgatt ccgggcgcgt cggcgccaag gctggtgcgg
    11401 cgggacatga tcccgctgat gaagcgcggc gcggtgatgg tggacgtctc gatcgaccag
    11461 gggggctgcc tggagacgtc gcggccgacg acgcataccg acccggtcta tctcgtcgac
    11521 gacgtggtgc actattgcgt ctcgaacatg ccggcggcgg tgccgcatac atccacgttc
    11581 gggctgacca acgccacgtt cccctatctg ttgcagcttg ccaacaacgg gctcgagaag
    11641 gcctgcgaag attccgctgc gattcgcgag ggtgtgaacg cgtaccgcgg gcacgtcacg
    11701 cacccggcgg ttgccgaatc ccaagggcgt ccgttccggg agtttgcctc gctcgcataa
    11761 gcgcccgccc ggcggattcc gcggggtcga gtttagagga ggcggacggg gcggggcttt
    11821 tccaggcggc ggcgcaggcg ggcggcgcgg cggcgaagtt cctccgtatc acttaactcg
    11881 atcgccgtgg aagtgaactc gagtgccatg gcgtagttcc tctcgcggtg ttcgtagtgc
    11941 ttggccaact cttcgagggc gtggatccgg aactggttct gtatggaagc aagctccgtg
    12001 aacgcggcca gggcggcgtc ctcacggccg agacgttttt cgactgtcgc gatctcccaa
    12061 agtgtgcgga aaagcagctc gtcgcccagc ccgcgttcga cagcgcgtct gagcagggca
    12121 agcgcctctt catgacgctc ggccttgagc agccagcgcg ccagtccgac ccagtcggcg
    12181 ccatggtgaa ggggcgcctc ttcgggcgac cggaaggcga gcggtacgag gcccgtgagg
    12241 caggcgaggg tcaggatgtc gatggcgttg tggtggaaga tgggaacgag acgggcggcc
    12301 tggcgtgtgc gaaggtactc gaaatacacg taggggatca tttctcccgg gatgtcgcct
    12361 tcgcgctcga cgccgaggat ctggtattcc agttcgacga ggcggcagct ttccaggcgc
    12421 agtttccaaa gacgacgcgc gccgaagagc agatcgagat gggcaaggcg gccgaacgga
    12481 ggccgggagc gcgccatgcg gaaccgcgtc tcaaggagcg gctggtcgta ggctttgccg
    12541 ttgtaagtga tcagtacgtc gaactgcgac aggtgctgct cgatcgcgta gagaaggctg
    12601 gcttcctcgc aaaggtcccg cataaagaac tggcggacgc gaaacccctc gggggtgatg
    12661 cggccgatgc cgacgaggaa ggcgtacgtt ccggtgccgc ccgcgaggcc ggtggtttcg
    12721 gtgtcgagga aggcccaacg caatggcggc gtgtgaggca ccgtgccctc ggacagtgaa
    12781 tccagaagat cctcgggcag ctcctcgagg ttcgagatgt ccatgctgcc gtggcggcgg
    12841 tggcgggcgt agagcttttc ggtctcgaag tggctgccgt gcggggtttc gacgacctcg
    12901 ccgctcaaaa actcgtcgat gaagtagtga agcgggaggt cggggaaggg ccggtcttcg
    12961 gccggccgcg gagacggggc aggggcgggc gaggcgtact tccggtcgat gcgccggatc
    13021 ttgcgccgga ggatggcgag ttgttcctga atatcgtcca cgccctattc gccttttctt
    13081 tgcccactat agaacggcgg agacgcggag ttcaagacac aagagatgtc gaagggtgtc
    13141 gttgtggcct cgcccgcgcg gtcacgattt ccatttccag tcgcggactt ccggcagatc
    13201 gtccccgtac ttctcgatat gccgccggtg gtcgacgagt ttgtcccgca attcctgctt
    13261 cagataggcg ctgcaagcac gtagctgcgg caatcggtcg atgacgtcca tagccaggtg
    13321 gaagcggtcc aggtcgttga ggacagccat atcgaagggc gtcgtaatcg tcccctcttc
    13381 cttatagccg cggacgtgga aattgtcgtg gttggtccgg cgatatgtca ggcggtgaat
    13441 cagccagggg taaccgtggt acgcgaaaat caccggcttg ttggtggtga acagcacatc
    13501 gaagtccctg tccggcaggc cgtgcggatg ttcactcggg gactggatct tcattagatc
    13561 cacgacgttc acgacccgga tcttcagctc cggcaggctg cgccgcagga tcgcaaccgc
    13621 ggcgagggtt tcgagtgtgg gcacgtcgcc cgcacacgcc atcaccacgt cgggatcgct
    13681 gtccttgtcg gtgcttgccc atttccagat gccaataccc gccgtgcagt ggcgtatagc
    13741 cgattccatg tccagccatt gcggagcggg atgcttgccg gctacgacga cgtttacata
    13801 atgccggctg cgcaggcaat ggtccatcac ggaaagcaag cagttcgcat cgggcggcaa
    13861 gtaaatgcgt acgatctctg ctttcttgtt caccacgtgg tcgatgaagc cggggtcctg
    13921 gtgagtaaag ccgttgtgat cctgccgcca aacatggctc gcgagcagat agttcagcga
    13981 agcgatctta cggcgccatg gcagttcgag cgtaactttc agccacttcg cgtgctgatt
    14041 gaacatcgag tccacaatgt gaatgaaggc ctcgtagctg ttgaacaggc cgtggcggcc
    14101 ggtcaggagg taaccttcca gccaaccctg gcattggtgt tcgctcagca tttccatcac
    14161 ccgcccctcc ggcgcgagga actcgtcggc cggtttcgtt tcggcaaccc attggcggct
    14221 ggttgcttcg aatacggaac ctagccggtt ggagagagtt tcatccgggc cccaaatccg
    14281 gaagttccgc cgatctgcgt tcaaccggac gacatcacgg aggaatgcgc caagcacacg
    14341 ggtgtcttcg gcccgcacgg ctcccggcgc ggggacgtcg aaggcgtacg cggtgaagtc
    14401 aggcatccgt aaatccttaa gcagcagacc gccgttggcg tgcggattcg cacccatgcg
    14461 ccgttcgccc tcgggcgcca gcgcctgcag atccggccgg aaacggcctt gggtatcgaa
    14521 cagctcctcg ggacggtagc tgcgcagcca agcctcgagt tgttcgaggt gctcggggtt
    14581 gctggccgtg ttggccagcg gcacctgatg ggcgcggaag gtcccctcca cgggctgccc
    14641 gtccactgtc ttcgggccgg tccagccttt gggggactga agcacgatca tgggccagcg
    14701 cgggcaagcg gcgattccga gctcccgcgc ttcccgttga atcgcctgga tctgcgcgat
    14761 gacgcgatcg agcgtggacg ccatgagcgg gtgcatgagc gctggatcgt cgccggtcac
    14821 gaagtacggc tcccaaccgt agccgcgcat cagttgttcg agttcctgcc gcggaatgcg
    14881 agcaagtagc gctggattgg cgatcttgta cccgttcagg tgcaggatgg gcagcacggc
    14941 gccgtcagtc gccggattca ggaacttatt cgaatgccag gcggtggcga gcggcccggt
    15001 ttcggcctcg ccatcgccga ccacgcacgc aacgacaagg ctcgggttgt caaacgccgc
    15061 tccgaatgca tggctgagcg aataccccag ttcgccgcct tcatgaatgg agccgggcgt
    15121 ctccggcgct acatgactcg gaatgccgcc ggggaaggaa aactgccgga acagcatgcg
    15181 aagccctgcc tcatcttgcg tcaccagggg gtagacctcg ctgtacgtcc cttccaaata
    15241 cacgttggag accaccgctg gtccgccgtg tcctgggccg gagatgtaaa tcatgttgag
    15301 gtcgtgttcg cggatgacgc ggttcaagtg aacgtagacg aagttctgcc cgggcgttgt
    15361 gccccaatgg ccaagaaggc gcggcttgat atgctcaggc cgtagcggct ccttaagcag
    15421 cgggttgtcg tacaggtaga tctgtccgac ggacaggtag ttagctgccc gccaataggc
    15481 gtccattcga cctaggagtt ccggtgaaaa ggcgttcgcc gcctgggcgt cgccgtgtgc
    15541 tgtcatcgca ttgggggttt ccataatgcc tcgttccgcc ttcaggaggg tgccaacaaa
    15601 cccgccggca ttgacgcctg acacagccta aaggctgtgc cacgaccgag gatcaagagc
    15661 ttactgcctg tagtggcaca ggcctcaacc tagcttcagc ctgtagcatg ggtcttttca
    15721 gcaccccgtg tttccgcccg cattcaggct agcatatgag actgctggga tcggacctga
    15781 cggttgtgat tcggacgtga gaagcttacg agaccttctt cagtccggct tggatcgctc
    15841 gcttcaggac ggtttggtat ccgattcctt ccttggctgc gatctgttga gcccgatcga
    15901 ggtccgcaac gggtatccgg atggaaatgg ccctcgtcgc gtttgccttg gccttctcca
    15961 ccaactcggc aagtactttt gcgtgggtca acgggatggt cgagcctttc gagcgcaggg
    16021 tggtgcctcg tttgagagca cgctcgaatt cccgttgcgt ttgccggtgc ccctccgggc
    16081 tggcatacca agcagcttct gccgccgcgg actccaactc agccttcaat ttccggggca
    16141 taaaccctcc tttcggcttg attcatggtg tacgccgtca ccgtccgaaa cctcttgcgc
    16201 cggacggtga acacgaccac gagataacgt ccgcctgccg tccgtccgaa cagtttccaa
    16261 cgcttttcgc ttcctaccgg cgcagccgga atgatgacgc ggcggcgtct tgccgtctcc
    16321 tcgacttctt caggtgacac gccgtggagg gcggtgtggc caacattgtg tacatcccac
    16381 tcgaagccga gaagtatata caggtagcgt atatacgcgc aagatccgca ttccgtacgc
    16441 ccccgcccgg atactcagcg agtttcttca attgcgcggg agagggattc ccattcggcg
    16501 aggagggcgg cgagctcggc gcggcgagcg tcgaggagcg ttgagatgcg gcgagtctct
    16561 tcggcgctca cgaagaccgc tagcgcctgc tcgtggtcgg tgatctcggt ctcgaggcgg
    16621 ccgatctcct cttcgatctc agtgcagcgt tcctccatct gtcgcagccg gatcgggttc
    16681 aggcgcttcg gcttctcctc gacggggtcg ggtgccggag cggcgttgcc gggcgcgtcg
    16741 gagacaaccg cgggctctgc cgcctcggcg gctgccaccg atgatttccg ccagaggaag
    16801 tcttcgtagt tgccgggata cgactgtacc tggccgccac cgatatcaaa cacgcgggtc
    16861 gccagccggt cgatgaagta ccggtcgtgc gaaacgaaga ccacggtgcc gttgaattcc
    16921 cggagggatt ccagaagaac gtccttggcg cgcagatcga ggtggttggt cggctcatcg
    16981 agtagcagga agttcgacgg ttgaagcagc atgcgcgcca gggcgtaccg gtttcgctcc
    17041 ccgccggaca gcacgccaac gggtttgaaa acgtcctcct cggagaataa gaaacaccca
    17101 agcagcgtgc gaagctccgt gagggtcgat cggggggcga tcgcacccag atcgtcgagc
    17161 agccgcacgg aggggtccag ttccttgtat tggtcctggg cgaagtagtc gggctcgaca
    17221 ttatggccga ggcgatattc tcccgacgag acggattcga ttccggccaa gatcttgatc
    17281 aacgtcgatt ttcccgcgcc gttaggcccg accagtgcga cgcgctctcc gcgttcgatg
    17341 acaaagccgg tgtcttcgaa cacgcgcttg gcgccatacg acttgctcat gccctggaat
    17401 tcggcgacga tgcggccgct tggcttcggc tggggaaagg agaagtggat tgtttgctcc
    17461 tcggggggca actcgattct ctcgatccgc tccagttcct tcacgcggct ctggacctgg
    17521 cgcgccttag tggccgtata gcggaagcga ttgatgaacg cctcgagctg gcggatcttg
    17581 tcctgctggt tcttctgtgc cgcttcaagc tggccgcggc gctcggcctt ctgctcgaga
    17641 tagcgcgagt agttgcccgc gtagaaattg agccgccggt tccagatctc gaccacacgg
    17701 gtgacggtga catccagaaa atagcgatcg tgcgagacaa ggacgaaggc gtaggggtag
    17761 gaggagaggt actgttccag ccagttcctc gcctcgaggt caaggtggtt ggtcggctcg
    17821 tcgagcagca gcagattcgg gcgctcgagc agcagcttgg caagggcgat ccgcatctgc
    17881 cagccgccgg agaactcctc ggtgcggcgg gtccagtctt ccttcgggaa ggccagaccg
    17941 ctcagaacgg cgcccacttg cgcctcgagc gcgtagccgt cgcggttacg gaactcgtct
    18001 tcaagccggt ggaagcggtc ggcgacgacg cggtacgcct cgccgtccgg atcgagcgag
    18061 gccatgtctc gggtaagcga ctcgagttcc cgctcgatga gacggaggtc ctcaaaaacc
    18121 gagagacact cggcgaagac ggtgcggccg gaaagctcga ggccgtactg cggaagaaag
    18181 ccacacgtaa tgcccttcat cgccgaggcc gcgccgtagt cgacggtctc ggtcccggcg
    18241 aggattttca acagggtcgt cttgccggta ccgttggctc ctaccaggcc gatgcgatcg
    18301 ccggcggtga tctgccagtc cgcctcttcg aagaggagtt tcgggccgaa gcgcttgccg
    18361 gcggcggtga gctggatcat tgagagaggg tgaggaaaaa tggtgacaga cagccatttc
    18421 gcaaaggcgg cgaaatggag tcagtcccca tttttcacgg gtccgcagaa gaattcgagg
    18481 gcacgggcga tgtagctctt caactcggtt cgggggacga cggcatcgag gaagccatgc
    18541 tggaccagga actcgctgcg ctggaagccc tcgggcagtt tctggcggat ggtctgctcg
    18601 atgacgcggg ggccggcgaa gccgatcagc gctccgggtt cgccgatatt gatatcaccg
    18661 agcatggcga agctggcagt gacaccgccg gtggttgggt cggtcagcac gctgatgaag
    18721 gggattctcg actcgtcgag gcgcatgagc gcggccgaga tcttggccat ctgcatgagg
    18781 ctgaccgcgg cctcctgcat gcgcgcgcca ccggaagcgg agacgatgac gagcggcatt
    18841 cgctggtcga tacagcgctc gatggcccgg gtgatctttt cgcccatgac ggcgcccatg
    18901 ctgccgccga tgaacttcaa ctccagagaa cagatatgca ctcggcggcc gttgagcaag
    18961 ccgacggcgg agatgagcgc gtccttgagc gaggtggcct ttcgcatggc cgccagacgg
    19021 tcgcggtagg ccttctgatc cacgaactcg agcgggtcgg ttgagacgag ttcgcggtcg
    19081 aactcctccc atttgccttc gtcgaagagc atgcggaggc gggcggcggc atcgatcttg
    19141 aagtggaagc tgcacttcgg gcagacctgg tagttgtcgt caagcgcctt ccgccagatg
    19201 atctggcggc agttgtcgca cttgacccag agtccttccg ttttaacgcg atgctcctct
    19261 tcggagggag cgccgggttg ccgggccggc tgttgtcggg taaaccaggc catgttgctt
    19321 ttagtattct atgcctcgct gcgcgaggac acccttgtgg aagggatgct tgatctcctt
    19381 gatctcgctg accagatcgg ccgcctccac cagtttcgga tgcgcattcc tgcccgtgca
    19441 gacgacatga agctgtcccg ggcgcgcaac caagccttcc aggacctggt cgattcccag
    19501 catcccgtaa tggatggcgt aattgatctc gtcgaggatc agcatatcgt acttcttgga
    19561 ctgcatcgcc tcgaccgccg cattccacgc ctcctgaacg aggcgcacat cctccggatc
    19621 cggctgttcg gcgccaactt ttacgaatcc gcgtcccatg acgcggatct cgaaggcgtc
    19681 cccaagcatt cctgccgagt cgagctcacc gtagtgccag gagcctttga tgaactggat
    19741 catcagcacc ttgaagccct ggccgacggc tcggaaggcg gttcccatcg cggccgaggt
    19801 cttacctttt ccattgccgg tgtagaccat cagcaggccg cgccgctctt ccatcgtttt
    19861 acgttagcgc gggtacggat ggccccgcaa cgtagtgaaa gcgcggtaga tctgttcagc
    19921 gagcatgacg cgggccaact cgtgcgggaa agtcattgaa gacaaagaga gaagcaggtc
    19981 ggcccgcggg cgccaggcgg cggggagacc gtcggcgccg ccaacgacga agaccagatc
    20041 gcgcgcctcg gcttcggctt tctcaatgag ggcggcaaac gcgggcgacc ccatcgtctt
    20101 cccggtgggg tcgagcagaa ttttggcggc gccggggtgt tttttccaca ggtcggcgcg
    20161 atccggattc acctcgcgca tttcgcattt cgcgtaccgc tgggtgcgtt tgaggaactc
    20221 ggcggccaag cggttggcat cgtgatcgcg tggcttgccg atgaagtaga tccagattcg
    20281 cggcacattt cattatgcgc cgtgggcggc ggtgttatcg tggagggcag cacacccccg
    20341 aatggtaaag acgcttctga tccgcgcccc ggaaggcaga tcccaggc
//
LOCUS       DATIFQ010000393        18475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2048566, whole genome shotgun sequence.
ACCESSION   DATIFQ010000393 DATIFQ010000000
VERSION     DATIFQ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 18475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18475
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048566"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VLE22_19200"
     CDS             complement(<1..304)
                     /locus_tag="VLE22_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16587.1"
                     /translation="MIRTLACLAVRGTFLLTACVALCDAHSIRVYSEFTRIDPLGHVV
                     KADRPRDPAVRPREVLSPALARNGFASFRVAVTVTPGEFFHLYVGENPENVFQITAY"
     gene            complement(301..522)
                     /locus_tag="VLE22_19205"
     CDS             complement(301..522)
                     /locus_tag="VLE22_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16588.1"
                     /translation="MKAEDYRERKLEVDGWPLNLTSYRAGGKYHCRADNVSPGAGLAR
                     TTGSTREEAEEKALARARELLARTRRQTV"
     gene            complement(527..1390)
                     /locus_tag="VLE22_19210"
     CDS             complement(527..1390)
                     /locus_tag="VLE22_19210"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HSB16589.1"
                     /translation="MGNYARPEVLVDTAWVTEHHRDPKVCIAEVDVDTSAYNEGHVPN
                     AIGWAWNTQLCDTVRRDILPKAQFEELMSASGITPDTTVVIYGDNNNWFAAWALWQMK
                     IYGHKDVRLMNGGRKKWLAEGRELSTESPVRKKVTYKAAAPDLSLRAFLPAAQSASKD
                     RTAALVDVRSPQEFTGEILAPPGLPETCQRGGHIPGARSIPWGKACNDDGTFKSYDEL
                     KRLYGAEGIDGSKPVIAYCRIGERSSHTWFVLKYLLGFEKVANYDGSWTEWGNLVGVP
                     VERGVPAAAGA"
     gene            complement(1495..3882)
                     /locus_tag="VLE22_19215"
     CDS             complement(1495..3882)
                     /locus_tag="VLE22_19215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HSB16590.1"
                     /translation="MGKELVISANRHETKVAVLEDGQLVEIYFQRANEYSLAGSIHKG
                     RVTRVLPGMQSAFVDVGLDRDAFLYVSDFFEENEEYDKIAAPDDRGRRGERERLERER
                     IERERAERVDRTEPAEPAPAEEKVEELPIPEHAAEPDGRDRKGRRSRRRRNRGRGFPE
                     SKYAPEPSVSWSPKEPEVQESAAAIEPLPGESLAKYRREEAGNGTILELAGAEEADAT
                     QINEPEAAGLVDPVPDAENGAAILEEPLAPEPAETQFEASEAEALIEETEDPANEAPE
                     DAMAEDPGVVEPQEPATVEEPVSESEPESEPAADDAPVETALESEPDEDTAEAAVPET
                     EEPSPALTAGVREREGRYPHRVSRRLRRKMRNAGPEPRDEEHAEPKAEARASSQPSES
                     PRQLPSITDLLREGQEVLVQVAKEPLGQKGARITSHIALPGRYVVYMPTVEHLGVSRK
                     IGSDEERQRLRRILMNHRVGIPGGFIVRTAGTGRSEQEIASDMTFLYNLWLDIRQRAE
                     RKPAPTLIHHDLDLVQRLLRDQLTESFKAIWVDHEDIYESVLRFVERLQPAMVGRVKM
                     YTRPTPIFDAFGVTAELEKALRPKVWLRSGGYIVINQTEALVAVDVNTGKFVGKSARL
                     EDTIVKTNTEAIKEIVRQIRLRDLGGIIVIDFIDMDERKNRQKVMQALEEEMRFDRAP
                     YKILQFNDFGLVAITRKRVKQTLERALCSPCPTCDGSGYVKSVQTVIGEILQEAQKIA
                     RAVEGPNVVLRVNPEVAKVLKSTDNDYLEELEEILRYPVLVTGDPELHPEKFDLA"
     gene            complement(4180..5271)
                     /gene="rodA"
                     /locus_tag="VLE22_19220"
     CDS             complement(4180..5271)
                     /gene="rodA"
                     /locus_tag="VLE22_19220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HSB16591.1"
                     /translation="MAKYLSARDLDWPLLALTVLICGLGILQIYSATLDTAWQDAWWK
                     QLLYVCVGLVVLYVSTRVDYHTLLEHVPLLYLLSLGTLVAVFLVGRQVFGSRRWIPLP
                     GGFTFQVSEFVKLVIVLLVARYLSELRSDRLEIRDLLKLGALVALPMVLVMKQPDLGT
                     SLTYGAILIAALFFGGIRWRYILVICLLALLALPVGWMFLKDYQKARLTSFQDPTQDP
                     RGSGYQVIQSKIAVGSGGMWGKGVTRGSQTQLRFLPVPHTDFIFSAFAEEHGFVGVVL
                     VLGLYFLLLMQIVQNAQMAPDRAGMFICMSVCSLLLFHLLVNVGMVVGRMPVTGIPLP
                     LMSYGGSSILSVFVMLGLVNNVRLRRFVN"
     gene            complement(5277..7151)
                     /gene="mrdA"
                     /locus_tag="VLE22_19225"
     CDS             complement(5277..7151)
                     /gene="mrdA"
                     /locus_tag="VLE22_19225"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSB16592.1"
                     /translation="MQLPLTDPTREDIPSRPLVRDDPKFASGRIAFFQYAAVAVFLFL
                     SSGFWDLQVSNPERYSEQAERNRIKALPVLAPRGKILDRDGRVIVDNHSSFSLILSRE
                     SLRPEHLQPIADGLHLEHEDLVGRLKRFRARPQYEPIVIKQELSAGELSFVEAHRDME
                     SFPEMELIHAQRRLYPRNGMAAHVIGYVGEVSDSELNTLEFVKYNPGDLIGKAGIERQ
                     YNDMLMGIDGQRQVVVDNRGREREVIGLKDSVSGKSLQLTIDLDLQAVAELAMENRKG
                     AVVALDPRNGEVLAMVSRPAFDPNRFADDWQELVGNPDKPLLNRAIQAQLAPGSTFKP
                     IVSLAALESGGVDDTFTVHCGGGASFYGRYFRCHLKGGHGQVDLHRGIVQSCDVYFYT
                     IGNRIGIDKIAEYASLAGLGKKTGIDLPHEAAGVVPSTEWKLRQFRQKWYAGETISVA
                     IGQGALTVTPLQLAYAIGGLAMGGAWYPPHLRMDLPQADRPRTANLNPESVARVIDGM
                     FGVVNEGGTGGRAQIPGVEVCGKTGTAQLASNEVLKNTKLGQTMKDNAWFVGFAPRQF
                     PEIVVVALFEGGEHGPMAAPIVRDVIKAYFDKKSRVSAPRMVAGAQPPVAQNATQGVQ
                     "
     gene            complement(7167..7700)
                     /gene="mreD"
                     /locus_tag="VLE22_19230"
     CDS             complement(7167..7700)
                     /gene="mreD"
                     /locus_tag="VLE22_19230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HSB16593.1"
                     /translation="MSEYQEPLYLGIAKRRRFSRFRAWVLFAVPLAAILFQVYVPLFF
                     QYGSYLDLPLIVTVYFSVTRRQPIGGILIGAGIGLVQDSLSHQPMGLFGITKTLCGYV
                     AASLGTRFEAEHGAVRFALGFVFFVLHQLAYWFLRGVLLGQAVDPEILRTLLLALLNG
                     AVTPPLFYLFDKLKTEG"
     gene            complement(7697..8923)
                     /gene="mreC"
                     /locus_tag="VLE22_19235"
     CDS             complement(7697..8923)
                     /gene="mreC"
                     /locus_tag="VLE22_19235"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HSB16594.1"
                     /translation="MDSLLGRYRNITVLLLVLFAQLILLAYQVRTDQDVRLIRIWAVS
                     AVTPVARVLEGARTATVHFFEDYLLFAKASEENRHLRAELGRLKMENQSLRSELGTAE
                     RAKLLSAYRERQPSRTVAARIIGTGAGSSSKVVFVDRGSADGVRRGMAVITPDGIVGK
                     VTESFPTASQVLLITDPTFAAGVVSENHRVMGTLKGTGKPDCIVDYLQIEERVDVGEW
                     FYTSGDDRVFPRGIPVGKVVSTGQGKVFKDVVVMPSGPRNGLEEVLIVLEGVHKPIPE
                     TSSASEGMYMLPAPLSEQQAASLPEDATTRELVFGTEADRLREHYRKVGEAQGHVYGE
                     GAAGSRPPDFNLKLSEPPAAGSPASGAVAAGPDVNAVAPPKAAKPPAVQPAKTSPAPQ
                     RSDSAESPAQRTTPAP"
     gene            complement(8942..9967)
                     /locus_tag="VLE22_19240"
     CDS             complement(8942..9967)
                     /locus_tag="VLE22_19240"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSB16595.1"
                     /translation="MNLRSLFSLFSSDLAIDLGTANTLVYARGKGIVVNEPSIVAINK
                     STGEVEAVGKEAKEMLGRTPGNIVAIRPMKDGVIADFKVTERMLTYFIHKAHGRRMLV
                     HPRIVIGVPSEITQVEKRAVIDSAYRAKASEVHLVEQAMVAAIGAGLPITEPSGNMVV
                     DIGGGTTDVAVISLSGIVYSRSVRVAGNEMDDAIMQYLKRKYNLLIGERTAEQIKIQV
                     GSAYPLEKPITMEIKGRNLIQGVPKTITVDDSEIRESLSECVATIMNAIRVALERTPP
                     ELSADISDRGIVLTGGGALLKNLDKRIREETGLPVSIAEDPLASVVLGTGKMLTDFRL
                     LRKISIE"
     gene            10266..11384
                     /locus_tag="VLE22_19245"
     CDS             10266..11384
                     /locus_tag="VLE22_19245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16596.1"
                     /translation="MLTRRYAIAACLSAAAGCGRRTASGFDGHAFVANEGSRAVAVVD
                     LSSFTLVRHIPFAAPPTALIPYPKTRAVYTLTPETGAVHEIDAARWTVKRKAQVADRA
                     ISMRLSPDVSALWVLCSQPAQLVRVPLESMREEARVPLPLEPVDFDLSAGGNLCAVSY
                     GEHGAVTMFPLAGHRAGRSVPIGGKAGIARFRSDGRYLIAANVADRIISILETPTGRV
                     VTHLPLAIQPERFCFKADGGQLFVSGSGMDAVVIVYPYSTEVDGTLLAGRAPGAMAVS
                     APPGPEYLFVANPQSGDVTVLDIETRRVIAVVTVGGEPGHVSVTPDSQYALVLSRGSG
                     NMAVIRIADVVPRRTRSAPLLAVIPVGSRPVSTAVLGV"
     gene            complement(11391..12665)
                     /locus_tag="VLE22_19250"
     CDS             complement(11391..12665)
                     /locus_tag="VLE22_19250"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HSB16597.1"
                     /translation="MKQAAILLQLVSLVCWGQAPYDLLLKGGHVIDPKNKIDARMDVA
                     VRDGKIAAVAAGIDPAQAKQVLDVEGLYVTPGLVDIHVHVFHTTGIKDAWAGDNSIPP
                     DAFSFRTGVTTMVDAGSSGWRNFETFRHTVIDRAKTRVLAMINIAGLGMMTDIVEQSD
                     FDPAEVARLAKKHRDVVVGVKSAHYQLPDWRSVERAVEAGAQAGIPVMVDFGYFLRER
                     PYWRLVTEQLRPGDITTHCFRGPVPWIDEDGKLYDYLYQARKRGVKFDVGHGGGSFVM
                     RNAAPAIRQGFYPDSISTDLHTGSMNGAMIDMPTTMSKLMALGLPLQNAVLCSTWTPA
                     QMIQRPELGHLTRDAVADVAVWRLLEGDFGFVDAAGGGIRGKQRLLCELTLKDGRISW
                     NWNARGAEDFRKLGPSYGIREGTDFIVPPPRR"
     gene            complement(12709..13569)
                     /locus_tag="VLE22_19255"
     CDS             complement(12709..13569)
                     /locus_tag="VLE22_19255"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 family protein"
                     /protein_id="HSB16598.1"
                     /translation="MRLPLIALLLALPLAVRPAGPFAFREAGPGSLELTENGKPVYVF
                     HHGMQWKQGAPEAMRRCCYLHPLYAPDGTAVTDDFPKDHYHHRGVFWAWPVVSAGGET
                     YDLWVLNGVGHRFVKWLSQEANGGFARLAAENGWFAGERKLVKETIEIVSRPARSNRR
                     ELDFTLSLEALGQPVQIAGQQDGKGYGGFCIRFGDREDTVVRTDTGREAKDTNLVPHP
                     WAELEGKFGGRRAGARIDIDQANPGFPNGWCLRYYGFLGVNFPGLKPYTLEPGKPVIL
                     KYRVTVYTVP"
     gene            complement(13566..14723)
                     /locus_tag="VLE22_19260"
     CDS             complement(13566..14723)
                     /locus_tag="VLE22_19260"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB16599.1"
                     /translation="MTRRQALSLVSAGLMRGATPAITRVEVFPVAYPVTAYFKFFPKP
                     ERPAVFVKITLEDGTTGWGQSVPTHTWSYETVESVVSTIEKYLAPVMIGRDPFDLAGA
                     HTAMNRAIAPSFSTGMPIAKAGIDLALHDLAGRLRRQSMPERWGRKPLPRITLSWTVN
                     PAAIGETEKLVEEGRARGYRNFNVKVAPDPKFDLELCRRVRKLAPEAFLWADANGGYD
                     LATALAVAPKLADAGVNVLEQPLPASRLTGYREIKKQGALPILMDEGVVSSAELVEFI
                     RLGLLDGVAMKPARTAGLWDARRQVEILLDAGLLILGSGLTDPDVALAAALQLYGAYS
                     LKFPAALNGPQFLRGSFLRKPLAVAGGEIDVPAGPGLGVEVDEDRVRRGTA"
     gene            complement(14761..15498)
                     /locus_tag="VLE22_19265"
     CDS             complement(14761..15498)
                     /locus_tag="VLE22_19265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HSB16600.1"
                     /translation="MARRRFFVETVRNGQAELTGDHAEHLRRVLRAERGQRFEISDNE
                     RVYLAEIEAFGKGQVQFRVLEEVAAETPAARFTLLVSLIKFDRLEWIVEKATELGAEV
                     IVPVKAERSEKGLDVAATKRLDRWRRIALESSQQSRRTRIPQVTEPLSFARTLSLEGD
                     YRYLLDEDTDAGAILEAAPAQRSAVDRVSLLVGPEGGWTESERKEAAEKQWRSVSLGP
                     QILRTETAAIAALAVLAAAWCTSSTSK"
     gene            15520..16116
                     /gene="ruvA"
                     /locus_tag="VLE22_19270"
     CDS             15520..16116
                     /gene="ruvA"
                     /locus_tag="VLE22_19270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HSB16601.1"
                     /translation="MIAYLRGTILEKHPNQVIVDAGGVGYDVTIPISTYSALPAEGAE
                     VRLRIHTHVREDALSLFGFLKREEKLLFEKLIDVSGIGPKLAVTVLSGLATADLIAAI
                     RQGQVELLVRIPGVGKKTAERMVLELRDKLDMLGPVPAVTAAPASSFTALEQDVVSAL
                     SNLGCARPTAETAVRKAKAAGVPVEFEPLFRKALELVR"
     gene            16147..17175
                     /gene="ruvB"
                     /locus_tag="VLE22_19275"
     CDS             16147..17175
                     /gene="ruvB"
                     /locus_tag="VLE22_19275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HSB16602.1"
                     /translation="MREKGIVSPAAQDDERQFEATLRPRRLDDFTGQTKLKENLAIAI
                     EAARQRGEAMDHVLLYGPPGLGKTTLATIISNELAVGFEQTSGPIVQKKLDLTGILSN
                     IRARQVFFIDEVHRLMPDVEEMLYAALEDFRVDILIGSGPGARTHSLPIPRFTAIGAT
                     TRQGLVSAPLRGRFGLVLRLNHYDVGELKKIVLRSAGLLEVEIEDAGAEEVARRSRGT
                     PRIANRLLRRVRDYAQVRADGRVSREIAVTALDLLEVDRFGLDEIDQKIMLTILEKYG
                     GGPVGLNTIAASIDEEPDTIEEVYEPYLLQLGFLDRTPRGRLATDRAFEYFGVPRRMR
                     GGAQNTLF"
     gene            17251..17736
                     /gene="folK"
                     /locus_tag="VLE22_19280"
     CDS             17251..17736
                     /gene="folK"
                     /locus_tag="VLE22_19280"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HSB16603.1"
                     /translation="MKTVYLSLGSNVGNREEMLQQAVRLLASADFRITRMSSIYETEP
                     QDVRGQPWFLNMVLEAETTLFPRQLLGRIQKVEQQLGRRRLAPKGPRTIDIDILLFGT
                     AVIDTAGLTVPHPRMAERRFVLEPMTELEPDLHHPILRRAMRELLGTVRDQTVRRKTG
                     D"
     gene            complement(17759..>18475)
                     /locus_tag="VLE22_19285"
     CDS             complement(17759..>18475)
                     /locus_tag="VLE22_19285"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HSB16604.1"
                     /translation="TADNLFDYMNGNAEGYVIYSFHKMDGVTCKSGENTFVFDVSEMG
                     SPELAYGIYTANRDIRRPAETIGMGGQIQPRRAIFAKDKYFVEIAASPDKDHTASLRQ
                     FVEAMEKRIQGSTKLPAALGWFPAEKLVKDSVRLVPQSVLGMRALRRGYVAEYEFGKA
                     FIATESSPDAAAASIEKVKARIGEGEPVAIADGGFQVNSRYLGRVCVFRKGSYLAGYA
                     NLAEGVDGVALAKTLAERIP"
BASE COUNT         3403 a         5947 c         5531 g         3594 t
ORIGIN      
        1 ggtacgcggt gatctggaac acgttctctg gattctcgcc gacgtacaga tggaagaact
       61 cgcccggagt caccgtcacc gcgacccgaa atgaagcgaa cccgtttcgg gccagcgccg
      121 gcgacaggac ctcgcgcggt cgcacagccg ggtcacgcgg acggtctgcc ttaaccacgt
      181 gtccgagcgg atcgatccgc gtgaactcgg agtacacgcg aatgctgtgc gcgtcacaca
      241 gcgcgacaca tgccgtcagc aggaatgtgc cccgcacggc gaggcaggca agcgttctga
      301 tcacaccgtc tggcggcgcg tccgcgcaag caactcccgg gcgcgcgcga gggctttctc
      361 ctctgcttct tccctggtcg atccggtcgt cctggcgagg cctgcgccgg gggacacatt
      421 atccgcccgg cagtgatact ttccgcccgc gcgatagctg gtcaaattca gcggccagcc
      481 atccacttcg agcttccgtt cgcggtagtc ttcggctttc atcgtttcag gctcccgccg
      541 ccgcaggtac accccgctca accggaacgc cgaccaggtt gccccactcg gtccaagaac
      601 catcgtagtt cgcgactttc tcgaatccaa gcaggtactt gagcacgaac cacgtgtgac
      661 tggaacgctc gccgatccgg cagtacgcga tcacaggctt cgaaccgtcg atgccctcgg
      721 caccatacaa gcgcttcagt tcgtcgtaag acttgaacgt cccgtcatcg ttgcatgcct
      781 tcccccatgg gatactgcgg gcgcccggga tgtgaccccc tctctggcat gtttcaggca
      841 gtcctggcgg cgcgagaatc tcgcccgtga actcctgtgg gctccgcaca tccacaagcg
      901 ccgccgtccg atccttcgaa gcggactggg ctgccggcag aaacgctcgc agcgaaagat
      961 ccggcgcagc cgctttatac gtcactttct ttcgcacagg cgactccgtc gagagttcgc
     1021 ggccctcggc aagccatttc tttcggccgc cgttcatcag gcggacgtcc ttgtggccgt
     1081 aaatcttcat ctgccaaagc gcccatgcgg cgaaccagtt gttgttgtcc ccatagatga
     1141 cgacggtcgt gtcgggagtg atgccggacg ccgacatcag ctcctcgaac tgtgctttcg
     1201 gcaggatgtc ccgccggacc gtgtcgcaga gctgcgtatt ccaggcccag ccgatcgcgt
     1261 tcggtacgtg gccctcgtta taggcggagg tgtcaacgtc cacctcggcg atacacactt
     1321 taggatcacg atggtgctca gtaacccagg cggtatcgac gagtacttcc gggcgagcgt
     1381 agttccccat ttttgattcc tcctctgagt ggatataccc aaaactatat aagcaatagg
     1441 gactacgcgc aagccccccc ttacgggagg cttttagcgg cgggtttctc aatactaagc
     1501 gaggtcgaac ttctccgggt ggagctccgg gtcgcctgtg accaggactg gatagcgcag
     1561 gatctcctcc agctcttcca gatagtcgtt gtcggtcgat ttcagcacct tggcgacttc
     1621 cggattcact ctcaagacga cgttcggccc ctcgacggcg cgggcgatct tctgagcttc
     1681 ttgaaggatc tcaccgatta ccgtctgcac actcttgacg taaccggagc cgtcgcacgt
     1741 cggacacggc gagcacagtg cgcgctcgag tgtttgcttc accctcttgc gggtgatggc
     1801 aacgagtcca aagtcgttga actggaggat cttgtacggt gcccggtcaa accgcatctc
     1861 ctcttcgagc gcctgcatca ctttctggcg gttcttgcgc tcatccatgt cgatgaaatc
     1921 gatgacgatt atgccgccga ggtcccgcag ccggatctgc cgaacgattt ccttgatcgc
     1981 ctcggtgttg gtcttgacga tcgtgtcttc gagccgggcg gacttcccca caaatttccc
     2041 ggtgttgaca tcgactgcca ccagcgcttc ggtctgattg atcacgatgt aaccgccgga
     2101 cctcagccat accttggggc gcagcgcttt ctccagttca gcggtcacgc caaacgcgtc
     2161 gaaaatcggc gtcgggcgcg tgtacatctt gacccggccc accatggccg gctgcagccg
     2221 ctccacaaac cgaagcacgc tctcgtagat gtcctcgtgg tccacccaga tcgccttaaa
     2281 actctcggta agctggtctc ggagaagccg ctgtaccaga tcgagatcgt ggtggatcag
     2341 cgtcggggcc ggtttccgct cagcccgctg gcggatgtcg agccagaggt tgtacagaaa
     2401 ggtcatgtct gaggcgatct cctgctcgga tcgcccggta cccgcggttc tgacgataaa
     2461 gccgccaggg atgccgacgc ggtggttcat gagaatacgc cgaaggcgct gacgttcttc
     2521 atcggaaccg atcttccggg acacgccgag atgctcgaca gtcggcatgt aaacgacgta
     2581 gcggccgggc agagcaatgt gcgaggtgat gcgcgcgccc ttctgcccaa gcggttcctt
     2641 ggccacctgg acgagaacct cctgtccctc gcgcaggagg tcggtgatgg acggaagctg
     2701 gcggggggat tcggatggct gtgaggacgc tcgggcctcc gccttcggct cggcgtgctc
     2761 ttcgtcacgc ggttcgggtc cggcattacg catcttgcgc cgcaaccggc gcgacacccg
     2821 gtggggatag cggccttctc gctcgcgaac gcccgcggtc agggcaggag agggttcctc
     2881 agtttcaggg acggccgcct ccgcggtgtc ctcgtccggc tcggactcca gagcggtttc
     2941 gacgggtgcg tcgtcggcag cgggctcgga ttccggctcg gactccgaga caggctcctc
     3001 aaccgtcgcc ggttcttgtg gctcgactac ccctggatcc tcagccatcg catcttcagg
     3061 agcttcgttt gccgggtctt ccgtctcttc gatgagagcc tctgcctccg aggcttcgaa
     3121 ttgtgtttcc gccggctccg gcgcgagtgg ttcttccaag atggcggcgc cgttctccgc
     3181 gtccggcacg ggatctacga ggcccgcggc ctccggttcg ttgatctggg tcgcatccgc
     3241 ttcttcggcg ccggctagct ccaggattgt gccgtttcct gcctcctccc ggcggtactt
     3301 cgccagggac tcgccgggaa gcggttcgat cgccgcggcg ctttcttgca cttccggttc
     3361 cttgggagac cacgacaccg acggttccgg ggcgtacttc gattcgggga acccccggcc
     3421 gcgattccgg cgccgccgtg aacgccggcc tttgcggtcg cgcccgtccg gttcggcagc
     3481 gtgttccgga atgggcagtt cttcgacctt ctcctcggcc ggggcgggct ccgccggttc
     3541 cgtcctatcg acgcgctcgg ctcgttcccg ttcgatccgc tcccgctcca agcgttcacg
     3601 ctctcctcgg cggccgcgat cgtcgggtgc cgcgatcttg tcgtactcct cgttctcttc
     3661 gaaaaaatcg gagacgtaca aaaaggcgtc acgatcgagg ccgacatcga cgaaagccga
     3721 ttgcataccc ggcagcacac gggtaacacg acccttgtgg atgctgcccg caagcgagta
     3781 ttcgtttgcc cgctgaaagt agatctcgac caactggcca tcttccagaa ctgccacttt
     3841 cgtttcgtgg cggttggccg aaatcaccaa ctcttttccc atgaaatcct ccgcgtcgga
     3901 agcgcgggcg aggcgacggg aagaaatgcg gccggggctg tagagggacc tcagttctgc
     3961 gacggcgctg gagtaccgga cattgctccg gctcctgcgt caaaaccttt cttccggcgg
     4021 cggggatgga gccgctttgc tcaaacatcc gcccacccac cggggtgctg caccgaacct
     4081 cgggaccgcg tcccttgccg tgcaggaacc tcagtatccc tctcaacaac ggaaccttgc
     4141 caccttcaga cttcccgcga aacagtgtct cgcaacgctt caatttacaa accgccggag
     4201 cctgacgttg ttgaccagcc ctagcatcac aaataccgac agaatgctag agccgccgta
     4261 gctcatcagc ggcaatggga tgccggtgac cggcatccgg ccaaccacca tcccaacatt
     4321 caccaacagg tgaaacagca acaatgaaca cacgctcatg cagataaaca tgccggcccg
     4381 gtccggggcc atctgtgcgt tctgtacgat ctgcataagc agcaaaaaat acaagccgag
     4441 tactaatact accccaacaa aaccatgctc ttccgcaaaa gccgaaaaaa tgaagtccgt
     4501 gtgtggcacc ggaaggaagc gaagctgtgt ttgagaaccc cgggttacac ccttccccca
     4561 catgccgcca gaaccgacgg cgatcttgga ctggatcacc tggtagcctg agccacgcgg
     4621 gtcctgggtc ggatcctgga atgatgtcag gcgggctttt tgataatcct tcagaaacat
     4681 ccacccgaca ggcagagcca gtagggcgag caggcagatc acaagaatat accgccagcg
     4741 aattccccca aaaaacaagg ccgctatcag gatcgcgccg taggtcagcg acgtgcccaa
     4801 gtccggctgc ttcatcacaa gcaccatcgg aagcgcgact agcgccccga gcttcagcag
     4861 atccctgatt tccagcctgt cgctcctcag ttcgctcaaa taccgcgcca cgagcaatac
     4921 tatgaccagt ttaacgaatt cggagacttg aaatgtgaat ccgcctggga gcgggatcca
     4981 ccgccgggag ccgaacacct gacggcccac caggaacacc gcgaccaggg tccccagcga
     5041 cagcaagtag agcagcggta cgtgttcaag cagagtgtgg tagtcgactc gggtcgacac
     5101 gtacagaacc accaaaccta cgcagacgta tagcaactgc ttccaccagg cgtcttgcca
     5161 ggccgtgtcg agggttgcac tgtagatctg aaggatgccc agaccacaga tcaacaccgt
     5221 cagcgccaga agcggccagt cgagatctcg ggctgagagg tatttcgcca tgccggttac
     5281 tgcactccct gtgtggcgtt ttgcgcgacg ggaggctgcg cgcccgcgac cattcgagga
     5341 gcggagacgc gcgacttctt gtcgaagtac gctttgatta catcgcggac gattggcgct
     5401 gccatgggtc cgtgctcacc gccttcgaag agggcgacca ccacaatctc gggaaactgg
     5461 cgcggagcga aaccgacgaa ccacgcgtta tccttcatcg tttggccgag cttggtgttc
     5521 ttaagcacct cgttggaagc aagctgggct gtcccggtct tgccgcagac ctcaacaccc
     5581 gggatttgcg cccggccgcc ggtgccgccc tcattgacga caccgaacat cccgtcaatt
     5641 actctcgcca cgctttcggg gttcagattc gccgtcctgg gccggtcggc ctgcggcaga
     5701 tccatacgca gatgaggcgg gtaccaggct ccgcccatcg ccaagccgcc aatcgcgtat
     5761 gcgagctgga gcggagtgac cgtcagagcg ccctgtccga ttgccaccga aatcgtctct
     5821 ccggcgtacc atttttgccg gaattgacgc agtttccatt ccgtcgacgg aaccacgccc
     5881 gccgcttcgt gcggaaggtc gatgccggtc ttcttcccga gtccggccag gctcgcgtat
     5941 tcggcgatct tgtcaattcc aatcctgttg ccgatggtgt agaaataaac gtcacaagac
     6001 tgcacgatgc cacgatgcag gtcaacctgt ccgtgcccgc ccttaaggtg acaccggaag
     6061 taacggccat agaaactcgc tccgccccca cagtgtacgg tgaaggtgtc gtccactccg
     6121 ccggattcga gtgcggccaa tgatacgatc ggcttgaaag tagacccagg cgcgagttgc
     6181 gcctggatgg ctcgattcag cagcggcttg tcggggttgc cgacgagttc ctgccagtcg
     6241 tccgcgaatc tgttgggatc gaaggcgggg cggctcacca tcgccagcac ctcgccgttg
     6301 cgggggtcga gggccaccac agcccctttc cggttctcca tggccagttc cgccaccgcc
     6361 tggaggtcga ggtcgatcgt gagttgcagc gacttaccgg agaccgagtc cttgaggcca
     6421 atcacctcgc gttccctgcc ccggttgtcg acgaccacct ggcgttggcc gtcaatgccc
     6481 atcaacatgt cgttatactg gcgctcgatg ccagccttgc cgatgaggtc tccggggttg
     6541 tatttgacga actccagagt gttcagttcg gagtcgctta cctcgccgac gtagccgatc
     6601 acgtgcgccg ccatcccgtt acgcgggtac aggcggcgct gcgcgtggat cagttccatc
     6661 tcagggaagc tttccatgtc gcgatgcgcc tctacgaatg acaactcgcc ggcgctcagc
     6721 tcctgcttga tgacgatcgg ctcgtactgc ggccttgcac ggaagcgctt caggcggccg
     6781 accaggtctt cgtgttccag gtgaagtccg tcggcgatcg gctggaggtg ctcgggccgg
     6841 agactctccc gggacagaat gaggctgaac gaggaatggt tatcgacgat gacccgccca
     6901 tcacgatcga ggatttttcc gcgaggcgcg aggaccggca acgccttgat tcgattccgt
     6961 tccgcctgct cgctataacg ctcgggattg ctcacctgga ggtcccagaa accggaactg
     7021 agaaaaagaa aaacggctac cgcggcgtac tggaaaaacg cgatccgccc cgatgcgaac
     7081 ttcgggtcgt cccgcaccag cggcctggat gggatgtcct cgcgcgtggg gtccgtcaac
     7141 ggcagttgca cgatgccaac agttctctac ccttcagtct tgagtttatc aaagaggtag
     7201 aacaggggcg gtgtcacggc gccgttcagc agcgcgagca gaagtgttcg tagaatctcc
     7261 gggtcgactg cctggcccag caagacaccg cgcaaaaacc aatacgcgag ttgatggagg
     7321 acaaagaaca cgaaccccag tgcgaatcgc acggcgccgt gttccgcttc gaatctcgtc
     7381 ccaagcgatg ccgcaacata accgcaaagc gtcttggtta tgccgaacag tcccatcggc
     7441 tggtgtgaga gggagtcctg gacgaggccg atgccggcgc caatgagtat cccgccgata
     7501 ggctgtcgcc gggtcacaga gaaatacacc gtgacgataa gaggaaggtc gaggtaggag
     7561 ccgtactgga agaacagcgg gacgtacacc tgaaagagga tcgctgcgag cgggactgca
     7621 aatagcaccc atgcgcggaa gcgcgagaaa cgcctccgct tcgcaatgcc gaggtacaaa
     7681 ggttcttggt actcgctcat ggcgcggggg tcgttcgctg cgcggggctt tcggcggaat
     7741 ccgaccgttg cggtgcgggg gaggtcttcg ccggctgaac cgccggtggc ttcgcagcct
     7801 ttgggggcgc gactgcgttc acgtcgggcc cggccgccac ggctccagac gccggcgagc
     7861 cggccgcggg cggctcgctg agcttcagat tgaagtccgg cggcctggat cctgcggcgc
     7921 cttcgccgta cacatgtccc tgcgcttctc ctaccttccg gtagtgctct cgcagccggt
     7981 cagcttcggt cccgaagaca agttcccttg ttgtggcatc ctcggggagc gaagcggcct
     8041 gctgttcgga cagcggcgcc ggaagcatgt acatgccctc ggacgcgctg ctggtctccg
     8101 ggatcggctt gtggacgccc tcgaggacga taagcacctc ctcaaggccg ttccgcgggc
     8161 cgctcggcat caccacaaca tccttgaaca ccttgccttg acccgttgaa accaccttgc
     8221 cgaccggaat gccgcgcggg aacacccggt cgtcgccgga cgtgtagaac cactcgccta
     8281 catcgactcg ctcctcgatc tggaggtagt ccacaatgca gtccggcttt cccgttccct
     8341 tgagggttcc cattacgcgg tgattctcgc tgaccacgcc cgcggcgaag gtgggatcgg
     8401 tgatcagtaa gacttgcgaa gcggtaggga aactctcggt caccttgcct acgatgccgt
     8461 cgggggtgat gaccgccatg ccgcgacgga ccccgtcggc cgaaccccgg tcaacgaaca
     8521 ccaccttgga actggaaccg gctccggtcc cgatgatccg ggcggcgacg gtgcgcgatg
     8581 gctggcgctc ccggtacgca ctgagaagct tggcgcgttc ggcggtcccg agttcagacc
     8641 gcagagattg gttctccatc ttgagacgac ccagttcggc ccgaagatgc cggttttcct
     8701 cgcttgcttt cgcgaacagc aggtagtctt cgaagaagtg caccgtagcc gtacgagcac
     8761 cctcaagtac gcgggcgacc ggggtaacgg cactgaccgc ccagatccgg atcaggcgta
     8821 catcctggtc cgtccggacc tgatacgcga gcagaataag ctgcgcaaac aacacgagca
     8881 gcagcaccgt aatgtttcgg taccggccaa gcaaagagtc catgccattc tcaagttggc
     8941 ttcactcgat cgaaatcttg cgaagcagcc tgaagtcggt cagcattttt ccggtgccca
     9001 gcaccaccga ggcgagcgga tcctcggcga tcgagacggg gaggcctgtc tcctcccgga
     9061 tgcgcttgtc gagattcttg agcaaagccc caccaccggt gaggacaatg ccgcggtcgc
     9121 tgatgtcggc gctcaactcg gggggcgtgc gctccagcgc cacgcgaatt gcattcatga
     9181 tcgtggcgac gcattccgac aacgactcgc ggatctcgct gtcatccacg gtgatcgtct
     9241 tgggcacgcc ttgtatgagg ttgcggccct tgatctccat ggtgatcggc ttctcaagtg
     9301 ggtaggctga gccgacctgt attttgatct gctcggcggt gcgctctccg atcagaaggt
     9361 tgtattttcg cttcaggtac tgcatgatcg cgtcgtccat ctcgttgccc gctacgcgca
     9421 ccgaacggga atacacgatg ccagaaagtg agatgacggc gacgtcggtg gtgccaccgc
     9481 cgatgtcgac caccatgttg cccgagggtt cggtgatcgg caggccggcc ccgatggcgg
     9541 ccaccatcgc ttgctccacg agatgcactt cgctcgcctt ggcgcggtac gccgaatcga
     9601 ttacagcgcg cttctccact tgtgtaatct cgcttggtac gccgatcacg attctcgggt
     9661 ggaccaacat ccggcggccg tgcgccttgt ggatgaagta cgtcagcatc cgctccgtaa
     9721 ccttgaagtc ggcgatcacg ccgtccttca tcggacggat ggccacgata ttgcccggcg
     9781 tgcgtccgag catctccttg gcttccttgc ctacggcttc cacctcgccg gtcgacttgt
     9841 tgatggcgac gatcgagggt tcgttgacga caatcccctt ccctctggca taaacgagcg
     9901 tgttcgctgt ccccagatcg atcgcgaggt cggacgaaaa aaggctgaaa agagaacgaa
     9961 gattcattac aacctgaact cagtgttgtc gattattgtg cgaatctacc tcgctaaccc
    10021 ggtttcccat tcgttgatga cgcccaacac tgtgttgcgg gaaaggggga agggtagtcc
    10081 cctcaaaggc taacataatg gcccgcctgc ttcaatgacc actaagtgta tgattttcaa
    10141 tggacaggat agcgcgagag cagcagggag tctccccgat ttcttccaca actcgctgtc
    10201 cctcaagatc ggtgggccga ggcggcttgg tgaccgtagg ccggtccgtc tacaatggat
    10261 cgtctgtgct gactcgacgc tacgcgatag ccgcctgcct ttcggccgcc gccggctgcg
    10321 gccgccgtac ggcatcgggc ttcgacggtc acgcgtttgt tgcgaacgag ggaagccggg
    10381 cggtagcggt tgtggatctg tcgagtttca ccctcgttcg gcacatcccc ttcgcggcgc
    10441 ctccgaccgc gttaataccg taccccaaga ccagagctgt ctatacgcta accccggaga
    10501 cgggcgcggt tcatgagatt gacgcagccc gctggacggt gaagcgcaag gcgcaggtgg
    10561 cggacagggc aatctcaatg cggctttcac cggacgtcag tgccctctgg gtgctttgca
    10621 gccaaccggc tcaattagtg cgggtacccc tggaaagcat gagggaagag gccagggttc
    10681 ctctgccgct ggaaccggtc gatttcgacc tttcggcggg tggcaatctg tgcgccgtga
    10741 gctacggcga acacggcgcc gtgacgatgt tccctctcgc cggccaccgg gccggccggt
    10801 cggtcccgat tggcgggaag gcgggaatcg cacggttccg gtctgacggg cgttacctga
    10861 tcgccgctaa tgtagcagac aggatcatct cgatccttga gacgccgacc ggccgcgtgg
    10921 tgacgcactt accgctcgcg attcaaccgg agcgtttctg tttcaaggcg gatggcggcc
    10981 agttgtttgt gagtgggtcc ggaatggacg cggttgtgat cgtctacccg tacagcaccg
    11041 aggtggacgg cacattgctc gccggccggg cgccgggggc gatggccgtc tcggcgccgc
    11101 ccggcccgga atacctgttc gttgccaacc ctcagtcggg agacgtgacc gtgctcgaca
    11161 tcgagacccg acgcgtgatt gcggtggtga cggtgggcgg ggaacccggc catgtctcgg
    11221 tgactccgga cagccagtac gcactagtcc tcagccgggg gtcgggaaat atggcggtga
    11281 tccggatcgc ggatgttgtg ccgcgccgca cgcggtcggc ccctctgttg gccgtcatcc
    11341 ccgtgggctc caggcccgtc agcaccgcgg tgctcggcgt ctgatctcct ctaccggcgg
    11401 ggcggcggca cgatgaaatc ggttccctcc cgaatgccgt aggatggccc aagcttccga
    11461 aagtcttccg cgccacgcgc attccagttc caggagattc gcccgtcttt gagtgtcaac
    11521 tcgcacagca ggcgctgttt cccgcggatc ccgccgccgg cagcgtcgac gaaaccgaaa
    11581 tcgccttcca gcagcctcca gacggccaca tcggccaccg cgtcccgcgt gagatgtccc
    11641 aattcgggcc gctgaatcat ctgggcgggc gtccaagtcg aacagagcac cgcattctgg
    11701 agcggcagcc caagcgccat cagcttggac atcgtggtag gcatgtcgat catcgcaccg
    11761 ttcatactgc cggtgtgaag atcggtggaa atcgagtccg ggtagaaacc ctgccgaatg
    11821 gcaggcgcgg cgttgcgcat cacaaaactt ccgccgccat gaccgacgtc gaacttcacg
    11881 cctcgtttcc gggcctgata aagatagtcg tacaacttac cgtcttcgtc gatccacggc
    11941 acgggaccgc ggaagcagtg cgtcgtaatg tcgccgggcc gtagctgctc cgtcaccagt
    12001 cgccaatacg gccgctcgcg cagaaaatag ccgaagtcga ccatcaccgg tatgccggct
    12061 tgcgctccgg cctcaaccgc ccgttccacc gagcgccagt cgggtaactg gtagtgcgcc
    12121 gacttcacgc cgaccacaac gtcccgatgc ttcttcgcca gacgcgcaac ctcggccggg
    12181 tcgaaatcgc tctgctcgac gatgtcggtc atcatgccga gcccggcgat gttgatcatc
    12241 gccagcaccc gcgtcttcgc acggtcgatc accgtgtgac ggaacgtctc gaagttgcgc
    12301 cagcccgaac tgccggcgtc caccatcgtc gtcacaccgg tccggaagct gaacgcatcc
    12361 ggcggaatgc tgttatcgcc ggcccaggca tccttgatgc cggtggtatg aaacacgtgt
    12421 acatggatat ccaccagccc gggagtgacg tagaggccct ccacgtccag aacctgcttt
    12481 gcctgcgcgg gatcaatgcc ggcggcgacc gccgcgatct tcccgtcccg tacggcaacg
    12541 tccattcgcg cgtcgatctt gttcttcgga tcgatgacgt gaccgccctt caacagcagg
    12601 tcgtacggcg cctgccccca acagaccaga gaaaccagtt gaagaagaat cgctgcttgt
    12661 ttcatgtcct catgccttgt tgtttactgc cggctccatc gcgcttggct atgggaccgt
    12721 gtacactgtg acgcggtact tcaagatgac cggcttgccg ggctcgagcg tgtacggctt
    12781 cagtccgggg aagttcacgc cgaggaaacc gtagtaacgc aggcaccatc cgttcgggaa
    12841 cccggggttg gcctggtcaa tgtcgatgcg cgcacccgcg cgacggccgc cgaactttcc
    12901 ctcgagctcc gcccacgggt gcggaacgag gttcgtgtcc tttgcctcgc ggcccgtgtc
    12961 ggtccgcacg accgtgtctt cacggtcgcc gaaccgaatg cagaaaccgc catagccctt
    13021 cccgtcctgt tgcccggcga tctggacggg ttgcccaagt gcctcgagcg agagcgtgaa
    13081 gtcgagctca cgccggttgg atcgagccgg acgcgagacg atctcgatgg tttccttcac
    13141 cagttttcgt tcgccagcga accagccgtt ttcggccgcc agccgcgcga agccgccgtt
    13201 ggcctcttgg ctaagccatt tcacgaatcg gtggcccacg ccgttcagca cccacaggtc
    13261 gtacgtctcg cctccagcgc tcaccacagg ccacgcccag aagacgccac ggtgatgata
    13321 gtggtctttc gggaaatcgt ccgtgacggc cgtgccgtct ggtgcgtaca acggatgcag
    13381 atagcagcac cgccgcatcg cctccggggc gccttgcttc cactgcatgc cgtggtggaa
    13441 gacgtacacc ggcttcccgt tctctgtcag ctcgagcgaa cccggtcccg cctcgcgaaa
    13501 agcaaaaggg ccggcgggcc ggactgccaa gggtaacgca agcagcaaag cgatgagcgg
    13561 caatctcatg cggttcctcg tcgaacgcga tcctcgtcca cctcgacacc gagccccggc
    13621 cctgcgggga cgtcaatttc accgcccgcc acggcaagcg gcttgcgcag gaagctcccg
    13681 cgcaggaact gagggccatt gagcgccgcc ggaaacttca gactgtaggc gccgtacagt
    13741 tgcagcgcgg cggcgagcgc cacgtccggg tcggtgagcc cgctcccgag aattagcagg
    13801 ccggcgtcca gcaggatttc cacttggcgc cgtgcatccc acaggccggc ggtccgcgcg
    13861 ggcttcatcg caaccccgtc gagcagcccg agccggatga attcgaccag ttccgccgaa
    13921 gagaccacgc cttcgtccat caggatcggg agcgcgcctt gcttcttgat ttcgcgataa
    13981 cccgtgaggc ggctggccgg caacggctgc tccagcacat tcacgccggc gtccgccagt
    14041 ttcggagcaa cggcgagcgc ggtggccagg tcgtagccac cgttggcgtc ggcccagaga
    14101 aatgcctcgg gcgcgagctt ccgcacgcgc cggcacaact cgagatcgaa cttcgggtca
    14161 ggagcaacct taacgttgaa gtttcggtaa cctcgggcgc ggccctcctc gacgagtttc
    14221 tcggtttcgc cgatcgccgc cggattcacc gtccagctca gagtgatccg cggtagcggc
    14281 ttgcgtcccc accgctcagg catgctctgc cgccgcagac gtccggcgag gtcgtgcagc
    14341 gcaaggtcga tgcccgcctt ggcgatcggc atcccggttg agaaggaggg cgcgatcgcc
    14401 cggttcatgg ccgtgtgggc gccggcaaga tcgaacgggt cgcgcccgat catgacgggc
    14461 gccaggtatt tctcaatggt cgagaccaca gactccacgg tttcgtagct ccacgtgtga
    14521 gtcggaacgc tctgccccca cccggtggtt ccgtcttcca aagtgatctt cacgaacaca
    14581 gctgggcgct cgggtttcgg gaagaacttg aaataggcgg tcaccgggta cgcaaccgga
    14641 aatacctcga ctcgggtgat ggcgggcgtt gccccgcgca tgagtccagc ggacacgagg
    14701 ctcagtgctt ggcggcgggt catctccaca gcaaagaatt ataagcgcag cagcccctgg
    14761 tcatttagag gtcgagctgg tacaccaggc ggcagccaat accgccagcg ccgcgatggc
    14821 ggccgtttca gtccgcagta tttgtggacc gagcgagacg gaccgccact gtttctcagc
    14881 cgcttccttg cgttcgctct ccgtccagcc tccctcgggt cccacgagca gggaaaccct
    14941 gtccaccgca gagcgctgcg cgggcgccgc ttccaggatc gccccggcat cggtgtcttc
    15001 atcgagcagg tatcggtagt cgccttcaag acttagcgtc cgagcgaagg acagcggctc
    15061 cgttacctgc ggtattctcg tgcgtcgcga ctgctggctg ctttccagcg caatccggcg
    15121 ccagcggtca agccgcttcg ttgcagccac gtccagcccc ttctcgcttc gttccgcctt
    15181 cactggaacg atgacctcgg cgccgagctc ggttgccttc tcgacgatcc actcgaggcg
    15241 gtcgaacttg atcagggaga cgagcagcgt gaatcgcgcc gccggagtct cggcggcgac
    15301 ttcctccaac acgcggaact gaacctgtcc tttaccgaac gcttcgattt ccgccaggta
    15361 gacccgctcg ttgtcggaaa tctcgaaccg ctggccacgc tccgcccgca gcacgcgccg
    15421 caggtgctcc gcgtggtccc cggtcagttc cgcctgcccg ttgcgtactg tctcgacaaa
    15481 gaaacggcgt ctcgccatgt acaataatct acgcctgtca tgatcgcgta tctgcgcggc
    15541 accatcttag agaagcaccc gaaccaggtg atcgtcgacg ccggtggtgt cggctacgat
    15601 gtcaccattc ccatttcgac ctactccgcc cttccggccg aaggcgccga ggtgcggctt
    15661 cgcatccaca ctcacgtccg cgaggatgcg ctgtcgctct ttggttttct caagagagaa
    15721 gagaagctgc tgtttgagaa gctgatcgac gtgagcggca tcgggccgaa acttgcggtg
    15781 accgttctgt ctgggctggc taccgccgac cttatcgctg caatccggca aggacaggtt
    15841 gagctcttgg tacgcatccc cggagtgggc aagaaaacgg cggagcggat ggtcctcgaa
    15901 ctccgcgaca aactcgacat gctcggaccg gtgccggcgg tgacggctgc cccggcgtcg
    15961 tcgttcactg cgctcgagca agacgtcgtt tcggcgctga gcaatcttgg ctgcgcacgc
    16021 ccgacggcgg agacggcggt acggaaggcg aaggcagccg gtgttccggt cgaattcgaa
    16081 ccgctctttc gcaaggccct cgagcttgtg cgctagcggc ggacggacca agtaaactaa
    16141 tacacaatgc gcgagaaggg aatcgtttcg cccgccgcac aggacgacga gcgccaattc
    16201 gaagccaccc tgcggccgcg ccggttggat gatttcaccg gccagaccaa gctgaaggaa
    16261 aaccttgcca tcgcaatcga ggctgcccgg cagcgtggcg aggcgatgga ccatgttctg
    16321 ctctatggtc cgccaggcct cggcaaaact acgctcgcca cgatcatttc caatgaactg
    16381 gcggtcggct tcgagcagac ctctggcccg atcgtgcaga aaaagctcga cctcaccggc
    16441 attctgagca acatccgggc gcgccaagtc ttcttcatcg atgaggtcca ccgcctgatg
    16501 ccggatgtcg aggagatgct ctatgctgcc ctcgaagatt tccgggtcga catcctgatc
    16561 ggcagcggcc cgggcgcgcg cacgcattcc ctgccgatcc cgcgtttcac ggccatcggg
    16621 gcgaccacgc gccagggtct cgtgagcgcg ccgcttcgcg gccgtttcgg gctggtccta
    16681 cgtctgaacc actacgacgt cggagagctc aagaagatcg tgctccgttc ggcgggcctg
    16741 ctggaggtcg agattgagga cgccggcgcg gaggaggtcg cgcggcggag ccggggcacc
    16801 cctcgtatcg ctaatcgcct gcttcgccgg gttcgcgatt acgcacaggt tcgcgcggac
    16861 gggcgggtct cgcgcgagat tgccgtgacg gcgctggatc tactcgaggt cgatcgtttc
    16921 ggcctcgacg agatcgacca gaagatcatg ttgacgattc tcgaaaagta cggcggcggt
    16981 ccggtcggtc tcaacaccat cgcggcgtcg atcgacgaag agcccgacac catcgaggag
    17041 gtgtacgaac cctacctgct tcaactcggc ttcctcgacc gcacgccgcg cgggcgcctc
    17101 gcaaccgacc gcgcattcga gtacttcggc gttccgcgac gcatgcgcgg cggcgcccag
    17161 aatacgctgt tttgaaagag gagagaaacc acaaaggcac tagggcataa atgcctcctt
    17221 ctccgtgtct ttatgcctcc gtggtgaaaa atgaagacgg tctatctctc actcggctcc
    17281 aacgtgggta accgcgaaga gatgcttcaa caggccgtcc gcctcctcgc ttccgccgat
    17341 ttccggatca cccggatgtc atccatctat gaaaccgagc cgcaagatgt ccgcggccaa
    17401 ccctggttct tgaacatggt attggaagcg gagacgacct tgttcccgag gcaactcctc
    17461 gggcggattc agaaagtcga gcagcagttg ggacggagac ggctcgcacc gaaaggtccg
    17521 cggaccatcg acatcgatat tctgctattc ggaactgcgg tgattgacac cgccggattg
    17581 acggtgcctc acccgcgcat ggcagagcgg cgcttcgttc tggagccgat gacggaactg
    17641 gagcccgatc tgcatcaccc gatcttgcgt cgcgcgatgc gcgaactgct tggcacagtc
    17701 cgcgaccaga cagtcagacg aaaaaccggc gactgatacc ggctctgtcc ggtcccgcct
    17761 acgggatgcg ttccgccagg gtcttcgcga gcgccacgcc gtctactcct tcggcgaggt
    17821 tggcgtatcc ggccaggtag ctgcccttgc ggaagacgca cacgcgacca aggtaccggc
    17881 tgttcacctg aaagccgccg tcggcaatgg cgactggctc gccctcgccg atccgggcct
    17941 tcactttttc tatcgatgct gctgccgcgt cgggtgaaga ctccgtcgcg atgaacgcct
    18001 tgccgaactc gtactcggct acgtatccgc gccgcagcgc ccgcattccc agtacgctct
    18061 gcggaaccag ccggaccgag tcctttacca gtttctcggc aggaaaccag ccgagcgccg
    18121 ccggaagttt cgtgctgccc tggatgcgct tttccatcgc ctcgacgaac tggcgcagcg
    18181 aagcggtgtg atccttgtca gggcttgcgg cgatctccac gaagtacttg tccttcgcaa
    18241 aaatcgcccg gcgtggctgg atctgtccgc ccatcccgat cgtctcagcg ggccgccgta
    18301 tgtcacggtt tgccgtgtag atcccatagg cgagctcagg gctgcccatc tcggagacgt
    18361 caaatacgaa cgtgttctca cccgacttgc acgtcactcc gtccatcttg tggaacgagt
    18421 agatgacgta cccctcggcg ttgccgttca tgtagtcgaa caggttgtcc gccgt
//
LOCUS       DATIFQ010000394         2346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2429537, whole genome shotgun sequence.
ACCESSION   DATIFQ010000394 DATIFQ010000000
VERSION     DATIFQ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2429537"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VLE22_19290"
     CDS             complement(<1..1082)
                     /locus_tag="VLE22_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16605.1"
                     /translation="MTFTDVVRFRCGVAHLAFVLTLCTAAQAQSKKTYVVVKPGTHPG
                     IIFSEEELPALRKRVLGTGLAARAYQRVKQNAMGSVVSALSVKQAVGREGDRLARQLE
                     SMALVYQIERDERLGRNAVELLKSVATGIDPWEFHKVVDSDFFATEHWPNAFAVAWDW
                     LYPIMTTEERVAILKSLEEWNAALYDHTESWWWRDAGYNCGAIPVGAQGTLLTAIQAE
                     SCHPEFQKWFSNCFRKIRQNYFPDTWRASGVCNEGPGYAHYHKNPTQFADAVRRTGGV
                     DIIGDSSAVNAMHYLRHQWMPQGGCGPVGDNTEYGRRVFQSIYLHGIRELGDRAGLWT
                     FENFTDFDRINPLHIVLYYPDGLEAVS"
     gene            complement(1142..>2346)
                     /locus_tag="VLE22_19295"
     CDS             complement(1142..>2346)
                     /locus_tag="VLE22_19295"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HSB16606.1"
                     /translation="LVMAADQFVVARGEEKTVIAGYHWFSDWGRDTMIALPGLTLVTG
                     RADVAKSILLAFAHHVDRGMLPNRFPDAGEAPEYNTVDATLWFFEAVRALLECKEDYE
                     FVSEHLYEVLADIIAWHEKGTRYGIRLDSDGLLLAGEPGAQLTWMDAKIGDWVVTPRH
                     GKAVEIQALWYNALRIMEHFAREFGDDTRRKRYGDIATAAHRSFEPTFWNEGTGCLYD
                     VVDGDKRDGSIRPNQVFAVSLPHSMVSPANARRLMDTVERHLLTPFGLRSLAPGDVQY
                     RGRYEGGPTSRDSAYHQGTVWPWLMGPFLTAYVKTHGGTSEARERAGRWLGALREYVR
                     EQGLGQLPEVFDGEAPQRAGGCIAQAWSVAELLRTTVEDVYDLQPAARGRLSAAALVA
                     GAAIRVRT"
BASE COUNT          428 a          762 c          694 g          462 t
ORIGIN      
        1 gacacggctt cgagcccgtc gggatagtag agaacgatgt gcagcgggtt gatccgatcg
       61 aaatcggtga aattctcgaa cgtccagagg cccgcacggt ctcccagctc gcggatcccg
      121 tggagataga tcgattggaa cacacggcgt ccgtactccg tgttgtcccc caccgggccg
      181 cagccgcctt gcggcatcca ctggtgccgc agatagtgca ttgcattcac ggcactgctg
      241 tctccgatga tgtccacgcc cccggtccgg cggactgcat cagcgaactg cgtcggattc
      301 ttgtggtagt gagcgtagcc cgggccctcg ttgcacacgc cgctcgcccg ccacgtatcc
      361 gggaagtagt tttgccgaat cttgcggaag cagtttgaaa accatttctg gaattccgga
      421 tggcagctct cggcctgaat ggcggtcagc agcgtcccct gggccccgac gggaatcgcc
      481 ccacaattgt agcccgcgtc gcgccaccac cagctctcag tatggtcgta aagggccgcg
      541 ttccattcct cgaggctttt cagaatggca acccgctctt ccgtggtcat gatcgggtat
      601 agccagtccc acgccaccgc gaacgcattg ggccagtgtt cggtggcgaa gaagtcgctg
      661 tcgacaacct tgtggaactc ccacgggtcg attcccgtgg cgacggattt gagcaattcg
      721 accgcgtttc ggcccaaccg ttcgtcacgc tcgatctggt agacaagcgc catcgactcg
      781 agttggcgcg cgagccggtc cccctcgcgg cccacggcct gtttcacgct taatgccgac
      841 acaacactgc ccatcgcgtt ttgtttcacg cgctggtacg ctcgggccgc caggcccgtt
      901 cccaacaccc gctttcggag cgccggcagc tcctcttcac tgaaaataat gccagggtgc
      961 gtcccgggtt tgacgacgac gtaagtcttc ttcgactgag cctgcgccgc tgtgcagagg
     1021 gtcaggacaa atgcaaggtg cgcgacaccg caccgaaatc ttacgacatc cgtgaaagtc
     1081 acgctcttta gaatagtgcg attggcccaa cctttcgtag cgtccagaaa ctcagtacca
     1141 atcaggttct gactcgaatg gccgcaccgg cgactagcgc cgccgcactc aggcgtccgc
     1201 gggccgcagg ctggagatcg tagacgtcct caacggtcgt gcgcagcagc tcggctacgc
     1261 tccaggcttg cgcgatacag ccgccagcgc gctgcggcgc ctcgccatcg aaaacctcgg
     1321 gaagttgtcc caaaccttgc tcgcgcacat attctcgcaa tgcgccgagc cagcggcccg
     1381 cccgttcgcg cgcttcactg gttccgccgt gagttttgac gtaggccgtg aggaacggcc
     1441 ccatcagcca gggccacacg gtgccctgat ggtaagcgct gtcgcggctg gttggaccgc
     1501 cttcgtagcg gccgcggtac tgcacatcac ccggggcgag gcttcgcaga ccgaacgggg
     1561 tgagcaggtg gcgctcaacc gtatccatca gccgcctggc gtttgcggga gacaccatcg
     1621 agtgcggcag gctgacggcg aaaacctgat tcggccggat cgagccgtct cgcttgtcgc
     1681 catcgacgac gtcgtaaagg cagccggtcc cttcgttcca gaacgtcggc tcgaaactac
     1741 ggtgcgccgc cgtggcgatg tctccgtacc gttttcgtct cgtatcgtcg ccgaactcgc
     1801 gcgcaaaatg ctccatgatg cgaagggcgt tgtaccagag ggcttggatc tcaacggcct
     1861 tgccgtgccg cggcgtcacg acccagtcac caattttggc gtccatccac gtcaactgag
     1921 cgcccggttc tccggccagg aggagtccgt cgctgtccag gcgtatcccg tagcgtgtac
     1981 ctttttcgtg ccaggcaatt atgtcggcta atacctcgta gagatgctcc gaaacgaact
     2041 cgtaatcttc cttgcattcc aggagcgccc gaaccgcctc gaagaaccag agggtcgcgt
     2101 ccacggtatt gtattcgggc gcctcgccgg cgtccggaaa ccgattgggg agcatgccgc
     2161 gatcgacgtg gtgcgcgaat gccagcagga tgcttttggc gacatcggcg cggcccgtga
     2221 ccaatgtgag tcccgggagc gcgatcattg tgtctcgtcc ccaatcgctg aaccagtgat
     2281 agccggcaat gaccgtcttc tcctcaccgc gtgcgacgac gaactgatcg gcggccatga
     2341 ccagcg
//
LOCUS       DATIFQ010000395         2028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1281606, whole genome shotgun sequence.
ACCESSION   DATIFQ010000395 DATIFQ010000000
VERSION     DATIFQ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281606"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VLE22_19300"
     CDS             <1..542
                     /locus_tag="VLE22_19300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB16607.1"
                     /translation="LGYMIGETDRLNRSVLQLLDFSRPVPEQTRDVDLSELLETMGQM
                     LERQYSGGQLRVATRIEPKLWLRDSSPEIIKQVVLNLTLNAVQAMGENGEIRLAAEGV
                     SGGKIVFAVTDQGPGIPEALHEKIFEPFFTTKQRGTGLGLAIVRKNVRHLGGEIVVES
                     PVEEGRGTRMVVTLPAGIK"
     gene            539..1750
                     /locus_tag="VLE22_19305"
     CDS             539..1750
                     /locus_tag="VLE22_19305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB16608.1"
                     /translation="MKPECFVFVGYLLLGLMSAPAQNSPFPPEQVARIQRGVHDLYSM
                     EYERAAENFQAMIRESPDDPTGYAYLARTIWIRELAGTQELSIDRFAASDFFEEQPKF
                     SPKVDPAVEDRFRKINAEAIARAKARLEKNPRDRAALFLRGLAYQNLSSFETSLKRSW
                     RKAMGSGRNAAGDHARLLRIDPSFHDAKLSLGAKDHVLGSLPLGWKLILGVIGYTGDR
                     ERGRQTLEFVARKGTLLADDARILLILIHTRQKDYQKAYDYLAELQQKYQQNYLIRLD
                     MGGMELLMNRPERAIAIYGEVLRRRNTGERNYSGLELAFLCNRLGVAFRQAGDLEGAV
                     GWFRKALAENGRSSRSTTVARLELGKTFDLMGRRAEAVEEYQAVLAAEDVAGSKLEAG
                     SLLKISFRPRR"
     gene            complement(1751..>2028)
                     /locus_tag="VLE22_19310"
     CDS             complement(1751..>2028)
                     /locus_tag="VLE22_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16609.1"
                     /translation="FECKDGRLEFEDRSAKFGFAQPQKLSVQWFRFDNATGNKSPLAS
                     EAGFTIPTDQAEYLAAEIRGSEPKKSVTVYLRREAAARRVVGIERFW"
BASE COUNT          424 a          585 c          633 g          386 t
ORIGIN      
        1 acctcggcta tatgatagga gagacggacc ggctcaaccg ttctgtcctc cagttgctgg
       61 acttctcacg gccggttccc gaacaaaccc gggacgtcga cctttccgaa ttgttagaaa
      121 ccatgggcca gatgctggag cgacagtact cgggcggcca gttgcgtgtc gcgacaagga
      181 tagagcccaa gttgtggtta cgggacagca gtcctgagat tatcaagcag gtggtcctaa
      241 acctcaccct gaatgcggta caagcaatgg gggagaacgg cgagattcgt ctcgcggcgg
      301 aaggtgtgtc cggcggaaag atcgttttcg ctgtgaccga ccaggggcct ggaatccccg
      361 aagcgctgca cgagaagatc ttcgaaccgt tcttcaccac caagcagcga ggaacgggac
      421 tcggcctcgc gattgtccgg aagaacgtcc gccatctggg cggtgagatc gtcgtcgaaa
      481 gtccggttga ggaggggagg ggtacgagga tggtagtgac cctgccggcg ggaatcaagt
      541 gaaaccggag tgcttcgtct tcgtgggcta tcttttattg ggcttgatgt ctgctccggc
      601 tcagaacagc cccttccccc cggaacaggt agcgcgaatc cagcggggcg tccacgatct
      661 ttacagcatg gagtacgagc gtgccgcgga gaatttccag gcaatgattc gggagtctcc
      721 ggacgatccc acaggatacg cctacctcgc gcgcaccatc tggatcaggg aactcgccgg
      781 cacgcaggaa ctcagcatcg accgctttgc tgcttcggat ttcttcgaag agcagccgaa
      841 attcagcccc aaagtcgatc cagccgtcga ggatcggttc cgcaagatca acgccgaggc
      901 gatcgcgaga gcgaaagcac gactcgaaaa gaacccgcgc gaccgcgccg cactatttct
      961 ccgcgggctt gcctaccaga atctctccag ttttgagacc tctctcaagc gcagttggcg
     1021 caaggcgatg ggctccggaa ggaacgccgc cggcgaccac gcccggttgc tccgtatcga
     1081 tccgtccttc cacgatgcaa agctgtccct cggcgcgaaa gaccatgttt tgggcagcct
     1141 tccgctcggg tggaagttga tcctcggcgt gatcggatat accggtgaca gggagcgtgg
     1201 ccggcagaca cttgagttcg tggcgcggaa gggcaccctg ctggccgacg atgcgcgcat
     1261 cctgttgatt ctgattcaca cgcgacagaa ggactaccag aaggcttacg attacctggc
     1321 ggagcttcag cagaagtacc agcagaatta cctgataagg ctggacatgg gcggcatgga
     1381 actgttgatg aaccggccag agcgtgcgat cgcaatctac ggagaggtcc ttagacggcg
     1441 aaacacggga gaacggaact actccggcct ggagctggcg ttcctctgca atcggctggg
     1501 cgtggcattc cggcaggccg gcgatctgga gggcgccgtc ggatggttcc gcaaggccct
     1561 tgctgagaac gggcgttcca gccggtcgac cactgtggcg cggctcgaac tgggaaagac
     1621 gttcgacctt atggggaggc gagcggaagc tgtggaagag taccaggcgg tcctcgccgc
     1681 ggaggacgtg gcggggtcga aactggaagc ggggtcgctt ctgaaaatat ccttccggcc
     1741 gcgcagatga ttaccagaat cgctcgatac ccacgacccg gcgcgcagcc gcctcccggc
     1801 gaagatacac agtgacgctc ttcttcggct cggagccccg aatctccgcg gcgaggtatt
     1861 ccgcctgatc tgttgggatc gtgaagcccg cttcgctcgc aagcggagat ttgttcccgg
     1921 tcgcgttgtc gaatcggaac cattgaaccg atagtttctg tggctgagcg aatccgaact
     1981 tcgctgaccg gtcctcaaac tccagccttc cgtccttaca ttcgaacc
//
LOCUS       DATIFQ010000396         3865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1281659, whole genome shotgun sequence.
ACCESSION   DATIFQ010000396 DATIFQ010000000
VERSION     DATIFQ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281659"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VLE22_19315"
     CDS             complement(<1..363)
                     /locus_tag="VLE22_19315"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSB16610.1"
                     /translation="MLRPMYRYVEVSHPIEAGMKTYPGLPEPSVEVLLDYGPSRERYQ
                     GKAEFLIASLHLCGNTGTYVDSPRHRYRDGADLAAIPLEAVAHVPVTKLDARALPARG
                     VAAEVFKGVEIAGRAVLIN"
     gene            472..2589
                     /locus_tag="VLE22_19320"
     CDS             472..2589
                     /locus_tag="VLE22_19320"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HSB16611.1"
                     /translation="MAARTARYFFACLLALAAFQSAPPVFSQASLPVPDKYIERQKLL
                     DEIAWPSHEPLMFLRRHGGMDVDAESYYFRYHTEENVRKIAATGLEMSRILHFYKGFG
                     FETEQEEMQRTVELARLFHKYNMKVPVYIGGTMFSETFFLETPEARKWVRVDQWGQPV
                     TYGSHQTARYFACLNNPGYVEYLKKVLKKATVEVKADRVFFDNFTLYAEPQSCHNEAC
                     VREFRRYLSRKYDADARVRRFGFASVEGIHPPIWNVLNEPWQLETISDPLLQEWIDFR
                     CWTISNYYRQLYDYMKSLNPGASVGVNIKGVMGRNRAFRDGIDHPRFADVGDWFELDP
                     GFAAGISENGSLVSEIRSYKIGQSLGVPFDFEAETELRLAEYMAFNFQKETLGFGTNG
                     GFREFLWVPHLFRYFDFFKENDQRFYRGAQSVGDVAVLRGYASMANNNDSAHRATVLA
                     EQLLIQGKIPFHIIFDRHLPELSKYKVLMLTEQESLSDSDIEKIKGFVERGGGLVATG
                     AAGDYDQWRRPRLDKTLAAALGFRKGKVTRGTFGKGRFVYLPAISPATDSSAAGNRAR
                     NLSIGDSFTARGYRDFTPEQWLLPKNRLEILDALRWAAAGRFSAEIQAPLTTVAELTQ
                     KASDNVVMLHLLNYDERTAVPDVQVDLAVPRGRRVKSVTLLSPDADGTKQLEYTASNG
                     RVRFKVPKLAKYSLAVVGLTSEQ"
     gene            2608..>3865
                     /locus_tag="VLE22_19325"
     CDS             2608..>3865
                     /locus_tag="VLE22_19325"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HSB16612.1"
                     /translation="MMLHGFLLLAAAIAGTGLAAAPAPILEVSRNGHYLTKDGKPFFW
                     MGDTAWSLINLYAADEAEAYLEHRRGQGFNVVHVMLLFDGGPGLETAAADQTRHLPFV
                     DMNPATPNEAYFRNVDRLVETARRKGLILAVMPCGGSSGSFVKKKKIITKETARAYGA
                     WLGRRYKDSPNIVWANGFDLRPWEYEDIAREFAAGLGEGDGGSHLISYHPSGGASSSY
                     FHNEPWLAMNFIQTWADFTKIYSMVHADYLRQPAKPVIHAEGAYEEGPEYPTRPITPL
                     VVRRQAYWAVLAGGFHTYGHNDMWRKNPTWRRSLDAPGAQAMGVLKTIFTSREWWKLV
                     PDQSVFSTGAGEGKTLNAAARSEDGGTVIVYLSSPAGVTINMSRLTAGRRSRATWVNP
                     VTGKGTNGGEFPNKGDRRFVPPESRED"
BASE COUNT          786 a         1126 c         1218 g          735 t
ORIGIN      
        1 attgatgagg actgctcttc cagcgatctc cacacccttg aagacttccg cggcaacacc
       61 tcgggcaggc agagcacgcg catcgagttt cgtcaccggc acatgggcga ccgcttccag
      121 tggaatcgcg gccagatcgg ccccgtcgcg atatcggtgg cgaggggaat cgacgtaggt
      181 gcccgtatta ccgcagaggt gaagcgacgc tatcaggaac tctgcttttc cctggtatcg
      241 ctcccgcgaa gggccatagt ccaggaggac ttccacactc ggttcgggca ggccgggata
      301 ggtcttcatc cctgcttcta tggggtggct tacctcgacg tacctgtaca ttggtcgaag
      361 catatcagtt cgagaggctt ccactgaatt tctcgtgatc gactcgctgg ccacggaaga
      421 cggtggcctg cgtcactgac gaccgcggtt cctacagtag actctggagg tatggccgcc
      481 cgaactgcga ggtacttttt cgcctgtctg ttggcgctgg ctgcattcca gagtgcgcct
      541 ccggtctttt ctcaagcaag cctcccggtg cccgacaagt acattgagcg ccagaagctc
      601 ttggatgaga tcgcctggcc aagtcatgag ccattgatgt ttctccgccg gcatggcggc
      661 atggacgtcg atgccgagag ctactatttc cgttaccaca cagaagagaa tgtccggaag
      721 atagccgcca ccgggctcga gatgtcgcgc atcctgcatt tctacaaggg attcggtttc
      781 gagaccgaac aggaggagat gcagcgcacg gtggagctgg cccggctatt tcacaagtac
      841 aacatgaagg tcccggtcta tataggcggc acgatgttct cggagacctt cttcctcgaa
      901 acaccggagg cgaggaagtg ggtgagggtc gaccagtggg gacagcccgt tacgtacggc
      961 agccatcaga ctgcgcgtta cttcgcctgc ctcaacaacc ccggctatgt tgagtacctg
     1021 aaaaaagtat tgaagaaggc gaccgtggaa gtgaaagccg accgcgtttt cttcgacaac
     1081 ttcacactgt atgccgaacc gcaatcgtgc cacaacgaag cctgcgtccg ggagtttcgc
     1141 cgctacctgt cccgcaagta tgacgcggac gcccgcgtcc ggcgcttcgg cttcgcttcg
     1201 gtcgaaggca tccatcctcc catctggaac gtcttgaacg agccctggca actggaaacc
     1261 atcagcgatc cgttgttgca ggagtggatc gacttccgct gctggacgat cagcaactac
     1321 taccgccagc tctacgacta catgaagagc ttgaatcccg gagcttcggt cggcgtgaac
     1381 atcaaggggg ttatgggccg caaccgcgcc ttccgcgacg gtatagacca tccacgcttc
     1441 gccgacgtcg gagactggtt cgagttggac ccgggattcg ccgccggcat cagtgagaac
     1501 ggctccctgg tcagtgagat ccgcagttac aagatcggac agagcctggg cgttcctttc
     1561 gattttgagg ccgagacgga actccgcctg gccgagtaca tggcgttcaa ctttcagaaa
     1621 gagaccctgg gcttcggaac caacggcgga ttccgggagt tcctctgggt cccacacctg
     1681 tttcgctatt tcgatttctt taaggaaaac gaccagcggt tctaccgggg ggcacaaagc
     1741 gtgggcgacg tagctgttct gcgaggctac gcctccatgg cgaacaacaa cgattccgca
     1801 catcgcgcca cggttttggc cgaacagttg ttgatccagg gcaagattcc atttcacatc
     1861 atctttgacc ggcaccttcc cgagctctcc aagtacaaag tgcttatgct cacggagcag
     1921 gagagcctca gtgacagcga catcgagaag attaaaggtt ttgttgagcg cggcggagga
     1981 ctggtggcga caggcgcggc gggggattac gatcagtggc gcagaccgcg cctggacaaa
     2041 accctagcgg cagcgctggg atttcgtaag ggaaaggtga cgcggggcac tttcggcaag
     2101 gggcgcttcg tttacctgcc tgccatcagc ccggccacgg attcgtccgc tgccggcaac
     2161 cgcgcccgaa acctcagcat aggcgactcg ttcacggcga gaggctaccg cgactttacg
     2221 ccggagcagt ggctccttcc gaagaaccgg ctggagatcc tggatgccct ccgctgggcg
     2281 gcggccggac ggttctccgc cgagatccag gcgccgctga ccaccgttgc cgaacttacg
     2341 cagaaggcgt ccgacaacgt ggtgatgctc catctgctca attacgacga gcggacggcc
     2401 gtgccggacg ttcaggttga tctggcagtt cctcgaggcc ggagggtgaa gtccgtcacg
     2461 ctgctctccc cggatgcgga cgggacgaag cagttggaat ataccgcctc caacggacgg
     2521 gttcgcttca aggttccgaa gctggccaag tacagcctcg ctgtcgttgg actgacctcg
     2581 gagcagtaag gatatcgagt gataaagatg atgctacacg ggttccttct tctggcggct
     2641 gcgatcgcgg ggacaggcct ggcagcggcg ccagcaccga ttcttgaggt cagccgaaat
     2701 ggccactacc tgacgaagga cggcaagcct tttttctgga tgggagacac ggcctggtcg
     2761 ctgatcaacc tctacgccgc cgatgaggcc gaggcgtatc tggagcaccg ccgggggcag
     2821 ggattcaacg tggtccatgt gatgctgctg ttcgacggcg ggccggggct ggagaccgct
     2881 gccgcggacc agacgcgcca cctgcccttt gtggacatga acccggccac tccgaatgag
     2941 gcgtatttcc ggaacgtgga tcgcctggtg gagactgcgc gccggaaggg cctgatcctc
     3001 gccgtgatgc cgtgcggcgg ttcgagcggg tccttcgtca agaagaagaa gatcatcaca
     3061 aaggagaccg cgagagcgta cggggcatgg ctgggccggc gctacaagga cagcccgaac
     3121 atcgtctggg ccaacggatt tgatctgcgc ccctgggaat acgaggatat cgcccgcgaa
     3181 ttcgccgcgg gcctcgggga aggggacggc ggctcccacc tgatttcgta ccatccaagc
     3241 ggcggcgcgt catcgagcta cttccacaac gagccctggc tggcgatgaa cttcattcag
     3301 acgtgggcgg acttcacgaa aatctactcg atggtccacg cggattacct ccgtcagccg
     3361 gccaagccgg taattcacgc cgagggggcc tacgaggaag gcccggagta cccgacaaga
     3421 ccgatcacgc cgctggtggt aaggcggcag gcgtactggg cagtgctcgc gggcggcttc
     3481 cacacgtacg gtcataacga catgtggcga aagaatccga cgtggcgccg gagtctcgat
     3541 gcgcccggag cacaggcgat gggtgtattg aagacgatct tcacatcgcg ggaatggtgg
     3601 aagctcgttc cggaccagtc ggtgttttcg accggcgcgg gcgaagggaa gaccttgaac
     3661 gcggccgcgc gctcggaaga cggcggaacg gtcattgtgt atctgagcag ccccgccggg
     3721 gtaacgatca acatgagcag gctcacggcc gggcgtcgta gccgcgcgac gtgggtgaat
     3781 cccgtgacgg ggaagggtac gaacggcggg gagttcccga acaagggtga ccgccgattc
     3841 gttcctccgg agtcacggga ggatg
//
LOCUS       DATIFQ010000397        15615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2237799, whole genome shotgun sequence.
ACCESSION   DATIFQ010000397 DATIFQ010000000
VERSION     DATIFQ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15615
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237799"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VLE22_19330"
     CDS             complement(<1..489)
                     /locus_tag="VLE22_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16613.1"
                     /translation="MQAHKITAARVGIFGIGLAAYWPQFPGLKERLEGYQAEVEARCA
                     SFGARLVSAGLVDTPERARRAGEQFARERVDLLLCYVGTYATSSQVLPVIQQARAPVV
                     VLNLQPVPALDYENTDTGEWLANCSACCVPEISNALARSSIPFHVVSGVLFEDDAAWR
                     EIA"
     gene            complement(529..2919)
                     /locus_tag="VLE22_19335"
     CDS             complement(529..2919)
                     /locus_tag="VLE22_19335"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HSB16614.1"
                     /translation="MTKRHRLPLALAAVAPLLFFVCFGDQDPGGPLYRSGTASVEERV
                     EDLLRRMTLEEKVAQMTVRSSTHMLEDGRLPLKDGQVDESKIEAVLKGRCYGLLEGSF
                     GGDIRIMPAIINAAQRYCLEKTRLGIPMIPYIETLHGVMFYGATIYPQSIGMASTWNT
                     NLVHKAATEIAAEAASVGIRQALAPTVDVIRDQRWGRVEEAYGEDPYLVSRLSVAYIL
                     GLQGPREESVKGIAGNRIGAMCKVFIGHGFPERGINLAPILMPDLEMRNFCLPPFEAS
                     IREANVYSVMPGYNEYNGIPLHGHVELLEGVLRREIGFRGYIFSDYGGVEMMSGFHKI
                     AGSLAEAATMGVRAGVDVEAPSDSCYRSLPALVREGKVAIDLIDASVRRILEAKFRLG
                     LFEHPYVEPEAYKQHVRAPSHIATAREMAEESITLLQNRGPLLPLDEKALKSIAVIGP
                     NADQVQFGDYSATKDNRDGVTILQGIRALLPKTVGVRYARGCTLLGSDRSGFPEAVEA
                     AGASDVAVVVVGETSMPLQGVGWLKEPVDFRHATSGEGYDRTNLSLTGVQEDLIKAVA
                     AAGKPVVVVLVNGRPQAIPWVKENIPAILEAWYPGEQGGHAVASILFGRVNPSGRLPI
                     TIPSTTGHLPVHYNRKPSASGFYRRPGSPEKPGRDYVDASPEPLFPFGHGLSYTSFEY
                     SDLRLSATSIPPGGVIEARFRVRNTGRRDGKEVVQLYLRTNSRLITMPVMELRKFDKI
                     SLAAGEEREVTLKLGSADLAHLKSNFESIVEECGYEVMVGASSADIRLRGGFEVRR"
     gene            complement(2978..5227)
                     /locus_tag="VLE22_19340"
     CDS             complement(2978..5227)
                     /locus_tag="VLE22_19340"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HSB16615.1"
                     /translation="MKPHEYTVVGTRPVRHDGYDKVTGRALYGADFQAAGMLHGFVVR
                     SPHAHARIRRIDTSKAESFPGVRAVVTAADFPQGPDRMVDLGDGPVPLSYVRGNLLAS
                     GKALYRGHAIAAVAAATPHAAEEAAGLIKIDYDPLPCVLTAPEAMREGAPVLLGDLKT
                     KEFGEDTGKVSNVAEHFRWTLGDGEQGFAAADVIVEREFHTSTVHQGYIEPHNAAALW
                     NNDGRVSVWCSTQGAFNVRDVLSHVLDLPVSRIKVTPMEIGGGFGGKINVYLEPVAAL
                     LSKKAGRPVKIVMSRRDVFEGSGPTPGSYVRVKIGAQKDGRIIAAQAYMAYEAGAYPG
                     SMVLPGAMCIFAAYDIPNVVIDGFDVVVNKPSTAAYRAPGATNAAFAAESVVDELAEK
                     LAMDPIEFRLRNAAKEGTRRADGPKYQRIGCVEVLEAMKQHPHYSAPLEGPNRGRGVA
                     VGFWFNVGFPSSCTIGVNADGTVSLVEGSTDIGGTRTSVAMQAAEVLGIPAEDVLPSV
                     VDTDSVGYTAVTGGSRTTFATGWAAIEAARDVLRQMKERAAMLWETEAVTVEAERGVF
                     RSGDRSMTFRELAGRVAETGGPVVGRGAVDPRGVGGSFAGCVADVEVDPETGKVTILR
                     FTSVQDAGKAIHPSYVEGQMQGGSVQGIGWALNEEYRMSEGGAVLNSSFLDYRMPTSL
                     DVPMIDTVIVEVPNPGHPFGVRGVGEANIVPPPGAIANAIYRATGVRMRQLPMNPQTV
                     MEGIWSWQP"
     gene            complement(5224..5712)
                     /locus_tag="VLE22_19345"
     CDS             complement(5224..5712)
                     /locus_tag="VLE22_19345"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSB16616.1"
                     /translation="MTHRKVHVRTTVNGEAVEFLCEPRQSLLECLRDIVGLTGTKEGC
                     NDGNCGACTVILDGDIVNSCLVLGVEVEGRNITTIEGIARSGELHPVQKAFLESAALQ
                     CGFCTPGLIVATKALLDRNPEPDEESIRQWLANNLCRCTGYDKVVRAVEQAAADLREV
                     KG"
     gene            complement(5709..6581)
                     /locus_tag="VLE22_19350"
     CDS             complement(5709..6581)
                     /locus_tag="VLE22_19350"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HSB16617.1"
                     /translation="MKWFDYTAPVTLAEAMNAVASGPRAKALAGGTDLLVQMRAGRVE
                     TDLVVDIKRVPELNELSYDSARGLTLGAAAPCCRISENASVRRHYPALAEVAALIGGV
                     QIQSRASLGGNLCNAAPSADAVPMLIAYGAICCIAGANGTRQVRVEDFCTGPGRTVLE
                     PGELLVSFQLPPPPAGSGARYLRFIPRNEMDIAVAGAGVQVVLDDGHFRTARIALAAV
                     APTPLFVPEAGESLAGKPAVPESIHAAAEIARAAAQPITDMRGTEEYRRHLCAVLTRR
                     ALEAAVQRATGGRI"
     gene            complement(6650..7183)
                     /gene="rpiB"
                     /locus_tag="VLE22_19355"
     CDS             complement(6650..7183)
                     /gene="rpiB"
                     /locus_tag="VLE22_19355"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HSB16618.1"
                     /translation="MDSSSGGIGRRVFLGSGVAGAVAAAPGVRAMRLAIGSDHAGFPL
                     KGPVIEMLRSWGHSVKDLGCFSTQPVDFPDIARQVCAEIKGGRADRGIMVCGTGIGAA
                     IACNKIRGIRAALCHDTYSAHQCVEHDDVNVLCVGNWIIGIKVAEEILHAFLNAKFST
                     SEEFRRRVRKLEEMEKL"
     gene            complement(7386..7472)
                     /locus_tag="VLE22_19360"
     tRNA            complement(7386..7472)
                     /locus_tag="VLE22_19360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7436..7438),aa:Leu,seq:gag)
     gene            complement(7520..7954)
                     /gene="secG"
                     /locus_tag="VLE22_19365"
     CDS             complement(7520..7954)
                     /gene="secG"
                     /locus_tag="VLE22_19365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSB16619.1"
                     /translation="MVVLLTIIHVIVCLFLIIVVLLQSGKAADLAGAFGGMGSQTAFG
                     PRGSATVLSKATTTAAVLFMVTSISLAILATRTAGVGSTVLEGHTPGKTTTPSTPSIP
                     APQGGVGTIPQGGKSQGIPIQLPRPTQTAPAKPAPAKPAPQK"
     gene            complement(8073..9242)
                     /locus_tag="VLE22_19370"
     CDS             complement(8073..9242)
                     /locus_tag="VLE22_19370"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSB16620.1"
                     /translation="MEPFRGVDYLHIDSLFNGDELLVRQTARQFVDDRITPVIRDCYR
                     EGRFPMELIPEMAKLGFFGANLEGYGCPGMSNVEYGLIMQELERGDSGVRSCVSVQGA
                     LVMYPIHAFGSEEQKRKWLPRLQSGEVLGCFGLTEPDFGSNPGGMRATARRDGDAWVL
                     NGEKSWITNGSVADLAVVWARTEEGVRGFLVERGTAGYTTSDIHGKWSMRASVTSSLS
                     FSDCRIPAENVLPGAKGLKTALMCLTQARHGIGWGVIGAAMDCYETARRYTLIRKQFD
                     DRPIASHQLVQEKLVWMITEITKAQLLAVQVGRLKDRGKAEPAHISMLKRNNAAIALE
                     CARLGRDLLGANGITDEYPIMRHLCNLETVKTYEGTDHIHALVIGEQVTGIPAYR"
     gene            9313..11175
                     /locus_tag="VLE22_19375"
     CDS             9313..11175
                     /locus_tag="VLE22_19375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB16621.1"
                     /translation="MLRPILLLPLSVLALCGQIPKDPSARLLDEAYKALRAKHYDSAI
                     ESFRKAIDLAPDRAAVRKDLAYTYLKIGETEAARGQFSEAMRLAPGDHHAALEFAFLC
                     YETGKTVEARRVFDRVRMQGDPASRETAGRAFQNVDRSLAEGIEQWTRALALHPDDFS
                     AHHELARLAAQRDDWKLAAAHFERAWQLRPDLRSVLVELGRARRELGEEERAMAALLA
                     ASRGAEPRAAEDARSLMPARYPYVDEFRQALSLDPKNVELRRELAYLLLEMGKAEEAK
                     AEFAALPAAAPAEPPGKASIAPKEMARRSYEKGYLQDALTYYSAAQEADPLDFGVMLQ
                     LGWTNNLLRRDRDAIRWFALARRSPEPAIAAEAAKAERNLKPRLTRFRTSAWIFPSYS
                     SRWRDVFTYGQLKADFKLSALPLRAYASIRFIGDTRTAMGDAPPQYLSESSVILGVGL
                     ATDYWHGLMLWGEAGSAINYLGNRRGAPRMAPDYRGGLAFYRGFGRLIGTPARGMFFE
                     TNGDGVFVSRFQNDVVLYSQNRFGLTLPAANSLGGLEAQLYWNGNASTDLRRQYWANV
                     VETGPGLRFRWKGLSPSWLFSVNFLRGAHTRNVGNPRSPNFFDLRVGIWYSVTR"
     gene            11387..13195
                     /locus_tag="VLE22_19380"
     CDS             11387..13195
                     /locus_tag="VLE22_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16622.1"
                     /translation="MERVKRGAYLILEGDSPLAGELGFRPGPGRVHVSSVEDLRRPGL
                     SIVWESAVDIPIFEMPQDARVFARERWRKAPLLAGFRRGQGAVLWLAVSPGQRGYERF
                     PYLVQALADLGLEPPFRSRGLWAFFDSSYRARADLEYLARRWRAAGISALQVAAWHYF
                     EPDESRDHYLRTLVESCHRQGILVYAWLELPHVSDRFWNDHPEWREQTALLQDARLDW
                     RRLMNLANRDCFRAAATGVRHLLGRFDWDGVNLAELYFESLQGHATPSRFTPMNADVR
                     REFRDRAGFDPLELFNPASARHYSRNESDLRDFLGYRVDLVRRIQTEWIGEVESMRQS
                     KPDLGLVLTHVDDGLDPGIRDAIGADTAALLPLLARHSLTFLIEDPATVWNFGPDRYR
                     QIALRYARITPHTSRLGMDINIVERYQDVYPTKQQTGGELFQLVNTASRVFPRVALYF
                     ENSILAPDIPWLASAAATVDRFECAGTRVAVQSPRGAGVPWKGPALVDGRPWPAYDGE
                     TVWLPAGSHAIEPAEGEPHARLLDFNGDLVAARYLTDGLEFSYRSDAPAFAFLDRRPR
                     AVEVDGEVWKIRGVDSEGRHMIRLPRGQHLVTVKTR"
     gene            complement(13201..14544)
                     /locus_tag="VLE22_19385"
     CDS             complement(13201..14544)
                     /locus_tag="VLE22_19385"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB16623.1"
                     /translation="MSAIGNPSPSPAGTPVQEGPKPSPAPPKAPKPWSLWFILAVLVS
                     IAVAMYWQLSRPQQTASTAAAIRTATVARGAVERVVRLSGQTAARNFASITVPLMRGP
                     DSGRELILVELAKSGSFVKKGQLVAQLDTQAARDHIDDVNSIVIQAETDIRKRRAEQA
                     IDWENLQQNVRVAKAELDQARLDNSASAVRTVVDQELLRLFVEESEARYKQLQQDLEK
                     TRAVHRAELRILEITRRRHEMHRQRHLIDLKRLTFIAPIDGLAVMSTFFRAGEMSQIQ
                     LGDQVYPGQTFMKIVDVSSMQVEADANQTESNDLRVGQRASVTLDAFPGVAFPGRVFS
                     IGAMSVRGWRENYYIRRVPVRVTIEGADPRLIPDLSAAATVTVGHQENTLIAPLEAVH
                     TEGGKSAIFVKDGDRFDKREVQLGLRSFTHVAVLSGMKAGEVVALGPPATVVAQR"
     gene            14862..>15615
                     /locus_tag="VLE22_19390"
     CDS             14862..>15615
                     /locus_tag="VLE22_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16624.1"
                     /translation="MLRGKFLFFGLMVCLVSAAWGGTFGKVVPIGGHASDIALDERRG
                     VVYLANFTANRIEVISISDYSRKTPIRVPRQPGSIALSPDARYMVVTHFGKWDATFTI
                     KPSVTILDLDTNQSRNIDLGDNPLTVAFGGGSRALLVTEGTVAAAGRFLLLDPASGSL
                     EVLAPALVGADQLPVPFATFPPEIIKASGGVSGDNRWIIGMASAQQAEQALTVQYRYN
                     VQTRQLSVIFITSAPPLGPRVVSVNQDASMSLL"
BASE COUNT         2800 a         5125 c         4708 g         2982 t
ORIGIN      
        1 ggcgatctcc cgccacgccg catcgtcttc gaacagcacg ccggaaacga catggaacgg
       61 aattgaggaa cgtgcaagcg cgttcgagat ctccggaacg cagcaggccg agcagttcgc
      121 aagccactcg ccggtgtcgg tgttctcgta atcgagcgcc ggaaccggct ggagattgag
      181 cacgacgacc ggcgcacgcg cctgctggat caccggcagg acttgtgaag aagtggcata
      241 cgttccgacg tagcagagca atagatcaac ccgctcgcgc gcaaactgct cgccggcacg
      301 acgcgcgcgc tccggtgtgt caacaaggcc ggcggaaaca agccgagcgc cgaacgaagc
      361 gcagcgagcc tcgacttccg cctggtagcc ctccagccgc tccttcaggc cgggaaactg
      421 cggccagtac gccgccagac cgataccaaa gattccgacc cgtgcagccg tgattttgtg
      481 tgcctgcatc ttccctcctc cgttcccgtt acattagggt ccacggcctc atctcctcac
      541 ctcgaagccc cctcgcaggc ggatgtccgc gctcgacgcg ccgaccatga cttcataccc
      601 gcattcctcg acaatgctct cgaagtttga cttgaggtgg gcgaggtcgg ccgagcccag
      661 cttcagcgtg acttcgcgtt cctctccagc cgcgaggctg atcttgtcga atttcctcag
      721 ttccatcacc ggcatcgtga tcaggcgcga gtttgtgcgg agatagagct gaaccacttc
      781 cttaccgtcc cttctgccgg tgtttctcac ccggaaccga gcctcgatca ctccaccggg
      841 cgggatgctg gttgccgaca gacgaaggtc ggagtactcg aacgaggtgt agcttaaccc
      901 gtggccgaat ggaaagagcg gttccggcga ggcatccacg taatcccggc ccggcttctc
      961 cgggctgccg ggccgcctgt agaagccgga cgccgatggc ttgcggttgt agtgaaccgg
     1021 caggtgaccc gtagtgctcg gaatcgtaat cggcagcctg ccggacggat taacccggcc
     1081 gaacagaatc gacgcaaccg cgtgaccgcc ctgttcaccc gggtaccacg cctcgagaat
     1141 tgccgggatg ttctccttca cccacgggat cgcttgcgga cgtccgttca ccaaaaccac
     1201 cacgaccggc ttgcccgccg ccgccacagc cttgatcagg tcttcctgaa ccccggtcag
     1261 gctgagattt gtacggtcgt atccttcacc ggacgtggcg tgacggaaat ccacaggctc
     1321 tttgagccag ccgacacctt ggagcggcat gctcgtctcg ccaaccacta cgaccgctac
     1381 gtcactcgcg cccgcagcct ccaccgcctc cggaaatccg gagcggtcac ttccaagtag
     1441 ggtgcaccca cgcgcatagc gcacgccgac cgtcttcggg agcagagctc gaatgccttg
     1501 caggatcgtg acaccgtcgc ggttgtcctt cgtggcagaa tagtctccga attgcacctg
     1561 gtccgcgttc gggccgatga ccgcgatcga cttcagtgcc ttctcgtcga gcgggagcag
     1621 cgggccacgg ttctggagca gagtgatcga ttcctccgcc atctcgcgcg cggtggcaat
     1681 gtgcgagggt gcgcggacat gctgtttgta cgcttccggt tcgacgtacg gatgttcgaa
     1741 caatccaagc ctgaacttcg cctccaggat gcggcggacc gaggcgtcga tcaggtcaat
     1801 cgccaccttg ccctcgcgga cgagcgccgg caatgagcga tagcacgaat ccgacggcgc
     1861 ttccacgtcc acaccggcgc ggactcccat cgtcgcggcc tcagcgagac tccccgcgat
     1921 cttatggaag cccgacatca tctcaactcc gccatagtct gagaagatgt acccccgaaa
     1981 gccaatctcg cgacgcaaca ctccttccag aagctcgaca tgaccgtgaa gcggaatgcc
     2041 gttgtactcg ttgtagccgg gcattacaga gtacacgttc gcctcgcgga tggaggcctc
     2101 gaacggcggc aggcagaaat tgcgcatctc taagtcaggc atcaggatcg gtgcgaggtt
     2161 gatgcctctc tccggaaagc catggccgat aaagaccttg cacatcgcgc cgatacggtt
     2221 tcccgcgatg cctttcacgc tctcttctcg cggtccttga agtcccagga tgtaggcgac
     2281 ggaaagccgg ctgacgaggt agggatcttc accgtacgcc tcttcgacac ggccccagcg
     2341 ttggtcccgg atcacgtcca cggtcggagc gagagcctgg cggatcccca ccgaggcggc
     2401 ctccgcggcg atttccgtcg cggctttgtg cacgagattg gtgttccacg tgctggccat
     2461 gccgatcgac tgcggataaa tcgtcgcgcc atagaacatt acgccgtgaa gcgtctcgat
     2521 gtaggggatc attgggatac ccagacgagt tttctcgagg cagtagcgct gcgcggcatt
     2581 gatgatggcg ggcatgatgc gaatgtcacc gccgaaggat ccctccagca gcccatagca
     2641 cctgcccttc aggacagctt cgatcttgga ttcatcgacc tgtccgtcct tgaggggcag
     2701 ccggccatcc tccagcatgt gggtggagct tcggacggtc atctgcgcga ccttttcttc
     2761 gagcgtcatc cgccggagaa ggtcttcgac tcgctcctca acagaagcgg tgccgctccg
     2821 gtagagcggc cctccgggat cctgatcgcc gaagcagaca aaaaacagta gcggcgcgac
     2881 cgccgcgaga gccaggggga gacgatgtcg ttttgtcacc gctcccaatg atattgcaga
     2941 ccgaggccgg tctcgcgctc cggccttacc cgcgcggtta cggctgccag ctccaaatgc
     3001 cttccatcac agtctgcgga ttcatgggaa gctggcgcat ccgcacgccc gtcgcccggt
     3061 agatggcgtt cgcgatggcg cccggaggcg gaacgatgtt cgcttcgccc accccacgaa
     3121 ctccgaaagg atgcccgggg ttcggcactt cgacgatcac ggtatcgatc atcggcacgt
     3181 caaggcttgt tggcatccgg tagtcgagga aactcgagtt gagcaccgca ccgccctcgc
     3241 tcattcggta ctcctcgttg agagcccagc cgatgccctg gacgctgcct ccctgcatct
     3301 gtccctcgac ataactcgga tggatcgcct tgcccgcgtc ctgcaccgac gtgaaacgca
     3361 gaatcgtgac cttgcccgtc tctggatcta cttcgacatc ggcgacacag ccggcaaacg
     3421 atccgccgac gccgcgcggg tccactgcac cacggccgac gacggggccg ccggtttctg
     3481 ccactcgccc ggccagttcg cggaacgtca tcgagcggtc accggagcgg aatacgccgc
     3541 gttcggcttc gacggtaacc gcctccgtct cccagagcat tgccgcgcgc tccttcatct
     3601 gccgaagcac atcgcgcgcc gcctcaatgg ctgcccagcc tgtggcgaac gtcgtacggc
     3661 tccctccggt cacggcagtg taaccgacgg agtccgtgtc caccaccgac ggcagcacgt
     3721 cctccgccgg aatgccgagt acttcggccg cctgcatggc caccgacgtc cgtgtgccac
     3781 cgatgtcggt ggagccttca acaaggctca cggttccatc cgcgttgacg ccaatcgtac
     3841 aactcgacgg aaagccgaca ttgaaccaaa agcccaccgc gacgccgcgc ccgcggttcg
     3901 ggccttcgag cggcgccgag tagtgcgggt gctgcttcat cgcttccagt acctcgacgc
     3961 agccgatgcg ctggtatttc ggcccgtcgg cccgcctggt gccttctttc gcggcgttcc
     4021 ttagccggaa ctcgatcggg tccatcgcga gtttttcggc taattcgtcc acgaccgact
     4081 ccgcggcgaa cgccgcattg gtcgctcccg gcgcgcgata cgccgccgtg ctgggcttgt
     4141 tcaccaccac gtcgaagccg tcgatcacca cgtttgggat gtcgtatgcg gcaaagatgc
     4201 acatcgcccc gggcagaacc atcgagccgg gatacgcgcc cgcctcgtac gccatgtatg
     4261 cttgcgcagc gatgatccgt ccatccttct gcgccccgat ctttacacgg acatacgaac
     4321 ccggcgtggg accgctgccc tcgaaaacat cccgccgcga catgacgatc ttgaccggac
     4381 gcccggcctt tttcgaaagc agcgccgcca ccggctcaag gtagacgttg atcttgccgc
     4441 cgaagccgcc tccgatctcc attggcgtca ctttaattcg tgaaaccggc agatccagca
     4501 cgtgcgaaag cacgtcgcgc acgttgaagg cgccctgcgt gctgcaccag acgctgacgc
     4561 gcccgtcgtt gttccacagt gccgcggcgt tatgcggttc gatgtaaccc tggtggacgg
     4621 tggaagtgtg gaactcccgc tcgacgatga catcggcggc agcgaacccc tgctcaccgt
     4681 cgccaagggt ccaccgaaag tgctcggcga cattgctcac tttgccggta tcctcgccga
     4741 attctttcgt cttaaggtcc ccgagcagaa ctggtgcgcc ttcccgcatc gcttcaggag
     4801 ccgtcagtac gcacggcaac gggtcgtagt cgatcttgat gagtccggcc gcctcttcgg
     4861 cggcgtgcgg tgtcgcagcc gcgaccgccg cgatcgcatg tcccctgtag agagccttgc
     4921 cgcttgcgag aaggttgccg cgaacgtagc tgaggggcac aggcccgtca ccaagatcca
     4981 ccatccgatc cggaccctgc ggaaaatcgg cggctgtgac cactgcccgg acgcctggga
     5041 acgattcggc cttgctggtg tcgattcgcc ggatgcgggc atgcgcgtgt ggacttctca
     5101 ccacgaatcc atgcagcatg cccgcggctt ggaagtccgc gccgtagagc gcgcgcccgg
     5161 tgaccttatc gtagccatcg tgccggaccg gccgggtgcc caccacggtg tattcgtgcg
     5221 gtttcatccc ttcacctccc gcaggtcggc ggccgcttgt tcgacggcgc gcaccacctt
     5281 gtcatacccc gtgcaccggc aaaggttgtt ggcaagccat tgccggatgg actcttcgtc
     5341 cggctcaggg ttacggtcca gcagcgcctt ggtggcgaca atcaggcccg gtgtacaaaa
     5401 gccgcactgg agggccgcgc tctcgaggaa cgccttctgc accgggtgta gttcaccgct
     5461 tcgggcgatt ccttcgatcg tggtgatgtt ccggccttcc acctcaaccc caagcacgag
     5521 gcacgagttg acgatgtcgc catcgagaat cacagtgcag gcgccgcaat tgccgtcgtt
     5581 acagccttcc ttcgtgccgg tgaggccgac gatgtcccgc aggcattcga gcaggctttg
     5641 acgcggctcg caaagaaatt cgactgcctc gccgttaacg gtagttcgca cgtgcacctt
     5701 gcggtgtgtc atatccggcc tcctgtggct cgctggacag cggcttcgag cgcgcgtcgt
     5761 gtcagcacgg cgcagagatg gcgccggtac tcctcggtac ctcgcatgtc cgtgatgggc
     5821 tgggcggccg cccgcgcgat ctcagcagcc gcgtggatgc tttccggcac ggcaggcttg
     5881 ccggcgagcg actcgccggc ctcgggaacg aagagcggag tgggagcaac cgcggcaaga
     5941 gcaatgcgcg cggtgcggaa gtggccatca tcaagcacaa cctggacgcc cgcgccggcc
     6001 accgcgatgt ccatttcatt gcgaggaatg aatcggagat acctcgcgcc ggagcccgcg
     6061 ggcggcgggg gcaactggaa tgacacgagt agttcgcccg gttcgagcac cgtgcggccc
     6121 gggcccgtgc agaaatcctc gacacgcacc tggcgggtgc cgttcgcccc tgcgatgcag
     6181 cagattgcac cgtaagcgat cagcatcggc actgcgtcag cgctcggcgc cgcgttgcag
     6241 agattgccgc cgagcgaggc acggctctga atctgcacgc cgccgataag tgccgcaact
     6301 tcggcaagag ccgggtaatg ccgccgcacg gaggcatttt cggagatgcg gcaacaaggg
     6361 gctgcagcgc caagggtcag cccgcgagcg ctgtcgtacg agagttcgtt gagttcagga
     6421 acccgcttga tgtcgacgac caggtcagtc tcaacccggc ccgcgcgcat ctgcactagc
     6481 aggtcggtgc cgccggcaag cgccttcgcc cgcgggcccg aggcgacagc gttcatggct
     6541 tccgccagcg tgaccggggc tgtgtagtcg aaccacttca tggcggggat ctcctttgtg
     6601 gcggttcttc agagcttcaa gctatctgag tatagccaac cgccgtcgtt cacagctttt
     6661 ccatctcttc gagcttcctg acgcggcggc ggaattcttc gctggtgctg aacttcgcgt
     6721 tgaggaaggc atgcaggatc tcttcggcca ccttgatgcc aatgatccag ttgcccacgc
     6781 agaggacgtt cacgtcgtcg tgctcgacgc actggtgggc cgagtacgtg tcgtgacaga
     6841 gagcggcgcg gatgcctcgg atcttgttac acgcgatggc cgcgccgatc ccggtgccgc
     6901 acaccatgat cccccgatcg gctcgcccac ccttgatctc cgcgcaaacc tgtcgtgcga
     6961 tatccggaaa atccaccggc tgggtactga agcaccctag gtccttcacc gagtgtcccc
     7021 aggatcgcaa catctcgatc accggcccct tcagtggaaa ccccgcgtgg tccgacccga
     7081 tcgccagccg catcgcacgc acgcccggag cggccgccac cgcacccgca accccgctcc
     7141 cgagaaacac ccgtctgcca atcccaccgc tcgatgaatc catgatccac ctcccgaagg
     7201 ggtcatttta cgctgctgcc agaggcggag gaatagcggc ttggaagctt tgcaaaaaac
     7261 tggggacact tcgggctggt ctcctgctat ggtccacttg cgacggtacg cagacgtgtc
     7321 cccagtttct ttacagcttc caggcgtcaa acgactcggc tcgaggggga tcagggatca
     7381 gtgagtggtg cggacgagag gacttgaacc tccacggggt ttcccccact agcacctcaa
     7441 gctagcgcgt ctgccaattc cgccacgtcc gcgtgtggca acggagggcg ttttcacgcc
     7501 cagccgctca actactgtac tacttctgtg gcgccggttt cgcgggggca ggcttcgccg
     7561 gagctgtctg cgttggccgc ggcaactgta tcgggatacc ctgcgatttc ccaccctgcg
     7621 gaatcgtccc cacgccgccc tgaggcgccg gaatggacgg cgttgacggc gtggtcgtct
     7681 tgcccggagt gtgcccttcg agcaccgtgg acccaacccc ggccgtccgg gtagccagga
     7741 tcgccaacga gatcgacgtc accatgaaga ggaccgccgc ggtggtcgtc gccttcgaga
     7801 gcaccgtcgc cgaaccgcgc gggccgaacg ccgtctgcga acccatgccg ccgaatgccc
     7861 cggccaaatc tgcggccttg ccgctttgca gcaacaccac aatgatcaga aacagacaga
     7921 cgataacgtg aatgatcgtg agaaggacta ccatgtgtct tgctttctaa cgagcggaga
     7981 tcggggctgg cgccggaacg acctgtactc tcctctctag tataggagac gggccgccgt
     8041 caggcaacgc acctgcaccc gccgttcgca ccctagcggt acgccggaat ccccgtaacc
     8101 tgttcgccga tcaccagcgc atggatgtgg tctgtgccct cgtaggtctt cactgtctcc
     8161 aggttgcaca ggtggcgcat gatcggatac tcgtccgtga tcccgttggc tcccagaagg
     8221 tcgcggccca gccgggcgca ctcgagggcg atcgccgcgt tgttgcgctt cagcatcgag
     8281 atgtgggccg gttcggcctt cccccggtcc ttcagccggc ccacctgaac cgccaacaac
     8341 tgcgccttgg tgatctcggt gatcatccag accagtttct cctgaacaag ctggtgcgag
     8401 gcgatcggcc ggtcgtcgaa ctgctttcga atcagcgtgt aacggcgtgc cgtctcataa
     8461 cagtccatcg ccgcgccgat cacaccccag ccgatcccat ggcgcgcttg ggtgaggcac
     8521 atcagggctg ttttcaagcc cttggctccg ggcagaacgt tctccgccgg gatgcggcag
     8581 tccgagaacg acaggctgga ggtcaccgat gcccgcatcg accatttccc gtgaatgtcg
     8641 gaggtcgtgt accccgccgt gcctctctcc accaggaagc cgcggacgcc ttcctctgtg
     8701 cgcgcccaga ctacggccag atcagccacc gagccgttgg tgatccagct cttttcccca
     8761 ttgagcaccc aggcgtcgcc atcgcgacgc gccgtagccc gcatcccgcc cggattcgag
     8821 ccgaaatccg gctccgtaag tccaaagcag ccaaggactt cgccggactg gaggcgaggc
     8881 agccatttcc gtttctgctc ctccgaaccg aacgcgtgga tggggtacat caccagcgcg
     8941 ccctgcaccg agacgcaact gcgcaccccg gagtccccgc gctcgagttc ttgcatgatc
     9001 agcccgtact cgacattgct cattcccggg cagccgtacc cttccaggtt cgctccgaag
     9061 aaaccgagct tggccatctc ggggatcagc tccataggga accggccctc gcggtagcaa
     9121 tcgcggatta ccggagtaat cctgtcatca acgaattggc gcgccgtttg gcgtaccaga
     9181 agctcgtccc cgttgaacag agagtcgata tgaaggtagt cgacgccgcg gaatggctcc
     9241 atgaccccat cttaccgtgc gaatgttctc ttccttccgc cactagtctc atagcccctt
     9301 gagggtaaga aggtgttacg accgatcctg ctattgcccc tgagcgtgct cgcactttgc
     9361 ggccagatcc cgaaggatcc ctccgcccgt ctgctcgacg aagcctataa ggcgcttcgc
     9421 gcaaaacact atgactccgc catcgagtcc ttccggaagg cgatcgacct tgcgcctgac
     9481 cgtgccgccg tccgcaagga cctggcatat acgtatttga agatcggcga aaccgaagcc
     9541 gctcgtggcc agttctccga agcgatgcgg ctcgcgccgg gggatcacca cgctgcgctc
     9601 gaatttgcct ttctttgtta tgaaaccggc aagaccgtcg aggctcggcg cgttttcgac
     9661 cgcgtccgca tgcaaggcga tcccgcctca cgcgaaacgg cagggagggc tttccagaat
     9721 gtcgaccgtt cgctcgccga gggaatcgag cagtggaccc gggcgctcgc gctccatccc
     9781 gatgacttca gcgctcatca cgaactggcc agactcgccg cgcaacgaga tgactggaaa
     9841 ctcgccgcgg cacacttcga aagggcttgg caactcaggc ccgatctgcg gtcggtgctc
     9901 gtcgaattgg ggcgggcccg aagggagttg ggcgaggagg agcgcgcgat ggccgccctg
     9961 ctggccgcat cgcgcggagc cgagccgcgt gcggcggagg acgcccgctc tctcatgccg
    10021 gcacgctacc cgtatgtcga cgagttccgg caggcgctca gcctggatcc caagaacgtg
    10081 gaacttcgcc gcgaacttgc ctacctgctg ctcgaaatgg gcaaggcgga ggaggcgaag
    10141 gccgagttcg cggcgctccc ggcggccgct ccggctgagc ctcctggcaa ggccagcatc
    10201 gcgccgaagg aaatggcgcg gcgcagctat gagaaaggct acctgcaaga tgcgctcaca
    10261 tactactcgg ccgcgcaaga agctgacccg ctcgacttcg gggtgatgct ccagttggga
    10321 tggaccaata acctgctgcg cagggaccgc gacgcgatcc ggtggttcgc tctggcgcgg
    10381 agaagccccg aaccggcaat cgcggccgaa gccgccaagg ccgagcgtaa cctgaagcca
    10441 cgtctgacgc gctttcgcac gagcgcctgg atcttcccga gctactcctc ccgatggcgg
    10501 gacgtgttca cttacgggca actgaaggcc gacttcaagc tcagcgcact gccactccgg
    10561 gcatacgcct ccattcgctt tatcggtgat acgcggacgg cgatgggcga cgccccgccg
    10621 caatacctct cggaaagctc ggtcattctc ggagtggggc tcgccaccga ttactggcac
    10681 ggattgatgc tctggggcga ggcgggtagc gcgatcaact accttggcaa ccgccgcggc
    10741 gcgccgcgaa tggcaccgga ttaccgcggc ggcctggcct tctatcgcgg cttcggacgg
    10801 ctgattggca cccccgcgcg cggcatgttc ttcgagacga atggcgacgg cgtcttcgtc
    10861 agccgcttcc aaaacgacgt cgtgctgtac tcgcagaacc gcttcgggct cacactaccc
    10921 gccgcgaatt ccctgggcgg gctcgaagcc caactctact ggaacgggaa cgccagcacc
    10981 gacctgcgtc gacagtactg ggccaatgtc gtcgaaactg gacccggtct ccggttccgt
    11041 tggaagggcc tgtcgccctc gtggctgttc tcggtgaact tcctccgtgg cgcccacaca
    11101 cgcaacgtcg gcaatccgcg gagcccgaac ttcttcgacc tgcgagtcgg catctggtat
    11161 tcggtcacgc ggtgaaatga cgatgatcct cagacgggca gcgccgcacg cactctgcgt
    11221 ccttcttcag gcaatctcat gcctggccgc ttcattccat gtggttggta atgaccttgg
    11281 cgcctggcca cgtatcctag gatcggtcgg ttttcagaca acatccggcg gagagggtgg
    11341 gattctgctc gctcgggaag gaaaggccga agcagcctcc gattggatgg aacgggtcaa
    11401 gcgtggtgcg tacctgatcc tggagggcga ctcccctctt gcgggggagc tcggattccg
    11461 ccctggccct ggacgggtcc acgtttcgag tgtcgaggac ctgcgccgtc ccggattgtc
    11521 gatcgtctgg gagtcagcag tggatatccc gatcttcgaa atgccccagg atgctcgtgt
    11581 gttcgctcgg gaacgctggc ggaaggcgcc cctgctggcc gggttccgcc gcgggcaagg
    11641 cgccgttctt tggctcgctg tctcgcccgg acagcgcggc tatgaacggt tcccctatct
    11701 cgttcaggcg cttgcggacc tcggcctgga gccaccgttc cgctctcgcg gcctgtgggc
    11761 gttcttcgat tcctcgtacc gtgcgcgtgc cgacctcgag tacctcgcac gccggtggcg
    11821 agcggccggc atctccgcgc tccaggtcgc cgcctggcac tatttcgagc ccgacgaatc
    11881 ccgcgaccac tacctccgga cactggtcga gagttgtcac cggcagggca tcctcgtcta
    11941 cgcgtggctc gaattgcccc acgtgagtga ccggttctgg aacgatcacc cggagtggcg
    12001 cgagcagacc gccctgcttc aggacgctcg tctcgactgg cggaggctga tgaacctggc
    12061 gaaccgggac tgcttccgcg ccgccgcaac gggagtgcgc cacctgctgg ggcgtttcga
    12121 ttgggacggc gtcaatcttg ctgaactgta cttcgagtcc cttcagggcc acgcgacgcc
    12181 ctcccgtttc acgccgatga acgccgatgt gcggcgtgag ttccgtgacc gggccggttt
    12241 tgacccgctc gagctgttca atcccgcgtc tgcccgccat tactcccgta acgagtccga
    12301 tctccgggac ttcctgggct accgggtcga tctggtgcgc cgcattcaaa ccgaatggat
    12361 cggcgaagtc gagtctatgc gccagtccaa gccggatctc ggtcttgtgc tgactcatgt
    12421 cgacgacgga ctcgatccag gcattcgaga cgcgattggc gccgatactg cggcgttgct
    12481 gccgcttctc gcccgtcaca gtcttacgtt tctcatcgag gatccggcca cggtctggaa
    12541 tttcggcccg gaccgctacc ggcagatcgc tctcaggtac gcgaggatta cgccgcatac
    12601 cagcaggctg gggatggaca taaacatcgt cgaacgctac caggacgtct atccgacgaa
    12661 acagcagacc gggggcgaac tctttcaact cgtgaacacc gcctcgcgtg tgtttcctcg
    12721 agtggcgctc tattttgaga actccatcct ggcgccagac atcccgtggc tcgcctcggc
    12781 ggcagccacc gttgatcgat tcgagtgcgc aggcacgagg gtagccgtcc agtcgccgcg
    12841 cggcgccggc gttccatgga agggaccggc gttggtggat ggtcgcccgt ggccggccta
    12901 cgacggcgaa actgtatggt tgcccgcggg ctcacatgca atcgagccgg cggaagggga
    12961 gccccatgcc cgcctgctcg atttcaacgg cgatctcgtg gctgccaggt acctgactga
    13021 tgggctggag ttctcttacc gcagcgatgc ccccgcattc gccttccttg accggcgccc
    13081 cagggcggtc gaggtcgatg gcgaagtttg gaaaatccgg ggagtcgact cggagggacg
    13141 gcacatgatc cgtctcccgc gcgggcagca cctggttacg gtgaaaacaa ggtagcaaag
    13201 ctaccgctgt gcaacgacgg tagccggggg acccagtgct acgacctcgc ctgccttcat
    13261 gccggacaaa accgcgacgt gagtgaagct ccggaggccc aactggacct ctcgtttatc
    13321 gaagcggtcc ccgtctttaa caaagatggc actcttacca ccctcggtat gcactgcctc
    13381 gagtggtgcg atcagcgtat tctcctggtg gccaaccgta accgtcgcgg ccgcggacag
    13441 gtccgggatt agccggggat cggcaccctc aatcgtgacg cggaccggta cccggcgaat
    13501 atagtagttt tcccgccacc cgcgcacact catcgcaccg atgctgaaca ctcggcccgg
    13561 aaacgcgacg ccaggaaatg catcgagtgt gacggaggct cgctggccga cccggagatc
    13621 attgctttcc gtctggttgg cgtccgcctc aacctgcatg ctggagacat ccacgatctt
    13681 catgaacgtc tgcccggggt aaacctgatc accgagctga atctgcgaca tctctccggc
    13741 ccgaaagaac gttgacatga ccgccagccc gtcaattggc gcgatgaacg tgagcctctt
    13801 caagtcgata aggtggcgct gccgatgcat ttcatggcgg cgccgggtta tttccagtat
    13861 tcgcaattcg gcacggtgta cggcgcgcgt cttttccaag tcctgctgca actgcttgta
    13921 ccgcgcttcc gactcctcga cgaagagccg cagtagttct tgatcaacta ccgtgcgcac
    13981 tgcggaggcc gaattgtcca acctcgcctg gtcgagctca gccttggcaa cccgaacgtt
    14041 ctgctggagg ttctcccaat cgatcgcttg ctctgcccgg cgctttctga tgtccgtctc
    14101 ggcctggatg acaatcgaat taacgtcgtc gatgtggtcc cgagcggctt gcgtatcgag
    14161 ctgggccacc aactggcctt tcttaacgaa gctcccggac ttcgccagtt cgaccaggat
    14221 aagttcccgc cccgagtccg gcccgcgcat gagcggaacc gtgatggagg cgaagttccg
    14281 ggcggcggtc tgcccggaga ggcggactac ccgttcgacc gcgcccctgg caaccgttgc
    14341 agtccggatt gcggcggccg ttgacgccgt ttgttgcggt ctggagagct gccagtacat
    14401 cgccacggca atactcacga gcacagccag aatgaaccaa aggctccacg gtttgggggc
    14461 cttcggcggc gcaggagagg gcttcgggcc ttcctggacg ggcgttccgg ctggcgaggg
    14521 agagggattg ccgatagcag acatctctag tttttagcgt acaccactac tgctcactga
    14581 agaggcgaag ggcgtcgtac cgaggttacg ccccagatcc gtggctgggc gcgcggcgcc
    14641 tcgaagaacg ctgatgtctt cgtcctgagc acattgcgga gagtgacgaa ggatctgcag
    14701 gctgagccgt tattggggat ttcggaggtt gtggtactgg gattaacctt agtaatcaaa
    14761 gcgttgttac ctattgggat tattgacctt agcccaatgc gtgatagatt taactttgca
    14821 gttcactgga ggagaactat cctcaattgc ggggtgagca cgtgctaaga ggaaaattcc
    14881 tgtttttcgg tctgatggtg tgccttgttt cggcagcctg gggaggaacg tttggcaagg
    14941 tcgtgccgat cggtggtcac gcctccgata tcgcgctcga cgaacggcgt ggggtggtct
    15001 acctcgccaa ctttacggcc aatcggattg aggtgatttc catctccgac tattcacgca
    15061 aaacgcccat ccgggtcccg aggcagccgg gttctatcgc cctgtcgcca gatgcccgct
    15121 acatggtcgt cacgcatttc ggcaagtggg acgcgacgtt cacgatcaag ccgtcggtca
    15181 cgatcctcga tctcgatacc aatcaatcgc ggaacatcga tctcggcgac aacccgctca
    15241 cggtcgcgtt tggaggcggc tcgcgggcgt tgctcgtcac ggaaggcacg gtcgctgccg
    15301 ccggacgatt cctgctgtta gatcccgcat caggatcgct ggaggtgctg gcgccggccc
    15361 ttgtgggcgc cgaccagcta ccggtcccgt ttgccacctt cccgccggag atcatcaagg
    15421 cgtcgggggg agtgtcgggc gacaaccggt ggatcatcgg catggcctcc gcacaacagg
    15481 ctgagcaggc acttaccgtt caatatcggt acaacgtgca gaccaggcag ttgagcgtga
    15541 tctttatcac atccgcgcca ccgctcggcc cgcgcgtggt cagcgtcaat caggacgcct
    15601 cgatgagcct tctgg
//
LOCUS       DATIFQ010000398         3398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_130600, whole genome shotgun sequence.
ACCESSION   DATIFQ010000398 DATIFQ010000000
VERSION     DATIFQ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130600"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VLE22_19395"
     CDS             <1..1161
                     /locus_tag="VLE22_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16625.1"
                     /translation="DEVWQRIAFLPAELKPATPKESGWPLARHFRGAGHVHMLGAWDD
                     PNATWAFFGAGPTYAGHSRDDEGHFFISKCGHLANRSGGAGHNDGWYYSGGSLIYNIV
                     TVYHPEEKMRRNAYNENDGGLIRHVYENGFPKERGNITAYWHDDRLATYASADLTKGY
                     WSEKVREVTRQFLYLRGAQECFVVFDRVESTDAKLPKTWFLHLPSEPSVSGTAGVKVA
                     DHVFEYDGDTATWLSDPAGDTGLLASGRSRMTLRTLTPEAARITKRGGEGHDFWGHPH
                     NPAAQYNHTLDKDGKEDRAYRRPPFSPWRLEVEPAGVHERDYFLHLLYVTDEATVAPA
                     QAERIEEEGRLGARIRLGSRAVTVIFQTTGPLGGHLKIEEAGRTLHNAALPG"
     gene            complement(1172..2356)
                     /locus_tag="VLE22_19400"
     CDS             complement(1172..2356)
                     /locus_tag="VLE22_19400"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HSB16626.1"
                     /translation="MATKRIGILTGGGDVPGLNAVIKGVVYRGSENGCEVVGIRRGWE
                     GLTHVNLEDPVSQARYILPLNRENTRTIDRTGGTFLHTTRTNPAKMKSLPDHLKGMEF
                     PKAESTKKGVTSIVLDVTNQVLKNLEILRLDYLIAIGGDDTLSYAARLDKEGFGVIAI
                     PKTMDNDVQNTEYCIGFSTAITRAVDAIERQRTTVGSHERVGVFRVFGRDAGYTALYT
                     AYVNSIRCAIPEYKFNLERMIELLIADKRSNPSNYSLVVLSEGAEWEGYQVKEYGEPD
                     AYGHRKKMSVGEDLSGEIKKRTGEETVVSDLTYDLRGGSPDFVDKLVATTFGNMALDA
                     IQDNKSGVMTAIVNGCYSLVEIPDPKRGPRKVDIPTMYNTERYRPKYDLKLGLPIFLT
                     KV"
     gene            complement(2421..>3398)
                     /locus_tag="VLE22_19405"
     CDS             complement(2421..>3398)
                     /locus_tag="VLE22_19405"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSB16627.1"
                     /translation="EYSYTVAVNGREIGAGAVRTLPEHSDRLAFLVIGDYGTGGRAQY
                     EVASAMAKVVRERSTTDNPVRFVLTTGDNIYAYRWLGFIRTQSGDRDRHWRAKFFEPY
                     SDLLLHVPFYPTLGNHDGGESESDGDLGVYLDNFFFPTPKPSRYYSFSVAGLADFFAL
                     DSTDIATSERQPIYAPGGTESRWLEKSLAQSKAPWKIAYFHHPPFNAGPGHGPSLDQL
                     RHWVDLFGKHGVQIAFSGHEHNFQWSKRNERTAGVQFVITGAGGELRSRDVRRRLEAA
                     NIAAWAPQCHFLLVEIEGGSMTVNVLGSSDVMVRDHEGRRVSLPLRVTL"
BASE COUNT          644 a         1041 c         1042 g          671 t
ORIGIN      
        1 gacgaggtgt ggcagaggat cgcgttcctg cccgccgagt tgaagccggc gacaccgaaa
       61 gagtccggct ggccgctggc gcgccacttc cgcggcgcgg gacacgttca catgcttgga
      121 gcgtgggatg acccgaacgc cacctgggcg ttcttcggcg ccggtccgac ttacgctggt
      181 cactctcgtg acgacgaggg ccactttttc atctcgaagt gcgggcacct ggcgaatcgc
      241 tccgggggcg ccggccacaa cgacggctgg tactactcag gcgggtcact tatctacaac
      301 attgttaccg tgtaccaccc cgaggagaag atgcgccgga acgcgtataa cgagaacgac
      361 ggcggactca tccggcatgt ttatgagaac ggttttccga aggagcgcgg caacatcact
      421 gcatactggc acgacgaccg gctggcgacc tacgcgtcgg ccgatctgac caagggttac
      481 tggtctgaga aagtgcgcga ggtcacacga caatttctat atctgcgtgg tgcgcaagag
      541 tgttttgtgg tctttgaccg cgtcgagtcc accgatgcga agctgcctaa gacgtggttt
      601 ctgcatctcc cgtccgagcc gtcggtaagc ggcacggctg gggtaaaggt agccgatcat
      661 gtcttcgagt acgacggtga cactgcgacc tggctcagtg atcccgcggg tgacaccggg
      721 ttgctggcgt ccggccggag ccgcatgacc ctgcggacgc tcacgcccga agccgctcgc
      781 atcacgaaac ggggcggaga gggacacgac ttctggggcc acccgcacaa cccagccgcc
      841 cagtacaacc acacgctgga caaggatgga aaggaagacc gcgcgtatcg ccgcccgccg
      901 ttctcgccgt ggcggcttga agtcgagccg gccggcgtac acgagcgcga ctattttctg
      961 cacctgcttt acgtaaccga cgaggctacc gtcgcaccgg cgcaggccga aaggatcgag
     1021 gaggagggcc gccttggcgc gcgaatccgc ctgggttcgc gtgcggtcac tgtgatattt
     1081 cagacgacag gcccgctcgg gggtcacttg aagattgagg aagccgggcg aacgctgcac
     1141 aacgccgcgc tgcccggctg aaacagacgg cttacacctt agtcagaaag atcggcaggc
     1201 cgagcttgag atcgtacttc ggacggtagc gctcggtgtt gtacatcgtc ggaatgtcga
     1261 ccttgcgggg gccgcgcttc ggatccggga tttcgaccag ggagtagcag ccgttgacga
     1321 tggccgtcat cacaccgctc ttgttgtcct gaattgcatc gagagccatg ttgccgaacg
     1381 tcgtcgccac cagtttgtcg acgaaatcgg gcgaaccgcc gcgaagatcg taggtgaggt
     1441 cgctgacgac cgtctcctcg cccgtgcgct tcttaatctc gccgctcagg tcttcgccga
     1501 cgctcatctt tttgcgatgt ccgtaggcgt caggctcgcc gtactccttt acctggtatc
     1561 cctcccactc ggcgccctcg gagagcacaa ccagcgaata gttactggga ttgctgcgct
     1621 tgtcagcgat cagaagctcg atcattcttt cgagattgaa cttatactcg gggatcgcac
     1681 aacggatcga gttcacgtag gcggtataaa gggccgtgta accggcgtcc cgtccgaaca
     1741 cacggaacac gccgacacgc tcgtgcgagc ccaccgtagt ccgctggcgc tcgatcgcgt
     1801 cgacggctcg ggtgatggcc gtcgaaaagc cgatacagta ctcggtgttt tggacgtcgt
     1861 tgtccatcgt cttcgggatg gcaatgacgc cgaacccctc tttatccagc cgcgccgcgt
     1921 aactcagggt atcgtcgccg ccgatggcga tcaggtagtc gagccggaga atctcgaggt
     1981 tcttcagcac ctgattggtc acgtccagga cgatcgacgt tacgcccttc ttggtgctct
     2041 ccgccttcgg aaactccatg cccttgaggt ggtcgggcag gctcttcatc ttggccggat
     2101 tggttcgagt ggtgtgcagg aaggtgccgc cggtacggtc gatggtgcgg gtattttcgc
     2161 ggttcagcgg caggatgtac ctcgcctggc tgacggggtc ttcgaggttg acgtgcgtca
     2221 gcccctccca gccgcggcgg ataccgacca cctcgcagcc gttctcgctc ccgcgataca
     2281 ccactccttt gatgacagca ttcaacccgg ggacatcgcc gccgccggtc aagattccta
     2341 ttctcttcgt cgccatggac actcctgtag tttgatcttg aggacttaac aaacgaaaat
     2401 gacggggaac gtcttacgta ttatagcgtt acccggagcg ggagggatac tcgcctgccc
     2461 tcgtgatcgc gcaccatgac gtcgctcgaa ccgagcacat ttacggtcat agacccgcct
     2521 tcgatctcga ccagcagaaa gtggcattgc ggggcccaag ccgcaatgtt cgccgcctcg
     2581 agccggcggc ggacatcgcg ggaccgcaac tccccacccg ccccggtgat gacgaactgc
     2641 acgccggcgg tccgttcgtt acgtttgctc cattggaagt tatgctcgtg cccgctgaaa
     2701 gcgatctgaa cgccgtgctt tccgaacaag tcgacccagt ggcgcaattg atctaagctc
     2761 ggtccatgtc caggccccgc attgaacggc ggatgatgga agtatgcgat cttccatggc
     2821 gccttggact gagcgagcga cttttccagc caccgggact cagttccgcc tggggcgtag
     2881 atgggctgcc gttcactggt agcgatatcg gtcgagtcca gcgcgaaaaa gtccgccagg
     2941 ccggcgacgc tgaagctgta atatcgcgac ggtttcggcg tcgggaaaaa aaagttgtcc
     3001 agatagacac ccaaatcgcc gtccgattcg ctctcgccgc cgtcgtgatt tcccagcgtc
     3061 ggatagaacg gcacatggag gagcaaatcg gagtaaggct cgaagaactt cgctcgccag
     3121 tgccggtcac ggtctcccga ctgcgtccgg atgaaaccga gccaccggta tgcgtagatg
     3181 ttgtcgccgg tcgtgaggac gaaacggacc gggttgtctg tcgttgaacg ttcccggacc
     3241 acctttgcca tcgcggaggc cacttcgtac tgcgcccggc ccccggtccc gtagtcgccg
     3301 atcaccagaa aggcaagccg gtccgagtgc tccggcagtg tgcggacggc cccggcgccg
     3361 atctcacggc cgttcacggc gacggtgtag gaatactc
//
LOCUS       DATIFQ010000399         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1090664, whole genome shotgun sequence.
ACCESSION   DATIFQ010000399 DATIFQ010000000
VERSION     DATIFQ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090664"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1936)
                     /locus_tag="VLE22_19410"
     CDS             complement(83..1936)
                     /locus_tag="VLE22_19410"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HSB16628.1"
                     /translation="MTDRSRLSKLTSLLERIDRRIAHGVHASATLTRWRLVLFLAGLV
                     CTVTLYKLGWYNSGNLALAGFVALFIAVAAYHNRLENRIHRLRQWKTIKAVHRARIRL
                     EWAAIPLRSSEQPPSHLYANDLDLIGLHSLTHLLDTTVSDHGRERLRSWLLSQPPDSA
                     VWAARQELVRELTPRALFRDRLVLEAQLAGEQEINGRRLAAVLEHPIGIPRLNLILAA
                     QSLLALATISLGIGALLSWLPGYWMFSFGVYALIYLMTDQGEELLEHAVGLHHEMERL
                     GTVLNHVEYYARRRDSRLAAACTSLMLGRARPSRHLNRAAKVLHAISIKAHPLVHLAI
                     NALCPWDLWFTRRLQQVQRDIQHHLPSWLDCLAEIEAASALATFAYLHPAYVWPASLT
                     ATGEQDGAGAALRADRLGHPLLPDDTRVTNEVQLAGLGSIHLITGSNMSGKSTFLRTI
                     GINLCLAQAGAPVCARRFEWTWSRLACCIRINDSLDAGLSFFYAEVKRLKMILDATGE
                     RAAPPVLFLIDEIFKGTNNRERLIGSRAFITALAKGNGLGLVTTHDLELTDLDKMIPH
                     LTNAHFQETVASGALQFDYQLRPGPCPTTNALRIMELEGLPVPHPPNSPNS"
BASE COUNT          420 a          573 c          753 g          412 t
ORIGIN      
        1 cttgagtcat gggatgtgtg cggaaatctg tcgcaaacgc gcgtataaat atggaggaag
       61 tgagaacaaa ggtcaaagaa aactatgagt taggggagtt cggaggatgg gggaccggga
      121 ggccttccaa ttccatgatg cgcagcgcgt tggtggtggg acaggggccc gggcgcagtt
      181 gatagtcaaa ttgcagcgcg ccggaggcga cggtttcctg aaagtgcgcg ttggtcagat
      241 gcggaatcat cttgtcgagg tccgtgagtt cgaggtcatg tgtcgtcacc aggcccaggc
      301 cgtttccctt cgcgagtgcc gtgataaagg cgcggcttcc gatgaggcgc tcgcggttgt
      361 tcgtgccttt aaaaatttcg tcgatgagga agaggaccgg cggcgcggcg cgctccccgg
      421 ttgcgtcgag aatcatcttg agccgtttca cctcggcgta gaaaaacgac agcccggcgt
      481 cgagcgaatc gttgatccga atgcagcagg ccagtcggct ccaggtccac tcgaatcgac
      541 gggcgcagac gggcgcgccg gcttgtgcga ggcacaggtt gatgccgatt gtccggagga
      601 acgtactctt gccggacatg ttcgagccgg tgatcaaatg gattgaaccc agtccggcca
      661 gttgtacctc gttggttacc cgcgtgtcat cgggaaggag cggatgaccg agccgatcgg
      721 ctcgaagcgc cgcacctgcg ccgtcctgtt cgccggtcgc tgtgagggaa gccggccaga
      781 cgtaggcggg gtggagatag gcgaacgtgg ccagggccga cgcggcttcg atttccgcca
      841 ggcagtccag ccacgaaggg agatggtgct ggatgtcccg ctgaacctgc tgcaggcgtc
      901 gggtgaacca cagatcccac gggcagagag cattgattgc cagatggacc agcgggtgtg
      961 ctttgatact gatggcgtgg agcacctttg cggcccggtt taggtggcgg gaaggacggg
     1021 ctctgcccag catgagcgat gtgcaggccg cggccaggcg cgagtcccgt cggcgcgcgt
     1081 agtattcgac gtggttcaga accgtgccca gtcgttccat ttcatggtgg aggccgacgg
     1141 catgttcgag caattcttct ccctgatcgg tcattaagta gatcaaggca tagacgccga
     1201 acgaaaacat ccaataaccg ggaagccagc tcaggagcgc gccgattccg agcgagatgg
     1261 ttgcaagcgc caggaggctc tgcgcggcaa ggatcagatt gagccgcgga atcccgatgg
     1321 gatgttccag tacggcggcc agacggcggc cgttgatttc ctgttcgccc gccaattgcg
     1381 cttcgagcac gaggcggtct cgaaacaaag cccgcggggt aagttctctg acgagttcct
     1441 ggcgcgcggc ccagacggcg gaatcgggcg gttgggacag gagccagcta cgcaggcgtt
     1501 cgcggccgtg gtccgagacc gtcgtgtcga gcaggtgtgt cagcgaatga aggccgatga
     1561 gatccagatc gttggcgtag agatgcgatg gaggctgctc gctcgagcgc agagggatcg
     1621 cggcccactc caatcggatg cgcgcccgat ggacggcttt gatggttttc cactgccgga
     1681 gccggtgaat ccggttctcg agcctgttat gatacgcggc cacggcgatg aagagggcga
     1741 cgaagccggc cagcgccagg tttccgctgt tgtaccatcc gagcttgtag agtgtgaccg
     1801 tgcagaccag gccggccaga aacagcacca aacgccagcg ggtcaatgtc gcgcttgcgt
     1861 gcacgccgtg tgcgatccgg cggtcgatgc gttcgagcaa gctggtgagt ttcgacaatc
     1921 gggaccgatc cgtcatgggt gcgaccttac tggatgggcg ctcctccgtc aacgctgagc
     1981 atggaagatg acgtcgcatt gggtcgaact caggatcgat acgtcggttg acgcaggcga
     2041 actgcttggc ctgctcgctg atcctatggc gcagggtgcg tggcaggaga acggatcgat
     2101 ccggctctat tggctggacg gtcactggaa cagtgattgt ctggccagag tgcggtcg
//
LOCUS       DATIFQ010000400         3677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_898927, whole genome shotgun sequence.
ACCESSION   DATIFQ010000400 DATIFQ010000000
VERSION     DATIFQ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898927"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VLE22_19415"
     CDS             <1..570
                     /locus_tag="VLE22_19415"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSB16629.1"
                     /translation="GRRTSSYNFSYGANGANLRMTQFQAALLVAQMTRIEEQSKTREQ
                     NAAYLTSMLREIPGITPARMYGGCTRNAYHLYMFRYNKEQFAGAPRSAFLKALDAEGI
                     PGSSGYSPLNKEPFLKNAMASRAYRAIYPKELLAKWESRNQCPANDRLCEEAVWFTQN
                     MLLTSRRDMEQIAEAIKKIQALAGDLKRA"
     gene            complement(574..1284)
                     /locus_tag="VLE22_19420"
     CDS             complement(574..1284)
                     /locus_tag="VLE22_19420"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HSB16630.1"
                     /translation="MRTAAAAALTATACALIGCMPQTHRHAMREENRSIELGKVEMVR
                     ALVEMKAGELRIRGGAGDLLEASLRYDERAKPEIRYDTSGFRGDLTVRQPHVSFNIGS
                     SDNPNDWDLRLNDDVPMDLEVRVGAGKSVLELGSLYLRSLEVHLGAGEINLDLTGKWR
                     KSMEAHIRGGVGKAVVRLPKDVGIEARARGGIGSIRVSGLERSGDLYTNEPAVKSPVT
                     LRLDIEGGIGEIRLEAEE"
     gene            complement(1539..2111)
                     /locus_tag="VLE22_19425"
     CDS             complement(1539..2111)
                     /locus_tag="VLE22_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16631.1"
                     /translation="MSDLRAAWLCRRWVFAACLTVLFLIGATAQPPRANMNEAPDNAP
                     LKLVITSSRQRVMQNESFVLEAKLVNVSGERVSIFGQLLWGYAGGLTLHVSDQCGRPV
                     QAEQHDDDMVVPSVLGNPEFYVVLFPDHFLGIDRKDSPKNLFRKPGSYSLFAEYQSPV
                     PDRYAKAPNFWGRERGSIRSAPIRIEVIEK"
     gene            complement(2629..2994)
                     /locus_tag="VLE22_19430"
     CDS             complement(2629..2994)
                     /locus_tag="VLE22_19430"
                     /inference="COORDINATES: protein motif:HMM:NF026924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HSB16632.1"
                     /translation="MVAAEVKALYSLEGDNLEQYAPDAPDRFLLRIRAIVGPKGAPGE
                     ESFDIRVCTPAWLADVCEAEGFVVGRHYIVVANYDFAALESVITKLIESCSGNSWQEV
                     AQKVARIGYWEFEDYQPSG"
     gene            complement(3164..>3677)
                     /locus_tag="VLE22_19435"
     CDS             complement(3164..>3677)
                     /locus_tag="VLE22_19435"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSB16633.1"
                     /translation="ALHRGGFLHDIGKVAVPDSILYKNGRLTEDEWSVMKLHPVKGEE
                     ICRGMKSLKPVLPIIRSHHERWDGSGYPDGLKGEQIPLAARILQVADVYDALTTVRPY
                     KAALSSEKAFVILDQEVDRGWRDPDLVPLFRRLHRESLKNGDPAFLYSSQLELMNQSV
                     SGFSLAHFAD"
BASE COUNT          725 a         1118 c         1118 g          716 t
ORIGIN      
        1 gggcggagga catcgagcta caacttcagt tacggcgcca acggagcgaa cctgcggatg
       61 acgcagttcc aggcggcgct gctggtggcc cagatgacga ggatcgagga gcaatcgaag
      121 acgcgggagc agaacgcggc ctatctgacc tcgatgctgc gcgagatccc cggcatcacg
      181 ccggcgcgga tgtacggcgg ctgtacgcga aacgcgtatc acctctacat gttccgctac
      241 aacaaggagc agttcgcagg cgcgccccgc agcgctttcc tcaaagcact ggacgccgag
      301 ggcatcccag gctccagcgg ctactcgccg ctcaacaaag aaccgttcct gaagaacgcc
      361 atggcgtcgc gggcctaccg ggcgatctat ccgaaggagt tgctggcgaa gtgggagtcc
      421 cgaaaccagt gccccgccaa cgatcggttg tgcgaggaag cagtctggtt cacgcagaac
      481 atgctgctga cctcccggag ggacatggag cagatcgccg aggcgatcaa gaagatccag
      541 gcgctggcgg gcgacttgaa gcgcgcctga cccttactcc tccgcttcaa gacggatctc
      601 gccgatgccg ccctcgatgt cgaggcgcaa ggtcactggc gacttgacgg ccggttcgtt
      661 ggtgtagaga tcgcctgacc gctcgaggcc cgagacgcga atggacccga tgccgccgcg
      721 ggcgcgggcc tcgattccca catcttttgg caatcggacg acggccttgc cgacgcctcc
      781 acgaatgtgg gcctccatgc tcttccgcca ctttccggtc agatccagat tgatctcgcc
      841 ggcgccgagg tggacctcca gggagcgaag atagaggctt ccgagctcca ggacgctctt
      901 gccggcgccc acgcggactt cgaggtccat cggcacatca tcgttgagtc gaagatccca
      961 gtcgtttggg ttgtccgagg agccgatatt gaagctgacg tggggctggc ggaccgtcag
     1021 gtcaccgcgg aaaccgctcg tgtcgtaccg gatttcgggt ttcgcgcgct cgtcgtaacg
     1081 cagtgacgct tcgaggagat caccggcgcc gccccggatc ctgagctctc ctgctttcat
     1141 ctcgacgaga gcgcgcacca tctccacctt gccgagttcg atggaccggt tctcctcgcg
     1201 catcgcgtgg cggtgagttt gaggcatgca tccgatgagg gcacatgccg ttgccgtgag
     1261 ggcagccgcg gcggctgtac gcattgctag cctcctggat gagttaacga accatctggc
     1321 ggaaaagttc cctgtcagtc caggacatgc tacgcaatcg ccaaacttca ggccccgcac
     1381 aagttatcgc ttacttcgcc cgcgtgctgc ggtcgacgtt ccgcatcata tcactcaacg
     1441 tggcagcgct cgtgctcata cgccacgtac cggtggcggc cctgccggcc tgggtgagcg
     1501 agaacggtcc gtctgccgcg gcttccctcg ccggaacgct atttttcgat cacttcaatg
     1561 cggatgggcg cggatcgaat cgagcctctt tcacggcccc agaagttcgg agccttcgca
     1621 tagcgatccg gaacgggact ctgatattcg gcgaacaacg aatacgaacc cggtttgcga
     1681 aacagattct tcggcgaatc cttccggtcg atgcccagga aatggtcggg aaatagcacc
     1741 acgtaaaatt cggggttgcc cagcacagaa ggaacgacca tgtcatcgtc gtgttgctct
     1801 gcctgaaccg gccgcccgca ctggtccgat acatggagcg tcaatccacc agcgtagccc
     1861 cagagcaact gcccgaatat cgataccctc tcgccgctta cgtttacgag ctttgcttcg
     1921 agaacgaaag actcgttctg catgactcgc tgtctggaac tggtgatcac cagcttgaga
     1981 ggagcattat caggcgcttc gttcatattg gctctcggcg gctgagctgt cgccccgatg
     2041 agaaaaagga ccgtcaaaca cgccgcaaac acccaacgcc tgcacagcca cgctgctcgt
     2101 aggtcgctca tatggagttt ctgggacgtg agtttcagga acatactacg caatcgccag
     2161 ccttcatgct ccgcatcgtt atggctcgcg tcgcccgtgg ggttgcggtc gacgtctcgc
     2221 atcatatcac tcaacgtagc aacgctcgct gcgacgcgag tcagagagaa ccgcccggcc
     2281 ccggtcgtgc cggggagcgt tgactgaaca aagccaatcc cagtatcgga caccggcgcg
     2341 gcgcgtgcga ctggcgactg gcctcgatcc ggcgaaggga agacatcggg aaagcctcgg
     2401 atctggaagt tggggaggaa agcgggaacc ccgagttccc tcctgtccgc atgttacggg
     2461 gcgggatcgt aatcctgggg cgatggggtg gtgtcacccg gatctaagtc attccgagag
     2521 aacgagttga tgagttgggg gtggattgtg actgggagct gtggccgacc agaaatctgc
     2581 tcagttcagg ccgacgggtt tctccgcgcg tttctcatcc aacgaacctc agccggatgg
     2641 ctggtagtct tcgaactccc agtagccgat ccgtgctacc ttctgagcga cttcctgcca
     2701 cgagttcccg gagcagcttt cgatgagttt cgtgatgaca ctctccagag ccgcaaagtc
     2761 ataattagcg acaacgatat agtgcctgcc gaccacaaaa ccctcggcct cgcagacatc
     2821 ggcgagccac gcaggtgtgc aaaccctgat gtcgaaagac tcttcacctg gcgcgccttt
     2881 ggggccgacg atggcccgga tccgcaaaag gaaacggtcc ggggcatcag gcgcgtattg
     2941 ttccaggttg tcgccttcga ggctgtagag cgctttcacc tctgcggcga ccattggcta
     3001 gtgacctccg ccgatatcga gatagccgtt ggagtttcgg ggacatacgc aatcgccggt
     3061 cttcacgctt agcatagcta tcgcttgcct cgcccgcgtg gttgcggtcg gcgttccgca
     3121 tcatatcact ccggagattt tcggagttca gccctgctcc cggtcagtcg gcgaaatgcg
     3181 ccagagagaa cccgctgacg gactggttca tgagctcaag ttgtgacgag tacaggaagg
     3241 cgggatcacc gttcttcaga ctctcgcggt ggagccggcg gaagagcggg acgaggtcgg
     3301 ggtctcgcca gccgcgatcg acctcctggt cgaggatcac aaaggctttt tcggaactga
     3361 gcgccgcctt gtagggccgc acagtggtga gggcgtcgta tacgtcggcg acctgaagga
     3421 tacgggccgc cagagggatc tgctcgcctt tcagcccgtc ggggtagccg cttccatccc
     3481 agcgttcgtg atgactgcga ataatgggca gaaccggctt caggcttttc attccccggc
     3541 agatctcctc acccttcacg gggtgaagct tcatgacgga ccactcgtcc tcggtcagcc
     3601 ggccgttctt gtaaaggatg gaatccggaa cggcgacctt gccgatatcg tgcagaaagc
     3661 cgccgcgatg gagggca
//
LOCUS       DATIFQ010000401        44062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1474482, whole genome shotgun sequence.
ACCESSION   DATIFQ010000401 DATIFQ010000000
VERSION     DATIFQ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 44062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44062
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474482"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /gene="uvrA"
                     /locus_tag="VLE22_19440"
     CDS             <1..1861
                     /gene="uvrA"
                     /locus_tag="VLE22_19440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSB16634.1"
                     /translation="ELKRHGRGKVRLNVPFADLTDAEKRFLIEGEGRFRGIRGFFEKL
                     ETKKYKVHVRVFLSRYRGYAECPDCGGSRLRREALYVRVGDRTLADVSRMNIAEAHVF
                     FDSLSLTETEERIAGKILVEIRQRLKFLNDVGLDYLTLDRLSATLSGGEAQRIQLATC
                     LGSRLVGACYVLDEPSIGLHTRDTSRLIRILEELRDLGNTIVVVEHDADVMRSSDHIV
                     DLGPGAGELGGRIVFEGPFKALSSNGAGSLTGRYLRGELKTSHPRARRPVNSRKVVRF
                     LGARLHNLKNIDVEIPLGMMVAVTGVSGSGKSTLVHDVIYHALQSHFRKAGMAPTGEE
                     EEIPEVTEKVTCRRVEKADLLTDAVLVDQSPIGRTPRSNPVTYIKAFDIIRALFASTP
                     EADRRGYTPGHFSFNIPGGRCETCQGDGTVTVEMQFLADVELVCEECKGTRFKSGILE
                     VGYRGLNIHEVLQLTVKEALSVFADQPRLCARLKVLADVGLGYLRLGQSATTLSGGEA
                     QRVKLAAHLTQATCQGTLYIFDEPTTGLHFDDIAKLLDAFQKLIQNGGSILIIEHNME
                     VIRAADWIIDLGPEGGDRGGAIVAQGAPEQVAAAKASYTGRYLRNVLSEGKPG"
     gene            1958..3172
                     /locus_tag="VLE22_19445"
     CDS             1958..3172
                     /locus_tag="VLE22_19445"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB16635.1"
                     /translation="MWPAAVALLFWLQTVDYSAEGLKALEANKYEEAAQLFSKAVEAD
                     PNEYSAHFHLALSYSFLGKDAEAIPEYRKVLEIKPGLFEAELNLGIVLVRQKRAGEAL
                     PLLQSAAEKKPKDFRTVFYLAEATFAGGDFPKAEELYRSATEIDPKSPQAQLGLGRAM
                     LRQDRLAEGAGHFRQAVSLHASFRDALLELAALYEKQKQPSEAIAIYQQFPDDAAARE
                     RLGELLIETGKPAEAVQHLEWAVAKSPTPANRLALAMAYIRGGQTEKAVPFLEQVVVA
                     EPNNPELRMIYGRTLRDMKKYPDAAREFYRVAQAKPDSAEAWSELAGMLILLENYPQA
                     LAALDRVRALGAETAAHHFFRAIILDKGKLYEPALASYEKFLAMSDGKNPNEEFKARQ
                     RVRIIKKDLSRR"
     gene            3169..3630
                     /locus_tag="VLE22_19450"
     CDS             3169..3630
                     /locus_tag="VLE22_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16636.1"
                     /translation="MRRLRTILVAVTLLSCSRIAASADLSAVRAESNLQKRSDKALDY
                     AADVVKTLDKAYHTGGLARTQELLAEIRESVELAYTALKESGRNPRRHPKYFKRAELK
                     TRDLLKRLGDFEDRMAFDDREKLAPLRAYVQKVHDEILKGVLGAGEWQAKQ"
     gene            3612..4262
                     /locus_tag="VLE22_19455"
     CDS             3612..4262
                     /locus_tag="VLE22_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16637.1"
                     /translation="MAGKAMTARWLLLLCLALCLAAPIPAQDDFLTADEVDQLRLTQE
                     PNERIVLYLRFAQQRIDLAQDLVAKEKPGRSVLIHDALEQYTQIIDAIDTVADDALHR
                     KLSIQKGMEAVTESQKKMLAALRKVDESRPPDLPRYKFVLQQAIETTEDSLELSMQDL
                     NQRATEVEAREEREDKERESMMQPKDIEQKRAAEKKETEKKRKVPTLYKKGEQKKQ"
     gene            4328..6397
                     /locus_tag="VLE22_19460"
     CDS             4328..6397
                     /locus_tag="VLE22_19460"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HSB16638.1"
                     /translation="MPLTSRCIFCCVLIALSFCSLPAVAAGGLPDLRVPGAELVTVEE
                     ISSDRVKVTSPFWRMEFDLRGGGALDSIVFSHGSGRNVLTAPFRTCVDGWSDAEAPAV
                     EFRSTKEENVARLEFSGDLGASGRKKGPVAFRTVWIITARTIRADCTLRFESDLAVGH
                     VGVGSTALRGDLNEFGLRVGPGNNPRATSATYGKVSSAGSTWIDERHPPMYMVFFRRN
                     LEGFDITTGTDLATWESGLAGKGGAGRFQAGVSKGGAGIELVREPLSSISPVTVRKGE
                     YTFSYYLGLPRLVEKSDRGWRHIALGNHPWPSDELIRRWAENGVNIARLHNDYVADEN
                     FWHDGSWPPYDEKGMSELRRVIATCRRHKIRVVPYFSIHEFHPKSQGYAANESQWKRS
                     VDQAGTVFHNRTGQGEFGGPMCLQSGWLQRLKTNVERAYRELGFDGIYYDWSAVLPCN
                     NKGHDGNWHLGSDGMMDILAWTRRLIGSDGTLIIHVSGWLPSFPHENYADLLVNAEEA
                     SSGDRMMRKDNIALMTYLAEGVPRSPCPAYGKDRIVERNRNKLALMVAYGAFPWSGQD
                     GPVAEDTLRLFHTFQPYRLQDFRLYNADSGAVRTSWDDVHGAVYASPEQALVVLSNPE
                     AGPRKNVIWRVSPERLGYAPSAEVNIRDTASGRTSRVPWQALEDGSLATELDGFEYEL
                     FEMRPVR"
     gene            6454..7485
                     /locus_tag="VLE22_19465"
     CDS             6454..7485
                     /locus_tag="VLE22_19465"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HSB16639.1"
                     /translation="MGLLYQISGLVLSRGGRASHAQVKELQRDLRRLGYLRTGIDGDY
                     GKITESAVKALQFDLLKNTGSGPDGSAPVRIRDYNKGRVTAPTGVVDQQLVACIAEML
                     GDDRFAQVPDVPDPAAENRRVATKLSSLRSTEVPIPFLLGIFGQESGMRHFVEPTADN
                     DDNFVVVGLDRNSESQTPITSRGYGVGQYTLFHHPPTKAEVQDFVRDVSRNVVKAVRE
                     LREKLDHFVNGNTPGTRADDRQMEFGRRPLRICKYAPEDPRFLTDCRQCMMDAGSTDI
                     RAGVTRFYEGAQPFYEPTQYHPEREYKGVPIRRNIGCDWPYAVRRYNGGGLDSYHYQT
                     KVLLRVLHG"
     gene            7516..7971
                     /locus_tag="VLE22_19470"
     CDS             7516..7971
                     /locus_tag="VLE22_19470"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HSB16640.1"
                     /translation="MRAIPSWDEYYLSICKIVATRSKDPNTQIGSVIVGPAHEIRTTG
                     YNSFPRGIRDDVPERLERPTKYLWIEHAERNAICNAARCGTALDGCTLFVEIMPCMDC
                     ARAIVQAGIRQVVISGERMAQYSSDYYNQHFGMVEVLFAEAGVAVRRVP"
     gene            8060..8497
                     /locus_tag="VLE22_19475"
     CDS             8060..8497
                     /locus_tag="VLE22_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16641.1"
                     /translation="MPVTVKPVTLWRKEIENKPGTLAVALEPLAMAGADLQVIMGYRF
                     PGRSDAAALELFPVKGKKATAAAQAGGLSASTIPTLLVQGDNKAGLGYTIATSIAGAG
                     INIGFVIAQVVGRKYSAVIGFDTSDDAKMAAILIKKAAASKKK"
     gene            8568..9374
                     /locus_tag="VLE22_19480"
     CDS             8568..9374
                     /locus_tag="VLE22_19480"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB16642.1"
                     /translation="MRFPGSLFLHNLIERRALLFQLVRRDFEQRFVGSAAGWLWGVIH
                     PLVLLLSWTFVFDWCLNVRLGADAATQNYPLFLFSGFLPWLLFQDTVQRSSGSLLEHA
                     NLITKTVFPSEVVPLSIFLSSLVHHLIGLALVVAAVALFLNHFGVMILFLPLYMALLG
                     LFAVGIGWIVAALQVYLRDTAHVLSVVLTLWFWLTPIFITESRYPPPVRFLLAINPLA
                     HIVRAYRERILTYRLPDVEELAVVAAFSAITFFVGGLFFRHLKRGFADVL"
     gene            9391..10767
                     /locus_tag="VLE22_19485"
     CDS             9391..10767
                     /locus_tag="VLE22_19485"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB16643.1"
                     /translation="MSLSRRSFLLSSAAPALAQRKPQPARPNIVLIVADDLAAWMLGC
                     YGNKEIRTPNIDLLARSGTRFLNNFVCTPICSASRATLFTGRTPRQHGIHDFLTEKPI
                     EKPPQGQAAPPPSFRDEVMISDVLAGNGYQCGYAGKWHMGDDQKPQHGIKHWYTMLGG
                     SSPYQNPRMSLNGEIVQEQGYLADLITRNATGFIEQQSPANPFLLVASYFNPHVPYDG
                     HPPKYYDIYAKTTFETVGWEPPAPNALREKEYLTDIIGNLRRCAAAVTALDDQVATLI
                     SALDRKGLRDNTLVVFTGDNGFLLGRHGLWSKGHASDPINMYEEVVQTPMIWNWPGKI
                     PVEAVRPELVSFYDVMPSVCDVAGVATPAGRNLCGRSYAPLAMNRPLPRKQPWQNLVF
                     GHFRNTEMARDKRFKLVIRDGGEGPGELYDLVADPREKVNRYNAQEFVTVRHRLSAEL
                     EVWRKKYA"
     gene            10827..11972
                     /locus_tag="VLE22_19490"
     CDS             10827..11972
                     /locus_tag="VLE22_19490"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSB16644.1"
                     /translation="MPKSATVLLLCLAGCGTALLCAAALSFERVEIDAAPPQNPWIKV
                     AGDLDGDGKADVVIGGSKGPLVWYRNPSWTKSVIAAGGYDSVDAEAADIDGDGDLDLV
                     VGGLLWYENPRPAGDPGKSPWRAHRIASHHTHDVEVADLDRDGKLDIVVRGQSGFGAN
                     EGHRIFVFRQNARDSWASREIECPPGEGLKLADVDADGDADIVVAGRWFENTGAALAG
                     GWHERIYTSAWTYEDSAVATGDLNGDGRLDILLTPAEYKGGSYRVAWYEAPANPMSGS
                     WAEHVVAAPVETVIHAAAIADMDGDKAPDLVTAAMHQGAAPQEVTVFLNRGSGLRWEK
                     SVVSTRGSHNILLVDLDGDGRIDILGANHAGPYSPVEWWRNTSRLTR"
     gene            12581..12829
                     /locus_tag="VLE22_19495"
     CDS             12581..12829
                     /locus_tag="VLE22_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16645.1"
                     /translation="MPSLQLRDVPPELYHRIADLARREHRSLTQQAVILLARALDVSE
                     DPVRRRRRLLDAVSGRPLVASLDGVPPPEALVWEDRKG"
     gene            complement(12890..13426)
                     /locus_tag="VLE22_19500"
     CDS             complement(12890..13426)
                     /locus_tag="VLE22_19500"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSB16646.1"
                     /translation="MERLPFQVTGAAEPYDGIVRMPVAVLLDNIRSMYNVGAFFRTAD
                     AAAVEKLYLSGITAHPPKRALSKTALGAEETVPWEHSWEPVSLAERLRTDGYEIAAIE
                     TSMHAVDLFDWAPRFPVCVVFGHETEGIRPELSALCDTHVRIPMLGRKHSLNVATAGG
                     IVLYELLRKYRRLREQGR"
     gene            13484..14983
                     /gene="hutH"
                     /locus_tag="VLE22_19505"
     CDS             13484..14983
                     /gene="hutH"
                     /locus_tag="VLE22_19505"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HSB16647.1"
                     /translation="MVSLDGESLSLEQVSAVAMKSEPVSLAASSLEKMRRSRAVVEHL
                     AEGEEPVYGVNTGVGRLADVRIAKEDLEQLQRNIVRSHACGVGDPLSREEVRAMMLIR
                     ANVLAKGFSGIRPVVAERLCDLLNHDIIPVVPSRGSVGASGDLAPLAHMALVLIGEGE
                     AEFEGARWQGGEALRRAGSPPLTLASKEGVSLLNGTQAMLAVGCLQLHAAEILADAAD
                     LACAMTIDALRGTPRAFDPRLQQVRPHPGQLRSAANLARLLDGSEIRASHISCRRVQD
                     AYSLRCAPQVHGAARDALSEARRIFAIELNSATDNPLVFEDEILSGGNFHGQPLALVL
                     DYMAIAMTALAGISERRIDRLVNPERNEGLPPFLAAHAGLESGFMMAQVTAAALAAEN
                     RTLAAPASPGSITTSGDKEDFVSMGMTAALKLKQVVTNTRTVLAIEMMTGARALDCLA
                     PLRSSPMIEKLRMCLREITPPYETDRPLSADIERIALCISHGGPSDLLA"
     gene            complement(15007..15792)
                     /locus_tag="VLE22_19510"
     CDS             complement(15007..15792)
                     /locus_tag="VLE22_19510"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB16648.1"
                     /translation="MDRAGSVEENLVEFFRHFARARSSGEVREMPGVSIASCGASIHT
                     FNGAFLSEPVAEGAGLLERRILTASVHYAARGLRWAFWAAEHKLPAAIRKKSRAVFQA
                     HGLSLAYENPGMAADRLLPPSRRLPNLDFRRVEKEPERLDFCFVNAAAFRMPFEWCRE
                     LYNLEPLWNDQFTGWVGYVNGRPVTAAATLAVSGAVGVYCVATLPEEGRKGYAEAVVR
                     HALKKVREEPGFERTILQSSAEGLSLYKQMGYEVVTRFQVYST"
     gene            complement(15801..18224)
                     /gene="priA"
                     /locus_tag="VLE22_19515"
     CDS             complement(15801..18224)
                     /gene="priA"
                     /locus_tag="VLE22_19515"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HSB16649.1"
                     /translation="MPEADYRYCDVSVPVPLDQAFTYELPETLRHRTKPGCRLIVPFG
                     SRKLTGVVLRCHDGPPAMEVRRALRLLDSEPVLDDELLALGRWIGAYYCAPLGEVLRI
                     MTPLAAEVRSSKVYSLTDAGRDATRQLLLDDSPDDPATEVLRVLEARPLTAASIGKKV
                     PLAEKMLRSLERKGLVYAEEVRFERDPLRAPSPKLRLELLRHPDGTKVPKAERELLAY
                     LELHPGSHNLREVEEAVKNASGAARSLARKGFASLTPEPLALASLPGRPPHVLNPHQE
                     AAFNAVRRSIEAREFRTYLLQGVTGSGKTEVYLRAIDTALALGRGALLLVPEIALTPA
                     MAGQFFHRFGDRVAILHSAFTDAERAEQWRRIRSGAAPVVVGTRSGVFAPVRNLGLIV
                     VDEEHDQSYKQEEAPRYNGRDVAVVRGKNAGACVILGSATPSLESRYNTERGKYSLLE
                     LPERIEQRPLPRVELIDMRLEFLETRTQSTFSRRLVEEVQQRLENGEQTMLLLNRRGF
                     SSFLACRKCGERLECVNCSVTMTFHRRERRMLCHYCNYAIKVPNACPKCQSEHIYFLG
                     VGSERVEEELHRAFPRARIARMDRDTVSGKRQFETILQGFRDGNFDILVGTQMIAKGH
                     DIPNVTLVGVVNADIGLGMPDFRAAERTFQLLTQVAGRAGRGDTPGIVLVQTINPDHY
                     AVRFAAAQDYRLFYEKEIQFRRSMRYPPFSALANLLVRSEKQEEAMRMSGELGRLLDP
                     PPEGLRILGPAEAPVARLRNEYRYQLLIKAANRKVLNETLRTVRDFALESKWNATALV
                     IDVDPLTLM"
     gene            18348..19859
                     /locus_tag="VLE22_19520"
     CDS             18348..19859
                     /locus_tag="VLE22_19520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB16650.1"
                     /translation="MTDAAGDRLDSWKEIAAYLKRGTRTVQRWEAEEGLPVRRLQHDK
                     LGSVYAYKKELDAWWASRRPAEERESLVGAESGASIAVLPFTDMSQEKDQGYFCEGMA
                     EEIINALGRPNGLRVASRISSFQFRSSSAGTREIGRKLRVGTILEGGVRKSGNRLRIT
                     VQLTNAEDGYQLWSARYDREMSDVFAIQDEIAENVVQALKVTLSPGERAAREKAPTSD
                     VQAYDLYLRGRHYFYHYGTRDIEFARNLFSRAIELDPHYVLAWAGLADCWSYLFLYAQ
                     RSDENRRQAEEASRRAVELDPESAQAQASRAVALSLSERNEEAEAAFEKAIRMDPALF
                     EAHYFYARHCFVHGRVEEAVALYERAMQVRPEDYQVPLLMAQIYDDLGRWDVAAETRC
                     RGVEKAEKHLEWNPDDARALYMAANGLVALGERERGLELAERALAMRPDEPMTLYNVG
                     CIYSLLGRVEPAVHCLEKAVGNGLGQKGWFDHDSNLDPIRTHPRFARLMKKLA"
     gene            complement(19911..20762)
                     /locus_tag="VLE22_19525"
     CDS             complement(19911..20762)
                     /locus_tag="VLE22_19525"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB16651.1"
                     /translation="MLSRRGALRILAVPMIPGGDEGPNMFGDAEAYERFMGRWSHLVA
                     PLLVDFSGLPDEGRALDVGSGTGSLAFAIAKRKERSHVLGIDPSEAYVAYANSRNPFP
                     GRVRFQVGNAQQLQFPDAAFAATLSLLVFNFIPDREKALREVRRVTTAGGRISAAVWD
                     YGAGMRMLRFFWDAAVSIDARAERFDEKHMPLCRASELSELWKQGGLKSVHEQPLDIT
                     MRFESFADYWDAFLLGQGPAGAYARTLGRDRLEALRGAVKRRLSLSADDRPFTLPARA
                     WAVRGTV"
     gene            20874..21836
                     /gene="fliM"
                     /locus_tag="VLE22_19530"
     CDS             20874..21836
                     /gene="fliM"
                     /locus_tag="VLE22_19530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HSB16652.1"
                     /translation="MNRQLSQQEIDAVFENLGKGEGGRTGVLKTAPFDFRRPDRIPKS
                     QLRALRLLHENFVRSLASSLSAYLRTYLTANLVSVEQLSYAEFVETLSSPTCLVSLGL
                     RPYDGHAVLELGPSLTFQILEVILGGKGKGPPAQRREITEIERSLLDGLLHIILSELK
                     QAWESVTAIEFTVESLETEPQFLQVPDPGEAFVAVTIELKVGEVAGLMNLALPSLIIK
                     MMRAKFDHHGSLRKMQSSGEEQARMLRLMKPAKLSLDARLRGPSMLVRDLLSLAEGDV
                     VPLDYAVQRPLELLVNGRLRFRGQLVSAGNKKAFVIGGREEQQR"
     gene            21833..22033
                     /locus_tag="VLE22_19535"
     CDS             21833..22033
                     /locus_tag="VLE22_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16653.1"
                     /translation="MRFGCLPWAGGRPAEVFEKPDAGVGTYHARIAGGPHAFQDAFQQ
                     IPAFVFVAGDRGTGDAGVADAV"
     gene            22237..22482
                     /locus_tag="VLE22_19540"
     CDS             22237..22482
                     /locus_tag="VLE22_19540"
                     /inference="COORDINATES: protein motif:HMM:NF018043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HSB16654.1"
                     /translation="MIRLTRLNHLPLVLNSDLIEHIEVTPDTVISLTTGQKIMVLETA
                     EEVVGRVIEFRRAMFHGLLECPGRLTPAGEPAGVEGF"
     gene            22489..23349
                     /locus_tag="VLE22_19545"
     CDS             22489..23349
                     /locus_tag="VLE22_19545"
                     /inference="COORDINATES: protein motif:HMM:NF006583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HSB16655.1"
                     /translation="MAGVAEGKSKRGRTKPDLASWGGLLLASTGIIGGLLLEGGTIQD
                     ISQVTAGMIVLGGTLGAVMVTTPLSVLLSAARKLKLVFFEQAQTPDAVIEEIITYATK
                     ARKNGIVSLEQEAEAVRDPFLRKALNLAVDGMDLKELRKMMELEIGLEEQRAEAEAKV
                     YEAAGGYAPTIGIIGAVLGLIQVMKHLENINEVGKGIAVAFVATVYGVGSANIFFIPA
                     GHKIRARMHGVLQTKELMIEGVTGIVEGLNPKLIRIRLEAYTRRDAKTKPAKGSKEAK
                     PAGEPAAISS"
     gene            23365..24171
                     /locus_tag="VLE22_19550"
     CDS             23365..24171
                     /locus_tag="VLE22_19550"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HSB16656.1"
                     /translation="MARKKQAQHDNHDRWLVSYADFITLLFAFFVMMFASSQTDRGKA
                     QQVSESVRKALEETHAVSRIVAILGGSAKDAGKGDSMAAPKDPGAPNETVVDLLPSLE
                     FLSRELSAEIRAGKMQINLEPRGLVVSLPQATFFPSGQDTIDPATYAVIEKVSAAIRK
                     IPNPVRLEGHTDSVPIHNVRFRSNWELSAARSIAMLELLAVRYEIPRERLAVAGYADT
                     VPAEPNDSEEGRARNRRVDIVVLSRIGLMREPEPGARTEPPPSAKGPKGA"
     gene            24252..24710
                     /gene="fliW"
                     /locus_tag="VLE22_19555"
     CDS             24252..24710
                     /gene="fliW"
                     /locus_tag="VLE22_19555"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HSB16657.1"
                     /translation="MPVSHTRYFGTIDYRDESVVRFPHGLPGFAEETRFLLIDQPVNE
                     PLVFLQSLSRPDLCFLALPVLAVCPGYQLSIPAEDLATLGLPEQPQPEIGAELSCLAI
                     VSLAEDRPPVANLLAPVLIHWKNRRAVQAIQAEFGYSYEHPLFAEAAPCS"
     gene            24707..24931
                     /locus_tag="VLE22_19560"
     CDS             24707..24931
                     /locus_tag="VLE22_19560"
                     /inference="COORDINATES: protein motif:HMM:NF014638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HSB16658.1"
                     /translation="MIRRRAGESILIGDDIRIEIIETGAARVKLGITAPRDVPVLRNE
                     IRLAGEANQAAAREVTAAALSRLVDRLRRT"
     gene            25124..26632
                     /locus_tag="VLE22_19565"
     CDS             25124..26632
                     /locus_tag="VLE22_19565"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HSB16659.1"
                     /translation="MAVSIQTNVNSIVAQENLRVNGEFQSRTIQRLTSGFRINSSGDD
                     AAGLAVANKFRSDIAELTQGVRNANDAISVLQLVDGGLNNVSKLLDRLKTLASQSASG
                     TFTGSRTTLNNEYQALLTEIDRQASNIGLVGGGANNKVLDIYIGGGSTQANAQVSIDL
                     SGTANQTDSAGLGLANTAISGGGTALTGNSVRLDNSALSFLNGSDTQAFTFRIYNGSG
                     TENVVVTVAGSASGISGTEVLNQLNSALSTYGISASTVGSGADVGELQFGGSRAFSVS
                     VAAPAGAGQAAVTAGSTAVNTGNYHYDVAAADFVAVSAGSEVVTFANANGTANVTFST
                     TSGNTVANSIAAINAQTASLGIYAVADAAGTGFSIQSSSSFTLVKTQAAGTGVVITGA
                     VGAKTVTAPAAGASATGNALSAITSLTSAVTTLGLVQGRVGTGQNKMFYAIQLAQSQI
                     SSFSAAESRIRDADIAAEAANLTKAQVLEQASLAAMAQANSSPQAVLALLRA"
     gene            26715..28067
                     /gene="fliD"
                     /locus_tag="VLE22_19570"
     CDS             26715..28067
                     /gene="fliD"
                     /locus_tag="VLE22_19570"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HSB16660.1"
                     /translation="MNSTSAIFNGTSRYSADFQQIIDRAVAIASLPMMQMESAKTALD
                     DQAKALESLDTRFAAVDSAIRSLESAAANNSYVLSNSDGGVVAASVSSGALTGVYTVE
                     VVEAGSYTSSMSKDGLTAVADPSAESISLASTFTLTVAGETYEITPSGDTLTALAAAI
                     NAETEASVEATIVNIGGPSAPDYRLSLRSARLGAVSIQLNDGSADLVDTLASGTPASY
                     RVNGQPSTAVSTDSRSVTVAPGLTVTLLKAGTANLTVSRNTAGISNALASFAGAYNAA
                     VDEVDLHRGKDAGALKGNSVLFTLSQTLREIAGYSTGTDGIASLTSLGLAFDDQGKLS
                     FDATAFSNAVAGNLSGLLSFLGSASESGFLQSATSAMDAVVGTTDGIVKAAIGSLQTQ
                     IAGQAGRISSEQDRIDQIEQGLRERMAAADALIAALERQVGYFTSLFESMRLARESRR
                     "
     gene            28064..28369
                     /locus_tag="VLE22_19575"
     CDS             28064..28369
                     /locus_tag="VLE22_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16661.1"
                     /translation="MTEAGVVEARFREAVARGDWAEARRCLPEYGREIEERLRNLPPG
                     EDAAALLGRAVKLLEQVRRAAVSERAQAGTELERLAAAVSYRRAGHEGFRATLELEG"
     gene            28478..28816
                     /locus_tag="VLE22_19580"
     CDS             28478..28816
                     /locus_tag="VLE22_19580"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HSB16662.1"
                     /translation="MLDRLGGRLEQYMNLLSARQRQVASNIANADTPGYKTRDINFQA
                     EFRDLMENGSPRAVEVAGLQAKNDGNNVSLDREARLLAENALRFSVATSLARAEIRAV
                     KSAIQEGKGV"
     gene            28813..29247
                     /gene="flgC"
                     /locus_tag="VLE22_19585"
     CDS             28813..29247
                     /gene="flgC"
                     /locus_tag="VLE22_19585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HSB16663.1"
                     /translation="MSLFSLLSVSASGMAAQRTRAELLVENLANAETTRTPEGGPYRR
                     KDAVFAVDGAPSPFASVFQSVSGAPDGVKVEEIVVDTREPERRYLPGHPDADGEGYVA
                     FPRVNAAEDMVDLMGASRGYHANVSAMSAVKEMIHRSIDLLR"
     gene            29261..29557
                     /gene="fliE"
                     /locus_tag="VLE22_19590"
     CDS             29261..29557
                     /gene="fliE"
                     /locus_tag="VLE22_19590"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HSB16664.1"
                     /translation="MVNSISPIRNVSLPELAAPPGKEGASGAFRSLLEGAIVPVENAR
                     GLAADSVEKFLAGEGEELHSTVLAVQRAEMALDLFLQVRNKVVQAYQEIMRMQV"
     gene            29583..31223
                     /gene="fliF"
                     /locus_tag="VLE22_19595"
     CDS             29583..31223
                     /gene="fliF"
                     /locus_tag="VLE22_19595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HSB16665.1"
                     /translation="MSQWKKILASFSTRQRVAILLAVAVAGAGLWALTRWRRETDFRP
                     LYTALEADDAAAMVSRLKETGVEYRLSEDGRTILAPSAKVAEARLSMAAAGLPKTGRI
                     GFELFDGTKFLATEFAEHVNYARALEGELERSVMYLAAVERARIHLTFPKDSVFLEAR
                     QPAKASVILKVRPGARLTPQNVVAVSHLVASAVEGLTPDAVSVLDMQGNLLSRPRRML
                     TADGMGASDGMLEFRQSVEKDLLAKIHATLEPLGGPEKFRASVSVDCDFSSGEQSEET
                     FDPARSVMVTSQKTEDVSGGAGSSGVPGTASNLPRPSSRPGSSQTGLTRRTENITYQS
                     SRVVRRMRLPLGNTQRMSVAVIVDQAVRWEGTGAKARKVMEPPTPERMKSIRDLVAGA
                     IGFNAARGDRLIVESLPFESTLQLEPPEGAPAPSPASPLSAWLEILRKAGAAAILLLA
                     LSAAVVLARRKRRRKVQISSPRELSATPGEKPDGKSIAGVVEEAKRIEQASGALLESP
                     PAPTKKADLLAGRLRDVIKADPSGLAQVMRTWLAEEEE"
     gene            31236..32267
                     /gene="fliG"
                     /locus_tag="VLE22_19600"
     CDS             31236..32267
                     /gene="fliG"
                     /locus_tag="VLE22_19600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HSB16666.1"
                     /translation="MILNSPKAAPGIPGAQKAAILLMMLGEETGAELMRQLSEDDVQL
                     ISREVARLGTIAPDQAEAVLDEFHQMAVARDYVVKGGFDHARRMLVSAFGPEAAEKIL
                     YRLTRTLHADMTDFAALQKADPQQLAKFVASEHPQTVALIVSQLSSTQAASLLMSLPP
                     EMRSDVTLRMASLDQISPEIINKIAGIVHQRLKGLGQISRQSYGGVRAVAELLNRLDS
                     SVTKEILESIHQQDSEMTEKIRHLMFVFEDLLLLDQSGVKEILAKVDRKLLTMALKGT
                     SESLRNHVVQCMSQRGREMLLEDMDALGPVKIKEVEAAQQAIIAVVRQLESEGVLSLK
                     ETAGEQYVV"
     gene            32257..32880
                     /locus_tag="VLE22_19605"
     CDS             32257..32880
                     /locus_tag="VLE22_19605"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HSB16667.1"
                     /translation="MSSRILREPGPGRAEPVVWPSLTAPQPAPTPEPSTEKAPDPAEE
                     LDRRLQEAHRSGYREGEAAGRAQLAPVLEQLAGSIESLASLRPLLRKKAESDTVQLAL
                     AVARRILRRELTVDPEALEGLVRAAFEKLQGQEICRVRLWPGHETVVREWLDRLGAPQ
                     TIEIIADRSRHPGDVVFETARGALDASVESQLAEIEYGLADRLRRRT"
     gene            32877..34205
                     /locus_tag="VLE22_19610"
     CDS             32877..34205
                     /locus_tag="VLE22_19610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HSB16668.1"
                     /translation="MSVPAQIDLSPYLRDVDHVEPLRWCGEVVELVGLLVVSRGPVAA
                     VGDLCEIRTVSGSRIRTQVIGFRDGHVLSMPLEETGGLQLGDPVVARGGEASVGVGQG
                     LLGRVLDGFGRPMDGGGEVDAEAEYGLYAQPPNALEREHIDQPLVTGIRTIDSLLTCG
                     KGQRIGIFGGSGVGKSTLLGTMSRHNFAEVSVIALIGERNREVRAFLEHELGEEGRRR
                     SVVVVATSDQPAPLRVRACFVALAVSEYFRDRGADVLLVMDSVTRLAMAQREIGLAAG
                     EPPSQKGYTPSVFNLLPKIFERAGRFRRGSITGFFTVLVEGDDFNEPLCDAVRATLDG
                     HIVLSRQLGTAGHYPAIEVLNSVSRLTSSITSREHAEWARKVREAMAAYQQAEDLIQL
                     GAYVGGTNPKLDTILEMREELMEFLRQDASVKTPASETLARLEALASKLP"
     gene            34221..34688
                     /locus_tag="VLE22_19615"
     CDS             34221..34688
                     /locus_tag="VLE22_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16669.1"
                     /translation="MKAFYFRLERVLEWRRTEFELAEHRLREAAAALGRLDEESRRLS
                     AGREAAERALRTADSVDGADLGAHSSYLRRLRRHADALAAHRVQLDRELAARQQRLIE
                     ARRQLRIIEKYRERRLVAWQAQLGREIEAFAGESYLARWNSRQSGRKIPPAGR"
     gene            34696..35043
                     /locus_tag="VLE22_19620"
     CDS             34696..35043
                     /locus_tag="VLE22_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16670.1"
                     /translation="MSEFLRNAESILDGAAAALASGGQPSEMVILVGRDGVIRIVAEP
                     DWPLDALEAYHSARMAYRVSGRDGRVRVEGRCGGASCMIVADPPASVARRLLAGHAWA
                     PNVPLACLPPAAS"
     gene            35153..35434
                     /locus_tag="VLE22_19625"
     CDS             35153..35434
                     /locus_tag="VLE22_19625"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HSB16671.1"
                     /translation="MDITPMHRPGSALSPAPAPVPAETAVENREIIQAVKALNAAELF
                     GQNSELTFLLDRRTQRPVIRLVDRKTKDVIRQIPPEHVLRMARDLAHAE"
     gene            35499..35969
                     /gene="fliS"
                     /locus_tag="VLE22_19630"
     CDS             35499..35969
                     /gene="fliS"
                     /locus_tag="VLE22_19630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HSB16672.1"
                     /translation="MPASAHDAYLESTILSADPVELVRILYRTALDAVREARVHLASG
                     DITARGKTVSRALDVINELNGSLDHQTGGALSRQLSELYDYMQRRLIEANYRQETEAL
                     DEVSALLATLLEAWQAVGTSAQPEESRFEADLRQMASLPEFQAEMEYAPQAWSA"
     gene            complement(35999..36277)
                     /locus_tag="VLE22_19635"
     CDS             complement(35999..36277)
                     /locus_tag="VLE22_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16673.1"
                     /translation="MTVGGIRPSPNAPPEGDSRPERIEDAARQFEALLIAQLLKSMRE
                     AGPGWLGTDDDSTSSSAMELAEEQFAQSLAAQGGLGLARIVASGLRKD"
     gene            complement(36277..37380)
                     /locus_tag="VLE22_19640"
     CDS             complement(36277..37380)
                     /locus_tag="VLE22_19640"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HSB16674.1"
                     /translation="MRRLFGISAWLLAGMLGAGPGETRLKELATLEGVRDNQLMGYGL
                     VVGLAGTGDRRQTVFSAQSLTNVLERMGVSVSPEAIRVNNVAAVMVTATLPAFAQTGT
                     RIDITAAAIGDASNLQGGLLLLTSLRGVDGQVYAVAQGPVVTGGFVAGRQGTNQTLNH
                     PTVGRVPNGAIVERAAPSLAPKGSVRLQLRQPDFTTAARVAAAVNKSFGSAAKPTAQA
                     ENAALIRVEIPADYAARPTEFVAELERLSVEADRPSRVVVNERTGTIVMGKEVRISPV
                     AIMHGNLTIEIETSFAVSQPAPLSSGTTEVVPQVGVGVKEEKARNLVLKQGATVEELV
                     RALSSIGSTPRDIIAILQSLRGAGALEAELEVI"
     gene            complement(37380..38078)
                     /locus_tag="VLE22_19645"
     CDS             complement(37380..38078)
                     /locus_tag="VLE22_19645"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HSB16675.1"
                     /translation="MIRRNCFCLLVLAAVALPALPAKKDRSPAPSPLDRYVEEAMARD
                     GPGRQSASPGSVWSSSAKLADPARDVRAGAVDDVVTILVMERASAVAKGSTKSARVSS
                     NRNSISALGGITRAAGPLANLTNLDGESQLSGEGSTSRETLLSTTLAARVTHVLPNGL
                     LVVAGTKDLQVNSEQQTVTVRGVVRPEDLNPGNIVRSDRISQLEVRINGKGVVGDAVR
                     RPFFLYRLLLGLLP"
     gene            complement(38075..38956)
                     /gene="flgA"
                     /locus_tag="VLE22_19650"
     CDS             complement(38075..38956)
                     /gene="flgA"
                     /locus_tag="VLE22_19650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HSB16676.1"
                     /translation="MTLLAALLVSGCLAVEGEQIVAADLAGAEPAFARLPAETPLGFA
                     PAPGARRILRRAELARLGRRHSVDVDPSKEICIERLLEPLRSDRLLDAMTRALGRPEA
                     KIELVDFSRYPAPKGEVEFPVSGLSRSSDPETPAVWRGSVLHDGKRRFRIWARVRISI
                     PAKRLIATEDLPVGRPIGAAQVKQETYSADPRPAIYAAQPEQAVGRTPRRRIAAGQPV
                     PLGALEAPREVAGGEMVRVEAASGAARIEIHGRAETSGRKGDTVLVRNPESGRRFRAR
                     VIGRGLVAAGDERKPTR"
     gene            complement(38953..39744)
                     /gene="flgG"
                     /locus_tag="VLE22_19655"
     CDS             complement(38953..39744)
                     /gene="flgG"
                     /locus_tag="VLE22_19655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HSB16677.1"
                     /translation="MIRALFSAASGMTAQQLNVDNIAHNLANANTIGYKVRRAQFQDL
                     LYQNLVTPGASAGQQTVVPTGLQLGLGTRAASNEILFSQGNFSATENPLDVVVQGRGF
                     FQVRLPSGEIAYTRAGSFHLDRDGNLVTADGDPLEPQITIPPDAQAISIAPDGTVSFT
                     QPGQTAIQLAGQIQLANFQNPAGLNSIGKNLYLPTDASGDPTIGNPGGQEGIGTLLQG
                     YLEQSNVSVVEEFINLIVSQRAYEANSKVVKAADDMYQQINNLTR"
     gene            complement(39774..40517)
                     /locus_tag="VLE22_19660"
     CDS             complement(39774..40517)
                     /locus_tag="VLE22_19660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HSB16678.1"
                     /translation="MDPLTISGASGLRARMESLEMLANNLANVSTDGFKTDREFYGLY
                     VSPESLDPAGGGITQTLPVIERHWTDFTQGVLHPTGNPFHVAISGRGFFAVNGPAGRL
                     YTRNGGFQLSATGVLSTAEGYPVRAVGSGTLRASSQSPIEVAPDGVVTQDGQVLGQLE
                     LVDFTDTGALAKQGRAYFRADDPRAQPVAATGAAVHQGKLEGSNVTGAEGAVRLIGVM
                     RQFEMLQKAVSIGGEMNRKVVEEVARVGS"
     gene            40746..42284
                     /locus_tag="VLE22_19665"
     CDS             40746..42284
                     /locus_tag="VLE22_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16679.1"
                     /translation="MTVSCFPLPLAGTILDVPAAVEGTATVTPQGANWIDPDPAAGAA
                     FGQLLGTVLETLSEEGIGTGTGTGPSIGRPPIPMRTETVPEPAESGDAKPETPETDPD
                     PAIAVAVPVSGVLPFAEPPLDAQPTVQPSRLAPEKPASDSWPFDVAHRVSLPDAAAGD
                     ESSDLPPASSGTRDVPAFSPAFTEDPGNADVPETAGVASLPTVRPPASSGPLNVEPPV
                     EWPPQKDRPPESSVPPIVTAPAVSFDSQPPTETGRTAAQRPASRPMITVRTAPAEEPP
                     VADAKPAFSMRIVGADRDQTADTEALLAPPEQPAGAKLRRATQVDTLTLLPEDPIAIA
                     AAPAARPGRPVQAETVRADQPEAEIDRTEPPEVAVRRMPETYRGAAARQISLRVAEGG
                     EERVEVRVAEQSGELRVDVRARNEGLAGRLRDGLPELVGRLQENGLEAEVWRPSGIDA
                     SKTPARRDELPVASQERNPGGERGSGWQQGEEGGRRRHQQDGQSQSEVFDPYFQLRNE
                     NGQE"
     gene            42291..42605
                     /locus_tag="VLE22_19670"
     CDS             42291..42605
                     /locus_tag="VLE22_19670"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HSB16680.1"
                     /translation="MTISSDPNIRTAADSGATGDGSASADNKDVSQVATKDAFLRLLV
                     AQIRNQNPLNPADGVQFLTQLAQFSELEQMITMRQEVEGLRDDIQAALNPAADTPAST
                     PA"
     gene            42624..43877
                     /locus_tag="VLE22_19675"
     CDS             42624..43877
                     /locus_tag="VLE22_19675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HSB16681.1"
                     /translation="MAFTAFSTALSSLSAHATAVDVVGNNLANLSTPGFKASTVVFHD
                     LVTQALGLGETQVGLGTGRPLTIRQFSQGALQSSGGNLDAAIQGDGFFIVRDYTGATL
                     LTRAGNFRVDEEGNLLTATGDQVQGWTESGGVVDTNAPIGTIVVPVGSLRAPSATTEF
                     SLNLNLNASAVVGEPSASFSTPIEVVDSIGATHVVTVNFTKTAANTWDYEVTIPGGEV
                     TAGTPGTPYSLGTETLTFDDHGLLATPAAPATIAVPVAGLASGAADLAIDWDLYDPDG
                     TSRLTQYSQTSAVAAVSQDGAPAAQLVKVGLGAGGTIMAQYSNGEQKVVGQIALAAVR
                     NPDSLIAVGNNNYIQTARTASPAIGMPETGGRGKIVSQSLEASTVDIAQEFTNLIVLQ
                     RGYQANARVVTAVDEISQETMNMKR"
BASE COUNT         8095 a        13941 c        14319 g         7707 t
ORIGIN      
        1 ggagctgaag cgccacggcc gcggcaaggt gcggttgaac gtgcctttcg ccgatctgac
       61 ggacgccgag aaacgtttct tgatcgaagg cgaagggcgt ttccgcggaa tccgcggttt
      121 cttcgaaaaa ctcgaaacta agaaatacaa ggttcatgtg cgggttttcc tgagccggta
      181 ccgcggctac gctgagtgtc cggactgcgg aggctcgcga ctgcgccgtg aggcgcttta
      241 cgttcgcgtc ggcgatcgca cgctggccga cgtctcacgg atgaatatcg cggaagccca
      301 cgtctttttc gacagcctga gtctgacgga aacggaagag cgcattgccg gcaagatcct
      361 ggtggagatc cggcagcggc tgaagtttct gaacgacgtg gggctcgact acctgacgct
      421 cgaccggctc tcggcgacgc tttccggcgg cgaggctcag cgcattcagc ttgcgacctg
      481 cctcggatcg cgcctcgtgg gcgcctgcta cgtgcttgac gagccgtcga tcgggctaca
      541 cacgcgcgac acgtcgagac tgatccgcat cctcgaggaa ttacgcgacc tcggaaacac
      601 gattgtggtc gtcgagcacg acgccgatgt catgcggtct tcggatcaca tcgtcgatct
      661 cggtcccggc gccggggagc ttggcgggag gatcgtgttt gagggacctt tcaaggcgct
      721 gtcctcgaat ggcgccgggt cgctcactgg gcgctacctg cggggcgagc tgaagacgtc
      781 gcacccccgc gcgcggcggc cggtcaactc ccggaaagta gtccggtttc ttggcgcgcg
      841 gcttcacaac ctgaaaaaca tcgacgtcga gattccgctc gggatgatgg tggcggtgac
      901 aggagtctcg ggctcgggca aatcgaccct cgtccacgac gtgatctacc acgctctcca
      961 gtcgcacttt cggaaggccg ggatggcgcc cacgggagag gaagaggaga tcccggaggt
     1021 taccgagaag gtcacttgcc ggcgcgtgga gaaggccgac ctgctgacag acgccgtgct
     1081 cgtggatcaa tcgcccatcg ggcgcacgcc gcgttccaac ccggttacct acatcaaagc
     1141 gttcgacatc atccgagcgc tgttcgcatc cactccggag gccgaccggc gcggttacac
     1201 ccccggacac ttctctttca acatccccgg cgggcggtgc gagacctgcc agggcgacgg
     1261 cacggtaacc gtcgagatgc agtttcttgc cgatgtcgag ctcgtttgcg aggagtgcaa
     1321 gggtacgcgc ttcaagagcg gcatcctcga ggtgggatat cgcggtctga acatccacga
     1381 agtgctccaa ctcacagtga aggaggcgct ctcggttttt gcagaccagc cgcgtctgtg
     1441 cgcacgcctg aaagttctcg ccgacgtcgg actcggttac ctgcgcctcg ggcagtccgc
     1501 cacgacgctc tcgggcggtg aagcgcagag ggtgaaactg gcggcccacc tgactcaggc
     1561 cacctgccag ggcacgctgt acatctttga cgagccgacc accggccttc atttcgacga
     1621 tattgccaag ctgctcgacg cgtttcagaa gctcattcaa aacggaggaa gcatcctgat
     1681 catcgagcac aacatggagg taatccgcgc ggccgactgg atcatcgatc tcggtccgga
     1741 gggcggcgac cgcggcggcg cgatcgttgc gcagggcgcc ccggaacagg ttgcggcggc
     1801 gaaagcttct tacacgggcc gctatctgcg caatgtgttg tccgagggaa aaccaggctg
     1861 acggtcgatt ggtttgaagg ctgatgaccg ccgcggcggc cggtggggaa ctgggccgtg
     1921 agcgaacgag ggctctggca caggatagac taaaagtatg tggcccgccg cggtcgcgtt
     1981 gctcttctgg ctccagacag tcgactattc ggccgagggc ctgaaggcgc ttgaagcgaa
     2041 caagtacgag gaagccgcgc agcttttctc gaaggcggtc gaggccgatc cgaacgagta
     2101 cagtgcacac ttccatctcg ccctttcgta tagctttctt gggaaggacg ccgaagcgat
     2161 acccgagtat cggaaggtcc tcgagatcaa gccaggattg ttcgaggccg agctgaacct
     2221 cggcatcgtt ctcgttcgcc agaagcgtgc gggcgaggcg cttccactcc ttcagtctgc
     2281 cgcggaaaag aaaccgaaag atttccgcac cgtgttctac ctcgcggagg cgacattcgc
     2341 tgggggggac ttcccaaagg ccgaggagct ctaccggtct gccacggaga ttgacccgaa
     2401 atcgccgcag gcgcaattgg ggctcggacg cgccatgcta cggcaggatc gtctcgcgga
     2461 gggagcggga cacttccggc aggcagtctc cctccatgcc tctttccggg atgcgctgct
     2521 cgagcttgcg gccctctacg aaaaacagaa gcagccgtcc gaggcgatcg cgatttacca
     2581 gcagtttccc gacgacgcgg cagcccgcga acggcttggc gagctgctga tcgagactgg
     2641 caagcccgca gaggcggtac agcacctgga atgggcggtc gcgaagtcgc ccacgccggc
     2701 caaccggctg gcgctggcga tggcttacat ccgtggaggc cagaccgaga aggcagtgcc
     2761 gtttctggaa caggtggtgg tggctgagcc gaataatccc gaactccgga tgatctatgg
     2821 ccgcacgctg cgggacatga agaaataccc ggacgccgcc cgcgagtttt atcgcgtcgc
     2881 ccaggcgaag ccggattcgg cggaagcctg gagcgaactg gccggcatgc tcatccttct
     2941 ggagaactac ccgcaggcgc tggcggcgct cgaccgcgtt cgtgcgctcg gcgccgaaac
     3001 ggccgcccat cattttttcc gcgccatcat tcttgataag ggcaagctgt acgaaccggc
     3061 gcttgcgagc tacgagaaat ttctcgcgat gagtgacgga aagaacccaa acgaggagtt
     3121 caaagcccgt cagcgtgtac ggatcatcaa gaaagacttg agccggcgat gagacgactg
     3181 cgcaccattc ttgtggctgt gacgctcttg tcctgtagca ggatagcggc cagtgccgac
     3241 ctctcggccg tacgggcgga atcgaatctg cagaagcggt cggacaaggc gctcgactac
     3301 gcggcagacg tggtcaagac cctcgataag gcttaccaca ccggcggctt ggccaggaca
     3361 caggagttgc tagcggagat ccgcgaatct gtggaactcg cctacacggc ccttaaagaa
     3421 agtggccgga accctcgcag acatccgaaa tatttcaagc gcgcggaact gaagacccga
     3481 gatctgctga agagactcgg cgatttcgaa gaccgaatgg cattcgacga ccgcgagaag
     3541 ctggcgccgt tgagagccta cgtgcaaaag gtccatgatg agatactgaa aggcgtactg
     3601 ggggccggag aatggcaggc aaagcaatga ccgcgcgatg gctgcttctc ctctgtctcg
     3661 ccctctgtct cgccgcgccg attcccgctc aggacgactt cctgactgcc gacgaagtgg
     3721 accagcttcg cctcacgcag gaaccgaacg agcggatcgt gctctacctg cgattcgcgc
     3781 agcagcgcat cgacctcgcc caggaccttg tcgcgaagga gaagcccggc cgctcggttc
     3841 tgattcacga cgcgctcgag cagtacaccc aaatcatcga cgccatcgac accgtcgcgg
     3901 acgatgcgct ccaccgcaag ttatcgatcc agaagggcat ggaggcggtt accgagagcc
     3961 agaagaagat gctcgccgct ctcaggaagg tcgatgaaag ccgtccgccg gatctcccac
     4021 gctacaagtt cgtcttacag caggcgattg agacgacgga agacagcctc gagctctcga
     4081 tgcaggacct caaccagcgc gcgaccgagg tcgaggcccg cgaggagcgc gaagacaaag
     4141 agcgcgagag catgatgcag ccgaaggaca tcgagcagaa gcgcgcggcc gaaaaaaagg
     4201 aaaccgagaa gaagagaaaa gtacccacgc tgtacaagaa aggcgagcag aagaagcagt
     4261 agtgccccac ccgttggccc cttcttgggt gtaatctgtg atcgacattt ctgtgtcgag
     4321 accgatcatg cccttaactt ctcgctgtat tttctgctgc gttctgatcg ctctgtcatt
     4381 ctgctccctt ccggcggttg ccgctggcgg cctccctgat cttcgcgtcc cgggcgcgga
     4441 gcttgtcacg gtcgaggaga tttccagcga ccgtgtcaaa gtgaccagcc cgttctggcg
     4501 gatggaattc gatctccgtg gcggcggtgc actcgactcc attgtgtttt cacacgggtc
     4561 aggcagaaac gttctgaccg ccccctttcg cacttgcgtc gacggatggt ccgacgctga
     4621 ggcgcctgcc gtcgaattcc gctcgacgaa ggaggagaac gtcgcccggt tggagttctc
     4681 cggtgatctc ggagcgtccg gccgaaagaa ggggcccgtg gctttccgga ccgtctggat
     4741 catcacggcg cgaaccatcc gtgccgattg cacgctgcgg ttcgaatcgg atctcgccgt
     4801 tggacacgtg ggtgtcggct cgacggcgtt gcgcggcgat ctcaacgaat tcggcctccg
     4861 cgtcgggccg gggaacaatc cgcgcgcgac gagcgccacg tacgggaagg tatcgtcggc
     4921 gggctcgacc tggatcgacg agcgtcatcc accgatgtac atggttttct tccgccggaa
     4981 tctggaaggc ttcgacatca cgacgggcac cgacctcgcc acatgggaaa gcgggcttgc
     5041 cggcaaggga ggcgctggcc ggtttcaggc gggcgtttcc aagggcggcg ccgggatcga
     5101 gttggtccgc gagccactgt cctcgatttc gcccgtgacg gtccgcaagg gcgagtacac
     5161 gttcagctac taccttggcc tgccgcgctt ggtcgagaag tccgacaggg gatggcgcca
     5221 catcgcatta ggaaaccacc cgtggccgtc ggatgagctc atccgccggt gggcggaaaa
     5281 cggagtcaat atcgcccgcc tccataacga ctatgtggcc gatgagaact tctggcacga
     5341 cggaagctgg ccgccttacg acgagaaagg gatgtccgaa ctccggcgcg tcatcgccac
     5401 ctgccgccgt cacaagatcc gcgtcgtgcc gtatttctcc attcacgagt tccaccccaa
     5461 atcgcagggc tacgccgcga atgagagcca gtggaagcgg agtgtcgatc aggccggcac
     5521 ggtcttccac aaccgcaccg gccaggggga gttcggcggc ccgatgtgtc tccagagcgg
     5581 ctggctccag cgccttaaga cgaacgtcga gcgcgcgtac cgcgagctgg gctttgacgg
     5641 catttactac gactggagcg ccgtccttcc gtgcaacaac aaggggcacg acggaaactg
     5701 gcaccttggt tcagacggca tgatggacat cctcgcatgg acgcggcggc tgatcgggtc
     5761 cgatggcacg ctgatcattc acgtctcggg ctggctgcct tcgttcccgc acgagaatta
     5821 cgcggatctg ctcgtgaacg cggaagaggc gtcgagcggt gaccggatga tgcgaaaaga
     5881 caacatcgct ctgatgacct accttgccga gggcgttccg agatcgccgt gccccgccta
     5941 cggcaaagac cgcatcgtcg agcgcaaccg caacaagctc gcgctgatgg tggcgtacgg
     6001 cgcattcccg tggtccggac aggacggccc ggtagcggag gacaccttgc ggttgtttca
     6061 caccttccag ccttaccgcc ttcaggattt tcgtctgtac aatgcggact ccggcgctgt
     6121 gcgcacttcc tgggatgacg ttcacggcgc ggtgtatgct tccccggagc aggcgctggt
     6181 ggtgctctcg aatccggaag ccgggcctcg taagaacgtc atctggcggg tttcgccgga
     6241 gcggctcggt tacgctccct ctgcggaggt gaatatccgg gacaccgcga gcgggcggac
     6301 gtcgcgcgtg ccgtggcagg ctctcgagga tggctcgctc gccaccgaac tcgacggctt
     6361 cgaatatgag ctcttcgaga tgcgtccggt acggtagcgc agcgcggtcc cacttgaccg
     6421 cacccaatat aattagggca ggaccactgg gctatgggac ttctctatca gatttcggga
     6481 ctggtgctga gcaggggcgg acgggcctcg cacgcacagg tgaaggaact tcagcgggat
     6541 ctccgcaggc tcggctacct gcgcactggg atcgacggcg actacgggaa gatcaccgag
     6601 agcgccgtca aggcgctgca attcgatctt ctgaagaaca ccggttccgg gccggacggc
     6661 tccgcccccg tgcgcatccg ggactataac aaaggccggg tcaccgcgcc gaccggcgtc
     6721 gtcgaccaac aacttgtggc gtgcatcgcc gagatgctcg gggacgaccg tttcgcgcag
     6781 gtccccgatg tgccggaccc cgccgccgag aaccgccgcg tcgccacgaa gctctcctcg
     6841 cttcggtcaa ccgaagtacc tatcccgttc ctccttggga tattcggcca ggaaagcggc
     6901 atgcggcatt ttgtggagcc gacggctgac aacgacgaca acttcgtcgt ggtggggctc
     6961 gaccgcaact ccgagagcca gacccccatc acttcgcgcg gttacggcgt gggacagtac
     7021 acgctgttcc accatccgcc cacgaaggcc gaagttcagg atttcgtgcg ggacgtctcg
     7081 cgcaacgtcg tgaaggcggt tcgggagctt cgcgagaagc tcgatcactt cgtcaacggc
     7141 aacacacccg gcacgcgcgc tgacgaccgc cagatggagt tcggacgccg gcccctgcgg
     7201 atctgcaagt acgcaccgga ggaccctcgt ttcctcacgg attgccggca gtgcatgatg
     7261 gacgccggca gcaccgacat acgcgccggc gtgacccggt tttacgaagg cgcacagcct
     7321 ttctatgagc cgacccagta tcaccccgag cgggagtaca agggagtacc gatccgccgc
     7381 aacatcgggt gcgactggcc ttacgccgtc cggcgttaca acggcggcgg cctggattcc
     7441 tatcactacc agaccaaggt cctgctgcgc gtacttcacg ggtaagatag gacgacggcg
     7501 gagggagcca acgagatgag agcgatcccg agttgggatg agtactacct gagcatctgt
     7561 aagatcgtcg ccacgaggag caaggacccg aacacgcaga ttggctccgt gattgtcggg
     7621 cccgcccacg agatccgcac gaccggctac aattcgttcc cccgagggat ccgcgacgac
     7681 gtgcccgagc ggctcgaacg tcccaccaag tacctttgga tcgaacatgc cgaacggaac
     7741 gccatctgca acgcggcgcg ttgcgggaca gccctcgacg gctgcacgct ttttgtcgag
     7801 atcatgccct gcatggactg tgcgcgcgcc atcgtacagg ccggcatccg gcaggttgtg
     7861 atctccggcg aacgcatggc gcagtactca agcgattact acaaccagca cttcggaatg
     7921 gtcgaggtgc tctttgctga agccggtgtc gctgtccgcc gggttccgta gcccgccgcg
     7981 ccccccttgc gccccgagcg ggcgcgcagt acagtgcctc atagaccggg tgcaacgccc
     8041 ggaggcaagg aggctgctca tgcctgtcac cgtgaaaccc gtcaccctct ggcggaaaga
     8101 aatcgaaaac aaaccaggga ccctcgctgt ggcgctcgaa ccgcttgcca tggcaggcgc
     8161 tgaccttcag gtcattatgg gttaccgttt ccccggcaga tccgacgccg ccgccctcga
     8221 actgttccca gtgaaaggga agaaggcgac tgcggcagca caggccggag gcctcagcgc
     8281 atccaccatc cctacgctgt tggtacaggg cgacaacaag gccggcctcg gttataccat
     8341 cgcgacgtca atcgccggcg ccggcatcaa catcggcttc gtgatcgccc aggttgtcgg
     8401 gcgcaagtac tcggcggtga tcggcttcga tacgtctgat gatgcgaaga tggccgccat
     8461 tctcatcaag aaagctgcgg cgagcaagaa gaagtagtgc cgggcgttca ctcgcgcagg
     8521 ggtggtacaa tgccggtctg cctgcctgtc gaaggcggtt ctgacgaatg cgcttcccgg
     8581 gaagtctttt tctccacaac ctgattgagc gccgagccct gctgttccaa ctcgttcgcc
     8641 gcgattttga gcagcgcttt gtcggctcgg ccgccggctg gctgtggggc gtcatccacc
     8701 cgctcgtgct gctgctgagc tggactttcg tctttgactg gtgcctcaac gtccgccttg
     8761 gagcggacgc agcgacccag aactacccgc tcttcctgtt ctccggtttt ctgccgtggc
     8821 tgctgttcca ggataccgtc cagcgctcct ccggcagcct gctcgaacac gctaacctca
     8881 tcacgaaaac ggtcttcccg tccgaggtcg tgccgctgtc gatcttcctc tcgtctctcg
     8941 tgcaccacct gatcggacta gcgctcgttg tggcggcagt ggccctcttc ctgaaccact
     9001 ttggagtgat gatcctgttc ctgccgttgt atatggcact gctcggtctt tttgctgtcg
     9061 gaatcggatg gattgtcgcg gctctccagg tctacctgcg cgacacggcg cacgtgctgt
     9121 ctgtcgtgtt gacattatgg ttctggctga caccgatctt catcacggaa tcacggtacc
     9181 cgccgccggt gcgtttcctc ctcgcgatca acccgctcgc gcatattgtt cgagcttacc
     9241 gggagcgcat tctgacgtac cgcctgccgg atgtggaaga attagcagtg gtggccgcgt
     9301 tttccgcgat caccttcttc gtcggcggcc tgttcttccg gcacctgaag cggggtttcg
     9361 ccgacgtctt gtaattcagg agtctcgccc gtgtctttat cgcgacgttc gtttctgctc
     9421 agttccgccg cgcccgcgct ggcccagaga aaaccgcagc ccgcccgccc gaacatcgtc
     9481 ctgatcgttg ccgacgatct cgctgcctgg atgctcggct gttacggcaa caaagagata
     9541 cgcacgccga acatcgatct tctggcgcgc agcggcacgc gttttctgaa caacttcgtt
     9601 tgcactccga tctgttcggc cagccgcgcc acgctgttca cggggcgcac gccgcgacag
     9661 cacggcattc acgattttct gacggagaaa ccgatcgaga agcccccgca gggccaggcg
     9721 gccccgcctc cctcattccg cgacgaggtc atgatttccg acgttctcgc ggggaatggc
     9781 tatcagtgcg gctacgccgg caagtggcac atgggcgacg accagaagcc gcagcacggc
     9841 atcaagcact ggtacaccat gctcggaggg tccagcccat atcagaaccc gcggatgagc
     9901 ttaaacggag aaatcgttca ggagcagggc tatctggctg acctgatcac gcgcaacgct
     9961 accggcttca tcgagcagca gtcgcccgcg aatccgttct tactggtcgc cagctacttc
    10021 aacccgcacg ttccgtacga cgggcacccg ccgaagtact acgacattta cgcgaagact
    10081 acatttgaga ccgtgggttg ggagccaccg gcgccaaatg ccctccgcga aaaggaatat
    10141 ctcaccgaca tcatcggtaa cctgaggagg tgcgcggccg cggtgaccgc tctcgacgat
    10201 caggttgcca cgctcatctc cgctctcgac cgcaagggac tccgcgacaa cacgctcgtc
    10261 gttttcactg gagacaacgg cttcctcctc gggcgtcacg gtctctggag caagggacac
    10321 gcctccgacc cgatcaacat gtacgaggaa gtcgtccaga cgccgatgat ctggaactgg
    10381 ccagggaaga ttccggtgga ggccgttcgc cccgagctcg tcagtttcta tgacgtaatg
    10441 ccgtcggtgt gcgacgtcgc cggcgtggcc actccggccg gccgcaatct ctgtggacgc
    10501 agctacgccc cgcttgccat gaaccgtccg ctgccacgga aacaaccatg gcaaaacctc
    10561 gtgttcggcc atttccgaaa cacagagatg gcacgggaca aacgattcaa gctggtcatc
    10621 cgcgatggcg gcgagggacc gggcgagttg tacgacctag tggctgatcc gcgcgagaag
    10681 gtcaaccgct acaacgcgca agagttcgtt acggttcgcc atcgcctgag cgccgaactt
    10741 gaggtctggc gcaagaagta cgcctgacgc gagaatcgga cgctcgtcag tctgtccgcg
    10801 agtcgcgata gaattccccg aggaacatgc cgaaatccgc gacggttctt ctgctttgcc
    10861 tcgccggatg cggcacggcg ctgctgtgcg ccgccgcact gtcgttcgag cgcgtcgaga
    10921 ttgacgccgc tccgcctcaa aacccgtgga tcaaggttgc gggcgacctc gatggcgacg
    10981 ggaaagccga tgttgtcatc ggcggcagca agggtccgct ggtgtggtac cgaaacccgt
    11041 cctggaccaa gtctgtcatc gcggccgggg gctacgactc agtcgacgcg gaagcggcgg
    11101 acatcgatgg cgatggcgac ctcgatctcg tcgtcggcgg cctcctctgg tacgaaaacc
    11161 ctcggccggc gggcgatcct ggaaaaagtc cctggcgcgc gcatcgcatc gcctcgcacc
    11221 acacgcacga cgtcgaggtt gccgatctcg atcgcgacgg aaagctagac atcgttgtcc
    11281 gtggccaatc cggcttcggc gccaacgagg gtcaccgcat ctttgttttc cgccagaacg
    11341 cgcgggactc ttgggcctcc cgcgagatcg aatgcccgcc cggcgagggg ctcaaactcg
    11401 cggatgtgga cgccgacggc gacgccgaca tcgtcgtcgc cgggcgctgg ttcgagaaca
    11461 ccggcgccgc gttggccggt ggctggcacg aacgcatcta caccagcgcc tggacttacg
    11521 aggacagcgc cgtcgcaacc ggcgacctca acggcgacgg ccgcctcgac atcctgttga
    11581 ctccggcgga atacaagggt ggttcgtatc gtgtcgcctg gtatgaggcg cctgcgaatc
    11641 cgatgtcggg ctcatgggcc gaacacgtgg tcgctgcgcc cgtggaaacc gttatccacg
    11701 ccgccgccat tgccgacatg gatggcgaca aagccccgga cctcgtcaca gcggccatgc
    11761 accagggcgc cgcgcctcag gaggtcacgg tgtttctcaa tcggggaagc ggccttcgct
    11821 gggagaagtc cgtggtttcc acccgcggct cgcacaatat cctgctggtg gacctcgacg
    11881 gcgacggccg catcgacatc ctgggcgcca accacgccgg accatacagt ccggttgagt
    11941 ggtggcggaa tacctcgaga ttgacgcgct gacgacccct gccgccaccc tcgcacaaac
    12001 gtctgagcaa gtactctgaa gcgaccgcat actctaaaca gaatgcattg acaggagggg
    12061 atgattgtta acgcgttaag agtgtgtggg gcgttcgact accgatggtc cgcagatagt
    12121 ctctgattgc caacgctgcg cgcacatagt gctcgcgagc ctcatcgtaa gcctcttgag
    12181 tgagcatcgc ttgagactct cctccccgag cgcgcttgag gtcgatgtac tcaccgagcc
    12241 gatccgggtc gaacttacag tcattcacaa ccgtactcaa ccttttttcg tcagccgggt
    12301 ggcgagtgtg ccaagtctga acctgagaaa cgaatcgaaa gcttcgtaca tgaaccgtat
    12361 tctacagcca gtattagact gactgctcct tacggccgct tacaataact cccgcaggct
    12421 ccagatctga tccgcgaccc cgcctccatc gccgacgcgg cccgaaacgt ccggagaacc
    12481 cgctggaagt tgcaggacgt gcatattcgc tcgaaagcgg gcccccgagg atctcggtct
    12541 gaagatcgca atatgcgtgc atttcgaata agaatgaagt atgccgtccc ttcaactacg
    12601 tgatgtgcca cccgagttgt accaccgaat cgccgacctg gctcgccgtg aacatcggag
    12661 cctcacgcag caagccgtca tcctgctcgc ccgcgcattg gatgtttccg aggatcccgt
    12721 caggcgtagg cggcgcctgc tcgacgctgt ttctgggcgg ccactggtcg ccagcctcga
    12781 tggggtgcct ccgccggaag cgctggtgtg ggaggaccgc aaaggatgac aacggtttcg
    12841 tgacaagcgc caaaagcaag ttcgtcaaga atctctggca cagtcttcct cacctgccct
    12901 gttccctcaa gcggcggtat ttcctgagga gttcgtagag gacgatgccg ccggccgttg
    12961 ccacgttcag cgagtgcttg cgcccgagca tcgggatgcg cacatgcgtg tcgcacaacg
    13021 cggataattc cggccggata ccctctgttt cgtgcccgaa caccacgcac acgggaaagc
    13081 gcggcgccca atcgaagaga tccaccgcgt gcatgctggt ttcgatcgcg gcgatttcgt
    13141 agccgtccgt ccggagccgt tccgcaagcg agaccggttc ccaggaatgc tcccaaggca
    13201 ccgtttcctc cgcgcccaga gccgttttgc tgagggccct cttcggcggg tgcgcggtga
    13261 tgcccgagag gtaaagcttc tccaccgccg ccgcgtccgc cgtccggaaa aatgcgccca
    13321 cgttgtacat gctgcggatg ttgtcgagca gcaccgccac gggcatccgg acgatgccgt
    13381 catagggctc cgcggccccg gtgacctgga aaggcaggcg ttccacgaac agctatctta
    13441 cccgcccccg ctacccaccg gccgctatgc cacaatttcc cgtatggtct ctctggacgg
    13501 cgagagtctt tcccttgagc aagtctccgc ggtggcaatg aagagtgagc ctgtctcact
    13561 cgcggcctcg tcgctcgaga agatgcgtcg ctcgcgcgct gtcgtcgaac acctcgccga
    13621 aggcgaggag cctgtctatg gggtcaacac gggcgtcggc cgtctcgcgg acgtgcgcat
    13681 cgcgaaggaa gacctcgagc agttgcagcg caatatcgtt cgctcccacg cctgcggcgt
    13741 cggcgatccg ctcagtcgcg aagaagtgcg ggcaatgatg ctcatccggg cgaacgtgct
    13801 ggcgaaaggt ttctctggca tccgtcccgt tgtggccgag cggctctgcg accttctgaa
    13861 ccatgacatc atcccggtgg tgccgtcgcg agggagcgtc ggcgccagcg gcgatctcgc
    13921 gccccttgcc cacatggcgc tcgtcctcat cggcgaaggc gaggccgaat tcgaaggcgc
    13981 gcggtggcag ggcggggaag cgctgcggcg ggcgggctcc cctccgctca cgctcgcttc
    14041 caaggaagga gtttccctgc tcaacgggac ccaggcgatg ctcgctgttg gctgccttca
    14101 gcttcacgcc gcggaaatcc tggccgacgc cgccgacctc gcttgcgcca tgacgatcga
    14161 tgcgcttcga ggcactcccc gagctttcga cccgcgcctt cagcaggtac gcccgcaccc
    14221 gggccagctt cgaagcgccg ccaacctggc taggctcctc gatgggagcg agatccgggc
    14281 ctctcacatt tcatgccgcc gcgtgcagga tgcctactcg cttcgctgcg cgccgcaggt
    14341 gcacggcgca gcccgcgacg cgctctcgga ggcgcgccgg atcttcgcca tcgaactcaa
    14401 ctcggccacc gacaacccgc tcgttttcga ggacgagatc ctctccgggg gtaattttca
    14461 cggacagccg ctggcgctcg tgcttgacta catggccatc gccatgacgg ccctcgccgg
    14521 gatttctgag cgccgcatcg accgcttggt aaatcccgag cgaaacgaag gcctgccgcc
    14581 gtttctcgcc gcccacgccg ggctcgagtc gggcttcatg atggctcagg tgaccgccgc
    14641 cgcgctggct gctgaaaacc gcacactcgc agccccggcg tctccgggtt ccatcaccac
    14701 cagcggcgac aaggaggatt tcgtcagcat gggcatgacc gccgcgctga agctgaaaca
    14761 ggtagtcacg aatacccgga ccgtgctcgc catcgagatg atgaccggcg cccgcgcgct
    14821 cgactgcctt gccccgctac gttccagccc gatgatcgaa aagctgcgga tgtgcctccg
    14881 tgagatcacc ccgccttatg agactgatcg cccgctgtcc gccgacattg agcgcatcgc
    14941 cctctgcatc tcccatggcg gcccgtcgga tctcctggcc tgatccacgc gcggaaccgg
    15001 tcccttctac gtgctgtaca cctggaaccg cgtcaccacc tcgtagccca tctgcttgta
    15061 gagtgacagt ccttcggcgg aggattgcag gatcgtccgt tcgaagccgg gctcttccct
    15121 cacctttttc agtgcgtgac gcaccaccgc ctcggcgtat cccttccgcc cttcttctgg
    15181 cagcgtcgca acgcagtaca cgcccaccgc ccccgacacc gccagggtcg ctgccgccgt
    15241 caccggccgc ccgttcacgt accccaccca gccggtgaat tggtcgttcc ataggggttc
    15301 caggttgtac aactcacggc accattcgaa cggcatccga aacgcagcgg cgttgacgaa
    15361 gcagaaatcc agccgctcgg gctctttctc gacccggcgg aaatccaaat tcgggagcct
    15421 gcgcgatggc ggcagcagcc ggtccgcggc catcccgggg ttctcgtagg caaggctgag
    15481 gccgtgagct tgaaagaccg cccgcgactt ctttcggatg gccgcgggaa gcttgtgctc
    15541 cgccgcccag aatgcccatc gcagcccgcg ggcggcatag tgaaccgacg ccgtcaggat
    15601 tctgcgctcc agcagcccgg cgccttccgc caccggctcg gagagaaacg ccccgttgaa
    15661 ggtgtgaatg ctcgcgccgc aggatgcgat cgatacgccc ggcatctccc tgacctcgcc
    15721 cgacgaacgc gcgcgtgcaa agtgccggaa gaactccacc agattctcct cgacgcttcc
    15781 cgcccgatcc atggcggcgc tcacatcagt gtcagcggat ccacgtcgat caccaaagcc
    15841 gtcgcgttcc atttcgactc cagcgcgaag tcccggactg tgcgcagggt ttcgttgagg
    15901 accttccggt tcgccgcttt gatcagaagc tggtaccggt attcgtttcg cagccgggcc
    15961 accggcgcct cagccgggcc gagaatgcgc agaccttccg gcggcgggtc caacagacgc
    16021 cccagttccc cgctcatcct catcgcttct tcctgcttct cgctccgcac aagcaggttc
    16081 gccagtgcgc tgaacggagg atagcgcatc gaccggcgga actggatctc cttctcatag
    16141 aacagccggt aatcctgcgc cgccgcgaaa cggacggcgt agtgatccgg gttgatcgtc
    16201 tgcaccagca cgatgcccgg cgtgtcgccg cgcccggcgc gcccggccac ttgcgtcagt
    16261 aattggaacg tccgctccgc cgcccgaaag tccggcatcc caaggccgat gtccgcgttc
    16321 accaccccga ccagcgtgac gttcgggatg tcgtggccct tggcgatcat ttgcgttccc
    16381 accagaatgt caaagttgcc gtcgcggaac ccttgcagga tcgtttcgaa ctgacgcttg
    16441 ccactgaccg tatcccggtc catgcgggcg atgcgcgccc gcggaaacgc gcgatgcagt
    16501 tcttcctcca cccgctccga tcccacgccc aggaagtaga tgtgctcgct ttggcattta
    16561 ggacaggcat tcggcacttt gatcgcgtag ttacagtagt ggcacagcat ccgccgctcc
    16621 cgccggtgga acgtcatcgt caccgagcaa ttgacgcatt ccagccgttc gccgcacttc
    16681 cggcaggcca ggaaactcga gaagccgcgg cggttcagca gcagcatcgt ctgctcgccg
    16741 ttttcaagcc gctgctgaac ctcttcgacg aggcgccgcg aaaatgtgga ctgcgtgcgc
    16801 gtctccagaa actccaggcg catgtcgatc agttccaccc gcggcagggg ccgttgctcg
    16861 atccgttccg gcaattcgag cagcgagtat ttgccgcgct cggtgttgta tcggctctcc
    16921 aagctcggcg tggccgagcc gagtatcacg cacgctccgg cgttcttccc tcgcaccacc
    16981 gccacgtccc gcccgttgta gcgcggcgcc tcttcctgct tgtagctctg gtcgtgctct
    17041 tcatccacca cgatcagtcc tagattacgg accggagcga atacaccgga gcgcgttccc
    17101 accactacag gcgccgcgcc ggagcggatt cgccgccact gctcggcccg ctccgcatcc
    17161 gtgaacgccg aatgcagaat cgccacccgg tcgccgaacc ggtggaagaa ctggccggcc
    17221 attgcgggag tgagcgcgat ttccggcacc agcagcagcg cgccgcggcc cagcgcgagc
    17281 gctgtgtcga tcgccctcag atagacctcg gtcttgcccg aacccgtgac accttgcagg
    17341 agatacgtcc ggaactcgcg cgcctcgatg gatcgccgca ccgcgttgaa ggccgcctcc
    17401 tgatgcggat tgagcacatg cggcggccgt ccgggtagcg acgccagggc gaggggctct
    17461 ggcgtgagtg atgcgaatcc ctttcgcgcc agcgaccggg cggcgccgct cgcatttttc
    17521 actgcctcct cgacttcgcg gaggttgtgc gaacccggat gcagttcgag atacgccagc
    17581 agctcgcgct cggctttcgg gacctttgtc ccatcgggat gacgaagcag ctccaggcgc
    17641 aactttggcg acggcgcgcg caacgggtcg cgctcgaagc gcacttcttc ggcgtacacc
    17701 aggcctttgc gttcgagcga gcgaagcatc ttctccgcca gaggaacttt cttcccgatc
    17761 gaagctgccg tcagcggacg cgcctcgagc acgcgcagga cctctgtggc agggtcgtcg
    17821 ggagaatcgt cgagcagaag ttgccgtgtt gcatcccggc cggcgtccgt cagcgagtac
    17881 accttgctgc tgcggacctc ggcggcgagc ggagtcatga tgcgcagcac ctcaccgagc
    17941 ggcgcgcaat aatacgctcc gatccaacgg ccgagcgcca gcagctcgtc gtccagcacc
    18001 ggttcggagt cgagcagccg cagcgcccgg cggacctcca tcgctggcgg gccgtcgtgg
    18061 cagcgcaata ccacccccgt cagcttccgc gagccgaacg gtacgatcag ccggcagccc
    18121 ggtttggtgc gatggcggag agtctccgga agttcgtagg tgaacgcctg atcgagcggc
    18181 accggcacgc tcacgtcgca atatcggtaa tctgcctcgg gcatggcctt actcatacta
    18241 tcgtttctgg gtttcacaga actccgtgat caccgcgtgc cttgcggccc gctatgagca
    18301 tcgctatatt cggggtgttg ctgtgtgtct ggaggaactt gcgaaacatg actgatgcgg
    18361 ccggagaccg gctcgactca tggaaagaga tcgccgccta cctcaagagg ggcactcgga
    18421 ccgtgcagag atgggaagcc gaggaaggac tgcccgtccg ccggctgcag cacgataaac
    18481 tcggttcggt ttacgcttac aagaaggagt tggacgcctg gtgggcgagc cgcagacccg
    18541 ccgaagaaag ggagtccttg gtgggggccg agagcggcgc atcgatcgcc gtgctcccct
    18601 tcacggacat gagccaggag aaggaccagg gatacttctg cgagggtatg gcggaagaga
    18661 ttatcaacgc cctcggacgt cctaacgggc tgcgggtggc ttcacggatc tcttcgtttc
    18721 agtttcgatc ctcgagcgcc ggcacgcgcg agatcggccg taagctgcgg gtcggcacga
    18781 ttctcgaagg cggcgtgaga aaatcgggca accggctgag gatcacagtt cagcttacca
    18841 atgccgagga cggttatcag ctctggtccg cacgctacga ccgggaaatg agcgacgtgt
    18901 tcgcgatcca ggacgagatt gcggagaacg tcgtccaagc cttgaaagtg accctcagtc
    18961 ccggagaaag ggccgctcgc gagaaggcgc ccaccagcga cgtccaggcg tacgacctgt
    19021 atctgcgcgg gcggcactat ttctatcact acggaacgcg ggacatcgag ttcgcgcgaa
    19081 acctgttctc ccgcgcgatc gaactggacc cgcactacgt gctcgcctgg gcgggcctgg
    19141 ccgactgctg gtcgtacctg ttcctgtacg cccagcgcag cgacgagaac cggcggcagg
    19201 cggaggaagc gagccgcagg gcggtggagc tggaccctga gtccgcgcag gcgcaagcgt
    19261 cgagggccgt ggctctgtca ctgagtgagc gaaacgagga agcagaagcg gcattcgaga
    19321 aggccatccg gatggatccg gccctgttcg aagcccacta cttctatgcg cgccactgtt
    19381 tcgttcacgg ccgggtggag gaagcagtcg cactgtacga gcgggccatg caggtgcgtc
    19441 ctgaggacta ccaggtcccg ctgctgatgg cgcaaatcta cgacgacctc ggccgctggg
    19501 atgtcgccgc cgagacccgc tgccgcggcg tggagaaggc cgaaaagcac ctggagtgga
    19561 acccggacga cgcgcgggcg ctctacatgg cggcgaatgg gctcgttgct ctgggtgagc
    19621 gggaacgagg cctggaactg gccgagcgcg ctctggcgat gcggcccgac gagccgatga
    19681 cgttgtacaa cgtcggctgc atctactcac tgctcgggcg tgtcgagccc gccgtccatt
    19741 gtctggaaaa ggcggtcgga aacgggcttg ggcagaaggg ctggttcgac cacgacagca
    19801 accttgaccc gatccgcaca cacccgcgtt tcgcccgtct gatgaagaaa ctggcgtaag
    19861 cgtggccgct cctccctttt ggtcgcaggt tcggaaagtt ggtcgcgagt ctagaccgtg
    19921 ccccgcaccg cccatgcacg tgcgggaaga gtgaaaggcc ggtcatccgc agataacgac
    19981 aatcgtcgct tcaccgcacc gcgcaacgcc tccaagcgat cgcggccgag cgtgcgagcg
    20041 tacgcgccgg caggaccctg cccaaggagg aacgcatccc agtaatccgc gaaggactcg
    20101 aaccgcatcg tgatgtccaa tggctgctcg tggacgctct taaggccgcc ctgtttccat
    20161 agctccgaga gttcactagc gcggcacaac ggcatgtgct tttcgtcaaa cctctcggcc
    20221 cgcgcgtcta tgctgacggc ggcatcccag aagaaccgga gcatcctcat ccccgcaccg
    20281 tagtcccaga cagcggctga gattcggccc ccggctgtgg ttacacgacg cacctcccgg
    20341 agcgccttct cccgatcggg aatgaaattg aacacaagca aggaaagagt ggccgcaaag
    20401 gctgcatcgg ggaactgcag ctgctgagcg ttgccgacct gaaatcttac gcggccaggg
    20461 aatggattcc tgctgtttgc gtaggcaacg tacgcctcgg acggatcgat gcccaagacg
    20521 tgcgaccgct ccttgcgttt cgcgattgcg aaggccaacg atcccgtgcc tgatccaacg
    20581 tcaagtgccc gtccctcgtc cggaagacca gaaaaatcca ccagtaaggg cgctacgagg
    20641 tgactccacc gccccatgaa tcgttcgtag gcttcggcat cgccgaacat atttggccct
    20701 tcatccccgc cggggatcat cgggactgca aggatgcgga gagcgcccct ccgcgaaagc
    20761 atacgcccag tgtagttcac ccgctgccag gatgagcgag tcagccctgg cactcacccg
    20821 ctaaagatcg cccggttccc gccgattaac gtaaagggcg ggatgccgcg cgagtgaacc
    20881 gtcaactgtc acagcaggag atcgacgccg tcttcgagaa cctcggcaag ggcgagggcg
    20941 gccggacagg cgtgctgaag acggcgccgt tcgactttcg ccggcctgat cgcattccaa
    21001 aatcgcaact acgcgccctg cggctactgc acgaaaattt cgtccgaagc ctggcttcga
    21061 gcctgtccgc ctatctgcgc acatacctga cggcgaatct tgtcagcgtg gagcaattgt
    21121 cttacgcgga gttcgtcgag acactttcct ccccgacctg tctggtaagt ttagggctgc
    21181 ggccctacga cggacatgcg gtgctggaac tcggccctag cctgaccttt cagatactgg
    21241 aggtcattct cgggggaaag ggcaaggggc cgccggcgca gcggcgagag atcaccgaga
    21301 tcgaacgaag cctgctcgac ggtctgcttc acatcatcct gagtgagttg aagcaggcat
    21361 gggagtcggt cacggccatc gagtttacgg tggaatcgct ggagacggag ccccagttcc
    21421 tgcaagtgcc ggatccgggc gaggcgtttg tggcggtcac gatagagttg aaggttggcg
    21481 aggtggcagg cctgatgaac ctggcattgc cgtcgctgat catcaagatg atgcgggcga
    21541 agttcgacca tcatgggtcg ctgcggaaga tgcagtcaag cggggaagag caagcgcgga
    21601 tgctgcggct gatgaagccg gcgaagctgt ctttggacgc acggctgcgt ggtccttcga
    21661 tgctggtgcg ggatctgttg agtctggcgg agggtgatgt cgtaccgctt gactatgcgg
    21721 ttcagcgtcc actggagttg ctggtgaacg gccggctgcg ctttcggggt cagctcgtaa
    21781 gcgccgggaa caagaaggcg tttgtaatcg gcgggcgcga agagcagcag cgttgagatt
    21841 cgggtgtctc ccttgggctg gcggccggcc ggctgaggtg tttgagaagc ctgacgcagg
    21901 tgtcgggaca taccacgctc gcatcgccgg tggcccgcat gcgttccagg atgccttcca
    21961 gcagatccca gcgttcgtct tcgtcgccgg agaccgaggt acgggcgacg cgggcgtcgc
    22021 ggacgcggtc taggtggggt tccaaccgga tcggtttgtc gtgctggatg gcgctgagga
    22081 ttctgccggc ttccagagcg gggctggtcg agatggcacg ctccacgttt ctctccctcg
    22141 ctcttcctta tcggtacggc gcgggtgttt tcgagaaatt ctcgactcga tagaagccgg
    22201 ccgcggcccg ccgatatgat tccttggggg tgacatatga tccggctgac gcggctcaac
    22261 catctgcccc ttgtgctcaa ctcggatctc atcgaacaca ttgaggtgac gccggatacc
    22321 gttatctcac tgaccacggg acagaaaatc atggttctgg agacggcgga ggaggtggtc
    22381 gggcgggtca tcgagttccg ccgagcgatg ttccacggtc tgctcgaatg cccagggcgg
    22441 ctgacgccgg cgggggagcc cgcgggagtg gagggattct gagtaccaat ggccggcgtc
    22501 gcagagggaa agtccaagcg cgggcgaacg aagccggacc tggcttcctg gggaggtctg
    22561 ttgctcgctt ccaccgggat catcgggggg ctgctgctcg aaggtggcac gattcaggac
    22621 atctcgcagg tgacggcggg aatgatcgtt ctgggcggca cgctcggcgc ggtaatggtg
    22681 accacgccgc tttccgtgtt gctgagtgcg gcgcggaaac tgaagctcgt cttcttcgaa
    22741 caggcgcaga cgccggacgc cgtgatcgag gagatcatca cctacgccac caaggcccgc
    22801 aagaacggga tcgtttcgct cgagcaggaa gccgaagcgg tccgcgaccc ctttctcagg
    22861 aaggcgctca acctggccgt ggacgggatg gacctcaaag agcttcgcaa gatgatggag
    22921 ctcgagatcg ggctggaaga gcaacgtgcc gaggctgagg ccaaggtcta tgaagcggca
    22981 ggggggtacg ctccgacaat cggcattatc ggggcggtac tcggtcttat ccaggtaatg
    23041 aagcatctgg agaacatcaa cgaggtgggg aagggaattg cggtggcctt cgtggcgacc
    23101 gtttatggag taggctccgc caacatcttt ttcatccctg cggggcacaa gatccgggca
    23161 cggatgcatg gcgtcctcca gactaaggag ctgatgatcg agggcgtgac cggcatcgtc
    23221 gagggactga acccgaagct gattaggatc aggctcgaag cctacacgcg gcgcgacgca
    23281 aagacgaagc ccgccaaggg gagcaaagaa gcaaagccgg cgggcgaacc agccgccatc
    23341 agttcgtgat cgaagagact gcacgtggcg aggaagaagc aagcccagca cgacaaccac
    23401 gaccggtggc tcgtttcgta cgcggatttc atcacccttc tgttcgcgtt cttcgtaatg
    23461 atgttcgcct cgtcgcagac cgataggggt aaggcgcagc aggtctcgga gtccgttcgg
    23521 aaggcgctcg aagagactca cgcggtgtca cggatcgtgg caattctcgg gggctccgct
    23581 aaagatgcgg ggaagggcga ctcgatggcc gccccgaagg acccgggggc tccgaacgag
    23641 acagtcgttg accttctacc gtcgctcgag ttcttgtcac gggagttgtc tgcggagatc
    23701 cgcgcgggca agatgcagat caacctggag ccgcgcggac ttgtagtcag cctgccgcag
    23761 gcgaccttct tcccgtcggg ccaggacacc atcgacccgg ccacgtatgc agtgatcgaa
    23821 aaagtgtcgg cggcgatccg caagattccg aacccggttc gcctggaggg acacaccgac
    23881 tcagtgccca tccacaacgt tcgcttccgg agtaactggg agctgtcggc ggcgcgcagc
    23941 attgcgatgc tcgaactgct ggcggtgcgc tacgaaatcc cgcgggaacg gctggcggtg
    24001 gccggttatg cagacacagt gcctgccgag ccgaacgatt ccgaagaagg ccgggcgcgg
    24061 aaccgcaggg tggacatcgt cgtgcttagc cgaatcggcc taatgcgcga gccggagccg
    24121 ggggcccgga cggagccgcc gccatccgcg aagggaccaa agggcgcctg attcgctccg
    24181 gggcttggtc cgagggcggc agtccgattg caagggatct accgacggtc cggctgtatc
    24241 ggactttatc tatgccggtt tctcacacca ggtatttcgg gacgattgac tacagggacg
    24301 agtcggtggt ccggtttccc cacggtctgc cgggcttcgc ggaggaaacc cgatttctgc
    24361 tgatcgacca gcccgtgaac gagccgctgg tgtttctcca gagcctctcc cggcccgacc
    24421 tgtgctttct ggcgctgccg gtgcttgcgg tctgtccggg ttaccagctt tcgattccgg
    24481 ctgaggatct agccactctc gggctccccg aacagccgca gccggagatt ggggcggagt
    24541 tgtcctgcct ggcgatcgtc tcgctcgccg aagaccggcc gcccgtagcc aacctgctgg
    24601 caccggtgct gatccattgg aagaaccgga gggcggtgca ggcgatccag gctgaattcg
    24661 ggtactcgta tgagcatccg ctattcgcgg aggctgcgcc gtgctcgtga tccggaggcg
    24721 cgccggagag tcgatcctga ttggcgacga catcaggatc gagatcatcg aaacgggcgc
    24781 cgcgcgggtg aagctcggaa ttaccgcccc gagggacgtc ccggtgctcc gcaacgagat
    24841 ccggttggcg ggagaggcga accaggcggc ggcgcgggag gtgaccgcag cggcattgag
    24901 ccgcctggtc gacaggctgc ggagaacgtg aaccgcatac gacaaaatgc cggcgaatga
    24961 cacgatttcg tcgcggcgac ccgcttcagg cggcagcccg caattgccgc acgaagcgct
    25021 cgaactcctg gcggagcggc cgatatgaac actggcggcc gagagattca ggccgctccc
    25081 ggcatgaagc cgggaacctt caaacaaaca aggatgtgat tcaatggccg tctcaattca
    25141 aaccaacgtc aattccatcg ttgcccagga gaacctccgg gtgaacggtg agttccagag
    25201 ccggacgatc cagcggttga cctccggctt ccgcatcaat tcttcgggtg acgacgccgc
    25261 gggcctggcg gtagccaaca agttccgcag cgatatcgcc gaactgacac agggcgtgcg
    25321 caacgccaac gacgccatca gcgtgctgca actggtggat ggcgggctga acaacgtctc
    25381 caagctgctg gaccggctga agacgttggc cagccagtcg gcgtcgggga cgttcaccgg
    25441 gagccggacc acgttgaaca acgagtacca ggcgctgctg acggaaatcg accggcaggc
    25501 ctcgaacatc ggcttggtgg gcggcggggc gaacaacaaa gttctcgaca tctacatcgg
    25561 tggcggcagt acgcaggcca acgctcaggt gagcatcgac ctcagcggca ctgccaacca
    25621 gacggactcc gccggcctcg gcctcgccaa tactgcaatt tcgggcggcg ggacggcgct
    25681 gacgggcaac tcggtccgtc tcgacaactc ggcgctttcg ttcctcaacg gcagcgacac
    25741 gcaggcgttt actttccgga tctataacgg ctcgggtacg gagaacgtgg tcgtgacggt
    25801 ggccggctcc gcctctggca tcagcgggac cgaagtgctg aaccagttga acagcgccct
    25861 aagcacgtac ggcatctcgg catccacggt aggatcgggt gccgatgtcg gcgagttgca
    25921 gttcggcggc tcgcgggctt tctcggtttc ggtggctgct ccggccggcg cgggccaggc
    25981 agcggtgacg gcaggcagca cggccgtcaa cacgggcaac taccattacg atgtggcagc
    26041 agccgatttc gttgcggttt ctgccggctc ggaggttgtg acattcgcca acgccaacgg
    26101 caccgcgaac gtgaccttct ccaccacttc cggcaacacg gtggcgaact cgatcgcggc
    26161 cattaacgcg caaacggcgt cgctcggtat ctatgcggtc gccgatgccg cgggcaccgg
    26221 gttctcgatc caaagctcca gcagcttcac cctggtgaaa acccaggctg cgggcacggg
    26281 cgtggtgatc acgggtgcgg tgggcgccaa gacggtgacg gcgcctgcgg ccggcgcaag
    26341 cgccaccggc aatgcgctgt cggcgatcac gtccctgacg tcggcggtaa cgaccctcgg
    26401 cctggtacag ggccgggtgg gaaccggcca gaacaagatg ttctacgcga tccaactggc
    26461 ccagtcgcag atttccagct tctccgctgc cgagtcgcgc attcgcgacg cggacatcgc
    26521 ggcggaggcg gcgaacctga ccaaggctca ggtgctggag caggcgtcgc tggcggcgat
    26581 ggcgcaagcc aactcctcgc cgcaggcggt gctggcgctg ctgagggcgt agaggctcgc
    26641 agcttgcagc acgtagcaaa cggctcgcgg cccgtggaga ctgattcaca ggccgcgagt
    26701 gtctgaggcc aaagatgaat tcgacttctg ccatcttcaa cggcaccagc cggtactcgg
    26761 cggactttca gcagatcatc gaccgcgcgg tggccatcgc ctccctgccg atgatgcaga
    26821 tggagagcgc gaagacggcg ctcgacgatc aggccaaggc cctcgagagc ctcgacacac
    26881 gtttcgcggc ggtcgatagc gctatccgaa gtctggagtc ggccgccgcg aacaactcgt
    26941 acgtgctctc gaactcggac ggcggcgtgg tggcggccag cgtgtcgagt ggggcgctga
    27001 ctggtgttta cacggtggag gtggtcgagg ccggttcgta cacgagctct atgagcaagg
    27061 acgggctgac ggcggtcgcc gacccgtcgg cggagagtat cagcctggct tcgacgttca
    27121 cgctgacggt ggccggggag acgtatgaga tcacaccctc tggcgacact ctcacggccc
    27181 tggcggcggc catcaatgcg gagacagagg ccagtgtgga agcgacgatc gtcaacatag
    27241 gaggcccgtc ggcacccgat taccggctgt cgctaaggag cgccaggctc ggcgcggttt
    27301 ccatacagct caacgacggg tcggccgatc tggtggacac gctggcgtcc ggaacgccgg
    27361 cgagttaccg cgtgaacggg caaccttcca cggccgtctc gaccgactcg cgctcggtga
    27421 cggtggcgcc aggacttacc gtcacactgc tgaaggcggg cacggccaac ctgacggttt
    27481 cgcgcaacac ggccggtatc agcaacgcgc tggcctcgtt cgccggcgcc tacaacgccg
    27541 ccgtcgatga agtggacctt caccgcggca aggatgccgg agcgctgaag gggaacagtg
    27601 tcctgttcac gctgtcccag accttgcggg agattgcggg ctactcaacg ggcacggacg
    27661 gtattgcttc gctcacctcg ctcgggctcg ctttcgacga ccagggcaag ctctcgttcg
    27721 acgcgaccgc attctcgaac gccgtcgccg ggaatctcag cggcctgctg tcgttcctcg
    27781 gcagtgcctc agagagcgga ttcctgcaat cggccaccag tgcgatggac gcggtcgtgg
    27841 gtacaaccga tggcatcgtt aaggcagcca ttggctcgct tcagacccag attgccggac
    27901 aggccggccg tatctcctcg gagcaggatc ggatcgacca gatcgagcag ggcctccggg
    27961 agaggatggc cgccgcggat gcgctgattg ccgcgctcga gcggcaggtc ggctatttca
    28021 ccagcctgtt cgaatcgatg cgcctggcgc gggagagccg gaggtgacgg aggccggcgt
    28081 agtggaggcg cggttccggg aggccgtggc ccgtggggac tgggcggagg cgcggagatg
    28141 cctgccggag tacggacggg agattgaaga acggcttcgc aacctcccgc ccggcgagga
    28201 tgcggcggct ttgctcgggc gggcggtgaa gttgctggag caggtccgcc gggcggccgt
    28261 ctccgagcgc gcacaagccg gcacggaact ggagcgcctg gcggcggcgg tctcttaccg
    28321 gcgtgccggg cacgaaggct ttcgggcgac actcgaactc gaaggctgaa acgcccgttt
    28381 tctgtcgccg gcaattttct gtcattcctt ccttcagatg gttccttact tttctgcggg
    28441 ttcgcagtgg tccgtttcgt gccttggatc tggcgtgatg ctggatcgtc tcggcggccg
    28501 cctggaacag tacatgaacc tgttgagcgc gcgccagcgg caggtcgcct caaacatcgc
    28561 caacgccgac acgccgggct acaagacccg ggacatcaac tttcaggcag aattccgaga
    28621 cctgatggag aacgggtccc cgcgcgcggt ggaggtagcc ggcttacagg cgaagaacga
    28681 cggcaacaac gtcagtctgg accgcgaggc gcgcctgctg gcggagaacg cgctacgatt
    28741 cagcgtcgcg acgagtcttg cccgcgcgga gatccgggct gtgaagtcgg cgattcagga
    28801 agggaaaggc gtatgagcct gttctctctg ctttcagtga gcgcttcggg gatggcggcg
    28861 cagaggacgc gcgccgaact gctggtggaa aacctggcca acgcggagac gacccgcact
    28921 ccggaaggcg gcccataccg ccggaaagac gcggtctttg cagtcgacgg agcgccatcg
    28981 ccattcgcgt cggtgttcca aagcgtgtcg ggtgcgccgg acggagtgaa ggtggaggag
    29041 atcgttgtcg acacacgcga gccggagcgc cggtatctgc cagggcaccc ggacgcggac
    29101 ggcgaaggtt atgttgcctt cccacgggta aacgccgcgg aggacatggt ggacctgatg
    29161 ggcgcgtcgc gcggctacca tgccaacgtg tccgccatgt cggcggtgaa ggaaatgatc
    29221 catcgctcta tcgatctgct gcgttaacgg agtaagcgga atggtcaact cgatttcccc
    29281 gatccgaaat gtgtcgttgc ccgagctggc ggccccaccc gggaaggaag gtgcatccgg
    29341 cgcgtttcgg agcctgctgg agggagcgat cgtgccggtg gagaacgcgc gcggactggc
    29401 ggccgattcc gtggagaagt tcctagcggg cgagggcgaa gaacttcatt cgacggtact
    29461 ggcggtgcag agggcggaaa tggcgctcga cctgttcctt caagtgcgca acaaggtggt
    29521 tcaggcttat caggaaatca tgaggatgca agtctgagcc cctcggggcc cggccgaacc
    29581 cgatgtcgca atggaaaaag atcctggcct cgttctcgac gcggcagcgc gtcgcgatcc
    29641 tgctcgcggt ggcagtcgcc ggcgcgggcc tgtgggcgct gacccgatgg cgccgggaga
    29701 ccgacttccg tcctttatat acggcactcg aggcggacga cgctgccgcg atggtctcaa
    29761 ggctgaaaga aaccggtgtc gagtaccgcc tttccgagga cgggcgaact attctggcac
    29821 cgtcggcgaa agtggccgag gcacgccttt cgatggcggc agcgggcctt cccaagacag
    29881 ggcgcatcgg cttcgagctg tttgacggca cgaaattctt ggcgaccgag ttcgccgagc
    29941 acgtcaacta cgcgcgcgcc ctcgaggggg aactggaacg atcggtgatg taccttgcgg
    30001 cggtggagcg cgcgcgcatt cacctgacgt tcccgaaaga ctcggtgttt ctggaggcgc
    30061 ggcagccggc caaggcgagt gtgatcctga aagtgcggcc tggagcgcgc ctcacgccgc
    30121 agaacgtcgt cgcggtaagc caccttgtgg cgagcgccgt cgagggattg acgccggatg
    30181 cggtatcggt gctcgatatg caggggaacc tgctgagccg tccccggagg atgttgacgg
    30241 ctgacgggat gggggcctcg gacgggatgc tcgaattccg gcagagcgtc gaaaaagacc
    30301 tgctggcgaa gatccacgcg acgctcgaac cgctgggcgg tccggagaag ttccgcgcaa
    30361 gcgtgtcagt ggattgcgac ttctcgagcg gcgagcagag cgaagagacc ttcgacccgg
    30421 cgcgttcggt gatggtgact tcgcagaaaa ccgaggacgt cagcggaggc gcgggtagtt
    30481 ctggagtccc cggtacggcg tcgaacctgc ctcggccgag ctcgcgtccg ggaagctccc
    30541 aaaccggact cacgcgccga acggagaaca tcacctacca gtcgagccgc gtggtgcgga
    30601 ggatgcggct gccgctcgga aatacccagc ggatgtcggt ggcggtcatt gtcgatcagg
    30661 ctgtccgatg ggaaggaacg ggggcgaagg cgcgaaaggt gatggaaccc ccgacgccgg
    30721 aacggatgaa gtcgatccgc gatctggtgg cgggagcgat cggcttcaat gccgcgcgcg
    30781 gagaccggct catcgtggag agcctgccat tcgagtcgac gctccaactg gagcctcccg
    30841 agggcgcccc cgcgccatcg cccgcgtccc cgctgtcagc gtggctggag atccttcgga
    30901 aagcgggcgc ggcggcgatc ctgctgctgg cgctgtccgc cgccgtggtt ctggcgcgga
    30961 gaaagcgccg gcggaaggtc cagatttcgt caccccggga gctttccgcc acgccgggcg
    31021 agaagccgga cggcaagtcc atcgccggag tggtcgaaga ggcgaagcgg atcgagcaag
    31081 caagcggcgc gctgctggag tcgccgcccg cgccgaccaa gaaggcggat ctgctggcgg
    31141 gacggctccg ggacgtcatc aaggcggatc ccagcggtct ggcgcaggtc atgcgcacct
    31201 ggctcgcgga agaggaggag taggcgcacc cggcgatgat tctcaactcc cccaaagcgg
    31261 cgccggggat tccgggagcg caaaaagcgg ccattctgct aatgatgctc ggcgaagaga
    31321 cgggcgccga gctgatgcgg cagctcagcg aagacgatgt ccagttgatc agccgcgaag
    31381 tcgcccggct gggaaccatc gctcccgacc aggcagaggc ggtgctggat gagttccacc
    31441 agatggcggt ggctcgggac tacgtcgtta agggtgggtt cgaccatgcg cggcggatgc
    31501 tggtctccgc gttcggtccc gaggcggcag agaagatcct ctaccgtctg acgcggacac
    31561 tccacgccga tatgacggat ttcgccgcgc tgcaaaaagc cgatcctcag cagctcgcca
    31621 agttcgtggc cagcgagcat cctcaaaccg tggcgctgat cgtttcccag ctcagctcga
    31681 cgcaggcggc ctcattgttg atgtcgctgc cccctgagat gcggtcggac gtgacgctgc
    31741 gcatggcgag cctcgaccag atctcgcccg agatcatcaa caagatcgca gggatcgtgc
    31801 accagcggct gaaagggctc ggacaaatca gccggcaatc ctacggcggt gtgcgcgcgg
    31861 tggctgaact gctaaaccgg ctggattcga gcgtcaccaa ggagattctt gagagcatcc
    31921 atcagcagga ttccgagatg acggagaaga tccgtcacct gatgttcgtg ttcgaggacc
    31981 tgctgctgct cgatcagagc ggggtgaaag agatcctggc gaaggtcgac cgcaaactgc
    32041 tgacgatggc gctcaaaggc accagcgaat cgcttcgaaa ccacgtggtc caatgtatgt
    32101 cgcagcgcgg gcgggaaatg cttctggagg acatggacgc cctggggccg gtcaagatca
    32161 aagaagtgga ggctgcgcag caggccatca tcgcggtggt ccggcaactg gagagcgagg
    32221 gcgtgctgag tttgaaggaa acggccggcg agcagtatgt cgtctaggat cctgcgggaa
    32281 ccgggacccg gccgagccga gccggtcgtg tggccgagcc tcacggcgcc ccagccggcg
    32341 ccaacccccg aaccaagcac cgaaaaggcg cccgatccgg ccgaggagct ggatcgccgg
    32401 ctccaggaag cgcatcggtc aggctatcgc gaaggcgagg cagccgggcg ggcccagctt
    32461 gcaccggtgc tggagcaact cgccggtagc atcgagtcgc tcgcttccct gcgcccgctg
    32521 ctgcgtaaga aggccgagtc ggacacggtg cagctggcac tggcggtcgc gcgacggatt
    32581 ctccgccgcg agttgacggt cgatcccgag gcgctggagg gcctcgttcg agccgcattc
    32641 gagaagctcc aaggacagga gatctgccgc gtgcgtctgt ggccgggtca cgaaaccgtg
    32701 gtgcgggagt ggctcgatcg gctgggtgcg ccgcagacca tcgagatcat cgcggaccgt
    32761 tcccgccatc caggcgacgt ggtatttgag accgcgcgcg gtgcgctcga cgcctcggtg
    32821 gagagccagc ttgcagagat cgagtacggc ttggcggacc gcctgcggag gagaacgtga
    32881 gtgtgccggc acagatcgac ctgagcccgt acctgcggga cgtcgatcac gtcgaacccc
    32941 tgcgctggtg cggggaggtg gtggaactcg tcgggctcct ggtcgtctcg cgcggtccgg
    33001 ttgcggctgt gggggatctc tgtgagatcc ggacggtatc cgggagcaga atacggacgc
    33061 aggtgatcgg tttccgtgac gggcacgttc tgtcaatgcc gctcgaagag accggcggcc
    33121 tgcaactcgg ggacccggtg gtcgctcgcg gcggcgaggc gagcgtaggc gtcggtcagg
    33181 gccttctcgg acgggtactt gacggattcg ggcgccccat ggacggcgga ggcgaggtgg
    33241 acgccgaggc cgagtacggg ctttacgccc agccgcccaa tgcgctcgag cgcgagcaca
    33301 tcgaccagcc gctggtgacc ggcattcgca caatcgacag cctgctcacg tgcggcaagg
    33361 gccaacgcat cgggatcttc ggaggcagcg gcgtggggaa gagcacgctg ctggggacga
    33421 tgtcccggca taacttcgcc gaagtgagcg tgatcgcact gatcggcgag cgcaaccgcg
    33481 aagtaagagc gtttttggaa catgaactgg gcgaggaggg acggcggcgg tcggtcgtag
    33541 tggtggctac cagcgaccag ccggcgccgt tgcgcgtgcg cgcctgcttt gtggcgctgg
    33601 cggtctccga atactttcgc gatcggggcg ccgacgtgct gctggtgatg gactcggtga
    33661 cccggctggc gatggcgcag cgcgagatcg gcctcgccgc gggtgagccg ccgagccaaa
    33721 aaggctacac gccctccgtc ttcaacttgc tgccgaagat cttcgagcgc gccgggaggt
    33781 tccggcgtgg atcgatcacc gggttcttca cggtgctggt ggaaggcgac gacttcaacg
    33841 aaccgctctg cgacgccgtg cgcgccaccc tcgatggcca catcgtattg tcgcgtcagc
    33901 tcggcactgc cgggcactac ccggcaatcg aggtgctgaa ctcggtcagc cggctcacat
    33961 cgagcatcac cagccgcgag cacgctgagt gggcccgcaa agtccgggaa gccatggccg
    34021 cctaccagca ggccgaggat ttgatccagc tcggtgccta tgtgggggga accaatccga
    34081 aactcgacac gatcctcgaa atgcgggagg aactcatgga gtttctgagg caggacgcct
    34141 cggtgaagac tccggcaagt gagacgctcg cgcggctcga ggccttggcc tcgaagctgc
    34201 cctgatccgg cgaggagcgc atgaaggcgt tttatttccg gcttgagagg gtgctcgaat
    34261 ggcggcgaac ggaattcgag ctcgcggagc acaggctgcg cgaggccgcc gcggccctcg
    34321 gccgcctgga cgaggaaagc cggcgtctct cggccggaag agaggccgcc gagcgcgccc
    34381 tgcggacggc ggactctgtg gacggcgccg atctcggtgc ccattccagc tatctgcggc
    34441 ggctgcgccg gcacgccgac gcgctggccg cgcaccgcgt ccagctcgac cgggagttgg
    34501 cggcgcggca gcagcgtctg attgaggcgc ggcgccagct gcggatcatc gagaagtacc
    34561 gcgagcggcg attggttgcc tggcaagccc aactcggccg cgagatcgaa gcgttcgccg
    34621 gtgaatcgta cctcgcgcgc tggaattccc gccaatccgg ccgcaaaatt ccaccagcag
    34681 gccgataagg cctgtgtgag cgaattcctt cgaaacgccg agagcattct ggacggagcc
    34741 gcggccgcgc tggcatctgg agggcagcct tccgagatgg tgattctggt gggccgcgac
    34801 ggtgtcatac ggatcgtcgc ggagccggac tggccgctcg acgcgctcga agcctaccac
    34861 agcgcccgca tggcttaccg cgtctcgggt cgtgacgggc gggtgcgcgt ggagggccga
    34921 tgcggtgggg cgtcctgcat gattgtcgcg gaccctccgg cctcggtggc gcgacggttg
    34981 ctcgccggtc acgcctgggc gccgaacgtg ccgttggcgt gtcttccgcc cgccgcctcc
    35041 tgatttcttt caacttttcc cgtcgatttc cgataagtcc tgacgaggcc ctgcgccgag
    35101 gcccgctttc cgccacgaaa cggacggctc caggaccctc gaggagcatg gtatggacat
    35161 cactccgatg caccggccag gaagcgccct ctcgccggct ccggcgccgg taccggcgga
    35221 aacggccgtc gagaatcgcg agatcattca ggcggtgaaa gccctcaacg ccgccgaact
    35281 gttcggacag aacagcgaac tgacgttcct gctcgaccgc cggacccaga ggcccgtcat
    35341 ccgtcttgtc gaccgtaaga ccaaagacgt aatccgccag attccgccgg agcacgttct
    35401 tcgcatggcg cgggatcttg cgcatgcgga gtaacgtgcc tgcaaacagc gcttgagaag
    35461 tcgtgtttcc ggccgataag tgcactggag ggaacacgat gcccgcgagc gcccatgacg
    35521 cctacctcga aagcacgatc ctgagcgcgg acccggtgga actcgtccgc atcctgtatc
    35581 gcaccgcact cgatgcggtt cgggaggcaa gggttcacct tgcgagcggg gatatcacgg
    35641 cccgagggaa aacggtctct cgcgcgctcg acgtcatcaa cgagctgaat ggatcgctcg
    35701 accaccagac cggcggcgcg ctcagccggc agctcagcga gctgtacgac tacatgcaga
    35761 ggcgactgat cgaagcgaac taccggcagg agacagaggc tcttgacgag gtgtcggcgc
    35821 tgctcgctac gctgctcgaa gcgtggcagg ctgtcgggac ctccgcgcag cccgaggagt
    35881 cgcgatttga agcggacctg aggcagatgg cttctctgcc ggagttccag gccgagatgg
    35941 aatacgcgcc gcaggcctgg agcgcgtagc agccccaagt ggcgtggcgc cggcggggtc
    36001 agtcctttcg aagcccgctg gcgacaatcc tggccaaccc gaggccgccc tgggccgcca
    36061 gggactgcgc gaactgttcc tcggcaagct ccatcgccga cgacgaggtc gagtcgtcgt
    36121 cagtcccaag ccatccgggg ccggcctcgc gcatcgactt caatagttga gcgatcagca
    36181 gcgcctcgaa ctgtcgggca gcgtcttcga tccgttcagg tcggctgtca ccctccggcg
    36241 gggcgttggg cgaagggcgg atgccgccga cggtcatcag atgacctcca gctccgcttc
    36301 gagcgcgccc gcgccgcgaa gactttgcag gatggcgata atgtcgcgcg gcgtggaacc
    36361 gattgacgaa agcgcccgga cgagttcctc gacagttgca ccctgcttga gcacgaggtt
    36421 ccgggctttc tcctccttga ccccgacgcc gacctgcggg actacttcgg tggttccgga
    36481 ggagagaggt gcgggctggg acaccgcgaa gctggtctcg atttcgatgg tgaggttgcc
    36541 gtgcatgatg gcgaccggag aaatccggac ttccttgccc atcacgatcg tgcccgtgcg
    36601 ttcattgacg acgacccgcg atggccggtc ggcctcgacg ctcagccgct ccagttcggc
    36661 gacgaattcc gtggggcgcg cggcatagtc ggccgggatc tccacccgga tcagcgctgc
    36721 gttctcggcc tgcgctgtcg gcttggcggc gctcccgaaa ctcttgttca cggcggcggc
    36781 cacacgcgcg gcggtcgtga aatcgggctg gcgcaactgg agacggacac ttcccttggg
    36841 cgccagcgac ggtgccgccc gctcgacgat cgctccgttt gggacccggc cgacggtcgg
    36901 gtggttcaag gtctggttcg taccctggcg tccggccaca aaaccgccgg tcaccaccgg
    36961 tccctgcgcg accgcgtaga cttggccgtc cactccgcgg aggcttgtca gcagcaggag
    37021 cccaccctgg aggttcgacg cgtcgccgat ggcggcagca gtaatgtcga tccgtgtgcc
    37081 ggtctgggcg aacgccggca gcgtagccgt gaccatgacg gcggccacgt tattgacgcg
    37141 gatcgcttcc ggcgacaccg acacccccat gcgctcgagc acgttggtca aactttgcgc
    37201 ggagaatacg gtctggcggc ggtcgcccgt gccggcgagg ccgactacaa gcccgtaccc
    37261 catcaattgg ttgtcgcgca cgccctcaag cgtggccagc tccttgagac gtgtttcgcc
    37321 gggacctgcg ccgagcatac ccgccaggag ccaggccgag attccgaaca gtcgcctcat
    37381 cacggcagca accccagcaa cagcctgtac aggaagaacg ggcgccgcac ggcgtcgccg
    37441 acgacgccct tgccgttgat gcggacctcg agttgcgaga tccggtcgga ccggacgatg
    37501 ttaccggggt tcagatcctc cgggcgtacc actccgcgca ctgtgacggt ctgctgttcg
    37561 gagttcacct ggaggtcctt cgtgcctgcg acgacgagca gtccgttcgg cagcacgtgt
    37621 gtgacgcgcg ccgccagcgt cgtgctgaga agtgtctcgc ggctggtcga gccttcgccg
    37681 ctcaactgcg actcgccgtc caggttcgtc aggttcgcca gcggaccggc cgctcgcgtg
    37741 attccgccga gcgcgctgat cgaattgcga ttgctcgata cgcgcgccga tttcgtggag
    37801 cccttggcca ccgctgaggc gcgctccatt acgagaatgg tcacgacgtc atccaccgct
    37861 cccgcccgga cgtcccgcgc cgggtccgcc agtttcgcgg aagaagacca gaccgatcct
    37921 ggcgaggcgc tctgtctccc cggtccgtcg cgtgccatcg cctcttccac gtagcggtcg
    37981 agcggtgatg gcgcggggct tcggtctttc tttgcgggga gagcgggaag cgccaccgcg
    38041 gcgagcacca gcagacaaaa gcagttcctc cggatcatcg cgtcggcttc ctttcgtccc
    38101 ctgcggcaac gagcccacgg ccgatcactc tggcacggaa tcggcggccg ctttccggat
    38161 tgcggaccag aaccgtatca cccttccgtc cggaggtctc agcccggccg tggatctcaa
    38221 tccgcgcggc gccgctggcg gcctcgacac ggaccatctc gcccccggcg acctctctcg
    38281 gcgcctcgag cgctccgaga ggcaccggtt gtccggcggc gatccgccgc cgcggtgtac
    38341 gtccaaccgc ctgttccggc tgtgccgcgt agatcgccgg ccgtggatcg gcggaatagg
    38401 tctcctgctt cacctgcgcc gcgccgatgg gacgtccgac aggcagatcc tcggtggcaa
    38461 tcagccgttt tgcgggaatg gaaatacgca cccgcgccca gatccggaag cggcgcttgc
    38521 cgtcgtggag caccgagccg cgccagacgg ccggcgtctc ggggtcggag gacctcgaca
    38581 ggcccgagac cgggaactcg acctccccct tcggcgccgg atagcggctg aagtcgacca
    38641 gttcgatctt cgcctccggg cgccccagtg ccctggtcat cgcgtcgagg aggcggtcgc
    38701 ttcggagcgg ttcgagtaga cgttcgatgc agatctcttt cgagggatcg acatcgacgg
    38761 agtgacgccg gccgaggcgg gccagttccg cacggcgcag gatccgccta gccccgggcg
    38821 ccggcgcgaa tccgagcggc gtctccgcgg ggagacgcgc gaaggccggc tcggccccgg
    38881 ccaggtcagc ggcgacaatt tgctcgcctt cgaccgcgag acatcctgaa accagtaagg
    38941 cggcgagcag cgtcatctgg tgaggttgtt gatctgctgg tacatgtcgt ccgccgcctt
    39001 cacgactttc gagttcgctt cataggcgcg ctggctgacg atcaggttga tgaattcctc
    39061 caccaccgag acgttggact gctcgagata gccctggagc aaagtgccga tgccctcctg
    39121 gccgcccgga ttgccgatcg tcggatctcc cgaggcgtcc gtcggcagat agaggttttt
    39181 cccgatgctg ttgagcccgg cggggttctg gaagttcgca agctggatct gcccggcaag
    39241 ctggatcgcc gtctggccgg gttgcgtgaa gctgaccgtg ccgtctggcg cgatgctgat
    39301 cgcctgcgcg tccggcggaa tcgtgatctg cggctcgagc gggtcgccgt ccgccgtcac
    39361 gagatttccg tcgcggtcca ggtggaacga gccggcccgc gtgtacgcga tttcaccaga
    39421 gggaaggcgc acctggaaga agccgcggcc ctggaccact acatcgagtg gattctccgt
    39481 ggccgagaaa ttcccctgag aaaaaaggat ctcgttggat gcagctcgcg tgccgagccc
    39541 gagctgaaga ccagtcggca ccaccgtctg ctggcccgcc gaggcgcccg gtgtcaccag
    39601 gttctgatag agcagatcct gaaactgcgc ccgccgtacc ttgtagccga tggtgttggc
    39661 gttcgccaga ttgtgcgcga tgttgtccac gttcaactgc tgcgccgtca tgccgctggc
    39721 ggcgctgaat agcgctcgaa tcatgatttg ttctcctctc ctcccgggcc gcgtcaggag
    39781 ccgacgcggg cgacctcctc caccaccttg cgattcatct ccccgccaat cgagaccgcc
    39841 ttctggagca tctcgaactg cctcatcacg ccgatcaggc gcacggcgcc ctcggcgccg
    39901 gtgacgttcg atccctcgag cttgccctgg tgcacggccg cccccgtggc ggcaacgggt
    39961 tgcgcccgcg gatcgtcggc gcggaagtag gcgcggccct gttttgccag cgctcctgtg
    40021 tcggtgaagt cgacgagttc caactgccct agcacctggc cgtcctgcgt caccacgccg
    40081 tcgggcgcaa cctcgatcgg actctgtgac gaagcccgca gcgtcccgct acccaccgct
    40141 cgcaccggat agccctcagc ggtcgacagt acgcctgtgg ccgacaactg aaagccgccg
    40201 ttgcgcgtgt acagccgccc agccgggccg tttaccgcga aaaagcctcg gcccgagatc
    40261 gcaacgtgaa aggggttgcc ggtcgggtgc agcacgccct gggtaaagtc cgtccaatgg
    40321 cgttcgatca ccggcagcgt ctgtgttatg ccgcctcccg ccgggtcgag agactccggc
    40381 gaaacgtaga gaccatagaa ctcgcggtcg gtcttaaagc cgtcggtcga gacgttagcc
    40441 aggttgttgg ccagcatctc gagcgattcc atccgcgccc gcaggccgct ggcgcccgaa
    40501 atcgtgagtg ggtccatgac gcgctccgcc gcggtgtcgt gcaattgcgg gaccgaaggc
    40561 gccggagccg gcaaatgtcc tcaaatgttc gtggaagccg ccgatgaagg aaaagggagg
    40621 agcatgccta cgagccaatt tcttgcctct tcccgccagg cggcaagttc ttagcggtaa
    40681 gtgtcccgct aacccgttga aatcccggct tccgaatctg gcttttcatc tgcttctacc
    40741 ggcgcgtgac cgtttcgtgc ttccctctgc cgctcgccgg gacgattctg gatgtgcccg
    40801 ccgcagtgga gggaaccgcc accgtgacgc cgcaaggtgc caactggatc gacccggacc
    40861 ccgccgccgg tgctgctttc gggcagcttc ttggaacagt gcttgagact ttgagcgagg
    40921 aggggatcgg aaccggaacc ggaaccgggc cgtccatcgg gcgcccgccc attccgatgc
    40981 ggaccgaaac cgtaccggag ccagccgaat ccggcgacgc caagccggag acgccggaga
    41041 cggatcccga ccccgccatc gcagtggccg tcccggtttc gggcgtgctg ccgttcgccg
    41101 aaccgccact cgacgcacaa ccaacggtcc agccttcccg gctggccccc gagaagccgg
    41161 cttccgattc ctggccgttc gacgtggcac atcgtgtctc tctcccggac gctgcggcag
    41221 gcgacgagtc ctcggacctg ccgcccgcgt cttccggcac acgagacgtg ccggcgttca
    41281 gcccggcctt cactgaggac cccggaaacg cggatgtccc cgaaacggct ggcgtggctt
    41341 ccctgccgac agtccgtccc cctgcctcct ccgggccgtt gaacgttgaa ccgccggtgg
    41401 aatggccgcc acagaaggac cgtccgccgg agtcatcggt tcctccgatc gtcacggcgc
    41461 ctgcggtttc gtttgattcc cagccgccga ccgaaaccgg acggaccgcg gcccagcggc
    41521 cggcatcgcg tccaatgata acggtgcgga cagcgccggc cgaggagccg ccggtggcgg
    41581 atgcgaaacc tgccttctcc atgcgcatcg tgggagcgga tcgcgatcaa acggcggaca
    41641 cggaggcgct acttgcaccc ccggaacagc cggccggcgc gaaactccgc cgcgcaaccc
    41701 aagtggatac gctcacgctc ttaccggagg acccgattgc catcgcggca gcgccggcgg
    41761 cgagacccgg ccgacctgtc caggcggaaa cggtgcgggc tgatcagccg gaggctgaaa
    41821 tcgacagaac ggagccgccg gaggtggcgg tgcggcggat gcccgagaca taccgaggag
    41881 cagccgcgcg acaaatctcg ttgcgtgtgg ccgaaggcgg ggaagagcgg gtcgaagtgc
    41941 gcgtcgcgga gcagtccgga gaattgcggg tcgacgtgcg cgcccggaat gaaggactgg
    42001 ccggccgtct gcgcgacggt cttcccgaat tggtcgggcg gctgcaggaa aacgggctcg
    42061 aggccgaggt ctggcggccc tccgggatcg atgcgtcgaa gacccctgca cgacgggacg
    42121 agctgccggt ggcaagccag gagaggaatc ccggcggcga gcgcggctcg ggctggcagc
    42181 aaggagagga aggcggccgc cggcgccatc agcaggacgg ccagagccag agtgaggtct
    42241 tcgatccgta cttccaacta cggaatgaga acggacagga ataaagcgca atgacaattt
    42301 cgagcgatcc gaacattcgt acggccgccg attccggcgc aaccggagac ggctcggctt
    42361 cggcggacaa caaggacgtc agccaggtcg ccaccaagga cgccttcctg cgtctgctgg
    42421 tggcgcagat ccgaaaccag aacccgttga accctgccga cggtgtccag tttctgacgc
    42481 agctcgccca gttcagtgag ttggaacaga tgatcacgat gcggcaggaa gtggaagggc
    42541 tgcgcgacga cattcaggcg gcgctcaacc cggccgcgga cacgccggcc agcaccccgg
    42601 cgtagagaac aaaaggagat cagatggcat tcacggcatt ctcgacggcg ctctcttcgc
    42661 tgagtgcgca cgcgacggca gtcgacgtcg tcggcaacaa cctggcgaac ctgagtacgc
    42721 cgggcttcaa ggcgagcacg gtggtcttcc acgacctcgt aacgcaggcg ctcggtctgg
    42781 gtgagactca ggtcggtttg ggtaccgggc ggccgctgac catccgtcag ttctcacaag
    42841 gcgcgcttca atcgagcggc ggaaatctag acgcggcgat tcagggagac ggtttcttca
    42901 ttgtccgcga ctatactgga gcgacgctgc tcacacgggc gggcaatttc cgggtggacg
    42961 aggaaggcaa cctgttgacc gcgaccggcg accaggtgca gggatggacc gagagcggcg
    43021 gcgtggtgga cacgaacgct ccgatcggaa ctatcgtcgt accggtcggc tcgttgcgcg
    43081 cgccgtccgc taccaccgag ttctccctca acctgaatct gaacgcgagt gcggtcgtcg
    43141 gcgaaccgtc ggcatccttc tcgactccca ttgaggtcgt ggactcgatc ggtgctaccc
    43201 acgtggtaac ggtgaacttc accaagacgg ctgccaacac gtgggactac gaggtgacaa
    43261 tcccgggcgg cgaagtgacc gcaggaacgc cggggactcc ctactcgctc ggcacagaaa
    43321 ccctcacttt cgacgatcac ggcctgctgg cgactccggc cgcgccggcc acaatcgctg
    43381 tcccggtggc cggcctcgcc agcggagccg ccgatctcgc catcgactgg gatctgtatg
    43441 accccgacgg cacgtcgcgg ctcacgcaat actcgcagac ctccgcggtg gcggccgtgt
    43501 cgcaggacgg cgcgccggcg gcgcaactgg tcaaggtcgg ccttggcgcc ggcggcacaa
    43561 tcatggcgca gtactccaac ggcgaacaga aggtcgtcgg ccaaatcgcg ctggcggccg
    43621 tgcgcaatcc ggattcgctg atcgcggtcg gaaacaacaa ttacatccag acggcgcgta
    43681 ccgcgtctcc ggcgatcggg atgccggaaa cgggcggccg cggcaagatc gtcagtcagt
    43741 cgctcgaagc gtcgacggtt gatatcgccc aggagtttac taatctgatc gtgttgcagc
    43801 gcggctacca ggccaacgcg cgggtggtga ccgcggtgga cgagattagc caggaaacga
    43861 tgaacatgaa gcgctgataa aggaaggccc cggcccgatg acgcccatgg aggagatcgc
    43921 agccttcgcc gacgtgccgg tttcgatcga agtcgaactc ggacggccgt tgatgaagct
    43981 gcgagacata ctcgaactgg aaacgggaag cgtggtgtgc ctgacccggg cggccggtga
    44041 aaacatcgac gtgcgcgctg cc
//
LOCUS       DATIFQ010000402         8654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1092975, whole genome shotgun sequence.
ACCESSION   DATIFQ010000402 DATIFQ010000000
VERSION     DATIFQ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092975"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VLE22_19680"
     CDS             complement(<1..1020)
                     /locus_tag="VLE22_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16682.1"
                     /translation="MGWFRFLLLPLVTLAPAAAQVDVVTANYDKDRTNANLHETILNV
                     SNVNVARFGKVFSLPVDGFIYAQPLYVRNLTIPGKGVHNVVFVATMHNTVYAFDADAP
                     QAPLWTVTLGPPVPSSDYELEDIVPEVGILSTPVIDLDSGTIYVVANNIENGLYFFRL
                     HALDITTGEEMFGGPADISASVPGQGQGNQDGMVSFVTFDHLQRPGLLLLNNVVYASF
                     GSHQDRPPYHGWLIGYDARNLQRQAAVFNVTPDGEEGSIWQSGRAPAADNFGNIYITT
                     ADGDHNGAANWGESILRLGAAGGLTVADWFTPDNWMDLNLHDDDLGSCGPVLIPGTDL
                     LVSGSK"
     gene            complement(2170..4380)
                     /locus_tag="VLE22_19685"
     CDS             complement(2170..4380)
                     /locus_tag="VLE22_19685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HSB16683.1"
                     /translation="MAASILRSLLASNALAMTLVAGGLWAQPASITTTATTVPVAGPA
                     VFDPAGNLYFFLAGPVTPGAAQTQNGGGTCLSSNGFFSFPGPCPDGYAGKLDASGNLV
                     FGTYLGGPTADQSTALAVDGAGNVFVTGSTEGSFPTTAKAAIAASTTAKSFAAKISAD
                     GSRVLYSTYLPDTAGMPSAIAIDAQGNAYIAGKSSTGHAFVVKLSADGSAFLYHVSLA
                     GSAQDSVNAVSADSAGNVIVAGQTTSPDFPVTPGTVQSRLMGAQNGFIARLDPSGGVV
                     FSTYLGGSGTDTPTAVQTDSASNIYVAGQTSSLDFPTTSGSFEPDPIVPMWNNSAPAG
                     FAAKLSADGSALSWSSYVMSADHPLYQGVTRPSLGVAQLAVSASGEAYIAGLTGPGFP
                     VTASAPQVCFDGLHSAQFYNGIGATDAFVAHLDPHGALLDATYVSQGVTFTVGLSLAG
                     DGSVLLAMDSAGNRVKSRLRFGGTGWKAPACLSPAVLNAATLSTIPYSATGPALVPGE
                     FITLTGFGIGPDIGVADQGQVTRELAGVQVLFDGQPAPVLYAQSRQINALAPVELSGQ
                     TKTNITVLYNRAAVGSIEASVVSYGSPGIFRLHPGVSSQAAAVNEDGTINGPANPAAR
                     GSVVSVWGTGFGLIDPPCATGGLNPPGPVSLAAGISVDIADGSPPGVPVQYAPALYAG
                     SAPTLPCGVVQINVQVPTYVAPGLYQFFPWSLMALPGGAQSVAIGTIGATISVK"
     gene            complement(4567..5439)
                     /locus_tag="VLE22_19690"
     CDS             complement(4567..5439)
                     /locus_tag="VLE22_19690"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HSB16684.1"
                     /translation="MKTGLRELGVSNVGVALFVISIALGGAADAATGRHPILIPCAAI
                     GLYLLFSIRVADQWEKFAVLRFGRYIGLRGPGLFHIIPIVDTVSRTVDQRVRVVSVSA
                     ESTLTRDTVPVNVDAIVFWLVWNAEKSILEVQDFEQAISLSAQTALRESIGRHELHQM
                     VAEREMLGQELQRILDEKTNPWGITVQSVEIRDVQIPSQLQDAMSREAQAERERRART
                     IISLAETEIADRFAQAADTYRDNPVALHLRAMNMLYEAIKEKGSMVIVPSSAVETMGL
                     GGTLATAALGGAKP"
     gene            complement(5436..5861)
                     /locus_tag="VLE22_19695"
     CDS             complement(5436..5861)
                     /locus_tag="VLE22_19695"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSB16685.1"
                     /translation="MASTVQDSKRPFGFRLDLKSGVPVYRQIIDQVTGGMAAGALKPG
                     DQLPTVRQVAVDLAINPNTVVRAYRELEIRGLLETQQGTGTFISRQKVKGDQVERQRR
                     LNQLVGEFVARAGAGGFTIQDLVEQLRDLQADQERKVAP"
     gene            complement(6082..6549)
                     /locus_tag="VLE22_19700"
     CDS             complement(6082..6549)
                     /locus_tag="VLE22_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16686.1"
                     /translation="MQVRQTVALCVAFVLFAELIVAAVRGDEVMYIGGTVKNLAEATE
                     GRLDTQNEEKLIFTSDKGGFEIPYGSITSLEYGQKAGRRVGVAIVITVWALFSKKRKH
                     FLTIGYNDSQGKPQGVVIEIAKGRAKTMITIIEARSGKKVEYESEEAKKHVHG"
     gene            complement(6555..6977)
                     /locus_tag="VLE22_19705"
     CDS             complement(6555..6977)
                     /locus_tag="VLE22_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16687.1"
                     /translation="MPTLALAAVRGDEAMYVGGTITSIPEKTEGRLLLDDSAALKFTS
                     KKGDLSITYTKISSIEYGQKAGRRVGVALAVSPLALFSKKRKHYVTIGFADEAGKNQG
                     AVLEVSKGNVRSLLTTLETRSGKQVEFESEEAKKNAGR"
     gene            complement(7257..7604)
                     /locus_tag="VLE22_19710"
     CDS             complement(7257..7604)
                     /locus_tag="VLE22_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16688.1"
                     /translation="MNQRPLSVTVFGFLYVAAGALGLAYHLTEFKTQHPFPYELVWVS
                     VVRLIAIVCGVYMLRGSNWARWLALAWIAYHVILSGFHSRFELVMHSLLCAVFAYFVF
                     RPRATQYFRAVRT"
     gene            complement(7686..8321)
                     /locus_tag="VLE22_19715"
     CDS             complement(7686..8321)
                     /locus_tag="VLE22_19715"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:NF003768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HSB16689.1"
                     /translation="MKNVLADEALDQLFREARTHSAWLDKPVTDDMLRQLYGLMKWGP
                     TSANASPARFVFLRSREAKERLRPALAPGNVAKTMAAPVTVIIAYDLLFYEKLSKLFP
                     HNPAMCALFAGNPQLVEVTAKRNSSLQGAYLMIAARALGLDCGPMSGFDNARLDEEFF
                     GGGKECEGCEQEFFPAGHVKSNFLCNLGYGDASRLYPRAPRLEFDEACTLL"
     gene            complement(8384..>8654)
                     /locus_tag="VLE22_19720"
     CDS             complement(8384..>8654)
                     /locus_tag="VLE22_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16690.1"
                     /translation="THDHLKREAARPKPCAPFCTISCVHQTAMLDAFREDPHSTLAGM
                     IERRKAHNPAYQPPLMLKALTWAFLDGRTRRVLSRLALHVLGARS"
BASE COUNT         1658 a         2590 c         2643 g         1763 t
ORIGIN      
        1 cttactcccg gagacgagaa gatcggtccc agggatcagg acgggaccgc aagagccgag
       61 gtcgtcgtcg tggaggttca agtccatcca gttgtccggc gtgaaccagt ccgcgacagt
      121 aagtccaccg gcggcgccga gccggagaat gctttcaccc cagttagcgg cgccgttgtg
      181 gtccccgtcc gccgtggtga tgtagatgtt tccgaagttg tccgccgccg gagcgcggcc
      241 cgattgccag atggatccct cttcgccgtc cggggtcacg ttgaaaacgg ccgcctgcct
      301 ctggagattc cgcgcgtcgt aaccgatcag ccatccgtgg taaggcggcc gatcctgatg
      361 cgacccgaag gaggcataga caacgttgtt gagcagcagg agacccggcc gctgaaggtg
      421 atcgaacgtg acgaaggaca ccattccgtc ctggttgccc tggccttggc cgggaacgga
      481 agcgctgatg tcggcggggc cgccgaacat ctcctcgccg gttgtgatgt ccagcgcatg
      541 gaggcggaag aaatacaggc cgttctcgat gttattggcg accacgtaga tggtcccgga
      601 atcgaggtcg attacgggag tacttaagat gcccacctcg ggcacgatgt cttcaagctc
      661 gtagtcggag ctgggcactg gtgggccaag ggtgactgtc cacagcggag cctgtggcgc
      721 gtcggcgtcg aacgcgtata cggtgttgtg catcgtggcc acaaagacca cgttatgcac
      781 tcccttgccg gggatagtca gatttctgac atagagcggc tgggcgtaaa taaatccgtc
      841 aaccggaagg gaaaagacct tcccgaagcg cgcgacattc acattggaaa cgttgagaat
      901 cgtttcgtgc aaattcgcat tggtcctgtc cttgtcgtag ttggcggtaa ctacatcgac
      961 ttgtgcggcg gccggagcaa gagtgaccag tggtagaaga agaaaacgga accagcccaa
     1021 ctttacgtcc tcccctcata agaacagtat acttctaccg gttttccgaa gtggggtact
     1081 tagataaagc ggtccggctc gatttgttgg gccatctgca aggcgcctcg ttgcggagtt
     1141 caatctgggt actcagggat ggccgatggc aggtgctctt tcatcaaggc acccggatgt
     1201 cgagtaccta acccaacctt tggatctgtg ctgggacggt ggtcgctgac atggcgatgg
     1261 atcactcaaa agacgccttc agtggcgcgt aacagtgtcc ggtgtgccgg cgactcgacc
     1321 gggatgcacc tggacaacat agtggcgctt ccatggatag tgctgtctag ccagccgaaa
     1381 caggcaacag accgctgtcg gaacggcgga acagcagagc cgccgtttac tacaggcgtc
     1441 cgctagaatg gccacatgcc tctgggagag agaatcccac ttgacgttcg gctgctctgt
     1501 tccattctgg ctatcacaat atgctggggc cttgctggcc ttgttttcag cctggtagtc
     1561 aaaggcccga acgcgctggc ggcgtggctg atctggggtg gcatcgtctg cttcgtaggg
     1621 tgggtacttg ttggtatccc actcgtggca gcaggcgacc ggatctgccg gattcgaccg
     1681 gcagtgttgg tgataggggt tggattctgc ggtgctctca ttatggcttt accctacttg
     1741 gctgccgtgc tcactcacaa tttcggagcg ggaaatcaca tggtagtcgc ggcagatgcc
     1801 acctttttga agttcgaggg gagtgccttc ctgattgccg ccacggcggc cttactttac
     1861 cgtggattgc tgatcaggtc ccaaaccatc gcgtcaagac cctgaagatc agcctaacgg
     1921 cgcggcctag actcactcac accgagcgcc ttctctccat ttcagggaga gcccatgtag
     1981 gtggacgtgg gcgtcccccc tcgatgcgtc acaccgacca gtccgctttt gtgcccgcac
     2041 gcgacgccgc cgaagctcat tcagctaatc tggtcttagt ggagcggttc cgcagagccg
     2101 cgctgagccc ccggcccgac aggcaagtca gccaccccga agacagtcgg cactgttcgg
     2161 tgcgcttttc tatttcaccg aaatcgtcgc tccgatggtt cctatggcga ccgattgggc
     2221 gccgccaggt agcgccatca gggaccaggg aaagaactgg tacaaacccg gcgcgacata
     2281 agtgggaacc tgcacgttaa tctgcaccac gccgcacggc agcgttggtg cgctaccggc
     2341 atataatgct ggagcgtact ggaccggaac tcccggaggt gaaccgtcgg caatgtccac
     2401 gcttatgccg gctgccaggc tcacggggcc gggcgggttg agcccgcccg tggcgcaggg
     2461 gggatcgatc aggccgaaac cggtgcccca aacggagacc acggaacccc tggccgccgg
     2521 gttcgcggga ccgttgatcg tgccatcctc gttcactgcc gccgcctgcg atgagacgcc
     2581 agggtggagc cggaagatgc ccggagatcc atagctcact accgacgcct cgatcgaccc
     2641 gactgcggcc cggttgtata ggacggttat gttcgtcttt gtctgaccgc tcagttccac
     2701 gggcgccagc gcattgattt gccgcgattg ggcgtacagc accggtgcgg gctggccgtc
     2761 aaagagaacc tgaacgcctg caagctcgcg tgtaacctgg ccctgatccg caacaccaat
     2821 atccgggccg attccgaatc cagtcagggt gatgaattca cctggcacca acgcgggtcc
     2881 cgtcgcggag tacggaatgg tcgacagagt cgcagcattc agaacggccg gcgacaggca
     2941 ggctggcgcc ttccatccgg tgccaccgaa ccggagtcgg gacttcaccc tgttgcccgc
     3001 gctatccatt gccagcagaa cagacccgtc tccggccaat gacagaccca cagtgaaggt
     3061 aacgccctga ctgacatagg tcgcgtcgag gagagcgcca tgaggatcga gatgggcaac
     3121 aaaagcatcg gttgccccga tcccgttgta gaattgggca ctgtgaaggc cgtcaaaaca
     3181 gacctgtggc gcggaagcgg tcaccgggaa acctgggccg gtgagcccgg cgatataagc
     3241 ctcgcccgag gcgctgaccg ccagttgagc gacgcccaga gacggtcgag tgacgccctg
     3301 ataaagggga tgatccgcac tcatcacata gctggaccac gacagagcgc tgccgtccgc
     3361 actgagcttg gcggcaaatc cggcgggcgc tgagttgttc cacatgggca ctattggatc
     3421 cggctcgaag ctgcccgaag tagtagggaa atcgagcgaa cttgtctggc cggcgacata
     3481 gatgttgcta gcggaatccg tttgtaccgc ggtaggtgtg tccgtgccgc ttccgccaag
     3541 ataggtggag aacactacgc cgccgctcgg gtcgagtctg gcgatgaaac cgttctgcgc
     3601 tcccatgagt cggctttgga ccgtcccggg cgtgactgga aagtccggcg aagtagtctg
     3661 accggcgacg atgacattgc ccgcggaatc cgccgagacc gcgttaacgg aatcctgagc
     3721 gctcccggcc agggagacgt gatagaggaa agcggagcca tcggcgctga gcttcaccac
     3781 gaaggcgtgt ccggtactgg attttccggc gatgtaggca ttaccttgtg cgtcaatggc
     3841 aatggccgac ggcatcccgg cggtatcggg caagtaggtg gaatacagta cgcggctccc
     3901 atcggcgctg atcttggctg caaacgactt ggctgtcgtg ctggcggcga tggcggcttt
     3961 ggcggtagtg ggaaaagaac cttccgtcga gcctgtgaca aaaacgttgc cggcgccatc
     4021 cacggccaga gcagtcgatt ggtccgcggt tgggcctccc aggtaggtgc cgaaaaccag
     4081 gttgccggat gcgtcgagct tgcccgcgta accgtcgggg cagggtccgg gaaatgagaa
     4141 aaagccattg ctgcttaaac aggtgccgcc gccgttctgg gtctgcgctg cgcctggggt
     4201 gaccggccct gccaggaaga aatacagatt cccggcgggg tcgaatactg ctggaccggc
     4261 caccggcacg gtcgtagcgg tagtggtaat gctggcgggt tgcgcccaca gaccgcccgc
     4321 gacgagggtc atcgccagtg cgttcgatgc cagaagggac ctcagtatgc tagccgccat
     4381 atttgcttga tgcggattgt tgcatccacg tcgcgactga tcccgcgttg tcgccaggtg
     4441 tttacagaag tttccggtgg ctctcgcgct cttggtcccc tgttcgcgcg cagtttccga
     4501 accgcgcaga gctcccgctg gcacaggctt agcctgtgtg gcaacacccc cgcggcggca
     4561 cagcctctac ggcttggcgc cgccgagtgc ggccgtcgcc agcgtacctc ccaagcccat
     4621 ggtctccacc gctgaggagg ggacgatgac catcgagccc ttctctttga tggcttcgta
     4681 cagcatgttc atcgcgcgca gatgcagcgc gaccggattg tcgcggtacg tgtccgcggc
     4741 ctgcgcgaat ctgtcggcga tctcggtttc ggccaggctg ataatcgtgc gcgcgcggcg
     4801 ctcgcgctcc gcctgcgctt cccgcgacat ggcgtcttga agttgcgacg gaatctgtac
     4861 gtcgcgaatc tcgaccgatt gcacggtaat cccccacggg ttcgtctttt cgtcgaggat
     4921 gcgctgcagt tcctggccga gcatctcgcg ttccgccacc atctggtgta gctcgtggcg
     4981 gccgatcgat tcccgcaacg cggtctgcgc gctaagcgag atggcctgct cgaagtcctg
     5041 cacttcgaga atcgattttt cggcgttcca gaccagccaa aacacgatgg cgtctacatt
     5101 cactggaacc gtgtctcgcg ttagcgttga ttcggccgag acgctgacca ctcgcacacg
     5161 ctggtccacc gtgcggctga ccgtatcgac aatcggaatg atgtgaaaca agccgggccc
     5221 gcgcaggccg atgtaacggc caaaccgcag cactgcgaac ttctcccatt ggtcggccac
     5281 gcggatcgag aagaggaggt agaggccgat ggccgcacag gggatcagta tcgggtgcct
     5341 tccggtggcg gcgtctgccg cgccgcccag cgcgatcgag atcacgaaca gtgccactcc
     5401 cacattactg actccaagct ccctgagacc tgtcttcatg gcgctacctt cctttcctga
     5461 tcagcctgca aatcacgcaa ttgctccaca agatcctgga tcgtgaagcc gccggcgccc
     5521 gcacgggcca cgaattcgcc aacgagctgg ttcaaccggc gctgccgctc gacctgatcg
     5581 cccttcacct tttgccggct gataaatgtg ccggttccct gttgtgtctc gagcaaaccg
     5641 cggatttcca gctctcgata cgctctcacc accgtgttcg ggttgatggc gaggtccacc
     5701 gcgacttggc gcacggtggg aagctggtcg cccggcttta atgcgccggc ggccatcccg
     5761 ccggtcacct ggtcgatgat ctggcggtag accggcacac cgctcttaag gtccagccgg
     5821 aagccgaagg gccgtttgct atcttggacc gtgctcgcca tctaaactaa tagtatacta
     5881 gttcaatagt acaagtcaag tactttctaa aagggcaaac gcgtgacaaa cagtgaagca
     5941 cggaatgacg ttcttgaacc gcgcgcgtaa catcctgcgg acttctcaaa cccacgttgc
     6001 caggggggta tcagcctcgg cctgcccctg ttaaggtgag gtgtctgggt ctcgtcgggg
     6061 tccggaggct ggcgggaacc gctagccgtg aacgtgtttc ttcgcttctt ccgattcgta
     6121 ctcgaccttt ttgccgctgc gggcttcgat gatggtgatc atagtcttcg cgcggccttt
     6181 cgcgatctcg atcacgacgc cttgcggctt gccttgggag tcgttgtagc cgatcgtcag
     6241 gaagtgcttg cgcttctttg agaacagcgc ccacacggtg atcacgatcg ccacgccgac
     6301 acggcggccc gccttttggc cgtattcgag agacgtgatg ctgccgtacg gaatttcgaa
     6361 tccgcctttg tcggacgtga agatgagttt ttcctcgttt tgtgtgtcca gcctgccttc
     6421 cgtcgcttca gccaggttct ttactgtgcc gccgatgtac ataacctcat caccccggac
     6481 agccgcgacg atcagctcgg caaataatac gaaagcaacg cacaatgcaa cggtttggcg
     6541 gacctgcatg ttcgctacct ccctgcgttc ttctttgcct cttctgattc gaactccact
     6601 tgtttgccag agcgggtctc gagcgtggtg agcaggcttc ggacgttgcc cttggagact
     6661 tcgagcacgg ccccctgatt cttcccggct tcgtcggcga agccaatggt cacgtagtgc
     6721 tttcgcttct tcgagaagag ggccagcggg ctgaccgcga gcgcaacgcc gactcgccgg
     6781 ccggccttct gcccgtattc gatcgaggag atctttgtgt acgtgatcga gagatcaccc
     6841 ttcttcgatg tgaacttcaa tgctgcggaa tcgtcgagta agagccggcc ttccgttttc
     6901 tcgggaatcg aggttatggt tccgcccaca tacatcgctt catcgccgcg gactgctgcg
     6961 agtgccaatg taggcaagag gattgttgcg cctccggatg gattgaggct ccttcatgga
     7021 agcgattctg gctgaaatgc tctgtcgcgt caagggtttt cgcatcgcgc agtccgaatt
     7081 tcaagttagc cccgtgacgc atgtcgcccg acttggtaag ccggcagtcc tgctggcagt
     7141 tgtgtaggtt ggctccccag agccgctccg agccccccgg cccggctatc tcccccgtga
     7201 aaccgggtcg cccgtggcat aggcttcaga cctggatgaa ccccatttca tgtcacctac
     7261 gttctcaccg cgcgaaaata ctgcgtcgcc cgtgggcgaa acacaaaata tgcgaacacc
     7321 gcacagagca gactgtgcat caccaactca aaccgcgagt ggaagccgct caggatgacg
     7381 tggtatgcga tccaggcaag cgcgagccag cgcgcccagt tgctgccacg gagcatatac
     7441 actccgcaga caatcgctat gagacgaacg acagaaaccc atacgagctc gtatggaaac
     7501 ggatgttgcg tcttgaactc tgtgaggtga taagccagcc caagtgcgcc cgccgctacg
     7561 tacagaaaac cgaagacggt caccgagagt ggacgctggt tcattcgagg atctttccga
     7621 cgagccacat gtgcccgaaa aggtcgatca gggcgccttt cagcatccgc ctgatcggga
     7681 cttcctcaca agagcgtaca cgcctcatcg aattccagac gcggcgctcg cgggtacagc
     7741 ctggaggcgt cgccgtagcc caggttgcac aggaagttcg actttacgtg gcctgcgggg
     7801 aagaactcct gctcacaccc ttcgcattcc ttgcccccgc cgaagaattc ttcgtcaagc
     7861 ctcgcgttat cgaagcccga catcgggcca cagtcgagcc cgagcgcgcg cgccgcgatc
     7921 atcaggtagg ccccctggag cgaggagttg cgcttggccg tgacctcgac cagttgtgga
     7981 ttccccgcga acagggcgca catcgcgggg ttgtggggga aaagcttcga cagcttctca
     8041 taaaagagca ggtcatacgc gatgatgacg gtaaccggcg ccgccatcgt tttggcgacg
     8101 ttcccggggg cgagcgccgg gcgtagtctc tctttcgcct cccgcgaacg gagaaagacg
     8161 aaccgggccg ggcttgcatt cgcgctcgtg ggtccccact tcatcaagcc atacagttgc
     8221 cggagcatat cgtcggtcac aggcttatcg agccaggcgc tgtgcgttcg cgcctcgcga
     8281 aatagttgat cgagagcttc gtctgccagc acatttttca tcggtctttg tttctccttt
     8341 gatgaggaaa ccatagaacg cgttgccttg tccgcgtagg tccttaggac cgcgcgccga
     8401 ggacgtgaag tgccagcctg ctaagcaccc gccgggtgcg cccgtcgaga aacgcccagg
     8461 tcagcgcctt gagcatgagc ggcggttgat acgccgggtt gtgcgccttt cggcgctcga
     8521 tcatgccggc gagcgtgctg tgcgggtcct cgcggaaagc gtcgagcatt gcggtctggt
     8581 gcacgcagga aatagtgcag aagggagcgc acggcttcgg gcgtgcggcc tcgcgtttca
     8641 ggtgatcgtg ggtg
//
LOCUS       DATIFQ010000403        20373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1093051, whole genome shotgun sequence.
ACCESSION   DATIFQ010000403 DATIFQ010000000
VERSION     DATIFQ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 20373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20373
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093051"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VLE22_19725"
     CDS             complement(<1..197)
                     /locus_tag="VLE22_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16691.1"
                     /translation="MMRMVGGLAALLIAGPLAGQSGPPVWKWELPQIQKVVQKVRAGR
                     DLTPKKWPGGARVAAALSFDM"
     gene            complement(353..2194)
                     /locus_tag="VLE22_19730"
     CDS             complement(353..2194)
                     /locus_tag="VLE22_19730"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HSB16692.1"
                     /translation="MRAFCVLAVLIVTPSVFAQESPIRLWAVSDGVRVSPITGNLIES
                     KPFVHSDYPELNPRRGNLVWDAANKTVSLKAARNEFTAFQLIIEAWEPQNQIDVKFAS
                     LVHEGGSARIEGRHIAFFKEWYTQVRRPTTGYERSSLGPDWYPDALMPYRGASRYTGF
                     PFSIPDIYNNIPSQKNHAVWIDVFVPFERAVAPPGRYTGTVEVTWQGGRDSVRVALDV
                     WDFALPQENHIQGDIWNGSMRNMPPEEELRYYQLAKQHRFLPLIYAYSPELLVNGTSV
                     KLDWTEYDRRLARYLDGSAFTEKHGYWGPGYGIPVDHMMLPFNIERERSSTGAWPMAL
                     PKAGRTAEYVAVWKEVGRQIRAHLDQDPNWRKVTKIAFLNGLDESYNEEAYEKMLFYG
                     QLLHDAMGRGWFRYRIDGGYSRQVMERLSKEVELWVCHTADFDIDDVKHFRPRGVDTW
                     FYGPMIYEQEKNSGCGSNTFLDLDLLINRGIGWVGWKYQAGWVEWEFDWNAYAAWYEA
                     ENYKGTSSVYNGSGQSIYRGLVMGYREPIPSIRLKAQRRGLQDYEYFWLLSKKAGVEA
                     SDAIVNRIVYKNPFGLKALLDIEIWKNNPDEWEKARIEAGKLIAGKP"
     gene            complement(2550..2627)
                     /locus_tag="VLE22_19735"
     tRNA            complement(2550..2627)
                     /locus_tag="VLE22_19735"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2590..2592),aa:Pro,seq:ggg)
     gene            complement(2665..3069)
                     /gene="atpC"
                     /locus_tag="VLE22_19740"
     CDS             complement(2665..3069)
                     /gene="atpC"
                     /locus_tag="VLE22_19740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HSB16693.1"
                     /translation="MAETFELEVATPEKLLVREHVTEAQIPAANGYVGILPGHSPLLA
                     ELGTGDLTYVSEGRRRHLAVSGGWIEVVGDRVRVVADIGEKADAIDLKRAEEALSRAR
                     DRLTHPPAGLDVARALNSLKRAQARIDAATHK"
     gene            complement(3077..4537)
                     /gene="atpD"
                     /locus_tag="VLE22_19745"
     CDS             complement(3077..4537)
                     /gene="atpD"
                     /locus_tag="VLE22_19745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSB16694.1"
                     /translation="MPTETSKNVVGKVIQVAGPAVDIQFPEGQIPIIYTAIRITSEGF
                     NVPEPIDIIVEVAQHIGEGRVRTIALQPTDGLVRGMMAESLDRPVTVPVGKETLGRVL
                     NVIGEPVDHMGPVNASKTYPIHRPAPTFEEQSTESQMFETGIKVVDLLEPYLKGGKIG
                     LFGGAGVGKTVIIQELIHNIALHHGGVSVFAGVGERTREGNDLWLEMQESGVIDPKDW
                     RKSKCALIYGQMTEPPGARLRVGLTGLTVAEYFRDEEGQDVLLFIDNIFRFTQAGSEV
                     SALLGRMPSAVGYQPNLATEMGELQERITSTRKGSITSVQAIYVPADDYTDPAPATTF
                     AHLDATTNLSRAIVELGIYPAVDPLASTSRILDPRIVGDEHYNTAQQVKQILQRYKDL
                     QDIIAILGIDELSDEDKLTVARARKIQKFLSQPFHVAEAFTGRSGKYVKVADTIRGFK
                     EIVEGKHDDIPEQAFYMQGTIEDALETAEQMKKVAA"
     gene            complement(4538..5398)
                     /gene="atpG"
                     /locus_tag="VLE22_19750"
     CDS             complement(4538..5398)
                     /gene="atpG"
                     /locus_tag="VLE22_19750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HSB16695.1"
                     /translation="MPSLIDIRRRIRSVKSTQQITRAMKLVSAAKLRRAQDRVIAARP
                     YASMLTRVLADVSEAARTNEQLATHPLLARRDERRIQLILITSDRGMAGAFNSNLIKA
                     AQRFLEEHAGAEVGIETVGRKGRDFFRKRGANLTGEMVGMTDKVGYADASELAKRASA
                     RFIAGEIDSVYLLYNEFKSILTQRLTVERILPTSLPEEAHGIDYIYEQPPEKLLEGLL
                     PRYVEVGIYRALLESAAAQQAARMTAMDAATSNAADMIDRLTLYMNRVRQASITKEII
                     EVVSGAAGVE"
     gene            complement(5415..6938)
                     /gene="atpA"
                     /locus_tag="VLE22_19755"
     CDS             complement(5415..6938)
                     /gene="atpA"
                     /locus_tag="VLE22_19755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSB16696.1"
                     /translation="MAQIRADEIARVLRDEIENYEKTVSVVETGFVISVGDGIARIHG
                     LELVMAGELIEFPHGVSGIALNLEEDQVGAVLLGDYAEIKEGDEVRRTGRIMSVPVGD
                     NLIGRVVNALGEPIDGKDPIATPHFSTIERLAPGVVERQPVKEPLQTGIKAIDAMIPI
                     GRGQRELIIADRQTGKTAIILDTIINQKGGDVTCIYVAIGQKRSTVAQVVKILEDAGA
                     MEYTIVVAATASDPAPMQYLAPYSGCAMGEHFRDNGRHVVCFYDDLSKHAAAYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSDKNGGGSLTALPVIETQAGDLSAYIPTN
                     VISITDGQIFLEADMFNANIRPAINVGLSVSRVGGNAQVRAMRQVAGTLRLDLAQYRE
                     LAAFAQFGSDLDKASLAQLNRGRRLTEVLKQGQYKPLPVEKQILIIFAGTNAYLDDLP
                     VEDVRRFEEELYRFADNAHPGVLKQIREKKAIDDALREEMHALLKEFKERFAAEQRD"
     gene            complement(6981..7529)
                     /gene="atpH"
                     /locus_tag="VLE22_19760"
     CDS             complement(6981..7529)
                     /gene="atpH"
                     /locus_tag="VLE22_19760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HSB16697.1"
                     /translation="MALAVASRYARALADLTTHPTRGVDSQAAMAELRAFENALKEFS
                     DLRNVILSPAVASARKRAVIGRLSGPLGISALIRNFLYVVTDHRRTRILPDIREAFQV
                     LVNERLGVVEARIAAARELTAEQRSAVAAELSRMTGKKVRCGFEVNESLIGGLTAQIG
                     STVYDGSVRGQLDGLRRKLAAP"
     gene            complement(7536..8174)
                     /locus_tag="VLE22_19765"
     CDS             complement(7536..8174)
                     /locus_tag="VLE22_19765"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HSB16698.1"
                     /translation="MKKSVIAFTIGLLLLVAAPSVFAQHASQTHGSSAAKSHGEGSRQ
                     HGDSGIWWKWANFAILAGGLGYLIAKKAPAFFESRTHAIQKDILEARAMREQAEAKAA
                     EIQRRLDNLSSEVDGLRHAGREELAAEEARLRAETAESIRKIKVRAEQELAAAVKTAS
                     QELKSHAADLAIELARQKIRSRMTAQIDDSLVRSFVTDLELRGRGQTEGEVN"
     gene            complement(8171..8620)
                     /locus_tag="VLE22_19770"
     CDS             complement(8171..8620)
                     /locus_tag="VLE22_19770"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HSB16699.1"
                     /translation="MQETLHALGGLLLKALPTFLLVILLHFYLKRVFFRPLDKVLRER
                     EEATEGARKTAEASLAAASKKAAEYEQAIRAARGEIYREQEETRRKWRQEQASTLEEA
                     RLETGDMIRQAKSQLAAEAAEARQTLAAESDRLAGEIVESILKGRAN"
     gene            complement(8770..9546)
                     /locus_tag="VLE22_19775"
     CDS             complement(8770..9546)
                     /locus_tag="VLE22_19775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HSB16700.1"
                     /translation="MSVPLEGKHALVTGGSKGVGKGIALELAAAGCNVAVNYNSDAGG
                     AAQTAAEISALGREAIAVRANVGAAGDVGAMFDEVLRRFPRLDILVNNAGVQTWKALL
                     DLEEREWDRVINTNLKGCFLCTQRAARHMKDHGGGRIINIGSGCNKIAFPKLVDYTAS
                     KGGIEMFTKVAAVELGPFGITVNCVAPGAIEIERTRQESGDYAGTWSKCTPLGRIGTP
                     ADIGRTVAFLAGPGADFITGQTVWVDGGLFTRPNWPEEYR"
     gene            9647..11593
                     /gene="speA"
                     /locus_tag="VLE22_19780"
     CDS             9647..11593
                     /gene="speA"
                     /locus_tag="VLE22_19780"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HSB16701.1"
                     /translation="MSLNISDGVVVREPTERWTVTDAGELYDVPRWGKGYFSVGPAGH
                     LLVHPSKDPKRSIDLKELVDTLVLRGISLPILIRFAEILKHRLEEIYQAFQNAIAEHG
                     YQGGYSCVYPIKVNQQKQVVEEVFQYGRPYKFGLEAGSKPELLAVLAIADNETPIICN
                     GFKDAEYIEMVMLAKKVGRQIIPVIEKYTELDLVVRYAQRVGVRPVIGVRIKLASRGS
                     GRWKSSAGYRSKFGLTVTEALRALNQLKADGMEDCLELLHFHLGSQITNIRQIKAAVI
                     ESARVYVEMRRAGAGLRYLDVGGGLGIDYDGSQTDFESSVNYTLQEYANDIVYHVQNV
                     CDEAEIPHPNIISESGRAVAAYHSALVFNVLGVSGFGEEELPKDLPEDSEQPLFDLQE
                     TYRALSSKNLLESYHDAQQALDAALNLFSLGYLSLVQRSLAENYYWTICRKIQRMAKE
                     LGDVAEELEPLDAMLSDTYFCNFSLFQSMPDSWAVKQLFPIMPIHRLEEQPARHGVLG
                     DISCDSDGKIDQFIDRRDVKRTLPLHPFNGQPYYLGAFLVGAYQEILGDLHNLFGDTN
                     AVHVSLGENDEIILDTVIKGDTVREVLDYVQFRSESLLARFRRDVEVAVREGRIGYEE
                     SGRLLRFYEEGLQGYTYLEDTHER"
     gene            11709..13553
                     /locus_tag="VLE22_19785"
     CDS             11709..13553
                     /locus_tag="VLE22_19785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HSB16702.1"
                     /translation="MSTTEVRPDESQTEGAGLSVVNDFSIQVATVNGSGSQTANTVLM
                     RSLFQMGIPVSGKNLFPSNIAGLPTWFTIRTSKRGWIGRKKEIDFLVAMNPETAREDV
                     LGLDAGAAVVYDEPLKLDSLRSDVHFYPVPFDKLVAPVCPEARLRKLVRNMIYDGVVA
                     QLLGIDLEEVRKALVKQFGARKAKAAELNWNAVLAGRDFAEQNLAKKDPFRVERMNAT
                     AGKIIIDGNAAAALGCVFAGATVVIWYPITPSSSMIEAMIGYLKRFRIDKETGKATFA
                     VVQAEDELAAIGMVIGAGWSGARSVTATSGPGISLMAEFTGLGYYAEVPAVIFDVQRV
                     GPSTGLPTRTMQGDLLSTAVLSHGDTQHPMLLPSSPEECFTMAGTAFNLAEYFQTPVF
                     VMTDLDLGMNNWMSDAFPYPTEPIRRGKVLTAEDLERLGGFARYKDVDGDGVGCRTLP
                     GTNHPTAAYFTRGSGHNEKAQYSERPDDYIQNVDRLALKFQSMRKHVPAPAISYNDKA
                     SIGVICYGTSRYAVEESCDQLTHEYGLETSHLRLKAYPFTDELREFVGRHSRVYVVEQ
                     NRDGQMLALMKLDLDPEYVQRLRSVRYYGGLPLDARTVTDEIVRQEGL"
     gene            13550..14593
                     /locus_tag="VLE22_19790"
     CDS             13550..14593
                     /locus_tag="VLE22_19790"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HSB16703.1"
                     /translation="MSTTVSPPPKKVNRIGLEVANYKGARTTLCAGCGHNAISERLIE
                     CFFEMGVQPWQVAKFSGIGCSSKSPAYFLNLAHGFNAVHGRAPAVGTGAVLGNHHIIG
                     ITVSGDGDTASIGIGQFMHMLRRNIPMIYIIENNGVYGLTKGQFSATADLGAKLKTGI
                     VNDLPPIDCCMLGIELGATLVARSFSGDKRQLSAILKASIAHKGLSVIDVISPCVTFN
                     DHEGSTKSYAYMKEHDEPLHEVDFVPFSEDISVEIEEGAVREVTLHDGSKLVLKKLEK
                     DYDPSDKLNAVRTIHEAEKSGHVLTGILYLNPSGKTLIDLLNMVDEPLATLPESRTRP
                     PKEALDQIMDELR"
     gene            14598..15347
                     /locus_tag="VLE22_19795"
     CDS             14598..15347
                     /locus_tag="VLE22_19795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HSB16704.1"
                     /translation="MNPEILRVVLVSPRNPLNIGAAARAMSNFGFSHLRLVNPYHVAF
                     QEAKSAVGAGHVLEAAYEFGSVAEAVADCSLVIGTTAAGPRELHHPIRRLEYGARLIR
                     KHSGTPPVAVLFGSEKHGLSNDDMSHCHWLMRVPTREGHRSMNLGQSVAVCLYELIRD
                     SRAALREPVRLRAASSMELERITELLIGMLGCSGYVNPRTRTSTENKVRRLVRRLGLS
                     SRDAEVWLGMLRQIRWKMNHNSGEDQHQERA"
     gene            15344..15559
                     /locus_tag="VLE22_19800"
     CDS             15344..15559
                     /locus_tag="VLE22_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16705.1"
                     /translation="MTKVELLFKLTRPLDESLMERIANAHSIYGMYRVQVLPNPDHLR
                     VEYDASRLSVHDVEAALHASGIPAERE"
     gene            complement(15569..16726)
                     /locus_tag="VLE22_19805"
     CDS             complement(15569..16726)
                     /locus_tag="VLE22_19805"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB16706.1"
                     /translation="MMRITAIDTYIAGNPWKNWLFAKVSTDEGIHGVGEGTLNYFGKT
                     VEAAIQELKPLILGMDPFQVEVISQRLIRDVYSEGAQIHMCAAAAIEIACWDIIGKAC
                     KQPVYNLWGGRCYDKLRAYANGWYRGPRTPESFAEKAKTVAGRGYTALKFDPFGSAWR
                     TLSRYDFDLTVDIIRAVRDAVGPSVDLLIEGHCRFNVATAVEFAEAIHQFRPAWFEEP
                     VPHTNIPAMVEVARRSPVPIATGESLSTKQQFAELLKYEVVNILQPEPLNLGGLYAAR
                     KIADMVDAHFGVIAPHSAQGPVCSAACVQLNASIPNFFIHEIFDEFNEPWEKEIVTNP
                     VEVIDGYIEIPERPGLGIDLNIEEILKHPYRQENYLPLFKPGWERREGASE"
     gene            complement(16745..17725)
                     /locus_tag="VLE22_19810"
     CDS             complement(16745..17725)
                     /locus_tag="VLE22_19810"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HSB16707.1"
                     /translation="MMRKILVFISLASSLGAQVKLTQGTDRIAVEIDGKPFTDLFIGS
                     AYSKPFLHPLRAASGTVVTRRFPMELVEGEARDHPHHRGLWFTHGDVNGIDFWVNEPG
                     DTRANVGRVVIRRVLDLKDGKKTGSLTASFDWQDPKGKSLLNELRTIVFYSHPRLRMM
                     DFDILLTAIEQVKFGDTKEGTFALRLASGLEEPRKDAPPVPPRTGKMVSADGKEGEQN
                     VWGTRSPWVDYFGEIDGEKLGVAILDHPTNPRHPTYWHSRSYGLFAANPFGLHDFLRD
                     KSQDGSLTLEPRQSVRFRYRVVIHPGDPASGAVAEMFKAYATMKLPTQKK"
     gene            17865..18389
                     /locus_tag="VLE22_19815"
     CDS             17865..18389
                     /locus_tag="VLE22_19815"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSB16708.1"
                     /translation="MGPLGWQETVFIFLLALLVFGPKKLPELGKTIGKAITEFRRASN
                     DLKATFDREMNSLEREGESLREITSSYHNEITSNYNYDSSYNYEDSYYGSSSNDSTAS
                     NSSTVSASATEGAESTTGVASDSGEATASATGEQETLQADAGGAEYGNPVEEPQPASS
                     AGDSSAPESKAVTT"
     gene            18407..19540
                     /gene="tatC"
                     /locus_tag="VLE22_19820"
     CDS             18407..19540
                     /gene="tatC"
                     /locus_tag="VLE22_19820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSB16709.1"
                     /translation="MGIDEERTGGSPPESQDSSEEGSVSPESDGRAGESGYGEESYVH
                     DDASPAVTKVEEKPAPAAESGGGGSGAPPKSGSDGSDDGNDDEEGMLRMSFMEHLEEL
                     RRRLLFSLAGVGVAFVLCLVFANQLWLIVSQPAVDALRRIGAEPNLAQITPMDAFTTI
                     WVKVPMLTAVFLASPWVLYQVWAFIAPGLYKRERRWAGPFVICTAGLFILGGLFAYFI
                     AFRFGLEFLLSIGRDINVKPVVSLTEYFDLFVNVTLGIGLVFELPVLLFFLTLLRIVN
                     PSFLLRNTRYAILLIVILAAVITPTPDVFNLMLFSVPMCLLYFVGIFASYLLVLHREN
                     RRFPWGVTLLVLFAILLVAAGAVYLAIAKYGFHLVPKWPFLIR"
     gene            19622..19858
                     /locus_tag="VLE22_19825"
     CDS             19622..19858
                     /locus_tag="VLE22_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16710.1"
                     /translation="MDLYKAIRELYEEKKRLEEAIASLEELLEAKAVTGNLNLDGLRK
                     RRGRKSMSAEERRIVSDRMKKYWAQRRAKGGKSA"
     gene            complement(19879..>20373)
                     /locus_tag="VLE22_19830"
     CDS             complement(19879..>20373)
                     /locus_tag="VLE22_19830"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB16711.1"
                     /translation="DPAQMRVLEIGCGAGRVTHALSEIFGEVHGVDVSSEMVELARQA
                     LKDRPKVHIHKNNGMDLSVLPDLPFDFAFSSIVFQHIPSREIIENYIREVHRLLRSGA
                     LFKFQVQGNIALKTSPEDTWLGVPISDRDAVEMALRCGFDPRYRHGAGEQYFWLWYFK
                     KETS"
BASE COUNT         3844 a         6304 c         6109 g         4116 t
ORIGIN      
        1 tccatgtcga atgagagtgc ggcggccaca cgggcgccgc cgggccattt cttgggcgtc
       61 aggtccctgc cagcacggac cttctgtacg accttctgta tctgagggag ttcccacttc
      121 caaacgggag gaccgctctg accggcgagc ggtccggcaa tgagcagcgc cgcgagacca
      181 ccaaccatgc gcatcatgac cgagaggata tcagaaggct ctgtgaggca agggcgcaaa
      241 gggaggcaca atcaatccat gaataggctc gaacggattt cccgtgtcgg gctggtggta
      301 gtcaccgaga ggcttggccg acgaaattcg acggcagccc ctgatgccgg gctcaaggtt
      361 tcccggctat cagcttgccc gcctcgatcc gggctttttc ccattcgtcc ggattgtttt
      421 tccagatttc aatgtcgagc agcgctttca gaccaaacgg gttcttgtac acgatccggt
      481 tcacgatcgc atcagacgct tcgacaccgg cctttttcga cagcagccag aaatactcat
      541 aatcctggag gcccctgcgc tgcgccttca agcggatgct gggaatcggc tcgcgataac
      601 ccatgactag accgcggtag atcgattggc ccgaaccgtt gtacacgctg ctggttccct
      661 tgtagttttc cgcctcatac cacgctgcat acgcattcca gtcaaactcc cattccaccc
      721 aaccagcctg gtatttccag ccgacccagc cgatcccccg attgatcaga agatcgagat
      781 ccagaaacgt gttcgaaccg cagccggaat tcttctcctg ctcatagatc atcggcccgt
      841 aaaaccaagt atctacacct cggggacgaa agtgcttcac atcgtcgatg tcgaagtcag
      901 ccgtgtgaca gacccacagt tccacctctt tgctcagccg ctccatgacc tggcgcgaat
      961 agccaccgtc gatgcgatat cggaaccatc cccgtcccat cgcgtcgtga agcagttgac
     1021 catagaagag catcttctcg taagcctctt cgttatacga ctcgtccagc ccgttcaaga
     1081 acgcgatctt ggtcaccttc cgccagtttg ggtcttgatc cagatgcgcg cggatctgcc
     1141 ggcccacctc cttccagaca gcgacgtatt ccgccgttct tcctgccttc ggaagggcca
     1201 tcggccatgc cccagtggaa cttctctccc gctcaatatt gaacggcaac atcatgtggt
     1261 ctacaggaat tccgtagccg ggtccccaat agccgtgttt ttccgtgaat gccgaaccgt
     1321 ccaggtacct ggcgaggcgc cggtcgtatt ctgtccaatc gagcttcacg ctggtgccgt
     1381 tcacgaggag ttccgggctg taggcgtaga tcagtggcag aaagcgatgc tgctttgcca
     1441 gttggtagta ccgaagctcc tcttctggcg gcatgtttct catggagcca ttccaaatgt
     1501 ccccttggat gtggttctct tgcggcagcg caaagtccca cacgtcaaga gcgacgcgga
     1561 cactgtctcg tccaccttgc caggtcacct caactgtgcc cgtgtaacgc ccgggaggtg
     1621 cgacagcgcg ttcaaaggga acgaacacat cgatccacac cgcgtggttc ttctgactgg
     1681 gaatgttgtt gtagatgtcc gggatcgaaa acgggaagcc ggtgtaccgg ctcgcccccc
     1741 ggtaaggcat cagtgcgtcc gggtaccagt cgggtccgag ggaggagcgc tcatatccgg
     1801 tagtcggtcg ccgcacctgg gtgtaccact ctttgaagaa tgcgatgtga cggccctcaa
     1861 tcctggccga gccaccctcg tggacgagcg aagcgaattt gacgtcgatc tggttctgcg
     1921 gctcccacgc ttcaatgatg agctgaaacg cagtgaactc atttcgcgcg gccttgagcg
     1981 acactgtctt gttcgcggcg tcccacacga ggttcccccg gcgtggattg agctccgggt
     2041 agtcgctgtg gacgaacggc ttactctcga tcagatttcc agtaatgggg ctgacccgca
     2101 cgccgtcgct taccgcccac aggcgaatgg gcgattcttg cgcgaaaacg ctgggggtaa
     2161 cgataagcac tgcgagaaca cagaatgcac gcatgaccga gtcacctctc tcccaacgcc
     2221 gggaaaggtc tccctgcgtc gtggatcgtt acagagtata tccgagcagt cagcaggtgc
     2281 ggcattactg gtaggccgga cgctgcataa gaagtctcat gttcacacgc agattcgttg
     2341 cggtgagttg cgtgatgaga tgatatgccc ggcgggacga ctggccctgg gggacgacga
     2401 gttaagaatg cacttcgagc ggcagggtgc tgcgctgatt tgtttgggat cagggcttgc
     2461 ccccgttttt ggcgtagagc caagcaagcg tcctttctgg actgctggcc tcgagccgtt
     2521 ataggctaaa gcattgaggt tattagattt ggtcgggccg ccgggatttg aacccgggac
     2581 ctcttgcacc ccaagcaagc gcgctagcca ggctgcgcca cggcccgatt ctttctcagt
     2641 gtaccgcaac ggctggttgg gccgtcactt gtgcgtggcg gcgtcgatgc gggcctgcgc
     2701 gcgcttcagc gagttcagcg cacgggctac atcaagaccg gccggcgggt gggtgagccg
     2761 gtcacgcgcg cgggagagcg cctcctcggc gcgcttcagg tcgatagcat ccgccttctc
     2821 gccgatatcg gccaccacgc ggacgcgatc gccgacgacc tcgatccagc ctccgcttac
     2881 cgccaggtgc cgccgccggc cctcggagac gtacgtcagg tcgcccgtgc cgagctccgc
     2941 cagcaggggg gagtgcccgg gcaggatgcc cacgtagccg ttcgccgccg gaatctgcgc
     3001 ttcagtcacg tgctcgcgca ccagcaactt ctccggcgtc gctacctcca actcgaacgt
     3061 ctccgccatg acaccctcag gccgccacct tcttcatctg ttcggcggtc tccaatgcgt
     3121 cttcgatcgt gccctgcatg tagaaagcct gttccgggat gtcgtcgtgc tttccttcga
     3181 cgatctcctt gaacccacgg atggtgtccg ctactttgac gtacttgccc gagcggcccg
     3241 tgaacgcctc agccacgtgg aaaggctgcg acaggaactt ctggatcttg cgcgcccgcg
     3301 ccaccgtcag cttgtcctcg tcggaaagct cgtcgatgcc aaggatcgcg atgatgtcct
     3361 ggaggtcctt gtaccgttgc agaatctgct tcacctgctg cgcggtgttg taatgctcgt
     3421 cgccgacgat gcgcgggtcg agaatgcgcg aggtcgatgc cagcggatcc accgcagggt
     3481 agatgccgag ttccacaatc gcgcgcgaga ggtttgtcgt ggcatcgagg tgcgcgaaag
     3541 tcgtcgccgg ggcaggatcg gtgtagtcgt cggcagggac gtaaatcgcc tgcaccgacg
     3601 tgatggatcc cttccgtgtc gacgtgatgc gctcctgtaa ctcgcccatc tcggtggcaa
     3661 ggttcggctg gtagcccacc gcggacggca tgcggccgag cagcgccgac acctccgagc
     3721 ccgcctgcgt gaagcggaag atgttgtcga tgaacagcag cacgtcctgg ccttcctcat
     3781 cgcggaagta ctccgcgacc gtcaggcccg tgagtcccac gcgaaggcgc gcaccgggcg
     3841 gctcggtcat ctggccatag atcaaggcac acttggattt ccgccagtcc ttcgggtcaa
     3901 taacgccaga ctcctgcatt tcgagccaga ggtcattgcc ctcgcgggtg cgctcgccaa
     3961 ccccggcaaa aaccgacacg cccccgtggt gcagggcaat gttgtgaatc agttcctgga
     4021 tgatcaccgt cttgccgacg cccgcaccac cgaacaggcc gatcttgcca cctttgaggt
     4081 acggttcaag caggtcgacg accttgatgc cggtctcgaa catctgcgac tcggtcgact
     4141 gttcctcgaa ggttggcgcc ggccggtgga tcgggtaggt cttgctcgcg ttcaccggtc
     4201 ccatgtggtc caccggttca ccgatcacgt tcagcacgcg tccgagcgtt tccttcccga
     4261 ccgggaccgt gacaggccgg tcgaggctct ccgccatcat cccgcgcact aagccgtcag
     4321 tcggctggag tgcaatcgtg cgcacccgac cctcgccgat gtgctgcgcc acctccacga
     4381 tgatgtcgat cggctcgggc acattgaaac cctcgctcgt gatgcggatg gccgtgtaga
     4441 tgatgggtat ctgcccttcc gggaactgga tatccacggc ggggccggct acctggatca
     4501 ctttgccgac cacgttcttc gatgtttccg ttggcatcta ttcaactcct gctgcaccgc
     4561 tgactacttc gatgatttcc ttcgtaatgc ttgcctggcg cacgcggttc atgtacaaag
     4621 tcagcctgtc gatcatgtcg gccgcgttcg atgtggcggc gtccatcgcc gtcatacgcg
     4681 ccgcctgctg cgccgcggcc gactcgagca gcgctcggta aatcccgact tccacgtatc
     4741 gcgggagcag tccctccagc agtttctccg gcggctgctc gtaaatgtag tcgatgccgt
     4801 gcgcctcttc aggcagcgac gtcggtagga tgcgctccac cgtcagccgc tgcgtgagga
     4861 tgctcttgaa ctcgttgtac agcaggtata cggagtcgat ctcaccggcg atgaaccggg
     4921 cgctggcgcg tttcgccagc tcggaggcgt cggcgtagcc gactttgtcc gtcatgccca
     4981 ccatttcgcc ggtcaagttg gcgccacgct tgcggaagaa atcgcgtcct ttacggccga
     5041 cggtttcgat ccctacctcg gccccggcat gctcctcgag gaatcgctgc gccgccttga
     5101 tgagattcga gttgaacgct ccagccatgc cacggtcgga cgtgatgaga atgagctgga
     5161 tccgccgctc gtcccgacgg gccagcagcg ggtgagtggc cagttgctcg ttcgtccggg
     5221 cggcctccga gacgtcggcc agcacccggg tcagcatgga ggcgtagggc cgcgccgcga
     5281 tcacccggtc ctgagcccgc cggagcttgg ccgcggaaac cagtttcatg gcgcgggtaa
     5341 tctgctgcgt gctcttgacg gagcggatgc ggcggcggat gtcgatcagg ctgggcatcg
     5401 gttacctcac cccgtcagtc gcgctgctcg gcggcgaaac gctccttgaa ctcctttagc
     5461 agcgcgtgca tctcctcgcg caacgcgtcg tcgatggcct tcttctcgcg aatctgcttc
     5521 agcacgccgg ggtgtgcgtt atcggcaaag cggtacaact cttcctcgaa tcgtcgaacg
     5581 tcttccactg ggaggtcgtc gaggtaggcg ttggtgccgg caaaaatgat cagaatctgt
     5641 ttttcgacag gcagcggctt gtattggccc tgcttgagca cctcggtcag ccggcgaccg
     5701 cggttcaatt gcgccaggga ggccttgtcc agatccgagc cgaactgggc gaaggcggcc
     5761 agttctcggt actgtgccag atcgaggcga agggttccgg ccacctgccg catcgcgcgc
     5821 acctgggcgt tgccgccgac gcggctcacc gagagaccga cgttgatggc gggccggatg
     5881 ttggcgttaa acatatccgc ttcgagaaag atctgcccgt cggtgatgga aatgacgttg
     5941 gtcggaatgt atgccgacag gtctccagcc tgtgtctcga tcaccggcag tgcagtgagc
     6001 gatccgccac cgttcttgtc cgacagcttc gccgcgcgct ccagcaaccg gctatggagg
     6061 tagaacacat cacccgggta ggcctcgcgc cccggcggcc tgcgcaagag cagtgagatc
     6121 tcgcggtagg cagccgcgtg tttcgacaga tcgtcgtaga aacagaccac gtgccggccg
     6181 ttatcgcgga agtgctcgcc catggcgcag cccgagtacg gggcgaggta ctgcatcggc
     6241 gcggggtcgg acgcggtggc ggccacaact atggtgtact ccatcgcgcc ggcatcctcg
     6301 aggatcttca cgacctgggc caccgtggat cgcttttgac caatggccac atagatgcaa
     6361 gtgacgtcgc cgcccttctg gttgatgatc gtgtcgagaa tgatagccgt tttgccggtc
     6421 tggcggtcgg cgatgatcaa ctcgcgctgg ccacggccaa tggggatcat ggcgtcgatt
     6481 gccttgatac cggtctgaag cggctccttc acaggctgcc gctcgacgac tcccggggcc
     6541 agccgctcga tcgtactgaa gtgcggcgtg gcgatcggat ccttaccgtc aatcggttcc
     6601 ccgagtgcat tcacgacccg gccgatcaag ttgtcgccga cggggaccga cataatgcgc
     6661 cctgtgcggc gcacttcgtc gccttccttg atctccgcgt agtcgccgag cagcactgcg
     6721 ccaacctggt cttcctcgag gttcagcgcg atgccggaca caccatgcgg gaactcgatc
     6781 agctcaccgg ccatcaccag ttccaggccg tggatgcggg cgatgccgtc tcccacggag
     6841 atcacgaaac cggtttccac gacgctgacc gtcttctcgt agttctcgat ctcgtcgcgc
     6901 agcactcgcg cgatttcatc cgctcgaatc tgagccataa ccttcggggg cagggcgttc
     6961 catccgcctc ccccaacctc ctatggagct gccaatttgc ggcgcaggcc gtcgagctgc
     7021 ccgcgcactg aaccgtcgta aaccgtcgag ccgatctgcg ccgtcaaccc gccgatcagc
     7081 gactcgttaa cctcgaagcc gcaccgaacc ttcttccccg tcatccgcga cagttccgcg
     7141 gcgaccgccg accgctgctc cgccgtcagc tccctggccg cagcgatccg ggcctcgact
     7201 acgccgagtc gttcgttgac cagaacctgg aaggcctcac gtatgtcggg caggattcgg
     7261 gtgcgccggt ggtccgtgac aacatacaaa aaattccgaa tgagcgctga aatccccagc
     7321 ggcccggaaa gccgcccgat gaccgcgcgc ttgcgggccg aagcgaccgc cggtgagagg
     7381 atcacgttgc gtaagtcgga aaattccttc agcgcgttct cgaacgcccg aagttcggcc
     7441 atggccgcct gcgagtccac gccccgagtg gggtgggtgg tgagatcggc cagcgcccgc
     7501 gcgtaccttg atgcaaccgc cagagccatg gttggctagt tcacctctcc ttcggtttga
     7561 ccgcgaccac gcagttcgag gtcggttacg aaggaccgaa ccagggagtc gtcgatctga
     7621 gccgtcatcc ggctccggat cttctggcgc gccaattcga tcgcgaggtc cgcggcgtgg
     7681 gacttcagct cctggctggc tgtcttgacc gccgcggcga gttcctgctc ggcccggacc
     7741 ttaatcttgc gaatggattc cgccgtctcc gcccgcaggc gagcctcttc ggctgccagt
     7801 tcctcgcgtc cggcatgccg cagaccgtca acttccgagg aaaggttgtc gaggcgccgt
     7861 tggatttccg ccgcctttgc ctcggcctgt tcgcgcatgg cgcgggcttc cagaatgtct
     7921 ttctgtatgg cgtgggtgcg cgactcgaag aacgccggcg ctttcttggc gatcaagtac
     7981 ccgagcccgc cggcgaggat ggcgaagttg gcccatttcc accagatacc gctgtcaccg
     8041 tgctgtctgg atccctcgcc gtgagactta gccgcgctcg agccgtgggt ctgggaagcg
     8101 tgctgggcga acaccgaggg tgcggcgacc agaaggagga ggccaatggt gaaggcgatg
     8161 acggactttt tcaattcgct ctccccttaa ggatcgactc gactatctca ccggccagcc
     8221 tgtcgctttc agccgccaag gtttgccgcg cttcggctgc ctcggcggca agctgactct
     8281 tggcctgccg gatcatgtcc cctgtttcga gccgtgcttc ctcaagggtg ctcgcctgct
     8341 cctggcgcca cttgcggcgt gtctcctctt gctcacgata gatctcgccg cgtgcagcgc
     8401 ggatggcctg ctcgtactcg gcggcttttt tcgacgctgc ggcaaggctc gcttcggccg
     8461 tctttcgggc gccttcagta gcctcttcgc gttcccgaag gactttgtcc agggggcgga
     8521 agaaaacccg cttcaggtaa aagtgcagca ggatgacgag gagaaaagtc ggcagtgcct
     8581 tcagtaaaag cccaccgagc gcgtgcagag tttcttgcat ctgagacttc ttgaattttc
     8641 agatcagaaa gggagcgcct gctgaagact actatggaca gaggctcaaa gcgcttacat
     8701 taacacaccg tccccagact cgcaaccgca cgccggggtg gtgcacccgg ctatcggatc
     8761 cctttttcgc taccggtact cctcgggcca gttcggccgg gtgaagagcc ctccatccac
     8821 ccaaacggtc tgcccagtga tgaagtccgc ccccggcccc gcgaggaaag ccactgttct
     8881 gccgatgtcc gcaggcgttc cgatccgccc aagcggtgtg catttcgacc aggtaccggc
     8941 gtagtctccg gactcctgtc tggtccgctc gatctcgatc gctccgggcg caacgcaatt
     9001 cacggtgatt ccgaacggtc ccaattcgac tgccgcgacc ttggtaaaca tctcaatccc
     9061 gcccttgctg gcggtgtagt cgacgagctt tggaaacgcg atcttgttac atcccgaacc
     9121 aatattaatg atccggccgc cgccatggtc cttcatgtgg cgggcggcgc gttgcgtgca
     9181 caggaagcag cctttcaggt ttgtattgat gacgcggtcc cactcccgtt cttcgagatc
     9241 gagcaacgcc ttccaggttt ggacccctgc gttattgacc agaatgtcaa gccgcgggaa
     9301 gcgccgcaac acctcgtcga acatcgcgcc gacatcgccc gcagcaccca cattagcgcg
     9361 cacggcaatg gcttcgcgtc cgagagcgga gatctccgca gccgtttgcg cggcgccgcc
     9421 ggcatcggag ttgtagttga ccgccacgtt acagccggcc gccgccaact ccagcgcaat
     9481 gcctttgccg actcctttgc tgccgccggt gaccagtgca tgcttccctt cgagtggcac
     9541 ggacatggca acgattctat cgaatcctgc cagttgcggc cgccgtcaaa caatctactt
     9601 gccgagtacg gcttttcgaa ggacacttta agtctatgga ggcgacttga gcctgaatat
     9661 ctccgacgga gtggtcgtcc gcgagccgac cgaacgatgg acagtcacgg atgccgggga
     9721 gctctatgac gtgcccaggt ggggcaaggg ttatttctcc gtgggtccgg ccggccatct
     9781 gctggtgcac ccgtcaaagg atccgaagcg ctccatcgac ctgaaggagc tcgtcgacac
     9841 ccttgtcctt cgaggaatct cgctgccgat cctcatccgg ttcgccgaga tcctgaagca
     9901 tcggctcgag gagatctacc aggcgtttca gaatgcgatc gccgagcacg gctaccaggg
     9961 cggttacagt tgcgtctacc cgatcaaagt caaccagcag aagcaggtgg ttgaagaagt
    10021 tttccagtat ggccggccat acaagtttgg actcgaagcc ggctcgaagc ccgaattgct
    10081 tgcggttctg gccatcgccg ataacgagac gccgatcatc tgtaacggct tcaaggacgc
    10141 cgagtacatc gagatggtca tgctggccaa gaaagtcggg cggcagatca tcccggtaat
    10201 cgagaagtac accgaactgg acttggtggt gcggtacgcg caacgggttg gcgttaggcc
    10261 ggtaatcggg gtgcgcatca agttggcgag ccgcggctcg ggacggtgga agtcttccgc
    10321 cgggtaccgg tccaagttcg ggctcaccgt aaccgaggcg ttgcgcgcgc tcaaccagtt
    10381 aaaggccgac ggcatggagg attgcctgga gttgttgcat ttccacctcg gcagccagat
    10441 caccaacatc cgccagatca aggcggcggt gatcgaatcc gcccgcgttt atgtcgaaat
    10501 gcggcgcgcg ggggcgggtc tgcgctatct ggacgtcggc ggcgggctag gtatcgacta
    10561 cgacggttcg cagacggact tcgagtccag tgtgaactac acgcttcagg aatacgcgaa
    10621 cgacatcgtc tatcacgttc agaacgtctg cgacgaagcg gagatccctc accccaacat
    10681 catctccgag agcggccggg cggtggctgc ttatcacagt gccctcgtgt tcaatgtgct
    10741 cggcgtgagc ggtttcgggg aggaggaact ccccaaggac ctgccggagg attccgagca
    10801 gccactgttc gacctccagg agacctaccg tgcgctcagc tcgaagaacc tgctcgagag
    10861 ctaccacgac gcacagcaag cgctggacgc ggcgctgaac ctgttcagcc tcgggtacct
    10921 ctcgcttgtc caacgctcgc tggctgaaaa ctactactgg acgatctgcc gcaagatcca
    10981 gcgcatggca aaggaactcg gtgatgttgc cgaggaactg gagccgctcg acgccatgct
    11041 ttccgacacg tacttctgca atttctcgct cttccagagc atgccggaca gttgggcggt
    11101 gaaacagctt tttccgatta tgccgatcca ccggctcgag gagcagcccg cgcgtcacgg
    11161 ggtgctggga gatatctcct gcgattcgga cggcaagatc gaccagttca tcgaccggcg
    11221 cgacgtcaag cgcacgctcc cgctgcaccc gttcaacggc cagccttatt acctcggagc
    11281 gttccttgtt ggcgcgtacc aggaaatcct cggtgacctc cataacctgt tcggggatac
    11341 caacgcggtc cacgtgagcc tcggcgaaaa cgacgaaatt atcctcgaca ctgtgatcaa
    11401 gggcgacacc gtgcgcgagg tcctcgacta cgtgcagttc cgctccgaaa gcctgctggc
    11461 acgattccgc cgtgatgtcg aggtggcggt gcgtgagggc cgcatcgggt acgaggaatc
    11521 cgggcgcctg ctgcgcttct atgaggaagg cctgcaaggc tacacgtacc tcgaagacac
    11581 ccacgagcga tagttttccg gcacaggttt ctcaaccaag tgcgttgatc cgcggcactg
    11641 caaacagttt gcatataatc gaataagggg aggggcgcct tcacaccccg catgaggaaa
    11701 acacatcaat gtccaccacg gaagtgaggc cggacgaatc ccaaacagag ggagccggcc
    11761 taagcgtagt caacgatttc agtattcagg ttgcgacggt caacggttcg gggagccaga
    11821 ccgccaacac tgttctgatg cggtcgctgt ttcagatggg aattcccgta tcgggcaaga
    11881 acctcttccc gtcgaatatc gccggcctgc cgacgtggtt caccatccga acaagcaagc
    11941 gcggctggat cggccgaaag aaagaaatcg actttctcgt cgccatgaac ccggagacgg
    12001 cgcgggagga cgtgctcgga ctcgatgcgg gcgcggcggt tgtatacgac gaaccgctca
    12061 aactcgacag tctgcgttcc gacgtccact tctacccggt gccgttcgac aaactggtgg
    12121 cgcccgtgtg tccggaagcc cggcttcgca agcttgtccg caacatgatc tatgacggcg
    12181 tggtcgctca gttgctcggc atcgatcttg aagaggtccg gaaggcgctg gtgaagcagt
    12241 tcggcgcccg gaaggccaag gcggccgaac tcaactggaa cgcggtgctt gccggacggg
    12301 actttgccga gcagaacctc gccaagaagg atccgttccg ggtcgagcgg atgaatgcca
    12361 cggccggcaa gatcatcatc gatggcaatg cggctgccgc cctcggctgt gtcttcgccg
    12421 gggccacggt agttatctgg tacccgatca ctccgtcgtc gtcgatgatc gaagcgatga
    12481 tcggttacct gaagcgtttc cggatcgaca aggaaaccgg gaaagcgacc ttcgccgttg
    12541 tgcaggccga ggacgagctg gccgcgatcg gcatggtaat tggcgcgggt tggtcgggcg
    12601 cccgctcggt gacggccacg tcgggtcccg gcatttccct gatggcggaa ttcacaggcc
    12661 tcggttacta cgccgaggtg ccagcggtaa tcttcgatgt gcagcgggtt gggccttcca
    12721 ctggcctgcc gacccgcacg atgcaagggg acctgctttc caccgcggtg ctttctcatg
    12781 gggacacgca gcatccgatg ttgctaccgt cgtcacccga ggagtgcttc accatggccg
    12841 gcacggcgtt caacctcgcg gagtacttcc agacaccggt gttcgtgatg accgacctcg
    12901 acctcggcat gaataactgg atgtccgatg cgttcccgta ccccaccgag ccgatccgcc
    12961 gcggcaaggt gctgacagcg gaagatttgg agcggctcgg tggtttcgcg cgttacaaag
    13021 acgtggacgg ggacggcgtg ggttgtcgca ccctgcccgg cacaaaccac cccacggccg
    13081 cctacttcac tcgcggcagc gggcacaacg agaaggcgca atacagcgag cggccagacg
    13141 actacatcca gaacgtggac cggcttgcgc tcaagttcca atcgatgcgt aagcatgtcc
    13201 cggccccggc gatctcttat aacgacaagg cgtctattgg tgtgatctgc tacggcacgt
    13261 cgaggtatgc ggtcgaagag agttgcgatc agctcacgca cgaatacggc ctcgaaacca
    13321 gccatctccg gttaaaggcg tatcccttca cggacgaact gcgggaattc gtcggccgtc
    13381 actctcgcgt ctatgtcgtc gagcagaacc gcgatgggca gatgctggcg ctgatgaagt
    13441 tggatctgga cccggagtac gttcagagac tgagaagcgt ccgctactac ggaggtttgc
    13501 cgctcgacgc tcgcaccgtt accgacgaaa tcgtccgaca ggagggacta tgagtacgac
    13561 tgtttcccca ccccccaaga aagtcaatcg gatcggcttg gaagtcgcca actacaaagg
    13621 agcacggacg accctttgcg ccggctgtgg acacaacgcc atctccgagc gactgatcga
    13681 gtgtttcttc gagatgggcg tccaaccgtg gcaagtggcg aagttttcag ggatcggatg
    13741 ctcatcgaag tcgccggcct attttctgaa cctcgcccat ggattcaatg cagtccacgg
    13801 acgcgctccg gcggtcggca ccggcgctgt gctgggcaac caccacatca tcggcatcac
    13861 cgtcagcggc gatggtgaca ccgcttcgat cgggatcggc cagttcatgc acatgttgcg
    13921 ccggaacatc ccgatgatct acatcatcga gaacaacggc gtctatggtc ttacaaaagg
    13981 ccagttttcc gccaccgcgg acttgggcgc gaagctgaag accggcattg tgaacgattt
    14041 gccgccaatc gactgctgca tgctcggtat cgaactcggc gcaacgttgg tggcgcgttc
    14101 gttttcaggc gacaagcggc agctctcagc gatcctgaag gcgtccatcg ctcacaaggg
    14161 gctgtcggtc atcgacgtca tttcaccgtg cgtcaccttc aacgaccacg aaggatcgac
    14221 gaagagctac gcctacatga aggagcacga cgaaccgctc catgaagtgg acttcgtccc
    14281 attctccgaa gacatctcag tggagatcga ggagggtgcg gtacgagaag tcacccttca
    14341 tgacggttcg aaacttgtgc tcaagaagct cgaaaaagac tacgacccga gtgacaagct
    14401 caacgccgtt cgcaccatcc acgaagccga gaagagcggc cacgtattga ccggtattct
    14461 ctacctgaat ccctctggga aaacgctgat cgaccttctg aacatggtcg acgagccact
    14521 ggcgacgctg cccgaaagca ggacgcggcc gccgaaggaa gcgctcgacc agatcatgga
    14581 cgagttacgc tgagggagtg aacccggaga ttctgcgcgt cgtcctggta tctcctcgca
    14641 atcccctcaa catcggggcg gccgctcgtg cgatgagcaa cttcggcttc tcgcatttgc
    14701 ggcttgtgaa tccatatcac gtagccttcc aggaggcgaa gtccgcggtc ggcgccgggc
    14761 acgtgctgga ggcagcctat gagtttggtt ccgtcgcgga agctgtagcc gactgttccc
    14821 tcgtcatcgg caccaccgcc gccggcccgc gcgaactcca ccaccccata cgcaggcttg
    14881 aatacggcgc tcgtctcatt cggaaacact ccggcacgcc cccagtggcg gttctgttcg
    14941 gctcggagaa gcatgggtta tcgaacgacg acatgagcca ctgccactgg cttatgcgcg
    15001 ttccgacgcg ggaaggacac cgctccatga atctcggcca gtcggtggcc gtgtgcctct
    15061 acgagctgat acgggactcg cgcgctgcct tgcgcgagcc ggtgcggttg cgtgcggcgt
    15121 cgtcgatgga actggagagg atcacggagt tgttgatcgg gatgctcgga tgcagcggat
    15181 acgtgaaccc acgcacgcgg acgtctaccg agaacaaagt gcggcgtctt gtccgaaggc
    15241 tcggcttatc ttcccgagat gccgaggtgt ggttggggat gctgcggcag atccggtgga
    15301 agatgaatca caatagcggg gaagaccaac accaggagcg ggcatgacca aagtggaact
    15361 cctcttcaag ctcacgcggc ctcttgacga atcgttgatg gaacggatcg cgaacgcgca
    15421 cagcatctac gggatgtatc gcgtccaggt gctcccaaat cccgatcacc tgcgggtgga
    15481 gtacgacgcc tcgcggctca gtgttcatga cgtggaggcc gcgctgcacg cttccggcat
    15541 tccggccgag cgcgagtagc ccgccttact actccgatgc gccctcgcgg cgctcccatc
    15601 ccggtttgaa tagcggcagg tagttctcct gccgatacgg gtgtttcagg atctcttcga
    15661 tgttcaggtc aattccgaga cccgggcgct ccgggatctc gatgtacccg tcgatcacct
    15721 cgaccgggtt cgtcacgatc tccttctccc acggttcgtt gaactcgtcg aaaatctcgt
    15781 ggatgaagaa gttcgggatc gaagcgttga gctggacgca agctgccgag cagacggggc
    15841 cctgggcgct gtgcggggcg atcacgccga aatgggcatc caccatgtcc gcgatcttgc
    15901 gcgcggcgta aagtcctccc aggttcagcg gctccggttg caggatgttc accacctcgt
    15961 acttgagcag ttcggcgaac tgctgcttcg tcgacaggct ctcaccggtc gcgatcggaa
    16021 ccggactgcg gcgggcgact tccaccatcg ccggaatgtt cgtgtgcgga accggctcct
    16081 cgaaccatgc cggccggaac tggtggatcg cctcggcaaa ctccactgcg gtcgccacgt
    16141 tgaaccggca gtggccctca atgagcaggt cgacggacgg gccgacagca tcgcgcaccg
    16201 cccggatgat gtccactgtc aggtcgaaat catagcgtga cagtgtccgc caggcgctgc
    16261 cgaacgggtc gaacttcagc gccgtgtagc cgcgtccggc cacggtcttt gccttttcgg
    16321 cgaagctctc cggcgttcgc ggaccgcggt accatccgtt cgcgtaggcg cgcagcttat
    16381 cgtaacagcg gccgccccac agattgtaga cgggctgttt gcaggccttg ccgatgatgt
    16441 cccagcaggc gatttcgatt gccgcggcgg cgcacatgtg gatctgcgcc ccctccgaat
    16501 acacgtcacg gattaaccgc tgggagatca cttccacctg aaacggatcc atgccaagga
    16561 tcagcggttt cagttcttgg atggccgcct caacggtctt gccgaaatag ttgagcgttc
    16621 cctcgccgac gccgtggatc ccttcgtcgg tcgagacttt ggcgaacaac cagttcttcc
    16681 agggattacc cgcgatgtac gtgtcgatag cggtgattct catcatatcg ttccgactct
    16741 cgacctactt tttctgtgtg ggtagcttca tggtcgcata tgccttgaac atctcggcga
    16801 ccgctccgct cgccggatct ccggggtgga tcactaccct gtaccggaag cggaccgact
    16861 gccggggttc gagcgtcagg ctgccatcct gggacttgtc gcgaagaaaa tcgtgcagcc
    16921 cgaaggggtt ggccgcaaac aggccgtagc tgcgtgaatg ccagtaggtc gggtgccggg
    16981 ggttggtcgg atgatcaaga atcgcgactc caagcttttc gccgtcaatc tcgccgaagt
    17041 agtcgaccca cggggaccgt gtgccccaga cgttctgctc gccttccttg ccatcggcgc
    17101 tgaccatctt gccagtcctg ggggggacgg ggggtgcgtc cttccgcggc tcctcaagac
    17161 ccgaagcgag acgcaaagcg aatgttccct cttttgtgtc cccgaacttc acctgctcga
    17221 tggccgtcag caggatgtcg aaatccatca tccggagccg cgggtgggag tagaagacga
    17281 tcgtgcgcag ttcgttcagc agcgatttcc ccttgggatc ctgccaatca aacgacgccg
    17341 tcagtgagcc cgtttttttg ccgtccttga gatcaagcac tctgcgaatc acgacgcggc
    17401 cgacattggc ccgcgtgtcg cccggttcat tgacccagaa gtcgatgccg ttcacatcgc
    17461 catgtgtaaa ccagaggccg cggtgatgcg gatggtcccg tgcctctccc tcaacaagtt
    17521 ccatcgggaa gcggcgggtc acgaccgtcc cgctggccgc ccgcaagggg tgcagaaacg
    17581 gctttgagta ggccgaaccg atgaacaaat ccgtgaaggg cttaccgtcg atttcgaccg
    17641 cgatccggtc agttccctgg gtgagtttga cctgtgcccc gagggatgaa gctaacgata
    17701 tgaagacaag gatcttgcgc atcatgtccg acagtacagc acagccggac ggggccgctc
    17761 aagtgacggc ccgcggctgt atccggtagc ccgccggcaa aatgcgtgcc ttcccgcttc
    17821 ggtgctatca tgagagcgag cgaatctcca agggaggaat acggatgggt ccgctgggct
    17881 ggcaggaaac ggtgttcatc tttctgctcg ccttgctcgt cttcggtccg aagaagttac
    17941 cggagttggg caagacgatc gggaaggcca tcaccgaatt tcgccgcgct tcgaacgatc
    18001 tgaaggcgac tttcgatcgg gaaatgaaca gcctggagcg ggaaggtgaa tcattgaggg
    18061 agatcaccag cagttaccac aacgaaatca cgagcaacta caactacgac tcttcctaca
    18121 attatgagga cagctactac ggctcgtcct cgaacgattc caccgcatca aactcgtcca
    18181 ctgtaagcgc atccgcaact gagggcgctg aatcgacgac tggagttgct tcggattccg
    18241 gcgaggccac ggcctccgcc acgggggagc aggagaccct gcaagcggat gccggcggcg
    18301 ccgaatacgg caacccggtg gaggagccac agccggcgtc ttcggccggt gattcctccg
    18361 cgcctgagtc caaggcagtt acgacgtagg gcaacctcga gaattcatgg gcatcgacga
    18421 agaacgcacg ggaggctcgc ctcctgaaag tcaggactcg tccgaggagg ggagcgtgtc
    18481 tcccgagtcg gatgggcgcg ccggcgagtc cggctacggc gaggagtctt acgtacatga
    18541 cgacgcctcg cccgccgtca cgaaggtcga ggagaaaccg gcgccagccg ccgaatccgg
    18601 gggcggtggg agcggcgcac cccccaagtc aggttccgac ggctccgacg atggcaacga
    18661 cgacgaagag ggcatgctcc ggatgtcctt catggagcat ctggaggaac tgcggcggcg
    18721 gcttcttttc agccttgccg gcgtcggcgt cgcgttcgta ctttgcctgg tcttcgccaa
    18781 ccagctttgg ctgatcgtca gccagccggc ggtggacgcc ctgcgccgga tcggggccga
    18841 accgaacctc gcgcaaatca caccgatgga cgcgtttacc actatctggg tgaaggtgcc
    18901 gatgctcacg gcagtgttcc tggcttcgcc atgggtgctc taccaggtct gggccttcat
    18961 cgcgccgggg ctctacaaac gcgagcgccg gtgggcaggg cccttcgtca tctgcacggc
    19021 ggggcttttc atcctcggtg gcttattcgc ctacttcatc gcctttcgat tcggactgga
    19081 gttcctgctc agcatcggcc gggacatcaa cgtgaagccg gtcgtatcgc tgaccgagta
    19141 cttcgatctg ttcgtcaacg tcacactcgg tatcggcctg gtcttcgagc ttcctgtcct
    19201 gctgttcttc ctcacgctgt tgaggatcgt caacccgtct ttcctgcttc ggaacacgcg
    19261 atacgcaatc ctgctcatcg tcattctcgc cgcggtcatc actccaacgc cggatgtctt
    19321 caatctgatg ctgttttcgg tgcccatgtg cctcctgtac tttgtgggca tcttcgccag
    19381 ctacctgctg gtgttgcatc gcgagaatcg ccggttcccg tggggcgtaa cgctgctcgt
    19441 gctcttcgcc atattgcttg tggctgcggg cgccgtctac ctcgcgatag caaagtacgg
    19501 cttccatctc gtgccgaaat ggccgtttct gattcgctga caccgcagcc tgctggattc
    19561 agcggattcg ttgttatacg atatctgcga taccggcgga gatgccgctc gagtcgcgga
    19621 catggaccta tacaaagcca ttcgtgagct ttacgaggag aagaagcggc ttgaggaggc
    19681 gatcgcctcg cttgaggaac tgctggaggc gaaggccgtc actgggaatt tgaacctgga
    19741 tggtcttaga aaacgccgcg ggcgtaagag catgagcgcg gaagaacggc gtattgtgtc
    19801 ggaccggatg aagaaatact gggcacagcg tcgcgccaag ggcggcaagt ccgcctgacc
    19861 cctcggcaca tagattcgtt aggacgtctc cttcttgaag taccacaacc agaaatactg
    19921 ctcgcccgcc ccgtgacggt aacgcggatc gaaaccgcag cgtagggcca tttccaccgc
    19981 atcccggtca gagatgggca ctccaagcca ggtgtcttcg ggcgatgtct tcagtgcaat
    20041 attcccttgg acctgaaact tgaacagtgc gcccgaccgc agcaaccggt ggacttcgcg
    20101 aatgtagttt tcgatgattt ccctgctagg aatgtgttgg aaaacaatgc tcgagaatgc
    20161 gaagtcaaac ggaaggtcgg gtagaacaga caggtccatt ccgttgttct tgtggatatg
    20221 aaccttcggg cgatccttga gtgcctgccg ggccagctcc accatctcgc tgctgacgtc
    20281 cacaccgtga acttccccga aaatctccga gagggcgtgc gtgacacggc ctgcgccaca
    20341 gccgatttcc agcacgcgca tttgtgcggg atc
//
LOCUS       DATIFQ010000404        41265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2240764, whole genome shotgun sequence.
ACCESSION   DATIFQ010000404 DATIFQ010000000
VERSION     DATIFQ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 41265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41265
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2240764"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..549
                     /locus_tag="VLE22_19835"
     CDS             121..549
                     /locus_tag="VLE22_19835"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSB16712.1"
                     /translation="MTRQGSARWEGALRDGKGTLTTESGALLKTPYSYPSRFENSAGT
                     NPEELLAAAHAGCFTMALTAQLGSSGVKPDSIYTTATVTLEKQPSGYMISGVHLDVTA
                     RIPGIDALLFERAAAQAKATCPISTVLKTKITLEARLSAM"
     gene            complement(572..2215)
                     /locus_tag="VLE22_19840"
     CDS             complement(572..2215)
                     /locus_tag="VLE22_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16713.1"
                     /translation="MRPHLLRFLLLAILAAPGYGSEGELWKTARANGTRVDDIFSRTR
                     KMLHAWLSYADRETLLLPDRLPGLIRGKANVESAYTPHNSGADNYPYLILTAFFTDPQ
                     VYRGRMIDMLRNEIRFTNAAGSIPGNLDFKTRKLGPPSFFGAGEYAKDGLVSVTELLG
                     RTPWFHRMVDMTADFAAHAPVETRFGNLPDDGAELNGDVLQTLARLIPMTADPRFLRW
                     AEEIGDAYVQEILPANGYLPGYRWDFRKLSDAGHTRFRDHGNEVIVGLVLLHALETDM
                     GRERGKTWLPVIRRMLDRVLESANPDGMLYNEVANRDLAPRNDRLSDNWGYVYAAVYA
                     FYQVTGDTKYRDAVRRVLTNLPKYRNYDWESGSMDGYADSIESALYLVAREPVPEALD
                     WIESEMKTLIAYQRPDGTIERWYGDGNWNRTLLLYAMMKTQGCYLKDWAPDVELGAVR
                     DGERLLVSVHSPKPWSGRVAFDYARHRRVLNLTKNYARLNEWPEWHTVDENTLYTVRD
                     AISGKEEIRLGSELIEGIDVAVPAGAAKKVIVSPVRRAR"
     gene            2251..3303
                     /locus_tag="VLE22_19845"
     CDS             2251..3303
                     /locus_tag="VLE22_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16714.1"
                     /translation="MRHLTWISLALCLMAVTRCGAAGNGQAEIVDAKAIWGRAPHNAF
                     TDLARFKGRWYCTFREGQGHVSPGGELRVLSSADGEVWGSSALLSMPGADLRDPKLSA
                     TPDGHLLLMAAAAWPPPAEVTHRSVTWLSSNGKEWSGPAEVGDPNVWLWRVSWHMGRA
                     YGLGYSTTADRFVRAYTSRDGTRFTSLADRVLDSGYPNESSILFLPDNTALCLLRRDG
                     DDATAQLGKAVPPYRGWTWQDLGVKIGGPNMIRLPDGRIVAAVRLYDRRARTSLCWLD
                     PEQPRLDEFLTLPSGGDNSYPGLVFHDGLLWVSYYSSHEGKSMIYLAKVRIPPAEELK
                     PERKPAWQPRHSAMGR"
     gene            3306..4289
                     /locus_tag="VLE22_19850"
     CDS             3306..4289
                     /locus_tag="VLE22_19850"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSB16715.1"
                     /translation="MAVGDSAIRDQLLFRRDRLQTAMPGAPTQARLAQLLDEVDAALT
                     RLDDGTFGLCATCHDPIEPDRLAADPLVQYCLDHLTPDQRRALQMDLDLASHIQRGFL
                     PERTVNTAGWEVHYHYEPAHAVSGDYCDVLRASPDGLFFLIGDVSGKGVSASILMAQL
                     HAIFRSLTSNHLPVDELVARANRIFCESTLAAYYATLVCGRVDRSGRLELCNAGHCPP
                     LLVRGSEVTKIEATGLPVGLFCSGHYSAKGLQLSAGDMLFLYTDGLSEARNRADTEYG
                     EERLAELLCARPGLSPHEVTSECLKDLAKHLGTAPKTDDLTIMALRYSGKA"
     gene            complement(4321..5238)
                     /gene="add"
                     /locus_tag="VLE22_19855"
     CDS             complement(4321..5238)
                     /gene="add"
                     /locus_tag="VLE22_19855"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HSB16716.1"
                     /translation="MGNFYHALPKAELHVHLEGAIEPETLLELAPVLSLEQIRERYEY
                     TNFLEFLKSFKWVVDQLGTPEQYALATRRLLERLESENVQYVEIIFSVGIAIWRGLEI
                     APIFDAMRREALDSRVTVWWLFDAVRQFGVEHVNRVAELAAERTGDGVVGFGIGGDED
                     RGPARLFGEAFGIARKAGLHLTAHAGETGGPDSVWAALELGAERIGHGIRSIEDPVLM
                     KHLRDHNIPLEVSISSNVCTGSVGSLRQHPVRRLYDAGVPITLNTDDPAMFHTTLTRE
                     YEIAARELGFAEYELRHIAENGFRYGFRS"
     gene            5259..7436
                     /gene="glgB"
                     /locus_tag="VLE22_19860"
     CDS             5259..7436
                     /gene="glgB"
                     /locus_tag="VLE22_19860"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HSB16717.1"
                     /translation="MKQSEIEAIVGGYHGDPFGILGPHADAKNGNSARWEVRAFLPQA
                     AEASTLIGGAAWPMEKLQRDGFYVAVLDDEPKSYRLRLKLWNGHTVEIEDPYRFPPLL
                     SGYDLHLHAEGTLHEAFHTLGAHLVQCDGVTGVRFAVWAPNAEVVSVVGDFNEWDTRR
                     HPMRLREGGVWEIFIPEIGVGRAYKYFVRSRVHGHRQMKADPYAFQFETPPKSASIVS
                     NLDSYDWGDSAWMDARAQRDLLKAPVSIYEVHLESWLRGPHNKILSYRELADKLVAYV
                     KQLGYTHIELLPIMEHPFAGSWGYQVIGFFAPTSRFGPPEDFMYFVDRCHQAGIGVIV
                     DWVPAHFPKDAHGLAWFDGTALYEHADPRKGEQRDWGTLIFNYGRNEVRGFLISSALF
                     WLKRYHIDGLRVDAVASMLYLDYSRKPGEWVPNQFGGNENLEAIDFLRKFNELTHQVP
                     GAITVAEESTAFSGVSRPVYLGGLGFTMKWNMGWMHDMLKYFSTDPVYRRHHHQNITF
                     SMLYAFTENFVLPVSHDEVVHTKRSLLSKMPGDEWQRFANTRAFLSYMYAHPGKKLLF
                     MGSEIGQYEEWNHDASIRWELLEFDYHRKLQALVRELNRIYRSEPALYEVDFQWNGFE
                     WIDLSDVDNSIISFLRRAADPEDLLLFTCNFTPVPREGYRLGVPAAGYYREVFNSDSE
                     LFGGSNMGNAGGVNARPIPCHGRQDSIQITLPPLAVVAFKRRM"
     gene            7464..9047
                     /locus_tag="VLE22_19865"
     CDS             7464..9047
                     /locus_tag="VLE22_19865"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSB16718.1"
                     /translation="MQAGFDVLIIGGGHNGLVAASYLARAGRRVLVLERREMLGGCSV
                     TEEVWFGYRVSTAAYLTSLLEERVIRDLELEKFGYQVDAKDPAFFSAFPDGRHFFMWQ
                     DRRRTLDEISKFSRRDAERYPAYEDHIERLSRIVAPLLLTTPPQFPPHGAAEFIDYLK
                     LAAKLYGLARRDLIPLVRIFTQSAADFLNEWFESEELKVTLATDGVIGANGGPRSPGT
                     AYILLHHCMGGVGGKRGLWGFVRGGMGMVAESIAASACRFGATLRTSSGVEKILVRDG
                     RACGVVLSGGEEIAASTVVSNLDPKRTFLGLIDERGLDPEFAAAIRAFRCEGTSLKMN
                     LALSGLPEFRALPGAPGPQHRATMHICPSIDYVERAWDDAKYGRPSEWPMLELTIPTM
                     YDASLAPPGRHIMGVFLQYAPYTLRDGNWDQLRESYADRVIDLIADYAPNIRDIIVDR
                     QTLTPLDLERRFGITGGNIFHGEMSLDQMFVMRPVAGWARYRTPIRGLYLCGSGAHPG
                     GGVMGAPGYNAAREILKDT"
     gene            9144..9998
                     /locus_tag="VLE22_19870"
     CDS             9144..9998
                     /locus_tag="VLE22_19870"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HSB16719.1"
                     /translation="MTTDVIRMPHDRALNWPTTTAFALFHLGAVAALFTFSWTSLAAA
                     VFLYWMTTGLGISMGYHRLHTHRSYRVPVFLERFFAVCGALTLEGGPISWVAIHRIHH
                     QKSDQPGDPHSPRDGAWWAHMGWILFGETNHNNTRGMSKYAPDLAKHRFYVWLNNYHW
                     VPTVVLGLLLLAFGGLSLMLWAVCLRVVFGLHATWLINSATHMWGARRFATRDDSRNN
                     WWVALITFGEGWHNNHHAHPTSARHGLAWYEFDPSWIQIRLLSLLGIAKSVRVARVTD
                     STAGRQAA"
     gene            10048..10941
                     /locus_tag="VLE22_19875"
     CDS             10048..10941
                     /locus_tag="VLE22_19875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB16720.1"
                     /translation="MVTAGRRKSIAFVISLGAGLVLVILLLYIGWVLLGWRTGILLFF
                     GVLMLAMVISGIVINTIFLVREIRRNEQHNAFINAVTHELKTPVASIRLYLQTLQKHE
                     VAELKRNEFYRIMLDDSDRLLATIEQILRTGRLASTRHGLNVAPIDLGGVVEECLARA
                     RTLHNASAQSLRYSRGPQVTVLGDPDDVQAAVSNLIDNAVKYSGSDVEVTVEPPETGG
                     KYVSVRVKDHGPGIPKSELNRIFRRFYRIPGPIAARVKGTGLGLFIVRSVAKRHGGRV
                     WAESEGPGRGSTFVLQFPVSK"
     gene            10938..11663
                     /locus_tag="VLE22_19880"
     CDS             10938..11663
                     /locus_tag="VLE22_19880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB16721.1"
                     /translation="MSRILVVEDEQHLAEGLRFNLEAEGYQVDVVETGEAALDLLVEH
                     PAAFDVVVLDVMLPGVDGFTVMSEMRRSGQFVPTLMLTARGHPEDVLRGFAAGADDYL
                     PKPFELVILIARIHGLLRRREWLRASQHSDTSSFGSRDTYAFAGKSVDFDRLELNVRG
                     QVFQLTLMEANVLRYLTSHEGTPVSRKKMLEEVWGLHVDTDTRAIDNFIVRLRRYIED
                     DPTRPRHLLTVRGIGYRFIASPK"
     gene            complement(11694..12341)
                     /locus_tag="VLE22_19885"
     CDS             complement(11694..12341)
                     /locus_tag="VLE22_19885"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HSB16722.1"
                     /translation="MTYCWMHPPLIVLPMRKALDHLRRADPVLARLIGEIGPCRIRFI
                     EPGFEALAKAIVYQQLSGKVAATIFGRLAAAAGDGRVTPKAVLTLEPAKLRELGLSRQ
                     KIAYIRDLAERVLTGEIDFPAMERLSDQEVMESLTKVKGIGVWTVHMFLIFALRRPDV
                     MPVGDLGLRAAIRRAYALETLPSPAEMEEMARKWRPYCTMASWYLWRSLETDANL"
     gene            12351..13322
                     /locus_tag="VLE22_19890"
     CDS             12351..13322
                     /locus_tag="VLE22_19890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB16723.1"
                     /translation="MRPLNLFAVAVAAMLIAPAQEPQETPTIKVEVDVVNILCSVRDK
                     RGSLISTLGKDDFVVKEDGKAQDIRYFARETDLPLTLGLLVDVSKSQEHLIEIERRAA
                     AQFFSQVLRKKDMAFLISFGPEVELLQDYTNSQTLLRNGLDELHVRGAVGGPLPGPVP
                     TISQPRGTVLHDAVYLASNEKLRGEVGRKAIVLITDGIDTGSRVKLEQAVEAAQKADA
                     IVYSIYYYDPSAYYQGGFSFGGGNDSALRKMSEETGGRVFKVSKKNTLDDIFKQIQEE
                     MRSQYSIGYSPANPAKDGSFRRLEVDTARKDLKVQARKGYYAVKAGS"
     gene            complement(13306..14193)
                     /gene="rfbD"
                     /locus_tag="VLE22_19895"
     CDS             complement(13306..14193)
                     /gene="rfbD"
                     /locus_tag="VLE22_19895"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HSB16724.1"
                     /translation="MPRKVLIFGNAGQLGVELVREFTNRGYRVAGYDRGEVDITEASR
                     VEDCVAQFDPAVVINAAAYNQVDVAEKEPAPAFAVNALAVQNIAVACRQVGARLVHYS
                     TDYVFDGTLGRPYTEEDSPRPLNAYGVSKLAGEFFARAYLERPLLIRTSGVFGPGGLA
                     TARGNFVEVMLRIVTTGQPIRVVEDHFASPTYAPALASRTADLVEKNLSGLFHIGGGM
                     ATSWFEYAKVIFQAAGLTPRLHATNEREYRTAARRPKYSALSNAKVEAAGIAPMPPLS
                     EAVTDYLNRREGLLTKSRP"
     gene            complement(14232..15032)
                     /locus_tag="VLE22_19900"
     CDS             complement(14232..15032)
                     /locus_tag="VLE22_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSB16725.1"
                     /translation="MAFCTNCGASVSGTFCSQCGTPVSGGTPSTGAATDIQAAARGQV
                     PAKRRMGPLGWVLIVVLGLFVLGTLAVVGGGLFVYHKAKQAGVDPELMRSNPTLAISK
                     MIVATNPDVEVVSVDEGKGLITLKEKSTGKTVTMNFEDVKRGKIVFKEEGGKGGTATM
                     EFGTGSDKLPDWIPPYPGAKAEGRFVMKGTEGEGGTFHFTTGDAAVTVIAFYKDAFKQ
                     GGMNVTTNESGEGGSVTGTDEAKGRTVAVILNPGSGETTVNITFGTKK"
     gene            complement(15053..15412)
                     /locus_tag="VLE22_19905"
     CDS             complement(15053..15412)
                     /locus_tag="VLE22_19905"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB16726.1"
                     /translation="MDLRSEPRFPADEPVRVTVLSGAKLETDGKIVNVSGRGMCLRIR
                     ARVPVGAAVRVDIDGSLLLGEVAYCRTDGDLYAVGLQLEQALTISEELSRLMQRLMEE
                     SNGPAAERTVASQTRMA"
     gene            complement(15446..15814)
                     /locus_tag="VLE22_19910"
     CDS             complement(15446..15814)
                     /locus_tag="VLE22_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16727.1"
                     /translation="MNRRRRLLTKSTVFVSVGETTEVYPSIDDVPADVRRKLIDRPGE
                     WESATIVIADRGGREEVVRALRGQHSAVALRILNGSEEPDQTFVSRAIDWVYRRRHWL
                     AATTAAAAAITIWLVLSRLL"
     gene            complement(15811..16710)
                     /locus_tag="VLE22_19915"
     CDS             complement(15811..16710)
                     /locus_tag="VLE22_19915"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSB16728.1"
                     /translation="MDLDQLHTFLEIVRLKSFSKAAETCFRTQPAISAQIRQLEGELN
                     TSLFDRLGSRIALTPAGRIFREYAEQILDLRRRAQDAINELDRVPRGALVIAANEATC
                     IYVLPQVFSEYTKRFPNVQLMVDRSYGKRVVEAVMENQADFGFTQLPVQDRKLQVVKI
                     HSDEIKLISLAGHPLARKEYVSCQDLAGCSLILPKTGNTRSRLDAWLELVEDIIHITM
                     ELDSTEMIKRFVIAGLGVSFMAASHCREEVDSGKLAAISLAPEPMIRHVGLIYRKDKA
                     LSRAALGFIDSVLEHAACGVISR"
     gene            complement(16808..17698)
                     /locus_tag="VLE22_19920"
     CDS             complement(16808..17698)
                     /locus_tag="VLE22_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16729.1"
                     /translation="MKTLGRLAIAAILLATLLQAQPQVALLDSANTKEFFRLHYNGCP
                     FYLPDGEEYTRYFMGWQYALSQSPDIPFTVIHDADVTEDGLALFKVLILSNAASLNKQ
                     QTEVIHQWVVRGGRLLATFGSGYKSTRLDPREADGLRRQKGHTFGLHELWHDPMTTVF
                     SSFNLASGVDIRVTEFAGPTGCSDVESTLSPLDNVLPYGAEANILVQRPENYPGVLGY
                     VQFLETTDWSRPTPAILSTRAGRGRVVYYAFAPEFVVSLAYVAAAQNPSCTHDSGAFA
                     NRADKALPLMRCTVSWLLGN"
     gene            complement(17717..18532)
                     /locus_tag="VLE22_19925"
     CDS             complement(17717..18532)
                     /locus_tag="VLE22_19925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HSB16730.1"
                     /translation="MLLNSAGRVCLALFGVAGCFAQDAGNAGQSDVLPVRLELGAYGS
                     QLNRNLGTWRGLQGQLWIRSNPRFVPALIFDTQTRPEGTQQNYGFLSYLNWSKSFYTV
                     QGFSAAPQRNERSIFFPKRRYDIKAYWKVPPTRNFVLGAGFTRFDYGGPGHGQIFNAG
                     ALYYHKKLVMEGNLFVNRNQPGDFVSAAGTLAAQYGTEGNYWFGVTAGGGREQYRFTD
                     ITPAEIRLLSYSINTFYRKWLSRHVGFVVSFDYQDKLTAYRRAGLSTGLFFEF"
     gene            complement(18544..19722)
                     /locus_tag="VLE22_19930"
     CDS             complement(18544..19722)
                     /locus_tag="VLE22_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16731.1"
                     /translation="MIPWRFLIFAGVLALILYLHFYLAHRQWRRLKGRPSLEIDVTYV
                     RMEDCLPQSFRLKVKEWLQLPALTVTGTERTILKGHERIRATGPMAIGDRHESDAIWV
                     VDGDFSCGSGCEFFREIYTRGSARIGHGSRLQSLAADGNAVLEDGVTAARWLDSTGEM
                     ILGTDCRVGARVTSRTAVRLGMGCRVLSVFAPRVSTPGSNGSPSSQDLPPPDDLVEIP
                     PPDESSTAEQALLDAGVDPKKVVRMSADSWHYAGDLTPSRPLRVGAKLIVKGRCHLPP
                     RSAVRHDLKSDRSLHIGRGGFCEGNLVAGGDIYLAPDCRFAAVVHAFGSLLISEGTRG
                     AGKEAPVVAYAGDGIWVENNVVVCGKLASPGGVMVVDAAGAEDWRKRRKIQTSRLLRV
                     "
     gene            complement(19719..21083)
                     /locus_tag="VLE22_19935"
     CDS             complement(19719..21083)
                     /locus_tag="VLE22_19935"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB16732.1"
                     /translation="MTILILVAKGCLLFAIAVLALYGLRHYTLALYRLSIRRPRDMME
                     ITGYFLPRTSVLVPMHNEEKVAADLLQALVEGDYDRDRIEILAIDDRSEDRTGEIISD
                     FAARYPVIKAIHRRDGEGGKAAALELATRQASGEVLLLFDADYFPGRAMVKQLTAPFC
                     DPEVGAVMGRVVPHNSGSTLLAALLGLERAAGYQVGQQARYNLGLTPQFGGTVGGVRA
                     SALRAVGGWNTKSLTEDTDLTFRLIAKGWKVAYVNRAECYEEVPEAWEVRKRQIARWA
                     TGHTDCLHRLWSSVSGSKFLSRPEKVDALFVLGCYLTAPILVLGWLASLVLFFSPEAS
                     AVPPLLLALAFAGYQVFGNQATFFELGAAALLDGDARRVLLIPVNLFNFFASTAAICG
                     ALLRYYVGRVWGSRGPRWHKTKRFRENSNGEQFRNGNGSGRGLFSQRPSGAYTYTFST
                     ESEQ"
     gene            complement(21080..21889)
                     /locus_tag="VLE22_19940"
     CDS             complement(21080..21889)
                     /locus_tag="VLE22_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16733.1"
                     /translation="MTVSLKVRMPAFLATPHSHMRREFIYPHRSVRSFRVTVAKLLSA
                     VFTAAVLCGSIAAYNAEFFTWHDRLCVTLLEWTGVPVTGEQTIELFEGLSTAVVPAVP
                     VARFESNPLRFVLLAVAGLLALWAIQHRIALSRGFVLFLAALLLITACVVAIHPSSQF
                     GAVEFGQIWLRGEVLVWLLLPVFSAAMFTLVQPIWLIGVTLTVLVQVFGIFWSAVRMA
                     FCLAVMHYSGILFVPILWFALGVLADMIYLMVFYSMAVHWNASRYWRRKAL"
     gene            22051..22698
                     /locus_tag="VLE22_19945"
     CDS             22051..22698
                     /locus_tag="VLE22_19945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB16734.1"
                     /translation="MPRNKLALIALAPAVIWFTACGPDKKEEPKVVKAPEQFRVKFET
                     TKGDFVVEVTRAWAPRGADRFYELVTERYYDGCKFFRVVPRFVVQFGLNGNPALSQKW
                     SQMTIPDDPVKAHNEKGTLSFAMAGPATRTTQIFINLNDNIHLDRSNFAPFGKVTSGM
                     EIVESLFKGYGDAPPQGVGPVQALIREEGNTYLERHFPRLDGIKTATVEPVEGGA"
     gene            complement(22797..25199)
                     /gene="hrpB"
                     /locus_tag="VLE22_19950"
     CDS             complement(22797..25199)
                     /gene="hrpB"
                     /locus_tag="VLE22_19950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HSB16735.1"
                     /translation="MRAPLPVDTLIPDILSGLRESPSLVLEAPPGAGKTTRVPPALLG
                     LGCGDVLVLEPRRLAARLAARRVAYESGQKVGQTVGYQVRFEEVAGPRTRLRFLTEGV
                     LARRLLSDPELRGVDTVVLDEFHERHLDGDVALALLRRLQRGPRPDLRLVVMSATLET
                     APVAAFLGRCRVLRAEGRLFDLALSYTPVSAATLAEQVASALESLLREGLKGDVLVFL
                     PGAAEIRQAARTCGPIAERAGLLLLPLHGDLSPEEQDRAIAPADRRKVILSTNVAESS
                     VTIEGVRAVIDSGLARVASDSPWSGLPTLRVARVSKASATQRAGRAGRTGPGRVIRLY
                     PAEDFHRRPEYDAPEIRRRELSQTALEVRAMGAGSLADVEWLDAPPAAAVEAAEQLLR
                     RLGAVDAGRRLTEAGRVMSRLPLHPRLARLVAEADRRGSGDDGCVLAALLSAGERLPL
                     ETHHSGPSDLLLLLDSEWGPYTRRVYQQIRRSARPERQKKHDETALLASVLAAFPDRV
                     ARRRQGDELLLAAGGSAVLSPLSVVRARDWMVAVDIEERRERGLPLVRLASAIEPEWL
                     LDLFPDEVSERNAVEWNRNAERVDAVSALVFCSLVIEESRNAQPEPATAAELLARKAL
                     EAGVGRFADAQEVEGFLARVAFASEHTGIPSLGDEDVKAAFESLCDGLRSFTELEQVA
                     RGGLLRALERRLPPDARRLLREAAPERIRLPGGRQVPVRYARGQPPWIASRLQDFFGL
                     RETPRVAGGKVPLVVHLLAPNHRPVQMTADLAGFWDRLYPQVRRELSRKYPRHSWPEK
                     PP"
     gene            25329..26423
                     /locus_tag="VLE22_19955"
     CDS             25329..26423
                     /locus_tag="VLE22_19955"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HSB16736.1"
                     /translation="MGKQIVGHLDPFFFEVVEDIRKLLGLVFGTSNTFNMAISGTGSA
                     GMEAAVANFVEPGSKFAVFANGFFCDRITEMGHRYGANVVRFEKPWGETFDDGEAAGF
                     IERERPAVVAFVHAETSTGALQPGKAICEAAHRAGAIVIADCVTSLGGMPVKIDEMGV
                     DVAYSCSQKGLSCPPGLAPVTVSPRALERLRARTTPQRTWYFDLKLLDEYFEGAHRYH
                     HTAPISMFYALREGLAIIAEEGLENRWERHRRNHLAFVAGVEAMGLRMHVAEDRRLWV
                     LNTPRVPDGVEDLKVRKRLMGKHGIEILGGFGPLAGKVFRVGIMGASSTRENVLLLLD
                     ALEEALRTEGYEPKAGGRAAAEQVYAAPVG"
     gene            26433..26810
                     /locus_tag="VLE22_19960"
     CDS             26433..26810
                     /locus_tag="VLE22_19960"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HSB16737.1"
                     /translation="MRRRTCLKTLAAAAGVSSISAAAPPIQLHVDLDVDSAKEKKLVE
                     NFRNVFRPAISRQPGFVDVKLLRLRKALSGPTPLPYRLLISFQTEEQRQAWVATTDHQ
                     RAWPAIEENLRGSKYTAVLYDAD"
     gene            complement(26864..27412)
                     /locus_tag="VLE22_19965"
     CDS             complement(26864..27412)
                     /locus_tag="VLE22_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16738.1"
                     /translation="MRVMLLCVLLALCILAPGCLVRSLQPLFTPSDLAVEPRLLGTWQ
                     AEGDDETWTVIGSGPKTYEVICRDGNKSTYLEGRLGKVEEILFLDITPKKSDDAFSVP
                     AHVFVRLEISGDALRAGVLKPEWLADGLREKRVQIGHTLVRIDDEPSIVLTASTEEIR
                     GFLKRVVREENAFSSGEYRRVK"
     gene            complement(27435..28424)
                     /locus_tag="VLE22_19970"
     CDS             complement(27435..28424)
                     /locus_tag="VLE22_19970"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HSB16739.1"
                     /translation="MRRAKISALGCYTPPRLLTNTDLEKLVETSHEWIMERTGILERH
                     IAEPGMATSDLAVEAAREALAQRGIEAAELDAIIVCTVTPDMFFPSTACLVQDRIGAK
                     GAWGFDLIAACSGFLFGLTTAAHFVAAGTHKKVLVIGADTMSRIIDYTDRATCVLFGD
                     GAGAMLVEPTDGEDQGFVDFLNEVDGSGGDFLKMPAGGSRQPASVETVENRLHYVHQE
                     GQQVFKYAVRKMYELCNQLLERNNISARDIRLMIPHQANRRIITATADRLGLSDEQVV
                     INIQRFGNTTAGTIPLATRDALAEGRLKKGDLVLFAAVGAGYTSGVGLWRWAY"
     gene            28470..29195
                     /locus_tag="VLE22_19975"
     CDS             28470..29195
                     /locus_tag="VLE22_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16740.1"
                     /translation="MVHSGGMQVESALLFETPAEIWARVYRELRPRSRMPEITIEFRQ
                     FAGANTFARLEGGLLRVRITDVLEGAPAPVLEALAYILLSKLFRRPVPRIYHHRYRMY
                     LNRKEMRRSLHLLRQIRGRKLLSGPQGDHYNLETVFEDLNLRYFHGLMARPLLSWSHR
                     PSRSILGHFDPSHNAIVISRLLDSVSIPKIVLAYVVFHEMLHLRHPVEHAGARRRVHT
                     RAFREAERVFGGLKEAKEWLKRL"
     gene            complement(29288..29821)
                     /locus_tag="VLE22_19980"
     CDS             complement(29288..29821)
                     /locus_tag="VLE22_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16741.1"
                     /translation="MDIAIAAALALVVATAVGFGLIFKRMLFRDRVPDFDPEWLENFS
                     VSKYRPMERLLSEEDFEFLVSQEGYEPKIGKRLRSERRGIFRGYLRSIRRDFQRLEAA
                     VRLFMVDSAEDQPELAKALLKQRLHFTYAIWSVEVRLALHWLGIGTVDIRQLVGALDV
                     MRVHLGQMALARQASVA"
     gene            complement(30049..30828)
                     /locus_tag="VLE22_19985"
     CDS             complement(30049..30828)
                     /locus_tag="VLE22_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16742.1"
                     /translation="MPEISQLEHYLWITSLFLTAILIVRMLREGLARLYAGFFLYLSV
                     GVLQTLIMLPFRPNTTTYAWLYLFTQPVIWLLSILIVLELFSLVLRRYQGIASLGRWT
                     MMAALAAAVLIALVTLVPDLQNAGGRYRTLIYYNVIERGINSSLVIFLLVISVFVVWY
                     PLPLGRNVILHAIVYSVYFLCSTLALFVQNIRGLEITRIISTILIALTCACLVIWILF
                     LRRQGEEQKASVRRQWRPEEGEQLVRQLDAINAALLRSSRK"
     gene            31246..31704
                     /locus_tag="VLE22_19990"
     CDS             31246..31704
                     /locus_tag="VLE22_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16743.1"
                     /translation="MKARKLKGSFERGPAADLWRKTLVQIPSVFGRLVYLASLRDQNT
                     GRYEHHGMSQVFGEDEADQSLRESHGRTFADWLCFGLEQQKADLDLYLSSFEPDKRTI
                     LETWIRLAPYRNLVPASVREVERRLYLTDLEALLDLLKNEYAVVSPDPDA"
     gene            complement(31661..32443)
                     /locus_tag="VLE22_19995"
     CDS             complement(31661..32443)
                     /locus_tag="VLE22_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16744.1"
                     /translation="MEDAGQKLKRVRERLNLRYRDVEEASLRIAEKHRNSEFVIALSR
                     LADIENKGTLPTLYRLYSLCAIYRLDFAEVLTWYGVSIAGLPADAASVQVERTHLIGF
                     EANGQGEMQLPLALDPGIDLRRTTYLSRLIQRWGKLPLMMLNGIDLRSHRYAFIGTED
                     WAMYPLIQPGSLVLIDETKRKIVNSGWTNEFERPIYFLEHRDGYLCGWCNLNESQLVV
                     QPHPASMCNPEVYSYPDEIEVVGQISGVAMRLDQGKRRRTHS"
     gene            32623..33177
                     /locus_tag="VLE22_20000"
     CDS             32623..33177
                     /locus_tag="VLE22_20000"
                     /inference="COORDINATES: protein motif:HMM:NF004685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HSB16745.1"
                     /translation="MIVGISGASGSIYGLRLLEKLRSRPSVEIHLIITRAGERTIFLE
                     TGKVAADVKSMAHFSYPVEDIGCRLASGSYLADGMVVAPCSIHSMAAIATGVTDNLLV
                     RAADVCLKERRKLILMVRESPFHLGHLETMAALARIGAIIAPPAPAFYHKPETVLDIV
                     DHSVDRVLDLLGLPDVQAKRWQAT"
     gene            33199..34056
                     /gene="pheA"
                     /locus_tag="VLE22_20005"
     CDS             33199..34056
                     /gene="pheA"
                     /locus_tag="VLE22_20005"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HSB16746.1"
                     /translation="MAKRPMKAAFQGERGAFSQQAVRQLLGDGAEVLPCQRFEEVFQS
                     LRARKVDAAVVPIENTLAGSVHENYDHLLHFDDFEIVAETSVRIVHNLIASPGVTFRK
                     IRRVFSHPVALNQCLDFFTNHPDIERVPFYDTAGSVKMIMEDGLKDAAAIASALSAEI
                     YGAKILKRSIEDDRRNYTRFFLLRRRDAARLAATNRSAARGWKTTLVFTTRNIPGTLF
                     RALSAFALRDINLMKIESRPLRGKPWEYLFYLDFLGDLDEPRVQNALSHLSELADFLR
                     VLGCYPRGE"
     gene            complement(34085..35488)
                     /locus_tag="VLE22_20010"
     CDS             complement(34085..35488)
                     /locus_tag="VLE22_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16747.1"
                     /translation="MPRSALVAFFVCAFAMCVLAQPRPPAGLLRPSQSNPRYFTDGSG
                     RAVYLTGTHNWNNFVDTGHRSRIGEPPPVFDYSRFLDFLDAHHHNFFRLWRWESPKWT
                     DDEPAGVAYNALHPWLRTGPGLAADGKPKFDLTRFDPQYFDRMRARIIAAGARGMYVS
                     VMLFEGYEIQFYDAWNCHPFNRDSNINGIDADTNGDGRAPEYNTLEDSPTGRKVLALQ
                     EAYVRKVIDTVNDLDNVLYEICNEAGSYSTDWQYHMIRFVHAYEASKSKRHPVGMTFQ
                     YKGGTNRTLFESPADWISPNRGDGPGDYLGDPPANSSGKVIVNDTDHLCGHTCGDNVW
                     VWKSFTRGLNVLFMEELTPSPTWHDSARAAMGQTRLLSERIDLAGMSPHDELASTRYC
                     LAKPGSEYVVFQPGGQGEFRVDLRGVKGAFAVQWLNVNSDKSSSGGHVEGGEFRTFRT
                     PFPGPAVLYLKRADGGQ"
     gene            complement(35538..36437)
                     /locus_tag="VLE22_20015"
     CDS             complement(35538..36437)
                     /locus_tag="VLE22_20015"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSB16748.1"
                     /translation="MPGRSVFRDSVEYALASLVLKSLACLPRWPAERLARGYARLLDL
                     VLPRLRRVARRNLSMALPGLSDGQREQVIDGVFRSIARLLAAFAQFPKMGRENVSEWI
                     RYEGFEHFEEALRRGKGVLFATAHLGNWELSAFAHALMAAPMHVVVRPLDNPKIDALV
                     SSYRTASGNHVIQKKDFARGILKALSANEAVGILIDQNTGLEEGVFVDFFGIQACANP
                     GIARLAAHSGAAVIPGFALWSDVERRYVLRFYPPVEITGDPAADTQRLQSSIEAVIRE
                     HPDQWLWIHRRWKTRPKGAPALY"
     gene            complement(36442..37548)
                     /gene="mnmA"
                     /locus_tag="VLE22_20020"
     CDS             complement(36442..37548)
                     /gene="mnmA"
                     /locus_tag="VLE22_20020"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HSB16749.1"
                     /translation="MPEKITAVAMSGGVDSSVVAGLLRRRGENVVGLTMQLWNQRRLP
                     ELAGENATGRCCSLDDVYDARHVAETLGIPYYVVNFEERFEEQVVRPFIDDYLAGRTP
                     IPCTLCNNHIKFDQFLELADSVGAEKIATGHYARVSQDEKSGRYLLWCGADDAKDQTY
                     FLFGLSQAQLARTLFPLGGYTKDQVRGMAEEMSLPVARKGDSQEICFVPNGDYGAFIE
                     AFFREQGIPSRQTRGEVVGTDGRVLGEHAGVHHFTVGQRRGLGVAAGEPLYVIATVPA
                     TQRVIIGRSEELFRDTLSAREVNWFPWASLSEPVRARVKIRNKHAAAPAMVSPTGEPA
                     RIEVRFDEPQRAVTPGQAAVFYDGDLVLGGGWIE"
     gene            complement(37541..38698)
                     /locus_tag="VLE22_20025"
     CDS             complement(37541..38698)
                     /locus_tag="VLE22_20025"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HSB16750.1"
                     /translation="MDRFYFDHNATTPVSAEVLEALVPLLGEVYGNASSIHHFGQVAK
                     QRLETARRQTAALIHARPREVVFVSGGTEADNLAILGAVRRSPKPRKHVVTTAIEHPA
                     VLNTCGQLEREGVEVTYVRAGSSGVVDPDEVRRALRPDTVLVSVMHANNELGTIQPVG
                     EIARIARDAGALMHSDGVQAAGKTPVDVAALGVDLYSMSGHKIYAPKGAGSLYIRKGT
                     AIEPVLFGGHHERDRRPGTENVPGAAAFGAAAEWILAHLSDEAVRIGALRDRLEQGIL
                     ARVPHAHVNGAGSPRTPNTTNIRFDGIEGEAMVIALDLMGFAVSSGAACSSGAVEPSH
                     VLTAIGLSAMDAKSSLRFSLGRSNTEEQVDALIGAVAASAARLRKLSPAYA"
     gene            38724..39512
                     /locus_tag="VLE22_20030"
     CDS             38724..39512
                     /locus_tag="VLE22_20030"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HSB16751.1"
                     /translation="MPRGVITLTTDFGLSDHFVGTMKGVILSIHPQATIIDISHGITP
                     YEISEAAFVVAQAYRYFPPGTVHVVVVDPGVGTARRPILIEAAKQFFIAPDNGVLSMI
                     CSREKHRARAITAKKYFRKPVSQTFHGRDIFAPAAAHLAKGVALARFGKPISDYLRST
                     FEKPVRTGKRIWTGTILKIDRFGNLITNFHIDEFPAVRERMFEMTVGPRRLSYLANNY
                     TECGFGELFLIVGSSGYLEVSANQGSAAKMIGCATGTQVDLTLY"
     gene            39675..40484
                     /gene="mutM"
                     /locus_tag="VLE22_20035"
     CDS             39675..40484
                     /gene="mutM"
                     /locus_tag="VLE22_20035"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HSB16752.1"
                     /translation="MPELPEVEAVCRKLRRDALGACIAAARVLRSGTTRPQKTRRVES
                     LAAGRIIETIERRGKNILIGLSGGMWVRVHLRMTGNLHVIPDVHFRPASTRAYFELEG
                     GRGLIFDDPRALGRLHIHTTAEAARLMEDLGPEPLSPVFTLDALAAAAGRSRKPAKLF
                     LMDQQHVAGLGNIYAAEALFRARIHPARPMNRIGPSRLAVLHSAIIEVLQEAVHWAVR
                     SYGRPGEFSEAEDFPLAVYGREGELCVTCDRRVRRIPQGGRSTYYCPGCQR"
     gene            40662..>41265
                     /locus_tag="VLE22_20040"
     CDS             40662..>41265
                     /locus_tag="VLE22_20040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16753.1"
                     /translation="MPDRRYFLKALALGGVGLFMRGKGGPALAQIHGGTLDPSNVPKY
                     QTPMLIPPAMPKAGTIVQRGGKPIDYYEISMKQFAQQILPAGLPPTTVWGYGAVAADS
                     RRGLLLHNAPSLTIEAQWKRPVRVKWINDLVDGNGNYRPHLLPVDPTLHWANPPGGTA
                     GRDMRPEFRSTPGPYTGPVPIVTHVHGAVHVGDESDGYAEA"
BASE COUNT         7995 a        13375 c        12040 g         7855 t
ORIGIN      
        1 gagccccact actttttggc cggattgaag aattcttttc aaaagccacg aaataagtgg
       61 cttcatttgc gccgtcaaac cttagaatag actcgcagga caatacctct ggagaaatct
      121 atgaccagac agggatccgc acgctgggaa ggcgcactcc gcgacggcaa gggtaccctc
      181 accacggaaa gcggcgccct cctgaaaaca ccatactcct acccgagccg cttcgaaaac
      241 tccgccggaa caaatcccga agagctgctg gcggccgccc acgccggttg cttcaccatg
      301 gctctgacgg cgcaacttgg gtcctccggc gtcaagccgg acagcatcta cacaacggct
      361 acggtcacac tggaaaaaca gccgagcggt tacatgatct ccggtgtgca tctcgacgtg
      421 acggccagaa tccccggcat cgatgcactg ctgttcgaga gagcagccgc tcaggcgaag
      481 gcgacctgcc cgatatccac cgttctgaaa accaaaatca cgctcgaggc gcgactcagc
      541 gcgatgtgac ggagggaatc ctgctgatcc gtcaccttgc cctccggacc ggcgaaacga
      601 tcaccttctt ggcggcgcct gccggcacgg cgacgtcgat tccctcgatc aactcgctgc
      661 ccagcctgat ctcctccttg ccgctaatgg cgtctcgtac cgtgtatagc gtgttctcgt
      721 ctaccgtgtg ccactccggc cattcgttga gccgggcgta gtttttcgtc aggttcaaca
      781 cccgccgatg gcgcgcgtag tcgaacgcca cccgccccga ccaaggcttc ggagaatgaa
      841 cgctgacgag cagccgctcg ccgtctcgga cggcgccaag ttccacgtcg ggcgcccagt
      901 ctttcagata gcaaccctgt gttttcatca tcgcgtacag cagcagcgtc cggttccagt
      961 tcccgtcgcc gtaccatcgc tcgatcgtcc cgtccggcct ttggtacgcg atcagcgtct
     1021 tcatctccga ctcgatccaa tccagcgctt ccggcacagg ttcgcgagcc accaggtaca
     1081 acgcgctctc gatcgaatcg gcgtacccgt ccatgcttcc gctttcccag tcgtagttgc
     1141 ggtacttcgg taaatttgtg agtactcgcc gcacggcgtc gcgatacttc gtgtcgcccg
     1201 tgacctgata aaaggcgtag actgccgcgt acacataacc ccagttgtcg gagagcctgt
     1261 cgttgcgtgg cgccaggtct cggttcgcga cttcgttgta cagcatccca tcagggttgg
     1321 ctgactccag aacccggtcc agcatccgcc ggatcacagg cagccaggtt ttcccccgct
     1381 cccggcccat atcggtctct agcgcgtgta gtaacaccaa acccacgatc acttcgttgc
     1441 cgtggtcgcg aaaccgcgtg tggccggcgt cggatagttt gcggaagtcc catcgatacc
     1501 cgggaaggta tccgttggcc ggaaggatct cctgaacgta cgcatctcct atctcctcgg
     1561 cccatcgcag gaatcttggg tccgcagtca tcggaatcag gcgggcgagc gtctgcaaaa
     1621 cgtccccgtt caattccgca ccgtcgtcag gaaggttgcc gaaccgggtc tcgaccggcg
     1681 cgtgcgccgc gaagtccgcg gtcatgtcaa ccatccggtg gaaccagggt gtccgcccga
     1741 gcagttccgt caccgagacc agcccgtcct tggcgtattc ccctgcccca aagaaactcg
     1801 gcgggccgag tttccgcgtc ttgaaatcca ggttgcccgg tatcgagccc gcggcgttcg
     1861 taaatcggat ctcattccgc agcatgtcga tcatgcggcc gcgatatacc tgcggatcag
     1921 tgaagaacgc ggtcaggatc agatacgggt aattgtcggc tccggagtta tgcggtgtgt
     1981 acgccgactc cacgttcgcc ttgccgcgga tgagacccgg caacctgtca ggcagcagca
     2041 gtgtctcgcg gtccgcgtaa gaaagccagg cgtgaagcat cttccgagtc cggctgaaga
     2101 tatcgtccac tcgcgttccg ttggcgcgag cagttttcca gagctcgccc tcggaaccgt
     2161 atccaggcgc cgccaggatc gccagcaaca gaaatcgcag taaatggggt ctcatggtcg
     2221 cttccacgca cgtacaatag tagtaaatcg atgcgacatc tcacctggat ttcgctcgcc
     2281 ctttgcctga tggcagtgac gcgatgtggc gcggccggga atgggcaggc cgaaatcgtc
     2341 gacgcgaaag ccatctgggg ccgtgccccg cataacgcct tcaccgatct cgcgcgattc
     2401 aagggtcgct ggtattgcac gtttcgcgag ggacaaggcc acgtctcccc cggcggggag
     2461 ctgcgtgtcc tgtcgtcggc ggacggtgaa gtctggggct cctcggccct gttgtcgatg
     2521 ccaggcgcgg acctgcgtga ccccaagctg agtgcgaccc cggacggcca tctgctgctg
     2581 atggccgccg cggcctggcc ccctcccgcc gaggtgactc accgcagcgt tacctggctt
     2641 tcaagcaacg gcaaggagtg gtcgggcccg gcagaggtcg gcgacccgaa cgtctggctg
     2701 tggcgcgtct cttggcacat gggacgggcc tacgggctgg gctacagcac cacagccgac
     2761 cggtttgtgc gagcctatac gagccgggat ggtactcgat tcacttccct tgctgacagg
     2821 gtcctcgatt ccggctatcc gaatgagagc tcaattctct ttctcccgga taacaccgcc
     2881 ttgtgcctcc tccgccgcga cggagacgac gccactgcgc agctcggaaa ggccgtccct
     2941 ccgtatcgcg gctggacctg gcaagatctc ggcgtgaaga ttggcgggcc gaacatgatc
     3001 cggctccctg atggccgcat cgtggcggcc gtgcggctct acgaccgacg agcccgcacg
     3061 tcgctttgct ggctagaccc ggaacaacca agactggatg aattcctcac gcttccctcc
     3121 ggcggtgaca acagctaccc cgggcttgtt tttcatgacg gtctcctttg ggtgagctat
     3181 tattcgtctc acgagggcaa gtcaatgatc tacttggcaa aggttcgtat tccgcccgct
     3241 gaggaattga aacctgagcg caaaccagcc tggcaaccca ggcattcggc aatggggagg
     3301 tgagtatggc ggttggggat agcgcaatcc gtgaccagtt gctttttcgc agggatcgcc
     3361 ttcagacggc catgcccggc gcgcccacgc aagcccggct tgcccagctc ctcgatgaag
     3421 tggacgccgc cttgacccgc ctcgatgacg gcacattcgg cctttgtgca acttgccacg
     3481 atcccatcga gcccgaccgg cttgcggccg accctctcgt ccagtattgc ctcgaccatc
     3541 tgacgcctga ccagcgtcgg gctctccaaa tggatctcga cctggcctcg cacatccagc
     3601 gcggcttcct gcccgagcgc accgtcaaca cggccggctg ggaggtccac taccactacg
     3661 agcccgccca tgccgtcagt ggcgactact gcgacgttct ccgggccagc ccggacggcc
     3721 tcttcttcct catcggtgac gtgtccggga agggtgtgtc cgcctcgatc ctcatggctc
     3781 agcttcatgc catcttccgt agcctgactt cgaaccactt gcccgttgac gagctcgtcg
     3841 cgcgcgcaaa ccggatcttc tgtgaaagca cgctggccgc ctactatgct acgctcgtct
     3901 gcggcagggt ggatcgttcc gggcgccttg aactctgcaa cgcgggccac tgcccgccgc
     3961 tcctggtgcg cgggtctgag gtcacgaaga tcgaagccac cgggctcccg gtcggccttt
     4021 tctgcagcgg ccactactct gccaagggcc tgcagctttc cgccggcgac atgctgttcc
     4081 tgtacaccga tggcctctcg gaagcacgaa atcgcgccga caccgagtac ggtgaagaga
     4141 gactcgccga attgctgtgt gcccgtccgg gactttcacc ccacgaagtc acctccgagt
     4201 gccttaagga tctggcgaag cacctcggga ccgcgccgaa aacggacgac ctcacgatca
     4261 tggcccttcg ctacagcggc aaggcttgac taccgtcccg tctgacaccg cgccggggat
     4321 tcaactccga aatccgtaac ggaatccgtt ctccgcgatg tgccgcagtt cgtactccgc
     4381 aaagccaagc tcccgtgcgg caatctcata ctcacgtgtc agtgttgtgt gaaacatcgc
     4441 cggatcgtcg gtgttcagag taatcggtac acccgcgtcg tacagccgcc gcaccggatg
     4501 ctgtcgcagt gaaccgacgg atccggtgca cacgttgctc gagatggaaa cctccagcgg
     4561 gatgttgtgg tcgcgcagat gcttcatcag taccgggtcc tcgatcgatc tgatgccgtg
     4621 tccgattcgc tccgccccca gttccagtgc ggcccaaacg gaatccggcc cacccgtctc
     4681 cccggcatgc gccgtcaggt gcaggccggc ctttctcgct attccgaacg cttctccgaa
     4741 cagccgcgcc ggcccgcggt cctcgtcccc gccgattccg aagccaacga cgccatctcc
     4801 ggtcctctca gcggcgagct ccgccacccg gttcacgtgt tccacgccga actgacgtac
     4861 tgcatcgaac agccaccaca ctgtcacccg cgaatccagc gcctccctcc tcatagcgtc
     4921 aaagataggc gcaatttcca gcccgcgcca gatcgcaatg ccgacggaga agatgatctc
     4981 cacgtactgc acgttctccg attccagccg ctccagcagc cttcgggtcg ccaacgcata
     5041 ttgttccggt gttccgagct gatcgactac ccacttgaag cttttgagga actcgaggaa
     5101 attcgtgtac tcgtagcgtt cccggatttg ttcaaggctc aacacaggcg cgagttccag
     5161 gagcgtctcc ggttcaatcg caccttccag atgcacgtgc agctccgctt tgggcaaagc
     5221 gtgatagaag ttgcccatcc ctgccataat agcgttcgat gaaacagtcc gaaatcgaag
     5281 cgattgttgg cggctatcat ggcgacccct tcgggattct cgggccgcac gccgatgcga
     5341 aaaacggaaa ctcagcccga tgggaagtgc gagcgttcct ccctcaagcg gctgaagctt
     5401 ccacattgat tggtggcgct gcatggccga tggaaaaact ccagcgcgac ggcttctatg
     5461 tcgccgtgct cgatgacgaa ccgaaatcct accggctgcg gctcaagttg tggaacggac
     5521 acaccgtcga gatcgaagat ccataccgtt tccccccgct gctcagcggg tatgaccttc
     5581 atcttcacgc cgaaggcacc cttcacgagg ccttccacac gcttggcgcg cacctcgttc
     5641 agtgcgatgg cgtcacaggc gttcgattcg cggtctgggc gcccaatgcc gaggtcgtct
     5701 cggtcgtcgg tgacttcaac gaatgggaca ctcgccgcca ccccatgcgt ctgcgcgaag
     5761 gcggcgtctg ggagatcttc attcctgaaa tcggcgtggg tcgagcttac aagtatttcg
     5821 tccggtcacg agtccacggc caccggcaga tgaaggccga tccgtatgcg tttcagttcg
     5881 agacgccgcc aaagtccgcg tcgatcgtct cgaatctgga ctcctacgac tggggcgatt
     5941 cggcctggat ggatgcccgc gcacaacgcg atctgctcaa ggcacccgtc tccatctacg
     6001 aggtccacct ggaatcctgg ctgcgcggtc cccataacaa aatcctcagc taccgcgagc
     6061 tcgccgataa gctcgtcgca tacgtcaagc aactgggcta cactcacatc gaactgctgc
     6121 ccattatgga gcatcctttt gccggctcct ggggttatca ggtgattggc ttcttcgctc
     6181 ccacctcccg cttcggcccg cccgaggact tcatgtactt cgtcgaccgc tgccaccagg
     6241 ccggaatcgg ggtcatcgtc gactgggtgc cagcccactt cccaaaggac gctcacggcc
     6301 tggcgtggtt cgacggaacc gccctgtacg aacacgcgga cccccgaaaa ggcgaacagc
     6361 gcgactgggg taccctgatc ttcaactatg gccgcaacga ggtccgcggc ttcctgatct
     6421 cgagcgccct cttctggctg aagcgctatc acatcgatgg gcttcgcgtg gatgcggtcg
     6481 cgtccatgct ctacctcgac tattcgcgga aacctggcga gtgggtcccg aaccagttcg
     6541 gaggcaacga gaatctggaa gcgatcgact ttctccgcaa gttcaacgaa ctcacccacc
     6601 aggtccccgg ggccatcact gtcgccgagg aatccacggc gttctcgggc gtctcgcggc
     6661 ccgtttatct gggcgggctg ggtttcacca tgaagtggaa catgggatgg atgcacgaca
     6721 tgctgaagta cttctccacc gatccggtct accgccggca ccatcaccag aacatcacct
     6781 tcagcatgct ctacgccttc acagagaatt tcgtgctgcc ggtttcccac gacgaagtgg
     6841 tccacaccaa acgatctctg ctcagcaaaa tgcccggcga cgaatggcag cgcttcgcca
     6901 acactcgcgc ctttctgtct tacatgtacg cgcatcccgg caagaagctc ctcttcatgg
     6961 gttccgagat cggccagtat gaggaatgga accacgacgc atcgattcgc tgggaactac
     7021 tggagttcga ttatcaccgg aaactccagg cgctcgtccg cgaactgaac cggatttatc
     7081 gttccgaacc cgcactctac gaagtggact tccagtggaa cggcttcgaa tggatcgact
     7141 tgagcgacgt ggacaactcc atcatctcgt tcctccggcg cgccgccgat ccggaagatc
     7201 ttctgctgtt tacgtgcaac ttcactccgg tgccccgaga gggctaccgc ctgggcgtcc
     7261 ctgccgccgg ctattaccgg gaggtgttca acagtgactc ggagttgttc ggaggcagca
     7321 acatgggcaa cgctggcggt gtcaacgccc gccccatccc ttgccacggc cgccaggaca
     7381 gcatccagat caccctgccg cccctcgctg tcgtcgcctt caaacgtaga atgtagggct
     7441 gtagtatcgc ctgggatata cgcatgcaag ctggctttga cgtccttatc atcggtggcg
     7501 ggcacaacgg tctcgtggcg gcttcttatc tcgcccgtgc ggggcgtaga gttctcgtcc
     7561 tcgaacgccg tgaaatgctc ggcggatgct cggtcaccga ggaggtctgg tttggctacc
     7621 gcgtctccac cgccgcctac ctcaccagcc tgctcgagga acgggtaatc cgtgaccttg
     7681 aactcgagaa gttcggctac caggtcgacg ccaaggaccc cgccttcttc tccgcctttc
     7741 ccgacgggcg tcacttcttc atgtggcagg atcgccggcg gaccctcgat gagatctcga
     7801 agttctcccg ccgcgacgcc gaacgttacc ccgcctatga agaccacatc gagcgcctct
     7861 cccgcatcgt cgcccccctt cttctcacca cgccgccgca gttccctccc catggcgcgg
     7921 ccgagttcat cgactatctg aagctcgctg cgaagctcta cggactcgcc agacgcgacc
     7981 tcattcccct ggtccggatc ttcacgcaaa gcgccgcgga cttcctcaac gagtggttcg
     8041 agagcgaaga actgaaggtt actctcgcca ccgacggcgt gatcggcgcc aatggcggtc
     8101 cccgctcgcc cggcaccgcg tatatcctgc tccaccactg catgggcggc gtcggcggca
     8161 agcgcggcct gtggggtttc gtccgcggcg gaatgggaat ggtcgccgaa tccatcgccg
     8221 cgtccgcctg ccgtttcgga gcgaccctgc gcaccagttc cggcgtcgag aagatcctcg
     8281 tgcgggacgg ccgtgcttgc ggcgttgttc tctcaggcgg cgaagagatt gctgcatcaa
     8341 ccgtcgtctc caacctcgac cccaagcgga cctttctcgg cctcatcgac gagcgcggcc
     8401 tcgatcccga gttcgccgcc gccatccgcg cgtttcgctg cgagggaacg tcattgaaga
     8461 tgaatcttgc cctcagcggt ctgcccgaat tccgcgcgct acccggcgcg cccggaccgc
     8521 agcaccgtgc aaccatgcac atctgcccgt ccatcgacta cgtcgaacgt gcctgggacg
     8581 atgccaagta cggccgtcct tccgagtggc ccatgctcga gctgacgatc ccgaccatgt
     8641 acgacgcatc gctcgccccg cccggcaggc acatcatggg cgtttttctt cagtacgcgc
     8701 cctacactct ccgcgatggc aattgggacc aacttcgcga atcctacgcg gaccgcgtca
     8761 tcgacctgat cgccgactac gccccaaaca tccgcgacat catcgtcgac cggcagactc
     8821 tgacgccact cgaccttgaa cgccggttcg gcatcaccgg cggtaacatc tttcacggcg
     8881 agatgagcct cgaccaaatg ttcgtcatgc gtcccgtcgc aggctgggcg cgctaccgga
     8941 cgccgattcg cggcctttat ctgtgcggct ccggcgccca ccccggcggc ggcgtcatgg
     9001 gtgccccggg gtacaacgca gcgcgcgaga tcctgaaaga cacgtagtcg gagagcctgc
     9061 tttcagaaaa ttacacaagt cttacactcc acaagggaaa cccctgttag tctgaaaaag
     9121 tacggctgta tctgggagat ctcgtgacta ctgatgtgat tcgtatgccc cacgaccggg
     9181 ctctgaactg gcccactacg accgctttcg cgctcttcca cttgggggct gtcgcggcgc
     9241 tattcacctt cagttggact tctctcgccg ctgcggtctt tctgtactgg atgacgacag
     9301 gtctcggtat cagcatgggc taccatcgcc tgcacaccca ccgctcgtac cgcgtacccg
     9361 tgtttctcga acgattcttt gccgtgtgcg gcgccttgac gcttgagggc ggtccgatct
     9421 cctgggttgc gatccatcgg atccaccatc agaagtccga tcagcccggc gatccgcatt
     9481 ccccgcgcga tggcgcgtgg tgggcgcata tgggctggat tctgtttgga gaaaccaacc
     9541 acaacaacac ccggggcatg tcgaagtatg cgccagatct ggcaaagcac cgtttctatg
     9601 tctggctgaa caactaccac tgggttccga cggtcgtgct cgggctcctg ttgctcgctt
     9661 tcgggggtct ttccctgatg ctgtgggccg tttgcctccg tgtggtcttc ggcctccacg
     9721 ccacctggct gatcaactcc gccactcaca tgtggggcgc acgccgcttc gccactcgcg
     9781 acgattcccg caacaactgg tgggtcgccc tcatcacctt cggcgaaggc tggcacaaca
     9841 accaccacgc gcacccgacc tccgcacgac acggcttggc ctggtacgaa ttcgacccat
     9901 cttggatcca gatccggctg ctcagcctgc tcggaatcgc taagtccgtc cgcgtcgcac
     9961 gagttaccga ttccactgcc ggccgccaag ccgcataata gtccgccttg gatgcgccgg
    10021 actccttatc cggcataagc tgaagtcatg gtaaccgccg gaaggcgaaa atccatcgca
    10081 tttgtaatct cgcttggcgc cggactggtc ctggtcattc tgctcctcta tatcggctgg
    10141 gtactgctcg gctggcgcac cggcatcctc ctctttttcg gcgtactgat gctggccatg
    10201 gttatcagcg gaatcgtcat aaacaccata ttccttgtcc gtgagatccg gcgaaacgaa
    10261 cagcacaacg ccttcatcaa cgctgttacc cacgagctca aaacccctgt ggcctccatc
    10321 cggctgtacc tccagacact ccagaaacac gaggttgccg agctcaagcg gaacgagttc
    10381 taccgcatca tgctggatga cagcgaccgc ctcctggcca ccatcgaaca gatcctccgc
    10441 accggacgtc tggcttccac ccggcacgga ctcaacgttg caccgatcga tctcggaggc
    10501 gttgtggaag aatgccttgc ccgggccagg actctccaca acgcgtccgc tcaatcattg
    10561 cgatacagcc gtggtcccca ggtcacggtt ctcggagatc ccgacgacgt gcaggccgcc
    10621 gtctccaacc tcatcgataa cgctgtcaag tattccggaa gtgatgtgga agtgaccgtc
    10681 gagccgcccg aaaccggcgg gaagtacgtg tcggtgcgcg tgaaagacca cggacccggc
    10741 attcccaagt ccgaactgaa ccgcatcttt agacgcttct accgcattcc cgggccaatt
    10801 gccgcccgtg tcaagggaac cgggctcggg cttttcatcg tccggtccgt cgccaagcgg
    10861 catggcggcc gcgtctgggc cgaaagtgag gggccggggc gaggcagcac cttcgtgctg
    10921 caattcccgg tttcgaaatg agcaggatac tcgtggtcga ggacgagcag caccttgccg
    10981 aagggcttcg cttcaacctg gaggccgaag gctatcaggt ggatgtggtc gagacaggag
    11041 aagccgcgct cgacctgttg gtcgaacatc cggccgcctt cgacgtcgta gtgcttgacg
    11101 tcatgctgcc cggcgtggac gggttcaccg tgatgtcaga gatgcggcgt tccggtcagt
    11161 ttgttcccac actcatgctc accgcgcgag gtcatcccga ggatgtcttg cgtggctttg
    11221 ccgccggggc ggacgactat cttcctaaac cgttcgaact ggtgattctg atcgcgcgca
    11281 tccatggctt gctgcgtcgc cgcgagtggc tgcgtgcctc ccagcacagc gatacgtcat
    11341 cgtttgggtc ccgggatacc tacgcgttcg ccggcaagtc cgtcgatttc gaccgcctcg
    11401 aattaaacgt acgcggacag gtgttccagt taacgctgat ggaagcaaac gttctacgct
    11461 acttgaccag ccacgagggc acgccggtat cccgcaagaa gatgctcgaa gaggtttggg
    11521 gtctccacgt cgacaccgac acccgcgcca tcgacaattt catcgtccgc ctccgccgct
    11581 acatcgagga cgacccgacc cgtccgcgcc atctcctcac cgttcgcggc atcggctatc
    11641 gcttcatcgc ctccccaaaa tagcgactcc cggggaaacc ggagcctctg tggctacaga
    11701 ttcgcgtcgg tttcgaggct tcgccacaga taccagcttg ccattgtgca gtacggacgc
    11761 cacttccgcg ccatctcctc catttcagcc ggcgacggta gcgtctcgag cgcgtaagct
    11821 ctccgtatgg ccgctcgaag cccaagatcc cccaccggca ttacgtcagg gcgccgcagg
    11881 gcgaagatca ggaacatgtg gaccgtccac acaccgattc ctttgacttt cgtgaggctc
    11941 tccattacct cttggtcgga cagtcgctcc atcgccggga agtcgatttc cccagtcagt
    12001 accctctccg ccaagtcgcg gatgtaggcg atcttctggc gcgacagacc gagttcacgc
    12061 aacttggccg gctcgagcgt gagcactgcc ttgggcgtca cccgtccgtc gcctgcagcc
    12121 gccgccagcc ggccgaaaat cgtggccgcc acttttccgc taagctgctg atacacaata
    12181 gcctttgcca aagcctcgaa gcccggctcg atgaaccgga tgcggcatgg cccgatctcc
    12241 ccgatcaggc gagccagcac gggatcggct cgcctcaaat ggtccagcgc cttacgcatc
    12301 ggtaacacaa tcagcggcgg atgcatccaa cagtatgtca ttgtaccggt atgaggcccc
    12361 tcaatctgtt cgccgtcgcc gtcgccgcga tgctcatcgc gccggcccag gaacctcagg
    12421 aaacccccac gatcaaggtg gaagtcgacg ttgttaacat cctctgttcc gtccgcgaca
    12481 agcgtggcag cctcatttcc accctcggca aagatgattt cgtcgtcaag gaagatggca
    12541 aggcccagga catccgttac ttcgcccgtg aaaccgacct tccgctcacc ctcggacttc
    12601 ttgttgatgt gagcaagagc caggagcacc tgatcgagat cgagcgccgc gccgcggcgc
    12661 agttcttttc ccaagtgctt cgcaagaaag acatggcgtt cctcattagc ttcggccccg
    12721 aggtcgagct tttgcaggac tacacaaatt cccaaacgct cctgcgtaac ggtctcgacg
    12781 agctccacgt gcgaggagcc gtcggcggac ctctgccggg cccggtaccc acaatctcgc
    12841 aacctcgggg aaccgtgctc cacgatgctg tatatctggc gtcgaacgag aaattgcgcg
    12901 gtgaggtcgg ccgtaaagcg attgtgctca tcacggacgg catcgacacc ggcagccggg
    12961 tgaaactcga acaggcggtc gaggccgctc agaaagccga tgcgatcgtt tacagcattt
    13021 actattacga cccttcggcc tactaccagg gcggtttctc cttcggcggc gggaacgact
    13081 cggcccttcg caagatgtcc gaggagaccg gaggccgcgt cttcaaagtg agcaagaaga
    13141 acacgctcga cgacatcttc aagcagattc aggaggagat gcgaagccag tactcgatcg
    13201 gttactcgcc cgcgaatccc gcaaaggacg gttcgttccg acgactcgag gtggacaccg
    13261 cccggaaaga cctgaaggtc caggcgcgca agggatacta cgcggtcaag gccggctctt
    13321 agtgagcaag ccctcgcgcc gattcaggta gtccgtgacc gcttccgata gcggtggcat
    13381 cggtgcaatc ccggccgctt ctacctttgc gttggagagc gctgagtact tcggccggcg
    13441 cgcggccgtg cggtactcgc gctcattggt cgcatgaagc ctcggcgtaa gcccggcggc
    13501 ctgaaagatc accttcgcat attcgaacca tgaggtcgcc atgccgcccc cgatgtgaaa
    13561 cagccccgag aggttcttct ccacaaggtc ggccgtcctc gacgcaagtg cgggcgcgta
    13621 cgtcggtgac gcgaaatgat cctccactac gcggatcggc tggccggttg tcacgatgcg
    13681 gagcattacc tcgacgaaat tcccccgtgc cgtcgccagc ccgccgggcc caaatacacc
    13741 cgacgtccgg atcagtaacg gacgctcgag gtatgcccgc gcgaagaact ccccggccag
    13801 tttggaaacg ccatacgcgt taagcggccg cggactatcc tcttcggtat aaggacgccc
    13861 cagggtgccg tcgaaaacgt agtccgtcga gtagtgcacc agccgggcgc ccacttgccg
    13921 gcatgctacc gcgatgttct gcaccgccaa tgcgttcacc gcgaacgcag gcgccggctc
    13981 cttctccgcg acgtccactt ggttgtaagc ggcggcgttg atcaccacgg cgggatcgaa
    14041 ctgcgcgacg cagtcctcga cacgcgatgc ttccgtgatg tctacctctc cccggtcata
    14101 ccccgcgacc cggtagcccc ggttcgtgaa ctcccgcacc agttcgacgc cgagctgtcc
    14161 ggcatttccg aagatcagta ctttcctggg catcaaacgg ctaggatagc gcgaaaccag
    14221 cctgaaatcg cctacttctt cgtgccgaat gtgatgttca cggtggtctc ccctgaccca
    14281 gggttcaaga tcaccgcgac agtgcggccc tttgcttcgt ctgtgcctgt cactgacccg
    14341 ccctcgccgg attcgttggt ggtcacgttc atgccgcctt gcttgaaagc gtccttgtaa
    14401 aatgcgatca ccgtaacagc cgcatccccg gtggtgaaat gaaatgtgcc gccctcgccc
    14461 tcagtcccct tcatcacgaa tctgccctcc gccttcgccc cgggataagg aggaatccag
    14521 tctggcaatt tgtcggagcc ggtcccgaat tccatcgtcg ccgtcccgcc cttgccccct
    14581 tcctccttga aaacgatctt cccgcgcttg acgtcctcaa agttcatcgt gaccgttttt
    14641 ccggtgctct tttccttgag cgtaatgaga cccttgccct cgtcaacgct gaccacttcc
    14701 acgtccgggt tcgtggcgac gatcatcttg ctaatcgcaa gcgtggggtt gctcctcatc
    14761 agttcgggat cgactccggc ctgtttggct ttgtgataaa cgaagagccc accacccacc
    14821 accgccagcg ttcccagcac gaacagcccg agcacaacga ttaagaccca cccgagcggt
    14881 cccatcctgc gcttcgccgg tacttggcct cgcgccgccg cttggatgtc tgtcgccgct
    14941 ccggtcgacg gtgttccgcc gctgaccgga gtgccgcact gagagcaaaa cgtgccactg
    15001 acggaagcgc cgcaattggt gcagaaagcc atacatcccc ccttcggcaa ctctacgcca
    15061 ttcgcgtttg gctggcaaca gttctctcgg ctgcgggccc gtttgactct tccatcagcc
    15121 gctgcatcag tcgagagagt tcttcagaga tggtcagcgc ctgctcgagc tgaagcccca
    15181 ccgcgtacag gtcgccgtcc gtgcggcaat acgccacttc acccaagagc aggctgccgt
    15241 cgatgtcaac ccggaccgcc gctccgaccg gcactcgcgc acgaatccga agacacatcc
    15301 ccctgccgga gacgttgaca atcttgccgt ccgtttcgag cttcgctccg ctcagaacgg
    15361 tgacccgcac aggctcgtct gctggaaatc gtggttcaga cctgaggtcc atcgcccata
    15421 ggatcggcga tttcggacgg aaaccttaga gaaggcgaga gagcaccagc cagatcgtga
    15481 tcgccgctgc cgccgctgtc gtcgccgcca gccagtggcg ccttcggtaa acccaatcga
    15541 ttgcccggct tacaaaggtc tggtccggct cctcgctgcc gttcaggatc cgtaaggcga
    15601 cggctgaatg ctggccccgc aacgctcgga ccacttcctc cctacctccg cggtccgcaa
    15661 tcacaatggt tgctgactcc cattctccgg gtctgtcgat gagctttcgc cgcacatccg
    15721 ccgggacgtc gtcaatcgaa gggtatacct cagtcgtctc ccctaccgag acgaaaaccg
    15781 tcgattttgt cagcaggcgg cgcctccggt tcatcgcgat attaccccgc aggctgcgtg
    15841 ctcgagcact gagtctatga aaccgagtgc ggctcgcgac agcgccttgt cctttcggta
    15901 gatcagcccg acatgccgga tcatcggctc aggggccagc gatatcgccg ccagtttccc
    15961 ggaatccact tcttcccggc agtgtgacgc cgccatgaaa gacaccccca ggccggcaat
    16021 cacaaaccgc ttgatcattt ccgtcgagtc gagctccatc gtgatgtgga tgatgtcctc
    16081 gaccagttcg agccatgcat caagccgcga cctcgtattc cccgtcttgg gcaggattag
    16141 cgaacagccc gcgagatcct ggcagctcac gtattctttt cgcgccagcg ggtgaccggc
    16201 aagactgatc agcttgatct cgtctgagtg gatcttcacc acttgcagct tccgatcctg
    16261 caccggcagt tgcgtgaacc cgaaatccgc ctggttctcc atcaccgctt cgaccacccg
    16321 ttttccgtac gagcgatcca ccatcagttg cacgttcggg aaccgcttcg tgtactccga
    16381 gaagacctgc ggcaagacat agatacaggt cgcctcgttg gctgcgatga caagcgcccc
    16441 acgcggaacc ctgtccagct cattgatggc gtcctgcgcc cgccggcgca gatcgaggat
    16501 ctgctcggca tactcgcgga aaatccggcc ggccggcgtg agcgcgatcc ggctcccgag
    16561 ccggtcgaaa agcgacgtgt ttagctcacc ttcgagttga cgaatctggg cgctgatggc
    16621 gggctgcgtc cgaaaacagg tctcggcggc tttcgagaaa ctcttcagcc tgacgatctc
    16681 gagaaaggtg tgcagttgat cgagatccat gcgagcccaa ctcgattgta ccaattgagg
    16741 ctcgccggac catctacctg gcggcggctg tgtcgaatca gcccgagcgt ctgtctgcct
    16801 gcctttgtca attgcccagc agccaggaaa cggtgcatcg catcagcggc agggctttgt
    16861 cggccctgtt cgcaaacgcg cccgagtcgt gagtgcagct cgggttttgg gccgcggcca
    16921 cgtaagcgag cgagacgaca aactcaggcg cgaacgcgta gtagaccacc cgtcccctgc
    16981 cggcgcgggt cgaaaggatg gccggcgtgg gccggctcca atccgtagtc tcgaggaact
    17041 gcacatatcc gagcactccc ggatagttct ctggacgttg gaccaggatg ttggcctcgg
    17101 cgccgtaggg cagcacgttg tccaacggcg agagcgtcga ttccacgtct gagcagccgg
    17161 tcggacccgc aaactccgta acccggatgt ccacgccgga ggcgaggttg aagctgctaa
    17221 acaccgtcgt catcggatca tgccaaagct cgtggagccc gaaggtgtgc cctttctgcc
    17281 gacggagccc gtccgcctca cggggatcga ggcgtgtcga tttgtagccg cttccaaacg
    17341 tcgcaagcag ccggccaccc cgcaccaccc actgatggat tacctcggtc tgttgcttgt
    17401 tgaggctcgc cgcgttggag aggattagga ccttgaacag ggctaggccg tcttctgtga
    17461 cgtcagcgtc gtggatgacc gtaaacggta tgtcgggtga ctggctgagc gcgtattgcc
    17521 agcccatgaa gtaccttgtg tattcctccc catccggcag gtaaaagggg cagccgttat
    17581 agtggagtct gaaaaactcc tttgtgttag cggaatcaag cagagccact tgaggctgtg
    17641 cctgaagaag cgtagccagc aggatagccg cgattgccag acgccccaga gtcttcatat
    17701 aatcacctcg cctttcctaa aactcgaaaa acaagcccgt ggacaagcct gcgcgacgat
    17761 acgcggtgag cttgtcctga tagtcgaacg aaacgacgaa acccacgtgg cgggagagcc
    17821 atttccggta gaacgtattg atggaataac tcaacaaccg aatctcagcc ggtgtgatgt
    17881 cggtgaaacg gtactgttcg cggcctccac cagcggtgac gccgaaccag tagtttcctt
    17941 ccgtcccgta ttgggctgca agagtgccgg cggccgagac gaagtcgccc ggttggttcc
    18001 ggttgacgaa caggttgcct tccataacga gttttttgtg atagtagagc gcgccggcat
    18061 tgaagatctg cccatggcct ggaccgccat agtcgaagcg ggtgaaaccg gcgccgagga
    18121 cgaaattccg cgtgggtggc accttccagt aagctttaat gtcgtagcgc cgtttgggaa
    18181 aaaagattga ccgttcgttc cgctgcgggg cggcgctgaa accctgcacg gtataaaagg
    18241 acttgctcca gttgaggtaa gacaagaaac cgtagttctg ctgggtacct tcgggccgcg
    18301 tctgggtgtc aaaaatcaaa gccgggacaa agcggggatt actgcgaatc cacaattggc
    18361 cctgaagccc gcgccaagtg ccgagattcc ggttcagttg gcttccgtac gccccaagtt
    18421 cgaggcgcac gggcagcacg tcagactgtc cggcgttgcc ggcgtcctgc gcgaagcaac
    18481 cggctacacc aaacagagcc aggcagactc gaccggctga attcaatagc accgaagctc
    18541 ctttcagacg cgtagcaacc gtgacgtctg aattttcctc cgcttccgcc agtcctcagc
    18601 tccggcagca tccaccacca tcacgccgcc gggggaagcc aatttcccac agacaaccac
    18661 gttgttttcc acccaaatcc cgtcccccgc gtaagctacc accggtgcct ccttgccggc
    18721 gccacgcgtc ccctcgctga tcagtagcga tccgaaagcg tggacaaccg cggcaaagcg
    18781 gcagtcaggg gccaggtaaa tatctccgcc tgctacaagg ttgccttcgc agaacccgcc
    18841 tcggccgatg tgtaacgatc tatccgactt gaggtcgtgt cgcaccgcgc tgcgcggtgg
    18901 gagatggcag cgccccttta cgatcagctt cgcacccacc cggagaggcc ggcttggtgt
    18961 gagatctccc gcgtagtgcc aactatcggc actcatgcgc acgaccttct tcgggtctac
    19021 gcccgcatca agcaacgctt gctccgccgt cgacgactcg tccggaggcg gaatctccac
    19081 gagatcatcg ggcggaggca ggtcctgact cgacggggaa ccgttcgaac cgggcgtgct
    19141 gacgcgagga gcaaagactg acaaaacccg gcaacccata ccaagtcgca ctgccgtccg
    19201 tgacgtcacc cgcgctccca cgcggcaatc cgtcccgagg atcatttcac cggtgctgtc
    19261 cagccaccgg gcggccgtta caccgtcttc cagaaccgcg ttaccgtccg ccgccaaaga
    19321 ttgcagccgt gagccatgcc cgatccgtgc actgccacgg gtgtaaatct cgcggaagaa
    19381 ctcgcagcct gacccgcagg aaaaatctcc gtctaccacc cagatcgcat cggactcgtg
    19441 tcgatcccca atggccattg gaccggtggc gcgaattcgt tcatggccct tgagaatagt
    19501 gcgctccgtg ccggtcacgg ttagcgccgg cagttgcagc cattccttga ctttcaatcg
    19561 gaaagactgg gggaggcagt cctccatcct cacgtaggtg acgtcgatct caagggaggg
    19621 ccttcccttc agcctcctcc attggcggtg tgccaggtag aaatgcaagt acagaatcag
    19681 tgcaagtacg ccggcgaaga tgaggaagcg ccaagggatc actgttctga ctcagtgctg
    19741 aatgtatatg tgtaagcgcc actcggtcgc tggctgaaca agccgcggcc ggatccgttg
    19801 ccattccgaa actgttcgcc gttgctgttc tcgcggaaac gcttggtctt gtgccagcgc
    19861 ggtccccggg atccccacac gcggcctacg tagtaacgta gcagggcgcc gcagattgcg
    19921 gctgtactgg caaagaagtt gaacagattt accgggatga gcaggacgcg ccgggcgtcg
    19981 ccatccagca gggcggcggc gccgagttcg aaaaacgtag cctggttgcc gaagacctgg
    20041 tagcccgcga aggccagtgc aagaagcagg ggaggcacgg ccgatgcctc cgggctgaag
    20101 aacaatacca acgacgccag ccagcccagc accagaatcg gcgcggtaag atagcagccc
    20161 aggacgaaga gcgcgtccac tttttccgga cgggacagaa acttcgatcc ggagaccgaa
    20221 gaccacaacc ggtgcaggca gtccgtgtgg ccggtcgccc agcgcgctat ttggcgtttc
    20281 ctgacctccc acgcctcggg aacctcctcg taacactcgg cgcgattgac gtaggcgacc
    20341 ttccatcctt tcgcaatcag ccggaacgtc aggtccgtgt cctcggtgag cgatttcgta
    20401 ttccagccgc caacagcacg cagcgccgac gctcgcacgc cgccgaccgt ccctccgaac
    20461 tgcggagtca agccgagatt gtagcgggcc tgctgcccga cctggtaacc ggcagcgcgt
    20521 tccagcccca gcagtgcggc gagcagcgtc gacccggagt tgtggggaac cacgcggccc
    20581 atcactgccc caacttcggg atcacagaag ggggcggtca actgcttcac catcgcgcgt
    20641 cccggaaagt aatcggcgtc gaacaacaac agaacctctc cggacgcctg ccgcgtcgca
    20701 agttcgaggg cggcggcttt gccgccttct ccgtcgcggc gatgaatggc tttgatgacc
    20761 gggtatcgcg cggcgaaatc gctgatgatt tctcccgtcc ggtcctccga gcggtcgtca
    20821 atcgccagaa tctcgatccg gtcccggtcg taatcgcctt ccaccaacgc ttgaagcaag
    20881 tcggccgcca ctttctcctc gttgtgcatc ggcacgagca ccgaagtacg ggggaggaag
    20941 tagccggtta tctccatcat gtcgcggggg cggcgtatcg agaggcggta tagggcgagc
    21001 gtgtagtgac gcaacccgta aagcgccagc acggcgatgg caaacagcaa acaccctttc
    21061 gcgacaagga tcaggatggt catagcgcct tcctccgcca atagcgggaa gcgttccagt
    21121 ggacggccat cgagtagaag accatcaggt agatcatgtc ggcgaggaca ccgagagcga
    21181 accagagtat cggaacgaaa aggatcccgg aataatgcat gaccgcgaga cagaaggcca
    21241 tccggaccgc cgaccaaaat attccaaaga cctgcaccag caccgtaagc gtcacgccga
    21301 tgagccaaat tggctggacc agcgtgaaca tcgctgcgga gaagacaggt aacagtaacc
    21361 agacaagcac ttcgccgcgc agccagatct ggccgaattc gactgccccg aactggctcg
    21421 aagggtgaat ggccaccacg caggccgtaa ttagcagcag ggccgcgagg aaaagcacga
    21481 atccgcggga gagcgcgatg cggtgctgga tcgcccacag tgcgagcaga ccagccacgg
    21541 ccagcagcac gaagcgcaag ggattcgact cgaatcgggc gaccggcacg gcaggcacca
    21601 cggctgttga gagaccctca aaaagctcaa tcgtttgctc cccggtcacg gggacgccgg
    21661 tccactccag cagcgtcaca cagaggcggt cgtgccatgt gaagaattca gcgttgtacg
    21721 cagcgatcga accgcacagg acagcagcgg tgaacaccgc cgagagcagc tttgcgaccg
    21781 taaccctgaa cgatcgcaca ctgcggtgcg ggtagataaa ctcccggcgc atatggctgt
    21841 gcggagtagc caggaaggcc ggcatccgaa ccttgagcga gaccgtcaca cgattcctta
    21901 tagtctatgc caatcgtaga tgggttgccc caggcacaac atccacctaa cggtgttcga
    21961 gtactagtaa gggtcaaccc cacggtacca gaacctcgac cgccggccca ctatcgagca
    22021 tcgtgatgcc ggggcgtgga taattgaagc atgccgcgaa acaagcttgc gctgatcgca
    22081 ttggcgcccg cggtgatctg gtttaccgca tgcgggccgg acaaaaagga ggagccgaaa
    22141 gtggtgaaag cgccggagca gttccgggtg aagtttgaaa caaccaaggg agacttcgtc
    22201 gtggaggtga cccgcgcatg ggcgccacgc ggcgccgacc ggttttacga gctggtgact
    22261 gagcgctact acgacggttg caagttcttt cgcgttgtgc cgcgcttcgt ggtgcaattc
    22321 ggactaaacg gcaacccggc gttgtcccag aagtggagcc agatgacgat cccggacgat
    22381 ccggtgaaag cgcacaacga aaaaggcacg ctcagtttcg cgatggcagg cccggccacg
    22441 cggacgactc agatcttcat caacctcaac gacaacattc atctcgatcg gtcgaacttt
    22501 gcgcccttcg ggaaagtcac ctcgggaatg gaaatcgtcg agagcctctt caagggatat
    22561 ggcgacgcgc cgccgcaagg cgtcggcccg gtgcaggcgc tcatccgcga agagggaaat
    22621 acttacctcg agcgtcactt cccccggctc gatggaatca agacagcgac tgttgagcca
    22681 gtcgagggcg gcgcctgaca gggttggttg gcagcctaga gtaggactcc tctccagcgc
    22741 aacggcacag gcttcagcca ggcttaagcc tgtggcgtgg gcttttcatc gccccgctaa
    22801 ggcggcttct ccggccagga gtggcgcggg tacttgcggc tgagttcacg gcgcacctgt
    22861 gggtaaagcc ggtcccagaa acctgccagg tcggctgtca tctgcacggg ccggtggttc
    22921 ggggcgagta gatgaaccac gagcggcacc ttgcccccag ccacccgtgg tgtctcgcgc
    22981 agcccgaaga aatcctggag ccgcgaagcg atccacggcg gctgcccacg ggcgtagcgg
    23041 accgggacct gtcttccgcc cggcagccgg atccgttccg gtgcagcctc ccgcagcaat
    23101 cgccgggcat ccggcggcag ccggcgttcg agtgcgcgca gcagaccgcc gcgcgcaacc
    23161 tgctcgagtt cggtgaaact gcgcaggccg tcgcagagcg attcgaacgc cgccttcacg
    23221 tcctcatcac cgagagacgg aatccccgta tgctcagagg caaaggcaac tcgagcaaga
    23281 aaaccttcga cctcctgcgc atcggcgaac cgccccacac ccgcctccaa cgccttcctg
    23341 gcgaggagct ccgccgccgt ggccggctcc ggctgcgcat tgcggctctc ctcgatcacc
    23401 aggctgcaga agaccagcgc gctgacggcg tcaacccgtt ctgcgtttct gttccattcc
    23461 acggcattgc gctcggagac ctcgtccgga aacaggtcga gcagccactc cggttcgatt
    23521 gcgctcgcca ggcgtacgag cgggagcccg cgctcgcgtc gctcctcgat gtccactgcc
    23581 accatccagt cgcgcgcacg caccacgctc aacggcgaga gcaccgccga gcctcccgcc
    23641 gccagcagca gttcgtcgcc ttgacgccgc ctcgcaacgc ggtcgggaaa tgcggcgagc
    23701 acggaagcga ggagcgccgt ctcatcgtgc ttcttctgcc gctccgggcg cgctgagcgc
    23761 cggatctgct ggtagacccg ccgtgtgtat ggtccccatt cggagtcgag cagcaggagc
    23821 agatcgctcg ggccggaatg gtgcgtctcc agtggaagcc tctcaccggc actcaggagc
    23881 gcagccagca cgcagccgtc atcccccgac ccgcgccggt ccgcctccgc caccaagcgc
    23941 gcgagccttg gatgaagggg aaggcgcgac atcacacgtc cggcttcggt gagccgcctg
    24001 cccgcgtcga cggcaccgag ccgccgaagt aactgctcgg cagcctccac ggcagccgcg
    24061 ggtggcgcat cgagccattc gacgtccgcg agcgagcccg cacccatagc gcgcacttcg
    24121 agcgcggtct gcgagagttc gcggcgcctg atttccggag cgtcgtattc gggcctgcga
    24181 tgaaagtcct ccgcgggata aaggcggatc acccggccag gacctgtccg tcccgcacgg
    24241 cccgcgcgct gggttgccga cgctttgctg acgcgggcca cacgcagcgt aggtagcccg
    24301 gaccacgggg aatccgacgc gacgcgtgcc agaccgctgt cgatcaccgc tcgaacgcct
    24361 tcgatggtga ccgagctttc ggccacgttg gtcgacagga tcaccttgcg ccggtccgcg
    24421 ggcgcgatgg cgcggtcctg ctcctcgggc gagaggtcgc cgtgcagcgg aagcaggagc
    24481 agtccggccc gctcggcaat cggtccgcag gtacgcgcgg cttggcgtat ctcggcagcc
    24541 cctgggagaa agacgagaac gtcgcctttg agtccttcgc ggagaagaga ttcaagcgcc
    24601 gaagccacct gctccgcgag agtcgccgcg gaaaccggcg tgtacgagag cgcgagatcg
    24661 aacaggcggc cctcggcgcg caatacacga catctgccaa gaaaggccgc caccggtgcg
    24721 gtctcgagtg ttgccgacat gaccacgagg cgcaggtccg gacgcggtcc gcgttggagg
    24781 cgcctcagga gcgcgagggc gacgtcgccg tcgaggtgcc gctcgtggaa ctcgtcgagg
    24841 acgaccgtgt ccaccccgcg cagttcgggg tccgagagga gccgtcgcgc gagcacgcct
    24901 tcggtcagaa agcgcaggcg cgtgcgcggc cccgccactt cctcaaaccg gacctgatat
    24961 cccaccgtct ggccgacttt ctggcctgat tcgtacgcaa cccggcgcgc ggcgagccgg
    25021 gcggccaggc ggcggggctc gagcaccagc acgtcaccgc acccaagccc aagcagggcc
    25081 ggtggtacgc gcgtggtctt ccccgccccg ggaggcgctt cgagcacgag gctgggcgac
    25141 tctcgaaggc cggacagaat gtcagggatc agcgtatcga ctggcagcgg cgcccgcatc
    25201 ggttcaaggg aagaggttac gaaactatac taatctgtat gcctgatacg tacgctcccc
    25261 tgaatccacc taaacgccta ctgatgggcc ctggaccaac gaccgtcgag cctcgtgtgt
    25321 acgaggccat gggcaagcag atcgtcggcc acctcgaccc cttcttcttc gaagtggttg
    25381 aggacatccg caaactgctc ggcctggtct tcggcacgtc caacaccttc aacatggcca
    25441 tctccggcac cggcagcgct ggcatggagg cggcggtggc gaacttcgtc gagcctggct
    25501 ccaagttcgc ggtgttcgcg aacggcttct tctgcgacag gatcaccgag atgggacacc
    25561 gctacggcgc caacgtggtg cgtttcgaga agccgtgggg agagacattt gatgacggcg
    25621 aggcggccgg cttcatcgag cgggagcgcc cggccgtggt cgccttcgtc cacgccgaga
    25681 cttcgaccgg cgcgctgcag ccaggaaagg caatctgcga ggcggcgcac cgcgcgggcg
    25741 cgatcgtgat tgccgactgc gtcacgtcgc tgggcggcat gccggtgaag atcgacgaga
    25801 tgggcgtgga tgtcgcctac agttgttccc aaaaagggct gagttgccca ccgggacttg
    25861 cgccggtcac cgtgtccccc cgcgcgctgg aacgtttgcg ggcgcggact acgccgcagc
    25921 ggacatggta tttcgacttg aagctgctcg acgagtactt cgagggagcg caccgctatc
    25981 atcacacggc gccgatctcg atgttctacg ccctgcggga gggccttgcg atcatcgccg
    26041 aggagggcct ggaaaaccgc tgggaacgcc atcgccgcaa ccacctggcc ttcgtcgcgg
    26101 gcgttgaggc gatggggctg cggatgcacg tcgccgaaga ccgccgcctg tgggtgctca
    26161 acacgccgag ggtgccggac ggcgtcgagg atctcaaggt gcggaaacgg ctgatgggga
    26221 aacacggcat cgagatcctc ggcggcttcg gcccgctggc gggcaaggtg ttccgcgtcg
    26281 gcattatggg ggcctcatcg acgcgggaga acgtattgct gctcctcgat gcgctcgaag
    26341 aggcgctccg caccgagggc tatgaaccga aggcgggcgg gcgcgccgcg gcagagcaag
    26401 tctacgccgc cccggtgggg taaaatttcg ccatgcggcg cagaacttgc ctgaagacgc
    26461 tggcggccgc ggcaggggtg tcctccatat ctgccgccgc cccgcccatc cagttgcatg
    26521 tggatctcga cgtcgattcc gcgaaggaaa agaaactcgt cgagaatttc cggaacgtgt
    26581 tccgtcccgc catcagcagg cagcccgggt tcgtggacgt gaaactcctc agacttcgga
    26641 aggccctctc cggcccaacg cctctgccct accgcctgct aatcagcttc cagactgagg
    26701 aacagcgaca ggcctgggtt gccacgaccg atcaccagcg agcctggcca gccatcgaag
    26761 aaaacctgcg gggttccaag tatacggctg tgctgtacga cgcggattga gtctcgttcg
    26821 atcagcgact gggacacaag ggagcaatag cccggcgtca aaatcacttc acgcgccggt
    26881 attccccact cgagaacgcg ttttcttccc gaacgacccg tttcaaaaag cctcggattt
    26941 cctcggtgga tgcggtcaga actatgctcg gttcatcgtc aatccggacg agcgtgtgtc
    27001 cgatctgcac cctcttctcg cgtaacccat cggcgagcca ttcgggtttg agcacgccgg
    27061 cccgcagcgc gtcgccactg atctccaacc gtacgaacac atgagcaggg accgagaacg
    27121 cgtcgtccga tttcttcggt gtgatatcca gaaacaggat ctcctcgacc tttcccagac
    27181 gcccctccag ataggttgac ttgttgccat ccctgcaaat cacctcgtag gttttcggac
    27241 ctgaaccgat cacggtccag gtttcgtcgt cgccctctgc ctgccacgtt ccgagcagtc
    27301 gcggctctac tgccagatca ctcggcgtaa acaacggctg gagcgatcgc acgagacatc
    27361 cgggcgcgag aatgcagagc gccagcagca cgcacaacag catgacacgc atcggattcc
    27421 ctcctcggag aaagctagta cgcccaccgc cacagtccca cgccggaggt atatcccgcg
    27481 ccgaccgccg cgaacagcac caggtctcct ttcttgagcc tgccttcggc cagtgcgtcg
    27541 cgcgtcgcca gcgggatggt tccggcggtg gtatttccga atcgctggat gttgataacc
    27601 acctgttcgt cgctcaaacc gaggcgatcg gcggtggcgg tgataatccg gcggtttgcc
    27661 tggtgcggga tcattaggcg gatatcccgt gcgctgatgt tgttccgttc cagcagttgg
    27721 ttgcacaact cgtacatctt gcgaacggca tacttaaaga cctgctggcc ttcctgatgc
    27781 acgtaatgca ggcggttctc gaccgtttcc accgaggccg gctggcggct tccgcctgcc
    27841 ggcatcttca ggaaatcacc accggagccg tccacctcat tcaggaaatc gacgaagccc
    27901 tgatcctctc cgtccgtcgg ctctaccagc atcgccccgg cgccatcgcc aaacaggacg
    27961 cacgtggcgc ggtccgtgta gtcgatgatt cgggacatcg tgtcggcacc gatcaccagc
    28021 accttcttgt gggtgccagc agcgacgaag tgagcggccg tggtgagacc gaacaagaaa
    28081 ccggagcacg cggcgatcag atcgaatccc caagcgccct tggctccaat cctatcctgc
    28141 accaggcaag ccgtcgacgg gaagaacatg tcgggagtaa cggtgcagac gataattgca
    28201 tcgagttcgg cagcttcaat tccgcgctgc gcaagagctt ccctcgcggc ctctacggcc
    28261 aggtcggatg tcgccatccc cggttcggca atatgacgtt ccagaattcc ggtccgctcc
    28321 atgatccatt cgtggctggt ttcaaccagc ttctctaggt cggtattagt cagcaggcgc
    28381 ggtggggtat aacacccgag ggcgcttatc tttgccctac gcacgggctt ccccgaagtg
    28441 tttgaaaacg aagatgagtt cactctgata tggtacattc gggtgggatg caagtcgaga
    28501 gcgcgctact ctttgagacc ccggcggaaa tctgggcgag agtctaccgg gaactaaggc
    28561 cccgctcgcg gatgccggaa atcacgatcg agtttcgcca gtttgcaggc gcaaatacgt
    28621 ttgcccggct cgagggtggc ctactgcgtg tccgcatcac ggatgtgctt gagggagcgc
    28681 cggcccccgt gttggaagcg ctggcttaca tcctgctgtc caagctgttt cgccggcccg
    28741 ttccccgcat ctaccaccat cgctaccgaa tgtacctcaa ccggaaagag atgcgtcgca
    28801 gtctgcactt gctccggcag atccggggtc gcaagctcct ctccggaccg cagggcgacc
    28861 actacaactt ggagacggtt ttcgaggatc tcaacctccg gtacttccac ggcttgatgg
    28921 cgaggccgct cctgagttgg agccaccgtc cgtcgcgcag tatactcggc cacttcgatc
    28981 cgtcgcataa tgcgattgtc atcagccggt tgctggacag cgtctcgatc ccgaaaatcg
    29041 tcctcgcgta cgtggtcttt cacgagatgc ttcacctgcg ccacccggtg gagcacgctg
    29101 gagctcgccg gcgcgttcat acaagggcgt ttcgcgaagc cgagagggtc ttcggggggc
    29161 tgaaagaagc caaggaatgg cttaagaggc tttagtccct ttcacccgaa gggctcttcg
    29221 gacagaccga gcgatcggtc gccgagagac gccagccaat ctcgttgtta caggtgggat
    29281 aggacggtca ggccaccgag gcctgccggg ccagagccat ctgaccgagg tggactctca
    29341 ttacgtcgag cgcacccacc agttggcgaa tgtctactgt gccgattccc agccagtgta
    29401 acgccaggcg gacctcgacg gaccagatcg cgtatgtgaa atgcaaccgt tgcttcagca
    29461 gggccttcgc cagctcgggc tgatcctcgg ctgaatcgac cataaacagc cggacggcag
    29521 cctcgagccg ttggaagtct cgtcgaatcg aacgcagata ccccctgaag attcctctgc
    29581 gttcagagcg taaccgttta ccgatcttcg gttcgtagcc ctcctgcgag accaggaact
    29641 cgaagtcctc ctccgacagc aagcgctcca taggccggta tttcgaaacc gagaaattct
    29701 ccagccattc aggatcaaag tccgggacac ggtcgcggaa cagcatccgc ttgaaaatca
    29761 gaccgaaccc gactgctgtc gctaccacca gagctaacgc agccgcgatt gcgatgtcca
    29821 tttctcatgt tcctccctag aacacgcgat caggataact gagacatcac cagattgcaa
    29881 tagtatttct agtacgttta gtactatatg tgcaactaat tgaaaaaaga tcactttgcc
    29941 atgtcaaacc gttcgaggtt tcacctattg gcgggtgatt ttccggattc ttcgcggtgg
    30001 tccgttttgg aactactggt acttccgttg catctacatg aagaaaagct acttgcggga
    30061 agatcgtaag agtgccgcat tgatggcgtc aagttggcga acaagctgtt caccctcttc
    30121 ggggcgccat tgtcgtctca ccgaagcctt ttgctcctcg ccttgccggc ggaggaagag
    30181 gatccagata acgaggcatg cgcaggtaag cgcgatcagg atcgtgctga tgatgcgggt
    30241 gatctcgagc ccgcggatgt tctgaacaaa gagcgccagc gtagagcaca gaaaataaac
    30301 ggaatacacg atggcatgaa gaatcacgtt ccggccgaga gggagaggat accagacaac
    30361 gaagacggag atcacaagca gaaaaatcac caggctcgaa ttgatcccgc gctcgatgac
    30421 attgtagtag atcagtgtcc ggtatcggcc gccggcattt tgaaggtcag gcactaacgt
    30481 gacgagcgca atcagtaccg ccgccgccag cgcagccatc atcgtccagc gtcccaagct
    30541 ggcgattccc tggtagcggc gcaagactag ggaaaacaac tcgagcacga tcaggatcga
    30601 gagcagccaa atcactggct gcgtgaagag gtacagccag gcatacgtgg tcgtgtttgg
    30661 acgaaacggg agcatgatca gcgtctgtag gacaccgacg gacaggtaga ggaagaagcc
    30721 ggcgtacaga cgggcgagcc cctcccgaag catgcgtaca atcaggatcg cagtcaggaa
    30781 cagactcgta atccaaaggt agtgttcaag ttgggagatc tccggcattg gacacaccta
    30841 acccggcccg ccgggtcgga ggactaccgg tggggtgagt ttggtaccca cacctacgat
    30901 tttggctggt ggcagccaat ccctgctgaa gcgagggccc gcgcggctag cggacgtcgc
    30961 aggggaagca gccgggtctc ggcacgattc tagtgctgac ttccgcgact gccatgaact
    31021 gaactgctag gaacagaatc accagcgctt tctttatcac tttgcacttc tccttatggg
    31081 tgtgtctttt accgggtaca aatgctagcg tatcttgttt tccgccagta gtacgaaagt
    31141 tccaaaaaat gttccaaatt cggaacaaga ggcccctagt gcctcaaagt cgatggagtc
    31201 acgcggtata ctcgtggggt ataatcggga ctacggaccg cgtccatgaa agcccgaaag
    31261 ctgaagggga gtttcgagcg gggacccgct gcggatctct ggcgaaagac tctcgtgcaa
    31321 ataccttccg tattcgggcg gctagtgtac ctggcatccc tccgcgatca gaataccggc
    31381 cgctacgagc atcacggcat gtcccaagtc ttcggagaag acgaggcgga tcagtcgctt
    31441 cgcgagagcc acggccggac tttcgctgat tggctttgtt tcggcctgga acagcagaag
    31501 gccgatctgg atctctatct atctagtttt gaaccagata aacgcacgat ccttgagacg
    31561 tggattcgtc tggcgcccta tcggaacctg gtcccggctt cagtcagaga ggtggagcga
    31621 aggctgtacc tgaccgacct cgaggcgctg ctcgacctgc tcaagaatga gtacgccgtc
    31681 gtttcccctg atccagacgc atagctacgc cggagatctg tccgactacc tcgatttcat
    31741 ctgggtagga gtacacctcg gggttgcaca tcgaagccgg gtgaggctgc actacgagtt
    31801 ggctttcgtt gaggttacac cacccgcaga ggtagccgtc gcgatgctcg aggaagtaga
    31861 tgggccgctc gaactcgttc gtccatccgc tgttcacgat cttccgcttt gtctcgtcaa
    31921 tgagcaccag cgaccccggt tgaatcaacg gatacatggc ccagtcctcg gtcccgatga
    31981 acgcgtaccg gtggctccgg aggtcgattc cgtttaacat catgagcgga agcttccccc
    32041 atcgttggat cagccggctc agataggtcg tacgccggag atcgatgcct ggatcgagcg
    32101 ccagcggaag ctgcatttcg ccttgtccgt tggcctcaaa tccaatcaag tgagtgcgct
    32161 cgacttggac cgaggccgca tccgccggca gaccggcgat ggagacgcca taccatgtca
    32221 aaacctcagc aaaatcaaga cgatagatgg cacaaagaga atacagccga tagagtgtgg
    32281 gcagggtacc cttgttttcg atatccgcga ggcggctgag ggcgattacg aactcgctgt
    32341 tacggtgctt ctccgcaatt cgcaagctcg cttcttcgac gtccctatat cgcaggttca
    32401 accgctcccg gactcgtttc agtttctgtc cggcatcctc catggtatga tccagtctcg
    32461 gctgccctgc tcggacctct gttccgcccg gacgtcgcca actcgcgaac gggagcgcat
    32521 tcggtggaat tatagcgagg gaatgaagcc gagggcaagc attttggcac gagtttgttc
    32581 tgaattcaga acaatagctc ccaaaggaga ccatccttgc gcgtgattgt cgggatttcg
    32641 ggggcgagcg gctcgattta cgggttgcgt ttgctcgaaa agcttcgatc tcggccgtca
    32701 gtcgaaatcc acctgatcat aactcgcgcc ggcgagcgca cgatcttcct ggagaccggc
    32761 aaggtcgccg ccgacgtgaa gtcgatggct cacttctcgt accccgtgga ggacatcggc
    32821 tgccgtctgg cgagcggttc gtatttggca gacggcatgg tcgttgcgcc gtgctcgatc
    32881 cacagcatgg cagcgatcgc gacgggcgtc accgataatt tactggtgcg tgccgctgac
    32941 gtctgcctga aggagcgccg caagctgatt ctgatggttc gcgagtcgcc gttccatctc
    33001 gggcacctgg agacgatggc tgcgctagcc cgtatcggag cgatcatcgc tccgcccgct
    33061 cccgctttct accataagcc ggagacggtg ctcgacatcg tcgatcacag cgtcgaccgc
    33121 gtcctggacc tgctcggcct gcccgacgtg caggcgaaac gctggcaggc aacctagtcc
    33181 gtcataggag attacacgat ggcgaaacgg cccatgaaag ccgcatttca gggagaacga
    33241 ggggcgttca gccagcaggc tgtgcgccag ttgctcggcg acggcgcgga ggtgctccct
    33301 tgccagcgtt ttgaggaggt tttccaaagc ctgcgcgcca ggaaagtcga cgccgccgtc
    33361 gtccccatcg agaataccct cgccggatcg gtgcatgaaa actacgatca cctgttgcat
    33421 ttcgacgatt tcgagatcgt cgccgaaacc agcgtccgca tcgtgcataa tttgattgcg
    33481 tcgcccggag tcactttccg gaagatccgc cgcgtttttt cgcatccggt ggccctgaat
    33541 cagtgcctcg atttcttcac aaatcatcca gatattgaac gcgtgccgtt ctatgacacc
    33601 gccggcagcg tcaagatgat catggaggac gggctcaagg acgccgccgc cattgcttcc
    33661 gccctttcgg ccgaaattta cggagcgaaa atcctgaagc ggtcgatcga ggacgaccgt
    33721 cggaactaca cgcgtttctt tctgctgcgc agacgggacg cagcccggct cgccgccacc
    33781 aaccgttccg ccgcgcgcgg gtggaaaacg acgctggtgt tcacaacccg caacatcccg
    33841 ggaacgctct ttcgggcgct tagcgccttc gcactccgcg acatcaacct gatgaagatc
    33901 gagtcgcgtc cgctgcgggg caagccctgg gagtacctct tctatctgga cttcctgggc
    33961 gacctcgacg agccccgtgt gcagaacgcg ctaagtcatc tctccgagct cgcggatttc
    34021 ctgcgtgttc tgggctgcta cccacgcggc gagtgaagcg ctacagtgtc gatcttgtgc
    34081 tccgtcactg cccgccgtcc gcgcgtttca gatacagaac ggccggtccg gggaaaggtg
    34141 tgcggaaggt gcggaactcg cctccttcta cgtgtccacc actcgagctc ttgtccgagt
    34201 tcacgttgag ccactgcacg gcgaacgcgc ccttcactcc tcgcagatcc actctgaatt
    34261 caccctggcc tcctggctga aagacgacgt attcggaccc cggtttggcc aggcagtaac
    34321 gagtggaagc cagttcgtcg tgcggcgaca tcccggccag gtcgattctc tcggagagta
    34381 accgggtctg gcccatggcg gcacgggcag aatcgtgcca cgttggcgac ggagtcaact
    34441 cctccatgaa gagaacgttc agcccgcgcg tgaagctctt ccagacccag acgttgtcgc
    34501 cacaggtgtg cccgcagagg tggtcggtgt cgttgacgat cactttgccg ctggagttgg
    34561 cgggcgggtc gcccagatag tcgcccggac cgtcgccccg gttgggcgaa atccagtccg
    34621 ccgggctttc aaagagcgtg cgattggtgc ctcctttgta ctggaaagtc atgccaaccg
    34681 gatggcgctt cgacttggaa gcctcatacg catgcacgaa gcggatcatg tggtactgcc
    34741 agtccgtcga gtaggaaccg gcttcgttgc agatctcgta aaggacgttg tccaggtcgt
    34801 tcacagtatc gatgacctta cggacatagg cctcctgcag cgccagcact ttccggcccg
    34861 tgggactgtc ttcgagcgtg ttgtactccg gggcgcggcc atctccgtta gtgtcggcat
    34921 caatcccgtt gatgttgctg tcccggttga acgggtggca gttccaggcg tcgtaaaact
    34981 ggatttcgta cccctcgaac aacatcacgg atacgtacat gccgcgagcg ccggcggcga
    35041 tgatgcgtgc ccgcatgcgg tcgaagtact gcggatcgaa ccgcgtcaga tcgaacttcg
    35101 gcttgccgtc cgccgccaag cccggtccgg tgcggagcca cgggtggaga gcgttgtagg
    35161 cgacgccggc tggctcatcg tctgtccatt tcggcgactc ccaacgccag aggcggaaga
    35221 aattgtgatg gtgggcatcg agaaaatcga ggaaccggct gtaatcgaag acaggcggcg
    35281 gctctccgat gcgcgatcgg tgccccgtgt ccacgaagtt gttccagttg tgggttcccg
    35341 taaggtagac ggcgcgcccc gaaccatccg tgaagtagcg aggattggac tgcgacggtc
    35401 gcaacagacc ggctggcggc ctgggctgag ccagtacgca catcgcaaat gcgcagacaa
    35461 agaatgcgac taaagctgat cgcggcattg ttggggagcc attttaacgt aagagtcagg
    35521 ccgccggcac cgtcagttca gtacaacgcc ggtgcgccct ttggccgcgt cttccagcgc
    35581 cggtggatcc agagccactg gtccgggtgc tcccggatca cggcctcgat tgacgattga
    35641 aggcgttgcg tgtctgcggc agggtccccc gtgatctcta ccggcggata gaagcggagt
    35701 acataccgcc gctcgacgtc cgaccagagc gcgaaccccg gaatcacggc cgcgccgctg
    35761 tgcgcggcaa gacgcgcgat ccccgggttg gcacacgctt ggattccgaa gaaatcgacg
    35821 aagacgcctt cttcgaggcc ggtgttctgg tcgatcagaa tccccaccgc ttcgttcgcc
    35881 gagagcgcct tcaaaattcc ccgcgcgaag tccttcttct ggatcacgtg gttccccgat
    35941 gctgtccgat agctgcttac gagtgcgtcg attttgggat tgtccagcgg ccggaccacc
    36001 acgtgcatcg gcgccgccat cagcgcgtgc gcaaacgcgc tcagctccca gttgccgaga
    36061 tgcgccgtcg cgaaaagcac gcccttgccg cgtcgaaggg cctcctcgaa atgttcgaag
    36121 ccctcgtacc ggatccactc cgagacgttc tcgcgtccca tcttcggaaa ttgcgcgaag
    36181 gctgcgagca gcctggcaat ggagcggaag actccatcga tcacttgttc ccgctgccca
    36241 tcggacagtc ctggcagcgc catcgacagg ttccggcgcg ccacgcggcg caatcgcggg
    36301 agaacgagat ccagaagccg cgcataacct cgcgccagcc gttctgccgg ccaacgtggc
    36361 agacatgcca gcgacttcag cacaagggaa gccagtgcgt attcgacgct gtcgcggaaa
    36421 acggagcggc cgggcaagcg tctattctat ccagccgccg cccaacacga ggtcgccgtc
    36481 atagaagacc gccgcttgtc ccggtgtgac tgcgcgctgc ggctcgtcga accgcacctc
    36541 gatccgggcc ggctcgcccg ttggggacac cattgccggg gcggcagcgt gcttgttccg
    36601 gatcttcacc ctcgcacgga caggctccga aagcgaagcc caggggaacc agttcacctc
    36661 ccgtgcggac agagtgtcgc ggaacaattc ttcggaccgg ccgataatca cgcgctgcgt
    36721 cgcgggcacg gtggcgatca catagagcgg ctcgcccgcc gccactccca gcccgcggcg
    36781 ctggccgacc gtgaaatggt gcaccccggc gtgctccccg aggacacgcc cgtcggtgcc
    36841 cacgacctcg ccacgcgtct ggcgtgatgg aattccctgc tcccgaaaga atgcctcgat
    36901 gaacgcgccg taatcgccgt tggggacgaa gcagatctcc tggctgtcgc ccttgcgcgc
    36961 caccggcagg ctcatctcct cggccattcc acgcacctgg tctttggtgt acccgcccaa
    37021 cggaaagagc gtgcgtgcaa gctgtgcctg gctcagcccg aacaggaaat acgtctggtc
    37081 cttggcgtcg tccgcgccgc accacagcaa ataccgcccg ctcttctcgt cctgggagac
    37141 tctggcgtaa tgcccggtgg cgatcttctc cgcccccacc gaatccgcca gctcgaggaa
    37201 ctggtcgaac ttgatgtggt tgttgcagag cgtgcacggg atcggcgtgc gcccggcgag
    37261 atagtcatcg atgaacggtc gcaccacctg ttcctcgaat cgctcctcga agttcaccac
    37321 gtagtacgga atgccgagcg tctctgcgac gtgccgcgca tcgtagacgt cgtcgagcga
    37381 acagcagcgg cccgtggcgt tttccccggc cagttccggc agccggcgct ggttccagag
    37441 ctgcatcgtc agccccacga cgttctcgcc gcgccggcgc agcaggccgg caaccacgga
    37501 gctgtcgact ccgccgctca tcgcaaccgc ggtgatcttc tcaggcatag gccggcgaaa
    37561 gcttgcgtag acgggcggcg gacgcggcga ccgccccgat aagcgcgtcc acctgctctt
    37621 ccgtgttcga gcggccgagc gagaaccgca agctcgactt cgcgtccatg gcagaaaggc
    37681 cgatcgctgt cagcacgtgc gacggctcaa ccgcgccgct cgagcaggcg gcgccgctcg
    37741 aaacggcgaa gcccatgagg tcaagcgcga tcaccatcgc ctctccctca atcccatcga
    37801 accggatgtt ggtcgtattc ggcgtccggg gcgagcctgc gccgttgacg tgcgcgtgcg
    37861 ggacgcgcgc cagaataccc tgctccagac ggtcgcgcaa cgcaccgatc cgtacggcct
    37921 catccgaaag gtgtgccagg atccactccg cggccgcgcc gaacgccgcc gctccgggaa
    37981 cgttctccgt ccccggtcgt cgatcgcgtt cgtgatgccc gccgaacagg accggctcaa
    38041 ttgccgtccc cttgcggata tagagcgagc ccgcaccctt cggagcgtaa atcttatgcc
    38101 cgctcatcga atacagatcc acgccgagcg ccgcgacgtc caccggagtc ttcccggcgg
    38161 cctgcacgcc gtcggaatgc atcaacgcgc cggcgtctcg ggcgatccgg gcaatctccc
    38221 cgactggctg gatagtccca agctcgttgt ttgcgtgcat caccgatacg agcaccgtgt
    38281 ccggccgcag cgcgcggcgg acttcgtccg gatcgaccac gccgctcgaa ccggcgcgaa
    38341 catacgtcac ctcaacgcct tcgcgctcga gctggccgca tgtattgagc accgccggat
    38401 gctcgatcgc cgtggtcacc acatgtttcc gcggtttcgg cgaccgccgc accgcgccga
    38461 gaatcgcgag attgtcggcc tccgtgccgc cactcacaaa gacgacttcg cgcgggcgcg
    38521 cgtggatcag cgccgccgtc tgccggcgcg ccgtctccag ccgctgcttc gccacctgcc
    38581 cgaaatggtg gatgctcgac gcgttgccat acacctcccc cagcagcgga accagcgcct
    38641 ccagcacctc cgcggacaca ggggtagtcg cgttatggtc gaaatagaaa cgatccactg
    38701 gaattctgct ttaattttag cgtatgcccc gcggcgtcat taccctaacc accgatttcg
    38761 gcctctccga ccatttcgtc ggaacgatga agggcgtcat cctgagtatc cacccacagg
    38821 ctacgatcat cgacatcagc cacgggatca ctccatatga gatctccgag gcggcgttcg
    38881 tcgtcgccca agcctatcgt tactttccac ccggcacggt tcacgtagtt gtcgtcgacc
    38941 cgggcgtggg caccgctcgc cggccgatcc tgatcgaagc agcgaagcag tttttcatcg
    39001 cgccggacaa cggagtgctg tcgatgatct gttcgcgcga gaaacacagg gcgcgagcca
    39061 tcacggcgaa gaagtacttt cgaaagcccg tcagccagac cttccacgga cgcgacatct
    39121 tcgccccagc ggccgcgcac ctcgcgaagg gagtggcgct cgcaaggttc ggcaagccca
    39181 tttccgacta ccttcgctcc acgttcgaaa agccggttcg gaccggcaag cgcatctgga
    39241 cgggaaccat ccttaagatc gaccgcttcg gcaacctgat cacaaacttt cacattgacg
    39301 aatttccggc cgtccgcgag cggatgttcg agatgacggt cggaccgcgg cgcttgtcgt
    39361 atctcgcaaa caactacacc gagtgcggct tcggtgagct gttcctgatc gtcggcagct
    39421 ccggctacct cgaggtctcc gccaaccaag gctcggcggc aaagatgatc ggctgcgcca
    39481 ccggcaccca ggtggacctt accctttact gacagcggtg gcacgcggca gtggagcagg
    39541 ccatcgccgt ttgtggcctg ctgcgtcgcc actttccgaa gccgcgaccg cagaaagcgg
    39601 tcataaagaa ccgcgagcgt cagggagcgg actctttcag tagccccttc gctcctttcg
    39661 tacaatgagc actcatgccc gaactccctg aagtggaagc agtctgccgg aaactgcggc
    39721 gggatgccct cggcgcctgt atcgccgccg cccgcgtcct ccgctccggg acgacccgcc
    39781 cgcagaaaac gcgccgggtc gagtcgctgg ccgccggccg catcatcgaa actatcgagc
    39841 ggaggggaaa gaacatcctg atcggattgt ctggcggcat gtgggttcgc gtccacttgc
    39901 ggatgaccgg taacctccat gtgatccccg atgtccactt ccggccggct tcgacccggg
    39961 cgtacttcga actggaaggc ggccgcgggc tgattttcga cgacccccgc gcgctcggcc
    40021 ggctgcacat ccatacgact gcggaagccg cccgcctgat ggaggacctc ggccccgaac
    40081 cgctctcgcc ggtgttcacg ctggacgctc tggcagcggc cgccgggcgc tcgcggaaac
    40141 ccgccaagct cttcctcatg gaccagcagc atgtcgcggg tctcggcaac atctacgcag
    40201 ccgaggcgct gttccgggcg cgcatccacc ctgcccggcc gatgaatcgc atcggaccgt
    40261 cgaggctcgc cgtgctgcac tctgcgatca tcgaagtctt acaggaagcg gtgcactggg
    40321 ccgtgaggtc ttacggccgt cctggcgagt tctccgaagc cgaggacttc cccctcgccg
    40381 tctacggacg cgaaggcgag ctctgcgtca cgtgtgaccg gcgggtccgc cgcatcccgc
    40441 agggaggccg gtctacctac tactgccccg gctgccagcg ctgagccgac cgccacgaga
    40501 accagtgacc cggccagaac tctcgatgtc acaatacaag agtttaaggt ttttaagact
    40561 ggttctagaa ctctcaattt tctaattcta gaaccttggg tcttgtcaca cgagactggt
    40621 acgtctataa ctgagggtgg gtggaaggag gacgacagaa aatgccagat cgaagatatt
    40681 ttctgaaagc tctcgcattg ggtggagtcg gcttgttcat gcgcggcaaa ggcggcccgg
    40741 cccttgcgca gatccacggc ggcactctgg atccgtccaa tgttccaaag taccagacgc
    40801 cgatgctcat tccgcccgcg atgcctaagg ccggaacgat cgtgcaacgg ggaggcaagc
    40861 ccatcgacta ctacgagata tcgatgaagc agttcgctca gcagatcctg ccggccgggc
    40921 tcccgccgac aactgtttgg ggctacggcg cagttgctgc ggacagcagg cggggacttc
    40981 tgctccacaa cgcgccgtca ctgaccattg aggcccagtg gaaacggccg gtgcgagtga
    41041 agtggatcaa cgacctcgtg gatggtaacg gcaactaccg gccccacctg ttgccggtcg
    41101 atccgacgct gcactgggcc aaccccccgg gcggcacggc gggtcgcgac atgcgcccgg
    41161 agttcaggtc cacgcccgga ccgtacaccg gccccgtccc gatcgtcacg cacgtccacg
    41221 gcgcggtcca cgtcggtgac gagagcgacg gctacgcgga ggctt
//
LOCUS       DATIFQ010000405        32800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1475395, whole genome shotgun sequence.
ACCESSION   DATIFQ010000405 DATIFQ010000000
VERSION     DATIFQ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 32800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32800
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475395"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VLE22_20045"
     CDS             complement(<1..185)
                     /locus_tag="VLE22_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16754.1"
                     /translation="MTKAVLTAFAVIAAMNLSAQTIDRTKPPETGPLPAFKLPPVFET
                     KLDNGLHVVLVEDRRFPL"
     gene            complement(192..1571)
                     /locus_tag="VLE22_20050"
     CDS             complement(192..1571)
                     /locus_tag="VLE22_20050"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSB16755.1"
                     /translation="MRSIFAMLVLCAGAAVVSGSDFRIPFEKYQLKNGMRVILAPDKA
                     VPVVSVYVIYGVGARSEEKGRTGFAHLFEHMMFQGSANAGKGQHFKQVESNGGTLNGS
                     THPDFTDYFEVLPANKLAVGLWLESDRMRSLAITDDNLTNQKEAVKQERRLSFDNQPY
                     ATAIVDRWPLIAFRNWSNSHSLIGSFEDLNAATVDDVARFFKVYYAPNNAVLVLSGDI
                     DIPAAKKLVETYFGDIPAQPQPKHPDLSEPSEVQAQSEVYRDPLAQVPAVVVGYPGPE
                     RRSAEFYALAMLDVVLTGGESSRFQQNLVKGKRSVVQFEANLGWPFAASSDYKHPGHY
                     AMLLLHNPKFSGKEIAGQVQEEIAAIQKDGVPAKELARVRTFLRSSRISGLQSSLRRA
                     MLLGQYELLDGDPDYINSEMDAFLSVTPEQIQAAARKYCVPEKRFTLEIVPAPPAEKA
                     EPSAQKTGK"
     gene            complement(1634..3361)
                     /gene="polX"
                     /locus_tag="VLE22_20055"
     CDS             complement(1634..3361)
                     /gene="polX"
                     /locus_tag="VLE22_20055"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HSB16756.1"
                     /translation="MENREIARLLAETADLMEIAAEDSFRIRSYRNGASAIENHPERI
                     VDILKDPDRSVTEIPGIGKGLAAVLAEIAERGSFERRDELLAKYPPTALEFLKIQGLG
                     PKSIALIWEHYRVSTIDDLERLCLEQKLRVLPRMGAKLEEKVLRSIAQYRKRVGRFLL
                     SFADELARELSEYLSSEPGVEQVTPAGSLRRGKETVGDLDLLVTGPGATATIERFIKH
                     PKVSEVLGKGGNKASAKFGLEGMQVDVRALPRESYGAALQYFTGSKEHNVALRTRGVR
                     MGFKLSEYGLFRTDDESRVAGETEEDVYRALGLAWIPPELRENLGEIDAAEEGRLPNL
                     VELGDIRGDLHMHTIESDGRATLEEMAQAARELGYKYIAITDHSKALAMANGLDEKRA
                     VSFARQVRELNKDGLGIRVFSGLECDIRRDGSMDLAGDALAELDLVIGSVHSYMSLES
                     AEMTDRLLRALESPHLRVLGHPTGRVLLHRDAFPFDFERVAAEAARRGVCFEINASPE
                     RLDLDAPLLRSARAKGVRFTISTDAHHPKHLTNMKYGVLTARRGWLAADDIMNTRSLE
                     QFTRFLRKK"
     gene            complement(3402..4328)
                     /locus_tag="VLE22_20060"
     CDS             complement(3402..4328)
                     /locus_tag="VLE22_20060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSB16757.1"
                     /translation="MGDLQRTGRRVAIASMAVSGVLAASKIVVGLLVNSTSVFADGVE
                     SAGDMGTSAIVLFGLLVAAKPPDANHPYGHGRFETLTGLLVGLVLVATGTGIAIHSLE
                     RVNFVHTAPAAYAIWPLLASIAAKTVMSRLKFYYGRKIHSASLTADGWNDMVDILSGS
                     TALAAVGLTLYNPGRFLAADHYGGFAVGLIVIFTGVRVVRETTMQLMDTMPEPDLMRQ
                     IRSTALSVPGVLGVEKCYARKTGLQHHVDLHLEVDPQMTVRSSHDIATQVRIRITKVL
                     PWVADVMVHVEPYPPLLNEAPVAEGQEAHRPS"
     gene            4418..4720
                     /locus_tag="VLE22_20065"
     CDS             4418..4720
                     /locus_tag="VLE22_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16758.1"
                     /translation="MTLTDVRKLAIRRQARIRFSVSAGTECVIDEHGVVRVAGLDGPP
                     SFSLEEGFSGAGQFVLEPVPAAGVKNAPLRSLCRDELEVLLSDVTPAAQKTSDDEG"
     gene            4807..7173
                     /locus_tag="VLE22_20070"
     CDS             4807..7173
                     /locus_tag="VLE22_20070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16759.1"
                     /translation="MRLSFRIVFVLCFFTFLLPAQIEVSTSTLSFEAIAGGSPPPEQR
                     VYVIASPGGTLTEVALAADGGAPGLLAPKWLVVAPKSLTMPGAVRVAVDPSTLVAGTQ
                     ASARILFTKIDGTALGPQVQVTLNVRESAPKLVVTPETLHVYGKATTSAFVESDIVLR
                     NTGGGSLAPVTAEVKSAAPWMSVKVLEPCAVECTVRVTTRYGMETGTRFAVVGLNTAA
                     GAARLPVALHVSGDGSILSLEPPTIALEARSGHGLIRSRYSLVYSNGDTPTDWIVEKI
                     AGGAWLTLDSTIGVSSRSIPGTVSVSANSAALVPGTYYSLLRLSSSGALNSPLYLPVV
                     LRIAETAEPPIPLLPLGGYVFTAKIGENPPPGLKFNLSASSATPVQFRASVDHVTGAG
                     WLTVSPTSGSVSSTAAASINVSTTVLPKGVYTGKIHFSVGNLGIRSLNVTTISSPASG
                     CVPGSVHITHLGIPDDFKAAVGAHLPLVVSVLNDCGEAVTNAAVTVEFSNGERSLPLR
                     HIANGVYAEAWSPSKEAAALNITAVATSSAYPFVSRAMVAGSVTSGSGPVLFANAVLN
                     NLNPLVGAPLAPGTVAQVYGKNLATATVQPGFVNGKLPSTFNETSVLVGDIPAPLYYL
                     SDSQLNVQLPVELASGREYQALAALNGAWSVPVTVFTEPATPGVVVYSDSGVIAQHAD
                     YSLVNAASPARPAEYIRVYLVGMGSTNPVVATGLPAPPQLHRVVKQPDVTLDGAPVTV
                     SYAGLAPGWVGLYQIDFQVPASAQTGNLKLTITQNGVLANDSYLPVRR"
     gene            7268..7579
                     /gene="hfq"
                     /locus_tag="VLE22_20075"
     CDS             7268..7579
                     /gene="hfq"
                     /locus_tag="VLE22_20075"
                     /inference="COORDINATES: protein motif:HMM:NF028518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HSB16760.1"
                     /translation="MEKLAQNIQEAFLNNARKEKTFLTIYLMSGVKLSGRIKSFDKYS
                     VVLETNNQEQLIFKHAISTLVVSKPTHTYAAVTHAQAGPPVVGGPHPAPGVPSHESTE
                     E"
     gene            7592..8917
                     /gene="hflX"
                     /locus_tag="VLE22_20080"
     CDS             7592..8917
                     /gene="hflX"
                     /locus_tag="VLE22_20080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSB16761.1"
                     /translation="MKESDERAILVGLELRGRTGSLAAGKAESFRYSSEESLEELAAL
                     AESAGAAVVEHVIQSRPAPEAATLIGSGKVRELAQLTGASQADVVIFDRDLSPTQQRN
                     LERALSVKVVDRTQLILDIFARRARTREGQLQVELAQLSYLLPRLVGRGVEMSRLGGG
                     IGTRGPGETQLETDRRRIARRLKKIRVELESVRGHRSVQRKQRQAIPLATAALVGYTN
                     AGKSTLFNRLTGAGVLADDRLFATLDPTIRAAILPSRRRILLSDTVGFIRNLPTTLID
                     AFRATLEEVIEASLLIHVVDASSAHAVHQTSHVRKVLTEIGIANTPQVLVLNKADSLP
                     AGGIDRETVHRRLAEDLGAIVQARAVSISALTGAGIERLLEAIDETLPLDPVERVHFR
                     LPAGEGAALHLLHEFGQVRAADYADGYCNVEADVPESIRRRLTRYLVES"
     gene            9112..9783
                     /gene="pgsA"
                     /locus_tag="VLE22_20085"
     CDS             9112..9783
                     /gene="pgsA"
                     /locus_tag="VLE22_20085"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HSB16762.1"
                     /translation="MNLPNLLTILRIFFVPFLVAVLVQGKLGIDVGGFHLSNDILALG
                     IFLAAAATDLLDGYLARKWGQVTTVGMLLDPIADKLLISAALIALVQIRLIPAWMVIL
                     IVGREFAISGLRSIAAAEGYTIRASDLGKTKMMSQVIAISLVVLSIRWRQLAPYANLW
                     LWGVVVFSLASAVQYFRKFWRRVDDQIKLRGRRELLLLERLAKREQRKRERAAAAERA
                     AGQGL"
     gene            9811..10857
                     /locus_tag="VLE22_20090"
     CDS             9811..10857
                     /locus_tag="VLE22_20090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB16763.1"
                     /translation="MNHEADAEIAVIVVNWNRRELLRRCLESLARQEGVDFEIIVVDN
                     GSVDGSPDMVKRQFGAGPVPLRLIENAENRGFCGANNQGICATRSRFVALLNNDAEAA
                     AGWLAALRRCFDGRPDVGMVASKILVFEDPRRIDKVGHLIYPDGQNRGRGTGEFDTGQ
                     YDRIEETLWPDGCAAMYRREMLDEIGGFDEDFFAYADDAELGLRARIAGWNCLYVPDA
                     VVRHHRGATLGVASSRRLELIERNRILLAAKLFPWSLLWLNGLYYTARIGAGMVAAAR
                     GKGEIGRFPGIREKLRAAMALLRGDLQALRMLPRMLRKRREIAKFRRLSSSQIRRLIL
                     HHRIPLRKLTEEST"
     gene            10998..11828
                     /locus_tag="VLE22_20095"
     CDS             10998..11828
                     /locus_tag="VLE22_20095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB16764.1"
                     /translation="MIRLHPRPTPEEIAQYYPADYWFAPDDAAADWLEEIYRRFVLRD
                     HVRFVRRAVESSRVQGPVLDVGCGGGLFLRMLAERGLPVVGLDFSLDAAVLSWRVNGV
                     PAVCATLSQAPFPSESCAAVTMFHVLEHLYDPVSYIEAAHRLLKPGGRLIVQVPNAAC
                     WQFLLLGENWTGVDVPRHLYDFRASDLEVLLSDCGFDVVRQKYFSLRDNPAGMATSLA
                     PSLDPMVRRVRRTQETSRIRLFKDLIYFGLVVCCLPFTLIEGACRAGSTVMVEARKKA
                     "
     gene            11825..12514
                     /locus_tag="VLE22_20100"
     CDS             11825..12514
                     /locus_tag="VLE22_20100"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB16765.1"
                     /translation="MNRAAILRCLPGPLRRHILFFEAEIERAVAVFARSLDPGSRVLD
                     AGAGEGRYAGFFPNQRYCGVDLGIGDAAWNYRGLDVVADLAALPFPNGCFDACLNIVT
                     LEHLPDPGAALVEIARTLKPGGRVLLAVPHEWEVHQAPHDYFRFTRHGISHLLERAGF
                     SSVDVEPAGGFFRLLSRRLLNGLQFFPPLLVPVAALFLAPPALLLPGFDFLDRERNFT
                     LGYLCTAVRRS"
     gene            12490..13356
                     /locus_tag="VLE22_20105"
     CDS             12490..13356
                     /locus_tag="VLE22_20105"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSB16766.1"
                     /translation="MHRRQALLTLLRALAPPAGFNGDSALRYTVKAVSFGPRPPGSDA
                     IHRLQAYILSQLKTTGCEIIEDDFTARTRFGPVAMKNIIARFRGTSGRALAITGHYDT
                     KLMRGVPFVGANDGGSSTGFLLEMARVLAGRSNEDDVYLVWFDGEEALVAWTEADGLY
                     GSRHLARRWSDDRTATRLKALINVDMIGDRDLGILQEMGSSPGLRKLVWQVAEEIGYG
                     RYFLDVSGNIEDDHAPFAAAGVNALDLIDFDYGPGNSYWHTAQDTVDKLGAHSFQVVG
                     DVLLEVIRRLER"
     gene            complement(13423..14328)
                     /locus_tag="VLE22_20110"
     CDS             complement(13423..14328)
                     /locus_tag="VLE22_20110"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSB16767.1"
                     /translation="MRRKLRFWGAGVLFALAVTAFFSGPAFSRGGNDFHFAILGDRTG
                     SAMPQIYGRVWREVAMLSPDFAINIGDTIQGGDDTRAETEWQELRAILRRYASFPFYF
                     TPGNHDIWSDKSRELFERETGRRSFYSFNVQDAHFTVLDNSRTLDLDEEQLKFLEKDL
                     RRNRHRRPKFIFFHKPYWVVFLKAGSGEFPLHRLARQYGVDQIISGHGHQFMRMERDG
                     IGYVALGSSGAHFRRDLTPDQGFEQGAFYHFAWVRVKGTKVQVTIKEIGGPAGQGRMF
                     RAEDWDADGPKFDTADPALKDEPET"
     gene            14631..16088
                     /locus_tag="VLE22_20115"
     CDS             14631..16088
                     /locus_tag="VLE22_20115"
                     /inference="COORDINATES: protein motif:HMM:NF011305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HSB16768.1"
                     /translation="MHTLDFAAAGLLIPLAILMLVSGLDDLLLVLAMAWRWVRLRRLP
                     QGPSDADLQLVPEQRIAIFVPLWREDRVIRRMLEHNLAAIRYRRYDFFVGAYPNDEPT
                     LDAVRNAEQRYPNVHLAVCPHDGPTSKADCLNWIYQQMLVFEDLRGVYFDVIVTHDAE
                     DLIHPDSLRWINYHSSSCDMVQVPVLPLATPLRSVTHGVYCDEFAEYQTKDVPLRTVF
                     GGFLPSNGVGTGYTRRAVWKLAEMSSNRIFEPGCLTEDYDTGLRLHRLGFRQMFVPLR
                     FAGGRPLATREYFPQSFRQAVRQRTRWVIGIALQSWERHGWKGGPSVAYWFWRDRKGL
                     LGHPVTIAGNALFAYGLASWLWSRVTGLPWNLSHHVAQPLFTRLLVATTSLQALHMLV
                     RAACSARIYGWRFALGVPVRTVWANWINSVATTLAVCRYVWARLERRPLVWLKTDHLY
                     PSREALRQHRPPEPAVWTSDHVDVGSIPPAGAQVR"
     gene            complement(16076..17254)
                     /gene="argJ"
                     /locus_tag="VLE22_20120"
     CDS             complement(16076..17254)
                     /gene="argJ"
                     /locus_tag="VLE22_20120"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HSB16769.1"
                     /translation="MKLPLGYRYSSTYAGIRKDKRDDLAIIASDAPASAAAVFTTNRV
                     QAAPVRLARNHLKATGGEARAILANAGNANCATRTGMRVAAACCRAAAKELKSPMTQV
                     LPASTGVIGVEMDASLVTGSLPALFKGLSETRFEDVSRAIMTTDRIPKVAFGEVPFNH
                     GMARIAGMTKGAGMIHPRMATTLGFVMTDAEIEPGPLARMLRHSTERSFNRLSVDGDM
                     STNDTLILLANGASGLRPTSKERMVFEEVLCWVMQDLAEMIARDGEGANKKVTVHVVG
                     AKDDKAATQIARAIANSPLVKTAIAGSDPNWGRILSAAGASGVEFDPDKVDILLQGVP
                     VCRRGLAAEFSELELKKKLDTSDCVIRFAIRGQGRGETCFWTCDLTEGYIRINASYRT
                     "
     gene            complement(17355..18284)
                     /gene="fmt"
                     /locus_tag="VLE22_20125"
     CDS             complement(17355..18284)
                     /gene="fmt"
                     /locus_tag="VLE22_20125"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSB16770.1"
                     /translation="MRVVFLGTPGFAVPTLERLIEAGHEVSAVYTQPDRPKGRGGEIA
                     ASAVKIAALRHGLPVYQPERIRRPEIVDALAALKPDAMVVVGYGQIIPQNIIDIPPQG
                     IINVHASLLPKYRGAAPVQWAISHGEPVTGVTTMRIDAGLDTGDILLQRETAIGPEET
                     AEELGRRLSSMGAELLEETLRDLEAGSVVPRKQDPSLATLAPILRKEDGRIDWNLPAA
                     DIYNRVRGFVPWPGAWSVFRGQLLHVWRATTGDAAVSEAPGTLKPARRRLLVACGQGT
                     SLEVIELQLEGRKRVTSEAFVNGQRLAENERLL"
     gene            complement(18473..18988)
                     /gene="def"
                     /locus_tag="VLE22_20130"
     CDS             complement(18473..18988)
                     /gene="def"
                     /locus_tag="VLE22_20130"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSB16771.1"
                     /translation="MIYPIVKLGDPVLEKPAETVHEFDTPDLHKLIEDMFESMYAAKG
                     VGLAAPQIGISRRIAVIDTSVGEDGSQKLVLINPEIVRKEGKQTSEEGCLSVPGFREE
                     VTRPLRVTVRAQNAKGETFEMTGEEVLARAFLHETDHLNGKLYLSHVSVLKRDLIRRK
                     VRKMIKAGEWS"
     gene            complement(18988..20160)
                     /gene="aroC"
                     /locus_tag="VLE22_20135"
     CDS             complement(18988..20160)
                     /gene="aroC"
                     /locus_tag="VLE22_20135"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HSB16772.1"
                     /translation="MFRFETAGESHGECLVATLTGLPAGVPVKLDTVDRELWRRQQGY
                     GRGGRMKIETDHVEIVAGVRHSYTIGSPVAMLIKNKDWENWRDALAVEDTDGGEEKRK
                     VVTRPRPGHADLAGVLKYDFHDARYVLERASARETTARVALGAIAKSYLREFGIEVLS
                     HVVAVGGVCLGRPATWQELVALSRSKEVLLGCVDAEAEANMKEVVDQAYRTGDTVGGI
                     FEVVAHNVPPGLGSHISWDSRLDGRLAQAIVSIQAVKGVEIGPAEENAASFGSKVQDT
                     IHYDLESRRFARGANRAGGLEGGITNGQDVVVRGFLKPISTLRRPLESVDLITREAVQ
                     AAYERSDVCVVPAAGVIGEAMTALVLAQAMLEKFGGDSMREAKRNFDGYMEQVRRF"
     gene            20235..21233
                     /locus_tag="VLE22_20140"
     CDS             20235..21233
                     /locus_tag="VLE22_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16773.1"
                     /translation="MLKLCMHANTRVLSLLLFAAVTPLFSEVALLVGEPFGKFGALNP
                     TGHAAIYLSGVCAESPIKLRLCRPGDTGVVISRYNRIAGYDWIAVPLVPYLYAVERPE
                     DVPASADDLAVARLREGYRRSHLWTLVPSGPENDPASGDWTQLAGAAYDRTIYGFALE
                     TTPEDDARLVRYLNSKPNRKRFNLLYRNCADFARGILNFYYPRALRRSRIADAGITTP
                     KHSAKTLVNYCRRRPGLRLSQFVIPQVPGGRPSKKLRGVSESLVWSKKYVAPLLLFQP
                     WVAATAAAAYLSKGRFDPARHCETVCEPGTLESCISGSGTGTIAASADRPARLSRD"
     gene            21299..22759
                     /locus_tag="VLE22_20145"
     CDS             21299..22759
                     /locus_tag="VLE22_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16774.1"
                     /translation="MSDRGPLEDLVRRARRRIVRRLVAAELAFAAAVAMAGLIALLVL
                     GTQILEWYWLVVLFGGSLAWGLYRTLRRVPSAYYVARNVDRGLGLEDSLSTAYHFLNP
                     PGSLKVSPDVRDAQRRQAEELAGRADVKQALPLGVPRGLRAAGLLGLVAFGLFALRYG
                     LTHSLDLRPPIARASFDVFSAKTVETAGLPKPRPPRPGNEPIPAIGMTADERAAKHDS
                     RQSLATEPGEAEASSVPGDALRKAEGLPTRDAADEQAEASSGEPAPNADSSPAAEGDN
                     AAPSSPRKQNAKSGENSDLLNKLRDAMADLLSRLKIKPPSSGEMQTAEGKGDTESRQR
                     QGKGQKGPPGASREDMRADANAEQEAGEESEGAESRDKAAGKPGRRSADTAANRKERS
                     GVGKEEGDKEIREAQQLEAMGKISEIFGKRAENLKGEVTVEVSSGDQQLKTPYSQSAA
                     AHSDTGGEIHRDEVPLIYQQYVQQYFEQVRKLPPPK"
     gene            complement(22856..23893)
                     /locus_tag="VLE22_20150"
     CDS             complement(22856..23893)
                     /locus_tag="VLE22_20150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB16775.1"
                     /translation="MSSRCLPLLAVLAAGALAIPGCRKSPPANVAATVNNRVITHEDL
                     ERQYKLQFPQRDERASEDLLAIQKLEVLRSMVDNEIMLQRAEKLGLMAVDSDVDAKFN
                     ELKAPYTQEEFQKQLEARNMTVAELKTQLRRDLSIQKLFNKEITSHINISDKDVTDFY
                     NSNKAAFNQAEPRVHLLQLLVTPGANPDARNLKRDEANSEEQARKKIRMLEARLKQGE
                     DFAMLAQNYSEDPNTAANGGDLGFIPESALEKANVELRKMVMQLQPGQISPIIRTPEG
                     YRILKVMSKEPAGQRELQDPRVQQTIRETIMNRKDQLLKAAYYEVVRGEAQVVNYYAA
                     SIAERMRTTGK"
     gene            23960..26074
                     /locus_tag="VLE22_20155"
     CDS             23960..26074
                     /locus_tag="VLE22_20155"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSB16776.1"
                     /translation="MHRTHGFITFTAACFSLLAAPRQTPGVPSGRSSFEWQTATAAEL
                     GLDPAKLDAMKDALAARGTSGLLIVRRGKIAYEWYAPASGPAKPHYTASMAKALVGGM
                     SLLLALNDGRLGADDVAAKYIPEWRNDPRKARITIRQLASHTSGIEDAEEEGKRHDEL
                     TGWKGAFWKRDPDPFTIALTRAPVVFDPGTANAYSNPGMAALAYAVTAALRTAPQTDI
                     RTLLKQRVMDPLGIPEKEWSIGYDRAYEVDGLSLYANWGGGGYTARAAARVGQLMLHR
                     GEWNHRRLIGREWVRQALAPSGLPDQPSTAADPAPASGLAWWINVKSGWPRVPRDAFA
                     GAGAGHQLLIVIPSLDLVVVRHGEALEPPKAGGSFWAPVVRYLLDPLMDAVEVKTPYP
                     PSSVIRKIDFAPESTIVRKALGSDNWPVTWGDDGEQYTSYGDGWGFEPLLDRKLSMGF
                     ATIVGGPKDFAGVNIRSASGERTGDGKEGAKASGMLMVGGVLYMWVRNTGNAQLVWSS
                     DRGRTWKWGFRFDTAFGSPAFLNFGRNYAGARDNYVYTYSQNGPSAYESDDALLLARV
                     RRNKIRERSAWEFFERLDTAGHPVWTGDIARRGEVFRYPGRCQRVDIVYNPGLKRYLL
                     ALGFDHSGGWGIFDAPEPWGPWTTAFHTDDWGLGGTHGYRLPAKWIGAAGTEMYLIFS
                     GANRPGITTDAFCLRRLTLTMK"
     gene            complement(26093..26713)
                     /locus_tag="VLE22_20160"
     CDS             complement(26093..26713)
                     /locus_tag="VLE22_20160"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB16777.1"
                     /translation="MLRVYSRRLGLRIWAYCLMPNHVHLVCVPEQPLSLARTLGQAHR
                     DFARHFNIVRQTCGHVWQARFFSCPLDGIHLWRAVAYVERNPVRAGLVREAGQYRWSS
                     AAAHLTGMPDPKGLLDLGPWAQEYTPERWRMVLASSVAEEALAERLREATLRGRPLGS
                     EEFIDELERKTGRQLRPLPAGRPKKPRTESAAAGADGRQLPLEIRK"
     gene            complement(26896..28197)
                     /locus_tag="VLE22_20165"
     CDS             complement(26896..28197)
                     /locus_tag="VLE22_20165"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HSB16778.1"
                     /translation="MRRRSFLASAAAAPLAACGVSRAPKEESRQAAMNIAEARKKYRH
                     DLFEEYLPFWEKFGVDHEHGGFFHSLDYDGTRVKTDKFHWFQGRGVWVYSFLYQHFGK
                     EARYLEIARKAKDFMLKHGPQPDGWWAETLTPEGKLVRPFAGDVYGMYFGAEGLQEFA
                     AATGDAQAHELSLGLMKKLWSYINSPSVQLPGTGRPGVRPQGMWMVNLRIATQMLRRW
                     KDPEIAEIAERCVDAIINRHYNPDIGLNNELLNFDFSRPPEEATKSLLGHSAETLWMV
                     MDEADRRGDSKLWQTAAERIRRHLDVGWDYVYGGLAQWINVDQGCYQWPADRPVGTDL
                     EFRGTGEYFYMKPLWALNEVLIAALNIYERTRAEWAARYFTMAQDLIDRKYSARPHGK
                     SGYMLFADRKLTFQPHVTRQDNYHPPRQLMLNLLTLERMAT"
     gene            complement(28349..31642)
                     /locus_tag="VLE22_20170"
     CDS             complement(28349..31642)
                     /locus_tag="VLE22_20170"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB16779.1"
                     /translation="MFARREGIVRLAIFASLFVFWAIQVMAQSTLGTIRGSVTDSTGA
                     LVPDVNVVVRNVETNIERQTTTASTGNYEVTQLIPGLYEVTAEKAGFKKVVVANILLQ
                     TSMTVRSDVRLEVGEVATSVRVEAAAPVINTEGAEVAAVTSNQVLEKMPMNVRGQFDG
                     FYYEMLTFIPGATRGQGSNFSLAGTRGSQFGATVDGTSQRSPLFGNSIGPAQSNMEMT
                     GEIRVQLANDKAEASLPGGVYATSRSGTNEYHGSLFWYHSNSRLAARNTFSSSVPFQI
                     QNNYGGSIGGPVVRNRTFFFTTYERFPLRNERIFNSSVPTAAFRNGDFSSLLPGTVIR
                     DPLTGTPFNGNMIPEERLSQSSLKIQERFFPVPNFGSANSYQQNWRGTGASSQYKTQV
                     EARLDHQLSAANSLFVRFSWNRTGANLWDYNLPTIPKRDQDRRSTTITISDNHLFGPT
                     LINEFRFGIMRTKNPAFNPLDGPALIQEFGLQGISWNPELDKGAPVFSFNNFEEIGAT
                     DIYQDPSERIHQVVNNVTWTRGNHVVKSGIDIRWNRGTNFPGGTSFPVLQFGQFTFAG
                     SYSGFDYSDFLLGIPQTAGRANAAPLIHALSTDMGLFVQDDWKITPKLTLNLGMRYEY
                     NPPTHEKDDNFFNFDVTTGRVIVPSEAALSRVNPLFPSNLVPVVTAEEAGVPRSLWYT
                     DLNNFVPRFGFAYRPFASNRTVIRGGYGIYTDDLTSSLWRLGTGGPFISRESFTNAIT
                     GGTPLFQFPNAFPPGFGAIGAQSFSAIDPKLRNPYIQQWNLTLEQEVFKMGVRLSYIG
                     TSTRQLVWVKNVNQVAPSLDRFDPSKRPFPALRDVTVRVNGGNHNYHSMHLVAERKML
                     NGLYYQLGWTWAKNMTNCQTDGDGGCTPLNPYNLNAEWGNVDYTNRHRVAGTLLFELP
                     IGRGKAFLSNLPRAADLAFGGWTLSSILVAQTGLFFSPTFSGFDVSNTNSTGTQRPDR
                     VGDGNFPSSERVITKWFDKTAFVVPGDVTGDGRPDVNVGRFGNSAPNVIEGPGALLLD
                     AGLHKQFRITERLRASLEGTFTNALNHPNYGSPNTNIRSGSVGRITSLYSRYGAGPRS
                     GRVGLRIEF"
     gene            complement(31964..32632)
                     /locus_tag="VLE22_20175"
     CDS             complement(31964..32632)
                     /locus_tag="VLE22_20175"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB16780.1"
                     /translation="MTSRLRVWTKWILLIATWAIAIVTLTKFSRAMWERYHSEERIEI
                     RGKRELTIIDREHKVRYEFAKQYCAGKTVADIASGTGYGMGILRTVAKSVEGYDREPL
                     GQKFIIDLEKQAWSEHYDVIVSFETIEHLANPEFFLGNVQKTAKMLLLSTPLDERKGW
                     ATNPYHKQVWTDASIKKLLEEFFRCEYYYQYGTRIEKDLPKNMRRLFVAVCTSPINSA
                     DVQP"
BASE COUNT         5668 a        10879 c         9793 g         6460 t
ORIGIN      
        1 agcggaaacc ggcggtcttc aaccagcacc acgtggagtc cgttgtccag cttcgtctca
       61 aagaccggcg gaagcttgaa agcgggcagc ggccccgtct cgggcggctt ggttcgatca
      121 atagtctggg cggaaaggtt catggcggca atcactgcaa atgcggtcag cacggccttg
      181 gtcatggtcg cctacttccc cgtcttctgg gcgctgggtt cggctttttc ggccggcggc
      241 gccggcacga tttccagcgt gaatcgtttc tcgggcacgc agtacttccg cgccgcggcc
      301 tggatctgct cgggcgtcac cgagaggaac gcgtccatct cggaattgat gtagtccggg
      361 tccccgtcca gcagctcgta ctggccaagg agcatggcgc ggcggagcga gctttgcagc
      421 ccgctaatgc gcgaggagcg gaggaacgtg cgcacgcgcg cgagttcctt cgccggtacg
      481 ccgtcctttt ggatggcagc gatttcctcc tgcacctggc cggcgatctc cttgccggag
      541 aacttcggat tgtgcagcag cagcatcgcg taatgaccgg ggtgcttgta gtcgctcgac
      601 gcggcgaagg gccagccgag atttgcctcg aactgaacca ctgaccgttt gcctttgacc
      661 agattctgct ggaaacggga actctcgccg ccggtcagga ccacgtccag catcgccagc
      721 gcatagaact ccgcggaccg gcgctctggt cccgggtatc ccaccactac cgccggcacc
      781 tgcgccagcg ggtcgcggta cacctcggat tgcgcctgca cctcggatgg ctcgctcagg
      841 tcgggatgtt tcggctgcgg ctgcgcgggg atgtctccga agtacgtttc gacgagcttc
      901 ttcgctgccg gaatgtcaat gtccccggaa agcacgagca cggcgttgtt gggcgcgtag
      961 tagaccttga agaaccgggc tacgtcgtcc accgtggccg cgttcaagtc ctcgaaggaa
     1021 ccgatcaggg aatgcgaatt gctccaattt cggaatgcga tcagcggcca gcggtcgacg
     1081 atggcggtgg catagggctg gttgtcgaac gacagccgcc gctcttgctt cactgcctcc
     1141 ttctggtttg tgaggttgtc gtcggtgatg gcgaggctgc gcatgcggtc cgattcgagc
     1201 cacagcccca ccgccagttt gttcgccggc aacacttcga aatagtcagt gaaatcggga
     1261 tgcgtggagc cgttcaacgt tcctccgttg gactcgacct gtttgaaatg ctgtcccttg
     1321 cccgcattcg ccgagccctg gaacatcatg tgttcgaaca ggtgggcgaa gcccgtgcgg
     1381 cccttctctt cggacctggc gcccacgccg tagatcacgt agacgctcac cacaggcacc
     1441 gccttgtcgg gcgcgaggat cactcgcatg ccattcttca actggtattt ctcgaagggg
     1501 attcggaaat cggaaccact aaccacggcg gctccagcgc agagcacaag catcgcgaat
     1561 attgacctca tgaagctcct tgattctgga acggctccgc tcgccggcgc ctcttggtgc
     1621 cgccggcagg ccctcacttc ttacgcaaaa accgggtgaa ctgttccagg gaacgtgtat
     1681 tcatgatatc atccgccgcc agccagccgc gccgcgccgt cagcacgccg tacttcatat
     1741 tcgtaagatg cttcgggtga tgtgcgtccg ttgagatcgt gaagcgtact ccttttgccc
     1801 gtgcggagcg aagcagtgga gcgtcgaggt cgagccgctc gggactggcg ttgatctcga
     1861 agcaaacacc gcgtcgggcc gcttcggcgg ctacgcgctc gaaatcgaac ggaaatgcgt
     1921 cgcggtggag cagaacgcgc ccggtcggat gcccgagaac tcgaaggtga gggctttcaa
     1981 gcgcccgtag gagccggtct gtcatctcgg ccgattcgag gctcatgtag ctgtggacgc
     2041 tgccgataac caggtcgagt tcggccagcg catcgccggc cagatccatc gacccatcgc
     2101 gccggatgtc gcactcgagg cccgaaaaca cacgaatccc gaggccatcc ttgttcaact
     2161 cccgaacctg ccgggcgaac gacaccgcgc gtttctcatc gagcccgttg gccattgcca
     2221 gcgctttcga gtggtcggtg atggcgatgt acttgtaccc gagctcccgc gccgcctggg
     2281 ccatctcttc gagcgtcgcc cgcccgtcgc tctcgatcgt atgcatgtgc aggtcgccgc
     2341 gaatgtcccc gagttccacc aggttgggga gcctgccctc ttcggcggcg tcgatctcgc
     2401 cgaggttttc ccgaagctcg ggaggaatcc atgcgagccc gagcgcgcgg tagacatcct
     2461 cttccgtctc gccggccact ctcgattcgt catcggtgcg gaacaggccg tactcgctca
     2521 gcttgaagcc catacgcacg cctcgagtgc gcagggcgac gttgtgctcc ttgctgcccg
     2581 tgaaatactg aagcgcggcc ccatagctct cccgcggaag cgcgcgaacg tctacctgca
     2641 ttccttcgag tccgaacttg gcgcttgcct tgttgccgcc tttgccgagc acctcgctca
     2701 ctttcggatg tttgatgaag cgctcgatcg tggcggtggc gcccggcccc gttacgagca
     2761 ggtcgagatc gcccaccgtc tccttcccgc ggcggagact gcccgcgggc gtcacctgtt
     2821 cgacacccgg ttccgacgac agatactcgc tgagttcccg cgccagttcg tccgcaaagc
     2881 tcagcagaaa gcgtccgacg cgcttgcggt actgggctat cgaacgcaaa accttttctt
     2941 cgagcttcgc gcccatcctc ggcagcaccc ggagcttctg ttcgagacac aggcgctcca
     3001 ggtcgtcgat tgtgctcacc cggtagtgct cccagatcag ggcgatgctt ttcggaccca
     3061 ggccttggat cttcagaaac tccagggccg tcggcgggta ctttgccaga agctcgtcgc
     3121 ggcgctcgaa cgaaccccgc tcggcgatct cggccagcac cgcggcaagg ccttttccga
     3181 tgcctggaat ctccgttacc gaccggtccg ggtccttgag gatgtcgacg attcgctccg
     3241 gatggttctc aatcgccgac gcgccgttcc ggtaactgcg gatgcgaaac gagtcctctg
     3301 cggcgatctc catcaggtcg gccgtttccg cgagaagacg cgcgatttcc ctgttttcca
     3361 tcgctggagt gccccggaaa aattgtagcg ctttcggccc gtcagctcgg tctatgggcc
     3421 tcttgccctt cggcgaccgg tgcctcgttg agcagtggag ggtagggctc gacgtgcacc
     3481 atgacgtctg ccacccacgg aagcactttg gtgatccgga tccggacctg tgtcgcgatg
     3541 tcgtgggatg agcgaacggt catctgtggg tcgacttcga gatgaaggtc gacgtggtgc
     3601 tgaagcccgg tcttccgcgc gtaacatttc tccacgccga gcacacccgg taccgaaagc
     3661 gcggtgctcc ggatctggcg cataaggtcc ggctccggca tcgtgtccat cagttgcatg
     3721 gtcgtttccc ggacaacgcg cacaccggtg aaaatcacaa tcagcccgac ggcgaagcct
     3781 ccgtaatggt cggcggcgag aaatcgcccc ggattgtaaa gtgtcagtcc gacggctgca
     3841 agcgccgtgg agcccgaaag gatgtccacc atgtcattcc agccgtcggc cgtcagagac
     3901 gcactgtgga tctttcgccc atagtagaac ttcagacggg acatcaccgt cttggccgcg
     3961 atggacgcca ggagcggcca aatggcatac gccgcgggcg ctgtgtgcac aaagttcact
     4021 cgttcgagag agtggattgc gatgccggtg cctgtcgcga cgagtaccag gccgacaagc
     4081 agaccggtga gagtctcgaa gcgcccgtgg ccgtatggat gatttgcgtc gggtggtttg
     4141 gcggcgacaa gcaacccgaa caggacgatc gccgaggtgc ccatatcccc tgccgattcg
     4201 acgccatcgg caaacaccga ggtcgagttg accagcagcc caaccacgat cttgctcgcg
     4261 gccagtacgc cgctgaccgc catgctggcg atagcaaccc gacggccggt gcgctgcaag
     4321 tcgcccatga aggcaaggat accagccgta cgctgcgcct cgcttgcggg atcgcgcgcg
     4381 aggctgatat cctcgactcc acctgggaga ttcgtatatg acactaacag acgtgcgaaa
     4441 actggccatc cgccggcagg cgcgcatccg cttctctgtg tccgccggca ccgagtgcgt
     4501 gatcgacgag cacggcgtcg ttcgcgtcgc cggtctcgac ggaccgccct ccttcagcct
     4561 ggaagaggga ttctcgggag ccggccagtt cgtcttggag ccggtaccgg ccgctggcgt
     4621 caagaatgcc cccctgcggt cgctctgccg tgacgagctt gaggtgcttc tttccgatgt
     4681 cactcccgcc gcacaaaaaa ccagcgacga cgagggctag gcgcctatcg ggcgagaaaa
     4741 taagtattcc attaagctcc gaattgagag ataatgccta gcaagatttc gcgtggagta
     4801 agcacaatgc ggctgtcatt tcgtatcgtt ttcgtgcttt gcttcttcac attcctgctt
     4861 ccggcgcaga ttgaagtttc caccagcacg ctctcattcg aagcgatcgc tgggggcagt
     4921 cctccgccgg agcagcgcgt ttacgtgatc gcctctccgg gaggcacgct caccgaggtg
     4981 gcgctcgcgg ccgacggggg cgctcccggg cttctcgccc cgaaatggct tgtggtcgct
     5041 ccgaaatcgc tgacgatgcc cggagcggtc cgcgttgcgg tcgatccgtc gactctcgtg
     5101 gccggcacgc aagcgtctgc gagaattctt ttcacgaaga tcgacggtac ggcgctggga
     5161 ccgcaggtcc aggtgacgct gaatgttcga gagagcgccc ccaagcttgt cgtgacgccg
     5221 gaaaccctgc atgtttacgg caaagcgacg acctcggcgt tcgtcgaaag tgacatcgta
     5281 cttcgcaata ccggtggcgg cagcctcgcg ccggtgacgg ccgaggtgaa aagcgccgcg
     5341 ccgtggatga gcgtcaaggt gctggagccc tgcgccgtcg aatgcacggt tcgcgtcacc
     5401 acccgttatg ggatggaaac cggaacgcgc tttgccgttg tcgggctcaa taccgccgcg
     5461 ggcgccgctc gcctgcccgt cgcgcttcac gtctccggcg acgggtccat cctgagcctg
     5521 gaaccgccca cgattgcgct cgaggcgcgc tcggggcacg gtctcatccg atcgcgatac
     5581 tctctcgttt actccaacgg cgacacgccc accgactgga tcgtggagaa aatcgccggg
     5641 ggggcgtggc tgactctgga cagcacaatt ggagtgtcca gccgttcgat tcccggaacg
     5701 gtgtccgttt ccgcgaattc agccgctctc gtgcccggta cctattactc cctgctccgc
     5761 ctttcctcca gcggcgcgct gaactcgccc ttgtatctgc ccgttgtcct gcgtattgcg
     5821 gagactgctg agccgccaat cccgctcctg cccctcggcg gctatgtgtt caccgcgaag
     5881 attggggaga acccgccgcc aggtctgaag ttcaatctgt cggcgagcag cgccacgccg
     5941 gtgcaatttc gcgcttcggt ggatcacgtg accggcgccg gctggctgac ggtaagcccg
     6001 acttccggct cggtctcaag caccgccgcc gctagcatca atgtttcgac caccgtgctg
     6061 ccgaagggag tgtacacggg aaagatccac ttctcggtcg ggaatctggg aatccgttcc
     6121 ttgaacgtaa ccacgatttc gtctccagcc tctggctgcg ttcccggctc ggtgcacatc
     6181 acgcacctcg ggatccccga cgatttcaag gcggccgtag gcgctcatct cccgctcgtc
     6241 gtgtcggtgt tgaacgactg cggtgaggcc gtgacgaacg cggcggtgac ggtggagttc
     6301 tccaacggtg agcggtccct tccgctgcgg cacatcgcca acggtgttta cgcggaagcc
     6361 tggagcccct cgaaagaagc agcggcgctc aacatcacgg ccgtggcgac ctcgtctgcg
     6421 tatccattcg tttcccgtgc catggtggcc gggagtgtaa cctccggctc cggtcctgtt
     6481 ctgttcgcca acgccgttct gaacaacctt aacccgctgg tcggcgcacc gctggccccc
     6541 ggaaccgtgg ctcaggtcta cggaaagaac ctcgccaccg ctaccgtcca gccgggtttc
     6601 gtgaacggca aactgccgtc aaccttcaac gagacatccg tgcttgttgg ggacataccg
     6661 gcgccgctct actacctgag tgacagccag ctcaacgtcc agcttccggt ggaactggcg
     6721 tccggtcggg agtaccaggc gctcgcagcc cttaacggag cgtggtcggt ccccgtcacg
     6781 gttttcacgg agcccgcaac tcccggcgtc gtcgtctact ccgacagcgg agtcatcgcc
     6841 cagcacgccg attacagcct cgtgaacgcg gcgagtcctg cccgccccgc agagtacatt
     6901 cgcgtatacc tggtcgggat gggttccacc aaccctgtgg tagcaacagg actgcccgcc
     6961 ccgccccagc ttcaccgcgt ggtcaaacag ccggatgtca ctcttgacgg cgctccagtc
     7021 acggtctcgt acgcgggcct ggcccctggt tgggtcggtc tctatcagat cgacttccag
     7081 gtacccgcat cggcccaaac tggtaacctg aagttgacca ttactcagaa cggcgtactg
     7141 gccaacgact cgtacctgcc cgtccgccgc tgacagctga tagctgaaag ctgttttagt
     7201 gctacaatct ggagacaccc ggcagcagcc tgttgagggg gtgcagttcc cgaacgaggg
     7261 agattcgatg gagaagctgg cgcaaaacat tcaagaagca tttttaaaca acgcgcgcaa
     7321 agagaagacc ttcctcacca tctacctgat gagcggcgtg aagctttccg gccgcatcaa
     7381 gagttttgac aaatactctg tggttttgga gacgaataac caggagcagt tgatcttcaa
     7441 gcacgcgatc tcgactttgg tggtttccaa accgacccac acctacgcgg cggtcacgca
     7501 cgcacaagct ggtcccccgg tggtcggcgg tcctcacccc gctccgggcg tgccctccca
     7561 cgagtccacg gaggagtaga ttacccggct ggtgaaggag tctgacgagc gggccatcct
     7621 ggtagggttg gagctgcgcg gacggactgg gtcgctcgcg gcgggcaagg cggagtcttt
     7681 ccggtattcg tccgaagagt ccctggaaga gcttgcggcg ctggcggaga gcgccggagc
     7741 tgctgtagtc gagcacgtca tccaatcgcg ccccgctccg gaagctgcca ctctgatagg
     7801 cagtggcaag gtgcgggaac tcgcgcagct caccggggcc tcgcaagccg acgtcgttat
     7861 cttcgacagg gatctcagtc ccactcagca gcgcaacctg gagcgtgcgc tctcggtcaa
     7921 ggtggtcgac cgcacccagc tcattctgga cattttcgcc cggcgtgccc gcactcggga
     7981 gggccagctt caggtcgagc tcgcccaact gagttacctg ctgccgcgcc tggtcggccg
     8041 cggtgttgag atgtcgcgcc tcggcggcgg aatcggtacg cgtggacccg gcgagaccca
     8101 acttgagacc gaccgccggc ggatcgcccg gcgtctcaag aagatacgcg tggaactcga
     8161 aagtgtgcgc ggccatcgct cggttcagcg caagcagcgc caggcgatcc cgctcgccac
     8221 tgccgccctg gtcgggtaca ccaacgcggg taagtccact ctcttcaatc gcctcacggg
     8281 agccggcgtc ctggccgacg accggctctt cgccacgctc gaccccacga ttcgtgccgc
     8341 aattctgccc tcccgccggc ggattctgct gagcgacacc gtcggattca ttcgcaacct
     8401 gcctaccacg ctgatcgacg ccttccgcgc cacgctggag gaggtgatcg aggcgtccct
     8461 cttgatccac gtcgtcgacg cgtcctccgc gcacgcggta caccagacct cccacgtccg
     8521 caaggtgttg accgagatcg gcatcgccaa cacaccgcag gtcctcgttc tcaataaagc
     8581 ggacagcctg cccgccggcg gcatcgaccg ggaaaccgtc catcgccgcc tggccgagga
     8641 tctcggagcc atcgttcagg cccgtgccgt cagcatttcc gcgctcaccg gagcgggtat
     8701 cgaacgcctc ctggaggcaa tcgacgaaac gctgccgctc gatcctgtcg agcgcgtgca
     8761 cttccggctc ccggcaggag agggcgccgc gcttcatctc ctccacgagt tcggccaggt
     8821 gcgcgccgcc gactacgcgg atggctactg taatgtcgaa gcggacgtgc ctgagtccat
     8881 ccggcgccgg cttactcgct acctggtaga aagttaatct gtggaaaacg ttgtgggaat
     8941 cctgcgggaa aggactcccg cgtcgcatgg attccgctcg cgatcttgtg caaggcatta
     9001 tttttcgtgt agatgccgcc ccggcacctt agggagcggc caaactaccc cagttacgtt
     9061 ttccacaagt tggcgcaaaa caccaagccg ttctatattt gtttagggtc gatgaatcta
     9121 ccgaacctgt tgaccattct gcggatcttc ttcgtgccgt ttctggtcgc agtgttggtg
     9181 cagggaaagc tcggtatcga cgtcggtggc tttcacctct cgaacgacat tctggcgctc
     9241 ggtatttttc tcgccgcggc cgcaacagac ctccttgacg gatatctcgc tcgaaaatgg
     9301 ggccaggtga cgacggtcgg tatgctgctc gatccgatcg cagacaagct cctgatctcc
     9361 gcggcgttga ttgcgctcgt ccagatacgg ctgataccgg cgtggatggt gatcctaatc
     9421 gtcggcaggg agttcgccat ctccggactg cgaagcattg ccgcggctga gggctacacg
     9481 atccgcgcca gcgatctggg caagaccaag atgatgtcgc aggtcatcgc gatctccctg
     9541 gtcgtgctga gcatccgctg gcgccaactg gctccgtacg ccaatttgtg gctctgggga
     9601 gttgtcgtat tctcgcttgc atccgccgtg cagtacttcc gaaaattctg gagaagggtg
     9661 gatgaccaga tcaagctgcg gggccgccgc gaattgcttc tgctcgaacg gctggcgaag
     9721 cgagagcagc gcaaacggga acgggcagcc gcggcggaac gtgcggccgg ccaagggctg
     9781 tagcgctcag ggtctataat aaacggttcg gtgaatcacg aggcagacgc ggaaatcgct
     9841 gtcattgtcg tcaactggaa ccgccgcgag cttcttcgcc gctgcctgga atcgctggcc
     9901 cggcaggaag gcgtcgactt cgagatcatc gttgttgaca acggttcggt cgacggttcg
     9961 ccggacatgg tgaaacgcca gtttggcgcc ggccctgttc cgttgcgttt gatagaaaac
    10021 gcggaaaacc gcgggttttg cggggcgaat aaccagggca tctgcgccac ccgaagccgt
    10081 ttcgtggcgc tcctgaataa cgatgccgaa gcggcggcag gttggctggc cgccctgagg
    10141 cggtgcttcg acggccggcc cgatgtcggc atggtcgcct cgaaaatcct cgtgttcgag
    10201 gatccgcggc ggatcgacaa agtcggtcac ctgatctacc cggacggcca gaacagaggc
    10261 cgtggaaccg gagagttcga caccggccag tatgaccgta tcgaggagac gttgtggccg
    10321 gatggatgcg ccgcgatgta ccggcgcgag atgctggatg agatcggcgg attcgatgag
    10381 gatttctttg cttatgcgga cgacgccgaa ctgggcctcc gcgcccggat cgccggctgg
    10441 aattgtctct acgtgcctga cgccgtcgtt cggcatcacc gcggcgctac tctgggtgtc
    10501 gcctccagcc gtcgtctcga actcatcgag cgcaatcgca tccttcttgc tgcgaaactg
    10561 tttccttgga gcctgctatg gctgaacggc ctgtactaca cggcgaggat cggagccgga
    10621 atggtggccg ccgctcgggg gaaaggcgaa atcggcaggt ttccgggaat ccgcgagaaa
    10681 ctgcgggcgg caatggcgtt gttgcggggc gacctgcagg cgcttcggat gcttccgcgg
    10741 atgctccgga agcgccgcga gatcgcgaag ttccgccgcc tctcctcctc ccagatccgc
    10801 cggttgatcc tccaccaccg gatcccgctc cgcaagttga cagaggaatc gacctgaagg
    10861 ctcccgagcg cgcatcttcc ggtggcgagt gccccgcctg ccagtgcggc gaattcctga
    10921 gcctgttcac tgccacggac cggctttacc ggaccactca caacaggttt caagtcgttg
    10981 agtgcgcacg gtgccgtttg atccgcctcc atccgcgtcc aacacccgaa gaaattgcgc
    11041 agtattatcc ggcagactac tggttcgcgc cggatgacgc cgccgccgac tggctcgaag
    11101 agatctaccg ccgcttcgtg ctccgggatc atgtccgctt cgtcaggcga gcggtggaaa
    11161 gctctcgcgt gcaaggcccg gttctcgatg tcggttgcgg cggcggactt tttctccgca
    11221 tgctggctga acgtggtttg cctgttgtcg ggctggactt ctcgctcgac gctgcggtgc
    11281 tttcctggcg cgtgaacgga gtgccggccg tctgcgcgac cctctctcag gcgcccttcc
    11341 cttcggaaag ctgcgcggcc gtcaccatgt ttcacgtgct cgagcacctg tacgatccgg
    11401 tgtcatacat cgaggccgcg catcgcctcc tgaaacccgg cggacggttg attgtccagg
    11461 tgccgaacgc cgcttgctgg cagttcctgc tgttgggtga gaactggacc ggagtcgacg
    11521 tcccccgcca tctgtatgac ttccgggcca gcgacctcga ggtcctcctc agcgactgcg
    11581 gctttgatgt ggtccgccag aagtacttct ccctgcgcga caatccggcg ggcatggcca
    11641 ctagtctcgc cccgtcgctg gatccgatgg tccggcgggt gcgccgcaca caggagacgt
    11701 ctcgaatcag gctcttcaag gacctgatct acttcggctt ggtcgtctgc tgccttccgt
    11761 tcaccctgat cgagggcgct tgccgtgcgg gctccacggt aatggtggag gcgcgcaaga
    11821 aagcatgaac cgcgccgcga ttctgcgctg cctgcccgga ccgttgcgcc gtcacatcct
    11881 cttcttcgag gctgagatcg aacgcgcggt cgccgtcttc gcccgatctc tcgatcccgg
    11941 ctcccgtgtc cttgacgccg gtgcgggcga agggcgctat gccggcttct tcccgaacca
    12001 acggtactgc ggcgtcgacc ttggcatcgg tgacgccgcg tggaactaca gggggctcga
    12061 cgtcgtcgcc gacctggccg ctcttccctt ccccaacggc tgcttcgacg cctgtctgaa
    12121 catcgtgacg ctcgagcacc tgccggaccc cggcgcggcg ctggtggaaa tcgcccggac
    12181 tctgaagccc ggtggccggg tcctgctcgc cgttccccat gaatgggagg tccaccaggc
    12241 gccacacgac tatttccggt tcacccgcca cggaatctca catctgttgg aaagggcagg
    12301 attttcgtcc gtcgatgtcg agcccgccgg cggtttcttc cggctcctct cgcgaagact
    12361 gctgaatgga ttgcagttct ttcctccact gctcgtaccc gtggccgccc tattcctcgc
    12421 gccgccggcg ctcctcctgc ccggtttcga cttcctggac cgcgaacgca atttcacctt
    12481 ggggtatcta tgcaccgccg tcaggcgctc ttaacccttc tccgcgccct cgcgccccct
    12541 gcgggcttca acggcgactc ggcgctgcgg tacaccgtga aggcggtctc ctttggtccc
    12601 cgtcctccgg gatcggacgc gatccaccgc ctgcaggcct acatactctc gcagttgaaa
    12661 acgacaggct gcgagatcat cgaggatgac ttcactgccc gaacgcggtt cggtccggtc
    12721 gcgatgaaga acatcatcgc ccggttccgc ggtacgtcgg gccgcgcgct ggccatcacc
    12781 gggcactatg acaccaagct catgcgcggc gtccctttcg tcggagccaa cgatggcggc
    12841 tcctccaccg gattcctgct cgagatggcc cgcgtgctcg ccggcaggtc gaacgaggac
    12901 gacgtgtacc tcgtctggtt cgatggggaa gaagcgctgg tcgcatggac cgaggccgac
    12961 ggtctctacg gaagccgcca cctcgcgcgc cgatggtctg acgaccgaac agccacacgc
    13021 ctcaaggcgc tgatcaacgt cgacatgatc ggcgaccgtg acctcggcat tcttcaggag
    13081 atgggctctt ctccgggctt gcggaagctc gtctggcagg tggccgaaga gatcgggtac
    13141 ggacggtact tcctcgacgt ctccggtaat atcgaggacg accatgcccc gttcgctgcg
    13201 gcaggtgtca atgcgctcga cctgatcgac ttcgactacg gtcccggaaa ctcgtattgg
    13261 cacaccgccc aggacaccgt ggacaagctc ggcgcccaca gcttccaggt ggtcggtgac
    13321 gtcctcttgg aagtgatccg ccgtctcgaa cgatagaggg tgctgaagag tagcacaggc
    13381 ctcagcctgt ggatcgtgtt ttgcatcaac cgcttattcg ggctacgtct ccggttcatc
    13441 cttcagcgct gggtcggcgg tatcgaattt cggaccatcc gcgtcccagt cttcagcccg
    13501 gaacatgcgg ccctggcccg ccggtccacc gatctctttg attgttactt gcaccttcgt
    13561 gcctttcacc cgcacccacg cgaagtgata gaacgctccc tgttcgaagc cctgatccgg
    13621 tgtcaaatcc cgccggaagt gcgcaccgga gctgcccaat gcgacgtacc cgatcccgtc
    13681 acgctccatc cgcatgaact ggtgtccatg cccgctgatg atctggtcga cgccatactg
    13741 tcgcgccagt cggtgcaacg ggaactctcc gctgcctgcc ttcagaaaaa ccacccagta
    13801 aggcttgtga aagaaaatga acttcggccg gcggtgcctg ttccggcgca ggtctttctc
    13861 gagaaacttc aactgctcct cgtcgaggtc cagcgtccga ctgttgtcga gcacggtgaa
    13921 gtgtgcgtcc tggacgttga agctatagaa cgatcgccgg cccgtttcgc gctcgaacag
    13981 ctcccgcgac ttgtcgctcc agatgtcgtg gttcccgggc gtgaagtaga acgggaacga
    14041 agcgtatctc cgcaaaatcg ctctgagttc ctgccattcc gtctcggcgc gcgtgtcgtc
    14101 gcctccctgg atcgtgtcgc cgatgttgat ggcgaaatcc ggagatagca tcgccacctc
    14161 ccgccagacg cggccataaa tttgcggcat cgccgagccg gtccggtcgc cgaggatggc
    14221 gaagtggaag tcgttgcccc ctcgcgaaaa cgccggcccg ctgaagaaag cggttacggc
    14281 aagcgcaaaa agtactcccg caccccagaa tcgaagcttc cgtcgcatcc gttcattctg
    14341 ccacggcacg gatgggcgga cctccgggcc ggcctcctcc agggttcttc taggattctc
    14401 tgtgccctct gtggtgaagc cagctccaaa tcgcgcgcga gttttccggc ctgtggccca
    14461 cttatctact cgggggaatt cccctagcag ggtgccgaaa caaccgccgg cgttgtcgct
    14521 cgacacagcc aaaggccgtg ccaccgctga gaatcaactt cttacggcct gcggtggcac
    14581 aggcttcagc ctgtggcgct ggtttttcag caccctgata gccggtatcc atgcatacgc
    14641 tcgactttgc cgcggctggc ttgctcatcc cgctggcaat cctcatgctc gtcagcggcc
    14701 tggacgacct tctgttggtc ctggcgatgg catggcggtg ggtccgcctc cgccggctcc
    14761 cgcaaggtcc ctcggacgcc gacctccaac tcgtgcccga gcagcgcatc gccatcttcg
    14821 tgcccctctg gcgcgaggac cgcgtcatcc ggcgcatgct cgaacacaat ctcgcagcca
    14881 tccgctaccg ccggtacgac ttctttgtcg gcgcctaccc caacgacgag ccgacccttg
    14941 acgccgtgcg caatgccgag cagcgttacc caaacgtgca cctcgccgtc tgcccgcacg
    15001 atggcccgac gtccaaggca gactgcctca actggatcta tcagcagatg ctggtgttcg
    15061 aggacctccg cggcgtctat ttcgacgtca tcgttaccca cgatgccgaa gacctgattc
    15121 atcccgactc attgcgttgg atcaactacc actcaagctc ctgcgacatg gtgcaggttc
    15181 cggtgcttcc gctcgccacg ccgttgcgca gcgtcaccca tggcgtctac tgtgacgagt
    15241 tcgccgagta tcagaccaag gatgtgccgc tccgtaccgt tttcggaggc tttctgccgt
    15301 caaatggcgt cggtaccggc tatacccgcc gcgccgtctg gaaactggcc gaaatgtcgt
    15361 ccaaccgcat tttcgagccg ggctgtctca ccgaggacta cgacaccggc ctgcggctgc
    15421 accgccttgg attccggcaa atgtttgttc cgctccgctt cgccggaggg cgcccgctcg
    15481 cgacccggga gtacttcccg caatccttcc gccaggcggt tcgccagcgg acacggtggg
    15541 tcatcggcat cgccctgcaa tcgtgggagc ggcacggctg gaaaggcggc ccgtcggtgg
    15601 cctactggtt ctggcgcgac cggaaaggcc tactcggcca tccggtcacg atcgctggca
    15661 acgctctgtt cgcctatggg ctggcgtcat ggctctggag ccgcgtaacg ggtttgccgt
    15721 ggaatcttag ccaccacgtc gctcaaccgc tgttcacgcg attgctcgtg gccaccacgt
    15781 cgctccaggc cctgcacatg cttgtgcgcg cggcctgctc cgctagaatt tacggctggc
    15841 gtttcgcgct cggcgtcccc gttcgaacgg tgtgggcgaa ctggatcaat tcggtcgcca
    15901 cgacgcttgc tgtctgccgc tacgtatggg cccgcttgga acgccgcccg ctcgtgtggt
    15961 tgaagaccga ccacctatac ccctcacgcg aggctctccg gcagcatcgt cctcccgagc
    16021 ccgccgtatg gacctccgat cacgtcgacg tcggctcgat ccctcccgcc ggcgctcaag
    16081 tccggtagct cgcgttgatg cggatgtacc cttccgtcag gtcgcaggtc cagaagcagg
    16141 tctcacccct cccctggccc cggatcgcaa agcggatcac gcagtccgag gtatcgagtt
    16201 tcttcttcag ttcgagctcg gagaactccg ccgcaaggcc cctgcggcac accggcactc
    16261 cctgaagcag gatgtccacc ttgtcgggat cgaactccac accggaagct cctgccgccg
    16321 acagaatgcg cccccagttc ggatcggagc ccgcgatcgc cgtcttcacc agcggtgagt
    16381 tggcgatcgc cctcgcgatc tgcgtggccg ccttgtcgtc cttcgccccc accacgtgga
    16441 cggtcacctt cttgtttgcg ccttcgccgt cccgcgcaat catttccgcg aggtcctgca
    16501 tcacccagca gagcacttct tcgaacacca tccgctcctt gctggtcggc cgcaagcccg
    16561 atgccccgtt tgccagcaga atcagcgtgt cgttcgtcga catatcgcca tcgaccgaca
    16621 ggcggttgaa actccgctcc gtcgagtgcc gcagcatcct cgcgagggga cccggttcga
    16681 tctccgcgtc tgtcatcacg aatccgagtg tcgtcgccat ccgcgggtgg atcatccccg
    16741 cccctttggt catgccggcg atgcgcgcca tgccgtggtt gaacggcacc tcgccgaagg
    16801 cgaccttcgg gatgcgatcc gtcgtcatga tcgcccgcga cacgtcctcg aagcgtgttt
    16861 ccgaaaggcc cttgaacagc gcgggcagcg agccggtcac gagagaggcg tccatctcga
    16921 caccgatcac ccccgtcgac gccggcagaa cttgcgtcat cggcgacttc agttccttgg
    16981 cggccgcgcg gcagcacgcc gccgccaccc gcatcccggt gcgggtcgcg cagttcgcgt
    17041 tcccggcatt cgctagtatc gcccgggctt ctccacccgt ggccttcagg tggtttcgtg
    17101 ccagccgcac gggcgcagcc tgcacacgat tggtcgtgaa gacggcggcg gccgaagccg
    17161 gcgcgtcgct ggcgatgatg gcaaggtcgt cccgcttgtc cttgcgaatc cctgcgtatg
    17221 tggacgaata tcgatagccg agcggcaatt tcactggctc aaatccccta acatcccatg
    17281 tctcgtgggg cagacaatcg ccgtttgtcg tctgccgcac tcacaggcca tggaaaacga
    17341 tggcctgcgc cgcactacag taacctctcg ttctccgcca ggcgctgccc gttgacgaac
    17401 gcctccgaag tcaccctttt tcttccttcc aactggagct cgatcacctc caggctggtg
    17461 ccctgcccgc acgccaccag cagccgacgt ctggccggct tgagtgtccc gggcgcttcg
    17521 gacaccgcgg catcgccggt cgtcgctctc cacacatgca acaactgtcc gcgaaaaacg
    17581 ctccaggcgc ccggccacgg aacaaaaccg cgcaccctat tgtagatgtc cgcggcgggc
    17641 aggttccagt caatccggcc gtcttccttt cgaaggatcg gcgcgagtgt ggcgagtgac
    17701 ggatcctgct tccgcggaac gacgctgccg gcttcgagat cgcgaagcgt ttcctccagc
    17761 agttccgcgc ccatcgagga caaccgcctg ccaagctcct ccgccgtctc ctccggcccg
    17821 atcgccgttt cccgttggag caggatgtcg cccgtgtcca ggcccgcgtc gatccgcatc
    17881 gtggtcacgc cggtgaccgg ttccccgtgc gagatggccc actggacagg cgccgctccg
    17941 cggtatttcg gaagcagcga cgcgtgaaca ttgatgattc cttgtggtgg aatgtcgatg
    18001 atgttctgcg ggatgatctg gccgtagccc accaccacca tcgcgtccgg cttcagcgcg
    18061 gcgagcgcat ctacgatttc cggacgccgg atcctctcgg gctgataaac cggcaagccg
    18121 tgacgtaacg cagcgatctt cactgcggac gccgcgattt ctccgccccg gcccttcggt
    18181 cggtcaggct gcgtgtagac ggcggaaact tcgtggccag cttcaatcag ccgttccagc
    18241 gtgggaaccg cgaagcccgg agtgccgaga aatacgaccc gcattttctt gaaccgcgac
    18301 cgtcggcgag cggtccctgg caggacgctg aaaaacccgc cggcacggac gcccgacaca
    18361 gcctaaaggc tgtgccacca ctgagaatca gcagtttacg gcctgcggtg gcacaggctt
    18421 cagcctgtgg cgcgggtttt tcagcgccct gctagtcccc gccgagccgc cctcagctcc
    18481 actcgcccgc cttgatcatc tttcggacct tgcgccggat caggtcacgc ttcagcacgc
    18541 tgacatggct caggtagagc ttgccgttca ggtggtccgt ctcatgcaaa aacgctcggg
    18601 ccaggacctc ctcgccggtc atttcaaacg tctctccctt cgcgttctgc gcgcgcaccg
    18661 tcacccgtag cggccgcgtg acctcctccc ggaaaccagg cacgctgaga catccctcct
    18721 cggacgtctg cttcccttcc ttgcggacga tctccggatt gatgagcacg agcttctgcg
    18781 atccgtcctc cccaaccgac gtgtcgatca ccgcaatccg tcgcgagatg ccgatctgcg
    18841 gcgcggccag tccgaccccc ttcgcagcgt acatcgactc gaacatgtct tcgatcagtt
    18901 tgtgcaagtc gggtgtatcg aactcgtgga ccgtctcggc tggtttctcc agaaccgggt
    18961 cgccgagctt gacgatgggg taaatcatca gaatcgcctc acctgctcca tgtagccgtc
    19021 aaaattccgc ttcgcttcgc gcatcgagtc gccgccgaac ttttcaagca tcgcctgcgc
    19081 cagcaccagg gccgtcattg cttcgccgat cactcccgcc gccggaacga cgcagacatc
    19141 gctccgctcg tacgcggcct gcacagcctc ccgcgtgatc aggtccaccg actcgagcgg
    19201 ccgccgcagc gtggaaatcg gcttgagaaa gccccgtaca acgacgtcct gcccgttcgt
    19261 gatgcctccc tccagaccgc ccgcccggtt cgcgccccgc gcaaaacgcc gcgactccag
    19321 gtcgtagtga atcgtgtcct gcaccttcga gccgaagctg gccgcgttct cttccgctgg
    19381 accgatctcg actcccttca ccgcctggat ggaaacgata gcctgcgcca gccgcccgtc
    19441 cagacgcgag tcccaactga tgtgcgatcc gaggcctggc ggcacgttgt gcgccaccac
    19501 ttcgaagatc ccgcccacgg tgtctccggt gcggtaagcc tggtcgacga cctccttcat
    19561 attcgcttcg gcctcggcat cgacgcagcc gagcagaacc tctttcgacc ggctcaatgc
    19621 aaccagttcc tgccacgtcg ccggccggcc gaggcagacg ccgccgacgg ccaccacgtg
    19681 actgagcacc tcgatgccga actcgcgcag gtacgacttc gctatggcgc cgagggctac
    19741 ccgcgccgtc gtctcgcgcg cgctggcgcg ttcgagcacg tagcgggcgt cgtggaagtc
    19801 gtatttcaga acaccggcga ggtcggcgtg cccgggccgc ggtcgcgtca ccactttgcg
    19861 cttttcctcg ccgccgtcgg tgtcctccac cgccagcgcg tcgcgccagt tttcccaatc
    19921 cttattcttg atcagcatcg ccactggcga gccgatcgtg tacgagtgcc ggacgccggc
    19981 gacgatttcg acgtggtccg tctcgatctt catccggccg ccgcgtccgt acccctgctg
    20041 gcggcgccac agttcgcgat cgactgtgtc aagtttgacc ggaacgccgg ccggcaaacc
    20101 ggtcagcgtc gcgacaaggc attcgccgtg agactcccct gctgtttcga agcgaaacat
    20161 cgacaatgct ccatcctatc ggatgccgcc tcgaagcggc aacgcctgca gcgtagaatg
    20221 gatttgtcgg ctccttgctc aaactctgca tgcacgcgaa tacccgggtt ctgtcgttgc
    20281 tcttatttgc cgcagtcaca ccgctcttct cggaggtagc gctcctcgtc ggagagccgt
    20341 ttggcaagtt cggtgctctc aacccgaccg gacatgcggc aatctacctg tcgggagtgt
    20401 gcgcggagtc gccgataaag ctccgccttt gccggccggg cgataccggc gtggtgatca
    20461 gccgctataa ccggattgcg gggtacgact ggatcgccgt tcccctggtg ccttacctgt
    20521 acgccgttga gcggcccgag gatgtgcccg cttccgccga tgatctggcg gtcgcccgtt
    20581 tgcgggaagg ctatcggcga tcgcacctct ggacgcttgt gcccagcggc ccggaaaatg
    20641 atcctgccag cggggactgg acccaactgg ctggcgccgc ctacgatcgc accatctatg
    20701 gcttcgcgct cgaaaccacg ccggaggacg acgcccgcct ggttcgctac ctcaattcga
    20761 agcccaaccg gaagcggttc aatcttcttt accggaactg cgcggacttt gcgcgcggca
    20821 tccttaattt ctactatccg cgtgcgctcc gccgcagccg catcgcggac gctggcatta
    20881 ccacgccgaa acactctgcc aaaacgctgg tcaactattg ccggcgccgg cctggcctgc
    20941 gcctttcgca attcgtgatt ccgcaggtcc ccggtggacg ccccagcaag aaactccggg
    21001 gagtaagcga atccctcgtc tggtcgaaga agtacgtggc gcccctgctg ctctttcagc
    21061 cctgggtggc ggcgaccgcg gccgcggcct acctgtcgaa aggccgcttc gatcccgccc
    21121 ggcattgtga gacagtctgc gagcccggca cgctggaatc ctgcatttcc ggctccggga
    21181 ccggcacgat agcggcctca gccgaccgcc cagccaggct gagccgcgac taaaaggggc
    21241 ggcaaaaatg cttcgtctgg gacgcaccgc actatctctg tgataatgaa agtacgtcat
    21301 gagcgatcga ggtccgctgg aggatttggt tcgccgggcg cgccggcgca tcgtccggcg
    21361 cctggtcgcc gcggagcttg ccttcgctgc cgccgtcgcc atggccggcc tcatcgcgct
    21421 tctcgtcctc ggcacccaga tcctcgaatg gtattggctc gtcgtcctct tcggcggcag
    21481 cctcgcgtgg gggctctacc gcacgttgcg ccgcgtcccc tccgcgtatt acgtcgcgcg
    21541 caatgtcgac cgcggcctcg gcctagagga ttccctctcc accgcgtatc acttccttaa
    21601 tccgcccggc agcctcaaag tctcgccgga cgtccgtgac gctcagcggc ggcaggccga
    21661 agagcttgcc ggccgggccg acgtcaaaca ggccttgccg cttggtgttc cgcgcggcct
    21721 tcgcgccgcc ggtctcctcg gactcgttgc cttcggcctc ttcgccctcc gctacgggct
    21781 gacgcattcc ctcgatctcc gtccgccgat cgcgcgagcg tccttcgacg tcttctctgc
    21841 gaaaacggtc gagaccgcgg gcttgccgaa accgcgcccg ccgcgtccgg gcaacgaacc
    21901 gattcccgcg atcggcatga ccgcggacga acgcgccgcc aagcacgact cccgccagtc
    21961 cctcgccacc gaaccgggcg aagccgaagc gtccagcgtc cccggggatg cgctccgtaa
    22021 ggcggaaggc ctccccacgc gcgatgccgc cgatgaacag gcggaggctt cctccggcga
    22081 gcctgcgccc aacgcggatt cttcccctgc ggcggagggc gataacgccg ccccgtcgtc
    22141 gccacgcaag cagaatgcga agtccggcga aaactccgac ctcctgaaca aactgcgcga
    22201 tgcgatggcc gacctgctct cacgtctgaa aatcaagccg ccgtcgagcg gcgagatgca
    22261 gaccgccgag ggcaagggag acacggagtc ccggcagcgg cagggcaagg gccagaaagg
    22321 cccgcccggc gccagccgtg aggacatgcg tgcggacgcc aacgccgaac aggaggccgg
    22381 cgaagagagt gaaggcgccg agtcccgtga caaggccgcc ggcaaacccg gccgccgcag
    22441 cgccgacacc gccgccaatc gcaaggaacg cagcggcgtt ggcaaagagg aaggcgacaa
    22501 ggagatccgc gaagcccagc agctcgaggc gatgggaaaa atcagcgaga tcttcggcaa
    22561 gcgcgccgaa aatctgaaag gtgaggtgac cgtcgaggtc tcctccggcg accagcagtt
    22621 aaaaacgcct tactcacaga gcgccgccgc tcactccgac accggcggcg agatccaccg
    22681 cgacgaggtc ccgctcatct accagcagta cgtgcagcag tactttgagc aggtccgcaa
    22741 actcccaccc cccaagtgag cgcaaccgga gacaggctac tctgccctga gcgaagcgag
    22801 cgggcagagc tgcctgtccc cgtgattctg gaacctcaag cttcagcgag cggccctact
    22861 tccccgtcgt gcgcatccgc tccgcgatgc tcgccgcgta gtagttcacc acctgcgcct
    22921 ctccgcgcac cacttcgtaa tacgccgctt tcagcaactg atctttgcgg ttcatgatcg
    22981 tttcgcggat cgtctgctgc acccgcggat cctgcagctc gcgctggccc gccggctcct
    23041 tcgacatcac tttcagaatg cggtagcctt ccggcgtccg gataatcggc gagatctgcc
    23101 ccggctggag ctgcatcacc atcttccgca gctccacgtt cgccttctcc agcgccgact
    23161 ccggaatgaa cccgaggtca ccgccgttgg ccgccgtgtt cgggtcttcc gaataattct
    23221 gggccagcat ggcgaagtcc tcgccctgtt tcagccgcgc ctccagcatc cggatcttct
    23281 ttcgcgcctg ctcctcgctg ttcgcctcgt cccgtttcag attgcgtgcg tccggattcg
    23341 cccccggcgt caccagcagt tgcaggagat ggacccgcgg ctccgcctgg ttgaacgccg
    23401 ccttgttcga gttgtagaag tccgtcacgt ccttgtcgga gatgttgatg tgcgacgtga
    23461 tctccttgtt aaaaagcttc tggatgctca ggtcgcgccg gagctgagtc tttagctccg
    23521 ccaccgtcat attccgcgcc tccaactgct tctggaactc ctcctgggtg tacggcgcct
    23581 tcagttcgtt gaacttcgca tccacgtcgg agtccaccgc catcagcccc agtttctccg
    23641 cgcgctggag catgatttcg ttgtccacca tgctgcgcaa tacttcgagc ttttggatcg
    23701 ccagcaggtc ctcgctggcg cgctcgtcgc gctgcgggaa ctgtagcttg tactggcgct
    23761 ccaggtcctc gtgcgtgatc acgcggttgt tcactgtggc cgcaacattg gcgggcggcg
    23821 acttcctgca gccggggatg gcgagcgctc cggcggccag aacggcaagg aggggtaagc
    23881 agcgggatga catgattcgc tacgttattg tatcggagcc gccgcctccg ccgcagccgc
    23941 ggcgggagcg tgatctcata tgcaccggac acacggattc atcaccttca ccgcggcctg
    24001 tttcagcttg ctcgccgctc cccgccaaac tcccggcgtt ccgtccgggc gctcgtcctt
    24061 cgaatggcag accgccacag ccgctgagct gggcctcgac ccggcaaagc tcgacgccat
    24121 gaaggacgcg ctcgccgcgc gcggcacctc cggcctcctc atcgttcgcc gcgggaagat
    24181 cgcttacgag tggtacgcgc ccgcgagcgg gccggctaaa ccgcactaca ccgcttccat
    24241 ggccaaggcg ctcgttggtg gaatgtccct gctcctggct ttgaacgacg gccgtctcgg
    24301 cgccgatgac gtcgccgcga aatacatccc ggaatggcgc aacgatccac gaaaagcccg
    24361 catcaccatc cggcagctcg cctctcacac ctccggtatc gaggacgccg aggaagaagg
    24421 aaaacgtcac gatgagctca ccggatggaa aggtgctttc tggaaacggg atcccgatcc
    24481 gttcactatc gcgctgaccc gcgcaccggt cgtctttgac ccgggaaccg cgaacgcata
    24541 cagcaacccc ggcatggccg ctcttgccta tgccgtgacg gccgccctga gaacggcgcc
    24601 gcagaccgac atccggactc tactcaaaca gcgcgtcatg gatccgctcg gcattccgga
    24661 aaaggaatgg tccatcggct atgaccgggc ctacgaggtc gatggcctca gcctctacgc
    24721 caactggggt ggcggtggct acaccgcccg cgccgccgct cgcgtcggcc aactgatgct
    24781 gcaccggggc gaatggaatc accgccgcct gatcggccgc gagtgggtac ggcaggcgct
    24841 cgccccctcc ggcctccccg atcagccgag caccgccgcc gaccccgccc ccgcctcggg
    24901 cctcgcctgg tggatcaacg tcaagagcgg atggcctcgc gtgccccgcg acgccttcgc
    24961 cggtgccggc gccggacatc agttgctgat tgtgattccc agcctcgacc tcgtggtcgt
    25021 tcgccacgga gaggccctcg aaccccccaa ggccggcggc agcttctggg cgccggtcgt
    25081 tcgctacctg ctcgacccgc tgatggacgc ggttgaggtc aagacgccat acccgccgag
    25141 ttcggtgatc cggaaaatcg acttcgcgcc ggaatctacc atcgttcgga aagcgctcgg
    25201 cagcgacaac tggcccgtca catggggtga tgacggagaa cagtacacct cctacggcga
    25261 cggctgggga ttcgagcccc tgctcgaccg gaagctcagc atgggcttcg cgacaatcgt
    25321 cggcggcccg aaggattttg ccggcgtcaa catccgttcc gcaagcggcg agcgcaccgg
    25381 agacggaaaa gaaggcgcca aggcgagtgg aatgttgatg gtcggcggcg tcctctacat
    25441 gtgggtgcgc aacaccggca acgcgcagct tgtctggtcc tctgatcgcg gccgcacgtg
    25501 gaaatggggt ttccggttcg acaccgcctt cggctccccc gccttcctga acttcggacg
    25561 caactacgca ggcgcgcgcg ataactacgt ctacacgtac tctcagaacg gacccagcgc
    25621 ctacgaatcg gacgacgcct tgctgctcgc gcgtgtccgg aggaacaaga ttcgggaacg
    25681 cagcgcctgg gagttcttcg agcggctcga caccgccggc catcccgtct ggacgggcga
    25741 catcgcccgc cgtggcgagg tgtttcgcta tcccggcaga tgccagcgcg tggacatcgt
    25801 ttacaacccc gggctgaaac gatacctgct ggcgctgggc tttgaccact ccgggggatg
    25861 gggcatcttc gacgctcccg agccgtgggg tccctggacc acggcgttcc atacggacga
    25921 ctggggcttg ggcggcacgc acggatatcg ccttcccgcg aagtggatcg gcgccgccgg
    25981 aacggaaatg tatctgatct tctccggcgc caaccgccca ggcatcacca ccgacgcctt
    26041 ctgcctccgc cgcctcaccc tgaccatgaa ataaaaaccc gggacagtac actcatttcc
    26101 ggatttcgag cggcaactgc cgtccgtccg cccctgccgc cgcgctctcg gtccgcggtt
    26161 tcttcggacg cccggccggc agcggccgca attggcgccc cgtctttcgc tccagttcgt
    26221 cgatgaactc ctccgacccc agcggccggc cgcgcagggt cgcttctcgc aggcgctcgg
    26281 ccagcgcttc ctctgcgacg ctgctcgcca gcaccatccg ccagcgctcc ggcgtgtact
    26341 cctgcgccca cggtcccagg tcgagcaggc ccttcgggtc cggcataccc gtgagatgag
    26401 ccgcggcgct cgaccacctg tactgcccag cctcccgcac cagtccggcc cgcaccgggt
    26461 tccgttcgac gtacgcgacc gcacgccaca gatggatgcc gtccagcggg cacgagaaaa
    26521 agcgagcctg ccacacatga ccgcaggtct gccgcacgat gttgaagtgt ctcgcgaagt
    26581 ctctgtgcgc ttgcccgagc gttctcgcca gcgacagcgg ctgctccggc acgcaaacca
    26641 ggtggacgtg gtttggcatc aggcaatagg cccagatccg aagcccaagg cgtctggagt
    26701 acacccggag caagtcaaga taagccggcg ctcgctgtca gtgtgaaaaa tggtttgctg
    26761 gccgtttcca cgttgcgtga cgtgatacgg cagaccctcg accgccgctc gcgcgattcg
    26821 agacatcaca cactcagtgc gtgaggtcgg cgcattctct caccgaaaac cggaaatgag
    26881 tgtactgtcc cggctttatg ttgccatccg ctccagtgtc agcagattca gcatcaactg
    26941 gcggggcggg tggtagttgt cctgccgggt gacgtgcggc tggaaggtca gcttgcgatc
    27001 ggcgaacagc atatagccgg atttgccgtg cgggcgcgcc gagtacttcc ggtcgatcag
    27061 atcttgcgcc atggtgaagt agcgcgcggc ccactcggcg cgcgtgcgtt cgtagatatt
    27121 gagcgcggcg atcagcacct cgttcagtgc ccacagcggc ttcatgtaga agtactcgcc
    27181 cgtgccgcga aactcgagat cagtcccgac cggccggtcc gccggccact ggtagcagcc
    27241 ctgatcgaca ttgatccact gcgccaggcc gccgtacacg taatcccagc ccacgtcgag
    27301 gtgccttcgg atgcgctccg cggcggtttg ccagagcttg ctgtcgccgc ggcggtccgc
    27361 ctcgtccatc accatccata gcgtctcggc gctgtggccg agcaggctct tagtggcctc
    27421 ttccggcgga cggctgaagt cgaagttcag cagctcgttg ttcaggccga tgtccgggtt
    27481 gtagtgccga ttgatgattg cgtccacgca acgctcggcg atctcggcga tttcggggtc
    27541 cttccagcgg cgcagcatct gggtggcgat gcggaggttc accatccaca ttccctgcgg
    27601 gcggacgcct ggcctgcccg ttcccggcag ttgcaccgac ggcgagttga tgtagctcca
    27661 gagcttcttc atcagcccga gagacagctc gtgcgcctgt gcgtcgccgg tagcggcggc
    27721 gaactcctgc aatccttcgg cgccgaagta catgccgtag acgtctcccg cgaacggccg
    27781 cacgagtttg ccctcgggtg tgagcgtctc ggcccaccag ccatccggct gcggcccgtg
    27841 cttcagcatg aagtccttgg ccttgcgcgc gatttcgaga tagcgcgcct ccttgccgaa
    27901 atgctgatag aggaagctgt agacccagac gccgcggccc tgaaaccagt gaaacttgtc
    27961 ggtcttcacg cgcgtaccgt cgtagtcgag gctgtggaag aagcctccgt gttcgtggtc
    28021 gacgccgaac ttttcccaga acggaagata ctcctcgaac agatcgtggc gatacttctt
    28081 gcgagcttcg gcgatgttca tagctgcctg cctggattct tctttcgggg cgcgcgagac
    28141 cccgcacgcg gcgaggggcg cggccgccgc ggatgcaagg aacgagcggc gtctcacgtc
    28201 cacgcatcct atcacgccgg tgccgcaggc catcgttgtc tgtggcctgc ggtcttcgct
    28261 aaattcgcct gcggggccag gcttactccg ccctgagcgc agtttgcgag cgggcagagc
    28321 tgcctgtcct cctttacccg aaagaattct aaaactcgat ccgcaggccg acccgtccgc
    28381 tgcgggggcc cgctccgtag cgagagtaca ggctcgttat cctgccgacg ctccccgaac
    28441 gtatgttggt gttcggcgag ccatagttcg gatggttcag cgcgttggta aacgtcccct
    28501 cgagactcgc gcgaagccgt tccgtgatac gaaactgctt gtgcaatccc gcgtccagga
    28561 gtagcgcgcc aggaccctcg atcacattgg gcgccgagtt tccgaagcgt cccacgttca
    28621 cgtccggccg cccatcgccc gtaacatcac ccggcacgac gaacgccgtt ttgtcgaacc
    28681 atttcgtgat caccctctcc gagctgggga agttgccgtc gccgacccgg tccgggcgct
    28741 gggtcccggt ggagttggtg ttggagacgt cgaatccgct gaaagtcggg ctgaagaaca
    28801 ggccggtctg cgcgacgagg atgctactca gcgtccagcc gccgaaagcc aggtccgcgg
    28861 ccctgggcag gttggacagg aacgccttgc ctcgaccgat cggcaactca aacaataggg
    28921 tccccgcaac tcggtgtcgg ttggtgtagt ccacgtttcc ccattcggcg ttgaggttgt
    28981 atggattcaa gggcgtgcat cctccgtcgc cgtccgtctg gcaattggtc atgttttttg
    29041 cccacgtcca tcccaactgg tagtaaaggc cgtttagcat cttccgttca gccaccagat
    29101 gcatcgaatg gtagttgtgg ttgccgccgt tcacccgcac cgtgacgtct cgcagcgccg
    29161 ggaacggcct cttgcttggg tcgaacctgt cgagcgaagg cgccacctgg tttacgttct
    29221 tcacccacac caactggcgc gtgctcgtcc cgatgtaaga gaggcggact cccatcttga
    29281 acacttcctg ctcgagagtt agattccatt gctggatgta agggttcctc agcttggggt
    29341 cgattgcgct gaagctctga gcgccgatcg cgccaaatcc cggcggaaac gcgttcggga
    29401 actggaacaa cggcgtgccg cccgtgattg cgttggtgaa ggactcacgt gaaatgaacg
    29461 ggccgccggt tccgagacgc cacagcgatg atgtcaagtc gtcggtgtaa atcccgtatc
    29521 cgccccggat caccgtccgg ttgctggcga atgggcggta ggcgaaaccg aaccgcggca
    29581 cgaaattgtt taggtcggtg taccagaggc tgcgcggcac tccggcctcc tcggccgtca
    29641 cgaccggcac gaggttcgac ggaaacagag gattcactcg cgacagggcg gcttcgcttg
    29701 gcacgatcac tctgccggtg gtcacgtcaa aattgaagaa attatcgtcc ttctcgtggg
    29761 tgggcgggtt gtactcatac cgcatgccga ggttcagggt cagtttcggc gtgatcttcc
    29821 agtcatcctg gacgaacagg cccatgtccg tgctcagcgc atggatcagc ggcgcggcgt
    29881 tcgcgcggcc ggcggtttgc ggaatgccca ggaggaagtc ggaatagtca aacccgctat
    29941 aggagccggc aaaagtgaac tggccgaact ggaggaccgg gaagctggtg ccgcctggga
    30001 aattcgtccc ccggttccag cggatgtcaa taccggactt gacgacgtga ttgccccggg
    30061 tccatgtcac gttgttaacc acctggtgga tgcgctcgct cggatcctgg tagatgtcgg
    30121 tcgcgccgat ctcctcgaag ttattgaagc tgaagacggg cgctcccttg tccagttccg
    30181 ggttccaact gatgccttgc agaccgaact cctggattag cgccggcccg tcgagcgggt
    30241 tgaaggccgg gttcttcgtc cgcatgattc cgaagcggaa ctcattgatc agcgttggcc
    30301 caaacaggtg gttgtcggag atcgtaatcg ttgtgctgcg ccggtcttgg tcgcgcttcg
    30361 ggatcgtcgg gagattgtag tcccaaagat tggccccagt gcggttccag gagaagcgaa
    30421 cgaacagaga attcgccgcc gagagctggt ggtccagccg cgcttcaacc tgggtcttgt
    30481 actgggaact ggcccccgtg ccccgccagt tctgctggta gctgttcgct gagccgaaat
    30541 tgggcaccgg gaagaatcgt tcctgaatct tgagagaact ctggctgagg cgctcctccg
    30601 ggatcatatt gccgttgaac ggcgttcctg tcagcggatc gcgtatgacc gtgccgggaa
    30661 gcagagacga gaagtcgccg ttccggaacg cggccgtcgg cacactggag ttgaaaattc
    30721 gctcgttgcg cagcggaaac cgctcgtagg tcgtaaagaa gaaagtccgg tttcggacga
    30781 ccggtcctcc gatgctgccg ccgtaattgt tttgaatctg aaacggcacg ctgctggaga
    30841 atgtgttgcg cgccgccagc cgggaattcg agtggtacca gaacaggctg ccgtgatact
    30901 cgttggtgcc tgagcgcgac gtggcgtaca cgccgccggg cagactcgcc tcggccttgt
    30961 cgttggcgag ttgaacgcgg atctcgcccg tcatctccat gttgctttgc gccggcccga
    31021 tgctgttgcc gaacagcggc gaccgttgcg atgtcccgtc cacggtggcg ccgaactggc
    31081 ttccgcgggt tcctgccagg ctgaagttcg agccctgccc gcgcgtcgcg cctgggatga
    31141 aagtcagcat ctcgtaatag aagccgtcga actgcccgcg cacattcata ggcatcttct
    31201 caagcacctg attgcttgtg accgccgcga cctcagctcc ttcggtgttg atcacaggcg
    31261 ccgccgcttc cacgcgcacc gaagtcgcca cttccccgac ttccaggcgg acgtccgaac
    31321 ggaccgtcat cgatgtctgc aggagaatgt ttgcgacgac caccttcttg aaccccgctt
    31381 tttccgcggt tacctcgtag agccccggga tcaactgggt cacctcgtag ttgcccgtgg
    31441 acgccgtggt agtctggcgt tcgatatttg tctcgacgtt gcgcacgaca acgttgacat
    31501 ccgggaccaa agcgcctgtt gagtccgtta ccgaaccgcg gatggtgccc aacgtggatt
    31561 gagccatcac ctggatggcc cagaaaacaa aaaggctggc gaaaatcgca agcctgacaa
    31621 tcccctctcg ccttgcgaac attgcatcct ccattcgccc ttggtgttgg ctgcgaaact
    31681 atccccgtcc gctggcgccc ctacgccagc aacacaagta cctgtggcga tcgtataccg
    31741 ggattgtctc tatgtcaatc gggattccct tgattgagcc ataggccaag ccatcgcctt
    31801 ccgtaccaat tgtgtagggc ggttcccgga agccgcccga cagttgaaac ctggatcgaa
    31861 acagagcgcg ccgggcggcc ggcttgaccc agcagcaagt ggcggaccgc gcatacaagc
    31921 gcgttgctgc gcgtgtcacc aaggcacgag caagcagttc cagtcagggc tggacatctg
    31981 ccgaatttat ggggctggtg catacggcta caaacaaacg ccgcatattc tttggcaagt
    32041 ccttttcgat acgggtgccg tactgatagt agtactcaca tcggaagaat tcctcaagca
    32101 gtttcttgat gcttgcatcg gtccagacct gtttatgata ggggttcgtt gcccatcctt
    32161 tgcgttcatc cagaggagtg gacaagagga gcatctttgc cgtcttttgt acgttgccaa
    32221 ggaagaattc aggattcgct agatgctcga tcgtttcgaa gctgacaata acgtcataat
    32281 gctcggacca cgcttgcttt tccaaatcga taatgaattt ctggccgagc ggttctcggt
    32341 cgtatccttc cactgacttt gccaccgtgc gcaagatccc catgccgtag ccggtcccac
    32401 tcgcgatgtc cgccactgtc ttccctgcac agtactgttt cgcaaattca taacgaacct
    32461 tgtgctctct gtcgataatt gtcagctctc tcttccctct tatctcgatc cgctcttcag
    32521 aatggtaccg ttcccacatc gcgcggctga acttcgtcaa cgtaacgatc gcgatggccc
    32581 acgtcgctat cagcaagatc cacttggtcc aaacccgcag ccgtgaagtc acaagcaaaa
    32641 ttgtacctct tcgatttttc actacccaga gcgggcagag ctgccggcgg ggacaggcct
    32701 actctgccct gagcttgcga gcgggcagag ctgccgacgg ggacaggctt actctgccct
    32761 gagcttgcga gcgggcagag ctgcctgtcc cccctacccg
//
LOCUS       DATIFQ010000406         2461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1667269, whole genome shotgun sequence.
ACCESSION   DATIFQ010000406 DATIFQ010000000
VERSION     DATIFQ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667269"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VLE22_20180"
     CDS             <1..440
                     /locus_tag="VLE22_20180"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB16781.1"
                     /translation="PRIVVAVGLPGSGKTTYLERIGAPALSTDAIRGLLADDPTDQSL
                     HGRVFATLRYLLRHRLEIGRPLTYIDATNLTRRERRAWIRMGELYGCDVEALYFDVPV
                     EVCLERNRRRGRVVPEDAVRKMAAKMAPPSLDEGFSRVTVVRA"
     gene            complement(502..1833)
                     /locus_tag="VLE22_20185"
     CDS             complement(502..1833)
                     /locus_tag="VLE22_20185"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HSB16782.1"
                     /translation="MPIIRAQVAGIDLGSQRHWVCAPAKDGLGREVADFGATTPELMR
                     LVHWLRERGVESVAMESTGVYWVAPHEVLEAAGVEVLLVDTRQLAGVPGRNKKTDPTD
                     CEWIQRLHSCGLLAGSFRPPEAVCMLRTLVRDKGTLVAEGADWVRRMQKNLDQMNVRV
                     HRAVSDLDGMTGMRIIRAIVAGEREPHKLARLRDPRCRKTEPEIAEQLTGHWREDHLF
                     SLRQGLKMYDAIQKRIADYDHEILRCLAQMAPEERRAASAPPMQNRQKAKSFKRRGQE
                     TMREALYRMSGVDVTQIDAIGVETVEVVLSEYGPDLSRFATEKQFVAHASLAPHVAKS
                     GGKPVKKKKRNSASTRVAAALRMAALSLRHSETALGAYYRSVARRIGGDVAVFATARK
                     LAILIYRLLRWGKAYVDEGAEAYERRFQQRRTTSLAARAKELGYQLVPVKA"
     gene            complement(2138..2344)
                     /locus_tag="VLE22_20190"
     CDS             complement(2138..2344)
                     /locus_tag="VLE22_20190"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16783.1"
                     /translation="MAQSRQAGEVKLRLFKGNVQPVSRTSPYSLYSLDIASFTMGASY
                     DQRDAQGFINLVGLPIRVRAAVTK"
BASE COUNT          402 a          844 c          727 g          488 t
ORIGIN      
        1 tgccgcgtat cgtcgtggcc gtcgggctgc ccgggtcggg aaagacgacg tacctggaac
       61 gcatcggcgc gccggcgctc tcgaccgatg cgattcgcgg attgctggcc gacgatccaa
      121 ccgaccagag cctgcacggc cgcgtgttcg cgacactgcg ctacctgctg cggcaccggc
      181 tcgagatcgg tcgcccgctg acctacattg acgcgaccaa cctgacccgc cgcgagcggc
      241 gcgcgtggat ccggatgggg gagctgtatg gctgcgatgt ggaggcgctg tacttcgacg
      301 tccccgtgga ggtgtgtttg gagcgcaacc gccgccgagg gcgcgtggtg cccgaggatg
      361 ccgtgcggaa gatggcggct aaaatggcgc cgccatcgct ggacgagggc ttctcgcggg
      421 ttacggtagt ccgggcgtga tcgaggcagg actgaagtcc cgcgcaggct ggcctgtgac
      481 ttcctggctc aacgcatatt atcaggcctt tactgggacg agttgataac cgagttcttt
      541 cgcccgggca gccaagctag tcgtgcgccg ctgctggaat cgcctttcgt aagcctcggc
      601 accctcgtcg acgtacgcct tcccccaccg aagcagccga taaatcagga tggccagttt
      661 gcgtgccgtg gcgaataccg cgacatctcc gccgatccgc cgcgccacgc tgcggtaata
      721 agctcctaag gcggtttcgc tgtggcggag tgacaaggcg gccatgcgta gggcggcggc
      781 gactcgggtg ctcgcactgt tgcgcttctt cttcttcacc ggcttgcctc cgcttttggc
      841 tacatggggc gccaggctgg cgtgagcgac aaactgtttt tcggtggcaa agcgacttag
      901 gtctggtccg tattcactca gcacgacttc gaccgtctcc acgccaatcg cgtcgatctg
      961 ggtcacatcc acgccactca tgcggtatag cgcttcgcgc atcgtttctt gtccccgcct
     1021 tttgaagctt ttggctttct ggcgattctg catcgggggc gccgatgctg cccgccgctc
     1081 ctcgggtgcc atctgggcca ggcagcgtag aatctcgtgg tcgtaatccg cgatccgctt
     1141 ctggatcgcg tcgtacatct ttagaccctg ccgcaagctg aacaagtggt cctcgcgcca
     1201 gtgcccggtc aattgctcgg cgatctccgg ttcggtcttg cggcagcgtg gatcccgcag
     1261 tcgtgccaac ttgtgcggct cccgctcgcc cgcgacgatg gcgcgtatga tccgcatccc
     1321 cgtcattccg tccagatccg aaactgcccg gtgcacgcgt acattcatct ggtccaggtt
     1381 cttctgcatg cgtcgcaccc agtccgcccc ctcggccact agagtgcctt tgtcccggac
     1441 caaggtccgc agcatgcaaa ccgcttccgg cggccggaac gagccagcca gcaatccaca
     1501 actgtgcagt cgttgaatcc actcacagtc ggtcgggtcc gtcttcttgt tgcgccctgg
     1561 cacgcctgcc aactggcgcg tgtcgaccag cagcacctcc acacccgccg cctccagcac
     1621 ctcatgcggg gccacccaat acaccccggt gctctccatc gccaccgatt ccactccccg
     1681 ctccctcaac cagtgcacta ggcgcatcaa ctcgggcgtg gtagctccaa aatcggccac
     1741 ttcccgtccg aggccatcct tggccggagc gcacacccaa tgccgttgac tgcccagatc
     1801 gatccccgcc acctgcgcgc gaatgatcgg caatccttcg attcgcctct ctcgatcggg
     1861 ttccgatcgg cgattccttg ggtcctccac ttgtccacct ctctgaaccc gaccgcccgg
     1921 gttggccgaa catcgattct ggcctagggg agtgcgacgt gcgcctcacc aattcttaat
     1981 cgccatcccg gagccacgct gaagaagggg cattacgcac caatgcaata agggccataa
     2041 ctgcggtcgc gttctctagc agcttagccg cagcgcagcg cgtacagtca caggcacctc
     2101 aatccggctt ggccggtggc ccaggctgaa gcctgcccta ctttgtcacg gcggcgcgga
     2161 cgcggatggg caggccgacc aggttgatga aaccctgcgc gtcgcgctga tcgtagctcg
     2221 cgcccatcgt gaagctcgcg atgtcgagcg aatagagcga gtatggcgac gtccggctca
     2281 cgggctgcac gttgcccttg aacagccgca gcttcacttc gccggcctgc ctcgactgcg
     2341 ccatccggaa ctcggtgcgt gccgcctttc agggcgagca gactcgccgg gtccgccata
     2401 aactgcagtt agttcgcggc gatcacaccg tcgaggccca catgctcgta accaccgcgc
     2461 c
//
LOCUS       DATIFQ010000407         2216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2242903, whole genome shotgun sequence.
ACCESSION   DATIFQ010000407 DATIFQ010000000
VERSION     DATIFQ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242903"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(32..224)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(360..656)
                     /locus_tag="VLE22_20195"
     CDS             complement(360..656)
                     /locus_tag="VLE22_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16784.1"
                     /translation="MPTTTYSSTATMVRAQKFTFDGRFLLAFAYGHPPDGSVYFHSLT
                     GDKWGNVFVTVRTKAGYEGTLESNVGKKVSMAKYNNNGDFITTLRLSTREHSES"
     gene            complement(1075..>2216)
                     /locus_tag="VLE22_20200"
     CDS             complement(1075..>2216)
                     /locus_tag="VLE22_20200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB16785.1"
                     /translation="VPASIRERVRSAYLRRFYYRMYPENAIHRASGPEGRPATESQSI
                     ETPSCPSTYQALVKQIDQQSDTAFEGVPDEQVAEVLSWLLVPLARNGFSSPARKSALF
                     RKAERAGIHLLPVHYYSPVPDTSRLDERDWEHRFDRNGGWNLNAEGQVALLSQLAPFA
                     GELEEIRRSPSSACAFSWDNGTFCETDAAVYYSLIRHLRPRQVLEVGAGNSTLIAAEA
                     CVKNGHTELQAIDPYPLEILRQKVPGLNRLIEGPVQRVPVARFGELCENDILFIDGSH
                     VCRFGSDLNYLVFRVLPALQPGVIIHFHDIFLPWEFPRDWIVDRQLFWNEQYLLLAFL
                     LFNDSFEVLLGTQYLGREMPGELLRVFPFLRVPGGASFWIRRKTR"
BASE COUNT          466 a          699 c          617 g          434 t
ORIGIN      
        1 agccgcaaag gaaggaaacg gcgtttctac acttcccgat agcgctccat ccgctttcga
       61 cggatgacag tgggcaggtc ttaaccttac cccccagtcg ccccggagtg tgcttcccga
      121 tgcgacttcg gcggattcaa cctccagccc ctttcgccgg acctccggag caccccggaa
      181 cggcctgccc ggctactgat ggcttccgcg cctctaccaa gagtaaagtt caggatagcg
      241 aagcagagaa tctcaagtca aattcactcc gcacactact cctcggcgaa aatctcgacg
      301 ccagactcgg tcctgccgtt gaacagagcg taaaccgtgc cgtcgccatc cacgaccgac
      361 tagctttcgc tgtgttctct ggtcgaaagg cgaagtgtcg tgatgaagtc gccgttgttg
      421 ttgtacttcg ccatgctgac cttcttcccc acgttgcttt cgagcgtccc ctcgtagccc
      481 gccttcgtgc ggaccgtcac gaaaacgttc ccccatttgt cgccggtgag gctgtggaag
      541 tacaccgatc cgtctggggg gtggccgtag gcgaacgcga gcaggaagcg gccatcgaag
      601 gtgaacttct gcgcccggac catagtagcc gttgacgaat acgtcgtcgt tggcatccat
      661 gaacaggccg tggggcgtat tgaactcgcc ggcgttgatc ccgtctctcc cccagtggaa
      721 gaggaacgcg ccatccggcg cgaacgcgct cacgcgcata ttgtcgacgt ccgtgacaaa
      781 cagccagccc gttcgatcga aggcaagccc gtgcccgcgc aggatctgcc ccggtccggt
      841 tcctttcccg gcgaagattt tcaggaactt gtcgttgcgg tcgaacacct ggatacgcgg
      901 ccggtctccc ttcacgtcgg ccacctagat cttgccgctg gccagcgtct cctccgctcg
      961 cttgatcgac cgccagcgcg cggcgacgag agttgcacga cctcggcttc agacctcatt
     1021 cggccgctat ctgctcccga tgagatgtgc aagaggcaca ggattagcct cggcttacct
     1081 cgttttacgc cggatccaga aactcgcgcc tcccggaacc cgcaagaacg gaaacacccg
     1141 cagcagttct ccaggcatct ctcgccccag gtattgagtg ccaagcagga cctcgaagga
     1201 atcgttaaac agcaggaaag cgagcagcaa gtactgctcg ttccaaaaca actgtctgtc
     1261 cacgatccaa tcgcgcggaa actcccacgg cagaaatatg tcgtggaaat ggatgatcac
     1321 tcccggctgg agcgctggca agacacggaa taccaagtag ttcagatcgg agccaaaccg
     1381 gcaaacgtga gagccgtcaa tgaacaaaat gtcgttctca cataattctc cgaaccgcgc
     1441 cacgggcacc cgctggaccg gcccttcaat caggcgattc agacccggca ctttttgcct
     1501 gaggatttcg agaggatagg ggtcgatagc ctgcagttcc gtgtggccat tcttcacgca
     1561 agcctcggct gctatcagcg ttgaattacc ggcgccaact tccagcactt gacggggtcg
     1621 gaggtgcctg atgagcgagt agtagacagc agcgtcggtc tcgcaaaagg tgccattgtc
     1681 ccaggagaac gcacatgcgc tgctgggcga tcgtcgaatc tcctccaact ctcccgcgaa
     1741 cggcgcgagt tgagacagga gtgcgacttg accctccgcg ttgagattcc accccccgtt
     1801 gcgatcgaaa cgatgttccc agtcgcgctc gtccaggcgg gaagtgtccg gaaccgggct
     1861 gtaataatgc acaggcagga ggtgaatccc cgccctctcg gccttacgga aaagggcgct
     1921 cttgcgcgcc ggcgaggaga agccgttcct cgccagagga accaggagcc agctcaacac
     1981 ctcggccacc tgttcatccg gaacaccctc aaaagcggtg tcagactgct ggtcgatctg
     2041 ctttaccaac gcctggtaag tcgaaggaca ggaaggggtt tcgatcgatt gagactcggt
     2101 tgctggcctg ccttctggac ctgaagctcg gtgtattgcg ttctccgggt acatccggta
     2161 atagaaacgc cgcaggtaag cgctccgaac ccgttccctg atcgacgcag gaaccc
//
LOCUS       DATIFQ010000408         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_329998, whole genome shotgun sequence.
ACCESSION   DATIFQ010000408 DATIFQ010000000
VERSION     DATIFQ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329998"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            974..1891
                     /locus_tag="VLE22_20205"
     CDS             974..1891
                     /locus_tag="VLE22_20205"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HSB16786.1"
                     /translation="MLHWADENLLELKTGEIGEHYGRYRLHVPEAERAMAKSLERYGQ
                     LSPVVVCRRNERYELIDGFKRLGAARRLSRLDRLSARLLEADERTLKAAIYGLNRAGG
                     RTRELEEAWIIHALVREDGMSQVEVAELLGRHKSWVCRRLALIERLGGKAREDLRVGL
                     LSPTAARQIVRLPQGNQAEVLDAARREALSGAELAGVVDLWLGCSDRGQQNYILAHPR
                     EALSQAKGTIPSGRNPRLSEAGNHVWKRVGLLLDVLGRMEVWLAHNGRAGLTGEDRVI
                     LAPRFEKLSRDAGSVASLSRDLVLEMTAT"
     gene            1888..>2275
                     /locus_tag="VLE22_20210"
     CDS             1888..>2275
                     /locus_tag="VLE22_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HSB16787.1"
                     /translation="MNESTLNEIVRLSHTGASDRTIARMLELDRKTVSRALQQHRRQR
                     TGDTPNQPVRRPSLLDPYVDQIAQLLERYPNLTAVRLHEELQRLGFQGRYGIVKEHLN
                     AVRPRAPKTPVQRFETGPGVQGQMDYS"
BASE COUNT          439 a          694 c          777 g          365 t
ORIGIN      
        1 gaacagtgac gggcatcgcg attgtgtact tgctcgctgg tgaacccatg ccagatgcgc
       61 ccgccgcgcg gagactgggg caaggcgtgc gctcctgtac gcctcagcat cgattgccaa
      121 ggtgtcggtg gcgccgggcg atggggccag aatggcgccg tcaggaaggg gccagggcgc
      181 cgctcggtag tggcagcgag cataccgcca ccggggccgg tccgaaccac gccgccgcaa
      241 ggcggagaga aagcccggtc cccgaagcgt tcctgtagct tacgatgatg gttcgagctg
      301 aaccggaggt ctaccagagc ccgggagagg atcgcagtcc tctccggggc aggagcttct
      361 gggagccaac actcccatgg gccctcccga gaatcctaca gccccgtcgc ggaatcaagg
      421 tcggactgga cggcggctcc gattggtccg ccgtccgcga acccggcgat gcctgctcaa
      481 aggatgtgag cgccgattcc ggccgaagca ggcgcgcgag cgctattgca gtccggagtg
      541 ccggcgagcg gcgcgggact ggtcgtgctg gaaggcgcgg ctgacctacc gggcaacggc
      601 ctccgggaaa gccaaccggc aagcgcaaag ccgccgctat cgcgagcgcg tcaagaaccg
      661 ccaacaaaca gcggcggcgc agacaccgga gccggcgagg gtcatcactc caaacttttt
      721 tcgaggacac ctgcgaccgc cccggatgct atcggggatt cacgtttcag cgccggtcgc
      781 cgcatcaaag attctgttgc cgcgcctgcc ggcgggccat ggaacgagct tggcagcgcg
      841 aacgcctctg gcgccaggct ccgccactcc ggctacggat ctgagccgcc tggctcgtca
      901 gataatcccg acatattgaa gcgaaccggg cgcccggctt accgttgagc tgcgagtgaa
      961 aggaagccat ccggtgctgc attgggctga cgagaacctg ctcgaactga agaccggcga
     1021 gatcggcgaa cactatggac gctatcgtct gcacgtgccc gaggccgaac gggcgatggc
     1081 gaaatcgctg gaacgctacg gccagttgtc gccggtggtt gtgtgccgcc gcaacgagcg
     1141 ttacgaactg atcgatggat tcaaacgatt gggcgcggcg cgacgtttga gccgtctcga
     1201 ccgtctttcg gcccggctgt tggaagccga cgagcggaca ttgaaagcgg ccatctatgg
     1261 gctgaaccgc gcaggcggcc gcacgaggga actcgaggaa gcctggatca tccatgcgct
     1321 ggtccgcgag gatggcatga gccaggtgga agtggcggag ttgctcggcc gacacaagag
     1381 ctgggtctgc cgccgactgg ctctgatcga acggctcgga gggaaggcgc gtgaggattt
     1441 gcgcgtgggc ctgctgtcgc cgactgcggc gaggcagatc gtgcggttgc cgcagggcaa
     1501 ccaggccgaa gtgctcgacg ctgcgcggcg cgaagcgtta tcgggcgctg agttggcggg
     1561 cgtggtcgat ctctggctgg gctgctctga tcgcgggcag cagaattaca tcctggccca
     1621 tccacgcgaa gccttgtccc aggccaaagg gacgattcca tcggggcgca atccacgatt
     1681 gagcgaggcc ggcaatcacg tctggaaacg ggtcgggctc ctgctcgacg ttctcgggcg
     1741 catggaagta tggttagcgc acaatggccg cgcaggcctt accggggagg atcgagtcat
     1801 cctggcgccg cgtttcgaaa agctgtcgcg cgatgccggt tcggtggcgt cgttgagccg
     1861 cgacctggtc ctggagatga cggccacatg aacgaatcga ctctgaacga aatcgtccgt
     1921 ttgagtcata ccggcgcttc cgaccgcacc atcgcacgaa tgttggaact cgatcgcaag
     1981 accgtgagcc gcgcacttca gcagcatcgc cggcaacgga ctggcgatac cccaaaccaa
     2041 ccggtccgcc gtccgagcct gctcgacccg tacgtggacc agatcgcgca actgctcgag
     2101 cggtatccga atctgacggc ggtgcggttg cacgaggaac tccagagact cggctttcaa
     2161 ggccggtacg ggatcgtgaa agagcatctg aatgccgtgc gcccgcgggc tccgaaaacg
     2221 cccgtgcaga gattcgaaac cggacccgga gtgcaaggcc agatggatta ttcga
//
LOCUS       DATIFQ010000409         3126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_714468, whole genome shotgun sequence.
ACCESSION   DATIFQ010000409 DATIFQ010000000
VERSION     DATIFQ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714468"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /locus_tag="VLE22_20215"
     CDS             <1..98
                     /locus_tag="VLE22_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011213396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB16788.1"
                     /translation="GFHTKMEVLQRQAYGFRNFNNYRLRVKVMCS"
     gene            complement(200..277)
                     /locus_tag="VLE22_20220"
     tRNA            complement(200..277)
                     /locus_tag="VLE22_20220"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(240..242),aa:Pro,seq:cgg)
     gene            complement(484..1653)
                     /gene="secF"
                     /locus_tag="VLE22_20225"
     CDS             complement(484..1653)
                     /gene="secF"
                     /locus_tag="VLE22_20225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HSB16789.1"
                     /translation="MELFKQTKFDFLRWKWPFIGASILIMAAGMISLVVKGGPLYGID
                     FRGGAIMDVMFAGQPPIRQVRTALAAKIRGDISVTTLDSREVLIGIDMRGRDERSLQE
                     ARGVMTDTLMGAFSPDKSKLEFNNAAEEALVSRLRDPLQQGNAGLSDEQLRKLVSAMR
                     TFRDTQRSGLLKNFDELAAVPGVTPAVIAVLKQQCTLAPFSIRRVEVVGPKIGKDLQL
                     QALQATMYALAGMLVYVAFRFEWIYGVAAVLAVLHDTIITVGLFSIFNKEIDLTVVAA
                     ILTLVGYSMNDTIVVFDRVRENLKTSRRESLYSVVSLSINQTLGRTVLTSGLTFLTAF
                     SLFLFGGSVLHGFSFALVVGILVGTYSSIFIASPILVAWQNFASARKRPARAVAQ"
     gene            complement(1783..2031)
                     /locus_tag="VLE22_20230"
     CDS             complement(1783..2031)
                     /locus_tag="VLE22_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16790.1"
                     /translation="MSFVSPRIDPKSAVGRAPEQLALEEREALAGKWIALEMYTPKTL
                     PLRRIEAIGDSTRDCIGQLASRGLDPRQFEYTLLNRSY"
     gene            complement(2078..>3126)
                     /locus_tag="VLE22_20235"
     CDS             complement(2078..>3126)
                     /locus_tag="VLE22_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16791.1"
                     /translation="IDFPKDSPVAVVSADWDGGESRVSARGSAMVLDLRTSLILRNSG
                     SQRIRGVSLLVLAQEVSPGGRASVTVPSLDVGPGDTFPVRIDLRLLRPLLVGGGPMVL
                     VSLDGVLYDDLSFFGPNRLNSRRTMTVWEMEARRDRQHLKWVLTAQGADALQKTVLRS
                     LARLDERPRLDVRVSRGRSTNVEAERDVQFAFLAIPDSPVEPLSGLARIAGNEARAPS
                     LEVRNRSNRPVRYFEIGWIIRDREGREFLAGSVPASDPELRLGPGEKSRVTQQASLRF
                     SGAPGRPLSIAGLTGFVSQVEFGDGSVWIPSRAALEDERLARVAGPSPEEQRLIELYR
                     RKGLAALVAELKKF"
BASE COUNT          630 a         1077 c          873 g          546 t
ORIGIN      
        1 aaggcttcca tacgaaaatg gaggtcctcc aacggcaggc gtacgggttt cgaaacttca
       61 acaactaccg gttgagagtt aaggtaatgt gttcttgaga acgtcagcga ctgggcctgc
      121 ccccattaat ggcgtagagc ccggacccat tcttggaccc tgatttggtt ggttgatgtc
      181 tgtaagtctt tgaggaattt ggtcggggcg agtggattcg aaccaccgac ctcctggtcc
      241 cgaaccaggc gctctagcca ggctgagcca cgccccgacg gatcaatgag ttagcggctt
      301 aggtatcatc gcgcaaggct gacggttcac atgccgacgc tcccaaccat gggcagtata
      361 gcactattca accagttcga tcgaatgcct catactgggc agaggcagcg caaatgagca
      421 acatcgcgct actctttcct agccccagcc cagcctcggg cctttccgcc ctccacgcgc
      481 aggtcactgc gcaactgcgc gagcgggccg cttccgggcg ctcgcgaagt tctgccatgc
      541 gacgagaatc gggctggcga tgaaaatcga cgagtatgtc cccaccagaa tacccaccac
      601 cagcgcgaac gagaagccgt gcaagaccga acccccgaac agaaatagcg aaaaggcggt
      661 cagaaatgtc aggcctgagg tgagaaccgt tctgccaagc gtctggttga tgcttaggct
      721 gaccacagaa tacaaggatt cccgccgtga cgtcttcaga ttctcgcgga cgcggtcaaa
      781 caccacgatg gtgtcgttca tcgaataacc gaccagagtg aggatggcgg caactacggt
      841 caggtcgatc tccttgttga aaatcgagaa cagtccaacc gtgatgatcg tgtcatggag
      901 caccgccaga accgcggcca caccgtagat ccactcaaac cggaaagcga cgtagacaag
      961 catgcccgcc agggcgtaca ttgtcgcctg tagcgcctgt agctgcagat ccttgccaat
     1021 cttgggcccc acgacttcta cccggcggat cgagaacggc gccagcgtac attgctgctt
     1081 caggactgcg atcaccgccg gcgtgacgcc gggcacggca gcgagttcgt cgaaattctt
     1141 caacaatccc gacctctgtg tgtcccggaa ggtccgcatg gccgacacca gtttgcggag
     1201 ttgttcatcg gaaagaccgg cgttgccctg ctggagggga tctctgagcc gtgaaaccag
     1261 cgcctcttcg gcggcgttgt taaactccag tttcgacttg tccggcgaaa aggcccccat
     1321 cagcgtgtcg gtcatcactc cccgggcttc ctgaaggctc cgctcgtccc tcccgcgcat
     1381 gtcgatccca atcagtactt cacggctgtc tagtgtcgtc acgctgatat ctccgcggat
     1441 cttggcagcc aacgctgtgc gcacctggcg aattggaggc tggcccgcaa acatcacgtc
     1501 catgatcgcc ccgcctcgga aatcaatccc gtagagaggg ccgcccttca cgacgagact
     1561 tatcattcca gcggccatga tcaaaatcga agcgccgatg aacggccact tccagcgcaa
     1621 gaagtcaaat ttcgtttgct tgaacagttc catgcgatct acttggcccc gcgttcgatt
     1681 ccccttagat gcctgccagt gggcgaaagt ttccggtcca gcttatcctg agactgctgc
     1741 ggaacgccgc tcggcggaac gaggcaaatg cgtcgcgcac agctaatacg accggttcag
     1801 cagcgtgtac tcgaactgcc tcgggtccag gccgcgtgac gcaagctgcc cgatgcagtc
     1861 ccgcgtcgag tcgccgatcg cttcgatccg ccgcagcggc aacgtcttcg gcgtatacat
     1921 ctcgagcgcg atccacttgc ctgcgagcgc ctcgcgctcc tctagcgcca gttgctccgg
     1981 cgcacggccc acggccgatt tcggatcaat ccgagggctt acgaaagaca tgaaacagcg
     2041 attgtaccgc agcgggggcc ttgccctgca accgggccta gaacttcttt agttccgcca
     2101 ccagcgccgc cagacccttg cgccggtaga gctcgatcag ccgctgctcc tcgggcgaag
     2161 ggcccgccac tcgtgccagc cgctcatcct ccagcgccgc gcggctcggg atccacaccg
     2221 acccgtcacc gaactccacc tggctcacga aacccgtcag ccccgcgatc gagagcggcc
     2281 tccccggcgc gcccgagaac cggagcgacg cctgttgggt cacgcggctc ttctctcccg
     2341 ggccgagccg gagctcggga tcggaggcag gcaccgagcc cgccagaaac tcccggccct
     2401 cacggtcccg gatgatccag ccgatctcga agtaccgcac cggccggttc gaacggttcc
     2461 gcacctccag gctcggcgcg cgcgcctcgt tgcccgcgat cctcgccagc ccggacaaag
     2521 gctccacggg ggaatccgga atcgcgagga acgcgaactg cacgtcccgc tccgcctcca
     2581 cgttggtcga gcgtccccgt gacacccgca cgtccagtcg cgggcgctcg tccagccgcg
     2641 cgagactccg caggaccgtc ttctgcagcg cgtccgcgcc ctgtgccgtc aacacccact
     2701 tcaaatgttg ccggtcgcgc cgcgcttcca tctcccaaac ggtcatcgtc cgccgcgagt
     2761 tcagccggtt cgggccgaag aacgacaggt cgtcgtacag caccccatcc aggctcacca
     2821 gcaccatcgg accaccaccg accagcagcg ggcgcagcag ccgcagatca atccgcaccg
     2881 gaaacgtgtc gccagggcca acgtccagac tcggcaccgt caccgaggcc cggccgcccg
     2941 gcgacacctc ctgcgccagc acaagcaacg agacgccgcg gatccgctgc gatcccgaat
     3001 tccgcagtat cagcgacgtt cgcaggtcga gcaccatcgc gctcccgcgc gccgaaaccc
     3061 gcgattcccc tccatcccag tccgccgata caacggccac cggggaatcc ttcgggaagt
     3121 cgatct
//
LOCUS       DATIFQ010000410         4530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1479193, whole genome shotgun sequence.
ACCESSION   DATIFQ010000410 DATIFQ010000000
VERSION     DATIFQ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479193"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VLE22_20240"
     CDS             <1..719
                     /locus_tag="VLE22_20240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB16792.1"
                     /translation="RFRVIYDRPATFRQAFAQFFRQSFEVREFDALKGISFEVRSGET
                     VGIVGRNGSGKSTILKIIARVYRPTAGSVEVFGRVSPLIELGAGFHPELTGRENIVLA
                     GALMGIDPSDMRNRLEAVVSFCELQDFIDAPIKQYSSGMYARLGFAIATEIDPDILLV
                     DEILAVGDEAFQQKCLERMNKFRREGKTIVYVSHDLNSVKTLCSRALLLDSGEIVETG
                     DPEKVIDRYHELLQTSAVQS"
     gene            complement(745..4419)
                     /locus_tag="VLE22_20245"
     CDS             complement(745..4419)
                     /locus_tag="VLE22_20245"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSB16793.1"
                     /translation="MRSATSRRLVTLLFLGSLLVSSWLFERSAQAGGPLIVGAGSAGF
                     GADGVPYTWDLSGATSALVSGGQIQYRTDGGNLGLRDNATANNSVMAMFDKWENVSSS
                     NVAFNRLGPLAPAGAFAGGDVDTVSEFNAVEGSCNTVQQNPIVYDANGGLFQSLGFSA
                     GVIGFAGACAITVSSTSPSRIVSAHAALNGKFIDGINDPAMGNLELTPSQFNGAFIHE
                     FGHFFGLDHSQINLNCDFTPASCPNGSDDLLGVPTMYSFLLDQEESPGVSYAETLSTD
                     DLAWVSKLYPGAGFVSSFGTITGTILFSDGSTPAQGVNVIARQADNPATPENESRRIA
                     VSVVSGFSFTGNPGQTVTADYLPCSPPGSTGCPASGFAGNNSTGSRFGSRNPALRGFY
                     EIPGLPPGDYTIEVESLNPGAFGGSSIGPLSPPIPNPGPDEFFNVGDSATDLPGDVTQ
                     VAVGAGGTFANANIVLNGTPPALDALESNESPGGAAAIFNGTTSGLSVSPFGNQDFYS
                     FSAIAGSVVTVEIIAQRSPVLGPLDSVIEIMDSNGSRLQTCRDPGNDSPPAPIIPDPT
                     PAAFDDPCVDDDLLLGSINDSKLEFLPSSSGTFFLHVLDFRGDGRPDLQYRIDLSGSP
                     NPLPALDLLSPSTATAGAPGFVLTVSGSGFTSNSVVRWKGMDRTTNFISSTQVQAQIP
                     ASDVAGGDTAQVTVFNPAPGGGTSNALLFTVLNPLPVVGALLPDVIPATSPSFTLRVR
                     GSGFNPSSMVRWNGSDRTTGVDANGDLLAQIPASDVAALGLAKVTVFNPAPGGGTSNA
                     LTFIIIIRNPLPTITSLSPPGVVEDGLGFTLTVNGSNFVFSSEVNWNGSLRTTTFVSQ
                     NQLQAAISGGDILDPGAAAVTIVSPGPGGGTSNAVNFTVRSMPNPLPAGESLNPSSVV
                     AGSVRTTLPLTVNGQNFINGSVVRWNGMDRATVFDSANQLTAMIPANDLVSAGTAQVT
                     VFTPGPGGGVSNAQTFTLNNPVPSLSSLSPGTAAAGNAGFTLLVVGANFVPGSVVQWN
                     GSSRLTTYVGNSQLRALIPASDIAAEGTAMVTVFTPLTGGAGVSNAQTFSITMGGSSS
                     ASSTQEKDAAGSPIAEEPTPSMPVGQAESSLAASWVSTGLSEVAEPPRPEVESLSPRT
                     VEAGSAGFTLIVRGKNFPRGAIIRWNGKERVTRRLSATELRATISREEVAVAGSATVT
                     VYDPRSASESEPLTVVVSPP"
BASE COUNT          907 a         1369 c         1428 g          826 t
ORIGIN      
        1 agcgctttcg cgtcatctac gaccggccgg cgaccttccg ccaagccttc gcgcagttct
       61 tccgacagtc attcgaagtc cgcgagtttg acgccttgaa gggaatatcc ttcgaggtac
      121 gcagcggaga aacggtgggg atcgtcggac ggaacggctc cggcaagagc acgatcctga
      181 agatcatcgc ccgcgtctac cggcccacgg cgggaagcgt ggaagtgttc ggacgggtct
      241 ctccgctcat cgaattgggc gcagggtttc atcccgaatt gacgggtcgg gaaaacatcg
      301 tccttgccgg cgccttgatg ggtattgacc cgagtgacat gcgcaaccgt ctggaggcag
      361 ttgtttcctt ttgcgagctg caagacttca ttgatgctcc tataaagcag tattccagcg
      421 ggatgtatgc acgccttggt tttgctattg ccactgaaat tgatcccgat atcctcttgg
      481 tggatgagat cctggccgtg ggagacgaag ccttccagca gaagtgcctg gagcggatga
      541 acaaattccg gcgagaaggg aaaaccatcg tctacgtctc gcacgacttg aatagcgtaa
      601 agactctctg cagtcgggcg cttctgctcg atagcggcga aatcgtcgag accggcgatc
      661 ctgagaaagt tattgatcgc taccacgaac tgttgcagac atctgccgtg cagtcgtaac
      721 ctgctgaata tacgtaggtt atagctacgg cggggagacg accactgtca gaggctcgga
      781 ctcggaagca gagcgcggat catagaccgt gaccgtcgcc gagccggcga cagctacctc
      841 ttccctggaa atggttgcgc gcaactctgt ggcactgagg cgccgcgtca cgcgctcctt
      901 gccgttccag cggatgatgg cgccacgcgg gaagttcttg ccgcgcacga tgagcgtgaa
      961 gccggcgctg ccggcctcca ccgtccgcgg tgagagcgac tccacttccg gccgcggcgg
     1021 ctcggccacc tcgctcaagc cggtgctcac ccaacttgcc gccaacgagc tctcggcctg
     1081 ccccaccggc attgagggcg tcggctcttc agcgatcggg ctgcccgctg cgtccttctc
     1141 ctgggtgctg ctcgcgctac tactgccgcc catggtgatg ctgaaagtct gcgcattgga
     1201 tacgcctgcc cctccggtca gcggcgtaaa cactgtcacc atcgctgtcc cttctgccgc
     1261 gatatcgctt gccggaatca gagcccgcag ctgactgttc ccgacgtagg tcgtcaaccg
     1321 cgacgacccg ttccactgca ccaccgaccc cggcacgaag ttcgctccca ccaccaacag
     1381 cgtaaacccg gcattcccag ccgcggccgt ccccggcgac aaactggaaa ggctcggcac
     1441 cgggttgttg agggtgaacg tctgggcgtt cgacactcct ccgcccggcc ccggtgtgaa
     1501 caccgtcacc tgcgctgtcc ccgccgagac cagatcgttg gccgggatca tcgccgtcaa
     1561 ctgattcgcg ctgtcaaaca ccgtcgcgcg gtccatcccg ttccaccgca ccaccgaccc
     1621 gttgatgaag ttctgcccgt ttaccgtcag cggcagagtg gtgcgcacgc tccccgcgac
     1681 cacgctgctt gggttcaggg actcaccggc aggcaagggg ttgggcattg atcgaacggt
     1741 gaagttcaca gcattggagg ttcccccgcc cggccccggg ctgacgatgg tgacagcggc
     1801 ggcgccggga tcaaggatat cacccccgga aatcgccgcc tgcaactggt tctggctcac
     1861 aaaggtggtt gttcgcagcg agccgttcca gttcacctcg gaactaaaca caaagttcga
     1921 tccgttcacc gtcaaagtga agcccaatcc atcttccacc acaccggggg gagacaagct
     1981 cgtgattgtg ggcagggggt tgcgaatgat tatgatgaac gtgagagcgt tggacgtccc
     2041 gcctcccggc gctggattga aaaccgtgac ctttgccaga ccgagagccg cgacgtcact
     2101 ggccggaatc tgggccagga gatcgccgtt ggcatccacg ccggtggttc ggtccgagcc
     2161 gttccatcgc accatcgagc tggggttgaa cccggagccc cggactcgca gcgtgaacga
     2221 agggctggta gccggaatca cgtcgggaag aagcgcacca acaacgggaa gaggattcaa
     2281 gaccgtgaac aggagcgcat tcgaagtccc gccgccgggc gcagggttga aaactgttac
     2341 ctgcgcggtg tccccgcccg cgacgtcgct cgccggaatc tgggcctgca cctgagtgct
     2401 gctgatgaag ttggtcgtgc ggtccattcc cttccaccgc actaccgaat tgctggtgaa
     2461 acccgagccg ctcaccgtga gaacaaaccc tggcgcacct gcggttgcag tggagggcga
     2521 aagcaaatcc aaggccggga gcgggttggg cgagccactc agatcaatcc gatattggag
     2581 gtcggggcgc ccatcaccgc ggaagtcgag cacatggagg aagaaagtgc cggaggagga
     2641 gggcaggaat tccaatttgg agtcgttgat agaacccagc aagagatcgt cgtcgacgca
     2701 gggatcatca aaagccgcgg gggtgggatc aggaatgatg ggagcaggcg ggctgtcgtt
     2761 cccgggatcg cggcaggttt gcaggcgact cccattgctg tccataatct ctatgacgga
     2821 atccagtggg ccaaggaccg gcgagcgctg cgctatgatt tccacggtaa ccaccgaacc
     2881 ggcaatcgcg ctgaacgaat aaaagtcctg gttcccgaag ggactgaccg agaggccgct
     2941 ggtcgtcccg ttgaagatgg ccgcggcgcc gcccgggctc tcgttggact ccagagcatc
     3001 gagcgccggc ggagttccgt tcaggacgat gttggcattg gcaaaggtgc caccagcgcc
     3061 cacggccacc tgagtgacgt cgccgggcaa gtccgttgcc gagtcaccga cattgaagaa
     3121 ctcatccggg ccgggattgg ggatgggcgg ggacagaggg cctatgctgg atcctccaaa
     3181 agcgccgggg ttgagggact caacctcaat ggtgtagtcc cccggcggga gacccgggat
     3241 ctcatagaat ccccgcaggg cggggtttcg tgacccgaac cgacttcccg tgctgttgtt
     3301 gccggcaaat ccgctggccg gacacccggt cgacccgggt ggtgaacaag gcagatagtc
     3361 cgcggtgacg gtctgcccag ggttgcccgt aaaagagaag cccgacacca ccgagacggc
     3421 gatccggcgc gattcattct cgggcgttgc cggattgtcg gcttgacgcg caatcacgtt
     3481 gacgccctgg gcgggcgtgc tgccgtcgga aaagaggatc gtaccggtaa tggtgccgaa
     3541 actggaaacg aaccctgcac ccgggtaaag cttcgatacc caggccagat cgtcggtcga
     3601 caatgtttcg gcgtaactca caccgggaga ttcctcctgg tccagcagga aggaatacat
     3661 ggtgggcacg cccaagaggt cgtcggagcc gttggggcag ctcgcgggag taaagtcgca
     3721 attcaagttg atctgcgagt ggtcgagccc gaagaaatgg ccgaactcat gaatgaacgc
     3781 gccgttgaac tggctgggcg ttagctcgag gttgcccatc gcggggtcat tgataccgtc
     3841 aatgaatttg ccgttcagag cagcgtgagc ggagacgatg cggctggggc tggtagagga
     3901 aaccgtgatc gcgcacgctc ccgcaaagcc aatcacaccg gccgaaaacc ccaacgattg
     3961 aaagaggccg ccgttggcat cgtacacaat cgggttttgt tggacggtgt tgcagctacc
     4021 ctcgaccgca ttgaactcgc tgacggtgtc gacgtcaccg cccgcgaatg caccggcggg
     4081 ggctaacggg ccgaggcgat tgaaggccac gttcgaactc gagacgtttt cccatttgtc
     4141 gaacatggcc atgacgctgt tgtttgcggt ggcgttgtcc cgcaggccca ggtttccgcc
     4201 gtcggttcga tactggattt gtcctccaga aaccagggcg ctggtggctc cgctcaagtc
     4261 ccaggtatag gggacgccat ccgccccgaa gccggcgctc cccgcgccga caatgagagg
     4321 cccgcccgct tgggcggagc gctcgaagag ccaactagag acaagcaaac tgcccaggaa
     4381 gagcaaggtc acgagccgcc gggaggttgc gctgcgcatg gctatttccc ggcggcaagc
     4441 gcggcaatga tttcttccag gtcgtccaag ggaaccgggg cggcgcgctc ttcggccacc
     4501 atttgttcca gccgcgaagg caaggcgatg
//
LOCUS       DATIFQ010000411        16085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_137546, whole genome shotgun sequence.
ACCESSION   DATIFQ010000411 DATIFQ010000000
VERSION     DATIFQ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16085
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137546"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="VLE22_20250"
     CDS             <1..1358
                     /locus_tag="VLE22_20250"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB16794.1"
                     /translation="HYFLAVVLMDRREFVEAIAELKKTLELRPSHSEACVALGLCYLR
                     AQNPSGAEEQFRGCLAGKPSSEARYYMGLALGQLGRLEEAAAEFKRAIELNPKSASAH
                     SRLGVTLRRLGDEQGALKEFRIAVRLDDRDPEAWYNLGMALKREEDLSGAIDAFHRAI
                     ERKPDFEQARYNLGIALREQGHQQAAASELREIRGLHEFRVRLAHSKSLVVQGVELLK
                     QQKVDEALAMFQSAADESPELPTAHHYLGLAWEKKGDEARALAAYHKALELKPDYAQT
                     HSRLGVFYWRQNNRPQALEEFRQAVMSDPDIAEPRYNLGLALTELGRLEEAEKELTEA
                     IALDPEYTDARMQLGLVLSRKNDRAGAAGVYREILRRKPGFAEAHNNLGLVLLQDKNL
                     EAAREAFEQALRIRPDYAEAHYNLGLALLEAGREVEARNEMEKAYQLAPEIKKLAPLR
                     R"
     gene            1385..2089
                     /locus_tag="VLE22_20255"
     CDS             1385..2089
                     /locus_tag="VLE22_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HSB16795.1"
                     /translation="MTLLAASLYGAEICEVDRPTAKMSVPRVAKAPALNADPLSDTWR
                     KASAAMIVKDCTRRLDYPDLNTEVRGFWTGSDLYLLFTCPYRELNLFLPAQPGSDRYK
                     LWDRDVVEVFLGDDWKNIRHYREYEIAPTGDWIDLAIDLDRKGDGAKWNSGWETVARI
                     DQAARVWYAAARIPLKSVSKESVRPGTRWRMNLYRIDGKGPDSERRFLCWQPTCVVNR
                     DPNHVPEAFGTLVFAE"
     gene            2098..4404
                     /locus_tag="VLE22_20260"
     CDS             2098..4404
                     /locus_tag="VLE22_20260"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HSB16796.1"
                     /translation="MRTLLFGLLSLSTVQPQTRNAAPRAEDLIEAGRKEFEARCAPCH
                     GGDGRGGERAPAITGRESERVHSEQDLRRVIRMGIPEAGMPPFRLPQDKMDQLAAFVR
                     SLTTPAIERVVPGDVRLGREYFFGTGGCAKCHTAEGRGGVTGPDLSNLGRERTLAQIE
                     RALRTPAASGAPGYKVATVHLRDGQTIRGLIKNESSYDVQLMDLDGRLHLLNAGQIAS
                     IVREEKSVMPAVQANGDEIRNLLAYLSRLSESSLKGDRQAKMLPYSQESFAEGDWPTY
                     HGHPSGNRHSPLRQITTENVARLAPKWIFTIRGSERLEVTPVVVDGVMYVTGVNQAYA
                     LDARTGRLIWSYRRRRTEGVIGDAAGGINRGVALLGDRVFLVTDNAHLLALNRFNGAL
                     IWDVEMADYRQHYGATSAPLIVRDMVVSGTSGGDEGVRGFVAAYSAATGERLWRFWTI
                     PLPGEPLSETWVGRALEHGCASAWLTGTYDPATDLLYWPTGNPCPDFNGDERKGDNLY
                     SDSVLALRPDTGALKWFYQFTPHDLHDWDSQQTPMLVDTVFRGRPRQLLLQANRNGFF
                     YVLDRVTGEFLLAKPFVKKLTWASGIGPDGRPQLLPGSEPTIGGVRVCPSMDGATNWM
                     STAFNPSTGLFYLMALEKCNIFAKSAAWWEPGKSFYGGASRAVEGEVPKKYLRAINVE
                     TGEIVWEYPQIGPGNTWGGVLSTAGGLVFFGDDSGAFAAVDAKDGKPLWNFHTSQVWK
                     ASPMTYMAEGRQYVAVAAGSNIIAFGLP"
     gene            4568..5773
                     /locus_tag="VLE22_20265"
     CDS             4568..5773
                     /locus_tag="VLE22_20265"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB16797.1"
                     /translation="MDSRRNFIGKMATGIAGTLASPAGVLGANDRIRLGIIGPGARGM
                     GIVREAIACPNTEFVAFADVYTRRLEEAKQAVPSAKTHLDYRYLLDDKGIDAVLIATP
                     QHLHCEHFVASLDAGKHVYLEKTMAFTVDHAKRMRASFQRAGGKRVVQIGHQGCSSGL
                     AADARNFVAMGKLGKVTAIHAHHYRNTPHGKPQWSRPVFPDMTSENIIWKSFLGEAPQ
                     RDFDADRFINWRFFWDYSGGNVYENMCHQLAFWYVPLKLQIPKAVTMTGGVYLWKDGR
                     EVPDTMNVSMEHSEEILFSWDSGFGNMHLGSGTYVLGTDGTISNGQQIRFLPEKVNQP
                     DGNEMVGQSKTAPRAHMQDFFDAIRTGKEPNCPFEIGFRVSIACRMAVESYRQGRTVR
                     WDPVKEEIV"
     gene            5814..6986
                     /gene="meaB"
                     /locus_tag="VLE22_20270"
     CDS             5814..6986
                     /gene="meaB"
                     /locus_tag="VLE22_20270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HSB16798.1"
                     /translation="MRDWANRITGGDLRALARAATAVENRTPASRALLDELRPFTGRA
                     LVVGVTGPPGAGKSTLVDALTRALRKEDRTVGIITVDPTSPFSGGAILGDRIRMQSHH
                     ADPGVFIRSMATRGSLGGLARAAADMVALLDGSGRDVVLIETVGVGQDQVEIARLAVV
                     TVVVVIPGTGDDVQAIKAGIMEIADVFAVNKADLPGVERVEQEIRNSLSLAARPDGWT
                     PPVIRTIATEGTGIPELLDAVRSVPRKERPAPPRTEGLRIDHLGIAVPSLDIALAFWQ
                     GQLGVPASPPETVPAEHVRVTMLPAGDARIELLEPSAPDSVIAKYLEQHGPGIHHVAL
                     RVPDLATTVARLRAAGARIVNKPQTGAGGHQYVFVHPASTGGVLLELIQDQEEPSK"
     gene            6983..7831
                     /gene="mtnP"
                     /locus_tag="VLE22_20275"
     CDS             6983..7831
                     /gene="mtnP"
                     /locus_tag="VLE22_20275"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HSB16799.1"
                     /translation="MTAQIGIIGGSGLYSMPGFEAQQEVAVDTPWGAPSDKYVVGRLS
                     GKEVAFLARHGLGHRISPSELNFRANIYGMKTLGVERIISLSAVGSLKEEHRPLDFVL
                     PDQFFDRTRGRVSTFFGEGVVAHISFAHPICPQMADTVHQACVSTGVSVKKGGTYLCM
                     EGPAFSTLAESNVYRSWGMDVIGMTNLQEAKLAREAEICYVTVAMVTDYDCWHPDHDA
                     VTVTDIIANLTKNAENAAKVVAAAVAATPTARNCKCGAALEHAIITDRSLIPESTRKK
                     LSLILK"
     gene            complement(7855..8004)
                     /locus_tag="VLE22_20280"
     CDS             complement(7855..8004)
                     /locus_tag="VLE22_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16800.1"
                     /translation="MPTPAIPRILDPGQKVAEAWKSMKLTLDKDLQATRNGNIAWTTY
                     PEHLT"
     gene            complement(8010..9695)
                     /locus_tag="VLE22_20285"
     CDS             complement(8010..9695)
                     /locus_tag="VLE22_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16801.1"
                     /translation="MIQRKLEGLQEPEEELLECASVAGVKFSPVIVAMVLGRDAEEVE
                     GQCAELASRGTLLRPCSLTEWPDDEVSSWFTFGHSLYQQVLYERIPPSRKARLHRRVG
                     ERLERTFGPSAADRAGELAHHFLAGRDPERALVHLQAAAEAALRKSAPREAAVHLRRG
                     LQLLKTLPETRDHQTREFTLLAMLAPTAIAIEGFAAPDAENGFGRACEIGERLGDSER
                     VSQVILGRAFMHELRGEYYLTEELLGQHLEQAVPQQSPTVAVHADSLMACSLFHQGKF
                     AGALQRAEHALQIYHAGRDREFLAPYGENPSVACQAWAALSLWFLGFPDRAARQVDHL
                     VAFSEAPDHGFTLSTATMYAAHVHQLRRDGACAAQWSERTLRLAQAQGYLYNVSFAKV
                     VKGWADVESGNSEIGLALIREGLAIAESIGAKLDRPYLLALCAEAQLACGRPAAAFAD
                     LKEALSLVRASRAFFYEAELYRLRAAATLRSGAVNAESEAEADLKTALDVARPQGAKS
                     LELRAATDLSALLRKQNRVPAARQILAAPYRLFSEGFQTRDLQEAGTLLDQLG"
     gene            complement(9744..10907)
                     /locus_tag="VLE22_20290"
     CDS             complement(9744..10907)
                     /locus_tag="VLE22_20290"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB16802.1"
                     /translation="MSEIYEFGSFRLDPRERTLTFHSNPIRLRPKVFDTLCVLVACHG
                     KLVTKDELMTLVWPETVVEENNLALSITVLRKTFREVDPEGKWIETVTGKGYRFLGAV
                     RFAGGSSKLGPQGPEQFSRLPGPKLLQRDPELGALRASLNSALQGQRQFVCITGEAGI
                     GKTELLHAFLAGLPSNDGALLLGTGQCVEHSGEGEAYMPVLEALGRICRGTNGTSVVD
                     LLSRHAPSWLVQMPSLVEPAVFSSLQERILGVTRDRMLRELAEAVEAIAERFPVVMAL
                     EDLHWADPSTLELISALARRQQPARFLLIGTNRARDAGESGRALESLTEELHLRGQCN
                     VIRPSLLSEDAIAQIILTQRPDSEGTAQLAATIRERTSGNPLFAHALVDYWSS"
     gene            11036..12373
                     /locus_tag="VLE22_20295"
     CDS             11036..12373
                     /locus_tag="VLE22_20295"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSB16803.1"
                     /translation="MSSSERFPVIATKLEVSLEPGPRLPAGLSYRNDPKSDQTNNLAI
                     TAPKEMKTMTTTINQATINGVNVDALEQSIAAMKDDPRVAAFQFRSSSRWLGGAVIAS
                     TFAGYLQDGLYVARDTPHELGGDEPAALLGTGTQVSPTGHLLHALSHSLAVALAYHGA
                     ARRVEIESIKIDVEGKLDLQGLLGLDETVKPGFKHIHVTFRLDSPNSSAEVYDLFQYV
                     QARSPIASTLSQAVNVQWNFEIEESNSRPESDEVRHGVNVKDLVATVGAIQENPVLAR
                     CNFYGSAEWEGGARVKSSSPGFDQAEGDQIIRHRDPAPKGYVGDEPKALLGSDAGPSS
                     AEALLQSMANCVTVTSSYHAAARGIALDAFEVDFEGDMDLRGFVDVDDTVSPGYQNIR
                     ANVYVKAGAPRSEVEEFMKFATQHSPMCNSVSKPVQLTFSLVHNGSCVGGPGR"
     gene            12450..13874
                     /locus_tag="VLE22_20300"
     CDS             12450..13874
                     /locus_tag="VLE22_20300"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSB16804.1"
                     /translation="MKVQPTAINKLASTLHGRILEPSQPDFDAAREIWNRLYERRPAL
                     VVRCESRSDVAKAVRFAREHDLEIAVKGGGHHVAGHAACDGGLLLDLSRMRGVRIDPQ
                     RRIATALGGCTWADFDPETQEFGLACTGPIVSMTGLPGFLLGGGFGWLHRRLGLGCDH
                     LVAADLVNAEGEYVRATMNHDPELLWGLRGAGWNFGVVTSMELKLDPVGPQVLAGVIY
                     FPIERFAGLTRFHQASMADAPEDLTTWLILRLGPRNPAIPEFLHGQPVCAVGFCHCGG
                     EADAEGWANRIRALPGAFADSITWKPYVDWQRALDGRWGNGFYNDWRSFFFDDLQPEC
                     VEVLLEHMMRLESPLTDIKIPHLGGAIAREPVGGASFGNRRSRYCLVIQGRWSEPAGS
                     DKHLQWSKDLQSALRPFSASGAYVNFLGRDEDSRIVSAYGEEGYRRLAILKDRMDPDN
                     IFHLNPNIGPQSGRVRQANQFHRE"
     gene            14042..14839
                     /locus_tag="VLE22_20305"
     CDS             14042..14839
                     /locus_tag="VLE22_20305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB16805.1"
                     /translation="MEDQVSLSIRQFTEAWRVFCTVSPDHVVADLNGMECIFSGLPIP
                     FFNVVVITERSVSTEALHRHGRAACEWTAGKDVPWFLVVTHETLADGTDAAAVLGECG
                     LAPMVSLTGMLARRVAPAESLPEGLELARPDDDAGCAALLAVNGAAYGIPLDAGIETI
                     GSHSLWKDHVAVVGKVGGKPVSCAAVLMVEEHRYVAFVATDPSHQRRGYADAAMRYAL
                     DLAARAFGETPTALHATDAGRPVYERMGYAPISRHTLFIEKKFLGEH"
     gene            14966..15619
                     /locus_tag="VLE22_20310"
     CDS             14966..15619
                     /locus_tag="VLE22_20310"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB16806.1"
                     /translation="MPASDGEQPIEYGIYTAQDADAMARLLGEVFSRRDPPAVAVGLT
                     APEFEAFVRLYCSKAAAEGLTILARHAGTLELAGALLAEDSALAPPGGMERLSPKFEP
                     IFDILGQLDAEYRCGRPPHPGESLHLFLLGVAESAAGRGVAQHLVTTCLEYGARKGYQ
                     LSVTEATNTVSQHIFRKLGFVERVRRSYSSHRFAGRAMFASIAEQGGPILMDRSLAT"
BASE COUNT         3015 a         5033 c         5055 g         2982 t
ORIGIN      
        1 cccactactt cctcgctgtc gtcctgatgg accggaggga gtttgtcgaa gcgattgccg
       61 agctgaagaa gaccctcgaa cttcgcccct cgcactcgga ggcatgtgtg gccttgggct
      121 tgtgctacct acgggcccag aacccctccg gtgccgagga gcagttccgc ggctgcctag
      181 caggcaagcc ctcttcggaa gcccgttatt acatgggcct cgcgctcggc cagctcggcc
      241 gtttggagga agcggccgcc gagttcaaac gggccatcga actgaacccg aagtctgcct
      301 cggcccactc gcgtctcggt gttacactcc ggcgacttgg ggacgaacag ggggccttga
      361 aggaatttcg gatcgcggtt cggctggatg accgtgatcc cgaggcttgg tacaacctcg
      421 ggatggcgct gaagcgggag gaagatctca gcggcgccat cgacgccttt caccgcgcca
      481 ttgagcggaa acccgacttc gagcaggccc gctataacct ggggatcgcc ctgcgggaac
      541 agggccacca gcaggcggcg gcttccgagc tccgtgagat ccgcggcttg cacgaattcc
      601 gggtgcgcct ggctcactcc aagagtcttg ttgttcaggg cgtggagtta ctcaagcagc
      661 agaaggtgga cgaggcgctg gctatgttcc aaagcgcggc cgatgaaagc ccggaacttc
      721 cgaccgcgca tcattatctc ggcctcgcct gggaaaagaa aggcgatgag gcgcgtgcgc
      781 tggcggccta ccacaaagcc cttgagctca agccggacta tgcgcagacc cattccaggc
      841 tgggcgtctt ctattggcgg cagaacaacc ggccgcaggc gctggaggaa ttccgccagg
      901 ccgtcatgag cgaccccgat atcgccgagc cgcgttacaa cctggggttg gccctgaccg
      961 aactcggccg gctggaggag gcggagaagg aattgaccga agcgatcgcg ctcgaccctg
     1021 agtacaccga cgcccggatg cagctcggcc tcgtcctgag ccggaagaat gaccgcgccg
     1081 gcgccgccgg tgtgtaccgc gaaatcctgc gtcgaaaacc cggcttcgct gaagcgcaca
     1141 acaacctcgg tctcgtcctg ttgcaggaca agaacctgga agccgcccgc gaggcgttcg
     1201 aacaggcctt gcgcatccga cccgattacg ccgaagctca ctacaatctc ggcctcgctc
     1261 tcctcgaggc cggccgggag gtcgaagccc ggaacgagat ggagaaggcc tatcaactag
     1321 ctcctgaaat caagaaactt gcacccttgc gccgatgaac caactctcct gctgtctctc
     1381 cctcatgact cttctcgccg cctcccttta cggggcggag atctgcgagg tggaccgccc
     1441 caccgctaag atgtccgttc cgcgcgtggc caaagcgcct gctcttaacg ccgatcctct
     1501 ttccgatacg tggcgcaaag cctctgccgc tatgatcgtg aaggactgca cacgccgcct
     1561 cgactatccg gatctcaata ccgaagtgcg agggttctgg accggctcag acctctatct
     1621 gctctttacc tgcccgtacc gcgagctcaa cctctttctg cccgcgcagc cggggagtga
     1681 ccgttacaag ctctgggacc gggacgtggt ggaggtcttc ctcggcgacg actggaagaa
     1741 tatccgccac taccgcgagt atgagatcgc gcccaccggg gattggatcg accttgccat
     1801 cgacctcgac cggaagggcg acggcgccaa atggaactcc gggtgggaaa ccgttgcccg
     1861 aatcgaccag gccgcgcgcg tctggtatgc ggccgcccgg atcccgctca agtctgtcag
     1921 caaggagagc gttcgccccg gcacacgctg gcggatgaat ctgtatcgca tcgacggaaa
     1981 aggcccggac agcgagcgcc gcttcctgtg ctggcagccg acttgcgtcg tgaaccggga
     2041 ccccaaccat gtgccggaag ccttcggcac gcttgtgttt gccgaatagg cccacgaatg
     2101 cgcacgctcc tttttggttt gctttctctc tccaccgtcc agccccagac gcggaatgcc
     2161 gctccgcgtg ccgaagatct catcgaagcc ggccgcaagg agttcgaggc ccgctgcgct
     2221 ccctgccacg gcggtgacgg ccgcggcggc gaacgcgcac ccgccatcac gggccgtgag
     2281 agcgaacggg ttcactccga gcaggatctt cgccgggtga tccgcatggg aattccggag
     2341 gcgggcatgc cgccgttccg gctcccgcag gacaagatgg accaactggc cgcgttcgtg
     2401 cgttcactca ccacgccggc catcgagcgg gtcgtgccgg gcgacgtccg cctcggccgc
     2461 gagtatttct tcggcacggg cggctgtgcg aagtgccaca cggccgaagg ccgcggaggc
     2521 gtgaccggtc cggacctctc gaacctcggg cgtgagcgta cgctcgctca gatcgaacgg
     2581 gcgctccgaa cgccggcggc ttccggcgcc cccggctaca aggtggccac ggtccacttg
     2641 cgcgacggcc agaccattcg cggcctcata aagaacgaaa gcagctatga cgttcaactg
     2701 atggacctgg acggccgcct gcatttgctg aacgccggcc agattgcaag cattgtgcgg
     2761 gaagaaaaat ccgtgatgcc ggccgttcag gccaacggcg acgagattcg taatctgctt
     2821 gcgtatctca gccggctctc tgagtcatcc ctgaaaggcg accggcaggc gaagatgctc
     2881 ccttactcgc aggagtcctt cgccgagggc gactggccca cctatcacgg ccatccgagc
     2941 ggtaaccgcc acagccctct ccgtcagatc acaacggaaa acgtcgccag gctggcgccg
     3001 aaatggattt tcacgattcg cggctccgag cgtctcgaag tgacaccggt tgtcgttgat
     3061 ggcgtgatgt atgtgaccgg cgtcaatcag gcgtacgccc tcgacgcccg gactggccgt
     3121 ctgatctgga gctaccgcag gcgcagaacc gaaggcgtga tcggcgatgc ggccggcggc
     3181 attaaccgcg gcgtcgcgct gctcggtgac cgtgtctttc tggtgaccga caacgcccat
     3241 ctgctcgcgc tcaaccgctt caacggcgcg ctcatctggg acgtagaaat ggccgactac
     3301 cgccagcatt acggcgcgac ctccgcgcct ctgattgtga gagatatggt ggtatccgga
     3361 acctccggtg gcgacgaagg agttcgcggc ttcgtcgctg cctatagcgc ggctaccggc
     3421 gagcggcttt ggcgcttctg gacaataccc cttcccggcg agccgctgtc ggaaacgtgg
     3481 gtcggcaggg cgctcgaaca cggttgtgca tcggcgtggc tcaccgggac ctatgacccc
     3541 gcaacagacc tgctttactg gcccacgggc aatccttgtc ccgacttcaa cggcgacgag
     3601 cgcaagggcg acaacctcta ctccgactcc gtactcgccc tacggccgga tactggtgcg
     3661 ctcaaatggt tctaccagtt caccccccat gacctgcacg actgggattc tcagcagacg
     3721 cccatgctgg tcgacaccgt cttccgcgga cggccgcgcc agttgcttct ccaagccaac
     3781 cgcaacggct tcttttatgt gctggaccgg gtgaccggcg agttcctgct ggcgaagcca
     3841 ttcgtcaaga aactgacctg ggcgagcggc atcggccccg acggccggcc tcagcttctg
     3901 ccgggcagcg agcctaccat cggcggggtc agggtttgtc cctccatgga cggcgccacc
     3961 aattggatgt ccacggcctt caatccttcc acggggctct tttatctgat ggctctcgag
     4021 aagtgcaaca tcttcgccaa atcggcagcg tggtgggaac cgggtaagtc attttacggt
     4081 ggcgcttcgc gcgcggtcga aggcgaggtt ccgaaaaaat accttcgtgc catcaacgtc
     4141 gaaaccggcg aaatcgtgtg ggagtacccg cagatcggtc cgggaaacac ctggggcggc
     4201 gtgctgtcga ccgctggcgg cctcgtattc ttcggggacg acagtggagc gttcgccgcc
     4261 gtcgatgcga aagacggcaa gccgctctgg aacttccaca ccagccaggt ttggaaggcg
     4321 tcgcccatga cgtacatggc cgagggccgc cagtacgtcg ccgtcgcggc cggctcgaat
     4381 atcatagcct tcggcctacc gtgatagtac agcgggggga caggcttact ccgccccgag
     4441 cgcactttgc gagtgggcgg agctgcccgt cccccgctgc ccacctggcc tgactcccat
     4501 gcggctcccc ggtgctaaga tgaaggagcc gcagcatcag cagcatacgt ttgacgattg
     4561 gcatcctatg gattcccgcc gcaatttcat cggaaagatg gcgaccggta ttgccggaac
     4621 tctggcctcc cccgccggcg ttctcggcgc caatgaccgt atccgcctcg gcatcatcgg
     4681 acccggcgcc cggggaatgg gcatcgtccg cgaggcgatc gcctgtccga acacggaatt
     4741 tgtcgccttc gccgacgtct acactcgccg cctcgaggag gcgaaacagg ccgttcccag
     4801 tgctaagacg catctcgact accgttatct gctcgacgac aaaggtatcg acgccgtcct
     4861 catcgccact ccgcagcacc tccactgcga gcacttcgtc gcctcgctcg acgccggaaa
     4921 acacgtctat ttggaaaaga cgatggcttt caccgtcgat cacgcgaagc gcatgcgcgc
     4981 gtcctttcag cgcgctggcg gcaaacgcgt cgtccagatc ggccaccagg ggtgcagctc
     5041 cggactcgcc gccgacgcca gaaacttcgt ggctatgggc aagctcggca aggtcaccgc
     5101 gatccacgcc caccactacc gcaacacgcc ccatggcaag ccccagtggt cgcgccccgt
     5161 cttccccgac atgacgtccg agaacatcat ctggaagtca ttcctcgggg aagccccgca
     5221 gcgcgacttc gacgccgacc gtttcatcaa ctggcggttc ttctgggact actcgggtgg
     5281 caacgtctat gagaatatgt gccaccagct tgcgttttgg tacgtcccgc tgaaacttca
     5341 gattccgaag gccgtcacca tgaccggtgg cgtctacctc tggaaggacg ggcgcgaagt
     5401 gcccgacacc atgaacgtct ccatggagca ttcggaggaa atcctcttct catgggattc
     5461 cggcttcggc aacatgcacc tcggctccgg aacgtacgtg ctcggcacgg atggcaccat
     5521 cagtaacggc cagcaaattc gcttccttcc cgaaaaagtg aatcagccgg acggcaacga
     5581 aatggtcggc cagtccaaga ccgcgccgcg cgcccatatg caggacttct ttgacgcgat
     5641 ccgcaccggc aaggaaccga actgcccgtt cgaaatcggt ttccgcgtct ccatcgcctg
     5701 ccgcatggct gtcgaaagct accgccaggg acgaaccgtg cgctgggatc cggtgaagga
     5761 agagatcgtc tgattggcgg cgggaaccgc accgtccgtt tctcatcgag ctgatgcgcg
     5821 actgggcaaa cagaatcacc ggcggcgacc tgcgcgccct cgcgcgcgcc gctaccgctg
     5881 tcgaaaatcg cacgcccgcg tcccgcgctc ttctcgacga gctgcgcccg ttcaccggac
     5941 gcgccctcgt tgtcggggtc accggaccgc ccggcgccgg caagagcaca ctagttgacg
     6001 ccctcacccg cgcgttgcgc aaggaagacc gtacagtcgg catcatcacc gtcgacccga
     6061 ccagcccgtt ctccggcggc gccatcctcg gggaccgcat tcgcatgcag tcgcaccacg
     6121 ccgaccctgg cgtcttcatc cgctccatgg ctacccgcgg ctcgctcggc ggccttgccc
     6181 gcgccgccgc ggacatggtc gcattgcttg acggctccgg ccgcgacgtg gtgctgatcg
     6241 aaaccgtcgg cgtaggccag gatcaggtgg aaatcgcccg gctggccgtc gtgaccgtcg
     6301 tggtggtgat tccggggacg ggcgacgacg tgcaggcgat caaggccggc atcatggaga
     6361 tcgccgacgt cttcgccgtc aataaggccg acctgcccgg cgtcgagcgt gtggaacagg
     6421 aaatccgcaa ctccctctcg ctcgccgccc gcccggacgg ctggacgcct cccgtaatcc
     6481 ggacaatcgc taccgaaggg accggcatcc ccgagttgct ggatgccgtg cgttcggttc
     6541 cgaggaagga acggcccgcg cccccgcgaa cggaaggcct ccgcatcgat cacctcggta
     6601 tcgccgtgcc ttcgctcgac atcgccctcg cgttctggca gggacaactc ggcgtgcccg
     6661 cctcgccgcc tgagaccgtg ccggcggagc atgtccgggt aactatgctc cctgcgggcg
     6721 acgcgcgcat cgagctgctg gagccctccg cgccggattc ggtgatcgcg aagtatctcg
     6781 aacagcacgg ccccggcatt caccacgtcg cccttcgcgt gccggacctc gcgacgactg
     6841 tggcgcgcct gcgagccgcc ggcgcacgca tcgtgaataa gccacagacc ggtgccggcg
     6901 gccatcaata cgtctttgtg caccccgcct ccaccggagg tgttttgctc gagctgattc
     6961 aggaccagga ggaaccatcg aagtgacagc gcagattggc attattggcg gcagtgggct
     7021 ttactccatg cccggttttg aagcgcagca ggaggtggca gtcgacacgc cttggggcgc
     7081 gccgtccgac aagtacgtcg tcgggcgtct ctcgggcaaa gaggtggcgt ttctcgcccg
     7141 ccacggcctc ggacatcgca tctctccctc cgaactgaac ttccgcgcca acatttacgg
     7201 catgaaaacg ctcggcgtgg agcgcatcat ttctctcagc gctgtcggct cgctcaagga
     7261 ggagcaccgg ccgctcgact tcgtgcttcc ggaccagttc ttcgaccgca cccgcggccg
     7321 cgtctccacg ttctttggtg aaggcgtcgt ggcgcacatc agcttcgcgc atcccatctg
     7381 cccgcagatg gcggacacgg tgcaccaggc gtgcgtctcg accggcgtca gcgtgaagaa
     7441 gggcggcacg tacctttgca tggaaggccc ggcgttttcg acgctcgccg agtccaatgt
     7501 ctatcggagc tggggcatgg acgtgatcgg catgaccaat ttgcaggagg cgaagctcgc
     7561 ccgcgaggcg gagatttgct acgtcacggt tgccatggtg accgactacg actgctggca
     7621 cccggaccac gatgcggtta cggtgaccga catcatcgcc aacctgacga agaacgccga
     7681 aaacgccgcg aaggtggttg ccgccgccgt ggccgcgacc cccacggcac gcaactgcaa
     7741 gtgcggcgcc gccctggagc acgccatcat cacggatcgc tccctcatcc ccgaatccac
     7801 ccgcaagaaa ctctccctta ttctcaaata ggtcctaatt tcgaggcggg caacctaggt
     7861 caggtgttcc ggataggtgg tccacgcgat gttgccgttg cgagtggctt gcaagtcctt
     7921 gtccagagtc agtttcatgg acttccaggc ctccgcgacc ttctggcccg gatccagaat
     7981 tcggggaatc gcgggagtcg gcacgattgt cagcccagtt gatcgagtaa ggttccggct
     8041 tcctgcaagt ctcgagtctg aaaaccttcg ctaaagagcc tgtaaggcgc tgcgaggatt
     8101 tgtctggcgg ctggaacacg attctgtttc cgcaacaacg cagagaggtc cgttgcggct
     8161 cgcagctcaa gagatttggc cccctgtggg cgagctacgt ccagggcggt cttgagatct
     8221 gcctcggcct cgctctccgc gttcaccgca cccgagcgca gcgtggctgc tgcccgtaga
     8281 cgatacagtt cggcttcata gaagaaggct cggctagccc tgaccagcga gagtgcttct
     8341 ttgaggtcag cgaaagccgc tgcaggccgg ccacaagcta gctgcgcttc ggcgcacaag
     8401 gctagcagat acggccgatc cagcttggct ccgatggatt cggcgatcgc caggccttca
     8461 cggatcaagg caagtccaat ctccgaattt ccgctctcca catcggccca gcccttgacc
     8521 accttggcga aggagacgtt gtagagataa ccctgcgctt gggccaggcg gagcgtcctc
     8581 tcggaccact gggcggcgca ggcgccatcc ctgcggagct ggtggacgtg tgcggcgtac
     8641 atggtggcag tggacagggt aaacccatgg tcgggcgctt cgctgaaggc gaccagatga
     8701 tcgacttgcc gcgcggcacg gtccggaaat ccgagaaacc acaagctcag agccgcccac
     8761 gcttggcagg cgacggacgg attttcgccg taaggggcga ggaactcgcg atcgcgtccg
     8821 gcatggtaga tctgaagggc gtgttcggcg cgctgcaatg cgcccgcgaa cttgccttgg
     8881 tggaacaggg agcaggccat gaggctatcg gcgtgaaccg cgacggtggg actctgttgg
     8941 ggtacggctt gttccaaatg ctggcctagg agctcttcgg tcagataata ttcgccgcgc
     9001 aactcatgca tgaatgcacg gccgaggatg acttgggaaa cacgctcgga gtcgccgagg
     9061 cgttcgccta tttcgcaggc ccgcccgaaa ccgttctcgg cgtcgggagc ggcaaagcct
     9121 tcgatggcga ttgcggttgg ggcgagcatg gcaagcaagg tgaactcgcg cgtttgatgg
     9181 tcgcgggttt ccggcagcgt cttcaagagc tgcagacctc ggcgcagatg aacggcggct
     9241 tcacggggag cacttttccg cagggcggcc tcggccgcag cctgaagatg gaccaaggca
     9301 cgctcggggt cgcggccggc gaggaaatgg tgcgccagtt cgccagcccg gtcggcggcc
     9361 gagggtccga acgtgcgttc caaacgctca cccacacggc gatggagacg cgccttccgg
     9421 ctgggcggaa ttcgctcgta taggacctgc tgatataggg agtgcccgaa ggtgaaccac
     9481 gacgatacct cgtcgtccgg ccattcggtt aggctgcagg gccgcagtag tgttccgcgg
     9541 gaggctagct ccgcgcactg cccctcaacc tcctctgcat cgcggccgag aaccatggcg
     9601 acgatcaccg gcgagaactt cacacctgct accgaagcgc attccagaag ttcttcctcg
     9661 ggttcttgta agccctccag cttgcgttgg atcattgccg ccaggtttcc cggaacgccg
     9721 cggggcagct cgctgccggc tccctacgaa gaccaatagt ccaccagcgc gtgtgcaaac
     9781 agagggtttc cagaggtgcg ttcgcgaatc gtggcggcca actgcgccgt cccttcggag
     9841 tcaggcctct gcgtgaggat tatttgcgcg attgcgtctt cgctgaggag gcttggccgg
     9901 ataacgttgc actgtccgcg aagatgaagc tcttctgtaa gcgattccag cgctcttccg
     9961 gactcgccgg catcgcgcgc gcgatttgtt ccgatcagga gaaagcgcgc tggttgttgg
    10021 cggcgtgcca gggcgctgat gagctcgagc gtggaggggt ccgcccagtg aagatcctcg
    10081 agagccatga ccaccgggaa gcgttccgcg atggcttcta cagcttcggc tagctcgcgg
    10141 agcatccggt cgcgggtgac gccgaggatg cgttcctgga gactgctgaa gaccgcgggt
    10201 tcgactagcg agggcatctg caccagccag gaaggcgcgt ggcgtgagag aagatccaca
    10261 acgcttgtgc cgttggtgcc gcggcagatt cttcccagag cttcgagcac gggcatgtaa
    10321 gcttcgcctt cgccggaatg ctccacgcat tgtccggtcc cgagcagtaa ggcgccatcg
    10381 tttgacggca ggccggcgag gaacgcgtgc agcagttcgg tctttccaat gccggcttcg
    10441 ccggtgatgc aaacaaactg acgctgtccc tgcagggcgg agttgagaga cgctcgtagg
    10501 gctccgagtt ccgggtcacg ctggagcagt ttcggcccgg gcaagcggct gaactgttcc
    10561 gggccctgcg gtccgagctt cgaagagccg cccgcgaagc gcacggctcc gaggaagcgg
    10621 tagcctttcc cggtcacagt ttcgatccac ttcccttcgg gatcgacttc acggaaggtc
    10681 ttgcgcagca cggtaatgct tagcgcgagg ttgttttcct caaccacggt ctcgggccag
    10741 accagcgtca tcagctcgtc tttggtcacc agctttccgt ggcaggccac cagaacacac
    10801 aaggtgtcga acaccttcgg gcgcagccgg atggggttgc tgtgaaacgt cagagtccgt
    10861 tcccgaggat cgaggcggaa tgagccgaac tcatagatct cactcattct caggaacata
    10921 gtaagccaaa ggaaacttaa agaaaagttt gagacgactt taacgacctg cgggggcctg
    10981 ttgcagcaga atcggattgt cggttgggac actcagccgt gcaaaggaga aagcgatgag
    11041 tagcagcgaa cgatttccgg tgattgcgac gaagcttgaa gtatcgctgg aacccggacc
    11101 gcgactcccg gcagggcttt cctaccggaa cgatcccaag tccgaccaaa ccaacaacct
    11161 ggccattacg gcccccaagg agatgaagac aatgacgacg accatcaatc aagcgaccat
    11221 caacggcgtg aacgtggatg cactggaaca aagcatcgcc gccatgaaag acgatccgcg
    11281 cgtggctgcc ttccaattcc ggagctcgag ccgctggctg ggcggggcgg tgatcgcctc
    11341 caccttcgcc ggctatctgc aggatggcct gtacgtggcg cgcgacacac cacacgagct
    11401 gggcggcgac gaaccggccg cgctgctagg cactgggact caggtgagcc cgactggtca
    11461 ccttctgcac gcgctcagcc actcgctggc ggtcgccttg gcctatcacg gagcggctcg
    11521 gcgtgtggaa atcgaatcga tcaagatcga tgtggaaggc aagttggacc tgcaaggtct
    11581 gctgggtctg gacgaaacgg tgaaaccggg attcaagcac attcacgtga ctttccgcct
    11641 agattcgccg aactcgagcg ccgaagtgta cgatctgttt caatacgtgc aggcccgctc
    11701 accgatcgcc tcgaccctga gccaggcggt caacgtgcag tggaatttcg agatcgagga
    11761 gagcaactcc cgtccggaat ccgatgaagt gcgccacggc gtaaacgtga aagacctggt
    11821 cgcaaccgtg ggcgcgattc aggagaaccc ggtccttgcc cggtgcaact tctacggttc
    11881 cgcggaatgg gagggtggag ctcgcgtcaa atcgagcagc ccgggtttcg accaggcgga
    11941 gggcgaccag atcattcgcc atcgtgatcc tgcccccaag gggtacgtgg gtgatgaacc
    12001 caaagcgctg ctgggctctg atgccggtcc gtcatcggcg gaggcgctgc tgcaatcgat
    12061 ggcgaactgc gtcactgtca ccagttctta ccacgcggcc gcccggggca tcgctctgga
    12121 cgctttcgag gtggacttcg aaggcgacat ggacctgcgt gggtttgtcg atgtcgatga
    12181 cacggtatcg cctggttacc agaacatccg ggcgaacgtg tacgtcaagg ctggcgcgcc
    12241 acgctcagag gtggaggagt tcatgaagtt cgcaacccaa cactcaccaa tgtgcaacag
    12301 cgtgtcgaag cccgtgcaac tgacattttc tctggttcac aacggaagct gcgtaggggg
    12361 tccgggaaga tgaccgtcga ttgctgcggc gccgtgtttc cagccggtgc cgcagctctt
    12421 gcccgatctt gtgaaaaggg gagcgaacga tgaaggtcca acccactgcg atcaataaac
    12481 tggcgtccac gctgcacggg cggatcctcg agccttcgca gccagatttc gacgcagcac
    12541 gcgaaatctg gaatcggctg tatgagcgac ggccggccct ggtggtccga tgcgaaagcc
    12601 gctcggatgt ggccaaagcg gtacgcttcg ctcgcgagca tgatcttgaa atcgcggtga
    12661 agggaggagg acatcatgtg gcgggacatg ctgcttgcga tggcggtttg ttgctggacc
    12721 tgtccaggat gcgaggggtc cgcatcgatc cacagcggcg aatcgccacg gcgctcggtg
    12781 gctgtacctg ggccgacttc gatccggaga cccaggaatt cggccttgcc tgcactgggc
    12841 cgatcgtttc catgaccggc ttgcccgggt tccttctggg gggtgggttc ggatggttgc
    12901 atcgcaggct cggactcggc tgcgatcatc tggtggccgc cgatctggtc aatgccgagg
    12961 gcgagtatgt gagagcgact atgaaccacg atcccgaact gctatggggg ttgcggggag
    13021 cagggtggaa tttcggtgtt gttacttcga tggagttgaa gctcgatcct gtcggcccgc
    13081 aagtactggc cggggtgatc tatttcccga tcgaacgctt tgctgggctg acgcgatttc
    13141 atcaggcctc gatggcggac gcgcccgagg atttgacgac atggctaatc ctgaggctgg
    13201 ggccgcggaa tcctgcgatt ccagagttct tgcacgggca gccggtctgc gcggtcggct
    13261 tctgccattg cggtggagag gcagatgcag aaggatgggc aaaccggatt cgtgccctgc
    13321 ccggtgcgtt cgccgactcg atcacgtgga agccgtacgt cgactggcag cgggccttgg
    13381 atgggcgatg ggggaacggg ttctacaacg attggcggtc gttcttcttc gatgaccttc
    13441 agccggaatg tgtcgaagtc cttttggaac acatgatgag gctggagtca ccgctcaccg
    13501 acatcaagat tccccatctg ggtggagcca tcgcgcggga gcccgtggga ggcgcgtcct
    13561 tcggaaatcg ccgcagccgg tattgtcttg tgatccaggg cagatggagc gaacctgccg
    13621 gctcggacaa gcatcttcag tggtctaagg atcttcagtc cgctctgcgg cctttcagcg
    13681 caagcggagc gtatgtgaat ttcctgggac gggatgagga cagccggatc gtgtcggcgt
    13741 acggggaaga ggggtatcgg agactcgcga tactgaaaga taggatggat cctgacaaca
    13801 tcttccactt gaatccgaac atcgggcccc aaagcgggcg agtcaggcag gcgaatcagt
    13861 tccatcgtga atagcagcgt attagtaatg gacacgatcc gtgctcgtag ttgggtaacg
    13921 gacgaaccgg agattgccgg gatcgggacc gccaagcctg ctgtccgaat ccccgacgat
    13981 cggcctgctg agcaggacta aagagaaaag gatataattc attccacttg agaggcggcg
    14041 cgtggaagat caggtatcat tgagcatccg gcagtttacg gaggcatggc gtgtgttttg
    14101 cacggtgtct ccggaccatg tggtagcgga ccttaatggg atggagtgca tcttcagtgg
    14161 tctgccaatt ccatttttca acgtcgtcgt gatcacggaa agaagcgtct caactgaagc
    14221 gctgcaccgt cacggcagag cagcgtgcga atggaccgca gggaaggatg tgccctggtt
    14281 tctcgtcgtc acacacgaaa cgctcgcgga tggtacagac gcggcggcgg tcctcggtga
    14341 gtgcgggctg gcgccaatgg tgtcgctgac cggcatgctg gcccggcggg tggccccggc
    14401 tgagagcctg ccggagggat tggagttagc ccgaccagac gacgacgcgg gctgtgcggc
    14461 gctcttggca gtgaacgggg cggcgtatgg cattccgtta gacgccggca tcgaaacaat
    14521 tggctcgcac agtttatgga aggatcacgt cgccgtagtg ggaaaggtgg gcgggaagcc
    14581 cgttagttgc gccgcggtcc tgatggtgga agagcatcgt tacgtcgcat tcgttgcaac
    14641 ggaccccagc catcaacgac gcggttatgc ggacgcggcg atgaggtacg ctctcgatct
    14701 ggccgcccgc gcctttgggg agactcccac cgcgctccac gccacggacg cgggccgccc
    14761 ggtgtacgaa cgcatgggct acgcgccgat ctccagacac acgctcttca tcgagaagaa
    14821 gttcctgggc gagcattgac cggattccca gccagcgacc gggcacggtc acccgtgcgg
    14881 tgcacagaaa tgccgaccgc atgcggtctt ggagttggcg cggttcccgc ctggcgaacc
    14941 cagaaccaga agaaggaggc aaccgatgcc agcttctgac ggcgagcagc caatcgagta
    15001 cggcatttac acagcccagg acgcggatgc gatggctcgg ctgctgggtg aagtgttcag
    15061 ccgaagggat ccgcccgcgg tggcggtcgg gctgaccgct ccggagttcg aggctttcgt
    15121 ccgactgtac tgttccaaag cggccgccga gggcttgacc atcctggcgc gacatgcggg
    15181 cacgctggag ttggccggtg cgttgctagc cgaggatagc gcgttggcgc cgcccggcgg
    15241 gatggagaga ctgagtccga agtttgagcc tatcttcgac atcctgggtc agctcgatgc
    15301 cgagtatcgc tgcggccggc ccccgcaccc cggcgagtcg ctccacttgt ttcttctcgg
    15361 ggtggcggaa tccgccgcgg gccgcggtgt agcgcagcat ctcgtcacca cctgcctcga
    15421 gtacggcgcg cgcaaaggct atcaactctc tgttaccgaa gccacgaaca cggtgtcgca
    15481 gcacattttc cggaagctgg gatttgtcga gcgcgtgcgg cggtcctact ccagtcatcg
    15541 cttcgccggg cgtgccatgt ttgcgtcgat cgccgagcag ggaggcccta tccttatgga
    15601 caggtcgctc gcgacctgat gggccacgca cactggatct gccagaccgc ccaactggcc
    15661 atggttcaaa gccggttatc tgctgttatc gctcggcact gccagcggcc gccacacgga
    15721 aatcgcgggt gctccgcgct caagctgtca gtcccgcctc accgaaggcg actagaatgg
    15781 aagtggccgg cctgctgccg cccctaataa tccgatcttt tcggcgtcac ctgggctgga
    15841 tttttctagg cgagctgaaa tctttggcag gtagcgtgga gcccgctccg ttctccgatc
    15901 acaacgcgat ctgggtccac gttagcctgt agtaccgcct caggcggccc gctcccgcac
    15961 ctcgccggac ttctttccac tgtcctgccc ggcccaccgc gctcggatac actgagatca
    16021 ccgattacga acggagagaa tttgcagacc atgtccgctc tcaagaagat tctcctgccg
    16081 gtcga
//
LOCUS       DATIFQ010000412        88884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2630966, whole genome shotgun sequence.
ACCESSION   DATIFQ010000412 DATIFQ010000000
VERSION     DATIFQ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 88884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88884
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630966"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2265
                     /locus_tag="VLE22_20315"
     CDS             <1..2265
                     /locus_tag="VLE22_20315"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSB16807.1"
                     /translation="KGRSDPTAQPEVRLIRIHEGKTTTLASGLGTFLAGAIATAAKPD
                     PSGRRLALVPITANNKLAVIDLASGKPAGTLPTELVPFGAVLSRDGAVAYVSNWGGRP
                     PKPGDLTAPLGLRPDADRAVVDERGIASTGTVTRIDLKAMKATHTIPAELHPTALAWD
                     EQRNRLYVANANTDSISVIDTQTQTVVAAFPLVPFAVKAFGIAPTALVLSPDGAQLYV
                     ACGGINAVMLLEAGTGRIEGMIPTAWYPNGLALSSDGKLLAVTSLLGAGSGWRDEPRK
                     RFVHSYRGALNVIEIPDKAQLASYTTVVSENNRLPLAGSASERPAQPAVDAKPVAIPA
                     RAGELSLIEHVVFIIKENRTYDQVFGALPQGNGDPSLVMFGRDVTPNHHKLAEQFVVI
                     DNFYATGGNSADGHQWLTQANEVAYCLWPGYAGRSYPFDGSDPLAYSHSGFLWDAALA
                     RGRTVRVYGEYAGRLPVPSSERQPLLERWRNGEDFTQRWNITAPIDKLNTILARNYPT
                     YTTHIPDVVRAQIFLADLRKLEQEGRMPNLVMIQLPSNHTNGAQPGISSAKAMVADND
                     LAMGKIVEALSHSQFWPKMAVFIVEDDAQNGVDHVDGHRTVALVASPYAKRGAVDSTF
                     YSTQSMVKTIELILGLPTLSLFDLIAHDMRNSFTDTPDLTPYKAEEPTQSLFEQNPPV
                     EGLKGPAREAAVASMKMRFDVPDAAPSDRLNRILWHQVRGWNTPYPGTRTAVFAPLSL
                     DLDDDEREEAVGRE"
     gene            complement(2441..4138)
                     /locus_tag="VLE22_20320"
     CDS             complement(2441..4138)
                     /locus_tag="VLE22_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16808.1"
                     /translation="MSIRESRFGRLVQHFFAGFLDNDLISSPETGMHAAFTQILGLVA
                     APGCFYCLLMLLKYAAVDYPEREIITWPDKILFIGFSMILAGLLSVIEWDALFPDRRD
                     YQVLVPLPITTRLLFAAKLSSVLLLLGILWAAVNLGPAVMFPAIVGSVTKSSFDYGRF
                     VAGHIIATFAACSFVFLFFVGLQGIMLNILSPKWFRRLSVYIQLATVLALVLLFLLMP
                     MLLFSVTARVHGNDPWMYAFPPFWFLGLYQELMGRGSPVFSPLAKVAVAAITVCAAVA
                     ALAYWISYRRHMKRLLESLEPGRSGPGFMARLAAVLLNHFVLTHPLERGCFQFIRLTL
                     LRSRVHRLLMAAYVGVGLALVLGSAAVILRYSAGGRPVPAVLSVQLVLSFFVVSGMRF
                     AFTVPAELNANWLFQAAEPSETARCRAGVRRAMLALAIYPVLLGLLPAHVLLWGWRVA
                     GAHLAYGLVLSALLVELLLLNFDKIPFTCTYLPGKANVKGFWPVYLAAYWMYAHYMAK
                     VEYRLLDRPASFALVLSAIAALTVLLRWLRLRQLGPDFRFLFEDLPDPAVRTLDITH"
     gene            complement(4151..4918)
                     /locus_tag="VLE22_20325"
     CDS             complement(4151..4918)
                     /locus_tag="VLE22_20325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB16809.1"
                     /translation="MLEVHGLTKHYSAIPAVDDVSFTIHPGEVLGYLGPNGSGKSTTV
                     KMITGLLDPTRGHVYFHGRDIKDDLPGYKRSVGYVPEEPHIYPYLTGREYLLLVGRLR
                     CLPEKALGEKADALLRLFGLQSDRHSLMASYSKGMRQKVLICGALLHDPELLVFDEPL
                     SGLDVTTALVFRDLVRALAGRGKIILYSSHVLETVEKVCSRVVILKRGRIAADGDVLE
                     LRDLLQAPSLESVFAQLVLQESTEGIASDIAEAMRIS"
     gene            complement(5285..5749)
                     /locus_tag="VLE22_20330"
     CDS             complement(5285..5749)
                     /locus_tag="VLE22_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16810.1"
                     /translation="MGVGVALVLGGAAVILLSASGGKPAPALLSVQLVLSFFVVSGMR
                     FAFTVPAELHANWLFQAAEPSETARCRSGVRRAMLALAVCLVLLALLPAHALLWGWQF
                     AGAHLAYGLVLSVLLIELLLWNFEKIPITCSYLPGKANVKGFWPLHLGMYWI"
     gene            complement(5776..5916)
                     /locus_tag="VLE22_20335"
     CDS             complement(5776..5916)
                     /locus_tag="VLE22_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16811.1"
                     /translation="MLDVHGLTNHYSAIPAVDDVSFTIYPGEVLVVIFKRGHVAADWG
                     RP"
     gene            complement(6009..6692)
                     /locus_tag="VLE22_20340"
     CDS             complement(6009..6692)
                     /locus_tag="VLE22_20340"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HSB16812.1"
                     /translation="MRISEVFYSTQGEGALVGTPSVFVRTSGCNLRCVWCDTPYTSWQ
                     PEGTEMEVGEILADVRRYPARYVVVTGGEPMIAPEIVPLTEGLRTAGLHITIETAGTV
                     FAPVACDLMSISPKTSNSTPRKRDGGRWAAQHDRLRFQPQVLARLMSDYDYQLKFVVQ
                     QPEDLNEILAMVTDLGASRLKVVLMPEGTDPATLHERSLWIAEICKREGFRFSPRLHV
                     DLWGARRGV"
     gene            complement(6689..7384)
                     /gene="queC"
                     /locus_tag="VLE22_20345"
     CDS             complement(6689..7384)
                     /gene="queC"
                     /locus_tag="VLE22_20345"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HSB16813.1"
                     /translation="MSERPKAVCLLSGGLDSSACLAFARKEGFECYALSFDYGQRHRA
                     ELEAAARVAADLGAARHLVVSFDLRAIGGSALTADIEVPKARPAEVIGLGIPITYVPA
                     RNTIFLSFALAWAEVLGASDIFIGVNAIDYSGYPDCRAEYIEAFERMAVLATKAGVEG
                     NTRVRIHTPLIRLSKAEIVRFAAALGVDFRLTVSCYDPDERGRPCGACDSCLLRLKGF
                     QQAGLKDPLEYRS"
     gene            complement(7381..8577)
                     /locus_tag="VLE22_20350"
     CDS             complement(7381..8577)
                     /locus_tag="VLE22_20350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB16814.1"
                     /translation="MSSEANVAEVSSHSAAERAARAGVATLVLMSLGHFFVDLYSGAL
                     GALQPLLVDRFGTNLTQAGILGGLLVFFSSVMQPVYGFLSDRYHTRMFTVLAPATAGI
                     FISSLGLAPSYVWMLPMVVLGGTGIASFHPQASARVAFGSTDRRGIAMAIFISAGALG
                     YALGPTFFSGITGALGLSRSYWAAVPGVVLTLLLFPLLPPAPSSMTHPRNRFDWKVLR
                     AVRKPLTILYVLVFIRSIIQITFGQFLPLYLHLEKGFPVSRANYALSGYLAAGALGGF
                     LGGHLADRFGGRTVILLSMAGSLPFLALFFFGSGWASLLGLILGGFILLFTIPVNVVM
                     AQELAPTQAGTVSALMMGFAWGTAGLIFIPLTGWASDLFSMHNVLASLLVFPVVGFFL
                     TLKLKK"
     gene            complement(8677..10791)
                     /locus_tag="VLE22_20355"
     CDS             complement(8677..10791)
                     /locus_tag="VLE22_20355"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HSB16815.1"
                     /translation="MVTPTVMRTFHAAMLITVFLASSSCRQGPKPPVAKIMPTKLEKH
                     GHVRTDNYFWLKERDKPEVIDYLKAENQYTEAIMAHTADLQAKLFDEFKTRIKQTDES
                     APYRKDGYYYYSRVEQGRDYPIYCRRKGSPDAPEQVLIDVNQIAAGHGFCSVPPPAVT
                     TSGAIMAYAVDLVGRRFFDVRFRNLETGEEYRDVIRNITGNVVWAEDNKTLFYSRQHP
                     ETLRSYQIFRHVLGADPAGDTLVFEEKDETFSTYVLKTKSKKYILIASRQTLSAEYRF
                     LDAAKPAGEFRVFLPRQRDHEYQIDHFGDQFYIRTNLQAKNFRLMKTPVARTAVANWK
                     EVLPHRADVFFEDMEIFKDHLVVSERKAGLIQLRVMPWSGQGEHYIDFGEPVYVAATS
                     QNYELDTPLVRYDYSSLTTPNSVYDYNMVTREKKLVKRDEVLGGFDPTNYQSERLFAT
                     ARDGTQVPISLVYRKGFRKDGKAPLLLYGYGSYGISTDPNFSAMRISLLDRGFAYAIA
                     HVRGGQEMGRSWYEDGKLLKKKNTFTDFIDCADYLVKEKYTSPERLFAEGGSAGGLLM
                     GAIFNMRPDLFKGVVAQVPFVDVVTTMLDDSIPLTTAEYDEWGNPNEKPYYDYMLSYS
                     PYDNVAAKHYPNLLVTTGLHDSQVQYWEPAKWVAKLRAMKTDKNRLLLKTEMEAGHGG
                     ASGRDKRYKEIAFIYAFLLDLV"
     gene            complement(10823..13213)
                     /gene="lpxK"
                     /locus_tag="VLE22_20360"
     CDS             complement(10823..13213)
                     /gene="lpxK"
                     /locus_tag="VLE22_20360"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HSB16816.1"
                     /translation="MRKISHLDDKLIYSLYRAAQWLGFPFIVLYFLGRGLRDRRYFRR
                     FGERLGFIAHSCKRPAPGAIWIHAVSVGEVLSAPGLLQGLRRSFPTAPLFVSTTTLAG
                     RALADEKLASVVCGVFYAPIDYCFAVRRVLRTIRPAVVVVLETEIWPNLYREAKRAGC
                     TLIVVNGRISDRAAPRYHRLRWFFRPVLRQADRILVQTEVSLRRYLELGAPAVLGGNL
                     KYDFEPPSGGAAPEVRKALDRLKPGSIWIAASTMPPAEPGDPDEDDSVIAAFCELAPS
                     RPRLLLIHVPRRPERFDSAAEKLTAAGVPFVRRTALADQPPLPALPCVLLLDSIGELS
                     ALFAHADVVFMGGTLPHRGGHNILEPAFFARPVVIGPHMENFPEIAAEFIEAEACVRI
                     DSPLELAAAVDTLLSDPDRRSLLGERARKLAEARRGATDRAVREIEQLRSRALPVYRP
                     AFPLYQILWLLSGLWWLGGLAKRAIDTARRRRLSTPVISVGSITMGGSGKTPFVLWLA
                     ERLKDSGLSPAILTRGYRRRVPEKHTILGPGDQVSPARTGDEAQIFLRSGVGPVGIGA
                     GRAAVGRLVEERFRPDVMLLDDGFQHWRLARDLDIVLIDALAPFGPNQHLFPLGRLRE
                     PMGALSRAAAFVMTRTEPGRGYDAIVDRLREYNRAAPVFFSRVVPKSWVEAGTVGQFD
                     PRGLPHTRLGAFCGLANPSSYWSTLTALEIEPLARWTFGDHHTYKPVDLRRLTTQART
                     AGIEALLTTEKDAMNISEGALALLSPLKLYWLDITVEVDGAIELLSLIRSKLFRAS"
     gene            13212..13775
                     /locus_tag="VLE22_20365"
     CDS             13212..13775
                     /locus_tag="VLE22_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16817.1"
                     /translation="MSIVAAFGADKDKFDPGPVTSLAARQTIGKVTIGAREFDTEELA
                     RTAFGKLNPNKYGVLPVLVVIQNDGDKTLSLDRIHFQYLVPGHDEIDATPATDVKYIG
                     GASKPRTGPSPLPIPRGKKKSPLTAWEIEGRALAARMLPPGQSASGFVYFQAASLQGA
                     KLYVTGIRDAGSGQELFYFEIPFDGGK"
     gene            complement(13833..14522)
                     /locus_tag="VLE22_20370"
     CDS             complement(13833..14522)
                     /locus_tag="VLE22_20370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB16818.1"
                     /translation="METSRWIALSLLLLLTLNLAAQTAQAPTPPAQAAREDGLYATFK
                     TTQGDITVKLYEKESPITVRNFVSLAQGRKAWKDPKSGQMVRKPLYNGLTFHRVIPGF
                     MIQGGDPQGDGAGDVGFVIPDEFHPSLKFDQPGRLAMANAGPKTGSCQFFITEVPTPH
                     LNGAHTIFGQVIQGQDVVTKIANVPRGANDKPRTPVRITSVVIERVGAAPVPPPAAKK
                     AAPRKAVPKKK"
     gene            complement(14532..15650)
                     /gene="mqnE"
                     /locus_tag="VLE22_20375"
     CDS             complement(14532..15650)
                     /gene="mqnE"
                     /locus_tag="VLE22_20375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HSB16819.1"
                     /translation="MQPIIDDARLRPILDKVAAGERLSFEDGVTLYRSPDLLALGYMA
                     NLVRERRHGNVTYFNVNRHINPTDVCVASCRLCAFGKRVKDERAYTMSLEQVWETAGR
                     GWSEAITEFHIVGGLHPELTLDWYCEAFRGLKQRFPQVHLKALTMVEIAYLAHRTKIT
                     AGETLRRLKDSGVDSLPGGGAEIFSERVRRIICDHKIDGSQWLDVARQAHRIGLQSNC
                     TMLYGHIETEEDRVDHLMKLRELQDETGGFVTFIPLAFHPDNTPLRHIPKTTGFDDIR
                     NIAVSRLMLDNIPHIKAYWVMMTPRIAQIAQRFGSDDVDGTVVEEKIYHDAGATTTQS
                     LRRGELLRLIREAGREPAERDTLYRPVVRSETTFTVLV"
     gene            complement(15654..16547)
                     /locus_tag="VLE22_20380"
     CDS             complement(15654..16547)
                     /locus_tag="VLE22_20380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HSB16820.1"
                     /translation="MLRRFWNRLWLTLDMIRFEHSVFALPFALTGALLAVREDGFATP
                     HLGRKVFWIVVAMVAARSAAMAFNRILDAPMDARNPRTAARHLPAGVLSRRFAWGFTA
                     AASAVFIVAARALNSLCFYLAPAALAVVFFYSFTKRFTSLSHLVLGFSLGIAPAAAWI
                     AIRGSLDPRILWLTAAVTLWTAGFDIIYACQDFEFDGREGLFSLPRRFGIAGALWISR
                     LFHLAMIGCLAALVYAFSLGALSLAGLGAVALLLAYEQSLVRPGDLSRVNAAFFTVNG
                     YVSVLFFLFWAADIFLLRQGT"
     gene            16642..17094
                     /locus_tag="VLE22_20385"
     CDS             16642..17094
                     /locus_tag="VLE22_20385"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSB16821.1"
                     /translation="MRRTIKTAIACAALALAGCGSKTALDEYYTQVVTLPNGKEIRAE
                     VMADRTDMMRGMMFRDSLAPDRGMLFIHDRPDRHTYWMYQVRIPLDIVWLDGNRRIVE
                     ISANTPPCKSQASKCPQYGGKEMSQYVLELAGGVAAQHGLRLGEQLTF"
     gene            17139..17858
                     /gene="larB"
                     /locus_tag="VLE22_20390"
     CDS             17139..17858
                     /gene="larB"
                     /locus_tag="VLE22_20390"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HSB16822.1"
                     /translation="MEQVRAGGVGIDAAVERMRHMPFEDLGFAKVDHHRALRHGMPEV
                     IFAQGKTPEQVVAIAGRLLKSARNILITRVDERTAALVKRKIRKVEYFPLSRAIRVWQ
                     DRTIRGKGKIAVVCAGTSDIPVAEEAQVTAEVMGNEVIAIHDIGVAGIHRLMSNRERL
                     TEAKVVIVAAGMEGALPSVVGGLVSCPVIAVPTSIGYGASFNGLAALLAMLNSCASNV
                     TVVNIDNGFGAGYVASLINRV"
     gene            complement(17880..18317)
                     /locus_tag="VLE22_20395"
     CDS             complement(17880..18317)
                     /locus_tag="VLE22_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16823.1"
                     /translation="MAAEYETLKKRVLREATVLPNFLVPETVVREDGRGPDSELGDLQ
                     GRLLLLTLGITRIIEQESLDISVWGSADNADWGTKPLLSYPQKFYCGTYQVLLDLNDR
                     PEVRYLRVQWKVSRWGRGASKPLFGFYVFAEEMGRQALTAKTA"
     gene            complement(18407..19504)
                     /locus_tag="VLE22_20400"
     CDS             complement(18407..19504)
                     /locus_tag="VLE22_20400"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSB16824.1"
                     /translation="MTRLAIRHKAVNLAQGMPDFGAPGELKEAAREAIAAGINQYAIT
                     WGARPFRAAIAAKYRHWYGIDVDPEREITVCCGATESMIASLLAVTNPGDEVVIFEPF
                     YENYGPDTRLSGAVPRLVKLRPPDWTFDPDELRRAFGPRTKAIILNTPNNPTGKVFTR
                     EELEFIARLCQQFDALAITDEIYEHILYDGARHVPIMTLPGMRNRSILVNSMSKTYSV
                     TGWRVGWALAAPDLADSIRKVHDFLTVGAAAPLQQAGVTALSMPDEYYETLARDYAVR
                     RDRTLSILAGAGFHCFTPKGAYYVMTDVSGFGFPDDVAFVRHLIEHAGVAAVPGSSFF
                     QDPRDGARMVRFCFCKKPETLDEAARRLARL"
     gene            complement(19563..21770)
                     /locus_tag="VLE22_20405"
     CDS             complement(19563..21770)
                     /locus_tag="VLE22_20405"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSB16825.1"
                     /translation="MRTLALTFLLLFPLVLPAQNLESLVRACRERPSVAGRDAIARFA
                     AAHPKDSQGAMALFALGVIDFEDGSYPEAIKRLSEARKRLPQLDDYVAFHLGASHLEA
                     GDPAAAASELRTVLEATPPSPLTSRAAILSAKAYTESGAPTDAITVLRAGYARLTQPA
                     GDFALASALESAGDLPAAAGYYQRVYFQFPATSAASAAASALERLRAAMGAGYPPPMP
                     HAMLERAGKWLEAREYGRARDEFEGLIPRLGGLERDLASVRIGVADYMAQRTQTAYQY
                     LKSLGSLSPEAAAERLYFMTACARRLDDTQAMQEHLAELGRLHPQSEWRLKALVWAAN
                     EYLLRNDVAAFEPLYRACYESFPASAEASNCHWKVAWAEWMRRTPAAPVLMAEHLSRY
                     PESPKASAALYFLGRSAEEAGRSGEARALYRKVVEHFPNRYYAFLCEDRLTQGPLARN
                     TGSNMPPTAFDGLQLPVRGGPVSFKPGEQSRLRIARARLLASTGLSDFADAELRFEAR
                     ANGDAPSLALELARQASRRGVPETAIRHIKGVWPTYLYTPLESAPAEFWRLAFPLPYR
                     QSLEKYGRLRNLDPFVLAGLIRQESEFDRTAISRARAYGLMQVLPSTGRYLARKTRSG
                     RFRSALLFRPDYNIRLGTYYFRQRLDELGGDVEAALAAYNGGKSRVDVWTTWGPFREP
                     AEFIETIPFTETRTYVQVVLANAEVYRRLYGPKATEVPSTDGAFRKRAVAGKK"
     gene            complement(21775..22878)
                     /locus_tag="VLE22_20410"
     CDS             complement(21775..22878)
                     /locus_tag="VLE22_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16826.1"
                     /translation="METPRLLPWTDFSLTSTLEEQRITAREQLKAAWQLHVERVEEQL
                     TAGWKEQIDRVLEDRFAELASLVEEEFGREISARIAGQRETLRRETAEQLNQAARRMR
                     QSESGDDLHAVLLDAADAFCDRAVLFAVEGVALRCAGARGLDPDAAGVLLVAETPLEA
                     SPAAAGAVESLDTVVAMRTAGELSETLASALGEDSAGRSFFFPVVCQGKAVGLLYADG
                     HETRLHTAALELLTALAGGALDSRRAAATAASSGLVMIAGQAPGPSSKLPDWPALPPE
                     DQDLHLRAQRFARVQVAEMRLYKPREVRSGRLSSNLYADLRGDIDAGREAFRLQFAVS
                     CPTMVDYFHLELVRTLANDDPALLGPEYPGPLV"
     gene            complement(22919..25267)
                     /locus_tag="VLE22_20415"
     CDS             complement(22919..25267)
                     /locus_tag="VLE22_20415"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HSB16827.1"
                     /translation="MKLPRVTLRKVVLAALMLAAAAVAVAPFLHADQFRRRVHQSLEA
                     ALGRKVEIADVRFSLLRGPGFTLNKVVIHEDPAIGIEPIAYVASMEANIRIGALFTGQ
                     LEFSSLRLNEPSVNLVKTVAGPWNFQPLVARAAAADLPEFRVRGGRLNFKFGDTKSVF
                     YFTGADLDIDPPSVPGGRFDVRFSGEPARTDRPAHGFGRFSGRGRWQPSPPGDGRLDL
                     SLNLERSAIGELVTLVHGEDLGVHGRVAATARLRGPIDNVEIAGDLQLQDIHRWDLMP
                     PYAAGGPLRFRGRVDLKSQRLEIETVPERNSALIVRFRASRYLSDPAWGVNVALNRLP
                     VASVLEISRHMGFSFTDTLSAEGEMTGVFGYSHADGLQGSADMLGARIGLVGSPPAVF
                     EEARLTVAGSRLRLAPALLRTADGETVRLDLDYQQDPERVDLQVQTQQLSLGGPDRGQ
                     SLPAGIGRVPFLDHFRGGAWSGALRYRQNPGTPGEWSGAIRVTGTNVEVNGLAAPLAI
                     EAASVKLDGGRLSVTDVKGALGETAIQGDYRHQPGARYPHRIRCRVERLRAGELEWLF
                     MPTLQRQAGLLSRALAFGRSPLPEWLRARKAEGTLEIGNFAVQGLDFESVRARWFWDG
                     ERLEVTRVSARVAQGRIDGHLSIGVGGREPVYDLGVAVDSIAWRGGTLGGDARLRTSG
                     SGDELLRRMHAEGTFQGRSVALSPDTELPLVSGCYSALWSGKGPSVELDCLRVAVGTD
                     VLTGHAVAREDGRISVELSGGAHPLRLAGSLSPLELRLEPPR"
     gene            complement(25264..25866)
                     /locus_tag="VLE22_20420"
     CDS             complement(25264..25866)
                     /locus_tag="VLE22_20420"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSB16828.1"
                     /translation="MKLAALILAAGASSRMGTPKALLPLAGETFAERLARLFREAGCA
                     PVILVLGHDAETVRHGLAPEVADAVVVNPDYARGQLSSLQSGLSAVPEDADGFLFTPV
                     DHAGVRPSTVSAIVRRFSGRSGELLVIPQSNGRRGHPVCCARELIPEFLSLAAGATAR
                     DVVHRHLDRTARVEVNDPGIHEDADDPEAYRRLLSAPLRP"
     gene            complement(25863..26699)
                     /locus_tag="VLE22_20425"
     CDS             complement(25863..26699)
                     /locus_tag="VLE22_20425"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HSB16829.1"
                     /translation="MDIFEELVRLRREGRRCALATIVQVQGSIPSYESAKLLIRDDGS
                     IAGTIGGGCVEAEVWNAAREVIESGKAKRLSFNLGQDAAYDNGLICGGQLEVFVEPVF
                     PQPKAFIFGAGHISKSLAKVAALAGFSTAVIDDRESFASRERFPEADEIHAGEYEDVV
                     PRLAINESGYVVIVTRGHRDDMRVLRLVAGSSARYIAMIGSKRKVISVIKELEREGVA
                     RESLERIYAPMGLEIGAESPEEIAISVVAEMIAVRRNAPSDWRELSKSIFAHENTRAL
                     LK"
     gene            26717..27445
                     /locus_tag="VLE22_20430"
     CDS             26717..27445
                     /locus_tag="VLE22_20430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HSB16830.1"
                     /translation="MPEHRQFRNRVCYHPLRVKQAGGLASGLALVFDLDGVVVDSNPV
                     HTETWRLYLERHGVEADEDMPWRMFGRRNDDIVRDLFGPGLSQEEVIEHGAAKERLFR
                     EEMRDQLPHRLVPGLVAFLERHRDAPLGVASNAEPENVAFVLDCAGLRHYFNVVVDGH
                     QVQKPKPSPDIFLKAAELLETAPGNCVVFEDSLAGVEAAIAAGMRVVGVCTTHGSLPG
                     VQLEIEDFRSIELESWLRRQIPLL"
     gene            27448..29742
                     /locus_tag="VLE22_20435"
     CDS             27448..29742
                     /locus_tag="VLE22_20435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HSB16831.1"
                     /translation="MAGLTAILLVGIPAAGQTPLTLEQAASREGSDFRPLYEGREVIL
                     RGRVSVQVIPVVHYAHLAIQDDTGHGFTIDGPAEELARFSPGDVIVVRGKVSERAGLP
                     VLNPAEIRIIGKGPVVEPRRVPVEQLSGFRHQGVYVVTEGLVRGVGTNEGGHFLLLGN
                     SGAPVKVFLPDAASTSSGGLTQFRVGDRVRVTGISSQYAPLPPFNRYFQLMIPGAGSV
                     ELVDRPWPIPIWALISTLGLLATAVGIWSIRERHMAARRRAMRRLYQLGEEMVGAVSP
                     EESLRILNATLPETLGIVSVRMYLYSHSEKRLRPVVEAGAAPSPGLLLEEAATCWGNR
                     SLLLIPDVRRSPFHAEGAAGLPRAALFLPMFAQKEPVGVLEVGDARRGREFSADELAV
                     AQHLANQIAIGIELRERHSIRDELFRSEKLAATGQLISNVAGELKAPLEEIEKAVHRL
                     RGSRVEVEIISSQASRAAAIVDRLLEFTRKDDESTRFDLAQVLQTLLKNREGAWTEKS
                     VRFENLLSSDPIFVTGSGKQLEEVLLSLVKHAEESALRTAAKIVTVRKTKLVRGVQIE
                     ILFSDPGDTGADPFSETNGADGEALGLGVCRSILRGQGGDLRVFRQPDGFLRFEVELP
                     AGPSANGDSATVSAPGDTRITTLILEPDVDSQRRLTALLSEGGHRTVPTVTAEDALEI
                     AHRFPFHAIFCSTRLAGMDCVELLERTRDRIDAFVFLSESYDGESVHALPHGEGYVLT
                     KPVTSEDLDRVLRSIQEKVAARSS"
     gene            29801..30289
                     /locus_tag="VLE22_20440"
     CDS             29801..30289
                     /locus_tag="VLE22_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16832.1"
                     /translation="MVSRTALTAVFLAFFPALNFAAESYNGPKPPKPDVAFLLHADNL
                     VETETLEAKEETRKDETIYTIPGAASSVRTPLAEPIFLIQVEKIVPEKLSLFRLEVKE
                     GNRRVVFNQKKRKDNPEPIRFIATEIEGKLYRIEVNQPLENGEYSLSPAGSNQVFCFQ
                     VY"
     gene            30344..30718
                     /locus_tag="VLE22_20445"
     CDS             30344..30718
                     /locus_tag="VLE22_20445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB16833.1"
                     /translation="MFKGLEHTAIASPNPERLAQWYVDHLGFIINHRYDGNVFVKAKN
                     GGMLEIIPSEGDRAPQKLKDPGLRHLAIAVDDFEAAHRQLRSLGVEFATDVMNLKGNL
                     LVFFLDGDGNYVHLIHRPAPLP"
     gene            30731..31936
                     /locus_tag="VLE22_20450"
     CDS             30731..31936
                     /locus_tag="VLE22_20450"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB16834.1"
                     /translation="MVQRVFKAFQYRDFRLMWLGACTSSVGTWMQKLAQSWLVLQISG
                     SPFLLGLDSFLGEIPIFLFSLIGGVVADRTDRRHLLLASQFVQLSCAFLLAGLIVFDV
                     VKVWHILTLSFVVGLAQAFGGPAYQALIPSLVRSEDLSNAIALNSIQFNLARVIGPML
                     GGLALTKLGAAWCFGLNGVSFVAVIISLLMLQIRFVPERTNESMLTSMKQGIQFIRRQ
                     RAMEPLIALAFLMTLLGIPLIVFLPVFAKDVFHHGANTYTLFLSISGAGSVLGALVVA
                     GMGNIRHKGRTALLMLVALGICMGGFALSKSLVLSCLLLFTAGIALISVFSLVTSLVQ
                     LIAVDEMRGRVMSVYNVAFRGGMPIGSLVTGSLVPVFSAPSVFACNGVLLTALGLYFL
                     LVQRRVASL"
     gene            31948..32631
                     /locus_tag="VLE22_20455"
     CDS             31948..32631
                     /locus_tag="VLE22_20455"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB16835.1"
                     /translation="MRAAAIIGTLLLAPWLVASELEAARDMQDAAKLEALIAPLADAA
                     ARSTNDAPAQYRLALARSYLAEVAMELRDKTRAKVAAEAGIQAAERAVALAGNVAEHH
                     RILGTLCGQVIPANVLFGLKYGRCATDSVNKAIELDPKSSSAYLSRAVGNYYLPEGLG
                     GGAQLALRDCQKAIELNPKSAEAHLWLGIMLRKLNRNEEARTAIRRSLELNPRRVWAK
                     QQLEKTPTK"
     gene            32631..34292
                     /locus_tag="VLE22_20460"
     CDS             32631..34292
                     /locus_tag="VLE22_20460"
                     /inference="COORDINATES: protein motif:HMM:NF042614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HSB16836.1"
                     /translation="MRKSLPVAAALILLTAVGYLEFPGHTYLLSDTQIYIPILEHYRD
                     PSLFTREMVAVRPHVAFTIYDEMALGLRHFTGLGFEQVLTIQQLLFRALGILGVFLIA
                     TSMGLSRQMAFVVSSVFTLGATIGGPTVLTFEYEPIPRGFAVPLLLLAAGMVAHGRDF
                     GAGIAAALAFLYHPPTVYTFWGVYFLLTLWPNKPAVMSRRIWGLLPMLCAVVVLLFLS
                     RLQAGVTEAQPFFSRISPELEKLQRMRAPYNWVSLWFPYWHWLSVFLWAVSLAAYWRV
                     RRWAPEDLRFFLVGLPLLGILSLPLSHVLLEKLAWILIPQAQPARAVLFVTVFAGILA
                     SVAGVKAVEAGRRIEGVIWFLLAFAIPIHTRVTELLLPDLSEPVIRRRLLLAVAFSGV
                     AVLAAWAASVGRKWAPAAMVLVSLAPFWLIPSYGNVVTYREVRTPELLELCNWARQET
                     AKDAMFLFPNAGRSLEPGIFRATALRAVYVDWKAGGQVNFFPKLGFEWWERWRQTMAR
                     PFRPEKLPLFASLGIDFIVLPAKNRLKDGAPVYSNAGFVVYALRK"
     gene            complement(34308..35243)
                     /gene="rbsK"
                     /locus_tag="VLE22_20465"
     CDS             complement(34308..35243)
                     /gene="rbsK"
                     /locus_tag="VLE22_20465"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HSB16837.1"
                     /translation="MKPSLVVVGSLNMDFVVQVEHLPAPGETVLGRDFQMIPGGKGAN
                     QACAAGKLGGPAVSVRMAGRVGYDLFADHLKASLSAAGVDVTAVHATKSQPTGVALIW
                     VDRSGQNSIVVASGANHALLPNDVGAMRQSFRGARFVLFQLETPLDTVELALVAAREE
                     GARTILDPAPARPLSREFLAAVDILTPNESEACHLLGRPVTRVSQADAPALAHDLQAL
                     GPRTVILKLGEQGCFCLDGERGTQAHSFPVEARDTTAAGDTFNAGLAVALAEGKPMEE
                     ALRFANGAAAISVTRLGAQASVPSRREVEEFLLSR"
     gene            complement(35240..35827)
                     /locus_tag="VLE22_20470"
     CDS             complement(35240..35827)
                     /locus_tag="VLE22_20470"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HSB16838.1"
                     /translation="MLLPRELIFYLSRQLVTKLSPQVIETTNPQAATELVSSVITDEL
                     QVEDRLNDEVREILSQYSDYMRREGVSYQEMFRRIKNTLISQRKVVRASGRDTGDRMK
                     LSRDKINEISHKLISALRKAREIRVKRDANDVRLEIVQQITELLQVEDRVDRAARLKI
                     KSQKREIQEGTEEWDLLHRRYYADEMKKLGIDLSR"
     gene            36038..37024
                     /locus_tag="VLE22_20475"
     CDS             36038..37024
                     /locus_tag="VLE22_20475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HSB16839.1"
                     /translation="MERGSSTWLAVAKAAMDVEASAISLAARRLGENLCQAVELILDR
                     PGKVVVTGVGKSGQVAQKLAATLCSTGISAVFMHAGDAVHGDLGVLAPGDPCLLVSKS
                     GATAELIRLVPVLRQVQCPLIGIIGDANSPLARDVDVLLDASVSREADPENPVPTAST
                     AVAMALGDALAVALMHARNFTVDDFGRHHPGGQLGRNLRLQVKAAMHCGEEVAWAHRD
                     DSLKAVVIAMTRRPVGAACVVREDGRLEGLITDGDLRRALEAHDDIRGLRAADVMSGP
                     PVVVSPDARLADALALMENRPRQISVLPVVEPASGRCLGLLRLHDIYQVGNA"
     gene            37017..37871
                     /gene="kdsA"
                     /locus_tag="VLE22_20480"
     CDS             37017..37871
                     /gene="kdsA"
                     /locus_tag="VLE22_20480"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HSB16840.1"
                     /translation="MPEVTSASAPAPITLADASARTVVFGGGRPLALIAGPCVIESEE
                     HVHFLAQQISSIAGPFVFKASFDKANRSSLSSYRGPGIKEGLRILGALKAAGHFILTD
                     IHEPDQAAQAAEVADIVQVPAFLCRQTDLLLEAGRTGRIVNLKKGQFVAPHDFRLAVE
                     KVASTGNTKILLTERGVCFGYNNLVVDMRGLVIMRSFGFPVIFDATHSVQLPGGAGSS
                     SDGQAQFIEPLARAATAVGIDGLFVEVHNAPEKALSDGPNALRLNLLGALLERLRSID
                     ALVKTQRP"
     gene            complement(37868..38311)
                     /locus_tag="VLE22_20485"
     CDS             complement(37868..38311)
                     /locus_tag="VLE22_20485"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HSB16841.1"
                     /translation="MATRLFTVQEAESILPAVEADLRQAVSIKPQYADAEAELNAVVQ
                     RVTMSGGAVVNREQLIHVRGRRDALAARLKELINAIQEFGCLVKDLDNGLLDFPTLFR
                     DEEVYLCWKLGEPGIGYWHRIQDGFRGRQRIDADFLANHRGEPPN"
     gene            complement(38324..38830)
                     /locus_tag="VLE22_20490"
     CDS             complement(38324..38830)
                     /locus_tag="VLE22_20490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HSB16842.1"
                     /translation="MPTTNTETPADATIEPNGGRTGVQTGYRVFSRIVDWITTERMEL
                     LNVTDRVNEIVRKSGIRDGLVHLQSLHTTTAVFMNEWQDALRHDVKTFLSQMVQHDQY
                     WRHNDPEHSDCERSNADSHLRGMLLGQTLCLQVRNASVLLGTWQSIIFAEFDGPRTRS
                     LAVQVSGI"
     gene            38963..39454
                     /locus_tag="VLE22_20495"
     CDS             38963..39454
                     /locus_tag="VLE22_20495"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSB16843.1"
                     /translation="MDAINALKTRRSVRAYRREPIPREALTDLIDCARLAPTAINIQP
                     WEFVVVTERETLNSIAGTTDHGRFIADAAACVVVLCQDTKYFLEDGSSATLSILLAAH
                     AHGLAGCWVAGDKKPYAAEVCRIVGAPANYRLISLVPIGYPAEQPEKEKRPLSDVLHW
                     EKF"
     gene            39556..40506
                     /locus_tag="VLE22_20500"
     CDS             39556..40506
                     /locus_tag="VLE22_20500"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HSB16844.1"
                     /translation="MSNKRTIAVVGATGGQGGGLVRSILGDPNSGFTARALTRDVSSA
                     KAKALEKLGAEVVSADVNDTESLKKAFSGAHGAYCVTFYWDHFSPEKEMAHAAAMAQA
                     AKDTGVKHVIWSTLEDTRKRVPLSDGRMPTLMGKYKVPHFDAKGEANHFFTDLGLPVT
                     FLLTSFYWDNLIHFGMGPKTGSDGKLEITMPMGDKKLPGIAVEDIGKCAHGIFKKGQD
                     LIGKTVGIAGEHLTGSQMAAALTKALGREVRYNDVPPEVYRSFGFPGADDLGNMFQFK
                     RDFETEYCGARNPEVARSLNPSLQTFAMWLAEHKSRIPLE"
     gene            40603..42033
                     /locus_tag="VLE22_20505"
     CDS             40603..42033
                     /locus_tag="VLE22_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16845.1"
                     /translation="MRLARGFSPAAGTLILCLCTARALSGQAPAPSNRITIDPAKQFQ
                     VIEGFGLNYTGPYFRNDQKPMFDMLVKDLGASMFRVVAYFVYSDWESTNDNDDPHSAN
                     WEYYNSRYSNPVFEATWNGLRHLNSMGIRPVLALMGPVPDWMVEEGTPPPQHKVCQSS
                     SKLGRLKPEMYDEFAETVVTMAAYARRKAGVDFDLFSPFNETDCYPPEGPRVDPDEVA
                     KVLTAVARRMNQEGLGDVRLAVADHAIITTDYISPVLKDADLMKQVGAFTVHTYGEDS
                     VGPHVKNVARSAFRNVPVWLTEYGDLNDQDQTFENEWKRYSLGANRRALTALNQGAGA
                     LFYFNAFDDYEECARRLTYYGLFRSAGQVYSPKKRYYATKQLYHFVKPGARRIAVTTD
                     AQGVTISAFQDGESDSLVVVGVKQGGPNRLKITLPAPSRAPRSWDLYMTTHLLDCRKV
                     QTIQNAGPSLEIELPDEAVFTLTGRF"
     gene            complement(42061..43011)
                     /locus_tag="VLE22_20510"
     CDS             complement(42061..43011)
                     /locus_tag="VLE22_20510"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HSB16846.1"
                     /translation="MSSDFLCSDGRPLFSRRTFVGTLAGLSASGAPKKRIVFIAGVKT
                     HGYGEHSHNAGCLFLAKRLNESLPGVEAIVHRDGWPAEPSYFDNASAVVIYMDGGDKH
                     PMLPHLEALDALMKKGVGLAVLHYALVAPEGEPGDKLLQWIGGYYETYWSVNPIWTAR
                     FERLPEHPVTRGVRPFTMRDEWYYHMRFRENMDGVTPILTAVPPDATRQGPDGPHSGN
                     PVVRSRKGMAEHVAWAYDRPGGGRGFGFTGAHYHWALADDNYRRVLLNGIAWIAGVDI
                     PRDGIASKTPSWKELFETAEGAMPADFSREKAEQAIHPRK"
     gene            complement(43055..43222)
                     /locus_tag="VLE22_20515"
     CDS             complement(43055..43222)
                     /locus_tag="VLE22_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16847.1"
                     /translation="MNRHLEDLPAGDGFVMETGTIRNLRPRRRTPSPGTPADLAINVN
                     LTSMPWFGVGR"
     gene            43314..43793
                     /locus_tag="VLE22_20520"
     CDS             43314..43793
                     /locus_tag="VLE22_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16848.1"
                     /translation="MREQFAQKKRSGRFPSTAPQNANDHRSGKGDSNYDIRQTMTTNG
                     VWMLPFGPGQRFLQSQGFAGKLVGRWELSAFHAARTGRATNFSISRSSKDLPDGNSSN
                     QRPDVVPGVSIYPEHQTLDNWFNIAAFAVPAKGTYGSPRFCCACLCLAGAKPVCDGH"
     gene            43780..44139
                     /locus_tag="VLE22_20525"
     CDS             43780..44139
                     /locus_tag="VLE22_20525"
                     /inference="COORDINATES: protein motif:HMM:NF014396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HSB16849.1"
                     /translation="MTAIEPAVATAPVGEDPDDPAVWVNPSDPARSLVLGTNKTGAPQ
                     GALYVFDLKGKMLQKISNLDRPNNMDIEYGLVLNGQPTDIAVLTERYRQRLRVFRIAS
                     DGSGLMRYFLGRRHARV"
     gene            44216..44623
                     /locus_tag="VLE22_20530"
     CDS             44216..44623
                     /locus_tag="VLE22_20530"
                     /inference="COORDINATES: protein motif:HMM:NF014396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HSB16850.1"
                     /translation="MSAGRLGPKQGYLWQYRLEDDGVGLVKGTKVREFGRFSGTGEVE
                     AITVDDAVGYVYYADEGDGIHKWHADPDHPMATRELAHFGRKGFKADREGIAIYARPD
                     GTGYLLCTDQLPGTSEYRVYRREGKAGRPHDHS"
     gene            complement(44810..46105)
                     /locus_tag="VLE22_20535"
     CDS             complement(44810..46105)
                     /locus_tag="VLE22_20535"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB16851.1"
                     /translation="MAGFRRLLGQNRNYRYTWMGQVVSEIGDHFNNIAVFSLAVERTG
                     SGMVVTGIMLSRGLAVVLAGPLAGVLLDRMDRKRIMIISDLLRAAVALMFIFTVSRPE
                     PWLLYVLSGLLMFASPFFTSGRASILPTIASREELHTANSLTQTTQWTTLTIGTMTAG
                     LSAAHLGYEWAFFLNFLSFLFSAWAISSLRMPKGGFRSKRTALTETEVVRPWHEYAEG
                     LRYMRASPLILGIALIHVGWATGGGAAQILFSLFGELVFKRGAAGIGILWSFAGLGLL
                     VGGGIGHWMGKRISFESYKWTIAVCYVIHGAAYVLFALSKPFWLALVFIALSRAAVAV
                     SSILNFSQLLRHVSGEFRGRVFSTLESMTWSTMMLSMMGAGIASESMTVRAIGVWSGV
                     LSSSTAVFWAWAHLSGRLPEPPEAGTDPDEIEVHGEPTV"
     gene            complement(46105..47016)
                     /locus_tag="VLE22_20540"
     CDS             complement(46105..47016)
                     /locus_tag="VLE22_20540"
                     /inference="COORDINATES: protein motif:HMM:NF015132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSB16852.1"
                     /translation="MSEPDPHRGAQPSALRLYSLIVLMVLFWAMNFLIGKVALREIPP
                     LLLGGMRVSLAGALILPVYLWMRRRDPVRMNWDRDELPRFLFLGLMGVAVNQLSFVIG
                     LNNTSVAHSSILMSQTPLFVLLLAGIVGQERITGRRVAGMLTAIGGVTVLSVAPAKTS
                     GASLFGDGWILMAALSFAFFTVFGKQITARHGSVTVNMFAYVGGAVMLSPLTLWYAWD
                     FSFAQVSVKAWLSFVYMAVFPSLVCYLIFYYALTYIPASRVSAFSYLQPLLATSAAAL
                     IFGEPVTAGLVTGGALVLTGVFIAERA"
     gene            complement(47013..48128)
                     /gene="menC"
                     /locus_tag="VLE22_20545"
     CDS             complement(47013..48128)
                     /gene="menC"
                     /locus_tag="VLE22_20545"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HSB16853.1"
                     /translation="MEIRVERVILRLVRMPLVHFFETSFGRTTERKIIFVEAIAGGVS
                     GWGEVTAGENPFYNEEWTESAWMILRDYAIPRIQGRTLQSAVEAAPLTGHIRGHRMAC
                     AGLEVALWDLEARLAGEPLWKRIGGGAHKEISCGVSIGIQNSVEQLLGKIETELTAGY
                     QRIKMKIKPGWDVDVVRAVRERWPGIKLMADANSAYTLADADRLKRLDEFYLMMIEQP
                     LAHDDIIDHAELQRRLDTPICLDECIRTAHHAEQAIRLGACRIINIKLGRVGGFAEAR
                     RVHDVAQAAGVPVWCGGMLESGVGRAHNIALSTLPNFVLPGDVSASKRYWSRDVIVPP
                     VEVTPRGTIEVREGAGFGYKLDLDFIRSITIREEAAG"
     gene            complement(48113..48871)
                     /locus_tag="VLE22_20550"
     CDS             complement(48113..48871)
                     /locus_tag="VLE22_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16854.1"
                     /translation="MGGSETTNVIEVRPLRTHAEFGDAVRLQQQIWSFSDIELLPVRL
                     FVVATKIGGQALGAFEGGRMIAFCLAIPGLKSNGLSYLHSHMLGVLPDYTNRGIGRML
                     KLEQRNDAMLRGIELIEWTFDPLELKNAYFNIERLGAVVRRFVLNQYGTTTSALHSGM
                     PTDRCVAEWWISSLYVEALLSGQRPPIPEIEGQIVVPAAIQTIRREDPKQAREVQKSV
                     SEQFLRGFERSLAVVGFERTEKEGIYLLGQWKLE"
     gene            complement(48844..49650)
                     /gene="mazG"
                     /locus_tag="VLE22_20555"
     CDS             complement(48844..49650)
                     /gene="mazG"
                     /locus_tag="VLE22_20555"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HSB16855.1"
                     /translation="MANARSSAAGEKFRRLVEIMARLRAPDGCPWDREQTFDSIKPYT
                     LEETYEVLDAIDSRDWKALADELGDLMLQAVFLARIAEEEGTFSIEDSLDAIVEKLVR
                     RHPHVFADGDAKTAGEVKQRWDEIKRQENREKPASRPGLLDNVPRSMPALVEAQQLTS
                     RASTVGFDWENPDQVLAKLDEELKELAESRRRGLPDEIEHELGDLLFAVVNLARLLKV
                     DAEQALRGTNAKFRGRFGYLERKLNARGRPLSEATLEEMDQLWEEAKRQT"
     gene            49728..50054
                     /locus_tag="VLE22_20560"
     CDS             49728..50054
                     /locus_tag="VLE22_20560"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HSB16856.1"
                     /translation="MEEKFVSLVLFLRFQVELALGFYGAPDGKTAPEPQFEIARHFLD
                     LLGSLQEKTKGNLTLEEDRALNNNLTELRFRYVQAFEESKKKPQAEEAKPAEPQPAGE
                     QEQQSS"
     gene            50082..50858
                     /locus_tag="VLE22_20565"
     CDS             50082..50858
                     /locus_tag="VLE22_20565"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB16857.1"
                     /translation="MTITVLGSGTSVGVPTLGCHCAVCRSEDSRDKRLRPSVALRYSG
                     RVVLIDTTPDFRYQALRAGIERIDAIIYTHSHADHILGLDDVRPFNFRQRGSIPIYGS
                     AQTLAAIRTCFGYAFDGREKESSIPKLETRTLDGTPFELFGVEFTPIEVLHGKQRIYG
                     FRFGAAAYLTDHSEIPEESFEKLQDLDVLFLDALRYRPHPTHSTIERSLGYVERLAPA
                     RTFFTHICHDLAHEQAEQRLPPNVRLAYDGLEITVQEPAR"
     gene            50855..51820
                     /locus_tag="VLE22_20570"
     CDS             50855..51820
                     /locus_tag="VLE22_20570"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HSB16858.1"
                     /translation="MKQAPRVFRNLEAADTFGPCALTIGNFDGAHAGHRRIFRRVTEL
                     ARENGWKASAMTFDPHPTKVVAPHRAPKLLTTPEQRCTLMAQEGIDQVLILPFGPEIA
                     CLTPEEFARRILVERLGARAVLVGDNFRFGHGQAGDTATLTKLGQTLGFVTEVIPAVA
                     LRGRTISSSGIRRLIEAGEVALAARLLERPHFLEGPVVPGRGVGSKKTVPTLNLRVGA
                     EVLPATGVYITRTQALDSGQCWPSLTNVGHRPTFDGGDLSVETFLLEPLEGVVPARIR
                     VELLRRLRDERKFPDAESLKAQILRDASRARAFFRRLGRWVKGAK"
     gene            51856..52860
                     /locus_tag="VLE22_20575"
     CDS             51856..52860
                     /locus_tag="VLE22_20575"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HSB16859.1"
                     /translation="MNLSTAKIQHLNALATPKGIIAAAAMDQRGSLQKSIASAKGVDK
                     KAITPAMMSEFKAAVTKVLTPHASAILLDPEFGLDAAGVRDREAGLLLAYEQSGYDNT
                     QPGRLPDLLPHVSVKRIKDWGADAVKILIYYTPFETTEINDVKHAFIERVGAECEAHE
                     IPFFLEFVGYDPAGGDEKGLEYAKVKPEIVKRSMEEFSKPQYSVDVLKVEVPVNAEFV
                     DGSAVYTGQKAYSMAEALEHFREAAGVATKPFIYLSAGVSNAQFVESLRMATEAGVDF
                     SGVLCGRATWKEGIPVYARHGVKALEDWLAKDGVRNITAVNNAIAGATPWSQKLGVAV
                     "
     gene            complement(52951..54507)
                     /locus_tag="VLE22_20580"
     CDS             complement(52951..54507)
                     /locus_tag="VLE22_20580"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSB16860.1"
                     /translation="MTAVDYFVRELQRRGVTSMATLCGHGLDPLFHAARRAGIRLIDT
                     RNEQTAGYVAFFQGRLSRKPGVCCVSSGVAHINALTGVGSAWFDSTPMLLVSGAGAHR
                     TAGMGHFQDFDQTGLARTVTKYARVIDCPERTLQILDEALNMAVAAPPGPVHLTFPMD
                     IQDAEVDELRLIRTMSPMAAEPPLEDPEPLARALAASSCPLLVAGGGVQSAGGGEALV
                     RFAERFSIPTVVPIWDKGSIDHPAETFMGVIGAATGGPSLLPDADCVIMAGAICDYRV
                     GFLQAGAVAANAAVHHRRAGWDRLSVACDKVGVPPFASWLEAARGRREAFRAGIRRKG
                     DEQAKAGMHAVHIIDALKQVLTGDTVFLIDGGSIGQWVHQLLCDRYPAHWVTCGRSGV
                     IGWGIAGAMAARLCFPERPVILLTGDGAFTFNVAEIECAVRQSLHFVAVIADDQGWGI
                     TRTGHMEQFGESIASSLGPIEMDKLAESLGAKGVKVSRPEEIAPAIRSALAEPAVTVI
                     QVPIVGGNPA"
     gene            complement(54589..55338)
                     /gene="tpiA"
                     /locus_tag="VLE22_20585"
     CDS             complement(54589..55338)
                     /gene="tpiA"
                     /locus_tag="VLE22_20585"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HSB16861.1"
                     /translation="MRKPIIAGNWKMYKTAAETVAFFEKFNPLVAQSTHCEIVICPTF
                     VNLPAAVGAAKGTRIGIGGQNLYFVKEGAFTGEISGPMLAAVGAQWVIIGHSERRQYF
                     GETDASVLKKTVASLDADLNPIVCVGERLEEREAGKTADVLTAQFSGGIAGLDAAQFA
                     KIVIAYEPVWAIGTGKTATPEIASDAHRVIREQAAKQFGKSAADAVRILYGGSVKPDN
                     IKGLMAQPEIDGGLVGGASLDPVSFANIVNF"
     gene            complement(55335..56501)
                     /locus_tag="VLE22_20590"
     CDS             complement(55335..56501)
                     /locus_tag="VLE22_20590"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HSB16862.1"
                     /translation="MGFRRIVLIVLDSVGIGEMPDAAVYGDAGSDTLGNIARMRPLRI
                     PNLCALGLANIRPLAGREPADAPSGCYGRCAAASPGKDTTTGHWELAGVHLNRPFPLF
                     RDGFPRVIIDEFERRIGRRALGNIPASGTEIIKQLGEEHMRTGSPIVYTSADSVFQIA
                     AHEDVVPLWELYKICETARDILHGPYEVGRVIARPFVGSPGSFVRTANRKDYAVPPPK
                     GMLLDRLAERDVPVYSVGKIFEVFLGRGIGDYEKTTSNADGMAKTLAALDEAPEGLVF
                     VNLVDFDMLYGHRNDVEGYAAALEAVDEWIPTLLGRLQEGDLVIFTADHGCDPTTPST
                     DHSREYVPLLAYGPAAKRGVNLGVRATMSDIGQTVAENFGTKVEKGTSFLGAIV"
     gene            complement(56506..57756)
                     /locus_tag="VLE22_20595"
     CDS             complement(56506..57756)
                     /locus_tag="VLE22_20595"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB16863.1"
                     /translation="MRAVVFIALLLAAVCAAQNDPTDAEDSHLRSLLEEAGSGPFEFI
                     RAIEKHLEKFPDSPRRAEMERSLVKAAIETKDDDRILRYGERVIAQGDDDPKTLERVI
                     RVLLSQDDKDRATRALRYAREYEDAVRLIEKEKPTRRSDQVLWRGEVDRGFGRALVFQ
                     ARALSILGRHEEAASAAAAGYERDPSAETAREAALTLMRTGKEMEAVERFAEAFAISD
                     PRNTDAARADDRKHMGELYRKLKGSETGLGDLVLQAYDRTAALLEKRREDVRRLDPNA
                     GLEDPMQFTLSSLNGEDLALSSLRGKVVVFDFWATWCGPCRVQHPQYEAVKKKFGERG
                     DLVFLSVNTDEDPNMVAGFLERNGWDKSVYFEDGLSRLLRVSSIPTTIIANRRGEIVS
                     RIVGFDPGRFTEILSERIREALGE"
     gene            complement(57756..58379)
                     /locus_tag="VLE22_20600"
     CDS             complement(57756..58379)
                     /locus_tag="VLE22_20600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16864.1"
                     /translation="MRFATLLLLTAALAAQDAPRIFYSKSFPQSSLPFVSITVEKSGK
                     TEYKEAPDDPHPIDLQLNEAETTSIFELADKLDRFNRKLESGLKVANMGIKTFRYEAG
                     GQNHEVKFNYTQDADAQLLADWFERITETVQHSINLEKTARFDRLGVDRALINLQVSV
                     ERNRLAGAEVLLPMLDRVAKGSAYMNRARERAASIAEYIRSGKQKSE"
     gene            complement(58376..58870)
                     /gene="ruvC"
                     /locus_tag="VLE22_20605"
     CDS             complement(58376..58870)
                     /gene="ruvC"
                     /locus_tag="VLE22_20605"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HSB16865.1"
                     /translation="MRILGIDCGTERTGYGVIESDGRAHRVVVSGVIRTRPADPMEHR
                     LLAVSQGLVDVIRVHEPEVVAIEEVFHAANVKTALKLAQVRGVALLAVAEASLPLGEY
                     SPLEIKTSVVGYGRAEKQQVQMMVTSLLGLDQVPSEDASDALAVAICHATHSAHEARL
                     EASR"
     gene            complement(58886..59632)
                     /locus_tag="VLE22_20610"
     CDS             complement(58886..59632)
                     /locus_tag="VLE22_20610"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSB16866.1"
                     /translation="MSGHSKWATIKHKKAAADAKRGKVFTRVIKEITIAARGGGDPDA
                     NPRLRTAIIAAKAVSMPADNIKRAIMRGTGELEGGQLDEVMFEGYGPGGAAVLVQVVT
                     DNRNRTVSEIRHIFSKQGGNLGEVGSVAWMFERKSQILIDQSKAAEEQLMDLVLDAGA
                     DDLRNDGVNWEILSPPDTHDKVLEAIHKAGIPTVEAQIAMVPKNTIKLEGKNAGGMLR
                     LAEALEEHDDVQNIYSNFDIDEKEMEALAG"
     gene            complement(59705..60280)
                     /locus_tag="VLE22_20615"
     CDS             complement(59705..60280)
                     /locus_tag="VLE22_20615"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HSB16867.1"
                     /translation="MAVIILGISWLVFGFGAFFLYIRAEWERRSRPVTADGPELRSST
                     SLYGLVLETLAYVFVWLFRRFGAGHEPLLLVIIGVLLAPASVAFFMWALLHLGREFRL
                     QAVVGPGHQLVATGPYAAVRHPIYLSMLGLLVSNAMVVSRWEAALAGLAIFIAGTEIR
                     VRTEDGLLARRFGEAFDRYRGRVPAYLPYFR"
     gene            complement(60304..63135)
                     /locus_tag="VLE22_20620"
     CDS             complement(60304..63135)
                     /locus_tag="VLE22_20620"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSB16868.1"
                     /translation="MKPEPSPQPDSDLADALRFSESQLPVIFESIGIGVAVVDTTGRF
                     LHCNPAFAALVGYSLDELRRMRFTDLTHPEDIAAELEIFRRATTVDGAESGATLTKRY
                     IRKDGRIVWARLVAQVARDAVGRAQYGFGIVIDITAQKEAEDQLRKLSVAVEQSPASI
                     VITNTAADIEYVNPKFTELTGYTPSEVLGRNPRLLQSGETPPAVHRELWNTLRAGREW
                     RGEFRNKKKNGELFWENASISPIKTASGAVTHYLAVKEDITERKRMEEEISRQLRMNE
                     SIAEMSAHLLSLASHEEISSSILRHAQLHTDSPHGFVGYVNPRDRTFVAPTLSGDVWA
                     DCRIEDRSVIFGRFGGLWGWVLKNKLPVLTNDVPSDARSTGVPAGHLPIRRFLAVPCV
                     LGDEAVGEIAVANSGRDYTDDDLDLLKRLAAVYTMAIERQRTEEERTRIQEQLRQSQK
                     LEAVGQLAGGVAHDFNNLLTVINGFTELAMSRLNPLDPLSQELEEIRKAGDRAASLTQ
                     QLLAFSRRQLLKPKLIDLNAAVADVEKMLRRLIGEHIELTTVLDPNLGKIKADPGQIQ
                     QVIVNLAVNARDAMPGGGRLTVATKNVELHSHHLLEDPLVQPGPYVMVSVADTGTGMS
                     KKILEHIFEPFFTTKEQGKGTGLGLPTVYGIVKQSGGSIRVDSQPGLGATFSVYLPRA
                     EDAADEHQGVKLGGESPGGSETVLLVEDEQDVRELAARILESCGYKVLVASNGDEALT
                     AAAQYPGVIDLMVTDLVMPGMDGRELAQRITAVRPQTKVLLISGYTDAPESRRLPFPP
                     DSAFLQKPFTRSDLASKVRELLDRAQNSATVLVVDDEAEIRRLLRRILEAAGYDVFEA
                     GNGRAAMEQLAHRSADLVITDIVMPECEGIEMIGSLHKERPEVKIIAVSGAFGGQFLH
                     VAQAVGAHAVLQKPLRAERVLAVVRQLLG"
     gene            complement(63272..64129)
                     /locus_tag="VLE22_20625"
     CDS             complement(63272..64129)
                     /locus_tag="VLE22_20625"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HSB16869.1"
                     /translation="METSPAAGLLTKRFVAGQTLDEGLGVCRKLNSEGILVTLDHLGE
                     NVTSEEAALAAAAAYLESMQRIAEHQLDASISIKLTQFGLDLSEEFCRENVERLVSAA
                     AGIGGFVEIDMESHEYTDRTLRIVAAMHERFRRVRGVIQAYLYRSASDVRMLNEKGVP
                     VRLCKGAYKEPSSVAYAGKREVDTSYGHLMKELLDHGSYPALATHDETLVNEALRFVR
                     ERGIAPDRFEFQMLYGIRRDLQRRIVASGYRLRLYVPYGGAWYPYFMRRLAERPANVL
                     FLARNLFRR"
     gene            complement(64199..65383)
                     /locus_tag="VLE22_20630"
     CDS             complement(64199..65383)
                     /locus_tag="VLE22_20630"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HSB16870.1"
                     /translation="MGLVVGATIGGLVLALWMRGKLAWVEAAKNVAEARLTAADQAAD
                     KIGEKFQALADAALRSSQGAFLDTARSSLETVRAEMSGDLAQRQTAIEGVIQPLTGAL
                     SRLEGQVKELELERQQMFGSLREQIQSLGKETVTLANALRNPQVRGRWGEITLRRVAE
                     LAGMVEYCDFVEQETHDAEDGRIRPDMIVKLPGGRSLVVDAKVPLAAYLDAVSSDDEE
                     GRRDALNRHGQQVRKHIDQLSSKQYWSQFQPAPEMVVLFIPGDHFFSAALEYNPTLIE
                     DAVDRKVLLATPTTLISVLKGISYGWRERRLAENAEQIRQVAVEVSDRIQIIHGHYSD
                     TGRHLERAIEAYNRAIASWESRLLPSLRRVRELGAVNGTEPLAPASVEVMPREAVVSE
                     PS"
     gene            complement(65505..66695)
                     /locus_tag="VLE22_20635"
     CDS             complement(65505..66695)
                     /locus_tag="VLE22_20635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16871.1"
                     /translation="MPAQENSKRIAVITTIYRYLSHAQHMADRFLVGYPLNGRWHRSR
                     MKVASLYVDQKPDGDLSETRAREYGFRVYPTIAEALRCGGDKLAVDAVLVIGEHGDYP
                     RNEKGQVLYPRYEFFRECVEVFEKDGRTVPVYNDKHLSWSFEKAKWMVEASRRLKFPM
                     LAGSSLPVTWRLPDVELPLGCEIEGALMTGVGGSDPMDYHALEAMQCMIERRKGGETG
                     VKAVQLVEGDAVWEAGERGRWSRELLTAALSRSDTPQGLTLTDGRPQDLVGSGVLPQL
                     VKNPAAYFIEHRDGLRTTLLMLNGAIKDYNFAARVKGIGTISTQFFLPPVPNVTYSAC
                     LVGKIEEMFETGEAPYPVERTLLVSGVLESCLTSRVQGHARLETPHLDVRYRPPARSQ
                     HAQA"
     gene            complement(66699..67961)
                     /locus_tag="VLE22_20640"
     CDS             complement(66699..67961)
                     /locus_tag="VLE22_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16872.1"
                     /translation="MRISRRDLLKAAGSAPLAARAQRRPVKPKIAALTTIYHTHSHAQ
                     HIVDRFLDGYGWNSAHHRPPMDLVSLFVDQVGSDDLSRERASRHPSMRVCASIAEALT
                     LGGSSLTVDGVLLIGEHGRYPRTEKGQTRYPRYEFFQEIVKVFRASGRSVPVFNDKHL
                     SWNWDYAKEMYDTARSMGFPLMAGSSLPVTWRTPPLEMPLGARVREAMCVAYGGPDSY
                     DFHALETLQCMVERRAGGETGIKWLQAFRGEEFWHAHKEEVWSPDLFEACLCRSHTLT
                     PAREGYNHIYPSMEDLARLVKNPIAYRYEHEDGLVCTMLLMNGLVEDFNFAARIDGHA
                     DPFSTQMYLPMPPRRTSTATFFTPLVNNIERMFLSGSPPYPVERTLLTTGLTAAGVES
                     LYQKQARIKTPHLAIRYAPPRESTFWRT"
     gene            complement(68043..69260)
                     /locus_tag="VLE22_20645"
     CDS             complement(68043..69260)
                     /locus_tag="VLE22_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16873.1"
                     /translation="MTRKAFCSWVLLASLALGLMGGYCWQRLERRELALPPPSGTPIW
                     SFAAGLSAVVSADKEFVVLAGESSDELLAYLRYDYLRSRRESSRMQVLLTTRETGGRP
                     VYRFYLYPWNRDAITAASSASGLVASGYIAGFELAWVARSVLADWQQQTRTFEAAYSL
                     PVRRHLEGIGRGHLLNLMARFIHFKSATDARIRRGDEPAPEPLSPDEARGMAANIITV
                     AEFYELPLEFFLGIGAMENNYMDIRGDLRHVVWKRRAQKGDIILRRRRGRVLVLNYAT
                     GPWQITRETLRYVHRLFLKDTRDYGRLPLSLRPPRKLNLDEIDSGLLTTYAGLLFRHL
                     IDRFNGDVARAVGAYNGGIRNPNMGYEEGVRLVADYARNILEQAAALNNQSVSEMSFF
                     APSRRSPEPKPKS"
     gene            complement(69304..70053)
                     /locus_tag="VLE22_20650"
     CDS             complement(69304..70053)
                     /locus_tag="VLE22_20650"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HSB16874.1"
                     /translation="MNLVWRETYRVRFYEAEPGGRASAPAICRYLQEAADIHCRAYGL
                     SLDDLRAAGRLWVLTHLSLRLTGLPRVGDEVAVETWGTRRLGGVRAYRDFVLLDAAGR
                     TFGEASSLWLLLDAATRRPVRLPESILRFRHPERAGADRVDAMGAIPPEQPSVEERNR
                     VRWHDLDANGHANNVCYVDWAIETVPLALRRDGRLSALDVRFVSEAFLDDEVISAAEE
                     FPTADAPSYRHALHTGDGRLLAAARTEWTRI"
     gene            complement(70085..71014)
                     /locus_tag="VLE22_20655"
     CDS             complement(70085..71014)
                     /locus_tag="VLE22_20655"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB16875.1"
                     /translation="MPHSITRRDALLAAVALPVTVSAKAGKTVTIGMATTEFRDYTNA
                     RMAQELKGQGIRVIQLFLTQTDSNYWKYNSRSDLAGLTAARATEIAGIYRSAGISIHS
                     IGVYTNLIHPEEAERKANLAYFDAMMTVGGHMGVRKFISEAGHWRGTEPEPPVPHHWQ
                     EEVWRQMLVTGKELARIADSHDATVLLEPYFQGFLASARRTRLFLEEVGSPRIRALLD
                     PANLLEVDSIEAMFDQLDPWIDCFHAKDRKLHVTRGVPAGEGDLDYRKFVTLATERKP
                     GAPLFLEYVGSKNYTQALAQLRGVIRGAGLAEA"
     gene            complement(71015..72352)
                     /locus_tag="VLE22_20660"
     CDS             complement(71015..72352)
                     /locus_tag="VLE22_20660"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB16876.1"
                     /translation="MTGKSTRREFLGASAAAGIAIPRTYGAGAVAAPGPNDTINLALI
                     GCGARGRTLIIPRFLGLPGTRFVAVCDVNSKHLADGRQRAGGDRVAAYNDYRKLLEDK
                     TIDAVIIATNQHWHVLPMIAACQAGKDVYLEKPLGNSIGEGAVAIEAAKKYGRIVQTG
                     TQQRSQQHYREAVELIQSGKLGEISEVRVWDCENYWPGSGSPADCDPPPELDWDFYVG
                     PSPWRAYNPNVYYNYGYDWFKVSGAGHQVAWGVHHFDVVLWAMGVKSPKTAFASGGNF
                     AFEDNHEYPNTFTGTLEFGPGPVSKRGFVLNYTMRTGCRRETRAHGKCFMGSEASMVL
                     DRAGYSIVPEVKKGQRAVPTTGLVNPEQTVPPKDDHDYHFEVFLNNIRARRQPEATVE
                     TCHYATAVGHLLNISWETGRSIRWDGEKNRVIGDPQADALVLRPYRAPWKLEI"
     gene            72549..74159
                     /locus_tag="VLE22_20665"
     CDS             72549..74159
                     /locus_tag="VLE22_20665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HSB16877.1"
                     /translation="MSSVFGPYAQEDEFGSRSINPMELYHNQVTRAQGVFSLRMFHRS
                     WGIMMIQQNISNPSTVLDFPKRDDFIREIRNHRYDIIGISSIIVNVGKVAEMCRLARE
                     HSPGSTIIVGGHVSAIPGIEKMVDADYIVRGEGIAWMRSYLGQDAGAPIVHPEIVSGF
                     DMRVMGFKIPNKMSDTAATIIPSVGCPMGCNFCTTSAFFGGKGKSVDFFETGDELFEV
                     MSRMEERLNVRSFFVMDENFLLNRPRAMRLLERMKEAGKSWAFYVFSSANAIRKYSME
                     ELVQLGISWVWMGLESVKSSYAKLKDTDTVALTAELRKHGIKLLGSTIVGLEHHTPEN
                     IREEIERAVAHDTDFHQFMLYTPVPGTPLYHQMKQEGRMLEDVDLADIHGQWKFNFKH
                     AAISRDESKHFLDLAFWRDFERNGPSLYRICRTTFEGWRRYKNHQDQRVRERFQWEVR
                     TLRRAYSGMLWAMERQLRDSNEAVSRRVRELRHELEREFSGFSKLATGVLGPMFLWMS
                     KREQKRLEAGQTYEPQVFIERRNWEAAV"
     gene            74164..75066
                     /locus_tag="VLE22_20670"
     CDS             74164..75066
                     /locus_tag="VLE22_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16878.1"
                     /translation="MLRRILAPAFALLTLGQGFAIAQQAVDIDIWRTAGPFSAGLARP
                     VKVVDSDGRDASPNVSRDGSRIVFSSTRSGTWEVWTCRSDGSGVFQVTRLGDTPVGST
                     RWSPDGKRIAFDGQKPGHYDLFVVNADGSGLRQLTTESSTDVKPSWSKDGRWIYHTSN
                     RTGEYQVWKILSAGGEAVQVTRRGGYTPFETMDGKFVYYAKGLNEHSLWRVPASGGEE
                     SFVLDNVVSNRWVMLKNGIAILNMDAQPLPVIEFYDFATGRRRALDVLPKEGNIYGGG
                     TAIAAPPGGAWLLLCAIEPAKRPE"
     gene            75107..76573
                     /locus_tag="VLE22_20675"
     CDS             75107..76573
                     /locus_tag="VLE22_20675"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HSB16879.1"
                     /translation="MGRFLLTASLLLCASGAYSGELQDDLKARRARLMEKLGPKSMLI
                     LHSASVKNYSWDVDHEYRQDSNFYYLTGVEQEESVLVMMPGNSNRREVLFIREKDPKR
                     EHWQGRSLSQAEAAEQSGIETVYWLGEFERFVEMILSGEPYRPEKAPRLDRSPDFDTF
                     LHALAGGEAELAVVLDQTPTIKGPVNPALAFAHDLRDRFLGFRVVDATRHVHALRQVK
                     SPYERKVLEQSAAISGEAQRAGMRAARPGAYEYEVKAAIEQVYRGRGAFGWSYPPITG
                     SGPNANILHYAKADRRMDAGELMLVDAAANFQYYTVDITRTYPVNGRFTEAQKDIYSI
                     VLRAQEEAAKFARPGVLLRDVHHKTVDVIKDGLQRLGLLTDISGDQYSVWYTHGSTHY
                     IGIDVHDVGDYQEPLAPGHAFVIEPGVYIREDAFESLDKTPENEEFTKKVKPAFEKYK
                     NIGIRIEDSFLMTENGLVNLSAKAPRTIEEVEGFKKGR"
     gene            76617..78956
                     /locus_tag="VLE22_20680"
     CDS             76617..78956
                     /locus_tag="VLE22_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16880.1"
                     /translation="MPKKLVRRKPEKQKTKKPRVKRRVPPPETPPDIEAPPVPYQWIT
                     ATLRTSTGLFCPITSSPLLRRRAMEFAYSVRNRAHWAEVESAREQLKQRGSQVLKEFG
                     LSDTDLRQIAEAGIVEVKIPYPAESIGWEVRILPWEYLLSAATKPFRGDRALHVVRYL
                     ERTTAAGPAAKTPASLLFVETAPGPLSEDYSFEFERKLVEANLELDADHLINPTLDAL
                     KNRIGHPTPDVIHLTGFDTHQGVDLLDWHLKQPKDGWKDGMFFRGPDGLPQIVEAIPM
                     SEAFEAVKDHPPALVAFNCYNSASRLAALAVGAGAGAAIGFQDDIDDFLAELFYTNFY
                     RAWKLSGWNILQAFKWARNSLRGQSSKLRGTGVILWSATPLLGLEEAPDELEKRFRDD
                     HRRAVQVPPPAQGGVREIVTVEVEPLDKLNYSLLHNDRPLFDTFQIRKYPPGRLSGVS
                     VEVMLHAGPESLRYQSSFDLEDPIKDLKDLVKVPLTSELARSIRESVQTCVFVNVSCG
                     GQDVFRDTYPVTLLPIDEWQDDDLNRVWLPSFVMPRDPVVARIIDAGQRYLMSLADDA
                     GAGFDGYQSVDPKSDDPSEGVDLQVRALWSALLNEYRLGYINPPPTFTDSAQRLRTPV
                     DIVESRHGTCIDLALLLASCLEYVDIYPVVFLLEGHAFPGYWRSDSAHEKFWERFYVK
                     TFEQTEEPPDERMVPNAAGAEPWMLGAEGYASVVELVRSGDLVPLESVWLTQQRGFWE
                     AVESGGENLRSKRDFQFMVDVLRARRQKVTPLPVLGIRK"
     gene            78953..80293
                     /locus_tag="VLE22_20685"
     CDS             78953..80293
                     /locus_tag="VLE22_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16881.1"
                     /translation="MTPPFPSADERQEMNRLLVNNLDQAVDDLDIFTPDREFDAEISI
                     SQLRRGRREALADPVGMMTLVEVDGVLLWQEGAAVSSPALRRRRGRRAEGPGGEIVQQ
                     FKFEKLEPSKVGAFLERMDLKLTPERGLRKWDGGKLAAIDKPADKGRILLIVHGTFSN
                     TDAVVGELRATAGGQAFLDRAVAHYSQVLTFDHPTISVSPVLNALDLARLFDSSSADV
                     DVVCHSRGGLVTRWWMEAFDRKQRGRRAVLVGCPLNGTSLAAPPRLRAGLSLLTNVGR
                     VLAPAADAASAVIPFLSVAAGLIRVLSSLSSLAAASPMVDAAVAMIPGLAAMSRIGNN
                     PELSRLNQDPASPPAEYFAVRSNFEPSSPGWAFWRYFVSPKERLVDVAVDTLVFDGQN
                     DLVVDTASMTELPPASAPPATRVLDFKTNDTVYHTNYFRQPQTLKFIADSLQIP"
     gene            80602..83061
                     /locus_tag="VLE22_20690"
     CDS             80602..83061
                     /locus_tag="VLE22_20690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB16882.1"
                     /translation="MISTMTREIRYSLRTLIKSRGFTAAALLALALGIGANSAVFSLV
                     NAVLLRPLPYEDPAGLVDISELDAERQGLPVDYLDFVEWRAQADAFKRMAFQATFTQT
                     LTGAGLAENLPVGFVTADFFGTLGVRPILGRDFVASDDRKTAPPVAILSYRFWQNRFG
                     GDRGVLGRTLRLGQRNFTIVGVLPSSFRYHRPGQVYVAFGPCLSQWGMDQRGNHNNAF
                     VVARLKPGVSIERARAQMDTIARRLEAQFPLNRGIGAAVRPLRELFAGGVRSQMLILL
                     GAVAFVLLIACVNVANLMLVRSAARRKEIAVRAALGARRLRLIGQLLTESILLSMAAA
                     LLGLLLAEAAFDVLAKLLPWGFEAADVSIDWAVLAFTLSVGCSTGILFGIVPALQATD
                     FSLSEALKESGRFGDAASARRKLRSALVIAQVAISMVLLAGAGLLARSFWRLVQVSPG
                     FEVQHVLTMDVSWPYWEILGVAGAVDFHKRLCERVEALPGVRAVGGIWPLPLGTGGAA
                     IPIYRAGKPVPAQGQCPVVAYHRATPGLFSALGIPLLRGRIFTAADGTPPRDQSPAQV
                     EAAWRASTLAAVISESMARRFWPGEDPVGRRFRFGPPEFNGPWVQVLGVVGDIKGAGL
                     DQPAEAEFYLSAYLDPNDLTLVVRSGGDPAALAAAVRTAIAELDRNVPVDNVRTMDKV
                     LADGVSRRRTNMLLIGAFAMLALVLAAVGLYGVMAYLVAQRRHEIGVRIALGASGADV
                     ASAVVKEAGLLALAGVGLGTLGALALTRLLANLLFGLAATDPATFGAAAAVLLAVAVA
                     ASWAPARRAARVDPIVALRCQ"
     gene            complement(83115..84122)
                     /locus_tag="VLE22_20695"
     CDS             complement(83115..84122)
                     /locus_tag="VLE22_20695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB16883.1"
                     /translation="MEPKTTRRVFFQAALGTAALAEQVIAQTSSAAATGLPTRVLGRT
                     GVRVSFLGLGGAHVGRVKEEAEAIRIIHTALDEGMTFFDNAWEYNNGRSEDLMGKALA
                     VSGRRKQAFLMTKVCSREYKPAMEQLEESLRRLQTDHLDLLQFHECNYYNDPDWVFEK
                     GGIRALMEARKQGKVRFIGFTGHKAPAIHLKMLAQKFDWDTAQMPNNVMDSAFVSFRD
                     EVMPVCLKKNVGIIGMKGCGGDGRVITEGKLSVEDCYRYCLSQPVAVQVVGLTSMEQL
                     REAIRIGRAFKPVTAAETRTLLSRVGEAVGDGRYELFKSSKAYDSSYHRTQHGFETRG
                     V"
     gene            complement(84144..84989)
                     /locus_tag="VLE22_20700"
     CDS             complement(84144..84989)
                     /locus_tag="VLE22_20700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB16884.1"
                     /translation="MMNRRQFLQTGLLATSALSPALGAKRKATDVVRLGPDKVKLSRL
                     AIGTGTKGGSVQRLLGVQGVADMLQYGYDEGIFFWDTADGYKTHPHVKEALKRVKREK
                     VTILTKTRARTADQMRADLDRFRQELGTDYFDIMLLHAVTNPLWTEERAGAMEVLSEA
                     RQKGIVRTHGVSCHSIEALRTAAKTPWVHVDLARINPASILMDADPNTVVSVLRQMKA
                     AGKGVIGMKILGEGGLRNRVDEALRYAMSLDCVDCFTIGPANRDELKELLTKIPAASE
                     MRKAA"
     gene            85116..85520
                     /locus_tag="VLE22_20705"
     CDS             85116..85520
                     /locus_tag="VLE22_20705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16885.1"
                     /translation="MNRRTFLTLPLAAVAAGAAPSRPNVLILKPVAREDLHFTIESRD
                     GFHFAVRRGEWKLVRHEPRPSGGAVNQLYRLDEDPQETKDVAAAHPALVKELAASIEK
                     WRKLYPAEGTRLENRPPAGWKAPSQWAEAARR"
     gene            85715..86266
                     /locus_tag="VLE22_20710"
     CDS             85715..86266
                     /locus_tag="VLE22_20710"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB16886.1"
                     /translation="MARTQAAPALDLGQALVESYAVNDRMNQVVLEYLDPGAWRAKLP
                     GSRGRTIAAIFSHLHNVRRKWLRLSAPRIKLPAPLDGARCSQKQARVALAESAARCCE
                     MLAGALAGPERRVETFRRDGWARPWPAGAAMVAYMISHEAHHRGQVCMLAHQLGFPLP
                     VKGAYRIWVWEKLWKECGFTHPR"
     gene            complement(86563..87945)
                     /locus_tag="VLE22_20715"
     CDS             complement(86563..87945)
                     /locus_tag="VLE22_20715"
                     /inference="COORDINATES: protein motif:HMM:NF004992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HSB16887.1"
                     /translation="MTKQFEAIIIGTGQAGPFLAHRLAAAGMKVAIVERKLFGGTCVN
                     TGCIPTKTMVASAYAAHTARRTWDFGVVIDGGVSVDMRLVKARKDAISAKSRIGTEES
                     LRQLENCTVYQGHARFESPRSVSVGPERIAADRIFINVGGRAAVPPMPGLEHVEYLTN
                     SSMMDIDFLPPRLIVVGGSYVGLEFGQMFRRFGSDIAVVEMGPRLVQREDEDVSVAIQ
                     EILEGEGIRLRLNAKCIGFSKRGEEIIARLDCAEGPSEISGTHLLLAVGRRPNTDDLG
                     LDKAGVELDQRGYIVVDDQLRTSVPGIWAMGDCNGRGAFTHTSFNDSEIVAANLLDND
                     SRRITDRIPAYALYIDPPLGRAGLTEAEVRKTGRRALVGNRPMTRVSRAIEKGETQGF
                     MKIVVDADTREILGAAILGVGGDEAIHSILDVMYAKAPYTVIQRAVHIHPTVSELIPT
                     MLGELQPLTG"
     gene            complement(87942..88409)
                     /locus_tag="VLE22_20720"
     CDS             complement(87942..88409)
                     /locus_tag="VLE22_20720"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HSB16888.1"
                     /translation="MPTYMLAFLIGVVAGLRSMTPPTAVSWAARLGWLHLENTPLAFL
                     GFAATPYILTLFAIAELINDKLPKTPSRKTLAPFTARIVTGAFSGAALGAPSGALLGG
                     LLAGALGGVAGTLAGYEFRRRLVKATGGKDLPIALLEDAIAICAAFWTVSRAL"
     gene            complement(88505..>88884)
                     /locus_tag="VLE22_20725"
     CDS             complement(88505..>88884)
                     /locus_tag="VLE22_20725"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB16889.1"
                     /translation="DAQVTPLMSKDLPECPGKEGQMITVVYPPGASDPIHRHNAHAFV
                     YVLEGTVVMQLKGGKEITLRPGQTFYEGPNDIHTVGRNASSTEPAKFIVFLVKDKGAP
                     VLIPVKPTAAGVSASPKLNSGLK"
BASE COUNT        16699 a        27054 c        28962 g        16169 t
ORIGIN      
        1 aaaggccgct ccgacccgac tgcccaaccg gaggtgcggc tcatccggat tcacgaaggc
       61 aagaccacca cgctggcctc aggactcggc acgttcctgg cgggcgcgat cgcaacggcc
      121 gcgaaacccg acccaagcgg ccgccggctc gcgttggtcc ctattactgc caataacaaa
      181 ctggccgtca tcgatctcgc ctccggtaag cctgcgggga cactccctac cgaacttgtg
      241 ccgttcggcg ccgtgctctc gcgtgatggt gccgtcgcct acgtgagcaa ttggggagga
      301 cgcccgccga aacccggaga tctgaccgcc ccgctaggtc tcagaccgga cgccgaccgc
      361 gccgtcgtgg acgagcgcgg tattgcctcg acgggcaccg tcacacgcat cgacttgaag
      421 gcgatgaagg caacccatac gatccccgct gaactccacc cgacggccct cgcctgggac
      481 gagcaacgga accggctcta cgtagccaac gccaatacgg actcgatctc cgtcattgac
      541 acgcaaacgc aaaccgttgt cgccgccttt ccgcttgtcc cgttcgcggt gaaggcattc
      601 ggcatcgcgc cgacggcgct ggtgctctcc ccggacggcg cgcagcttta tgtggcctgc
      661 ggcggcatca acgctgtaat gttactcgag gcgggcaccg gacgcatcga gggcatgatc
      721 ccgacagcgt ggtatccgaa tgggctggcg ctcagctcgg acgggaaact tctcgcggtg
      781 acgtcgctac tcggcgccgg ctcggggtgg cgcgacgagc cgcgcaagcg tttcgtgcac
      841 tcgtaccgtg gggcgctaaa cgtcatcgag atccccgaca aggcgcaact ggcgagctat
      901 acgaccgtgg tgtcggaaaa caaccggttg ccgctcgcgg gctccgcctc ggaacggccc
      961 gcacagccgg ccgtggacgc gaagccggtc gccattccgg cgcgcgccgg cgaactatcg
     1021 ctgatcgaac acgtggtgtt catcatcaag gagaaccgca cgtacgacca ggtgttcggc
     1081 gcgctgccac agggtaatgg cgacccgtcg ctggtaatgt tcggccgcga cgtgacgccg
     1141 aatcaccaca agctggccga gcagttcgtc gtaatcgaca acttctacgc gaccggcggg
     1201 aacagcgccg acgggcacca gtggctgacg caggccaacg aggtcgccta ctgcctgtgg
     1261 ccgggctacg ctgggcgcag ctatccgttt gacggctcgg acccgctcgc gtactcgcac
     1321 agcggcttct tgtgggacgc ggcgctagcg cgcgggagaa ccgtccgcgt gtacggcgag
     1381 tatgcgggac gcctgccggt tccgtcctcc gagcggcagc cgctactgga gcgctggcgc
     1441 aacggtgagg atttcacgca gaggtggaac atcaccgcgc ccatcgacaa gctgaatacg
     1501 atcctcgcgc ggaactatcc gacgtacaca acgcacattc cagacgtggt gcgggcgcag
     1561 atcttcctgg cagaccttcg caagttggag caagagggca ggatgccgaa cctcgtgatg
     1621 atccagcttc cctcgaatca caccaacggc gcgcagccgg ggatttcgtc ggccaaggcg
     1681 atggtggcgg acaacgacct ggcgatgggg aagatcgtcg aggcactctc tcacagccag
     1741 ttctggccga agatggccgt cttcatcgtg gaagacgatg cgcagaacgg cgtcgatcac
     1801 gtggacggac atcgcacggt agcgctggtg gcgagcccct acgccaagcg tggcgcggtc
     1861 gactcgacgt tctactcgac gcagagcatg gtgaagacga tcgagctgat tctcggactg
     1921 ccgacgcttt cgctgttcga cctgatcgcg cacgacatgc ggaacagctt cacggacacg
     1981 cccgacctga cgccgtataa agcggaggag cccacgcagt ctctgttcga gcaaaacccg
     2041 ccggtcgaag ggctcaaggg acccgcacgg gaggcggcgg ttgcatcaat gaagatgcgc
     2101 ttcgacgtac cggatgcggc gccgtcggac cggctgaacc gcatcctatg gcaccaggta
     2161 cgcgggtgga acacacccta tccaggcacg cgcacggccg tcttcgcgcc actctcgctc
     2221 gacctcgacg atgacgagcg ggaggaggcg gtgggccggg agtaagatgg tttcccgcaa
     2281 aggtgcgaag gccgcaaaga tctgattcgt gtggactagc tcaaaaccaa cgatacgtgg
     2341 tgccagaaac ggggacaaac gcgcctgacc gtcacaccgg acggttgagg tgagcctctt
     2401 cgacggattc gcgctcattc gcgttcattt gcgaccttga tcagtgggtg atatcgaggg
     2461 tgcggacggc ggggtcgggc aggtcttcga aaaggaagcg gaaatcgggc ccgagttgcc
     2521 gcaggcggag ccagcgcagc aagacggtca atgcagcgat ggctgacagg accagtgcaa
     2581 aactggcggg acggtcgagc aatcggtact cgactttcgc catgtagtga gcgtacatcc
     2641 aatacgccgc caggtagacg ggccagaacc ctttcacgtt cgccttgccg ggcaggtaag
     2701 tgcaggtaaa cgggatctta tcgaagttca gcagcaacaa ctcaaccagc agcgccgaaa
     2761 ggaccagccc gtatgcgagg tgcgcgccgg ctacacgcca tccccaaagc aacacgtggg
     2821 ccggaagcaa tccgagcaac acgggataaa tggcgagcgc aagcatcgct ctccggacgc
     2881 cggcgcggca gcgtgccgtc tcggagggct ccgccgcctg aaacagccag ttggcattca
     2941 gctccgccgg caccgtaaag gcgaaacgca ttcccgaaac gacgaagaaa gatagcacga
     3001 gctgcactga gagtacggcc ggaaccggcc tgccgccagc agagtaacgc agaattacag
     3061 ccgcgctgcc gaggaccagg gcgagcccga cgcccacata ggctgccatc aaaagccggt
     3121 ggacgcggct ccgcagcaac gtcaaccgga tgaactgaaa gcatcctcgc tcgagggggt
     3181 gggtgaggac gaaatggttc agcaacactg ccgccaggcg cgccatgaac cccggaccgg
     3241 agcgcccggg ttcgagtgac tccagcagtc tcttcatgtg acgccggtaa ctgatccagt
     3301 aggccaacgc ggccaccgcc gcgcagacgg tgatagccgc aacggcaacc ttcgccagcg
     3361 gcgagaacac cggactgccg cggcccatca actcctggta cagtccgagg aaccagaagg
     3421 gcgggaacgc gtacatccat gggtcgttcc cgtgcacccg tgctgtgaca gagaagagca
     3481 gcattggcat aagaagaaag aggagaacca gtgcgagtac cgtggcgagc tgaatgtaaa
     3541 ccgacaggcg ccgaaaccac ttcggactca gtatgttcaa cattatgcct tgcaggccga
     3601 cgaagaacag gaacacgaag gagcacgcgg cgaaggtcgc gatgatatgt ccggctacga
     3661 accgcccgta atcaaagctg gacttggtaa cagaacccac gattgccgga aacatgacgg
     3721 ccggacccag gttcaccgct gcccacaaga tgcccagcag caaaagcacg gatgagagtt
     3781 tcgcggcgaa cagcaggcgc gttgtgatcg gaagcggcac taacacctga tagtcgcgcc
     3841 ggtcagggaa cagagcatcc cattcaatca cggagagcaa gccagctagg atcatggaga
     3901 agccaatgaa caggatcttg tcaggccaag tgattatctc gcgctccggg tagtcgaccg
     3961 ccgcgtactt gagcagcatg agcaggcagt agaaacagcc gggtgcggcc accaagccaa
     4021 ggatctgcgt gaatgcggcg tgcatcccgg tttccggcga gctgatgagg tcgttatcaa
     4081 gaaaaccggc gaagaagtgc tggacaagac ggccgaagcg gctctcccga atcgacatgg
     4141 cgcgctcctc tcaggagatt ctcatcgctt ccgcgatgtc gctggcaata ccctcggtgc
     4201 tttcctggag cacgagttgc gcgaacacgc tctccagcga cggcgcctgc aacaggtcgc
     4261 gcagctcaag gacgtcgccg tccgccgcga tgcgcccgcg cttgaggatg acgacccgcg
     4321 agcagacctt ttcaaccgtt tcgagaacgt gggagctgta gaggatgatc ttgccgcggc
     4381 cggccagcgc acgaaccaga tcgcgaaaaa cgagcgctgt cgtcacgtcg agaccggaca
     4441 gcggctcgtc gaagacaagc agctcggggt cgtgcagcag ggcgccgcag atgagcacct
     4501 tttgccgcat tcccttggag tatgaggcca tcagggagtg gcggtcggac tggagaccaa
     4561 acagacgcag cagtgcatcg gctttttcgc cgagggcctt ctcgggcagg cagcgaaggc
     4621 gaccgaccag cagcagatac tcccgcccgg tgagataagg gtagatgtgc ggctcttcgg
     4681 gaacgtagcc gaccgagcgt ttgtagccgg gcaggtcgtc tttgatgtcc cgtccgtgga
     4741 agtagacgtg gccgcgggtc ggatcgagaa gaccagtgat catcttcacg gtagtgcttt
     4801 tccccgaacc gttggggcca agatagccga ggacctcgcc aggatggatc gtgaagctga
     4861 catcgtccac cgccgggatg gccgagtaat gcttcgtaag tccatgaacc tcaagcatgc
     4921 cgagttagac accgggtcaa cagccggtgt tcccgggcgg gcggacatag aatgatgctg
     4981 gatggcgccg gcataccggc agggccaatg catgctacaa atcgtcgtga tggaagtcgt
     5041 cgcccagtcg atcgacgtcg aggtggtccg ccgacgggcg gcgcgtggcc agtcagtggc
     5101 tgatgttgag ggtgcggacg gcggggtcgg gcagatcttc gaacaggaag cggaattcgg
     5161 gtccgagttg ccgttgccgg agtcagcgca ggatggccgt cccggcggcg atgctcagca
     5221 ggaacagtgc aaaaccggcg gggcgttcga gcagtcggta ctcgatgttc gccatgaagt
     5281 gagcctagat ccagtacatc cccaggtgga gcggccaaaa ccctttcacg ttcgccttgc
     5341 cgggcaggta ggagcacgtg atcgggatct tttcgaagtt ccacagcaac aactcaatca
     5401 gcagcaccga aaggaccaac ccgtatgcga ggtgggcgcc ggcgaactgc cagccccaca
     5461 gcaaggcgtg ggccggtagc aatgcgagca gcaccagaca gaccgcgagc gccagcattg
     5521 ctctccgtac gccggagcgg cagcgtgcgg tttcggacgg ctccgccgcc tgaaagagcc
     5581 agttggcgtg caactccgcc ggcaccgtaa aggcgaaacg cattcccgaa acgacgaaga
     5641 aggacagcac gagctggact gagagcaggg ctggcgccgg tttgccgcca gaagccgaca
     5701 ggagaatcac agccgcgccg ccgaggacga gtgcgacacc gacgcccaac ggcgcctaca
     5761 acaggtcgcg cagctctaag gacgtcccca gtccgccgcg acgtgccctc gcttgaagat
     5821 gacgacgagg acctcgccgg gatagatcgt gaagctgaca tcgtccaccg ccgggatggc
     5881 cgagtaatga ttcgtaagtc catggacgtc cagcatgcgg agttagacac caggtcatga
     5941 gccggtgttc ccggtaaacg tgttgtacaa agagcacaga gaaggtgggg ccctgcgcgt
     6001 ccggtagctc atacgccgcg gcgggctccc cagaggtcga cgtggaggcg ggggctgaag
     6061 cgaaagcctt cccgcttgca gatctcggca atccagaggg aacgctcgtg gagggtggcg
     6121 gggtcggttc cttcgggcat caggactacc ttcagacggg acgcaccaag gtccgtgacc
     6181 atggcaagaa tctcgttgag atcttcaggc tgctggacga caaacttgag ctggtagtcg
     6241 tagtccgaca tcaggcgggc caggacctgg ggctggaagc gaaggcgatc gtgttgagcg
     6301 gcccatcgac caccgtcacg cttgcgaggg gtggagttgg acgtcttcgg gctgatgctc
     6361 atgaggtcgc aagcaacggg ggcgaagacg gtgccggcgg tctcaatggt gatgtgcagc
     6421 ccggcggtgc gcaggccctc ggtgagcggg acgatctccg gcgcgatcat cggttcgccg
     6481 ccggtgacaa caacgtaacg cgcgggatag cgccgcacgt ccgccaggat ttccccgacc
     6541 tccatctccg tgccttccgg ctgccacgat gtgtagggcg tgtcgcacca aacgcagcgg
     6601 aggttgcagc cggaggtacg aacgaaaacg cttggcgtac ccacgagggc gccctcgccc
     6661 tgagtggagt agaaaacctc cgagattctc acgagcggta ttccagcggg tccttgagtc
     6721 ccgcctgctg gaagcccttg agccggagca ggcacgaatc acaggcgccg caagggcgac
     6781 cccgttcgtc agggtcatag caactgacgg tgaggcggaa gtccacgccg agcgcggcgg
     6841 cgaaccgcac gatttccgcc ttgctcagac ggataagggg tgtgtggatg cgcacccgag
     6901 tgttgccctc gacgccggcc ttggtcgcga gcacggccat ccgctcgaag gcctcgatgt
     6961 actcagcacg acaatcgggg tagccggagt aatcgatggc gtttacgccg atgaagatgt
     7021 cggacgcgcc gagcacctcg gcccaggcca gcgcgaacga gaggaagatc gtgttgcggg
     7081 ccgggacgta agttatggga atccctagtc ctatcacttc ggcggggcgc gccttgggca
     7141 cttcgatgtc cgctgtgagg gcggagcctc cgatggcgcg cagatcaaaa ctcacgacaa
     7201 gatgccgggc cgcgccgaga tcagcggcca cgcgcgccgc ggcctcaagt tccgctcggt
     7261 gtcgctggcc gtagtcgaag gagagtgcat agcactcgaa gccttccttg cgtgcgaagg
     7321 cgaggcatgc cgaggagtca aggccgccac tcaagagaca gaccgctttg ggccgttcgc
     7381 tcatttcttc agtttcaggg tcaggaagaa cccgacgacc gggaagacca gcagactggc
     7441 gagtacgttg tgcatcgaga agaggtcgga ggcccagccg gtgagcggga tgaagatgag
     7501 gcccgcggtg ccccaggcga atcccatcat cagagcagag acggtacccg cctgcgtcgg
     7561 agcaagttct tgcgccataa caacattcac gggaatcgta aacaaaagga tgaatccgcc
     7621 gaggatcagg ccgagcaagg atgcccaccc gctcccgaag aaaaacaagg ccaggaaggg
     7681 caatgagccg gccatcgaga gcagaatgac ggttcgcccg ccgaaacgat cggcgagatg
     7741 gccgccgaga aagcccccaa gcgcgccggc agcgaggtaa cccgagagtg catagttggc
     7801 gcggctgacc ggaaagccct tttcgaggtg gaggtaaagc ggcaggaact ggccgaaggt
     7861 gatctggatg atggagcgga tgaagaccag cacgtaaagg atcgtgaggg gcttccggac
     7921 ggcgcgcaga accttccagt cgaagcggtt gcgcggatgt gtcatggagg agggcgccgg
     7981 gggcagcaga gggaacagca ggagcgtgag gaccactcca ggtacagcgg cccaatagct
     8041 tcgcgagagg ccgagggcgc cggtgattcc ggagaaaaag gtcgggccga gcgcgtagcc
     8101 gagagctccc gcgctgataa agatggccat tgcgatgccg cggcggtcgg tagacccgaa
     8161 ggcgacgcga gcggaggctt gcgggtgaaa cgaggcgatg cccgtgccac caaggacaac
     8221 catcggcagc atccagacat aggaaggtgc gagcccaagc gacgagatga agatgccggc
     8281 ggtagcggga gcgagaacgg tgaacatccg cgtgtggtac cggtcggaga ggaagccata
     8341 gaccggctgc atcactgacg aaaagaagac gaggagcccg ccgaggatac cggcctgagt
     8401 gaggttcgta ccaaagcggt ccacaagcaa gggctggagg gcaccgagcg cgcctgagta
     8461 aaggtcgaca aagaaatgtc ccagggacat gaggacgagt gtcgccactc ccgcgcgagc
     8521 agcgcgctcc gcggcagaat gacttgacac ttcggctacg ttggcttcgg aactcattcc
     8581 gggggactgt cttcgattat accgagagga gattcgggct aggcggtgcg cggtcgtgca
     8641 aaggggcgca ccgccggttc cagtccttcc gaggccctac acgaggtcga gcaggaaggc
     8701 gtaaatgaac gcaatttcct tgtagcgctt gtcgcggccg gaggcaccgc cgtggccggc
     8761 ctccatttca gtcttcagga ggagccggtt cttatccgtc ttcatggccc ggagtttggc
     8821 cacccacttg gctggctccc agtactgcac ctgcgaatcg tgcaggccgg tggtcacgag
     8881 caggttaggg tagtgtttcg cggccacgtt gtcgtagggc gagtacgaga gcatgtagtc
     8941 gtagtacggc ttctcgtttg ggttgcccca ctcgtcgtat tcggcagtgg taagcgggat
     9001 gctgtcgtcg agcatggtag tcacgacgtc gacaaagggg acctgagcga cgacgccctt
     9061 gaacagatcc ggacgcatat tgaagatcgc ccccatcagc aggccgccgg cgctgcctcc
     9121 ctcggcgaac aggcgctccg gactggtgta cttctctttg acgaggtagt ccgcgcagtc
     9181 gatgaagtcg gtgaaggtgt tctttttctt gagcagcttg ccgtcttcgt accagcttcg
     9241 ccccatctcc tggccgccgc ggacgtgcgc gatggcataa gcgaagcccc ggtccagcag
     9301 gctgatgcgc atcgcgctga agttggggtc cgtgctgatg ccgtaagagc cgtacccgta
     9361 caacagcagc ggagccttgc cgtctttccg gaagcccttg cggtagacga gagagatggg
     9421 tacttgcgtg ccgtcgcggg cggtggcgaa gagacgctca gactggtagt tggtgggatc
     9481 gaagccaccg agaacctcgt cgcgcttcac gagcttcttc tcgcgcgtaa ccatgttgta
     9541 gtcgtagacc gagttcggcg tggtcagtga actgtagtcg taacgcacga ggggcgtgtc
     9601 aagctcatag ttctgactcg tggcggcgac gtagactggc tcgccgaagt cgatgtagtg
     9661 ctcgccctgc cccgaccacg gcatcacgcg gagttggatg agacccgcct tacgctcact
     9721 cacgacgagg tggtccttga aaatctccat atcctcgaag aaaacgtcgg cgcggtgcgg
     9781 taggacttcc ttccagttcg ccacggctgt ccgagcgact ggggtcttca taagccggaa
     9841 gttcttcgcc tgaaggttgg tgcgaatgta gaactgatca ccgaagtgat cgatctggta
     9901 ctcgtgatcc cgctggcgag ggaggaagac gcggaactca cccgcaggct ttgcggcgtc
     9961 gaggaaacgg tactctgcac tgagggtctg gcgggaggcg atgaggatgt acttcttcga
    10021 cttcgttttc aggacgtagg tgctgaaggt ttcgtctttc tcctcgaaga ccagcgtgtc
    10081 gccggcggga tcggccccga gtacgtgccg gaagatttgg taggaacgta gggtttccgg
    10141 gtgctgacgc gagtagaaca gcgtcttgtt gtcttcagcc cagacgacgt tgccggtgat
    10201 gttgcgaatc acatctctgt actcctcgcc ggtctcgagg ttgcgaaatc ggacatcgaa
    10261 aaaccgccgg ccgacaagat cgaccgcgta ggccatgatc gctccactgg tcgtgacggc
    10321 gggcggcggg accgagcaga agccgtggcc ggcggcgatc tgattgacgt cgatcagcac
    10381 ctgctcagga gcgtcgggcg aacccttcct gcggcagtag atggggtagt ccctgccctg
    10441 ctcgacgcgc gagtaatagt agtagccgtc cttgcgatag ggagccgact cgtcggtctg
    10501 cttgatgcgc gttttgaatt cgtcgaacag cttcgcctgg aggtcggccg tatgggccat
    10561 gatcgcttcg gtgtactgat tctcggcctt gaggtaatcg atcacctcgg gcttgtcacg
    10621 ttccttgagc cagaaatagt tgtcagtgcg gacatgcccg tgtttctcga gtttcgtcgg
    10681 catgatcttt gctacaggtg gtttcggccc ctgccggcaa ctggagctgg ccaggaaaac
    10741 agtgatcagc atagcggcgt ggaaagtacg cataacagtt ggggtaacca ttcgataata
    10801 cgccggaacg ggtgggcggt agtcaggacg ccctgaacag cttgctgcgg atcagggaga
    10861 gaagttcgat ggcaccgtcg acttcgacgg tgatgtcgag ccagtagagt tttagcggtg
    10921 acagcagtgc gagggcgcct tcactgatat tcatggcgtc cttctcggtg gtgagcagcg
    10981 cttcaatgcc ggcagtacgg gcttgggtag tgaggcggcg caaatcgacg ggcttgtatg
    11041 tgtggtgatc gccgaaagtc cagcgggcga gcggttcaat ttcaagggcg gtaagcgtgc
    11101 tccagtacga ggaagggttg gccagaccgc agaaggctcc gagcctcgtg tggggcaggc
    11161 cgcgcggatc gaactgtccg acggttcctg cttcaaccca actttttgga acaacgcgcg
    11221 agaagaagac cggtgccgcg cggttgtatt ctcgcagccg gtcgacgatg gcgtcgtaac
    11281 cgcggcccgg ctcggtacgg gtcataacga aggcggcggc gcgcgacagg gcgcccatcg
    11341 gttcgcgaag gcgtccgagg gggaagagat gctggttagg cccaaaaggg gccagggcat
    11401 caatcaacac gatgtcgagg tcgcgggcga ggcgccagtg ctggaagccg tcgtcgagca
    11461 gcatcacatc cgggcggaag cgttcttcga cgaggcggcc gacagccgcg cgaccggcac
    11521 cgatgccaac gggaccgacg ccggagcgga gaaagatctg ggcttcgtca ccggtccgcg
    11581 cgggggaaac ctggtcgccg ggaccgagga tagtatgctt ctccgggacg cggcggcggt
    11641 agccccgggt aagaatcgca ggagaaaggc cggagtcctt gagcctctca gccagccaga
    11701 gcacgaaggg cgtctttccg ctgccgccca tagtgatgct gcccacgctg attactggcg
    11761 tcgagagccg gcggcggcgg gccgtatcga tggccctctt ggcgaggcca ccgagccacc
    11821 agaggccgga gagcagccac aagatttgat agagggggaa ggctgggcgg tacacaggca
    11881 gggcgcgtga tcgcagttgc tcgatctccc gcacggcacg gtcagtcgcg ccccggcgag
    11941 cttcggcgag ctttcgggca cgctcgccga ggagggagcg gcggtcgggg tcggagagca
    12001 gggtgtccac cgccgcggcg agttccaagg ggctgtcgat tcggacgcag gcttcggcct
    12061 cgatgaactc ggccgcgatc tccgggaagt tttccatgtg cgggccgatc accacggggc
    12121 gggcgaagaa ggcgggctcg aggatattgt gaccgccgcg gtggggcagc gttccgccca
    12181 tgaagacgac atcggcgtgg gcgaagagag cgctcaattc gccgatggag tcgagcaaca
    12241 ggacgcaggg aagcgcgggg agaggcggct gatccgcgag cgcggtgcgg cgcacgaagg
    12301 ggacgccggc ggcagtgagt ttctcggccg cggagtcgaa acgctcgggc cgtcggggca
    12361 cgtggatcag cagcaggcgt ggacggctgg gcgccagctc gcagaaagca gcgatgacgg
    12421 aatcatcttc atcggggtcg ccgggttcgg ctggcggcat ggtgctggcg gcgatccaaa
    12481 tggagccagg tttgaggcga tcgagggcct tcctgacctc cggggcggct ccgcccgagg
    12541 ggggctcgaa gtcatacttc aaattcccac cgaggacagc cggcgcgcca agctcgaggt
    12601 aacggcggag gctgacctca gtctggacga gaatgcggtc ggcctgccgg agaactgggc
    12661 ggaagaacca gcggaggcga tggtagcgcg gcgcggcgcg atccgagatg cgcccgttga
    12721 cgacgatcag tgtgcagccg gcgcgtttcg cctcgcggta gagattcggc cagatttcgg
    12781 tttcgagcac gacgacgaca gcggggcgga tggtgcgcag aacgcggcgg acggcgaaac
    12841 agtagtcgat gggcgcatag aagactccgc agacgacgga ggcgagtttc tcgtcggcaa
    12901 gggcgcggcc tgcgagagta gtagtggaga caaacagcgg cgccgtgggg aacgagcgtc
    12961 ggaggccttg aagcaggccg ggagcggaga gcacctcacc gaccgagacg gcgtggatcc
    13021 agatggctcc cggagccggg cgtttgcagg agtgcgcaat gaagcctaaa cgctcgccaa
    13081 agcgtcgaaa gtaacggcgg tcccgcaggc cgcgtccgag aaaatacaat acgatgaaag
    13141 gaaacccgag ccactgcgcg gcgcggtaga gagagtaaat gagcttgtca tccaagtggc
    13201 tgatcttcct catgagtata gtcgcagcat tcggggcgga caaggataag ttcgatccgg
    13261 ggccggtgac gtcgctcgcg gcgaggcaaa cgatcgggaa ggtgacgatc ggcgcgcggg
    13321 agttcgacac tgaagaactg gcgcgtacgg cgttcggcaa gttgaatcca aacaagtacg
    13381 gcgtactacc cgtgctggtg gtgattcaga acgacggcga caagacgctg tcgctcgacc
    13441 ggattcattt ccagtacctt gtgcctgggc acgacgagat cgacgcgacg ccggcgaccg
    13501 acgtgaagta catcggggga gcgtcgaagc cgaggacggg accgtcgccg ctgccaattc
    13561 cgcgtggcaa aaagaaaagc ccgctgacgg cgtgggagat cgaggggcgg gcgctggcgg
    13621 cgcggatgtt gccgcccggg cagtcggcga gtgggttcgt ttacttccag gcggcgtcgc
    13681 tccagggggc gaagctgtac gtcacgggca tccgcgacgc gggcagcggg caggaattgt
    13741 tctacttcga aatcccgttc gacggcggga agtagcgaca agtggcagga ctcccctcct
    13801 gtgcattgtt cgatcgcagg tcaggacatc cgctactttt tctttggtac agctttcctc
    13861 ggggcggctt tttttgcggc cgggggcggc acgggggcgg cgccgacgcg ctcgatgacg
    13921 acgctggtga tgcggaccgg ggtgcgcggt ttgtcattgg caccgcgcgg gacattggcg
    13981 atcttcgtca ccacatcctg gccctgaatc acctggccaa aaatcgtgtg ggcgccgttc
    14041 aggtgtggcg tggggacttc ggtgatgaag aactggcaac tgccggtctt cggtccggcg
    14101 ttggccatgg cgaggcggcc gggctggtcg aacttcaacg aagggtggaa ctcgtcgggg
    14161 atgacgaagc cgacgtcgcc cgcaccgtca ccctgcggat cgccgccctg gatcatgaag
    14221 ccgggaataa cgcggtggaa tgtgagcccg ttgtataggg gcttccgcac catctgtccg
    14281 gatttcgggt ctttccaagc cttgcggcct tgtgccaagg acacgaagtt cctgacggtg
    14341 atcggtgatt ccttctcgta gagcttcacg gtgatatcgc cctgcgtggt cttaaacgtg
    14401 gcgtagaggc cgtcttcgcg cgcggcctgc gccggtggcg tgggcgcctg ggccgtctga
    14461 gcggcaaggt tcaacgtgag cagcaacaac aaggacagag caatccagcg agaagtctcc
    14521 atgtccgagg attataccaa caccgtgaac gtggtctcac tccggacgac cggtcggtag
    14581 agggtgtcgc gctccgcggg ttcgcgacct gcttcgcgga tgaggcgcag caactcgccg
    14641 cggcgcagcg actgcgttgt ggtggcgccg gcgtcgtggt agatcttttc ctcgaccacg
    14701 gtgccgtcga cgtcgtccga gccgaaacgc tgggcgatct gcgcgatacg cggcgtcatc
    14761 atcacccagt aagccttgat gtgcgggata ttgtcgagca tcaggcggga aacggcgata
    14821 ttccgaatgt cgtcgaatcc ggtagttttc ggaatgtgac gcagcggcgt gttgtcaggg
    14881 tggaacgcca gcgggatgaa ggtgacaaag ccgccagttt cgtcctgaag ctcgcgaagc
    14941 ttcatgaggt gatcgacacg gtcctcttcg gtctcgatgt ggccatacag catcgtgcag
    15001 ttggactgaa ggccgatgcg gtgggcctgg cgtgcgacgt cgagccactg cgagccgtcg
    15061 atcttgtggt cacagatgat gcggcgaaca cgttcggaga agatctcggc gccgccaccg
    15121 gggagcgaat cgacgccgga gtccttcagg cggcgtagcg tctctcccgc ggtgatcttc
    15181 gtgcgatgcg cgaggtaggc gatctccacc atcgtgagcg ccttgagatg cacctgcggg
    15241 aagcgttgct tcagcccgcg gaaagcttcg cagtaccagt cgagcgtcag ctcggggtgc
    15301 agaccgccga caatgtgaaa ctccgtgatc gcttcactcc agccgcgccc agccgtctcc
    15361 cacacctgtt cgagcgacat ggtgtacgcc ctctcgtcct tcactctctt tccgaaggca
    15421 cagaggcggc agcttgcgac gcaaacgtcg gtgggattga tgtggcggtt gacgttgaag
    15481 taggtgacgt tgccgtgacg ccgctcgcgg acgaggttgg ccatgtaacc gagagcaagg
    15541 aggtcggggc tgcggtagag cgttacgccg tcctcgaagc tcagacgctc gccggccgcg
    15601 accttgtcga ggatagggcg gaggcgcgcg tcatcgatga tcggctgcat ggctcaggtc
    15661 ccctggcgca aaaggaagat atcagcggcc cagaataaga agaataacac gctgacgtag
    15721 ccgtttacgg tgaagaaggc cgcgttgacg cgcgagaggt cgccggggcg gactaggctc
    15781 tgttcgtaag ctagcaacag ggcaacggcg ccgagcccgg cgagactcag agcgcccagg
    15841 ctgaaggcgt agacgagcgc agcgaggcaa ccgatcatcg cgaggtgaaa gaggcgcgag
    15901 atccacaggg ctccggcgat gccgaagcgg cgcgggagac tgaacagccc ttcgcggccg
    15961 tcgaactcga agtcctgaca agcgtaaatg atgtcgaaac cggcagtcca gagggtgaca
    16021 gcagcggtga gccagagaat gcgcggatcg agggaaccgc ggatggcgat ccaggcggcg
    16081 gccggcgcga tgccgagcga gaagcccaga acgaggtgcg agagcgatgt aaagcgcttg
    16141 gtgaacgagt agaagaacac cacggcgagg gcagccgggg ccaggtagaa gcagaggctg
    16201 ttcagggctc gggcggccac gatgaagacg gcggaggcgg ccgccgtgaa gccccacgcg
    16261 aagcggcggg agaggacgcc agccggcagg tgacgcgcgg cagtccgggg attgcgggcg
    16321 tccatcgggg cgtcgaggat gcggttgaac gccatagcgg cggagcgcgc ggcgaccatg
    16381 gcgactacga tccagaagac cttccggccg agatgcgggg tggcgaagcc gtcctcgcgg
    16441 acggcaagca gcgcgccggt caacgcaaac ggcagggcga agaccgagtg ctcgaagcgg
    16501 atcatatcga gcgtgagcca gagacggttc caaaagcgac gcaacattgg ggcacgaccc
    16561 gcgtggggcg agtctcacta ttctaccgga cgcgacatcc ggtcagcgcc gcgagaaaga
    16621 ttcgatacca ttgaagacgg aatgcgacgg acgatcaaga cggcgatcgc gtgtgccgcg
    16681 ctggcactgg ctgggtgcgg gagcaagacg gcgcttgacg aatactatac gcaggtggtg
    16741 acgctgccga acgggaagga gatacgggcg gaagtgatgg cggaccggac ggacatgatg
    16801 cgaggcatga tgttccgcga ctcgctggcg ccggaccgcg ggatgctctt cattcatgac
    16861 cgacccgacc ggcacacgta ctggatgtac caggtgagga tcccgctcga catcgtctgg
    16921 ctggacggca accgtaggat tgtcgagatc tcggcgaata ccccgccgtg caagtcacag
    16981 gccagcaagt gcccacaata cgggggcaag gagatgtctc aatacgtgct tgaactggcg
    17041 ggcggtgtgg cggcacagca cgggctccgg ttaggagagc agctaacctt ctgatattct
    17101 cgaccaagga catgagcggc gagcaactta ggaaattatt ggaacaggta cgcgcaggcg
    17161 gcgttggcat cgacgccgcg gtggagcgga tgcgccacat gccgttcgag gacctcggct
    17221 tcgccaaggt ggaccatcac cgcgcgctgc gacacgggat gccggaagtg attttcgccc
    17281 agggcaagac acccgagcag gtggtggcga tcgcggggcg gctgctcaag tcggcaagaa
    17341 acatcctgat cacgcgagtt gatgagcgca cggcggcgct ggtgaagcgc aagatccgga
    17401 aggtggagta ttttccgcta tcgagggcga tccgcgtgtg gcaggaccgc acgattcgag
    17461 gcaagggcaa gattgcggtg gtgtgtgcgg ggacgagcga catcccggtg gctgaagagg
    17521 cgcaggtgac cgctgaggtg atggggaacg aggtcatcgc gattcatgac atcggggtcg
    17581 cggggattca ccgcctgatg agcaaccggg agcggctaac ggaggcgaag gtggtgatcg
    17641 ttgccgcggg catggaggga gcgctgccaa gcgtggtcgg gggcctggtg tcctgtccgg
    17701 tgattgcggt gccgacgagc atcggctacg gcgccagctt caacgggctg gcggcgctgc
    17761 tggcgatgct gaacagttgc gcgagcaacg tgacggtggt gaacatcgac aacgggtttg
    17821 gcgcggggta cgtggcgagt ttgatcaacc gcgtgtagtt tcctgcgtgt cgctggccgt
    17881 caggcagtct tcgcggtaag ggcctggcgg cccatctcct cggcaaagac gtagaaaccg
    17941 aagagtggtt tcgaggctcc acggccccag cggctcacct tccactgcac gcgaagatag
    18001 cgaacttcgg gcctatcgtt gaggtcgagt aggacctggt aggtcccgca gtagaatttc
    18061 tgggggtatg agagcagggg ttttgtgccc caatccgcgt tatcggcgga accccagacg
    18121 gagatgtcga ggctctcctg ttcgatgatg cgcgtgattc cgagggtcag cagcaacagc
    18181 cttccttgta agtcaccgag ttcgctgtcg ggacctcgtc cgtcctcgcg aacgactgtt
    18241 tccggaacaa gaaagttcgg tagcaccgta gcctcgcgga gaacccgctt cttcagcgtc
    18301 tcatattcgg cggccaaaag gaaagccggg ttgttcgctc aactgtggtg ttgccttttg
    18361 cgtgccattc aaacagagcg accgcaggag cggcagcccg ccccgctcac agccgggcga
    18421 gacggcgggc ggcttcgtcg agggtttcag gcttcttgca gaagcagaag cggaccattc
    18481 gggcgccgtc acggggatcc tggaagaaac tggacccggg gacggcggcc acaccggcgt
    18541 gctcgatgag gtggcgcacg aaggccacgt cgtccggaaa gccgaagccg gagacatcgg
    18601 tcatcacata gtaggcgcct tttggagtga aacagtggaa gccggcgcca gcaaggatcg
    18661 agagcgtccg gtcgcggcga accgcgtagt cccgggcgag cgtttcgtaa tactcatccg
    18721 gcatcgataa cgcggtgacg cccgcctgct gcaagggcgc ggctgcaccg acggtcagga
    18781 aatcgtggac cttgcggatc gaatccgcca gatccggggc ggccagggcc cagccgacgc
    18841 gccagccggt caccgaatag gtcttgctca tgctgttgac cagaatcgac cggtttcgca
    18901 tgccgggcag ggtcatgatc gggacgtgcc gggcgccatc gtacaggatg tgctcgtaaa
    18961 tctcgtcggt gatggcgagc gcgtcgaact gctggcatag ccgggcgatg aactccagtt
    19021 cctcgcgcgt gaagaccttg ccggtagggt tgttcggcgt gttgaggatg atcgccttgg
    19081 tacgcggtcc gaacgcgcgg cggagctcgt ccggatcgaa ggtccagtcc ggaggacgca
    19141 acttgacgag ccgcgggacc gctccggaga ggcgggtatc cggcccgtaa ttctcgtaga
    19201 acggctcgaa gatgacgact tcgtcgcctg gattggtcac ggcgagcaac gaggcgatca
    19261 tcgattcggt ggcaccacag cagacggtga tctcgcgttc cggatcgacg tcgatcccgt
    19321 accaatggcg gtacttggcg gcgatggctg cacggaacgg ccgagcaccc caggtgatcg
    19381 cgtactggtt gataccggcg gcgatggcct cgcgggcggc ctccttcaac tcgccgggag
    19441 cgccgaaatc cggcatgccc tgcgcgaggt ttaccgcctt atgccggatg gccagacggg
    19501 tcatttcacg gatgactgat tcggtgaact gtgaggcgcg atcgctcagg aaacgcgcgc
    19561 ggtcacttct tgccggcaac ggctcgcttg cggaaagccc catctgtcga gggtacctca
    19621 gtggctttcg gaccgtaaag gcggcggtac acctcggcgt tggcaagcac gacctgcacg
    19681 tacgtacggg tctcggtgaa cgggatcgtc tcgatgaatt cggcgggctc gcggaacggg
    19741 ccccatgtgg tccacacgtc gacacgggac ttgccgccgt tgtacgcggc caaggcggcc
    19801 tcaacgtctc cgcctagttc gtcgagccgc tggcggaagt aataggtccc aagccggatg
    19861 ttgtaatcgg ggcggaacag cagcgcggaa cggaaccggc ccgaacgggt tttacgggca
    19921 aggtaacgtc cggtggacgg cagtacctgc atcaggccgt aggctcgggc acgagagatg
    19981 gccgtgcggt cgaactccga ttcctggcga atcagcccgg cgagcacgaa cggatcgagg
    20041 ttccgcagcc ggccgtactt ctcgagcgac tggcggtatg gcagcgggaa ggctaggcgc
    20101 cagaactcgg ctggcgcgga ttcgagcggc gtatagaggt aggtcggcca tacacctttg
    20161 atgtgccgga tcgccgtctc cgggaccccg cgccgcgagg cctggcgggc gagttccagg
    20221 gccagggacg gcgcgtcgcc atttgcacgg gcctcaaagc gaagctccgc gtcggcgaag
    20281 tcggacagcc cggtggaggc gagcaggcga gcgcgggcga tgcgcagacg ggactgctcg
    20341 cctggcttaa aactgacggg gccgccgcgg accggcaatt gcaggccgtc gaaggcggtg
    20401 ggcggcatgt tggatccggt attccgggcc agtggtccct gggtgagcct gtcctcgcag
    20461 aggaaggcgt agtagcggtt cgggaagtgc tcgaccacct tccgatagag agcgcgcgct
    20521 tcaccgctgc ggccagcctc ctcggccgag cgcccgagga agtagagcgc ggcgctcgct
    20581 ttggggcttt cgggataccg cgagaggtgc tccgccatca ataccggcgc cgccggggtt
    20641 cggcgcatcc actccgccca ggcaaccttc cagtgacaat ttgacgcctc tgccgaagcc
    20701 gggaacgact catagcaggc tcggtacaac ggctcgaagg cggcaacgtc gtttctcagc
    20761 aggtactcgt tggcggccca gaccagggct ttcagccgcc attccgattg cgggtggagg
    20821 cggccaagct cggcgaggtg ctcctgcata gcctgcgtgt cgtcgaggcg gcgggcacaa
    20881 gccgtcatga agtacaggcg ttcggcggca gcctcgggcg acagggatcc aagggacttc
    20941 aggtattggt aagccgtctg ggtccgttgc gccatgtaat ctgcaacgcc gatgcgaacg
    21001 cttgccaggt cccgctccag cccgccgagc cgcgggatga gcccttcgaa ttcgtcgcgg
    21061 gcgcggccat attcgcgcgc ttccagccac ttccccgccc tttcgagcat cgcgtgaggc
    21121 atgggtggcg gataaccggc tcccatcgcc gcccggaggc gttccaacgc cgacgcggcc
    21181 gccgacgcgg ccgaggtagc aggaaactgg aagtagaccc gctggtagta gcccgcggcg
    21241 gcaggcaggt ctcccgctga ttccagagcg gaagcgagcg cgaagtcgcc ggcaggctgg
    21301 gtcagacggg catacccggc acgaagaacg gtgatcgcat cggtgggcgc tccagactcg
    21361 gtgtacgcct tcgcggagag aatcgccgcc cggctggtta acggagatgg cggggtagct
    21421 tcgagcacgg tcctcaactc gcttgcggcc gcggccgggt cgcccgcttc cagatgcgaa
    21481 gctccaaggt gaaacgcaac gtagtcgtcg agctggggca accgcttacg cgcctcgctc
    21541 agcctcttga tcgcctccgg gtagcttccg tcctcgaagt cgatgacacc caaggcaaag
    21601 agcgccatgg cgccctggga gtctttcgga tgcgccgcgg cgaaccgtgc aattgcgtcg
    21661 cgaccggcga cgctggggcg ctcacggcag gcgcggacca acgactccag gttctgcgcc
    21721 ggaagcacca gcggaaagag cagaaggaaa gtgagcgcca gtgtccgcat cggttcacac
    21781 gagaggcccc gggtactccg gtcccaacaa agcaggatca tcgttggcca gcgtgcgaac
    21841 cagttccagg tggaaataat cgaccatagt gggacacgaa acggcaaact gaagccggaa
    21901 cgcctcccgc ccggcgtcga tgtcgcccct cagatcggca taaaggttgg aggacaggcg
    21961 gccggagcga acttcgcggg gcttgtaaag ccgcatttcg gcaacctgga cgcgagcgaa
    22021 gcgctgcgcc cgcaggtgga gatcctggtc ttccggagga agcgccggcc agtccggcag
    22081 tttggagctt ggaccgggcg cctgcccggc aatcatgacg aggccggagg acgccgccgt
    22141 cgcggccgcc cggcgagaat ccagggcgcc tccggcaagg gccgtgagaa gttccagtgc
    22201 ggcggtgtga agccgggttt cgtgtccgtc ggcgtacaac aaaccgaccg ccttgccctg
    22261 gcaaaccacg gggaagaaga acgaacgccc cgcggagtct tcgcccagcg cggacgccag
    22321 cgtttcggaa agctcccccg cggtgcgcat cgcgacaacc gtatcgagtg actccacggc
    22381 gccggcggct gccggagaag cttcaagggg cgtctcggcc acgagcaaaa cgccggccgc
    22441 gtccggatcg agaccgcggg ctccggcgca ccgcaatgct acgccctcga cggcaaacag
    22501 aacggcccga tcacagaatg catcggccgc atcgagcagg acggcgtgca gatcgtcgcc
    22561 ggattcggac tgccgcatcc ggcgggcggc ttggttcaac tgttcggctg tctcgcgccg
    22621 cagcgtttcg cgctggccgg cgattcgcgc ggagatctcc cggccgaatt cctcttcgac
    22681 cagggaagca agctcggcga aacggtcttc gaggacgcgg tcgatctgct ctttccagcc
    22741 tgcggtgagt tgctcctcga cgcgctcgac gtggagctgc caggctgcct tcagctgttc
    22801 acgcgcggtg atgcgttgct cttcgagcgt gctcgtgaga ctgaagtccg tccaaggcag
    22861 cagtcggggt gtttccatgt tcaatagcca gtgtatcgaa tcgccactcg ctcacgactc
    22921 agcgcggcgg ttctagacgg agttcgagag gcgagaggct gccggcgaga cgcagcggat
    22981 gtgctccgcc ggagagctcg acggatatac gcccgtcttc gcgcgccacc gcatgcccgg
    23041 tgagaacgtc cgtgcccact gccacccgca ggcaatccaa ctcgacgctg gggcccttgc
    23101 cgctccacaa ggcgctgtag catccggaga ccaacggcag ctctgtgtcc ggagaaagcg
    23161 cgacggaccg gccctggaac gttccttcag cgtgcatccg ccggagaagt tcgtcaccgg
    23221 accccgacgt tctgagcctc gcgtctccac cgagcgttcc accgcgccat gctatcgagt
    23281 caacggcgac accaaggtca tagactggct cacgcccgcc cacgccaatc gaaagatggc
    23341 cgtcaatacg gccttgtgcg acccgggcgg agacgcgagt gacttcgagg cgctcgccat
    23401 cccaaaacca gcgtgcgcga acggactcga agtcgagtcc ctggactgca aagtttccaa
    23461 tctccagcgt gccttcggcc ttccgtgctc ggagccactc cggcagcggt gaccgcccga
    23521 acgccagcgc ccgggagagt aggccggcct gccgctggag ggtgggcatg aacagccact
    23581 cgagttcgcc cgcgcgcagc cgctccacgc ggcaacggat gcggtgcggg taccgggccc
    23641 cgggctggtg gcgatagtcg ccttggattg ccgtctcacc gagggcgcct tttacgtccg
    23701 ttaccgatag acggccgcca tcgagtttca cggatgcggc ttcgatggcc agcggcgcgg
    23761 ccaggccgtt gacctcgacg ttggtcccgg tcacgcgaat ggcgcctgac cactcgcccg
    23821 gcgtaccggg gttctgacgg tatcgcagcg ctccgctcca cgcgcctccg cggaagtggt
    23881 ctaggaacgg gacgcggccg atccccgccg gaaggctttg gccacggtcc ggaccaccaa
    23941 ggcttagctg ctgggtttgg acctgaaggt cgacacgctc ggggtcttgc tggtagtcga
    24001 gatcgagacg cacggtctct ccatcggccg tgcggagcag cgccggagcg agcctcagcc
    24061 ggcttccggc gaccgtcagg cgtgcttctt cgaaaactgc cggagggctt cccacgagac
    24121 caattctcgc tcccagcata tcggcgctcc cctgaaggcc gtccgcgtgc gagtaaccga
    24181 agacgccggt catttcacct tcggccgaga gcgtgtcggt gaacgaaaac cccatatgac
    24241 gggagatttc aagcacgctg gcgacaggca gccggttgag agccacgttg actccccaag
    24301 caggatcgga gagataccgt gaagcccgga agcggacgat cagcgcggaa tttcgttcgg
    24361 gcaccgtctc gatctcaagc cgctgcgact tcaggtcaac tcggccccgg aagcgcaacg
    24421 ggcctcccgc ggcgtaaggt ggcatgaggt cccaacgatg gatgtcctga agctgcaggt
    24481 cacccgcgat ctccacgttg tcaatgggcc ctcggaggcg cgccgtggcg gcgacgcggc
    24541 cgtggactcc cagatcctcc ccatgcacca gagtgaccag ttcgccgatt gcgcttcgct
    24601 cgagattcag gctcaggtca agcctgccgt ctcccggggg cgacggctgc cagcgcccgc
    24661 gtcctgagaa ccggccgaag ccgtgcgccg gtcgatctgt gcgcgccggc tcgccggaaa
    24721 agcgaacgtc aaaccggccg cccggaacag aaggcgggtc gatgtcgagg tcggcgccag
    24781 tgaagtaaaa cacggatttc gtatccccga acttgaagtt caggcggcca ccccgaaccc
    24841 ggaactcggg taggtccgct gcagcggcac gggcgacgag cggctggaaa ttccaagggc
    24901 ccgcaaccgt cttgacgagg ttcacactcg gctcgttaag gcgcaagctt gagaactcga
    24961 gctgtccagt gaacaaagct ccgatccgaa tattcgcctc catggaggcg acgtaggcga
    25021 ttggctcgat cccgatcgcg gggtcctcgt ggatcactac tttattcagc gtgaaccccg
    25081 gaccccggag cagactaaag cgaacgtccg cgatctcaac tttccggccg agagcagctt
    25141 cgagtgattg gtggaccctt cgtcgaaact ggtccgcgtg gagaaaaggc gccaccgcca
    25201 cggccgcagc cgccagcatc agggccgcta ggacgacctt ccgaagcgtg acgcgcggca
    25261 gtttcatggc cgcagcggcg ccgacagcaa acggcggtac gcctcggggt cgtcggcatc
    25321 ctcgtggatt cccgggtcgt tcacttccac gcgggcggtc cggtccagat gacgatggac
    25381 gacgtcgcgc gccgtggcgc cggctgcgag cgagaggaac tccggaatca gctcgcgggc
    25441 gcagcagacg gggtgaccgc ggcggccatt ggattgcggg atcacaagca gctcaccgga
    25501 tcggccggag aatcggcgga cgattgcgga gacagtggat gggcgcacgc cggcatggtc
    25561 gaccggggtg aagaggaatc cgtcggcatc ctcaggaacg gcggagaggc cgctctgaag
    25621 agaactgagc tggccgcgcg cgtaatcggg attcaccacg acggcatccg caacctccgg
    25681 cgcgagacca tggcgaacgg tttcggcgtc atgtcccagc accaggatga caggcgcgca
    25741 ccccgcttcc cggaacaatc gagcgagacg ttcggcgaac gtttctcccg ccagcggcag
    25801 aagggccttc ggagtgccca tccggctgga tgcacccgcg gccaggatga gagcggcaag
    25861 tttcacttca gcagcgccct ggtgttttcg tgggcgaaaa ttgattttga gagctcgcgc
    25921 cagtccgacg gagcgttccg ccggacggcg atcatctctg ccacaaccga gatagcgatc
    25981 tcttccggag actcagcgcc aatctccagt cccataggtg cgtagattct ctccaggctc
    26041 tcacgcgcaa caccctccct ctccaattcc ttgatcaccg agatgacttt ccgcttgctg
    26101 ccgatcatgg cgatgtagcg cgcagaagat ccggcgacga gacgaaggac ccgcatgtcg
    26161 tcccggtggc cgcgggtgac gatgacaaca tagccggact cgttgatggc gagccgcggg
    26221 acgacatcct catactcgcc ggcgtggatt tcgtccgcct ccgggaaacg ctcgcggctg
    26281 gcgaacgatt cgcgatcatc gatcacggcg gtggagaagc cggcaagagc ggcgactttg
    26341 gcgaggcttt tcgagatgtg tcccgcccca aaaataaacg cctttggctg agggaatact
    26401 ggctccacga agacctccag ttggccgccg cagatcaggc cgttgtcgta agcggcgtcc
    26461 tgtccaagat tgaaactgag acgtttcgcc ttgcctgact cgatgacttc ccgagcagcg
    26521 ttccagacct ctgcctcaac gcagccgccg ccgatcgtgc cggcgataga accgtcgtcg
    26581 cggatgagca atttggcgct ctcgtagctg gggatggatc cctgtacctg gacaatggtg
    26641 gcaagcgcgc atctcctgcc ttcgcggcgc agccgcacca actcttcgaa gatgtccatt
    26701 cgtcggagat tataccgtgc ccgagcaccg gcagttccga aaccgggtgt gctatcatcc
    26761 gcttcgagtg aagcaagcag gcggtttggc gagcgggctg gcgcttgttt ttgacctgga
    26821 tggagtggtc gtggattcaa atccggtcca cacggaaact tggcgactct atctggagcg
    26881 gcacggggtc gaggctgacg aagacatgcc gtggcgaatg ttcggcagac ggaacgacga
    26941 catcgtccgc gatctgttcg gcccgggcct cagccaagag gaagtgatcg agcacggcgc
    27001 agcaaaggaa cgcttgttcc gcgaggagat gcgggatcag ttgccgcacc gcttggttcc
    27061 cggtctcgtt gcgttcttgg aacggcatcg ggacgcaccc ctcggcgtcg catcgaacgc
    27121 ggaaccggag aatgtcgcgt tcgtgctgga ctgtgccggg cttcggcact acttcaatgt
    27181 ggtggtggac ggccaccagg tacagaagcc gaagccttcg cccgatatat tcttgaaggc
    27241 agccgagttg ctggagacgg cccccggcaa ttgcgtagtg ttcgaggatt cgctcgccgg
    27301 ggtggaggcg gccatcgcgg cgggaatgcg cgtggtcgga gtgtgcacga cgcacggatc
    27361 tttgcccggc gtacagttgg aaatcgagga ctttcgaagc atcgaacttg agtcatggct
    27421 tcgacgtcaa atcccgcttt tgtgaggatg gcaggcctta cggcgatcct gctggtcgga
    27481 attccggcgg caggacaaac gccgctgaca ctggagcaag ccgcctcgag ggaaggcagc
    27541 gactttcgcc cgctgtacga ggggcgagag gtcatcctcc gcgggagagt ctccgtccag
    27601 gtgattccgg tcgtccacta tgcccatctg gcgatccagg acgacaccgg gcacgggttc
    27661 acgattgacg gcccggccga ggaactcgcg cggttctccc cgggggatgt gatcgtagtg
    27721 cggggaaagg tctcggagcg agccggcctg cccgtgctca atccggccga gatccgcatc
    27781 atcgggaagg ggccggttgt cgagccgagg cgcgtcccgg tggaacaact tagcgggttt
    27841 cggcaccaag gcgtctatgt ggtcaccgag ggtctggtcc gtggtgtcgg cacgaacgaa
    27901 ggaggacact ttctgctgct cggcaactcg ggcgctccgg taaaggtctt cctgcccgac
    27961 gcggcaagca cgtccagcgg gggactcaca cagttccggg ttggtgatcg ggtgcgggtg
    28021 actgggatct catcccagta cgctccgctg cctccgttca accggtactt ccagttgatg
    28081 attcccggcg cgggatctgt ggagctggtc gaccggccct ggcccatccc gatctgggcg
    28141 ctgatttcca cacttggtct gctggccacc gccgtgggga tctggtcgat acgcgagcgg
    28201 catatggccg cccgccggcg ggcgatgcgg cggctctatc aactcggcga ggaaatggtg
    28261 ggggcagtct cgccggagga gagtctccgc attctgaatg ctacgttgcc ggagacacta
    28321 ggaatcgtct ccgtgcggat gtatctgtac tcccacagcg agaaacgact ccggcctgtg
    28381 gtggaggccg gcgcggcgcc aagcccgggg ctgctcctgg aggaggcagc cacgtgctgg
    28441 ggaaaccgca gtctgctgtt gatccccgat gtccgccgga gcccgtttca cgcggaaggc
    28501 gccgctgggt tgccgcgtgc cgccctgttt ctgccgatgt tcgcgcagaa agaaccggtc
    28561 ggcgttttag aggttggtga cgcacgaagg ggacgggaat tcagtgcgga cgaactggcc
    28621 gtcgcacagc accttgcgaa tcagatcgcc atcggcatcg aactgaggga gcgacactcg
    28681 atccgcgatg aactcttccg cagcgagaaa ctggccgcaa cggggcaact catctccaac
    28741 gtcgcgggcg agctgaaggc gccattggag gagatcgaga aagccgtcca tcggctgcgt
    28801 ggcagccgcg tggaagtcga aatcatctcg agtcaggcga gccgggcagc cgctattgtg
    28861 gatcggcttc ttgaatttac gcgaaaggat gacgaaagta cccgattcga cctcgcgcag
    28921 gtgctgcaaa cgcttctgaa gaaccgcgag ggggcgtgga cggaaaaaag cgtccgtttc
    28981 gaaaacctgc tgtcttccga tccgatcttc gttaccggct ccggcaagca actggaagaa
    29041 gtcctcctga gcctagtcaa gcacgccgaa gagtcggcgc tgcggacggc ggcaaaaata
    29101 gtgacggttc ggaaaacgaa actcgtccgt ggcgtgcaga tcgagattct tttcagcgac
    29161 ccgggtgaca caggcgcgga tccattcagc gagacaaacg gtgcggacgg ggaggctctc
    29221 gggttgggag tgtgccggag catcctgcgt ggccagggtg gggatctgcg agtcttcaga
    29281 cagcccgacg ggtttctccg gttcgaagtc gaactgccgg ccggtccctc cgcgaacggc
    29341 gactcggcca ccgtctcagc cccaggcgac acccgcatca cgacgctcat attggagccc
    29401 gatgtcgatt cccagcgccg gcttacggcc ctcctgagcg agggcggtca tcgcaccgtg
    29461 cccaccgtca cggcggagga tgctttggaa atcgcccacc ggtttccttt ccatgcaatc
    29521 ttctgctcga cgaggctggc gggcatggac tgcgttgaat tgttggagcg aacgcgcgat
    29581 agaatcgacg cattcgtgtt tctgagcgag tcatatgacg gagaaagcgt tcacgcacta
    29641 ccccatggag aaggttacgt gctgaccaaa ccggtgactt cagaagacct ggatcgcgtt
    29701 ctccgctcca tacaggaaaa agtggcggcc cggtcgtcct gagccaaggc gtcacccgaa
    29761 ttcggccgga gacgaatcgc aggatacaat gagactaacg atggtatccc gaaccgctct
    29821 gaccgctgtg ttccttgcgt tttttccggc gctcaatttc gcggctgaat cttacaacgg
    29881 accgaagcca cccaagccgg acgtcgcttt cctcctgcat gctgacaacc tcgtcgaaac
    29941 cgaaacactc gaagccaaag aggaaacgcg aaaagacgag accatttaca ccatccccgg
    30001 cgccgcctca tccgtgcgga caccgctcgc ggagccgatt tttctgatcc aagtggagaa
    30061 aatcgtgccc gagaagttgt cccttttccg gttggaggtc aaggagggga atcggcgagt
    30121 cgtctttaac cagaagaaga ggaaggacaa ccccgagccg atccggttca tcgcgaccga
    30181 gatcgaggga aagctgtacc gcatcgaggt caaccagcct ctcgagaacg gcgagtattc
    30241 gctgagtccg gcgggatcta accaggtttt ctgttttcag gtctactgag gggcggcccg
    30301 cccaacagtc ctgacctctt caagttgtta gaattcaagt tatatgttca aaggactgga
    30361 gcacactgcg atcgcctcgc caaatccgga gaggctggcg cagtggtacg tggatcatct
    30421 cgggttcatc atcaatcaca ggtacgacgg caacgtcttc gtgaaggcaa agaacggtgg
    30481 gatgctcgag atcatcccgt cggaggggga tcgcgctccc cagaaactca aggacccagg
    30541 gctgcggcat ctggcaatcg ccgtcgacga cttcgaagcg gctcaccggc aactgcgttc
    30601 gctgggtgtc gagttcgcga cagacgtcat gaacctaaag gggaacctgc tcgtgttttt
    30661 cctcgacggc gacgggaact atgtgcacct gatccaccgg ccggcgcctc ttccgtagtc
    30721 tggtggcagc gtggtccaac gagtcttcaa ggcgtttcaa taccgcgact tccgcctgat
    30781 gtggctgggc gcctgcacgt ccagtgttgg cacgtggatg cagaagctcg cccagagctg
    30841 gctggtgctc cagatttccg gctcgccgtt tctgctgggt ctcgattcgt ttttgggcga
    30901 gattcctatt tttctcttct ctttgatcgg cggggtggtg gcggaccgca cggatcgccg
    30961 tcacctgctg ctcgcttcgc agttcgtgca gctgagttgt gcgttcctgc tggcggggtt
    31021 gatcgtgttc gacgtggtga aggtctggca catcctcacg ctctccttcg ttgtggggct
    31081 ggcccaagcg tttggcgggc ccgcctacca ggcgctcatc ccgtcgctgg ttcgttcgga
    31141 ggacctttcc aacgcaatcg ccctgaactc gattcagttt aatctcgcac gcgtgatcgg
    31201 cccgatgctc ggcgggctcg cgctgacgaa gctgggcgcg gcctggtgtt tcgggctgaa
    31261 cggcgtctct tttgtcgcgg tgatcatttc cctgctcatg cttcaaatcc ggtttgtacc
    31321 ggagcggacg aatgagtcga tgctcaccag catgaaacag ggcatccaat tcattcggag
    31381 gcagagagcg atggagccgt taatcgcgct cgcctttctg atgacgctgc tcggcattcc
    31441 gctgatcgtc tttctgcccg tcttcgccaa agacgtgttc caccacggcg cgaataccta
    31501 cacgctattc ctgtcaatct cgggggcggg ctcggtgctg ggtgcgctgg tggtcgctgg
    31561 gatgggcaac attcgccaca aggggcgaac cgcgctgttg atgctggtcg cgttgggaat
    31621 ctgcatgggc ggtttcgcac tttcgaagtc gctggtgctg agctgcctgc tgctgtttac
    31681 tgcgggcatt gcgctgatct cggtgttctc gctggtcacg tctctcgtgc agttgatcgc
    31741 cgtggacgag atgcgcggcc gtgtgatgag cgtgtataac gtggccttcc gcggtgggat
    31801 gccgatcggc agcctggtca cgggatctct tgttccggtc ttctcggcgc cttccgtgtt
    31861 cgcctgcaac ggcgtgttac tgaccgcgct tggcctctac ttccttctgg tgcagaggcg
    31921 ggtggcatcc ctatagaatt gaggtttatg cgcgcggcgg caatcattgg gacactcttg
    31981 ttggcgccgt ggctggtggc gtcggaactc gaggctgctc gagatatgca ggacgcggcg
    32041 aaactagagg cgctgatcgc ccctctggcg gatgccgccg ccaggagtac aaacgacgct
    32101 ccggcacagt accggctggc gctggcccga tcctatctcg cggaggtggc catggagctc
    32161 cgcgacaaga cgcgggcgaa ggtggcggcc gaagcgggca tccaggcagc cgaacgcgcc
    32221 gtagccttgg cgggcaatgt ggcggagcac caccggattc tcggaaccct gtgcggacaa
    32281 gtgattccgg ccaacgttct cttcggcttg aagtacgggc gctgtgcgac ggattcggtc
    32341 aacaaggcga tcgaactcga cccgaaatcc tcctccgcct acctgagccg cgcggtgggg
    32401 aactactacc tgcccgaggg actcggagga ggcgctcaac tggcgctgcg ggactgccag
    32461 aaggctatcg agttgaatcc gaaatcggct gaagcgcacc tctggctcgg catcatgctg
    32521 cgtaaactga atcgaaacga ggaagcccgg acagccatcc gaagatccct ggagttgaat
    32581 ccgcggcggg tgtgggcaaa gcaacagttg gagaagacgc cgaccaaata atgagaaaat
    32641 ccttgccggt cgcggcggcg ctcatccttc tgaccgccgt tgggtacctc gagtttccgg
    32701 gccacaccta cctcttatcg gacacgcaga tctacattcc catcctggag cactaccgcg
    32761 acccaagcct gttcacccga gagatggtcg cggtccgtcc ccacgtcgcg ttcacgatct
    32821 atgacgagat ggcgctgggt cttcggcact tcaccgggct cggttttgag caggttctga
    32881 cgatccagca actcctcttc cgggcgctcg gcatcttagg cgttttcttg atcgctactt
    32941 cgatgggcct ctcgcggcag atggccttcg tcgtttcctc cgttttcact ttgggagcga
    33001 cgatcggcgg tcccaccgtg ctgacgttcg aatatgagcc gattcctcgc ggattcgccg
    33061 ttcccctttt gctccttgca gccgggatgg tagcccacgg gcgcgacttc ggtgcgggga
    33121 tcgccgcggc gctcgcgttt ctctaccatc cgcccactgt ctacaccttc tggggcgtgt
    33181 attttctgct gaccctttgg ccgaacaagc ctgcggtaat gagccggcgc atctggggac
    33241 tgctgcccat gctgtgcgcg gtggtcgtgc tcttgtttct ttcgcggctt caggccggtg
    33301 tcaccgaagc acagcccttc ttcagccgta tcagcccgga actggagaag cttcagcgga
    33361 tgagggctcc gtacaactgg gtatctctgt ggttccccta ctggcactgg ctgtccgtct
    33421 ttctgtgggc agtctcccta gcggcctact ggcgtgtgcg aagatgggcg cccgaggatt
    33481 tgcggttttt ccttgtgggt ctgccgttgc tcggtattct gagcctcccg ctgtctcacg
    33541 tactgcttga gaaactcgcg tggatcctga ttcctcaggc acagccggcg cgggccgtgc
    33601 tgttcgtaac ggtgtttgcg ggaattctgg catcagtggc gggagtcaag gccgtggagg
    33661 cggggcggcg gattgaaggc gttatctggt ttcttcttgc cttcgcgatc ccgatacaca
    33721 cccgggttac ggagttgctg ctgccggact tgagcgagcc ggtaattcgc cgacgtctcc
    33781 tgctggccgt cgcattctcg ggagtggctg ttctggccgc ctgggcggcc tcggtgggcc
    33841 gaaagtgggc gccggcagcg atggttctcg tgagtctcgc gcctttctgg ctgataccgt
    33901 cctacggaaa cgttgtcacc tatcgtgaag ttcgaacccc ggagctattg gaactgtgca
    33961 actgggccag gcaggagact gcgaaggacg ccatgttcct tttccccaat gccggccgtt
    34021 cgctggagcc ggggatcttc cgcgccacgg ctttgcgggc cgtgtacgtc gactggaaag
    34081 cgggagggca agtcaatttc ttcccgaagc tcggcttcga atggtgggaa agatggcggc
    34141 aaaccatggc acgcccgttc cgtccggaga agcttccgct tttcgcgtca ctgggtattg
    34201 actttatcgt gcttccagcg aagaaccggc taaaagacgg cgcccctgtc tattcgaacg
    34261 ccggcttcgt ggtatacgcc ttacggaagt gagcgaagtg gccgcgccta tcggctcaac
    34321 aggaactctt ccacttcgcg gcgcgagggc accgaagcct gagcacccag gcgggtgacg
    34381 ctgatcgccg ctgccccgtt ggcgaagcga agcgcctcct ccatcggctt gccttcggcg
    34441 agggccacag caaggccggc gttgaacgta tcacccgcgg cggttgtatc gcgcgcctcg
    34501 actggaaagc tgtgtgcttg cgtcccgcgc tcgccgtcga ggcagaagca gccctgctcg
    34561 ccgagcttca ggatcaccgt gcgggggccg agcgcctgga gatcgtgagc cagcgcggga
    34621 gcgtcggcct gtgagacccg cgtgaccggc ctgccgagca gatggcacgc ctcgctttcg
    34681 ttcggcgtga ggatgtcgac ggcggcgaga aactcgcggg acagcggtcg agccggcgcc
    34741 ggatcgagga ttgtccgcgc gccttcctcg cgcgcggcca caagggccag ttccaccgta
    34801 tcgagagggg tctcgagttg gaacagcacg aatcgcgccc cgcggaacga ctgccgcatg
    34861 gcgccgacat cgttcggcag caacgcatga ttcgcgcccg atgcgacaac aatcgaattc
    34921 tgacccgagc ggtcgaccca gatcagcgcc acgccggtcg gctgggattt cgttgcgtgg
    34981 actgcagtca catcgactcc ggccgctgag aggctcgcct tcagatgatc ggcgaacaga
    35041 tcgtaaccca cgcggccggc catgcgcacc gaaaccgcgg gcccgccgag tttgccggcg
    35101 gcgcacgcct ggtttgcgcc cttgccgccg ggaatcatct ggaaatcgcg cccgagcacg
    35161 gtctcgccgg gagcaggcag gtgttccacc tggaccacaa aatccatgtt gaggctgccg
    35221 acaaccacga gggagggctt catcgggaga ggtcgatgcc gagtttcttc atttcgtcag
    35281 cgtaatagcg ccggtgcagt agatcccact cctcggtccc ctcttgaatc tcgcgcttct
    35341 gcgacttgat tttcagacga gcggcacgat cgactcggtc ctcgacctgc aggagctccg
    35401 tgatctgctg cacaatctcg agcctcacgt cgttggcatc tcgctttacc cggatctccc
    35461 gagctttgcg cagcgccgag atcagcttgt gcgagatctc gttgattttg tccctggaaa
    35521 gcttcatccg gtcgccggta tccctacccg acgcccgcac caccttgcgc tgtgagatca
    35581 gcgtgttctt gatacgcctg aacatttcct ggtagctgac cccctcgcgc cgcatgtaat
    35641 cgctatactg gctcaggatt tccctgactt cgtcgttcaa acggtcttcg acctgaagtt
    35701 cgtcggtgat gacactcgag accagttcgg tggctgcctg ggggttggtg gtttcgatga
    35761 cttggggact cagtttggtg accaactggc gggacagata gaagattagt tccctgggaa
    35821 gaagcatccg ttggttgttt cgctcctcag catcgactca atcaacagtc tactgtccgc
    35881 tgtgcgatgg tgtcaaaccg agcggtggtg gcgcgagtgc tgacagcggc tgctgacagg
    35941 atccgggcga cgtttccgtc ccatgcccta taatcagcat ttgcgcggtg tcgtgactca
    36001 aaacgttatt ggcctaagcc tgagcggggg cgtctgagtg gaacgcggat cttccacctg
    36061 gctggccgta gccaaggcgg cgatggatgt ggaagcgagt gctatctccc tcgcggcaag
    36121 acggctcggc gagaacctgt gtcaagcggt ggagttgatc ttggaccgcc ccgggaaggt
    36181 ggtcgtgacc ggagtcggca aatccgggca ggtggcgcag aagttagcgg ccaccttgtg
    36241 tagcaccggc atctctgccg tattcatgca tgccggcgac gccgttcacg gagaccttgg
    36301 ggtcctcgcg cccggagatc cgtgtttgct ggtctcaaag agcggcgcca ctgccgaact
    36361 gatccgccta gtacctgtgc tgcggcaagt tcaatgccct ttgatcggga tcatcggtga
    36421 tgcgaattcc cccctggccc gcgacgtgga tgttttgctg gatgcttccg tgagcaggga
    36481 ggccgacccg gagaatcccg tgcctactgc cagtacagcc gtagcgatgg ccctcggtga
    36541 cgcactggcg gtggccttga tgcacgcacg gaacttcact gtggacgatt ttggccgtca
    36601 tcaccccggg ggacaactag gacgtaacct ccggcttcaa gtgaaggccg ccatgcactg
    36661 cggggaagag gttgcatggg cgcatcggga cgattcgttg aaagcggtcg tgatcgcgat
    36721 gacacgccgg ccggtgggcg ccgcttgcgt ggtccgagaa gatgggcgtc tggaagggct
    36781 gatcacggat ggcgatcttc gccgagcgct cgaggcgcat gatgacatcc gcggcttaag
    36841 ggcggccgac gtaatgagcg gaccaccggt ggtggtctcc ccggacgccc gcttggcgga
    36901 tgctttggcg ctgatggaga accggccccg gcagatctcc gtccttcccg ttgtcgaacc
    36961 ggcgagcggg cgctgccttg gcttgctccg gctgcacgac atctaccagg tgggcaatgc
    37021 ctgaggtgac ttccgcttcc gctcccgctc ccattacgct ggccgatgcc tcggcgcgca
    37081 ccgtcgtctt tggcggcggc cgtccactgg cattgattgc gggaccctgc gtgatcgaga
    37141 gtgaagagca cgtgcatttc ctggctcaac agatctcctc catcgccggg ccattcgtct
    37201 tcaaggcctc gttcgacaaa gcgaatcgca gcagtctatc ctcttaccgc ggtccgggca
    37261 tcaaggaggg tctgcggatc ctgggtgcac tcaaggccgc gggccacttc atccttacgg
    37321 atatccacga acccgaccag gccgcccagg cagcggaggt cgccgatatt gtccaggtcc
    37381 ccgcgttctt gtgccggcag accgatcttc ttctggaggc cgggcggacc ggacggatcg
    37441 tcaatctgaa gaaggggcag tttgtggcgc ctcacgattt cagattggcg gtcgaaaagg
    37501 ttgcctcgac agggaacaca aagatcctgc tcaccgagcg cggcgtttgt ttcggctaca
    37561 acaatctggt ggtagatatg aggggcttgg tgatcatgcg tagcttcggc ttcccggtca
    37621 tcttcgacgc cactcacagc gtccaattgc cgggaggcgc cgggagttcc tcagacggcc
    37681 aggctcaatt catcgagccc ctggcgcgag cggcgacagc cgtcggaatc gatggcctct
    37741 ttgtggaggt ccataacgcg cccgaaaagg cgctgtccga tgggccaaat gctcttcgtc
    37801 tgaatctgct tggagccctg cttgaaagac tgcggtccat cgacgcgctc gtgaagaccc
    37861 aacgtcccta gtttggcggc tccccccgat ggttggccag gaaatccgcg tcgatccgct
    37921 gacgtccccg gaatccgtcc tggatcctgt gccagtaccc gatgccaggc tcaccgagct
    37981 tccaacaaag atacacctct tcgtcacgga agagcgtcgg aaaatcgaga aggccgttgt
    38041 ccagatcctt caccaggcaa ccgaattcct ggattgcatt gatcagttcc ttgagccgtg
    38101 cggcgagggc gtcgcggcgg ccgcggacgt ggatgagttg ctcacggttc accaccgccc
    38161 cgccggacat ggtgacgcgt tgcacgacgg cgttcagttc agcctcagca tcggcatatt
    38221 ggggcttgat ggagaccgcc tgccgcagat ccgcctccac cgccggcaga atgctttcgg
    38281 cttcttgtac ggtgaagagt cgtgtcgcca tgtggggcgc cgcttagata cccgagactt
    38341 ggacggcgag gctgcgggta cgcggcccgt cgaactcggc gaagatgatg ctttgccacg
    38401 taccgagcag cacgctggca ttccttacct gaagacagag cgtctgcccc aacagcatcc
    38461 cgcgaagatg gctgtcggcg ttggaccgct cgcagtcgga atgctcgggg tcgttgtggc
    38521 gccagtactg gtcatgctgc accatctggc taaggaatgt cttcacgtcg tggcggagag
    38581 cgtcctgcca ttcgttcatg aagacagccg tcgtcgtgtg gagcgattgc aagtgaacca
    38641 ggccatcgcg aatgccgctc tttcggacga tctcattcac acggtcggtg acattcagca
    38701 actccatccg ttcagtggtg atccagtcga caatccgact gaaaacccgg taaccggttt
    38761 ggacacccgt ccggcctccg ttgggttcga tcgtggcgtc ggccggggtt tcggtattgg
    38821 tggtgggcat cagcgctcct gtgctcgtct tggatatccg aaataccagt aagatccagc
    38881 taacctggac aattgccctc attataccaa gtgctctgct gtccgactct tggctgtgct
    38941 attaaaggag atcgcgaaga ccatggacgc catcaacgca ttgaagactc gccgctcggt
    39001 tcgtgcctac cgccgcgaac cgattcccag agaggccctt accgacctga ttgactgcgc
    39061 acgcctcgcc ccaacggcaa tcaacatcca gccatgggag ttcgtggtgg taaccgagcg
    39121 ggaaacgctt aattcgattg ccggtacaac cgatcacggc cgctttatcg cggacgccgc
    39181 ggcttgcgtg gttgtgctat gtcaagacac aaagtacttc ctcgaggacg gcagttccgc
    39241 cacactcagc atccttctcg cagcccatgc tcacgggctc gcgggctgct gggtggcagg
    39301 tgacaagaag ccctatgcag ccgaggtttg ccggatcgtc ggtgcaccgg ccaactaccg
    39361 ccttatcagc ctggtcccga ttgggtaccc ggcagagcag cccgagaaag agaaacggcc
    39421 gctctccgat gttttgcact gggaaaaatt ctaactgccg cgggcttcct atttgttcta
    39481 ctctggtaca gaaggggctg gcggtcccgc ccgaaagctt tctacacgcg accctgcctc
    39541 aactgggaga tttgcatgtc caacaagaga acaattgccg tcgtcggggc aaccggcggc
    39601 caggggggtg gtctggtccg ctccatcctg ggcgatccga acagcggctt caccgcgcgc
    39661 gccctgacaa gagacgttag ttccgcgaaa gctaaagcgc tggagaagct tggcgccgag
    39721 gtcgtctcag ccgacgtgaa tgacacggag agcctgaaga aggcgttctc gggcgcgcac
    39781 ggggcgtatt gcgtcacgtt ctactgggac cacttctcgc cggagaagga aatggcgcat
    39841 gccgccgcga tggcccaggc cgccaaggac acaggcgtca aacacgtgat ctggtccacg
    39901 ctcgaggaca cgcgcaagcg tgtgccgctg agcgacggcc gcatgcctac cctcatgggc
    39961 aagtacaagg tgccgcactt tgacgccaag ggcgaggcga accatttctt caccgacctc
    40021 ggccttccgg tgactttcct gctgacgtcc ttctactggg acaacctgat ccacttcggc
    40081 atgggcccga agaccggttc ggacgggaaa ctggaaatca ccatgccgat gggtgacaag
    40141 aagcttcccg gcatcgccgt cgaggacatc ggtaagtgcg cgcacggcat cttcaagaag
    40201 gggcaggatc tgatcggcaa gacggtcggc atcgccggcg agcacctgac cggttcccag
    40261 atggccgccg cgctgacgaa ggctctcggc cgagaagtcc gctacaacga cgttccgccc
    40321 gaggtctaca ggagcttcgg attccccggg gccgacgacc tcggcaacat gttccagttc
    40381 aagcgcgatt tcgagacgga gtactgtggt gcacggaacc ccgaagtggc ccgctctctg
    40441 aacccgtcgc ttcagacctt cgcgatgtgg ctcgccgagc acaagagccg catcccgctg
    40501 gaatagcggt cataaggaac cgcaaccaaa gcgggcgact gaattgacag cttctcgccg
    40561 ccctgtcaaa cttccaagga ttgcattttc ggaggtatgc gcatgcgtct cgccagaggt
    40621 ttctccccgg cggcgggaac tttgattctg tgtctgtgca cggcgcgggc tctgtccggc
    40681 caggcgcccg caccatccaa ccgaatcacg atcgaccccg caaagcagtt ccaggtcatc
    40741 gaaggcttcg gactcaacta cacgggcccc tatttccgaa acgatcagaa acccatgttc
    40801 gacatgctcg tcaaggacct cggtgcgagc atgttccggg tggtcgccta tttcgtgtac
    40861 agcgactggg agtccaccaa cgacaacgat gatccgcatt ccgcgaattg ggaatactac
    40921 aattcgcgct attcgaaccc cgttttcgaa gcgacgtgga acggccttcg gcacttgaat
    40981 tcgatgggta tccggccggt actcgctctt atgggacccg tccccgactg gatggtcgag
    41041 gagggaacgc ctcctccgca gcacaaggtt tgccagagtt catccaaact cggccgcctg
    41101 aagccggaga tgtacgatga attcgccgag acagtcgtca ccatggccgc ctatgcgcgg
    41161 cggaaagcag gtgtcgactt cgatctcttc agtccgttca acgaaacgga ctgctatccg
    41221 cccgaggggc cgcgcgtgga tccggatgaa gttgcgaagg tcctgactgc ggttgcccgc
    41281 cgcatgaacc aggaaggtct tggcgatgtc cgtcttgcgg tcgccgacca cgccatcatc
    41341 acgaccgact atatcagccc ggtcctgaag gacgccgact tgatgaaaca ggtcggcgca
    41401 ttcacggttc acacatatgg tgaggactcg gtcggtcccc atgtgaagaa cgtcgcccgg
    41461 agtgcgtttc gcaatgtccc ggtctggctg accgaatacg gcgacctgaa cgaccaggac
    41521 caaacgttcg agaacgaatg gaaacggtac tctctcggcg ctaaccgccg cgctctcacg
    41581 gctcttaatc agggcgccgg agcgctgttc tatttcaacg ccttcgatga ctacgaggaa
    41641 tgcgcccgcc gcctgacgta ttacggtctc tttcgcagcg ccggccaggt ttactcgccg
    41701 aagaagcgat actacgcaac gaagcagctg taccacttcg tcaagccggg cgcacggagg
    41761 atcgccgtga cgacggacgc tcagggcgtg accatctcag cgttccagga cggcgaaagt
    41821 gattcgctgg tcgttgttgg cgtcaagcag gggggcccaa atcggctgaa gatcaccttg
    41881 ccggcgccca gccgcgcgcc aagaagttgg gacttgtaca tgacgacgca cttgctcgat
    41941 tgccggaagg tacaaacgat ccagaacgcg ggtccatccc tggagatcga actgccggac
    42001 gaggcagtct tcaccttgac cggcagattt tagccttttg gctggttcag cctgttcagg
    42061 ctatttccgc ggatggatcg cttgttccgc cttctcgcgg ctgaagtcgg cgggcatcgc
    42121 gccctccgca gtctcgaaca actccttcca cgacggcgtc ttcgaagcga tcccgtcacg
    42181 gggaatgtca acacccgcaa tccaggcgat cccgttcaac agcactctgc ggtagttatc
    42241 gtccgccaga gcccagtggt agtgcgcgcc ggtgaaaccg aagccccgcc cgccgccggg
    42301 acgatcgtac gcccacgcca cgtgctccgc catccccttg cgcgagcgga ccacgggatt
    42361 tcccgagtgc gggccgtccg gtccttgccg ggttgcgtcc ggaggcaccg cagttaagat
    42421 cggagtcacg ccgtccatgt tttcgcggaa gcgcatgtgg tagtaccatt cgtcgcgcat
    42481 cgtgaaggga cgtacgccgc gagtcaccgg atgttcgggc aaccgttcga aacgggcggt
    42541 ccagatggga tttaccgacc agtaggtttc gtagtaaccg ccgatccact ggagcaactt
    42601 atcgccgggc tcaccttcgg gagctaccaa cgcatagtgc agcacggcca ggcccacgcc
    42661 tttcttcatc agcgcgtcga gcgcctcaag atgaggcagc atcgggtgct tgtcgccgcc
    42721 gtccatgtag atcacaacgg cgctcgcatt atcaaagtaa gacggctcgg ccggccagcc
    42781 gtcccggtgg actatcgcct ccacgcccgg caaactctcg ttgaggcgct tcgccagaaa
    42841 taggcagcct gcgttgtggg aatgctcacc gtaaccgtgc gtcttcacgc cggcgatgaa
    42901 cacgatccgc ttcttcggag cccccgacgc actcagcccc gccagagtgc caacgaaagt
    42961 ccggcgagag aacagcgggc ggccatcaga gcacaggaag tcactggaca tgaaccgtac
    43021 tctaacatcg ttcaggcgga gtgttccggc ggcgttagcg cccaacgccg aaccacggca
    43081 tgctggtcag gttgacattg atggcgagat cagcgggtgt tcccggcgag ggcgtccggc
    43141 ggcgcggtcg caggttccga atcgtgccgg tctccatcac aaagccgtcc cctgccggaa
    43201 ggtcctcaag atggcggttc aatcatcggc ggcccgacaa agatgaggtc gttctcacga
    43261 atatcgtctc aagttcagac attcccccgt tttacccaca tgcccgactt tcgttgcggg
    43321 agcagtttgc acagaagaaa cgctccggtc gcttcccgtc tacagcaccg cagaacgcaa
    43381 acgaccacag gtcgggcaag ggcgacagca actacgacat ccggcagacg atgacaacca
    43441 atggggtctg gatgttgccg ttcgggccgg gtcagcgatt cctacaaagc cagggattcg
    43501 cgggaaagct ggtgggccgg tgggaactga gcgcctttca cgccgcgcgc acgggaaggg
    43561 cgactaactt ctccataagc cgcagctcga aagatctgcc ggacggtaac agcagcaacc
    43621 agcgtcccga cgttgtgccc ggcgtttcca tctacccgga acaccagacc ctcgacaact
    43681 ggttcaacat cgccgctttc gcggttccag ccaaggggac atacggatca ccacgattct
    43741 gttgtgcctg tctctgcctg gctggggcga aacccgtctg tgacggccat tgagccggcc
    43801 gttgcgacgg caccagtcgg tgaggatccg gacgacccgg ctgtctgggt caacccctcc
    43861 gatccggccc gaagcctggt cctcgggacg aacaagaccg gcgctccgca aggggcactg
    43921 tatgtcttcg atctgaaagg caagatgctc cagaagatct ctaacctcga ccgtccgaac
    43981 aacatggaca tcgagtacgg gttggtcctg aacgggcagc ccaccgacat cgctgtactc
    44041 acggagcgtt accggcagag gcttcgcgtc ttccggatcg catccgacgg ttccggtctg
    44101 atgcgctatt tcctcgggcg gcggcatgcg cgtgtttgag gaccagcaag gggaagtcag
    44161 ccacccccat gggtatcgct ctctaccgcc gtccgcggga tggcggtctt cgccaatgtc
    44221 ggccggaaga ctggggccca agcaaggcta tctctggcag tacaggctgg aggacgacgg
    44281 ggtgggcctg gtgaaaggca caaaggtccg agagttcggc cgattcagcg gaaccggtga
    44341 agttgaggcg atcacggtcg atgacgccgt cgggtacgtg tactacgccg acgagggcga
    44401 cggcatccac aaatggcacg cagacccaga ccatcccatg gctacccgcg aactggcgca
    44461 tttcggccga aagggattca aggctgaccg ggagggaatc gcaatctatg cgcgtcccga
    44521 cggaaccggg taccttcttt gcaccgatca actgcctggc acgagtgagt accgcgtgta
    44581 tcgcagggag ggcaaggccg gaagacccca tgaccattcc taggtggtga atattcttcg
    44641 tggcccagcc gactccaccg atgggctcga ggcgacctct gccccacttg gtccgcagtt
    44701 tgcccgcggt ctgctggtgg ctatgaacag cggcgggaag agctttccgg ttttctcgtt
    44761 cccggagttt tgagcggtcg ccggcagcag acacacgtcg agccgttccc tacaccgtcg
    44821 gttcgccgtg gacctcgatt tcgtcggggt cggtgccggc ctctggcggc tcggggagcc
    44881 ggccgctgag gtgagcccag gcccaaaaca cggcggtcga ggaactgagt acgccggacc
    44941 agacgccgat tgcgcgcacc gtcatcgatt cggaagcgat cccggctccc atcatcgaaa
    45001 gcatcatcgt cgaccacgtc atcgattcga gcgtggagaa cacccggccg cgaaactccc
    45061 cggatacgtg gcgtagcaac tgcgagaaat tcaggatcga agacaccgcc accgcggccc
    45121 gtgacagcgc gatgaagacc agcgccagcc agaatggttt ggagagcgcg aacaggacgt
    45181 acgccgcgcc gtggatcacg tagcagacgg caatcgtcca cttatagctc tcgaagctga
    45241 tgcgcttgcc catccagtga ccgatccctc cgccgactag gagtccgagg ccggcgaaac
    45301 tccagaggat gccgataccg gccgcacccc gcttgaaaac gagttctccg aagagactga
    45361 acaggatctg cgcagcgccg cctccagtcg cccaaccgac atggattaac gcgatgccga
    45421 gaatgagcgg gctggcgcgc atgtagcgca gtccctcggc atactcgtgc caaggccgga
    45481 cgacttctgt ctcggtcagc gccgtccgct tcgaccggaa tccgcccttc ggcatcctca
    45541 gactggaaat tgcccaagcc gagaacagga atgacaggaa gttaagaaaa aaggcccact
    45601 cgtaaccaag atgcgcggcg ctcaagcctg ccgtcatcgt gcctatggtg agcgttgtcc
    45661 attgagtagt ctgtgtcagc gagttggcag tgtgcagttc ctcgcggctg gcgatggttg
    45721 gaagaatcga cgcgcggccg ctggtaaaga acggcgaggc gaacatgagc aggccgctga
    45781 gaacgtagag aagccacggc tcgggccgcg acacggtaaa gatgaacatc agggcgactg
    45841 ccgcgcggag cagatcgctg atgatcatga tgcgcttgcg atccatgcga tcgagcagaa
    45901 cgcccgcaag cggcccggca agcacgaccg ccagcccgcg agacagcatg atgccggtga
    45961 cgaccatgcc ggatccggtg cgctcgacag cgagactgaa gaccgcaatg ttgttgaagt
    46021 ggtcgccgat ctcacttact acctgtccca tccaggtata gcggtaattc cggttctgcc
    46081 cgagaagccg ccgaaagccc gccatcaggc ccgctccgcg atgaaaacgc cggtcagcac
    46141 aagcgcaccg ccggtgacga ggccggcggt gaccggctcg ccgaagatga gcgccgctgc
    46201 ggatgtcgcc agcaagggct gaaggtaact gaacgccgag acacgcgagg caggtatgta
    46261 agtaagtgcg tagtaaaaga tcaggtaaca gacaagtgat gggaaaacgg ccatgtacac
    46321 gaagctgagc caggctttca cgctcacctg ggcgaaggag aaatcccagg cgtaccagag
    46381 cgtcagcggc gaaagcatca ccgcgccgcc aacataagcg aacatgttca ccgtcacgct
    46441 gccgtgccgc gccgtgatct gcttgccgaa gaccgtgaag aacgcgaacg agagcgcggc
    46501 catcagaatc caaccgtcac cgaacaacga ggcgccggag gtcttcgcgg gcgctacgct
    46561 cagcaccgtc acgcctccga tggcggtcag catgccggcg acacgccttc ccgtgatccg
    46621 ctcctgaccg acaattccgg cgagaagcag cacgaacagg ggagtctgcg acatcaggat
    46681 ggacgagtgc gcgacgctcg tgttgttgag gccaatgacg aaactgagct ggttcacggc
    46741 cacacccatc aaccccagga acaggaagcg gggcaactcg tcccggtccc aattcatgcg
    46801 gacgggatcg cggcggcgca tccagaggta gaccggtaga atgagcgccc cggcgagcga
    46861 cacacgcatg ccgccaagaa gtaggggcgg aatctcccgc agcgcgacct taccgatcag
    46921 gaaattcatc gcccagaaca acaccatcaa gacgatcaat gagtagaggc gcagcgcgga
    46981 gggctgcgcc ccccgatgcg ggtcgggttc gctcaaccgg cggcctcctc gcgaatggtg
    47041 atggagcgga tgaagtcgag gtcgagcttg taaccgaagc cggcgccttc tcgaacctcg
    47101 atcgtgcccc gcggtgtgac ttccactggc gggacaatca cgtcccgcga ccagtaccgc
    47161 ttgctggcgg agacgtctcc aggcaacacg aagttcggta gcgtcgagag cgcaatgttg
    47221 tgggcgcgcc cgacgcccga ctcgagcatg ccgccgcacc agactggcac gccggcggcc
    47281 tgtgccacgt cgtggacccg cctggcttcg gcgaagccgc cgacgcggcc aagcttgatg
    47341 ttgataattc ggcaggcgcc gaggcggatg gcctgctcgg cgtgatgggc tgtgcggatg
    47401 cattcatcga ggcagatcgg cgtgtcgagc cttcgttgaa gctcggcgtg gtcgatgatg
    47461 tcgtcgtgcg cgagcggctg ctcgatcatc atcaggtaga actcgtcaag gcgcttcagg
    47521 cggtccgcgt cggcgagtgt gtaagccgag ttcgcgtcgg ccatcagctt gatgcccggc
    47581 cagcgttcac gcaccgcgcg aacgacgtcc acgtcccagc ccggcttgat cttcatcttg
    47641 atccgctggt agccggcggt gagctctgtt tcgatcttgc cgagcagttg ctcgaccgaa
    47701 ttctggatgc cgatcgacac gccacaggag atttccttgt gtgcaccgcc gccgatgcgc
    47761 ttccagagcg gctcgccggc aagacgtgcc tcgaggtccc acagcgcaac ctccagaccg
    47821 gcgcacgcca tgcggtggcc gcggatgtgc ccggtcagcg gagcggcttc caccgcgctt
    47881 tggagcgtcc ggccctgtat acgcggaatg gcatagtcgc gcaggatcat ccatgcggat
    47941 tccgtccact cctcgttata gaaagggttc tcacccgcgg taacttcgcc ccagccggag
    48001 acgccgccgg cgatggcttc aacaaagatg atcttccgct ctgtggtgcg accgaagctg
    48061 gtctcaaaga agtggaccag cggcatgcgc acgaggcgca gaatgactcg ctctactcta
    48121 atttccattg gcctaacagg tagatacctt ccttctctgt gcgctcaaac ccaacgacgg
    48181 cgagactccg ttcgaaacca cggaggaatt gctcgctgac ggacttctga acttcacggg
    48241 cctgcttggg gtcctcgcgg cggattgtct ggatggccgc gggaaccacg atttgtcctt
    48301 caatctccgg tatggggggg cgctgtccgg acagcagcgc ttcgacgtag aggctcgaga
    48361 tccaccactc ggcgacgcag cggtcggtgg gcattcccga gtggagggcg ctagtggtgg
    48421 taccgtactg gttcagcacg aaacggcgga caacggcgcc aagccgctcg atgttgaagt
    48481 aagcgttctt cagctccagc gggtcgaaag tccactcgat cagctcgata ccgcgtagca
    48541 tggcgtcatt gcgctgctcg agcttgagca tgcggccgat gccacggttg gtgtagtcgg
    48601 gcagcactcc gagcatgtgg ctgtgcaggt atgacagccc gtttgacttc aggcccggaa
    48661 tcgcaaggca gaaggcgatc atgcgaccgc cttcaaaggc gcccaaggct tggcccccga
    48721 tcttggtggc aacgacgaac aggcgcacgg gcagcaattc gatgtcgctg aaactccaga
    48781 tctgctgctg gagccgaacg gcgtctccga attcggcgtg tgtgcgcagc ggcctcacct
    48841 cgatcacgtt tgtcgtttcg cttcctccca tagttgatcc atctcctcga gtgttgcttc
    48901 ggaaagcggg cggccccggg cgttgagctt tcgctcaagg tagccgaagc ggccgcgaaa
    48961 cttggcgttt gtgccgcgta gcgcctgctc ggcgtccacc ttcagcagac gggcaagatt
    49021 cactactgcg aacagaaggt cgccgagttc gtgctctatc tcgtcgggaa ggccgcggcg
    49081 gcgggactcg gcgagctcct tcagctcttc atcgagcttg gcgagtacct ggtccgggtt
    49141 ctcccagtca aaaccgacgg tagaggcgcg agacgtaagc tgttgcgcct ccacgagggc
    49201 cggcatcgag cgcggtacgt tgtcgagaag tcccggccgg ctcgcaggtt tctcgcggtt
    49261 ctcttgccgc ttgatctcgt cccagcgctg cttgacctca ccggcggtct tggcgtcgcc
    49321 gtccgcgaaa acgtgcgggt ggcggcggac gagcttttcc acgatggcgt cgagggagtc
    49381 ctcgatggag aatgtgccct cctcctcggc gatgcgggct aagaacacgg cctggagcat
    49441 aagatcgcca agttcatcgg cgagcgcctt ccagtctcgg ctgtcgatgg catcgagaac
    49501 ctcataggtt tcttcgagcg tgtacggctt gatcgagtcg aaggtctgct cccggtccca
    49561 aggacagccg tccggtgcgc gcaagcgggc catgatttcg accagtcgcc gaaacttctc
    49621 cccagcggcc gaagaacgtg cgtttgccat atgccggaag ctcactattg tcgcattttc
    49681 accccagccg gctagcaaac cgggggtgcg cggtagaatg gaatgcaatg gaagagaagt
    49741 ttgtaagcct agtccttttt cttcgctttc aggtggagct tgctctcgga ttctatgggg
    49801 cgccggacgg caagacagct ccggagccac aattcgagat cgcgcggcac tttctcgacc
    49861 tgctgggctc gctgcaggaa aagacgaagg gcaacctgac cctggaagaa gatcgggcgc
    49921 tgaataacaa cctcaccgag ttgcgattcc gctacgtgca ggcgttcgag gagagcaaga
    49981 agaagccaca agccgaagag gcaaagccgg ctgaaccgca gccggctggt gagcaggagc
    50041 agcagtcctc gtagcggatg tgcggcccgc aaccagaagc gatgacgatc acggttcttg
    50101 gctcgggaac cagcgtgggt gtgccgacgc tcggatgcca ctgcgcggtc tgccggtccg
    50161 aagactctcg tgacaagcgg ctgcgcccgt cggttgccct gcgctacagc ggacgcgtgg
    50221 tcttgatcga cacgacaccc gactttcgct accaggcact gcgcgctggc atcgagcgga
    50281 tcgacgcgat catttacaca cactcgcacg cggaccacat cctgggcctt gacgatgtac
    50341 ggccgttcaa cttccggcag cgcggcagca tcccgatcta tggctcggcg cagacgctgg
    50401 ctgcgatccg gacatgcttc ggctacgcgt tcgacgggcg ggagaaggaa tcgtcgatcc
    50461 cgaagctgga gacacggacg ctggacggga cgccgttcga gctgttcggc gtcgagttca
    50521 cgccgatcga ggtgcttcac ggcaaacagc gtatctacgg gttccggttc ggcgcggcgg
    50581 cgtacctgac ggaccacagc gagatccccg aggaatcctt cgagaagctt caagatcttg
    50641 acgtcctttt cctggacgcg cttcggtaca ggccgcaccc gacgcattcc accattgagc
    50701 ggtcgctcgg atacgtggag cggctggctc cggcgcggac gttcttcacg cacatctgcc
    50761 acgacctcgc ccacgaacaa gccgaacagc ggctgcctcc gaacgtgcgg ctggcctatg
    50821 acgggcttga gatcacggta caggagcccg cccgatgaag caggcgcccc gcgtgttccg
    50881 gaacctggaa gcagcggaca cattcggccc gtgcgcgctg acaatcggca actttgacgg
    50941 tgctcacgcg ggacaccggc gcatcttccg gcgggtgaca gagctggcgc gggagaacgg
    51001 ctggaaggcg tcggcgatga cgtttgaccc ccaccccacc aaggtagtcg cgccacaccg
    51061 tgcgccgaaa ctgttgacga cgcccgagca gcggtgcacc ctgatggcac aggaaggcat
    51121 cgatcaggtg ttgatcctgc cattcggacc ggagatcgcg tgtctgacgc cggaggaatt
    51181 cgcgcgacgg atcctcgtcg agcggctcgg tgcgcgggct gtgttggtgg gcgacaattt
    51241 tcgcttcggc cacggacagg ctggggatac ggcgacactt acgaaacttg gccagacgct
    51301 gggcttcgtg accgaagtga ttccggcggt ggccctgcgc gggcggacga tcagcagcag
    51361 cggaatccgc cggctgattg aggcgggcga agtagccttg gcggcgcggt tgctcgaacg
    51421 gccgcacttt ctggaaggcc cggtggtgcc gggccggggc gtcggctcta agaagacggt
    51481 gccaacccta aacctccggg tcggagcgga agtgctgccg gcgacgggcg tgtacatcac
    51541 ccggacgcaa gctctggact ccggacaatg ctggccgtcg ttgacgaacg tcggacaccg
    51601 gccgacgttc gacggcggag atctctcggt ggagactttc ctgctggagc ctcttgaggg
    51661 agtcgtgccg gcgcgcatcc gagtggaact gttgcgccgc ctgcgtgacg agcgcaaatt
    51721 ccccgacgcc gaaagcctga aggcgcagat tctgcgggac gcaagccggg cgcgcgcatt
    51781 cttccggcgt ctggggcgtt gggtgaaggg cgcaaaatag gccagaatga tagtgagagg
    51841 cacctgggaa ttgccatgaa tctatcaaca gccaaaattc agcatctgaa tgcgctcgcc
    51901 accccgaagg gaatcatcgc agcggccgcc atggaccagc gtggcagtct tcagaagtcg
    51961 atcgcctccg caaaaggcgt agacaagaag gccatcaccc ctgcgatgat gtcggagttc
    52021 aaagccgcgg tgacgaaagt gcttacgccg cacgccagcg caatcctgct cgatccggaa
    52081 ttcggccttg acgcggccgg cgtgcgggac cgcgaggcgg gtctgctgct ggcttacgag
    52141 cagagcggct acgacaacac acagccaggg cggctgccgg acctcctgcc gcacgtgtcg
    52201 gtgaagcgga tcaaggattg gggcgccgac gctgtgaaga tcctgatcta ctacacgcct
    52261 ttcgagacca cggaaatcaa cgacgtcaaa cacgcgttca ttgaaagagt aggcgccgag
    52321 tgcgaggcgc acgagatccc gttttttctg gagtttgtcg gctatgaccc ggcgggcggc
    52381 gacgaaaagg gcctggagta cgcgaaggtc aagcccgaga ttgtgaagcg cagcatggag
    52441 gagttctcca agccccagta ttccgtggac gtcctgaagg tggaggtgcc ggtcaacgcc
    52501 gagttcgtcg acggaagcgc ggtgtacacg ggccagaagg cgtactcgat ggcggaggcg
    52561 ttggagcact tccgtgaggc agccggcgtc gcgaccaagc cgttcatcta cctgagcgcg
    52621 ggcgtcagca acgcacagtt cgttgagtcg cttcggatgg caacagaagc gggtgtggac
    52681 ttctccggcg tgctgtgcgg ccgggcgacg tggaaggaag gcattccggt gtacgcacgg
    52741 cacggtgtga aggcgctcga ggactggctc gcgaaggacg gcgtgcggaa tatcacggcg
    52801 gtcaacaacg cgatcgccgg cgccacgccg tggtcccaaa agctgggcgt cgcggtgtaa
    52861 gaaagggaac tgaccaccat ttcgctgcat ttgcgaaatg gctgtctgtc tcccttgttg
    52921 cggtgtcctc gcttttgctc cgagctgaat tcacgccggg ttgccgccga cgatcggtac
    52981 ctgaatcacc gtgacggcgg gctcggcgag agcgctccgg atggcaggcg cgatttcctc
    53041 cggacggctc accttaacgc cttttgcgcc gagcgactcg gccagtttgt ccatctcgat
    53101 cggaccgagc gagctggcga tcgactcccc gaattgctcc atgtgacccg tccgcgtaat
    53161 cccccacccc tgatcgtcgg caatcacggc cacaaagtgt agcgactgcc ggacggcgca
    53221 ctcgatctcc gccacgttga acgtgaatgc tccgtctccc gtaagcagaa tcacgggccg
    53281 ttccgggaag cacagccgcg cggccattgc gcccgcgatg ccccagccga tgacgccgct
    53341 ccggccgcag gtgacccagt gagccggata gcggtcgcaa aggagctggt gcacccactg
    53401 gccgatggag ccaccgtcga tcaggaacac cgtgtcgccg gtcagcacct gcttcaaggc
    53461 atcgatgatg tggacagcgt gcatccccgc cttggcctgc tcgtcgcctt tccggcggat
    53521 gccggcgcgg aacgcctcgc ggcggccgcg ggccgcctca agccacgaag caaacggagg
    53581 aacgccgacc ttgtcgcacg caaccgagag gcggtcccag ccggcgcgcc ggtggtggac
    53641 ggccgcgttt gcggctaccg ctcccgcctg aaggaagccg acccggtagt cgcagatagc
    53701 cccggccatg atgacgcagt cggcatccgg gagcaggcta gggccgccag tggcggcgcc
    53761 gatcacgccc ataaacgtct cggcgggatg atcgatggac cccttgtccc agatgggaac
    53821 tactgtgggg atggagaagc gctcggcaaa ccggaccagc gcctcaccgc ctccggcgga
    53881 ttggacgccg ccgccggcga caagcagcgg acacgacgac gcggcgagcg cacgggcgag
    53941 cggctcggga tcttcgagcg gcggctcggc ggccatcggt gacatggtcc ggatgagtct
    54001 gagttcgtct acttcggcgt cctgaatgtc catcgggaac gtgagatgga ccggaccggg
    54061 cggcgcagcg actgccatat tgagcgcttc gtcgaggatc tggagggtac gctccggaca
    54121 gtcgatgact cgcgcgtact ttgtgaccgt gcgggccaag cccgtctgat cgaagtcctg
    54181 gaagtggccc atgcccgcgg tgcggtgtgc gcccgcgccg ctgacaagga gcatgggggt
    54241 cgagtcgaac cacgcggagc caacgcctgt gagtgcgtta atgtgggcca cgccgctcga
    54301 gacgcagcag acgccgggtt tacgggacag ccgcccctga aagaaggcca cgtaaccggc
    54361 cgtctgctca ttgcgggtgt caatgagacg aatacctgcg cggcgcgccg catggaacag
    54421 agggtccagc ccatggccgc agagcgtcgc catcgaagtg acgccccgcc tctgaagttc
    54481 ccgtacgaag taatccaccg ccgtcatgat gtgcctctcg gaaaaccccg ggactgactc
    54541 caaatttcgc tgcgtttgcg aaattggtgt ctgccccggg tttttccctc agaagttgac
    54601 gatgttggcg aaggagacag gatcgaggct ggcgccgcca acgaggccgc cgtcgatctc
    54661 gggctgcgcc atcaggccct taatgttgtc cggcttgacg ctgcctccgt agaggatgcg
    54721 aaccgcgtcc gcggcgcttt tgccgaactg cttggccgcc tgctcgcgga tgacgcggtg
    54781 ggcgtcggat gcgatttcgg gcgtcgcggt cttaccggtg ccgatggccc acacgggctc
    54841 ataggcgatc acgatcttcg cgaactgagc ggcatcgagt ccggcgatgc cgccgctgaa
    54901 ctgcgcggtg aggacgtccg ccgtcttccc cgcctcgcgc tcctccagcc gctcgccgac
    54961 gcacacgatc gggttcaagt cggcgtcgag cgaggctacg gtcttcttga gcacgctggc
    55021 gtcggtctcg ccgaagtact gccggcgctc gctatggccg atgatgaccc actgcgcgcc
    55081 gacagccgcc agcataggcc cggaaatctc gcctgtgaac gccccctcct tcacgaaata
    55141 cagattttgg ccgccgattc cgatccgcgt acccttcgcg gcgccgaccg ctgcaggtag
    55201 gttgacgaac gtcgggcaga tcactatttc acaatgtgtg gactgggcta cgagcgggtt
    55261 gaatttctcg aagaacgcga cggtctcggc cgccgttttg tacatcttcc agttacccgc
    55321 gatgattggt tttctcatac gattgcgcct aagaagctgg ttcctttctc aactttcgtt
    55381 ccgaaattct ccgccaccgt ctgtccgatg tcggacatgg tggcgcgcac acccagattc
    55441 actcccctct tcgccgccgg gccataggcc agcagcggta cgtactcacg gctgtgatcg
    55501 gtggacggcg tggtcgggtc gcagccgtgg tcggctgtga agatgaccag gtcgccctcc
    55561 tgcaaacgtc cgagcagcgt cggaatccac tcgtcgaccg cttcgagcgc cgccgcgtat
    55621 ccctcgacgt cattgcggtg cccgtacagc atgtcgaaat ctacgaggtt gacgaagacc
    55681 agaccctcgg gcgcttcatc gagggcagca agcgtcttcg ccatcccatc ggcgttgctc
    55741 gtcgtcttct cgtagtcgcc gattccccgc cccaggaaca cctcgaaaat cttgccgacg
    55801 ctatagactg gaacgtcacg ctcggcgagg cgatcgagca gcatcccctt cggcggaggc
    55861 accgcgtaat ccttccggtt ggcggtcctg acgaacgagc cggggctccc gacgaacggc
    55921 cgggcgatta cgcggcccac ctcgtacggc ccgtggagta tgtcgcgcgc cgtctcgcag
    55981 atcttgtaga gctcccagag cggaaccacg tcctcgtgcg cagcaatctg aaaaacgctg
    56041 tcggcggaag tgtacacgat cggcgaaccg gtgcgcatgt gctcctcgcc aagctgcttg
    56101 atgatctcgg tgccggaggc ggggatgttg ccgagtgcac ggcggccgat ccggcgctcg
    56161 aactcgtcga tgatcacgcg tggaaaccca tcccggaaga gcggaaatgg acggttgagg
    56221 tgcacgccgg cgagctccca gtgcccggta gtcgtgtcct tgccaggcga tgccgcggcg
    56281 caacgcccat agcagccgga gggagcgtcc gccggttcgc gaccagccag cggccggatg
    56341 ttcgccaggc cgagggcgca aaggttcggg atccggagcg gacgcatccg ggcgatgttg
    56401 ccgagcgtat cgctcccggc atcgccgtac acagccgcat cgggcatctc gccgataccg
    56461 acactatcga ggacgatgag aacgatccgc cggaagccca tcgcgctact ctcccaacgc
    56521 ctcccggatg cgttccgaga gtatctccgt gaagcggccg ggatcgaatc cgacaatacg
    56581 gctgacgatt tcgccacgcc tgttggcgat gatcgttgtg ggaatcgagc tcacgcgcag
    56641 caggcgggag aggccgtctt cgaagtagac gctcttgtcc cagccgttgc gctccaggaa
    56701 gccggccacc atgtttgggt cctcgtcggt gttgaccgaa aggaatacga ggtcgccgcg
    56761 ctcgccaaac ttcttcttga ctgcttcgta ttgtggatgc tgcactcggc agggtccgca
    56821 ccatgtcgcc cagaagtcga agacgaccac tttaccgcgc agcgacgaga gcgccaggtc
    56881 ttcgccgttc aggctgctta gggtgaactg catcggatcc tcgaggccgg cgttcgggtc
    56941 gaggcggcgg acgtcttccc gtcgtttctc gagcagggcc gcggttcggt cgtaggcctg
    57001 tagaacgaga tcgccgagcc ccgtctccga accttttagt ttgcgataca actcgcccat
    57061 atgcttgcgg tcgtcggcgc gggcggcgtc cgtgttgcgc gggtccgaga tggcgaaggc
    57121 ctcggcgaag cgttcgacgg cctccatctc cttacccgtt cgcatcagcg tgagtgcggc
    57181 ttcgcgcgcc gtttcggccg acgggtccct ttcgtagccc gccgcggccg ctgaggcagc
    57241 ctcctcgtga cgtccaagga tactgagagc gcgcgcctgg aagaccaagg cgcgtccgaa
    57301 cccacggtcg acctctccac gccagagcac ctggtcgcta cggcgcgtgg gcttctcttt
    57361 ttcaatcaag cggaccgcat cctcgtactc gcgggcgtag cggagggctc gtgtggcgcg
    57421 gtccttgtcg tcttgcgaga gcagcacccg gatcacccgt tcgagtgtct tagggtcgtc
    57481 gtcgccttgc gcgatcacgc gctcgccgta gcgcaggatg cggtcatcgt ccttcgtctc
    57541 gatggccgcc ttgacgagcg agcgttccat ctccgcgcgg cgcggggaat cgggaaattt
    57601 ctccagatgc ttctcgattg cccggatgaa ctcaaacgga ccggagcccg cttcttcgag
    57661 aagggagcgc agatggctgt cttcggcgtc cgtggggtcg ttctgcgcgg cgcagaccgc
    57721 ggccagcaat aacgcgatga agacgacggc ccgcatcact cggacttctg cttccccgac
    57781 cggatgtact cggcgatgct ggcggcgcgc tcgcgggcac ggttcatgta agcggaaccc
    57841 ttggccacgc ggtcgagcat cggcagcaga acttccgcgc cggccaagcg gttccgctcg
    57901 accgaaactt ggagattaat gagcgctcga tcgacgccga gccggtcgaa ccgcgccgtc
    57961 ttttcgaggt tgatcgaatg ctggacagtc tcggtgatcc gctcgaacca atcggccagg
    58021 agctgcgcat cggcgtcctg tgtatagttg aacttgacct cgtggttctg cccgccagcc
    58081 tcgtaacgaa aggtcttgat gcccatgttg gcgaccttaa gcccggattc gagcttgcgg
    58141 ttgaaccggt ccagcttgtc ggccagctca aaaatggatg tggtttcggc ctcgttcaat
    58201 tgaaggtcga tcgggtgtgg gtcgtctggc gcttccttgt actcggtctt gccggatttt
    58261 tcgacggtaa tcgagacaaa cggcaggctg ctctggggga aagacttgct atagaagatc
    58321 cgcggagcat cctgggccgc cagcgccgcc gtgagcagaa ggagggttgc gaacctcacc
    58381 ggctcgcctc cagccgggcc tcgtgcgcgg agtgggtcgc atggcagatg gccaccgcca
    58441 gagcgtcgga ggcgtcctcg gatggaacct gatccagacc cagaagcgag gtcaccatca
    58501 tctgcacctg ctgtttctcg gcgcggccgt agccaaccac actggtcttg atctcgagcg
    58561 gcgaatactc gcccagaggc aggctcgcct ccgccaccgc gagcagcgcg acgcctcgaa
    58621 cctgcgcaag cttgagggcg gttttcacgt tggccgcgtg gaacacctct tcgatagcga
    58681 cgacttcggg ctcgtgtacg cggatgacat cgacaaggcc ctgagatacg gcaaggaggc
    58741 gatgttccat gggatcggcc gggcgtgtcc ggatgacacc cgagacgacg acgcggtgcg
    58801 ctcgcccgtc gctctcaatc accccgtagc cggtccgttc ggtgccgcag tcgattccca
    58861 gaatccgcat ccccggtatg gccctctaac ccgcgagggc ctccatttcc ttctcgtcga
    58921 tgtcgaagtt cgagtaaatg ttctgcacgt cgtcgtgctc ttcgagcgcc tcggcgagcc
    58981 tgagcatgcc accggcgttc ttgccttcga gcttgatcgt gttcttcgga accatggcga
    59041 tctgcgcttc gacggtcggg atgccggcct tgtggatggc ttcgagaacc ttgtcgtgag
    59101 tatccggagg cgacaggatc tcccaattga cgccgtcgtt gcgaaggtcg tccgcgccgg
    59161 cgtcgagaac caggtccatc agttgctctt cggcggcctt ggactggtcg atgagaatct
    59221 ggctcttgcg ctcgaacatc caggcgacgc tgcccacttc gccgaggttc ccgccttgct
    59281 tcgagaaaat atgccgtatc tcgctgacgg tgcggttgcg gttgtcggtc acaacctgca
    59341 ccagaaccgc cgcaccgccc ggtccatacc cttcaaacat cacttcatcg agctgaccgc
    59401 cctcgagttc tcctgtcccg cgcataatcg cgcgcttgat gttgtccgcc ggcatgctga
    59461 ccgctttcgc ggcgattatc gcggtgcgga gacgtggatt ggcgtccgga tcgccgcctc
    59521 cgcgcgcggc gatggtgatt tccttgatga ctcgcgtgaa gaccttgcct cgctttgcgt
    59581 cagcggcggc cttcttgtgc ttgattgtgg cccacttgga atgacccgac ataagaaacc
    59641 tctcggaagg aaaaaacgcg ttcaagaacc caaaagagag gataacacag tcaatccgca
    59701 acggttatcg aaaatagggc aagtaggccg gaacccggcc gcggtagcgg tcgaaggcct
    59761 cgccgaagcg tcgggcaagc aggccgtcct cggtccggac gcggatctcc gtcccggcga
    59821 taaagatggc aagccccgcc agtgcggcct cccagcggga aaccaccatc gcgttcgaga
    59881 ccagcaaacc cagcatcgag agatagattg ggtgtctcac ggccgcgtat gggccggtgg
    59941 ccacgagctg gtggccaggt cccacgacgg cttgcaggcg gaactcacgc ccgaggtgaa
    60001 gcagagccca catgaagaag gccactgacg cgggagccag cagcacgccg atgatgacca
    60061 gcagcagtgg ctcatgcccg gctccgaacc gacggaacag ccagacaaac acataggcca
    60121 gcgtctcgag caccagtccg tagagggacg tggacgaccg cagttctggg ccgtctgcgg
    60181 tgaccggacg gctacgacgt tcccactccg cccgaatgta gaggaagaat gcgccgaacc
    60241 cgaagaccag ccaggagatt cccaggatga tgacagccac acctctcgtc ctcttcagcg
    60301 gtattatccc agtagctgcc gcaccaccgc cagcactctc tccgcccgaa gaggcttctg
    60361 aaggacggcg tgagcaccga ctgcctgagc aacgtggaga aactggcccc cgaacgcccc
    60421 cgaaacggca atgatcttca cttccggccg ttccttatga aggctgccga tcatctcgat
    60481 gccctcgcac tcgggcatca cgatgtcggt gattacgagg tccgcggaac ggtgggcaag
    60541 ctgttccata gccgcccgtc cgtttccggc ctcgaacacg tcgtatccgg ccgcctccag
    60601 aatccgccgc agcagacgcc ggatttccgc ttcgtcgtcg accacgagga cggtggcgct
    60661 gttttgcgcc cggtccagca actcccgcac cttggaggcc agatcggatc tggtgaaggg
    60721 cttctgcaga aatgcactgt ccggcgggaa cggaagtcgc cggctttcgg gagcgtcggt
    60781 gtatcccgag atcagcaaca ctttggtctg cgggcgaacg gccgtaatcc gctgagccag
    60841 ttcgcgcccg tccatgccgg gcatgacgag gtcggtgacc atcagatcga tgactcccgg
    60901 atactgcgcc gcggcggtta gcgcctcatc cccgttgctc gccacaagga ccttgtagcc
    60961 acaggattcg aggatccggg cagccagctc tcgcacatcc tgttcgtcct cgacgagcag
    61021 cacggtctcg ctccctcctg gagactctcc tcccagcttg acgccctggt gctcgtccgc
    61081 tgcatcctcg gcccgtggaa ggtagaccga gaatgtcgcg ccgagacccg gctggctgtc
    61141 cacccggatg gagcccccgc tctgcttgac gatgccgtac acggtcggca atcccagtcc
    61201 ggttcccttc ccctgttcct tggtggtgaa gaaaggctcg aaaatgtgct ccaggatctt
    61261 cttgctcatg ccggttccgg tgtcggcgac cgagaccatc acatacggcc ccggctgaac
    61321 cagtgggtcc tcgagcaggt ggtggctgtg cagttcaaca ttcttcgttg caaccgtgag
    61381 ccgccctccg cccggcattg catcgcgggc attgaccgcc aggttcacga tcacttgctg
    61441 gatttgtccc gggtcagctt tgatcttgcc gagattcgga tcgagtaccg tcgtcagctc
    61501 gatatgctcg ccgatgagcc ggcgaagcat cttctccacg tcagcgacgg ccgcgttgag
    61561 gtcgatcagt ttcggcttca gcaactggcg ccggctgaag gcaaggagct gttgcgtgag
    61621 tgaggcggcc ctatcgcccg ctttgcggat ctcttcgagt tcctggctta gcggatcgag
    61681 cggattaagc cggctcatcg ccagctcggt gaaaccgtta atgaccgtga gcagattgtt
    61741 gaagtcgtgc gccacaccgc cggcgagctg cccgaccgcc tccaacttct gcgactggcg
    61801 caactgctcc tgaatacgcg tgcgttcctc ctcggtgcgc tgccgctcga tggccatggt
    61861 gtaaacggcg gcgagacgtt tgaggagatc gagatcgtcg tctgtgtaat cgcggccgct
    61921 atttgccacc gcaatctcgc cgaccgcctc gtctcccagc acacagggga cggccagaaa
    61981 acgccggatc gggagatgcc cggcgggtac gcccgtggac cgcgcgtcgc tcggcacgtc
    62041 gtttgtcaac acgggcagct tgttcttcag aacccagccc cacaggcccc cgaagcggcc
    62101 gaagatcaca ctcctgtcct cgatcctgca atccgcccat acgtccccgc tcagagtcgg
    62161 ggcgacgaac gtgcgatccc gcggatttac gtagcccacg aagccgtgcg ggctatccgt
    62221 gtgcagttgt gcatgcctga ggatcgagct ggagatctcc tcgtgcgagg ccagtgacag
    62281 caggtgcgcc gacatctcgg cgatcgactc attcatccgc agttgccgcg agatctcctc
    62341 ttccattcgc ttgcgctcgg tgatgtcctc cttgacggcg aggtagtggg tgacggcgcc
    62401 gctggcggtc ttgatgggcg agattgacgc gttctcccag aacagctcgc cgttcttctt
    62461 cttgtttcgg aactcgccgc gccattcacg gccggcgcgt aacgtgttcc agagttcgcg
    62521 atggacggct ggcggggttt cgccggactg cagaagtcgc ggattgcggc cgagcacctc
    62581 cgacggcgtg tacccggtga gctcggtgaa cttcggattg acgtactcga tgtccgccgc
    62641 cgtgttggtg atcacgatcg aagctgggct ttgctcgacc gccaccgaaa gcttgcgaag
    62701 ctggtcctcc gcctccttct gcgcggtgat gtcgatcacg atgccaaagc catattgcgc
    62761 ccggccgacc gcgtcccgag ccacttgcgc cacgagccgc gcccagacaa ttcgtccatc
    62821 cttgcggata tagcgtttgg tgagcgtcgc tccggattct gccccgtcca cggtcgtcgc
    62881 gcggcgaaag atctccagct cggcggcgat gtcctcggga tgagtgaggt cggtgaacct
    62941 catccggcga agttcgtcca agctgtagcc gaccagcgcg gcgaaggcgg ggttgcagtg
    63001 taagaatcgc ccggtggtat ccacgacagc gacaccgatc ccgatcgatt cgaagatcac
    63061 gggcaactgt gattcgctga atcgaagcgc gtcagccagg tccgagtccg gttgtggcga
    63121 cggttcaggc ttcacaatcc tgttaagacc tctgcgccca aagacttatt ggaattccac
    63181 agcgggatcc ccttgggtgc cggcaacggg caggctccac ccaaagtata tccgatcggc
    63241 gaataccggc cttgagaaca gtccaggaac ctcatcggcg aaagaggtta cgggccagga
    63301 agaggacgtt ggcagggcgc tcggcgaggc ggcgcatgaa gtacgggtac cacgcgccgc
    63361 cgtagggaac gtaaagacga agccggtagc cggaggcgac gatgcggcgc tggaggtcgc
    63421 ggcggatgcc gtagagcatc tgaaactcga agcggtccgg cgcgatgccc cgctcgcgga
    63481 caaagcgcag cgcctcgtta acgagcgttt cgtcgtgcgt cgcgagcgcc ggatagctgc
    63541 cgtgatcgag cagctctttc atcaggtgac catagctcgt atcaacctcg cgctttccgg
    63601 cataggccac tgacgacggt tccttgtaag cgcccttgca tagccgcacg gggacgcctt
    63661 tttcgttcag catccggacg tcggacgcgc tgcgatagag atacgcctgg attacgccgc
    63721 ggacgcgccg gaaacgctcg tgcatggcgg caacaatgcg gagagtgcgg tcggtgtact
    63781 cgtgcgactc catgtcgatc tcgacaaaac cgccgattcc ggcggcggct gagaccaaac
    63841 gctcgacgtt ctcgcggcag aactcctcgg agagatcgag gccgaactgg gtcaacttga
    63901 tcgagatgct ggcgtcgagc tggtgctccg cgatgcgctg catggattcg agataggcag
    63961 cggcggcggc gagcgctgcc tcctcagagg tgacgttctc gccgaggtga tcgagcgtca
    64021 cgaggattcc ctcgctgttc agcttgcgac agacgccgag gccctcatca agagtttgcc
    64081 cggcgacgaa ccgtttggtg aggagacctg ccgctggcga cgtctccatc catcgcctta
    64141 agtgctgccg gcgggagagg aatagcagca ggctccgcag caccatggga gcatggtatc
    64201 aggaaggctc gctaacaacg gcttcccggg gcatcacctc gacgctagcg ggggccagag
    64261 gttcggtgcc gtttactgca ccaagctcgc gtacgcggcg aagggagggc aacagacggc
    64321 tctcccaaga ggcgatggct cggttgtatg cttcaatagc gcgctcgagg tggcgcccgg
    64381 tgtcgctgta gtggccatgg atgatctgga tacggtcgga gacctcgacc gcaacctggc
    64441 ggatctgttc ggcgttttcc gcaagacgcc gctcgcgcca tccgtagctg attcccttga
    64501 gcacggagat aagtgtggtg ggagtggcga ggaggacttt gcggtccaca gcgtcctcga
    64561 tcagcgttgg gttgtactcc agcgcggcgc tgaagaagtg gtcgccggga atgaagagga
    64621 ccaccatctc aggggcgggc tggaactggc tccagtactg cttgctgctg agctgatcga
    64681 tgtgtttacg gacttgctgt ccgtggcggt tcagcgcatc gcggcgccct tcttcgtcat
    64741 cggaagagac ggcatcgagg taggccgcga ggggaacttt tgcgtcgacg acgagcgacc
    64801 ggccgccagg caatttgacg atcatgtcgg ggcggatgcg cccatcctcg gcatcgtggg
    64861 tctcctgctc gacaaagtcg cagtactcga ccattccggc gagctcggca acgcgccgca
    64921 gtgtaatttc accccagcgc ccccgcacct gcgggttgcg gagcgcgttg gcgagcgtga
    64981 cggtttcctt gccgaggctc tggatctgtt cgcggagact gccgaacatc tgctggcgtt
    65041 ccagttccag ttccttcact tggccctcga gccggctgag cgcgccggtc aggggctgga
    65101 tgacgccttc aatggcagtc tggcgctgcg cgaggtcgcc cgacatctcg gcacggacgg
    65161 tttcgagaga gctgcgggca gtatccagga acgcgccctg gctggaacgg agcgcggcgt
    65221 cggcaagcgc ctgaaacttc tcgccaatct tgtcggcggc ctggtcggca gcggtgagcc
    65281 gggcttcggc gacatttttc gccgcctcca cccacgcgag cttgccgcgc atccaaagcg
    65341 cgaggacgag cccgccgatg gtggcgccga ccaccagccc catgaaaaac atcaaggaaa
    65401 ccgtcgactc cataacttct cccttccctt agaatttaca ccggattctc gacaatgaga
    65461 acactttttt cgaagatgtt ttggtaaatt cgcacccgga atatttatgc ttgggcgtgc
    65521 tgcgagcggg ccggcggccg gtagcgcacg tcaaggtgag gcgtctcgag gcgcgcgtgt
    65581 ccctgaactc gcgacgtcag gcaactctcg agtacgccgc tgaccagcag cgtgcgctcg
    65641 actgggtagg gcgcctcgcc cgtctcgaac atctcctcga tctttcccac caggcatgcc
    65701 gagtacgtga cgttgggcac gggcgggaga aagaattggg tcgaaatcgt gccgattccc
    65761 ttcacccggg cggcgaaatt gtagtccttg atcgccccat tcagcatcag cagcgtggtc
    65821 cggaggccgt cccggtgctc aatgaagtaa gcagccgggt tcttcacgag ttgcggtaaa
    65881 actccgctcc ccacgaggtc ctgggggcgg ccgtctgtca gtgtgagtcc ctgcggggtg
    65941 tcgctgcggg acagtgcggc ggtgagcaac tcgcgcgacc agcggccccg ctctcccgct
    66001 tcccaaacag catcaccctc gactaactgg accgccttca cgccggtctc ccctcccttt
    66061 cgccgttcaa tcatgcattg catggcttcg agcgcgtggt agtccatcgg atcggagccg
    66121 cccactccgg tcatcaacgc gccttcgatc tcgcagccaa gcggaagctc gacgtcggga
    66181 agccgccatg tgacgggaag cgacgagccc gcgagcatcg ggaacttgag gcgtcttgag
    66241 gcctccacca tccacttcgc cttctcgaag ctccaagaaa ggtgcttgtc gttgtaaaca
    66301 gggaccgtcc gaccgtcctt ttcgaaaacc tcgacgcatt cgcggaagaa ctcgtagcgg
    66361 gggtagagga cctggccctt ttcattgcgc gggtagtctc cgtgctcgcc gatcacgagg
    66421 accgcgtcca cggccaactt gtcgccgccg caacgtagtg cttcggcgat ggtcgggtaa
    66481 acacggaagc cgtactcccg cgcccgggtt tcgctgaggt ctccgtcggg tttctgatcg
    66541 acatagagcg atgcgacctt catccttgat cggtgccagc ggccgttcag ggggtagccc
    66601 accaggaacc ggtccgccat gtgctgcgcg tgcgagagat agcgatagat cgtggtgatg
    66661 actgcgatgc gcttgctgtt ttcctgggcc ggcatggctt aagtcctcca gaacgtggat
    66721 tcgcgcgggg gggcgtagcg gatggcaaga tgcggcgtct tgatgcgcgc ctgcttctgg
    66781 tagaggcttt ccacgcccgc ggcggtgaga ccggtggtca gaagcgtccg ctcgaccgga
    66841 tagggtgggc ttccgctcag gaacatccgc tcgatgttgt tgaccagcgg ggtgaagaac
    66901 gtggccgtgc tggtccgccg aggcggcata gggaggtaca tctgggtgga gaacgggtcc
    66961 gcgtgcccgt caatccgggc agcgaagttg aagtcctcga ccagcccgtt catcagcagc
    67021 atggtgcaga cgagcccgtc ttcgtgctca tagcggtagg caatgggatt cttaacgagg
    67081 cgcgcgaggt cctccatcga cgggtaaatg tggttgtagc cctcacgggc cggagtgagg
    67141 gtgtggctgc ggcacaggca ggcctcgaac aggtcagggg accagacctc ttccttgtgg
    67201 gcgtgccaga actcttcgcc tcggaaggcc tggagccatt tgataccggt ttcgccgccg
    67261 gcgcggcgtt caaccatgca ctgaagggtt tcgagcgcgt ggaagtcgta gctgtcgggg
    67321 ccgccatagg cgacgcacat cgcttcgcgg acacgggcgc cgagaggcat ttcgagcgga
    67381 ggtgtgcgcc acgtaacggg caggctcgag ccggccatga gggggaatcc catagagcgg
    67441 gcggtgtcgt acatctcctt cgcgtagtcc caattccagg agaggtgctt gtcgttgaac
    67501 acgggaaccg agcggccgct cgcgcggaac accttaacga tctcctggaa gaattcgtag
    67561 cgcgggtagc gcgtttgacc cttctcagtc ctgggatacc ggccgtgttc gccgatgagc
    67621 agaacgccgt ccacggtgag gctgctcccg cccagagtga gggcttcggc gatgctggcg
    67681 cagacccgca tcgacggatg gcgggaggcc cgctcgcggc tgaggtcgtc cgagccgact
    67741 tggtcgacaa aaagagacac caggtccatg ggtgggcgat ggtgcgcgct gttccagccg
    67801 tagccgtcga ggaagcggtc gacgatgtgc tgggcgtgtg agtgtgtgtg gtagatcgtc
    67861 gtaagggcgg cgatcttcgg cttgacgggc cggcgctgcg ccctcgcggc gaggggcgcc
    67921 gaccccgccg ccttgaggag gtctcttcgg gaaattcgca tgacttccac ctgacgttcg
    67981 cgagccatct tatcacctgg aaaaggcggt cggacgctgg cgaattgcgt tgctgagggc
    68041 catcagcttt tcggcttcgg ctcgggcgat ctgcgcgagg gggcgaagaa gctcatctcg
    68101 gagacggact ggttgttgag ggctgcggcc tgctcgagga tgttgcgagc gtagtcggcc
    68161 acgaggcgca cgccctcctc gtagcccatg ttggggttcc ggatgccgcc gttgtacgcg
    68221 ccgacggccc gggcgacatc gccgttgaac cggtcgatga ggtgccggaa gaggagaccg
    68281 gcgtacgtgg tgagcaggcc ggagtcgatc tcatcgaggt tcaacttgcg cggtggccgg
    68341 agagagaggg ggagccggcc gtaatcgcgg gtgtctttga gaaagaggcg gtggacataa
    68401 cgcaacgtct cgcgagtaat ctgccacggc ccggtggcgt aattcagcac gagcacacgg
    68461 ccgcggcgcc ggcgcagaat gatgtcgcct ttttgagcgc ggcgtttcca gacgacgtgc
    68521 cgtagatcgc cgcgaatgtc catatagttg ttctccatcg cgccaatacc aaggaagaac
    68581 tcgagcggca gttcatagaa ctcggcgaca gtgatgatgt ttgccgccat cccacgggct
    68641 tcgtcgggcg aaagcggttc cggcgcaggc tcgtctcctc tgcgaatgcg cgcgtcagtc
    68701 gcggacttga agtgaatgaa acgtgccatc aggttgagca gatgtccgcg tccgatgccc
    68761 tcgaggtgcc ggcgaaccgg gagggagtag gccgcttcga aagtgcgcgt ctgctgttgc
    68821 cagtccgcaa gcacgctccg cgcaacccat gcgagttcga aacctgcgat gtagcctgac
    68881 gccacgaggc cggacgcgga actggcggcc gtgattgcat cccggttcca gggatagagg
    68941 tagaaccggt aaacgggccg tccgccggtt tcccgcgtgg tcaataggac ctgcatcctg
    69001 ctcgattcgc gacgggagcg caggtaatcg tagcggaggt aggcgagcag ttcgtcgctg
    69061 gattcgcccg ctaggacaac gaattccttg tcggccgaga ccaccgcgga cagtcccgcc
    69121 gcgaaagacc atatcggcgt tcctgaaggc gggggtagcg ccagttcacg gcgttcgagg
    69181 cgctgccagc agtatccgcc catcaggccc agcgccaggg atgcgagcaa aacccagcta
    69241 cagaaggcct tccgtgtcat ggcgcacctc tattatcgcg tgcgggcggc cgggccaagc
    69301 agttcaaatt cgcgtccact ccgtgcgtgc ggcggcgagt aggcggccat cgccggtgtg
    69361 gagggcgtgc cggtaggagg gcgcatctgc agtcgggaac tcctcggcgg cggagatcac
    69421 ttcgtcgtcg agaaaggcct cactcacaaa ccggacgtcg agcgccgata gcctcccatc
    69481 gcggcgaagc gcgagcggca cggtctcgat cgcccaatcc acatagcaga cattgttggc
    69541 gtggccgttg gcgtcaaggt cgtgccagcg gacgcggttc cgttcctcga ccgacggctg
    69601 ctccgggggg atcgcgccca ttgcgtctac ccgatccgcg cccgcgcgct ccggatggcg
    69661 gaagcgcagg attgactccg gaaggcgcac cggccgccga gtcgccgcat ccagcaacaa
    69721 ccagagactc gaagcctcgc cgaatgtccg gccggcagcg tccaggagca cgaagtctcg
    69781 atacgcacgc acgccgccga gccggcgcgt gccccaggtc tcgaccgcga cctcgtcgcc
    69841 aacgcggggg agccccgtga gcctcagcga gagatgcgtc agcacccaaa gccggccggc
    69901 ggcccgcagg tcgtcgagcg agaggccgta cgcacggcag tggatgtcgg cggcttcctg
    69961 gagataccgg caaatcgccg gcgccgacgc gcgcccgccg ggctcggctt catagaagcg
    70021 gacgcgatac gtctcacgcc agacgaggtt cattccggaa gtgtaacagg ggtggaaagg
    70081 cggcctatgc ttccgcgagt ccggcgccgc ggatcactcc ccggagttgc gccagcgcct
    70141 gcgtatagtt cttcgagccg acgtactcga gaaacaatgg cgcaccgggc ttgcgctcag
    70201 tcgctagagt gacgaacttc cggtagtcga ggtcaccctc gccggcgggc acgccgcggg
    70261 tcacatggag cttgcgatcc ttcgcgtgga agcagtcgat ccacggatcg agttgatcga
    70321 acatcgcctc gatgctgtcc acttcaagca ggttggccgg gtcgagcagc gcccggatgc
    70381 ggggcgagcc gacttcctcg aggaacagcc gggtgcgccg cgcgctggcg aggaagccct
    70441 ggaaatacgg ctccagcagc acggtggcgt cgtgcgaatc cgcgatgcgc gccagttcct
    70501 ttcccgtgac cagcatctgg cgccagactt cctcctgcca gtggtgcggg accggaggtt
    70561 cgggttccgt gccgcgccag tgtccggctt cgctgatgaa tttgcgcacg cccatgtggc
    70621 cgcccaccgt catcatcgcg tcgaagtacg cgaggttcgc cttgcgctcc gcctcctccg
    70681 ggtgaatcag gttcgtgtag acgccaatgc tgtggatgga gatgccggcc gagcggtaaa
    70741 tgcctgcgat ttcagtggcc cgcgccgccg tgaggccggc aagatcgctg cggctgttgt
    70801 acttccagta gttgctgtcg gtctgggtga ggaagagctg gataactcgg atgccctgcc
    70861 ctttcagttc ctgagccatt cgggcgttgg tgtagtcgcg aaactcggta gtggccatac
    70921 cgatggtcac cgtcttcccg gccttcgcgg agacggtcac cggcagagcc acggccgcga
    70981 ggagtgcgtc acgtcgggtg atcgagtgcg gcatctatat ctccagcttc cagggagcgc
    71041 ggtacggtct caggaccagt gcgtctgcct gcggatcgcc gatcacgcgg ttcttctcac
    71101 cgtcccaacg gatgctgcgg ccggtttccc acgagatgtt gagcaaatgg ccgacagcgg
    71161 tggcgtagtg acaggtctca acggtcgctt ccggctgcct gcgcgcgcgg atgttgttca
    71221 ggaagacctc aaagtggtag tcgtgatcgt ctttcggagg tacggtctgc tcagggttga
    71281 ccagcccggt tgtggggacg gcccgctgcc ccttcttgac ctcgggaacg atcgagtatc
    71341 ccgcgcggtc gagaaccatc gacgcctcac tgcccataaa gcacttgccg tgggcacgcg
    71401 tctcgcggcg gcagcccgta cgcatggtgt agttgaggac gaagccgcgc tttgacactg
    71461 ggccggggcc gaattcgaga gttccagtga aggtgttggg gtactcgtga ttgtcctcga
    71521 aggcgaagtt gccgccggag gcgaaggccg tcttcggcga cttcactccc atggcccaga
    71581 gcacgacatc gaagtgatgc acgccccacg cgacctggtg gccggcgccg gagactttga
    71641 accagtcgta cccgtagttg tagtagacgt tcggattgta agcgcgccag ggtgaggggc
    71701 cgacatagaa atcccagtcg agctccggcg gcgggtcgca atcggccggc gagcccgagc
    71761 ccggccagta gttctcgcaa tcccagacgc gcacctcgga gatctcgccg agcttgccgg
    71821 attgaatgag ttcgactgcc tcgcgatagt gctgctggct gcgctgctgc gtgccggtct
    71881 ggacgatgcg gccgtacttc tttgccgcct cgatggcgac agcgccttcc ccgatggagt
    71941 tgccgagagg tttttcgaga tagacgtctt tgcccgcctg gcaggcggcg atcatcggca
    72001 gcacgtgcca gtgctggttg gtggcgatga tcaccgcgtc gatggttttg tcctcgagca
    72061 gcttccggta gtcgttgtaa gcagcgacgc ggtcgccgcc agcccgctgg cggccgtcgg
    72121 cgaggtgttt ggagttcacg tcgcagacgg cgacgaaccg cgtgccagga agcccgagaa
    72181 agcgcgggat gatcagcgtc ctgccgcgcg cgccgcagcc gatcagggca aggttgatcg
    72241 tgtcgttcgg accgggcgct gcaaccgcgc cggcaccgta agtgcggggt atagcaatgc
    72301 cagcggcggc ggaggcgcca aggaattctc ttcgtgtcga tttgccggtc atttcgggtg
    72361 atattatcac cccggcgtgc ccgctgtgac aaaaatatga caaaaggaac ggcccaggcc
    72421 ggcgtataat ttagcttaat tgccttctaa tccgtatttc cgggctgaaa gtccgttcta
    72481 ttgatccgcg ggactggttc aaggtgactt ctattcaaca tcccaaaagc aagcacgcgc
    72541 gagttctgtt gtcgagcgtt ttcgggcctt acgcgcagga agacgagttc ggcagccgca
    72601 gcatcaaccc catggagctg taccacaacc aggtgacgcg cgcgcagggc gtgttttcgc
    72661 tgcgcatgtt ccaccgctcc tgggggatca tgatgatcca acagaacatt tccaacccat
    72721 cgacggtgct cgattttccg aagcgcgacg acttcatccg cgagatccgg aaccaccgtt
    72781 acgacatcat cggcatttcc tcgatcatcg tgaatgtcgg taaggtcgcg gagatgtgcc
    72841 ggctggcgcg ggagcactct cccggatcga cgatcatcgt cggtggtcac gtttccgcca
    72901 ttccaggcat cgagaagatg gtggacgccg attacatcgt gcggggcgaa ggtatcgcgt
    72961 ggatgcggag ctatctcggc caggacgccg gcgcaccgat cgtgcatccg gagatcgttt
    73021 cgggcttcga catgcgcgtg atggggttca aaatcccgaa caaaatgtcc gataccgccg
    73081 ccacgatcat cccgtcggtg ggctgcccga tgggctgcaa cttctgtacg acgtcggcgt
    73141 tcttcggcgg taaaggcaag tcagtcgact tctttgagac cggcgacgag cttttcgaag
    73201 taatgagccg gatggaggag aggctgaatg tgcgcagctt cttcgtgatg gacgagaact
    73261 tcctgctcaa ccgcccgcgt gccatgcggc tgctcgagcg gatgaaggag gcggggaaga
    73321 gctgggcatt ttacgttttc tcatcggcca acgcgatccg caagtactcc atggaggaac
    73381 tggtccagct cggcatttcg tgggtctgga tggggcttga gtcggtgaag tcgagctatg
    73441 cgaagctcaa ggacacggac acagtcgcgc tgacggcaga gctgcggaaa cacgggatca
    73501 agctgctcgg gtcaaccatc gtcgggttgg agcaccacac gccggaaaac atccgcgagg
    73561 aaatcgagcg cgcggtcgcg cacgacaccg atttccacca gttcatgctc tacacgccgg
    73621 tgccgggcac gcccctgtac caccagatga aacaggaagg gcgcatgctc gaggacgtgg
    73681 atctggccga catccacggg cagtggaagt tcaatttcaa gcatgcggcc atttcccggg
    73741 acgagtccaa gcacttcctc gacctggcct tctggcggga tttcgagcgc aacggcccga
    73801 gcctgtaccg catctgccgc acgacgttcg aaggctggcg gcgttacaag aaccaccagg
    73861 accagcgcgt gcgcgagcgc ttccagtggg aggtgcggac gctgcggcgc gcctactccg
    73921 gcatgttgtg ggcgatggaa cggcaactgc gcgattccaa cgaagccgtc agccggcggg
    73981 tacgcgagct acggcatgag ctggagcggg agttcagcgg gttttcgaag ctggcgaccg
    74041 gcgtgcttgg tccgatgttc ctgtggatga gcaagcgcga gcagaagcgg ctggaagccg
    74101 gacagaccta cgaaccgcag gtcttcatcg agcgccgcaa ctgggaagcg gcagtgtagc
    74161 ggcatgctgc gccgaatcct cgcgccggcc ttcgcgctgt tgacgctcgg ccagggattc
    74221 gcgattgcgc agcaggcggt cgacattgac atctggcgga cggcgggacc gttttcagcc
    74281 ggactcgcca ggccggtcaa ggtcgtggat tccgacggga gggacgccag cccgaacgtc
    74341 tcgcgcgacg gctccaggat cgtcttttcc tcaacgcgtt cgggcacgtg ggaggtctgg
    74401 acgtgccgta gcgacgggag tggcgtcttc caggtcaccc ggctcggcga cactccggtc
    74461 ggcagcaccc ggtggtcgcc cgacggcaag aggatcgcct tcgacggcca gaaaccgggc
    74521 cactacgacc tcttcgtcgt gaacgcggac ggcagcgggc tgcggcagct cacaacggag
    74581 agttccactg acgtgaaacc gagttggtcg aaggatggcc gctggatcta ccacacgtcc
    74641 aaccgcaccg gcgagtacca ggtctggaag atcttgtcgg ccgggggaga ggcggtccag
    74701 gtcacgcggc gcgggggcta cacgccattc gagacgatgg acgggaagtt cgtctactac
    74761 gcgaaagggt tgaacgagca cagcctgtgg cgcgtgccgg cgagcggagg ggaagagtcg
    74821 ttcgtactgg acaacgtggt gagtaaccgg tgggtgatgc tgaagaacgg gatcgccatc
    74881 ctgaacatgg acgcgcagcc tctgccggtg atcgagttct acgacttcgc gaccggccgg
    74941 cgccgcgcac tcgacgtgct acccaaagaa ggaaacatct acggcggcgg cactgccatc
    75001 gctgcgccac ccggcggagc gtggctgctg ttgtgcgcga tcgaacctgc caagcgtcca
    75061 gagtgaggcg gcgaccgaag ccgccctttt ccggtaagct ctacccatgg gtcgattcct
    75121 tctgaccgct tccctgctct tgtgcgcgag cggggcctat tcgggggaac tccaggacga
    75181 tttgaaagcc cggcgggcgc ggctgatgga gaagctcggc ccgaagtcga tgctgatcct
    75241 tcacagcgca tcggtgaaga actactcgtg ggacgtcgac cacgagtacc ggcaggacag
    75301 caacttctat tacctcacag gtgtcgagca ggaagagtct gtgctggtga tgatgccggg
    75361 gaacagcaac cggcgcgagg tcctgttcat ccgcgagaaa gacccgaagc gcgagcactg
    75421 gcaggggcgt tcgcttagcc aggcggaggc ggcggagcaa agcggcatcg agaccgtcta
    75481 ctggcttggc gagttcgagc ggttcgtcga gatgatcctg tcgggggaac cctaccgccc
    75541 ggagaaagcg ccgcggctgg atcggtctcc ggacttcgac acgttcctcc acgctctggc
    75601 agggggagag gcggagctgg ctgtggtcct cgaccagact ccgacaatca aagggccggt
    75661 gaatccggcg ctcgctttcg cccacgacct gcgcgaccgg ttcctcggat ttcgagtcgt
    75721 cgatgcgacg cggcacgtcc acgccttgcg gcaggtgaag tctccgtacg agcgcaaggt
    75781 gctcgaacag agcgcggcga tttcggggga agcccaaagg gccgggatgc gggcggcgcg
    75841 gccgggcgcc tacgagtacg aggtgaaagc agccatcgag caggtttacc gcggccgtgg
    75901 cgccttcggt tggagctatc cgccgatcac aggcagcggc ccgaacgcca atattctcca
    75961 ctacgccaaa gcggaccgcc ggatggatgc gggcgagctg atgctggtgg acgcagcggc
    76021 caacttccag tactacaccg tggacatcac gcgcacctac ccggtgaatg gccggtttac
    76081 cgaagcgcag aaggacatct actcgattgt cctgcgggcc caggaggagg cagcgaagtt
    76141 cgccaggcca ggcgtcctgt tacgcgacgt acaccacaaa accgtcgatg tgatcaagga
    76201 cggcttgcag aggctcggcc tcctgaccga catcagcggg gaccagtact cggtctggta
    76261 tacccacggc tcgacgcact acatcggcat agacgtgcac gacgtcggcg actaccagga
    76321 gccgctggcc ccaggacacg cctttgtgat tgaaccgggc gtctatattc gcgaggatgc
    76381 tttcgagagc ctggacaaga cgccggagaa cgaagagttc accaaaaaag tgaagccggc
    76441 tttcgaaaag tacaaaaaca tcggcatccg catcgaggac tcgttcttga tgaccgagaa
    76501 cggcttagtc aacctgtcgg ccaaagcgcc caggacaatc gaggaggtcg agggcttcaa
    76561 aaaggggcgg taggcgcagg cgcccggtgt ataatcggcg cagccgggga ggggccatgc
    76621 cgaagaagct ggtcaggcgg aagccggaaa agcaaaaaac gaagaagcca cgggtcaaac
    76681 gccgtgtgcc gccacctgag acgcctccgg acatcgaggc tcctcccgtt ccctaccagt
    76741 ggatcaccgc gacgctgcgt acgagtacgg ggctcttctg tcctatcacc agctctcccc
    76801 tgttgcggcg gcgggcgatg gagttcgcct attcggttcg caaccgcgcc cactgggccg
    76861 aagtcgaatc ggcgcgcgag cagttaaagc agcggggcag tcaggtcctc aaggaattcg
    76921 gtctcagcga caccgatctc cggcagatcg ccgaagcggg tatcgtcgag gtgaagattc
    76981 cgtatccggc ggaatcgatc ggatgggaag tccggatcct gccctgggag tatctgctca
    77041 gcgccgccac caaacctttt cgtggtgacc gggcgctgca tgtggtccgc tatctcgaaa
    77101 gaacaacggc ggcaggcccg gccgcaaaga cgcccgcatc cctcctgttc gtcgaaaccg
    77161 cgcccggccc gctcagcgaa gactactcgt tcgagttcga gcgcaagctg gtagaggcca
    77221 acctcgaact cgatgccgat catctgatca acccgaccct cgatgcgctc aagaacagaa
    77281 tcgggcaccc cacaccggac gtgatccatc tgacgggctt cgatacccac cagggcgtgg
    77341 acttgctcga ctggcacttg aagcagccga aggacgggtg gaaggacggg atgttcttcc
    77401 gagggcctga cgggctgccg cagatcgtcg aggccatccc gatgtcggag gccttcgaag
    77461 cagtgaagga ccacccgccc gcgctcgtgg ctttcaactg ttacaactct gcgtcgaggc
    77521 tggcagccct ggcggtgggt gcgggtgcag gcgcggccat cggcttccag gacgatatcg
    77581 acgattttct cgccgagctg ttctacacaa acttctatcg cgcgtggaag ctttcgggtt
    77641 ggaacatcct ccaggccttc aagtgggcgc gaaactcgct ccggggccaa agctccaagc
    77701 ttcgcggcac cggcgtgata ctctggagcg ccacgccgtt gctcgggctc gaagaggcgc
    77761 ccgacgagct ggagaagcgc ttccgcgacg accatcggcg cgccgtccag gttccgcccc
    77821 ctgcccaggg cggggtccgc gagatcgtta ccgtcgaggt ggaaccgctc gataagctga
    77881 actactcgct gcttcacaat gaccgtccgc tgttcgacac cttccagatc cgcaagtacc
    77941 ctcccggtcg gctgagcggc gtgagcgttg aggtcatgtt acacgccggt cccgagagcc
    78001 tgcgctacca gagttccttc gacctcgagg acccgatcaa ggacctgaag gatctggtga
    78061 aggttccact tacttcggag ctggcccgtt cgattcggga gagcgtgcag acctgtgtgt
    78121 ttgtcaacgt gtcctgcgga ggccaggacg tcttccgcga cacgtatccg gtaacccttc
    78181 tgccgatcga cgaatggcag gatgacgatc tgaatcgcgt atggctgccg tcgtttgtga
    78241 tgccgcggga cccggtggta gcgcgaatca tcgacgcggg acagcggtac ctgatgtcgc
    78301 tggccgacga tgccggcgcc ggcttcgacg ggtaccagag cgtcgatccg aagtccgacg
    78361 acccgagtga aggcgtcgac ctccaggtgc gcgcgctgtg gtcggcgctg ctgaacgagt
    78421 accgtctggg ctacatcaat ccgcctccga ccttcaccga ctccgcacaa cggctccgca
    78481 cgcctgtgga catcgtcgag agccgccacg gcacctgcat cgacctggcc ctgttgctcg
    78541 cctcctgcct ggagtacgtg gacatttacc cggtggtctt ccttttggaa ggccacgcct
    78601 tccccggcta ctggcgcagc gacagtgcgc acgagaagtt ctgggaacgc ttctatgtca
    78661 agacgttcga acagaccgag gagcctccgg acgaacggat ggtcccgaac gcggccggag
    78721 cggagccgtg gatgctcggc gccgagggat atgccagcgt ggtcgagctc gtccgcagcg
    78781 gcgacctggt gccgctcgaa agcgtgtggc tcacccagca gcgtggattc tgggaggccg
    78841 tcgagtcggg cggtgagaac ctgcgaagca agcgcgattt tcagttcatg gtcgatgtgc
    78901 tgcgcgcgcg gcggcagaag gtcacgccgc tgcccgttct ggggattcga aaatgacgcc
    78961 acctttcccg agcgcagacg aacggcagga gatgaatcgc ctcctggtga ataacctgga
    79021 ccaggcggtg gacgatctcg acatcttcac gccggaccgc gagttcgatg ccgaaatctc
    79081 catcagccaa ctccggcgtg ggcggcgcga agctctggct gaccccgtcg ggatgatgac
    79141 actggtggag gtggacggcg tcctcctctg gcaggaggga gcggcggttt cttctcctgc
    79201 gctgcgccgc cgccggggcc ggcgggccga agggccgggt ggcgagatcg tacagcagtt
    79261 caagttcgag aagctcgagc cctccaaggt cggagccttt ctcgaaagga tggacctgaa
    79321 gctgactccg gagcgcgggc ttcgcaaatg ggatggaggc aagctcgcgg cgatcgataa
    79381 gccggccgac aaggggcgca tcctgctgat cgtccacgga acattcagca ataccgacgc
    79441 ggttgtcggc gagctgcggg ccacggccgg cggccaggcg tttctggacc gcgcggtagc
    79501 acattacagc caggtcctga ctttcgacca cccgaccatt tcggtcagcc cggtcttgaa
    79561 cgccctggac ctggcgcgcc tgttcgactc ctccagcgcc gacgtggacg tggtctgtca
    79621 cagccgcggc ggcctggtga cgcgctggtg gatggaggcg ttcgaccgta agcagagggg
    79681 ccgacgggcg gtgttggtgg ggtgcccgct gaacggcacc agcctggctg ctccgccgag
    79741 actgcgtgcg ggcctcagcc tgttgaccaa cgtcggccgc gtactggcgc cggccgcgga
    79801 cgcggcttcc gcggtgattc cgttcctgag cgtcgccgcc ggcctgattc gcgtcctgtc
    79861 gtcgttgtcc agcctggcgg ccgcgtcgcc gatggtggac gccgcggtgg cgatgatccc
    79921 gggcctcgcg gcaatgtcac ggatcgggaa caacccggag ctgtcacggc tgaaccagga
    79981 cccggccagc cccccggccg agtatttcgc cgtccggtcg aacttcgagc cgagcagccc
    80041 cggctgggcg ttctggcgct acttcgtttc gccgaaggag aggctggtgg acgtggctgt
    80101 ggacactctc gtgttcgacg gccagaacga cctggtggtg gacaccgcct cgatgaccga
    80161 gctgcctccg gcgtccgctc cgccggcgac ccgcgtcctg gacttcaaga ccaacgacac
    80221 ggtgtatcac accaactact tccgccagcc ccaaacgcta aaattcatcg cggacagcct
    80281 gcagatcccg tagcagggtg gctgtcccct tttcttccag ccactgagtt cactacagct
    80341 ccaaaccgcg accgccaggc gcaagtctca atttcgggat gtaactgtcc gaatatggga
    80401 ctcgtacgag tcacctcttg cggccccgcg tgccaaaacc gctgaatcgc tgcggcaatg
    80461 accagcaatc acatcgcgag gaaaaggctc aggatcgcga ggcgtgtggc gcgatccggc
    80521 atctcgaggt gcctgggggg aattggtccc gattcggacc atctgctatg gcacgaaacg
    80581 tgctgctgcg tggcaagagc catgatctcg acgatgacgc gggagatccg ctacagcctg
    80641 cgcactctga tcaagagccg ggggttcacc gccgcggccc ttctcgcgct ggctctcggc
    80701 atcggcgcca acagcgctgt tttcagcttg gtcaacgccg tcctgctccg gccgctgccg
    80761 tacgaagatc cggcgggtct cgttgatatc tccgaactcg acgcggagcg gcaagggctt
    80821 ccggtcgact acctcgactt cgtggagtgg cgcgctcagg ccgatgcctt caaacggatg
    80881 gcgtttcagg cgaccttcac gcagacactg acgggcgccg gcctcgccga gaacttgccg
    80941 gtcgggttcg tcacggcaga cttctttggc acgctgggag tgcgcccgat cctcggccgc
    81001 gatttcgtcg cctcggacga tcggaagacc gcgcccccgg tggcgatcct cagctaccgc
    81061 ttctggcaga accgcttcgg cggcgatcgg ggcgtcctcg gacgcacgtt gaggctgggt
    81121 cagaggaatt tcaccatcgt cggggtcctg ccgtcttcct ttcggtacca ccgccctggc
    81181 caggtgtacg tcgctttcgg tccatgcctg agccagtggg gcatggacca gcgcggcaat
    81241 cacaacaacg cgttcgtggt agcgcggcta aaaccaggcg tctcgatcga gcgggcgcgc
    81301 gctcagatgg ataccatcgc ccggcgtctg gaggcacagt tccccttgaa cagggggatt
    81361 ggcgccgccg tccggccgct ccgcgagctg ttcgcgggtg gcgtgcggag ccagatgctg
    81421 atcctgctgg gggcagtcgc cttcgtgctg ctgatcgcct gcgtgaacgt ggccaacctg
    81481 atgctggttc gctcggcggc gcggcggaaa gagattgccg tccgggcggc gcttggcgcc
    81541 aggcggctcc gcctcatcgg ccagctcctc accgaaagca tcctgctttc gatggccgcg
    81601 gccctgttgg ggctgctgct cgccgaggcc gctttcgacg tcctggcgaa gcttctgccc
    81661 tggggcttcg aagcggccga cgtctcgatc gattgggcag tgctggcatt cacgctgtcg
    81721 gtgggttgct ccactggtat cctgttcgga attgtgcccg cgcttcaggc caccgatttc
    81781 tcgctgagcg aagcgctgaa agagagcggc cggttcggcg acgccgcttc ggcgcgaagg
    81841 aaactgcgca gcgccctggt gattgcccag gttgcgatct cgatggtcct gctcgccgga
    81901 gcgggcctgc tcgcaaggag cttttggcgg ctcgtgcagg tcagcccggg tttcgaggta
    81961 caacacgttc ttaccatgga cgtgagctgg ccctattggg agatcttggg cgtggcggga
    82021 gcagtggatt tccacaagcg gctgtgcgag cgcgtcgagg cgctacccgg cgttcgggca
    82081 gtgggcggga tttggccgct gccgctcggc accggcggcg ctgccatccc gatttaccgc
    82141 gccggcaagc cggtgcccgc gcaaggccag tgcccggtgg tggcctacca ccgcgccacg
    82201 ccgggtctgt tttccgccct gggaatcccg ctgttgcgag gtcgcatctt cacagcagcg
    82261 gacggtactc ctcctcggga ccaatccccc gcgcaggtgg aagctgcctg gcgtgcttcg
    82321 actcttgctg ccgtgatcag cgaaagtatg gctcgccgct tctggcccgg tgaagatccg
    82381 gtcggccggc gtttccggtt cgggccgccg gagttcaatg gcccctgggt gcaggtactc
    82441 ggcgtagtcg gcgatatcaa aggcgctggc ctggaccagc ccgccgaagc cgaattctac
    82501 ctctcggcct acctggaccc gaacgacctg acgctggtcg tccggtccgg cggcgacccg
    82561 gcggcgcttg ccgcggctgt ccgtaccgcc atagcggagc tggatcgaaa cgtcccggtg
    82621 gacaacgtgc gtacgatgga caaggttctc gccgatgggg tctcccggcg gcgcacaaac
    82681 atgctgctca tcggcgcgtt tgcgatgttg gccttggtgt tggcagcggt gggtctctat
    82741 ggcgtaatgg cttacctggt cgcgcaacgc cggcatgaga tcggggtccg tattgccctc
    82801 ggcgcttcgg gtgccgatgt cgcttcggcg gtagtgaaag aggccggcct gttggcgctg
    82861 gccggtgtcg gcctcggcac gcttggggcg ctggcgttga cgcgcctgtt ggccaacctc
    82921 ctgttcgggc tggcggcaac cgaccccgcc acattcggcg cggccgccgc tgtgctgctg
    82981 gctgtggctg tcgcggcaag ctgggcgccc gcccgccgcg ccgcccgggt cgatccgatt
    83041 gttgccttgc gctgccaata gccacggcag gaagccgcga ccgcagggag cggtcatttc
    83101 gaagcttccg ttccctacac gcccctggtt tcaaaaccgt gctgcgtgcg gtggtacgaa
    83161 ctgtcgtacg ccttgctcga tttgaacagc tcataacggc cgtcgccgac ggcctcgcca
    83221 acgcggctca ggagcgtcct cgtttcggcg gcggtcaccg gcttgaatgc gcggccgatt
    83281 cggatcgcct cacgtagctg ctccatcgag gtcaacccga ccacctgaac ggcgaccggc
    83341 tggctcagac agtagcggta gcaatcctcg accgacagct tgccttcggt gatgacgcgc
    83401 ccgtcgccgc cgcagccctt catgccgatg atgccgacgt tcttcttcag acagacgggc
    83461 atcacttcgt cccgaaagct gacgaacgcg gaatccatca cgttgttcgg catctgggct
    83521 gtgtcccagt cgaacttctg cgcaagcatc ttcaggtgga tggcgggagc tttgtgaccg
    83581 gtgaagccga tgaaccggac cttgccctgc ttgcgcgcct ccatcagggc gcggatgccg
    83641 cctttctcga agacccagtc cggatcgttg tagtagttgc actcgtggaa ctgcagcagg
    83701 tcgaggtggt cggtctggag ccggcgcagg ctctcttcga gctgctccat ggcgggtttg
    83761 tactcgcggc tgcaaacctt ggtcatcaga aaggcctgct tgcggcgccc gctcacggcg
    83821 agcgcctttc ccatcaggtc ttcgctgcgg ccgttgttat actcccacgc attgtcaaag
    83881 aacgtcatgc cctcgtcgag ggcggtgtgt atgatgcgga tcgcctcggc ctcttccttc
    83941 acacgcccaa cgtgcgcgcc gccaaggccg aggaaggata cacgaacgcc ggtgcggccg
    84001 agcactcgag tgggcaggcc ggtggccgct gcgctggagg tttgagcaat cacttgctcc
    84061 gccagggctg ccgtgccgag ggccgcctgg aagaatacac gacgggttgt tttcggttcc
    84121 atcgcgatta cctcgcgtga agctcaggcg gccttccgca tctcggaggc agccggaatc
    84181 ttcgtcagaa gttccttcag ttcatcccgg ttggccgggc cgatggtgaa gcagtcgaca
    84241 caatcgagcg acatggcgta acggagcgct tcatcgactc gattacgcaa gccgccttcg
    84301 ccgaggatct tcatgccgat aacgccctta ccggcggcct tcatctgacg aaggacggaa
    84361 acaaccgtgt tcgggtcagc gtccatgagt atgctcgccg gattgatgcg ggcgaggtcg
    84421 acgtgaaccc acggcgtctt cgcggcggtg cggagggcct caatcgagtg gcaggaaacg
    84481 ccgtgcgttc gcacgatgcc cttttgtcgg gcctcggaga gcacctccat cgctccagcg
    84541 cgctcctcgg tccaaagagg attcgtgacg gcgtggagca gcatgatgtc gaagtagtcg
    84601 gttccgagct cctggcggaa gcggtcaagg tcggcgcgca tctggtcggc ggtgcgggcg
    84661 cgggtctttg tcaggatagt gaccttctcc cgcttgacgc gtttgagcgc ctctttgacg
    84721 tggggatggg tcttgtagcc gtcggcggta tcccagaaga agatgccttc gtcgtaaccg
    84781 tactggagca tgtccgccac gccctggacg cccagcagtc gctgcacgct gccccccttg
    84841 gtgccggtgc cgatggcgag ccgggagagc ttcaccttgt caggacccag acgcacgacg
    84901 tcggtcgctt tacgtttcgc ccccagcgcc ggagacagcg cactggtggc gagcaagccg
    84961 gtttggagga attgtcttct attcatcatg ttggacactt ctcctttcgg ccggaagccg
    85021 gcgcactccg gccccggact gtgctggaag tatacaccag acgggatcgc gggcgggcga
    85081 tgtggcatgc tctcgtccga atccggaggc tgccgatgaa ccgccgaacc ttcctgacgc
    85141 tgcccctcgc cgcggtagcc gccggtgccg cgccgagccg tccaaacgtc ctcatcctca
    85201 aaccggtggc cagggaggac ctgcatttca ccatcgagtc tcgggatggc tttcacttcg
    85261 cggtgcgccg gggcgaatgg aagctcgtgc gtcatgagcc gcggccgtcc ggcggagcgg
    85321 tcaatcaact gtaccgtctc gacgaggatc ctcaggaaac caaggacgtg gcggcggctc
    85381 acccggcact ggtgaaggag cttgccgcaa gtatcgagaa gtggcgcaag ctgtacccgg
    85441 ccgaggggac ccggctggaa aaccgcccgc cagcgggctg gaaagcaccc agccaatggg
    85501 cggaagcggc gcggaggtga gaatccggac ggaagggcca acggccaagt aaacggtaac
    85561 gcgcccacat ctacgggcta gcgactatcc gtcagcgtga agcgatcaac actgcgccat
    85621 caggcgcggg caatcagcgc agactcgttg caacatcgct gttcattgat ccggatgggg
    85681 cccgatgata ggcttggcgg taacaggaga tctcatggcc cgcacacagg ccgcccctgc
    85741 cctcgatctg ggtcaagcgc tggtggagag ttacgccgta aacgaccgaa tgaaccaggt
    85801 tgtgctcgag tatctcgatc ccggtgcatg gcgcgccaaa ctgcccggca gcaggggacg
    85861 tacgatcgcc gcaatcttct cgcacctgca taacgtccgc cgcaaatggc tgaggctctc
    85921 ggccccgcgt ataaagctcc ctgccccgct cgacggcgct cgctgttccc agaagcaggc
    85981 tagggtagct ctggcggaaa gtgctgcacg ctgttgcgaa atgctcgctg gcgctctggc
    86041 cgggcccgag cgccgcgttg aaacctttcg ccgggacgga tgggcgagac cgtggcccgc
    86101 cggtgcagcg atggtggcgt acatgatttc gcacgaggcc catcatcgcg ggcaggtgtg
    86161 tatgctggca catcagctcg gattccccct gccggtcaag ggcgcctacc ggatctgggt
    86221 ctgggaaaaa ctctggaagg agtgcggatt cacgcacccg cgatgaggca ccccggattc
    86281 ccggtggcgt cctccgtccc ggcgagccac acctgcctgg gcgcgagcga tgccgaacgc
    86341 tgcggaaggt cggtgcgccc gagcgctcct tggacgaggt tggcgagggg gggtctggat
    86401 agagcatcct gcggcctttt gaattccttg gccagaggac ggtggctcgg ccacatctac
    86461 gtgatgacga cttctcgctc aggtggattg cttctgggtt ggcgacggtt cgctcggaat
    86521 ccattgggcc acgttcgcag aatcgggcgt gctcgagccg gcttaacccg tgagaggctg
    86581 gagttccccg agcatcgtgg ggatgagctc ggaaacggta gggtgaatgt gaacggcccg
    86641 ctggatcacc gtatagggcg ccttggcgta catgacgtcc aaaatcgagt gaatcgcctc
    86701 gtcaccgccg acgcctaaaa tggcagcgcc caggatttca cgtgtatcgg catccaccac
    86761 tatcttcatg aagccctggg tttctccttt ctcaatagcc cgtgaaactc tagtcatggg
    86821 gcgattgcca accagcgccc gcctgccggt cttgcgaact tcggcctcgg tcaggccggc
    86881 tcgtccgagc ggcgggtcga tgtacagcgc gtacgccggg atgcggtcag tgatccgccg
    86941 cgagtcgtta tccaggagat tggcggcaac aatctccgaa tcgttgaacg acgtatgggt
    87001 gaaagcacct cttccattac aatcacccat ggcccaaatc ccagggacgc tggtacgcaa
    87061 ttgatcgtcg accacgatgt agccgcgttg gtccaactcg acgccagctt tatccagtcc
    87121 gaggtcgtcg gtatttgggc ggcggcctac agcgagcagc agatgagttc cgctaatctc
    87181 tgagggtccc tcggcgcaat ccaaacgggc aataatctct tcgccccgct tcgaaaaccc
    87241 gatgcatttg gcgttcagcc ggaggcggat gccttcgccc tccagaatct cctggatggc
    87301 cacagataca tcttcgtcct cacgctgtac caggcgcggc cccatctcga cgacagcaat
    87361 gtcgcttcca aatcgacgga acatctgccc gaactccagc ccgacgtagc tgccgcccac
    87421 cacgatcagt cgtgggggaa ggaagtcgat gtccatcatt gagctgttgg tgagatactc
    87481 gacgtgctca agtccgggca tcggcggcac ggccgcgcgc ccaccgacgt tgatgaagat
    87541 ccgatcggca gcgatccttt ccggccccac gctgacgctc cgcggagact cgaaacgcgc
    87601 atggccctgg tagacggtgc aattttcgag ctgcctcagc gactcctcgg ttccgattct
    87661 cgactttgcg gaaatggcat cctttctcgc cttgaccagc cgcatgtcga cgctcacccc
    87721 gccatcgatc acaacgccga aatcccaggt gcggcgcgcc gtgtgagcgg cgtaggcgct
    87781 cgccaccatc gtcttggtag ggatgcagcc ggtgttgacg caggtgccgc cgaacaactt
    87841 gcgttccacg atggcgacct tcatgcctgc cgcggcgagg cggtgcgcca gaaacggccc
    87901 ggcctgtccg gttccgatga tgattgcctc aaattgcttt gtcatagagc gcgggagacg
    87961 gtccagaacg cggcgcaaat cgcaatcgcg tcctcaagga gggcaattgg gagatccttc
    88021 cctcccgttg cctttaccag cctcctgcga aactcgtagc ccgcgagcgt cccagccacg
    88081 cccccaagcg cgccggcaag caggccgcca agcagcgccc cgcttggtgc gccgagcgca
    88141 gctccagaaa acgccccggt aactatccgg gcagtgaacg gcgcgagcgt cttccggctg
    88201 ggcgtcttcg ggagcttgtc attgatcaac tccgcaattg caaacagagt gaggatatac
    88261 ggagtggccg caaacccgag gaaggcgagc ggcgtgttct ccaagtgcag ccagcccagg
    88321 cgggcagccc aactgaccgc ggtcggcggc gtcatggaac ggagtcctgc aacgacgcca
    88381 atgaggaatg ccagcatgta tgtcggcata ggcacctatt agatcacagg cggctgcact
    88441 cttccttacc caaacaggcg tccaactttg tagcaagttg ctgtgtagcg atgtaattgt
    88501 gccgttactt tagccctgaa ttgagttttg gcgaagcaga aacacccgcc gccgttggtt
    88561 ttactggtat cagcaccgga gcccccttgt ccttaactaa aaacacgatg aacttggctg
    88621 gttcggtgct gcttgcgttc cggccaacgg tatgaatgtc gttgggacct tcgtaaaaag
    88681 tctggcctgg cctgagagtg atttctttcc cccctttcaa ctgcatcaca acggttcctt
    88741 ccagcacgta aacgaatgca tgcgcattgt gccgatgaat cggatccgaa gcgccgggag
    88801 gatacacgac agtgatcatc tgaccttcct taccgggaca ttccggaaga tctttcgaca
    88861 tgagtggtgt gacctgagcg tcct
//
LOCUS       DATIFQ010000413         5182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2056805, whole genome shotgun sequence.
ACCESSION   DATIFQ010000413 DATIFQ010000000
VERSION     DATIFQ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056805"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..884)
                     /locus_tag="VLE22_20730"
     CDS             complement(42..884)
                     /locus_tag="VLE22_20730"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB16890.1"
                     /translation="MAIIDSQVHVYEANTPKRPWHSVPNWPDHVTGDEMVAAMDEVGV
                     DGAIFISAFSMYRYDASYAVEVQRAHPGRFALVKPVDPDNPAVADVIADWKMTPGTVG
                     IRIFMTKEVGRNPNDPGLDRILREAVRHDFPVNILCWGNLDAGTALIDRHPDTRFIID
                     HLGIMQPRTPPAPPQPWADLPKVLELAKRPNAVIKISGACTLSREPYPFPDIWGPLAR
                     VFDAWGFDRCLWGTDWTRAFAVVNYEQAVEPFRQTQRLSDSERAMLMGGACAKAYGWS
                     PRKE"
     gene            complement(1239..1487)
                     /locus_tag="VLE22_20735"
     CDS             complement(1239..1487)
                     /locus_tag="VLE22_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16891.1"
                     /translation="MDDDLRSEYDLSQLRGGVRGKYYRQAMAGTNLVLIDPELANVFP
                     NTESVNRALRLLVETAEAAAAPLPRRRRAPNKRLNRTA"
     gene            complement(1480..1770)
                     /locus_tag="VLE22_20740"
     CDS             complement(1480..1770)
                     /locus_tag="VLE22_20740"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HSB16892.1"
                     /translation="MIYEWDPTKAKVNLRDHGVSFEEAVTVFLDPLAVTYPDPDHSDE
                     EDREITIGHSVKQRALFISHCQRGDRIRIISARKATRKEREQHEEGIGEENG"
     gene            complement(1996..2481)
                     /locus_tag="VLE22_20745"
     CDS             complement(1996..2481)
                     /locus_tag="VLE22_20745"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB16893.1"
                     /translation="MVRPAKNTICLWYDGGAEEAARFYAKTFPDSSVGAVHLAPGDFP
                     SGKKGDVLTVEFTVMGIPCLGLNGGPAFKHNEAFSFQVATTDQAETDRYWNAIVGNGG
                     QESACGWCKDKWGLSWQITPIALTKAVTDPDRAAAKRAFDAMMTMRKIDIAAIEAARR
                     G"
     gene            complement(2768..3106)
                     /locus_tag="VLE22_20750"
     CDS             complement(2768..3106)
                     /locus_tag="VLE22_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16894.1"
                     /translation="MMRRFLWRLTAAPDLLLLALLVAIMWHEWYTIGVVGDPDVVKGY
                     YFGSEAMIAHGGRHYASAETYAHSALISGLLFTPVLLAFIAAEARGNKMFTLVSYGIL
                     CLAVGIHWLL"
     gene            complement(3196..3441)
                     /locus_tag="VLE22_20755"
     CDS             complement(3196..3441)
                     /locus_tag="VLE22_20755"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HSB16895.1"
                     /translation="MIPRVEVAHYVQGFTIHLRFSDGSEGDVDLGGELYGEIFEPLKD
                     QALFRQFVVHPEFHTLSWPNGADLAPEFLYEKVRVPA"
     gene            complement(3446..3703)
                     /locus_tag="VLE22_20760"
     CDS             complement(3446..3703)
                     /locus_tag="VLE22_20760"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HSB16896.1"
                     /translation="MPEISRFLGIVIGIFPRDHPPPHFHAVYGDYQITVGIQSGVVSG
                     DFPKRALRLVIEWLDLHKEELLEDWHLVQAGRPANKIAPLE"
     gene            complement(3822..4259)
                     /locus_tag="VLE22_20765"
     CDS             complement(3822..4259)
                     /locus_tag="VLE22_20765"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB16897.1"
                     /translation="MGMIGKGQFRFSYFTPEYDATVAFYRDDLQLVVLESWDRSTDDR
                     GTLFAAASGIIEVLLRSDAQPSSHRWDDRPPQGAFMVIEVPDVEQAYRRAAERRLPIQ
                     QQLTTQPWGHRSFCLREPNGLILYLFSEVEEAAQQRVEPGAGR"
     gene            complement(4361..4654)
                     /locus_tag="VLE22_20770"
     CDS             complement(4361..4654)
                     /locus_tag="VLE22_20770"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HSB16898.1"
                     /translation="MKKTDTRITQGSGNVFLDLGFPPHEAAVMLLRCELAEALRQWIA
                     RQGFTQAQAAKRLGVVQPRISEIARNKVDKLSLDYLVGLCAKAGVSVAVRLAA"
     gene            complement(4641..4973)
                     /locus_tag="VLE22_20775"
     CDS             complement(4641..4973)
                     /locus_tag="VLE22_20775"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSB16899.1"
                     /translation="MQALRFVGTAREDLAAFPEAARRRAGYDLFMVQVGRGPADFKPV
                     RRVGPGAYAIRVRDKAGAFRVIYVAKFERTVYVLHAFQKRTRKSAPADIELAARRYRL
                     IEGGHEED"
BASE COUNT          984 a         1641 c         1536 g         1021 t
ORIGIN      
        1 cgggaggctt tgatgacgtc ttcagtgcgc ccgaggatga attattcttt cctcggcgac
       61 cacccatacg ctttagcgca cgcaccgccc atcagcatgg cccgttcgct gtcgctcagg
      121 cgctgcgtct ggcggaaggg ctcgacggcc tgctcgtagt tgacgaccgc gaaggcgcgg
      181 gtccagtcgg tgccccacag gcagcggtcg aaaccccagg catcgaacac gcgggcgagc
      241 gggccccaga tgtccgggaa aggatacggt tcccgggaca gcgtgcaggc gccgctgatc
      301 ttgattaccg cgttcgggcg cttggccagc tccagcacct tcggcaggtc ggcccaaggc
      361 tgcggcggag cgggtggcgt gcgcggctgc atgatgccca gatggtcgat gatgaatcgc
      421 gtgtcggggt ggcggtcgat cagcgccgtg cccgcgtcca ggttgcccca gcacagtatg
      481 ttgaccggga agtcgtgccg aacggcttcg cgcagaatcc ggtcaaggcc cgggtcgttc
      541 gggttgcgcc ccacctcctt cgtcataaag atgcggatgc cgaccgtgcc cggcgtcatt
      601 ttccagtcgg cgatgacatc ggccaccgcc ggattatccg gatcgaccgg cttgacgagg
      661 gcgaaccggc cgggatgggc ccgttgcact tccaccgcat agctggcgtc gtaccggtac
      721 atggaaaagg cggagatgaa gattgcgccg tcgacgccga cctcgtccat cgccgccacc
      781 atctcgtcac ccgtgacgtg atcgggccaa ttcggcacac tgtgccaggg ccgtttcggc
      841 gtgttcgcct catagacgtg gacctgggaa tcgatgatcg ccatcgggga agctcctctg
      901 tgccaagcga tttcctggta ttagaacagg tcgcgcccac cgcgtccaga agggggccgg
      961 gcgctgtgcg gtctgatgcc ggcggaccgt ccctcctcgc cctgaccctc tccttccgga
     1021 aggagcgtgg gtctaccacc gctgtgttac aggacttccc agtcaagtcc atctcgcgca
     1081 tcatgagtat gctgattccc ttgcgaaccg acaaggtgag tggccgctta ctgaaagtgt
     1141 gtataacggt ggtgggtcgc aagcagccgt cattggcgct gaccgttttc gttgctggca
     1201 tagtcccacc gaacgttgcg gctaagcggc cgcgtccttc aagcggtccg gttgagccgc
     1261 ttgttcggcg ctctccgccg acggggtagg ggggcggcag ccgcctctgc ggtttcgacc
     1321 aggagccgga gtgctcgatt cacggattcc gtgtttggga agacgttcgc gagttcgggg
     1381 tcgatcaaca cgaggttggt gcccgccatg gcctgtcggt agtacttacc gcgaacgccg
     1441 cctctgagct gagaaagatc gtactccgac cggaggtcgt catccattct cttcgccgat
     1501 gccttcttca tgctgctcgc gctcctttcg ggtcgccttg cgtgcactga tgatccgaat
     1561 acgatctcct cgctgacagt gggagatgaa cagggctcgc tgtttcacgg agtggccaat
     1621 cgtaatctct ctgtcctctt catcggagtg gtccggatcg ggatacgtca cagccaacgg
     1681 gtcaagaaac accgtcaccg cctcttcgaa agacacccca tggtcacgca gattgacttt
     1741 cgccttcgtc ggatcccatt catagatcac ggactgagtc tacctcaact tggccgacaa
     1801 gccgaacgct atgcggctca cctgcggcgg ctgcttactc aactcgggcc accccaaatt
     1861 ctggcgaggc cgccgccagg tgcagccgcg ggttatgtcg gggccgtgcc aaagcgcgcc
     1921 atgatcaata accgtagatt tccgccttga cgttcccctt gcgcctccga gatgcacgct
     1981 tcgaagactg caacctcaac cgcggcgggc cgcctcgatt gcagcgatgt cgatctttct
     2041 catcgtcatc atcgcatcga acgcgcgctt ggcggcagcg cgatcgggat cggtgaccgc
     2101 tttggtcaga gcgatcggcg taatctgcca ggacaagccc catttgtcct tgcaccagcc
     2161 gcacgcactc tcttggccgc cgttgccgac gatcgcgttc cagtaacgat cggtttcggc
     2221 ctggtcagtg gttgcgacct gaaacgagaa tgcttcgttg tgcttgaacg cgggtccgcc
     2281 attgagcccg aggcagggaa ttcccatcac ggtaaactcg accgtcaaca catccccttt
     2341 cttcccggac ggaaagtctc ccggcgcgag gtgcaccgcg cctacggatg aatcgggaaa
     2401 ggtcttggcg taaaatcgcg ccgcctcctc ggcgccgcca tcgtaccaaa ggcaaatcgt
     2461 attctttgct ggcctcacca tgtcactcct ccatcttgat gagccgcccg acgccgcagt
     2521 aagacgatgc gctcgcggtc gaagggcatt ggcttggtcg tcttccgcgt taatgggctg
     2581 agaaatccgt actatatgag ccgatacgca tgcctagacc aatcaaacca ccataccgtc
     2641 ggaatgtcga gcgactcctg cgacataact acgctatgcg cttaacctgc gcgccgccag
     2701 attccgcggg caaacgcgag cctctggtgc gtcacgttga agcgcgtgtt gtgcggccct
     2761 catgcgtcta tagaagccag tgtattccaa cggcgagaca gagaatacca taggacacca
     2821 acgtgaacat cttgttgccc ctggcctcag cggcgatgaa agccagtaat actggcgtga
     2881 acagcaaacc tgagatgaga gcggagtgtg cgtacgtttc tgcactggcg tagtgccttc
     2941 caccgtgggc aatcatggct tccgagccga agtagtaacc cttgacgacg tctgggtcgc
     3001 cgactacacc tattgtgtac cactcgtgcc acatgatagc aacgagaagg gccaggagga
     3061 gcaggtctgg tgcagcagta agtctccaaa gaaaacgacg catcattgta gtcaaggtgg
     3121 cctccgagac cgcctaacgt ccggcatcac cggcgctgcg cggatgtctg cgcagccccc
     3181 gagtgcatgc cgttgttatg ctggaactcg caccttctcg tacagaaact ccggggcgag
     3241 atccgctccg tttggccagg agagtgtgtg aaactcgggg tgtaccacga actgccggaa
     3301 cagagcctga tctttgaggg gctcaaagat ttccccgtat agctccccac caagatccac
     3361 atcgccttcc gatccgtccg agaaccgcag atgaatcgtg aaaccttgca cgtagtgtgc
     3421 tacctcaact ctcggtatca tctcgctact ccaacggcgc aatcttgttt gccggtcgtc
     3481 cggcttgaac aaggtgccaa tcctcaagga gttcctcttt gtgaaggtcg agccactcga
     3541 ttacgagccg caatgcacgc ttcgggaaat caccgctcac tacgccgctc tggataccga
     3601 ccgtgatctg atagtcgccg tacacggcgt ggaaatgcgg cggtggatgg tcacgcggga
     3661 agatgccgat cacgatgcca aggaatctgg atatctcggg catacaagaa tcttacctct
     3721 cgtccgcctt ctcatccaca gcataaacac cgcgctaacc tgcgaagggc gcatttcgac
     3781 gacctcgcgc cgccccggcc aagccaagcg gcgcgcgcac cttagcgccc tgcgccaggt
     3841 tcaacgcgtt gttgggctgc ctcctcaacc tcgctgaaca ggtagagtat aagcccgttc
     3901 ggctcccgaa gacagaagct gcgatgcccc cagggctggg tagtcagctg ctgttgaatc
     3961 ggcagacgtc tctccgccgc ccttcgatac gcctgctcga cgtccggaac ctcgatgacc
     4021 atgaacgcgc cctggggggg tcggtcatcc caacgatggg acgagggttg ggcatcggat
     4081 cgcaacagca cttcgatgat tccagaggct gctgcgaaga gagtgccacg atcgtcggtg
     4141 ctgcgatccc aagattcaag caccacaagt tgcagatcgt cgcggtagaa ggccacggtc
     4201 gcgtcgtact ctggggtgaa gtagctgaag cgaaactggc ccttacctat catgcccatc
     4261 aagccattca ggcgcctgtt cttaatgttg ggccaacgtg acggcggcta agccgtggcc
     4321 cgcagggcca gcggttccag ccgcgggttc ggcccaccga ctacgctgcc aatctcaccg
     4381 cgactgagac gccggccttg gcgcacaggc ccaccaggta gtcgagcgag agcttgtcaa
     4441 ccttgttgcg tgcgatttcg gaaatgcgcg gttgtaccac cccgaggcgc ttagccgcct
     4501 gcgcttgggt gaagccctga cgggcgatcc actgccgcag ggcctcggcc agctcgcagc
     4561 gcagtagcat cacggccgct tcgtgcgggg ggaagcccag atcgagaaag acgttaccgc
     4621 tcccctgggt aatgcgcgtg tcagtcttct tcatggcccc cctcgatcag cctgtaccgc
     4681 ctcgcggcca gttcgatgtc cgccggcgcg cttttccgtg ttctcttctg aaaggcgtgc
     4741 agcacataga ccgttcgctc gaacttcgcc acgtagataa cccggaacgc gcccgctttg
     4801 tctcggacgc ggatcgcgta ggcacccggc cccacgcgcc gcacgggctt gaaatccgcc
     4861 ggtcctcgcc ccacttgcac catgaacagg tcatacccgg cgcgtcggcg tgctgcctcc
     4921 gggaaggctg ccaagtcctc ccgggccgta ccgacaaagc gcagcgcctg catgatgcag
     4981 gtagtatacg aatattcgta tagctggtca agcgcgttca ggccgaacgc gcgcggcgat
     5041 gcgcgggccg agaggcgcgg cgcttgcccc gcaaacgccg tgacgcgagg acaggcgcgt
     5101 cgaccgcata gctaggttgc agcacgaatg cgcccgctct tgagcaacca cgccaagtac
     5161 ccggcggccg taacatcgta cg
//
LOCUS       DATIFQ010000414         4227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_715717, whole genome shotgun sequence.
ACCESSION   DATIFQ010000414 DATIFQ010000000
VERSION     DATIFQ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715717"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VLE22_20780"
     CDS             <1..440
                     /locus_tag="VLE22_20780"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB16900.1"
                     /translation="AGGHNVLMIGPPGSGKTMLAKRLAGILPPLTFEEALETTKIHSV
                     AGILQKGTGLLRSRPFRAPHHTVSDAGLIGGGAGTPRPGEVSLANHGLLFLDELPEFA
                     RNVLELLRQPLEDGSVTIARANMTLSFPANFMLVAAMNPCPCR"
     gene            complement(736..1485)
                     /locus_tag="VLE22_20785"
     CDS             complement(736..1485)
                     /locus_tag="VLE22_20785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB16901.1"
                     /translation="MGTNIEGKVVVITGASSGLGESTARHLAKLGAKVVLGARRKDRL
                     DAIVKDIQAAGGEALAVTVDVTKRAEVEGLVKKAVDTYGRIDVMMNNAGIMAIAPLAA
                     LKVEEWERMVDINIKGVLYGVAAALPHMQKQESGHFINTASVFGIKVFAPGGTVYCAT
                     KEAIRTLTEGLRIEAHADKIRCTIISPGAVDSELKQDTSDPASLEAVKGLYKAWAIPA
                     ESVARAVAYAIEQPPDVDINEIVIRPTAQDF"
     gene            complement(1537..2118)
                     /locus_tag="VLE22_20790"
     CDS             complement(1537..2118)
                     /locus_tag="VLE22_20790"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSB16902.1"
                     /translation="MRSDIVPGGVFPDYELPDHTGTLRKLSGLQGDDPLILTLARGHY
                     CPKEHQQHQELAAFYPKVAVAYTQIATISTDDHHTLQEFRASVGAQWTFLSDPDRKIQ
                     KDLDIQEYTDPEHNPMIPHTLVLKPGLVIHRIYNGYWFWGRPSVVDLWHDLRAVTSEI
                     RPDWDLYTPGLREAWNAGDFSPFHGWNKRTKSD"
     gene            complement(2141..3904)
                     /locus_tag="VLE22_20795"
     CDS             complement(2141..3904)
                     /locus_tag="VLE22_20795"
                     /EC_number="2.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC and FAD-binding oxidoreductase
                     domain-containing protein"
                     /protein_id="HSB16903.1"
                     /translation="MPKLLAVNIGLPQNIQWRGETVHTGIWKHPVQGRCAARRLNLEG
                     DGQGDLAGHGGEQRAVMVYQIDSYRYWERTLGRRDFTFGQFGENFTVEGLPDDEVCIG
                     DRYRLGSALFEVTQPRVTCYRVGIRMDEPRMAALLVAHHRPGFYLRVLEEGEVGAGDE
                     FVKVAEGPERFTVADIDALLYLPGHRPDQLQRALRIPALSPGWKGSFEALAAQDGGNA
                     PASGNRGLSPAAGKPPAWRGFRPLRVARVERESAGVTSLTLESEDGSALPPALPGQFL
                     VLRLQPAANLPVILRNYSMSGAPGAGTYRVSVKQEANGKGSTFLSTQVKRGDVLEVSA
                     PRGTFTLQPGEEPVVLLSAGIGATPVLAMLHSLAANRSAREVWWLYGARNAAEHPFAR
                     EVLDLAASLPHFRRFVAYSRPAPEDRLGEDYDAPGRLSLTVLQQLGVPSQADFYLCGP
                     PAFLRSFAQDLTNWGVASGRLHQEVFGPEQSVTPGISKAPARPPHPPAGVPGPGPMVS
                     FARSGLDVPWSPAYHSLLEFAETCDVPVKWSCRTGVCHTCECALIGGSVRYDPEPLDP
                     PSDGNVLICCSRPESPVELDL"
     gene            4038..>4227
                     /locus_tag="VLE22_20800"
     CDS             4038..>4227
                     /locus_tag="VLE22_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16904.1"
                     /translation="MRKEFETHLTWAPSLDAAVPTGTISRTVNNLIRFELDAIHAAKV
                     RNVSKVGSHANLLSRGNGR"
BASE COUNT          803 a         1343 c         1275 g          806 t
ORIGIN      
        1 cggcgggcgg ccacaacgtg ctcatgatcg gcccgccggg ttcgggcaaa acgatgctcg
       61 ccaagcggct cgccggaatc ctgccgccgc tgaccttcga ggaagcgctc gagacgacca
      121 agatccatag cgtggcaggc atccttcaaa agggcacggg cctgttgcgc tcccggccgt
      181 tccgcgcgcc acaccatacg gtttcggatg cagggttgat cggcggcggc gcgggtactc
      241 cccgccccgg cgaagtgagt ctggccaacc atggtctgct tttccttgac gagctgcctg
      301 agttcgcgcg aaacgttctc gaactgctcc gtcagccgct cgaagacggc tcggtgacca
      361 tcgcccgcgc caacatgacg ctctccttcc ccgccaactt catgctcgtg gcggcgatga
      421 atccgtgccc gtgcagatag caactcaaca cgggttgaac cgcgagcagc ggattatgaa
      481 ccgagtacag cagccagcgg accggtccgc tggctgcaac acggccgaag tggaaccagc
      541 atcagtccta ttctgctgtc aaagcgagtt cgaatctaag gtggggagcc aattctcaac
      601 cctctgattc cattgacaac actgtgggcc aaagccgcta agtcccttgc catcaggttc
      661 gaatcctctc tggggtaggg gaaccggcag accacttctc tactttctac caaccccggc
      721 cgaagggttg atggctcaga agtcctgcgc cgtcggacga attacgatct cattaatatc
      781 gacatcgggt ggctgctcga tggcgtaggc cacagcgcga gcaacggact cggcgggaat
      841 ggcccaagcc ttgtacagcc ctttgaccgc ctccaaagag gccggatcgg aggtgtcttg
      901 cttcagttcg gagtcgaccg ccccagggga gatgatcgtg caacgaatct tgtcggcatg
      961 cgcctcgatg cgcaggcctt cggtaagggt tcgtatcgcc tctttggtcg cgcagtaaac
     1021 agtgccgccg ggtgcgaata ccttaatgcc gaaaaccgaa gcggtgttga tgaagtgtcc
     1081 gctctcctgt ttctgcatgt ggggtagagc ggcagcgaca ccgtagagca cgcccttgat
     1141 attgatatcg accatgcgtt cccactcctc taccttgagg gcagcaagcg gcgcgatcgc
     1201 cataatgccg gcattgttca tcatgacgtc gatccgcccg taagtgtcta cggccttctt
     1261 cactaagcct tcgacctccg cccgcttggt cacatcaacg gtcacggcca gtgcctctcc
     1321 gccagcggcc tgaatatcct tcacaatcgc gtccagacga tccttacgcc gcgctccaag
     1381 aacgaccttt gcgcccagtt ttgcaagatg gcgagcagta ctctcgccga gaccgctact
     1441 tgctcccgta atcacaacga cttttccttc aatgttcgtg cccaagttag tttcctttcg
     1501 tgttgatcat ttgatatcct caccggacca agatcgttaa tccgatttcg tacgcttatt
     1561 ccatccatgg aagggcgaga agtcgcccgc gttccatgcc tcgcggagcc cgggggtgta
     1621 caaatcccag tcggggcgga tctcgctcgt cacggcacgc aggtcgtgcc agaggtcgac
     1681 gacggacggg cgcccccaga accaatagcc gttgtagatg cggtggatga ccaagcccgg
     1741 cttgagcacc agcgtgtgcg ggatcatcgg gttgtgctcg gggtcggtat actcctggat
     1801 gtcgaggtcc ttctgaatct ttcgatccgg gtcggagagg aaagtccatt gggcgccgac
     1861 cgacgcacgg aactcctgga gcgtgtggtg gtcgtcggtg gagatcgtgg cgatctgagt
     1921 gtaggccacg gcgaccttcg ggtagaaggc ggccagttcc tgatgctgct gatgttcctt
     1981 ggggcagtag tgtccgcgcg cgagtgtaag gatcagcggg tcatcgccct gcaatccgct
     2041 cagcttgcgt agtgtcccgg tgtgatcggg cagctcatag tcgggaaaga cgcctccggg
     2101 cacgatgtca gaacgcattg ttggcctcct tcgattggga tcaaagatct aactccacag
     2161 gcgattccgg ccgagagcag cagatcagca cattcccgtc ggaaggggga tcgagcggtt
     2221 ccgggtcgta ccgcacggac ccgccgatca atgcgcactc gcacgtatgg cagactcccg
     2281 tccggcacga ccacttcacg ggaacgtcgc aggtctcggc aaattcgagc aggctatggt
     2341 atgcaggact ccacggcaca tcgagtccgc tgcgcgcgaa ggagaccatc gggccggggc
     2401 ccgggactcc cgccggcgga tgcggcggcc gggcaggagc cttggagatg ccgggcgtca
     2461 cggattgctc gggcccgaat acctcctggt gtaatcgccc gcttgcgacg ccccagttcg
     2521 taagatcctg cgcaaagctg cgaaggaacg ccggcgggcc acacagatag aagtccgctt
     2581 ggctcggcac tcccagttgt tgaagcaccg tgaggctcag acgtccaggc gcgtcgtagt
     2641 cttcgcccag ccgatcttca ggcgccggtc tgctgtaagc gacgaatctt cggaagtgcg
     2701 gaagcgaggc ggcgaggtcg agcacctcgc gggcaaaggg atgctcggcc gcatttcgcg
     2761 cgccgtacag ccaccaaacc tcgcgcgcgg accggttcgc cgccagagaa tggagcatcg
     2821 ccaacactgg ggtcgcgccg atccctgcgc tcagcagcac cacaggttct tcaccgggct
     2881 gcaaggtgaa ggttcctctc ggcgcgctga cttcgaggac atcgccgcgc ttgacctgcg
     2941 tagacagaaa ggtgctgccc tttccgttcg cttcctgttt gacgctcacc cggtaggtgc
     3001 ctgcgcctgg cgcgcccgac atcgaatagt tgcgcaggat gaccggcagg ttcgccgcgg
     3061 gctgcaaccg cagcacgagg aattggcccg gcagcgcggg gggcaacgct gatccgtcct
     3121 ccgattcgag cgtcaacgaa gtcaccccgg cgctttcccg ctcgacccga gcgacgcgca
     3181 acggccggaa ccctcgccat gcaggaggct taccggcggc agggctcaag ccgcgatttc
     3241 cggacgctgg agcattgccg ccgtcctgcg ccgctaacgc ttcgaacgaa cccttccatc
     3301 cgggactgag cgccggtata cgcagcgcgc gctggagttg atcggggcga tgtcccggaa
     3361 gataaagcag cgcgtcgatg tccgcaaccg tgaaccgctc gggcccctca gcgactttta
     3421 cgaactcgtc gccggcgcca acctcgccct cttccagcac acggagatag aatcccggcc
     3481 ggtggtgcgc taccagcagg gccgccatcc gcggctcatc catgcgaatc ccgacgcggt
     3541 agcacgttac gcgcggctgc gtcacctcga acagcgcact gccaaggcga taccggtcgc
     3601 caatgcagac ctcgtcgtcc ggcaggccct ccaccgtgaa attctctccg aactgcccga
     3661 aggtgaaatc gcgacgacca agcgtgcgct cccagtatcg atacgagtcg atctggtaga
     3721 ccatcacggc gcgttgctcg ccgccgtgac cggcaagatc tccctgtccg tctccctcga
     3781 ggttgagccg ccgggccgcg cagcgcccct gaacaggatg cttccagatg ccggtgtgca
     3841 cagtctcacc gcgccattgg atgttctggg gcagaccgat gttgaccgca agcaacttcg
     3901 gcatgaggaa cgcgtcactc gatcaatccg gcttatcgat cgcgaacgga gtgctcacgc
     3961 aatatcatag ctcgctttct tgtgcctgat ccgccgctat cgggcgattc cgagagcgtc
     4021 ggccatctgg gacttgagtg cggaaagagt tcgaaacaca tctgacctgg gcgcccagtc
     4081 tggatgcagc ggtccccacg ggcactatct cgcgtaccgt caacaatttg atccggttcg
     4141 agctggatgc tattcacgcg gcaaaagttc gaaacgtgtc caaggtgggg agccacgcta
     4201 atctcttgtc gcgcggcaac ggccgtg
//
LOCUS       DATIFQ010000415        40744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1290240, whole genome shotgun sequence.
ACCESSION   DATIFQ010000415 DATIFQ010000000
VERSION     DATIFQ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 40744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40744
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290240"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..377)
                     /gene="thiS"
                     /locus_tag="VLE22_20805"
     CDS             complement(153..377)
                     /gene="thiS"
                     /locus_tag="VLE22_20805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HSB16905.1"
                     /translation="MADPAPETIRVVVNGQSRSVATGQTVLGLLRSLGLDPSRLAIEL
                     NGRIVRQTEWAATPLEAGAQLEIVHFVGGG"
     gene            477..884
                     /locus_tag="VLE22_20810"
     CDS             477..884
                     /locus_tag="VLE22_20810"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSB16906.1"
                     /translation="MPTSYPELYFPIIVQAVIAMAVAAGMVGASYLFGKRVRNKVKDM
                     PYECGIVPTGTARERVSVKFYLVAMIFILFDVEAIFLYPWAVVFRELRLFAFVEMFIF
                     VALILAGFFYIWKMGALDWGPEKRSGDSGRHAS"
     gene            874..1362
                     /locus_tag="VLE22_20815"
     CDS             874..1362
                     /locus_tag="VLE22_20815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSB16907.1"
                     /translation="MLPENLRENSVASAVAASAPDAIASGKIDHGELTLEIRPEKIAA
                     VCAILKHQLSFVRLSDVTAVDWYPREPRFEVVYHLHSLETNSRLRLKCPLNSEAAEID
                     SVTGVWRSANWYEREVFDLFGIRFRNHPDLKRIMMPDDWEGHPLRKDYPVHGYRYEYQ
                     NE"
     gene            1372..2592
                     /gene="nuoD"
                     /locus_tag="VLE22_20820"
     CDS             1372..2592
                     /gene="nuoD"
                     /locus_tag="VLE22_20820"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HSB16908.1"
                     /translation="MSDPATSIDNTTPAETGTRPRSMVLNMGPQHPSTHGVLRVLLEL
                     DGETILKAECDIGYLHTGIEKQCEALTYQQAVTLTDRVDYLANLSNNLCYCLAVEKLL
                     GVEAPPLAQWMRVMLTELTRICSHLVWLGTHALDIGAMSMMLYCFRDREDLLRIFEMF
                     SGQRMMTSYFRVGGLALEPPRGWQHPVKKFLDAFPSKIDEYEDLLTANPIWLQRTKGV
                     AVVPLDELLDLGVTGPMLRAAGLNWDIRKTEPYSGYEKFDFEVPVRTESDVYARYQVR
                     IEEMRQSVRICWQAMEGMPSGSWVADAPNVVLPEREKMKTQMEALIYHFKIVTEGFRV
                     PAGEVYQTIESPRGELGYYVVSDGTSKPYRVHIHGPSFGNLQATPRMIEGGLIADVIA
                     ALGSLDFILGDVDR"
     gene            2589..3074
                     /locus_tag="VLE22_20825"
     CDS             2589..3074
                     /locus_tag="VLE22_20825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HSB16909.1"
                     /translation="MTFSPDMEAKFEKLVRSYPPGRQRSALIPMLLYAQDEIGSVTPE
                     LVDEVARRLGITPVEVDGVVTYYSMLRKKPMGKYHLQICTNISCMLTGGTELWEHACR
                     KLGIGNKEVTPDGQFSLEEVECIGACSWAPAIQVNYDFQHHLTPEKLDRLIESLRKAP
                     Q"
     gene            3077..4387
                     /gene="nuoF"
                     /locus_tag="VLE22_20830"
     CDS             3077..4387
                     /gene="nuoF"
                     /locus_tag="VLE22_20830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HSB16910.1"
                     /translation="MLYNEPSPLEVKVLTRRFGLPDSASLDTYLASDGYAALRKALGM
                     KPEEILEQMKRSVLRGRGGAGFPAAMKWGFVPRSSPKPKYIVANADESEPGTCKDRLL
                     MENDPHQLIEGMMIAGLVVDAHAGYIYIRGEYRYLIEILDRAIAEAYERGYLGKNILG
                     SGFDFDLYTHTGAGAYECGEESALLESLEGKRGIPRIRPPFPAVVGAFQCPTVLNNVE
                     TLSAVPAIIRDGGVAFSALGTPKNGGTRLFCISGHVNKPGVYELPMGFNLLRMIDEVA
                     GGVRDGRKLKAVIPGGSSCPVLKAEECDIAMDFDSLSKAKSMLGSGGVVVMDEATCMV
                     KAALRIMRFYAHESCGWCIPCREGTLWLRKILTRFHEGQGRPSDIPLIGELAKNMLGR
                     TFCPLGDAAAMPTISFVEKFRDEFEAHLDGKPCPFEEADRTVLA"
     gene            4387..6480
                     /gene="nuoG"
                     /locus_tag="VLE22_20835"
     CDS             4387..6480
                     /gene="nuoG"
                     /locus_tag="VLE22_20835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HSB16911.1"
                     /translation="MADLVRLEIDGRMVEVPPGTLVINAAKQAGIEIPAFCYYEGLAL
                     QAACRMCLVEVERMPKLIASCTLPVSEGMVVRTETPQVVEARKSMLEFLLTNHPLDCP
                     VCDKGGECELQDMAFRYGTGESRFTEVKHHVEEQQWSPVVFFDAPRCILCYRCVRICS
                     EGMGVSALGVINRGASAEIAPNCDDHLECDECGMCIDICPVGALTSGMYRYKTRPWEM
                     KHEGTVCAHCSNGCKTTLGVYDNRIMRGNNRDRSGINGEFLCIKGRYAFDFVSHPERL
                     QSPLMKIDGRLQEVPWSQALSAVAGRFSEIKARGGKIGVIGSTRTTNEENYYLQKFAR
                     VGLGTNHIDHHRSGDVATLLTALGGRSDALATTDDLYGRKAFLIVAADLAQQHPFLAF
                     QIRANWRHHNCRTYTVTPGPVREDSYAVRSVQVPEGEELDAVEALRDSLAAEPELVVL
                     FGDTIKCDAVRRLVAFGDSLGIPVKYVCLVDYSNSRGAMDMGLLPDLLPGYRPVTEAG
                     LEPGMTLPEILAAPDLAGLWVVGANPLTNKQLVSDAAFVVVQDLFLTETAQRADVVFP
                     AASAYEKSGTVTNVCGEVQRLKRAVSVTGAKPDLEIFGLLAKEMKLDLGIWTPDAVFK
                     EIRDAVRGYNVPLPVIVTGGASQTAPEGGPAGATSRPDLIQSARDTLFTSGSLSRFSK
                     TLNSVTERPGALYRG"
     gene            6521..7714
                     /gene="nuoH"
                     /locus_tag="VLE22_20840"
     CDS             6521..7714
                     /gene="nuoH"
                     /locus_tag="VLE22_20840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSB16912.1"
                     /translation="MSFLSLSLLKATVVAAVLLTTLAYLQWVERKVIAHIQLRLGPSR
                     VGPHGLLQPVADVIKLATKEGFIPPHANKFLYLFAPFLAVTLALLSISVIPFGPEIEI
                     FGIRTEMQLTNLNIGVLFLLAVSSMSVYSVALAGWASNNKYALLGGLRSAAQMISYEL
                     PLAVAVVSVLLLANTLSLRGLVESQSGFYIGFIPRWNVFQLPFPQVFAFLIFLVAAFA
                     ETNRIPFDMPEAENELVAGFHTEYSSLKFAAFFMAEYSNMITGTCMATLLFLGGWNPP
                     VPTGLGANYFPSLVFAAGGAVALYHGLHPARSRDRYSLPVVALVFFLVAAMLLIPVVQ
                     GILAPVFWFGAKVGFLLFAFIWVRGTLPRFRYDQLMRFAWKFLFPVAVLNLLVTGLLV
                     AVVSK"
     gene            7717..8217
                     /locus_tag="VLE22_20845"
     CDS             7717..8217
                     /locus_tag="VLE22_20845"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSB16913.1"
                     /translation="MDVILFLVFAGIAVVCAVNLVVQTHPIASAISLVGVMGSLAVLY
                     LLLGGEFIAAVQVIVYAGAIMVLFVFVIMLLNAGAEQKSGGSLTARYLGIPLLIVLLG
                     LLSFLIRIMLPPSEGVRFGGFTGGSPEAVGRVLFTRYLLPFEVTSVLILVAILGAIVL
                     ARKELD"
     gene            8220..8528
                     /gene="nuoK"
                     /locus_tag="VLE22_20850"
     CDS             8220..8528
                     /gene="nuoK"
                     /locus_tag="VLE22_20850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004321.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSB16914.1"
                     /translation="MTAVPLSWYLTLSAILFALGVAGFLIRRNIITVFMSIELMLNAV
                     NLSFVAFSYQLKQVDGHIFTFFVMVVAAAEAAVGLAIILTVFKNRGTLQIDEVSSMKY
                     "
     gene            8533..10383
                     /gene="nuoL"
                     /locus_tag="VLE22_20855"
     CDS             8533..10383
                     /gene="nuoL"
                     /locus_tag="VLE22_20855"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSB16915.1"
                     /translation="MLHLWLIPALPLAGFLVNGLFGRRMPKALVDAVAIGSVLLSFLW
                     VLRTLSALGTMEAAYVERYFDWIRSGTLNVGFEFAVDRLTAVMLLVVTGVGLLIHIYS
                     AGYMAHEGGYYRFFAYLNLFMFFMLVLVMAANFLLMFVGWEGVGLCSYLLIGFYFLRK
                     SASDAGKKAFIVNRIGDFGFSLAVFLIFITFGSLDFGTVFAAASKMPVESGLGVLTAI
                     GLLLMVGAAGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMVARSAAIYMHA
                     PLALDVVAIVGLATAFFSATIGLAQTDIKKVFAYSTISQLGYMFLGLGAGAFSAGIFH
                     LVTHAFFKALLFLGAGSVIHALSGEQDLRNMGGLRKHTPITFLTILCAAVAIAGVPPF
                     SGFHSKDAILLAAHHHAPWMYWVGVITAGMTAFYVFRAVFLAFFGSYRGHHHPHESPA
                     VITLPLVALALLSLAGGYFDVPRFLEPLFPQAAEEHNFTLVAISVAAGLAGIGLAWLF
                     YVARPGAADAFAASLGRLYQWVYDKYYVDEVYNATIVRPVVGGSTRMLWRGVDVAVID
                     GAVNGIGARARGIGGVLRLFQSGNIRSYAAWVLAGSVLALIAFVFGGSVR"
     gene            10380..11951
                     /locus_tag="VLE22_20860"
     CDS             10380..11951
                     /locus_tag="VLE22_20860"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSB16916.1"
                     /translation="MTFLNLCLLLPLAGFFLLLVIPRARENAIRIACLATSLISFVLS
                     LGLAVSFDAAKSGFQFVTDSPWISSPPIRYHVGIDGLSLWLVLLATLLTPIAALISWR
                     YIEKRVKEFYAFLLLMEFGLIGVFVSLDLILFYVFWEVSLVPMYFLIGVWGHERRIYA
                     AVKFFLYTMAGSVLMLAAMIYLYTLSDTFDFTRLSALMAAGRISLRPAEELLLFLAFF
                     LAFAIKVPLFPLHTWLPDAHVEAPTAGSVILASVMLKMGTYGLLRFCLPLFPSASRES
                     APWVVTLAIVGIIYGALVAMVQPNMKKLVAYSSVSHLGFVVLGIFSFTQLGLDGAVYQ
                     MLNHGVSTGALFMLVGYMYERRHSLEIADYGGVATPAPGLAAVFMITTLASIGLPMLN
                     NFVGEFLVLQGAAQANFKWAVYAAIGVILSACYMLWLYQRAFFGEASETVRSHLRDLT
                     PREWVAIAPLLILMVWMGTATRTFLPAISSANAQILEQTQHNVEFHVRSAPSTEVSVS
                     ALLGTETRTEADHAH"
     gene            11941..13407
                     /locus_tag="VLE22_20865"
     CDS             11941..13407
                     /locus_tag="VLE22_20865"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSB16917.1"
                     /translation="MPTDFVPSAAELCRVLPEIILTLAGTVIMVLTPFSRNRGASRLG
                     SFALAGLLASAVAAIAAQSNPGPAFQGMLMVDGFATFFRLLVIGVGVLTLLSSSHYLR
                     REGVEPGEYYALLLFSVVGQCVMAASNELIIMFVGLEISSIASYILAGYLRDDRRANE
                     SALKYFLLGSFATAFLLYGVAWTYGLTGTTSLPEIRAALTGLESIRSELLVAAAAALM
                     FVGFAFKISAWPFQIWAPDVYQGAPAPVAAFLSAGPKAAAFAILLRVFITAFDPISAR
                     WEPFVWLSALFTMIIGNFAALTQSNIKRLLAYSSIAHAGYVLVAVTAHSQLGTAAAMF
                     YLASYALMNIGAFTVVTHFSRKGERYVDVQDLAGLGVRQPVTAALFSIFLLSLIGVPL
                     TGGFFGKFYVFKAALDGGLVWLTVLGLLNSAVAAYYYLRIIVVMYMHEPGESTDTLPA
                     PGTGLSIALWGSALGTLLLGVYPAVILDFADKSAKFLK"
     gene            complement(13411..14076)
                     /locus_tag="VLE22_20870"
     CDS             complement(13411..14076)
                     /locus_tag="VLE22_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16918.1"
                     /translation="MRDRFAFWLENLRAAGASTKPAGNETMPRGGATPSQLPLRRDLR
                     PSAIWLISTRLPVLGFFVLMFVVFTSVFPPARKWIGLWLGDSGGGTYQAASVPRPTAG
                     GNSVPGKAIFGGSSPAGRLRVGASRTEVIKVLGKPDEERGDVWVYGGARIYFKGDRVT
                     SWSPPGRTQGKRPHNRRADSKRDQSLNPREPRLTEGPVSVPTIEEETLGGTGDMQQTV
                     RRP"
     gene            complement(14269..14478)
                     /locus_tag="VLE22_20875"
     CDS             complement(14269..14478)
                     /locus_tag="VLE22_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16919.1"
                     /translation="MPYVSGKFRTPAPPVMGKGPLDEALPDVKYLKPSLLWRIASHIP
                     LAAAVLIILFLTADHLGLLPAWMRF"
     gene            complement(14693..15379)
                     /locus_tag="VLE22_20880"
     CDS             complement(14693..15379)
                     /locus_tag="VLE22_20880"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSB16920.1"
                     /translation="MTPQHLIVDADDTLWENNVFFERAFEQFVEFLDHSTLDAIGVRA
                     VLDEIELVNNKIHGYGSANFARNLRQCYLRLAERHIREEDLDRVMMFGEKLIHHPMEV
                     IPGVHETLKYLARRHDLTLFTKGHADEQKLKLDRSGLGPYFAHTAIVKEKDADAYREL
                     VKRRGLDPARTWMIGNSPKSDINPALGAGLGAVFIPHAHTWTLERQEIPAGNGRLLIL
                     QSFSELKDCF"
     gene            complement(15376..16014)
                     /locus_tag="VLE22_20885"
     CDS             complement(15376..16014)
                     /locus_tag="VLE22_20885"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSB16921.1"
                     /translation="MQGELLPLFPLNVVLFPRTSLPLHIFEDRYKEMISDTLEEHTEF
                     GVVLAREQGIVNVGCTATVETVYKRYSDGRMDIGTVGRRRFELLLLNEEKSYLRGPVQ
                     FFDDAEEDPAPLPLKQKAFDAFEALRSLEQSGPALKPDLGDPQLSFQLAQIVADLDFR
                     QTLLGLRSENERLRRIVDFLEEFIPRQRYVGRMKSVAPSNGKGQQPGSLLEP"
     gene            16075..16602
                     /locus_tag="VLE22_20890"
     CDS             16075..16602
                     /locus_tag="VLE22_20890"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HSB16922.1"
                     /translation="MILKLKRAPGIYLVGFMACGKSMIGSCLAARLGWGFVDVDADIE
                     SQERASILDIFDTRGEEEFRRLETEALRRRVHMIQAGHPLVVALGGGAFARSVNFDLV
                     EDNGVTVWLDCPLSLIRRRVESSAHRPLARDPVKFEVLYYARRESYARADYCVKVTGD
                     DPEPSVDAILKLPIF"
     gene            16613..17974
                     /locus_tag="VLE22_20895"
     CDS             16613..17974
                     /locus_tag="VLE22_20895"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HSB16923.1"
                     /translation="MSETTLRRNALSIFRAALAASDPVQAVLRHLRLDRDVLITAGRR
                     YRLKSYRRILVVGAGKASAAMALAVERLLGRRVQSGCINVKYGHLAKLRRIGLNECGH
                     PVPDPAGEEGAAHIADMVKSAGEGDLVICLISGGASALLPLPAPPVTLEEKQETTRLL
                     LACGANIHEINAVRKHISLLKGGQLARLAQPATVLSLLLSDVIGDDLDVIGSGPTAPD
                     ESTFRTALSVLRKYGIERDVPAAVRNRIEEGLRGEVPDTPKPGDSTFQRVQNVVVGSN
                     MLAVEAAQRRARELGFRPLVLSTFVEGETREIARMHAAILKEINATGRPVKPPACLIT
                     GGETTVTLRGDGLGGRNQEFVLAAALDLAGLRDVVVLSGGTDGTDGPTDAAGAIADGH
                     TIARANELGLDAARFLSENNSYHFFEPLGDLVKTGPTNTNVMDIRLALAGVPKKQKKR
                     GVL"
     gene            complement(18007..19356)
                     /locus_tag="VLE22_20900"
     CDS             complement(18007..19356)
                     /locus_tag="VLE22_20900"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HSB16924.1"
                     /translation="MKPRSLIGPLLLILIGALFLLHNIRPDLVSFEAPARYWPYILIV
                     WGAIRLFEIVAWWMMNKPVPRSGVSGGEWALVILICLVGAGLSAAHRFGPRLPPMIFR
                     GQSVELFGEAYDFPMAESKTVSGVKRLIIENLRGNTRIVGADVAEIKATGRKTIRAYD
                     KAAADEANRQTPLEIVVRGEEVVVRSNQEKMSGERVVSSDLELTVPHSINIRATGHYG
                     DFDVMNINGSVEIESGNAGVRLQDITGKVRVDLRRSDIIRVINMKGEVDILGRGRDVE
                     LERIDGKVVINGYYSGDLTCRDLKKSFDLQSGGTELSFERLPGQLHMNLGDFSAANVV
                     GPFRLVAKNRDVRLDDFTEAVALTIDRGDVELRPNRGPIAGIEVSTRSGNVELALPGS
                     AKFDLAATAKMGEVTNEYGEPLDVTTEGRGSMIKGAVGGGPKITIETDRGSITVRKE"
     gene            complement(19353..19565)
                     /locus_tag="VLE22_20905"
     CDS             complement(19353..19565)
                     /locus_tag="VLE22_20905"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HSB16925.1"
                     /translation="MNSNEPALFRAIRGPILLIALGALFLVDQFTQYGFWQTWPVLVI
                     LVGTLKLAEHLSARKPRDFETPEGAS"
     gene            complement(19555..20208)
                     /locus_tag="VLE22_20910"
     CDS             complement(19555..20208)
                     /locus_tag="VLE22_20910"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HSB16926.1"
                     /translation="MNCQMHPDRPATAYCRTCGKPLCQECGRLAHGTVFCEEHVPVQA
                     PPQVKPAASQPGTGSPGLAFVLGLIPGVGAIYNGQYVKGLIHVVILGTLISIVSSGGA
                     GGFEPLFGFLIACWFFYMAFEAYHTANKRLRGEVVDEFSSLFPLKPRSGGFPAGPVLI
                     IAIGVLFLLNTLDIVQIGQILRWWPVFLIALGAYMLYNRMAGNSTVPASREEAPHEQ"
     gene            complement(20228..20845)
                     /locus_tag="VLE22_20915"
     CDS             complement(20228..20845)
                     /locus_tag="VLE22_20915"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSB16927.1"
                     /translation="MNCAELDLLLCDYIDGTLEAARKAAVEEHLARCAACAELAKDAA
                     AALAFVERVPAVEPPPELITRILFDISAEREKVGNRRSSLRSMLARLAEPVLQPRFAM
                     GMAMTILSFSMLGRFAGINVRQLQASDLEPAKVWAAVDDRVHRGWARAVKFYESLRLV
                     YEVRSRLRELTEAEMEPANEGAPAEAQQSPEPGPNQTSQQPSKGQ"
     gene            complement(20842..21522)
                     /locus_tag="VLE22_20920"
     CDS             complement(20842..21522)
                     /locus_tag="VLE22_20920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB16928.1"
                     /translation="MAAVAHAAQIPGTWTTDPVLVGWASASAHGCQRVAGNGLDLDTS
                     VVERCLDGEESAWEELTKSHTRRVYSLCYRFTGNSSQAQDLTQEVFLRVFRTLKSFRS
                     GEGSFTTWLTRLTRNLLIDHYRRTRSDRATDSIEEQLPMLEERTALSARTDGLLAGRE
                     ASEVLQAALQKLSPELRETVILRDLEEMEYREIAQTLSVPEGTVKSRLNRGRAELARV
                     LRRQRVAI"
     gene            21697..22758
                     /locus_tag="VLE22_20925"
     CDS             21697..22758
                     /locus_tag="VLE22_20925"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSB16929.1"
                     /translation="MPGGSIQRKPNVRWILSLAALLAAAILLFLFRWRREGFDWRLFA
                     STLQSLDWLWLAIACLVGLATYYGRVLRWAVLIRPVCPRPNMRGLFSATAIGFTAIVI
                     FGRPGEFVRPYLVSLKEKTPFSSQAAAWLLERIFDLLAALLIFGLALAQIGGPGVHTS
                     PTFAWVMKAGGWIAALLGLLCLVVLFLLGRYAESMQQRLLSALSALPARWHSKLVELV
                     AAFVRGVESTRNPGLLLSILLYTSLEWLLIAACYVSLFRAFPDLASLHIADIMIFLGF
                     VSFGSIVQLPGIGGGMQLVAIVVLTELFGASLEVATGMALMLWIITFVVIVPVGVIML
                     FHEGINWQKLREMEDRASI"
     gene            22755..23216
                     /gene="nrdR"
                     /locus_tag="VLE22_20930"
     CDS             22755..23216
                     /gene="nrdR"
                     /locus_tag="VLE22_20930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSB16930.1"
                     /translation="MICPFCGHRQDRVIDSRESKEGDVIRRRRQCLECDRRFTTYERI
                     DEIPYMVVKKDGRREKFDRQKVLSGLLKACEKRPVSMARLAEVVDSVEARLADNPDRE
                     ISTTEIGEMIIERLSALDKIAYVRFASVYRDFQDVEAFLNELKNLMRQKRS"
     gene            23473..25005
                     /gene="rpoD"
                     /locus_tag="VLE22_20935"
     CDS             23473..25005
                     /gene="rpoD"
                     /locus_tag="VLE22_20935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HSB16931.1"
                     /translation="MDQFEEHYLTDSREPLNLPFEEEAKFFDPEETHDVDDFHATQHV
                     EESIYTDDPVRVYLREMGAVPLLTREGEVDLARRMERGKLRMQKAISRSALVQRIVVE
                     LADNMKRAPEELDNWVDLGDVEEGTLAESKRRSEVLQLFSQVTALHKKQQQLADKVAS
                     IAVSNKKLVRRWNGKLSRCKIETSQAIRSIPFHLSRWKYFARDIERVSDELVHLDSEL
                     RRLEGRNGPTTQARARELKREIRKREAAAGVPLVDLRHTLQVIRHGELEAERAKKDLV
                     EANLRLVVSVAKKYVNRGLHLLDLIQEGNIGLMRAADKFEYRRGYKFSTYATWWIRQA
                     ITRAIADQSRTIRIPVHMNESMNKFLRANRELEKELGRAPTNDEISRRMDIPVEKVQK
                     LKTISRDPVSLETPVGRDGESALGDLIEDRWIGSPVDTVIETNVRDETANILKTLSPK
                     EEKVIRLRFGIGCEREHTLEEIGQEFDVTRERIRQIEAKALRQLRSPERARHLRALLA
                     AR"
     gene            25226..26182
                     /locus_tag="VLE22_20940"
     CDS             25226..26182
                     /locus_tag="VLE22_20940"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HSB16932.1"
                     /translation="MLPAQAIPRLARTSPKSVLSHIGDTPLVKLERITSDLPGVEIWA
                     KAEFFNPGGSVKDRAGLNMILDGERTGRLTPDRIILDATSGNTGIAYAMIAAARGYRV
                     KLCLPRNASDERKRMLGAYGAEIVYTDPAEGSDGAIRRCREIYEADPDAYFYPDQYSN
                     PANWKAHFEGTAVEIMNQSEGRLTHFVACMGTSGTFVGTTRRLKRDIPSVKCYSAQPS
                     SGFHGMEGLKHMPTAIVPRIYDPSLADGNLWLETEDAYAMCRRLAREEGLLVGISSGA
                     NVVAARTIGRQLVEAGEPGIIVTILCDGAAKYLSEEFWNDPH"
     gene            26169..26588
                     /locus_tag="VLE22_20945"
     CDS             26169..26588
                     /locus_tag="VLE22_20945"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HSB16933.1"
                     /translation="MIRIESQPWEVMLSHAREAYPDECCGAMLGAVDGDKTVRMALPM
                     ENVSQGPKRSRYELRPEDLLGAEERARQEGMDLIGIYHSHPDCDAYFSETDLKNSCPW
                     YSFVVLSVRGGGFDHANCWLPNADQTGADKEELTHPV"
     gene            26599..26856
                     /locus_tag="VLE22_20950"
                     /pseudo
     CDS             26599..26856
                     /locus_tag="VLE22_20950"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
     gene            26857..28050
                     /gene="moeB"
                     /locus_tag="VLE22_20955"
     CDS             26857..28050
                     /gene="moeB"
                     /locus_tag="VLE22_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HSB16934.1"
                     /translation="MAGGVAVETPAPPATLSKEEVLRYSRHLIMPEVGMDGQLRLKSA
                     SVLLVGTGGLGSPLALYLAAAGVGRIGLVDFDVVDFTNLQRQVIHGTSDVGRKKLDSA
                     AAKMTEINPYVSIGRHEVALSSENALDIIKDYDIVVDGTDNFPTRYLVNDACVLLGKP
                     NVYGSIFRFEGQATVFAYEDGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGLIGLVQA
                     TETVKLILGKGEPLVGRLLLYDALAMRFRELKLRRNPECPACGDHPTITKLIDYHQFC
                     GVPRQEQEPVPSQGLSEWQIDPVQVKAKLDRGDRFLLLDVREPHEYQICRIPAAKLIP
                     LGELPKRIHELDSSEEIVAYCRSGVRSGKAIDFLGQAGFRKVKNMVGGILAWSDKVDP
                     SVPKY"
     gene            complement(28085..31570)
                     /locus_tag="VLE22_20960"
     CDS             complement(28085..31570)
                     /locus_tag="VLE22_20960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB16935.1"
                     /translation="MKTRVLTTFLLVFVCFSAANAQEVRASLTGIVSDPSGAPVAGAT
                     ITVVNLSTNITVSAQTNPTGNYTVPFLAPGTYRMTVQQQGFRRFVRENIVLEAQARVR
                     IDASLELGELSQSVTVSESVSLLQTESATRAQVISNDLLANVPTQGRNPFQIAWTAPG
                     VIKTGSWRYLRSFDIGGTSGLSINGGRNKENEVLLDGISNVQSNRTVIHVPTIETVQE
                     FKVLTNTYDSQYGRTGGGIVTIVTKGGGNEFHGTAFEYFQAEELNANQAELNRTGTRK
                     PPMNINTFGIEGSGPVYVPKVLDGRNRLFWLVSYEGMRQRSADPGVKNFPATEWRGGD
                     FSSRLNAQGAQVLIYDPLTTNAQGERTPFQNNVIPSDRISKVATEVLKYYPAPNAVGD
                     GPARINNYVYPSRWVANMDQWIGRLDLVVNSKNTVYFRYGQNPFSEYRALVFVKDVNE
                     DNPAEPTGNAPLIRNGRNWTWDWTSTISPTMTFNLRAGLARWEETTGNSYGVNYNPTT
                     LGMDAALVGQFSKLQFPRFDLGSYQAVGTDRLINYAANDAYTVQPNLNLVVGRHFLKF
                     GAEGRRYNDNNPNPGLASGAYSFGKNWTQKNANRADATSGDELATFLLGYPTSAYVDR
                     NIDPSYRNHYWAAFLNDDWKVTPKLTVNVGLRWDYEQPRIERYDRMLRGLDFNAASPI
                     ASQVQGLNLKGAVLFAGENGQPRTAFNADKNNFQPRIGASYRIREKWVIRGGYGLYYL
                     GQGEAGSVQGFSRRTTAVVSTDGNLTPAVKMTNPFALQPGGKLLDPIGSSLGEASFLG
                     EGVTANYLDRVLPYSHQYSFDIQRELPGNLLVEAGYSGNITRKLPLNVGLNYVPANEL
                     GRRTADGKIDTAYYTGRVPNPMAGLIENNASLNGATISRQFLMYAFPQFSGVTLQSVP
                     IGQQRYDAFLLKATKRMAHGLTFLTSYTISKTLEQVSLQNAQDFVFADPEATKLEKLL
                     ANELDTPQRFTVAGVWDFPFGKGRPFGGGWPSPIRYALGDWQMNWDIQYMVGWPVGYP
                     NAAQVQPGSANLSGDQRTFAQWFNTSLWNDPATGKRVAAQEAYTLRTFPARFPDVRVP
                     AYNNWDLSLSKYFPIYERVRAQFRFEMVNAFNHPWYMSLASGYNNVTSAAFGQLDPTQ
                     RNLPRFIKLALHMHW"
     gene            31812..32597
                     /locus_tag="VLE22_20965"
     CDS             31812..32597
                     /locus_tag="VLE22_20965"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSB16936.1"
                     /translation="MRKNVPDPMHSSRSRREEAYRHILAKIASGELKAGSTLSEVSLA
                     RNLGSSRTPVREALSQLVAEGLLEQTPNRGAVVVELKRQDIVELYELREALEVYAIGK
                     AARIHVGPDELDPMRRIVKQVLLLKEGLERSDRNVLDFDEMRHLVACDLGYHSLLVRL
                     GGNARMLKVLNETRLLIRIFSIHRSGHDAAQIENICRQHMEILDAVADRDAERAMHIL
                     SAHIRASRQERLDEYDRWQRESSLRSAFPAFLDVPSDLSRSAD"
     gene            complement(32610..33425)
                     /gene="thiD"
                     /locus_tag="VLE22_20970"
     CDS             complement(32610..33425)
                     /gene="thiD"
                     /locus_tag="VLE22_20970"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HSB16937.1"
                     /translation="MNGKTPVALTIAGSDPSGGAGIQADLKTFHQFGVYGEAAITLLT
                     VQNTMRVDRVECMPAELVLQQIRAVVEDIRPGAAKTGALGNREIVEAVAGAAAGFDFP
                     LVVDPVMISKHGAPLIAKEAWWALRKLLLPKAFLLTPNLHEAAALAGIEVADITGMRG
                     AAERLCGLGVKAVLVKGGHLSGSATDVLLFEGKWYEFPGEQIPTRHTHGTGCTYSAAI
                     AATLARGSGLVEAVQLAKQFITEAIRTNPGLGSGSGPVNHHALSTGIHRLPVR"
     gene            complement(33422..34246)
                     /locus_tag="VLE22_20975"
     CDS             complement(33422..34246)
                     /locus_tag="VLE22_20975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB16938.1"
                     /translation="MQTTRPLALITGASSGLGACFAKRLASRGYDLALVARRKHKLDE
                     LAAELRGGSGVNVHTLGADLTKDSERQSVEEYIAGSESLALLTNNAGFGTLGRFHEAG
                     VESQDQMYRLHVLATARLTHAALPGMITRGRGAVINVSSVAAFWQTPFNVSYCSTKAW
                     INAFTESLYLELKAAGSPVRVQALCPGFTYTEFHDVMGVDRKRIPDGFWMSADEVVDA
                     SLRGLDQGHLFVVPGWRYRVIVFLSKVLPRPLRHAAIAGYAKKTGRGHASASASQS"
     gene            34354..35982
                     /locus_tag="VLE22_20980"
     CDS             34354..35982
                     /locus_tag="VLE22_20980"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSB16939.1"
                     /translation="MRLGIDFGTTRIAVAAADRGNYPVVSFECPDGETRDWFPPLVAV
                     RGSEICYGWAAHMSQTGDGWTVVRSLKRLLGRTGLNTPVQIGDRLIHMNRLLGGLTST
                     LRDELRERSNLRLDRGEPLEVMLGVPANSNSNQRFLTAEAFRLAGFSVIGVLNEPSAA
                     SIEFGHKQRLSDQVRERGCVLVYDLGGGTFDASLVEMDEQTHSVLASDGIETLGGDDF
                     DEILAELVFERAGLSEADRDSLRQDELFRLHEECRLKKESIHPNSRRIAVDLDSVRAG
                     WGEVAVPAAEFYEACRPLVQETMNTVENLLASHGLGTNDPRPAVEQYRLEAVYVTGGG
                     SELPLVARELRERFDRRVRRSQHARSATAIGLAIQADVQSGYTLRDRFTRYFGVWREA
                     QGGSTVWFDPLFQKGELLPGPGEWALEVNRRYSPVHNIGHFRYLECSRIGESGEPAGD
                     ITLWDEIRFPFDPVLEQRRDLDSVPVEHTEAARNQSIEEVYTCDAGGALGVTIANLSA
                     DYKRTYRLARWAAREAPVKPARRRPATRRIVSRS"
     gene            complement(36007..36822)
                     /locus_tag="VLE22_20985"
     CDS             complement(36007..36822)
                     /locus_tag="VLE22_20985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSB16940.1"
                     /translation="MLPRVIPFLVLLLAMAAPAQQKSAFRWPDGKRAAVSLSFDDARL
                     SQIDVGMPLFDRIGVKATFYVSPGNLEKRIEGWKKAVAAGHEIANHSRSHPCTGNYGF
                     SKRNALEGYTLERMEEELDGANTIIQSLLGVKPVSFGYPCGLKFVGRGLETKSYVPLV
                     ARGFLSGRGYRDEAANDPEICDMAQLMGTGIDELTFDGMVKHASTALKEGRWVIFAGH
                     EIGKTAFQTTDAAALEQFCRYAKDPANGIWLDTVESIGRYVVKQRSGGGAGGK"
     gene            complement(36827..38224)
                     /locus_tag="VLE22_20990"
     CDS             complement(36827..38224)
                     /locus_tag="VLE22_20990"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HSB16941.1"
                     /translation="MHKYLFPFLVVLIAAPALAQQTVIVRPTEIDDVLLNPGIGFTTF
                     QRFNGDELNQGLKWTEGYPIAYQEYKGSLENKNHPATTIAYFRIYWKFVEPEQGKFNW
                     QMLDTALKTAHSRKQTLWLRIAPYGTTEDNDVPDWYRKMMGDEASRGMIKKWRTDPED
                     PRYAKHFGAMVRAVAARYDGHPDLEAVDLSIVGAWGEGAGSAELTQPTREALVDAYLE
                     GFQKTPLVMLLTDEKTNKYGISKRDVGWRVDCLGDMGGFNNPNWSHMNDYYPEGIINF
                     GMQDAWKKAPVSFEVCWVMQHWLNKGWDVDYIIDQSLKWHISTFNGKSSAVPEKWKPN
                     VDRWLKKMGYRFVLRKFTYPGVVSQNAKVPFTSWWENKGVAPSYTQWPLAIRMKNAQH
                     TMVWRTEADIRNWLPGDNLYDSAFFLPAEMPVGEYDLGIALIDPVSGQAKVKLAIAGI
                     GEDGWYNLGKITVKE"
     gene            complement(38234..39082)
                     /locus_tag="VLE22_20995"
     CDS             complement(38234..39082)
                     /locus_tag="VLE22_20995"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HSB16942.1"
                     /translation="MRQAHLVLIVLASAAVSWAQAKKTVFMVTDAEGVAGICRQEQTD
                     PTDTELRQLLTGEINAAVEGFLAGGADEVIVWDAHDGSRTLSALTIHPKAKLIIGSLG
                     PTGLLERRYSALVFLGQHSMANVRAGIMAHSYSSLGIQNMRMNGKPVGEVETRVALAG
                     WFGVPTILITGDRAAIEELRAIVPDAEVAEVKEGLARYSCISMSAPAARDLIREKARL
                     SMQKIGKVKPYKIDGPVTIEIEYTTRNSLPIDANVRPGAEVVDDRTIRYHGKDFMEAW
                     IRSRMY"
     gene            39128..40027
                     /locus_tag="VLE22_21000"
     CDS             39128..40027
                     /locus_tag="VLE22_21000"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HSB16943.1"
                     /translation="MAPSKAVMFSTKRSQLLVALTMSVIDPIGSRLQAADDAIRSAAT
                     FYASFDSEVKGDFGGGSLKPSTRFNHPTAKGQFVFEPGFNANVFRIAQNKGIRGGALE
                     PMDILPRNGRIFFPARGNIAFREAGWSGSVSFWLKTDPNTMLKTSFCDPLQITQKGAS
                     NGGLWLDFNDAKPKDMRMGSFPAVPEGEKAVSENDPQAPLIRMSTEGFTEDQWRHVVL
                     VWENFDTGRADARTALYVDGKLIGELKDREIAMGWDLDKTGIYVAVSYIGLLDELALF
                     DRPLTPAEITRLREEPGLLAALK"
     gene            complement(40033..>40744)
                     /locus_tag="VLE22_21005"
     CDS             complement(40033..>40744)
                     /locus_tag="VLE22_21005"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HSB16944.1"
                     /translation="ESYNDEAYEKMISYGRLLQSAMGRDWFKYRIDGGYSWEAMEKLQ
                     EVVTLWVCHTVSWDAEKMEHFRKRGVESWFYGPMIYEQRKNSGCGSNTFTDLDLLVNR
                     AIGWIGWKYRSGWVEWEFDWNASAAWYEAENFKEPQRTYNGSGQLIYRGQVMGYQQPI
                     PSIRLKAQRRGLQDHEYFWLLSETTGGKDAADAIVNRIVYKRPFGEAALLDTEIWKNN
                     PDEWEKARIEAGEAIAKR"
BASE COUNT         7443 a        12599 c        12190 g         8512 t
ORIGIN      
        1 tcgtttagca gttctcaaca tgagatggca cttccgtcgg gtaaagaaag gcgaccttca
       61 gattgtactc gaccggcggg ccgtgtcaat aggcgacctg ccgtatttcg ggctaggcgg
      121 tctagtccgg gtggtccacg tttaaggtgg agttaaccgc cgccgacgaa atggacgatc
      181 tcaagctgcg caccggcttc gagcggggtc gccgcccact ccgtctgcct cacgattcgg
      241 ccattcaact caatggccaa gcgcgacggg tcgaggccga gcgagcggag taggccgagg
      301 actgtttgtc ctgtcgcaac tgaccgcgac tgcccgttga caacgaccct gatggtttcc
      361 ggcgctggat ctgccatggc gtgctggtga gtgtcgggat gtgcgatttg acaccccgtt
      421 tcgcctctca ttatattcaa gttttggggc gctgaattac ctagccacga accactatgc
      481 ccacctcgta ccctgaactc tacttcccta tcattgtgca ggctgtgatc gccatggcgg
      541 tcgcggccgg gatggtgggg gcctcgtacc tcttcggcaa gcgcgtgaga aacaaagtga
      601 aagacatgcc gtacgagtgt ggcatcgtcc cgacgggaac ggcgcgggag cgcgtcagcg
      661 tgaagtttta tctcgtggcg atgatcttca tattgtttga cgtcgaagcg atttttctat
      721 acccttgggc cgttgtattc cgggagctga ggctctttgc attcgtcgag atgttcatct
      781 ttgttgcgtt gatcctcgcg ggtttcttct acatctggaa gatgggggct ctcgactggg
      841 gcccagagaa gcggtcgggg gactccgggc gtcatgcttc ctgaaaactt gcgcgagaat
      901 tccgtcgcat ccgcggtggc cgcttcggct ccggatgcca tcgcctcggg caagatcgac
      961 cacggcgaac tcacactgga gatacggccg gaaaagatcg cagccgtctg cgcgatcctc
     1021 aagcaccagc tcagtttcgt ccgtctgagc gatgtcacgg cggtggactg gtatcctcgc
     1081 gaaccgcgct tcgaggtcgt ttaccatcta cactcgctgg agacgaactc gcggcttcgc
     1141 ctgaagtgcc cgctgaacag cgaagctgct gagatcgact ccgtcaccgg cgtctggcgg
     1201 tcggcgaact ggtacgaacg ggaagtgttc gacctgtttg gcatccgctt ccgcaatcat
     1261 cccgatctga agcggattat gatgccggac gactgggaag ggcatccgtt gcgcaaggat
     1321 tacccagtcc acggctaccg ctacgagtac cagaacgaat aggaagagct agtgagcgac
     1381 ccggccacct ccatcgacaa cacgacgccg gccgagaccg gtacgcggcc gcgcagcatg
     1441 gtgctcaaca tgggcccgca gcacccgtcc acgcatgggg tcctgcgggt gctgctcgaa
     1501 ctggacggcg aaaccatctt gaaggccgag tgcgacatcg gctacctgca tacgggtatc
     1561 gagaagcagt gcgaggcgct gacctaccag caggcagtga cgctgacgga ccgcgtggac
     1621 tacctcgcga acctgtccaa caacttgtgc tactgcctcg cggtcgagaa actgctcgga
     1681 gtcgaagcgc ccccgctggc gcagtggatg cgcgtgatgc tcacggaact gacgcgcata
     1741 tgcagtcacc tggtctggct tgggacacac gccttggaca tcggcgcgat gagcatgatg
     1801 ctatactgct tccgggaccg ggaggatctc ctgcgcatct tcgagatgtt ctccggccag
     1861 cggatgatga ccagctactt ccgcgtcggc gggctcgctc tggagcctcc gcggggctgg
     1921 cagcatccag tgaagaagtt tctcgacgca ttcccgagca agatcgacga gtacgaagac
     1981 ctgctcaccg ccaaccccat ctggctgcaa cggacgaagg gtgtggccgt cgtcccgctt
     2041 gacgaactgc tcgatctcgg cgtgaccgga ccgatgctcc gcgccgctgg cctgaactgg
     2101 gacatccgca agacggagcc gtactccggt tacgagaagt ttgacttcga agtgccggtg
     2161 cgcacagaaa gtgacgtcta cgcccggtat caggtccgca tcgaggaaat gcggcagagc
     2221 gtgcgtattt gctggcaggc tatggagggt atgccctccg gttcttgggt ggctgatgct
     2281 ccgaacgtag tgctcccgga gcgggagaag atgaaaaccc agatggaggc cctcatctac
     2341 catttcaaga tcgtcaccga gggattccgc gtgccggcgg gcgaggtata tcagacgatc
     2401 gagtcgccgc gcggggaatt gggctactac gtggtcagcg acggcacgtc caagccgtac
     2461 cgggttcaca tccacggacc aagtttcggc aacctgcagg cgacgccccg aatgatcgaa
     2521 ggcgggctga ttgccgatgt catcgccgcg ctcggtagtc tggatttcat cctcggagac
     2581 gtggaccgat gactttctcg ccggatatgg aggcaaagtt cgagaagctt gtgcggagct
     2641 atcctcccgg gcggcaacgc tcggcgctga ttccgatgct actctacgcg caggacgaga
     2701 ttggctcggt gacgccggag cttgttgacg aggtggcgcg caggcttggg atcacgccgg
     2761 tcgaagtcga cggcgtcgtc acgtactact ccatgcttcg caagaagccg atggggaaat
     2821 accacctcca gatctgcacg aacatcagtt gcatgctcac gggcggaacg gagctctggg
     2881 aacacgcttg ccgcaaactc ggtatcggca acaaagaggt gacccccgat gggcagttct
     2941 cactcgaaga ggtggagtgc atcggcgcct gttcctgggc gccggcaatt caggtgaact
     3001 acgattttca gcaccatctg actcctgaga agctggaccg gctcatcgag agcctgcgaa
     3061 aggcgcccca ataaccatgc tgtacaacga gcccagcccg ctcgaagtca aggtgcttac
     3121 gcggcgcttc ggcctgccgg attccgcttc tctcgacacg tatctggcga gcgacggcta
     3181 cgctgccctc cgcaaggcgc tcggaatgaa gcccgaggag atcctcgagc agatgaaacg
     3241 ctccgtgctc cgcgggcgcg ggggcgccgg ttttccggcg gcaatgaagt ggggtttcgt
     3301 accgcgcagt tcgcccaaac cgaagtacat cgtggcgaac gcggacgaga gcgagcccgg
     3361 cacgtgcaag gaccggctgc tgatggaaaa cgatccccat cagctcatcg agggaatgat
     3421 gatcgccggg ttggtagtgg acgctcacgc cggctacatc tacatccggg gtgaataccg
     3481 gtacctgatc gaaatccttg accgagcgat cgctgaagcg tacgagcgcg gctatctggg
     3541 caagaacatt ctcggcagcg ggttcgattt cgatctctac acgcacaccg gggccggggc
     3601 ctacgagtgc ggcgaggagt ccgcgttgct ggagtccctg gaaggcaagc gcggaattcc
     3661 tcggatccgc cctccgttcc cagccgtggt tggcgcgttc caatgcccca cggttctgaa
     3721 taacgtagag accctttcgg cggtgccggc gatcattcgc gacggagggg tggccttttc
     3781 cgctctcgga acacctaaga atggagggac caggctcttc tgcatctccg ggcacgtgaa
     3841 caaaccgggc gtctacgagc tgcccatggg cttcaatctc ctgcggatga tcgacgaagt
     3901 cgcgggcgga gtgcgcgacg gcaggaagct gaaagccgtg atcccgggcg gctcgtcctg
     3961 cccggttctc aaggcggagg agtgcgatat tgccatggac ttcgactctc tgtcgaaggc
     4021 gaaaagtatg ctcgggtcgg gcggcgttgt cgtcatggat gaagcaacgt gcatggtgaa
     4081 agccgctcta cgcatcatgc gcttctacgc ccacgagagt tgcggctggt gcattccctg
     4141 ccgggaaggt acgctctggc tgagaaagat ccttacacgt ttccacgagg ggcaggggcg
     4201 gcccagcgac attccgttga tcggcgaact ggcgaagaat atgctcggcc ggacattctg
     4261 cccgctcggt gatgccgccg ccatgcccac gatctcgttc gtcgaaaagt ttcgggacga
     4321 gttcgaggcg catctggacg gcaagccctg ccccttcgaa gaagcggacc ggacggtgct
     4381 cgcgtaatgg cagatctcgt cagactcgaa atcgacggca ggatggtcga ggtgcctcca
     4441 ggcacgctcg tgatcaacgc ggccaagcag gcgggcattg agatcccggc gttctgttac
     4501 tacgaggggc tcgcgctcca ggccgcctgc cgcatgtgtc ttgtcgaagt cgagaggatg
     4561 ccaaagctga tcgcctcgtg cacgcttcct gtctccgaag gcatggtggt tcgtacagaa
     4621 actccgcagg tagttgaggc ccggaaatcc atgctggagt ttctgttgac caaccacccg
     4681 ctcgactgcc ctgtctgcga caagggcggg gagtgcgagc tgcaggatat ggccttccgc
     4741 tacggcaccg gcgagagccg gttcaccgaa gtgaagcacc atgtcgagga gcagcagtgg
     4801 tctccggttg tgttcttcga tgcgccgcgg tgcatcctat gctaccgctg cgtgcgaatc
     4861 tgctccgagg gcatgggggt tagcgccctt ggcgtgatca accgcggggc atcggccgag
     4921 atcgcgccca attgcgacga tcacctcgag tgcgacgagt gcggcatgtg catcgacatc
     4981 tgcccggtcg gcgctctcac cagcggcatg taccgctaca agacccggcc ctgggagatg
     5041 aagcacgagg gtaccgtttg cgcacattgt tcgaatggct gcaagacgac cttaggcgtg
     5101 tacgacaacc gcattatgcg ggggaacaac cgggaccgct cgggaatcaa cggggagttc
     5161 ctctgcatca agggccgcta cgcgttcgac ttcgtcagcc accctgagcg actccagtct
     5221 cctctgatga agattgatgg ccggctccag gaggtccctt ggtctcaggc gctgtccgcc
     5281 gtcgccggcc ggttctcgga gatcaaggcc cgtggtggca aaatcggcgt gatcggctcg
     5341 actcgcacga caaacgagga gaattactat cttcagaagt tcgcccgcgt cgggctcggc
     5401 acaaatcaca tcgaccacca ccggtccgga gatgtcgcca ctctgctcac tgctctcggc
     5461 ggccgctccg atgccctggc gactaccgac gacctgtacg ggcgcaaggc attcctgatc
     5521 gtcgccgccg acctcgcgca gcagcacccg ttcctcgcct tccagatccg tgccaactgg
     5581 cgtcatcaca actgccggac ttacacggtc acgccggggc cggtccggga ggactcttac
     5641 gccgtgcgca gtgtccaggt ccctgagggt gaagaactcg acgctgtgga ggcgctgcgc
     5701 gacagcctcg cggccgaacc ggaactggtc gtactcttcg gcgacacgat caagtgcgac
     5761 gcagtgcggc ggctggtcgc gttcggcgat tcgctcggga tcccggtcaa gtacgtctgt
     5821 ctcgtcgatt actcgaattc gcgcggtgcg atggacatgg gtttgctgcc ggacctgttg
     5881 ccgggctacc gtcccgtgac cgaggcggga cttgagcccg gtatgacatt gcccgaaata
     5941 ctcgcggctc ccgacttggc ggggctctgg gtggttggcg ctaatccctt gacgaacaag
     6001 cagttggttt cggatgcagc ctttgtggtg gtgcaggatc tgttcctcac cgaaaccgca
     6061 caacgggccg acgttgtgtt tccggcggcg tccgcctacg agaagtccgg caccgtgacg
     6121 aacgtgtgcg gcgaagtcca gcggctgaaa agagccgtct cggtcacggg cgcgaaacct
     6181 gaccttgaga tcttcggcct gctggcaaag gaaatgaagc tcgacctcgg aatctggact
     6241 cccgacgcgg ttttcaaaga gattcgcgac gcggtccgcg ggtataatgt ccctttgccc
     6301 gtgatcgtca cgggcggggc atcgcagaca gctccggagg gcgggccggc tggggccaca
     6361 tcgcggccgg atctgatcca gtcggcgcgg gatacgcttt tcacttccgg gtcgctaagt
     6421 cggttttcga agacgctgaa ttccgttacg gagcggcctg gggcgctgta ccgcggttga
     6481 gagccggcgc cgaagactgc accacgaaag tttcacgcga atgagttttc tcagcctgag
     6541 cctgctgaag gccacggtag tggcagcggt gttgctcacc acgcttgcct acttgcagtg
     6601 ggtggagcgc aaggtgatcg cccacatcca attgcgcctc ggcccttcca gggtcgggcc
     6661 ccacggtctt ctccagccgg tggccgacgt gatcaaactc gccaccaagg aaggtttcat
     6721 cccgccccac gccaacaagt tcctgtacct gttcgcaccg ttcctggcag tgacactggc
     6781 gctactctcc atctctgtga ttccgttcgg tcccgagatc gagattttcg gtatccggac
     6841 cgagatgcag ctcaccaatc tgaacatcgg cgtcctcttc ctgctcgcgg tttcttcgat
     6901 gagcgtgtac agcgtcgctc tcgccggctg ggcgtcgaac aacaagtacg cgcttctcgg
     6961 aggtctgcgc agcgcggctc agatgatcag ttacgaactg ccgctggcgg tggcggttgt
     7021 atccgtactg ctgctcgcca atacgctgag tctccgtggg ctggtcgagt cccaaagtgg
     7081 cttctatatc gggttcattc cccgctggaa cgttttccag ttgcccttcc cgcaagtatt
     7141 cgcattcctg atcttcctgg tcgccgcctt cgccgagacg aaccgcatcc cgttcgacat
     7201 gccggaggcc gaaaacgaac tggtggccgg cttccatacc gagtacagca gtctgaaatt
     7261 tgccgcattc ttcatggcgg aatattccaa catgatcacg ggaacctgta tggcgacgct
     7321 cctcttcctg gggggctgga atccaccggt accgaccggg ttgggcgcca attacttccc
     7381 gtctttagtc tttgccgctg gcggcgccgt ggccctctac cacggcttgc acccggcccg
     7441 cagccgggac cgctacagcc ttccggttgt ggctctggtc tttttcctgg ttgcggccat
     7501 gctgctgatt ccagtggtgc agggaattct ggctccggtc ttctggttcg gagccaaagt
     7561 cggttttctg ctcttcgcgt tcatctgggt gcgcggcacg ttgccacgtt ttcggtatga
     7621 ccagttgatg cgcttcgcgt ggaagtttct gttcccagtc gccgtgctga atctgctggt
     7681 tacgggctta ctggtggcgg tggtctcgaa atagcgatgg acgtcattct ctttttggtc
     7741 ttcgccggga tcgcagtcgt ctgcgcggtc aatctggtgg tacagaccca cccgatcgcc
     7801 agtgcaatct cccttgtcgg cgtcatgggc tcgctggcgg tgttatacct tctgctcgga
     7861 ggcgaattca tcgccgccgt tcaggtgatc gtctacgccg gcgccatcat ggtgttgttc
     7921 gtcttcgtca tcatgctgct gaacgccggc gccgagcaga aatccggagg gtcgctaacg
     7981 gcccgttatc tcggcatccc tctcctgatc gtgctgctcg ggttgctgag tttcctgatt
     8041 cgcatcatgt tgccgccgtc ggaaggggtc aggttcgggg ggttcacggg aggctctccg
     8101 gaagcggttg ggcgggtgct gtttacgcgt tatctcctgc cgttcgaagt gacgtcggtc
     8161 ctcatcctag tcgctatcct cggcgcgatt gtgcttgcac ggaaggagct ggattgaata
     8221 tgaccgccgt accgctgtcg tggtacctga cgctgagcgc catcctgttc gcactcggcg
     8281 tggccggctt cctgatccgc cgaaacatca ttaccgtttt catgtccatc gagttgatgc
     8341 tgaacgcggt gaacctcagt ttcgtcgcct tttcgtacca gctcaagcag gtggacggac
     8401 atatcttcac gttcttcgtc atggtggtgg ccgccgccga ggcggctgtc ggtctcgcga
     8461 ttatcctcac ggtgtttaag aatcgcggca cgttgcagat tgacgaagtc agctcgatga
     8521 agtactaaca cgatgctgca cctctggctc atccccgctc tgccgctcgc cggtttcctc
     8581 gtgaacggcc tgttcggtcg ccgcatgccg aaggcgctgg tcgatgccgt cgcgatcggc
     8641 tccgtgctgc tttcatttct ctgggtgctc cggacactct ccgctctggg gacgatggag
     8701 gccgcctatg tcgagcgcta ctttgactgg attcgcagcg gcacgctgaa cgtcggcttc
     8761 gagttcgccg tcgaccgcct caccgccgtc atgctgcttg tcgtcaccgg cgtcggcctg
     8821 ctcatccaca tctattcggc cggctacatg gcccacgagg gcggctacta ccgctttttc
     8881 gcttacctga acctgttcat gttcttcatg ctggttctcg tgatggcagc caacttcctg
     8941 ctgatgtttg tcggctggga gggcgtcggg ctgtgcagtt acctcctgat cggcttctac
     9001 ttcctccgca agagcgcctc cgacgccggc aagaaggcgt tcatcgtgaa ccgcatcggt
     9061 gatttcggct tctcgctggc cgtctttctg attttcataa ccttcggttc gctggatttt
     9121 ggcacggtct tcgcggccgc atcgaaaatg ccggttgagt ccggcttggg tgtcctgacg
     9181 gcaatcggcc tgctcctgat ggtcggcgcc gccggcaaat ccgcccagat tcccctctac
     9241 gtctggcttc ccgatgcgat ggagggcccc acgccggtct ccgcactgat tcacgcggca
     9301 acaatggtca cggcgggcgt ctacatggtc gcccgcagcg cggcgatcta catgcacgct
     9361 ccgctcgcgc tggacgtggt cgcaatcgtg ggtctcgcga ccgcgttctt ctcggcgacg
     9421 atcggtctcg cacaaaccga catcaagaag gtcttcgcct actcgactat ctcgcagctc
     9481 ggatatatgt tcctcggact gggtgcgggc gccttctcgg ccggtatctt ccacctggtg
     9541 acacacgcgt tcttcaaggc cctgctcttt ctgggcgcgg ggagcgtgat tcacgcactc
     9601 agcggggagc aggacctccg gaacatgggc ggccttcgca aacacacgcc gatcactttc
     9661 ttaacaatcc tctgcgccgc tgtcgccatc gccggggtgc cgccgttctc ggggttccac
     9721 agtaaagacg ccatcctcct ggcggcacat caccacgctc cgtggatgta ctgggtgggg
     9781 gtgatcacgg ccggcatgac cgcgttttac gtcttccgag ccgtgttcct cgccttcttc
     9841 ggcagttatc gagggcatca ccatccgcat gagtcgcccg cggtgatcac tttgccgctc
     9901 gtggcgctcg cgcttctgtc gctcgccgga gggtacttcg acgtgccgag gttcctcgaa
     9961 cctctattcc cccaggcagc ggaggaacac aacttcactc tggtggccat atccgtcgcc
    10021 gcgggactgg caggtattgg gctcgcctgg ctgttctatg tggcgcggcc cggcgccgcc
    10081 gacgcattcg cagcgagcct cgggcgcctg tatcaatggg tctatgacaa gtactacgtc
    10141 gacgaggtgt acaacgcgac gatcgtccgt ccggttgtgg gaggctcgac acggatgctc
    10201 tggcgcggag ttgacgtcgc ggtgattgac ggtgcggtaa acggtatcgg cgcacgagcg
    10261 cgcggaatcg gcggtgtcct gaggctcttc cagtccggga acattcgaag ctacgccgcc
    10321 tgggtgttgg ccggatcggt ccttgctctc atcgcgttcg tattcggggg aagtgtccga
    10381 tgaccttctt gaacctctgc ctcctccttc ccctcgccgg gttcttcctg ctgctcgtca
    10441 ttccccgggc tcgcgagaat gccattcgga tcgcctgtct tgcgacctcc cttatctcat
    10501 tcgtgctgtc gctaggcctg gcggtgagct ttgacgcggc gaagtccggt tttcagtttg
    10561 taacggactc gccgtggatt tcatctccgc ccatccgcta tcacgtcggc attgacggcc
    10621 tcagcctctg gctggtccta ttggccacgc tgctgactcc gatcgccgcg ctgatttcct
    10681 ggcgctacat cgaaaagcgc gtgaaggagt tctatgcctt cctgctgctc atggagttcg
    10741 gactgatcgg cgttttcgtc tccctggatc tgatcctgtt ctatgttttc tgggaggtct
    10801 cgctggtgcc gatgtacttc ctcatcggcg tctggggcca cgaacggcgg atttacgctg
    10861 ccgtcaagtt cttcctttac accatggccg ggtcggtgtt gatgctggcg gccatgatct
    10921 atctctacac actctcggac accttcgact tcaccaggct ctcggcgttg atggcggcgg
    10981 gccggatctc cctgcgccca gccgaggagt tgctgttgtt cctcgcgttt ttccttgcct
    11041 tcgcgatcaa ggttccgctg ttcccgctcc acacctggtt gccggacgcg cacgtggagg
    11101 cgcccacggc tggttctgtc atcctcgcct cggtgatgct gaagatggga acctatggac
    11161 tgctgcgatt ttgcctgccg ctgtttccct cggcatcgag ggagagcgcg ccctgggtgg
    11221 tcacgctggc gatcgtcggc atcatctacg gggcccttgt ggcgatggtc cagccgaaca
    11281 tgaagaagct tgtggcctat tcgtccgtga gccatctggg cttcgtcgtg ctcggcattt
    11341 tctcgttcac ccaactcggt cttgatggcg ccgtctacca gatgctgaac cacggcgtgt
    11401 cgaccggagc gctcttcatg ctggtcgggt acatgtacga acgccggcat tcgctcgaga
    11461 tcgccgacta cggcggggtc gcgactcccg ctcccggact ggccgccgtt ttcatgatca
    11521 ccactctggc aagcatcgga ttgccgatgc tcaacaattt cgtcggcgaa ttcctcgtcc
    11581 ttcagggtgc cgcgcaggcg aacttcaagt gggctgtcta cgccgcgatc ggggtaatcc
    11641 tttcggcctg ttacatgctc tggctttacc agcgcgcctt cttcggcgag gccagcgaaa
    11701 ctgtccggtc ccatctccgg gatctgaccc cgcgcgaatg ggtggccata gcgccgctac
    11761 tgattttgat ggtctggatg ggaacggcta cccgcacctt tctgcccgca atcagctccg
    11821 cgaatgcgca aatcctcgag cagacgcagc ataacgtaga gtttcacgtc cgatcggctc
    11881 cttcgacgga ggtgtcggtt tcagccctgc tcggaactga gactcgcacg gaggccgacc
    11941 atgcccactg attttgtccc atcggccgcc gaactttgcc gggtccttcc cgagatcatt
    12001 ctcaccctcg ccggcacggt gatcatggtt ctgacgccgt tctcccggaa cagaggtgct
    12061 tcgcggctcg gttccttcgc gctcgccggc ctgctggcgt cggccgttgc ggcgattgcg
    12121 gcacagtcga acccgggccc cgcatttcag gggatgttga tggtcgacgg cttcgcgacg
    12181 ttttttcgac tgcttgtcat tggcgttggc gtccttacgt tgctttcctc cagtcactac
    12241 ctccgccgcg aaggcgttga gccgggcgag tactacgcac tgcttctgtt ctcggtggtc
    12301 gggcagtgcg tgatggcggc ttcgaacgaa ctgatcatca tgttcgtcgg tctcgagatc
    12361 tcgtcgatcg ccagctacat cctcgccggc taccttcgcg acgaccgccg cgccaatgag
    12421 tcggcgctca agtatttcct gctcggctcc ttcgccacgg cgttcctgct ttacggcgtc
    12481 gcgtggacct acggattaac gggcacgacc agcctgcctg aaattcgggc cgcgctcacc
    12541 ggacttgagt cgatcaggtc cgagctcctt gtcgccgccg ccgccgcgct gatgttcgtc
    12601 ggcttcgcgt tcaagatttc ggcatggccg tttcagatct gggctcccga cgtctatcag
    12661 ggagcgccgg cgcctgtcgc cgcgttcctc tccgcgggtc ccaaggccgc cgccttcgcc
    12721 atcctgcttc gggttttcat cacggccttc gatccgatca gcgcgcgttg ggagcccttt
    12781 gtatggctct cggctctgtt tacgatgatc atcggcaact tcgccgcatt gacgcagtcc
    12841 aacatcaagc ggctgctcgc ctacagttcg atcgctcacg ccggctatgt tctggttgcc
    12901 gttacggcgc actctcagtt gggtaccgcg gccgcgatgt tctacctcgc ctcttacgca
    12961 ctgatgaaca tcggcgcctt caccgtcgtg acccacttct cccgcaaggg tgagcgttac
    13021 gtcgacgtgc aggaccttgc cggtcttggg gtgcgccaac ccgtcaccgc cgcgctgttc
    13081 tcgatttttc tgctctcgtt gatcggcgtc cccctcaccg gcggcttctt cggaaagttt
    13141 tatgttttca aggctgccct cgacgggggc ttggtctggc tgaccgtgct cggtttgttg
    13201 aatagcgccg ttgccgccta ctactatctg cggatcatcg tcgtgatgta catgcacgag
    13261 cccggcgagt cgacggacac gctgcctgcg cccggtacgg gtctcagcat tgctctctgg
    13321 ggttctgcgt tgggcacgct gctgctgggc gtttacccgg cggtgatcct ggacttcgcc
    13381 gacaagtccg cgaagtttct gaagtaggaa ctacggcctc cggacggtct gctgcatgtc
    13441 gcccgtcccg ccaagagtct cctcttcaat ggtggggacg ctgaccggcc cttccgtcaa
    13501 tcgaggctcc cggggattga gcgactggtc ccgctttgag tccgctctcc ggttgtgggg
    13561 gcgcttgcct tgtgttcggc cgggcggcga ccaggatgtt acgcgatcgc ccttgaagta
    13621 gatcctcgcg ccgccgtaaa cccaaacgtc gcctcgctct tcgtcgggct tgccgaggac
    13681 cttgatcact tccgtgcggc tcgcgcctac cctgagacgg cccgccgggg acgatcctcc
    13741 gaaaatcgcc ttgccgggga cagagttacc accggcggtg ggtcgcggta cgctcgccgc
    13801 ctgatacgtg ccgccacccg agtccccgag ccataggccg atccatttcc tggctggcgg
    13861 aaacacactg gtgaagacca cgaacatcag tacgaagaac cccaagacag gcagccgtgt
    13921 ggagatcaac cagatagccg acggccgcag gtcccggcgc aatggaagtt gtgatggagt
    13981 cgcgccaccg cgcggcatcg tttcgttccc tgcgggtttc gtcgaagctc cggcggcgcg
    14041 cagattctca agccagaatg cgaaccgatc cctcatcgct gccctcacat cacagccttg
    14101 tatcctggct ctcttcttgg gggaccaact aatggtcgtc tgtcaggggt tggtctgtaa
    14161 atagcactac cccaggacta acgccccgtg gtaccaagac gaaccacgcg cccgtcctag
    14221 taccactcct cacgcgggcc gaccgttacc tgacggagcg ctcggcgctc agaaccgcat
    14281 ccaggctggc agcaatccga ggtggtcggc ggtgaggaaa aggatgatca agactgcggc
    14341 agccaggggg atgtggctgg ctattctcca aaggagcgag ggtttgagat acttgacgtc
    14401 cggcaaggcc tcgtcaagag gtcctttccc catgacgggg ggagccgggg ttctgaactt
    14461 ccccgatacg tatggcatag gctcccttcc ggcgcctcgt gcacgctcgg cacgcagggc
    14521 gccagcactg gacattctaa ctcggttgcc cactttcagg aaatagcgaa aaagtcacac
    14581 tggttatcgt cgtaccggta cgattcatta cgattttcgc tgggctgccg ctcccggacg
    14641 gccgcggctc cttaagagca tgagcgatgt tcggactttt tcagcactcc tgttagaagc
    14701 aatccttcag ttccgagaag ctctgcagga tcaggagccg gccgttgcca gccggaatct
    14761 cctgccgctc gagcgtccag gtgtgcgcgt ggggtataaa caccgctcct agtcccgcgc
    14821 ccagtgcggg gttaatgtcg gatttcggcg agttccctat catccacgtg cgtgcgggat
    14881 cgagaccgcg ccgcttcacc agttcccggt atgcgtccgc atccttctcc ttcacgatcg
    14941 ctgtgtgcgc aaagtacggt ccgagtccgg agcgatccag cttgagtttc tgctcgtccg
    15001 cgtgaccctt tgtgaacagc gtcagatcgt ggcgccgagc gaggtacttg agcgtctcgt
    15061 gcaccccagg gatcacctcc atcgggtgat gaatcagctt ctccccgaac atcatcacgc
    15121 ggtccagatc ttcctcacgg atatgcctct cggccagacg cagatagcat tggcgaagat
    15181 tgcgcgcgaa atttgccgaa ccgtagccgt gaatcttgtt gttcaccagt tcgatttcgt
    15241 ccagcaccgc ccgaaccccg attgcgtcga gtgtcgagtg gtccaggaac tcaacgaact
    15301 gttcgaacgc ccgctcgaag aaaacgttat tctcccagag tgtgtcgtcg gcgtcgacga
    15361 tcagatgttg cggggtcatg gttccaagag gcttccgggc tgctgcccct tgccgttgct
    15421 cggagccacg gacttcatgc gtccgacgta ccgctgccgg ggaatgaact cttcgagaaa
    15481 atccacgatc cggcgcagtc tctcattctc cgatcgcaat ccgaggagag tttgccggaa
    15541 gtcgaggtct gcgactatct gggcgagttg gaagctgagc tgtggatcac ccagatcggg
    15601 ctttagagcc ggcccagatt gctccagtga acgcagtgcc tcaaaggcgt caaaggcctt
    15661 ctgcttcagc gggagaggtg cggggtcctc ctccgcgtca tcaaaaaact gaaccggccc
    15721 gcgcaagtag ctcttttcct cgttcagaag aagcagttca aagcgcctcc gcccgacggt
    15781 accgatatcc atcctgccgt ccgaataccg tttgtagacc gtctccacgg tggcggtgca
    15841 cccgacattg acaatgccct gctcccgcgc gagaaccacg ccgaattccg tatgttcctc
    15901 cagggtgtcg ctgatcattt ccttgtatcg atcctcgaaa atgtgcaggg gcaaggaggt
    15961 tcggggaaac agcactacat tcaatggaaa gagcggcaac aattccccct gcatgctacc
    16021 attatggctg ccgtacgccc aatgggtcga gtccccgggg ctggagcgga gtggatgatc
    16081 ctcaagctaa agcgcgcacc cggcatctac ctcgtcggtt tcatggcttg cgggaaaagc
    16141 atgatcggtt cctgcctcgc ggcacgtctc ggttgggggt tcgtggacgt tgacgccgat
    16201 atcgagagtc aggaacgggc gtcaatcctt gacatcttcg acacgcgcgg cgaggaggaa
    16261 ttccgcaggc tggaaaccga agccttgcgc cggcgcgtcc acatgatcca ggctggtcac
    16321 ccactggttg tcgcgctcgg aggcggcgcg ttcgcacggt cggtaaattt cgacctcgtg
    16381 gaagacaacg gcgtgacagt gtggcttgac tgcccgcttt cgctgattcg ccggcgcgtc
    16441 gagtcatccg cgcaccgtcc cctcgcccgc gatccggtca agttcgaagt tttgtactac
    16501 gcccggcgcg aaagctatgc ccgtgcggac tactgcgtca aagtaaccgg cgacgatccc
    16561 gaacccagcg tcgacgcgat cctcaagctg cccatattct gagatgtccc caatgtccga
    16621 aaccacgctg cgccgtaacg ctctttccat ctttcgagcc gcccttgctg cctccgatcc
    16681 ggtccaggct gttctccgtc acctgagact ggaccgtgat gtgctcatta ctgcgggccg
    16741 ccgctaccgg ttgaagtcat accggcgaat cctggtagtg ggggccggca aggcgagcgc
    16801 cgctatggcc ctggctgtcg agcgccttct tgggcgccgc gtccagtccg gttgcattaa
    16861 cgtgaagtac gggcaccttg cgaaactcag gcgtattggg ctgaacgagt gcggacaccc
    16921 cgtgcccgat cccgccggcg aggaaggtgc cgcgcacatt gcggacatgg tgaagagcgc
    16981 cggggaaggt gacctcgtga tctgcctgat ctccgggggc gcttccgcac tgctccctct
    17041 cccggctcct ccggttaccc tcgaggaaaa gcaggagacg acgcggctcc tgctcgcctg
    17101 tggcgcgaat attcacgaga tcaacgcggt tcgcaagcat atctccctac ttaagggggg
    17161 ccaacttgcc cggctggcgc agccggcgac cgtcttgtca ctcttgcttt ccgacgttat
    17221 cggagacgac ctcgacgtca tcggctctgg tcccaccgca ccggatgagt cgacgtttag
    17281 gaccgccctg tcggttctgc gcaagtacgg catcgagcgg gatgtcccgg cggccgtgcg
    17341 aaaccgcatc gaagagggat tgcgcgggga ggttcccgac acaccgaaac caggcgactc
    17401 gacattccag agagttcaga acgtcgtcgt tggcagtaat atgctcgcgg tagaggccgc
    17461 ccaacggcgt gcccgcgaac ttggctttcg tcccctggtg ctgtccacat tcgttgaggg
    17521 ggagactcgc gagatcgccc gcatgcatgc cgcgatcctc aaggaaatca acgcgaccgg
    17581 acgccccgtg aagccgcctg cctgcttgat cacgggaggt gagacgacgg ttactctgcg
    17641 aggtgacgga cttggaggcc gcaaccagga gtttgtcctc gcggctgccc tcgatctcgc
    17701 cggattgagg gatgtcgtcg tgctgagcgg cgggaccgac ggcacggacg gtcccaccga
    17761 cgccgccggc gcaatcgcgg acggccacac catcgcgcgg gcgaacgaac tcggtcttga
    17821 cgccgcccgg tttctatcgg agaacaattc ctaccatttc tttgagccct tgggcgactt
    17881 ggtgaagacg ggcccgacga acacgaacgt gatggacatc cgtctcgcgc tcgccggcgt
    17941 gcccaagaaa cagaaaaagc gcggagtgct ctgaatctga ggcactccgc gccccggcgt
    18001 tggattttat tcctttcgta cggtgattga accccggtcc gtctcgattg tgatcttcgg
    18061 cccgccgccg accgcgccct taatcatcga ccctcgtccc tcggtcgtca cgtcgagcgg
    18121 ttcgccatac tcgttggtca cttcgcccat cttcgccgtc gcagccaggt cgaacttcgc
    18181 cgaacccgga agagccagtt ccacgttgcc cgaacgggtc gaaacttcaa ttcccgcgat
    18241 cggaccacga tttggccgga gctctacgtc gccgcggtcg atcgtcagcg caaccgcctc
    18301 ggtgaagtcg tcgagccgca catcgcggtt cttcgccacg agccgaaacg gcccgacaac
    18361 gttagcagcg gagaaatcgc caaggttcat gtgcaactga cccggcaatc tctcgaaact
    18421 gagttccgtc cctccgctct gtagatcaaa ggacttctta aggtccctgc aggtgaggtc
    18481 acccgaatag tagccgttga tcacaacctt gccgtcgatc ctttccagtt ccacgtccct
    18541 gcctctgccg agaatgtcga cctcaccctt catgttgatg acgcgaatga tgtcgctgcg
    18601 ccgcaggtcg acgcggactt tgcccgtgat gtcctgaagt cttactccag cgttgccgct
    18661 ctcgatctcg acgctgccgt tgatgttcat tacgtcaaaa tcgccgtagt ggccggtcgc
    18721 ccgaatgttg atgctgtgcg gaacggtcag ttccagatcc gacgacacca cccgctctcc
    18781 ggacatcttc tcctgattcg accggaccac gacttcttcg ccgcggacga cgatctcgag
    18841 cggcgtctgg cggttcgcct cgtcggccgc cgccttgtca tacgcgcgga tcgtcttgcg
    18901 tcccgttgcc ttgatttctg ccacgtcggc gcccacaatc cgcgtgttgc cccggaggtt
    18961 ttcgatgatc aagcgcttta cgccggaaac tgtcttcgac tcggccatcg ggaagtcgta
    19021 cgcttcgcca aagagttcga cgctctggcc tcggaagatc atcggtggga gtcttggtcc
    19081 aaagcgatgc gccgccgaga gccccgctcc gaccaggcag atgagaatga ccagcgccca
    19141 ttcgccgcct gaaacaccac tcctggggac cggcttgttc atcatccacc acgccacgat
    19201 ctcgaacagg cggatcgcac cccaaacgat caatatataa ggccagtagc gcgccggcgc
    19261 ctcgaacgaa accaggtcgg gccgaatatt atgcagcagg aacagcgcgc cgatcaggat
    19321 cagcagcagt ggaccgatca atgacctcgg tttcatgacg caccctccgg ggtctcgaag
    19381 tcgcgaggct tgcgtgctga caggtgttcc gccagtttca aggtcccgac aaggatcacc
    19441 agcaccggcc acgtctgcca aaacccgtac tgggtgaact ggtccaccag aaatagcgcg
    19501 ccgagagcaa tcagtaaaat cggaccgcgt atcgcgcgga ataatgcggg ctcgttactg
    19561 ttcatggggc gcctcctcgc ggctcgctgg aaccgtcgaa ttcccggcca tccggttgta
    19621 cagcatgtac gccccaaggg cgatcaggaa gaccggccac caccgcagta tctggccgat
    19681 ctgaacgata tccagcgtgt tcagcaggaa cagcaccccg atcgcaatga tgagcacagg
    19741 tccggcgggg aagccgccgg agcggggctt caaaggaaag aggctggaga attcgtctac
    19801 cacttccccc cggaggcgct tgttcgccgt gtgatacgcc tcaaacgcca tgtagaagaa
    19861 ccagcaggcg atcaggaaac caaacaatgg ctcaaaccca ccggcccctc ccgaacttac
    19921 aatactgatg agcgtcccaa gaatgacgac atggatcagg ccctttacgt actggccgtt
    19981 gtagatggcg cccacccccg ggatcagccc gaggacaaac gccaggccgg gtgatccggt
    20041 cccgggttga ctggccgccg gtttcacttg cgggggcgct tgcaccggaa cgtgttcctc
    20101 acaaaagacc gttccgtgcg cgagccgccc gcattcctgg cacaggggct ttccacaggt
    20161 ccggcagtac gccgttgccg gccgatcagg gtgcatctgg cagttcatat aatcctcccc
    20221 gtcttcttca ttgcccttta ctcggttgct gcgatgtttg gttcgggccg ggctccgggc
    20281 tttgctgcgc ctctgcgggc gctccttcat tcgccggttc catctccgct tcggtcaact
    20341 cccgcagacg ggagcggact tcgtagacga gacggaggct ctcatagaac ttcactgccc
    20401 gggcccatcc acggtgcacg cggtcgtcga ccgccgccca caccttcgct ggttcgaggt
    20461 cggaagcctg caactgtcga acattgatgc cggcgaatcg tccgagcatg gagaacgaca
    20521 gaatcgtcat ggccatcccc atggcgaaac gcggctgaag caccggttcc gccaaccggg
    20581 caagcatcga tcgcagactg ctgcgccggt tgccgacttt ctcacgttcg gcggaaatat
    20641 cgaacaggat ccgcgtgata agttctggcg gaggttccac cgccggcacc cgctcgacaa
    20701 aggccaacgc cgccgccgca tccttcgcca gttcggcgca ggccgcgcag cgggccaggt
    20761 gctcctccac cgcggccttc cgcgcggctt cgagggtgcc gtcgatgtag tcacagagca
    20821 ggagatccag ttccgcgcag ttcatatcgc taccctctgt ctgcgcagga cgcgcgccaa
    20881 ttccgctcgc ccacggttca accgcgactt cacagtgcct tcaggtacgc tcagcgtctg
    20941 tgcgatttcg cggtactcca tctcctccag gtcacgcagg atgaccgtct cccgcaactc
    21001 cggagagagt ttctggagag ccgcctgcaa aacctcgctg gcctcccgcc cggcgagcaa
    21061 gccgtcggtg cgggcggaaa gagcggtccg ttcctcgagc atcggaagtt gctcctcaat
    21121 cgaatccgta gcccggtcgg accgggtcct gcggtaatga tcaatcagga gattccgcgt
    21181 cagcctcgtc agccaggtgg tgaacgaacc ctcgccggac cggaaactct tcagtgttcg
    21241 gaagacccgc aggaaaactt cctgcgtcaa gtcctgcgcc tgagaagagt tgcctgtgaa
    21301 tcggtagcac agggagtaaa cgcgccgggt gtgagacttc gttagttctt cccatgcgga
    21361 ctcttctcca tcgagacagc gctctaccac actggtgtcg aggtccaagc cgtttcccgc
    21421 tactctctgg cagccatgcg cgctcgcgga ggcccagccg acaagcacgg gatcggtcgt
    21481 ccaagtcccc gggatttgtg cggcatgagc aacggctgcc ataacctacc tcgcgtagcc
    21541 catccgcgaa agccgccggc ctcgaagctt tttctgccgg ctgccttcgt ttcaggcttc
    21601 cagtagaagt aacgaggttc tatgatgata agttcgcaca aacccatggg gcggcaagcc
    21661 gtttcctatt ctatccccag catccgggga tgagaagtgc cgggaggaag tattcaaaga
    21721 aagcccaatg ttcgatggat tctaagcctc gcggctctgc tcgccgctgc catcctcctt
    21781 ttcctattcc gctggcggcg agagggtttt gattggcgcc tgtttgcttc gacactccag
    21841 agcctcgatt ggctctggct agccatcgct tgtctggtgg gcctggcaac ctactacggc
    21901 cgggtgctgc gatgggccgt tcttatacgt cccgtttgcc cgcgacccaa catgagagga
    21961 ctcttctcgg ccactgcgat cgggttcact gcgatcgtca tttttggacg cccgggtgag
    22021 tttgtacgcc cctatctcgt gtccctaaag gagaaaactc cgttttcctc gcaggcggcg
    22081 gcatggctct tggagcgaat ctttgacctg cttgccgccc tgctgatctt cggactcgcc
    22141 ttggcacaga tcggaggacc cggagtgcac acaagcccca catttgcgtg ggttatgaaa
    22201 gcgggtggtt ggatcgccgc cctccttggg ctcttgtgcc tcgttgtgct ttttctcttg
    22261 ggcagatatg ccgagtcgat gcagcagcgt ctgttgagtg ccctttccgc gctgcccgca
    22321 aggtggcaca gtaagctggt ggagttggtt gcggcgttcg tccgcggcgt cgagtcgaca
    22381 cggaatcccg gtttgctgct ctcgatactg ctatacactt ccctcgagtg gctcttgatc
    22441 gccgcctgtt acgtgagcct gttccgcgcg tttccagacc tggcatcgct ccatattgcg
    22501 gatatcatga tttttcttgg atttgtgtcg ttcggtagca tcgttcagct tcccggaata
    22561 ggtggtggca tgcaacttgt tgccatcgtg gttctgacgg agttgttcgg tgcttccctt
    22621 gaagtggcaa ccggcatggc tttgatgctc tggattatta catttgtagt aattgttccc
    22681 gtgggagtca ttatgctctt ccacgaaggt attaactggc agaaactgag agagatggag
    22741 gaccgagcgt cgatatgatc tgcccatttt gcggacaccg gcaagaccgc gtaattgact
    22801 cccgggaaag caaggaggga gacgtgatcc gccgccgtcg ccagtgtctc gaatgcgacc
    22861 gccgtttcac cacctacgag cgaatcgatg aaattccgta catggtcgtg aagaaagacg
    22921 gcaggcgtga gaagttcgac cgccagaaag tgctgtccgg gctcttaaaa gcttgcgaga
    22981 agaggcccgt gtccatggcc cggctcgccg aggttgtgga cagcgtggaa gccagactag
    23041 ccgacaaccc tgatcgtgag atatcaacta cagaaatagg agaaatgatc atcgagcgcc
    23101 tgagcgcttt ggacaagatc gcttacgttc gcttcgcttc ggtgtaccgc gatttccagg
    23161 atgttgaagc tttcctcaat gaattgaaga acttaatgcg ccaaaaacgc tcctgatttg
    23221 tggccggaag cttccctgcc ggcgccgtct ccaccgcgcg cctcggaatc cgaatgtaac
    23281 cagtaccgtt ttctaacatc taacgaaatg agaacttaac tttgcgcggg atcggtgcta
    23341 taatcgagcg gatgcccgcc aggagggcat atcctgcaag aatccgaacg tgccggagag
    23401 cccaaggcac cggaagcagt tgcgatccct tttgacttgt agtgtgttcc tgcgcgggga
    23461 ggtgagtccc aagtggatca gttcgaagag cattacctga cagacagccg cgaacccctc
    23521 aaccttcctt tcgaggaaga ggcgaagttt ttcgaccccg aggaaacgca cgacgtagac
    23581 gacttccatg ctacacagca tgtcgaggaa tcgatctaca ctgacgatcc ggtccgagtc
    23641 taccttcggg agatgggtgc ggtgccgctt ctgacccgag agggcgaggt ggatctcgcc
    23701 cgccggatgg agcgcggtaa gctccggatg caaaaggcaa tcagccgttc ggcactcgtt
    23761 cagcggatcg tcgttgaact cgccgacaac atgaagcggg cacccgagga gttggataac
    23821 tgggttgacc ttggtgatgt cgaagaaggc acgctggctg agagcaagcg gcgaagcgag
    23881 gttcttcagc ttttctcgca ggtgaccgcg ctacacaaga aacagcagca gttggccgac
    23941 aaggtcgcct ccatagccgt atcgaataag aaactggtcc gccggtggaa cgggaagttg
    24001 agtcgctgca aaatcgagac ttcccaagcc attcgcagca tcccgttcca tctctcgcgg
    24061 tggaagtatt ttgcccggga tatcgaacgg gtctccgatg agttggttca cctcgattcc
    24121 gaactgcgca gactggaagg ccgtaatggg ccaacgacac aggctcgggc gcgcgaactg
    24181 aagcgggaaa ttcggaagcg ggaggcggcc gccggcgttc cgctcgtcga tctgcgacac
    24241 acgttgcagg taatccgcca cggagaactc gaggcggagc gcgcgaagaa ggacctagtg
    24301 gaagccaact tgcggttggt ggtgtcagtg gccaagaagt acgtcaaccg cggactgcac
    24361 cttctggacc tcatccagga aggcaatatc ggcctgatgc gcgccgccga caagttcgaa
    24421 taccgccgcg gctacaagtt ctctacatac gctacgtggt ggatccgtca ggccattact
    24481 cgagctatcg ccgaccagtc ccggacgatt cgcatcccgg tgcacatgaa cgagagcatg
    24541 aacaagttcc tgcgcgccaa tcgcgagctc gagaaggagc ttggtcgcgc gcccacgaac
    24601 gacgaaatca gccgccggat ggacatcccg gtggagaagg tccaaaagct aaagactatc
    24661 tcgcgcgacc cggtgtccct cgagactccg gtcggccggg acggcgagtc cgcccttggc
    24721 gacctgatcg aagaccgctg gatcggttct ccggtcgata cggtgatcga gacgaacgta
    24781 cgcgacgaga ccgccaacat cctgaagacc ctctccccaa aggaggagaa ggtgatccgg
    24841 ctccgctttg gtatcggctg cgaacgggag cacaccctag aggagatcgg ccaggagttc
    24901 gacgtaaccc gtgagcgcat tcgtcaaatc gaagcgaagg ccttgcggca gctccgttct
    24961 cccgagaggg ctcgacacct ccgcgccctg ctcgcagcac gctaaaagtt cttcgttcct
    25021 ggaacggatt tggaccgctc gctctcgacc cttttttcgc caccgggtca ttccccatgc
    25081 agaatcctgc gaccccccgc ttagcatccc gctctaatcc agtgagggct tgggttcgcc
    25141 ctcgttggct aatcccccat acttgactgg gatttggggg attccataca atagagggag
    25201 gcgattttcg ttcactgggt gttatatgct tccggctcaa gcgattccga ggttggctcg
    25261 caccagcccg aagtctgtcc tctcacacat cggcgacacc ccgttggtta agctggaacg
    25321 gatcacgagc gaccttccgg gagtagagat ctgggcgaaa gccgagttct ttaatcccgg
    25381 agggtcggtc aaagaccgcg cggggctgaa catgatcctc gacggcgagc gaaccggccg
    25441 cctcaccccg gatcggatta tcctcgacgc gaccagcggc aacaccggca tagcctatgc
    25501 catgatcgcc gccgccaggg gctaccgagt aaaactctgt ctgccccgga acgcttccga
    25561 cgagcggaaa cggatgctcg gcgcctatgg agcggaaatc gtctacacgg accccgcaga
    25621 gggctctgac ggcgcgatcc gaaggtgccg ggagatttac gaagccgacc ccgacgctta
    25681 cttctacccc gaccaataca gcaacccagc caactggaag gcgcatttcg aaggtacggc
    25741 tgtcgaaatc atgaaccagt ccgagggccg gttgacgcac ttcgtcgcct gcatgggcac
    25801 cagcggaacc tttgtcggta caacccggcg attgaagcgg gacataccca gcgtgaagtg
    25861 ctactccgct cagccgtcct caggttttca tggtatggaa gggttgaagc acatgccgac
    25921 ggcgatcgtg ccccgcatct atgatccttc actcgcggat ggcaatctct ggctcgaaac
    25981 cgaggacgca tatgctatgt gccggaggtt ggcgcgtgaa gaagggcttc tggtggggat
    26041 ctcgtcaggt gcgaatgtgg ttgcggcgcg gacgatcggg cgccaactgg ttgaagccgg
    26101 cgagcccggg attatcgtca ccattctctg cgacggcgct gcgaaatatc tgagtgaaga
    26161 attctggaat gatccgcatt gaaagccagc cgtgggaagt gatgctttcc cacgcgcgcg
    26221 aggcgtatcc cgacgaatgc tgtggcgcca tgctcggcgc agtggacggc gacaaaaccg
    26281 ttcgaatggc cttgcctatg gagaacgtat cgcagggtcc taagcggagc cgctacgagt
    26341 tgcgtccgga ggatctcctc ggggctgaag agcgcgcccg gcaggaaggg atggatctca
    26401 tcggcatcta ccactcccat cccgactgcg acgcctattt ctccgagacg gatctgaaga
    26461 attcctgccc ctggtattcg tttgtcgtcc tctcggtgcg cggcggcggc ttcgaccacg
    26521 ccaactgctg gctgccgaac gcagaccaga ccggcgccga caaggaagaa ctgactcacc
    26581 ctgtgtaagg aacgaagaat ggccaagatt ctcataccaa ccccactccg gcagtacgtc
    26641 ggtaagctgg atgccgtcga ggtgaccggc gccaccgtcg gcgaagtgct cgcgacgctg
    26701 acgggcgagc acgaagagtt gcgccgccac ctttacagtg acgaagggaa gttgcgcagc
    26761 ttcgtcaacg tctacatcaa cgacgaggac atcaggtacc ttcagaagga caagaccccg
    26821 gtttcggacg gcgacacaat ctccatcgtc ccgagcattg ctggcggcgt cgccgtcgag
    26881 acgcccgcgc cgcccgccac cctttccaag gaagaggtgc tccggtacag ccggcacctg
    26941 atcatgccgg aagtcggcat ggacggccag ctcaggctca agagcgccag tgtgctcctg
    27001 gttggcactg gcggactcgg cagtcctctc gctctttacc ttgccgccgc cggcgtcggc
    27061 cggatcggcc tcgtcgactt cgacgtggtg gacttcacca accttcagcg ccaagtcatc
    27121 cacggaacca gtgacgtcgg ccgcaagaag ctcgactcgg ctgccgcgaa gatgaccgag
    27181 atcaacccat atgtcagcat cggccggcat gaagttgctc tctcgagcga aaacgcgctc
    27241 gacatcatca aggactacga catcgtcgtc gacggcaccg acaacttccc aacccgctac
    27301 ctcgttaacg acgcctgcgt gctgctcgga aaaccgaacg tctacggttc gatattccgg
    27361 tttgaaggcc aggcaaccgt cttcgcgtac gaggacgggc cctgctaccg ctgcctctat
    27421 ccggaacctc cacctcctgg tctggttccg agttgcgcag agggcggcgt cttgggcatc
    27481 ttgccgggac tgatcggatt ggtgcaggcg accgaaacgg tgaagctgat cctcggaaaa
    27541 ggcgagccac tggtcggccg cctgttgctc tatgacgctc tcgccatgcg ttttcgcgag
    27601 ttgaagctcc gccgaaaccc cgagtgccct gcttgcggcg accacccgac catcacgaag
    27661 ctgatcgact accatcagtt ctgcggcgtt ccccgtcagg agcaggagcc cgttccgtca
    27721 cagggccttt cggagtggca gatcgatccg gtgcaagtca aggcgaaact cgaccgtggc
    27781 gatcgcttcc tgcttctcga cgtccgcgag ccgcacgagt atcagatctg ccgcattccc
    27841 gcagcgaagc tgatccccct tggcgagctt ccgaaacgca ttcacgaatt ggattcaagc
    27901 gaagagattg tcgcatactg ccgcagcggc gtccgaagcg gcaaggcaat cgacttcctt
    27961 ggtcaggcgg gtttccgaaa agtgaagaac atggtgggag gaatcctcgc ctggagcgac
    28021 aaggtcgacc caagcgtgcc caagtattga aggaggggca cgcacccagc gtgcccctgg
    28081 cgtcttacca gtgcatgtgc agcgccagct tgatgaacct cggcaggttg cgctgcgtcg
    28141 ggtcaagctg accgaaggcc gcactcgtca cgttgttgta gcccgaggcc agactcatgt
    28201 accacgggtg gttgaacgca ttcaccatct cgaagcggaa ctgcgcccgg acgcgctcat
    28261 agatggggaa gtatttcgaa agggacaaat cccagttgtt gtacgccggt acgcggacgt
    28321 ctgggaacct cgctgggaac gtgcgcagtg tataggcttc ctgcgcggcc acacgcttac
    28381 cagtggcggg atcgttccag agtgacgtgt tgaaccattg agcgaatgtc cgctgatcgc
    28441 cgctgaggtt cgcgctgccc ggctgcacct gggctgcgtt tggataaccg accggccagc
    28501 cgaccatgta ctggatgtcc cagttcatct gccagtcgcc gagagcgtac cggatcggag
    28561 acggccagcc gccgccgaat ggccgtccct tgccgaacgg gaaatcccac acgccggcca
    28621 cggtgaaacg ctgcggtgtg tccagttcgt tcgccagcaa cttctccagc tttgtcgcct
    28681 cgggatccgc aaagacgaag tcctgcgcgt tttggaggct cacttgctcc agcgtcttgg
    28741 agatcgtgta gctcgtcagg aacgtgagcc cgtgcgccat ccgcttcgta gcctttaaca
    28801 agaaggcgtc gtaacgctgc tgtccgatcg gcacgctctg gagggttacg cctgagaact
    28861 gcggaaaggc gtacatcagg aactgccgcg agatggtcgc cccattcagc gacgcgttgt
    28921 tctcaatcag cccggccatc gggttcggga cccgaccagt gtagtatgcg gtgtcgatct
    28981 tgccgtctgc cgtgcgccgg cccagttcgt tggccggaac gtagttcagc ccgacgttca
    29041 acggcagctt acgggtaatg ttgcccgagt agccggcttc aacgagcagg ttgcccggca
    29101 gttcccgctg gatgtcgaac gagtactggt gcgagtatgg aagcacccga tcgaggtaat
    29161 ttgcggtcac gccctcgcca aggaaactgg cttcacccag ggaggaacca atcgggtcga
    29221 gcagtttccc tcccggctgg agggcgaacg gattcgtcat ctttacagcc ggcgttaagt
    29281 tgccgtcggt actgacgacg gcggtcgtcc gacggctgaa accctgcacc gagcctgctt
    29341 cgccctggcc gagatagtaa agcccgtaac cgcctcgaat cacccacttc tcgcgaatac
    29401 ggtaggaggc gccgatgcgt ggttggaagt tgttcttgtc cgcattgaac gccgtcctgg
    29461 gctggccgtt ctcgcctgca aacagcaccg cacccttcaa gttcaatccc tgtacttgcg
    29521 atgcgatcgg gctggccgcg ttgaaatcga gcccgcgaag catgcggtcg tagcgctcga
    29581 ttctcggctg ctcgtagtcc caccgcagac cgacgtttac tgtcagcttg ggcgtcacct
    29641 tccagtcgtc gttcaagaac gctgcccagt agtggttccg gtacgacggg tcgatattgc
    29701 ggtccacgta ggcgctcgtc ggatagccga gcaggaacgt cgccagttcg tcgccggagg
    29761 tcgcgtcagc ccggttggcg ttcttctgcg tccagttctt cccgaaactg taagcgccgc
    29821 tggcgagacc cggattcggg ttgttatcgt tgtaacgtcg cccttccgcg ccgaacttca
    29881 ggaaatggcg gccgacgaca aggttcaggt tcggctgaac ggtataggcg tcgttggccg
    29941 cgtagttgat cagccggtcg gtccctactg cctggtagct tccaagatca aaccgcggga
    30001 attgaagctt ggagaactgc ccgaccagtg ccgcatccat cccgagagtt gtcgggttgt
    30061 agttcacgcc gtatgagttg cccgtcgtct cctcccatcg cgccagtccg gcacggaggt
    30121 tgaaggtcat cgtcggtgag atcgtggatg tccagtccca cgtccagttg cgcccattgc
    30181 ggatcagagg cgcgttgccg gtcggctcag cggggttgtc ctcgttcaca tccttcacga
    30241 acaccagagc gcgatactcc gaaaacgggt tttggccgta ccggaaatag accgtgttct
    30301 tggagttcac cacgagatcg aggcggccga tccattggtc catgttggcc acccaccgcg
    30361 atggatacac gtagttgttg atgcgcgctg gtccgtcccc caccgcgttc ggtgctgggt
    30421 agtatttcag gacctctgta gcaaccttgc taatccggtc cgatgggatt acgttgttct
    30481 ggaacggagt tctttccccc tgcgcattgg tagttagagg atcgtagatc agcacttgcg
    30541 cgccctgcgc attaagccga gaggagaagt caccgccgcg ccactctgtt gccggaaaat
    30601 tcttgacgcc agggtcggcg gaacgctgtc gcataccttc gtacgacacc agccagaaca
    30661 gccggttccg cccgtccaat acctttggca catagacagg cccgctccct tcgatgccga
    30721 acgtgttgat gttcatcggc ggcttgcgcg tacccgtgcg attcagctcc gcttggttgg
    30781 cgttcaattc ctcggcctgg aaatactcga aggcagtacc gtggaattcg ttgccgccgc
    30841 ctttcgtgac gatcgtcacg atgccgccgc cagtgcgccc gtattgggag tcgtacgtgt
    30901 tggtcagaac cttaaactcc tggaccgttt ctatcgtagg cacatggatc acggtgcggt
    30961 tggactgaac attgctgatt ccgtccagca gaacttcgtt ttccttgttc cggccgccgt
    31021 tgatagatag ccccgaagtg ccgccgatgt cgaacgaccg cagatagcgc caacttccag
    31081 tcttgatgac gccgggcgcc gtccatgcta tctggaacgg attgcgtccc tgggtcggaa
    31141 cgtttgcgag caagtcattc gagatgacct gcgcgcgtgt cgcggactcg gtctggagca
    31201 gggagacgga ttcgctgacg gtcacgctct gcgagagttc gccaagttcg aggctcgcgt
    31261 ctatgcgtac ccgggcctgc gcttcgagca cgatattctc gcgaacgaag cgacggaacc
    31321 cctgttgctg caccgtcatt cgatacgttc ccggcgcgag gaagggcacc gtgtagttgc
    31381 ccgtcggatt tgtttgggcg ctgacggtga tgttcgtgga aagatttact acggtgatgg
    31441 tcgcgcccgc taccggagcg ccgctcgggt cgctgacgat tccggtaagg ctggcccgaa
    31501 cttcctgggc attggcagcg gagaagcaca cgaaaactaa cagaaatgtt gtgagtaccc
    31561 tggtcttcat agatgacccc tagaaggtgt tttagttcga gtgtcggtgt tcgcccgagc
    31621 agaacgagaa gtgagtgaca cggcaacgcg tgggcgtaac gacaggtcct tttcagataa
    31681 catgccgact ccccaccata gagcttgatt cgctccagaa agaattatac actggtgtgt
    31741 gcagcctgtc gacaattgta ttcattaacg attatatatt tttgtgttaa ttcttaacac
    31801 ggaataactc tatgcggaag aatgtcccag atccgatgca cagctcgcgg tcacggcgag
    31861 aggaagcgta tcgccacatt ctggcgaaga tcgcctcggg cgaactgaag gccggcagca
    31921 ccctgtcgga agtgtccttg gcccgaaacc tcggcagcag ccgcacgccg gtgcgggagg
    31981 ccctcagcca gctcgtcgcc gagggattgc tcgaacaaac cccgaaccgc ggagccgtcg
    32041 ttgtagagtt gaaacggcag gatatcgtgg agctttacga attgcgtgag gcccttgaag
    32101 tctacgctat cggcaaggct gctcgtatcc atgtgggacc cgatgagctc gatcccatgc
    32161 gccggattgt caaacaggtt ctccttctca aagagggtct ggagcgctcc gaccggaacg
    32221 tgcttgattt tgacgagatg cgccacctgg tcgcctgcga cctcggctac cattccctgc
    32281 ttgtgcgcct ggggggcaat gctcggatgc tgaaggtgct caacgaaacg cggctgctca
    32341 tccgcatctt ctcgatccat cgcagcgggc acgacgcagc gcagatcgag aacatctgcc
    32401 gccagcacat ggagattctt gacgccgtcg ccgaccggga cgccgagcgt gccatgcaca
    32461 tcctgtccgc tcacatccgg gccagccgcc aggagcgtct cgacgaatac gaccgctggc
    32521 agagggagtc ttctctccgg agcgcctttc cagctttcct cgacgtcccc agtgatctga
    32581 gccgttccgc cgactgaaga ggctccggat caacgaactg gaagtctgtg aatccccgtt
    32641 gacagtgcgt ggtggttcac cggtcctgag ccgctgccca gccctgggtt cgtgcggatg
    32701 gcttcggtga tgaactgctt ggccagttga accgcctcca caaggccgga cccgcgggcg
    32761 agagtggcgg cgattgccgc cgaatacgta caccccgtac cgtgggtgtg gcgggttgga
    32821 atctgttcgc cggggaactc gtaccacttc ccttcgaaga gcaggacatc tgtggcgctc
    32881 ccagacagat gcccgccctt taccaagaca gccttcactc cgagaccgca cagccgttcg
    32941 gctgccccgc gcatgccggt gatgtccgcc acctcgatac ccgccaaagc agccgcctcg
    33001 tgcaagttcg gagtcagtag gaaggctttc ggaagcagca gtttccggag cgcccaccac
    33061 gcttctttgg caatcagagg cgcgccgtgc ttgctgatca tcaccggatc caccaccaac
    33121 gggaaatcga agccggcggc agccccggcg accgcttcga caatctcccg gtttcccagc
    33181 gcgcccgtct tagccgctcc aggccggata tcctcaacca cagcacggat ctgctgaagc
    33241 accagttccg ccggcatgca ctcgacgcgg tccacacgca tcgtattttg aaccgtcaga
    33301 agagtgatcg ctgcttcgcc gtaaacgccg aactggtgga aagtcttcaa gtccgcctga
    33361 attccggcgc ccccgctggg gtcggaaccg gcgatcgtca acgcaaccgg tgtcttgccg
    33421 ttcatgactg gctcgcactg gcggacgcgt ggccgcgccc ggtcttcttc gcgtatccgg
    33481 cgatggcggc atggcgcagc ggccgcggca gtactttcga cagaaacaca atcacacggt
    33541 accgccatcc gggcacaacg aacaggtgac cttggtccag tccgcggagc gatgcgtcaa
    33601 ccacttcatc ggcggacatc cagaagccgt ccggaatccg cttccggtcc acgcccatca
    33661 cgtcgtggaa ttccgtgtat gtgaaccccg gacacaacgc ctggacccga accggcgacc
    33721 ccgccgcctt cagttccagg tacaacgatt cggtgaacgc attgatccag gcctttgtgg
    33781 agcaatagct gacgttgaac ggcgtctgcc agaatgccgc taccgacgag acgttgatca
    33841 cggcgccccg tcccctggta atcatgcccg gaagcgccgc gtgcgtcaac ctagcagtgg
    33901 ccaacacgtg caggcgatac atctggtcct ggctttcgac gccggcctcg tggaagcgcc
    33961 cgagtgtgcc aaaaccagcg ttgttcgtca gcaatgcgag gctctccgac cctgcgatgt
    34021 actcctcgac agactggcgt tcgctgtctt tcgtcaggtc ggctccgagc gtgtggacat
    34081 tgacgccgct cccgcctcga agttctgccg ccaattcatc gagtttgtgt ttgcggcgcg
    34141 ccaccagcgc caggtcatat ccccgcgagg ccagccgctt ggcgaagcag gcgccgagtc
    34201 cactggaagc gcccgtaatc aaagccaggg ggcgtgttgt ctgcatgtgt gtcctccgga
    34261 tttgctcttg ttcccaaact ccaatagccc ttcttgtcta tgttactttg aaggtctccc
    34321 gcatctcccg cgggctgtag gaacgcagga accatgcgcc tgggaatcga cttcggcacg
    34381 acgcgcattg cggtggccgc cgcggaccgt ggcaactacc cagtggtctc cttcgagtgc
    34441 cccgacggcg agacgcgcga ctggtttccg ccgctggttg cggtccgagg gtccgagatc
    34501 tgctacggtt gggcggccca tatgtcccag accggcgacg gatggaccgt cgtccgctcc
    34561 ctgaagcgcc tgctcggaag aaccggtctc aatacacccg tgcagattgg cgaccggctg
    34621 atccacatga accggttgct cgggggtctc acttccaccc tgcgtgacga gcttcgcgaa
    34681 cgctccaatc tccgactcga ccgcggcgaa ccgctggagg tgatgctcgg tgtcccggcg
    34741 aattccaaca gcaatcagcg ttttctcacc gccgaggcgt tccgcctggc cggcttctcc
    34801 gtcatcggag ttctgaacga accgtccgcg gccagcatcg aattcggcca caagcagcgg
    34861 ctcagcgacc aggtgcgcga gcgtggttgc gtgctcgtgt acgacctggg cggcggcacg
    34921 ttcgacgctt cgctggtcga gatggatgag cagacacact ctgtcctcgc ttcggacggg
    34981 atcgagacgc tgggcggcga cgattttgat gagatcctcg ccgaactagt gttcgagagg
    35041 gccgggttga gcgaggccga tcgcgatagt ctgcggcagg atgaactgtt tcgcctgcac
    35101 gaagaatgcc ggctgaagaa ggagtcgatc cacccaaact cgcggcggat cgccgtagat
    35161 ctcgacagcg tccgcgcggg ctggggcgag gtcgccgtgc ccgccgccga gttttatgaa
    35221 gcgtgcaggc cgctggtcca ggagacgatg aacaccgtgg agaacctgct cgcgtcgcac
    35281 gggctcggca ccaatgatcc acggcctgca gtcgaacaat accgcctcga ggcggtctac
    35341 gtcaccggcg gcggtagcga gttaccactg gtggcacgcg aacttcggga gcgctttgat
    35401 cgccgcgtac gccgctcgca gcatgcccgt tccgccaccg cgatcggcct ggcgattcag
    35461 gcagacgtgc agtccggcta cacgctacgc gaccgattca cgcgctactt cggtgtttgg
    35521 agggaggccc aaggcggctc gacggtgtgg ttcgacccgc tctttcagaa aggtgaactg
    35581 cttcctggtc ccggcgaatg ggcgctcgaa gtcaaccggc gttactcgcc tgttcacaac
    35641 atcggccact tccgttacct cgagtgcagc cgcataggag aatccggcga gcctgcgggc
    35701 gacatcactc tctgggatga gatccgcttc cccttcgacc cggttctgga gcagcgtcgg
    35761 gatctggact cggttccggt ggaacacacc gaggccgccc gcaaccagtc gatcgaggaa
    35821 gtctacacat gcgatgcagg tggcgcgctc ggtgtgacaa tcgccaacct gagcgccgac
    35881 tacaaacgta cctaccgcct tgcccggtgg gccgcccggg aagctccggt caaacccgcc
    35941 cgccgccggc cggccacgcg aagaatcgtc tcccgtagct gacggctcat ggcagggtgt
    36001 ttccctctac ttacctccgg cgcctccacc ggacctctgc ttcacgacgt atcgcccgat
    36061 ggattcgacg gtgtccagcc agatgccgtt ggccgggtct ttggcgtagc ggcagaattg
    36121 ttcgagtgcc gcggcatcgg tcgtctgaaa ggcggtcttg ccgatttcgt gcccggcaaa
    36181 gatcacccag cggccctctt tgagtgcggt cgaggcgtgt ttcaccatcc cgtcgaaggt
    36241 cagctcgtcg atgcccgtcc ccatcaactg cgccatgtcg cagatctcag ggtcgttcgc
    36301 cgcctcgtcg cgatacccgc gcccggagag gaaccctcgc gccaccagcg gcacgtagct
    36361 tttcgtctcc aggccgcggc ccacgaattt cagcccgcag ggatagccaa agctgacggg
    36421 cttgacgcca aggaggcttt gaatgatcgt attcgcgccg tccagttcct cttccatgcg
    36481 ttcgagcgtg tagccttcga gcgcgttcct cttcgagaag ccgtagttgc ccgtgcaggg
    36541 atgggatcgg gaatgattcg cgatctcatg ccccgcagcc actgctttct tccagccctc
    36601 aatccgtttc tccaggttgc cgggtgaaac gtagaacgtc gccttcacgc cgattctgtc
    36661 gaacagcggc ataccaacat cgatctggct caggcgggcg tcgtcgaacg aaagactgac
    36721 agccgcccgt tttccgtccg gccagcgaaa cgcggacttc tgctgcgcgg gagcggccat
    36781 ggccagcaga agcaccagaa atgggatgac ccgtggaagc atgagactac tccttcacgg
    36841 tgatcttgcc gaggttgtac cagccgtcct caccgatgcc ggcgatggcc agcttcacct
    36901 tcgcctggcc gctgaccgga tcgatgagag cgatgccgag atcgtattct cccaccggca
    36961 tctccgcggg aaggaagaag gcgctgtcat agagattgtc gccgggcagc cagtttcgga
    37021 tatccgcctc ggtgcgccac accatggtgt gctgagcgtt cttcatccgg atcgccagcg
    37081 gccactgcgt gtaggacgga gccactcctt tgttttccca ccacgaggtg aacggcacct
    37141 tggcattctg ggacacgacg ccggggtaag tgaacttccg cagaacgaaa cggtagccca
    37201 tcttcttgag ccaacggtcc acgttcggct tccacttctc cggaaccgcg gaagacttcc
    37261 cgttgaacgt actgatgtgc cacttcagcg actgatcgat gatgtagtcg acgtcccatc
    37321 ctttgttcag ccagtgctgc atcacccagc aaacctcgaa gctgaccggg gccttcttcc
    37381 aggcgtcctg catgccgaaa ttgatgatcc cttccgggta gtagtcgttc atgtgggacc
    37441 agttcggatt gttaaaaccg cccatgtcac cgaggcaatc cacacgccag ccgacgtccc
    37501 gcttcgagat gccgtacttg ttcgtctttt cgtcggtgag cagcatgacc agcggggttt
    37561 tctggaaccc ttcgaggtag gcatcaacga gggcctcgcg ggtcggctgc gtgagttcgg
    37621 cggaacccgc tccctcgccc cacgcgccga cgatcgacag atcgacggcc tcgaggtcag
    37681 ggtggccgtc atagcgcgcg gcgacggccc ggaccatggc gccgaaatgc ttggcatagc
    37741 gcggatcttc cggatcggtg cgccacttct tgatcattcc ccggctcgct tcgtcgccca
    37801 tcatcttgcg gtaccagtcg ggcacgtcgt tgtcctcggt agtgccgtac ggcgcgatgc
    37861 ggagccagag cgtctgtttg cgcgaatgcg ccgtcttcag ggccgtgtcg agcatctgcc
    37921 agttgaactt tccctgctcc ggctcgacga acttccagta gatgcggaag taggcgatcg
    37981 tggtcgccgg atgattcttg ttttcgaggg aacccttgta ctcctggtag gcgatcgggt
    38041 aaccctcggt ccatttgagg ccctggttca gctcgtcacc attgaagcgc tggaaggttg
    38101 tgaacccgat gccggggttc agtagcacat cgtcgatctc cgtgggacgg acgatgacag
    38161 tctgctgcgc gagagcgggg gcagcgatca gtactacaag aaaggggaac agatatttgt
    38221 gcatgttcat tcctcaatac atccgtgagc gtatccacgc ttccatgaaa tccttgccat
    38281 gataacggat cgtccggtcg tcgacgacct cggcgccggg cctgacattc gcgtcgatcg
    38341 gtagggaatt ccgcgttgta tattcgatct cgatcgtgac cggcccgtcg atcttgtacg
    38401 gtttgacctt gccgattttc tgcatggaca gccgggcctt ctcgcggatc agatcgcggg
    38461 cggccggtgc ggacatcgag atgcaactgt aacgggcgag gccttctttc acttccgcaa
    38521 cctcagcgtc gggcacgatg gcgcgcagtt cctcgatggc ggcgcggtct cccgtgatca
    38581 ggatcgtcgg cacgccgaac cagcctgcca gcgcgacgcg ggtctcgact tcgcccaccg
    38641 gcttgccgtt catccgcatg ttctggatgc caagcgagct gtagctgtgc gccatgatgc
    38701 ccgctcgcac atttgccatg ctgtgctgac cgaggaagac cagggcggag taacgccgct
    38761 cgagcagtcc ggtcgggccg aggctgccga taatgagctt cgccttcggg tgaatcgtga
    38821 gcgcggagag cgtccggctt ccgtcatgag cgtcccacac gatgacttca tcggcgccgc
    38881 ccgcaagaaa accttcgacc gcggcgttga tctcgcccgt gagcaactgt cgcaactccg
    38941 tatcggtcgg atcggtctgc tcctggcggc agatgccggc gacgccctcg gcgtcggtga
    39001 ccatgaagac ggtcttcttt gcctgggccc acgaaacggc ggccgaggcg agcacgatca
    39061 gaaccagatg cgcctgtctc atacggacag tatagttgcg atcatccgga cgcgaggctg
    39121 cgctatcatg gcaccttcga aggcagtcat gttctcgacg aagcgatctc agctactggt
    39181 ggcgctcacg atgtccgtca tagatccaat tggctctcga cttcaggccg ccgacgatgc
    39241 gattcgaagc gcggcgacgt tctatgcttc cttcgattcg gaggtcaaag gcgacttcgg
    39301 cggcggttcg ttgaagccgt ctacccgctt caaccacccg accgcgaagg ggcaattcgt
    39361 gttcgagccg ggattcaacg cgaacgtctt tcggatcgcc cagaacaagg gtatccgcgg
    39421 cggagcgcta gagccgatgg acatcctccc gcgaaacgga cggatattct ttccagctcg
    39481 cggcaacatc gcgttccgcg aggccgggtg gtccggaagt gtgtcatttt ggctcaaaac
    39541 cgacccaaat acgatgctaa agacgtcgtt ttgcgaccct ctgcagatca cgcagaaggg
    39601 cgcgagcaac ggcggtctct ggctcgactt caatgatgcc aagcccaagg acatgcggat
    39661 ggggtccttt cccgccgttc ccgagggcga aaaagcggtt tcggagaacg acccgcaggc
    39721 gccgctcatt cggatgtcca ccgagggatt cacggaggac cagtggcgcc atgtcgtcct
    39781 tgtctgggag aacttcgaca ccggccgtgc cgacgcacgc actgcgctct acgtggacgg
    39841 caaactgatc ggagagttga aagaccgcga gatcgccatg ggttgggatc tcgacaagac
    39901 gggcatctac gtggccgtca gctatattgg gctgctcgac gaactggcgc tcttcgaccg
    39961 gccgcttacg ccggcggaga tcacccggct ccgggaggaa cccgggttgc tggccgcgct
    40021 gaagtaacgc cgctagcgtt tcgcgattgc ctcgccggcc tcgatccggg ctttctccca
    40081 ctcgtcgggg ttgttcttcc agatttcggt gtcgaggagg gctgcctcgc cgaacggccg
    40141 cttatagaca atccggttta cgatggcgtc ggcggcgtcc ttgccgccgg ttgtctccga
    40201 aagtagccag aaatactcgt ggtcctgaag cccgcggcgt tgagccttga gccggatgct
    40261 cggaatcggt tgctggtacc ccatgacctg cccgcggtag atcaactgac ccgagccgtt
    40321 gtaagtacgc tgcggttcct tgaagttttc cgcttcgtac caggcggcgg aggcgttcca
    40381 atcgaactcc cactccaccc aaccggagcg gtacttccag ccgatccagc cgatggcgcg
    40441 gttgacgagc aggtcgagat cggtgaacgt gttggagccg cagccggaat tcttgcgttg
    40501 ctcatagatc atcgggccgt aaaaccagct ctcgacgccg cgcttgcgga aatgctccat
    40561 cttttccgca tcccacgaga cggtgtggca aacccacagg gtgaccactt cctgaagttt
    40621 ctccatcgcc tcccaggaat agccgccgtc gattcggtac ttgaaccagt cgcggcccat
    40681 cgcgctttgg agcagccgcc cgtacgagat catcttctcg taggcttcgt cgttgtaaga
    40741 ctcg
//
LOCUS       DATIFQ010000416         6254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_524232, whole genome shotgun sequence.
ACCESSION   DATIFQ010000416 DATIFQ010000000
VERSION     DATIFQ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524232"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VLE22_21010"
     CDS             complement(<1..397)
                     /locus_tag="VLE22_21010"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSB16945.1"
                     /translation="MNDWSPSSWKSKPADQQPSYPCQDDVDRVVEEISKLPPLVTSWE
                     VGLLKSHLGQAARGERFLLQAGDCAERFDLCTSGPIANKLKILLQMSLILIHGSDRPV
                     IRVGRFAGQYAKPRTDYTETRDGRTLPSYR"
     gene            731..4051
                     /locus_tag="VLE22_21015"
     CDS             731..4051
                     /locus_tag="VLE22_21015"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB16946.1"
                     /translation="MSQTKVLIFVVIMLFAFSATAHSQTTFATITGTVTDATGAVVPG
                     ATVTATNVATNIKATTQSNEAGNYTIAQLKEGTYSVRGEAAGFMSFVVDSVALVSRDV
                     RRVDIRLEVGAVETAVEVKGGATLIETETARITSTKTTLELNTLPTNSRGLWAYLNLA
                     PGLQGQDGSSVTRFAGSRVNENNWSIDGTTFSDGVDNTQTGPLANYIESFQEVKIDLS
                     NNSAEFGSVGQVTIISKSGTNDLHGALFDYYSTPWFRAKCFFCSSRGTGIRHAPGGAA
                     GGPVWLPKIYDGRNRTFFYFSYETARGSAVSQVLNPMVAPSAWRNGDFSNLSTPIIDP
                     TTGQPFEGNKIPEDRINSVSKKIQDKFFPLPNYGDLVNLHSQNYRELRVRAYDPSTYW
                     TTRIDHKVRDGDQIFGRYTWSRLYNRPWEGNLPTIGLRWQQRDDRAATTSWTHSFRPT
                     LLNEFRWGFGLNNNPINYDFGKGSTQHGVDLVKELGLVGLAPDLPDINGILNITFNNG
                     MTGLYQYPWRKKGYRTHTEEIQDHVSWFKGRHNLKFGFYLLRAEYDDFGASGNLFGNV
                     RFTDRFTGQPYADFLLGLPTTASRAFPPVEVARNRWSYDFFATDDFKLSPKLTVNVGV
                     RYELHLNWRENNGLMSLFDIKSGKLIVPDGALSKVSAIFPKDYVGVAEASSVGWEPQT
                     LVRQDKNNIAPRIGLAYRPWGNDTVFRAGFGMFYNVVPFVYVLNFGDVPFLLAEPSYN
                     NPKDNPQVIFPRVFPSTGTGGPSEVSLSAAENPDYRTPYSLQYNFTIERQQWNTGFRL
                     SYIGTAMRKGPWQYNYNAPVANDQPFISKGRAFQNYPEIWYVTNGAGHQYNGLTAEAL
                     RHLAGGLYFQGSWTWARDRYDLDYNWDFGGEKFISEDPRNRKREIGPAQEIPTHRFST
                     NFIYQLPFGKGRHFGSNVSRLGDLLIGGWELSGIYTAQTGQFLTPFWSGDDPVGISFT
                     DSDTPASVTLRPDVLRNPNFSSGQRTIEKWFDTGAFAPPQAGSFGTSGKGVIIGPGVN
                     VWSVGFAKEFGLYERARLRWEMTATNFFNHPNWVNPSTDITDSSVGEITSDGGVTSGS
                     VGDRAGSRNFRMGLRLQF"
     gene            complement(4131..5411)
                     /locus_tag="VLE22_21020"
     CDS             complement(4131..5411)
                     /locus_tag="VLE22_21020"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB16947.1"
                     /translation="MELTRRQIMKAGLAAPATFARQYSQPVAALSSRKIRITGVETFA
                     VRNPPPSKGGSAWVFLKLLTDSGVVGYGEVDLLGISFRPPAVEILINDIVGAAVAGAN
                     PYQVERLYDKLYNLNYSHYPEFTKMGVISAIEMACWDIIGKDTGRPVYDLLGGAVRER
                     IRTYTYLYAGKSDKGRRTTWLDPAYCAERAAAYVAQGFNAVKLDPIQQHRQVYEDYQQ
                     VMPVQESLEALVTAEASIKAIREAVGNKCDVLIGTHGQMTAAGAIRLAKRLEPFDPMW
                     FEEPVPPENMTEMAKVARATSIPICTGERLTTKYDFARLIECQAAAIFNYDVGHVGGI
                     LEAKKIAGMAEAYYLHITPHVWDGPIVAAASIQIAACCPNFLIIESIEDMGGIHAELL
                     KEPIEWKSGYIIPSTRPGIGYELNEAMARKYKWS"
     gene            5607..6248
                     /locus_tag="VLE22_21025"
     CDS             5607..6248
                     /locus_tag="VLE22_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16948.1"
                     /translation="MRKSLVIAVASALFLVGASCSIRNNKTTTANVPSGPVRAQVTEP
                     GVIPAGTTLVIRTNDEIKTQQAGKTYSAQVAETVENQSGQVLVPKGSPAELIVLQTSS
                     GGTTGTASLALGVRSITVNGKTYSIATDTHEQRGGEGLGANRRTAEMVGGGAVLGTLL
                     GAVAGGGKGAAIGAVVGAAGGAAAQVLTRGDEVKVPAETLLTFRLDQPWRMTS"
BASE COUNT         1255 a         1919 c         1824 g         1256 t
ORIGIN      
        1 cccggtagct cggcagcgtg cggccatcgc gcgtttccgt gtagtcggtc cgtggtttgg
       61 cgtactgacc cgcgaagcgg ccgacgcgga tcaccggccg gtcgctccca tggatcagga
      121 tcaggctcat ctggagcaga atctttaact tgtttgcgat cgggcccgac gtacacaaat
      181 cgaatcgttc ggcgcaatcg ccggcctgaa gcagaaacct ctcgccccgc gctgcctgcc
      241 ccaggtggga cttgaggagg ccgacttccc acgaggttac cagcggcggg agcttcgaga
      301 tctcttcgac cacacggtct acgtcgtctt ggcaagggta ggacggctgc tggtcggcgg
      361 gtttcgactt ccaggatgaa ggagaccaat cgttcatggc tttcccctat agcttcgcac
      421 cgcgcctgca accgcgggtg ctcctcaatc agaaaccgtg cgtctggcaa caccggaatc
      481 cttacgaccg gaagtacgca ccccgaagcg gcaagggagt cccttgtaag ttgctgattt
      541 gtaaacgtta atgaaggcat caaaaacctt gactctggat gccgtcgctc ttataattgg
      601 gccgtaattc ggcttcaggg tatcgggtga tctttatccg gttcgttgcg gttgccgttt
      661 cgtcggcggc caagacagcg ttgtgtagac agttctgacg ttccggagcg gcatcatagg
      721 aggctatgcg atgtctcaaa cgaaagtgct gatcttcgtg gtgatcatgc tgttcgcgtt
      781 ttctgcaaca gcacactcgc agaccacgtt tgccactatc accggcacag taacggacgc
      841 cacgggcgcg gtcgtgccag gcgccactgt gacggcaacg aacgtggcta ccaatatcaa
      901 agccacaacc caatcaaacg aagccggcaa ctacaccatc gcccagctca aggaagggac
      961 gtattctgtg cgaggtgagg ctgcgggctt catgagcttt gtcgttgaca gcgttgccct
     1021 tgtgtcccgc gatgtgcgcc gcgtcgacat tcgtcttgag gtcggcgccg tggaaacggc
     1081 cgtcgaggtc aaaggcgggg caacgctcat cgagacggaa acggctcgca tcaccagcac
     1141 aaaaaccacg ctggagctca acacgcttcc cacgaattcg cgggggctgt gggcgtacct
     1201 caacctcgcg cccggccttc agggacagga cggcagcagc gtgacacgat tcgccggcag
     1261 ccgagtgaac gagaacaact ggtccatcga cgggaccacg ttctcggacg gcgtggacaa
     1321 cacgcagacc ggcccgctgg ccaactacat cgaatccttc caggaagtga agatcgatct
     1381 gtcgaacaac tcagcggaat tcggctccgt cggccaagtc acgatcatct ccaagtcggg
     1441 cacgaacgac cttcacggtg cactgttcga ctactactcc acgccatggt tccgtgctaa
     1501 gtgcttcttt tgtagctccc gcggtaccgg aatccgccac gcgcctggag gcgccgccgg
     1561 tggtccggtc tggcttccca agatctacga tggacggaac aggaccttct tttacttttc
     1621 gtatgaaacg gcgcgcggaa gcgcggtttc acaggttctg aaccccatgg ttgcgccgag
     1681 cgcctggaga aacggagact tctcgaacct gtcgactccg atcatcgacc cgaccacggg
     1741 acagccgttc gaggggaaca agattcccga ggatcgaatc aattcagtct cgaagaagat
     1801 ccaggataag ttctttccgc tgccgaacta cggcgatctg gtgaatctcc acagccagaa
     1861 ctaccgggaa ctgagggtgc gggcttacga tccctccacc tactggacca ctcgcattga
     1921 ccacaaggtc cgtgatggcg atcaaatctt tggaagatac acttggtccc gtctgtacaa
     1981 ccgtccctgg gaaggcaacc ttccgaccat cggcctgcgc tggcagcagc gtgacgatcg
     2041 cgccgcgacc acctcgtgga cccattcgtt ccgtccgacc ctgctcaacg aattccggtg
     2101 ggggttcggc ttgaacaaca atcctatcaa ctacgacttt ggcaaaggtt ccacacagca
     2161 cggcgtagac ctggtcaagg aattgggatt agtcggcctt gctcctgatc tgccggacat
     2221 caacggaatt ctcaacatca cgttcaacaa cggcatgacc gggctgtatc agtacccgtg
     2281 gcggaagaag ggctaccgca cgcacaccga ggagattcag gaccatgtga gctggtttaa
     2341 gggtcgtcac aatctgaagt tcggtttcta cctgttgcgc gccgaatacg acgacttcgg
     2401 cgccagtggg aacctcttcg gaaacgtgcg gttcaccgat cgcttcacgg gccagccata
     2461 tgcagacttt ctgctcggct tgccgacgac ggcatcccgc gcattcccgc cagtggaggt
     2521 cgccaggaat cgctggtcct acgacttctt cgccactgac gatttcaagc tcagcccgaa
     2581 gctcaccgtt aacgtgggcg tgcggtatga gctgcacctg aattggcggg agaacaacgg
     2641 cctgatgtcg ctgttcgaca ttaagtccgg caagctcatc gtcccggacg gcgctctttc
     2701 caaggtgagc gcaatcttcc cgaaggacta cgtcggcgtc gcggaagcca gttcagtcgg
     2761 ctgggagccg cagacgctgg tccgccagga caagaacaac atcgcgcccc gcatcggact
     2821 cgcgtaccgt ccgtggggca acgacacggt cttccgagcc ggcttcggaa tgttctacaa
     2881 cgtcgtgccg ttcgtctacg tgctgaactt cggcgacgta cccttccttc tggcggagcc
     2941 gtcttacaac aacccgaagg acaaccctca ggtgatcttc ccgcgggtgt tcccgtcaac
     3001 cggcaccgga ggcccgtccg aggttagcct ctctgccgcc gagaaccccg actacaggac
     3061 tccatactcg ctccagtaca acttcaccat cgagcggcag cagtggaaca ccgggttccg
     3121 tctctcttac attgggacgg caatgcgaaa gggaccgtgg cagtataact acaatgcccc
     3181 ggtggccaac gaccagccgt tcatctccaa gggaagggcg ttccagaact atcccgagat
     3241 ctggtatgtc actaatggtg ccggccacca gtacaatggg ctcaccgcgg aggcgttgcg
     3301 ccacctggcc ggcgggcttt acttccaggg ttcgtggacc tgggcgcggg atcgctacga
     3361 tctggattac aactgggatt tcggcggcga aaagttcatc tccgaggacc cgaggaaccg
     3421 caagcgcgag atcggccctg ctcaggagat cccgacccat cgcttcagca ccaacttcat
     3481 ctaccaactc cccttcggca agggccgcca tttcggttcc aacgtttcga ggctcggcga
     3541 cctgctcatt ggtggttggg agcttagcgg catctacacc gcgcaaaccg gccagttcct
     3601 gacgccgttt tggtcaggag acgacccagt cggcatctcc ttcaccgact cggacacgcc
     3661 ggcatcggtc accctccgcc ccgacgttct gcggaatccc aatttttcca gcgggcagcg
     3721 gacgatcgaa aagtggtttg acacaggcgc attcgctccg ccccaggccg gctcctttgg
     3781 cacatccggc aagggcgtga tcataggtcc cggcgtgaac gtctggagtg tcggattcgc
     3841 aaaggaattc ggtctctacg agagagcaag actgcgctgg gagatgaccg ccaccaactt
     3901 cttcaaccac ccgaactggg taaatcccag cacggacatc accgactcct ctgtgggcga
     3961 aatcaccagc gacggtggcg tgacgagcgg atcggttggg gaccgggcag gctcacgcaa
     4021 cttccgcatg ggccttcggc tccagttcta gccgtttgtt gtgatgggga gcctgccgct
     4081 gggcgggctc ccctactctc gcctggcgac ctcttcattc attgtcgcaa tcagctccat
     4141 ttgtactttc gcgccatggc ctcgttcagc tcatagccga tgccgggtcg tgtcgaaggg
     4201 atgatgtagc cgctcttcca ctcgatcggc tccttcagca attccgcgtg gatgccgccc
     4261 atgtcctcga tgctctcgat gatcagaaag ttcgggcagc aggccgcgat ttgaatcgac
     4321 gccgcggcga cgatcggtcc gtcccagacg tgcggcgtga tatgcaggta gtacgcttca
     4381 gccatgccag cgatcttctt cgcttccaga attccgccga cgtgcccgac gtcatagttg
     4441 aaaatcgccg ccgcctggca ttcgatcagg cgagcgaaat cgtacttcgt cgtcagtcgc
     4501 tcgcccgtgc agatcgggat gctggttgca cgcgccacct ttgccatctc ggtcatgttc
     4561 tccggcggca ccggctcctc gaaccacatc gggtcgaacg gctccaggcg tttcgccagc
     4621 cggatcgccc ccgccgccgt catctgccca tgcgtgccga tgagaacgtc acacttgttg
     4681 ccgacggctt cgcgaatagc cttgatggaa gcctcggcgg tcaccagcgc ctcgagtgat
     4741 tcctgcaccg gcatcacctg ctggtagtct tcgtagacct gcctgtgctg ctgtatcgga
     4801 tccagtttca ccgcgttgaa tccctgcgca acgtacgctg ccgctcgctc ggcgcagtac
     4861 gcgggatcaa gccatgtcgt ccgcctgcct ttgtcggact tccccgcgta gaggtaggtg
     4921 taagtccgga tgcgctcccg caccgccccc ccgaggaggt cgtagacagg ccggccagtg
     4981 tcctttccga tgatgtccca gcacgccatc tcgatggcgc tgatgacgcc catcttcgtg
     5041 aactcggggt agtgactgta gttcaggttg tagagtttgt cgtacaaccg ttccacctga
     5101 taggggttcg cgccggccac cgccgccccg acaatgtcgt tgatgaggat ctcgaccgcc
     5161 ggcggccgga aagagattcc tagcaagtct acttcgccgt atcccacaac tccggaatcg
     5221 gtaagcagct taaggaaaac ccacgcggaa ccgcctttcg atggtggcgg gttccttacg
     5281 gcgaatgtct cgacgccagt gattcgaatt ttgcgagagc tgagcgcggc gactggttga
     5341 gaatactgcc tcgcaaacgt cgccggagcg gccagccccg ccttcatgat ttgcctgcgt
     5401 gtgagttcca tgatgagttc tccctattga agcaccgacc gcagccactg tgtaaagatt
     5461 accggtcgcg ttttcctaag tcgctgattt caggtacgcg aaggctggca cggtgtttgc
     5521 agaactcgct ggtgactggg acgcctcctg tacatgacgg ataccaatgt tccgtcacaa
     5581 gggagacgag aaaggagatg gggcaaatga gaaagtccct cgtcatcgca gttgcgtcgg
     5641 cgcttttcct cgtcggtgca agttgtagta tccgaaataa caagacgacc acagcgaacg
     5701 tgccgtccgg accggtacgc gcacaagtta ccgagccggg cgtgatcccc gccggcacca
     5761 cgctggtgat ccgaaccaac gacgagatca agactcagca agcgggcaag acgtactccg
     5821 cccaagtggc ggagacggtg gagaatcagt ccgggcaggt tttggtgcct aaaggctcgc
     5881 ccgccgagtt gattgtgttg cagacatcca gtggcggtac aacgggtacg gcgtcactgg
     5941 ctctgggtgt ccgctcgatt actgtaaacg gcaaaaccta cagcatcgct accgacacgc
     6001 atgaacaacg gggcggggaa ggcctggggg caaaccgcag aaccgcggag atggtcggag
     6061 gcggcgctgt tcttggcacg ctccttggcg cggtcgcggg gggcggaaaa ggtgcggcga
     6121 tcggcgccgt cgtgggagcg gccggaggcg cggctgcaca ggtgctcacg agaggcgatg
     6181 aagtgaaagt gccggctgaa acgctgctga ccttccgcct tgatcagccc tggcggatga
     6241 ccagttgaaa gacg
//
LOCUS       DATIFQ010000417         3351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2057736, whole genome shotgun sequence.
ACCESSION   DATIFQ010000417 DATIFQ010000000
VERSION     DATIFQ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057736"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..754
                     /locus_tag="VLE22_21030"
     CDS             296..754
                     /locus_tag="VLE22_21030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16949.1"
                     /translation="MLRASTYLATLVLALTGPVTPALVWAEEPTSIRQILEEPQVYHL
                     RHVTLLGTVRDVQPLDPYTLPNGTNCYGAYLFRLEDDTSTLPVAVLGICGKPLVRDPE
                     VEDGARIEVSATIQAPSHGGYYLSFRGLQVVTEQEGVVQAVADRILPIVE"
     gene            887..1450
                     /locus_tag="VLE22_21035"
     CDS             887..1450
                     /locus_tag="VLE22_21035"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSB16950.1"
                     /translation="MAWILLAVLGVLVLLVIGMYNSLVRLKVQSENAWADIDVQLKRR
                     YDLIPNLIETVKGYAGHEKQTLEAVIAARNRAMTATTPSTKAEAEGVLAQSLKSLFAL
                     AEAYPQLRAVESFTQLQSSLNQIEEAVQNARRYYNAVVRDLNTKIQEFPSNFIANFFS
                     FKPREFFEIAEAAERAVPKVGFEQASR"
     gene            1487..3232
                     /locus_tag="VLE22_21040"
     CDS             1487..3232
                     /locus_tag="VLE22_21040"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HSB16951.1"
                     /translation="MRAPNVIRLGLLLLGWLLTVGSTPAHARSLVIEQFQADIQVLSS
                     GELLVTETIRPRFTGSWNGLKRDIPVEYRTPQGFNYTLLLELVSVTDEQLAPLRFESY
                     RDRHYRSFKIWLPGAQDTTKTLILTYKVSNGLKYFEDHDELYWNVTGDEWDVPIESAA
                     ARILLPPGATGVKALAFSGAYGAREQQADVRITGPEVLYRMTRPLGFREGLTAVVGWD
                     KGFVAEPGPLQLTKLFLRSNWPLALPIGVFGLMWYLWYTRGRDPRLRPITVAYEPPDT
                     LTPAELGTLIDNSPDLRDITATLVDLAVRGFLRIEERQESKLLGFWSSSAFYLHRLKT
                     ASEWTDLKPHERAIMNGIFSDETTTVVALSDLENRFYAHLDGIKSSLFEQLLSRGYYA
                     RRPDRVKQVYLTVGIIVAIASIFGAFWLSEHYGIALQTGLAAGILSGLIIVGFGWFMP
                     ARTMRGARALEKVLGFEEFLTRVESDRFQRVVKTPQMFEKFLPFAMALGVEQNWARAF
                     DGIYTQPPAWYQGADIVDFRPRSFTGNLSQMSAAAGAVMTSAPRSTGASGFSSSGSSG
                     GFSGGGFGGGGGSGF"
BASE COUNT          652 a         1076 c          975 g          648 t
ORIGIN      
        1 tcggtcatat actggacggc aagatccgcc atgcgccgca tatcgcccgc gcgggaggga
       61 tccatgctga tcgacggcac ctccaccatc tgccccaaca tgtcctcaaa ccgcgaccgc
      121 actccgttca cataggtctg aagttgctgc tcgtgcacca tgctcgccgt cctccctggc
      181 caatcaatgg aggcggatta taacgaccgg gcacgggaag agaaagacac gcgaacgatc
      241 ttctagggcc aagaatgcta gaatagccgc attcgccatg gaggatggtg cccgtatgct
      301 ccgcgcatcg acatatcttg caaccctcgt actggcactc acgggcccgg tcacgcccgc
      361 actcgtatgg gctgaggaac cgaccagcat ccgacagatc cttgaggaac cgcaggtcta
      421 tcatctgcgg catgtgacgc tgctcggcac cgtgcgcgat gtgcagcccc tcgatcccta
      481 cacgctgccg aacggaacca actgctacgg cgcctatctt tttcgcctgg aagacgacac
      541 ctcgacgctc cccgtcgcgg tgctcggcat ttgcgggaaa cccctggtgc gagatcccga
      601 agtggaggat ggagcgcgaa tcgaggtgag cgccacaatt caagcgccca gtcacggcgg
      661 gtactacctc agcttccgcg gattgcaggt ggtcaccgag caggaaggcg tcgtccaagc
      721 cgtcgccgat cgcattctcc cgattgtcga gtaacggcgc acctccggcc gcctcacgtc
      781 gactcacagc gcattgcttt acagttcgca ccctcggggg tatagtccca gaaaagtttt
      841 ctgcgcgacg gcggccccgg tttccggtgc atagagagga ggtcccatgg cctggatact
      901 attagccgtg ctgggcgtac tggtgttact ggtcatcggc atgtacaaca gcctggtccg
      961 gctgaaggtc cagtcagaaa acgcctgggc cgacatcgac gtgcaactga agcgccggta
     1021 cgacctcatt cccaatctga tcgaaacggt caagggctat gcgggccacg agaagcaaac
     1081 gctcgaggcc gtgatcgccg cccgcaatcg cgcgatgacg gcgacgaccc cttccaccaa
     1141 ggcggaagcc gaaggcgtac tcgcccagtc gttgaaatcg ttgtttgctc tggccgaagc
     1201 ctatcctcaa ttgcgcgcgg tggaaagctt cacccaactc cagagctcac tgaaccagat
     1261 cgaagaggcg gttcagaatg cgcgtcgcta ttacaacgcc gtggtccgtg acctgaacac
     1321 caagattcag gagtttccat ccaactttat cgccaacttc ttcagcttca agccgcgcga
     1381 attcttcgag atcgccgaag cagcggaacg ggccgtgccg aaagtcggct tcgagcaggc
     1441 gtcccgctga tgtcagccgg cagaccggtg atggcacccc ttccctatgc gcgcacctaa
     1501 cgttatccgt cttggcctcc tgctccttgg ctggctgctg acggtcggga gtacgcccgc
     1561 tcatgcccgc tcgctcgtga tcgagcagtt tcaagccgac attcaggtcc tctcaagcgg
     1621 cgaactcctg gtcaccgaaa cgattcgccc gcgcttcacc ggatcgtgga acgggctgaa
     1681 gcgcgacatc ccggtcgaat atcgaacgcc acagggcttc aattacacct tattgctgga
     1741 gctggtgagc gtgacggacg agcaactggc tccgctcaga ttcgagagct atcgggaccg
     1801 gcactaccgg tccttcaaga tctggctgcc cggcgcgcag gacacgacga aaacgctcat
     1861 cctgacctac aaagtctcga acggcctcaa gtatttcgag gaccatgacg aactctactg
     1921 gaacgtcacc ggtgacgaat gggacgtgcc gatcgagtcg gcagcggcca gaattctgtt
     1981 gccccccgga gcgaccggcg tcaaagccct cgccttcagc ggcgcctacg gcgctcgcga
     2041 gcaacaggcc gacgtgcgga tcacgggccc cgaagtcctc taccggatga cgcgcccgct
     2101 cggcttccgc gaagggctca ccgccgtggt cggatgggat aaggggttcg tcgccgagcc
     2161 gggcccgctg cagctcacca aactatttct tcgatcaaac tggccgttgg ccctgccgat
     2221 cggcgtcttc ggcctgatgt ggtatctctg gtatacgcgc ggacgcgacc cgcgtctgcg
     2281 cccgattacg gtggcctacg aaccgcccga tacgttgacg ccggccgagc ttggcacgct
     2341 catcgacaac tcgccggatc tccgggacat caccgccacg ttagtggatc tcgcggtccg
     2401 cggatttctg cggatcgaag aacggcagga atccaagctc ctcggattct ggtcgagttc
     2461 ggccttttat ctgcatcggc tcaagaccgc ttcggaatgg accgacctga agccgcacga
     2521 gcgggcgatc atgaacggga tcttctcaga cgagaccacc acggtcgtcg ccctgtccga
     2581 cttggaaaat cgtttctacg cccacctgga tgggatcaag tcctccctct tcgaacaact
     2641 actgagccgc ggctactatg cccgacgacc ggaccgggtg aaacaggtct acctcaccgt
     2701 cggcatcatc gtggccatcg cgtcgatctt tggagccttc tggctgagcg aacactatgg
     2761 tattgcgctg caaaccggcc tcgctgccgg catcctctcc ggactgatca ttgtcgggtt
     2821 cggatggttc atgcccgccc ggacgatgcg cggcgcgcgg gcattggaga aagtcctggg
     2881 cttcgaggag ttcctgaccc gcgtcgaatc cgatcgtttt cagcgagtcg tcaagacgcc
     2941 ccagatgttc gagaagtttc taccgttcgc gatggccttg ggcgtggagc agaattgggc
     3001 ccgcgcgttc gacggcattt acacgcagcc ccccgcctgg tatcaagggg ccgacatcgt
     3061 cgactttcgt ccacgaagct ttaccggcaa cttgtctcag atgtccgcgg cggcgggcgc
     3121 cgtcatgacc tccgcccccc ggagcacggg tgcatcggga ttcagcagca gcgggtcatc
     3181 cggaggattc tccggaggcg gattcggcgg cggcggcgga agcggattct aacaattcga
     3241 ttcactctcc ctatgcagca gagttcacct actcatagcc tcatcggctg gatcggcgcc
     3301 ggcatgatgg gtgtgtccat gtgcggtctg ctcctgagtg gttgcgccag c
//
LOCUS       DATIFQ010000418         4516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2438974, whole genome shotgun sequence.
ACCESSION   DATIFQ010000418 DATIFQ010000000
VERSION     DATIFQ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2438974"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VLE22_21045"
     CDS             <1..1635
                     /locus_tag="VLE22_21045"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HSB16952.1"
                     /translation="TLDAEASFFVHLRLSAILGALILTGMPAVPRQISIIPQPVTVTG
                     MEGEFILKPDTVVVTDKAAATVARRFTADIAAATGYKLKIAEAGQARDNSIMLGIDNS
                     LGKLGAEGYRLEVTSKRVTVRAPKPAGVFYGTQSIRQLLPPAVYGKASSGVTWSMPCV
                     RIEDYPRFGWRGGLIDVGRHYMPKEALLRFIDVLAMHKMNSIQFHFTDDQGWRVEVKK
                     YPKLTEIGSRRKETIVGDHRNSTKYDGIPHAGYYTQADLREIVAYAAERFINVVPEIE
                     MPGHAQAAIAAYPELGNTGEKLEVRTTWGVNKNVFNANESTILFLQDVLKEMMAIFPS
                     KFIHVGGDEVPKDQWKASAEAQARMKALGLKNEEELQSYFIRRMDQFLTANGRRLVGW
                     DEILEGGLASGATVMSWRGIKGGIAAARAGHDVVMAPTTHTYFDYYQAKTGEPLALGG
                     FLPLDKVYEFEPIPPELTADEGRRVLGAQGQLWTEYINNPEYLEYMAFPRMSALAEVV
                     WTPKARKDYTAFLARMRVQEERLRARGVNFRALDKK"
     gene            complement(1715..2386)
                     /locus_tag="VLE22_21050"
                     /pseudo
     CDS             complement(1715..2386)
                     /locus_tag="VLE22_21050"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(2396..2521)
                     /locus_tag="VLE22_21055"
     CDS             complement(2396..2521)
                     /locus_tag="VLE22_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16953.1"
                     /translation="MLDHLDTLIAFTVVMTVVSLMITLLVQMVSAILGSGLRRTR"
     gene            complement(2633..3358)
                     /locus_tag="VLE22_21060"
     CDS             complement(2633..3358)
                     /locus_tag="VLE22_21060"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB16954.1"
                     /translation="MLRVLAFTDEVVVAAGLRQILTRKNGFRLVGVSDSPSNLVEMAL
                     SLRPDLMLLDFTPELTFGVLYELRERAPDRRMVLWVRSISPELAHQAVDNGVRGIVRK
                     TLPEENLRKCLRLVADGGMWFEESLKEGFFASRPINLSRRESQLVSLLSQGLKNKEIA
                     TLLLISEGSVKVYLSRLFRKLGVNDRFELALYGLRNLPHAEAALDTKPRTPGDAAGSR
                     KDVSRSEWLRSLVLNRPSDRVRA"
     gene            3671..>4516
                     /locus_tag="VLE22_21065"
     CDS             3671..>4516
                     /locus_tag="VLE22_21065"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HSB16955.1"
                     /translation="MFGTKRDTRFRLRCQRGQVLVMVTLVLIGMCGLLGLAVDLGWSY
                     FMKKSAQAAADAAALAAAEAALSTLPAVGPYNCSQATCTSGPVSCAGVASGTNLEVGC
                     QYAAQNGFAANGKDGRQNVMIEAGTTSPPPTAPGVQTHYWVTVRVYEAIPQLFSSVLG
                     NTTGAASARATAAVAHSAGMGSLILINRQNDCIPMEGGSQLACGVDLLVQANDNQGRY
                     ALQADGGILMASTSNGAEKNGRYAGENQGGGTVNAPFTFIREEGWYTNGGSSNWFQTP
                     VNRKPD"
BASE COUNT          974 a         1372 c         1402 g          768 t
ORIGIN      
        1 acactcgacg cggaggcttc ttttttcgtg cacttgcgac tttcagccat actgggcgcg
       61 ttgatcctga ccgggatgcc ggcggtgccc cggcagattt ccatcattcc gcagccggtc
      121 acggtgacgg ggatggaagg agagttcatc ctgaaaccag acaccgtggt agtgacggac
      181 aaggccgcgg cgacggtcgc ccggcgcttc acggccgaca tcgcagccgc cacaggctac
      241 aagctgaaga ttgccgaagc cggccaggcg cgggacaaca gcatcatgct ggggatcgat
      301 aactcgctcg gcaaacttgg cgccgagggc tatcggctgg aagtcacctc caagcgcgtg
      361 acggttcgcg ccccgaaacc cgccggcgtt ttctacggga cccagtcgat tcgccagttg
      421 cttcctcccg ccgtttacgg aaaagcttcc agcggcgtca cctggagcat gccgtgcgtc
      481 cggatcgagg attatccccg cttcggctgg cgtggcgggc tcatcgacgt cggcaggcac
      541 tacatgccga aagaggcgct gctccggttc atcgacgtgc tggccatgca caagatgaac
      601 tcgattcagt tccacttcac cgacgatcag ggctggcgcg tggaggtcaa gaaatacccc
      661 aagctgacgg agatcggctc gcgccgcaaa gagacgatcg tcggcgacca caggaacagc
      721 acgaaatacg acgggatccc gcacgccggt tactacacgc aggccgatct ccgggaaatc
      781 gtcgcctacg ccgccgagcg gttcatcaac gtcgtgcctg agatcgagat gccgggacac
      841 gcacaggccg ccatagccgc ctacccggag ctcggcaaca ccggcgagaa actcgaggtc
      901 cgtaccacct ggggtgtcaa caagaacgtc ttcaatgcaa acgaaagcac gattctgttc
      961 ctccaggacg tcctcaagga gatgatggcg atttttccga gcaagttcat tcacgtcggt
     1021 ggcgacgagg tgccgaagga ccagtggaag gccagcgccg aggcgcaggc ccgcatgaag
     1081 gcactgggac tgaagaacga agaagaactc cagagctact tcatccggcg aatggaccag
     1141 ttcctcaccg cgaacggacg gcggctcgtc ggctgggacg agatcctcga gggcgggctg
     1201 gcttcgggcg cgaccgtgat gtcgtggcgt ggaattaaag gcggcatcgc cgccgcgcgc
     1261 gcgggccacg acgtggtgat ggccccgaca acccacacct acttcgacta ctatcaggcg
     1321 aaaaccggag agccgctcgc gctcggcggt tttctgccac tcgacaaggt gtacgagttt
     1381 gaaccgattc cgcctgagtt gacggccgac gagggtcgcc gggtcctcgg ggcgcagggc
     1441 cagctctgga ccgagtacat caacaaccct gaatacctgg agtacatggc gttcccgcga
     1501 atgtcggcgc ttgccgaggt ggtctggacg ccgaaggcgc gcaaggatta cacggcgttc
     1561 ctcgctcgca tgcgtgtcca ggaggaacgg ctgcgcgccc gcggcgtgaa cttccgcgcg
     1621 ctggacaaga agtagcggcc cggacgatcg gcgctctcag cggaaaagga gcagggtcga
     1681 aaccgccgtc agcgccacga caaaggatct cgaacagcgg ctgcggaatg tggtccacga
     1741 gacggcgtcc ggttaccgcg cccgcgacca ccactggaac catcagcgca ttgaaagcca
     1801 gcgactgcgc gctgagcagc ccatgccacg cgtaaatggg caccttggcg agattgatca
     1861 cgaagaagaa ccacgcgccc gtggcgatga actcttcctt gggtagccgc ttgctgagaa
     1921 ggtagaggtt catcaccgga ccggcggcgt tggctaccgt agtggcgaaa ccggcggtga
     1981 caccgtatgg aaccgggtga gcagggatgt gcgagttttc cggctgcgcg cgccgtctta
     2041 ggcagacgcc gagcatggcc agtacgatgg cggccaccac gattctcagt acccgctcgc
     2101 caagtccgag cgccaccgca cccatcgcca ttccgccgag cacccagggg gcgagcgaaa
     2161 acagccgcca ggctgccgcg tggcgcctcc agtaggccac cgcaaagaca tcgccagcgc
     2221 acagcaccgg cagcagccat ccggcggact gccgggcatc cccgaccgcc agcaccatca
     2281 gaggaacgac tagaatgccg agtcccggga cgccggtctt ggcgacgccg acgaagaacg
     2341 cacacacggc gccgagcacc aactggagga ccgagaaatg cggcatcccg gtatcttagc
     2401 gggttcgtct aagtccgctt cccaaaatcg cggacaccat ttgcacgagc agcgtgatca
     2461 tcaggctgac aacggtcatc acgacggtga aagcgatcaa cgtatcgagg tggtcaagca
     2521 tagggttccc caatttggaa tcagtgtaac cccgggaggc gaagccttta gatgggcaca
     2581 gccgctccgt ctcacttccc gtggcgtgcc ggattatggt ggtgcactcg ggctaggccc
     2641 gcacgcgatc cgaaggccgg tttagaacga gcgaacgaag ccattccgag cggctcacat
     2701 cttttcttga gccagcggcg tctccaggcg tgcggggctt ggtgtcgagc gcggcctccg
     2761 catgtggcag gttccgcagg ccgtacagtg cgagctcgaa acggtcgttc acgcccaact
     2821 tgcggaaaag gcgagagagg tacactttga cggacccctc ggagatcagc agcagcgtcg
     2881 cgatctcctt gttcttcaaa ccctgcgaga ggagagagac caactggctt tcgcgcctgg
     2941 acaggttgat ggggcgtgac gcaaaaaacc cttctttcag gctctcctcg aaccacatgc
     3001 ccccgtcggc aaccagacgg agacacttcc gcaagttctc ttccggaagg gtcttgcgca
     3061 cgatgccgcg gacgccgttg tcgaccgcct ggtgtgcgag ttcgggggaa atactacgca
     3121 cccagagaac catccgtcgg tccggggccc gctcccgaag ctcgtagagc acgccgaacg
     3181 tcaattcagg cgtgaagtcc agcagcatca ggtcaggcct cagggaaagt gccatctcga
     3241 caaggttcga ggggctgtcc gacacaccga ccaacctgaa cccgttcttc cgggtcaaaa
     3301 tctgtctgag acctgcggca acgactactt catcggtaaa cgccagtact ctcaacatga
     3361 atttcctcca ttggaggctg ccctttgtgc cggtcgcggc cgacatggat aactgtccgc
     3421 cattctgcat cagccgaaac ccagcggcac gtgtttagaa taataccgca ctgcggcgct
     3481 ggaaagacac aaattcttcc agtacgtttt gtatccatgg tactagagcc actgggactc
     3541 ggtagctatg acagaatccc cgctgcggtc cattcacaca ttttgttgac agacagacac
     3601 ttggtttatc aggcgactag ccctacgagg tagtaaggcc agtgccgaaa caacactcgc
     3661 aggaggaacg atgttcggaa caaagagaga cacaagattc agattgagat gccagcgcgg
     3721 acaggttttg gtaatggtca ctctggtact cataggaatg tgcggtctgt tgggcctggc
     3781 agtggatctg gggtggtcgt atttcatgaa gaaatcggcc caggcagcgg cggatgcggc
     3841 tgcactggcg gctgcggagg ccgcgttgag cacccttcca gcggtcggcc cttacaactg
     3901 ttctcaggcg acctgcactt cgggaccagt ctcttgcgcg ggtgtagcgt ccggtaccaa
     3961 cttagaggtc gggtgccagt atgctgcgca gaacggattc gcggccaacg gcaaagacgg
     4021 ccggcaaaac gtgatgatcg aagccggaac gacttctccc ccgccgacgg cgccgggcgt
     4081 ccagacgcac tactgggtaa cggtgcgcgt gtatgaggcg attccgcaac tgttttcgtc
     4141 ggttctgggt aacacaacgg gggcggcgag cgcccgggcg acggcggcgg tcgcacactc
     4201 ggccgggatg ggttcactca tcctgataaa ccgccagaac gactgcattc cgatggaggg
     4261 tggaagtcag cttgcgtgcg gcgtggatct attggtgcaa gcgaacgata accagggcag
     4321 gtacgcgctt caggcagacg gcgggatact catggcctcc accagcaacg gagccgaaaa
     4381 gaacggacgc tacgccggcg agaaccaggg cggcggcacc gtgaacgcac cattcacctt
     4441 catccgtgaa gaaggctggt acacaaacgg cggcagttcg aactggtttc agacaccggt
     4501 caatcgtaaa cccgac
//
LOCUS       DATIFQ010000419         2304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_716867, whole genome shotgun sequence.
ACCESSION   DATIFQ010000419 DATIFQ010000000
VERSION     DATIFQ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716867"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VLE22_21070"
     CDS             complement(<1..527)
                     /locus_tag="VLE22_21070"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSB16956.1"
                     /translation="MPGCPAAPQTEVLDVLADLRGRFAVALDLGGTKLAAALIDADGR
                     IFHKRKAPTERSDTRRSVRQLVDAARKVVNEAACPWEAVAAVGLDVPGIYFAETGEVW
                     APNLPGWDRLPVRDELRRELPVPVVVDSDRAAYVLGEQWMGVAQGISDVIFLAVGTGI
                     GAGILSGGRLIRGAG"
     gene            677..1102
                     /locus_tag="VLE22_21075"
     CDS             677..1102
                     /locus_tag="VLE22_21075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSB16957.1"
                     /translation="MTTSVKGEYALQAVFDLAMFERKGPIKISDIAKRQRIPQKFLEL
                     ILAGLKQGGFVESRRGAEGGYLLARPAETITTGEVLRFVEVGRGGKHRSRRRSETPFS
                     EMWHKVDEAVSAIIDHTTFAELVRAWNDKQYKHVPNWEI"
     gene            1123..2079
                     /gene="cysK"
                     /locus_tag="VLE22_21080"
     CDS             1123..2079
                     /gene="cysK"
                     /locus_tag="VLE22_21080"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HSB16958.1"
                     /translation="MIYQDNSLSIGRTPLVKLNRVTDGAGATVLAKIEGRNPAYSVKC
                     RIGASMIWDAEKRGVLKPGKEIIEPTSGNTGIALAFVAAARGYPITLTMPESMSIERR
                     KVLKVFGAKLVLTEASGGMKAAIAKAEEIVASDPDRYVLPQQFKNPANPRIHFETTGP
                     EIWDDTDGAVDVLVSGVGTGGTITGVSRYIKQVKGKKILSVAVEPAASPVITQTLNGQ
                     PLQPSPHKIQGIGAGFVPDILDLSLVDRVELVTNEESIEMARRLSREEGIMSGISCGA
                     AAAAAVRLAKDPEFKGKTIVTVLPDSGERYLTTVLYDGLFAE"
BASE COUNT          459 a          703 c          745 g          397 t
ORIGIN      
        1 tcccccgctc cgcggatcag ccggccgccg ctcaagatgc cggcaccgat gccggtgccg
       61 accgccagga agatcacgtc cgagatgccc tgggccacgc ccatccactg ttcgccgagg
      121 acgtacgcgg cgcggtcgct atcgaccact accggaaccg gcagctcgcg tctcaattcg
      181 tcccgcaccg gaagccggtc ccacccgggc agattcggcg cccagacctc gcctgtctcg
      241 gcgaaataga tgccgggcac atcgagtccc acggcggcga ccgcttccca tgggcacgcg
      301 gcctcgttca ccaccttccg cgctgcgtcc acaagctgcc gaaccgaacg gcgagtatcg
      361 gaccgctcgg tgggggcctt cctcttgtga aagatcctgc cgtccgcatc aatcaaggcg
      421 gcggcgagct tggtgccgcc aaggtcgaga gcgacggcga acctgccgcg aaggtccgcc
      481 agcacgtcga gaacttcagt ttgaggcgcg gccgggcagc cgggcatatg cgtagtatcg
      541 cctctctttc accccagata aagggggtga aaccggcctt tcggtttttg ccgcaccgca
      601 aggacgcgat gcttgacggc gggcggggga ttcatctaat ctctagttag atacaggaga
      661 ttcctcaaca gcttccatga ccacatcggt aaagggcgaa tacgcccttc aagccgtctt
      721 tgacctggcg atgttcgagc ggaaaggacc catcaagatt tccgacatcg ccaaacgcca
      781 acgcattccg cagaaattcc tggaattgat ccttgccggg ctgaaacagg ggggattcgt
      841 cgaatcgcgg cgcggcgccg agggaggtta cctgctggcc cgcccggcgg agacgatcac
      901 cacgggagaa gtgctgcgct ttgtcgaggt gggccggggc ggaaagcatc gctcgcggag
      961 gcgatccgag acgccgttct ccgaaatgtg gcataaagtt gacgaggccg tgtccgccat
     1021 cattgaccat acgacgttcg cggagcttgt gcgcgcgtgg aacgacaagc aatacaagca
     1081 tgttccgaac tgggaaatct gattgaaccg gagagacgca gagtgatcta tcaggacaat
     1141 tcgttgagca tcggccgcac gccgcttgtg aaattaaacc gcgtgacgga cggggcgggc
     1201 gcgaccgtgc tcgccaagat cgaaggacgt aacccggcgt attcggtgaa atgccgcatc
     1261 ggcgcctcga tgatttggga cgccgagaag cgcggcgtgc taaagcccgg caaggagatt
     1321 atcgagccga cgagcgggaa cacgggcatc gctctggcgt tcgtggccgc ggcccggggt
     1381 tacccgatta cgctgaccat gccggaaagc atgtcgatcg agcggcgcaa ggtgctcaag
     1441 gtattcggcg cgaagctggt gctgaccgag gcgtcggggg gcatgaaggc cgccatcgcg
     1501 aaggccgagg agatcgtcgc ctcggacccc gaccggtacg tgctgcccca gcaattcaag
     1561 aatccggcaa acccgaggat tcatttcgag acgaccggtc cggaaatctg ggacgacacg
     1621 gatggggcgg tggacgttct cgtctccggt gtcggcaccg gcggaacgat caccggcgtc
     1681 tcgcgctaca tcaagcaggt gaagggcaag aagatcctct ccgtggcggt ggaaccggcc
     1741 gccagccctg tgatcacaca gacgctgaac ggacagccgc ttcagccgtc gccccacaaa
     1801 atccagggga tcggcgcggg gttcgttccc gacatactcg atctgtcttt ggtcgaccgg
     1861 gtggagctgg tgacgaacga agagtccatc gagatggcgc gccggctctc acgcgaagag
     1921 gggatcatgt ccggtatctc ctgcggcgcg gcggccgcgg cggcggtgcg tctcgcaaag
     1981 gatcccgagt tcaaaggcaa gacgatcgtg acggtcttgc cggattccgg cgagcggtac
     2041 ctgacgacgg ttctgtacga cgggctgttc gcggagtagg gaacggggaa caggccgcgg
     2101 tgtgctatcc gggacatggg taacagaagt tgctaaccac atgggttaca ctttttcctg
     2161 ttcctcatca tcatgatgct cagtcattgg tcgccgcgca aggagcccgg tgggtgactg
     2221 gagcggcggc caatgactgt cgatcttact cgccgctcgg ctcgggcctt ctcgttcgtc
     2281 caacagaccc agcaacaccg gccc
//
LOCUS       DATIFQ010000420         9195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_908128, whole genome shotgun sequence.
ACCESSION   DATIFQ010000420 DATIFQ010000000
VERSION     DATIFQ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908128"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /locus_tag="VLE22_21085"
     CDS             <1..1867
                     /locus_tag="VLE22_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16959.1"
                     /translation="GGQATEFRFGRVSGVTSPKLITVPYITYGGIIPRVLMSGSADTP
                     MFTSIWFDWYRTNASEPFCVKDPKATADGAEINGSMRYIAKTNGERNNLYERIFVTAS
                     PFYEETLPTIANPPSLRQEEGKQVLWTVTEPETFQADHKRCRQIAAYGLDKIMQHSHE
                     VTWRDEGDSNTMKLRASPQKGGDAMLKWYIKAQNDLGWLQGVYSNYTDFCTVNTNWNP
                     DHVQRQPDGEWRRAWPRNYALKPSKSVEFDEYYAKRIKEKFGVRMSYTDVHSAVSPWG
                     YCDYDARVPGAGTLAATFYAYGQLLINDQKVYGPTQSEATYQWLYAGLASGHYGWVYT
                     PVNLLTHPLDVAFMAHKIHPIESEYGMGYTYYYLSQLDKDWLTSPKKREYVDLFLATT
                     IGYGNMGWLVNDFGLDNTFGIEAMARSYYMMQQLQQQYAFVRPKTIEYSDRTGKFVTA
                     SLAHSTGVIADSRLHVEYENGTHVYVNRGTQGTWSMKDGEGKSLELPVNGWLVYNAKN
                     GFYEVSANVSGRRIDHVKAAEFEFLDGRGQWTERGSLGAAGSVARRDRTAGVVELIDI
                     YGNDRIAFEAKVPGAVMAYDPEGKNLGKVDAKPVRSGWYEFKPLPNGRKYVFAGE"
     gene            1920..4916
                     /locus_tag="VLE22_21090"
     CDS             1920..4916
                     /locus_tag="VLE22_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16960.1"
                     /translation="MRIAVVCLLVAGFSFAQAPDKTVPAVNEIPMWEFDRGTVLEQVG
                     QQPYEMTWVQRREDPRTLVDFEDLTGWKLELYGGAAGELHRTREQQMWGQYVARILYS
                     NASPKARVIARPPKPIAIPGKFDSVDIWGYADRRRQRTGAGTPTHISILVRDARDREH
                     RIHLNDVEWMHWWLVHRRVPADIQKQIVWPASFSGIEIEMETPNPKERYFYCDSLAFY
                     IEELRPVALKPQPKRNLKPFRGQIAGLNTGEGTLPFPTREETILPANFERDFQTTVRE
                     VSKNHFQLTYKGRDATITYDYKPRTGNLSELTASVDGGPAFRVMENGTVYFGDGEEGR
                     VPPTGDLVSATYRGDSIQARFRYGSRIVDYELRLWQKSVVLDVWCDGGEAAGLSFGRV
                     SGVKNPKLILIPYITYGASNPRVLMSGVESKPVFTSVWWDWYRTNASEPYYEREPKPA
                     ADGASINGGLRYIPKTDGQRNNLYERIFVTVSPTYEETLPTIANPPSLRQEEGKRVVW
                     TVTAPSTFQSDHERSRRVRSYGLDRIMQHSHEVTWRDHADSFTMRIHAAPQKGGDEML
                     KWYIKAQKDLGWTQGVYTNYCDFAPVNTNWDPDSVQRMPDGEWRRAWWRNYALKPARA
                     VELDEYYAKRIKERYDNVMSYTDVHTAVSPWRYCDFDARVPGAGTMAATFYAYGQLLL
                     NDQRVYGPTQSEGTYQWLYAGLTSGSYGWVYTDVNLLTHPLDVAFMLHKLHPLECDYG
                     MGSTEYYLARLDEKWAESPKLREYLDLSLATTIAYGNMGWLGTVFSQKDSFNPEALAR
                     SYYMMQQAQQQYAFVRPKTIEYAGPDGKMFTPSQAHANGVIAQSRIHVEYENGTHVYV
                     NRGKEGVWTVKTVDLPVNGWLVYNTDNGFREVSANASGHRIDHVKAPEYEFLDGRGQW
                     TERGSLGATGSVARRDRAADVVELIDIYGNDRIAFEAKASGTLMAYDSEGRSLGPVEM
                     KSPRSGWSEFKPVSNGRTYVFAKTAE"
     gene            5047..8016
                     /locus_tag="VLE22_21095"
     CDS             5047..8016
                     /locus_tag="VLE22_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16961.1"
                     /translation="MRLIITLVVLLCLLLPASAATPDNVVPAINDIERWQQVGQQPYE
                     FTWVQREEHPKTLVDFEDLKGWKLELYDGAKGEFRRSREQQMWGQYVAKFLYTGASEK
                     SRVVARPPQPIPIPEKFDSIELWGYGNRWSWVADPTTPAADVSILITDSRGQEFNIQL
                     TDIRWKQWWLIHRKLPADVFKKIVWPASFTGIEIAKGSNAEPRYLFCDSLAFFTEELK
                     PLTFKAQPKRNLKSFRGQNAGLNTGEDTLPFPTREETILPVNFEKEYKVTARQAAPGR
                     FEFRYEGKDGTVVYTYQPKTGSLSELTAGVNGAGAFRPLDGGGIRFADTPEGRISEGQ
                     LVSATLNGDVVAAKFRHGSRTVDYELRLWQKSLVLDVWCDGGDATELTFGRVTGVKNP
                     KLITIPYLTYRDTNPRVLMSGGAAGSVFTGIWFDWYRTNASEAWSAKEPQVTATSAEI
                     NGGMRYLPKTDGRRNDLYDRIFITSSPVYEETLPTTANPPSLRQEEGKSVVWTVASPR
                     TFQSDHERSRRIRSYGLDKIMQHSHEVTWRDEGDSNTMKLHAAPQKGGDAVLKWYIKA
                     QNDLGWLQGVYSNYTDFCTVNTNWSPDHVLRLSDGEWRRAWPRNYMLKPAKAVEFDEY
                     YAKRIKEKFGVRMSYTDVHTCAQPWRCDYDVRVPGAGTMSSLFYAFGQLLLNDQKAYG
                     PTQSEATFQWLYAGLQSGGYGWVYTDVNLLTHPLDVAFMLHKIHPLECDYGMGYTNYY
                     LDRMDKEWRKSPKKREYVDLFLATTIGYGNMGWLVNDFGLDNAFGIEAMARSYYMMQQ
                     LQQQYAFVRPKRIEYADGGGKMLSPSQAQATGAINDSRLHVEYENGTHVYVNRGKEGV
                     WRVKTIDLPVNGWLVYSSGFYEISANVSGHRIDHVKSAEYEFLDGRGQWTEHGSLGAT
                     GSVARRDRAADVVELIDIYGNDRILFQAKASGTLMAYDWEGKSLGKVDTSSPKAGWHE
                     FKPIKDGRMYVFGRNGD"
     gene            8118..>9195
                     /locus_tag="VLE22_21100"
     CDS             8118..>9195
                     /locus_tag="VLE22_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16962.1"
                     /translation="MRLIIAAVVLLYSILPATGATPDKVVPAINEIELWQQVGQQPYE
                     FTFTQREEHPKTLVDFEELKDWNLELYDGAKGEFRRSREQQLWGQYVAKFLFSGASDK
                     SRMIARPPKPIPIPERFDSVEFWGYANRWGWMRDETTPPVDVSILIQDARGKEFTVSM
                     TDTRWKQWWLIHRKLPAAIAKEIVWPATFTGIEIAKLKNADPRYFFCDSLAFYMEDLK
                     PVPLHRQPKRNLKPFRGQITGLNTGEGTLPFPTREETILPANFEKDFQVTARQAAPNR
                     FELQYKGKDATVTYTYQPKTGSLSELTVAVNAGTPYRPMDGGGVRFTDTAEGQVPQGS
                     VLSATLQDGVVKAKFAIGSRVVDYE"
BASE COUNT         1953 a         2886 c         2707 g         1649 t
ORIGIN      
        1 cggaggccag gccacggagt tccgatttgg tcgggtgtct ggtgtaacga gcccgaagct
       61 gatcactgtg ccttacatca cgtacggggg aatcattccg cgcgtgctga tgtccggcag
      121 cgcggacacg ccgatgttca cctcgatctg gtttgactgg taccgcacca acgcgtcgga
      181 gccgttctgt gtgaaggatc ccaaagccac cgcggacggc gccgagatca acggcagcat
      241 gcgctacatc gcgaaaacga acggcgaacg caataacctg tacgaacgta tcttcgtcac
      301 cgcgtccccg ttctatgagg aaacgttgcc gaccatcgcc aatccgcctt cgctccggca
      361 ggaggagggc aaacaggttc tttggacggt gaccgaaccc gaaaccttcc aggctgatca
      421 taaacgctgc cgccagatcg cggcgtacgg cctcgacaag atcatgcagc acagccatga
      481 ggtcacctgg cgtgacgaag gcgacagcaa caccatgaag ctgcgtgcgt caccgcaaaa
      541 gggcggcgac gcgatgctga agtggtacat caaggcgcag aacgatctcg gttggcttca
      601 gggggtctac agcaactaca ccgacttctg cacagtgaac accaactgga acccggacca
      661 cgtccagcgg cagccggacg gcgaatggcg ccgcgcctgg ccccgaaact atgctctgaa
      721 accttcgaag tcggtcgagt tcgacgagta ctacgcaaag cggatcaagg aaaagttcgg
      781 cgtgcggatg tcctacaccg acgtccatag cgccgtttcg ccgtggggct attgcgacta
      841 cgacgcccgt gtcccgggcg ctggcacgct ggccgcgacg ttctacgctt acggccagct
      901 cctgatcaat gaccagaaag tctatggacc gacccagtcc gaagcgacct accagtggct
      961 gtacgctggg cttgcctcgg gccactatgg ctgggtctac acaccggtga acctgctcac
     1021 ccatcctctc gatgtggcct tcatggcgca caagattcat cccatcgaaa gcgagtacgg
     1081 gatgggttac acgtattact acctctcaca gctcgacaag gactggctga cgtcgccgaa
     1141 aaaacgcgag tacgtggacc tgttcctggc aaccaccatc ggctacggca acatgggctg
     1201 gcttgtcaac gactttggtc tggacaacac gttcggcatc gaagcgatgg cccggtcgta
     1261 ctacatgatg cagcaactcc agcagcagta cgccttcgtt cgccccaaga ccatcgagta
     1321 ctccgatcgg acgggcaagt tcgtcacggc cagcctggcc cactccaccg gcgtgatcgc
     1381 cgactcccgc ctccatgtgg agtatgaaaa cggcactcac gtctacgtga accgtgggac
     1441 ccaagggacc tggagcatga aggacggaga aggaaaatct ctcgagcttc cggtcaacgg
     1501 ctggctcgtc tacaacgcga aaaacggctt ttacgaagtc tcagcgaatg tgtccgggcg
     1561 ccgcatcgac cacgtgaagg cggccgagtt cgaattccta gacggccgcg gccagtggac
     1621 cgaacggggc tcgctcggtg cggcgggcag cgttgcgcgc cgcgatcgca ccgccggcgt
     1681 cgttgaactg atcgacatct acggcaacga ccgcatcgcg ttcgaggcga aagtgcccgg
     1741 agccgtgatg gcctacgatc cggaaggcaa gaacctcggc aaggtagatg cgaagccggt
     1801 ccggtctggc tggtacgagt tcaagccttt gcccaacggc cgcaagtacg ttttcgccgg
     1861 cgagtagcgg actatacacg agcctgtctt ttttgatatg aattgctacc gacatgcgga
     1921 tgcgaatagc tgttgtatgc cttctggtcg ccgggttttc attcgctcag gcgcccgata
     1981 aaaccgtccc cgccgtcaac gagatcccca tgtgggaatt cgaccgcggc accgtcctcg
     2041 aacaagtggg gcagcagccc tacgagatga cgtgggtcca acgccgcgaa gacccgcgaa
     2101 cactggtcga tttcgaagac ctcactggct ggaagctgga gttgtacggc ggggccgccg
     2161 gcgaactcca ccgcacgcgc gagcagcaga tgtggggcca gtacgtcgcc cggattctct
     2221 actcgaacgc ctcgcccaaa gcccgtgtga tcgctcggcc tccgaagccc atcgcgatcc
     2281 ccggcaagtt cgactccgtc gacatctggg gttacgccga ccgccggcgc caacgcaccg
     2341 gagccggcac gccgacgcac atctcgatcc tcgtgcgtga cgcacgtgat cgcgagcacc
     2401 gcattcacct caacgacgtg gagtggatgc actggtggct ggtccaccgc cgcgttcccg
     2461 ccgacattca gaagcagatc gtgtggccgg cgagtttctc gggcattgaa atcgagatgg
     2521 agacgccgaa tccgaaagag cgctacttct attgcgattc cctggcgttc tacatcgagg
     2581 agctgaggcc tgtcgcgttg aaaccgcagc ccaagcgcaa tctgaagcca ttccgcgggc
     2641 agatcgccgg cctcaatacc ggagagggca cattgccttt ccccacgcgc gaggaaacga
     2701 ttcttccggc caactttgaa agggatttcc agactacggt ccgcgaggtt tcgaagaatc
     2761 acttccagtt gacctacaaa ggccgtgacg cgaccatcac ctacgactac aagccgcgga
     2821 ccggcaactt gagcgaactg accgccagcg tggacggagg gcccgcattc cgcgtgatgg
     2881 agaatggaac ggtctatttc ggtgacggcg aggaagggcg cgtgccgcca actggtgatc
     2941 tggtctcggc aacataccga ggcgactcga tccaagcaag atttcgctac ggctcgcgga
     3001 tcgtcgatta cgaattgcgt ctgtggcaga aatcggttgt ccttgacgtc tggtgcgatg
     3061 gcggcgaagc tgcgggcctg agtttcggac gcgtctccgg cgtgaagaac ccaaaactga
     3121 tcttgatccc ctacatcacg tacggcgctt cgaacccgcg cgtgttgatg tccggtgtcg
     3181 aatcaaaacc ggtcttcacg tccgtctggt gggactggta tcgcaccaac gcctccgagc
     3241 cttactatga gcgggaaccc aaacctgccg ccgacggcgc gtcgatcaac ggcgggctgc
     3301 ggtacatccc gaagaccgac ggccagcgca acaacctgta cgaacggatc tttgttaccg
     3361 tctctcccac ctacgaagag acgttgccga ccatcgccaa cccgccctcg cttcggcagg
     3421 aggagggcaa gcgggtggtc tggacagtca ccgcaccgtc caccttccag tccgatcacg
     3481 agcgcagccg ccgcgttcgc tcgtacggtc tcgacaggat catgcagcac agccatgagg
     3541 tgacctggcg cgaccatgcc gacagcttca ccatgcgcat ccacgccgct ccgcagaagg
     3601 gcggcgacga gatgctgaag tggtacatca aggcgcagaa agatctcggc tggacgcagg
     3661 gcgtctatac gaactactgc gacttcgcgc cggtgaacac gaactgggac ccggattccg
     3721 tccagcgcat gcccgacggc gaatggcgtc gtgcctggtg gcgcaactat gcgctgaagc
     3781 ccgccagggc tgtcgaactc gatgaatact acgccaaacg tatcaaggaa cgctacgaca
     3841 acgtgatgtc gtacaccgac gttcacacgg ctgtgtcccc gtggcgctac tgtgacttcg
     3901 acgcccgcgt ccccggcgcc ggcacgatgg cggcgacctt ctacgcctat ggccagcttc
     3961 tcctgaacga tcagcgcgtc tacggcccca cgcagtccga ggggacctac cagtggcttt
     4021 acgccgggct cacctcaggc agctacggct gggtttacac cgacgtcaat ctgctaaccc
     4081 atcctctcga cgttgccttc atgctgcaca agctccaccc actcgaatgc gactacggca
     4141 tgggatcgac ggagtactac ctcgcccgcc tcgacgagaa atgggctgag tcgccgaagc
     4201 tccgcgagta cctagatctc tccctcgcca ccacgattgc ctacggcaac atgggctggc
     4261 tcggcacggt cttcagccag aaagacagct tcaaccccga agccctcgcc cgctcgtact
     4321 acatgatgca gcaggcgcag cagcagtacg cctttgtccg gccgaagacc atcgagtacg
     4381 ccggcccgga cggcaagatg tttacgccca gccaggcgca cgcgaacggc gtgatcgctc
     4441 agtctcggat tcacgtcgaa tacgagaacg gcacgcacgt ctacgtgaac cgagggaaag
     4501 aaggagtctg gacggtgaaa acggtcgacc tccccgtcaa cggctggctc gtctacaaca
     4561 cggacaacgg cttccgcgag gtctcagcca acgcctccgg ccaccgcatc gatcacgtga
     4621 aagcgccgga gtacgaattc ctcgacggcc gtggacagtg gaccgagcgc ggttctcttg
     4681 gcgcaaccgg cagcgtcgcc cgccgcgacc gcgccgccga cgtagtcgag ctaatcgata
     4741 tctatggcaa cgaccgcatt gcatttgaag cgaaggcctc gggcactctg atggcctacg
     4801 attcggaggg caggagcctc ggtccggtcg aaatgaaatc tccgcgctca ggctggtctg
     4861 aattcaaacc agtatccaat ggccgcacgt acgtattcgc gaagaccgcg gagtgaaaac
     4921 ccgggactga ctcccatttc gccgccgttg cgcgaaaacc cgggactgac tccaaatttc
     4981 gccgttgtcg cgaaattggt gtctgtcccg ggtttccgcc actttccgaa atctccaagg
     5041 aaacttatgc gactcatcat tacactcgtt gttcttctct gcttactcct cccggcgtcc
     5101 gccgctaccc ccgacaacgt cgtccccgcc atcaacgata tcgagcgatg gcagcaggtc
     5161 ggccaacagc cctacgagtt cacctgggtc cagcgcgagg aacaccccaa gacgctcgtc
     5221 gattttgagg atctcaaggg ttggaaactg gagctctacg acggcgcaaa aggcgagttt
     5281 cgccgcagcc gtgagcagca gatgtggggc cagtacgttg cgaagttcct ctataccggg
     5341 gcgagtgaga agagccgtgt cgtcgcccgg ccgccccagc cgattcccat ccctgagaag
     5401 tttgattcga tcgaattgtg gggctacggc aaccgctggt catgggtagc cgatcccacc
     5461 actccggcgg ctgacgtctc catcctcatc accgactctc gcggccagga attcaacatc
     5521 cagctcaccg acattcgctg gaagcagtgg tggctcatcc accgcaagtt gcccgcggat
     5581 gtcttcaaga agatcgtctg gcccgcgagc ttcaccggta tcgagatcgc caagggcagc
     5641 aacgccgagc cgcgttacct cttctgcgac tcactcgcgt tcttcaccga ggagttgaag
     5701 cccctcacgt tcaaggccca gccgaagcgg aacctgaagt ccttccgcgg tcagaatgcc
     5761 ggcttgaaca ctggggagga tacgctgccc ttccccacgc gtgaggagac catcctgccg
     5821 gtcaatttcg agaaagagta caaggtgacc gcacggcagg cggccccggg acggttcgag
     5881 ttccgatacg aaggcaagga cggcaccgtc gtctacacgt atcagccgaa aacgggcagc
     5941 ctgagcgagc tcaccgccgg cgttaacggc gccggcgcgt tccgcccatt ggacggtggc
     6001 ggcatccgtt tcgcggacac gcccgagggc aggatctccg agggtcagct tgtctccgca
     6061 acgctcaacg gtgacgtggt cgcagcgaag ttccgccacg gctcacggac cgtcgactat
     6121 gagctgcgcc tctggcagaa gtcgctcgtg ctggacgtct ggtgcgacgg cggcgacgcc
     6181 accgaactga ccttcggacg tgtgaccgga gtcaagaacc cgaaactgat cacgatcccc
     6241 tacctcacct atcgcgacac caacccacgc gtgctgatgt ctggtggtgc ggcgggatcc
     6301 gtgttcaccg gaatctggtt cgactggtac cggacgaacg cgagtgaagc ctggtcggcg
     6361 aaggagccac aggttaccgc gaccagcgct gagatcaacg ggggtatgcg ctaccttccg
     6421 aagaccgacg gccggcggaa cgatctctac gaccgcatct ttatcaccag ttcgccggtc
     6481 tatgaagaga cgctgcctac caccgcgaat cctccctcgc tgcggcagga ggaaggtaag
     6541 agcgtggtct ggaccgtcgc gtcacccagg acgttccaga gtgatcatga acgctcgcgt
     6601 cggatccgct cctacggcct cgacaagatc atgcagcaca gccacgaggt cacctggcgg
     6661 gacgagggcg acagcaacac catgaaactc catgccgccc cgcaaaaggg cggcgacgcg
     6721 gtgctgaagt ggtatatcaa ggcgcagaac gatctcggtt ggctccaggg cgtctacagc
     6781 aactacaccg acttctgcac cgtgaacacg aactggagcc cggaccatgt gctccgtctt
     6841 tcggacggcg aatggcgccg cgcttggccg cgcaactaca tgctcaaacc ggccaaggca
     6901 gtagaattcg atgagtatta cgccaagcgg atcaaggaga agttcggcgt gcggatgtcg
     6961 tacaccgacg tccacacctg cgcccagccc tggcgctgcg attacgacgt ccgggtcccg
     7021 ggcgctggaa cgatgagttc gctgttctac gctttcgggc aattgctgtt gaacgatcag
     7081 aaggcgtacg gcccgacgca gtcggaggcc acgttccagt ggctctacgc cgggctgcaa
     7141 tccggcggat acggttgggt ctacaccgac gtgaacctgc tgacccaccc gctcgacgtc
     7201 gctttcatgc tgcacaagat tcatccgctc gagtgcgact acggcatggg ctacacgaac
     7261 tactatctcg atcggatgga caaggagtgg agaaaatccc cgaagaaacg cgagtacgtg
     7321 gatctgttcc tcgccacaac catcggctac ggcaacatgg gttggctggt caacgatttc
     7381 ggcctggaca acgcgtttgg tatcgaagcg atggcccgct cgtactacat gatgcagcag
     7441 cttcaacagc agtacgcgtt cgtgcggccg aaacgaattg agtacgcaga tggcggtgga
     7501 aagatgctga gtccgagcca ggcacaggcc acgggcgcaa tcaacgactc gcgcctgcat
     7561 gtcgagtacg aaaatggcac gcacgtctat gtgaaccgcg ggaaggaagg agtctggagg
     7621 gtgaagacga tcgaccttcc cgtcaacggc tggctggttt acagcagcgg tttttacgaa
     7681 atctcggcga acgtgtctgg ccatcgcatc gaccatgtca agtccgccga gtacgaattc
     7741 ctggacggcc gcggccaatg gaccgaacac ggatcgctcg gtgccacagg cagcgtggcc
     7801 cgccgcgacc gggccgctga tgtggtcgag ctaatcgata tctatggcaa cgaccgcatt
     7861 ttgtttcaag cgaaggcctc gggcactctg atggcctacg attgggaggg caagagcttg
     7921 ggcaaggttg atacgtcgtc gccaaaggcc ggctggcacg aattcaagcc catcaaagac
     7981 ggccgtatgt acgtattcgg gagaaatggg gactgaccca tttagtgccg ttggcgaaaa
     8041 cccgggattg actccaaatt tcgccgttgt tgcgaaattg gtgtttgtcc cgggttttcg
     8101 gaaacttaag gagacttatg cgactcatca ttgcagccgt tgttcttctc tattcgatcc
     8161 tgccggcgac cggggctacc ccggacaaag tcgtgccggc gatcaatgaa atcgagctct
     8221 ggcaacaggt aggccagcag ccctacgagt tcaccttcac acagcgcgag gaacatccca
     8281 agaccctcgt cgatttcgag gaactcaaag actggaacct cgagctctac gacggcgcga
     8341 agggtgagtt ccgccgcagc cgtgaacagc agttgtgggg acagtacgtc gcgaagttcc
     8401 tgttttccgg ggccagcgac aagagccgga tgatcgcccg gccgccgaag ccgatcccga
     8461 ttcccgaacg cttcgactcg gtcgagttct ggggctacgc taaccgctgg ggatggatgc
     8521 gggatgagac cacgccgccg gtcgacgtgt cgatcctgat ccaggacgcc cgcggcaagg
     8581 aattcaccgt ctcgatgacg gacacccgct ggaagcagtg gtggctcatc caccgcaagc
     8641 tgcccgccgc gatcgccaag gaaatcgtct ggccggcgac gttcacgggt atcgagatcg
     8701 ccaaactgaa gaacgccgac ccgcgatact tcttctgcga ttccctcgcc ttctacatgg
     8761 aggaccttaa gccggtcccg ctccataggc agcccaagcg gaatctgaag ccgttccgcg
     8821 ggcagatcac cggcctcaac accggcgaag gcacgctgcc tttcccgact cgcgaggaaa
     8881 ccatccttcc agccaatttc gagaaggact tccaggtgac cgctcgccag gccgccccga
     8941 accgtttcga gcttcagtac aagggcaagg atgccaccgt cacctacacg tatcagccaa
     9001 aaaccggcag tttgagtgaa ctgacggtcg ccgtcaacgc cgggacgccg taccggccga
     9061 tggacggagg cggggtccgc ttcaccgaca ctgccgaggg gcaggtcccg caaggctccg
     9121 tactttccgc aacgcttcag gacggcgtgg tcaaggccaa gttcgcgatc ggctctcgtg
     9181 tcgttgacta tgagc
//
LOCUS       DATIFQ010000421         2437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_334260, whole genome shotgun sequence.
ACCESSION   DATIFQ010000421 DATIFQ010000000
VERSION     DATIFQ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334260"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VLE22_21105"
     CDS             <1..380
                     /locus_tag="VLE22_21105"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HSB16963.1"
                     /translation="AMKTKNTTPTTQELEIMKVVWALGPATVRDVYEALRRRRKVAYT
                     TVMTMMNILEQKGHLKKKRGDKAYVYQAAKPRKQVVRNMVEEFLGRVFDGSARPLLVH
                     LVEGRRLSADDLRDIERMMEEGE"
     gene            385..1620
                     /locus_tag="VLE22_21110"
     CDS             385..1620
                     /locus_tag="VLE22_21110"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HSB16964.1"
                     /translation="MAEFDNLISYCFQLAVLAAAGLVMPFFLRLHAPRTVHGYLQAVL
                     ALCLLLPAVQPWREIRTATAVSLQTRTPAETGREAEIPALDWSETILVVLLAGVVLRL
                     LWLAVGLWRLRMYRRRSRPIEDLPPGILQMQARLRVFPRFYGSAEVGGPVTFGLFRPV
                     VLVPERFLTMDAESQEAVACHELLHVRRRDWAITLAEEVIRSLFWFHPPLLWLIERIR
                     ATREQIVDGLVVRWTGARRHYVQALLSMASEPARADLLAPSFARKHHLRERVALLLKE
                     SSMSKGRLTLTLAVIGLGTVSSVALAVSSFPLSRPAAADRVYRIGDGIVGPQLLSKIE
                     PEYTQEARDNKVQGTVVLYAEVSKTGKVENIKVVKGLDSGLDANAIAALKLWKFKPGT
                     KDGKPVRVAATIEMNFRLQ"
     gene            complement(1663..>2437)
                     /locus_tag="VLE22_21115"
     CDS             complement(1663..>2437)
                     /locus_tag="VLE22_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16965.1"
                     /translation="LDVLRRVPGMVCQIAAVEVRSEASVWIPAWLYLPHKQDRQKPVL
                     LVLEPGGRNRDWAEGDLYQSVAGEGYPVCAVDVRGVGDLSPEFGRHAPGYARSHNQEE
                     DYAWASLIFGRPLLGQRVTDILAVSSALRSDAALKGRRLAVAARGHLTVPALFAAALE
                     KDLDQLYLAGGLVSFQSIVETENYSHPFGNFISGLLKHTDLPEIAASVAPRRVVLAGA
                     VGADGSGLDLSVVQQAYPDASNVDILPGAKWDVETLARI"
BASE COUNT          460 a          747 c          774 g          456 t
ORIGIN      
        1 aagcgatgaa aacgaagaac accacaccta caacgcaaga actcgagatc atgaaagtcg
       61 tctgggcgct cggcccggcc accgtccggg acgtttatga ggcccttcgc cggcgccgga
      121 aagtcgccta cacgacggtt atgacgatga tgaacatcct cgagcagaaa ggccacctga
      181 agaagaagcg gggcgacaag gcttacgtct accaggccgc gaagccgcga aaacaggtgg
      241 tccggaacat ggtcgaggag ttccttggac gggtgttcga cggctccgcc cgccctctgc
      301 tggtccatct cgtcgagggc cgccggctga gcgcggacga cctgcgtgac attgagcgaa
      361 tgatggaaga aggcgagtga cgcgatggcc gaattcgaca acctgatctc ttactgtttc
      421 cagttggctg tgctggcggc cgccgggctc gtgatgccgt tcttcctgcg gttgcatgcg
      481 ccgcgcacgg tccacggcta ccttcaggcc gtgctggcac tctgcttgct gctgccggct
      541 gtgcagccgt ggcgggagat ccgcacggcg acggcagttt cactacagac gcgcacaccg
      601 gcggagaccg ggcgcgaggc cgagatccct gcactcgact ggagcgaaac gattctggtc
      661 gtgcttctcg ccggcgtagt ccttcgcctg ctctggctgg ctgtgggatt gtggcgtctt
      721 cggatgtacc ggcggcgctc ccgcccgatt gaagatctgc ctccagggat tctccagatg
      781 caggcgcgcc tccgtgtctt tccgcgcttc tatgggtcgg cggaggtcgg cggcccagtc
      841 accttcggcc tgttccggcc ggtggtgctc gttccggaga ggttcttgac gatggacgcc
      901 gagtcccagg aagcggtcgc gtgccatgaa cttctgcacg tgcgccgccg cgactgggct
      961 attacactcg ccgaagaagt tatccgatcg ctcttctggt ttcatccacc gctgttgtgg
     1021 ttgattgaac gaatccgtgc gacgagggaa cagatcgtcg acggtctggt tgtacgttgg
     1081 accggcgcac gccggcatta cgtccaggcg ctcctttcga tggcgtccga gcccgcgcgg
     1141 gccgatctgt tggcgccatc gttcgctcgc aaacaccatc tcagggagcg cgtggcgctc
     1201 ctgctcaagg agtcatccat gtcgaaaggt cgtctcacgt taacgctggc agtcattgga
     1261 ttgggcacgg tttcatccgt ggcgctggct gtgtcatctt ttcccctctc tcgtccggcg
     1321 gcggcggatc gcgtttacag gatcggggat ggcatcgtcg ggccgcaatt gcttagcaag
     1381 atcgagccgg agtacacgca agaggcacgg gacaacaagg tgcagggtac ggtcgtgctc
     1441 tacgccgagg tgtcgaagac aggcaaagtc gagaacatca aagtggtcaa gggccttgac
     1501 tcgggcctgg acgcaaacgc catcgcggcc ctcaagctgt ggaagttcaa gcccggcacg
     1561 aaggacggca aacccgtccg agtcgccgca accatcgaaa tgaacttccg gctgcagtaa
     1621 ccgccttacg cttcgcaact aagcgactgg cgggcttgcc gttcagatcc gagcgagcgt
     1681 ctcgacatcc catttcgccc ccggcaagat atccacgttg gaggcgtccg ggtatgcctg
     1741 ttgaacgacg gacagatcga gccccgaacc gtccgctccg acggcgcccg ccagcacgac
     1801 ccttcgcgga gcgacggaag ccgctatctc gggtaggtcg gtgtgcttca acagaccgga
     1861 gatgaagttg ccgaacgggt gagagtagtt ctccgtttcc acgatgctct ggaatgaaac
     1921 caacccaccg gccaggtata gctggtcgag gtctttttca agcgccgcag cgaaaagagc
     1981 gggtacggtg aggtgacctc gcgcggcgac ggccagccgg cgccctttca gagcagcgtc
     2041 ggaacgaagc gccgaggaga cagccagaat gtcggtaacg cgctgtccta gcagcggcct
     2101 cccgaagatc agcgacgccc aggcgtaatc ctcctcctga ttgtgggagc gcgcatatcc
     2161 aggcgcgtgc cggccgaact cgggggagag gtcgccgact ccgcggacgt cgaccgcaca
     2221 cacagggtaa ccttctccgg caacggattg atagagatcg ccttcagccc agtcgcgatt
     2281 acgtcctccc ggttcgagta cgagcaggac tggcttctgc ctatcctgct tgtgcggaag
     2341 gtacagccac gccgggatcc atacggaggc ttcgctccgc acttcgacgg ctgcgatctg
     2401 gcagaccatg cccggcacac ggcgaagtac atcgaga
//
LOCUS       DATIFQ010000422         4755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2058866, whole genome shotgun sequence.
ACCESSION   DATIFQ010000422 DATIFQ010000000
VERSION     DATIFQ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058866"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1199
                     /locus_tag="VLE22_21120"
     CDS             153..1199
                     /locus_tag="VLE22_21120"
                     /inference="COORDINATES: protein motif:HMM:NF018828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16966.1"
                     /translation="MTLPSTYIAALLLTILTMLCWGSWANTLKLSGKWRFELFYFDYA
                     FGVLLASVIAAYTFGTFGYDGFLFMDDLMRAGKRNMVLGFAGGVVFNLANMLLVAAIS
                     VAGLAVAFPVGIGLALVIGVIWNYVLNPQGNPTFLFLGAGLVVLAIIVDAFAYRSLEF
                     QRAEERIKAGLSRTLRPKVSMKGILLSLISGVLMGSFYPLVEMGKAGDAGLGPYAIAF
                     VFALGVFFSTFVFNLYFMNLPVQGEPVEILDYFRGTAKQHLLGIAGGLIWCAGTVANF
                     VAASAPQEIQVGPAVSYAIGQGATAVSALWGLLVWKEFAGADAKVKSLLVLMFFLFLS
                     GLALVSIAPLFASR"
     gene            complement(1201..2379)
                     /locus_tag="VLE22_21125"
     CDS             complement(1201..2379)
                     /locus_tag="VLE22_21125"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HSB16967.1"
                     /translation="MTERLYYTDSYLAEFRARVVGTDADGLRVSLDRTAFYPSSGGQP
                     NDTGTLAGVAVVDVIDEGEQIIHVTAAPMTAGEVEGRIDWRRRFDHMQQHSGQHLLSA
                     VLTDLFRIPTVSFHLGADSSTIDVAAPSLEPKQILAAEERANELVAENLPIAISFEAA
                     AAVKDLRKESGREGTLRIVSIEGHDRSACGGTHVHATGEIGPILLRGIERIRGNVRIE
                     FLCGMRAVRRARVDFDALSRIARTFSATLDDTPSLVASQFEAFQAADKTRRKLAGELT
                     RFRGRELYENTPQGPDGSRRATHRTGGPIDDEVRALAQGFTAQPGAVFLAVTDDPPSV
                     LLAASSDAGVHAGNMLKTALTKLGGRGGGSAQIAQGSVPSKEALAQLISELGMALPAM
                     "
     gene            complement(2405..2761)
                     /locus_tag="VLE22_21130"
     CDS             complement(2405..2761)
                     /locus_tag="VLE22_21130"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSB16968.1"
                     /translation="MPEGYIPIFVFLVLSILFPVVVLVAARLVRPALPSIVKLDAYEC
                     GIKAASDSRGRYTVRFYIIAILFVIFDVEAVFLFPWAVRYHKLGWFGVAEVAVFLAIL
                     IVGYIWAYKKGALEWV"
     gene            complement(2807..3928)
                     /locus_tag="VLE22_21135"
     CDS             complement(2807..3928)
                     /locus_tag="VLE22_21135"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HSB16969.1"
                     /translation="MLRRIYLDFNASTPIAPEVAHAMSPFLEGHYGNPSSTHWAGVQA
                     REAVENARGQVAGLLDCRPREIVFTSGGTEANNHAIKGVFFAKGGSGAHIITTQVEHP
                     AVISPCRFLERLGAAVTYLPVDRYGRVDPEDVRRALRPSTILISVMHANNEVGTIQPV
                     AAIARIARERDALFHTDAAQSAGKISTNVHDLGVDLLSVAGHKLYAPKGVGALFVRDG
                     VAIEPLVHGAGHESGRRAGTENVLLDVGLGAACELARLWTGGTRLRELRDLFWNLLRN
                     QFGEGVVLNGHPEERLPNTLNVSFTGRAGADILARLDGVAASTGSACHSGCVELSPVL
                     QAMQIPPEVGMGAIRFSLGRTTTREEIEETVEMLARVVR"
     gene            3990..4658
                     /gene="cysE"
                     /locus_tag="VLE22_21140"
     CDS             3990..4658
                     /gene="cysE"
                     /locus_tag="VLE22_21140"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HSB16970.1"
                     /translation="MFRTIKEQVDTIFREDPAAKSVLEILLCYPGFHAVLLHRLAHKF
                     YRWNIPVIARVMSQVSRFFTGIEIHPGARIGRRFFIDHGMGVVIGETTEIGDDVLLYQ
                     GVTLGGTGKEKGKRHPTIGNNVVIGTGAKVLGNIRIGDHVKIGAGSVVVHPVPDHSTV
                     VGVPGRVVRRATEVPESEDVLEHGRLPDPEAQAIDELAQRLEQLEAAVRMLSEDKATA
                     RRGS"
BASE COUNT          863 a         1516 c         1414 g          962 t
ORIGIN      
        1 ctggcggggc tccaaccggg agtgcgcagg gcgatacctg acagatcgtg cttcttccat
       61 tcatgttttg caggttgaga ccggcatgcc actctgcttg acctcacggc gactcgcatt
      121 atattctcgg ctttcgatcc agaggacatg ttatgaccct accgagtacc tacatcgccg
      181 ctctgctgct gaccatcctc acgatgctct gttggggatc ctgggccaac acgttgaaac
      241 tctccggcaa atggagattc gagctgttct atttcgacta tgcgttcggc gttctgctgg
      301 cctccgtcat cgccgcctat acgttcggca ctttcggcta cgatggcttc ctcttcatgg
      361 acgacttgat gcgcgccggc aagcgcaaca tggttctcgg tttcgccggt ggggttgtct
      421 tcaacctcgc caatatgctg ctggtggccg ctatctccgt ggccgggctg gcggtagcgt
      481 tcccggtggg aattggtttg gcgctggtga ttggtgtcat ctggaattac gttctcaatc
      541 cccagggcaa cccgacgttt ctgttccttg gcgcaggctt ggtggtgctg gcgatcattg
      601 tggatgcgtt cgcttaccga tctctcgaat ttcagcgggc cgaggaaagg atcaaagcgg
      661 gcctgagccg gacgctaaga cccaaggtct cgatgaaggg tattctgctg agcctgatca
      721 gcggcgtgct tatgggatcg ttctatccgc tggtggaaat gggcaaggcc ggcgatgccg
      781 gactggggcc gtatgccatc gctttcgtct tcgcgcttgg cgtgttcttc tcaaccttcg
      841 tcttcaacct ctacttcatg aatctgcccg ttcagggcga gcccgtcgag atcctcgact
      901 atttccgtgg caccgcaaag cagcatttgc tgggcatcgc cggaggactg atctggtgtg
      961 ccggaacggt cgcgaatttc gtcgctgcca gcgcgcctca ggagatccag gtcgggccgg
     1021 cggtgagtta cgccattggc cagggcgcca cggcggtcag cgcgctttgg ggactgctcg
     1081 tttggaagga gttcgccgga gccgatgcca aggtcaagtc gctgcttgtg ctgatgttct
     1141 ttttgtttct gtcgggcctt gccctggttt cgatcgcgcc gctgttcgcc agccggtagg
     1201 ttacatcgct ggcagtgcca ttcccagctc cgagataagt tgtgctaggg cttcctttga
     1261 cggcacgctg ccctgcgcta tctgcgcgct cccgccgcct cgtccgccaa gcttcgtcag
     1321 tgcggtcttc agcatgttgc ctgcatgaac gccggcgtct gaagaggcgg ccagcagtac
     1381 cgacggcggg tcgtcggtca ccgccagaaa cacggcgccc ggctgcgcgg tgaagccctg
     1441 cgcgagcgct cggacttcgt cgtcgatcgg gccacccgtt ctgtgagttg cgcgcctgct
     1501 gccgtcgggc ccctgcgggg tgttctcgta gagttcccta ccccggaaac gggtgagttc
     1561 ccctgccagt tttcgccgtg tcttgtcggc cgcctggaac gcttcaaact gcgacgccac
     1621 gagcgacggg gtgtcgtcca acgttgccga aaatgttcgg gcgattcgtg agagcgcgtc
     1681 gaaatccacc cgggcgcgcc gtaccgcccg cattccgcac aggaactcga tccggacgtt
     1741 gccacgaatt ctctcgatgc cgcgcagcag tatcgggccg atttcgccgg tcgcgtgcac
     1801 gtgcgtccca ccacaggcgc tccggtcatg cccctcaatc gacacgatcc gcagcgtacc
     1861 ttcacgaccc gattccttcc ggaggtcttt caccgccgcg gccgcttcga aactgatggc
     1921 gatcggaagg ttttcggcca ccaattcgtt ggcgcgttcc tccgctgcca ggatttgttt
     1981 cggctcgagc gaaggagcgg caacatcgat ggtggaggag tcggcgccga ggtggaagct
     2041 gacggtcggg atcctaaaaa gatcggtgag cacggccgaa agcaggtgct ggccgctgtg
     2101 ctgctgcata tggtcgaagc gccgccgcca gtcgattcgg ccctccacct caccggcggt
     2161 catcggagcc gccgtcacgt ggatgatctg ctcgccttcg tcaatcacgt ctacaacggc
     2221 tacgcccgct agcgttccgg tgtcgttcgg ctggccaccg gaagaaggat agaaggcagt
     2281 gcggtccagg gacacccgga ggccgtccgc atccgtgccg acgacgcggg cccggaactc
     2341 cgccaggtac gaatccgtgt agtagagccg ctcggtcact cgactgcgat ggtccgtgaa
     2401 aacatcacac ccattcgagc gcgcccttct tatacgccca gatgtagccg acgatcagga
     2461 tggcgaggaa cacggccact tcggcgacgc cgaaccagcc gagtttgtga taccggacgg
     2521 cccatggaaa cagaaacacc gcctccacgt cgaatatgac gaacaggatc gcgatgatgt
     2581 agaaccgcac cgtataccgg ccgcgcgaat ccgaggcagc cttgatgcca cactcgtatg
     2641 cgtcgagttt gactatgctg ggcaaagccg gcctcaccag cctcgccgcc accaggacga
     2701 cgacggggaa caggatggac agcaccagga agacgaatat cggaatatac ccttctggca
     2761 tggaattaca tcttatcacg cacccggctg caccccagaa gcttccctac cgcaccaccc
     2821 gcgccagcat ttcgacggtt tcttcgatct cttcgcgtgt tgtggtcctt cccaggctga
     2881 atcggatagc gcccattccc acctcgggcg ggatctgcat tgcctgaagc accggcgaca
     2941 gttcgacaca gcccgagtgg caggctgatc cggtcgaagc cgcgacgccg tccaaccgtg
     3001 ccaggatgtc ggccccagcc cgacccgtga agctcacgtt cagcgtgttc ggcagccgtt
     3061 cctccggatg tccgttcagc acgacccctt cgccaaactg attccgcagg aggttccaga
     3121 acagatctcg aagctcccgg agccgcgttc cgccggtcca caaccgcgcg agttcgcagg
     3181 ccgccccgag gccgacatcg agcagcacgt tttccgtgcc cgcgcgccgt cctgattcgt
     3241 ggccggcgcc gtggaccaac ggctcgatgg caaccccatc acgaacgaac agagcgccca
     3301 ctcccttcgg agcatagagc ttgtggcctg ccaccgacaa cagatccacg ccaagatcgt
     3361 ggacgttcgt ggagatcttc cctgccgatt gcgccgcgtc ggtgtggaac agggcgtcac
     3421 gctcgcgcgc gatacgtgcg atggctgcta ccggctggat cgtgccaacc tcgttgttcg
     3481 catgcatcac cgagatcagg atggtcgatg gtctcaacgc ccggcgtacg tcctccgggt
     3541 ccacgcggcc gtaacggtcc actggcagat aggtcaccgc cgcgccgagc cgctccagaa
     3601 accggcatgg gctgatgacc gcggggtgct ccacctgcgt tgtgatgata tgcgcgcccg
     3661 aaccgccctt tgcgaagaac acccctttga tcgcgtggtt gttcgcctcg gtgccgccgc
     3721 tggtgaagac aatttcccgt ggccggcaat caagcaggcc cgctacctgc ccccgagcgt
     3781 tctcaaccgc ttcgcgagcc tgcacgcctg cccaatgagt gctcgatggg ttgccgtaat
     3841 ggccttcgag aaacgggctc atcgcatgcg ccacctcggg agcgataggc gtactggcat
     3901 tgaaatcaag atagatgcgc cgcaacattc cagcccaatc ctcaacccac tcttccgaat
     3961 atgccacatc gggagataat aggaccagga tgttccgaac cattaaggaa caggtggata
     4021 caatcttccg ggaggacccg gccgccaaga gcgtgctcga gatcctgctc tgctaccccg
     4081 gattccacgc tgtcctgctc catcgtctcg ctcacaagtt ctaccgctgg aatattcccg
     4141 tgatcgcccg ggtcatgtct caggtcagcc gcttcttcac cggcatcgaa atccacccgg
     4201 gcgccaggat cggccgccgg ttctttatcg atcacggcat gggcgtcgtg attggcgaaa
     4261 ccaccgaaat cggagacgac gtcctgctct atcagggcgt gacgctcggc ggaaccggca
     4321 aggaaaaagg taaacgtcat ccgacaatcg gaaacaacgt cgttatcggg accggcgcga
     4381 aggtcctcgg caacattcgg attggcgacc acgtgaagat cggagccggc tcggttgtcg
     4441 ttcatccggt gcctgaccac tcgacagttg tcggcgtccc cgggcgtgtc gttcgccgcg
     4501 caaccgaggt tcctgaatcc gaggacgtgc tcgaacacgg ccggctacct gatcctgaag
     4561 cccaggcaat cgacgagctc gcccagcggc tcgaacagct cgaggccgcg gtaagaatgc
     4621 tctctgagga taaggccacc gcgaggcgcg gatcttaagt ccgatgtccc cggaggtgcg
     4681 aaacgcctcg cggcaaccag tgggggcagc acaaaagata acatcggtta tgggagcccc
     4741 actacttttt ggccg
//
LOCUS       DATIFQ010000423         6814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_908201, whole genome shotgun sequence.
ACCESSION   DATIFQ010000423 DATIFQ010000000
VERSION     DATIFQ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908201"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VLE22_21145"
     CDS             <1..393
                     /locus_tag="VLE22_21145"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HSB16971.1"
                     /translation="PWCVRAFSKMPESSMRRDRQRLHDILDALDSLPRIIAGHTEAEF
                     LDNETTRYAVAQRLAVVGEAAGRISTELRERHPAVPWIDIVSFRNILVHEYFGIHWPI
                     VWLTATEQAPYLRKQVARILETEFPNPG"
     gene            complement(410..1534)
                     /locus_tag="VLE22_21150"
     CDS             complement(410..1534)
                     /locus_tag="VLE22_21150"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HSB16972.1"
                     /translation="MRTDRRAFLKMAGLGAATLAAGSPALSAGAIKNWVWIPTNTRSS
                     ADHWKRRFALIRESGVRAILPEVYAGREAYFPSTRLPVKTDWLGMLLPLARAEGLEVH
                     AWMWCMPCMIEEVMKKHPDWYNVNAKGESAVDKPAYVPYYRFLDPGHPEVREFVQATV
                     RELAGIGELAGVHLDYIRHPDAILPKGLWKKYNIVQDRVFPEYDYGYTDYERKRFKEK
                     YGADPLEIKDPKLTEKWFQFRLDMVTDLVNRYLVPAAKTKKKMITAAVFPGPSMARTH
                     VMQDWGRWRLDAFLPMLYHSFYEAGPEWVGDQTREGVSAVKKPLFSGLFVSGMDEAEF
                     TKTVEISSRAGASGVSLFSDDAMDEAKWRALKRITTGGRG"
     gene            1615..2502
                     /gene="xrt"
                     /locus_tag="VLE22_21155"
     CDS             1615..2502
                     /gene="xrt"
                     /locus_tag="VLE22_21155"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HSB16973.1"
                     /translation="MAPTPRTMPRDSASSSARAPVPWLSIAWFGALLAACYAPVLTRL
                     VRNWIDDPDMGHAFFVPVIAGYIAWQRRDEILACPLRSSPWGLAVVAFGALQLYVATL
                     GAELFLSRTAFIISLSGVVLYFGGWRLLRLLAFPLFLLCFMVPIPAILYNQVTFPLQL
                     IASRVAEVSLSLLGLPVLREGNVIELPSQTLNVVEACSGIRSLLSLSFLSLVYAYFFD
                     KKVWMRAALLLATIPIAIAANASRVTLTGILSEYQPDLAEGFFHSAEGWVIFMVALVM
                     LIVVHRLIDWGYRTLRGKG"
     gene            2507..3163
                     /locus_tag="VLE22_21160"
     CDS             2507..3163
                     /locus_tag="VLE22_21160"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HSB16974.1"
                     /translation="MPGFLKSRSARVLTLVLAVQAILSYTLSRGEAVPLAAPLSGFPS
                     QVGEWTLASEGVIEKEIRDVLRADDILNRVYTDADGRQSASLFVAYFKTQRTGQAPHS
                     PKNCLPGSGWVPSESETAVIPISGRAEPIKVNRYVVSRGSNRSVVMYWYQSRDRVIAS
                     EYAAKIRLVLDSIRYNRSDTALIRVVVPVAGDNEAAATATATRFVQTVFEPLSRQLPR
                     "
     gene            3225..4427
                     /locus_tag="VLE22_21165"
     CDS             3225..4427
                     /locus_tag="VLE22_21165"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HSB16975.1"
                     /translation="MTLSTPFRSAVLASAIGLCVAGAWSQPAAPETEKSGEPVVIGER
                     LSILSEILKEPRPLLVAKPAGYESETERYPVLYLLDGDAHFHHVTGIVSFLTESGRIP
                     KLLVVAVPNTNRTRDLTPPSTAEIDLRFSPGGGGADAFLRFLGDELVPYLEKNYRTRP
                     YRILAGHSFGGLFAIHAMITRPKLFNAYIAIDPSLSWNNQAPVSQAEAFFETTKELQS
                     DLYVTATSEGGGIRKLAGVLEAKAPKGFRWKLELMPEETHVSIPHRSIYQGLDTIFDA
                     WHLANPLELYDKGGIDAVHRHFREGGKRLGYDRTTSAFTISMIVAGLMQAGRLEEAAS
                     VLLHDPKAYPPPWNQLDALARAYAKRGDTGQVVRYYTLSLKENPRNEWARKKLAELGV
                     EPATLPPQ"
     gene            4441..6588
                     /locus_tag="VLE22_21170"
     CDS             4441..6588
                     /locus_tag="VLE22_21170"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB16976.1"
                     /translation="MLCLRTLALPIMIASFLPALSQEPRATIRVEVRTDSAPVAGANV
                     KLNAVSVITGPDGIAITSTPFGKVDVTVTKEGYHPSTASLSIDQPREWQLVIDLQPVQ
                     HIEEEITVHATRTDRRLQDLPTRVEVLGREEIEEKMLMTPGDIVMMLNEMGGLRVQTT
                     SPSLGAASVRIQGMRGRYTRFLADGLPLFGQQGGGLGLLQIPPMDLGQVEVIKGASSA
                     LYGAGAMAGVVNLISRRPGPEPVHELLVNRSTLGATDTSLFLASRLSPRWSASLLGGG
                     HWQERNDRDGDGWADLAGYARGVARPRFFWDGGNGRAGFLTGGITYESRKGGTLPGEV
                     LPATGAPYAEALNTRRYDLGGSFQFLVANRHVVTTRLAASSQNHDHRFGEIRERDRHD
                     MLFGEIAARGASRRHTWVVGLAVDRDAYRPRDVSRFDYEYTTPGVFLQEDIDIASWVS
                     LSASARADFHSKYGTFFSPRLSALFRWEGWTSRLSAGQGFFAPTPLTEETEAAGLTRL
                     TIPVPLVAERGRTASLDVTRALGPASFTTTVFASTVRHPIDVERAERYELTNLPEPTR
                     NLGLELLGTWRKAPFAATASYTYVRSREWDLGRRADTPLTPRHSFGVVGMWEKEGAAR
                     VGVECYYTGLQRLEENPYRSESEPYVILGFLAERKVGPVRFFLNAENLTNVRQTRWNP
                     LLRPNRGTDGRWTVDAWAPLDGRAFNGGIRLGF"
BASE COUNT         1173 a         2319 c         1990 g         1332 t
ORIGIN      
        1 ccctggtgcg tacgggcgtt ctcaaagatg cccgagtcct ctatgcggcg tgaccgtcaa
       61 cggctgcatg acattctgga cgcgctggat tcgctaccca ggatcatcgc tgggcatacg
      121 gaagccgagt ttctcgacaa tgagacgact cgttatgccg tcgctcagcg gctagccgtc
      181 gtcggcgaag cggccggcag aatcagtact gaactccgtg agcgccatcc ggcggtccca
      241 tggattgaca tcgtctcctt ccgaaacatt ctggtccatg agtactttgg gattcactgg
      301 cccatcgttt ggctcacggc aaccgaacaa gctccctacc tgaggaagca agtcgcacga
      361 atacttgaga ctgagttccc caaccccggc taaaaacgcg taaagctctc tacccccgtc
      421 cccctgtcgt aatccttttc agcgcccgcc acttcgcttc atccatcgcg tcgtcggaga
      481 agagggacac gccggatgct ccggcccgcg aggagatttc caccgttttt gtgaactcgg
      541 cctcgtccat cccgctcacg aacagcccgc tgaacagggg cttctttacc gcggacaccc
      601 cttcccgcgt ctgatcgccg acccactccg gaccggcttc atagaacgag tggtacagca
      661 tcggcaggaa tgcgtccagg cgccaccggc cccaatcctg catgacgtgc gttcgcgcca
      721 tgctcggccc gggaaacacg gccgccgtga tcatcttctt cttcgttttc gcggccggaa
      781 ccagatagcg gttcaccaag tccgtcacca tgtccagacg gaactggaac cacttctccg
      841 tcagcttggg gtctttgatc tccagcggat cggccccgta cttctccttg aaccgcttcc
      901 gctcgtaatc cgtgtagccg taatcgtact caggaaagac cctgtcctgt acgatgttgt
      961 atttcttcca gagccctttc gggagaatcg cgtccgggtg gcggatgtag tccagatgaa
     1021 cgcccgccag ttccccgatg cctgccagtt ccctcaccgt ggcctgcacg aactcccgaa
     1081 cctcggggtg ccccgggtcc agaaaccggt aatacggcac atatgcgggc ttgtccaccg
     1141 ccgactcgcc ttttgcgttc acgttgtacc agtccggatg cttcttcatg acttcttcga
     1201 tcatgcaggg catgcaccac atccacgcgt gcacttcgag gccttccgcc cgcgccagcg
     1261 gcagcagcat cccgagccag tccgttttca caggcagccg tgtactcgga aaatacgcct
     1321 cgcggcccgc gtagacctcc ggcagaatcg cccgcactcc cgactcccga atcagcgcga
     1381 agcggcgctt ccagtggtcc gctgaactcc tggtgtttgt gggaatccaa acccaattct
     1441 tgattgcgcc ggcggataaa gctggtgaac cagcggcaag ggtggccgct ccgaggcccg
     1501 ccatcttcag aaacgcgcgt ctgtcagtcc gcatagggtc catcctactc ccggtattgg
     1561 aacctgagaa gagccgtgga tgccgtagct gtgctctact gtatggagtt acggatggcc
     1621 cccactcctc gcacgatgcc ccgggacagt gcctcatcga gcgcgcgcgc ccctgtcccc
     1681 tggctctcga tcgcctggtt cggagctttg ctcgccgcct gttacgcgcc cgtcctcacg
     1741 cggctcgtcc gcaattggat cgacgacccg gacatggggc acgctttctt cgtccccgtg
     1801 atcgccggtt acatcgcatg gcagcggcgg gacgagatac tcgcctgccc gttgcgctcg
     1861 agcccctggg ggctggcggt ggtggccttc ggcgctcttc aactctacgt cgccaccctc
     1921 ggcgccgagc tgtttctgtc caggaccgcc ttcatcatct ccctctccgg cgtcgtcctg
     1981 tactttggcg gctggaggct gttgcggctc ctcgccttcc cgctgtttct gctttgcttc
     2041 atggtgccca tcccggcgat cctctataat caggtcacct tcccgctcca gttgatcgcc
     2101 agccgcgtgg ccgaggtttc gttgtcgctg ctgggtctgc ccgttctgcg cgaaggaaac
     2161 gtgatcgagc tgcccagcca gacgctgaac gtcgtcgaag cgtgcagcgg cattcgctct
     2221 ctcctctcgc tctcgttcct atcgctggtt tacgcctact tctttgacaa aaaggtctgg
     2281 atgcgcgcgg ctctcttgct cgccaccatc cccatcgcga tcgccgccaa cgcgagccgc
     2341 gtcaccctca ccggcatcct gagcgaatac cagcccgacc ttgcggaagg tttcttccat
     2401 tccgcggagg gatgggtgat ttttatggtc gcgctggtga tgctgatcgt agtccaccgg
     2461 ctgatcgact ggggttaccg gactttgcgt gggaagggat agcggaatgc cgggattcct
     2521 caaaagcagg tcagcgcgag ttctcacctt ggtgctcgcg gtgcaggcga ttctctctta
     2581 cacgctctcg cgcggggagg cggttcccct ggcggccccc ctgagcgggt ttccgtccca
     2641 ggtcggcgag tggacgctcg ccagcgaagg cgtgattgaa aaggaaatcc gcgacgtctt
     2701 gcgcgcggat gacatcctga atcgcgtcta cacggatgcg gacgggcgtc agtctgccag
     2761 cctgttcgtc gcgtacttca agacgcagcg caccggccag gccccgcact cgccgaagaa
     2821 ctgcctgccg ggctcgggct gggtgccttc ggagtccgag acggccgtaa tccccataag
     2881 cggccgcgcc gaacccatta aggtcaaccg ctacgtggtc tcccgcggga gtaacagaag
     2941 cgtcgtgatg tattggtacc agagccgcga ccgggtaatc gccagtgagt acgctgccaa
     3001 gatccgcttg gtgctcgatt cgatccgcta caaccgctcc gacacggccc tcattcgcgt
     3061 cgtcgtccca gtcgccggcg ataacgaagc cgccgccacc gcgaccgcca cccgcttcgt
     3121 ccagaccgtc ttcgaacccc tcagccggca gttgccgcgg tagcatcgcg tctcgaccct
     3181 cgcacactcg gtctccctgt agactggttc gggaggtggt ccgggtgaca ttgtcgacac
     3241 cgttccgttc ggcggtcctt gcctccgcca tcggcttgtg tgtcgccggt gcctggagtc
     3301 agccggccgc acccgagacg gagaagagcg gcgagccggt cgtcatcggt gagaggcttt
     3361 cgattctgtc tgaaatcctg aaggagccgc ggccgctcct cgtcgcgaaa ccggccggct
     3421 acgaatcgga gaccgaacgt tacccggtcc tctatcttct ggacggcgac gcgcacttcc
     3481 atcacgtaac cggaatcgtc agtttcctga ccgagagcgg gcgtatcccc aagttgctgg
     3541 tggtggccgt gcccaacacg aaccgcaccc gggacctgac gcctccctcg acagcggaaa
     3601 tcgaccttcg attctctcct ggcggcgggg gagccgatgc cttccttcgc ttcctcggtg
     3661 acgaactggt gccgtacctg gagaagaact accggacgcg gccctacagg atactggcag
     3721 gccactcctt cggcggtctc ttcgcgattc atgcgatgat caccaggccg aagctgttca
     3781 acgcctacat tgccatcgat ccgagcctct cgtggaacaa ccaggcgccg gtgtcccagg
     3841 ccgaagcgtt cttcgagacc acgaaggagc tgcaaagcga cctctacgtg accgccacca
     3901 gcgagggcgg cggcattcgc aaactggccg gagtacttga ggcaaaggcg cccaagggat
     3961 ttcgctggaa acttgaattg atgccggagg agactcacgt ctcgattcct catcgcagca
     4021 tctaccaggg tctcgatacg atcttcgatg cctggcactt ggcgaaccct ctggagctat
     4081 acgacaaagg cggcatcgac gccgtccacc ggcactttcg ggagggcggc aaaaggcttg
     4141 gctacgaccg gacgacttcc gcgttcacaa tttccatgat cgtcgccggg cttatgcaag
     4201 cgggccggct ggaggaggcg gcttcggtcc tcctgcatga tcccaaggcg tatccgccgc
     4261 cctggaacca gttggatgcg cttgcccgcg cctacgcgaa gcgcggcgac accgggcagg
     4321 tcgtccgtta ctacacgctg tcgctgaaag agaaccctcg caacgaatgg gcccggaaga
     4381 aactcgcgga actcggcgtc gaacccgcca cactaccgcc gcagtaaccc agccacccac
     4441 atgctctgcc ttaggaccct cgccctgccc atcatgatcg cctctttcct gccggctctc
     4501 tcgcaggagc cgcgcgcgac gattcgggtg gaagtgcgga cggattccgc tcccgtagca
     4561 ggcgcgaatg tgaagctgaa cgcggtctcc gtgatcaccg gcccagacgg tatcgccatt
     4621 acttccactc ctttcgggaa agtggacgtc accgtcacca aggagggcta ccatccctcc
     4681 actgcatctc tgtcaatcga ccagccgcgc gagtggcagc tcgtgatcga tcttcagccg
     4741 gtgcagcaca tcgaagaaga gataaccgtg cacgcgacga gaaccgaccg gcgcctccag
     4801 gacctgccca cccgagtcga ggtgctgggc cgggaagaga tcgaggagaa gatgctgatg
     4861 acccccggcg acatcgtcat gatgttgaac gaaatgggtg gtctccgcgt gcagacgacg
     4921 tcgccctcgc tcggcgcggc tagcgtgcgg attcagggca tgcgcggccg ctacacacgc
     4981 tttctcgccg acggactgcc cttatttggc cagcagggcg gcgggctcgg tcttttgcag
     5041 attcccccta tggacctcgg ccaggtcgag gtgatcaagg gcgcctcgtc cgcgctctac
     5101 ggcgccggag ccatggccgg cgtcgtaaac ctcatctccc gccgccccgg cccggagcct
     5161 gtccacgagc ttctcgtgaa tcgctccacc ttgggcgcga ccgacacgtc gctctttctg
     5221 gcgtcgcggc tgtctccgcg ctggagtgcc tccctgctcg gcggcggtca ctggcaggaa
     5281 cggaatgatc gagatggcga cggctgggcc gacctcgcgg gatacgcgcg cggcgtcgcc
     5341 aggccgcgct tcttctggga cggcggcaac ggccgggccg gcttcctcac cggcgggatc
     5401 acgtatgaga gccggaaggg cggcacattg cccggagagg tcctcccggc gaccggcgcc
     5461 ccttacgcgg aagcgttgaa cacccgccgg tacgacctcg gcggcagctt ccagttcctt
     5521 gtcgcaaatc gccacgtcgt taccacccgt ctcgccgctt cctcgcaaaa ccacgatcac
     5581 cgtttcggcg agatccggga acgcgaccgc cacgacatgc tgttcgggga gatcgcagcc
     5641 cgaggcgcct ccagacgcca cacttgggtg gttggcctgg ccgtcgatcg tgacgcctac
     5701 cgtccgaggg acgtttcgcg cttcgactac gaatacacca cccccggcgt gttccttcag
     5761 gaggacatcg acattgcctc ttgggtctcc ctttcggcca gcgcgcgcgc cgacttccac
     5821 agcaagtacg gcacattttt cagcccccga ctttcggccc tgtttcgatg ggaaggctgg
     5881 accagccgcc tttccgccgg acagggcttt ttcgccccca cgcctttgac cgaggaaacc
     5941 gaagccgcgg gcctgactcg cctgaccatt ccagtgccgc tcgtggcgga gcgcggccga
     6001 accgcttcgc tcgacgtcac cagagccctc ggtccggcct ctttcacaac gacggtcttc
     6061 gcctccaccg tgcgtcaccc catcgacgtc gagcgcgctg aacgctatga actcaccaat
     6121 ctgcccgagc cgacgaggaa tctcggcctg gaactgcttg gcacctggag aaaggctccg
     6181 ttcgcggcca cggcgagcta cacctatgtg cgctcccgcg agtgggacct gggccggcgc
     6241 gctgatacgc cgctgactcc gcgccatagc ttcggagtcg tcggcatgtg ggagaaggaa
     6301 ggagccgcgc gcgttggagt cgaatgctac tacacaggcc tccaacgtct ggaggagaac
     6361 ccataccgct ccgagtccga gccttacgtg atcctgggat tcctggccga acgcaaggtt
     6421 ggtcccgtgc ggttctttct gaacgcggag aacctcacca atgttcgcca gacccgttgg
     6481 aacccgttgc tccggccgaa tcgcggcacg gacggccgtt ggacggtcga cgcctgggcg
     6541 cccctggacg gccgcgcctt caacggcggt atccgcctcg gcttctgacc ccgctcgata
     6601 ccgaacgaat gctcgcgact cttcgcccgt aattggggag tgcgccatga gccgaccacg
     6661 gtgcgcggcg atcaccgggc atcgggtcac caacctcctg ctccagtgct ttgagccgat
     6721 aaacacattg ccgcgccgcg gcgacttgtg gtacacccaa gctcaggtcg ttttgtcgtc
     6781 tagtgacagc tcctcgaccg aagttcgcga tgtg
//
LOCUS       DATIFQ010000424         8923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1482135, whole genome shotgun sequence.
ACCESSION   DATIFQ010000424 DATIFQ010000000
VERSION     DATIFQ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8923
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482135"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VLE22_21175"
     CDS             <1..1026
                     /locus_tag="VLE22_21175"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB16977.1"
                     /translation="GAWFATSGAQPSVPPVSPIPTEEGQLVVFDKSTWQMRELTKRIP
                     LNDSWPQGQEMERALERFGLTRITPAEHIPVPAQIAPDVYLIGQDRVSNLTYMIDCGP
                     EGVAVIDPTYASEAERTMANVEKVGRPRKDIRWVVNTHCHFDHAMADKQFRDAGAEIM
                     VHAIDADHIEKGSQVTGWGMFAKQTGGGEFPRCKIDRRLTDGEELTLGNKKLLVIHTP
                     GHTPGSASFLLETGGKKLLFSGDTVLYDCRLGWQENPYADNRQYLVSFEKLDHFIFNG
                     QRMLWDVLLPGHGAISMDRAYLDVKKGRELVAASLAAGEPILSVPYTTEYYRARMYGR
                     PATPAQR"
     gene            1040..2155
                     /locus_tag="VLE22_21180"
     CDS             1040..2155
                     /locus_tag="VLE22_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16978.1"
                     /translation="MRSAAILLCLSVPLFSSNRRISILWEKAGAQGEVQVSRGTLASG
                     KPRFDCATQTPCRIDVTVGDSGVALGEAATLVTVSATPNSFTFFLRDVSNEWPIFIPE
                     YGVAVTTGDDRRSYAEIEHEIRGRGLQSKLEQVESQPEESFDDAARNVRNLQVQTWLG
                     LGRDIRIFAVGERLDWIQPRFHGYEVKLPEAEDKPVRYSFLTGRGWGPRDQITRRLEE
                     GVLPILQARLVDENVTYEITAFTTMETSPVREGSVRGTHFLVADGFGHGHMFTPEQQK
                     LHDSLLPAETNQPEETVLYLRAVAVNTSRAPAYAFFKHVSPSNVPSSEWKFDGTRGYG
                     VYKSGRVFSVATLDAKPLPREEAALLLAPGGSAVLDA"
     gene            2148..3668
                     /locus_tag="VLE22_21185"
     CDS             2148..3668
                     /locus_tag="VLE22_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16979.1"
                     /translation="MLEIRLPHRPISAERAAKLAVQDFAARHVEARDFWHCKLRSAAR
                     VHLPEKRIEEMIQAGLLHLDLVTYGLEPEGTLTSTIGVYSAIGSESSPIIQFMDAMGR
                     HDVAQRALQYFLDKQHDDGFIQNFGGYMLETGAALWSIGEHYRYTRDDNWARKVEPKV
                     VKACRYMQEWRKRNMREDFRGRGYGMLDGKTADPEDPFHSFMLNGYAYLGFARSAEML
                     ERTDPAESRRWREEADGLRRDIRESFRAAMGRSPLVPLRDGTWCPTAPPWAEYRGPLA
                     LGTDGGSWFTHGAMVSRDSLLGPIYLIFQEVLEPDEPASSFLLRFHNELMTDRNVAFS
                     QPYYSRHPVSHLRRGEVKPFLKAYYNTVAALADRQTYTFWEHYFGASPHKTHEEGWFL
                     MDTRWMLYMERGDTLSLLPGAPRRWLEDGQRIELRNVASYFGPLSFEVVSDIAHGRIE
                     AVIECDPERKPKTVEIRLPHPLGRKAVRAEGGTYDPATERVRIENFTGRTNVGLRF"
     gene            3754..4974
                     /locus_tag="VLE22_21190"
     CDS             3754..4974
                     /locus_tag="VLE22_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16980.1"
                     /translation="MRCTAFALVALWSAPLLVRAQDNAELLSRIKAMEERIRALESEV
                     QVLKSQQQPAPSPPPEPTQPPAPSPPPEPVQAFPQAAAGASKVFNPDIAVIGNFFGAA
                     GQDAGRGAPALEMHESELSLQAVVDPYARADFFLSFGEQGVNLEEGYLTFTALPAGLQ
                     LRAGKMRGAFGRVNTFHTHVLPWVDRPLVTQNLLGGEDGLNDAGLSLSRILPAPKGVF
                     LEATAQVFRGDSEGVFQSWHRSNLSAIGHLRGYRDISESTNLDLGVSYARGHSPEGPS
                     LINQLYGLDATLRWKPLRRSIYRSMVTRSEFVWGRAQLPSEVARPFGYYVSSDYQFAR
                     RWFFGGRLDRSERFSNTTLPDFGGSLVLTFWPSEFSQVRGQLRRTRYDDRQIANELLF
                     QFQFSIGAHGAHPF"
     gene            5041..5958
                     /locus_tag="VLE22_21195"
     CDS             5041..5958
                     /locus_tag="VLE22_21195"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HSB16981.1"
                     /translation="MIHPFRLLYVLLLAGLTASAASKPNVVTATEDLAALAREVGGDR
                     VTVEAIARGYQDPHFVEPKPSFILKLQKADLLVAVGLQLEIGWLPPLINQSRNSKIQP
                     GGSGYLDVSAACKILDIPTGPVTRAMGDVHPLGNPHYWLDPENGSRIARAIAEKLAQL
                     DPSNTAYYGQRRADFERRLTEAQKRWLARLAPYRGSKVITYHRSWPNFAAYFGLEVVD
                     YVEPKPGIPPTPSHTLDLINTMNREKIKLILVEPYFDLRTPNSIAEKTGSQVVVLMPS
                     VGGVKQVQDYFQLFDYDIELLVKAFEKVK"
     gene            5967..6794
                     /locus_tag="VLE22_21200"
     CDS             5967..6794
                     /locus_tag="VLE22_21200"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HSB16982.1"
                     /translation="MDALSFLAAPFIASLILTGIHAYLGVHVVERGVIFVDLSLAQIA
                     ALGTTIAVLAGIEPHGDGAYVVSLAFTFVGAAVFSTIRGHKARIPQEAVIGITYAVAS
                     AAAILAMSKSMEQTEHLKEMLVGNILTVSWLEVGKTALLYGAVGLFHYIFRRRFLSIT
                     LDQRAAEAQGVSVRFWDFLFYASFGFVVTSSVAIAGVLLVFCYLIVPSVGAMLYAESI
                     GPRLAIGWTMGTLVSALGVYLSLKLDLPTGATIVCTFGIVLVLMAALRTFAPRRSRA"
     gene            complement(6882..7469)
                     /locus_tag="VLE22_21205"
     CDS             complement(6882..7469)
                     /locus_tag="VLE22_21205"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSB16983.1"
                     /translation="MGRRSMDDDSNPHDARTGLESLRDVLKAALFAAEKHANQRRRGA
                     RAQPYIQHPLEVALMVAQALPERDTNLIQAALLHDCLEDAEVTYSQLEEEFGADVAAL
                     VLEVTDDKSLPKQTRKNLQVESAPTKSVRAQYIRVADKISNLRSIMNDPPIDWTPERQ
                     REYVRWAKAVIDRLTSPPPSLKEEFDWTYRRLLGD"
     gene            7560..8783
                     /locus_tag="VLE22_21210"
     CDS             7560..8783
                     /locus_tag="VLE22_21210"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB16984.1"
                     /translation="MTRRAWIKLAAWVPAGAAFSRFNLLAQPNRGKVKITGIRAMALK
                     NIAGNCLIRIDTDAGLVGYGEAGSTGPMARARIETMRGLLVGKDPLAVEVHFHHMTTL
                     MHTYMAHIPTISGIDIALWDLAGKILGRPVCELLGGPFREAIPMYSHGIGLDMLEKTS
                     CRDWAQRIRQQPEGFSAYKIAIDPVLGVPPARFANTLDGAQLRKVARAYANVREAVGD
                     EIDIAVHCHNEFDTPSAIGVAKAVEPVNPLFYEDALNVPFSEGWMALKRSTRIPILTG
                     EKLELVRGFRPFLDTQAADIVHPDLAFAGGITGTKKIADYAALTRTPVALHNVGSLVL
                     CYANAHFGASIQNFYRSESALGRPNRYVESMAASNPPEVHNGQLKVPEGPGLGLDLNE
                     DVLRSNLAPGEEYWS"
BASE COUNT         1640 a         2679 c         2908 g         1696 t
ORIGIN      
        1 ggcgcttggt ttgcgacctc cggcgctcaa cccagcgtgc ctcccgttag cccaattccg
       61 accgaagaag ggcaactcgt cgtgttcgat aagagcacct ggcaaatgcg cgagttgacc
      121 aagcgcattc ctctcaacga ttcatggccg caagggcagg agatggagcg tgcgctcgag
      181 cgcttcggtc tcacccgaat cacccccgcg gagcacatcc cggtaccggc gcagatcgca
      241 ccggacgtgt atctgatcgg gcaggaccgc gtcagcaatc tgacttatat gatcgactgc
      301 ggacccgagg gcgtcgccgt aatcgacccc acatacgcct cagaggccga gcgcaccatg
      361 gccaacgtcg aaaaggtagg acgtccgcgg aaggacatcc gttgggtagt caacacgcac
      421 tgccatttcg accacgccat ggccgacaag cagttccgcg acgccggcgc cgagatcatg
      481 gtgcacgcca tcgacgcgga tcatatcgaa aagggctcgc aggtgaccgg ctggggaatg
      541 tttgccaaac agaccggcgg cggagagttc ccgcgctgca agatcgaccg gcgcctcacg
      601 gacggcgagg aactgacgct cggcaacaag aagctgctgg tcattcacac gcccgggcac
      661 acgccggggt cggcgtcgtt cctgctcgaa actggcggca agaagctcct gttctcggga
      721 gacacggtgc tttacgactg ccggctcgga tggcaggaga atccctacgc cgacaaccgg
      781 cagtatctcg tgtcgttcga aaagctcgat catttcatct tcaacgggca gcggatgctt
      841 tgggatgtgt tgctgccggg ccatggcgcc atttcgatgg atagggccta cctcgatgtg
      901 aagaagggac gcgaactcgt ggccgcgagc ctcgccgccg gcgagccgat cctttcggtg
      961 ccttacacga ccgagtatta ccgtgcgcgg atgtacggac gccccgcaac gccggctcag
     1021 cgctaaggag cacgaaaagg tgcgatccgc ggccattctg ctgtgccttt ccgtgcctct
     1081 tttctcttca aatcggcgca tctcgattct gtgggagaag gccggtgccc aaggtgaggt
     1141 ccaggtgagc cgcggaactc tcgcgagtgg gaagccgcgc tttgactgcg cgacgcaaac
     1201 tccctgtcgc atcgatgtca ccgtcgggga ttccggtgtc gcgctgggcg aagccgcgac
     1261 actggtcacg gtatcggcga cgcccaattc gttcaccttt ttcctgcggg acgtctcgaa
     1321 cgagtggccc atcttcatcc cagagtacgg agtcgccgtc accacgggcg acgaccggcg
     1381 ctcgtacgcg gagatcgaac acgaaatccg cgggcggggt ctccaaagca aactggagca
     1441 ggttgagagc cagccggagg aatcgttcga cgacgccgcg cgcaacgtcc gcaatcttca
     1501 ggtgcagacc tggctcggat tgggcaggga cattcgcatc ttcgccgtcg gcgagcggct
     1561 cgactggatc cagccgcgtt ttcacggcta cgaggtgaag cttccggagg cggaagacaa
     1621 gcccgttcgc tacagtttcc tcaccgggcg aggatggggc ccgcgggatc agatcacgcg
     1681 ccggctagaa gagggagtac tgccgatcct gcaggcgcgg ctcgtcgacg aaaacgtcac
     1741 gtacgagata acggcgttca ccacgatgga gaccagcccg gtgcgggaag gctccgtccg
     1801 cggcacgcat ttcctggtcg ccgacggctt cggacacggc cacatgttca cgcccgaaca
     1861 acagaagctc cacgattcgc tgcttcctgc cgagacgaac cagccggagg agaccgtcct
     1921 ctacctgcgc gctgtggcgg tgaacacatc acgtgctccc gcatacgcgt tcttcaagca
     1981 tgtttcgccg tcgaacgtgc cgtcgtcaga gtggaagttt gacggcaccc gcggctacgg
     2041 cgtgtataaa agcgggcggg tgttcagcgt ggccacgctc gatgcgaagc ctctgccgcg
     2101 cgaggaagcg gcgttgttgc tcgcgccggg cgggtcggcg gtgcttgatg cttgagatcc
     2161 gattgccgca ccggccaatc tcagccgagc gcgcagcgaa gcttgcagtg caggatttcg
     2221 ccgcacggca tgtcgaggcg cgtgacttct ggcactgcaa gctccggagc gccgcgaggg
     2281 tccatctgcc ggagaagcga atcgaggaga tgatccaagc ggggctgctg cacctcgacc
     2341 tggtgaccta cgggctcgag ccggagggca ctctcacctc gaccatcggc gtttacagcg
     2401 cgatcggttc ggaaagctcg ccgatcatcc agttcatgga cgcgatgggc cggcacgatg
     2461 tggcgcagcg cgcgctacag tactttctcg acaagcagca cgacgacggc ttcattcaga
     2521 atttcggcgg ctacatgctg gagaccggcg cggccttgtg gagtatcggc gagcactacc
     2581 ggtacacccg ggatgacaat tgggcgcgca aagtggagcc gaaggtcgtc aaggcgtgcc
     2641 ggtacatgca ggagtggcgc aaacgcaaca tgcgggagga ctttcgcggg cgcggctacg
     2701 ggatgctgga cggcaagacc gccgacccgg aggacccgtt ccactcgttc atgctgaacg
     2761 gttacgccta cctcgggttc gcccgctcgg cggaaatgct ggagcggacc gacccggccg
     2821 aatctcggcg ctggcgggag gaggccgatg gccttaggcg ggacatacgc gagtcgttcc
     2881 gcgcggcgat gggccgctcg ccgctggtcc cgttacggga cgggacatgg tgcccgactg
     2941 caccgccgtg ggccgagtac aggggaccgt tggcgctcgg tacagacggc ggttcgtggt
     3001 tcacgcacgg ggcgatggtc agccgcgact cgttgctcgg tccgatctac ctgattttcc
     3061 aggaagtgct ggaaccggac gagccggcct cgtcgtttct gctgcgcttc cacaacgagc
     3121 tgatgacgga ccgtaacgtc gccttcagcc agccctacta cagccggcat cccgtatctc
     3181 acctgaggcg cggcgaggtg aaaccgttcc tgaaggcgta ctacaacacg gtggcggctc
     3241 tggccgaccg gcagacctac accttctggg agcattactt cggcgccagc ccccacaaga
     3301 cgcacgagga gggctggttc ctcatggaca cgcgctggat gctttacatg gaacgcggcg
     3361 acacgctctc gctgttgccc ggcgcaccgc gccgctggct tgaggacggg cagcgaatcg
     3421 agctgcgaaa cgtcgcgagt tacttcgggc cgctgtcgtt tgaggttgtc tcggacatcg
     3481 cgcacgggcg gatcgaggct gtcatcgaat gcgatccgga gcgcaagccg aagacggtgg
     3541 agatccgcct gccgcatccg ctcggccgca aggcagtacg tgccgagggc ggaacctacg
     3601 acccggccac cgagcgcgtt cgcattgaga actttaccgg cagaacgaat gttgggctga
     3661 ggttctagcg ggccgtcgga acacgccgac cggaagctag agccataatg ggaatttggg
     3721 gttcccacac cacacctgga gaaaggtgac actatgcggt gtaccgcgtt cgcactcgtc
     3781 gcactgtggt cggcgccgct gttggttcga gctcaggaca atgcggagct gttgagccgg
     3841 attaaagcga tggaggaacg tatccgcgcg ctggagtccg aagttcaagt tctgaagtcc
     3901 cagcagcagc cggcgccctc cccacctccc gaaccgacgc agccgccggc cccctccccg
     3961 cctcccgaac cggtgcaggc gttcccgcag gcggcggccg gagcgtccaa ggtcttcaac
     4021 cccgacattg ccgtcatcgg caacttcttt ggcgccgccg ggcaggacgc cgggcggggg
     4081 gcgccggctc tcgagatgca tgagagcgag ctgagtctac aggctgtcgt cgacccctac
     4141 gcgcgggcag acttctttct cagcttcggt gagcaggggg tgaacctaga ggagggctat
     4201 ctcacgttta ccgcattgcc cgcggggctt cagcttcggg cgggcaagat gcgcggcgcg
     4261 ttcggacggg tgaatacctt ccatactcac gtcctcccgt gggtggaccg gcccctggtc
     4321 acgcagaacc tgctgggggg agaggacggc ctcaacgacg cggggctgtc gctgagccgc
     4381 atcctgcccg cacccaaggg cgtctttctg gaagcgacgg cccaggtttt tcgcggtgac
     4441 tcggaagggg ttttccagtc ctggcacaga agtaacctgt ctgccattgg ccatctgcgc
     4501 ggttaccggg atatcagcga gtccacaaac ctggacctgg gcgtttccta tgcgcgcggc
     4561 cacagccccg aaggccccag tctgatcaac cagctttatg ggctggatgc cacgctacgc
     4621 tggaagccgc tgcggcggtc gatttaccgt tccatggtca cgcgcagcga gtttgtgtgg
     4681 gggcgggcac aactgccttc cgaagtggcc agaccgttcg gttactatgt gagctcggat
     4741 taccagttcg cgcgacgatg gttcttcgga ggccgtctcg accgttctga gcggttcagc
     4801 aacaccacgc tgccggattt cggcggctca ctggtactga ccttctggcc aagcgaattc
     4861 agccaggttc gagggcaact ccggcgcacg cgctatgacg atcgccagat cgccaacgaa
     4921 cttttgttcc agtttcaatt ctccatcggg gcccacggag cccatccgtt ttgagccgtg
     4981 ggatatcata cgaaacgcgc gcgacgggtt tcaccgtcaa cataaagagg ggagtgccaa
     5041 ttgattcacc cgttccgact cctgtacgtg ctgttgctgg cgggcctgac ggcttccgcc
     5101 gcgtcgaaac ccaacgtcgt gaccgccacc gaggacctcg ctgcccttgc cagggaagtt
     5161 ggcggcgatc gagtcaccgt cgaggccatt gcacgcggtt atcaggaccc ccatttcgtc
     5221 gagccgaagc cgagttttat ccttaagctg caaaaggctg acctactagt agcggtcggc
     5281 ctgcaactgg agatcggttg gcttccgccg ctgatcaacc agagccgcaa ctctaagatt
     5341 cagccggggg gctccggcta tctggacgtc tccgcagcct gcaagatcct ggatatcccc
     5401 accgggccgg tgaccagggc catgggagat gtccatcctc tgggcaatcc gcattattgg
     5461 cttgatcccg agaatgggtc gcgcatcgcc cgggcgattg cggagaaact tgcgcaactg
     5521 gacccttcca ataccgctta ctacggccag cgccgggcgg atttcgaacg ccggctcacg
     5581 gaggctcaga agcgctggct ggcccggttg gcgccgtacc gagggagcaa ggtgattacg
     5641 tatcaccgat cgtggccgaa tttcgcggcc tacttcggtc tggaggtggt ggattatgtc
     5701 gagccgaagc cgggtatccc tcctacgccg agtcacaccc tggatctcat caacacgatg
     5761 aatcgcgaga agatcaagct gatcctcgtc gaaccgtact ttgatctgcg cacgcccaac
     5821 tccatcgcgg aaaagaccgg cagccaggtt gtggtgctga tgccctccgt gggcggggtg
     5881 aaacaggtgc aggattattt ccagttgttt gactacgaca tcgaactgtt ggtgaaggca
     5941 ttcgagaaag tgaaatagga aatccaatgg atgcactctc gttcctagcg gctccgttta
     6001 tcgcgagctt gatcctgacc ggcattcacg cgtacctcgg cgtccatgta gtggagcgcg
     6061 gagtcatctt cgtggacctt tcgttggccc agatcgcggc ccttggcact accatcgctg
     6121 ttttggccgg gatcgaacca catggcgacg gcgcctacgt cgtaagcttg gccttcacct
     6181 ttgtgggggc cgcagtgttc tcgacaattc gcggtcacaa ggcgcggatt ccccaggaag
     6241 cggtgatcgg cattacttac gccgtggcgt ccgctgcggc gattctggct atgagcaagt
     6301 cgatggagca gactgagcat ctgaaggaga tgctcgtagg gaacatcctg acggttagct
     6361 ggctggaagt tggcaagacc gccttgctgt acggggcagt cgggttgttc cattacatct
     6421 ttcgacgcag gtttctttcg atcacgttgg accagcgggc agcggaggcc cagggagtgt
     6481 cggtccgctt ctgggatttc ctgttctatg cctcgttcgg gttcgtggtt acttcgtcgg
     6541 tggccatcgc gggggtgttg ctcgtgttct gctacctgat cgtgccctcg gtgggcgcca
     6601 tgctgtacgc ggagagtatc ggtccgaggc tggcgatcgg atggaccatg ggcacgctgg
     6661 tctcggcact gggcgtctac ctgtccctca aactcgacct gcccacggga gccaccatcg
     6721 tctgcacctt cgggatcgtt cttgtgctga tggcggcgct gcgaacattt gcgccacgca
     6781 ggagccgagc gtaggagctt tacaggtttg gcacttgcga atctggccgc cacttgtgaa
     6841 cgttgcaggt cactgagagt ccgaggagcg gttcccttgt atcagtcacc caacaggcgg
     6901 cggtaggtcc agtcgaactc ctccttcagt gaggggggag gcgaagtcag gcggtcgatg
     6961 acggccttcg cccagcggac gtattcgcgc tggcgctcgg gagtccagtc gataggcggg
     7021 tcattcataa tgctgcggag attcgagatc ttgtccgcga cgcggatgta ctgcgcgcgg
     7081 acggactttg ttggagcgct ctcgacctgt aagttctttc gggtctgctt ggggagcgat
     7141 ttgtcgtcgg tcacttcaag gaccaatgcg gcgacgtccg caccgaattc ctcttccagt
     7201 tgcgagtagg tcacttcggc gtcttcgagg cagtcgtgca ggagcgccgc ctggatgagg
     7261 ttcgtgtcgc gttcgggaag cgcttgggcg accatgagcg ccacttcgag cggatgctgg
     7321 atgtacggtt gcgcgcgggc gcctctgcgg cgctggttgg cgtgcttctc cgcggcgaag
     7381 agcgctgctt tcaggacatc gcgaagagat tcaaggccgg tgcgggcgtc gtgtgggttc
     7441 gagtcgtcgt ccatactccg tcggcccatt gtcacaagtc ccccggcggt acggcaaccg
     7501 acgcttgtgt ttgtgtacac ttgcggctga ccggagcgaa ggagaggagc atgcgacaga
     7561 tgacgcgacg agcttggatc aaactggcgg cgtgggtgcc ggcgggagcg gcgttttccc
     7621 gtttcaacct gctggcgcag ccgaaccgag gcaaggtgaa aatcacaggc atccgggcca
     7681 tggcccttaa gaacatcgcc gggaattgcc tgatccgcat cgacacggac gcgggcttgg
     7741 tcggctacgg cgaggcgggc tcgaccgggc cgatggcgcg ggcgcggatc gagacgatga
     7801 ggggcttgct cgtcggaaag gacccgctgg ccgtcgaggt ccacttccac cacatgacga
     7861 cgctgatgca cacatacatg gcgcacatcc cgaccattag cggcatcgac atcgcgctgt
     7921 gggacctggc ggggaagatc cttggccgtc cggtgtgcga gttactcggc gggccgtttc
     7981 gcgaggcgat cccgatgtac tcgcacggaa tcgggctgga catgctggag aagacgtcct
     8041 gtcgcgactg ggcgcagagg atccggcagc agcccgaagg cttttccgca tacaagatcg
     8101 cgatcgaccc cgtgctgggc gtaccgccgg cgcgattcgc gaacacgctc gacggggcgc
     8161 agctccggaa ggtagcacgg gcttatgcga acgtgcggga agcggtgggc gacgagatcg
     8221 acatcgcggt gcactgccat aacgagttcg acacgccgag cgccatcggg gtcgcgaagg
     8281 ccgtagagcc ggtcaatcct ctgttctatg aagacgcgct aaacgtgccg ttctccgaag
     8341 gctggatggc gttgaagcgt tcaacgcgca ttccgatcct gacgggtgaa aagctggaac
     8401 tggtgcgcgg ttttcgcccc ttcctggaca cgcaggcggc tgacattgtg catccggatc
     8461 tggcgtttgc cggaggtatt actgggacaa agaagatcgc cgattatgcg gctctaacgc
     8521 ggaccccggt ggccctgcac aacgtagggt cgctggtgct gtgttacgcc aacgcgcatt
     8581 tcggggcatc gatccagaac ttctatcgct cggaaagcgc tctggggcgg ccgaaccggt
     8641 acgtcgagag tatggcggcg agcaatcccc ccgaggtgca caacgggcag ctcaaggttc
     8701 cggaggggcc tggcctgggg cttgacttga acgaagacgt gctgcgcagc aacctcgcgc
     8761 cgggcgagga atactggagt tgactgccta catccgaagt acgggactct ggatcagacg
     8821 ttttccggta cgacgaatgg ccggcggtag tcgcgtgtaa acatggcgtt cgcttcgtcg
     8881 tcaccggtgc accggtacgc ggcggcatcg aagtggagcg tgc
//
LOCUS       DATIFQ010000425        19580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1672977, whole genome shotgun sequence.
ACCESSION   DATIFQ010000425 DATIFQ010000000
VERSION     DATIFQ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19580
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672977"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VLE22_21215"
     CDS             <1..1414
                     /locus_tag="VLE22_21215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16985.1"
                     /translation="GTTVDAVPISYAAVNAASSTQVSAILPSNTPNGDGTLVLSFNNQ
                     PSAAFNVKVVEHSFGLYTLNSAGSGPAVMTDADYNVNFLTQSMKPGQVIILWGTGLGA
                     VTGNEFQGPLPGDLPYDVKVYVGGKLANVLYRGRSGCCSGLDQIVVEIPQGVDGCYVP
                     LVVSVDGVVSNFTTISVAANGGACSAQGFVTSQELQDARTRDSLRLGTIFLSHLSFKF
                     SIPGLGDITFGTDSGSADFHKYDFNQLIRSQGTSGFSSIGSCSVFVYQGDDPQNTDPI
                     VGTGLDAGNFIGLTGPNGPKQLTKQATGAYSARLGGGSPFGGDPLYLDPGPYTATGTG
                     VTDVKAFTAQLSLPAAVTWSNQDTISDIDRSKDLLITWEGGDPSTYVTIYGASSIEVP
                     KPAGAGFLCLERADKHQFSIPSVVLSALPATGASAEGIPLGFMLVGNTTTPARFPAEG
                     LDVGLFFGSSLSGKGVNFKE"
     gene            1487..3049
                     /locus_tag="VLE22_21220"
     CDS             1487..3049
                     /locus_tag="VLE22_21220"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSB16986.1"
                     /translation="MDHILSIVLFTPLVGFFVLLFIPSSKPQAVKLWANIASFAGFAV
                     CLPLVFQFDRTKDFQFVEKADWIPSIGASYHLGIDGYSLLLVVLTGLLGFLSIMSSWK
                     VIEERLKEYYAYFLILQVGMLGVFMSLDFLLFFVFWELVLVPMYFIIAIWGGARRVYA
                     AIKFIIYTLIGSVLMLLGILTLYFQHYSQTQIYSFDITALMQTSVSTTMQWWVFWAFF
                     VGFAVKVPMFPLHTWLPDAHTEAPTAGSVVLASVLLKMGTYGFLRYSLPLLPDAAKDP
                     TVIQIMAFLSIVAIVYGALVCLMQKDWKKLIAYSSVSHLGFCTLGIFALNPAGIAGSI
                     LQQINHGISTGMLFLIVGIVYERRHTREIAEYGGLLKAMPVFTIVFLITALSSMGMPP
                     LNGFIGEITILKGAYQASIWWAVAVVAGIVFGAAYLIWLFQRTMLGEISDKNAKLQDL
                     NLREIAVFAPLLVAAFWIGVYPKPFFDVMERPVAQIVERVQPGYHAKHNLPNPLNSSG
                     TAQLSAPIPGAR"
     gene            complement(2997..4082)
                     /locus_tag="VLE22_21225"
     CDS             complement(2997..4082)
                     /locus_tag="VLE22_21225"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB16987.1"
                     /translation="MNRAMRAWNRARLLWGYLRGHSGLSALPVEYIVETTAKCNLYCP
                     MCPRETHKQPKEDMPAEIFDRLVRESGRSAEHMMLIGLGEPFLDPLIFDRIEYCARHD
                     ISTLLSTNGTLLDEETSARLLQSSLEHVTLSFDGATRESFEYYRKGARFEKVRDNFVR
                     FARMKKERGSRMQVVVQMVRMERNAGEVENFVRFWKGVPGVDQVRIKEDETNLMHPLA
                     GHEAEEWKHPCHYLWRGPLYVKHNGDVYPCCQSYMLDGKPVGHIGERPLTEIWNSEEM
                     VRMRRLHVAGRAGEIDICSRCCTTIPHPALVVGSLLFHGRIVRRVLPVIERLVYFSKL
                     PKRLLQPPKPPNARQELVQIVGPSRKS"
     gene            4116..5525
                     /locus_tag="VLE22_21230"
     CDS             4116..5525
                     /locus_tag="VLE22_21230"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HSB16988.1"
                     /translation="MDLKNRIRILNSQPVRPGARYVLYWAQMNRRVEGNHALLHAAEL
                     ANSLGLPVLYYEGLTCSYPYANDRLHTFILEGVPDTEQRLRKLGIGYVFHLRRRQSDP
                     DDVLYRLAKDAAAVVTDDYPTFVAARHNASVPAKVGVAYHVVDSSCVVPMGLFGKREY
                     AAYTIRPKIRRVLGEHLVPPGPLKVRARFADPVAPFHTTVDEARIPRLVAECEIDHSV
                     PPSISFHGGRTQAERHLERFVRDNLRRYARHRNEPSAHATSDLSPYLHFGNISSIEVA
                     LRVGEHASEHKLVADEYLEELIVRRELAFNFARHTPPPYTLECLPDWARATLAKHGRD
                     ERSPCYTCEQFERAETHDELWNATQKELLLRGKIHGYYRMYWGKKVIEWSAAHEDALE
                     TMIHLHDRYALDGRDPNTYANILWCFGLHDRPWSERPVFGTVRYMSLDGMKRKTDVGA
                     YLAEIAQLERTGQDPFRLQ"
     gene            5533..6432
                     /locus_tag="VLE22_21235"
     CDS             5533..6432
                     /locus_tag="VLE22_21235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HSB16989.1"
                     /translation="MNFTVTGATGFIGRRLVRRLLAEGHSVHVLSRKVRAGFAPEVRS
                     SAWDPEREDPPADSLSTADAVIHLAGAPVAQRWTPIAKGRIRESRVEGTKRLVGALAA
                     LSKRPQALVSASAVGYYGSRGDEVLSEASSPGRGFLSEVCIQWENSAMEAEPLGVRVV
                     RLRIGVVLGKEGGALARMLPAFQWGMGGRLGSGKQWMSWIHVEDLVSLMLFAAQSPNL
                     RGPVNATAPNPVNNAEFTAILADVLRRPALIPAPAFAIRTLFGEMAEILLDGQRVLPQ
                     TALAAGFEFRHPGLKSAIQDLLA"
     gene            6445..8013
                     /locus_tag="VLE22_21240"
     CDS             6445..8013
                     /locus_tag="VLE22_21240"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HSB16990.1"
                     /translation="MRIALIVALAVTLVFVMIAIPQTGGFREMSLAELRDRIEGGWAG
                     QMIGVSFGAPTEFRSNGKILEGELPKWTPDRISNSINQDDLYVDMTFAKVLDDKGVDA
                     TTEDFGAMFRDAKYALWHANLASRRALKRGVPAALAGTPKYNAHANDIDFQIEADFIG
                     LMAPGLPQSCNLIAERAGKVMNYGDGIYGGMFVSGMYSAAFFEKDPHKIVEAGLATMP
                     AKSPYYMLIADVLAWHKQYPNDWRKVWQLIQDKWDKRDPCPAGALRPFNIDAKLNGAY
                     IALGLLFGDGDFGKTIEISTRAGQDSDCNPASSGGILGVMLGYKAIPVLWKSGVPAIA
                     DQKFRYTDFTFRTIVDSTEKRAIALVKKNGGRVDGQKLIVKTQQPVAAKLAVWDDYGS
                     PVERIASGDRRWQWKGEWRANESKRRGTEVQTRTASAKGAEAVVTFEGTGAIVTGPYL
                     SNGGKAEVYLDGKLHGTVDVYSDEEASKGEESVWHAFGLKNGKHTVRLVVLGAPYPGS
                     KGSDVGIDDVVVFR"
     gene            8085..8294
                     /locus_tag="VLE22_21245"
     CDS             8085..8294
                     /locus_tag="VLE22_21245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB16991.1"
                     /translation="MGIPNVKRFRPFCPECKENFTLMVVLPKDADVDAYLRDAVVRHD
                     HKAYQESKQAHFKIRVGQQKQRRAK"
     gene            complement(8311..9594)
                     /locus_tag="VLE22_21250"
     CDS             complement(8311..9594)
                     /locus_tag="VLE22_21250"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB16992.1"
                     /translation="MSAVLLSLLATSLMAQDPNSSDPKERRRAAEEMARKGAEAIPTL
                     QKMLSDPDLDVRIEAVKSIVVIDTEKSLEPLVRATRDSDPEIQVRATDGLVNFYLPGY
                     VRSGLSATLRRAGRQIKGKFTDTNDQVIDLYLEAKPEIVEALGKLARGGASMESRANA
                     ARAVGVLRGKSAVSDLIAAIKSTKDSQVMFECLIAFQKIRDRSAAPQLTFLLRDLDER
                     VQVAAVETQGLLYNMDALPRLREVLARTDKKEVRRAALRSIAMLPDPPNRPVLQRYLQ
                     DKDDELREAAAEGLGRIGDTQDLPVLEKRFAEETKNSPRVSAAFALVMMGRTEMSEFS
                     PLRYLVNTLNSSSRAMEARALLIEAARKPEVRRTLHDSMIRGSSDEKIYLAQVMAASG
                     DSEQLPLLEQLSRDPDVKVAQEGLRALRVLKARVQ"
     gene            complement(9673..10518)
                     /locus_tag="VLE22_21255"
     CDS             complement(9673..10518)
                     /locus_tag="VLE22_21255"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HSB16993.1"
                     /translation="MDSSSLHEVVCAHSPDSDDAFMFYALATRKIRSRLVRFRHELND
                     IETLNRSAAEGRYELTAISYHAYPHIADKYVLMTSGSSVGDGYGPLVVAGRPLQPAEL
                     KGKRIAVPGLMTTAYLTMKLLEPDFEEVVTPFDRILDAVHDRSVDAGLVIHEGQLTFA
                     SGGFHSVIDLGAWWKQHTGLPLPLGANALRRELPPEIRSECCRMMRESIQYAFDHHDE
                     ALEYALQFAREMDQRLADKFVGMYVNHYTLDCGTVVPQAAQKLLDMGFDAGLVPQKVS
                     LEFVR"
     gene            complement(10584..11228)
                     /locus_tag="VLE22_21260"
     CDS             complement(10584..11228)
                     /locus_tag="VLE22_21260"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB16994.1"
                     /translation="MRTSSLIVLLLVVVVAAAAAEPGFVTLFDGKTLNGWILVGKHGR
                     GYVPENGILVCPADGGGNLYTEKEYSDFAFRFEFRMEPGGNNGVGIRAPREGDAAFVG
                     MEIQILDDGNERYKGQLKPTQYHGSIYDVIPARPGFLKPAGEWNAEEIVAKGRNIRIT
                     LNGVIIVDANLDIVKEPEVLKRHPGLARKSGHVGFLGHDTRVEFRNIRIRDLAN"
     gene            complement(11257..12651)
                     /locus_tag="VLE22_21265"
     CDS             complement(11257..12651)
                     /locus_tag="VLE22_21265"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB16995.1"
                     /translation="MTEQRTINRRGFLAAASAALRGGSAHAATRLPNIVMIYCDDLGY
                     GDLGCYGSAIRTPNLDRMAAEGTRFTHFYSGNPVCSPSRAALMTGRYPTRAGVPRVLF
                     PTDTTGLPDSEVTMAQVLKTKDYRTMCVGKWHLGHLPPYLPTSRGFDGYFGIPYSNDM
                     TPRPLLRNTETIEEPAKLETLTPRYTEQAVRFVEQSKDSPFFLYFAHTYPHIPLAASS
                     RFRGRSPLGLYGDVVEEIDWSVGEVLSTLRKRGLEENTLVMFSSDNGPWYQGSPGRLR
                     GRKGMTWEGGQRVPFIARFPGRIQVGRTCQGVASVMDILPTVSRLCETPRPRNPLDGI
                     DIWPLLSGEKTELDREALLYFDNVNLQCARWKRWKLHVARHNSAAYSPAPQGGRVNLP
                     LRPPELYDVVNDSDESYDIAAENPKVVAEIEARIERLMRGFPDDIRTAWAKTKATPTA
                     PSSTGTFPRPANKQ"
     gene            12922..13587
                     /locus_tag="VLE22_21270"
     CDS             12922..13587
                     /locus_tag="VLE22_21270"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HSB16996.1"
                     /translation="MSAGYFCVSCRTPFLNSSPLDENGRCALCRNGLRGFDAAYTFGS
                     YEGALRELIHLFKYGGVRTLAKPFGGHLAVALPRNEQFDAVVPMPLHWLRRWRRGFNQ
                     SDLLAREVARRCGVPVIHAVRRRRSTLSQAGLTNAKRRANVAGAFAPKQGRPLEGKRV
                     LLIDDVMTTGATAAACALAIKRAGARYVALLTLARTDRRSMAPVRIPSTADGGLSVES
                     ASC"
     gene            13581..14108
                     /locus_tag="VLE22_21275"
     CDS             13581..14108
                     /locus_tag="VLE22_21275"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSB16997.1"
                     /translation="MLNGNRLHKPVLVLNASYEPINVCAARRAIVLILKGVATAEEHS
                     HARVHSAFDTYRLPSVIRLLEYRRIPHQTRALSRKNILMRDRYTCQYCLRTLPSSELT
                     LDHVIPRSRAGETSWENLVTCCHPCNNRKGNRTPEESGLKLVRLPRPFSLHTSRHLMR
                     LLGKGDDQWRKYLFY"
     gene            complement(14102..14719)
                     /gene="infC"
                     /locus_tag="VLE22_21280"
     CDS             complement(14102..14719)
                     /gene="infC"
                     /locus_tag="VLE22_21280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSB16998.1"
                     /translation="MIPRRPFIPRRPRIRENVNEAIRAREVRAVFPDGSVEILPTSQA
                     LRRAQELGLDLIVVAPTAEPPVAKAMDYGQWQYEQKKKQQEAKKKQHVIQVKELKFRP
                     NTDDHDYDFKKNHAIRFLKEGNRVKAVVQFRGREIAHVDLGKNLLMRFVKDLAEWGAV
                     EGQPRLEGRFAHVIISPVKAAAKKEGKPQPSSGSPPGPQPPPAVQ"
     gene            14845..16449
                     /locus_tag="VLE22_21285"
     CDS             14845..16449
                     /locus_tag="VLE22_21285"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HSB16999.1"
                     /translation="MRPLTLLLAWACLTVSAAEDGAAIYKQHCAGCHEPETAGQTPDR
                     NALARMSYDAILTTLDTGSMKSLGADLTPAERRAVSRFLARRASGGPPRTMRLDYFHT
                     GNARQESFAVDGLVLEGPWPGNPRRALDTTGLGKYFFEMVDGASGRALYSRGFASIFG
                     EWELTPEAKEKRETFHESLRFPAPSKPVRVVLKKRGSRNEWREIWSVPVDSSGPSAES
                     VGVSSSARVWAVTKNGEPQDKVDVLFLGDGYTATDMEKWHGDARRVAELLFAESPFKE
                     RKRDFNIWAIDTPAGESGVSRPSDGVRRSSPIGASYDAFGSERYVLVFDNKRLREIAS
                     AAPYEFLAIIVNERKYGGGGIFNLYATSAADNEFTPYVFVHEFGHHLAGLADEYYTSD
                     VAYESTADRPEPWEPNVTANPKASRWKELLTPGIPLPTPWSRDEFESSQKNVQEKRRA
                     IRSRNQPESEMEALFREERGHLTRLLKAERYAGKVGAFEGAMYEPRGYYRPQVDCIMF
                     TRDEVGFCAVCRRAIERVIDLYTRGQ"
     gene            16452..17825
                     /locus_tag="VLE22_21290"
     CDS             16452..17825
                     /locus_tag="VLE22_21290"
                     /inference="COORDINATES: protein motif:HMM:NF019260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HSB17000.1"
                     /translation="MPLLCRCLILTILTAVVGLTAQPGAKPRLLVLTDIGGDPDDTQS
                     LIRLMTYSNEFDIEGLIASAAGTPGELGVDIVKPELIREVVEAYGRVCGTLSLHAAGY
                     PPVERVLDCIKSGNPKRGVGQLGHDKDTEGSRWIIKLADRPDPRPLNIAIWGGSTELA
                     QALWRVRNDCGRDGLNRFVGRLRVHSIAHQDDTGPWIIANFPELFYVLSMEPDGVDRR
                     QGAYRGMYLGGDESLTSLDWVNAHVKTDHGPLGALYPLKTWTAPNPHGVLKEGDTPSW
                     FYFLPNGLGDPEHPEWGGWGGRFRRASGRLYRDAQDSTASVTDARATVWRWRPAYQND
                     FQARMDWCVKGYREANHPPLVVLDSKPGRDVVRKQTRPGANVPLNAAGSSDPDGDHVS
                     YRWWIYREAGRLSEQAVIERAERERATVRIPSGSAGAEIHVILEATDSGAPPLISYRR
                     MILDVRP"
     gene            complement(18373..19329)
                     /locus_tag="VLE22_21295"
     CDS             complement(18373..19329)
                     /locus_tag="VLE22_21295"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HSB17001.1"
                     /translation="MPMQGSLSVERMCQLAQVSRAGFYRSLKEQLPAEEDMEVRSAIQ
                     EIALQHRRRYGYRRITIELRRRGMLVNHKRVARIMREDNLLAIQPRAFVVTTDSDHKL
                     EVFLNLASRMKLTGINQLWVADITYIRLKAEFVYLAVVLDGHSRKVVGWALERTLAAR
                     LPLAALARALAERRPLPGLVHHSDRGVQYASGDYVRILRKHQMIPSMSRPANPYDNAS
                     CESFIKTLKREEIDANQYRDLEHLRANVEEFIEQYYNRYRLHSALGYRSPEEFEQVAA
                     DVCSAGARMSFFRHEEIYRSGAQREPTEAGPLTHRLDESPTD"
     gene            complement(19329..>19580)
                     /locus_tag="VLE22_21300"
     CDS             complement(19329..>19580)
                     /locus_tag="VLE22_21300"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB17002.1"
                     /translation="ELGIHRTQLYKWRDQMEPVEDGPGPAANSRERELRKEIRELKRV
                     LGEKVLEVDFFKGALQKVEARRQNSSSSGAKASTSRSES"
BASE COUNT         3616 a         6120 c         5985 g         3859 t
ORIGIN      
        1 cggcacaacg gtggatgccg taccgatctc ctacgcggcc gtcaatgcgg cgtcttcgac
       61 gcaggtatcg gcgatcctgc cttcgaacac gcctaatggc gatggcacgc tcgtcctctc
      121 cttcaacaac cagccgagcg ctgcctttaa cgtcaaggta gtcgagcaca gcttcggcct
      181 ttacactctc aacagcgcgg gcagcggccc ggctgtgatg accgacgcgg actataacgt
      241 caactttctg acacagtcaa tgaaaccggg tcaggtgatc attttgtggg gcaccggact
      301 gggtgcggtg acggggaatg agtttcaagg accgcttccg ggcgatctgc catatgatgt
      361 gaaggtttac gtcggcggaa agctggccaa cgtcctttac aggggccggt ccggatgttg
      421 ctccgggctc gatcagatcg tcgttgagat cccgcagggt gttgacggat gctatgtccc
      481 gttagtggtg tctgtagacg gggtagttag caacttcacc acgatctcgg tggcggcgaa
      541 cggcggtgcg tgttcagccc agggattcgt gacgtcgcag gaactccagg atgcgcggac
      601 ccgagactcg ctccgcctcg ggaccatctt cctgtcgcac ctcagcttca aattctcgat
      661 accaggcctg ggcgatatca cgttcggcac cgactctggt agcgccgact ttcacaagta
      721 cgacttcaac cagttgatcc ggtcacaggg aacttcggga tttagctcga taggctcgtg
      781 cagcgtgttc gtctaccaag gcgacgatcc ccagaacaca gacccgatcg tcggcacggg
      841 gctggacgcc ggcaacttca tcggcctgac cggaccgaac ggcccaaagc aactcaccaa
      901 gcaggcgacg ggagcctact ccgcccggct gggcggcggc tcgcccttcg gcggtgatcc
      961 gttgtacctg gacccgggcc cgtacacggc aacagggacg ggtgtcaccg acgtgaaggc
     1021 gtttaccgcc caattgagtc tgcccgcggc cgtaacctgg tccaaccagg acacgatttc
     1081 ggacatcgac cgctcgaaag atctgctgat cacctgggag ggtggcgacc cgagcaccta
     1141 cgtcacgatc tacggcgcgt cgtcgatcga agttccgaaa ccggcgggcg cggggttcct
     1201 ttgcctcgag cgagccgaca agcaccagtt cagcatccct tcggtcgtcc tgtcggcgct
     1261 gccagcgact ggggcctccg ctgagggcat cccgctcggc ttcatgctgg tcgggaacac
     1321 gaccaccccg gcgcggttcc cggccgaggg cttggatgtc gggctgtttt tcggcagctc
     1381 actcagcggc aagggcgtca actttaagga gtagcgcgcc cggtttgccg cgagccgcgg
     1441 ccatccggta agataagggg cttgtccgct cgccggcggg ccccttatgg accatatcct
     1501 ttcgatcgtt ctatttaccc cgctggtcgg gttctttgtc ttgctgttca tcccatcctc
     1561 gaaaccccaa gcggtcaagc tatgggcgaa catcgcgagc ttcgccggtt tcgccgtgtg
     1621 cctgccgctg gtgtttcagt ttgaccggac gaaggacttc cagttcgtcg agaaggcgga
     1681 ctggatcccc tccatcggcg cctcgtatca tctggggatc gacgggtaca gcctgctgct
     1741 cgtggttctg accgggctgc tcgggttcct ctcgatcatg tcgagctgga aggtcatcga
     1801 ggagcggctc aaggaatact acgcgtactt cctgatcctc caggtgggca tgctcggcgt
     1861 cttcatgtcg ctcgacttcc tgctgttctt cgtgttttgg gaactggtgc tggtaccgat
     1921 gtacttcatc atcgccattt ggggcggagc caggcgcgtg tacgccgcga tcaagttcat
     1981 catttacaca ctgatcggct ctgtgctgat gctgctcggc attctgaccc tgtacttcca
     2041 gcattacagc cagactcaaa tctacagttt cgacatcacc gcgttgatgc aaaccagcgt
     2101 cagcacgacg atgcagtggt gggtgttctg ggcgtttttc gtcggcttcg cggtgaaagt
     2161 gccgatgttc ccgctccaca cctggctgcc ggacgcgcac acggaagcgc cgaccgccgg
     2221 ctcggtggtc ctggccagcg tcctgctgaa gatggggaca tacggcttcc tgcgctattc
     2281 cctgcccctg ttgcccgatg cggccaaaga cccgacggtt atccagatca tggccttcct
     2341 atcgatcgtg gccatcgtct acggagcgct cgtgtgcctg atgcagaagg actggaagaa
     2401 actgatcgcg tactcatccg tgagccacct gggtttctgc accctcggca tcttcgcgct
     2461 gaatccggca ggcatagcgg ggtcgattct tcagcagatc aaccacggaa tctcgaccgg
     2521 gatgctcttc ctgatcgtcg gcatcgtgta cgagcggcgt cacacccgcg agatcgcgga
     2581 gtacggcggc ctgttgaagg cgatgccggt gttcaccatc gtcttcctga tcacggcgct
     2641 tagctcgatg gggatgccgc cgctcaacgg gttcatcggt gagatcacca tcctgaaggg
     2701 agcctaccag gcgtcaatct ggtgggcggt ggccgtcgtg gccgggattg tgttcggcgc
     2761 cgcgtatctg atctggctgt tccagcggac gatgctcggg gagatctccg acaagaacgc
     2821 gaaactgcag gacctgaacc tgcgcgaaat cgcggtgttc gccccgctgc tggtggcggc
     2881 gttctggatc ggcgtctacc cgaaaccctt cttcgacgtg atggagcggc cggtcgcgca
     2941 aatcgtggag cgcgtgcagc cgggctacca cgcgaagcac aacctgccga atccgctcaa
     3001 ctcttccggg acggcccaac tatctgcacc aattcctggc gcgcgttagg cggcttcgga
     3061 ggctggagca accgtttcgg cagcttcgag aaataaacga ggcgctcgat gaccggcagg
     3121 acacgccgca cgatccgccc gtgaaacagc aggctgccga cgaccagcgc aggatgtgga
     3181 atcgtcgtgc agcaccgcga gcagatatcg atctctcccg cccggccagc gacgtggagg
     3241 cggcgcatcc gcaccatttc ctccgagttc cagatctccg tgagcggccg ctcgccgatg
     3301 tgcccgaccg gtttgccgtc gagcatgtag ctctggcagc acgggtagac gtcaccattg
     3361 tgcttcacgt acagcggccc gcgccagagg taatggcaag ggtgtttcca ctcttccgcc
     3421 tcgtgtccgg ccagggggtg catcaggttg gtttcgtctt ccttgattcg gacctggtcg
     3481 acgccaggca cccctttcca gaaccggacg aagttctcga cctcaccggc gttgcgttcc
     3541 atgcggacca tctggacgac gacctgcatc cttgatccac gctctttctt catccgcgcg
     3601 aagcggacga agttgtcgcg gactttctca aagcgcgcgc ccttgcggta gtactcgaag
     3661 ctctccctgg tggcgccgtc gaaactgagg gtgacatgtt cgagggacga ctgcaggagc
     3721 cgagcggatg tttcctcgtc gagcagggtg ccgttggttg acagaagggt ggagatatcg
     3781 tggcgcgcgc agtactcgat ccggtcgaat atcagcggat cgaggaacgg ctcgccgagc
     3841 ccgatgagca tcatgtgctc ggctgagcgg ccggattccc gaacgaggcg gtcgaaaatc
     3901 tcggcgggca tgtcttcttt gggctgctta tgggtttcgc ggggacacat cgggcagtac
     3961 aggttgcact ttgccgtcgt ctcgacgata tactcgaccg gcagggcgct caacccgctg
     4021 tggccgcgca agtagcccca aagtagtctg gcccggttcc aggcgcgcat tgctcgattc
     4081 atttattcta gcggccgggg ggaggacagc gtgtaatgga cttgaagaac cgcatccgca
     4141 tcctgaattc gcagcccgtc cggcctggcg cacgctacgt gctctattgg gcacagatga
     4201 accgccgggt ggaggggaac catgcgctgc tccacgccgc ggagctggcc aacagcctcg
     4261 gcctccccgt tttgtactac gaagggctca cctgctcgta cccttatgcg aacgaccgct
     4321 tgcacacgtt catcctggag ggcgtgccgg acacggaaca gcggctgcgt aagctcggca
     4381 ttgggtacgt cttccacctg cgccggcggc agtccgatcc tgacgatgtg ctctacaggc
     4441 tagccaagga cgccgccgca gtcgtcaccg acgactaccc gacgtttgtt gctgcgcgtc
     4501 acaacgcctc ggttccggcg aaggtgggtg tcgcctacca tgtggtcgac tccagttgcg
     4561 tcgtgccgat gggccttttc ggaaagcgag agtatgctgc ctacacgatt cgcccgaaga
     4621 tccgccgtgt gcttggcgag catctcgtcc cgccgggacc gttgaaggtg cgggcgcgtt
     4681 ttgcggatcc cgtcgcgccg ttccacacga cggttgacga ggcgcgcatc ccgagactgg
     4741 tagcggaatg cgagatcgat cacagcgtcc caccctcgat ctcgtttcac ggcggccgca
     4801 cgcaggccga gaggcacttg gagcgatttg tcagggacaa tcttcgccgg tatgcccgcc
     4861 atcggaacga gccttcggcg cacgcgacct ccgatctgag cccatacctg cactttggga
     4921 acatctcgtc gatcgaagtc gctctccggg tcggggaaca cgcatcggaa cacaagctgg
     4981 tcgcggatga gtacctggag gagctgatcg tccggcgtga gctcgcgttc aatttcgccc
     5041 gccacactcc gccgccgtac acgctcgaat gcctgcccga ttgggctaga gcgacgctgg
     5101 ctaaacacgg ccgcgacgaa cggtctccct gctacacgtg cgagcagttc gaacgggcgg
     5161 aaacgcacga cgaactctgg aacgccaccc agaaggaact gctgctccgc ggcaagatcc
     5221 acggctacta ccggatgtac tgggggaaga aggtgatcga gtggtcggct gcccacgagg
     5281 atgcgctcga aacaatgatt cacctgcacg accgctatgc gctcgacggc cgtgacccga
     5341 acacgtacgc taacatcctt tggtgtttcg gcctgcatga tcgcccgtgg agtgagcggc
     5401 cggtcttcgg aaccgtccga tatatgtcac tcgacggcat gaagcggaag acggacgtcg
     5461 gcgcttacct cgccgagatt gcacagctag agcggaccgg gcaggatcca tttcgcctac
     5521 aatagcggac aagtgaactt cacagtcact ggggcaaccg gatttatcgg ccggcggctt
     5581 gtccggcggc ttctggccga aggccactcc gttcacgtcc tttcgcgaaa ggttcgggcc
     5641 ggctttgccc cggaggtccg gtcgtcggca tgggatcccg agagggagga cccgcccgcg
     5701 gattcccttt cgacggccga cgcggtgatt cacctggccg gagcgccggt cgcgcagcgc
     5761 tggactccca tcgcgaaagg ccgtatccgg gagagccgcg tggagggtac gaagcggctc
     5821 gtcggtgcgc tggccgcgct ctcaaagcgc ccccaggcac tggtcagcgc ctcggcggtc
     5881 ggctattacg gctcgcgagg cgacgaggtg ttgtccgagg catcttcgcc cggccggggc
     5941 ttcctctcag aggtttgcat ccaatgggag aactccgcga tggaggcgga accgctcggg
     6001 gtccgggtgg tgcggctgcg gattggagtc gttctgggca aagaaggcgg tgcgctcgcg
     6061 cgcatgctgc ctgccttcca gtggggaatg ggcggacggc tggggtcggg caaacaatgg
     6121 atgtcgtgga tccacgtgga ggaccttgtc agcctgatgc tgtttgccgc gcaaagcccg
     6181 aatctgcgcg gaccggtgaa cgccaccgcc ccgaatcccg tgaacaacgc agagttcacg
     6241 gcaatcctgg cagacgtgct gcgccgtccg gccctgattc ctgcgcccgc cttcgcgatc
     6301 cgaaccttgt ttggcgagat ggccgagata ctgctggacg gtcagcgggt gctgccgcag
     6361 accgcgctcg ccgccggttt tgagttccgg catcccggcc tgaaatccgc cattcaagat
     6421 ctgctcgcgt agtaaaatcc gaggatgcga attgcattga ttgtagcgct cgctgtcacc
     6481 ctcgtcttcg tcatgattgc catcccacag acaggcggct ttcgagagat gtccctcgca
     6541 gaacttcggg accggatcga ggggggctgg gccggtcaga tgatcggagt cagcttcggc
     6601 gctccaaccg aattccggtc gaacggcaag atcctcgagg gcgagctgcc caagtggacg
     6661 ccggaccgca tcagcaactc gatcaaccag gacgacctct acgtcgacat gaccttcgcg
     6721 aaggtgctcg acgataaggg cgtcgacgcc acaacagagg attttggcgc aatgttccgc
     6781 gacgcgaaat acgcgctgtg gcacgccaac ctggcctcgc gccgggcgct caagagaggc
     6841 gttccggcgg ctctggcggg taccccgaag tacaatgcgc acgccaacga catcgacttc
     6901 cagatcgagg cggacttcat cggcctgatg gcgccgggtc ttccccagtc ctgcaacctg
     6961 atcgccgagc gggccggaaa ggtgatgaat tacggcgacg gcatctatgg aggcatgttc
     7021 gtctccggga tgtactcggc ggcgttcttt gagaaagacc cgcacaaaat cgtcgaagcc
     7081 ggactggcga cgatgccggc gaagagccca tactacatgc tgattgccga cgtcctcgcc
     7141 tggcacaagc agtatccgaa cgactggaga aaggtctggc agctcattca ggacaagtgg
     7201 gataagcgcg atccatgccc ggccggcgcc cttcggccgt tcaacatcga cgccaaactg
     7261 aacggcgcat acatcgcgct tggtctgctc ttcggagacg gggacttcgg caagacgatc
     7321 gaaatctcga cccgggcggg tcaggactcc gactgcaacc cagccagttc cggcggcatt
     7381 ctcggcgtga tgctcggcta caaagcgatt ccggttctct ggaagagcgg cgtcccggcg
     7441 atcgcggacc agaagttccg ctacacggac ttcacgttcc gcacgatcgt ggatagcacc
     7501 gagaagcgcg cgatcgctct cgtcaagaag aacggcggac gcgtggacgg gcagaagctg
     7561 atcgtgaaga cgcagcagcc ggtggccgcg aagctggccg tttgggatga ctacggctcg
     7621 ccggtcgagc gcatcgcaag cggtgaccgg cgctggcagt ggaaagggga atggcgggcg
     7681 aatgagtcca agcggcgtgg aacagaggtt cagacacgaa ctgcgtccgc gaaaggcgcg
     7741 gaagcggtgg tgaccttcga aggcaccggc gcgatcgtaa ccggtccgta tctctccaac
     7801 ggcggcaagg cggaagtgta tctcgacggg aaactgcacg gcaccgtgga tgtctactcg
     7861 gatgaggagg cttccaaggg cgaagagtca gtctggcacg cattcggtct gaaaaatggc
     7921 aagcacaccg tgcgattagt cgtcctcgga gcgccatacc cgggcagcaa gggcagcgac
     7981 gttggcatcg acgacgtagt agtgtttcgg tagacgcagg ctacttggcc actccctcac
     8041 gttcgtggtt ctgcgggctc cggctgtgtt agcatgagaa agttatggga attccgaatg
     8101 tgaagcgctt ccggccgttc tgtccggaat gtaaggaaaa cttcacgctg atggttgtcc
     8161 tccccaagga cgccgacgtc gacgcttatc tccgggacgc tgttgtacgc cacgatcaca
     8221 aagcgtacca ggagtcgaaa caggcgcact tcaagattcg tgtcggccag cagaagcagc
     8281 gtagggccaa gtaagagccg atccttctct ttattgcacg cgggctttca acacacggag
     8341 agcccgcaag ccttcctgag ctaccttgac atccgggtcg cgtgagagct gctcgaggag
     8401 aggaagttgc tcgctgtctc cgcttgccgc catgacctgc gcgagataga ttttctcgtc
     8461 cgaactcccg cgaatcatcg agtcgtggag ggtccgcctc acctccggct tacgggcggc
     8521 ctcgatcagc agcgctctgg cctccatggc gcggctgctc gaattcaggg tattcaccag
     8581 atagcgtaac ggactgaatt cggacatttc ggttctgccc atcatgacca aggcgaaggc
     8641 ggcggacacg cggggagaat tcttcgtctc ttcggcgaag cgcttctcca atacgggcag
     8701 gtcctgcgta tctccgatgc ggcccaggcc ttcggctgcc gcctcgcgaa gttcgtcatc
     8761 cttatcctgc aggtagcgct gaaggacagg acggttgggc ggatcgggca acatcgcgat
     8821 ggagcgaagc gccgcgcgcc gcacctcctt cttgtccgtt cgcgcgagca cttcccgcaa
     8881 gcgagggagc gcatccatgt tgtaaagcaa gccctgggtc tccacggccg ctacctggac
     8941 gcgttcgtcc agatcacgga ggaggaaggt caactgcggc gcggcgctcc ggtcgcggat
     9001 cttctgaaac gcgatcagac actcaaacat cacctggcta tccttggttg atttgatggc
     9061 cgcgatgaga tcagagacgg ctgatttccc gcgaagaacg ccgaccgcac gcgccgcgtt
     9121 ggcccgggac tccatcgagg caccgccgcg ggcaagtttt cccagcgcct ccacgatctc
     9181 gggtttcgcc tcgaggtaca gatcgatcac ttgatcgttg gtgtcggtga acttcccctt
     9241 gatctgtctg cccgcccggc gcagggtcgc ggagagtcca ctgcgcacgt aaccgggcag
     9301 gtagaaattc accagaccgt cggtggcgcg cacctggatc tctggatcag aatcgcgcgt
     9361 cgcccgcact agtggctcca ggctcttctc cgtgtcgatt acgacgattg acttgacagc
     9421 ttcgatgcga acgtcgaggt caggatcgga cagcatcttc tgcagcgtgg ggatcgcttc
     9481 cgcgcccttc cgggccattt cttccgcggc tcgacggcgt tctttcggat cggaactgtt
     9541 cgggtcttgg gccatcaggc tggtcgcaag gagcgagagc aatacagcgg acaaaacgca
     9601 cactcgtgcc atatcttcaa ggtatcgagg accttcgaat gtgcgcaacc gccggagccg
     9661 ggacgctgcg ggttacctga cgaactcgag tgagaccttc tggggcacca gaccggcatc
     9721 gaagcccatg tcgagaagct tctgcgccgc ctgtggaacc accgtcccgc agtcgagtgt
     9781 gtagtggttc acgtacatcc cgacgaactt atcggcgaga cgctgatcca tttccctagc
     9841 gaactggagc gcatattcga gcgcttcgtc atgatggtcg aatgcgtatt ggatgctctc
     9901 acgcatcatc cggcaacatt cgcttcgaat ctccggcggc agctcgcgcc gcaacgcgtt
     9961 cgcgccgagc ggaagcggaa gtcccgtgtg ctgcttccac cacgcgccga gatcgatcac
    10021 gctgtgaaat ccgccgctgg cgaaagtcaa ctgtccttcg tggatcacca acccggcgtc
    10081 gacgctgcga tcgtggacgg cgtccaaaat ccggtcgaac ggcgtgacga cctcttcgaa
    10141 atccggctcc aggagtttca tcgtcaggta cgcggtcgtc atcagaccgg gtaccgcgat
    10201 ccgtttcccc ttcagttccg ccggttgaag tggacgcccg gctaccacca gaggcccgta
    10261 cccatcgcca acgctcgagc cgctggtcat gagcacgtac ttgtccgcta tgtgtggata
    10321 tgcgtgatag gagatcgcgg tcagctcata acgcccctcc gccgcactgc ggttgagcgt
    10381 ttcgatgtca ttgagctcat gccggaaccg gacaaggcgg gagcggatct ttcgtgtcgc
    10441 gagcgcatag aacatgaagg cgtcgtccga atccggacta tgcgcgcaca cgacctcgtg
    10501 gagtgaagat gagtccatac gagacgtaag ttcccctcaa tcttaccaga cggagtgccg
    10561 tatccatcga tcgggcgatc gactcagttc gccaggtccc tgatccggat attccggaac
    10621 tcaacgcgcg tgtcgtgccc gaggaagccg acgtggccgc tcttgcgcgc aagtccagga
    10681 tgccgcttta gaacttcagg ttctttgacg atgtcgagat tggcgtcaac gataatgact
    10741 ccgttaaggg tgatgcggat gttccggccc tttgccacaa tctcttctgc gttccattcg
    10801 ccggcgggct tcaagaagcc gggccgcgcg gggatcacgt cgtagatcga cccgtgatat
    10861 tgggtcggtt tgagctgtcc cttgtaccgc tcgttcccgt cgtcgagaat ctggatttcc
    10921 attccgacaa acgcggcgtc gccttcccgc ggagcgcgga tgcccacgcc gttatttccc
    10981 cctggctcca tgcggaactc gaaccgaaag gcgaagtccg aatactcttt ttcggtataa
    11041 aggttcccgc cgccgtcggc ggggcacacc aggatgccgt tttccggcac atacccgcgc
    11101 ccgtgcttac cgacgaggat ccagccgttg agcgtcttgc cgtcgaacag cgttacaaag
    11161 cccggctcgg cggcagcggc agccacaacc accaccagca gaagaacgat cagactcgaa
    11221 gtacgcataa ggttacagtc tactgtacgc ccccgcctac tgcttgttgg cggggcgcgg
    11281 gaacgtaccg gtcgaggagg gggccgttgg agtggctttc gttttcgccc aagccgtccg
    11341 gatgtcgtcc gggaaccccc gcatcaaccg ctcgatccgc gcctcgattt ccgctaccac
    11401 cttcggattc tcggcggcga tgtcatagga ctcatctgaa tcgttcacaa cgtcgtacag
    11461 ctccggtgga cgcagcggaa ggttgacacg gcctccctgc ggcgctgggc tgtaggcagc
    11521 agaattgtgc cgggccacgt gcagcttcca gcgcttccat cgcgcgcatt gcaggttcac
    11581 gttgtcgaag tacagtaagg cctcgcgatc aagctccgtc ttttcgcccg agagaagcgg
    11641 ccagatgtcg ataccgtcga gcgggttcct gggacgcggc gtctcgcaaa gccgggatac
    11701 ggtcggcagg atgtccatca ccgatgccac gccctggcac gtcctcccga cctgaattcg
    11761 accggggaac cgcgcgatga agggcacgcg ctggccgcct tcccaggtca ttccctttct
    11821 cccgcgtagc ctgccgggac taccctgata ccaaggcccg ttgtcggagg aaaacatgac
    11881 cagcgtgttc tcttcgaggc cacgcttgcg gagcgtcgag agcacctcgc cgacgctcca
    11941 gtcgatctct tccaccacat cgccgtacag gccgagagga gatctccccc ggaaccggct
    12001 cgaggccgcc agcgggatgt gaggataggt gtgcgcgaag tagaggaaga acggcgagtc
    12061 tttcgactgc tcaacaaaac ggacggcctg ctccgtgtat cggggcgtca gcgtctcgag
    12121 cttcgccggc tcttcgatgg tctccgtgtt ccggagcagc ggccggggag tcatgtcgtt
    12181 gctgtaaggg atgccgaagt atccgtcgaa gccacggctg gtcggcaagt agggaggcag
    12241 gtggccgagg tgccacttcc cgacgcacat cgtccggtag tccttcgtct ttaatacctg
    12301 tgccatcgtc acttccgaat ccggcaagcc ggtggtatcg gtgggaaaca gcacgcgggg
    12361 caccccggca cgcgttggat accggccggt catgagggcc gcacgggagg gcgaacagac
    12421 tgggttcccc gagtagaaat gagtgaagcg cgttccttcg gcggccattc gatcgagatt
    12481 cggggtacgg atagcggagc cgtagcaacc aagatcgccg taacccagat cgtcgcagta
    12541 gatcatgacg atattcggca gtcgcgtggc ggcatgcgcc gagccacctc gaagcgcagc
    12601 cgaagcggcg gcgaggaaac cgcggcggtt gatggttcgt tgctcagtca tcgcgtcgat
    12661 cataccccaa cgagccacgg tccgaccggg cctgaggacg gggccaagtt tgttgtacac
    12721 taagacttac ggtggaggtg ggtcttctcc agtagctggc gattacacgt cctgcccgac
    12781 agcctaccgg gctacgtcgc cagactgcct tctgccttct tttcgctcct cttccccgac
    12841 gactgccgcc tgtgcggaga accgctccgg gatatcacac gcatccctgt ctgccggaaa
    12901 tgcctgagtc tgccggagcc cttgtcggcg gggtacttct gtgtcagttg ccgcacgccg
    12961 tttctgaact cctcgcccct ggacgagaat gggcgttgtg ctctctgccg caacgggctg
    13021 cgcggtttcg acgccgccta taccttcggg agctatgaag gcgcactgcg cgagctgatc
    13081 caccttttca agtacggcgg ggtccggacg ctggcgaaac cattcggcgg gcacctggcc
    13141 gtcgcgctgc cgcgaaacga gcagtttgat gccgtcgtac ccatgccgct gcactggctg
    13201 cgccggtggc gtcgaggttt caaccagtcc gatctgcttg cccgtgaggt cgcccgccgc
    13261 tgcggcgtgc ctgtcattca cgctgtccgg cgccggcgtt ccacactgtc tcaggccggt
    13321 ctcacgaacg ccaagcgccg tgccaatgtc gcgggcgcct ttgctccgaa acaaggcaga
    13381 ccactcgagg gaaaacgcgt actgctcatc gatgacgtga tgacaaccgg cgcgaccgcc
    13441 gctgcctgtg ccctcgccat caagcgcgcc ggcgcacgct acgttgcgct tctgaccctc
    13501 gcccgcaccg acaggcggtc catggctccg gttcggatcc cctcaacggc agacggcgga
    13561 ctttccgtgg agagcgcctc atgttgaacg gtaaccgact gcacaagccg gtcctcgtgc
    13621 tcaacgcgag ctacgagccg atcaacgtct gcgcggcccg gcgggcgatc gttctgattc
    13681 tcaagggcgt cgccacggcg gaggagcact ctcacgccag ggttcattcg gcgtttgaca
    13741 cgtaccggtt gccatcggtg attcgtctgc ttgagtatcg gcgcatcccg caccagacga
    13801 gggcgctctc gcgcaagaat attctgatgc gcgaccgcta cacgtgccag tactgcctgc
    13861 gcacgctccc gtcatccgaa ctgacgctgg accacgtgat cccccgctcc cgggctggcg
    13921 aaacaagctg ggagaacctg gttacctgct gtcacccttg caacaaccgg aaaggaaatc
    13981 gcaccccgga ggaatcggga ctgaagctcg tccggctccc aaggccgttc agtctgcaca
    14041 ccagccgaca cctcatgcgg cttctcggca agggtgacga ccagtggcgc aagtatcttt
    14101 tctattgaac ggcgggcggc ggctgggggc ccggcggcga tcctgacgag ggctgcggct
    14161 tgccttcctt cttcgcagcc gccttcacgg gactgatgat tacgtgggcg aagcgtcctt
    14221 ccagccgggg ctggccctcg accgcacccc actcggccag gtccttgacg aatcgcatta
    14281 gcaagttctt gcccaggtcg acgtgcgcga tctcgcgtcc ccggaactgg acaaccgcct
    14341 tcacgcgatt cccttctttc agaaagcgaa tggcgtggtt cttcttgaag tcgtagtcgt
    14401 gatcgtcggt attcggacga aacttgagtt cctttacctg gatcacgtgc tgcttctttt
    14461 tcgcttcctg ctgcttcttc ttttgctcat attgccattg gccatagtcc atggccttgg
    14521 cgacgggggg ttccgcggtc ggtgccacca cgattagatc gaggcccagt tcctgcgctc
    14581 gacgtagcgc ctgggacgtc ggcaagatct caacggaacc gtcgggaaat acggcacgca
    14641 cctcgcgagc acgaatcgcc tcattgacgt tttccctgat tctcgggcgg cgcgggataa
    14701 acggtctgcg tggaatcatc gatagtcttt gtcggaatgg gatctctgac atcccatgtt
    14761 caacctatca aagcacaaat tcagcccggt tgccagaggc aggttgcgca aaagcgagaa
    14821 gctgtggccc actgaagggt gatcgtgaga ccactgacgc ttcttcttgc ctgggcgtgt
    14881 ctgacagttt cggccgccga ggacggcgcg gcgatttaca agcaacactg tgccggttgc
    14941 cacgaaccgg agacggccgg acagacgccc gaccgcaacg cgcttgcccg catgtcttac
    15001 gacgccattc tgactacact cgacaccggc tcgatgaaat ccttgggggc cgatttgaca
    15061 ccagccgagc gtcgtgccgt ctcccggttc ctcgcacggc gcgcgtccgg cgggccgccc
    15121 cggacaatgc gcctggacta cttccacaca ggaaacgccc ggcaagagag tttcgccgtg
    15181 gacgggcttg tcttggaggg accgtggccg ggaaacccaa ggcgtgcgct cgacacgacc
    15241 ggcctcggca agtatttctt cgagatggtc gacggcgcct ctggccgggc gctgtactcg
    15301 cggggcttcg ctagcatatt cggcgagtgg gaattgacgc cggaggcaaa ggagaagcgg
    15361 gagacatttc acgagtcgct gcggttcccc gcgccgtcga agccggttcg cgtggtgcta
    15421 aaaaagcgtg gctcccgaaa cgagtggcgc gagatctggt ccgttccagt ggattcgtcc
    15481 ggaccctccg ccgaaagcgt gggtgtctca tcgagcgcgc gggtgtgggc cgtgacgaag
    15541 aacggcgaac cacaagacaa ggtggacgtc ctgttcctcg gtgacggcta taccgccacg
    15601 gatatggaga agtggcacgg cgacgcacgc cgcgtggcgg aactgctgtt tgccgagtct
    15661 ccattcaaag agcggaaacg cgatttcaat atctgggcaa tcgacacacc ggctggcgaa
    15721 agcggagttt cccggccctc cgacggtgtg cggcggtcgt ctcctatcgg cgcatcgtac
    15781 gacgcgttcg gttcggagcg atacgtcctt gtcttcgaca acaagcgcct ccgggaaatc
    15841 gcctcagccg caccgtatga gtttctcgcc atcatcgtga acgagcgcaa gtacggaggg
    15901 ggcgggatct tcaacctgta tgcaacctcg gcggcggata acgaattcac accctacgtc
    15961 ttcgtgcacg agttcggcca tcatctcgcc ggtctcgcag acgaatacta cacgtccgac
    16021 gttgcctacg agagcaccgc ggacaggccg gagccgtggg aaccgaatgt caccgccaac
    16081 ccgaaggcat cccggtggaa ggaactgctc acgccgggca tccccctgcc gaccccgtgg
    16141 tccagggacg agtttgagtc ctcccagaag aatgttcagg aaaagcgccg cgcgattcgg
    16201 tccaggaacc agcctgaatc tgaaatggaa gcgcttttcc gcgaggagcg cgggcacctc
    16261 acccgtttgc tcaaagccga acggtacgcc ggcaaggtcg gcgccttcga aggcgcgatg
    16321 tacgaaccac gcggatatta ccgcccgcaa gtggactgca tcatgttcac gagagacgaa
    16381 gtgggtttct gcgcggtctg caggcgggcc atcgaacgag tgatcgacct gtataccaga
    16441 ggccaataag agtgcccctg ctctgccgct gcctcattct tacgatcctc acggcggttg
    16501 tcggtcttac tgcgcagccg ggcgccaagc cgcgcctgct cgtcctgaca gacatcggcg
    16561 gagatccgga cgacacccaa tccctgatcc gtctgatgac gtactcgaac gagttcgaca
    16621 tcgagggcct cattgcgagc gcggctggaa cgcccggtga actcggcgtg gacatcgtga
    16681 agccggaact gatccgggag gttgtcgaag catacggccg ggtttgcggc acgctttcgc
    16741 tgcacgcggc cggttatcct ccagtggaac gcgtgctcga ttgcatcaag tccggcaatc
    16801 cgaagcgcgg cgtcggacaa ctcgggcacg acaaggacac cgaaggctcc cggtggatca
    16861 tcaaactcgc agaccggccc gacccacggc cgctgaacat cgccatctgg ggtggatcca
    16921 cggaactggc tcaagcgctc tggcgcgttc gcaacgactg cggccgcgac gggctgaacc
    16981 ggttcgtcgg ccggttgcga gtgcattcga tcgcacacca ggacgacacc ggcccctgga
    17041 tcatcgccaa ctttccggaa ctgttctatg ttctgagcat ggagcccgac ggcgtcgacc
    17101 ggcgccaggg tgcgtaccgc ggaatgtatc tcggcggcga cgaatcgctt acttcactgg
    17161 actgggtcaa cgcccacgtg aaaacggacc atggccctct cggagcgctc tatccgctga
    17221 agacttggac cgccccaaat ccgcatggtg ttctcaagga aggcgatacg ccatcctggt
    17281 tctacttcct gccgaacggc ctgggcgatc cggagcaccc cgaatggggc ggctggggag
    17341 gccgcttccg ccgtgcttcc ggacgcctct acagggacgc gcaggactcc actgcatctg
    17401 taactgacgc gcgcgccacc gtctggcgtt ggcgccccgc gtatcagaat gacttccagg
    17461 ctcgcatgga ctggtgtgtg aagggctacc gcgaagcgaa tcatcctccg ttggtcgtct
    17521 tggacagcaa acctggccgc gacgtcgtcc gaaagcagac gcgccctggc gcgaacgttc
    17581 cgctcaacgc cgccggctct tcggaccccg acggcgacca cgtctcctat cgctggtgga
    17641 tttaccgcga ggctggaagg ctctccgagc aggccgtcat cgagcgggct gaacgggagc
    17701 gggccaccgt gcggatccca agcggctcgg ccggagctga gattcacgta atcctcgaag
    17761 ccacggacag cggtgcgccc ccgctgatct cctatcgccg aatgatcctg gacgtgcgcc
    17821 cgtgacgcag ccgacgcgca gagcggccct atgtgttctg ctggccctga ccgctgcgac
    17881 gtttggtctg attttcgcgg tgagccacat gagccattcc gtgtggtatg acgagtctct
    17941 caccgacatc atcgcgaggc aaccaacgtt cggcgggctc gtcaagtccg ccatggattt
    18001 gaaagcgtac ccgccggcct tcttcgttgt ggtccgctgg gtgtattcga tcgatgacag
    18061 cccggccggt atgcggctgc cgtcggcggt ctttggcttt ctggcgacgc tcgccgtcct
    18121 cgctctcgga aggattgccg gcggtgggag cactcgccag cgccttcttc gttcttactc
    18181 ccggcgcgct ccgctatttc tccgacggaa acgcctacac actcctgggg tgaacccctg
    18241 ttgtgagaca attcgccgtt gctacacttg ccgttcaatg gtttttcctt ctcaaacagc
    18301 ctcttctcct ccagaatggt cctcggctgg cgaagcggag gcgggctctg gtggagacca
    18361 acccgccaag gactagtcag tcggagactc atcgagacga tgggtgaggg ggccggcttc
    18421 ggtcggctcc cgttgcgcac cggatcgata gatctcctca tgcctgaaaa aactcatcct
    18481 cgctcctgca gaacatacgt cagctgccac ctgctcgaac tcctccggtg agcgatagcc
    18541 cagcgccgag tgcaatcggt agcgattgta atactgttcg ataaattcct ctacgttggc
    18601 ccgcagatgc tcgaggtccc ggtactggtt tgcgtcaatt tcctctcgct tcagcgtctt
    18661 gataaagctc tcgcaactgg cattgtcata cgggttcgcc ggccgactca tgctgggaat
    18721 catctggtgc ttccgtaaga ttctcacgta atcaccggaa gcatactgga caccgcgatc
    18781 ggaatgatga accaaccctg gcagaggccg ccgttctgcc agcgcccgcg ccaaagccgc
    18841 aagtggcagc ctggctgcta acgttcgctc cagcgcccaa ccgaccactt tgcgtgaatg
    18901 accgtcaagg acgaccgcca agtaaacgaa ctcggctttc agtcgaatgt aggtgatgtc
    18961 ggccacccat agctggttga tcccggtcag cttcatgcgg ctcgccaggt tcaggaagac
    19021 ctccagcttg tgatccgaat cagtggtcac cacgaacgcc cgcggctgga tcgccagcag
    19081 gttatcctcc cgcatgatcc gcgccactcg cttgtggttc accagcattc cgcgccggcg
    19141 cagctcgatc gtgattcgcc ggtagccgta ccggcgacga tgctgaagcg caatctcctg
    19201 aatcgctgat cgtacttcca tatcctcttc cgccggcagc tgttctttca aagaccgata
    19261 gaaccctgcc cggctgacct gggccaattg gcacatccgc tcgacactca agctgccttg
    19321 cataggcatc aactctcgga cctgctcgta gacgccttcg cgccagagct gctgctgttc
    19381 tggcgtcgag cctcgacttt ttgcaaggca cctttgaaaa aatccacttc cagtactttc
    19441 tcgcccagca cgcgcttcag ctcgcggatc tcttttcgaa gttcgcgctc gcgggagttg
    19501 gcggctggcc ctggaccgtc ctcgaccggc tccatctgat cgcgccactt gtacagctgg
    19561 gttcgatgga tccccagctc
//
LOCUS       DATIFQ010000426         3639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_718162, whole genome shotgun sequence.
ACCESSION   DATIFQ010000426 DATIFQ010000000
VERSION     DATIFQ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718162"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VLE22_21305"
     CDS             complement(<1..1005)
                     /locus_tag="VLE22_21305"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSB17003.1"
                     /translation="MNQGIENIRKRGSRILTEGVHMLPAASLMMAEGAIRSLGDLKKP
                     FVTIVNSYTTQIPGHAHLDRLGAVLFEELKSLDFNVWYTNIGAAICDGIAMGHFGMKY
                     SLPSRELITDQIESIIGAHPCDAWIGIGNCDKIVPAMYNAMARINIPSVYVSGGPMLS
                     GRGNSDLVSVFEGVGRHSAGKMSEGELQRLAERACPGCGSCSGMFTANSMNCLGEVLG
                     LALPGNGTITAESWADREKGVTELNPERLGLVRRAAHALKRCLEDNVRPLDIITRRAL
                     DNAFVLDMAMGGSTNTVLHALALAHSAGIEYDLNRLNELSDVTPNICKVSPSRPDVHV
                     E"
     gene            1081..2949
                     /locus_tag="VLE22_21310"
     CDS             1081..2949
                     /locus_tag="VLE22_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17004.1"
                     /translation="MRLLTCVCAIVLLSSAAPAEMKVWAIGDTVRIDPIDGKAFEDNP
                     LMFPNCQTGDYRESNLIWDAKSKRITLRAARNEVVAFQIVVERRGSEKLSNARVEIGE
                     PAGPSGKKISLANIDLFKEWYVHVTQQSRQKYSLGTGYYPDGLLPCLRWTGNLYPHMY
                     VHPFEIPDMMNGIGDRQRNQALWVDIWVPRDRADAPPGTYASKIRITSNTGSEELTLQ
                     LQIWDFALPEQTHVMGNLHTDTELNVFPEDLELKYYQMIRRHRLAMGVLGYAPDLKVS
                     GTDVKIDWTKYDARLGKYLDGSAYTVQHGYSGPGEGVPTEILVLPFDAYPVNLYKNSI
                     GLRIGKEFKFYSPWPVAVPEGGPTPEYGEIWKKTFKLFDDHLNSNPAWNKTRPVVFLL
                     SLDEAYDDVARERMFYYGCLLKESGATRLEYRVDGWYPRETMLRLSEILRIAILGLGS
                     WEKDTVEEVRKRGVDPWFYTMAGLIDADPLVNRGLGWIAWKYKAGSWTLWELDFNSLR
                     AWIYPETYPDVRGEQNGHGMLIYRGETMGLTEPVASIRLKQLRRGSQDYEYFWLLAQK
                     AGGRGQADEAVNGIVSNFVECNCGVEPLGTPGMWKHNPDEWERARISLGELIDKTR"
     gene            complement(3005..3541)
                     /locus_tag="VLE22_21315"
     CDS             complement(3005..3541)
                     /locus_tag="VLE22_21315"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSB17005.1"
                     /translation="MMKGSSFRKLLVVAAVALIAAPLLAQGATGNGQLEQKVRHELLM
                     LPYYGVFDNLAFRVEGNKVVLLGQVSRPTLRPTAERVVKQIEGVSSVDNRIEVLPVSF
                     HDDRIRRGVLRAVYGNGALFRYNLGAIAPIRIVVKNGNVTLEGVVANEMDRNIAYLAA
                     NGVSGVFSVKNNLRVNNG"
BASE COUNT          750 a         1055 c         1152 g          682 t
ORIGIN      
        1 ttcgacatgc acgtcgggcc ggctgggaga gaccttgcag atgttcggcg tcacgtcgga
       61 cagctcgttc aaccggttga ggtcgtactc gatgccggcc gagtgcgcga gcgcgagggc
      121 gtggagaacc gtattcgtgg atccgcccat cgccatgtcg agcacgaagg cgttgtcaag
      181 cgcgcggcgg gtaatgatat cgagtgggcg aacgttgtct tcgagacacc gttttagtgc
      241 gtgagctgcg cgccggacca acccgagacg ctccggattc aactcggtga cgcctttctc
      301 gcggtcggcc cagctctctg cggtaatggt accgtttccg ggcaatgcca gaccgagaac
      361 ctcccctaga cagttcatcg aattggcggt gaacatgcct gagcagctcc cgcacccagg
      421 acaggcgcgt tccgcgagcc gctgaagctc gccctcgctc atcttcccgg cactatgacg
      481 gccgacacct tcgaagacgc tgaccaggtc gctgttgccg cgaccggaca gcatcgggcc
      541 gccgctcacg tacaccgacg ggatgttgat ccgggccatg gcgttgtaca tcgccggcac
      601 gatcttgtcg cagttgccga tcccgatcca tgcgtcgcac gggtgcgcgc cgataatcga
      661 ctcgatctgg tcggtgatca gctcgcgcga aggcagcgag tacttcatac cgaagtgacc
      721 catggcgatg ccgtcgcaga tagccgctcc gatgttcgtg taccagacgt tgaagtcgag
      781 cgatttcaat tcctcgaaga ggacggcgcc gagccggtcg aggtgcgcgt ggccgggaat
      841 ctgcgtcgtg taggaattga cgatggtgac gaacggcttt ttcaggtcgc cgagtgaccg
      901 gatggcgccc tcggccatca tcagcgacgc cgccggcagc atgtggacgc cctcggtgag
      961 gatgcggctg ccgcgtttgc gaatgttctc gatgccctgg ttcatgggag taagtaatca
     1021 gcttagcgcg gacaacacgg gtcgggtgaa ctataatcgt tcgcgtccat gggagggacg
     1081 atgaggcttc tgacatgcgt ttgtgccatc gtgctgctgt cgagcgcggc gccggcggag
     1141 atgaaggtct gggccattgg cgacaccgtg cggatcgatc cgatcgacgg gaaagcgttt
     1201 gaagacaacc cgctgatgtt tccgaactgc cagacgggag actacaggga gtcgaacctc
     1261 atctgggacg cgaagagcaa gcggatcacg ctcagggctg cgcggaacga ggtcgtcgct
     1321 ttccagatcg tggtggaacg gaggggctcg gagaaactct cgaatgcgcg cgtcgagatc
     1381 ggcgagccgg ccggtcccag cggcaagaag atctcgctcg cgaacatcga cctcttcaag
     1441 gaatggtacg tccacgtgac ccagcaatcg cggcagaagt attcactggg aaccggctac
     1501 taccccgacg gtctcctccc gtgcctgcga tggaccggga acctctaccc gcacatgtac
     1561 gtgcaccctt ttgagattcc ggacatgatg aacggcatcg gggaccgcca gcggaaccag
     1621 gcgctgtggg tggacatctg ggtgccccgt gatcgggcag acgcgcctcc gggaacctac
     1681 gcatcgaaga tccgaatcac ctccaatacg gggagcgagg aactcacgct tcagcttcag
     1741 atctgggact tcgcgctgcc ggagcagact cacgtcatgg gcaacctcca tacggatacg
     1801 gagctcaacg tcttccccga ggacctcgaa ttgaagtact accagatgat ccgccgccac
     1861 cggctggcga tgggcgtcct cggatacgct ccggacctca aagtgagcgg taccgacgtg
     1921 aagatcgact ggacgaaata cgacgctcgc ctcggcaagt acctcgacgg tagcgcgtac
     1981 acggtgcagc acggatatag cggcccaggc gagggagtgc cgacggagat actggtgctt
     2041 ccgttcgacg cctacccggt gaacctgtac aagaactcga tcggcctgcg gatcggtaag
     2101 gagttcaagt tctactcccc gtggccggtg gctgttccgg agggcgggcc gacgccggag
     2161 tatggcgaga tctggaagaa gacgttcaag ctgttcgacg atcatttgaa cagtaaccct
     2221 gcctggaaca agacccggcc ggtagtgttt ctgctgagcc tggatgaggc gtatgacgac
     2281 gtcgcccgcg aacgaatgtt ctactatggc tgcctgttga aggagtcggg agcgacgagg
     2341 ctcgagtacc gcgtggacgg ctggtatccg cgcgagacga tgctgcgcct gtcggagatc
     2401 cttcggatcg ccatcctggg cttgggctca tgggagaaag acaccgtcga ggaggtccgc
     2461 aagcggggcg tcgatccgtg gttctacacc atggcggggc tgattgacgc cgacccgctg
     2521 gtcaatcgcg ggctgggatg gattgcgtgg aagtacaagg ctggttcctg gacgctctgg
     2581 gagttggatt tcaattctct gcgtgcgtgg atctatccgg aaacttaccc ggatgtccgg
     2641 ggagaacaga atggccacgg aatgctgatc taccgcggcg agacgatggg actgaccgaa
     2701 cccgtggcca gcatccggtt gaagcagttg cggcgcggca gccaggacta cgaatatttt
     2761 tggctgctgg ctcaaaaggc agggggacgc gggcaggcgg acgaagcagt caatggcatc
     2821 gtgagcaact tcgtggaatg caactgcggc gtcgagccgt tgggaacgcc tggaatgtgg
     2881 aagcacaacc cggacgagtg ggagcgggcg cgcatcagcc ttggggaact gatcgacaaa
     2941 accaggtaaa aaaatggccc gacccaagga gggccaggcc gctcacttgc agagaatcaa
     3001 gaactcagcc gttgttgacg cgcaggttgt tcttcaccga gaacaccccc gagacgccat
     3061 tcgcggcgag gtatgcaatg ttccggtcca tctcattggc tacgacgcct tccagcgtga
     3121 cgttgccgtt cttcacaaca attcggatcg gtgcgatcgc tccgagattg tatcggaaga
     3181 gcgcgccgtt cccgtagaca gcacgaagga ctccccgccg gatccggtcg tcgtggaacg
     3241 acaccggcag aacctcaatc cggttatcga ccgaggacac tccctcgatc tgcttcacta
     3301 cgcgttcggc agtcggccga agtgtcggcc ggctcacctg tcccagcagg acgactttat
     3361 tgccctccac gcggaaggca agattgtcaa aaactccgta gtaggggagc atcagcaatt
     3421 cgtgacgaac cttctgctcc agttggccgt tgccggtcgc cccttgagcc agcagcggag
     3481 cggctatcaa cgccaccgcc gcgaccacaa gcaatttccg aaagctactg cctttcatca
     3541 tcccctcctt ctactcattg agatgcactg tgcgaagggg ccgttgcagg ggtgacagac
     3601 cttaacagaa actttacaga aatacgccca agctattga
//
LOCUS       DATIFQ010000427         3451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1100798, whole genome shotgun sequence.
ACCESSION   DATIFQ010000427 DATIFQ010000000
VERSION     DATIFQ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100798"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VLE22_21320"
     CDS             <1..514
                     /locus_tag="VLE22_21320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17006.1"
                     /translation="VAFAEVSRRLKRTGAVLLAAVLVLACGLSLLVSNEYLSQLIRNG
                     GARSWTDASQPLCETLERTPAELVYIVDWGIFDTQRLLSEGRLPLRWGVTPLVKEKLD
                     DTDWREFREMISDPQHIVVGHTPAYEEFPNVRERLRVLSEQEGYRPEILHTVRDSNGR
                     AVYEVFRFRQ"
     gene            complement(508..1548)
                     /gene="cydB"
                     /locus_tag="VLE22_21325"
     CDS             complement(508..1548)
                     /gene="cydB"
                     /locus_tag="VLE22_21325"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HSB17007.1"
                     /translation="MEILWFCLVAIMLAAYVVFDGFDLGAGIIHFCVARTEDEKRKVL
                     RSIGPVWDGNEVWLLAAGGTLYFAFPRLYASSFSGFYLPLMMVLWLLILRGVSIEFRN
                     HVESPVWKPIWDAGFMVSSALLAVFFGAALGNVVRGVPLNPAGEFFLPLWTHFGVTGE
                     VGILDWYTVLVGVFAFLTLTQHGALWVAMKTDEDVHRRARRIASVSWFGVLLLTVLVT
                     ILTMQVQPKVWANLTSRPWGFVFPAVAVAGLIEVYVCSRSGNDVKAFLASCAFIVGML
                     TSAAFGVFPCVMPSNIDPSQSLTIYNSATAAYGMKVGLAWWIPGMILVASYFVHTYRN
                     FAGKVSLEGEGY"
     gene            complement(1558..2907)
                     /locus_tag="VLE22_21330"
     CDS             complement(1558..2907)
                     /locus_tag="VLE22_21330"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSB17008.1"
                     /translation="MDSALALHRLHFAFTVTYHYLFPQLTMGLALLIVILKTKALRTG
                     NEHYNRAARFWAKIFAISFAMGVVTGIPMEFQFGTNWARFSRAAGGVIGQTLAMEGTF
                     AFFLESSFLGLFLFGEKRLGPKGHWWAAFLVFLGSWLSGFFIIATDAWMQHPTGYRIG
                     PNGEIVLDSFWDLLLNPWTLWQYPHNMIGAVVTGCFVMASVGAFYLLSHKHEEYGRTF
                     VRLGVTVGFIASVLMLFPTGDGQGKNIAKYQPATLAAMEGLFETTEGAPIALIGQPDM
                     EKRKLDNPLLVPRALSFITYQRWKAEVKGLDAYPPSEWPDNVPLLYYSYHIMVGLGTI
                     FIAVTAICFWKLRKGSLFALRPLLWILAASLPFPFISNTAGWITAELGRQPWVIYGLM
                     RTSDGASMNVSAGNALFTLIGFMGVYTVLSILFLFLMHREIDHGPEHGAVPAAGVTH"
BASE COUNT          744 a         1047 c         1059 g          601 t
ORIGIN      
        1 cgtcgccttc gcagaggtgt cccgccgttt gaaacgaact ggtgcggtcc tgctggcggc
       61 cgtgctggtg ctcgcctgtg gattgagcct gctggtctcc aacgagtacc tttcgcaact
      121 gatccgcaac ggcggtgcgc gctcgtggac tgatgcgagc cagccgctat gcgaaacact
      181 cgagcgaaca ccggcggagc tcgtctacat cgtcgactgg ggtattttcg acacgcagcg
      241 cttgctcagc gaagggcgct tgcccctgcg ctggggcgtt acaccactgg tgaaagagaa
      301 actcgacgat acagactggc gcgagttccg cgaaatgatc agcgatcccc agcacatcgt
      361 cgtcggccac acaccggcat atgaggagtt cccaaacgtg cgcgaaaggc tgcgcgtcct
      421 atccgaacaa gagggctacc gcccggaaat cctccacacc gttcgggaca gcaacggccg
      481 agcggtctac gaggttttcc gattccgtca gtagccttcc ccttcgagac tcactttgcc
      541 ggcaaagtta cggtaggtgt gcacgaagta actggccacc aggatcattc caggaatcca
      601 ccaagccagg ccaaccttca ttccgtacgc ggccgtggcg gagttgtaga ttgtcaggct
      661 ctgactcgga tcgatgttcg acggcatcac gcagggaaag acgccgaagg ccgcgctggt
      721 cagcatgccg acgatgaagg cgcaggaagc caggaacgct ttgacgtcgt tcccggaccg
      781 gctgcacaca tacacttcga tcagcccggc gaccgcaacg gctgggaaaa cgaatcccca
      841 cggccgcgac gtcaaattgg cccagacttt cggttgtacc tgcatcgtca ggatggtcac
      901 caggaccgtc aggagcagca caccgaacca tgaaacggaa gcgatgcggc gggcgcgccg
      961 gtgaacgtct tcatccgttt tcatcgccac ccaaagcgcg ccgtgctggg tgagcgtcag
     1021 gaacgcgaaa acgccgacca gcaccgtgta ccagtcgaga atgccgacct cgccggtcac
     1081 gccgaagtgc gtccataggg gtaggaagaa ctcgccggcg ggattcaacg gcacgccgcg
     1141 gacaacgttc ccaagcgccg cgccgaagaa gacggccagc agcgcgctcg agaccatgaa
     1201 tccggcgtcc cagattggtt tccacaccgg gctttccaca tggttgcgaa actcgatcga
     1261 gacgccccgg agaatcagca gccagagcac catcatcaat ggcagataga aaccgctgaa
     1321 gctcgacgca tacaggcgcg gaaaagcgaa gtacagcgta cccccggcgg cgagaagcca
     1381 tacttcattg ccgtcccaca ccgggccgat cgagcgcagg actttgcgct tttcatcttc
     1441 ggtacgggcc acgcagaaat gaatgattcc cgccccgagg tcaaagccgt cgaacacaac
     1501 ataggcggcg agcatgattg cgaccagaca gaaccagagg atttccatgg gtgcctccta
     1561 atgtgtaaca ccggccgcgg gcaccgcgcc gtgctccggt ccgtgatcga tctcacggtg
     1621 catcaggaac agaaacagaa tactgagcac ggtatacacg cccatgaagc cgatcagcgt
     1681 gaacagcgcg ttaccagccg agacgttcat cgacgcaccg tcgctcgtgc gcatcagacc
     1741 gtagatcacc cacggctgac ggccgagctc ggcggtgatc catccggcgg tgttggagat
     1801 gaaggggaaa ggcagcgacg ccgccaggat ccatagcaag ggccgcaacg cgaacagtga
     1861 gccctttcgc agtttccaga agcagatggc cgtaaccgcg atgaaaatgg tcccgagccc
     1921 gaccatgatg tggtagctgt aatacagcag tggaacgtta tccggccact cggacggcgg
     1981 gtacgcatcc agtcctttca cctcggcttt ccaccgctgg taggtgatga agctcagcgc
     2041 ccgggggacc agcagcgggt tgtctagctt ccgtttctcc atgtccggct ggccgatgag
     2101 cgcgatcggc gcgccttcgg ttgtctcgaa gagcccctcc atcgcggcca gcgtggccgg
     2161 ctggtacttc gcgatgttct ttccctggcc gtcgccggtc gggaagagca tcagcacaga
     2221 ggcaatgaag cccaccgtga cgcccaaccg aacgaacgtg cgcccgtact cctcatgctt
     2281 gtggctgagc aggtagaaag cgccgacgga ggccatcacg aagcagccgg tcaccaccgc
     2341 gccgatcatg ttgtgcgggt actgccatag ggtccacggg ttgaggagca ggtcccagaa
     2401 actgtcgaga acaatctctc cgttcggccc gatcctgtag ccggtcgggt gctgcatcca
     2461 ggcgtcggtg gcgatgatga agaagcccga aagccacgac ccaagaaaga ccaggaacgc
     2521 cgcccaccag tggcccttcg gcccgagccg cttctcgccg aacagaaaca ggccgaggaa
     2581 gcttgattcg aggaagaagg caaacgttcc ttccatggcc agcgtctgtc cgatcacccc
     2641 gccggcggct ctggagaacc gggcccagtt cgtgccgaac tgaaattcca tcgggatgcc
     2701 ggtgaccacc cccatcgcga aggagatggc gaaaatcttc gcccaaaatc gggcggcacg
     2761 gttgtaatgc tcgttgcccg tgcggagcgc tttcgttttc aggatgacaa tgagcagcgc
     2821 cagccccatg gtgagctggg ggaagagata gtgatacgtg accgtgaatg cgaagtgtaa
     2881 tcgatgcagt gccagcgcgg agtccataga caaggcctct tccgaaataa agctgcaact
     2941 tccagtgcag gataacagag cgggaactcc ggttgtagcg tcaggtccgg ccggtccggc
     3001 ctatgtccgg gaaaccggaa gagcgcggtt cgtcccggca tcaaaggcgc cgctttcggc
     3061 gggtgacgga cagaacttag aatggtcagg aatccatgct cgatgtagtc attcatcatt
     3121 cgccatcgga tcgagaagtg gccaccgcga tcgccgaccg cctgcagcgc ggggcggaag
     3181 ccaaggtatg gctagaggag tgccgcttgg gggatgggca gagtattgcg tcggtgtggg
     3241 agaccgctga ctccagcggc ggggctgtcc tattgctctc ccggaatttg gtcccggttc
     3301 ggttgaaccg ccggcactgg gagccactgt tgactcgggt ggagagaaac gcggcgactc
     3361 gtctcggtgc tgtccttctc ggcgattgcc cttacccgcg tcttcttgag cgcacacact
     3421 tctttcgttg gatggatggc acgcgagagg c
//
LOCUS       DATIFQ010000428         6721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_718837, whole genome shotgun sequence.
ACCESSION   DATIFQ010000428 DATIFQ010000000
VERSION     DATIFQ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718837"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..577
                     /locus_tag="VLE22_21335"
     CDS             122..577
                     /locus_tag="VLE22_21335"
                     /inference="COORDINATES: protein motif:HMM:NF013151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17009.1"
                     /translation="MSTGPHRQLRKDGVRKLGKALGLPAETYQRMPFPGPALAARVVG
                     EATPEKLELVRKATAIVEGELKDVKKFQCMAILHEDMFPGMKAGKREYGMQIEIRCWT
                     SVDARTATPVWLPSKVLERMAARITRGIPGVVSVTYNIAPKPPSTMEAV"
     gene            complement(648..1322)
                     /locus_tag="VLE22_21340"
     CDS             complement(648..1322)
                     /locus_tag="VLE22_21340"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HSB17010.1"
                     /translation="MKLRILIALSLGTGVLSAQPDHVKRLDAAATVFSEVMATPDKAV
                     PQELLDKAECIVVVPGLKKGAFIFGAKYGKGFFSCRKKGGVGWTGPAAVRIEGGSFGL
                     QIGGSETDVIMLVMNEHGAKRLLDSKFTLGGDASVAAGPVGRTATAQTDAYMTAEILS
                     WSRSRGVFAGISLQGATLRQDLDDNQALYGRKLENKEIITQQMAPPKAAEKLISAFNK
                     YSSRKG"
     gene            complement(1323..3770)
                     /locus_tag="VLE22_21345"
     CDS             complement(1323..3770)
                     /locus_tag="VLE22_21345"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB17011.1"
                     /translation="MLLLTVLTAALQAQVRVNGRVVDENGAVVPGARVEFRQPGLVQA
                     PFIVSDSQGRFEIGLPVPGQYALHAERPGFFVVERASTTLNEGVNEVTITLNHLREFV
                     ETVDVVYSAPVIDPADTTEQKQLNSMEILEVPYPASQDVRNALPLFSGVVQDNGGQLH
                     FNGGASEQTNFNLDGFNISDPVTGLFEARLSIDSVRSLDLESARFAADKGRGSAGSLD
                     IKTGMGDERWRFGATNFIPGVSTERGLLLNKWTPRLTVSGPIARGRAWFQNGFDAFYD
                     LDIVKELPPGEDQTRSFTTSNLSRFQVNLTPANILTGSFLMNYGDDDHHGLSFLDPQE
                     TTIHRRRTLYMATVKEQIYFQRGALVEFGFALSRGSTRETPLGSQTYEISPSGRRGNY
                     FVDLDRDTGREQWIVNAFLPPWKAAGQHQLKVGADLQRSGFDQKTERHDYVVLRTDGS
                     IAREVSFTGNGLLSKKNFESTLYVQDRWTPREGLLVEAGLRTDWDQIVRDLLFSPRLA
                     VAWSPGWLRETKLAAGFGVFSDSLSLATLTRHQDQTSVALFFSRSGELMRGPIETGFV
                     VDEQSLKVPRARIFSVSIERKLPCQFYGKAGYLRRSGMDGFTFVEPRMNVNPETVFYT
                     LRNQRSDRYNALDFSVRRTFGGKFEWVSGYTYSRARSNAVVEYSLENPIFAPQAPGPV
                     EWDTPHRFMMWGWAPLPRAILPPVLNWLLKDTNASYLAEARSGFPFSVVNEEGFSVGK
                     PNERRLPYYFNINLHLERRFQFLNYLWAWRFGFNNLTNHGNPNVVNNNIDSPFFLNYG
                     RGQHRAFSVRLRFLGRR"
     gene            3865..4428
                     /locus_tag="VLE22_21350"
     CDS             3865..4428
                     /locus_tag="VLE22_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17012.1"
                     /translation="MWRMLTALALFVITLPAVTGGDYRSAKRKIDQIQADRAPSGTRI
                     TLTTNELNAWVRAEVPPSVRGAMRSPTLELGHGSATGSAWIDFVKLRTAQGSPPGWLL
                     RTLLDGEHPVRVTANVRSGAGRAVVDVQEVEVSGFEISGRALDYMVRNFLLPRYPNAK
                     IGEPFEMGHRIERLDIAPAGVGVVIGK"
     gene            complement(4415..5788)
                     /gene="hemG"
                     /locus_tag="VLE22_21355"
     CDS             complement(4415..5788)
                     /gene="hemG"
                     /locus_tag="VLE22_21355"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HSB17013.1"
                     /translation="MRAVVIIGGGVSGLSTAYHLSKAGIPSTVVERRPYLGGVVRTDS
                     AHGCLLEAGPDSFLAAKPWALELIRELGLAGDVIGSNDELRVTYIVKDGKLLPMPDGL
                     MLMVPTRILPMVSTRLLGWPTKFRMGWEVFRRPGNAPSEDRSVADFIRDHYGEEAVDY
                     LAEPLLAGVYGGDPEKLSVASVLNRFVELEEKYGSLSRGVLAERRKMRRKEGNPPLFQ
                     TLRGGLGKLINTLVSAAGHSVNVIEADALAVERQADGYRVMLSDGSLDARHVVLACQA
                     HEAGGVIRPLDDPLADLLAAIPYNSSVTISLGFAKSTFDCRLNGFGFLVPKRERKRVM
                     AGTWVGTKFAHRVPEDKVVLRCFLGGDSSASLQESDDALVETVREELREILGLHSQPM
                     FWRVARWPRSMAQYVVGHQKRVEEIEARLSGAPGVHLVGNAYYGIGVPDCVRMGKQVA
                     ERIATSR"
     gene            complement(5790..>6721)
                     /gene="hemH"
                     /locus_tag="VLE22_21360"
     CDS             complement(5790..>6721)
                     /gene="hemH"
                     /locus_tag="VLE22_21360"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HSB17014.1"
                     /translation="GCRADGSRIPPMKQAVLLLAHGAPERLEDVEPYLSLVRGGRALA
                     PHVVEEIRQRYALIGGSSPLLARTREQAEALERKLAEEGFPVRVYLGMRNWRPFIREA
                     MAEIRTDGVDRIVAICLAPQYSRLSVGLYFRRTQEAKQELGVRAEIAWTKSFHSHPLL
                     VEAFAERLKPLLPCPTVLFTAHSLPERILEKGDPYDTEARATAKAVAERCGLADWDFA
                     YQSQGMTEESWLGPTVESRIDQYAREGIRDVVLAPIGFVCDHVEILYDVDVLFRSYAT
                     ERGVRLQRPESLNASTKFIATLATVARERLLTS"
BASE COUNT         1314 a         2081 c         2001 g         1325 t
ORIGIN      
        1 acgatctcgc cgggctcgag atcccgaacc cgccggaagc cgaggttggc aaaaccgccg
       61 cattcggacg caaccacgac ggctccctgt ttctctccaa tgaccagcgg ccagcggccg
      121 gttgagcacc ggaccgcata ggcaactgcg caaggacggc gtcagaaagc tcggcaaggc
      181 gctcgggctg ccggcagaga cgtaccagcg gatgcccttt ccggggccgg ccctggctgc
      241 aagggtcgtt ggcgaggcta cgccggagaa acttgagctt gtccgaaagg cgaccgcgat
      301 cgttgaaggg gaactcaaag acgtcaagaa gttccagtgc atggcgatcc ttcacgagga
      361 catgtttccc ggcatgaagg caggcaagag agagtacgga atgcaaattg agatccgctg
      421 ctggacgagc gttgacgccc ggacggctac tccggtctgg ctgccttcca aagtgctgga
      481 gaggatggca gccagaatca caaggggaat ccccggcgtg gtgagcgtca cctacaacat
      541 cgccccaaaa ccgccgtcca ccatggaagc ggtttgacgc cgctgggcgc tggcagcagg
      601 gtggctggca tgaaccggcg cccggagctt ctattgtcta tccgccttca acctttgcgg
      661 cttgagtact tgttgaacgc cgaaatcagc ttctcggcgg ccttgggcgg cgccatctgc
      721 tgggtgatga tctctttgtt ttcgagcttg cgtccgtaaa gggcttggtt gtcatcaagg
      781 tcctggcgca gcgtggctcc ctgtagtgaa atcccagcga acacaccgcg agaacgcgac
      841 cacgacagaa tttcagcggt catgtaggcg tcggtttgtg cggttgctgt tctcccgacc
      901 ggtccagcgg ctaccgacgc atcgccgccg agagtgaact tgctgtcaag gagccgcttc
      961 gcgccgtgct cattcatcac gagcatgatc acatcagtct cggagccccc gatctgtaac
     1021 ccgaagcttc caccttcgat ccggacggcc gccggccctg tccagccgac tccgcccttc
     1081 ttgcggcatg agaagaatcc cttgccgtac ttggcgccga aaataaaggc gcccttcttc
     1141 aaaccgggta ctaccacgat gcactcggcc ttgtccagta gttcttgcgg taccgccttg
     1201 tccggtgtcg ccatcacctc cgagaaaacc gtggcggcgg catcaaggcg ctttacgtgg
     1261 tctggttgcg ccgaaagcac gcccgtgccg agtgacaggg cgataagaat tctgagtttc
     1321 atttatctcc tccccaagaa cctcagccgt acgctgaagg ctctatgctg gcccctcccg
     1381 tagttcagga agaagggcga gtctatattg ttgtttacca cgtttggatt cccgtggttg
     1441 gtcagattgt tgaagccaaa gcgccacgcc cacaagtagt tcaggaactg gaaccgtcgt
     1501 tccaaatgca ggttgatgtt gaagtagtag ggaagccggc gctcgttcgg ttttcctacg
     1561 gaaaaaccct cctcgttgac gacactaaat gggaacccgc tcctggcttc ggcgagatac
     1621 gacgcgttgg tgtccttcag cagccagttc aggacggggg gcaggatcgc gcgagggaga
     1681 ggcgcccacc cccacatcat gaatctgtgc ggcgtatccc actcaaccgg cccgggagcc
     1741 tgcggagcaa agatcggatt ctcgaggctg tactcgacaa ccgcattgga cctcgctctg
     1801 gagtacgtgt atccggacac ccattcgaat ttgccgccga aggtgcggcg cacggagaaa
     1861 tccagtgcgt tgtagcggtc gctgcgttgg ttgcgtagcg tgtagaacac cgtttcgggg
     1921 ttgacgttca tcctcggctc aacgaacgtg aatccgtcca ttcccgagcg tctgagataa
     1981 cccgcctttc cgtagaactg gcatggaagc ttgcgctcga tgctcacgct gaagatccgc
     2041 gcccgcggaa ctttcagtga ctgctcgtcc accacgaaac ccgtttcaat gggtcctcgc
     2101 atcaattcac cggatcgcga aaaaaaaagg gccacactgg tctggtcctg atgccgtgtc
     2161 agcgtagcta gacttagtga gtcgctgaac acgccaaatc cagcggcgag tttcgtttcc
     2221 ctcagccatc ccggagacca ggcgacagcc agtcgaggcg aaaagagcag gtcgcgcacg
     2281 atctggtccc aatccgtccg taggcctgcc tcgaccaaga gtccttcccg cggagtccag
     2341 cggtcctgga cataaagcgt ggattcgaag ttcttcttgg acaacagtcc gttgcctgtg
     2401 aaggaaactt cccgcgcgat tgaaccgtcc gtacgcaaca cgacatagtc gtgccgttcg
     2461 gttttctggt cgaagccgct acgctgcaag tcagctccca ccttcagttg gtgctggccg
     2521 gcggctttcc acggcggcag gaaggcgttg acgatccact gctcacggcc cgtgtcccgg
     2581 tccaggtcga cgaagtagtt accgcggcgg ccggacgggg aaatttcata cgtctgggaa
     2641 cccaatgggg tctcccgcgt cgagccgcgg ctcaaggcga aaccgaattc gacgagcgcg
     2701 ccacgttgga agtagatctg ctccttgacc gtggccatgt agagagtccg ccggcggtgg
     2761 atggtcgtct cctgcggatc gagaaacgac aggccgtgat ggtcgtcgtc tccgtagttc
     2821 atcaggaagc tacccgtgag aatgttggcc ggtgtgaggt tcacctgaaa gcggctcagg
     2881 ttgctcgtcg tgaaacttcg ggtctggtct tcgcccggcg gcagttcctt gacgatgtca
     2941 agatcataga acgcatcaaa gccgttctga aaccaggccc tgccacgcgc gatcggtccg
     3001 gaaacggtaa ggcgcggcgt ccatttgttg aggagcagcc cgcgttccgt cgacacgccc
     3061 gggataaaat tcgtcgcgcc aaatcgccac cgttcgtctc ccattcccgt tttgatgtcg
     3121 agggagccag cgctcccacg gcccttgtca gccgcaaacc gtgcgctttc cagatccaga
     3181 ctgcgcaccg aatcgatgct gaggcgagcc tcaaacagtc cagtcacggg gtcagagata
     3241 ttgaagccat ccaggttgaa gttcgtctgt tcgcttgcgc ctccgttgaa atgaagctgc
     3301 ccgccgttgt cctgcaccac acccgagaag agtgggagtg cgtttcggac atcctgggaa
     3361 gcgggataag gcacctccag gatctccatg ctgttgagtt gcttctgttc tgtcgtgtcg
     3421 gcagggtcga tcactggcgc ggagtagacg acatcgaccg tctcgacgaa ctccctcaaa
     3481 tggttcaacg taatggtgac ttcgttgaca ccttcgttga gagtcgttga ggcccgctcg
     3541 acgacgaaaa aaccgggccg ttcggcatga agggcgtact ggcccggtac cggcaacccg
     3601 atctcaaacc ggccctggct gtctgagacg atgaacggtg cctggacgag accaggctgc
     3661 cggaattcca ctcgtgctcc gggcacgacc gctccatttt cgtcgaccac cctgccgttc
     3721 accctcactt gagcctggag agcagccgtc aagacggtca gcaggagcac cgcccgcaga
     3781 cagccacttc tccgggcagg agcagcccgt atgctttcat tgtaatgccc ggagtctgcc
     3841 gcgcctgatc ggtacaatgt aggtatgtgg cgcatgttga ccgcgcttgc gctgttcgtc
     3901 atcactctcc cagccgtgac cggtggcgat tatcggagtg cgaagcgcaa gatcgaccag
     3961 atccaggctg accgggcgcc ctccggaacc cggatcacgt tgactacaaa tgagttgaat
     4021 gcctgggttc gagccgaagt gccaccttcg gtccgggggg ccatgcggag tccgacgctt
     4081 gaactgggcc atggttccgc aacgggctct gcctggatcg acttcgtgaa gttgcgaacg
     4141 gcgcagggtt cgccgccagg ctggctcctc cggacgctgc ttgacggcga gcacccggtt
     4201 cgggtgacgg caaacgtccg ctccggtgcg ggacgagcag tcgttgatgt tcaggaagtc
     4261 gaggtttcgg gcttcgaaat cagtggaagg gcgcttgact acatggttcg gaatttcctg
     4321 ctcccgcgct acccgaatgc aaagatcggt gaaccgttcg agatggggca ccgtattgag
     4381 cggctggaca ttgccccggc tggcgtcgga gtggttatcg ggaagtagcg atcctttccg
     4441 ctacctgctt tcccattcgc acacagtcag gaaccccaat gccgtagtat gcgttgccga
     4501 caagatgcac gccgggcgca ccggagaggc gggcttcgat ttcctccact cgtttctgat
     4561 gaccgacgac atactgcgcc atcgagcggg gccatcgggc cactcgccag aacattggtt
     4621 gggagtgcaa tcccaggatc tcgcgcaact cctcgcgcac agtttccacc agtgcgtcgt
     4681 cgctctcctg gagggatgcg ctgctatcgc cgccgaggaa acacctgagc accaccttat
     4741 cttcgggcac gcgatgggca aatttcgttc cgacccatgt gcctgccatg acgcgcttcc
     4801 gctcgcgttt cggcaccagg aatccgaacc cgttcaggcg acagtcaaac gtgcttttcg
     4861 cgaaccccag ggaaatggtg accgacgagt tataggggat tgccgcaagt aggtctgcca
     4921 gcggatcgtc gagcggccgg atcaccccgc cggcttcatg cgcctggcaa gcaagaacga
     4981 cgtgtctggc gtccagtgag ccgtctgaaa gcatgacgcg gtagccgtcg gcttgccgct
     5041 cgacagcgag cgcatcggcc tcgatcacat tgacggaatg ccctgccgcc gaaaccagag
     5101 tgttgatcag cttgcccaac ccgccgcgaa gcgtctgaaa caaaggagga ttcccctcct
     5161 tccgccgcat cttgcggcgt tccgccagca cccctcgcga cagacttccg tacttttcct
     5221 cgagttctac gaaacggttc aatacgctcg ctacgcttag cttctcggga tcgccgccgt
     5281 agactccggc aagtaacggt tcggccaaat agtcgaccgc ttcctcgccg tagtggtctc
     5341 ggatgaaatc cgctaccgac ctgtcctcgg atggcgcgtt tcccggtcgc cggaacacct
     5401 cccagcccat gcggaacttg gtaggccagc cgaggagccg tgtcgagacc atcggcaaga
     5461 tgcgagtcgg caccatcaac atgagcccgt cgggcatcgg aagaagcttc ccgtccttga
     5521 cgatataggt gacgcgcaac tcgtcgttcg agccgatcac atcgccggca agacccagct
     5581 cgcgaatgag ctcaagcgcc catggcttgg cggcgagaaa actgtcgggg cccgcttcaa
     5641 gtaggcaacc atgcgccgaa tccgttcgga ccaccccgcc gaggtacggg cgccgttcca
     5701 caaccgtgga gggtatgccg gccttagaga ggtgatacgc ggtggacaat cccgagacgc
     5761 ccccgccgat gattactacg gcccgcatct caggacgtga ggagtctctc cctggccacg
     5821 gtagccagtg tggcgatgaa cttagttgag gcgttgaggg actcgggtcg ttggagccgg
     5881 acgccacgct cggttgcgta gctccggaag agtacgtcga catcgtacag gatctctaca
     5941 tgatcgcaaa cgaaaccaat cggcgcgagt acgacatccc ggatgccctc ccgggcgtac
     6001 tggtcgattc gagactccac ggtcgggccg agccagctct cctcggtcat gccctggctc
     6061 tgataggcga agtcccagtc cgccaacccg cagcgttcag ctaccgcctt cgccgtggcc
     6121 ctcgcctcgg tgtcgtaagg atcgcccttt tcgaggattc gctccggcag cgagtgtgcg
     6181 gtgaagagga ccgtcgggca ggggagcaac ggcttgagcc gctctgcaaa cgcctccaca
     6241 agaagcggat gtgaatggaa gctcttcgtc caggcgatct ccgcacggac tcccagttcc
     6301 tgctttgcct cctgcgtcct ccggaagtac agcccgacgc tcaagcggga atactgtgga
     6361 gccaggcaga tggcgacgat gcggtccacg ccgtccgtcc tgatctctgc catggcttcc
     6421 cggatgaacg ggcgccagtt gcgcatcccc aggtaaaccc tgaccggaaa gccttcctcc
     6481 gcgagcttgc gctcgagcgc ctctgcctgc tctcgcgtgc gggccagcag cggtgaactc
     6541 cccccgatca gagcgtatcg ctgccggatt tcctcaacga cgtgcggtgc gagcgccctg
     6601 ccgccccgga ccagactgag gtacggttca acgtcttcca gccgttccgg cgcgccgtgt
     6661 gccagaagca gaacagcctg cttcatgggc ggaattcgcg aaccatctgc acgacagcct
     6721 t
//
LOCUS       DATIFQ010000429         4128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2249100, whole genome shotgun sequence.
ACCESSION   DATIFQ010000429 DATIFQ010000000
VERSION     DATIFQ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2249100"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VLE22_21365"
     CDS             complement(<1..192)
                     /locus_tag="VLE22_21365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17015.1"
                     /translation="MSNSNQRRLNRILVPLFFALEVAALAGLASPSAIAADVIGFHKV
                     VLDAQGKLLPAIGPAEVAYD"
     gene            complement(225..1766)
                     /locus_tag="VLE22_21370"
     CDS             complement(225..1766)
                     /locus_tag="VLE22_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17016.1"
                     /translation="MNFRILALALALAGLAATPAVAAEVLIYHDIQTDASGRIIPWAG
                     PPAKAYDHIVQLVWNFWIKMRQCPNGVPYYMQHQVWKPDQDDKRGLGGDQINMALSSW
                     TLLYGYLGDPAVRQNMIYMADYWLDHGMSKPSDVWANFPYPYNTELHSGQYDGDMMVG
                     KGYLQPDKAASFGAELVVLWKMTQNQRYLDAAVKIANTLVRHVKPGDNDNSPWPYRVH
                     AVTGEVRQVKSDGGGVYLASYTTNWTGALRLFEDLAALGKGDVDTYRRTRQTLVAWLK
                     TYPLKTNKWGPFFEDVGRWSDTEINADTLAWYILEHPEWDSNWREQAKSILGWSFKMF
                     ANREWEKYGVVAINEQTAYKVPGNSHTSRYASVELLYAEKTGDLARKEDAIRQLNWAT
                     YMVDDDGKNRYPRNDNWLTDGYGDYVRHYLRAMAAFPELAPDDQNHLLRTSSVIRTLA
                     YGPDRISYEKSDASSAERLKLGAWAPGRVSGGEMQWDPKTKVLTVSSNKNQVTIWRAK
                     SGSKI"
     gene            1960..3231
                     /locus_tag="VLE22_21375"
     CDS             1960..3231
                     /locus_tag="VLE22_21375"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17017.1"
                     /translation="MSAISDCQRAGNRLTRRQIFSGVAYCSASAALSARPRADELYQK
                     KSSQVIRMGIVGGNFGASFFWHEHPNSRVTAVCDLRDDRLERLQKVYRCETGYKNYHE
                     MVADKNVDAIGVFTPWNLHVDQAIEAMKAGKHIISAVPAGISIESCERLLYWVQKTGM
                     TYMMAETSYYRPEIMACRRWAKEGQFGTIFYAEGEYYHDGIENLWYDERGEPTWRRAR
                     PPLWYITHATGCVIPVMGERLTEVVAHGYGDMEHPGVLGDGLRTNFYSNPFWNGIAMF
                     KTSAGHAVRINVFRHVAAREAERAQFFGTEMSYYMAVPGLHPNSRNVREKTGKIRTID
                     NYPVTAIRSEPFEPANFHEMLPPELRKPSGHGGSHTFLTHEFVSACIERRRTEVDIYE
                     ALAYTVPGQYAHRSALEGGTLKKIKNYERPA"
     gene            3454..>4128
                     /locus_tag="VLE22_21380"
     CDS             3454..>4128
                     /locus_tag="VLE22_21380"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB17018.1"
                     /translation="MYKRSIVSAALAVVFSFAFLGSPSFAQINTGRVTGTVLDATGAA
                     VPAVSIRAVNEDTGVTTSTESLATGDYLLNFLVPGRYRVEAEKTGFQKSLQTGIAINA
                     GGITHIDIRMQVGEVRQTVEVAGNPIAVSTETSELSQTFSKTDLDKLPNIDRNPLFQM
                     NLMPGANNDRGSGNYGNNGGENGSAIGNQRNQLASIGGVNANANSVFIEGTFNREPQN
                     AYVGVVP"
BASE COUNT          812 a         1242 c         1270 g          804 t
ORIGIN      
        1 gtcgtacgct acttcggccg gcccgatcgc ggggagcagc ttgccctgag cgtccagaac
       61 aactttatga aaaccgatga cgtccgcggc gatggccgac ggcgaagcaa gtccggccag
      121 ggccgccact tctagcgcga aaaatagggg caccaggatg cgattcaatc gacgctggtt
      181 gctgtttgac ataacgatga ttgtgcgagc cccagaactc ttagctagat tttcgacccc
      241 gatttcgcgc gccagatcgt cacctggttt ttgtttgacg acacggtcag gaccttcgtt
      301 ttcggatccc actgcatctc gccgccgctg actctcccgg gcgcccaggc gcccagcttg
      361 aggcgctcgg ctgagctggc atcagacttt tcgtaactaa tcctatccgg gccgtaggcc
      421 aaggtccgga tcaccgagga cgtgcgcagc aagtggttct ggtcgtccgg cgccagttcg
      481 gggaaggcgg ccatggcacg cagatagtgg cgcacgtagt cgccgtagcc gtcggtcagc
      541 cagttgtcgt ttctcgggta gcggttctta ccatcgtcat cgaccatgta ggtggcccaa
      601 ttcagttgtc tgatcgcgtc ttctttgcgc gccaagtcgc ctgtcttttc ggcgtacagc
      661 agctcgactg aggcgtatcg cgaggtgtgg ctgttgccgg gcaccttgta tgctgtctgc
      721 tcgttgatgg cgacgacgcc gtacttctcc cactcccggt tggcgaacat cttgaagctc
      781 cagccgagga tgcttttcgc ttgctcgcgc cagttggaat cccactccgg atgctcgagg
      841 atgtaccaag cgagggtgtc ggcattgatt tccgtgtccg accagcgtcc gacgtcctcg
      901 aagaagggcc cccacttgtt ggtcttcagc ggataggtct tgagccaagc gacgagagtc
      961 tgtcgcgtgc ggcggtaggt gtccacgtcg cccttgccta gcgcggcaag gtcctcaaac
     1021 agacgcagcg cccccgtcca gttggtcgtg tacgacgcga ggtagacgcc gccaccatcg
     1081 ctcttgacct gacgcacctc accggtgacc gcgtgaaccc ggtaaggcca gggtgaattg
     1141 tcattgtctc cgggtttcac gtgcctaacc agtgtgttcg cgatcttcac tgccgcgtcc
     1201 aggtagcgct ggttttgcgt catcttccaa aggaccacca gctcggcgcc gaagctcgcc
     1261 gccttgtccg gctggaggta gcccttgccc accatcatgt cgccgtcgta ctggcccgag
     1321 tgcaactcgg tgttgtaagg gtacgggaag ttggcccaca cgtcactggg cttcgacatc
     1381 ccgtggtcaa gccagtagtc ggccatgtaa atcatgttct gccggaccgc ggggtcgccc
     1441 aggtagccgt acaacaacgt ccacgacgaa agcgccatgt tgatctggtc gccaccgagc
     1501 ccacgtttgt cgtcctggtc cggcttccag acctggtgtt gcatatagta cggaacgccg
     1561 ttcgggcact ggcgcatctt gatccagaag ttccagacca actgtacgat gtggtcgtag
     1621 gccttcgccg gcgggccggc ccaagggatg atgcgaccgc tcgcatctgt ctggatgtcg
     1681 tgatagatca ggacctcagc agcgacagcg ggtgtggcgg ccagccccgc tagcgctaag
     1741 gcgagagcaa ggatgcggaa gttcatgttg ttctccagag aaggccagtc tcgttttctg
     1801 agagtttatt gtagccgccg cggaggttcg gttgctcggc ccgaagccgc ggctcgtcca
     1861 gagtgatgga cgctaaaggc gcatcggcac cgctgcgatt tgggacagcg gtagtaaact
     1921 ggttgggcta aaccgactgc ttccgtccac ggagggatta tgagcgctat cagtgactgc
     1981 caacgcgcag gcaaccgtct gacgcgccgc cagatctttt ccggcgtcgc ttactgctcc
     2041 gcctcagccg ccctatccgc tcggcctcgc gccgacgagc tctaccagaa gaagtcgtcc
     2101 caggtgattc ggatggggat cgtcgggggt aacttcggcg cgagtttctt ctggcacgag
     2161 caccccaact cgagggtgac ggccgtgtgc gacctgcgcg acgaccgcct ggagcgcctc
     2221 cagaaggtct accgctgcga gacgggttac aagaactacc acgagatggt cgcggacaag
     2281 aacgtggacg cgattggcgt gtttacgccc tggaacctcc atgtggacca ggcgattgag
     2341 gcgatgaagg cgggcaagca cataatcagc gcggtgcccg cgggcatctc gatcgaaagc
     2401 tgcgagcggc tgctctactg ggttcaaaag accggcatga cctacatgat ggccgagacc
     2461 agctactacc ggcccgagat catggcctgc cgccgctggg ccaaggaggg ccagttcggg
     2521 actatcttct acgctgaggg cgagtattac cacgacggca tcgaaaacct ctggtacgac
     2581 gagaggggcg agccgacctg gcgccgtgcg cggccgccgc tctggtacat cacgcacgcg
     2641 accggttgtg tgatcccggt aatgggcgag cggctgaccg aggtggtcgc ccacggctac
     2701 ggcgacatgg agcaccccgg cgtgctcggc gacggcctgc gcaccaactt ctactccaac
     2761 ccgttctgga atggcatagc gatgttcaag acgtcggctg gacatgcggt ccggattaac
     2821 gtcttccgcc acgtggccgc acgtgaggcc gagcgtgcac agttcttcgg caccgagatg
     2881 agctactaca tggccgtgcc ggggttgcac ccgaattccc gcaacgtgcg agagaagacc
     2941 ggcaagattc gcaccatcga caactacccg gtgaccgcga tccgcagcga gccattcgaa
     3001 ccggccaatt tccacgagat gctaccgccg gaactgcgca agccgagtgg ccacggcggt
     3061 tcgcacacct tcctgactca cgagttcgtc agcgcctgca tcgaacggcg gcggacggag
     3121 gttgatatct atgaggctct cgcctacacg gtgccgggac agtacgcgca tcgctcggcg
     3181 ttggaaggcg gcaccctgaa gaagatcaag aactacgaac ggccggcata aaagccgctc
     3241 gccacttcat cgcttggtcc cgaagcttga tccccgagcg cagatggcgc aggggatttc
     3301 cgcgctgtta gggacacccg ctcgacaata ggtcaagaag gaaaagggga tagcttgagg
     3361 aaggtgtcca gcccggtgaa caaaattcgg aaatcaaaat gcttgatctc gccccggatt
     3421 tcgcctagac ttgtgcgcaa gggggggacc accatgtaca aaaggagtat tgtatccgct
     3481 gctctcgcag tggtgttctc gtttgcattt cttgggagcc caagcttcgc ccagatcaac
     3541 actgggaggg tcacgggcac ggtcctggac gccaccggcg cggccgtacc ggctgtctcg
     3601 attcgagccg tgaacgaaga taccggcgtg actacctcga cggagtcctt ggctaccgga
     3661 gactacctgt tgaacttcct cgtgccaggc cgttaccggg tggaagccga aaagacagga
     3721 ttccagaaat cgttgcagac cgggatcgcc ataaatgccg gtggcatcac gcacatcgac
     3781 atccgcatgc aggtcgggga ggtgcgccag acggtcgagg tagccggcaa tcccatcgcc
     3841 gtctctaccg agacttcgga actgtcgcag acattcagca aaaccgacct cgacaagctg
     3901 ccgaatatcg accgcaatcc gctgtttcag atgaacctga tgccgggcgc caataatgac
     3961 cgaggaagcg ggaactacgg gaacaacggc ggagagaacg gctcggcgat cggcaatcag
     4021 cgcaatcagc tcgcttccat cgggggcgtg aacgccaacg ccaatagtgt gttcatcgag
     4081 gggactttca atcgggaacc gcagaacgcc tatgtcggcg tggtgccg
//
LOCUS       DATIFQ010000430         2762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2249629, whole genome shotgun sequence.
ACCESSION   DATIFQ010000430 DATIFQ010000000
VERSION     DATIFQ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2249629"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VLE22_21385"
     CDS             complement(<1..300)
                     /locus_tag="VLE22_21385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17019.1"
                     /translation="MMALVTFVETKLFTKLVQEYLSDAEYAALQQLLIVDPDAGDVIP
                     GSGGVRKLRWGVAGRGKRGGLRVIYFLRLRHGEIWMLTLYAKNVADNIPAKVLKKI"
     gene            complement(500..576)
                     /locus_tag="VLE22_21390"
     tRNA            complement(500..576)
                     /locus_tag="VLE22_21390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(540..542),aa:Arg,seq:acg)
     gene            complement(811..2493)
                     /locus_tag="VLE22_21395"
     CDS             complement(811..2493)
                     /locus_tag="VLE22_21395"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HSB17020.1"
                     /translation="MTISHWLIAVLITLGGASQAAVQPPQRAPGAAPGAAGCERLASS
                     AVPNTRIVLAQSVPAGAFDGPPAVFSGANLSSFYKSLPAFCRIVAKAQPSADSDIGIE
                     VWMPTSGWNGKLRGLGNGGFAGQMDYMQMGTAVKSGYAAAATDTGHTGGPVDASWAMG
                     HPEKVIDFGHRAVHEMTLVAKALVRAFYGHGPERSYFAGCSDGGREALMEAQRYPADY
                     DGILAGAPANDWTRLLTMSVMDSQALLVDPRSYIPPAKIAVIAAAVRDSCDEQDGLRD
                     GIVNDPRQCPFDPARLVCHSGEADTCLTDQQASALRKLYDGPRDSKGKLIFPGYSPGA
                     EEGDGGWITWITGPAPGKSVMLMFGLGYFSNMVYEQADWDFKTFTPEAGLEAAERKTA
                     AALNATDANLEPFMKHGGKLLLYHGWADPAIPPLNTIRYYDSVVSSLGQQRANSFVRL
                     FMIPGMQHCSGGPGPDWIGQDGVMEPGDPLHNANTALEQWVERGAAPAKLVATKLVMG
                     ESAGKARTTRLLCPHPTVARYSGSGDTGDAANFECIAAPAGQVGRGKKRSPR"
     gene            complement(2559..>2762)
                     /locus_tag="VLE22_21400"
     CDS             complement(2559..>2762)
                     /locus_tag="VLE22_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17021.1"
                     /translation="KPFVTVMFGNPYTALFLKELPAVMLTYDFYDLAEASAVRALAGE
                     APIVGKLPIGLPDMFPIGHGLTR"
BASE COUNT          526 a          913 c          816 g          507 t
ORIGIN      
        1 gatcttcttc aacaccttcg cgggaatgtt gtccgccacg ttcttggcgt acagcgtcag
       61 catccagatc tcgccatgtc gtagtcgcag aaaataaatc acgcgaagac caccgcgttt
      121 cccgcggcct gcgacacccc accgaagctt gcggacgccg cccgagccag gaatcacgtc
      181 acccgcgtca ggatccacga tgagcaactg ttgcagggcg gcatactcag cgtcagacag
      241 atactcctgc accaacttcg taaagagctt cgtctcgaca aacgtcacca gggccatcat
      301 acgtcactga cggactacgt caaagacgga actccatgat ggccacggtc aaactgttca
      361 ctcagcaggc gctgcggcgc ggggcgcatg ccggcaacat cttgaagttg aggaacttag
      421 gcggttttga gcacagaatt cgttccctgg tgggtcaggc cccgaactct ggctgcgaag
      481 ttgttgatgc ggtgtgagtt ggcgcgccct gcaagactcg aacttgcgac ctcctggttc
      541 gtagccagac gctctatcca actgagctaa gggcgcgtag ggctggtcta tcaggcagaa
      601 cttgtctgcc gcaaacggta attatatcaa agtcggttcc gagggcgctg aatgcaacca
      661 caggggctcc gaggcacaga ggtacacaga gtgaactcac ttaaagacaa atgctctgcg
      721 cgtctctgtg cccccgtgtc tctgtggttt cactcggcgc ctcgatttgt acctccatac
      781 ctgtggggtt ccactcggcg cccctcggat ctatcgaggc gacctcttct tgccgcgacc
      841 cacctgtccc gctggagcgg cgatgcactc gaagttcgcg gcgtcgccgg tatcaccgct
      901 tccactgtac cgggcgaccg tcgggtgggg acagagcagg cgcgtcgtac gcgccttgcc
      961 ggcggactcg cccatcacga gtttggtcgc cacgagtttc gctggagccg cgccccgctc
     1021 gacccactgc tccagcgcgg tgttggcgtt gtgcagggga tcgccgggct ccatgacgcc
     1081 gtcctgcccg atccagtccg gacccggacc gccggagcaa tgctgcatcc ctggaatcat
     1141 gaacagccga acgaatgaat tcgcccgctg ctgtccgagc gacgacacca cgctgtcgta
     1201 ataccggatc gtgttcagtg gcggaatcgc cggatccgcc cacccatggt agagaagcag
     1261 tttgccacca tgcttcatga acggctcgag attggcatcc gtcgcattca gcgcagccgc
     1321 ggtctttcgt tcggccgctt cgagccccgc ctccggcgtg aaggtcttga agtcccaatc
     1381 agcctgctcg tagaccatgt tcgagaagta gccaaggccg aacatcaaca tgacgctctt
     1441 gcccggcgcc ggccccgtga tccaggtaat ccagccgcca tccccctcct ccgctccagg
     1501 agaatacccg gggaagatca acttcccctt cgaatcgcgc ggtccgtcgt agagcttgcg
     1561 gagcgccgac gcctgctgat cggtgaggca cgtatctgct tctccgctgt ggcagacaag
     1621 ccgcgctggg tcgaaagggc actgcctcgg atcgttcacg atgccgtcgc gaaggccatc
     1681 ctgctcgtcg catgagtcgc gcacggctgc ggcaatgacc gcgatcttcg ccggcgggat
     1741 gtagctgcgg ggatcgacga ggagcgcctg cgaatccatc acgctcatcg tcagcaaccg
     1801 cgtccagtcg ttggccgggg cgccggcaag gatgccgtcg tagtccgccg ggtagcgctg
     1861 cgcttccatc agcgcttcgc gaccgccatc ggagcaaccg gcgaagtacg atcgctcggg
     1921 cccgtgaccg tagaacgcgc ggaccagcgc cttggcgacc agcgtcattt cgtgcacggc
     1981 gcggtgaccg aagtcgatca ccttttccgg atgtcccatc gcccacgaag cgtcgacggg
     2041 tcctccggtg tggcccgtgt cggttgcagc ggcggcgtat cccgacttca cggccgtgcc
     2101 catctgcatg tagtccatct gtccggcaaa accgccattg ccgagccccc gcaacttccc
     2161 gttccagccg ctcgtcggca tccagacctc gatgccaatg tccgaatcgg cgctcggttg
     2221 agctttagcg acgatgcggc agaaggccgg aagggatttg tagaacgagg aaagattcgc
     2281 tccggagaac accgccggcg gaccatcgaa tgctcccgcc ggcacagact gcgccagcac
     2341 gatcctggtg ttcggcacgg cggacgatgc aaggcgttcg cagccggccg cccccggcgc
     2401 cgcgccagga gcacgctgcg gaggttgaac ggcagcctgc gacgcgccgc caagggtgat
     2461 cagtacggca atcagccagt gagatatcgt catcacaacc tctctaccgg acgtctgagc
     2521 cccgaacgag gacctcccgt cagaatccgg aacccatttc agcgcgtcag tccatgtccg
     2581 atcggaaaca tgtcgggcaa cccgatcggc agctttccca cgatcggcgc ctcgccggcg
     2641 agcgcgcgca cggcagatgc ttccgcgaga tcgtagaaat cgtacgtgag catcaccgcc
     2701 ggcagctcct tcaggaagag cgccgtgtac ggattgccga acatcaccgt cacgaacggc
     2761 tt
//
LOCUS       DATIFQ010000431         5227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1484668, whole genome shotgun sequence.
ACCESSION   DATIFQ010000431 DATIFQ010000000
VERSION     DATIFQ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484668"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1139
                     /locus_tag="VLE22_21405"
     CDS             153..1139
                     /locus_tag="VLE22_21405"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB17022.1"
                     /translation="MKQAIITAFLGRLRDRFCEYQEPLTIAQKLETIAKIPGVRGVEV
                     VYPYEVDPPDVLKSQLDRLHLEVAAVNVNVKADPIFVNGSLSSPDPDVRRKALEFIKG
                     AKDYARAVGADKVTCCPLSDGYDYSFHTHYVRAWERMVEVVREAAAYIPEIPLYLEYK
                     PSETRVHCLLDSAAKTLLLCSAVDNPTLGVTIDVGHSIYGGETPAEALAHCAMSGHPY
                     YVHINDNNGKWDWDLMVGTCNLWAYVEFLFYLKELGYTGWVTSDTSPVRQDPLETFAF
                     NARLTARIWSWLDRIDRDVVRKHLERHEFLPIMKTFESELFARADDPARVTD"
     gene            1108..2424
                     /locus_tag="VLE22_21410"
     CDS             1108..2424
                     /locus_tag="VLE22_21410"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB17023.1"
                     /translation="MRTIPPASRISSPAARTHVRWQVIGFLFLFSFLTIVDRVAISAA
                     KGDMARELGISDVTFGFVFGVFALGYALFQVPSGWCADRFGPRLFLALIVLLWSLFTG
                     LTGLVHGAAVLIVIRFLFGGAEAGIYPTASRAIYSWMPRSGRGAAQGYLFVGSRLGAA
                     FGLAVVSWSVVHLGWRSAFYMLAAAGIVLAALWYLWFRDRPEDKPGLSEAELEYIRSD
                     REPASSGASEKLDWRKLLLSGNVLLLMYQYFASNFTFFLAFSWLLPYLREHYSLSVTE
                     AGGYASIPLYFGAVANWLSGLVVDGIYRRGHWRLSRWLPAVLGFALAVLSLVFAASMP
                     TVLGAVVCFSLATLGVDMTLSPSWTACQDIAGPHTGVLSGAMNMMGNIGSFVSSVTFP
                     ILVRTTGSAAAYFYLAALLNIVAILCWLRIRPDNLPVSPHPKRSAT"
     gene            2421..3233
                     /locus_tag="VLE22_21415"
     CDS             2421..3233
                     /locus_tag="VLE22_21415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB17024.1"
                     /translation="MRPNVKIGTTSYGFRYLLHDERRAPSLVSIVESARGYGLDCLQV
                     CENARPLDLSPRQWDDVLHSARNIGLEVRMGCMTLDLGTLDRYLERTAMIPDSSLRIV
                     LEETGRGRPARAELERFLDAAAPRLQAAGIALAVENHFDIPCRVLAEVAAPYPPSLIG
                     FCLDSANSLRNFESPNQVFDYLGSRAMFYHLKDYKVSGTNVGFTVTGATLGSGDLDLD
                     GFLEKVFVRTPEPLILLENWVPATGDWDTDVEEDSRWLRLAVESLSRMLERQ"
     gene            3308..4501
                     /locus_tag="VLE22_21420"
     CDS             3308..4501
                     /locus_tag="VLE22_21420"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB17025.1"
                     /translation="MRSHTTRRKFIGTAAALPAVGAARTKPEPTLAGAEVYTRFGVRP
                     FINGVGTVTVYGGSLMPPEVVRAMDEASRYFVMVPELQRKAGEHLAKLIGVPAAMVTA
                     GAASSITVATTACVIRNDPSKLPMLPDTTGLPNEVIQPKAHRSGYEAQIRLVGAKVIE
                     VETVEELESAIGPRTAMLFYMNRADGEGRISRQDFLRIGKERNVPTFNDAAADVPPVS
                     RLSSIVKEGFDLVAFSGGKGMRGPQSTGLLLGRKDLIEAAMPAISPAGGIGRGMKVGK
                     EEIVGLVAAVERFLRLDHQAEWRMLENCVADIIHVVSSVPGVKAVQEVPKIANEIPHV
                     AVSWDESERKLTAQQALEQLRDGEPSIAVLPRGKGQLLISVWMMQANEHKTVARRLRE
                     IFAAA"
     gene            4521..>5227
                     /locus_tag="VLE22_21425"
     CDS             4521..>5227
                     /locus_tag="VLE22_21425"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB17026.1"
                     /translation="MSDRISGLTRPTRREFLGLVPGALAAANAIAGVPAVREYRRGGM
                     VYRQLGRTGLDVSLLSFGSHTDPADKIKEGPGKTVLTEQGQGRRDRIIARAFDLGVNL
                     LDVYATEGQWEPAARMVKGRRNKILISLAHNTTPKDIDDACRLFGHVDLYRFHTAEID
                     GPTLGSWDIMRRAKEAGKIRAIGIATHIERTMTMALDELEGIDYLFFPYNFIHARADY
                     SQFLPAAIRQGVGLIAM"
BASE COUNT          943 a         1710 c         1525 g         1049 t
ORIGIN      
        1 accaacgggt acgtgggcta tctgcccgag caggaagcat acgccgaagg cgggtatgag
       61 ccggcagtgt cacactttga tcccatcgcc gaacacgtct atatcaaaga ggtggcggaa
      121 ttgctcgcgc agtttcgcta aacataattc ccatgaagca ggcgatcatc actgcgtttc
      181 tcggacggct ccgggaccgc ttctgcgaat accaggagcc gcttaccatc gcccaaaaac
      241 tcgaaacgat agcaaaaatc cccggcgtcc gcggcgtcga ggtcgtctat ccgtacgagg
      301 tcgatccgcc ggatgtcctc aaatcgcaac ttgatcggct ccacctcgaa gtcgccgccg
      361 tcaatgtgaa cgtcaaagcg gacccgatct tcgtgaatgg ttcactgtca tcgcccgatc
      421 cggatgttcg ccggaaagcg ttggagttca ttaaaggcgc caaggactat gcccgcgccg
      481 tgggcgccga caaggtcacc tgctgtcccc tgagcgacgg ctacgattac tccttccaca
      541 cgcattacgt ccgggcgtgg gagaggatgg tcgaagtcgt ccgcgaggcc gccgcttata
      601 tccctgagat tcccctttat cttgaataca agccctcgga gacgcgcgta cactgccttc
      661 tcgattccgc cgccaagacg ctgctgcttt gctccgccgt cgacaaccct actctcggcg
      721 tgaccatcga cgtaggccac tccatctatg ggggcgagac tccggccgaa gccctcgctc
      781 actgcgcgat gagtggacat ccttactacg ttcatatcaa cgacaacaac ggcaagtggg
      841 attgggatct catggtgggg acatgcaacc tctgggcgta cgtcgagttc ctcttctatc
      901 tcaaagagct cggctacacg ggatgggtca cttccgatac ctcgccggta cgccaggatc
      961 cccttgagac gttcgccttc aatgcgcggc tcacggcccg catctggtcg tggctggacc
     1021 ggatcgaccg agacgttgtt cgcaagcacc tcgagcggca cgaattcctg ccaatcatga
     1081 agaccttcga aagcgagttg ttcgcccgtg cggacgatcc cgcccgcgtc acggattagt
     1141 tcgcccgccg cgcgcactca cgtgcgctgg caggttatcg gattcctgtt tctcttttcg
     1201 tttctgacca tcgtcgaccg cgtcgccatc tcggcggcca agggcgacat ggcgcgggag
     1261 ttgggaatct ctgatgtcac tttcggcttt gtgttcgggg tctttgcgct cggctacgcg
     1321 ctgttccagg ttccttcagg ttggtgcgcg gaccgcttcg gcccaaggct atttctagcg
     1381 ctgatcgtgc ttctctggtc tctcttcacg ggactcaccg gtttagttca cggagccgcc
     1441 gtcctgatcg tcattcgttt tctgttcggc ggagcggaag ccggcatcta ccccaccgcc
     1501 agccgggcga tctacagttg gatgccacgg tcagggcgcg gggccgccca gggctatctt
     1561 ttcgtcggtt cgagacttgg cgcggcattc ggcctcgcgg ttgtctcatg gagcgtcgtt
     1621 cacctcggct ggcgctctgc cttctatatg ctggccgccg ctgggatcgt gctcgccgcg
     1681 ctctggtatc tgtggtttcg cgaccggccc gaggacaagc cgggtctgtc cgaagccgaa
     1741 ctggaataca tccgctccga tcgcgagcct gcgtcttctg gcgcctcgga aaagcttgac
     1801 tggcggaaac tgctcctctc cggcaacgtt ctgctgctga tgtatcagta tttcgcttcg
     1861 aacttcacat tcttcctcgc gttcagttgg cttttgccat accttcgtga acattacagc
     1921 ctcagcgtca ccgaagccgg tggctacgcc agcatcccgc tctatttcgg agccgtcgcc
     1981 aactggctca gtggccttgt ggtggacggc atctatcgtc ggggccattg gcgcctctcg
     2041 cgttggctgc cggcggtgct cgggttcgcc ctggctgtct tgagcctcgt cttcgcggcc
     2101 tcgatgccga ccgtactcgg tgcggtagtc tgcttttctc tcgccacgct cggtgtcgac
     2161 atgactctta gccccagttg gaccgcttgc caggacatcg ccggacctca cacaggcgtc
     2221 ctttcgggcg ccatgaatat gatgggaaac atcgggtcgt tcgtcagttc ggtgacgttt
     2281 cccatcctcg tgcgcaccac cggaagcgcc gctgcctact tctatctcgc cgcgctcctg
     2341 aacattgtgg ccatcctgtg ctggctgcgc atccgcccgg acaacctgcc agtatccccg
     2401 caccccaaaa ggtccgcgac atgagaccca acgtcaaaat cggcactacc agctacggct
     2461 tccgctacct cctgcacgat gagcgccgcg cgccctcctt ggtctcgatt gtagagagcg
     2521 cccgcgggta cggcttagac tgcctccagg tctgcgagaa cgcccgcccg cttgaccttt
     2581 ctcctcggca gtgggacgac gttctgcatt ccgcccgcaa catcggcctg gaagtccgga
     2641 tgggttgcat gacgctcgac ctcggaaccc tagaccgcta cctcgagcgc acggccatga
     2701 tcccagactc ttcgttgcgg atcgtcttgg aagagacagg tcgaggccgt ccggcacgtg
     2761 ccgaactcga gcgcttcctc gacgcggcgg cgccgcggtt gcaggcggcc ggtatcgccc
     2821 tcgccgtcga gaaccacttc gatatcccct gtcgggttct ggccgaagtt gccgcgccgt
     2881 atcctccctc cctgatcggt ttctgtctcg atagcgcaaa ttcgctgcgc aacttcgagt
     2941 cgcccaacca agtgttcgac tatctcggca gccgcgcgat gttctaccac ctgaaggact
     3001 acaaagtcag cgggacgaac gtcggcttca ccgtaaccgg agctaccctc ggttctggag
     3061 acctcgatct cgacggattt ctggagaagg ttttcgtccg tacgcccgag ccgctgatcc
     3121 tgctggagaa ttgggtaccg gcaacgggcg actgggacac ggacgtggag gaagattccc
     3181 gctggctccg cctggcagtc gaatccctca gccggatgct ggagcggcag taagtcgatc
     3241 cgcaccggtt tgccaccgcc actccgaacc gcatatgatc tgtgtcggta tcgaaaggag
     3301 tacggctgtg cgtagtcaca ccacacggag gaagttcatc gggaccgcgg cggcactccc
     3361 ggcggttgga gcagcccgaa cgaagcccga gccgacgctg gccggcgcgg aggtttacac
     3421 ccgctttggc gttcgcccct ttatcaatgg agtgggaacc gtcaccgtct atggcggctc
     3481 tctgatgccg cccgaggtcg ttcgcgcgat ggacgaagcg tcgcgctact tcgtcatggt
     3541 tccggagttg caacggaaag cgggcgagca cctggcgaag ctaatcggtg tccccgccgc
     3601 catggtcacc gccggcgcgg cttcgtcgat cacagtcgcc accactgctt gcgtgatccg
     3661 caacgacccg agcaaattac ccatgctccc ggacacgacc ggtctgccga acgaggtcat
     3721 tcagccaaag gcacaccgct ccggctacga ggcacagatc cgcctggtgg gcgcgaaagt
     3781 cattgaagtc gagacggttg aggaactcga gagcgccatc ggtcccagga ccgcgatgct
     3841 gttctacatg aaccgcgccg acggcgaggg ccgcatctcg aggcaggact tccttcgcat
     3901 cgggaaagag cgcaatgtgc cgacgttcaa cgacgcagcc gccgacgtgc ctccggtgag
     3961 ccgcctctcc agcatcgtca aggaaggctt tgacctcgtc gccttctccg gcggcaaggg
     4021 gatgcgcggt ccgcagagca ccggcctgct cctcgggcgg aaggatctca tcgaggcagc
     4081 gatgcccgcc atcagcccag ccggcggaat cggccgcggt atgaaagtgg gcaaggaaga
     4141 aatcgtcggc ctcgtcgctg ccgtggagag atttctccgg ctcgatcatc aggctgaatg
     4201 gcggatgctc gagaactgcg tcgcggacat catccacgta gtgtcttccg ttcccggtgt
     4261 caaggcagtg caggaagttc cgaagatcgc caacgaaatc ccgcacgtgg cggtctcgtg
     4321 ggacgaatcg gagcgcaagc tcaccgcgca gcaggcgctc gaacaactgc gagacggcga
     4381 gccgtccatc gcggttctcc cccgcggcaa aggccagcta ctcatctccg tttggatgat
     4441 gcaggccaac gaacacaaaa cggtcgcccg gcggctgcgg gaaatcttcg ccgccgcgtg
     4501 agtcattgcg gaggatctcc atgagcgatc gcatcagtgg attgacacgg cccacgcggc
     4561 gcgagttcct cggccttgtc cccggcgcgc ttgccgccgc caatgctatc gccggtgtgc
     4621 ccgccgtgcg cgagtatcgc cgtggcggaa tggtctaccg gcagcttggc cgcaccggac
     4681 tcgatgtctc cctgctctcc ttcggctcac acaccgatcc cgccgacaag atcaaggaag
     4741 gtccgggtaa aaccgtcctc acggaacaag gccagggccg ccgtgaccgg atcatcgccc
     4801 gcgcctttga cctcggcgtg aatcttttgg atgtatacgc gaccgagggc cagtgggagc
     4861 cggcggcgcg catggtgaaa ggccggcgta acaagatcct catctcgctt gcacacaaca
     4921 ccaccccgaa agacattgac gacgcatgcc ggctcttcgg acacgtcgat ctctaccgct
     4981 tccacacggc cgaaatcgac ggcccgactc tcggaagctg ggacatcatg cggcgggcga
     5041 aggaggccgg caagatccgc gctatcggca tcgccacaca cattgaacgc accatgacga
     5101 tggccctcga cgaactcgaa ggcatcgact acctcttttt cccatacaac ttcatccacg
     5161 cacgcgccga ctacagccag ttcctgcccg ccgcgatccg ccagggcgtc ggtctgatcg
     5221 cgatgaa
//
LOCUS       DATIFQ010000432        50886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2826988, whole genome shotgun sequence.
ACCESSION   DATIFQ010000432 DATIFQ010000000
VERSION     DATIFQ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 50886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50886
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2826988"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1326)
                     /gene="rseP"
                     /locus_tag="VLE22_21430"
     CDS             complement(7..1326)
                     /gene="rseP"
                     /locus_tag="VLE22_21430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HSB17027.1"
                     /translation="MAVVQNIWWLLVLIGVMILVHELGHYWAARFFDVRVETFSFGFG
                     PRIFGFRKGETDFRFSAILFGGYVKMAGEQPGDESVTDPRGFLAKPRWQRLAIAFAGP
                     LMNIVLAIALLTGLFMFKYPKPVESDLSATVGHVLPDSAAAKAGIRDGDRIVKIGDLE
                     NPTWQDILVAEASNARRPIKVVIERDGRRIDCTVTPVLLEKLGIGFAGWAEQAEIQVG
                     EVSPGMPAEKAGLRRGDLLLSANGEPIRSQFKLREVVGACGGKPVEIEFIRDGLRQRV
                     TVQPEKASIDGPERWMIGVGPEQRVVFSRLPFVEALAESVHHNTRGATLIYQVLRGIV
                     ERRLSAKTLDGPIRIAQLSGDAARQGAYSFIMLMAAVSLNLAILNLLPIPVLDGGVIL
                     MLLVEMVMRRDLSLPIKEAVFKLGFVFLLGIMVFVIYNDIVKVLPPG"
     gene            complement(1332..2549)
                     /gene="dxr"
                     /locus_tag="VLE22_21435"
     CDS             complement(1332..2549)
                     /gene="dxr"
                     /locus_tag="VLE22_21435"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HSB17028.1"
                     /translation="MKRLTILGSTGSIGTSTLDVVRRNSEEYGVFALVAGRNLDVLIP
                     QIREFRPAVVVVATEKVLHDLRNRLAESGHPRSEWPELGWGPEASVRAAIAPEVDFLM
                     SAIVGVAGLEATHEAVRRGKKVGLANKEVLVASGKLVMDAARDARSELIPVDSEHNGA
                     HQCLRAGKRSEVLRLILTASGGPFRDTPREQLASVTPVEALNHPTWKMGPRITLDSAT
                     LMNKGFEVIEACWLFGLSPSEIDVVIHPQSTVHAMVEYVDGSIIAQVSATDMRMPIQY
                     ALTYPGRGPAPVPRLDWTVARRWEFSPPDLEKFPLLKLAYQAQAAGGSATCTLNAADE
                     IAVEAFLHGKTTFLAIAEVVEETLSRVAVREPRSVAEVLEIDRESRLVARSIIEERER
                     GAAAGVRRPVATI"
     gene            complement(2797..3690)
                     /locus_tag="VLE22_21440"
     CDS             complement(2797..3690)
                     /locus_tag="VLE22_21440"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSB17029.1"
                     /translation="MAKLGFLGLGIMGYPMARNLLRAGHEVALWSHTSEKAKQLADAE
                     KGKYCGTPREVGEFAECVFLCVGNTSMSESVILGDNGIAAGARPGTVVVDAGTISPSR
                     SRTVSAELAERGLHMLDAPCTGSRPGAEGGTLTFMVGGDKEVFERVRPYFEPMGKRLY
                     YCGGPGMGLHAKLTQNLVLSNIIESFFEGIVLSTKAGVEPQLMLDILSNSAAKCGLIE
                     YKAPFILDRNFSPNFPLKWMHKDIGLMLESGQELNVPLPLTALTEQMFRAALASGLGD
                     EDFCSAIKTLEAWAKVEVKRG"
     gene            3785..4792
                     /locus_tag="VLE22_21445"
     CDS             3785..4792
                     /locus_tag="VLE22_21445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HSB17030.1"
                     /translation="MTSVRPSVLVTKRIYPEAIEFLRYHADVDYADSDDGLPPAELLS
                     RARGRQAVVCQLTDKFSAQVIEQLEGVRVLANVAVGFDNIEIPAATRKGIFVTNTPDV
                     LTETTADFAFALLMAAARRVVEAHAFVHSGEWRRWTIDLLVGQDIHGKTLGIFGMGRI
                     GQAVARRARGFSMRILYHDPQPVSAMVERELGLEFVPKQKLLAKSDFVSIHVPLLPET
                     MRLIGAAELRQMKPTAILVNTARGPVVDEAAVAEALRAGIIASAGLDVFEREPEVHPL
                     LLELKNVVLAPHIASASVETRRRMSMMAANNAVAILEGRRPPNLVNTELWPDQAAGPV
                     P"
     gene            4860..6107
                     /locus_tag="VLE22_21450"
     CDS             4860..6107
                     /locus_tag="VLE22_21450"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSB17031.1"
                     /translation="MAHLKHSTHPLRPGTEVLTRGFDPSLSVGSARPALFRCSTYVFS
                     SPEAAERSFAVSTGRAHAEAGEHVELIYARLNHPNAEILEDQMVPLEPGAKAAAVFNS
                     GLAAIMTAFLTFARPGDTIVYTVPLYGGTQHLIHELLEPFGVTCVAVPAGNGFELDEA
                     IRAARNLRIVFIETPANPTMMMTDIRQAATAASLRGDGVVVMVDNTFLGPTFQHPLSV
                     GADMVLYSATKYLSGFSDLIAGAALAGDPDLIRKMRLRRAIFGNILPPDECWLLDGRL
                     PTVTLRMNRQSKNAQRIAERLAVHPKVRKVHYPTLFDDSEQVRIRLAQCAYPGALFSM
                     QLRGGKPAAFDFLRNLKIARNAVSLGGVETLACHPMTTTHSGLTPEEHRLAGITDGLV
                     RISVGIEDWRDLLEDFEQALEGL"
     gene            complement(6114..7109)
                     /locus_tag="VLE22_21455"
     CDS             complement(6114..7109)
                     /locus_tag="VLE22_21455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSB17032.1"
                     /translation="MNRVQAALRRYAAVLICIWLVLGAGGVIYSRFLSIPVGIAIPLL
                     FALLVEISLYFAPAFREFRETLAARYSAPRIAAGLVVGAVVPYLIYSLPTGQFRVSAI
                     TGIVLLATVAAGWYLAMPHKPASDFAFLAFLAGIVLWKVFPDLYPSPAPRIRIDILGQ
                     LMWIRTGVGAMLILRRIEGTGFGFVPLRGDWGIGLRHYVYFLPVGIPLALGTGLVRFR
                     PVPLEWWKVTPLAAATFFGMLWVVALSEEFFFRGVLQHQLSRLLANETAAVLIASVLF
                     GLAHLPFRQFPNWRFALVATIAGCFYGRAFSRAGSIRAAMVTHALVNTTTRMLFV"
     gene            7177..7821
                     /locus_tag="VLE22_21460"
     CDS             7177..7821
                     /locus_tag="VLE22_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17033.1"
                     /translation="MAEREASNEQERQNYTYRQTVVLEELTPSGARSGEYREVRDIIF
                     SPEGERTERIDGQPRNLLRSLRMTAEDFRDIREVQPLLLTPDRLWIYETRYRGEETVD
                     ETDCWVLQIRPRQILDGQRLFDGLLWVNRLDYSIVRMEGQAVPQILHTSRENLFPRFT
                     TLRRAFEGKYWFPVHTHADDTLPFRSGAQRIRMTIRYENYQRFAAESTVRFEKK"
     gene            complement(7887..9512)
                     /gene="groL"
                     /locus_tag="VLE22_21465"
     CDS             complement(7887..9512)
                     /gene="groL"
                     /locus_tag="VLE22_21465"
                     /inference="COORDINATES: protein motif:HMM:NF009487.0"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSB17034.1"
                     /translation="MAAKQIVYSESSRQAILRGVNQLADAVKVTLGPKGRNVVLEKKF
                     GGPTITKDGVTVAKEIELKDPLENMGAQMVREVASKTSDVAGDGTTTATILAQSIFRE
                     GVKAVAAGANPMALKRGIEQAVETVVEEIKKYSKPVSGEMIAQVGTISANGDTTIGDR
                     IAESMKKVGKDGVITVEESKTMETTTDMVEGMQFDRGYLSPYFITDPDRMECVVEDPY
                     ILIHEKKISNMKDLLPLLEQIARSGKPLLVVAEEVEGEALATLVVNKLRGTLNACAVK
                     APGFGDRRKAMLEDIAILTGGKAIMEETGIKLEGVRLDDLGRAKRVTVDKDNTTIVEG
                     AGSEKAIEGRIKQLRAQIDETTSDYDREKLQERLAKLAGGVAVIKVGAATETEMKEKK
                     ARVEDALHATRAAVEEGIVPGGGVALLRAASALASLKLSGDEQIGVDIVKRSCEEPIR
                     QISGNAGFEGAIVVEKVRNNGKPTFGFNAATGEYEDLVASGVIDPTKVARTALQNASS
                     IAALMLTTEALVCEIPEKKDKPMPHGHGPEMDY"
     gene            complement(9531..9830)
                     /gene="groES"
                     /locus_tag="VLE22_21470"
     CDS             complement(9531..9830)
                     /gene="groES"
                     /locus_tag="VLE22_21470"
                     /inference="COORDINATES: protein motif:HMM:NF001527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSB17035.1"
                     /translation="MKIRPLYDRIMVKRIEEKETVMGGIIIPDTAKEKPQEGEVVAVG
                     QGKRLDDGKLVPLDVKVGDRILFGKYSGSEIKLDAEEYLIMREDEVLGVIDQAKG"
     gene            10063..12315
                     /gene="lptC"
                     /locus_tag="VLE22_21475"
     CDS             10063..12315
                     /gene="lptC"
                     /locus_tag="VLE22_21475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HSB17036.1"
                     /translation="MRRTRPLLLLAILAVLCAVGVAFYVQKRFQLASTAPTPKSLPPG
                     TSATAEYWSWSHTQEGRSVVEVRAANYRQIREPSRFDLEKVELRIFDKDGKRYDQVSS
                     AHAQFDIEAGVLYSDGQVDITMGVPAEPAPAGRLVFIRTSGVTFESKTGRAFTDRLAE
                     FTFDQGTGKCVGASYNPEFRELHMRSQAEVNWRGRTPGSIPMKIEAGELIYKERDAAV
                     LLSPWSRLTRGGTLLEAGSSIVWLGEEAIRRVEAKDAHGSDRSEDRRIEYAAAVLYMT
                     FAASSQVERIEGEGNAKLSSISATTRTEVSSDRLDLEFDATRSDSVLKRAWAKGRSSV
                     QSTPVSTGSSKPAVTRTLRSDVIELTMAPGGREIASVLTHSPGTVEFVPDQAAQKQRK
                     LDAGQIRIEYAGGNRIRNFHATDVATCTETPAIAGGKKAPPPMLTWSKELTAEFDQQS
                     GRLARLGQSGDFRYEEGERRGRADRAVLEAASNLITLEGSARLWDAAGSTSSDRILLD
                     QASGDMTAVGKVASTRAPESRTGSAGLISSGQPLQARADRMSTTGQNRRIRYEGDAVA
                     WQGGNRIRADVIEIDRTGRVLTANGSVVSQFLDEPSGAESDRKPARSPGFTVVRAKEL
                     RYEDETRIAHYRGDVKMTRTATEVKAAELRAQLAAGDSGSRLEKALADGRVEILHSAP
                     GRNRRATGEHAEYYPGEEKILLYGGSPLLVDSQRGSTKGNQLTWFANNDRLLVDGRED
                     QPAMSRILRK"
     gene            12321..13061
                     /gene="lptB"
                     /locus_tag="VLE22_21480"
     CDS             12321..13061
                     /gene="lptB"
                     /locus_tag="VLE22_21480"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HSB17037.1"
                     /translation="MRKLQTCEISKSYGGRKVVDDVSILVEQGEVVGLLGPNGAGKTT
                     SFYTIVGLISPDSGRVMLDDQEITQLAMYQRARRGISYLPQEPSVFRKLTVEENLMAI
                     LETLPVSVHERRERLASLVEQLGLEKVKQNKGYALSGGERRRVEIARSLVIDPTFLLL
                     DEPFSGIDPIQVLELQRIIFDLKRTGIGILVTDHNVRETLAVTDRAYIINNGKIFRAG
                     SPEALGSDPEVKRVYLGEAFNLGVATQS"
     gene            13112..14701
                     /gene="rpoN"
                     /locus_tag="VLE22_21485"
     CDS             13112..14701
                     /gene="rpoN"
                     /locus_tag="VLE22_21485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HSB17038.1"
                     /translation="MSLLSPRLQLKVQQKQILTPGLVQMVTVLQLNRLELKDLITQEI
                     SQNPVLEEAVDDGTEELAPEEVQAILETERDAADPAPADQGILEVVNTAAELTEGVPE
                     TEVEAIDSRVAPAGNEPTTETVEAVEAQASTDPFEEIDFGSFFDDYLDPGYKSPASEQ
                     VDKPSFETFLSAPVTLSDHLDSQLSLLVLSDSLREAAELIIGNLDENGYLTVSLEEIA
                     GSCEHTAEELDEALRIVQSLDPAGVGARDVRECLLLQLESQNGRGGVAWTMISEHLKL
                     VESKQFKELARLLGRPMEHVEIADHVIRNLDPRPGLRYSGPGARQIEPDVYISKEGEE
                     YLIQLNDEDLPQLRLNRQYRRMLDRDQAPNKDVRNYVKERYASAIQLMKNIEQRKQTI
                     LKVCQSIVRRQTDFLDQGIDSLKPMMIKDVAEEIGVHPSTVSRAVANKYAHTPQGVFE
                     LRYFFSEAVQGPSGSATPLLILKRRVKKMIGEEDPTHPLTDEQITARLQGEGIHVTRR
                     TVAKYREDMNIPSTHHRRVRS"
     gene            14725..15273
                     /gene="raiA"
                     /locus_tag="VLE22_21490"
     CDS             14725..15273
                     /gene="raiA"
                     /locus_tag="VLE22_21490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HSB17039.1"
                     /translation="MKVTYTGRTEKLTPTLQKKLDTKFAKLAKLLDQKGGEREAHVIL
                     TVERHQQQAEITVQFHDHPLVGIGASSDQFTAIVTAADKLEKQVLKLRTKWRDTKRGP
                     KESWTVPAAPEAEEAATPEPAAEKRIFRVNQHARRKPMTLDEAILEMEENRDYLVYRD
                     AETDRLSVLLRRRDGHYDLVEA"
     gene            15287..16183
                     /gene="rapZ"
                     /locus_tag="VLE22_21495"
     CDS             15287..16183
                     /gene="rapZ"
                     /locus_tag="VLE22_21495"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HSB17040.1"
                     /translation="MPTRSASRQVNREPELVIITGLSGSGKVTVLKCMEDLGYYSVDN
                     LPVDLIQKFGELVKDSPHIRRAALLVDIREGEALKRFPDLYARIRRERPCTLVFFEAD
                     EATLLRRYSETRRPHPLGTEESVVRSLRRERRQLAPIRALAEHIINTSRFNVHELREF
                     IAEKFGGNRDQSRIMVNVTSFGYRYGVPPDSDLVFDVRFLPNPNYIPQFNKLSGRHPR
                     VARYIRSFPQTTEFVQRITDLLVYLLPHYTREGKSYLTIAFGCTGGRHRSVMMADEIR
                     RRLSRVGFRVKSTHRDIEKSGT"
     gene            16313..16582
                     /gene="rpsT"
                     /locus_tag="VLE22_21500"
     CDS             16313..16582
                     /gene="rpsT"
                     /locus_tag="VLE22_21500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSB17041.1"
                     /translation="MANTFSALKRVRQTGRKTEINRARKSRLRHQIRTFRRLLETKDM
                     SAANAALPGTFSIIDRAAKWGIIKKNTAARYKSRLTTRLKATAAA"
     gene            complement(16589..17650)
                     /gene="holA"
                     /locus_tag="VLE22_21505"
     CDS             complement(16589..17650)
                     /gene="holA"
                     /locus_tag="VLE22_21505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HSB17042.1"
                     /translation="MSPDQFLAQIQRQEPAPVYLFLGAEPHRRDQCRRALVERVLSPE
                     ERQEGLSHYDLDETSLSDVVDDARSLSLFAPRRVICASNAEAALPRGKAGGEDEKGGT
                     ARAAETLAAYVRDPTPLSVIVLEAGRYDLEGEDKQKAERVRKFYDCVPAVVEFPKLTV
                     QEARLLARELARHAGVTIDRAEIDTLVETVGADGMRVAAEIEKLRLYTGRSRKVQAED
                     VAALVPDSSASTIFVLVDALGRRDRMRALELLHKLVRQGEYLPLALSFLATLFRLALA
                     AKEKGLRTPQQVQQQLSGPGRPIWRSRAEQILHTASIFTREQLEAVLKKIFETDSALR
                     DANPDDRIVLEEFILRLAE"
     gene            complement(17653..18186)
                     /gene="lptE"
                     /locus_tag="VLE22_21510"
     CDS             complement(17653..18186)
                     /gene="lptE"
                     /locus_tag="VLE22_21510"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HSB17043.1"
                     /translation="MRRKILRPWLVVAVLPALWLGGCGYHVAGKADLLPKNIKTIAVP
                     AFTNATIRYKLTERLPSAITREFLSRTRYRIVADPAEADAVLEGRVALYNYYPTILDP
                     ATSRASAVQMSVVLDISLRERATGAVLYARSGLEARERYEISVDQVAYFEESDPALDR
                     LSRDVARTVVSAVLEKF"
     gene            complement(18183..19205)
                     /locus_tag="VLE22_21515"
     CDS             complement(18183..19205)
                     /locus_tag="VLE22_21515"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HSB17044.1"
                     /translation="MKTGTVAIVGGESLIGRELRELINEQKIARSVKLIGVDADTAKL
                     TSEGGEAAVITPLDETNLRDAQVTFLAGSPDSSRKAMELTRKSTRQGAIIDLTYATEE
                     DPSARLRAPVAEPPGYAPPPGTLHVIAHPAAIALSLFLSRLAGTPKLLNVIAHIFEPA
                     SERGQQGLDELQKQTVSLLSFRTLPTDVFDTQLSFAMLSRYGSEAPEALGSIEQRIER
                     HLATLLAGLRGVPMPSIRLIQAPVFHGHSISVHAEFEDPPDTEAIATALASPEVDVRG
                     VDVEPPTNVGIAGQGGIAVGSIARDRNNPRGLWFWIVADNFRLMAENATAVAASLLRT
                     EQRGEQ"
     gene            complement(19210..20103)
                     /gene="pssA"
                     /locus_tag="VLE22_21520"
     CDS             complement(19210..20103)
                     /gene="pssA"
                     /locus_tag="VLE22_21520"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HSB17045.1"
                     /translation="MGRLSLPERLIDPNSPDRRPRRAAYALPTLFTAGNALLGFLAIL
                     KSFQGALLAASGQLGHSPHFEVAAKAIGVAVFLDGLDGRIARMTNTTSDFGREMDSLA
                     DVITFGVAPAVLAVSWGVQFVDGTTGAFVADHLRQMGYFIAFLFLLCGAVRLARFNVQ
                     KNPVPKNPGKPDRKYFVGLPIPAAAALVAAIVFAADSAPIEYWPLALAWLVLMALLAF
                     LMVSTWRYRSMKDVQLLAPRSPLTVIVWGGFFYLIWNFPEPVLLAMAVTYVGSGIVVR
                     LGGIVKRRLRHPVPRTESLPG"
     gene            complement(20091..20762)
                     /locus_tag="VLE22_21525"
     CDS             complement(20091..20762)
                     /locus_tag="VLE22_21525"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HSB17046.1"
                     /translation="MVLDGIYYALALTAGGGVVSYFTRPAWGIPLYLLAAFCLYFFRD
                     PERPIPAGAVAVSPADGKIVAVKKEGPSHTRISIFLNIFDVHVNRAPISGCITDVQYK
                     PGRFLVASREEASSQNEQNEVTIQGDGTRVVFKQIAGLIARRVVFKKKTGDVVQTGER
                     VGLIKFGSRVDVVLGPEWAIQVREGERVMGGSSVLAWRCEFAAPALRAGERMTPEGLE
                     PGWAD"
     gene            complement(20823..21962)
                     /locus_tag="VLE22_21530"
     CDS             complement(20823..21962)
                     /locus_tag="VLE22_21530"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HSB17047.1"
                     /translation="MDSHLSFAQSRQSEIVGFIRRLVECESPSDDPAAVNCFVELFAG
                     AVSDIAETRTFPGGRFGNHLRCEFELPGRRKSGQILAIGHSDTVYTTGTLARMPFREA
                     DGRIWGPGALDMKGGLAFFVFAIRALRALETPVSHRVVLLVNSDEEVGSDSSRALIER
                     EARKSEAVLVLEPATGLEGKLKTARKGVGDYTMIVRGRSAHAGLDFSSGANAIVELAQ
                     QIGKVSRFTKLERGVTVSPGVISGGTRSNVVPDEARVTFDIRAPRASDAAALDRKFRA
                     LKPVDKRCIIEVAGGVNRPPMERSASVVRLFRTARRLAGELGRELEESTSGGGSDGNF
                     TAALGVPTLDGLGAVGEGAHSPRENILADRIADRTALLAKLVAAL"
     gene            21987..22439
                     /gene="fabZ"
                     /locus_tag="VLE22_21535"
     CDS             21987..22439
                     /gene="fabZ"
                     /locus_tag="VLE22_21535"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HSB17048.1"
                     /translation="MPVLDIEAIRNILPHRYPMLLVDRILELEPERIVGIKNVTVNEP
                     FFAGHFPAFPVMPGVLIVEAMAQVAGVLVLKEIGDRQDKLVLLASIEKAKFRRPVLPG
                     DQLRIEMKVGKRKASVALMYGEATVDGVVVAEAEVLCKLADREQKKNP"
     gene            22450..23226
                     /gene="lpxA"
                     /locus_tag="VLE22_21540"
     CDS             22450..23226
                     /gene="lpxA"
                     /locus_tag="VLE22_21540"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HSB17049.1"
                     /translation="MPVHPTAIIHPQARVAESADIGPYCVIGAEVSIGAGTRLMAHVY
                     MEGPTAIGEDNTFFPYSTVGVASQDLKYKGERAETRVGNRNRIREFVTIHRGTEGGGL
                     LTEIGDDNLLMAYVHIAHDVRVGNQTVLANGVTAGGHVLIGDWAVVGAFTGLHQFCRV
                     GRHSMIGGYSVITQDVMPFATIVSDRETKVFGANKTGLERRGFPDASIDAINKAIRLL
                     TRSGLNVSQAIDRIQSEMPACSEVEEMLEFIRGSERGVIR"
     gene            23244..24056
                     /gene="lpxI"
                     /locus_tag="VLE22_21545"
     CDS             23244..24056
                     /gene="lpxI"
                     /locus_tag="VLE22_21545"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HSB17050.1"
                     /translation="MKYALIAGNGRFPILALEEAGKLGHEVVAIGIREEAAPEIETLA
                     SRCFWISLGQLSRLIEILKSEGVSEVVMAGQVKHAQIFSSIRPDWRLLKLLTSLRQKN
                     TDALIGGVAKVLEDEGIRLVDSTFLLKPLLAGEGVLSRRKPEDDEEADLAYGRRVGNA
                     LADFDVGQSVAVAERACVAVEAMEGTDAMLRRAAGLVNGRPLRLVKVSRRRKHLLFDV
                     PVAGPATIAVMRETGTTALSVDAGRTLLLDRDEMIGAADAAGITLIGCPPEE"
     gene            24134..25096
                     /locus_tag="VLE22_21550"
     CDS             24134..25096
                     /locus_tag="VLE22_21550"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17051.1"
                     /translation="MNKIRAAVVGAGQFGRNHMRVIHGSPRAELVAVVDIDPERAAEA
                     AAPYGCRALTDLAALNGSVDAAIVATPTSAHVDVALALMNAGIDVLVEKPIARDLTSA
                     RYLAEAAESSRRILQVGHLERFNPAVSALSANVRLPLFFEVHRLSQFTPRSLDIDVVL
                     DLMIHDIDIVLSMVGCMPEEIRAAGISILTPKVDIANVRLSFPGGCVANLTASRVSTE
                     RVRKLRLFQPSEYLSIDYGRQEAFSLSVTPDRRIAPRTLTVQKNEPLQLELEHFFESV
                     ETRRPPRVGGRDAVRALEVALGILGKIEEHSRVVAQSLASAQKG"
     gene            25106..26167
                     /locus_tag="VLE22_21555"
     CDS             25106..26167
                     /locus_tag="VLE22_21555"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSB17052.1"
                     /translation="MDPPLQAYDEELRLFPDWKSQEVSVRSLRPFAASLVLHAIAISG
                     LLLLPAGSVLDRSGPRTLAQLPEPFKLVEPPLELTQTEPNRGKASKSVDLEALLPRPP
                     LQLPPALPSTTRPAARVLAPPSPPTPEVAALPEPPKIDTAGSRLAQPSVQPPGSLTAS
                     PPQIQTEEKPKLAFERPGSPAGEPGSEGRQLARLAPPGGSVSEVGRELAGGRSAGGLV
                     VGDLGEGIGGLGEGLNLPPSPGRSGSSLELLSDPMGVDFRPYLIKVLSAVRRNWFAVM
                     PESAKLGRRGRVVIQFAVSRDGSVPKLVIATPSGTDALDRAAVAGVSASNPFPPLPVE
                     FRGGSVRLQFVFLYNLKSN"
     gene            26259..26411
                     /locus_tag="VLE22_21560"
     CDS             26259..26411
                     /locus_tag="VLE22_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17053.1"
                     /translation="MKGRLLAATLCYVILGAVATLTLGGKPLAFVLLFLAALAVKTWI
                     HSVRSE"
     gene            26692..27450
                     /locus_tag="VLE22_21565"
     CDS             26692..27450
                     /locus_tag="VLE22_21565"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HSB17054.1"
                     /translation="MNATRVSGRGSENPNSKRDRIILDHLPLVKAIALRVHENLPVHV
                     DLDDLVHAGILGLFDAAKRYNPSKRVNFPSYAKHRIKGAILDSLRQLDWASRDMRRRH
                     KQIEAATRDLAAVLKRNPTQAELAAKMGVGMDRWQQMMVEMRNVGLVSASGRGPENED
                     GPPPDFPAEEETQPDSICAREQLRGHLRVAMGTLPERYRKVVLLYYTNEMTMREIGEI
                     MGINESRVSQIHKSALQKMAEALQSAGITSSGAF"
     gene            complement(27463..29178)
                     /gene="sppA"
                     /locus_tag="VLE22_21570"
     CDS             complement(27463..29178)
                     /gene="sppA"
                     /locus_tag="VLE22_21570"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSB17055.1"
                     /translation="MVKFLIGVLTGVILAGLALIILLFAAVRLAERPPAIADGSTLVL
                     NLEGDIPEQAPMSIPLPFFEEKEPLTVSDVWRILRRAETDPRIKAVVLLPGGLNVGWG
                     KLQEIRGCLARFRKSGKPLVAYLRSARTREYYLASAADRVYMAPEDWLDVKGLRAEVM
                     YLRKTLDKIGVQVEIEHAGRYKDFGDMFTRTSMSPETREVLNSILDELFAHLISTVAE
                     GRRKDPNEIRAAIDDGPFLPEEALKKGLVDALVYEDQVYSELRKRLAQDDLKKLGCRD
                     YLRAVAAERPAGRRIALVVAEGSIEGGSGDDLSGESGIYAQRFIRLLRDVAKDEAIRA
                     VVVRIDSPGGSSFASDEIWREMNTLSRAKPVVVSMSDAAASGGYYMAMTGDPVVAYPG
                     TITGSIGVVFGKVNLRGLYDKLGIQKEMLTRGRFADLDSDYQPLSDAARKKLRDGIDD
                     NYRTFVQKVADARKRKFDEIEPLAQGRVWLGSQAKVRGLVDELGGIDQAIELARRKAQ
                     VPDTQRVNLVVYPPKRSIVERLLSKSKVSALESRFRSLTKGVHINAWIEGGMLRLMPY
                     SVEIK"
     gene            complement(29195..30049)
                     /locus_tag="VLE22_21575"
     CDS             complement(29195..30049)
                     /locus_tag="VLE22_21575"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB17056.1"
                     /translation="MTRRTFLGSTAAAAMASAAPARLPIKKAIWFGMLPEKLSIMDRF
                     KMARDAGFEEMEPNTETDPRKAEEFKKASDATKVRIHSVMNSAHWKYPLSSADPAVVA
                     ESMKGMETSLKNAQLWGADTVLLVPAVVNPQTSYKDAWTRSQVQIRKLIPMAEKMKVV
                     IGIEEVWNKFLLSPLEMARYVDEFKSPWVRAYVDVGNMVFYGYPQDWIRTLNKRICKL
                     HFKDFRFKNMKADFVNLREGDIDWKEVYKALAEVGYKGSATVELEGGDLPYLKDVSRR
                     VDLILTGA"
     gene            complement(30106..31026)
                     /locus_tag="VLE22_21580"
     CDS             complement(30106..31026)
                     /locus_tag="VLE22_21580"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB17057.1"
                     /translation="MSYRPTRRSALAVMAGAAVPFHASGRPIRLGGPIFLKTDDPQEM
                     AREHRRLGYSAAYCPNAKPDDTARVREIEKAFAAERVTIAEVGAWVNMLDPDPQKRQQ
                     NMRFVTGRLALAEAVGARCCVDIAGSMNPKVWYGPHPGNFSKEFFDATVENCRRLIDE
                     VKPTRTKFTLEMMGWSLPDGPDAYLRLIKAIDRKAFAVHMDVCNGINSPERFYQSGKF
                     VEECFRKLGPWIVSCHAKDLAWVVEMNVHFVEVVPGRGEVDYRSYLRELAKLPSEAPL
                     MLEHLKTAEEYEEGKRYIRQVGADVGVEFA"
     gene            complement(31107..31772)
                     /locus_tag="VLE22_21585"
     CDS             complement(31107..31772)
                     /locus_tag="VLE22_21585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB17058.1"
                     /translation="MAKIRILLADDHTLFRQGVKTLLAAESDIEIVGEAANGTEALER
                     AGQFRPDVVLMDIGMPGPSSFETTRQIRRNRPETKVLFLTMYDDEDYLVEGMEVGASG
                     YVLKDSPAPQLVAAIRDVSRGGSYLSPRMLSQLVDDFRTRIKSTDRLPRFATLTPREK
                     EVLKMLAEGNSVKEVACQLNLSVKTVEAHKFNLMRKLDIHNKAQLVQYAIQKKIIKIP
                     NLI"
     gene            complement(31777..33426)
                     /locus_tag="VLE22_21590"
     CDS             complement(31777..33426)
                     /locus_tag="VLE22_21590"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HSB17059.1"
                     /translation="MTYSENPPALAAAADVLGEQLRAHLERLAQILKGRAASLERKFL
                     MHLRRAGYEAVQRRALAAITPGAASRLLAAGKPVVDFFEQVEYNGRRLAKLNLAPDQI
                     ITALGEYDRLLAPLFRPLPAQERANLGWAREQLQFCVVLTLNNAFYQVREAETSAYQE
                     LFRAELESKTMDELLPRMIETLTRFCRARAGALFLRDWDSGDWILRAAVEGTDKLEAR
                     PLRVTGTQFRSGKLSVPACRCGRVEGLALDPKWRGRYRSCWSVPLAAGDCAVGVIQFG
                     FAGPYEWLPREQDLLAAAGERCLLAAEKARLVEDLAAREEQVRALGATVVATEERERR
                     RISAELHDEAGQSLLCIRLQLELLERLIPESLEYLKTGLAETRALTERTISEIRRLIG
                     ALSPAVLEQLGLPAALRQIATRLRRFHGMEVRVRTSNLTELPERVAAVVYRLIQECAN
                     NIARHSSAGCVNISAVHADGRLRLRVADDGVGFRVEEKSGKSESHGLPGMRERVALFR
                     GEFQIQSRPRKGTQVLIELPVTPEIDRNGPKIPDCPGRNRA"
     gene            complement(33453..34679)
                     /locus_tag="VLE22_21595"
     CDS             complement(33453..34679)
                     /locus_tag="VLE22_21595"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSB17060.1"
                     /translation="MRLAVLVVAAGIVPFVEAGELHDAARAGDAAKVRALLDGGADIN
                     ARDSLGGTALHDAAWGGHLDVIRLLVERGANVNARHAEAGSTPLHYAVVTNHREAAAL
                     LLDNGADIRARYSSGQTALHLAANRGYRDLVELLLNRGADVNEKDNSGSTPLDEAAWK
                     GQAEIARLLVARGADVHVKNRESGTTPMHEAAVKGHAEVVAILLEAGADPEAKDGSGA
                     TPLDEALRYRNNRVAEVLLDRAGKQPLGRGAVERLQQAVLGGQTDLVRMLVERGISVT
                     GNTTNGSTLLHDAALNGHAEIAALLIAHGAGVNARNSAGGTPLHDAALGGHREVVALL
                     LGRGADVNAQDSDSGATPLHHAASWGRLEVVRLLVENGADVNARTRGGKTPTTAAMEN
                     GYAGIVAFLKEKGAKE"
     gene            complement(34710..36521)
                     /locus_tag="VLE22_21600"
     CDS             complement(34710..36521)
                     /locus_tag="VLE22_21600"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB17061.1"
                     /translation="MNTHVNSNNFPSRRQILGGAAGLALAATGCRRGPKQTRPNILWL
                     IAEDLSTDLGCYGNGLAHTPNIDRFATEGVRFTNAFVTAPVCSASRSALATGMYQTSI
                     GAHHHRSHRSDGYRLMDGIDLFPHYFRKAGYHTSNVLTAAAGVKGTGKTDFNFQVDKV
                     FDGTDWNQRGEGQPFYAQINFSETHRAFHRFTERPTDPAKVELPPYYPDDPAVRLDWA
                     LYLDSAQHLDVKVGKVLERLKEEGIEESTIVIFFGDNGRPMPRDKQFLYDGGIHVPLI
                     VRVPERFRPEAYQPGSVRDDMVSAIDIPATSLRLAGIEPPIHMQGQVFLGSGEKSRDF
                     IVAARDRCDETEDRIRCVRTKKYKYIRNFFPERPYAQPNVYKDTSYPTLQAMRQLDKD
                     GKLTGPPALFLAARRPPQELYDLEADPHEINNLAALPEQEKTLTDMKNRLDRWIRETG
                     DQGASPEKALPEEYKYRAQADGWCGKGQCVLSKTAGALRVQCSGKGNEVARSWVAADG
                     EMAVRFLARSKDVPVRAFAWSTIEDMASNPKNRASVDFAADGQWHEHLVSFKPGGYLA
                     LLRFDLGDAGGTIEFDWIRLYANSKGEGVPAVRWEFA"
     gene            complement(36527..38311)
                     /locus_tag="VLE22_21605"
     CDS             complement(36527..38311)
                     /locus_tag="VLE22_21605"
                     /inference="COORDINATES: protein motif:HMM:NF027656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HSB17062.1"
                     /translation="MAIFLASAAILPAQSLEPLETLRKGHPRIIALDSDIDRIRTLIA
                     ADEGARRVYASLVERARKLEGEPPVEYKVVGPRLLAQSRRCLDRIYTLALLYRLDGER
                     RYLDRAVKELRAAAYFPDWNPSHFLDTAEMTHAFAIAYDWLYHGLTPEERAWIRRAIV
                     DKGLDQAIPIYAKQGWWVTSKYNWNQVCNGGIGIGALAIAEDEPQRASKVLRNAVKSL
                     PAAMASYAPDGGWAEGPGYWHYATRYAVYFLAALKTALGIDFGLSASQGFDRAGHFRI
                     YSTGPSHRSFNYADASDRVGPAAEMFWLAQRFAQPAYAWQQHQLLKTSGGDALDLVWY
                     RTEAESPKRAGWPPHALFQGVQVAFLRSNWESTDGIFVGVKGGDNKANHGHLDLGAFV
                     LDAGGVRWAIDLGPDDYNLPGFFGKQRWNYYRLRTESHNTLLIDDENQDTRAEARIIG
                     HRFRPNSAWVRIDLSQAYPGKVKRMDRTLALVQRKHVLVLDSLEATEPVQALWGMVTD
                     AEVSLDGAKAELRKGGWTLAAEIRSPAEATFDVVSTAQPPPQAKNEGTRKLVVRLPEK
                     VTKLALEVWLTPHRSGKPKPVVPANPKL"
     gene            38402..39337
                     /locus_tag="VLE22_21610"
     CDS             38402..39337
                     /locus_tag="VLE22_21610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB17063.1"
                     /translation="MERRDFLKSAVAGTIAAREAAAKKGEMPKRKYRDNVKLSVIGFG
                     GIVVCGMEQNLADRTVAESWDRGINYYDVAPTYFDGEAETKLGIALKPYRKKAFLACK
                     TTIRDAQGAAKELEQSLKRLHTDHFDLYQFHAVSSMEDVEKILAPAGAAELFTRARKD
                     GKVRYLGASAHNAEAAIALMERFPLDSILFPVNFVCFQQGSFGPQILAKAKEKGVARL
                     ALKALAHHPWPKGTDRKKSGYEKCWYKPVDDPELARKALRFTLSEDITAAVPPGDERI
                     YAMALDIAAAFTPMTAAERQELLASSQGTAPIFKV"
     gene            39503..41506
                     /locus_tag="VLE22_21615"
     CDS             39503..41506
                     /locus_tag="VLE22_21615"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB17064.1"
                     /translation="MRVLASFACVVALLAMGACRRSGKGGGTTAARRINVVLVTIDTL
                     RADRLGCYGYAQVATPNLDKLAQSGTLFENAVTHTPLTAPSHASIFTGTYPTVHKVRD
                     TGGFVLQGPHATLAEILQRNGWATAAFVGASVLKKGFGFSRGFTVYDDQMPKLEPGRP
                     AGDYAERRAGEVVDRAVEWLQRQDDKPYFLWVHVFDPHSPYDPPSPFREQYRGRAYDG
                     EVAYSDRELGRLFDAIARKSGGENTLVVVLSDHGESLSEHGEYSHGVFLYDATLRIAF
                     VMSGPGIPKGMRVKQQARAIDVLPTILELIGGNLPAGVQGGSLVPAMAGKTDPAAWSY
                     AESLYPKINMGWAELRGIRTNRWKYIRAPKPELYDLSRDIAETANVVSQHPEEVRDLE
                     ARLRDVAAEGEKVETTMVDRRTMEQLKSLGYVGGSSRTQYTLKGEGADPKDRTGILKL
                     LYLAISPDWGGKMSQRIPTLRRALAEDPGNPTIYYHLGDEYDKAGRSAEAMKLYQEGI
                     RNGVRNAWLYSRLGNLYIRQGNHAEAITAFERAAKLNPSDCESLSDLGMAYLDTGRPT
                     DAERVYKWCLATEEEYAPAYNGLGLVAVQRQDMTAARGYFEKAVEYDPDLLEGQLNLG
                     RIYKMIGANARARACFEVFLAKATPAEYGHIIPKIKAELAQLQ"
     gene            41718..45422
                     /locus_tag="VLE22_21620"
     CDS             41718..45422
                     /locus_tag="VLE22_21620"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB17065.1"
                     /translation="MRVSSTKLNILFCLFLAVLLAAPALPQASTSAVNGTVRDQSAAV
                     IPAAAVTLTNTATNVTSRTTANQVGFFLFTGINPGPYRITVEAPGFQKFEGTLTVQVQ
                     QAAVVNITLNVGQTATEVSVQDVTPMVTTDSPTLGHTLERTRIEQLPINGRALTSLLQ
                     TVPGMEGTRAFGLRQMSFEMVLDGAALADRYSWNTVTPRQPGLDSVQEFRVENNASSA
                     KFARPTSIIVTTKGGTNEIHGTAFETNRNSGYGVARQRQDTWEKASFLNRNEFGFSVG
                     GPVYLPKIYNGKNKTFWFTSTEWYREISSMTLQASLPTQAMRDGNLAGLTNDAGNAIT
                     IYDPWTTDSTTWARQPFPNNQLPQSKQSPLYKYLIEHTQLPTRPEVNGYSASNYVGSW
                     SPFNYQWTTSSRIDHRFSDKDSFYGRYTQGNYNRIGQFYGIPSKNWDVVPGNTQTNLS
                     PNKSFAFSEVHTFSPTFFNELLVTGTRTRFDVATGDPTRMYDSELGLPNPFNVKQWPG
                     LYNLGFDGSYLFETQNGNGFYAFYGILDDNATKIVGRHELQFGFHFRSDQMNLLPQQQ
                     QGAGSFSWASTGTALYDPSTSRTNPGTTPFTGDQFANFYLGIGRYNNQLNRGMFYARS
                     KEYAGSFQDNWRVTPRLTLNLGLRYDLFPPYYEKNNAVSSFDPSDKSVVLGAPLEKLY
                     QLGYTFPAIVDRLSQMGMKFKTYQEAGLPEHMINTSKDGWGPRIGFAYRMGDGAKSLV
                     LRGGYRISYFHFVMGGWAARMRMNAPMTARFYGPNGDLEQSAYSPDGIGNYLLRSVPV
                     YISGQNTTNDLVQPTNARGLARGCCGVSYFNTDMPDPRVQDWNFTIEKEIMENTVVRA
                     GYFGNHSSRLEQLYQFNNATPDYVWYMTTGKRLPTGEYSQTARRFFDQTTYGTLERWQ
                     NTGWGNGNGIQLEAERRYAKGYAFQLFYVLTNSMMAGGGGYSGTSPIPEVNQFMPGAV
                     PTDIDARNAFLNYQRDTSVPKHRVRWNFLVDLPFGKGKPFLGDAGSWLNRLVGGWQVA
                     GIGSLQSTYLTLPTGLVTNGNKIEVYGYKYPIEDCTSGKCYPGYLWWNGYIPAYRINS
                     TDPKTGKPNGYMGVPADYQPAVQPLYPYPADYLTRSGTTDPMYPWYGGNTTWITLNDG
                     TRQRTTWGELPMLRNQYLPSVLQWGLDASIFKAVPITERFNVRINADFFNVLNHPGNP
                     NSFLGSTGILNTRNSGSGARQIQLTLRLAW"
     gene            complement(45487..45563)
                     /locus_tag="VLE22_21625"
     tRNA            complement(45487..45563)
                     /locus_tag="VLE22_21625"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45527..45529),aa:Val,seq:tac)
     gene            45622..46575
                     /locus_tag="VLE22_21630"
     CDS             45622..46575
                     /locus_tag="VLE22_21630"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HSB17066.1"
                     /translation="MTSAILPEHIRAAAERIRPIAHRTPVLTSRSFDGEASIRAFLKC
                     ENFQRGGAFKIRGASNFLFSLAPEQLRRGVVAYSSGNHAQAVAIAARATGASATLVMP
                     ADAPRSKMEATRAQGATIVTYDRHRESREEIGSRIAAETGATLVPPFDHPWIIAGQGT
                     TALELLEDAPELDALITPVGGGGLLSGSAIAAKSVRPAIRIFGAEPERANDTWQSLSA
                     GRRVEIPTPDTIADGLRATRPGEITFPILQRHVEEILLVSEDEIKATVRFLLSRLKIL
                     VEPSGAVAAAAVLYRKLPSNIESVGIILSGGNVDFETLQAL"
     gene            complement(46563..47642)
                     /locus_tag="VLE22_21635"
     CDS             complement(46563..47642)
                     /locus_tag="VLE22_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17067.1"
                     /translation="MAETEYRNRRAFQIETASLRVTVTAEGGHIAEVLHKATEVNPLW
                     TPPWPSIEPSTYNRVKHPEYGDDAESKLLAGIIGHNLCMDIFGGPSPEEAAAGLTVHG
                     EASVVPYEISADSSEMNCRAELPLAQLRIERKIRLSPGSGVVRIVETVENLSVFDRPI
                     AWTEHATLGPPFLEKGLTEFRASATRSKVDEEDSTGGKGYMKTGAEFNWPHVPRNNGG
                     TSDLRVYTSAAVSGGFTTHLMDPARTQAYFAAWSPSTKVLFGYVWSRADFPWLGIWEE
                     NYSREQPPWNGKTLTRGMEFGVSPIPEPRRLMIERNTLFGERCYRWIPSLSKARVEYC
                     AFIGTADAIPENVSWDGGWNVSYNA"
     gene            complement(47700..49103)
                     /locus_tag="VLE22_21640"
     CDS             complement(47700..49103)
                     /locus_tag="VLE22_21640"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17068.1"
                     /translation="MPAKKDPTRRTFVKTAGAVTAAAAASSFQGPLIQKVKAAGDQVL
                     YGVIGTGGRGTYLLKHMAGLDNGRCVSVCDVFEPNLKNGVQTAGTNPTAYKDYRELLA
                     RKDIDAVVIAVPLYVHFPVTRDALLAGKHVFCEKSLVFKPEEVHALRTLVNERPKQIL
                     QVGLQRRYSKFYQTAKQMVDKGMLGNVTHVMAQWHRNPGWQMKPDPVRQRERNWRLFR
                     EYSGGLTAELASHQIDVADWMFGAHPEFVVGVGGLEFIKDGRDVYDNIQLIFRYPRGQ
                     KLIYTSISTNKQLPLFGSTRTEFGERIMGTGGTIEITVGTDDEPSIGMWYIEPKPPQV
                     TPAKTAKKEEVVAGATLASTGRGAKGFPILLERDQFSGKESFLEKELKFARRWLYSKG
                     VMVPEESRNPVDVQMESFFESCKTGARPKADLNIGLQDSTAVMLANLAMDEGRRVYFN
                     EMEQMGRTPGAVPANKS"
     gene            complement(49195..49989)
                     /locus_tag="VLE22_21645"
     CDS             complement(49195..49989)
                     /locus_tag="VLE22_21645"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSB17069.1"
                     /translation="METEVHVYAFSVAASVLLSFFPFLIVMLSLCKHVLKWPAAVTAV
                     NLALSDYFPGNLGEFVHRNLHATVEQRGPFQIVSVILLLFVANGIFEPLEVALNRAWG
                     VAKHRSFLRNQVISTGLIFACGTLALASTTLTALNYAIWPSVLGASPKIVTTFGLLFF
                     KAAAVPMSMLMLFLIYWLLPNRKIPVADVLPAAVLVGLLLEGLKYLNILTWPLWRAKL
                     AREYGPFVYSVTIVLWSFLAAMIVLAGAEWCARSHRESPDAGEAAL"
     gene            50203..>50886
                     /locus_tag="VLE22_21650"
     CDS             50203..>50886
                     /locus_tag="VLE22_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17070.1"
                     /translation="MFRPVAPVCSAILVVAAFVSQPALAQEPTVDAILERYVQALGGK
                     ANLEKLTSRLATGIFEVPEWETKGPIEVYSKAPNKTMNTTDFAGYGVVARGFNGTSGW
                     SKDPDEGLRDVSGGELAEMKRAAEFYRELKLREQYAKMKLAGAQKLAGQDVQVIEATT
                     PEGLLHKLYFDTQSGLLVRHDMQSEIPGGKMTTVVMLGDYRDVDGVKVPFTIEQNNPN
                     FGFILRLKEV"
BASE COUNT         9805 a        16171 c        15171 g         9739 t
ORIGIN      
        1 ggtgcgtcag cccggcggca acaccttgac gatgtcgttg taaatcacga agaccattat
       61 tcccaacagg aagacaaaac cgagcttgaa aaccgcttcc ttgatgggca gactaaggtc
      121 tcttcgcatc accatttcga ccaacaacat caggatcacg ccaccgtcta gaacggggat
      181 gggcaggagg ttgaggatgg cgagattcag actgaccgcc gccatcaaca taatgaagga
      241 ataggctcct tggcgtgcgg catcaccgga gagttgcgcg atccggatag gcccatccag
      301 ggtcttggcc gacaggcggc gctcgacgat gccccgaagg acttggtaga tcagtgttgc
      361 gcccctggtg ttgtggtgga cggattccgc cagcgcctcg acgaacggaa gccggctgaa
      421 gacgacccgt tgttcggggc cgacaccgat catccatcgt tccggtccgt cgatactcgc
      481 tttctccggc tggactgtca cccgctgacg gagtccgtcc cggatgaatt cgatttcgac
      541 gggcttgcca ccacaggccc ctacgacttc gcgcagtttg aactgggagc ggatcggctc
      601 accgttcgcg ctgaggagca gatcgccgcg gcgaaggccg gccttctccg ccggcatacc
      661 gggggaaact tcacccacct gaatttcggc ctgctcagcc caaccggcga acccgatgcc
      721 aagcttctcc agcaggaccg gggtcactgt acagtcgatg cggcgtccgt ctcgctcgat
      781 gacgaccttg atgggcctcc tggcgttcga cgcctcagcc actaagatgt cctgccacgt
      841 cggattctcg aggtcaccga tcttgacgat ccggtcgccg tccctaatcc cagccttcgc
      901 ggcggcggag tccggcagga catgcccaac ggtggcgctc agatcggact cgacgggctt
      961 cggatacttg aacatgaaca gaccggtcaa cagcgcgatc gccaggacga tgttcatcag
     1021 cggaccggcg aacgcgatag ccagccgttg ccacctgggc tttgccaaga agccccgcgg
     1081 gtcggtaaca ctctcgtctc cgggctgttc gcccgccatc tttacgtatc cgccgaagag
     1141 tatggcggaa aatcggaagt cggtctcgcc cttacgaaat ccgaaaatcc tcgggccgaa
     1201 accgaaactg aacgtctcca cgcggacgtc aaagaaacgc gctgcccagt agtggcccag
     1261 ttcgtgaacc aggatcatca ccccgatcag gaccagcagc caccagatat tctgaactac
     1321 tgccatataa gctagattgt tgccaccggg cgacgaacgc ctgctgcggc acccctctct
     1381 ctttcttcaa tgatactgcg tgcaactaaa cgtgactctc tatcgatctc gagtacttcg
     1441 gccactgaac ggggttcccg gactgccaca cgcgacagcg tctcttctac gacctccgca
     1501 attgccagaa acgtcgtttt cccatgcagg aacgcctcga cggcgatctc atcggccgca
     1561 ttcagcgtgc aggtcgcaga cccacccgcc gcctgggcct ggtaggcaag cttcaaaagc
     1621 gggaacttct cgaggtcagg agggctgaac tcccagcgcc gggccaccgt ccagtcgagc
     1681 cgcggaaccg gcgccggacc gcggccgggg tatgtcaaag cgtactggat cggcatgcgc
     1741 atgtccgtcg cggatacctg ggcgatgatg cttccatcga cgtactccac catcgcatga
     1801 acagtggact gcggatggat caccacgtcg atctctgacg gcgacaggcc gaagagccag
     1861 cacgcctcga tgacttcaaa gcccttattc atcaatgtag cagagtccag agtgatgcgt
     1921 gggcccatct tccaggtggg gtggttgagc gcctcaacgg gagtgaccga agcgagttgc
     1981 tcgcgcggcg tatcccggaa cggcccccct gaggcagtaa ggatcagacg cagcacttcg
     2041 ctccgcttgc ccgcccgcaa acattggtga gcgccgttgt gctcactgtc caccgggatc
     2101 aactcgctcc gcgcgtcgcg agcagcgtcc atcacgagtt ttccgctcgc tacgagaact
     2161 tccttgttcg cgagtccgac tttctttccc cggcggactg cttcgtgtgt tgcctccagt
     2221 ccggccaccc caacgatggc cgacatcagg aagtcaactt ccggcgcaat cgccgcccgc
     2281 acgctcgcct cgggtcccca tccgagttcc ggccactctg agcgcggatg tccggactca
     2341 gccagccgat tcctgaggtc atggaggacc ttttccgtcg ccaccacaac caccgcaggg
     2401 cggaattcgc gaatttgggg gattagaacg tccaggtttc ggccggcgac caaggcgaaa
     2461 acaccgtact cctcggagtt tcggcggacg acgtccagag tgctcgtgcc aattgatccg
     2521 gtcgacccga ggatggtcag tctcttcatc cgggaaagca ggcgcagact tgcaccctag
     2581 gcaatgatat cagaacagcc aggagtggga gatccgaaac acaggtttcc acgcatgtgg
     2641 aaatctcttt ttggggcgcc ggggaggatt gcttcagggg tctttattag aggttagtct
     2701 cgctttggtg ggagttgcct ccccggctta cttccaataa taggatatga tttctcggaa
     2761 tgcaagcgaa aacttcgaat tttgaaagcg agaccgtcag ccccgcttga cttccacctt
     2821 ggcccatgcc tctaacgtct tgattgcaga gcagaaatcc tcgtctccga ggccggaagc
     2881 gagagctgcc cggaacatct gttccgtcag agcggtcaac ggcaggggaa cattgagttc
     2941 ttgtccggat tcgagcatca acccgatgtc cttgtgcatc cacttcagcg ggaagttcgg
     3001 gctgaagttc cggtcgagga tgaatggagc tttatattcg atcaagccgc acttggcggc
     3061 gctgttcgac aggatgtcaa gcatgagctg gggttccaca ccagcctttg tgctgagtac
     3121 gattccttcg aaaaaagact caatgatgtt gctgagcaca agattctgcg tcagcttggc
     3181 gtgcagtccc atgcccggcc caccgcaata gtaaaggcgc ttgcccatcg gttcgaaata
     3241 agggcggacc cgctcgaaaa cttctttatc gccaccgacc atgaatgtaa gcgtgccgcc
     3301 ttccgccccc gggcgtgacc ccgtgcaagg cgcgtcgagc atgtgcaacc cgcgttctgc
     3361 aagttcggcg ctcacagtgc ggctgcggct tggcgaaatc gttccggcat cgacgaccac
     3421 ggtgcccggc ctggcacccg cggcgatgcc gttgtctccc aagatcacgc tctcggacat
     3481 cgatgtgttg ccaacgcaga ggaatacgca ctcggcgaac tcccctacct cccgcggcgt
     3541 gccgcaatac ttgcctttct cggcatcggc caactgtttc gccttctcgc tggtgtgcga
     3601 ccagagggca acctcgtggc ctgcacgaag aaggttccgt gccatcgggt agcccatgat
     3661 cccaagaccg agaaatccga gtttcgccaa gttcacctcc agaaattttc tgtttccgat
     3721 gctgtccgca gccgaatccg tcaattgtac gacgccccgg ctctatgcga caattgcgaa
     3781 ccaaatgaca tccgtcaggc cttccgtgct ggtgacaaag cgcatctacc cagaagccat
     3841 cgaattcctt cggtaccacg ccgacgtgga ctatgcggac tcggacgacg gactgccacc
     3901 ggccgaactt ctcagccggg cgcgaggcag acaagccgtc gtctgccaac tcactgacaa
     3961 gttcagcgcc caggtgatcg agcaactgga gggtgtccgg gtacttgcca atgtcgccgt
     4021 gggttttgac aacatcgaga tcccggcggc tacccgcaag ggaatcttcg tgacaaacac
     4081 accggacgta ctcaccgaga cgaccgccga tttcgccttt gcgctgctga tggccgccgc
     4141 gcgccgtgtc gtggaggcac atgcgttcgt tcactcgggg gagtggcgcc gctggacgat
     4201 tgacctcctg gtcggccaag acatacacgg caagaccctt ggcattttcg gaatgggccg
     4261 catcggccag gcagtcgccc gccgcgccag ggggttctcg atgcggattc tctatcacga
     4321 cccgcaacct gtatctgcga tggtcgagcg cgaactgggt ctcgagttcg tcccgaagca
     4381 aaaacttctc gccaaatccg atttcgtcag catccacgta ccgcttctcc cggagaccat
     4441 gcggctgatc ggcgccgccg agctccgcca gatgaaaccg acggccatcc ttgtgaacac
     4501 cgcgcgcggt ccggtagtcg atgaggctgc agtggccgaa gcgcttcggg cgggcatcat
     4561 cgcctccgcc gggctggacg tgttcgaacg cgagccggag gttcatccgc tccttctcga
     4621 gctgaaaaac gtggtgctgg ctccccatat cgcgagtgcc tctgtggaga cgcgacggcg
     4681 catgtcgatg atggcggcca acaacgcggt cgcgatcctc gaagggcgcc gccccccaaa
     4741 cctggtgaac acagagctgt ggccggacca agcggccggc ccagttcctt gatttgagcc
     4801 ggatgcaatt tttcaccacc tctttgtggg tctggtatac actctgagca ggagggtgta
     4861 tggcccacct caagcattcc actcatcccc tgcgccctgg cactgaggtc cttacgcgtg
     4921 gctttgatcc tagtctatcg gtcggttcag cccggcctgc gctgttccgg tgttcgacct
     4981 acgttttctc cagtcctgag gcggcggaac ggagtttcgc ggtgtctact gggcgtgctc
     5041 acgccgaagc aggcgagcac gtcgaactaa tctatgcgcg attgaaccac cccaacgccg
     5101 agatcttaga ggaccagatg gtccccctcg aaccgggcgc aaaggcggcg gcggttttca
     5161 actccggtct cgccgctatc atgacggcgt tccttacgtt cgcgcggccg ggagatacga
     5221 tcgtttatac ggtgccgctg tatggcggaa cgcagcacct gattcacgaa ctgctggagc
     5281 cctttggcgt cacttgcgtc gcggtgccgg ccggaaacgg gttcgaactg gacgaggcca
     5341 tccgggcggc gagaaacctg cgcatcgtct tcatcgagac tccggccaac ccgacgatga
     5401 tgatgacgga tatccgccaa gcggccaccg ccgcctccct gcgcggtgac ggcgtggtcg
     5461 tgatggtcga caacacgttt cttgggccga ccttccaaca ccccctctct gttggcgcag
     5521 acatggtcct gtactcagcg accaagtatc tctcgggttt cagcgacctc atcgccggag
     5581 ccgccctggc cggcgacccg gatctgattc gcaagatgcg gctccggcgc gcgatcttcg
     5641 gcaacatcct gccgccggac gagtgctggc tgcttgacgg caggctgcct acggtcacgc
     5701 tgcggatgaa ccgacagagt aagaacgcgc aacggattgc cgagcgtttg gcggtacatc
     5761 caaaagtgag gaaagttcac tatccgacgc tcttcgacga ctcggaacag gtcagaatcc
     5821 ggctggcgca gtgcgcgtat cccggcgccc tcttttcgat gcagttgcgc ggcggaaaac
     5881 cggcggcttt cgactttctc aggaatctca agatcgcccg gaacgctgta tctctaggcg
     5941 gcgtggagac gctcgcgtgc cacccgatga ccacaactca ctcagggctt acgccagaag
     6001 aacaccggct ggccgggatc accgacggac tcgtgcgaat ctcagtcggc attgaagact
     6061 ggcgcgatct gctcgaagac ttcgagcaag ccctggaagg gctatagaac cgcctacacg
     6121 aacaacatcc gcgtggttgt attgactagc gcgtgcgtga ccatggcggc acggatgctg
     6181 ccggctctgc tgaaagccct tccatagaag cagccggcga ttgtcgcgac caacgcaaat
     6241 ctccagttcg ggaactgacg aaacggcaga tgcgccagtc cgaacaggac ggaagcgatc
     6301 aggacagccg cagtctcgtt ggccagcagg cggcttaact ggtgctgcag gacgcctcga
     6361 aagaaaaact cctcggagag tgccactacc caaagcatcc cgaaaaaagt cgccgcggca
     6421 agcggcgtga ctttccacca ctcgagaggg actggacgaa agcgaaccag acccgttccg
     6481 agtgcgagtg gtattccgac cgggaggaaa tatacgtagt ggcgcaggcc gatcccccaa
     6541 tccccgcgca gcggtacgaa gccaaaaccg gttccctcga tccggcgcag gatgagcatc
     6601 gcaccaactc ccgtgcgaat ccacatcaac tgcccgagga tgtcgatccg gatgcgcggc
     6661 gccgggctcg gatagagatc ggggaagacc ttccacaaca cgatgccggc gaggaaggcg
     6721 agaaacgcga aatcggaagc cggcttgtgc ggcattgcca ggtaccaccc ggcggccact
     6781 gtcgcgagca aaacgattcc ggtgatcgcg cttacgcgaa actgccctgt cggaagactg
     6841 tagatcaggt atggcactac ggcgcccaca acgagcccgg ctgcaatccg gggcgccgaa
     6901 tagcgggccg ccagcgtctc gcggaactcg cggaatgcag gcgcgaaata gagcgaaatc
     6961 tcgaccagga gagcaaacag caggggaatg gcgataccga caggtatact caggaatcga
     7021 gagtagatga cgccgcccgc tcccagaaca agccagatgc agatcagaac ggcggcgtat
     7081 cggcggagcg cggcctgcac tcggttcatc ttagcactcg tctggctggc tagcctggcg
     7141 gcagctcaag cgccgccgtc cgacctagcc cggcgcgtgg cggaacgcga ggcctccaac
     7201 gagcaggaac ggcagaacta cacctaccgg cagacagtgg tcctcgagga actgacgccg
     7261 tccggcgcga gatcgggcga gtaccgcgaa gtccgggaca ttatcttctc tccggaagga
     7321 gagaggaccg agagaatcga cgggcaacca cggaatcttc tgaggagcct ccgcatgacc
     7381 gccgaggatt tccgggatat ccgggaggtg cagcccctgc tactcacgcc cgaccgcctc
     7441 tggatctacg aaacgcgcta tcgcggcgag gaaacggtag acgaaacaga ctgctgggtg
     7501 ctccagatcc gcccccgtca aatcctcgat gggcagcgcc tgttcgacgg tctgctctgg
     7561 gtgaatcgtt tggactattc cattgtccgc atggaaggtc aggcggtacc gcaaatcctt
     7621 catacgagcc gcgagaacct ctttccccga ttcacgacgc tacgccgggc gttcgagggc
     7681 aagtactggt ttccagtcca cacgcacgcc gacgacaccc ttcccttccg ttcgggcgcg
     7741 caaagaatcc ggatgacgat tcgctacgag aactaccaac gcttcgccgc cgaatcgacg
     7801 gtccgcttcg agaaaaaata aaacggccgc ccggtgaggg gcggccgtcg acaaacgaag
     7861 gctgattgcc ggaagcgctc agcggtctag tagtccatct ccgggccgtg gccgtgcggc
     7921 atgggcttat ctttcttctc tgggatctcg caaacgagag cctcggtggt aagcatcaga
     7981 gccgcgatcg acgaggcatt ctgcaacgcc gtccgcgcca ctttggtcgg gtcgatgacg
     8041 ccggaggcca ccaagtcttc atactcgccg gtggcggcgt tgaacccgaa ggtgggtttg
     8101 ccgttgttgc ggaccttctc gacgacgatc gcaccctcga agcccgcgtt gccggagatc
     8161 tggcgaatcg gctcctcgca ggagcgcttt acgatgtcca cgccgatctg ctcgtcgccg
     8221 gaaagcttca ggctggcgag agccgacgcg gcacgcagca gcgccactcc gccgccggga
     8281 acgataccct cttcgaccgc ggcgcgggtt gcgtgcagtg cgtcttcgac gcgggccttc
     8341 ttctccttca tctcggtctc agtggctgca ccaaccttga tcaccgcaac gccgccggcg
     8401 agcttggcca gccgctcctg aagcttctcg cggtcgtagt ccgacgttgt ctcgtcgatc
     8461 tgtgcgcgca actgcttgat ccggccctcg atcgccttct cgctgccggc gccctcaacg
     8521 atggtcgtgt tgtccttgtc caccgtcacc cttttggcgc ggccgaggtc atccagccgg
     8581 acaccctcga gtttgatgcc agtctcttcc atgatcgcct tgccgcccgt gagaattgcg
     8641 atgtcctcga gcatggcctt ccggcggtct ccgaaaccgg gcgctttcac cgcgcaggcg
     8701 ttcagcgtgc cacggagctt gttcaccaca agcgtcgcca gggcctcgcc ttccacttcc
     8761 tcggcgacca cgagcagcgg cttgccggag cgcgcgatct gctccagaag tggcagcagg
     8821 tccttcatgt tgctgatttt cttctcgtgg atcaggatgt agggatcctc aaccacgcac
     8881 tccatccgat ccgggtcagt gatgaagtag ggggagaggt agccgcggtc aaactgcatg
     8941 ccctcgacca tgtcggtggt ggtttccatg gtcttggact cttcgaccgt gatgacgccg
     9001 tccttgccga ctttcttcat cgactcggcg atccgatctc cgatcgtcgt gtcgccattg
     9061 gcggagattg tgccgacttg cgcgatcatc tcgccgctga ccggcttgct gtacttcttg
     9121 atctcctcga caaccgtctc gacggcctgt tcgattccgc gcttgagcgc catcgggttg
     9181 gcgcctgccg caacggcctt cacgccttcc cggaaaatcg actgggccag gatggtagcc
     9241 gtcgttgtgc catcgccggc gacgtcggag gtcttggacg caacctcacg aaccatctgc
     9301 gcgcccatgt tctcgagcgg atccttcagc tcgatctctt tcgccacggt gacgccgtcc
     9361 ttggtgatcg tggggccgcc gaatttcttt tcgagaacga cgttccggcc cttcgggccg
     9421 agagtgacct tcaccgcgtc ggccaactgg ttcacgccgc gcaggatcgc ctgccgggaa
     9481 ctctcgctat agacaatctg tttcgctgcc attttccttc ctcctgaatc ctaacctttg
     9541 gcctggtcga tcactccgag aacctcgtcc tcgcgcatga tcaggtattc ttccgcgtcc
     9601 agcttgattt cgctgccgga gtacttgcca aatagaatcc tgtcgcccac cttaacgtcg
     9661 agcgggacga gcttgccgtc atccaggcgc ttaccctgtc caacggcgac cacctcaccc
     9721 tcctgaggct tctctttcgc ggtgtccggg atgatgatcc cacccatcac cgtttccttc
     9781 tcttcgatcc gtttgaccat gatgcggtcg taaagcggcc gaatcttcat tggacaaaac
     9841 cctccatttg aactcttttg ctagagattg aagcggaaac ccggcgaaca ccgggtccag
     9901 aggtaattat tagcacactc cggggaggag tgacaagagg tttctgtaac atattgaaat
     9961 tgaatcgttt gtttgtctac ggatttcacc gtttgggtca gtgtgtttcg caccttgcag
    10021 cggggcgttt cggaattgtc aaatctggta gattggtttt gaatgcggcg tacccgcccg
    10081 cttctgctgc tggccatcct cgccgtcctg tgcgccgttg gcgttgcgtt ctatgtgcag
    10141 aaacggtttc agctagcgag tacggcgccc acgccgaagt ccttgcctcc aggaacaagc
    10201 gccaccgcgg aatactggag ttggtctcac acccaagagg ggcggtctgt cgtggaggta
    10261 cgggccgcga actaccgtca gatccgcgag ccttcccgct ttgatctcga aaaggtcgaa
    10321 ctacggattt tcgacaagga tggtaagcgc tacgaccagg tgagcagcgc ccatgcccag
    10381 ttcgatatcg aggcgggcgt gttgtattcg gatggccagg tcgacatcac gatgggagta
    10441 cccgccgaac ccgctcctgc ggggcgactg gtcttcattc gcacatcagg agtcaccttc
    10501 gagagtaaaa cgggcagagc gttcaccgac cgcctggcgg aattcacctt cgatcagggc
    10561 acaggcaagt gtgtgggtgc gtcgtataac ccggaattcc gcgagcttca catgcgctca
    10621 caggccgaag tcaattggcg cgggcggaca cccggctcga ttccgatgaa gatagaggcc
    10681 ggcgagttga tttacaagga gcgcgacgca gccgtcctcc tgtcgccgtg gtcgcgattg
    10741 acccgcggcg gcacgctgct cgaagccggc tcttccatcg tctggctggg ggaagaagcg
    10801 atccgccgtg tggaggctaa ggatgcgcac ggcagcgacc ggagtgagga ccgccggatc
    10861 gagtacgcag cggcagtgtt gtacatgacc tttgcggcgt ccagccaggt agagcgcatc
    10921 gagggcgagg ggaatgccaa actttcatcg atctcggcga caacgcgaac tgaggtgtcg
    10981 tccgaccggc ttgatctgga attcgacgcc actcgcagcg acagtgtcct caagagggcg
    11041 tgggcaaaag gccgcagctc ggtgcagtcg acgccggttt cgaccggttc atcgaaaccc
    11101 gccgttaccc gaacattgcg cagcgacgtg atcgagttga caatggcgcc gggaggccgc
    11161 gagatcgctt ccgtcttgac gcattcgccg ggcaccgtcg aattcgtccc ggaccaagcg
    11221 gctcagaagc aaaggaaact cgacgcgggg cagatccgta ttgaatacgc aggcggcaac
    11281 cggattcgga actttcacgc gacagatgtc gccacgtgca ccgaaactcc ggcgatcgcc
    11341 ggtggcaaga aggccccgcc tccgatgctg acctggagca aggagctgac cgctgagttt
    11401 gatcagcaga gtggcaggct ggcgcggctg gggcaatcgg gcgactttcg ttacgaagag
    11461 ggagaacggc gaggccgcgc ggaccgcgcc gtgctcgaag cggccagcaa tctgatcact
    11521 ctggagggct cagcccggct ttgggatgcg gcgggatcga cctcgtcgga tcgcatcctg
    11581 ctcgaccagg cgagcggcga catgaccgcg gtcggcaagg ttgcatcaac gcgagcgccc
    11641 gagagccgga ctggttcggc gggtcttata tcgtcaggtc agccgttgca ggcacgggca
    11701 gaccggatgt ccaccaccgg ccaaaaccgc cgcattcgct acgaggggga cgccgtggca
    11761 tggcagggag ggaaccgcat ccgcgccgat gtcatcgaaa tcgaccgcac ggggcgcgtg
    11821 ctcaccgcga acggaagcgt ggtcagccag ttcctcgatg agccttccgg cgccgaatcg
    11881 gataggaaac cggctcgaag tccagggttc acggtcgttc gggccaaaga gttgcgatac
    11941 gaggacgaaa cccgcattgc gcactatcgt ggcgacgtca agatgactcg caccgccacc
    12001 gaggtgaaag ccgcggaact tcgcgcacaa ctcgctgccg gtgattccgg ttcgcgcctg
    12061 gagaaagcgc ttgcggatgg ccgagtcgag attctccaca gcgcgccagg acggaaccgg
    12121 agggccaccg gcgaacacgc tgagtattac ccaggagaag agaagatctt gctgtatggc
    12181 ggatctccgc tgctcgtgga cagccagcgc ggctccacga aaggcaatca attgacctgg
    12241 tttgccaaca atgataggct gctcgtggat ggccgggagg atcagcccgc catgagccgc
    12301 atccttcgca agtagcccga atgcgaaaac tccagacctg cgaaatctcc aagtcttacg
    12361 gcggccggaa agtggtggac gatgtgtcga tcctcgtcga gcagggcgaa gtggtcggcc
    12421 tgctcggacc gaatggggcc ggaaagacga cgtcgttcta cacgattgtc ggcttgatca
    12481 gccctgattc cggccgggtc atgctcgacg accaggagat cacccaactg gccatgtatc
    12541 agcgggcccg acgagggatc agttacctgc cgcaggagcc gtccgtgttt cggaagctca
    12601 cggtcgagga aaacctgatg gccatcctcg agacactgcc ggtgagtgtg cacgagcgcc
    12661 gggagaggct ggcgagcctc gtggagcagc ttggcctcga gaaggtgaag cagaacaagg
    12721 ggtacgcgct ttcgggcggc gaacggcgcc gggttgagat cgcacgttcg ctggtcatcg
    12781 acccgacctt cctcctgctc gacgagccgt tctcggggat cgacccgatt caggttctgg
    12841 aactccagcg catcattttc gatctgaaac ggaccggaat cggcatcctt gtgaccgacc
    12901 acaacgtccg agagacgctg gccgtgaccg accgggccta catcatcaac aacgggaaga
    12961 tcttccgcgc gggtagtccg gaagccctcg gaagcgatcc tgaagtgaag cgagtctacc
    13021 tcggcgaagc cttcaacctc ggggtcgcga cccagtcgta gcccggcttt ccgggcaccc
    13081 gctgtactcg tctgcgctac aatgagggcg aatgagtttg ctttcgccac ggctgcaatt
    13141 gaaggtccag cagaagcaaa tcctgacccc tggactcgtc cagatggtca ctgtcctcca
    13201 attgaaccgc ctggaattaa aggatctcat cacccaagag atctcccaga accccgtgct
    13261 ggaagaggcg gtcgacgacg gcacggaaga attggcgccg gaggaagtgc aggccattct
    13321 ggagacggag cgggacgcgg cggatccggc ccccgccgac caaggaatcc tcgaggtcgt
    13381 caatacggcc gcggagttga ccgagggagt ccccgaaaca gaggtggaag ccatcgacag
    13441 ccgtgtggcc ccggcgggga acgaacccac aacggagacg gtcgaggccg tcgaggcgca
    13501 agcctcaacc gatccgttcg aagaaatcga ttttggttca tttttcgatg actacctcga
    13561 ccccggttac aagagtcctg cctccgagca ggtagataag ccctctttcg aaacgttcct
    13621 ctcggcgccg gtgacgctgt ccgatcacct cgattcgcaa ctcagcctct tggtcctctc
    13681 ggattcactt cgcgaagcgg cggaattgat tatcggcaat ctcgatgaga acggttatct
    13741 gacggtctca ctcgaagaga tagccggctc ctgcgagcat accgctgagg agctcgacga
    13801 ggccttgcgg attgttcagt ccctggaccc agccggcgtc ggggcccggg atgttcgcga
    13861 gtgcctgttg ctccaactgg aaagccagaa cggccggggt ggtgtggctt ggacgatgat
    13921 ctccgagcat ctaaagctgg tcgagtcaaa acaattcaag gaactggcgc gactgctcgg
    13981 ccgtccgatg gaacacgtcg agattgcgga ccacgtgatc cgcaacctcg atcccagacc
    14041 cggcctgcgt tactccggcc ccggcgcgcg tcagatcgag cccgacgtct acatctcgaa
    14101 agagggtgaa gagtacctca ttcagttgaa cgacgaggat ctgccgcaac tgcgcttgaa
    14161 ccgccagtac cgccggatgc tggatcgcga tcaggctccc aacaaggacg tccgcaatta
    14221 cgtcaaggag cgatacgcgt cggcgatcca gttgatgaag aacatcgaac agcggaagca
    14281 gaccatcctc aaggtttgcc agtccatcgt gcggcgccag acggacttcc tggaccaggg
    14341 aatcgacagc ctgaagccga tgatgatcaa ggatgtggcc gaggagatcg gtgtccatcc
    14401 gtcgacggtc agccgcgcgg tcgccaataa gtacgctcat accccgcagg gggtcttcga
    14461 actgcgatac ttcttctccg aggcggtcca gggaccctcg gggagtgcga caccgctttt
    14521 gattctgaag cgccgggtga agaagatgat cggggaagag gatccaaccc atcctctaac
    14581 cgacgagcag atcaccgccc gattgcaggg cgagggcatc cacgtcacac ggcgtaccgt
    14641 ggccaagtat cgagaagaca tgaatattcc gtccacgcac cacaggcgcg tgcgaagcta
    14701 aagtccggac tgccggaggg tcggatgaaa gtcacatata ccggtcgcac tgaaaaactc
    14761 acccccaccc tacagaagaa gttggacacc aagttcgcca agctcgccaa actactcgac
    14821 caaaagggtg gcgagcggga agcgcacgtc attctgaccg tcgagcggca ccagcagcag
    14881 gccgaaatca ccgtccagtt ccacgaccac ccgctggttg gaattggcgc gagttcggac
    14941 cagttcaccg ccatcgttac cgccgccgac aaacttgaga aacaggttct caagctgcgg
    15001 accaaatggc gagacaccaa gcgcggaccg aaggaatcct ggactgtacc agccgctccg
    15061 gaggcggaag aggccgccac gccggagcct gccgcagaga agcgcatttt ccgcgtcaac
    15121 cagcacgcgc gccggaagcc aatgaccttg gacgaggcca tcctcgagat ggaagagaat
    15181 cgcgactacc ttgtctaccg cgacgcggaa accgaccgac tctcggtgct cctccgccgg
    15241 cgcgacggcc actacgactt ggtcgaagcc tgagtccacg attcggatgc cgactaggag
    15301 cgcgtcccgg caggtcaacc gggagccgga gcttgtcatc attaccgggt taagcggatc
    15361 cgggaaggtc accgtgctca agtgcatgga ggaccttggc tactactccg tggacaacct
    15421 cccggtagat ctcatccaga aattcggcga actggtgaag gattcaccgc acattcgccg
    15481 cgccgcgctg ctggttgaca ttcgcgaggg cgaggctctc aagcggtttc ccgaccttta
    15541 tgcgagaatc cgccgggagc gcccctgcac cctggtgttt ttcgaggccg acgaggccac
    15601 gcttctacgt cgctacagcg aaacgcgccg gccacaccct ttgggaaccg aggaatccgt
    15661 ggtgcgaagc cttcgccggg aacggcgtca gttagcgccg atccgggcgc tcgccgagca
    15721 catcatcaac acttcgcgat tcaacgtcca tgagctgcgg gaattcatcg cggagaagtt
    15781 cggtggaaac cgtgaccagt cgcgcatcat ggtcaacgtc accagtttcg gctaccggta
    15841 cggcgtgcca ccggatagtg acctggtgtt cgacgttcgc ttcctgccta atccgaacta
    15901 catcccacaa ttcaataagc tcagtgggcg gcatccgagg gtggcccgct acatccgatc
    15961 gttcccacaa acaacggagt tcgtccaacg gatcacggac cttctcgtct atcttctgcc
    16021 gcattacacc cgtgagggca agagttacct gacgatcgcc tttggctgca ccggcggccg
    16081 ccaccgctcg gtgatgatgg ccgacgagat ccgccgtcgc ctttctcgcg ttggcttccg
    16141 cgtcaagtca acgcaccggg acatcgagaa gagtggaacg tagttggcga gccggggcgc
    16201 ccacgcggaa aatctaccgg tttcccaatg tccctgattc gtttgacctt agcgcggccg
    16261 gtgcggtatc ctcaacagaa gagtccgatc gcaaggagta gacagtaact agatggcaaa
    16321 cactttttct gccttgaagc gggtgcggca gaccgggcgc aaaaccgaaa ttaaccgcgc
    16381 gcgcaagtcc aggctgcgtc accagatccg taccttccgg cgcctgttgg agacgaagga
    16441 catgagcgcg gccaacgccg cactgcccgg tactttttcg atcatcgacc gcgcggcgaa
    16501 gtggggcatt attaagaaga acacggcggc ccggtacaag tcgagactaa ccacccgcct
    16561 gaaggcgacc gcagccgcgt aaggcccttc actccgccag tctcagaata aattcctcca
    16621 gcacgatccg gtcgtcgggg ttggcgtccc gaagagcact gtccgtttcg aaaatcttct
    16681 tcagcaccgc ctccagttgt tcccgagtga agatggacgc ggtgtgcaga atttgctccg
    16741 ctcgagaccg ccatatcggc cgccccggac cggaaagctg ctgttgcacc tgctggggag
    16801 tgcgaagtcc cttttccttt gcggccagcg caagacggaa cagcgttgcc agaaagctca
    16861 acgccagggg gagatactct ccctgacgaa cgagcttgtg cagcagctcg agagcgcgca
    16921 tacggtcccg acggcccaag gcatccacga gcacaaagat cgtcgatgcg ctcgagtccg
    16981 gcacgagtgc cgccacgtct tccgcctgga ccttcctcga tcggccggtg tacaaccgga
    17041 gcttctcgat ctcggccgca acccgcattc cgtccgcgcc tacggtttcc acgagcgtgt
    17101 cgatctcggc gcgatcgatc gttacgcccg catgccgagc cagttcacgg gcgagcagcc
    17161 tcgcttcctg gacggtaagc ttgggaaact cgaccactgc gggcacgcag tcgtaaaact
    17221 tacgcacgcg ttctgctttc tgcttgtctt cgccttcaag gtcgtaccgg ccagcttcca
    17281 gcactatgac cgagaggggt gtcggatccc ggacatacgc ggcgagcgtc tccgccgccc
    17341 gcgccgttcc gcctttctcg tcctctccgc cggctttgcc ccgcggaaga gcggcctcgg
    17401 cgtttgaggc gcagatcacc ctgcgtgggg cgaacagcga gagcgaccgg gcatcatcca
    17461 ctacatcgct caggcttgtc tcatcgaggt cgtagtgcga aagtccctcc tggcgctcct
    17521 caggactcag cacacgttcc accagcgccc tccggcactg gtcccgccga tgcggttccg
    17581 cacccaagaa caggtagacg ggcgcgggtt cctgccgctg gatctgagca aggaattggt
    17641 ccggtgacat gttcaaaatt tctcaagaac tgcactgacc accgtgcggg ctacgtcacg
    17701 gcttagccgg tccaacgcgg gatcactctc ctcgaaatat gccacctggt cgacgctgat
    17761 ctcatagcgt tccctggctt cgaggccgct tcgtgcgtag agcacagcgc ccgtggcccg
    17821 ttcacgcagc gaaatgtcga gaactacgct catctgtacc gcactggcac ggctcgtcgc
    17881 cggatcaagg attgtcgggt agtagttata gagagccacc cgcccttcga gcacggcgtc
    17941 cgcttcggcg gggtctgcga ctatccggta gcgagtgcgc gagagaaact cccgggtgat
    18001 cgcggaaggg agccgttctg tcagcttgta tcgaatcgtc gcattggtga acgcgggaac
    18061 agcgatcgtc ttgatgttct ttggcaggag gtccgctttt cccgcgacgt ggtatccgca
    18121 tccgcccagc cataatgcag ggaggacagc cacgaccaac cagggacgca ggatctttcg
    18181 tctcattgtt ccccgcgctg ttccgtacgc agcagggaag ctgccacggc cgtcgcattc
    18241 tcggccatca gccggaagtt gtcggcgaca atccagaacc aaaggcctcg cgggttattt
    18301 cggtcccgtg cgatggagcc cacggcaatg ccgccctggc cggcaatccc gacattcgtt
    18361 ggcggctcga catcaacccc acggacgtcc acttcggggg acgccagcgc ggtggcaatg
    18421 gcttctgtgt cgggcgggtc ctcgaactcc gcgtgaacgg aaatgctgtg gccatgaaag
    18481 acaggcgcct gaatcaagcg gatcgagggc atggggacgc cgcgaagccc cgcgaggaga
    18541 gtcgccaagt gccgctctat tcgctgctca atcgaaccca gagcttccgg ggcttcactc
    18601 ccatagcgcg aaagcatcgc gaagcttagt tgtgtgtcaa aaacgtccgt cggcagagtc
    18661 cggaaggaga gaagactgac cgtctgtttc tgcagttcgt cgagaccctg ctgccctctt
    18721 tcgctcgccg gctcgaagat atgagcgatg acgttcagca acttgggcgt tccggcaagc
    18781 cggctcaaga atagggaaag agctatcgcc gccggatgcg cgatcacatg gagtgttcct
    18841 ggtggcgggg catatccagg cggctcggcc acaggtgctc gcaatcgcgc cgacggatcc
    18901 tcttccgtcg cgtaggtgag atcgatgata gcgccttgcc gcgtggactt ccgggtcagt
    18961 tccatggctt tccggctcga atcgggtgag ccggcaagaa acgtcacttg cgcgtccctc
    19021 aggttggtct cgtcgagcgg cgtgatcacc gccgcttcgc ctccctcaga cgtcagcttg
    19081 gcggtgtctg cgtccacgcc gatcaacttc accgagcggg cgattttctg ctcgttgatc
    19141 agttcgcgca gttcccggcc gatcagtgac tctccaccca caattgctac agttcctgtc
    19201 ttcatgcgac tagcccggca aactctccgt acgtgggacg ggatggcgaa gccgcctctt
    19261 aacaatgcca ccgagccgaa cgacaattcc gctacccaca tacgtgaccg ccatcgccaa
    19321 cagcacgggc tcgggaaaat tccagatcag gtagaaaaat ccgccccaga caatcaccgt
    19381 caacggagac cgcggcgcca acagttgcac gtccttcatg ctcctgtacc gccacgtgct
    19441 caccatcaag aacgccagga gggccatcag caccagccac gcgagcgcca gcggccagta
    19501 ctcgatgggc gctgaatccg cggcaaacac aatcgcggcg acgagcgcgg cagccgccgg
    19561 gatcggcagg cctacgaagt actttctgtc cggcttcccc ggattctttg ggactgggtt
    19621 cttctggacg ttgaaccgcg ccagcctgac ggctccacag agcaggaaaa ggaaggcgat
    19681 gaagtagccc atctgccgca ggtgatcggc cacgaacgcc cccgtcgtcc catccacgaa
    19741 ctggacaccc caggagaccg ccagaacggc cggcgcgact ccgaaggtga tcacgtcggc
    19801 aagcgagtcc atttcacggc cgaaatcgct ggttgtattc gtcatgcgcg cgatccggcc
    19861 atcgagtccg tcgagaaaaa ccgcgacgcc gatcgccttg gcagccactt cgaagtgagg
    19921 actgtgcccg agttgtccgg aggcggcgag aagcgcaccc tggaatgact tgaggatcgc
    19981 gaggaacccg agcagagcgt tccctgccgt gaagagcgtc gggagcgcat aagcggctct
    20041 ccgcggccgc cggtccggag aattcggatc gatcagtctc tcgggaaggc tcagtctgcc
    20101 catcctggct ccagtccctc cggcgtcatc cgctcgcccg cgcgcaatgc gggcgcagcg
    20161 aattcgcaac gccacgcgag cacactcgat ccccccatga cgcgctctcc ttctcgcact
    20221 tggattgccc attccgggcc gagcacgacg tccacgcgtg atccgaactt gatcaggcca
    20281 acccgttcac ccgtctggac gacatcgccg gttttcttct tgaaaacgac acggcgtgcg
    20341 atcagccccg cgatctgctt gaaaacgaca cgtgttccgt ctccctggat cgtgacctcg
    20401 ttctgttcgt tctgggaaga ggcttcctcc cggctggcaa ccaggaatcg tccgggcttg
    20461 tattgaacgt ccgtaatgca tcccgaaatc ggcgcccggt tgacgtgcac gtcgaagata
    20521 ttcaggaaga tactgatccg tgtgtgcgac ggaccctctt tctttacagc cacgatcttg
    20581 ccgtccgccg gagacacagc caccgctcca gccggaatcg ggcgctcggg atcacggaag
    20641 aagtaaaggc aaaaggctgc aagaagatag agcgggatgc cccatgcagg gcgagtgaag
    20701 tacgagacca cgccgccgcc ggcggtcagt gcaagtgcgt agtagattcc gtcgagcacc
    20761 atcgactgcc accattctcg cacaacgcca gcagcacgga cccgggtcct cggtgagctt
    20821 aactacaacg cagccacgag cttcgcgagg agggcggtcc ggtcggcgat gcggtccgcg
    20881 aggatgtttt cgcgtggcga gtgggcgcct tccccgaccg cgcccagccc gtcgagggtc
    20941 gggactccca aggccgcggt gaagttgccg tccgagccgc caccgctcgt ggattcttca
    21001 agctcgcgtc ccagttcgcc cgccagccgg cgcgccgtcc gaaatagacg aacaacactg
    21061 gcggaccgct ccatgggcgg acggttcacg ccacccgcca cctcgatgat gcatcgcttg
    21121 tccacgggct tcagggcccg gaatttccga tcgagggccg cggcatcgct cgcccgcggc
    21181 gcccggatgt cgaacgtgac ccgggcctcg tccggcacca cgttcgacct cgtgccgcct
    21241 gaaatcacac ccggactcac tgtgacaccg cgctccaact tcgtgaatct ggagaccttc
    21301 ccgatctgtt gagccagttc cacgattgcg ttggcgcccg aggagaaatc cagaccggcg
    21361 tgcgccgacc ggcctcggac gatcatcgtg taatcgccga caccctttcg cgcggttttc
    21421 agctttcctt cgagcccggt ggcgggctcg agcaccagga ccgcttcgct cttacgagct
    21481 tcccgctcga ttagagcacg ggaagagtcg cttcccacct cctcgtccga gttcaccaac
    21541 agtacgactc tgtggctcac cggcgtctct agtgcccgca acgcccgtat cgcgaaaacg
    21601 aagaaggcaa gccccccctt catgtccagt gcgcccgggc cccagatcct cccatccgcc
    21661 tcccggaacg gcattcgggc aagcgtgccg gtcgtgtaaa ccgtatccga gtggccgatc
    21721 gcaagaatct ggccggactt tcgacgcccg ggcagctcga attcacagcg aaggtggttt
    21781 ccgaatcgcc ccccgggaaa tgttctggtt tccgcgatgt ccgagacggc gccggcaaac
    21841 agttcaacga aacaatttac ggctgccgga tcgtcactcg gcgactcgca ctcgacgagg
    21901 cgccggatga acccgacgat ctccgactgc cgcgactgcg caaaagagag gtggctgtcc
    21961 atcgcttgct ataatagcca gtccacatgc cagtcctcga cattgaagcc atccgcaaca
    22021 tcctgccgca ccgttatccg atgctcctgg tggaccgcat cctcgagttg gagccggagc
    22081 ggatcgtcgg aatcaagaac gtcactgtca acgaaccgtt ctttgccggc cacttcccgg
    22141 cgtttcccgt gatgcccggt gtcctgatcg tcgaggccat ggcgcaggtg gcgggcgtgc
    22201 tcgtgctgaa ggagatcggt gaccggcaag acaaacttgt cctgctcgcc tcgatcgaga
    22261 aggcgaaatt ccgcaggccg gtgttgccgg gagaccagtt gcgtatcgag atgaaggtcg
    22321 gcaaacggaa ggccagcgtg gcgctgatgt acggagaagc caccgttgac ggggtggtgg
    22381 tggcggaggc cgaggtgctc tgtaaactgg ccgaccgcga gcagaaaaag aacccctgat
    22441 tcagcccgaa tgccagttca tcccacggcg atcatccatc cccaagcccg ggttgcggag
    22501 agcgccgata ttggtcccta ctgcgttatc ggagcggaag tctcgatcgg cgccgggacc
    22561 cggctcatgg ctcacgttta catggaaggg ccgaccgcca tcggcgaaga caacacgttc
    22621 ttcccgtatt ccacggtcgg cgtcgcttct caggacctga agtacaaggg cgagcgggcg
    22681 gagacgcgcg tcggcaaccg caaccggatt cgcgagtttg tgaccatcca ccgcggcacc
    22741 gaaggtggag gcctgctgac cgaaatcggc gacgacaacc tgctgatggc ttacgtccac
    22801 atcgcccatg atgtgcgcgt cggcaaccag accgtgctgg ccaacggcgt cacggcggga
    22861 gggcacgtcc tgattggtga ctgggcagtc gtcggagcgt tcacgggatt gcaccagttc
    22921 tgccgagtgg gccggcactc gatgatcggc ggatacagcg tcatcaccca ggacgtgatg
    22981 ccgttcgcaa cgatcgtgag cgaccgtgaa acgaaggtgt tcggcgcgaa caaaaccggt
    23041 ctcgaacgac ggggctttcc cgacgcctcg atcgacgcga tcaacaaagc catccggctg
    23101 ctgacccgat cgggcttgaa tgtttctcag gctatcgatc gtattcagtc ggaaatgccg
    23161 gcgtgttcgg aagtcgaaga gatgctggag ttcatccggg gctccgagcg cggtgtaatc
    23221 aggtagaact ccttgtcgtt cccatgaagt acgctctcat cgcgggcaac ggccgctttc
    23281 cgatcctcgc cctcgaggaa gccggaaagc tgggtcacga ggtcgtcgcc atcggcattc
    23341 gtgaagaagc ggcacccgag atcgaaacgc tggcgtcgcg ctgcttttgg atctctctag
    23401 gccagttgag ccggctgatc gagatcctca agagcgaagg tgtcagcgaa gtcgtcatgg
    23461 ccggtcaggt caagcacgcg cagatctttt cttcgatccg cccggactgg cgactgctca
    23521 agctgctgac atcgctgcgt cagaagaata ccgatgccct gatcggcggt gttgcgaaag
    23581 tcctcgaaga cgagggcata cgcctggttg attcgacctt cctcctcaag ccgttactgg
    23641 cgggggaggg ggtgctgtcg cggcgcaagc ccgaggacga cgaagaagcc gacctcgcat
    23701 acggccgccg cgtgggcaat gcattggccg atttcgacgt cggccagagc gtcgccgtag
    23761 ccgagcgcgc ctgtgtcgcc gtcgaggcga tggagggaac cgacgccatg ctgcggcggg
    23821 cggcgggctt ggtgaacggg cggccgctgc gcctcgtcaa ggtgtcgcgc cgccgcaaac
    23881 acctgctgtt cgacgtgccc gtggctggtc ccgccaccat cgccgtcatg cgcgagaccg
    23941 gtaccacggc gttatccgtc gacgccggcc gcacgctgct gctcgaccgc gacgagatga
    24001 tcggcgccgc cgatgccgct ggtatcacct tgattggttg cccgcccgaa gagtagcgcg
    24061 gggggcgttc tccgtgtcgc cgtccgatct cccgccatga tactgtcggg gtgatgttca
    24121 ttccggaggt acggtgaata agattcgagc tgccgtcgtc ggcgccgggc agtttgggcg
    24181 gaatcacatg cgcgtgattc acggctcccc acgcgcggaa ctggtcgcgg tcgtcgacat
    24241 cgatccggag cgcgcggccg aggcggcagc gccttacggg tgtcgggcgc ttacagatct
    24301 tgccgctctg aacgggagcg ttgatgcggc catcgtggcc acgccgacta gcgctcatgt
    24361 cgacgtcgcc cttgctctga tgaacgccgg cattgacgtt ctcgtcgaaa aacccatcgc
    24421 gcgggatctt acctcggcac gctacctggc tgaggctgcg gagagcagcc gccggatctt
    24481 acaggtgggc cacctggaac gtttcaaccc ggccgtctcg gcgctctccg cgaacgtacg
    24541 tcttccgtta ttctttgagg tgcatcgcct cagccagttc acgccgcgca gtctcgacat
    24601 tgacgtcgtg ctggacctaa tgatccacga catcgacatc gtgctgtcga tggtcggctg
    24661 catgcctgag gaaattcgcg cagcgggcat ctcgatcctc acgccaaagg tcgacatcgc
    24721 gaacgtgcgg ctctcattcc ccggggggtg cgtcgccaac cttactgcaa gccgtgtgtc
    24781 gacagagcgt gtccgcaaac ttcggctctt tcaaccgagc gagtatctgt cgatcgacta
    24841 tggacgtcag gaagcctttt cactgtcggt gacgccggat cgccggatcg cgccgagaac
    24901 gctcacggtc caaaaaaacg agccgttgca gctcgaactc gagcacttct tcgaaagtgt
    24961 cgaaacccgc agaccgccgc gagtcggcgg ccgtgatgcc gtgcgcgcgc tcgaggtcgc
    25021 gttgggcatt cttggtaaaa ttgaagagca ctcccgggtg gtggcccaat ccctcgcatc
    25081 cgcgcagaaa ggttagatgt aggccttgga cccgcctctc caggcatatg acgaggaact
    25141 gaggctcttc cccgactgga agagccaaga ggtcagcgtg cgctcgttgc gccccttcgc
    25201 ggcctcgctt gtcctgcacg ccatagccat cagcgggctg cttttgttgc ccgccggctc
    25261 tgttctagac cgctccggtc cgcggaccct cgcccagttg cccgaaccgt tcaagctcgt
    25321 ggagccgccg ctcgagctga cccagacgga accgaaccgg gggaaggcca gcaagagcgt
    25381 cgacctggaa gcacttctac cccggccgcc ccttcagctt cctcctgcgc ttccgtcgac
    25441 gaccaggccc gctgcgcgag tccttgcgcc gccatcccct cccactccag aggtcgcggc
    25501 gcttccggag ccaccaaaga tcgacacagc cgggagcagg ctcgcacagc cctccgtaca
    25561 gcctcccggc agcttgaccg cgtctccccc gcagatccag acagaagaga agccgaagct
    25621 cgcttttgag aggccgggtt ctcctgccgg cgagccgggt tcggaaggtc gccagttggc
    25681 gcggctcgcc ccgcccggcg gttccgtctc cgaggtcgga cgagagctcg cgggcggacg
    25741 ctcggctggg gggttggtcg tgggagatct gggagaaggc atcggcgggc tgggcgaagg
    25801 tctgaacctt ccgccgtcgc ccggccggtc cggcagttcg ctcgaactgc tgagcgaccc
    25861 gatgggagtc gacttccgcc cctacctgat caaggtcctc tccgcggtgc gccggaactg
    25921 gtttgcggtg atgccggaga gcgccaaact agggcgccgc gggagggtgg tgatccaatt
    25981 cgccgtcagc cgggacggaa gcgtgcccaa actagtgatc gctaccccct ccggtaccga
    26041 cgcgctggac cgggcggctg tggctggcgt cagcgcgtcg aacccgttcc cgccgctgcc
    26101 tgtagagttt cgcggtggga gtgttcgtct tcagttcgtc tttctttaca acctcaagtc
    26161 aaactgagcg ccgcgagcgc ggcccggagt gttcccgaag tgtccccggt ttttcacagc
    26221 ctcctgtctt ctcttcgtgg gagaatcgag gttccacaat gaagggccgt ctgcttgccg
    26281 cgacactctg ctacgtaatc cttggcgccg tcgccacgtt gaccctcggc gggaagcctc
    26341 tcgcctttgt tctgctcttt ctcgccgcgc ttgccgtcaa aacctggatc cattccgtcc
    26401 ggagcgagta gcatccgttc caaattgaaa cacggcgttc caaaccgaaa cctggagtgg
    26461 ccggagcgcg ttcaaaacag gactccctag tccatgctac tcatcgcctg atcgtcccct
    26521 ttttcggcta actcgttgta tcaccgtcag gtgttcaccg tagtccctcg tctgtgatgt
    26581 tggccctgga tgtgcttttt cttctccacg tcgggccgtt caacccgacg tcgcgattcc
    26641 gggagtaccg gggatcgaat ccgccgaaaa gaaaaaagaa gggtcatcga catgaacgct
    26701 acacgtgtgt ccggacgggg cagtgagaat ccgaattcga agcgtgaccg aatcatcctc
    26761 gatcaccttc cgcttgtcaa agccatcgcc ctacgggtcc atgagaacct gccggtacac
    26821 gtggacctcg atgatctggt gcacgccggc atcctgggtc tgttcgatgc cgccaagaga
    26881 tacaacccga gcaagcgggt gaattttccg agttacgcga agcaccgcat caagggcgcg
    26941 attcttgaca gccttcgcca actcgattgg gcctcccgtg acatgcggcg gcggcataag
    27001 cagatcgagg cggccacgcg cgacctggct gccgtcctga agcgcaatcc gacgcaggcg
    27061 gaactggcgg cgaagatggg cgtcgggatg gatcgctggc aacagatgat ggttgagatg
    27121 cggaacgtcg ggctggtttc cgcgtcgggc cgtggtcccg aaaacgaaga cggcccccca
    27181 cccgattttc cggccgaaga ggagactcaa cccgactcga tctgcgcacg cgagcaactt
    27241 cgcgggcatc tacgggtcgc catggggacg ctgccggaaa ggtaccggaa agtcgtacta
    27301 ctttactaca cgaatgagat gaccatgcgg gagatcggcg aaatcatggg gatcaacgaa
    27361 agccgggtat cccaaatcca caagtccgcc ctgcaaaaaa tggctgaggc gcttcagtcc
    27421 gcggggatca cttcaagcgg agcgttctaa ggatcgcccg gcctacttga tctcgaccga
    27481 gtatggcatg agccgcaaca tgccgccctc gatccaggcg ttgatgtgca cgcctttcgt
    27541 gagtgagcgg aaccggcttt cgagcgccga cactttggac ttgctcagca ggcgctcgac
    27601 gatgctgcgt ttgggtggat acacgacgag attcacccgc tgcgtgtcgg ggacctgcgc
    27661 tttccgccgc gccagttcga tggcttggtc aataccgccc aattcgtcca caaggccgcg
    27721 caccttggcc tgtgacccca gccacacgcg accctgtgcc agcggctcga tctcgtcgaa
    27781 cttccgcttg cgcgcatctg ccaccttctg gacgaaagtc ctgtagttgt cgtcgatccc
    27841 atcccggagc ttcttgcgcg cggcgtcgct gagcggttgg taatcggagt cgagatcggc
    27901 gaaccgcccg cgggtgagca tttccttttg gatgccgagt ttgtcataga ggccccgcag
    27961 gttcaccttg ccgaaaacca cgccgattga tccggtgatc gttcccggat acgccaccac
    28021 cggatcgccg gtcatggcca tgtagtagcc gcccgaagcg gcggcgtcag acatcgacac
    28081 gaccaccggt ttcgcccggc tgagggtatt catctcgcgc cagatttcgt cggatgcaaa
    28141 cgagcttccc cccggcgagt cgatgcgcac caccacggct cgaatggctt catccttcgc
    28201 cacgtcccgc aagagccgga taaaacgctg cgcatagatg ccactttcgc cggacaggtc
    28261 gtccccgctg ccgccttcga tcgagccctc cgcaaccacc agggcgatgc gccgtcccgc
    28321 gggccgttcc gcagccacgg cacggagata atcccggcaa cccaactttt tcagatcgtc
    28381 ctgcgcgagg cgctttctta gttcgctgta tacctggtcc tcgtaaacca gtgcatcgac
    28441 caagcccttc ttcagcgcct cttcgggaag aaaggggccg tcatcgatgg cggccctgat
    28501 ctcgttcgga tctttccgcc tgccttcagc caccgtcgaa atcaggtgcg cgaacagttc
    28561 atcgaggatc gagttcagca cctcccgtgt ctccgggctc atcgacgtcc gtgtgaacat
    28621 gtcgccgaag tccttatagc ggccggcgtg ctcgatctcc acctgaacgc cgatcttgtc
    28681 caaggtcttg cggagataca tcacctctgc acggagaccc tttacgtcga gccagtcttc
    28741 cggggccatg tacacgcgat ctgcggcgct cgcgagataa tactcccgcg tgcgcgccga
    28801 gcgaaggtac gccaccaacg gttttcccga cttgcggaaa cgggcgaggc agccgcggat
    28861 ttcctggagt ttcccccacc cgacgttcag cccgccgggc agcaagacga cggctttgat
    28921 gcgcgggtcg gtctcagccc ggcgaaggat tcgccatacg tcgctgaccg tgagcggttc
    28981 tttctcttcg aaaaaaggaa gcgggatgct catcggcgcc tgctcgggga tgtccccttc
    29041 caggttcagc acgagtgtcg atccgtccgc aatcgccgga ggccgttctg ccagccgcac
    29101 agccgcgaaa agcaggatga tcagcgcaag tcccgcgagg atgaccccgg tcaggacgcc
    29161 gatgaggaac ttcaccatgg tcttttgtcc aatcttaggc gcccgtcagg atcaggtcga
    29221 cccggcggct cacatctttc aggtagggca ggtcgccgcc ttcgagttcc accgtcgccg
    29281 agcctttgta gccgacttcg gccagcgctt tgtagacttc cttccagtcg atgtcgccct
    29341 cgcgcagatt cacgaagtcc gccttcatgt tcttgaagcg gaagtccttg aagtgcagct
    29401 tgcagatgcg cttgttgagc gtgcggatcc agtcctgcgg atagccgtag aacaccatgt
    29461 tgccgacatc gacgtaggcc cgaacccaag ggctcttgaa ctcgtcgacg tagcgcgcca
    29521 tttcaagcgg gctcagcagg aacttgttcc agacttcctc gatgccgatg accaccttca
    29581 ttttctccgc catcgggatc agcttgcgga tctgaacttg cgagcgggtc cacgcgtctt
    29641 tgtaactggt ctgcgggttc acgaccgccg gaaccagcag cacggtatcc gcgccccaga
    29701 gttgcgcgtt cttgaggctc gtctccatgc ccttcatact ctcggcgact accgccgggt
    29761 cggccgagga gagcgggtat ttccaatggg ccgaattcat caccgagtga atccgcacct
    29821 tcgttgcatc cgaagctttc ttgaactcct ccgccttgcg cggatcggtt tcggtgttgg
    29881 gctccatctc ttcgaatccg gcatcgcggg ccatcttgaa acggtccatg atgctgagct
    29941 tctccggcag catgccgaac cagatcgctt tcttgatcgg caagcgtgcc ggagcggcgg
    30001 aagccatcgc ggccgccgct gtcgaaccta gaaacgtacg ccgagtcatt cttatttctc
    30061 ccttttcaat tctgaagtca gaactctact ccgaaatccc tgcttttatg caaactctac
    30121 cccaacgtct gcgccgacct gccggatata ccgcttccct tcttcgtact cttccgcggt
    30181 cttcaggtgt tccagcatca agggggcctc ggaaggcagc ttcgcaagct ctcgaaggta
    30241 ggagcggtaa tcgacctcgc ctctgcccgg cacaacttcg acgaagtgca cgttcatctc
    30301 gaccacccac gccagatcct tcgcgtggca ggagacgatc catggaccaa gcttccggaa
    30361 gcactcctca acgaatttcc cgctctgata gaagcgttcc ggactgttga ttccgttgca
    30421 gacgtccatg tggaccgcga acgctttccg gtcgatcgcc ttgatcaggc gaaggtatgc
    30481 atcgggccca tccggcaagc tccagcccat catctcgagg gtgaatttcg tccgcgtcgg
    30541 cttgacttcg tcgatgaggc ggcggcaatt ttcgaccgtt gcatcaaaga attccttgga
    30601 gaaattgccg ggatgcggac cgtaccacac tttgggattc atcgaaccgg cgatgtccac
    30661 gcagcatcgt gcgcccacgg cctcggcgag cgccaatcgc ccggtaacga atcgcatatt
    30721 ctgttgccgc ttctggggat ccggatcgag catgttgacc caggcgccga cttcggcaat
    30781 agtcaccctt tcggcggcaa aggccttctc gatttcacgg actctcgccg tatcgtcagg
    30841 tttggcgttc gggcagtatg ccgcgctgta accgagcctc cggtgttcgc gggccatttc
    30901 ctgtggatcg tcggtcttca ggaagatcgg gccgccaagc cgaatagggc gccctgaggc
    30961 atggaaagga acggctgcgc cggccattac ggccagtgca ctgcggcggg tcggtctgta
    31021 actcaccccc cgattctaca tcgaagtcaa gcactccctc ggcacgttgt tccgcgcggc
    31081 cgtctccagc ctgtgctgcg cgcaactcag atcaaattgg ggattttgat gatcttcttc
    31141 tgtatcgcgt attggacgag ctgggctttg ttgtggatgt cgagcttacg catcaagtta
    31201 aacttgtgcg cctccacggt tttgacgctc aggttcagtt ggcaagcgac ctccttgacg
    31261 gaattgccct cggcgagcat cttgaggact tctttctcac gcggcgtcag cgttgcgaaa
    31321 cgtggaagcc ggtcggtcga cttgatccgc gtccggaaat catccaccaa ttgtgagagc
    31381 attcgcgggc tgaggtagct acctccccgc gagacgtccc gtatagcggc caccagttgc
    31441 ggcgccgggc tgtccttgag cacgtagccg ctcgctccga cttccatacc ctcgacgagg
    31501 tagtcctcat cgtcatacat ggtgagaaac aagaccttcg tctcagggcg gtttctacgg
    31561 atctggcggg tcgtctcgaa gctgctgggt cccggcatgc cgatatccat caatacgacg
    31621 tcgggccgaa actgaccagc ccgttcgagc gcttcggtgc cgttggctgc ctcgccgaca
    31681 atctcgatgt ctgactcagc ggccagcagc gttttcacac cctggcggaa cagcgtatga
    31741 tcgtccgcca gcagaattcg aatcttggcc atacactcac gctcgattcc ttccgggaca
    31801 atccgggatt ttcgggccgt tcctgtcaat ttctggtgtc actggcagtt cgatgagcac
    31861 ctgcgtcccc ttccttggcc ggctttgtat ttgaaattcg cccctgaaca acgccactcg
    31921 ttctcgcata ccagggaggc catgggattc gcttttccca gacttttctt cgacccggaa
    31981 gccgacgccg tcgtcggcca cccggagcct caatcttcca tcggcatgca ccgcggaaat
    32041 gtttacacag ccggccgaag agtggcgtgc gatgttgttg gcgcactcct gtatcaggcg
    32101 atataccacc gcggcgacac gctccggcag ctccgtcagg ttggaggtcc tgacgcggac
    32161 ctccatgccg tgaaagcgtc tcagacgtgt cgcgatctgt cggagtgcgg ccggcagacc
    32221 aagctgttcc agaaccgccg ggcttaacgc cccgatgagg cgccggatct cgctgatcgt
    32281 tcgctccgtc agtgcccggg tctccgccaa tccggtcttc aggtactcca ggctctcggg
    32341 aatcagccgt tcgagcaact ccagttgcag gcggatgcag agcagtgact ggcccgcctc
    32401 gtcgtgcaac tccgcgctga ttcgcctcct ctcccgctcc tcggtcgcga ccaccgtcgc
    32461 gcccagggcc cgcacctgtt cctcccgcgc cgccaggtct tcgacgagac gggccttttc
    32521 cgctgccagc agacagcgct ccccagccgc cgccagcagg tcctgctccc ttgggagcca
    32581 ctcgtatggc cctgcgaagc cgaactggat cacccccacg gcgcagtcgc ctgccgccag
    32641 aggcaccgac cagcacgagc ggtaccgccc ccgccatttc gggtcgaggg cgagcccctc
    32701 cacccgtccg caacggcacg ccggtacgga gagcttgccg gaccggaact gggtccccgt
    32761 cacccgcaac ggccgcgctt ccagcttgtc cgtgccctcc acggccgcgc gcagaatcca
    32821 gtcacccgag tcccaatccc ggaggaagag cgctcccgcc cgtgcccggc agaacctggt
    32881 aagcgtctcg atcatccttg gaaggagctc atccatcgtc ttcgattcca gttctgcccg
    32941 gaacagctcc tggtacgcgc tggtttcagc ctcccgaacc tggtagaacg cgttgttcag
    33001 agtcagcacc acgcagaact gcaattgctc acgggcccag ccgagattgg cgcgttcctg
    33061 cgcaggcagg ggtcgaaaca gcggcgcgag gaggcggtcg tactcgccaa gcgcggtgat
    33121 gatctggtcc ggcgccagat tcagctttgc cagccggcgc ccgttgtact ctacctgctc
    33181 gaagaagtcc acgactggtt ttccagccgc cagcagtcta gacgccgcac ctggggtaat
    33241 cgcggcgaga gcccggcgct gcaccgcctc atatccggcg cgccgcaaat gcattaggaa
    33301 cttgcgctcg agcgaggcgg cccggccctt caggatctgg gcgaggcgtt ccagatgcgc
    33361 ccgcaactgt tcgccgagca cgtcggcggc agcggccaac gcaggcggat tctcagagta
    33421 cgtcaccgcg tcatccatcg gacgcgcgac gtctattctt tagcgccctt ctccttgaga
    33481 aacgccacga tcccagcgta accgttttcc atggctgctg tcgtgggggt tttcccgccg
    33541 cgggttcggg cattcacgtc agctccgttt tcgaccagaa gacgcacgac ctccaaccgg
    33601 ccccacgatg ccgcatggtg gagcggtgtc gctcccgagt cgctgtcctg ggcgttcacg
    33661 tccgcacccc ggcccagcag cagcgccacg acctcccggt gtcctccaag ggcagcgtcg
    33721 tgcaggggag tgcctccggc gctgttcctg gcgtttacgc cggctccatg cgcgatcagc
    33781 agtgcggcaa tctccgcatg tccgttgagt gcggcgtcgt gcagcagggt tgagccgtta
    33841 gtggtgttgc ccgtgacgct gattcctcgt tccaccagca tccggacgag gtcggtttga
    33901 ccgcccagca cggcctgttg cagccgctct accgcgcctc gtcccagcgg ctgcttgcct
    33961 gcccggtcga gcagaacttc tgccacacgg ttgtttctgt accggagcgc ctcgtcgagc
    34021 ggtgtggccc ccgagccgtc tttggcttcc gggtcggcgc ctgcttcgag caggatcgcc
    34081 acgacttcgg catgaccctt gactgccgcc tcgtgcatcg gcgtcgtgcc cgactcccgg
    34141 ttcttcacat gcacgtccgc gccgcgcgcc accaggagcc tcgcgatctc tgcctgccct
    34201 ttccatgccg cttcatcgag aggtgtcgaa ccggagttgt ccttttcgtt aacgtcggcg
    34261 ccccggttga gcagaagctc cacgagatcc cggtagcccc ggtttgccgc caaatggagg
    34321 gctgtctgcc cggagctgta acgtgccctg atatcggcgc cgttgtcgag caggagcgcc
    34381 gcagcctcgc gatggttcgt gacaactgcg tagtgcaggg gagtcgaacc ggcctcggcg
    34441 tgacgggcgt tcacattggc gccgcgctct accagcagcc ggatcacatc gagatggccg
    34501 ccccaggcgg cgtcgtgtaa cgcggtgccc ccaagcgaat cccgggcatt gatgtcggcg
    34561 ccgccgtcga gcaatgcgcg caccttcgcc gcgtctcccg cacgtgccgc gtcgtgcaac
    34621 tcgcctgctt ccacaaacgg cacgattccg gccgccacca cgagcacggc aagccgcata
    34681 gcttcagact atcaccggcc ccgagctgct caggcgaact cccagcgcac ggccggcacg
    34741 ccttctcctt tcgagttcgc gtacaggcgg atccagtcga actcgattgt cccgccggca
    34801 tcgccgaggt cgaaccgcag cagggccaaa tagccacccg gcttgaagga cacaagatgt
    34861 tcgtgccact gcccgtcggc ggcgaagtca acgctcgccc ggttcttcgg attgctggcc
    34921 atgtcctcga ttgtgctcca cgcaaacgca cggaccggta cgtccttcga gcgggccagg
    34981 aaccggacgg ccatctcccc atcggcggcg acccagcttc gcgccacttc gtttcccttt
    35041 ccggaacatt gaacccgcag cgcaccagcg gtcttcgaaa gcacgcactg tcctttgccg
    35101 caccagccgt ccgcctgagc gcggtacttg tactcctccg gtagtgcctt ctccggcgac
    35161 gcaccctggt ctccggtctc acggatccac cggtcgagcc ggttcttcat gtccgtcagt
    35221 gtcttctctt gctccggcag ggccgccagg ttgtttatct cgtgcggatc ggcttccaga
    35281 tcgtagagtt cctgcggcgg ccggcgcgcc gcgaggaaga gggccggcgg cccggttagc
    35341 ttgccatcct tatcgagctg ccgcatcgcc tgaagcgtcg ggtatgacgt gtctttgtag
    35401 acgttcggtt gtgcataggg gcgctccggg aagaagtttc gaatgtactt gtacttcttt
    35461 gtccgcacgc agcggatccg gtcctcggtc tcgtcgcacc ggtcgcgcgc cgcgacgatg
    35521 aagtctcggc ttttttctcc ggatcccagg aagacctgcc cctgcatgtg gatcgggggt
    35581 tcgataccgg caagccgaag cgaggtcgcc ggaatgtcga tcgcactcac catgtcgtcc
    35641 cggacgctcc ccggctggta tgcttcgggg cggaaccgtt ccggaacccg gacgatcagt
    35701 ggcacatgaa tcccgccgtc gtacaggaac tgtttgtccc gcggcatggg cctgccgttg
    35761 tcgccgaaga agatgacaat ggtgctctcc tcaatccctt cttccttgag gcgctcgagc
    35821 actttcccga ctttgacgtc cagatgctgc gccgaatcga ggtagagcgc ccaatccaac
    35881 cggacggcgg ggtcatccgg gtagtacggc ggaagttcga ccttagcggg atcggtcggc
    35941 cgctccgtga agcggtgaaa cgcacggtgc gtctcgctga agttgatctg tgcgtaaaag
    36001 ggctgccctt cgccgcgctg gttccagtcg gtcccgtcaa agaccttgtc cacctggaaa
    36061 ttgaagtcgg ttttgccggt gcccttgacg ccagccgcgg cggtgaggac gttcgatgtg
    36121 tgataaccgg ccttccggaa gtagtggggg aacagatcga ttccgtccat cagccggtat
    36181 ccgtcgcttc ggtggctgcg gtggtgatga gcgccgatgc tggtctggta catgcccgtc
    36241 gccaacgctg aacgcgatgc ggaacaaacc ggcgccgtga cgaacgcatt cgtgaaccgc
    36301 actccttcgg ttgcgaaccg gtctatgttc ggcgtgtgcg ccagtccgtt gccgtagcac
    36361 cccaagtctg tgctcagatc ctcggcgatc agccagagaa tgttcggccg ggtctgtttc
    36421 ggaccgcgcc tgcaacccgt ggcggcgagc gccagcccgg cggcgccacc gaggatctgc
    36481 cgccggctcg ggaagttgtt ggaattcacg tgcgtgttca tcgaactcat agctttgggt
    36541 tggccggcac taccggcttg ggcttcccgc tgcggtgcgg cgtcagccac acctcgagcg
    36601 ccagtttggt gaccttctcc ggaagccgca ctaccagctt ccgcgttcct tcgttctttg
    36661 cctgtggcgg gggctgagcc gtggagacga catcgaacgt cgcctcagcc ggcgaccgaa
    36721 tttccgccgc caatgtccag ccacccttcc gcagctccgc tttcgcgccg tcgaggctga
    36781 cctcggcgtc tgtcaccatt ccccacaggg cttgaaccgg ttcggtggct tccagcgagt
    36841 cgaggacgag gacatgcttc cgttgcacca gtgccagcgt cctgtccatc cgtttcactt
    36901 tgccaggata cgcctgagag aggtcgattc ggacccacgc ggaattgggg cggaaccggt
    36961 gcccgataat cctcgcctcc gcgcgggtgt cctggttttc atcatcgatg aggagcgtgt
    37021 tgtgagattc cgttcgaagc cggtagtagt tccagcgctg tttcccaaag aatccgggca
    37081 gattgtagtc gtccggcccg agatcgatcg cccaccggac accacccgcg tccagcacaa
    37141 aggccccgag gtccagatgg ccgtgattcg ccttgttgtc gccgcctttt acgcccacga
    37201 aaatcccgtc ggtgctctcc caattgcttc ggaggaacgc gacctgcacc ccctggaaga
    37261 gcgcgtgcgg cggccaaccg gcccgttttg ggctctcggc ctctgttcgg taccacacca
    37321 ggtccagtgc gtcgccgccg ctcgttttca gaagttggtg ttgctgccac gcatacgctg
    37381 gctgtgcgaa ccgctgcgcg agccagaaca tttccgccgc cggtcccacg cggtcgctcg
    37441 catcggcgta attgaaactg cggtgtgacg ggcctgtgga gtagatccgg aaatgccccg
    37501 cccgatcgaa gccttgcgac gccgagaggc cgaaatcgat gcccagcgcc gttttcagcg
    37561 ctgccagaaa gtagacggcg taccgggtgg cgtagtgcca gtatccggga ccttccgccc
    37621 aaccgccgtc cggggcgtag gatgccatcg ccgccggaag cgacttcact gcgttccgca
    37681 acaccttgct cgcgcgctgc ggttcatcct cggcaatggc cagcgcaccg atcccgatgc
    37741 cgccgttgca cacctggttc cagttgtact tcgaggttac ccaccagcct tgtttggcgt
    37801 agatcgggat cgcctggtcg agccctttgt cgacgatggc ccgccggatc catgcccgtt
    37861 cctccggcgt cagcccgtgg tagagccagt cgtaggcgat cgcgaaagcg tgcgtcatct
    37921 ccgccgtgtc gaggaagtga ctgggattcc agtccgggaa atacgccgcc gcacgaagtt
    37981 ccttcacagc ccgatcgagg tagcgccgct cgccgtccaa tctgtatagc agcgcaaggg
    38041 tatagatccg gtcgaggcac cgccggctct gcgccagcaa gcgggggcca actactttgt
    38101 actcaacggg cggctcgccc tccagtttcc gggcccgctc gacgagtgat gcgtacaccc
    38161 tccgagcgcc ctcgtcggcc gcgatgagtg tgcgtatccg atcgatgtcg ctgtcgagcg
    38221 cgatgatccg cggatgtccc ttccgcaacg tctccagcgg ttcgagagac tgcgccggca
    38281 gaatcgccgc tgacgcgagg aagattgcca tcaagcggag catgaactcc ttatatcacc
    38341 gctggagcgg ctcgcggcgg gacctgtgta gaatagttct cagctaggag gacccagttc
    38401 catggagcgc cgcgattttc tgaagtccgc cgttgccgga acgattgccg cccgcgaggc
    38461 tgccgcgaaa aagggagaga tgccgaagcg gaagtatcgc gataacgtga aactttccgt
    38521 gatcgggttc ggcggcatcg ttgtgtgcgg aatggagcag aaccttgccg accgcaccgt
    38581 ggccgaatcc tgggaccgcg gcatcaacta ctatgatgtg gccccaactt acttcgacgg
    38641 tgaggccgag accaagctcg gcatcgcact gaagccgtat cgaaagaagg ctttcctggc
    38701 ctgcaagacg acgattcggg acgcgcaggg cgccgcaaag gaactggagc agtcgctgaa
    38761 gcggcttcac acggaccact tcgacctcta tcagttccac gctgtcagct cgatggagga
    38821 cgttgagaag atcctcgccc ccgcgggcgc cgccgagctt ttcacccggg cgcgcaagga
    38881 tggtaaggtg cgctacctcg gcgcctcggc gcacaacgcg gaggcggcca ttgcgctgat
    38941 ggagcgcttc ccgctcgatt cgattctgtt ccccgtgaac ttcgtctgct tccagcaggg
    39001 aagcttcggc ccgcaaatcc tcgccaaggc gaaggagaaa ggtgtggcgc gccttgcgct
    39061 gaaggcgctc gcgcaccatc cctggccgaa agggacggat cggaagaagt ccggatacga
    39121 gaaatgctgg tacaagccgg ttgacgatcc cgaactcgcc cgcaaggcgc ttcgctttac
    39181 tctgtcggag gacattaccg ccgccgttcc tcccggtgac gagcgcatct atgccatggc
    39241 actcgacatc gccgccgctt tcacacccat gaccgccgcc gagcgccagg aactgctcgc
    39301 ctcatcccag ggtacggcgc ctatttttaa ggtgtaggga ccttctcaga gtgctggaaa
    39361 agttcgctgg ggcgccccct ctcttacggt cgcggttcct gaagcgctcc gaagcactcg
    39421 atcacgtctg cggtgcatga ccggcgagga agcgtcccgc cccagcgatt acactgaaga
    39481 agttggacgg aattgggacg tcatgagagt tctcgcgtct tttgcgtgtg ttgttgcgct
    39541 cctagcgatg ggcgcttgcc gccgatcggg aaaaggcgga ggaaccactg cggcgcggag
    39601 gatcaacgtc gtcctggtca ccattgatac ccttcgcgcg gaccggctgg gatgctacgg
    39661 ctatgcacaa gtggccacgc caaacttgga caaactcgca caaagcggca cgctttttga
    39721 aaacgccgtc acccacacgc cgctcaccgc cccttcccac gccagcatct ttaccggaac
    39781 gtatcccacc gtccacaaag tgcgggacac cggtggattc gtactccagg gtccccatgc
    39841 gaccctggcc gaaatcctcc agcgaaatgg ctgggcgaca gcggcattcg tgggcgcttc
    39901 cgtgctgaag aaggggtttg ggttcagccg ggggttcacg gtgtacgacg accagatgcc
    39961 caagttggag cccggccggc cggccggcga ctacgccgag cgccgggcgg gcgaggtggt
    40021 cgacagggcg gtggagtggc tccaaagaca ggacgataag ccgtatttcc tttgggtgca
    40081 cgtcttcgat ccgcactctc cgtacgatcc cccttcgccc ttccgtgagc agtaccgggg
    40141 cagggcgtac gacggggagg tggcctattc cgatcgggag ttggggcgcc tgtttgacgc
    40201 gattgccagg aaatccgggg gtgagaatac cctggtcgtg gtgctttcgg atcacggaga
    40261 gagcctgtcc gagcacggcg agtacagcca cggcgtattc ctttacgatg cgacactgcg
    40321 aatcgccttt gtcatgtccg gaccgggcat ccccaagggc atgcgggtga agcagcaggc
    40381 cagagccatt gacgtactgc caacgatcct ggaactcatc ggcggcaatc tgcctgcggg
    40441 cgtgcagggt ggcagcctgg ttcctgccat ggccggcaag acggaccccg cggcctggtc
    40501 ctatgcggag tccctttatc ccaagatcaa tatgggatgg gccgagttga gagggatccg
    40561 cacgaaccgc tggaaataca tacgggcgcc caagccggag ctttacgacc tctcgaggga
    40621 catcgcggag accgcaaacg tcgtttctca gcatcccgag gaagtgcggg atctggaagc
    40681 ccggctgcgc gacgtggcag cagaagggga gaaagtggag accaccatgg tggatcgccg
    40741 caccatggaa cagttgaagt ccctgggata cgtcggcggc tcatcgcgga cccagtacac
    40801 cttgaagggc gaaggggcgg atccgaagga ccggacgggc atcctcaagt tgctctatct
    40861 cgcgatttcc ccagactggg gcggtaagat gtctcagcgc atccccacgc ttcgccgggc
    40921 gctggcggaa gaccctggta acccgaccat ctactatcac ctcggggacg agtacgacaa
    40981 ggccgggcgc tccgccgaag ctatgaaact ctaccaggaa ggaatccgta acggtgtccg
    41041 gaatgcttgg ctgtactcgc gcctgggcaa cctttacatc cggcagggca accacgccga
    41101 ggcgatcacc gccttcgaga gagcggcgaa actcaaccct tccgactgtg aaagcctgag
    41161 cgatctggga atggcctacc tggataccgg ccggccgacc gacgcggagc gcgtctataa
    41221 atggtgcctc gcgaccgagg aagaatacgc tccggcctac aacggcctgg ggcttgtcgc
    41281 ggtgcagagg caggacatga cggccgctcg cggttacttc gagaaggccg tggagtacga
    41341 tccggacctg ctggaagggc aactaaacct cggccgtatc tataaaatga tcggggccaa
    41401 cgcacgggcg cgcgcctgtt ttgaggtgtt tctggccaag gccacaccgg cggaatacgg
    41461 gcacatcatc cccaaaatca aagcggagct ggcgcagcta cagtagtctt ccgcccggca
    41521 acagccgctg ttcatctgtg gagaaatgca acaactctcc acagcgagcc tccacatttt
    41581 tcgccagaaa ccaaaataag ggcttgacaa gaggataata gcacctcaaa atagccaacg
    41641 aacttgcttt aggtgtgctt tggtgcaagg accttaatcc tgatcagtcc atatcagtcc
    41701 ataaaggagg aatccctatg agggtgtcta gtacaaagct gaacatattg ttctgtcttt
    41761 ttctggcagt cctgttagct gcgccggcgt tgccgcaggc cagtacgtcg gccgtcaacg
    41821 gaacggtgcg cgaccagtcc gcggcggtca tcccggcagc cgctgtgacg ctcacgaata
    41881 ctgctacaaa cgtgacttcg agaaccaccg ccaatcaggt tgggttcttt ctgttcacgg
    41941 gtatcaaccc ggggccgtac cggataaccg tcgaggcgcc tggattccaa aagtttgagg
    42001 ggactctcac cgtgcaggtc cagcaggccg cggtggtgaa catcaccctg aacgtcggcc
    42061 aaacggcgac tgaggtctcc gtccaggacg tcaccccgat ggtgaccacc gacagcccca
    42121 ccctcgggca cacgctggag cggacgcgca tcgagcagtt gcccatcaac ggacgcgctc
    42181 tcacgtcgct actgcaaacc gtgccgggca tggagggcac acgcgcattc ggcttgcgcc
    42241 aaatgtcgtt cgagatggtt ctggacggtg cggctcttgc cgaccgttac tcctggaaca
    42301 ccgtgacccc ccggcagccc ggattggact cggtccagga gttccgggtt gaaaacaatg
    42361 cctcctcggc caagtttgcc cgccccacct cgatcatcgt gaccacgaag ggcggcacca
    42421 acgagattca cgggacggcg ttcgagacca atcggaacag cggctatggc gtggcgcggc
    42481 agcggcagga tacctgggag aaggcttctt ttctgaaccg caacgaattc ggattcagcg
    42541 tcggagggcc ggtttacctc cccaagattt acaacgggaa gaacaagacc ttctggttca
    42601 cttccacgga gtggtacagg gaaatttcga gcatgaccct ccaagcctcg ctcccgacgc
    42661 aggcgatgcg ggacgggaac ctggccggat tgacaaacga tgccggcaac gccattacca
    42721 tctacgatcc ctggaccacc gattccacca cctgggctcg ccagccgttc ccgaacaacc
    42781 agttgccaca gagcaaacag agtcccctct acaagtacct gatcgagcat acgcagttgc
    42841 cgaccaggcc ggaggtgaac ggctactcgg ccagcaacta cgtcggctcc tggtctccct
    42901 tcaactacca atggaccacc tccagccgca tcgaccaccg attcagcgac aaggactcgt
    42961 tctatggccg ctacacgcag ggcaactaca acaggatcgg ccagttttac ggcataccgt
    43021 ccaagaactg ggacgtagtg cccggcaaca cccaaacgaa cctaagcccg aacaagagct
    43081 tcgctttctc tgaagtccac accttctctc ccacgttctt caacgagctg ctggtgaccg
    43141 gcacgcgcac gagattcgac gtcgccacgg gcgatcccac ccgaatgtac gactcggagt
    43201 tggggctccc caatcctttt aacgtgaagc agtggccagg cctgtacaac ctgggattcg
    43261 acggctccta tctgtttgag acgcagaacg gcaacggctt ctacgccttt tacggcattc
    43321 tggacgacaa tgcgacgaag atcgtaggta ggcacgaatt gcagttcggc ttccatttcc
    43381 gttccgacca gatgaacttg ctgccacagc agcaacaggg cgccggcagc ttcagttggg
    43441 cctcgaccgg cactgctctc tatgatccga gcacctcgcg caccaatccg ggcacgaccc
    43501 cgttcaccgg ggatcagttc gcgaatttct acctgggaat tgggcggtat aacaaccagt
    43561 tgaaccgggg gatgttctat gcccggtcga aggaatacgc cgggtctttc caggacaact
    43621 ggcgcgtgac gcccaggctg acgctgaacc tgggcttgcg atacgacctc ttcccgccgt
    43681 attacgagaa gaacaatgcg gtgtccagtt tcgacccgtc cgacaagtcc gtggttctgg
    43741 gcgcgcccct cgagaagttg tatcaattgg gatacacgtt cccggccatc gtggatcgtc
    43801 tgtcccagat gggaatgaag ttcaagacgt accaggaagc gggactgccc gagcacatga
    43861 tcaacacgtc caaagacggc tgggggccga ggattggttt tgcgtaccgc atgggtgatg
    43921 gagccaagtc cctcgtgctt cgcggcggct accgcatctc gtacttccac ttcgtcatgg
    43981 gcggctgggc ggcgcggatg cgcatgaatg cgccgatgac cgcgcggttt tatggtccca
    44041 acggggatct ggaacagtca gcctattcgc cggacggcat cggcaactac ctgctccgct
    44101 cggtgcccgt ctacatctcg ggccagaaca cgaccaacga cctggtgcag cccaccaacg
    44161 cgagagggtt ggcccgcggc tgctgtggtg tctcgtactt caacacagac atgcccgacc
    44221 cgcgcgtgca ggactggaac ttcacgatcg agaaggagat catggagaac acggtggtgc
    44281 gcgccggcta cttcggaaac cacagctccc ggctggagca gctctaccag ttcaacaatg
    44341 ccacgcccga ttacgtctgg tacatgacaa ccggcaagcg gctccccacg ggcgagtact
    44401 cccaaaccgc caggaggttc ttcgatcaga ccacgtacgg aaccctcgaa cgctggcaga
    44461 ataccggctg ggggaacggg aacggcatcc agctcgaagc ggagcgccgc tacgcgaaag
    44521 gatacgcgtt ccagttgttc tatgtgttga ctaactcgat gatggccggt ggcggtggct
    44581 attcgggcac ttcgccgatt ccggaagtga accagttcat gcccggagcc gttccgacgg
    44641 acatcgacgc gcgcaacgcg ttcctgaact accagcgaga cacaagtgtt ccgaagcacc
    44701 gcgtccgctg gaatttcctg gtggacctgc cgttcggtaa gggcaagccg ttcctgggag
    44761 acgcgggaag ctggctgaac cgcctggtgg gcggctggca ggtcgccggc ataggctcgt
    44821 tacagagcac gtacctgacg ctgcctaccg gcttggtcac gaacggcaac aagatcgaag
    44881 tctacggtta caagtacccg atcgaagact gcaccagcgg gaaatgctat ccggggtacc
    44941 tgtggtggaa cgggtacatt ccggcgtaca ggatcaacag caccgatccg aagaccggca
    45001 aaccgaacgg ctacatgggc gttccggcgg attaccagcc ggcggttcaa ccgctttatc
    45061 catatccggc tgactacttg acccggtccg gcaccaccga tcccatgtac ccctggtacg
    45121 gcggcaacac gacctggatc actctgaacg atgggacacg acagcgtact acctggggag
    45181 aactcccgat gttgcgcaac cagtacctgc cgagcgtcct tcagtggggt ctggacgcct
    45241 caatcttcaa ggctgtcccg atcacggagc gcttcaacgt gcgcatcaac gcggatttct
    45301 tcaacgtgct caaccatccc ggcaatccca acagctttct tgggagcacc ggcatcctga
    45361 acacgcggaa ctccggcagc ggcgcacgcc aaatccagtt gacgctgcgc ttggcttggt
    45421 agcagcagct ttttgacacg ggccggaatc gcgtatgcgg ttccggccct ttttcgctga
    45481 aaacggtggt gggcggtgag ggtctcgaac ccccgacctt ctgggtgtaa accagacgct
    45541 ctgaccaact gagctaaccg cccgctccga ccacgatagc acccacgccc gtacggcatc
    45601 aattcgctac aatcgtcccc tgtgacgtcc gcaatccttc ccgagcacat tcgtgccgcc
    45661 gccgagcgga tccgccccat cgcccatcgg acgcccgttc tgacgtcgcg cagcttcgac
    45721 ggcgaagcca gtattcgcgc gttcctcaag tgcgagaatt ttcagcgcgg cggtgcgttc
    45781 aagatccgtg gcgcctcgaa cttcctcttc tcgctcgcgc ctgaacagct ccggcgcggc
    45841 gtcgtggcct actcgtccgg caatcacgcc caggcagttg cgattgccgc ccgcgccacc
    45901 ggggcctcgg ccacgctggt gatgccggcc gacgctccgc ggtcgaaaat ggaggccacc
    45961 cgagcgcagg gcgccacgat cgtcacctac gaccggcatc gcgagagccg cgaggagatc
    46021 ggcagccgga tcgccgccga aaccggcgcg acgctcgtgc cgcctttcga tcatccgtgg
    46081 atcatcgccg ggcagggcac caccgcgctc gaactactgg aggacgcgcc ggaactcgac
    46141 gcgctgatca ccccggtggg cggcggcggg ttgctctccg gatccgccat cgccgcgaag
    46201 tccgtgcgtc ccgcgatccg catcttcggt gccgagccgg agagggcaaa cgacacctgg
    46261 cagtcgctct ccgccggccg ccgcgtcgag attcccacgc cggataccat cgcggacggt
    46321 ctccgcgcga cccgtccggg cgagatcacg ttcccgatcc tccagcgtca cgttgaggag
    46381 attctgctcg tcagcgaaga cgagattaag gcgactgtcc ggttcctgct ctcgagactg
    46441 aagatcctcg tcgagccgag cggcgccgtc gccgccgcgg cggtcctcta tcggaagctg
    46501 ccgtcgaata tagagagcgt cggaatcatc ctctccggcg gcaacgtgga tttcgaaacg
    46561 cttcaggcgt tgtaagagac gttccagccg ccgtcccacg agacgttctc agggattgcg
    46621 tccgccgtcc cgatgaaggc gcagtattcg acgcgtgctt tgctgaggga gggaatccag
    46681 cggtagcagc gctcgccgaa cagcgtgttg cgctcaatca tcagccggcg cggctccggt
    46741 atcggcgaaa ccccgaactc catgccgcgc gtgagagtct tgccgttcca cggaggctgt
    46801 tcgcgcgagt agttctcctc ccagatgccc agccacggga agtccgcacg ggaccagacg
    46861 tagccgaaga gcactttcgt ggatggcgac caggcagcga agtaggcctg cgttcgcgct
    46921 ggatccatca agtgcgtggt gaagccgccg gaaaccgcgg cgctcgtgta cacccgcagg
    46981 tccgaggttc ccccgttgtt gcgcggcacg tgcggccagt tgaactcggc gcccgtcttc
    47041 atatagccct tgccgccggt agagtcctcc tcgtccactt tcgaacgcgt cgcggacgca
    47101 cggaactcgg tcaagccctt ctcgagaaac gggggaccaa gggttgcgtg ctcggtccag
    47161 gcgatggggc gatcgaagac tgagagattc tccacggtct cgacgatgcg gactacgcca
    47221 ctacctggcg acagccggat cttacgctcg atacgcaact gagccagcgg caattccgcg
    47281 cggcaattca tctccgacga atccgcggag atctcgtagg gaaccaccga ggcctctccg
    47341 tggacggtca gcccagcagc cgcctcctcc ggcgacggtc cgccgaagat atccatgcag
    47401 aggttgtggc cgatgatccc ggccagcagt ttgctctcgg cgtcatcgcc gtactcggga
    47461 tgcttcacgc ggttgtaggt ggatggctcg atggacggcc agggcggcgt ccacagcggg
    47521 ttcacctcgg tggctttgtg caggacttcg gcaatgtgcc caccttccgc cgtgacggtc
    47581 acccgaaggc tcgctgtctc gatctggaag gcgcggcggt tgcggtactc ggtctcggcc
    47641 atggcgtccg taggatactc caaatgcggc gccggccgcg atccctactt cgagcttgct
    47701 tagctcttgt ttgcgggtac cgctccgggg gtgcggccca tctgctccat ctcgttgaag
    47761 taaacccgtc gcccctcgtc catcgcgaga ttcgccagca tcaccgcagt tgaatcctgg
    47821 agaccgatgt taagatccgc cttcgggcgc gcgccggttt tgcagctttc gaagaagctt
    47881 tccatttgca cgtcgaccgg gttgcgcgat tcttccggaa ccatgacgcc cttgctgtac
    47941 agccagcggc gggcaaactt cagctccttc tcgaggaacg attccttgcc gctgaactga
    48001 tcccgctcca gcaagatcgg aaatcccttt gcgccgcgac cggtgctggc gagggtggcg
    48061 cctgcgacca cctcttcctt cttcgccgtc ttggccggag tgacctgggg cggcttgggt
    48121 tcgatgtacc acatgccgat agacggctcg tcgtcggtgc cgacagtgat ctcgatcgtc
    48181 ccgccggtgc ccatgatccg ctcgccaaac tccgtgcggg tgctaccgaa cagaggaagt
    48241 tgcttgttgg tgctgatcga cgtgtagatc aacttctggc cccgcgggta cctgaaaatc
    48301 aactgaatgt tgtcatagac gtcgcggccg tccttgatga attcgagccc gccgacgccg
    48361 acgacgaact cgggatgggc cccaaacatc cagtcggcca cgtcgatctg atgcgacgcc
    48421 agctcggctg tcagaccgcc ggaatattcg cgaaacagcc gccagttgcg ctccctttgg
    48481 cggaccgggt cgggcttcat ctgccagccg ggattccggt gccactgagc catcacgtgg
    48541 gtgacgttgc ccagcatgcc cttgtcgacc atctgtttgg cggtctgata aaacttgctg
    48601 taccgccgct ggagaccgac ctggagaatc tgctttgggc gctcattcac aagagtgcgc
    48661 agcgcgtgca cttcttcagg tttgaagacc aggctcttct cgcagaaaac gtgtttgccc
    48721 gcaagcagtg cgtcgcgggt gactgggaag tgcacgtaaa gcgggactgc aataacaact
    48781 gcgtcgatgt ccttccgcgc cagcaactcg cggtaatctt tataggcggt cgggttcgtg
    48841 cctgccgtct gaacgccgtt cttcaggttc ggctcaaaca catcacagac cgagacgcac
    48901 cggccgttgt cgaggccggc catgtgtttt aacagatatg tcccgcggcc accggttccg
    48961 atgacgccat acagtacctg gtcgccggcg gctttcacct tctggatgag tggcccttga
    49021 aatgaggagg ccgctgcggc ggccgtcact gcccccgccg tcttcacaaa tgttcggcgc
    49081 gtggggtcct tcttggcagg cataaagaca gtcctctcaa tattagcgat gcctaactcc
    49141 tgattcgagt ttcttcaagt ctatccggtt cagaaagccc ttgcaattga gcgtctacag
    49201 cgcggcttca cccgcatcgg gactttcccg atgactgcgc gcgcaccact ccgcgcccgc
    49261 cagaacgatc atcgcagcga gaaaactcca caagacgatc gtgaccgagt acacgaatgg
    49321 tccgtattcc ctggccagct tcgccctcca cagcggccac gtcaggatat tcaggtattt
    49381 tagcccttcg agcagcaagc cgaccagaac cgcggcgggc aggacgtcgg ccacggggat
    49441 cttgcggttc ggcaacagcc agtagatcag gaacagcatc agcatggaca tcggcacggc
    49501 cgcggccttg aagaacaaca ggccgaacgt cgtaacgatt ttcgggctcg cgcccaacac
    49561 gctcggccag attgcgtagt tgagagcggt cagtgtcgtg gatgcgagcg ccagcgtgcc
    49621 gcaggcgaag atcagccccg tgctgatgac ttggttgcgc aggaaactgc gatgctttgc
    49681 cactccccag gcgcggttga gagcgacttc gagcggctcg aaaatcccgt tcgccacaaa
    49741 caggaggagg atgaccgaaa caatctggaa cgggccccgc tgctccactg tcgcgtggag
    49801 atttcgatgg acaaactctc cgaggttccc ggggaagtag tcgctgaggg cgaggttgac
    49861 cgcagtgacc gccgccggcc acttcagcac gtgcttgcac agcgaaagca tcacaatgag
    49921 gaaggggaaa aacgagagca ggacgctggc cgccaccgag aacgcataca cgtgtacctc
    49981 ggtttccatc cagtatcgaa aagtgggacg aaggcgccgg aggagttccg gcatcggctc
    50041 ttgcctgagg agttccaact ttcagcttac gccagcccga cggccagaaa ccaccaaaag
    50101 aaaaccacaa aagacacgaa ggcacaacgg gttcttttca atttctgcac tttgtgtctc
    50161 tttgcctttg tggttatgat acagtccaca ggagacaaat ttatgttcag gcctgtcgcg
    50221 cccgtgtgtt ctgcaattct tgtggtggcg gcgttcgttt cccagccggc gctggctcaa
    50281 gagcccaccg tcgacgcgat cctcgaacgc tatgtccagg ccctaggcgg caaggcgaat
    50341 ctggaaaaac tgaccagccg cctggccacg gggatttttg aagtccctga gtgggagacg
    50401 aagggcccca tcgaggtcta ttcgaaagcc ccgaacaaaa cgatgaacac cacggacttc
    50461 gccggctatg gcgtcgtggc tcggggattc aacgggacgt ccggatggag taaggacccc
    50521 gacgaaggtc ttcgggacgt cagtggcgga gaactcgctg aaatgaagcg agcagccgag
    50581 ttttaccgcg aattgaagct gcgcgagcag tacgccaaga tgaagcttgc cggggcccaa
    50641 aaattggccg ggcaggacgt gcaggtgatc gaggcgacca cgcccgaagg actcctgcac
    50701 aagctttact tcgacaccca aagcggcttg ctcgtccgcc acgatatgca gagtgagatc
    50761 ccgggcggaa agatgactac ggtcgtgatg ttgggagact accgggacgt ggacggtgta
    50821 aaggtaccct ttaccatcga gcagaacaat ccgaacttcg gtttcatcct gcgcctgaaa
    50881 gaagtc
//
LOCUS       DATIFQ010000433        30370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_911290, whole genome shotgun sequence.
ACCESSION   DATIFQ010000433 DATIFQ010000000
VERSION     DATIFQ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 30370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30370
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911290"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VLE22_21655"
     CDS             <1..934
                     /locus_tag="VLE22_21655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17071.1"
                     /translation="DTIDPAGFIENAKRYREQFWTELMGRFDDPLLAPNPRTRKIFDN
                     ARWKGYEVVLDVWPDVFAWGVLLVPNGIRLGERRPVVVCQHGRGGVPRDVIEGNDTAY
                     GNFAAELADRGYVVFAPHNLYRGEDRYRWLCRKANTVKGTLFTFIIHQHDQILRWLET
                     LPFVDGQRMAFYGLSYGGETAMRVPAVLEKYSLSICSGDFNQWTWKVAATDERFSFMY
                     TIEWEMPYFNLGSTFDYAEMAYLIFPRPFMVERGHHDLVGRDRWVAYEYAKVRWLYAQ
                     FNLPERTSIEFFNGGHAINAEGTFRFLDKHLRGR"
     gene            1010..2500
                     /locus_tag="VLE22_21660"
     CDS             1010..2500
                     /locus_tag="VLE22_21660"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17072.1"
                     /translation="MVDPMKVTLIGASGWYAFDLYRRVFADERMRPIELRIWNRNPET
                     GGPIAEMLDYVRRESGIRFDFNLYENRKEALRGADYVLFASCVDYPRASVVDREVCAR
                     FGVHVLEGETMSPAGLMNTFRHGPIAVDVASDLMEVSPNAVIIPVSNPLYRMCDLWHR
                     YANVRFIGHCDGIIHTKTDLAKAMGIDPADVEVIAGGLNHLTFILKMWNRRTGEDLLP
                     RLDDAMPRIRQLGPFGFRFSNVVYRLLGHYISPGDNHVADQLPFVSRRMQETTPIPSL
                     DVIFPPKDVVHSGKAANINAIANVSQHIRSPKVLEAFLHPARFEESGDWMLALHGRTP
                     GYHMEAINVPNDGHISNLPKGAIVEVPGRIDRTGPRGFTIGDLPLAIATLCHRMVAVH
                     QLAVDAFVHRSREAALQSIALEPTVKDLYVIEDLFDALLEANGRYLDADFVADLRAKS
                     RHRRVALVEPAPDNLLRPDAPAPEVSPTLDIVAGTFWGAEMGNLAD"
     gene            2647..2994
                     /locus_tag="VLE22_21665"
     CDS             2647..2994
                     /locus_tag="VLE22_21665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSB17073.1"
                     /translation="MGRAAAITRTEIPPGTLYMLILRTLAHLGPMHGYGVAQYIQQIS
                     QDVLQVEEGSLYPALQRMLIKGWVTAEWGQSDNNRRARYYRLTPAGKKQMNAEISEFE
                     KVMRAITRVIQPA"
     gene            3010..5589
                     /locus_tag="VLE22_21670"
     CDS             3010..5589
                     /locus_tag="VLE22_21670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17074.1"
                     /translation="MEWLDELWRRLLFPFRRRQFDRDLEEEMQFHLDMKAREIGPVAA
                     RRKFGNSTLLQEDSREVWGWTVAEAWLDDLRYAARVLRKNPGFTAVVVLTLALGIGAS
                     TAVFSVVHAVLLRPLPFREPNRLAMVWETWEKRGDDRVVVSYDNFRDWKEQSQSFEYM
                     AVFVGDSARMTVGDEPVEIQASRVSGGFFPALGIQPMLGRTFVPEEERMSAPLVTVLS
                     YGLWQRLGGDPNLAGKTVRFDREVFTVVGIMPRGFAFPLDSELWFPFASDDNRNTRGT
                     HYLRVIGRLKPGVTAVQAQSEMQTIAGRLKQEHPKENGGIGANVVPLMDQIVGEVRHA
                     LLALMGAVACVLLIACANVANLLLVRATGRRREIALRLALGASRSRVVRCLLTESVVL
                     SLAGGALGVAAAYWLVRAFVAFDPIQLPRVHDVVVDGWVLLYALAAAITTGVVFGIVP
                     ALRASKPDLGSGLKEGPGVRGAGEFRKSRGRNALAAAQIALALVLVVGASLLLRSFVM
                     RVSVPLGFQPEGVLAVELPWSAHRRVDELLERFRALPGVLAAGAATAFPQNSAGTSCD
                     ACVDIEDEPKREGKQYDTGLMVATPDFLRAAGMRLRQGRFFARGDGAEAPKVVVINEA
                     LVRRDFPGQDPIGRHIRWSGGAWSTIIGVAGNVKGFGVAGDPMPSVYFPHQQADWGNG
                     VQVLIRTSVPPLRLVGSVRAEIRSWNRRIVIGKLDTLDNMLESSVAVPRFYLMLVAGF
                     AVLAMMVSAVGVYGTINYSVARRTHEIGIRMALGAEPGDVLVMVLGQGLAITAAGVVI
                     GLAGAWMSTRVLETLLFGIRPSDGVAFACGSGVLSVTVLLASLIPARRATRVDPLKAL
                     RCE"
     gene            complement(5627..6022)
                     /locus_tag="VLE22_21675"
     CDS             complement(5627..6022)
                     /locus_tag="VLE22_21675"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HSB17075.1"
                     /translation="MDELLRRGIRLFNDREFFQCHEVLEEAWTPERGPRRIFLQGLIH
                     LAVGFYHCQRLNPAGAIRQLRKGLRKLAAYLPSCEGIDTMRLHREAHETLEQIEGGER
                     LPLYPQIHTVTSSRAGRKSSRSPLSSNDR"
     gene            complement(6043..6942)
                     /locus_tag="VLE22_21680"
     CDS             complement(6043..6942)
                     /locus_tag="VLE22_21680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HSB17076.1"
                     /translation="MATTESVPKQFKITDYKGKADPDWCPGCGDFGVLASLKAALAEL
                     NIPTHQVVTISGIGCSSNLPGYIDTYGMHTLHGRALAVATGVKMANHELHVIITGGDG
                     DGFGIGGNHFMHTMRRNVDLTYIAMDNQIYGLTTGQVSPTSRLGMKTKSTPFGSVERP
                     VNPILLALSAGATYVARGFSGEPKHLTELIKKGLQHTGFSFIDVFSPCVTYNHDNTYP
                     WFRDRVKKLELDSSYDATDLNAAMQRAQLWGDEIPIGVFYEDSSMPALHQLEPILDEG
                     GPLAHRDVRVPADIARQFVEELM"
     gene            complement(6953..8728)
                     /locus_tag="VLE22_21685"
     CDS             complement(6953..8728)
                     /locus_tag="VLE22_21685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HSB17077.1"
                     /translation="MKDTFAVAIGGAAGQGVATPGNIFAKILARRGLHLLAYNAYQSI
                     IRGGHTFLTIRAGVEKVTSFGDRIDLLIPLNQDTMDRHLRLLGPGAAVIYNGDNVKPG
                     DAAEGVRLCPLPVGELSGNSRAKVEQNTLAVGAALSMMGVGFQLLEEILTQQFKKKGD
                     AVVAENVGVARAAYQYATQHFGPFERPLPMTERRYAVLTGNMALAMGGAAAGVKFYCA
                     YPMSPSTGVLHWMSAHARKAGIMVRQVEDEIGVVNMAIGAAHAGVRAMCATSGGGFAL
                     MSEGLGMSAMIETPVVVIDVQRAGPSTGVPTKTEQGDLWQVLGAAFGDYPRVIAAPTD
                     ILDCFKLIPEMHNLADRFQCPAIVLSDLLLSEGHTSVDPADLDFEVPIDRGELITAPN
                     GANGSYKRYLITESGISPRAIPGVPGHTHTVATDEHDEDGVLISDEFTNTVKRRAMME
                     KRQRKVAGIEAAVPPPVLNGPRDADVTLIGWGSTKGVIQEAIEILAEEGITANHLQIR
                     WLVPLHGEAIVDILRGSRRTIIVENNYSGQFARYLRSETSFVADGHIRKYDGEPYMPH
                     HIVEAVKEQLARGTKLSVPMHEINV"
     gene            complement(8881..9336)
                     /locus_tag="VLE22_21690"
     CDS             complement(8881..9336)
                     /locus_tag="VLE22_21690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17078.1"
                     /translation="MKTLRFLSMLALGVTAWAADPAIGTWMLNTAKSKYSPGPPPMSA
                     TITYEETADGIKRTGESVDAEGKKTSFEYTAKYDGKEYPVSGSDEFDTITLHRVNDRT
                     TQATLKKSGNVVSSARRVVSKDGKEMTLTITGTNSKGQKMRNVAVYEKQ"
     gene            complement(9497..9649)
                     /locus_tag="VLE22_21695"
     CDS             complement(9497..9649)
                     /locus_tag="VLE22_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17079.1"
                     /translation="MSGLMMALLLALIGTYGLMSHAVTEGTLEFEIRSALGAARLDPA
                     TALCSE"
     gene            9761..10471
                     /locus_tag="VLE22_21700"
     CDS             9761..10471
                     /locus_tag="VLE22_21700"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HSB17080.1"
                     /translation="MTGQVFCGTSGFAFASWKPDFYPEKLPAKKFLSHYASRLNAVEI
                     NYTFRRLPAATTLENWVAETPASFRFVLKGHQRITHFGRLVVSDFSHLFFKAIDSLRV
                     QKRLGPILFQLPPNLAADPDLLKRFLDAAPDDLRLAFEFRNKSWFADPVYRILSDRRA
                     ALCLAESEKLTTPPVITSDFVYFRLRKPDYTPGERREIAAKAAELRSDGKDVFLFFKH
                     EDTPAGALYAEELLRSNA"
     gene            10728..11162
                     /locus_tag="VLE22_21705"
     CDS             10728..11162
                     /locus_tag="VLE22_21705"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSB17081.1"
                     /translation="MPKEGARFATLRETYDQVFHHLAQQVRELQSLMSQPATDERAVE
                     EAKRRVEQAQATYRETRDLLAWSLLFGDVKSASATDLEALARAAAIGIGPLGRAGGLD
                     HRSQVEQLAHQLWEQTGRPSGKAEEHWYLAERLAERLIHRAA"
     gene            11310..12443
                     /locus_tag="VLE22_21710"
     CDS             11310..12443
                     /locus_tag="VLE22_21710"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HSB17082.1"
                     /translation="MKITPEKSMIVETQRVRFDSVSLDCGAALAPVDVAYETYGQLNA
                     AKSNAILIVHALTGDAHAAGVGHDAGKPGWWDSMVGPGKAFDTDKYFVICSNVLGGCQ
                     GTTGPASINPVTGCPYAMSFPVITIGDMVRVQKMLVDHLGIERLLSVSGGSMGGMQAL
                     QWTVAYPDSVVSAIPIASTARHSAQQIAFNEVGRQAIMSDPDWNQGDYYGKKPPARGL
                     AVARMVGHITYMSDESMREKFGRRLRNKDAYGFDFEIDFEVESYLRYRGSRFVDRFDA
                     NSYLFITKAMDYFDLANGTVSLGPVMETTSARFLVISFTSDWLYPSYQSQEIVNALRS
                     RNRDVAYCELPSNYGHDAFLVDVAEQTEIIRGFLASTHKEKAG"
     gene            12440..13069
                     /gene="metW"
                     /locus_tag="VLE22_21715"
     CDS             12440..13069
                     /gene="metW"
                     /locus_tag="VLE22_21715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HSB17083.1"
                     /translation="MTTLVQAKRKPFVREVLGRSDFAIISELVEPDTKVLDLGCGDGE
                     LLHWLAENKSVDARGIEINSSLVQRAIARGVSVYQGDIDQGLADYPDGAFDYVILSQT
                     LQETRRPLKVLREMLRVGRRLIVAFPNFGHWRVRLAHLVSGRAPKTRLFPHHWYESPN
                     IHFLTVLDFEELAKQEGWTVERRIFVTGHYVGRLVPNLMAEVAVFLIRK"
     gene            complement(13093..14487)
                     /gene="thiC"
                     /locus_tag="VLE22_21720"
     CDS             complement(13093..14487)
                     /gene="thiC"
                     /locus_tag="VLE22_21720"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HSB17084.1"
                     /translation="MRSEWVARRVKDSVRTQMRYACQGVVTEEMEHVTRLEKIPADVV
                     RSEVARGRMIIPANIRHTNLEPMAIGVASKCKINANIGNSAVTSNIEEELEKLRWSVK
                     YGADTVMDLSTGGDIPAIRKAIIGNSPVPIGTVPIYEALTRVRRVEDLTPGLLLEVIE
                     EQAEQGVDYMTIHAGVLVQYIPLTTRRVTGIVSRGGSILAEWMVKNHRQNPLYECFED
                     ICKIFQKYDVSFSLGDGLRPGSVADASDEAQFAELKTLGELTKKAWEYDVQVMIEGPG
                     HIPMDQIQLQVEKEMEMCYEAPFYTLGPLVTDIAPGYDHITSAIGAAMIGWYGASMLC
                     YVTPKEHLGLPNRDDVKAGIIAYKIAAHAADVARHRPGVRERDDELSRARYRFDWNRQ
                     FELSLDPETARAMHDETLPDEGFKDAHFCSMCGPKFCSMNISAKIETFTDADAKSVLN
                     SPSNLVELSPSSAD"
     gene            complement(14665..16767)
                     /locus_tag="VLE22_21725"
     CDS             complement(14665..16767)
                     /locus_tag="VLE22_21725"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSB17085.1"
                     /translation="MSGLTRRRFVQSAAAAAPAAAGAAGSAAGGLASGARVSLNGTWR
                     FRLDPGKGGESAGWHLPTAGGEGWSEVTVPHTWQVAQESHAHLGVAWYRREFDVPAQW
                     AGSHVRVEFEAVYHSASVWLNGKPVGSHLRKGYTAFELDLTAGLKYGERNVLAVRADN
                     SFDPAMLPRVDSYDWTPDGGITRPVNLLVTSPVFLQRIEVDAMPEPGEASAWIRVRAV
                     IRNAGGLPAGVSIGWEAAEEASGRVVLRQPNAASVAVRPGATETISLPEAVLAEPRLW
                     HFDQPNLYRLSARIEAAGKPVHELSDTFGIRRIEVKEGGLFLNGERVRLMGVERMAGS
                     QPQFGMAEPAWWIEHDHNDMKELNCVFTRVHWQQDRRVLDYCDRHGILIQTEVPSWGT
                     KTFEGMGDTPSHEILENGLEQLREMIGRDRNHPSIFAWGLCNEVNGQNPPAQKFIRAM
                     AAEARKLDPQRLLTYASNSLQSNIGRDVAGELDLISWNEYYESWMQGDVPAMRRNLEE
                     IHRVFPGKMVVISEYGLCECRPEHTGGDEKRIRVMREHTNVVRDYDWVGGAIFFCYND
                     YRTHIGDKGEGPLKQRVHGVVDLYGERKPSFEALRSESSPVAALTLSGTASSMTAVVT
                     ARGTLPAYTLRGYRLRWIVYGFGDLPMEQGEAPLPELAPGKSATLHFSAQEKAPRRIR
                     VDVLRPTGFSALTALWKG"
     gene            16864..20253
                     /locus_tag="VLE22_21730"
     CDS             16864..20253
                     /locus_tag="VLE22_21730"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HSB17086.1"
                     /translation="MLSKNWDHRKPLYDFIVVGSGYGGAITAARIAAANLNPKPLVCI
                     LERGREWPVGSFPDTIEGIAGEIRSDINPLGLHEFLTYRDISVWKGNGLGGTSLVNAN
                     VAIVPDAEVFDSLGWPKSLNRAELMPYYQRVRETLDANPHPNALGLPKVQALKRRADQ
                     IGREVLALDIAVNFHPDGPNKHGVPQKKCTNCGDCITGCNVGAKNTLYMNYLPMAADA
                     GAEIFTQCKVEWVEKLTGGGWRIHGRRYRSKSRTEKFTFDAANVILSAGSINTTEILL
                     RSEMHALSVSPRLGSGFSGNGDFFAIAYNGDYRTQVLGFGNHLNHLRASSPPGPTIVG
                     AVRYNTGVPVDQRITVEDLSFPKGLVEGAARLFLTQPGVDTDTGDETQERARALQDSD
                     PFAEIQADGALDHTMMYLVMGIDDARGSMVFEKTLFEPDGRMRIEWDDAGRQVVFTRI
                     NEELRRHSRALGATFIENPTWSFLNLRHLVTAHPIGGCPIGEDHIQGAVDEFGRVFAG
                     DGSVHSGLFVADGSLLPSALGVNPFLTISALSERIAERKVREMQGEAYPAPKVSVSLS
                     GIDPVEAAGWKEAELERLFGRAESQGIDVMLNSGKREIDAARRTVHNDEYWKGFFPRG
                     HILNAMSAVIFTGFKKRFFKQGKKYAGVTSDTDGRINARNSLEELNLKKQTGDLKPGR
                     YILLRYVDPPWQGYYDVFKVINENLLIGRVYFGEYPNGIRMFTFPMTRSYGFSAMTVE
                     DHRQLFASGTVPAKEELNGAWRMDVISNANQAGGLAWLSFDLKPDGRLESRYHLMGLM
                     EGLVTPTFVADHFRLDDFTDFHDEIRKIDNDLFIGKYVTELPAAVAGLLPASSLGLLH
                     VEGDDRKRFGMYYLLTRAEKTDVPTTTLLRPLLETHLPDGLGMVFDEEMEGWYSPGVF
                     TSGPGRKGDLEIAARIPDSGKPAGGSDCRFDLRMTVRDLNEFIDGAEHEARASGSISF
                     EQFEGRGPVTFPVDDRRSYFNYLRINPATGEAEMRYHLEFRSDDGRAFTFEGRKYMQK
                     DDSGGPRAMREVLDDYTTLYCHVYEDGTQGRRELGTGYLKFRTFEDIFAVGNLAGFLR
                     SFQVTGTDDPLLQLRGQARFLAFTGQFVQQEYDPVISSVPD"
     gene            complement(20236..23049)
                     /locus_tag="VLE22_21735"
     CDS             complement(20236..23049)
                     /locus_tag="VLE22_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17087.1"
                     /translation="MRNRAWLPLLCLICASPAACGPVEFGRIEVERAIAERGLKPSQV
                     RFTAEITPDAPETYRIEPGRITGGDLRGLMYGLLEAAAQIRERGRLFAVKGAPATPMR
                     GIRWFLHNADLERDWYYSHEYWRDFFAMLARNRFNRFNLVFAHQTEYLAPPYPYWVEL
                     PEFRNVRAPGLSSAERDRNLEMLRFISHTAAEHGVDFTLGIWEHNIQPGMEPSVEGLT
                     AENIGPYSYAALKKVLAACPAIRSVQMRTNSESGIPSDKQVEFYRDWVFRAVAEAGRR
                     VTLDMRGWAMQPGMLDAAVTSGAPLRLSSKYWAEDLGRPYQPAETWPGYSYLDFLKKP
                     RPYEFYWEVWGLGSNRLLLWGDPDFVRRMTPTFRLSDSIGFEIDPPMAQKGFGNRPGK
                     WGVFAAGRENRIFWKHEFERYWLFYLLWGRLSYDPKTPDSAWMAELKRRFGTAAADVL
                     EAYRGASGVLPEIVAAHLADPNMYIWPEINPGGLIDAYKDVRPSDWRMIAGIPEAVRN
                     RIEGMVSAKQTALDTAQRLDELAARTEQAVARARSRLRGEHREWSGSEPDFSVLSSLA
                     RYHARKQTAAYRLEWFYQTGDPAALADAKRELSAAAEVWEGLARLTDGLYPEEMAFGP
                     DDVGHWKHKLAYVRHDLKIVEEREKVLERFGQFEFGFDFGGPVTRPHGSSYRNDPYVW
                     MNTVEPRFLPVDPGTAYSEERGYGWATEGEREAVAARLATYHEVRAVVTKPGPLPHDA
                     LTGDSIRGSGRQVFRVRTASNGERQVLFLNAGGEVSEVSGTATNGVLDIAFPEGEWNV
                     SGLVVKVPGAAPKFPVLTAPKPMPRPVFTHIPPHMFVPGRPLSVALGRTASASVKSVR
                     LHYRPLNQLAKFKTLEAAPGRAVFTIPPEDLTPDFDLLYYFEVLDKQGGGWFHPDPRT
                     ATPYYVVTAAKPGSSGVHSVGDR"
     gene            23122..23286
                     /locus_tag="VLE22_21740"
     CDS             23122..23286
                     /locus_tag="VLE22_21740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HSB17088.1"
                     /translation="MPVYIFLCRDCKKEFTKILRISELEQGGIRCPHCNSEKVEQKVA
                     AFSAVTSKKS"
     gene            23529..27200
                     /locus_tag="VLE22_21745"
     CDS             23529..27200
                     /locus_tag="VLE22_21745"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HSB17089.1"
                     /translation="MQRRVMMRVLISPVLVLCGLGGAQTVQHSTAQLTVDGKLGDSYW
                     QAVQANTLIPSQSGVFADMGGDVRLGFREGYLCLAARLPEPGGKVLARSIGRNPVWEK
                     DAMESPEVEDRIVYTLRYRSTGGAERNVTIAINPWGAYRVEDRGQVIPAAKILSAAEV
                     TGQGWSVEAAVPLGMLDVERAAATQVGIRAERIRSRRPLAPEFRWSWPAGSEYSNVVL
                     PPRSFAASGQNAPVLQPPPLGNTDPPLEVGRVLQVPPAVAEWDDPAWKGIPEFELARN
                     ETSPRSPRHSTRVRWMHDGRKLALQFRMDEPEPVVARAGGRDSAVTGDDHVAVYLATT
                     GSAFLEIATNSVGAIRDAVGRGPHSMGTRSDWNADFEVQANIRHGHWVARLNIPLEQC
                     AAALGETGVPRNWRILLVRYRAPRPGEAAETSTLPGLEGASSFYGPIRYQALNLLKGD
                     PTEVKLPHAVQKVPPAGLAGELAAMEPYVWPSLDRRYQGVRTMLQRYMQMRTEGAVLA
                     ERRAWEQVETREDWERFRDERIKGLRESVGKFPPDRPSLDVRVSVRQKGDGYVLENLA
                     FQSRPGFYVTANLYLPERVTPQIPAIIIVHSQHYPKTQGELHDMGEMWARTGCAVLII
                     ERPGYGERAETTTWYRQAYGSRFTFTKQLFLIGESYSGWAAWDIIRSVDFLHDRPEVD
                     KNRIILLGSVAGGGEPAAVAAALDPRITAVVPFNYDQGHVRVHGDSPGQIARQFSPWL
                     VAASVAPRKFVRAFEFGWEGAEEPDYPELWVDGLKRSEKVWGFYGARENLASSQAYGL
                     IRLSMERVSHCFSIGPQQREPLYAIFNRWFGIPFPSQKDLEILPDSQLSTNPVREAAR
                     RQEAQRRRPHADLLSIPPDLSAKIQRRKIHQIAHEMGKEMLAAARQRRQSLGAEERVQ
                     RLRDELQPMLGDISPKGSPRSKTVWTRALDGAVVEAVSLEVEDGIRVPLFLMRPQGRP
                     ISPVVVALAQSGKERFLANRSKELEQLLRAGVAVCLPDVRGTGETASSSELDDPDTQP
                     AIREFDLARSLLGSRLKDLRTVLVYLRNRSDIDAKRIAVWGDSFAPANPRNLWLDEIE
                     FEAGPQIQYWADPLGAHLALLVALYENDVRAVAARGGLVGYLTVLDEPYTYVPMDVIV
                     WRLLKSADIADIAGALAPRPVVLAGLVNGRNILTDATDLDRVLEPAKRAYESAGAANR
                     LTLRVESENMAAWLIAATK"
     gene            27311..27907
                     /locus_tag="VLE22_21750"
     CDS             27311..27907
                     /locus_tag="VLE22_21750"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HSB17090.1"
                     /translation="MFSKIVRRSHMYLALFLVPWILMYALSTMAMNHREFFKQFFGEQ
                     PVAWERERELTYPGTFPEDAKPNDVARQILVSLGMDGAHNANRSKDGARLTIVRHDPI
                     TPQRITYTPADGRLVIESQVFRTPAFLERMHRRRGYQSDYRLDDTWAFSVDLVIAAMI
                     FWVASGLWMWWEMKVTRRLGFAFAASGVAIFALFLSTI"
     gene            27921..28517
                     /locus_tag="VLE22_21755"
     CDS             27921..28517
                     /locus_tag="VLE22_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17091.1"
                     /translation="MTFHLLTRRIHLCLGLFLLPWFFLYGVSSIPFSHAPYFQKLYED
                     GVPQWTVRFEKPYEIQVPKEGDMRATGARILADNGLKGSFGTYRPNEKVLETYLFTFW
                     TASRVRYFTEEKRLLVEDRRSRWDQILTGMHARGGFEQDSILSDAWAIFIDVVCIGMV
                     LWIASGIYMWWKIRSTRVWGWLALGGGLAAFAIFLAAL"
     gene            complement(28534..28728)
                     /locus_tag="VLE22_21760"
     CDS             complement(28534..28728)
                     /locus_tag="VLE22_21760"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HSB17092.1"
                     /translation="MESQSSAGPVPVELLLRKLAEGATVQDLLDAYPRLTAEDIWAAL
                     AYAAKTYAALPHFVGIADLG"
     gene            complement(28926..29327)
                     /locus_tag="VLE22_21765"
     CDS             complement(28926..29327)
                     /locus_tag="VLE22_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17093.1"
                     /translation="MLHALQAVIYVAVIVLARMQSAWGFGAGCIIAAFWNYINLFVTT
                     FIKAGVQQFSILLRTGELPRPDLLIAVVAAAGHFLLIGACLAGFLRTRPELRQWGQFV
                     GGGVLAVGYFALIIITTGPQYVGLLKRVFHL"
     gene            complement(29558..>30370)
                     /locus_tag="VLE22_21770"
     CDS             complement(29558..>30370)
                     /locus_tag="VLE22_21770"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB17094.1"
                     /translation="LMAAVKAARPTSAIFAFADSDGRPGRLWLRSLVEVLQDDRAGAA
                     TGYRWHVPERGGLWAILRSVWNAVIAGRFGPGPLRFAWGGAMGIRREVFVRARVADFW
                     KDAVSDDYRLSDAVLKAGLQIRFAPGAIVAETAGTGFREFCEWTRRQMLLTRFHRPAL
                     WWMALISHLVYCGAAAACLIELARGQLAAAVAVVTLWGLGMWKGARRAALARSVLKGY
                     EGWFRRYGWVYTWLPPLTTWVWLGSLLSSAFGRVIHWRERSYRLTQRRATRA"
BASE COUNT         5626 a         9531 c         9341 g         5872 t
ORIGIN      
        1 cgataccatc gatccggcgg ggtttatcga aaacgcgaag cgctaccgcg agcagttctg
       61 gacggagctc atgggaagat tcgacgatcc tctgctggct cccaatccgc gcacgcgcaa
      121 gattttcgac aacgcgcgat ggaaggggta cgaagtcgtg ctggacgtgt ggccggatgt
      181 cttcgcctgg ggcgtgctgc tggttccaaa cggcatccgt ctcggagagc gccgcccggt
      241 ggtggtctgc cagcacgggc gcgggggcgt gccgcgggat gtgatcgaag gaaacgacac
      301 agcctacggt aacttcgcgg ctgaactggc ggatcggggt tacgtcgtct ttgcgcccca
      361 caacctctac cgcggcgagg accgctaccg ctggctctgc cgcaaggcca acaccgtgaa
      421 aggcacgctg ttcacgttca tcattcacca gcacgaccag attctgcggt ggctcgagac
      481 gctgccattt gtcgatggcc agcggatggc gttctatggt ctcagctacg gcggcgagac
      541 ggccatgaga gttccggcgg tgctcgagaa gtactcgctg tcgatctgct cgggagactt
      601 caaccagtgg acctggaagg tcgccgcaac cgacgagcgg ttcagcttca tgtacaccat
      661 cgagtgggag atgccctatt tcaacctcgg cagcacgttc gactacgcgg aaatggcgta
      721 cctgatcttc cctcggccgt tcatggtcga gcgaggccac cacgatctgg tcggacgcga
      781 ccgctgggtg gcgtatgaat acgcgaaagt gcgctggcta tatgcgcaat tcaacctgcc
      841 ggagcgaacc tcgatcgagt tcttcaacgg cggacatgcc atcaacgcgg agggcacgtt
      901 tcgcttcctc gacaagcacc tccgcggtcg ctgaacagtc gccggtggcc ggcgaatgat
      961 gcgatgagcc atgtcagaat atgagtccat ttcagagtgg gaggcgccca tggtggatcc
     1021 aatgaaagtg acactgatcg gggcaagcgg ctggtacgca ttcgacctct atcggcgggt
     1081 cttcgcggac gaacgaatgc ggccgatcga gctgcgcatc tggaaccgaa atcctgagac
     1141 cggcggtccg attgccgaga tgctggatta cgtgcggcgt gagtccggga tccgctttga
     1201 cttcaacctg tacgaaaacc gcaaggaggc gcttcgcggc gccgattacg tgctcttcgc
     1261 ttcgtgtgtg gactatcccc gcgcttcggt ggttgaccgc gaggtctgcg cgcgcttcgg
     1321 agttcacgtg ctcgaaggag aaacgatgtc accggccggc ctgatgaaca cgtttcgcca
     1381 tggccccatc gcggtggacg tcgccagcga cctgatggaa gtgtccccaa acgccgtgat
     1441 catcccggtg tccaatccgc tttatcgtat gtgcgacttg tggcatcgct atgccaacgt
     1501 acgcttcatt ggccactgcg atggaatcat ccacactaag accgacctgg cgaaagcgat
     1561 ggggattgat ccggcggatg ttgaggtgat cgccggtggt ttgaatcacc tcaccttcat
     1621 cctcaagatg tggaacaggc ggaccggtga agacctgctg cctcgtctcg acgatgctat
     1681 gccccggata cgccagcttg gcccgtttgg ttttcgcttc tccaacgtcg tatacagact
     1741 gctcggccac tacatctccc ctggcgataa ccatgtcgct gatcagttgc cgttcgtcag
     1801 ccgccggatg caagagacca ctccgattcc cagcctggac gtaatctttc cgccgaaaga
     1861 cgtcgtccat tccggcaagg ccgccaacat caacgcgatt gcgaatgtgt cccagcacat
     1921 tcgcagtccc aaagtcctcg aagcgttctt acatccggcg cggtttgaag agagtggaga
     1981 ctggatgctt gcactccatg gccgcacacc gggctatcac atggaggcca tcaatgtccc
     2041 caacgacggg cacatctcca atctgcccaa gggtgcgatt gtagaggtcc cggggcggat
     2101 cgatcggacg gggccgcggg gcttcacaat cggcgatctg cccctcgcaa tcgccacgct
     2161 ttgtcatcgc atggttgcgg tacaccaact ggctgtcgat gcgttcgtac accgcagccg
     2221 cgaagcagcg ctccagtcga ttgcgctcga gcccaccgtg aaggatctct atgttataga
     2281 ggacctgttc gacgccctac tcgaagccaa cggccggtac ctggacgccg attttgtggc
     2341 ggatctacga gcgaagagca ggcacaggcg cgtggcgctg gtggaaccgg ccccagacaa
     2401 cctgttgcga ccggatgctc ccgctccgga agtctccccg acgctggaca tcgtcgcggg
     2461 aacgttctgg ggcgctgaaa tggggaatct ggctgactga tggaatcggc gctcgcgagc
     2521 cggcttcatt gaaggtcgga tattgcgtgg tgcggtccgg gaacacttgt cgtaaccaga
     2581 caatacagcc caaaataccc cttgacacag aggctcaggg gggctcaaac taccagtagt
     2641 tttactatgg gacgagcagc tgcaataacc cggacggaga ttccgccagg aacgctctac
     2701 atgctgatcc tcagaaccct tgcccacctg gggccgatgc acggctacgg cgttgctcaa
     2761 tacatccaac agatttcgca agacgtgttg caggtcgagg agggatcgct ctatcccgct
     2821 ttgcagcgga tgctgatcaa gggctgggtc acagccgagt ggggtcagtc ggacaacaat
     2881 cggcgggcgc gctattacag gctgacgccc gcaggcaaga agcagatgaa cgcggaaatt
     2941 tcggaattcg agaaggtgat gcgggcgatt acgcgcgtga ttcagcccgc gtgacggtgt
     3001 gtgagcgaca tggaatggct cgatgagttg tggcgcaggc tgctgttccc gttccggcgg
     3061 cggcagttcg accgcgatct tgaagaagaa atgcagtttc atctggacat gaaggcgcgg
     3121 gagatcggtc ccgtggcggc gcgaaggaaa tttggaaatt cgacgctgtt gcaggaagac
     3181 agccgggagg tctggggctg gacggtggca gaagcatggc tggatgacct caggtacgcg
     3241 gctcgcgtgc tacggaagaa ccccggattt acggcggtcg tggtgctcac gctagcgttg
     3301 ggcatcggag caagtacggc ggtcttctct gtggtgcatg ctgtgctgtt gcggccgctg
     3361 ccgttccgtg agccgaatcg gctggcgatg gtttgggaga cgtgggagaa gcggggcgac
     3421 gaccgcgtcg tggtctctta cgacaacttc cgcgactgga aggaacagag ccaatcgttc
     3481 gaatacatgg cggtgttcgt tggcgactcc gcgcggatga cagtcggcga cgaacccgtg
     3541 gagattcagg cgtcacgtgt gagcggcggc ttcttcccgg cgctcggtat ccaaccaatg
     3601 ctggggcgga ctttcgtgcc cgaggaagag cgtatgagcg caccgctggt gaccgtgctc
     3661 tcgtacggtt tgtggcagcg gctgggaggc gacccgaacc tcgcgggcaa gacggtacgg
     3721 ttcgatcgtg aagtctttac ggttgtcggt atcatgccga gaggcttcgc cttcccactc
     3781 gattcggagc tatggtttcc cttcgcgagc gacgacaacc ggaacacgcg cggtacgcat
     3841 tatctgcggg tgattggaag gttgaaaccc ggcgtcactg cggtgcaggc tcagagcgag
     3901 atgcagacga ttgcgggccg gctgaaacag gagcatccga aagagaacgg gggaatcggc
     3961 gcaaacgtgg tgccgctgat ggaccagatc gtgggtgagg tccggcacgc gctgctagcg
     4021 ctgatgggtg cagtggcgtg cgtgttgctg atcgcgtgcg ccaatgtggc gaatctgctg
     4081 ctggtacgcg ccaccggaag acgtcgtgaa atcgcgctac ggttggcctt gggcgcaagc
     4141 cggtcgcgcg tagtccgctg cctgctcacc gaaagcgttg tcctgtcgct cgcgggaggg
     4201 gcactcggag ttgcggcggc gtactggctg gtgcgtgcgt ttgtggcgtt cgatccgatc
     4261 cagttgccga gggttcatga cgtggtggtg gatggctggg tgttgctgta tgcgttggcg
     4321 gcagcaatca cgacgggagt cgtgttcgga atcgtgccag cgctgcgggc gtcgaagccc
     4381 gacttgggga gcgggctgaa agaaggtcca ggtgtgcgag gggccggcga gttccgaaag
     4441 agccgcgggc ggaatgcgtt ggcggccgcg cagatcgcac tcgcgttggt gctggtggtc
     4501 ggcgcgagcc tgttgctgcg cagctttgtg atgcgggtca gcgtaccgct cggcttccag
     4561 cctgagggag tgctggcagt cgagctcccc tggtcggctc accggcgcgt ggatgaactt
     4621 ctggagcgct tccgtgcatt accgggagtg cttgctgcgg gcgccgctac ggcattcccg
     4681 cagaattccg ccggcacctc gtgcgacgcg tgcgttgata tcgaagacga accgaagcgg
     4741 gagggaaagc agtatgacac cggactgatg gtagcaacac ccgattttct ccgcgcagca
     4801 ggcatgaggc ttcgacaggg gcggttcttt gcgagaggag acggagcgga agcacccaaa
     4861 gtggtggtta tcaatgaggc cctggtgcga cgtgatttcc cggggcagga tccgatcggc
     4921 cggcatatcc gatggagcgg cggcgcctgg agtacgatta tcggggtcgc cggcaacgtt
     4981 aaaggttttg gtgtggctgg agatccgatg ccgtcggtct actttccgca tcagcaggcg
     5041 gattggggca acggagtgca ggtcctgata cgaaccagcg tgccgccgtt aagattggtg
     5101 gggtctgtgc gcgcggagat tcggtcgtgg aaccggcgaa ttgttatcgg caagttggac
     5161 acgctcgaca acatgctgga gtcgtcagtt gcggttccgc gattttacct gatgctggtg
     5221 gcggggttcg cggtgctggc gatgatggtg tccgcggttg gggtgtacgg caccatcaat
     5281 tactccgtgg cacggcggac gcacgagatc ggcatccgga tggcgttggg cgcggaaccc
     5341 ggcgacgtgc tggtgatggt cctcgggcaa gggctggcga tcactgcggc gggcgtggta
     5401 attggactag ctggcgcctg gatgtcgacc cgcgtactgg aaacattgct tttcggtatc
     5461 cggccaagcg atggcgtggc gttcgcctgc ggatcaggcg tattgagcgt gacagtgttg
     5521 ttggcctctt tgattccggc ccggcgagcg acaagagttg atcctttgaa ggcgctccgg
     5581 tgtgagtaac ggaagaaggc cgcggcagga actcgttccc cttccgctat cgatcattcg
     5641 aagacaatgg gcttcggctc gacttccggc cggcccggga agaagtgact gtgtggatct
     5701 gtgggtagag gggcaacctc tcgcctccct cgatctgctc gagcgtctcg tgcgcctcgc
     5761 ggtgcaggcg catcgtatcg atgccctcgc aggaaggcaa gtatgcagca agtttgcgga
     5821 ggcccttgcg aagttggcgg attgcgcctg ccggattgag gcgctggcag tgatagaaac
     5881 ctaccgcaag gtgaatgagg ccttgcagga agatgcggcg cggcccgcgt tccggcgtcc
     5941 atgcctcttc cagcacctcg tggcattgga agaactcacg gtcattgaac agccggattc
     6001 ccctccgcag cagttcgtcc atcacgcctg tgggatcggt ggttacatga gttcttccac
     6061 gaactgccgc gcgatgtccg ctggaactct gacgtcccgg tgtgccagcg gcccgccctc
     6121 gtccagaatg ggttccagtt ggtggagcgc aggcatcgac gaatcctcat aaaacacacc
     6181 gatcgggatc tcgtcgcccc atagctgcgc ccgctgcatc gccgcgttta agtccgtcgc
     6241 atcgtaggaa ctgtccaact cgagtttctt cacgcgatcg cggaaccagg ggtacgtgtt
     6301 gtcgtggttg taagtcacac acgggctgaa cacgtcgatg aaagagaaac cggtgtgctg
     6361 gagacctttc ttgatcagct ccgtcaggtg cttcggctcg ccgctgaagc ctcgcgccac
     6421 atacgtcgct cccgccgaga gcgccagcag gatcgggttg accggtcttt ccacgctgcc
     6481 aaaaggcgtg ctcttggtct tcatgccgag tctgctcgtc ggcgacacct gtccggtggt
     6541 caacccgtag atctggttgt ccatggcgat gtaagtcagg tccacgttcc ggcgcatcgt
     6601 gtgcatgaaa tggttcccgc cgataccgaa accgtcgccg tctccgcccg taatgatcac
     6661 gtgcagctcg tggttggcca tcttcacccc ggtggccacg gcgagcgccc tgccgtgcag
     6721 cgtgtgcatg ccgtacgtgt caatgtagcc cggaaggttg gaagaacagc cgattccact
     6781 gatcgtgacc acctggtgtg tcgggatatt cagctcggcc agcgctgcct tcaacgacgc
     6841 gagcactcca aagtcaccgc agccgggaca ccagtcggga tcggccttgc ctttgtaatc
     6901 cgtgattttg aactgcttcg ggacagactc agtcgtcgcc attctgctct ccttatacgt
     6961 tgatctcatg catggggacc gacagtttgg ttccgcgggc tagctgttct ttcacggctt
     7021 ccacgatgtg gtgcggcata tagggctccc catcgtactt ccggatgtgc ccgtcggcca
     7081 cgaagctcgt ttcgctgcgt aagtagcgtg cgaactggcc gctgtaattg ttctctacga
     7141 taatggtgcg gcgcgagccc cggaggatgt cgacaatcgc ttcgccgtgc agtgggacca
     7201 gccagcggat ctgaaggtgg ttcgcggtaa tcccctcctc cgcgaggatc tcgatcgcct
     7261 cctggatgac gcctttggtc gagccccatc cgatcagcgt tacgtcggcg tcacgcggcc
     7321 cgttcaacac cggcgggggc accgcggcct cgattccggc caccttccgc tgccgctttt
     7381 ccatcatggc ccgccgcttg acggtatttg tgaactcgtc gctgatcagg acgccgtctt
     7441 cgtcgtgctc gtcggtggcc acggtgtgtg tgtggccggg cacgcctgga atcgcgcgcg
     7501 gcgagatgcc gctttccgtg atgaggtaac gcttatagct tccgttcgca ccgttcggag
     7561 ccgtgatcag ctcgccgcga tcgatcggga cctcgaaatc aaggtcggcg ggatccacgc
     7621 tcgtgtgacc ctcggacaag aggaggtcgc tgagcacgat ggcgggacac tggaagcgat
     7681 cggcaagatt gtgcatctcg gggatcagct tgaaacagtc gagaatgtcc gtcggcgcgg
     7741 cgattacccg cgggtaatct ccgaacgccg cgcccagcac ctgccacagg tcgccctgtt
     7801 ctgtcttggt cggaacaccg gtggaggggc ccgcgcgctg cacgtcgatc accaccaccg
     7861 gggtctcgat catggcggac atgccgagcc cttcactcat cagcgcgaag ccccccccgg
     7921 atgtcgcgca catcgcccgg acaccggcat gcgccgcccc tatggccatg ttgactacgc
     7981 cgatctcgtc ctctacctgc cgcaccatga tgcccgcctt gcgtgcgtgc gccgacatcc
     8041 aatgcagaac gccggtggat gggctcatgg gatacgcgca gtagaacttc acaccggctg
     8101 cggcgccgcc catcgccagc gccatgtttc ccgtgagcac ggcgtaccgg cgttccgtca
     8161 tcggcagcgg tcgctcaaac ggtccgaaat gctgggtagc gtattggtag gccgcccggg
     8221 cgacgcccac gttctccgcc accaccgcgt cgcccttctt cttgaattgc tgcgtcagga
     8281 tttcctcgag caactggaat ccgacgccca tcatgctcag ggcggctccc actgcgaggg
     8341 tgttctgttc caccttggcc cggctgttcc cgctcagctc tcccaccggc agagggcaca
     8401 gccgcacccc ttcggcggcg tctccgggct tgacgttgtc cccgttgtag atgacagccg
     8461 ctccggggcc gagcaaccgg agatgccggt ccatggtgtc ctggttcagc gggatcaaaa
     8521 gatcgatccg gtctccgaaa ctggtgacct tttcgactcc ggcacggatg gtcaggaatg
     8581 tgtgtccacc gcggatgatg gattgatagg cgttataggc gagaaggtgc aagcccctgc
     8641 gggcgagaat cttagcgaag atgttgcccg gcgtcgcaac accctgcccc gccgcccctc
     8701 caatggcaac agcgaaagtg tctttcatag caatgttccc tcccggcgct atcaggcccg
     8761 aagcccaaac atccgggaag tcccagttct cgtaaccttt gattcaagtg tacccttaaa
     8821 ggcgttgcgt tggaaggaga aaggccttcg gccttccagg aatgcgtctc ctcggcatcg
     8881 tcattgcttt tcgtagactg ctacgtttct catcttttgg cccttcgagt ttgtgcccgt
     8941 aatcgtcaac gtcatctcct ttccgtcctt cgacaccacc cgccgggcac tggataccac
     9001 gttgccggac ttcttcaggg tggcctgggt ggtgcggtcg ttgacccgat ggagcgtgat
     9061 cgtatcgaat tcgtccgaac cgctcaccgg atactctttt ccgtcatact tggctgtgta
     9121 ctcaaacgag gttttctttc cttccgcgtc gacgctttca cctgtccgct ttatgccgtc
     9181 ggcggtttcc tcataggtga tcgtggcgct catgggtggc gggccgggac tgtacttgga
     9241 cttcgcggtg ttgagcatcc aggttccgat ggcgggatcg gctgcccagg cggtgacacc
     9301 caaggccaac attgacagaa atcgtagagt tttcacggac tcccctctct ttcggccggt
     9361 ctgcagctag gcttcaggac cgagggtaat gttgcaaacc agttgcagag catcgtagca
     9421 ccttcaggtt cctgccggga agagcgccgc gttgccgcgg cccgccttcc gtggaacaac
     9481 cgagacgtct ttgcggctac tcgctgcaca aagccgtggc tgggtccagg cgggcggcgc
     9541 ccaaggccga ccggatttcg aactccagag ttccctcggt caccgcgtgc gacatcagcc
     9601 catatgtgcc gatgagcgca agcagcagcg ccatcatgag gccgctcaca gacgtatcct
     9661 ccgagactcg attacagata cgcagatctc taaattacag ttccaggctc attcttgaga
     9721 tacgccgagt cagttaggcc gcggccggta taatgccttc atgaccggcc aggtgttttg
     9781 tgggacctct ggcttcgcct tcgcttcctg gaagcctgat ttctatcccg agaaacttcc
     9841 agccaagaag ttcctcagcc actacgcctc gcgcctgaat gccgtcgaga tcaactacac
     9901 cttccgccgg ctgcccgccg cgacgacgct cgagaactgg gtagccgaaa cgcccgcctc
     9961 gtttcggttc gttctgaaag gccaccagcg catcactcat ttcggccggc tcgttgtctc
    10021 ggatttctct cacctgttct tcaaagccat cgattcgctg cgcgtacaga agcgtctcgg
    10081 cccaatcctg tttcagcttc cgccgaacct tgccgccgat cccgatctcc tgaagcgctt
    10141 cctcgacgcg gcgcctgacg atctgcgcct cgcattcgag ttccggaaca agtcatggtt
    10201 cgccgacccc gtgtaccgca tcctaagtga ccgccgcgcc gcgctgtgcc tcgccgagtc
    10261 cgagaagctg acgacgcccc cggtcatcac gtcggacttt gtatacttcc gccttcggaa
    10321 acctgactac acgcccggcg aacgccgcga gatcgccgca aaagccgccg agctgaggag
    10381 tgatggcaaa gacgtctttc tgttcttcaa gcacgaagac acgccggcgg gcgcgctcta
    10441 tgcggaggag ttgcttcgct caaacgctta gcaggcagtt acgtgcagcc agcgttggcg
    10501 tcgtcaagag acgctgatgt ttctgtgaga caggacgaga tctctgacca atactccgtc
    10561 cctcgaaatc gaaaacgagg atcagatcgg cgttttcaca tgaaaaataa ggacgatgcc
    10621 gccgctcacg agcaatttgg gaccgttcgc cctgacatct caccagcctg agggagatga
    10681 gtgaacattt tgggcctgcg cgcgtcttta atcatatgga aagccgaatg ccaaaggagg
    10741 gtgcccgatt tgcaacattg cgagagacct acgaccaggt gttccaccac ctcgcgcaac
    10801 aggtccgcga gctgcaatcc ctgatgagcc agccggcgac agacgagagg gccgtcgagg
    10861 aggcgaagcg tcgcgtcgag caggcacaag caacgtatcg tgagacccgg gacctgctgg
    10921 catggtccct gctattcggc gacgtgaaaa gcgcctctgc cactgacctt gaagcgctgg
    10981 cccgggcagc ggcaatcggt attggacccc tcggccgcgc gggaggcttg gatcacagat
    11041 cccaagtgga gcaattggct caccaactct gggagcagac cggccgaccc tctgggaagg
    11101 ccgaagagca ctggtacctt gcggagcggc tggcggagcg gttgattcac cgcgcagcct
    11161 gattacaacc ccattcgttt tcagagtctt agcaaaatca acgggcggcc gggaagccca
    11221 gattccggtt ccattctttc gctctgtctc gttcattccg ttcggcgcgg ccaggaagcg
    11281 ccgcggttca accggccccg tttgatatca tgaaaatcac tccggaaaag tccatgattg
    11341 tggagacgca gcgtgtccgt ttcgactcgg tgtccctcga ctgtggtgcg gcgctcgccc
    11401 cggtcgacgt ggcctacgaa acctacggcc agttgaacgc cgccaagtcg aatgccatcc
    11461 tgatcgtcca cgcgctgact ggcgatgccc atgcggccgg tgtcggtcac gacgccggta
    11521 agcccggctg gtgggacagc atggtcggcc ccggcaaggc cttcgacacc gacaagtatt
    11581 tcgtcatttg ctcgaacgtg ctgggaggat gccaggggac caccggcccg gcctcgatca
    11641 acccggtgac cggctgcccg tacgcgatgt cgttcccagt gatcaccatt ggcgacatgg
    11701 tccgggttca gaagatgctc gtcgaccacc ttggcatcga gcgattgttg tcggtgagcg
    11761 gcggctccat gggcgggatg caggccctcc agtggacggt cgcctatcca gatagtgtgg
    11821 tctcggcaat tcccatcgcc agcacggccc gccatagcgc ccagcagatc gccttcaacg
    11881 aggttgggcg ccaggccatc atgtccgatc cggactggaa tcagggcgat tactacggca
    11941 agaaaccgcc ggctcgcgga ctggcggttg cccgcatggt cggccacatc acctacatga
    12001 gtgacgagtc gatgcgggag aaattcggcc gccgccttcg caacaaggac gcctacggct
    12061 tcgacttcga gatcgacttc gaggtggaaa gctatctccg ctaccggggc tcacgcttcg
    12121 tcgaccggtt cgacgccaat tcctatctct tcatcactaa ggcgatggac tacttcgacc
    12181 tcgccaacgg cacggtgtcg ctcggtccag tgatggagac caccagtgcc agatttctgg
    12241 tcatcagctt cacctcggac tggctgtacc cgagctacca gtcgcaggaa atcgttaacg
    12301 cgctgcgcag ccgcaaccgc gacgtggcct actgcgaatt gccctctaac tacggtcacg
    12361 acgcctttct ggtggatgtg gccgagcaga cggagatcat ccggggcttc ctggcgagca
    12421 cgcacaagga gaaggccgga tgacaacgct ggttcaggcc aagcgaaagc catttgtccg
    12481 cgaggtcctg ggccgcagcg atttcgccat catcagcgag ctggtcgaac ccgacaccaa
    12541 ggtgcttgat ctgggatgcg gtgacggcga gctgctccac tggctggcgg aaaacaaaag
    12601 cgtcgatgcg cgaggcatcg agattaacag cagcctcgtg cagcgggcca tcgcccgcgg
    12661 cgtttcggtc tatcagggag acatcgacca ggggctggcc gattaccccg acggcgcctt
    12721 cgattacgtg atcctcagcc agacgctcca ggaaacccgc cgcccgctca aggtgttgcg
    12781 cgagatgctg cgcgtgggcc gccggctgat tgtcgccttc ccgaatttcg gccactggcg
    12841 ggtccggctg gcgcatctgg tcagcggccg cgcgcccaag actcgcctgt tcccgcacca
    12901 ctggtacgaa tcaccgaaca tccacttcct cacggtgctc gattttgagg aactggcaaa
    12961 acaggaaggc tggaccgtcg agcgccgcat cttcgtcacc ggccactacg tcggtaggct
    13021 ggtccccaac ctgatggccg aagtcgccgt cttcctgatc cggaagtagg cccacgcgcc
    13081 tcgtcttcgc gctcagtccg ccgatgacgg cgaaagttcc accaggttgc tcggcgagtt
    13141 gaggaccgac ttcgcgtccg catcggtgaa ggtctcgatc ttcgccgaga tgttcatcga
    13201 acagaacttc gggccgcaca tcgagcagaa gtgcgcatcc ttgaagccct cgtccggaag
    13261 cgtctcatcg tgcatcgccc gcgcagtctc cgggtcgagc gacagctcga actgccggtt
    13321 ccagtcgaag cggtaccgcg cgcgggagag ttcgtcgtcg cgctcgcgga cgcccggacg
    13381 gtgccgtgcg acatcggccg catgcgcggc gatcttgtag gcgatgatcc cggccttcac
    13441 gtcgtcccgg ttcggcagcc ccaggtgctc cttgggagtc acgtagcaca gcatcgaggc
    13501 gccataccag ccgatcatcg ccgcgccgat cgccgaggtg atgtggtcgt agccgggagc
    13561 gatgtcggtt accagcggtc ctagagtgta gaacggcgct tcgtagcaca tctccatttc
    13621 tttctcgacc tgaagctgga tctggtccat cgggatgtgc ccggggcctt cgatcatcac
    13681 ctgaacgtcg tactcccagg ccttcttcgt cagctccccg agggtcttca gctccgcgaa
    13741 ctgcgcttcg tcgctcgcgt cggccacgct ccccggccga agaccgtccc cgagcgagaa
    13801 ggagacatcg tatttctgga agatcttgca gatgtcctcg aagcactcgt agagcgggtt
    13861 ctgccgatgg tttttcacca tccactcggc cagaatcgac cccccgcggc tgacgatgcc
    13921 ggtgacgcgc cgggttgtca gcgggatgta ctgcaccagc acgccggcgt ggatcgtcat
    13981 gtagtcgacg ccctgttccg cctgctcctc gatcacctcg agcagcaacc ccggagtcag
    14041 gtcctctacg cggcgaacgc gcgtgagcgc ttcgtagatc ggcaccgtgc cgatggggac
    14101 gggtgagttc ccaatgatcg ccttccggat ggccgggatg tcgccgccgg tcgagagatc
    14161 catcaccgtg tcggcgccgt acttgaccga ccatcgcagc ttctcgagtt cctcctcgat
    14221 gttcgatgtg accgccgagt tgccgatatt ggcgttgatc ttgcacttgg aggcgacgcc
    14281 gatggccatg ggctcaagat tcgtgtgccg gatatttgcg ggaatgatca tccgtccccg
    14341 cgcgacctcc gaacggacca catcggcggg gatcttctcc agccgcgtga cgtgctccat
    14401 ctcttccgtc accacgccct ggcacgcgta ccgcatctgc gtgcgcacgc tgtctttgac
    14461 acgtctggct acccattccg atctcatagc tgtctcctgg ggtcttgccc cgagtggtta
    14521 cctcgattat ggcacggagc aagactgggg acagaccgac tctgccctga gcgttttgcg
    14581 agcgggggac agactactct gccctgagcg tttgcgagcg ggcagagctg tctgtcccgc
    14641 ttttgcctac gccggtttct cccctcaccc cttccacaac gccgtcagcg ccgaaaatcc
    14701 cgtcggccgc agcacatcca ctcggatccg gcgcggagcc ttctcctgcg cgctgaaatg
    14761 gagcgtggcg ctcttgccag gcgccagttc cggcagcggc gcttcgccct gctccatcgg
    14821 cagatcgccg aagccgtaga caatccaccg cagcctgtaa ccgcgcagcg tataggctgg
    14881 cagcgtgccc cttgcggtca cgacggcggt catcgaggag gccgtgccgc tcagcgtcag
    14941 tgcggcgacc gggctggact cgcttcgcag cgcctcgaac gacggtttcc tctctccgta
    15001 cagatctaca acgccgtgga cgcgctgctt cagcggtccc tcgcccttgt cgccgatgtg
    15061 cgtgcggtag tcgttgtagc agaagaaaat cgccccgccg acccaatcgt agtcccgcac
    15121 tacatttgtg tgttcgcgca tcacccgaat gcgcttctcg tcgccgcccg tgtgctccgg
    15181 acggcactcg cagagaccgt attccgagat gaccaccatc ttgcccggga acacgcggtg
    15241 aatctcctcg agatttcgcc gcatcgcggg cacgtcgccc tgcatccagc tttcgtagta
    15301 ctcgttccac gagattaaat cgagctcgcc cgccacgtcg cgaccgatat tgctttgcag
    15361 cgaattcgag gcgtaggtca gcaggcgctg cgggtctagc ttccgcgcct ccgccgccat
    15421 cgcccggatg aacttctggg cgggcgggtt ctgcccgttc acctcgttgc agagccccca
    15481 cgcgaagatc gacgggtggt tgcggtcccg cccgatcatt tcgcgaagct gctccaggcc
    15541 gttctcgaga atctcgtgcg aaggcgtgtc gcccatcccc tcgaacgtct tcgtccccca
    15601 cgacggcacc tcggtctgaa tcaggatacc gtgccggtcg cagtaatcga gcacgcgccg
    15661 gtcctgctgc cagtggacgc gcgtgaatac gcagttcagc tccttcatgt cgttgtggtc
    15721 gtgctcgatc caccaggcgg gttccgccat gccgaactgc ggctgactcc ccgccatgcg
    15781 ttcgacgccc atcaaccgga ctcgctcgcc gttcaggaaa agaccgccct ccttcacctc
    15841 gatacgccgg atgccaaacg tgtccgacag ttcatgaaca ggcttgcccg ccgcctcgat
    15901 ccgagcagac aggcggtaca ggttcggctg atcgaagtgc cagagtcggg gctccgccag
    15961 caccgcctcg ggaagcgaga tcgtctcggt ggcgccaggc cgcacagcca cgcttgccgc
    16021 gttcggttgc cgcaggacca cgcggccgct cgcctcttcc gccgcttccc agccgattga
    16081 aacgcccgct ggaaggccgc ccgcgttccg aatcaccgcc cgcacacgga tccacgcgct
    16141 tgcttccccc ggctccggca tggcgtccac ctcgatgcgt tgcagaaaga cgggcgatgt
    16201 caccagcagg ttaacgggac gcgtgatacc gccgtccgga gtccagtcgt aggagtccac
    16261 gcgcggcagc atcgctgggt cgaacgaatt gtcggcacgc accgccagaa catttcgttc
    16321 accgtacttc agacccgctg tcaggtcgag ttcgaacgcg gtgtagcctt tgcgcaagtg
    16381 gctcccgacg ggcttgccgt tcagccacac gctcgcgctg tgatacaccg cctcgaattc
    16441 cacccgcacg tgactgcccg cccactgcgc cgggacgtcg aactctcgcc ggtaccacgc
    16501 cacgccaagg tgcgcgtgtg actcctgtgc tacctgccag gtgtgcggaa ctgtgacctc
    16561 gctccaaccc tctcctcccg ccgtcggaag gtgccatccc gccgattcgc cgcccttgcc
    16621 gggatccaac cggaaccgcc acgtgccgtt gagtgacacc cgcgctccag aagccagtcc
    16681 cccggcggca ctccccgccg ctcccgccgc agccggagcc gcagccgccg cactttgcac
    16741 gaaccgtctt ctcgtcaacc cgctcatgtc gtcctcctcc gcagccaaac atcataccgt
    16801 cgtcttttcg cctgccgggt tacaatgact ggacatctgg gagcagtaca tggaggaggc
    16861 aaggtgctgt caaagaactg ggaccacaga aaacccttgt atgatttcat cgtcgtcgga
    16921 tcgggctacg gaggcgcgat cacggcggcg cgaatcgctg ctgcaaacct gaatccgaag
    16981 cccttggtct gcatccttga gcggggccgg gaatggccgg tcggcagctt tccagacacg
    17041 atcgagggga tcgccggtga gattcgcagc gatatcaacc ctctcggcct gcacgagttt
    17101 ctcacctatc gcgacatctc cgtgtggaaa ggaaacggcc tcggcggaac gtcgctcgtg
    17161 aacgccaacg ttgctatcgt gcccgacgcg gaagtcttcg actcgctcgg ctggccgaaa
    17221 tcgctgaacc gcgcggagct catgccctac taccagcgcg ttcgcgagac gctcgatgcc
    17281 aatccccacc cgaacgctct cggccttcca aaagtgcagg cgctcaagcg gcgcgccgac
    17341 cagatcggcc gcgaggtcct tgccctcgac atcgccgtga acttccaccc cgacgggccg
    17401 aacaagcacg gcgtcccgca gaagaagtgt accaactgcg gcgactgcat caccggttgc
    17461 aacgtcggcg ccaagaacac cctctacatg aactacctgc ccatggccgc cgatgcgggt
    17521 gcggaaatct tcacgcagtg caaggtggag tgggtggaaa aactcaccgg cggaggctgg
    17581 aggattcacg gccgccgcta ccgcagcaag agccgcaccg agaagttcac gttcgacgcc
    17641 gccaatgtca tcctgtccgc cggttcgatc aacaccaccg agattctgct ccgctcggag
    17701 atgcacgccc tctccgtctc tccccgcctc ggcagcggct tcagcggaaa cggcgacttc
    17761 ttcgccatcg cttacaacgg cgactaccgc acgcaggtgc tcggctttgg caatcacctg
    17821 aaccatctgc gtgcgtccag tcccccgggt ccgaccatcg tcggcgcggt ccgctacaac
    17881 accggagtgc cggtggatca gcggatcaca gtcgaggacc tctcatttcc gaaagggctg
    17941 gtggaaggag ccgcgcggtt gttcctcaca caacccggtg tcgataccga caccggcgac
    18001 gagactcagg aacgtgcgcg tgccctgcag gattccgacc ccttcgccga aatccaggcg
    18061 gacggcgcgc tcgatcacac catgatgtac ctcgtcatgg ggatcgatga cgctcgcggc
    18121 tcgatggtct tcgagaagac gctgttcgag cccgatggcc gtatgcgcat cgagtgggac
    18181 gatgccggcc gccaagttgt tttcacgcgc atcaacgaag aactccgccg gcactcccgc
    18241 gctctcggcg ccacgtttat cgaaaatccc acgtggagct tcctgaatct ccgccatctc
    18301 gtcaccgccc accccatcgg cggctgcccg atcggggagg accacatcca gggtgccgtg
    18361 gacgagttcg ggcgtgtgtt cgcgggcgac ggctccgtgc acagcggcct cttcgtcgcc
    18421 gacggatcgc tgctgccctc ggctctcgga gtgaatccgt ttctgacgat ttccgcgctt
    18481 tcggagcgca tcgccgagcg gaaagtccgc gagatgcagg gtgaagcgta tccggcgccg
    18541 aaggtgtccg tcagtctttc cggcatcgac ccggtggagg cggccggctg gaaagaagcc
    18601 gaactggagc ggttgttcgg gcgcgcggag tcacagggca tcgacgtgat gctcaacagc
    18661 gggaaacgcg agatcgacgc cgccaggagg accgtccata acgacgaata ctggaagggc
    18721 ttcttcccgc gaggtcacat tctcaacgcg atgtccgccg tgattttcac tgggttcaag
    18781 aagcgcttct ttaagcaggg gaagaagtac gccggcgtca ccagcgacac cgacggccgg
    18841 atcaacgctc gcaattcgct cgaagagctg aacctcaaaa agcagaccgg agacctgaag
    18901 ccggggcgat acattctcct ccgttacgtc gatcctccct ggcagggcta ctacgacgtg
    18961 ttcaaagtga tcaacgagaa cctgctcatc gggcgcgtct acttcggcga gtacccgaat
    19021 ggcattcgca tgttcacttt cccgatgacg cggtcgtacg gcttctccgc gatgacggtg
    19081 gaggaccacc ggcagttgtt cgcaagcgga accgtgcccg cgaaggaaga gctgaacggc
    19141 gcctggcgca tggacgtcat ctcgaacgcc aaccaggccg gtggcctcgc gtggttgagt
    19201 ttcgacctga aaccggacgg ccgcctcgaa agccgctacc atctcatggg gctgatggag
    19261 ggcctcgtca ctccgacttt cgtcgccgat catttccggc tcgacgactt caccgatttc
    19321 cacgatgaga tccgcaagat cgacaacgat ctcttcattg gaaaatacgt cactgagttg
    19381 ccggcggccg tcgctgggct gttgccagcc tcgtcccttg gccttctcca cgtggaaggc
    19441 gacgaccgca agcggtttgg catgtattac ctcctcacgc gcgcagagaa aacagacgtg
    19501 ccgacgacca cgcttttgcg gccgctactg gagacccacc tccccgatgg ccttggcatg
    19561 gtcttcgacg aggagatgga gggatggtac tcccccgggg tgttcaccag cggtccggga
    19621 cgcaagggcg atctcgagat tgccgctcgc ataccggatt caggcaagcc ggcaggaggg
    19681 tctgactgcc gcttcgacct ccgaatgacc gtccgcgatc tcaacgagtt cattgacggc
    19741 gcggagcacg aggcgcgcgc gtcgggctcg atctctttcg aacagtttga aggccgcgga
    19801 ccggtgacgt tccccgtgga cgaccggcgc agctacttca actacctgcg catcaatccg
    19861 gccaccggcg aggcggagat gcgctatcac ctcgaattcc gcagcgacga cggccgtgcc
    19921 tttacgttcg agggccgtaa gtacatgcaa aaggacgaca gcggcggccc gcgggcgatg
    19981 cgcgaagtgc ttgacgacta caccacgctc tactgccacg tctatgagga cggcacacag
    20041 gggcggcgcg agctcggaac cggctatctc aagttccgga ccttcgagga tattttcgcg
    20101 gtgggcaacc tcgccggctt cctccggtca ttccaagtga ccggcacgga cgacccgctc
    20161 ctccagcttc gcggccaggc gcgcttcctc gccttcacgg gccagttcgt ccagcaggag
    20221 tacgacccgg tgatctcatc ggtccccgac tgagtgaacg cctgaagacc ccggtttggc
    20281 agccgtcacc acgtagtatg gcgtcgccgt ccgcggatcc ggatgaaacc agccgccgcc
    20341 ctgcttgtcg agcacttcga agtagtacag caggtcgaag tccggggtca ggtcttcggg
    20401 cggtatcgtg aacacagccc gcccgggcgc agcctcgagc gtcttgaatt tcgcgagttg
    20461 attcagcggc cggtagtgca accgcaccga tttcactgac gcggaagccg tcctgcccaa
    20521 ggccaccgac aaaggcctac ccggcacgaa catgtgcggc ggtatgtgcg tgaacaccgg
    20581 acgcggcatt ggcttcggtg cggtgaggac cggaaacttc ggcgccgctc ccggcacttt
    20641 caccaccagc ccgctgacat tccactcgcc ttccggaaac gcgatatcga gcacgccgtt
    20701 cgtcgctgtg ccgctaacct cgctcacttc cccgcccgca ttgaggaaca gcacctggcg
    20761 ctctccattc gacgccgtcc gcactcgaaa cacctgccgc ccgctgccgc gtatcgaatc
    20821 gccggtcaaa gcgtcgtgag gcagcggacc cggcttcgtc accaccgccc gcacctcgtg
    20881 atacgtcgca agccgcgccg ctaccgcctc gcgctcacct tccgtcgccc atccgtagcc
    20941 tcgctcctcg gaatacgccg tccccggatc gactggaagg aaacggggct ccaccgtatt
    21001 catccagacg tacggatcgt tccggtagga tgacccgtgc ggccgcgtca ccggtccccc
    21061 gaaatcgaag ccgaactcga actgtccgaa ccgttcgaga actttctccc gctcctccac
    21121 aatcttcaaa tcgtgccgga catacgcgag tttgtgcttc cagtgcccga cgtcgtcggg
    21181 accgaacgcc atctcctccg ggtagagccc gtctgtcagt cgcgccaatc cttcccaaac
    21241 ctcagccgcg gccgacagct cgcgcttcgc atcggccagc gccgccggat ctcctgtctg
    21301 gtaaaaccac tccagccggt acgccgcggt ctgtttccgg gcgtggtagc gagcaagcga
    21361 tgagaggacg ctgaagtcgg gttcagagcc tgaccactcc cggtgctctc ctctaagcct
    21421 gcttcgcgcc cgtgcaactg cctgttctgt ccgcgcggca agctcgtcca ggcgttgcgc
    21481 ggtatcgagc gccgtctgct ttgcggagac cattccctcg atcctattcc ggactgcctc
    21541 cggaatccct gcgatcatcc gccagtcgct tggccgcacg tccttgtaag cgtcgatcag
    21601 cccgcccgga ttgatctccg gccagatgta catgttcgga tccgccaggt gcgcggccac
    21661 gatttccggc aacacgccgc tcgcgccccg gtaggcttcg agcacatccg ccgccgccgt
    21721 tccgaaccgg cgcttcagtt ctgccatcca tgcgctgtcc ggagtcttcg ggtcataact
    21781 caggcggccc cacagaaggt agaacagcca gtaacgctcg aactcgtgct tccagaagat
    21841 ccggttctcc cgcccggctg cgaacacgcc ccacttccca gggcggttcc cgaaaccctt
    21901 ctgtgccatc ggcggatcga tttcgaaccc gatcgaatcc gaaaggcgga acgtcggcgt
    21961 catgcggcgg acgaagtcag gatcgcccca cagcagcagc cggtttgacc cgagtcccca
    22021 gacctcccag tagaactcgt acggcctcgg tttcttcagg aagtcgagat agctgtaccc
    22081 cggccacgtc tccgccggct ggtacgggcg ccctaggtcc tccgcccaat acttcgacga
    22141 cagtcgcagc ggcgccccgg atgtaaccgc ggcatcgagc atacccggct gcatcgccca
    22201 accgcgcatg tctaatgtca cgcgtcggcc cgcttccgca acggcgcgga agacccaatc
    22261 ccggtagaac tccacctgct tgtcggaggg aatgccgctt tcggaattgg tgcgcatctg
    22321 gacgctacgg atcgcggggc aggcggcgag gaccttcttc agtgcggcgt aactgtacgg
    22381 gccgatgttc tcggccgtca gtccctccac ggacggttcc attcccggtt ggatattgtg
    22441 ttcccagata ccgagtgtga agtccacgcc gtgttccgcc gccgtgtggg agatgaagcg
    22501 cagcatctcc aggttccgat ctcgttccgc cgaggacagc cccggcgcgc gaacgttccg
    22561 gaactccggc aactccaccc agtacgggta cggcggcgcg aggtactccg tctggtgcgc
    22621 aaaaaccagg ttgaaacggt tgaaacggtt ccgcgccagc attgcgaaga agtcccgcca
    22681 gtattcgtgc gagtaatacc agtcccgctc caggtcggcg ttgtgcagga accatcggat
    22741 gccccgcatc ggcgtcgcgg gcgcgccttt cacggcgaat aaccgccctc gctcgcggat
    22801 ctgcgccgcc gcttcgagca gtccgtacat aaggccgcgg aggtcgccgc cggtgatccg
    22861 gccgggctcg attcgataag tctccggcgc gtccggcgta atttcggcag tgaaccttac
    22921 ctgactcggt ttcagtccgc gctcggcgat ggcgcgttcc acttcgatcc tgccaaactc
    22981 aaccggtccg caggcggcgg gagaagcgca gatcaggcag agtagcggca accaggctct
    23041 gtttcgcatc gcggaaacgc tcattatatg tgacgcggga gctacaatat ggtccggaag
    23101 gaatcgtgcg ggaggtacat catgcccgtc tacatcttcc tttgccggga ctgcaagaaa
    23161 gaattcacga agatcctccg gatctcggaa ctggaacagg gcggcatccg ttgcccccac
    23221 tgcaatagcg agaaggtgga acagaaggtg gctgccttct ccgcggtcac atcgaaaaaa
    23281 agttgacgcg acggccgatc ctcgcttgtc gccttgtaga gccagtggag caggccatcg
    23341 ttttcggtgg cctgctacct ccactcgtgt ggcatgggtc actcgatccg ccatctcggc
    23401 cgtcctaccg gcagcggacg acgcatttaa ccgctccaac gtcaatgacc taacagcacg
    23461 atgacatcgg cgacctacgg gctcaccggg ccttttttct cggatcaaca ttacgggaga
    23521 atgctcgtat gcaacgaaga gtcatgatga gggtcctgat ttctccggtc ttggttctgt
    23581 gcgggctcgg gggcgcgcag acggttcagc attcgacggc gcagctaacg gtcgatggga
    23641 agctgggtga ctcttactgg caggcagtgc aagccaatac cttgatcccg tcgcagagcg
    23701 gcgtgttcgc agatatgggc ggcgatgtgc ggctcggctt tcgcgagggc tacctgtgcc
    23761 tggcggcacg cctgccggag cctgggggca aagtgctggc gcgttcgatc ggacgaaatc
    23821 cggtatggga aaaagatgca atggaatcgc cggaggtgga ggaccggatc gtctacacgc
    23881 tccggtatcg cagcacgggc ggtgcggaaa gaaatgtcac catcgcaatc aacccgtggg
    23941 gtgcgtaccg cgtggaggat cgcggccaag tcattccggc ggcgaaaatc ctgagcgcgg
    24001 cggaggtcac tgggcagggc tggagcgtcg aggcagccgt gcctctcggg atgctggatg
    24061 tcgaacgggc tgcagcaacg caagtcggga ttcgcgcgga gcgtatccgg tcgcggcgtc
    24121 cgctggcgcc ggaattccga tggtcctggc cggcaggcag cgagtacagc aatgttgtgt
    24181 tgccgccgcg aagctttgcg gcttccggac agaacgctcc ggtccttcag ccaccgcccc
    24241 ttggaaatac cgacccgcct ctcgaagtgg gcagggttct gcaagtgccg ccagctgtcg
    24301 ctgagtggga tgacccggcc tggaaaggga tacctgagtt tgagctggcc cggaatgaga
    24361 cgtcgccgcg ttctccgagg cattcgacac gggtcaggtg gatgcacgac ggccgcaagc
    24421 tcgcgctaca gttccgcatg gacgaaccgg aaccggtggt tgcacgggcg ggaggaagag
    24481 atagtgcggt gaccggcgac gatcacgtgg ctgtttattt ggcaacgacc ggctcggctt
    24541 ttctggaaat tgccacgaac tctgtgggcg caatccgcga cgccgtaggc aggggcccac
    24601 actcgatggg aacgcggtcc gactggaacg cggacttcga agtgcaagca aacatccggc
    24661 acggacactg ggtcgcgcgg ctaaacatac ccctcgaaca gtgtgccgcg gcgcttggag
    24721 aaaccggcgt gccacggaac tggcgcatcc ttctggtccg ctaccgagct ccccggccag
    24781 gcgaggcggc ggaaacgagc accctgcccg ggctggaggg agcttcgagc ttctatgggc
    24841 ccattcgata ccaagccttg aacttgctta agggcgatcc gacagaggtg aagctcccgc
    24901 atgcagtcca aaaggtgccg cctgccggtc tggccggcga acttgcggcc atggaaccat
    24961 acgtatggcc gtcgctcgac cgccgttatc aaggcgtgcg tactatgctc cagcgctata
    25021 tgcagatgcg gacggaaggc gctgtgttgg cagagagacg cgcttgggaa caggtagaaa
    25081 cgcgcgagga ctgggaacgc ttccgtgacg agcggatcaa aggactacgc gagtctgtgg
    25141 gcaagtttcc gccagacaga ccgtcgcttg acgtacgcgt gagcgtccgc cagaagggcg
    25201 acggctacgt gcttgagaac ctggcgttcc agagccggcc gggattctat gtgacggcga
    25261 acctctatct tccggagcgg gtcacaccgc agattccagc gatcatcatt gtgcatagcc
    25321 agcactatcc gaaaacgcag ggcgaattgc acgatatggg cgagatgtgg gcacgaacgg
    25381 gttgcgcggt gctcatcatc gagcggcctg gttacggtga gcgggcggag accacgacat
    25441 ggtacaggca ggcgtatggg tcccggttta cgttcacaaa gcaactcttt ctaatcggcg
    25501 aaagctactc gggatgggcg gcgtgggaca tcatccggtc cgtcgatttc ctgcatgacc
    25561 gcccggaggt ggacaagaat cggatcatcc tgcttggttc ggtggcgggt ggaggcgagc
    25621 cggcagcggt cgcagccgcg cttgacccgc gaattacggc agtcgtcccg ttcaactatg
    25681 accaggggca tgtcagggta cacggggact ctcccgggca aatcgccagg cagttctcgc
    25741 cctggttggt ggcggcgtcg gttgcacccc ggaaatttgt gcgggcgttc gagttcggct
    25801 gggagggggc tgaagagccc gactacccgg agttgtgggt ggatggcctg aagcggtctg
    25861 agaaggtatg gggcttttac ggcgcgagag agaacttggc ctcgtcacaa gcgtacggat
    25921 tgatccggct gagcatggag cgggtgagcc actgcttcag catagggccc cagcagcgcg
    25981 agccgctcta cgcaattttc aaccgttggt tcggtattcc gtttccatct cagaaagacc
    26041 tggaaatact gcctgattct caactctcca caaatccggt gcgagaggca gcccgaagac
    26101 aggaagcaca gcggcggagg ccccatgcgg atctgttgag tattcctccg gatctctccg
    26161 caaagatcca gcggaggaag atccaccaga tcgcgcacga gatgggtaag gagatgctcg
    26221 cggctgcacg ccagcgccgg cagtcgctgg gtgccgaaga gagggtgcaa cggttgcggg
    26281 acgagttgca gccgatgctc ggcgacatct cccctaaagg ctccccaagg tcgaagacgg
    26341 tctggacccg ggcgcttgac ggcgcggtcg tagaggcggt gagcctggag gtggaagacg
    26401 ggatccgggt gccgctattc ttgatgcgtc cgcaagggcg tccgatttca cccgtggttg
    26461 tggcgttggc acagagcggc aaggaacggt ttctggcaaa ccgatcgaag gaactggaac
    26521 agttgctgcg ggcgggggtt gcagtgtgcc tccccgacgt taggggtacg ggcgagacag
    26581 cgtccagttc ggaactcgat gatcctgaca cgcagccggc gattcgggag tttgatctcg
    26641 cccgcagtct gcttgggtcc cggctaaagg atctgcggac cgtgctcgtg tatttgcgca
    26701 accggtctga tatagacgcg aagaggattg ctgtatgggg agattcgttc gcacccgcca
    26761 atccgagaaa cttgtggctc gacgagatcg aatttgaggc aggcccccag atacagtact
    26821 gggcggaccc gctcggggcg catctggcgc tgctggtggc tctatacgag aacgacgtgc
    26881 gggcagtggc ggcgcgcggt gggctcgtcg gctatctgac cgtgctcgac gaaccctaca
    26941 cgtatgtccc gatggacgtg atcgtttggc gcctgctcaa gagcgcggac atcgccgata
    27001 tcgccggggc gctagctcca cgtccggtgg tgctcgccgg ccttgtgaac gggaggaaca
    27061 tcctcacaga cgctacggat ctcgaccggg ttttggagcc tgcgaagcgc gcctatgagt
    27121 cggcaggcgc ggcgaaccgg ctgacgcttc gcgtcgaatc ggagaatatg gcagcttggt
    27181 tgattgcagc aacgaagtag cgtcaggatg cggtgctgcc ataccttcgg tcgcctgccg
    27241 tctccgtcac gtaccgggcc atacggccaa ctcctccatt cggtacactt tctaagttgc
    27301 aggggggtcg atgttttcca agatcgttcg caggtcacac atgtacctcg cgctgttcct
    27361 tgtgccgtgg atattgatgt acgcgctcag caccatggcg atgaaccacc gcgagttctt
    27421 taagcagttc ttcggcgagc agccggtggc gtgggagagg gagcgcgagc tgacctatcc
    27481 cggcacgttt cccgaggatg caaagccgaa cgatgtagcc cggcagattc tggtgagcct
    27541 cggcatggac ggggcgcaca atgcgaaccg ctcgaaggac ggcgcccgcc tcaccatcgt
    27601 tcgccacgac ccgatcacgc cccagcgcat cacgtacaca cccgcggatg gccggctcgt
    27661 catcgagagc caggttttcc gcacgccggc gttcctcgaa cgcatgcacc gccgccgcgg
    27721 ctaccagtcg gactatcggc tggacgacac ctgggctttc tcggtcgacc tagtgatcgc
    27781 cgccatgatt ttctgggtgg cctccggcct gtggatgtgg tgggagatga aagtgacccg
    27841 ccgtcttggc ttcgccttcg ccgcgtcggg cgtggcgatc ttcgccctgt ttctgtcaac
    27901 catctgagag gaaaccggcc atgacctttc accttcttac ccgtcgcatt catctctgtc
    27961 tcggcttgtt cctccttcca tggttctttc tttacggcgt gagttcgatc ccgttcagcc
    28021 acgcgcccta tttccagaag ctttacgagg acggagtccc acagtggacg gtccgcttcg
    28081 agaagccgta tgagatccag gtgccgaaag agggcgacat gcgcgcgacc ggagcgcgca
    28141 tcctcgcgga caacgggctc aaaggcagtt tcggaaccta ccggccgaat gagaaggttc
    28201 tggagaccta tctgttcacc ttttggacgg ctagccgggt gcggtacttc accgaggaaa
    28261 agcggttgct cgtcgaggac cggcgctccc gctgggatca gatcctcaca ggaatgcacg
    28321 cccgcggcgg cttcgagcag gattcgattt tgagcgatgc ctgggcgatc ttcatcgacg
    28381 tcgtctgtat cggcatggtc ctgtggatcg ccagcggcat ttacatgtgg tggaagatcc
    28441 gctccacccg cgtctggggc tggctcgcgc tcggcggcgg gttagccgcc ttcgccatct
    28501 tcctggcagc gctgtaggac catcagacgg tgcttagccg aggtcggcga taccaacgaa
    28561 atgaggcagg gcagcgtagg tcttcgccgc gtaagcaagg gcagcccaaa tatcttcggc
    28621 ggtcaatcga ggataggcgt ccaacaggtc ctggacagtc gccccttcag ccagtttgcg
    28681 aagcagaagc tccaccggaa ccggtcccgc ggatgactgg ctttccaagc atgacttcgg
    28741 gattgacctc aacgcgctcg atcatcgcca ctcccagacc gattgtagca ccccgacctg
    28801 ggcaagcttg aaccgggcca gcatagtctg gacaaacctg ccgatgcctg ggggctcctg
    28861 gcggcgctgt agagcgaacg ggacagccgc cgcacgacag ctttcttgcc atgcttcgcc
    28921 caacgtcaca agtggaatac ccgcttcaac aggccgacat actgcggacc agtagtaatg
    28981 atgatcaatg cgaaatatcc caccgcgagt acacccccac cgacgaattg cccccattgc
    29041 ctgagctccg gccgcgttcg aagaaatccg gcgaggcaag cgcctataag caggaaatgg
    29101 cccgccgctg caactacggc gatcaacagg tcaggccttg gaagttcgcc tgttcgaagc
    29161 aaaattgaaa actgctgcac tccggctttg atgaacgtag tcacgaacag gttgatgtaa
    29221 ttccagaatg cggcgatgat gcaccccgca ccgaatcccc acgcgctctg catccgcgcc
    29281 agcacgatca ccgcaacgta gatcacagcc tgaagagcat gcagcactcg gattctcgga
    29341 tcaaacacgg ctgataggaa gagagcaaaa atgaagaatc ccgctccggc caaaatcgat
    29401 gcgttaatcc aaccaggatt ctccccagac ttgagcatca cccagagtaa cgatcccgca
    29461 gctcactgag ttccaggatt tctcgcggcg ctgtcagggg ttgcagccgc agtggtgaca
    29521 ccgggcttca gcctgtgccg gtcgccggcg gtggtcgcta cgcccgggtc gcccgccgtt
    29581 gtgtgagccg gtagcttcgc tcccgccagt ggatgacgcg cccgaacgcg gatgagagaa
    29641 gcgagcccag ccacacccag gtcgtgagcg gcggcaacca cgtgtatacc cacccgtaac
    29701 gccggaacca gccctcgtat cccttgagca cggaccgcgc cagcgccgcc cgccgcgccc
    29761 ctttccacat ccccagtccc cacagagtga ccaccgccac tgccgccgcg agttggccgc
    29821 gagccagttc tatgagacag gccgccgccg ccccgcagta cacaaggtgc gaaatcagcg
    29881 ccatccacca gagcgccggt cggtggaacc gcgtcaacag catttggcgc cgggtccatt
    29941 cgcagaattc ccggaagcct gtgccggcgg tctcggccac gatcgcgccc ggagcgaagc
    30001 gaatctggag tccggctttg agaacggcgt cgctcagtcg atagtcgtcg ctcactgcat
    30061 ccttccagaa atcggcgaca cgcgcccgga cgaagacctc gcggcggatc cccatcgcgc
    30121 caccccacgc gaacctcagt ggtccgggac cgaatcggcc ggcaatcacc gcgttccata
    30181 cgctgcgcag gatcgcccac aatccgcccc tctccggaac gtgccagcga tagccggtcg
    30241 ctgcgccggc gcgatcgtct tgcaaaacct ccaccagcga gcgcagccac aagcgtcccg
    30301 gccggccgtc cgagtcggcg aatgcgaaga tcgcggacgt cggccgggcg gccttcaccg
    30361 cggccatcag
//
LOCUS       DATIFQ010000434         6192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1103559, whole genome shotgun sequence.
ACCESSION   DATIFQ010000434 DATIFQ010000000
VERSION     DATIFQ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103559"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="VLE22_21775"
     CDS             complement(<1..1553)
                     /locus_tag="VLE22_21775"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSB17095.1"
                     /translation="MRRATALFVLGCASSAMLPGRQAEAERKLAEIFSPAALSATHCD
                     LASAKTGILKAVFWKGYATTGFEIKRLPVADWSPWNSFRFDVENPYREPFSVYVRISG
                     RTDHPASETYTGGTFDGFVIGPGRSTVEISLEKMQSPDEKPVDPRRVAYLGVFFQPLF
                     LRDGMDLKFTEDKTFVLSNPRLSAASATLQKQPYGDLLFRETDAGLAARRQEAERALH
                     ELEKRIEEAKAKGVETAYAEIYPFLAGLAFHSRLVAFWQDRKEEQRRALDFLIQGARN
                     ADNELRSAIDGKAPRRSVPPVPEYSTLAAKDGYFRLNGDPILLFGMLYNRQGPLLRWF
                     ANSQTDYGTQLVAGATRHDVERQPIWEAYHKYPDTHRVGWDHSDHIIRDRSSWEVLGP
                     PVNVCLESPHTREAVAKRIETFERSQTGDRDHLAQNLGYEYTYVCYCDYTKRMWEEWL
                     RRKHGEIGAANRIWGTKFGGFAAVPMPRQENAAANRALWFDWASFNLYRFLEQIRWTH
                     DQIRKWEPS"
     gene            complement(1550..3898)
                     /locus_tag="VLE22_21780"
     CDS             complement(1550..3898)
                     /locus_tag="VLE22_21780"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HSB17096.1"
                     /translation="MDRERNTLRYGYLVPALVLILAMTALAQSGGVPVYLDPKQPVEK
                     RVEDLLGRLTLEEKIGQMNMPCVYVDELGKEIPAKLEACRQFTLGTREKGVGPGGGFF
                     TLPNTVIPEGTRRQAEFLNELQRLAREKTRLKIPLLQTEEGTHGLMCSGGTIFPEGPA
                     LGSTWNMHLLSDIYTVAAREARAIGVHQIFTLVVEPNRDPRLGRNQEGYSEDPYLCSR
                     IAETIVGAVQGDNIAAPDKTVAGLCHYPGQSQPVSGFERGAMEISERMLREVFLPPWV
                     AGIKKMGGRGVMATYPAIDAVPVHSSDWILTKILRDELKFQGLVLGEGGGLSTIVYEG
                     IAPGQKEAGVWALKAGLDVGISYEDAYMRPLAENVREGRVPMTLIDRAVRRILRQKFD
                     LGLFESRFVDPERAVRVTHTKESQDLSLRAAREGIVLLKNEKNLLPLKKDIRSIAVIG
                     PNADHELNLLGDYVALKVLQDVVTVLEGVKAKVGRGTRVEYVKGCDVIGNKVNEIETA
                     RRTAKNADVAIVVVGENAWATEYPTNGEGYDVASLDLTGMQEDLVKAVVETGTPTVVV
                     LVNGRPLSIRWIAEHVPAIVEAWLPGEKGGHAVADVLFGDYNPSGRLPVTVPRHSGQL
                     PSYYNFKPSKAYWIKEGWGKRYVDMSPNPLWEFGYGLSYTAFEYANLRIDQPKVPAGR
                     DVPISVEVKNTGRREGAEVVQLYIRDVVSSVSTPVKQLKGFAKVALKPGERKTARFLL
                     TPDDLSLLDRSLKRVVEPGIFKVMVGHSSEDIRLTGEFEAMK"
     gene            3951..5039
                     /locus_tag="VLE22_21785"
     CDS             3951..5039
                     /locus_tag="VLE22_21785"
                     /inference="COORDINATES: protein motif:HMM:NF025957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HSB17097.1"
                     /translation="MRSIALLSVLTFVLCFAQESKPEPAVDWHDVRKFGVEGQGWKDT
                     KHPFDRLPAKAEGVVRPPVWDLSQDSAGLCVRFSTDASIIRARWTLRHENLALPHMPA
                     TGVSGLDLYVRLSSGWHWIANGRPLKQTNEQTLVGNWPGGKREYMLYFPLYNGAESVE
                     IGVPPGVAIEPGPPYRPGVKPILFYGTSILQGGCAARPGMAYPSIIGRMFDWPTINQG
                     YSGNGKSEPEIATLFAEIDPAVYVYDSLPNLNPAEAKERVEPFLRTLRKAHPRTPIVL
                     VENVIYTNIQFSEQRRTEVTEKNATLKAVYQKLRKQGDKNLYYVPAAGLFGSDGEATV
                     DGVHPTDLGFLRMAQTIAPVLKPLLRKR"
     gene            5057..>6192
                     /locus_tag="VLE22_21790"
     CDS             5057..>6192
                     /locus_tag="VLE22_21790"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB17098.1"
                     /translation="MMRPTRREAVTALAGITASCTRREPAAEPPKKAAPLSLSEYAPK
                     SMLVVPETKVEKARYPVVDIHTHISWLTAEGLGHQLALSGKRYYLAPAEEIVKVMDGL
                     NILGMANVTGGYGASLQEGVDNYDRRFPGRFFTLVEPMFDQVNQSGYATMQGDAVEEA
                     HKQGAKGLKILKSLGMYLRENVTEGPLIKVDDPRFDPMWEACGALRMPVAIHVTDPAA
                     FFYPADRFNERYEEMHQHPDWDFSGKDYPAKMEVLEARNRVLARHPKTQFLGLHISNW
                     PEDLGLVSQWMDKYPNLWVEIGARIAELGRQPRMARKFFEKYQDRILFGTDIFARGKM
                     AADMASLGRIYPIYYRFLETEDEYFNYSPGAVGGSGRWRIYGLGL"
BASE COUNT         1118 a         2003 c         1900 g         1171 t
ORIGIN      
        1 ttcgaaggct cccacttccg gatctgatcg tgcgtccagc ggatctgctc caggaagcgg
       61 tagaggttga agctcgccca gtcgaaccag agagcgcggt tcgcggcggc gttctcctgc
      121 ctgggcatcg gcacggcggc gaacccgccg aacttcgttc cccagatccg attggcagct
      181 ccgatctcgc cgtgcttccg gcgcagccac tcctcccaca tccgcttcgt gtagtcacaa
      241 tagcagacgt aggtgtactc atagccgagg ttctgcgcaa ggtgatcgcg gtcgccggtc
      301 tgcgagcgct cgaaggtctc gatccgcttc gccaccgcct cgcgggtgtg ggggctttcg
      361 aggcagacat tcaccggcgg cccgaggact tcccaggagc tgcggtcgcg gatgatgtga
      421 tcggagtggt cccagccgac gcggtgcgtg tccggatact tgtggtaagc ctcccagatc
      481 ggctggcgct ccacgtcgtg ccgcgtcgct cccgccacca gttgggtgcc gtagtcggtc
      541 tggctgttgg cgaaccaccg cagcagaggc ccttgccggt tgtagagcat cccgaacaga
      601 aggatgggat cgccgttcaa gcggaagtaa ccgtccttcg cggcgagcgt cgagtattcc
      661 ggcacgggcg gtacggagcg cctcggcgcc ttgccgtcga tcgcgctcct gagttcgttg
      721 tcggcattac gcgctccctg gatgaggaag tccagcgcgc ggcgttgttc ctccttccgg
      781 tcctgccaga acgcgaccag ccgcgaatgg aatgccagtc ccgcgagaaa cggatagatt
      841 tcggcgtagg ccgtttcgac gcctttcgcc ttggcctctt caatcctctt ctccagttcg
      901 tggagggccc gctcggcctc ctgacgccgc gcagcgaggc ccgcgtcggt ctctcggaac
      961 agcaggtcgc cgtacggttg cttctggagc gtggcgctcg cggcgctcaa ccgcggattc
     1021 gacagcacga aggttttgtc ttcggtgaat ttcaaatcca tgccgtcgcg gagaaacagc
     1081 ggctggaaga agactccgag gtacgccacc cgccggggat caacgggctt ctcgtccggt
     1141 gactgcatct tttcgaggct gatctcgacc gtgctccggc ccggtccgat cacgaacccg
     1201 tcgaaggttc ctccggtgta cgtctcggat gcggggtggt ccgtccggcc cgaaatccgg
     1261 acgtaaaccg aaaacggctc acggtacggg ttctccacgt cgaaccggaa ggagttccac
     1321 gggctccaat ccgctaccgg gagccgcttg atctcgaagc ccgtggttgc gtaacccttc
     1381 cagaagacgg ctttgagtat ccccgtcttc gccgaggcca gatcgcagtg cgtcgccgag
     1441 agcgcggcgg gagagaagat ctccgccagc tttctctccg cttccgcctg ccggcccgga
     1501 agcatcgctg aagaggcgca cccgagaacg aacagcgccg tcgcccgcct catttcatcg
     1561 cctcgaactc tccggtgagc cgtatgtctt ctgaagaatg gccgaccatc accttgaata
     1621 tgccgggctc tacaacgcgt ttcaggctac ggtcgagaag cgacagatcg tccggcgtca
     1681 ggaggaatcg tgctgtcttc cgctcgcccg gcttcagcgc caccttcgca aatcccttca
     1741 actgtttcac cggtgtcgag accgagctga cgacgtcgcg gatgtaaagt tgaaccacct
     1801 cggcgccctc gcgtcgtccc gtgttcttca cttcgacgct gatcggaacg tcgcggccgg
     1861 cggggacctt cggctgatca atgcgaagat tcgcgtattc aaaggctgtg tagctgagcc
     1921 cgtagccgaa ctcccaaagc ggattcggac tcatgtcgac gtatcgcttg ccccagcctt
     1981 ccttgatcca ataggcctta gacggcttga agttgtaata ggacggaagc tggcccgaat
     2041 ggcggggcac ggtgaccggg agccggccgc tcgggttgta gtcgccgaac aggacgtcgg
     2101 ccacggcgtg cccgcccttc tcgccgggca gccaggcttc gacgatcgcc ggcacgtgct
     2161 cggcgatcca gcggatggag agcggccttc cgttgaccag aacgacgacg gtcggcgtgc
     2221 ctgtctccac gacggccttg acgagatcct cctgcatgcc ggtcaggtcg aggcttgcca
     2281 cgtcgtagcc ttcaccgttg gtcggatact ccgtcgccca ggcattctcg cccaccacga
     2341 cgatggcgac atccgcgttc ttcgccgtcc gccgcgccgt ttcgatttcg ttgaccttgt
     2401 tcccaatgac gtcgcagccc ttgacatatt cgactcgcgt cccgcgcccc accttggcct
     2461 tgacgccttc gagcacggtc acgacatcct gaagcacttt gagcgcgacg tagtctccga
     2521 gcaggttcaa ctcgtggtcc gcgttcgggc cgatgaccgc gattgaccgg atgtctttct
     2581 tcagcggcaa caggttcttt tcgttcttga gcagaacgat gccttcccgg gcggcccgga
     2641 gcgacaggtc ctggctctcc ttcgtgtggg tgacgcgcac ggcccgctcg ggatcgacga
     2701 aacggctctc gaagagtccg aggtcgaact tctggcggag gatgcggcgc acggcgcggt
     2761 cgatcagcgt catcggcacc ctcccctcgc gcacgttctc cgccagcggc cgcatgtaag
     2821 cgtcctcata cgagatgccc acgtcgagtc cggccttgag cgcccacact ccagcctcct
     2881 tctggccggg agcgatgcct tcgtacacga tggtcgaaag cccgccgccc tccccaagca
     2941 ccaggccctg gaacttgagt tcgtcgcgaa gaatcttggt cagtatccag tccgaggagt
     3001 ggaccggcac agcgtcgatg gcaggatagg tcgccattac gccgcgcccg cccattttct
     3061 taatacccgc tacccacggt ggcagaaaca cctcgcgcag catgcgctcg gagatctcca
     3121 tcgcgccgcg ttcgaaaccg ctcacgggct ggctctggcc gggataatgg cagagcccgg
     3181 cgaccgtctt gtccggggcg gcgatgttgt ctccctgaac cgcgccgacg atcgtctcgg
     3241 cgatccggga acagagataa gggtcttcgc tgtagccttc ctgatttcgc ccgaggcgcg
     3301 ggtcgcggtt cggctccacg accagcgtga agatctggtg tacgccgatg gcgcgcgcct
     3361 cccgcgcagc caccgtgtag atgtcgctca gcagatgcat gttccacgtg ctgccaagag
     3421 ccggcccctc ggggaagatc gtcccaccgg agcacatcag accgtgggtc ccttcctcgg
     3481 tttgaaggag cgggatcttg aggcgtgtct tctcccgggc cagccgctgc agttcgttaa
     3541 ggaactcggc ctggcgccgg gtaccttcgg ggattacggt gttgggcagc gtaaagaatc
     3601 cgccgccagg tccgacgcct ttctcgcgcg tcccgagagt gaactggcgg catgcctcga
     3661 gcttcgccgg gatctccttg ccgagttcgt cgacatagac gcacggcatg ttcatctggc
     3721 cgatcttctc ctcgagcgtc aaccgcccga gcaggtcttc gactcttttc tcgacaggct
     3781 gttttggatc gaggtagacg ggcacgccgc cggactgggc gagtgcggtc atcgcaagta
     3841 tgaggacaag agctggaaca agatatccgt agcgcaaggt gtttctctcc cgatccatgg
     3901 tactccggcg cacaacaacc ttccgggcga gatacactga ttcctgcctt atgcgctcta
     3961 ttgccctgct ttccgttctc actttcgttc tgtgcttcgc ccaggagtcg aagcccgagc
     4021 ccgccgtcga ttggcacgac gtccggaagt tcggcgtcga gggccaggga tggaaggaca
     4081 ccaaacatcc tttcgaccgt ctccccgcca aagcggaggg agtcgtgcgc ccgcccgtct
     4141 gggatctctc gcaagactcg gcggggttgt gcgtccgctt ctcaaccgac gcctctatca
     4201 ttcgcgcccg ctggacgctg cgccacgaga accttgccct gccgcacatg ccggccaccg
     4261 gcgtcagcgg actcgacctc tatgtccggc tctcctccgg ctggcactgg atcgcgaacg
     4321 gccgcccgct gaagcagacg aacgaacaga cgctggtcgg aaactggccc ggagggaagc
     4381 gggagtatat gctctacttc ccgctttaca acggcgccga gtcggtcgaa atcggcgtac
     4441 ctcctggcgt cgccatcgaa ccgggcccac cgtatcgccc tggggtcaag cccattctct
     4501 tctatggcac ttcgattctc cagggcgggt gcgccgcccg gcccggcatg gcctacccgt
     4561 cgattatcgg ccgcatgttc gactggccga ccatcaacca gggctactcc ggaaacggca
     4621 agtccgaacc ggagatcgcc accctgttcg ccgagatcga cccggccgtc tacgtttacg
     4681 acagtctgcc gaacctcaat ccggcggaag cgaaggagcg cgttgagcct ttcctgcgga
     4741 cgctgcgcaa ggcccatccg aggactccca ttgtgctggt agagaacgtg atctacacca
     4801 acatccagtt cagtgagcag cggcggaccg aggtgacgga gaagaacgcc acgctgaagg
     4861 ccgtctatca aaagctccgc aagcaaggcg acaagaacct ctactacgtc ccggcggcag
     4921 gcctcttcgg cagcgacggc gaggctaccg tcgacggcgt ccatccgacc gatctgggct
     4981 tcctccggat ggcgcaaacg attgcacctg tgctgaaacc gctgttgcga aaacgctaag
     5041 gaacagaagg agccgaatga tgcgacctac gcgccgagaa gctgtcactg ccctggcggg
     5101 gatcactgca agctgcacgc gccgcgagcc tgccgccgaa ccgccgaaaa aggccgcccc
     5161 gcttagcctt agtgaatacg cgcccaagag catgctggta gtgcccgaga caaaggtgga
     5221 gaaggcgcgg tacccggtcg tcgacatcca cacgcacatc agttggctca ccgccgaagg
     5281 actgggccac caactggctc tgagcgggaa gcggtactac ctcgcgccgg ccgaggagat
     5341 cgtgaaggtg atggacggcc tgaacatcct cgggatggcg aacgtcacag gcggttacgg
     5401 cgcgagtttg caggagggtg tagacaacta cgaccggagg ttccccggcc gtttcttcac
     5461 cttggtcgag ccgatgttcg accaggtgaa ccagtcgggt tacgcaacca tgcagggaga
     5521 tgccgtcgaa gaggctcaca agcagggcgc gaagggcctg aagattctca agtcgctcgg
     5581 aatgtacctg cgggagaacg tcaccgaggg cccgctcatc aaagtggacg acccgcgctt
     5641 cgacccgatg tgggaggcgt gcggagcgct gaggatgccc gtcgcgatcc acgtcaccga
     5701 cccggcggca tttttctacc cggctgaccg attcaacgag cgctacgagg agatgcacca
     5761 gcaccccgac tgggactttt ccggaaagga ttaccctgcg aagatggagg tgcttgaagc
     5821 gaggaaccgc gtgctggcgc ggcacccaaa gacacaattt ctcgggctgc atatttccaa
     5881 ctggcccgaa gacctcggcc tggtgagcca gtggatggac aagtacccga acctatgggt
     5941 cgagatcggg gcgcgcatcg cggaactggg ccgccagcca cgaatggcaa ggaagttctt
     6001 cgagaagtac caggaccgga tcctgtttgg cacggacatt ttcgcacgcg gcaaaatggc
     6061 ggcggacatg gcctcgctcg ggcggatcta tccgatttac taccgcttcc tcgaaaccga
     6121 agacgagtac tttaattact cgccgggagc cgtgggaggg tcgggccgct ggcgtatcta
     6181 cggcctcggt ct
//
LOCUS       DATIFQ010000435         6003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1486142, whole genome shotgun sequence.
ACCESSION   DATIFQ010000435 DATIFQ010000000
VERSION     DATIFQ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486142"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..594
                     /locus_tag="VLE22_21795"
     CDS             265..594
                     /locus_tag="VLE22_21795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSB17099.1"
                     /translation="MAKPTDLVQGTLDLLILKTISLEPRHGWAIAKRIQQVSQEALQI
                     QQGSLYPALHRLEQQAWIKAEWRPTETGRMAKFYSLTRSGRKQLERELANWARLSTAI
                     NIVVQEA"
     gene            597..3314
                     /locus_tag="VLE22_21800"
     CDS             597..3314
                     /locus_tag="VLE22_21800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17100.1"
                     /translation="MRMLDKARLRLRSLFRRPNVEFELEAELRFHLDQLIEENISSGM
                     PPEEARRAAQRMVGGIAQYKEECRDMRRVNFVEDLIQDVRYTIRSLAKSPGFTGVVVA
                     TLALGIGANTAIFTIVHGVLLRPLDYPKPDQLMYLTAEAPAIGGTRSALSAPEYTEFR
                     QMNQSFAAVGAYSTGGAAYTTGEVNLTAGDRPLRVRSISVDAHLLKALSIQPEQGRFF
                     SDEETARWIGTLPPPIAILSHELWRTAFGGRPLVGQKVEIEGRPHEIVGIMPPGADVM
                     DNHTQVWLPLWLHPNTARQRGAHVLYVIARLKDGVTAEVAQTELSAFLANWGDRVGTT
                     DHVPTNRPLRSVDHTLQLRTLQDAIVGSSSRPIWVLQAAVGFVLLIVCANLANLVLAR
                     AGSRSREFVLRTALGASRGRLLRQAVTESAVMSGAGGILGLWLASAGVRALIRTYPTS
                     IPRTSELTIDLPVLLVALGLSTGTALLFGFVSLWRGRTSSMVTALKEGARGASGAWRH
                     YARRGLVIAQVAFAVMLVIGAGLLVQTVYNLTRIDAGFDRSRLVTFSMTLPMANSDPD
                     TRAPAYQRVLDRLRSVPGVLGTVAMSGLPPNRTPDAIATPIENYRSYDGKPFEIVDYY
                     QFVMGDYFGTMGIPIVAGRGFERTDNASQGKVAVVNETLAKRIWKGQNPIGQRLRPPG
                     GSFGATDDVWHTVIGVAKDVRQRGVEHPAGTELYVSLDQHGVSPPSMNVVLRTTLPPA
                     ALSGTIERVVREVDAAVPVVRLRDMDSVFAESIRRPRLLAQLLGAFAGLALLLAAIGT
                     YGVLSYMVTERRREIGIRVALGATRSHVLTQIMKQGLQVAALGVTIGLAGALAVNRLI
                     ASLLFGVQPTDTVTITFVIATITIVALVASWLPAWRASRLDPNVVLRDE"
     gene            complement(3791..4993)
                     /locus_tag="VLE22_21805"
     CDS             complement(3791..4993)
                     /locus_tag="VLE22_21805"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HSB17101.1"
                     /translation="MNRPPFEVADIIRTIGKSFIDRNRPWLTGLHLKVLTAIERCRTA
                     ALGGHRDRCRRCGYETTSYSYNSCRNRHCGKCQTNARNQWLAARSRELLPVPYVHVVF
                     TLPHQLARLVFHNQRILYSLLFQASAATLLEVAGDPKHLGAETGFLSVLHTWGQTLQF
                     HPHVHCVIPSGGLTPDHQRWVDPRYPFFLPLHVLSPVFRGKFVAGLKRAWRARQLCLP
                     GELQPLAQEKLFHSFVRSLYRQPWVVYAKPPFGGPHHVLHYLARYTHRVAISNHRLVN
                     FADGKVTFRWKDYAHGSKKRLMTASAEEFLRRFLLHALPRGFVRIRSFGFLSNRRRAT
                     LLPLCRRLLGAQPWHPSGEPAAPAIQPAAALWRCPQCGGPMVVIDRLSAQQLHWELAR
                     LRAIPDTS"
     gene            complement(4990..5904)
                     /locus_tag="VLE22_21810"
     CDS             complement(4990..5904)
                     /locus_tag="VLE22_21810"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HSB17102.1"
                     /translation="MTPLRRMMLEELQRRNYSKTTTRSYLQIVRDFAKHFNRPPDQLG
                     PAEIRTYQAHLLEERKLEPRTVGLHTAALRFLFVKTLKRPCPYEELPYPKRPRRLPII
                     LSQEEAARLIESASNLFHRAMLMTLYSTGMRRAELCRLKVGDIDGGRMVIHIRQGKGG
                     KDRDVPLDPKLRETLREYYRWMRPKTYLFPGTEDGWRADKPITTKVLWEACREAAQRA
                     GISKRVSPHLLRHSFATHLVENGADLPTVQALLGHADLRATSIYLHLSQRHLKAAGTP
                     LDKIEVTALDQVKRSRKLHKKLPQVEQR"
BASE COUNT         1147 a         1761 c         1932 g         1163 t
ORIGIN      
        1 cgacaagccg ggacctatgt gcccacgtgc gtccctcagc ggggtttccg ggccatatga
       61 ccgtaaccag actcttcagg ccggaccgct tggcaccggc tcaacttccg cgtggcgcat
      121 caccagttcg cagtagtaaa acgctgggcg aacaatccgg atctacccaa gatcatcttc
      181 cctgatgccg gccagttggc cagccaagag accggcttgc gcactcacac tgtgattatc
      241 tacaattgtt ggtaatctag gcatatggca aagccaaccg atcttgtcca aggcactctc
      301 gatctcttga tcctcaagac catctcgctc gagcctagac acggctgggc catcgccaag
      361 cgcatccagc aggtttcaca agaagctctc cagatccagc agggatccct gtacccggcg
      421 ctacaccgtc tggaacagca ggcctggatc aaggccgagt ggcgcccgac tgagaccggg
      481 cgaatggcaa agttctactc gcttacacgt tcgggacgga agcaactgga aagggaattg
      541 gcaaactggg ctcggctgtc gacggccatc aacatagtgg tccaggaggc ttagcaatgc
      601 ggatgctaga caaagcccgt ttgcggcttc ggtctctgtt tcgccgtccg aatgtcgagt
      661 tcgagctcga agccgaactt cgtttccacc tggatcagct cattgaagag aacatttcat
      721 ccggtatgcc gcccgaggag gcccgccggg ctgcgcagcg catggtcgga ggcatcgcgc
      781 aatacaagga ggagtgccgg gatatgcgcc gcgtaaactt tgtcgaggat cttatacaag
      841 acgtacgtta cacaattcgg tctctagcga agagtcctgg gttcacagga gttgtcgtcg
      901 ccacgctggc gcttgggatc ggagcaaaca ctgccatctt cacgatcgtg catggagtgc
      961 tgctgcggcc actcgactat ccaaaacccg atcagctgat gtatctgacg gcagaagctc
     1021 cggccattgg cggcacccga agtgcgctct ctgcgccaga gtacacggag ttccgccaga
     1081 tgaaccagtc gttcgcggcg gttggcgctt attcgaccgg cggcgcggcg tacacgaccg
     1141 gcgaggtcaa cctcacggcc ggggatcgac ctctgcgggt gcgctcgatc tccgtcgatg
     1201 cacatttgct caaggcgctc agtattcagc cggagcaggg acgcttcttc agtgacgaag
     1261 aaaccgcgcg ttggatcggg acgctaccgc caccgatcgc aattctctcg cacgaattgt
     1321 ggcgaacagc atttggagga agaccgctcg ttggacagaa ggtggaaatc gagggccgcc
     1381 cccacgaaat cgttggcatt atgccacccg gggccgacgt catggataac cacacccagg
     1441 tttggttgcc actctggctc cacccgaata cggcccggca acgcggggcc catgtcctat
     1501 acgtcattgc gcgactgaaa gacggcgtca ccgctgaggt ggcacagacg gagctgagcg
     1561 cattcctcgc aaactggggc gatcgtgtcg gcacgacaga ccatgtcccc acgaaccgtc
     1621 ccctgcgctc agtagaccac accctccaat tgcggacgtt acaggacgcg atcgttggga
     1681 gttccagccg gccgatctgg gttctgcagg ccgccgtggg attcgtgctg ctcatcgtct
     1741 gcgcgaatct tgcaaacttg gtgttggctc gcgccgggtc acggagccgt gagtttgtac
     1801 tgcgcactgc tctcggcgcc agccgcggcc ggctgctccg gcaggcggta accgaaagtg
     1861 cggtgatgtc cggagccggt ggcattttgg gtctgtggct agccagcgct ggcgtgcggg
     1921 cgctgatccg tacttacccg actagcatcc cacggacgag tgagcttacg atcgatctgc
     1981 ccgttctgct ggttgcgctc ggcctctcga ctgggactgc cctgcttttc ggattcgtgt
     2041 cgctctggcg cgggagaacg agtagcatgg tgactgcact gaaggagggc gcaaggggtg
     2101 ccagcggcgc ctggcgtcat tacgcccgtc gtggacttgt aatagcccaa gtcgctttcg
     2161 cggtgatgct ggttatcgga gcgggtttgc tagttcagac agtctacaac ctcacgagga
     2221 tcgatgctgg atttgaccgg tcgcggctgg tgacgttctc gatgacattg ccaatggcga
     2281 attcagatcc cgacacacgc gcgccggcat atcagcgcgt cctcgacagg cttcgctccg
     2341 taccgggcgt gctgggaacg gttgccatgt cgggtctgcc gccgaatcga actccagatg
     2401 cgatcgctac tcctatcgag aactacagat cttacgatgg aaagcccttt gaaattgtcg
     2461 actactacca attcgtcatg ggcgactatt tcgggacgat gggcatcccg atcgtcgccg
     2521 gccgcggctt cgagcgaacc gacaacgcat cgcaaggcaa ggtggccgtc gtcaatgaga
     2581 ctttggcgaa aaggatctgg aaggggcaga acccgattgg ccagcgtctg cggcctccgg
     2641 gtggttcgtt tggcgctacc gatgatgtct ggcatacggt gatcggagta gccaaggacg
     2701 tcaggcagcg cggggtggag cacccagcag gaactgaact ttacgtatcc ctcgaccagc
     2761 atggcgtctc accaccgagt atgaatgtgg tgctgcggac gacgctgcca ccggccgcgc
     2821 tgtcagggac gatcgagcgg gtggttcggg aagtggacgc cgcggttccc gttgtgcggc
     2881 ttcgcgacat ggattcggtg ttcgccgaat cgattcgccg accgagactc ctcgcgcagc
     2941 tattgggtgc atttgccgga ctggcgctgc tactggcagc tatcggtact tacggtgtgc
     3001 tctcctacat ggtgacggag cgtcggcgcg agatcggcat tcgggtcgcc cttggtgcaa
     3061 ctcgctccca cgtgctcaca cagatcatga agcaaggcct tcaagtggcg gcgttgggtg
     3121 tcaccattgg ccttgcaggc gcgcttgccg taaatcggct gattgcgtcc ctgctctttg
     3181 gtgtgcagcc gacagatacc gtgacgatca cctttgtgat cgcaacgatt acgatcgtgg
     3241 cgctggtcgc aagttggctg ccggcgtggc gggcatcgcg gctagacccg aacgtcgtgc
     3301 tcagagatga gtaacagctg agaacgctct tacgccatac tgttgttttc ctgggcggat
     3361 cgccgctaca cggaagttga ggcctgtgaa acggcgttaa ccagaaggtg ccaggaaagc
     3421 cagatgccga ttacccgccg ctgacgctat gactctatct ggagtgaccc ggtgacgtac
     3481 tgcgcgggcg cgatggaatg ccggctgccg ggtcacttcg gatagggacg tttgaaggaa
     3541 gccgctcgcg tttgcggcgg gcgcggccgg ttatggattt tgaatcggat ttcgatgagc
     3601 cggttgccca aatcgaccgg ggaggctggc gcaacagagg cgcatcccgc gaggtggacc
     3661 ggctggcaag ggcggtcggc cccgttttgc gggcgaggtg agggggctgc ggggggtcga
     3721 agacggatca agacacacat caaacgcgcg tgccggagca caccaggagg tcgccgtggt
     3781 tggccgtcga ctaggacgta tcaggaatgg ctctcagcct tgccagctcc cagtggagct
     3841 gctgggcgga aagccgatcg atcaccacca tcgggccacc acattgtgga caccgccaga
     3901 gtgcggcggc gggctgaatg gctggagcgg ccggttcccc agacgggtgc cacggctgcg
     3961 cccccaacag acgccggcac agcggcagca gcgtcgcacg ccggcgattc gacaggaatc
     4021 cgaacgaacg aatgcggacg aagccgcggg gcagagcgtg gagcaggaag cgccgcaaga
     4081 actcctcggc gctggccgtc atcaagcgct tcttgctgcc atgggcgtag tccttccagc
     4141 ggaaggtcac tttgccgtcg gcgaagttga ccagccggtg gttggagatg gccacgcgat
     4201 gggtgtagcg agccagatag tggagaacgt ggtgcggccc gccgaatggc ggtttggcgt
     4261 agacgaccca gggctggcgg tacagtgacc gaacaaagga gtggaacaac ttctcctgcg
     4321 ccagcggttg cagctcgccg ggcagacaga gttgccgggc gcgccaggcc cgcttcaggc
     4381 cggcgacgaa cttgccgcgg aacactgggc tgagaacgtg cagcggcaag aagaacggat
     4441 agcgcgggtc aacccaccgt tggtgatccg gggtcagccc gccggatgga attacgcagt
     4501 gaacgtgcgg gtgaaactgc agcgtctgac cccacgtgtg gagcacgctc aagaaaccag
     4561 tctcggcccc caaatgcttg gggtcgccgg cgacttccag cagcgtggcg gcgctggctt
     4621 ggaacagcag actgtagagg atccgctgat tatggaagac caggcgagcc agttggtgcg
     4681 gcaaggtgaa gaccacatgc acgtagggca ccggcagcag ttcacggctg cgcgcggcta
     4741 accattggtt gcgtgcattg gtctggcact tgccacagtg gcggttgcgg caggagttgt
     4801 acgaatacga ggtggtctcg tagccacagc gacggcaccg gtcccggtgc ccaccgagcg
     4861 ccgccgttcg gcagcgctcg atggccgtca acaccttcag gtgcagaccg gtcaaccagg
     4921 gtcgattgcg atcgatgaag ctcttgccta tggtgcgaat gatatcggcc acctcgaacg
     4981 gtggccggtt catctctgct ccacctgagg cagcttcttg tgaagcttgc gggaccgctt
     5041 cacttggtcc agggccgtca cctcgatctt gtcgagcgga gtgcccgcgg ccttcagatg
     5101 ccgctgcgac agatgcaggt agatggaggt ggcccgaaga tcggcgtgtc caaggagagc
     5161 ctgtacggtc ggcagatcgg cgccgttctc gaccagatgc gtagcgaagc tgtgacgaag
     5221 caggtgcggg ctcacgcgtt tgctgatgcc cgcacgttgc gcggcttccc ggcaggcttc
     5281 ccacagcact ttggtggtga tgggcttgtc ggcacgccag ccgtcctcgg tgccggggaa
     5341 caagtacgtc ttgggccgca tccaccggta gtactcacgg agcgtttcgc ggagcttggg
     5401 atccaacggc acatcccgat ccttcccgcc tttgccctgc cggatatgga tgaccatgcg
     5461 gccgccgtca atatctccta ctttcagccg gcacaactcg gcgcgccgca tcccggttga
     5521 atacagcgtc atgagcatgg ctcgatggaa caggttgctg gccgattcga tcagtcgggc
     5581 tgcctcctcc tgactgagaa tgatcggcag ccgccgaggg cgcttcggat acgggagctc
     5641 ctcgtacggg cagggtcgct tcagcgtctt gacgaacagg aagcgcaagg cggccgtgtg
     5701 caaacccacg gtgcgcggct ccagcttgcg ctcctcgagt agatgcgcct ggtaggtgcg
     5761 gatctcggcg ggtccgagtt gatcgggcgg ccggttgaag tgcttggcga aatcgcggac
     5821 gatctgcaga tacgaacgcg ttgtagtctt agagtagtta cgacgctgga gttcctccag
     5881 catcattctt cgcaatgggg tcacaaggac ctcctttcct tgtgacccaa actattccac
     5941 tcaacacggg aatgctgctc tctttacgca cgcgtagcgt ccgccgcgcg agcggcttcg
     6001 ttc
//
LOCUS       DATIFQ010000436        31969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2443868, whole genome shotgun sequence.
ACCESSION   DATIFQ010000436 DATIFQ010000000
VERSION     DATIFQ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 31969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31969
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443868"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..938)
                     /locus_tag="VLE22_21815"
     CDS             complement(153..938)
                     /locus_tag="VLE22_21815"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HSB17103.1"
                     /translation="MMNRRFFLEAMGGMAMLTPGLAAQATGERKTRFYLLETYHLKHG
                     TQLGRLHDYLSQSALPAFGKLHSGPKIVLEALVAAHMPQVAVIIGFRSLAEMWDIRAR
                     LNRDEELKKAFEAWQAGPEPPFELQNNVLLEATGYSPEIAPLDPPPSTPRIFELRVYH
                     SPTWRQLQALHERFEGPEIKIFHRVGIRPLLYSSTVIGPNMPNLTYLIPFDDLAAREK
                     AWNAFGADEEWIKVRKESIDKDGQIANVIQMALYRATTYSPIR"
     gene            989..3670
                     /locus_tag="VLE22_21820"
     CDS             989..3670
                     /locus_tag="VLE22_21820"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSB17104.1"
                     /translation="MRLAIILAAVLTSVAADTTLDHRAPAGTRTAMRRPGAHTILPGG
                     KIIEPLGLQYHTGPGPFGLAVNPKGSIVVSADGGPQRYSITVLEKRKERWRVRQYETE
                     RSAEHQNDEDWRSVFMGLAFQSERELFASEGNSGRVRLLDPLKGKARWVIDLNQGEYS
                     DSYTGDLAYDEGRHLLYVVDQANFRIVIVDTRRRQVISSVRVGRLPFAIALAPGGKRA
                     YVTNLGMFQYQPVPGADPKRARETGLPFPAFGFPSPEALHGVKRETSLGSVDVPGLGD
                     PNVPESNSLCIVNVEDPAKPRIETFVRTGLPFGGKSSGGSSPSGVLAAAGRIFVSNAH
                     NDTISIVDPATNSVAGEIPIRIPGLESLRGVLPLGMAYHEAASWLLVAEAGINAVGVI
                     DTREMRFLGHLPACWFPTRVRIDRDNVYVANAKGHGTGPNAGPRSALRQSFQALLRRG
                     TISIYPLPGRDELERQTSRVLRLNGFVPIKQARAKLPQAVRHLVLIVKENRTFDEMLG
                     DVRTASNGEVRGAPALARFGRFGWVQQDRRELRTRLALRDIDVAPNHRAIATRWAFSD
                     NFYADSEVSVDGHHWLVGSYPNAWTESSLMAAYGGEKDFRMPTAAPGRLQFPQSNSSV
                     HPEEQLEAGAIWHHLERHGISFRNYGEGFELAGVDEGEGLKPTGARYLTNVPMPDPLY
                     RNTSREYPQFNMNIPDQYRASQFIAEVERRYLKGGEPFPRFIFIHLPNDHMARPRPGD
                     GYPFNASYVADNDLALGRIVEFLSRSPWWREMAIFVTEDDAQGGVDHIDSHRTVLLVA
                     SPWAKRNYVSRENSSFPGLLKTVFRLLDVPPLNLFDGAAADLSDCFTSEADFTPYEVR
                     PVDPRLFDPATAREPLDPTPSPSMDDPRFLQQQHSNR"
     gene            3763..4464
                     /locus_tag="VLE22_21825"
     CDS             3763..4464
                     /locus_tag="VLE22_21825"
                     /inference="COORDINATES: protein motif:HMM:NF006047.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HSB17105.1"
                     /translation="MMLEDLRGEVLKANLELVRRKLVIYTFGNASGISREHGLVAIKP
                     SGVPFEDLKPEDMVLTDLNGKVVDGKLRPSSDLATHLLLYRTFEDIGGVVHTHSSYAT
                     AWAQAEQEIPCFGTTHADYFSGAVPVTRALRPEEIEKDYEENTGLAIVRRLRDIHPLD
                     MPAVLVAGHGPFCWGPSATKAAQTAVILEEVAKIAYRTAMLEPMAWPISGALLKKHFH
                     RKHGPTAYYGQERKS"
     gene            4461..6476
                     /locus_tag="VLE22_21830"
     CDS             4461..6476
                     /locus_tag="VLE22_21830"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSB17106.1"
                     /translation="MNGIHGIKLCAAAVAVLAGAVDARCATYYVDCAGGSDTNIGTSV
                     TAAWRTPAKVSGITFAPGDSILLRRGTECRGQLWPKGSGAQDHPITIGAYGSGPLPII
                     RGEADAPVVKLHNQQHWHIENIESSGGSPYGIFISGDRGVLRHFRIRDVVVHDVGGTA
                     KTKDSGLIAVVAGGKDQTFDDVVIDGVTAYNTTQWAGIVVNGAAFTRPQGSTVRARNA
                     TIRNSVVHDVYGDGIILFQVSNGLIEKCVTWNTGMEPTQTIGTPNAIWTWKCNTCTVQ
                     FNEGFFADSPGVDGGVYDIDWGNENNIIQYNYAHDSQGYCASVFGAGNLVTTNSVVRY
                     NLCVNNGRSPRLAKRQGEIFLSTWDGGKLDGVRIYGNTIYWNPPVEAPVVFDQADYTG
                     KKPNFFKNNLIYSTVRSLVKSTSSMKLDHNLYWYGGTGEPEWLFGGTLHRGFARFGQA
                     TGQEAHGSFAEPKLVLPELTLAADSPAVNAGDDVGNMGKHDLSGFPIPQGGAFDIGAL
                     EYTGPMATGSSGPAPAFALKDTAGFAHTLGQFRGEWLLLSFLEQSDGDASASRTQAVF
                     LKSVLYQHGNQGLKVAVVARAGVAGTNVVNQAHDWRLRDIPLLLDESGSVERGYGVTT
                     KPSTFLIAPAGQIVRRWNGFASAAQLGLTLRWLIGPPPGTPPVSLAR"
     gene            complement(6483..8072)
                     /locus_tag="VLE22_21835"
     CDS             complement(6483..8072)
                     /locus_tag="VLE22_21835"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HSB17107.1"
                     /translation="MAIVAGVDFGTLSVRVSIVDSEKGRIGSATADYPLFRKKEDPDH
                     ATQRHQDHMDGLAAAMKKAIEAAAVSGRDVEAIALDTTGSSVVPVGDDLVPLDDYYLW
                     CDHRAKGEAALITNTARREKLAAIDWCGGVYSSEWGFSKLLHWLRNNPEKRGRLATAL
                     EHCDMVAAVLCGINDPRQVPRSICAMGHKWMWNEELGGLPPEEFLVTVDPLLAGVRGR
                     IDGCYATSDQIAGRLTPEWAARLGLKSGIPIPVGAFDAHWDAIGAGIREGDVVNVVGT
                     STCIMAIAKHVDLIPGVCGVVKGSIHPQCWGIEAGLSATGDIFDAIARRAGKNVAELS
                     RGLDQYRAGQTGLLRLTWDNGDRTVLVNAELGGVTLGWNLTHTAQDELFAAIEGTAFH
                     TRVILERMAEHGVPINRVINGGGVPQKNETLNRIYANVLNKPVLVPQDEVTSLGSAIF
                     AFLAAGVFRTVEEAQDALCPKYRTVEPVAAEAAVCERLYSLYRKLYFGFGRPGSDAIP
                     IGDVLPELRVIAAQVRSNNAG"
     gene            complement(8137..9549)
                     /locus_tag="VLE22_21840"
     CDS             complement(8137..9549)
                     /locus_tag="VLE22_21840"
                     /inference="COORDINATES: protein motif:HMM:NF014948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HSB17108.1"
                     /translation="MRRQMTFGLIIGNRGFFPDHLAKSGREEMIRAVQGAGMSVVVLG
                     PDDSKYGAVETRAEARKCADLFRKHRDSIDGVIVSLPNFGDERGVAETLRMAGLNVPV
                     LVQATPDTAGKMLIADRRDSFCGKMSVCNNLTQFGIPYSLTTLHTERPDSEVFRRDLE
                     WFAAVCRVVNGLRKARVGAIGARPAAFNTVRYSEKLLEACGIDVDPLDLSEVLGRVEK
                     MKDDDPAAQEKLQAIKSYVSTKDVPSESLLKMAKLGAVIDGWMQSNDITVSAVQCWTS
                     IEEYFGVVPCTVMSMMSDRLMPSACEVDVCGTVAMYAMALASETPSALLDWNNNYGDD
                     PNKAVCFHCSNLPKHFFADVRMDYQEIIAGSVGKLNTFGTCVGRVKSGPMTYFRVSTN
                     DRCGRIACYTGEGRFTDDPLETFGGAGVVEIPRMQDLLRYICENGFEHHVAANFSSTA
                     SVVHEAAKRYMGWEVYWHKA"
     gene            complement(9593..11701)
                     /locus_tag="VLE22_21845"
     CDS             complement(9593..11701)
                     /locus_tag="VLE22_21845"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HSB17109.1"
                     /translation="MPRLLLLLYLVPLLAQAQTPKIDTILYGASYYHEYMHYERLEQD
                     VQLMEKAGLTVMRVGESTWSSWEPRDGEFEFAWMDRIIDRLHKAGIKVILGTPTYSIP
                     PWLFQKHPEILVSQLTKAPPLTHPTQATYPGAAVPGAYGPRQNMDITHPTYRWYAERV
                     IRAVVSHFKDHPAVIGYQIDNETHPNSTNSLSMHTDFVTYLKRKFGTTDALNKLWGFA
                     YWGQLVNGWDEFPPREGILNPGYKLEWERYQQKTVTDFLAWQAAIVKEYKRPGQFITH
                     DFVGGVRADVNQFDIAEHLDITAVNPYHAVQDELDGLAISLSGDVCRSLKQANYLVTE
                     TNAQTIGWDSRTQYPPYDGQLRLSVYSHVASGANMVAYWHWHSLHYGQETYWKGILSH
                     DLEPNRAYTEVTRIAQELKKLGPQLANLKKKSDVAILYSIDSHHGIQFMPFHDRVNYM
                     AVLQQMYGALYRLNVQTDFVFPQSKKLANYKIILVPPLYVASDELLTRLADYVKDGGH
                     LVMAFKSGFTNEHSTVRWVMAPGPLRQAAGFHYQEFSNLAEPLALKGDPYGAGERNRV
                     SAWAEFLIPETATPLATYDHPFFGSFPALTRNKHGKGTLTYEGTYLSDELQEKLLAEV
                     MGLAGLLGSDQKLPAAVRVKHGTGNRGKRMHYFLNYSGQPQTFSYSYAGGTDLLTGRA
                     VNGAQSVTLPPWDVAIIEER"
     gene            complement(11787..15392)
                     /locus_tag="VLE22_21850"
     CDS             complement(11787..15392)
                     /locus_tag="VLE22_21850"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB17110.1"
                     /translation="MKRLTILLLLAALPPFIGALSAQSDRGAITGTVTDATGAVVPGV
                     QIAATNLSTGVVFTATTNEVGLYSVLNIPIGRYSVAFTKQGFKTYTRDGISIAVAQVV
                     QLNANMEVGALAEAVTVTAEAQLLETQSTQVGAGMNSNLVTDLPLDIRGGRSLENFAY
                     ATIPGVEGNNWTSYIGGSMAFTKEVLIDGTSAVVQIGGHIGESSPSMEAIQEFKVETS
                     GIRAEDGRTGGGVFKFTLKSGTNDFHGSAFGFLHNEVLNANTWQSNFQGDKRATDRQF
                     VYGFSAGGPIIRNKTFAFGAFEKYTQERFVLGAYNATVPLPAFLDGNFSELLNTGSVL
                     GQDALGRPVYQGMIYDPATQRNVTAGSVDPVTGLTAARTGMVREGFGFDPVTGAPIPS
                     AANVIPSNRISTISKNITDVFRQRYLPMRPGLINNTAITRQNDPWFHQTQLTFKADHN
                     FSDANRLSGHFVWTERPRILVDQGGIWDPADPDKAGGPMANSRKQEVTSRRFALNHTY
                     NFSPTVLNIASFTFGRYRNPSFSVAAKDDWPSKLGFGETGAGNFPVIEFGRAINGIDT
                     TRIGQHWSDYYVGNTFIGNESLTWIRGRHTMRFGGEFRAFQLSSHQSTPTLEFQFDPD
                     QTGAPKEAWRDKVGFGFASFLLGGPKEASQQTPWDLYGRRKAFSIFAQDDFKISRNLT
                     LTMDLRWEVTFPYHEKYGRWANFDKKAISESFGIPGTLVFAKNGDDTFETERDWKEFS
                     PHVGIAYQIGSRAVLRAGYGLFYSPIGMNYWFGVPYGFAPGYRGTNRVTPTADLSPAF
                     NWDVGYPGTFVPGTKDPDYTQWGMVNVNPKSLFAGYSHQWNIGTQFTLTNDLRLDVTY
                     MQNEGRRLQSGDFERNQPDPGAMANLIRSGHVWDAVSDAGSAASAGVPFPFEGFGSYA
                     FAAIMPYPQIWQNWVWGGPLLHVGSPYGRSDYKALQLAVTKRTERGLTANVSYTLATS
                     HSNLQNSLGGFQEIWSSGPLQDVLHLDEEAKAPLFYDQRHVFKGIVAWDLPFGTGRRY
                     LNGAGRVANAILGGWTLSGSFRYNSGSPIGVNSSNYYPGWEAPVYALRNENGDYSRNF
                     EGSDFNFGDPGLPGNLYFDPKNFSNPPDGDLGTVPMFRSDFRGFGWSEEQVAILKNFR
                     IAERFRLQFRAEFYNVFNRHHFDTPDTNISSGTFGHVINLTGTPREGQFGARFEW"
     gene            15678..16673
                     /locus_tag="VLE22_21855"
     CDS             15678..16673
                     /locus_tag="VLE22_21855"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSB17111.1"
                     /translation="MTLEEVARRAGVSTATVSRVLNKAGVVRETTRKRVLQAARELRY
                     HPNLHARTLAGAKSNTLGMIVSNIENPFFIDIFRSLESAALQRGYEVVVEHTDYKSSR
                     LVTCARAMLGRRVAGLAAIVSEMEPSLIEELTESDVPVVFYDVGLPARNITNIRVRYE
                     RGMQRTVEYLYSMGHRRMAFVGHHASLGPLQARKKTFIETMARYSGQVQFTAALNTDS
                     PAGGKQAAHELLASDFRPTAVLCVNDFMALGVIRELRDQGFSVPRDISVTGFDNIQLS
                     EFSNPALTTVNIPRRKIGQMAFEALAPKESSGDRPREFVIDPELLVRESTGPPPV"
     gene            16694..17692
                     /locus_tag="VLE22_21860"
     CDS             16694..17692
                     /locus_tag="VLE22_21860"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB17112.1"
                     /translation="MKVLKPLFISLLLVLPVAAQLPDFYRTVDRLIWVVDDLDRTTQG
                     WQKLGFAGIEYQGEAELPALFRGKAGTATLRVASGRIGDVPVHWVQPISGKDAFSEFL
                     ARNGSGVFSIMHRVPSREAFDAEVKRMADIGVGVLQGGEAETEAGPVRFAFLDTAARG
                     KYVLGLLHVPGAADLALAPPGNPSGRKVAQYAFVAKDLDAVSAYWARLGFPAMTYTHP
                     DSSELRYRERPGEFDMKLGWQRHGKVTYEWILSLKGPNVYEDHMKKHGEGLHHLAFEV
                     EDIDKEAADWQAKGFPFGMGGAWGQKGKPGSGRFIYMDTHAIGGIDIELLWSFRAK"
     gene            17856..18464
                     /locus_tag="VLE22_21865"
     CDS             17856..18464
                     /locus_tag="VLE22_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17113.1"
                     /translation="MSFQGWLALILAAALVAPPVLSAQEAAPPPPKQSGGTLKIVTLE
                     GEGAKNVIKTRYAVQPVVEVHDQNDKPVSGAEVVFQLPAAGPGGVFNGWMRTQTLRTD
                     AEGKAGTNGFTPNDEEGRFNIKVTATSNGKTGSAIIAQSNTRTGGQSSSGASKWWKVL
                     AIAGGAAAAGGIVAATRNGDSSGAGNNPVTIAPGTITVTGPR"
     gene            18500..19480
                     /locus_tag="VLE22_21870"
     CDS             18500..19480
                     /locus_tag="VLE22_21870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17114.1"
                     /translation="MRNLKFFPCGLLLLGLGAAFGQDGGLTGPVSGMVFDADSRSLRP
                     VVGVPGAAYLGQPAVSGLDWASVAPNGRAALVLGNAQLRLLRWSSDGTSRADIIVEEA
                     QTPGRVAWSADSSAVAVYENSGRLRVWRTQTAQPAGDIDLSLLSGRISALAVEKSGAV
                     VIGVEDATAGGVFRVSPGAAPVMVAAMASPGAMAFSRNGRDLFVADRATRRVIEIRGY
                     RASAALIPLADETSGVVEPAALAVSEDGMRLFLADRGKNTVGICDLTTRTLAGEIALD
                     FAPVSLEPLSGQSLYLLKGGGGGEPFQVLSAGREPAVYFVPAERLVLEER"
     gene            19483..21669
                     /locus_tag="VLE22_21875"
     CDS             19483..21669
                     /locus_tag="VLE22_21875"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HSB17115.1"
                     /translation="MSRTTLVLVAAVLISFLLAPAALAQSVPFTLRYDQGDTTTVVSN
                     NSSVFLPADAVGQAAVATLTVTYRGTTSVSITEVEMLDAFEFPSADFSVSGLPSLPLS
                     LTENQSFSVTLRYQAATRNRVIGRVLFHYKQALVTSQITLNLVGVAPEFSYSYIPQGG
                     NPAVLAPGGTIAFLTTAVDGASTATVVITNKGSGAGKVDAISSSGAAFELSGLPLPPA
                     TITAARELRFTVTYKPTALETSSGQLTLDLAGGRVTFALQGVSEGPAFVYEKIESSAV
                     VPVIPGQVVSLPDTLVGEETSVPFRVRNDGNHDGQIASISVLGDGYQVTDLPFLPLVL
                     KPGASATFTVKFSPSQPGKISGRLRVGDDSFDLAASGLGAALEYSLVVGSTTTTVPSG
                     GIITLPQVAVGESVKARFIVTNTGTAEASINSITLPAASPAVFLLEELPSLPAKVAPQ
                     ATTGFTVVFKPSVVGNASSTLKVDNVTFTISGAGSSPAPLPAYTFEGASGVQEPLQQP
                     GVGLTLSEPYSLGLTGTLRLAFTSEAFSSDPSVQFSSGGRTVNFTIPANSTRAVFPNG
                     TNEIRLQTGTVAGTITLTPSFATEGGIDMTPDVPAVLNLMVPQSAPRVLGVQITSKTT
                     AGFTVVVTGFATGRSLTQMDFQFNPVSGENVSTTKVSVNVEASFLSWYQSAASQQYGS
                     QFLVSIPFTMQGDVRNVNSLIDTIQSVAVTIGNRQGTSSAVTANLR"
     gene            21774..23264
                     /locus_tag="VLE22_21880"
     CDS             21774..23264
                     /locus_tag="VLE22_21880"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HSB17116.1"
                     /translation="MSASEMTRRAFAGAVAAAGFCFAQDGWIDLFDGQSLRGWRPSEN
                     KNSWKVVGGQLLADGPRSHLFYDGPVREAGFKNFELEVEALAHPAANSGIYFHTAYQE
                     TGYPRKGFEVQINNTATGEGGYRERKKTGSLYGVRNTYKQLVADDGWFKIHVAVRGKN
                     IQVRLNGILLVDFTEFTPPFIAAGNERERFLDRGTFALQGHDPGSKVRFRRIRVRPLP
                     DDVPTPGSASAAADALEQDVITLGARNIPLVDYHVHLKASLTLDQALAKSRRDGIQHG
                     IAINCGQGFPVQNDDSARRFVDEMKGQPVFLAMQAEGREWTRMFSRRAAALFDYVFTD
                     SMTWTDNRGRRMRLWIPEEVGTIPNPREFMDTLVERTIGILENEPVDIYVNPTFLPKP
                     LDGNYDTLWTDEHMRKVARAAARNSVAIEINDRFKLPSAAFLRVAKEEGCKFALGTNN
                     GGPTDLGRCEYGVRMTTELKLSWQDFFVPGAWGPKAVERKPGALRA"
     gene            complement(23282..24103)
                     /locus_tag="VLE22_21885"
     CDS             complement(23282..24103)
                     /locus_tag="VLE22_21885"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSB17117.1"
                     /translation="MSNPLRLMCVLAHPDDESLGMGGTLARYAGEGIETYLVTATRGE
                     RGRIGPNGEREAPDVAGRIREEELLAAAGELGVREVRFLDYLDGDLDQADPSEAIGKI
                     AGHLRRVRPQVVVTFGPEGAYGHPDHIAICQFTTAAVVCAADPEYKGAEDGPGRAVSH
                     RVSKLYYMAWTQAKWAAYQAAFRNLKVTVDGADRVVAPWPNWAVTTVIDTSAFWSKVW
                     RAVSCHKSQMAIYGKLEHLSDEHHRALWGSQEYYRVFSSVNGGRMRESDLFDGLR"
     gene            complement(24193..24268)
                     /locus_tag="VLE22_21890"
     tRNA            complement(24193..24268)
                     /locus_tag="VLE22_21890"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24233..24235),aa:Gly,seq:gcc)
     gene            24380..26485
                     /locus_tag="VLE22_21895"
     CDS             24380..26485
                     /locus_tag="VLE22_21895"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HSB17118.1"
                     /translation="MLTHSFRSATLLPVLGLLLFSGCTRRPASQPASETPLPRPGWLK
                     KEPLIVAGNWDSMAIFRRRVGGNPVWQEEDYAREHTEETVRKLKDLGVTMAVIHFYKG
                     YGMRAESEQLEDSRKLAALCKKYGIRVGVYVGSTVAYETFLAEAPDGIDWFVPPYLGR
                     PVFYPGQSFRKRVYFMHPGYREYMKRVLDVAIKDLKVDLIHFDNTSLQAQAPVFYHSQ
                     AVSDFRDFLSAKYTPEMLKKRLGFSEVRFIEPPIVDRVVSVLNDPLVQEWADFRCRQL
                     AAYYGEMERYIRGLNPEVAVENNPHSGVSGINTVWEQGVDYPRLLAHTDVVWSEEGNE
                     AGVTPEGVLLSKIRTFKMASALNNSIFTYTGGDQGSTLQMAEAMVYNRRNLGMVGGVL
                     AGYDTPADQRNYIRFYRDNFDLYRDAASRSDVAVLHSYASLAYNNDLPWQSTMLFEQA
                     LIQSKVPFDIIFDDHLKDLSKYRVLVLADQECLTDEQLEQVRRFVAAGGGLVATEHTS
                     LYTGWRQRRRDFGLRDLFGISFSVSPAAWPGSRAAEPVMKIAAVQRQADKGRVAYIPS
                     VEPAVPKPDGAPMRSEYWKLPLNMKELIDAVHWAGGGLLSIEVKAPPTVTAEVLEDTT
                     HNRLTVHLINFDCERAPKLSGLTVNLAIPAGRTVAKVTWLSPDEKGPLPLTSSLRNGR
                     AALLIPSLETYGVAVLELH"
     gene            26815..28137
                     /gene="dnaA"
                     /locus_tag="VLE22_21900"
     CDS             26815..28137
                     /gene="dnaA"
                     /locus_tag="VLE22_21900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HSB17119.1"
                     /translation="MNEWEQIKKHIAGRLSNESYQNWLAKTSFHQLDGEVLRVSVPND
                     ATRAWLEEEYSDLVSASVHELNLRIHRVAYELPGATIPDLRANNGHDGVVPELMFRSP
                     DSHLNPRLTFDTFVVGSCNQFAHAAAKAVAAMPSRNYNPLFIYGGVGMGKTHLMHAIG
                     RSLLDNFSGMKIVYTSSERFMNEMIQCIATQRMSHFHQHYRSADILLVDDIHILGGKE
                     RTQDEFFHTFNELYDHQKQIVISSDSSPKDIPGLVERLRSRFEWGLMVDIQPPDLETK
                     MAILDKKAEAEGITLPEDVRIFIATKTKSNVRELEGALVKLIAYSSMSGTPINLPMAQ
                     NCLRHLVHGQERRITIESIVRSVAERFSLQPSQLKQKSNARAIAYPRQVAMYLVKELT
                     SASLPEIGRAFGGKHHTTVLHSVQKIETQRHQDPDLNRMLHSLIDSLS"
     gene            28380..29495
                     /gene="dnaN"
                     /locus_tag="VLE22_21905"
     CDS             28380..29495
                     /gene="dnaN"
                     /locus_tag="VLE22_21905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HSB17120.1"
                     /translation="MDFIVSKNDLVRELNLSQGVVEKKTTIPILSNVLIEASGDKVNL
                     TATDLELGIRCSCPARVNKDGAGTIPARRLLDYVRLLPEAELHVKFMENQWANLVCGR
                     SRTRIAGMSRESFPELPEFPEVLAEIPVSELSGLIAKTIFAISSEESRFTLSGALLLL
                     KSQGMSMVSTDGHRLAFVDTKSELAGVKGSYRALLPKKAMGEILKLAQESAPDASVQF
                     AGDENHLFFRFGERLLISRKLTGNFPDFERVLPREQPLSVILGREEARAAIERVAQFS
                     DERSHAVRIQVCPGEVKVHSSISETGESEESIPTEYDGATVEIGFNAQYMLDFLRASE
                     EDKVVLHFKDGHSAGELRPADDAGKYDYRYVVMPMRI"
     gene            29580..>31969
                     /gene="gyrB"
                     /locus_tag="VLE22_21910"
     CDS             29580..>31969
                     /gene="gyrB"
                     /locus_tag="VLE22_21910"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HSB17121.1"
                     /translation="MGSKDVELNTVDTYDSASIKVLEGLEAVRLRPAMYIGSTGETGL
                     HHLVYEVVDNSVDEALAGFCDEINVTIHFDNSVTVVDNGRGIPVDIHVEEGISAATVV
                     MTKLHAGGKFDSKSYKVSGGLHGVGVSCVNALSERLELEIWRQGFTWTQEFERGNPVA
                     PLQRAGKAGRKTGTKVTFKPDPTIMVVTEFNFDILSQRLREIAFLNKGLRITMTDERV
                     DPPKSHEFQYNGGIAEFIKHLNRGKTVLHDKPIAFEGERELPNGGTLTMDVALQYNDS
                     YAENLFSFANNINTVDGGSHLTGFRSALTRTINSYGQQAGLFKDVKENLTGDDVREGL
                     TAVVSVKLPQPQFEGQTKGKLNSDIQGFVVQFVNEKLSEYFDKNPSVMRKIVGKAIEA
                     SRAREAARKARDLTRRKGALDSGGLPGKLADCQERDPERCELFLVEGESAGGTAKAGR
                     DRRYQAILPLKGKILNVEKARYDKMLGHDEIRAMITAIGTGIGKEEGDFDLAKLRYGK
                     IIIMTDADVDGSHIRTLLLTFFFRHMQDLINRGHVFVAQPPLYRIKKGKSERYIKDEK
                     EFMREILRRATENVVVEVSAHNGSPSQRIEGTELRNFLMALEEYLQISSKAERKLRDS
                     RAVTVVSNPELKIDSKSDFGDENNLKPLARALEAAGIPAELKHDEEHSSWMVSFRDST
                     HAERTIGVELAAMPEYRRLRSLGRQIGRYNQPPFVVLKDSSRVIQEHWRGLLDYVKEE
                     GKKDASVQRYKGLGEMNAEQLWETTMNAEKRTLLRVRLEDAVECEEIFSTLMGEDV"
BASE COUNT         6203 a        10008 c         9453 g         6305 t
ORIGIN      
        1 gtcgaaatgg tgatgcgaag agaccttagt ctgcccatca aggaagcggt tttcaagctc
       61 ggttttgtct tcctgttggg aataatggtc ttcgtgattt acaacgacat cgtcaaggtg
      121 ttgccgccgg gctgacgcac ctacacagcc gttcagcgaa tgggtgagta cgtagtcgct
      181 ctgtagagcg ccatctggat cacgttggcg atctgcccgt ccttgtcgat ggattccttc
      241 ctgaccttga tccactcttc atcggcgccg aaagcgttcc acgctttctc acgggcggca
      301 aggtcgtcga acggaatcag gtaggtcaga ttgggcatgt tgggcccgat caccgtggag
      361 ctgtacagta gcggtcgaat tccaacccga tggaagatct tgatttcggg tccctcaaac
      421 cgttcgtgga gcgcctgaag ctgccgccac gtaggggaat ggtacacgcg aagctcgaag
      481 attcgcggcg tgctgggcgg tggatcgagg ggcgcgattt ccggtgaata accggtggcc
      541 tccaacagca cgttattctg gagttcgaac ggtggttctg gacccgcctg ccaggcttcg
      601 aaggccttct tcaactcctc atcccggttg agtctcgccc gaatgtccca catctcggcc
      661 agagaccgaa atccgataat aacggccacc tgcggcatgt gtgccgcgac gagtgcttcc
      721 agcacgatct ttggaccact gtgcaacttt ccaaacgcgg gcagagccga ctggctgaga
      781 taatcgtgca atcgccccaa ttgcgttccg tgcttcagat gatatgtctc gaggagatag
      841 aaacgtgtct tccgttcgcc ggtagcctgc gccgccaagc cgggtgtgag catcgccatt
      901 cctcccatcg cttctagaaa gaaccttcgg ttcatcatcg ccttgattgc ctggtactcc
      961 atccctaaga tataagcgag ctccccccat gcgtctagcg atcatccttg ccgcggttct
     1021 gacttcagtc gcagccgata cgaccttgga ccatcgggcg cctgccggga cacgcacggc
     1081 aatgcggcgc ccaggggcac acaccattct ccccggcggc aagatcatcg aaccgctcgg
     1141 gctccagtac cacaccggcc cggggccttt cgggctagcg gtcaacccga aaggaagcat
     1201 cgttgtgtcc gctgacggag gacctcaacg atattcgatc acagttctgg agaaacggaa
     1261 agagcgctgg cgtgtccggc agtacgagac cgaacgtagc gctgaacacc agaatgacga
     1321 agactggcgt agcgtcttca tggggctcgc ctttcagagc gagcgggagc tgttcgcctc
     1381 ggaaggcaat tctggccggg tgcggcttct cgatccccta aagggtaagg cccggtgggt
     1441 gatcgacctc aatcaaggcg aatacagcga cagctacaca ggcgatctcg cttacgacga
     1501 aggccgccac ctgctttacg tggtcgatca ggcgaacttc cgcattgtca tcgtggacac
     1561 gcgccggcgg caagtcatct cttccgtacg tgtcggccgt ctgccgttcg cgattgccct
     1621 cgctcccggc gggaagcgag cctacgtgac caacttgggg atgttccaat atcagcccgt
     1681 tcctggagcc gacccaaagc gcgcgcgtga aaccggcctt ccgttcccgg cctttggttt
     1741 cccgtctcca gaagcattgc acggcgtaaa gcgcgaaacc tccctcggtt ccgtcgacgt
     1801 cccagggctc ggagatccca acgtacccga gtcgaattcc ctctgcatcg taaacgtcga
     1861 ggacccggcg aagcctcgca tcgagacctt cgtgcgcacc ggcctgccat tcggcgggaa
     1921 gagttccggc ggtagtagcc cgtctggtgt actcgcagcg gccggccgga ttttcgtttc
     1981 gaatgcgcac aatgacacga tctccatcgt cgatcccgca acgaacagcg tcgccggaga
     2041 gatcccgatc cgcattcccg gtctcgaaag cctgcggggc gtgcttccac tcgggatggc
     2101 gtaccacgag gcggcatcct ggctcctcgt cgccgaagcc ggcataaacg ccgtcggtgt
     2161 gatcgacacg cgggagatgc gattcctcgg gcacctgccg gcgtgctggt tcccaacacg
     2221 cgtgcgcatc gatcgggata acgtgtacgt ggccaatgcc aagggccatg gcaccggacc
     2281 gaacgccggc ccgcgctcgg cgctgcgcca gagtttccag gcgctcctgc ggcgcggcac
     2341 aatctccatc tacccgctgc cgggccgtga cgagttggag cgccagacga gtcgcgttct
     2401 gcggctgaat gggtttgttc cgatcaagca agcccgggcg aaactgccgc aggccgtccg
     2461 gcacctcgtg ctgatcgtca aggagaaccg cactttcgac gaaatgctcg gcgacgtccg
     2521 gacggcgtcg aacggagaag tgcggggagc tccggcactc gcccggttcg gacgcttcgg
     2581 ctgggtgcaa caggaccgcc gggagctccg cactcggctc gccctgcggg atattgacgt
     2641 cgcaccgaac caccgcgcca tcgcgacccg atgggccttc agcgataact tctatgccga
     2701 ctctgaggtg agcgtcgacg gccaccactg gctggttgga tcctatccca acgcatggac
     2761 ggagagctcg ctgatggctg cctatggcgg ggagaaggac ttccgaatgc cgacggccgc
     2821 ccccggccgg ttgcagttcc cccaaagcaa ttcctccgtg caccctgagg aacaactgga
     2881 ggccggggcg atctggcacc atctggaacg gcacgggatc agctttcgca attacggcga
     2941 gggattcgag ttggcaggcg tggatgaggg tgaggggctg aagcccaccg gcgcgcgcta
     3001 tctcacgaac gtcccaatgc cggacccgct ctatcgcaac acctcacgcg agtacccgca
     3061 gttcaacatg aacatcccgg accagtaccg cgcgtcgcaa ttcatcgcgg aggtggagcg
     3121 gaggtatctg aaaggcggag agccgttccc gcgtttcatc tttatccacc tgccgaacga
     3181 tcacatggcg cggccgcggc ccggcgacgg ttaccccttc aacgcctcgt acgttgccga
     3241 caacgacctg gccctcggcc ggatcgtgga attcctgtcg cggtccccgt ggtggcgtga
     3301 gatggcgatc tttgtcaccg aagacgacgc ccagggtggc gtcgatcaca tcgattctca
     3361 ccgcaccgtc cttctcgtgg cgagtccttg ggccaagaga aactatgtct cccgcgagaa
     3421 ctcgagcttc ccgggccttc tcaagaccgt gttccggctg ctcgatgtgc cgccgttgaa
     3481 ccttttcgat ggcgccgccg cggacctttc ggattgcttc accagcgaag cggatttcac
     3541 tccgtatgaa gtgaggcccg tcgacccgcg gctgttcgac ccggcaaccg ccagggaacc
     3601 gctcgacccc acgccctcgc cctcgatgga tgatccccgc ttcctacagc aacaacacag
     3661 caaccgatga cagcgcgcat gtagggttct tcgtgctctc cgtggtaagt ccagcagttt
     3721 ctccggggtg ccggctccgc taaactggcc cagaggactg acatgatgct cgaagacctg
     3781 cgcggggagg ttctgaaagc caaccttgag ctggtgcggc gaaagctggt gatctacacg
     3841 ttcggcaatg caagcggcat ctcacgggag cacggactcg tagcaatcaa gccgagcggc
     3901 gtcccgtttg aggacctgaa gccggaagac atggtactga cggacctcaa cggaaaggtc
     3961 gttgacggaa aactgcggcc ttcttccgac ctggcgacgc accttttgtt gtaccgcacg
     4021 tttgaggaca tcggaggcgt cgtgcacact cattccagct atgccaccgc gtgggcgcag
     4081 gcggagcagg aaatcccctg tttcggcacg acgcacgccg actacttctc cggagcagtg
     4141 cctgtcaccc gcgcgttgcg tcccgaggag atcgagaaag actacgaaga gaacaccggc
     4201 ctggcgattg tgcgccggtt gcgtgacatt cacccgctcg acatgcctgc cgtgctggtc
     4261 gccggccacg gcccgttctg ctggggaccc tcagccacga aggccgctca gacggccgtc
     4321 atcctggaag aagtggcgaa gatcgcctat cgaacggcga tgctggaacc gatggcttgg
     4381 ccgatctccg gcgcacttct gaagaagcac ttccaccgga agcatggtcc gacggcgtac
     4441 tacggccaag aacggaaatc atgaacggga tccacgggat caaactctgc gccgccgcgg
     4501 ttgctgtgct ggcgggagct gtggacgctc gctgtgcgac ctactacgta gactgcgccg
     4561 gcggcagcga cactaacatc ggaacgtcgg tcaccgccgc gtggcgaacg cccgccaagg
     4621 tgagcggcat aacgttcgct ccgggtgact caatcctcct caggcgcggg accgagtgcc
     4681 gtggccagct ctggcccaaa ggttccgggg cccaggacca tccgatcacc atcggtgcct
     4741 atgggagcgg accgcttccc atcatacggg gcgaagcaga cgctccagtc gtgaaacttc
     4801 acaaccaaca gcactggcac atcgagaaca tcgaaagcag cggcgggtcg ccgtacggca
     4861 ttttcatcag cggcgaccgt ggtgtacttc gccacttccg aatcagggac gttgttgttc
     4921 acgacgttgg cgggacggca aagacgaaag actcgggcct gatcgctgtt gtcgctggag
     4981 gcaaagacca gacgttcgac gacgtggtca tcgacggtgt caccgcctat aacacgacgc
     5041 aatgggccgg aatcgtcgtc aatggcgcag cgttcacacg cccgcaaggt tccaccgtcc
     5101 gcgcccgcaa cgccacgatc cgcaactccg tggtacacga tgtctacggc gatgggatca
     5161 ttctattcca ggtttccaac ggtttgatcg agaagtgcgt tacgtggaat accgggatgg
     5221 agccgacgca gacaatcgga acgccgaacg caatctggac gtggaaatgc aacacgtgca
     5281 ccgtccaatt caatgagggt ttcttcgccg actcgcccgg tgttgatggc ggcgtctacg
     5341 atatcgactg gggtaacgag aacaatatca tccagtacaa ctacgcgcac gactcacagg
     5401 gctactgcgc ctcggtgttt ggagctggca atctcgtcac aacgaacagc gtcgtgcgtt
     5461 acaacctctg tgtgaacaac ggtcgcagtc cccgtctggc aaagcggcag ggagagattt
     5521 tcctgtcgac gtgggatgga ggaaagctcg acggcgtccg gatctacggc aacacaatct
     5581 actggaaccc gccggtagaa gctccagtgg tgttcgacca ggccgactac accggtaaga
     5641 aaccgaactt cttcaagaac aacctgatct attcgactgt acgaagcctc gtgaaaagca
     5701 cctcttcgat gaaactggat cacaaccttt actggtacgg cggaacggga gaaccggagt
     5761 ggctgtttgg cggcactctt caccgtggtt tcgcgaggtt cggtcaagca acggggcagg
     5821 aggcgcacgg atccttcgca gagccgaagc tcgtccttcc cgaactcacc ctggccgcgg
     5881 actcgcccgc ggtgaacgcc ggggatgatg tcgggaacat gggcaagcac gatctttcgg
     5941 gattcccgat tccccagggc ggcgcgttcg acatcggagc cctcgagtac accggtccga
     6001 tggcgaccgg ttcttccggg cctgcaccgg ccttcgctct gaaggacacc gccgggtttg
     6061 cacacacgct gggccaattc cgtggagaat ggctgctgct ttctttcctc gaacagtccg
     6121 atggcgatgc gtcagcgtct cgaactcagg ctgtgtttct caagagtgtg ctttaccagc
     6181 atggaaacca gggactgaag gtcgccgttg ttgctcgtgc cggcgttgcc ggcaccaacg
     6241 tcgtcaatca ggcccacgac tggcgtctgc gggacattcc cttgctgctc gacgaatcgg
     6301 gttccgttga acgcggctac ggagtgacca cgaagccctc gacgttcctt attgcacccg
     6361 cgggccaaat cgtcagacgg tggaacggtt tcgcttcggc cgcacagctc ggcttgacac
     6421 tgcggtggct gatcggcccg ccgccaggca caccgccggt cagcctggcg agatagcgtc
     6481 cgttatcccg cgttgttcga gcggacctgt gccgctatga ctcgcagttc aggcaacacg
     6541 tccccgatgg gaatggcatc ggagccgggg cggccgaaac cgaaatagag cttccggtac
     6601 agcgagtaga gtctctcgca aaccgctgcc tccgcggcta caggctccac ggtccgatac
     6661 ttcgggcaca acgcgtcctg cgcttcctcg acagtccgga agactcccgc tgccagaaag
     6721 gcgaagatag ccgatccgag gctcgtcacc tcgtcctgtg gaacgagcac cggtttgttc
     6781 agcacattgg catagattcg gttcaaagtt tcattcttct gagggacgcc gccgccgttg
     6841 ataactcggt tgatcggcac gccgtgttcg gccatccgtt ccaggatgac ccgcgtgtgg
     6901 aaggcggtcc cttcaatcgc ggcgaacagc tcgtcctgcg ccgtgtgcgt gaggttccaa
     6961 cccaatgtga cgccgccaag ttcggcgttc accagcaccg ttcggtcacc gttatcccaa
     7021 gtcagccgca ggaggccggt ttgtcctgct ctgtactggt caagtccgcg agagagttcg
     7081 gccacattct ttccagccct gcgtgcaatc gcgtcaaaaa tatcacccgt cgccgagagc
     7141 cccgcctcga tgccccagca ctgcgggtga atcgatcctt tcaccacccc gcagaccccc
     7201 gggatcaggt ccacatgctt cgcgatggcc atgatgcagg tcgacgtgcc gacaacgttc
     7261 actacgtctc cctcgcggat gcctgcgccg attgcgtccc aatgcgcgtc gaaagcccca
     7321 accggaatcg gaatcccgga cttcagcccg agtctcgcgg cccactccgg cgtcagccgt
     7381 cccgcgatct gatcggaggt cgcgtagcag ccgtcgatcc tccctcggac gccggccagg
     7441 agcgggtcga ccgtcaccag gaattcctcg ggtggcagac ctccgagttc ttcgttccac
     7501 atccacttgt gccccatggc acagatgctg cgtggcacct gccgagggtc gttgattccg
     7561 cacagcactg ccgcaaccat gtcgcagtgt tccagtgcgg tcgcgagtcg gccgcgcttc
     7621 tccgggttgt tccgcagcca gtgaagcagt ttcgagaagc cccactccga ggaatacacg
     7681 ccgccgcacc agtcgatagc cgccagcttc tcccggcgcg cagtgttcgt gatgagcgcc
     7741 gcctctcctt tggcacggtg atcgcaccac aggtaataat cgtcgagcgg gacgagatcg
     7801 tccccgaccg gcacgacgct cgatccggtc gtgtcgagcg cgatcgcctc gacatcacgg
     7861 ccactgaccg cggcggcttc gatggccttc ttcatggcgg cggccagccc gtccatgtgg
     7921 tcctggtgcc gctgagtggc gtgatcggga tcctctttct ttcggaagag cggatagtcg
     7981 gccgtggccg acccgatccg gcccttttca ctgtcaacga tggaaacacg aacgctcagt
     8041 gtgccgaagt cgactcccgc aacaatggcc atgcttgatc tcccgcaggc tacttatttc
     8101 ttccgacagc ttccggacct cgcaagtgac gtactgtcac gctttgtgcc agtagacttc
     8161 ccatcccatg tagcgcttcg ccgcctcgtg aaccaccgac gccgtcgaag agaagttggc
     8221 ggcgacatgg tgctcgaacc cgttctcaca gatatagcgc agcagatcct gcatcctcgg
     8281 gatctccact acgccggccc caccaaaggt ctcgagcgga tcgtccgtga atcgcccctc
     8341 tccggtgtaa caggcaatcc tgccgcagcg gtcgttcgtg gaaacgcgga agtacgtcat
     8401 cggcccggac ttgacccggc ccacacaggt tccaaacgtg ttgagtttgc ccaccgatcc
     8461 agcaatgatc tcctggtaat ccatgcgaac gtccgcgaag aagtgcttgg gcaggttgct
     8521 gcagtggaag cagactgcct tgttcgggtc gtcaccgtag ttgttgttcc agtcaagcag
     8581 cgcgctgggc gtctcggaag ccagcgccat tgcatacatc gccaccgtgc cgcacacgtc
     8641 gacttcgcac gcgctcggca tcagacggtc gctcatcatg ctcatcaccg tgcaggggac
     8701 tacgccgaaa tactcctcta tcgaagtcca gcactgcacg gcgctgacag taatgtcatt
     8761 gctctgcatc cagccgtcaa tgacggcgcc gagcttcgcc atcttcagta gcgactcgct
     8821 cgggacatcc tttgtcgaga cgtaggactt gatggcctgc aacttttcct gggccgccgg
     8881 gtcgtcgtcc ttcatcttct ccactcggcc gagaacctcc gagaggtcga gcggatcgac
     8941 gtcgataccg caggcctcga gcagtttctc gctgtaccgg accgtgttga aagccgccgg
     9001 tcgcgcgccg atcgctccca cccgcgcctt tcgaaggccg ttcaccaccc ggcaaacagc
     9061 ggcgaaccac tccaggtccc ggcgaaatac ctcggagtcc ggtctctcgg tgtgcagcgt
     9121 cgtgagcgag taagggatgc cgaactgcgt caggttgttg cagacggaca tcttcccgca
     9181 gaaactgtcc cgccggtcgg cgatgagcat tttgccggcg gtgtccggcg tcgcctggac
     9241 aagcaccggc acgttcagcc cggccatgcg cagggtctct gccactcccc gttcatcgcc
     9301 gaagttcggc agcgaaacga taacgccgtc gatggaatct cgatgcttgc ggaacaggtc
     9361 tgcgcatttc cttgcttcgg cccgagtctc gacagcccca tacttgctgt catccggtcc
     9421 gagcacaacc acactcatcc cggcgccctg tacggccctg atcatctcct cccggccgga
     9481 cttggcgagg tggtccggga agaacccccg attgccgatg attaacccaa acgtcatctg
     9541 tctccgcata ataagctcca attgtatgta atcccatcgg cgtcgtccga agtcaacgct
     9601 cttcgatgat cgcgacgtcc cacggcggca acgttacgct ctgcgcgcca ttgacggcgc
     9661 ggccggtcag caggtccgtc ccccccgcgt aggagtaaga aaaagtctgc ggctggccgg
     9721 agtaattcag aaagtagtgc atgcgcttgc cgcgatttcc cgtaccgtgc tttacccgca
     9781 ccgccgccgg cagtttttga tcggagccaa gcagacccgc caatcccatt acttccgcca
     9841 gcagcttctc ctgaagctcg tcggagaggt acgtcccttc gtacgtcagc gtgcctttgc
     9901 cgtgcttgtt ccgggtcaat gccgggaagc tcccaaaaaa cggatggtcg tatgtggcca
     9961 gtggtgtcgc ggtttccggg ataaggaact cggcccaggc ggacactctg ttcctttcgc
    10021 ccgctccata aggatcgccc ttcagcgcca gaggctctgc caagttggaa aactcctgat
    10081 agtggaagcc ggctgcctgc cgcagcggcc ccggcgccat gacccagcgg accgtcgagt
    10141 gctcgttcgt gaagccgctc ttaaacgcca tcacaagatg ccctccgtct ttgacgtagt
    10201 cagcgagccg tgtcagcagt tcgtcgctgg cgacataaag cggcggaacg aggatgatct
    10261 tgtagttcgc gagcttcttg ctctgtggaa acacgaagtc ggtctggaca ttgagccggt
    10321 acagcgcccc gtacatctgt tgcagtaccg ccatgtagtt cacgcggtcg tgaaacggca
    10381 tgaactggat gccgtggtgc gagtcgatgc tgtacaaaat cgcgacgtcg ctcttcttct
    10441 ttagattcgc aagttggggt ccgagttttt ttagttcctg cgcgattcgc gtcacctcgg
    10501 tataggctcg gttcggttcc agatcgtggg agaggattcc cttccagtag gtctcctgtc
    10561 cgtaatgaag ggaatgccag tgccagtagg ccaccatgtt tgcgccgctc gcgacatggc
    10621 tatacacgct caaacgtagc tggccgtcgt acggcggata ctgcgttctg gaatcccagc
    10681 cgatcgtctg tgcgttcgtc tccgtgacca ggtagttcgc ctgcttgagg gagcggcaga
    10741 catccccaga caaactgatc gccaacccat ctagttcgtc ctgcaccgcg tgatacggat
    10801 tgacggcggt gatatcgagg tgctccgcga tgtcgaactg gttcacgtca gcccgtacgc
    10861 cgccgacaaa gtcatgcgtg atgaactgac cggggcgctt gtactccttg acgatggccg
    10921 cttgccatgc caaaaagtcg gtgaccgtct tctgctggta ccgctcccac tccagcttgt
    10981 agcctggatt cagaatgcct tcacgcgggg gaaactcgtc ccaaccgttg accagttgtc
    11041 cccagtaggc aaacccccag agcttgttca gcgcgtcggt tgtcccgaac ttccgcttca
    11101 ggtaggtaac gaaatccgtg tgcatgctga gcgagttcgt gctgttcggg tgggtttcgt
    11161 tgtcgatctg gtagccgatg accgccggat ggtccttgaa gtgggagacc actgcgcgga
    11221 tgacgcgctc agcgtaccac cggtatgtcg gatgggtaat gtccatgttc tggcgcgggc
    11281 cgtaggcccc aggcaccgcc gcgccgggat acgtcgcctg ggtcggatgc gtgagcggcg
    11341 gcgctttcgt aagttgggag accaggatct ccggatgttt ctggaaaagc cacggcggga
    11401 tcgaatacgt cggcgtgccg aggatcactt tgatgccggc cttatgcaac cggtctatga
    11461 tccggtccat ccaggcgaat tcgaattcgc cgtcacgggg ttcccaactg ctccacgtcg
    11521 attcgccgac ccgcatcacg gtgaggccgg ccttctccat gagttggacg tcctgctcca
    11581 gccgctcata gtgcatgtac tcgtggtagt acgaggcgcc gtaaagaatc gtgtcgatct
    11641 tgggtgtctg ggcctgtgcc agtaagggga caaggtagag aagcagtaat agccggggca
    11701 tggcaaggat ctctgaagat atcaaactgt ggggcgctcg cgtacgcggt gccaggggcg
    11761 ggtcgggacc cgccccccaa gcacccctac cattcgaatc gtgcgccgaa ctgcccctcg
    11821 cgcggggtac ccgtcaggtt gatgacgtgg ccgaacgtgc ccgaactgat gttcgtgtcc
    11881 ggcgtgtcga aatggtggcg gttgaacacg ttgtagaact cggcccggaa ttgcagccgg
    11941 aaccgctccg cgattcgaaa gttcttgagg atcgcgacct gctcctcgga ccagccgaag
    12001 cctcggaaat ccgagcggaa catcgggacg gtgccgaggt ccccatctgg cgggttcgag
    12061 aagttcttcg ggtcgaagta gaggttgccc ggtaagcccg ggtcgccgaa gttgaagtcg
    12121 gatccttcga agttgcggct gtagtcgccg ttctcgtttc tgagggcgta gaccggcgcc
    12181 tcccatcccg gataatagtt gctcgagttg actccgatcg gcgaaccgga gttatagcgg
    12241 aaactgccgc tgagcgtcca gccgccgagg atggcgttgg ccacccgtcc tgctccgttg
    12301 aggtagcgcc gccctgtgcc gaatggaaga tcccaggcaa cgatgccttt gaacacgtgc
    12361 ctttggtcat agaacagcgg cgccttggct tcttcatcga ggtgcagcac gtcctgaagc
    12421 gggcccgacg accagatctc ctggaaaccg cccagagagt tttggaggtt gctgtgcgaa
    12481 gtcgccaggg tgtagctgac gttcgccgtc aggccgcgtt cggtacgctt cgtcacggca
    12541 agttgaaggg ccttatagtc cgacctcccg tacggggagc cgacgtgaag cagcggcccg
    12601 ccccacaccc agttctgcca gatctgcggg tatggcatga tcgccgcaaa cgcgtaggag
    12661 ccgaagccct cgaacggaaa cggaacgcct gcggacgccg ccgagccggc atcagacacg
    12721 gcgtcccaga cgtggcctga cctgatgagg tttgccatcg cacccggatc cggctgattc
    12781 cgctcgaagt cgccgctttg cagccggcgg ccttcgttct gcatgtaggt cacgtccagc
    12841 cgcaagtcgt tcgtgagcgt gaactgcgtg ccgatgttcc actggtggct gtagccggca
    12901 aagagactct tcggatttac gttgaccatg ccccactggg tgtagtccgg atcctttgtg
    12961 ccaggcacga acgtgccggg ataccccacg tcccagttga aagccggcga cagatcggcc
    13021 gtgggagtca cacggttggt tccgcggtag cctggcgcga acccgtatgg cacgccgaac
    13081 cagtagttca tcccgatcgg cgaatagaac agaccgtagc ccgcgcgcag gacggcacgg
    13141 ctgccgattt ggtaggcgat gccaacgtgc ggagagaact ccttccagtc gcgttccgtc
    13201 tcaaatgtgt cgtctccgtt cttggcaaac acgagggttc cggggatacc aaacgactcg
    13261 ctgattgctt tcttgtcgaa gttcgcccac cggccgtatt tctcgtgata cgggaacgtg
    13321 acttcccagc gcaagtccat cgtcaatgtc agattgcggc tgatcttgaa gtcgtcctgg
    13381 gcgaaaatcg agaacgcctt tcgcctgccg taaaggtccc acggcgtctg ctggctcgct
    13441 tctttcggcc cgccaagtag gaaactggcg aaaccaaagc cgaccttgtc ccgccaggcc
    13501 tccttcggag cgccggtctg gtcggggtca aactggaatt ccaacgtcgg cgtggactgg
    13561 tggctgctta actgaaatgc gcgaaattcc ccgccaaaac gcatcgtgtg acgccctctt
    13621 atccaggtca gcgattcgtt tccgatgaac gtattgccga cgtagtagtc ggaccaatgc
    13681 tggccgatcc tggttgtgtc aatcccattg atcgcacggc cgaattcgat gactggaaag
    13741 ttccccgcgc cggtttcgcc gaagccgagt ttggagggcc agtcgtcctt cgcggcaaca
    13801 gaaaagctgg gattgcggta acggccgaac gtgaagctgg caatgttcag gactgtcggc
    13861 gagaagttgt aggtgtgatt tagcgcaaac ctgcggctgg tgacttcctg cttgcgggag
    13921 ttcgccatcg gtccgcctgc cttgtccgga tcggctgggt cccagattcc gccctggtcc
    13981 accagaatgc gcggcctctc tgtccagacg aagtgcccgc tcagccggtt cgcgtccgag
    14041 aagttatggt ccgccttgaa cgtcaactga gtttgatgga accacggatc attttgccgc
    14101 gtgatggcag tgttgttgat cagccccggc cgcatcggga gatagcgctg ccggaagacg
    14161 tcggtgatgt tctttgagat agtgctgatc cggttcgacg ggatcacgtt cgccgcgctt
    14221 ggtatcggag caccggtaac cgggtcgaag ccaaaccctt ccctgaccat cccggtccgc
    14281 gcggcggtca gcccggtaac cgggtcgacg ctgcccgccg tcacgttccg ctgagtggcc
    14341 gggtcgtaga tcataccttg gtaaaccgga cggccaaggg catcctggcc aagcacgcta
    14401 cccgtattca gcagctcact gaagttgccg tcgaggaacg ccgggagggg cacagtcgcg
    14461 ttgtaggcgc caagcacgaa cctctcctgg gtgtatttct caaaagcgcc gaacgcaaac
    14521 gtcttgttcc tgataatcgg cccgccggcg ctgaaaccgt acacgaactg ccggtccgtg
    14581 gcgcgcttgt ctccctggaa gttgctctgc cacgtgttcg cgttgaggac ctcattgtgg
    14641 aggaagccga acgcgctgcc gtggaagtca ttagtgcctg atttcaacgt gaacttgaag
    14701 acgccgccgc ccgtccggcc gtcttcggcg cggattccgc tcgtctcgac cttgaattcc
    14761 tggatcgcct ccatgctggg gctggactct ccgatgtgcc caccgatctg cacgaccgcc
    14821 gaggtgccgt cgatcagaac ctccttggtg aacgccatgc tcccgccgat gtacgaagtc
    14881 cagttgttgc cctcgacccc ggggatggtg gcgtaggcga agttctccaa cgaacggccg
    14941 ccacgaatgt ccagcggcag gtccgtcact aagttgctgt tcatcccggc cccgacctgt
    15001 gtgctctgcg tttcgagcaa ctgggcctcc gcggtgacgg tgaccgcttc ggccaaggcg
    15061 ccgacttcca tgttcgcgtt caactgaaca acctgcgcca ccgcgatgct gatcccgtcc
    15121 cgcgtgtaag tcttgaaccc ctgcttggtg aacgcgacgg agtatctgcc gatgggtatg
    15181 ttgagaacgg agtacagtcc gacctcattc gtcgtggcgg tgaagaccac cccggtgctc
    15241 aggttcgttg ccgcgatctg aacgccgggg actaccgcgc ccgtcgcgtc cgtcacagta
    15301 cccgtgattg cgccccggtc cgactgcgcc gaaagagcgc cgatgaatgg cggcagcgcc
    15361 gccagtagaa gaaggatggt gagccttttc atcacacgat gctcctggcc tctggccgct
    15421 cggcctgcgg gaagtgtgga catgcccccg cccaaatcgc gcctggaaac agctatcacc
    15481 cccacttgag cggcctgatt gtaagcgttt ccattgggag tctatcacga gatacaaacg
    15541 gtttcaatag aaaaatgcac ctgtgctcga ctcaaatttg actaccttct gtaagcgttt
    15601 cctcgagggc cgctaaagca gagttacaat ggtgggtcaa ctgaagccca gttttcatcg
    15661 gttcgggatc ttccgccatg acgctagaag aggttgccag acgtgctggg gtctccaccg
    15721 ccaccgtctc ccgggttttg aacaaagccg gcgtcgtcag ggagacgact cggaaacggg
    15781 tcctccaggc cgccagggaa ttgcggtatc accccaatct ccacgccagg accctggctg
    15841 gagccaagag caatactctg gggatgatcg tatcgaacat cgagaatccc ttcttcatcg
    15901 acatctttcg cagccttgag tcggcagccc tccagagggg ctacgaggtc gtcgtggagc
    15961 ataccgacta caagtcgagc cggctggtga cctgtgcccg cgcgatgttg ggccgccgcg
    16021 tcgcgggcct cgcggcgatc gtttcggaga tggagccctc tcttatcgag gagctcaccg
    16081 aaagcgatgt gccggtcgtc ttctatgatg ttgggctccc ggcccggaac atcaccaaca
    16141 tcagagtccg gtacgagcgc ggcatgcagc gcacagtgga gtacctctac tcgatgggcc
    16201 acaggcgaat ggcgttcgtt gggcaccacg cgagcctggg cccgcttcag gcccgcaaga
    16261 agacgttcat cgaaaccatg gcgcgctact cagggcaagt tcaattcacg gctgcgctca
    16321 acaccgacag ccccgccggc ggtaaacagg cggctcacga actgcttgcc tccgatttcc
    16381 gcccgacggc cgtcctctgc gtgaacgact tcatggccct cggagtgatt cgggagttgc
    16441 gcgaccaggg gttctcggtc ccccgggaca tctccgtcac ggggttcgac aacatccagc
    16501 tttcggagtt ctcaaacccc gctctcacga cagtgaatat tccgcgccgg aagatcggcc
    16561 aaatggcgtt cgaagccctg gccccgaagg agagttccgg cgatcgcccg cgcgagttcg
    16621 tgatcgaccc ggagctgctt gtccgggagt ccaccggacc ccctcccgtc tgagaacagg
    16681 agactcggaa atcatgaaag tactgaagcc cctcttcata tcgttgttgc ttgtcttacc
    16741 cgtagccgcc cagttgccgg acttctacag aaccgtcgac cgcctgatct gggtggtgga
    16801 cgatctcgac cggaccacgc agggctggca gaagctcggc ttcgctggca tcgagtatca
    16861 gggcgaagcc gaactcccgg ccttgttccg cggcaaggcg ggaacggcaa ctcttcgtgt
    16921 ggcctctggc cgcatcggcg acgtccccgt ccactgggtg cagcctattt ccgggaaaga
    16981 cgcgttttcc gagttcctgg cccggaacgg cagtggcgtt ttcagcatca tgcaccgcgt
    17041 cccctcccgt gaggcgtttg acgccgaggt gaaacgaatg gcggacattg gcgtcggcgt
    17101 ccttcaggga ggtgaggcgg agacagaagc tggaccggtc cgtttcgcgt tcctagatac
    17161 cgccgcgcga ggcaagtacg ttctcggcct gcttcatgtg ccaggtgcgg cggatctggc
    17221 gctcgcgcct cccggaaacc cctccggccg gaaggtcgcc cagtacgcct ttgtggccaa
    17281 ggatctcgac gccgtctccg cgtattgggc gcggctcggc ttccccgcga tgacgtacac
    17341 ccatcccgat tcgtccgagt tgcgctaccg cgagcgtccg ggggagttcg atatgaagct
    17401 gggctggcag cgccatggca aagtgaccta cgagtggatc ctctcgctga aaggtcccaa
    17461 tgtttacgag gaccacatga agaagcacgg cgagggccta caccacctgg ccttcgaggt
    17521 cgaggacatc gacaaggaag ccgcggactg gcaggccaag ggattcccgt tcggtatggg
    17581 cggtgcgtgg ggtcagaagg gcaagcctgg ctcggggcgg ttcatctaca tggatacaca
    17641 cgccatcggc ggcatcgaca tcgagcttct ttggagtttc cgggcgaagt agctttttcc
    17701 gccggccgaa gacaccctct cctcagccgc ctgagtaacc tccggccccg tgccgagcgt
    17761 ctttattccc ttggccgggt ctgccgtcct gtatactagc aggcttccgg cacgattccg
    17821 gagaagacgg tcacttaatc ttccaggagg cagtcttgag ttttcaggga tggcttgccc
    17881 tcattctcgc cgcggcgctg gttgcgccgc cggtcctgtc cgcacaggag gctgccccgc
    17941 ctccgccaaa acagtccggc ggaactctca aaatcgttac cctcgaaggc gagggcgcga
    18001 agaacgtcat caagacaagg tatgccgtgc agccggtagt cgaggtgcac gaccaaaacg
    18061 ataagccggt atccggcgcg gaagtcgtgt ttcagctccc cgcggccggc cccggtggtg
    18121 tttttaacgg atggatgcgg acccagacgc tccgtaccga cgccgaaggc aaggccggca
    18181 cgaacggctt cacaccgaac gacgaagaag gccgctttaa catcaaggtt accgccacct
    18241 caaacggcaa gacgggcagc gcgatcatcg cccaaagcaa cacgcgaacc ggcggccagt
    18301 cgtcctccgg tgcttcgaaa tggtggaaag tactggcgat cgccggtggg gcggccgcgg
    18361 ccggcgggat cgtggcggcc actcgaaacg gcgattcttc cggtgccggg aacaaccccg
    18421 ttacgattgc accgggcacc attacggtga ccggaccgag gtaagcgcgg gtgctcctgc
    18481 gggaaggctt catactcata tgcgcaatct aaaattcttt ccgtgtggcc tgctgctact
    18541 cggcttgggt gcagcattcg gtcaggatgg ggggctcaca ggccccgtct caggcatggt
    18601 attcgacgcc gactcccggt ctctcaggcc ggtggtcggt gtgcccggag ccgcctatct
    18661 cggccagccg gcggtttccg gccttgattg ggcctcggta gcgcccaatg gccgggcagc
    18721 gctcgttctc gggaatgctc agcttcgctt actgcgctgg tcctccgacg ggaccagtcg
    18781 cgccgacatc atcgtcgaag aagcgcaaac gcccggacgt gttgcctgga gcgccgactc
    18841 gtcggcggtc gcggtctatg agaattccgg gcggctgcga gtttggcgca cacagactgc
    18901 ccagcccgcc ggcgacatcg atctgtcgtt gctgtcgggc cggatttccg ctctcgccgt
    18961 cgagaagtcg ggcgctgtcg tgatcggcgt ggaagatgca acggcaggcg gggtattccg
    19021 ggtctcgccc ggcgccgctc cggtaatggt cgcagcgatg gcttctcccg gagcgatggc
    19081 cttctcgaga aacggtcggg acctcttcgt ggccgatcgt gccacccgcc gggtgatcga
    19141 aattcgcggc tatcgcgcat cggcggcgtt gattccgctc gcagacgaga cgagtggggt
    19201 cgtcgagccc gccgcactgg cggtgtccga agacggcatg cgcctgtttc tggccgacag
    19261 gggcaagaac accgtgggca tctgcgatct cacgacgcgt acactcgccg gagagatcgc
    19321 actcgatttc gccccggttt ccctggaacc cctttctgga caatccctct acttgttgaa
    19381 aggcggaggc gggggtgaac cgttccaggt gttgagcgcc ggccgggaac cggcggttta
    19441 ctttgtgccg gccgagcggc tggtcctgga ggagcgctag tcatgtctcg aaccaccctc
    19501 gtcctagttg ccgccgtcct gatttcgttc ctgctggctc ccgccgccct cgcgcagtcg
    19561 gtgcccttca ccttgcgtta cgaccagggc gacacgacca cggtcgtgtc gaacaatagc
    19621 agtgtcttct tgccggccga cgccgtcgga caggcggcag tggccactct aaccgtcacc
    19681 taccgtggaa ccacttccgt ctccatcacc gaggtcgaga tgctggacgc gttcgagttc
    19741 ccttcagccg acttctcggt cagcggcctg ccgtcgctcc cgctttctct taccgagaac
    19801 cagagtttca gcgtcaccct ccgctaccag gcggcgaccc gcaaccgcgt tatcggacgc
    19861 gtgttgtttc actataagca agcattggtg accagccaga tcacgctgaa tctcgtcggt
    19921 gtcgcgccgg aattctccta tagctacata ccgcaagggg gcaacccggc agttttggcg
    19981 cccggtggga ccatcgcctt cctcacgacc gcggtggacg gagcgtcgac cgctactgtg
    20041 gtaatcacca acaagggaag cggtgcgggc aaagtcgatg ccatcagttc ctccggcgct
    20101 gcgtttgagt tgagtggcct gccccttccc ccggcaacca tcaccgccgc ccgcgaactt
    20161 cgcttcaccg tcacctacaa accaacggcg ctcgagacaa gcagtgggca gctcactctg
    20221 gacctggctg gcggccgagt tacgttcgcc ctccagggcg tctccgaagg gcccgcgttt
    20281 gtttacgaga agatcgaaag ttcggcggtc gttcccgtaa tccccgggca ggttgtttcg
    20341 ctccccgata ccttggtcgg tgaggagact agcgtgccct tccgggtgag aaacgatggc
    20401 aaccacgacg gccagattgc gtcgattagc gtccttggcg acggttatca ggtaactgac
    20461 ctgccgttcc tgccgctggt tctcaaaccc ggagcttcgg cgacgttcac tgtcaagttc
    20521 tccccctcgc agcccggcaa gatttccggc aggctgcgcg tcggcgacga cagttttgac
    20581 ttggccgcat cgggtcttgg agcggcgctc gagtattcgc tcgtggtcgg ctcgacgacc
    20641 accacggtac caagcggcgg catcatcacg ctcccgcagg tggcggtcgg agaaagcgta
    20701 aaggctcgct tcatcgttac caacacgggc acggcagagg cgtccatcaa cagcatcact
    20761 ttgccggcgg cgtcgcctgc ggttttcctg ctcgaagaac tgccatcgct tcccgcgaag
    20821 gtagctcccc aggcaacgac tggattcact gtcgtcttca aaccttccgt ggtcggaaat
    20881 gcgtcgtcca ctctcaaggt ggacaacgtc accttcacca tttccggcgc tggcagttcg
    20941 ccggcaccgc tgccggcata cacctttgaa ggcgccagcg gcgttcagga accgctccag
    21001 caacctgggg tcggcctgac gctgtccgag ccgtattcgc tcgggctgac cggaaccctg
    21061 agactggctt ttacctcgga ggctttcagc agcgacccgt cagtgcagtt ctcctccggc
    21121 ggacggacgg tgaacttcac catccccgca aactctaccc gcgctgtgtt tcccaatggc
    21181 accaacgaga tccgccttca aacgggcacg gtagcgggga caatcaccct cacgccgtcg
    21241 ttcgctaccg agggtggcat cgacatgacg cccgacgttc cagcggttct gaacttgatg
    21301 gtgccgcagt cggctccgcg agtgctcggc gtccagatca cgtcgaaaac cactgctggc
    21361 ttcaccgtgg tggtgacagg tttcgcaacc ggccgctcgc tgacccagat ggacttccag
    21421 ttcaatccgg tcagcggtga aaatgtctct acgacgaagg tcagcgtgaa tgtggaagcc
    21481 agtttcctca gttggtatca gagcgcggcg tcgcaacagt acggcagcca attcttggtg
    21541 agcattccct tcacgatgca gggtgacgtg aggaacgtga acagcctgat cgacacgatc
    21601 cagtcggtgg cggtgaccat cggaaatcgc cagggaacct cctcggccgt gacggcaaac
    21661 ctgaggtagt gatagcggtt gccccggcct tcctaccctg cgcgtcccgc gggcgcgcag
    21721 ggttttctat ttgttgtggc gtaccgccat atactggcgt gagaggtttc ctgatgagcg
    21781 catctgaaat gacacgccgg gcgtttgccg gagcagtggc cgcagccggc ttctgtttcg
    21841 cgcaggatgg ctggatcgac cttttcgacg gtcagagcct ccggggctgg cggcccagcg
    21901 agaacaagaa ctcgtggaag gtcgttggcg gccagctctt ggccgatggc ccgcgctccc
    21961 acctcttcta tgacggcccc gtccgcgagg ccggcttcaa aaacttcgaa ctcgaagtgg
    22021 aggcgctcgc acacccggcg gctaattccg ggatctactt ccacaccgcc taccaggaga
    22081 ccggttatcc acgcaagggc ttcgaagtac agatcaacaa tacagccacc ggggagggcg
    22141 gctaccggga acgaaagaag accggctcgc tctacggcgt tcgcaacact tacaagcaac
    22201 tcgttgcgga tgacgggtgg ttcaagatcc atgtcgctgt ccgcggcaag aacattcaag
    22261 tgcggctcaa cggaatcctc ctggttgact tcaccgaatt caccccgcca ttcatcgccg
    22321 cagggaatga gcgagagcgt ttcctcgacc gaggcacctt cgcgctgcaa ggccacgacc
    22381 ccggttccaa ggtccgcttc cgccgcatcc gcgtgcgacc gcttcccgac gacgtcccaa
    22441 cgcccggctc cgcgtccgct gccgctgacg ccctcgaaca ggacgtcatc accctcggcg
    22501 cgcgcaacat cccgctcgtg gattaccacg tccacctcaa ggcgagcctc acgctcgatc
    22561 aggcgctggc caagtcgcgc cgcgacggca ttcagcacgg catcgccatc aattgcggcc
    22621 aggggtttcc cgtccagaat gacgattccg ctcgccgctt cgtcgatgag atgaaggggc
    22681 agccggtctt ccttgccatg caggctgagg gacgcgagtg gactcggatg ttctcgcggc
    22741 gcgcggcggc gttgttcgac tacgtgttca ccgactcgat gacctggacc gacaaccgtg
    22801 gccgccgcat gcgcctctgg attcccgaag aggtcggcac gatccccaat ccgcgagagt
    22861 tcatggacac cctcgtcgaa cgcaccatcg gcatccttga aaacgagccg gtcgacatct
    22921 acgtcaaccc gaccttcctc ccgaaacccc ttgacggaaa ctacgatacg ctgtggaccg
    22981 acgagcacat gaggaaggtc gcccgcgccg ccgcccggaa cagcgtcgcg atcgagatca
    23041 acgaccgctt caagctccct tctgccgcat tccttcgcgt ggcgaaggaa gaaggctgca
    23101 agtttgcgct cggcacgaat aatggaggtc ccaccgacct tggccgctgc gaatacggcg
    23161 tccgcatgac cactgaactc aagctctcct ggcaggactt cttcgtcccc ggtgcgtggg
    23221 gacccaaagc agtcgagcgc aaacccggcg cgctccgggc gtaacgcaga gccctcgttc
    23281 gctaccggag cccgtcgaag agatcgctct cgcgcatacg ccccccgttg accgagctga
    23341 agacgcgata gtactcctgc gagccccaga gggcgcggtg atgctcgtcc gagagatgct
    23401 cgagtttccc atagattgcc atctggctct tgtggcacga gaccgcccgc cagactttgg
    23461 accagaaggc gctggtgtcg atgacggtag taaccgccca gttcggccac ggtgcaacca
    23521 cgcggtcggc gccgtccacg gttaccttca ggttacggaa cgccgcttga tacgccgccc
    23581 atttcgcctg cgtccacgcc atgtagtaga gcttcgagac gcggtggctg acggctcgac
    23641 ccggcccgtc ctcggcgccc ttgtattcgg gatctgccgc gcagaccacc gctgcggttg
    23701 tgaactggca gatggcgatg tgatccggat ggccgtacgc gccctcggga ccgaacgtaa
    23761 ccacaacctg cggccgtacc cggcgcagat gtcccgcgat cttgccgatc gcttccgaag
    23821 gatcggcctg gtccagatcg ccgtccaagt agtcgagaaa ccgaacctcg cgtaccccca
    23881 gctctccggc ggccgccagc agttcctcct cacgaatcct gccggctacg tcgggcgcct
    23941 cgcgctctcc gtttggcccg atgcggccgc gctctccgcg tgttgccgtt acgagatagg
    24001 tctctatccc ttcgccggcg tatctcgcca gcgtgccccc catccccaga gattcgtcgt
    24061 ccggatgggc caggacgcac atcagtctta gcgggttgga cacccagcac cccctttcca
    24121 tcaggattcc agatcaaccc agctttgtac acagctctgg ctcaaatttc cggaggattt
    24181 ttgggaaatg gctggagcgg gagacgggat tcgaacccgc gacatcaacc ttggcaaggt
    24241 tgcactctac caactgagtt actcccgctt gcctaccccg tattgtcgct ccgcgtgcgc
    24301 tcgggtgtca atttgacgaa cacttccggc cgccacctgt tgtggctcgg cgtcgccgct
    24361 tcgtgtacga tttccgttga tgctcacgca ctcgtttcgt tccgccactc tactgcctgt
    24421 cctcggcctg ctcctctttt ccggctgcac ccggcgcccg gcgtcgcagc ccgcttccga
    24481 aaccccgctt ccgagacccg gctggctcaa gaaggagccg ctgatcgttg ccggcaactg
    24541 ggacagcatg gccatcttcc ggcgccgcgt cggcggcaac ccagtctggc aggaggagga
    24601 ctacgcgcgc gaacacaccg aggaaacagt ccgcaaacta aaggaccttg gcgtcaccat
    24661 ggccgtgatc cacttctaca agggctacgg gatgcgcgcc gaaagcgagc aactggagga
    24721 ctcgcggaag ctcgccgccc tctgcaagaa gtacggcatc cgcgtcggcg tctacgtcgg
    24781 cagcaccgtg gcctacgaga ccttcctcgc cgaagcgccg gatggcatcg actggttcgt
    24841 cccgccgtac ctcggcaggc ccgtcttcta ccccggccag agcttccgca agcgcgtcta
    24901 cttcatgcac cccggctacc gcgagtacat gaagcgcgtt ctcgacgtcg cgatcaagga
    24961 tctcaaggtc gacctgatcc acttcgataa caccagcctt caggcgcagg cccctgtctt
    25021 ctatcactcg caggcggtct ccgacttccg tgacttcctc tccgccaagt acacgccgga
    25081 gatgctgaag aagcggctcg gcttctcgga ggtccgtttc atcgaacctc ccatcgtgga
    25141 ccgcgtggtc agtgtgctca acgacccgct cgttcaggag tgggccgatt tccgatgccg
    25201 ccagcttgcc gcctattacg gcgaaatgga gcgctacatt cggggcctga acccggaagt
    25261 cgcggtcgag aacaatccac actccggagt atcgggcatc aacaccgtct gggagcaagg
    25321 cgtcgactac ccgcgcctgc tcgctcacac tgatgttgtc tggagcgagg aaggcaacga
    25381 ggccggcgtg acgcccgaag gcgttttgct ctccaaaatc cgcactttca aaatggcctc
    25441 cgccctcaac aacagcatct ttacgtacac cggcggcgac cagggctcta cgcttcagat
    25501 ggctgaggcg atggtctaca accggcgtaa tctcggaatg gtcggcggtg ttctcgcagg
    25561 ctatgacacc ccggccgatc agcgcaatta catccggttc tatcgcgaca atttcgacct
    25621 ataccgcgac gcggcgagcc gctccgatgt cgccgtcctt cattcctacg cttcgctcgc
    25681 ttacaacaac gacctcccct ggcagagcac catgctcttc gagcaggcgc tcattcagtc
    25741 gaaggttccg ttcgatatca tcttcgacga tcacctgaag gacctctcga aataccgagt
    25801 tctcgtcctc gccgatcagg agtgcctcac ggacgagcaa cttgagcagg tccgccgttt
    25861 tgtcgccgca ggaggcggtc ttgtcgcgac ggaacacacc tcgctgtaca ccggttggcg
    25921 gcagcgccgg cgggactttg ggctgcgtga cctgttcggc atcagcttca gcgtgagtcc
    25981 agccgcctgg cccggctcgc gcgctgcgga acccgtgatg aaaatagccg cagtccagag
    26041 acaggccgac aagggcaggg tcgcatatat tccttccgtc gaacccgccg ttccgaagcc
    26101 ggacggagca ccaatgagga gcgagtactg gaagctcccg ctcaacatga aggaactgat
    26161 cgacgccgtc cattgggccg gcgggggtct gctgtctatc gaggtgaaag cacctccgac
    26221 ggtaaccgcc gaagtgctcg aggacacgac acacaaccgt ctgacggttc acctgatcaa
    26281 tttcgattgc gagcgggcgc cgaagctttc cggtctcaca gtaaaccttg ccatccccgc
    26341 aggcagaacg gtcgccaaag taacgtggct ttcgcccgat gaaaaagggc cgctaccgtt
    26401 gacttcgagt ctccgaaacg gccgagccgc gctcttaatc ccctcgctag agacgtatgg
    26461 tgtagcggtt ctggaactcc actagcttct ggacgacaca ggacggatat gacattccgt
    26521 gacgatcctg ttgcaaacat ttccgaagag ttaacaagac ccctacggat tgttttattg
    26581 ctctgtgact agttgattac aaacgagaaa taattgtcgg agcaacggga tctgtcgcct
    26641 tggattctgt gctagactga ttctccgctc ggctcctgag agagcggcca acgacaagtt
    26701 cgttgaatca ttcgctagcg gttccagcca gctaaactga aggtctgttt tcagatttcc
    26761 tcaggctgtt ggaaatttgt ggaaatcttg gatggttggt gtgggttgtg atctatgaac
    26821 gagtgggagc agattaagaa acacatcgct ggcaggctca gcaacgaaag ctaccagaac
    26881 tggctcgcca agacctcatt ccatcaactg gatggtgagg tccttcgcgt cagtgtgccg
    26941 aacgacgcca ccagagcttg gctcgaggag gagtattcgg acctggtgag cgcttcggtc
    27001 cacgagctca acctccgaat ccatcgggta gcctatgaac ttcctggagc gacaatcccc
    27061 gatttgaggg ccaataacgg tcatgatggc gtcgttcctg agctgatgtt ccgttctccc
    27121 gacagccacc tgaatccccg cctcacgttc gacacctttg tcgtcggttc ctgtaaccag
    27181 ttcgcgcatg ctgcggctaa agcggtcgcc gccatgcctt cgcgcaacta caaccctctg
    27241 ttcatttacg gcggcgtggg catgggcaag acgcacctga tgcatgcaat cggccgatct
    27301 ctgctcgaca acttcagcgg catgaagatc gtctacactt ccagcgaacg cttcatgaac
    27361 gagatgatcc agtgcatcgc cacgcaaagg atgtctcact tccatcagca ctatcgaagc
    27421 gccgatattc tcctcgtgga cgacattcac atcctcggag gtaaggagcg cacgcaggac
    27481 gagttcttcc acactttcaa cgaactctac gaccatcaga agcagatcgt catctccagc
    27541 gacagctcac cgaaggatat ccccggcctc gtcgagcgtc tgcgttcgcg tttcgagtgg
    27601 ggcctcatgg tggacattca gccacccgac ctcgagacca agatggcgat cctcgacaaa
    27661 aaggcggaag cggagggcat tactcttccg gaggacgtac ggatcttcat cgccacaaaa
    27721 acaaagtcga acgttcgcga actggaaggc gctctcgtaa agctcattgc ttattcctcc
    27781 atgagtggaa cgccgatcaa cctcccgatg gcacagaact gcctgcggca cctcgtccac
    27841 gggcaggaac gcaggattac gatcgagagc atcgtgcgct cggtagcgga acggttcagc
    27901 cttcagcctt ctcagttgaa acagaagagc aacgctcgtg cgatcgcata cccccgccag
    27961 gtggccatgt acctcgtgaa ggaactcact tcggcgtcgc ttcccgaaat cggccgtgct
    28021 tttggtggca aacatcacac aaccgtgctc cattccgtcc aaaaaatcga gacccagcgc
    28081 caccaggacc ctgatctcaa cagaatgctg cacagtctaa tcgattcgct gtcctgaact
    28141 tgtatgcttt tccacaaacc ctcgtcccga gatgctttgg atagctgtgg aaacccgctc
    28201 caggcgccca accgtactcg ccggaccact cggattccca cagcgagacg agacaggaag
    28261 cggttgggtt ttgtataatt tagagagaaa tcaacatttc gacagtcccg atgaattcgg
    28321 ttctaaagca gacagtttag agatctcgta gaaagcttgg gaaacggaga cttggcccga
    28381 tggatttcat agtgagcaag aacgacttgg ttcgggagtt gaacctgtct cagggggttg
    28441 tcgagaagaa gactacaatc ccgattctgt cgaatgtctt gattgaagcc agcggagaca
    28501 aggtcaacct gacggcgact gacctcgagt tgggaattcg ctgctcttgc cctgcgcgag
    28561 tgaacaaaga cggagccggc acgattccag cccgccggct actggactac gtgaggctgc
    28621 tcccggaggc ggaactgcac gtcaagttca tggagaatca gtgggcgaat ctggtttgtg
    28681 gccgcagccg gacacggatc gccggtatgt cgagggagag ttttcccgag ctccctgagt
    28741 ttcctgaagt gctcgcggag atccccgtga gcgagttgtc cgggcttatc gcgaagacga
    28801 ttttcgccat ctccagcgag gagtcacgtt ttactctaag cggagcttta ctccttttga
    28861 agagccaggg catgtcgatg gtttcgaccg acggacaccg cttggcgttc gttgacacga
    28921 agtccgaatt agcgggggtc aaaggatcct atcgcgcgct gttgccgaag aaggccatgg
    28981 gcgagattct taaactggct caggagtcgg ctccggacgc gagcgtgcaa tttgccggcg
    29041 acgagaacca cctgttcttc cgctttggcg aacggcttct gatcagccgc aagctgaccg
    29101 gcaatttccc cgacttcgag cgcgttcttc cccgtgaaca gccgctctct gtgattctcg
    29161 ggcgggaaga agcaagggcc gcaatcgaaa gagttgccca gttttctgac gagcgctctc
    29221 acgcggtcag aatccaggtt tgtcctggcg aggtaaaggt acattcttcg atttctgaaa
    29281 ctggcgagag tgaagaaagc atccccaccg aatacgacgg agccaccgtc gagatcggat
    29341 tcaatgcaca gtacatgctc gacttcctga gggcgtctga agaggacaaa gtggtgcttc
    29401 acttcaagga cgggcacagc gccggtgagt tgaggcccgc cgatgacgct ggaaaatacg
    29461 actaccgcta cgtcgtgatg cctatgcgga tttgagcctg tttccgggca tggaaagccc
    29521 ggcaattcac ggagagggtg ctggctgggc agtcccacaa ggagtattaa gggtcaacta
    29581 tgggttccaa ggatgtagaa ctgaacaccg tcgacacgta cgattccgca agtattaaag
    29641 tcctcgaagg tctcgaggct gtgcgcctgc ggccggcgat gtatatcggt tcgacgggcg
    29701 agaccgggct tcaccacctt gtctacgaag tcgtggataa ctcggtggac gaggcgctgg
    29761 ccggcttttg cgacgaaatc aacgtcacaa tccacttcga taattccgta actgtcgtgg
    29821 acaacggccg tggcatcccg gtggatatcc atgtggagga aggcatatcg gccgcgaccg
    29881 tcgtgatgac gaagctgcac gccggcggca agttcgattc aaagtcctac aaggtctccg
    29941 gcggcctgca tggggtcggt gtgagctgcg tcaacgccct ttccgaacga ctggagctcg
    30001 agatttggcg ccagggcttt acctggacac aggagttcga gcggggcaat cccgttgccc
    30061 ccctgcagag agccggcaag gccggtcgca agaccggtac aaaagtaact ttcaagcccg
    30121 accccacgat catggtcgtc acggagttca atttcgacat cctgtcgcag cggctccggg
    30181 aaatcgcctt cctgaataag ggcctgcgca tcaccatgac ggacgagcgg gtcgatccgc
    30241 cgaagtctca cgagttccaa tacaacggag gcatcgccga gttcatcaag cacctcaacc
    30301 gaggcaagac cgtccttcac gacaagccga tcgcttttga gggggagcgt gagttgccca
    30361 acggaggcac gctcaccatg gacgtggcgc tccagtacaa cgacagctac gcggaaaacc
    30421 tcttcagctt cgccaacaac atcaacacgg tggacggtgg ctcccacctc accggctttc
    30481 gcagcgcctt gacacggacc atcaactcct acgggcaaca ggccggactg ttcaaggacg
    30541 tcaaggaaaa tctcaccgga gacgacgttc gcgaaggcct gacggccgtc gtgagcgtga
    30601 agctcccgca accgcagttc gaaggccaga ccaagggaaa gctcaatagc gacatccagg
    30661 ggttcgtggt ccagtttgtc aatgagaagc tgagcgaata cttcgacaaa aacccttcgg
    30721 tcatgcgcaa gatcgtcggc aaggcgatcg aagcttcccg cgcacgcgag gccgcccgca
    30781 aggcccggga cctcacccgc cgcaagggcg cgctcgactc cggaggtctt cccggcaaac
    30841 ttgccgactg ccaggagcgc gatcccgagc ggtgtgagct gtttctggtc gagggcgagt
    30901 cagccggcgg caccgccaag gcgggccgcg accggcgcta ccaggccatc ctgcctctga
    30961 agggcaagat cctgaacgtc gaaaaggcgc gctacgacaa gatgctcggc catgacgaga
    31021 tccgtgccat gattaccgcc atcggcaccg gcatcggcaa agaggaaggc gacttcgacc
    31081 tggccaagct ccgctacggg aagatcatca tcatgactga cgcggatgtc gacggctcgc
    31141 acatccgtac cttgctgctg acgttcttct ttcgccacat gcaggacttg atcaatcgcg
    31201 ggcacgtttt cgtggctcag ccgccgctct accgcatcaa gaagggcaag agcgaacgct
    31261 acatcaagga cgagaaggaa ttcatgcggg agatcctgcg gcgcgccacg gagaacgtcg
    31321 tggtggaggt cagcgctcac aacggctctc cgtcacagcg gatcgaaggc acggagctga
    31381 gaaactttct catggcgctc gaggagtacc tccagatctc gtccaaggcg gagcgcaagc
    31441 tgagggacag ccgcgcggta accgttgtct ccaaccccga gctgaagatc gactccaagt
    31501 cggatttcgg cgacgagaac aaccttaagc cgttggcacg ggcgctcgag gcggccggga
    31561 tccccgccga gctgaaacac gacgaggagc attcgtcatg gatggtttcc ttccgtgatt
    31621 cgacgcacgc tgagcggacc atcggtgttg aactcgcggc catgcccgaa taccgccgcc
    31681 tgcggtcgct cggcaggcag atcggacgat acaaccagcc gccctttgtc gtgctcaagg
    31741 actcgagccg tgtgatccag gaacactggc gcggcctgct cgactacgtc aaagaggagg
    31801 gtaagaaaga cgcctccgtc cagcggtaca aggggctcgg cgagatgaac gccgagcagt
    31861 tgtgggaaac caccatgaac gcggagaagc ggacgctcct ccgggtccgc ctcgaggacg
    31921 cggtcgagtg cgaggagatc ttctcgacgc tgatgggcga ggacgtcga
//
LOCUS       DATIFQ010000437         4051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1486666, whole genome shotgun sequence.
ACCESSION   DATIFQ010000437 DATIFQ010000000
VERSION     DATIFQ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486666"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2606)
                     /locus_tag="VLE22_21915"
     CDS             complement(<1..2606)
                     /locus_tag="VLE22_21915"
                     /inference="COORDINATES: protein motif:HMM:NF023636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HSB17122.1"
                     /translation="MKISRRTLFLGAAAGAAASQTSVNRPRLERVTGRRFTGDSLREI
                     AFPLGGIGTGTVSLGGYGNLRDWEIFNRPNKGGILPFTFVALRLAGADLAKPVVRVVE
                     RRPHPPFTGGSGVPRETGLGLPRLREAVFTGAYPFANIRFQDPKLPVEVSLEAFNPMI
                     PLETDDSSLPAAVLTYRLTSRAKSNLDASLAFSILNPVGYDGVARLGSRRATFLGQNL
                     NEHKRDKGVQGLLMTSRKWPADSTRYGSMAIVTDALDVSCRPRWEHGAWWDEFQKWWD
                     EFVDRGRYSDATSEPSGDGGSEYATIAAHVPLSPGQSKAVTFVLAWHFPNTENYWTGE
                     AEYKGKALKNHYGTRWPSAWEPATYTLSNLASLRERSEHYRDTLYRSTLPPSVIDAAS
                     SQASIMRTNTVMVLEGKKTLAFEGCNDNAGCCPMNCTHVYNYEQAMAHLFPDVERSMR
                     EIDFLVNMRPDGSMGFRTKVPLQPGGTVPFPAADGQMGCVMKVYREWRLCGDDEWLRK
                     LWPEVKRALEFAWTKWDADRDGVMEGEQHNTYDIEFYGPNTMMGTFYLGALASASKMA
                     VHLDDNAAAERYRKLMEEGATRLDKLLWNGEYYVQKIDERAEKASRYQYGEGCLSDQL
                     LGQWFAEVVGLGKLLPHEHIQSTLRAIFRHNFREDFSDFPNAQRLYALNDEAGLLLCS
                     WPRGHRPALPFVYSDEVWTGIEYQVAAHLFYEGMFKEGLAIVDAVRNRYDGVRRNPWN
                     EIECGHHYARAMSSWSLVLALSGFQWSAPEKALRFSPLVNQDNFHSLYSTGTAWGSYS
                     QKRAKQKLDAEITVEGGRLELAQLRLPFPGKPSGSSLAGATVEAAGGEAIIRPKKPLR
                     LGKGDRLAVSVG"
     gene            complement(2603..3709)
                     /locus_tag="VLE22_21920"
     CDS             complement(2603..3709)
                     /locus_tag="VLE22_21920"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSB17123.1"
                     /translation="MSTYSTTVSRLPVVNSCTVTRPRLVLVGGFLGAGKTTLLLAAAA
                     RLQTAGRRVAIITNDQGGELVDTRFLRASGLKAGEVTGGCFCCRFSDFIRSAEQHLSF
                     DPDVVFAEPVGSCTDLSATILQPLKRFYGGSFLLAPLTVLVEPQRAAALLAPDSDPHF
                     SYLFRKQLAEADLVLYSKADRGADFPSIPGVTARPVSALTGDGVGEWLDEILHDGATA
                     SVRLIEVDYARYAEAEAALGWLNWQADVRLARPLTPAAVAGPLIERLDRALTDGGISI
                     AHLKVFVQAGTGYLKAGVCANGEEPSVDGALDAPPASRHELVLNLRAGASPERLDSMA
                     QDAFAAIPGQLRFLHRQSFRPSPPVPEHRFSEIV"
     gene            3708..>4051
                     /locus_tag="VLE22_21925"
     CDS             3708..>4051
                     /locus_tag="VLE22_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17124.1"
                     /translation="MDGEPLRSEDKRLIVVVLLVAVASVIFTQLNFFAAFPEASIDLR
                     YSKEQITKFAEDFLRGQGLSTTGFRRLTLFDPDDTAKTYLERELGLDQANRLMQERVP
                     VWRWRARWFRPP"
BASE COUNT          778 a         1268 c         1281 g          724 t
ORIGIN      
        1 taccccactg aaactgccag cctgtcgcct ttgcccagcc ggagcggttt cttcgggcgg
       61 atgattgcct cgccgcccgc ggcttcgacg gtcgcgccgg cgagcgatga tccggacggc
      121 ttgccaggga agggaagccg gagctgcgcc agttccaacc tgccgccctc caccgtgatc
      181 tcggcgtcca gtttctgttt cgcgcgcttc tgggaatagc tgccccaggc agttccggtg
      241 gaatacagcg agtggaagtt gtcctgattc acaagcgggc tgaagcgcag cgccttctcg
      301 ggagccgacc actggaatcc ggatagcgcc agaaccaacg accaactcga catcgcccgc
      361 gcataatggt ggccgcactc aatctcgttc caggggttgc gccggacgcc gtcgtaacgg
      421 ttccgcaccg catctacgat cgcaaggccc tccttgaaca tcccttcgta aaacaggtgt
      481 gctgcgacct gatactcgat gccggtccag acctcgtcgg agtacacgaa cggcagggct
      541 gggcggtggc cgcgaggcca actgcacagc aaaagaccgg cctcgtcgtt caacgcatag
      601 aggcgctgcg cgtttgggaa gtcggagaag tcctctcgga agttgtggcg gaagatagcg
      661 cgcagggtgg actggatatg ctcgtgcggc agcagcttgc cgaggccgac cacttccgcg
      721 aaccactggc cgagcaactg gtccgacagg cagccctccc cgtactgata cctggaggct
      781 ttttcggcgc gctcgtcgat cttctgaacg taatactcgc cgttccatag cagtttgtcc
      841 aaccgggtag cgccctcttc catcagcttc cggtaacgct cagcggcggc gttgtcgtcg
      901 agatgcaccg ccatcttcga tgcggaggca agcgcaccga ggtagaaggt gcccatcatc
      961 gtattgggcc catagaattc gatgtcgtag gtgttatgct gctcgccctc cattacgccg
     1021 tcgcggtcgg cgtcccactt cgtccaggca aattcaaggg cgcgcttcac ctcgggccag
     1081 agcttgcgca gccattcatc atcgccgcac agccgccact cgcgatacac cttcatgacg
     1141 cagcccatct gcccatcggc ggctggaaaa ggcacagtac cgcctggctg aagcggcact
     1201 ttcgtgcgga aacccatgct gccgtcggga cgcatgttga ccagaaagtc aatctcgcgc
     1261 atgctgcgtt ccacgtccgg gaacaggtgg gccatcgctt gttcgtagtt gtagacgtgg
     1321 gtacagttca tcgggcaaca gccggcgttg tcgttgcagc cttcgaaggc cagcgtcttt
     1381 ttcccttcga gcaccatcac cgtgtttgtt cgcatgatcg acgcctggct cgaagcagcg
     1441 tcgatgacgg acggtggaag ggtggaacgg tagagtgtgt cgcgatagtg ctccgagcgt
     1501 tcccgcaagg atgccaggtt cgagagcgta taggtcgccg gctcccacgc cgaaggccaa
     1561 cgagtgccgt aatggttctt cagagccttt cctttgtact ccgcctcgcc ggtccagtag
     1621 ttttcggtgt tcgggaagtg ccaggcgaga acaaaagtga ctgccttcga ctgtcccggc
     1681 gaaagcggga cgtgggcggc gatggttgcg tactcgctgc cgccgtcgcc tgagggttcc
     1741 gaagttgcgt ccgagtagcg ccccctgtcg acgaactcat cccaccattt ctgaaattcg
     1801 tcccaccatg cgccgtgttc ccagcgcggg cggcaggata cgtcgagcgc atcggtgacg
     1861 atcgccatcg acccgtatcg agtggaatcc gcaggccatt tccgggatgt catcagcagg
     1921 ccctgaacgc ctttgtcccg cttgtgctcg ttcaggttct gccctaagaa ggttgcgcgc
     1981 cggctgccga gccgggcaac accgtcgtaa ccgaccgggt tcagaatgga gaacgcaagc
     2041 gacgcatcga gattcgactt cgcccgggac gtgagccggt acgtgagcac ggcggccgga
     2101 aggctcgagt catccgtctc cagcgggatc atcgggttga acgcttcgag cgacacctcg
     2161 accggaagct tcgggtcctg gaagcggatg tttgcgaacg gatatgcgcc ggtgaagacg
     2221 gcctcacgga gccggggaag cccgagtccg gtttcgcgcg gaactcccga accgccggtg
     2281 aacggcgggt gcggacggcg ttcgacgacg cgcacgaccg gtttggcgag gtccgcaccg
     2341 gccaatcgca gcgccacaaa cgtgaacggc aggatgccgc ctttattcgg ccgattgaag
     2401 atctcccagt cgcgaagatt gccgtaaccg cccagagaca ccgttccggt gccgatgccg
     2461 cccagcggaa aggcgatctc ccggagcgag tccccggtaa aacgccggcc ggtgacgcgc
     2521 tcaagacggg gacggttcac agaggtctgc gaggcggccg ctcccgcggc ggcgccgagg
     2581 aacaacgtgc gtctgctgat tttcatacga tctccgaaaa acgatgctcg ggtacgggcg
     2641 gcgagggccg gaacgactgc cgatgaagga aacggagctg acccgggatc gccgcaaagg
     2701 catcttgcgc catcgaatct aatcgctccg gcgaagcgcc cgcgcgaagg ttcaagacaa
     2761 gctcgtgacg cgaagccggc ggagcgtcga gagcgccgtc gaccgacggc tcctcgccgt
     2821 tggcacagac gccggccttc aagtacccgg tgccggcctg aacaaagacc ttcagatgcg
     2881 cgatggaaat accgccatcc gtcagcgccc gatccagccg ctcgataagc ggtccggcga
     2941 cagccgccgg cgtcagggga cgagcaagcc gcacgtccgc ctgccagttt agccacccga
     3001 gggcggcttc ggcctcggcg tatcgagcgt agtcgacttc gatcaagcga acgctggcgg
     3061 ttgcaccgtc atgcagaatc tcatccagcc actcgccaac accgtccccg gtcagagcgc
     3121 tcacgggtcg tgccgttacg ccgggaatgg acgggaaatc cgcgccgcgg tctgccttgc
     3181 tgtacagaac taagtcggct tccgcaagct gcttcctaaa cagatacgag aagtggggat
     3241 cggagtccgg agcgagcagt gcggcggcgc gctgcggttc caccagcacg gttaacggcg
     3301 caagcaggaa gcttccccca tagaacctct tcaacggctg caggatcgtc gccgacaggt
     3361 cggtacagct tccgacaggc tcggcgaaga ccacatccgg gtcgaacgaa aggtgttgct
     3421 cggcggaccg gatgaaatca gagaagcggc agcagaagca gccgccagtg acctcgccgg
     3481 ctttgaggcc ggacgcacgc agaaaccggg tgtccaccaa ctcaccgccc tggtcgttcg
     3541 tgatgatcgc gacacggcgg cctgccgtct gaaggcgggc ggcggcagcg aggagcagag
     3601 tggtcttacc agctcctaga aagccgccca cgagtacaag tcttggccgg gtcaccgtgc
     3661 acgaatttac cacaggcaac cgcgagaccg tggttgaata ggtggacatg gacggcgaac
     3721 ccctccgctc ggaggacaaa cggctgatcg tagtagtcct gctggtggcg gttgcctcgg
     3781 tgattttcac gcagctgaac ttcttcgctg cgtttcccga agcgtcgatc gacctccgct
     3841 attcaaaaga gcagatcaca aagttcgcgg aggacttttt gcggggtcag ggcctctcga
     3901 ccacagggtt ccgccgcctg accttgtttg atcccgacga cacggcgaag acgtatctgg
     3961 agcgcgagct gggtctcgac caggcgaacc gcctgatgca ggaacgggtg ccggtatggc
     4021 ggtggcgggc gcgctggttc cgtccgccgg a
//
LOCUS       DATIFQ010000438         6712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_721738, whole genome shotgun sequence.
ACCESSION   DATIFQ010000438 DATIFQ010000000
VERSION     DATIFQ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721738"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VLE22_21930"
     CDS             <1..1486
                     /locus_tag="VLE22_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17125.1"
                     /translation="LFALLALSTAGAQTQLTVYNQNFATVKERRVLELVKGQNEVRVA
                     DITAHLEPESVVLRHLRQPDSIQILEQNYESDPLSEGLLLRKSEGKVLDFEIGQPQTG
                     EKKIIKGKILRSGYIPHASALQRYGQQYQFNQMVYSSPEGGGQPIVEVDGKIQFGLPG
                     KPIFDALDPKAFLKPTLLWRLLSAVPGKHEVEFSYLTGGMRWEANYNAVAPEKGDTFD
                     IIGWVTLENMSGKDFENANVKLMAGDVARVMAEQGNLRAYSGFEERVAAGSPAVTERA
                     FEEYHLYTLERPTTVLDREIKQVEFVRAANVPAERIYVYDGFKLDPQYRGWDYYNIRT
                     RPEYGTVSNPKVWVMLEFQNSEKSHLGMPLPKGKVKLYRRDVDARNEFIGEDQIDHTP
                     KDETVRLYTGNAFDIVGERRQTNFRLDSNGHWVDESFEIKVRNHKTEPVTVRVVEHLY
                     RWLQWEITSTSMENKKTDARTMEFRPVIAGGGEAVIRYTVHYTW"
     gene            complement(1546..3228)
                     /locus_tag="VLE22_21935"
     CDS             complement(1546..3228)
                     /locus_tag="VLE22_21935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB17126.1"
                     /translation="MLIGPIRSRTKPLLKAGRKKDSSLNDETGELRPGRLVNDPAPDD
                     LLPNQDISGNGILEEMDLALLPSAEDAEELGEFKLDAESWESTDDPIRTYLREMGNFS
                     LLTREGEIDLARRIECGRARVRKALSRVPLVIGEMLKLGEALQQGRVTVRDVLVTPET
                     ADVDDSGAKQREELLQRLSEIAKHQERAERVRQKLQDGWRGMRPKRLRKLRYDMARSL
                     VRLSRIYRQVPLRLEFERKLINLIRQAVAQYRPVEREIAKIQRRLEQSVLAGSAGQKE
                     ELRTSLQQLIERLRKLEKDWGWGALDLRRTLRIIERGEQEAETAKKRLVEANLRLVVS
                     IAKRHTGRGLQFLDLIQEGNIGLMRAADGFDYRRGYRFSTYATWWIRQGITRALADQA
                     RTIRLPVRMIEAIRRLAGAQRELRQELRRDPTPGELARKMEISVSKVRRILRAAQEPI
                     SLETPIGEEMDSSLGDLLVDHEAVSPSQSIISLNLREQTAELLGALKPREAEILKMRF
                     GLLDDRVYTLEEIGLRFAVTRERIRQIEIRALRKLRAPSRSHHLKVFLDSGS"
     gene            3398..4696
                     /locus_tag="VLE22_21940"
     CDS             3398..4696
                     /locus_tag="VLE22_21940"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HSB17127.1"
                     /translation="METSAEMANEFAFEMATSAIRFGFGVTREVGMDLAELGARRALV
                     LTDPTVRRLPPGERVLESMAKGKICCAVYDHVRIEPTDESFRDAIAFASAGHYDAFVA
                     VGGGSAIDTAKAVNLYTTYPPGDFLDYVNPPIGKGVPVPGPLKPLMAIPTTAGTGSET
                     TGVAIFDLARMHAKTGIASRRLKPTLGLLDPENTRTLPPMAAASTGLDVLTHAIESYT
                     ALPFAQRPRPELPALRPAYQGSNPISDIWSLEALRMLAKYLFRAVEDPSDDEARANML
                     LAASYAGVGFGNAGVHLAHGMSYPVSGMVRGYRADGYPADRPLIPHGISVVLNAPAVF
                     RFTAPACPERHLRAAEVLGADVSRARPDDAGKILAERITAFMYRLKVPNGLSAVGYTA
                     DDIPALVEGTLPQHRVTKLSPRPAGPEDLARLFEDALKAW"
     gene            5093..5491
                     /locus_tag="VLE22_21945"
     CDS             5093..5491
                     /locus_tag="VLE22_21945"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSB17128.1"
                     /translation="MMHPLEPRALSLLRIAAGFTFSLHGFQKLFGCFGGMGGSGATAA
                     FGTLPWVAGVLEAFGGILLLAGLFTRPVAFILCGEMAVAYFRAHAPRGFWPIANGGEL
                     AVLYCFIFLYLAVAGAGPVSLDRIVRKKSK"
     gene            5796..>6712
                     /locus_tag="VLE22_21950"
     CDS             5796..>6712
                     /locus_tag="VLE22_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17129.1"
                     /translation="MKSTRAYLVTLTTFIKSSRLAPYAILLLFIFISHVIILLTPGFF
                     NDEEWGAFDRIRENGLSSYIQHSVLTIPDSRHGFSDSLRPIGYLHLGLAAVWMQSAPF
                     VSHTMSVLVHALACILLVRVLAMLGAAPALAYLSGALFSLSPLTTTAVGWIAANMDLW
                     YIVFVLCSCILLLNVAKRGLTFRSACGIVVCSGLAILSKETALLLPGAMLLLVLVARR
                     ASPRNHIHLKDSLLGVSVSIVPMCVFLAIRMPALAVSLSGNGHPAYRPELANVARNAA
                     YYVSYPFLYNLHELINVVFVGSGSLILAL"
BASE COUNT         1307 a         2003 c         1973 g         1429 t
ORIGIN      
        1 gttgtttgca ttgcttgcgc taagcacggc gggtgcgcaa acccagctca ccgtctacaa
       61 ccagaatttc gccacggtaa aggagaggcg tgtcctcgaa ctggtgaaag gccagaacga
      121 ggtccgcgtc gccgatatca ccgcccatct ggagcccgag tcggtcgtgc tccgccatct
      181 tcgccagccg gattcaatac agatactgga gcagaactac gagagcgatc cactgagtga
      241 aggtctcttg ttgcgcaagt cggaaggcaa ggtgctggat ttcgaaatcg gccagccaca
      301 gacgggcgag aagaagatca tcaaagggaa gattctgcgg agcggctaca tcccccacgc
      361 ctccgcgctt cagcgctacg gccagcagta tcagttcaac cagatggtct actcgagccc
      421 ggagggcggc gggcagccga tcgtggaagt agatggcaag atccaattcg gtctgcccgg
      481 caagccgatc ttcgatgcac ttgatccgaa agcgttcttg aaaccaacac tgctgtggag
      541 gcttctctct gccgtcccgg gcaagcatga agtcgagttc tcctatctca caggcgggat
      601 gcgttgggag gcgaattaca acgccgttgc gcctgaaaaa ggagacacct tcgatatcat
      661 cggctgggtg actctcgaga acatgagcgg caaggacttc gagaacgcca acgtgaagct
      721 gatggccgga gatgtcgctc gagtcatggc ggaacaagga aatctcaggg cctactcggg
      781 attcgaggaa agggtggccg caggcagtcc tgccgtaacc gagcgcgcgt tcgaggagta
      841 ccatctgtac acgttggagc ggccgacgac ggtactggac cgggaaatca aacaagtgga
      901 gtttgttcgc gccgccaacg ttccggccga aagaatttac gtatacgacg gttttaagct
      961 ggacccacag tatcgcggct gggattatta caacatccga acccggcctg aatatgggac
     1021 cgtgtctaac ccgaaggtat gggtgatgct ggagtttcag aattccgaga aatcgcacct
     1081 cggcatgcca ctccccaagg gcaaggttaa gctctatcgg cgggacgtcg acgcacggaa
     1141 cgagttcatc ggagaagacc agattgacca cacgccgaaa gacgagaccg tgcggcttta
     1201 caccggcaac gcattcgaca ttgtggggga gcgccgccaa accaatttca gactggactc
     1261 aaacggccat tgggtcgatg agtccttcga aattaaggtg cgcaaccaca agacggaacc
     1321 ggtcactgtg cgcgtagtgg agcatctgta ccgctggcta cagtgggaga ttacgtcaac
     1381 ttcgatggag aacaagaaga cagacgcgcg cacgatggag ttccggccgg tgatagccgg
     1441 cggtggcgag gcggtcatcc ggtacacggt tcactacact tggtgaagga gatcacacgg
     1501 tccggccatt tctcaccggt acgcactttc gcctttagat cagcctcacg agccgctatc
     1561 gagaaaaact ttcaggtgat ggctgcggct gggggctcgg agcttgcgga gcgccctgat
     1621 ctcgatctgc cggattctct ctcgtgtcac cgcaaagcgc aggccaatct cctcgagggt
     1681 gtagacgcgg tcgtccaata atccgaagcg catcttaaga atctctgcct ctctcggttt
     1741 gagcgccccg agaagctcgg ccgtttgctc tcgaaggttc aggctgatga tcgactggga
     1801 gggcgagacg gcttcgtggt ccacgagaag atcgccgaga gacgagtcca tctcctcccc
     1861 gatgggcgtc tccagcgaga tcggttcctg tgcggcccgg agaatcctcc gcactttgct
     1921 cactgagatt tccatttttc gggctagttc cccaggcgtc ggatcccggc gcagttcctg
     1981 ccggagttcg cgctgggcgc ccgccagcct tctgatagct tcgatcatgc ggaccggaag
     2041 ccgaatggtc cgcgcctgat cggcaagggc gcgcgtgatc ccctggcgga tccaccaggt
     2101 cgcataggta gagaacctgt acccgcgccg gtagtcgaat ccgtcggcag ccctcatcaa
     2161 gccgatgttg ccttcctgga tcaggtccaa aaactgcagg ccgcggccag tgtggcgctt
     2221 ggcgatcgac accaccagcc gcaggtttgc ctcgacgagc cgcttctttg ccgtctcggc
     2281 ttcctgctcg ccgcgctcga tgatccgcag cgtgcgccga aggtccaacg cgccccatcc
     2341 ccaatccttc tccagcttcc gcaaacgctc aatcagttgc tgcaacgacg tgcgcagttc
     2401 ctctttctgc ccggctgagc cggctagcac actttgctcc aacctccgct gtatcttcgc
     2461 aatctctcgt tccaccggtc tgtactgcgc gaccgcctgc ctgatcaggt tgatgagctt
     2521 acgctcgaac tccagtctga gtggaacctg acggtagatc cgagacagcc tcaccaaaga
     2581 acgagccatg tcgtaccgca gctttctgag ccgtttcggt ctcatgccgc gccagccatc
     2641 ctgaagcttc tggcggaccc gctcggccct ttcctggtgc ttggcaatct cggagagccg
     2701 ctggaggagt tcttctctct gcttcgcgcc ggaatcatcg acgtccgccg tctccggcgt
     2761 gaccagcaca tcccgcacgg tgacccgccc ttgctgtagc gcctcgccca gcttgagcat
     2821 ctctccaatc accaggggaa cccgcgacaa ggccttcctc acccgtgcac ggccgcattc
     2881 gatgcgcctg gcaagatcaa tctcaccctc ccgggtaagc agggagaagt tccccatctc
     2941 gcggaggtat gtgcggatgg ggtcatcggt actttcccaa gactcggcat cgagtttgaa
     3001 ctcgcccagt tcttcggcgt cctcggcgct tgggagcagt gcaagatcca tctcctcaag
     3061 aatcccgttg ccggaaatat cctggttcgg gagcagatcg tcgggggcgg ggtcattcac
     3121 cagccggccc gggcgcaatt caccagtctc gtcgttcaga gacgagtctt tcttcctgcc
     3181 cgccttcagg agcggtttgg tacggctgcg tattgggccg atcagcaaag ctgtcccctc
     3241 tggcgcctgg atcacaaaac gactcaagtc aggctacctg aaacctctgt catcgcattc
     3301 ccgagtaacc gttatatatc acaagggcgg agagttgtcc atggaatata caggaaaggc
     3361 ggctgggttt atactggctc agacgctggg tagcaaaatg gaaacttctg ctgagatggc
     3421 aaacgaattc gccttcgaaa tggcgacttc cgcgatccgg ttcggcttcg gcgtgacacg
     3481 cgaagtgggc atggatctgg ccgaacttgg cgcccgccgc gccctcgtcc tgacggatcc
     3541 caccgtacgc aggctgcccc ctggggaacg cgtgctcgag tcgatggcca aagggaagat
     3601 ctgctgcgcg gtgtacgatc acgtacggat cgagcccacc gacgaatcgt tccgcgatgc
     3661 catcgccttt gcgtccgcgg gccactacga cgccttcgtg gccgtgggcg gcggttcggc
     3721 gattgacaca gctaaggcgg tgaacctgta caccacgtat ccgcccggcg atttcctcga
     3781 ttacgtaaat ccgcccatcg gaaaaggcgt gccggttccc ggtccgttga agccgctcat
     3841 ggccatcccg actaccgcgg gcacgggctc cgagaccacg ggagtggcta tcttcgatct
     3901 ggctcggatg cacgccaaga cggggatcgc cagccgccgg ctgaagccca cgctgggcct
     3961 gctcgatccc gagaacacgc gtactctgcc gcccatggcc gccgcatcga ccgggctaga
     4021 cgtgctcact cacgccatcg aatcctacac cgccttgccg ttcgcgcagc gcccgcggcc
     4081 ggagcttccc gccctgcgcc ccgcctatca gggttcgaac ccgatcagcg acatttggtc
     4141 cctggaggcg cttcggatgc tagcgaagta cctgtttcgc gcggtcgagg acccgtccga
     4201 cgacgaggca agggcgaaca tgctgctggc ggcctcgtat gccggagtgg ggtttgggaa
     4261 cgcgggcgtc cacctggcgc acggcatgtc atatcccgtt tcgggcatgg tgcgcggtta
     4321 tcgcgccgac gggtacccgg cggaccggcc gctcataccg cacggcatct cggtcgtgtt
     4381 gaacgcgccc gctgtgttcc ggttcacggc gccggcttgc cccgagcgtc accttcgggc
     4441 cgccgaggtg ctgggagcgg atgtctcccg cgcacggccg gatgacgccg ggaagatcct
     4501 cgcggagcgg atcacggcat tcatgtaccg gctgaaagtc ccgaacggac tcagtgcggt
     4561 cggctacacg gccgatgaca tccccgcgct ggtcgagggt accttgccgc aacaccgcgt
     4621 caccaagctc tcgccgcgcc cggccggccc cgaggacctg gccaggctgt tcgaggatgc
     4681 gctgaaggcc tggtagagtc ttgaggagtt tgctgtgtct cttggaacac tctgaggcag
     4741 gcgtttacgt tataatccta cgagtttcag cttcgcgtca gaggaggatt tatgcctaga
     4801 ggaagaagac ggtcgataga accgaaggtt cttgaagctg cacttgttgg cctggaatca
     4861 cggcgagagc gacttgatca gcagattgcg gaagtccggc ggctgatcgg cgggagagga
     4921 actcgcaagg cggctgcggc gccggcagca cccgctcgca agaggcgtaa actcagcgca
     4981 gcagcgctga agcgcattgc agatgcgcaa cggaagcggt gggcggagtg gcggaagaaa
     5041 gggaaggccg cgggcgcaaa ggcgaaagga tagtccgcca gggggcacgg ccatgatgca
     5101 cccactggaa ccgagggcac tttcgctcct acggatcgct gcgggattca cgttctcact
     5161 ccatggtttt cagaaactct tcggctgctt cggcggaatg ggtggaagcg gcgctacggc
     5221 cgcgtttgga accctgccgt gggtggccgg agttcttgaa gcatttggcg ggatccttct
     5281 tctggccgga ctcttcaccc ggcctgtggc ttttatcctt tgtggcgaga tggcggtggc
     5341 gtacttccgg gcgcatgcgc cgaggggctt ctggccgatt gcgaatggcg gcgagcttgc
     5401 cgtgctctac tgcttcattt tcctgtacct tgcggtcgcc ggcgcgggcc cggtaagcct
     5461 cgatcgaatc gttcgcaaga agtcgaagtg atgcttggtg cggggcccat ttccagggct
     5521 tggagaggaa gacgggactc gaacccacga cgtccagttt ggaaagcgtc ctggctaaag
     5581 gctgagcgac aacgtcggct acgaggacat ccgcgcgttc cagagctgcg agatctcact
     5641 catgcccaac cccgaacatc gattcaattc cagcgacagc ctgactctga cccagggacg
     5701 ctgatcgcct cgctgcggac ggggaaccat catctgcggc tgttcaggaa gtctgtatca
     5761 tctcttaatc tgtcgctgcc ggcacatatg ctgaaatgaa atccactcgc gcttatttag
     5821 tcacgctcac caccttcatc aaatctagca ggctagctcc ttatgcgatt ctcctgctct
     5881 ttatctttat tagtcatgta ataattctat tgacacccgg cttcttcaac gacgaggaat
     5941 ggggggcctt tgaccgcatt cgtgaaaatg gtctatcgtc ttatattcag cacagcgtat
     6001 tgactatccc cgattcccga catggcttta gtgacagcct gagacctatc ggatacctgc
     6061 atcttggctt ggctgctgtc tggatgcagt ccgcgccttt cgtatctcac acgatgagtg
     6121 tccttgttca cgccctcgcg tgtattttac ttgttcgagt tcttgctatg ctgggagccg
     6181 ctcctgcact tgcatacctc agcggtgctc tcttttcgtt gtctcctctc actacaacag
     6241 cagtcggctg gattgcggcg aatatggatc tatggtacat cgtttttgtt ctgtgctcct
     6301 gcatattgct attgaacgtg gcgaagaggg gcttaacgtt cagatcggca tgtggcattg
     6361 tcgtctgcag tggcctcgct atcctctcca aggaaacggc cttgcttttg ccaggcgcca
     6421 tgctcttgtt ggtcttggtc gcgcgccgtg cctcgccacg taatcacata cacctaaaag
     6481 actctctact aggcgtttcg gtatctatcg tgccgatgtg tgtctttctc gcaatccgca
     6541 tgccagctct tgctgtatcg ctgtccggga acggtcatcc tgcataccgg cccgaactgg
     6601 caaatgtcgc aaggaacgct gcctattatg tttcgtatcc atttctgtat aatcttcatg
     6661 aattaataaa cgtggtgttt gttggttcgg gcagtttaat actggcatta at
//
LOCUS       DATIFQ010000439         3442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1296761, whole genome shotgun sequence.
ACCESSION   DATIFQ010000439 DATIFQ010000000
VERSION     DATIFQ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296761"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..947)
                     /locus_tag="VLE22_21955"
     CDS             complement(336..947)
                     /locus_tag="VLE22_21955"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSB17130.1"
                     /translation="MLDRYEAVLFDFDGVLVDSEPVHFECWAEVFRPFGIHLDWETNG
                     KRCVGWSDRATVEYFASLANPPVPFERLWAEYPRKSALFVKRLMENPPWADGIFDLFK
                     SLQNYKLSVVTSSVRSEIEPILDAGGLSAIFDTVVYGEDVEHHKPEPDPYLLAASRLH
                     TKSALVVEDSDAGERSGRAAGFDVLRIPHASQTTRLVRERLGL"
     gene            complement(947..2305)
                     /locus_tag="VLE22_21960"
     CDS             complement(947..2305)
                     /locus_tag="VLE22_21960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSB17131.1"
                     /translation="MTHTRRPMALFCLLAFLAVPVLGAQRKLKPGFNLFSKDQDVQLG
                     KEAAAEVEKQYEVVTDPVLDGYLKRIGNKLASQPEADKYPYTFKLVKDPTLNAFALPG
                     GPTFVHTGLITAAENEAQLAGVMAHEIAHVALRHGTNQASKANLLQLPAMLAGAAVGS
                     SMVGQLAQLGIGLGANSVLLKFSRNAERDADLLGARMMAGAGYNPIEMARFFEKLEAD
                     GGSRGPQFFASHPNPGNRVKAVEEEIRYLPRRDYTTGSGDFQKVKSLVAKLPPPVKKP
                     GQAATPTGGAPDVQASGQFKEFQGREFTISHPDNWRSAEGREAGTAIIAPEGGAFQDA
                     NGGVQVGLGLMTGIYTPKNARNLQEATAEFIEQVRSGNPQLQVGQGRPEQLRVDGSEA
                     IRTTLVGPSPYQNEREVDNLVTIARSNGRILYLVFVSPESKNRNAEPVFKQMLRSVRL
                     PR"
     gene            2435..3373
                     /locus_tag="VLE22_21965"
     CDS             2435..3373
                     /locus_tag="VLE22_21965"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HSB17132.1"
                     /translation="MLTPTEKIWHNGRFIDWDDAKIHVLAHVVNYGTSVFEGIRCYST
                     PAGPAIFRLREHIRRMIDSAKIYRMDLGYSLDELAEAMVELVRLNRMQACYLRPIALR
                     GYGEIGVNPGKNPIEVYVACWEWGKYLGEEALAQGVDVCVSSWTRLAPNTLPTLAKSG
                     ANYMNSQLIKMEALANGYTEGIALDSGGYVSEGSGENIFVVRDGKILTPPLGASVLPG
                     ITRDSVLKLAAELGIPVVETLVPREMLYIADEVFFSGTAAEITPIRSIDKITIGKGRR
                     GPVAEKLQQEFFAIIEGSKPDRYGWLTPVNVTVGVK"
BASE COUNT          649 a          991 c         1140 g          662 t
ORIGIN      
        1 aggcgatcga ggtcgctcgt tccctgcccg atgctggcgt tcatctgccc gccatggtgc
       61 agaatcgtgc gcatatcgcg caggcgaaag atgcgcctcc tggaggggtg ccggcgaatg
      121 gtgatggccg gtggtttgat accgatcgtt tcgaaaggac cgatgggacg ctccaaagga
      181 ctgatgggac gcgactcggc aatcggcctc cgggcgcgct cgtagcggcg ccaatcggtc
      241 gaatccgtag cggaacggcg accgcgccgg cgaactcaaa gaacgaagcc ggtcgctgac
      301 caccggtgga agccggtgcc acctggtcgg agttcttaca gcccaagccg ctcgcggacg
      361 aggcgggtgg tctgggaagc gtgggggatg cggaggacgt cgaagcccgc ggctcggccg
      421 gatcgttcgc cggcgtcaga atcctcaact accagtgcag attttgtgtg tagtcgactg
      481 gcggccaaaa ggtaagggtc cggctcaggc ttgtggtgtt cgacgtcctc gccatagacg
      541 acggtgtcaa aaatagcaga tagaccgccg gcgtccagaa tcggctcaat ttccgatctg
      601 acacttgagg ttacgactga taatttatag ttttgcaatg acttaaataa atcgaagatg
      661 ccgtcggccc aaggcgggtt ctccatgagc cgcttcacga acagggcgct ctttcggggg
      721 tactcggccc agaggcgctc gaacgggacc ggcgggttgg cgaggctggc gaaatactcg
      781 acggtggcgc gatcgctcca tccgacgcaa cgctttccat tcgtctccca atccaagtga
      841 attccaaagg gtcggaagac ctcggcccag cactcaaaat gaaccggctc actatcgacc
      901 agaacaccgt caaagtcgaa aagaacggct tcgtagcggt ccagcattac cgcggaagcc
      961 tcacggagcg aagcatctgt ttgaagaccg gctccgcgtt ccgatttttg ctttcgggcg
     1021 agacgaagac aagatagagt atgcgtccat tggaccgggc aatcgtcacg aggttatcga
     1081 cttcccgctc gttctgatat ggggacggcc cgaccagcgt ggtccgaatg gcttcgcttc
     1141 cgtcgactcg gagctgttcc ggtcttccct gcccgacctg aagctgcggg ttgccggagc
     1201 gaacctgctc gatgaactcg gcggtcgctt cctggaggtt acgagcgttc ttgggcgtat
     1261 agatcccggt cataagaccg agaccgacct ggacgccgcc gttggcgtcc tggaacgcgc
     1321 cgccttcagg ggcgatgatg gccgttcctg cctcgcggcc ttcggcggag cgccagttgt
     1381 cggggtggga gatggtgaat tcccttccct gaaactcctt gaattggccg gaagcctgga
     1441 cgtccggggc gccgccggtg ggggtcgcag cctgcccggg ctttttcacg ggtggtggca
     1501 gcttggcaac gagcgacttc actttctgga aatcgccgga gccggtggtg taatcccggc
     1561 gcggcaggta ccggatctcc tcctcgacgg ccttcacgcg gttaccgggg ttggggtggc
     1621 tggcgaagaa ctgcgggccg cgggaccccc cgtcggcctc gagtttttcg aagaagcggg
     1681 ccatctcgat ggggttgtag ccggcgccgg ccatcatgcg agcgccgaga aggtcggcgt
     1741 cacgctcggc gttgcgggag aacttcagga gcacggaatt ggcgccgagc ccgatcccga
     1801 gctgggcgag ctgacctacc atggagctgc cgacagccgc gcccgcgagc attgccggca
     1861 actgaagcag attcgctttg ctggcctggt tggtgccgtg gcggagcgcg acatgcgcaa
     1921 tttcgtgcgc catgacgccc gcgagttgtg cttcgttctc ggcagcggtg atcagcccgg
     1981 tatggacgaa ggttgggccg ccgggcagcg cgaacgcgtt cagggtcggg tccttgacca
     2041 gcttgaatgt gtaggggtac ttatcggcct cgggctgaga agcgagtttg ttgccaatgc
     2101 gcttcaggta accatcgagc accgggtcgg taacgacctc gtactgcttc tcgacctcgg
     2161 cggcggcttc tttgccgagc tggacgtcct gatccttgga aaacaggttg aaaccgggct
     2221 tcagcttgcg ctgcgcgcca agaacgggca ctgccaggaa tgccagcaaa caaaacagag
     2281 ccatcggccg tctggtgtgt gtcatctcct ccgctcctcg gttctcatct ccgggaaaac
     2341 cggggataga ctgcgccgtc cgcgcgcaaa agacgcttgg gacggtgaag tcagtccccg
     2401 gttttccgtc tagtgaccac gatataatca tggcatgctg acgcccaccg agaaaatatg
     2461 gcacaacggc cgtttcattg attgggacga cgcgaagatt cacgtcctcg ctcatgtcgt
     2521 caactacggg acgtcggtgt tcgagggaat ccgctgttac tcgactccgg cgggacctgc
     2581 gattttccgc cttcgcgagc acattcgccg catgatcgat tcggcgaaga tctaccggat
     2641 ggatctcggc tacagcctcg atgagctcgc cgaggcgatg gtggagctgg tacggctgaa
     2701 ccggatgcag gcgtgctacc tgcgcccgat cgcgcttcga gggtacggcg agatcggcgt
     2761 gaacccgggc aagaatccga tcgaagtcta tgtcgcctgc tgggagtggg gaaagtatct
     2821 cggcgaggag gcgctggcgc agggagtgga cgtgtgcgtc tcgagctgga cccggctggc
     2881 gccgaatacg ctgcctacgc ttgccaagtc gggcgcgaac tacatgaatt cgcaactcat
     2941 caaaatggag gcgcttgcca acgggtatac ggaaggaatt gcgctggact cgggcgggta
     3001 cgtaagcgaa ggttcgggcg agaacatctt cgtggtgcgc gacgggaaga tcctgacgcc
     3061 gccgcttggc gcctcggttc tgccgggcat cacacgcgac tccgtgctga agctggcggc
     3121 agagcttgga attccagtgg ttgagacgct cgtaccgcgg gaaatgctct acatcgccga
     3181 cgaggtgttc ttctctggca cggcagcgga gatcacgccg atccggtcga tcgacaagat
     3241 cacgatcggc aagggccggc gcggaccggt ggcggagaag ctccaacagg aatttttcgc
     3301 gatcatcgaa ggctcgaaac cggaccggta cggctggctg actccggtga acgttacggt
     3361 gggcgtgaag tagggtgaaa acgacttgga gcgcggcccg ggcggccacc tcccccgaga
     3421 tcatccgtcc ggatcgcgct cc
//
LOCUS       DATIFQ010000440         3062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_145074, whole genome shotgun sequence.
ACCESSION   DATIFQ010000440 DATIFQ010000000
VERSION     DATIFQ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145074"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3062
                     /locus_tag="VLE22_21970"
     CDS             <1..>3062
                     /locus_tag="VLE22_21970"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSB17133.1"
                     /translation="DVISQIKIGHAGHAYVVDSSGNLIAHPDISLVLQKTNLSSLPQV
                     RDALAGPTERGRSAVEAAIAQNLQGKSVLSAYAPIVPLGWLVFVEQPLADAFAPLYAS
                     VYRTVLLLGIGIGISVLASVFLARRMVWPIQTLQAGAARIGAGDLGHRLAVRTGDEVE
                     ALATQFNCMTSQLQESYANLEQKVEERTRDLTEALEQQTATSEILRIISSSPADLQPV
                     LDAVAEKAARLCDAEDATIFRVEAGALRKVAGRGPIPIFPVGGDETVPISRGSVAGRA
                     ILDRQPVHLQDLAAADEAEFPEGRAYQRRFGNRTTLGVPLLREGTALGAILIRRMEVR
                     PFSEKQIALLQTFADQAAIAFENVRLFVELQKRNRDLIEALEQQTATSEVLKVISRST
                     FDLQPVLDTLLENAARLCGAERGQINRLEGEVYRVAAVYNIPQSLKEYLEGHPMCAGR
                     GTITGRAALERRAVQILDVLADPEYDYGEGQKLGGYRTLLGVPMLREGLPIGVIVLWR
                     EEVDAFTGRQIELVETFADQAVIAIENVRLFNEIQTRNRELTEALEQQTATSEILRII
                     SSSPTNLQPVLDAVAEKAARLCDAEDAVLFRLEGEVLRNAAGMGPIPKFEAESEEGIA
                     VNRGSVTGRAVVDRQAVHVQDLTEADPDEFPEGRAFARRFGHRATLAAPLLREGVPIG
                     AIMVRRKEAQPFSDKQIEILQTFADQAVIAIENVRLFNEIQARTRELEQSLEEVHALS
                     DVSRAVSSSLDLGHVLHEISEQAAKLCDADASFIQEYAQEAKTFHISASWNAAEDFVR
                     SIQAAQITLGKGAAGRSAVTGKPTQIPDILTEPDYPFRDILAGEGYRAVLSVPMLRDE
                     RIIGTVVVVRKAAGDFSERHVNLLTTFAGQTTIAIEHARLYRDVTEKGRLLEEANRHK
                     SAFLANMSHELRTPMNAIIGFSEVLLDPSMQVSDEERAQFLTDILHSGKHLLNLINEV
                     LDLSKIEAGRMELHVAPAALSEVFETVQSTLRPLAAKKAIELHVEPAG"
BASE COUNT          613 a          939 c          978 g          532 t
ORIGIN      
        1 ggacgtgatc tcgcagatca agatcggcca tgcggggcac gcctacgtcg tggattccag
       61 cggcaatctc attgctcacc cggacatcag cttggtgctg cagaagacca acctgtcatc
      121 gcttcctcag gtccgggatg cgctcgcggg accaacggaa cgaggccgaa gcgcggtgga
      181 ggcggcgatt gcccagaatc tccaggggaa gagcgtcctt tccgcctatg ccccaatcgt
      241 tccacttggc tggctggttt tcgtcgagca gcccctggct gacgcctttg cacccctcta
      301 cgcgtcggtc taccgcacgg tcctcttgct cggcatcggt atcgggatct ccgtgctggc
      361 tagcgtgttc ctggcccgga gaatggtgtg gcctatacag acgctgcaag ccggagcagc
      421 ccgaatcggc gcaggagacc tcggccatcg ccttgcggtg cggaccgggg atgaggtgga
      481 ggccttggcg actcagttca actgcatgac gtcgcaactc caggaatcct acgcgaactt
      541 ggagcaaaag gtcgaggagc gcacacggga tttgaccgag gcgctcgagc agcagacggc
      601 cactagcgag atcctccgga tcatcagcag ttcgccggcg gacctccagc ctgtgctcga
      661 cgccgttgcg gagaaggctg cgaggctctg cgacgccgag gacgctacga tttttcgcgt
      721 ggaagcgggt gcgctccgca aggtggcagg acgcggtccg atcccgattt ttcccgttgg
      781 aggagacgag actgttccca tcagccgcgg gtcggtggct ggccgggcca ttttggatcg
      841 gcaaccggtc catctacagg acctcgctgc agcggacgaa gcggaatttc cggaggggag
      901 agcctaccag aggcgcttcg ggaacagaac gaccctgggc gtgccgctct tacgtgaggg
      961 gaccgctctc ggggctattc tgatccgccg gatggaggtc cgtccgttct ccgagaagca
     1021 gattgcactg ctccagacct tcgccgacca ggccgccatc gcttttgaga acgtcaggct
     1081 gtttgtggaa ttgcagaagc gaaatcggga cctgatcgag gcgctcgagc aacagacggc
     1141 aacgagtgaa gtgctgaaag tcatcagtcg ctccacgttc gaccttcaac cggtcctcga
     1201 cacgctgctg gaaaatgcgg ctcggctttg cggcgccgag agagggcaaa tcaatcgact
     1261 cgaaggcgag gtgtaccgcg tggctgcggt gtacaatatt ccgcagagtc tcaaggaata
     1321 cctcgaaggg cacccgatgt gtgccggtcg gggcaccatc acggggcgtg cggcgctcga
     1381 gcgacgagca gttcaaattc tggatgtcct ggcggacccg gaatacgact acggcgaagg
     1441 ccagaaactg ggtggctatc gcacgctgct gggcgtcccc atgctacgcg aggggcttcc
     1501 catcggcgtc atcgtcctct ggcgggaaga agtcgacgca ttcacgggcc ggcagatcga
     1561 attggtcgag accttcgccg accaagcggt catcgccatc gagaacgtcc ggctcttcaa
     1621 cgagatccag acacgaaacc gggagctcac cgaggccttg gagcagcaaa cggccaccag
     1681 cgagatcctc cggataatca gcagttcccc gacaaacctt caacccgtat tggatgctgt
     1741 cgccgagaag gccgcacgcc tgtgcgatgc ggaagatgcc gtgctttttc gtctggaggg
     1801 ggaggtgctg cggaacgcgg cgggcatggg cccgattccg aaatttgagg ccgagagcga
     1861 agaaggaatc gctgtcaatc gtggttcggt caccggtcgg gcagtggtgg atcggcaagc
     1921 agtccacgtg caagacctga ccgaggctga cccggacgag tttcccgaag gcagagcctt
     1981 cgcaagacgg ttcggccacc gcgcgacgct cgccgcgccg ctcctgcgcg aaggggtccc
     2041 tatcggggcg atcatggtgc gtcgcaagga agcccaaccc ttctctgaca agcagatcga
     2101 gatcctccag accttcgccg accaggccgt gatcgccatc gagaacgtcc gtctattcaa
     2161 cgaaattcaa gcgcggacgc gcgagctgga gcaatcgctg gaggaggtgc atgcactcag
     2221 cgacgtgagc cgcgcggtca gctcgtcgct cgacctggga catgtccttc acgagatctc
     2281 agagcaggcc gcgaaacttt gcgatgcgga cgccagcttc atccaggaat acgcccaaga
     2341 ggccaagacc ttccatatca gtgccagctg gaatgcggcc gaggatttcg tgcgctccat
     2401 ccaggccgct cagatcaccc ttggaaaggg tgctgccgga cgcagcgcgg tcacgggcaa
     2461 accgacgcag attcccgaca tcctgaccga acctgattat ccgttccggg acatcctggc
     2521 cggggagggg taccgcgcgg tgctctcggt gccgatgctc cgcgatgagc ggatcatcgg
     2581 aacggtagtc gtcgtccgca aggcggcagg cgacttcagc gagcgccatg tcaatctgtt
     2641 gacgacattc gccggccaaa cgaccatcgc catcgagcac gcccggctct accgcgacgt
     2701 gactgagaag ggacggctgc tggaagaggc aaatcgccac aagtccgctt tcctggcgaa
     2761 catgtctcac gagctccgta cgcccatgaa cgccatcatc gggttctcgg aggtcctgct
     2821 cgacccgtcc atgcaggtca gcgacgagga acgcgctcaa ttcctgaccg atatactcca
     2881 cagcggaaaa cacctgctca atctgatcaa cgaggtgctc gacctgtcga agatcgaggc
     2941 cggccggatg gagctgcacg tcgcgccggc ggcgctgagc gaggtgttcg agaccgtcca
     3001 gagcacgctg cggcccctag ccgcgaagaa ggccatcgag ttgcacgtgg agcccgccgg
     3061 cg
//
LOCUS       DATIFQ010000441         6675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1677608, whole genome shotgun sequence.
ACCESSION   DATIFQ010000441 DATIFQ010000000
VERSION     DATIFQ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677608"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VLE22_21975"
     CDS             complement(<1..986)
                     /locus_tag="VLE22_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17134.1"
                     /translation="MGRPYSLHTVTVLAILLPCALIGVIGYKWIELEREAGARRGEQA
                     AEAETARIRKGLVAHLAAVTSEVARGWSGLPARRPPFEPSPPLPEIVASAFVFTPAGK
                     LAVPDYQHAYLLASRDSESTALLNQGKLSLAAGHYREAEASGQKLLGCCAARRDEYGV
                     SFALYAAAQIAAALEAQGRLRAGFPGLAAQLVDLLQRGAIGHPNDLQDIAALAARAGG
                     VPEASRLLQQAEEQAARVRNQIELASRLEKWVSGTVLAGNPGSGFTVHSLPMGDRPRL
                     AGVHRTGDSMLIALFAAGPLAAWVASEGTRGGHFDAVLAPKGEYSGNAILRDV"
     gene            1146..3260
                     /locus_tag="VLE22_21980"
     CDS             1146..3260
                     /locus_tag="VLE22_21980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17135.1"
                     /translation="MHHRKGPHTLDRREFLTVTALAGSALLPAAGCKKKIKGLVLTPS
                     GMLGGLRQDGTDTPIDDATWYVAEKPGDGFRFTFPAGTLAKAQYVTTDMLLDGNNMIV
                     FQLALQEGEDGRKFTFRFSGLNQCSFRVRMPLSLVDQNRWGIDREGAFLKPRAGGSRV
                     DLAKVDRMTVTVTRKAPKPARFALTNFIATEEEPSRIDEPVLPKGSLIDELGQSTLHD
                     WPAKSRGVDEVVQRIRKQLEEAPARKWPDTFSQWGGWKAKKLRKGTGFFTTHYDGKRW
                     WLVDPEGYAFWSAGLDCVRVDTSANYGGLETALKWLPDPKSEFGDIYEHNTDLRRRSN
                     FISYLAANMIRALGRDGWHDKWAQLVYGELRRLRFNTVGNWSEWEFARKEQFPYVRPM
                     SFQPNRVQFVYRDFPDVYHPEFEKDAADYASVLKDTADDPAFLGYFLMNEPTWGFSSE
                     VPAAGMLYVTETCGTRKELAASLKNKYPDDGALAKAWGMDVSFDKVAAGKWTAVLTPE
                     AQKDLEAFSTLMVERYFRIISSACKTVDPNHLNLGMRWAGLPPSWAVAGMKFFDVYSI
                     NCYRERVPAETTKQIQDMLKKPTIIGEWHFGALDVGLPSSGIGHLKNQVDRAKAYRVY
                     IEDAAANPNCVGAHWFILYDQSALGRFDGENYNIGFFDVCNRPYEELGAGAIASHEQI
                     YDVASGKVKPFDDVPEYLQTLY"
     gene            3280..4344
                     /locus_tag="VLE22_21985"
     CDS             3280..4344
                     /locus_tag="VLE22_21985"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HSB17136.1"
                     /translation="MRAKFSLFVVTAALCLGADAPLPVRGIHIAAPKPDEMPACLGFI
                     REGMPREGANLLVLEFNYRYQYTKRPEVVDSDALTREQVKEIVAACRASGVKLIPMIN
                     MLGHQSWSKTTFGLLRAHPEFDETPGKYPDNEGIYCRSYCPLHPGVHDVVFDLMDELA
                     EVSESDAFHIGMDEVFLLGENDCPRCKGKDKADLFAGEVRALHDHLAKSGRTMWMWGD
                     RFLDGEVTGIGKWEASLNSTAPSIRQIPKDIVISDWHYEWPVPTATYFAMEGFPVVYS
                     PWRKPGVALGQLELVRQARNHSPAPVAGRMLGMLQTTWCGMGPFVKAYFGEGETSRSA
                     IEAALCFRELFNEIRKGGLR"
     gene            complement(4372..4731)
                     /locus_tag="VLE22_21990"
     CDS             complement(4372..4731)
                     /locus_tag="VLE22_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17137.1"
                     /translation="MGLVQRAIEAANFSTISLSMIPELTASSGIPRLAAIEHPFGLTL
                     GLPDDAARQLAVLRGALRALVEISLPGEIVHLPFDSDFTLKVNYQPPEPPPIARYLTL
                     HPWQLLKFLNRTPPEKT"
     gene            complement(4750..5265)
                     /locus_tag="VLE22_21995"
     CDS             complement(4750..5265)
                     /locus_tag="VLE22_21995"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HSB17138.1"
                     /translation="MEHVAKVVDSYRFLDGISKRAMRRWAALGTPDQIPWTPLAKPLS
                     QCTVAIVSSAGIALRTQPPFDREIEDRDPWFSDPSFRILPRTAKAEDVRVCHLHINPS
                     FAAQDLNCVMPIERLNELEALGEIARSAPSHYSYMGYTLRPEALLHDSVPAIIRRLRE
                     EQVDTVLLVPA"
     gene            complement(5296..6528)
                     /locus_tag="VLE22_22000"
     CDS             complement(5296..6528)
                     /locus_tag="VLE22_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17139.1"
                     /translation="MKRFLFALLLAAAVPALQAEDVAGREIEPWRPGTLDIHQISTGR
                     GNAGLYILPDGTTLLVDAGELSRKTPRHTPDRPDSTRPAGEWIARYIRHALRHDPQPT
                     LDYFLLTHFHADHMGDVSDNSPASRSGAYRLTGLTLVGDSLRIGTLLDRGFPGYDYPA
                     PVEDAATRNYRAFVKWQSEHNGMKVDRFTPGRKDQIMLRRDAKKYPEFELRNVGANGE
                     VWTGLGTVTRQQFSPLDTVPRADWPSENMCSTSFRLSYGKFDFFNGGDIPGVVSPGFP
                     EWQDVETPVAKAVGPVEAAILNHHGYIDSQNAFFIGALRPRVWLLSVWDSGHPTSAVW
                     HRLQSTRLYPGPREVFATDLHPAVRLVISGIEKLSGDRGHIVVRVSPGGDDFRVVIVD
                     DSSESHKVTKVFGPYPAR"
BASE COUNT         1318 a         2132 c         2047 g         1178 t
ORIGIN      
        1 acgtcgcgca ggattgcgtt tccggagtac tcgcccttcg gagcgagcac ggcgtcaaaa
       61 tggccgccgc gggtaccctc cgaggcaacc caagccgcga gggggccggc agcaaacaac
      121 gcaatcagca tactgtcgcc ggttcgatgc acgcccgcca gccgcggccg atctcccatc
      181 ggcaatgagt gaaccgtgaa gccggagcca gggttgccgg ccagaaccgt acccgagacc
      241 catttctcca gccggctggc cagttcgatc tggttgcgga cacgggctgc ctgctcttcc
      301 gcctgttgaa gcagacgcga agcctcggga actccgccag ctctggccgc cagggcggcg
      361 atgtcctgta ggtcgttcgg gtggccgatc gccccgcgct gcaagagatc tacgagttgc
      421 gcggcaagcc cagggaagcc agcccgcaac cgtccttgcg cctccagggc cgccgcgatc
      481 tgcgccgccg cgtagagcgc gaaagacact ccatactcat ctctccgggc ggcgcagcac
      541 ccgaggagtt tctggccgct ggcctcggcc tcgcggtaat gccccgcggc cagcgagagt
      601 ttcccctggt tgagcagggc ggtggattca gaatcacgac tcgccagcaa atatgcatgc
      661 tggtagtccg gaacggcgag tttgcccgca ggagtgaaaa caaaggcgga cgcgacaatc
      721 tcggggagtg gcggcgacgg ctcgaacgga gggcgccggg cagggaggcc gctccagccg
      781 cgagcgactt cgctcgtgac ggcagcaagg tgagcaacta gtcccttgcg gattcgcgcg
      841 gtctccgcct cggctgcctg ttctccccgt ctggccccgg cttcccgctc tagctcgatc
      901 cacttgtagc cgatcacgcc gatcagtgca cagggcagaa gaatagccag taccgtgacg
      961 gtgtgcagcg agtacggacg gcccatagga ggccttctgg aaccaagtcc ataaccagtc
     1021 tatccgcaag gcgtgagaag ttgtcagaga aatgtcaaga gggttgaacc agcattcctg
     1081 atttgctctc tgcccgcgtc cccgatcgct tgtgaactca cccggctctg aatacactag
     1141 gggccatgca tcatagaaaa ggacctcata ccctcgatcg cagggagttc ctcaccgtca
     1201 ccgcgcttgc cggctcggcg ctgctcccgg cggccggctg caagaagaaa atcaagggcc
     1261 tcgtgctcac gccctccggc atgctaggcg ggctgaggca ggacggcacc gacacgccca
     1321 tcgatgacgc cacctggtac gtcgccgaaa agccgggaga cggcttccgc ttcaccttcc
     1381 ccgccggaac gcttgcgaaa gcgcagtacg tcaccaccga catgctgctc gacggcaaca
     1441 acatgatcgt cttccagctc gcactccagg aaggcgaaga cggccgcaag ttcacgttcc
     1501 gcttctccgg cctgaaccag tgctcgttcc gcgttcgcat gcccctgagc ctcgtcgatc
     1561 agaaccgatg gggcattgat cgcgaaggcg cttttctgaa gccccgcgcc ggcggttcgc
     1621 gcgtggacct cgccaaggtc gaccggatga cggtcaccgt cacccgcaaa gcaccgaagc
     1681 ccgcccgctt cgcgctgacc aatttcatcg cgaccgaaga ggagccgtcg cgcatcgacg
     1741 agcccgtgct cccgaaaggc tcgctgatcg acgagctggg ccaaagcacg ctccacgact
     1801 ggcccgccaa aagccgcggc gtcgacgagg tcgtccagcg catccgcaag cagctcgagg
     1861 aggcgcccgc ccgaaagtgg ccggacacat tctcccaatg gggcggctgg aaggcgaaga
     1921 agctgcgcaa gggcactggt tttttcacca cccactacga cggcaagcgc tggtggctgg
     1981 tcgatcccga gggttacgcg ttctggtccg ccgggctcga ctgcgttcgc gtcgatacct
     2041 cggccaacta cggcggcctc gagaccgcgc tcaaatggct gcccgacccg aagagcgaat
     2101 tcggcgacat ttacgaacac aacaccgacc tgcgccggcg gagcaatttc atcagctacc
     2161 tcgcagccaa catgatccgc gcgcttggcc gcgacggctg gcacgacaag tgggcgcagc
     2221 ttgtgtacgg ggaattacgg cgcctgcgtt tcaataccgt gggcaactgg tcggagtggg
     2281 agttcgcgcg aaaagagcag ttcccttacg tccggccgat gagcttccag ccgaaccgcg
     2341 ttcagttcgt gtatcgcgac ttccctgacg tctaccaccc cgaattcgag aaagacgccg
     2401 ccgactatgc gagcgtcctc aaggacaccg cggacgaccc cgctttcctc ggctacttcc
     2461 tgatgaatga gccgacctgg ggcttctcgt ccgaagtgcc cgccgcgggc atgctctatg
     2521 tcaccgaaac ctgcggcacg cgcaaggagc tggccgcgtc cctgaagaac aagtatccgg
     2581 acgacggagc cctcgctaaa gcctggggca tggacgtcag cttcgacaaa gtcgccgccg
     2641 gcaagtggac ggccgtgctt acgcccgaag cgcagaaaga tctcgaggcc ttcagtaccc
     2701 tcatggtgga gcgctacttc cggatcattt cgtcggcctg caagaccgtt gacccgaacc
     2761 acttgaacct cggtatgcgt tgggccggac ttcctccgtc gtgggcggtg gccggcatga
     2821 agttcttcga cgtttacagc atcaactgtt accgcgagcg cgtgcccgcc gagacgacga
     2881 agcaaatcca ggacatgctg aagaagccga ccatcatcgg cgagtggcac ttcggcgcgc
     2941 ttgacgtcgg cctgccgtca tcgggcatcg gccatctgaa gaatcaggtg gaccgcgcca
     3001 aagcgtaccg cgtttatatc gaggatgcgg ctgcaaaccc gaactgtgtc ggcgcgcact
     3061 ggttcatcct ttacgaccag tcggccctcg gccgcttcga cggcgagaac tacaacatcg
     3121 gcttcttcga tgtctgcaac cggccgtatg aagagctcgg cgccggcgcc atcgccagcc
     3181 acgagcagat ctacgatgtc gcttccggca aggtcaaacc gttcgacgac gtgccggagt
     3241 atctacaaac tctgtattga actgccggag gaatccggaa tgcgagcgaa attctctctg
     3301 tttgtagtga cggcggcgct ctgccttggc gcggatgcgc cgcttcccgt gcgcggcatt
     3361 cacatcgccg cgccgaagcc ggacgagatg ccggcctgcc tcggcttcat ccgagaaggt
     3421 atgccgaggg aaggcgcaaa tctccttgtc ctcgagttca attaccgcta ccagtacacg
     3481 aagcgccccg aagtcgtgga ctctgacgca ctcactcgtg agcaggtaaa ggagatcgtt
     3541 gccgcctgcc gcgcctctgg tgtcaagctg atccctatga tcaacatgct cggccatcag
     3601 tcctggtcga agaccacctt cggcctcctt cgcgcccatc ccgagttcga cgaaactccc
     3661 ggcaagtatc ccgacaacga aggcatttac tgccgtagtt actgcccgct ccatcccggg
     3721 gtgcacgacg ttgtttttga cctgatggac gagctcgccg aagtgtccga gtccgacgca
     3781 ttccacatcg ggatggacga agtgttcctt ctcggcgaga acgactgccc gcgctgcaaa
     3841 ggaaaagaca aagcggatct cttcgccggc gaagtgcgcg ccctgcacga ccacctggcg
     3901 aaatcgggcc gaacgatgtg gatgtggggc gatcgtttcc tcgacggaga agtgaccggc
     3961 atcggcaaat gggaagccag tctcaattcg acggcgccgt cgattcgcca gatcccgaaa
     4021 gacatcgtca tctcggattg gcactacgaa tggcccgtac cgacggcaac ctacttcgcc
     4081 atggagggat tcccggtggt atactcgccg tggcgtaagc ccggtgtcgc gcttgggcag
     4141 ctggagcttg tgcggcaggc ccgcaaccac tcccctgccc cggtcgccgg ccgcatgctc
     4201 ggcatgctcc aaacgacgtg gtgcggtatg ggcccgttcg tcaaggccta tttcggcgag
     4261 ggcgaaacca gccgctcggc aatcgaagcc gccctctgct tccgcgagct cttcaacgaa
     4321 atccgcaaag gtggattgcg gtaactcaaa gacgtgaagg ccccgcgggc ttcacgtctt
     4381 ctctggcggg gttcggttaa gaaacttgag aagctgccag gggtgaagcg tgagataccg
     4441 cgcgatggga ggcggctccg gtggctggta gttcaccttc agggtgaagt ccgagtcaaa
     4501 aggtaggtgc acgatctcgc caggcaggga gatctctacg agcgcacgca gcgcgcctct
     4561 caacaccgcg agctggcgcg ccgcatcgtc cggcaagccc aaggtcagcc cgaacgggtg
     4621 ctcgatggcc gccaggcgcg ggataccact actggcggtc agctccggaa tcatggaaag
     4681 ggagatcgtg ctgaagttcg ccgcctcaat cgctcgttgt accagtccca cggaccagca
     4741 gcagaccggt caagcgggaa caaggagcac ggtgtccacc tgctcctcac gaagccgccg
     4801 gatgatggcc ggaacgctat cgtgcagcag ggcctccggt cgcagggtgt aacccatata
     4861 ggagtagtgg gagggagctg agcgggcgat ctcgcccaaa gcttcaagtt cgttcaaccg
     4921 ttcgatcggc atcacgcaat tgagatcctg ggccgcgaag gacgggttaa tgtgcaggtg
     4981 gcacacacga acgtcctccg ccttcgcagt gcgagggagg atgcgaaacg agggatcgga
     5041 aaaccacgga tcacggtcct cgatttcccg gtcgaacggc ggctgggttc tcagggcaat
     5101 cccagccgag cttacaatcg cgacggtaca ctgcgacaag ggcttggcca gaggcgtcca
     5161 gggaatctgg tccggagtac caagagcggc ccagcgccgc atcgcccgtt tggagatccc
     5221 atccaggaat cggtacgagt cgactacttt ggccacatgt tccatacaga tttgtcttct
     5281 ctccgtgctc ccagcctatc gcgccggata cggtccgaac accttcgtca ctttgtggct
     5341 ttccgaggaa tcgtcgacaa tcaccacgcg gaagtcgtcg ccgcccggag agacccgcac
     5401 cacgatgtgg ccgcggtccc cggaaagttt ttcgattccg gagatgacga gccggaccgc
     5461 cggatggagg tcggtagcga agacttcccg gggacccgga tacagccgcg tcgactgaag
     5521 ccggtgccac accgcggacg tcgggtgccc ggaatcccag accgacagca gccacactcg
     5581 cgggcgcagc gcgccgatga agaaggcatt ctgcgagtcg atgtagccgt ggtggttcag
     5641 gatggccgcc tccactgggc cgacggcctt cgccaccggc gtctcgacgt cctgccactc
     5701 tggaaaaccg ggagagacta cgcccggaat gtcgccgccg ttgaagaagt cgaacttccc
     5761 gtaactcagc cggaaagacg tgctgcacat gttctcgctc ggccagtccg cgcgcggcac
     5821 ggtgtctagc ggagaaaact gctgccgcgt caccgtgccg agtccggtcc acacctcgcc
     5881 attggcgccc acgttgcgga gttcgaattc cggatatttc ttcgcatcgc ggcgcagcat
     5941 gatctgatcc ttgcgtcccg gcgtgaagcg gtccaccttc ataccgttat gttcggactg
     6001 ccatttcacg aaggcgcgat agttccgcgt cgcagcgtct tcgaccggcg ccggataatc
     6061 gtagccgggg aagccgcggt ccagcagcgt gccgatccgg aggctgtctc cgacgagtgt
     6121 caggcccgtg agcctgtagg cgcctgagcg ggacgccggc gagttgtccg atacgtcgcc
     6181 catgtggtcg gcgtgaaagt gtgtgagcag gaagtagtcg agcgtaggtt gtgggtcatg
     6241 ccggagcgca tggcggatgt agcgagcgat ccattcgccg gccggacgtg tcgagtccgg
     6301 acggtctgga gtgtgccgag gagtcttgcg tgacaactcc ccggcatcga cgagcaacgt
     6361 agtaccgtca ggcaggatgt acagcccagc gttgccacga ccggtactga tctggtggat
     6421 gtcaagcgtc cccggccgcc agggctcgat ttcgcgaccc gcgacatcct cagcctgtaa
     6481 cgcgggcaca gccgccgcaa gcagaagggc aaatagaaac cgtttcacgt tggcagccta
     6541 ccagttcaat tgcggcactg caaaaccgta gacccgttaa acccgtgaca gtacactaaa
     6601 ttcccacttt tcttgcctag gggcggctgc gaattgcctc gttccatcgc tcttcaagca
     6661 ataatgggac aagtg
//
LOCUS       DATIFQ010000442         6093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2065455, whole genome shotgun sequence.
ACCESSION   DATIFQ010000442 DATIFQ010000000
VERSION     DATIFQ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065455"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VLE22_22005"
     CDS             <1..896
                     /locus_tag="VLE22_22005"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HSB17140.1"
                     /translation="IQKVIKSAIVPVAGFGTRLLPATKSQPKEMLAVARKPIVQYVVE
                     ELVANCVEEILFVTGRNKGSIENHFDHDPELMRILNAGNKQELLEELKFEELQAHFFY
                     TRQRFQKGLGDAILCGENFAGEEPFVVALGDSILGLHANSSAVGRMAELFESKHASCV
                     IAVEEVPADEVRHYGVVKPTDGNGEGFRVADVIEKPKPENAPSNLAIAGRYVFSPLIF
                     DMIRRVKPDHRGEVQLTDAIRLMCEEGKRVLALKLPPGEKRYDIGNFPSYFETFVEFA
                     LADPIYGANLRKALERMLEKK"
     gene            893..2383
                     /locus_tag="VLE22_22010"
     CDS             893..2383
                     /locus_tag="VLE22_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17141.1"
                     /translation="MKSPRVVFGLLLLMLLATRLCHVGILWAEEDLPLAAAVQMVHGK
                     SLYRDFWFDKPPLTAAINLLWGAHTGWILRIAGALWVFAACLLLNEFASKLWGRREGL
                     TAACALGFFLTFGIPAAVIPLAADLLMLVPHIAAVYLAWRGRMFWSGIAAGVAMLFNS
                     KAVFVLAACALWAPRSVPALAAGFIVPHLAAIAWFGANGALQAYYEQVWKWGGIYAGN
                     TFVEQPVLNGLRRTANWGGFHAALLTGAVWFWLRDRSPERLKCALWAVLSLAAVAAGW
                     RFFPRYYFQLLPVMTLAATRGFWVLGPRRAVLLLLLLIPLARFGPRYAQLAQDLAAGH
                     PHEWRDVAMNRDSREASEIILRMARPGDTLFVWGFRPDLFAYTRLPAGTRFLECQPLT
                     GVFADRHLAQSASLEPEWTRLNRKELAATRPTFIADGLSPFNPALSLQNYEELRGWLA
                     GYKEAGRTRHTIIYRRRATSPSSTARVFRETPTSPPARPPRANSSS"
     gene            complement(2295..3071)
                     /locus_tag="VLE22_22015"
     CDS             complement(2295..3071)
                     /locus_tag="VLE22_22015"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006008.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSB17142.1"
                     /translation="MTYRNLLYEREENWAVVTLDRPQRRNALSLDLMEDLIGCLDAIG
                     KDSGIRSVILGAAGSVFSSGHDLSEMIGRSVSDYRQVFDVCAELMTKVQSIPQPVIAE
                     VQGVATAAGCQLVATCDLAVAAEEAAFATPGVRIGLFCTTPMVALSRAVGRKRAMEML
                     LTGELVDARRAAEWGLVNRVVPAGQLKAESRRLARRIAEASSFTVGLGKQAFYAQIDL
                     DQPKAYAYAKEVMTMNSLAADAQEGMSAFLEKRAPCWTGK"
     gene            3125..4606
                     /locus_tag="VLE22_22020"
     CDS             3125..4606
                     /locus_tag="VLE22_22020"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HSB17143.1"
                     /translation="MANRTEAAPARHFPPWDRAGLPDPPVFRLRHWTLLIGPGLMMVG
                     SNIGGGEWLFGPLVTAQYGGAVMWIATLSILMQVFYNLSVMRYALYCGEPIFVGFFRT
                     PPGPRFWTFFYILNDLGGLWPYLSSNAAIPLAAVVLGRLPTAADDALVRGLGYAVFLS
                     AFVPLIFGGKIYNAIEKVMVAKLVLVLGFLGFVVLFWVSADTWWEIGSGLFRFGVLPD
                     REFSWATLAAFAAVAGAGGLTNSAFSNYARDKGWGMGSLVGALPSAIGGRTIKLSHTG
                     KVFELSRENVERWRGWLKHIRRDQLVLWAPGCVLGMALPAMFSYEYIRGVQHVEGHAV
                     AAMTAEAMAARHGPALWFLTLLCGFLIMAPTQVGQLDNLARRWTDVLWTGFRPLHRLE
                     GNNVKYIYYTILAAYGAWGLIALRLTPNPLVLAVASGVMMNFALGFSALHTLYVNLSL
                     LPPALRPGWLLRIGLLTCAIFYLGISAIAFQQQWPKIASWLGV"
     gene            4607..5983
                     /locus_tag="VLE22_22025"
     CDS             4607..5983
                     /locus_tag="VLE22_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17144.1"
                     /translation="MHTESLTRRSFLQAAPLSILGAGPISFRHRGYLGWITDLASDPD
                     PNAAWPSMRLDAKLLDDYRRTLDLMKLLGFNQIIIWGLYVSRAWPVEVESAVSPGRGR
                     MVERLIESAHERGIKIFSGLGVYSWGFDDIIKARPNLHRDNPQAMCASEPESWKWMQR
                     VVDYVFKRFPIDGVSMQSADLGRCNCDRCKAFSNAEYHALLNIRVAGYIRSHWRGKTV
                     AVNSWGMQFDDPAALPALIRMSRAIDYLIDVHDTSRKRDPGYRKKLLSSLACDFGTLG
                     GPQVEPPQHWDRERWFLPVLRRSGDHLRQLSEEGGRACEYFFHILKNPGDEISFWLTG
                     RVLSDPATAWQKHLAAVVEQLYGTTAAGVRDDIAELFLRAEDAYFRHLPPSVCGTISL
                     EPLVSDRPGPPIYLRRLSAEQRRAYASELAAIRSGFQKLMSAVPHQEKVRSILRCLDN
                     TGKDLAAV"
BASE COUNT          990 a         2070 c         1853 g         1180 t
ORIGIN      
        1 atattcagaa ggtgatcaag agcgccatcg ttcccgtggc cgggttcggc acgcggctgc
       61 tgccggccac caagtcccag cccaaggaga tgctcgccgt cgcgcggaag ccgatcgtcc
      121 agtacgtcgt cgaggaactt gtcgccaact gcgtcgagga gatcctgttc gtcaccggcc
      181 gcaacaaagg ctccatcgag aaccacttcg atcacgatcc ggagctgatg cgcatcctca
      241 acgccggcaa caagcaggag ctgctcgaag agctcaaatt cgaggagctg caggcccact
      301 tcttctatac ccgccagcgc ttccagaaag gactcggcga cgcaatcctc tgcggcgaga
      361 acttcgccgg cgaggaaccg tttgtggtcg cgctcggcga ttcgatcctc ggattgcatg
      421 cgaactcgag cgcggtcggc cgtatggccg agttattcga gtccaagcac gccagttgcg
      481 tcatcgcggt cgaggaagtc cccgccgatg aggtccgcca ttacggcgtc gtgaaaccga
      541 ccgacggcaa cggcgagggg ttccgcgtag cggacgttat cgagaagccg aagccggaaa
      601 acgcccccag caacctggcc atcgcgggcc gctacgtctt ttcgccgctg attttcgaca
      661 tgatccgccg cgtcaagccg gaccaccgcg gcgaggtcca gctcaccgac gccatccggc
      721 tgatgtgcga ggagggcaag cgagtgctcg cgctgaagct cccgccgggc gagaagcgct
      781 acgacatcgg caacttcccg agctactttg agacgttcgt tgagttcgcc ctggcggacc
      841 ccatctacgg tgcgaatctg cgcaaagcgc tagagcggat gctggagaaa aagtgaaatc
      901 cccccgcgtg gtcttcgggc tgctgttgct catgctcctg gccacgcggc tctgtcacgt
      961 cggaattctt tgggccgagg aggacctgcc gctcgccgcc gcggtgcaga tggtccacgg
     1021 caaatccctc taccgggact tctggttcga caagccgccg ctcaccgccg ccatcaatct
     1081 gctgtggggc gcgcacactg gatggattct ccgcattgcc ggcgcgcttt gggtgttcgc
     1141 cgcctgcctt ctgcttaacg agttcgccag taagctgtgg ggccggcgcg agggcctgac
     1201 cgccgcctgc gcgcttggtt tcttcctcac cttcggcatc cccgccgccg tcataccact
     1261 ggccgctgac cttctgatgc tcgtgcccca catcgcggcc gtgtacctag cttggcgtgg
     1321 ccggatgttc tggagcggca tcgctgccgg cgtcgccatg ttgttcaact cgaaggcggt
     1381 cttcgtgctc gctgcctgcg cgctgtgggc gccgcgttcc gtgccggctc tcgcggcggg
     1441 cttcatcgtc ccccaccttg cggcgatcgc gtggttcgga gcgaacggcg cgctccaggc
     1501 ctactacgaa caagtctgga agtggggagg aatctacgcg ggcaacacct tcgtcgaaca
     1561 gcctgtgctc aacggcttgc gccgtacggc caactggggc ggcttccatg ccgccctgct
     1621 taccggagcc gtatggttct ggctgcgcga ccggtctccg gaacgcctga agtgtgcgct
     1681 atgggctgtg ctctcgctcg ctgccgtcgc cgcagggtgg cgcttcttcc cgcgctacta
     1741 cttccaactg ctgccggtga tgacgctcgc cgcgacacgg gggttctggg tgctgggccc
     1801 gaggcgggcc gtgctcctgc tcctcctgct gattccgctc gcccgcttcg gcccgcgcta
     1861 tgcgcaactg gcgcaggacc tagccgccgg ccatccacac gagtggcgcg acgttgccat
     1921 gaatcgggac agccgcgagg cgtcggagat catcctgcgg atggctcgtc ccggcgacac
     1981 cctcttcgtc tggggatttc gccctgatct cttcgcctac actcgtcttc ctgccggaac
     2041 ccggttcctg gagtgtcagc cgctcactgg cgtgttcgcg gaccgccacc tagcgcagtc
     2101 cgcttctctc gaacccgaat ggacacgcct caaccgcaag gagctcgccg cgacgcgccc
     2161 aacgttcatc gcggacggcc tgagcccatt caacccggcg ctatcgttgc agaattacga
     2221 ggagctgcgg ggctggcttg ccggctacaa agaagccggg cgcacccggc acacgatcat
     2281 ctaccgccgg cgcgctactt ccccgtccag cacggcgcgc gtttttcgag aaacgccgac
     2341 atcccctcct gcgcgtccgc cgcgagcgaa ttcatcgtca tgacctcttt ggcgtaagcg
     2401 tacgccttcg gctggtccag atcgatttgc gcgtagaacg cctgcttgcc aagtcccacg
     2461 gtgaaactgc tcgcctccgc gatccggcgc gccaaccgcc gcgactcggc cttcagttga
     2521 cccgcgggaa cgacccggtt cactagcccc cactccgccg cccgccgcgc atcgaccagc
     2581 tcgccggtga gcagcatctc catcgcgcgc ttgcgtccga cggcgcggct cagcgccacc
     2641 attggtgtcg tgcagaacag gccgatccgc acccccggcg tcgcgaaggc cgcttcttcg
     2701 gctgccactg ccaggtcgca ggtcgccaca agttggcacc ccgccgccgt ggctactccc
     2761 tgcacttcgg cgatcaccgg ctgcgggatc gactggacct tcgtcatcag ctccgcgcag
     2821 acgtcgaaca cctgccggta gtcgctcacg ctccgcccga tcatctccga caggtcatgt
     2881 ccggaggaga acaccgagcc ggctgctccg aggatcaccg agcggatgcc ggagtctttc
     2941 ccgatcgcgt cgaggcaccc gatcagatcc tccatcaggt ccagcgaaag cgcattgcgc
     3001 cgctgcgggc ggtccagagt cacaaccgcc cagttctctt cccgttcgta cagaaggtta
     3061 cggtatgtca tacaacccag gcccagtttc cactatagtg ccgccactga tacgatggcc
     3121 gctcatggcc aaccgtaccg aggcggcgcc ggcgaggcac tttcccccgt gggaccgggc
     3181 cgggctcccg gacccgccgg ttttccgcct ccgccactgg acgctgctca ttggtcccgg
     3241 gctgatgatg gtcggctcca acatcggcgg cggcgagtgg ctgttcggcc cgctggtgac
     3301 ggcgcagtac ggcggcgccg tgatgtggat cgccacgctg tccatcctga tgcaggtgtt
     3361 ctacaacctc tcggtgatgc gatacgcgct gtactgcggc gagcccattt tcgtcggatt
     3421 cttccggact ccgcccggac cgcgattctg gacgttcttc tacatcctca acgacctcgg
     3481 cggcctctgg ccctatctct cgtcgaatgc cgcgattccg ctcgcggctg tggtcctcgg
     3541 ccggctcccg accgccgccg atgacgcgct tgtaaggggg ctcggctatg cggtgttcct
     3601 ctcggcgttt gtgccgctga tcttcggcgg taaaatctac aacgcgattg agaaggtgat
     3661 ggtggcgaag ctggttctgg tgctcggctt cctcggcttc gtcgtgctct tctgggtgag
     3721 cgccgatacg tggtgggaga tcggctccgg gctgttccgc ttcggcgttc tgcctgaccg
     3781 cgagtttagt tgggccaccc tcgctgcttt cgccgccgtc gccggagcgg gaggtctcac
     3841 caacagcgcg ttctccaact acgctcgcga caagggctgg ggcatgggat ccctcgtcgg
     3901 agcgcttccc agtgccatcg gcggccgcac catcaaactt tcccataccg gcaaagtctt
     3961 tgagctttcc cgcgagaacg tggagcgatg gcgcggatgg ttgaagcaca tccgccgaga
     4021 ccagcttgtg ctgtgggcgc ccggctgcgt gctcggaatg gccctgccgg ccatgttctc
     4081 ctacgagtac atccgcggcg tgcaacacgt ggaaggacat gccgtcgcgg cgatgacggc
     4141 cgaggccatg gccgcccgcc acggaccggc gttgtggttc ctgaccttgc tctgcggttt
     4201 tctgatcatg gccccaacgc aggttggcca gctcgacaac ctcgcccgcc gctggaccga
     4261 cgtgctatgg acgggcttcc gccctctcca ccggctcgag ggtaacaatg tgaagtacat
     4321 ctactacacg atcctcgccg cctatggagc ctgggggctc atcgccctgc ggctgacgcc
     4381 gaacccgctc gtgctggccg tcgccagcgg tgtgatgatg aacttcgcgc tcggcttctc
     4441 ggcgcttcac acgctctacg tcaacctgtc gctgctcccg ccggccctgc ggcccggctg
     4501 gctcttgcgc atcggccttc tcacctgcgc gatcttctac ctcggcatct ccgccattgc
     4561 gttccaacag cagtggccga aaatcgcttc gtggctgggg gtgtaaatgc atacagaaag
     4621 cctgacgcgc cgttcgtttc tacaagcggc tccgctttcc atccttggcg cgggcccaat
     4681 ctcattccgc catcgcggct accttggctg gatcacggat ctggcctcgg atcccgaccc
     4741 gaacgctgcc tggccctcca tgcggctcga cgcaaagttg ctcgacgact accggcggac
     4801 gctggatcta atgaagctcc tcggcttcaa ccagatcatc atctggggac tgtatgtctc
     4861 gcgggcgtgg cccgtcgagg tagaaagcgc ggtctcgccg gggcgcggaa ggatggtgga
     4921 gcggctcatc gaaagcgcgc acgagcgagg tattaagatc ttctcgggtc tcggcgtcta
     4981 tagctggggc ttcgacgaca tcatcaaggc tcgtccgaac ttgcaccgtg acaatccaca
     5041 ggccatgtgt gcgagcgagc cggaatcctg gaagtggatg cagcgggtgg tggattacgt
     5101 ctttaagcgc ttcccgatcg acggtgtgag catgcagtcg gcagacctgg gacggtgcaa
     5161 ttgcgatcgc tgcaaagcat tcagcaacgc cgagtatcac gccctgctca acatccgcgt
     5221 cgccgggtac atccgcagcc actggcgcgg caaaaccgtg gccgtcaaca gttggggcat
     5281 gcagttcgac gacccggcgg ccctcccggc actcattaga atgagccgcg cgatcgacta
     5341 tcttatagac gttcacgaca ccagccggaa acgggacccg ggctacagaa agaagctgct
     5401 atcgtcgctc gcgtgcgact tcggcacgct cggcggcccg caagtggaac cgccgcagca
     5461 ctgggaccgg gagcgctggt tcctccccgt actgagacgc tccggagacc atctacggca
     5521 gctctccgag gagggcggcc gcgcctgcga gtacttcttt cacatcctga agaacccagg
     5581 agacgaaatc tccttctggc tcacaggccg ggttctttcc gatccggcaa ctgcctggca
     5641 gaaacatctg gcagcagtcg tggagcaact ctacggcacg accgcggctg gcgtcaggga
     5701 cgatatcgcg gaactgttcc tccgcgccga agacgcctac ttccgtcatc tgccgccgtc
     5761 ggtctgcgga acgatctcgc tggaaccgtt ggtaagcgac cggccgggtc cgccgatcta
     5821 ccttcgccgt ctgtctgctg agcagcggcg agcttacgca tcggagctgg cggcgatccg
     5881 ctcgggtttc cagaagctca tgtccgccgt tccacaccag gaaaaagtgc ggtcgattct
     5941 cagatgcctg gacaacaccg gcaaggatct cgcggcggtt tgagtctcgg gttcgattct
     6001 atttcttccc cgcgatctgc cactccgtga agggcgagcg cgcaccgcgg atgttcacgt
     6061 tcagaagata cggccgtccc gacgcgaatg ccc
//
LOCUS       DATIFQ010000443        16665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_146829, whole genome shotgun sequence.
ACCESSION   DATIFQ010000443 DATIFQ010000000
VERSION     DATIFQ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16665
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146829"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VLE22_22030"
     CDS             complement(<1..1065)
                     /locus_tag="VLE22_22030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB17145.1"
                     /translation="MKVVLLGPYPPPHGGVQTNLVAIRDYLRARNVHCDVINLTRFRR
                     PDGDGVHYPANPVQVARLLVRGRYDVIHLHIGGELKRRLLALCFFCSLLPRVKTVLTF
                     HSGGYPSSRPGRTVHRTSLRGFVLRRLDCVIGVNPELVDFFGRFDIAGERIRLIPPFS
                     LPSGPPDAEYPRVLDEFVRDHHPLIVTVGQLEPEYDLFLQIAALDALRSRLPAAGLVI
                     AGSGSLERELRSRIASQPDQGRVLVCGDLPHPVTLRLIAECDVFLRTSLYDGDSIAVR
                     EAIHFGAPVIATDNGLRTGGVHLIALHDLDGLCRAIEACQAGPERSKRHPGIEHWGNV
                     EAVWELYNELTDARRDSEDPG"
     gene            complement(1062..2216)
                     /locus_tag="VLE22_22035"
     CDS             complement(1062..2216)
                     /locus_tag="VLE22_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17146.1"
                     /translation="MGTCRRLLLLLLVLYLGGSAYRICVRRYHIWLPDYVRWSLQREA
                     QPMRPVHVFFLVADHFEPATRQDFLARWEREYPRLADRHRDSNGRAAQHTWFYPGEQP
                     INSNLEALRRLVAGGYGEVELHYHHGGDSIESARRKFSAAIEWFQHYGFLRTADGRTN
                     FAFVHGNWALDNSEDPSRCGVNRELALLRELGCFADFTFPALWREAQPAWVNRIAMAT
                     DDEKPKSYDRSAPVRVGSAPSGDLLIFQGPLQIYPVWDPRWLWWRVEDGDIHPSVPVN
                     ERRVDLWIRANIHVSGKPDWIFVKVHGHGASSDGDAGEFLGRHFDRALSHLESRYNDG
                     VNYILHYVTAREAYNLICAAEAGVTGQPERYYDYVIKPYVANAGSHTASK"
     gene            2353..3849
                     /locus_tag="VLE22_22040"
     CDS             2353..3849
                     /locus_tag="VLE22_22040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB17147.1"
                     /translation="MSTACSGPQQEKSNGPISGPQNTRIAFCVLIVIGIFHFATIRDG
                     HSWGDDFAQYILHARNIVEGRPYGDTGYLGNPYNIDLGLPNYPPLTPLALAPLYALFG
                     ASLVAFKTEMVVLLLGALTAIYAFVRTREGNFRALAVAALIGLNPYFFDAKNEIGSDF
                     LFLLLTYLCFLLIEKAGAHDEKPRGLGALIGILGYLAYATRSLGALLLPAAIMLDFKR
                     FRSLRKTTLLAVSIAVILICFHGILLRSEVDYSLVLIELFSFRTAVLNVRQYVAELGI
                     LWSNGYSPIVQKFFYLLTLPVAATGLMRTFRRRAGIGECFLPLYLATIVLWPANQGIR
                     FLIPLMPLYMNYLLDGAEWLIARTGPVRRFVHAGLCLTILFTYSSAYARKEYGPIRGG
                     IQEPEFLRMVSYVRQHTRTEDRFLFRKPRAFTLMTGREAAVYNRREHKARMWEFVRAA
                     GIHYVVVPKYDLDFNSGQMDMAAFIDENPEYFVETYANTRYSVYRIIL"
     gene            complement(3934..6396)
                     /locus_tag="VLE22_22045"
     CDS             complement(3934..6396)
                     /locus_tag="VLE22_22045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17148.1"
                     /translation="MRRIAAFAVCLLLASGCAHTQTSLSVSPSEVVFRSVAGGFQDVP
                     QKVNLTASGNWTASIVGEVPWLNVKPEKGQASGTLTLSLVDWAAEALASGDHTAEVVV
                     AQGDSSQRIKVTATIVPQAPKPNFSYLGGPKFCLQPPGYDDQATCTVPDEKPPGAFVP
                     PRLGGSYVDPNFGALVRVIAPPRSVHSYSTPSAVSAQNKYVLVNMDGGWSVVSPVSGK
                     VLHRNIPVVEGSMWDARDPEILYFISGTTVNKYTLTTGKTSTLVDYAKTTPRFASITA
                     GGAGDTSKDNWISFYAPQEKIVCALDLNTVKTYCGSFQNVGRVTLDPSGRGTLIAKGV
                     DRPTGKRYIMLVAYPTMAFFSVNLAAERLDFEFLAPERPDQNGNLDGVCDAGERCYVG
                     EHNDTFEDAGGVQYLIGDVDPMTPCSYAVVRLQINKGKDMLRPVELGGGLRKFITLFR
                     CSSGDVWTDLHYGCAKQSAYCVVSTTYGRTGYQKDINDLNPIRRTAHLSEIMVIKGDG
                     SEIRRLAEHRSVPLSTEEALSYWSTPRASISEDGAYVVADSNFGEVEKQRVILIETGY
                     GKTRIAALADAATFAPTISPGGIASIFGLNLANCNDAPGLIPLPEEYCGTTVRFGILP
                     ARFFYASTGQLNVQVPRSLPPSAETFVTVSRGSELSDSDTVLLPASAVSPVSPGIYSY
                     SLEDDVPRAVVQNVDPITKEYSLNGPLRPEYGIRPQLPGEAGVVYANNLGATSPEVAD
                     GHPPESPLTLTRSTIDVYINNVRQSTFFAGLAPLFVGLYQVNYWLAPETPVLDNDQNR
                     IWLRAGGLESPPLNVSLASDSK"
     gene            complement(6474..7514)
                     /locus_tag="VLE22_22050"
     CDS             complement(6474..7514)
                     /locus_tag="VLE22_22050"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB17149.1"
                     /translation="MEEAPGRVRPCVECYGVEIVQGILRCDACRVEFPIVDGVPRFNP
                     DVEQDNPDFFRQYAQAFRYRRPADGDFQALHSETKRSFGFQWLRFRVTDHAENRALFC
                     RRTGTTPGSLGTQLFFEAGCGMGRYLQVIADEAPSEVVGLDLSLATNRAYEENRRNPF
                     VHVVQGDILRLPLRPASFDHVYSIGVLHHTPDTERAFRSIVKLAKPGGSVSIWVYHVW
                     RPPNLHGLKAVHASLKGWIADTLRGITTRLPLSVLCYLCYVAAPVGWLLRQIDRLPAP
                     ARLLFSPLLLIPCSVHPRWEVRVLDTFDWYSPRYQWKHTVAEAESWFRDAGLEEISTE
                     GFPVTVRGRRPV"
     gene            7749..9326
                     /locus_tag="VLE22_22055"
     CDS             7749..9326
                     /locus_tag="VLE22_22055"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HSB17150.1"
                     /translation="MAQATTADGQSRRSDRLVSGVLWNWAGIGVQLFTGVIVSPYIIR
                     KLGTERYGIWALVFSLASYYGFIDLGFRSAVVRFSAHYYTRRENPKVNELINTTFFYF
                     SLAAAMLVCLTLILWKLADRIFRVSPALRDEFSWLVLIVGISISVGLNMSVFSGVVEG
                     LQRFDISNRIRIVVFGLRGAGWFLLLMAGYGLVAMGWWSLATSVVFFFLYLLYLRRIF
                     PALEFSRQFVTLSMFRETVSYGLHTFLASIGTQSLDQTPSLLIGYFRRAADVGYYNFP
                     LRVIQYVSQAITNVGVVALPHAASLAAEGRLDKVANLGIYANRYCLSLFMPLAVFLTV
                     YGTELFSIWVTPEFARNAVYLLPPLLAGFGLGQAAQFCTGSILFGLGEQKMYARAIVG
                     EAAASIVFMVAVIPRYGVYGAAVVTASLMTVTRGCIGPWILCLRLGFSFQRYMQSILA
                     PPLAASVPAWLALWGLKRWGVLGKNWSELILAGGVVCALYGVTALYLCVSKDHRRLLF
                     NWAGQRLGRRDGPQRHS"
     gene            complement(9271..11061)
                     /locus_tag="VLE22_22060"
     CDS             complement(9271..11061)
                     /locus_tag="VLE22_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17151.1"
                     /translation="MRASWTYPAASRQVRIYREAWRFLLIEGQPDRYPRENEPLDQWL
                     DGRWGSFRGFEILAGLPARLTAFVDPLATRPVFFVPTGGGSLVSDKLSTLALNRTGEL
                     SPNWPAILEALTLGTLYTTDTSIAQAQQFQPGEVIQFRQDGIVVRGRFEMPLDPELSS
                     HRIAEDPPGTLLAALRRAVAETWVERDSYLLLSGGLDSRLTLSQAGSDRKAMTVTTRE
                     NRECRIARDIAGACGAKFLHWPRPPEHYVTLLESSFLLTACMWDPLTPHHLGLGVEWR
                     KEGIHEVCNAFLYDTVLKGYFAFPRQKYVSPYLPLHDVLGNSAVIFAEKRSGRSHRAT
                     SEFVLQSLTKEARQIAAQRLKDLANSMEPAVAGDLDLTLQNRLIAFISRQCHYPLLLG
                     WIEELDVNSPIFHRALWSWARLSPPDAYYEARAFRAALAGAGHPVAGLPDSNTGRSSG
                     RVSRDWRATFRNQAWYPLARTAWRKLRPVSDGSAASGAVYLDEGSWPPLSPLFRTPLG
                     SEALEQGLCLLDGVPLFRKHEVETILQWFRSGDDAPLEALLSLSALGRWLNYLRSASA
                     GASSGRLPAARRITNAAADHLAGPDAVLPS"
     gene            11268..12146
                     /locus_tag="VLE22_22065"
     CDS             11268..12146
                     /locus_tag="VLE22_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17152.1"
                     /translation="MATGQLELELSDVRGANLDDTVRIELFDEQGNCDYRNREQVARQ
                     IILTGIESSPLTFYRVAVTPTRYRIRQAFVAVKEGKTVTLNLSFPVEPRKVKDIQARP
                     FPDLAVSLQAVLEISDIPAYRDDKGNPLQGEELYEALKPRDRACLLNLYAKASNTVLG
                     DGKSCFDHFGGLTLLHQDRFFARTHGALEEEAAVSPLFREESGALHEPPAGYTLGRSF
                     KTRDEHGNLQLTFFRRGDTGDDYLVDVDIDEANGIEHIFEVVRNQVTGRTNPYDVREI
                     LIASQGLDPGYSFVFG"
     gene            complement(12301..12579)
                     /locus_tag="VLE22_22070"
     CDS             complement(12301..12579)
                     /locus_tag="VLE22_22070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17153.1"
                     /translation="MDVGIRPLFSVMLALVGFAVPPPLRAQQAPKAISAEELKTLIDK
                     KVKFFFLDVREPAEIKALGSMKGYVNIPLSQLGVRLPEIPKNVLIVTA"
     gene            complement(12613..14049)
                     /locus_tag="VLE22_22075"
     CDS             complement(12613..14049)
                     /locus_tag="VLE22_22075"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HSB17154.1"
                     /translation="MRELQPRRVLTVAPMPPEKCAYALARYSRSPDSIRQSLEWVRTH
                     DSQKFLEHFYFQYGHASIADLGHVVLCFEGISEIAATEIEDEPLWDGQAKSSRYQDFS
                     TAGFITPQEFGEADAAVYRTAAETLLGAYSRIHAAVRDYLAGQLPRPESMKPDAYERN
                     IAARAFDVARHLLFLGVPTNVGQVTSIRTLEKQIRRLGASEYEELRSLAAEIAESCAS
                     VPACALDDSVVHEPVAPTLARFVDADRYLAQSRADLKLWVDQNLPPAAGVTPEPVDLL
                     KPSDTSADIVATLLYPVTDRPFRELYELSCSWSAERRREVIDLAVRSRTRRDELLREF
                     QGGLYAFDMIIDIGAYRDLHRHRRCHQYRQTYNGNLGFETPKAIVDAGLRETYEAALR
                     KALEVRRSLPQPGSHYLIPFGACSRFLFKMDFAEAEYISRLRSGVKGHFSYREVAWRM
                     KQKMDELEPELGRLINATPPWVEDPLKR"
     gene            14079..14510
                     /gene="dut"
                     /locus_tag="VLE22_22080"
     CDS             14079..14510
                     /gene="dut"
                     /locus_tag="VLE22_22080"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HSB17155.1"
                     /translation="MKVRIQKLRPDAFLPEYAHGPDEDAGMDLRAVEDVVLEPGVPRA
                     VPTGLAIELQPGYEAQVRPRSGLALKHGITLPNAPATIDPGYRGEIKVILLNLGRESY
                     RVHVGDRIAQMVIARYERIEWEEGMLNNSRRGTGGFGSSGR"
     gene            14622..15626
                     /locus_tag="VLE22_22085"
     CDS             14622..15626
                     /locus_tag="VLE22_22085"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSB17156.1"
                     /translation="MYKIFQFPANQIPRVDGRTDDWDMVPADYVIGTDQLTDSKGNLK
                     HDPRDIDVKVRVGWVKGLNRLYFLYEAYDNFWAFDRPDEHNDLFEVVVDGDLSGGPLI
                     ADLHPHKELDPWGIYRAVQGVHAQNWHVFTPAEGKDWVMVWGCQQPWIRELPYGNAAY
                     SYNFKHGESGHLILEFWLTPFDYANCDGGPAASVESVLRENKLIGMAWAILDYDSDSG
                     PYKAFWNLSPKITMYGNASDLVAFRLMPLEPRFRKPIEAQWSFKILDMERRLVAFQDL
                     SEGDITSWTWDFGDGTTSNARHPIHQYQQPGQYITVTLRVEGPAGKDRRAKVFDVVLK
                     "
     gene            complement(15655..16131)
                     /locus_tag="VLE22_22090"
     CDS             complement(15655..16131)
                     /locus_tag="VLE22_22090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17157.1"
                     /translation="MRQASSIFAIVFLLALLSFGQFTNPVQGSSPDTPADLTNAMPVL
                     EVFHSTTAAMEGAPSSRVDASREGTERRLLTPEEARKSRLLITRVADKFYWASRENRE
                     LEALHSGASTYFYPRDSPGNYIKMTRVGAKIYYLEHVSLWLSTITYWGELQIIPAK"
     gene            complement(16195..>16665)
                     /locus_tag="VLE22_22095"
     CDS             complement(16195..>16665)
                     /locus_tag="VLE22_22095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17158.1"
                     /translation="PTAEMYRWVSFETSTNRFTIYPAALGSTLVLLDDENFRNNIWYR
                     EGTPSWFYNTPYPLKTTQRDWIEWTPNVVTSRVRVEGEKARVSLASFTPNFKTFQMKS
                     GEGTWRNCEDELDVPLVKGTNRFVFRTVNLFEVTGPEHRVQIDWLDNAERPSGH"
BASE COUNT         3489 a         4851 c         4980 g         3345 t
ORIGIN      
        1 cccagggtcc tcagagtctc gccgcgcgtc cgttaactcg ttataaagct cccacacggc
       61 ttcaacgttg ccccagtgct cgatacctgg gtggcgtttc gagcgctccg gtccggcttg
      121 gcacgcttca atggcacggc aaagcccatc cagatcgtgc aatgcaataa gatgtacacc
      181 accggtgcgc aagccgttgt ctgtcgcgat aaccggagcg ccgaagtgga tggcctcgcg
      241 cacggcaata gagtcgccgt cgtagagtga cgtgcgaagg aatacgtcgc attcggcgat
      301 cagacgcaag gtgactggat ggggcagatc gccgcagacg agaacgcggc cctgatcagg
      361 ctgagacgcg atccgcgaac gcagttcccg ctcgagactg ccggaaccgg cgatgaccag
      421 tccggcggcg ggaagccgcg atcgcaacgc atcgagcgca gcgatctgga gaaaaaggtc
      481 gtactcgggt tccaattgtc cgaccgtcac gatgagaggg tgatgatccc ggacgaactc
      541 gtcgagtacg cggggatatt cggcgtcggg cggcccggac ggaagagaga acgggggtat
      601 cagacgaatc cgctcgccgg cgatatcgaa tcgcccaaaa aaatccacga gttccgggtt
      661 gacgccgatg acacagtcaa gccggcgcaa cacaaagcct cggaggctcg ttcggtgcac
      721 ggtacgaccg gggcgggagg acggataccc gcccgagtgg aacgtgagta cggtcttcac
      781 ccggggaagc agcgagcaga agaagcacaa cgccagaaga cgacgtttca gctctccgcc
      841 gatgtgcagg tgaattacat cgtagcggcc gcgaaccagg agacgggcga cctgcacagg
      901 atttgccggg tagtggactc cgtcgccatc cggccgtcga aacctggtga gattgatgac
      961 gtcgcaatgc acattccgag cgcgaaggta gtcacggata gcaaccaggt tggtctgcac
     1021 gccgccgtgc ggaggtggat aaggaccgag tagaacgact ttcatttcga tgcggtatgg
     1081 cttccggcgt ttgcgacata cggtttaatc acgtagtcgt aataccgctc cggctgaccg
     1141 gtgactcccg cctccgcagc acaaatgaga ttgtatgcct cacgagccgt aacgtagtgc
     1201 aggatgtagt taacgccatc gttgtagcga ctctccaaat gagacaatgc ccggtcgaag
     1261 tgccgtccta agaactcccc ggcatcgccg tctgacgaag ctccgtgccc atgaaccttg
     1321 acgaaaatcc agtccggctt acccgatacg tgaatattgg cacgaatcca caaatcgacg
     1381 cgacgttcat tgacgggtac tgaaggatgg atgtcgccat cctctactct ccaccacaac
     1441 cagcgcgggt cccagacggg gtagatctgt aggggaccct gaaagatcaa cagatcaccg
     1501 ctgggagcag agcccacacg cactggagcg ctcctgtcat aggacttcgg tttctcgtcg
     1561 tctgtggcca tggcgatcct gttcacccag gcaggctgcg cctcacgcca gagcgccgga
     1621 aatgtgaagt cggcaaagca gcccaactca cgcaagaggg ccagttcacg gttcacaccg
     1681 catcgcgatg gatcttcgct gttgtcgagc gcccagttgc cgtgaacgaa ggcgaagttc
     1741 gtcctgccgt cagccgttcg caagaaaccg taatgctgga accattcgat ggcagccgag
     1801 aacttccggc gggctgactc aatgctatcg ccgccgtgat ggtagtgaag ttccacctcg
     1861 ccgtagccgc cggccacgag ccgacgaagc gcttcgaggt tcgaattgat cggctgctcc
     1921 cctggataga accaagtgtg ttgggcggct ctgccgttac tgtcccgatg gcggtcggcg
     1981 aggcggggat attcccgttc ccatcgagcc aagaaatcct gacgagtcgc aggctcgaag
     2041 tgatcggcga caaggaaaaa aacgtgaacc ggccgcatcg gttgtgcctc acgctgtaag
     2101 ctccaccgaa cgtagtcggg cagccagatg tggtagcggc ggacgcagat cctgtatgcg
     2161 ctgccaccca ggtatagcac caaaagcagc aatagcagtc tgcggcacgt gcccatgtgt
     2221 tgtctcaagg aagatgtcca ggcaccggtc tcaccccgat ccccgctatt ttatcgtacg
     2281 gcttgcctga cccgcaggca tgccgagaaa cccgaacgtg cactgtatac tgaacgtcag
     2341 gccataaccg gaatgtccac cgcgtgctca ggaccccaac aagaaaagag caatggaccg
     2401 atatcgggac cgcagaacac ccgcatcgca ttttgcgttc tgatcgttat tgggattttt
     2461 cacttcgcca caatcaggga cggacacagt tggggcgacg actttgcaca gtatatcctg
     2521 cacgcccgaa acattgtaga gggtcggccg tacggcgaca caggctacct cggcaatcct
     2581 tataacatcg atctaggtct accgaactat ccgccactca ccccgctcgc gctcgctcct
     2641 ctctatgccc tatttggcgc atcactggtt gccttcaaaa cggagatggt tgtgcttcta
     2701 ctcggcgcac tgacggcgat ctacgccttc gtccggacac gtgaaggaaa cttccgggcg
     2761 ctggccgtgg cggccctgat cggactgaac ccctactttt ttgatgcgaa aaatgaaatc
     2821 ggctcggact tcctttttct tttattgacc tatctctgct tcctcttgat cgagaaggca
     2881 ggcgcgcacg atgagaagcc acgcggactt ggcgcactca tcgggattct aggctacctt
     2941 gcttacgcca cacgaagcct cggcgcactc ttgctgcctg cagccattat gctcgatttc
     3001 aagcgattcc gaagcctcag aaagactacg ttactcgcgg ttagcatcgc tgtaatccta
     3061 atctgttttc atggcatatt acttcgatct gaagttgatt actcgcttgt gcttatcgag
     3121 ttattttcgt tccgtacggc agttttaaac gttcgacaat atgtagcgga gcttggcata
     3181 ctgtggtcaa atgggtacag cccgattgta cagaagttct tctatcttct gactcttcca
     3241 gtggccgcca ccgggctgat gcggacgttt cggcggagag cgggcattgg tgagtgtttc
     3301 cttccgctgt atctagccac cattgtcttg tggcccgcga accaaggcat ccggttcctg
     3361 ataccactca tgccactgta catgaactat ctactggacg gtgccgagtg gctcattgca
     3421 cgcaccgggc cagttcgccg gtttgtacac gcgggattgt gtctcacgat tctctttacg
     3481 tattcctccg cctatgcccg aaaggaatac ggcccgattc gaggcggcat tcaagaaccg
     3541 gagtttctgc gcatggtttc gtacgtgcga cagcatacga ggacggagga tcgtttcctc
     3601 tttcgcaagc cacgggcatt caccctgatg acaggacgcg aagcggctgt ctacaaccgt
     3661 cgtgagcaca aggccaggat gtgggagttt gtgcgagcag ccggcataca ttacgtcgtg
     3721 gttcctaaat acgatctaga cttcaacagc ggtcagatgg atatggcggc gtttatcgac
     3781 gaaaacccgg aatattttgt tgagacgtac gctaataccc gttattcggt ttaccgaatc
     3841 attctttgaa ctgggcgcgt cctgacagac atcggccttc gaatgcatca gaagcgtcga
     3901 gaaatgtggg gggcacactg atggggtcaa gattcacttc gaatcactgg cgagactgac
     3961 gttgagagga ggactttcaa gaccaccggc ccgcagccag atcctgtttt gatcgttgtc
     4021 aaggaccggt gtttctggag ccagccagta attgacctga tacaggccga cgaataacgg
     4081 agcaagaccg gcgaagaaag ttgactggcg aacattatta atgtaaacgt caatggtcga
     4141 tcttgtcaga gtcagcggac tctcgggagg atgaccgtcg gcgacttccg gggacgtcgc
     4201 tccaaggttg ttggcgtaga ccacgccggc ttcgcctggt aactgcggac gaataccata
     4261 ctccggacgt agcgggccgt tgagggagta ctctttggtt atcgggtcga cgttctggac
     4321 gacggcgcgc ggaacgtcgt cttcaaggga gtacgagtaa ataccgggcg acaccgggga
     4381 aacggcggaa gcaggcagca gcaccgtgtc ggagtcggat agctcgcttc cccgggacac
     4441 agtgacgaac gtttccgcgg agggggggag ggaccgcggt acctgcacat tcaactgacc
     4501 agtactggca tagaagaaac gagccggtag gatcccaaac cggactgtcg tgccgcaata
     4561 ctcctcaggg agtggaatca ggcctggagc gtcattgcag ttcgccaggt tcaagccgaa
     4621 gatcgatgca attcccccgg gggagatggt tggagcgaac gttgccgcgt cagccagggc
     4681 ggctatccgg gtctttccat acccggtctc gatcaggatt acgcgctgct tttcgacctc
     4741 tccaaagttt gaatcggcca caacatacgc tccgtcctcc gagatgcttg ccctcggcgt
     4801 cgaccaatat gacagagctt cttccgtgct caagggcaca ctccgatgct cggcgagccg
     4861 gcggatctca gatccgtcgc ccttgatcac catgatctcc gacaggtgag ccgtacgcct
     4921 aatggggtta aggtcattga tgtctttctg atagccggtc ctgccgtacg tggtggagac
     4981 gacgcagtat gccgattgct tggcacagcc gtagtgcaga tccgtccaca catccccgct
     5041 cgaacaacgg aagagcgtga tgaacttcct caacccaccg ccgagttcca ctggacgaag
     5101 catgtcctta cccttattga tctgaaggcg gaccaccgcg taggaacagg gcgtcatcgg
     5161 atccacatct ccgatcaggt actgcacgcc tccggcgtcc tcgaacgtat cgttatgctc
     5221 gccgacgtaa cagcgctcac cagcgtcgca aacaccgtcc aggttcccat tctggtcagg
     5281 tctctctgga gcgagaaatt cgaagtcgag ccgttcggcg gcgaggttga cggagaaaaa
     5341 cgccatcgtc ggataggcga caagcatgat ataccgctta cccgtcggcc gatccacccc
     5401 ttttgcaatc agggtacctc tcccactggg gtcgagagtt actcggccca cattctggaa
     5461 gctcccgcaa taggtcttta cggtgttcag atccagcgcg cagactatct tctcctgcgg
     5521 ggcatagaac gaaatccagt tatcctttga ggtatcccca gcgccgccgg cggtaatcga
     5581 tgcgaacctc ggggtggtct ttgcgtagtc taccaaagtg ctggttttcc cggttgtgag
     5641 ggtgtactta ttgacggtcg taccacttat gaagtaaaga atctcgggat cgcgtgcgtc
     5701 ccacatcgag ccctctacga ccggaatgtt tcggtgcagc accttcccgc taaccgggga
     5761 aacaacgctc caaccaccgt ccatgtttac cagaacgtat ttgttctggg cactcacggc
     5821 gctcggagta gagtaggagt gaacggacct aggcggagcg atgacacgga ccaacgcgcc
     5881 gaaattcgga tccacgtagg acccgccgag cctgggcggc acgaatgcgc ccgggggttt
     5941 ctcgtcgggc accgtgcagg tcgcctggtc atcgtagccg ggaggctgaa gacagaattt
     6001 cggaccgcca aggtagctga agtttggctt gggagcttga ggaactatag tcgcggtgac
     6061 cttgattctc tgcgaggaat cgccctgggc gacgacgacc tcggcggtgt gatctccgga
     6121 tgcaagtgcc tcggccgccc agtcgacgag ggaaagcgtc agagtacccg aggcctgacc
     6181 cttttccggc tttacattca accacggaac ctcgcccact atgctcgcgg tccagttccc
     6241 agatgccgta aggttgactt tctgcgggac atcctgaaac ccgccggcca ccgatcggaa
     6301 gacgacctcc gagggcgaaa cagacaagct tgtttgcgta tgggcgcagc ctgaggcgag
     6361 cagcaggcag acggcgaaag ctgcgatcct ccgcatgtag agattatatc cgagcaacgc
     6421 ttgccggcct gttcaggact cacaaccaga tgcctcgcgt tcaaaggggc tcgtcaaacg
     6481 ggccgcctcc cccggaccgt gacggggaaa ccctctgttg agatctcttc caaaccggca
     6541 tcgcgaaacc aactctccgc ctcagccacc gtatgcttcc actgataccg cggagagtac
     6601 cagtcgaaag tatccagaac ccgaacctcc cagcggggat gaaccgagca agggatcaac
     6661 agcagaggcg agaacagcag ccgtgccggc gccggcagtc tgtcgatctg cctgaggagc
     6721 cagccgacag gcgccgcgac gtagcagaga tagcagagta ccgagagtgg aaggcgagtc
     6781 gtgattccgc gcaaggtgtc ggcaatccag cccttcagcg aggcgtgaac cgcctttaga
     6841 ccgtgcaaat tcggcgggcg ccacacgtgg tacacccata tgctcacact ccctcccggc
     6901 ttggccaatt tgacgatgct gcggaaagcg cgctccgtgt ccggagtgtg gtgcagcaca
     6961 ccgatactgt acacgtgatc aaacgaagca gggcgaagtg gaagccggag gatatctccc
     7021 tgcaccacat ggacaaacgg attccggcgg ttctcttcgt aggcgcgatt cgtggcaagg
     7081 ctcaggtcca ggcccacgac ctccgagggc gcttcgtccg cgatgacctg aaggtaccgt
     7141 cccatgccgc agccggcctc gaagaaaagc tgggttccca gcgatccggg cgttgttccc
     7201 gtgcggcggc aaaagagcgc acggttctca gcgtgatcgg tcacgcggaa ccggagccat
     7261 tgaaagccga agctccgttt cgtttcggag tggagcgcct ggaagtcccc atccgccggt
     7321 ctgcgatacc ggaaggcttg cgcatactgc cgaaagaagt ccggattgtc ttgctcgaca
     7381 tcggggttga agcgtggcac gccgtcgacg atggggaact caacgcggca agcatcgcag
     7441 cggagaattc cctgtacgat ttcgactccg tagcactcga cgcacgggcg cactcgaccg
     7501 ggagcctctt ccatgctctc cgaatgccac gaacaatacg acgagcagcg tgtcccacaa
     7561 tcggccggcc ggtgcgcggg ctcggtttcg agcacggtca gatcgagcgg agagcggccg
     7621 cagaagcaac aggccagaag atctcgaagt cggtgtttca aacctcactc gtcggcgccc
     7681 aaatggcgca gcgcattctc cgatgctagc actgagctgc cgtgaagtag gatgaaaggt
     7741 accctcggat ggcacaggcc acgacagcag acggtcagtc ccggcgctcg gatcggctgg
     7801 tctccggcgt cctgtggaac tgggccggga tcggggtgca actcttcacc ggtgtaatcg
     7861 tctcgcctta catcatccgg aaacttggca cggagcgata cggaatttgg gccctggtct
     7921 tctcgctggc cagctactac ggcttcatcg atctcggctt ccggtccgca gtggttcggt
     7981 tcagcgccca ttattacacc cggcgcgaga acccgaaagt caacgagctg atcaatacaa
     8041 cgttttttta cttttcgctc gcagccgcga tgctggtctg tttgacactg atcctctgga
     8101 agttggcgga ccgtatcttc cgagtttcgc ctgcgcttcg ggatgaattt tcatggctcg
     8161 ttctgatcgt cggcatcagc atatccgtcg gactgaacat gagtgttttt tccggagtgg
     8221 tggagggttt gcagcgcttc gacatctcaa accgcatccg gatcgttgtc ttcgggttgc
     8281 gtggagcagg atggttcctg ctactcatgg caggttatgg gttggtggcg atggggtggt
     8341 ggagcctggc caccagcgtg gtcttctttt tcctctacct gctttatctg cgaaggatct
     8401 ttccggcgtt ggaattctcg cggcagtttg tgacgctgtc catgttcaga gagacggtga
     8461 gctatgggct ccacacattt ctcgcgagta tcggcaccca gtccctcgat cagacgccct
     8521 ccctgctaat cggctacttt cggagagcgg cggatgtcgg gtattacaat ttccccctcc
     8581 gcgtgatcca atacgtgtcg caagcaatta cgaatgtggg ggtcgtcgcg ctccctcatg
     8641 ctgcatcgct tgcggcagag ggacgactcg acaaggtcgc gaacctgggg atctatgcca
     8701 accggtattg tctatcgtta ttcatgccgt tggcggtatt cctgactgtc tacggaaccg
     8761 aacttttctc gatatgggta acacccgagt tcgcccggaa cgctgtctat ctgctacccc
     8821 cgttgcttgc gggcttcggg cttggccaag ccgcgcagtt ctgcaccgga tcgattctct
     8881 tcggcctggg agaacagaag atgtatgccc gcgcgattgt tggagaggcg gccgcaagca
     8941 tcgtgttcat ggtggcggtg atcccgcgct acggcgtcta tggcgcagca gtggtcaccg
     9001 caagtttgat gacagtcact cgaggatgca tcggtccgtg gatcttgtgc ctgcgcctcg
     9061 gtttttcttt ccagagatac atgcagtcga tcctggcgcc gcctctggct gcctccgtcc
     9121 ctgcatggct agcgctctgg gggctcaagc gctggggcgt cttggggaag aactggagtg
     9181 agctcatcct ggccggcggg gtcgtttgcg cgctgtacgg agtgactgcc ctctacctct
     9241 gtgtgagcaa agaccacagg aggctgctct ttaactgggc aggacagcgt ctgggccggc
     9301 gagatggtcc gcagcggcat tcgtgatccg gcgggccgca gggagcctgc ccgacgaggc
     9361 gcccgctgat gcagacctga gatagttcag ccatcttccg agcgcactca aactcaggag
     9421 cgcctcaagc ggcgcatcgt cgccactccg aaaccactga agaatcgtct cgacttcgtg
     9481 tttccgaaaa agcggaactc catccaacaa gcagagccct tgctcgaggg cctcagaacc
     9541 gagcggcgtt cgaaacagtg gtgagagcgg tggccacgag ccctcgtcga gatatactgc
     9601 accagaagct gccgagccat ctgacacggg ccgcaacttc ctccaggcag ttcgcgcgag
     9661 aggataccac gcctgattgc ggaaggtggc tcgccaatcc cggcttaccc tccccgacga
     9721 tctgccggtg ttggagtcgg gcagtcccgc gaccggatgg ccagcgccgg ccaacgccgc
     9781 gcgaaaagcc cgagcttcgt aatacgcatc aggcgggctc aatcgcgccc acgaccaaag
     9841 cgcccggtgg aaaatcggcg aattgacatc gagttcctcg atccaaccca gcaaaagcgg
     9901 atagtggcac tggcggctga tgaaagcgat caagcgattt tgcagcgtta gatccaaatc
     9961 gccggcgacc gcgggctcca tgctgttcgc caggtccttc agacgctgtg ctgcaatttg
    10021 ccttgcttcc ttggtgaggg attggagtac gaattcggac gtcgcgcggt gcgagcggcc
    10081 cgaccgtttc tcggcgaaaa tcactgcaga attaccaaga acgtcgtgga gcggcagata
    10141 cgggctcaca tacttctggc gggggaaagc gaagtagccc tttagaacgg tatcgtacag
    10201 gaaggcgttg cagacttcgt gaataccctc tttcctccat tcgacgccca atccaaggtg
    10261 gtgcggcgtg agcggatccc acatgcaggc ggtaagcagg aaactcgact cgagcaaagt
    10321 gacgtagtgc tccggcggcc gcggccagtg gaggaacttg gctccacaag ctccagcgat
    10381 gtcgcgcgct atccggcact cacgattctc acgagtcgtc acggtcattg ctttccggtc
    10441 cgagcccgct tgagacaacg tgagccgcga atcgagacca ccgctcaaca ggagatagga
    10501 atcccgttcg acccaggttt ccgcgaccgc gcggcgcaga gcggcgagca gggttccagg
    10561 cgggtcttcg gcaatgcgat gggacgataa ctcaggatca agcggcattt cgaagcgccc
    10621 gcgcacgacg atgccgtcct gccgaaactg gataacctca ccgggctgaa actgctgggc
    10681 ttgtgcgatc gaggtatcgg ttgtgtaaag cgtcccgagt gtgagcgctt cgaggatcgc
    10741 gggccagttg ggcgacaact ccccagtacg gttcagggcg agtgtggaca atttgtcaga
    10801 gacgaggctc cctccacccg tggggacgaa aaaaaccggt cgcgtggcga gagggtcgac
    10861 gaacgccgta agccgggcgg gaagtccggc aaggatctcg aatccgcgaa aactgcccca
    10921 gcggccatcc aaccactgat cgagcggttc gttctccctc gggtagcgat caggctgtcc
    10981 ttcgatcagg aggaatcgcc acgcctcccg atagatcctc acctgccggc tggctgcggg
    11041 gtacgtccaa gatgcgcgca ccgtccctgc agagaaagag tccgcggggc cacaatcccc
    11101 aaggtccacg agcttaggcg atggaccagc gaggccattt tctgacgatc cagattctat
    11161 gagaaaacct gccacgagat gacgagaggt tcccttgcga ttgtggcaca gggcggccgc
    11221 attatccaag aaacgcgcca tgctatcatc ggcgcaagcg agggcagatg gctactgggc
    11281 aactggaact ggaactgtcg gacgtgcgcg gcgcgaacct ggacgacacc gtccgaatcg
    11341 aattgtttga cgagcagggc aactgcgact atcgaaatcg cgagcaggtc gctcgccaga
    11401 tcatactcac aggaatcgaa agctcgccgc tgaccttcta ccgcgtggcg gtgaccccga
    11461 cacggtaccg gatacggcaa gcgttcgtcg ctgtcaagga gggaaagaca gtcactttga
    11521 atttatcctt tccggtcgaa ccacgaaagg tcaaggacat ccaggcgcgt ccatttcccg
    11581 atcttgcggt cagcctgcag gcagttctgg agatctccga catacctgcg taccgcgacg
    11641 acaagggcaa tccgcttcag ggtgaggaac tttacgaggc cctaaaaccg cgcgacagag
    11701 catgcctgtt gaacctctac gcgaaagcct cgaacacggt gctcggcgac ggaaaatcct
    11761 gcttcgacca cttcggcggg ctgaccctcc ttcaccagga tcggtttttc gcccggacac
    11821 acggagcgct cgaggaggag gcagcggtat caccgttgtt tcgagaggag tccggcgcgc
    11881 tccacgaacc gccggcgggc tataccctcg gacgcagctt caaaacccgc gacgagcacg
    11941 gaaaccttca gctcacattc ttccgcaggg gcgacacggg agacgactat ctcgttgacg
    12001 tcgatatcga cgaggcaaac ggcatcgagc acatcttcga agtggttcgg aaccaggtga
    12061 cggggcgaac gaatccgtac gacgtgcgcg agattctcat cgcttcgcag gggcttgatc
    12121 ccggctattc ttttgtgttc gggtaacaga tttggacgaa gcgggcttgg aagcctcact
    12181 gcccgggaag acaatttcgt aacccttttc cttccactcg gcaatggagc agaagccgat
    12241 cgtcttgtaa ccgttgctct gaaggatggc cgcggcacgc gcggctctcg agccgcgatt
    12301 tcaagccgtg acgatgagca cgttcttcgg gatctccggc agtctcacgc cgagttggct
    12361 cagcgggatg ttcacgtagc ccttcattga acccagcgcc ttaatttcgg cgggctccct
    12421 cacgtcgaga aagaagaact tgaccttctt gtcaatcaag gtcttcagtt cctcggcgga
    12481 gatcgccttg ggagcctgct gtgcccgcag gggcggcggt acggcgaagc cgacgagggc
    12541 aagcatgaca gagaataagg gcctgatccc gacatccata tcttcacttc agcatatccg
    12601 gggaccttcc gcctagcgct tcagcgggtc ctcgacccag ggtggagtgg cattgatgag
    12661 gcgcccaagc tccggctcga gttcgtccat cttctgtttc atgcgccagg ccacctcccg
    12721 ataactgaag tggcccttca cgccgcttcg gagccgggag atgtactcgg cttcggcgaa
    12781 atccatctta aacaggaatc gtgaacaagc gccgaacggg atcaggtagt gcgaaccagg
    12841 ctgagggagt gaacgccgga cttcaagggc ctttcgcagc gccgcctcgt aagtttcccg
    12901 cagtccggcg tccacaatcg ccttcggcgt ctcaaaaccg aggttgccgt tatacgtctg
    12961 ccggtactgg tgacagcggc ggtgacggtg gaggtcacgg taagctccga tgtctatgat
    13021 catgtcgaac gcgtacaggc cgccttggaa ctcgcgcagc agctcgtcgc gccgagttcg
    13081 ggagcgcacg gcgaggtcga ttacttcgcg cctgcgctcc gcgctccagg agcaagacag
    13141 ctcgtacagc tcgcggaacg ggcggtcggt cacgggataa agcaaggttg cgacgatgtc
    13201 ggccgaggtg tcggagggtt tcagaagatc caccggctcc ggcgtgactc cggcggcggg
    13261 cggcaggttt tgatccaccc agagcttcag atccgcgcgc gactgcgcga gatacctatc
    13321 cgcatccaca aagcgagcca gcgtgggagc gaccggctca tgtacgacgc tatcgtccag
    13381 cgcgcaggca ggaacagagg cgcaggattc cgcgatttcg gcggcgagac tgcgcaactc
    13441 ctcatactcg gacgccccga gccgccggat ctgcttttcc agcgtccgga tgctggttac
    13501 ctgcccgaca ttggtgggca cgcccagaaa tagcagatgg cgcgcgacgt caaacgctcg
    13561 ggcggcaatg ttgcgctcgt aagcatccgg tttcatgctt tcggggcgtg ggagctgacc
    13621 tgcgagatag tcgcggacgg cagcgtgaat ccggctgtat gcacccagaa gcgtctccgc
    13681 ggccgtccgg tagacggcgg cgtcggcttc tccgaactcc tgtggcgtga tgaaaccggc
    13741 cgtcgaaaaa tcctggtaac gggaggactt ggcctgaccg tcccagagcg gctcgtcctc
    13801 gatttctgtg gctgcgatct cagagattcc ctcgaagcag agaacaacgt ggccgaggtc
    13861 tgctatggaa gcgtggccgt actggaaata gaagtgttcc aggaacttct gggagtcgtg
    13921 tgtgcgcacc cattccaggg actggcggat cgagtcgggc gaccggctgt agcgcgccag
    13981 agcgtacgca catttctcgg gcggcattgg agcaacggtc agcacccgcc tgggttggag
    14041 ctccctcata tcgggctatg atagcggatt ccggtttgat gaaagtcaga attcagaaac
    14101 tgcgtccgga cgccttcctg cccgagtacg cacacggtcc tgatgaagac gcgggcatgg
    14161 atctccgagc cgtcgaagac gttgtgctgg agcccggcgt gccgcgggcc gtgccaacag
    14221 gattggcgat cgaactgcag cccgggtacg aggcccaggt acgtccacga agcggcctcg
    14281 cgctgaagca cggcattaca cttccgaacg cgccggcgac catcgaccca ggctatcggg
    14341 gcgagatcaa ggtgatcctg ctgaaccttg ggcgagagtc gtatcgcgtc catgttggcg
    14401 accggatcgc gcagatggtg atcgcgcgct acgagcgtat cgagtgggaa gaggggatgc
    14461 taaacaactc ccgtcgagga accggcggct tcggctcatc ggggcggtag cggagagcag
    14521 tagcgggagg tacacggaac atgtggtgca gattgcgtac gtcactttgc cttgtggttc
    14581 ttgtgtctca accatttctg gcagtagaga agcccggagt cgtgtacaag attttccagt
    14641 ttccagccaa ccagatcccg cgcgtggacg gccggacgga cgactgggac atggtgccgg
    14701 cggattacgt gatcgggact gatcagctca cagactccaa aggcaacctg aaacacgatc
    14761 cgagagacat cgatgtgaag gtccgcgtcg gatgggtgaa ggggttgaac cgtctctact
    14821 tcctatacga ggcttatgac aacttctggg cgttcgaccg gccggacgaa cacaacgatc
    14881 tgttcgaggt agtggtagac ggcgatcttt ccgggggccc attgatcgcg gaccttcacc
    14941 cgcacaagga actcgaccct tggggaatct acagggccgt gcagggtgtt cacgcgcaga
    15001 attggcacgt tttcactccc gccgaaggca aagactgggt gatggtgtgg gggtgccagc
    15061 aaccctggat cagggaactg ccttacggca acgccgccta ctcctacaac ttcaagcacg
    15121 gtgagagcgg acacctgatc ctcgaattct ggcttacgcc gttcgattac gcaaactgcg
    15181 acggcggacc ggcggcgtcc gtggagtcgg tacttcgcga gaacaagttg atcggcatgg
    15241 cctgggcgat tcttgattac gacagtgaca gcgggccgta caaggcgttc tggaacctct
    15301 ctccaaagat cacgatgtac gggaacgcga gcgacctggt ggcgttccgg ctgatgccgc
    15361 tcgaaccgcg gttcaggaaa ccgattgagg cccaatggtc gttcaagatt ctcgacatgg
    15421 agcggcggct ggtggcgttc caggacttgt cggagggcga tatcacctcg tggacgtggg
    15481 acttcggtga cggcacgacg tcgaacgccc ggcatccgat tcatcagtac caacaaccgg
    15541 gacagtacat tacggtaacg ttgcgagtgg aaggaccggc gggcaaagac cggcgggcaa
    15601 aggtcttcga cgttgttctg aagtaagaat gctccacctg gatcgcactg aacttcactt
    15661 ggcaggaatg atctgcaact caccccaata ggtgatcgtg gacagccaca ggctgacgtg
    15721 ttccaggtag tagattttgg cgcccacgcg agtcatcttg atgtaatttc ccggcgaatc
    15781 ccgcgggtag aagtacgtgg aggcgccgga atgcagtgcc tccagttctc tgttctcacg
    15841 actcgcccaa tagaacttgt ctgccacccg agtgatcagg agccggctct ttcgggcctc
    15901 ttcaggtgtc agcagccgcc gctcagttcc ttcccttgac gcgtcaactc gcgagctcgg
    15961 cgccccctcc atcgcagcag tcgtggaatg aaacacttcc aggaccggca tggcgttggt
    16021 aaggtcggct ggcgtgtcgg gagagcttcc ctgcaccggg tttgtgaact ggccaaatga
    16081 cagcaaggcc aacagaaaca cgatcgcgaa tatggaagaa gcttgtctca tgttcagtgt
    16141 cctgattgga tgcggtagtg cccccctgga ccggtaggtt ttcccgtcgc tgtttcagtg
    16201 accgcttggc ctttccgcgt tgtcgagcca gtcaatctgg actcggtgtt ctggcccggt
    16261 gacctcgaac aggttcaccg tacggaacac aaagcggttt gtgcctttca ccaacggcac
    16321 gtccagctca tcctcacaat tcctccacgt accctcaccg ctcttcatct ggaaggtctt
    16381 gaagttcggc gtgaacgacg ccaagctcac ccgcgccttc tccccttcca ctcgcacccg
    16441 tgaagtgacg acattcggag tccactcgat ccaatcccgc tgtgtcgttt tcagcgggta
    16501 gggggtattg taaaaccagc tgggcgtgcc ttcccgatac cagatgttgt tgcggaagtt
    16561 ctcgtcatcc aacagaacca aagtcgagcc cagcgcagcc ggatagatgg tgaaccggtt
    16621 cgtgctggtc tcgaagctca cccaccggta catttccgcg gtcgg
//
LOCUS       DATIFQ010000444         9294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1299775, whole genome shotgun sequence.
ACCESSION   DATIFQ010000444 DATIFQ010000000
VERSION     DATIFQ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299775"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VLE22_22100"
     CDS             complement(<1..356)
                     /locus_tag="VLE22_22100"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HSB17159.1"
                     /translation="MRCFAILLLAQALAGAPSLEERIDALIDSSPGARGAFWGIRVVR
                     VDSREVLYGKNPDRFFIPASNTKLFTTALALERLGPEYRFRTVVVGPAPGPDGIVQGD
                     LTLVGGGDPTMSGREYP"
     gene            390..1106
                     /locus_tag="VLE22_22105"
     CDS             390..1106
                     /locus_tag="VLE22_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17160.1"
                     /translation="MEDSAAGPFYTASMGGRIASALLLASAGLIAQGTAPKVQVEDYP
                     VRGAAGKVAFGAEYMVHSIQGGGTTLIADDYLVVEVALYPPRFERLAVSSGQFTLRMN
                     GRKQVLFSQSPGMVSASIKYADWERRPTLVGSVGGRDGGIIIGQPPVTGRFPGDPRPD
                     QTRLPRPPKAPAENPGGVEPKDPVDPAELLAATALPEGEATGPVSGYVFFAYKGKTKK
                     IKSLELLYKGPAGEATLNLY"
     gene            complement(1087..2274)
                     /locus_tag="VLE22_22110"
     CDS             complement(1087..2274)
                     /locus_tag="VLE22_22110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB17161.1"
                     /translation="MLDVSSTRKAFSGCFLSGLLTSFLGAILPAWGYHLGAEYLTVGS
                     YFLSMHAGILASAILANWLLPEKGVRALLVVASALACLAFLGLALVPAAGPALWRTAG
                     IFLLGISSGVLTTTVLQALTAVYRHNQASTVNLTAIFFGLGCLATALLVGGAFYVYTV
                     PSILVLLALIPGFFAVYYARFPFQTSSVPELPSVRQALEDFKSPGAVLFSLLLFFQFG
                     NEWAVAGWLTLFLTQRLGVSPESSLGLLALYWFALLVGRVLVQGVLPRVRHGRFLLAS
                     GLLALLGCSILLSTNNLFGATSGILMIGGGFAAIYPLVVEKVGDRFPHYHPGFFNGIF
                     GFAITGGLLAPWTIGIYAHFWGIRMVMLVPMLGSCAVMLLLVLIWLYAKLTGVPEVST
                     NSR"
     gene            complement(2812..3003)
                     /locus_tag="VLE22_22115"
     CDS             complement(2812..3003)
                     /locus_tag="VLE22_22115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17162.1"
                     /translation="MPTGICVPLGKHHYGTIPVGAGRKELRVDEVVLRVGRTERAAEP
                     QEPSVKREVGRDRAREELG"
     gene            3052..3654
                     /locus_tag="VLE22_22120"
     CDS             3052..3654
                     /locus_tag="VLE22_22120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17163.1"
                     /translation="MSMAYHDRRMPSELRRADYHVSSNIGRTIHACRQSVIDARACLD
                     WLESQGYRRLGILGTSLGSCVAFIAAAHDERVRAGVFNHVSTYFGDVVWTGLSTRHVR
                     EGFGEAVTQDDLRRYWAVISPATYMDRLIGRDLRSLLIWARYDTTFLPEFSRQVVEAF
                     RARNIPHEVLTLPCAHYTTGQWPFNIIDGLAMCRFLYKKL"
     gene            4092..4310
                     /gene="rpmE"
                     /locus_tag="VLE22_22125"
     CDS             4092..4310
                     /gene="rpmE"
                     /locus_tag="VLE22_22125"
                     /inference="COORDINATES: protein motif:HMM:NF000612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HSB17164.1"
                     /translation="MKAGIHPAYNEVNIICACGNTFRSRSTHKGDVRVEICSKCHPFF
                     TGKQKLIDTAGRVDRFQKKYGRAKLNVS"
     gene            complement(4332..4727)
                     /locus_tag="VLE22_22130"
     CDS             complement(4332..4727)
                     /locus_tag="VLE22_22130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17165.1"
                     /translation="MRPSRTAVVLYLLIVFLSGALVGALAHRLYVIRGVENGGPARPE
                     SYRSRYIEEMRTRLNLDEGQVQLLNGILDDARTRYRAAREKIRPELDTIRQDQVGKIR
                     SMLSEGQRIEYEKLLEEREKRHRQRPTPH"
     gene            complement(4724..5092)
                     /locus_tag="VLE22_22135"
     CDS             complement(4724..5092)
                     /locus_tag="VLE22_22135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17166.1"
                     /translation="MENSSLDNGEFELNAELDRLFAAYREAIATPEAGAGFMPGLWRQ
                     IEARRTFAGNLRHWAHAFVTAAVGICLLLTFLAAYSGRQPAWPDHADDPDELAAGNVP
                     EYPRYLELAALDSGAQENHR"
     gene            complement(5098..5682)
                     /locus_tag="VLE22_22140"
     CDS             complement(5098..5682)
                     /locus_tag="VLE22_22140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB17167.1"
                     /translation="MADSELIERAQRGDDTAFGEIVSSYRNRVLGTIARLISRPDDVE
                     DVGQQVFLRLYQSLGQLRTPAVFESWLYRLTVNAAYDYLRRRRKQMETRVADLSEQQL
                     LTADTAEAVRRSDDAKAKAAVRELVQSILAGVSEEDRILLLLKEVEGLSLKELEAIYH
                     VNENTLKVRLFRARQRALKALAKVKEGAGSAAKG"
     gene            complement(5846..6019)
                     /locus_tag="VLE22_22145"
     CDS             complement(5846..6019)
                     /locus_tag="VLE22_22145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17168.1"
                     /translation="MAAKKVPSHALTARPLAVKEKLDSLYLRLLLVENLIESLETYCR
                     LEQEFDRESDQAA"
     gene            6201..8525
                     /locus_tag="VLE22_22150"
     CDS             6201..8525
                     /locus_tag="VLE22_22150"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSB17169.1"
                     /translation="MDFLEGLNPRQREAVCHSDGPLLILAGAGSGKTRVITHRIAYLI
                     AAHRVPPEAILAVTFTNKAADEMRERVLSLIGGLGFWTAPLISTFHSFSVRMLRRDGA
                     RLADLRPGFNSRFTIYDEDEQLAVVKSVFRQFGLDEEFMQYRTALSRISYAKNHQETA
                     EDLYKKPDPKLQKLASVFAEYEGRLRQANALDFDDLLLEAVRLLNYDVETLRDYNERF
                     QHVLIDEYQDTNRSQYDLIRLLTAVRQNVCVVGDEDQSIYGWRGADIRNILDFERDFP
                     DARVIRLEQNYRSTKHILAAAGAVVSHNEQRKGKSLWTDGEQGDLIGFYQAEDGEQEA
                     LFIADRARRILAQEPSDRVAILYRTNSQSRQIEEALRRYGLKYLVVGGLSFYHRAEIK
                     DALAYLKVILSPDDSVSLLRIINTPARGIGRTTTDQIERYALGKKISFWQAIGRMLDE
                     KLFPTRAEAALLGFRPLIEDLRRAAEGEPIHRVLEMVLERTGYRQTLKRDESPDSDTR
                     LENLNELLNAAAEAAERGETVAEFLDHAALVAEVDELDARAPVLLLTLHNAKGLEFPS
                     VFIAGMEEGLFPHSRSVNFPSMLEEERRLCYVGMTRARKRLFLTCARHRRRYGGGQLQ
                     QGTPSRFLAEVPPQLLENLGRRGDIPQLDLFLEQRVVRESVQRTAYTGKTYNSVEHIR
                     QFFASRNDAQTEASDRRQEPKPAAPAKAQAAGGALARKAGMRAGSTITHPKYGKGTVV
                     RREGDGDDAKITVNFPGYGLKKLVAKYAGLKTEE"
     gene            8522..8680
                     /locus_tag="VLE22_22155"
     CDS             8522..8680
                     /locus_tag="VLE22_22155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17170.1"
                     /translation="MNTKKVTDKLERKKLKRTARKKAAPKPKRASGVARGSTKRKVAK
                     MVKGQRKR"
     gene            8725..>9294
                     /locus_tag="VLE22_22160"
     CDS             8725..>9294
                     /locus_tag="VLE22_22160"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB17171.1"
                     /translation="MGQLLRTKSIEALIASSEQEGRRLRRTLGPWSLTALGIGAVIGS
                     GIFILTGTAAAGETLAFRSVLHAPVLDLLIHGPDAVSTIGRPGAGPAITLSFILTALA
                     CSFAALCYAELASMIPIAGSAYTYSYATLGEIFAWVIGWDLILEYAVSNMAVAVGFSA
                     YLNDFLESMLGFQLPKQLSAPMISGGQFTG"
BASE COUNT         1899 a         2852 c         2790 g         1753 t
ORIGIN      
        1 gggtactcgc ggcccgacat ggttggatca ccgccgccca ccaaggtcag gtctccctgg
       61 acgattccgt ccggaccggg tgccggtccc acgactacgg tgcgaaaacg gtactccggt
      121 ccgagccgtt ccagtgctag cgcggtggtg aacagtttcg tgtttgacgc agggatgaag
      181 aaccggtccg gatttttccc gtacagaacc tcccggctgt caaccctcac aacccggatt
      241 ccccagaacg cgccgcgcgc tcccggagaa gagtcgatca gcgcgtcgat ccgctcctcg
      301 agtgacggcg cgccggccag cgcctgagcc agcaacaata tcgcgaagca tcgcatgaaa
      361 gctagcatag cgaggggccg ccgccgtccg tggaagactc cgcggctggt ccgttctata
      421 ctgcgagcat gggaggccgg atcgcttcag cccttctgct ggcctcggcg ggcctaatcg
      481 cgcagggtac cgctccgaag gtccaagtcg aagattatcc ggtccgcggc gcggccggga
      541 aggtcgcttt tggggcggag tatatggtcc acagcatcca aggaggcggc acgacgttga
      601 tcgccgacga ctacctcgtc gtcgaggtcg cactgtaccc gccgcgtttc gaacggctcg
      661 ccgtatccag cggccaattc acgctccgga tgaacggcag gaaacaggtg ctgttcagcc
      721 aatctcccgg catggtgtcc gcttcaatca agtacgccga ttgggagcgc cggccgactc
      781 tcgtgggcag cgtcggcggc cgcgacgggg ggattattat cgggcagcca ccggtcactg
      841 gaaggtttcc aggggatccg cgacccgacc aaacacggct tcctcgcccg ccaaaagctc
      901 cggcggaaaa tcctggcgga gtggaaccca aggaccccgt cgacccggcg gaattactgg
      961 cggccaccgc gcttccggaa ggagaggcca cggggccggt cagcggatac gtcttcttcg
     1021 cgtacaaggg caagacaaag aagatcaagt ctctcgagtt gctttacaaa gggcccgccg
     1081 gagaggctac cttgaatttg tactgacctc gggtacgccg gtgagcttcg catagagcca
     1141 gatcaacacg agcaggagca tgaccgcgca ggaacccagc atcggcacaa gcatcaccat
     1201 acggatgccc cagaagtgcg cgtagattcc gatggtccat ggcgccagca ggccgccggt
     1261 aatggcgaac ccaaagattc cgttgaagaa tcccgggtga tagtgcggaa agcggtcgcc
     1321 gaccttctcc accaccagcg gatagatcgc cgcgaagcct ccgccgatca tcaggattcc
     1381 cgacgttgcg ccgaagaggt tgttcgtcga cagaagtatg ctgcagccga gcagagccag
     1441 gagtccgctg gccagcagaa acctgccgtg gcgcacacgc ggcagcaccc cctgtaccaa
     1501 cacccgtccg acgagcagcg cgaaccagta aagcgccagc aaaccgagcg acgattcggg
     1561 gcttacgccc aggcgctgcg tcaggaacag cgtcagccat ccggcgaccg cccactcatt
     1621 gccaaactgg aagaaaagca gaaggctgaa gagaacagcc cccggactct tgaagtcctc
     1681 gagggcctgc cggacggagg gaagctcggg gaccgagctc gtctggaacg gaaaccgtgc
     1741 atagtacacg gcaaagaagc cggggatcaa ggccaggaga accaggatgc taggtacggt
     1801 gtacacatag aaggcgcctc ccaccaaaag ggcggtcgcc agacaaccaa ggccaaaaaa
     1861 gatggcggta aggttgacgg tcgaggcctg gttatgccga tagacggccg tcagagcctg
     1921 aaggacggtc gtggtcagga cgccggagct gattccaagc aggaagatgc cggcggtccg
     1981 ccagagggcc ggccccgcag cggggacgag ggctaatccg agaaacgcaa ggcatgccag
     2041 cgccgaggct accacaagca gggcacgaac ccccttttcc ggcagcagcc aattcgccaa
     2101 gatcgccgac gcaaggatgc ccgcatgcat actgagaaag tagcttccta ccgtaaggta
     2161 ctccgcacca aggtggtacc cccacgccgg gagaatggcg cccagaaagg acgtcaaaag
     2221 acccgagagg aagcaccctg aaaaagcctt ccgggtggag gagacatcta gcatcttcgc
     2281 cggttcgccg ccggacgctt aaggaaacgt ggtccgcggc cgatcagggg tattgtatct
     2341 cgtttgccgt tcgagcattg gacgactcaa ttcaattaac ggaagggttt ccagcgtcgt
     2401 tcctatgaaa aatcggaact tgtgccggac cggtcctgta atgtcaatac attagcctct
     2461 ggaataagaa ttgctcttac gagatcgggc ctagagaggt tggacgtttt ttggggggtc
     2521 tgccttccca gagcaaactt agtagccccg catttcgcgg ggtttccttt tttcttgggc
     2581 gcagtattcc tcgcaagcct tcgtgattct gctaaactgt acgttcctgc cgctagaatg
     2641 ttcggatggt actcgcgttt gatcgattca tgggagaccc ggctcgctac tcgggatacg
     2701 aaccgtgtcg tgcggccatt cgactggggt ctcgattggc ttgatctatc tgaaaagaat
     2761 ggggatgtcc ggtccagcct tagttggtat acctcaagtg ccgtcgcgga ttcaaccgag
     2821 ttcttcgcgg gcgcggtccc gaccgacttc gcgcttgacg gaaggctcct gaggttcagc
     2881 agcccgctcc gttcgcccca cccggagaac aacctcgtcc acgcggagtt ctttcctgcc
     2941 cgctccgacc gggatcgtgc cgtagtggtg cttccccagt ggaacgcaga tgccggtggg
     3001 cacgtcggac tctgtaagtt attgaatcgt ttcggcttga ctgctctgcg aatgagcatg
     3061 gcttaccatg accggcggat gccatcggaa ttgcggcggg cggactatca cgtttccagc
     3121 aacatcggcc gcaccattca cgcatgccgg caatccgtga tcgatgcgcg tgcgtgcctc
     3181 gattggctgg agtcccaggg atacagaagg cttggcatcc tgggcacgag cctgggctcc
     3241 tgcgtcgcct ttatcgcggc agcacacgac gagcgggtgc gcgccggcgt ctttaaccat
     3301 gtttccacgt atttcggaga tgtggtctgg accggtctgt cgacgcgcca cgtccgcgaa
     3361 ggctttggcg aagccgtcac ccaggatgac ctgcgccgtt actgggcggt gatcagtccc
     3421 gccacgtata tggaccggct gatcggccgc gatctcagga gcctgctgat ctgggcaagg
     3481 tacgacacga cgttcctgcc ggagttctcc cgccaagttg tcgaggcatt ccgggcgcgc
     3541 aatatccccc acgaggtcct gacgctgccc tgcgcccatt acaccaccgg tcagtggccc
     3601 ttcaacatca tcgatgggtt ggcaatgtgc cgcttcctct acaaaaaact gtagcgatca
     3661 tcgaagatac actagatgtg gggtttcata ccctcagcag gtcgaatttc ctattgacaa
     3721 agaatttgga gccgactagt atgggggcat cactgagaaa ggaggtggtc cagcaaacat
     3781 gagttctggt agtagtaccc cgcgcgaggt ggccgacagc taggccgacc gttctcaagt
     3841 agccggcccc gtttcagtga cgaggccaaa gttcctgtgg accggtcgcc tcggcgcaag
     3901 gttttgcctc aggtagactc ctttggctac gaacccccgt ggtgtgcata cgccgccgcg
     3961 ggggttttga tttctaccca tctccgggtg ttatactgaa ggatctgagc cgtgtgcgcc
     4021 agccaaccgc cggacacggg acagtaattt ctccgccccc tcaagcagga aacgaaagga
     4081 tcggtatcgc aatgaaggca gggattcacc ccgcatacaa tgaagtcaac atcatttgcg
     4141 cttgtgggaa tacgtttcgc agccgttcga cccacaaggg agacgtccgc gtggaaatct
     4201 gttccaagtg ccacccgttc ttcacgggca agcagaaatt gatcgacacc gcgggtcgcg
     4261 ttgaccggtt ccagaagaag tacggccggg cgaagctgaa cgtttcctga gcgtaccaga
     4321 ccgaccggct gctagtgggg agttggtctc tggcggtgcc gcttctcccg ttcttcgagc
     4381 agtttctcgt actcgatccg ctgcccttcg cttagcatgg aacggatctt cccaacctgg
     4441 tcctggcgga tggtgtcgag ctcgggcctg atcttctccc gcgccgcccg gtagcgggtt
     4501 cgcgcatcgt cgagtatccc gttgagaagc tggacctgtc cctcatcgag gttgagccgg
     4561 gtccgcattt cttcaatgta tctgctccgg taactctcgg gccgagccgg cccaccgttc
     4621 tcgacccctc ggatgacgta gagacggtgc gccagggcgc cgaccagggc gccactcaga
     4681 aacacgatca ggagatagag aacgacggcc gtgcgcgacg gcctcatcga tggttctcct
     4741 gggcgccgct gtcgagtgcg gccagttcca ggtatcgcgg atactccggc acgttaccgg
     4801 cagccagttc gtcgggatcg tcggcgtggt ccggccatgc cggctgccgg ccggaatagg
     4861 ccgcgaggaa ggtcaacaac aggcagatgc cgaccgcggc tgtcacgaac gcatgtgccc
     4921 aatgccgcaa gtttccggcg aaagttcttc gcgcctcgat ctggcgccaa agccccggca
     4981 tgaatcccgc gcccgcttcc ggggtggcta tcgcttcccg gtaggccgcg aagaggcggt
     5041 ccagttccgc gtttaattca aactcaccgt tatccagcga actgttctcc atctacccta
     5101 tcctttcgcc gcgcttccgg ccccctcttt tactttcgcg agcgctttga gcgctcgctg
     5161 ccgcgcgcgg aaaagcctta ccttcagggt attctcgttc acatgataga tcgcttccag
     5221 ttccttgagc gagagaccct cgacctcctt tagcaggaga agaatccggt cttcttccga
     5281 gacgccggct aggatcgact gcacgagctc ccgaacggcg gccttcgctt ttgcgtcgtc
     5341 actgcgtctc accgcctcag cagtgtcggc ggtgagcaac tgctgctccg acagatccgc
     5401 gacccgagtc tccatctgct ttcgccgtcg ccgtaaatag tcgtaggcgg cgttcaccgt
     5461 taagcggtac agccaggact caaaaaccgc cggcgtccgt aattgcccga gcgactggta
     5521 caggcgcaag aacacctgct gcccgacatc ctcaacgtca tcgggacggc tgatgagcct
     5581 tgcaatcgtg ccgagaacgc ggttccggta agaggagaca atctcgccaa atgcagtgtc
     5641 gtcgccacgc tgcgctcgct cgatgagttc ggaatccgcc accttgccgc ctgcacggcc
     5701 gtcatccgga aggctgacct ttcccccaaa cgaaatggcg aaccggcaaa cctcaaggtt
     5761 acaaggggat agtgctaaaa cgcaaaaacc gaagggaagc ggccagcagg agaatgcgcc
     5821 cccggccggc cgatcacggc taggtttagg ccgcttggtc ggactcgcgg tcgaactcct
     5881 gttcgagccg gcagtaggtc tctaatgact cgatcaggtt ctcaacgaga agcagcctca
     5941 aatagaggga atccagcttt tccttcacag cgagtggtct ggccgtcagg gcgtgggagg
     6001 ggactttctt cgcagccatg gtatacatgt actaggagta ccataagact agcaagaccc
     6061 acccgatgtc atggtgatag gtatcttgcg gggagcgtcc tggaacattt gtaggtgtat
     6121 acacttacac tactggcagg cttaagcggc ggccaccgcc tgcgtggtag aatcggcaat
     6181 cgtcttcatt tctcagttcg atggatttcc tcgaagggct caatccacgc cagcgggagg
     6241 ctgtctgtca ctctgacggg ccactgctca tccttgccgg cgccggaagc ggaaaaacca
     6301 gggtcatcac gcatcggatt gcctacctga tcgccgctca tcgcgtgccg ccggaggcga
     6361 tcctggcggt gacttttacg aacaaggccg ctgatgagat gcgggagcgc gttttgtcgc
     6421 tgatcggcgg tctcggtttt tggaccgcgc ccctgatctc gacgtttcac tccttctctg
     6481 tcagaatgct gcggcgcgac ggcgcccgtc tggcggactt gcgtcctggt ttcaactcgc
     6541 gtttcaccat ctacgacgag gatgagcaac ttgccgtcgt taagtcagta ttccgtcagt
     6601 ttggcctcga cgaggagttc atgcagtacc gcaccgccct gtcgagaatc agctacgcca
     6661 agaaccacca ggaaaccgcc gaagacctct acaaaaagcc cgatccgaaa cttcagaaac
     6721 tcgcgtccgt tttcgcggaa tacgaaggcc ggctccggca ggccaacgct ctcgatttcg
     6781 acgaccttct tctggaagcg gtccgcctgc tcaactacga cgtggaaacc ttgcgcgact
     6841 acaatgagcg cttccagcat gtgctgatcg atgagtacca ggacaccaac cggagccaat
     6901 acgacttgat ccggctgctc acagccgtcc gccagaacgt gtgcgtcgtc ggcgacgaag
     6961 accagtcgat ctatggctgg aggggcgcgg acataaggaa cattctcgac ttcgaacgtg
     7021 attttccgga cgcccgcgtg attcgcctcg aacagaacta tcggtcgaca aaacacatcc
     7081 tcgcggcggc cggcgctgtc gtctcgcaca acgagcagcg caaggggaaa tcgctctgga
     7141 ccgacgggga acagggcgat ctgatcggct tctaccaggc cgaggacggc gaacaggaag
     7201 cgctcttcat cgccgaccgg gcacggcgca tcctcgcgca ggaaccttcc gatcgggtcg
     7261 caatcctcta ccggaccaac tcgcaatcgc ggcagattga agaggcgttg cggcgctatg
     7321 ggctcaagta tcttgtagtg ggcgggctga gtttctatca ccgggcggag atcaaagacg
     7381 ccctggccta cctgaaggtg attctctctc ccgatgactc ggttagcctt ctgcgcatca
     7441 tcaacacgcc agcgcgcgga atcggccgca ctaccaccga ccaaatcgaa cgctacgcgt
     7501 tggggaagaa aatcagcttt tggcaggcga tcggccggat gctggatgaa aagctgttcc
     7561 ccacccgtgc tgaagccgcc ttgttgggtt tccgcccgct catcgaagat ctaaggcggg
     7621 cggcggaagg cgagccgatt cacagggtcc tcgagatggt tctcgaacgt accggctacc
     7681 ggcagacgct caagagggac gagtcccctg attccgacac ccggctcgag aatttgaacg
     7741 agctactcaa tgccgccgct gaagcggcag aacgcggtga aacggtcgcc gagttcctgg
     7801 atcatgccgc gctcgtcgcc gaggtggacg aactcgatgc ccgcgcgccg gttctgctgt
     7861 tgacgctcca caacgccaaa gggctcgagt tcccctccgt ctttatcgcg ggaatggagg
     7921 aaggactttt cccgcacagc cggtcggtga acttcccgtc gatgctcgag gaagagcgcc
     7981 ggctctgcta cgtcggcatg acccgtgccc ggaagcgcct gttcctgacc tgcgcccggc
     8041 accgccgccg ctacggtgga ggccagcttc aacaggggac gccctcgcgt ttcctcgcgg
     8101 aagtgccgcc gcagcttctg gagaatctgg gacggcgcgg cgacatccca caactcgacc
     8161 tgtttctcga acagcgagtt gttcgcgaaa gcgtgcagcg gacggcgtat actggaaaaa
     8221 catataattc cgtggagcac atccggcagt tctttgcttc ccggaatgac gctcagaccg
     8281 aggcgtccga ccggcggcag gaaccgaaac cggctgctcc ggcgaaggcc caggcagcgg
     8341 gcggcgcgtt ggcgcggaaa gccgggatgc gggcgggttc tacgatcacg cacccaaagt
     8401 acggcaaagg caccgtagtc cgacgggaag gcgacggcga cgacgccaag atcacagtca
     8461 atttcccggg atacggactc aagaaacttg ttgcgaaata cgctggattg aaaacggaag
     8521 aatgaacacc aaaaaagtca ccgacaagct ggaacggaaa aagctgaagc gcacggcccg
     8581 gaagaaggct gccccgaagc cgaagcgcgc ctcgggcgtg gcccgcggct cgacgaagcg
     8641 aaaggtcgcg aagatggtca agggccagcg caagagataa gcggcggccg gaggcgggcc
     8701 ggtccgcctc ccgctttcca agtcatgggc cagcttctcc gcaccaagag catcgaggct
     8761 ctgatcgctt cctccgagca ggagggcaga cggctccggc gaacgctcgg tccgtggagc
     8821 ctcaccgctc tggggatcgg tgcggtgatt gggtcgggta ttttcatcct gacgggcacg
     8881 gcggcggcgg gggaaacgct tgcgttccgc tccgtcctcc acgcgcccgt cctcgacttg
     8941 ctgatccacg gtccggacgc cgtgtccacg atcggccgtc ccggggcggg gcccgccatc
     9001 acgctgtcat tcatcctgac cgcgctggca tgcagtttcg cggcgctctg ctacgcggaa
     9061 ctggcctcca tgatccccat cgccggcagt gcatacacct attcctatgc gacgcttggc
     9121 gagatcttcg cctgggtgat cggctgggac ctaatcctcg aatacgcagt ctccaacatg
     9181 gccgtagccg tgggcttttc agcctatctc aacgacttcc tcgaaagcat gctcggcttt
     9241 cagttgccca aacagctctc cgctccgatg atctcgggcg ggcagttcac tgga
//
LOCUS       DATIFQ010000445         5566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2066950, whole genome shotgun sequence.
ACCESSION   DATIFQ010000445 DATIFQ010000000
VERSION     DATIFQ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066950"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VLE22_22165"
     CDS             complement(<1..653)
                     /locus_tag="VLE22_22165"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17172.1"
                     /translation="MSSERTGAATPFRFGLLAAILVAVGASSCNRRPAPVQRLAIPAF
                     ENLGADKSDDWMATAFAEVLAGQLRRSGVVRPIRVATRADAAATGATHILHGYFYRRK
                     GRLVVKGSVEEVASVKTSRTLTAAGPTLLTAANTIARQIDPKAEPYGTSNEAALRALL
                     EGRAAGDPASAAAAFERAVKADPAFGAASAAWGQALTSEKRYAEAAAALREAMRRDP"
     gene            complement(643..1434)
                     /locus_tag="VLE22_22170"
     CDS             complement(643..1434)
                     /locus_tag="VLE22_22170"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17173.1"
                     /translation="MTTANLLFMVVAMAAALPVVCQTAPVAQEQTTALEPPNPPPTAK
                     PDTPLTPEKRGDVFMARKMYREAIEAYEQEPVRTPVVWNKIGIAYHQMMQLGTAKRCY
                     EKSLKLNPKYPEARNNLGAVFYAQRSYRRAIKEYNRALKLNPNSASIYSNLGTAYFAR
                     KKYKDAFKAYQQALAIDPEVFEHRSTHGVLLQERTVAERATFHYFLAKTYAQAGMNER
                     ALEYIRKSLEEGFKDRKKFIEEPEFAALQALPEFQELMTKDFRVL"
     gene            complement(1451..3958)
                     /locus_tag="VLE22_22175"
     CDS             complement(1451..3958)
                     /locus_tag="VLE22_22175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HSB17174.1"
                     /translation="MGIRFQIPRKALLVRFLMHPAGKILLTLTVLFGTLSLGTFTFYY
                     LRYSRLIDRKLNIGPFQNTSMIFAGPPVVSAGDSLTADEVVNLLRRSGYSESRTNPLG
                     WYHLRPDAVEIFPGPDSYFDQEAGVIRFSGSRVSQIISLRDNTERRQYMLEPELITNL
                     FDKNREKRRLVAFQDIPKELVNAVVSAEDKRFFQHSGFDPLRIIKAAYVDLKEGRKEQ
                     GASTLSMQLARSLWLDQRKTWRRKAAEVLITLHLEQKLTKEQIFEYYANQVDLGRKGS
                     FAIRGFGEAAQAYFAKDIRDLTVAEAATLAGLIQRPSFTNPIRWPDRARGRRNVVLAL
                     MRENGYISDQDYAAAASSPLTVVRGGGGSSDAPYFVDLVNDELQERFQDHDFQTSSYR
                     IYTTLDLNLQRDANEAVRIGLQEVDQQLQRRRKRNPNLPEAQVALVALDPLTGEIKAL
                     IGGRNYGVSQLDRALAKRQPGSAFKPFVYAAALEAALQGRLPDFSPLTTVVDEPTTFW
                     FDDKPYTPGNFKEEYRGTVTVRQAISKSMNVPTVKVAEMAGYDSVVDVARRAGMNLRI
                     QPTPAVALGAYEVTPIEVAGAYTVFANQGVHVKPYWIRLIRDDKGRIMHNYTPQTEAV
                     LDPRVAYEMVDLLQEVMRSGTAAGVRGRGFTLPAAGKTGTSRDGWFAGFTSKLICVVW
                     VGFDDNQELGLEGARSALPIWTEFMKRAHQHREYRNARSFEPPDGIVMVEIDPLTGGL
                     ATSACPKPQLQAFLAGTQPVETCRLHGGGVLTATQISGWETPSAGGKTGPVVQARVQQ
                     RQSAQAGQSVSAAKPQEQSKPKEGKKSLFRRFLDVFK"
     gene            complement(4042..4434)
                     /locus_tag="VLE22_22180"
     CDS             complement(4042..4434)
                     /locus_tag="VLE22_22180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17175.1"
                     /translation="MTRRGAILALGDRWNVAIEARHWRTAGGVTVLAALGLFAARLVP
                     VYVSNFRLQQFLEETAQRRSTIEAPDDLLRVRVVNEAARLGLPVRADQVRVRRTPGKI
                     RIEVLYIVPVDLPLYTVDLHFRPTADGG"
     gene            complement(4431..>5566)
                     /locus_tag="VLE22_22185"
     CDS             complement(4431..>5566)
                     /locus_tag="VLE22_22185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17176.1"
                     /translation="AFIRSPGAGVNELQFLSVSGDLRTVGKPRRLILPQRAINYPAWT
                     EDGREVVFSAGFAGEKGLWRVAISGAGEPRPLALPAEKAVHPQISRDGRRLVYAAVSV
                     AYNLWRMEISPRENTLPHRITPSTQWDTDPQYSPDGRRIAFTSLRSADGEIWTCDPDG
                     SNAVRVTSLPFSHASRWFPDGRRIAFQSNKEGDFDVWVIDAEGGAPKRLTTGPANEYY
                     PSVSRDGKSIYFASNRTGRYEIWRMPSDGGEAVQVTRQGGAIPFESADGKDVYYLKVM
                     TTPSELWRAPVGGGEEAKVLDSVTQRAFAVRQDGIYFLRPLEPLGGVSLQFFRFASRT
                     SAEIAKIERRTAYGLTVAPDGRSVIYPLQEQSNADLMLVDNFR"
BASE COUNT          977 a         1765 c         1749 g         1075 t
ORIGIN      
        1 tccggatcgc gtctcatcgc ttcgcgcaag gcggcagccg cttcggcgta ccgtttctca
       61 gaggtcagcg cttgacccca ggcggcagac gcagccccga acgcaggatc ggcctttacc
      121 gcacgctcga aggcggcggc cgccgacgcc ggatcacccg cggcgcgccc ttctaatagt
      181 gctcgcagcg ctgcctcgtt agacgtgccg tagggttcgg ctttcggatc gatctgtcgc
      241 gcgatggtgt ttgccgcggt cagcaaggtc ggtcccgccg ccgtgagtgt cctcgacgtc
      301 tttacggagg cgacttcttc caccgatcct ttcactacca gacgcccctt tcggcggtag
      361 aagtaaccat gcaggatgtg cgtcgccccc gtcgccgctg cgtccgcccg cgtcgccacg
      421 cgtatgggcc ggacgacgcc gctgcgccgc aattgcccgg ccagaacttc ggcgaaagcc
      481 gtggccatcc agtcgtccga cttgtcagcg ccgaggttct cgaaggcagg gatggcgagc
      541 cgttggacgg gtgcgggtct ccggttgcac gacgaggccc caaccgcgac taatatggcg
      601 gcgagaagcc cgaaccggaa tggagtagcc gctcctgtcc tctcagagga cacgaaagtc
      661 cttggtcatc aactcctgga attcggggag cgcctggagg gctgcaaact ccggttcttc
      721 gatgaacttc ttgcggtcct tgaagccctc ttcaagcgac ttgcggatgt actccagtgc
      781 gcgctcattc attcccgcct gcgcgtacgt cttggcgagg aagtagtgga atgtcgcgcg
      841 ctcggcgacc gtccgctcct ggagcagaac gccgtgggtg ctgcgatgct cgaacacttc
      901 cgggtcgatc gctaaggcct gctggtaagc cttgaatgcg tccttgtact ttttccgcgc
      961 gaagtacgcc gtgccgagat tgctgtaaat ggaggccgaa ttcgggttca atttcagcgc
     1021 ccggttgtat tccttgatcg cacggcggta acttcgctgc gcgtaaaaca ccgctcccag
     1081 gttgtttcgg gcttccgggt acttcgggtt cagcttgagc gacttttcgt agcagcgctt
     1141 ggccgtgccg agctgcatca tctggtggta cgcaatgccg atcttgttcc agacaacagg
     1201 cgtccgaaca ggttcctgct cataggcctc aatcgcctcc cggtacatct tgcgggccat
     1261 gaacacgtcg ccgcgttttt ccggcgtcaa aggggtatcg ggtttcgcag tgggaggagg
     1321 atttgggggc tcgagcgcgg tggtctgttc ctgagcgaca ggtgctgtct ggcataccac
     1381 cggaagcgcc gccgccattg ccacgaccat gaacaacaga tttgcggtcg tcacggccca
     1441 cctcctggct ttatttaaac acatccagga atcggcggaa cagactcttt tttccttctt
     1501 ttggcttgga ttgttcctgc ggcttcgccg cggacacgga ctggccggcc tgggctgact
     1561 ggcgttgttg gaccctggcc tggacgaccg gtccggtctt acccccagcc gagggggtct
     1621 cccaacccga gatctgcgtc gcggtgagca ccccgccacc atggaggcgg cacgtttcta
     1681 ccggctgggt acccgccaga aacgcctgca actgcggctt cgggcaggct gaggtcgcca
     1741 ggccgccggt cagcggatca atctcgacca tcacgatgcc gtcgggcggt tcgaaggacc
     1801 gggcgttgcg gtactcgcga tgctgatgcg cgcgcttcat gaactccgtc cagatcggca
     1861 gcgccgagcg cgcgccttcg agaccgagct cctggttgtc gtcgaagccg acccacacca
     1921 cgcagatgag tttcgaagtg aagccagcga accagccgtc gcgcgaggtg cccgtcttgc
     1981 cggccgcggg gagcgtgaag ccgcggccgc gtacgcccgc cgcggtgccg ctgcgcataa
     2041 cctcttggag caggtccacc atctcgtagg ccacccgcgg atcgagcacg gcctctgtct
     2101 gcggcgtgta gttgtgcatg atccggccct tgtcgtcacg gatcagcctg atccagtatg
     2161 gcttcacatg cacgccctgg tttgcgaaca ccgtgtaagc tccggcgacc tcgatcggcg
     2221 tgacctcata ggccccgagg gctaccgccg gcgtcggctg gatgcgcagg ttcatgccgg
     2281 cccgccgcgc gacgtcgacc acggagtcat acccggccat ctcggccact ttcaccgtcg
     2341 gcacgttcat cgatttcgag atcgcttgcc gaaccgtgac cgtgccccgg tactcctcct
     2401 tgaagttccc gggcgtgtac ggtttgtcgt cgaaccagaa cgtggtcggc tcgtcaacca
     2461 ccgtggtcaa ggggctgaaa tccggtaacc tgccctggag ggcggcctcg agcgcggccg
     2521 cgtacacgaa aggcttgaag gcggaaccgg gctggcgctt ggcgagcgcc cggtccaact
     2581 ggctgactcc gtaattcctg ccgccgatca gcgccttgat ctcgccggtc agcggatcta
     2641 gggccacaag cgccacttgt gcctccggca aattcgggtt tctcttacgc cgccgctgaa
     2701 gttgctggtc cacctcctgc aaaccgatcc gcaccgcctc gttggcgtcg cgctgaaggt
     2761 tgaggtcaag ggtcgtatag atccggtagg agctggtctg gaagtcgtgg tcctggaaac
     2821 gttcctgcaa ttcgtcgttc accaggtcca cgaaatatgg cgcatcggac gatccaccgc
     2881 cgccccggac caccgtgagc ggggatgagg ctgctgccgc gtaatcctgg tcactgatgt
     2941 agccgttctc ccgcatgagc gccagaacca cgttacggcg gccccgtgcg cggtccggcc
     3001 agcgaatcgg gttggtgaag ctcgggcgct ggatgagccc cgccagggtg gccgcctcgg
     3061 ccaccgtcag atcacggatg tccttggcaa agtaagcctg cgccgcttcc ccgaagccgc
     3121 gaatggcgaa gctgcccttc cggccaaggt cgacctggtt ggcgtagtac tcgaagatct
     3181 gctccttggt gagcttttgc tcgaggtgca gggtaatcag cacctcggcc gccttgcggc
     3241 gccacgtctt tcgctggtcg agccacaggc tgcgcgcgag ctgcatgctc agcgtcgagg
     3301 cgccctgttc tttccggccc tccttgagat caacgtaggc ggccttgata atccgcaggg
     3361 ggtcgaagcc ggaatgctgg aagaaccgct tgtcctcggc cgaaaccact gcgttcacaa
     3421 gttccttggg tatgtcctga aaggcgacca ggcggcgctt ctcgcggttt ttgtcgaaca
     3481 ggttcgtgat cagttcgggc tccagcatgt actgcctccg ctcggtgttg tcccggagcg
     3541 agatgatttg tgagacgcgg ctgcccgaga atcgaatcac accggcttcc tggtcgaagt
     3601 acgaatccgg accgggaaag atctctacgg cgtccggccg gaggtgatac cagccaagcg
     3661 ggttcgtacg gctctccgaa tagccgctgc ggcggagcag gttgactacc tcgtcggccg
     3721 tgagcgaatc gccagccgaa acgacgggcg gtccggcgaa gatcatcgag gtgttttgga
     3781 aaggcccgat attgagcttc cggtcgatga ggcgggagta ccgaaggtaa tagaacgtga
     3841 acgtccccaa cgagagcgtc ccaaacaaga ccgtgagcgt cagaaggatc ttgccggcgg
     3901 ggtgcatcag gaagcggacc aggagggcct tgcggggaat ctgaaatcgt atgcccaaac
     3961 gtgttcgcct cgattgatat tttctcagaa cgcgcggcgg atttcagact ccgggcgcgc
     4021 ggttcccgcc gcggcgggaa gctaaccgcc gtcggcagtc ggccggaagt gcaagtcgac
     4081 cgtgtaaagc ggaagatcca ccggcactat gtaaaggacc tcgatgcgaa tcttgccggg
     4141 cgtgcggcgg actcggacct gatctgccct gaccggcagg ccgagccgcg ccgcctcgtt
     4201 gaccacccga acacggagaa gatcgtcagg cgcttcgatc gtggacctgc gttgggcggt
     4261 ctcttcgagg aactgctgca aacgaaagtt gctgacgtac accggcacaa gccgcgcggc
     4321 gaacaacccg agcgccgcca gcaccgtcac cccgcccgcc gtccgccagt gccgcgcttc
     4381 gatggccacg ttccaacgat ctcccagagc gagtatagcg ccccggcgcg tcacctgaaa
     4441 ttatccacca gcatcagatc ggcgttggat tgctcttgca gcgggtagat aacggaccgg
     4501 ccgtcgggcg ccacggtgag cccgtaggca gtgcgtctct caattttggc gatctccgca
     4561 ctggtgcggc tggcaaaacg gaagaactga agagaaacgc cgccaagtgg ttccaacggt
     4621 cggagaaagt agattccgtc ctgccggacg gcgaaagccc gctgcgtgac agaatcgaga
     4681 accttggcct cttcaccacc cccgacgggt gccctccaga gttccgacgg ggtggtcatc
     4741 actttcaggt agtagacatc cttgccatcc gcggattcga aggggatggc cccgccttgc
     4801 ctcgtcacct gcaccgcctc tccgccgtcc gacggcatcc tccagatctc ataccggccc
     4861 gttctgtttg acgcgaaata gatactctta ccgtcccggg aaacgctggg gtaatactcg
     4921 tttgccgggc cggtggtaag ccgcttcggc gcgccgcctt ccgcatcgat gacccacaca
     4981 tcgaagtcgc cctctttgtt ggactggaag gcgatgcggc ggccgtccgg gaaccaccgg
     5041 gaggcatggc tgaacgggag cgaggtcacc cggactgcgt tggagccgtc ggggtcgcat
     5101 gtccaaatct cgccgtctgc cgagcgaagg gaggtgaatg cgattcgtct cccgtccgga
     5161 gaatactgtg ggtcagtgtc ccactgagtg gaaggcgtga tccggtgggg aagagtgttc
     5221 tcgcgagggg atatctccat ccgccacagg ttgtaagcaa cgctcaccgc ggcgtacacg
     5281 aggcggcgcc cgtccctcga gatttgaggg tggaccgctt tctcggcggg cagcgccagc
     5341 ggcctcggct ccccggcccc ggagattgcc actctccaaa gtcccttctc gccggcaaaa
     5401 cctgcagaaa acacaacctc gcggccatcc tcggtccagg ccgggtagtt gatggcgcgc
     5461 tgcggcagga tgaggcgccg tggcttcccg acagtcctca ggtcacccga aacggaaaga
     5521 aactggagct cattgacgcc tgcgccggga cttcgaatga atgcca
//
LOCUS       DATIFQ010000446         6267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2067145, whole genome shotgun sequence.
ACCESSION   DATIFQ010000446 DATIFQ010000000
VERSION     DATIFQ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067145"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VLE22_22190"
     CDS             complement(<1..456)
                     /locus_tag="VLE22_22190"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17177.1"
                     /translation="MDRRAFVASMLGAVAANAAADRIRIGFLGASHSHAWEKSVVVRG
                     SPRWELAGVCEADAEIRARFEKADVRILSERELLEDPTIRVVAVESGVPQHAGHGQLA
                     LEAGKHLHLEKPPADNLKDLNSLLATAEKKNLLVQMGYMWRYHPGINRAL"
     gene            530..958
                     /locus_tag="VLE22_22195"
     CDS             530..958
                     /locus_tag="VLE22_22195"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HSB17178.1"
                     /translation="MNYMAAKEKKPKEPMPPLKDWLPSVALGWLIPGGGHFLLKRRVR
                     GTLLLCAVALTFLFGLMMRGVLFQPKTGDLLTTIIYCGGFLGNLASGIFYLLTVWLGY
                     AQPDVAGHVHDYGTKFLVTAGLLNVLAMVDAFEIAARRKS"
     gene            958..1143
                     /locus_tag="VLE22_22200"
     CDS             958..1143
                     /locus_tag="VLE22_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17179.1"
                     /translation="MPKVNLSHFEAALLFAFFTSIVLGVVTKKTDRERLMYGLKSFGY
                     FLLALLSIGWLMRLGHG"
     gene            1152..1814
                     /locus_tag="VLE22_22205"
     CDS             1152..1814
                     /locus_tag="VLE22_22205"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HSB17180.1"
                     /translation="MLKPEEVALFAAAREKLYSAAIGDVLDSMDLHRNFLPARIRPLT
                     PDMVVVGKAAPVVVSDGIGTGDRPAGKLLEALDSLSEGDVYITNGGSSEYALWGELLS
                     TRATHLKAAGAVMNGHHRDTAGILSVGFPTFSWGAWAQDIRYRGVVVDWNVPIEVGGI
                     PVRPGDIVFGDRDGVLVVPARLAEAVFRLALEKVETENKVRDAFRRGMPAVEAWDTYR
                     VM"
     gene            complement(1833..3056)
                     /locus_tag="VLE22_22210"
     CDS             complement(1833..3056)
                     /locus_tag="VLE22_22210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17181.1"
                     /translation="MIHAFTRRRLLAMAGIAAGARAHSTAPVRAITRGPKFHWFGYYD
                     KLQFDPSGRYALGMEVDFEHRSPKANDSIKVGMVDLDDGDRWTELGESRAWNWQQGSM
                     LQWLPGSASEVIWNDRIDGRFVSHILDVKTGKKRTLESSVYSVAPDGRWAITTDFRRL
                     NDTRPGYGYAGLPDPNENVLAPKTSGIWRVDLQTGAQKLIVSVEKVLNAPNPHGDWTG
                     AKHWFNHLLISPDGSRFIFLHRWRGKKEGRSFATRMFTANREGGKLHVIDPYGKTSHF
                     IWRDPQHVLAWAWHPSHGEKFYLYEDLTDKVEVVGPEVMTVNGHCTYLPGNRWILNDT
                     YPDKERNQNVYLYEVATGRRVPLGSFHSPPEYTREWRCDTHPRSSAGGKKIIIDSAHG
                     GNGRQMYLIDIGALV"
     gene            3100..4407
                     /gene="miaB"
                     /locus_tag="VLE22_22215"
     CDS             3100..4407
                     /gene="miaB"
                     /locus_tag="VLE22_22215"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HSB17182.1"
                     /translation="MKSFYIETFGCQMNVHDSEKVIGTLVARGYRQVETPEEAELVLY
                     NTCSIRDKAEQKVFSRLSQFKRSGNGKVFGVLGCVAQQEGERIFEKAPHVGLVCGSAS
                     YNRLPELLVQIESGGRRVTGLDLDTDDTFETPLTRRDNPHRAFITIIEGCDKSCAYCV
                     VPYTRGPERSRTSASVMEEARQLALAGYTEVQLLGQNVNSYRDPSAWDFATLLARIAE
                     IPGLHRVRFTTSHPRDFGKEIVDAIDANPVLCDQVHLPVQSGSTQVLGRMQRLYTRDE
                     YMRRIEWMKSARRSIAISTDIIVGFPGETEADFEQTLSLLDEVEYDSVFSFKYSPRPN
                     TPALRLDGQIPEEEKGRRLTIVQEKQRAIQIRRNTRVVGSVQEVLVEGYNHATCQWIG
                     RTSQNRLLNFTHSNGDGKSLLGGYLPVRVTRAGPNSLAGESVL"
     gene            4439..4912
                     /locus_tag="VLE22_22220"
     CDS             4439..4912
                     /locus_tag="VLE22_22220"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HSB17183.1"
                     /translation="MKIRGLMMDPVTNMPIVILKDVNGSSILPIWVGIYEANAIALEI
                     EKVTTPRPMTHDLIRTVLTGLETGVQKVVVSELKEDTFYAVIWLEKDGRLISVDSRPS
                     DALALALRVDCPIYVEDSVLSSSKAVSAVTDKVNNEELRRWLEGLNDEDLGRYKM"
     gene            4909..5217
                     /locus_tag="VLE22_22225"
     CDS             4909..5217
                     /locus_tag="VLE22_22225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17184.1"
                     /translation="MSLPPTETLERLAAAGIQLLPTSDIATHYVFERGGFVAMVERLD
                     HGFGTIGSPGLLTEWGFAALVWRQGKPWFVAKAHEYPVKDEQVEEARRFAADLEAALR
                     "
     gene            complement(5489..>6267)
                     /locus_tag="VLE22_22230"
     CDS             complement(5489..>6267)
                     /locus_tag="VLE22_22230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17185.1"
                     /translation="MQQKDYPAAEKHFKESLKDNPNAGEVSYWLGTVLVSQRVAEKQA
                     QALFHFARASTYEGPGALSAQGRTEINKYFEKVYNSFHGSAEGIDALRQAAKTSATPP
                     EGFKIKSSYEVALEKEEELKRSNPMLALWLNVRSELIGPNGPQYWEGSVKGAGLPGGA
                     SGVTKFKGKLVSQSPAKNPKELVLAISDASTPEVTLKLDEAMAGVAEPGTEIEFEGVA
                     SEFTKDPFMLTFDVDKAKVAGWPAPVKKAPAAKKGIRKKK"
BASE COUNT         1151 a         1935 c         1882 g         1299 t
ORIGIN      
        1 cagcgcgcgg ttgatgccgg gatggtaacg ccacatgtaa cccatttgca ccaaaaggtt
       61 cttcttctct gccgtcgcga gcagcgagtt gagatccttc aaattgtccg ccggcggctt
      121 ctcgaggtgc agatgcttgc cggcttcgag tgccagctgc ccgtgcccgg catgctgtgg
      181 cacgccggac tccaccgcca ctacccggat cgtcggatct tccagcagtt cccgttcgga
      241 caggatccgc acgtcagcct tttcgaatct cgcgcggatc tccgcgtcgg cctcgcaaac
      301 tcccgcgagt tcccatcgcg gcgacccccg cacgacgacg ctcttctccc acgcatgaga
      361 atggctggct ccgaggaatc caatccgtat acgatcggcg gcggcgttcg cggccactgc
      421 gccgagcatg gaagcaacga aggcccttcg atccatggtc acgatgttaa cccaaacggc
      481 gcggttgctt gcgcgctttc cggttctgtc cagtccgcat gctaaactca tgaattatat
      541 ggctgccaaa gagaagaagc cgaaggagcc gatgcctccg ctgaaggact ggcttccctc
      601 ggtcgcgctg ggatggctca ttcccggtgg aggacacttc ctgctgaagc gtcgggtgcg
      661 aggtacgctg ctgctgtgcg cggtcgcctt gacgttcctg ttcggactga tgatgcgcgg
      721 cgtcctgttc cagccgaaga ccggtgacct gctcacgacc atcatctact gcggcggttt
      781 ccttgggaat ctcgcctccg gaattttcta tctgctgacg gtctggctcg gatacgcgca
      841 accggatgtc gccggccacg tacacgacta cggcacgaaa ttcctcgtga ccgccggcct
      901 gctgaacgtg ctggcaatgg tcgacgcttt cgaaatcgcc gcccggagga aaagctgatg
      961 ccgaaggtca atctgtcgca ctttgaggcg gccctgctgt ttgctttctt cacttccatc
     1021 gttctcggtg tggtgacgaa gaagaccgac cgcgagcgcc tgatgtacgg actcaagagc
     1081 ttcggctatt ttctactcgc tctcctctcg atcggctggc tcatgcggct gggccacggg
     1141 taatccactc tatgcttaaa ccggaagagg ttgcgctgtt cgccgcagcg cgggaaaagc
     1201 tgtactcagc ggcgatcgga gacgtcctcg attcgatgga tcttcaccgt aactttctgc
     1261 ccgcccggat tcgtccgctc acgcccgaca tggtcgttgt cggcaaggcg gcgccggtgg
     1321 tcgtgtccga cggcatcggc acaggggacc ggcccgccgg caagctgctc gaggctctgg
     1381 attcgctttc cgagggcgat gtctacatca cgaacggagg ctccagcgag tatgcgcttt
     1441 ggggcgaatt gctttccacc cgggcgaccc acttgaaggc cgccggcgcc gtcatgaacg
     1501 gccaccaccg cgacaccgcc ggaattctct cagtagggtt tccgacgttc tcctggggcg
     1561 cttgggcgca ggacatccgc tatcgaggtg ttgtggtgga ttggaacgtg ccgatcgaag
     1621 ttggcggcat ccctgttcgg cctggcgaca tcgttttcgg tgatcgagac ggcgtactcg
     1681 tggtgcccgc gcgcctggcc gaggccgttt tccgcctggc actcgagaaa gtcgagaccg
     1741 agaacaaggt tcgcgacgcc ttccgccgtg gaatgcccgc cgttgaagcc tgggatacct
     1801 accgggtaat gtagcggcgg ggagtgcttc tctcagacaa gcgcaccgat atctatgagg
     1861 tacatctgcc gcccgttgcc cccatgcgct gagtcgatga tgatcttctt tccgcctgcg
     1921 ctggaccttg gatgcgtatc gcaccgccat tctcgggtgt actcgggcgg cgagtggaaa
     1981 cttcccaaag gcacgcgccg tccggtcgcg acttcgtaga ggtacacgtt ctgattgcgc
     2041 tccttgtcgg ggtaggtatc gttgagaatc cagcggttgc cgggcaagta cgtgcagtgg
     2101 ccgttcactg tcattacctc cgggcccacc acttcgactt tgtcggtcag gtcctcgtaa
     2161 aggtagaact tctcgccgtg tgacgggtgc cacgcccacg ccagaacatg ctgcggatcc
     2221 cgccagatga agtgcgacgt ttttccgtac ggatcgatca catggagctt cccaccctcg
     2281 cggttggcag tgaacattct cgtggcgaag ctccggccct cctttttacc gcgccagcgg
     2341 tggagaaaaa tgaaccgcga gccgtccggc gagatcagca gatggttgaa ccagtgtttg
     2401 gcgccggtcc agtcgccgtg cgggtttggc gcgttcagga ccttttccac cgagacaatg
     2461 agcttctgcg ctccagtctg gaggtcaacc ctccagatgc ctgaggtctt cggtgctagg
     2521 acgttttcgt ttgggtcggg aagcccggcg taaccgtatc ccgggcgggt gtcgttgaga
     2581 cgccggaagt cggttgtgat cgcccagcgt ccgtccggcg cgacggaata cacagacgac
     2641 tccaacgtcc gcttctttcc cgtcttcacg tcgagaatgt gcgacacgaa ccgcccatct
     2701 atccggtcgt tccagatcac ctcgctcgcc gaccccggca accattgcag catcgagccc
     2761 tgctgccagt tccaggcgcg gctttcgccc agttcagtcc accggtcccc gtcgtcgaga
     2821 tcgaccatcc cgaccttgat gctgtcgttg gccttcggcg agcggtgctc gaaatcgacc
     2881 tccattccaa gtgcgtaccg gcccgacgga tcgaactgga gcttatcgta gtaaccgaac
     2941 cagtggaatt tcggcccccg cgtgatggcc ctcacagggg ccgtgctatg cgcccgggct
     3001 cccgcggcga tacctgccat cgcgaggaga cggcggcgtg tgaatgcgtg gatcatggct
     3061 gcctcccccg agccactata ataaaaggaa acccctccaa tgaagagttt ttacatcgag
     3121 acattcggct gccagatgaa cgtgcatgat tccgagaagg tgatcggcac gctcgtcgcg
     3181 cggggctacc gtcaggtgga gactcccgag gaggcggaac ttgtccttta caacacttgc
     3241 agcatccgtg acaaggccga gcagaaggtc ttcagccggc tttcacaatt caagcgaagc
     3301 gggaacggca aggttttcgg cgtcctcgga tgcgtcgcac agcaggaggg ggagcgtatt
     3361 tttgagaaag cgccgcacgt agggcttgtg tgtggttccg ccagctataa ccggctgcct
     3421 gagctgttgg tgcagatcga gagcggcggc cggagggtca ccgggctgga cctcgacacc
     3481 gatgacactt tcgagacacc gctaacccgc cgcgacaacc cccaccgcgc cttcatcacc
     3541 atcatcgaag gctgtgacaa gtcctgcgcg tactgcgtcg tgccctacac ccgcggcccc
     3601 gagcggagcc gcaccagcgc ctcggtgatg gaagaggcgc ggcaactcgc cctggcgggc
     3661 tacaccgagg tccagcttct cgggcagaac gtcaatagct atcgggaccc ctcggcctgg
     3721 gacttcgcga cgcttctcgc tcgcatcgcc gagattccgg ggttgcaccg cgttcgcttc
     3781 acaacctcgc atccccgcga cttcggcaag gagatcgttg acgcaatcga cgcaaatccg
     3841 gttctgtgtg accaggttca tttgcctgtg cagtccggct cgacgcaggt tctcggccgg
     3901 atgcaacggc tctatactcg cgacgagtac atgcgccgca tcgagtggat gaagagcgcc
     3961 cggcggagta tcgccatctc caccgatatc atcgtgggct tccccgggga aaccgaggcg
     4021 gactttgagc agacgctctc cctcctggac gaagtcgagt acgattccgt tttcagcttc
     4081 aagtattcgc cgcgcccgaa cactccggct ctgcgactcg acggccagat ccccgaggag
     4141 gagaagggaa ggcgtctgac catcgtccag gaaaaacagc gggccatcca gatccgccgg
     4201 aacacgcgcg ttgtcggttc ggtgcaggag gtgctcgttg agggctacaa tcacgccacg
     4261 tgccagtgga tcgggcgtac ctcacagaac cgtttgctca atttcactca ctcgaacggg
     4321 gacggtaagt cgctcctggg cggctacctc ccggtgcgag tgactcgcgc cggtcccaac
     4381 tcgctcgccg gagaaagcgt gctctaatga gaagaggggg aacgccgtgg aagttgagat
     4441 gaagatccgg gggctaatga tggatcccgt cacgaatatg ccgatcgtga ttctcaagga
     4501 cgtcaacggc agctcgatcc tacccatctg ggtcggcatc tacgaggcaa acgccatcgc
     4561 actcgagatc gaaaaggtca cgacaccacg ccccatgacg cacgacctga tccgcaccgt
     4621 gctcacgggg ctcgaaacgg gcgtacaaaa ggtggtcgta agcgaactga aagaagacac
     4681 cttctacgct gtgatctggc ttgaaaagga tggccggttg atcagtgtcg actcccgccc
     4741 cagtgacgcg ttggcactag cgcttcgtgt ggactgtccc atctacgtcg aggattcagt
     4801 tctcagttct tcgaaagcgg tgagcgccgt gaccgacaag gtgaacaacg aagagttgcg
     4861 ccgctggctt gaggggttga acgacgagga cctgggccgc tataagatgt gagtcttcca
     4921 cctaccgaga cgctcgaacg gctggcggcg gccggcattc agttgttacc cacctcagac
     4981 atcgcgacgc attacgtctt cgagcgcggc ggattcgtcg ccatggtaga gcgcttggat
     5041 catggattcg gtacgatcgg cagccccggc ttgctcaccg agtggggatt tgcggcgttg
     5101 gtctggaggc aggggaaacc ctggttcgtc gcgaaagctc acgagtatcc tgtcaaggat
     5161 gaacaggttg aagaggcccg gcgctttgcg gccgaccttg aagccgccct ccgctaacga
     5221 tttccggttc ttctcgtcat agacggtatg aatggaggcg ccctcatacg ctggcggcag
     5281 ggactgatag ggcttgggct gggcgtaatg acaattctgg cgattcaata cgaccggcct
     5341 aactccctgc tacctgaacc ggcccgccgg tttgtgcagt actaccacca tttgggagaa
     5401 accgattgga aagtgcccta ctgggagagg gttgcgatca gcctggtcct ggcgcggtgc
     5461 caggaccagg ctgagccgaa aaccgcggtc acttcttctt tctgatacct ttctttgctg
     5521 cgggagcctt cttcacgggg gccggccatc cggcaacctt cgccttatcc acatcgaagg
     5581 tcagcatgaa cgggtccttc gtgaattcgc tggccacgcc ctcaaactcg atctcggtgc
     5641 caggctccgc gaccccggcc atcgcctcgt cgagcttcag cgtgacctcg ggggtggacg
     5701 cgtcgctgat cgccagcacc agttccttcg ggttcttggc cggagattga gacacgagct
     5761 tgcccttgaa ctttgtaact cccgaggcgc cacccggtag cccggcgccc ttcacgctgc
     5821 cctcccagta ctgcgggccg ttcggaccaa tgagttccga acggacgttc agccagagcg
     5881 ccagcatcgg gttcgaccgc ttgagttcct cttccttctc cagcgcaacc tcgtaagaac
     5941 tcttgatctt gaagccctcc ggcggcgtgg cgctcgtctt ggcagcctga cgaagtgcat
     6001 cgatgccctc ggccgaaccg tggaaggaat tgtagacttt ctcgaagtac ttgttgatct
     6061 cggtgcggcc ttgagcgctc aatgcaccgg gaccctcata cgtggaggcg cgggcgaagt
     6121 gaaagagagc ctgggcctgt ttctccgcca ctctctggga caccagcacc gtgccgagcc
     6181 agtaggagac ctccccggca ttcgggttgt ctttcagact ttctttgaag tgtttctcgg
     6241 cggcggggta atccttctgt tgcatcg
//
LOCUS       DATIFQ010000447        36413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_916834, whole genome shotgun sequence.
ACCESSION   DATIFQ010000447 DATIFQ010000000
VERSION     DATIFQ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 36413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36413
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916834"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VLE22_22235"
     CDS             complement(<1..1100)
                     /locus_tag="VLE22_22235"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17186.1"
                     /translation="MQDSNRRDFLKTAGAVLSTTVLSSNVRGANDRIAAAFIGMGRMG
                     IGNLDVAMKQPGLEPVAVCDCYQPNLEAAVARSSGRAKAVKDFRDVLADKSIDVVCIS
                     TPDHWHAYMTVEACKAGKDIYVEKPVCCTVDEGLKMVQAARKYNRVVQSGTMQRSGVH
                     FQRACEIVRTGQLGKVTFVRTWNYGLAKPEGIGNPPDTDPPEGLDWDMWLGPAPKHAF
                     NKNRFGVDPNAFSHFRWFWDYAGGMMTDWGIHWLDIVQMAFKEEMPKAIVALGGKYYL
                     QDNRDTPDTLQVTYEYPSGVIATYENRNGNGQSMFNKGGGILFHGTKGTLFVDRTEYR
                     VVPEPGSELAAVNEKASNNTNMAHWANFLECV"
     gene            complement(1106..3568)
                     /gene="leuS"
                     /locus_tag="VLE22_22240"
     CDS             complement(1106..3568)
                     /gene="leuS"
                     /locus_tag="VLE22_22240"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HSB17187.1"
                     /translation="MPEKPYDHNQIELKWCERWQDANLFEAKEDPSRLKYYLLEMLPY
                     PSGTLHVGHIRNYTIGDALARYKWMRGFNVLHPMGWDSFGLPAENAAIANQRHPREWT
                     LENIAYMKKQHKRYGFSYDWDREVSTCEPEYYRWNQWFFLKMLKRGLAYRSNALVNWC
                     PECATVLANEQVVDGCCWRHESTVVEQRSLEQWFLKITAYADELLRDIEKLEGGWPER
                     VLIMQRNWIGRSEGTEADFRLEETGEPIRVFTTRVDTIFGATCVILAPEHPLAARLVR
                     SEDQARLKAMVDDRARRDPGDLVKDGFFTGRHAVNPYNGEKVPIWIGNFVLMGYGTGA
                     IMAVPAHDERDFEFCTRFGIPVRPVIRPVDGELADTATMKGAFVDDGVCENSGEFSGL
                     PSAEARRRMNEKAEREGFGKAAITYRLRDWGISRQRYWGTPIPVVHCPHCGVVPVPES
                     DLPVVLPLDVAITGKGRSPLENVPEFVNVACPKCGATARRETDTMDTFIDSSWYFYRY
                     CDAHNDKVPFDSKKIAYWFPIDQYIGGVEHAILHLIYSRFFTKVMRDLGMIANDEPAK
                     RLFTQGMVIKDGAKMSKSKGNVVSADAAAERYGSDTARLFVLFAAPPEKDVDWQDGGV
                     EGMYRFLGRVYRFVTRNADRIRSAGATGDPSAEADRRILRKLHQTIRKVTEDFESRWH
                     FNTSIAAVMEFVNELTPAETQISPAAMREALEKLILLLAPFAPYLAEEMWEEIGRTGP
                     LCRQAWPDFDPELAKEDLAEVVVQVNGKLRSRLWVPFGTPQDDLKAKALADERVRPFT
                     DGKQVMKVIVVPDKLVNIVVKG"
     gene            complement(3529..4413)
                     /locus_tag="VLE22_22245"
     CDS             complement(3529..4413)
                     /locus_tag="VLE22_22245"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HSB17188.1"
                     /translation="MVRLRIGIHTSIAGSLDQAAVRAAELDANTFQIFSSSPRMWRAR
                     TPSSDEIRNLRETCERHDLTPLVIHDNYLINLAAADPKIRAQSIAAFRGEIERALALG
                     AEHLVAHPGSYKNQTLEQGMRTLVESLVKAAKGLRGPLTLLLENTAGSGSAIGSRFEE
                     LAELRRLASDRVGFEIGFCLDTAHCLASGYNVATRAGLEETVREADRILGLAKVHVIH
                     ANDSKTPLGSRVDRHEHIGEGYIGIPGFRRILNHPRLRSKAFILETPIDRDGDDRRNL
                     ETLKNLCRKSRTTTTRSS"
     gene            complement(4472..4969)
                     /locus_tag="VLE22_22250"
     CDS             complement(4472..4969)
                     /locus_tag="VLE22_22250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17189.1"
                     /translation="MPRTTPTDFLALLKVLRNHGVDFIVVGGVGAVLHGAPVSTFDLD
                     VVHSRDDANIERLLGALDELESYYRTQPEKRLRPGASHLSSPGHQLLMTRFGPLDLLC
                     AVGRSRDYTALLRHSVEMLIGQGVHVHVLSLETLIAVKEETAGDKDLATLPILHRTLA
                     EKSKP"
     gene            complement(4947..5153)
                     /locus_tag="VLE22_22255"
     CDS             complement(4947..5153)
                     /locus_tag="VLE22_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17190.1"
                     /translation="MPAQTWLCHNPTMELQPETAPGAPSISEDGVDLTLIRWMLSLSP
                     AERLEVLQQFVTSVERIRAENDAD"
     gene            5208..6797
                     /locus_tag="VLE22_22260"
     CDS             5208..6797
                     /locus_tag="VLE22_22260"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSB17191.1"
                     /translation="MKLARLRRWSQLVFLALFLFLLIRTEFRGSFAATKGDIRLSYPV
                     SIFLEADPLAAVTTALSTGALYKGLLWSLVILIPTLFLGRFFCGWVCPLGTLHHFFSS
                     LKSEKKRGKQRLDSNRWKRWQNLKYYILIAVLVAAAFGSGLAGILDPIPLIVRSLALS
                     VLPAVNYALNATFDISVLSFKQPYFRQGFFFGLLLVAIIALNFRITRFWCRALCPLGA
                     LLGLASRWSILGIEKRASQCGDCNRCLLHCQGGDDPIPGATWRKAECHLCFNCVADCP
                     EAGGIRFRFFPTGTATAVAPDLQRRKAVTGLVAGVAALPLFRSTTGLKSEHDSRLVRP
                     PGALDEKSFLERCIRCGQCMKVCPNNALHPTLAQAGVEGLWTPVLIPRVGYCEASCTL
                     CGQVCPTGAIWEVTGAEKGWSVEGNGGDAKPVRIGTAFYDHGRCLPWAMARECIVCEE
                     WCPTSPKAIYLVPAEVKDSEGSPKRVRQPYVNPELCVGCGACEYACPVLDRPAIYVTS
                     IGESRSRNNQFLLKRPKKGKA"
     gene            6794..7282
                     /locus_tag="VLE22_22265"
     CDS             6794..7282
                     /locus_tag="VLE22_22265"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HSB17192.1"
                     /translation="MKGRLAVAAMFVMLTACGPKTETATKSFFPETGEAAGWTKAKET
                     RTFEAKDLWQYIDGDDQKYLKAGVVRTQTTDYRYQDKIDAVADIYIMGAADGARKVYE
                     TESGEGSTPASLGDGGRLYGASAVFHKGRYFVRLTAYESTPEIGKALLELGKLIEKRI
                     GD"
     gene            7298..8500
                     /locus_tag="VLE22_22270"
     CDS             7298..8500
                     /locus_tag="VLE22_22270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17193.1"
                     /translation="MKRREFLKSSAGMVAGIGVTQSCRAVQPRAVEEAAKKVGMLPRR
                     KLGGSGREITVLVGASSWSMDAVEAGIRCGINFWHKAEDWSAADVPQAIRKNREAHYC
                     QVCVDRVRGNHETGAINEESHYQLVRQAVKRTGLGYFDDMQFHFGYHSVTEVKNNHGF
                     VRAYERLKKEGLVKHLCLSQHSYNGNWRVPGGESAWEILTAVVDQRLYEHAQIIYTYG
                     DDAAANEFLAMARRKGFGTIAMKTTRGASRMLQDQAFMKTFPKGTSPHHALARWLTTR
                     TQLDGAVIHISGLDEFADTCAGAGKAMRAADTRAIEQMTVYANAEVCRLCNDCMSHCA
                     HGIAVADILRYERYALDYGDGERARKLYAELDRRGDSCTACGTCLPHCGQGLDIPGKL
                     VRVHSLLG"
     gene            8521..9444
                     /locus_tag="VLE22_22275"
     CDS             8521..9444
                     /locus_tag="VLE22_22275"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB17194.1"
                     /translation="MTRREALTACFGAAAFAPPAKSRVVIAREPKLDAARLPKLLDRA
                     MQAYFSCDSPVETWKRIVRPNEVVGLKVNCLAGKGISTNVPLVDAICERLRQAGVPVG
                     NIVIWDRLNDDLDSAGFRVATRADRIRCAGNDVLGYEGELSVFGSAGSLMAKTLTRVC
                     DVVINLPVLKDHGIVGATLALKNMFGAIHNPNKYHINTGDPYVPDVYMLQPVRRKVRL
                     AICDGITAQYEGGPSYMPQWTWRFHGLLVAEDPVALDYTGWQIIEQKRAEKGMKSLKD
                     AGREPRYIATAADPQHRLGTNDPRQIERVEV"
     gene            9452..10531
                     /gene="amrS"
                     /locus_tag="VLE22_22280"
     CDS             9452..10531
                     /gene="amrS"
                     /locus_tag="VLE22_22280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HSB17195.1"
                     /translation="MDRRSFLMTAALPCLGAAGDAQFTVEAKHYEKQPHKKIKCKLCP
                     RECVIDDRERGYCGVRENRGGVYYTLVHSRVCAAHVDPIEKKPFFHFHPGTLAFSIAT
                     AGCNVNCKMCQNWEISQVRPEQVRNVYLPPRRAAEMAKESRCGAIAYTYSEPVIFFEY
                     LLDVARAGHAAGIPSAVVTGGYIRPEPLKELCGAVDAIKVDLKAFSEKFYKEVVNGEL
                     KPVLEALVLIRKLGMWSEIVYLVIPTLNDGDAEFLGLAKWVKAELGPDVPVHFTRFHP
                     EYLLKNLPPTPVKTLERAKSIADAEGLHYVYVGNVPGHPAENTYCPRCRKAVVERQGY
                     DVGGVHISKGKCQFCQQPVAGVWGV"
     gene            10531..12045
                     /gene="amrB"
                     /locus_tag="VLE22_22285"
     CDS             10531..12045
                     /gene="amrB"
                     /locus_tag="VLE22_22285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HSB17196.1"
                     /translation="MRRHAMWALVLVAAAGALCAQTVRKPAVAGAFYPADAKELSTSV
                     DGYLAKATPPEIKDLVALVAPHAGHMFSGGVAGYSYALLKGRKIDRVVIISPSHIEAF
                     PFSAVFNGTAYETPLGQVPVDREFAAQLAKGNELVKLSSRGHIATSGRGEHALEVQLP
                     FLQRALGKFQLVPVIMGDQSYEACRALGMALAKLVRGPETLIVASSDLSHFHKYDEAV
                     NLDRKTLRAIEEWDYLSMSRNFDLRVWEACGGGPIIAAMIAAQRLGAKQAKVLKYANS
                     GDVTGDRSRVVGYGAVALFKGQTADGRGAAAFSLSDAEEDELMALARKSVETAVKQQK
                     LYECPASAREPLMQERGAFVTVKKNGKLRGCIGYAAPLKPLYLTVRDVAAMAAVRDIR
                     FPPVGTNELPELQYEISVLSPLRRVLDVNRIEVGKHGLVVKKGSKEGLLLPQVPVEQG
                     WDRITFLQQACVKAGLPVGAWKDEDTDVFAFTALVFGEQAGSGTVNGKRSAAGR"
     gene            complement(12014..14296)
                     /locus_tag="VLE22_22290"
     CDS             complement(12014..14296)
                     /locus_tag="VLE22_22290"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSB17197.1"
                     /translation="MNSAVMLPRAILLVTGFSAVIAQIVLMRELMVVFHGNEASLGLM
                     LASWLAWTAFGSAVLGRFSARSARPRRPAAVLASLSAVTLPLAIAAVRYARSFFQTVP
                     GEVLGPGQTLLAAVVVLAPFCAVSGALFAAGSRLWTAEISVAPGEATGTAYLIESVGG
                     AVGGLLASLALIQSLSPFEIAALLAWLNLSVAGLLLAGVGNRRQVAVLVLGASLVPFL
                     FTGARTLERRTLAREWSGFHVAETIQSSYGNLTLVTSEGSATLYQNGLPIFTVPDPAA
                     AEESVHYALLEHSAPRTVLLIGGGVNGSVAEALRHPTVGRLDYVELDPKILDLARRRF
                     PEEYSAIGSDSRVHVHSGDGRFFLKTAAVRFDVIIVNLPDPRTAQLNRFYTLEFFREA
                     AGKLNAGGLLSLRLSGTENYIGPELAGFLRSIHKTLRLVFPEVAALPGGNIHFFAAAK
                     PGTLASGANELIARLRSRNLRTTYVREYYLPFRMMPDRLADLESQIRPLAETPVNRDF
                     TPVAYYFDIALWSARFHPEWAGVFRALARVNFHLLVLVTLLVFAIAAALLRRVAPGDR
                     RRSVAGFCVASMGFTLMALQVLLLLAFQAVYGYVYHQLAVLIACFMGGIALGSWLALR
                     RPLSGMRPLAVLQAVAGVSAPLLVGASILLARPASPTGLFIAGQLAFPALAVASGLLG
                     GFQFPMASRVFFEKDSRFKGLGALYALDLAGACIGALTLSAWLVPVFGFGRTAFLVAA
                     VNLVPAAMAALTFPRRFVSR"
     gene            14338..15324
                     /locus_tag="VLE22_22295"
     CDS             14338..15324
                     /locus_tag="VLE22_22295"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB17198.1"
                     /translation="MESTRREAVGKIAKLAGLSAGTAGLGLWLRGRSRRPEEPVPTEL
                     RKSFGVAPNANLPEMVVARGEEPGALVRRAIEAMGGISRFVSRGDVVLVKPNVGWDRT
                     AEQAANTNPEVMAEVVRLCLGAGAKRVVVTDVSTNEPRRCFQRSGIGRAAKAAGAQVI
                     LPEDRLFRDVNLRGEVLTVWPVLQPFLEADKMINVAIAKHHSLTGATLGIKNWYGILG
                     GPRHQLHQHIHESLADLADFMRPTLTLIDAWRVLLRNGPTGGNLEDVSVEKTLIVGTD
                     PVAVDSWAAKAYWGMDQRSLPYLEIANARGLGQYDLTKVRMKSEGVDNLERD"
     gene            complement(15292..16287)
                     /locus_tag="VLE22_22300"
     CDS             complement(15292..16287)
                     /locus_tag="VLE22_22300"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HSB17199.1"
                     /translation="MSSKALHMRFTLCLAALLCVVPAFAGADYFPLQAGNQWVYRGSG
                     IRAAETMKLEVTQSAEFDGRTYYLLDGLGESDHWLRMGEDGSLLRYDPETKQEEVWYH
                     FGAAEGEKYNTRLPGVCCGLAEITSRKAEYEGPVGWFDYALEVRYPGTFQVGIDREVF
                     LPYVGLVNRGQGTGGPGYAHYDLIYARLGGVTHISEPELGFGLTLDSAVYTINLMPSP
                     DPRQAQRLTARLTLRNTGWEPAMLVFPSGQDFDLLLRNDKGEVVYQWSTGKFFPEAVR
                     RLPFGPGEKNFAFSVPLSDKEGNPLAVGRYVAEAWLATQPRSYAASVTFEVVHAF"
     gene            complement(16277..17230)
                     /locus_tag="VLE22_22305"
     CDS             complement(16277..17230)
                     /locus_tag="VLE22_22305"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HSB17200.1"
                     /translation="MLLGAAAFAAEPDYFPLHPGNQWVYRCTGNCGDELPVLEIARDG
                     SFNGTWYALLRGFHGRETWLRQDSDGVLWALDPDSGLETRWYSFFAPVGEPFDTGADP
                     CSRSASVVTREFDYRGPAGRFDDVLQLRYGERACADAGANEEYFLPWTGIVRRVETTI
                     AGPRTYDLIYSRTGGVTVIAEPHLSFGLHIDRSLYDVDFMPPVDPSRSIPVMTARIAL
                     TNATDQPVSLVWPSGQRYDLEIRNERGAVVWRWSDGKAFPAVFGEEKFGPGERNHTIL
                     VPLAGRDMKTLPPGRYTVTAWLTTEPIVYRASVGFEVRHVF"
     gene            complement(17232..18200)
                     /locus_tag="VLE22_22310"
     CDS             complement(17232..18200)
                     /locus_tag="VLE22_22310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB17201.1"
                     /translation="MRYRKLGRTNLEISEIGYGAWGIGQTQWLGGTDSEAVAALRRAI
                     ELGLNFIDTALAYGDGHSEQLVGGILREAGKTIYVATKVPPKNDLWPAQPGVGINDVF
                     PYEYVMASTEQSLRNLGRDVIDLQQLHVWNPEWTGRDEWRCAFEDLKRSGKVRFVGVS
                     INDHQPESALDLIRTGLIDTVQVIYNVFDQSSERSLFPLCTERNIGVIARVPLDEGSL
                     TGSITPDTVFPKGDFRDWYFRGNRKAEVAERVDALKKDLSWVAEPLPEVALRFCLSHP
                     AVSTVIPGMRKSRNVEANCRVSDRGPLDEPVMAVLRRHAWNKNYYC"
     gene            18274..19053
                     /locus_tag="VLE22_22315"
     CDS             18274..19053
                     /locus_tag="VLE22_22315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17202.1"
                     /translation="MLRRALILMVCVVASWAASLRLYLKDGTYHVVREYEVLEDRVRY
                     YSVERGDWEEIPLDLVDLEKTKSEVRERRESIEKEAKVLSEEEKVEREQREEAARVPQ
                     DVGVFWVTGTQLQALKQAEPKVVTSKRRSILKILSPVPMVAGKATVELDGEQSANVVT
                     TVRPEFYFRLAVEERFGIIKLTPKNGARLVEKWSVAPVTNDIFAEQQHVDILRQQVDD
                     GVYKIWPKQPLEPGEYGVVEYTEGKGNTQIWDFSCRPAVNP"
     gene            19138..22461
                     /locus_tag="VLE22_22320"
     CDS             19138..22461
                     /locus_tag="VLE22_22320"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB17203.1"
                     /translation="MYSLKTLSYRLALFITFTTGLCFPQSDTGTIVGIVRDASGGVVP
                     QAAAAVTNVSTGAVWRGFTNDEGFYRIANLPPSTYEVEIEAKGFRRSIGPPARVSAGE
                     VLRVDVAVEVGPVTEVVNVDAPAIAVNTEDPQLGKVVRDPAGLPVLSGANGRNALWLA
                     ATQPGVVFAGQLASQDGGRFSANGQRAQSNNYMLDGADANDLAANTPDSVQSISPNAL
                     EEFRIITGAMKAEYGRNTGAVVQVVTRSGANTFHGGASEYFRNTKLNAAPFFQKAVPG
                     GTKDRFANGFPRKPQWNSNDFDANLGGPVVREKTFFFASYLGFRRRQGVSRSATVPND
                     EQRALIESQGTAEAKALLALVPRASTGNTLFSSPSNARTRDQGMAKLDHYFSPANRLA
                     VTYFIDSMSLDSAPFAFGGSNVPGFGADTTQRFQHVILRDTHTFSPSLLNEFRASYHR
                     NASVGNVPQNRTTMDSLGLHGIVPDDRDGEGPPWVIITGFSEFGNTIQGPQGRADNTF
                     QYIDNVSWTRGRHYAKFGGEFRTYAQNQIFTFVNNGYIYIDGSGTSLEMVPQIHGLSA
                     PLNDFANGFATAYIQNSAGRPGLRTRSVNLFFQDDWKLRSYFTLNAGLRWEYNSSLKE
                     LQDKVAGFRAGVKSSIYPDAPAGMVFPGDAGISRSTYDEDLNNFGPRVGFAWDVLKNG
                     RLSLRGGYGLFYDVSITELTLPFITTPPYAIQPSVAFVDYADPWASSRVQPIAQPFPF
                     RPVGAGESFNFSSIAPISLTVMDPHFATPYAQHWSLQVQYQAAKDWLVDVGYAGSNGV
                     KLLNRRDINPGVPRPGASAGNLDLRRVYNTSHPEKVEFGGMPFADITNQNTDANSNYS
                     SFQAGVTKRFVGGFAMTHAYTWAHSIDNKSGLRGSLARSDSAAADRGNSEMDVRHRYV
                     ATYEYEFPWMAAQSGMLGRIAGGWGVSGVTTFQTGLAFDITEATDRCLCGSGGQRPDY
                     IAGTVTFYDPRSVSAVPGRANSWFDGTGGGTATAATNPFFRRVGSAATYAAGAGRFGN
                     FGRNVLHGPGINNWDFAAFKRVRIREGHQVNFRAEFFNLFNHAQFEIPNGSIASVNFG
                     RVTTTREPRIVQFALRYSF"
     gene            22551..23270
                     /locus_tag="VLE22_22325"
     CDS             22551..23270
                     /locus_tag="VLE22_22325"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17204.1"
                     /translation="MRDFRIRYRNMSLGVFWSLLNPLVMMGVLTFVFTKIFSSDTPNF
                     HVFVLCGVVPFNFFALAWVSGTMAVFSNSGLVKRVLLPREIVPISTVLANCLHFLIQV
                     ALLLAVTLLAGYRVNVYWLLLPLVFGLEVVFVCGLSLASSALDVYFRDMRYVVESMNT
                     ILFWLVPIFYSFTAIPARFHPVYQYNPIAAVVLACRHILMEGLAPPVSLLYKLAIVST
                     LSLLAGLWIFGRLKRRFADYL"
     gene            23272..24012
                     /locus_tag="VLE22_22330"
     CDS             23272..24012
                     /locus_tag="VLE22_22330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB17205.1"
                     /translation="MTGVVQLDSVSKVYLRRSREFLHAFLSGWLRRAPKGGFYALKNV
                     SLRLDEGDSLAVIGANGAGKSTLLSVIAGLSWPDEGTVTVNGRIAALLELGSGFHPDL
                     TGAENLRLNAAVLGLTRRKTEERFDEIVEFSEIGDFIDEPLRTYSAGMIMRLAFSVAM
                     NVQPDILLIDEVLAVGDQAFQRKCMEGILELKRAGRVLICTSHVAETLQQLCSRAIWL
                     DHGRVIREGPVTEVLDEYQSMRTGAGKR"
     gene            24077..24451
                     /locus_tag="VLE22_22335"
     CDS             24077..24451
                     /locus_tag="VLE22_22335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17206.1"
                     /translation="MLNLPVPEGQEQQAGEDKAAASKLLLAELAVGVRELRRRANRVG
                     LVPEGYPLPLAIIMKTIHRLLPWYTRSIQAHAQQAASSMDTILICLQAIVQSQERIAE
                     RLDRLERETRRPAEAGSPPQQQ"
     gene            24448..26691
                     /locus_tag="VLE22_22340"
     CDS             24448..26691
                     /locus_tag="VLE22_22340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB17207.1"
                     /translation="MSAEGTARSERFGGPPVSVVINTYNRAGHLRRCLDALTKQTYRN
                     FEVVVVQGPCTDRTADVLAHFGDAITIAATETRILGVSRNVGIAHSHGELIAFTDDDA
                     VPDPGWLANLVPAFDDPEVGAAGGMVYRMGDGSIEWRNGILDRDGMLDPIRPEPGPFP
                     RRRDGRLNTVSGNCCMFRREALEENGGFDEAIVFMYDESDMVQRMELSGYSVIHRPDA
                     VVFHESARSEMRENRLDYNWFVICKYHAYGALKNARAPHSWAAVRIAWRLIDRRMLVN
                     LRWWGRRLIGSRQLLRFESQCLRGIAAGLWKAWNRPAAVQTRLPAAETSPIRPFCYTP
                     ARRLRVCLLSQSTPERSPDGIATYTFTLARALFEQGCDVHVISRGEEERSSLSEGIWR
                     HEVTPENISARLGLGSEFPATAENLDYANAAWRKIRELDERNGLDLVEASSWNSEGLI
                     AALDGRIPVVIHVHSSLLHVMETQEWEENADRWLCAELEERQSLLADGVVTSTREVAQ
                     SVFSHYKRRPVLTAVIPLGLEIPALRPAAREDRMKKVLFVGRLEPRKGIGVLLEAIPR
                     VLEAVPEAEFDIVGKDVMGASSPVTRWWQAHRSLRRRVRFHGEAPSRALDTLYETCDL
                     LVAPSRYESFGLIYVEAMARGKAVIGTTAGGIPEVVRDAETGLLVPPDDPEALAAAII
                     RLLRDDEYRRSLGQAGYGRYRAEFSAWKMAGRMLDFYREVIARRAAQTPSDANRSESR
                     CSAASEE"
     gene            complement(26631..27704)
                     /locus_tag="VLE22_22345"
     CDS             complement(26631..27704)
                     /locus_tag="VLE22_22345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB17208.1"
                     /translation="MSHTAVDQFNTAVYEGDAVGNEIVTLRAMLRARRLSSAIYSEVP
                     SRTAGVYVQPWSEYHGCEANILIVHYSVFSNLFDQVFAAPSRKVLLYHNVTPPTFFEG
                     MPGELRDRVLAARDGLPRYRSSVELALADSEYNARDLTDAGFLNVRVFPYVLTHRLHD
                     VPPDESVLRRYRGDGLVNLLVVGRIAPNKCIEDCVLAFHSFCNRIEPRSRLFIAGSAT
                     STEYYLMRLQQLVTRLGLDGSVVFTGRVSQAALNAYYRLADAFLAMSEHEGFCVPLIE
                     AMLFDVPVFAYASCAVPETVRGAGILFSEKNHPEMAEAIGVVVSDPVWKERILRAQRE
                     AAAYYSSDAAEQRLSDLLASLGV"
     gene            complement(27701..28156)
                     /locus_tag="VLE22_22350"
     CDS             complement(27701..28156)
                     /locus_tag="VLE22_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17209.1"
                     /translation="MGTPAPEQLGAELREVARRRLERRPFQGSAIDYGWLAATFRSEP
                     GMDPYGFGQLVAEIEQTGGVYPPEPPTLRGRLGRFLIDLEYRVLWWLVRAISRRDQAW
                     RAAYHMMLALHEKQVELRLEALGRISEIEARISLLEERLSRTKPEDRGA"
     gene            complement(28187..29317)
                     /locus_tag="VLE22_22355"
     CDS             complement(28187..29317)
                     /locus_tag="VLE22_22355"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSB17210.1"
                     /translation="MRVAIDAIPLLLRSAGVKTYLFNWIVSLRRVAGDGRVVTFPSLG
                     PLVSYSHERSVLGRLPTLGRLALLHANNYSPVSWLDWLGPKSDVFHASQQLWNPPRRG
                     RLTGTLYDMTCWLVPETHSLANVSAAHRFAQRVLCRADGLIAISESTRRDAVRILGLP
                     PEKIEVIYPGVADAFFRTTPVSAGLAAAKYSLGRPYALFVGTIEPRKNVDRLLDAWAT
                     LSPGLRSEFDLVLAGPVGWAEPSTMARLQSPSSTVRYLGYVPEEDLPGLTRGASLFVY
                     PSLYEGFGLPVGQAMAAGAPVVTSNVSSMPEVAGEAGLLADPKSTAEIARAIETLLLS
                     ESLRTQLSSHGLRLAQRYRWEHCAAASWRFFERVCGQASASR"
     gene            complement(29327..31162)
                     /locus_tag="VLE22_22360"
     CDS             complement(29327..31162)
                     /locus_tag="VLE22_22360"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB17211.1"
                     /translation="MKPSAGSSLPDRRLLGAGDLIILAIAAVIFLGCIPSPPSLMDDV
                     DAVQAQIARNMLESGDWVTARLNGVVYLEKPPLKYWMIAVSYMVFGVHDWAARLPLAL
                     GTVLLCWVAYRFGVWAFGATAGLYTGLCLSACTGLFLFTRILISDVLVTLAVCVSMWS
                     LLCALDEDEPRPRAWALLLAASIGAGLLLKGLIAIVFPLGAGGLYLLLTRQLFAKRTW
                     QRLRPLSGLAVILLIAAPWHILAILRNPPYFDLTMKSGPGVWHGFFWLYFLNEHVFRF
                     LNMRYPRDYNTVPRHLFWLLHLVWLFPWSAFLPATFRLSYKTADRAGHTRFLALCWIG
                     FILAFFTFSTTQEYYSMPTYPAFALLLGSAMAAGGAWVRWGLRAVAAVTAAAAAVISV
                     VLYLVRGMPTPGDISNALARSENVYEVYTLSLGHMGDLTIRSFAYLRLPLVVAGIALL
                     LGAVCSWRWSGRRAFLGLALMMALFAHAARIAMVTFDPYLSSKPLAEALRRAPQGMLI
                     VDDQYYAFSSVLFYAGRTALLLNGRVTNLEYGSNAPSAPDVFIDDERFRDLWARNERY
                     YLVAGRTALPRIERLAGRDGLHRVAESGGKLLLTNQPMLSSELLQ"
     gene            complement(31131..31763)
                     /locus_tag="VLE22_22365"
     CDS             complement(31131..31763)
                     /locus_tag="VLE22_22365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSB17212.1"
                     /translation="MMMLDPFGGWIYRGPREKRSIALTFDDGPSGSTSRLLEVLAHHD
                     AHATFFQCGMHTRRHKDIARATAEAGHEIGNHTDSHRLLALRSRRFLRDELRRAQESI
                     AEATGQTPVLFRPPYGVRWFGLRGVLQELGLLGVMWTKIGRDWKYPADQVLRCLRRGV
                     ANGAILCLHDGRDVEAEPDIENTIEAVRRLLPELKDQGFRFETVSRLIAS"
     gene            complement(31769..32896)
                     /gene="hpnI"
                     /locus_tag="VLE22_22370"
     CDS             complement(31769..32896)
                     /gene="hpnI"
                     /locus_tag="VLE22_22370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HSB17213.1"
                     /translation="MLVAAALALVLAGSLVYCVLTVIAARRYLAVRPPAATRMPPISV
                     LKPLSGADLGLEENLRTFFLQDYPDYELLFAVREADDPAAAIVERLRREFPSVAARLI
                     VTGEPPYANAKVWSLSMMWAAARHDLVVMSDSDIRVTARLLRTIAAEFDDPAVGLATC
                     PYRAVPGQSFWSALEAVGMNTEFLGGVLTARMMEGMKFALGPTIAVRRQALDGIGGWN
                     SLMDYLAEDFVMGNRVAASGYVVLLSSYVIEHHIGATGFVENWRHRLRWARSTRRSRP
                     KGYLGQVFTNPLPLALLICTLRPDLWLLLPVTAVFRVAAAFATAGWVLRDRLTLRMWW
                     SVPLQDIYSFVVWLGGFVGNTILWRGRRYYLRPDGRFELVR"
     gene            complement(32900..34150)
                     /locus_tag="VLE22_22375"
     CDS             complement(32900..34150)
                     /locus_tag="VLE22_22375"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSB17214.1"
                     /translation="MPDERRRGRFEFFVASRYLRAKRKQVVISVITLISVIGVAAGVM
                     ALVVALAINNGFRSTLQRSLLGATSHVTVLEKQPGEGISEWPALVAKLRRLPHVVSAS
                     PTLYGQVFLSSPLQSEGAVLKGIQTGSGSETVGLLRRLKEGRLEALDDQSGLPGIILG
                     SRLSARTGLPLNSVATVITPQGELTPFGPKPSFFRFRVVGIFETGFFEIDYAWAFTSL
                     KTAQRVFTLRDVVNAVELKIDDVNLASDVARSAERVVGPDLAATSWMEQNRQIFNALR
                     MEKTVTIVTIGLIVLVAALNILIALVMMVMEKYRDIAILASMGARREQIRRIFVWQGL
                     LIGTVGTVIGLATGYTLCFLADRYRWLRLDEEVYALSYVPFDPRWIDAVWIAAAAILI
                     SYLATIYPSRNATRITPAEALRYE"
     gene            complement(34143..35645)
                     /gene="lysS"
                     /locus_tag="VLE22_22380"
     CDS             complement(34143..35645)
                     /gene="lysS"
                     /locus_tag="VLE22_22380"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HSB17215.1"
                     /translation="MSLEQELLEQRLKRIREIEALGFRPYGSRFDFTHTIPEVLAIWG
                     PKTEEQLSDKMNVRVCGRVQTVRRMGKAGFVHLMQNGERIQIYIRKDAVSERDFSLYQ
                     NLDIGDIIGVEGYIFRTRTGELSIHAERLVHLAKTLLSMPEKWHGLEDVEVRYRQRYL
                     DLIANPEVRKAFVTRARIVSSFRRRLEERGFIEVETPMMQPLYGGATARPFVTHHNTL
                     DIDLYLRIAPELYLKRLVVGGLERVYEINRNFRNEGISTQHNPEFTMLEFYQAYTDYR
                     GLMDFSEELLRQVTVDATGSVEVDYQGTKIDFSRLRRFSMREAIIEFWKDGERPSMDD
                     LRDPEWLRKRSGKPSAGEALVDLFERIAESHLIQPTIIYDYPVETSPLSKNKPDEPGM
                     VERFEIYAAGMELGNAYTELNDPLEQRRRFEMQVKMRESGDEEAHQMDEDFVRALCYG
                     MPPTGGEGIGIDRIAMILTNSKSIRDVILFPLLRPEGPIGLSDRLGDLDA"
     gene            35686..>36413
                     /locus_tag="VLE22_22385"
     CDS             35686..>36413
                     /locus_tag="VLE22_22385"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HSB17216.1"
                     /translation="MKSISIVIPAYNEENRLPATLDRVLEYLDSRHFPPSEVIVVDDG
                     STDGTAVAAVRYGESDSRVRLLRNPGNRGKGYSVRHGMLEAEGEWRLFTDADLSAPIE
                     ELDKLLAAARDSRASVVIGSRALNRSLIQVHQSFFRETAGRVFNLLMRLLTGLPFWDT
                     QCGFKLFDAHAAREVFRRQRIEGFGFDVEVLFIARKLRFHAVEVPVRWSHVEGTKVRM
                     LKDSMGMFADLACVRWNQLRGLYR"
BASE COUNT         7077 a        10707 c        12167 g         6462 t
ORIGIN      
        1 ttcacgcact ccaggaagtt ggcccagtga gccatgttgg tgttgttgga ggccttctcg
       61 ttcaccgccg ccagctcgga gcccggctca ggcacgaccc ggtattcggt acggtccaca
      121 aacagggttc ccttggtgcc gtggaagagg atgccgccgc ccttgttgaa catcgactgc
      181 ccgttgccgt tccggttttc gtacgtggcg atgacgccgc tcgggtactc gtaggtcacc
      241 tgcaaggtat ccggagtgtc gcggttgtcc tgcaaatagt acttgccgcc gagagcaacg
      301 atggctttcg gcatctcttc cttgaatgcc atctgcacga tgtcgagcca gtggataccc
      361 cagtcggtca tcatgccgcc ggcgtagtcc cagaaccagc ggaagtgcga gaaagcgttt
      421 gggtcgacgc cgaaccggtt cttgttgaag gcgtgtttcg gcgctggccc gagccacatg
      481 tcccaatcga ggccctcggg cggatccgtg tccggcgggt tgccgatgcc ttccggcttc
      541 gccagtccgt agttccaggt gcgaacgaag gtgactttgc cgagctggcc ggtccggacg
      601 atctcgcaag cgcgctggaa gtggacgccg gaacgctgca tcgtgccgct ctgtacgacg
      661 cggttgtatt tccgcgccgc ttggaccatc ttcagccctt catccacagt gcagcagaca
      721 ggcttctcga cgtagatgtc cttgccggcc ttgcatgctt cgacggtcat gtaggcgtgc
      781 caatggtccg gcgtggagat gcagaccacg tcgatcgact tgtcggcaag gacgtcgcgg
      841 aaatccttga cggctttcgc gcggccactg ctcctggcga cggcagcttc caggttcggc
      901 tggtaacagt cgcagaccgc gaccggttcg aggccgggct gtttcatggc aacgtcaagg
      961 ttaccgatgc ccatgcggcc catgccgatg aacgcggcgg caattcgatc gttggcgccg
     1021 cgcacattgg aactgagaac agtagtgctg aggaccgctc cggcggtctt tagaaaatcc
     1081 ctgcggttgg agtcttgcat gaaacctatc ctttgacaac gatattcacg agcttgtccg
     1141 gcacgacgat aaccttcatc acttgctttc cgtcggtgaa gggccggacc ctttcatccg
     1201 ccagcgcctt cgccttaaga tcgtcctgcg gagtaccgaa cggtacccaa agacggctcc
     1261 gcagcttgcc attgacctga acgacgacct ccgccaagtc ttccttggcg agttccggat
     1321 cgaagtccgg ccacgcctga cggcagagag ggccggtgcg gccgatctcc tcccacattt
     1381 cctcggccag gtacggggcg aacggggcga gcaggaggat cagcttctcg agcgcctcgc
     1441 gcatcgcggc gggcgagatc tgcgtttcgg ccggagtaag ttcgttgacg aactccatga
     1501 cggccgcgat ggaggtgttg aagtgccagc ggctctcgaa gtcctcggtg acttttcgga
     1561 tcgtctgatg cagcttgcgg agaatgcggc ggtccgcctc cgcggacggg tcgccggttg
     1621 cgccggcaga cctgatcctg tcggcgttgc gggtgacgaa gcggtagacg cggccaagga
     1681 agcggtacat gccctcgacg ccgccgtcct gccagtccac gtccttttca ggcggcgcgg
     1741 cgaataggac gaacaggcgt gcggtgtcgg aaccatagcg ctcggcggcg gcgtcggcgc
     1801 tgacgacgtt gcctttggac ttcgacatct tcgcgccgtc cttgattacc attccctggg
     1861 taaacaggcg cttggccggt tcgtcgttcg caatcatgcc gaggtcgcgc atcaccttcg
     1921 tgaagaacct ggagtaaatc aggtggagga ttgcgtgctc gacgccgccg atgtactggt
     1981 cgatcgggaa ccagtaagcg atcttctttg aatcgaacgg aaccttgtcg ttatgcgcgt
     2041 cgcagtagcg gtagaagtac cacgacgaat cgatgaacgt gtccatcgtg tccgtttccc
     2101 gccgggcggt ggcgccgcac ttcgggcagg ccacgttgac gaactcaggg acgttttcga
     2161 gcggcgagcg gccctttccg gtgatcgcga cgtcgagcgg caacaccaca ggcaggtcgc
     2221 tttccggtac cggcaccacg ccgcaatgcg ggcagtgaac gaccgggatt ggcgtgcccc
     2281 agtagcgctg tcgcgaaatg ccccagtcgc gaaggcggta ggtaatcgcc gctttgccga
     2341 agccctcgcg ttccgccttc tcgttcatgc ggcggcgggc ctcggcgctc ggcaggccgg
     2401 agaactcgcc agagttttca caaacaccgt cgtcgacgaa cgcgcctttc atcgtggctg
     2461 tgtcggccag ttcgccgtcc accgggcgga tcaccggacg gacagggatg ccgaagcggg
     2521 tgcagaactc gaagtcgcgc tcgtcgtgtg cgggaacggc catgatggcg cccgttccgt
     2581 aacccatcag gacgaagttg ccgatccaga tcggtacctt ctccccgttg tagggattga
     2641 ctgcgtgccg gccggtgaag aagccgtcct tgaccagatc gcccggatcg cggcgggcgc
     2701 ggtcgtcgac catcgccttg agtcgcgcct ggtcttcgga cctgacaagg cgggcggcga
     2761 gcggatgctc cggggcgaga atcacgcagg tggcgccgaa gatggtgtct acgcgggtgg
     2821 tgaaaacgcg aatcggctcg ccggtttctt ccagacgaaa atcggcctcg gtaccctcgg
     2881 aacgcccgat ccagttgcgc tgcatgatga gcacgcgctc gggccagccg ccctcaagct
     2941 tctcaatgtc gcgtagcagt tcgtcggcgt aggcggtgat cttgaggaac cactgctcga
     3001 gcgagcgctg ctcgactacg gtggactcgt gccgccagca gcaaccgtcc acaacctgtt
     3061 cgttggcgag cacggtggcg cactcggggc accagttgac gagcgcgttg gagcggtagg
     3121 ccagcccgcg cttgagcatc ttgagaaaga accactggtt ccagcggtag tattcgggct
     3181 cgcacgtgga cacttcgcgg tcccagtcgt aactgaagcc gtaccgcttg tgctgcttct
     3241 tcatatacgc gatgttttcg agggtccact cgcgcgggtg gcgctggttg gcgatggcgg
     3301 cgttttcggc cggcaggccg aaggagtccc atcccatcgg gtgcagcacg ttgaagccgc
     3361 gcatccactt gtagcgggcg agggcgtcgc cgatggtgta gttgcggatg tggcccacgt
     3421 ggagcgtccc gctcgggtag ggaagcattt cgagcaggta atacttgagc cgcgaggggt
     3481 cctctttcgc ttcaaagagg ttggcgtcct gccagcgctc gcaccatttc agctcgatct
     3541 ggttgtggtc gtacggcttt tccggcatag gttcttcagt gtttcgaggt ttcggcgatc
     3601 gtcgccgtcc cggtcgatgg gagtctcgag gatgaaggct ttgctcctga gccgggggtg
     3661 attcaggatg cggcgaaacc ctggaattcc aatatagccc tctccgatgt gctcgtgccg
     3721 gtccactcgg gagccgaggg gagttttcga gtcattggcg tggatgacgt gcactttcgc
     3781 gagcccgagg atacggtccg cttcgcgcac ggtttcttcc agcccggcgc gcgtggcgac
     3841 gttgtatccg gaggccaggc agtgggcggt atcgaggcag aagccgatct cgaaaccgac
     3901 gcggtccgat gcgagacggc ggagctcggc cagttcctcg aagcggctgc cgatggcgga
     3961 accggagccg gcggtgtttt cgaggagaag ggtaagcggc ccacgcaggc ccttggctgc
     4021 cttcaccagc gactccacga gcgtgcgcat gccctgctcg agcgtttggt tcttatagct
     4081 gcccgggtga gcgaccaggt gctcggcgcc aagggccagg gcgcgctcga tctctccgcg
     4141 aaacgcggcg atggattgcg ctcggatttt cggatcggcc gcggcgaggt tgatcaggta
     4201 gttgtcgtgg atgacgagcg gagtaaggtc atgccgctcg caggtttcgc gtaagttgcg
     4261 gatctcatcc gaagacggtg tccgggcgcg ccacatgcga gggcttgagg agaagatctg
     4321 gaaggtattg gcgtcgagct cggccgcgcg cacggcggcc tgatcgagcg acccggcgat
     4381 ggacgtgtga atcccgatcc gcagacggac catcgtaaca aagcggcggc cggcgccacc
     4441 gcacgtagaa tgtggccgcg gtccgcgatg actacggctt agacttctcg gccagggtgc
     4501 ggtgaaggat ggggagagtg gcgaggtctt tgtccccggc ggtttcttcc ttgacggcga
     4561 tgagggtttc gaggctgagg acgtgcacgt gcacgccttg tccgatgagc atctccaccg
     4621 agtggcgcag cagggccgtg taatcgcgcg agcggccgac ggcgcacagg agatcgagcg
     4681 ggccgaaccg cgtcatcaga agctgatggc ccggcgagga gaggtgcgag gctcccggcc
     4741 tcaaccgctt ctccggctgc gtccggtagt aggattcaag ctcatccagc gcgccgagaa
     4801 gccgctcgat gttcgcgtcg tccctcgaat ggacgacgtc cagatcgaat gtgcttaccg
     4861 gcgctccgtg caggaccgct cccactccac cgacgacgat gaagtcgacg ccgtggttcc
     4921 gcagcacctt gaggagagcg aggaagtcag tcggcgtcgt tctcggcacg aatcctctcg
     4981 acggatgtga cgaactgctg gagaacttcg aggcgctcag cggggctcaa ggagagcatc
     5041 cagcgaatca gcgtgaggtc cacgccgtcc tcggaaatgg acggcgcgcc gggggcggtc
     5101 tccggctgaa gctccatggt tggattgtgg cacagccagg tctgtgccgg catcggttgt
     5161 tgtgacggtt ccgtgcgtgg cacactgctt gcaggagcga aggtgcattg aagctcgcaa
     5221 ggctccgcag gtggtcgcaa ctcgttttcc ttgccctatt cttatttctt ctaatcagaa
     5281 ccgagtttcg cggttctttt gctgcaacga aaggagatat tcggctctcg taccccgtta
     5341 gcatcttcct ggaagcggat ccgctggccg cggtcaccac ggcgctgtcc accggggcac
     5401 tgtacaaggg cctgctgtgg agcctggtca tcctgatccc gacgcttttc ctcgggcgtt
     5461 tcttctgcgg gtgggtctgc ccgctgggga ctttgcacca ctttttcagc agcctgaagt
     5521 ccgaaaagaa acgaggtaag cagaggctcg actcaaaccg ctggaaacgg tggcagaacc
     5581 tgaagtacta catcctgatc gcagtgctcg tggcggcggc cttcggaagc gggctggcgg
     5641 gaatcctgga cccgatcccg cttatagtgc gatcgctggc tctgtcggtg ctgcccgcgg
     5701 tgaactacgc gctgaacgcg acattcgaca tcagcgtgtt gagcttcaag cagccctatt
     5761 tccgccaggg gtttttcttt gggctgctgc tagtggcaat cattgcgctg aacttccgga
     5821 tcacgcggtt ctggtgccgg gctttgtgtc cgctcggggc gctgctcgga ctggcgtcgc
     5881 gatggtcgat cttgggtatc gaaaagcggg ccagccagtg cggggactgc aaccgctgcc
     5941 tgctccactg ccagggcggc gacgacccga tcccgggggc tacgtggcgc aaagcggagt
     6001 gccacctgtg cttcaactgc gtggccgatt gtccggaggc gggcggcatc cgatttcggt
     6061 tcttcccgac ggggacggca accgcagtcg cgcccgatct ccagcggcga aaggctgtga
     6121 cgggactggt ggcgggcgtg gccgcgctgc cgctgtttcg cagcacgacc gggctgaagt
     6181 cggagcacga cagccggctc gtgcggccgc caggtgcgct cgacgagaag agttttctgg
     6241 agcgctgcat ccgctgcggt caatgcatga aggtgtgtcc gaacaacgcg ctgcacccga
     6301 cgctcgcgca ggcgggagtc gagggattgt ggacaccggt gctcatcccc cgtgtgggtt
     6361 actgtgaggc gagctgcacg ctgtgcggcc aggtgtgccc gacgggggcc atctgggagg
     6421 tcactggcgc cgagaagggc tggagcgtcg aagggaacgg cggagacgcg aagcccgtcc
     6481 gcatcggtac ggcattttac gaccacggac gctgcctgcc gtgggcgatg gctcgggagt
     6541 gcatcgtctg tgaggaatgg tgcccgactt cgccgaaagc gatctacctc gtgccggcgg
     6601 aggtgaagga ttccgagggc agccccaaac gggtgcggca gccgtacgtg aacccggaac
     6661 tgtgcgtagg gtgcggtgcg tgcgagtatg cgtgcccggt gctggaccgg cctgcgatct
     6721 acgtgacgag catcggtgag agccggtcgc ggaacaacca gttcctgtta aagcggccga
     6781 agaaagggaa ggcatgaagg gtagattggc ggtcgccgct atgttcgtga tgttaacggc
     6841 gtgcgggccg aagaccgaga cggctacgaa atcgtttttt ccggagaccg gcgaggcggc
     6901 cggctggacc aaagcgaagg agacgcggac cttcgaggct aaagacctct ggcagtacat
     6961 cgacggggac gatcagaagt acctgaaagc cggcgtcgtg aggacgcaga cgaccgacta
     7021 tcgctatcag gacaagatcg acgcggtggc ggacatctac atcatgggcg cggccgatgg
     7081 cgcgcggaag gtgtacgaga cggagtccgg ggaaggaagc acgccggcgt cccttggcga
     7141 cggtggacgg ctctacgggg caagcgcggt tttccacaag ggccggtatt tcgtgagact
     7201 cactgcgtat gagagcactc ctgagatcgg caaggcgctg ctcgagttgg ggaagttgat
     7261 cgagaagagg atcggagact gagaagggag acgcgagatg aagaggcgag agttcctgaa
     7321 gtcgtcggcg gggatggtcg cgggcatcgg cgtgacacaa tcttgccggg cagtgcagcc
     7381 ccgggcagta gaggaggcag cgaaaaaggt cgggatgttg ccccggcgga aactcggcgg
     7441 gagcggcagg gaaatcacgg tcctggtggg cgcgtcgtcc tggtcgatgg acgcggtgga
     7501 ggccggcatc cgctgtggaa tcaacttctg gcacaaggca gaggactgga gcgccgcgga
     7561 cgtgccccaa gcgatccgca agaaccgtga ggcccactat tgtcaggtct gtgtggaccg
     7621 agtgcgcgga aaccacgaga cgggggcaat caacgaggag tcgcactatc agctcgtcag
     7681 gcaggcggtg aaacggaccg gcctcggcta cttcgacgac atgcagtttc acttcggcta
     7741 ccacagcgtg acggaggtga agaacaatca cggcttcgtg cgcgcgtacg agcggctgaa
     7801 gaaggaaggg ctggtgaagc atctgtgcct gtcccagcac agctacaacg gcaactggcg
     7861 cgtgcccgga ggcgagagtg cgtgggagat cctgacggcg gtggtggatc agaggcttta
     7921 cgaacacgca cagatcattt acacctacgg cgacgacgcg gccgccaacg aattcttggc
     7981 catggcgcgg cggaaaggtt tcggaacgat cgccatgaag acgacgcgcg gagcgagccg
     8041 gatgctccag gaccaagcct tcatgaagac ctttccgaag ggcacgtcgc cgcaccacgc
     8101 gctggcgcga tggctgacga cgcgcacgca gctcgacgga gcggtgatcc acatcagcgg
     8161 gctcgacgag ttcgcggaca catgcgccgg ggcggggaag gcaatgcgcg cggcggacac
     8221 gcgggcgatc gagcagatga cggtgtacgc caacgcggag gtctgccggc tctgcaacga
     8281 ttgtatgagc cactgcgcgc acggtatcgc cgtagcggac atcctgcggt acgagaggta
     8341 cgcgctcgac tacggcgacg gcgagcgggc gaggaagctc tacgcggagc ttgaccgtcg
     8401 gggtgactcc tgcacggcgt gcggaacctg cctgccgcat tgcgggcagg gactggatat
     8461 ccccgggaag ctggtgcgcg tacactcgct tctggggtga gagggcaaag gagccggggc
     8521 gtgacgagac gggaagcctt gacagcgtgc ttcggtgcgg ctgccttcgc gccgccggcg
     8581 aaatcgcggg tggtgatcgc gcgcgagccg aagctggacg ccgcccggct gccgaagttg
     8641 ctcgaccgcg cgatgcaagc gtatttctcg tgcgattcgc ctgtagaaac ttggaagagg
     8701 atcgtacgac caaacgaagt tgtagggctg aaggtgaact gcctggcggg gaaaggcatc
     8761 tccacaaacg tgccgctagt ggacgcaatc tgcgagcgtc tccggcaagc gggtgtgccg
     8821 gtggggaaca tcgtcatctg ggaccggctc aacgacgatc tggacagcgc gggattccgt
     8881 gtggccacgc gggcggatcg catccgctgt gccggcaatg acgtgctcgg ttacgagggc
     8941 gaactgtctg tattcgggag cgccggcagc ctgatggcga agaccctgac gcgagtatgc
     9001 gacgtggtga tcaacctgcc ggttctcaaa gaccacggca tcgtcggtgc gaccctggca
     9061 ctgaagaaca tgttcggcgc catccacaac ccgaacaagt atcacatcaa taccggcgac
     9121 ccgtacgtgc cggacgtgta catgctgcaa ccggtacggc ggaaggtacg gctggcgatc
     9181 tgcgacggaa tcacggcaca atacgagggc ggcccgtcgt acatgccgca gtggacctgg
     9241 cggtttcacg gcctgttggt ggccgaggac ccggtggcgc ttgactacac cggatggcag
     9301 attatcgagc agaaacgggc cgagaaggga atgaaatcct tgaaggacgc cggccgggag
     9361 ccgcgttaca tcgcgacggc ggcggacccg cagcaccggc tgggcacgaa cgatccacgg
     9421 caaatcgaac gggtggaagt gtgaggcggg gatggatcgg cgaagtttct tgatgacggc
     9481 ggcgcttccg tgccttggcg cggccggcga cgcgcagttt accgtcgagg cgaagcacta
     9541 cgagaagcag ccgcacaaga agatcaagtg caagctttgc ccgcgcgagt gcgtgatcga
     9601 cgaccgtgag cgaggctatt gcggcgtgcg cgagaaccgc ggcggggtct actacacact
     9661 ggtacattcg cgcgtctgcg cggcgcacgt cgatccaatc gagaagaaac cgttcttcca
     9721 cttccacccg ggcacgctgg cgttctcgat cgccacggcg ggctgcaacg tgaactgcaa
     9781 gatgtgccag aactgggaga tctcgcaggt gcggcccgag caggtgcgga acgtctacct
     9841 cccgccgcgg agggcggcgg agatggcgaa ggaatcgcgc tgcggcgcga tcgcgtacac
     9901 ctacagcgag cccgtgattt tcttcgagta cctgctggat gtggcgcggg cgggacacgc
     9961 tgcggggatc ccgagtgcgg tggtgaccgg cgggtacatc cggccggagc cgctgaagga
    10021 actttgcgga gcggtggatg cgatcaaggt tgacctcaag gcgttctccg agaaattcta
    10081 caaagaagtc gtgaacggcg aactgaagcc ggttctcgaa gccctggtat tgatccggaa
    10141 gctggggatg tggtccgaga tcgtttatct tgtaattccg acgctgaacg acggcgacgc
    10201 cgaattcctc ggactggcga agtgggtgaa agccgagctt gggccggacg tgcctgtcca
    10261 tttcacgcgg tttcatcccg agtacctgct gaagaacctg ccgccgacgc cggtgaagac
    10321 gctggagagg gcgaagtcga tcgcggacgc cgaggggctg cactacgttt acgtgggcaa
    10381 cgttccggga catccggccg agaacacgta ctgtccgagg tgtcggaagg cagtcgtgga
    10441 gcggcaggga tacgacgtcg gcggagtgca catcagcaaa gggaagtgcc agttctgcca
    10501 gcagccggtg gcgggagttt ggggggtgtg atgaggagac acgccatgtg ggcgctggtg
    10561 ttggttgccg ccgccggggc gctgtgcgcg cagaccgtgc gcaagcccgc ggtggcgggg
    10621 gcgttttacc ccgcggacgc aaaggaactc agcacatccg tcgacggtta cttggcgaaa
    10681 gcgactccac cggagatcaa ggacctggta gcgctggtgg ccccgcacgc agggcatatg
    10741 ttctcgggcg gcgtggcggg atactcgtac gcgctgctca aggggcgaaa gatcgaccgt
    10801 gtagtgataa tctcgccatc tcatatcgaa gcgttcccgt tctccgcagt gttcaacggc
    10861 acggcatacg aaacgccgct ggggcaagtc cccgtggacc gggagttcgc cgcccagctt
    10921 gccaagggga acgaactggt gaagctgtcg agccgcgggc acatcgcgac aagcggccgt
    10981 ggggagcacg cgctcgaagt gcagcttcca ttcctgcaac gcgcgctggg gaagttccaa
    11041 cttgtgccgg tgatcatggg cgatcagagt tacgaggcct gccgggcgct cgggatggcc
    11101 ctcgcgaagc tggtccgggg tccggagacg ctgatcgtgg caagctccga cctgtcgcac
    11161 ttccacaagt acgacgaggc ggtgaaccta gaccgcaaga cgctgcgggc catcgaggag
    11221 tgggactacc tcagcatgtc gcgcaacttc gacctgcggg tgtgggaggc gtgcgggggc
    11281 gggccgatca tcgcggcaat gatcgccgcc caacggctcg gagcgaagca ggccaaggtc
    11341 ctgaagtacg caaactccgg agacgtcacc ggagatcgta gccgggttgt gggatacggt
    11401 gcggtggccc tcttcaaggg acagacagcg gacggccgcg gcgctgccgc cttctccctg
    11461 agcgacgcgg aggaggatga actgatggct ctggcgcgaa agtctgtcga gactgcggtg
    11521 aagcagcaga agctttacga atgtccggcg tcggcgcgtg agccgctgat gcaggagcgc
    11581 ggggcgttcg tcacggtaaa gaagaacgga aagctgcgcg ggtgcatcgg ctacgctgcg
    11641 ccgctgaagc cgctctacct caccgtgcgg gacgtggctg cgatggccgc ggtccgcgac
    11701 atccgctttc cgcccgtcgg gacgaatgaa cttccggagt tgcagtacga gatctcggtg
    11761 ctttctccgc tgcggcgcgt gctcgacgtc aaccggatcg aggtgggcaa gcacgggctg
    11821 gtagtgaaga aaggctcaaa agaagggctg ctgctgccgc aggtaccggt ggagcagggc
    11881 tgggatcgga tcaccttcct ccagcaggcc tgcgtaaagg cgggacttcc ggtgggcgcc
    11941 tggaaggacg aagacacgga tgtgttcgcg ttcaccgcgc ttgtcttcgg cgaacaggcg
    12001 ggtagcggga cagtcaacgg gaaacgaagc gccgcgggaa ggtaagagcg gccattgcgg
    12061 ccggcacgag attcactgcg gcgacgagga atgctgtcct tccaaagccg aagacgggga
    12121 cgagccacgc gctcagtgtg agcgcgccaa tacaagcgcc ggccagatcg agggcgtaga
    12181 gggcgcccag acccttaaac cgggagtcct tctcgaagaa gactctgctt gccatcggga
    12241 attgaaatcc tcccaacaag ccggatgcaa ccgcgagcgc tggaaacgcc aactggccgg
    12301 caataaacag accggttggt gaagccggtc ttgcgagcaa tatcgacgcg ccgacgagca
    12361 gcggtgcgga gacgccggcc accgcttgaa gcactgccag gggtctcatt cccgagagcg
    12421 ggcgccgcag ggcaagccaa ctgcccagcg caatccctcc catgaagcaa gcaatcagga
    12481 cggcaagttg atggtacaca tagccgtaaa ccgcctgaaa tgcgagcagc agcaggacct
    12541 gaagcgccat cagtgtgaag cccatggaag cgacgcagaa accggcgacc gagcgccggc
    12601 gatcgcccgg tgcaacccgg cgcaacaggg cggccgcgat cgcgaaaaca agcagggtca
    12661 cgagaacgag caggtgaaag ttgacacggg cgagtgctcg gaagacgccg gcccactcgg
    12721 gatgaaatcg cgcactccag agggcgatgt cgaagtagta ggcgacaggt gtgaagtcgc
    12781 gattcacggg cgtttcggcg agcggtcgta tctgcgattc gagatccgcg agacggtccg
    12841 gcatcatgcg gaacggcagg tagtactcgc ggacgtacgt cgtacgcaga ttccgggagc
    12901 gcagccgagc gatcaactca ttcgcgccgg aggcgagcgt gccgggcttt gcggcggcga
    12961 aaaaatggat gttgccgccg ggaagcgccg cgacttcggg gaagaccagc ctcagggtct
    13021 tgtgaatcga gcgaaggaac ccagccagtt cggggccgat gtagttctcc gtgccgctga
    13081 ggcgcagcga cagcagaccg ccggcgttca gcttgccggc ggcttcacga aagaactcga
    13141 gagtgtagaa acggttcaac tgggccgtcc gcgggtcggg gagattgacg atgatgacgt
    13201 cgaatctgac ggcggcggtc ttgagaaaga agcggccgtc gcccgagtgt acatggactc
    13261 tggagtccga gccgatggcg gaatattcct ccggaaagcg gcggcgggcg agatcgagga
    13321 tcttggggtc gagttccacg tagtcgaggc gtccgacggt ggggtgacgc agcgcctcgg
    13381 cgacgctgcc gttcacgcct cccccgatca gaagaacagt gcgcggcgcc gagtgttcca
    13441 gaagcgcgta gtggacggac tcctcggccg cggcgggatc gggcacggtg aatatcggca
    13501 ggccgttctg gtagagtgtt gcgctgccct cagaagtcac gagggtcagg ttgccgtagg
    13561 acgattgaat ggtttccgca acgtgaaagc cgctccactc gcgtgctagc gtccggcgtt
    13621 ccaatgtacg tgcaccagtg aacaaaaacg gaacaagcga cgcgccgagc acgagaacag
    13681 cgacttgccg gcgatttccg acgcctgcca gaagtagtcc ggcgaccgac aggttcagcc
    13741 atgcgagcag cgcagcgatc tcaaatgggg agaggctctg aatcagagct aggctcgcaa
    13801 ggaggccgcc cactgcgcct ccaacgctct cgatcagata agcggtcccg gttgcctctc
    13861 ccggcgcgac ggagatctct gccgtccaaa gacggctgcc ggcggcgaac aaagccccgg
    13921 atactgcaca gaacggagcg agaacgacga cggcggccag cagcgtctgc ccgggcccga
    13981 ggacttcgcc gggcacggtc tgaaagaaac tgcgcgcgta ccggacagca gcgatcgcga
    14041 gtgggagcgt cactgccgag agtgacgcca ggactgcggc agggcggcga ggtctcgcgc
    14101 tgcgtgcgct gaagcgcccg aggaccgcgc ttccgaaggc ggtccacgcg agccaggagg
    14161 caagcatgag gccgagcgaa gcctcgttgc cgtggaagac gaccatgagc tcgcgcatga
    14221 gaacgatctg cgcgatcaca gcggagaagc cggtgacgag caggatcgcg cgcgggagca
    14281 tgacggccga attcaaggta gcacttttcc tgtggcatgc tttgggaagg aggctcgatg
    14341 gagagcaccc gccgggaggc ggtagggaag atcgcgaagc tggccggact gagcgccggg
    14401 accgcgggtc ttgggctgtg gctgcgcggg cgcagccggc gtcctgagga gccggtgccg
    14461 acggaactcc ggaagagctt tggtgtggcg ccgaacgcaa acctgccgga gatggtagta
    14521 gctcgcggcg aggaacccgg ggcattggtc cggcgggcga tcgaggcgat gggcggcatt
    14581 tcgcgtttcg tctcgcgcgg cgacgtcgtc ctggtgaagc ccaacgtggg gtgggaccgc
    14641 acggccgagc aggcggcgaa caccaatccc gaagtcatgg ccgaggtcgt ccggttgtgc
    14701 ctcggtgcgg gcgcgaaacg cgtggtagtg accgacgtgt ccaccaacga gccgcggcgc
    14761 tgcttccagc ggagcggcat cggccgggcg gcgaaagccg ccggggcaca ggtgattctg
    14821 ccggaagacc gcctgttccg cgacgtcaac cttcggggtg aagtgttgac ggtgtggccg
    14881 gtgctgcagc cgttcctcga agccgacaag atgatcaatg tcgcgatcgc caagcatcac
    14941 agcctcacgg gggcgacgct cggcatcaag aactggtacg ggattctcgg cgggccgcgg
    15001 caccagttgc accagcacat ccatgagagc ctggcggacc ttgccgactt catgcgcccg
    15061 acgctgacgc tcatcgacgc ctggcgggta ttgctgcgaa acgggccaac gggcgggaac
    15121 ctggaggacg tttccgtcga gaagacgttg atcgtaggga cggaccccgt ggcggtggac
    15181 tcctgggccg cgaaggccta ctggggaatg gatcagcgaa gccttcccta cctggagatc
    15241 gccaacgccc ggggactggg acagtacgac ctgacgaaag tccggatgaa gtcagaaggc
    15301 gtggacaacc tcgaacgtga ctgaggcggc gtagcttcgc ggttgcgtgg cgagccaggc
    15361 ctccgcgacg tagcggccga ccgcgagcgg gttcccttcc ttgtcagaca gggggacgga
    15421 gaaggcgaag tttttctctc cagggccgaa cggcagtcga cggacggctt cgggaaagaa
    15481 cttaccggtg gaccactggt agacgacctc gcccttgtca ttgcgcagaa gcaagtcaaa
    15541 gtcctgcccg gacggaaaaa cgagcatggc aggctcccat cccgtgtttc gcagggtcag
    15601 acgcgcagtc agtctttgag cttggcgggg atcaggggac ggcatcagat tgatggtata
    15661 cacagcagag tcgagcgtca ggccgaaacc cagctccggc tcactgatgt gtgtcacgcc
    15721 acccaggcgg gcatagatca gatcgtagtg cgcgtaaccc ggaccgccgg ttccctgacc
    15781 gcggttgacc aggcccacat agggcaggaa cacctcgcgg tcgatgccga cttgaaatgt
    15841 gccggggtag cggacttcaa gcgcgtagtc gaaccagccg acgggcccct cgtactcggc
    15901 tttgcggctc gtgatctccg ccaggccgca gcagacgccg ggcagtcttg tgttgtactt
    15961 ttcgccttcg gccgcgccga agtggtacca gacctcttcc tgcttcgtct ccggatcgta
    16021 gcggagcagt gagccgtcct cacccatgcg cagccagtga tccgattcac caagaccgtc
    16081 gagcagataa tacgtgcggc cgtcgaactc ggcggactgc gtgacttcga gcttcatcgt
    16141 ctccgccgcg cggatgcccg atccacggta tacccactga ttgcctgcct ggagcgggaa
    16201 gtaatctgcc ccggcgaatg cggggacgac gcacagcagg gcggcgagac agagagtaaa
    16261 ccgcatatgc agcgccttag aagacatgcc gcacctcgaa accgaccgag gctctgtaaa
    16321 caattggctc tgtcgtaagc caggcggtga ccgtgtatcg cccaggaggc agggtcttca
    16381 tgtcgcggcc ggcgagcggc acgaggatgg tgtggttacg ttccccaggg ccgaatttct
    16441 cttcgccgaa gacggcgggg aatgccttgc cgtcggacca gcgccaaacc accgcgcccc
    16501 gttcgttgcg gatttcgagg tcgtaacgct gaccactggg ccagacaagc gacaccggct
    16561 ggtcggtcgc attcgtgagc gcgatacgcg ccgtcatgac ggggatgctg cgcgacggat
    16621 cgacaggcgg catgaagtcg acgtcgtaga gcgagcggtc gatgtgcagc ccgaaactga
    16681 ggtgcggctc ggcgatcacg gtgacgccgc cagtgcggga gtagatcagg tcgtaggtac
    16741 gggggccggc aatggtcgtt tcgacgcgcc ggacgatgcc tgtccagggg aggaagtact
    16801 cctcgttggc gccggcgtcg gcgcaggccc tttcgccgta gcgaagctgg aggacgtcat
    16861 cgaaccggcc ggccggccca cggtagtcga actcccgagt gacgaccgac gcgctgcggg
    16921 agcaggggtc agcgcccgtg tcgaaaggtt cgcccaccgg cgcgaaaaac gagtaccagc
    16981 gggtctcgag tcccgaatcg ggatctagag cccaaagaac gccgtccgaa tcctggcgga
    17041 gccaggtctc gcgtccgtgg aagcccctca acagggcgta ccaagtcccg ttgaacgacc
    17101 cgtcacgcgc gatttcgagc accggaagct cgtcaccgca gtttccggtg caccggtaga
    17161 cccactggtt gcctggatgc agcgggaagt agtcgggctc ggccgcaaac gccgcggctc
    17221 cgagcagcaa gctagcagta ataattcttg ttccaagcat ggcgtcgaag gaccgccatc
    17281 accggctcat cgagcggccc gcgatcagag acgcggcagt tggcctccac gttacggctt
    17341 tttctcattc cggggatgac ggtcgagacg gccgggtggg acaggcagaa gcgcagcgca
    17401 acttccggaa ggggctcagc cacccaggag agatccttct tcaaggcatc gacacgttcg
    17461 gcgacctcgg ccttgcggtt tccgcggaag taccagtcgc ggaagtcacc cttggggaac
    17521 accgtgtccg gggtgatgct tcccgtgagg ctaccctcat cgagtgggac gcgggcgatg
    17581 acgccgatgt tgcgctccgt gcacagcggg aaaaggctcc gttccgagga ctggtcgaag
    17641 acgttgtaga tcacctgaac ggtgtcgatc aggccggtgc ggatcagatc gagggcagat
    17701 tcgggctggt ggtcgttgat cgagacgccg acaaagcgga ccttgccgga tcttttgaga
    17761 tcttcgaaag cgcaccgcca ttcatcccgt ccggtccact ccgggttcca gacgtgaagt
    17821 tgctggaggt cgatcacgtc ccggccgagg ttgcgcaggc tctgctcagt ggacgccatc
    17881 acgtactcgt acggaaaaac gtcgttaata ccgacacccg gctgcgccgg ccacaggtcg
    17941 ttcttcggcg gaaccttcgt tgcgacgtag atcgtcttgc ccgcttcccg gagaatgccg
    18001 ccaacgagtt gctcgctgtg tccgtccccg taggcgaggg ccgtgtcgat gaagttgagc
    18061 ccgagttcga tggcgcgccg aagtgcggcc accgcttcgc tgtcggttcc gccgagccac
    18121 tgggtctggc cgattcccca cgcgccgtat ccgatttcgc tgatctcaag attggtcctg
    18181 ccgagttttc gatagcgcat gaagacaaca ttatactggc tctcgacagg cgtcctgacg
    18241 cgtgtggcgg ggcggtagaa tcaggttaga ggcatgttgc ggcgagcgct gatcttgatg
    18301 gtgtgtgtgg tcgcgtcatg ggcggcgagc ctgcgcctgt acttgaaaga cggcacatat
    18361 cacgtggttc gagagtacga ggtactggag gaccgggtcc gctattacag tgtggagcgc
    18421 ggcgattggg aggagatccc actcgatctg gtcgacctgg agaaaacgaa atccgaggtc
    18481 cgcgagcggc gggaatcgat tgaaaaggaa gccaaggttc tctccgagga agagaaggtg
    18541 gagcgggagc aacgcgaaga agccgcccgc gtgccccagg acgtgggagt gttctgggtt
    18601 accgggaccc aactccaggc gctgaagcag gctgagccga aagtggtaac gagcaagagg
    18661 cggtcgattc tcaagattct gagcccggtt ccgatggtgg ccgggaaggc gacagtagaa
    18721 ctcgacgggg agcagtccgc gaacgtggtc acaaccgtga ggcccgagtt ctacttccgc
    18781 ctcgcggtgg aggaaagatt cggtattatc aaacttaccc cgaagaacgg ggcgcggctt
    18841 gtcgagaagt ggtcggtcgc tccggtcacg aacgatatct tcgcggagca gcagcacgtc
    18901 gacatccttc gtcagcaagt tgacgacggg gtctacaaga tctggccaaa gcagccgctg
    18961 gaacccgggg agtacggagt ggtggagtac acggaaggca aaggaaacac acaaatttgg
    19021 gattttagct gccgcccggc cgtaaatcca taaaattcat caaaaaaagc cttctcaaat
    19081 cggttttttc gcataacata gtaagaaagt gggtggagga gagaaaagga taagtgcgtg
    19141 tactctctca agaccctgtc gtatcgtctc gctctcttca tcacgttcac cactggtctc
    19201 tgcttcccac aaagcgacac cgggacgatc gtaggcatcg tccgggacgc cagcgggggt
    19261 gtcgtccccc aggctgcagc cgctgtgaca aacgtttcga ccggtgccgt ctggcgagga
    19321 ttcacgaacg acgaaggctt ttatcggatc gcgaaccttc cgccgtccac gtacgaggtt
    19381 gagattgaag ccaaaggctt ccggaggtcg atcggcccgc ctgcgcgcgt gagtgcgggc
    19441 gaggtgctga gggtcgacgt cgcggtggag gtcggccccg ttacggaggt agtcaacgtc
    19501 gacgcgccgg ccatcgcggt gaacacggaa gacccgcagc ttggcaaggt cgttcgcgac
    19561 cccgccggct taccggtgct ttcgggcgcg aacgggcgga atgcgctgtg gctggcggcg
    19621 acgcaacccg gcgttgtgtt cgccggacaa cttgcatctc aggacggagg gcggttctcg
    19681 gccaacggcc agcgggcgca gtccaacaac tacatgctcg atggcgctga cgccaacgac
    19741 ctcgcggcga acacgcccga ttccgtgcaa tcgatctctc cgaacgcgct ggaggaattc
    19801 cggatcatca ctggcgcgat gaaggccgag tatggccgca acacgggtgc ggtggtgcag
    19861 gtggtgaccc gatcgggggc gaatacgttt cacggaggcg cctcggagta ctttcggaac
    19921 acgaaactga acgcggcgcc gttcttccaa aaagcggtgc cgggagggac gaaggaccgg
    19981 ttcgcgaacg gattcccgcg gaagccgcag tggaacagca acgatttcga cgccaatctg
    20041 ggcggtccgg tggttcgtga gaagaccttc ttcttcgcgt cgtatctcgg tttccgaaga
    20101 cgccaggggg tctcgagatc ggccacggtg ccgaacgacg aacaacgggc gttgatcgaa
    20161 tcccagggca cagccgaggc gaaagcactc ctggcgctgg ttccgcgggc ctccacggga
    20221 aatacgctgt ttagttctcc ctcgaacgcg cgcacgcgag accagggcat ggcgaaactc
    20281 gatcactact tttcacctgc caaccggctg gcggtgacgt acttcatcga ttcgatgtcg
    20341 ctcgacagtg cgccattcgc gttcggaggg agtaacgttc ccggcttcgg agccgacacg
    20401 acacagcggt tccagcacgt gatcctgagg gacacgcaca cgttctcgcc atcgctatta
    20461 aacgaattcc gggcgtctta ccaccggaac gcgtcggtcg gaaacgttcc gcagaaccgt
    20521 acgacgatgg acagcctggg actgcacggc atcgtaccgg acgaccggga cggcgagggc
    20581 ccaccatggg taatcatcac gggcttctcg gagttcggca acacgatcca gggaccgcag
    20641 ggacgggccg acaacacctt tcagtacatc gacaacgtaa gctggacgcg tgggcggcac
    20701 tatgcgaagt tcggcggcga gttccgcacg tacgcccaaa accagatctt tacgtttgtc
    20761 aataacgggt acatttatat cgatggcagc ggaacgtcgc tcgagatggt ccctcagatt
    20821 cacggacttt ccgcgccgct caacgatttc gccaacgggt tcgcgacggc gtacattcag
    20881 aattcggctg gacggcccgg cctccggaca cgttcggtca acctcttctt tcaggacgac
    20941 tggaaactga ggtcgtattt cacactgaac gcgggcctgc gatgggaata caactctagc
    21001 ctcaaggagc ttcaggacaa agtggcaggc ttccgggcag gcgtgaagtc gtcgatttac
    21061 ccggacgcgc cggcggggat ggtgtttccc ggcgacgcgg ggatcagccg gtccacttac
    21121 gacgaggacc tgaacaattt cggcccgcgc gtgggcttcg cgtgggacgt gctgaagaac
    21181 ggacggctta gtctgcgagg cggatacggt ttgttctatg acgtctcgat cacagaactg
    21241 acgctgccgt ttatcaccac gccgccatac gcaattcaac cgtcagtggc gtttgtggat
    21301 tacgccgacc cgtgggcgtc gtcgcgtgtg cagccaatcg cgcagccatt tccattccgg
    21361 ccagttggcg cgggggagag tttcaacttt tcatccatcg cgccgatcag cctgacggtg
    21421 atggatccgc actttgccac tccatacgca cagcactgga gcttgcaggt gcaataccag
    21481 gcggcgaagg actggcttgt ggacgtgggc tatgcgggca gcaacggtgt gaagttgctg
    21541 aaccgccgcg acatcaatcc tggagttcct cgccccgggg cgagcgcggg gaacttggat
    21601 ctccggaggg tctacaacac cagccatccg gagaaagtgg agttcggcgg catgccgttc
    21661 gccgacatca ccaaccagaa cacggacgcg aactcgaact acagcagctt ccaggctggc
    21721 gtcaccaagc gcttcgtcgg cgggtttgcg atgacacacg cttacacgtg ggcgcactcg
    21781 atcgacaaca agtccggctt acgagggtca ctggcgcggt cggatagcgc ggcggccgat
    21841 cgtggcaact ccgagatgga cgtgcggcac cgctacgtgg cgacgtatga atacgagttc
    21901 ccttggatgg ctgcacaatc cggaatgctg ggccggatcg ccggaggatg gggcgtctcg
    21961 ggcgtgacga cgttccaaac tggcctcgcg ttcgatatta ccgaggcgac ggatcggtgc
    22021 ctgtgcggca gcgggggaca gcggccggat tacatcgccg gcacggtaac gttttacgat
    22081 ccgcgttccg tatcggccgt ccctgggcgc gccaattcgt ggtttgatgg gacgggcggc
    22141 ggcacggcca cagcggcgac aaatccgttt ttccggcgag ttggctcggc ggcgacgtac
    22201 gccgccggcg caggacgttt cggaaatttc ggacgtaacg tgctccacgg tcccggaatc
    22261 aacaactggg acttcgcggc attcaaacga gtccgcatcc gcgaggggca ccaggttaat
    22321 ttccgcgccg aattcttcaa ccttttcaat cacgcccagt ttgaaatccc gaacgggagc
    22381 atcgcttcgg tgaacttcgg ccgggtcacc acaacgcggg agccgagaat tgtccagttc
    22441 gcgctgcgat actccttctg acgcggggcc aggctgtggc atgatggtgt tcctgtacct
    22501 cgcgcgatat gcaaggcggt aacttcggat ttctactcag caatctggtg atgcgggact
    22561 tccgtattcg ttatcggaac atgtctctgg gcgtgttctg gtcgctgctg aacccgctgg
    22621 tgatgatggg ggtgttgacc ttcgtcttca cgaagatctt cagcagcgat acgccgaact
    22681 ttcatgtttt cgtgctctgc ggggtggttc cgttcaattt tttcgcactt gcctgggtca
    22741 gcgggacgat ggcggtgttc agcaactcag ggctggtgaa gcgggtgttg ctgccgcggg
    22801 aaatcgtgcc gatatcaacg gtgctagcga actgtttgca tttcctgatc caggttgcgt
    22861 tgctgctcgc ggtcacgctg ctggcgggct accgcgtgaa tgtgtactgg ctgctgctgc
    22921 ctctggtgtt cgggttggaa gttgtcttcg tgtgcgggtt gtcgctggcg tcatcggcgc
    22981 tggacgtcta tttccgcgac atgcggtacg tcgtggagtc gatgaacacc attctgttct
    23041 ggctcgtgcc gatcttctac tcgtttacgg ccattccggc tcgtttccat ccggtatacc
    23101 agtacaatcc gattgcggca gttgtgctcg cctgccgcca catcctgatg gagggcctcg
    23161 ctccgccggt gtccctgtta tacaaactgg ccatcgtttc aaccttgtca ttgctggccg
    23221 ggctgtggat cttcgggcgg ctaaagcggc ggttcgcgga ctatctctga catgacgggc
    23281 gtcgttcaac tggacagcgt ttcgaaggtg tacttacggc ggtcgaggga gttcctccat
    23341 gccttcctgt caggatggct tcggcgggcg ccaaaaggcg gcttctacgc cctgaagaac
    23401 gtgtcactcc gcctcgacga aggtgacagc ctggcggtga tcggcgccaa cggagcgggt
    23461 aagagcacgc tgctgagcgt gatcgcagga ttgtcgtggc cggacgaggg gacggtcacg
    23521 gtcaacggcc ggatcgcggc gctgctggaa ctcggttcgg gctttcatcc ggacctgacc
    23581 ggcgccgaga atttgcggct taacgctgcc gtgcttggcc tgacccgcag gaagaccgag
    23641 gaacggtttg acgagatcgt ggagttttcc gaaatcggcg attttatcga cgagccgttg
    23701 cggacgtatt cggccggcat gattatgcgg ctggcattct cggtggccat gaatgtacag
    23761 cccgatattc tgctgatcga cgaggtgctg gcggtagggg accaggcatt tcagcggaag
    23821 tgcatggaag gaatcctgga actgaaacga gccggccgcg ttttgatctg tacgtcccac
    23881 gtcgcggaga cgctgcaaca gctttgtagt cgcgcgatct ggctggacca cgggcgggtg
    23941 attcgggagg gaccggtcac ggaggttctc gacgagtatc agtccatgag gacgggcgcc
    24001 ggcaagcgat gagtggagcc gtggtaccct ttagcgacac gtgaacgcgg acgacattct
    24061 cgggaggatc cgactgatgc tgaatctccc agtgccggag ggacaggagc aacaggcggg
    24121 cgaggacaaa gcggcagcgt ccaagctgct tctagccgaa ctggctgtgg gcgtccggga
    24181 gttgcgccgg cgagcgaatc gcgtgggcct tgttccggag ggctatccgc tgccgctcgc
    24241 catcatcatg aagacgatcc accggctgct cccgtggtac acccgctcca tccaggcaca
    24301 cgcgcaacag gccgcgtcca gcatggatac gattctgatc tgtttgcagg caatcgtgca
    24361 atcgcaggag cgcatcgcgg agcggctcga ccggctggaa cgggaaacgc ggcggccggc
    24421 cgaggccggc tctccgcccc aacagcagtg agcgccgagg ggacagcacg ctccgaacgg
    24481 ttcggcgggc cccccgtcag tgtcgtcatc aacacataca accgcgcggg gcacttgcgc
    24541 cggtgcctcg acgctcttac gaaacagacc taccggaact ttgaagtcgt ggtcgttcaa
    24601 ggtccgtgca ccgatcggac cgccgatgtg ctggcgcact tcggcgacgc aatcacaatc
    24661 gcggcgacgg agacgagaat cctcggcgtc tcgcgaaacg tgggcatcgc gcacagccac
    24721 ggagagctga ttgcgttcac tgacgacgac gccgttccgg atcccggctg gctggcgaac
    24781 ctcgtgcccg ccttcgatga tccggaagtc ggcgccgccg gcgggatggt gtaccgcatg
    24841 ggcgatggca gcatagagtg gcgtaacggc attctcgacc gggacggtat gctcgatcct
    24901 attcggccgg agcccggccc gtttccaagg cgcagggacg ggaggctgaa caccgtttcg
    24961 ggtaactgct gcatgtttcg ccgcgaggct ctggaagaga acggtggctt cgatgaagcc
    25021 atcgttttca tgtacgacga aagcgacatg gtgcagcgaa tggagctctc gggttacagc
    25081 gttatccacc ggccggacgc tgtcgtgttt catgaatcgg cacgctccga gatgcgggag
    25141 aaccgcctcg attacaactg gttcgtcatc tgcaagtacc acgcttacgg agcactgaag
    25201 aacgcacgcg cgccccactc gtgggccgcg gtacgaatcg cgtggcggct catagaccgg
    25261 cggatgctgg tcaatctgcg ctggtggggg cggagactga tcggcagccg tcagctgctc
    25321 cggttcgaga gccaatgtct gcgcgggatt gccgctggac tatggaaggc ctggaatcgg
    25381 ccggcggcgg tacaaacgcg tctgccggcg gcggagactt ctcccatccg tccgttttgc
    25441 tacacgccgg cgcgccgttt gcgcgtatgc ctgctaagcc agtcgacacc ggagcgttct
    25501 cccgatggca tcgccacata cacgttcacg ctcgccaggg cgctgtttga gcaaggctgc
    25561 gacgtccatg tgatcagccg tggagaggag gaacgctcct cgctttccga gggcatctgg
    25621 cgacacgagg tgacgcccga gaatatctct gcaagactcg gtctcggatc cgaattcccc
    25681 gccacggccg aaaaccttga ttacgcgaac gccgcatggc ggaaaatccg cgaactggat
    25741 gagcggaacg gtctcgacct cgtcgaggcc tcttcatgga actctgaagg actcatcgct
    25801 gcgctcgacg ggagaatccc cgttgtgatc cacgttcatt catccctgct tcacgtcatg
    25861 gagacgcaag agtgggagga gaacgccgac cggtggctgt gcgcggaact cgaggagcgg
    25921 cagagcctgt tggccgacgg cgtggtcacc agtacccggg aggttgcgca gtccgtattc
    25981 agccactaca aacgccggcc ggttctcacg gctgtcattc ctctcggcct ggagatcccc
    26041 gctcttcgcc cggcggcgcg agaggacagg atgaagaaag ttctgtttgt cgggcggctg
    26101 gagcctagaa agggaatcgg agtgctgctc gaagcgattc cgagggtgct cgaggccgtt
    26161 cccgaagcgg aattcgacat cgtcggcaag gatgtgatgg gcgccagttc gccggtgacg
    26221 cgctggtggc aggcgcatcg ctcgctgaga cgccgggtgc gatttcacgg ggaggcgccg
    26281 tcgcgggcgc tggacactct gtacgagaca tgcgatctgc tggtggcgcc gtcacggtat
    26341 gaatcgttcg gcctgatcta cgtcgaggcg atggcgcgag gtaaagcggt gatcgggacg
    26401 acggcgggcg gaatcccgga ggtggtccgt gacgcggaaa cgggtctgct cgtgccgccg
    26461 gatgatcccg aggcgctcgc cgctgcgatc atccggctgc tccgcgacga cgagtacagg
    26521 aggagccttg ggcaagcagg gtacggacga taccgggcgg aattctcggc gtggaagatg
    26581 gctgggcgga tgctggattt ctaccgtgaa gtgatcgctc gaagagcggc tcaaactccg
    26641 agcgacgcca acagatcgga aagccgttgt tcggctgcgt cggaagaata gtacgcggcc
    26701 gcttcgcgct gggcgcgcag gattctttct ttccagacag gatcggacac gaccacaccg
    26761 atcgcctccg ccatctccgg atggtttttc tctgaaaaca ggatcccagc cccgcgcacc
    26821 gtctcgggca ctgcgcagga ggcgtacgcg aagaccggca cgtcgaagag cattgcttcg
    26881 atcagcggca cgcagaatcc ctcgtgctcg ctcatcgcta agaaggcgtc ggccagacgg
    26941 tagtacgcgt tgagcgccgc ctggctaacc cggccggtga atactacgct gccgtcgagg
    27001 ccgagccgtg tcaccagctg ctggaggcgc ataaggtagt actctgtgct ggtggcgctc
    27061 ccggcgatga agagcctgct cctgggttcg atccggttac agaaagagtg aaacgccaga
    27121 acgcaatctt cgatgcactt gttcggggcg atacgcccga ccacgagcag gttcacgagc
    27181 ccgtcgccgc ggtagcgcct caagacagac tcgtccggcg gaacatcgtg aagccggtgc
    27241 gtcaacacgt agggaaacac tcggacgttc aggaatccgg catcggtcag atcccgcgcg
    27301 ttgtattccg agtcggcaag cgcaagttcc acgctgcttc ggtagcgcgg tagcccatcc
    27361 cgggccgcca gcacgcgatc ccgcaactcc cctggcatgc cctcgaaaaa agtcggtggc
    27421 gtaacgttgt gatagagcag aacctttcgc gaaggcgcgg cgaagacctg gtcgaagaga
    27481 ttcgagaaga ccgaatagtg aacgatgagg atgttggctt cgcaaccgtg gtactcactc
    27541 cacggttgga cgtaaacgcc ggctgtccgg gatgggacct cagagtaaat cgccgaggag
    27601 agacggcggg cgcggagcat cgccctgaga gtgacgattt cgttgccgac agcgtctccc
    27661 tcgtagaccg ccgtgttgaa ttgatcaacg gcagtgtggc tcacgccccg cgatcctccg
    27721 gcttcgttcg tgaaagcctt tcctcgagga gggagatgcg tgcctcgatc tcggaaatcc
    27781 gcccgagagc ttcgaggcgc agttccacct gcttctcatg aagggcgagc atcatgtgat
    27841 acgcggcgcg ccaggcctgg tcccgccggg aaatcgcccg caccagccac cacaggacgc
    27901 ggtactcaag atcgatcaga aaacggccca agcggccgcg aagcgtaggg ggctcggggg
    27961 ggtatacgcc tccggtttgc tcaatctccg ccacaagctg accgaaacca tacggatcca
    28021 tcccaggctc cgagcggaac gtcgcagcca gccagccgta gtcaatagcc gagccttgga
    28081 aaggccgccg ctccaaacgc cggcgggcga cctctctcag ttccgcaccg agttgttccg
    28141 gagccggagt acccatgatt cgccaatcct aacagaagga atacggtcag cgggaagcag
    28201 aggcctgtcc gcacactcgc tcaaagaacc gccaggatgc ggcagcgcaa tgttcccacc
    28261 ggtagcgctg tgcaagcctg aggccgtggg aggagagctg agttcgcagg ctctcggaga
    28321 gcagcagcgt ctcgatcgcc cgcgcgatct ccgcagtgct ctttggatca gcgagcaacc
    28381 ccgcctctcc ggcaacttcc ggcattgacg aaacgttcga ggtcacaaca ggcgctccgg
    28441 cagccatggc ctgacccacc ggtagtccga atccctcgta aagggaagga tagacgaaga
    28501 gggaggcccc gcgtgtcagg ccaggtagat cttcctccgg cacatagccg agataccgca
    28561 cggtcgagga cggggattgc agccgcgcca ttgtcgacgg ttcggcccat ccaacgggtc
    28621 cggcgagaac gagatcgaat tcggagcgca ggcccgggga aagggtcgcc caggcgtcga
    28681 ggagccggtc gacgttcttc cgcggctcga ttgtgccaac gaagagcgcg taaggccggc
    28741 caaggctgta cttcgccgcc gcaaggccgg ccgatacggg cgttgtccgg aaaaaagcgt
    28801 ccgcgacgcc cggatagatg acctcgatct tttccggcgg caagccgaga atccggaccg
    28861 cgtcccggcg tgtgctctcc gagatggcga tcagaccgtc tgcccggcaa agcacccgtt
    28921 gggcaaaccg atgggccgcg ctgacattgg ctagcgagtg ggtttccggg acgagccagc
    28981 aggtcatgtc gtacaaggtc ccggtgagcc ggccgcggcg gggtggattc caaagctgtt
    29041 gagacgcgtg gaacacgtcg gacttcggcc cgagccaatc gagccacgac accggagagt
    29101 agttgttggc atgaagcaac gccaacctgc cgagcgtggg cagccggcca agcacggaac
    29161 gctcgtggga gtagctgacc agcggaccca gggacgggaa cgtcactact cgaccgtccc
    29221 cagccacacg gcgcagggac acaatccagt tgaataggta tgtcttgacg ccggcgctcc
    29281 gcaacaggag cggaatggca tcgatagcga cacgcatgac tgaacctcat tgtagaagtt
    29341 cggaactcag catgggctgg ttcgtcagaa gaagcttacc cccgctctct gcgaccctgt
    29401 gaaggccatc gcgaccggcg agtctttcga ttctgggcag cgccgtccgg ccggccacca
    29461 ggtagtaccg ctcattccgg gcccagaggt cacggaaacg ctcgtcgtcg atgaacacat
    29521 ccggggcgct gggcgcgttg gagccgtact cgagatttgt cacacgtccg ttgagcagca
    29581 gcgcggtgcg tcccgcatag aagagcaccg aggagaacgc gtagtactgg tcgtcgacga
    29641 tgagcatgcc ttgcggagcg cgccggaggg cttcggcaag cggtttcgac gagaggtacg
    29701 ggtcgaaggt caccattgcg atccgggccg cgtgagcgaa cagcgccatc atgagcgcca
    29761 gcccaaggaa cgcccgcctg ccgctccagc gccaggagca aaccgctccg aggagcagcg
    29821 caatgcccgc caccaccagc ggcaaacgga ggtacgcgaa cgaacggata gtcaggtcgc
    29881 ccatgtgtcc gagcgacagt gtgtagacct cgtaaacgtt ctcgctccgc gccaaggcgt
    29941 ttgaaatgtc cccgggagtg ggcatgccgc gaacaagata cagcaccaca gagatcaccg
    30001 cggctgccgc cgcggtgacg gcggcgaccg cacgcagtcc ccaacggacc cacgcgcctc
    30061 cggccgccat cgccgaaccg agcaacaggg cgaacgccgg gtaagtcggc atcgagtaat
    30121 actcctgggt tgtcgaaaag gtgaagaagg ccaggataaa tccgatccag cacagggcaa
    30181 ggaaacgtgt atggcccgcg cggtcagccg ttttgtagct tagccggaac gtcgccggaa
    30241 gaaacgcgct ccaggggaac agccaaacca agtgcagcaa ccagaagagg tgacgcggca
    30301 cggtattgta gtccctcgga tatcgcatgt tcagaaaccg gaacacgtgc tcgttcagaa
    30361 agtagagcca gaagaagccg tgccagacac ccgggccgct cttcatcgtg agatcgaagt
    30421 acggagggtt tctgagaatc gccaggatgt gccacggagc ggcgatcaac aggatcacgg
    30481 caagtccgga aagcggccgc aagcgctgcc acgtgcgttt tgcgaacaac tgccgcgtga
    30541 gcaggagata gagcccaccg gcgccgagcg gaaacacgat cgcgatgaga cccttgagca
    30601 ggaggcccgc tccaatgctc gccgcgagca acagcgccca cgcccggggg cgcggctcgt
    30661 cttcgtccag agcgcataat aggctccaca tcgacacgca gacggcgagc gtgacgagga
    30721 cgtcggagat cagaattcgc gtgaatagga acaggccggt acaagccgac aggcacagac
    30781 ccgtgtagag tcccgccgtt gcgccaaacg cccacacacc gaatcgatag gccacccagc
    30841 acaacagcac cgtgccgagc gcgagaggaa gccgcgccgc ccagtcatgc acgccgaaca
    30901 ccatatagga aaccgcgatc atccagtact tgagcggcgg tttctcaagg tagaccacac
    30961 cattcaaccg tgcggtgacc cagtcgcccg actcgagcat gttccgcgcg atctgggcct
    31021 gaaccgcgtc cacgtcgtcc atcaacgacg gcgggctggg gatgcagccc aggaaaatga
    31081 cggcggcgat ggcaagaatg atcaaatcac ccgcgccgag cagccgtcgg tcaggaagcg
    31141 atgagccggc tgacggtttc aaaacggaat ccttggtctt tcagttccgg aaggaggcgg
    31201 cggacggctt cgatggtgtt ctcgatatcc ggttcggctt cgacgtcgcg gccgtcgtgc
    31261 aggcagagga tggctccgtt tgcgacgccg cgtcgcaaac agcggagcac ttgatcggca
    31321 gggtacttcc agtcccggcc gatcttcgtc cacattacgc cgagcaaccc taattcctgg
    31381 aggaccccgc gcaggccgaa ccagcgaacg ccgtagggcg gacggaacag caccggcgtc
    31441 tgtccggtgg cttcggcaat cgactcctgc gcccgcctca attcgtcacg caggaacctc
    31501 ctcgaccgca gcgcgagcag ccggtgcgaa tcggtgtgat tgccgatctc atggccggct
    31561 tcagcggtgg cgcgggcgat gtccttgtgt ctccgtgtgt gcatcccgca ctggaaaaat
    31621 gttgcgtggg catcgtgatg ggccagcact tcgagcaggc gagacgtgga accgctcggg
    31681 ccgtcgtcga aggtgagcgc gatcgagcgc ttctcacgag ggccacgata gatccagcca
    31741 ccgaacggat cgagcatcat cattcattct aacggacgag ctcgaaacgc ccgtcgggcc
    31801 gcaggtagta gcggcggccg cgccagagga ttgtgttgcc gacaaaccct ccaagccaga
    31861 cgacgaagct gtagatgtcc tgaagtggca cgctccacca cattctgaga gtgaggcgat
    31921 cccgcagaac ccatcccgcg gtggcgaatg cggcggccac acggaagacc gccgtgaccg
    31981 ggagaagcag ccacaagtcc gggcgcagag tgcagatcaa cagcgcgagc ggaagaggat
    32041 tggtgaatac ttgcccaagg tatcccttgg gccgcgaacg acgggtgctt cgcgcccatc
    32101 gcagccgatg ccgccagttc tccacgaaac ccgtggcgcc gatgtggtgc tcgatcacat
    32161 acgaagagag cagcaccacg taaccggacg cggcgacccg gttgcccatg acgaagtctt
    32221 ccgcgaggta gtccatcagg ctgttccacc caccgatgcc atcgagcgcc tgccgccgga
    32281 ctgcgatcgt cggcccgagc gcgaacttca tgccctccat catgcgggct gtcagcacgc
    32341 cgccgagaaa ctcggtattc atgccgacgg cttccagcgc ggaccagaaa ctctgtccag
    32401 gcaccgcgcg gtacggacag gtggccagac cgacggcggg gtcgtcgaac tcggctgcga
    32461 tcgttcggag caggcgcgcg gtcacgcgga tatcgctgtc gctcatcacg accagatcgt
    32521 gccgcgcggc cgcccacatc atcgacaggc tccagacctt ggcgtttgcg tagggaggtt
    32581 cgccggtcac gatgagccgt gccgcaaccg acgggaactc ccgccggagt cgttctacga
    32641 tggcagccgc gggatcgtcc gcctcgcgga cggcgaagag aagctcatag tcggggtagt
    32701 cctgaaggaa aaatgtgcgg agattttctt cgaggccgag atcggcgccg gagagcggct
    32761 tcagcacgct gatcggcggc atgcgggtag ctgccggagg ccggacagcc agataccgcc
    32821 gggcggcgat cacggtgagc acgcagtaaa cgagcgagcc agccagcacc agcgccaggg
    32881 ccgccgcgac aagcatgatt cactcgtacc gcagggcttc ggcgggggtg atccgggtgg
    32941 cgttgcgcga tgggtagatg gtcgcaagat agctgatgag gatggcggcg gcggcgatcc
    33001 aaacagcgtc aatccagcgg gggtcgaacg gcacgtagct gagcgcgtat acctcttcgt
    33061 ccaggcgcag ccagcggtat cggtcggcga gaaagcagag agtgtagccg gtggcgagcc
    33121 caatgacggt tccgaccgtg ccgatgagga gcccctgcca gacaaaaatg cggcgaatct
    33181 gctcccgccg cgcgcccatc gacgcgagga tggcgatgtc gcggtacttc tccatcacca
    33241 tcatcaccag agcgatcagg atgttcagcg cggccaccag aacgatcagg ccgatcgtca
    33301 cgatggtgac tgtcttctcc atccgtaggg cattgaagat ctgccggttc tgctccatcc
    33361 agctcgtcgc ggccaagtcc ggtcccacca cccgctcagc ggaacgggcc acatcggagg
    33421 ccaggttaac gtcgtcgatt ttcagttcga ccgcgttcac cacgtcgcgc agcgtgaata
    33481 cccgctgtgc cgtcttcaat gatgtgaacg cccaggcgta gtcgatctcg aagaagccgg
    33541 tctcgaatat gcccaccact cggaaccgga agaagctcgg ctttggacca aatggagtca
    33601 gttcgccctg cggtgtgatg acggtggcca cgctattcag cggcaggccg gtgcgggcgg
    33661 acagccgcga gccgagaatg attcctggga ggccggactg atcgtccagc gcttcgagac
    33721 gcccctcctt tagcctccgc agcaggccta ccgtctccga gccggatccg gtctggattc
    33781 ctttcagcac agcaccttcc gactggagag ggctcgacag aaaaacttgg ccgtaaagcg
    33841 tgggcgatgc ggagacgaca tgggggaggc ggcgcaactt cgcgacgagc gccggccact
    33901 cgctgatacc ctcgcccggc tgcttctcga ggaccgttac gtgcgacgtc gctcctagca
    33961 ggctgcgctg gagggtggac cggaacccgt tgttgatggc cagcgcgacc acaagggcca
    34021 tcacgcccgc cgccactccg atcaccgaga tcagcgtgat gacagagatg accacctgct
    34081 tgcgcttcgc ccggagatag cgggaggcga cgaagaactc gaaccgcccc cgcctccgtt
    34141 catcaggcat cgaggtcgcc caagcggtcg gagagtccga tcggtccctc cggccgcagc
    34201 aacgggaaga ggatcacgtc ccggatagac ttcgagttgg tgaggatcat ggcgatgcgg
    34261 tcgataccga tcccttcgcc cccggtggga ggcattccgt aacatagggc gcggacgaaa
    34321 tcctcgtcca tctggtgggc ctcttcgtcg ccgctttccc gcattttgac ctgcatctcg
    34381 aagcggcggc gctgctccag tggatcgttc agctcggtat aggcgttgcc gagttccatg
    34441 cccgcggcgt aaatttcaaa ccgctcgacc atgccgggct cgtcgggctt gttcttcgac
    34501 agcggcgagg tctcgaccgg gtagtcgtag atgatcgtcg gctggatgag atgcgactcc
    34561 gcgattcgct cgaacaggtc caccaacgcc tcgcccgccg agggcttgcc ggagcgcttg
    34621 cgcagccatt cgggatcgcg gagatcgtcc atcgaaggcc gttcaccatc cttccagaac
    34681 tcgatgatcg cctcgcgcat cgagaaccgc cgaagccggg agaagtcgat cttagtcccc
    34741 tggtagtcga cctcgaccga tccggtagcg tcgacggtca cctgccgcag caattcctcg
    34801 gaaaagtcca tcagaccccg gtagtcggta taagcctgat agaactcgag catcgtgaac
    34861 tccggattgt gctgggtgga gataccctcg ttgcggaagt tccggttgat ctcgtaaacg
    34921 cgctccaggc cgcccaccac gagccgcttc aggtagagct cgggcgcgat gcgcagatac
    34981 agatcaatgt ccagtgtatt gtgatgcgtg acgaacggcc gggcggttgc gccgccgtaa
    35041 agaggctgca tcattggcgt ctcgacttcg atgaaaccgc gctcctcgag ccgccggcgg
    35101 aatgatgaca cgatgcgcgc ccgagtaacg aaagcttttc gcacttcggg gttggcgatg
    35161 aggtcgaggt atcgttgccg gtagcgcacc tccacgtctt ccagtccatg ccacttttcg
    35221 ggcatagaca ggagagtctt ggccagatgc acaaggcgct cggcatggat ggaaagctcg
    35281 ccggtccgcg tgcggaaaat gtatccctcg acgccgatga tgtcgccgat gtcgagattc
    35341 tgatagagcg agaagtcgcg ttcggacact gcgtccttac ggatgtagat ctggatcctc
    35401 tcgccgtttt gcatcagatg cacaaagccg gccttgccca tgcggcgaac ggtctgtacg
    35461 cgtccgcaga cgcggacgtt catcttgtcg gagagctgtt cttcggtctt cggaccccaa
    35521 atcgccagga cctcgggaat ggtgtgtgtg aagtcgaaac ggctgccgta cgggcggaag
    35581 ccaagcgctt cgatctcacg gatgcgcttg agccgctgct cgagcagttc ctgttccagt
    35641 gacaatggga tctcctaggc ggaaatcacc tattataatt gagcgttgaa atccatctcc
    35701 atcgtcattc ccgcctataa cgaagagaat cggttgccgg cgacgctcga cagggtgctc
    35761 gagtacctgg attcgagaca ttttccgccg agcgaggtga tcgtggtgga cgacggttcg
    35821 accgatggca ccgcggtggc cgccgtacgt tacggggaaa gcgattcccg cgtgcggctg
    35881 ctgaggaatc cgggcaatcg cggcaagggg tacagtgtgc gccatggcat gctggaagcc
    35941 gaaggagagt ggcggttgtt cacggacgcc gatctttccg ctccgatcga ggaactggac
    36001 aagctcctgg cggcagcgag ggatagccgg gcgtcggtgg tgatcggttc ccgtgcgctc
    36061 aaccgctcgc tgatccaggt acatcagtca ttcttccgcg agaccgccgg gcgcgtgttc
    36121 aacctgttga tgcgattgct caccgggttg ccgttctggg acacccagtg cggtttcaag
    36181 ctcttcgacg cccacgcggc tcgcgaggtt tttcgccggc agcgcatcga gggttttggg
    36241 ttcgacgtcg aagtgctgtt tatcgccagg aaactgcgat ttcacgccgt cgaagtgccg
    36301 gtgcggtgga gccacgttga gggcaccaag gtgcggatgc tcaaggacag catgggtatg
    36361 tttgccgatc tcgcctgcgt gaggtggaat cagctgcgcg ggctttaccg gta
//
LOCUS       DATIFQ010000448        13961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1108628, whole genome shotgun sequence.
ACCESSION   DATIFQ010000448 DATIFQ010000000
VERSION     DATIFQ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13961
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108628"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..352)
                     /locus_tag="VLE22_22390"
     CDS             complement(161..352)
                     /locus_tag="VLE22_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17217.1"
                     /translation="MSFFDTCDADDHWFPDLDAVNGSLTASGTANLWTWRACLFLSSE
                     WSDGIIGQTINVDGGAVMK"
     gene            400..1170
                     /locus_tag="VLE22_22395"
     CDS             400..1170
                     /locus_tag="VLE22_22395"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSB17218.1"
                     /translation="MTGRTISHYQILEKLGEGGMGVVYKARDTHLDRFVAIKVLPPEK
                     VADPERKRRFVQEAKAASALSHPNIIHVYDIDQADGIDYIAMEYVAGKRLDRLIPRHG
                     MPLSEALKCGVQIADALAAAHGAGIVHRDLKPGNVMVTDKGQVKVLDFGLAKLTETGE
                     SGEDPVTRTIRDEEAPRTGEGRILGTAAYMSPEQAQGKKVDARSDIFSFGSLLYEMVT
                     GRRAFQGDSVVSTLAAILKENPAPPSQVVEGLRQNGSA"
     gene            complement(1192..1932)
                     /locus_tag="VLE22_22400"
     CDS             complement(1192..1932)
                     /locus_tag="VLE22_22400"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSB17219.1"
                     /translation="MDSDTIGYMPRRDRCILVGVPCHVTQRGVDRRETFSADADRETY
                     LGLLRRNLQDARVALLAWCLMTNHVHLITLPERVDSLSVLLRRVHGRYAQYYNARYGR
                     SGHLWQNRYFACSLGPSHLWAALAYVERNPVRAGLVEWPGAYPWSSAAAHLEGRDPSG
                     LVDLEWWRREYDGLDWGRSLVGSDGEQSVQLRRWTYAGRPFGDDQFVKSLSEQFGRRW
                     ARGRPKKERATASAVEVASRLELFAEHT"
     gene            2120..3091
                     /locus_tag="VLE22_22405"
     CDS             2120..3091
                     /locus_tag="VLE22_22405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17220.1"
                     /translation="MSGKVLMEDGSSPPEQVTVEKVCGGGVPEPVARTDPKGGFVVRS
                     RSELNTVDASQRSETRSARGSAAPTVIPAGCSVRARLAGYQSSALMVVNPKAVDLGTI
                     ILRPTSGSEGTTVSLTSLKAPKNALKAYDKAMKALEKKKFDEARVELEKATTSYPEYS
                     EAWLELGRVRQYSRELGPAREAYEQAIKADPKFVKPYVQLATVLNAERKYEQLVSVTA
                     TAIKLDPVSFPAAHLLNAMGNLRLGDMKAAAASARQAVKLDPEHAFPEAEYTLGVALG
                     NLGDYQGAAEHLRTYLQVAPNSPSAAAVKQQLAEFEQLAKTAPAAPR"
     gene            complement(3063..3557)
                     /locus_tag="VLE22_22410"
     CDS             complement(3063..3557)
                     /locus_tag="VLE22_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17221.1"
                     /translation="MNLECSCTLVLNQPYDQARRRILAALKEQGLQVAFDFDVASGIL
                     RSSGVRLPKCSVLGVGCPYQLLEAFVADAAAALYFPLHVVLSEHGAETQVRILAPQTL
                     RAAGVTPAISIPVHRTLRRIRDALALVGTRSTKCTQEGPPPLDTEAADETLQTTSAGR
                     QEPS"
     gene            3722..4765
                     /locus_tag="VLE22_22415"
     CDS             3722..4765
                     /locus_tag="VLE22_22415"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HSB17222.1"
                     /translation="MFLLTLLLAAQIAPQSADLPNRQPQLATDGIRLALTYGAGDRVF
                     FAESKDAGKSWSKPVVVYSQGKLSLGMRRGPRIAMTPQVIVISAVVGKQGRGADGDLT
                     AWRSVDGGKTWSAGRAINDVPSAAREGLHTMAAGGKGILFAAWLDLRGKGTRLYGSTS
                     SDGGATWSPNTLVYESPSGTVCECCHPTAAIDAHGSIFVMFRNSIEGNRDMYLVRSDD
                     GGKTFGRAAKLGAGTWKLNACPMDGGSLQLGADGKPISTWRREGEVFYSTGPDPEQRI
                     GTGKHPVLATTPRGPVLAWIEGKALKVRAPGQRDATTLDSDAAYPSMVALPTGTVVLA
                     WERGGTIVVKGLD"
     gene            complement(4806..5351)
                     /locus_tag="VLE22_22420"
     CDS             complement(4806..5351)
                     /locus_tag="VLE22_22420"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB17223.1"
                     /translation="MTRKQFLLSVPLAASVMRGANETRKAPELVLTDTNGKQIRLSLL
                     RGKVVVLEFMLTTCPHCRDTARLLSRLQEEYGREGFQAISLPFNDDAVTAAPRFVADY
                     QVTHPVAPIQRERAYKFAQLSAGILHTVPIVVFIDRRGRIRAQFEGKDGFFRNEEANF
                     RATIFELLKEPAPSPSGGHAH"
     gene            complement(5368..6738)
                     /locus_tag="VLE22_22425"
     CDS             complement(5368..6738)
                     /locus_tag="VLE22_22425"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB17224.1"
                     /translation="MIGKRILVVDDEENLRRVTQLKLQQAGYEAMTASDGVEALGLLA
                     RHPQDLVLTDLKMPGMSGIDLLRKVKEEYAEVIVVVVTAYGTIESAVEAMRLGAYDYI
                     IKPVNSDALNLIVSRALEHHRLQEEVKNLRSAIDRKFGFENIIGQSKNLLTTLDNASR
                     AAQSDSTVLIRGETGTGKELLAKAIHFNSPRRDRPFLVINCGAIPKELLESELFGHTK
                     GSFTGALANKVGKIELADQGSLFLDEIGEMPWELQVKLLRLIQEREIEKIGAAAPTKV
                     NVRIIAATHRNLQAMVEDGTFREDLYYRLNVIPLTLPPLRERPDDIPELVAIFFEKFK
                     ERLNRPGLRLPPALLPYFSAYRWPGNVRELENVVERLLVLAPGDEVTVADLPDFLVRE
                     RPAMEAIQLELPPQGISIEAVERELVLRALQRFAWNQTKAAEFLDISRKMLIYRMEKF
                     NLRKED"
     gene            complement(6735..7922)
                     /locus_tag="VLE22_22430"
     CDS             complement(6735..7922)
                     /locus_tag="VLE22_22430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB17225.1"
                     /translation="MTTQPQARLKSPSPHSRSHGDASSPDEVTLSIDAERTAWNWKAV
                     ALVLAGIALTSLGHYLTPRDFRLWHGVFQRLYYLPVVYAAIAFGWVGGLAAALLAGIL
                     YIPHIVTTWQHEHHYAMEQYAEIFMFLAVGIVTGVLSDRERKRSAELQLTAQKLSHVY
                     RELQDTFEQVKRADRLSAIGQLAAGLAHEIRNPLASIDGAAEVLDTAGDRPELRKETA
                     SIIRKECSRLNRLLTSLLDFARPRRPEWREVDLSRVLDSVIDLVSHSAGKGIRFHKET
                     VRGIPRLLGDEEQLTQVVMNLTLNAAQAMPEGGDVWLTTKQEDDGIEIRIRDQGAGIP
                     EESMDKIFDPFFTTKDSGTGLGLSVVHQIVSQHGGTVTVTRNQDKGMTFSLFFPQSRG
                     DLA"
     gene            complement(7861..8688)
                     /locus_tag="VLE22_22435"
     CDS             complement(7861..8688)
                     /locus_tag="VLE22_22435"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSB17226.1"
                     /translation="MNTRRRVIARLAPLLLLLATVPTARLFAVEGIPHWELVLNDVLE
                     RAEQERTSKDAGSPRAQSARNPNPSVKSGERALRPIVGDFVRYFQGPGLGHYRASVKR
                     LRAYHPMITRVLREEGLPPELLWVGLVESGYDPRARSPKNALGIWQLIPETATTFGLT
                     VARRDERTDPEKSTRAAARYLKSLYARFGDWALALAAYNAGENRVQSAIERAGDRDFW
                     RLSASGMLPRETQAYVPAVMAAQFLGEGSAVEDRSSVYDDATPGTAKVALAPFSLSR"
     gene            8992..10248
                     /locus_tag="VLE22_22440"
     CDS             8992..10248
                     /locus_tag="VLE22_22440"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB17227.1"
                     /translation="MRSPLMGRALLLVPFFVSGLWAQADRAELKDLLAEALSSNPEII
                     AAQKRYEASRQRPTQASSLPDPMFSPSYNSNGRPWPGAGLGMEPTSQIGFMVSQEIPF
                     PGKRKLAGDMAVKESEAEWQQYQQIQLSVVSRLKQAYYRRAYAFAAVDVLDRNLELLR
                     KFLRITEARYAVGKAAQQDVFKAQTQISILETKRVQLEREKRAREAEIASLVNRPPGS
                     QLPRPADLRPIPVPVKLEELYAAARENSPMLRRDEKMIQRAEVAVNMARKEYYPDFTL
                     NGGYYNMGRMPDMYMFRADFKIPLYFFRKQRAAVTEQAQTLVESRKTYEATNQSLHFR
                     IQDDYLMTQTSEQLVRLYGQTVIPQASLALESSLSSYETGSVDFLTVLMNHITVVEYE
                     MNYYEELQNFYLALARLEEMTARPLT"
     gene            10270..11736
                     /locus_tag="VLE22_22445"
     CDS             10270..11736
                     /locus_tag="VLE22_22445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB17228.1"
                     /translation="MKPARIILFVALVAVGILIGFGYGRWYGPRQAAGVTAQVKKPTG
                     YHCPMHPSYRSDKPGDCPICGMKLVPDDEPEHPGHAPAQASAERKILYYRDPKNPDFK
                     SDKPGLNPETGNELEPVYADEPASMPMGTIRVSPQKQQLIGVKYGEATSAAGLHTFRA
                     VGKVAMDETRVAKVQTKIEGWIEKVYVDFTGKVVEKGEPLLTLYSPEMLASQQEYLLA
                     IRSREVMQSSPLAGTQQQSESLLDASRKRLELWDLSDAQIEEITRTGKPLKNITLYSP
                     ISGYVMMRNAFPKQRITPETELYTVADLSKVWIMADVFENETPMIRVGMPVRVSLSYA
                     GGRKLQGRVDYIQPQVDMTTRTLKVRIEAENPAMVLKPEMFVDVEFQVAMPASVTVPS
                     EAVLNTGLRQTVFVDRGNGYLEPRQVETGERIGNRIEITKGLQAGERVVISGNFLIDS
                     ESQLKSAAAGMAGHQHGGATPKPAELKPSTPVGEHQHD"
     gene            11729..>13961
                     /locus_tag="VLE22_22450"
     CDS             11729..>13961
                     /locus_tag="VLE22_22450"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB17229.1"
                     /translation="MIDQIIEFSGRNKFLVFVLIGFAVVAGLWSMTQVPLDAIPDLSD
                     TQVIVYSRWDRSPDIMEDQVTYPIVTTMLGAPKVKDVRGFSDFGYSFVYIIFEEGTDI
                     YWARSRTLEYLSGVLPRLPQGVKTELGPDASGVGWVFQYALVDESGKHSLAELRSLQD
                     WFLRYQLKSVPGVAEVAPLGGFVRQYQVNVDPNRLQAYNVPIMKVVEAVRNGNNDVGG
                     RLLEFSGTEYMVRGRGYAKSTDDIGNIVLLSTESGVPVRVKDVGDVVLGPDIRRGIAD
                     WNGQGDVVSGIVVMRQGENALQVIERVKAKIKDLEPGLPEGVKIVSAYDRSELILRSM
                     ENLKSTLTEEMVVVAVIIFIFLWHIPSSIIPVFTIPIAIIISFIPMKLMGVSANIMSL
                     GGIAIAIGAMVDAAIVVVEQTHKHLEEWDRTGRQKDYHRVVIDAVKEVGGPSFFALLV
                     IAVSFLPVLTLEAQEGRLFKPLAYTKNLSMIIAAVLAITLDPAMRLLFTHMDNFNFRP
                     RWLARVTNAVLVGKIHSEENHPISKILIRIYDPVCRWSLRWKYAVIAGAVATVFATIP
                     IYEKIGSEFMPPLDEGTLLYMPSTLPGISVTEAERLMQIQDRIIKGFPEVLTVLGKSG
                     RAETSTDPAPLSMMETTIVLKPHDQWRKADTWYSEWTWAPSWLTAVFRHITPDHIPTE
                     QLVDEMNEALTLPGVSNAWTMPIKARTDMLTTGVRTPVGIKVYGANLKEIERIGTEIE
                     GLLP"
BASE COUNT         2786 a         4243 c         4256 g         2676 t
ORIGIN      
        1 gcaaatggaa gtcgctcgaa gcggcgctgc gcggaatgtt ctggtcgcaa cgactcccca
       61 ttgacgaacg gccatgacga tgcaccggtg agcgaggtaa cccggggccg cttcggtccc
      121 gggttgtttt tatgtgcgtc ccgcagctca ctggttggaa ctacttcatc actgccccgc
      181 catccacatt gatcgtctgc ccgatgatcc cgtcgctcca ttcgctcgac agaaaaagac
      241 acgcgcgcca cgtccagagg ttcgccgtgc cgctcgccgt cagagaaccg ttcacagcgt
      301 cgagatctgg aaaccagtgg tcgtccgcgt cgcacgtgtc aaaaaagctc aaccctccaa
      361 ctcgcgggcc gggattatac tccgaaagga gggccgccca tgaccggccg caccatctcg
      421 cactaccaga tcctcgagaa gctgggcgag ggcgggatgg gtgtggttta taaggcgcgg
      481 gacacccacc tcgaccgttt cgtcgcgatc aaggtcctgc caccggagaa ggtcgccgat
      541 cccgagcgca agcggcgatt cgtacaggaa gccaaggcag cctcggcgct cagtcatccc
      601 aacatcatcc acgtctacga cattgatcag gccgacggta tcgactacat cgccatggag
      661 tatgtcgccg gcaagaggct ggaccggctg atcccccggc acgggatgcc gttgagcgaa
      721 gcactcaagt gcggcgtgca gatcgccgat gcgctggcgg ccgctcacgg agcgggcatc
      781 gtgcaccgcg acttgaagcc gggcaacgtc atggtgacgg acaagggtca ggtcaaggta
      841 ctcgacttcg ggttggccaa gctgacggag acgggggaaa gcggcgaaga tcctgtaacg
      901 cgcaccatcc gggacgagga agcgccgcgc accggagaag gcagaatcct gggcacggcc
      961 gcctatatgt cgccggagca ggcgcaaggc aagaaggtcg acgccaggtc ggacatcttc
     1021 tcgttcggct ccttgctgta tgagatggtg accggccggc gggcgtttca gggtgactcg
     1081 gtggtctcga cactggcggc catcctaaag gagaatccgg cgccgcccag ccaggtggta
     1141 gaaggtcttc ggcaaaacgg gtcagcctga actgcgcgat tttttccgcg ctcacgtgtg
     1201 ctcggcaaac agttccagtc ggctcgcgac ttccacggcg gaggcagtag cgcgttcttt
     1261 cttgggccgt cccctcgccc aacggcgacc gaactgctct gatagcgact taacgaactg
     1321 gtcatcgcca aacggacgcc ccgcgtaagt ccagcgcctc agttgcaccg attgctcgcc
     1381 gtccgagccg accaggctcc gcccccaatc caatccatcg tactctcttc gccaccactc
     1441 cagatccaca agcccactcg ggtcacgtcc ttccagatgc gcggcagcgc ttgaccaggg
     1501 atatgcgccg ggccattcca ccaacccggc ccgcaccgga ttccgctcca catacgccaa
     1561 ggccgcccag aggtgacttg gccccaacga acaggcaaag tatcggttct gccagagatg
     1621 accgcttcgc ccgtaccgcg cgttgtaata ctgtgcatac cggccgtgga cccgccgcag
     1681 aagcacggaa agagagtcca cccgctccgg caaggtgatc agatgaacat ggttcgtcat
     1741 cagacaccaa gccagcaggg cgaccctggc gtcttggagg tttcgccgca gcaaccccag
     1801 gtaggtttcg cggtcggcat cggcagagaa cgtctcccgc cggtcgaccc cacgctgcgt
     1861 cacatggcaa gggacgccaa ctagaataca gcggtcacgc cggggcatat acccgatagt
     1921 gtcgctatcc atccggggct ctcaaataaa tcacgcagtt caggctgacg cgtttttgga
     1981 cgcgtttttg cggacgggtt tttgagtgta taatcttgcc gtgtcctttc ggttcggatg
     2041 cgtcctcatc gcggttgtgg tcccgctcgc cgctcagcgg ggtaatccag ttcccggcca
     2101 tatggagatc cccactcaga tgtccgggaa ggtcttgatg gaggatggca gctcgcctcc
     2161 ggaacaggtg accgtggaga aggtctgcgg cggcggcgtt cccgagcccg tcgcgcgtac
     2221 cgacccgaag ggcggtttcg tggtccggtc gcgctccgag ctcaacaccg tcgatgcgag
     2281 tcagaggagt gaaactcgca gcgcgagggg gagcgccgcg cccaccgtaa tcccggccgg
     2341 atgctcggtc cgggcccggc tcgccggata ccagtcgagc gccctcatgg tcgtcaatcc
     2401 caaggccgtc gatctcggca cgatcatcct ccggccgacc tcgggcagtg aaggaaccac
     2461 ggtaagcctt acgagtttga aggcgcccaa gaatgcgcta aaagcgtatg acaaggcgat
     2521 gaaggcgctc gaaaagaaga agtttgacga ggcccgcgtc gagctcgaga aagcgaccac
     2581 gtcctacccc gaatattccg aggcatggtt ggaactgggg cgagtccgcc aatattccag
     2641 ggaactcggt ccagcccgcg aggcctatga acaggcgatc aaggccgacc caaaattcgt
     2701 caagccgtac gtccaactgg cgacggttct caacgccgaa cggaagtacg agcagttggt
     2761 gagcgtcacc gccaccgcca tcaagctgga cccggtgagt ttccccgccg cgcatctcct
     2821 caatgcgatg ggcaaccttc gccttgggga catgaaagcg gccgcggcaa gcgcccggca
     2881 ggcagtcaag ctggaccccg agcacgcctt cccggaagcc gaatacacac tgggcgtcgc
     2941 cctcggcaac ctgggcgatt atcagggtgc ggccgaacac ctgcgaacct atcttcaggt
     3001 ggcgccaaat tcaccgagcg ccgctgcggt gaagcagcaa ctggccgagt tcgagcaact
     3061 ggctaagacg gctcctgccg ccccgcgcta gtcgtttgaa gagtttcgtc cgccgcttcc
     3121 gtgtccagag gcggggggcc ctcctgcgtg cactttgtgg atcgggttcc aaccagggcc
     3181 agggcgtcac ggatgcgtct cagcgtgcgg tgaacgggga tcgatatggc tggggtgaca
     3241 cctgctgccc gaagagtctg tggcgcgagg atgcggacct gggtttccgc tccgtgctcg
     3301 gagagcacaa catgtagagg gaaataaagc gccgccgcgg cgtcggcgac gaacgcctcc
     3361 aggagttggt acggacaacc cacgcccagc accgagcatt tcggcaacct cactccggat
     3421 gagcgcaaga tgccgctggc tacgtcgaag tcgaatgcca cctggaggcc ttgctccttc
     3481 aacgccgcca gaatccgcct ccgcgcctgg tcgtagggtt gattcagaac cagggtgcaa
     3541 ctgcattcga gattcattgg cgtccccgga atttgtccat gcaatctgat cgccaagctc
     3601 gtagccggcg tctttcggcg tgattctgga ggatcttgga catctggtgt gcggtattgg
     3661 ccaacggggg ccagaactca tcgtgcctat aatgaaagaa aggttcaaag ctgggtagac
     3721 gatgttcctc ctgactcttc tccttgcagc gcagattgcg ccacagtccg ccgacttacc
     3781 gaatcgtcag ccgcaactcg ctaccgatgg aatccgcctg gcgttgacct acggggccgg
     3841 cgaccgtgtt ttcttcgcgg agtccaagga cgctggcaaa agttggagca agccggtggt
     3901 cgtctactcg caaggcaaac tctcactcgg catgaggaga gggccgagga tcgcgatgac
     3961 gccacaggtc attgtcatct ccgcggtggt tgggaagcag ggccggggcg ctgacggcga
     4021 tctgaccgcc tggcgttccg tggacggtgg aaagacgtgg tccgccggaa gggcgatcaa
     4081 cgatgtgcca tcggcggccc gagagggatt gcataccatg gctgctggcg gtaagggcat
     4141 tctattcgcc gcctggctcg acctgcgcgg aaagggtacc cgcttgtatg gctcgacctc
     4201 atcggacggg ggcgccacct ggtcgccgaa cactctggtc tacgaatccc cgtccgggac
     4261 ggtgtgcgag tgttgccacc cgacggccgc catcgatgcg catggaagta tcttcgttat
     4321 gtttcgcaac tcgatcgagg gaaaccggga tatgtacctg gtccgctccg atgacggtgg
     4381 aaagactttt ggccgcgcgg ctaagctggg cgctggtacc tggaagctga acgcgtgtcc
     4441 catggatggc gggtctcttc aactcggggc ggacggtaaa cccatctcga cctggcggcg
     4501 tgagggggaa gtgttctatt cgacgggtcc cgatccggag caacggattg ggacggggaa
     4561 gcatcccgtc ctggctacga cacctcgcgg gcccgtcctg gcctggatcg agggcaaggc
     4621 gttgaaggtg agggcgccgg gacagagaga cgccaccact ctcgacagtg atgccgccta
     4681 cccgagcatg gtggctttgc cgacagggac tgtcgtgctg gcctgggaac ggggcggcac
     4741 aatcgtcgtc aaaggactgg actgaatcgc gacttagccc ggctcttccg gctttgtgtt
     4801 cccggctagt gagcgtgtcc gccgctcggg ctcggagccg gttccttcag caattcaaaa
     4861 atcgtcgccc ggaagttcgc ttcttcgttc cggaagaagc catctttccc ctcgaactgc
     4921 gctcgaatcc gcccccgcct gtcaatgaaa accacgatcg gcacggtatg cagtattccc
     4981 gctgacaact gggcaaactt gtaggcccgt tccctttgga ttggagccac cggatgagtt
     5041 acctgatagt cagccacaaa tcgcggcgcc gctgtaaccg catcgtcgtt gaacggaagc
     5101 gagatggcct ggaagccctc tcggccatac tcctcttgta gccggctcag cagccttgcg
     5161 gtgtcccggc agtggggaca cgtcgtcaac atgaattcga gaacgaccac tttgccgcgc
     5221 agaagactca agcgaatctg cttcccgttg gtatcggtga gcactagctc gggagccttt
     5281 cgggtctcgt tggcgccgcg catcaccgac gcggcgagcg ggacgctcag gagaaattgt
     5341 ttccgggtca tcgactgtct caaactttca gtcctccttg cgaaggttga acttctccat
     5401 gcgatagatc agcatcttgc ggctgatgtc gaggaattcg gccgccttcg tctggttcca
     5461 cgcgaaacgc tggagcgccc ggagcacgag ttcgcgctcc accgcctcga tgctgattcc
     5521 ctgcggaggc agctccagtt ggatcgcctc catcgccggc cgttcgcgca cgaggaagtc
     5581 cggcagatcg gcgaccgtca cctcatcgcc aggcgccagg acgagaagac gctccacgac
     5641 attctcgagt tcgcgcacat tcccgggcca ccgataggcc gagaagtacg ggagtaaagc
     5701 gggtggcagt ctcaggcccg ggcggttcag acgttccttg aatttctcga agaagatggc
     5761 gaccagttcg gggatgtcgt caggccgctc ccgcagaggg gggagcgtca gaggaatcac
     5821 gttgagacga tagtagagat cctcccggaa cgtgccgtct tccaccattg cctggagatt
     5881 ccggtgggtc gcggcgatga tgcggacgtt gactttggtc ggagccgccg ctccaatctt
     5941 ttcgatctcg cgctcctgga tcagccgaag aagcttgacc tgcaactccc agggcatctc
     6001 gccgatctcg tccaggaaca gcgatccctg atcggccaat tctatcttgc ccaccttgtt
     6061 cgccagtgcg ccggtgaacg atccctttgt gtggccgaac agttcagact ccagcaactc
     6121 cttcgggatc gcgccgcaat tgatcacgag gaagggccgg tcgcggcgcg gactgttgaa
     6181 gtggatggct ttcgccagga gctccttgcc cgttccggtt tctccgcgaa tcagcacggt
     6241 ggagtcgctc tgcgcggcac gcgacgcatt gtcgagcgtc gtcagcaggt tcttggactg
     6301 gccgatgata ttctcgaagc cgaattttct gtcaatggca ctgcggaggt tcttgacctc
     6361 ctcctggagg cgatggtgct ccagcgcccg tgacacgatc agattcagcg catcggagtt
     6421 caccggcttg atgatgtagt catatgcccc caagcgcatc gcttccacgg cggactcgat
     6481 ggtgccatat gcggtgacca ccacgacgat cacctcggcg tactcctcct taaccttccg
     6541 gagcaggtcg atgccggaca tcccgggcat cttgaggtcg gtgagaacga ggtcttgggg
     6601 atgccttgcg agcagcccca gagcttcgac tccatcgctc gcggtcatcg cctcgtagcc
     6661 ggcttgctgt agtttcagtt gagtgacgcg gcggagattc tcttcatcgt cgacaaccag
     6721 gattctcttg ccaatcatgc gaggtcgcct cgcgactgcg gaaagaacag actgaacgtc
     6781 atgcccttgt cctggttcct ggtcacagtg acggtcccgc catgctgcga gacaatctga
     6841 tggaccacgg aaaggccaag cccggtccct gagtcttttg tcgtgaagaa cgggtcgaag
     6901 atcttgtcca tgctctcttc gggaattccg gcgccctggt ccctgatccg gatctcgatg
     6961 ccatcgtcct cttgctttgt tgtcagccag acatctccgc cttccggcat cgcttgcgcg
     7021 gcgttgagag tcaggttcat caccacttgg gtgagttgtt cctcatcgcc gagaagcctt
     7081 gggataccac gaaccgtctc cttatggaaa cggatgcctt taccggcgga gtggcttacc
     7141 aagtcgatca cggagtcaag aaccctggat aaatccacct cacgccactc ggggcggcgg
     7201 ggccgcgcga aatcgagcag gctcgtcagg aggcggttca ggcgcgaaca ctccttgcgg
     7261 atgatgctcg ccgtctcttt ccgcaactcg gggcgatcgc cagccgtatc cagcacctcg
     7321 gctgccccat cgatgctggc gagtggattg cgaatctcgt gcgccaggcc ggcggccagt
     7381 tgaccgatag ccgagaggcg gtcggctcgt ttgacctgtt cgaaggtgtc ctggagttcc
     7441 cggtatacgt gactcagctt ttgcgcggtg agctgcagct cggcgctccg ctttcgttcc
     7501 cggtccgaca acacgccagt gacgattccg accgcgagga acatgaagat ctccgcgtac
     7561 tgttccatcg cgtagtgatg ctcatgctgc caggtggtca cgatgtgggg gatgtacaga
     7621 atgccggcca gcaaggcagc cgcaagcccg ccgacccagc caaatgcgat tgcagcatag
     7681 acgacaggga ggtagtagag acgctggaag acgccgtgcc agagtctgaa gtcacgcggc
     7741 gtcaggtagt ggccgaggct cgtcagggcg atgcccgcca gcacgagggc gacggctttc
     7801 caattccacg ccgtcctctc cgcgtcgatg ctcagggtga cttcatccgg cgaagacgca
     7861 tcaccgtgag agcgagaatg gggcgagggc gactttagcc gtgcctgggg ttgcgtcgtc
     7921 atatacgctc gatctatctt ctactgccga gccttcacca agaaactgcg ctgccatcac
     7981 cgccggtacg tacgcctggg tttcccgcgg cagcattccg gatgccgaca ggcgccagaa
     8041 atcacgatct ccggcccgct cgatggcgct ctggacacga ttctcgcccg cattgtacgc
     8101 ggccaaggcc agcgcccagt ctccaaacct agcatagaga gacttcaggt agcgggccgc
     8161 ggcgcgtgtg gatttctcag ggtcggtgcg ctcatctcgc cgggccacag tcaagccgaa
     8221 tgttgtggcc gtctcgggga tgagttgcca gattccgagt gcattcttgg gggatctggc
     8281 tctcggatca tatccgctct caacgagtcc gacccacagt agttccggcg ggagcccttc
     8341 ttcccttagg acgcgcgtga tcatcggatg gtacgcccgc agccgcttta cggacgcccg
     8401 atagtgccca agtccggggc cctgaaagta gcggacgaag tccccgacga tgggccggag
     8461 agcccgctca ccggatttca cggatggatt cggattccgt gcggattggg cccgtgggga
     8521 gcccgcgtcc ttgcttgttc gctcctgctc ggcccgttcg aggacgtcgt tcagcaccag
     8581 ctcccagtga gggatgccct ccactgcaaa cagtcgcgct gtggggaccg tcgcgaggag
     8641 caacagcaac ggagctagac gagcgatcac ccttctcctt gtgttcattc gcgcttcaac
     8701 gcgatcggag gagcacttcc actgccacac gcgccccaca gcacgaaacg ccatcgtttc
     8761 aatcgattcc acgcagctct cgatcagcaa ggatctgccc tggcagcggc tggctgccga
     8821 atattgtcca accaaagtcc gagaatctcc aatgcgggtc cgcgtttcgc gttcgggacc
     8881 gccgggtctc ggccgataat acttgtgaaa tgaacgggtt acctcgatgc tccgttgagt
     8941 ggcaccgggc gtgccatacc tcgggcagtg gcaaccagga aggacaacac ggtgcgatca
     9001 cctttaatgg gacgggcgct tttgctcgtg ccgttcttcg tctccgggct gtgggcgcag
     9061 gcggatcggg cggaactgaa ggaccttctg gccgaagcat tgagtagcaa cccggaaatc
     9121 atcgctgcgc agaagcgcta cgaggcatcc cggcagaggc ccacccaagc cagcagcctg
     9181 cccgacccaa tgttctcgcc aagctacaac agcaacggca ggccttggcc cggcgcgggg
     9241 ttgggaatgg agccgacaag ccagattggg ttcatggttt cccaggaaat cccgtttcct
     9301 ggaaaacgga agctggccgg agatatggcc gtgaaggagt ccgaggcgga gtggcagcag
     9361 tatcagcaga tccaactgag tgtcgtttcg cggctgaagc aggcctacta ccgccgcgcc
     9421 tacgccttcg ctgccgtgga tgtgctggat cggaatctcg aactcctgcg aaagttcctc
     9481 cgtatcacgg aagcccgcta cgcggtcggc aaagccgccc agcaggatgt cttcaaggcg
     9541 cagacccaga tatcgattct ggaaaccaag cgggttcagt tggaacggga gaagcgcgct
     9601 cgcgaggccg agattgccag tctcgtgaac cggcccccag ggtcgcagtt gccgagaccc
     9661 gccgatctcc gccccatccc cgtgccggtg aagctcgaag agctctatgc agcggcgcga
     9721 gagaactccc ccatgctgcg ccgggacgag aagatgattc agcgcgcgga agtagcggtg
     9781 aacatggcac gcaaggaata ctaccccgac ttcacgctga acggcggtta ctacaacatg
     9841 ggccggatgc cggacatgta catgtttcgc gcggacttca agatcccact gtacttcttc
     9901 cgcaaacagc gcgcggccgt cacggaacaa gcgcaaacgc tggtggaatc gcgcaagacg
     9961 tatgaggcga caaaccagag cctgcatttc cgcattcagg acgactacct gatgacgcag
    10021 acctccgagc aactcgtccg gctctacggc caaacagtaa tccctcaagc cagcctcgcg
    10081 ctggagtctt cactgtcttc ctacgagacc gggtcggtcg acttcttgac ggtcctcatg
    10141 aaccacatca cggtcgtcga atatgaaatg aactattacg aagagctgca gaatttctac
    10201 ctggcgctgg cgcggcttga ggagatgact gcgcgaccgc tcacttaagc agtagtaacg
    10261 gataccgaaa tgaaacccgc acgaattatt ctttttgtcg ctcttgtcgc cgttgggatc
    10321 ctgatcggct ttggctacgg ccgctggtac ggacctcgcc aggccgccgg cgtcacggcg
    10381 caggtcaaga agccaacggg gtatcactgc ccgatgcatc cgagctatcg ttccgacaag
    10441 cccggcgatt gcccgatctg cggcatgaag ctggttcccg acgacgaacc ggagcatccc
    10501 ggccacgcgc cggctcaggc ctcggcggag cggaagattc tctactatcg agacccaaag
    10561 aaccccgatt tcaagtccga caagccaggt ttaaaccccg agaccggaaa cgaactcgag
    10621 cccgtgtacg cggacgagcc cgcttcgatg cctatgggaa cgatccgtgt gagtccgcag
    10681 aaacagcagt tgatcggagt gaagtacggt gaggcgacgt cggccgccgg cctgcacacg
    10741 ttccgggccg ttggaaaggt ggcgatggat gaaacccgcg tcgccaaagt gcagaccaag
    10801 atcgaaggct ggattgaaaa agtctacgtc gatttcactg gaaaggtagt cgagaagggc
    10861 gaaccgcttc tcaccctcta cagcccggag atgctggcca gccagcagga gtatctcttg
    10921 gcgatccgaa gccgggaggt gatgcagagc agtccgctcg ccggcactca gcagcaaagc
    10981 gagtcgctgc tcgatgcgtc ccggaagcgg ctggaactct gggatttgag cgacgcgcaa
    11041 atcgaagaga ttacgcgtac cggaaaacct ctcaagaaca tcacgctgta ttcgccgatc
    11101 agcggatacg taatgatgcg caatgcgttt ccgaagcagc gcatcacgcc tgagaccgaa
    11161 ctctacaccg ttgccgacct cagcaaggtc tggatcatgg cggacgtctt cgaaaacgag
    11221 acgccgatga ttcgggtagg catgcccgta cgggtctccc tgtcctacgc cggcgggcgc
    11281 aagctgcaag gacgcgtgga ctatatccaa ccccaggtgg atatgacgac tcgcacctta
    11341 aaggtgcgca tcgaggccga aaaccccgcg atggtcctaa agcccgagat gttcgtggac
    11401 gtggaattcc aggtcgccat gcccgcgagc gtcacggtgc catcggaggc agtcctcaac
    11461 accggtttga ggcagactgt tttcgttgac cgcggcaatg ggtatctgga gccgcgccag
    11521 gtggagaccg gcgagcggat cggcaaccgc atcgaaatca ccaagggact tcaggcaggc
    11581 gagagagtgg tgatctcggg caatttcctg atcgactcgg agagccagtt gaagtcagcc
    11641 gcggcaggga tggccgggca tcagcatggc ggcgcgaccc cgaagccggc cgaactgaag
    11701 ccttctactc ccgtgggaga gcaccaacat gattgaccag atcattgagt tctccggccg
    11761 caacaagttt ctcgtctttg tcctgatcgg ctttgctgtc gtcgcgggtc tctggtcgat
    11821 gacgcaggtg ccgcttgacg cgattcccga cttgagcgac acacaggtga tcgtttactc
    11881 gcgctgggac cgcagcccgg acatcatgga ggaccaggtc acgtacccca tcgtcaccac
    11941 catgctgggt gcgccgaagg tgaaggacgt ccggggattt tccgacttcg gctactcatt
    12001 cgtctatatc atctttgaag aagggaccga tatttactgg gcccgctccc ggaccctcga
    12061 gtacttgtct ggtgtcctcc ccaggctccc gcagggcgtc aagacagagc tgggcccgga
    12121 tgcgagcggg gtcggctggg tgtttcagta cgctctggtg gacgagagcg ggaaacactc
    12181 gctggccgag ttgcgctcgc tccaggactg gtttctccgc taccagttga agtccgttcc
    12241 tggagtcgcg gaagtggccc ctctcggcgg gtttgtccgg cagtaccagg tcaacgtcga
    12301 tcccaaccgg cttcaggcct acaacgtccc gatcatgaag gtcgtcgagg ccgtgcgtaa
    12361 cggaaacaat gacgtcggcg gccgcctcct ggagttcagc ggaaccgagt acatggttcg
    12421 cggcaggggc tacgcgaaaa gtaccgatga cattggaaac atcgttcttc tgagcacgga
    12481 atcaggagtc ccagtccgag taaaggacgt gggcgatgtg gttcttggcc ccgacattcg
    12541 ccggggaatc gcggactgga acgggcaagg cgacgttgtc tccggcatcg tcgtgatgcg
    12601 ccagggagag aatgcgctgc aggtgattga gcgggtgaaa gcgaagatca aggacctgga
    12661 acccggcctg ccggaaggag tgaagattgt ctcggcgtac gaccgttccg agctgatcct
    12721 gcgctcgatg gagaacctga agtccacgct gaccgaggag atggtggtgg tggccgttat
    12781 catcttcatc ttcctgtggc acatcccgag ctcgatcatc ccggtattca cgattcccat
    12841 cgccatcatc atctcgttca tcccgatgaa gctgatggga gtcagcgcga acatcatgtc
    12901 gcttggcggt attgccatcg cgatcggcgc aatggtggat gccgccattg tcgtcgttga
    12961 acagacgcac aagcaccttg aagagtggga tcgcaccgga cgccagaagg actatcaccg
    13021 ggtcgtcatc gacgcggtga aggaagtcgg cggtccgagt ttcttcgcac tgcttgtcat
    13081 tgccgtctcc ttcctcccgg tgttgacgct ggaggcccag gaaggccggc tttttaagcc
    13141 cctcgcctat acgaagaacc tgtcgatgat tatcgccgct gtcctggcga tcactctaga
    13201 tccggccatg cgcttgctgt tcactcacat ggacaacttc aacttccggc cccgatggct
    13261 ggctcgagtt acgaacgcgg ttctcgtggg gaagatccat tccgaagaga accatccgat
    13321 cagcaagatc ctgatcagga tttacgaccc cgtctgccgc tggtctctgc gctggaaata
    13381 cgccgttatc gcgggtgcgg tcgccacggt ctttgcgacg attccgatct acgaaaagat
    13441 cggctcggag ttcatgcccc ctctggacga gggcacgctc ctctacatgc cttcgacgct
    13501 ccccggcatc tcggtgaccg aagccgagag actgatgcag attcaggacc gcatcatcaa
    13561 gggcttcccg gaggttctga cggtgcttgg gaagagtgga cgcgctgaga cctccaccga
    13621 tcccgctccc ttgtcaatga tggagaccac gatcgttctc aagccacacg atcagtggcg
    13681 caaggcggat acctggtact ccgagtggac ctgggcgccc tcgtggctca cggcggtatt
    13741 ccgccacatt acgcctgatc acatcccaac cgagcaactc gtggacgaga tgaacgaggc
    13801 actgacattg ccaggtgtgt ccaatgcctg gacgatgccg atcaaggccc gcaccgatat
    13861 gctgactacc ggtgtccgga cgccggtcgg tatcaaggtg tacggcgcga atctaaagga
    13921 gatcgagcga attggtacag agattgaagg actactccca c
//
LOCUS       DATIFQ010000449         8691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_534254, whole genome shotgun sequence.
ACCESSION   DATIFQ010000449 DATIFQ010000000
VERSION     DATIFQ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534254"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1053)
                     /gene="trxB"
                     /locus_tag="VLE22_22455"
     CDS             complement(130..1053)
                     /gene="trxB"
                     /locus_tag="VLE22_22455"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HSB17230.1"
                     /translation="MQNVIIVGSGCAGNTAAVYAARANLKPLVVSGYEPGGQLSLTTL
                     VENFPGFPEGINGPELVENIKKQAESFGAEYMNGTVIEADLSKRPCRLNVDGDWQETR
                     ALIIASGASARWLGLPNEQKLIGHGVSSCATCDGFFYRGKKIMVIGGGDSAMEEANFL
                     TRFGSEVTVVHRRDEFRASKIMLERAHANPKIKWLTNTVVDDVFDVEKGVVTGVRLRS
                     TKDGHLWEQEVDGFFVAIGHVPNTKVFAGQIELDPDGYIVSKGGARTSVTGVFHAGDV
                     QDRVYRQAITAAGAGCMAALEAERLLEAEGH"
     gene            complement(1119..3026)
                     /locus_tag="VLE22_22460"
     CDS             complement(1119..3026)
                     /locus_tag="VLE22_22460"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HSB17231.1"
                     /translation="MSDCHGCGSAPTTTLAVPTHKKGAGPRTVAIVGPPNSGKSTLFN
                     RLTGLRQKVANFPGVTVEQRIGQARLGANKEVTVVDLPGVYSLSPRSEDEQVTFDVLQ
                     GCMEGVSKPDAILLILDATNLGRHLVLAAPVLALGIPTLVILNMADDLRARGGKLDVE
                     ALARQLGAPVTLISASRGEGVDTVFKFLGGTVGVPAPVELPVIHDVPHCRQWAGRLGD
                     RAAYHPPAPPRWTRRLDAIFLHPLKGPIVFGLVVVAVFQSIFSWARPLMDGIQSLVLM
                     SGAWLENLLPESALRSLLIEGVWGGVGSVVVFLPQILLLFLFIGILEDSGYLARAALI
                     ADRTMARVGLQGKSFIPLLSAYACAVPAILATRTIENKRDRIATILIAPFMTCSARLP
                     VYTLIIAAFLPERRLLGPFLGTRAAALLGLYLLGFLAAVTTARLLKSSILKSERTPFV
                     LEMPPYRWPTLQSLGLRLLDRSKIFLRRAGTVILLMAVILWIMAHLPLQSGRAPAIAD
                     SVAGTIGRTVEPLIRPLGFNWKIGIGLITSLAAREVIVGTLGTIYGMEGDAQSIGLQA
                     ALRQDLHLGGAVALLVFFAFAMQCMSTIAVVRRETGGWKWPVLQFCYMGGLAYAGALV
                     ANQLVRLWTGA"
     gene            complement(3036..3305)
                     /locus_tag="VLE22_22465"
     CDS             complement(3036..3305)
                     /locus_tag="VLE22_22465"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HSB17232.1"
                     /translation="MVQLSFNRSPRSLTDLREGEHGVLDHLELPEDMSRRLMELGFLP
                     GSRVTAAGSAPGGDPRVYRVDGSEVALRRETATHLMLRKPPKFRP"
     gene            3391..4344
                     /locus_tag="VLE22_22470"
     CDS             3391..4344
                     /locus_tag="VLE22_22470"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17233.1"
                     /translation="MLGSEPKQGYSRIEVRRMLNFTERQLRSWEKDRLIPRLETFAFS
                     DLIALRTLVQLKRAGISTPKIRRAVEALRDKLSAVSDPLRDLKIFSDGRKITVQVEGG
                     KMEPVSGQLLLDFDRESLHKMLSFPRHGDRDDDKAARQARQLEATLWFEKALELEQTG
                     SPVVEIIGAYEKAVALDPLSAGAFVNLGTIYFHQRKWDEAERNYRKALEADSSYSLAH
                     FNLGNLYDEKGDRSKAFLHYLMAIRLNPSYGDAHYNLALLYQSSGQALRAVRHWKAYL
                     KIDPASPWAAIARQELDKLCRDTIVTGSKSRTRGPDAESVS"
     gene            4414..4935
                     /locus_tag="VLE22_22475"
     CDS             4414..4935
                     /locus_tag="VLE22_22475"
                     /inference="COORDINATES: protein motif:HMM:NF022273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HSB17234.1"
                     /translation="MTRLLQAIRSFFSILFSGRLPADVAAALGLAKAAPKPGTRPPEP
                     PVRPVDGALQLLAILQRDARLVDFLLEDVSGYSDEQVGSAVRSLHEQCRQVLTRYLRL
                     SPVIDGVEGTYTKVESPDPARVKFIGNLPTKGTPAGGTLRHKGWRAEKIDLPPVDRRH
                     DLSVIAPAEIEVE"
     gene            4942..6765
                     /locus_tag="VLE22_22480"
     CDS             4942..6765
                     /locus_tag="VLE22_22480"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSB17235.1"
                     /translation="MQPTYVIGIDLGTTNCGLAFAEARVESPHQLPTVLPFAVPQLVN
                     PGEVREEALLPSALFLPGPTDFPAGSIALPWDPEPAAVTGQLAQKRGVENTRRLVASA
                     KSWLCHAGVDRTSPILPVGAPDGAPKISPVEASRRYLEHLRNAWNWARPDASFDAQNI
                     LVTVPASFDAVARDLTLRAAGDAGYANVVLLEEPQAAFYAWIERHPDWRDRVRVGDLI
                     LVVDIGGGTTDFTLIAVTERDGELQLERVAVGEHILLGGDNMDLALARFIEQQLSAGG
                     RKIDSFQLHALWQNCRLAKERLLEDGNTAEEHPVTILGKGSGLVGGTIKAALRRGDIE
                     RILLDGFFPQTSSNDMPQRRRAGLQELGLPYAADAAITRHLARFLRQQASSAEHGSVR
                     RGPSGLACPTHVLFNGGVLRAGLVRERLLSTLNVWLDGESMPAARPLEGDDLMHAVSR
                     GAAYYGLARLGHGVRIRGGVSRTYYVGIETAMPAVPGLPAPLKALTVAPFGMEEGTEL
                     RLTGREFGLVVGEPAEFRFLTSASRKDDPAGHLLDEVGDDVEELSPMEVTLPSAGDAG
                     EVVPVTLETVVTETGLLQLWCVARDGRRWKLEFNVRERVSP"
     gene            6762..>8691
                     /locus_tag="VLE22_22485"
     CDS             6762..>8691
                     /locus_tag="VLE22_22485"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSB17236.1"
                     /translation="MKVGIDLGTTNSALAYIDPAEAAGADFPPVHLLPIPQHVEQAIV
                     EPRRTLPSFLFLGDDRQYVGTFAREQGALVPARVVHSAKSWLSNPDVDRTAKILPWDA
                     RESGRILSPVEVSARFLGHIRESWDAARSEPLSAQEIVLTVPASFDEEARELTVAAAA
                     EAGLANFTLLEEPAAAFYSWIANHLSQSRKDLFDGQLVLVCDVGGGTSDFTLIRIHRE
                     GDRIDFTRTAVGRHLLLGGDNLDLTLAWLVETKLGVALSIRQRSALRRQCAAAKERLL
                     SDASLPSVEIRVLGAGTSIVGGTLKTEILREEALELALEGFLPFCQLSDRPQEEKRSL
                     FRELGLPYVSDPAVTRHLAAFLASAGLDAAGPDAILFNGGFFMPDLLRERVADVVGTW
                     YGRRPLVLENRDLDLAVAVGAAYYSYVRSTGSGLLVRGGLPRTYYIGIGGGPGGEKAR
                     AVCLVPRGAEDGAFLELDRDDLQLVANRPVSFRLYSSLTRTGEMLGDVVDFDAAQLGD
                     EVHVHAPLHAVIRFGKRTGDRLVPVKLAARLTEIGTLEIWCESKVSEHRWRLQFELRK
                     TAVETEKPRAAAVVSEEAVAGAAEIVRTAFSTSERPLAPEELPARLEQAMGLGRNSWP
                     LGVIRRLADVFLETAEGRR"
BASE COUNT         1519 a         3038 c         2564 g         1570 t
ORIGIN      
        1 tgtcttgcgg ctcccgttcc gacggccgcg cctgccgcga tcagaccaag aaaatcccta
       61 cgtctaacac tctccatgag tttccctgcc gccgtttcca gtcggtccac cggggtacgg
      121 gggatcgcct tagtgacctt ccgcctccag gagccgttcc gcctccaggg ccgccatgca
      181 tcccgctccg gccgcggtga tcgcttgccg gtagacccgg tcctgcacgt ccccagcgtg
      241 aaagacgcct gtaacgctcg tgcgcgctcc gcctttcgag acgatgtagc cgtccggatc
      301 cagttcgatc tgtcccgcga agacttttgt gttcgggacg tggccgatcg ccacgaagaa
      361 gccgtccacc tcctgctccc acaagtggcc gtccttcgtg cttctcagac gcacgcccgt
      421 cacgacgccc ttctccacgt cgaagacgtc gtccacaact gtattcgtca gccacttgat
      481 cttcgggttg gcgtgcgcgc gttccaacat gatcttcgag gcgcgaaact cgtcacgccg
      541 gtggactacc gtgacctcgg agccgaagcg agtcaggaaa ttcgcctcct ccattgccga
      601 gtcgcctccg ccgatcacca tgatcttctt accgcggtag aagaagccgt cgcaggtggc
      661 gcatgagctt acgccatgtc cgatcagctt ctgctcattg ggcagcccga gccagcgcgc
      721 tgacgctccc gaggcgatga tcagcgcgcg cgtctcctgc cagtcgccgt caacgttcag
      781 ccggcacggt cgcttactca ggtccgcctc aatgaccgtc ccgttcatgt actcggcccc
      841 gaaactctcg gcctgcttct tgatattctc gacgagttcc gggccgttga tcccttccgg
      901 aaaaccggga aagttctcca ccagcgtggt cagcgacagt tgtccaccgg gttcgtagcc
      961 gcttaccacc aggggtttca gattggcgcg agcggcgtaa actgcggccg tgttacccgc
     1021 gcagccggat ccgacaatga ttacgttttg catgggagga atgaacctgc gttggaattt
     1081 cttatgctag catgacgccg ccggagccct gctcgatttc aggcgcctgt ccacaagcgg
     1141 accaactggt tggcgaccag cgcgcccgcg tatgccagtc cacccatgta gcagaattgc
     1201 agcaccggcc atttccatcc gcccgtctcg cggcgcacaa ccgcaatcgt cgacatgcac
     1261 tgcattgcaa acgcgaagaa gaccaggaga gccaccgcgc ccccaaggtg cagatcctgc
     1321 cgcagcgccg cttgaaggcc gatggactgc gcgtcgccct ccattccgta gatcgtgccc
     1381 agtgtgccga cgatcacctc gcgcgcggca agcgacgtga tcagtccgat accgatcttc
     1441 cagttgaagc ccagcggccg aatcagaggc tccaccgtcc ggccgattgt gccggcgacg
     1501 ctgtccgcaa tcgcgggcgc ccggccgctc tgcaacggca ggtgcgccat gatccacagg
     1561 atgactgcca tgagcagaat caccgtgccc gcccgccgca agaatatctt ggagcgatcg
     1621 agcagccgca gaccgagcga ttgcagcgtc ggccagcggt aaggcggcat ctccaaaacg
     1681 aacggcgtcc gttcgctctt caggatcgac gatttcagca gtcgcgctgt cgtcactgcc
     1741 gctagaaagc cgagcaagta caatcccagc agcgccgccg cccgcgtgcc gagaaacggc
     1801 cccagcagcc tgcgctccgg aaggaacgcg gcgatgatca gcgtgtacac cggcagccga
     1861 gccgagcagg tcatgaacgg ggcgatcagg attgtcgcga tacggtcccg cttgttctcg
     1921 atcgtgcgtg tcgccaaaat cgccggcacg gcgcatgcgt acgccgacaa cagcgggatg
     1981 aacgacttgc cctgcagccc gacccgcgcc atcgtccggt cggcgatcag cgccgcccgg
     2041 gccaggtagc cggaatcctc cagaattccg atgaacagaa acagcagcag gatctggggc
     2101 aggaagacca ccaccgagcc cacaccgccc cacacaccct cgatcaagag ggagcgcagc
     2161 gcggactccg gcaaaaggtt ctccagccat gcacccgaca tcagcaccag ggattggatc
     2221 ccgtccatca acggccgcgc ccaagaaaaa atcgactgaa agacggcaac cacaaccagc
     2281 ccgaaaacaa tcgggccttt cagcggatgg agaaagatcg catccagacg gcgtgtccag
     2341 cgcggtggtg ccggggggtg atacgccgcc cggtctccaa ggcgccctgc ccactgccgg
     2401 cagtgcggca cgtcgtgaat gacgggaagt tccaccggcg cgggtactcc cactgtgccg
     2461 cccaggaact tgaacaccgt gtcgacgccc tcgccgcggg aggcgctgat cagagttacg
     2521 ggcgcgccca gttgtcgcgc caaagcctcg acgtcgagct ttccgccgcg ggctcgcaag
     2581 tcgtccgcca tgttcaggat caccagagtt gggatcccga gagccagcac gggcgccgcc
     2641 agcaccaggt gccgcccgag gttggtcgca tcgaggatca gcagaatagc atcaggcttc
     2701 gacaccccct ccatgcaacc ctgaaggacg tcgaacgtca cctgttcgtc ttcgctccgg
     2761 ggagacagac tgtaaacccc cggcaggtcg accacagtga cctctttgtt cgcacccaag
     2821 cgcgcctgcc cgatccgctg ctcgaccgtc acgccaggaa aattggctac cttctgccgc
     2881 agcccggtga gccggttgaa cagtgtcgac ttccccgagt tcggcggtcc cacgatcgcc
     2941 accgtgcgag gtccggcgcc ctttttgtgt gtcggtaccg ccagtgtggt cgtcggcgcg
     3001 gagccgcagc cgtgacaatc gctcatttcg agctcctacg ggcggaattt tgggggtttt
     3061 cgaagcatca gatgggtagc agtctcccgc cgaagggcga cctcggagcc gtcgacgcgg
     3121 tatacccgcg gatcgccgcc cggcgcgctg ccggctgccg taacccggct tcccggcaag
     3181 aagcccagct ccatcagccg ccgcgacatg tcctccggca gttcaagatg gtcgagaacg
     3241 ccgtgttccc cttcgcggag gtccgtcagg gatcgcgggc ttctgttgaa actcagttgc
     3301 accacgattg tagtatgaga cctcgttgtc ggaaagtcaa ggagcctcca ccctacggga
     3361 aacctctctt cccatgctac aatttctcgc gtgctgggta gcgagccaaa gcagggctac
     3421 tcccgtatcg aggtccgccg catgctcaac ttcactgagc ggcagcttcg aagctgggag
     3481 aaagaccgcc tcatcccgcg tctggagaca tttgccttct ccgatctcat cgccctgcgc
     3541 acactcgtgc agctcaagcg agccggaatc tctaccccga agatccggcg cgccgtcgag
     3601 gcgctgcgag acaagctaag cgccgtctcc gaccctctcc gcgatctcaa aatcttctct
     3661 gacggaagaa aaattaccgt tcaggtcgag ggcggcaaga tggagccggt gtccggccag
     3721 ttgctcctcg atttcgaccg tgaatcgctc cacaaaatgc tctcgtttcc tcgccatggc
     3781 gatcgggatg acgacaaggc tgcacgccag gcccgccaac tcgaagccac cctctggttc
     3841 gagaaggcct tggaacttga acagaccgga tcccccgtcg tcgagatcat cggtgcgtac
     3901 gagaaggccg tcgcactcga cccgctatcg gctggcgcgt tcgtgaacct gggcaccatc
     3961 tatttccacc agcggaaatg ggacgaggcc gagcgcaatt accgcaaggc tctcgaggcc
     4021 gactcgagct actcgctcgc ccactttaat ctcggcaacc tgtacgacga aaagggggat
     4081 cgctccaagg ccttcctgca ctacctgatg gcgatccgcc tcaacccgtc ctacggcgac
     4141 gcacactaca acctcgccct tctctaccag agctccggcc aggctctccg cgcagtccgc
     4201 cactggaaag cctacctgaa aatcgacccc gctagtccct gggcggccat cgcccggcag
     4261 gaactcgaca agctctgccg cgacacaatc gtgaccggct ccaagtcgcg cacccggggt
     4321 cccgacgccg aaagtgtatc ctagaagttc gaccatcgaa tcccagccgc gaccaaaggg
     4381 agcggtccga caaagcagtt tcggagacaa accttgaccc gcttgttgca agccattcgc
     4441 agtttctttt cgattctctt ctccggccgg ctgcccgccg atgtcgccgc tgcgctcggc
     4501 ctggcgaaag cggctcctaa gcccggaacc cgtccgccgg agccacccgt gcgtccggtt
     4561 gatggagcgc tccagttgtt ggccatcctc cagcgggacg cccgcctcgt cgacttcctc
     4621 ctggaagacg tctccggcta ttccgacgag caggtcggct ccgccgtccg tagcctccac
     4681 gagcagtgcc gccaggtgct tacccggtat ctacgcctgt cgccggtcat cgacggcgtg
     4741 gaaggcacgt ataccaaggt ggaatcgccc gaccccgccc gggtgaagtt cattggcaac
     4801 cttcccacca agggaacccc tgccggcgga acgctccgtc acaaggggtg gcgcgcggag
     4861 aagatcgatc tgccgccggt cgaccgacgg cacgacctca gcgtcatcgc cccggcggaa
     4921 atcgaagtcg agtagcctgc attgcagcca acatacgtca tcggcatcga cctcggcacc
     4981 acgaactgtg gccttgcatt cgccgaagca cgcgtggagt cgccgcatca gttgccgact
     5041 gtgcttccgt tcgcggttcc acaactggtg aacccgggcg aggttcgcga ggaagctctc
     5101 ctcccctcgg cgctgttcct tcccggtccc accgacttcc ccgccggctc catcgcgctt
     5161 ccctgggatc cggaaccggc ggccgtcacg ggccaactcg cgcaaaagcg cggtgtcgag
     5221 aatacccgcc gtctcgttgc gtctgccaaa tcatggctct gccatgcggg ggtcgaccgc
     5281 acttcaccca tcctgcctgt tggcgcgccg gacggcgcgc cgaagatctc tccggttgag
     5341 gcgtcgcggc gatatctgga gcacttgcgc aacgcctgga attgggcgcg cccggatgcg
     5401 tcattcgatg cgcagaatat cctcgtgacc gttcccgcgt cgttcgacgc tgtcgctcgc
     5461 gaccttaccc tgcgcgccgc gggagacgcc ggctacgcga atgtcgttct gctcgaggag
     5521 ccgcaggcgg ctttctacgc ctggattgag cgccacccgg actggcgcga tcgggtgcgc
     5581 gtcggcgatc tcatcctcgt cgtcgacatc ggcggcggca ccaccgactt tacgctcatc
     5641 gccgtcactg agcgcgacgg cgagcttcaa ctggaacgcg tcgccgtagg cgaacacatc
     5701 ctgcttggcg gcgacaatat ggacctcgcg ctcgcgcgct tcatcgagca gcagctctcc
     5761 gcgggagggc ggaagatcga ttccttccaa ctccacgcgc tttggcaaaa ttgccggctc
     5821 gccaaggagc gtctgctcga agacgggaac acggccgaag aacacccggt gaccattctc
     5881 ggtaaaggct cggggctggt tggcggaacc atcaaggcgg ctctccgccg gggagacata
     5941 gaacgcatcc tgctcgacgg tttcttcccg caaacatcga gcaacgatat gccgcagcgc
     6001 cgccgcgccg gtttgcagga actcggcctg ccgtacgccg ccgacgccgc gattacgcgg
     6061 cacctcgcgc gctttctccg ccagcaggcc tcatccgccg agcacggctc agtacgtcgc
     6121 ggcccgagcg gcctcgcctg tccgacgcac gttctcttca acggcggtgt gctgcgcgcc
     6181 gggcttgtcc gggaacgtct gctctccacg ctgaacgtct ggcttgacgg cgaaagcatg
     6241 ccggctgcca ggcccctcga gggggacgac ctcatgcacg ccgtctcccg cggcgcggcc
     6301 tactatggtc ttgcgaggct cggccacgga gttcgcatcc gcggcggcgt ctcgcgcaca
     6361 tactacgtcg gcatcgagac cgccatgccc gctgtgcccg gccttccggc tccgctcaaa
     6421 gcgctcaccg tcgctccgtt cggtatggag gagggaacag agctgcgtct aaccgggcgt
     6481 gaattcggtc ttgtcgtcgg tgaaccggcc gagttccgtt tcctcacctc tgcgtcgcgc
     6541 aaggacgacc ccgctggtca tctcctcgat gaggtgggcg acgacgtcga agaactttct
     6601 cccatggaag tcaccttgcc ttcggccggc gacgccggag aagtcgtgcc ggttaccctg
     6661 gagaccgtcg ttactgaaac cggcctcctc cagttatggt gcgtggcccg cgacggtcgc
     6721 cgctggaagc tcgaattcaa cgttcgcgaa cgggtttctc catgaaagtc ggcatcgatc
     6781 tcggcactac gaactccgcc ctcgcataca tcgatccagc cgaagctgcc ggcgccgatt
     6841 tcccgccggt gcatttgctc cccatcccgc agcatgtcga gcaggcgatc gtcgaaccgc
     6901 ggcgcacgct gccgtcattc ctctttcttg gagacgaccg gcagtatgtc ggtacgtttg
     6961 cgcgcgagca aggtgcgctc gtgccggccc gtgtcgtgca ctccgcaaaa tcctggctct
     7021 cgaacccgga tgtcgaccgc accgcgaaga tccttccctg ggacgcccgc gaaagcggcc
     7081 ggatcctttc gcccgtcgag gtctccgcgc gcttccttgg ccacatccgc gaatcatggg
     7141 acgccgcgcg gtccgagcct ctctcggccc aggaaatcgt tcttacggtg cctgcttcgt
     7201 tcgacgaaga ggcgcgggag ctgacggtcg ccgccgccgc agaggccggt ctcgccaatt
     7261 tcaccctgct cgaagagcca gccgcggcgt tctattcgtg gatcgccaac cacctgtcgc
     7321 agtcccggaa ggaccttttt gacggccagc tcgtgctggt ctgcgatgtc ggcggcggga
     7381 cgtcggactt cacgctcatc cgcattcacc gcgaaggcga tcgcatcgac ttcacccgca
     7441 ccgccgtcgg ccgtcatctc ctgctgggcg gcgacaacct cgacctcacc ctcgcctggc
     7501 tggtcgaaac caagctcggc gtcgcgctct ccatccgtca gcgcagcgct ctccggcgcc
     7561 aatgcgccgc cgccaaggaa cgcctgcttt ccgatgcctc tttgccctcg gtcgagatcc
     7621 gggtgcttgg cgctggcacc tccatcgttg gcgggactct caagaccgag atcctgaggg
     7681 aggaggcgtt ggagctcgcc ctcgaaggct ttctgccctt ctgccaattg tccgaccggc
     7741 ctcaggagga gaagcgcagc ctcttccgcg agctcggcct gccgtacgtc tcagaccccg
     7801 ctgtcacccg tcacctcgcc gctttcctcg cgagcgccgg cctcgacgcc gccggtcccg
     7861 acgcgatcct gttcaacggc ggcttcttca tgcccgatct gttgcgcgag cgcgtggccg
     7921 atgtggtcgg aacttggtac gggcgcaggc cgctcgttct cgagaaccgc gatctcgacc
     7981 tcgccgtcgc cgtcggagcc gcctattact cctacgtccg ttctacaggc agtgggctcc
     8041 tcgttcgcgg tggcctgcct cgcacatact acatcggcat cggcggcggt ccgggcgggg
     8101 agaaggcgcg ggccgtctgt cttgtgccgc gcggggccga agacggcgcc ttcctcgaac
     8161 tcgatcgtga cgaccttcag ctcgtcgcca accgccctgt ttctttccgt ctctacagct
     8221 cgctgacgcg taccggcgag atgctcggcg acgtcgtcga cttcgatgcc gcgcaactcg
     8281 gcgatgaagt acacgttcac gcaccgctcc acgccgtcat ccgcttcgga aagcgaaccg
     8341 gtgaccggct cgtgccggtg aaactggccg cccgcctcac cgagatcggc accttggaaa
     8401 tctggtgcga gtcgaaagtt agcgagcacc gctggcggct ccagttcgag ctgcgcaaga
     8461 cagccgtcga gacggagaag ccgcgagccg cggcggttgt cagcgaggag gctgtcgcgg
     8521 gtgccgccga gatcgttcgc accgcgtttt ccacgtccga acggccgctc gcgcccgagg
     8581 agcttcccgc ccggcttgaa caggcgatgg gactcgggcg gaattcgtgg ccgctcggcg
     8641 tcatccgccg tcttgccgat gtcttcctcg aaacggccga ggggcgccgc c
//
LOCUS       DATIFQ010000450        18784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2643814, whole genome shotgun sequence.
ACCESSION   DATIFQ010000450 DATIFQ010000000
VERSION     DATIFQ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 18784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18784
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2643814"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VLE22_22490"
     CDS             <1..1170
                     /locus_tag="VLE22_22490"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB17237.1"
                     /translation="KIDRPQIDCHIAYTTDETHRILRDNISRSPLYSGRIEGVGPRYC
                     PSIEDKIIKFPEKIRHQIFLEPEGLDTYEVYVNGMSTSMPIDVQTAMVASVPGLEDAE
                     MIRPGYAIEYDAIDPRELDHTLEVKSIRGLYLAGQINGTSGYEEAACQGIIAGLNAAC
                     RLGGMEPLVIGRNEGYTGILIDDLITKGAEEPYRMFTSRAEFRLHLRIDNADERLTPA
                     GRRVGLVTEERWRLYTRKQEQKQRLLPLLEQTRVSPDILPDVNLPSGDRPTLAQWLKR
                     PEARIGALTGWIAGILGEPPVRGVLTTLETEIKYDGYMAQQERQIERLKNAEGRRIPD
                     TFTYHNVPGLSLEVRDKLQRVRPATLGQAGRIPGVTPAAIAVLDVYLTLARENAS"
     gene            1204..1809
                     /gene="rsmG"
                     /locus_tag="VLE22_22495"
     CDS             1204..1809
                     /gene="rsmG"
                     /locus_tag="VLE22_22495"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HSB17238.1"
                     /translation="MTRFRTLLDENLSGICTLTNAQIEQLYGHFKLLSHWNERINLTS
                     IRDVDTAVLRHYCESLFFASRLPKSFVSIADVGSGAGFPGIPIAVLRPDCRVTLIESV
                     QKKALFLREATRGWENVRVVARRAEEVEEQFDLIVARAVSPKGVVRLVPRLAPAVGLS
                     IGEDNLHKLLKIKDIIWDHPEKLPWGERRVILIGRQVSRGT"
     gene            1915..2709
                     /locus_tag="VLE22_22500"
     CDS             1915..2709
                     /locus_tag="VLE22_22500"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HSB17239.1"
                     /translation="MGKVIAIANQKGGVGKTTTAINLAASLAANDLRVLLVDADPQGN
                     STTGLGVSKDHERASLYHALMGQREMGSITVQTDFFGLDLIPADRNLVGANLELIEFE
                     GRETRLRQTLQTLRDKYEYILIDCPPALDLLTLNALVAADSVLIPIQCEFFALEGISQ
                     LLDTVERIRESFHHPLKIEGVLLTMFDDRTNLTRQVAADLREFFSDEVFKTVIPRSVR
                     LAEAPSYGKPILAYDIRSRGAESYIRLAKEILANDQDVRTPTEGAR"
     gene            2669..3571
                     /locus_tag="VLE22_22505"
     CDS             2669..3571
                     /locus_tag="VLE22_22505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HSB17240.1"
                     /translation="MTKTSEHPRKALGKGLSALLAPRAPQLASAPAPVPRRPEGEEVL
                     RIPVTQIDPNPLQSRTIFQAEKLDELAQSIKANGVIQPLIVRRHENRYQLVAGERRWR
                     ASKIAGLTEVPVVVQNLTDEQLLEVTLIENIQREDLTPIEVAHAFERLVRELGLSHDE
                     IGRRTGKDRSTITNLLRLLRLPPDIQQLLTEHRLSMGHARAILGLPSEELQRQVAEKA
                     SAQGLSVRQVERLIQRMTESRETKSPDEVSIDPNVKAAIDELERVLGTRVRIQQKSDH
                     RGRIEIEYYSTDDLDRIYTLIVGD"
     gene            complement(3583..3658)
                     /locus_tag="VLE22_22510"
     tRNA            complement(3583..3658)
                     /locus_tag="VLE22_22510"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3623..3625),aa:Met,seq:cat)
     gene            4178..5491
                     /locus_tag="VLE22_22515"
     CDS             4178..5491
                     /locus_tag="VLE22_22515"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB17241.1"
                     /translation="MTSVEFQGVSKSYPIYASPGDRLKELLTLNRRSFHRDFWALRDV
                     SFEVRRGETFCVIGENGAGKSTLLQMVAGILPPASGTVRVEGRVSALLELGSGFNPEF
                     TGRDNVYLNAAILGFSRQQIDRKYREIQEFAEIGDFVDQPVKTYSSGMVVRLAFAVAI
                     HVDPEILLVDEALAVGDIYFRQRCMRKVHELRSRGITILFVSHAIADVKAIGDRTLWL
                     EEGQVKDLGTTDAVVARYLAAMAEKDSAYLNLTHRPAPESSRAQYRAPEIVETIPNID
                     HRHGDGRAEVIGIAVLDSTGHPLPLLEPQSRVVVRISVRAKEEISLPVVGFMLRNHLG
                     IDFAGFNTAREGIELPPMRPGDIHTVDFHVDFPELYPGTFSFSPAIADGPLVSYRTCD
                     WIDNAVTLQMGHSGGQVYGYMRIPCRVTLNSRLHSEARETETKVG"
     gene            5484..6659
                     /locus_tag="VLE22_22520"
     CDS             5484..6659
                     /locus_tag="VLE22_22520"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB17242.1"
                     /translation="MAEFTGERVIPGQVDGDLWNEHIARYAFASRLARLKRVLDAGCG
                     SGYGAADLARCAATVVGVDNSAEAVAYARAHYPLPNLRFLEGSCSSLPLATGSFDLVV
                     SFEVIEHLQDWATFLSEVRRVLAPGGQAIISTPNSQYYTDSRGESGPNPWHEYEFAFE
                     EFRDELLKVFPHVSMFVQNHVEGFVFQPVKTFSTAEARVESGGGGPEDSHFFVAVCAL
                     SPQTGAPTFIYLPKAANILREREQHIVKLQKEMAIKDQMLLDMEKELKERSEWAERLN
                     SELREAGATIERLHRELGEMSRGYEAKVGELEEENRKRAEWALETERRLEDKVQELAK
                     CVDVLHETEKTLDERTTWAFGLQKQVRDVEYQLALVRGSRWMKLGNAFGIGPPLPKE"
     gene            6661..9018
                     /locus_tag="VLE22_22525"
     CDS             6661..9018
                     /locus_tag="VLE22_22525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB17243.1"
                     /translation="MTVFPRVLRALPLLLISPLLILLTAAALVAADLVWFLLARRWLL
                     PETGPAKQATSVVIPNWNGRDLLEEYLPSVVAALEANPENEILVVDNASEDGSAACLR
                     ERFPHVRVLEMPRNLGFGGACNAGVMAAANDIVVLLNNDMRVEPDFLRPLLEGFADEK
                     VFSVSCQIFFTNPEKAREETGLTQGWWQSGALRVRHRIDEGVKTLYPCFYGGGGSMAV
                     DRRKFLELGGFDRLLEPFYLEDTDLGYMAWKRGWKVMYEPRSVVYHEHRGTIGRRFSA
                     REIELVLEKNFILWSWKNIHEWPRLASHFFYCYAGALASVVFGKSPERATLEGLWRAC
                     RQLPQAVRSRWNARDLAAVNDTEAFRRPLGGYFRDRFATVPTELDRLRVLFVSPYPIV
                     PPVHGGGVFMYQTLQELVRHCEVHLVVLLHYPHELAAHDCLRGLCASAEFLVKKTVTG
                     NGAGSILPHAVREFANSDLEWLIHRQIHTQEIDVVQLEYTPMGQYGGDYRRVVNVLFE
                     HDIYFQSIGRGWKQAFGAGGKLKAAVEYLRALRYETSILERMDRIQTCSTANRDYLLS
                     FAPDLDGRIQDGLRAGIDTSRYHFRPDGREPATMLFLGNFRHLPNRTALDWFIENVLP
                     RVRKQRPDCRLVVVGSELPPRHAFSSVENIELAGYVDDIRGPLERYSVFVCPILSGSG
                     VRVKLLEAFAAGIPVVSTRLGAEGLASVDREFCALADDAETFATDILDLFDHPDRAAQ
                     MATRARSEVAERWDMGKVTAKLAASYREAVREKRFTTESTENTED"
     gene            complement(9067..11268)
                     /locus_tag="VLE22_22530"
     CDS             complement(9067..11268)
                     /locus_tag="VLE22_22530"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSB17244.1"
                     /translation="MDANRVFARMVPLLLLLNAAPATVEAQSAVDFSPEWNVLYGRAE
                     FEQADRMLDAYLRKLTAAVEAEHEGAIATVKNTADLERFQTAARERLRAAVGALPGRS
                     PLNSHVTGTLDRGDYFVEKLLFESRPRFYVTANVYVPRRVKAPFPAVLANVGHWGAGK
                     AYPDYQRLAMYLARRGFLVLVYDLPGMGERVESWDPVFRRPIIHPGTSEYFVTTEHGL
                     AAGRTILTSGNLVSYLLWDALRGLDYLESRNDVDGKRIAVTGISGGGWLTELLTAYDP
                     RVRVAIPVCYGGCIADNLFRASMTVADVDALIAPRPLLMMEATGDSRSSVAEKRRRHE
                     LVARLYEVAGAAERTQFRIFDGPHGFTDAMFPVAYSWLTRWLPGIAADSVEEPRTALE
                     TEADLAATLTGQVRTSIGGETLSSLNRARFRGLRAGAAAPAGREQWQRWQARLRRTIS
                     GKIALPSSAEPLQANTLVRADKGAYTLEKLVYYSEPGIYVPALLLLPRSVKPAPGIIF
                     VNDNGKSAGGVVERYLRPLVEAGRAVLAIDVRGAGETGQELVKDYADLGTGEEAGRFY
                     SALRVGRNVLGMRAYDVLRAVDYLEARKEVQRGAVAAVGSGSGGLLVLYAAALDDRIR
                     SAAVTGALLSYASIVEGEIFSHPLSDFVQDALQEFDLPDLAALVAPRPLLLLNPVDEA
                     QRLAGEKLATGVYRRSAGVYAMLGAGGNFTVRYADSPAGILESWKDVTSAK"
     gene            complement(11312..11869)
                     /locus_tag="VLE22_22535"
     CDS             complement(11312..11869)
                     /locus_tag="VLE22_22535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17245.1"
                     /translation="MKPVVLLLCCIALMASLPIAGVQTPKVQSVYLFPMTGGLDQFLA
                     NRLTSEGVVQVVTDPKKADAVLTDQIGEVFEKRLADLYPAETPAETKPAAAAPEKAET
                     SEKKETATSGDSEATEEARKTRERPVRLFSSGRARGTLFLVDAHTRNVIWSAFEKPKN
                     TTAEQMDRTARRIAERLKKDLTGGK"
     gene            11890..12576
                     /locus_tag="VLE22_22540"
     CDS             11890..12576
                     /locus_tag="VLE22_22540"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB17246.1"
                     /translation="MYKDLTVTVIIPCLNEEQGVEQVLKSMPAFVDEVIVVDNGSTDR
                     TSDVARFHGAKVIREEVRGYGRAYKRGFAHATGDLIVTLDGDHSYPVDALSYLLEAFL
                     HLEVDFLNTSRFPVRDPRAMSLKHWVGNIILSLVMSALFFRWVRDSQSGMWVLRRSIL
                     NDMHLESDGMAFSEEIKVEAIRNPRIRFREITILYSSRLGEVKLNPWRDGIHNLLFLV
                     KKRFARKRRK"
     gene            12659..13639
                     /locus_tag="VLE22_22545"
     CDS             12659..13639
                     /locus_tag="VLE22_22545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB17247.1"
                     /translation="MIVPTWNRRELLERVLQSLFRQTHPIREILVVDNGSEDGSAEAA
                     ERQGACVIRIGCNSGFSRAVNRGIGEARTEWLAIVNNDVVLEPDWLSRLIDGSTGNEV
                     WFATGKLLREGRRDSIDGTYDAICRGACAWRVGHARPDGPEFDESRPIRLAPFTAALF
                     RSELFARVGLLDESFESHLEDVDFGLRCASGGYGGVYVPRAVAVHTGSATEGAWNRET
                     VRRIARNQLLLVAKHYPSNWLVRYGWPVLVAQTLWGFVAAAHGGGTAYLRGKLEGARR
                     FRGLRRDGIRGGMVDPEKLARVLEESEQEILRLQRRTGFDRYWRLYFALT"
     gene            13636..14586
                     /locus_tag="VLE22_22550"
     CDS             13636..14586
                     /locus_tag="VLE22_22550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB17248.1"
                     /translation="MREAGAVIVTYNSGAEIGPCLDAAIPRAGEVVVVDNGSMDNTLE
                     EVRKRLSVKLIVNSQNRGFAGAVNQGFAATESPYVLLLNPDAVLLTGVEPMAAACARP
                     GIGAAGGKLLDADGRAQDGFMVRRFPTALALILEVLGVNRLWKRNPVNRRYRCLDMDP
                     DAAAEAEQPAGAFLMIRRDAWQAAGGFDEQFHPLWFEDVDFLKRLRDKGLQAAYEPAA
                     AARHEGGHSIQRLSQESRILYWYASLLRYSSKHFTPSRLAAVCVATALGSVLRMIGES
                     ALSRNLRPLRVYSKVVRLACVRLMLCRFAGTGSSPVLARH"
     gene            14633..15682
                     /locus_tag="VLE22_22555"
     CDS             14633..15682
                     /locus_tag="VLE22_22555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB17249.1"
                     /translation="MAHNDLVSVTIVTYNSGRFIRRCLESVLAQKYPNKEIIVIDNAS
                     TDGTIDILEQFEDRCQIVYNEENLGFAAAQNQAIDLSTGEWVMTLNPDVLLLAGFMQA
                     LVDAGHIHSRVGSVCGKLLTMRATFDIPDRPLVDSTGIYFNPMLRHLDRGSQEVDNGH
                     FLQYEYVFGASAAAALYRRKMIEDVAIGGEFFDPDFFVYREDADVAWRAQLLGWRCIY
                     TPHARGYHVRKVLPGNRRALPPVINMHSVKNRFLMRIKNMTGDLYWRNFFSIMTRDTV
                     VLGCCLLREHSSLQAFWYLAKNWRRVWEKRREIMRRKRVNDEYIASWFRYLPVSRPAP
                     KAPAHVVTKSRAVRG"
     gene            15682..16767
                     /locus_tag="VLE22_22560"
     CDS             15682..16767
                     /locus_tag="VLE22_22560"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HSB17250.1"
                     /translation="MNLAILGTRGIPARYGGFETFAEELSTRLAARGHRVTVYCRQRH
                     PESEYRGVALRYVPTLRYKYFDTIAHTVLSSLDLLVHRQDAALYCNAANALFTFAPRL
                     AGIPVALNVDGLERKRRKWNRMARGWYLLSEWLATFFPNRVVSDARSIAAYYRTRYGK
                     ETDFIPYGAETGKVATTEVLGRLGIEPGGYFLYVSRLEPENNALLVREAFERVTTEMK
                     LVLVGDAPYAAEYISRVRNTQDARVILPGAIYGQGYHELTSHCFAYIHATEVGGTHPA
                     LIEAMGRSALALYLDTEENAEVAGGAGIPFTADNLSAVLEHVLRMEDSERDQWRAAAE
                     ERVRERYSWDAVTDAYEELLLGLASTR"
     gene            16775..17902
                     /gene="alr"
                     /locus_tag="VLE22_22565"
     CDS             16775..17902
                     /gene="alr"
                     /locus_tag="VLE22_22565"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSB17251.1"
                     /translation="MSQPYRSWVEVSLGQISENFRAVRTAVGSGVEVMAVVKADAYGH
                     GAGEVSRRLEADGARWLAVSNVGEGAALRRAGIGARIVVMADFLPFEREALLEHDLTP
                     VIHSLADVAEFDRFAARVGAPLCYHLKLDTGMGRLGTQAPAREIAAAVQAARHAQLEG
                     LMTHFASSADYTSQQTDRQVEAYRNVLATLRADGVAPVWLHLSSTNSIAFSRREAWGN
                     MVRAGHAIYGYVSPARGEAPKRILNVKPALSWKATILALKDVPEGALIGYGGMFRAPR
                     PMRIAVLAVGYADGFPHRLSNKGKVIAGGRLAPVIGAVSMDLTTIDVSQSPEVKPGDA
                     VTLLGSEGDVSLDAQQIARAAGTISYSVLCGINVRVKRVYV"
     gene            complement(18012..>18784)
                     /locus_tag="VLE22_22570"
     CDS             complement(18012..>18784)
                     /locus_tag="VLE22_22570"
                     /inference="COORDINATES: protein motif:HMM:NF013177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17252.1"
                     /translation="RYIKDEKEFMREILRRATENVVVEVSSHNGSPSQRLEGTELRNF
                     LMALEEYLQISSKVERKLRDSRAVTVVSDPELKIDSKSDFGDENNLKPLARALEAAGV
                     PAELKHDEEHSSWMVSFRDSTHAERTIGVELAAMPEYRRLRSLGRQIGRYNQPPFVVV
                     KDSSRVIQEHWRGLLDYVKEEGKKDASVQRYKGLGEMNADQLWETTMNAEKRTLLRVR
                     LEDAVECEEIFSTLMGEDVEARRKFIEENALDVRNLDV"
BASE COUNT         3593 a         5624 c         6017 g         3550 t
ORIGIN      
        1 aagatcgacc ggccgcagat cgactgccac atcgcctaca cgaccgacga aacgcatcgc
       61 attctgcgcg acaacatctc gcgctcaccg ctgtacagtg gccgtatcga aggcgtcggt
      121 ccgcgctact gcccgtccat cgaggacaaa atcatcaagt tccctgagaa gatacgccat
      181 cagatttttc tggagcccga agggctcgac acgtacgagg tctatgtcaa cggcatgtcc
      241 accagcatgc cgatcgatgt gcagacagcc atggtcgctt cggtcccagg gcttgaagac
      301 gccgaaatga ttcggcccgg ctacgccatc gagtacgacg ccatcgaccc gcgcgagttg
      361 gaccatactc tcgaagtgaa gtcgatccgc ggactgtacc tcgcaggcca gatcaacgga
      421 acgtccggct acgaggaagc cgcctgccag ggaatcatcg ccggtttgaa cgctgcgtgt
      481 cgtctcggag gaatggaacc cctcgtcatc gggcgcaatg agggatatac cggaatcctc
      541 atcgacgacc tgatcacgaa aggcgccgag gaaccctacc ggatgtttac ttcccgcgcc
      601 gagttccggc ttcacctccg catcgacaac gccgacgaga ggctcacgcc ggccggccgc
      661 cgcgtcgggc tggtcactga ggaacgctgg cggctctaca cccgcaaaca ggagcagaag
      721 cagcgcctgc tgccgttgct ggaacaaact cgcgtgtcgc cggacatcct cccagatgtc
      781 aacctgccgt ccggcgaccg ccccaccctg gctcaatggc tgaagcgtcc cgaagctcgc
      841 atcggtgcgc taacgggatg gatcgccggc atccttggtg agccgccggt gcgcggagtt
      901 cttacgacgc tcgagacgga aatcaagtac gacggctata tggcccagca ggagcggcag
      961 atcgagcgac tcaagaacgc cgagggccgc cgtatcccgg acacattcac ttaccacaat
     1021 gtgccgggtc tctcgctcga ggttcgtgac aagctccagc gggtccgccc ggcaaccctc
     1081 ggccaagccg gccggatccc cggcgtcacc ccagccgcca tcgccgtcct ggatgtatac
     1141 ttgacccttg cgcgcgaaaa tgcgtcataa tcggcgcatt tttgtgcgtt ttgggagcat
     1201 tttgtgaccc gttttcgcac gctcctggac gagaacctct ccggaatctg cacgctgacc
     1261 aacgcccaga tcgagcagct ttatggccac ttcaagcttc taagtcattg gaacgaaagg
     1321 attaatctca cttcgatccg ggatgtcgac actgctgtcc ttcgccatta ttgcgagtcc
     1381 ctttttttcg cctcccgcct gcctaagtcc tttgtttcca tcgccgacgt cggctccggt
     1441 gccggttttc ccggcatccc gatcgccgtc ctgcgcccgg actgccgggt cacactgatc
     1501 gaatccgttc aaaagaaagc acttttcctt cgtgaggcga cgcgggggtg ggagaacgtg
     1561 cgagtcgtcg cccggcgggc cgaggaagtc gaggaacagt tcgatctgat cgtggcgcga
     1621 gcggtttcac cgaaaggggt tgtacggctt gtgccgaggc tagcgccggc ggtggggctg
     1681 tcgatcgggg aagataatct ccataagcta ttgaaaataa aagatataat ctgggatcat
     1741 cctgagaagc taccgtgggg agagcgtcga gtgattctga tcggacgtca ggtttcacgt
     1801 ggaacgtgag tgtgttcgta aatgggattg gctttgttcg gtctacgtct gggcgttcca
     1861 cgtgaaacac cagccgtgat acgatgagtg gaatcattcc cgggagtctg attcttgggt
     1921 aaggtcatcg cgattgcgaa ccaaaaaggc ggcgtcggca aaacaacgac cgccatcaat
     1981 ctggcggctt ctctcgccgc caacgacctg cgtgttctcc tcgtcgatgc ggatccgcag
     2041 ggtaactcca ccaccggact tggcgtttcc aaggaccacg agcgcgctag cctgtatcac
     2101 gcgctcatgg gccagcggga gatggggtca attacggtgc agacggattt cttcgggctg
     2161 gatctcattc cggctgacag aaacctcgtc ggtgcgaacc ttgagctgat tgaattcgag
     2221 gggagagaaa cccgcctgcg ccagacacta caaacattgc gggataagta cgaatacatc
     2281 ctgatcgact gcccgcccgc gctagacctg ttgacgctaa acgcgctcgt ggcagcggat
     2341 tcggtgctaa tcccgatcca gtgcgagttt ttcgccctcg agggcatttc ccagttgctg
     2401 gacacagtcg agcgcatccg cgagtctttc caccacccgc tgaaaatcga gggggtgctg
     2461 ctcacgatgt ttgacgaccg gacgaatctg acccgccagg tcgccgccga tctgagggag
     2521 ttcttcagcg acgaagtctt caagactgtg attccccgga gtgtccgact ggctgaagct
     2581 cccagttatg gcaaaccgat tcttgcctac gacatccggt cccgcggagc ggaaagctac
     2641 atccggctcg cgaaggagat cctagcgaat gaccaagacg tcagaacacc cacggaaggc
     2701 gctcggtaag ggcctgtcgg cgctccttgc gccccgcgca ccccagcttg cttcggcccc
     2761 ggccccggta cctcggcgtc ccgagggtga agaggttttg cggatccccg tcacgcagat
     2821 tgacccaaat ccgcttcaat cacgcacgat attccaggcc gagaagctcg atgagctcgc
     2881 acagtcgatc aaggcgaatg gcgtgattca gcctctcatc gtgcgccggc acgagaaccg
     2941 ctatcaactc gttgccggtg agcggcggtg gcgcgcgtcg aagatcgccg gcttgaccga
     3001 ggtgcccgtc gtggttcaga acctgaccga cgagcagctt ctggaagtca cgctgatcga
     3061 gaacatccaa cgcgaggacc tcacgccgat cgaagtcgcg cacgcgttcg agcgtctcgt
     3121 gagggaactc ggactcagtc atgacgaaat cggcaggcgg acgggaaagg atcgcagcac
     3181 gatcaccaac ctgcttcgtc tgcttcgtct gccgccggac attcagcaac ttctcacgga
     3241 acaccgactc tcgatggggc atgcacgggc gattctcggg ctgccaagtg aggaattgca
     3301 gcgccaggtc gcagagaagg cctcggcgca agggttatcg gtccggcagg tcgaacggct
     3361 catccagcga atgacggagt ctagggaaac caagtctccc gatgaggtat cgatcgaccc
     3421 gaacgtcaaa gccgcaattg acgagctgga acgggtgctc ggcacgagag ttcggatcca
     3481 gcagaaatcg gatcatcgcg gccgcataga aatcgaatac tactcaactg acgacctcga
     3541 ccgaatctac acgctcatcg tcggagactg atgcgctcgg gatggtgccg gcggctggaa
     3601 tcgaaccagc gacctaggga ttatgagacc ctcgctctag cctctgagct acgccggcgt
     3661 cgtgggctct ctattcagcg tattcaccgc caggcgattc tgtcaacttc ggatgctcgg
     3721 atccacggtc gcttgacaag ggcaggctac cgggtccata ttgagggtgg aagacggtcg
     3781 agagcggctg gattccggtt ccctccatcg ggagggagga tatcacggct ttttccttcg
     3841 ggagacggtc gtaacaaagg aggaagtcca tagagaacgt ccacaattgg gggcgactac
     3901 cggctttctc cgctggccaa ctgtgaacct gaagcgccgg agagccattt gaaggctaga
     3961 gcaggcgccg cggaacgcgc accgcaaaag taaggtagca cacagttgga ggatgcggtt
     4021 tcggttacgg caaattggcc ggccggggct ggcatcgcag cagagtgtcg gcggtcgccc
     4081 tttcgtttgt ccggacgttt gcgccgtgcg ttgacacttc ccctcgccct ccagtagatt
     4141 cgtagttgcc ccgccttccg agcgaggaaa taacttcgtg acttccgtcg agtttcaagg
     4201 cgtctccaag agctatccaa tctacgcctc tccgggcgac cggctcaagg aacttctgac
     4261 gctcaaccgg cggtcgtttc accgcgattt ctgggctctc cgcgatgtgt cttttgaagt
     4321 gcgccgggga gagacctttt gcgtaatcgg agagaacggt gcgggaaaaa gcacgcttct
     4381 tcagatggtt gcgggtattc tgcctccagc gtcgggtacc gtgcgagtgg aggggagggt
     4441 ctcggcctta ctcgaattgg gctccggatt caacccggaa ttcacgggac gcgacaacgt
     4501 ctatctgaac gctgcgattc tcggattttc gcgacagcag atcgaccgta agtaccggga
     4561 gatccaagag ttcgccgaaa tcggtgattt cgtcgaccaa ccggtgaaaa cgtactccag
     4621 cggaatggtc gtccgccttg cgttcgcggt ggcgattcac gttgatccgg agatcctgct
     4681 cgtcgatgag gcgctagcgg tcggcgacat ttacttccgg cagcggtgca tgcggaaggt
     4741 ccacgaactc agatcgcgcg ggatcaccat cttgtttgtt tcgcacgcga tcgccgatgt
     4801 taaggcgatt ggcgatcgta ccctctggct cgaagagggc caggtcaaag atctgggcac
     4861 gacggacgcc gtagtggccc ggtatctggc ggcgatggcc gagaaggact ccgcatactt
     4921 gaacctcaca catcggccgg caccggagtc tagcagggcg cagtatcggg cgcccgagat
     4981 cgtcgaaacg atcccgaaca tcgatcaccg gcacggggac ggacgcgccg aagtcatcgg
     5041 aatcgctgtt ttggactcca ccggccaccc gttgccgtta cttgagcccc agtcgcgggt
     5101 ggtggtgagg atcagcgtgc gggcgaaaga ggagatttca ctgccagtgg ttggctttat
     5161 gcttcggaac cacctcggta tcgatttcgc cgggttcaat accgcccggg aggggatcga
     5221 acttccgccg atgcgcccgg gggacattca caccgtcgat ttccatgtcg atttcccgga
     5281 gctgtatcca ggaacatttt cgttctctcc ggcgatcgcc gacggaccgc tcgtgagcta
     5341 tcgcacttgc gactggatcg acaacgccgt cacgctgcaa atgggccaca gcggcggcca
     5401 ggtctacggc tacatgcgca tcccgtgccg ggttacgctg aacagccggt tgcactccga
     5461 ggcccgcgaa acggagacca aagttggctg aatttacggg cgagcgagtc atcccagggc
     5521 aggtagacgg agatctttgg aatgagcaca tcgcacgtta cgccttcgcg tcccggctgg
     5581 cccgtctcaa acgggtgctc gacgcgggct gcggatcggg ctacggagca gcggacttgg
     5641 cacggtgcgc agcgacagtg gtgggagtgg acaattcagc agaggccgtc gcctacgccc
     5701 gcgcacacta tccgcttcca aacctaagat tcctcgaagg ttcctgctcg tcattgccgc
     5761 tagcgacagg gtcgttcgat ctggtggtgt cgttcgaggt catcgagcac ctgcaggact
     5821 gggcgacgtt tctgagcgag gtgcgccgcg ttctcgctcc gggcgggcag gcaatcatct
     5881 cgacgccaaa cagccagtac tatacggatt cgcgcggaga aagcggcccg aatccgtggc
     5941 acgagtatga gttcgccttc gaagaattcc gcgacgagct tctcaaggtg ttcccgcacg
     6001 tgtcgatgtt cgtgcagaac catgtcgagg gcttcgtctt ccaacccgtg aagacattct
     6061 ccaccgcgga agcgagggtc gagagtggag ggggcggacc cgaggactcg catttcttcg
     6121 tggccgtctg cgcgctgtcg ccgcagaccg gagcaccaac gttcatctat ctgccgaagg
     6181 cggcgaacat ccttcgagag cgcgagcagc atatcgtcaa gctccagaag gagatggcga
     6241 tcaaggacca gatgctgctc gacatggaga aggagctcaa ggaacgctcc gaatgggcgg
     6301 aacgactcaa ctccgaactg cgagaggcgg gagcgacgat cgagcggctt catcgcgaac
     6361 tgggcgagat gtcgcgaggc tacgaagcaa aggtcggcga actcgaggag gagaaccgaa
     6421 aacgcgcgga atgggctctg gagaccgaac gccggctcga agacaaggtg caggagcttg
     6481 cgaagtgcgt cgacgttctg cacgagacgg agaagacgct cgatgagcgg acgacatggg
     6541 cgttcggctt gcaaaagcag gttcgcgatg tcgagtacca gcttgccctg gtccggggct
     6601 cgcggtggat gaagctcggc aatgcattcg gcatcgggcc gccgctgccg aaagagtgag
     6661 atgacggtct tcccacgcgt gctgcgggcg ttgccgctgc tgttgatctc gccgctgctg
     6721 attctgctga cagccgcggc gctggtggcc gctgatctgg tctggttcct cctggcgcgc
     6781 agatggcttt taccagaaac cgggccggcg aagcaagcca cctcggtagt aattcccaac
     6841 tggaacggcc gcgacctgct ggaagaatac ctcccctctg tcgtcgccgc cctggaggcg
     6901 aaccctgaaa acgagatcct cgtagtggac aacgcttccg aagatggcag cgccgcttgc
     6961 ttgcgcgagc gttttccaca cgtaagggtg ctcgagatgc cgcggaacct cggcttcggt
     7021 ggcgcatgca acgccggagt catggcggcg gcgaacgata tcgtcgtgct gctgaacaac
     7081 gacatgcgcg tggagccgga tttcctgagg ccgctgctgg aaggttttgc ggatgagaag
     7141 gtgttcagcg tctcctgcca gatcttcttc acgaacccgg agaaggcccg cgaagagacg
     7201 gggctgacac agggatggtg gcagagcggt gcgctgcggg tccgtcaccg gatcgacgag
     7261 ggcgtgaaga cgctctaccc gtgcttttac gggggcggcg gctccatggc ggtcgaccgg
     7321 cggaagtttc tggagctcgg cgggttcgac cgtctgctcg aaccgttcta tctggaggac
     7381 acggacctcg gatacatggc ctggaagcgc ggctggaagg tgatgtacga gccgcgcagc
     7441 gttgtctatc acgaacaccg tggcacgatt gggcggcggt tcagcgcacg agagatagag
     7501 cttgtccttg aaaagaactt tatactttgg agctggaaaa acatccacga gtggccccgc
     7561 ctggcgtcgc atttcttcta ctgttacgcc ggcgcactcg caagcgtggt gttcggcaaa
     7621 tcgccggaac gggcgacgct tgagggcttg tggcgcgcgt gccgacaact tccgcaggct
     7681 gtccgttcgc gctggaacgc gcgggatctg gcggcggtca acgacaccga agcctttcgc
     7741 cgaccgctgg gcggatactt ccgggatcgt ttcgcgacgg tccccaccga gctggacagg
     7801 ctccgcgtgc tcttcgtctc tccgtatccg atcgttccgc cggttcacgg cggcggcgtg
     7861 ttcatgtacc agacgttgca ggaactcgta cggcactgtg aggtgcatct cgtcgtgcta
     7921 ctgcactacc cacacgagct ggcggcgcat gattgcctac gcgggctgtg cgcatcggca
     7981 gagttcctgg tgaagaagac agtcacgggc aacggagctg gatcgatcct gccgcacgcc
     8041 gtccgcgagt ttgccaacag cgatctggag tggctgatcc accgacagat ccacacgcag
     8101 gaaatcgacg tcgtccagct cgaatacacg ccgatgggcc agtatggggg cgactaccgc
     8161 cgggttgtaa acgtgctgtt tgagcacgac atttactttc agtcgatcgg ccgaggctgg
     8221 aagcaggctt tcggcgccgg gggaaagctc aaggcggcgg tggagtacct gcgggcgctc
     8281 cgctacgaga cgagcatcct ggagcggatg gaccggattc agacgtgcag taccgcaaac
     8341 cgggactacc tgctgtcctt cgcgccggac cttgacggcc gcatccaaga cggcctccgg
     8401 gcggggatcg acacctcccg atatcacttc cggccggatg gtcgcgaacc ggcaacgatg
     8461 ctttttctgg ggaacttccg ccacctaccg aatcggacgg cattggactg gttcatcgag
     8521 aacgtgctgc cgcgggttcg caagcagcgt ccggactgcc gactcgtggt tgtcggcagc
     8581 gaactgccgc ctcgacacgc cttttcgagc gtggagaaca tcgagctggc gggctacgta
     8641 gacgacatcc gcgggccgct ggagcggtac tcggtcttcg tttgtccgat cctcagcggg
     8701 tccggcgttc gcgtaaaact actggaggcc tttgccgcag gcattcccgt cgtctctacg
     8761 cgattgggcg cagaaggact ggcaagcgtc gacagggagt tctgcgctct cgcggacgat
     8821 gcggagacgt tcgccacaga catcctcgac ctgttcgatc atcccgaccg ggcggcgcag
     8881 atggccacgc gtgcgcggag tgaagtcgcg gagcgttggg acatggggaa ggtgaccgcg
     8941 aaactggcgg cgagctaccg tgaggccgtc cgggaaaagc gattcaccac agagagcacc
     9001 gagaacacgg aggactaatg gaaagacctc tccctgcggt cgcggctcct tatggcacag
     9061 tggggatcac ttcgccgaag tgacgtcctt ccagctttcc aaaattccgg ccggcgaatc
     9121 ggcgtaccgt acggtgaagt ttccgccggc acctagcatc gcgtatacgc cggcggagcg
     9181 gcgataaacc ccggtagcaa gcttttcgcc agcgaggcgt tgcgcctcgt ccaccggatt
     9241 cagcagtagc agtggccgcg gcgccaccaa cgccgccaag tccggcagat caaactcttg
     9301 cagcgcatcc tgcacgaagt cactcagtgg atgagagaag atctcacctt cgacaatgga
     9361 ggcgtacgac agcagcgcgc cagtcacggc cgcggagcga atacgatcgt ccagcgcggc
     9421 tgcgtacaag acgagtaggc ctcccgaacc gctacccacc gcggcgaccg ccccgcgctg
     9481 cacctctttg cgggcctcca gatagtcgac cgcccggaga acgtcatagg cgcgcatgcc
     9541 gagcacgttt ctgccaaccc tgagcgcgct gtagaaacgg ccggcctcct ccccggtgcc
     9601 gaggtcggca taatccttta ccagctcctg gcctgtttct ccggcgccgc gcacgtcgat
     9661 ggcgagcacc gcgcggcccg cttcgaccag aggccgcaga tatcgctcca ccactccacc
     9721 agcgctcttg ccattgtcgt tgacgaagat gatgccgggc gcgggcttca cgctcctagg
     9781 cagaagaagc agcgcgggca cgtaaatccc tggctcgctg tagtagacca gcttctcgag
     9841 ggtgtacgct cccttatcgg cgcgaaccag cgtgttcgcc tgaaggggtt cggcgctcga
     9901 cgggagtgcg atcttcccgg aaatagtccg gcgcaaccga gcctgccagc gctgccactg
     9961 ttcgcggccg gccggggcgg cggctccggc ccgcaaaccg cggaaccggg cacgattcaa
    10021 cgaagaaagc gtttcaccgc cgatcgacgt cctcacctgg ccggtgagtg tggccgccag
    10081 gtctgcttcc gtttccagcg cggtgcgagg ctcctccacg ctgtccgccg cgatcccggg
    10141 cagccagcgt gtgagccagc tatacgccac aggaaacatc gcgtcggtaa acccgtgcgg
    10201 accgtcgaag atgcggaatt gcgttcgctc agccgctccg gcgacctcat agagccgggc
    10261 gaccagctcg tgccggcggc gcttctccgc cacgctggaa cgcgaatcgc cggtcgcttc
    10321 catcatcagc agcgggcgcg gcgcgatgag ggcgtcgaca tcagccaccg tcatgctcgc
    10381 gcggaacagg ttgtctgcga tgcagccgcc ataacagacg ggtatcgcca ccctcaccct
    10441 cgggtcgtag gcggtgagga gttccgtcag ccatccaccg ccagatatgc cggtaacggc
    10501 gatgcgcttg ccgtcaacgt cattgcggga ttcgaggtag tcgagcccgc gcaacgcatc
    10561 ccacaacagg tacgaaacca ggttgcccga cgtcaggatg gttcgccccg cggccagccc
    10621 gtgctcggtg gtgacgaagt actccgacgt acccggatgg atgatcggcc ggcggaagac
    10681 gggatcccag gattcgacgc gctcgcccat cccgggaagg tcgtacacga gaacaaggaa
    10741 gccgcggcgt gcgaggtaca tcgccagccg ctgataatcc gggtaggcct tgcccgcgcc
    10801 ccagtggccc acattcgcca gcaccgccgg aaacggggct ttgaccctcc ggggcacgta
    10861 gacgttggcc gtgacataga aacgcggccg gctttcgaac aagagtttct cgacgaagta
    10921 gtcgccgcgg tcgagcgtgc cggtcacgtg ggagttgagc gggctgcgac cgggcagggc
    10981 gccgacggct gcccgcaggc gctcgcgagc agcggtctgg aaccgctcca ggtccgcggt
    11041 gttcttcacc gttgcgatag cgccttcatg ttccgcttcg acggcggcgg ttagcttgcg
    11101 cagatacgcg tccagcatgc ggtcggcctg ctcgaactcc gcccgaccgt agagcacgtt
    11161 ccactcgggg ctgaagtcca ccgcggactg cgcctccacg gtcgcaggcg cagcgttcag
    11221 aaggagtagc agcgggacca tgcgggcgaa aactctgttg gcgtccattg gtagattatg
    11281 gcgcgcagtc gcggctcgga agctgcgtgt gttacttacc gccggtgagg tcttttttca
    11341 gccgctcggc gattcggcgg gcggtgcggt ccatctgctc ggcggtggtg ttcttgggct
    11401 tttcgaaggc ggaccagatc acgttccggg tgtgggcatc gaccaggaac agcgtccccc
    11461 gcgcccggcc ggaactgaag agtcgaaccg gtctctccct cgtcttccga gcctcttcgg
    11521 tggcttcgga atccccggaa gtggcggtct ccttcttctc cgatgtctcc gctttctccg
    11581 gagcagcggc agccggcttg gtttccgccg gcgtctcggc aggatagagg tccgcgagac
    11641 gcttctcaaa gacttcgcca atctggtcgg taagcacggc gtcagccttc ttcggatcag
    11701 tcaccacctg gacgacacct tccgaagtca gccggttcgc aagaaactga tcgaggccac
    11761 ccgtcatcgg gaagagatat accgactgga ccttgggcgt ctgaacaccg gcgatgggaa
    11821 gcgacgccat caaggcgatg cagcataata ggaggacgac cggcttcatg gataccccca
    11881 tcatacagca tgtataagga cctgacggtt accgtaatca ttccgtgtct caacgaagag
    11941 cagggcgtag aacaagttct gaagagcatg cccgcattcg tcgacgaggt gatcgtcgtc
    12001 gataacggtt ccacggatcg cacatccgac gtcgcccgct tccacggggc gaaggtgatc
    12061 cgcgaggaag tacgcggtta cggacgggcc tataagagag ggtttgctca tgcgacagga
    12121 gatctcatcg tcacgctgga cggcgaccat agttatccgg tggatgcgct ctcctatctt
    12181 ctggaggcct ttctgcatct cgaggtcgat ttcctgaata cgtcgcgctt ccctgtccgc
    12241 gaccctcgtg cgatgagtct caaacactgg gtggggaata tcatcctgtc gctggtgatg
    12301 tcggcgttgt tcttccgttg ggtccgggac tcccaatccg gtatgtgggt gctccggcga
    12361 tcgattctca atgacatgca cctcgagtcc gacgggatgg ccttctcgga ggagatcaaa
    12421 gtcgaggcga ttcgcaatcc acgaatccgc ttccgtgaga tcacgattct gtactcgtcg
    12481 aggcttgggg aggtcaaact caatccgtgg cgcgacggaa tccataatct gctctttctg
    12541 gtcaagaaac gatttgcgcg aaagaggaga aagtgataat atcaaggctc acccgcctgc
    12601 gttccgattc ccaattccca tgaacggtgg gcctgtccgc agtatctccg gagtcaccgt
    12661 gatcgtgccg acttggaacc ggcgcgagct gctggagcgg gttctccaaa gccttttccg
    12721 gcaaacgcac ccaattcggg aaatcctggt agtcgataac ggttccgagg atggttccgc
    12781 cgaagccgcc gagaggcagg gcgcgtgtgt tatccgaatt gggtgtaact ccggctttag
    12841 ccgggccgtc aatcgtggca ttggcgaagc aaggacggaa tggctcgcaa tcgtgaacaa
    12901 cgacgtggtg ttggagcccg actggctctc ccggctgatc gacggatcga cggggaacga
    12961 ggtctggttc gccaccggga aactgttgcg agagggccgg cgtgattcga tcgacggcac
    13021 ctatgacgcg atctgccggg gcgcttgcgc ctggcgtgtg ggccacgcca ggcccgacgg
    13081 gcccgagttt gacgagagcc gtccgatacg gctggcgcca ttcacagcgg cgttgttccg
    13141 gtctgaattg ttcgcgcggg taggattact ggatgagagt ttcgagtcgc acctggagga
    13201 cgtcgatttc gggttgcgct gcgcaagcgg cggatacggc ggcgtgtatg tgcctagagc
    13261 ggtcgccgtc cacacgggaa gcgcgaccga gggtgcgtgg aaccgtgaga cggtgcgcag
    13321 gatcgccaga aaccagttgc ttctggtggc caagcactac ccgagtaact ggctggttcg
    13381 ctacggttgg ccggtactcg ttgcgcagac actgtgggga ttcgtggcgg cggcgcatgg
    13441 agggggaacg gcctacctcc gcggcaaact cgagggggcc cggcggtttc gcgggttgcg
    13501 gcgcgacgga atacgcggcg ggatggtgga tccagaaaag cttgctcgag ttctcgagga
    13561 aagcgagcag gaaattctcc ggctccagcg gcgcaccgga tttgaccggt actggcggct
    13621 gtattttgca ctgacatgag ggaagcgggc gcagtcatcg tgacatacaa ctcgggagcg
    13681 gaaatcggac cctgtctgga tgccgccatc ccgcgcgccg gcgaagtggt cgtggtagac
    13741 aacggctcga tggacaacac actggaagaa gtccggaagc ggctgtcggt gaagctcatc
    13801 gtcaattcgc agaaccgggg attcgcgggt gcggtcaatc aagggttcgc tgcgacagaa
    13861 tcgccctacg tcctgctgct gaaccccgac gcggttctcc tgaccggcgt ggagcccatg
    13921 gccgccgcgt gcgcgaggcc cgggataggc gccgccggcg gaaaactcct ggacgcggac
    13981 ggcagagccc aggacggatt catggtgcga cggttcccga ccgccctagc gctgattctc
    14041 gaggtgctcg gagtgaaccg gctttggaag aggaatcctg tgaaccggcg ataccgctgc
    14101 ctggacatgg accccgacgc ggccgcagaa gcggaacagc cggcgggcgc cttcctgatg
    14161 atccggcgcg acgcctggca ggctgccggt gggtttgacg aacagtttca cccgttgtgg
    14221 ttcgaagacg tcgattttct caagagactt cgtgacaagg gcttgcaggc ggcttacgag
    14281 ccagcggcgg cggcgcgtca cgagggggga cactcaatcc agcgcctgtc ccaggagtcg
    14341 cgaattcttt attggtatgc tagccttcta aggtatagtt cgaagcactt tactccgtcc
    14401 cggctcgcgg cggtatgcgt ggcaacggcg ctgggttctg tattgcggat gatcggtgag
    14461 tccgcgctgt cccggaacct caggccattg cgggtgtata gtaaagtggt tcggcttgcc
    14521 tgcgttcggc tgatgctgtg ccgattcgcc ggaacgggca gttcgccggt cctggcgcgg
    14581 cattgaagcg gagagatccc gatacccggt ttcgacacaa taactcacgc gcatggcaca
    14641 taacgatctc gtttccgtca caatcgtgac ttacaacagc ggccggttca tcaggcgttg
    14701 tctggagtcg gtactcgcgc agaaataccc gaacaaagaa attatcgtca tcgacaacgc
    14761 atcgacagac gggacgatcg atatcctcga gcaattcgag gatcgctgcc agattgtgta
    14821 caacgaggaa aacctcggct tcgcggcggc gcagaatcag gccatcgacc tctcgacggg
    14881 cgagtgggtg atgacgctga acccggacgt gctgctgctg gcgggcttca tgcaggcact
    14941 ggtggacgcg ggtcacattc actcgcgggt cggatcggtc tgcggcaagc tgctcacgat
    15001 gcgggcgacg ttcgacattc cggaccggcc gctggtggat tccacgggga tttacttcaa
    15061 cccgatgctc cgccatctcg accgcggcag tcaggaggtc gacaacgggc acttcctgca
    15121 atacgagtac gtattcggcg cgagcgcggc agccgcgcta taccgccgca aaatgatcga
    15181 ggatgtcgcg atcggcggcg aattctttga tcccgatttc ttcgtgtacc gtgaggacgc
    15241 cgacgtggcg tggcgggcgc agttgctcgg ctggcgctgc atctatacgc cgcacgcgcg
    15301 cggctaccat gtgcggaagg tgctgcctgg caaccgccgg gctttgccgc cggtcatcaa
    15361 catgcactcg gtcaagaacc ggttcctgat gcgaatcaag aacatgaccg gggacctgta
    15421 ctggcggaat ttcttctcca tcatgacgcg ggacaccgtg gtgttgggct gctgcctgct
    15481 gcgggagcac tcctcgctcc aagcgttctg gtacctcgcg aaaaactggc ggcgggtgtg
    15541 ggagaagcgc cgggagatca tgaggcgcaa gcgggtgaac gacgagtaca tcgcgagctg
    15601 gttccggtac ttgcccgtga gcaggcccgc gccgaaagcg cccgcacacg tcgttaccaa
    15661 gtcccgcgcc gtgcgcggct gatgaatcta gcgattctcg gcacccgggg cattcccgcc
    15721 cgttacgggg gattcgagac ttttgcggaa gagctctcca cccgccttgc ggctcgcggc
    15781 cacagggtaa ccgtctactg caggcaacgc caccctgaat ccgagtaccg cggcgtcgcc
    15841 ctgcgctacg tccctacgct gcgctacaaa tacttcgaca caatcgccca tacggttctt
    15901 tcttccctcg atctgctggt gcacaggcag gatgcggcgc tatactgcaa cgccgcgaac
    15961 gccctgttca cgtttgcacc acgcctggcc gggataccgg tggcgctgaa cgtcgacgga
    16021 ttggagcgga agcggcgtaa gtggaaccgg atggcgcgcg ggtggtatct gttgtcggaa
    16081 tggctggcga ccttctttcc caatcgtgtg gtgagcgacg cgaggagcat cgccgcgtac
    16141 taccggacgc gctacggcaa ggagacggat ttcattccgt acggcgcgga gaccggaaaa
    16201 gtcgcgacca ccgaggtact ggggcgactc ggcatcgagc cgggcgggta tttcctctac
    16261 gtgagccgac tggagccgga gaacaatgcc ctgctcgtgc gcgaggcctt cgagcgcgtc
    16321 acgacggaga tgaagctcgt gctggtgggc gacgctccct acgcggcgga gtacataagc
    16381 cgcgtacgga acacacagga cgccagggtg attctcccgg gtgcgatcta tggacagggc
    16441 taccatgaat tgacgtcgca ctgtttcgcg tacattcacg ctacggaagt cggcgggaca
    16501 cacccggccc tgattgaggc gatgggccgc agcgcactgg ctctgtacct cgacaccgag
    16561 gagaatgcgg aggtcgctgg cggcgcgggt atcccattca ccgcggacaa tctcagcgcc
    16621 gtcctggagc acgtcctgcg aatggaagac tcggagaggg accagtggcg ggcggcggcg
    16681 gaggaacgcg tccgtgagcg ctacagttgg gacgcggtca cggacgccta cgaggagctt
    16741 ctgctcggac tcgccagcac ccgataatgt aagaatgtcc cagccgtatc gttcctgggt
    16801 ggaagtttcc cttggccaga tttccgagaa ttttcgcgcc gtgcgcaccg ccgttggatc
    16861 gggcgtcgaa gtcatggcag tagtcaaggc cgacgcctac ggccacggcg ccggcgaggt
    16921 ctcgcggaga ctggaggccg acggggcacg atggctggcg gtaagcaacg tcggggaggg
    16981 cgccgcgctt cgccgggcgg gcatcggcgc gcggatcgtg gtgatggcgg atttcctgcc
    17041 gttcgaacga gaggcactac tcgagcatga cctgacgccg gtgatccatt cgctcgcgga
    17101 tgtcgccgag ttcgaccgtt tcgccgcccg cgtgggcgcc ccgctgtgct atcacctcaa
    17161 gctcgatacg gggatggggc gcctcggcac gcaggcgccg gcccgtgaga ttgcggctgc
    17221 ggtgcaggca gcccgccacg cccagctcga aggcttgatg acgcacttcg cgtcctcggc
    17281 cgattacacg agccaacaaa cggacaggca ggtcgaagcg taccgcaacg tgttggcgac
    17341 cctgcgcgcc gacggcgtcg ctccggtgtg gctgcaccta tcgagcacga actcaatcgc
    17401 attctcgcgg cgagaggcgt ggggcaacat ggtgcgggcg ggccatgcca tttacgggta
    17461 cgtgtcgccg gcgcgcggcg aagctccgaa gcggatcctg aacgtgaagc cggcgctttc
    17521 ctggaaggcg acgatccttg ccctgaagga cgtgccggag ggagcactga tcggttacgg
    17581 cggcatgttc cgcgccccgc ggccgatgcg gatcgcggtt ctggctgtgg gttacgcgga
    17641 tgggtttccg cacaggctgt ccaacaaggg gaaggtgatc gccgggggca ggttagcgcc
    17701 ggtgatcggg gcggtatcga tggacctgac gacgatcgac gtgtcgcagt caccggaggt
    17761 gaaaccgggg gacgcggtga cgctcctcgg cagcgagggt gacgtctcgc tcgacgcgca
    17821 gcagatcgcg cgcgcggcag ggacgatttc atacagcgtg ctctgcggga tcaacgtgcg
    17881 ggtgaagcgg gtgtacgtgt agggggaaaa aggaaaacct ggggacagac accaatttcg
    17941 caaatacagc gaaattgaga gtcagtcccc ggttttccga tgaccgtcgt ggcactgttt
    18001 tggagttccg gttagacgtc gaggtttcgg acgtcgaggg cgttttcctc gatgaatttg
    18061 cggcgagcct cgacgtcctc gcccatcagc gtcgagaaga tctcctcgca ctcgaccgcg
    18121 tcctcgaggc ggacccggag gagcgtccgc ttctccgcgt tcatggtggt ttcccacaac
    18181 tggtcagcgt tcatctcgcc gagtcccttg tagcgctgga cggaggcgtc cttcttgccc
    18241 tcttccttga cgtaatcgag caggccgcgc cagtgttcct ggatcacccg gctggaatcc
    18301 ttgaccacga caaagggagg ctggttgtat cgcccgatct gccggccgag cgaccgcagg
    18361 cggcggtatt cgggcatggc cgcgagctca acgccgatgg tccgctcagc gtgcgtcgaa
    18421 tcacggaagg aaaccatcca tgacgaatgc tcctcgtcgt gtttcagctc ggcggggacc
    18481 ccggccgcct cgagcgcgcg tgccaacggc ttgaggttgt tctcgtcgcc gaaatccgac
    18541 ttggagtcga tcttcagctc ggggtcggag acaacggtta ccgcgcggct gtccctcagc
    18601 ttgcgctcca ccttggacga gatctggagg tactcctcga gcgccatgag aaagtttcgc
    18661 agctccgtgc cttcaagccg ctgtgacgga gagccgttgt gcgagctgac ctccaccacg
    18721 acgttctcgg tggcgcgccg caggatctcc cgcatgaatt ccttctcgtc tttgatgtag
    18781 cgtt
//
LOCUS       DATIFQ010000451        31081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1301719, whole genome shotgun sequence.
ACCESSION   DATIFQ010000451 DATIFQ010000000
VERSION     DATIFQ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 31081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31081
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301719"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VLE22_22575"
     CDS             complement(<1..1227)
                     /locus_tag="VLE22_22575"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB17253.1"
                     /translation="MQRISRWGMTVLCFALLIVFTSFALGQESRGRVQGTVTDPTQAA
                     IVGAKVTLRNVNTGIESAKETDTTGSYRFDFVTPGSYSVTVEASGFSKFVQENITVLT
                     AGDVTVNAPLTVGGVTEAVTVSEAVTAVQFNTSTMTTAVQGTMLQDLPVLARNPFTLA
                     LLNPAVVNQYWDVSHRNPFYMWSNSGLDVGGSTGGKNDQLLDGVPTGVAARGSYNSPM
                     DAVQEVVVQQNAVDAEYGFSAGGVLNLSMKSGTNDFHGSAYWFGRNPVFNAVVDPITR
                     TESVVKNNIWGGTLGNPIKKNKLFNFFSYERWKATQPSSNVSTVPTDLERTGDFSKTF
                     TPDGNLRMIYDPFSTQFDPATSTVTRTPFTNNRIPAQMIDPTGQKIINDLWKPNRTGD
                     DPSGVNNFKIGYGWWIK"
     gene            complement(1511..2839)
                     /locus_tag="VLE22_22580"
     CDS             complement(1511..2839)
                     /locus_tag="VLE22_22580"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17254.1"
                     /translation="MIAGVLTLALQVASPPAKSVSPQEHLRKGSSLLREGRIQQAEAE
                     FRIAVRAAPGNAVAHSLLGMALTGGQKLEEANVHFEAALKLDPSDAGTRYNLALNQFR
                     LGRSPDSRKHLERILKAKPGHPQASLLLGSVLESLGEDEAALKLLESLPGLVPKTPDA
                     ILSLARCYYRAGRFEECRSTLLATIAGGSRDPRLYRLLAWAYQRQVQPAEAVRTMMQA
                     IEIEPSNHTGYGELAALLIEQRRYSEAYDLTLRTVERMPKSALAYKRKGQAEGYIRSL
                     HEAVGSLTRAVELAPDDDEALLLLGQAQAAGWLISQASATFEKGIARYPRNARFYEAY
                     GRMLVQPTAKPDAAAEARAQSLLKKALALDASLPGARYELGKLLLKQGKPAEALPHLE
                     AAAKLSPGDSEVFFTLAGAYRDLDRDSDFRKTLGMYKALKAKEQPAEPVR"
     gene            complement(2883..4538)
                     /locus_tag="VLE22_22585"
     CDS             complement(2883..4538)
                     /locus_tag="VLE22_22585"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB17255.1"
                     /translation="MQTKPIPGVCRLFWSLCLATSVSSAQPPPIRFRNVAEAAGLRFV
                     LENNPTPEKQIIETMAGGLAVFDYDGDGLMDIFFTNGASVPSLEKKDPKFHNRLFRNL
                     GGMKFKDVTSEAGLAGSGYSIGASAADFDNDGHTDLFVAGVRSNHLYHNLGNGRFEEV
                     TAKAGIKSDLWSIAGGWLDYDNDGRLDLFVVNYLDWIPDKAPFCGDSVKNVRAYCHPK
                     HFRGLPNTLYRNRGDGTFEDVSEKSGIAHHVGKGMSVALADYDLDGFVDVFVTNDKIP
                     NFLFHNLGNGKFEEVALESGVAMADHARPISAMGADFRDYDNDGLPDIVVAALAGEWF
                     PLFRNLGRSRFRDWTDRSSLSRLSIDRSGWSPLLYDFNNDGRKDLFVSGAHVVDNVEY
                     FEPTKYKVPNCIFLNTGNGSFRDVSGEAGPDFQTPRAHRGAAAADFNNDGRLDVVVAV
                     LGGPPELWENTGPSVASWLVLRLHGARSNKDGIGASVRIGTQHNHVTVNGGYASSSNF
                     GVHFGLGGVAKATDIDIRWPSGVKQVLAEIPVNQVLQVSEPPD"
     gene            complement(4507..6381)
                     /locus_tag="VLE22_22590"
     CDS             complement(4507..6381)
                     /locus_tag="VLE22_22590"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSB17256.1"
                     /translation="MMPGSSPWEILLFLALLTASALAFWRRFGRVAAVIRRSRRDPDF
                     QLRPLLPRMRAFFGQVMLQERVIRDRPLPGIAHAFVFWGFCAFALVTINHLVEGLGLE
                     LIPRDSGFGQLYFIVAAVFAVAAGLSIAGLAIRRFVARPKWLGEISPESGLVAFLIFF
                     LMATYLTTFFVTEGSPAARINWWAHTLALLVFLPLIPHTKHLHLLLSPVTVFLKRPSF
                     SRIPPLAGDEDFGLDTGRDLTRIVTLQAYTCVECGRCTEHCPAYNTGKVLNPKEIILG
                     VRRYLNENGAVAEAPLIGRHLSQEAAFQCTTCGACEYQCPVGIQHLPVIVGLRRGAVN
                     TGKWDDDHGNKLFLNLERYGNPLGFASSERDKFIEKHGLPLYDGAQEYCLWLGCMGSY
                     DPQGREIVLAMVTVLRYLKVTFGVLKKERCTGDSARRLGNDLLFSQLAEANIEEIGRS
                     KARRLVSICPHCVRTISEDWKEFGADIEIEHHSEFLARHRDRLPAASGPRNTVVFHDP
                     CYLGRYRGTYEEPRDVIRLYGETVEPRRTRSSSFCCGAGGGLVFLGEEKGKRVNIERT
                     EEMVATGAKTIAAACPFCHSMFRDALGAPGMTPPALTDIAQLAAAPLQNANETNSGRL
                     "
     gene            6400..7383
                     /locus_tag="VLE22_22595"
     CDS             6400..7383
                     /locus_tag="VLE22_22595"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HSB17257.1"
                     /translation="MARIRYNEIFSGRFYLDLLVSGAAGFIGSHLCDRLLAEGHSVLG
                     LDNLLTGSRDNIAHLERHSRFEFRRQDVGKSFDANGDFDGVLHLASPASPKDYLGLPV
                     ETLEVSSCGTRNMLEVARRCNARFLLTSTSEAYGDPAEHPQSESYWGHVNPVGPRSCY
                     DEGKRFAEALTMAYHRTFGLRTSIARIFNTYGPRMKLKDGRVVPTFLDQALRRQPVTV
                     FGDGTQTRSFCYVSDMVEGLYRLFLSEERYPINLGNPAEMSIREFAEYICRLTGVPFK
                     AEYKPLPPDDPKKRRPDISKAKKSLGWEPHVSLEDGLRKTIEYFRPRMTFI"
     gene            complement(7376..7948)
                     /locus_tag="VLE22_22600"
     CDS             complement(7376..7948)
                     /locus_tag="VLE22_22600"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HSB17258.1"
                     /translation="MIERVSISEIKSLADYEIERSIGRPQIMALKDRRRVRVGDHLTF
                     LFENHDTVRYQIQEMIRIERTVRLEDIAHEVETYNELIPAENGLSASLFIEYETPEER
                     DVWLRELLGLEDHVWLAVGDLRPVRARFDGRQISGGRISSVQYVKFELTPEHAARFTD
                     GARIVVDHPKYRSEQALSTEQITELAGDLR"
     gene            complement(7945..9276)
                     /locus_tag="VLE22_22605"
     CDS             complement(7945..9276)
                     /locus_tag="VLE22_22605"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HSB17259.1"
                     /translation="MSIIHGAPSPGLSYNPNAPIYWDRPLLQQEVDRIFDICHGCRLC
                     FNLCPSFPELFQAVDRHDGNVRGLAPAEVNRVIDTCYQCKLCYVKCPYTPDDGHEFQL
                     DFPRLLLRMNAVRKKERGIDLRSRVLARPEMLGKVAGLTPALANWANRQPLMRRALEM
                     TLGIHRDKRLPEFHGETFEDWFHKRPVPAGDTSRAVLFVTCFANHNNPQLAKDTVEVF
                     AKNGISLSLPKQNCCGMPALESGDVDLAKRFAQSNVEALYPHVQTGKKILAINPTCSY
                     MFRKEYAELLGTPEAKAVAGATMDLCEFLFQLKQEGKFSRDFRSTPGKIAYHLPCHLK
                     AQNIGYRSRDVMRLIPGATIKLVDQCCGHDGTWAMKKEFFPLSMLAGKKAFDEMAAAE
                     PEVTATDCPLAAVHFEQALGTRPIHPIQVLARAYREDGFPKSIEPKEESAK"
     gene            complement(9364..9783)
                     /locus_tag="VLE22_22610"
     CDS             complement(9364..9783)
                     /locus_tag="VLE22_22610"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HSB17260.1"
                     /translation="MASLKGTKTHANLKDAFAGESQANRRYLYFAKQADVEGHPDIAG
                     LFRDTAEGETGHAHGHLDYLKSVGDPATDLPIGETVDNLKAAIAGETHEYTDMYPGMA
                     KAAREEGFAEIADWFETLAKAEKSHAGRFGKGLESIA"
     gene            complement(9853..10509)
                     /locus_tag="VLE22_22615"
     CDS             complement(9853..10509)
                     /locus_tag="VLE22_22615"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HSB17261.1"
                     /translation="MKITISKENYLKAIAEAESEGEVVIAATLARWLNVSAPAVTMAV
                     KRLRRDSLIDVGREGQLSLTPKGREIASHLLNRHHLIERMLTEIFGMEWYKVHEEAEQ
                     LEHAVSADFERRLVEKLGRDEACPHGNRVANDTPTERRLRGLLPLDEIEPACRVEVVS
                     VFERDRRLLEYLDELGVRPGVRITVVNRNYDDTMNLDTGARNVQLGPAAAGKVWVHPL
                     "
     gene            10562..11938
                     /locus_tag="VLE22_22620"
     CDS             10562..11938
                     /locus_tag="VLE22_22620"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated pathogenicity-related family
                     protein"
                     /protein_id="HSB17262.1"
                     /translation="MTAALPRSPILLLAWFSVLAAGERTALDRFVEAQDPAYKYQLVT
                     TLRGRDHTAYVLDMTSQSWRSSSEVDRVHWQHWLTIIKPDKVTLSTALLFIDGGANGR
                     RPPARADGMLLHFARSTGAIVAELRMVPNQPLQFPGDKMEKRIEDELIAYTWDKFLKG
                     ADELWPAQLPMTKSAVRAMDTVTAFCRSPQGGVVEVDRFVVSGASKRGWTTWLTAAVD
                     KRVVAIMPIVIDMLNLEPSFVHHWQVYGFWAPAVKDYVDMGIMRWMGKQEFRALMKLV
                     EPYEYRDRFTMPKYLVNAAGDQFFLPDSSQFYFDDLPGEKYLRYVPNTDHSLARSDAG
                     RSLLAFFESVLTGKPRPKFSWTLEKDGTIRLTVNDRPREVKLWHATNPSARDFRLETI
                     GRAWKARELRDQGGGVYVGSAPPPPNGWTAFFLEMTYPSGGKYPFKFTTQVRVVPDRL
                     PFPPPRAE"
     gene            complement(11949..12860)
                     /locus_tag="VLE22_22625"
     CDS             complement(11949..12860)
                     /locus_tag="VLE22_22625"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSB17263.1"
                     /translation="MTIEQLRLFRDIAHQRSLSRGAQLNGISQSAASQHLQEMERQLG
                     VPLLDRSTRPVELTECGRLYLEFCRDVLHLKEKLDSAMDRLKGRVEGTVRVASIYSVG
                     ISEMSWMEEEFSLRQPDAELRVEYLRPEKVYEAVLSDQVDLGLVSYPAASREIQVVRW
                     REERMVVAMAPSHPLAGLSLVKASDLNGEDFVGFDQDLPIHREVNRFLAEHGVEVNVV
                     LHFDNIQSMKEAIALGAGLSILPERVLRTDIEQKRLFARPLEAPGLVRPLGVIHRRRK
                     RFNAATQAFLELLMEKPAPNPTPIFAA"
     gene            12969..17567
                     /gene="gltB"
                     /locus_tag="VLE22_22630"
     CDS             12969..17567
                     /gene="gltB"
                     /locus_tag="VLE22_22630"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HSB17264.1"
                     /translation="MAARGYAHGLPPRQGLYDPGQERDACGIGFVANIQGVKSHDIIE
                     KGIQILLNLKHRGAAGCDKDTGDGAGLLIQIPHEFFARECSALGFALPPAGEYGAGIV
                     FLPVERHDRLHCEGIIERIIREEGLRVLGWRDTPVEADAIGRIARASQPYIQQIFVGR
                     APGMTQEALDRKLYVIRKRAEAEIAGSDIKEKGFFYIPSLCSRTIVYKGLLLAPQIAQ
                     FYEELSDPEVVSALCLVHQRFSTNTFPTWQLAHPFRYICHNGEINTLRGNVNWMYARQ
                     KVLASELFGEDLPKLFPIETPGGSDSGMFDNAAELLLLSGRSLPHAMAMLIPEAWDQD
                     PTMHPDKRAFYEYHASLMEPWDGPAAIAFTDGRVIGATLDRNGLRPARYLVTHDGLLI
                     MASETGVLPIRPEDIRHNGRLHPGRMLLADLEEGRIIPDQEIKERLWSRRPYAEWIRE
                     NQITLDKLPAPPRWHPTEFETILQRQRAFGYTDEDLKFILGPMAVKGEEPVGSMGTDT
                     PLACLSDKPQPLFHYFRQLFAQVTNPAVDPIREELVMSLTSYIGTERNILDETPLHCH
                     TLKLPHPILTNYDLEKLRRVSWGDFLATTLPMLYRVDGGEVELERAVTGLCRRASLAI
                     KNGYSLLILSDRGVDEEYAPIPSLLALSAVHHHLIREGTRTQVALIVESGEPREVMHL
                     SLLIGYGASAVNPYLAIETLEDMCRRGILGEVSFETALKNFKKAVNKGLLKVFSKMGI
                     STLQSYRGAQVFEAIGLNQELVDKYFTGTASRIEGVGLDVLAREAKEKHGHAFQPLTE
                     SDTELAVGGNYHYRVRGEYHLFNPMTVSKLQQAVRSETYATFKEYSDLVNRQSRQLCT
                     LRGLMDFRSAEHPISVEEVEPAREIVKRFATGAMSFGSISKEAHETLAIAMNRIGARS
                     NTGEGGEDEARFQRDPNGDWRRSAVKQVASGRFGVTANYLINADELQIKIAQGAKPGE
                     GGQLPGHKVDEVIARVRHSIPGVPLISPPPHHDIYSIEDLAQLIYDLKNVNPRARISV
                     KLVAEVGVGVVAAGVAKAHADVVLISGDSGGTGASPLTSIKHAGIPWELGLAETQQVL
                     VMNDLRSRIRVQTDGKLSTGRDVAIAALLGAEEFGFSTAPLVSMGCIMMRKCHLNTCP
                     VGVATQDPALRRKFSGTPENVINYFFFVAEELRELMAQMGFRTVDEMIGRVDMLKMRD
                     AIDHWKARGLDFAAIFHNPEVPQRVGRRCLVAQDHGLERALDHKLIELAHGAIENGTP
                     VELELPIKNVHRTVGAMLSGEVARRYGSQGLPDDTIRLRFSGSAGQSFGAFLAKGITF
                     VLEGDANDYTGKGLSGGRLVVFPPRTSTFVPEENILVGNVALYGATSGEAYFNGRAGE
                     RFAVRNSGATAVVEGVGDHGCEYMTKGVVVVIGRTGKNFAAGMTGGIAYVLDDTSEFS
                     SALCNTESVDLEAVPEGAEADSLQQIVARHFQATGSPRVGWVLENWEFVLPKFVKVFP
                     HEYKRVLGIPRASGQPRTILPLPGSGIQPPAQAVLGGD"
     gene            17579..19018
                     /locus_tag="VLE22_22635"
     CDS             17579..19018
                     /locus_tag="VLE22_22635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HSB17265.1"
                     /translation="MGKVTGFLEYARDVPQRRPASERVNDWFEIYLDFPEEKVRTQGA
                     RCMDCGVPFCHTGCPLNNIIPDWNDHVFQDRWRDAIRVLHSTNNFPEFTGRVCPAPCE
                     PACVLGINEPPVTIKVIEKTIIDHAFRLGWIHPEPPERRTGKKIAVIGSGPAGLAAAQ
                     QLNRAGHWVTVFEKADRIGGLLRYGIPDFKLEKRHIDRRLEQLAAEGVRFQTRAHVGH
                     NVPVEDLREEFDAILFAIGAEQPRDMNVPGRELKGIHFAMDFLPQQNKRVAGDVDIVA
                     DPSRFSEPVLATGKRVVIIGGGDTGADCLGTSHRQKALSVHQFEILPRPPEERSTSTP
                     WPLWPLQLRTESSHEEGGIRAWSVATTGFSGDEDGNVRKLHGVQVGPPPKFEALPGTE
                     FTLDADLILLAMGFLGPVRNGAIEALGLKLDQRGNVHTDENYMASLPGVFAAGDARRG
                     QSLVVWAIAEGRQAAKAIDRFLTGETRLP"
     gene            19169..20371
                     /locus_tag="VLE22_22640"
     CDS             19169..20371
                     /locus_tag="VLE22_22640"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSB17266.1"
                     /translation="MELERAYVLQNYSRLPIVIHRGKGCYVYDSDGKRYLDLITGIGV
                     NALGHAHPRILRVIREQASLLIHSSNLYYHEYQGPLAKRLCEISGLQRAFFCNSGTEA
                     MEGALKMIRSHGTKINPEKHEIISLDNSFHGRTLGAISVTGQAKYRKDFEPLLSGVKF
                     VPPNDSAALDAAFNDRTAGVVLEWIQGEGGVRPLPVEYVRHAKELAERHNALLVFDEI
                     QCGVGRPGTYFAYQLAEPVILPDVTVAAKPLACGLPLGAVMANDKAAASIGPGMHGTT
                     FGGGPLACRVALEFLDILQEQLTHIHHVGGYFRMELSELARHHSFIKDVRGFGLMIGV
                     ELEMPGKQIVTDAMEEGLLINCTQENVLRFLPPYIITEPEIDRAVKVLAKIFKRVKPP
                     AAGETAGA"
     gene            complement(20422..21501)
                     /locus_tag="VLE22_22645"
     CDS             complement(20422..21501)
                     /locus_tag="VLE22_22645"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HSB17267.1"
                     /translation="MRKANYYGLIMAGGRGTRFWPRSRRGNAKQVLRFFGEKSLVQDT
                     VDRLKPLIPPDRIWVLTNDYLRAEIVRQLPEVPRRQIIAEPAQRNTAPCIGLAAHILR
                     SIDSDAVMGVFPADHVVSRPARYRRLLTPAFREGDRGRIAVLGMQPRWPETGYGYIEF
                     PRGVRPGSLEALPVRQFREKPDIETAREFLAAGRFYWNAGMFFWRAEVYLDALRKFLP
                     KTAALLAALPPFSSRRFAGALAETYPLCENISVDYAVMERASGVTGLASDDVGWDDIG
                     SWNAFYELLPRDAEGNVTRGDTLLEASRGNYVESQGRLIALLGVENLVVVDTPDALLI
                     ADRSRSQQVGNIVKLLEKRKREELL"
     gene            complement(21514..22308)
                     /gene="lepB"
                     /locus_tag="VLE22_22650"
     CDS             complement(21514..22308)
                     /gene="lepB"
                     /locus_tag="VLE22_22650"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSB17268.1"
                     /translation="MANQDNLVMTDDASAGKHRPAGERPRGTIAEWTVTIILLLFGTT
                     TLVQAFVIPTGSMEDTLLIGDHLLVDKLAYAPAGPVSKYILPYEEIKRGDIIVFRYPM
                     DVTQTYVKRVVGIPGDRIHIRNKQVFRNGEKLNEPYTYFKTEYIDSYRDNFPSEPNTR
                     ISEAAQEMLEKHVVNSEIVVPPSSYFAMGDNRDSSFDSRYWGFVPRENIIGKPLIIYW
                     SYDAPTEQLASSTISLDHAIDLLQNFFTKTRWRRTFKLIRGHSFDN"
     gene            complement(22361..22657)
                     /locus_tag="VLE22_22655"
     CDS             complement(22361..22657)
                     /locus_tag="VLE22_22655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17269.1"
                     /translation="MGALPATEMPANNKPQGHRWYRILARVLLAVFCAEVGGFLLILP
                     WHETWSQNLFSGTSAQWYVVWTSPYFRGAVSGIGLVNLYISLVELSQLIRGPKT"
     gene            complement(22675..23358)
                     /locus_tag="VLE22_22660"
     CDS             complement(22675..23358)
                     /locus_tag="VLE22_22660"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSB17270.1"
                     /translation="MAGAATARIRCDSSHERMLRSRGYAMVAGIDEAGRGSLFGPVFA
                     AAVILSPDHPIRGLRDSKQLAAGKREVLAEQIRLFALAWAVASAEAEEIDQINILQAS
                     RLAMKRAVERLAVRPDFLLIDAVSVDLPIPQDGLVKGDCRCRSIAAASILAKVHRDAC
                     MDEWDAVYPQYGFRAGKGYPTPEHVQALEAFGPTPMHRFTFGPVRRFRFGDQMKLPLC
                     STEAAGQCR"
     gene            complement(23360..23707)
                     /gene="rplS"
                     /locus_tag="VLE22_22665"
     CDS             complement(23360..23707)
                     /gene="rplS"
                     /locus_tag="VLE22_22665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HSB17271.1"
                     /translation="MSDTLLKKFIVKKKRTDAPDFRPGDTIRVHVKIREGDKERLQAF
                     EGTVIARKNAGMGESITVRKISFGHGVERIFPVNAPVIDHIDVVRTGRVRRAKLYYLR
                     GLRGKAARLRERE"
     gene            complement(23704..24417)
                     /gene="trmD"
                     /locus_tag="VLE22_22670"
     CDS             complement(23704..24417)
                     /gene="trmD"
                     /locus_tag="VLE22_22670"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HSB17272.1"
                     /translation="MKFHVLTIFPEFFRGPLEHGVVARAAESGILSVQVHNLRDWTFD
                     RHKTVDDRPFGGGEGMVLKAEPIFLAVESILPEREAGRKVILLSAQGRLFDQPAAKRL
                     SGCEELLLICGRYEGVDERVAEHLADEEISIGDYVLSGGELAAAVVIDAVARLLPGVL
                     GNEDSAVFESFTGEDGQLGVLDCPHYTRPAEFRGWSAPEMLLSGNHEEIRRWRSRAAR
                     EKTARLRPDLLEKMKVAQS"
     gene            complement(24414..24977)
                     /gene="rimM"
                     /locus_tag="VLE22_22675"
     CDS             complement(24414..24977)
                     /gene="rimM"
                     /locus_tag="VLE22_22675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HSB17273.1"
                     /translation="MLSSPGTGTGGRWVTVAAIVNSRGNRGEVAAVPMSNRIERLARL
                     EKVYLFGADGSAIGGSPYEVESVWEHRGRVVFKFRGIDTISEAERLRGAEIRVPWTER
                     YQLPPGEYYHDDLVDCEVIDADSGSTVGMVANWQEFGGSGLLDVRTADGEEVLIPFAR
                     SICVNIDLEKRQITVRLPDGLKELNRR"
     gene            complement(24981..25220)
                     /locus_tag="VLE22_22680"
     CDS             complement(24981..25220)
                     /locus_tag="VLE22_22680"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HSB17274.1"
                     /translation="MAGMKELVEEIAKALVDIPDQVAVREVQGEQVTVLELKVAPSDL
                     GKVIGKQGRTARSIRTILGAAGMKLNRRFTLEILE"
     gene            complement(25290..25553)
                     /gene="rpsP"
                     /locus_tag="VLE22_22685"
     CDS             complement(25290..25553)
                     /gene="rpsP"
                     /locus_tag="VLE22_22685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HSB17275.1"
                     /translation="MLMIRLARFGAKKKPSYRVVVIEKDRARNGRSVEVVGYYKPQTK
                     PATVQLDQERIAHWLKSGAKPSDTVARLLKAQPEPTPATESVN"
     gene            complement(25593..26939)
                     /gene="ffh"
                     /locus_tag="VLE22_22690"
     CDS             complement(25593..26939)
                     /gene="ffh"
                     /locus_tag="VLE22_22690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HSB17276.1"
                     /translation="MFENLSDKLQRVFKNLRGEGKLTPENMEAALREIRVALLEADVH
                     FKVVKQFLEGVRQKAMGEEVLTALSPTQQVVKIVQEELVKLLGSHQSRLRFANEPPTM
                     ILIVGLQGSGKTTTTGKLARWLSRNGHLPLMVSVDVYRPAARDQLAVIARDLRLPIYE
                     GAPEEKAPAELAKSARREAIQTGRDVVLVDTAGRLHIDEQLMAELQELKQLLSPSEIL
                     FVADAMTGQDAVKSADEFHKRLGITGAILTKMDGDARGGGALSIRHVTGQPLKFVGVG
                     EKFDALEPFHPDRVASRILGMGDVLSFIEKVEESIDQKNAEEMQRKLIDNEFTLEDFR
                     DQLKQLKKLGPLDSILSMMPGVGPLKDLKNVKVDEKEIVRVVAIIDSMTPKERANHMI
                     INGTRRRRIARGSGTTVQEVNQLLKQYAQARKMMKTFSGGFMGKRLGKLKLPAFGG"
     gene            complement(27052..27720)
                     /locus_tag="VLE22_22695"
     CDS             complement(27052..27720)
                     /locus_tag="VLE22_22695"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HSB17277.1"
                     /translation="MSFSPLNEVEVRVLGALIEKDLSTPEYYPLSLNALVNACNQKSN
                     RHPVVSYDEATVSQAADSLRERRLALVTTGGEHRVPKYGHRISETLNLGNRELAILGE
                     LMLRGPQTPGELKSRAQRLHAFDDLESVESCLRRLIEWQPEPVVVQLPRQAGMREVRY
                     AHLLGGEVAVPEVEAASGSEARLGARSEIEERITRLETEVESLRSGLEDLSGRLESFV
                     RQFQ"
     gene            complement(27717..28688)
                     /gene="pdxA"
                     /locus_tag="VLE22_22700"
     CDS             complement(27717..28688)
                     /gene="pdxA"
                     /locus_tag="VLE22_22700"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HSB17278.1"
                     /translation="MNKKPTVALTIGDPAGVGPEVVVKALADEGVTCLARWIVVGDAW
                     VIRRAEALTGLGMPAPEAARREDLRQLEASQCETGRLSGACGRAALGYVRRATELCLT
                     GIADAMVTAPLNKEAVTMAGVRFSGHTEFIEELSGARESRMLLASDRLRVVHVSTHVP
                     LRKACDLDPARILRTIELGEEAMRLLGFRNPRIAVCGLNPHAGEHGLFGDEDARFIAP
                     AIDVAMQRGIQCIGPAPADTVFLRAVRGEYDLVVAMYHDQGHIPMKLLDFEHTVNVSL
                     GLPIIRTSVDHGTAFDIAGKNLADPGSMKAAMRLAATMAAGKKGNRL"
     gene            complement(28685..29752)
                     /locus_tag="VLE22_22705"
     CDS             complement(28685..29752)
                     /locus_tag="VLE22_22705"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HSB17279.1"
                     /translation="MNFEDNVPWGVVADDLTGACDAGVQFAQRGLRTIVWLNAEAAEI
                     EAADVTVVTTNSRRDTAETAAVKTRHACRVLRAAGARVLYKKIDSTLLGNLGPEIETV
                     RIEGEFPLAFVTPAFPEMGRTVMNGLLHVRDFGATVDVAERLREQGLETLVQRTPGQL
                     GGLREEAGTTVVLDATSRADLAAIARAAWVMRPRPLLAGSAGLAAELAACLTGRATDS
                     GVTCVPNLEADRRRIALIVGSTNPVTVRQAEYAEAHLRGRYPIMRISMEQSPSRVADL
                     SAILKDARGIVLTGGDTALMVCGMLEARGIRLEREILPGIPWGRLIGGSAGGTVVATK
                     AGGFGEIDALVRVADFLAATE"
     gene            complement(29771..30742)
                     /locus_tag="VLE22_22710"
     CDS             complement(29771..30742)
                     /locus_tag="VLE22_22710"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSB17280.1"
                     /translation="MSSLPLFSLDRLSLALVAAVRIVMILVVAFILVRMINKWVPKLK
                     QRIVDSMLRHGAGSHGELEKQAATLGGIFRRTLSVLIWTGALVMSLKEAGFDIGPILA
                     GAGVVGLAVGFGAQNLVRDTISGLFMLIENQVRVNDVAVINGTSGLVEEINLRTTVLR
                     GADGTVHVFPNGVITSLSNMTREYSYYVLDIGVAYKEDTDNVMAVLKDIAAQMMKEDR
                     YNVLILEPLEVLGVDRFAESAVIIKARIKTLPIQQWTVGREMNRRIKKRFDELGIEIP
                     FPQRTLYLAGAPGQADPEQLKTLVREVLDERAAPARSPGDHGTSPAT"
BASE COUNT         5861 a         9763 c         9270 g         6187 t
ORIGIN      
        1 cttgatccac cagccgtacc caattttgaa gttgttcacg ccgctcggat cgtctcccgt
       61 ccggttgggt ttccagaggt cgttgatgat cttctggcct gttggatcga tcatctgagc
      121 cgggatcctg ttgtttgtga agggagtccg ggtcaccgtc gaggtggcgg gatcgaactg
      181 tgtggagaac ggatcgtaga tcatcctcaa gttgccgtca ggcgtgaacg ttttggagaa
      241 atcgccggtg cgctcgaggt ccgtcggaac tgtggaaacg ttcgaggacg gctgggtggc
      301 cttccaccgc tcgtagctga aaaagttgaa cagcttgttc ttcttgatcg ggttgccgag
      361 ggtgccgccc cagatgttgt tcttcacaac gctctcggtt cgggtgatcg gatccaccac
      421 ggcgttgaag acggggttgc ggccgaacca ataggccgaa ccgtggaaat cgttggtgcc
      481 ggacttcatc gagaggttga gcactccgcc ggcgctgaaa ccatactccg cgtctacggc
      541 gttctgctgt accaccactt cctgcaccgc gtccatcggc gaattgtagg aaccgcgcgc
      601 ggccacgccc gtaggcacgc catcgagcaa ctggtcgttc ttgccgcccg tcgatccgcc
      661 tacatccagt ccggagttgg accacatgta gaacgggttg cgatgcgaga catcccagta
      721 ctggttcacc accgccggat tgaggagggc cagcgtgaag ggattccgtg ccagcacggg
      781 gaggtcctgc agcatggtgc cctgaaccgc ggtggtcatg gtgctggtgt tgaactgcac
      841 cgcggtgacg gcctcgctga ccgtgaccgc ctcggtaacg cctcctactg tgagcggggc
      901 gttgacggtc acgtcgccag ctgtcagcac ggtgatgttt tcctgcacaa acttgctgaa
      961 accggaggct tcaacggtca cagagtaact gcccggcgtg acgaagtcga agcggtagct
     1021 gccggtcgtg tcagtttctt ttgcgctttc gatgccagtg ttgacgtttc tcaatgttac
     1081 tttggccccg acgatggctg cctgtgtcgg gtcagtcacc gtgccctgca cgcggccacg
     1141 ggactcctgg ccgagtgcaa agctcgtgaa aacaatcagc aaggcgaagc aaagcacggt
     1201 cattccccaa cgggaaatgc gttgcataca accccctttt ctgttgagac actgcgaaag
     1261 tagttggata gaaacctcaa gctttggcga ccccattctc ggaccttagc ctgacgtcat
     1321 gcaacagtcc taccccacca ggtcgtagac ccaaaattgt cctgttgcct tggccaatta
     1381 tgtaccccgt tacacaggct gtcaattaga gtttccttaa tgaatgtata tttatcgtga
     1441 tcatatttcc gcattgacag tacgttttct tgtatggcag tgcaggggta agtaccgtgg
     1501 cgatccggtg ctagcgaacc ggctcggctg gctgttcctt cgccttcagt gccttgtaca
     1561 ttccgagggt cttacggaaa tcgctgtcgc ggtccaggtc gcggtaggcg ccggcaagag
     1621 tgaagaacac ttcgctgtca ccgggactca gcttcgcggc agcctcaaga tgcggcaagg
     1681 cttcggctgg ctttccctgt ttcagaagga gcttgccaag ttcatagcgg gcgccgggga
     1741 gtgacgcgtc aagcgcaagc gccttcttga gcagggactg cgcgcgtgct tcggcagcgg
     1801 cgtcgggttt ggcggtgggt tgtaccaaca tccggccgta agcctcatag aaacgggcgt
     1861 tgcgagggta ccgggcgatc cccttttcga aagttgcgga tgcctgagag atcagccaac
     1921 cggcagcctg cgcttggcct agcaggagca gcgcctcatc gtcatccggg gcgagctcca
     1981 cggcgcgcgt caaagagccc accgcctcgt ggaggctcct gatgtagcct tccgcctggc
     2041 ctttgcgctt ataggccagg gcggacttcg gcattctctc gacggtccgt agggtgaggt
     2101 cgtaggcctc tgagtaacgt ctctgctcta tcaggagcgc cgcgagttcg ccgtagccgg
     2161 tatggttcga gggttcgatc tcgatggcct gcatcatcgt gcgcacggcc tcggccggct
     2221 gtacctgccg ttggtacgcc catgccagaa ggcggtaaag acgggggtcc cgggatccgc
     2281 cggcgatggt cgcaaggagc gtcgaccggc actcttcgaa gcggccggcg cggtaataac
     2341 agcgcgccag tgaaaggatt gcatcgggcg tctttggaac gaggcccgga agagactcga
     2401 gcagcttcag cgccgcttcg tcttcgccga gactctctag aacacttccg agcagcagag
     2461 acgcctgtgg atgcccgggt ttcgccttga gaatccgctc gagatgcttc ctggagtccg
     2521 gactccggcc aagccggaac tgattcagcg ccaggttgta gcgagtgccg gcgtccgagg
     2581 gatccagttt cagcgccgcc tcgaagtgga cgttcgcctc ttccagtttc tgcccgccgg
     2641 tcagagccat tccgaggaga gaatgcgcga cggcattgcc cggagccgcg cgcacggcga
     2701 ttcgaaattc cgcttcggcc tgctgtatcc ggccttcgcg caaaagcgaa ctcccctttc
     2761 ttaggtgctc ctgcggagac acggactttg ccggagggga agcgacctgc aacgcaagag
     2821 tcagaacgcc agcgatcatg ctttcgcccc agggcggcgt gaacggttcc ttactctcca
     2881 ggctagtccg gaggctcgct aacctgcaag acctggttga ccgggatctc cgcaagtacc
     2941 tgtttgactc cgctcggcca gcggatgtcg atgtcggtag ccttggccac gccgcctagc
     3001 ccgaagtgga cgccaaagtt gctcgacgat gcgtacccgc cgttgacggt gacgtggttg
     3061 tgctgagttc caatgcgaac gctcgcgccg atcccatcct tgttgcttcg cgcgccgtgc
     3121 agccgcagaa caagccacga agcaaccgaa gggccggtgt tttcccagag ctcaggcggt
     3181 ccaccaagca cagccacgac gacatcaagc ctgccgtcgt tgttgaagtc cgccgcggct
     3241 gcgccacggt gagcgcgggg agtctggaaa tccgggccgg cctcaccgga aacgtcacga
     3301 aaagacccat tgccggtgtt gagaaaaatg cagttcggga ccttgtattt cgtgggctcg
     3361 aagtattcga cgttgtcgac gacatgggcg ccggaaacga acagatcctt ccggccgtcg
     3421 ttattaaaat cgtacaagag gggactccag ccgctgcggt cgatgctgag ccgggacagg
     3481 gagctacgat cggtccagtc gcgaaagcgg ctgcggccca gattccggaa aagagggaac
     3541 cactcgccgg caagcgcggc gaccacgatg tcgggaaggc cgtcgttgtc gtagtcgcgg
     3601 aagtctgccc ccattgcgga gatggggcgg gcgtggtcgg ccatggccac gccggactcc
     3661 agcgccacct cctcgaactt gccgtttccc agattgtgga acagaaagtt ggggatcttg
     3721 tcattggtga cgaaaacatc cacgaagccg tccaagtcgt agtcggccaa agcgacgctc
     3781 attcccttcc caacgtggtg ggcgatacca gacttctcgg acacgtcctc aaaggtgccg
     3841 tcgccacggt tccggtaaag cgtgttcggc aaaccccgga agtgtttcgg gtgacagtag
     3901 gcgcggacat tcttgacgga gtcgccgcag aacggagcct tgtcgggaat ccagtccagg
     3961 taattcacga cgaacagatc gaggcggccg tcgttatcgt agtcaagcca gcctccggcg
     4021 atggaccaca ggtcgctctt gatgccggct ttggcggtga cctcctcgaa cctgccgtta
     4081 ccgaggttgt ggtagaggtg gttagatcgt acccccgcga cgaacaggtc cgtatgaccg
     4141 tcgttgtcaa agtccgcggc ggaggctcca atggagtagc cggaaccagc gaggccagcc
     4201 tcagaagtta cgtccttgaa cttcatccct cccaagttcc ggaacagccg gttgtgaaac
     4261 ttgggatcct tcttctccag ggaagggacg gacgcgccgt tcgtgaagaa gatgtccatg
     4321 agcccgtcgc cgtcgtagtc gaaaacggcg agcccgccgg ccatcgtctc gatgatctgt
     4381 ttctcggggg tagggttgtt ttcgaggaca aaccgcaggc ccgcggcttc cgcaacgttc
     4441 cggaatcgga tgggcggcgg ttgtgcagac gaaaccgaag tggccagaca gagggaccag
     4501 aagagcctac aaacgcccgg aattggtttc gtttgcattt tggagtggcg cagccgccag
     4561 ttgcgcgatg tcagtcagcg ctgggggcgt cattcccggc gcgccgaggg catcgcggaa
     4621 catgctgtgg cagaagggac aggcggcggc gatcgttttc gccccagtcg ccaccatttc
     4681 ctcggttcgc tctatgttta cccgtttccc tttttcctca ccaaggaaca caagcccgcc
     4741 gccggcgcca cagcagaaag aactgctgcg cgtccgccgc ggctcaaccg tctctccgta
     4801 aagccggatc acatcgcgag gctcttcgta agtgccgcgg tagcgtccga ggtaacacgg
     4861 gtcgtggaag acaacggtat tacggggacc actcgcggcc gggaggcggt cccggtggcg
     4921 ggcgaggaac tcgctgtggt gctcaatttc aatgtccgcg ccgaattcct tccagtcctc
     4981 cgatatcgtg cggacacagt gcgggcaaat cgaaacaagc ctgcgggctt tggaccggcc
     5041 gatttcttct atgttcgctt cggccagttg ggagaacagc aggtcgtttc cgagcctgcg
     5101 cgcggagtca ccggtacagc gctctttctt taggacgccg aaggtgacct taagataccg
     5161 cagcacggtg accatcgcca gaacgatctc cctcccctgc ggatcgtagg agcccatgca
     5221 ccccaaccaa aggcagtatt cttgggcgcc gtcgtacagc ggcagcccat gcttttcgat
     5281 gaacttgtcc cgctcggacg acgcaaaacc gaggggattt ccatatcgct ccaggttcag
     5341 gaacagcttg ttgccgtgat cgtcatccca tttgccggta ttgacggcgc cgcggcgcag
     5401 gccgacgatg accggcaggt gttggattcc aaccgggcac tgatactcgc aagcgccaca
     5461 agttgtgcac tgaaaggccg cttcttgcga aagatgcctt ccgatcaaag gagcctccgc
     5521 cacagctccg ttttcattca aataccggcg cactccgaga atgatctcct tcggattcag
     5581 gactttgcct gtgttgtagg ccgggcagtg ttctgtgcag cgcccgcact cgacgcaggt
     5641 gtaggcctga agagtgacaa tccgcgtgag atcccggccc gtatcgagac cgaagtcttc
     5701 atcgcccgcg agaggcggaa tacggctgaa tgagggccgc ttcaggaaca ccgtcaccgg
     5761 gctaagcagc aagtgcagat gcttcgtatg cgggatcagc gggagaaata ccaacagtgc
     5821 cagcgtatgc gcccaccagt tgattcgtgc cgccggactc ccctccgtga cgaagaatgt
     5881 cgtcaggtag gtcgccatca ggaagaagat caggaaggcg accagacccg attccggaga
     5941 aatctccccg agccatttcg gcctcgcaac gaaccgccgg attgcgagtc cggcgatcga
     6001 cagaccggcg gcgaccgcga acacggctgc tacgatgaag tacagctgcc cgaatccact
     6061 atcgcgagga atgagttcca gccccagccc ttccacgagg tgattgatcg tcaccagcgc
     6121 gaatgcgcag aatccccaaa acacaaatgc gtgcgctatg cccggcagcg ggcgatcccg
     6181 gatcacgcgc tcttggagca tcacctgccc gaagaaagcg cgcatgcgcg gcaagagcgg
     6241 ccgcagttgg aagtccgggt ctctccggga acgccgaatg acggcggcga ctcttccgaa
     6301 ccgccgccaa aacgcgagtg cggaagcggt tagcagcgcc aggaagagca agatttccca
     6361 cggagaggac ccgggcatca tgcctgctct gaatgtaaca tggcgcgaat tcgctacaat
     6421 gagatcttct ccgggaggtt ttatttggat cttcttgttt cgggagcagc cgggttcatc
     6481 ggctcgcacc tctgtgatcg cctgctcgcc gaagggcatt ccgtgctcgg cctcgacaat
     6541 ctgctcaccg gctcccgcga caacatcgct cacctcgagc ggcattcgcg gttcgagttc
     6601 cgccggcagg acgtcggcaa gtcattcgat gcaaacggcg actttgacgg cgtgctccat
     6661 ctcgcttctc ccgcgagccc gaaggattac ctcggactcc ccgtcgagac gttggaggtt
     6721 agttcgtgcg gaaccaggaa catgctggag gtcgcgcgcc ggtgcaacgc acgcttcctg
     6781 ctgacgtcga catccgaggc ttacggcgat ccggcggagc atccgcagtc ggagtcctat
     6841 tggggtcacg tcaaccccgt cggcccccgc tcatgctacg acgaaggcaa gcgctttgcc
     6901 gaggcgctga cgatggcgta ccaccgcaca ttcgggctgc ggaccagcat cgcccgtatc
     6961 tttaatacct acgggccgcg gatgaagttg aaggacgggc gcgtcgttcc aacgttcctg
     7021 gaccaggcct tgcgccggca gccggtcacc gttttcggtg atggtacgca aacccgcagc
     7081 ttctgctatg tgagtgacat ggtcgaaggg ctgtaccggc tctttctgtc cgaggaacgc
     7141 tatcctatca acctcggtaa tccagcggag atgtcgattc gggagtttgc cgagtacatc
     7201 tgccggctta cgggtgtccc gttcaaggct gagtataaac cgctgcctcc cgacgacccg
     7261 aagaaaagac ggccggacat ctccaaggca aaaaagtcgc ttggctggga gccgcacgtg
     7321 tcgcttgagg acggactccg caagacgatc gagtattttc ggccgcggat gacgtttatc
     7381 tgaggtcccc tgccagttcg gtgatctgtt cggtactgag cgcctgctcg ctccggtact
     7441 tcgggtgatc cacaacgatt ctcgctccgt cggtgaaccg tgcggcgtgt tccggcgtaa
     7501 gttcgaattt cacgtactgc acggaactga tccggccgcc ggagatctgg cgaccgtcga
     7561 acctggcccg caccggacgc aggtccccca cggccagcca gacgtggtcc tccaggccga
     7621 gcaactctct tagccacaca tcccgctcct ccggggtctc gtattcgata aacaaactgg
     7681 cgctcaggcc gttttccgcc ggaatgagtt cgttgtacgt ttcgacttcg tgagcgatgt
     7741 cctccaaccg gacagtccgc tcgatgcgaa tcatctcctg gatctgatac cgcacggtat
     7801 cgtgattctc gaacaagaac gtgagatgat ccccgacccg gacacgacgc ctgtccttga
     7861 gggccatgat ttgcgggcgc ccgatgcttc gctctatttc gtagtcggcg aggcttttga
     7921 tctccgaaat cgaaacacgc tcgatcactt cgctgattcc tccttgggct cgatgctctt
     7981 cggaaaaccg tcttcgcggt aagcgcgggc cagcacttga atcgggtgaa tgggtctggt
     8041 gcccagcgcc tgctcgaagt ggaccgccgc cagcgggcag tcggtggcag tgacctcggg
     8101 ctccgcggcg gccatctcat cgaacgcctt cttccccgcg agcatcgaga gtggaaagaa
     8161 ctccttcttc atcgcccacg tgccatcgtg accgcaacac tggtccacca gtttgatcgt
     8221 ggcgcccggg atcaacctca tcacgtcccg agagcggtac ccgatgttct gggccttgag
     8281 gtggcaggga aggtggtagg cgatcttgcc gggcgtggat cggaagtccc ggctgaactt
     8341 gccttcctgc ttgagttgaa acaggaattc gcacaagtcc atcgtcgccc cggcgacggc
     8401 cttcgcctcc ggagtgccca gtagttcggc atactccttg cgaaacatgt acgagcaggt
     8461 cgggttgatc gccaggatct tcttgccggt ttgcacgtga gggtagagcg cctcgacgtt
     8521 gctctgcgcg aagcgtttgg cgaggtcgac gtccccggac tccagcgcag gcattccgca
     8581 acagttctgt ttcggcagcg aaagcgagat gccgttcttc gcaaagactt cgacggtgtc
     8641 cttcgccagt tgcgggttgt tgtggttcgc aaagcaggtg acgaagagca cggcgcgtga
     8701 cgtgtctccc gccggcacgg gccgcttatg gaaccaatcc tcgaacgttt ctccgtggaa
     8761 ctcgggcagt cgcttgtcac ggtgaatgcc gagagtcatt tcgagcgcgc gccgcatcag
     8821 cggctgccgg ttggcccagt ttgccagggc gggcgtgagt ccggcgacct tgccgagcat
     8881 ctccggccgg gcgaggaccc gggaacgcag atcgatgccg cgctccttct ttcggactgc
     8941 gttcatccgt agcaagagcc gcggaaaatc aagctggaac tcgtggccat cgtcgggtgt
     9001 gtacggacac ttcacgtagc acagcttgca ttggtagcag gtgtcgatga cacggttgac
     9061 ctcggccgga gccagccccc gcacattacc gtcatggcgg tccactgcct gaaacaactc
     9121 ggggaacgac gggcagagat tgaaacagag gcggcaaccg tggcagatgt cgaatatccg
     9181 gtcgacttcc tgttgcagca acggcctgtc ccagtaaatg ggcgcattgg ggttgtagct
     9241 caaccccgga gagggggcgc catgaatgat ggacatgggt tggagaccga aacggggaca
     9301 ggaggcacac gacccgaatt gactacaacc ggatcggcaa aacccgtccc cgcttcctta
     9361 agttcaggcg atgctctcca ggcccttgcc gaagcggcct gcgtgtgatt tctcagcctt
     9421 tgccaaggtt tcaaaccagt ccgcaatctc ggcgaatcct tcttcgcgcg cggccttggc
     9481 cattccgggg tacatgtccg tgtactcgtg ggtctcgccg gcgatcgcgg ccttgagatt
     9541 gtcgacggtt tcgccgatgg gcagatccgt ggcgggatct ccaacactct tcaggtagtc
     9601 gagatgaccg tgggcgtgcc cggtctcgcc ctctgccgtg tcacggaata aacctgcgat
     9661 gtccggatgc ccctcgacgt cggcctgctt cgcaaagtac agatagcgcc ggttcgcttg
     9721 agactctccg gcgaaagcgt ccttcagatt cgcgtgcgtc tttgttcctt ttagggatgc
     9781 catttgagtt tcctccgttc gaatcttttg tcaaataaga actattatac gttgtcaacc
     9841 cgggggatct ggtcagagcg ggtggaccca aacttttccc gccgcggccg gaccaagttg
     9901 aacatttcgc gctcccgtgt ccagattcat cgtgtcgtcg tagttgcggt tcacgacggt
     9961 gatgcgcacg ccgggccgga ctccgagctc gtctaaatac tcgagcagac gcctgtctcg
    10021 ctcgaacacg ctgactacct caacccggca cgcgggttcg atctcgtcga gaggaagcaa
    10081 gcctcggagc ctccgctccg ttggcgtatc gttggcgaca cggttaccgt ggggacacgc
    10141 ctcgtccctc cccaacttct caaccagcct ccgctcgaaa tccgcggaaa cggcgtgctc
    10201 gagctgctcc gcctcttcgt gaaccttgta ccactccatc ccgaagatct cggtgagcat
    10261 gcgctcgatc aggtggtgtc ggttcaagag atgactggcg atctcacggc ccttcggagt
    10321 caaagaaagc tggccttccc gcccgacatc gatcaacgaa tcccggcgaa gccgcttgac
    10381 cgccatcgtg acggcaggag cggagacgtt gagccagcgg gccagagtcg ccgcgatcac
    10441 gacttcgccc tcggactcag cctcggcaat tgccttgagg tagttttcct tggaaatcgt
    10501 gatcttcacg ccaatccaga ttgtagcgcg ggaagaacgc ctgttatcct ctgggtttgc
    10561 gatgactgcc gcacttccga gatccccaat tctactgctg gcctggttca gcgtattagc
    10621 ggccggggag cgcactgcgc ttgaccgttt tgtcgaggca caggacccag cgtacaaata
    10681 tcagctagtc acaacgctgc gcggcaggga ccacaccgcg tacgttctcg acatgacctc
    10741 gcagtcctgg agaagctctt ccgaggtgga ccgtgttcac tggcagcact ggctcacgat
    10801 catcaagccc gataaggtga cattgtcgac agccttgctg ttcatcgacg gtggcgccaa
    10861 cgggcgaagg ccgcctgcgc gcgcggacgg aatgttgctc cacttcgcca ggtccaccgg
    10921 cgcaatcgtg gctgagttga ggatggtacc gaaccagccg ctgcaattcc cgggcgacaa
    10981 gatggagaag cgcatcgagg acgaactgat cgcgtacaca tgggacaagt ttctgaaggg
    11041 cgccgatgag ctttggcccg cacaactccc tatgaccaag agcgccgtcc gtgcaatgga
    11101 tacggtgacc gcattctgcc ggagtcccca gggcggcgtc gtcgaggtgg accgctttgt
    11161 ggtttcgggt gcatccaagc ggggctggac aacctggctg accgcggcag tcgacaaacg
    11221 cgtggtcgcc atcatgccga tcgtaatcga tatgttgaac ctggagccct ccttcgtcca
    11281 ccactggcaa gtctacggat tctgggcgcc cgcggtgaag gattacgtcg acatggggat
    11341 catgcggtgg atgggcaagc aggagttcag ggctttgatg aagctcgtgg agccatacga
    11401 gtaccgcgat cggttcacga tgccgaagta tctggtcaac gccgcaggcg accagttctt
    11461 cctgcccgac tcttcgcagt tctacttcga cgacctgccc ggcgagaagt acctccgcta
    11521 cgttcccaac acggaccact cccttgcgcg atcggacgcc ggccggagtc tgctggcgtt
    11581 cttcgaatca gtcctcactg gtaagcccag gccgaagttc tcctggacgc tcgaaaagga
    11641 cggaacaatc cgcttgacgg tgaatgaccg gcctcgcgaa gtgaagctct ggcacgcgac
    11701 caatccaagc gcccgcgatt tccgcctgga aacaattgga cgtgcctgga aggcacgtga
    11761 acttcgtgac cagggcggag gagtctatgt cggctcggcg cctccgccgc cgaatggctg
    11821 gaccgccttc ttcctggaga tgacctaccc cagcggcggc aagtacccgt tcaagtttac
    11881 gacccaagtg cgggtagttc cggacaggct gcccttcccg ccgccaagag cggaatgaag
    11941 gcggtgtctc aagccgcgaa aatcggtgtc gggttgggag cgggcttttc catcagcagt
    12001 tcgaggaacg cctgagtggc cgcgttgaac cgcttccgcc gccggtgaat cacacccagc
    12061 ggccggacga gaccgggagc ttcgagcggt ctcgcgaaca ggcgcttctg ctcaatgtcg
    12121 gtgcgcagga ctcgctcagg caggatactc aggcccgcac ccagcgcgat cgcctctttc
    12181 atcgactgaa tgttgtcgaa gtgcagcaca acgttgacct cgacgccgtg ctccgccaga
    12241 aatcgattga cttcgcgatg gattgggagg tcttggtcga aacccacgaa atcttcgcca
    12301 ttcaggtccg acgccttgac gagactcagg cccgcgagcg ggtgactcgg cgccatggcc
    12361 accaccatcc gttcctcgcg ccacctcacc acctggatct ccctgctggc cgcggggtag
    12421 ctcaccagtc ccaggtcgac ctgatcggac agcaccgctt cgtaaacctt ctccggacgc
    12481 agatactcaa cacgcagttc ggcatccggc tgccgcaagg agaactcttc ctccatccac
    12541 gacatctccg agattcctac cgagtagatc gaggccacgc ggacggtacc ttccacccgc
    12601 cctttgaggc ggtccatagc tgaatccagt ttctctttga ggtgcaatac gtcgcggcag
    12661 aactccaggt acagccggcc gcactcggtc agctcaactg gacgcgtgct gcggtcaagc
    12721 agagggactc cgagttgtct ttccatctcc tgaagatgct ggctcgccgc ggattggctg
    12781 attccattca actgtgcgcc ccggctcagg ctgcgctggt gagcgatgtc cctgaaaagc
    12841 cgaagctgtt cgatcgtcac gatttcatcc tgccataagc tatgctaatt ttgcaagcat
    12901 ttaatttttg gttgacttat gttgtggcta tgctatactg gcctcaactt atctcgggga
    12961 ggatcacgat ggctgcccgc ggctacgcac acggtttacc tccacggcag ggactctacg
    13021 atcccggtca ggagcgggac gcttgtggga tcggcttcgt cgccaacatt cagggcgtga
    13081 agtcgcacga catcatcgag aagggtatcc agattctcct caacttgaag catcgaggag
    13141 cggccggttg tgacaaagac acgggggacg gtgctggtct tctgattcag atccctcatg
    13201 agtttttcgc cagggagtgc tcggccctgg gttttgcact gccgccggcg ggagaatacg
    13261 gcgccggcat cgtattcctg ccggtggaac gccacgaccg gctgcactgt gagggaatta
    13321 tcgagcggat catccgcgaa gagggtttgc gggtcctggg ctggcgggac acgccggtcg
    13381 aggcggacgc gatcggccgg atcgcccgcg cctcacagcc gtacattcag cagatcttcg
    13441 tcggacgtgc gccgggcatg acgcaggagg ccctggaccg caagctttat gtcatccgca
    13501 aacgggccga ggcggaaatc gccggctcgg acatcaaaga aaagggcttc ttctacatcc
    13561 cgtcgctgtg ctcgcgtact atcgtctaca aaggcctgct tctggcgccg cagatcgctc
    13621 aattctatga ggaactgtcc gaccccgaag tagtcagcgc gctttgcctc gtgcatcagc
    13681 gcttctcgac caacactttc cccacttggc agcttgccca cccgttccgg tacatctgcc
    13741 ataacggcga gatcaacacc cttcgcggca acgtgaactg gatgtacgcc cggcagaagg
    13801 tgcttgcttc ggaattgttc ggcgaggatc tcccgaaact cttccccatc gagaccccgg
    13861 gcggcagcga ctcggggatg ttcgataacg cggcggagtt gctcctcctt tcgggccgga
    13921 gtttgccgca cgcgatggcg atgctgattc cggaagcgtg ggaccaagat cccaccatgc
    13981 atccggacaa gcgcgccttc tatgagtacc acgcgtcgct gatggagcct tgggacggcc
    14041 cggccgccat cgccttcacc gacgggcgag tcatcggggc cacgctcgac cggaacgggc
    14101 tccgcccggc cagatatctg gttacccatg acggcctgct gattatggcc tccgaaaccg
    14161 gcgtgctgcc gattcgcccc gaagacattc gccacaacgg caggcttcac cccggccgga
    14221 tgctgcttgc ggatctcgag gaaggccgca tcatcccgga ccaggagatc aaggagcggc
    14281 tttggtcgcg gcggccgtac gccgagtgga tccgggagaa tcaaatcacg ctcgacaagc
    14341 tgccggcgcc tccgcgctgg cacccaacgg agttcgaaac catcctccag cggcaacgcg
    14401 ccttcggata caccgacgaa gacctgaagt tcatcctggg accgatggcg gtcaaaggcg
    14461 aggaaccagt cggctccatg gggaccgata cgccccttgc ctgcctctcc gacaagccgc
    14521 agccgctgtt ccactacttc cgccagctct tcgcgcaggt caccaatccc gcggtcgacc
    14581 cgatccgcga agagctggtg atgtcgctga cgagctacat cgggaccgag cgcaacatcc
    14641 tcgacgaaac accactccac tgtcacaccc tcaagctccc gcacccgatc ctgacgaact
    14701 acgacctcga aaagctccgc cgggtgtcgt ggggcgactt tctggcgaca accctgccaa
    14761 tgctctaccg cgtcgatggc ggagaagtgg aactcgaacg cgccgtcacg ggtctctgcc
    14821 gccgcgcctc gctcgccatc aagaacggct acagcctgtt gatcctgtcc gaccgcggcg
    14881 tcgacgagga gtacgcgccg atcccgagtc ttctggcgct ttcagccgtg caccatcacc
    14941 tcattcgcga gggaacgcgg acccaggtgg cgctcatcgt ggagtcgggc gagccgcgcg
    15001 aggtgatgca cctctccctc ctcatcggct atggtgccag cgcggtgaat ccctatctcg
    15061 caatcgaaac tctcgaggac atgtgccggc ggggaatcct cggcgaggtc tcgtttgaaa
    15121 cggcgctaaa gaacttcaag aaggctgtca acaagggact gctgaaggtt ttctcgaaga
    15181 tgggcatttc gacgctccag agctatcgcg gcgcccaggt cttcgaggcg atcggcctga
    15241 accaggagct ggtggacaag tacttcaccg gcacggcgtc ccggatcgaa ggcgtcggtc
    15301 tggacgtcct cgcccgcgag gcgaaggaga aacatggcca cgcgttccag ccgctgactg
    15361 agtccgacac cgagcttgcc gtgggcggca actatcacta ccgggtgcgg ggggagtacc
    15421 acctgttcaa cccgatgact gtcagcaagc ttcagcaggc ggttcgctcc gaaacgtacg
    15481 cgaccttcaa ggagtattcc gatctcgtca accggcaaag ccgccaactc tgcacccttc
    15541 gcggtctgat ggatttccgg tcggccgagc atccgatctc tgtcgaggag gttgaaccgg
    15601 cgcgagagat cgtgaagcgc ttcgccaccg gagccatgtc gttcggctcc atcagcaaag
    15661 aggcgcacga gacgctggcg atcgccatga accgcatcgg agcgcggtcc aacacgggcg
    15721 agggcgggga agacgaagcg aggttccagc gcgatcccaa cggcgactgg cggcgcagtg
    15781 ccgtcaagca ggtggcttcc ggccgcttcg gcgtgaccgc gaactacctc atcaacgccg
    15841 acgagctcca gatcaagatc gcccagggcg ccaagcccgg cgagggcggc cagcttcccg
    15901 gtcacaaagt ggacgaagtg atcgcgcgcg tgcggcactc gattccgggg gtgccgctca
    15961 tctcgccgcc tcctcaccac gacatctact cgatcgagga cctcgcacag ttgatctacg
    16021 acctcaagaa cgtcaatccg cgtgcgcgca tttcggtgaa actcgtagcc gaggttggtg
    16081 tcggtgttgt ggcggcgggc gtggccaagg cgcatgcgga cgtcgtgctg atcagcggcg
    16141 acagcggggg cactggcgcc tctcctctga cctccatcaa gcacgccggc atcccctggg
    16201 agcttgggct cgccgagaca cagcaggtgc tggtcatgaa cgatctgcga agccgcatcc
    16261 gagttcagac agacggcaag ctctcaaccg gccgcgatgt cgccattgcc gccctgctcg
    16321 gagccgagga gttcgggttc tcgacggctc cactcgtctc catgggttgc atcatgatgc
    16381 gcaagtgcca tctgaacacg tgcccggtcg gagtggccac gcaggatccg gcgttgaggc
    16441 gcaagttcag cgggacaccg gagaacgtga tcaactactt ctttttcgtc gccgaggagc
    16501 ttcgtgagct gatggcgcag atgggcttcc gaacggtcga cgagatgatc gggcgcgtcg
    16561 atatgctgaa gatgcgcgac gcgatcgacc actggaaggc acgcggcctt gatttcgcgg
    16621 cgatcttcca caaccccgag gtgccgcagc gtgtcgggcg gcgctgcctg gtcgctcagg
    16681 accacggcct tgagcgggct ctcgatcaca aactgatcga gctcgcccac ggtgcgatcg
    16741 agaacggcac tcccgtcgag cttgagttgc cgatcaagaa cgttcaccgg accgtcggcg
    16801 cgatgctcag cggagaggtc gcacgccggt atggatcgca agggctgccg gacgacacga
    16861 tccggctgcg cttctccggt tcggctgggc agagtttcgg cgcattcctg gcaaaaggga
    16921 taacgttcgt tctcgaaggc gacgccaacg attacaccgg caaggggttg tcgggcggca
    16981 ggctcgtcgt tttcccgccg cgaacgtcca cgttcgtgcc agaggagaac atcctggtcg
    17041 gtaacgttgc actctacggc gccaccagtg gagaagccta cttcaacggc cgggcgggcg
    17101 aacggttcgc cgtgcggaac tccggcgcca ccgcggtcgt cgagggggtt ggagatcatg
    17161 gctgcgagta catgacaaaa ggggtggtgg tcgttatcgg ccgcacgggg aagaatttcg
    17221 ccgccggcat gacgggcggc atcgcctacg tgctggacga tacgtctgag ttctcttcgg
    17281 cgctgtgcaa tacggaaagt gtcgatctgg aagccgttcc cgagggcgcc gaggccgata
    17341 gcctccagca gatcgtagcg cgccatttcc aggcaaccgg aagcccgcgg gtgggatggg
    17401 tgttggagaa ctgggaattt gtgcttccga aattcgtgaa ggtttttcct cacgagtaca
    17461 agcgggtact gggaatcccg cgagcgagcg ggcagccaag gacgatcctg ccgctcccgg
    17521 gttcgggaat ccagccgccg gcgcaagcgg tcttgggtgg ggactaggaa ggtgaaccat
    17581 gggcaaggtg accggattcc ttgaatacgc gcgtgacgtt ccacagcggc gcccggcaag
    17641 tgagcgtgtc aacgactggt tcgagatcta cctcgacttt ccggaggaga aagttcgcac
    17701 gcaaggcgcc cgttgcatgg actgtggcgt ccccttctgt cataccggct gcccgctgaa
    17761 caacatcatc ccggattgga acgatcacgt cttccaggac cgctggaggg acgcgattcg
    17821 cgtcctccat tcgacaaaca actttccgga gttcaccggc cgcgtctgtc cggcgccttg
    17881 cgagcccgcc tgcgtgctcg gcattaacga gcctccggtg acgatcaagg tcatcgagaa
    17941 aacgatcatc gaccacgcgt tccgccttgg gtggattcat ccggagcctc cggagcgccg
    18001 gaccgggaag aaaattgcgg tcatcggctc gggtccggcc ggcctggccg cggcgcagca
    18061 actcaaccgc gccggacact gggtgaccgt gttcgagaag gcggacagga tcggtggtct
    18121 cctccgttac ggtatcccgg acttcaagct cgagaaacgg catatcgatc gccggctcga
    18181 gcagttggcg gcggagggcg tccgcttcca gacccgcgcc cacgtcggcc acaacgtccc
    18241 ggtagaggac ttgcgggaag aattcgacgc cattctcttc gctatcggcg ctgaacaacc
    18301 gcgagacatg aatgttcccg gccgcgagct gaagggcatc catttcgcga tggactttct
    18361 gccgcaacag aacaagcggg tcgccggcga cgtggacatt gttgccgacc cgagccggtt
    18421 ctccgagccc gttctggcca ccggcaagcg cgtcgttatc atcggaggcg gcgacaccgg
    18481 cgccgattgc ctcgggactt cacaccggca aaaggcgctg tcggtgcatc agttcgagat
    18541 tctgccgcga ccgccggagg agcggtctac ttcgactcca tggccgctgt ggccgctcca
    18601 gttgcgtacg gagagctctc acgaggaggg tggcatccgg gcgtggagcg tggcaaccac
    18661 cggtttctcg ggcgacgagg atggcaacgt gcgcaagctt catggggtgc aggtcggtcc
    18721 gcccccgaag ttcgaagcgc tgccgggcac cgagttcacc ctcgatgccg acctgatcct
    18781 tcttgccatg ggatttcttg gtccggtccg aaatggagcg atcgaagcgc tcggcctcaa
    18841 acttgaccag cgcggcaacg tgcacaccga cgagaactac atggcctcgc tgcctggtgt
    18901 cttcgccgcc ggtgacgcgc gccgcggcca gtcactcgtg gtgtgggcga tcgcggaagg
    18961 ccgccaggcg gccaaggcca tcgaccgttt cctcacgggg gagacaaggc ttccgtagag
    19021 atgcctccgg cgcgatcggt tgagaccaac gccgccggat tcggcgccgc tttttcctcg
    19081 cctgtcctcg cggacccggt acagtatcat ggaaaaaatg acatcgccat cctcaacacg
    19141 ccaggaacaa tccgtaagag atacggtaat ggagttggag agagcgtacg ttctccagaa
    19201 ctacagccgg ttgccgatcg tcattcatcg tggcaagggc tgctacgtct atgattcgga
    19261 cggtaagcgc tacctggatc tcatcaccgg gatcggcgta aacgcgcttg gccacgcaca
    19321 tccccgcatt ctgagagtga ttcgcgaaca ggcttcacta ctgatccact cgtcgaacct
    19381 gtattaccac gagtatcagg gaccgctggc gaagagattg tgcgagatca gtggcctcca
    19441 gcgtgccttc ttctgcaact ccggcacgga ggctatggaa ggcgcgctga agatgatccg
    19501 atcgcacggc acgaagatca acccggagaa acacgaaatc atatcgctcg acaactcgtt
    19561 tcacggccgg acactcggtg cgatttccgt gaccggccaa gccaagtacc ggaaggactt
    19621 tgaaccgctg ctttccggcg tgaaattcgt ccctcccaat gattccgcgg ccctcgatgc
    19681 ggcgttcaat gaccgcacgg ccggcgtcgt tctggagtgg attcagggcg aaggcggtgt
    19741 ccgcccgctg cccgtcgagt atgtccgtca tgcgaaggaa ctggccgaac ggcataacgc
    19801 attgctggtc tttgacgaga tccagtgcgg cgtcggacgg cccgggactt acttcgccta
    19861 ccagcttgcc gagccggtga ttctgcccga cgtgacggtg gccgcaaagc cgcttgcttg
    19921 cggtcttcct ctcggagcgg tcatggcgaa cgacaaagcg gccgcgtcaa ttggtcccgg
    19981 catgcacggc acgacgttcg ggggaggccc actcgcctgc cgggttgctc tcgagttcct
    20041 cgacatcctg caggagcagt tgacccacat tcaccacgtc ggaggttatt tccgaatgga
    20101 gctctccgaa ctcgcgcgcc atcattcctt catcaaggat gtccgcggtt tcgggttgat
    20161 gattggtgtc gagttggaaa tgcccggtaa acagatcgtg actgacgcga tggaggaagg
    20221 gttgctcatc aactgcacgc aggagaacgt gctgcgattc cttccgcctt acatcatcac
    20281 ggaacctgag atcgaccggg ccgtcaaagt actcgcgaag atcttcaaga gggtcaagcc
    20341 ccctgcagca ggcgaaactg ccggggcgtg actcggtgcc tgcggttgtg ttgcggcgtg
    20401 atgctggccg agggccggta actacaacaa ctcctcccgc ttccgttttt ccaggagctt
    20461 gacgatgttg ccgacttgct gagaacggga ccggtcggcg atcagaaggg cgtccggagt
    20521 gtccacgacc accaggttct cgactccgag cagtgcgatc aaccggccct gcgactcgac
    20581 gtagttgccc cggctcgctt cgagcagcgt gtccccgcgc gtgacgttgc cctcagcgtc
    20641 gcgcgggagc agttcgtaga acgcgttcca acttccgata tcgtcccacc cgacgtcgtc
    20701 ggacgccagg ccggtcacgc ctgaggccct ctccattacg gcatagtcga ctgagatgtt
    20761 ctcgcacagc gggtaggtct cggcgagagc tccggcgaag cggcggctcg agaagggagg
    20821 tagcgcggca agcaaggcgg cggttttggg cagaaatttc cgcagagcgt cgaggtagac
    20881 ttccgcacgc cagaagaaca tgcctgcgtt ccagtagaac cggcccgcag cgaggaattc
    20941 acgagctgtc tcgatgtcgg gtttttcgcg gaactgtcga accggcaagg cttcaagact
    21001 gcctggacgg acacccctgg ggaactcgat atagccgtac ccggtctccg gccagcgcgg
    21061 ttgcatgccg agcaccgcga tgcgcccgcg gtccccttcg cggaacgctg gagtgagcag
    21121 gcgtctgtag cgagccggtc tgctcaccac atgatccgcc ggaaacacac ccatgacggc
    21181 gtcggaatcg atggaacgga ggatgtgcgc ggccaggccg atacagggcg ccgtgttgcg
    21241 ttgcgccggc tctgcgatga tctgtcgcct cggcacttcc ggcaactgcc ggacgatctc
    21301 ggcgcggagg tagtcgttcg tcaggaccca gatcctatcc ggagggatga gcggcttcaa
    21361 cctgtcgacg gtgtcctgga cgagggactt ctctccgaag aaccgtagca cctgtttggc
    21421 gttgccgcgg cggctccggg gccagaaacg cgtgcccctt cccccagcca tgatcaggcc
    21481 gtagtagttc gccttccgca tcggcctatc ctgtcagttg tcgaaagagt gcccgcggat
    21541 cagcttgaag gttctgcgcc atcgcgtctt ggtgaagaaa ttctggagca gatcgatcgc
    21601 gtgatccagg ctgatggttg agcttgccag ctgctcggtc ggtgcgtcgt aagaccagta
    21661 gatgatgagc ggctttccaa tgatgttctc ccgcgggacg aagccccagt accggctgtc
    21721 gaaagacgag tcacggttgt ctcccatcgc gaaataactg ctgggtggaa cgacaatttc
    21781 gctgttgacc acgtgtttct ctagcatctc ctgggcagct tcggagatcc tcgtgtttgg
    21841 ctcgctcggg aaattgtcgc gataggaatc gatgtactcc gtcttgaagt aggtgtacgg
    21901 ctcgttcagc ttttcgccgt ttcggaacac ctgcttgttg cggatatgga tacggtctcc
    21961 cgggatgccg accacgcgct tcacgtaggt ttgcgttacg tccatcggat accggaagac
    22021 gataatgtcg ccgcgcttga tctcctcgta cggaagaatg tatttgctga cgggccccgc
    22081 cggggcatag gcgagcttgt ccaccagtag atgatcgccg atcaacagcg tatcttccat
    22141 cgagccggtc ggaatcacga aagcttggac cagcgtcgtt gtcccgaaca gcagcaggat
    22201 aatcgttacg gtccattcgg cgatggtgcc gcgcggtcgt tcgcccgccg gtcgatgttt
    22261 cccggcggaa gcgtcgtcgg tcataacgag gttgtcttga ttcgccacga ttcctcgcaa
    22321 attctcattg taccggaagg atcgcaccct cgacgcggtc tcaagtcttt ggaccgcgaa
    22381 tcaactgcga cagctccacg agggatatgt agaggttcac caacccgatt ccacttacgg
    22441 cgccgcggaa atacggactt gtccacacca cgtaccactg agcgcttgtg ccggaaaaaa
    22501 gattctggct ccaggtctcg tgccacggca gaatgagcag gaaccctccg acctccgcgc
    22561 agaagaccgc cagcagcact cttgcgagaa tcctgtacca ccgatgcccc tgaggtttgt
    22621 tgttagccgg catctcggtt gctggcagtg cgcccaccgg tatctttggg cctcctagcg
    22681 gcattggccc gctgcctccg tgctgcatag tggaagtttc atctgatccc cgaaacggaa
    22741 cctccggacg ggcccgaacg taaagcggtg catcggggtt ggaccgaacg cctccagtgc
    22801 ctggacgtgc tccggtgttg ggtatccttt gcctgcccga aacccgtact gcgggtatac
    22861 cgcatcccac tcgtccatgc aggcatctcg atgcaccttg gccagaatcg acgcggcggc
    22921 gatggagcgg catcggcagt cgcctttcac cagcccgtct tgcggaatgg gaagatccac
    22981 cgacacggca tcgatcagca ggaaatccgg gcgcaccgcc agccgttcca ccgcccgctt
    23041 catcgcgagt cgtgaggcct gaaggatatt gatctggtcg atctcctccg cctcggcgga
    23101 cgccaccgcc catgccaggg caaaaaggcg aatctgttca gccagaacct cacgtttccc
    23161 ggcggctagc tgcttgctgt ctcgcagccc gcggatcggg tgatccggcg agagaataac
    23221 ggccgccgcg aaaaccggac cgaacagcga gccccggcct gcttcgtcga tgcccgcaac
    23281 catggcgtag cccctcgacc ggagcattcg ctcgtgactg ctgtcgcagc ggatgcgagc
    23341 tgttgcggcc cccgccaagt tattcgcgct cgcgcagtct tgccgccttg cctcgaaggc
    23401 cccgaaggta atacagcttc gcccggcgta cccggccggt gcgaactacg tcgatgtggt
    23461 cgatcaccgg agcgttcacc gggaagattc gttccacacc gtggccgaag ctgatctttc
    23521 gaacggtaat ggactcgccc attccggcat tcttgcgtgc gatgaccgtt ccctcgaaag
    23581 cttgaagccg ctccttgtcg ccctcccgaa tcttcacgtg tacgcgaatc gtgtcgcccg
    23641 gccggaaatc gggggcgtcc gtccgctttt tcttgacgat gaattttttc aacaacgtgt
    23701 cgctcatgac tgtgctacct tcattttctc caacagatcc ggacgcagcc gcgccgtttt
    23761 ctcccgagcc gctctgctgc gccatcgccg gatctcttcg tgattaccgc tcagtaacat
    23821 ctctggcgcg ctccagccgc gaaactccgc gggtcgcgtg tagtgcgggc aatccagtac
    23881 tcctaactgc ccgtcctcgc ccgtaaacga ttcaaacaca gccgagtcct cattgccgag
    23941 gacgcccggc agtagcctcg ccacggcgtc aatcaccacg gccgctgcaa gttcgccgcc
    24001 gctcaacaca tagtcgccga ttgaaatctc ttcgtcggcg agatgctcgg caacgcgctc
    24061 gtctacaccc tcgtagcggc cgcagatcag aagtaactcc tcgcaaccgc tcagccgctt
    24121 cgccgccggc tggtcaaaca gcctgccctg ggcggaaagc agaattacct tccggccggc
    24181 ctcccgctcg ggcaagatcg actccaccgc gagaaaaatg ggctctgcct tgagcaccat
    24241 tccctcgccg ccgccgaacg gtcggtcatc gaccgttttg tgccggtcga aggtccagtc
    24301 gcgcaggtta tgaacctgga cgctgaggat cccggactcg gccgcccgcg ccactacacc
    24361 gtgctccaac ggtccccgaa agaactccgg aaagatcgtg agcacatgaa acttcatctg
    24421 cgattcagtt ccttcagtcc atccggcagc ctgactgtga tctgcctctt ctccaagtca
    24481 atgttcacgc agatggaccg ggcaaacgga attaacactt cctcgccgtc cgcggttcgc
    24541 acgtccagca acccgctgcc accgaactcc tgccagtttg ccaccatccc gacagtactt
    24601 ccgctgtcgg cgtcgatgac ctcacaatcc accaggtcat cgtggtagta ttcgccgggt
    24661 ggaagttgat accgctccgt ccacgggacc cggatctcgg ctccgcgcaa ccgctcggcc
    24721 tccgagattg tgtcaatccc gcgaaacttg aagaccaccc gtccgcggtg ctcccatacc
    24781 gattcgacct cgtatggcga cccgcctatt gcggacccgt ctgcaccgaa caaatacact
    24841 ttttccagcc gagcaagccg ctcgatgcgg ttgctcatcg gaacggccgc cacttcgccg
    24901 cgattgccgc gagaattcac gatcgcggcg accgtaaccc accgtccccc agtgccggtg
    24961 ccagggcttg aaagcaggtc ctactccaga atttccagcg taaaccgccg gtttagcttc
    25021 attccggcag caccgaggat ggtcctgatt gagcgtgcgg ttcgcccctg cttcccaatg
    25081 acttttccca gatcacttgg ggcgaccttc agctccagaa ccgtcacctg ctcgccttgt
    25141 acttcgcgca cggcaacttg gtctgggatg tcgaccagcg ctttcgcgat ttcttcaacc
    25201 aattccttca tccccgccat ccctgcgcct cctactcagc ccaaatgata ccattcccgc
    25261 aggaagcgtt gcgctttcac cacgcgggtt cagttgacag attctgtagc aggtgtgggc
    25321 tccggctgcg ccttcagcag tcgagccacg gtgtccgacg gtttcgcgcc ggactttagc
    25381 cagtgagcaa tccgctcctg gtccaattga acggtcgccg gcttggtctg aggcttgtag
    25441 taaccaacga cctccaccga ccgcccgttt cgcgcccgat ctttctcaat tacaacaacc
    25501 cggtaagaag gtttcttttt ggcgccaaac cgcgccagtc gtatcatcag catgttttct
    25561 tgaactccgc aagtcttgcg gcggtcgggg tttcatcccc cgaaggcagg caacttgagc
    25621 ttcccgagcc gtttgcccat gaatccgccg gaaaaagtct tcatcatctt gcgcgcttgc
    25681 gcatattgct tgagtaactg attgacttcc tggaccgtcg tcccgctccc cctggcaatc
    25741 cgccgccgcc gggtaccgtt aataatcatg tggttcgccc gttccttcgg cgtcatcgag
    25801 tcgatgatgg cgacgacgcg gacgatctct ttctcgtcca ctttcacatt cttcaggtcc
    25861 ttcagcggcc ctacccccgg catcatcgaa agaatcgaat ccagcggtcc gagtttcttc
    25921 agttgtttga gctgatcgcg gaagtcttcg agcgtgaact cattgtcgat aagcttccgc
    25981 tgcatctcct cagcgttctt ctggtcaatg ctctcctcca ccttctcgat gaacgagagc
    26041 acatccccca ttccgaggat ccgcgacgca acccggtccg gatggaacgg ttcgagcgcg
    26101 tcgaattttt cgccaacgcc gacaaacttg agcggctgtc cggtaacgtg gcgtatcgac
    26161 agcgccccgc cgccacgggc gtcaccgtcc atcttggtca ggatcgctcc ggtgatgccg
    26221 agccgcttgt gaaattcatc ggcggacttc accgcatcct gccccgtcat ggcgtcggca
    26281 acgaacagga tctccgaggg ggacagtaac tgcttcagtt cctggagttc cgccatcaac
    26341 tgctcgtcga tgtgcagccg ccccgcggtg tccacaagga cgacgtccct gccggtctgg
    26401 atggcctcac ggcgggccga cttcgccagt tccgccggcg ccttctcttc cggagctcct
    26461 tcgtaaatcg gcaggcggag gtctctggcg attaccgcca attgatccct ggcggccggg
    26521 cggtagacgt ccaccgagac catcagtggc aaatgaccgt tccgcgaaag ccagcgggcc
    26581 agtttgccgg tcgtggtcgt ctttcctgag ccctggagac ccacgatgag aatcatcgtc
    26641 ggcggctcat tcgcgaaccg cagccgggac tggtgactgc cgagaagctt taccagctcc
    26701 tcttggacga tcttcaccac ctgctgggtc ggagaaagcg ccgtcagcac ttcctcgccc
    26761 atcgccttct gcctgacgcc ctccagaaac tgtttgacta ctttgaaatg gacgtcggcc
    26821 tccagcagcg cgacgcggat ctcccgcagc gccgcttcca tgttttcggg cgtcagcttg
    26881 ccttcgccgc gcaggttctt gaagacccgc tgtagcttat cggaaagatt ctcgaacatg
    26941 atcgaggtcc gaacgaacga cccggggtga gcgcaggccg actgcgactc ttctggggtg
    27001 tctattcatt ataaagggct tgccacccgc ttgtcagcaa ggacaggatt cctactgaaa
    27061 ctgccgcacg aagctttcga gccgtccgct cagatcctcc agcccgcttc gcagggattc
    27121 gacttccgtc tcgagccgag tgatccgctc ctcgatctcc gatctcgcgc caagccgggc
    27181 ttcggatcca cttgccgcct caacctccgg gacggctact tctccgccca gaaggtgtgc
    27241 gtaccggact tccctcatgc ccgcttgccg gggcagttgt acaaccaccg gctcgggctg
    27301 ccactcgatc agcctccgca ggcaggattc gacggattcc aggtcgtcga aagcgtgcag
    27361 cctctgcgct cgtgacttca gttctcccgg tgtctgtggc ccgcgcagca tcagttcacc
    27421 gagtatcgcc agttcccggt tgccaagatt cagcgtctcc gagattcggt gaccgtattt
    27481 cgggacgcgg tgctcacctc cggtcgtcac gagagccagt ctgcgctctc gcaggctgtc
    27541 ggcggcctgg gaaaccgtcg cttcgtcata gctcaccacg gggtgccggt tcgacttctg
    27601 gttacaagcg ttcaccaggg cgttcagcga gagggggtag tactccggcg tcgagaggtc
    27661 tttctcgatc agtgcgccca gaacgcgcac ctcgacttcg ttcaatgggc tgaagctcac
    27721 aaacggttcc ctttcttgcc ggcggccatt gtcgccgcca gcctcatggc ggccttcatg
    27781 ctgcccggat cggcgagatt cttgccggcg atgtcgaaag ctgttccatg gtccacggat
    27841 gtccggataa tcggcagccc caaagagacg ttcaccgtat gctcgaaatc gagcagcttc
    27901 atcggaatgt ggccctggtc gtgatacatc gccacgacaa ggtcatactc tccccgaacc
    27961 gcgcgcagaa ataccgtatc ggccggcgcc ggaccgatgc actggatccc gcgctgcatt
    28021 gcgacatcga tcgccggagc gatgaaccgt gcgtcctcgt cgccgaacag cccgtgttcg
    28081 ccggcgtgcg ggttcagccc gcacaccgcg atccgcgggt tcctgaaacc aagcagcctc
    28141 atggcctcct cgcccagctc gatcgtccgg agtatccggg ccggatccaa atcgcacgcc
    28201 ttccgcaacg gcacatgcgt tgagacatgt acgacgcgca ggcggtcgct ggccagcagc
    28261 attcgggact cgcgggcgcc gctcagttcc tcgatgaact ccgtgtggcc tgagaagcga
    28321 accccggcca tggtgaccgc ttccttgttg agcggggctg tgaccatggc gtccgcgatg
    28381 cctgttaggc acaattccgt ggcccttcgg acgtacccca gcgccgcccg gccgcaggcg
    28441 ccgctcagtc tcccggtctc gcactgtgag gcttcgagtt gccgcagatc ttcgcgacgc
    28501 gctgcctcgg gcgccggcat tccgagcccg gtgagcgcct ctgcccgccg gatcacccag
    28561 gcgtcgccta ccacgatcca gcgcgcgaga cacgtcacgc cttcatcggc cagggccttg
    28621 actacgacct ccgggccgac ccccgccgga tccccgatgg tcagcgctac tgtcggtttc
    28681 ttgttcattc cgtcgccgcc aaaaaatccg ccacccgtac cagagcgtct atttcgccga
    28741 atccgcctgc ttttgtggcc acaaccgtcc cgccggcaga cccgccgatg aggcgccccc
    28801 atggaatgcc gggcaggatc tcacgctcca accggattcc tcttgcctcc agcattccgc
    28861 acaccatcag cgcagtgtcg ccaccggtga gcacaattcc acgggcatct ttcagaatcg
    28921 cagaaaggtc cgcgacgcgc gacggagact gctccatcga gatgcgcatg atcggatacc
    28981 gcccacggag gtgcgcctct gcgtactccg cctgtctgac tgtaaccggg ttcgtggaac
    29041 cgacaatcag cgcgatcctt ctccggtccg cctccaggtt gggaacgcac gttacccctg
    29101 aatcggttgc tcgccctgtc agacaggcag ccagttcggc tgccagcccc gccgagcccg
    29161 caagcagagg acgcggccgc ataacccaag ccgcgcgtgc aatggcggcg aggtcggccc
    29221 gcgaggtggc gtcaagtaca actgtcgttc ctgcctcttc tcgcaggccg ccgagttggc
    29281 ctggcgtgcg ctggacgagc gtctctaggc cctgctcgcg gagtcgttcc gccacgtcga
    29341 ccgtagcgcc gaagtcgcgg acgtgcagaa gtccgttcat gacagtgcgt cccatctccg
    29401 gaaaagccgg cgtcacgaag gccagcggga attcgccctc aatccggacg gtttcgatct
    29461 ccggtccgag gttcccaagc agcgtcgagt cgatcttctt atagaggacc ctggccccgg
    29521 ctgcgcggag cacgcgacaa gcgtgtcgcg tcttcaccgc ggcggtctcg gcggtgtccc
    29581 tccggctatt ggtcgtgaca accgttacgt cggccgcctc gatttccgcg gcctccgcat
    29641 tgagccatac gatcgtccgc agcccgcgct gggcaaactg cactcccgcg tcgcacgcgc
    29701 ccgtcagatc gtccgcgacg acgccccacg gaacgttatc ctcgaagttc acttttcctc
    29761 gtggacgtga tcacgtcgct gggcttgtcc cgtgatcgcc cgggctccgc gccggcgcgg
    29821 cacgctcgtc gagcacctcg cggacgagtg tcttaagttg ctcgggatcg gcttggcccg
    29881 gagcccctgc caagtacaac gtgcgctgcg gaaacgggat ctcgattccg agttcgtcga
    29941 atcgcttctt gatgcgccgg ttcatttcgc ggccgacggt ccactgctgg atcggcaacg
    30001 tcttgatgcg tgccttgatg attacagcgg actcggcgaa tcgatcgacg ccgagcacct
    30061 cgagcggctc aaggatgaga acgttgtacc gatcctcctt catcatctgg gccgcgatgt
    30121 ccttcaagac cgccatgaca ttgtcagtgt cttctttgta agccacaccg atgtcgagaa
    30181 cgtagtagga atactcgcgt gtcatgttgg acaggctggt gatgacgccg ttcggaaaga
    30241 catgaaccgt gccatccgcg ccgcgaagaa cggtggtgcg cagattgatc tcttccacca
    30301 gaccgctcgt cccgttgatg acggcaacgt cgttcactcg gacctggttc tcgatgagca
    30361 tgaaaagtcc ggagatcgtg tcccgcacca ggttttgcgc gccgaatccg actgccaggc
    30421 cgaccacgcc ggcgccggca aggatcgggc cgatgtcgaa tcccgcctct ttaaggctca
    30481 tcaccagcgc gccggtccag ataagcaccg atagggttcg ccggaagatc ccccctaatg
    30541 tcgctgcctg cttttcaagc tcaccgtggc tcccggcacc gtgccggagc atcgaatcca
    30601 cgatccgttg cttcaacttc ggcacccact tgttgatcat ccgcacaagg atgaacgcca
    30661 ccaccaggat catgacgatc cggaccgccg cgacgagagc cagtgagagc ctgtcgagcg
    30721 aaaagagggg cagactgctc atgggaccat cctatcatcg gggtcgtcat cggacgccgt
    30781 gctagtaccg ttccaactgc ctgcaccgcg aagacgcttt ccgccgagag cgctatctga
    30841 agaccggcag gggaaagcgt taccttcggc agtggctcgc tgcgtggcgc gggcttctta
    30901 gccgggaaaa gttggaacgg cgctagacga tcagcatgct gtcgccgtaa gagtaaaacc
    30961 ggtatccggc ctccacggca tggcgataag cggacaggac cagatccttt ccggcaaacg
    31021 cgcacacaag cagcaaaagg ctgctccgtg ggaggtgaaa gttggtcagc atggcgccgg
    31081 t
//
LOCUS       DATIFQ010000452         2471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2068908, whole genome shotgun sequence.
ACCESSION   DATIFQ010000452 DATIFQ010000000
VERSION     DATIFQ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068908"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VLE22_22715"
     CDS             <1..469
                     /locus_tag="VLE22_22715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17281.1"
                     /translation="TLPLWSLPAPETFAPAATVLSQAFITALFQVCLFAIAIAATARY
                     FPGWKRYGPVAALALLYASGRAQSPLQFIYFSVTAAAGILVIEALVRLCAADLLTFGV
                     ALFWLHTIGPAVTLSEQPAAFLRWNGVACVALAVVLGIVVARKFPSVRVSASE"
     gene            496..1452
                     /locus_tag="VLE22_22720"
     CDS             496..1452
                     /locus_tag="VLE22_22720"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSB17282.1"
                     /translation="MRVLVTGAQGCIGAWVVKVLLDRGLDVVTYDLDPNPVRLSLIAP
                     PETLGRVRIETGRIEDTAGIKALVKGGGITHIVHLAAVLMPFCQAQPVAGAMVNVIGT
                     LNVFEAARDAGRDVRIVYASSSAVWGPREAYGDRMLSEDDPLKPATHYGVFKQSNEGN
                     ARVFHATNGITSVGLRPWSVYGVGRDSGLTADPTLAMKAVAFEQPFRIRLTGHMDLQY
                     VEDVAETFVRCLLDPLDGAFVFNLAGDIIAMSDLIATLERLRPGAGRLISAEGPEVPV
                     AYKMDDSQLRAKVPNIPKTPLETGIRRTLELFERLRAEGRLS"
     gene            1484..1738
                     /locus_tag="VLE22_22725"
     CDS             1484..1738
                     /locus_tag="VLE22_22725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HSB17283.1"
                     /translation="MRRTQLYLEEGVWEALHLRAKQEGVTISELVRKAVRERYVENAE
                     QRKQAMRAFAGIRRDRSDIGDTGVYVRKLRRGSRLHRLAR"
     gene            1738..2112
                     /locus_tag="VLE22_22730"
     CDS             1738..2112
                     /locus_tag="VLE22_22730"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB17284.1"
                     /translation="MAILIDSDVLIEVSRGRDRNLLSKWSELGDSDQALLCSPVTVAE
                     LWHGARPEEYAVLDALFAVLACVPIDAEIGRLAGDYLREFAKSQGVELGDALIGATAT
                     AHRVALWTRNRKHYPMRGLKFY"
     gene            complement(2119..>2471)
                     /locus_tag="VLE22_22735"
     CDS             complement(2119..>2471)
                     /locus_tag="VLE22_22735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17285.1"
                     /translation="AAIRLADQPAAADKTRRPPVSTPDSWKEKRGDTRIEGTLRQIDC
                     LGSSARFHVRTADRSLALYVANPGEVLLRNASSVTFEFRCGPQKPTPVSVEYNIRSDA
                     TLGTDGDVVAIEFR"
BASE COUNT          426 a          745 c          816 g          484 t
ORIGIN      
        1 gacgctgccg ctatggagcc tgcccgcacc cgagacgttc gcgccggcgg ctacagtgct
       61 aagccaggcg ttcattacgg cactgtttca ggtctgcctg ttcgccatcg cgatcgccgc
      121 gactgcccgg tattttcccg gctggaagcg gtacggaccg gttgcggcgc tggcgctgct
      181 atacgcctcc gggcgcgccc aaagcccgct gcaattcatc tacttcagcg tcaccgcggc
      241 ggcgggaatc cttgtcatcg aggcactcgt gcggctctgt gccgccgacc ttctcacctt
      301 tggtgtcgcc ttgttctggc tacacactat cggccccgct gtgacgttgt cggaacaacc
      361 cgcggcgttc ctgcgatgga atggggtggc gtgcgtggcg ctcgcggtgg tgctgggaat
      421 cgtcgtggca cgaaaattcc catcggttcg tgtctccgcg tcggagtgaa gcgacacgaa
      481 gatcctggag gaagcatgcg agttcttgtt accggcgcgc agggatgcat cggcgcctgg
      541 gtggtcaagg ttctgctgga ccgcgggctg gacgtggtca cttacgatct tgatccgaat
      601 cctgttcgtc tgtccctgat tgcgccgccg gagactctcg gccgggtccg aatcgaaacc
      661 ggccgcatcg aggacacggc ggggatcaag gcgctggtga agggcggcgg tatcacgcac
      721 atcgtccatc tggcggcggt gctgatgccg ttctgccagg cgcaaccggt ggccggggcg
      781 atggtgaacg tgatcggcac gctcaacgtc ttcgaggccg cgcgcgacgc cggccgggac
      841 gtgcggatcg tctatgcgag ctcttcggcg gtgtggggcc cgcgcgaggc gtatggcgat
      901 cgcatgctct ccgaggatga cccgctgaag ccggccacac attacggcgt cttcaagcag
      961 tccaacgagg gcaatgcccg ggtgttccac gcgacgaacg ggatcacaag cgtcgggctg
     1021 aggccttggt cggtgtacgg ggtagggcgg gactccggcc tcaccgccga tccgactctg
     1081 gccatgaagg cggtggcgtt cgagcagccg tttcgcatcc ggctcaccgg ccacatggac
     1141 cttcaatatg tcgaggatgt tgccgaaacg tttgtgcgct gtctgctgga tcccttggac
     1201 ggggcgtttg tcttcaatct tgccggagac attatcgcga tgagcgatct catcgccacg
     1261 ctcgaacgcc ttcgtccggg tgcgggccgt ctcatctcgg ccgaagggcc ggaagtaccc
     1321 gtggcctaca agatggacga ttcacagctc cgggcgaagg tgcccaacat cccaaagacg
     1381 ccactggaaa ccggcatccg gcggacgctc gaactgtttg agcgactgcg agccgagggc
     1441 aggctatcgt aggagtggac ttgggcgatg tataatatac attgtgcgac gcactcagct
     1501 ctatcttgag gaaggggtct gggaagccct ccacctgcgc gcgaagcagg agggggtcac
     1561 catctcagaa ctggtccgaa aggctgtgcg tgagcggtac gttgaaaacg cggagcaacg
     1621 gaaacaggcg atgcgtgcat tcgccggtat caggcgtgat cgttccgaca tcggcgacac
     1681 cggcgtttac gttcggaagt tgcgccgagg cagccggctt cacaggctgg cgcgctgatg
     1741 gcaatcctga tcgactccga cgtcctcatc gaagtctctc gcggcagaga ccggaatcta
     1801 ctgtcgaagt ggagcgaact cggtgattca gatcaggcgc ttctctgctc tccggtgacg
     1861 gttgccgaac tctggcatgg tgcccgcccg gaagagtacg cggttctcga tgcattgttc
     1921 gcggtgctgg cctgcgtgcc gattgatgca gagatcggac ggctcgcggg agactatctt
     1981 cgcgagttcg cgaagagtca aggcgtagag ttgggcgacg cgctgattgg cgccactgcg
     2041 acggcccacc gcgttgcgtt gtggaccaga aaccgcaaac actaccccat gcgcggcctc
     2101 aagttctatt gacctgcttc accggaactc gatcgcaacc acatctccat cggtccccag
     2161 cgtggcatcg gagcgaatgt tgtattccac ggagacggga gtgggctttt gcgggccaca
     2221 ccggaattcg aacgtcaccg aggaggcgtt tcgcagaagc acctcgcccg gattcgcgac
     2281 gtaaagcgcc agactacggt ccgccgtccg cacatggaaa cgggccgagg aaccgaggca
     2341 gtcgatctgc cggagcgtgc cctcgatgcg agtgtcgccc cgcttttcct tccaactgtc
     2401 cggcgtgctg acgggagggc gtctcgtctt gtccgcggcc gccggttggt ccgccagtcg
     2461 gatggctgca c
//
LOCUS       DATIFQ010000453        17404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_344664, whole genome shotgun sequence.
ACCESSION   DATIFQ010000453 DATIFQ010000000
VERSION     DATIFQ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17404
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344664"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VLE22_22740"
     CDS             <1..771
                     /locus_tag="VLE22_22740"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSB17286.1"
                     /translation="RQPGAPLRPGFNLFSQEQDIQLGREAAAEVRKQVDVVENSELQS
                     YIAELGSRLSRNPDAGAYPYSFTLVNDPTLNAFALPGGPVFVHTGILAAAENEAQLAG
                     VMAHEISHVALRHGTNQASKANIAQLPAVLAGAAVGQGSTLAQLAQLGIGLGFNSLLL
                     KYSRDAEREADSLGAHLMAQAQYNPIEMARFFEKLEAEGGSRAPDFLSSHPSPGNRVK
                     NVEAEIRTLPASSYTEGTGRLARMKALVSQLPPPRKRS"
     gene            863..1927
                     /locus_tag="VLE22_22745"
     CDS             863..1927
                     /locus_tag="VLE22_22745"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB17287.1"
                     /translation="MGVTRRGFLGESVLLAGGFAAGVGSASGPRILNQNENMEYRRCG
                     KTGLMVSAVCLGGHWKRIETVVPDALKTKNWLNVDVAHGEFAKNRHDVVSRCIDVGIN
                     YIDACTGPEVMAYAAALRGRRDKMYLGYSWYDREVRYPDWRCTEKLLQGFDDGLLAAG
                     LDYVDLWRITCHEPGGQHTFNESEALVTALDKAKRQGKARFTGISSHDRRWLKMMIEQ
                     YPEQIDVILTPYTADSKALSKDSLFDTVRKYGVGVFGIKPFASNALFQGDSRPGGPHA
                     EEDNRRARLALRYVLGNAAITAPIPGMVNIEQVDNAAKAVMERRQLDEKETADLKQAA
                     GEMWANLPDHYEWLKNWEYV"
     gene            complement(1928..2608)
                     /locus_tag="VLE22_22750"
     CDS             complement(1928..2608)
                     /locus_tag="VLE22_22750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17288.1"
                     /translation="MPSSVEQLAVSKTLSRRLVDAFEVWNRKLHFYIGLYLLFFLWLF
                     SFTGLLLNHSGWKFAEFWPNRRQSDFVRPIQPPPPGGDLAQAKDILRQLGIEGEIDWT
                     NTRTDLSCFDFRAGRPGRMFEIQTDLQRNVATVHRTDINMWGVLHVLHTFTGVRMADS
                     RNDRDWALTTVWALSMDALAVGMILMVVSSLYMWWRLKPKRRFGALALGLGLASCGLF
                     VIGFRWIL"
     gene            complement(2592..3221)
                     /locus_tag="VLE22_22755"
     CDS             complement(2592..3221)
                     /locus_tag="VLE22_22755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17289.1"
                     /translation="MNKRFYTVTRDLHLYIGLFLAPFVLVFAVSVFFLVHSWLPGVAP
                     EPGPPRTVTGVLIPNGVENLDGRKRVDAVRAVLDPLGVAGEVTVIRHIRNERRLVIPV
                     IVPGRETTVDINLETRTALVSTRTTGIWDALVTLHKAPGPHLVAIRMNWFPMRVWKWL
                     ADATVWLVLFLSVSGIYLWAVLRAERRIGLVLIAAGATSFFGVIYALVR"
     gene            3596..4426
                     /locus_tag="VLE22_22760"
     CDS             3596..4426
                     /locus_tag="VLE22_22760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17290.1"
                     /translation="MDSLQNERTKGSSVRGGGEADVIVRVDRVKTEEDAAATRMRLRK
                     LAEQIAQGVTSCWSEAANDANRLAPIDQEKLAQLSTHLRNVSADVRDLRSEHELKVER
                     TENLAQNQEDLSARLALSEERFLEVATITRRIEKSMRRATEAQEELREQIVNQYAAIC
                     RLEQAVRTHTESIENRLTDAVAQIAHRVESVVRAQEVHADTIAKLGAAWSHINDLQQS
                     FEQRVEGQARAIQSIHSATRAQAALWAELKLTAVHVREAIRIPSGELLTPSETDLERL
                     "
     gene            4540..4956
                     /locus_tag="VLE22_22765"
     CDS             4540..4956
                     /locus_tag="VLE22_22765"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB17291.1"
                     /translation="MFTTRKKEELQRSDTTEGPTPVSRATPDRRTSGRFPIVRDVRYK
                     IVRSKGTSEVGEGKTVDISSTGVLFTAETPLPLGKQLELSISWPAQLRGKCSLKLVAR
                     GRIVRCQGTAVAIEIDAHEFRTAGREGTLPREAQPS"
     gene            complement(4975..6603)
                     /locus_tag="VLE22_22770"
     CDS             complement(4975..6603)
                     /locus_tag="VLE22_22770"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB17292.1"
                     /translation="MSTATFAPGKAAEFQVIQPRTTYTGSAIPSVPKGLPKLTISLCP
                     ECTGKIEAVLFEEDGKVIMEKTCPEHGLFRDVIYSDVKLYLKMEEWTFGDGRGIENPA
                     VPEATNCPDQCGLCSMHTSHTVLANVDLTNRCNLTCPVCFANANAAGYLYEPTFDEVR
                     KMLQTLRDQRPVSGRVVQFSGGEPTIYPRFVDVLKMAKEMGFTQLQAATNGLKFTDPE
                     FAQQCGEAGLHTLYLQFDGVCDDIYKRTRGESLFEMKLKCIDNVRKAGMKICFVPTIV
                     KGINDHQIGDIVRLAIDNIDVVSAISFQPVSFCGRISKAELEEKRFTQSDFAHAVREQ
                     TGLADPYNDWFPLSCVSPFSKLIAALRGETVPTLTPHPHCSMGTYMFVDKNKHATPIT
                     RFVDIGAMLQDIDMLARRAEQRRIKFWSKISAWNSLRKHYHEDRAPEGLTFQRFLQTL
                     QGLVDKKYGRGEMEKQEFSYKTLMVASMHFMDVYNYDVERVRRCVIHYAAPNGQLYPF
                     CAYNAGPTFREKIEKAYSMSFEQQPRMQELINIG"
     gene            complement(6849..8960)
                     /locus_tag="VLE22_22775"
     CDS             complement(6849..8960)
                     /locus_tag="VLE22_22775"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB17293.1"
                     /translation="MHKPIKYFEKAVALTASGAWAVFSRLNQIGRNPSFTPKWSDKPL
                     LKSWEKQKPPLGWPRETDSLCPKCVPEIRQEILDGKKPLEVLIHDKPGEIKAQIIERD
                     GQIWMVKDCPKHGHFEDIMSIDAEFMKHIESVFPGRDIRAHNDERLHHHGSSTVTHGR
                     GAVLTIDLTNRCNMMCDPCFMDANQVGFVHELTWEEIKTLLDNAISIKPKRQMSVQFS
                     GGEPTLSPYFLDAVRYAREVGYTSVQAATNGIEFAKSVEFCKKAAEAGLRYAYLQFDG
                     IGNAANSHRKVGNLFDVKLQAIENLYKAGVDIVPVITIVNGINDEQVGRVVQFALDNP
                     KKIPFLSFQPVSFTGRDEAVTNERRAAQRYTLSHLAYDVKKQTGIGEPRRDWFPLSFM
                     STFSDWSDLVHGPEANWGQLSCGCHPNCGLGMAVLVDKETKEAVPFTAFLNADQLAKD
                     VMQINDAARGRWLSIFGMALAVARNYDPFQTPTHFKLTDMLKKMDKTFGATGKSYGKV
                     GADRTLEDIERRRRDRWNVLMIAGMWFQDLFNYDFRRTEQCIIPYATQEGEISFCAYN
                     TGVGWRNIVERIHMTATLTKWYDEHGKHEIFAGGKRVELARTEHSLTLREEIITREEQ
                     HDLDRLGIAKNAREEKARARNEKMRQEAEHNTRMAKLYREVVLKEKPAEPLVQINSLN
                     GNGNGHTAKQAEQEREEVFTD"
     gene            9203..10273
                     /gene="queG"
                     /locus_tag="VLE22_22780"
     CDS             9203..10273
                     /gene="queG"
                     /locus_tag="VLE22_22780"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HSB17294.1"
                     /translation="MKTEAMPVSAKRVKEIAAECGFEIVGVASAAPLSEHAWYREWAA
                     LGMAGRMSYLTDRRGDARQDPRRLLASARSVLCVGKLYNAPKPYSVDFEAVELGWISR
                     YAWGEDYHRVVADGLHRVVARLKSSGAGPFDWKVCVDTAPLLERALARRAGLGWIAKN
                     TCLIREGMGSWFFLGELLLSLDLESDSPPDERCGSCTRCIDACPTRAIVPAGRAEPAW
                     AIDSRLCISYLTIELRGPWPHELRGAAGLNVFGCDICQDVCPWNTNAPVTPDSAFEPA
                     NFAPPLERMAALTEEEFVAMFRGTPVTRAKYTGFLRNVAVAMGNSGRPEFRAPLERLA
                     QHPDPAVADHAGWALAHIGRIK"
     gene            10282..11463
                     /locus_tag="VLE22_22785"
     CDS             10282..11463
                     /locus_tag="VLE22_22785"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17295.1"
                     /translation="MLLVFLLLLCSVVASPASEATLSKGFDHFYNLEYDEAIAEFRKL
                     IDRNPESAGARNHYAQAILYREMFRAGALESELVSGTNPFLRRAKMNPSAEDQRHFDE
                     SVAKAIELSDARIKKDPNDSQALYALGVTYGLRANYNFLVRKAWFDALRDATRARKLH
                     NRVTELDPKIVDARLVEGVHDYVVGSLPWHVKMLGFLVGFRGDKEGGVRLLEQVAQHG
                     DINRVDAQILLCAIYRRERNPQKAIPMLLSLIERFPRNYLLRFEIAQMYSDLGDKPNA
                     LAALRRIEELKSGGASGFSHLMPEKILYARGTIQFWYNDLDRALEDMRRVTSRAGDLD
                     LNTGTFAWLRLGQIYDLKGQRAQAVAAYRQAINLAPQSDAARLSTRYLSEPYRRDKRK
                     S"
     gene            complement(11498..11845)
                     /locus_tag="VLE22_22790"
     CDS             complement(11498..11845)
                     /locus_tag="VLE22_22790"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSB17296.1"
                     /translation="MRLSFISLALIAFLVISSVLVAQGAFPRMTAVEPDTGKAGDVLN
                     VAGENLGKETVAQVFLTDGKTDTKVEITEQAATSIKIKVPASLKTGRFSLMVLTTEKQ
                     PKLIEQPVKVNIE"
     gene            11992..12471
                     /locus_tag="VLE22_22795"
     CDS             11992..12471
                     /locus_tag="VLE22_22795"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSB17297.1"
                     /translation="MTKLSAGFLMFRGSQGAVEVLLVHPGGPFWEKKDLGSWSLPKGE
                     YPEGEEAFDAAMREFEEETGIRPEGPFIPLGQIRQPGGKLVTVWAFEGDCDPATIKSN
                     LFSMEWPKGSGRMQEFPEIDRGGWFTLTVARRKILKGQVGFLDRLAEHLDVRKLFVP"
     gene            complement(12457..14919)
                     /locus_tag="VLE22_22800"
     CDS             complement(12457..14919)
                     /locus_tag="VLE22_22800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17298.1"
                     /translation="MWVLLQDLRYGLRMLAKNPGFAAVAVIALALGIGANTAIFSVVN
                     GVLLQPLPYRDPDRLVRLSERSPGFETMSVAYPNFVDWNDQNRSFAGLAAFRWEDYDI
                     TGGGEPEHLSGKMVSADFFRVLGINPVAGRDFDPRADRLGASPVVLIGGGLWSRRFGS
                     SPAVLASQLTLNGRSYTIAGVVPAGFQFEGKADIYTLLEQWDDMLARSREMHPGMYVV
                     GRLKAGVTRTQAQSEMTAIAARLAEAYPKSNSKHGVNIEPLTGVIVGNVRPTLLVLLG
                     AVGFVLLIACANVANLLLARFTRRHKEIAIRSALGAGRVRVVRQLLTESVVLALAGGI
                     AGLAIARWGTQAVLAAVPGGLPRVENIGVDGWVLAFTLGVSLLTGIVFGLAPALHVSV
                     ADLHETLKEGSRGSSARHQRLRNLLVVSEVAAALVLLAGAGLMLRTIWNLGSIKPGFK
                     PDHVLTFSVGLSPANMSSTDRILQTFEQTADRIQSVPGVKSAGVSSLIPLGGSDSEIP
                     FYVNGRPRPTSQGDMSWALLYATDPGYLKAMDIPLLRGRYIERQDMHRGSHVVVIDEV
                     MARTFFPHEDPLGKSIVVADLSGDLGPEASVPMEIVGIVGHVSHWGLDSDASARVRSE
                     LYLPLSQIPDQFMKAMAGSSVFVVRTGVDPLATFPAVRSAVLEAGNDQTVYGVRTMEQ
                     IVSGSIADRRLSTLILGIFAGLALLLAAVGIYGVISYTIAQRTREIGIRMALGAGQAD
                     VLKLVLAQGMFPVLAGLAIGVAASFGLTRLMAGMLYGVNAGDPVTSIGVALVLAAVAL
                     IATLVPARRATRVTPVVALRYE"
     gene            complement(15028..15438)
                     /locus_tag="VLE22_22805"
     CDS             complement(15028..15438)
                     /locus_tag="VLE22_22805"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HSB17299.1"
                     /translation="MAKTDWKSVDEYIASQPETVQSVLECVRSTIRKAMPGAEEVISY
                     QIPAYKLHGRVAIYFAGWKQHYSLYPATGHLVTAFKDELASYEVTKGTIRFPLSEPVP
                     VRLIGRIAKFRAKEAAEREKLMAATAKKRQARAV"
     gene            15701..16102
                     /locus_tag="VLE22_22810"
     CDS             15701..16102
                     /locus_tag="VLE22_22810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17300.1"
                     /translation="MKAKMAVLILLLAATSALARTRVSIGVYVGDGYGYGYYPPPPVY
                     VYEPPCPGPGYVWVPGYWYGVGPRYSWRNGYWAPPRYRGWYRGGPRYRYYDAWDSPYR
                     GRMKYRKHDDRGWGRGRYRDYYRYDRRGYRR"
     gene            complement(16126..>17404)
                     /locus_tag="VLE22_22815"
     CDS             complement(16126..>17404)
                     /locus_tag="VLE22_22815"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HSB17301.1"
                     /translation="ALARHCAELYDFLKSANPKLAVGFNLKGIYGVNRIWRNAVYHPF
                     FEGKIDFSPFDVGGMDARLDSRTGAMVSEIRSYKMARTLNFSYQEGGNALEFALCMAF
                     NPQKYVPGYGYEGGPSGWGAARAFSPEAEFFREYNDRYYTETENVADVAVVRTWPSMA
                     YSVAATLVPTILIEQVLIQHKVPFDILFDGQIDRIDRYRAVILPGQESLSQEWVDRLS
                     AYVRGGGTLVFTGNTADFNERRERRAANPLLALMGAPARPAAITVKTVGQGKVVCIPE
                     VGRQARGRATAAGDETEVEVATGGRADFPASQWLLPPNHEEIHHAIADNARGGLSIAS
                     AAPLNTVMELLNRKKSSETIVHFVNFEQGKRLDSFEVRLRKQFPGKVSSVKVFSPDSD
                     EPISAQFKEEAGRVVFQVPSMGLYSMAVVSHQP"
BASE COUNT         3396 a         5178 c         5453 g         3377 t
ORIGIN      
        1 cgacaacccg gggccccgct tcgccccggg ttcaacctct tctcacagga gcaggatatt
       61 cagcttgggc gtgaggcagc ggccgaggtg cgcaaacagg tggacgtcgt ggagaactcc
      121 gaactccaga gttacatcgc cgaactgggt agccgcctct ccagaaatcc ggacgcaggc
      181 gcttatcctt acagcttcac tctggtgaac gaccctacgc tcaacgcctt tgcgctgccg
      241 ggcgggccag tgttcgtgca cactgggatt cttgcggctg cggagaacga agcgcaactt
      301 gccggcgtta tggctcacga aatttcgcac gtcgcccttc gccacggcac caaccaggcg
      361 tcgaaagcaa acatagctca actgcctgcg gttctggcgg gcgccgcagt cggacaaggg
      421 tcgaccttgg cgcaacttgc acagcttggc atcggcctgg ggttcaattc gcttctgctg
      481 aaatactcgc gcgatgctga gcgggaggcg gattctctgg gagcgcacct gatggcacag
      541 gctcaataca acccgattga gatggctcgg ttcttcgaga agctcgaggc tgagggcggc
      601 tctcgggcgc cggattttct ctcctcgcac ccgagccccg ggaatcgcgt caagaatgtg
      661 gaggccgaga ttcgaacctt gcctgcttct tcctatacgg aaggcacggg acggctcgct
      721 cgaatgaagg ctctggtatc tcaacttccg ccgccgcgca agaggagcta agcccgaggg
      781 ttgtgttatc ggccattgac cgtgccttca ccgtgacgta cactgcattt gcccggtgaa
      841 acggattcac ggaggtgaat gtatgggcgt cacgcggcgc gggtttctag gcgagtcggt
      901 tctcttggcc ggagggttcg cggccggcgt gggttcggcg agcggtcccc ggatcctcaa
      961 ccaaaacgaa aacatggagt accggcgctg cgggaaaaca ggactgatgg tgtccgcggt
     1021 ctgcctcggt ggccattgga agcgcatcga gaccgttgtt ccggatgcgc tgaagacgaa
     1081 aaactggctg aacgtcgacg tcgcccatgg cgaattcgcg aagaaccgcc acgacgtggt
     1141 gagccgctgt atcgacgtcg ggatcaatta catcgacgcc tgcaccggac cggaagtgat
     1201 ggcctatgcc gcagctctgc gtggccgccg cgacaagatg tacctcggtt actcctggta
     1261 tgaccgggaa gtccgttacc cggactggcg ctgcaccgag aagttgctgc aaggtttcga
     1321 tgatggcctt ctcgccgccg gacttgacta cgtggatctc tggcgaatca cctgccacga
     1381 gccaggcgga cagcacacgt tcaacgagtc ggaagcgctc gtcactgcgc tcgacaaggc
     1441 gaaacggcag ggcaaggcgc gcttcaccgg catctcctcc cacgaccgcc gctggctgaa
     1501 gatgatgatc gaacagtacc cggagcagat cgacgtgatc ctcactccct atactgccga
     1561 cagcaaggct ctttcgaagg acagcctgtt cgacacggtg cggaagtacg gggtgggcgt
     1621 cttcggaata aagccgttcg ccagcaatgc gctgttccag ggggacagca ggccgggcgg
     1681 tcctcacgcc gaagaggaca atcgacgcgc acggctcgcc ctccgttatg tgctgggaaa
     1741 tgccgcgatc accgcgccga ttcctgggat ggtcaatatc gagcaggtgg acaatgccgc
     1801 gaaagcggtc atggagcgcc gccaactcga tgagaaagag acggccgacc tcaagcaggc
     1861 cgcgggagag atgtgggcca atcttccgga ccactacgag tggcttaaga actgggaata
     1921 cgtgtagcta cagaatccag cggaagccga taacgaacag tccgcagctc gcgaggccca
     1981 gtcccagcgc aagcgcgccg aaccggcgct tcggtttcag gcgccaccac atatagaggc
     2041 tgctcacgac catcaggatc atgcctactg ccagggcatc catggaaagc gcccacactg
     2101 tggtcaaagc ccagtcgcgg tcgttcctcg agtcggccat ccggacgccc gtgaacgtgt
     2161 ggagaacgtg caggacgccc cacatgttga tatcagtgcg atggacggtg gcgacatttc
     2221 gctgaaggtc cgtctggatt tcgaacatgc gcccgggccg gccggctcgg aagtcgaaac
     2281 agctcagatc ggtgcgcgta ttcgtccagt cgatttcacc ctcgattccg agctgccgca
     2341 ggatgtcctt ggcttgggca aggtcgcctc ccgggggtgg aggctgaatc ggccgcacaa
     2401 agtcggactg ccgccggttc ggccagaact ccgcgaactt ccagccggag tgattcagta
     2461 acaatccagt gaaggaaaac agccacagaa agaacagaag gtagaggccg atatagaagt
     2521 ggagcttgcg gttccacact tcgaaggcgt ccaccaaacg gcgggagagc gtcttgctga
     2581 cggccagttg ttcaacggac gagggcatag ataactccaa agaacgacgt ggcgccggcg
     2641 gcgatgagaa caagcccgat gcgacgctcg gcgcgcaaga ccgcccacaa atagatccca
     2701 ctgacggaga ggaacagaac tagccagacc gtcgcgtcgg caagccattt ccacacgcgc
     2761 atgggaaacc agttcatgcg gatcgcgaca aggtgggggc cgggtgcctt gtgaagcgtg
     2821 acgagcgcat cccaaatgcc cgtcgtgcgc gtggagacga gcgcggtgcg ggtttcaagg
     2881 ttgatatcga ccgtggtttc ccggccgggg acaatcaccg ggatcacaag acgccgctcg
     2941 ttgcggatgt gccggatgac ggtaacctca ccggcgacgc cgagcgggtc aagcactgcg
     3001 cgcacagcgt ccaccctctt ccgaccatcg aggttctcga cgccgttcgg aataagcact
     3061 ccagtgaccg tgcgcggagg accgggttcg ggcgccaccc ccggcaacca ggaatgcacc
     3121 aggaagaata cactcaccgc gaaaaccagg acaaaagggg caagaaacaa cccaatgtac
     3181 aggtgcagat cgcgggtaac ggtatagaag cgtttgttca tagcagcggc agtacaccct
     3241 cgggaactgc cggtaccgca caaccggcgt gcggatcctt cgtagctgag tcagtgacga
     3301 accaagtggt cattaatgtg accatcccct attatcgcga ctagcgcaag cgctttgttt
     3361 gcctgagcaa acggcgattc cgggtggcaa agtccagtct cgcgcacgat gtagggggtg
     3421 agcctggtac tgccgggagc gtaccttcgt cccagaaggt taaccggccg aaagaactcc
     3481 accctgagac tagagtactg gcaatacctc cgtgttgagt tctatcacaa aactaaccag
     3541 tgttccattg atcggagggg gcagtggcct tacgctaccg gtgagaggtc ggcgaatgga
     3601 ttcgctacag aatgaaagga cgaaggggtc gtctgtcaga gggggcggcg aagccgacgt
     3661 cattgtccgg gtcgaccggg ttaagacgga agaggacgcc gccgccacac gaatgcggct
     3721 tcggaaactt gcggaacaga ttgcgcaagg agtgacaagc tgctggtctg aggcggcaaa
     3781 cgacgcgaac cggctggccc cgattgacca agagaaactt gcgcagttgt cgacccatct
     3841 gcgcaatgtg tccgccgatg ttcgggatct ccggtccgaa catgaactta aggtcgagcg
     3901 gacggaaaac ctggcccaga accaggaaga tctgtcggcc cggctggcgc tctcggagga
     3961 gagatttctg gaggtcgcga cgatcacccg gcgcatcgaa aaaagcatgc gtcgggcgac
     4021 ggaagcacag gaagaacttc gcgagcaaat cgtgaaccag tacgcggcaa tctgcagact
     4081 ggagcaggcc gtcaggactc acaccgagtc gatcgagaat cgtcttacgg acgctgtcgc
     4141 ccagatcgct caccgcgtcg aatcagtggt acgggctcag gaggttcacg cggatacaat
     4201 cgccaagctt ggtgcggcat ggtcacacat taacgacctt cagcagtcgt tcgagcaacg
     4261 ggtggaaggg caggctcggg cgatccagtc aattcactct gctacgcgag cccaggcggc
     4321 cctttgggca gaactcaagt tgacggctgt ccacgtcagg gaggcgatca ggattccttc
     4381 tggcgaactg ctcaccccct ctgaaacaga cctggaaagg ctgtaagatg tggaccgggt
     4441 cgacgtttct aagtaagagg gatgcgttgt aatgaacgag gcgaagtgga gccacggagg
     4501 accgggctag atttcccata agaaccagcc cgggtgcgta tgtttacaac gaggaaaaaa
     4561 gaggagctgc agcggtcaga cacgacggaa ggtccgacgc ccgtgtcgag ggcaacacca
     4621 gatcgcagga caagcggccg gttcccgatc gtgcgtgacg ttcgctacaa aatagtaagg
     4681 tcaaagggaa cttcggaggt cggagaaggc aagacagtgg acatcagcag caccggggtg
     4741 ttgttcacgg ctgagacgcc cctgccgctt ggcaagcaat tggaactgtc gatcagttgg
     4801 cccgcacagc tccgcggcaa gtgctcgctg aaacttgtcg cccgcggccg gattgtccgc
     4861 tgccaaggca cagccgttgc catcgaaatc gatgcccacg agttccgcac agccggacga
     4921 gaaggaacgt tgccgcgcga agcacagcct tcgtgattat tggcaggcgt cggcttagcc
     4981 gatgttgatc agttcctgca tccggggctg ctgctcgaac gacatcgaat acgccttctc
     5041 gatcttttcg cggaatgtcg gaccggcgtt gtaagcgcag aacggataga gttggccgtt
     5101 cggggccgcg taatggatga cgcagcggcg cacgcgctcg acgtcgtagt tgtagacgtc
     5161 catgaagtgc atgctggcca ccatcagcgt tttataggag aattcctgtt tctccatctc
     5221 accgcggccg tacttcttgt ccaccagacc ttggagcgtc tgtaggaacc gctggaacgt
     5281 cagcccctcg ggcgcccggt cttcgtggta gtgcttccga agcgagttcc acgccgaaat
     5341 ctttgaccag aacttgatcc gtcgctgttc cgcccggcgt gcgagcatgt cgatgtcctg
     5401 gagcatcgcg ccgatgtcga cgaaccgggt gatcggcgtc gcgtgcttgt tcttgtcaac
     5461 gaacatgtag gtgcccatcg agcagtgcgg atggggcgtg agtgtcggaa ccgtctcgcc
     5521 gcggagcgcc gcgatcagct tcgagaaggg gcttacgcac gacagtggga accagtcgtt
     5581 ataaggatcg gccaggccgg tctgctcgcg aaccgcgtgg gcgaaatcag actgcgtgaa
     5641 gcgcttctcc tcaagctctg ctttcgagat tcgcccgcag aaactcaccg gctggaagct
     5701 aatggcgctc accacatcga tgttgtcgat cgcgagccgg acgatgtcgc cgatctggtg
     5761 gtcattgatg cccttcacga tcgtcggcac gaaacagatc ttcatcccag ccttgcggac
     5821 gttgtcgatg catttcagct tcatttcgaa caacgactcg ccgcgggtcc gcttatagat
     5881 gtcatcgcaa acaccgtcga actggaggta cagcgtgtgc agacccgcct ccccacactg
     5941 ctgggcgaat tcgggatccg taaacttgag gccgtttgtg gcggcctgga gctgagtgaa
     6001 gcccatctcc ttcgccatct tcagcacgtc gacgaaccgc gggtagatgg tcggctcgcc
     6061 tccggaaaac tgaacgacgc ggcccgaaac gggacgctgg tcacgcagcg tctgaagcat
     6121 cttgcggact tcgtcgaagg tcggttcgta gaggtaaccg gcggcgttgg cgttggcgaa
     6181 gcagaccggg caggtgaggt tgcaccggtt ggtgaggtcg acgttggcga gaaccgtgtg
     6241 gctggtgtgc atggagcaca gcccgcactg gtccgggcag ttcgtggcct ccggcaccgc
     6301 ggggttctcg atgccccttc cgtcgccgaa ggtccactcc tccatcttga ggtaaagctt
     6361 tacgtcagag tagatcacat cccgaaagag gccgtgttcg gggcaggtct tctccatgat
     6421 gaccttgccg tcttcctcga acaggacggc ctcgatcttg ccagtgcact ccgggcagag
     6481 cgagatagtc agcttcggaa ggcccttggg gaccgacggg attgccgagc cggtgtaggt
     6541 ggttctcggt tggatgactt ggaactccgc tgctttgccg ggtgcgaatg tggcagtact
     6601 cacgatcggt tcctccacca gcatttcggg gctgaatctc ctgttttcag gtcaactcca
     6661 gtctaatcat tcccttgcag gcgcactacc ggtttaagtc aaacggtttc aggggccgtg
     6721 cggacggcga tttccggggg ttgaacggtc acgggcggct gcgaactgca gcccgcccct
     6781 ccccaagaaa aagaaccgcc ggggtgttgc caccccggcg gttgcgggac tggcggcttt
     6841 ccgtccgcct agtcggtaaa cacttcttca cgctcctgct cggcctgctt cgccgtgtgg
     6901 ccatttccgt tgccgttcag gctgttgatc tgcaccagcg gctccgcggg cttctccttg
     6961 agcacgacct cgcggtagag tttggccatc cgcgtgttgt gctcggcctc ctgccgcatc
     7021 ttttcgttgc gggcgcgagc cttctcctcg cgggcgttct tggcgatgcc cagccggtca
     7081 agatcgtgct gctcctcgcg ggtgatgatc tcctcgcgca gcgtcagcga gtgctccgtt
     7141 cgagctagtt cgaccctctt gccgccggcg aagatttcat gctttccgtg ctcgtcgtac
     7201 cacttggtca gcgtggccgt catgtggatg cgctccacga tgttgcgcca gccgacgccc
     7261 gtgttgtatg cgcagaacga gatctcgccc tcctgggtcg cgtacgggat gatgcactgc
     7321 tcggtgcgcc ggaagtcgta gttgaacaga tcctggaacc acatgccagc aatcatcagc
     7381 acgttccagc ggtctctccg gcggcgttcg atgtcctcca gcgtgcggtc cgctccaact
     7441 ttgccgtagc tcttgccggt ggcgccgaag gtcttgtcca ttttcttcag catatcggtg
     7501 agcttgaaat gtgtcggtgt ctggaagggg tcgtagttgc gagccacggc cagcgccatg
     7561 ccgaagatgg acagccacct gcctcgggcc gcgtcgttga tctgcatgac gtccttggca
     7621 agctggtcgg catttaaaaa agcagtgaac ggcaccgcct ccttcgtttc cttgtccacc
     7681 agcacggcca tgccgaggcc gcagttcggg tggcagccgc agctcaactg tccccagttt
     7741 gcctccggcc cgtgtacgag gtcgctccag tccgagaatg tgctcatgaa ggaaagcggg
     7801 aaccagtcgc gacgcggctc gccgatgccg gtctgcttct ttacgtcgta ggccaggtgg
     7861 gacagtgtgt atcgctgggc ggcgcggcgc tcattcgtca ccgcctcgtc gcgcccggtg
     7921 aacgaaaccg gctggaacga aaggaagggg atcttcttcg ggttatcgag cgcgaactgg
     7981 actacccggc ccacctgctc gtcgttgatg ccgttgacga tcgtgatcac gggcactatg
     8041 tccacgccgg ccttgtacag attctcgatc gcctggagct tcacgtcgaa caggttcccc
     8101 accttgcgat gcgagttggc tgcgttcccg atgccgtcaa actgaaggta tgcgtagcga
     8161 aggccggcct cggcggcctt cttgcagaat tcgacgctct tcgcgaattc gatgccgttc
     8221 gtggccgcct gtacgctcgt atagccgact tctcgcgcat aacggaccgc gtcgaggaaa
     8281 tagggggaca gcgtcggctc accgcccgag aactgcaccg acatctgccg cttcggcttg
     8341 atcgaaatcg cgttgtcgag cagcgtcttg atctcttccc aagtcagctc gtggacgaag
     8401 cccacctggt tggcgtccat aaagcacggg tcgcacatca tgttgcagcg attggtcagg
     8461 tcgatcgtca gcaccgctcc gcgtccgtgg gtcaccgtgc ttgacccgtg gtgatgcagc
     8521 ctctcgtcgt tgtgcgcccg gatgtcgcgg ccagggaaca ccgactcaat gtgcttcatg
     8581 aactcggcgt cgatggacat gatgtcctcg aaatggccgt gcttcggaca atccttcacc
     8641 atccagatct ggccgtcgcg ttcgatgatt tgggccttga tttcgcccgg cttgtcatgg
     8701 atcagcactt ccagcggctt cttgccatcc aggatctcct ggcggatctc cggaacgcac
     8761 ttcgggcaga gggaatccgt ctcccgtggc cacccaagcg gcggcttctg cttttcccac
     8821 gacttcagca ggggcttgtc ggaccacttc ggggtgaagg aggggttcct cccaatctgg
     8881 tttagcctcg agaatacggc ccaggcccca ctggccgtga gcgcgactgc tttttcgaag
     8941 tacttgatag gtttatgcat gttttctcgc ttcaggtgac tcccagtctt gacgccagtc
     9001 gttcctgaca gccctgtccc tctttccagg cagccaggcg atcattctga gtatagggaa
     9061 ggccaagggg tggaaccagc caaatgaatt aaactgcttc ttttctagct cgtatggtga
     9121 attctgaggc tgaatgggtc ggcatagccg gagtggagat tcaagtgcca gtagaactgc
     9181 tgctgtctcg ccgcaggtta gcatgaaaac agaggccatg cctgtcagcg ccaaacgagt
     9241 caaggagata gcggcggagt gcggttttga gatcgtcggc gtggcctcgg ccgcaccgtt
     9301 gtctgagcac gcctggtacc gtgagtgggc cgccctgggg atggcgggaa gaatgagtta
     9361 tcttactgac cggcggggag atgcgcgtca agatccccga aggctcctgg cgtcggcgcg
     9421 ctcggtgctt tgcgtgggca agctgtataa cgctccgaag ccctactccg ttgattttga
     9481 agctgttgag cttggctgga tctctcgtta tgcgtgggga gaggactatc accgcgtcgt
     9541 ggccgatggt cttcatcgag tcgtggcccg gctcaagtcc agtggcgccg ggcctttcga
     9601 ctggaaggtt tgtgtcgata ctgcgcccct tttggaacgg gccctggcaa ggcgcgccgg
     9661 actgggctgg atcgcgaaaa acacttgcct gatccgggaa ggaatgggtt cctggttctt
     9721 tctaggtgaa ctgctgctct cgctggacct tgaatccgac tctccgccgg acgaacgctg
     9781 cggctcttgc acgcgctgca tcgatgcctg tccgacgcgg gcgatcgttc cagcgggccg
     9841 cgccgagccc gcgtgggcca tcgattcccg cctgtgcatc tcgtacctga caatcgagct
     9901 gcggggtccc tggcctcacg aattgcgcgg ggctgccggc ctcaacgtct tcggctgcga
     9961 catctgccag gacgtctgtc cgtggaacac gaatgcgcct gtaacgccgg actctgcgtt
    10021 cgagcctgcg aacttcgccc cgcccctcga acggatggcg gcactcaccg aggaggagtt
    10081 cgtcgcgatg tttcgcggca cgccggtcac gcgggcgaag tacaccggtt tcctgcgcaa
    10141 cgtcgccgtg gccatgggga actcgggacg tccggagttt cgagcaccgc tcgagcgcct
    10201 cgcccagcat ccggaccccg cggtggccga tcacgccggc tgggcgctgg cgcatatcgg
    10261 acggataaaa tgataagaga aatgctgttg gtgttccttc tgctgctgtg ctcggtggtt
    10321 gcatcgccgg cgtcggaagc cacgctctcg aaaggcttcg accactttta taatctcgag
    10381 tacgacgagg cgatcgcgga gtttcgaaag ctgatcgacc gcaatcccga gtccgcgggg
    10441 gcgcgcaacc attacgccca ggcaatcctt taccgggaga tgttccgcgc cggcgcactg
    10501 gagagcgagc tggtcagcgg aacgaacccg tttctccggc gcgcgaagat gaacccatcg
    10561 gcggaggacc agcgccattt cgacgaatcg gtcgccaaag ccattgaact ctcggatgca
    10621 cggatcaaga aagacccgaa tgacagtcag gctctctacg cgctgggcgt cacctacggg
    10681 ctgcgtgcga attacaactt ccttgtacgc aaggcgtggt tcgacgcttt gcgggacgcc
    10741 acccgcgcgc gcaagttgca caatcgggtg actgagctgg atccgaagat cgtcgatgca
    10801 cgactcgtgg aaggcgtgca cgactacgta gtcggcagcc tgccctggca tgtgaagatg
    10861 ctcgggtttc tggtcggctt ccgcggtgac aaggaaggcg gcgttcgcct gctcgagcag
    10921 gtcgcgcagc acggcgacat caatcgggtg gatgcccaga ttcttctctg tgcgatttac
    10981 cggcgagaaa ggaatccgca gaaagccatc ccgatgttgc tgagtctgat cgaacggttc
    11041 ccgaggaact atctgctgcg tttcgagatc gcccagatgt acagcgacct tggtgacaaa
    11101 ccgaacgcac tggccgctct gcgccggatc gaggagctga aatcgggcgg ggcttcggga
    11161 ttctcacacc tgatgccgga gaagatcctg tacgctcggg gcacgatcca gttttggtac
    11221 aacgacctcg atcgagcgct cgaagacatg cggcgggtga cctcgagggc cggcgatctg
    11281 gacctgaaca ccggtacctt cgcgtggctc cgcctcggtc agatttacga cctcaaggga
    11341 cagcgggcgc aggcagttgc cgcttaccgg caggccatca acttggcgcc gcaatcggac
    11401 gccgcccgcc tttccacgcg ctatttatcg gaaccctacc gtagagacaa gaggaagagc
    11461 taagtccgtg gtctgccctg gggagtgagc gcgccggtta ttcgatgtta actttaaccg
    11521 gctgttcgat cagcttcggt tgtttttcgg tggtcagcac cattaggctg aaccgtccgg
    11581 ttttcagcga ggcgggaacc ttgatcttga tgctggtagc agcctgttcg gtgatctcaa
    11641 ccttcgtatc cgtcttaccg tccgtcaaga acacctgcgc gaccgtctcc ttgccgaggt
    11701 tctctcctgc gacgttcaga acgtcaccgg cctttccggt gtctggctcc acggcggtca
    11761 tccgcggaaa ggcgccctgc gcaaccaaca cagaagagat gacgaggaaa gcgattaggg
    11821 cgagcgagat aaaagacaac ctcatcttgt tattgtcctc cacaaaaatg ctagccctaa
    11881 atcgctcgta atgcaagcgt taagcaaggt ggtagtgccc tgcaaccact acaggctgtc
    11941 ccgcctggca aaggcaaccg gacgccaacc gtaacatcgg agagtgggcc tatgacgaaa
    12001 ctgagcgcgg gttttctcat gttccggggg tcacaagggg cagtcgaggt tctcctcgtg
    12061 caccctggcg gtccattttg ggaaaagaag gacctcggat cgtggtcgct gcccaaaggc
    12121 gagtatcccg aaggcgagga ggccttcgat gctgcaatgc gcgagttcga ggaagagacc
    12181 ggcattcgtc ccgagggacc attcattcct ctcggtcaga ttcgccaacc aggcggcaag
    12241 ctcgtgaccg tttgggcttt tgaaggcgat tgtgaccctg ccacgatcaa gagcaatctc
    12301 ttctcgatgg agtggcccaa ggggtcgggg aggatgcaag agtttcccga gattgaccga
    12361 ggcggctggt tcacgttgac agtggcgcga cggaagatcc tgaaggggca agtgggcttt
    12421 ctggaccggc tcgccgaaca tttggacgtg cggaaattat tcgtaccgta acgccaccac
    12481 gggggtgacc ctggtggcgc ggcgggcggg gaccaatgtg gcgatgagcg ccacagcggc
    12541 taacaccagc gcgacgccga tagaggtcac cgggtcgcct gcgttgacgc cgtaaagcat
    12601 accggccatg aggcgggtga gcccgaagga ggcggccacc ccgatggcca ggccggccaa
    12661 aacgggaaac atgccctggg cgaggacaag cttcagcaca tcggcctgac cagccccaag
    12721 cgccatgcgg atgccaattt cgcgtgtacg ctgcgcgata gtataagaga taaccccgta
    12781 gatcccgacc gcggccagca acagcgctag cccagcgaag atacccagga tcagtgtgga
    12841 caagcgccgg tccgcgatcg aacccgagac gatctgttcc atggtccgca ctccgtagac
    12901 ggtctggtca ttgccggcct cgaggaccgc gctgcgtact gccgggaatg tggcgagcgg
    12961 atcgactcca gtgcgcacta cgaagacact ggatcctgcc atcgccttca tgaactggtc
    13021 cgggatctga gacaggggca ggtaaagctc gctgcggacc cgggcggagg cgtcactgtc
    13081 cagcccccag tggctgacgt ggccgacgat acccacgatc tccatcggca cggaagcctc
    13141 ggggccgaga tctccgctca agtcggcaac gacgatgctt tttccgagag ggtcttcgtg
    13201 cgggaagaac gtgcgagcca taacctcgtc gatcaccacc acgtgggagc cgcgatgcat
    13261 atcctggcgt tcgatgtaac ggccgcgcaa gaggggaatg tccatggcct tgagatagcc
    13321 ggggtcggta gcgtacaaca gcgcccacga catgtcgcct tgtgaggtcg ggcgaggcct
    13381 tccgttcaca tagaagggta tttcgctgtc gctgccgccc aggggaatca acgaggacac
    13441 cccggcgctc ttcactccgg gcacactttg gatacgatcc gcggtctgct cgaaagtctg
    13501 gaggatacga tcggtgctgc tcatgttggc tggggacaag cccactgaga aggtcagaac
    13561 gtggtcgggt ttgaatcccg gtttaatgct gccgaggttc cagatggtgc gcagcatcag
    13621 gccggctccg gccagcagca ccagcgccgc ggccacttca gagaccacca gcaggttgcg
    13681 caagcgctgg tgccgggccg aggaaccgcg gctgccttct ttcaacgtct cgtgcaggtc
    13741 ggcgacggag acgtgcaggg ctggagccag cccgaaaacg atgccggtaa ggagcgagac
    13801 gcccagagta aaggcgagca cccagccgtc cacgccgatg ttctccacgc gcggtaggcc
    13861 gcccggcacg gccgccaaca ccgcctgtgt gccccagcgg gcgatggcca gcccggcgat
    13921 gcccccggcg agcgccagca ccacgctctc ggtaagcagt tgccggacca cgcggacgcg
    13981 gccggcgcca agcgcggagc ggatggcaat ctccttgtgg cgtctggtga agcgagccag
    14041 gagcaggttc gccacattgg cgcatgcgat gaggagcacg aagcccaccg cgcccagcag
    14101 aaccagcagg gtggggcgaa cgtttccgac gatcacgccc gtgagcggct cgatgttgac
    14161 gccgtgcttg gaattcgatt tcggataagc ttccgctaac cgggccgcaa tggcggtcat
    14221 ttcggactgc gcctgggtcc gcgtcacgcc ggctttgagc cgtcccacca cgtacatgcc
    14281 gggatgcatt tcgcgcgacc tggccagcat atcgtcccac tgttccagca gcgtgtagat
    14341 gtcggctttg ccttcgaact gaaaaccggc gggaacgacg cccgcgatgg tgtagctccg
    14401 gccgttgagc gtgagctggc tggcgagtac ggcggggctt gaaccgaacc gccggctcca
    14461 caggccgccg ccgatgagga ctacgggcga ggcgccgagc cggtccgccc gggggtcgaa
    14521 gtcgcggccc gccacgggat taattccaag gacccggaag aaatcggccg agaccatctt
    14581 gccgctcagg tgctcgggct ctcctccgcc ggtgatgtcg tagtcttccc agcggaacgc
    14641 ggccaagccc gcaaagctgc ggttctggtc gttccagtcc acgaagttcg gataggcgac
    14701 cgacatcgtt tcgaagccgg gcgacctctc gctgagccgc acgaggcggt ccggatcgcg
    14761 gtagggcagc ggctgcagca gaacgccgtt gaccacgctg aaaatggctg tgttggcgcc
    14821 aatcccgagc gccaaggcaa tcaccgctac cgccgcgaaa cccgggttct tggccagcat
    14881 acgcaagcca taacgaaggt cctggagaag tacccacatc gagctgcctc ctcacgctga
    14941 gccgtaccac cttgccttgg cgccttatcg atcagtacgg gcgcagccgg ggcgatgttc
    15001 ctggtaattt gctggatgca gaggggttca gactgcgcgg gcctggcgct tcttcgccgt
    15061 ggccgccatc agcttctcac gctcggcggc ttccttcgcg cggaacttcg cgatgcgtcc
    15121 gatcaacctc acggggacgg gctcggagag cgggaagcgg atcgtgccct tggtgacctc
    15181 gtacgatgca agctcgtcct tgaacgccgt gacgaggtga ccggtggcgg gatagagcga
    15241 gtagtgctgc ttccacccag cgaaatagat cgcgacgcga ccgtgcagtt tgtatgcggg
    15301 gatctggtac gagatcactt cttcggctcc gggcatcgcc ttgcggatcg tgcttcgcac
    15361 gcactcgagc acgctctgaa cagtttcagg ctgcgaggca atgtactcgt ccacggactt
    15421 ccaatcggtc ttggccatcg tgattcccag ctttccggct caggttgagc gccctctcgg
    15481 ccctgaatta gcagcagaat actacggatt atctcactca tcaatttcgt tgccaatgcg
    15541 tcaaagggag ctggctgatt tcgctcgtcg tggtggattt cggccacctg acggcgcggt
    15601 ctgccaacgg gaatcgcttt ggaatcatag cgtttcgtcg ttggcttcag aagtgctcca
    15661 ttctcggtgt gatggaaatc aagccgaaag gagaacccgt atgaaagcga agatggccgt
    15721 tctcattctg ctgctggccg caacctctgc gttggcaagg actcgcgtgt ccattggagt
    15781 ctacgtgggc gacggttacg gttacgggta ctatcctccg ccgccggtct acgtttacga
    15841 gccgccctgc ccagggccgg gttacgtatg ggttccgggg tattggtatg gcgtcgggcc
    15901 gcgctattcc tggcgcaacg gctactgggc gccgcctcgt taccgcggct ggtatagggg
    15961 tggaccaagg tatcgctatt acgacgcatg ggatagcccg taccgtggac ggatgaagta
    16021 tcgcaaacat gacgatcgcg gctgggggcg cgggcgctac cgtgactatt accggtacga
    16081 tcggcgcgga tatcgccggt gaatgagcac gatactcgtg cgcgactacg gttggtggct
    16141 caccaccgcc atcgagtaga gtcccatcga cgggacctgg aagactaccc tgccggcttc
    16201 ctccttaaac tgggcggaaa tcggctcgtc cgaatccggc gagaagacct tcacgcttga
    16261 gaccttgccc gggaactgct tccgcaatcg gacctcaaag gagtccaggc gctttccctg
    16321 ctcgaagttc acgaaatgca cgatcgtctc gctggacttc ttgcggttca gcaactccat
    16381 caccgtgttc agcggcgcag cggatgcgat cgaaaggccg ccgcgagcat tgtccgcaat
    16441 cgcgtgatgg atctcctcgt gattgggagg cagtaaccac tgggatgccg gaaagtcggc
    16501 gcgtccgcca gtggcgactt cgacttcggt ctcgtcaccc gcggcagtgg cgcggccgcg
    16561 cgcttggcgc cccacttcgg ggatgcagac gaccttgccc tggccgacgg tcttcacggt
    16621 gattgcagcg ggacgcgccg gggctcccat caaagccagc agagggttgg cggctcgccg
    16681 ctcgcgtctc tcgttgaagt cggccgtgtt gccggtgaaa accagcgtgc ctccaccgcg
    16741 cacataggcc gacaatcggt ctacccactc ctgagacagg ctctcctggc ctggcaggat
    16801 caccgcgcgg tagcggtcga tgcggtcgat ctggccgtcg aagaggatgt cgaacggaac
    16861 cttgtgctgg atgaggacct gctcgataag aatcgtcggt acgagggtcg cggctacgct
    16921 gtacgccatg gacggccacg tgcgcacgac cgcaacgtcg gcgacgttct ccgtctccgt
    16981 gtagtagcgg tcattgtact cgcggaagaa ctcggcctcg ggcgagaacg cccgtgccgc
    17041 gccccagccc gacggtccac cttcgtagcc gtaccccggc acgtatttct gcggattgaa
    17101 agccatacac agcgcgaact cgagagcgtt gcccccctcc tgatagctga agttcagcgt
    17161 acgcgccatc ttgtaggagc ggatctcgga aaccatggcg ccggttctcg aatcgagccg
    17221 cgcgtccatg cccccgacgt cgaacggcga gaagtcgatc ttgccttcga agaaggggtg
    17281 atacacggcg ttccgccaga tgcggttcac cccgtagatt cctttcagat tgaagccaac
    17341 cgccagcttc ggattggcgc ttttcagaaa gtcgtacagt tcggcacaat ggcgcgccaa
    17401 cgcg
//
LOCUS       DATIFQ010000454        16209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_727314, whole genome shotgun sequence.
ACCESSION   DATIFQ010000454 DATIFQ010000000
VERSION     DATIFQ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16209
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727314"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VLE22_22820"
     CDS             complement(<1..631)
                     /locus_tag="VLE22_22820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17302.1"
                     /translation="MRSSVAVLLLAACGAWAQEAAFDSRTSLKIDFPKDSPVAVVSAD
                     WDGGESRVSARGSAMVLDLRTSLMLRNSGSQRIRGVSLLVLAQEVSPGGRASVTVPSL
                     DVGPGDTFPVRIDLRLLRPLLVGGGPMVQVSLDGVLYSDLSFFGPNRLNSRRTMTVWE
                     MEARRDRLHFKSVLTTQGADALQKTVLRSLARLDERPRLDVRVSRGRSTN"
     gene            complement(621..1187)
                     /locus_tag="VLE22_22825"
     CDS             complement(621..1187)
                     /locus_tag="VLE22_22825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17303.1"
                     /translation="MRHPNETDLALYAGRELGWFERRRVARHLTECALCRREAEAFGA
                     ARAELAASAVEIPAEVNWGRLAAEMRANIHVGLAAGECVGSAEVEPWPFFRRAAATVV
                     PVALLLLAGWWLQSDRPHPAEMAAEEGVVLRATSEGIELREGGRLLSLQNHDSQDVTY
                     TVSMQGAIRARYVDSETGQVTINHVYAQ"
     gene            complement(1184..1753)
                     /locus_tag="VLE22_22830"
     CDS             complement(1184..1753)
                     /locus_tag="VLE22_22830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB17304.1"
                     /translation="MVTVLEDLRIAMSEAAEPTIHEGAERQNRFIAENLRRVFLMIYR
                     MVGNVADAQDLTQEVFIKCLQRGGQLRDAEKAAHWLSRIATNTAIDFLRRNRRASFTE
                     IDEVAEPLTSQFDSPEQSVLRSERRAYIDDGLRLLTERERLALLLRDVDGLPAEEVAR
                     QLGCSKATVRSHIANARIKFRRYVERRRS"
     gene            1919..3082
                     /locus_tag="VLE22_22835"
     CDS             1919..3082
                     /locus_tag="VLE22_22835"
                     /inference="COORDINATES: protein motif:HMM:NF004720.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HSB17305.1"
                     /translation="MRPVAVVGIGKTAFGAFPDRDLRGLTVEAARKCLENGHIAPSQV
                     EAFYLGNFAGPAFAGQNHLAPYIAASMGIVDVPATRFEAACASGGSAFFHAVSAVAAG
                     IYDLVLVAGVEKMTSQPTPKVAEILASAGDLGGEIRVGGTFPALFAMIARRHMHEYGT
                     TREQLASVAVKNHANGAKNPLAHMRKVITLEQALAGKPVADPLTVYDCSLVSDGAAAV
                     LLAPLERASEFTDKPVRILGIAQTSDHLALEEKADITTFPAIRRAAEKAYRMAGVTPD
                     DIEFAEVHDCFTIAEIVAIEDLGFVKKGEGGSYSAAGCTRPDGPKPVNASGGLKSKGH
                     PVGATGVGQICDVALQIRGEAGDLQLSRHALGLAQNLGGSGATGVVTILGAAA"
     gene            3079..3312
                     /locus_tag="VLE22_22840"
     CDS             3079..3312
                     /locus_tag="VLE22_22840"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HSB17306.1"
                     /translation="MIEERKLGPGIVYTETVVYSAPEAFVNDAPYQIAIIALDAGGRI
                     TARIEAERVSIGDRVRFAGYRNGIPFFHKHLEE"
     gene            3392..4552
                     /locus_tag="VLE22_22845"
     CDS             3392..4552
                     /locus_tag="VLE22_22845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSB17307.1"
                     /translation="MRLAERMNRIGTEGAFQVLARARQLESQGHSVIHLEIGQPDFET
                     PRHIIEAAKLALDDGWTHYGPTQGYPELREAIAAYICRTRGISVGPDHVAVVPGGKPV
                     IFFPMLALIEPGDEVIYPNPGFPIYESMVRFLGGVPVPIPLVEERGFSFDLDRLRDSL
                     SDKTRMLILNSPQNPTGGVILGEDIATIAEMVRDRDLIVLSDEIYSRIYYGQPPVSIT
                     TLPGMLEKTIILDGFSKTYSMTGWRLGYGVMPLWLVDAVNKLMVNSNSCTASFTQRAG
                     IVALNGPQLEVERMVAEFRRRRDAFCAGLNRLPGVRCALPGGAFYAFANIRGTGFSSR
                     ELADRLLAEAGVACLSGDSFGAFGEGYVRFSYANSYENLMEAVERIERFLAA"
     gene            4659..5006
                     /locus_tag="VLE22_22850"
     CDS             4659..5006
                     /locus_tag="VLE22_22850"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSB17308.1"
                     /translation="MYSPNPLTPAVFHILLAIADGERHGYGIMQEVSVRTGGKVVLGP
                     GTLYGTINRMLEAGLIEESGERSDPELNDERRRYYRITGKGWRAAVAEAERLQGLLRL
                     ARAKHLLQRPRPV"
     gene            5016..5612
                     /locus_tag="VLE22_22855"
     CDS             5016..5612
                     /locus_tag="VLE22_22855"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HSB17309.1"
                     /translation="MSFSEKMYRFLLRAYPRDYRIRYAEPMEQLFRDRLHEVHALADL
                     VALWGRTLADWAVSVPARYLERVTPHTRSGSLADPARRCIFFARSEASSFSRREITVE
                     HLLLGVLREEPSLVPGMKLEAVVRTIEANEPVGRRVPPMEDLRLSEETIRVVAAAREI
                     ARAAGRRQVAPVDLATGILREADTLAARILREHISDRA"
     gene            complement(5764..6693)
                     /locus_tag="VLE22_22860"
     CDS             complement(5764..6693)
                     /locus_tag="VLE22_22860"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB17310.1"
                     /translation="MTRRIFLMAGGAALASGRGAMPAATKLKVSVFSKHLQFLKDEAL
                     AETAATLGFDGVDLTVRPGGHIEPERAEEELPRLVKILRARRLAVEMVTSGIVDADTP
                     HAESVIRTLAELGVRYYRWGGFLYEPDRPIPAQLDALKPRVAKLAALNEKYKVCAMYH
                     THSGLGQVGASIWDLYILLKDFNPDAVSVNYDTAHATVEGGYGGWINSLRVTETHLRG
                     VAVKDFLWEKDSKGNWTPEWCPVGEGMVRFPQFFRMIAKRKFAGPLQMHYEYPLGGAD
                     TGKTTITIPREQVYTAMKRDLKLVRGWLAEVGL"
     gene            complement(6805..7041)
                     /locus_tag="VLE22_22865"
     CDS             complement(6805..7041)
                     /locus_tag="VLE22_22865"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HSB17311.1"
                     /translation="MGNTLEELKAHLIETNEEFRRLATQHSEYKQKLKELADRHYLTE
                     EEQVEEVRLKKMKLRLKDQMERILGESRRTENVA"
     gene            complement(7177..7650)
                     /gene="rimI"
                     /locus_tag="VLE22_22870"
     CDS             complement(7177..7650)
                     /gene="rimI"
                     /locus_tag="VLE22_22870"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HSB17312.1"
                     /translation="MKRWWPWKGDRTGRRPSFGHVAIRPASQADLPEIARIQTASPEA
                     ARWRPESYLPYDCLVATLDGCVAGFVVARQTAPGETEILNLAVAPEARRGGVGTALMH
                     EAMARRSGEIFLEVRQSNEGARALYRRLGFQEAGIRQRYYIDPPEDGIVMRLQSC"
     gene            complement(7647..8834)
                     /locus_tag="VLE22_22875"
     CDS             complement(7647..8834)
                     /locus_tag="VLE22_22875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB17313.1"
                     /translation="MRFRVPVRWAVMGVFVLSAALNYLDRQVLAALAPLLRDEFSLTN
                     AQYGQVLAAFSITYAVCAPLAGLLIDRFGLNRGICVGVGLWSLAGVATGAANSIGGLF
                     ACRAALGVGESAGVPATGKALHLYLPARERALGNALSQIGLSAGAIVAPPLATWVALR
                     HGWRSAFVVTGVLGFIWIPLWLWASRKTAAISEPSNAPVSPTGMLRDRRMLGFIVATF
                     LSMTVYTLWTNWTTLYIKETYGTTLTKANWLAPLPYLFAYAGGMFGGWLSLRWIDAGM
                     EALAARRRACLVSAVALLGTAAVPLMPTAVWAAFGIAFSFFAISAWSVNLYTMPLDAF
                     GMERAAFGMAVLTSAYGAMQTVISPLLGATIDRYGYPPVLVAVSLLPLAAWSILQGTR
                     RRA"
     gene            complement(8831..10531)
                     /locus_tag="VLE22_22880"
     CDS             complement(8831..10531)
                     /locus_tag="VLE22_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17314.1"
                     /translation="MLRLTVLLIFAVLACAQDRWVEYRFGPFHVVTNAGEREGRDTLA
                     QLTQFRHALGRLTGKTDVVSTWPIRILLFKAAKQVPASAGGLSLGRDAYTGALAARQP
                     LPRPMLRDCARTLLESNVGRLPRDIERGIVELLSTLETDGLRITIGQPPQEADRTRDW
                     AKLHMLTVPENYYGKLRVLVHNLEQGADPEPAYRNAFGKSPVAIEKEVDAYIRAASYS
                     TTSVNGLTLNPLKDFATQPVDASVVDLYLADLYLGRTETNAQARGAFEAVLKRTPASA
                     EAAAGLGLAALAAGDANEARKALGAAVAGGTTSARALAEYGRLEADDAKALAALEKAA
                     KLNPRWAEPYALMARREQNKQRRLQYLATAAKLSPRDAGAWQALALAYQAAGKIPEAA
                     DAWASAERAAGDEERGKIREARAEIDRKRIEFEAAEKKREEEEKRRELEELKQKSVAS
                     IQAALDKANRGDTPPAPGGKVEPWWEGPQADAKITGVLRQVDCLGRRVRLVIEGADKR
                     PVRLLIRDPGKVVIQGGGDRTLGCGPQRPPRRVTVEYFTKPDAKLGTAGEAAVIDFPQ
                     "
     gene            11027..11971
                     /locus_tag="VLE22_22885"
     CDS             11027..11971
                     /locus_tag="VLE22_22885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17315.1"
                     /translation="MTILTRLQQWKESGIISSEQHTLLAGLSLGEPFSIFLELNILLY
                     AGILAFVAGLGWTVSTWSQQLGDVVVLTILSAMLAASFWYCFSRAAAWSPGETPPPGL
                     VFDYVLYLGSLIWSVELSYFETRFHLLSGHWDLYLLATAGLFFFLAYRFDNRFVLSLA
                     LSALAGWFGLTISHWPSHQDAAYRQYAILYSLMVGGGGAALQRGGLKPHFFGTYLNIA
                     ANVLFWALLSGVFNREGYVVWLLCLLTACGASLAWGLKRRQFSFVAYAAVYGYVGVSA
                     VLIRDMHEDTAVLGYFVFTGVAMLVMLVQIARRFGREG"
     gene            11968..13176
                     /locus_tag="VLE22_22890"
     CDS             11968..13176
                     /locus_tag="VLE22_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17316.1"
                     /translation="MRAYSASSEEARRARDLLQDWWGEGLLTVEQYLRMEQETVCELR
                     RTNIFLRLVLLLFTLIILGAAVGLFFALFFSHPGEQTLGIFLLIFAALSYAAAEIAVT
                     KAQLYRFGIEEALAACSVGFLCVGMQLALFSGLFSSPTPARTGFLVPAAGAAVSLWMW
                     HRFGLPYASLGAMIFVAWLPSYWTSSPSGQHLIVAAVYSAGVVMVAAVRPRYRFTYLD
                     SEYSIAEALLWLGIYLAVNLQLSSVDLRGYWWSGSRSTAQFSRLFYWATWVLIWCLPP
                     VVLARGLRRKDRFVIAVGALAAILTLVTNKPYLGWQRHTWDPMLLGAFLIGVVLLIRR
                     WLAKAPGGIHHGFTAQRLSGKDKRWMNVGVATIGLASPNIVTPRGQTGGPDVHFGGGD
                     SGGGGATSDF"
     gene            13638..15665
                     /locus_tag="VLE22_22895"
     CDS             13638..15665
                     /locus_tag="VLE22_22895"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HSB17317.1"
                     /translation="MKRSVALFFFLLYVCPAQVVFQRADRGGAIIEIDASRKAGYTIP
                     RTIYGTFLEPIGKSIYGGLWAQILENPSFEENLWNAGNIRRMIDREPALERASAIGLP
                     LPWEPFDPAQGSRYEPRWGDAANSSRSLFIMALPDKQTGVRQEVYLPVHRTLRYTGSF
                     HARHFSGPAEVGVSLRRRNHPETVFARAALQLAGSAWARYEFTLELTRGQVAALEPAD
                     FVIALRDEGRALVDQVFLFPADHVNGMDPDMIALSRALKTPLVRYGGNFTSGYHWRDG
                     VGPIDRRVSMLNQSWGMPEYNHFGTDEFLEFCRLIGADPQIALNLGSGTPDEAAEWVH
                     YVNDRWGDRRGGLLWELGNELWGTFQIGYPTLERVAGRTRAFAQAVRRADPRARLIAT
                     GADPDHFSEWNAAQLGLPPGSFDFLATHFVVGAGGVRRSNPSSEFVTLSAFALPIGLE
                     RKLREMKEQIDAVPEWRGRTKIAFTEWLFHGPDERVPRFSNMGGALCAAGFLNSLMRV
                     AGFTPVSDMTGLIEFGGIWKKRGRVYGVPAYWAFRMYSNTDASVPVSTRVTVEHYDIE
                     QGNNRIPDIRDVPYLDVVAALNQAGDRLTLFCVNRHLYRDLPAAIRLSGFAPSGRASV
                     KTLAGTSIHQPNDEMRPEAVHPVDSVAPAAPEFEYTFPRSSVTVLEMTAGR"
     gene            15896..>16209
                     /locus_tag="VLE22_22900"
     CDS             15896..>16209
                     /locus_tag="VLE22_22900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17318.1"
                     /translation="MKVSVEISRGIESLFGTHDENIRLLETGLNVSTQLLEDCLEIEG
                     EEANVARAERILEDYVSLVREGHTFANGDLNSYLRVVTEDPGVRLRDLVLSGRQRSFG
                     KKV"
BASE COUNT         2761 a         5507 c         4789 g         3152 t
ORIGIN      
        1 cgttggtcga gcgtccccgt gacacccgca cgtccagtcg cgggcgctcg tccagccgcg
       61 cgagactccg caggaccgtc ttctgcaacg cgtccgcgcc ctgcgtcgtc agtacggact
      121 tgaaatgcag ccggtcgcgc cgcgcctcca tctcccagac ggtcattgtc cgccgcgagt
      181 tcagccggtt cgggccgaag aacgacaggt cgctgtacag caccccatca aggctcacct
      241 gcaccatcgg accgccgccc accagcagtg ggcgcagcag ccgaaggtca atccgtaccg
      301 ggaacgtgtc gcccggcccg acgtccaggc ttggcaccgt caccgaagcc cggccgcccg
      361 gcgacacctc ctgcgccagc accagcagcg acacgccgcg gatccgctgc gatcccgaat
      421 tccgcagcat cagcgacgtt cgcaggtcga gcaccatcgc gctcccgcgc gccgaaaccc
      481 gcgattcccc tccatcccag tccgccgata caacggccac cggggaatcc ttcgggaagt
      541 cgatcttcag cgatgtgcgc gaatcgaacg ccgcttcctg cgcccacgcc ccacaggcgg
      601 ccagaagaag gacggccacg ctactgcgca tacacatggt tgatcgtcac ctgacccgtt
      661 tccgagtcta cgtaccgcgc gcggatcgct ccctgcatgc tcaccgtgta ggtcacgtcc
      721 tgagaatcgt gattctgaag gctcagcagc cggccgcctt cccgcagttc gataccctcg
      781 gaggtggctc gcagaactac gccctcctcc gcggccatct ccgccggatg cgggcggtcc
      841 gattgcagcc accatcccgc gagaagcagc agcgccacgg gtaccacggt cgccgccgcg
      901 cgccggaaga acggccacgg ctccacttcc gccgaaccca cgcattcgcc ggctgccagg
      961 ccgacgtgga tgtttgccct catttccgcc gccagccgtc cccaattcac ttccgcggga
     1021 atttcgaccg ccgatgcggc cagttccgcg cgcgccgcgc cgaacgcttc cgcctcccgg
     1081 cggcagaggg cgcactccgt gagatgccgc gccacccgcc ggcgctcgaa ccagccgagt
     1141 tcacggcccg cgtacagcgc caggtccgtc tcgttcggat gtctcatgac cttcgcctct
     1201 ccacgtaccg gcggaacttg atccgcgcgt tggcgatgtg cgaacgcacc gtcgccttcg
     1261 aacaacccaa ttgcctcgcc acttcctcgg ccggaagacc gtccacgtcc cggagcagca
     1321 acgccagccg ctcgcgctcc gtcagcagcc gcaatccgtc gtcgatatag gcgcgccgct
     1381 ccgaccgcag caccgattgc tccggcgaat cgaactgact ggtcaacggc tccgccactt
     1441 catcaatctc agtgaagctt gccctgcggt tccgccgaag aaagtcgatc gcagtgttgg
     1501 tggcaatgcg ggaaagccag tgggccgcct tctcggcatc ccgcaactgg cccccgcgct
     1561 ggaggcactt gataaagact tcctgcgtca ggtcctgcgc gtcggccaca ttgcccacca
     1621 tccggtagat catcaggaaa acgcggcgca ggttttccgc aataaaccgg ttctgtctct
     1681 ccgcgccttc gtggatcgtg ggctcggcgg cttcgctcat ggcaatccgc aagtcttcga
     1741 gcaccgtgac catacgccta ttagggatga cgcgaaacca cacgaaacgt ggaggtcatc
     1801 tccctgcgtt cggtcccggt gacaagggcg caatgatgcc tctttgccct tcctgggccc
     1861 ctcggcccgc actcgcccca gcctcggagt agaatggctg aggcgctact ctgagtatat
     1921 gagacccgtc gccgttgttg ggataggaaa aaccgcattc ggcgcctttc ccgatcgtga
     1981 cctccgtggt ctgactgtcg aggccgcccg taagtgtctg gaaaacgggc atatcgcccc
     2041 gtcccaggtc gaggccttct atctaggtaa cttcgccggg ccggccttcg ccgggcagaa
     2101 ccatctggct ccctacatcg ccgcttcgat gggcatcgtc gatgtcccgg ccacgcgctt
     2161 cgaggctgcc tgcgcctcgg gtgggtcggc gttcttccac gccgtctccg ccgtggccgc
     2221 cgggatctac gatctcgtcc tcgtcgccgg cgtcgaaaag atgaccagcc agcccacgcc
     2281 caaagtggcg gaaatcctcg cctcggcggg agacctcggc ggtgagatcc gcgtcggcgg
     2341 taccttcccg gcgcttttcg cgatgatcgc ccggcgccac atgcacgagt acggcaccac
     2401 ccgcgaacag ctcgcctcgg tggcggtgaa gaaccacgcc aatggcgcga aaaacccgct
     2461 cgcgcatatg cgcaaggtga tcacccttga acaggccctc gccggaaagc cggtcgccga
     2521 cccgcttacc gtctacgatt gctccttggt cagcgacggc gccgccgctg tgctgctagc
     2581 gccgctggag cgcgcctccg agttcaccga caaacctgtt cgcatcctcg gcatcgccca
     2641 aacctcggat catctcgccc tcgaggagaa ggccgacatc acgacgtttc ccgccatccg
     2701 ccgcgccgcc gagaaagcgt accggatggc cggcgtcact ccggacgaca tcgaattcgc
     2761 cgaagtccac gactgtttca ccatcgccga aatcgtcgcc atcgaggacc tcggattcgt
     2821 gaaaaagggc gaaggcgggt cgtattcggc cgccggctgc acccgcccgg atggcccaaa
     2881 acccgtcaac gccagcggcg gcctcaagtc caagggacac cccgtcggcg ccaccggagt
     2941 cggccagatt tgcgacgtgg ccctccagat ccgcggcgaa gccggcgacc ttcagctttc
     3001 gcgccacgcg ctcggccttg cgcagaacct cgggggttcg ggcgccaccg gcgtcgtcac
     3061 catcctggga gcggcggcat gatcgaagaa cggaagctcg gccccggtat cgtctacaca
     3121 gagactgtcg tctactccgc gccggaggcc tttgtgaacg acgcgccgta ccagatcgcc
     3181 atcatcgcgc ttgacgccgg cggccggatc accgcccgca tcgaagctga gcgagtgtcc
     3241 atcggcgacc gcgtccgctt cgccggatac cgcaacggca ttcccttctt tcataagcac
     3301 ttggaggagt gacgatagca gcacgaaaaa gtggtgctct tagtgccttg ctgcgctata
     3361 ctctctttcg gtgcctggag gccgaagctc aatgagactc gccgagcgca tgaaccgcat
     3421 cgggacggag ggtgccttcc aagtgctcgc ccgtgcccgc caactggaat cccagggcca
     3481 ttccgtcatt catctggaga tcggccagcc cgattttgag accccgcgcc acatcatcga
     3541 ggcagccaag cttgcgctcg acgatggctg gacgcactac ggccccacgc agggctaccc
     3601 ggagttgcgt gaggccatcg cggcatacat ctgccgcacg cgcgggataa gcgtcggtcc
     3661 cgatcatgtg gccgtcgttc ccggcggaaa gcccgtcatc ttctttccga tgctcgcgct
     3721 cattgagccc ggcgatgagg tgatctaccc caaccctggc ttcccgattt acgagtcgat
     3781 ggtccgcttc ctcggaggcg tgcccgtacc gatcccgctc gtcgaggagc gcggtttctc
     3841 ctttgacctc gaccgcctgc gcgacagcct ctcggacaaa acgaggatgc tgatcctcaa
     3901 ctcgccccaa aaccccaccg gcggcgtgat cctgggtgag gacatcgcca ccatcgccga
     3961 aatggtccgg gaccgagacc tgatcgtgct ctccgacgaa atctactcgc gcatctacta
     4021 cgggcagccg cccgtgtcga tcaccactct gcccggaatg ctcgagaaaa ccatcatcct
     4081 cgacggcttc tcgaagactt actccatgac cggatggcgg ctcggttatg gggtgatgcc
     4141 tctctggctg gtggatgcgg tcaacaagct gatggtgaat tcgaactcct gcaccgcgag
     4201 cttcacccag cgcgccggca tcgttgcgtt gaacgggccg caacttgaag tcgagcgcat
     4261 ggtggccgag ttccggcgcc gccgtgacgc cttctgcgcg ggccttaatc ggctgcccgg
     4321 agtccgctgc gccttaccgg gcggagcgtt ctatgcgttc gcgaacattc gcggaaccgg
     4381 cttcagttcc cgcgagctgg ccgaccgcct gctcgccgaa gccggcgtcg cctgcctcag
     4441 cggcgactcg ttcggcgcct tcggcgaggg ctacgtccgc ttcagctacg ccaactcgta
     4501 cgagaacctg atggaggccg tcgagcggat cgagcgcttc ctcgcggcgt gaatgcacag
     4561 gagcgcgttg aggaaccgcg agtgtgaacg agcagcttca ggtcgcggtt cgtcgccgat
     4621 gacggaagcg gtcgatcatg ctagtatatc cgtgacggat gtattcgcca aatccactga
     4681 cgccggcggt gttccatatc ctgctagcta ttgccgacgg agagaggcac ggttacggga
     4741 tcatgcagga agtgtcggtg agaactggcg ggaaggtcgt tctgggaccg ggcacgctct
     4801 acggaacgat caaccgcatg ttggaagccg gcttgattga ggagtcgggc gaacggagtg
     4861 atccggaact gaacgacgaa aggcgtcggt attatcggat cacgggcaaa ggctggcgcg
     4921 ccgccgttgc agaggccgag cgccttcagg gcttgctacg cctggcgcgt gccaagcacc
     4981 tgttgcagcg gccacggcct gtttgaggac gatctgtgag cttctcagag aagatgtatc
     5041 gttttctgct ccgggcgtat ccgcgcgatt accgcattcg gtacgcggag cccatggagc
     5101 aattatttcg agaccgcctg cacgaggtac acgcgctcgc ggatctggta gcgctttggg
     5161 ggcggacgct ggcggactgg gcggtttcgg tgccggcgcg ctatttggaa cgggtcacac
     5221 cgcatacgcg ctccggttcg ctggcggatc ccgcgcggcg atgcattttc tttgcgcgtt
     5281 ctgaagcgag ttcgttttcg cgccgcgaga tcacagtgga gcatttgtta ctaggcgttt
     5341 tgcgggagga accctcgctt gtccctggaa tgaagctgga agccgtggtg cgcaccatcg
     5401 aggcgaacga acctgtgggc cggcgcgtcc caccaatgga ggacttacgg ctcagcgagg
     5461 agaccatccg agtggtcgca gccgcgaggg agatcgcgcg tgccgcgggg cgacggcagg
     5521 tcgcgccggt agatctggcg acaggaattc tgcgcgaagc ggacactttg gcggcgcgta
     5581 ttttgcgtga acacatttcc gatcgcgctt gaagccgctt ttgccgccga gggcatcgac
     5641 taatgcctcc aggtcgagag cctcaatccc tggtgatttg atgagaattc taaattgaaa
     5701 gtttagaatt ctcaagtact ggcactgccg tcagacttca caccggaggg tccttcgggc
     5761 gcgctacagg cccacttccg ccagccagcc gcgcaccagt ttcaggtcgc gcttcatcgc
     5821 ggtgtacacc tgctcccgcg ggatggtgat cgtcgtcttg ccggtgtccg cgccgcccaa
     5881 gggatactcg tagtgcatct ggagcgggcc cgcgaacttc cgcttcgcga tcatccggaa
     5941 gaactgaggg aatcgcacca tgccctcgcc cacggggcac cattccggcg tccagttgcc
     6001 tttggaatcc ttctcccaca gaaaatcctt caccgcgacg ccgcgcagat gcgtctcggt
     6061 gacacgcagg ctgtttatcc acccgccgta gccgccttcg accgtcgcgt gcgcggtgtc
     6121 atagttcacc gaaactgcat ccgggttgaa gtccttcagc aggatgtaga gatcccagat
     6181 cgaggccccc acctgtccga gccctgaatg ggtgtggtac atcgcgcaaa ccttgtactt
     6241 ctcgttcagc gccgcgagct tcgcgacgcg cggcttcagc gcgtcaagct gcgccggaat
     6301 cgggcggtcc ggctcgtaga ggaacccacc ccagcgatag taccggacgc cgagttcggc
     6361 tagcgtgcgg atcacgctct cggcgtgggg cgtatccgcg tcgacgatgc cgctcgtcac
     6421 catctcaacg gccagcctcc gggcacgcag gatcttcacc agcctgggaa gctcctcctc
     6481 ggcgcgttcc ggctcgatgt gcccgccggg acgcacagtg aggtccactc cgtcaaagcc
     6541 gagtgtagcg gcggtctcgg ccagggcttc gtctttcaga aactgcaggt gcttggaaaa
     6601 gacagacacc ttgagctttg tcgctgccgg catggcccct cgcccgctgg cgagcgcggc
     6661 gcctccggcc atcaggaaga tgcggcgcgt catagcccta tagattctca aaaacccaag
     6721 aaaaagaaca gccgcctcct ccgggttctt cttcggagaa aggcggctgc tctggtacgg
     6781 tgcccttttg gcgcacctga cggctcaagc gacgttttcc gtgcgacgag actcgcccag
     6841 aatgcgctcc atttggtcct ttagccgcag cttcatcttt ttcaggcgga cttcctccac
     6901 ctgctcctct tcggtgaggt agtgcctgtc ggcaagttcc tttagtttct gcttatattc
     6961 ggagtgctgg gtggccagac gacgaaattc ttcgtttgtc tctatcaagt gcgccttgag
     7021 ttcttcaagg gtgtttccca ttcggtgtct cccctgtgag gttgtcacga cgagtcggat
     7081 accggagttc gtaggctcgg tgggtgaact tcacttcgct catgaaaatc gaatgcggcg
     7141 gccggtcgcc tacgctacgg ccgtcacact tggacattaa catgattgca accgcatgac
     7201 aatcccgtct tccggaggat cgatatagta cctttgcctg atgccggcct cctggaatcc
     7261 gagccggcgg taaagcgccc gggcgccctc gttcgactgc cggacctcca ggaagatctc
     7321 cccggaacgg cgtgccattg cctcgtgcat cagggctgtc cccacacctc cacgcctcgc
     7381 ctccggagcc accgccaggt tcaggatctc cgtttccccc ggcgctgtct gccgcgctac
     7441 cacgaacccc gccacacatc catcgagcgt cgccaccagg cagtcgtacg gcaggtagct
     7501 ctccggtctc caccgcgccg cctccggcga cgccgtctgg atgcgggcga tctccggcag
     7561 atcggcctgc gaggctggcc ggatcgccac atggccgaag gacggccgcc ggcccgtacg
     7621 gtcccccttc cacggccacc agcgtttcat gcccgtctcc ttgttccctg aaggatgctc
     7681 catgcggcca gcggaagcag ggagacggcc acgaggaccg gcgggtagcc gtaccggtct
     7741 atcgtcgccc cgagcagcgg ggagatcacc gtttgcatcg ctccgtaagc cgacgtaagc
     7801 acagccattc cgaaggccgc ccgctccatc ccgaaggcgt ccaggggcat cgtgtaaaga
     7861 ttaacgctcc aggctgaaat cgcaaaaaag ctgaacgcga tcccaaaagc ggcccagacc
     7921 gccgtcggca tcagcggtac cgccgccgtc cccagcagcg ccaccgcgct caccaggcac
     7981 gcccgccgcc gcgccgcgag cgcttccatt cccgcgtcga tccaccgcag cgacaaccag
     8041 ccgccgaaca tcccgcccgc atacgcgaac agatacggca gcggcgccag ccagttcgcc
     8101 tttgtcagcg tcgtgccgta ggtttccttg atgtacagcg tcgtccagtt cgtccagagt
     8161 gtgtaaacgg tcatgctcag gaatgtggcc acgatgaatc ctagcatcct tctgtcgcgc
     8221 agcattcctg ttgggctcac tggagcgttc gacggctcag aaatggccgc cgtctttcgc
     8281 gatgcccaca gccagagtgg aatccaaatg aagcccagca ctccggtgac cacgaacgcg
     8341 gacctccatc catgccgcaa cgccacccac gttgccagcg gcggagcgac gatcgcaccg
     8401 gcgctcaggc cgatctggct caacgcgttt cccagcgccc gctcgcgcgc cggcaggtag
     8461 agatgcagcg cttttcccgt cgcaggcacg ccggccgatt cccccacgcc aagcgccgcc
     8521 cggcaagcga acagcccgcc gatgctgttc gcggcgcccg tagcgacgcc cgccagggac
     8581 cacagcccca ctccgacaca gatgccacgg ttcagtccga accggtcgat cagcagcccc
     8641 gccagaggag cgcacactgc gtaggtgatc gagaacgccg ccagcacctg cccgtattgg
     8701 gcgttcgtca gggagaattc gtcgcgaagc agcggcgcga gcgcggcgag cacctgccgg
     8761 tccaggtagt taagcgccgc cgacaggaca aacacgccca tgacggccca gcgcaccggg
     8821 acgcgaaatc tcactggggg aaatcgatca cggcggcttc ccccgccgtc ccgagtttgg
     8881 cgtcgggctt cgtgaagtac tcgaccgtga ccctcctcgg tggacgctgc ggaccgcagc
     8941 ccagggtccg gtctccgccc ccctggatta ccacctttcc gggatcgcga atcaggagcc
     9001 ggaccggccg cttgtccgcg ccctcaatca ccaatcgcac ccgcctgcca agacagtcca
     9061 cctgccggag cacacccgta atcttcgcgt ccgcttgcgg gccttcccac cacggctcga
     9121 ccttcccgcc gggtgcgggc ggagtgtctc cgcggttggc cttgtcgaga gccgcctgaa
     9181 tggacgcgac cgacttctgc ttgagttcct ccagctccct ccgcttctct tcctcctccc
     9241 gtttcttctc ggcggcctcg aactcgatcc gcttgcgatc gatctccgcc cgcgcctcgc
     9301 ggatcttgcc gcgctcctcg tctcccgccg cccgctcggc cgacgcccac gcgtctgccg
     9361 cctcggggat ctttcccgct gcctggtacg cgagcgccag cgcctgccac gcgcccgcgt
     9421 ctcgcgggct gagtttggcc gctgtcgcga ggtactgaag tctccgctgc ttgttctgtt
     9481 cgcgccgcgc catcaacgcg tacggttcag cccaacgggg attcagcttc gccgcttttt
     9541 cgagcgcggc caatgccttc gcatcgtcgg cctccagccg gccgtactcc gccaacgctc
     9601 gtgcgcttgt cgttcccccc gccaccgcgg ccccgagcgc ctttcgcgcc tcgttggcat
     9661 cgcccgccgc aagcgccgca agccccagcc ccgccgccgc ttccgccgaa gccggcgtcc
     9721 gcttcaggac ggcctcgaag gcgcctcgcg cctgtgcgtt cgtttcggtc ctccccaggt
     9781 acaggtccgc cagatagagg tccaccaccg atgcgtcgac gggttgcgtc gcaaaatcct
     9841 tcagcggatt cagcgtgagc ccgttcaccg aagtggtgga gtaacttgcc gccctgatgt
     9901 acgcgtccac ttccttttcg atcgctaccg gagacttacc gaatgcgtta cggtacgccg
     9961 gttcggggtc tgccccttgt tcgagattgt ggacgagcac ccgcagcttg ccgtagtaat
    10021 tctccgggac ggtgagcata tgcagcttcg cccaatcccg ggtccggtcg gcctcctgcg
    10081 gaggctgccc aatcgtgatg cgcaaaccgt ctgtctcaag cgttgagagc agttcgacga
    10141 tgccacgttc gatgtccctc ggaagccgtc ccacgtttga ttcgagcagc gtccgcgcac
    10201 agtcccgcaa catcgggcgc ggcaacggct ggcgtgccgc gagggcgccg gtgtaagcgt
    10261 cgcggcccag ggagaggccg cccgctgagg ccgggacctg cttggctgct ttgaaaagca
    10321 gaatgcggat cggccatgtg gagacaacgt ccgtcttccc cgtgagccgg cccagtgcgt
    10381 gccggaattg cgtcagttgc gccagcgtgt cccggccctc gcgctcgccc gcgtttgtaa
    10441 ctacgtgaaa tgggccgaat cggtactcca cccagcggtc ctgtgcgcaa gcgagcaccg
    10501 cgaagataag aagaacagtc agccggagca tccctgctaa gagaataccg tctccactac
    10561 atccgtggct gcgccgtcac gatcaacgcc cgggtgtgct ccttcgtcag cccgcggtaa
    10621 ccgtgcggct gcgagtacga gcgacccctc cagaacgtag acgaactccg agctgttgtg
    10681 aaaatggggc gttacctctg ggcagattta cgggttggcg actacacgtc cgaatgacga
    10741 acttcccaga gcacaggagt tggtgtttgg gtagcgacac gtgtttgtcc gccgtagggg
    10801 acgtcagtca ctgacctgcg tcctccagcc gatcgccgga ctctgcataa tatggaactg
    10861 tggcattgcc acctccaaat cttgttagca tctgaattct gccttgatgc tttttgtcga
    10921 gcctatcgca gtgtacgatt ggtagattca tggaccgaag aaacggctcc cggatcgact
    10981 ggccggatcc ggccagtcgc cccacatcct gagaacgagg acttccatga ccatactcac
    11041 ccgattgcag caatggaagg agtccggcat catatcgtcg gaacagcaca cactgctggc
    11101 aggcctctcg cttggagaac ctttttctat ttttcttgag ctgaatattc tcctctacgc
    11161 tggtattctt gcgttcgtcg ccggcctcgg ctggaccgtc agcacctggt cgcagcaact
    11221 gggcgacgtc gtcgtgctga ccatcctctc cgcaatgctc gccgcctctt tttggtactg
    11281 cttctcccgc gcggccgctt ggtcccctgg ggaaacgccg ccgcccggtc tggtcttcga
    11341 ctacgtgctt tacctcggca gcctcatttg gtccgtggaa ctctcttact ttgagacgcg
    11401 gtttcatctg ctctccggac actgggatct ctatctgctg gcgaccgccg gcctgttctt
    11461 cttcctcgct tatcgttttg acaaccgctt cgtgttgtcg ctcgctttgt ccgcgctcgc
    11521 tggatggttc ggactcacca tctcgcactg gccgtcacat caggacgcag cgtaccggca
    11581 gtacgccatt ctttacagcc tgatggtcgg cggaggagga gcggcacttc agcgcggcgg
    11641 cctgaaaccg cacttcttcg gcacctatct aaatatcgcg gccaacgttc tcttttgggc
    11701 actgctctcc ggggttttca atcgcgaggg ctacgtcgtg tggcttctct gtcttcttac
    11761 cgcctgtggc gcatctctcg cctggggtct gaagcgccgg cagttttcct tcgtcgctta
    11821 tgccgccgtg tacggctacg tgggtgtcag cgcggttctc attcgcgaca tgcatgaaga
    11881 cactgctgtc ctaggctatt tcgtctttac tggtgtcgcc atgctcgtta tgctcgtcca
    11941 gatcgcgcgc cgcttcggga gggaagggtg agagcctaca gtgcatccag cgaagaagct
    12001 cggcgcgccc gcgatctgct gcaggactgg tggggcgaag gtcttctcac cgtagagcag
    12061 tacctgcgca tggaacaaga gaccgtttgt gagctccgcc gcaccaacat tttcctgcga
    12121 ctagtcctgt tgctttttac gctgattatc ctgggcgcag cggttgggct gtttttcgcg
    12181 ttgtttttct cgcatccggg ggagcaaacc cttggtattt ttctgctaat cttcgctgcc
    12241 ctctcttatg cggcagcgga aatcgccgtt accaaagccc agctctatcg cttcgggatc
    12301 gaggaagccc tcgccgcgtg ctcggtcggc tttctctgtg tggggatgca actcgcgctt
    12361 tttagcggtc tgttctcttc ccccacgcct gcccgaactg gatttctggt tcccgctgct
    12421 ggcgccgccg tttcgctctg gatgtggcat cgtttcggac tcccctatgc atctcttggc
    12481 gcgatgatct tcgtcgcctg gctgcccagc tactggacat cgtctccttc ggggcagcat
    12541 ctgatcgtcg cggctgtcta ctccgccggc gtggtaatgg tggccgcagt acgcccccgc
    12601 tatcgcttca cgtatctcga cagcgaatat tcgatcgccg aagcgctcct ctggctcggt
    12661 atctacctgg ctgtcaatct ccaactttcg tccgtggacc tgcgcgggta ttggtggagt
    12721 ggctctcggt ccaccgccca attctcaagg ctgttctact gggcgacttg ggtgctgatc
    12781 tggtgcttgc cccccgtcgt gctcgcacgc ggcctgcgcc ggaaggaccg attcgtcatc
    12841 gcagtcggcg cgctcgccgc catcctaacg ctggtcacca acaagcctta tcttggctgg
    12901 cagcggcaca cctgggatcc catgctgctg ggcgcattct tgatcggtgt cgtgctcctc
    12961 atccgccgtt ggctcgccaa ggcgccgggc ggaattcacc acggctttac tgcccagcgc
    13021 ctgtcgggaa aggacaaacg gtggatgaac gtcggggttg ccacaatcgg actcgcatcg
    13081 ccgaacatcg tgacgccaag agggcaaaca ggcggccccg acgtccactt cggtggggga
    13141 gattcaggag gtggaggtgc tacctccgat ttctgatgcg ccgggcagat cggttatccg
    13201 gttgttgtgg agttggactt cgcagaccgg tggttcccgg taacgggttt ggggaagtta
    13261 gcgcactcgg acgcgaaacg cccatcccgc ccttttggag gctgcactca gggcgcggcg
    13321 accagaaatc tgccggcggg ccgccgctag cacaccggac tgggcaaact ctgctactct
    13381 tagggcacca tgtcagccgc taatggagat aaagcgcgct tccaccgcgg acgaaagagg
    13441 actcttgcac ggagggagcg cagccgcgcg ctgcgcaaga aaccagccgc ccagccgccg
    13501 gagcgtccga gtggcgccac ttcatagtcc cgcggccgaa catctggcca gggtctaggc
    13561 ggctcggtcc ggtcgccttc atggcccgag gcccgccgcc gctctctcgc tccggagtct
    13621 gttagactcc tgctggtatg aaaagatccg tggctttgtt cttcttcctg ctgtacgtgt
    13681 gccccgcgca ggtcgtcttc cagcgcgcgg atcgtggcgg cgccatcatc gagatcgacg
    13741 cttcccggaa agccggttac acaatccccc gaacgatcta cggtactttc ctcgagccga
    13801 tcggcaagtc catctacggc ggtctctggg cccagatcct cgagaacccg agtttcgaag
    13861 agaacctgtg gaacgccggc aacattcgaa gaatgatcga tcgcgagccg gctctggagc
    13921 gcgcctcggc aatcggtctg cccctgccgt gggagccttt cgaccccgct cagggctcgc
    13981 gttatgagcc gcgctggggc gatgccgcca actcctcacg ctcgctgttc atcatggccc
    14041 tgccggacaa gcagaccggc gttcgacagg aggtctacct tccggtccat cggacgttgc
    14101 gctacaccgg tagtttccac gcccgtcact tcagcgggcc ggccgaagtc ggagtctcgc
    14161 tccgccggcg aaaccatccc gaaaccgtct tcgcgcgggc ggcgcttcag ctcgccggca
    14221 gcgcatgggc acgctatgaa ttcacgctgg aactgactcg cggacaagtg gccgcgctcg
    14281 agccggccga cttcgtcatc gcattgcggg atgaggggcg cgccctcgta gaccaggtct
    14341 tcctgtttcc cgccgatcac gtaaacggca tggaccccga tatgattgcg ctctcccgcg
    14401 cgctcaagac gccgctggtg cgctacggag gcaatttcac ctctggttac cactggcgcg
    14461 acggcgtcgg tcctattgat agacgtgtct ccatgctgaa ccagtcctgg ggaatgcccg
    14521 agtacaacca ctttggcacc gacgagttcc tcgagttctg ccggctgatc ggcgccgacc
    14581 cgcagatcgc gctgaacctc ggcagcggca cgcccgacga ggcggccgaa tgggtccatt
    14641 acgtcaatga tcgatggggc gaccgccgcg gcggcctgct ttgggagctc ggtaacgagc
    14701 tttgggggac ctttcagatc ggctatccga ctctcgagcg cgtcgccggg cgcacccgcg
    14761 ccttcgccca ggccgtccgc cgcgccgatc cgcgcgcccg tctgatcgcc accggcgccg
    14821 accccgacca ctttagcgaa tggaacgcag cgcagctcgg cctgcctccg gggtcattcg
    14881 attttctggc cacgcacttc gtcgtgggcg ccggcggagt tcgccgaagc aacccgtcct
    14941 cggagttcgt tacgctttcc gccttcgccc ttcccatcgg cctggagcgc aagctgcgcg
    15001 agatgaaaga gcagatcgac gccgtcccag agtggcgcgg gagaacgaag atcgccttca
    15061 ccgagtggct ctttcacggg ccggacgagc gggttccccg attcagcaat atgggaggag
    15121 ctctttgcgc cgcgggattc ctgaattcct tgatgcgggt cgccggcttc acgccggttt
    15181 ccgacatgac cggtctcatc gaattcggcg gcatctggaa gaagcgtggc cgggtctacg
    15241 gcgtcccggc ctactgggcg tttcggatgt actcgaacac ggacgcatcg gtccccgtct
    15301 ccacccgggt gacggtggaa cactacgaca tcgagcaggg caacaaccgg attcccgaca
    15361 tccgcgacgt gccttatctc gacgttgtgg cggcgcttaa ccaagccgga gaccgcctca
    15421 cactcttctg cgtgaaccga cacctgtacc gggacctgcc tgcggcgatc cggctttccg
    15481 gcttcgcccc gtccggccgc gccagtgtca agacgctcgc cggtacgagc atccaccagc
    15541 cgaatgacga gatgcgcccc gaagccgtcc accccgtcga cagcgtcgcc cccgccgccc
    15601 ccgagttcga atacaccttc ccgcgctcga gcgtgacggt gcttgaaatg accgccggcc
    15661 gatgaaacgg tctggaatct gcggtctaca cccggctggc agtcaggcaa gcttctttaa
    15721 ccttttatta tcttgaggct aacgccactg tctgctatac tcggactgga tgttcacccc
    15781 tccgcgcgcg cggacacggt tgaaacagac aggggacgtg ccccgagcgc gaagtcgtgc
    15841 ctgcctcacc cgttttccgc catagtcgcc tggaggtagc catactcgat tgccaatgaa
    15901 agtaagtgtc gagattagcc ggggcatcga atcacttttc ggtacccatg acgaaaatat
    15961 ccgcctgctc gaaaccggcc tcaacgtgag cacgcagttg ctggaagact gcctcgaaat
    16021 agaaggcgag gaggccaacg ttgctcgcgc cgagcgcatc ctcgaggact acgtgtccct
    16081 cgtcagggaa ggacatacct ttgccaacgg cgacctcaac agctacctgc gtgtggtcac
    16141 cgaggacccc ggcgtgcggc tccgcgacct tgtcctcagc ggccgccagc gcagttttgg
    16201 caagaaggt
//
LOCUS       DATIFQ010000455         2308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2258377, whole genome shotgun sequence.
ACCESSION   DATIFQ010000455 DATIFQ010000000
VERSION     DATIFQ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258377"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VLE22_22905"
     CDS             <1..1045
                     /locus_tag="VLE22_22905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17319.1"
                     /translation="GSGHNRSMRKTTLALTSVGLSIGLLPAADAPNAEITNGSLRVKI
                     YLPDAKNGYYRGTRFDWSGVVYSLQYKGHDYYGPWFQKTDPKVHDFIYEVADIIAGPC
                     SAIPGPVDEFRPLGWEEAKPGGSFIKIGIGALRRPDESKYDNYRLYEVADPGKWTIRK
                     RRDSIEFTQTLADSPSGYAYVYRKALRLVKGKPEMVLEHSLKNTGTKPIRTSVYNHNF
                     LVLDGKPPGPGVAITVPFQIQTRRPPNKELAEIQGNRIEYRKTLVGRDEVAFPVQGFG
                     DSATDNDIRIEDGRIGAGMRITADRPLSSVYLWSIRSVISVEPFISIAIEPGGEFTWK
                     SDYTYYTLPGNTK"
     gene            1042..2136
                     /locus_tag="VLE22_22910"
     CDS             1042..2136
                     /locus_tag="VLE22_22910"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HSB17320.1"
                     /translation="MRRREFGRRCAAGIAGVTVPWTTTALPAPRARKNALMHVGGDYH
                     SVAGPGITSKENLEYNLRHGVRHLTILARKRSQEGGWDLDELKRMKEDCDKYGVALEA
                     IRMDADYITLPKGPERDRELDAILGNIRKASQVGVKIITYHWTVIPIRRNRKTPGRGN
                     SAYAGFKLEGNWKDLPSGKSGKVSSEDYWERITYFLEKVIPTAREHDVKMACHPYDPP
                     GLPLGYQGADNWDSPSVFEAIKRYESIVDSPYNGFQLCLGTAAEGLKKPIVEILPIVQ
                     YLGERGKLHQIHMRNIRGGLHDFEEVYPDEGEMDFFKVMRILRDTQFAGSICPDHMPH
                     HPADAGSLQAFAFGYGYVKALIQAVNSEVG"
BASE COUNT          520 a          671 c          692 g          425 t
ORIGIN      
        1 cggatcgggg cataatcgat cgatgaggaa aacgactctc gctctgactt ccgtggggtt
       61 gtccatcggg ctgctgccgg cggccgacgc cccgaacgcc gagatcacca acggcagcct
      121 ccgcgtgaag atctacctgc ctgatgcgaa gaacggatac tatcgcggca cccggttcga
      181 ttggtcgggg gttgtctaca gcctccagta caaagggcac gactattacg gtccgtggtt
      241 ccagaagacc gatccgaaag ttcacgactt catttacgag gttgcggaca ttatcgccgg
      301 gccgtgtagc gcaatccccg gtccggtcga cgaattccgg ccactcggat gggaggaggc
      361 aaaacccggc ggaagtttca tcaaaatcgg cattggggcg ctccgaagac cggacgagag
      421 caagtacgac aactaccggt tgtacgaggt cgcagacccg ggaaagtgga cgatccggaa
      481 acgtcgcgat tcgattgagt tcacccagac gctggccgat tcgccttcgg gctacgccta
      541 cgtctaccgg aaagcccttc ggctggtgaa gggcaagccg gagatggtgc tggaacacag
      601 cctgaagaac accggcacaa agccgattcg gacctccgtt tacaaccaca actttctcgt
      661 cctcgacgga aagccgccgg gtccgggcgt tgcgatcacc gttccgttcc agattcagac
      721 gcggcgccct ccgaacaaag agctcgccga aatccaagga aaccggatcg agtaccggaa
      781 gacgctggtc ggccgtgacg aggtggcgtt tcccgtgcag ggcttcggcg acagcgccac
      841 cgacaacgac attcgcattg aggacggccg gatcggcgcc ggcatgagaa tcaccgcaga
      901 ccgccctctg tcgagcgtat atctgtggtc catccggtcg gtgatttccg tggagccctt
      961 tatctccatc gcgatcgagc ctggcgggga gttcacctgg aaaagcgact acacctacta
     1021 cactcttccg gggaatacga aatgagacga agagaattcg gcaggagatg tgccgccggc
     1081 atcgcgggcg ttacggttcc ctggaccaca accgcactgc cggcgcctcg ggcaaggaag
     1141 aacgccctga tgcatgtcgg cggcgattac cacagcgtgg ccggacccgg tatcacgtca
     1201 aaggaaaacc tggagtacaa ccttcgacac ggtgtcagac atctcacgat acttgccaga
     1261 aaacgctctc aagaaggtgg ctgggacctc gatgagctaa agcggatgaa ggaagattgc
     1321 gacaagtacg gcgtcgccct tgaagcgatc cgaatggacg cggattacat tacgcttccc
     1381 aaggggccgg aacgcgaccg ggagcttgac gccatcctcg ggaacattcg caaggcgtcg
     1441 caggtgggcg tgaagatcat tacgtatcac tggacggtca tcccgatccg gcgaaacagg
     1501 aaaacgccgg gacggggaaa ctccgcgtac gccggcttca agctcgaagg caattggaag
     1561 gaccttccct ccggtaagtc aggcaaggta agttccgaag actactggga gcgaatcacg
     1621 tactttctcg agaaagtaat cccaacggcc agagagcacg acgtgaagat ggcctgccat
     1681 ccgtatgacc cgcccggcct accgctcggc tatcaggggg ccgacaactg ggattcgccc
     1741 tcggtgtttg aggcgatcaa gcggtacgaa tcgatcgtcg acagcccgta caacgggttt
     1801 caattgtgtt tgggaacggc cgccgaggga ttgaagaagc cgattgtgga gatcctgccc
     1861 atcgtgcaat acctgggcga acgggggaaa cttcaccaga tccacatgcg caacatccgc
     1921 ggcggactcc acgacttcga ggaagtttat cccgatgaag gcgagatgga ctttttcaag
     1981 gtcatgcgca ttcttcggga tacgcagttc gccggatcga tctgtcctga tcacatgccg
     2041 caccatcccg ccgacgcggg cagcctgcaa gcgttcgcct tcggctacgg ttacgtcaaa
     2101 gcgttgatcc aggcggtaaa ctccgaagtc ggctaggccg tcacgcccgc ggaagaaatg
     2161 gggactgact tccatttcgc tgctgttgcg aaatggctgt ctgtccccat ttcttcgtga
     2221 gtcagtccct ggttttctgt ttccgcgcca ggtagaacgt gcggtagccc ggccggatgc
     2281 tcggatcatt cctcaacaac ggagcggc
//
LOCUS       DATIFQ010000456         5177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_150872, whole genome shotgun sequence.
ACCESSION   DATIFQ010000456 DATIFQ010000000
VERSION     DATIFQ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150872"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VLE22_22915"
     CDS             <1..355
                     /locus_tag="VLE22_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17321.1"
                     /translation="DVKTLREFDDRFTAPSFGFRDAAHYYGTQSANRHLDRIRIPSLL
                     IQAEDDPLIPFRVFDQPVVHRNPAIRLLATAHGGHMGFLSRHTPRFWADTIMMEWFSE
                     QQRNKQATARVNSLR"
     gene            352..1593
                     /locus_tag="VLE22_22920"
     CDS             352..1593
                     /locus_tag="VLE22_22920"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17322.1"
                     /translation="MKSGASFAESVWVATGSLRSSKLRSFLTLLGIILATTTLIAVMS
                     VIEGMDRYIANQVSDMGADGFRIRRMAMIGQWDPKKYLEMQRRNPQLTVDEFNFLKGK
                     ATLIREIGMEAFRGVSVRFGTESAEDVGMRGVTANMGVISNVQTAMGRFITDVEDRKR
                     VNVVFIGNDLREKFFTGVDPVGKTLSIDGRPFDVIGVAKPQGSVFGQSRDNFVMIPAE
                     TFFKMYGSRSGLGFNALAIDRAHFNQAMEEVRMLLRAYRGLKPNEDDTFGMFGSDTLR
                     EAWDQLTGVIAATAVAIVSVFMVVGGVVIMNIMLAVVTERTHEIGIRKSVGARQSDIM
                     NQFLVESSVLSAAGGVFGVLLAWAVAIAVREMTPVPMAIPASAVAVAVTLSAAVGLFF
                     GIYPARRAAKLDPIAALRAEQ"
     gene            1590..2840
                     /locus_tag="VLE22_22925"
     CDS             1590..2840
                     /locus_tag="VLE22_22925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17323.1"
                     /translation="MSGQEVLSAVGMALATIREHKLRSLLTVLGVIIGTGTVIAVGSI
                     ITGVDGAITNITRSFGPNTAFVFKFNIGFRGHITREEWMRKPLTYDHAMAIAERCPSV
                     QGVSPWLFSPNTFSGRGIDRARYKGNDMYQLDFAGTEEGYAEGGAERMKFGRFFTDTE
                     GRHRMPVIVIGEDVQKAWFPNVDPIGKWIEVNGHMFEIVGVMARPSFSFPGQEDRRFL
                     VPYFTMRKMFPQAKEHMIVVVAKDGKLAAALDEVRVALRQERRVPYNKPDDFWISTGE
                     QMLEEFHNITAIVALVMVVLSSIGLLVGGIGVMNIMLVSVTERTREIGIRKAVGAKRS
                     DIIAQFLTEAVVLTALGGLLGMAFGWLISLGSRIVFPTLPTSVPLWAAAMGVVVSVGV
                     GLFFGVWPATKAARLDPVVALRYE"
     gene            2923..3258
                     /locus_tag="VLE22_22930"
     CDS             2923..3258
                     /locus_tag="VLE22_22930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17324.1"
                     /translation="MGRWLCVVFLASATALASEQAAPKTESPDSSRKRALENALRGIE
                     KGKDLVWIKGQAFAVEPERCGHIVLMPADPDVDPKMVMPIPKEKEGNSSSSRMPKINT
                     MPPCQEEHR"
     gene            complement(3276..4169)
                     /locus_tag="VLE22_22935"
     CDS             complement(3276..4169)
                     /locus_tag="VLE22_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17325.1"
                     /translation="MGLRPQATGDAAAPAVSVLPHAPVELTRGRLRRLGEGLGKVVYA
                     SEHWVVKRERSPSEIIALIVLWKVLRRVERHLPGQLGRRLLEKPSKTIRFVRLLMQAV
                     VPAVPKSVWFTTHIGQVWRTYHFRNLRGEKLAEAHLAGTTLIPERVIFPPVRVTVGGW
                     PGWLIISEATERVEATLHQRLTELARAGRFDELEHWLNRFLDLRQAGWQRGVFSVDAH
                     LKNFGVSGDHVVLLDPGGLTNRWEDIAERLAFENSMERPHKRLGLGPLLGSRPDIAER
                     FDTRWKATVNLDAVRRQWPNH"
     gene            complement(4203..>5177)
                     /locus_tag="VLE22_22940"
     CDS             complement(4203..>5177)
                     /locus_tag="VLE22_22940"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HSB17326.1"
                     /translation="QTLRRVVQLAFFALNVWIGVEFFLFVRHYETGGASVRVPRPPGV
                     EGWLPIASLMNLKVWALTGQVPAMHPSGMFILIAFLAISFLLRKSFCSWLCPVGTISE
                     YLWKAGRQTFRRNFQLPRWADIALRSLKYILMGLFLYAVGSMSVDAIRGFLEGPYGVI
                     SDVKMLNFFRFLGVTGAVTIGVIAILSILIQNFWCRYLCPYGALMGLVSRFSPARIRR
                     DPVTCIDCAKCTKACPSLLPVDKLITIRSPECTGCLECVAVCPAAGALDLKVGKRRRI
                     PAWAMAAGIAVLFLGIVGYAQWAGHWQTNIPSRVYFDLIPRANDFGHP"
BASE COUNT          991 a         1543 c         1678 g          965 t
ORIGIN      
        1 tgacgtgaag acactccgcg agttcgacga ccggttcacc gcgccgtcgt tcgggttccg
       61 cgacgcggca cattactacg gcacccagtc ggcgaaccgg cacctcgacc gaatccgcat
      121 tccctcgctg ctgatccagg ctgaggacga cccgctcatc cctttccgcg tcttcgacca
      181 gccggtcgtc caccggaacc cggccatccg cctcctggcg accgcgcacg gcggccacat
      241 ggggttcctg tcgcggcaca cgccgcggtt ctgggctgac acaataatga tggaatggtt
      301 ttccgaacaa cagcggaaca aacaagccac cgctcgcgtc aactccttac gatgaagagc
      361 ggggcgtcgt ttgccgagtc ggtgtgggtt gcgacgggtt cgctgcgctc gagcaaactc
      421 cgtagttttc ttacgcttct gggtatcatt ctggctacga ccacccttat tgccgtcatg
      481 tcggtgatcg aaggaatgga ccggtacatc gcgaaccagg tctccgacat gggcgccgac
      541 ggtttccgca tccgccgcat ggcgatgatc ggccaatggg atccgaagaa gtatctggag
      601 atgcagcggc gcaacccgca attgacggtc gatgaattca attttctcaa gggcaaggca
      661 acgctgatcc gggaaatcgg catggaggcg tttcgcggag tctcggtccg gttcggcacc
      721 gagtcggcgg aagacgtcgg gatgcgcggc gtcaccgcca acatgggcgt gatctcgaac
      781 gtccagacgg cgatgggccg cttcattacg gacgtcgagg accggaagcg cgtgaacgtc
      841 gtcttcattg gcaacgatct gagagagaag ttctttaccg gtgtggatcc ggtcggcaag
      901 actctttcga tcgatggccg gccgttcgac gttatcggag tggcaaagcc gcaggggtcc
      961 gtgttcggac aatcgcgcga caacttcgta atgatcccgg cagagacctt tttcaagatg
     1021 tacggttccc gctcgggtct cgggttcaac gcgctcgcca ttgaccgggc tcacttcaat
     1081 caggcgatgg aggaagttcg aatgttgctg agggcgtacc gcgggctcaa gccgaacgaa
     1141 gacgacacct tcgggatgtt tggctccgat acgttgcgag aggcgtggga ccaattgaca
     1201 ggggtgatcg ccgccacggc cgtggcgatc gtctcggtct tcatggtggt cggtggcgtg
     1261 gtgatcatga acatcatgct ggcggtggtg acggaacgca cacacgagat cggcatccgc
     1321 aagtcggtcg gagcgcggca gagcgacatc atgaaccagt tcctggttga gtcgtcggta
     1381 ctctcggccg cgggcggcgt cttcggcgtc ctgctggcgt gggcggtggc gattgcggtg
     1441 cgtgagatga cgccggtccc gatggcgata ccggcgtccg ccgtggccgt cgccgtaacg
     1501 ctttctgcag ccgtgggact attcttcggc atctatccgg cgcggcgcgc ggcgaaactc
     1561 gatccgatcg cggcgctcag ggcggaacaa tgagcggcca ggaagtcctt tcggcggtcg
     1621 gtatggctct ggccacgatc cgcgagcaca agctgcggag cttgctgacg gtcctcggcg
     1681 tcatcatcgg aacggggacg gtgattgcag tgggttcgat catcaccggc gtggacggcg
     1741 ctattaccaa catcacacgc agtttcggtc cgaacaccgc attcgtcttc aagttcaata
     1801 tcggcttccg tggacacatc actcgggaag agtggatgcg aaagccgctc acgtacgacc
     1861 acgctatggc tatcgcggaa cgctgtccct ccgtgcaggg ggtgtcgccc tggctgttca
     1921 gtccgaatac gttttccggc agagggatcg accgggcccg gtacaaaggc aacgacatgt
     1981 accagctcga cttcgccggg acggaggagg gctacgccga gggcggcgcg gagcgaatga
     2041 agttcgggcg cttcttcacc gacacggagg gccggcaccg gatgccggtg atcgtgattg
     2101 gcgaggatgt gcagaaagca tggttcccga acgtagaccc gatcgggaag tggatcgagg
     2161 taaacgggca catgttcgag atcgtaggcg tcatggcgcg gccgtccttc tcctttccgg
     2221 gccaggagga caggcgtttc ctggtgcctt acttcaccat gcgcaagatg tttccgcaag
     2281 ccaaggaaca catgattgtg gttgtggcga aggatggcaa gctggccgcg gcgctagatg
     2341 aagtgcgtgt cgcactccgg caggagcgcc gtgtcccgta caacaagccc gatgatttct
     2401 ggatctcgac gggcgaacag atgctcgagg agttccacaa catcaccgcc atcgtggcgc
     2461 tggtgatggt cgtgttgagt tccatcgggc tgctggtggg cggcatcggg gtgatgaaca
     2521 tcatgctggt ctcagtcact gagcggacgc gagagatcgg aatccggaag gcggtcgggg
     2581 ccaagcgcag cgacatcatc gcccaattcc tgacggaggc ggtcgtactt accgcgctag
     2641 gcggcctcct tggcatggcg ttcggctggc tgatttcgct cggttcgaga attgtcttcc
     2701 caacgcttcc gacctccgtg ccgctgtggg cggcggcaat gggcgtggtg gtttctgttg
     2761 gcgtgggcct cttcttcggc gtctggccgg cgaccaaggc cgcccgtctc gacccggttg
     2821 tcgcattgcg gtacgagtga ccgggactgt tgttccgcgt tccggcgcta cactggggtt
     2881 ccccttgaca cggcccgccg gttgcggcgt aatcgaggtg ttatgggacg ctggttgtgt
     2941 gtagtgtttc tggcgtccgc caccgcgctc gcgtcagagc aggccgctcc gaagaccgaa
     3001 tcgccggatt cgtctcgtaa aagggcgctg gaaaacgccc tgcgcggcat cgagaaggga
     3061 aaggaccttg tctggatcaa aggccaggct ttcgcggtcg aacccgaacg ttgcggccac
     3121 attgtgttga tgcccgctga cccggacgtt gatccgaaaa tggtcatgcc aatcccgaaa
     3181 gagaaagagg gcaattcttc ttcaagccgg atgccaaaga tcaatacaat gcctccttgc
     3241 caggaggaac accgttagtc cggggcaggc tgcggtcaat gattcggcca ctggcggcgg
     3301 accgcgtcga ggttgaccgt cgccttccaa cgggtgtcga aacgctccgc gatatccgga
     3361 cgagaaccga gcaagggccc gaggcccagc cgcttgtgag gccgctccat ggagttctcg
     3421 aaagcgagcc gctcagcgat gtcctcccag cgattcgtca ggccgcccgg gtcgagcagc
     3481 acaacgtgat ctccgctgac accaaagttc ttcaggtgcg cgtcgacgga gaagacccct
     3541 cgttgccatc ctgcctgccg gagatcgagg aaccggttca accagtgctc cagctcgtcg
     3601 aaacgtccgg ctctggccag ttccgtgagc cgctgatgaa gcgtcgcctc cacgcgttcg
     3661 gttgcttcgc tgatgatcag ccagccaggc catcctccaa cggtgacccg gacgggcggg
     3721 aagatcacgc gctcgggaat cagggttgtg ccggcgaggt gtgcctccgc aagcttttct
     3781 ccgcgcaggt ttcggaagtg gtacgtccgc cacacttggc cgatgtgggt agtgaaccag
     3841 acgctcttcg gtactgccgg cacgacggcc tgcatcagca gccggacgaa ccggatggtt
     3901 ttcgagggtt tttcgagaag ccgccggccg agctgtccgg gaagatgtcg ctcaacgcgg
     3961 cgcaagacct tccagagcac aatgagcgca atgatttcag agggtgatcg ctcccgcttc
     4021 acgacccagt gctccgaagc gtagacgacc ttcccaagtc cctcgccgag gcggcgcagg
     4081 cgcccgcggg tcagttccac gggtgcgtgc gggagcacgc tcaccgccgg cgcggcagcg
     4141 tcgccggtcg cctggggcct cagacccatc acctcaactc tagctcgata ccgttcggca
     4201 ggctacgggt gcccgaagtc gttcgcccgg gggatgagat cgaagtacac gcggctcggg
     4261 atattggtct gccagtggcc ggcccactga gcgtacccca cgatgccgag gaacaacacc
     4321 gcaataccgg ccgccatggc ccatgctggg atgcgacggc gctttccgac tttgaggtca
     4381 agtgcgcctg cggcggggca gacggcgacg cattccaggc aaccggtgca ttccggagag
     4441 cggatcgtga tcagcttgtc caccggcagc agcgacgggc aggccttggt gcacttggcg
     4501 caatcgatgc aggtcaccgg gtctcggcgg atgcgcgccg ggctgaaccg tgacaccagg
     4561 cccatcagcg ccccgtacgg gcagaggtag cggcaccaga agttctgaat taagatcgac
     4621 aggatagcaa tcacaccgat cgtcaccgct cccgtgacgc cgaggaaacg gaagaagttc
     4681 agcatcttga cgtcggagat caccccgtac gggccttcaa ggaagccgcg gatcgcgtcc
     4741 acgctcatgc ttcccacggc gtacaggaac aaccccatca ggatgtattt taggcttcgg
     4801 agcgcgatgt cggcccatcg cggcagttga aaattgcggc ggaaggtctg acggccggcc
     4861 ttccagaggt actcggagat cgtgccgacc gggcacagcc agctacagaa cgatttgcga
     4921 aggagaaacg aaatcgccag gaaggcgatc aggatgaaca ttccagatgg gtgcatcgcc
     4981 ggaacctggc ccgtcagcgc ccacaccttc aggttcatca gcgaggcgat cgggagccag
     5041 ccttctacac cgggagggcg gggaacccga accgaggcgc ccccggtctc gtaatggcga
     5101 acaaagagga agaactccac cccgatccag acgttcagag caaagaatgc aagctgcacg
     5161 acgcgccgca gtgtctg
//
LOCUS       DATIFQ010000457         3929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1303091, whole genome shotgun sequence.
ACCESSION   DATIFQ010000457 DATIFQ010000000
VERSION     DATIFQ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303091"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VLE22_22945"
     CDS             complement(<1..279)
                     /locus_tag="VLE22_22945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17327.1"
                     /translation="MVRFLRRIAPFTSIALLLALAYVGWEFYSRSRAQRRTQAQIEEK
                     QRAAYPGFSRESGSGVKILQFYASPAVLVEGQTAILCYGVSNTASVRIE"
     gene            418..1482
                     /locus_tag="VLE22_22950"
     CDS             418..1482
                     /locus_tag="VLE22_22950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17328.1"
                     /translation="MRAIVSLLAAAMFTVPLAAQKTIEIRGAYSDPKPFWNKGARLDE
                     YGVNAVFVYGGGITPELLARAKAEGARVYSEFATLNGHGYVEKHPEAWPINEKGEREG
                     PATWFQGVCPTEPGFREHRLKQLADLLDKHDIAGVWMDYFHWHAQFEDPSPILPETCF
                     SPTCLAAFAKSTGVRIPDGTTAEKARWVLTKHEKKWRDWRCSVLVDWAREFRRVIREK
                     RPGALLGLYHCPWTDDEFSGARRRVLGLDFDMLKNEVDAFSPMVYHGRMGRPASWVGE
                     YVEWFSKRLGLRRGQSPKVWPIVQAHNEPSVIPAEEFERVMRFGLSGASTGVMMFTFS
                     SVASDPAKMEVLKKLYTGGR"
     gene            complement(1546..3237)
                     /gene="ggt"
                     /locus_tag="VLE22_22955"
     CDS             complement(1546..3237)
                     /gene="ggt"
                     /locus_tag="VLE22_22955"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSB17329.1"
                     /translation="MKREREIAALLAALVMISGEARSASRTHARSMVITRQGIVATSQ
                     TLASQAGAQILARGGSAVDAAIAANAVLSVVEPMSCGIGGDLFAIYRDAKTGKLSGIN
                     ASGWAPKNLSIDYLEKKGLTTMPQLGILSVTVPGCVDGWEKLHKRFGKLPWADLFRPA
                     IYYATNGFPVTEMIQQNWYGEMAKLTQERYARPLFLPGGAAPRIGQVFRNPDLANALE
                     LIAKNGAAAFYKGPITQAILRVSEANGGVLEAADFSEFESEWIDPISTDYRGWKVYEL
                     PPNGQGIAALEMLNIFETFPMSEHEPLSADAFHLKIEAQKLAYQDLQRYVGDPRFAKI
                     PVSGMLSKPYAAERARLINPAKANCQAAPGEPPFGPGDTIYLSVVDRDGNIVSLIQSI
                     YLAFGSGIPVNGMGFHLHNRGGLFVLDRQHPNALAPRKRPFHTIIPAYMEKGDMHVGF
                     GIMGGLNQAQAHAQFVSNIADHDMNIQAALEAPRFTKRDFGGCEVVVEGRLPAETRSV
                     LLDRGHKLDVRAAFDDIVGGGQVVLHNSTSKVNYGASSPRKDGAAIPEPDPYFAK"
     gene            3458..>3929
                     /locus_tag="VLE22_22960"
     CDS             3458..>3929
                     /locus_tag="VLE22_22960"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB17330.1"
                     /translation="MNRRNFLSASGAMVGAVALNAFPYRLFASPAKKLASDQVKLGPM
                     GVTVSRLAMGTGTNGVGGSSNQTRKLGLRGVADLFRAAYDHGVYFWDSADQYGTHPHL
                     KTALQSIAREKVVILTKTHASTEAEMRADLDRFRRELGTDYIDIVLLHCMMDADW"
BASE COUNT          728 a         1199 c         1281 g          721 t
ORIGIN      
        1 ttcgatccgc acggaagccg tattgctgac gccgtaacag aggatggccg tctgtccttc
       61 cacaagcacg gcgggagagg cgtagaactg gaggatcttc acgccgctcc cactctcccg
      121 gctgaagccg ggatatgcgg cccgctgctt ctcctcaatc tgcgcttgcg tacgacgctg
      181 tgcgcggctg cgggagtaaa actcccagcc cacgtaagcg agggctagaa gtagcgcaat
      241 cgaggtgaac ggcgcaatgc ggcgaagaaa ccggaccatg acccaagata cgaagcaagc
      301 gaccggccag ttcggtattc ggctaagtca cggcgggtca aacgtcgagc gcggtgatcc
      361 aggagcgccg tgtccggtat tgggacggcg ccgttcgctt caggtacagt tccaagtatg
      421 agagccatcg tcagcctcct cgccgccgcc atgttcaccg tgccgctcgc agcccagaag
      481 accatcgaga ttcgcggcgc ctactccgat ccgaagccgt tctggaacaa gggggcgcgg
      541 ctcgatgagt acggcgtgaa cgcggtcttc gtgtacggcg gcggaatcac accggagctc
      601 ctcgcgcggg cgaaggcgga gggagcccgc gtctactcgg aattcgcgac cctgaacggc
      661 catggctacg tcgagaagca ccccgaggcg tggcccatca acgagaaggg tgagcgcgag
      721 gggccggcaa cgtggtttca gggagtctgc ccgaccgaac cgggcttccg cgagcaccgg
      781 ctgaagcaac tggcggacct gctcgataag cacgacatcg ccggcgtctg gatggactac
      841 ttccactggc acgcccagtt cgaggacccg agtccgattc ttccggagac ctgtttctcg
      901 ccgacatgcc tagccgcatt cgcgaagtca accggcgtga ggatccccga cggcacgacc
      961 gcggagaaag cccgctgggt tctgacgaag cacgagaaga agtggcgcga ctggcgctgt
     1021 tcggtactgg tggattgggc gcgcgagttc cggagggtga tccgtgagaa acgacccggc
     1081 gcgcttctcg ggctctacca ctgcccgtgg acggacgacg agttttcggg cgcccgccgc
     1141 cgcgttctcg gcctcgattt cgacatgctg aagaacgaag tcgatgcttt ctcgccgatg
     1201 gtctaccacg gccgtatggg ccgtccggcg tcgtgggtgg gcgagtatgt cgagtggttc
     1261 tcgaagcgac tggggctccg gcgcgggcag tcgcccaaag tctggccgat cgtgcaggcg
     1321 cacaacgaac ccagcgtgat cccggcggaa gagttcgagc gagtgatgcg gttcggcctt
     1381 tccggtgcgt ccaccggcgt gatgatgttc accttcagtt cagtggccag cgacccggcg
     1441 aagatggagg tgctgaagaa actgtacacg ggcggccggt aggcagggcg ctgcaaaacc
     1501 cgccggagat atcgaccatc acaggctgaa gcctgtgcca ccggcttact tggcgaagta
     1561 cgggtccggc tcgggaatcg ccgcgccgtc cttgcggggc gaggaagcgc cgtagttcac
     1621 cttcgatgtc gagttgtgca ggactacctg accgccgccg acgatgtcat cgaacgcggc
     1681 gcggacgtcc agtttgtggc cgcggtccaa gagcaccgac cgggtctcag ccgggaggcg
     1741 gccttcgacc accacctcgc atccgccgaa gtcgcgcttg gtgaaccgcg gggcttcgag
     1801 cgctgcctgg atgttcatat catggtcggc gatgttcgac acgaactggg cgtgcgcctg
     1861 cgcctggttc agtccgccca tgatgccgaa cccgacgtgc atgtcgccct tttccatgta
     1921 ggccgggatg atggtgtgga acggccgctt gcgcggagcg agcgcgttgg gatgctgccg
     1981 gtcgagaacg aacaggccgc cgcggttatg gagatggaat cccattccgt tcaccgggat
     2041 gcccgagccg aacgccagat agatgctctg gatcagcgag acgatgttgc catccctgtc
     2101 gactactgac aagtagattg tgtcgcccgg gccgaacggc ggctcgccgg gggccgcctg
     2161 gcagttagcc ttcgccggat tgatcagacg ggcacgttcc gcggcatacg gtttcgagag
     2221 cattcccgac acggggatct tcgcgaagcg ggggtcgccg acgtagcgct gcaagtcctg
     2281 gtaggcgagt ttctgggcct cgatcttgag gtgaaacgcg tccgcgctca gcggctcatg
     2341 ttccgacatg ggaaaggtct cgaaaatgtt cagcatctcg agcgcggcga tgccctggcc
     2401 gttgggcggc aactcgtaga ccttccagcc gcgataatcg gtcgagatcg gatcgatcca
     2461 ctcggattcg aactcggaaa aatccgcagc ctcgagcaca ccgccgttcg cctccgaaac
     2521 gcgcaggatg gcctgagtga tcgggccttt gtagaaagcc gccgctccgt tcttggcgat
     2581 caactccagt gcattggcga gatcgggatt ccggaaaacc tggccgatcc tcggcgcggc
     2641 gcctccggga aggaaaaggg ggcgggcgta gcgctcctga gtgagcttgg ccatctcgcc
     2701 gtaccagttc tgctggatca tttcggtgac cgggaatccg ttcgtggcgt agtaaatggc
     2761 cgggcgaaac aggtcggccc atggcagctt gccgaaacgc ttgtggagtt tctcccagcc
     2821 gtccacgcaa ccgggcaccg tcaccgatag gatgccaagc tgcggcattg tagtgaggcc
     2881 cttcttttcg aggtagtcga tcgacaggtt ttttggcgcc cacccgctgg cgttgatccc
     2941 ggaaagtttg ccggtctttg cgtcgcggta gatagcgaac agatcgccgc cgatgccgca
     3001 cgacatcggt tcgaccaccg agagcacggc gtttgcggcg atggctgcgt cgaccgcgga
     3061 gccaccccgc gccagaatct gcgcaccggc ctgcgacgcc agcgtctggc tggtcgccac
     3121 gattccctgc cgcgtgatca ccatggatcg cgcgtgcgtt ctgcttgcgg agcgtgcctc
     3181 tccagatatc atgaccagtg cggccaacag ggccgcaatc tccctttcac gtttcatgga
     3241 gtactcattc taccctctac tcccgggaag ctggaaagaa aacctgggga cacgtctgcg
     3301 taccgttgca agtggctcat ggcacgaggc caccccgaag tgtccccggt tttcttcaca
     3361 gcttcccgga gacgaaattc cgagttccgc gttcggaact cccgctcgac aggctgtccg
     3421 gagggctgtg ctaggctgta gggaactgga caagcctatg aaccgacgga actttttgtc
     3481 tgcctccggg gcgatggtag gtgcagtcgc tctgaacgcc tttccctacc gtcttttcgc
     3541 gagtccggca aagaagctcg cgagcgatca ggttaagctg ggtccgatgg gagtcacggt
     3601 gagccgtctg gcgatgggga ccggcaccaa cggcgtcggc ggcagctcca accagacccg
     3661 gaagttgggg cttcgggggg tcgccgatct tttccgcgcg gcgtatgacc acggcgtcta
     3721 tttctgggac tcggcagatc agtacgggac acatccgcac ctgaagacgg cgctccagag
     3781 catcgcgcga gagaaggtgg tcattctaac gaagacgcac gcctccacgg aagccgagat
     3841 gcgggccgac ctcgaccgct tccggcgcga actcggaacg gactacatcg atatcgtact
     3901 cctgcactgc atgatggacg ccgactggc
//
LOCUS       DATIFQ010000458        19074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_347060, whole genome shotgun sequence.
ACCESSION   DATIFQ010000458 DATIFQ010000000
VERSION     DATIFQ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19074
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347060"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..152)
                     /locus_tag="VLE22_22965"
     tRNA            complement(58..152)
                     /locus_tag="VLE22_22965"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Sec,seq:tca)
     gene            190..263
                     /locus_tag="VLE22_22970"
     tRNA            190..263
                     /locus_tag="VLE22_22970"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:222..224,aa:Gln,seq:ttg)
     gene            525..1103
                     /locus_tag="VLE22_22975"
     CDS             525..1103
                     /locus_tag="VLE22_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17331.1"
                     /translation="MHLAQHSLLLAAPRLSLLVEELLGNTTVEFIHVHGFDPCLHLVV
                     PGLQPFDGLFAGGSLRPIRLEHNIAKPIEYRLRDNELLQNIGELAGEDFLARVRLWAF
                     ASVAGTVVVHVSALLDFAHKRASAVATMHQTRVGEFMLYLPRFVLGTRIQQLLDPLPT
                     LAGHERLVRALVHGAVPFELPHVQAVAQNPVD"
     gene            1395..1796
                     /locus_tag="VLE22_22980"
     CDS             1395..1796
                     /locus_tag="VLE22_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17332.1"
                     /translation="MYQLVRGTRIGTIDAAFFDQMNGDTKIVQGDPVLQIAVEAVSLL
                     HQDRPAHAPMPLQIEQHRRELRTAAHPGRFDINEFLRDDKPAAGGILPQEAKLRWDAE
                     AFLLVLVGNPRVQYHLGSWLHRAASGRSRHG"
     gene            complement(2364..3563)
                     /locus_tag="VLE22_22985"
     CDS             complement(2364..3563)
                     /locus_tag="VLE22_22985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17333.1"
                     /translation="MATEGKLIPIPLSDEDKERLSVALGGPGAFSMFEESEFIAQSGC
                     PPEELERWLESCWLQHDIRAVLGGLEKVGPQPLLLVGFEDQYATVRLFCPTEKLLWEN
                     AIEDGLTRIRELIATRPWPQSAEEITSQIAALTAACTARELPTSEIKLLWFALTVAIQ
                     VLVELRGRPYTDQMKQLHAAMVAFAQACHERGSDLSPQAKYSEARVSSRRALSENHNP
                     VLDGFPELVAIAREICSQELAAYMAEDVRLSHDQFLDMLHVITHDRADPATVANPSAR
                     IRAALSREHRRSQMLYRKRWMPLSPAQTTGLEDPHAVESAAIAKIDWERGKRDLELPP
                     DQRRAVEARFDGLNLQASGVPDELACDPTHLESVRRSLEPDRRWGQRLRKRFAAYAPK
                     RNPARQS"
     gene            4426..4566
                     /locus_tag="VLE22_22990"
     CDS             4426..4566
                     /locus_tag="VLE22_22990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17334.1"
                     /translation="MTRTPVVLARRLAEAWCRFAHQGIYWPVCGQYRCKVCLLTHAVH
                     WP"
     gene            4719..5294
                     /locus_tag="VLE22_22995"
     CDS             4719..5294
                     /locus_tag="VLE22_22995"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HSB17335.1"
                     /translation="MRAAIYARVSTVDQHCEMQLRELREYCQRRGWTIAGEYVDAGWS
                     GAKASRPELDRIMRDAAAHRCDAVLVWKLDRFGRSVLNLSEQLQRLSSHAIRFLAISQ
                     AIDTDQSNPTSRLLLHMLAAVAEFERELIKERVKAGMAAAKHRGTRIGRPTVALDQRR
                     ARKLHKSGFSQRAIAEALGVGKGTIARLLAA"
     gene            5847..6092
                     /locus_tag="VLE22_23000"
     CDS             5847..6092
                     /locus_tag="VLE22_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17336.1"
                     /translation="MSEAHLKAEIERLRAENEALRKSPSRGQVTLKVSEKGAVSVYGL
                     GRFPVTLYKEQWEKLLAMADSIRTFIAENDHLLKKKE"
     gene            6109..6789
                     /locus_tag="VLE22_23005"
     CDS             6109..6789
                     /locus_tag="VLE22_23005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17337.1"
                     /translation="MEPTPLRTIEAVLDEFLTEQRSCLSPKRATDYEDIIELLKRFLN
                     GYWPGHDQAEYDRTTAAGGSFCTSFGPEEMLEAYGEFLGYYMTHKVIAGKETLRLAGT
                     VTKKLAAWLAEKEYVEDVEEAHERAKEAVRELPVATDALGILRNHVEKQPPRKCSEEI
                     QDHFTVAHVQPGRLWLTPLTERPDIIGPIPIPEEASRLLQEHWDISGSVGRTRQGWVL
                     LEVWNISP"
     gene            6947..7300
                     /locus_tag="VLE22_23010"
     CDS             6947..7300
                     /locus_tag="VLE22_23010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSB17338.1"
                     /translation="MGGNLVFLDLGQQRWAVYAHLQPGSLRVKQGDSVRRGDVIARLG
                     NSASYSAHLHFHIVDGPDPFSSEGVPFVFDEFTHEGTVHRDEMPAGGWTIRFVERQVE
                     PNSEFRRLPASNAVG"
     gene            7769..8233
                     /locus_tag="VLE22_23015"
     CDS             7769..8233
                     /locus_tag="VLE22_23015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17339.1"
                     /translation="MRHILSVLFAAALLPVTAAEPPGFVFWKSTDLKSYTEKLAPKVN
                     EKKFANEQIASWGNHSAMMVYRKGDGEAEVHEKTVDVFVVQEGRGTLVIGGKVVGGKQ
                     TGPGEIRGASIQGGVNKPLAPGDVVHIPANIAHQVLVAPGAEITYLILKVNM"
     gene            complement(8294..8974)
                     /locus_tag="VLE22_23020"
     CDS             complement(8294..8974)
                     /locus_tag="VLE22_23020"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HSB17340.1"
                     /translation="MTNDRFLATALWLLVAMVSIACGEEPKRSARTGNQVALPAPACA
                     GGMPLQEALARRVSVREFANRALDQQELSQLLWAAQGITHGGSRRTAPSAGALYPLEL
                     YVATRDGFFHYEPRGHKLQRLAEADLRTIISKAALGQKPIADAAAVFLFTAVSARTAQ
                     RYGEARAPRYVHIEIGHAAQNLLLQAVALNLGGVPVGAFDDPQLQKAIPVPRDHAVLY
                     LVPVGHLR"
     gene            complement(9069..9263)
                     /locus_tag="VLE22_23025"
     CDS             complement(9069..9263)
                     /locus_tag="VLE22_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17341.1"
                     /translation="MITYRTGNNLDMDQVIELYRASTLGERRPIDERERMGEMLRNAN
                     LGKRPGRPSAQCWTGRRRRS"
     gene            complement(9709..10977)
                     /locus_tag="VLE22_23030"
     CDS             complement(9709..10977)
                     /locus_tag="VLE22_23030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB17342.1"
                     /translation="MTASGEGGRSRKLTWLVFLVFFLISILTNIMGPLIPEIIRGFRV
                     SLAAAGLLPFVFFLAYGLVSIPAGIVMERTSVKTVVIGALVLEVAGALLLPLRPTYAT
                     ALASFLVVGTGAAALQVVINPLLRIAGGEEHYAFNSALAQFTFGTGSFLGPLLYSYLV
                     LRVGQESGGSFWLDPLRALTPAGLPWIAIFWVMAPAGLVVLAVTAAVRLPRRIHGEEG
                     AGAWANYRRLLGLPLVWLYFATMFLYVGLEQGLANWISQFLFTYHRFDPRTTGALAVS
                     WFWGSLTLGCLAGGALLKLFDSRAVLIGTTLGATAAFTAAIAGPASVSLVAFPVSGLF
                     LSVMWPIMISLGLNSLDHSHGVFAGILCTAIVGGAAFPPVIGHLGDLLGLRAGMLVLY
                     LSLGWVLSVGFWAKPLIRNATLRGREEADA"
     gene            complement(10974..12236)
                     /locus_tag="VLE22_23035"
     CDS             complement(10974..12236)
                     /locus_tag="VLE22_23035"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HSB17343.1"
                     /translation="MTRRDWMAEFRGNLLDGILRFWLDYGIDRQYGGAMGWLGRQGRP
                     AGPGTKSVAQQGRLLWALCQIYRRYPTPACADAAAWVLDFLRRKMRDPEHSGYYWLVD
                     REGNLLDGMKLLNPMAYVLEALAAYGQAFCDDGALEEALDLFRLMDRHAHDGAHSGYR
                     VAFTQNWQPILEYRSGPRVSVTACVAPVDDVHPVLADSHGRKSSDWHLGLLEAFATLY
                     DVTGDRAAAERLEELLEIFAARVVDPRQGYCRLYFNEKWEPEDWNGDALRCLYGLDME
                     ASWLMTDAAQLLGRPQDPLVRRAALSLVDHALRDGFDHENGGLYLEGPAAGPADCRRK
                     EWWQQAEALVGTLNAWQLAGDARYREAFERQSAYVLDVFTDHEYGDWFAAIEPDGTID
                     ATKAGPWRGPYHATRACLEVMRRLGGTL"
     gene            12738..13718
                     /locus_tag="VLE22_23040"
     CDS             12738..13718
                     /locus_tag="VLE22_23040"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HSB17344.1"
                     /translation="MTLSRSVKMFPLYTVVVVGATCLVGAAVAQGPAEDNLPFTLNGK
                     TWVSKKAFIDSGGRCATRNVDSIEADQIEAALARFLAAAGQGSSTKPGKPGGGPPPPV
                     TSVVVSVYFHVINNGPGIENGDVPQSQVEGQIAVLNAAYAAEGFSFSLVSTDRTTNSA
                     WYNMTPGSQAERQAKAALRVGGADALNIYTANLGSNLLGWSTFPWGYAGDPTDDGVVI
                     LYSSLPGGTAVPYNEGDTATHEIGHWAGLYHTFQGGCNTKKGGDLIADTPAERSPAYG
                     CPTGRDSCKADPGLDPIYNFMDYTDDACMFEFTTDQGTRMGAMWTQYRQP"
     gene            complement(13715..13993)
                     /locus_tag="VLE22_23045"
     CDS             complement(13715..13993)
                     /locus_tag="VLE22_23045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17345.1"
                     /translation="MTSYSRSEEQLQIRIRTDWDTDEEFTLRFAEGENPAGRFLLRSN
                     TFQPGDWKAIESTKGRVRPGVRATVWECKADGRPQLTIDWLPPLESQN"
     gene            14831..15331
                     /locus_tag="VLE22_23050"
     CDS             14831..15331
                     /locus_tag="VLE22_23050"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HSB17346.1"
                     /translation="MRNAVKRNFILQQGFENPAPALCPLQYSALLFQPRIVFIWVVAG
                     VLFQSPAVFAALCAVLWWSALFPKLNPFDALYNRTIGSRPGAFRVGPAPAPRRFAQTM
                     AGAFALTSALLIHAGFRLAAYVVEAIFSAAVLALAIGGFCLGSFVYHLLRGHGRFARQ
                     TLPWAS"
     gene            15884..18268
                     /locus_tag="VLE22_23055"
     CDS             15884..18268
                     /locus_tag="VLE22_23055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HSB17347.1"
                     /translation="MPSLLEDIRYSVRTLWKKPATAAVVFFTLLVGIVLNTSVFSICN
                     VLWFRPLPFKNPDQLVVLLQGNPRQGSERLCSQRDFDFLRQHARTVEALSAAGPRGFN
                     LSSGWPGGPEPRDASGAAVTDGFIETLGFRPIAGRLFTTADFQAGAPQVVLISERLWR
                     TWLAGADVVGSQVSVDREKATIVGILPRNFRFLTGGYDVVTPLTQGRTQREVEANNLI
                     VLARMRPQQAPPAMQAEMTALMRERLTGEAGWGLRVFRLRDQYMREARILYPVLLAAA
                     WLILLIVCANISNLLLARSAARRKEMALRLSLGAPRSEPARLVLVEGVLLAVLAGAAA
                     LPVCLWIRNALVAAVPDMAEIVVDWRVMGFTLLAALLAGLVFAIGPALSVLRIDVNAA
                     LKEEGAGVSRRSAFRLRRALVAGEMAVAMVMLTGTGLLLRAVAGMLTVDAGFRSAGTL
                     VARANLPPAVYPDTAHRAAALTRLSDAVRAMPGVDSVAVVSSTPMQGEIRRLRLEVAD
                     RPAPPEGETIRAALTSAGPGYFRTAGVPLVRGREFTEQDDAGGPGVAILNRTLVKALW
                     GEGANVLGQRLRTDPKDDWLAVVGVVGDTRQDLMREPFPEVYVPYRRICPPSATLVVG
                     TRLPPEALAGPVRAALRALDPELPFSGLRSLEDLRRDYIPPVYTIMLAAFSLLALALV
                     FVGLYGLVSFYVVESTREIGVRISLGALPRNVLALVVRQGVRLAVIGVLIGLAGSIAV
                     GKLLSGLLPTVIRPFDAGLFLAVSATLLSVAVAASLIPARRAIRLDPAANLRHF"
     gene            18709..19062
                     /locus_tag="VLE22_23060"
     CDS             18709..19062
                     /locus_tag="VLE22_23060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17348.1"
                     /translation="MPRGKRLDPRILEAALVGLEAQRQRTDEQIVQVRALLGGDRSSS
                     RAERTTRPRRRISAAGRRRIAAAQRQRWAQVRQAKAEGTKPKRRLSAAGRKRIAEAAR
                     KRWAQARAKKASSAA"
BASE COUNT         3588 a         5933 c         5928 g         3625 t
ORIGIN      
        1 aaagggcgtt tagtttggaa acaaacaaat ggaggggctg tgtcaggaaa gaggcagtgg
       61 cggaagccag tgggagtcga acccacctac gacacgaggt gccgcacaac gggtttgaag
      121 cccgcacccg gcgccggcca agaatggctt ccgggggaat tgtagcgaat ggtaagatgg
      181 agggcaaatt ccccggtcgt ctaatggtag gacagcggcc tttggagccg tgaatcgtgg
      241 ttcgaatcca tgccggggag ccagttctcc aaatcccgtc ctgacccgac gatctccttt
      301 tcttttctag agctttcctg gttcgaatgc ctcaatattt cgaacggttc gaacaaggtc
      361 gtccgcaaca ctccgtgttg ccagctggcg cgtttgatca gcacctgcag cagacgccgc
      421 tgctctgtgg cgggttgctg ctggaacaac tggcacgcct ggctcgttag ccgcaacgca
      481 tcgatagcct catcgaccgg cgccggcgcg gcattctgga cctcttgcat cttgcgcaac
      541 acagcctgct gctcgccgcg ccacgcctca gcctgcttgt cgaagaactc ttgggtaata
      601 cgaccgtcga gtttatccat gtacatggtt tcgatccgtg cctgcacttg gtcgtaccgg
      661 gcctgcaacc gtttgacggc ttgttcgcgg gtggctcgct ccgtccgatc agactcgagc
      721 acaacatcgc taagccaatc gagtatcggc tgcgggataa cgagctgttg cagaatattg
      781 gcgaactggc cggtgaggat ttcctggcgc gtgtacggct ctgggcattt gcctcggttg
      841 ccggtacagt ggtagtacac gtatcggccc ttcttgattt cgcccacaaa cgtgcatccg
      901 cagtggccac aatgcaccag acccgtgtag gcgaattcat gctttacctt ccgcgttttg
      961 ttctcggcac gcgcatccag cagctcctgg acccgctccc aacactcgcg ggtcacgagc
     1021 ggctcgtgcg tgccctggta cacggcgccg tcccattcga actcccccat gtacaagcgg
     1081 ttgcgcagaa tcccgtggac taaactcttg gtgattctac ggccgcgcag agttatgccc
     1141 tcgccgcgaa gctcggcgac gagggacttg agagagtacc tggccgagac gaaccggcga
     1201 aacatttcgc taacgacggg cgcgttctgt gggtcgggga taatgatccg cttgccgtcg
     1261 ggaccgtcag cattgaggta tcccgctcga gcgtagctgg ggtagacacc ggagcgcgcc
     1321 ttctccaaca tacccttccg ggtctcctca ccgaggttga gaacatagtt gcgcgctatt
     1381 aagacgttga acccgtgtat caactggtcc gcggaacccg aatcgggacc attgatgcgg
     1441 ccttctttga ccaaatgaat ggtgatacca agatcgtcca gggtgatcca gtccttcaaa
     1501 ttgcggtaga ggcggtcagt cttctccacc aggatcgtcc ggcacacgcc ccgatgccgc
     1561 tgcagataga gcagcatcgc cgtgaactgc gcacggccgc tcaccctggc cgtttcgaca
     1621 tcaacgaatt cctgcgcgat gacaaacccg cggctggtgg catactcccg caggaggcga
     1681 agttgcgctg ggatgctgaa gcctttctcc tggtccttgt cggaaacccg cgcgtacaat
     1741 accacttggg cagttggctg caccgtgccg cctcgggacg atcgcgacat ggttaaggtt
     1801 ctcgctggca tgacacctcc aacagtgcca aattcctggc tggtaaacat tggtcttcag
     1861 ggtcggacgt acggggatgc cgggccgtgg gccggtgtgc ggtgcaacct gacacccacg
     1921 cacaaagaac agcctcggtg gaccgctgcg cggcgtcgca caagatgcat ggtctgatcg
     1981 tcatgggcgt ggcttgcgga tacaaacaac attaccaact tcgaattcgt tcgtcttggc
     2041 ctgccggtcc caggtacgta agatgctgat gaactccagc agtctggctc gcgcccgcgc
     2101 ccattcggca tcggttaagg cacgaccacc atggcgttcg agcgctgccc gtgctgcaac
     2161 ctctgagcgt gctggatacg tctcggaacg tccgtcaagg aggttgaatt tctgcgttgc
     2221 catgtcgaag gtcagaccgt tgatccgtcc accgccagca agcctggcac gtagggtcta
     2281 ctggtcccct catctacaca ggcatcgaga atcagttttc aggacagatt tcatggagat
     2341 ttatttcctg gatttccagc aacttaggat tgtctggcgg gatttctctt gggagcataa
     2401 gccgcaaagc gcttgcgtag cctctgtccc catcggcggt ccggctccag ggatcgccgc
     2461 acgctttcca ggtgtgtcgg gtcacaggcg agctcatctg gcacgccgga tgcctggagg
     2521 ttgagaccgt caaatcgggc ctctacggct cggcgctgat ccggcggcag ctccaagtcg
     2581 cgcttgccac gttcccaatc aatcttggca atcgccgcag attcgacggc gtgtgggtcc
     2641 tcaaggccgg tggtctgtgc gggcgatagc ggcatccagc gcttacggta cagcatctgg
     2701 ctgcgtcgat gctcgcggga gagcgccgcg cgaatcctgg cggatggatt ggccactgtt
     2761 gccgggtcag cccgatcgtg cgtaataacg tggagcatgt ccaggaactg atcatgcgag
     2821 aggcggacgt cctccgccat gtaggcggcc agctcctggc tacatatttc ccgcgcaatc
     2881 gccaccagtt ccggaaatcc atccaaaacg gggttgtggt tttcgcttaa agcacggcgc
     2941 gaagagactc gcgcctcgct gtacttggcc tgcggtgaca aatctgatcc cctttcgtga
     3001 caggcctgcg cgaatgccac catagcggca tgaagttgtt tcatctggtc ggtgtagggg
     3061 cggcctcgga gctccaccag cacttgaatg gcaaccgtta gggcgaacca gagcagcttg
     3121 atttcgctag ttggtagctc tcgggctgtg cacgcagcag tgagtgcggc gatctgggat
     3181 gtgatttcct ctgcgctctg cggccacggg cgtgtggcga tcaactcgcg gatcctggtc
     3241 aagccgtctt cgatagcgtt ctcccaaagc agtttttccg tggggcagaa caagcgcacc
     3301 gtggcgtact gatcctcgaa accaaccaac agaagtggct gtggcccaac cttctccaat
     3361 cctcccagga ctgctcggat gtcatgttga agccagcagc tctccagcca gcgttcaagt
     3421 tcctccggcg gacatccgct ctgcgcgata aactccgact cctcgaacat cgagaacgcc
     3481 cctggcccgc cgagggcgac cgagagtcgc tccttgtcct cgtcactcaa tggaataggt
     3541 attagtttcc cttcggtagc cacgatccct cctccaataa cctttgtccc cgcgctactc
     3601 ccgatctgat tgcgggcgat cagcatccac tacacttgtc tggacgcgaa ttctccgcct
     3661 ggagtatcaa acttgaatcg acaggccacg atcaggacac tgaccggaac agacgcggtg
     3721 ggcgcggggg tcaaacagac cagaaacagg cgggtatcac ggcggaaaca ggccagccgg
     3781 tccccctagg atcgatcatc ccagaagatc tcggggcagc gtcgcctctg ctactttcgg
     3841 acggccttcg gcccctttcc tcacaagagg aggaccaggc ggtgttacca gagaatttag
     3901 gcggcaactt gtgctgattc aggtccgcct ttaaaggcag ggggaatacc caaaccctca
     3961 ctatctataa acgcggaaaa cccttcagca gttttagaaa tctcttttgg cccccagcgc
     4021 gcgatgcccc gccagcccga cttgtgccgc gatttgatgc gtgcagcgga caacccaagg
     4081 ccagtttgcc taggtttcgg gcttctctca agcagtaggg gaccagctga agccgaaagt
     4141 ggtgccccct cctcgcgcac agacctcgcg cagacagaaa tgtcctcctg gtcccctgct
     4201 gcttgcagga gcccgaagtc atcgaggcgg gggcgtcgcc atcaagttcg ggctactcgg
     4261 ggccgcgatt gctaccgagc gcctgaccct cagcagacac tccgaggccg cgaacacggc
     4321 gttcgtggcc aacctggcgg ggacggccgc actcacggcc gtcgccgtcc ggaactaccg
     4381 cttgcgaggg gcttagggat acctccgggc gagcggaggc gcacggtgac gcgcacgccg
     4441 gttgtgctgg cgcgtcgtct ggcggaggcc tggtgccgat tcgcccacca gggcatctac
     4501 tggcccgtct gcgggcagta ccgctgcaag gtctgcctgc tgacgcacgc ggtccactgg
     4561 ccgtgaaacg tttggcgtgc ggaacgtcac tattgagatg ctcgcacgca gccgcgccga
     4621 tccaatgtgg tcgccggtga cgggtcggcg cggtccttct tctgaaatct caacctgccc
     4681 ctgcgtcttc ccccaaaaag tggcgactac attaatagat gcgcgccgcc atctatgcac
     4741 gggtctccac ggttgaccag cactgcgaga tgcagctccg cgagctgcgc gagtactgcc
     4801 agcgccgggg ctggacgatc gccggagagt acgtggacgc cggctggagc ggtgccaagg
     4861 ccagccggcc cgaactggac cgcatcatgc gggacgccgc agcgcatagg tgcgacgccg
     4921 tgctggtctg gaaattggat cgtttcggcc gcagtgttct gaatctctcc gaacagcttc
     4981 agcgcctcag ttcgcacgcg atacggttcc tcgccatcag ccaggcgatc gacaccgacc
     5041 agtcgaatcc aacgagccgg ctcctgctgc acatgctagc ggcggtggcg gaattcgagc
     5101 gcgaactcat caaggagcgg gtgaaggcgg gcatggccgc ggcgaagcac aggggcaccc
     5161 gcatcggccg gccaacggtt gcactcgacc agaggcgagc ccggaagctg cacaaatcgg
     5221 gcttctcgca gcgcgcgatc gcggaggcgc tgggagtagg aaaaggcacg atcgcgcgac
     5281 ttctggcagc ctaaaaaacg gtcaaaaaca gtggtccaaa accctctctc gccgttgccg
     5341 gtttcacggc tctaaatcgt tgatttcagg ataggggatt ttttcgggct tacggtccaa
     5401 aacctacacc ttttgggcca aggcggggcg ggacttaaat tcacggaagg cccggaaaca
     5461 ttggggttgg agcgcatttc ggtgttgcgg cgacactgca ttccagttac gtcacaactt
     5521 cggacgaggg ccttttcgtg cgtcttgagg ccgccagcgg cccccgtttc gtgcaacata
     5581 agtctacacc cgaagggggg tcgagaaaat tgttgatgtg tgtgcgacgc gcgccctcgt
     5641 gcgccgctcg ttgaacgcct gctacgcccg ctgcgtcgcg ccgcggagac ctctaccccc
     5701 cggcccccag ggcgccgcct cggcccgggc gtggtggagt aagggaaagg gtcttccaca
     5761 cggagatccg ccccaagacc atccaaccgg catcgcgcat catcccgggt cggcttcctt
     5821 ttgtggtatc actccagtga tacggcatgt cggaagcaca tctgaaggca gagattgaac
     5881 gtctccgagc ggagaacgaa gcgctccgca agtcgccctc tcgggggcaa gtcacgctga
     5941 aggtcagtga gaagggtgct gtgtcggtct acgggctggg caggtttcca gtgaccctct
     6001 acaaggagca gtgggaaaag ctgctggcga tggctgattc gattcggacg ttcattgccg
     6061 aaaacgacca tctcctcaaa aagaaggagt aggcccaccg ccgccagcat ggaaccgacg
     6121 ccactccgaa cgatcgaggc cgtcctcgac gagttcctca ccgagcagcg cagctgcctt
     6181 tcccccaagc gagcgactga ttacgaggac atcattgagc tgctcaagcg gttcctgaac
     6241 ggatactggc cgggccacga ccaggctgaa tatgaccgca ccactgccgc cggcggctct
     6301 ttctgcacgt cattcggccc cgaggagatg ctggaagcct acggcgagtt cctgggctac
     6361 tacatgactc ataaggtcat cgccggcaag gagaccctga ggctggcggg aacggtgacc
     6421 aagaaactgg ctgcctggct cgcggagaag gagtacgtcg aagacgtcga ggaagcgcac
     6481 gagcgggcga aggaagcggt gagggaactg ccagtggcca ctgacgcact ggggatcctt
     6541 cgcaaccatg tggagaaaca gccaccgcga aagtgctcgg aggaaattca ggatcatttc
     6601 accgttgctc atgtgcagcc cggaagactg tggctcaccc cgctcaccga gcgcccggac
     6661 atcatcggtc cgattccgat tccggaagag gcgagccgcc tgttgcagga acactgggat
     6721 attagcgggt cagtcggccg gactcgtcaa gggtgggtgc tgcttgaggt gtggaacatc
     6781 tcaccgtgac gcaggtgcaa cgggaaagcg tcacccacac ctcgaagctc tctccacagc
     6841 cgccgctgct tgctcccgtc ccgcgaacat aagtccacac cagatcgggg ggtcgaaaaa
     6901 attgttgaga tgcgaagccg ctaggaccgg gcgaagcccg gaaaccttgg gaggcaacct
     6961 tgtttttctc gaccttggcc aacaacgctg ggccgtctat gcccaccttc agcccggctc
     7021 gctccgggtc aaacaaggcg acagtgtgcg gcggggcgac gtaatcgcgc gccttggcaa
     7081 ctcagcctcc tactcagccc acctgcactt ccacatcgtg gatggaccgg atcccttcag
     7141 ttctgaaggt gtgcccttcg tcttcgacga gttcacgcac gaaggcacgg tgcatcggga
     7201 tgagatgccg gcaggaggct ggacgattcg cttcgtggaa cgccaggtcg aacccaactc
     7261 tgaatttagg cgcttacccg cgagcaacgc cgtagggtag acctacgaac ttctgctcca
     7321 gaacggccgg ccactcggag actatcggcc acagttttca accacacgat cccaaagtcg
     7381 gcaagccgca catggggcct tcgctccttc gggcagcatt cattccgcga acaaaagtcc
     7441 acactcgatt gggggtcgga aaaataccag ggtgctctgg atttgcatcc tctgcagtct
     7501 cgtgttgctc tggttgtaca gaaaagtgaa agtcagtcca gtgatcgcga tgacgatctc
     7561 aacggtaacc ttgacccggc cccaccagtc cttctctcca gttttctgga cgcctttgcc
     7621 actgcttcca agtgaacctt ctggttaccc cgcgagtgcc gatttctcaa gatccttcat
     7681 cccgagcgac tccactagcc cacggatggc catctctgca tcttgccggt gaacgaagcg
     7741 ttaaactgcc cggatgagga gagagaagat gcgacatatt ctgagtgtgt tgttcgcagc
     7801 agccctattg ccggtgaccg ccgcggagcc gcccggcttc gtgttttgga agagcaccga
     7861 tctgaaatcg tacacggaaa aactggcgcc gaaggtgaat gagaagaagt tcgccaacga
     7921 gcagattgcc tcctggggaa accactcggc gatgatggtt taccgcaagg gagacggcga
     7981 agccgaggtg cacgagaaga ccgtggacgt gttcgtggtt caggagggcc gcggcacgct
     8041 cgtgatcggc gggaaagtcg tgggtggaaa gcagaccggt cccggtgaaa tccggggcgc
     8101 ctcgattcag ggcggtgtaa acaaaccgct cgcgcctggc gatgtcgttc acattccggc
     8161 gaacatcgcg catcaagtcc ttgtcgctcc gggcgccgag atcacttact tgatcctcaa
     8221 ggtcaatatg tagcggtggg cagcgccctt gctgctatca aggaggtcga ggtagttggc
     8281 gccgccgtcg tgcctaccga aggtgaccaa cgggcaccaa gtagaggacg gcgtgatctc
     8341 tggggacggg gattgccttc tgtaactggg ggtcgtcgaa cgccccaacc ggcacgccac
     8401 ccagattcaa cgccaccgcc tgaagcagga ggttttgtgc cgcgtgtccg atttcgatgt
     8461 gcacatagcg tggggcgcgc gcctccccat atctttgtgc cgtgcgtgca ctgaccgcag
     8521 tgaacaaaaa gaccgcggcg gcgtcggcga tcggtttctg tccgagtgcc gccttagaaa
     8581 tgatcgttcg cagatcggct tcggccagcc gttgcagttt atggccgcgc ggctcgtagt
     8641 ggaagaaacc atctcgggtg gcaacgtaga gttccagtgg gtaaagtgcc ccggctgagg
     8701 gagccgtccg cctgctccca ccgtgggtta tcccttgagc tgcccacagc agttgcgaga
     8761 gctcctgttg gtccaaggcc cggttcgcaa actcacgcac cgacactcga cgggcgagtg
     8821 cctcctgaag cggcatgccg cctgcacagg ccggagctgg gagggccacc tggtttcccg
     8881 ttctggccga acgtttcggt tcctccccgc aggcgatcga taccatcgcc acaagtagcc
     8941 aaagggcggt tgccaggaat ctgtcgttag tcatgctttt gccccggatg tagcgtatcc
     9001 ggcctgtgtg agcgcagtca atgcctgaca tgtcgtccaa gtggatgtgg acaaggcgaa
     9061 cacctcgatt aagagcgtcg acgccggcct gtccagcatt gagcagacgg ccgcccgggc
     9121 ctcttaccca ggttggcgtt acggagcatc tcccccattc gctcacgctc gtctatgggc
     9181 cggcgctcgc cgagggtgga ggcgcggtaa agctcgatga cctgatccat gtcgaggttg
     9241 tttccggtgc gataggtgat catggagtga agccgaggct tcctccactt tacttcggcc
     9301 ggcgtcctcc ggcttcagca gtgcaggtac gggctctgct cggtggcgac cgctcgagca
     9361 gccgtgcgga acgcactacg cgcccgcgcc ggcggatcag cgccgccggc cggaagcgga
     9421 tcgctgaagc ggctcggaaa cgcggggcac aggcgagggc gaagaaagcg tcgagcgctg
     9481 cctaatagcg tgcggggcca gcgaagccgg ctcgacgacc ggccgcgagc gcgccctatg
     9541 tcgtgtcctc caccgaggcg ctccgcatcc tgtcgaaggc cagccgtgaa ttgatgcgta
     9601 actacatccg cgaaaacaac cagaccccgc gcctttctac tgggtagcga ctgccaacac
     9661 catcgttcgc aaggtcagga agtataaacg aactccagaa acaggagact aggcgtccgc
     9721 ttcctcgcgg ccgcgcaggg tggcgttgcg gatcagcggt tttgcccaga acccaacgct
     9781 cagtacccag cccagcgaga gatagagcac cagcatcccc gcccgcaggc ccagtaggtc
     9841 cccaaggtgg ccgatcacgg gtggaaaagc cgccccgccc acgatggccg tgcagaggat
     9901 gccggcgaaa actccgtggg agtgatcgag cgagttcagc cccagcgaga tcatgatggg
     9961 ccacatcacc gaaaggaaga gcccgctcac tgggaaagcc accagggaga cggacgccgg
    10021 tccggcgatc gcggctgtga aggcggccgt ggcgcccagc gtggtgccga tcagcacggc
    10081 gcggctgtcg aacagcttga gcagggcccc tcccgctagg caccccagcg tgaggctgcc
    10141 ccagaaccag gagaccgcca gcgcgcccgt ggtgcggggg tcgaagcgat gatacgtgaa
    10201 caggaactgg gagatccagt tcgccaggcc ctgttccagc cccacgtaca ggaacatggt
    10261 cgcgaagtag agccacacca gcggcaggcc tagcagccgc cggtagttcg cccacgcgcc
    10321 ggcgccctct tccccatgga tccgccgggg cagccgcacc gccgccgtaa cggccagcac
    10381 caccaggccc gcgggcgcca tcacccagaa gatcgcgatc cacggcagtc cggccggggt
    10441 gagcgcccgg agcgggtcca gccagaagct tcccccactc tcctgcccta cccgcagcac
    10501 caagtaggaa tagagcaacg ggcccaggaa cgaccccgtc ccgaacgtga actgcgccag
    10561 ggccgaattg aatgcgtagt gctcctcgcc gcccgcgatg cgcagcaggg ggttgatgac
    10621 cacctggagg gcggccgccc ctgtccccac cacgagaaac gaggccagcg ctgtcgcgta
    10681 agtcggccgt agtggcagca gtaacgcccc ggccacttcc aggaccaggg cgccgatcac
    10741 caccgtcttt acggaggtcc gctccatgac gatcccggcg ggaatggaaa ccagcccgta
    10801 agccaggaag aagacgaacg gcagcagtcc cgctgccgcc aggctgacgc ggaaaccgcg
    10861 gatgatttcc gggatgagcg gccccatgat gttcgtcagg atcgaaatca ggaagaacac
    10921 caggaagacc agccaggtga gcttccggct gcggccgcct tcgcccgacg cagtcaaagg
    10981 gtccctccga ggcgacgcat gacttccagg caggcgcggg tggcgtgata gggtccccgc
    11041 cacggaccgg ccttggtggc gtcgatggtc ccgtcgggct cgatggccgc aaaccagtcg
    11101 ccgtactcgt ggtcggtgaa cacgtccagg acgtaagcgg actggcgctc gaaggcctcc
    11161 ctgtaccggg cgtcgcccgc cagttgccag gcgttcagcg tgcccaccag ggcttcggcc
    11221 tgctgccacc actccttgcg ccggcagtcc gccgggccgg cggctgggcc ttcgagatac
    11281 aggcccccgt tttcgtggtc gaagccgtcg cgcagggcgt ggtccaccag ggacagggcg
    11341 gcgcgccgca ccagcgggtc ctgcggcctg ccgagcagtt gcgcggcatc ggtcatgagc
    11401 cagctggctt ccatgtccag gccgtacagg cagcgcaggg catcgccgtt ccagtcctcg
    11461 ggttcccact tttcgttgaa gtacaggcgg cagtatccct ggcgcggatc caccacccgg
    11521 gcggcgaaga tctccagcag ttcctccagg cgctctgcgg cggcgcggtc gccggtgacg
    11581 tcgtaaagcg tggcgaaggc ttccagcagg cccaggtgcc agtccgacga tttgcggccg
    11641 tgcgaatcgg ccagcacggg gtggacgtca tctacgggcg ccacgcaggc cgtgaccgag
    11701 acacgcgggc cgctccggta ctccaggatc ggctgccagt tttgcgtgaa cgcgacccgg
    11761 tagccgctgt gggcgccgtc gtgcgcgtgg cggtccatca gccggaacag gtccagcgcc
    11821 tcctccagcg cgccgtcgtc gcaaaatgcc tggccgtatg cggccagagc ttcgagcacg
    11881 taggccatgg gattgagcag cttcatcccg tcgagcaggt tgccctcgcg gtccacgagc
    11941 cagtagtagc cgctgtgttc gggatcccgc atcttgcggc gcaggaagtc gagcacccag
    12001 gccgcggcgt ccgcgcaggc gggcgtggga taacggcggt agatctggca caaagcccac
    12061 aaaagacgcc cctgctgggc caccgacttg gtgccggggc cggcggggcg cccttgccgg
    12121 ccgagccagc ccatggctcc cccgtactgt cggtcgatcc cgtagtccag ccagaacctg
    12181 agaatcccgt ccaggagatt cccgcggaat tccgccatcc agtcgcgcct cgtcatgcct
    12241 cctccttgcc aggtcccacg ggaaactagt ttagtatccg tacggactcg tcagagccgg
    12301 gaacactcag aaaaaaggac aagttccttg caccaatgct acggtctggc agaggccccg
    12361 gttcaacaac accacggcgc acactgggcg gtagatgtag agtgctttgt gattgaacac
    12421 aagagtctcc tcacgctcgt caccgagaca actcaggtgc tgcacctgtt cctcgggaca
    12481 gagatcctta ccaaggcaca ccaactcctt tgtgctggct ttccaatgcg agaagcctag
    12541 cagccacttc tggaagcgag tccgcactcc cgaccagagc gcttatcgcc tccattgccg
    12601 ccacatggaa gccagttcgt ttggacggtc aaaatatcaa aggatagcgg atcggtatac
    12661 actgaactgt gctgggggcc agaatggaat gctcatcagg ctcggcagaa attgctgggt
    12721 cgcaggagga gggcgtaatg acactttcac gttcggtcaa aatgtttcct ctgtacacgg
    12781 tggttgtggt gggggcaacg tgcctggtgg gcgcggctgt cgctcaggga ccagcggaag
    12841 acaatctgcc attcacatta aacggcaaga cgtgggtcag caaaaaggcg ttcattgaca
    12901 gtggaggccg atgtgcaacc cgaaacgtcg attcgatcga agcagaccag atcgaggccg
    12961 cgctagcacg attccttgcc gccgccggtc aaggttcttc cacgaagccg ggaaagcccg
    13021 gcggaggccc accaccaccg gtgacctcgg tagtcgtttc ggtctatttc cacgtgatca
    13081 ataatggacc cgggatcgaa aacggcgatg tgcctcaatc tcaggtcgag ggccagattg
    13141 ccgtcttgaa tgctgcttac gccgccgaag gcttctcctt cagcctggtc tcgactgatc
    13201 gtacgacaaa ttccgcctgg tacaacatga cgccggggtc gcaggcggag cgacaggcga
    13261 aggcggcgtt acgcgtgggc ggagcggatg cgttgaacat ttacactgcc aatctgggta
    13321 gtaatctatt gggctggtca acgttcccat ggggctatgc cggcgatccg accgatgacg
    13381 gtgtcgtgat cctttattcg tctctccctg gcggcacagc tgtaccatat aacgaaggag
    13441 acacggcaac ccacgaaatc gggcactggg cggggctcta tcacacgttt cagggcggat
    13501 gcaacacgaa gaagggcggt gacctgatcg ccgatacgcc tgcggaacgg tctcccgctt
    13561 acggctgccc caccggccgc gactcatgta aggcagatcc cggacttgac ccgatctaca
    13621 atttcatgga ttacacagac gacgcctgca tgtttgagtt cacgacagat cagggaactc
    13681 ggatgggggc catgtggacc cagtaccggc aaccctagtt ctgtgactcg agcggcggca
    13741 gccagtcgat ggtgagctgc gggcggccgt cggctttaca ctcccagacg gtcgcgcgca
    13801 ccccgggccg gacccggccc tttgtggact caatcgcctt ccagtcgcca ggctggaatg
    13861 tattcgaacg cagtagaaac ctgcccgccg ggttctcacc ttctgcaaaa cgcagcgtaa
    13921 attcctcgtc cgtgtcccag tctgtgcgga tgcggatttg aagttgctcc tcactgcgac
    13981 tgtacgaggt caactttccg gtgtacgagg tgaggtggtc tctcgggcac tcaaccgctc
    14041 ggggaggacg ctggcggccc ccaccgcggt tttggggctt gcttgaagtc tgcccgtagg
    14101 cgccagtaaa cactaaagca agagacagga tcgctctcag cataactgcc cccgctcctc
    14161 tacccattct agctcggact tctctcggca atctgacagt acccccgaca ctgcaaattt
    14221 tgaggactgt tgcgtaggca aatggctgtc cgatttcgac acccgccgca ccagcacggc
    14281 gttttgtagt gctccgttaa acggccaacg cgtgaagggc acagaaaact ggaggcatgg
    14341 cagacgtcag tggtgcgcac tctgaaccgc ctgctccgac cggacgagtg gggcttcgag
    14401 agctctcttg gctgaaggct cctgttggaa tgtccgaatt cggatgcaag tgttttgcgg
    14461 gcaaggtcga gactgattct ccttcgcgaa gggagttgga gcttcaggaa tgctttccgt
    14521 ccagtgccca cgagccgcaa tcgaccagga acgccttcgc tttggccgcg gactatccga
    14581 accgaaactt cctgcaagct gccagcggct tggatttgcg gcgacgtctt tcgtcttcga
    14641 gttctctggc cgcctgctca tggactccag gtcgaagtag atcatctcat ccaggatttc
    14701 gttgacacgt tctgtaccct ctctccgctg cttggccgaa ccaagagagg gctgccgtag
    14761 aaagagtgcg cttcggagga tcaagacttg aatgcttgtg atagactcat caactcgacg
    14821 agtaacggcg atgcggaacg cggtgaagcg caacttcatt cttcaacagg ggttcgagaa
    14881 ccctgctcct gcgctctgcc cgctccagta ttctgcgctt ctatttcagc cccgaatcgt
    14941 gttcatctgg gtcgttgccg gagtcctgtt ccagtcccca gccgtcttcg cggccctctg
    15001 cgctgttctg tggtggagtg ctctgtttcc gaaactgaat ccgttcgatg cgctgtacaa
    15061 ccggaccatc ggcagccggc cgggggcgtt tcgcgttggc cccgccccgg cgcccagacg
    15121 cttcgcgcaa acgatggccg gagccttcgc gctaaccagc gcgctgctga ttcacgccgg
    15181 gttccgtctt gcggcatacg tagttgaggc gattttttcg gcagccgtgt tggcgctcgc
    15241 aatcgggggc ttttgcctcg gctcgttcgt ctatcacctg ctccgcggac atgggaggtt
    15301 cgcccgtcag accctcccgt gggcaagcta gtgatcctcc gggagctggc ggcacacccc
    15361 gccgagtgga tgccctggaa ctaccgccag acattgccgc gcatgtgaag taagatgaac
    15421 cctcctggcc gaaaaggatc gacggcggcc atggttgagt gaacactccg aaatggtcgc
    15481 caaacggccc tttgcagagc caaaatcggg taacgttttt ggccgggaac gcattggcga
    15541 ctggcacgac cgggcaccac agggccactg tctctgacgt aatttctgcg cgccccgaaa
    15601 gctcccggtt ggcttcggta acgtcctccc ctgggagcag cggaggagca acgccttatc
    15661 gcgggtccct tcccctgggc ttccggacta accggacatc cgccttggtg gtccaaaacc
    15721 cacgcttttg gaccatcagt ccctgatgcg cccgctgaag ccgaaagtgg tgttcctatc
    15781 gcgagcctca aggcgcacga tgacgaacgt ggggcggttc agcctctgcc gggaacaact
    15841 ggcgacccca ccgcgtctta tctgaacgag gcaagacgcc ggcatgccat ccctgctcga
    15901 ggatatccgc tactccgtcc ggacactctg gaagaagccc gcgaccgccg cggtggtgtt
    15961 cttcaccctg ctcgtgggca ttgtgttgaa tacatccgtc ttcagcatct gcaacgtgct
    16021 gtggttccgt cccctcccct tcaagaaccc cgaccagctt gtcgtgctgc ttcaaggcaa
    16081 tccgcgccag ggctcggaac ggctctgctc gcagcgcgat ttcgactttt tgcggcagca
    16141 tgcccggacg gtcgaggcgc tctccgcagc aggcccgcga ggcttcaacc tgtcttcggg
    16201 ctggccgggg ggaccggagc cccgcgacgc ctcgggagcc gcggtgacag acggcttcat
    16261 tgagaccctc gggtttcgtc ccatcgcggg acgccttttc acgacggccg actttcaagc
    16321 cggagcgccg caggtggttc tgatcagcga gcgcctctgg agaacctggt tggccggggc
    16381 ggacgtggtt ggctcgcagg tttccgtgga ccgggagaag gcaaccatcg ttgggatcct
    16441 gccccgcaac ttccgctttc tcaccggtgg atacgacgtg gtgacgccgt tgactcaggg
    16501 acgcacccag cgagaggtcg aggccaataa cctcattgtg ctggccagga tgcgcccgca
    16561 gcaggcgccc cccgctatgc aagctgagat gactgcgcta atgcgggaac ggttgacggg
    16621 ggaggccggc tggggcttgc gcgtcttccg gctccgcgac cagtacatgc gcgaagctcg
    16681 catcctctac cccgtcctcc tggcggcagc ctggctgatt ctgctgatcg tgtgcgccaa
    16741 tatctcgaac ctattgctgg cgcgctctgc ggcgcgcagg aaggagatgg cgttgcgcct
    16801 ctcgctgggt gcgccgcgtt cggagccggc gcggctggtc ctggtcgagg gtgtgctgct
    16861 ggctgtgctg gccggcgccg cggcgttgcc ggtctgcctg tggatccgta atgcgctcgt
    16921 tgcggccgta ccggacatgg cggagatcgt tgtggactgg cgagttatgg ggttcacctt
    16981 gctggctgcg ctgctggccg ggctggtctt cgccatcgga cccgcccttt cggtcttgcg
    17041 aatcgacgtc aacgctgcgc tgaaggagga aggcgccggc gtttcccggc gctccgcttt
    17101 caggctgcgc cgggccctgg tcgccgggga gatggccgtc gcgatggtta tgctgacggg
    17161 taccggactg ctcctgaggg ccgtggcggg aatgttgacc gtggatgccg gcttccgtag
    17221 tgcgggcacg ctggtggcca gagcgaatct tcctcccgcg gtgtaccccg acaccgcgca
    17281 tcgcgcggcc gccttaaccc gcctgagcga tgcggtgcgc gcgatgcccg gagtggactc
    17341 cgtagcggtc gtcagttcca cgccaatgca gggagagatc cgccggttgc gcttggaagt
    17401 ggcagaccgc ccggctcccc ccgaagggga gacgatccga gcggcgttga catccgcggg
    17461 ccccggctac ttccggactg cgggcgtgcc cctcgtcagg ggccgggaat ttacggaaca
    17521 ggatgacgcc ggcggtccgg gtgtcgcgat tttgaaccgg acgctggtga aggcgctgtg
    17581 gggcgagggc gccaacgtcc tcggacagcg gttacgcacc gatcccaagg acgactggct
    17641 ggcagtggtt ggtgtcgtgg gcgacacacg gcaagacctg atgcgcgagc cattcccgga
    17701 agtctacgtt ccctaccggc gaatctgccc accctccgca acgctggtgg tggggaccag
    17761 gctgccgcct gaagcgctcg cgggcccggt ccgtgcggcg ctgcgggcgt tggacccgga
    17821 gctccccttc tccggtttgc ggtccctcga agacctgcgg cgggactaca tcccccccgt
    17881 ctataccatc atgctggccg ccttcagtct actggctctt gcgctggtgt tcgtcgggct
    17941 gtacggtttg gtctctttct atgtcgtcga gagcacgcgc gagatcggag tgcgcatatc
    18001 gttgggtgcc ctcccgcgga acgtcctggc attggtggtg cgccaaggcg tgcggctggc
    18061 ggtgatcggg gtgctcatcg gcttggccgg ctcgatcgcg gtggggaagt tgctctcggg
    18121 cctgttgccg accgtcatac gaccttttga cgccggcctg tttctggccg tgagcgctac
    18181 gcttctttcg gtcgccgtcg cggcatcctt gatccccgcg cggcgcgcga tccgcctgga
    18241 cccggcggcc aacttgcggc acttctgatt tgcctgtggt tctcgaaaaa cccgatctgg
    18301 ttgataaagt gggtgttgcg cctctggcgt cgattctcgc ccgctctcgt attctcagcc
    18361 ttgtttgcgt cccttttggc gtcattccgc agccgtgcag cgctccaaat tgagctccat
    18421 tccgcgaaca taagtccaca ccagatgggg ggtcgaaaaa attgttgatg cgtgtgagac
    18481 gcgcgctctt gtgcccggcg aggatctccg ccccctgtac ggtgtagtaa ggaaaagcga
    18541 tcgtaggcgc ctgaccgctc tggcggccat cggcgtcagc gccgccgtcg ttgtgcaggt
    18601 gctgcgaccc gaggcttgct gatacactcg acagtttcgg acccgatgca tgcagaacgg
    18661 caactgtaag acgtcagcgt cggtcccact tcacgcacag gagaccccat gccccgcgga
    18721 aaacgactag accccaggat tctcgaagcc gccctcgtcg gcctcgaagc ccaacggcaa
    18781 aggacagatg agcagattgt gcaggtacgg gccctgctcg gcggcgaccg ctcgagcagt
    18841 cgtgcggaac gcactacgcg gcctcgccgg cggatcagcg ccgcgggccg gaggcgcatc
    18901 gccgcggcac agaggcagcg gtgggcgcag gtgaggcagg ccaaggccga agggaccaag
    18961 cccaaacgga gactaagcgc cgccggccgg aagcgcatcg ctgaagcggc tcggaaacgc
    19021 tgggcacagg cgagggcgaa gaaagcgtcg agcgctgcct aatagcgtgc gggg
//
LOCUS       DATIFQ010000459         7975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_347173, whole genome shotgun sequence.
ACCESSION   DATIFQ010000459 DATIFQ010000000
VERSION     DATIFQ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347173"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="VLE22_23065"
     CDS             complement(<1..1413)
                     /locus_tag="VLE22_23065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17349.1"
                     /translation="MTDRRNFIHTSILAAMASRLSAQDTPVTHPRVLLTPELLHEIAG
                     KVRGPFAREYKTMLETAIQGPKGMDQQYAVPVAFMEAGLAWLVERELGRDGRPYAEKV
                     LEMWSQPAWQTPGMTRHFGWQGLLYDWLYDAMTAEERLKFGELLGEWVATWWKTAEVN
                     IPRDGWWYNQHWGPVHLDVPHNRVALTSKLFMSLAAAGNAGRFEEAVRTNLKSFDEKF
                     RAEGLPALDEMGGVWSESNGHGGYGPLLVIPLSYQAAKTGLGFDPFAASAAEGFTREY
                     IQACIYSLMPHSSKMAYIDDCNSGYPVQQARCAPLFARHYKDGVARWLSDTALERGWL
                     FDKRTGQDEVWQRIAFLPAELKPATPKESGWPLARHFRGAGHVYMLGAWDDPNATWAF
                     FGAGPTYAGHSRDDEGHFLISKCGHLANRSGGAGHNDGCYYSGGSLIYNIVTVYHPEE
                     KMRRNAYNENDGGLIRHVYEN"
     gene            1462..1710
                     /locus_tag="VLE22_23070"
     CDS             1462..1710
                     /locus_tag="VLE22_23070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17350.1"
                     /translation="MRPKEIDDVLINPGIGFMTFHKANGGPINPTNQGQPETSMDYLR
                     TYWNFFEPRRGEYRWDVIESALRVAHAKRQTLMFRIMS"
     gene            1909..2241
                     /locus_tag="VLE22_23075"
     CDS             1909..2241
                     /locus_tag="VLE22_23075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17351.1"
                     /translation="MGAWGEGAGSADLSQPRREALVDCYLETFHKTPLVMLLTDEKTN
                     KYGISRRPVGWRVDCLSDMGGFSPTWSHMFDYYPQGIINFGMEDAWRKAPVSFKACWV
                     MNHWKKNG"
     gene            complement(2633..4084)
                     /locus_tag="VLE22_23080"
     CDS             complement(2633..4084)
                     /locus_tag="VLE22_23080"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HSB17352.1"
                     /translation="MRTWLRVLAGAVCAAALSAQPQQKPLREHPDVAGAVRLYESWVT
                     EQMTYRGLPGVAVGVVHDQQVIWSRGFGYADVAAKTPVTPATIFRMASHTKMFTAIAI
                     LQLRDAGKLRLDDPVSKHLAWFHIKPAAEDDPPVTIEELLTHNSGLPREATSPYWATF
                     EFPTEEQIRQTVNSQQATYPPQAIWKYSNLAFALAGFVVEAVSGEKYADYVDRHILRP
                     LGMTNSSIEVPSGRMSRLAKGYGRRMPDGSRKTMPFSDTRGVGPAAGLSSTLDDMLKY
                     ASFQFRTGKAGGSQILSGATLREMHRPRVLRPKWVGAWGLGFSVQRQDDKIMVGHGGS
                     LAGYKTHTLVNLDDKVGVVVLSNGDDTRPEQFAQRAMKIIGDAIAKAAKPPEKQKTWD
                     PKWSKYSGLYRTIWSDTAVVEMNRELVIIDPLTDDPQEGLLRLVPQEGGVFKLEGPAG
                     FGAAGETVRFEEDSQGRVVRMFVGELPAERVEK"
     gene            complement(4102..5625)
                     /locus_tag="VLE22_23085"
     CDS             complement(4102..5625)
                     /locus_tag="VLE22_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17353.1"
                     /translation="MTAPFGCGLVLVLLAVANTCVVETRERPRPPAPAPEVEVTLQAR
                     LAAPGRETVSFGVPLPPGYLNDTRFVSLRDVGGREITVSVRPLEPWRIDGKDGSIRSI
                     LVQFTADFSGGRTRRVKVVFGRKRRKDCVRFVPVAATLLDPDGLDGPRVLAVLPAKWL
                     CDSWIAGPQVPASESGPFAAYDDFVEHSFPGALQYKDSQVYDHWLFDRTACWYKMYIR
                     TGDPKYLEAAYHAAHFVRLHTKMDGPNAGIFTLKGVDLKYVYPRAMHLHYLLTGDERM
                     LEAGRIMARFCLNNWDPKYTTDRRFWTTRHEGYGLLGVLHGWELTGDATYWRKAREYT
                     DALYNHQRQPPDGLPPDCSWRQNWQTYASEEARFRSGTSAWMTAILLDPLFHYWTLTG
                     DPRVPEMVSAWCDFLDRRGLVPDGSEAYYVINAIPAPGEPPGRRGHDMFLHNTEMAYM
                     FAMGIYFSNDPERAEAWSRRFDTLFRSALKQDINRTVRAYNWAFQASSQMVYFLKSR"
     gene            complement(5683..6996)
                     /locus_tag="VLE22_23090"
     CDS             complement(5683..6996)
                     /locus_tag="VLE22_23090"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17354.1"
                     /translation="MTRRNFLATSGTALAAAASPLATGIAGAQGKKLKVATVGTGSRG
                     TLTWGIPVVKGYSDVVEFVGLCDINSKRVKAAQGMMGISAPTFTDFDEMVKKTRPDAV
                     IITTVDGTHWRYIVRALELGCDAISEKPMCTNEEQCQAILDAAKRTGRKVTVTFNARH
                     GREDKRVKQLLMEKAVGDIVSVDFHEYLDTSHGADYFRRWHYLKENSGTLLVHKASHH
                     FDQANWWLNSTPTEVSAYGELKVYGRNGTFRSTHCRVCPFKQQCKFYWDITKSERMKE
                     MYTDCESEDGYLRDACLFRENCNIYDTMSVRVKYENDVMLTYTANTYQPFEGQAIAFN
                     GTKGRLDANHFGGGGYSQGEVRLTRAFGKSEVIRDFEERREGGHGGSDTSIQDLVFRG
                     KPDPDPLGLRADLRAGAYGSLIGIAAYHSIERGGETVKISDLVKL"
     gene            complement(7127..7579)
                     /locus_tag="VLE22_23095"
     CDS             complement(7127..7579)
                     /locus_tag="VLE22_23095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17355.1"
                     /translation="MKANGIDGVLPMKDDAGIGSSPASHSAAVVSGVIGGLVVVLIGL
                     LAGKLGEPLSGNALFGPSVPALAAAMAAAVSAHRAGSSFGTALAVAIGAFLVAYVAAV
                     WFTHWMGVGGLVRRRPVNPVVLGWRRDLGVCFIGIAVGAFLGTFLGRR"
     gene            complement(7657..>7975)
                     /locus_tag="VLE22_23100"
     CDS             complement(7657..>7975)
                     /locus_tag="VLE22_23100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17356.1"
                     /translation="SVQTFLEKGARSYHGIYWWVPDMLTKGDQGDFAAAQAPRPLMLW
                     APLDDIGMPKEGVDEFLKVVEPAYAQAGAKTNLVVHRQPGQHEFTMEAFAAMKEFFDR
                     YFR"
BASE COUNT         1490 a         2566 c         2396 g         1523 t
ORIGIN      
        1 gttctcataa acatgccgga tgagtccgcc gtcgttctcg ttatacgcgt tccggcgcat
       61 cttctcctcg gggtggtaca cggtaacgat gttgtagata agtgacccgc ctgagtagta
      121 acagccgtcg ttgtggccgg cgccaccgga gcgattcgcc aggtgcccgc acttcgagat
      181 gagaaagtgg ccctcgtcgt cacgagagtg accagcataa gtcggaccgg cgccgaagaa
      241 cgcccaggtg gcgttcgggt catcccacgc tccgagcatg taaacgtgtc ccgcgccgcg
      301 gaagtggcgc gccagcggcc agccggactc ttttggtgtc gccggcttca actcggcggg
      361 caggaacgcg atcctctgcc acacctcgtc ctgcccggtg cgcttgtcga aaagccagcc
      421 gcgctccagc gccgtgtcgc tcagccagcg ggcgacgccg tccttgtagt ggcgcgcgaa
      481 gagcggcgcg caccgcgctt gctgaaccgg gtaacccgaa ttgcagtcgt cgatgtacgc
      541 catcttcgag ctgtgcggca tcaggctgta gatgcaggct tggatgtatt cgcgcgtgaa
      601 gccttcggcg gcgctcgccg caaaggggtc aaaaccgaga cctgttttgg ccgcctggta
      661 actcagcgga atcacaagca gcggtccgta tccgccgtga ccgttcgact ccgaccatac
      721 cccgcccatc tcgtcgagag cgggcaagcc ttcggcgcga aacttttcgt cgaagctctt
      781 gagattcgtc cgcactgcct cctcaaagcg gccagcgttg ccggcggccg cgaggctcat
      841 gaatagcttg ctcgtaagcg cgacgcggtt gtgcgggacg tcgagatgga ccggtcccca
      901 gtgttggttg taccaccagc cgtcgcgcgg gatgttgacc tcagcggtct tccaccaagt
      961 ggcgacccac tcgcccagca gttctccgaa cttcaaccgc tcctccgcgg tcatggcgtc
     1021 gtagagccag tcgtagagca gtccctgcca tccgaagtga cgtgtcatcc cgggcgtctg
     1081 ccatgcgggc tggctccaca tctcgagtac tttctcggca tacggacggc cgtcgcgtcc
     1141 gagttcgcgc tccaccagcc aggcgagccc tgcctccatg aatgcaaccg gcaccgcgta
     1201 ctgctgatcc attcccttcg ggccttggat cgcggtctcg agcatggttt tgtactcgcg
     1261 cgcgaacggt ccccgcacct tccctgcgat ctcgtgcagc aattccggcg tgagcagcac
     1321 gcgcgggtgc gtcaccgggg tgtcctgcgc gctcaaccgc gacgccatcg ccgcgagtat
     1381 gcttgtgtgt atgaaattcc gacggtccgt cattctcgcc tattgtgcca cggcgttcgc
     1441 gcggcccaac aggcggtgac ggtgcgcccc aaagagatcg acgacgttct gatcaacccc
     1501 ggcatcggct tcatgacctt ccacaaggcc aatgggggcc ctatcaaccc gacgaaccag
     1561 ggccaacccg agacctcgat ggactacctg cgcacgtact ggaacttctt cgagccccgg
     1621 cgcggcgaat accgctggga cgtcatcgaa agcgccttga gggtggcgca tgccaaaaga
     1681 cagacgctga tgtttcgcat catgtcgtaa cggaaccgac gcgtccaacg acgttccggt
     1741 ctggtttcgc gaaatgatgg gacctgagcc gaagtggaac aactcaaagt caaaggtgcg
     1801 cacagatccc gaggatccgc gctacctcca gtattacggc tgcatggtgc gggagatcgg
     1861 ccgccgctac gacggccacc ggacctcgac agcgtcgata tttcgattgt gggcgcctgg
     1921 ggagagggcg ccggctccgc cgatctcagc cagccgaggc gggaagccct tgtggactgc
     1981 tatctcgaga cgttccacaa gacaccgctg gtgatgctgc tgaccgacga gaagaccaac
     2041 aagtacggca tctccaggcg tcccgtcggc tggcgtgtcg actgccttag cgacatgggt
     2101 ggcttcagtc ccacctggag ccacatgttc gactactacc cgcaagggat catcaacttc
     2161 ggcatggagg acgcctggag gaaagcgccg gtcagcttca aggcgtgctg ggtcatgaat
     2221 cactggaaga agaacggctg aagaagatgg gctaccgctt cgtgttgcgc aagttcacct
     2281 atccggccgt cgtgcgcccg caaggaagaa tcgcgttcac aagctggtgg gagaacaaag
     2341 gcgttgcccc atgccataag cagtttcccg tggcgctgcg gctgaagaac ggggatctcg
     2401 aacacgtgct ggctaccgca gccgatatcc gtggatggct gcccggcgac aatctttacg
     2461 acgacgctgt gttcctacgg gcggagacac cgcccggtga gtacgatatc tcgacattgc
     2521 gatgctcgat ccgaccaccc ggcagccgaa gatcaggctg gcgatcgccg gcggtaagcc
     2581 ggacagttgg tactcgctcg gcaagatcac agtcgaggat taatggcctc gtctacttct
     2641 cgacacgctc cgccggcagc tcgcccacaa acatcctcac gactcgaccc tgcgagtctt
     2701 cctcaaagcg tacagtctcg cccgccgcgc cgaacccagc cggaccttcg agcttgaaca
     2761 cgccgccttc ctgcgggaca agccgcaaca acccttcctg cgggtcgtcg gtcaggggat
     2821 caatgatgac gagttcgcgg ttcatctcga cgaccgccgt gtcgctccag atggtgcggt
     2881 acagcccgga gtacttgctc catttcgggt cccaggtctt ctgtttctcg ggcggtttcg
     2941 ccgctttcgc gatcgcgtcg ccgataatct tcatcgcgcg ttgggcgaac tgttcgggac
     3001 gtgtgtcgtc gccgttggag agcaccacca cgccgacctt gtcgtctagg ttcaccagtg
     3061 tatgcgtctt gtaaccggcg agcgacccgc catggcccac catgatcttg tcgtcttgac
     3121 gctgaacgga aaagcccaga ccccacgcgc ccacccattt cggccgtagt acccgtggcc
     3181 ggtgcatctc gcgaagcgtt gctccactca aaatctgcga gcccccagcc ttgcccgttc
     3241 ggaactggaa tgaggcgtac ttcagcatgt cgtcaagcgt cgacgagaga cccgcagccg
     3301 ggcccactcc ccgcgtgtcg gaaaagggca tcgtctttcg cgatccgtcc ggcatccgcc
     3361 tgccgtaccc cttggccagc cggctcatcc ggcccgaggg cacttcgatg ctgctgtttg
     3421 tcatcccgag cggcctcaga atgtgccgat cgacataatc ggcgtacttc tcacccgaaa
     3481 ccgcctctac gacgaagccc gccagcgcga atgccaagtt cgagtacttc cagatcgcct
     3541 ggggcgggta cgtggcctgc tgcgagttga ccgtctgccg gatctgctcc tccgtcggaa
     3601 actcgaacgt cgcccagtac ggcgaggtcg cctccctcgg gagccccgag ttgtgtgtca
     3661 gcagttcctc gatcgtcacg ggaggatcat cctccgcagc cggcttgatg tggaaccagg
     3721 cgaggtgctt cgagacgggg tcgtccagtc gcagcttccc ggcatcgcgc aactgaagga
     3781 tggcgatcgc ggtgaacatc ttggtgtgcg acgccatgcg gaaaattgtc gccggcgtca
     3841 ccggagtctt cgccgcgaca tccgcatagc caaacccgcg cgaccagatc acttgctgat
     3901 cgtggacgac gccgacggcg acacccggca gtccccgata cgtcatctgt tcggtcaccc
     3961 agctttcgta cagccggacc gcgccggcga cgtcggggtg ctcgcggaga ggcttctgct
     4021 ggggttgggc ggacaacgcg gcggcgcaga ccgcgccggc caggacacgg agccatgttc
     4081 gcatcgatga acctcctggt ttcagcgact ctttagaaag taaaccatct ggctcgatgc
     4141 ctgaaacgcc cagttgtaag cgcgaacggt ccggtttatg tcctgcttca gggccgagcg
     4201 aaacagtgtg tcaaagcggc ggctccatgc ctcggcgcgt tctggatcgt tgctgaagta
     4261 gatgcccatc gcgaacatgt acgccatttc cgtgttgtgc aggaacatgt catggccccg
     4321 ccggccgggc ggctcacctg gagcgggaat tgcgttgatg acgtagtacg cttccgaacc
     4381 gtcgggcacg agcccgcgcc ggtctagaaa atcgcaccat gcgctcacca tctcgggcac
     4441 gcggggatcg ccggtcagtg tccagtaatg aaacagcgga tcgagcagga tggccgtcat
     4501 ccacgccgac gtgcccgacc ggaaacgggc ctcctccgag gcatacgttt gccagttctg
     4561 ccgccacgaa cagtccggag gaaggccgtc cggaggttgc cgctgatggt tgtacagcgc
     4621 gtccgtgtat tcccgcgctt tccgccagta cgtcgcgtcg ccggtcaatt cccaaccgtg
     4681 caggacaccg agaaggccgt atccttcgtg cctcgtcgtc caaaacctcc ggtccgtagt
     4741 gtatttcggg tcccagttgt tcaggcagaa tcgagccatg atgcggccgg cttcgagcat
     4801 gcgctcatcg ccggtcagca ggtagtgcag gtgcatcgcg cgggggtaga cgtactttag
     4861 atcgacgccc ttcaacgtga agatgcccgc gttcggaccg tccatctttg tatgcagacg
     4921 aacgaagtgc gcggcgtgat aggcggcctc cagatacttc ggatcgcctg tacggatgta
     4981 catcttgtac caacacgccg tgcggtcgaa cagccagtgg tcgtagactt ggctgtcttt
     5041 gtactgaagc gctcctggaa aactgtgctc cacaaagtcg tcgtaggcgg cgaacggccc
     5101 ggactccgaa gcaggaacct gcggcccggc gatccacgaa tcacagagcc acttcgcggg
     5161 caacacggcg agcacccgag ggccgtccag accgtcaggg tcaagaagtg tcgctgcgac
     5221 gggcacaaat cgcacgcagt ccttcctccg tttccttccg aacacgactt tgacgcgccg
     5281 cgtcctgcct ccggaaaagt cggctgtgaa ctggacgaga atggaacgga tcgagccgtc
     5341 ttttccgtcg atccgccacg gttcgagtgg gcgaacagag acggtgatct ctcggccgcc
     5401 cacgtcacgg aggctgacaa accgtgtatc gttgagatac ccaggcggca ggggaacgcc
     5461 gaaactaacc gtctcgcggc cgggcgcggc aaggcgcgcc tggagcgtga cctccacctc
     5521 tggagcaggc gccggaggcc gcggcctttc cctcgtctca accacacagg tgttggctac
     5581 ggccagcaaa acgaggacta agccgcaacc aaagggagcg gtcataagga accgcgatcg
     5641 agagaaagcg gaagctacat caagcagcac ccgacgggaa acctacagtt tcaccaagtc
     5701 ggagatcttc accgtctcgc cgccgcgctc gatgctgtgg taggcggcaa ttccgatgag
     5761 gctgccgtag gcgccggccc gcaggtctgc ccgcaggccc agcggatcgg ggtcgggctt
     5821 tccgcggaag accagatcct ggatcgacgt atcggagcca ccgtggccgc cctcacggcg
     5881 ctcctcgaag tcgcgaatca cctcggactt gccaaacgcc ctagtcagtc ggacttcacc
     5941 ctgcgagtag ccgccccctc cgaaatgatt ggcgtcgaga cgtcccttcg tgccattgaa
     6001 cgcgattgcc tgtccttcga acggctggta cgtgttcgcg gtgtacgtga gcatcacgtc
     6061 gttctcgtac tttacccgca ccgacatcgt gtcgtagatg ttgcagttct cacggaacag
     6121 gcaggcgtcg cgcaggtaac cgtcctcgga ttcgcagtcc gtgtacatct ccttcatgcg
     6181 ctcgctcttg gtgatgtccc aatagaactt gcactgctgc ttgaatgggc agacccggca
     6241 atgcgtgctc cggaacgtcc cgttgcgccc gtagaccttc agctcaccgt aggcggagac
     6301 ctccgtcggg gtggagttga gccaccagtt ggcctggtcg aagtggtgtg acgccttatg
     6361 gacgagcagg gtgccggagt tctccttcag ataatgccag cggcggaagt agtcggcgcc
     6421 gtgggaggtg tcaagatact cgtggaagtc caccgagacg atatcgccga cggccttctc
     6481 catcaggagc tgtttgacac gcttgtcctc ccggccgtgc cgggcgttga acgtgacggt
     6541 cactttgcgc ccggtacgct tcgccgcatc gaggatggcc tggcactgtt cctcgttggt
     6601 gcacatcggc ttctccgaga tggcgtcaca gccgagctcg agcgcgcgga caatgtagcg
     6661 ccagtgcgtg ccgtcgacgg tggtaatgat cacggcatcc ggccgggtct tcttgaccat
     6721 ttcgtcgaaa tcggtgaagg tgggcgcgct gatgcccatc atcccttgag cggccttcac
     6781 ccgcttggag ttgatgtcgc acaatccgac gaactcgaca acgtcgctgt aacccttgac
     6841 caccgggatg ccccaggtca acgtcccccg gctgcccgtg ccgacggttg caaccttcag
     6901 tttcttgccc tgggctcccg cgatgcccgt ggccaatggg ctggcggccg ccgctaacgc
     6961 ggtcccactc gtggcgagga aattccttct ggtcatcgaa tgatcgtttg actccggcat
     7021 gatgacgcct ccttaacgaa ctgtttgtac cacaattcag gatgccggca gtttggcggg
     7081 gatctccacg cgaatggccg ccttgaggat gcggcgagta ctgccctcag cgccgcccca
     7141 agaatgtccc taggaacgcg cccacggcaa tcccgatgaa acagactccg aggtcgcgcc
     7201 gccaacccag caccaccgga tttacggggc ggcgtctcac taggccaccg acacccatcc
     7261 agtgtgtgaa ccaaacggcg gcaacataag caaccaggaa tgctccgatc gctactgcca
     7321 gggcggttcc gaaggacgaa cccgcccggt gtgccgatac ggcggcagcc atggcggcag
     7381 caagggctgg aactgacggc ccgaagagcg catttcccga gagtggttcg ccgagtttcc
     7441 cggcaagaag gccgataagt actacaacaa gacccccgat gacgccgctc accaccgcgg
     7501 cggagtggct cgcaggagaa ctaccgatcc cggcatcgtc tttcatgggc aagaccccgt
     7561 cgatcccgtt ggctttcaat tctacatcca acctccaccg ctgacgctgg ttctttttct
     7621 taccgcgagc gactccttgc gcccgcgtct cacggactac cggaagtagc ggtcgaagaa
     7681 ctccttcatc gcggcgaagg cttccatcgt gaactcgtgc tggccgggct gccggtggac
     7741 gacaaggttt gtcttggccc cggcctgtgc gtacgccggc tccaccactt tcagaaactc
     7801 gtcgacgcct tctttcggca tgccgatgtc gtcaagcgga gcccagagca tcagcgggcg
     7861 cggcgcctgt gcggcggcaa agtcgccctg gtctccctta gtcagcatgt caggcaccca
     7921 ccagtagatc ccgtggtagc tccgtgcacc tttttcgaga aacgtctgga cggaa
//
LOCUS       DATIFQ010000460        15344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1112340, whole genome shotgun sequence.
ACCESSION   DATIFQ010000460 DATIFQ010000000
VERSION     DATIFQ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112340"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VLE22_23105"
     CDS             <1..997
                     /locus_tag="VLE22_23105"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB17357.1"
                     /translation="TASRSWCLTKCEATVMLWRAFAVAILSLGTLSAAGEEFSKTTYV
                     YKNAGGHEIKLDVMRPPDERVRPVILYIHGGALIFGSRGGINAEQLRRYIRAGFAVVS
                     IDYRLAPETKLPEILEDVRDAHQWVRANGRKLLHVDADRLAVVGHSAGGYLTLMTGIL
                     FKPRPKALVSYYGYGDIAGEWYSRPDPYYSSQPAVPEAEARAAVGTAPVSEPPQSSPR
                     PRFYLYCRQHGLWPKEVTGHDPDTEPRAYDPWCSVRNVTRQYPPTMLLHGDADTDVPY
                     RQSVMMAEELKRNGVEHELITIPGGPHGFDRAMDKPEISVIFDRAVQFIARRLKP"
     gene            1116..1592
                     /locus_tag="VLE22_23110"
     CDS             1116..1592
                     /locus_tag="VLE22_23110"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB17358.1"
                     /translation="MRLFLLVGVLLSVPVAGADKTFSVEAQVGAVKFVDSDTHFAIGG
                     AIGLPLSRRWSFKPEFLYLNESAEHDDLVFLPNLNFDFVGPGHRVRPFVTVAPGVLWY
                     REKYPHFRQTSTDFFLSGGGGAKVFVTDHVYVAPQVRTGVELHIGFTVAVGYEWGR"
     gene            complement(1618..2403)
                     /locus_tag="VLE22_23115"
     CDS             complement(1618..2403)
                     /locus_tag="VLE22_23115"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB17359.1"
                     /translation="MSIRLSPELSADEFVKWKQAGIDCVEIGPGRIDTAEAVAEIRKL
                     STQVQKWATSAGIEIWSVHVPFGKSLDISNPSEDQRQEVLRRLGGMLDAFAPLKAKKL
                     IVHPSSEPIPPEERQVRIDTCRRSLDTLARRARKMNVQIALEALPRTCMGNTSREILA
                     IVNGLDSVGICLDTNHVFQEKVDDFIRAVGPRIVTLHIADYDGKDEKHWLPGKGVNDW
                     TAIASALQETGYQGPFLFECQGTPEEKVAFWKQLRQAISAPGR"
     gene            complement(2614..4467)
                     /gene="asnB"
                     /locus_tag="VLE22_23120"
     CDS             complement(2614..4467)
                     /gene="asnB"
                     /locus_tag="VLE22_23120"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HSB17360.1"
                     /translation="MCGIVGIFAADLGVVEVREALDRMMRAVVHRGPDEAGSLIFGHH
                     RAGIGCRRLSILDLEHGSQPMPNEDGTVHVVLNGEIYNHCELRAELEQKGHTFRSRSD
                     TEVIAHLYEERGDDCLEALNGMFGLAILDSRSGRLLLARDRCGMKPLYFAQTSNGFLF
                     ASEIKSLLASGLVPAEPDLEAVDSFLTVGYVPVPRTGFRGVKKLEPGEALTVDAHGVT
                     PRVFWRVGFDNAKPPATEQEYAHELESRLTAAVKSHLVADVPVGAFISGGMDSSLVAA
                     LAVEHATYPLKTFSVVFPEDNSVDESRYSRELVQQLGTEHHEIEFRISEAPDLLPKMV
                     RHLEEPSTVGPGMATYKLASAAARHVKVVLSGEGSDEIFAGYAWLGSEAYYRIRPLVP
                     RIFMRKLAEHSRSLRVRRVLRTLAAPDEVSADIEWFRIMTPNEKARALRLTAPSSDLD
                     PIRPHPDTLASSADILQRRLALDFTRRLANGILLMGDKMTMAHSLETRMPFLDRGVVD
                     FGLALPSRMKWRGKRGKYILSLLANRLPPEIAHRRKRGFQYPIRSMQRGPFADFARAY
                     LLDSAKPGGLIDRHFLESALAQWQGEGPAGLRLITALLTLRAWCNEFFPGL"
     gene            complement(4467..5855)
                     /locus_tag="VLE22_23125"
     CDS             complement(4467..5855)
                     /locus_tag="VLE22_23125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17361.1"
                     /translation="MQPTASDFRGLFGDRRIVFHAILGSYCAGLAPLCPIEVVACADW
                     GREFAHFGDGIRFLSRERKTSRRLRTHDYDIEAILESVRDELLRLLFGDPSGRWVMVC
                     PYASPALDAFGTATGYPVFTHPVALAEWLNDKSNFFRGLDELGLPRLPGRWVRLTEAR
                     YSDLASELGSRFIAQEGRGSGGSGTVWISSLPDFVTAGERFGDVLVWAAPDAGPLSLN
                     INAIALSRSAVAGYPSVQLAGIPELGAGPGAYAGNDYTAIATLPSDVVRGVREQTERI
                     GEWLGSLGYRGLYGLDFVVDMDTSRCFAVDLNPRWQGSTALATQGENFEGRIPLAAAE
                     LARRIGLLGEAEVAYLREEFFKPVRGSQMILRQNRAEWLEIAAGVPAGVYSVRDGLTH
                     LRPGATLGDCGPGEVLVTGGVPERGTVVESGAYMARICSQQAALDTERMELVPWARLA
                     VSRLYGVLAPER"
     gene            5915..7249
                     /locus_tag="VLE22_23130"
     CDS             5915..7249
                     /locus_tag="VLE22_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17362.1"
                     /translation="MPVVWSEFVRGVYGLVAGRNASRDQIEAFQTRALRSVVRHAYER
                     VPYYRRLFVRAGLKPEDVRGLDDLPRIPITSRQDLQELPASDLIVSGVDASSLLTHRT
                     SGSSGAPLTIRRERFEEYLLLAYRWRGHIAQGRHLTDREASIGYIHDAGDGRSHPKEP
                     LHERLSQLWKDWIDCLLPSKKILERLRVTQPEALHGTPSLLAWLAEEMTDADRRLIRP
                     RIVSCSQETLSDSVRQRISAAFGGKVFNNYGAHEFVGVASECPHRHGYHVSDWTLILE
                     ALRDGHPAEPGEQGEVVGTALHSYAMPFIRYRLGDLVVRGPAPCPCGAPFSTLLSIEG
                     RTLDMIRLPDGRSIHPYAVFGPLVESVPWVRRFQVIQERADSFRVKIVTRTETGPDAL
                     DRMAGAIREALGPRVQVETELTDQLPTGRRGKFHPFVSLERLNAWRQAGQSL"
     gene            complement(7241..8950)
                     /locus_tag="VLE22_23135"
     CDS             complement(7241..8950)
                     /locus_tag="VLE22_23135"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HSB17363.1"
                     /translation="MSRLFYLRDSVMLVTMQKRWGIAMFLAAGALFGDEVPTRQQVLD
                     AMRKAVGFYVDQVSKEGGYHFVYAEDLSYGRSEQGDGLMQIETQREGTPRVGMAFLEA
                     YDATGDRFFLEAARKAAQALVKGQLCSGGWDYIVEFDPEKRPKYPYRADRPCDASTPQ
                     PPPSTLDDNVTQAAVRLLMRVDRALGFQDREIHDAAQFALESLLKAQYPNGAWPQRYS
                     KFPDPSQFPVKKASYPEDWPRQWPGEVYKTHYTFNDNSIQDAIDMMLEAARVYGDRRF
                     VESARRGGDFILSAQMPEPQPAWAQQYDAEMHPAWARIFEPPSVTGGESQSVMRALLL
                     LYRETGDRRYLDAVPKALNYLERSVLPPASGPVEARRRFKPGEPVLARFYELKTNRPL
                     YITKGSRVSARGMGSKLLDGYELSYADESVITHYNVLTSGAGLAEIRREYERLAGAAP
                     STLRRPDKLHGLSPWEQRDTGPLAERPAVERVRIIIGAMDPRGAWLEDGVIGKADRIV
                     SVYAARDMVLTVNGKATPIRENDQIELYQGAKPPRTRIIRSTTFAENLETLAAWVSKE
                     GSE"
     gene            8949..10826
                     /locus_tag="VLE22_23140"
     CDS             8949..10826
                     /locus_tag="VLE22_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17364.1"
                     /translation="MKYLIFVAFLPAVLLSPVLAGESATIVVKTPMAPPEWALLERDL
                     LRFNSIGCERFAERYVDERGFLLHTPRWGTLDGPDDAIETFFNWTLLHALGGSDSVLQ
                     LYKKAQEGHWKQYNELRTTLTELAENGAYYKEFITQSDWFHTGEGMRAFLLLGLSDPN
                     NGLYRERMKRFAGLYMNEDPEAPNYDPVHKVIRSIWNGSKGPMLRKATVYDWVGDPVP
                     GSFHLLHNPAGYSKLLDMKTYYPKMLAHCTEYLDSVGDNSLNLAATILGLDAYMLTGE
                     AKYKAWVLEYVNAWKERAAACGGNIPTNVGLDGKPGGEYGGQWWKGTYGWNFTIFDGE
                     LEQIAHRNYVHAGSWPGFGNALLLTGDQGYVDVLRRQMDNFYAQKKVEGGKVLLPQMY
                     GDPRGYQHDGPPSWYHYTPELHSDRLTEIYLWSMDRRDLERVPKAGWIGFLEGDEPQF
                     PEKELRADLEQVRQRLERMRGDGTSADTRLADYLLPFNPAATDTLVNLMLGGYFARGR
                     IWVLHSRFRYFDPAKRRAGVPEDVAALVEKLSADSATLTLVNVNPVDVREVVVQAGGY
                     GEHQFDEVTAEGRTAPVAAPVLTVRLEPGCGARLVFRMSRYKNQPTFAFPWDRGWYGR
                     P"
     gene            complement(10823..12244)
                     /locus_tag="VLE22_23145"
     CDS             complement(10823..12244)
                     /locus_tag="VLE22_23145"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HSB17365.1"
                     /translation="MAQSNPSIKKKIVWTVIITALATIGVSALLINILERKQEARNPF
                     FRVVELADDTIDPAVWGKNFPMQYDLYKRTVDQERTRYGGSEALPRTPTQADPRSVVA
                     QSKLEEDPRLVTMWKGYAFSVDFREERGHAYMLIDQRMTRRVQEFKQPGTCLQCHAST
                     YVAMQKLGGGDLFKGFEAMNKMPYAEATKLVEHPVSCIDCHDPKTMRLRVTRPAFMEG
                     IRALKASQGVADYDVNTMASAQEMRSFVCGQCHVEYYFKGDEKRLVFPWSKGLKAENM
                     LAYYDEVGHKDWVHAETGAPALKAQHPEFEMWNQGVHARSGVACADCHMPYKREGGLK
                     ISDHHVRSPLLNVNRACQGCHHFSEQEMKSRVEEIQGRFHMARNVAMDALMELISDLK
                     KAKESGASEAALKPAQEFHRKATFFLDLVEADNSSGFHAPGEELRILTQVVDFCRKGQ
                     LALRGAQPTQKASHATSTGPVGD"
     gene            complement(12275..12730)
                     /gene="nrfH"
                     /locus_tag="VLE22_23150"
     CDS             complement(12275..12730)
                     /gene="nrfH"
                     /locus_tag="VLE22_23150"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HSB17366.1"
                     /translation="MSEHGYITPLLLGVPLGILLGLGFYTFGYARGAAYMTDDPDACT
                     NCHVMRGQYESWMKSSHRKVAVCNDCHTPAGLAPKYFTKALNGFNHSMAFTTGRFPDE
                     IRITARNHRVAEASCEKCHADIVSGINGVRHDGEKVSCSTCHRAVGHAY"
     gene            12817..14277
                     /locus_tag="VLE22_23155"
     CDS             12817..14277
                     /locus_tag="VLE22_23155"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HSB17367.1"
                     /translation="MQLPRLISARVLLPLLAVVCLTAQTQPVKSAPLTVQGSFRTRIE
                     AWDWFEGSADNSYAFSGNLLRLTLGQQRKQIDWQLELAAPFLLGLPENAIAPAPQGQF
                     GLGASYYAANDAKRNAGMVFAKQAFVRFKGLFGDPRQSLRLGRFEFIDGSEVAPRNPT
                     LAALKRDRIAHRLLGNFGFSHVGRSYDGIQYGNNRPKVNYTILAAMPTRGVFQVDGWG
                     NLKTGLVYAAATGQVAGKKSSGEWRVLGLYYHDWRRVLKTDNRPLATRRSDTGNIRIG
                     TLGGHYLHAWETPSGTVDILLWGVLQVGRWGVLDHRSGALSTEAGWQPKAVPALKPWL
                     RAGFFHGHGDGDPNDTTHGTFFQVLPTPRIYARFPFFNLMNSEDLFGSVTLRPHKTVS
                     IKTEVHGLRLASRNDLWLLGGGAFQPWTFGYVGRPSNGKRGLANLYDASIDWLVSPVA
                     TLSAYYGYATGGAVIEKIYPNGKNGSLGYLELSYRF"
     gene            complement(14274..14864)
                     /locus_tag="VLE22_23160"
     CDS             complement(14274..14864)
                     /locus_tag="VLE22_23160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17368.1"
                     /translation="MKHVVGAAMFVAASACMFAQQRGAVNPVRTFPSESSLPLLSPIP
                     PIGNTPRPQNIVPGFQRPRWQPYGYPSMFPWYGGYGGYVGGAQPSQNVVVVQTPPPAP
                     AEVYRPEPPPPPAKPEVRDYRWEEEIRSTASEFVIALRDGTEEAAEAVWVHGGTLHYA
                     TPRAIDRAVPLRLVDRERTESLNRQRGLRLRLPPGD"
     gene            14989..15237
                     /locus_tag="VLE22_23165"
     CDS             14989..15237
                     /locus_tag="VLE22_23165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17369.1"
                     /translation="MNRELPIETLADEIYSVVGQQQGKRYLRAGDLTQMMMERFGEEC
                     SKDNCRRAIHQLIDSGRCVYSYFGASYITLPHKAKKQP"
BASE COUNT         2873 a         4756 c         4717 g         2998 t
ORIGIN      
        1 taccgcgagc cgttcgtggt gcctgacaaa gtgtgaggcg acggtgatgc tctggcgtgc
       61 gtttgcggtg gccattctga gtctcggcac gctcagcgct gccggcgagg agttctccaa
      121 gacaacgtac gtgtataaga acgccggcgg gcacgagatc aaactcgatg tcatgcgccc
      181 gccggatgag cgcgtgcgcc cggtgatcct ctatatccac ggcggcgcac tcatcttcgg
      241 aagccgcggc ggcatcaatg cggagcagtt gcgccggtat atccgggcgg gcttcgcggt
      301 tgtgtcgatc gactaccggc ttgctccgga gaccaagctt ccggaaatcc tcgaggacgt
      361 ccgcgacgcg caccaatggg tgcgggcgaa cggccggaag ctgcttcacg tcgatgcgga
      421 ccggttggcc gtggtcgggc actcggcagg cggctacctg acgctgatga cgggcatcct
      481 attcaagccc cggccgaagg cgctcgtgtc gtattacggt tacggcgata tcgcgggcga
      541 gtggtacagc cggcccgacc cgtactactc gagccagcct gcggtgccgg aggccgaggc
      601 ccgcgcagcg gtcggtacgg cgccggtttc ggagccgcca cagtcaagcc cgcggccgcg
      661 attctacctg tattgccggc agcacggtct gtggccgaag gaagttacgg gccacgatcc
      721 ggacaccgag ccgagagcat atgacccgtg gtgttccgtg cgcaacgtga cgcgccaata
      781 tccgcccacg atgctgctgc acggcgatgc ggacacggac gtgccgtatc gccagtcggt
      841 gatgatggcg gaggaactga aacggaacgg cgtcgagcac gaactgatca cgattccagg
      901 cggtcctcac ggattcgacc gggcgatgga caagccggag atctcggtga ttttcgaccg
      961 ggccgttcaa ttcattgcgc gtaggctgaa accctagcac cttcttttcc ctggcacagt
     1021 gtcgggcagg gctgacagtc tgcacataaa ccttccgtca acgccatgta tttccaacgc
     1081 gatgcgttgg cattccgctt gctcccttgt gccgcatgcg attgtttctt ttggtgggtg
     1141 tcttgctgtc tgtccccgtc gcgggtgcgg acaagacgtt ttcagtagag gcccaggtgg
     1201 gcgccgtcaa attcgtggac agcgacactc actttgccat cggcggggcg atcggcctgc
     1261 ctctgagccg caggtggtcg ttcaagccgg agttcttata cctcaatgag agcgcggagc
     1321 acgacgacct ggtgttcctt ccgaacctca acttcgactt tgtcggacca ggtcatcgtg
     1381 tgcggccgtt cgtcacggtg gcgccgggtg tcctgtggta ccgggagaag tatccgcact
     1441 tccggcagac atcgacggac ttcttcttga gcggcggtgg tggggcgaag gtcttcgtga
     1501 ccgatcacgt gtacgtcgca ccgcaggttc gcacaggcgt cgagcttcat attggtttca
     1561 ccgtcgcggt cgggtacgag tggggccgct gacgcgcgtg tatccctgcc gtcgtattca
     1621 cctgcctggc gccgatatcg cctgcctcaa ctgtttccag aaggcgacct tttcttccgg
     1681 cgtgccctgg cactcgaaca ggaagggtcc ctgatagccg gtctcctgca aagccgaagc
     1741 gatcgccgtc caatcattca cgcctttgcc gggcagccaa tgcttttcgt ccttgccgtc
     1801 gtagtcggca atgtggagag tgacaatacg cgggccaaca gctctgatga aatcgtcgac
     1861 tttctcctgg aagacatggt tcgtatccag acaaatgccc accgaatcga ggccattgac
     1921 aatcgccaga atctcccggc tcgtgtttcc catacacgta cgaggcaggg cctcgagcgc
     1981 gatctgcaca ttcatcttgc gggccctccg cgcgagtgtg tcgaggcttc ggcggcacgt
     2041 gtcgatgcgg acctgacgct cctcgggagg gatcggctcg gaacttggat ggacgatgag
     2101 tttcttcgcc ttgagcggcg caaacgcatc gagcatgccg ccgaggcgcc gcagaacctc
     2161 ctggcgctgg tcttcggacg gattcgagat gtcgaggctc ttgccgaacg ggacgtgaac
     2221 ggaccagatt tcgatgcccg ccgacgtcgc ccatttctgc acttgtgtgg acagttttcg
     2281 aatctcggcc accgcctcgg ccgtatcgat gcggcccggt ccgatttcca cgcaatcgat
     2341 acccgcctgc ttccacttga cgaactcgtc cgcagagagc tccggagaaa gccggatgga
     2401 cataccggtt ttccaggttc gagcatcgga cccggcggcg agaagactgc cggccaatag
     2461 gagaagtgag aaggggcgga tgagtttcgg catttccctg gtatacatca acaactgcgg
     2521 ggtccgcgcg gggccagagg acccggcgtt gatgcccccg gccggaacct gtgggtgcgg
     2581 tacaggttca cgttctgaaa acggctgctg cggtcaaagc cccgggaaga actcgttgca
     2641 ccacgcccgc aaagtcagta gcgcagtgat gagccgcagt ccggcagggc cctcgccttg
     2701 ccattgcgcg agggccgatt ccaggaaatg gcgatcgatc agtccgccag gctttgccga
     2761 atcgagcagg tacgcgcggg cgaagtcagc gaacggtccc cgctgcatgg aacggatagg
     2821 gtactggaaa ccgcgtttcc tgcggtgcgc aatttcgggg ggcagtctgt tggcgagcag
     2881 cgagaggatg tacttgccac gcttcccccg ccacttcatc cgcgatggaa gcgcaagacc
     2941 gaagtcaacc acgcctcgat cgagaaacgg catgcgcgtt tcaagcgaat gggccatggt
     3001 catcttgtct cccatcagca gaatgccgtt cgccagccgc cgggtgaagt cgagagccag
     3061 ccgtcgttgg agaatgtcgg cggaggatgc cagcgtgtcc gggtgtggcc ggatagggtc
     3121 gagatcgctc gacggagccg tcagccggag tgcgcgggcc ttctcgttcg gagtcatgat
     3181 gcgaaaccat tcgatgtcag ctgacacttc atccggtgcg gcaagcgtgc ggagcacgcg
     3241 ccggacccgc aagctccggc tgtgctccgc caatttccgc atgaaaattc gcgggacgag
     3301 cggccggatc cggtagtacg cttcggaccc cagccacgcg tagccggcga agatctcgtc
     3361 cgaaccttct ccgctcagaa cgaccttgac atgccgcgca gcggccgacg ccagcttgta
     3421 cgttgccatc cccggcccga cggtgctcgg ttcctccagg tggcgtacca tcttcgggag
     3481 caggtccggc gcctccgaga tgcggaactc gatctcatga tgttccgtgc cgagttgctg
     3541 aacgagttcc cgagagtagc gactctcgtc aacagaatta tcctcgggaa aaacgacgga
     3601 gaaggtctta agagggtaag tcgcatgttc cacagcaagg gcagcgacga gagatgagtc
     3661 cattcctccg ctgatgaacg cgccgacggg gacatccgcc accaggtgcg atttcaccgc
     3721 ggcggtgaga cgggattcga gttcgtgcgc gtattcctgc tcggtggccg gtggcttcgc
     3781 gttgtcaaag cctacacgcc agaaaacgcg gggggtgacg ccgtgggcgt caacggtcag
     3841 cgcctcgccc ggttcgagtt ttttcacgcc gcgaaagccg gtccgaggaa cgggaacgta
     3901 ccccaccgtg aggaaggaat cgacggcctc caggtctggc tctgccggga cgagtcctga
     3961 agcgaggagg ctcttgattt cggaggcaaa caggaacccg ttcgaagtct gagcgaagta
     4021 cagcggcttc ataccgcaac ggtctcgagc aagcagcaac cggcccgacc ggctatcgag
     4081 aatcgcaagc ccgaacattc cgttcaatgc ttccaggcaa tcgtcgcccc gctcctcgta
     4141 cagatgtgcg atcacttccg tgtcggagcg gcttcgaaag gtgtgtccct tttgttcgag
     4201 ttcggcgcgc agttcgcaat ggttgtaaat ctctccattg agcacgacat gcactgtccc
     4261 gtcttcattt ggcatcggct ggctgccgtg ctcgaggtcg aggatgctca gtcggcgaca
     4321 gccaattccg gcacggtggt ggccgaaaat cagactgccg gcctcgtccg ggccccgatg
     4381 cacgaccgcc cgcatcatgc ggtcaagcgc ttcacggact tcgacgacgc cgagatccgc
     4441 cgcgaaaata cctacgattc cgcacattac cgttcgggcg ccagaacgcc gtaaagccgg
     4501 ctcactgcga gacgcgccca gggaacgagt tccatccgtt ccgtgtccag tgccgcctgc
     4561 tggctgcaaa tccgcgccat gtacgcgccg ctctccacca ccgttccccg ttccggcact
     4621 ccgccggtca ccaaaacctc tccgggaccg cagtcgccga gcgttgcgcc gggtctcagg
     4681 tgtgtcaaac cgtcccgaac gctgtagaca ccggccggca cgccggccgc gatctccagc
     4741 cactctgctc gattctgtcg caagatcatc tgagagccgc gtacgggttt gaagaattct
     4801 tcccgaagat aggcgacttc cgcttccccg agcagcccga ttctccgcgc cagttcggcc
     4861 gcggcgaggg ggattcttcc ttcgaaattt tcgccttgcg tggcgagggc ggtggagcct
     4921 tgccaccgcg gattcagatc gaccgcaaag caccgggatg tatccatgtc cacgacgaaa
     4981 tccagcccgt acaaccctcg gtaaccgagc gatccgagcc attcgccgat tctttcggtt
     5041 tgctccctga cgcctctgac cacgtcggac gggagtgtgg cgatggcggt gtagtcgttt
     5101 cccgcgtacg ctcctggtcc cgccccaagt tccgggatgc ctgccagttg cacactcgga
     5161 taaccggcaa cggccgagcg tgagagtgcg atcgcgttga tgttcagtga cagcgggccg
     5221 gcgtctggcg ccgcccaaac gagaacgtct ccgaaccgct cgccggcggt gacgaagtcg
     5281 gggagggagc tgatccagac ggttcccgag ccgccgctcc cgcggccttc ttgagcaatg
     5341 aaacgcgaac cgagttccga tgccaagtcc gagtaacgcg cttccgtcag gcggacccaa
     5401 cggcccggta gccgcgggag cccgagttcg tcgaggccac ggaagaagtt cgacttgtcg
     5461 ttgagccatt cggcaagcgc aactgggtgg gtgaagaccg gataacccgt cgcggtcccg
     5521 aaggcgtcga gcgccggact ggcatagggg cacaccatga cccagcgtcc ggacggatca
     5581 ccgaaaagca accgaagcaa ttcgtcgcgc acgctctcta aaatcgcctc gatgtcgtag
     5641 tcatgggtgc ggagccgcct gcttgttttg cgctcccttg aaagaaatcg gataccatcg
     5701 ccgaaatgcg cgaattccct tccccagtcg gcgcatgcga caacctcgat tggacacagc
     5761 ggcgcaagcc ccgcgcagta cgagccgagg atcgcatgga acacgattcg ccgatctcca
     5821 aaaagcccgc ggaaatcgga cgccgtcggc tgcattcgcc gattctacca gccggacggg
     5881 attccgcctg tcggaccaaa ggtagaattc gaagatgccg gttgtttgga gcgagttcgt
     5941 acgtggcgtg tatggccttg ttgcgggccg gaacgccagc cgtgaccaga tagaggcctt
     6001 ccagaccagg gcgctccgct ctgtcgttcg gcacgcgtat gagcgggttc cttactacag
     6061 gcgtctattc gttcgagcgg ggctgaagcc cgaggatgtg cgtggtctcg acgatctgcc
     6121 gcggattccg atcacttcgc gccaagacct tcaggaactg ccggctagcg acctcatcgt
     6181 ctcgggtgtc gatgccagct cactgctaac ccaccgtacc agcggatcga gcggcgctcc
     6241 tttgacgatc cgccgcgagc gattcgagga gtacttgctg ctcgcctacc gctggagagg
     6301 gcacatcgcc cagggaaggc acctgacgga ccgcgaggct tcgatcggtt acattcacga
     6361 cgcgggggac ggccgaagcc atccgaagga gccgcttcac gaaagacttt cccagctctg
     6421 gaaggactgg atcgactgcc ttctgccctc gaagaagatt ctcgagcgtc ttcgagtgac
     6481 tcagcccgag gcgctgcacg gaacacccag cctcctcgcc tggctggccg aagagatgac
     6541 cgacgccgat cgacgtctga tacgcccgcg gatcgttagt tgcagccagg agactctcag
     6601 tgattcggtc cggcagcgga tctccgccgc tttcggcggc aaggtcttta acaactacgg
     6661 cgcgcacgaa tttgtcggag tagcttcgga gtgcccgcac cgccacgggt atcacgtctc
     6721 cgactggaca ctgattctcg aggctctgcg tgacggccac cccgccgaac cgggcgagca
     6781 gggggaagtg gtaggcaccg cgctgcactc gtatgcgatg ccgttcatcc gttaccgcct
     6841 gggtgacttg gtagttcgtg ggccggcgcc ctgtccctgc ggtgcgccgt tttcgaccct
     6901 gctctctatt gagggccgga cgctggacat gattcgcctg ccggatggcc gttcgattca
     6961 tccctacgcg gtattcggcc cgctcgtcga gtcggtcccg tgggtccggc gtttccaggt
     7021 gatccaggag agagcggatt ccttccgggt caagattgtg acccgcacgg aaaccgggcc
     7081 tgacgcgctc gaccggatgg ctggggcgat ccgagaagct cttggaccga gggttcaggt
     7141 tgagaccgaa ctgactgatc agctcccaac cggccggaga ggaaaattcc accccttcgt
     7201 ttcgctcgaa cgactcaacg cttggaggca ggctggccag tcactctgag ccttccttgg
     7261 acacccatgc cgccagggtt tctaggtttt cggcgaaggt ggtcgagcgg atgatacgcg
     7321 tgcggggcgg cttggccccc tggtacagtt cgatctgatc gttttcgcgg atcggcgtgg
     7381 ccttaccgtt gacggtcaac accatgtcac gtgcagcgta gaccgagaca atccggtcgg
     7441 ctttgccgat cacgccgtcc tccagccaag cgcctcgtgg gtccattgct ccaatgatta
     7501 tccgcacgcg ctccaccgca ggacgttcag ccaaagggcc ggtgtcacgc tgctcccacg
     7561 gggaaagtcc gtgcagtttg tccggccgcc ggagtgtgga cggagccgcc cccgccagcc
     7621 gttcgtattc ccgccgaatc tccgccaggc ccgccccgct cgtcagtacg ttgtagtgtg
     7681 tgatgaccga ttcatctgcg taactcagct cgtagccgtc gagaagcttt gaacccatac
     7741 ccctcgccga caccctcgat cccttcgtga tgtacagcgg gcggttcgtc ttcagttcat
     7801 aaaaccgtgc cagcactggc tcgcccggtt taaagcggcg gcgcgcttcg accggcccgc
     7861 tagccggagg aagaaccgaa cgctccaggt agttgagggc cttcggcacg gcgtcgagat
     7921 acctccggtc gcccgtctcg cggtaaagca gcagaagggc tctcatcacc gattgagact
     7981 cgccacccgt caccgaaggc ggctcgaaaa tgcgcgccca cgccggatgc atttcggcgt
     8041 cgtactgttg agcccatgcg ggctgcggct ccggcatttg ggccgacagg atgaagtctc
     8101 ctccgcggcg cgccgattca acgaagcggc ggtccccgta gactcgcgcc gcttcgagca
     8161 tcatgtcgat ggcatcctga atcgaattgt cgttgaacgt gtagtgcgtc ttatagactt
     8221 cgcccggcca ttgcctcggc caatcttccg ggtagctcgc ctttttcacc ggaaactgtg
     8281 aagggtcggg aaacttggaa taccgctgcg gccaggcgcc gttcgggtac tgcgctttga
     8341 gcaggctttc gagggcgaac tgcgcggcgt cgtggatctc acggtcctgg aacccgagcg
     8401 cgcgatccac acgcatcagc agccgcacag cagcctgggt cacgttgtcg tcgagggtcg
     8461 agggaggagg ctggggcgtg gacgcgtcgc acggtctgtc ggcgcggtag gggtatttcg
     8521 ggcgcttctc cgggtcgaac tcgacgatat agtcccaacc tccggagcac aactgtcctt
     8581 tgacgagcgc ctgtgccgcc ttgcgcgccg cttccagaaa aaatcgatct ccggtggcat
     8641 cgtacgcctc gagaaacgcc atgccgacgc gcggcgtccc ctcgcgctgc gtctcgatct
     8701 gcatcagccc atcgccctgc tcggatcggc cgtagctgag atcctcggcg tagacgaagt
     8761 ggtagccgcc ctctttggat acttggtcca cataaaaacc cacagccttt cgcatggcat
     8821 cgagtacttg ctgccgggtg ggcacctcat ccccgaacag agcgccggct gcaaggaaca
     8881 tagcgatccc ccatcgtttt tgcatggtga caagcataac cgagtcgcgg agatagaata
     8941 gacgcgacat gaagtatcta atctttgtcg cctttctgcc tgccgtactt ctctcacccg
     9001 tcctcgctgg agagagcgcc accatcgtcg tgaagacccc aatggcccct cccgagtggg
     9061 cgttgctcga gcgtgatctc cttcgtttca actcgattgg ctgtgagcgc ttcgccgagc
     9121 ggtacgtcga tgagcgcggc ttcctgctcc acacgccaag gtggggtacg ctcgacggtc
     9181 cggatgacgc tatcgagacg ttcttcaact ggaccctgtt gcacgcgcta ggcggatcag
     9241 actcagtgct tcagctctat aagaaagccc aggaaggcca ctggaagcag tacaacgaat
     9301 tgcgtaccac gctgaccgag cttgccgaga acggcgccta ctacaaggag ttcatcacgc
     9361 agtctgactg gtttcatacg ggggagggga tgcgtgcgtt tctgctcctc ggcctctcag
     9421 atcccaacaa cggcctgtac cgggaacgga tgaaacgctt cgccggtctc tacatgaatg
     9481 aagatcccga ggcgcctaac tacgatccgg ttcacaaggt gattcgaagc atctggaacg
     9541 gcagcaaagg tccgatgctg cggaaggcga ccgtctacga ctgggtcggc gatccggtac
     9601 cgggcagttt ccatctactc cacaatccgg ccggttactc aaagctgctc gacatgaaga
     9661 cgtactaccc gaagatgctc gcgcattgca cagagtatct cgacagcgtc ggcgacaatt
     9721 ccctcaacct ggccgccacg atactcggtc tcgacgccta catgcttacc ggcgaagcga
     9781 aatataaggc gtgggtgctc gagtacgtca atgcctggaa ggagcgtgcg gcggcctgcg
     9841 gcggcaacat cccgaccaat gtcgggctgg acggcaagcc cggcggcgag tatggcggac
     9901 agtggtggaa gggaacgtac ggctggaatt tcaccatttt cgacggcgaa ctcgaacaga
     9961 tcgcgcaccg gaactacgtc cacgcgggct cctggcctgg attcggcaat gcactgctgc
    10021 tgaccggcga ccagggctat gtggatgtgt tgcgccggca gatggacaac ttctacgccc
    10081 aaaagaaggt tgaaggcgga aaggtgctgc tgccgcagat gtacggcgac ccccgcggtt
    10141 accagcacga cggcccaccc tcctggtacc actacacacc ggaactgcat tccgaccggc
    10201 tcaccgagat ctacctttgg tcgatggacc gccgggacct ggaacgggtc ccgaaggctg
    10261 ggtggatcgg cttcctcgaa ggcgacgagc cccagttccc ggagaaagag ctgcgagccg
    10321 atctggagca agtgcgccag cggctggaac ggatgcgtgg agacgggacc tcagcggata
    10381 cccggctcgc cgactatctg cttcccttta acccggccgc caccgacacg ctggtgaacc
    10441 tgatgctcgg cggctacttc gcgcgagggc ggatctgggt gcttcacagc cgcttccggt
    10501 acttcgaccc ggcgaagcgc cgcgccggag tgccggagga tgtcgcagct ttggtcgaaa
    10561 aacttagcgc ggattcggcc acccttacgc tggtcaacgt gaatccggtg gatgttcgtg
    10621 aagtcgtcgt gcaggccggc ggctacggcg agcaccagtt cgacgaagtc accgccgagg
    10681 gccggactgc acccgtagcg gcgcctgttc ttacggtccg tttggagccc ggttgtggcg
    10741 cgcgcctcgt cttcaggatg agccgctaca agaaccagcc gaccttcgcg tttccctggg
    10801 accgtggctg gtacggccgg ccttagtcgc cgaccggtcc cgttgaagtt gcgtgcgagg
    10861 ccttctgtgt gggctgtgcg ccgcgcagtg cgagttggcc cttgcggcag aaatcgacca
    10921 cttgtgtcag gatccgaagt tcttcacccg gggcgtggaa gccactcgaa ttgtccgcct
    10981 ccactagatc gagaaagaaa gtggctttgc ggtgaaactc ctgcgccggc ttcagcgcag
    11041 cctcgctcgc accggattcc ttcgccttct tgaggtcgga gatcagttcc atcagcgcat
    11101 ccatcgctac attgcgcgcc atgtggaaac ggccttggat ttcttcgacg cgcgacttca
    11161 tctcctgttc ggagaagtga tggcagccct ggcaggcgcg gttgacgttg agcagcgggc
    11221 tgcggacgtg atggtcgctg atcttcaatc cgccctcacg cttgtacggc atgtggcagt
    11281 ctgcacaggc cacaccggag cgtgcgtgga cgccctggtt ccacatttcg aactccggat
    11341 gctgcgcctt gagcgccggg gcgccagttt cggcatggac ccagtccttg tgcccgacct
    11401 cgtcgtagta cgctagcatg ttctccgctt tcaggccctt cgaccatggg aacaccaggc
    11461 gtttctcatc acccttgaaa tagtactcca cgtgacattg gccgcagacg aagcttcgca
    11521 tctcctgcgc ggaagccatt gtgttcacat cgtagtccgc tacgccctgc gaggctttca
    11581 acgctcggat cccctccatg aaggcagggc gagtgacgcg aagcctcatc gtcttcggat
    11641 cgtggcaatc gatgcaggac acgggatgtt ccaccaactt cgtggcctcg gcatacggca
    11701 tcttgttcat ggcttcaaac cccttgaaca gatcgccgcc gcccagtttc tgcattgcca
    11761 catacgttga ggcgtgacac tggaggcagg ttccaggctg cttgaactcc tgaacgcgcc
    11821 gtgtcatccg ctggtcgatg agcatgtagg cgtggccacg ctcctcccgg aagtccaccg
    11881 agaacgcata ccccttccac atggtgacga gcctgggatc ctcctccagt ttgctctggg
    11941 cgacaaccga gcgcgggtcg gcctgcgttg gggtgcgagg caaggcttcg gagccgccgt
    12001 agcgcgtgcg ctcctggtcg accgtccgtt tgtagaggtc gtactgcatc gggaaattct
    12061 tgccccatac ggcgggatct attgtgtcat cagctagctc gacgactcgg aagaaggggt
    12121 ttcgcgcctc ctgtttccgc tcgaggatgt tgatcagcaa agcgctgacc ccgatggtcg
    12181 cgagcgccgt aatgatgacc gtccagacga tcttcttttt gatcgaggga ttgctctgtg
    12241 ccatagttct gtctttatct tgaagacatg tggatcaata ggcgtgcccg acggcccggt
    12301 ggcacgtcga gcaggagacc ttctcgccgt cgtgtcgaac gccgttgatg ccgctcacga
    12361 tgtcggcgtg gcacttctcg caagatgctt ccgccactcg gtggttgcgg gccgtgatcc
    12421 ggatctcatc ggggaaacgg ccagtggtaa acgccatgga atggttgaat ccgttcaacg
    12481 ccttcgtgaa gtacttcggc gctaaacccg ccggcgtgtg gcagtcgttg cacaccgcca
    12541 ccttgcgatg actggatttc atccagctct cgtactggcc gcgcatgacg tgacagttcg
    12601 tacaggcgtc cgggtcatcc gtcatgtagg cggcgccgcg tgcgtagccg aacgtgtaga
    12661 agcccaaccc aaggagaatg ccaagcggca caccaagcag aagcggcgta atgtacccgt
    12721 gctcgctcat gacctgttcc agtatattca tagccgaagc tattttttgt gatcagagtc
    12781 accagaatca aacatcaggt cttttactct tgttctgtgc aactacctcg cctgatctcc
    12841 gcccgtgttc tcctgccact tcttgccgtc gtctgcctga ccgcgcagac ccagcccgtg
    12901 aagtccgcgc cactgaccgt ccagggaagt ttccggaccc gcatcgaagc ctgggactgg
    12961 ttcgagggaa gcgccgacaa cagctacgcg ttctccggaa accttttgcg gctgaccctc
    13021 ggccaacagc gcaagcagat cgactggcaa ctggagctgg ccgcgccgtt cctgctcggg
    13081 ttgccggaga acgccatcgc gccggcgccg cagggccagt tcgggctggg cgcaagttat
    13141 tacgccgcta acgatgcgaa gcgcaatgcg ggtatggttt tcgcgaagca ggccttcgtc
    13201 cggttcaagg gcctcttcgg cgacccgcgc cagagcctgc ggctgggccg cttcgagttc
    13261 attgacggct cggaggtagc gccgagaaac ccaacgctgg ctgcgctgaa gcgggaccgc
    13321 attgctcacc gcctgctcgg caacttcgga ttcagtcatg tgggtcggag ctacgatggc
    13381 atccaatacg gcaacaaccg gccgaaggtc aactacacga ttctggcggc gatgccgacc
    13441 cggggcgtct tccaggtgga tggctggggc aacctgaaga ccggcctggt ctatgccgcc
    13501 gccacgggcc aggtagcggg caagaagagc agcggtgaat ggcgggtgct gggcctctac
    13561 taccacgact ggcggagggt gttgaagacg gacaaccggc cgctggcgac acgccggagt
    13621 gacacgggca atatccgcat cggcaccttg ggcggacact atttgcacgc gtgggagacg
    13681 ccgtccggga cggtggacat cctactctgg ggtgtactgc aggtgggccg gtggggcgtg
    13741 ctcgaccacc gttcgggcgc actcagtacc gaagccggct ggcagccgaa agccgtgccc
    13801 gccttgaagc cctggctccg cgcgggcttc ttccacggcc acggcgacgg agacccgaac
    13861 gacactacgc acggcacctt cttccaggtt ctgccgaccc cccgcatcta cgcccgtttc
    13921 ccgttcttca acctgatgaa cagcgaggac ctttttggat cggtgacgct gcgcccgcac
    13981 aagacggtca gcatcaagac cgaagtgcac gggttgcggc tcgccagccg gaacgacctg
    14041 tggcttcttg gcggcggcgc attccaacct tggaccttcg gctacgtggg ccggccctcc
    14101 aacggcaagc gaggcctcgc caatctctat gacgcgagca tcgactggct ggtaagcccg
    14161 gtggccacgc tgagcgccta ctacggatac gccaccggcg gagcggtgat agagaaaatc
    14221 tacccgaacg gcaagaacgg ctcgctcggg tacctcgaat tgagctatcg attctagtcg
    14281 ccgggcggta gacgcagccg gagtccccgc tggcggttga ggctttccgt acgttcgcga
    14341 tccaccaacc ggagcggcac agctcggtca atcgcgcgag gcgttgcgta gtgtagtgtt
    14401 ccgccgtgaa cccagactgc ctcggcagct tcctccgttc catcccgaag cgcgatcaca
    14461 aactcggaag ccgtgctcct gatttcctct tcccaccggt agtcacgaac ttcgggtttt
    14521 gctggtggag gcggcggttc aggccggtac acttcggcgg gcgccggcgg tggcgtctgg
    14581 actacgacca cattttgcga cggctgggcg ccgccaacat aaccaccgta ccccccgtac
    14641 caagggaaca tggacgggta cccatacggc tgccagcgcg gccgttgaaa gcccggcacg
    14701 atgttttgcg gccggggcgt gtttccaatg ggcgggatgg gagacagcag aggaagtgac
    14761 gattcagaag gaaacgttcg gactgggttc acagcgcctc gctgctgggc gaacatgcat
    14821 gcactggccg ctacgaacat cgccgcgcct acaacgtgct tcacgccgtt gatccctctc
    14881 acttgcagct taccgctaat tcggccgtgg agccactcct aaccggcgct tttggcccac
    14941 ggtttgcaac agggccatgg tgattaaaga taaaaaggtc tgaataccat gaatcgagag
    15001 ctccctattg aaacccttgc cgatgaaatc tatagcgttg tgggccagca gcagggtaag
    15061 cgctacttga gggcggggga tctcacgcaa atgatgatgg agaggttcgg agaggagtgc
    15121 tcgaaggaca actgccgccg agccatccac cagttgattg attcgggccg ctgtgtttac
    15181 agctactttg gcgccagcta catcaccctt ccccacaaag caaagaagca accgtgacgc
    15241 atcttgagga cagatgcgtc acggaagtgc aactgactga aaaataaggt gtaagaaaga
    15301 atcagcgagc acccaatctc aggcgtagta ggccgcattt ttct
//
LOCUS       DATIFQ010000461        37625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_730572, whole genome shotgun sequence.
ACCESSION   DATIFQ010000461 DATIFQ010000000
VERSION     DATIFQ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 37625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37625
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730572"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VLE22_23170"
     CDS             <1..805
                     /locus_tag="VLE22_23170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17370.1"
                     /translation="IGSLALAAALFILQRRSAESAGLSGRLKRLEQENLELRARKAQF
                     EHQVQELQQQLGVESSSLAELASRGVQRSQLPAGTLDAIRMIGQLKESLASANVAAEQ
                     LRTRVAELEGELERVKDENRTLDTRQSELAEKLHGANSVVEAMQAELKGKNDRVVPLQ
                     MANKQLREENQTANEKISRVTQSVSQLEDINRRRDAFLTSILRRYRDVTDQYRALAAR
                     LENPGEGSAPTGTELSRIQSTISMAEDDLRQLNGLNVQADRLLRQIRGR"
     gene            complement(830..1294)
                     /locus_tag="VLE22_23175"
     CDS             complement(830..1294)
                     /locus_tag="VLE22_23175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSB17371.1"
                     /translation="MNCRKSRRGFTLIELLIVIAIIVILISAAAPKFSQILMNAKETA
                     AMRHIATIHTAQAQYSSQFGKYATVLSELGAPTSGSEGPAGANLIPDDLAKGVKGGYK
                     FTLEGTPTGYKVTAVPEAFGNTGRRTFFSDQTMVIRENWGQEPASANSPQVK"
     gene            complement(1411..3057)
                     /locus_tag="VLE22_23180"
     CDS             complement(1411..3057)
                     /locus_tag="VLE22_23180"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HSB17372.1"
                     /translation="MAKFIFVTGGVVSSLGKGIAAASIGCLLESRGLKVTLQKFDPYL
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTHSPLSQANNVTSGRIYEQIITRE
                     RRGDYLGKTVQVIPHVTNEIKAAMKKLAPDADVVIVEIGGTVGDIESLPFLEAIRQMR
                     HELGRENTMFLHVTLVPWIAAAHELKTKPTQHSVKELRAIGIQPDMLLCRSDHFLSQD
                     LREKIALFCDVNVDAVISVRDVDSVYEVPLVFTEQKVDEIILRLLRQDAGPRDMSSWS
                     EMVRRLKDPAGEVNIALVGKYVAYEDSYKSLKEALLHGGLAHNVRVSMQWTDSELLAE
                     RGCDELSAFDGILVPGGFGKRGVEGMIQAIRFAREAKVPYFGICLGMQTLVIEYARNV
                     CGLERADSTEFDPSTPHRVIFKLRELKGVDEMGGTMRLGAYRCMLAEGSFAHRAYGER
                     EISERHRHRYEFNREYERILTENGLRLTGQTPDGLYVEICEIPDHPWFLGCQFHPEFK
                     SRPLEPHPLFRAFVGAALEYRTKRLENQGALVEPRGFLHG"
     gene            complement(3071..3811)
                     /gene="kdsB"
                     /locus_tag="VLE22_23185"
     CDS             complement(3071..3811)
                     /gene="kdsB"
                     /locus_tag="VLE22_23185"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF009905.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HSB17373.1"
                     /translation="MPHKVLGVIPARFASSRFPGKALAAIAGKPMIQHVWERASQAGS
                     FSKIIIATDDERIAAAAKRFGAEVRMTRTNHASGTDRSAEVAASEDAGFVVNIQGDEP
                     LIDPTAIDAAVHGLAGALGTPMSTLKKRIEDPSELSNPNVVKVVTDLSGHAIYFSRSV
                     LPYVRGEQTPHYKHVGLYVYRRDFLLGYSLMPPGPLEQAERLEQLRALENGYRILVVE
                     TEYDSLGVDTPEDLERVSKVVEAKLAMS"
     gene            3958..4965
                     /locus_tag="VLE22_23190"
     CDS             3958..4965
                     /locus_tag="VLE22_23190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB17374.1"
                     /translation="MRALNRLIIPILGLALLPEGNGGAQVVPTPQGPEPRVSIEPRVK
                     TREAAEASGPRSNLRVDSTLVLIPVTVTDPLNRFVTGLDREHFKLEEDKVAQDISHFA
                     SEDAALSVGLVFDTSGSMGSKLQKSRQAAAQFFKTSNPDDEFFLVQFNDRPDLSMPFT
                     TNTEDIQTRMTFTQSKGRTALLDAVYLALHEMKKARNPRKALLILSDGGDNSSRYTES
                     EIKNLVREADVQIYAIGIFEPMAGRGRTAEEMAGPGLLSEIAEQTGGRHYPVDNINDL
                     PDIAAKIGIELRNQYVLGYTPTNKERDGKYRRVKVKLVQPRGLPPLRAFWRLGYYAPS
                     Q"
     gene            4949..5896
                     /locus_tag="VLE22_23195"
     CDS             4949..5896
                     /locus_tag="VLE22_23195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB17375.1"
                     /translation="MPRLNRFFCTLTFWVLLLPIVLPAQEDPVFRADTRLVVLHASVV
                     DRKGRLVTNLPQGAFKVFENGVEQQIRVFRREDVPVSMGLIVDNSGSMRDKRQKVEAA
                     ALQLVKASNRQDQVFIVNFNDEAFLDTPFTNDIKKLEEGLARIDSRGGTAMRDAISMS
                     IDYVKEAAKKDKKILLVVTDGNDNTSSITLEKLVQKAQQSEVLIYTIGLLDEEERREA
                     KRAKRAMDALTVASGGLPYYPKELAEVEQIALQVAHEIRNQYILAYTPTNEALDGSFR
                     QIRVTVDGPGRPVVRTRTGYYATPAAAGRTAAARPGMAQ"
     gene            5936..6700
                     /locus_tag="VLE22_23200"
     CDS             5936..6700
                     /locus_tag="VLE22_23200"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSB17376.1"
                     /translation="MQPRQGPLITVLSYNIHIGVGTDEKSALQRTAAVIRSVSPDLVS
                     LQEVDRMARRTGGVDQAAELAKMTGLRMAFGKALDQPGIDATGGEYGVAILTRLPIRW
                     FRTRALPFTRGYETRIVLEAELEWSKSTGEPIPLRFYATHFDNASADDRLASAKMLVE
                     LAGVNPVIPCILAGDLNAAPDSDPLKTLASSWGIAGAGRELPTFPATCPSRQIDYVLF
                     RPNERWKVIDTRVVDEPVASDHRPILAVLELLPGRR"
     gene            6963..8105
                     /locus_tag="VLE22_23205"
     CDS             6963..8105
                     /locus_tag="VLE22_23205"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSB17377.1"
                     /translation="MSTISTMTVPFYGHVRQYQNIKTEIDANVNEVLMSGQYVMGPML
                     KRFEKEFAAFHNTKYAVGVGNGTDAIWLVLMALGIGPGDECITTANTFFATAEAIWIA
                     GATAVFVDSDPLTNCIDPSKIEAAITPKTKCIVPVHLYGQCADMKAIRKIADKHNLHV
                     VEDNAQAICARGNGFQIGELSDAACTSFIIQKNLGTFGDGGAVVTNNEKIDQGVRRLR
                     NHGSLKRSVHSYGFNSRLDDIHAAVLSAKLKHIKEWSELRRKWAARYTAGLAGGKSFT
                     LPTETPGNYHVYHLYVIETKKAADRDVLLKFLEDNGIDAKCHYPIAIHQQEGYPWGKE
                     ARIAGPIPNSERNAACCISLPMFPELTAEEVDYTIAKVLEWDKAQA"
     gene            8115..9182
                     /locus_tag="VLE22_23210"
     CDS             8115..9182
                     /locus_tag="VLE22_23210"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17378.1"
                     /translation="MSDKYKVVVVGMGKRGMHHTLAFQADGRFEVAGVCDVDQSRLDA
                     AAAKLGAPVKGLDAAQVAAAVKPDVFCFCTMPTVRSQLIRIGIENGAKLIAFEKPVAM
                     NSIEGLKVKTLLDESGVKAVVSHQHRYGVHYRKVKEVIESGALGRVHTVYGTATGWLM
                     HMLSHLIDYMSWYNGEGAAEWVMAQAAGRGKFGDNHPSPDYIGGFVQFANGVRGIVEC
                     GAGAPDVPEVDYWWRKCRIGAQGPDGFAEVLTGGGWRAVTKTGTFSGEGSMNYDLDMP
                     PYIRQMAEWLDDDAKVHECNFASAWKGFEIMMALCRSAAEGGQVALPLAEPADELQML
                     KDRVADRKVLLSMAANAKEYL"
     gene            complement(9265..10326)
                     /locus_tag="VLE22_23215"
     CDS             complement(9265..10326)
                     /locus_tag="VLE22_23215"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSB17379.1"
                     /translation="MSKMLDEIRQQPDALERTLKSGLEAARRFRKRLGDRRPRFIILT
                     ARGTSDNAAQLGRYLLEITTGIPVSLSAPSIYTMYGASVDLKDALVVAVSQSGESTDI
                     NLVLERAVDQGAFTASITNESESTLAQLSKDVFLVRAGKEKSVAATKTYTGQVLMFYL
                     LAYALGAPIDLEELHRIPDWVMAALNAEPDVAKVVERYRFMTDTVVVGRGLNYANALE
                     FALKLKETCYVVAEGFSAADFLHGPIAMVEESFPVLLFGAGGPTWPSMKQMIERLHSQ
                     KAETLVITDRANTDVPAIANRSIVLPVKLADRNTWPADLYTPIPYIIPAQIFAALLAV
                     EKGHDPDQPRALSKVTRTM"
     gene            complement(10401..11996)
                     /locus_tag="VLE22_23220"
     CDS             complement(10401..11996)
                     /locus_tag="VLE22_23220"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HSB17380.1"
                     /translation="MRFLLAAAVVFVTATSLPGAEKSAEQLYREGQHAERSGEVVRAY
                     LLYSQAAAKNPAEPKYWLRSQTLRSRAVTVAKSLPKSDTALSPAGKQVLENGVPARIT
                     DDELAEARQPQAPMELKASTDRKDLDFRGDSRFLFEQVAAAYGLGAVFDGDYQPVAPF
                     RFRLDDADYREALNALQAATASFVVPVSERVFLVAKDNTQKRADLEPTVTVMEELAAP
                     VTIQEAQEAARAVQQALAITRLTVDTQRRLVLFRDRVSRVRPAQQLFRQLMRYPAVVS
                     IEVEFLELTRAEETSYGLSLQTMFPLVYFGDWFHSTPSIPSGFTKFLTFGGGKTLFGI
                     GVTDAAMFARMSRSLGRVLLRTEIRSTDGQAASIHVGDKYPIVTGGFLGFTEGQTSGF
                     PPQFNFEDLGLMLKVTPKVHGMEEVTLDLESEFKVLTGESFNDIPVIANRQMKSQIRL
                     RNGEWAVVAGMLVAQDTRSVTGPAGLSQVPGLGHLFREQTHNNDERRILLVLKPALLS
                     LPPDQLPTQAVWTGSENRPPAPL"
     gene            12130..13398
                     /locus_tag="VLE22_23225"
     CDS             12130..13398
                     /locus_tag="VLE22_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17381.1"
                     /translation="MSISTPTAQRPSFRPSVPDGLEKLGIPDVLVQDIVLRRVMIERT
                     STLQSLSRSLKLAPPVVEAVFRQMRHRQLVEVLGMVGNDYTFMLSGPGRQLATERFQM
                     SQYAGPCPVSLNEYLAGVRSQSAQVEVNRDKLRRAFSDLVLTDVLLDQLGPSVISQAA
                     LFLYGSTGNGKTSLAERLIRLYEDDVVLPYAVEVDNQIIILYDPVVHHRIPDNEDEMD
                     YDPRWVRCQRPCIITGGELVPSMLDLRMDEASGTYAAPLQMKANNGILVIDDFGRQLI
                     PPRDLLNRWIVPLDRRVDYLMLRYGVKFQIPFELMVVFATNLEPSSLADEAFLRRIQN
                     KILVEEVSAGVFDEILRRYAITKGIPFEQDKAEYLRSLCLRDGRTVLRACYPGDICRI
                     LVSISLYEGRAAQMTRSDLERAVNLYFARA"
     gene            complement(13445..13771)
                     /locus_tag="VLE22_23230"
     CDS             complement(13445..13771)
                     /locus_tag="VLE22_23230"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HSB17382.1"
                     /translation="MGSGSVPPVGGEARSGARKIFCVKFQREMEGLAEPPFDNHPLGQ
                     RIYENVSKEAWRLWVEHMKMIMNEYRLNLGTREAQEFLLKQMEEYFFGEGAAFPPDYV
                     PPGSTH"
     gene            complement(13840..14109)
                     /locus_tag="VLE22_23235"
     CDS             complement(13840..14109)
                     /locus_tag="VLE22_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17383.1"
                     /translation="MEAMDEQPASSEELLAQFNRLIRELLRGKIVRNTFRPWEIEILL
                     DIENCQIRESARENTLRRYQRAVQRQLEKGTGQLMKLSEFLEKKR"
     gene            14240..15994
                     /locus_tag="VLE22_23240"
     CDS             14240..15994
                     /locus_tag="VLE22_23240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17384.1"
                     /translation="MRIVVTLVFASTLAAQIPATDLRNTYTPNTDTHFAMPEYKTLAD
                     WEARRGELRRQILLAAGLLPLPEKTPLDPQIFGRLGRDGYTIEKVLLETMPGYYLAGN
                     LYRPRGRTGKLPAVLKPHGHWNYGRLENQPLFSGQAIAVNFALQGYVVFAYDMVGWND
                     TIQTPHAFGSPREQLWSFGPLGLQLWNSIRAVDFLESLPDVDAKRIGVTGASGGGTQT
                     FLLTAVDDRLAFSAPVNMISAIMQGGSPCENAPGLRVGAFNVEIASIMAPRPMMVVSA
                     TGDWTRNVPKEEFPAIRRIYELYGKTGNTDVIQIDAPHNYNQASREAVYRFFGKHVLG
                     AQEDSSFAEKEVREEKLQDLMVLHNRTLPAGALSFGQIFEQWQAMSRRQAEGVRDRDT
                     IRERLRLTIGVQWPKEVLDSVQGEQVVLSRAGKGDRVPGIWMDGRAPAALVVHPDGAD
                     AARNSREVRELKASGRAILLIDAFQTGSAVAPRDRSHRFFLTFNRSDDANRVQDILTA
                     LAFLKSKKPGEIELVGLDKAAIWSVFAAALAPEKVKLTAATDSFSGTDQEFLDRFFVP
                     GIQRAGGWKAALDLVAAR"
     gene            complement(16008..16859)
                     /locus_tag="VLE22_23245"
     CDS             complement(16008..16859)
                     /locus_tag="VLE22_23245"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSB17385.1"
                     /translation="MKFYKPNADFYVPDGISEAAALARTTHIAICAHQDDIEIMAYHG
                     IAECFGQDDKWFAGVVVTNGAGSPRAGIYEKYTDEEMQKVRRREQQKAAFVGDYACQI
                     QLDYTSSEVKDPKVAAVRDDLLAILKAAKPKVVYLHNPADKHDTHVGTMLRAIDALRS
                     LPPAERPEKVYGCEVWRDLDWLPDEDKQSLPVSARSNVAAALVGVFDSQVTGGKRYDL
                     ATAGRRLANATYFASHGVDVETALTFALDLTPLVNDASLSVAGYTTQFIEKLKADVEG
                     RLKKLGG"
     gene            complement(16889..18292)
                     /locus_tag="VLE22_23250"
     CDS             complement(16889..18292)
                     /locus_tag="VLE22_23250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17386.1"
                     /translation="MSSTIQISPKIKPPLDPGFVPASLWCRAYQTKVAESGQGKPLAI
                     ALARTDGSVSVFRTTVFPHEGAFVAANEVYVERLVKFLLWQRGGSKVTIAGDPRIAAY
                     IREVYSPSGKQSFDFHFMGEKVYGTPMVIEETSYEAAPAEHETTAALGRHLDGCRIGF
                     DLGGSDRKYAAVIDGEVVFSEEVVWDPYFQRDPQYHFDGVNDTLKRAASHLPRVDAIG
                     GSAAGVYVNNEVRVASLFRGVPPDLFDSRVRRLFYELKDAWGGIPFEVVNDGEVTALA
                     GSMALNDNGVLGVSMGTSQAGGYVTPQGNITNWLNELAFVPVDYRPNGPMDEWSKDYG
                     VGAQYFSQQAVARLIPAAGIEVSADMPLAERLNTVQKLMAGGDARARGIFESIGVYFG
                     YAIAHYASFYDLRHLLVLGRVMSGEGGDLILATAHKVLKAEFPELEESIQVHTPGEKE
                     KRHGQAVAAASLPQVRK"
     gene            complement(18357..19328)
                     /locus_tag="VLE22_23255"
     CDS             complement(18357..19328)
                     /locus_tag="VLE22_23255"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HSB17387.1"
                     /translation="MREIRVGTAQFEAKDGDKDYNLGVIGKLAGQAAREGVELVSFHE
                     CCISGYTFLENLSLEEITALAEPVPGGPSTGRLIEMSRELGCCLAAGLVERDGDRLYN
                     CYVVVSPDGFVAKHRKIHAFVSPHLTCGERYTVFELLGCKFGVLTCYDNNLPENVRIT
                     AMMGAEIILMPHVTGCLPSPMPGRGTVDPEIWRNREHDPVRCRQEFDGPKGRGWLMRW
                     LPARAFESGVFAVYSNPIGVEGDTIKPGGAMILDCFGETLAECRRLADEVVVATLDPA
                     KLAVAPGQSYIRARRPELYGLLVEPNPHLGPDKRPEIWWQKHRAASR"
     gene            19412..20740
                     /locus_tag="VLE22_23260"
     CDS             19412..20740
                     /locus_tag="VLE22_23260"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB17388.1"
                     /translation="MIGPLESCMTARSRIATVSWCLFDFANSSYTTLIITVAYAVYFR
                     DVVVNAKDNRGDQLWGIANFLAMLVIALTSPVVGALADWSGWKKRFLILSTLLAVAAT
                     ALMFFVGPGDIASGMLLFVLGTIGFEIGYVFYNAFLPDISTPRTIGRISGWGWAVGYA
                     GGLLCLLACRPWVAQPLRDAAGAISTAGVEAYQTSFLIVAGFYLLFALPAFTWLRESE
                     PQGRGVSWMESAAVGFRRVGETLSHLRRYRETAKYILASLFFTDGITTVVSFAAIYAT
                     TTIGFTNEEMVFLFLVMNVVAFPGALVAGYLADFLGARRTIILTLFLWIGVVLLGASA
                     ATKTVFWVMAFLAALGMGSTQAVGRSFMAQISPPSRESEFFGFYVLSGKFASMFGPLV
                     FGSISRFTGSQRAAVLSLLPFFLLGLALMYSINEGRAREAALLEGKTAAP"
     gene            20762..23428
                     /locus_tag="VLE22_23265"
     CDS             20762..23428
                     /locus_tag="VLE22_23265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSB17389.1"
                     /translation="MTLDFFERFRENLERFPGVVALQTLSDAGRETFTYQQAAAETRR
                     LSRYLQAKGLRPGDAAGILMENDARWGLAFLALQSAGAVVVPLDVLHQPQTLAGLIAH
                     AECRFLLIAPRFVPLLEQIQGLLPEPLPVLVCGEPAGSFPSWSEAPPAGDETPIPLIA
                     RDLDDPLVILYTSGTTGNPKGVVLTGRNIYRNVAEALQVIGPTHRDHLLSVLPLYHIL
                     ALAINLVIPLYLGARVTFLGALEAQKVFKALNEEGITIFICVPQFFYLVHRRIFQEVE
                     RQRRVKRFLFRRLLAFSRFANLHLGWNPGRFFFRQIHEKFGPSLWLFGVGGARFDVEV
                     AGDFRDLGFKLAQAYGMSETAALITVAPPVAEAVGSVGKPLPHVEIRIDQPDENGVGE
                     VLTRGENVMLGYWKNTEATAEAIRDGWLYTGDLGYIDSHGWLYITGRKKDVIVLSSGK
                     NIYPEEIEYFYQSDCPLIKEMCVLGVPDPGAGEEQEVLHAVIVPDFDALRDRQVVNVF
                     EALRYEIESLSQRVPPYKRVRSLEIRTEALPRTTTRKLRRFQVQQELGQRTAAASAPA
                     VTPEPEPDDPVERRVRDLIRRMKDAPSIRGAMNLELDLGFTSLERAELMFGVQETFGL
                     RIPDEEATGVFTVDDLVGAVTRRLSGELREDGQARASWSDLLRAPLTPDEERMLRAQL
                     ARRPVVEVVYYLLARIARVVVRVLLRFRVRGVERLPNQYPFLICPNHLSFIDAFLVMS
                     TLPFRVLRRLCILGDSRYFSSGVTALVGRLGKVIPVNPDRGPQVSLRLAAEGLRRGLV
                     LCVFPEGERSIDGKLKPFRPGASILAAEFGVPVVPAGIRGAWEVWARGSGRIRLHPVS
                     LEFGQPLQPPAPGTPYETVTRLLQEEVRRLVE"
     gene            complement(23432..25225)
                     /locus_tag="VLE22_23270"
     CDS             complement(23432..25225)
                     /locus_tag="VLE22_23270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB17390.1"
                     /translation="MNFRRLSLLWKIWLSTSVALTALFAVTGWVVQRHVLETSARNLE
                     EEVQAGFQAYASLWRARAEMLRSVASIISSLPNVRAAFGTRDQATMRDAAQEVWARVS
                     EDLRETAFFVVTDPKGNTIASLGEGASAAPRSWPVVPDARSKFPEQVSGFFLENNELV
                     QLVLTPVYVDSGRGPALISVLVTGYAVNHPVAQRLKAATGGSEFVFLSQGRVFASTLN
                     DRASQELGKAVAVHTPGSRVSDGVYEYMPVRRDLIDVHGKPIGALYILRSFEGARQRV
                     AALRRDVILIWLLAVVAGLVLTHLFARKIVEPVKKLDEAAAELARENYSYRIGVESEG
                     EDELGRLAATFNKMCQSLESARQELIRRERISTIGRLAGSIVHDLRNPLAAIYGGAEM
                     LVDTELAPPQVKRVASSVYKASRRIQELLQDLVNVTRGKSGRAEMCVLREVIVDAADQ
                     VRAVAERQAVSLAIDLPGELELPLERGRMERVFLNLFSNALEAMPGGGTITVSASRNG
                     EVALVEVRDTGPGISPQIRDQLFQPFVTFGKKNGLGLGLALSRQTVLDQGGDLWVESE
                     PGRGARFLIRLPLVRNSAVETLDNGQSGRIG"
     gene            complement(25222..25899)
                     /locus_tag="VLE22_23275"
     CDS             complement(25222..25899)
                     /locus_tag="VLE22_23275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB17391.1"
                     /translation="MSFCALAATVGGRVELVDSRDPQVRRHRNFSGVVVWLDPVHGDP
                     PRTEPRTAKMLQQGKRFVPHILAIPVGTTVEFPNLDPIFHNAFSNFSGQPFDTGLYPP
                     GMTQKVTFRRDGVVRVFCNIHSTMSAVIVVTKTPYAAVTDRRGDFQIEGVPAGDYQVR
                     VFHERATEESLRALERKITVGGETIGLPLIRVSESGYLEVPHKNKYGRDYPEPPPDHM
                     VYPGVRR"
     gene            complement(25907..27256)
                     /locus_tag="VLE22_23280"
     CDS             complement(25907..27256)
                     /locus_tag="VLE22_23280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17392.1"
                     /translation="MLSRRKSFRPRKGRLFLAVCLLLPGVAAAQTPAEWKTVLERLDR
                     LESENRSLREEVGELRRLVSDGGPQSGVEERLAIQEERIREQAQTKVESSQRLPLRVT
                     GMALFNAFLNGRASGGADNPPLAARISGSAAGGASLRQSVVGLQYEGTSTVWGGKVRG
                     SLYADFFGGTGDSLNQQARIRTGSIELEWRSRSILAGQEKPIFAPREPNSLAQVGISP
                     LTGAGNLWRWQPQIRVEQRFRLGQHARFSAQLGVLQTSEETTAVPSDYAPTVARRRPG
                     FEGRFLFSKDLDHTRRLEIAPGFHTSTSHVAGTSIPSNLFSLDWFANPWRKLEFTGAF
                     FSGQNVGHFGSPRQGFTVISEYLVIPVHSRGGWAQLSLLATQRLSFNLMGGRHDDRNR
                     DLLPGQVGKNTAGAANVMYRLSPNVIVSLEASQVRTDYLGVGTRINNHYDLALAYLF"
     gene            complement(27240..27629)
                     /locus_tag="VLE22_23285"
     CDS             complement(27240..27629)
                     /locus_tag="VLE22_23285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB17393.1"
                     /translation="MPRKVLIVEDTPYYASALEIALSSLPDVVVSHAPTGGAALEQLE
                     ALEPGTACVILTDLHMPLMDGFELIERLRSGDRHERLPVIVLSGDTDPETPGRIRRLG
                     ADAFFAKPYSPTEVRNKVEELLNAFAP"
     gene            complement(27699..28373)
                     /locus_tag="VLE22_23290"
     CDS             complement(27699..28373)
                     /locus_tag="VLE22_23290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB17394.1"
                     /translation="MQTVLVIDDDENLRETIAVMLEQEGFRPVPAADGKDGFEKALTL
                     KPDMILVDLRLPLMTGTEICKQLRAAKIQTPIIVMSAVGDEIDKVLLLEIGADDYVVK
                     PFGTRELLARIRAVLRRTVADARKVLHFSDVEVDVERRIVTRKGEEVKMTPAEYNLLA
                     YFLHNPDRALTRDMILNSVWGYESFPNTRTVDVHVVKLRQKLEPDASVPKHFLTVHGV
                     GYRFIP"
     gene            complement(28425..29522)
                     /gene="arsB"
                     /locus_tag="VLE22_23295"
     CDS             complement(28425..29522)
                     /gene="arsB"
                     /locus_tag="VLE22_23295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HSB17395.1"
                     /translation="MEQTIAQPQAVEKRLSLFERYLTLWVGVCMLAGVLLGKLTPGLV
                     DTLRRMEFGSGSQINVPIAVLIWLMIIPMMMKVDFTSIRNVGRRPRGLLVTLFVNWLV
                     KPFSMALIGWIFFRVVFSGWISPAEADQYIAGVIILAAAPCTAMVFVWSYLTDGDPAY
                     TLVQVSVNDLIMLVLFAPIVRFLVAGASSLSVPFLVLLYSVIAFIVIPLTVGTLLRAW
                     LIRRHGKEWFETSLLPRFAPVTIAALLATLVFIFAFQADNITGKSFHVVLIAIPILIQ
                     VYFNSSLTYGLMRLFRVEYSVAAPGALIGASNFFELAVATAIALFGPGSGAALATVVG
                     VLVEVPVMLSVCAACNRTQHWFDFRSRPADG"
     gene            complement(29548..31335)
                     /gene="arsA"
                     /locus_tag="VLE22_23300"
     CDS             complement(29548..31335)
                     /gene="arsA"
                     /locus_tag="VLE22_23300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HSB17396.1"
                     /translation="MRTAEFLQTATRILFFTGKGGVGKTSLACATAVALAGCERRVLL
                     VSTDPASNLDEVLGVTLSGAPVAVPGADGLFALNIDPEAAAAAYRDRVVGPYRDILPE
                     AAINSMEEQLSGACTVEIAAFDEFTKLLGDPDATQGFDHIIFDTAPTGHTLRLLKLPA
                     AWTGFIATNTTGTSCLGPLAGLQAQKALYDASLKTLSDATSTTFVLISRPETSALAEA
                     ERTRTELADIGVSNQYLFLNGVFIARDQSDPAARALEQRGREALAKTPDGLRLLPTFE
                     VPLLPYAPMGIQNLRAVFTVGGAGYAPVEHSSGLTASPSPPLADLVDQIEKAGHGVVM
                     TMGKGGVGKTTVASAIAVELAHRGHRVHLSTTDPAAHLMYTIGTAVRGLSVSRIDPAV
                     ETRMYSEQVMAKSAPNLDEQGIALLEEDLRSPCTEEIAVFSAFAQTVARGEDEFVVLD
                     TAPTGHTLLLLDAAESYHREVSRTMSDTPDTVRRLLPRLRDDSFTRVLLVTLPEATPV
                     HEAARLQDDLLRAGITPFAWVINQSFFQDGFRDPVLVDRGTRELPFIAEVRDHLAGRF
                     ALVSWKPEAPLGPERLRELASERPVCAAQ"
     gene            complement(31347..31712)
                     /gene="arsD"
                     /locus_tag="VLE22_23305"
     CDS             complement(31347..31712)
                     /gene="arsD"
                     /locus_tag="VLE22_23305"
                     /inference="COORDINATES: protein motif:HMM:NF018637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HSB17397.1"
                     /translation="MKKLEVFDPPMCCSTGVCGPNVDPALVQFGSDFLWIAGQGVHVE
                     RYNLAQQPQAFAFNEVVKALLHEHGNTCLPLVLLDGEVISIGRYPSREELANAAGLDL
                     GIRKPDAAGLRIVQRRCCG"
     gene            complement(31753..32106)
                     /locus_tag="VLE22_23310"
     CDS             complement(31753..32106)
                     /locus_tag="VLE22_23310"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSB17398.1"
                     /translation="MKAPRKDPIACCDTPELELEADLRPVEGAAADEELATLAKAVAH
                     PARVRILRLLSHKEARVCSQIVGELPLAQSTVSEHLRILKEAGLIRSREEGSRVGYCI
                     NFPALRRLKALVAIL"
     gene            32234..32950
                     /locus_tag="VLE22_23315"
     CDS             32234..32950
                     /locus_tag="VLE22_23315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17399.1"
                     /translation="MKARRSIVVLVALLGARLFAADFWEKKRFPDWSAKEIRKLLTDS
                     PWARPVLVPLETLRGQQATSMPHTQAKGACHQCSVGGTPVQLQSSDIPELAHPLTVTV
                     RWHSALPLKQALAKLLFGDEVSTSAAAAELLSREDDSYVLGLSSLPRQVLPEDLESIK
                     SGAVLRVRNGPSIPAREARVRAEGDMVNLLLYFPKPQRDAAGITLEHREVELGVKLFA
                     IEIKRKFKLSEMVYQGKLEL"
     gene            33031..33414
                     /locus_tag="VLE22_23320"
     CDS             33031..33414
                     /locus_tag="VLE22_23320"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSB17400.1"
                     /translation="MKKVSGNDVSRYAAMLAAMGTEPRLRIMRLLLSAHPGGMVVGDI
                     QTELGTSASTLSYHLEKLKNEGLIRVRRQGTFLWHTANTDALQELLNFLYAECCGRTQ
                     TVNIEDLVSTKPLKQAALAPREPEN"
     gene            33429..34262
                     /locus_tag="VLE22_23325"
     CDS             33429..34262
                     /locus_tag="VLE22_23325"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HSB17401.1"
                     /translation="MDIKQAVKERYGKAALQVTGGGAPCCGGNSSGAGCGDPITSNLY
                     DEKQTGELPETAVLASLGCGNPTALAQLNPGETVLDLGSGGGIDVLLSAKRVGPAGKA
                     YGLDMTDEMLALARENQRKAGVTNVEFLKGEIENIPLPDASVDVIISNCVINLSADKD
                     RVLREAFRVLKPGGRFAVSDVVMRGEVPSAVRRNIELWAGCIAGALEENEYRAKLTAA
                     GFGDVAVEPTRVYRVDDAREFLTASGIDVEAAAAAVDGKFMSAFVRAVKPAEAGRATA
                     Q"
     gene            34274..34678
                     /locus_tag="VLE22_23330"
     CDS             34274..34678
                     /locus_tag="VLE22_23330"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HSB17402.1"
                     /translation="MLILCTGNSARSQMAEGLLRHLAGGRYEVFSAGTHPSFVRTEAI
                     AAMHELGIDISGHRSKSVDEFRLQAFDYVITVCDNARESCPVFPGNARRIHWSFEDPA
                     AAVGDQAVRLEAFRRIRDQIADRLRDFVQAAA"
     gene            34736..35386
                     /locus_tag="VLE22_23335"
     CDS             34736..35386
                     /locus_tag="VLE22_23335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17403.1"
                     /translation="MKRRIYLLMSKLLLTALLATTALFGQYKLESTGAPPDELAPAVK
                     EALQASGAKIVGADGSALCEIWFRKSLPSGPASTEQGVSLKTVPHGAMLGVIRFPKRG
                     QDRRGLTIPEGVYTLRYSLFPPDGNHQGVAAQRDFLLLVPAAADTDVNSTPSFKEVVA
                     MSAKTTGGSHPASLSLWKDEAGHEPGLAQEGESDWVLHTKIGDTAVGLIVVGVFQG"
     gene            complement(35427..36317)
                     /locus_tag="VLE22_23340"
     CDS             complement(35427..36317)
                     /locus_tag="VLE22_23340"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB17404.1"
                     /translation="MDRRTFVAASGAALAGRTASGQTTAPPKKAKITSSVMLWTLKGT
                     FDEKMEAAARSGVQSVELVGEHINWSETEVDRVKKLARSFGLGMDTIIATPNWKNRPV
                     SMVDPAQRENFLNDVRQAITFAQKLEIPQIILMSGDEIPGRSREEQYASLLEGTRRAG
                     ELAAKANVTLIVEPLNAKVNHKGFFLTTCAEGLKLVREVDNQNVRLLFDIYHEQVQVG
                     NVIRTAVEAAPYVAVYHVADNPGRNDPGTGELNYANIYKSIQKAGYGGYITMEYLPVG
                     EQVGSLTKALNEMRAALSTT"
     gene            36361..>37625
                     /locus_tag="VLE22_23345"
     CDS             36361..>37625
                     /locus_tag="VLE22_23345"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HSB17405.1"
                     /translation="MRQLRYTTRSGIDVVRTVSRVPFRKGLDHLLRELDSFRGIYLSS
                     GYEYPGRYSRWDIASTRPPLEIVAYGRRIEFRALNVRGEILTQMLYPALAGHPHWESL
                     EPCGGSLVGRLKPLPELFPEEERSKQPSAFSILRALIEEFRNEEDSRLALIGAFGYDL
                     LFQFDPIRLRLPRSGQKDLHLFLCDDIYFMDRKKEQIERYQYDFEKDGLSTLALDHSA
                     EPVSPPGRSSIEGIASDHAPEEYMANVEKVRQGMKQGDYYEVVLRQTFRAPYDGSQSE
                     LFRRMQETSPSPYEFFLQFGDEQLIGASPEMFVRIEGDRVETCPISGTARRTGDPMHD
                     ADSIRQLLNSAKEESELTMCTDVDRNDKSRVCAPGSVKVIGRRLIESYAGVFHTVDHV
                     EGILQPGYDALDAFLSHMWAVTVIGAPKKA"
BASE COUNT         7101 a        11782 c        11474 g         7268 t
ORIGIN      
        1 gatcggctcc cttgccttgg ccgcggcttt gttcattctt cagcggcgca gtgcggagtc
       61 tgccggcctg tccgggaggc tgaagcgcct ggagcaggag aacctcgaac tccgtgcgcg
      121 gaaagcacag ttcgagcacc aagttcagga gctccaacaa cagctcggcg tggagtcgtc
      181 gtccctggcc gagcttgctt cgcgcggcgt ccagcgaagt cagttgcccg cgggcacgct
      241 ggatgccatc cggatgattg gccagctcaa agagagtctc gcttcagcga acgtagcggc
      301 cgagcaattg cgcactcgcg tggctgaact cgaaggagag ttggagcgcg tgaaggacga
      361 gaaccgcacg ctcgacaccc gccagtccga gctggcagag aagctgcacg gcgcaaattc
      421 cgtggtggag gcaatgcaag ccgagctgaa gggcaagaat gacagggtgg tgcccttgca
      481 gatggccaac aaacagctcc gggaagagaa ccagacggcg aacgaaaaga tctcccgggt
      541 tactcaatcg gtcagccagc ttgaggatat caaccggcgg cgcgacgcct tcctgaccag
      601 catcctgcgg cggtatcgtg acgtgaccga tcagtaccgc gctcttgcag cccgcctgga
      661 gaacccaggc gaaggctccg ctcccaccgg tacggaacta tcccggatcc agagtacgat
      721 ctccatggcc gaagacgatc tgcggcaatt gaacggcttg aacgtacagg ccgaccgcct
      781 actgcggcag atccgcggcc ggtaagaacc agcggccgaa ctgaccgcct tacttcactt
      841 gtgggctgtt ggctgacgcg ggttcttgtc cccagttctc gcggatcacc atcgtctgat
      901 cggagaagaa ggtccggcgg ccagtgttcc cgaacgcctc gggcacggca gtgaccttgt
      961 acccggtggg cgtaccctcc aacgtaaact tgtagccgcc cttgacgccc ttcgccaggt
     1021 cgtcgggaat gaggtttgcc cccgccggtc cctccgaacc gctagtcggc gcgccgagct
     1081 cggagagcac cgtcgcatac tttccgaatt gcgatgaata ctgggcttgc gccgtgtgga
     1141 tggtggcgat gtgacgcatc gccgctgtct ccttggcgtt catcaggatc tggctgaatt
     1201 tgggcgccgc cgcgctgatc agaatcacaa tgatcgcgat cacgatcagc aactcgatca
     1261 gcgtgaaacc tcggcggctc ttcctgcagt tcatggcgcc tctcctcctg ctcgttgccg
     1321 ggctgcgact cggcgcgcta gccgtgaccg caggacggcg gagtccatcg gcactcctgg
     1381 gcccataata gcgttgacgc tctgtccccc tcaaccgtgg aggaatcctc ggggttcgac
     1441 gagggcgccc tgattctcca accgcttggt gcggtattca agcgccgccc cgacgaacgc
     1501 ccggaacagg gggtgcggct cgagtgggcg cgacttgaat tcggggtgga actggcagcc
     1561 gaggaaccag ggatggtccg ggatctcgca gatttcgacg tacagcccat cgggagtctg
     1621 accggtgagc cgaagcccgt tttcggtcaa gattcgctcg tactcgcggt tgaactcgta
     1681 gcggtgccgg tggcgctcgc tgatttctct ttcgccatag gcgcggtgcg cgaagctccc
     1741 ctcggcaagc atacagcggt aagcgcccag ccgcatcgtc ccgcccatct cgtccacgcc
     1801 cttcaattca cgcagtttga agatcacacg gtgcggcgtc gacggatcga attcggtcga
     1861 atccgctcgc tcgagaccgc acacattccg cgcatactcg atgaccaaag tctgcattcc
     1921 tagacaaata ccgaaatagg gcactttggc ttcgcgggcg aaccggatgg cctggatcat
     1981 gccctccaca ccgcgctttc cgaaacctcc cggcacgaga atgccgtcga aggcgctgag
     2041 ctcgtcacat cctctttcgg caagcaattc ggaatcagtc cactgcatac tgacccgcac
     2101 attgtgcgcc aggcctccgt gcagcagcgc ctccttcagg ctcttgtagg agtcctcgta
     2161 ggccacgtac ttaccgacga gcgcgatatt cacctctcct gccggatctt tcaaccggcg
     2221 caccatttcc gaccaggagg acatgtcccg cggtcccgca tcctgccgaa gcagacggag
     2281 aatgatttcg tcgaccttct gttcagtgaa gaccagcggg acctcgtaca cggagtccac
     2341 gtcgcgcacg ctgatcacag cgtccacgtt gacatcacag aagagggcga tcttctcgcg
     2401 caagtcctga gacaggaaat gatccgaccg gcacagcagc atgtccggct ggataccgat
     2461 cgcccgtagt tccttcacac tgtgctgcgt gggcttcgtt ttcagctcgt gagccgccgc
     2521 gatccacggc accagggtga cgtgaaggaa catcgtgttc tcccggccca gctcatgcct
     2581 catctggcga atcgcctcga ggaatgggag cgactcgatg tcgcccaccg ttccgccgat
     2641 ttccacgatc acaacgtcgg catcgggcgc cagcttcttc atcgccgcct tgatttcatt
     2701 cgtgacgtgc gggatgactt gaaccgtctt gccgaggtag tcgccgcgcc gctcgcgggt
     2761 gatgatctgt tcgtagatgc ggcccgaggt gacgttgttg gcctgcgaca gcgggctgtg
     2821 tgtgaaccgt tcgtagtggc cgagatcgag gtccgtctcg gcaccgtcgt cggtgacgaa
     2881 cacctcgccg tgctggaacg ggctcatcgt gcccggatcg acgttcagat acgggtcgaa
     2941 cttctgaagc gtcactttca gcccgcggct ttcgagaagg cacccgatcg aagccgccgc
     3001 aatccccttt cccagggagg aaacaacacc gccggtgacg aaaataaact tggccatcgc
     3061 tgtcgtacgc ttaactcatc gcaagttttg cctcgactac tttcgagacg cgttccaggt
     3121 cctcgggcgt atccacgccg agtgaatcgt attctgtctc caccaccaga atgcggtatc
     3181 cgttctcaag tgcgcgcaac tgctccagac gctcggcctg ctccagcgga cccggaggca
     3241 tcagcgagta accgagcagg aaatcgcgcc ggtagacgta gagtcccacg tgtttgtagt
     3301 gtggcgtctg ttcaccccgc acgtacggga ggactgaccg tgagaaatag atcgcgtggc
     3361 cggagagatc cgtaactacc ttgaccacgt tggggttgga aagttcagag gggtcctcga
     3421 tgcgcttctt gagcgtgctc atcggtgtac ctagcgcacc cgccaacccg tggacagccg
     3481 cgtcgatcgc cgtcgggtcg atcagcggct catcaccttg aatgtttacc acaaagccgg
     3541 cgtcttccga cgccgccact tcggcggagc ggtccgtgcc cgacgcatga ttcgtccgtg
     3601 tcatccggac ctccgccccg aacctcttcg ccgccgcggc gattctttcg tcgtcggtgg
     3661 cgatgatgat tttcgagaag ctgccggcct gcgacgcccg ctcccagacg tgctggatca
     3721 tgggctttcc ggcgatggcg gcgagagctt ttccggggaa tctcgaagag gcaaaacgcg
     3781 ctggaatgac gcccagaacc ttgtgcggca cagacgatga taccatgaac gccgggaggg
     3841 tgtggccgcc ctccgcgggg tcgaatcagg ttaattctca ggccgattcc ggagagcgag
     3901 caactcgcat gttatactga tggtagccgc gccacacact ggagagccgt catggtcatg
     3961 agggcgctca acagactgat catccccatt ttgggactgg ctttgctgcc ggagggcaat
     4021 ggcggcgctc aggtcgtgcc cacgccgcaa gggcccgagc cgcgggtttc catcgagcca
     4081 cgagtgaaga cacgggaagc agcggaggct tccggcccgc gcagcaacct ccgggtcgac
     4141 agcacgctgg tgctgatccc ggtcactgtc accgacccgc tgaaccgttt tgtgaccggc
     4201 ctggaccggg aacatttcaa actggaggag gataaggtcg cgcaggacat cagccacttc
     4261 gcgagcgagg acgccgccct ctcggtgggt ctagtcttcg ataccagcgg cagcatgggg
     4321 agcaagctcc agaaatccag gcaggcggcg gcgcaattct tcaaaacctc gaatccggac
     4381 gacgagttct ttctggtgca gttcaatgac cgccccgacc tcagtatgcc cttcaccacc
     4441 aacactgagg atatccagac gcgcatgacc ttcactcagt ccaagggaag gaccgcgttg
     4501 ctcgacgccg tctacctcgc gctgcacgag atgaagaagg cccggaaccc gcgcaaggcc
     4561 ctgctcattc tttccgatgg cggcgacaac agcagccgct ataccgaaag cgagatcaag
     4621 aacctcgtgc gcgaggccga cgtgcagatc tacgcgatcg gaatcttcga accgatggcc
     4681 ggccgcggac ggacggccga agagatggcg ggccccggtc tactcagcga gatcgccgag
     4741 cagactggcg ggcgtcacta tccggtggat aatattaacg acttgcctga tatcgccgca
     4801 aagattggga tcgaactccg aaaccaatac gtactcggtt atacgccgac caacaaggaa
     4861 cgagacggaa agtaccgccg cgtgaaggtg aaactggttc agccgcgcgg gctgccgccg
     4921 cttcgtgctt tctggcggct gggatattat gccccgtctc aatagattct tctgcaccct
     4981 gactttttgg gtgcttttgc ttccaatcgt tttgccggcg caggaggacc ccgtctttcg
     5041 cgccgacacc cgtctcgtcg tgctccacgc gagcgtcgtc gaccggaaag gccgcctggt
     5101 caccaactta ccgcagggtg cgttcaaggt cttcgagaac ggcgtcgagc agcagattcg
     5161 agtattccgc cgcgaggacg tgcccgtgtc gatgggcctc atcgtagaca acagcggcag
     5221 catgcgcgac aagcggcaaa aggtcgaggc ggccgctctt cagctcgtaa aggcttccaa
     5281 ccggcaggat caggtgttca tcgtcaattt caacgacgaa gctttcctcg acacgccgtt
     5341 cacaaatgac atcaagaagc tcgaagaggg tcttgcgcgc atcgattcgc gaggcggcac
     5401 cgccatgcgc gacgccatca gcatgtcgat cgactacgtt aaggaagcgg cgaagaaaga
     5461 caagaagatc ctgctagtgg tcaccgacgg caacgacaat acgagcagca tcacgctcga
     5521 gaagcttgtg caaaaggcgc aacaaagcga ggtgctgatt tacacgatcg gccttctgga
     5581 cgaggaggag cgccgcgagg caaagcgtgc caagcgcgcg atggacgctc tgacggtggc
     5641 ctcgggcggc ctgccgtact atcccaagga actcgccgag gtggagcaga tcgctctcca
     5701 agtcgcccac gagatccgta accagtacat tctcgcgtac acgccgacaa acgaagcgct
     5761 cgacggcagc ttccgtcaga tccgagtcac ggtggacggt ccggggcgcc cggtagtccg
     5821 cacccgcacc ggttactatg ccactccggc agcagccggc cggaccgctg ccgcccgccc
     5881 cggaatggca cagtgagggt cgcggcggag accgcgatcg aaacaggagc aggtcatgca
     5941 gccgcggcag ggcccgctga tcaccgttct tagctacaac atccacatcg gcgttggcac
     6001 cgacgagaaa tcggcgctcc agcgcactgc ggcagtcatt cgttcagttt ctcccgacct
     6061 cgtttcattg caggaagtcg atcgcatggc ccggcggacc ggaggcgttg atcaggcggc
     6121 cgaactggcg aagatgacag gccttcgcat ggcgttcggc aaggcccttg accagcctgg
     6181 gatcgacgcg acgggcggtg agtacggtgt ggcaattctc acccggctgc caatccggtg
     6241 gtttcgaacc cgtgcgttgc cgtttacccg cggctatgag acccggatcg ttcttgaagc
     6301 ggaactggag tggagcaaga gcacgggtga gccaattcct ctgcgtttct acgcaaccca
     6361 cttcgacaac gccagtgcgg acgaccggct tgcatccgcc aagatgctgg tcgaactggc
     6421 gggcgtcaat ccggttattc cgtgcatcct ggcgggtgat ctcaatgcag cgccggacag
     6481 cgatccgctc aagacgctcg catcgtcgtg gggaatcgcc ggcgcgggcc gcgaactgcc
     6541 cactttcccc gccacgtgcc cgagccggca gattgactac gtcctgttcc gcccgaatga
     6601 acgttggaag gtcatcgaca cgcgcgttgt ggacgaaccc gtggcttccg accatcgacc
     6661 gatcctggcg gtgctggaat tgctgccggg gcgtcgctaa cggcgagcgc gaagctccac
     6721 tttcatccgg tgtatcaatt cgagtacact ggtttgtctg aggctgcggc ggccctcata
     6781 cccttgagcg tctttgcagg acgggagcag cctcgtccct cggcctcgtg cggcataccg
     6841 gggcccgagc aaccccgacc accactccgg tgaagtggat aacctttcca ctcggaaggc
     6901 cccgggaaag atgtaatcct catggaatac tgccagtgac agcaaaggag agcgcaatcc
     6961 agatgagcac gataagtacg atgactgtgc cattttacgg ccacgtccgt cagtatcaga
     7021 acatcaagac ggagatcgac gccaacgtaa acgaagtcct catgagcggc cagtacgtga
     7081 tgggcccgat gcttaagcgg ttcgagaagg agttcgccgc gtttcacaac accaagtacg
     7141 ccgtcggcgt cggcaacggc acggacgcca tctggctggt gctgatggcc ctcggcatcg
     7201 gtcccggtga cgagtgcatc actacggcca acaccttctt cgccaccgcc gaagcgatct
     7261 ggatcgccgg cgccacggcc gtcttcgtag actccgaccc gttgacaaac tgtatcgatc
     7321 cctccaagat cgaggcggcc atcacaccga agacgaagtg catcgtcccg gttcacctgt
     7381 acggccagtg cgccgacatg aaggccatcc gcaagatcgc cgacaagcat aaccttcacg
     7441 tcgtcgagga caacgcccag gccatttgcg cccgcggcaa cggattccag atcggcgagt
     7501 tgagcgatgc cgcctgcacc agcttcatca tccaaaagaa cctcggcacc ttcggcgacg
     7561 gtggagcggt cgtcaccaac aacgaaaaga tcgaccaggg cgtccgccgg ctccgcaacc
     7621 acggttcgct caagcggtcc gtccacagct acggattcaa cagccggctg gacgacattc
     7681 acgccgccgt actcagcgcc aagctgaagc acatcaagga gtggagcgaa ctgcgccgca
     7741 agtgggccgc gcgctacacc gccggcctgg caggcggcaa gagcttcacc ttgcccaccg
     7801 aaacgccggg caactatcac gtctaccacc tctacgtcat cgagacgaag aaggccgccg
     7861 accgcgacgt actgctcaag ttcctcgagg acaacggcat cgacgccaag tgccactacc
     7921 ccatcgccat ccaccagcag gaaggttatc cgtggggcaa ggaagcgcgc atcgccggcc
     7981 cgattccgaa ctccgagcgc aacgccgcct gctgcatctc actgcccatg ttccccgaac
     8041 tcacggccga ggaagtggac tatacgattg ccaaggtcct ggagtgggac aaggcgcagg
     8101 cctgaggggc gcgaatgagc gacaagtaca aggttgtcgt tgtgggcatg ggtaagcgcg
     8161 gcatgcacca taccctcgcg tttcaggctg acggccgctt tgaggtcgcc ggcgtctgtg
     8221 acgtggacca gtcgcgcctc gacgcggctg ccgcgaaact cggcgccccg gtgaagggcc
     8281 tcgacgccgc acaggtggcc gccgccgtga agccggacgt gttctgcttc tgcactatgc
     8341 cgaccgtccg ctcccagctc atccgcatcg gcatcgagaa cggcgcgaag ctgatcgcat
     8401 tcgagaagcc ggtggcgatg aacagcatcg aggggctgaa agtgaagacg ctgctcgacg
     8461 agagcggcgt caaggcggtc gtcagccacc agcatcgtta cggcgtccac taccgcaaag
     8521 tcaaggaagt catcgagagc ggagctctag gccgcgtcca caccgtatat ggcacggcca
     8581 ccggctggct gatgcacatg ctttcgcacc tcatcgacta catgagctgg tacaacggcg
     8641 agggcgcggc cgagtgggta atggcgcagg ccgccggacg cggcaagttc ggcgacaacc
     8701 atccgtctcc ggactacatt ggcggctttg ttcagtttgc caatggcgtc cgcggcatcg
     8761 tcgagtgcgg agctggcgcc ccggacgtgc cggaagtcga ctactggtgg cggaagtgcc
     8821 gcatcggcgc tcagggaccg gatggctttg ccgaagtgct taccggcgga ggctggcggg
     8881 cggtaaccaa gactggaacg ttttccggcg aagggtcgat gaactacgac ctcgacatgc
     8941 cgccgtacat ccggcagatg gcggaatggc ttgacgacga tgcaaaggtt catgagtgca
     9001 atttcgcaag cgcatggaag ggcttcgaga tcatgatggc cctctgtcgt tcggccgctg
     9061 aaggtggtca ggtcgcgttg ccgctggccg aacccgccga cgaattgcag atgctgaaag
     9121 accgggtcgc cgaccgcaag gtgctgctct ccatggcggc caacgccaaa gaatacctgt
     9181 agatacagcg tccctcaaat tcagaactca ggagtgattt cgacatcctg agttctgaat
     9241 tctttcggcc cgcgttctgc cgcctcacat cgtccttgtc actttgctca gcgccctcgg
     9301 ctggtcgggg tcgtggccct tctccacggc gagcagggcc gcgaaaatct gtgcggggat
     9361 gatgtatgga atgggggtgt acaggtcggc cggccaggta ttccggtcag cgagtttcac
     9421 cggcaggacg atggatctgt ttgcgatcgc cggcacatcg gtgttggccc tgtcggtgat
     9481 gacgagcgtc tcggccttct gcgagtgcag tctttcaatc atctgcttca tcgaaggcca
     9541 cgtcgggccg cccgctccga acagcagcac agggaaggac tcttccacca tcgcgatcgg
     9601 gccgtgcagg aaatcggcgg ccgagaaacc ttccgctacc acgtagcacg tctccttaag
     9661 cttgagcgcg aactcaagcg cgttggcgta gttcagccca cgtcccacca cgaccgtgtc
     9721 ggtcatgaag cgatagcgct cgaccacttt ggcgacgtcc ggttccgcat tcagtgccgc
     9781 catgacccag tccggtatcc ggtgcagttc ctccagatcg atgggcgctc caagagcata
     9841 tgccagcagg tagaacatca gcacctggcc agtgtaggtt ttggtagccg ccacgctctt
     9901 ctccttccct gcacggacga ggaagacatc tttggagagc tgggcgagag tactctcgga
     9961 ttcgttggtt atgcttgccg tgaaggcgcc ctgatcgacg gcgcgctcca gtacgaggtt
    10021 tatgtcagtg gactctccgg attgcgacac cgcaacaacc agcgcatcct tcaggtcgac
    10081 cgacgcgccg tacatcgtgt agatcgacgg cgcggaaagc gacaccggga tgccggtagt
    10141 gatctcaagc aggtagcggc cgagttgcgc ggcgttgtcc gacgtgccgc gagccgtcag
    10201 gataatgaaa cgggggcgcc ggtcaccgag gcgtttgcgg aagcggcgcg ccgcctcgag
    10261 cccgctcttc aaggtccgtt ccaaggcatc cggctgttgt cgaatctcgt cgagcatctt
    10321 ggacatcggt atcaggatac cagaggggtc cgctggcggc atgtcaccca cccactggaa
    10381 ggtggatgtg tcagcatggt tcagagcgga gccggcggtc ggttttccga acctgtccaa
    10441 accgcctgcg tgggcagttg gtccggtgga agactcagca gcgcgggttt gagcaccagc
    10501 aggatccgcc gctcatcgtt gttgtgggtt tgttcgcgga agaggtggcc cagaccggga
    10561 acctgggaca gaccggccgg tccggtgacg cttcgtgtat cctgcgcgac gagcatgccg
    10621 gccacaacgg cccactcgcc gttccgtagc cggatctgcg acttcatttg ccggtttgcg
    10681 atgacgggga tgtcgttgaa gctctcgccg gtcagcacct tgaattcgct ctcaagatcg
    10741 agtgtgactt cctccatccc gtggactttc ggggtgactt tcagcatcag cccaaggtcc
    10801 tcaaagttaa actggggcgg aaatccagaa gtctggccct cggtgaagcc caggaacccg
    10861 ccggtcacaa tggggtactt gtcgccgaca tgaatggacg ccgcctggcc gtcggtggag
    10921 cgaatttctg tgcggagcag gacgcggccg agcgaacggc tcatccgcgc gaacatggcc
    10981 gcgtccgtca ccccaattcc gaacagcgtc ttgccgccgc cgaacgtaag aaacttcgtg
    11041 aagcccgacg gtattgatgg cgtggaatga aaccagtcac cgaaatagac gagaggaaac
    11101 atggtctgga gcgacaggcc gtagctcgtc tcctccgccc gggtcagttc gagaaactcg
    11161 acttcgatcg atacaaccgc tgggtaacgc atcaactggc ggaacaactg ctgagcgggc
    11221 cgcaccctgg aaacgcgatc acgaaagagc accaaccgcc gctgcgtatc gacggtcagc
    11281 cgcgtgatcg ccagagcctg ctggacggcg cgggcggcct cctgtgcctc ctgaattgtc
    11341 acgggggccg cgagttcttc catcacagtg acggtcggct cgagatcggc ccgtttctgc
    11401 gtgttgtcct tggccaccag gaatacacgt tcggagacag gaactacgaa ggaagcggtc
    11461 gccgcctgga gggcgttcag cgcttcgcga tagtccgcgt cgtccaaccg gaaccggaac
    11521 ggcgcaaccg gctgatagtc tccatcaaac accgcgccca agccatacgc cgcggccacc
    11581 tgctcgaata ggaaccggga gtcccctcga aaatcgaggt ccttccgatc cgttgacgcc
    11641 ttcagttcca tgggggcttg cggttgtctc gcttcagcta gttcgtcatc ggtaatccta
    11701 gctggtacac cattttccag aacctgcttc ccggcgggtg acagggcggt gtcggatttg
    11761 ggaagtgact tagctaccgt aacggcccgg cttcgcaacg tctggctgcg aagccagtat
    11821 ttcggttccg ccggattctt tgcagccgct tgagaataaa gaagataggc acggacgacc
    11881 tcgccggagc gctcggcgtg ctgcccctcc ctgtagagtt gctcggcgct cttttcagct
    11941 ccgggaaggg aggtcgcggt gacaaacaca acagccgccg cgagaagaaa cctcacgaaa
    12001 gaattgacgt gcagccaaca gaaagcgtgc aagtactgtc taaattcgtc ggttcgtact
    12061 ggtctggcta tggataattc ccacgcattt cctgacccaa actcataaga tctgatgtag
    12121 attgtcatta tgagtatttc gactcccacg gcgcaacggc catccttccg cccgtccgtg
    12181 cctgacggtc tggagaaact ggggattcca gacgttttgg tacaggacat cgtgctgcgc
    12241 cgcgtcatga tcgagcggac cagtactctt cagtccctga gcaggtcgct aaagctcgcg
    12301 ccacccgtgg tggaagcggt tttccggcaa atgcgtcacc gccagcttgt ggaagtactg
    12361 gggatggtcg gaaacgacta tacgttcatg ctctccggtc ccggccgcca gctcgcgacc
    12421 gagcgcttcc aaatgtcgca gtacgccggg ccgtgccccg tgtccctgaa cgagtacctc
    12481 gcgggtgtcc gctcccagtc cgcccaagtc gaagtcaacc gcgataagct ccggcgcgcg
    12541 ttttcggacc tggtgctgac ggatgttttg ctcgatcagc ttgggccgtc ggtcatttcg
    12601 caagcagcgt tgtttctcta cggctcgacc ggaaacggca aaacctctct tgccgaacgg
    12661 cttatccgcc tttacgaaga cgacgtcgtg ctgccgtacg cagtcgaggt ggacaaccag
    12721 atcatcattc tctacgaccc ggtcgtccac caccgtattc ccgacaatga agatgagatg
    12781 gattacgacc cgcgttgggt tcgttgccag cggccgtgca tcattaccgg cggcgaactc
    12841 gtaccgagca tgctggacct gcgcatggac gaggcgtcgg gcacctacgc agctccactt
    12901 cagatgaagg cgaataatgg catcctggtg attgacgact ttggccgcca gttgattccg
    12961 ccacgcgatc tgctgaaccg ctggatcgtg ccgctcgacc gccgtgtgga ctacctcatg
    13021 ctccgctacg gtgtaaagtt ccagattccg ttcgagttga tggtggtgtt cgcaaccaac
    13081 ctggagccgt cgagcctggc cgacgaagcg ttcctgcgtc gaatccagaa caagatcctg
    13141 gtggaagagg tctccgccgg ggtgttcgac gagatcctgc ggcggtatgc catcaccaaa
    13201 ggcattccgt tcgagcagga caaggcggag tacttgcgtt cgctgtgcct gcgcgacgga
    13261 cgcaccgttc tgcgcgcctg ctacccgggc gacatctgcc gcatccttgt ctcgatcagt
    13321 ctgtatgaag ggcgggcggc ccagatgacc cgctccgacc ttgaacgcgc ggtcaacctc
    13381 tacttcgcgc gggcgtaggc ctggggccgt ccagcttgcg acaggccggc tacgccctgg
    13441 tggctcagtg ggtggaaccg gggggaacgt aatcgggcgg gaacgctgcg ccctcgccga
    13501 agaagtattc ctccatttgt ttgagaagga actcctgcgc ttcgcgagta ccaaggttca
    13561 ggcggtactc gttcatgatc atcttcatgt gctcgaccca cagcctccac gcctctttcg
    13621 aaacgttctc atagatgcgc tggccgagcg gatgattgtc gaagggcggc tccgcgagcc
    13681 cctccatctc acgctggaac ttcacgcaga agatctttct cgcgcccgag cgcgcttcac
    13741 cgcccaccgg gggcacgcta ccgctgccca ttcgtcgctc ctcctggaat gataaggctt
    13801 cgatctcagg atagcgcagg cggaccggcg gcgcgggaat cagcgcttct tttcgaggaa
    13861 ttcggagagc ttcatcagtt ggccggtacc cttctcgagc tggcgctgta cggctctctg
    13921 gtaccggcgc agagtgttct ctcgcgccga ctcgcggatc tggcagttct caatgtcgag
    13981 caggatttcg atctcccagg gccggaacgt attgcgcaca atcttccccc gcagcagttc
    14041 tcgaatgagc cggttgaact gggcaagtaa ctcttcggag ctggccggct gttcatccat
    14101 cgcttccata actatatcgt gctgtaccgg ccagaccatc ctacggccag tcccatgagt
    14161 actgatccgg ttatagtact ccagatggga ggaactgtcc actctctctg atcaacagaa
    14221 accgttataa tccagagcta tgcgcatcgt cgtcaccttg gtcttcgctt ccacgcttgc
    14281 ggcgcagatt cccgcaacgg atctgcgaaa cacatacaca ccgaatacgg acacgcactt
    14341 cgcgatgccg gagtacaaaa ccctggcgga ttgggaagcg cgccgcgggg agttgagaag
    14401 gcaaattctc ctcgccgccg gtctgctgcc gctgccggag aagacgccgc ttgatccaca
    14461 gatcttcgga cggctcggac gcgatggcta cactatcgaa aaggtcctgc tcgaaacgat
    14521 gccgggctac tacctcgccg ggaacctcta tcgccctcgc ggccgaacgg gcaaattacc
    14581 ggcggtgctg aagccgcacg gtcactggaa ctatggccgc ctggaaaacc agccgttgtt
    14641 ctcgggccag gcgatcgcgg tcaatttcgc gctgcagggc tacgtggtgt tcgcgtatga
    14701 catggtcggg tggaacgaca ccattcagac accgcacgcc tttgggagcc cgcgcgagca
    14761 attgtggtcg ttcggaccgc tgggattaca attgtggaac tcgatccgcg cggtcgattt
    14821 tctggaatcg ctccctgacg ttgacgcgaa acgcatcggt gtgacgggcg cctccggagg
    14881 cggcactcaa acgttccttc tgacggcggt ggacgatcgc ctcgctttct ccgcgccggt
    14941 gaacatgatc tcggcgatca tgcagggcgg ctcgccgtgc gagaacgccc cggggttgcg
    15001 cgtcggggcg tttaacgtcg agatcgcctc gataatggcg ccacggccga tgatggtcgt
    15061 ctcggctacc ggagactgga cgcgcaacgt tccgaaagag gagttcccag ccatccgccg
    15121 gatctacgaa ctgtacggca agacagggaa tacggacgtc atccagatcg acgcgcctca
    15181 taactacaac caggccagcc gcgaagcggt ctatcgcttt ttcggcaagc acgtactcgg
    15241 cgcgcaggaa gacagcagct tcgccgagaa agaagttcgt gaagagaagc tgcaagacct
    15301 gatggtgctt cacaaccgca ccttgcctgc gggcgcactg agcttcgggc agatcttcga
    15361 acaatggcag gcgatgtcgc gcaggcaggc cgagggcgtc agggaccgcg ataccattcg
    15421 ggaacggctg cggttgacga tcggcgtcca gtggccgaaa gaggtcctgg acagcgtgca
    15481 aggtgagcag gtggtgttga gccgcgccgg caaaggcgat cgtgtgccgg gtatctggat
    15541 ggatggccgt gcgccggccg ctctcgtcgt acatccggac ggcgccgacg ccgcccgcaa
    15601 ctcccgggaa gttcgcgaac tgaaagcatc cggacgggca atacttctga tcgacgcctt
    15661 tcagaccggt tcggccgttg cgccccgaga caggtcgcat cgctttttcc taaccttcaa
    15721 ccggagcgat gacgcgaacc gggtgcagga catcttgacc gcgttggcgt tcctgaagtc
    15781 gaagaaaccc ggcgagattg aactcgtagg gcttgacaag gcagcaatct ggagcgtttt
    15841 cgcggctgct ttggcccccg aaaaggtgaa gctcaccgcc gcgacggatt cgttcagcgg
    15901 cacagaccag gagttcctcg accgtttctt cgtgcctggc atccaacgcg caggcggctg
    15961 gaaggcagcg ctggacctcg tggccgcgcg ttgaacgtgg ttcgtaatca gccgccgagt
    16021 ttcttcagac ggccctcgac gtccgccttc agcttttcga tgaactgcgt ggtgtagccg
    16081 gccacagaga gcgaggcgtc gttgaccagt ggagtcaggt ctaaggcgaa cgtgagcgct
    16141 gtttccacgt cgacgccgtg cgaggcgaaa tacgtcgcgt tggcaagccg gcggccggcc
    16201 gtcgccaggt cgtagcgttt gccaccggtc acctgtgagt cgaacacacc gacgagcgct
    16261 gcagcgacgt ttgaacgtgc ggagaccggc agcgattgct tgtcctcgtc gggcagccag
    16321 tcgaggtctc gccagacctc gcagccgtag accttctccg gacgctctgc gggagggagc
    16381 gagcgcagag cgtcgatcgc gcgtagcata gttcccacgt gcgtgtcgtg tttgtcggcc
    16441 gggttgtgca ggtagacgac tttcggcttc gctgctttga ggattgcgag caggtcgtcg
    16501 cgcaccgcgg cgaccttcgg gtccttcact tcggaactag tgtagtccag ttgaatctgg
    16561 caggcgtaat cgccgacaaa agcggccttc tgttgctcac gccgccgaac cttctgcatc
    16621 tcttcatctg tgtacttctc atagatgccc gcgcgggggc tgcccgcgcc gttggtcacc
    16681 accacgccgg caaaccactt gtcgtcctgg ccgaagcact cggcgatgcc gtggtacgcc
    16741 atgatctcga tgtcgtcttg gtgggcgcag atggcgatgt gcgttgtgcg cgcaagcgcc
    16801 gctgcctcac tgattccgtc gggtacgtaa aagtccgcgt tgggcttgta gaatttcatg
    16861 ttcgcttctc catgtgtacc tcagccgctc acttcctgac ctggggcagg ctggcggcgg
    16921 ccaccgcctg gccatgccgc ttttctttct cgcctggggt gtgaacttgg attgattctt
    16981 cgagctcggg gaactccgct ttcagaacct tgtgagccgt cgcgaggatc agatcaccgc
    17041 cctcgcccga catgaccctg ccgagcacca acaggtgccg gaggtcataa aacgatgcat
    17101 agtgggcgat ggcgtagccg aaatagacgc cgatcgactc gaagattccg cgcgcgcggg
    17161 cgtcgccccc tgccatcagt ttctgaaccg tgttgagccg ttccgccaac ggcatgtccg
    17221 cggagacttc gatgccggcg gctggaatca gcctcgccac cgcctgctgc gagaaatact
    17281 gcgcgccgac gccgtagtct ttcgaccatt cgtccatcgg cccattcggg cggtaatcaa
    17341 cgggaacaaa cgcaagctcg ttcagccaat tggtgatatt gccctgcggt gtgacgtagc
    17401 cgcctgcctg gctggttccc atcgagacgc cgagcacgcc gttgtcgttg agcgccatcg
    17461 agccggccag agccgttacc tcgccgtcgt tcaccacctc gaacggaatg ccgccccacg
    17521 cgtccttcag ttcgtagaaa agccgccgca cccggctgtc gaacagatcc ggaggcacgc
    17581 cgcggaacag cgatgcgacg cgcacttcgt tgttcacgta cacgccggcg gcgcttccgc
    17641 cgatggcgtc cacgcgcggc agatgggagg cggcgcgctt cagcgtgtcg ttgacaccat
    17701 cgaaatgata ctgcgggtct ctctggaaat agggatccca gacgacctcc tcgctgaaca
    17761 ccacttcgcc gtcgatcacc gcggcatatt ttcggtcgct gccgccgaga tcgaacccga
    17821 tgcggcagcc gtcaagatgc cggccgagcg ccgccgtggt ttcgtgctcg gcgggcgcgg
    17881 cctcgtagct tgtttcctcg atcaccatcg gcgtgccgta gaccttctct cccatgaagt
    17941 ggaagtcgaa ggactgcttg cccgacggag agtaaacctc gcggatgtaa gcggcaatgc
    18001 gcgggtcgcc cgcgatggtg accttcgagc cgcctcgctg ccagagcagg aacttcacca
    18061 ggcgttccac gtagacttcg ttcgctgcca cgaacgcgcc ctcgtggggg aataccgtag
    18121 tgcgaaatac cgacacagag ccgtcagtgc gcgcgagcgc aatcgccagt ggtttcccct
    18181 gcccgctctc cgcgactttc gtctggtagg cccggcacca cagcgaagcg ggaacgaaac
    18241 ccgggtcgag aggaggcttt atttttgggg agatctggat ggtagaagac atagaaattt
    18301 ctctccggta tcaggacatc aagataagag aatagcgaaa caggggggct gcggtctcac
    18361 ctcgaggccg ctctgtgctt ctgccaccag atctccgggc gcttgtcggg accgaggtgc
    18421 ggattcggtt ccaccaggag gccatacagc tccggccgtc gtgcccggat gtagctttgc
    18481 ccaggcgcca ccgccagctt ggccgggtcg agtgtcgcca cgaccacctc gtcggccagc
    18541 cggcggcact ctgccagagt ctcgccgaag cagtccagaa tcatggcgcc acctggcttg
    18601 atggtgtctc cttccacgcc gatcgggttg ctgtacacgg cgaagacgcc gctctcgaag
    18661 gcgcgcgccg gaagccatcg catgagccag ccacggccct tcggtccgtc gaattcctgc
    18721 ctgcaccgta ccggatcatg ttcccggttg cgccagatct ccgggtccac cgtgccgcgc
    18781 ccgggcatcg gcgagggcag gcaaccggtg acgtgcggca tgaggatgat ctcggccccc
    18841 atcatggcgg tgatgcgcac gttctcggga aggttgttgt cgtagcacgt aagcacgccg
    18901 aacttgcagc cgagcagttc aaagactgtg tagcgttcgc cgcaggtcag gtgcgggctg
    18961 acgaaagcgt ggatcttgcg atgcttcgcc acgaagccgt ccggcgagac aacaacatag
    19021 cagttgtaaa gccggtcccc gtcccgttcc accaatccgg cggctaaaca gcagccgagc
    19081 tcgcgcgaca tctcgatcaa ccggcccgtg gacggtccgc cgggcacagg ctcggccaac
    19141 gccgtgatct cctccagcga cagattctcc aggaatgtgt agccggagat acaacactcg
    19201 tgaaacgaaa cgagttccac gccctcacgc gccgcctgcc cggctagttt cccgatgacg
    19261 ccgaggttgt agtctttgtc gccgtctttg gcttcgaact gcgccgtgcc gactctgatt
    19321 tcacgcatgg cagacctaca ttttatggtt tccatccgtg cggtgctggt ccgttcctgc
    19381 gcagatccga atcgaacgga cttaggatgg tatgattggg ccgctggaat cctgcatgac
    19441 cgcccgctcg cgtatcgcca cagtatcgtg gtgtctgttc gactttgcga actcctccta
    19501 cacgacactc atcatcacgg tcgcttacgc tgtctacttt cgcgacgtgg tggtgaacgc
    19561 gaaggacaac cgcggcgatc aactgtgggg cattgccaac ttcctggcga tgctggtgat
    19621 cgcactgacc tctccggttg tgggggcgtt ggccgactgg tcgggctgga aaaaacgctt
    19681 tctgattctc agtacgctgc tggcggttgc ggcgacggca ctgatgttct tcgtcgggcc
    19741 cggcgacatc gcgtccggca tgctgttgtt tgtgctcggg actatcggtt tcgaaatcgg
    19801 ctacgtgttc tacaacgcgt ttcttccgga catctccact ccgaggacga tcggacgcat
    19861 ttccggctgg ggttgggccg tcggctatgc gggcgggttg ctatgtctgc tcgcctgccg
    19921 gccgtgggtc gcgcagccgc tccgggatgc ggccggtgcg atcagcacag ccggcgtcga
    19981 ggcttaccag acgtcgttcc tcatcgtcgc cggcttttat cttctgtttg cgctgccggc
    20041 gtttacctgg ctccgcgagt ctgagccgca gggccggggt gtgagttgga tggagagcgc
    20101 agctgtcggc ttccgtcgcg tcggagaaac actgtcccac ttgaggcgct accgggagac
    20161 cgcgaagtac attctcgcgt cgcttttctt caccgacggc atcaccaccg tcgtcagctt
    20221 cgccgccatc tacgccacca ccaccatcgg cttcaccaat gaggaaatgg tcttcctgtt
    20281 cctggtgatg aacgtggttg cgttccccgg cgcgctcgtg gcgggatacc tcgccgactt
    20341 cctcggcgcc aggcgcacca tcatcctgac gctcttcctg tggatcggtg tcgttttgct
    20401 cggcgcttcc gcggcaacga aaacggtctt ctgggtaatg gccttcctcg ccgccctggg
    20461 aatgggaagc acgcaggcag tcgggcgatc attcatggcg cagatttcgc ctccttcgcg
    20521 ggagtcggag ttcttcggct tctatgtact ctccgggaaa ttcgcatcga tgttcgggcc
    20581 gctggttttc ggctcgatct cgaggttcac tggaagccaa cgggcggcgg tgctgtccct
    20641 gttgccgttc tttctgctcg gactcgccct catgtactcg atcaacgaag gacgcgcgcg
    20701 tgaggccgcc ctcctggaag ggaaaaccgc cgcgccgtga tacattgaca cgcggacttc
    20761 tatgaccctg gactttttcg agcggttccg ggaaaacctg gagcggttcc ctggtgttgt
    20821 cgctctccag acgctctccg acgccggccg ggagacgttt acctaccagc aggcggctgc
    20881 ggaaacgagg cggctcagcc gctaccttca agcgaagggc ctccgcccgg gagacgccgc
    20941 cggcatcctc atggaaaacg acgcccggtg gggactggcg ttccttgccc ttcaaagcgc
    21001 cggagccgtg gtagtgccgc tcgacgttct gcatcagccg caaaccctcg ccgggctcat
    21061 cgcacatgcc gaatgcaggt tcctgctcat agcgccgcgc ttcgtgccgc tcctcgaaca
    21121 gattcagggg cttctgcccg agccgctgcc cgttctggtt tgtggggagc cggcgggtag
    21181 cttcccttca tggtccgagg cgccacccgc gggggacgag acaccgatcc cgctcatcgc
    21241 ccgggacctc gacgatccac tcgttatcct ctacactagc ggcaccaccg gcaatcctaa
    21301 gggtgtggtg ctgacgggcc gcaacatcta tcgcaatgtg gccgaggccc ttcaggtgat
    21361 cgggccgaca caccgcgatc acttgctttc agtcctgccg ctctaccaca tcctcgcgct
    21421 ggccattaat ctggtgattc cgttgtatct cggcgcgcgc gttacttttc ttggcgcgct
    21481 ggaggcgcag aaagtcttca aggcactgaa tgaggaaggc atcaccatct tcatttgcgt
    21541 gccgcagttt ttctacctcg tgcaccgcag gatcttccag gaagtggaac gccagaggcg
    21601 ggtgaagcga ttcctgtttc gccgcctgct cgcgttctcg cgtttcgcca acctccattt
    21661 gggatggaac cccggccggt ttttcttccg gcaaatccat gagaagttcg gtccgtcgtt
    21721 gtggctgttt ggcgtcggtg gggcccgctt cgacgtcgag gtggctggcg attttcgcga
    21781 tctcggcttc aagctggctc aggcatatgg catgagcgaa actgcggcgc tgatcaccgt
    21841 cgcgcccccg gtggccgagg ctgtcggttc cgtcggaaag ccgcttccgc acgtggagat
    21901 tcgcatcgac cagccggacg agaacggcgt cggcgaggtt ttgacccgtg gagagaatgt
    21961 gatgctgggt tactggaaaa acaccgaggc caccgccgag gccattcgcg acggctggct
    22021 ttacacaggc gacctcggct atatcgactc ccacggatgg ctgtacatca ccggccggaa
    22081 gaaggacgtc atcgtgctta gctcgggcaa gaacatctac cccgaggaga tcgagtactt
    22141 ctaccagtca gattgcccgc tcatcaagga gatgtgcgtg ctcggcgtgc ccgatcccgg
    22201 agccggcgaa gaacaggagg tcctccacgc agtcatcgtg cccgatttcg acgcgctccg
    22261 cgaccgccag gtggtgaacg tgttcgaggc tcttcgctac gagatcgaga gcctgtcgca
    22321 acgggtaccg ccctacaaac gtgttcgcag tctggaaatc cgcacggagg ccctaccccg
    22381 cacgaccacc cgcaagctgc gccgattcca ggttcagcag gagctgggcc aacggacggc
    22441 ggccgcttca gcgccggccg tgacgccgga gccggagccg gacgacccgg tcgagcgcag
    22501 agtgcgtgat ctgatccgcc gcatgaagga tgcgccttcc atccgcggcg cgatgaacct
    22561 cgagcttgat ctgggtttca cctcgctcga gcgggccgag ttgatgttcg gcgttcagga
    22621 aacgttcggt ttgcgaatcc ccgacgaaga ggctaccggg gtcttcaccg tcgacgatct
    22681 cgtcggtgca gtgacgcggc gcctttcggg cgagctccgc gaagacggcc aggctcgcgc
    22741 ttcctggagc gacctgcttc gcgcccccct cacccccgac gaggagcgga tgctccgtgc
    22801 gcaactggcg cgccggccgg tggtggaagt tgtctactac ctcctcgcgc ggatcgcccg
    22861 cgtcgtggtc cgagtccttc tccgattccg cgtccgcggc gtcgagcgtc tgccgaacca
    22921 gtatccgttt ctcatctgcc ccaaccacct cagcttcatc gacgccttcc tggtgatgag
    22981 tacgcttccg tttcgcgtgt tgcggcggct gtgtattctc ggtgacagca ggtacttcag
    23041 cagcggcgtc acggcccttg tcgggcgcct tggtaaggtg atccccgtga atcccgaccg
    23101 cggaccacaa gtgtccctgc gcctggccgc cgagggactt cgccgcggcc tggtgctgtg
    23161 cgtgtttccg gagggcgagc gttcgatcga cggcaagctg aagccgttcc gtccaggcgc
    23221 ctccattctc gcggcggagt tcggcgtccc ggtggtcccc gccgggattc gaggcgcttg
    23281 ggaggtctgg gcccgcggat ctggccggat acgacttcat cccgtctcgc tcgaattcgg
    23341 ccagccgctc cagccgcccg ctccaggaac gccctacgag accgtcaccc gcctgctcca
    23401 ggaggaggtc cgccggctgg tcgaatgacc tctacccgat ccgaccactc tggccgttgt
    23461 ccaatgtttc gaccgccgag ttacgcacca gcggcagccg gatgaggaaa cgcgcgcccc
    23521 ggcctggttc agattccacc cacaggtcgc ctccctggtc cagcaccgtc tgtcgtgaga
    23581 gcgccagtcc gagcccaagc ccgttcttct tgccgaatgt gacgaaaggt tggaataact
    23641 ggtcccggat ctgaggcgag atgccgggcc cggtatcccg tacctcgacg agtgccactt
    23701 cgccgttgcg tgatgctgac accgtaatcg tgcctccgcc cggcatcgct tcgagcgcgt
    23761 tggagaacag gttgaggaac acccgttcca tccggccgcg ctcaagcggg agctctagct
    23821 cgccgggcag gtcaattgcc agcgagaccg cctgtctctc ggcgaccgcc cgcacctggt
    23881 cggcagcgtc cacgatgacc tcccgcagca cacacatctc cgccctcccg ctctttccgc
    23941 gggtaacgtt gacgaggtcc tgaagcagtt cctgaatgcg ccgggacgcc ttgtagacgc
    24001 tgctggcgac ccgcttcacc tgggggggcg cgagttcggt gtccaccagc atctccgcgc
    24061 cgccgtaaat cgccgccagc ggattgcgca ggtcgtggac gatggaaccc gccagcctgc
    24121 cgatggtcga aatccgctct cgccggatca gttcctgccg ggcggactcg agcgactggc
    24181 acattttgtt aaacgtcgcg gcgaggcggc ccaactcgtc ttcgccctcg ctctccacgc
    24241 cgatccggta gctgtaattc tcgcgggcca gttcagccgc cgcctcgtcc agcttcttca
    24301 ccggctccac aatcttccgc gcgaacaggt gggtcagcac aagacccgcc accaccgcca
    24361 gcagccagat tagaataacg tcgcggcgaa gagccgccac tcgctgcctg gcgccttcga
    24421 acgaccgcag aatgtacagt gcaccgatcg gtttcccgtg cacgtcgatc agatcgcgtc
    24481 gcacgggcat gtattcgtag acaccgtcac tgaccctgga tcccggcgtg tggaccgcca
    24541 ccgccttccc gagttcctgt gaagcgcggt cgttcagcgt tgaggcaaag acacggccct
    24601 gcgaaaggaa gacaaactcg ctgcctcctg tcgccgcttt cagccgctgt gccactgggt
    24661 ggttcaccgc gtagccggtg accagcacgc tgatcagcgc cggaccgcgg ccggagtcga
    24721 cataaaccgg agtcagtacg agctgtacca attcgttgtt ctccaggaag aaccccgaaa
    24781 cctgttcggg gaacttggat cgagcgtctg gtactaccgg ccatgaacgt ggagcggcgg
    24841 acgctccttc gcccagggat gcgatggtgt tccctttcgg atcggtcacc acgaagaagg
    24901 ccgtctcccg cagatcctcc gagacgcgcg cccacacctc ctgcgccgcg tcgcgcatcg
    24961 tcgcttggtc gcgcgtgccg aacgccgcgc gaacattggg gaggctgctg atgatcgacg
    25021 ccaccgaccg cagcatctcc gcgcgcgccc tccacagcga agcgtacgcc tggaatcctg
    25081 cctgcacctc ctcctcgaga ttgcgtgcgc tggtttcgag cacgtgccgt tgcaccaccc
    25141 acccggtgac cgcgaacaac gctgtcagcg ccaccgaagt ggagagccaa atcttccaaa
    25201 gcaacgagag ccggcggaag ttcatcggcg cactcccggg tagaccatgt ggtccggcgg
    25261 cggctcgggg tagtcccggc cgtacttgtt cttgtgcggt acctcgaggt atccggactc
    25321 ggacacacga atcagcggaa gcccgatcgt ctcccctccc accgtgatct tccgctccag
    25381 cgcccgcagc gactcctcgg ttgcccgctc atgaaacacg cgtacctggt aatccccggc
    25441 cggcacgcct tcgatctgga aatcgccgcg gcggtccgtc accgccgcgt acggcgtctt
    25501 cgtcaccacg atcaccgcac tcatcgtcga gtgaatgttg caaaagacac gcacaacccc
    25561 gtctcgccgg aacgtcactt tctgtgtcat tccgggcggg taaaggccgg tgtcgaacgg
    25621 ctggccgctg aagtttgaaa atgcgttgtg gaaaatcggg tccaggttcg gaaactctac
    25681 tgtcgtaccc acaggaatcg caaggatgtg tggaacaaac cgcttgccct gctggagcat
    25741 ctttgcggtt cgaggctcag ttcgcggagg gtctccgtgg acgggatcga gccacacgac
    25801 gacgcccgag aagttgcgat gccgccgcac ctgcgggtca cgtgaatcga cgagctccac
    25861 gcggccgccc actgttgccg ccagcgcaca aaagctcaag ataaggctag aacaggtacg
    25921 ccagagccag gtcatagtgg ttgttgatcc gcgttccgac tccaagatag tccgttcgta
    25981 cctgcgatgc ctccaggctc acaatcacgt tcggcgaaag gcggtacatg acatttgccg
    26041 cgcctgcggt gttctttccc acctgccccg gaagcaggtc ccggttccgg tcgtcatggc
    26101 gtccgcccat caggttaaac gaaagccgtt gcgtggccaa cagcgagagc tgggcccagc
    26161 cgccgcggct gtgtaccgga atgaccaagt attcgctgat gacggtgaac ccctgccgtg
    26221 gcgagccgaa atggcccacg ttctggccgc tgaagaacgc ccccgtgaac tccaactttc
    26281 gccaggggtt ggcgaaccag tcgagcgaga agagattcga cgggatcgac gtgccggcga
    26341 cgtgcgaggt gctcgtgtga aaaccggggg ctatctccag gcgtctcgtg tggtcaagat
    26401 ctttcgagaa cagaaatcgg ccctcgaacc ctggccgccg tctcgcgacg gttggggcgt
    26461 agtcggatgg cacggccgtc gtctcctcgc ttgtctggag cactcccaac tgggcgctga
    26521 atcgggcgtg ctggcccagc cggaaccgct gctcgacccg gatctgcggt tgccaccgcc
    26581 agaggttccc cgcgccggtc aagggcgaga ttccaacttg ggccagagaa ttcggctcgc
    26641 gcggcgcgaa gatcggcttc tcctggccgg ccaggatgct ccgtgacctc cactccagct
    26701 cgatcgaacc cgtacggatt cgcgcctgct ggttgagcga gtcgccggtc ccgccgaaga
    26761 agtcagcgta gagcgagccg cgaaccttgc cgccccagac cgtggacgtg ccctcgtact
    26821 gcaaaccgac gacagactgc cgcaagctcg caccacccgc cgcgctgccg gaaatgcgtg
    26881 ccgccagcgg cgggttgtcc gcgccgccgg acgcgcggcc gttgaggaag gcgttgaaca
    26941 gggccatgcc ggtgacacgc agcggcaggc gttgcgacga ctccactttg gtctgcgcct
    27001 gttcgcggat gcgctcctcc tggatggcca gccgctcttc gacgccggac tgcggcccgc
    27061 cgtcggaaac cagccgccgc agctcgccga cctcctcgcg gagcgagcgg ttttcggatt
    27121 cgagccggtc cagtcgctcc aggaccgttt tccactcggc cggtgtctgc gccgctgcaa
    27181 ctccgggcag caacaggcag acggcgagga atagacgccc ttttcgcggg cgaaacgatt
    27241 tacggcgcga aagcattcag taactcctcg actttgttcc gcacctcggt gggagagtaa
    27301 ggtttcgcga agaacgcatc ggcgccaagc ctccggattc gacccggggt ctccggatcg
    27361 gtgtcgccgc tcagcacgat gaccggcaga cgctcgtgcc ggtcgccgga gcgcaaacgc
    27421 tcaatgagct cgaagccgtc catcagcggc atgtggagat cggtcaggat gacgcaggct
    27481 gtccccggct ctagagcttc cagttgctcc agcgccgcgc ctcccgtggg ggcgtgcgaa
    27541 accaccacgt cgggcaggct cgataacgcg atctccagcg ccgaggcgta gtatggcgtg
    27601 tcctcgacga tcagtacctt tcttggcatc ccctcgcctt ttccgagtat tacttaggta
    27661 actgcggaac ggatgttaaa tttatcgtag cagtgcggtc agggaatgaa tcggtagccg
    27721 acgccgtgga cagttaggaa gtgtttcggc acgctcgcat cgggctcgag tttctgccgg
    27781 agcttgacga cgtggacgtc cacggtgcgc gtgttcggga acgattcgta gccccagacg
    27841 gagttgagaa tcatgtcccg cgtcagggct cgatccggat tatgaaggaa atacgccagc
    27901 aggttgtatt cggccggagt catcttgacc tcctcgcctt tacgggtcac gatccgccgc
    27961 tcgacgtcca cctcaacgtc gctgaagtgg agcaccttgc gggcgtcggc gacagttcgc
    28021 cggagcacgg cgcgaattct tgccagcagt tcccgggttc caaacggctt gaccacgtag
    28081 tcgtcggcgc cgatctcgag gagaagtacc ttgtcgatct cgtcgccgac agcgctcatc
    28141 acgatgatcg gggtctggat cttggctgcc cgcaattgct tgcagatctc cgtgcctgtc
    28201 attaggggca ggcgcaggtc tacgaggatc atgtcgggct taagcgtcag agccttctcg
    28261 aagccgtcct tcccgtcggc cgcgggtacg ggacggaatc cttcctgctc gagcatcacc
    28321 gcaattgttt cgcggagatt ttcgtcgtcg tcgatgacga ggacagtctg catgaacccg
    28381 tttcgattgt agctgatcct ttcccgccac tcagaacccg cgcgctaccc atcggccggc
    28441 ctcgatctga aatcgaacca gtgttgtgtc cgattgcagg cggcgcacac ggagagcata
    28501 acgggaactt cgacaagcac ccccaccacc gttgccagcg cggcgcccga ccccggcccg
    28561 aacagcgcga tcgccgtagc cacggcgagt tcaaagaagt tgctggcccc gatcagcgct
    28621 ccgggcgcgg ctacggaata ttccacccgg aacagccgca tgagaccgta ggttagcgat
    28681 gagttgaagt agacctgaat aaggatcggg atggcgatca gcacaacgtg aaaggatttc
    28741 ccggtgatgt tgtcggcctg gaaggcgaag atgaagacca gggtggcgag cagggcggcg
    28801 atggtcaccg gtgcgaatcg cggcagcaga ctcgtctcaa accattcttt gccgtgcctg
    28861 cggatcagcc acgcccgcag cagggtgccc acggtgagcg ggatgacgat gaaggcgatg
    28921 accgagtaca gcaacaccag gaagggtacc gacagcgacg acgcgccggc gacaagaaac
    28981 cgcacgatcg gcgcgaacag caccagcatg atcaggtcat tcaccgatac ctgcaccagt
    29041 gtgtaggccg gatcgccgtc tgtcaggtag ctccaaacga agaccatggc ggtgcacggc
    29101 gcggcggcca ggatgatcac acccgcgatg tactggtccg cttctgccgg gctgatccag
    29161 cccgaaaaca cgacacgaaa gaaaatccag ccgatcagcg ccatggagaa aggcttaacc
    29221 agccagttga caaagagcgt caccagcagc ccccgagggc gccggcccac gttccggatg
    29281 gaagtgaagt cgactttcat catcatcggg atgatcatca gccagatcag gaccgcgatc
    29341 ggaacattga tctggcttcc cgacccgaat tccatccgcc gcaacgtgtc cacgagcccc
    29401 ggtgtcagct tgccgaggag cacgccagcc agcatgcaga ctcccaccca caacgtgaga
    29461 taccgctcga aaaggctcag tctcttctcg acagcctgag gttgggctat tgtctgttcc
    29521 atacaggcgc ctccaacacg aacttcttca ctgggccgcg cacaccggcc gctcgctcgc
    29581 cagttccctc agccgttctg gacccaaggg tgcttcaggc ttccatgaca ccaacgcaaa
    29641 tcggccagcc aagtgatccc tgacctccgc gataaacggc aactcgcgcg tacctcgatc
    29701 caccagcacg ggatcgcgga atccgtcctg gaagaagctc tggttgatca cccatgcaaa
    29761 cggcgtgatg ccggcccgga ggaggtcatc ctggagccgc gcggcttcat gaaccggtgt
    29821 cgcctcgggg agcgtgacga gcagaacgcg agtgaacgaa tcatcccgga gccgcggcaa
    29881 caagcggcgg acggtgtcag gcgtgtcact catggtccgg gacacctcgc ggtgatacga
    29941 ctccgcggca tccaacagca atagagtgtg gccggtcggc gccgtgtcga gaacgacaaa
    30001 ctcatcctct ccgcgggcca cggtttgtgc gaacgcgctg aaaacggcaa tttcctctgt
    30061 gcaaggcgac cggaggtcct cttcgaggag tgcgattccc tgctcgtcaa gattcggtgc
    30121 cgacttcgcc atcacctgct ccgagtacat gcgcgtctcc acagccggat cgatccggct
    30181 gacggaaaga cctcggactg ctgtgccgat ggtgtacata aggtgggccg cgggatcggt
    30241 ggtgctcagg tggacccgat gtccacggtg cgccaactca acggcgatcg cggacgccac
    30301 cgtggtcttg cccaccccgc cctttcccat ggtcatcacg acgccgtgac cggccttttc
    30361 gatctggtcc acgagatcag cgagcggagg agacggcgac gccgtgagcc cgctactgtg
    30421 ttccacagga gcgtatcctg ctccacccac agtgaagacc gcgcgtaggt tctgaatccc
    30481 catgggggca tagggcagca ggggaacctc aaaggtcggc agaaggcgaa gtccatcggg
    30541 ggtttttgcc agcgcctcgc gacccctctg ctcaagcgct cgggccgcag gatcagattg
    30601 gtcgcgcgca atgaagactc cattgagaaa caagtattgg ttgctgacgc cgatgtcggc
    30661 taattccgtc cgtgtccgtt ccgcctcggc cagcgccgag gtctccggac ggctgataag
    30721 cacaaacgtc gtggaagtag cgtcgctgag tgtcttcagg cttgcgtcgt atagcgcctt
    30781 ctgcgcctga aggccggcca gcggacccag gcacgatgtc cccgtcgtgt tcgtagcgat
    30841 gaaaccggtc cacgctgccg ggagtttcaa gagccgcagc gtatgcccgg tgggggccgt
    30901 gtcgaagatg atgtggtcga agccttgggt cgcgtcagga tctcctagca gcttggtgaa
    30961 ttcgtcgaaa gctgcgattt caacagtgca tgctcccgag agctgctctt ccatgctgtt
    31021 gatcgcagct tcgggcaaga tgtcgcgata tggacccacg acgcggtcgc gatacgcagc
    31081 cgctgccgct tccgggtcga tgttgagcgc gaacaggcca tcggctccag gcacggccac
    31141 aggtgctccc gatagtgtga ctcccagaac ctcatccagg ttcgaggccg ggtccgtgct
    31201 gacgagcaac actcgccttt cgcaccctgc cagcgcgacc gccgtggcac aggcaagtga
    31261 cgtcttacct acgccaccct tgccggtgaa gaacaggatt cgtgtggcag tttgaaggaa
    31321 ctccgcggta cgcacgccga atcccctcaa ccgcagcagc gtcgctggac gatccgcagg
    31381 ccggcggcgt ccggcttccg gatacccagg tcgaggccgg cagcgttggc gagttcctcg
    31441 cgactggggt agcggccaat gctgatcact tcgccgtcga gcagtacgag cgggaggcag
    31501 gtatttccat gctcgtgcag aagggctttc accacttcgt tgaaggcaaa ggcttggggt
    31561 tgctgcgcca ggttgtatcg ctccacgtga acgccctggc cggctatcca taggaagtcc
    31621 gagccaaact gtaccagcgc ggggtcgaca ttcggcccgc agacaccggt cgaacagcac
    31681 atcgggggat cgaagacctc cagctttttc atcgtgggta tgagttcctc tcttatcgtc
    31741 gttcggcgat agttatagga ttgcgaccag cgccttaaga cgccggagcg ccgggaaatt
    31801 gatgcagtac ccgacacgcg agccctcctc ccggctgcgt atcaagccgg cttccttcag
    31861 aattcgcagg tgttctgaga ctgtggattg ggccagtggc agctcgccga caatttgact
    31921 gcacacgcgg gcctccttgt gagacagaag gcggagaatt cgtacacggg ccggatgcgc
    31981 aaccgccttg gcgagcgtcg cgagttcctc gtccgccgcg gcgccttcca ccggccgcag
    32041 gtcggcttcc agttcgagtt ccggtgtgtc gcagcaggcg atggggtctt ttctcggagc
    32101 tttcatcgtt aatcgtcgat tgacgattcc aatatagcgc atcagccgtg tccggttcaa
    32161 gcgatatcag gaggtggtta tccttgtatt ccggtccggc cggcagtatc ctgcacatgg
    32221 ggaagtttgg gtcatgaagg ctcggcgctc catcgttgtt ctggtggccc tgctgggagc
    32281 caggctcttc gcagcggact tctgggagaa gaagaggttt cctgactgga gtgcgaagga
    32341 aatccggaag ctcctcaccg attctccttg ggcgcggccc gttcttgttc cgttggagac
    32401 gctgcgcgga cagcaagcca cctctatgcc ccacacgcag gcaaagggtg cctgccacca
    32461 atgctcggtc gggggcactc cggtgcagct ccaatccagc gacattccgg agttggccca
    32521 cccgctcacc gtgacggtcc gatggcacag tgccttaccc ctgaagcagg ccttggctaa
    32581 gttgctcttc ggtgacgagg tgagcacgtc tgcggctgcc gccgagttgc tctcccggga
    32641 ggacgactcc tatgtcctcg ggctttcgtc gctgccgcgc caggtgctgc ccgaggatct
    32701 tgagagcatc aagtccggcg ccgtactcag agtgcgaaac ggtccctcaa ttccggcgcg
    32761 ggaagcacgt gtaagagcgg aaggcgacat ggtcaacctg cttctgtact ttccgaaacc
    32821 gcagagagac gccgccggca ttactcttga gcatcgcgag gtcgagttgg gagtcaaatt
    32881 gttcgccatc gagatcaagc gcaaattcaa gctgtcggag atggtttacc agggcaagct
    32941 cgaactctag tgttttctgc aatctaggaa agcgatgctt ccgtgatttt ttctcttgac
    33001 atccttcgat aattccatta ttttagaaat atgaaaaagg tctccgggaa cgacgtgagc
    33061 cgttacgccg ccatgctggc ggccatgggg acggaacccc gccttcgaat catgcgcctg
    33121 ctgctgtccg ctcacccggg cggcatggtt gtaggggaca ttcagacgga gttgggcact
    33181 tccgcatcca ccttgtcgta ccacctcgag aaactgaaga acgagggact gattcgtgtg
    33241 cgccgccagg gcacgttcct ttggcacacg gccaacaccg atgcgctcca ggaattgctc
    33301 aacttcctgt acgccgaatg ctgtggccgg acacagacag tgaacatcga ggacctcgtt
    33361 tcgacgaaac ccctaaaaca agcggctctt gcgccgcgag aacccgaaaa ctgagcgaga
    33421 aaaacatcat ggatatcaag caagcagtga aagagagata cggaaaagcc gccttgcagg
    33481 tgactggcgg cggcgcgccc tgctgcggtg ggaattcctc cggtgcggga tgcggtgacc
    33541 cgattacgtc gaacctctac gacgaaaagc agacaggaga gctgccggag acggctgtgt
    33601 tggcgtccct cggatgcggc aatccgacgg cgctcgccca attgaacccc ggcgagacgg
    33661 tcctcgacct cggctccggg ggcgggatcg atgtgctgct ttcggcgaag cgcgtcggac
    33721 cggccggaaa agcttacggt ctcgacatga ccgacgagat gctcgctctt gcccgggaaa
    33781 accagcgcaa ggccggtgtc acgaatgtcg agtttctcaa aggggagatc gagaacatcc
    33841 ccctgccgga cgcgagcgtc gacgtgatca tttcgaattg tgtcatcaat ctctcggcgg
    33901 acaaggaccg cgtcctgcga gaggcgtttc gagtgctgaa gccgggaggc cgtttcgccg
    33961 tgtcggacgt ggtgatgcgc ggggaggtgc cgtccgcggt gcgccgcaac atcgagctgt
    34021 gggcgggctg tatcgccgga gccctcgagg agaacgagta ccgggcgaaa ctgaccgccg
    34081 ccggtttcgg cgacgtcgcc gtcgaaccga cgcgcgttta ccgggttgat gacgcccggg
    34141 agtttctcac ggcgtcggga atcgacgtgg aagcagcagc cgccgccgtg gacggcaaat
    34201 tcatgagcgc attcgtccgc gctgtgaagc cggcggaggc aggccgcgca acggcgcaat
    34261 gagtacggcg agagtgctga ttctgtgtac tggcaattcc gcccgaagcc agatggcgga
    34321 gggtctcctt cgtcatctgg ccggcggccg ctatgaggtg tttagcgccg ggacacaccc
    34381 gagtttcgtc cgcaccgagg cgattgccgc gatgcacgaa ctggggatcg acatatcggg
    34441 ccatcggtca aagtccgtcg acgagtttcg attgcaagcc ttcgactacg tgattaccgt
    34501 ctgcgacaac gccagggaaa gctgcccggt attccccgga aacgcgcgac ggattcactg
    34561 gagcttcgag gatccggcgg cggccgtcgg tgaccaggca gtgaggttgg aagcgttccg
    34621 gcgaattcgc gaccagattg cggatcgcct ccgcgacttc gtgcaggcgg ccgcctgacg
    34681 aatactcacg tgacgtgcaa cgttctccgg catgttgaca tctaaagaga agataatgaa
    34741 acggaggatc tatctgctga tgtcaaaact tcttctgact gctctgctgg ccacgaccgc
    34801 actcttcggt caatataagc tcgagtccac cggagcgccg cccgacgaat tggcgccagc
    34861 cgtcaaggaa gctttacaag cgagcggagc gaaaatcgtt ggagccgacg gatccgcgct
    34921 ctgtgagatc tggttccgca agtcgctccc gtctggtccc gcctctaccg agcaaggggt
    34981 gagtctgaag accgtcccgc acggagcaat gctcggtgtc atccgctttc ccaagcgcgg
    35041 gcaggaccgc cgcggcttga cgattcccga gggcgtctac acacttcgct acagcctctt
    35101 tccgccggac ggcaaccatc agggagtcgc cgcacagcgg gacttcctgc tgcttgtccc
    35161 tgctgccgcg gatactgatg tcaactcgac gccttcgttc aaggaagtcg tcgcgatgag
    35221 cgccaagacg accggtggtt cgcatcccgc aagcctgagc ctgtggaaag acgaagccgg
    35281 acacgagccg ggtctggccc aggaaggtga atccgactgg gtgctgcaca ccaagattgg
    35341 tgacacagcg gtcggtctga ttgtggtcgg ggttttccag ggctagggga tcttggccgg
    35401 ggacgagcct cggttcgttc ccggcgctac gtcgtcgaca gcgccgcccg catctcgttg
    35461 agggctttcg tcaaactccc cacctgctcg ccaaccggca agtactccat cgtgatgtac
    35521 ccgccatacc cggccttctg gatcgattta tagatatttg cgtagtttag ctcgcctgtt
    35581 ccgggatcat tacgcccggg attgtccgcg acgtgataga ccgcgacgta gggcgccgct
    35641 tcgacggctg tgcggatgac gttccccact tgcacctgct cgtgatagat gtcgaacagc
    35701 agcctgacgt tttggttgtc gacctcgcga acgagcttca gcccttcggc gcaggtggtc
    35761 aggaagaagc ccttgtggtt gaccttcgcg ttgagtggtt cgacgatcag cgtgacgttc
    35821 gccttcgccg ccagttctcc cgcgcgccgg gtcccttcga gcaaactggc gtactgttcc
    35881 tccctcgaac ggcccgggat ctcgtccccg ctcatgagaa taatctgagg gatttcaagc
    35941 ttctgcgcga aagtgatggc ttgcctgacg tcattgagaa aattctcacg ctgtgccgga
    36001 tccaccatcg aaaccggccg gttcttccag ttcggcgtgg cgatgatcgt gtccatgccc
    36061 aacccgaatg accgagccag cttcttcaca cgatcgactt ccgtttcgga ccagttgatg
    36121 tgttcgccga cgagttcgac ggactggacg ccggagcgcg cggcggcctc cattttttcg
    36181 tcaaaggtcc ccttgagcgt ccaaagcatc accgaagagg tgatcttcgc ctttttcggc
    36241 ggcgccgtgg tctgtcccga ggccgtccgg cctgccaggg ccgctccgga agcggccaca
    36301 aaggtcctgc gatccataca tgctattcta gagaatcatc agccatcccc ggggtttcgc
    36361 atgcgccagt tgaggtacac cacgcggagc ggcatcgatg tggtccgcac cgtctcgcga
    36421 gtgcccttcc gcaagggtct tgaccacctt ctgcgcgaac tcgattcatt ccgcggcatt
    36481 tatctttctt ccggctacga atatcccggc cgctattcac gttgggacat cgcctcgacg
    36541 aggccgccgc tggagatcgt ggcatatggc cggcggatcg agttccgggc gctgaacgtc
    36601 cgcggcgaaa tcctgaccca gatgctgtac ccggctcttg ccggccaccc acactgggag
    36661 tcgctggagc cttgtggggg ttctctggta ggccggctga aaccgctgcc cgagttgttc
    36721 ccggaggagg agcgcagcaa gcagccctcg gctttctcga tcctgcgggc tctgatcgaa
    36781 gaatttcgga acgaagagga ctcgcgcctg gcgctgattg gcgccttcgg ctacgatttg
    36841 ctcttccagt tcgacccgat ccggctgcgg ctgccgcgga gcgggcaaaa ggacctccac
    36901 ctgttccttt gcgacgacat ctatttcatg gatcgcaaga aggagcagat cgaacgttac
    36961 cagtacgatt tcgagaagga cggactctcc acactggcgc tcgatcattc ggcggagccg
    37021 gtttccccgc ctgggcgatc atcgattgaa ggcatcgcct ccgatcacgc gccggaggag
    37081 tacatggcga atgtcgagaa ggttcgccaa ggcatgaagc agggcgacta ctacgaggtc
    37141 gtgctgcgcc agacgttccg cgcgccttat gatggcagcc agtccgagct gttccggcgc
    37201 atgcaggaga ccagcccgag cccgtacgag ttctttctcc agttcggcga tgagcaactg
    37261 atcggcgcgt cgcctgagat gttcgttcgg atcgaggggg accgcgtcga gacttgccca
    37321 atctcaggca ccgcccgaag gaccggcgat ccgatgcacg acgccgacag catccggcag
    37381 ttgctcaatt ccgccaagga agaatctgag ctgacgatgt gcaccgacgt ggatcgcaat
    37441 gacaagtcgc gggtttgtgc gccgggctcg gtaaaagtca tcggccggag gctgatcgaa
    37501 tcctacgccg gcgttttcca caccgtcgat catgtggaag gaattcttca accgggctac
    37561 gacgcgctgg atgcatttct cagccacatg tgggcggtta cggtcatcgg agctccgaag
    37621 aaggc
//
LOCUS       DATIFQ010000462        16333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_348664, whole genome shotgun sequence.
ACCESSION   DATIFQ010000462 DATIFQ010000000
VERSION     DATIFQ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16333
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348664"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..742
                     /locus_tag="VLE22_23350"
     CDS             335..742
                     /locus_tag="VLE22_23350"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB17406.1"
                     /translation="MLSSTAVATAVATAIERRRGKRYLLRLACRVTSWDGHFEEMQGT
                     TADISRTGVRVVFPDEVALPMLHIGETACVLVDLPHSPYFTPRQLECMCRVVRIGDED
                     PTGPTAAFEIVRTRVRERNGGLVDGDPITKFLQ"
     gene            complement(1843..2568)
                     /locus_tag="VLE22_23355"
     CDS             complement(1843..2568)
                     /locus_tag="VLE22_23355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17407.1"
                     /translation="MAGVAVSEEKQARPWLRMKNESAKTYSHFQVYLRLGPNNRSFGK
                     TSEEVRCSVRNIERIASRWNWVERADAWDRHNEELNLARFEKLRARAAQRRLERELQR
                     REEAEQVKNLLLERAQRMLTWPLEEEMVTEKTEITADGQRLHITNRVVKPVKWDMNTA
                     VRVVEALDRMQHASVEDALKAQETAGIPAEQIAERHFTLHSAEQVQQPTDTSMDDAPR
                     EGNWTVDEGGDGVHRPTEPRILW"
     gene            3879..4610
                     /locus_tag="VLE22_23360"
     CDS             3879..4610
                     /locus_tag="VLE22_23360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17408.1"
                     /translation="MLAMILSLPLSASDRQKGIYVSTSDSILPHIADGNGWKTLITLV
                     NTRSVDVQYTLAFHSSPAGQPWKLNIAGLGAVSTISGTIPIGGSVFFETTNPPGATSV
                     GWAELEDNYDRVAGMAVFRFSPEGLQDTEAVVPFANKYDTDFYIPFDNRAGFVTSIAI
                     VNPSSHDVAEVFIEFRDLKGQRILQETITLQPLEHLPFSTIQRFSATADKNGVIEFTS
                     VRGVSALGIRFNPRFSFTSVHALSK"
     gene            4995..5198
                     /locus_tag="VLE22_23365"
     CDS             4995..5198
                     /locus_tag="VLE22_23365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17409.1"
                     /translation="MISERIFEAPSTKERLETGFALLQSILEQRLAAGDSGFAWGIER
                     CIVDCELDQLETDLYSLRPPQAS"
     gene            5322..5966
                     /locus_tag="VLE22_23370"
     CDS             5322..5966
                     /locus_tag="VLE22_23370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17410.1"
                     /translation="MPRKNAFHPPLRTAFVAVLLLAQASLILSAAEIPKGTHVLLRMV
                     NSVTTRTARVGDQVYMRTASPMGVSDRIVVPVNSYVQGVVTSSRRGGRVSGRAELGLR
                     LDTLTLPAGRSFRLSCGLQSVDAQGSSQRAEPEGAIDQGPDHGRDAARILITAGTGAA
                     LGAVVDQTVQGAAIGSGAGGAVGLATVLLTRGREVELRQGATLDVILDRPLLIE"
     gene            complement(5987..7015)
                     /locus_tag="VLE22_23375"
     CDS             complement(5987..7015)
                     /locus_tag="VLE22_23375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17411.1"
                     /translation="MTRLTLAGLLALTALPVGMAQTLSLVSARDGIEGSFTTPQAVYA
                     DHQQVYLASNDGRLFILEHNDAKGFPLVAIVNVSWAPLTAVRGDNKYLFVASADGTLY
                     VFNKRQPFSLVKVLTLSSFGLSALEVRQKQTRGELLVAHGQGQLAADEQRVYLSELNQ
                     GELGFRMDKNTFAILQQYGQTLESALTVVFDRASGARIGAIPNPDDLLGRVSQVALYA
                     DIQMLFQTTPGCCGRGIWIYDDALNLVQFIDRPSTNTVALSADKRWLIAGNENGTVDV
                     FDLSPNAPVLAASINLREVTSHPEPEAIEIRSLWVDDSGWILAASSWGNDLNRSPALP
                     SFFVLRLQ"
     gene            complement(7250..8479)
                     /locus_tag="VLE22_23380"
     CDS             complement(7250..8479)
                     /locus_tag="VLE22_23380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17412.1"
                     /translation="METSPALSREHGERAAALLLQPASFLTLVLAVVLIACGRSPANV
                     EQASPAPQLSGSAPPRSAADDLGRFLAGLPGDKASQFTSLERKDGWNVHRRELDSLWR
                     KVENEWLPAMRQFQKKELSGLPAGTSLVFYPFSGPDVLMATVFFPDSPVYVMVGLEPA
                     GTLPTPKQVAKGDLSKYLTEVRDSVSSELERSFFITREMDREFRGQVTDGLLPPILLL
                     LARTHHTILGYQYVRLDADGRVVERAADYKAPGRIGDKGVEIDFRSDANGPAQKLFYF
                     SVNLSDERLRENKPFLAYLEELRGMTTFLKATSYMPHDPAFSIIRDRVLVGSSVILQD
                     DSGIPYRFFDTAGWQVQLYGEYTRPYGSFRWREEPDLRKAYESGGAKPLGFHIGYGFS
                     RVPSNLLLARRKPAGSQ"
     gene            complement(8646..9035)
                     /locus_tag="VLE22_23385"
     CDS             complement(8646..9035)
                     /locus_tag="VLE22_23385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17413.1"
                     /translation="MKLGIRLVKIASLYMLLGLVMGVAMAVSHDFTLSSVHAHITLLG
                     WATMAITGIVYTVLPRCGESRLSAWHFWGHNIGLPVMMISLGLFTYGYTGAEKVIGVG
                     SILVLLSLITFTINLYLHGATGRASQG"
     gene            9149..10474
                     /locus_tag="VLE22_23390"
     CDS             9149..10474
                     /locus_tag="VLE22_23390"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB17414.1"
                     /translation="MQRRHFLGSLPLAIAAGGAMRAEVEPKAPVVTNPRATAGDPVEP
                     KWESAITLTVGPQKADLTGSDHKVIQAAVDYVARLGGGTVHILPGTYRMRNAVYLQSR
                     VRILGSGDESVLIKEPSVKTNLASNSDWYDQEVTLEDAAGFEIGDGVVLRTRNPHNGS
                     FDTYKRTLIARSGNRFKLNEMLVENFWVREGATAATLFALFDGMHVSDVTIENITLDG
                     NKSANEFLNGNYVGCIFLRESNRIHMRRVAARNFNGDAISWQVCHDVRVENCHSHDNG
                     GYGVHPGSGSQRTVVVNNRLERNEIGFFFCWGVRWGLCEGNTILDNRKFGVSVGHNDT
                     DNLICKNDIQRSGQVGVLFRQEHGPTFSPNRNRLEENRIIDSGAEKGIGVKVEGQTQS
                     VEIARNEIRENRQQAERVGILIEADATDIRLSGNIIEGFAQPVADLRKT"
     gene            10489..12228
                     /locus_tag="VLE22_23395"
     CDS             10489..12228
                     /locus_tag="VLE22_23395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17415.1"
                     /translation="MRSLFVAVTGFWLCAGASAAELPGKYFQLMQAGIAPVENRFAEG
                     DPGDLAMLEKQPGWRHFPHALLVAAVLYTKRNPANTRYHDAKMLALAKKIGDLVAKEN
                     QTGAFTKRLDHHRDLYMWLEAYRVLEKDLEPDRRAAWRSEIEKNLEELSADVAPRVDF
                     GAYISPFIGTSPNHYSLWSSTLHMGGIVFGNRAWRDLGARVMHRFVAEEQAPDGYWGE
                     HDITGPTTGYDNLTMTAAALYYENSKDPAALEALRRSNDFHTHFTYPDGRPVEVVNDR
                     NRYWEVNAWGHFGFSHFPDGRRFAAFLASHFEEDHLDIEDLGRVAQSALYYHNGPSSP
                     IPQDSKSDVYRMKRATAGIRKSGPWVVCLSGLISTQAPTNQFYLDRQGHVSVFHEKAG
                     LIITGANSKRQPELATFWEKLEGETYHMPQAARLRMAGTEDRLSLAYNSFFSDLYVPA
                     PKGPDVELKFRVTPKGRRDDGSLNLQVCLKAGETLETGSGKQLTVGTDNIELGAAELG
                     GWIRHRSWTLKLDPGARLSWPVYPFNPYGNGPEKSLRNAVAVVSVPIQANRPSGQAYG
                     AGVQEISFTVRVE"
     gene            12412..13794
                     /locus_tag="VLE22_23400"
     CDS             12412..13794
                     /locus_tag="VLE22_23400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17416.1"
                     /translation="MTRATPLAAAGNAPDAPWSGAAVVRKVYLTARQASWPRPDMNLD
                     QEIREIEANLAEVERRYPGRIRFTGGEVLRDPNDVAAWAGNMGDADAVLAFNMTNGVL
                     RMLEKVVDSGRPMLLFARPYAGHDWTHCSAFGQMGKKADVIASSDYAELDPYVDAFQT
                     IHHLRHSKVLVMAPERWRPNGDGYRQQFGTTFQSPTYLDLKAGYEAADVKEAQKLADE
                     FIRDAAKVVEPPREDIVDSMRLYLAIQNMMQHENANAITIDCLGGFGRRELPAYPCIA
                     FSKLNDTGQYGVCECDLESTMTQLLVTSFSAKPGFVSDPVFDTSRNEVIHAHCVSATA
                     MEGVGMPGAPYMVRTHLEDHKGVSMQVLLDAKGPVTVARFTGPDKLLVSTGEAAGNVD
                     DERGCRTKIRTKVTDARKFLRNYALVRTSGVQPSNTRDLLHRVVFYGDHVEMIERLGR
                     LTGFQVMHEV"
     gene            complement(13858..14856)
                     /locus_tag="VLE22_23405"
     CDS             complement(13858..14856)
                     /locus_tag="VLE22_23405"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HSB17417.1"
                     /translation="MNTRLVICLAVVALCPGMSGDNRFVPGRPVHTYSIVARDPATGE
                     LGAAVQSHWFSVGSLVIWAEAGVGAVATQSFVDPAYGPLGLELMRGGKTAGQALAGLV
                     STDPSRDVRQVAMIDANGNVAAHTGTKCIQSAGHIIGKGYSVQANLMRNDRIWPAMSA
                     AFENAKGDLAERMLAALEAAESAGGDIRGRQSAAIVIVKAKSSGRPWADRMMDLRVED
                     HPQPLVELRRLVKLHCAYEHMNQGDLAVEHNQAERALKEYAAAETLFPDSLEMKFWHA
                     VALVNMKRVDESLPLFREIFTHDRSWAELVPRLPKSGLLKDDPVLVRRILSVAPKQ"
     gene            complement(14883..15365)
                     /locus_tag="VLE22_23410"
     CDS             complement(14883..15365)
                     /locus_tag="VLE22_23410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17418.1"
                     /translation="MIKTVTFTVAAAYLAAGGWFLARAGPEVWDYQAWDEVSALLARE
                     TRAGDAVVVQPSICVAALQYYYKGPAVTYPPKWIEGWSPAPEELAASIAKKHGTVWLV
                     LSSFENPSSFVSSFTERPERRRRVATADYAARIERAGCLIREAKRFLRATVIGFECLR
                     "
     gene            complement(15393..16205)
                     /locus_tag="VLE22_23415"
                     /pseudo
     CDS             complement(15393..16205)
                     /locus_tag="VLE22_23415"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
BASE COUNT         3301 a         4885 c         4976 g         3171 t
ORIGIN      
        1 cttctccaac tacctcgacc taggatggac gaagcgccac gaacgatcag cctgcgagcg
       61 acagcactac cgcccaacgt ggccgttcct gcgcctccca gcctcgccac gcgcttgtgc
      121 cgctccgtcc gggcctcgcg ccctgcgtca gcgtatgggc tggaaggtca accacgagcg
      181 gctgggtgcg tgccgaccaa atctcgggaa attccgagac agcaagaagg tttcttgttg
      241 cacagattgg ttatcataca agccgataac cctgtaccaa atcgctgacc gaaagatagg
      301 gcgcgttctc gaagtcagtg atttattctt gggtatgctc tcttcgaccg ccgttgcgac
      361 cgccgttgcg accgccattg aaaggcgcag aggaaagcgc tacctgctgc gactcgcctg
      421 ccgagttacc tcttgggacg ggcacttcga ggaaatgcag ggaaccaccg ccgacatcag
      481 ccgaacgggt gtgcgggtcg tcttcccgga cgaagtcgcg ctgccgatgt tgcacattgg
      541 agaaactgct tgcgttctgg tggacctgcc gcatagtcct tacttcactc cacggcaact
      601 cgaatgcatg tgcagggtcg ttcgcattgg cgacgaagat cccacaggcc cgacagccgc
      661 cttcgagatt gttcgcaccc gcgttcgcga gcggaacggt gggctggtag acggagatcc
      721 catcaccaaa tttcttcagt gaagcgagga tgggaagggg ccgtgcggcg atggctagca
      781 gggcaaaaaa aaggccaccg gcttggcgtc ccgatggcct ctctgggagc agaagattta
      841 ccggcgaaca ggacggcttt actatgatgc gccctacctc tggcgcgcgc cggttcgcgt
      901 atcaaaaatg ctacgctcct aaatcccgtc ttgatgtctc tttttgaaca gtccaaccgg
      961 cttttttttg ttccggggcg ggcggcacac gtcggggcgg catgagcgcg actggaaggt
     1021 ttggaagagg gccatcctgc cgtcgatcag gaacggttga attgaaaggc agcctcgttt
     1081 cgcaagggtc gtgcagtgaa ccgcgatttt ctctcacaaa aaatggccac cgaacttctg
     1141 agattcgatg gcctatcctg agtcttccga tttggtcttg tctccgggtt aggcggtctc
     1201 gacactcttg ggcttgtgcg acgcgcccta cccgtagctg ccgaggagat gttacgctcc
     1261 tgcaatcctg ttttcaagtg cttttggaac agtcgagcaa gtttctttcg atccggcacc
     1321 gttgcacggg cggctcgcgt tgaggcgatt gggcggcggc ttcaagacag aaaaaggcca
     1381 ccgctggtcg accggtggcc taatgtggag gagtttcgta tcttcgtaca gtctcggaaa
     1441 tgatcctact gttccgtttc ttctgtcaag ctcatttttc acatctaggg atgcggcaat
     1501 caccacggcg gctgaacacg aaaaaggcca gtgaatcgga ggcgttcact ggcccgctcg
     1561 caatcataat cttcggtcag cgggttggtg cccgatggcc tcgtgagggc aaggtgtagg
     1621 aattagcgga cctgctgagt ctctctatga tccaccctac ccaagttgcg ccaaaattgg
     1681 cgtggaaagg atattacgct cctaaactct gtttgtcaag tcaactttat ttcgctccgg
     1741 gaccggggcg cgggcggctg aatgggctgg aaggacaacg cgtgccagcg cccagcactg
     1801 gaatcctgca ggatcacgcg cttcttctgc gccgccaagc cttcaccaga ggattcgcgg
     1861 ctctgtcggc ctgtggacgc catccccgcc ctcgtccacg gtccaattgc cttccctcgg
     1921 cgcatcgtcc atcgaagtgt ccgtcggttg ttgcacttgc tccgctgagt gtagcgtgaa
     1981 gtgccgctcg gcgatctgct ctgccgggat gcctgcagtc tcctgggcct tgagcgcatc
     2041 ctcgaccgag gcgtgctgca tacggtccag agcctcgacc acgcgcactg ccgtattcat
     2101 gtcccacttc accggcttga cgactcggtt ggtgatgtga agacgctggc cgtctgccgt
     2161 gatctccgtt ttctcggtga ccatctcttc ttccagcggc cacgtcagca tgcgctgagc
     2221 gcgttccagc agcagattct taacctgttc ggcctcttcg cgccgctgta actcgcgttc
     2281 aagacgacgc tgggccgctc tcgcgcgcag cttctcgaag cgggcgaggt ttagctcctc
     2341 attgtgccgg tcccaggcgt cggcgcgctc aacccagttc cagcgagagg cgatccgctc
     2401 gatgttcctg accgagcacc gaacttcctc cgaagtttta ccgaagctcc gattattcgg
     2461 cccgaggcgc aagtagacct ggaaatggct gtaggttttg gcactttcgt tcttcatgcg
     2521 cagccaaggc cgcgcctgct tctcttcgct cacggccaca cccgccatga cccggtccgg
     2581 cgcaattcgt cccgaattcc gtccggttgg ccctcccgcc gcccgagtct cactgggatt
     2641 tgatttctag atccgattat gccctggatc gcggcgcaag aaaagccctc tctacccgtt
     2701 ctgtcgctcc agagacccgg cagcaacgca cacctgcggc caagacctgg gggaccgggc
     2761 tggcgggggg ctgcggtccg ctacggcctc cctctgttcg cttaccagcc tcacgccacc
     2821 ctgattgtta ggactgccgt gtaccaatta tgtaccacgg gtctgctttt ttgccttttc
     2881 tggcatttct gcgcccttag ccggtcaata acttgcgtgt tttcaatcgg gttcagtaat
     2941 tcgaccccct cctccagcac catcattgta agggagttgt acgatcctga cgctcctcgt
     3001 tctggacttc ggaaggggcc agtgtgcccg gaagtgtgcc cagatgctcc aagtggtgct
     3061 gtacccactc ggcagcgggg tctagatgcc ggtcgctcac gatattgggg gagttgtcga
     3121 tccaacccgc cacgtctcgc agcccgcagc gtccgatgag ccccgtagaa acccgcagcg
     3181 ttgtgcattc attcggaact actgctccag taccgagcac gcccggtact atccctaacc
     3241 gtgttctagg cccatttgcg aacccagcct gccggttaac gatgaatagg aaaggacgcc
     3301 gtatgggcgc aggcggaggg gatcaccaat gagagtggcg gtgaagatga caaatcagaa
     3361 accgagaaca gcgcggtcga tgggcctcag tgatgaagag gtcgccaaga tgctgggcat
     3421 ccgcctagag cggcttgtca atgcccttga cgaagcaggg ctatgtcgat gccctcggcg
     3481 aagtggggct atgtcgatgt ccgaagaagg tgacaccggt gtcggccctt tcggctatcg
     3541 ccgggaggat tctgttcctt cgacttcagg cgggcctgat tctgcacgat gaacgccttg
     3601 atctcgtctg ccatcgacaa cgtctcggcg gcggtgtgcc tcccggtgta cccgaagccg
     3661 ggtggcgccg tgattctctg tgttctacaa gccgctgaaa atggggcttt gccaccggcc
     3721 ggagccgcgt ccaatcaccc cgagatggga ctcaaaatcc cgcgatcttt tactggtcat
     3781 gggggttcaa ccccctccag caccataatg acttggcggg aacttctgga agggttttca
     3841 ctatgagatc gtacgtgtgg tcgttcaggc gggctgcgtt gcttgcgatg atcctctcgc
     3901 ttccgctttc agcgagcgat cgacagaaag gaatttacgt ctcaacttca gattccatac
     3961 ttccgcacat tgccgacggc aacggttgga aaacgctcat cacattggtc aacacgcgtt
     4021 ctgtggacgt tcagtacact ctggcgttcc attccagtcc ggcgggtcaa ccgtggaaat
     4081 tgaacattgc cgggctcggt gctgtcagca cgatctccgg aaccatcccg atcggcggat
     4141 ctgtgttctt cgagactacc aacccacctg gcgcgacatc cgtaggctgg gcagagcttg
     4201 aagacaacta cgatcgcgtt gcgggcatgg ccgtctttcg attttccccc gaaggcctcc
     4261 aggacacgga agccgtcgtg ccgtttgcga acaaatacga tactgacttc tacattccat
     4321 ttgacaaccg agccgggttc gtcacctcga ttgcaatcgt aaatccctcg tcgcacgatg
     4381 tggctgaggt attcattgag ttccgggact tgaagggaca gcgaatcctt caagagacga
     4441 ttacgttgca gccgcttgag catcttccgt tttcaactat tcagagattt tccgcaaccg
     4501 ctgacaagaa tggcgtgatt gaatttacaa gcgttcgggg agtctccgcc ttaggaatcc
     4561 ggttcaatcc ccgcttttcg ttcacgtcgg tgcacgctct gagcaagtga ggcgagtctg
     4621 ggatcaactc ggccgccgcc tgcccagcga gtgccggcag gtagtactgg gttcgcccgc
     4681 ccttgtagag ccagtcaccg gcgtgcttct cgcccttgcc tttggtaccg catacagtgt
     4741 tcgccttcca ccttcggtgt gctcgtccgg gctgccacgc ggtgttcgaa ccgggatgcg
     4801 cggatccagc ctgtttcagg ttgacacgcc ggtgagacac caaagtgttt caggttgtgt
     4861 ctcatacgtt tccccaacag acctgaacga aaacaagtct cccattctca acatgctaag
     4921 gttctccccg catttggcct gagccctgct ctatcatccg tagaacttaa tctctcaccg
     4981 gggggagaca cccaatgatt tccgagagga tcttcgaagc gccctcaacc aaagaacggc
     5041 ttgagaccgg cttcgcactc ttgcagtcta ttctggaaca gcggctggcc gcgggggatt
     5101 ccgggtttgc ctggggcatt gaacgctgca ttgtggattg cgaactggac cagttagaga
     5161 ccgatctcta ctcgctcaga ccgccgcagg ccagctagct caggcgctca aaaggacccg
     5221 acgctccaga aattcccgtt cgtaacttag aagatcgggg tgccgtgctt ggtcggctat
     5281 gtcaacggca ggcctagaat gagttcagag ggggaacggt tatgcctcgg aagaacgcct
     5341 tccacccgcc gctgcgaaca gcattcgtag cggttctcct gcttgcgcaa gcctcgctaa
     5401 tcctgagtgc ggcggaaatt ccaaaaggta ctcacgtcct gctgcggatg gtgaactccg
     5461 tcaccacccg gaccgcccgg gtgggcgatc aagtctacat gagaacggca agccccatgg
     5521 gagtctctga caggatcgtc gtgccggtga acagctacgt gcagggcgtc gtcacgtcct
     5581 ccaggcgcgg tgggcgagtc tccggccggg cggaactggg ccttcggctg gacacattga
     5641 cccttccggc ggggaggagc ttccggcttt cctgtgggct tcagtcggtc gatgctcagg
     5701 gatcgagtca gagagccgaa ccagagggag ccatcgacca agggccggat catggccgcg
     5761 acgccgccag aatcttgatc acggccggaa ccggagccgc cctgggagcg gtggttgacc
     5821 agacggtgca gggggccgca attgggtcag gcgccggcgg cgctgtggga ctggcgactg
     5881 ttctgctcac gagggggcgg gaagtggaac tgcggcaggg agcgactctc gacgtgatcc
     5941 tggaccggcc cctgctcatc gagtaagcca cccgcacgat cgatcgctac tgcaatcgca
     6001 acacgaagaa cgaggggagc gccgggcttc ggttgagatc gttcccccaa ctggacgccg
     6061 cgagaatcca accactgtcg tccacccata gcgagcgaat ctcgatggcc tcgggctcag
     6121 ggtgactggt cacctcacgc aggttgatac tggccgccag gaccggtgca ttcgggctca
     6181 agtcgaagac atccactgtg ccattttcgt ttccggcaat cagccaccgc ttgtcggcgg
     6241 aaagggcaac ggtgttggtg gagggacggt cgatgaactg taccaggttc agcgcatcgt
     6301 cgtaaatcca gatgccccgg ccgcagcaac ccggcgtcgt ctggaagagc atctgaatgt
     6361 cggcgtatag agcaacctgc gacaccctgc cgagcagatc gtcagggttg gggatagctc
     6421 caatacgcgc gccgctggcg cggtcgaaca ccaccgtcaa ggcggattcc agcgtttgtc
     6481 cgtattgctg aaggattgcg aatgtattct tgtccatccg gaatcctagt tctccttgat
     6541 tcagctccga gaggtagacc cgctgttcgt cagccgccaa ctggccttgc ccgtgtgcaa
     6601 caaggagctc gcctctggtt tgcttctggc gcacttccag cgcgctcagt ccaaaacttg
     6661 acagggtcag aaccttcacg agcgagaagg gttgccgctt gttgaatacg tagagagtgc
     6721 cgtctgccga agcgacgaaa agatacttat tgtcaccgcg gacggctgtg agcggagccc
     6781 aactcacatt gacgatggcg acgagcggaa agcccttggc atcattgtgt tccaggatga
     6841 acaaccttcc atcattggaa gcaagataaa cctgctggtg gtcggcgtag accgcctgag
     6901 gtgtggtgaa actcccttcg atcccgtccc tggcggagac gagggagagt gtctgcgcca
     6961 tgcccacggg caaggcggtg agggcgagta gcccagcgag tgtcaacctg gtcatctgcc
     7021 cctccctact tatgatgtga ttgtagatca gttttcgtta cacctgctct cagagaacca
     7081 ctcttttctc agcgcaccgg cgcgaaacct ggttggcagg tccggagacc tacccgatcg
     7141 tcaagtaggg aatcgttgat cgacagtgtg gcgaaggagt cctgtcctgc gtcggcgcac
     7201 ttaccgctag gattttcaac aggctcctat tgtgccgagc ggaatcttac tactgcgagc
     7261 cggccggctt gcgcctggcg agcaggaggt ttgaagggac tcgcgagaag ccgtagccaa
     7321 tgtgaaagcc gagtggcttc gctccgccgc tctcgtacgc cttcctcaaa tccggctctt
     7381 cccgccagcg gaagctgcca tatgggcggg tatactcgcc gtacagttgg acctgccagc
     7441 ccgccgtatc gaagaagcga tacggaatac ctgaatcatc ttgcaggatt acgctgctgc
     7501 ccaccaggac ccggtcacga ataattgaaa acgccgggtc gtgcggcatg taggacgtcg
     7561 ccttgaggaa tgttgtcatt ccccgcaact cctccaaata cgcgagaaac ggcttgttct
     7621 ctctgagccg ctcgtcggac aagttgaccg agaagtaaaa gagcttctgc gccgggccgt
     7681 tcgcatccga acggaagtcg atctccactc ccttgtcgcc gattctgcct ggcgccttat
     7741 agtcagcggc gcgttcaaca acgcgcccat ccgcatccag gcgcacatac tggtatccga
     7801 ggattgtatg gtgcgtccgt gcgagcaaaa ggagaatggg cggcaacaac ccgtcggtca
     7861 cctggcctcg gaactcccgg tccatctctc gggtaataaa gaagctgcgc tctagttcgg
     7921 aagatacgga atcccgcacc tccgtcagat atttggacag gtctcccttg gcgacttgct
     7981 ttggcgtggg cagcgtgccc gcgggctcga gtcccaccat cacgtacacc gggctgtccg
     8041 ggaagaatac cgttgccatc aggacgtccg gaccgctgaa ggggtagaag acaagggagg
     8101 tcccggccgg tagaccgctt agttccttct tctggaattg gcgcatcgcc ggaagccact
     8161 cgttctcaac tttcctccag agcgagtcca gttcccggcg gtggacattc cacccatcct
     8221 tgcgctccag gctcgtgaac tggctagcct tgtcgcccgg caggccggca aggaatcggc
     8281 ccaggtcgtc agcggcgctg cggggaggcg ccgacccgga caattgaggc gcaggggagg
     8341 cttgctcgac gttcgcgggg gaccgcccgc acgcaatcaa gaccactgcg agaacgagcg
     8401 tgagaaacga tgccggttgc agcaaaagtg cggccgccct ctcaccatgc tcgcgactca
     8461 aagcaggcga agtctccatt cttgtctccg gaagagcttg agtatccctc acgcacgact
     8521 tgcgcttcaa gcggcaggct cacgagagta cgttgagcgg ccgcaatgtg cccgtcacca
     8581 tggagttaca ggaggacttc gaggatctct gcgcttcgtt gaacgggcgc agcgttgatg
     8641 cccgatcacc cttgggacgc ccgaccggtc gcgccgtgaa ggtacagatt gatggtgaac
     8701 gtaatcagtg acagcaacac aaggatcgac cccacgccga ttaccttctc cgctcccgtg
     8761 tacccgtaag tgaagagtcc gagactgatc atcatcacgg gcaaaccgat gttgtggccc
     8821 cagaaatgcc aagccgacag cctgctctcg ccgcaacgcg ggagcacggt gtacacaatg
     8881 cccgtaattg ccatggtggc ccaccccagc aaggtgatgt gggcatgcac ggaggaaagg
     8941 gtgaagtcgt gtgacaccgc catcgcgacg cccatcacga gtccgagcag catgtagaga
     9001 gatgcgattt tcaccaacct gattccaagt ttcatgtctt ccttcacctt cctaggactt
     9061 tgaggccggg gtccgaattc gtctgtgact gtggtcacga ggccgatggt ttgggataga
     9121 atcacggttg caactggagc caagtaccat gcagagacga cattttcttg gaagccttcc
     9181 gctggcaatc gccgccggcg gtgcgatgcg ggccgaagtt gaaccgaaag cgcccgtggt
     9241 gacgaatccc cgggccaccg caggcgatcc ggtggagccg aagtgggagt ccgccatcac
     9301 cctcacggtt gggccgcaga aggcggacct caccggatcg gatcacaaag tgatccaggc
     9361 ggctgtggat tacgtcgcac ggctcggcgg aggaacggtt cacatcctgc cgggaaccta
     9421 ccggatgagg aacgccgtat acctgcaatc ccgcgtgcgg attctcggta gcggcgacga
     9481 gtccgtgctg atcaaggaac cgtcggtcaa gacgaatctc gcctccaact ccgactggta
     9541 cgaccaggag gtgacgctgg aagacgccgc gggcttcgaa atcggcgacg gcgtggtcct
     9601 taggacgcgc aacccgcaca acggcagctt cgatacttac aagcgcacgc tcatcgcgcg
     9661 cagcggtaac cgattcaagc taaacgagat gctggtggag aacttctggg ttcgggaagg
     9721 cgcaactgcc gcaacactgt tcgccctgtt cgacgggatg cacgtctcgg acgtgacgat
     9781 cgagaacatc acgctcgacg gcaacaagag cgcgaacgag ttcctgaacg gcaactacgt
     9841 ggggtgtatc ttcctccggg agagcaaccg cattcacatg cgcagggtgg cggcccgcaa
     9901 tttcaacggc gacgcgatca gttggcaggt ctgccacgac gtgcgggtcg agaactgcca
     9961 cagccacgac aacggcggtt acggcgtgca tcccgggtcc ggatcgcagc ggaccgtagt
    10021 ggtgaataac cgactcgagc gcaatgaaat cggcttcttt ttctgctggg gagtcagatg
    10081 gggactgtgt gaagggaaca ccatactcga caaccgaaaa ttcggcgtct cggtgggcca
    10141 caacgatacc gacaatctga tctgcaagaa cgatatccag cggagcggcc aggtgggggt
    10201 gcttttccgg caggagcacg gtcccacgtt ttcgccgaac cgcaaccgtc tcgaagagaa
    10261 ccggatcatc gacagcggcg cggagaaagg catcggcgtc aaggtcgagg gacagacgca
    10321 gtcggtcgag attgcgcgca acgagattcg ggaaaaccgc cagcaggccg agcgtgtggg
    10381 tattctgatc gaagccgacg ccaccgacat ccgcctgtcc ggaaacatta tcgaaggttt
    10441 tgcgcagccg gtcgccgacc tgagaaagac ataggttcaa aggcacgcat gagatcacta
    10501 ttcgttgcgg ttacgggatt ctggctttgc gccggcgcga gtgccgcgga actacccggc
    10561 aaatacttcc aactgatgca ggcgggaatc gcacccgtgg agaaccgatt cgccgaaggt
    10621 gaccccggcg acctggcgat gctcgagaag cagcccggct ggcgtcattt cccgcacgcg
    10681 ctgctcgtcg cagcggtgct ttacacgaag cgaaatcccg caaacacgcg ctatcacgac
    10741 gcaaagatgc tggctctggc gaagaagatc ggagacttgg tggccaagga gaaccagaca
    10801 ggcgccttca ccaagcgcct tgaccatcac cgggacctct acatgtggct ggaggcttac
    10861 cgggtgctcg aaaaagatct ggagcccgac cggcgggcgg catggcgcag cgagatcgag
    10921 aagaacctcg aggagctttc ggccgacgtg gcgccgcgag ttgacttcgg cgcttatatc
    10981 tcaccgttta tcggaacctc tccgaatcat tactcgctct ggtcctcgac actgcacatg
    11041 ggcggtatcg tgttcggaaa ccgtgcctgg cgagacctcg gcgcgcgcgt catgcaccgg
    11101 ttcgtagccg aggagcaggc gcctgacggc tattggggcg agcacgatat caccggtccc
    11161 accaccgggt acgataacct gacgatgacc gctgccgccc tgtactacga gaacagcaag
    11221 gaccccgcgg cgctcgaggc cctgcggcgc tccaacgact tccatacgca cttcacctat
    11281 cccgacggga ggccggtaga ggtcgtcaac gaccggaacc gctactggga agtgaatgcg
    11341 tgggggcact tcgggttctc tcatttcccg gatggccggc ggttcgccgc ctttcttgcg
    11401 tcgcattttg aggaagatca cctcgacatc gaagatctgg gcagggtcgc gcagagcgct
    11461 ctgtactacc acaacgggcc gtcgtcgccg attccacaag actcgaagag cgacgtctac
    11521 cggatgaagc gagccacggc gggcatccga aagagcggcc cgtgggtggt ttgcctctcg
    11581 ggactgatca gcacccaggc gccgaccaac cagttctacc tggaccggca agggcatgtc
    11641 agcgtattcc acgagaaggc cggcctgatc atcaccggcg caaattcgaa acgtcagccg
    11701 gagctggcca cgttttggga gaaattggag ggagagacct accacatgcc gcaagcggcg
    11761 cgcctgcgca tggctggaac ggaggaccgc ctctcactgg cgtacaactc attcttcagt
    11821 gacctctatg tccccgctcc gaagggaccg gacgtcgagt tgaaattccg tgtgactccg
    11881 aagggccgcc gcgacgacgg atccctaaac cttcaggtat gcctcaaggc cggggagacg
    11941 ctggagacgg gatccggcaa acagctcacc gtcggcacgg acaatatcga actaggggcg
    12001 gcggaactgg gcggctggat ccggcatcgc agctggacgc tgaagctcga cccgggggcg
    12061 cgcctgtcct ggccggttta ccctttcaat ccgtacggga acggtcccga gaagagcctg
    12121 aggaacgctg tcgccgttgt ctccgtgccc attcaggcga acaggccttc cgggcaggcc
    12181 tatggcgcgg gcgtgcagga gatctcattc acagtgcggg ttgaataacg cgaccgggcc
    12241 tgctgccggg tatcaaatga cctgcagcca gcttgtaaag ggcaggcccg cttgacgccg
    12301 tgatagaatc cgagcgctcg gaggatttcg ggcaaggagg tcgaaatgtc tggaaggcag
    12361 cattctttca ctcgccggca gtttatggcg gcgtcagggg ccgctttgag catgactcgg
    12421 gcaacaccgc tcgcggcagc cggtaacgcg ccggacgctc cctggagcgg cgctgcggtc
    12481 gtccgcaagg tctatctcac ggcgcgccaa gccagttggc ctcgacctga catgaacctc
    12541 gatcaggaaa tccgtgagat cgaggccaac ttggcggagg tggagcgccg gtatccggga
    12601 cggattcgat tcaccggcgg agaggtgctg cgcgacccca acgacgtggc ggcctgggcg
    12661 ggaaacatgg gtgacgcgga cgccgttctc gcgttcaaca tgacgaacgg cgttcttcgg
    12721 atgctcgaaa aggtggtcga ctcgggcagg ccgatgctgt tgttcgcgag gccctatgcc
    12781 gggcacgatt ggacgcactg ctctgccttc ggccagatgg ggaagaaggc ggacgtgatc
    12841 gcgtcgagcg attacgcgga acttgatccc tatgtcgacg ccttccagac gattcaccac
    12901 ttgcgccaca gcaaagtgct tgtgatggcg ccggagcggt ggcgtccgaa cggagatggt
    12961 taccgccagc agttcgggac cacgttccag tctccgacct acctcgacct caaggccggc
    13021 tacgaggctg ccgacgtgaa ggaggcgcag aagctggcgg acgagttcat tcgcgacgca
    13081 gcgaaagtcg tggagcctcc gcgggaagac atcgttgact cgatgcggtt gtatctcgcc
    13141 attcagaaca tgatgcagca cgagaacgcg aatgcgatca cgatcgactg cctgggtggg
    13201 ttcggcaggc gcgagctgcc ggcgtatccc tgcatcgcgt tctcgaagct gaacgacacc
    13261 ggccagtacg gtgtttgcga atgcgacctc gagtcgacca tgacccagct tctggtgacg
    13321 tcgttctccg ccaagcccgg ctttgtctcg gacccggtgt tcgacacaag ccgcaatgaa
    13381 gtgatccatg cgcactgtgt ctcggcgaca gcgatggagg gcgttggcat gcccggagcg
    13441 ccgtacatgg tgcgtaccca cctggaggac cacaagggcg tgtcgatgca ggtgctgctg
    13501 gacgcgaaag ggccggtgac ggttgcgcgg tttacgggac cggacaaact gctggtttcg
    13561 accggcgaag cggccggcaa cgtcgacgac gagcgcggtt gcaggacgaa gatccgcacg
    13621 aaggtcaccg acgcgcggaa gttcctccgc aactacgcgc tggtccgaac ttccggcgtg
    13681 cagccttcca atacgcgcga cctgttgcac agggtggtct tctacgggga ccacgtggaa
    13741 atgatcgagc ggctggggcg gctgaccggc ttccaggtga tgcacgaggt gtagggcggc
    13801 ggacatccgg acttcctgcg acgagattca gcggattctc tcccacggaa cccgccgtta
    13861 ctgtttgggg gcgaccgaga ggatccggcg gacgagcacc ggatcgtctt tcaatagtcc
    13921 ggattttggg aggcgtggta ccagctccgc ccaactgcga tcgtgcgtga agatctcgcg
    13981 gaacagcggc agcgattcgt cgacgcgctt catgtttacg agcgcgaccg cgtgccagaa
    14041 cttcatctcc agactgtccg gaaacaacgt ctcggcagcc gcgtattcct tcagggcacg
    14101 ctccgcctgg ttgtgctcga cggcaagatc accctggttc atatgctcgt aggcgcagtg
    14161 gagtttcacg agccggcgta actcgaccag tggctgagga tgatcttcca cgcgaaggtc
    14221 catcatgcga tccgcccacg gacgcccgct ggatttcgct ttgacgatca cgatcgctgc
    14281 ggactgccgc cctcgaatgt cgccgccggc ggattctgct gcctcgagcg ccgctaacat
    14341 tcgctcggcg aggtcgccct tcgcattctc gaacgcagcc gacatggccg gccagatcct
    14401 gtcgtttcgc atgagattcg cctgcacgga gtagcctttt ccgatgatgt gcccggcgga
    14461 ctggatgcac tttgtgccgg tgtgcgcagc gacattaccg ttcgcgtcga tcatcgcgac
    14521 ctgccggacg tcgcgactcg gatcggtgga gacgagcccg gccagcgcct gcccggcggt
    14581 cttgccgccg cgcatcagtt cgaggccgag cggcccgtag gcgggatcga cgaacgactg
    14641 agtagcgacc gccccgacac ccgcctcggc ccagatcacc aacgatccga ccgaaaacca
    14701 atgcgactgc acggcggcgc cgagctcgcc ggtggccgga tcccgggcaa ctatggaata
    14761 cgtgtggacg ggacggccag ggacgaaacg gttgtcgccg gacattcccg gacagagtgc
    14821 gaccaccgcg agacagatca cgagtctggt attcatggcg gttcggccat tttacccccg
    14881 gcctaccgga ggcactcgaa gccaatcact gtcgccctga ggaaacgctt ggcctcgcgg
    14941 atgaggcatc ccgccctttc gatccgcgcg gcgtagtcag cagtagctac tcgtcgcctc
    15001 cgttcgggcc gctccgtgaa ggaactgaca aaggaacttg ggttttcgaa agatgagaga
    15061 acgagccaca cggttccgtg cttctttgcg atggaagcgg cgagttcctc cggggcagga
    15121 gaccatccct caatccattt cggtggataa gtgaccgccg ggcccttgta gtaatactgg
    15181 agggccgcga cacagatgga gggctgaaca acgaccgcgt cgccggcccg cgtctcccgt
    15241 gcgagaagtg ccgatacttc gtcccacgcc tggtagtccc acacctccgg accggccctc
    15301 gcgaggaacc atcctcctgc cgcgaggtac gctgcggcga ccgtgaacgt gaccgttttg
    15361 atcatccgcg gcgacctacc aagacgagtc ggtcacaggc cgatcaaaca ggcggcaatc
    15421 acacaggcaa ccggaaacaa gcccagtccg tagcggtagg cgaagtacga gagggtggcc
    15481 ttgatgaaca gagagatcgc cagcagtgat gatgcggtga gaatgactag aaacaggaat
    15541 accgtgcggc gctccaggaa caaagcgacg gcccccgtca cttgcagtgg gagccaaaga
    15601 gccaggggaa gcaatgttcc gaaggatagc gggccggcat acatgcccgg cacggcgaac
    15661 agcattgccg gcgaccagat ggcggaaaga ctcaccgcgc gggtcatacc gcccgagatc
    15721 atgtagaaga cgacccatgc gagccacaga acaaggagga cggacatcgt caacatcatt
    15781 cgcatcggtc gggtggcagg cctgtgcgtg ccgttgggtt tcaaaacgaa gcggtaccaa
    15841 agggcggcca gaatttgccc gccaagatgg aacacgaaga tggtgtgtgt agcgagaccc
    15901 agcgcggcgg aagcgccgta gagcatccag tgcagccgcc ggtccgtttg aaaagcgcgg
    15961 tacagacaga ctgtggagac tgccgaagca agcatcagga gtgtgtaggc gtttccgtcg
    16021 gagaaatagc ggagcgcgcc gggagtaaga acgaagaagg cgctggcgag tgctcccacc
    16081 gccggaccgc cggcaatcct tccgagagcg aagacggcga gcgtcaccag aaagccaaag
    16141 accgccgacg gcagccgcat accggccggg ctgtcatcga tcgaatacac ccagcggacc
    16201 acagcgaaga aggccggtcg ggtacgcttt caaatccatg gcggacttga cgagcccgcc
    16261 gaacgttggt tgcctcgcaa tgatgtcggt gagcgactcg tcgtaccaca cggaatggct
    16321 catgtggctc acc
//
LOCUS       DATIFQ010000463         8199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2073141, whole genome shotgun sequence.
ACCESSION   DATIFQ010000463 DATIFQ010000000
VERSION     DATIFQ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073141"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..1687
                     /locus_tag="VLE22_23420"
     CDS             338..1687
                     /locus_tag="VLE22_23420"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17419.1"
                     /translation="MSNHLKRREFLGVSAAGAASGALGGLALPEQAAAQASIGGRPIR
                     MGFVGVGNRGSYHLDIALGIDGLEVPAICDINPARLHSAAKWIQDAGKPAPRTYGRSR
                     TDFLRLCEKEELDCVICCTSWEWHAPVLLAAMRNGKNACCEVPLIQTLDEAWEIVETH
                     EKTGKWATMGFKSPHTTLTNLVYHGVIGDILHCEGGYVHDLRLVKFDPKEEPWRLQHS
                     VDRNGNLYPDHPTCALAPIMDVNHGDRFDFLVSMSSKSVMLNRYADLLYGPKHPYATR
                     PMKLGDYNATLLRTVNGKLYTLNHDTNTPHPREFTRIQGSKGVLFMPGRELGRFIYVD
                     GMEPAHEWQPADKFLEQYRHPLEKEYNPKPRKAIRGHGGGERTTPLFWNRLILALHEQ
                     RVTDYDVYDSVTSSAIIPLTEMSVAKRCQPIDFPDFTRGKWKTRLPFEVEYYKGKKA"
     gene            1701..2945
                     /locus_tag="VLE22_23425"
     CDS             1701..2945
                     /locus_tag="VLE22_23425"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17420.1"
                     /translation="MADKTVRLGFVGLGGRGSYHLDCALGIPGVEVPALCEIVDARLQ
                     RAKKWVEESGRPAPRAYGGGVKDFERLCAEEQLDCVICATSWEWHAPVCLSANRNGKH
                     AVSEVPIVLTVEEAWALVESYEKTGKWSTLALEQVLMESGGGTYLSLLNMIRQGVLGD
                     TIHAESGYLHDLRKVKFPGGTEPWRLAHSTKRNGNLYPDHPMNRIMPMMDINHGDRFD
                     FLVSSSSRSGTLNEYAIATLGKDHELARTKMAQGDYNATLLHTEDGKVVTLNYDTNTP
                     HPRELYRLQGTKGVYMDSRGIKGPWIYLDGITEPEHQWEPAAKYLDEYRPALLKSYNP
                     PPRKSIRGHGGQGRGTPLTWHLLIKALREGSEPYFDVYDSVTSSVITALTETSVANRS
                     RPVEFPDFTKGKWKTRKPLEVG"
     gene            complement(3142..4440)
                     /locus_tag="VLE22_23430"
     CDS             complement(3142..4440)
                     /locus_tag="VLE22_23430"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSB17421.1"
                     /translation="MTSDELFASAQRHLIGGAGAGGRYHPLLGRPLYVACGRGSRFWD
                     VDGNEYIDFFTGSGANFLGHGHPAICDAVRKALELGVVCNGETEYHSKLAEMVADAVP
                     CAEKVRFANSGTEATMAAIRIARGYTGKPGILKFEGHFHGMHDYLWYNCSGRAGEVRA
                     DGTVAPLPESDGMPEALAGLVHVAPFNDLDAFERAMRAHGGELAAVIMEPVSYNMGCV
                     PSDPEYLRAVRRICTEREVVLIFDEVLSGFRMCRGGAQEYYGVTPDLCTLAKALGGGV
                     PIAAVCGKSEIMSVLNPAGRTVMSGTYTGHLTAVMGAIACQTELARPGFFGHVNGLAD
                     RLYTGLGEAMRTAGVQGVVQGLGARFGIYFGAIGPVKNYSQVVTTDRAVEVRFLRGCL
                     SRGLYFHDYGHTMHHGFSSQHTASDIDEALNMIEDSLRGH"
     gene            complement(4437..5792)
                     /locus_tag="VLE22_23435"
     CDS             complement(4437..5792)
                     /locus_tag="VLE22_23435"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB17422.1"
                     /translation="MDRFAVMAFSILLCGALQAAPDVVLAITAARLLDGVRSAAIPSS
                     VVLIGADGGIAAAGPAGEVAVPSGVRVIRAEGMTLLPGLIDGHVHLAWDKTLYTTFSA
                     EDYKARHKRWNPDRALVRAGHHAQLALAAGVTTVRDCGADDFTILALRDAINAGDFSG
                     PRILASGRPVTTTAGHTYSGWGVDGITEVREAIRHLAGERVDFIKMIVSGGTTTPGTN
                     ISQSQYTLEEVRAAVQEAHRLGLQVAAHAIATESIRLAAEAGVDTIEHCSWIGRDPKT
                     TVTDEVAVDFMVKNGVRVDHAIIPRAYMFPDESAAPLTAEEDWWLKMLKIRWPFLHHM
                     RKRGVTVFLATDAGFRPWPGTQNWPGFQELARAVEIVVRHGGFSPADAIAMVTREPAK
                     ALRLDREVGTIESGKRADLILVAGDPLQDIRALRNVEMVFRDGRLVAQQGHIVLPGSG
                     R"
     gene            complement(5796..6083)
                     /locus_tag="VLE22_23440"
     CDS             complement(5796..6083)
                     /locus_tag="VLE22_23440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17423.1"
                     /translation="MVLVSTIFPGKPSYGACQLGDKTVLDVVEVVRAVRSESVKAAAM
                     LSDLRNAMWRSWDACGTARQAGGARSFGDKSIGRDDPGRVVFQRIVEALSG"
     gene            complement(6188..7594)
                     /locus_tag="VLE22_23445"
     CDS             complement(6188..7594)
                     /locus_tag="VLE22_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17424.1"
                     /translation="MANRVGAVAVFTLLAAVSVFSQQVQEVPGNLRSMIQKSQSFGLD
                     SYPVSLWSYTNLAEHGKHMDEAEVEEWAEAGFTVPQSPTFNPDDPEQKAHMLRLLDWA
                     QKRKMKLIVCDPRGYARKGKSGGVAPGYADGVRAAARDFGSHPATFGFHVGDEPGAEM
                     KEPFFESYRIQKQAAPNLHPFANLLPLYPGATARAGANTWAEYLDEYVRKSNADLISY
                     DCYTQMNPGTEGHHRYYENLKEYRAAAVRNGVPFWNTILSVGHFRYRCPNLDDIRWQF
                     NTTLAFGAHGISWFFYYMRHPHENYRRSPVDEHWNRTQTYEDLRVVQKSFHRHYGDLF
                     TRLATTRASFYPEAFGGGEVFAPNGLVSRLVPDREGHPLLVGEFVDLRGQRYFMIVNN
                     SVSESVRVAVTFPGEDVKVFSWNWSGKEVEGGAHSATGHPTRDERGLTISHWLAPGQE
                     AVYRVESASAAREPITAR"
     gene            7942..>8199
                     /locus_tag="VLE22_23450"
     CDS             7942..>8199
                     /locus_tag="VLE22_23450"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSB17425.1"
                     /translation="MPGRNEIESLLPLAPVVFEILLALADGERHGYAIKREVARRTDG
                     KVKLGPGTLYGAIKRVLSQGLIEESDTRPDPHLDDERRHYYR"
BASE COUNT         1593 a         2648 c         2438 g         1520 t
ORIGIN      
        1 tgctcttccg atctggtggc ggcctgacgc cacggctcag gatcgctgat cgccgccagg
       61 gcgctcaggc gcttgcgatc gcgttctttt acccctgctg cttgcagacg cccggaacaa
      121 tcatcggctt agcgagcggc gcggcgggtt ccatggacag ccgaacagtt tcgcggaatg
      181 gaactgcatc gattgcccgg taccgtgcgc taccctgcgc aagccgcatc agtcggatca
      241 tcgttaagcc atgtgcggga aaaccgcacg cacggtctga aaggagtttg ttgaaaattg
      301 gctggctata ttgcccgtac cgcgtcaaaa tctacctatg agcaatcatc ttaagcgacg
      361 cgagttcctc ggtgtttccg cggccggtgc ggccagcgga gcgctcggcg gcctcgctct
      421 accggaacag gccgctgccc aggcctcgat cggcggccgg ccgatccgca tggggttcgt
      481 cggcgtgggc aaccgcggtt cctaccacct tgacatcgcg ctaggaatcg acggactgga
      541 agtcccggcc atctgcgaca tcaaccccgc ccggctgcac agcgcggcga aatggattca
      601 ggatgccggc aagcccgctc cacgcaccta cgggcgaagt cgcaccgact ttctgaggct
      661 gtgcgagaag gaagaactgg actgcgtcat ctgttgcacg tcctgggagt ggcacgcccc
      721 tgttctgctg gcggcgatgc gcaacgggaa gaatgcctgc tgcgaagttc cactgatcca
      781 aacgctcgac gaggcgtggg agatcgtcga gacgcacgag aagaccggca aatgggcgac
      841 catgggcttc aagtcccccc acaccacgct gacgaacctc gtctaccacg gagtgatcgg
      901 cgacatcctg cactgcgagg gcgggtatgt ccatgacttg cggctcgtga aattcgatcc
      961 gaaggaagag ccctggcgcc ttcagcactc ggtggaccga aacgggaatc tctacccgga
     1021 tcatccgaca tgcgcgctgg cgccaatcat ggacgtcaac cacggcgacc gcttcgactt
     1081 tctggtctcg atgagctcga aatccgtgat gctgaaccgg tacgcggatc tgttgtacgg
     1141 cccgaaacat ccatacgcca cgcggccgat gaagctcggg gactacaacg ccacgttgct
     1201 tcggacggtc aatggaaaac tgtacacgtt gaaccacgac accaacaccc cgcacccccg
     1261 tgagttcacc cgcattcagg gttcgaaggg cgttctcttc atgcccggcc gtgagcttgg
     1321 cagattcatc tacgtcgatg gaatggaacc ggcgcacgag tggcaaccgg ccgacaagtt
     1381 ccttgagcag taccgccatc cactcgagaa ggaatacaac cccaagcctc ggaaagcgat
     1441 tcgcggacac ggcggcggcg agcggacgac accgcttttc tggaaccgtc tgattctggc
     1501 tctccacgag caaagagtga ccgactacga cgtctacgat tccgtgacat cgagcgcgat
     1561 catcccgctc accgaaatgt cggtggcgaa gaggtgccag cccatcgact tccccgactt
     1621 cacgcgcggg aagtggaaga cgcggctgcc gttcgaagtc gaatactaca agggcaaaaa
     1681 ggcataggag gacgtcggaa atggcagaca aaacggtgag acttggattt gtagggctgg
     1741 gcggacgcgg ctcgtatcat ctggactgtg cgctcggcat ccccggcgtc gaagtgcccg
     1801 cgctctgcga gatcgtggat gcgcgcctcc agcgggcgaa gaaatgggtc gaggagtccg
     1861 gccggcccgc gccgcgcgct tacgggggtg gcgtgaagga tttcgagcgg ctgtgcgccg
     1921 aggagcagtt ggattgcgtc atctgcgcga cctcgtggga atggcatgcc ccagtgtgtc
     1981 tctcggccaa ccgcaacggc aaacatgcgg tgagcgaggt tccgatcgtt ctaactgtgg
     2041 aggaagcctg ggcgctggtc gagtcgtatg aaaagaccgg caagtggtcc acgctggctc
     2101 tcgagcaagt gttgatggag agcggcggcg gcacgtacct cagccttctg aacatgatcc
     2161 ggcaaggcgt gctcggcgac accatccatg cggaaagcgg atacctccac gacctcagga
     2221 aagtgaaatt ccccggaggc accgaaccct ggcgcctcgc gcattcgacc aagcggaacg
     2281 gcaacctcta tccggatcat ccgatgaacc gaatcatgcc gatgatggac atcaatcacg
     2341 gagaccggtt cgacttcctc gtatcctcga gttcacgctc cgggacgctg aatgaatatg
     2401 ccatagcgac cctcggcaag gaccacgagc tggcgagaac caaaatggcg cagggggatt
     2461 acaacgcgac actgcttcac acagaggatg gcaaggtcgt caccctgaac tacgacacga
     2521 atacgccgca tccgcgcgaa ctctaccggc tccagggtac aaagggcgta tatatggact
     2581 cccggggcat caagggccct tggatctatc tcgacggaat caccgagccg gagcaccagt
     2641 gggagccggc cgcgaaatac ctcgacgaat ataggcccgc cttgctcaag agctacaacc
     2701 ctcccccgcg aaagagcatc cgtggtcatg gcggccaggg aagaggcacg ccccttacct
     2761 ggcacctgct catcaaggcg ctgcgggagg gaagcgagcc gtacttcgat gtctacgatt
     2821 cagttacgtc gagcgtcatc acggcgttga cggagacttc cgtcgccaac cggagccgtc
     2881 cggtcgagtt tccggatttc accaaaggga agtggaagac gcgcaaaccg ctggaagtgg
     2941 gataagcgcg accgaccggt caggtgcgcc gggcttgcgc gccggtgaac cggccgccgg
     3001 agtcctcgag cgtcagcaac tcgttcggcc aggaactcat ctcgcgttcc ttccggagca
     3061 ggtccctgtc atctggatga cggttggcgt gatcctatgc gcccacggcg cttattaacg
     3121 caattcatat tcgaagcgga tctaatgccc tcgcagcgaa tcctcgatca tgttcagcgc
     3181 ctcgtcgatg tcggaggcgg tgtgctgcga ggagaacccg tggtgcatcg tgtggccgta
     3241 gtcgtgaaaa tagagacccc ggctcagaca gccgcgaaga aagcgcacct cgaccgcccg
     3301 gtcggttgtc acgacctgtg agtagttctt gaccgggcca atcgcgccga agtagatccc
     3361 gaaccgcgca cccaagcctt gcacgacccc ttgaacgccc gctgttctca tcgcttcgcc
     3421 gaggcccgta tacaggcgat ctgccagccc gttgacgtgc ccgaagaatc cgggtcttgc
     3481 gagctctgtc tggcaggcga ttgctcccat cacggcggtg aggtggccgg tgtaggtgcc
     3541 gctcatgacg gtccggcccg cgggattcag caccgacatg atctccgatt ttccgcagac
     3601 cgccgcgatc ggaacgcctc cccctaaggc tttagccagc gtgcacaggt ccggcgtgac
     3661 cccgtagtac tcctgtgcgc cgccgcggca catccggaat cccgagagga cttcatcgaa
     3721 aatcagcacg acctcccgtt ccgtgcagat ccgccggacc gcgcgcaaat actccgggtc
     3781 ggaaggcacg cagcccatgt tgtaggagac cggctccatg atcacggctg ctagttcgcc
     3841 accgtgggcc cgcattgcac gctcgaaagc gtcgagatcg ttgaacggcg ccacatgcac
     3901 caacccggcc agcgcctccg gcattccgtc ggattcgggc agcggggcca ccgttccatc
     3961 cgcgcggacc tcgcccgcgc ggccggagca gttgtaccag agatagtcgt gcatgccgtg
     4021 gaagtggcct tcaaacttga ggattcccgg cttgcctgtg taaccgcggg caatgcggat
     4081 cgccgccatc gtcgcctcag tcccgctatt ggcgaagcgg actttttccg cgcacggcac
     4141 cgcatctgcc accatctccg ccagtttcga gtggtactcc gtctcgccgt tgcagacaac
     4201 accgagctcc agcgccttcc gcacggcgtc gcagattgcg gggtgcccgt gcccgaggaa
     4261 attagcccca ctgccggtga agaagtcgat gtactcattc ccatcgacgt cccaaaaacg
     4321 ggatccgcga ccgcaggcga catagagcgg acgcccgagc agcggatggt acctcccacc
     4381 ggccccggcg ccgccgatca ggtgtctttg cgccgacgca aaaagctcgt ccgacgtcac
     4441 cggccgctcc ccggcagcac gatgtgtcct tgctgcgcga caagccggcc atcgcggaag
     4501 accatctcga cattccgaag tgcccggata tcctggagcg gatcgcccgc cacgaggatc
     4561 aggtccgccc gtttgccgct ttcgatcgtg ccgacctcgc ggtcgagccg cagcgccttt
     4621 gccggctccc gggtcaccat ggcgattgcg tccgccggac tgaacccgcc atggcgaacc
     4681 acaatctcga ctgcgcgggc aagttcctgg aaccccggcc agttctgggt gcccggccag
     4741 gggcggaagc ctgcatcggt cgccaggaac accgtcactc cccgtttgcg catgtggtgg
     4801 aggaacggcc agcggatctt gagcatcttg agccaccagt cctcttcagc ggtcagcggg
     4861 gcggcgctct cgtccgggaa catgtacgct cgcggaatga tcgcatggtc cacccgcaca
     4921 ccattcttca ccatgaaatc caccgcgact tcgtcggtga ccgtcgtctt cggatcgcgc
     4981 ccgatccacg agcagtgttc gatcgtatcc acccccgctt cggctgccag gcggatactc
     5041 tctgtcgcga tcgcgtgggc cgccacctga agcccgagcc ggtgcgcttc ctgaaccgcc
     5101 gcacggactt cttcgagcgt gtactgcgac tgcgagatgt ttgtccccgg cgtggtcgtt
     5161 ccgccgctga caatcatctt gatgaagtcc actcgctcgc cggcgagatg ccggatcgcc
     5221 tcgcggactt cagtaatgcc gtccacgccc catccggagt acgtatggcc cgcagtcgtg
     5281 gtcaccggcc gccccgacgc taggatgcgc ggtccgctaa agtccccggc gttgatcgcg
     5341 tctctcagcg ccagaatcgt gaagtcgtcg gcgccgcaat cccgcaccgt cgtcacaccc
     5401 gcggccagtg ccagttgggc gtggtggccg gcgcgaacca gcgcccggtc cgggttccat
     5461 cgcttgtgcc gcgccttgta gtcttcggcg gagaatgtcg tgtaaagcgt cttgtcccac
     5521 gccaggtgta cgtgcccatc gatcaggccc ggcagcagag tcatcccttc ggcacggatg
     5581 acgcgcactc ccgatggaac cgccacttcg cccgccggtc cggcggcggc gatccctcca
     5641 tccgcgccga tgagcacgac gctcgacggg atggcggcgc ttcgcacccc atccagcagc
     5701 cgggcggccg tgatcgccaa cacaacgtct ggagccgcct ggagagcccc gcagaggagt
     5761 atggagaaag ccatcacagc gaatcgatcc atgtcttatc ccgaaagcgc ttcgacgata
     5821 cgctggaaga ccaccctacc agggtcgtcg cgcccgatac tcttgtcgcc gaaactccgc
     5881 gcgccccctg cctggcgggc agttccgcat gcgtcccaag acctccacat cgcgttccgt
     5941 aaatccgaca gcatcgccgc cgcctttacg gactccgacc ttaccgcccg cacgacctcc
     6001 accacgtcca gtacggtctt gtcaccgagt tggcacgctc cataggaggg cttgcccggg
     6061 aatatggttg aaaccaaaac cattggaccg ccatcagtgg ctctcgtcaa gcgcctgtga
     6121 ttggaacggc acctctgcgt cgccgggaca gttcgcgacg catctctctt gccaacccct
     6181 aactggatta cctggccgtg ataggttcgc gagcggcgga cgcggattcc acccggtaga
     6241 ccgcttcctg ccccggtgcc agccaatgag agatcgtcaa accgcgctcg tcgcgcgtcg
     6301 ggtggcctgt cgccgaatgc gcgccgccct cgacctcttt tccggaccag ttccacgaga
     6361 acaccttcac gtcttcaccc gggaacgtga ccgcaacccg tacactttcg ctcacgctgt
     6421 tgttcacgat catgaagtat cgctgtccgc gtaagtcgac gaattcgcca acaaggagcg
     6481 gatggccctc ccggtccggc accagccggg ataccaggcc gttcggtgcg aatacttcgc
     6541 ccccgccgaa cgcttcgggg tagaacgagg cccgcgtcgt ggccagtcgc gtaaagagat
     6601 ccccgtaatg gcggtgaaag cttttctgca cgacacgaag gtcttcgtac gtctgagtgc
     6661 ggttccagtg ttcgtccacg ggtgagcgcc ggtagttttc gtgaggatgc cgcatgtagt
     6721 agaagaacca cgagatcccg tgtgctccga atgcaagtgt ggtgttgaac tgccaccgga
     6781 tatcgtccaa gtttggacac cggtagcgga aatgcccgac ggacaggatc gtattccaga
     6841 acggcacgcc gttacggacc gccgccgccc ggtactcctt gaggttctcg tagtaacggt
     6901 gatgtccttc ggtgccgggg ttcatttgcg tgtagcagtc gtagctgatg agatcggcgt
     6961 tgctcttgcg gacgtactcg tccagatact ctgcccacgt attcgcgccg gcgcgggcgg
     7021 ttgccccggg atagagcgga agcaggttgg cgaacggatg caggttcggt gcagcctgct
     7081 tctgaatgcg gtagctctcg aagaagggtt ccttcatctc ggctcccggc tcgtccccta
     7141 cgtggaagcc gaacgtggcc gggtgactcc cgaagtcgcg ggccgcggct cgcaccccgt
     7201 cagcatagcc cggagctacg ccgccggact tgcccttcct cgcgtaaccc ctaggatcac
     7261 aaacaatcag cttcattttg cgtttctgcg cccagtcgag cagtcgcagc atgtgcgctt
     7321 tctgctccgg atcgtcggga ttgaaggtcg gactctgcgg cacggtgaag cctgcttccg
     7381 cccattcctc gacctccgct tcgtccatgt gtttcccgtg ctccgcgaga ttcgtgtagc
     7441 tccaaaggct gaccgggtag gaatccagcc cgaatgattg cgacttctgg atcatggaac
     7501 gcagattccc aggaacctcc tggacctgtt gggagaatac actcacggcc gccagcagcg
     7561 tgaatacggc cactgcgccg actcgattcg ccatgttcgt cacctttctt cccggccgga
     7621 actttaccga ctgttccccg gctgcctcaa aactatactc cactccgacg aaaccgaaat
     7681 gtgaacccgc ggatcatcaa ttcgccactc cgttgtgcca caggaccttg acgagggaca
     7741 cctggggccc tatgtgatgc aaacaagtag cattatccgc tcgatcggtt ctagcgttag
     7801 cggggatcta gtggcacact tggcggacat ccacggacat ccatgacatc cactacggcc
     7861 ttgcaatcgc ggcgcgatgt attgtatcat gatacgtact agtagtccgc atacaggatt
     7921 atggagtaaa gcgctgtgaa gatgccgggg cgcaacgaga ttgagagcct gttgccgctg
     7981 gctccagtcg tgttcgaaat tctcctggcg ctggccgatg gtgagcggca tggatatgcg
     8041 atcaagcgcg aggtcgctcg acgaaccgat ggcaaggtca aacttggacc tggcaccttg
     8101 tacggcgcga ttaagcgcgt gctctcacag gggctgatag aagagtccga tacccggcca
     8161 gacccacatc tcgacgatga acgacgtcac tactaccgg
//
LOCUS       DATIFQ010000464         6444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_540147, whole genome shotgun sequence.
ACCESSION   DATIFQ010000464 DATIFQ010000000
VERSION     DATIFQ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540147"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..2154
                     /locus_tag="VLE22_23455"
     CDS             139..2154
                     /locus_tag="VLE22_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17426.1"
                     /translation="MRSAAEGLLAVCFVWVALPACAENRHAVPGEHPRLFGSKNELRD
                     LAKRKPGAYQRVLGVALEREGGNHEQMISSALVYAISGDEGIGRQALELTQKYVDGPI
                     RTGHETFGRDLARCAIVYDLCWPLWTPEQRNRFHSYMNRTVDANLKSETSVFHNGWYS
                     YKQWGIGLAAYATYYENKRAPEILAALEKEFRSRVIPAFKLAGDGGGWAEGYYVNYWS
                     HDWLVFCDVALRCEGVDYFAMSPEFMGKRALASMFESYPGIGERNSRRPVPIGDSGGR
                     VFTGERDKALSARRILVNRFRDDPVHQVVHAFNETTPVSGSDVNAYKDFLWRDTSVRK
                     GDLKSFKLSHLSAGPGFVFARSSWEDDATYFFFQCGDRFTSHQQLDAGNFLIYKYDEL
                     IGNGGHFYSFASDHEVNYLVRTIAHNTILTMDPREVWPAIRGGKVTSNDGGQMHDWPH
                     HNGSVEDAAAWEKARKLYDIADILAFEDRGGYLYVAGDCSRAYSPKKLKSFTRQIVYI
                     RPGTFVIFDRVNTTEPAFRKTFLLQAMKKPEGTGRSFTITNGKGRLFVQSLLPDGAEV
                     KQVSGAELYRIGGRDYPPDHATGPASECRVEISPSSPRTVDYFLHVLTATDASVSSVP
                     RAAVTVGSDKVTVTLPGINLTFAKDRVGGEITIAGKQTPLPATVVAK"
     gene            2368..4302
                     /locus_tag="VLE22_23460"
     CDS             2368..4302
                     /locus_tag="VLE22_23460"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSB17427.1"
                     /translation="MKRSILPGGAFLLLIAGTAHAAGLPLNFTEALVATGISSPTAMS
                     FAPDGRLFVCQQGGQLRVIKNGSLLATPFLTVSVDSVGERGLLGVAFDPNFAVNHYVY
                     VYYTVPSTPRHNRISRFTANGDVAVPFSETVILELNDLTTATNHNGGAIHFGPDGKLY
                     AAVGDNATGSNSQTLTNLLGKVLRLNADGTVPSDNPFFNQASGNNRMIWALGLRNPFT
                     FAFQNGTTRMFINDVGETTWEEINDGIAGSNYGWPATEGPTSNPLYRAPLFYYGHGGG
                     PTVGCAITGGAFYNPATQNFPAGYTGKYFFADYCSGWIRVLETGGFTASDLASGISSP
                     VDLQVGPEGYLYYLARGNGGQVYKVWYTANQPPQITTQPANQTVAVGQTATFSVGASG
                     SPPLSYQWYRNGGVIPGATSPSYTTPPAALADNGAQFHCVVTNPFGNDTSSPATLTVT
                     SNTPPTATIIAPALGTVYSGGQTILYSGSGTDAQDGALPGSAFTWQVDFHHDTHTHPF
                     IPPTIGAAGGSFQIPNTGETSANVFYRIHLTVRDSGGLTHSTFRDILPRTVIITLASN
                     PTGLQLTLDGQPVTAPFAVTGVVGILRSIGAVTPQRLGKIKYTFQTWSDGGAATHTIA
                     TPATNTTYTATFKQGGGPPR"
     gene            4766..>6444
                     /locus_tag="VLE22_23465"
     CDS             4766..>6444
                     /locus_tag="VLE22_23465"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB17428.1"
                     /translation="MTVTNTATGFSRQATTDDAGYYSIPNLPDGAYDVSVSASGFKPV
                     TQKGVDIVINTVARVDVTLEVGAVTEAITVEASGALLQTTKADVNVNLGSRAIENLPL
                     PGYRNYQSLINLVPGATPTRYQNAVTDTPQRALTTNVNGQERGANNTRVDGSADILVT
                     MPHHTVYVPPVESIEEVNISTNNFDSEQGMTGGAAITVTTKSGTNDFHGSAFGMHNNS
                     ALRSLLWDENRAGVTKKPKGIRNIVGGSIGGPIKRNKLFFFTDWEGTFERVNSARLFS
                     VPTDDVRTGDFSKELGDQILDASGNSIMIPTTEGGLVPLQEGMVFDPYTGNLDGTGRS
                     VFSNNGRLNVIPTSRLNGPMTRLLDLVPHPNQAGDLNNYFNSGTTRFNRNNIDAKVNW
                     NRNDKHQLWFKYGVMTALVHGDFGLGEAGGECLCQGGGLGDGKTLVQIAGIGQTYTVS
                     PTFLIDGTLGWTRFGQSVEPPDLGTNYGSDVLGIPGTNGPDPRESGLPAFYMDDYSAL
                     GNPEGWNPLFRNDQSYTVNVNASWMKGSHEIRFGFDFVHHLMNHWQPELGEG"
BASE COUNT         1336 a         1988 c         1912 g         1208 t
ORIGIN      
        1 gtttccggac atcaatgaga ccgcgcacac ggtcgatgtg acgctcgtct tcaattagcg
       61 ccaaccgcca gccaggaacc ccgtccccgc tttggctatg ctactggttt gccgatgacc
      121 cggggaaatc acagcagcat gcgctccgcg gccgagggac tactcgccgt ctgcttcgtc
      181 tgggtggcgc tgccggcttg tgccgagaac cgccatgcgg ttccggggga acatccccgc
      241 ctgttcgggt cgaaaaacga gcttcgcgac ctggccaaac gcaaacccgg ggcctaccag
      301 cgcgtgcttg gggtggctct ggaacgcgag ggcggcaacc acgagcagat gatctcttcg
      361 gcgctcgtgt acgcgatctc gggggacgag gggatcggcc ggcaggccct ggaactcacg
      421 cagaagtacg tggacggtcc catccgcacg ggtcacgaga ccttcgggcg ggatctcgcg
      481 cgatgcgcga tcgtctacga tctgtgctgg cccctgtgga cgccggaaca gcggaaccgg
      541 ttccacagct acatgaaccg cacagtggac gccaacctca aatccgaaac gtccgtgttt
      601 cacaacggct ggtactcgta caaacagtgg ggcatcgggt tggccgctta cgcaacttac
      661 tatgagaaca aacgtgctcc ggagattctg gcagccctgg agaaagaatt ccgttcacgc
      721 gtgattccgg cgttcaaact ggcgggcgat ggcggcggct gggcggaggg ctattacgtc
      781 aactactggt cgcacgattg gctggtgttc tgcgacgtcg ccctgcgctg cgagggagtg
      841 gattacttcg caatgtctcc tgagtttatg gggaaacgtg cgctggcgag tatgttcgag
      901 agttatcccg gcatcggcga acgcaactcc cggcggccgg tcccgatcgg ggacagcggc
      961 gggcgtgtgt tcaccggaga acgggacaaa gctctctccg cccggcggat cctggtgaat
     1021 cgcttccgtg acgacccggt tcaccaggta gtccacgcgt tcaacgagac cacaccggta
     1081 tcaggctcgg acgtgaatgc ctataaggac ttcctctggc gggacaccag cgtccgcaaa
     1141 ggcgatctga agagcttcaa actgtctcac ctaagcgcag gcccgggctt cgttttcgcg
     1201 cgcagttcgt gggaagacga cgccacctac ttcttcttcc agtgcgggga ccgatttact
     1261 tcccaccagc aactggacgc aggcaatttt ctgatctaca agtacgacga actgatcggc
     1321 aacggcggac atttctattc ctttgcctcg gaccatgaag tcaactacct ggtgagaacc
     1381 atagctcaca acacgatcct gacaatggat ccacgggagg tctggcctgc cattcgcggc
     1441 ggcaaggtga ccagcaacga tggcgggcag atgcatgact ggccgcacca caacggctcg
     1501 gtggaagatg cggccgcctg ggagaaggcg cggaaacttt acgacatcgc ggacatcctg
     1561 gctttcgagg atcgcggagg ctatctgtat gtggccggag actgctcgcg ggcgtattcg
     1621 ccgaaaaagc taaagtcttt cacgcgccag attgtgtaca tccggccggg aacatttgtg
     1681 attttcgatc gtgtgaacac gacggagccg gcgttccgca agacgtttct tctgcaggcg
     1741 atgaagaagc cggaggggac gggtcggagt ttcacgatca cgaacgggaa gggccggttg
     1801 ttcgtacagt cgttgctgcc ggacggcgcc gaggtgaagc aagtgtccgg cgcggaactg
     1861 taccgcatcg gcggtcggga ttacccgccg gaccacgcga cgggaccagc ctcggaatgc
     1921 cgggtcgaaa tttccccgtc ctcacctcga accgtggact acttcctgca tgtcttgacc
     1981 gcgacggacg cctcggtgtc ctcggtaccg cgggcagctg tgacagtggg aagcgacaaa
     2041 gtcaccgtca cgctgcccgg catcaacctt actttcgcca aggatcgtgt cggcggcgaa
     2101 atcacgatcg cgggcaagca aactcccctg ccggcaaccg tcgtcgccaa atagcgattc
     2161 ccgcacgggc ctcggcgccc tgcaagacag acgcggtatt ggcccatatc atgtcaaagc
     2221 tcgtggaatg accgacaacc ccggccctac agtcgggcaa tgttaaccag ttaaccgagt
     2281 actattgcca ggatcgccgt gttcggttag catcggggta ccaaagaagc gcaaagcgtc
     2341 agtcgccgca aacctgggtt cctgcatatg aaacggtcaa tcttgcccgg aggagcgttt
     2401 cttctattga tcgccggcac ggctcacgcg gccggccttc cgctgaactt caccgaagcg
     2461 ctggtcgcta ccggtatctc ctcccccacc gccatgtcgt tcgcccctga cggcaggctg
     2521 ttcgtctgcc agcagggagg acagttgcgt gtcatcaaga acggctcgct gctggcgact
     2581 ccctttctga ctgtctcggt ggactcggtc ggagaacgcg gcttgctggg cgttgcgttc
     2641 gatccgaatt tcgccgtcaa ccactacgta tacgtctact acacggtgcc cagcacgccg
     2701 aggcacaacc ggatcagtcg gttcacggcg aacggggatg tcgcggttcc cttcagtgag
     2761 acggtcatcc tcgagttaaa cgacctcacc accgccacca accacaacgg cggtgcgatt
     2821 cacttcggtc cggacggcaa gctctacgcc gccgttggtg acaatgccac cggctcgaac
     2881 tcacagacgc tcacaaacct gctcggtaag gtgctgcggc tcaatgccga tggcaccgtg
     2941 ccgtctgaca atccgttctt caaccaggcg agcggcaaca accgcatgat ctgggccctt
     3001 ggcttgcgca accctttcac tttcgctttc cagaacggga cgacccggat gttcatcaac
     3061 gacgttggcg agaccacctg ggaggagatc aacgacggca tcgccgggtc gaattacggg
     3121 tggcccgcga ccgagggccc gaccagcaac ccgctatacc gggcgccgct tttttactac
     3181 ggccacggcg gcgggcccac ggtcggctgc gcaatcaccg gcggtgcgtt ctacaacccg
     3241 gcgacacaga atttccccgc cggctacacc ggaaagtatt tctttgcgga ctattgcagc
     3301 ggatggatcc gggtgctgga gactggcggc ttcaccgcca gcgatctcgc ctcgggtatc
     3361 tcgtccccgg tcgacctgca ggtcggcccg gagggctatc tctactatct ggctcgggga
     3421 aacggcgggc aggtctacaa ggtctggtac acggccaacc agccgccgca gatcacaacg
     3481 cagcctgcga accaaacggt agcggtcggg cagacggcga cgttctccgt cggcgcgtcc
     3541 ggctcgccgc cactgagcta tcagtggtat cggaacggag gggtgatccc tggcgcgacg
     3601 tcacccagct acaccacgcc cccggctgct ttggccgaca acggcgctca gtttcactgt
     3661 gtagtaacga acccgttcgg caacgacacg agcagcccgg cgactctgac ggtcacgtcc
     3721 aacacgccgc ccacggccac aatcatcgcc ccggccctgg gaacggtgta cagcggcggc
     3781 cagacgatcc tgtactcggg ttccggaacg gatgctcagg acggcgcgct tcccgggagc
     3841 gctttcacct ggcaagtgga ttttcaccac gacacgcaca cgcatccctt tatcccgcct
     3901 acgatcggag cggccggcgg ctcattccag atcccgaaca ccggcgaaac ctcggccaac
     3961 gtcttctacc gcatccatct gacagtgagg gactcgggcg ggctgacgca ctcgacgttc
     4021 cgtgacatcc tcccacgcac agtgatcatc acgctcgcct cgaatccaac gggcctgcaa
     4081 ctcacccttg acggccagcc ggtcactgca ccgttcgccg tcacgggcgt cgtcgggatc
     4141 ctgcgcagca tcggggcggt cacgccgcag agactcggca agatcaagta cacattccag
     4201 acatggtcgg acggcggcgc tgcaacgcac acaatcgcga cgccggcaac gaacacgact
     4261 tacacggcga cgttcaagca gggcggaggc ccgccgcgct agggtctctg atcgccttga
     4321 ttcctcagca ggcctccatg agcgccagtg ttgcgaaggc gctaaccagg aggtaagtcg
     4381 ggcccgtgtc gaaggagaag cgggcacctg gattgtagct actgcccgcc aggttcttca
     4441 actttgtccg ccgaaatgat atcggccgct atgtaaaaag ttcgggctgt caagattttt
     4501 ggcttgactc caggaagaaa atgaggtaat tctcgatgca atagcaaaag tgtccctcgc
     4561 acgggaaatc tgggacggcg cgaggacgga ggggatcatg aaacaagggt gttggtctgc
     4621 tcgggcgggg gtcattgtgt cttgtttggc cgccagcctc atgttcatgg gctctgaagc
     4681 aactgtggct tcagctcaag tcctttacgg ttcaatcgtc gggactgtta ctgacccgtc
     4741 aaggggtatg gtcgcgaagg ccaccgtgac ggtcacgaac acggcgaccg gcttctcccg
     4801 tcaggctact acggacgacg ctggttacta ttcgattcca aacctgcctg atggtgcgta
     4861 cgatgtttcg gtcagcgcaa gcgggttcaa gcctgtcacc caaaagggcg tcgatattgt
     4921 catcaatacc gtcgcccgcg tggacgtcac cttggaggtg ggtgcggtca ctgaggccat
     4981 caccgtggag gccagcggcg ctcttcttca aacgacgaag gccgacgtca acgtgaacct
     5041 gggatcgcga gcaatcgaga atctgccgct gcccggctat cgcaactacc aaagcctcat
     5101 caatctggtt ccaggggcga caccaacgcg ataccaaaac gccgtcaccg acacgccaca
     5161 gcgcgccctg acaacgaacg tcaacggcca ggaacgcggg gcaaacaaca ctcgtgtgga
     5221 cggttcggcc gacattctgg tcactatgcc ccaccacacg gtctatgtgc ctccggtgga
     5281 aagcatcgag gaagtcaaca tctccaccaa taacttcgat tccgagcagg ggatgacagg
     5341 tggcgcagcc atcaccgtga cgacgaagtc cgggaccaac gacttccacg gcagcgcttt
     5401 cggcatgcac aacaacagcg cccttcggtc ccttctgtgg gatgagaatc gcgcgggcgt
     5461 aacgaagaaa ccaaaaggta tccgaaacat cgtggggggt agtatcggcg gacccatcaa
     5521 gaggaacaag ctgttcttct tcactgactg ggaaggtact tttgagcgtg taaatagcgc
     5581 caggctcttt tccgtcccaa cggatgatgt ccgcacgggt gacttcagca aagaactcgg
     5641 ggatcaaatt ctggatgcca gcggcaattc cattatgata cccaccaccg aaggcggtct
     5701 cgttccgctc caggaaggga tggtctttga tccctatacg ggcaacttag atggcacagg
     5761 ccgctcggtg ttttccaaca acgggcggct caacgtcatt cccacttccc ggctgaacgg
     5821 ccccatgacg agactgctgg acctggttcc tcaccccaac caagcggggg atctgaacaa
     5881 ctacttcaac tccggcacga cgcgctttaa ccgcaacaac attgacgcca aggtcaattg
     5941 gaaccggaat gataagcacc aactctggtt caagtacggc gtcatgactg ctctggttca
     6001 cggcgacttc ggcttgggcg aagcaggggg cgagtgtttg tgtcaaggtg gcggcctcgg
     6061 cgacggcaaa acgctcgtgc aaatagctgg catcgggcaa acgtataccg tctcgcccac
     6121 gttcctgatt gacgggacac tcggctggac ccgatttggt cagagtgtgg agccgccgga
     6181 cctcggcacc aactacgggt cggacgtgct gggcattcca gggaccaacg gcccggaccc
     6241 gcgggaaagc ggcctgcctg ctttttacat ggacgactac tcggcccttg gcaacccgga
     6301 aggatggaac cccctgttcc gcaacgacca gtcctacacc gtaaacgtca atgcgagctg
     6361 gatgaagggc tcccacgaga tccggtttgg cttcgacttt gtccaccatc tgatgaacca
     6421 ctggcagccg gagttgggag aagg
//
LOCUS       DATIFQ010000465         4510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_155707, whole genome shotgun sequence.
ACCESSION   DATIFQ010000465 DATIFQ010000000
VERSION     DATIFQ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155707"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VLE22_23470"
     CDS             <1..1209
                     /locus_tag="VLE22_23470"
                     /inference="COORDINATES: protein motif:HMM:NF014354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HSB17429.1"
                     /translation="MASHSEIRKTAKLMLQFLPGTDFIFSGYSSMPREDNLFGGGNFD
                     TEDMDDYGVLQRDMQVDGGVRPAREPEILAVRRRAAQGVQAVFHELGLPPVTDDEIEC
                     AVAARASVDMPARDVVADLEAAEEFLRRGRNGVDVIRALAARGFRDIAEKILTIQKLR
                     VSGDILQTSAIVLPGGRVLSAVNDPNDYNGPGTGYRLSGERQREIGNIPQALDPKTVG
                     VRESGEPILEELGDADAGDDAREIVIAVGPAFGTALGETLLGLAHAEVLGALIRGVEE
                     EGLRARVVKIRHTSDCGFIGHAGAKLSGSGIAVGIQSKGTTVIHRKDLEPLNNLELFP
                     QAPNLTLESYRVIGRNAAKYAKGEPVLPVPVQIDNMARLKYIVKTTILHLRETEQVVK
                     GGAPREVRLR"
     gene            1206..1580
                     /locus_tag="VLE22_23475"
     CDS             1206..1580
                     /locus_tag="VLE22_23475"
                     /inference="COORDINATES: protein motif:HMM:NF014355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase small subunit"
                     /protein_id="HSB17430.1"
                     /translation="MKNEYPLSEKQAERLKTPSGLPYLEITLEAVLDGRVTMADLRVT
                     ADALEMQARIAEDAGRRQLAENLRRAAELVGVPEERILEAYEALRPGRADAEALRVLA
                     DGFEREYGAARCAALLRDAAGE"
     gene            1894..2910
                     /locus_tag="VLE22_23480"
     CDS             1894..2910
                     /locus_tag="VLE22_23480"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HSB17431.1"
                     /translation="MHHTVTLIPGDGIGPEVADATVQAVEATGVPIRWERVELTREII
                     EKSGESIPDYVLESVHRTRVGLKGPVTTPVAGGFQSVNVALRKRLDLFANVRPVRTLP
                     GLKTRFQDANIDLIIFRENTEDLYAGLEHEVVTDVVTSLKVITRRASERIARYAFEFA
                     QRVGRRTITAVHKANIMKLGDGLFLRCCREVAQEYPGIEYKELIVDNAAMQLVLRPET
                     FDILVMPNLYGDIVSDLAAGLVGGLGIVPGSNMGERHAVFEAVHGSAPDIAGKGIANP
                     TALMQSAVLMLAHLGEQAASAKLQSGITRVYTEGKHLTGDVGGKATTREFTEAIVANI
                     AGSH"
     gene            complement(3157..4164)
                     /gene="gap"
                     /locus_tag="VLE22_23485"
     CDS             complement(3157..4164)
                     /gene="gap"
                     /locus_tag="VLE22_23485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HSB17432.1"
                     /translation="MAIKVGINGFGRIGRNVVRAALGNPNIDFVAVNDLTDTKTLAHL
                     LKYDSVLGPLSQEIKAGADSISVNGESMKVFATKDPNELDWSSLGAQIVIESTGKFTD
                     AKAAAAHLKGTVKKVIISAPAKNEDLTIVLGVNDGAYDPAKHNIISNASCTTNCLAPF
                     VKVLHQKFGVLKGSMTTIHSYTNDQNVLDFPHKDLRRARAAAINMIPTTTGAAKAIGL
                     VMPELKGKLDGYAMRVPTPNVSVVDLVAVLGKNTSAEEINAAFKAAAAGELKGILAYT
                     EDPVVSTDMLHNSNSSIVDGQLTKVLDGNLAKVVSWYDNEWGYSCRVVDLVEFLAKKG
                     L"
     gene            4295..>4510
                     /locus_tag="VLE22_23490"
     CDS             4295..>4510
                     /locus_tag="VLE22_23490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17433.1"
                     /translation="MPANRGNPSVVVRQRYFITFACYGARLHGEESGSVDRRHNLVGS
                     RLLEPDAQRVMAERREMLQDPYVLDEAG"
BASE COUNT          856 a         1182 c         1666 g          806 t
ORIGIN      
        1 atggcgtcgc attccgagat ccggaagacg gcgaagctga tgcttcagtt tcttccgggg
       61 acggacttca tcttctcggg ttacagctcg atgccgcggg aggacaacct gttcggcggc
      121 ggcaacttcg acaccgaaga catggacgat tacggcgtgt tgcagcgcga tatgcaagtg
      181 gacggcggtg tgcgcccggc gcgtgagccg gagattctgg cggtgcggcg gcgcgcggca
      241 caaggggtac aggcggtgtt tcacgagctg gggctgccgc cggtgaccga tgacgagatc
      301 gaatgcgcgg tggcggcgcg ggcaagcgtg gacatgcccg cgcgggacgt ggtggccgat
      361 ctcgaggcgg ctgaggagtt cctgaggcgc gggcggaacg gcgtggacgt aatccgggcg
      421 ctggcggcga gggggttccg ggacatcgcg gagaagatcc tgacgattca gaagctgcgt
      481 gtgtcggggg atatcctgca aacgtcggcg atcgtgctgc cgggcgggcg ggtgttgagc
      541 gcggtgaacg atccgaacga ttacaatggg ccgggcacgg gctaccggct gagcggggag
      601 cgtcagcggg agatcggcaa cattccgcag gcgttggacc cgaagacggt cggggtgagg
      661 gagtcgggag agccgattct ggaggaactt ggcgacgcgg acgcgggcga cgatgcgcgg
      721 gagattgtga tcgctgtcgg gccggcgttc gggacggcgc tcggagagac gctgctcggg
      781 ctggcgcacg cggaggtgct gggtgcgctg atccgcggcg tggaggaaga agggctgcgg
      841 gcgcgggtgg tgaagatccg gcacacgtcg gactgtggct ttatcgggca cgcaggggcg
      901 aagctgagcg ggtcggggat cgcggtgggg attcagtcca aggggaccac ggtgattcac
      961 cggaaggacc tggagccatt gaacaacctg gaactgttcc cgcaggcgcc gaatctgacg
     1021 ctggagtctt accgggtgat cgggcgaaac gcagcgaagt atgccaaagg cgagccggtg
     1081 ctgccggtgc cggttcagat cgacaacatg gcgcggctga agtacatcgt gaagacgacg
     1141 attctgcacc tgcgggagac ggagcaggtg gtgaagggcg gcgctccgag ggaggtgagg
     1201 ctgcgatgaa gaatgagtat ccactttcgg agaagcaagc ggagcggctg aagacgcctt
     1261 cgggtcttcc gtatctggag atcacgctgg aggcggtgct ggatgggcgc gtgacgatgg
     1321 cggacctgcg ggtgacggcg gacgcgctgg agatgcaggc gcggatcgcg gaggatgcag
     1381 gacgacggca actcgcggag aacctgcggc gggcggcgga actggtggga gtgccggagg
     1441 aacgaattct cgaggcctac gaggcgctgc gtcccgggcg ggcggatgcg gaggcgctga
     1501 gggtgttggc ggatggcttt gagcgggagt atggggcggc gcggtgcgcg gcgctgttgc
     1561 gggacgcggc gggagagtag gagccgtaag tcgttgggaa gacggcaccc aagtgtgagc
     1621 ggtacgtttg gtactataga aggtacgtct acattacccc gcgttggagg agagcgatca
     1681 ccccataaaa agacagtgga aacggtagta tgcggagttt aactccggcc gaaattcgat
     1741 tatatttccg actgggaggc tgcggattcg cgctagaggt ggacgtgggg ttgccgccga
     1801 aagacacgca gcccgcggct tccccgagcc aatgcggagt gacgggaccg ggcagaggcc
     1861 gatggattcc ggggatgcgg ctataatgcc tctgtgcacc acacagttac tctgattcca
     1921 ggagacggta ttggtccgga ggttgctgac gccacggttc aagcggtcga ggccactggg
     1981 gtacctatcc ggtgggaacg ggtcgaacta acccgggaaa tcatcgagaa atccggcgaa
     2041 tcgattccgg actatgttct ggagtccgta caccgcacgc gagtgggatt gaaaggccca
     2101 gttacgacac cggttgcggg aggtttccag agcgtcaacg tggctctccg caagaggctt
     2161 gatctgttcg ccaacgttcg ccccgtgcgc acgctacccg ggctcaagac gaggtttcag
     2221 gacgcgaaca tcgacctgat tatcttccgc gagaacacgg aagacctgta cgccggactg
     2281 gaacatgagg tggttacgga tgtggtgacc agcctgaagg tgatcacccg ccgcgcctcc
     2341 gagcgaattg cccggtacgc attcgagttc gcgcagcggg tgggccggcg gacaattacc
     2401 gccgttcaca aggcgaacat catgaaactc ggcgatgggt tgttcctgcg gtgctgccgc
     2461 gaggtggcgc aggaataccc gggcatcgag tacaaagaac tgatcgtcga taacgccgcg
     2521 atgcagctcg tgctgcggcc ggagacattc gacattctgg tgatgccgaa cctgtacggc
     2581 gacatcgtgt cggacctcgc ggcggggctg gtaggcgggc tagggattgt ccccggctcg
     2641 aacatgggcg agcggcacgc agtattcgag gcggtgcacg gcagcgcgcc ggacatcgca
     2701 gggaagggga tcgccaatcc gacggcgctc atgcagtcgg cggtgctgat gctggcgcac
     2761 ctcggcgagc aggcggcctc ggcgaaacta cagtcgggaa tcacccgcgt ctatacggag
     2821 ggtaagcacc tcacggggga cgtgggcgga aaggcgacga cgagggagtt caccgaggcg
     2881 attgtggcga acatcgcggg cagccactag cccgcattac tcaccagcga tctactgcgg
     2941 cgcgcgatag ggccgaaact gcggcgttgc gctcggtcgg agtcctcgac gtactgtcaa
     3001 gtacgcctgc gggctcctcc cgtcgcgcgc cttgcatttc cggccctctc ccgcgcctcg
     3061 tccacgacgg tggtgagaaa tgcgggctag accggggaac ggatcgctcg caggcgagcc
     3121 gccgccgaac ggcggcggct cagcgcgcgg gaaaaactac agacccttct tcgccagaaa
     3181 ctcgacgagg tcgacgacac ggcaggaata gccccactcg ttgtcgtacc aggagacgac
     3241 cttcgcgagg ttgccgtcga gcaccttggt caactggccg tccacgatcg agctgttgga
     3301 gttgtgcagc atgtcggtgg aaacgaccgg gtcctcggtg taagcgagaa tgcccttcag
     3361 ttctccggca gcggcggcct tgaacgcggc gttgatttcc tcggcggagg tattcttgcc
     3421 gaggacggcc acgaggtcaa cgaccgacac gttcggggtc ggaacgcgca tggcgtaccc
     3481 gtcgagcttg cccttcagtt ccggcatcac gaggccgatc gccttggcgg cgccggtggt
     3541 ggtggggatc atgttgatgg cggcggcacg ggcgcggcgg aggtccttgt gcgggaagtc
     3601 gaggacgttc tggtcgttgg tgtagctgtg aatggtggtc atcgacccct tgagaacgcc
     3661 gaacttctgg tgcagcacct tcacgaaggg cgccaggcag ttggtggtgc aggaagcgtt
     3721 agagatgatg ttgtgcttgg ccggatcgta ggcgccgtcg ttgacgccga ggacgatggt
     3781 gaggtcctcg ttcttggcgg gagcggagat gatgactttt ttcaccgtgc ccttgaggtg
     3841 agcggcggcg gctttggcgt ccgtgaactt tccggtggac tcgatgacga tctgagcgcc
     3901 gagagagctc cagtcgagct cgttcgggtc cttggtggcg aagaccttca tcgactcgcc
     3961 attgacggag atgctgtcgg cgccggcctt gatctcttgc gacagggggc cgagaacgga
     4021 gtcgtacttc aacaggtgcg ccagcgtctt ggtgtcggtg agatcattga ccgcgacgaa
     4081 gtcgatgttc gggttgccga gggccgcgcg gacgacgttc cgtccgatgc ggccgaagcc
     4141 gtttatacca actttgattg ccatagattt agattctcct gtggggtagt gcgggaacag
     4201 aaaacttaac tttccatgat atcaaaaggt gcaggctgcg aatggacgcg aataggcgcg
     4261 gggtgcacga catgaaaggg ataaattctt agcaatgccg gcgaatcgag gaaatccgag
     4321 cgtagtggta cgccagaggt atttcattac gttcgcctgc tacggcgcgc gcttgcacgg
     4381 cgaggaatcg ggatcggtgg atcggcgtca caatttggtt ggcagccggc tgttagagcc
     4441 cgatgcacaa cgcgtcatgg cagaacgtcg agaaatgctc caggatccct atgtgctcga
     4501 cgaagcgggc
//
LOCUS       DATIFQ010000466        18135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_733007, whole genome shotgun sequence.
ACCESSION   DATIFQ010000466 DATIFQ010000000
VERSION     DATIFQ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 18135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18135
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733007"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="VLE22_23495"
     CDS             complement(<1..1396)
                     /locus_tag="VLE22_23495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB17434.1"
                     /translation="MPQAFYVLFGAVFTASTALALGKLLLRQLGLKFHRVEEHVFAFA
                     TGAACLSLLVFLLTAAQLARKGVFLALGLLVIGMAIRRGAHRPLGESFPPLPRLWRIL
                     FGAIFVFFTVVYFANAMAPEMSPDGSSYHLGLVARYMRERGFTRITTNMYANLSQGVE
                     MLFLYAFAFGRHSAAALVHFSFLFTLPLAMLAYAKRFGFPKAGAAAALLFYLSPVVGI
                     DGISAYIDVATACVLFCLFYLLQIWDSERNPALLVPIGLLAGFGYAAKYTAFVAVPYA
                     VVFVGWKLLRARRPCRKPMLVIVCCALLMMVPWIAKNWIILGNPVSPFFNRLFPNPYV
                     HVSFEKEYAQAMRNYPGLKSRWDIPLEVTLRGNVLHGLLGPVFLLAPIGLLALREPQA
                     RQLLLAAAVFGSTYFANIGTRFLIPSLPYIALLMAMAMARWRVVMPVLVVAQALASWP
                     PNIKKYSDDRAWRLV"
     gene            complement(1396..1905)
                     /locus_tag="VLE22_23500"
     CDS             complement(1396..1905)
                     /locus_tag="VLE22_23500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17435.1"
                     /translation="MASLLWLFGCARIPDIYAPPIERKPLTGPEQFALGPLVRTSDSN
                     ADAHIVRDISRTVEGGGWRWAHQRPELRFRLQTTENLKFVMDFGVPETTYRDTGPLKV
                     SVFVNGQLLDVIPVDKPGDRHFEKPVPGSWLRTDADTKVVAEVDKPWVAPADGAKLGF
                     TLSRAGFVQ"
     gene            complement(2003..2683)
                     /gene="nth"
                     /locus_tag="VLE22_23505"
     CDS             complement(2003..2683)
                     /gene="nth"
                     /locus_tag="VLE22_23505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSB17436.1"
                     /translation="MPAAARTKAKRPKTAAERKTRIVRIIDALDRLYPAATCALHHHN
                     AWELLVATILSAQCTDKRVNEVTPGLFRKYPTIKDFASVSQDELAQDIRSTGFFNNKA
                     KSIIGAARKILSDFGGKVPKTMEEALTIPGAARKTANVVLGTAYGIASGVVVDTHVQR
                     IAQRLDLSKEKDPGKIEQDLMRVIPQERWILFSHQMIHHGRNLCVARKPRCAECPIDP
                     LCYAKDKT"
     gene            2732..3928
                     /locus_tag="VLE22_23510"
     CDS             2732..3928
                     /locus_tag="VLE22_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17437.1"
                     /translation="MRDSLLLALAVLAVFGLSLTDTFHFDDYAIFSDPAITSNSGWLD
                     VWRPWQIRPLTWLTFWLNHRLGGREPAGYHAVNLILHLAAVLLLWDLLPRIIPRRAAL
                     IASMVFALHPLVSEPVLNVLARGTLLATLLCLMSLRSWVVGRRWIAVLWFGLALLANE
                     ECVPFPIFLLLLHFSISRNAAEFRQIATMLGLSLVAGLRVLFATVIDPGLGAGPQAGV
                     PVSTYLAGQGVVILRYFRLFVFPWGFTAGPEFQVPSAWVAVGAWLVVLALAIAAAWRF
                     SHAREGFWFLGGLVLLLPSSSIFPAADLAADRRMYLPMIAFAAVAGLALRAVKTRYVL
                     VLAALLGLLSFGRAKAWRDDRSLWLEAVRSAPARSRQVTRRYWQTTAVPQAVTISIPP
                     LAPRFS"
     gene            complement(3856..4176)
                     /locus_tag="VLE22_23515"
     CDS             complement(3856..4176)
                     /locus_tag="VLE22_23515"
                     /inference="COORDINATES: protein motif:HMM:NF002776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HSB17438.1"
                     /translation="MLLVTTHSIEGKQIVKYLGLVSGEAILGANVFKDLFAGIRDIVG
                     GRSAAYEQELRKAKDIALNEMTEQARQMGANAVIGIDLDYENLGASGGMLMVTACGTA
                     VVCQ"
     gene            complement(4221..4856)
                     /locus_tag="VLE22_23520"
     CDS             complement(4221..4856)
                     /locus_tag="VLE22_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17439.1"
                     /translation="MFTWICPKCGREVPPSYNECPDCAREAPVAQQPPAEPPLALPPT
                     KPRVNTEAKHRMPGWLLAVIFAAGFIVVGAVVFFASRYFLGTRDNKVAAEIESAPMGL
                     DAPPKPSGYSKYIEVTGFRLTEDKTQKLEVQFLVVNHSAAEIASLSGEVTLKPKTGKA
                     DAKPLAKFSFKIPSLRPYQSQELKASAQTGLRAYELPDWQFLEADVLITSP"
     gene            complement(4869..5960)
                     /locus_tag="VLE22_23525"
     CDS             complement(4869..5960)
                     /locus_tag="VLE22_23525"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17440.1"
                     /translation="MQKKLRIGMVGAGFMGRAHSNAYRQVRHFFDVPFELECRAVCAR
                     NRENLTKLAARWGWAETTTDWEALIDRPDIDVVDIAAPNAMHAPVAIRAAEAGKIVWC
                     EKPLSTNLAEAERIVAAARGVPSMVWYNYRRVPAISFAKRLVDDGRIGRAFHYRATYL
                     QESGNDPTRPPSWKTIREHAGSGVLGDLLSHVVDSSLWLNGAIREVSGMMQTFADGRD
                     VDDATLALVRFANGSVGTFEATRYATGCRNRNGFEMHGQLGMLRFNLEDMNHIEFFDA
                     TEPRNLQAPRRLLVTGPDHPYAANFWKPGHIIGYEHTFIAALGDFLGALAEGRPFHPD
                     FEDGLHVEKVLDAVQRSARTGRWMEVEQA"
     gene            6025..6627
                     /locus_tag="VLE22_23530"
     CDS             6025..6627
                     /locus_tag="VLE22_23530"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HSB17441.1"
                     /translation="MKFYRRARGRLRSLGILPGTFNPPTRAHLALAHATLEFVDEVVF
                     VLPAVLPHKSYGKVTLEDRLRMLKAALRGERRFSIASTAGGLLIEIARECRDAYGPEV
                     SLKFLCGRDAAERFVNWDYGRPGAFLEQLREFELLVAPRGGNYEPPAEMRGRIHMLPE
                     FTRQDEVSGTEVRKRIKQGLAWEHLVPSRIVAMVRELYGG"
     gene            complement(6640..8322)
                     /gene="rpoD"
                     /locus_tag="VLE22_23535"
     CDS             complement(6640..8322)
                     /gene="rpoD"
                     /locus_tag="VLE22_23535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HSB17442.1"
                     /translation="MIEEKYGQLKRLMDTGKEKGFVLYDEVNELLPDEMVGGAELDQL
                     LADLDSAGVEILEEPRLDFEKKIEEGEELAELEISTELSEKTNDPVRMYLREMGTVPL
                     LTREGEIELARRIERGQRSVLRALSRSALVIREVLHIGEELDRDTLQLRDLLILPDMV
                     VSDDSLAKQKSELHAQIAEIGKQYKKSQQFRQKLQAISRGMKPKQHRTLRFGLARSLV
                     TVSRISRQIQFSPLALNRFTDLVRQAVEDFRPVERTIARIQRRLEEHGHEGAAVVREL
                     RKELRHATQRLQQLEEDCGASALDLRRTLQIIERGELETETAKKQLIEANLRLVVSIA
                     KRYTNRGLQFLDLIQEGNIGLMKAVDKFDYRRGYKFSTYATWWVRQAITRAIADQART
                     IRIPVHMIETINKLVRTQRELQQELRREPTTEELARKMEMSAGKVRKVLRIAQEPISL
                     ETPVGEEEESHLGDFIVDRQGISPSEAVINLNLREQTAEVLKTLSPREEKIVKMRFGL
                     MDGSEHTLEEVGQYFAVTRERIRQIEAKALRKLRHPSRSHRLRAFLEQGLRD"
     gene            complement(8397..10136)
                     /gene="dnaG"
                     /locus_tag="VLE22_23540"
     CDS             complement(8397..10136)
                     /gene="dnaG"
                     /locus_tag="VLE22_23540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HSB17443.1"
                     /translation="MDFVEQLKSSVDIVKVIGEYVRLRRVGAGPRYMGLCPFHTEKTP
                     SFSVHQAHQFYKCFGCGVGGDVLKFVMEVERLSFYEALKLVADRNGIPMPKRAEYADE
                     DSKLRASLYQMHEIAARAFQTNLGGAPGAEARQYLGRRGVPPSLAEEFGIGLSERSGQ
                     DLVRRFEREGFSAEQMEASGLLLRRQEGGGFFDRFRGRLMFPIHNESGKIIGFGGRAL
                     AANDEPKYLNSPETPIYRKSYVLYNLHRAKETIRKLDRTILVEGYMDVVGVFGAGVHE
                     VVASCGTALTSTQVRALKRHSERIVVNFDPDTAGASAAERSIQMLLEEGMHVRVLHLE
                     GGLDPDEYVKEHGADAYRSRMEKAPGYFYWLADRARARFDMRNAEGRVAALQFLLPAI
                     QRMPDKLERAAIASDVATYLGVEQGLVLEHFRKSAANRSDRPMQVDREPIRAVEQLLL
                     NALLANRDIRGEIVPALSQIPQLEKSPASRIFRTLFTLHESRPEFGFSELEARLDDGD
                     KALLASVVFADELGEGSYTREQALACVRALEVEAREGKRAELRSRVKEAEREGRMEEA
                     LRLAEELSRAERV"
     gene            complement(10115..12250)
                     /locus_tag="VLE22_23545"
     CDS             complement(10115..12250)
                     /locus_tag="VLE22_23545"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HSB17444.1"
                     /translation="MTRRLALRVVPSAVLLVEVLVFYRQVLLDSRYVIPWDLRYYHLP
                     QIEFVARAFRNGGLPLWDPYTYCGMPIYANLTTQLFYPPTLVTVWLSNLAGGGNLLYF
                     LEWQLVLHVFLGGVLAYLLLRRMGTSVTAALFGGTVFQLGGYFASQTQHLGAMDAAAW
                     MPLAWLSAVSLSRGFSWRWTAALAASLSMAILAGFPAVTFVVAASTMIVGLLAGWRKT
                     VVPMALAAVWSLLLAAVQVLPTAELTRLSVAKYRSDWIGDGGGLPLASLVSLVAPNYY
                     GIFDLSTYAHPWQPTFLYLFCGISGLVLALAAVIFRRDRQVRLFSIALVISILWMLGA
                     STPVGKVAFRVLPNIMKSSVYPEFAMAAFVLGVAVLAGLGADRFAIFRKPLAGGLLVA
                     VAAVELTAAGSGRNLNTGSLATEPGLTYSSFDGSTEVLARVRYLTGRSKPPWRIDTID
                     DSMNWAHGAALTEVPTANGNDPFALIRFMQVRLTFTGGERWGRYYQVLKPESRVLDLM
                     NVRYLLSRTPVGETRDRGLVPVAEIPGRTVYENRDALPRFFLVDRIQHAGNMEEALSE
                     LRSAEFRPAEVAVVEEPVEFPGSAGQPGGAIRLIKYEPRSITLEVESAAPAFLVTSET
                     YYPGWRAFVDGRERPVVLTNVAFRGMPVDAGRHRIEMRFEPAILWRGATLSAAGWALL
                     FPAFVFGDNRRKRTAVSGAATSGWTSLSN"
     gene            complement(12301..12819)
                     /locus_tag="VLE22_23550"
     CDS             complement(12301..12819)
                     /locus_tag="VLE22_23550"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HSB17445.1"
                     /translation="MSILKFLGLETEPPSGTPETETVRKITDALDRMDFDQARYIAAF
                     AYVLARAARADLHISELETRAMERIVVEHGGLPEEQAILVVQIAKTQNKLFGATEDFV
                     VTREFGSIATREQKLTILDCLFSVAAAEGLISMEEDNVIRQIASELKLDHRDFIALRS
                     KHRDHLSILRKR"
     gene            complement(12874..14178)
                     /locus_tag="VLE22_23555"
     CDS             complement(12874..14178)
                     /locus_tag="VLE22_23555"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17446.1"
                     /translation="MSDTTRRSFLGRSATALGAAPAFIRSFATAAPSDRIRVGLIGCG
                     GISGADLPTFFLNPEVDCPIVCDIDDERIAKRASLVEKHRQTTPDTVKDFRRVIDRKD
                     IDVCLICTPDHWHALPAIYACQAGKDVYVEKPLATSIGEGRAMVTATRNHKRIVQMGT
                     QWRSGPHYQEAVDFVRSGKLGRIRQVRAWAYLDWVGGIGKPADSDPPPSVDYNMWLGP
                     APERPFNKNRFHFNFRWFWDYAGGLMTDWGVHLINIALWAMGPEPPRSVTSSGGKFVV
                     DDNSETPDTQIAVYDFSSYTLVWEHQLLGGIGPGGRPHGLSFSGTEGTLILDADGWEI
                     HAEPKKKTLGEWTRKVNPQDRTADGRPAHVRNFLDCVRSRQDPVENVELGHFVSTVAH
                     LGNVALRTGRKIEWDAATERATGLPEADRFITYPYRKPWSLA"
     gene            complement(14328..15236)
                     /locus_tag="VLE22_23560"
     CDS             complement(14328..15236)
                     /locus_tag="VLE22_23560"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB17447.1"
                     /translation="MQKITPHLWFDKEAREAAELYVSLFPGSRITNVTRLSGTPSGDC
                     DLVSFELAGQPFMAIGAGPLFKFNPSVSFHAKCGTKEEVDAIWEKLSPGGKVLMPLDS
                     YPFSERYGWLEDRYGLSWQVILAGGMEIKQRFTPVLMFVGKVCGKAEEAISFYASVFR
                     NAPNGAEVGETKATVFARYGKGEDPDTEGTVRYAHLSLLGQEFGAMDSARAHQFAFNE
                     AISFIVPCDSQEEIDYFWGKLSADPRAEQCGWLKDRYGVSWQITPAVMRELLGGEDRE
                     RTARLTQAFLKMKKFDIAALKRAAAG"
     gene            15662..16822
                     /locus_tag="VLE22_23565"
     CDS             15662..16822
                     /locus_tag="VLE22_23565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17448.1"
                     /translation="MTAVAVLTLALGIAATTAVFGWIDGMVLHPLRGAADDGQLAVLE
                     AVRASGIEDPNVSYADCRDFQDSLRSISGLVLNQQAPASVGDGENAYSAWFELVSGNY
                     FDVLGVKPVLGRTFAREEYGDRAKAFTAVISYRMWRHYFQNDPSILGRTVHINRHRVT
                     IVGVAPPEFHGDIPGIAFDGWVPAPLTGERDRDARHFRAIVRLKPGLSVSEANAEVAT
                     VAARLARAFPTTNEGIGARIVPIWKAQSGLSGILASPLAILGAACGLVLLIACANVAN
                     LLLARSAARQTEFGIRAALGAGPGRLSRQLVCESLLLAALATLVGLPLALWSQDILVY
                     LVPPTGLPLYFNVHPSARQFLFAALVCLASALISGLPPAFQSVRRSLVDALK"
     gene            17073..18032
                     /locus_tag="VLE22_23570"
     CDS             17073..18032
                     /locus_tag="VLE22_23570"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSB17449.1"
                     /translation="MPCQGCQAPLYSDSIPLGFGLGKWTEVMVEGYAARPGENLDVHH
                     ASVSSGYFDLLRVPLLAGRDFRPEDNEKAPRVMIVNESFARRFFDGRDPVGHRVHIYG
                     IPFTIVGMVKDSKYCSLSEAPQPYFYMSFDQVHNGSGEGGVALYARTDGDARGFVPVL
                     RREMSAIDPNSAGLTAMPLSDYISAAWFAPRLASLLLGVLGVISMLLAGVGLYGVMAY
                     SVSQRTREIGIRMALGADPGGVLRTVMRRGLLLALSGIAAGLAISLAATPQIEPLLYR
                     VSPADPVSIAGAALFLIAVAVVASIIPALRATRVDPMVALRQE"
BASE COUNT         3286 a         5787 c         5475 g         3587 t
ORIGIN      
        1 tgacgagacg ccatgcccgg tcgtccgagt acttcttgat gttcggcggc cacgaggcca
       61 gtgcttgcgc cactaccaga accggcatca cgacgcgcca gcgcgccatg gccatcgcca
      121 tcaacagcgc gatgtacggc agtgatggaa tcagaaaacg ggtcccgatg ttggcgaaat
      181 aggtggagcc gaagacggcg gctgcgagca gaagctgccg cgcttgcggc tcccgcaacg
      241 ccagaagccc gatcggagcc agcagaaaca ccggcccgag tagcccatgc agcacattgc
      301 cccgcaacgt cacttcgaga ggaatgtccc accggctctt caatcccggg tagttccgca
      361 tcgcctgggc gtattccttc tcgaaggaga cgtgaacgta cgggttcgga aacaggcgat
      421 tgaagaacgg cgacaccgga ttgcccaaga ttatccagtt cttggcaatc caagggacca
      481 tcatcagcag cgcgcagcag acgattacaa gcatcggctt gcggcagggg cgtcgtgcgc
      541 gcaggagctt ccatcctacg aagaccacgg cataagggac ggctacgaag gctgtatact
      601 tcgctgcgta tccgaagccg gccaaaaggc cgatgggtac gagcagcgcc gggttccgct
      661 cgctatccca gatctgcagg aggtagaaga ggcaaaacag aacgcaagcc gtcgcgacgt
      721 cgatataggc actaattccg tcgatgccta caaccgggct cagatagaac aacagggccg
      781 cggccgctcc cgctttcgga aacccgaagc gcttcgcgta cgccagcatc gccaggggca
      841 gcgtgaaaag gaacgagaaa tgcaccagcg ccgccgccga gtgccttccg aacgcgaagg
      901 cgtacaggaa cagcatttcg acgccctgcg ataagttggc gtacatgttg gtggtgatcc
      961 gcgtgaatcc ccgctcccgc atgtacctcg ctaccaagcc cagatggtac gagcttccat
     1021 ccgggctcat ctcgggcgcc atggcgttgg cgaagtagac cactgtgaaa aaaacaaaga
     1081 ttgcaccgaa gaggattctc cagaggcgag gcaggggagg gaacgactcg ccgagaggcc
     1141 ggtgcgcacc gcgccggata gccatcccga tcaccagcag gccgagagcc aggaacactc
     1201 cctttcgcgc gagctgcgcg gccgtgagca ggaacacgag aaggctcaaa cacgccgcgc
     1261 cggtcgcgaa ggcgaaaacg tgttcctcca cacggtggaa cttcaggccc agttggcgaa
     1321 gcagaagttt accaagtgcc agcgcggtgg acgcggtgaa gacggcgcca aagagaacgt
     1381 agaaggcctg cggcattact ggacgaagcc ggcgcgcgac agggtgaatc cgagcttcgc
     1441 gccgtcggcc ggcgcgaccc aaggcttgtc aacctcagcg actaccttcg tgtctgcgtc
     1501 ggttcggagc catgatccgg gcaccggctt ttcaaagtgc cggtcaccgg gcttatcaac
     1561 cgggatgacg tcgagaagct ggccgttgac gaagaccgag actttgagcg gaccggtatc
     1621 ccgataggtt gtctcgggta ccccgaaatc catcacaaac ttcaggttct ccgtcgtttg
     1681 cagccggaac cggagttcgg gcctttgatg tgcccagcgc cagcccccac cctccactgt
     1741 cctgctgatg tcgcggacaa tgtgagcgtc tgcgttcgaa tcgctcgtgc ggacaagcgg
     1801 gccaagagcg aattgttcgg gtccggtcag cggcttgcgc tcgatgggcg gcgcgtagat
     1861 gtccggaatg cgcgcacaac caaacagcca gaggagcgat gccacgacca gccacttcgc
     1921 acaggctgaa gagggtgctg ctgaaaaacc ggacggcatt tcgctataga ttccgttccc
     1981 tccggtcgcg gctcggtaag gattacgtct tgtccttcgc atagcacagc gggtcgatcg
     2041 ggcactccgc acagcggggt ttccgggcca cacacaagtt ccggccgtgg tggatcatct
     2101 ggtgagagaa gaggatccac ctttcctgcg ggatgaccct cataaggtcc tgctcgattt
     2161 ttcccggatc cttctccttt gacagatcca gccgctgggc aattcgctga acgtgggtat
     2221 ctactacgac gccggatgcg ataccgtacg ccgtgccgag taccacgttc gctgtcttcc
     2281 gtgcagcgcc cgggatggtg agcgcctcct ccatcgtctt cgggaccttg ccgccgaaat
     2341 cactgagaat cttgcgggcg gcgccgataa tcgacttcgc cttgttgttg aaaaacccgg
     2401 tcgagcggat gtcctgagcc agctcgtcct ggctgacgga tgcgaaatcc ttgatggtgg
     2461 ggtacttccg aaacagcccc ggcgttacct catttacgcg cttgtccgtg cattgcgccg
     2521 acaggatggt agccaccagc agctcccatg cgttgtggtg gtgaagcgcg caagtggcgg
     2581 cgggatagag ccggtcaagc gcatcgatga tccgcacgat gcgggtcttc cgttcggccg
     2641 ccgtcttggg tcgtttggcc ttcgttcgcg ccgcggctgg catgtgtgaa acctccggcc
     2701 cctcgggctg aattgatcta acatagtaac attgcgtgac tcgctgctcc tcgcgctggc
     2761 ggtcctggcc gtgttcggac tcagcctcac cgacacgttt cattttgacg actacgcgat
     2821 cttctccgac cccgcaatta catcgaactc gggctggctc gacgtctggc ggccgtggca
     2881 gatccgcccg ctgacatggt taaccttctg gttgaatcac cgcctcggcg gacgcgagcc
     2941 cgccgggtac cacgcggtga atctgattct gcacctggcc gcggtgctgc ttctctggga
     3001 tcttctaccg cggatcatcc cacggcgggc ggcgctcatc gcgtccatgg tcttcgcgct
     3061 gcacccgctg gtgagcgaac cggtcctcaa cgttcttgcg cgcggtacgt tgctggcgac
     3121 gctgctctgc ctgatgtcgt tacgctcgtg ggtggtagga cggcgctgga tcgcggtgct
     3181 gtggttcggc ctcgcgctgt tagccaacga ggagtgcgtg ccgttcccaa tcttcttgct
     3241 cctcctgcac ttctcgattt cgcggaacgc cgccgagttc cgtcagatcg cgacgatgct
     3301 cggcctgtct ctggtggccg ggctgcgagt cctgttcgct acggtgatcg atcccggctt
     3361 gggtgcgggg ccgcaagccg gcgtgccggt gtcaacatat ctggcgggtc agggagtggt
     3421 gatcctacgc tattttcggc tgtttgtctt tccgtgggga tttaccgccg gccccgaatt
     3481 tcaagttccc tccgcctggg ttgccgtcgg cgcctggctg gtggttctgg cgctcgccat
     3541 cgcggcagcc tggcgattct cacacgcccg agaggggttc tggtttcttg gtgggctcgt
     3601 gttgttactg ccgagttcgt cgatctttcc cgcggccgac ctcgccgccg accggcggat
     3661 gtatctgccg atgattgcgt tcgccgccgt ggccggcctg gcgttgcgcg ccgtcaagac
     3721 tcgatacgtg ctggtgctcg ccgcgctcct agggcttttg agttttggac gcgcgaaagc
     3781 gtggcgcgac gatcggtcgc tctggctgga ggctgtgcgg agtgcacccg ctcgcagccg
     3841 ccaagtaacc cggcgttact ggcagaccac cgcggtaccg caggcggtaa ccatcagcat
     3901 cccgccgctg gcgccaagat tctcataatc gagatcaata ccgatgaccg cgttggcgcc
     3961 catctgccgc gcctgttcgg tcatctcgtt cagagcgatg tccttcgcct tccgcagttc
     4021 ctgttcatat gcagccgagc ggccgcctac gatgtcgcgg atgccggcaa aaagatcctt
     4081 gaaaacgttg gcgcccaaaa tggcttcgcc gctcaccaat ccaaggtact tcacaatctg
     4141 ttttccttcg atgctgtgcg tcgtcaccag tagcattgtc tctcctgact cgctgagttt
     4201 ccgggcgata ctcccgccac ttatggcgac gtgatcagca cgtcggcctc aaggaactgc
     4261 cagtcgggaa gttcatacgc acgaagcccg gtttgagccg atgccttcaa ctcctgtgac
     4321 tggtacggcc gcagcgacgg aatcttgaag gagaacttcg ccagcggctt ggcgtcggcc
     4381 ttgccggtct ttggtttcag ggttacttcg ccggaaaggc tcgcaatctc cgccgccgag
     4441 tgattcacca ccagaaactg aacctccagc ttctgtgtct tgtcttcggt cagccggaaa
     4501 ccggtgactt cgatgtactt cgagtagcca cttggtttcg gtggcgcgtc caggcccatt
     4561 ggcgccgatt cgatttctgc ggccaccttg ttgtccctcg tccccaggaa gtatctcgac
     4621 gcgaaaaaca caacggcgcc gaccactatg aaccccgcgg cgaagatcac cgccagcagc
     4681 caccctggca ttcggtgctt tgcttccgtg ttgactctcg gtttggtggg gggcaatgcc
     4741 agcggaggct cagccggggg ctgttgcgcg acaggagcct cccgcgcaca gtcgggacac
     4801 tcgttgtaag acggggggac ctcacggccg cacttcggac agatccaagt gaacattccc
     4861 tttctattct acgcctgctc gacctccatc cagcggcccg tccgcgccga ccgctggaca
     4921 gcgtccagca ctttctcgac gtggaggccg tcttcgaagt ccgggtgaaa cgggcgtcct
     4981 tcagccagcg cgcccagaaa atcgccgagg gccgcgataa atgtgtgctc gtacccgatg
     5041 atgtgacccg gtttccagaa gttagccgcg tagggatgat cgggtcccgt cacgagcagc
     5101 cgccgcggcg cctggagatt ccgaggctcc gtcgcgtcga agaattcgat gtggttcatg
     5161 tcttccaggt tgaagcgcag cattccgagt tgcccatgca tctcgaagcc gttgcggttg
     5221 cggcagccag tggcgtatcg tgtagcctcg aaagtgccga cgctgccgtt cgcaaaacgc
     5281 acgagtgcca gcgtagcgtc gtccacgtcg cggccgtcgg cgaaggtttg catcattccg
     5341 gacacctctc ggatagcgcc gtttagccag agagagctgt caaccacatg gctgaggaga
     5401 tcgccgagca caccggaacc ggcgtgttcc cggatggtct tccagctcgg cggccgcgtg
     5461 ggatcgttgc ccgactcctg taggtacgtc gcgcgatagt ggaaggcgcg tccgatgcgt
     5521 ccgtcatcca ccagtctttt cgcaaaggag atcgcaggta cgcggcggta gttgtaccat
     5581 accatcgatg gaacgcctcg ggcagcggcc acaatccgct cggcttcagc gaggttcgtc
     5641 gacaaaggct tctcgcacca gacgatctta cccgcctccg cagcccggat cgcaaccggc
     5701 gcatgcattg cgttcggcgc cgcgatatca accacgtcga tgtccggccg gtcgatgagc
     5761 gcctcccaat ccgtcgtggt ctccgcccaa ccccacctgg ccgccagctt cgttaggttc
     5821 tcccggttcc gggcgcagac ggcacggcat tcgagctcaa aaggaacgtc aaagaagtgc
     5881 cgcacctgcc ggtacgcatt ggagtgggct cgccccatga atcccgcgcc taccataccg
     5941 atgcggagct ttttttgcac caagctgtta ctcctcccgc agtcattgtc gccgatcaag
     6001 cgtctcgtgc gttgtaatgc tgctatgaag ttctatcgcc gcgccagggg ccgtctacgg
     6061 agcctcggca ttctgccggg aacgttcaac ccgcccacgc gtgcacatct cgcgctggcg
     6121 catgcaaccc tcgagttcgt cgatgaagtg gtgttcgtcc tgcctgccgt gctgccccac
     6181 aagtcgtacg ggaaggtcac gcttgaagac cggcttcgga tgctgaaagc cgcgttgcgc
     6241 ggcgagcggc gcttctcgat cgcttccacc gcgggcggct tgctcatcga gattgcccgc
     6301 gagtgccggg acgcatacgg gcccgaggtt tcgctgaaat tcctgtgcgg ccgcgatgcc
     6361 gccgagcggt tcgtgaactg ggattatggg cggcccgggg cgtttctcga acagctccgc
     6421 gaattcgagt tgctggtagc tcctcgcggc gggaactacg agccgcccgc ggaaatgcgc
     6481 ggccgtatcc acatgttgcc cgaattcacg cgacaggatg aggtgtccgg cactgaagtt
     6541 cgcaaacgca tcaagcaagg ccttgcctgg gaacatctcg tcccatcacg gattgtcgct
     6601 atggtgcggg aactctacgg cggttgatcg tcgttcgagc tagtcgcgaa gcccctgctc
     6661 caggaacgct ctcaaccgat ggctacggct ggggtggcgg agtttgcgaa gcgcctttgc
     6721 ctcgatctgg cggatgcgct cgcgggttac cgcgaagtac tgtccgactt cttccaacgt
     6781 gtgctcgctc ccgtccatga ggccgaatcg cattttcacg atcttttctt cgcgggggct
     6841 tagcgtcttg aggacttccg cggtttgttc acgaagattc aggttgatga ccgcttcgga
     6901 tggcgagatg ccctgacgat caacgatgaa gtcgccgagg tgcgactcct cctcttcgcc
     6961 aaccggcgtt tcgagcgaga tcggttcctg cgcgatccgc aataccttgc gaaccttgcc
     7021 tgccgacatc tccatcttcc gggccaactc ttcggtagtc ggctcccgcc gaagctcctg
     7081 ctggagttcc cgctgcgtcc gtaccagctt gttgatcgtt tcgatcatgt gcaccgggat
     7141 ccggatggtg cgcgcctggt cggcgatggc tcgagtgatg gcctgacgaa cccaccacgt
     7201 cgcgtacgtc gagaacttgt agccgcgccg gtagtcgaac ttgtccacgg ctttcatcag
     7261 gccgatattg ccttcctgga tcaggtcgag aaactgaagg ccacgattcg tgtaacgctt
     7321 tgcgatcgaa accaccaggc gcagattcgc ctcgatgagc tgcttctttg cggtctccgt
     7381 ctccagttcg ccccgctcga tgatctggag tgtgcgccgc aggtccaggg cagaggcgcc
     7441 gcagtcttct tcgagctgct ggagccgttg ggtggcatgg cgcagttcct tccggagttc
     7501 gcgcacaacc gccgcaccct cgtgcccgtg ctcctccaac cgccgctgaa tacgggcgat
     7561 cgtccgctcg acgggtctga aatcctccac tgcctgccgc accaggtcgg tgaatcggtt
     7621 cagcgccagc gggctaaact gaatctgccg gctaatgcgc gagaccgtca ccagactcct
     7681 tgccagcccg aagcgcagag tgcggtgctg cttcggtttc atcccccggg aaatcgcctg
     7741 gagtttttgg cgaaactgtt gagatttctt gtactgtttt ccgatttccg cgatctgcgc
     7801 atggagctca ctcttttgct tcgcgagcga gtcgtcgctg acgaccatgt ccggcagtat
     7861 caacagatcg cgcaattgca gcgtgtcacg atccagttct tccccaatgt gaagaacctc
     7921 gcggatcacc agggccgatc tcgagagagc gcggagcaca ctgcgctgac cacgctcaat
     7981 cctgcgagcc agttcaattt ctccctcgcg cgtgagaagg ggcaccgtac ccatctcgcg
     8041 tagatacatc cgcacggggt cgttggtctt ttcggagagc tctgtgctga tctcgagttc
     8101 ggcgagttcc tcgccttctt cgatcttttt ctcgaagtcg aggcgtggtt cttccaggat
     8161 ctcgacgcca gcgctgtcca ggtcggcgag aagctggtcc agttccgccc cgccaaccat
     8221 ctcgtccggc agcagctcat tgacttcgtc gtataggacg aagcccttct ctttcccagt
     8281 gtccatgagc cgcttgagct ggccgtattt ttcttcgatc accaagagaa ccccttcccg
     8341 ccagcaaccc tgaatccaac tctcaacatt ctacaatatg ctgccgctcg ccacccctag
     8401 acgcgttcgg cccggctcag ctcctccgcc agccgcaggg cctcttccat tctgccctca
     8461 cgctccgcct ccttgacccg ggatctcagc tccgcccgtt ttccctcccg ggcctcgacc
     8521 tccaatgccc ggacgcacgc cagcgcctgc tcccgtgtat agctcccctc acccaattca
     8581 tcggcaaaca ccaccgaggc gagtagagcc ttgtccccgt cgtccaggcg ggcttccagc
     8641 tctgagaagc cgaactccgg ccgactctcg tgcagcgtga acagtgttcg gaaaatccgg
     8701 cttgccggcg acttctctaa ttgcgggatt tgcgaaagcg ccgggacgat ctctccgcga
     8761 atatcgcggt tcgccaacag cgcattcagt aacaactgtt ccaccgcgcg tatgggttcg
     8821 cggtccacct gcattggcct gtcgctgcga ttggcggccg actttcggaa gtgctccagc
     8881 acaagaccct gctcgacgcc cagatacgtg gcgacgtcac tcgcaatcgc ggcccgctcc
     8941 agcttgtccg gcatccgctg gatcgccggt agcagaaatt gaagcgccgc tacacggccc
     9001 tctgcattcc gcatgtcgaa ccgcgcccgc gcccggtccg ccagccagta gaaatatcca
     9061 ggcgcttttt ccatcctgct acggtacgca tccgcgccgt gctctttgac gtattcgtcc
     9121 ggatccaggc ccccttcgag gtgcagcacc cggacgtgca tgccttcttc caggagcatc
     9181 tggatggagc gctcggcggc gcttgccccc gccgtatcgg ggtcgaagtt caccacgatt
     9241 cgctcggaat gccgcttcag cgcgcgcacc tgcgtggacg tcagcgccgt tccgcagctc
     9301 gccaccacct cgtgcacgcc cgcgccgaat actccgacga cgtccatgta gccttccacg
     9361 aggatagtgc gatcaagctt gcgaatcgtt tctttggctc ggtgaagatt gtacagaacg
     9421 tagcttttcc gatagatcgg ggtctcgggc gaattcaggt acttcggctc gtcgttggcc
     9481 gcgagcgccc ggccgccgaa tccgatgatc ttccccgact cgttgtgaat cgggaacatg
     9541 aggcggccgc ggaaacggtc aaagaaccct ccgccctcct gccgccgcag cagcaagcct
     9601 gacgcctcca tctgctccgc gctgaaaccc tcgcgctcaa agcgccgcac aaggtcctgc
     9661 ccggaacgct ccgaaagacc gatgccgaac tcctccgcga gtgagggcgg gacgccacgt
     9721 ctgccgaggt actgccgcgc ttccgctccc ggcgcgccgc cgagattcgt ctggaaagcg
     9781 cgtgccgcga tttcatgcat ctgatacagc gatgcccgca gctttgagtc ttcgtcggcg
     9841 tactccgccc gtttcggcat cgggatgccg ttgcggtcgg cgacgagctt cagcgcctca
     9901 tagaaactga ggcgctccac ttccatcacg aacttcagca cgtcgccgcc cacgccgcat
     9961 ccgaagcact tatagaactg gtgcgcctga tgaaccgaaa acgacggtgt cttctcggtg
    10021 tggaacgggc agagccccat gtagcgcggg cccgccccca cgcgtctcag gcgcacgtac
    10081 tccccgatga ccttgacgat gtcgaccgac gacttcagtt gctcaacgaa gtccatccgg
    10141 atgtcgcggc cccactcacc gcggttcgct tccgtctatt atcaccgaac acaaacgccg
    10201 ggaagagcag cgcccagccg gctgcgctca gcgtcgctcc acgccagagg atcgccggct
    10261 cgaagcgcat ctcaatccga tgccgtcctg cgtccacggg cattcctcga aaggcgacgt
    10321 tcgtcaacac aaccggtcgc tcgcggccgt ccacgaaagc tcgccagccc gggtagtacg
    10381 tttccgacgt caccaggaag gcaggcgccg cgctttcgac ttccagtgtg atactgcggg
    10441 gttcgtactt gatcagccga atggcgccgc ccggctgtcc ggcgctgccc ggaaactcga
    10501 caggttcctc caccaccgcg acttccgccg gccggaattc cgccgagcgc aattccgaga
    10561 gggcctcctc catgtttccg gcgtgctgaa tccggtcaac caggaagaac ctcggcaagg
    10621 cgtcgcggtt ctcgtagacc gtccgaccgg ggatctcagc caccggcaca aggcctctat
    10681 cccgcgtctc gccgaccggc gtccgcgaca gaaggtaccg cacgttcatc aaatccagca
    10741 cccgcgattc cggcttcaaa acctggtagt accgtcccca gcgctcgcct ccggtaaacg
    10801 taagccttac ttgcatgaac cgtatcagtg caaaaggatc gttcccgttg gccgtcggga
    10861 cctccgtcag agctgctccg tgcgcccagt tcatcgagtc gtcgatggtg tcgattcgcc
    10921 acggcggctt tgaccgtccc gtcaggtacc gcactctcgc aagtacttcc gtgctgccgt
    10981 cgaagctcga gtacgtcagg ccaggctctg tggcgagcga gccggtgttc aaattccggc
    11041 ccgagcccgc cgccgtcagt tccaccgccg ccaccgcgac gagcaatccg ccggcaagcg
    11101 gttttcggaa aatagcgaat cggtccgctc caagtcccgc cagcacggcc actcccagca
    11161 caaacgcggc catcgcgaat tccgggtaga cggaggactt cataatgttg ggcaacaccc
    11221 ggaacgcaac cttgcccacg ggcgtcgaag cacccagcat ccagagaatt gaaatgacca
    11281 gagcgatact gaagagtctc acctgccggt cccgccgaaa aatgaccgca gcgagcgcca
    11341 acaccagccc cgagatcccg caaaaaagat acaagaatgt cggctgccag gggtgcgcgt
    11401 atgtggagag atcgaagatc ccgtaatagt tcggcgctac cagcgacacc aggctcgcca
    11461 gtggaagacc tccgccgtcg ccgatccagt cactgcggta tttcgccacg ctgagccgtg
    11521 ttagttcagc ggtcggcaac acctgcaccg cggccagcag caggctccac actgccgcaa
    11581 gcgccatcgg cacaacggtc tttctccacc ccgctaggag gcccacgatc atcgtcgacg
    11641 ccgcaacgac gaacgtcacc gccgggaatc cggctagtat cgccatcgac aggctagccg
    11701 ccagcgctgc cgtccaccgc caggagaatc cccggctcag tgaaactgcg ctcagccaag
    11761 cgagcggcat ccatgccgct gcatccatcg cgcccaggtg ttgtgtttgg gatgcgaaat
    11821 agccgccgag ttgaaacacg gtgccgccga atagagcagc cgtcaccgaa gtgcccatgc
    11881 gtctcagcag caggtaagcc agcacaccgc ccaggaacac atgcagcacg agctgccatt
    11941 cgaggaagta gagcagattg ccgccgccgg ccagattgct cagccagacc gtcacgagcg
    12001 tcggtggata gaaaagctgc gtggtcaggt tcgcgtagat cggcataccg cagtaggtgt
    12061 atgggtccca cagcggcagc cctccgttgc ggaacgcccg cgccacgaac tcgatctgcg
    12121 gcagatggta gtaccgcaga tcccatggaa ttacgtaacg actgtctagg agaacctgcc
    12181 ggtaaaacac cagcacttcc accagcagca cagcggacgg gaccactcgg agtgcaagtc
    12241 ggcgcgtcac gggaacccct cagggtaaca ggaaacacac cgtggattga cgccgcgctc
    12301 ctaccgtttg cggaggatgg agaggtgatc gcggtgcttt gatcgcaggg cgataaagtc
    12361 ccggtggtcc aatttgagct cgctcgcgat ctggcgaatg acgttgtctt cctccatcga
    12421 aatcagcccc tcggcggcag caacagagaa caggcagtcg aggatcgtca gtttctgctc
    12481 gcgggtcgcg atgctcccga actcccgcgt gacgacgaag tcctccgtcg ccccgaacag
    12541 cttgttctgt gttttggcga tctgaacgac aaggatcgcc tgttcctcgg gaagcccacc
    12601 gtgttcgacg acgatgcgct ccatggcgcg ggtctccaac tcgctgatgt ggaggtccgc
    12661 ccgcgcagcc cgggcgagaa cgtaggcgaa cgcggcgatg taacgcgcct gatcgaagtc
    12721 catgcgatcg agggcgtcgg taatcttgcg gaccgtttct gtttcgggtg ttccggacgg
    12781 cggctccgtc tcgagcccca ggaatttcag gatcgacatt cggctctcgt aactacctta
    12841 gcgcgtcggg cagcgaagct gttcagagcg aactcacgcg agcgaccagg gctttcggta
    12901 ggggtacgtg atgaaacgat ccgcctcggg caggccggtc gcgcgctcgg tggcggcgtc
    12961 ccactcgatc ttgcggccgg tgcgcaaggc gacattgccg aggtgcgcaa cggttgagac
    13021 gaaatgtccg agttctacgt tttcgacggg gtcctggcgg gagcgaacgc agtccaggaa
    13081 attgcggacg tgggcgggac ggccgtcggc ggtgcgatcc tgcgggttga ccttccttgt
    13141 ccactcgccc agggtctttt tcttcggttc cgcgtggatc tcccagccgt ccgcgtcgag
    13201 gatcagggta ccttccgtgc cgctaaagga cagtccatgc gggcgccccc cgggaccgat
    13261 gccgccgagc agttgatgtt cccagacgag agtgtaggag gagaagtcgt agacggcgat
    13321 ctgggtgtcg ggggtctcgg agttgtcgtc gacaacgaat tttccgccgg aggaggtcac
    13381 ggagcggggc ggctccggtc ccatggccca gagggcgatg ttaatgaggt gcacgcccca
    13441 gtcggtcatg aggccgcctg cataatccca gaaccagcga aagttgaagt gaaagcgatt
    13501 cttgttgaag ggtcgttccg gggcggggcc gagccacatg ttgtagtcga cgctgggtgg
    13561 cggatcgctg tcggcgggct tgccgatgcc gccgacccag tcgagatacg cccaggctcg
    13621 tacctggcgg atgcggccga gcttgccgga acgaacgaag tcgaccgctt cctggtagtg
    13681 ggggccgctg cgccactgcg tgcccatctg tacgatgcgc ttgtgattcc gggtggcggt
    13741 caccatcgcc cggccctcgc cgatggaggt cgccagcggt ttctcgacgt aaacgtcctt
    13801 gccggcctgg caagcgtaga tggcgggcag cgcgtgccag tggtcgggtg tgcagatgag
    13861 gcagacgtcg atgtccttgc ggtcgattac gcggcggaag tccttgacag tgtcgggcgt
    13921 tgtctggcga tgcttttcga ccaggctggc gcgtttggcg atacgttcgt cgtcgatatc
    13981 gcagacgatg gggcagtcta cctcgggatt caggaagaat gttggcaagt cggcgcccga
    14041 gatgccgcca cagccgatga gaccgacgcg gatcctgtcg ctgggagcgg cggtggcgaa
    14101 ggagcgaatg aatgccgggg cggcgccaag cgcggtggcg gagcggccaa ggaatgaccg
    14161 gcgggtagtg tcggacatgg gtggagcctc ctgcgtttca gggcagagtg tatcacgacc
    14221 gccacaggct gaagccggct gaagcttgtg ccaccgaagg cagtaagcag tcgattctca
    14281 gaggcggtca ggctctacag ggcgacggag gaaagtgaaa cggctcgtca cccggcagcc
    14341 gcccgcttga gcgcggcaat atcgaacttc ttcatcttga gaaaggcctg ggttaagcgc
    14401 gcggtccgct cccggtcctc gcctccgagc aattcgcgca taacggcggg agtgatctgc
    14461 catgagacgc catacctgtc cttcagccag ccgcactgtt cggctctcgg gtccgccgag
    14521 agtttccccc agaagtaatc gatctcttct tggctatcgc agggcacgat gaacgatatc
    14581 gcctcgttga atgcgaactg gtgcgcccgc gcggaatcca tcgcgccgaa ctcctggccg
    14641 agaagcgaga ggtgcgcgta ccgcacggtg ccttccgtgt ccggatcctc gcccttgcca
    14701 taacgagcga aaaccgtcgc tttcgtttct cccacctccg cgccattagg cgcattcctg
    14761 aacaccgagg cataaaagct gatcgcttct tcggccttgc cgcacacctt gcccacgaac
    14821 atgagcacgg gcgtgaacct ctgcttgatc tccattccgc cagccaatat cacctgccat
    14881 gaaagcccgt accgatcttc gagccacccg taccgctcgc taaagggata gctgtcgaga
    14941 ggcatgagga ccttcccgcc aggagagagc ttctcccata tcgcatctac ttcctctttc
    15001 gttccgcatt tggcatggaa ggacacggac ggattgaact tgaacaaggg cccagcgccg
    15061 atcgccataa agggctgtcc cgcgagctcg aacgacacga ggtcgcaatc gcccgaaggc
    15121 gtgcctgaga gcctggtcac gttggtgatc cgggacccgg gaaacaagga cacatacaat
    15181 tcggccgcct cccgcgcctc cttatcgaac cacagatgcg gagtgatctt ttgcacaatg
    15241 ttttagccgc cccaaacaga cgggcaacgc gccccctccc tcaccggaat tataccggca
    15301 cggcgcactc taattcgcgg cgatatgcga ccggcttgac cgattcctcc cggttaaccg
    15361 cctgttacag ggagccggcg cagggaacca taggcgaggg tcccgcaggt gcggctcgac
    15421 atgttcggca tcagaaatct ctattgaggg gacacgttgc gatcagcgag aggctgtgag
    15481 gcgctggcgt cccattttcg gacagctggc acccagaagc gatcactggc gggcctcagt
    15541 gttgagtaag atcaaggact tagagtcgga agctaaattg cccccaagga caggaaccac
    15601 atgcgagacg tccttaaaga cctccagtac gcggtgcgtg tcctccgccg ggaccccggc
    15661 attgacagcg gtcgcagtgc ttaccctggc gctcggtatt gccgccacca cagccgtctt
    15721 tggttggatc gacgggatgg tcctgcaccc tttgcgcggc gccgcggacg acggacaact
    15781 cgccgtcctc gaagccgtcc gtgccagtgg catcgaggat cctaacgtct cgtatgccga
    15841 ctgccgggat ttccaggaca gcctccgaag tatctcagga ttggtgctga accaacaggc
    15901 ccccgcctca gtcggcgacg gagagaacgc ctattccgcg tggttcgaac tggtttccgg
    15961 aaactacttc gacgtcttgg gcgtaaagcc agtcctgggc cgcacgttcg ctcgcgagga
    16021 gtacggcgac agggccaaag cgttcacggc ggtcatcagc taccggatgt ggcggcacta
    16081 ttttcagaac gacccgtcca ttctcggccg caccgtacac attaaccggc accgggtcac
    16141 catcgtcggc gtggcgcctc cggagtttca cggcgatatt ccggggatcg cgtttgacgg
    16201 ctgggttcca gcgccactga ccggcgaacg cgaccgcgat gcgcggcatt tcagggccat
    16261 cgtcaggctc aaacccggtc tcagcgtctc cgaggcaaat gccgaagtag cgacggttgc
    16321 agcgcgcctt gcccgagcct ttccgacgac caacgagggg atcggcgccc ggatcgtgcc
    16381 gatctggaag gcgcagtcgg gattgtcggg cattttggca tcgccgctgg ccatcctcgg
    16441 tgcggcctgc ggactcgtgc tactcattgc gtgcgcgaat gtcgccaatc ttctgctggc
    16501 tcgttccgca gcccggcaga cggagttcgg catccgcgcc gccctgggcg cgggtccggg
    16561 ccgattgagc cgacagttgg tttgcgaaag ccttcttctg gcggctctgg cgacgcttgt
    16621 ggggctgcct ctcgcgctgt ggtctcagga catcctcgtg tatctcgtgc cgcctacggg
    16681 attgccgctg tacttcaacg tccacccgag cgctcggcag ttcctttttg ccgctctggt
    16741 ctgcctggct tccgccctga tctccggtct tccgcccgcg tttcagtcgg tgcggcgcag
    16801 cctagtggat gcactgaagt aaggaggaag aggtgacaca cagggcgggt actcgcggcg
    16861 catctccggg ttactggtgg tatccgaagt ggggctggcg ctggcggctc tcgtgacgct
    16921 cggactgttt ctccggagcc tttacggatt gcagaacact ccggccggtt tcgatcaccg
    16981 caacgtaacg gtttgccgtc tctttctggc gacaaacaac tacactccca acgaggagca
    17041 gcagttctcg cggaggctcc gcgagcggct gcgtgccctg ccaggggtgt caggcgccgc
    17101 tttattccga ctcgatcccg ctcggcttcg ggctcgggaa atggaccgag gttatggtcg
    17161 aagggtatgc ggcgaggccc ggcgagaacc tggacgtgca tcacgcctcc gtgtcgtccg
    17221 gctacttcga cctgctgagg gtgccgcttc tcgccggacg cgatttcagg cctgaggaca
    17281 acgaaaaggc gccccgcgtt atgatcgtca acgaatcctt cgcgcggcgc tttttcgacg
    17341 gacgcgaccc ggtggggcac cgggtacaca tttatggcat accgttcacg attgtgggaa
    17401 tggtgaagga cagcaagtat tgcagcctgt ctgaagcgcc gcagccatac ttctatatgt
    17461 ccttcgacca ggtacacaac ggcagtggcg aaggcggcgt ggccctttat gcccggaccg
    17521 acggcgatgc gcgcggtttc gtccccgtgc tccgccgcga gatgtcggcc atcgatccga
    17581 actcggccgg cctgacggcc atgcccctgt cggattacat ctcggctgcc tggttcgcgc
    17641 cgcggctcgc ctcgttgctc ctcggagtgc tcggcgtcat ttccatgctg ctcgccggtg
    17701 taggtcttta cggagttatg gcttactcgg tgagccagcg gacccgggaa atcggtatcc
    17761 gcatggcgct gggggctgac cccggaggcg tgctccggac ggtcatgaga cgaggcttgt
    17821 tgctggcctt gtcggggatc gccgccggac tcgccatctc cctggcagcg accccgcaga
    17881 tcgagcctct gctgtaccgt gtcagtccag cggaccctgt aagcatagcg ggagcagcgc
    17941 tgttcctcat tgcggttgcg gtggtcgcaa gcatcatccc agcgctgcgc gccaccaggg
    18001 ttgacccgat ggtcgccctc cgccaggagt gactaaccga caagctgctt gcgcgccgcg
    18061 taaagcacca tttcggcaac attgcggagc ttcagtttgt gcatgatgtg ggtgcggtgg
    18121 gtctccacgg tgtat
//
LOCUS       DATIFQ010000467         2895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2264243, whole genome shotgun sequence.
ACCESSION   DATIFQ010000467 DATIFQ010000000
VERSION     DATIFQ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264243"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VLE22_23575"
     CDS             complement(<1..237)
                     /locus_tag="VLE22_23575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17450.1"
                     /translation="MTPTNNQTNDSGIHEGASLSRRDFLVSGGSTLALLAMMDSSLFA
                     RMAGAADDGEVIPFIDRPPAAPEAAVRAYGELNRL"
     gene            complement(277..1098)
                     /locus_tag="VLE22_23580"
     CDS             complement(277..1098)
                     /locus_tag="VLE22_23580"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB17451.1"
                     /translation="MSASTVVSETVGLKRRLQQIWMAGDYDLFSRYMEGSAREFYERL
                     HVAPGSKLLDVGCGSGQLALMAAREGAEVTGVDIAANWVERARVRARAEGLLARFEEA
                     DAESLPFEDGAFDVVVSLVGAMFAPRPDLVAHELLRVCAPGATVAMANWTPQGFVGQM
                     FKIVAKFIAPSGMPSPTLWGDEPTVRERLGKGLSELNLVRHHYTFSYPFPPSEVVEFF
                     RLYYGPTHEAFAALDEHGQDRLRHELETLWTAHNRGGSGSTIVFAEYLEVIGIRA"
     gene            1294..2310
                     /locus_tag="VLE22_23585"
     CDS             1294..2310
                     /locus_tag="VLE22_23585"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HSB17452.1"
                     /translation="MDVLSEVLRAVKLDGAVFFNGEFSAPWCMREPDACTMASYLSAP
                     SKHVIIFHLIAEGRGHARVEQDGGVISLAAGDIVMFPHGDAHLMGNGPPVTPVDSAQQ
                     LQQVLAEGRVLSQFGGGGELTKVICGYMTCERHLSQVFLAGLPPIIKINIRDHASGQW
                     LEDTLRYSVAHADAAGPGGAAVVAKLSEALFVETLRRYIAQLPPAQTGWLAGVRDPEV
                     GKALALLHRQPAQPWTIATLANEVGVSRSVLAERFRHYLSDTPIGYLTRWRLQLAAQL
                     LTSTSKSVAEVAGQVGYESEPSFNRAFKREFDFPPARFRSQARTPRHITTGSIRGTSG
                     ASKR"
     gene            2623..>2895
                     /locus_tag="VLE22_23590"
     CDS             2623..>2895
                     /locus_tag="VLE22_23590"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSB17453.1"
                     /translation="MYIRTLKAVIAVLGGLAAIAVTFEPVLAEPLIVGAPPSLRPAFT
                     DILPMFEREYDVAVHVVYTPSKTLMREIENGAPVDVFLAAGFDEVEH"
BASE COUNT          561 a          915 c          816 g          603 t
ORIGIN      
        1 cagccggttc agttccccgt acgccctcac cgccgcctcc ggagcggctg gtggccggtc
       61 aataaacggg atgacttccc cgtcatcggc cgcaccggcc atccgtgcga agagtgaaga
      121 atccatcatg gccagcaggg caagtgtcga gcctccactg acgagaaagt cccgtcggga
      181 gaggctagcg ccttcgtgta tgccgctatc gttcgtctgg ttattcgttg gtgtcatggt
      241 gtccctcctg tggttccgct tttttctctc tctcacttac gctctgatgc cgatgacttc
      301 cagatactct gcaaacacga tcgtactgcc agacccgcct cggttgtgcg ccgtccagag
      361 tgtttcaagc tcatggcgga gccggtcttg gccatgttca tcgagcgcgg cgaaggcttc
      421 atgggtcggt ccatagtaaa ggcgaaagaa ctccaccacc tctgagggcg gaaagggata
      481 actgaacgtg tagtggtgcc tgaccaggtt gagttcggac agtcccttgc ctagccgctc
      541 gcggaccgtc ggttcgtcgc cccatagcgt gggggacggc atcccggaag gcgcaatgaa
      601 tttggcgacg atcttgaaca tctgccctac aaatccctgt ggcgtccagt tcgccatggc
      661 cacggttgcc ccaggtgcac agacgcgcaa gagttcgtgc gccaccagat ccggacgcgg
      721 ggcaaacatg gcgcctacta aactcacgac cacatcgaac gcgccgtcct caaagggcag
      781 cgattcagcg tcggcctcct cgaaacgtgc cagcagcccc tccgctcgcg cccgaacccg
      841 cgcccgttcc acccaattgg ccgcaatatc gactccggtc acctccgccc cctccctcgc
      901 cgccatgagc gccagttgac cggagccgca gccgacatcc agcagcttgg atccgggagc
      961 gacatggagc cgctcataaa attctcgcgc gctcccctcc atgtaccgtg agaacagatc
     1021 gtagtcgccg gccatccaga tctgctggag ccgacgcttc aacccaaccg tttcggaaac
     1081 taccgtgctg gccgacataa agccctcctg tttttgcatg gtgatatcgc cgacacgccc
     1141 atcgtgtggc gctacccact gtatcgccag gagagacggc tgtcttatca gagcgtctgg
     1201 ctctcttctc aagagaatgc taagctgcgc attcttgtat ccgagcgtcc ggcctgttta
     1261 gcagacgctc cttccctgct gcatggaacc ccgatggatg tgctatctga agtgctcagg
     1321 gccgtcaaac tcgacggcgc agtgtttttc aacggcgaat tctctgcacc ctggtgcatg
     1381 cgggaacccg acgcctgcac catggcatcg tatctgtccg ccccttccaa acatgtgatt
     1441 atttttcatc tgatcgccga gggccgtggc catgcccgcg tggagcaaga cggcggcgtc
     1501 atctcccttg cggccggtga catcgtcatg tttccgcacg gcgacgcgca cctcatgggt
     1561 aacggtcccc ccgtaacgcc cgtggacagt gctcagcaac ttcagcaggt gctcgctgaa
     1621 ggaagggtgt tgtctcaatt cgggggaggc ggggaactca ccaaagtgat ctgcggctat
     1681 atgacctgcg agcgacacct gagtcaggta tttcttgccg ggttgccgcc gatcatcaag
     1741 atcaatatcc gcgatcacgc atcagggcaa tggctggagg acacactccg ctattcggtc
     1801 gcccacgccg acgccgcagg cccgggcggc gcagcggtcg tcgccaaatt gtccgaagcg
     1861 ttattcgtgg aaacgttgcg gcggtacatt gcccagttac cgccggcgca gaccggctgg
     1921 ctcgccggtg tgcgtgaccc ggaggtggga aaagccctgg ccctacttca tcgacaaccg
     1981 gcccaacctt ggacgattgc cacgctggcc aacgaagtcg gggtgtcccg ctccgtgctt
     2041 gccgagcggt tccggcatta tctttcggat acacccatcg gttacctcac gcgctggagg
     2101 ctgcagctcg ccgcacaact tctgacgtcg acgtccaaga gtgtcgctga agtggccgga
     2161 caggttggtt atgagtccga gccctccttc aatcgggcct tcaagcgcga gttcgacttc
     2221 ccgcccgccc gattccgcag tcaagcgaga acgcctcgcc acattacgac cggttctata
     2281 cggggcacca gtggagccag caaacgataa gcgtaccggt tctggcccca ttgcccgcgc
     2341 gcagcggcct ttcgaggcct ccccgctcga ccctctccga ctactcaata ttcagaactc
     2401 aacattacga acatcactct ttactcctca cccctgccac cgtcgtcgaa tgtgaagtta
     2461 acttcagtcc ggcagggatt gcgcctcaga cgtaagcaag gtacaagggc attcgcggac
     2521 gtactgatcc ggtagtacga atgcctccgg ctggtcgata gttaccggat ttggtaagct
     2581 cggcaacaat agggccgctg attgatacga gagaggttta gagtgtacat tcgcacgctc
     2641 aaagccgtga tcgctgttct gggtggactg gcagccattg cagtgacctt cgagccggtg
     2701 ctggctgagc ctctgatagt cggcgcccct cccagcttaa gacctgcttt taccgacatt
     2761 ctgccgatgt tcgagcgaga gtatgatgtg gcagttcatg tggtatacac gccgtcaaag
     2821 acgctcatgc gggaaatcga aaacggagcc ccggtcgatg tgtttctggc agcggggttt
     2881 gacgaagtgg agcac
//
LOCUS       DATIFQ010000468         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1308890, whole genome shotgun sequence.
ACCESSION   DATIFQ010000468 DATIFQ010000000
VERSION     DATIFQ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308890"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..>2047
                     /locus_tag="VLE22_23595"
     CDS             282..>2047
                     /locus_tag="VLE22_23595"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HSB17454.1"
                     /translation="MNRPVCRVMVAVAGMAVLGMTATRAGAQTHPATSTLNDVMQAPF
                     ASDMLAAPTGAAVAWVFNAKGCSNIWVADPSHGAKARQITPYTEDDGFDIGELAWSPD
                     AKSIAFTRGQSLEDETPANVNTSPEGAMPREVWVVATDGGAPRKVGAGDSPTFSPDGS
                     RLLFVDKKRILTVAASGDGDAQPLLIDQGTVDSLTWAPDGKRLAFVSHRGGHSLIGVY
                     DFVDQTIVWLSPSLDYDSSPAFSPDGARIAFVHVPAQKTPPAFFSQRSGRPWSIWTAD
                     VKTGQGRRVWIADAGPGSVFHPTRSATNDVPDVPSATNLFWTNHDDLVFPWEKSGWLH
                     LHAVPVRGGGARAFTAGKFEVTHVVFSQDRKRLVYSSSQDDTDRLHIWTVDAEHGSAV
                     RTAQSHAIEDYPQIGADGALFALQSDANQAPHPVALSPGGQWRRLAPEAIPSSFPSSQ
                     LVMPQAVTFTAKDGQETHAQLFLPHETTSKPHPAILFFHGGPQRQMLLGFDPKRAYDW
                     IYAFNQYLVAEGYIVLSVNYRGGTGYGLDYREADSFGPGGSSELNDLLGAITYLRGRQ
                     DVDSHRLGLWGPSYGGLMTALGL"
BASE COUNT          407 a          645 c          627 g          368 t
ORIGIN      
        1 cgagtaactc agcacccaat tcagcctcca gcctttgcag ttgtcgggtt atagccgatg
       61 gtgtccggaa tagacgatca gctgcgccgg caatggaccc ggcctgggca aactctacga
      121 gggcgcgtag atcatcgagc aagggagcct cctgaagtct cctaaagcct cttccagagt
      181 agcagaagcg tgcgtaaacg gcaacattat cgtgatgata atgaacttgt ggaattatgg
      241 gttctccgtt acaaccggtg agtagaggca ggggaggtag cttgaacaga ccagtgtgta
      301 gagtgatggt ggccgtcgcc ggcatggcgg tgctcgggat gaccgcaacg cgggccggcg
      361 cacaaaccca tccagcgact tctacactca acgacgtgat gcaggcgccg tttgcgtcgg
      421 acatgctggc cgcgcccacg ggcgcagccg tggcgtgggt attcaacgcg aagggctgca
      481 gtaacatctg ggtagcagac ccatcccatg gcgcgaaagc gcgccagatc acaccgtaca
      541 cagaggatga cggtttcgat atcggcgaac tcgcctggtc gccagatgcc aagtcgatcg
      601 ccttcacacg gggccagagt ctcgaggacg agactccggc caacgtcaat acctccccgg
      661 aaggcgcaat gcccagggaa gtatgggtgg ttgccacgga cggcggcgcg ccgcgtaagg
      721 tcggcgcggg agactcccct accttctcac ccgatggcag ccggttgctg tttgtcgaca
      781 agaagagaat tctgaccgtg gccgcgagcg gcgatggcga cgctcagcca ttgctcatcg
      841 atcagggaac agtggactcg ctgacctggg cgccggacgg caagcgcctg gcgttcgtga
      901 gccatcgagg cggccactcg ctgatcggtg tctacgattt cgttgaccaa accatcgtct
      961 ggttgagtcc gagtctcgac tacgacagct cgcccgcgtt ctcaccggac ggcgcacgga
     1021 tcgccttcgt tcatgtgccg gcccagaaaa ctccccccgc attcttctct caacgcagcg
     1081 gacgaccgtg gtcgatctgg acggcggacg tgaaaaccgg ccaggggcga cgtgtctgga
     1141 ttgccgacgc gggacccgga agtgtattcc atccgacacg ctcggcgacg aatgacgttc
     1201 ccgacgttcc ctcggcgacg aacctgttct ggaccaacca tgatgatctc gtgttcccct
     1261 gggagaaaag cggctggctg catctacatg ccgtccccgt gcggggcggc ggggcccgcg
     1321 cattcacggc gggaaagttc gaggtcacac acgtcgtctt cagccaggac cgcaagcgcc
     1381 tggtctattc gtccagtcag gatgacaccg acaggttgca tatctggacg gtcgacgccg
     1441 agcacggttc ggcggtgcgc accgcccaga gccacgccat cgaagactat ccgcagatcg
     1501 gcgccgacgg cgctcttttc gcattgcaga gcgacgccaa ccaggcgccg catccggtcg
     1561 ctttgagtcc gggggggcaa tggcggcggc tcgctccgga agccatcccg tcctccttcc
     1621 cgagttccca gctcgtcatg ccccaggccg tgacctttac ggcgaaggat ggccaggaaa
     1681 cgcacgcgca actgttccta ccacacgaaa ccacctccaa gccgcacccg gcgattctgt
     1741 tcttccacgg cggccctcaa agacaaatgc tgctggggtt cgatcccaag cgcgcgtatg
     1801 actggatata cgcatttaac cagtacctcg tcgccgaggg ctacatcgtc ctgtcggtca
     1861 actatcgcgg cggtaccggc tatggccttg actaccgaga ggccgacagc ttcggccccg
     1921 gcggcagcag cgagctcaat gacctgctcg gcgccatcac ctatctgcga ggccgacagg
     1981 acgtcgactc ccatagacta ggcctctggg gaccaagcta tggggggctc atgacggccc
     2041 ttggtct
//
LOCUS       DATIFQ010000469         5288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1309038, whole genome shotgun sequence.
ACCESSION   DATIFQ010000469 DATIFQ010000000
VERSION     DATIFQ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309038"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VLE22_23600"
     CDS             <1..730
                     /locus_tag="VLE22_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17455.1"
                     /translation="GPDFPLVDYHVHIEREMTIERALKIAVERGVKIGVAEHGGCAEE
                     MRDDAGLRRYIERLSPYPVYKGMQAEGLDWARCFSKEAVAELDFVLSDALTFPEKDGR
                     RVRLWTKEAKIEDKQDFMERYVDFNVRVIATEPIDIFANPTFLPEGIVGEYDTLWTAG
                     RMKRVIDAAVEHEVAIEINALYRIPSLAFLRTAKDAGVKFSFGSNSHGEGIGKLDYCL
                     EMAKALGLSSKHMFSPDSRPGRSL"
     gene            727..1449
                     /locus_tag="VLE22_23605"
     CDS             727..1449
                     /locus_tag="VLE22_23605"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HSB17456.1"
                     /translation="MNRRDLVAGLALASMGTEAQQQDTESLYIPQQHRVEDRDLLHDF
                     MDEFPFADLVTSTPAIRITHIPVVLDRTAGKYGTLYGHVSRQNPQSRMLDGQHPSLVV
                     FHGPHGYISPTWYEKTEVVPTWNFAVVHVSGKPRPVTEKKALNDLLRKLIDRFEKYET
                     SAYDFSKLSDSYKYGLMAGIVGFEMEIELIEGKFKLGQERSEADRASVLRHLRSASPP
                     RSLHDLTASFYERTKAAGKGMR"
     gene            complement(1626..>5288)
                     /locus_tag="VLE22_23610"
     CDS             complement(1626..>5288)
                     /locus_tag="VLE22_23610"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB17457.1"
                     /translation="ALLAPTAWPQASTATVSGTVRDQSGAVVPTASVTLTNSATNIVS
                     KTTTTTVGIYFFLGIVPGPYEILVEAPGMQKFEGKLTVQVQQSAVVDVVLKVGQTATE
                     VTVQDVTPLITADSPTLSHVLERERIEQLPINGRFITALLQTVAGLELDGSDLDSTTA
                     RAFGMKRGSTEFVLDGAATTDRLSGAVFRRPAGLDTIQEFRVESNVSSAKLARPTTVI
                     MSTKSGTNELHGSAFETARNNGVGKARTRTDFYDKPPHLVRNEFGVSAGGPVYIPKVY
                     NGRNRTFWFFAYEAMRDLEARTMGFAVPTEAMANGDFRGLVDAEGVPFEVYDPWTTNS
                     ETWERQRISYKGQLNVIDPARESPLAKYLFSITPKPTNSANPLVAPNFYGPQPNRRRD
                     WTVTTRIDHQFSEKDQFYGRYTQGKYDLFTQFYGFVALNNVGSTVSRTAPNKAGAGSW
                     VHTFSPTFFNEVLVSGSSEHWTSSTGDPNRKYADELGLPNPNNVAGWPAMYDLGSMYD
                     WYFETENTQTTPFAFWIADDNATKIAGKHELQFGFHFRWDKLNTLAEQQYTQGEIIGN
                     SIFTSLYDTGSDRTNPLPLDYTGDATASTYLGLVNYYAQLGHPWFQLRAKEYALYFQD
                     NWKVTPRLTLNLGLRWDRWPPNRDISNLLTTFDPANKAVVLASSLDHMYEVGATLPSV
                     VQRLQSLGVKFESYKDAGLPQTLYTTPNTDFGPRAGFAYRLTSGNRSTVLRGGYRISH
                     FTIPLRAWTAQQRSNTPYFARLTSNINGSALSPDRINNYYMRSVPTIVAGVNSRDAID
                     LENATSITRGSASNYYFAKEQPDTRVHDWNLTLEREVMANTVVRASYVGNHSSNLQVK
                     NSYNDSTPDYIWYATTGLPLPTGEYSGVARRPFDQTVYGTLQEYQKSAFGNFNGMTLE
                     AERRYSGGYAFQLIYTLGNSLSAGGDTYRSVVNSPNSYLPGAVPADFSDRMRFLNYKR
                     DADVANNDIPKHRVRWNWLVDLPFGRGKPLGRNVGTFLDKIIGGWQLAGIGNLRSTYF
                     ALPTNIFPNGTPIEVYGYKYPIQDCRSGECLPGYLWWNGYIPANRINSTDPETGKPNG
                     VMGVPANYTPAAQPLIPWPAVPDRSDPLYRYYGTNTVFVPLKNGTSQRVTYNDNLNPW
                     RNQFVPGVRQWGLDASLFKAIRFKERYEVRFNADFFNVLNHPGNPNSIGGDGVLTTNN
                     SGQRARELQLTLRLSW"
BASE COUNT         1062 a         1508 c         1590 g         1128 t
ORIGIN      
        1 cggacccgac tttccgctgg tagactacca cgtccatatc gagcgcgaga tgaccatcga
       61 aagggcgctc aagatcgcag tggagcgagg agtcaaaatc ggcgtcgccg agcacggtgg
      121 ctgtgcagaa gagatgcggg atgatgcagg actgcgccgc tacatcgaac ggctgtcgcc
      181 ctatccggta tataagggaa tgcaggcgga ggggctggac tgggcgcgct gcttttcgaa
      241 agaggccgtt gcagagcttg attttgtgtt gtcggatgcg ctgacgttcc cagagaaaga
      301 cggccggcgg gtgaggctct ggaccaaaga ggcgaagatc gaggacaagc aggacttcat
      361 ggagcgatac gtggacttca acgtccgcgt gatcgctacg gagccaatcg atatcttcgc
      421 caacccgacg ttcctgcccg aagggatcgt gggggaatac gacacgctct ggacggcggg
      481 acggatgaag cgggtgatcg atgcggccgt ggagcacgag gtcgcgatcg agatcaacgc
      541 gctctaccgg attccgagcc ttgcgttcct tcggacggcg aaagacgctg gggtgaagtt
      601 ctcgttcggc tcgaattctc acggagaagg cattggcaag ctcgactact gcctcgagat
      661 ggcgaaagcg cttggactgt cgagcaaaca catgttctcg cctgattccc ggcctgggag
      721 gtcattgtga accgacgcga tctggttgcc ggcttggccc tcgccagtat ggggactgag
      781 gcccaacaac aggacacaga gtccctttac atacctcagc aacaccgagt tgaagatcgg
      841 gacctcctcc atgatttcat ggatgagttt cccttcgccg atcttgttac ctccacgcct
      901 gccattcgca tcacgcatat cccggtagtg ctcgatcgga ccgccggcaa atacgggact
      961 ctatacggtc acgtttcccg tcagaatcca cagagccgga tgcttgacgg ccagcatccc
     1021 agcctggtcg tcttccatgg acctcatggc tacatctcac ccacgtggta cgagaagacc
     1081 gaggttgttc cgacctggaa tttcgccgtt gtgcatgtta gcggcaaacc caggccggtc
     1141 acggagaaga aggcactgaa cgatctgctt cgaaagctca tcgacaggtt tgagaagtac
     1201 gagacctccg catacgactt cagtaagctt tcggacagtt acaagtacgg cctaatggcc
     1261 ggcattgtcg gcttcgagat ggaaatcgaa ctgattgaag gcaaattcaa actcggccag
     1321 gaacgcagcg aggccgacag ggctagcgtc ttacgtcacc tccgttcggc gagtccgccg
     1381 cgttcccttc acgacctcac cgccagtttc tatgagagga caaaggctgc ggggaagggc
     1441 atgcgctgag gggcgctacg gcacgaaacg ggatctccgc caaggcatca ctggtaccgg
     1501 cacctgattt gcacagcccc acaccgctgg acgttgaaga agcaaacaaa cgcccaagcc
     1561 gaggatcctt ttgagatcct cagccctgct cgtgttcgaa tgaagaagcc ttttctgctt
     1621 atgggttacc agctcaaccg gagcgtaagc tgcaattcgc gggcgcgctg gccgctgttg
     1681 ttcgtagtga ggacgccatc cccgccaata ctgttaggat tgccggggtg gtttagcacg
     1741 ttgaagaagt ccgcgttgaa gcggacctcg taacgctcct tgaacctgat cgccttgaac
     1801 agcgatgcgt ccagacccca ctgccgtacg cccggcacaa attgattccg ccagggattc
     1861 aggttgtcgt tgtaagttac gcgctgcgac gtgccgttct tcagcggtac aaaaactgta
     1921 ttggtcccgt aatatctgta cagcgggtcg cttctgtccg gaacggccgg ccacggaatc
     1981 aggggctgcg ccgcaggcgt gtaattcgct ggaacgccca tgacaccatt cggcttcccg
     2041 gtctccggat cggtgctatt gatgcgattt gctggtatat acccattcca ccacaagtat
     2101 ccggggaggc actcaccact gcggcagtcc tggatcgggt acttgtagcc gtaaacctcg
     2161 atcggtgtgc cattcgggaa gatgttggtc ggcaaggcga agtaagtgct gcgcaaattg
     2221 cctatccccg ccaactgcca gccaccgata atcttgtcga gaaacgtgcc gacgtttcgg
     2281 ccgagaggtt tgcccctgcc gaagggcagg tccacgagcc agttccagcg cactcggtgc
     2341 tttgggatat cgttgttggc aacgtcggca tcgcgcttgt agttcaggaa gcgcatacga
     2401 tcggaaaagt ccgccgggac tgcccctgga aggtacgagt ttggtgagtt gaccacggag
     2461 cgatacgtat cgccccccgc cgagagcgag ttgcccaggg tatagatcaa ttggaacgca
     2521 tacccaccgc tgtagcggcg ttcggcctcc aaggtcatgc cgttgaaatt accgaaggcg
     2581 gacttctgat actcttggag ggtgccgtag acagtctggt cgaagggccg gcgcgccacg
     2641 ccggagtact ctcccgtcgg tagcggcagc cccgtcgtag cataccagat gtaatccggg
     2701 gtcgagtcgt tgtaactatt cttcacctgc aggttactgc tgtgatttcc cacgtaagag
     2761 gcgcgcacga ccgtattggc catcacttcc ctttccaggg tgagattcca gtcatggacg
     2821 cgcgtgtcgg gttgttcttt cgcaaaataa tagttgctgg ccgatccgcg ggtgatagag
     2881 gtcgcgttct ccaggtctat cgcatcccgg ctgttaacac cggcgacgat cgtgggaacc
     2941 gacctcatgt agtagttgtt gatcctatcc ggcgagagcg ccgaaccgtt aatgttgctc
     3001 gtgagacggg cgaaataggg agtgttggag cgctgctgcg cagtccaagc gcgtagggga
     3061 atcgtgaagt gggaaatacg gtaaccgccg cggaggacgg tgctcctgtt gccgctcgtc
     3121 aggcggtagg cgaagcccgc cctgggaccg aagtcggtgt tcggtgtggt gtaaagggtt
     3181 tgtggcaggc cagcgtcctt ataactctcg aacttgactc ccagcgattg tagtctctga
     3241 accaccgagg ggagggttgc cccgacctcg tacatgtggt ccaggctgct ggcgagcacg
     3301 accgccttgt tcgccgggtc aaatgtagtc agcagattgc taatgtcccg gttaggcggc
     3361 caccggtccc agcgcagccc caggttcagc gtcagccgcg gtgtcacctt ccagttgtcc
     3421 tgaaagtaca gggcatattc cttggcgcgc aactgaaacc aggggtggcc gagctgagca
     3481 tagtagttaa ccaatcccag gtaagtgctc gccgtggcgt caccggtgta gtccaatgga
     3541 agcgggttcg tgcggtccga acccgtgtcg tacagggatg tgaaaatgct gttcccgatg
     3601 atttcgccct gcgtgtattg ctgctccgcc agggtattca gcttgtccca ccggaaatgg
     3661 aatccgaact gcaattcatg ctttccagcg atcttggtcg cgttatcgtc ggctatccag
     3721 aaagcaaacg gggtcgtttg agtattctcg gtttcgaagt accagtcgta catgctgccc
     3781 aggtcgtaca tagcgggcca gccggcaacg ttgttcggat tcggcagccc gagctcgtcg
     3841 gcatactttc tattcggatc gcccgtgctg gaggtccaat gctcgctcga accactgacc
     3901 agcacttcgt taaaaaatgt cggcgagaaa gtatgcaccc aggagccggc gcccgccttg
     3961 ttcggagcgg tccggctcac cgtgctcccc acgttgttca aggcaacaaa accgtagaat
     4021 tgcgtgaaca ggtcgtactt gccctgcgtg tatcggccgt agaactggtc cttctccgag
     4081 aattggtggt cgatacgggt ggttaccgtc caatcgcggc ggcggttggg ttgcggcccg
     4141 tagaagttgg gcgccactaa tgggttggcg gagttcgtgg gctttggcgt aatgctgaac
     4201 agatacttgg ccagcgggct ctcccgggca ggatcgatca cgttcaactg ccccttgtaa
     4261 gagatccgct gccgctccca cgtctcggag ttcgtcgtcc aggggtcgta gacttcaaat
     4321 ggaacacctt cggcgtcaac caggccgcga aagtcgccat ttgccatcgc ttctgtcggc
     4381 acggcgaagc ccatcgtcct agcttccaag tcgcgcatgg cctcgtaagc aaagaaccag
     4441 aaagtacgat ttctgccgtt gtaaaccttc gggatgtaca ctggcccgcc cgcggagacg
     4501 ccaaactcgt tgcggactaa gtgcggcggt ttgtcataga agtcggtgcg cgtgcgggct
     4561 ttgccgaccc cgttgttgcg ggcggtctcg aatgccgagc catgcagctc attggttccg
     4621 ctcttggtgg acatgataac agtggtcgga cgcgccagct tggcagagga gacattgctt
     4681 tccaccctga actcctggat ggtgtccaag cccgccggcc gccggaatac tgctcccgac
     4741 aagcggtctg tcgtggcggc gccgtcgagc acaaactcgg tcgatccccg tttcatcccg
     4801 aaagcgcgtg cggtcgtcga atccagatcc gatccatcca gttctaagcc ggccacggtt
     4861 tgcaggagcg cggtaatgaa acggccgttg atcggcaact gctcaatgcg ctcccgctcc
     4921 agcacgtggc tcaatgtcgg gctatccgcc gtgatcaacg gggtcacgtc ctggacagtg
     4981 acctccgtgg ctgtttgtcc caccttcagc acgacatcga caacggcgct ctgctgcacc
     5041 tgcactgtca gtttcccttc gaatttctgc atccccggcg cttccaccag aatctcgtag
     5101 gggccgggca cgatgccaag gaagaagtag attcctactg tggttgtcgt cgtcttggaa
     5161 acgatgttcg tagcggagtt cgtgagcgtt accgatgccg tcgggaccac ggcgccggat
     5221 tgatcgcgca ccgttccgct gactgtcgcg gtgctcgcct gcggccacgc cgtaggcgcc
     5281 agcaacgc
//
LOCUS       DATIFQ010000470         4031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_542406, whole genome shotgun sequence.
ACCESSION   DATIFQ010000470 DATIFQ010000000
VERSION     DATIFQ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542406"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VLE22_23615"
     CDS             <1..255
                     /locus_tag="VLE22_23615"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HSB17458.1"
                     /translation="GGAGQVFRFDFDQAQLAQAWFSGTGFGNNRIGVSAAVTDTAGSL
                     ERFYPYQLSPLPVPEPASFLTMGSGLVALGLLLRRYAKRS"
     gene            complement(322..1578)
                     /locus_tag="VLE22_23620"
     CDS             complement(322..1578)
                     /locus_tag="VLE22_23620"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB17459.1"
                     /translation="MKITRVQSHLLSYAFPEPIRLTYHGGERTILKRDALLIRIDTDE
                     GLTGYAPGQASEKAHAAINTTIASFLEGRALSDRDALRVLFLDGPGAEPEMAKVYAAV
                     EVGLYDLWGKAKGVPVSELIGGRVRDRIRLYGSAGMYMTPEGYAAEAQAVAKMGFAAY
                     KMRPGRGPETDLETVRLMREAAGPDVDLMVDAHTWWRMGDRSYSRSTVEQLAEALADY
                     GVAWLEEPLPPDDHEAYRRLREKDFVPLASGEHEPSEARFMDLIMNRAVDYVQMDLCC
                     QGGYSAGRRILLELAREGLGFAFHSWGTALEVIAAAHLGICWPEAVTGWLEYPCYSSP
                     GRPGMYPFPLASEILKTPLSILNGDLVVPATPGLGVEVDDTVIERYPWIPGPWSFFRL
                     DSPAETWAVTSDHSVKWSEKIPESGS"
     gene            complement(1753..2724)
                     /locus_tag="VLE22_23625"
     CDS             complement(1753..2724)
                     /locus_tag="VLE22_23625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17460.1"
                     /translation="MKAETVSIRESTGRVLCCTIFRQGGKKLLAKGHLISEEDVRLLE
                     TEGMENVWVTRLEDGEVGEDDAVMAVASEIGCGSLEIRLAAGGRANLVANEDCCVLVD
                     EDLLRQINCTSTTVIATAANFSFARTAQRIATVKSSPFAVAQTELEAVISILKERGPI
                     LQARPVRDPAVGVLYTDPVSGERARQLFDNVMRQRLERFDVAPHFTLARVEEEAAVAR
                     ALEYLLAARASVILVASTTAPAGPEDVVGQSLQRIGCHLERFLAPVEPGNLLLLAYKN
                     NVPIIAAPGCFRSAKANVVDLLLPAVLASYRISGWEVACLGHGGLLA"
     gene            complement(2904..3461)
                     /locus_tag="VLE22_23630"
     CDS             complement(2904..3461)
                     /locus_tag="VLE22_23630"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HSB17461.1"
                     /translation="MTEELVLRKALLTDLGPLLRLINDYASQGIMLPRTEFELSEGIR
                     DFTVALLDGRLVGCGALHFYGPHTAEVRSLAVEPDLKGHGVGRRLVEELENEAVVNGL
                     SSVFAFTYVPDFFHRLGYGEVDRSGLPGKVWKDCLRCAKFQCCDEIAMRKVLGVSPLE
                     DGTAPICGVDDNGVVQLPTIRKEML"
     gene            complement(3464..>4031)
                     /locus_tag="VLE22_23635"
     CDS             complement(3464..>4031)
                     /locus_tag="VLE22_23635"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HSB17462.1"
                     /translation="VLKVFAGSVNKQLVASLIQAGARAVGLSGIDAGLVEAEPLREEL
                     GAVGKPVRSDPDLLNLLTVAGYMPVVACVAGDRRGRIYNVNADQMAVACAAGFSAEVL
                     VFLTDVDGVLGPDKNRIPHLTSSGAEQLIRDGHASGGMQAKLNSSVEALRLGIKQVII
                     APGSAQNGVKTILNGALLGTRVTLFEAE"
BASE COUNT          772 a         1292 c         1192 g          775 t
ORIGIN      
        1 ggcggtgccg ggcaggtatt ccggttcgat tttgaccagg cgcagctcgc gcaggcgtgg
       61 ttcagcggca cgggctttgg aaataaccgg atcggagtct ccgccgcggt gacggacacg
      121 gctggcagtc tggagcgctt ctacccgtac caactcagcc cactgccggt gccagaaccc
      181 gcgtccttcc tgacgatggg ttccggattg gttgccttgg ggctgttgct ccggcgctac
      241 gcgaagagga gctagtttcg gtccttgaaa agtcggaatt gggcttaaag ggcgcggcca
      301 gaggtcgcgc ccttcggctt ttcaggagcc actttccggg atcttttcac tccatttcac
      361 actgtggtct gacgtgacgg cccacgtttc ggcgggagaa tcgagccgga agaacgacca
      421 gggccccgga atccacggat accgctcgat caccgtgtca tccacctcca cgccgagccc
      481 gggcgtggcc gggacgacaa ggtctccgtt cagaatggac agcggcgtct tcaggatctc
      541 ggacgccagc ggaaacggat acattcccgg tctgcctggt gacgagtagc aggggtattc
      601 cagccaccct gtgacggcct cgggccagca gatccctagg tgggccgcgg cgatgacctc
      661 cagagcggtg ccccagctat gaaatgcgaa gcccaggccc tcccgcgcga gctccaaaag
      721 gatgcggcgg cccgccgagt agccgccttg gcaacaaaga tccatctgga cgtaatcaac
      781 ggcgcgattc atgatcaggt ccatgaaacg ggcctccgaa ggctcatgtt caccgctggc
      841 gagcggcacg aagtctttct cccgcagcct tcggtatgcc tcatggtcat ccgggggcaa
      901 cggctcttcg agccaggcca caccgtagtc cgccaaggcc tcggccaact gttccacagt
      961 ggaccggctg tagctgcggt cgcccatgcg ccaccaggta tgggcgtcca ccataagatc
     1021 gacgtcggga ccggccgcct cccgcatcag gcgcaccgtt tcgagatccg tctccggtcc
     1081 gcggccgggt cgcatcttgt acgcggcaaa acccatcttc gcgaccgcct gcgcctcggc
     1141 ggcatacccc tccggcgtca tgtacatccc agcgctgccg tacagccgga tgcggtcgcg
     1201 cacccggccg ccgatgagtt ccgacaccgg cacgcccttg gctttgcccc agaggtcgta
     1261 tagcccgacc tcgaccgcgg cgtacacctt cgccatttcc ggctccgccc cgggaccgtc
     1321 gaggaacagg acccgcaagg catcccggtc actcagcgct cttccttcca gaaaagaggc
     1381 gatcgtcgtg ttgatcgccg cgtgcgcctt ctccgaagct tggccgggag cgtatccggt
     1441 caacccttca tcggtgtcga ttcgtatgag cagcgcatcg cgcttcagaa tcgtgcgctc
     1501 gcccccgtgg taggtgagcc ggatcggttc ggggaaagca tacgacagga ggtgggactg
     1561 aacgcgcgtg atcttcatgg tcgacgggcg gctccgggcg ccagctatca gcattcagct
     1621 tcggcgcttg tcctgtcaag actcctggga agttacgcac aaaaagagcc gggcgttggt
     1681 cagacgcccg gctgaggagc acccacaagc cactcggagg atatggcttg tggtagtgga
     1741 ggagacgaaa cgttaggcga gaagccctcc gtggcccaga caggccactt cccaccccga
     1801 aatccggtag ctcgccagca ccgccggcaa caaaagatcg acgacattcg ccttggcgga
     1861 acgaaaacaa cccggcgccg caatgatcgg cacgttattc ttgtaggcca gcaggagcag
     1921 gtttcccggt tccaccggcg ccagaaagcg ctcgaggtgg cacccgatcc gttgcaggga
     1981 ctgccccacg acgtcttccg gcccggccgg cgctgtcgtc gacgctacca gaatgacgct
     2041 cgctcgagcc gccagcagat attccagcgc ccgcgcgacc gcagcttctt cttcgactcg
     2101 cgccaacgtg aagtggggcg ccacgtcaaa ccgctctaag cgctggcgca tcacgttgtc
     2161 aaacaactgg cgcgcgcgtt caccgcttac gggatccgtg tacaaaaccc ctaccgcggg
     2221 gtcgcgcaca ggacgggcct gaagaatcgg cccgcgctct tttagaatcg agattacagc
     2281 ttccagctcc gtttgcgcaa cggcaaacgg cgaacttttt acggttgcga tgcgctgcgc
     2341 cgtccgggcg aagctgaaat tggcagccgt cgcgatcacc gtggtggacg tgcagttgat
     2401 ctggcgcaag aggtcctcgt caacgagcac gcagcagtcc tcgttggcga ccagattggc
     2461 tcggccaccc gccgccaggc ggatttccaa agagccgcag ccgatctcgg aagcgactgc
     2521 catcactgcg tcgtcttccc cgacctcgcc gtcctccaat cgcgtaaccc aaacattctc
     2581 catgccttct gtctcaagaa ggcggacatc ctcctcgctg atgagatggc cttttgccag
     2641 cagctttttc ccaccctgcc ggaagatcgt gcaacacaac actcggcccg tggattccct
     2701 gatggagact gtttccgctt tcatcctggt tgcgtggtca ctggcgccac gcgcgccggc
     2761 atcaaatgga ttatacgcag ggatagtgat tccgggagta ggtctttctc agctagcagc
     2821 caagtctttt gagggtgcgg agtcgctccc tttgagggga gagaatctct agtgatatct
     2881 tgcgaggagt gcggattttt ccatcagagc atttcttttc ttatagttgg caattgcaca
     2941 acgccgttgt cgtctacccc gcaaataggg gcagtaccgt cttcaagcgg actaaccccc
     3001 agtactttcc tcatggcgat ctcatcacag cactggaact tcgcgcagcg aaggcagtct
     3061 ttccagacct ttccggggag cccgctccgg tccacctctc catatcccag gcggtggaag
     3121 aagtcgggga cgtatgtaaa cgcgaacacc gaggaaaggc cgttcacaac cgcttcattt
     3181 tcaagctctt ccacgaggcg acggccgacg ccgtgcccct ttaggtccgg ttcgacggcg
     3241 agtgagcgga cctccgcggt atgcgggccg tagaagtgca atgctccgca gcccaccagc
     3301 cgcccatcga gcagcgcaac ggtgaaatcc cgaatgcctt cagagagttc gaactcggtg
     3361 cgggggagca tgatcccctg cgaggcgtag tcgttgatga ggcggagcag cgggccgagg
     3421 tccgtgagga gggctttcct cagcaccaat tcttcagtca tggctactca gcctcgaaaa
     3481 gggtcacgcg agtgcccaaa agtgcgccat ttaggatcgt tttgacgcca ttttgggcgc
     3541 ttcccggcgc gatgatcacc tgttttatgc ccaaccggag ggcctccacg gaggaattca
     3601 gcttggcttg catcccgccg ctggcgtggc cgtccctgat gagctgttcg gctcctgacg
     3661 aggtcaggtg tgggattctg ttcttgtccg gtcctagaac tccgtccaca tccgtaagaa
     3721 aaacaaggac ttccgcggag aatccggctg cacaggcgac cgccatctga tcggcgttta
     3781 cgttatagat ccgcccacga cggtccccgg ccacgcacgc cactactggc atatacccag
     3841 caactgtaag taaattaagg agatctggat ccgagcggac cggttttccg accgcaccga
     3901 gttcctcgcg caatggttcg gcctcgacga ggccggcgtc gatgcccgaa agaccgacgg
     3961 cccgcgcccc ggcctggatg agcgatgcca ccagttgttt gttgacgctg cccgcgaaaa
     4021 ctttcaagac g
//
LOCUS       DATIFQ010000471        10843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2457474, whole genome shotgun sequence.
ACCESSION   DATIFQ010000471 DATIFQ010000000
VERSION     DATIFQ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2457474"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VLE22_23640"
     CDS             complement(<1..450)
                     /locus_tag="VLE22_23640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17463.1"
                     /translation="MSVLRFQTNVPVEVALAYSDGKEIEGQYGPQVMFSLAESPNGEK
                     TMYVPPIVAERFRELHIDKGQRVRVCKDEVKNGNRRGIQWDVSRIDPDPRWEHPAPGQ
                     PAAPPPGMVVGAAVPVRNAGSDPSNGQSTTPVNGNGHATANGVPYWDP"
     gene            complement(447..923)
                     /locus_tag="VLE22_23645"
     CDS             complement(447..923)
                     /locus_tag="VLE22_23645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17464.1"
                     /translation="MSTAPLLSTIERRQAYRLGRLSYDLGKAQAEASEHQARAFEAER
                     LIEALGDVLDLSTPTLQEIGRADPNYFTEPREKLLEALKAWGEDRKIPVCLACGSPRV
                     DWIEWGGATPAPDGGAEARGCGWCCQDCGAIEDTTGEGRRATTSFAVNDQTKGVSL"
     gene            complement(920..1270)
                     /locus_tag="VLE22_23650"
     CDS             complement(920..1270)
                     /locus_tag="VLE22_23650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17465.1"
                     /translation="MNSGYDAWKLNPPLTNFAGRCPTCRAEYEFDSRPQVDEDGMPDY
                     GVSTRPCVGCGVELCEACPQKRREQCRAPLCEVCAIHEPRNPAEFCLTCCLELVAEWA
                     AARAARKEGKEITQ"
     gene            complement(2225..2300)
                     /locus_tag="VLE22_23655"
     tRNA            complement(2225..2300)
                     /locus_tag="VLE22_23655"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2265..2267),aa:Lys,seq:ttt)
     gene            2484..3998
                     /locus_tag="VLE22_23660"
     CDS             2484..3998
                     /locus_tag="VLE22_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17466.1"
                     /translation="MAAIRREGREVKRGHPFQPEKGHGFIDSQKYGMEVFRQIPPDAP
                     LVVVEAVWQYSHHVFHGLYSHKGPILTVANWSGEWPGLVGMLNLNAGLTKAGVRYSTL
                     WSEDFTDDFFLHGLRKWLAGEQVVHDTSHVRPLRGLKLPAQAEQIGAELARELLVNKA
                     IMGIFDEGCMGMYNAIIPDELLHATGIFKERLSQSALYAAMLAVPKEEAQAVRTWLDN
                     KGMKFLTGPNPESDLTDGQILDQCRMYIAALRIADDFGCDTIGIQYQQGLKDLAPASD
                     LAEGLLNNVDRPPVRHAVTGKILYEADALPHFNEVDECAGLDALVTNRVWKKLGFPPE
                     TTLHDVRYGETWRSNGSEQFVWVYEISGAAPPAHFIGGYADAVSERQPAMYFRLGGGS
                     LKGVSKPGEIVWSRVFVDNGALKADLGRAEVIELPDEETQRRWKITTPQWPVMHAVTY
                     GMTRDQFMARHKSNHIQVAYAPDADGANLALAAKAAMFREMGLDVSVCGTNHGL"
     gene            4023..5249
                     /locus_tag="VLE22_23665"
     CDS             4023..5249
                     /locus_tag="VLE22_23665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17467.1"
                     /translation="MPTRCTLSAVLLLAAWPLVAGEITEKNLRFAEYPDFPDAHSTWG
                     SIGYSAKHKKVFVGVTNHRDREGIVEYDVRTGEIRLRGFIDQLANLRDYQWQGKVHTK
                     LAEGPDGAMYFATDGGESREEYLMDHPHGYGGGFFFRWDPATNQLTNLGMGLQYDSIK
                     DVEVDQVDGKIYAVSYPQVRFLVYDFQKNDLRDLGRMGSDHVPRILFRDWWSNAYYVD
                     WRQRLIKYERDGGKLVFARDSLPAFPGTPGERIITGITAYAIDQPNGIIYLATYGSKM
                     VAFYPQKTGIGRWEDLGGLYDDPGKPPYDYYVPNLALGRNGKLYYFLGGHGMYAGGEP
                     NVTLMEFDPKQRTKRVVLRFPLHVITEVTGCDVKDESGTMYFAGRRDDRRARRMGESG
                     ASRPFLLMFNPEKEIR"
     gene            5249..6427
                     /locus_tag="VLE22_23670"
     CDS             5249..6427
                     /locus_tag="VLE22_23670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17468.1"
                     /translation="MRRTSLIALVLAAASLVWARYEFFTPKGSFAVEVSLDNAPYLRL
                     PIYRNAITSLEVIGDYAIGGTSAESGLRPFLFAVSLSKRKLEIVYPLEKTIPGQRAIQ
                     SGFGRGADGTLYAGTMPDRPGESGHLIAVRVKAGAIDVIDLGVPVKGEGVFTLTFDPK
                     AGALYGISHPSGKFFVSRPNGGAAEVYEQTAPNRRGFSFLHEYALEPADVLSRRLAID
                     RSGRVYGSMPANKLFRFDSAAKKVDVLPDPLPEVWGRSPLGRVDAWAVASDGTLYGGN
                     AGDGQLFKLDPATGKVTNLGKAIMMPRLKGIVFARDGRLYGIAGGAPGYSHLFSYSPS
                     EGYRDLGNPRFPMVEPDLEQEIPWRGFQLGTLAVSEDGRYVVMGEEEALSQLMVFPVE
                     "
     gene            6539..7555
                     /locus_tag="VLE22_23675"
     CDS             6539..7555
                     /locus_tag="VLE22_23675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17469.1"
                     /translation="MRVGIIGTGAISDKHALAYRNIGYEVTVCTDIREEAGRRFAEKY
                     GVEYVRTYEEVCRHPKVDFVDLCTFPDFRLQPIEICAETKKHIQVQKPISTNIETARK
                     MIDTAKKAGITLGVVSQHRFDDSSIFLSKAIAAGRMGKLIQADAYVKWYRSAEYYSRP
                     IKGSWATEGGGALINQAVHQVDVLLWLIGPVKELFAFWQLGALHKIESEDVITTTMRY
                     ANGANGVIQASTAMWPGYSERIEIHGTKGSAILTGDKLTAWDVQDDSGEPAPVEKEVM
                     SGSSDPMAIPLTPFERQFLNFGDAIKTGRKPLVSGEDGYRALEFVTSAYRSAREGRKV
                     TLGE"
     gene            7548..8477
                     /gene="thrB"
                     /locus_tag="VLE22_23680"
     CDS             7548..8477
                     /gene="thrB"
                     /locus_tag="VLE22_23680"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HSB17470.1"
                     /translation="MSSPAPQMPWTKVRVPASSANLGPGFDALGLALGVYLECRFRPS
                     ETLVIRACGRDANQISTGEDNLIWQTALAVAEGRQAELPAIELEIDNRIPIGKGLGSS
                     AAALIAGVVIADKILGLNWKRPRILDEAARIEGHPDNVAACVLGSIVASAIDSTGVAR
                     AVRIELPKRFGVAVVVPDFILPTTKARAVLPDSYSKQDAIFNVQRASLLIAALAMGVA
                     SAFPATLEDRFHQPFRERLVPGLGEILRLRAPGLLGCALSGAGPSVLVFYERGYEGVC
                     DLVRQVFRLHGHASEVLWARIDDHGYEVHQEEQ"
     gene            8479..9411
                     /locus_tag="VLE22_23685"
     CDS             8479..9411
                     /locus_tag="VLE22_23685"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HSB17471.1"
                     /translation="MDASRKFLKGLGLPGADPHDCPDSPKRFPDGGQYRIEIPSTEGP
                     RCLAAVLDEASKRKVPVHRVSQGSGILLMTDEEITEMLAIGREAGIEVNLFIGPRATF
                     DIGAQAYSTAGKALGLSLRGADQLVYAMLDVKRAVRLGLRSILVSDLGILQIIAKMRA
                     AGELPADLIIKTSVMMAPANPASTRILEVLGADTINIPSDLTLPQIAAIREAISVPID
                     FYVEAPDNIGGFIRHYEIPELIRVAAPIYLKFGLKNAPDVYPSGSHIENTVIALSRER
                     VRRAEIACDIITRYCPDAVMSPLRAKDLGIPAAV"
     gene            9655..10140
                     /locus_tag="VLE22_23690"
     CDS             9655..10140
                     /locus_tag="VLE22_23690"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB17472.1"
                     /translation="MASPFARTNPAYEELHRSCQADWPAVIVVDTNVVAYALIEGAHT
                     PLALQVRKIDPAWRLPDLWRHEFLNILATYVRQGGTDENTARRLWLKADDLLAPATVA
                     VDLSLALGLAVRYRISAYDAQFIALASTLGVPCVTEDRALQKAFPGSAVSMREFCRHL
                     E"
     gene            10152..>10843
                     /locus_tag="VLE22_23695"
     CDS             10152..>10843
                     /locus_tag="VLE22_23695"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB17473.1"
                     /translation="MPQTFIPVLHSVSYAGAWPGTALLSVDAFLDKAAELGFRHIALV
                     AKRPHASPFDFDAAARKALKKRIRDLGLTLEALMGYTDFTAGIERPGIPSAEMNAGYV
                     EVLSQLAADLETKRLRIFTGYERHGVSYDAQYIELVKGLRLASRAAAKYGVTLLVQNH
                     HDLAVHHDQIAWMLKELSEPNCKVAFDAWAPFLQGLSGQAMVDAVKTVGPWIAWTTVA
                     DYVKHPRFKYDA"
BASE COUNT         2018 a         3597 c         3255 g         1973 t
ORIGIN      
        1 cggatcccag tacgggaccc cgttggccgt ggcgtggccg ttgccgttta ccggcgtggt
       61 agactggccg ttgcttggat cacttccagc gttccggacg ggaaccgcgg cgccgactac
      121 catgccagga ggaggcgctg cgggctgtcc gggagccggg tgctcccagc ggggatccgg
      181 atcgatccgg gacacatccc actgaatccc gcgccggttc ccgttcttga cctcgtcctt
      241 gcagactcgt acccgctggc ctttgtcgat gtgtagctcc cggaaccgct cggccacgat
      301 gggcgggacg tacatcgttt tctcgccgtt gggggactcg gcgaggctga acatgacttg
      361 agggccgtac tgcccctcaa tctctttccc gtcgctgtag gccagggcca cttcgacggg
      421 cacgttggtt tgaaatcgca acacgctcat aagctgactc ctttcgtctg atcgttcact
      481 gcaaaactcg ttgtcgccct ccgtccctca ccagtcgtgt cctcgatcgc gccgcagtcc
      541 tggcagcacc agccgcagcc ccgcgcttcg gctcctccgt ccggagccgg ggtcgcgccg
      601 ccccactcga tccagtccac ccgcggcgaa ccgcaggcga ggcagaccgg gatcttccgg
      661 tcttcgcccc aggctttgag ggcctccagg agtttctcgc ggggctccgt gaagtagttc
      721 gggtcggctc ggccgatctc ctgaagcgtc ggggtgctca gatcgaggac gtcgcccagc
      781 gcctcgatga ggcgttccgc ctcgaacgcc cgcgcttggt gctcactggc ctcggcctgg
      841 gccttaccca ggtcgtagct gagccggccg agccggtagg cttggcggcg ctcgatagtg
      901 gacaacagtg gtgccgtact cattgggtga tctcctttcc ttcctttcgc gccgccctcg
      961 cggccgccca ttcggccacg agctccagac agcaggtcag gcagaactcg gccgggtttc
     1021 gcggttcgtg aatggcgcag acctcgcaca gcggcgcgcg gcactgctcg cggcgcttct
     1081 gcgggcaggc ctcgcagagt tcaacgccgc agcctacgca gggccgggtg gagaccccgt
     1141 aatccggcat cccgtcctca tccacctgcg gtcgggagtc gaactcgtat tccgccctgc
     1201 aggtggggca gcggccggcg aagttcgtca ggggtgggtt cagcttccaa gcgtcgtagc
     1261 cggagttcat cgtgacgtat ccatgacaaa gcgcattgtc atgcttacga atatagcata
     1321 acaatgcgta ttgtcaagca taattgttcg taccattgga cgtactggca cccgcccccg
     1381 agcactgcgg cgaaagaaat gcaaaagaaa tgcccttaac cttgacaagg cgctgcgtta
     1441 tgctatacgc gtgaggaaaa acccccacgc cgtagctttg gggagacgcg gcggtcgatc
     1501 acgaatggct tcgatgtctc ctgaggaact ccaggaattc gttcgcagag gtggcgaggc
     1561 aggcggaaag gccagagccg cgacgctgtc tgcgaaacgc cgatccgaga tcgcccgcaa
     1621 ggcggcccag gcgcggtggg ggaagccgaa gccctgagtg acggaatcta cgaagccagg
     1681 gtaggcagtc gtagagctac tcgcctgaag ggatacggcg gcagatcgaa ccttagatgg
     1741 gagggctcag ccacgccccc ctgccacttc attgagagca tcttctctgg tggcctccgg
     1801 atacccgtag acttgcccgt tttgatcgag gacaatccat tctttgcccg gctgttcaac
     1861 gcgccaacct tccggcagtt gccccggcac tactgcgtac ccttctcccc tgcttggtgc
     1921 tggtcgctct gctcctcgtc gatcagcagc gcccacttgc gttcgaagtt ccggcgcgaa
     1981 gaggttgacg catgcgtgaa gagcgggccc ggcgcgatct ggagaagtag gcgaaatgca
     2041 gacgcagaaa gcagtcaagc gaattcggca gcggttgtca cgggtatcgg accttcacaa
     2101 ccgcctgctg ctcgtcattg acgtcagcgc gttggcacgg aggagcggcc ccccccaagt
     2161 attagtatcg cccgaagtat cgcccgaccg tattcgaccg atcgtagaaa tcctttgttt
     2221 cgaatggtga gccggccggg actcgaaccc gggaccctct gcttaaaagg cagatgctct
     2281 accgtctgag ctaccggctc ttcgagactg caattggcgg gtcttccacg aggataacac
     2341 agcgcggcaa atcgtctgtt cacctcgagt tggcctagaa taagaccatg ccggaaccag
     2401 tcatccttat cgctaatgga gatttgcggc tatccgccaa ccagatctgt tgggcggctc
     2461 agaacggcgc cgaacaggcg gtcatggccg ccatccgccg cgagggccgc gaagtcaagc
     2521 gcggccatcc cttccagccc gagaaaggcc acggattcat cgattcccag aagtacggca
     2581 tggaggtttt ccgtcagatc ccgcccgacg cgccgctcgt cgtcgtcgag gccgtctggc
     2641 aatacagcca ccacgtcttc cacgggctct attcccacaa aggtcccatc ctcaccgtcg
     2701 ccaactggag cggcgaatgg ccgggtctcg tcggtatgct gaacctcaac gcgggactca
     2761 ccaaagccgg cgtccgctac agcacgctct ggagcgagga cttcaccgat gacttcttcc
     2821 tccacgggtt acgtaagtgg cttgccggcg agcaggtggt ccacgatacc agccacgtcc
     2881 gcccgctgcg cggcctcaaa ctgcccgccc aggccgaaca gatcggtgcc gaactcgccc
     2941 gcgagctgct cgtcaacaag gccatcatgg gcatcttcga cgaaggctgc atgggcatgt
     3001 acaacgccat catccccgac gagctgctcc acgcaaccgg catcttcaaa gagcgcctca
     3061 gccagtctgc tctctacgcg gcgatgctcg cggtcccgaa agaagaggcg caagcggtcc
     3121 gcacatggct cgacaacaag ggcatgaagt tcctcaccgg cccgaacccc gagagcgatc
     3181 tcaccgacgg ccagatcctc gaccagtgcc gcatgtacat cgccgccctg cgcatcgccg
     3241 acgatttcgg ctgcgacacc atcggcatcc agtaccagca ggggctgaag gatctcgcac
     3301 ccgcctccga tctcgccgag ggcctgttga acaacgtcga tcggcctcct gtccggcacg
     3361 ccgtcacggg caagatcctc tatgaagccg atgccctgcc ccacttcaat gaagtggacg
     3421 agtgcgccgg cctcgacgcg ctggtgacca accgcgtctg gaagaagctc ggcttcccac
     3481 ccgagaccac gctccacgac gtccgctacg gcgagacctg gcgctcgaac ggcagcgagc
     3541 agttcgtgtg ggtctacgaa atctcaggcg ccgcgccgcc cgcgcacttc atcggcggct
     3601 atgcggacgc cgtcagcgaa cggcagcctg cgatgtactt ccgcctcggc ggcggcagtc
     3661 tcaagggcgt cagcaagccg ggcgagatcg tctggagccg cgtcttcgtg gacaacggag
     3721 cgctcaaggc cgacctcggc cgcgccgagg tcatcgagct tccggacgaa gagacccagc
     3781 gccgctggaa gatcacgacg ccacagtggc cggtgatgca cgcggtgacg tacggaatga
     3841 cccgcgacca gttcatggca cgccacaagt cgaatcacat tcaagtggcc tacgcgccgg
     3901 acgccgacgg cgccaacctc gcgctcgccg ccaaggccgc catgtttcgc gagatggggc
     3961 tcgacgtcag cgtttgcgga acgaaccacg gtctgtagca ggtacactca cacaacactc
     4021 ctatgcctac acgatgcact ctatctgctg ttctcctgct ggccgcctgg ccgctagtgg
     4081 caggcgagat cactgagaag aatcttcgct ttgccgaata tcccgatttt cccgacgccc
     4141 attccacatg ggggtccatc ggctatagcg cgaaacacaa gaaagtattt gtcggcgtaa
     4201 cgaatcatcg ggaccgcgag ggtatcgtcg agtacgacgt ccgtactggc gagatccgcc
     4261 tgcgcggttt catcgaccag ctcgccaacc tgcgcgacta ccagtggcag ggcaaggtcc
     4321 acaccaaact cgccgagggc ccagacggcg cgatgtactt cgccacggac ggcggggaga
     4381 gccgcgagga atacctcatg gaccatcccc acggttacgg cggcggcttc ttcttccgct
     4441 gggacccagc aacgaaccag ctcaccaacc tcggcatggg cctccaatac gacagcatca
     4501 aggacgtcga agtggaccag gtggacggca aaatctacgc cgtctcctac ccgcaggtcc
     4561 gcttcctggt ctacgatttc caaaaaaacg atctgcgcga cctcggtcgc atgggcagcg
     4621 atcacgtccc ccgcatcctg ttccgcgact ggtggagcaa cgcctattac gttgactggc
     4681 ggcagcggct catcaagtac gagcgcgacg ggggtaagct ggtcttcgcg cgggatagcc
     4741 ttcctgcctt tcccggcaca ccgggcgagc gcatcatcac tgggatcaca gcctacgcga
     4801 tcgatcagcc gaacggcatc atttacttag cgacgtacgg ctcaaagatg gtcgcgttct
     4861 acccgcagaa gaccggcatc ggaagatggg aagacctcgg cgggctctac gatgatccag
     4921 gcaagccgcc ctacgattac tacgttccga acctcgcgct cggccgcaat ggcaagctgt
     4981 actatttcct cggcggtcac ggcatgtacg caggcggaga gccgaacgta accctgatgg
     5041 agttcgatcc aaagcagcgg accaagcgcg ttgtcctccg ctttccgctc cacgtgatta
     5101 ccgaggtcac cggctgcgac gtcaaggacg aaagcggcac gatgtacttc gccggccgcc
     5161 gtgatgaccg gcgcgcgagg cgaatgggag aatccggagc gagccgcccg ttcctgctca
     5221 tgttcaaccc cgaaaaggag atccggtaat gcgccgaacc agcctgattg cactcgtcct
     5281 cgcagccgcg tcgctcgtat gggcgaggta cgaattcttc acaccgaagg gcagcttcgc
     5341 cgtcgaagtg tcgctcgata atgcgcccta cctgcgcctg ccgatctacc gcaacgccat
     5401 cacttcgctc gaggtgatcg gcgattacgc gatcggcggc acgtcggcag agtccggcct
     5461 caggccgttc ctgttcgcgg tctcgttgtc caaacggaaa ctcgagatcg tctatccgct
     5521 cgagaaaacc atccccggcc agcgcgccat ccagagcgga ttcggccgcg gggcggacgg
     5581 cacgctttac gccggcacca tgcctgaccg gcccggcgag agcgggcatc tcatcgccgt
     5641 tcgcgtcaag gccggcgcga tcgacgtcat tgacctgggc gtgccggtga agggagaagg
     5701 agtgttcact ctcacgttcg atccgaaggc gggcgcgctc tacggcatca gccacccatc
     5761 cggcaagttc ttcgtgtccc gcccgaacgg cggcgccgcg gaagtctatg agcagaccgc
     5821 tcccaaccgt aggggcttca gcttcctgca cgagtatgcg ctcgaaccgg cggacgtcct
     5881 gagccggcgg ctggcgatcg accgctccgg tcgtgtctac ggcagcatgc cggccaacaa
     5941 gctctttcgc ttcgactcgg cggcgaaaaa ggtcgacgtg cttcccgatc ccctgccgga
     6001 agtctggggg cgcagtccgt tgggccgtgt ggacgcatgg gccgttgcct cggacggcac
     6061 gctgtacggt ggtaacgccg gggacgggca gctcttcaag ctggacccgg ccacgggaaa
     6121 ggtgactaac ctcggcaaag cgatcatgat gccccgcctg aaaggcatcg tgttcgcgcg
     6181 cgatggcagg ctgtacggca ttgccggcgg cgcgccgggc tactctcacc tgttcagcta
     6241 ctcgccgtcg gagggttacc gggatctcgg caatccgcgc tttccgatgg tcgagcccga
     6301 cctcgaacag gagatcccgt ggcgcggctt ccagttgggc acgctggcgg tctccgaaga
     6361 cggccgctac gtcgtgatgg gcgaggaaga agccctcagc cagttgatgg tcttcccggt
     6421 ggagtaaggc atttgtagcg gctcacgtgg ggcggatgat cgcatttcgt cgtccgccca
     6481 ccaggcacag ccctcaggct gtgctgcgtt atacttccat cttcaaggag acactgcatt
     6541 gcgagtcgga atcatcggta ccggcgcaat ctcggataag cacgcgctcg cgtacaggaa
     6601 catcggatac gaagtcaccg tctgcacgga tatccgcgag gaagccggcc ggaggttcgc
     6661 cgaaaagtac ggcgtggaat atgtcaggac gtatgaggaa gtctgccgcc atccgaaggt
     6721 ggacttcgtc gatctctgca ctttcccgga tttccgcctc cagccgatcg agatctgcgc
     6781 ggagacgaag aagcacatcc aggtccagaa gcccatctcg accaacatcg agaccgcgcg
     6841 caagatgatc gacacagcga agaaggccgg catcaccctc ggcgtcgtca gccagcaccg
     6901 tttcgacgac tcgagtattt tcctgtcgaa agccatcgcc gcagggcgca tgggcaagct
     6961 catccaggcc gacgcctacg tgaagtggta ccgctccgcc gaatattact cccggccaat
     7021 caagggcagt tgggccacag agggcggcgg cgcgctgatc aaccaggcgg tccaccaggt
     7081 ggacgtgttg ctctggctca tcggtcccgt gaaggaactc tttgcgttct ggcagcttgg
     7141 cgcgttgcac aagatcgagt ccgaggacgt catcacaacc acgatgcgct acgcgaacgg
     7201 cgcgaacggc gtgatccagg cctccaccgc catgtggccg ggctacagtg agcggatcga
     7261 gattcacggc accaaaggct cagccatcct gaccggtgac aagctcaccg cgtgggacgt
     7321 ccaggatgac tccggcgagc ccgcgcccgt cgagaaggaa gtcatgtcgg gctcttccga
     7381 tcccatggcc atcccgctta cgccgttcga gcgccagttc ctcaatttcg gcgacgcgat
     7441 caagaccggc cgcaagccgc tggtctctgg cgaggacggc taccgcgccc tcgagtttgt
     7501 gacttcggcg taccggtccg cgcgcgaggg ccggaaggtc accctgggtg agtagcccgg
     7561 ctccccagat gccctggacc aaggtccggg tcccggcttc cagcgccaat ctcggccctg
     7621 gcttcgacgc gcttggcctc gcgctgggcg tttaccttga gtgccggttt cgcccaagcg
     7681 aaacccttgt catccgggcc tgcgggcgtg acgcgaatca gatctcgacc ggcgaggaca
     7741 acctcatctg gcagacggcg cttgcggtcg ccgaaggcag acaagccgag ttgccggcta
     7801 tcgaactcga gatcgacaac cgcatcccga tcggcaaggg cctcgggtcg agcgcggcag
     7861 cactgatcgc gggcgtggtg atcgccgaca agattctcgg tctcaactgg aagcggccgc
     7921 gaattctcga cgaagcagct cgcatcgagg gccatcccga taatgtcgct gcgtgcgtgc
     7981 tcgggtcgat tgtcgccagc gccatcgatt ccaccggcgt cgcccgcgcc gtgcgcatcg
     8041 aactgccaaa gcggttcggc gtggcggtgg tggtgccgga tttcatcctg ccaacaacga
     8101 aagcccgcgc cgtcctgccg gattcctact ccaagcagga tgccatcttt aatgtgcagc
     8161 gcgcgtcgct gctcatcgcc gcgctggcga tgggcgtcgc aagcgcgttc ccggccacgc
     8221 tcgaggaccg tttccatcag cctttccgtg agcggctcgt gcctgggctc ggcgagatcc
     8281 tgcgattgcg tgcgccgggc ctgctcggct gtgcgctgag cggcgccggt ccgtcagtgc
     8341 tcgtgttcta cgagcgcggc tacgagggtg tctgcgatct ggtacgccag gtgttccgtc
     8401 tccacggcca cgcctccgag gtgctctggg cgcggatcga cgaccacggt tacgaagtcc
     8461 atcaggagga gcagtgacat ggacgcatcg cgaaagtttc taaaaggcct cgggctgccc
     8521 ggcgccgacc cgcacgactg ccccgattcg cccaagcgtt ttcccgacgg cggccagtat
     8581 cgcatcgaga tcccatcgac cgaggggcct cgctgcctcg ccgccgtgct cgatgaggcg
     8641 agcaagcgca aagtgccggt ccatcgcgtc agccagggct ctggcatcct gttgatgact
     8701 gacgaagaga ttaccgagat gctggccatc ggccgcgagg ccggcatcga agtcaacctc
     8761 ttcattgggc cacgcgccac tttcgatatc ggcgcccagg cgtattcgac cgccgggaag
     8821 gcgctcggcc tgagcctgcg cggggccgat cagcttgtct acgcgatgct cgacgtcaag
     8881 cgcgctgtac ggctcgggct ccgcagcatc cttgtttccg acctcggcat cctccagatc
     8941 atcgccaaga tgcgcgccgc gggtgagttg ccggcggatc tgatcatcaa aacttccgta
     9001 atgatggcgc ccgcaaatcc cgccagcacc cgcatcctgg aagtgctcgg agccgacacc
     9061 atcaacatcc cgtcggatct cacgctgccg cagatcgccg ccatccgcga ggcgatcagc
     9121 gtgccgatcg acttctacgt cgaagcgccc gacaacatcg gcggcttcat ccggcactac
     9181 gagatcccgg aactcatccg cgtcgccgcg ccgatctacc tgaagttcgg cttgaagaac
     9241 gcacccgacg tctacccgag cggcagccat atcgagaaca ccgtgatcgc actctcccgc
     9301 gaacgcgtcc gccgcgccga aatcgcctgc gacatcatca cccgctactg ccccgacgcc
     9361 gtgatgtcgc cgctgcgcgc caaggatctc ggcataccgg cggcggtatg attttggtcc
     9421 cctatgcagt taccgagctg cgactggagg gagcagaagc ccagcgccct gttacgattc
     9481 actagcatta tgctagtatt gtcatagcgc tatgcctggt ctgctgatca aggaattgcc
     9541 tccggcactc caccgtcgcc tcaagaaagc ggcggcgcag caccaccgga gcatgacgcg
     9601 cgaagcgctt tccattttgg aggagggcct cagccgctcc gagccttccg tcgaatggcc
     9661 tctccctttg cgcggacgaa tccggcttac gaagagcttc atcgatcgtg ccaagcggac
     9721 tggccggccg tgatcgtcgt tgatacaaac gtagttgcct acgcactgat cgagggcgct
     9781 cacactcccc tcgccttgca ggttcgaaag atcgacccgg cctggcgtct ccccgatctt
     9841 tggcggcacg agtttcttaa tatcctcgca acgtacgttc gccagggagg cacggatgag
     9901 aacacggccc gccgtttgtg gctcaaggcc gatgatctgc tcgccccggc aaccgtggca
     9961 gtggatctct cgcttgccct ggggctcgcc gtccgctatc gtatcagcgc gtacgacgca
    10021 cagttcatcg ccctggcctc gacacttggc gttccgtgcg tgaccgagga tcgcgcccta
    10081 caaaaggcgt tcccagggag tgcggtgtcg atgcgcgagt tctgccgcca tttagaataa
    10141 ggttcatact tatgcctcaa acgttcatcc ccgtccttca ttccgtaagc tacgccggag
    10201 cgtggcccgg gaccgcgctc ttgagtgtcg atgcgtttct cgacaaggcc gccgaactcg
    10261 gcttccgcca tatcgcgctg gtggccaagc gcccccacgc ctcaccgttc gactttgacg
    10321 ccgccgcacg taaggccctc aagaagcgca tccgcgacct cggcctcacg ctcgaagctt
    10381 tgatgggcta caccgacttc accgcgggca tcgagcggcc cggcatccct tccgccgaga
    10441 tgaatgccgg ctacgtcgaa gtgctgtcgc agcttgccgc cgatctcgaa accaaacgcc
    10501 tgcgcatctt cacgggttac gagcgccacg gcgtctctta cgatgcccag tacatcgagc
    10561 tcgtcaaggg tctgcgcctc gcgtcccgcg ccgccgcaaa atacggtgtc acactgctcg
    10621 tccagaatca ccatgacctc gccgtccatc acgaccagat cgcctggatg ctcaaggaac
    10681 tcagcgagcc caactgcaaa gtagcctttg acgcatgggc gccgttcctg caaggtctct
    10741 ccgggcaggc gatggtcgac gctgtgaaga ctgtcggccc gtggatcgcc tggaccaccg
    10801 tggccgacta tgtcaaacac ccgcgtttca agtacgacgc cca
//
LOCUS       DATIFQ010000472        48756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2265226, whole genome shotgun sequence.
ACCESSION   DATIFQ010000472 DATIFQ010000000
VERSION     DATIFQ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 48756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48756
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2265226"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VLE22_23700"
     CDS             <1..799
                     /locus_tag="VLE22_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17474.1"
                     /translation="QLRVAMPLGETPWQVIAVEGNARLGGGAAERAAKLRTGQHLRTD
                     GGKVTLEADEIGRIEVAAGSELRVTESTAAKQTLSLRRGLIHALIWAPPRRFVVDTPS
                     ARTIDLGCEYTLFVDDDGDGLIRVKTGWVAFQFDGRDSFIPAGAACRTTRRGGPGTPY
                     FEEASQELRRALAAFDASGNGGDLKQVLAEARPRDGLTLWHLLTRAAEADRGLVFDRF
                     ATFVALPAEVTREGVLAKDRRMLDLCWNALNLENAEWWREWKRAWQP"
     gene            1162..2061
                     /locus_tag="VLE22_23705"
     CDS             1162..2061
                     /locus_tag="VLE22_23705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB17475.1"
                     /translation="MSYVDRRQIFHAGLVSLANFVMGEPLTSEAKAETRRGIFDAHLH
                     IPSDNGENFQWYKVTKNMPEFVAYLDRCGVRRGVISSSWSNKAKTPDDYRNGNREVVK
                     YVEQYKGRFRGSCVITPFKIDEALREIETCAKQFGFVWVGECCNYMTGYKYETPEWTE
                     VMNLVTRLNLVVQIHTNTSEMQYLAEKFPDTTIVFPHPGGNLNDISARIAIIAKHKNT
                     HLELSGSGHERVGIVEKAVQEIGADRVLYGSDFTINEPSGVIMRVKNAFLTAQDREKI
                     LFRNLERLLAGAGPGRARQPKNT"
     gene            complement(2109..5021)
                     /locus_tag="VLE22_23710"
     CDS             complement(2109..5021)
                     /locus_tag="VLE22_23710"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17476.1"
                     /translation="MERLLEQVNFRDPVRAAAQIASVTQATPAHTAPKLRHLLAASPD
                     PDEVLHLLERMAVQHPDDFARITASDAGLTSLTAVFSYSRFLSEAILREPSWIEDTIG
                     ARDMDRTLSAEEYETRLENALGGDSGVLSPLTLAVFRRKQILRILLRDVLSFATLAET
                     VEELSNLADAILEVACRRIRAELVRRHGLPRCYGGDGEPLECGFAVISLGKLGGKGLN
                     YSSDIDLMFVYGAAGETDGPNRITNKEFYKKVANQLTDLLSTYTAEGVCYRVDLRLRP
                     DGRLGEVCISLDGARSYYRDRARDWELQMLIRARVSAGDQRLGRELLEFNEPLIYSST
                     LDFRAIESVSATRERISEKLAARRGSGFEIKLAPGGIRDVEFLVQCLQRLHGGREPWV
                     RLGGTLRGLSRLHDKGLLSGIEYSRLASAYQFLRYLEHRLQFDEDRQTHALPEDLRER
                     EVLARKMPPALIGEEPSAQALLARLNHHLEEVREIYERVIHAQQPIYYTLSPGALEQT
                     QPQEPADAPFVAGPPPSNLIRFLDQRAPRLAAVLSRERLRRGQRHFEHFLERILPDSE
                     WVDWLDSDPVLSGYVFDLFEHSTYFSDQLVRSPELIVELRKLRDAANSRTSYAEIAAH
                     VDELDDLRRSFTREMFRMQCESICLQAPIFQTLERTSDLADAVIQAAYRMALRHTLDS
                     HPPASASYDASGQLMVIALGRLGMREFDLASDADLVFVLSDRDAAELRFWTRVAGRLI
                     DIITAYTGHGVMFAVDTRLRPNGRAGALVQLEGTYKDYFGSHAEAWEGITYMKSRAVA
                     GDVERGTEFLHELQDVDWRRYGQSGRSRKLLAQMRARVEKEQGSENPLKAGRGAYYDI
                     DFALLYLRLKSAGIFFKVLNTPARIDVVEKMGHLERADADFLRDAATFYRAVDHGLRV
                     SSGHAEGSLPKSQSQLDNLTELVCRWTPAHLHDQPLDMELAQIQSRTREFFDRTFA"
     gene            complement(5071..5601)
                     /locus_tag="VLE22_23715"
     CDS             complement(5071..5601)
                     /locus_tag="VLE22_23715"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HSB17477.1"
                     /translation="MWLAARAGYAGRKRAAARPMQGVVLQVNTSPGGIPKRPVPAASL
                     TVFGLEGDACAHPGIHGGSDKAVLIVCAEAVESLIARGYPVFYGALGENLTTRGLDPC
                     FLRAGQRYRVGSEAVIELTKVRRPCATLDVYGPSIKSEIFDSSQRWSLSGFYARVLQP
                     GLIRPGDPMLLLDELA"
     gene            complement(5610..7019)
                     /gene="glnA"
                     /locus_tag="VLE22_23720"
     CDS             complement(5610..7019)
                     /gene="glnA"
                     /locus_tag="VLE22_23720"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HSB17478.1"
                     /translation="MTPKEVLEFAKKNEARQIDLRFTDLPGISHHVSYPISQLEEESF
                     EEGYGFDGSSIRGWAVIHESDMLLLPDPNTAIMDPFTEIPTLVMLADVVDPITRQHYE
                     RDPRWIAKKAEMYLKNSGLADTAYVGAEAEFFIFDSIRFDQNEHCGYYYVDAEEGRWN
                     SGREHDNLGYRPRYKEGYFPTPPTDHYQDLRGEMVETMIRCGLNVECHHHEVATGGQA
                     EIDQRFDTMVKSADNMMLYKYIVRNVANQYGKTVTFMPKPLFGDNGSGMHTHQSLWKN
                     GQPLFAGDGYAGLSQLALWYIGGLLKHARALSAIIAPTTNSYKRLVPGYEAPVNLAYS
                     RRNRSAAVRIPMYSASPKAKRVEFRPPDPSCNPYLSFAAMMMAGLDGILNKIDPGEPL
                     DKDIYDLSPEEMKGVPSMPASLEEALNCLEDDHAFLLKGDVFTEELLESFINYKRKAE
                     SDAVRLRPHPYEFALYYDI"
     gene            7176..8546
                     /gene="xseA"
                     /locus_tag="VLE22_23725"
     CDS             7176..8546
                     /gene="xseA"
                     /locus_tag="VLE22_23725"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HSB17479.1"
                     /translation="MAEQISLDLIPERRIFTVGQLNAAIRSLLDSEFQDVWVAGEISG
                     TKLAASGHYYFTLKEESSQLRCVCFRMTARFLRFKPQDGVAVLARGRVDVYEARGEYQ
                     LLVEALEPQGYGALQLAFEQLKKKLSEEGLFDAGRKRPLPKLPSRIGIVTSPRGAVIR
                     DMLQVLGRRFPGLHIRLYPALVQGEGSVEDVTRAIDYFSGSGWPEVVIVARGGGSIED
                     LWTFNEEAVARAISQCPVPVISAIGHETDFTIADFVADLRAPTPSAAAELVICTKEQL
                     FERVEAAHRKLEHALRYRLADAGRRLHQQGISRASSVVHRMIGRSMQRVDELVYRLRD
                     LMRVKLDRQGRARRELDERLRKLDLRLRLAAWWRRLDPAAAALARHIHLELERRQARL
                     DSLSAQLTQLSPLRILERGYAIVQDEAGRVVKDAEAAPLGVEVGIRLAKGRLRARVTE
                     SVTEKS"
     gene            8714..9007
                     /locus_tag="VLE22_23730"
     CDS             8714..9007
                     /locus_tag="VLE22_23730"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HSB17480.1"
                     /translation="MATYVTLYRWTEQGIKNVKGSPDRISAAKKAVEAQGGKLNNIYV
                     TMGEYDLVAISEWPDEKTAIAFALAMGAQGNVRTTTMRALTQNEFAEIVKKMP"
     gene            9068..9907
                     /locus_tag="VLE22_23735"
     CDS             9068..9907
                     /locus_tag="VLE22_23735"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB17481.1"
                     /translation="MVSRLSVTVLAENWAYEPGLLAEHGLAYFIETDGARILFDTGQG
                     MALAHNAKQLGISLEQLDAIVLSHGHLDHSGGLVEALRAAGRARVFVHPAALGPRYAQ
                     RDKPPRRSAGMPGLCVQELERVAGRVRWTESPTEVIPGIWVTGEIPRRTSFENTSREF
                     YRDAECLDPDPIPDDQALVIDTATGPVILLGCAHAGVVNTLAYVGHLSGQSRIRAVAG
                     GFHLMRAGRERIDDTCDALEQFRVETIAAGHCTGWRAIAQLHRSLGDCVIPCCAGTTL
                     TLG"
     gene            complement(9932..11386)
                     /gene="guaB"
                     /locus_tag="VLE22_23740"
     CDS             complement(9932..11386)
                     /gene="guaB"
                     /locus_tag="VLE22_23740"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HSB17482.1"
                     /translation="MISDGVPEGLTFDDVLLVPARSAVLPAQADTRTWLTRKIALNIP
                     VISSAMDTVTESHMAIALAQQGGLGIIHRNMPVERQAEEVDRVKRSESGMIVDPVTMS
                     PDNRIFEALEVMKRFRISGVPITKEGKLVGILTNRDLRFETRFDLPISEVMTKENLVT
                     VPVGTTLEEAEKILHRHRVEKLLVVDEHYYLKGLITVKDIQKKLKYPNAAKDEQGRLR
                     VGAAIGAGGDFIERAKELIARKVDVLAIDTAHGHSERVMEAIVAVKRALPDTELIAGN
                     VATYDGARELISLGVDGVKVGVGPGSICTTRVVSGSGVPQITAIAEASRASRGTGVPV
                     ISDGGIKFSGDITKAIAAGADAVMIGSLLAGTDESPGETILYQGRTFKSYRGMGSMGA
                     MVQGSSDRYAQDASGKLVPEGIEGRVPYKGPIAEMVYQLVGGLKAGMGYCGCANIAEL
                     QERARFLRVTVAGLRESHVHDVIITKEAPNYRLE"
     gene            complement(11513..12019)
                     /locus_tag="VLE22_23745"
     CDS             complement(11513..12019)
                     /locus_tag="VLE22_23745"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HSB17483.1"
                     /translation="MILQDAVNELAGASHITINKKPISAPELLNEAFHYARPSSFSRG
                     IRIDLNGIAILFISGTASVDENGETAHTGDFRAQCRRTFENITALLSSEGATWKDIVR
                     TTCYLRDIDRDYKEFNEERTAFYAAQGLDPLPASTGIQAKLCRPELLVEIEAIAIFRK
                     ADTPSRER"
     gene            complement(12137..13348)
                     /locus_tag="VLE22_23750"
     CDS             complement(12137..13348)
                     /locus_tag="VLE22_23750"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSB17484.1"
                     /translation="MRGYTAGLILLAAAVLILQPCVGQEPRKPSPDREPYKDARKHHR
                     GYFGAGRDDPEPDLKSLHAVVLGYFGPSDPGHPDGGPAWLGTNLAIDEANASGGYKGL
                     PFRLSPVWAENPWADGAAKLTRVAYLERAWAIIGSIDGASTHLAETIVAKAHLTQIDP
                     GSTDETVNQANVPWMFSCLPGDRPIAAALGGTLLDRAGSSFLLLTATDHDSRALTSEF
                     KSFLSARRVTVKQQLEFPAGTEQTSGLASQAVASGARAVVVLAGARDSARLIRDLRRR
                     DPGLVLLAGPSACRRAFLAEAGTAAEGVIVPVTVETSPLTAAFIEKFQPLYGFEPDYA
                     ATAAYDAASLLIAAIRKSGLNRVRIRDAVEDLSPWRGASGTIDWDPLGRNARPVRLGV
                     IRGGGIAPLGD"
     gene            complement(13345..14817)
                     /locus_tag="VLE22_23755"
     CDS             complement(13345..14817)
                     /locus_tag="VLE22_23755"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSB17485.1"
                     /translation="MRSRLFSGLVLFTLAGSLAAADRKLIDAMWNAELTDPDTLAVLQ
                     RIESAGAYSLPDLNLKKDQNYAQTPQDLEPFRGVKPYKEHFLLQIEYTGAGRGIVEPP
                     DLKSVKIGFIGPIMSTVSVATGGKSHEEALGVKMLEGARLAIEQANARGGYLKRKIPF
                     ELVIANDNGLWGSSGNEIVKMAYKDNVWAIIGTIDGANSHIAIRVALKAELVMMNSGD
                     TDPTFIETNIPWVMRCIGDDRQQSYLLMDYISRKMGLKRIGIIRSSNRYGRFGVREIR
                     DSAFRLGHPVVIEMAYRVGADDFSLQLERLKASNLDAVVHWGDAREGALILNQMRKMG
                     MKQPFFASDRTVSDEFVTLAGKNAEGVICGFPWNPERKDPKFDEFRQDFRARYGQEPE
                     TYAAHAYDGMNMLIWATQLAGLNRAKIRDLLAYRPKPWPGVTGDIPFSAALDDLGEVF
                     LARRENGKWKYHSREDLQIPRGYVPKRDRVTRFAETSQLK"
     gene            complement(14814..15974)
                     /locus_tag="VLE22_23760"
     CDS             complement(14814..15974)
                     /locus_tag="VLE22_23760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17486.1"
                     /translation="MRTRIAILGLLATGTVLASEPASTAPAKLPYVYTKWKQFTVKDG
                     LPNDHIFAVHADGPRVWIGTEDGLAMLDKRTGKIKSWKEKDGLPWKVVTGIDVDPKTG
                     DVWLALFGGGLARFSGGRFDHWHQLNSGLVNDVVYAVTVENDNVWAATTAGASRLNTR
                     TGEWSIFTEKNAPMEEIWNYAVDYRDGKVYLGVWGSGVLEYDIATGRWKDYLDPDGEM
                     EIDLYRDDGIAHVITTGVSYVDKILWVSSYFGASRYDGRKWRVFYNQDSGLPSDFENN
                     MKARSAIEAWYCTDKGLGAIMDFETNTWVAYTRDPKTLRGKALITRDKTVLKEIDLEV
                     GVPHNFIISVDFDGNDVWVGTSKGLGLGIGEGYYPGLGESPAQVAQTKGGAK"
     gene            complement(15984..18848)
                     /locus_tag="VLE22_23765"
     CDS             complement(15984..18848)
                     /locus_tag="VLE22_23765"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HSB17487.1"
                     /translation="MRRMRYLFLCSPVLLVVTLLLTGQGTPKRPVYVGARVCGQCHAG
                     QGMGNQYSLWLHSKHSLAWSGLARPEAKEIATISGLRQDPQESAICLGCHAPAGLAED
                     WEKDDTFQLEDGVQCEACHGPGSEYMDEQVMRNRAAALKAGLKMPTEADCMTCHIEKG
                     SHVAVLKSPQLDAKKAWQEIAHPTPPNAVAGSIPKPPNAFTVASGPKYAGSAACGKCH
                     RGPAMGYQFSEWRRSRHALAFAVLGTPRGYESARKNGVQGDPQKEPKCLKCHTTGQDG
                     PAAFVDSFSLDEGVGCEACHGPGSGYMHEAVMRDKRAAAAAGLKAVSRETCLRCHKDT
                     PEKPFDYAGSLKKIAHPTRPPVKADNPSPKYKTPLNMALRPDGSELYVACEASDSVVV
                     VDPVAKEKVAEIRVGGSPADVTFSPDGRRAFVSNRYDDTVSVIDVASRKVIKVLEAGD
                     EPHGVLTDGQGKLLYVLNTSSEDMTVFDAASLERVKTLSASRGPWSLARSPDSSMILV
                     TNMLSRIPKIREPFVSEITVIETERGVIEDRWVVDGANLMMGVDWHPSGEFALATLNR
                     TKSLVPMTRLLQGWTITNGLAVLWRDGRVDQVLLDEPNMGFADATDIAFTPDGRYALV
                     TSAGTDRVAVVDVNSLISLVRGSSDYEREYVLPNHLGKSSGFIVKYIQTKNSPRGVLV
                     DARHRQAFVANALDDSLTVIDLTRLESAGRLDLGGPKVITKQRLGERLFNNAGITFRK
                     QYACHSCHPDGHIDGVTYDIEADGIGFSPVDNRTLRGILDTAPFKWEGTNPSLSRQCG
                     PRLAVFFTRLSPFTPEQLSAVDNYITTIQRPPNRFHPVGARLTPAQRRGKVIFERTQT
                     NDGREIAVSNRCITCHFPPYYTDRRLHDVGTRHRLDRQGRFDTPHLNNIFDSAPYLHN
                     GIAASLEEIWTVYNPYDKHGVTNDMTKDQLNDLIEYLKTL"
     gene            complement(18845..20494)
                     /locus_tag="VLE22_23770"
     CDS             complement(18845..20494)
                     /locus_tag="VLE22_23770"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB17488.1"
                     /translation="MRFGLALPSVLTAALLAAAAGPELPVFTEISKEAGVQFKHSYGD
                     HHLDNIVEGTGGGACAFDYNNDGFLDLYFVTGTWTRNVSDNEGRDLRGKLFNKLYKNN
                     GNRTFTDVTDQTGVNGKGIFSSACSAADYDNDGYVDLYVLNYGPNILYHNNGDGTFTD
                     VSEKSGLADKHWSLGGVWFDYNNDGYLDVFVANYLLYDDGKFRDFYPAAGYPGPLSYN
                     GEPDALYRNNGDGTFTDMTKEAGLYFPDGRAMGATAADLDNDGDLDIYVTNDAMENYY
                     FENDGKGKFTEKALFKGLAYGEHGQGVSSMGPFIGDVDRNGLLDVYVPDLNYNSLLMQ
                     YEKGFRDQTETSGISVLMAQYAGWAGIMLDYDNDGWLDIFTVHGNAHHEYVQEDTLAR
                     NKGNGTFEDVSRQSGKYFFEKYVGRGATWGDFDNDGDVDLVVVNLNDYAKFLRNDGGN
                     RGNWLTVELKLKFPTGSRDANGARVIVTTGSLRQIEDLTPTRGYLGQGDPRLHFGLGK
                     AGQADSVEIRWPDGNVQKLENVKANQFLKLVRDAKANGSKP"
     gene            complement(20491..22305)
                     /locus_tag="VLE22_23775"
     CDS             complement(20491..22305)
                     /locus_tag="VLE22_23775"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17489.1"
                     /translation="MNRITRFRSTVLALVWGACLAVPSKAGQTLRAHPGDPARPAIVF
                     TQLSAGPSTPSGESTRLVLLRPDGSLHVLSTGFHSAADAEVSFDGKRILFAGKKQASD
                     HWQIFEMQADGTRIRQITREPGDCRSPFYQSQIYIITADSPWHQIAYVGYRSGVPALY
                     SCKLDGSATRRLTYSPLGDRDPHMLPDGRIVFASSQRDRLEPDAIDREALFGINLDGT
                     DFAIFSGDEGAPIKRMPCVTSKRLVVLVESDKPSPDGSGWLASVSLRRNLHSHRVLTR
                     PAEGLFHSPSPLPNGEILVSRRPGNGSGTYGIFRFDPDTGRTVPLHDDPARHDIQARA
                     LVPRPEPDGRSSVVDDREPTARFYCLSAYITDLKNQGWLPPGSMKRLRVVEGVPGEPA
                     GPSLGNGFFGPSAPAPRLSQRILGEINVEDDGSFNIQVPANTPIQFQALDAEGAALRT
                     CKWVWAKNKEVRGCIGCHEDGELTPPNVLAQAVTRPSVPLTLPPERRRTVNFERDIQP
                     LLLRKCASPACHSGAVPPRLDNRVELAKLVVAGRSRVSPLVWSLFGRVTARPWDGPQP
                     ARKEVRPMPPEGSPPLTDDERRTILEWIDLGALFGGLQ"
     gene            22505..23062
                     /gene="efp"
                     /locus_tag="VLE22_23780"
     CDS             22505..23062
                     /gene="efp"
                     /locus_tag="VLE22_23780"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HSB17490.1"
                     /translation="MIQATQLRPGMVIKFNNELFSIFSVFHRTPGNLRGFVQAKMRSL
                     RSGSMTEHRFSSEDRVERAMLEEHEMEYLYDDGEFYYFMNTENYEQMHLTKDILGDAV
                     QYLIPQLKVNIEFYEGKPMSVELPATVDMTVVETEPGLKGATVSNVTKPAKVETGLVV
                     QVPPFINQGEKIRVNTAEGTYQERA"
     gene            complement(23066..24403)
                     /gene="selA"
                     /locus_tag="VLE22_23785"
     CDS             complement(23066..24403)
                     /gene="selA"
                     /locus_tag="VLE22_23785"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HSB17491.1"
                     /translation="MKQHPDGLAFHDLPSVDDVLGRLAGLLSEYPRGLVVEEIRRVLD
                     AMRAEIRTGRSVDPASVDARVASALAELVRPSLRRVINATGVVLHTNLGRAPLPALTL
                     LGGYSNLEYDLAAGKRGNRDVHAAGLLERLLGWPAVAVNNNAAAVYLALNELAAGYEV
                     IVSRGELIEIGDGFRIPDIMARSGAILREVGTTNRTHIGDYRAAITDRTRLLLRVHHS
                     NFQIRGFTARPGLAELAALGRECGIAVYEDLGSGCIVDLRAHGVGEPLVTNSLREGAD
                     LVSFSGDKLLGGPQAGILCGKPEIVGRLRRSPMFRALRLDKLIYQALETTLRHLLLGR
                     ANEIPALRMIAEPAESIRARAERLVRSLDGVPAEVAPGESVIGGGSTPDLSIPTWLIA
                     VAAPDVVALENRLRAGDPPVVARIEKDRLILDLRTVFPEEEAELAAALRRAAA"
     gene            complement(24400..24537)
                     /locus_tag="VLE22_23790"
     CDS             complement(24400..24537)
                     /locus_tag="VLE22_23790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17492.1"
                     /translation="MRSGIDLARTLVAKADEDFVVADDLQVVRRLRAAIHALLPPEAV
                     P"
     gene            complement(24625..24891)
                     /locus_tag="VLE22_23795"
     CDS             complement(24625..24891)
                     /locus_tag="VLE22_23795"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HSB17493.1"
                     /translation="MGRRGICLACVSDMYRALVDDTVLSEIVRHLVAAVDPERIVLFG
                     SRARGDSRPDSDVDLLIVKETDKPKHLRAIPAHRALAGLQVSVE"
     gene            complement(24897..25763)
                     /locus_tag="VLE22_23800"
     CDS             complement(24897..25763)
                     /locus_tag="VLE22_23800"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSB17494.1"
                     /translation="MRKAIFLSLLAAGLLTAQEGKLRIIAFGAHPDDCDGRMGGTAIK
                     LAAMGHMVKFVSVTNGDAGHQREGGGALARRRRAEAQEAGRRFGIAEYVTLDNHDGEL
                     VPSLEVRQQIIRLIRQWNADVVLGPRPNDYHPDHRYTGVLLGDAAFMVTVPNICPDTP
                     ALRKNPVFLYFQDNFQRPNAFRPDIAVDISDVIEKKIDAMDAHVSQYYEWLPWLSGNL
                     DKVPKDPADRRKWLGRERNREVSPAIRKTLEKWYGEEKAKAVRYAEAFEICEYGRRPD
                     DAEIRRLFPMLK"
     gene            complement(25818..26852)
                     /locus_tag="VLE22_23805"
     CDS             complement(25818..26852)
                     /locus_tag="VLE22_23805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSB17495.1"
                     /translation="MSSVPGQFGTRKSPSWLLPAFGYGISLACLIWVYWGFDWETELS
                     RFAAADWRWVTLAVIADVAVYFIQGWRWSLLLSPVARVPFSRSVQAVYVGLFANEVLP
                     LRSGELIRCYVLGKWADTPFSVTLSSAVVERLFDGIWLVIGFFIVSPVLDLPGYLIQG
                     TRILAIVLIAIAILLGVVMFRKHHAHAVVSSSRWSVALSHVVEGLHGMGNSPSFYLAG
                     LVSLVYLAFQIVPVYALMIGYGLDLSFGVAAVTLIVLRLGTILPQAPGNVGAFQFFTV
                     LALSLFAVDKATATGFATMMFVVVTVPLWLAGFIAVAMTGMKIRDLQHHAQSTFRASE
                     ETAAGSEARR"
     gene            26953..29265
                     /locus_tag="VLE22_23810"
     CDS             26953..29265
                     /locus_tag="VLE22_23810"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HSB17496.1"
                     /translation="MQTLRRVAVLGAGTMGSRIAAHFANAGVPVLLLDIVSPVTPDRN
                     ATAVRGIDSALKQRPGAFFTAERTALLSPGNFEDDLGKIGECDWIVEAVVENLDVKRA
                     LWRKVAGVRSNGSIVSTNTSGIPLARIAEGFSEEFRRHFLGTHFFNPPRYLHLVEMIP
                     GAETDPEVLRFTADFCDRRLGKGVVLCKDTPNFIANRIGSFFGATACKIMVEDGYTVE
                     EVDALTGPLIGLPNSATFRLFDIVGLDVWALVFGNVYQLAPDDPWRERFVPPDFLTKM
                     IESGWLGEKSGQGFYKRVGKEKEIHAVDWKTLEYHPARKVRFPAAEEARKIEELTGRL
                     RTLVSSGDRASVFLWKLYRDYLLYAAGRLPEISNRIVEIDRAMRWGYAHNLGPFELWD
                     ALGFENTARRIEREGGPLPPRVEAMLAAGAKSFYRAADESGRPRTEYFDLTASVYREV
                     EERPGVVELAPLKRARGVLKGNAGASLIDLGDGVLCLELHGKSNLLGHDQIEMIHAGI
                     KEAKRNFEAMIVAGVGENFCTGTDLAQILTRAQNRNWSEIDGFIGRLQQANMALKYSA
                     RSVIAAPYGRTSGAGFELALHSARVQASAELYAGFLETAVGLIPAGGGSKEMLLRLGD
                     ARKAFELIGMARVSGSSEEARSLGLLRIEDGVTMNPERLIADAKALALSVARGYEPGA
                     PRNDIPVAGDGGESLMEPGAAGERETEYDLVIARKLALVLGGSPVGRRMLSEQDLLDL
                     EREGFLSLCGDPKTQERIEHMLRTGKPLHN"
     gene            29285..29752
                     /locus_tag="VLE22_23815"
     CDS             29285..29752
                     /locus_tag="VLE22_23815"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB17497.1"
                     /translation="MPNGMLRGSRKKIVVLVGLPGSGKSTYLERLGITPLSSDAVRHL
                     LVDDATDQTIHRQVFAVLRYLVRHRIALGRPLTFVDATHLTPRERRPYLKMGQLYDCV
                     VEALFFDVPLEVCKERNRGRSRIVPEDVMERMAAKLVPPATDEGFSTVTVVHD"
     gene            complement(29726..31036)
                     /locus_tag="VLE22_23820"
     CDS             complement(29726..31036)
                     /locus_tag="VLE22_23820"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB17498.1"
                     /translation="MEHSPVSLSAYWRLVRDNRNFRLVWLAQIVSELGDWLYSIAIYS
                     LLLELTGSAKSVALAVVLQVLPQFFIAPAAGVVNDRMSRKQVMIFADLVRAGIVLFML
                     FVQSPGLVSIIYVLLFAETMMWAFFEPARTSVIPNITHGQEILVANTLSSTTWSVNLA
                     VGSALGGAVAVLFGRNTVFVINALTFLVSASLLRSTRFDEPHVAAARPMKARDLTDFT
                     PLIEGVRYMVRDPRLLATLLVKAGLGFMGTNWVILPILGERVFPISLGRLDPQRAGML
                     GMSMLMGARGVGALLGPLFGGYWAGQKEPRLRTGILYGFLAGTVGYISLGAAPSIWLA
                     FAAVVFAHAGGSVIWVFSTTLLQTHTEDRFRGRVFSADYAFLVVTMSLVSYFAGLFID
                     LGVSVRTLAALTGVFGIAPAIAWGLALRLWNRPGVSSVVHDRDC"
     gene            complement(31038..32027)
                     /gene="trpS"
                     /locus_tag="VLE22_23825"
     CDS             complement(31038..32027)
                     /gene="trpS"
                     /locus_tag="VLE22_23825"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSB17499.1"
                     /translation="MKARVFSGVQPSGNLHIGNYLGALKNWVTIQHDYESIYCIVDMH
                     AVTVYQQPEELRSKILEIAALFLAAGIAPKHSAVMVQSSVPAHAELAWLLTCVTPMGW
                     LSRMTQFKVKAEKQETVGDGLFQYPVLMAADILLYQAAIVPVGDDQAQHLELTRDIAQ
                     RFNSLYGDTFVVPETKLPTVGARVMGLDDPLKKMSKSEPGAGHAVALLDPPGVIRKKI
                     LRATTDSKPAVDYSALGPGVENLLNIFQAFSGWADGQMQSHFSGMRYGDLKKQVAEMV
                     ISHLEPFQKRYHEIVSEPGYLDGVLRDGAARVSPIANSTVELVKRRMGLYVPS"
     gene            32059..33288
                     /locus_tag="VLE22_23830"
     CDS             32059..33288
                     /locus_tag="VLE22_23830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HSB17500.1"
                     /translation="METVETNLARSTRPELTVEKLVYGGAGLGRLEGRAVLVPFVLPG
                     EVVTIEVKRERSGMAEARATAMLLRSPERVEPQCPYFARCGGCSYQHAGYEFQLAQKT
                     AIAREVLRRVGKLEPLGEIAIVSGPPWEYRNRAQFHLDGGRIGFLETGSRRLCPVDRC
                     PISSPRINDALAALRRMIRDRRFPRFLRSVELFTNEREVQLNVLDTDRPLARGFFQWC
                     AEQMPGAGESSLEYTVGGEIYRVGHRSFFQVNRFLIDRLAECALDGVEGMSALDLYAG
                     VGLFSLPLARRCRSVLAVESNASALRDLEFNAERAGLTVAARKATVEQFLETVEAPPD
                     FVLADPPRAGLGKGVVNELLRLRVPVLTIVSCDPATLARDLAPLLAGGYRIDALTLID
                     LFPQTWHIETVTRLRLG"
     gene            complement(33306..34502)
                     /locus_tag="VLE22_23835"
     CDS             complement(33306..34502)
                     /locus_tag="VLE22_23835"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB17501.1"
                     /translation="MPNRRTFLQTLSGLPLIGGLMPATPLLAAAKRDYLKELGVRPII
                     NAAGTFTMFTASLMPPEVVQAWEYGSRHYVRLNELHDAVGKRLAELIGCEAAMVSAGA
                     ASALTLGTAACVTGTNQDFIHRLPEATGMKTEVILQKTHRFGYDHAIRNCGIKLIEIE
                     TLEELERAVNDRTVMMFFLNLAEPAGQIKASEWAELGKKHGIPTFIDCAADVPPVENL
                     SKFTKMGFSLVTFSGGKGLCGPQSAGLLLGRKDLIEAARLNASPYSDAIGRGMKVNKE
                     ELLAMLAAVERYVKMDHKAEWREWERRAKIVSDSIASIPTIQTETKIPEIANQVPHLH
                     IRWDTNVVKISVLDVVKQLREGDPAIEPGPGSREDLIIGVWMMQKGDAQIVARRIRQI
                     LKSAQA"
     gene            complement(34530..37022)
                     /locus_tag="VLE22_23840"
     CDS             complement(34530..37022)
                     /locus_tag="VLE22_23840"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB17502.1"
                     /translation="MTFDHPWLLLFAWLPVAWAALEWRRTPRHAGLVLKAVSLSAILL
                     ALAAPQLTIRSSRMAVAILVDTSASLSPQDLAHASDLATQIEKTRGRHWTRVIPFARS
                     TREATPDELSGSWKLQYTSGDGGRGTDLEAAIREGIAASPAGLVPRLVLISDGKENYG
                     SVARAAWQAQQLRVPVDVYSLSGRPKPELLLESVAVPSLVFTGERFPLDLAIRSPRRT
                     TAAAETAAEGKLLGSSRVTLEPGLNRVRVNTSLTTAGSVELTGTVRAEGAGEVRFAKA
                     LSVRRPKVLLLSQDPRGTEIHLLRTLEAAQFDVQRATEIPRDLDAYQLIVFNNWDLHA
                     VPATAKTAVEQYVQEGGGLLVIGGERNVYIEEKKGQEDPIERALPAKLAPPRTPEGTL
                     VVLIVDKSSSMEGRKMELARLAAIGVIDNLRPIDRVGVLIFDNSFQWAVPIRKAEDRT
                     LIKRLVGGITPDGGTQIAPALAEAYRRALPQNAIYKHIVLLTDGISEEGDSLNLAQQA
                     AANRVTISTVGLGQDVNRAYLEKVATLSKGKSYFLTDPSGLERILLRDVMEHTGSTAI
                     ERPVTPVVMRPAEILEGVGMETAPPLRGYVKFIAKPTADTILTIERKDPLLARWQYGL
                     GRTAVFTSDAKSRWAANWVAWKGFDRFWANLFRDLLPHAQAGETSVEYDSAGDALVVE
                     YRLARQVKEPEKVPDIFVFGPGGFRRPVSVVKVAEGAFRGRVPVGGRQGLFRLRPLEE
                     SRVFPEVGIYLPEAELSEYGSDEALLRQLSQFTGGRFQPDIGSIFQSGGRSVPSTMRL
                     WPGLLATAVLLNLIELVMRKWRGILDALRPTK"
     gene            complement(37019..38959)
                     /locus_tag="VLE22_23845"
     CDS             complement(37019..38959)
                     /locus_tag="VLE22_23845"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB17503.1"
                     /translation="MFLLNLGIGEFLALLGAVSGIVVALYLLDRSRRKLTVATLRFWT
                     PSEQPPEKRTRRRRIQQPLSLILQLLSLLCLLLAIAQLRLGTRDSAGRDHVLILDTSA
                     WMDARSGQGTLMDEARRLALAWVRALPAGDRVMLIRADGLATPATVFESSRAAVEEAI
                     AQSHPGFTALNLDAAFELARQVRHMHSSRTGEVVFAGAGRVTEQQSSAEDRTPPNLRV
                     LPVAAAVENCGLRTIGLRRSHTVADVWEVFVTVRNYGQSSRVVRLALQFGGAPAGLRR
                     IDLPPGAEQNATFELHTRSAGWVEARILGSDDFAADDYAVLELPRQPLVRVAVYSESP
                     AWLRPVLSATPNVEAQFFSPSEYVSNRDADLVILDRFRPPALPSVGSIWIEPPAGASP
                     VRVKTSLRNARVTQWLSGHPLGAGLRVQDLRLESATVFDAAPGDIPIAETEAGPVILA
                     RAGKPRIVVLGFHPLRSSMRYELAAPLLFANLLHWMSPEIFRRWELNAGSVGSVTAVL
                     ESEADATQARVLAEDQIALPFTVQGRNVRFFAGTPGTVRVLAGDRELVYSLTLPEVAV
                     ASWQPPGSVRRGVPSASSRGTLSRDLWQILAILGGLGLLVDWLWFGRGVTMLRGATRA
                     SIPSVTLIRLRDLFGGLARKAS"
     gene            complement(38960..39844)
                     /locus_tag="VLE22_23850"
     CDS             complement(38960..39844)
                     /locus_tag="VLE22_23850"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSB17504.1"
                     /translation="MSQQPLVDRTFLEKLERLTIHWQKSFPGLVGGHNPSRFSGPGQE
                     FLDHRHFHHGDDLRAVNWRAYLRLEKLFLKMFQVEPRTPVRLLIDVSRSMTTGAVSKF
                     DYARKLAAALVYVGLVRLETIAIQPFSDRLFDSFVSAGGRHRFAPAAEFLGQLTPVGV
                     TDSFQAARQFISRYLQRGFLIVISDFLDEDGCERPLQYLADFGHELLLVHLWADEDRV
                     PPWEGELELTDAETGAALQLQFDSAARTRYTSAFDTFAQNLRSLAIHNGGRYVGLSTS
                     LPLEDAIFGPLAQARGVT"
     gene            complement(39854..40843)
                     /locus_tag="VLE22_23855"
     CDS             complement(39854..40843)
                     /locus_tag="VLE22_23855"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSB17505.1"
                     /translation="MGAEAKLRDAVANLDRIRQEIGKVIVGHQDVVDGVLICLLSGGH
                     VLLEGVPGLGKTTLLRTLSQVLHLKYSRIQFTPDLMPADIVGSMVIETDERGGKSLRF
                     QPGPVFANLVLADEINRATPKTQSALLEAMQERTVTSGTTTHEIETPFLVMATQNPIE
                     MEGTYPLPEAQLDRFLMKILVNYPTREQLNQIVERTIHRDDIGLDTVADRAVILSVRA
                     MCREILVAPHVQDFAVDLVLATQPEDNHAHDLAKKYIRYGSSPRGAQGLVESARVLAL
                     MRGRLHVSADDIQDLAPSVLRHRIILNFDAHADGQTTETILREIIRSVAVAAN"
     gene            complement(40919..41365)
                     /locus_tag="VLE22_23860"
     CDS             complement(40919..41365)
                     /locus_tag="VLE22_23860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17506.1"
                     /translation="MISSILIIAFSVVLLVYWFRYTCLLILRTRTPVDYAPVVVTANN
                     LQFPHVQGRIRGAEAVTLDPLRKSLDRDYSLLTYLMKHSAGFQVGGITVEQRMLLLDF
                     KVMQIWYGATRRLAVHQARAALDEMSNILGHLANAIGERAASISRA"
     gene            42217..43137
                     /locus_tag="VLE22_23865"
     CDS             42217..43137
                     /locus_tag="VLE22_23865"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HSB17507.1"
                     /translation="MLPFRLVYHEGYDLNFGEHVFPTQKYRLIRERLLADGLAKPTDF
                     VAPEPAPEQDLVLVHDEGWIQRLRTGTLSFHEILKLEVPYSRQMVNAFWLAAGGTTLA
                     ARLALRDGLGFNIGGGFHHAFHGHGEGFCAVNDVAIAIRRLEREGRIERAMVVDCDVH
                     HGNGTAALFASDRKVFTLSIHQLNNYPAEKPPSDIDINLPDQAGDEEYLDELRKHYVP
                     AVASFQPQLVIYVAGADPYYQDQLGGLALTMAGLKQRDRLVIEMALRHGAAVAVTLAG
                     GYAFRLNDTVAIHVNTVRAGKESMEVTGRA"
     gene            43236..44804
                     /locus_tag="VLE22_23870"
     CDS             43236..44804
                     /locus_tag="VLE22_23870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17508.1"
                     /translation="MTAHAKVAFLLLALAASPSTKGQEPTEPATEKPSPAAKAREMLD
                     AAGEAAGGVRPEIQTAALLHIADNYQLLDKKKAREFAGQAFTAAGSLPQDPVKNRERM
                     QAEVVQVLADIDVPEAVALLRQMQATAGDYDPRWPAIDKIVQVLIQKKDFDAAIELVE
                     GLGSTGFFSYTGARSIFKALPADDPRRTTLFGSAMSAFGVRPHRSFVVFLRECWGDVP
                     KQMAESALNGFLREILDRKSAEEHSQTGVSFATAKGTVSFDSREDSELFDVMHIVRAI
                     NPKRAEELLETRPGLRAWLEQYPKGTESLRAEGWFWTFTVMGDDKQAADRMKQQGRAQ
                     ALTETIASSALNAVREDPDKAISLAQTIPTPARRAEILGSIARSVAGKDPDKARGALD
                     KCITLLDEIKNPNDRVAAWDSVADAAHQINDEKLTSRAIDRALADAAELYKQEEDVEV
                     PNVFLREYWPSTQGYRRAIMRATAILGVDAEPLLLKIVDPELNLFARIAMAQALLGRS
                     QERFPTFAPRKKRR"
     gene            complement(44801..45754)
                     /locus_tag="VLE22_23875"
     CDS             complement(44801..45754)
                     /locus_tag="VLE22_23875"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSB17509.1"
                     /translation="MRIVVIGGTGHIGTFLVPRLVACGHEVVCVSRAQREPYQPHGAW
                     TTVRRVLLDRTSEEAAGTFGARIADLRPDAVIDLICFTEESARQLVETLRGRLQHFLH
                     CGTIWVHGHSVQVPTTEEQPRHPFGEYGCRKAAIEAYLLGEARRSGFPATLLHPGHIV
                     GPGWPPLNPAGHFNPRVFAALARGEDLCLPNLGLETVHHVHADDVAQAFQKALGNWST
                     SVGESFHVVSPAALTLRGYAESVATWFGREANLRFLPWGEWRKTVPENEADATWDHIA
                     HSPNCSIAKAQRLIGYQPRFASLEAVREAVDWLIGNARIEV"
     gene            complement(45763..46923)
                     /locus_tag="VLE22_23880"
     CDS             complement(45763..46923)
                     /locus_tag="VLE22_23880"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HSB17510.1"
                     /translation="MADIVTLNRRSFVGGSLTASVAASAASQGSNPALREKFYGCIAG
                     CHIGSSMGAVVEGWPWQRIEKEHGTAERLLPYEHYRNGWKREPGTTEDGVERQKLMIT
                     AIIEKQDRVNAEDVRAAWLRHIKPESAGMVSEPFEATLLAMAKSGIPARDLGRYCDYA
                     GLNSFARSCHPIGLINAGDITNAVTDVFEVGQLYQTSNSRGLQWAAVTALAIAAATRP
                     DATVDSVLAAILAGDPRQTKETGVAKEIERGLKHTDSCKDFREMRTAFDEVYSGRGTP
                     YAFSSANEVVTKAVCVFRMTKGNVRDAIVAAVNMGRDTDCLAAISAGIAGALTGASSI
                     PAEWVRQVDYATTLNRFTNSQRTLGDHADGLYGAFRARLLRMRAYADEMEKS"
     gene            complement(47017..47391)
                     /locus_tag="VLE22_23885"
     CDS             complement(47017..47391)
                     /locus_tag="VLE22_23885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB17511.1"
                     /translation="MITGLEHTAIASPDHRALAEWYVRNLGFVVNYQSANATFVKAQN
                     GYMIEIIGAEGDRPDTGMKTPGFRHLAVAVSDFDSVYSGLKSRGVAFLTEPVESKGNK
                     VVFFTDPEGNILHLLERQTPLP"
     gene            47452..47823
                     /locus_tag="VLE22_23890"
     CDS             47452..47823
                     /locus_tag="VLE22_23890"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HSB17512.1"
                     /translation="MKRRAFPKEAARRLLRARPSEERDAAMRRVIRSIPKGKVATYAQ
                     VAAAAGYPLYHRQVVQLLRKSGDSLPWQRVLGAGGQIRLRRDMAHEQRMRLEMEGVRF
                     RGKRVAMDEHQHTFRTWELED"
     gene            complement(47839..>48756)
                     /locus_tag="VLE22_23895"
     CDS             complement(47839..>48756)
                     /locus_tag="VLE22_23895"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB17513.1"
                     /translation="ETHTWIQKAADLFGLGTNAIRWITAGSDCRVDTSELRARIEADR
                     AQGGQPFLVVGSAGTVSTGAVDPLPELAKLAREYNLWFHVDGAYGAPAAALPDAGEDL
                     KGLVEADSIALDPHKWLYAPLEAGCTLVRDPQHLLDAFDYSPVYYRFDADAEERPINF
                     YEYGPQNSRGFRALKVWAGLRVAGREGLCRMIADDIDLAKSLYQEVQAHAELEALSQG
                     LSITTFRYVPADLDRHDPAAAAYLNQLNEELVNRIQKGGEAFLSNAVVQGKFALRLCI
                     VNFRTTQSDIRALPEIVVRLGRQTDAELR"
BASE COUNT         9364 a        14653 c        15771 g         8968 t
ORIGIN      
        1 tcaactgcgc gtcgccatgc cgctgggcga gactccgtgg caagtcatcg ccgtcgaggg
       61 taacgcgcgc ctcggcggcg gcgccgccga gagggccgcg aagctgcgca ccggacagca
      121 ccttcgcacg gacggcggca aagtcacgct cgaagcggac gagatcgggc ggatcgaggt
      181 cgcagcgggc tctgaacttc gcgtgacgga atccaccgca gcgaagcaga ccctctcact
      241 gcggcgcgga ctcatccacg cgctgatctg ggcgccgccc cgcagatttg tcgtcgatac
      301 tccgtcggca cgcacgatcg acctcggctg cgagtacacg ctgttcgtcg atgacgatgg
      361 ggacgggctg attcgggtta aaaccggatg ggtcgcgttt cagtttgacg gccgcgattc
      421 tttcatcccg gccggcgccg catgcaggac cacacggcgg ggcggccccg gaactcccta
      481 tttcgaagag gcaagccagg aattgcgccg cgcgcttgcg gcattcgatg cgagcgggaa
      541 cggcggcgac ctgaaacaag tgctcgctga ggcgcggcct cgcgacgggc ttaccctctg
      601 gcacctgctg acacgcgcgg ccgaggccga ccgcggtctc gtcttcgatc gtttcgccac
      661 gttcgttgcg ctgccggccg aggtgacgcg agagggcgtg cttgccaagg accggcggat
      721 gctcgatctt tgctggaacg cgctcaacct cgagaatgct gagtggtggc gcgaatggaa
      781 acgcgcctgg cagccgtgac gttcttccgc aggcgcgtgc ggccccacca ggaagttggg
      841 gcttgcgcac cgctcccttg cggtcgcagc tcggaaactg catgcgcaca ggctggactg
      901 cgaagccgac cacctcagga ctgccggtgt ggcgggcctg gcggtgcact ccacaacaga
      961 aggcacgcac aggcaggaga aaacctcggt acagacacca atttcgcaaa ggcggcgaaa
     1021 ttggagtcag tcccgggttt tctcctgcgc cactcggcag ataagggcct tctcatccca
     1081 tctcggcctg ccggacaaga gctgcagatt atggcgtata gtctggtatg cactgcaatc
     1141 tttgcctagg aggagactgt catgtcctac gttgatcgcc gtcagatctt ccacgccggg
     1201 ttggtcagtc tggccaattt tgtcatggga gagccgctca cttccgaggc gaaggccgag
     1261 acgcggcgag gcatcttcga cgcgcatctc catattcctt ccgataacgg agagaacttc
     1321 cagtggtaca aggtcacaaa gaacatgccg gaattcgtgg cctacctgga tcgttgcgga
     1381 gtgcgccggg gcgtaatctc gtcttcctgg agcaacaagg cgaaaactcc cgacgactac
     1441 cgcaacggca accgcgaggt ggtcaagtac gtggagcagt acaagggccg cttccgggga
     1501 agttgcgtca tcactccttt caaaatcgac gaggcgctgc gtgagatcga aacgtgcgcg
     1561 aagcagttcg gatttgtctg ggtcggcgag tgctgcaact acatgaccgg ctacaagtac
     1621 gaaacgccgg agtggaccga ggtgatgaac ctcgtcaccc ggttaaacct ggtggttcaa
     1681 atccatacga acaccagcga aatgcagtac ctggccgaga aattcccgga tacgaccatc
     1741 gtcttccctc accccggcgg gaatctcaac gatatctccg cccgcatcgc aatcatcgcg
     1801 aagcacaaga acacgcatct cgaactctcc ggatccgggc acgaacgtgt tgggatcgtc
     1861 gagaaggctg tgcaggagat cggcgccgac cgcgtcctct acggttcgga tttcactatt
     1921 aacgagccct caggcgtgat catgcgcgtg aagaatgcgt tcctcacagc gcaggaccgc
     1981 gagaagatcc tctttcgcaa tctggagcgc ctgctggcgg gagccggccc cgggcgtgcg
     2041 cgccagccga agaacacgtg agtgccggcc cgccgagggc tggttctgta agcgtcagag
     2101 ccgcggggtc aggcgaaggt gcggtcgaag aattcgcggg tgcgggactg gatttgggcg
     2161 agttccatat cgaggggctg gtcgtggagg tgggccggag tccagcggca gacgagctcg
     2221 gtgaggttgt cgagctggga ttgcgacttc ggcaggctgc cttcggcgtg tccggacgaa
     2281 acccgtaagc cgtgatcgac ggcgcgatag aacgtggccg cgtcacggag gaagtcggcg
     2341 tcggcccgtt cgaggtggcc catcttctcc accacgtcga tgcgggccgg cgtgttcagc
     2401 accttgaaga agatgccggc gcttttcaac cggagataca gcagcgcaaa gtcgatgtcg
     2461 tagtacgcgc cgcgacccgc cttcagcgga ttttccgaac cctgctcctt ctccacccgg
     2521 gcgcgcatct gcgccagcag cttccgtgag cggccgctct ggccataccg ccgccagtcg
     2581 acgtcctgca gctcgtgcag gaactcggtg ccgcgctcta cgtcgccagc cacggcacgc
     2641 gacttcatgt acgtaatccc ctcccacgct tcggcatgcg agccgaagta atccttatac
     2701 gtgccttcca gttgcaccag cgctccggcg cggccgttcg ggcgcaggcg ggtgtcgacg
     2761 gcgaacatca cgccgtggcc ggtatacgcg gtgatgatgt caatcagacg gccggcgacc
     2821 cgcgtccaga aacgcagctc ggcggcatcg cgatccgaca gcacaaagac caggtcagca
     2881 tcggaggcga ggtcgaactc gcgcatgccg agacggccaa gcgcgatgac catcaactgc
     2941 cccgaggcgt cgtagctggc ggaagccggc gggtgggagt cgagcgtgtg ccggagggcc
     3001 atccgatagg ccgcctggat gaccgcgtcg gcaagatccg acgtgcgttc cagcgtctgg
     3061 aagatcggcg cctgcaggca gatgctctcg cattgcatcc ggaacatctc gcgggtgaac
     3121 gagcgccgga gatcgtcgag ctcgtcgaca tgggcggcga tttcggcgta tgacgtgcgc
     3181 gagttggcgg cgtcccgaag cttgcggagc tcgacgatca gctcagggct acggacaagc
     3241 tgatccgaga agtacgtgct gtgctcaaac agatcgaaga catagccgga aagcaccggg
     3301 tcggagtcca gccagtccac ccactccgag tccgggagga tccgttccag gaagtgctcg
     3361 aaatggcgtt ggccgcggcg cagccgttcg cgcgagagca cggccgccag ccttggggca
     3421 cgctggtcga ggaagcggat cagattactc ggtggcggcc cggcgacgaa tggggcgtcc
     3481 gccggttcct ggggctgcgt ctgttccagc gcgcccggcg agagcgtgta gtagatgggt
     3541 tgttgggcat ggatgacgcg ctcgtagatc tctctgacct cttcgaggtg atggttcagg
     3601 cgcgccagaa gtgcctgggc ggacggttcc tccccaatga gcgcgggcgg catcttgcgg
     3661 gcgagcacct cccgctcgcg gaggtcttcc ggcaacgcgt gggtctggcg gtcttcgtcg
     3721 aactggagcc ggtgttcgag gtaccgcagg aactggtagg ccgaggcgag ccgtgaatac
     3781 tcgatgccgg agagcagacc cttatcgtgg aggcgcgaga gcccgcgaag cgttccgcca
     3841 agacggaccc agggctcgcg gccgccatgg agccgctgga gacactgcac caggaactcg
     3901 acgtcgcgaa tgccacccgg ggcgagcttg atctcaaagc cggagccgcg gcgcgcggcc
     3961 agcttctcgc tgatgcgctc gcgagtggcg gacaccgact cgatggcgcg gaaatcgagc
     4021 gtgctcgagt agatgagggg ttcgttgaac tccagcagct cccggcccaa ccgctggtcg
     4081 ccggccgaga cgcgggccct gatgagcatc tggagctccc agtcacgggc gcggtcgcga
     4141 tagtagctcc tggcgccgtc gagtgagata cagacctcgc cgagacggcc gtccggccgg
     4201 agtcgcaggt cgacgcgata gcacacaccc tcggcggtgt aggtggagag cagatcggta
     4261 agctggttgg cgactttctt gtagaactcc ttgttggtga tccggttcgg gccgtccgtc
     4321 tcaccggccg cgccatagac gaacatgaga tcaatatcag agctgtagtt cagccctttg
     4381 ccgccgagct tgccgagcga aatcaccgcg aagccgcatt cgaggggctc gccgtccccg
     4441 ccataacagc gcggcaggcc gtggcggcgg acaagttccg cgcggatgcg gcggcaggcg
     4501 acctcgagga tggcgtcggc gaggttcgag agctcctcga cggtctcggc gagggtggcg
     4561 aagctgagga cgtcacggag gaggatccga aggatctgtt ttcggcggaa gaccgcgagg
     4621 gtcaggggag acaggacgcc ggagtccccg ccgagggcat tttcgagccg ggtttcgtac
     4681 tcttcggcgg agagcgtccg gtccatgtcg cgggcgccga tggtgtcttc gatccaggaa
     4741 ggctcgcgaa gaatcgcctc ggagaggaag cggctgtagg agaagacggc ggtcagtgac
     4801 gtgaggcccg cgtcggaagc ggtgatccgc gcgaagtcgt ccgggtgctg gaccgccatc
     4861 cgctcgagca ggtgaagaac ctcgtcgggg tccggggagg ctgcaagcag gtgccgcagc
     4921 ttgggtgcag tgtgggccgg cgtcgcctgc gtgacgctgg cgatttgtgc agcggcacga
     4981 accggatcgc ggaagtttac ttgctcgagc agccgctcca tgaagtgcca gtcagattat
     5041 agcctgtgga aggtactgag acagcattgc tcaggcgagt tcgtcgagga ggagcatcgg
     5101 gtcgccaggg cggatcaggc ccggctggag gacgcgggcg tagaagccgc taagggacca
     5161 acgctgagat gagtcgaaaa tctcactctt gatggagggg ccatacacat caagcgtcgc
     5221 gcagggacgc cgaaccttcg tcagctcgat gacggcttcc gagccgacac ggtagcgctg
     5281 gccggcgcgc aggaagcacg ggtcgagacc acgggttgtc aggttctcgc caagggcgcc
     5341 atagaagaca gggtatcccc gagcaatcag gctttcgacg gcttctgcgc agacgatgag
     5401 gacggcctta tccgatccgc cgtggatgcc ggggtgtgcg caggcgtcgc cctcaagacc
     5461 aaacacggtg agggaggccg cgggtaccgg ccgcttgggg atgcctccgg gggaagtatt
     5521 gacctgaagg acgactccct gcatggggcg tgcggcagca cgcttgcggc ccgcgtatcc
     5581 agcgcgggcc gcaagccaca aaccgcctcc tagatgtcgt agtacagcgc gaactcgtag
     5641 ggatgcggac gcaggcgcac ggcgtcagac tcggccttcc gcttgtagtt gatgaaggat
     5701 tcgagcagtt cctcggtgaa gacgtcaccc ttgagcagga aggcatgatc gtcttcgagg
     5761 caattgagag cctcttcgag cgacgcgggc atcgaaggca cgcccttcat ctcttccggc
     5821 gaaagatcgt agatgtcctt gtcgagcggc tcgcccggat cgatcttgtt aagaatgcca
     5881 tcgagtccgg ccatcatcat cgccgcgaac gagaggtacg ggttgcacga cgggtccggc
     5941 ggacggaact cgacgcgctt ggctttcggg ctggccgagt acatcgggat acggaccgcg
     6001 gccgagcggt ttcggcgaga ataagcgagg ttgacgggcg cctcgtagcc ggggaccagg
     6061 cgcttatagc tgttggtggt gggcgcgatg atcgccgaaa gcgcgcgggc gtgcttcaga
     6121 agaccgccga tgtaccagag ggccaactgg ctgaggccgg catacccgtc gccggcgaac
     6181 agcggctgtc cattcttcca gaggctctga tgggtgtgca tgccgctgcc gttgtcgccg
     6241 aacagcggtt tcggcatgaa cgtcaccgtc ttgccgtact ggttggcgac gttccgtacg
     6301 atgtacttgt ataacatcat gttgtcggcc gacttgacca tcgtgtcgaa gcgctgatcg
     6361 atctcggcct gaccgccggt ggccacctcg tggtgatggc actcgacgtt gagcccgcag
     6421 cggatcatgg tttcgaccat ttctccgcgg agatcctgat aatggtcagt cggcggcgtg
     6481 ggaaagtagc cttccttgta ccgcggccgg tatcctaagt tgtcgtgctc gcggcccgag
     6541 ttccagcggc cttcttcggc gtcgacgtag taatacccgc agtgctcgtt ctggtcaaag
     6601 cggatgctgt cgaagatgaa gaactcggcc tccgctccga cataagccgt gtcggcgagg
     6661 ccgctgttct tgagatacat ctcggctttc ttggcgatcc agcgaggatc gcgctcgtag
     6721 tgctggcggg tgatcggatc gaccacgtcg gcgagcatga ccagcgtggg gatctccgtg
     6781 aagggatcca tgatcgcggt gttgggatcc ggcagcagga gcatatcgct ctcgtgaatc
     6841 acggcccagc cgcggatgct cgagccgtcg aagccgtatc cttcttcgaa agattcctct
     6901 tccagttggg agatgggata ggacacgtgg tgcgaaatgc cgggcaggtc cgtgaaccgc
     6961 aggtcaatct gcctggcttc gttcttcttc gcgaattcca gcacttcttt gggcgtcatc
     7021 tacgctttca atcctccatg gacggataca atgacaagtg ggctgccgag ggcctcggcg
     7081 gcgggttcca acgcgccttc aggatagcgt ggggcccgga acccgtcaaa tagaaaaaat
     7141 tgatcggggc tagaagggtg cagagcggac gggagatggc agaacagatc agccttgacc
     7201 tgattccgga gcggcggatc ttcaccgtcg ggcagttgaa cgccgccatc cggtcgctgc
     7261 tcgacagcga gtttcaggac gtctgggtcg cgggcgagat ctccggcacg aaactcgccg
     7321 cgagcggcca ctactatttc accctgaagg aagaaagctc ccagcttcgc tgcgtctgtt
     7381 tccggatgac cgcgcgtttc ctgcggttca agccgcagga cggcgtggcc gtgcttgcgc
     7441 gcgggcgggt cgacgtctat gaggcgcggg gcgagtacca actgctggtg gaggcgctcg
     7501 aaccgcaggg atacggggcg ctccaactag cgttcgagca gctcaagaag aagctctccg
     7561 aggagggact tttcgatgcc ggacggaagc gcccgctgcc gaagctgccg agccgtatcg
     7621 gcatcgtgac gtcaccgcgg ggcgcggtga tacgggacat gcttcaggtc ctcgggcggc
     7681 gtttcccggg actgcacatt cgcctttatc cggcgctggt gcagggggag gggtcggttg
     7741 aggacgtgac gcgggcgatc gactacttca gcggctcggg gtggccggaa gtggtgattg
     7801 tggcacgtgg gggcggctcg attgaggacc tctggacgtt caacgaggaa gcggtggcac
     7861 gcgcgatctc ccagtgcccg gttccggtga tctcggcgat cggccacgag acggacttca
     7921 ccattgccga cttcgtggcc gacctgcggg cgccgacgcc atcggcggcg gcggaactgg
     7981 tgatctgcac gaaagagcag cttttcgagc gcgtggaggc ggcccaccgg aaactcgaac
     8041 atgccctgcg ctaccggctg gcggacgcgg ggcggcgact tcatcaacag ggcatcagcc
     8101 gggcatcctc cgtcgtccac cgcatgatcg gacgctcgat gcagcgggtg gacgaactgg
     8161 tctaccggct ccgggatctc atgcgggtaa agctggaccg ccaaggccgt gcgcggcgcg
     8221 agctcgatga gagactccgg aaactggatc tacggctgcg gctcgcggcg tggtggcgcc
     8281 ggctcgatcc ggcagcggcg gcgctcgcca gacacattca tctcgagctg gagcgccggc
     8341 aggcgaggct cgattccctt tccgcgcagt taacgcaact cagcccttta cgcatcctgg
     8401 agagaggcta cgcgatcgtg caggatgagg cagggcgggt ggtgaaggac gccgaggcgg
     8461 cgccgctggg cgtcgaggtc gggatacggc ttgcgaaggg gaggctgcgc gcgcgagtca
     8521 cggaatcggt taccgagaag agctgaagga tcacgcgatc cggtggtagc gggcgccatc
     8581 gccgcgggcg ttgtctcatc caaggggcgg gcgtataatc aggctatctt tcggcagcca
     8641 tgaggttcgg ccacggctgc ctcacactga ctggccctta tcacacaaga agcaagaaga
     8701 cggaggtatg gctatggcaa cgtacgtcac gctgtacagg tggacggaac aggggatcaa
     8761 gaacgtgaag ggctcgccgg accggatttc agcggccaag aaggctgttg aggcgcaggg
     8821 cggcaagctc aacaatatct acgtcaccat gggcgaatac gacctggtcg cgattagcga
     8881 gtggccggac gagaagaccg caatcgcctt cgcgctggcg atgggcgccc aggggaacgt
     8941 ccggacgacg acgatgaggg cgctcacgca aaatgagttc gcggaaatcg tgaagaagat
     9001 gccgtaaaga cgtgcgccgg catccagacc gtttgcatgc gggcgcgcgg gtgtgaatga
     9061 tagagggatg gtttcgcggc tctcggtgac ggttctggcg gagaactggg cttatgaacc
     9121 ggggttgctg gctgagcacg ggctggcgta tttcattgag acggacgggg ctagaatcct
     9181 gttcgacacg ggccagggga tggccctggc gcataacgcg aagcaactcg ggatctccct
     9241 ggaacagttg gacgcgattg tcctgagcca cggccatctc gaccacagcg gcggactggt
     9301 agaggcgctt cgcgcggcgg ggcgggcgcg tgtatttgtc catccggcgg cgttggggcc
     9361 gaggtatgcg cagcgcgaca agccgccccg ccgcagcgcg ggaatgccag gcctatgtgt
     9421 ccaggaactc gagagggtcg cgggcagagt ccgatggacc gagtcgccga cggaggtgat
     9481 ccccggcatc tgggtcaccg gggagatacc gagacggacc tcctttgaga acacgagcag
     9541 ggagttttat cgggatgctg agtgtctcga tccggatccc attccggacg atcaggcgct
     9601 cgtgatcgac acggccaccg gaccggtcat cctgctgggt tgcgcgcacg ccggtgttgt
     9661 aaacaccctc gcctacgtgg gccacctaag cggccagagc cggatccggg cggtcgccgg
     9721 gggctttcat ctgatgcgcg ccgggcggga acggattgac gatacatgcg atgcgctgga
     9781 gcaatttcgc gtggagacga tcgccgcagg ccactgcacc ggatggcggg cgatcgcaca
     9841 gcttcatcgg agtcttggcg actgtgtgat tccctgctgt gccgggacaa cactgacgct
     9901 cgggtagcgg ctcccgcaag gagcggacga gctactcaag ccggtagttg ggggcttcct
     9961 tggtgatgat gacgtcgtgg acgtgactct ccctgagtcc ggccactgtc acgcgcaaga
    10021 accgtgccct ttcctggagt tcggcgatgt tggcgcagcc gcaatagccc atgccggcct
    10081 tgaggccgcc gaccaactgg tagaccatct ccgcgatggg gcctttgtac ggcactcggc
    10141 cctcgatccc ctcgggcacc agctttcctg acgcgtcctg ggcatagcga tcggaactgc
    10201 cttgcaccat ggcgcccatg gagcccatac cgcgatagct tttgaaggta cggccctggt
    10261 aaaggatcgt ctctcctgga ctctcgtcgg tcccggcgag caggctgccg atcatcaccg
    10321 cgtcagcgcc cgcggcaatg gccttggtaa tatcgcccga gaatttgatg ccgccgtccg
    10381 agatgacggg cacgccggtg ccacggctgg cgcgggaggc ttccgcgatg gcggtgatct
    10441 ggggaacgcc tgagccgctc acgacgcggg tagtgcaaat ggaaccgggt ccgacgccga
    10501 ccttcacgcc gtccacgccg agggagatca gctcgcgcgc gccatcgtaa gtggcgacgt
    10561 tgccggcgat cagctccgta tccggcagcg cccgcttgac cgcgacaatt gcctccataa
    10621 ccctttcgct gtgaccatgg gccgtgtcga tggcgagcac gtcgaccttg cgggcgatga
    10681 gctccttggc gcgctcgatg aagtccccgc cggctccgat ggcagcgccg acacggaggc
    10741 gtccctgctc atccttggcg gcattggggt acttcagctt cttctggatg tccttgacgg
    10801 tgatgaggcc cttgaggtag taatgctcgt cgacgacgag gagtttctcg acgcggtgcc
    10861 gatgcaggat cttttcggct tcttcgagcg tcgtgccgac gggcacggtg acgagattct
    10921 ctttggtcat cacctcggag atcggcaagt cgaagcgggt ctcgaagcgc aaatcgcggt
    10981 tcgtgaggat gccgacgagc ttcccttcct tggtaatcgg cactccggag attcggaagc
    11041 gcttcataac ttcgagcgcc tcgaagatcc gattgtccgg cgacatggtg acgggatcga
    11101 cgatcatgcc gctctcactc cgcttcacgc ggtcgacttc ttccgcctgg cgctcgaccg
    11161 gcatgttccg atggataatc ccgaggcccc cctgctgagc caaggcaatg gccatgtggg
    11221 attcggtcac ggtatccatc gccgagctga tgacggggat gttcagagcg atcttgcgag
    11281 tcagccaggt ccgggtatcc gcctgcgctg gcaacacggc gctgcgggcc gggacaagaa
    11341 gcacatcgtc gaaggtcagg ccttcaggga cgccgtcaga gatcataggt tttcggtgat
    11401 actcaatcat acgagagggc agaggatggg cgcaaattct gatcccatgg cgccgaacgg
    11461 agcctccgac aaaaatgcgc ggggagaact tggggctggg acttgggagc cgtcagcgtt
    11521 ctcgggaagg ggtatccgcc ttgcggaaaa tggcgatggc ctcgatttcg accagcaact
    11581 cgggccggca gagcttcgcc tggattcccg tggaagcggg gagggggtca agtccctgcg
    11641 ccgcatagaa cgccgtgcgt tcctcgttga attccttgta gtcgcggtcg atatcccgga
    11701 ggtaacaggt agtcctgacg atgtccttcc aggtggcgcc ttcgctcgaa agcagggcgg
    11761 tgatgttctc gaacgttcgg cggcattggg cgcgaaaatc gccggtgtgc gcggtctcgc
    11821 cgttctcgtc gacgcttgcg gtgccggaga tgaacagaat cgcgatgccg ttcaagtcga
    11881 tccggatccc gcgcgaaaac gaactgggcc gcgcatagtg gaaggcctcg ttgagaagct
    11941 cgggagcact gatcggcttt ttgtttatgg tgatgtgaga ggcgcctgcc agctcattca
    12001 cggcgtcctg taaaatcatt ccctgacctc caaagtcaca gggctccccc cgggggcgcc
    12061 ctcccataat cgtcgcgaga aaccttctcc taatataagc tgaatcgatc cgaaaaggga
    12121 aacggggccg cgggactcag tccccgagcg gcgcaatccc tccgccgcga attacaccga
    12181 gccggaccgg acgggcgttc cggccgagcg ggtcccagtc gatggtacca gacgcgcctc
    12241 gccagggaga cagatcctcg acagcgtccc ggatgcggac ccggttaagg ccagacttcc
    12301 gaatggctgc aatgaggagg gaagcggcgt cataggcggc cgtggcggcg tagtcgggtt
    12361 cgaaaccgta gagcggctgg aatttctcta tgaatgcggc ggtcaagggg gacgtttcga
    12421 ccgtgacggg aacgatgacg ccctccgcgg ccgtgccggc ctccgccagg aaggcgcgcc
    12481 ggcaggccga gggtccggcg agcagtacca gaccagggtc ccggcggcgg aggtcgcgga
    12541 tgagccgggc gctgtcacgg gcaccggcaa ggacgacaac cgcgcgggct cccgaggcga
    12601 ccgcctggga cgccagcccg gatgtctgct ctgtgccggc cgggaactcc agttgctgtt
    12661 tcaccgtgac gcggcgggcc gagaggaacg acttgaattc ggacgtcaaa gcgcgggagt
    12721 cgtgatcggt ggcggtcaac aggaggaagg aactgccggc gcggtcgagc agggtacctc
    12781 cgagagcggc agcgatggga cggtcgccgg gtagacacga gaacatccaa gggacattgg
    12841 cctggttgac ggtctcgtcg gtgctgccag gatcgatctg cgtgaggtgg gccttggcga
    12901 cgatagtctc ggcgaggtgt gtggacgctc cgtcgatcga gcctataatc gcccatgccc
    12961 gctcgagata agcgacgcgt gtgagcttcg cggctccgtc agcccatgga ttctcagccc
    13021 agaccggaga cagacggaac ggcagtccct tgtaaccgcc ggaggcgttc gcctcgtcaa
    13081 tggcgaggtt tgttccgagc cacgcggggc caccatcggg atgacctgga tccgatggac
    13141 cgaagtaacc gaggacgacg gcgtgcaggc tcttcaggtc aggctcgggg tcgtcacgcc
    13201 cggcgccgaa atagccgcgg tggtgcttgc gggcgtcttt gtacggttcg cgatcgggcg
    13261 acggcttccg cggttcctga ccgacgcacg gctgcaggat caagacggca gccgctaaga
    13321 ggatcaggcc agcggtgtag cctctcattt caattgagag gtctcagcga accgagtgac
    13381 ccggtcacgc ttcggaacat agccgcgagg gatctgaagg tcctcgcggg agtgatactt
    13441 ccacttgcca ttctcgcgtc tggcgaggaa cacctcgccc aagtcatcga gtgcggcgct
    13501 gaacgggata tcgccggtga cgccgggcca cggcttcgga cgataggcga gcagatctcg
    13561 aattttcgcg cggttcaggc ccgccaactg ggtggcccag atgagcatgt tcatgccgtc
    13621 atacgcgtgg gcggcgtagg tttcgggctc ctggccgtag cgcgcgcgga agtcctgccg
    13681 gaactcgtcg aacttcggat ctttccgctc gggattccac gggaaaccgc agatgactcc
    13741 ttcggcattt ttgccggcca gggtcacgaa ttcgtccgaa acggtgcgat cgctggcgaa
    13801 aaagggctgt ttcatgccca ttttgcgcat ctggttcagg atgagagcgc cctcgcgggc
    13861 atcaccccag tggacgacag cgtcaagatt agaggctttg agccgctcca attggaggct
    13921 gaagtcgtcg gcccccacgc ggtaggccat ctcgatgacg accgggtggc cgaggcggaa
    13981 ggcgctgtcg cggatctcac gaacgccgaa gcggccataa cggttactgc tgcgaatgat
    14041 cccgatccgc ttgaggccca tcttgcggct gatatagtcc atcagcaggt aggattgctg
    14101 gcggtcgtcg ccgatgcagc gcatgaccca ggggatgttc gtctcgataa acgtggggtc
    14161 ggtgtcgccc gagttcatca tgacgagctc ggccttcagg gcgacgcgga tggcgatgtg
    14221 gctgttggcg ccatcgatcg tgccaattat cgcccagacg ttatctttgt acgccatctt
    14281 gacgatctcg ttgccggagg agccccagag accgttgtcg ttggcgatga cgagttcgaa
    14341 agggatcttg cgtttcaggt aaccgccacg tgcgttggcc tgctcgattg ccagccgcgc
    14401 gccctcgagc atcttgacgc cgagggcttc ctcgtggctc ttgccgccgg ttgcgaccga
    14461 aacggtggac atgatgggac cgatgaagcc gatcttgacg gacttgagat ccggcggctc
    14521 gacgattccg cgcccggcgc cggtgtactc gatctgaagc agaaagtgtt ctttgtaagg
    14581 cttgacgccg cgaaacggct cgaggtcctg gggcgtctgg gcgtagttct ggtctttctt
    14641 gagattcagg tccggcaacg agtacgctcc ggccgactca atgcgctgga gaacggccag
    14701 cgtgtccggg tcagtaagtt cggcgttcca catcgcgtcg atcaacttac gatcggcggc
    14761 agccagcgat ccagcaaggg tgaacagaac cagcccggaa aacaggcggg atctcatttc
    14821 gcacctcctt tggtctgcgc gacctgcgca gggctctctc ccagtccggg ataataaccc
    14881 tcgccgatgc ccaatccgag gcccttagac gtgccgaccc acacgtcgtt gccatcgaaa
    14941 tcgactgaaa tgatgaagtt gtgcggcacc cccacctcca ggtcgatctc cttgagaacg
    15001 gtcttgtcgc gggtgatgag cgctttcccg cggagcgtct ttggatcgcg ggtataggcc
    15061 acccaggtgt tggtttcgaa atccatgatg gcaccgaggc ccttgtcggt acaataccag
    15121 gcctcaatcg cacttcgcgc tttcatgttg ttttcgaaat cactcggtag tccggaatcc
    15181 tggttgtaga agacccgcca tttccggccg tcgtagcgcg aggcgccaaa atagctcgaa
    15241 acccaaagaa tcttatccac atagctgacg ccggtggtga tgacatgggc gataccgtcg
    15301 tcgcggtaga gatcgatctc catttcgccg tcggggtcga ggtaatcctt ccagcgcccg
    15361 gtggcgatgt cgtattcaag cactccgctg ccccacacgc ccaggtagac cttgccgtcc
    15421 ctatagtcga cagcgtagtt ccagatctct tccatcggtg cgttcttctc cgtaaagatc
    15481 gaccactcac cagtgcgggt gttcaagcgg cttgcgccgg cggtggtggc ggcccagacg
    15541 ttgtcattct cgaccgtgac cgcgtaaacc acatcattga cgaggccgga gttaagctga
    15601 tgccagtgat cgaaacgtcc gccgctgaag cgcgcgagac caccgccgaa tagcgccagc
    15661 cagacatcgc cggttttggg atcgacatcg atgccggtga ctactttcca cgggaggccg
    15721 tctttttcct tccaactttt gatcttgccc gtgcgcttat cgagcatcgc gaggccatct
    15781 tcggtgccga tccagacgcg aggcccgtca gcgtgaacgg cgaagatatg atcgttcggc
    15841 aggccgtctt tgaccgtgaa ctgcttccac ttcgtgtaga cgtaagggag cttcgccggc
    15901 gccgtggagg caggctccga ggccagcacc gtgcccgtgg ccaacagtcc caggattgcg
    15961 attctcgttc gcatgtggcc ctcctacagg gtcttgaggt attcgatcag gtcgtttagc
    16021 tgatccttgg tcatgtcgtt ggtgaccccg tgcttgtcgt aggggttata gacggtccag
    16081 atttcctcca gggaagctgc tatcccgtta tggaggtaag gcgccgaatc gaagatgttg
    16141 tttaggtgcg gcgtgtcgaa acggccctga cggtcgaggc ggtggcgtgt gccgacgtca
    16201 tgcaaccggc ggtccgtgta gtacggcggg aagtggcacg ttatgcaacg attgctgacg
    16261 gcgatttcgc ggccgtcgtt ggtttgcgtg cgctcgaaga tcactttccc acgccgctgc
    16321 gccggcgtta gtcgggcgcc aaccggatgg aaacggttcg ggggcctctg aatggtcgtt
    16381 atatagttat cgaccgccga caactgttcc ggcgtgaagg ggctcaggcg ggtgaagaag
    16441 accgccaggc gcgggccgca ttgccgtgac aggctgggat tcgtaccctc ccacttgaac
    16501 ggagcggtgt cgaggatgcc gcgaagagtc cggttatcga cggggctgaa gccgatgccg
    16561 tcagcctcga tgtcgtacgt gacgccgtcg atgtggccgt ccgggtggca cgagtgacaa
    16621 gcgtactgtt ttcggaatgt aatcccggca ttgttgaaga gccgctcccc caaacgctgc
    16681 ttggtgatga ctttcggccc gccaagatcg aggcgcccgg cgctttccag acgggtcagg
    16741 tcgatcacgg tgagcgaatc gtccagcgcg ttcgcaacga acgcctggcg gtggcgcgcg
    16801 tcgaccagca ctccgcgagg gctgttcttg gtctggatgt acttgacgat gaacccggac
    16861 gacttgccga ggtggtttgg cagcacgtac tcgcgctcgt agtccgatga cccgcgaacg
    16921 aggctgatca gcgagttgac gtccaccaca gccacacgat ccgtgccggc gctggtgacg
    16981 agcgcgtacc ggccgtccgg agtaaacgcg atgtcggtgg cgtcggcgaa gcccatgttc
    17041 ggctcgtcga gcagaacctg gtcgacccga ccgtcccgcc agagaacggc gagaccgttg
    17101 gtaatcgtcc agccctggag aaggcgagtc atcggcacga ggctcttcgt gcggttcagc
    17161 gtggccaagg cgaattcgcc ggacggatgc caatcgacgc ccatcatcaa gttggcaccg
    17221 tcaacaaccc accggtcttc gatcactccc cgttccgtct cgatcaccgt gatttccgag
    17281 acgaacggct cgcggatctt gggaatgcgc gagagcatgt tcgtcacgag aatcatcgag
    17341 gaatcgggcg agcgcgcgag agaccacgga ccgcgactgg cgctcaacgt cttgacgcgt
    17401 tcgagagaag cggcgtcgaa taccgtcatg tcctcggagg acgtgttcag cacatagagc
    17461 agcttcccct ggccgtcggt gagaacacca tgaggctcgt ctccggcttc gagcaccttg
    17521 atcactttcc gggatgcgac atcgatcacc gaaaccgtgt cgtcgtagcg gttgctgacg
    17581 aaggcgcgcc gtccgtccgg agagaacgtg acgtccgcgg gactgccacc gacccggatt
    17641 tcggcgacct tctccttcgc gaccggatcc accacgacaa cagaatccga ggcttcgcag
    17701 gcgacgtaga gttcgctgcc gtcgggccgg agcgccatgt tgagcggggt cttgtacttc
    17761 gggctgggat tatcggcttt gaccggcggg cgagtcgggt gagcgatctt tttcagcgag
    17821 ccggcgtagt cgaacggctt ctcgggagtg tccttgtggc atcgaaggca ggtctcgcgc
    17881 gagacggcct tcaagccggc ggcggccgcg gcgcgtttgt cccgcatgac ggcttcgtgc
    17941 atatagccgc tgccggggcc gtggcaggcc tcacagccaa cgccttcgtc gagagaaaac
    18001 gagtcgacaa acgccgccgg gccgtcctgg cctgtggtgt ggcacttcag gcacttcggt
    18061 tctttctggg gatctccctg aactccgttc tttcgcgccg attcgtagcc gcgcggcgtc
    18121 ccaagcaccg cgaacgccag ggcatgacgg ctgcggcgcc actcgctgaa ctggtagccc
    18181 atcgccgggc ctcgatgaca tttgccgcac gcggcggagc cagcgtattt cggaccggac
    18241 gctactgtga aggcgttcgg cggtttgggt atcgagccgg ctacggcgtt tggcggagtg
    18301 ggatgcgcaa tctcctgcca cgctttcttg gcatctagct ggggagactt gaggacggcg
    18361 acgtgggagc ctttctcgat atggcaggtc atgcagtcgg cttccgtggg catcttcagg
    18421 ccggccttca gtgccgcggc gcgattccgc atgacctgtt cgtccatgta ctcgctgccg
    18481 gggccatggc aggcctcgca ctgaacacca tcttcgagtt ggaaggtgtc gtccttctcc
    18541 cagtcctcgg ccaagccggc gggggcatgg cagcccaggc aaatggccga ttcctgggga
    18601 tcctgccgga ggccgctgat cgtagctatc tctttcgctt ccggccgcgc gagacccgac
    18661 caggcaagac tgtgcttcga gtgcagccac aagctgtatt ggttccccat gccttggccg
    18721 gcgtggcact gaccgcatac tctcgcacca acgtagaccg gccttttcgg tgtgccctgc
    18781 ccggtcaaga ggagcgtcac gaccagaagt accggactgc agaggaacag gtaacgcatg
    18841 cgcctcatgg tttgctcccg ttcgcctttg catcccgaac cagcttcagg aactggttcg
    18901 ccttcacatt ctcgagtttc tgaacgttgc cgtcgggcca gcggatctcg accgaatcgg
    18961 cttgcccggc cttccccaac ccgaagtgga ggcggggatc gccctgacca agatacccac
    19021 gtgtgggtgt gagatcttcg atctggcgga gcgatccggt ggtgacgatc actcgcgcgc
    19081 cattggcgtc gcgcgagccg gtggggaact tgagtttcaa ttcaacggta agccagttgc
    19141 ctcggttgcc accgtcgttg cgcagaaact tggcgtagtc gttcaggttg accacgacca
    19201 gatcgacgtc gccgtcattg tcgaaatcgc cccatgtggc tccgcggccg acgtacttct
    19261 cgaagaagta cttgcccgac tgacgcgaga cgtcctcgaa ggtgccattc cccttgttgc
    19321 gggccaaagt gtcttcctgg acgtactcgt ggtgggcgtt gccgtggacg gtaaagatat
    19381 cgagccagcc gtcgttgtcg tagtcgagca tgatgccggc ccacccggca tactgggcca
    19441 tgaggacaga aatgccggaa gtctcagtct ggtcccggaa gcccttctcg tactgcatca
    19501 acaggctgtt gtagttgagg tcagggacgt aaacatcgag cagtccgttg cggtccacgt
    19561 cgccgatgaa gggacccatg gacgagacgc cctggccatg ctccccataa gcaagaccct
    19621 tgaacaaagc tttctcggtg aacttaccct tcccgtcgtt ctcgaaatag tagttctcca
    19681 tcgcgtcgtt ggtgacgtag atgtcgaggt cgccgtcgtt gtcgaggtcg gcggcggtgg
    19741 cgcccatggc tcgcccgtcg gggaagtata gacccgcctc tttagtcatg tcggtaaacg
    19801 taccgtcccc gttgttgcgg tagagagcat ccggttcgcc gttgtagctc agcggtccgg
    19861 ggtagccggc ggcgggatag aaatcccgga acttgccgtc atcgtagagc agatagttgg
    19921 cgacgaagac atcgagatag ccgtcattgt tgtaatcgaa ccatacaccc ccaaggctcc
    19981 agtgtttgtc cgcaaggccg gatttctcag acacatccgt gaaagtgccg tcgccattgt
    20041 tgtggtaaag gatgttcgga ccgtagttga gcacgtagag gtcgacatag ccatcgttgt
    20101 cgtagtcggc ggcggagcag gcggaactga agatgccctt gccgttaacg cccgtctgat
    20161 cggtgacgtc ggtgaacgtc ctgttgccgt tgttcttgta gagcttgttg aagagcttgc
    20221 cgcgaagatc tcggccctcg ttgtcgctga cgtttcgggt ccacgtgccg gtaacgaaat
    20281 agaggtcgag gaagccgtcg ttgttgtaat cgaaggcaca ggcgccgccg ccggtgccct
    20341 cgacgatgtt atcgagatga tgatcgccgt agctgtgctt gaactggaca ccggcttctt
    20401 tcgagatttc agtgaaaacc ggcagctccg gcccggccgc ggccgcaagg agagcagcgg
    20461 tcagaacaga cgggagggcc aatccgaatc tcattgcaga cctccaaaga gtgctccgag
    20521 gtcgatccat tcgaggatgg ttcttctttc gtcgtccgtc aagggtgggg agccctcggg
    20581 tggcatgggg cgaacctcct tccgggcggg ttgcggtcca tcccacgggc gcgcggtcac
    20641 cctgccgaaa aggctccaga cgagcgggct gacccgggat cggcccgcaa cgaccagctt
    20701 cgccagttcc acgcggttgt cgagccgggg cgggacggcg ccggagtgac acgccggact
    20761 ggcgcacttc cttaggagaa gcggctggat atcgcgttcg aagttgaccg tgcgacgcct
    20821 ctccggcggg agtgtcaagg gcaccgaggg acgggtgacc gcctgggcga gcacgttcgg
    20881 cggagtgagt tcgccgtcct catgacagcc gatgcatccg cgaacctcct tgttcttcgc
    20941 ccagacccac ttgcaggtac ggagcgcggc tccctccgcg tcgagcgcct gaaactgaat
    21001 cggcgtattg gcgggaactt gaatgttgaa ggacccgtcg tcctcgacgt tgatctcgcc
    21061 gagaatgcgc tgcgacaggc gtggcgccgg agcggacggg ccgaagaacc cgtttcccag
    21121 agaagggccg gcgggctcgc ccgggactcc ctcgacgacc cggaggcgtt tcatcgaacc
    21181 gggcggtaac cagccctggt ttttcaagtc ggtgatataa gcgctcaggc aataaaagcg
    21241 cgccgttggc tcgcggtcgt caaccacgga agaacggccg tcgggctcgg gccgcggaac
    21301 cagcgcgcgg gcctggatgt cgtgccgcgc cgggtcgtca tgcagtggga cagtacgccc
    21361 ggtgtcggga tcaaagcgga agatgccgta cgtgccagag ccattccccg gccggcggga
    21421 gacgagaatc tcaccattgg gcagaggcga tggtgagtga aagaggccct ccgccggacg
    21481 ggtgagtacg cggtgggagt ggaggtttcg ccgcaggctg accgaggcaa gccaacccga
    21541 gccgtcgggg gagggtttat cggattccac gagaacgacc agccgtttcg acgtgacgca
    21601 cggcatccgc ttgatgggag cgccttcatc ccccgagaaa atcgcgaagt ccgtgccgtc
    21661 gaggttaatg ccaaacaagg cctcgcggtc gatggcgtcc ggctccagcc gatctcgctg
    21721 gctggaggca aagacgatcc ggccgtcggg aagcatgtgc gggtcccggt caccgagcgg
    21781 gctgtacgtc aggcggcggg tggcggagcc gtccagtttg caggagtaca aggccggcac
    21841 accgctccgg tagccgacat aggcgatctg gtgccaggga ctgtcggcgg tgatgatgta
    21901 gatctgcgac tggtagaagg gactccggca gtcccccggc tctcgggtga tctggcgtat
    21961 gcgggtcccg tccgcttgca tctcgaagat ctgccagtgg tcggaagcct gttttttccc
    22021 ggcgaagagg atgcgtttcc catcgaagga gacctcggca tcggccgcgc tatgaaatcc
    22081 ggtactcaga acgtggaggg agccgtccgg acggagaagg acgaggcggg tagattctcc
    22141 tgacggggta gaagggccgg cggaaagctg ggtgaagacg atggccggac gtgccggatc
    22201 gccgggatgt gcccgcagag tctggccagc cttcgaggga acggcgaggc acgcgcccca
    22261 taccagcgcc agaacagttg acctgaatct ggtgattctg ttcatctcac tcccactgcg
    22321 tccccaatcc gtgccaggac cactagcgag accggcagac aatcggacct ggcactccgt
    22381 tccgcgctgt ccttgggccg attataccgt gagccgggcg gggagaagtc agccggccgc
    22441 gtctgataaa atgttgaatc ggcctgctgt accaacctaa acaaagcagg gagtcttttc
    22501 ttcgatgatt caagccaccc aattgcgtcc gggcatggtg atcaagttca acaatgagct
    22561 gttctcgatc ttcagcgtct tccatcgcac gcccggaaac ttgcggggat tcgtgcaggc
    22621 gaagatgcga agtctgcgaa gcgggtcaat gaccgagcac cgcttctctt cggaagaccg
    22681 cgtcgaacgt gccatgctcg aagaacacga aatggagtac ttgtacgacg acggcgagtt
    22741 ttactacttc atgaacaccg agaactacga gcagatgcac ctgacgaaag acattctcgg
    22801 ggacgccgtc cagtatctga ttccgcagtt gaaggtcaac atcgagttct atgaaggcaa
    22861 gccgatgagc gtcgaactgc cggcgacggt ggatatgacg gtcgtggaga ccgaacctgg
    22921 gctgaaaggc gctacggtct ccaacgtgac caagccggca aaggtcgaga cggggcttgt
    22981 cgtgcaggtg ccaccgttca tcaaccaggg cgagaagatc cgtgtgaaca ctgcggaagg
    23041 aacataccag gaacgggcct gaggcttacg ccgcagcccg gcgaagggcg gcggccagct
    23101 ccgcttcttc ttcggggaac acagtacgaa ggtccagaat cagccggtcc ttctcaatcc
    23161 gagcgactac cggagggtcg ccggctcgca gccgattctc caaagcgacg acatcgggcg
    23221 ccgcgacagc gatcagccaa gtcgggatcg acaggtcggg cgtggaacct ccgccgatca
    23281 ccgattcacc gggcgccacc tccgccggca caccgtccag cgaacgaacg agcctttccg
    23341 cccgcgcacg aatactctcc gcaggttccg cgatcatgcg aagcgcgggg atttcgttcg
    23401 cgcggccaag caggagatgg cgcagcgttg tttcgagagc ctgataaatc agcttgtcca
    23461 ggcgcaacgc gcggaacatc gggctgcggc gcaaccgccc cacgatctcc ggcttcccac
    23521 agaggatgcc tgcctgcgga ccgcccagga gtttgtctcc gctgaaagag acaagatcag
    23581 ccccttcacg caggctgttt gtaacgaggg gctcgccgac accatgagcg cgcagatcca
    23641 ctatgcagcc gctaccgagg tcttcgtaaa ccgcgatccc gcactcgcgc cccagcgcag
    23701 ccagctccgc taaaccgggt ctggcggtaa agcctcggat ctggaaattg ctgtggtgca
    23761 cccggagcag aaggcgggtg cggtcggtga tcgcggcgcg atagtcgccg atatgagtgc
    23821 ggttggtggt gccgacctcg cgaaggatgg cgccggagcg ggccatgatg tccgggatgc
    23881 gaaagccatc gccgatttcg atcagctcac cgcgagaaac gatgacctcg taaccagcgg
    23941 ccagttcatt gagggccagg tagacggcgg cggcattatt gttcacggcg acggccggcc
    24001 agccgagaag ccgctccagc aggccggcgg cgtggacatc gcgattcccg cgtttgccgg
    24061 cggcgagatc gtactcaagg tttgagtagc ctccgaggag cgtcaaagcg ggaagaggcg
    24121 cacggccgag gttcgtgtga agcacgacgc ccgtggcgtt gatcacgcgc cggagcgacg
    24181 ggcgcaccag ttccgccaga gcggacgcca cccttgcgtc caccgaagcc gggtcgacgg
    24241 agcggcccgt gcggatttcc gctcgcatgg catcgagcac gcgccggatc tcctcgacca
    24301 ccagaccccg tggatactct gaaagaagtc ccgccagccg gccgagcacg tcatcgaccg
    24361 aagggaggtc gtggaatgcg agaccgtcgg gatgctgctt cacggcactg cctccggggg
    24421 aagtagcgcg tgtatggcgg cccgaagtct tcgcacgacc tggagatcgt ccgcgacaac
    24481 gaagtcctcg tcagccttcg cgaccaacgt ccgcgcaagg tcaatgccgc ttctcatgaa
    24541 gtacccgccc ctctttcaac gctcgtccga tcaggtgccc aggaacagtg gcccattccg
    24601 agacttccgc gggagttctc caaactattc caccgacacc tggaggcccg ccagcgcccg
    24661 atgagctgga atggccctaa ggtgctttgg tttgtcggtt tctttgacaa tgagtagatc
    24721 gacgtcgctg tccggccgcg aatcaccccg tgcccggctg ccaaaaagca cgatccgctc
    24781 aggatcgaca gcggcgacga ggtgcctcac gatctcgctc aatacggtgt cgtcaacaag
    24841 ggctcggtac atgtcgctca cacaggcaag gcagatgcct cgtcggccca ttcagactac
    24901 ttcagcatgg ggaaaagtcg gcggatttcg gcatcgtcgg gccgacggcc gtattcgcag
    24961 atctcgaagg cctcagcgta ccggacggcc tttgcctttt cctcgccgta ccacttctca
    25021 agcgtcttcc ggatggccgg gctcacctct cgattgcgct cgcgtccgag ccattttcgg
    25081 cggtccgcgg gatccttcgg caccttgtcg agattacccg atagccacgg cagccactcg
    25141 tagtactggg agacgtgcgc atccatggca tcgatcttct tctcgatcac atcggagatg
    25201 tcaacggcga tatccggacg gaacgcattg ggcctctgga agttgtcctg gaagtacagg
    25261 aagacggggt tcttccggag cgccggagta tcgggacaga tattgggcac cgtcaccata
    25321 aaagcggcgt ccccgaggag cactccggtg tagcggtgat cggggtggta gtcgttgggc
    25381 cgcgggccga ggaccacatc ggcgttccac tggcggatta gccggatgat ctgctgccgg
    25441 acttccaggc taggaaccag ctcgccgtcg tggttgtcga gcgtaacgta ctcggcgata
    25501 ccgaaacggc gtccggcttc ctgggcttcg gcgcgccgcc tccgggcgag cgctccaccc
    25561 ccctcgcgtt ggtgcccggc gtcgccgttg gtgacggaga cgaacttcac catgtgcccc
    25621 atggcggcga gtttgattgc cgtgccaccc atccggccat cgcagtcatc cggatgcgcg
    25681 ccgaacgcga tgatccggag tttgccctcc tgcgccgtca aaaggccggc ggccagcaac
    25741 gataaaaaga tcgcttttcg catgtgtcag ctcccgaatt cttccttcgc tggagttctc
    25801 cttgcgggaa ttatatgtca acgccgggcc tcggacccgg cggcggtctc ctccgaggcg
    25861 cgaaacgtgc tctgcgcatg gtgctgaaga tcgcgaatct tcatccccgt cattgctacc
    25921 gcgatgaacc cggcaagcca gagaggcacg gtaacgacca cgaacatcat ggtggcgaag
    25981 ccggtcgcgg tggctttgtc gacggcaaac aggctcagcg ccaacacggt gaaaaactga
    26041 aaggcgccca cgttgccggg cgcttgagga agaatcgtgc ccaatcggag aacgatcaac
    26101 gtgacggccg ccacaccgaa tgagagatcc agcccgtaac cgatcatcag cgcatagacc
    26161 ggcacgatct gaaaggcaag gtagaccaga ctgaccaggc cagccagata gaacgagggc
    26221 gagttgccca taccatgcag gccctcaaca acatgcgaaa gagcaaccga ccagcggctc
    26281 gatgaaacca ccgcgtgcgc atggtgcttc cggaacatta cgaccccgag gagaatcgct
    26341 atggcgataa ggacgatggc gaggattcgc gtaccctgga tgaggtaacc cgggaggtcg
    26401 agaaccgggc tgacgatgaa aaacccgatg accagccaga tgccgtcgaa cagacgctcg
    26461 acgaccgccg aagacaaagt cacggagaag ggtgtatccg cccacttgcc aagcacatag
    26521 caacgaatta gctcgccgct ccggagcggg agcacttcat tggcaaaaag cccgacatac
    26581 accgcctgga cgctccgcga gaacggcacg cgcgcgacgg gactcaggag caggctccat
    26641 cgccagccct gaatgaaata gaccgccacg tcggcgatca cggcaagcgt gacccaacgc
    26701 cagtccgcgg cggcgaagcg tgacaattcg gtttcccagt caaagcccca gtagacccag
    26761 atcaggcatg ccagcgagat cccgtatcca aacgcgggaa gaagccagga agggctcttt
    26821 ctggtgccga actgcccagg aacggaagac attcgcttca ggaaccatca taacgagcgg
    26881 cacgcagtcc cgaggaggcg cgtttctcgc agtgtatgga tttcggtaca ctccgttctg
    26941 ggacgtacct cgatgcaaac actccggcgc gtagccgttc tgggcgcggg cacgatggga
    27001 tcgcgaatcg ccgcacactt tgcgaacgcc ggcgttccgg ttctgctgct ggacattgtt
    27061 tcgcccgtca cgccagatcg gaatgccacc gcggtgcggg ggatcgacag cgcgctgaag
    27121 caacggccgg gtgcgttttt caccgcggag cgaacggctt tgctctctcc gggcaacttt
    27181 gaggacgatc tcgggaagat cggggagtgc gactggatcg tcgaggcggt ggtcgagaac
    27241 ctcgacgtca agcgtgcgct ttggcggaag gtcgccggag tacgttccaa cggatccatc
    27301 gtttcaacca acacgagcgg catcccgctg gcgagaatcg ccgagggatt ctccgaagag
    27361 ttccggcgcc acttcctcgg aactcatttt ttcaacccac cgcgctattt acatttggtc
    27421 gagatgattc cgggcgctga gacggacccc gaggtgctgc gtttcacggc ggacttctgc
    27481 gacaggcggc tcggtaaggg agtagtgctc tgcaaggaca cgccaaattt catcgcgaac
    27541 cgaatcggca gcttcttcgg agcgacggcg tgcaagatca tggtcgagga cggctacacc
    27601 gtcgaagagg tggacgccct taccgggccc ctgatcgggc tcccgaacag tgcgaccttc
    27661 cggctgttcg acatcgtggg gctcgatgtc tgggccttgg tttttggaaa cgtttatcag
    27721 ttggcgcccg acgacccatg gcgggagcgc ttcgttccgc cggacttcct cacgaagatg
    27781 atcgagagcg gttggctggg cgagaagagc ggacaaggat tctacaaacg cgtcggaaag
    27841 gaaaaagaga tccacgcggt cgactggaaa accctcgagt accaccccgc gcggaaggtt
    27901 cgttttccgg ccgccgaaga ggcgagaaag atcgaagagc tgacaggcag gctccggacc
    27961 ctggtcagtt ccggcgaccg ggccagcgtt ttcctatgga agctgtaccg cgactatctg
    28021 ctctacgccg ccggacggct gccggagatc tcgaaccgca tcgtcgagat cgaccgggcg
    28081 atgcgctggg gctacgccca caatctgggg ccgttcgagc tctgggatgc actgggtttc
    28141 gagaacaccg cccggcggat cgagcgcgaa gggggcccgc tcccaccgcg cgtggaagcg
    28201 atgctggcgg ccggcgcgaa gtccttctac cgggcggcgg atgaatcggg aaggccacgc
    28261 acagaatact ttgatctcac ggcaagcgtc taccgggagg tcgaggaacg gcccggcgtg
    28321 gttgaattag ccccgttaaa acgcgctcgg ggcgtgctga aaggaaatgc cggagcatcg
    28381 ctgatcgact tgggcgacgg agtgctctgc ctggaacttc acggcaagtc gaacttgctg
    28441 ggccacgacc agatcgagat gatccatgcg gggatcaaag aggcgaagcg aaatttcgag
    28501 gcgatgatcg tcgccggcgt cggcgagaat ttctgcaccg gtacagacct ggcgcaaatt
    28561 ctgacgcgtg cgcaaaatcg aaactggagc gaaatcgacg gattcatcgg acggctccag
    28621 caggcaaaca tggcgctcaa atactccgcc cggtcggtga tcgccgcgcc gtacgggcgg
    28681 acgtctggcg ccggattcga gcttgcgctg cactcggctc gcgtgcaggc gtccgccgag
    28741 ttgtacgctg ggtttcttga aactgcggtt gggctcattc cggctggggg aggatcgaaa
    28801 gagatgctcc tccggctcgg cgacgcccgg aaggccttcg aattgatcgg catggcacgc
    28861 gtctccggaa gctccgaaga ggcgcgaagc ttgggtctat tgcggatcga ggacggtgtc
    28921 acaatgaacc ccgagcggct gatcgccgac gcgaaagcgc tggctctgtc ggtggctcgt
    28981 ggatacgaac cgggcgcacc gcgaaacgac atacctgtgg ccggagacgg cggagagtcg
    29041 ctgatggagc ccggtgcagc cggcgaacgg gagacggaat acgatctcgt catcgcgcgc
    29101 aagcttgcct tggtcctcgg cggcagtcct gtgggaagac ggatgttatc tgagcaggac
    29161 ctgctcgatc tggaacgaga gggtttcctc agcttgtgcg gcgacccgaa gacgcaagag
    29221 cgaattgagc acatgctaag gacgggaaag ccactacaca attgaaagaa gatcagatta
    29281 ttcgatgccg aacgggatgt tgcggggttc acgaaagaag attgtggtct tggtggggct
    29341 gccaggctcg gggaagtcga cctacctcga acggctcggc atcactccgc tttcttccga
    29401 tgcggttcgc catctgcttg tcgacgacgc aaccgatcaa acgatccacc ggcaggtgtt
    29461 cgctgttctc cgttacctgg tgcgacaccg gatcgccctc ggccggccgc tgacgttcgt
    29521 ggacgcgacg catctcacgc cgcgcgagcg ccgaccctat ctcaagatgg gccagctcta
    29581 tgattgcgtg gtggaggccc tctttttcga cgtgccgttg gaggtgtgca aggaacgaaa
    29641 tcgcggccgc tcgcgtatag ttcccgagga cgttatggag cgaatggcag cgaaactggt
    29701 gccgccagct accgacgaag gcttctcaac agtcacggtc gtgcacgact gaactgacgc
    29761 ccggccggtt ccaaagccgc agagccagac cccacgcaat cgccggtgca attccgaata
    29821 cgcctgttag agcggccaag gttcgcacag agacgccaag gtcgatgaac aaaccggcga
    29881 agtaactcac caacgacatg gtcacgacaa gaaacgcgta atcggcggag aagacccgcc
    29941 cccgaaagcg gtcttcggtg tgcgtctgga gcagagtcgt cgagaatacc cagatcaccg
    30001 agccgcccgc gtgcgcgaac actaccgcgg cgaaggccag ccagatcgat ggcgccgccc
    30061 cgaggctgat gtatccgacc gtgccggcga ggaacccgta aaggatcccg gtacgcagcc
    30121 gaggttcctt ttgtccggcc cagtatcctc cgaacagcgg accgagaagc gcacccacac
    30181 cccgagcacc catcagcatg ctcatcccga gcataccggc gcgttgcgga tcgaggcggc
    30241 cgagagaaat cgggaagacg cgttcaccga ggatcggcag aatgacccag ttggtcccca
    30301 tgaagcccag ccccgctttc acgagcaggg tcgccagaag tcgtggatcg cggaccatgt
    30361 agcgaacccc ttcgatgagc ggcgtgaagt ccgtgagatc ccgtgccttc atcggccggg
    30421 cggcggctac gtgcggctcg tcgaaccggg tggatcggag caggctcgcc gagacgagaa
    30481 acgtgagcgc attgatgacg aataccgtgt tgcggccgaa gagcactgcc accgcgccgc
    30541 caagggcgga gccgacggcg aggttgaccg accaggtggt tgaggacagc gtgttggcga
    30601 cgaggatctc ctgcccgtgc gtgatgttcg ggatcacgga cgtacgggcc ggctcaaaaa
    30661 acgcccacat catcgtctcg gcgaacagca gcacatagat gatcgacacc agacccgggc
    30721 tttggacaaa aagcatgaac agcacgatgc ctgcccgcac gaggtcggca aagatcatca
    30781 cctgcttccg gctcatgcga tcgttgacga cgccggcggc cggggcgata aagaattgcg
    30841 gcagcacctg aagcacgact gccagagcga ccgacttggc actgccggtc aactccagta
    30901 gaaggctgta gatcgcgatg gaatagagcc agtctcccag ttcactgaca atctgggcga
    30961 gccagaccag acggaagttg cggttgtcgc ggaccagccg ccagtaggcc gacagggaaa
    31021 ccgggctgtg ctccatctca agaggggacg tacaagccca tccggcgctt gacgagttcg
    31081 actgtgctgt tggcgatcgg agacacccgc gcggcgccgt cacgcaggac gccgtcaagg
    31141 tagccgggtt cggagacgat ttcgtggtat cgcttctgga agggttccag gtgggagatc
    31201 accatctcgg ccacctgctt cttgaggtct ccgtagcgca ttccggagaa gtgcgactgc
    31261 atctgcccgt ccgcccagcc gctgaatgcc tgaaaaatgt tgagcagatt ctcgactcct
    31321 ggcccgaggg cgctgtagtc caccgcgggc ttggagtcgg tcgtggcccg gaggatcttc
    31381 tttcgaatga cacccggcgg gtcgagcagc gccacagcat ggcccgcgcc cggctcggac
    31441 ttgctcatct tcttgagcgg atcatccagc cccatcacgc gagcaccaac cgtgggcagc
    31501 ttcgtctccg gcacgacgaa cgtatcaccg tacagcgaat tgaagcgctg cgcgatgtcg
    31561 cgcgtcagtt caagatgctg cgcctggtcg tcaccgaccg gcacgatcgc agcctgatag
    31621 aggaggatgt cggccgccat gagaacgggg tactgaaaca acccatcccc gaccgtctcc
    31681 tgtttttcgg ctttgacctt aaactgcgtc atccggctga gccaacccat cggagtgacg
    31741 caggtgagca accacgcaag ctcggcgtgg gcgggcaccg aggactggac cattacggcc
    31801 gagtgtttcg gggcgatgcc ggctgccaga aaaagagccg cgatctccag gatcttcgac
    31861 cgcagctctt ccggttgctg gtacaccgta accgcgtgca tgtcgacgat gcagtagatg
    31921 ctctcgtagt cgtgctggat tgtgacccag tttttcagtg cgcccaaata atttccgatg
    31981 tgaagattgc cggagggttg gacgccggag aagactcgtg ctttcatgct aagtgaggcg
    32041 aaagatttat cgtacgcgat ggaaacggtt gaaacaaatc tcgctcgcag cacgagaccc
    32101 gagctcactg tcgagaagct cgtctatggc ggcgccggtc tcgggcggct cgaagggcgc
    32161 gcggtactcg tccccttcgt gctgccgggt gaagtcgtca caatcgaggt gaagcgggag
    32221 cggtcgggta tggcggaagc gcgggcaacc gctatgctgt tgcggtcgcc agaacgcgtg
    32281 gaaccgcagt gtccctattt cgcccgttgc ggcgggtgca gctaccagca tgcagggtat
    32341 gagttccagt tggctcaaaa aacggccatc gcgcgtgaag ttttgcggcg cgtgggcaaa
    32401 ctggaaccgc ttggggagat tgcgatcgtc tcgggtccgc cgtgggagta tcgaaaccgt
    32461 gcgcagttcc atcttgacgg cgggagaatc ggtttcctcg agacaggatc gcgccggctt
    32521 tgcccggtgg accgttgtcc gatttcatct ccgcggatca atgatgccct ggccgcgttg
    32581 cggcggatga tccgggaccg gcggtttcca cggtttctgc gctctgtcga gttgttcacc
    32641 aacgaacggg aagtccagtt gaacgtactg gacacagacc ggccgctggc ccgcggattt
    32701 ttccagtggt gcgcggaaca gatgcccgga gcgggagaaa gctcactcga gtacaccgtg
    32761 ggcggcgaaa tctacagggt aggtcaccgg tcgttcttcc aggtgaaccg tttcctcatc
    32821 gaccgattgg cggagtgcgc gctggacggc gtggagggaa tgtcggcgct cgatctgtac
    32881 gccggcgtcg gtctgttctc gctaccgctg gcacgacgct gccgaagcgt attggccgtg
    32941 gaatcgaacg cgagcgcgct acgcgacctc gagttcaacg cggaacgagc cggactgacg
    33001 gtggccgcgc ggaaggctac cgtcgagcag tttctcgaga cggtggaagc accgccagac
    33061 ttcgtgctcg ccgatccccc tcgcgcgggc ttgggaaagg gcgtggtcaa cgaattgctg
    33121 cgactaaggg tgccggtctt gacgatcgtt tcctgcgatc cggcgaccct ggcaagggac
    33181 ctcgcgcctc tcctcgccgg aggctaccgc attgacgcac tgacgttgat cgacctcttc
    33241 ccgcagactt ggcacatcga aacagtgacc cggctgcggc tggggtaggt tgcagccctc
    33301 gggccttatg cctgcgctga cttcaggatc tggcggatcc ggcgcgcgac gatttgcgcg
    33361 tcgcccttct gcatcatcca gacgccgatg atcaggtctt cccgcgaacc cggccccggc
    33421 tcgatggccg gatctccctc acgaagctgc ttcacaacgt cgagcaccga gatcttgaca
    33481 acgttcgtat cccagcgaat gtggagatgg ggcacctggt tggcgatctc gggaatcttg
    33541 gtctcggtct gaatcgtcgg gatcgaggcg atactgtctg agacgatctt cgcgcgcctt
    33601 tcccactcgc gccactccgc cttgtgatcc atcttcacgt aacgctcgac ggcggcgagc
    33661 atcgccagca gctcttcctt gttcactttc atgccgcggc cgatcgcatc gctgtacggc
    33721 gatgcgttga ggcgggcggc ctcgatgaga tccttgcggc cgagcaggag gccggcgctc
    33781 tggggcccgc aaaggccctt gccgccggag aaagtcacca aagaaaagcc catcttcgtg
    33841 aacttcgaga gattctcgac ggggggaacg tcggcggcgc aatcgatgaa ggtcgggatg
    33901 ccgtgcttct tgccgagttc ggcccattcg gacgccttga tctggccggc tggctcggca
    33961 aggttcagga aaaacatcat caccgtgcgg tcgttgaccg ctctttcgag ttcctcgagt
    34021 gtttcaatct cgataagttt gatgccgcag ttgcggatcg catggtcgta gccgaaacga
    34081 tgcgtcttct ggaggatcac ctcggtcttc atgccggttg cctcgggaag acggtggatg
    34141 aaatcctggt tcgtgccggt cacacaggcc gcggtaccga gggtcagggc gctggccgcg
    34201 ccggcggaaa ccatggccgc ttcgcagccg atcagttccg cgagccgttt gccgactgcg
    34261 tcgtgcagtt cattcaagcg aacgtaatgt cgtgagccgt attcccacgc ctgcaccacc
    34321 tcaggcggca tcagtgaggc ggtgaacatc gtgaacgtgc cggcggcgtt gatgatggga
    34381 cgtacgccaa gctccttgag gtagtcgcgc ttcgcggcgg caagtagcgg agtcgcgggc
    34441 atgagcccgc cgatcagagg cagcccggac agggtctgca agaaagtcct tcgattcggc
    34501 ataattcctc ccggaattca gtccttactc tacttcgtcg ggcgcagcgc gtccagtatc
    34561 ccgcgccatt tcctcatcac cagttcgatt aggttcagca ggaccgcggt agccagcagg
    34621 ccgggccaga gccgcatggt ggacggaacc gaacggccgc cggattgaaa gatgctcccg
    34681 atgtcgggct ggaaccggcc acctgtaaac tgcgaaagct ggcggaggag cgcttcatcc
    34741 gagccgtatt cgctaagctc agcctccggc agatagatgc cgacttcggg gaacacgcgc
    34801 gattcctcga gcggccgcaa ccgaaacagt ccctgacgcc cgcctaccgg tacccgcccg
    34861 cgaaacgccc cttccgcaac tttgaccacc gacaccggtc ggcggaaacc gccggggccg
    34921 aaaacgaaga tatcggggac tttctccggt tcttttacct gccgcgccag acggtattcc
    34981 accacgagag cgtcgcctgc actgtcgtac tccaccgagg tctccccggc ctgagcgtgg
    35041 ggcaaaaggt cacggaacag gttggcccaa aaacggtcga agcctttcca cgcgacccaa
    35101 ttcgcggccc agcgactctt ggcgtccgac gtgaagacgg cggtccgtcc gagtccgtac
    35161 tgccaccgcg ccaagagagg gtccttgcgc tcgatggtca gtatcgtgtc cgccgtcggt
    35221 ttggcgatga actttacgta gccgcgcagc ggaggcgccg tttccatgcc gacaccttcc
    35281 aggatctcgg ccggccgcat cacgaccggg gttacgggcc tttcgatcgc cgtggaaccc
    35341 gtatgctcca tgacgtcccg caacaaaatc cgttcgagcc ccgatggatc ggtcaggaag
    35401 tacgactttc ctttggacag ggttgcgact ttttccaggt acgcgcggtt gacatcctga
    35461 ccaaggccga cggtggagat cgtcacccgg ttcgccgccg cctgctgagc caggttcagg
    35521 ctgtcgccct cttcggagat gccgtcggta agaagaacga tgtgcttgta gatcgcgttc
    35581 tgcggaagcg cgcggcgata ggcttcggca agggccggtg cgatctgggt gccgccatcc
    35641 ggcgtgatgc cccccacgag acgcttaatc agcgtccggt cctcggcctt ccgaatgggc
    35701 accgcccact gaaacgagtt gtcgaagatg agcacgccga cgcggtcgat agggcgcagg
    35761 ttgtcgatta caccgatggc agccagtcgc gccagttcca tcttcctgcc ttccatcgac
    35821 gacgacttgt cgacgatgag caccacaaga gttccttcgg gagtgcgtgg cggcgcaagc
    35881 ttcgccggca aggcacgctc gattgggtcc tcttgccctt tcttttcctc gatatagacg
    35941 ttccgctcac cgccgattac caacaggccg ccgccttcct gcacgtactg ttcgaccgcc
    36001 gttttcgcag tggcgggaac tgcgtgaagg tcccagttat tgaatacgat cagttggtag
    36061 gcgtcaagat cccgcgggat ctcggtggcg cgctgcacat cgaactgcgc ggcctccagg
    36121 gtgcgcaaca gatggatttc agtgcctcgg ggatcttggg aaagcagcag caccttcggg
    36181 cggcggacgg acagcgcctt ggcgaagcgg acctcgcccg ccccctccgc gcgcaccgtt
    36241 ccggtgagtt cgaccgagcc ggcagtcgtc aggctagtgt tgacgcggac ccggttcaaa
    36301 cccggttcga gcgtcacccg gctcgagccc aggagcttgc cctcggctgc cgtttcggcg
    36361 gccgccgtgg tacggcgcgg cgaccggatc gccaagtcga gtggaaaacg ctcaccggtg
    36421 aacaccaatg acggcacggc gacggactcg agcagcagct caggctttgg acgtcccgaa
    36481 agggagtaca catcaacggg tactctcaac tgctgggcct gccaggcggc gcgcgccacg
    36541 ctaccgtagt tctcctttcc atcggagatg aggaccaacc tcggaaccag cccggcggga
    36601 gacgctgcga tgccctcacg gatggcggcc tcgagatcgg taccccgacc cccgtcaccc
    36661 gacgtgtact ggagtttcca cgaaccactg agctcgtccg gcgttgcctc gcgagtcgac
    36721 cgcgcgaaag gaatgactct ggtccagtgg cgcccacggg tcttttcgat ctgcgtcgcc
    36781 aagtcggagg cgtgcgccag gtcctgcgga gacaggctgg cggacgtgtc cacgaggatc
    36841 gcgacagcca ttctcgaaga acggattgtg agctgtggcg cagccaaggc aagcagaatg
    36901 gcgctcaaag atacggcttt gagaaccaga ccggcgtggc gcggcgtccg gcgccattcg
    36961 agcgcagccc aggccaccgg gagccaggcg aatagcagga gccacgggtg atcgaacgtc
    37021 atgacgcctt cctggccagc ccgccgaaca gatcgcgcaa acggatcagc gtcaccgacg
    37081 ggatgcttgc tctcgtggcc cctcgcagca tggtcacgcc gcggccgaac caaagccagt
    37141 caaccaggag tccgagacct ccgaggattg ccagaatttg ccagagatcg cgagagagcg
    37201 ttccgcgact cgatgcggac ggaactcccc gacggacgct gcccggaggt tgccatgacg
    37261 cgacggcgac ctcaggtaat gtcaacgagt agacgagctc gcgatcaccg gcgagaacac
    37321 gaaccgtacc gggcgtgccg gcaaagaagc gaacgttgcg cccctgcacg gtgaatggga
    37381 gggcaatctg gtcctcggca agcacacggg cctgggttgc gtcggcctca gactcgagga
    37441 cggcggtgac actgcccacg cttccggcat tcaactccca acggcggaag atctccgggg
    37501 acatccaatg gagaaggttc gcaaacagaa ggggtgccgc cagttcgtac cgcatcgagg
    37561 agcgcagggg gtgaaaaccg agaacgacga ttctcggctt gcccgcgcgc gccaagatca
    37621 ccggacccgc ctccgtctcg gcgatcggaa tatcacccgg cgccgcatcg aacaccgtgg
    37681 ctgattccag ccgaaggtcc tgtacgcgca agccagcccc gagagggtga cccgaaagcc
    37741 actgcgtcac ccgggcgttc cgcaaggacg tcttcacgcg gaccggcgaa gcgccagccg
    37801 gcggttcaat ccagatgctg ccgacagatg gaagcgccgg cggccggaat cgatcaagaa
    37861 tgacgagatc agcgtcccga ttcgagacgt actcggacgg gctgaagaat tgcgcttcga
    37921 cattcggggt cgcggaaaga accggacgga gccaggcggg ggactcggag taaaccgcaa
    37981 cgcgaactag cggctgccgg ggcagttcca atacggcata gtcatcggcg gcaaagtcgt
    38041 cagatcccag gatgcgcgcc tcgacccaac cggctgatcg tgtatgcaac tcaaatgtcg
    38101 cgttttgttc ggcccctggt ggcagatcga tccggcgaag gccggcgggt gcaccgccga
    38161 actggagggc caggcgtacc acacgggagg actggccgta gttccggacg gtgacgaaaa
    38221 cctcccagac gtcggcgacc gtgtgagagc gacggagacc gatcgtccgc agcccgcaat
    38281 tttccacggc tgcggcgacg gggaggacac gcaggttcgg cggcgtccgg tcctcggcgc
    38341 tcgactgctg ctcggtgacc cggccagctc cggcgaatac cacctcgcct gttctactgg
    38401 agtgcatgtg ccggacctga cgcgccagtt cgaacgcggc atccagattc aaagccgtga
    38461 agccggggtg ggactgggca atcgcctcct cgaccgcagc ccgactcgac tcaaagacgg
    38521 tcgccggagt ggccaaaccg tcggctcgga tcagcatcac acgatcaccg gccggcagcg
    38581 cccgcaccca ggccagagcg aggcggcgag cctcatccat cagagttccc tggcccgaac
    38641 gcgcgtccat ccacgctgac gtgtccagga tgagcacatg gtcgcggccg gcggagtcgc
    38701 gtgtcccaag ccgcaactga gcgatcgcca acagcaggca gagcaaactt aacagttgca
    38761 gaatgagcga gagaggctgc tggatgcgtc gccgccgtgt ccgcttctcc ggtggctgct
    38821 cggatggcgt ccagaagcgg agcgtggcca cagtgagctt ccgccgggag cgatccagaa
    38881 ggtacagcgc gacaacgatg cccgacacgg cgccgagcag cgcaagaaac tcgccaatgc
    38941 cgaggttgag caagaacatc tatgtcaccc cgcgggcctg cgccaacggt ccgaagatcg
    39001 catcctcgag gggcagcgat gtcgagagcc cgacgtagcg gccaccgttg tggatggcca
    39061 gacttcgaag gttttgcgca aaggtgtcaa acgcactggt gtagcgcgtg cgcgccgcgc
    39121 tgtcgaattg aagctgaagc gccgcgccgg tctcggcatc ggtgagttcg agttcacctt
    39181 cccatggcgg aacccggtcc tcgtcggccc aaaggtgaac gagcagcaac tcgtggccaa
    39241 aatccgccag gtactgcagg ggtctttcgc agccgtcctc gtcgaggaaa tccgaaataa
    39301 cgatgaggaa gccacgctgg aggtaccgcg agatgaactg gcgagcagcc tgaaagctgt
    39361 cggtgacgcc caccggagtg agctggccca gaaactccgc cgccggcgcg aaccggtgac
    39421 gtccccctgc cgatacaaag ctgtcgaaga gccggtcgct gaagggttga atagcgattg
    39481 tctccaaccg cacaaggccc acatacacga gcgcggcggc gagtttccgc gcgtagtcga
    39541 acttcgacac ggcgccggtc gtcatcgagc ggctgacatc gatcaaaagc cgcacgggcg
    39601 tgcgcggttc aacctggaac atcttgagga acagcttctc gagtctcaaa tacgcgcgcc
    39661 agttcacagc tcggagatcg tccccgtgat gaaaatggcg gtggtcgagg aactcctggc
    39721 ctgggccgga gaagcgggac gggttatgac ctcctactaa gcccggaaac gacttctgcc
    39781 agtgtatggt gagacgctct agtttctcga ggaacgttcg gtccaccaga ggctgttgcg
    39841 acatcacctg actctaattg gcggccaccg ccacactgcg gatgatctct cggagaatcg
    39901 tctcggttgt ctggccatcg gcgtgcgcgt cgaagttgag gataatccgg tgacgcagca
    39961 ctgagggagc aaggtcctga atatcgtcag cgctaacgtg gagccgcccg cgcatcaagg
    40021 cgagtacccg ggcggattcc acaagaccct gcgcaccgcg cggcgagctc ccataacgaa
    40081 tgtacttttt ggccaggtcg tgcgcgtggt tgtcttcagg ctgcgtagcg agcacaagat
    40141 ccaccgcgaa gtcctgaacg tgcggcgcaa ccaggatctc gcggcacatc gcgcgaacgc
    40201 tgaggatcac cgcgcggtcc gcgacggtgt cgagaccgat atcgtcacgg tgaattgtac
    40261 gctcgacaat ctggttgagc tgttcgcgcg tcggataatt caccaggatc ttcatcagga
    40321 agcggtcgag ttgggcctcg ggcaacgggt aggtgccttc catctcgatc ggattctgcg
    40381 tcgccatgac gagaaacggc gtctcgatct cgtgcgtggt cgtcccgctc gtcacggtgc
    40441 gctcctgcat ggcctcgagc agcgccgatt gcgtctttgg cgtcgcgcgg ttgatctcat
    40501 cggcgagcac gaggttcgca aataccggac ccggctggaa ccgaagagat ttccctccgc
    40561 gctcgtcggt ttcaatcacc atgctgccaa cgatatccgc gggcatcagg tccggggtga
    40621 actggatcct ggaatacttc aggtgcagca cctgggacag cgttcgtaac aacgtcgtct
    40681 tgcccaagcc cggcacgccc tccagcagta catgtcctcc ggaaagcagg cagatcagca
    40741 caccatccac gacatcctgg tgcccgacga tgacttttcc gatttcctgg cgaattcggt
    40801 ctaggttggc aactgcatct cgcagcttgg cttcggctcc cacatcttca ttctagagca
    40861 ccgccgtgga tgtcgcggcc cgcatcggga atgactcgct tggaaaggaa gaagaagatc
    40921 aggcgcgcga gatactcgcg gcgcgttcgc cgatcgcgtt ggcaagatgg cccaggatgt
    40981 tcgacatctc gtcgagggcc gcacgagcct ggtgcacggc cagccttcgc gtggcgccgt
    41041 accaaatctg catcacttta aagtccagca gcagcatgcg ctgctcgact gtgatccccc
    41101 cgacctggaa gcctgcacta tgcttcatca gataggtcag gagactatag tcgcgatcca
    41161 gcgactttcg cagtggatcg agggtaaccg cttccgctcc gcgaatgcgg ccctgtacat
    41221 gcggaaactg gagattgttc gccgtgacca cgacgggcgc gtaatccacg ggcgtacgag
    41281 ttcgcaggat gagcaggcaa gtataacgga accagtagac gagaagcacg accgagaatg
    41341 caatgatgag aatgctagat atcatcttgc cgtctttcgc tgggtgaact ggctcaaccc
    41401 gaatgtgggg gtggcaccca tgtgcgtact aggccgtcta ttatttcacc caagctgaga
    41461 atatcactgg atgccggtaa gcgcaagggt cttttcgacc ttaggtgtga acttttcgct
    41521 ccagcaagac gacccggttc agcgtttatt agcgggcttc ggaaacagca tgtgtccagg
    41581 agaggctccc ctagcggcgc agtccttagc gtacaaggat gcgtaactta ttgtaatata
    41641 atcgctttcc gaaaatccaa gaacgcgggc agcatgatca atccagtctg gaggaccctc
    41701 gagctcaagg aaaaccccta cgggcgtctc gtccagtgtg gcgattccga ctcggtcaga
    41761 agcctggtac tctgtccggt acttctcata acagaagacg gggacatatc ctatacgtcc
    41821 cagtatctgt cgcagttcga tcgccgttga aaagcggact tcaatttcct cgcgagtttt
    41881 gtacttccct gtggtggcgg gccctttgta cgtgaggagt gccttcgcgc caacctgcct
    41941 cacccgaagg acaatgccgc gattgcggag gtcgccggct ggagtgtcat aaagcacgtt
    42001 gctctcgaaa acgcggcggc gaatgcaccg aaaaccgcat gctcgaagca ggcgtctgcc
    42061 gtgcgcagag tcctgcaacc ccaacttgat ctcagtctcc gtacccgaac cgccccgcga
    42121 catgtttcca gtattatccc agtgtttggc ggtgtggcga catgctgccc acgtgcgctc
    42181 gaacgcgata cacttgccgg ttaggctctc ggtttcatgc ttccgttccg gctcgtatac
    42241 catgaaggct acgacctcaa cttcggcgag cacgtctttc ccacacagaa gtatcgcctg
    42301 atccgcgagc gtctgttagc tgatggactt gcgaaaccga ccgatttcgt tgcccccgaa
    42361 ccggccccgg aacaggacct cgttctcgtc cacgacgagg gctggattca gcgccttcgc
    42421 acaggaacac tgagttttca cgagatcctg aagctggaag ttccctattc ccggcagatg
    42481 gtcaatgcct tttggctggc ggcgggcggc acaacgctgg ccgcgcgcct ggctttgcgc
    42541 gacggacttg gcttcaatat tggaggcggg ttccaccacg cattccatgg gcacggcgaa
    42601 ggattctgtg cagtgaacga cgttgcgatt gccatccgcc ggctggagcg ggaaggccgc
    42661 atcgaacggg cgatggtcgt tgattgcgac gtgcatcacg gaaacggtac cgccgccctt
    42721 ttcgcttccg atcggaaggt cttcaccctg tccatccacc aactcaacaa ttatccggcg
    42781 gagaagccgc cctcggatat cgacattaac cttccggacc aggcgggtga tgaggagtat
    42841 ctggatgaac tccgaaagca ttatgtgccg gccgtcgctt catttcagcc tcaactggtg
    42901 atctacgtcg ccggcgcgga cccttattac caggatcagt tgggagggtt ggccctcacc
    42961 atggctggcc tgaagcaacg ggaccggctg gtcattgaga tggctttgag acacggggcc
    43021 gcggtcgcgg tgacgcttgc gggcggatat gcgttccggt tgaacgacac cgtggcaatt
    43081 cacgtgaata ccgtgagggc cggcaaggaa tcgatggaag tgaccgggcg cgcttagctc
    43141 ttggttgtca gctcgctgtg gccgaccctt gctccctcta atggaggaaa agccagggaa
    43201 ctcggtttca gacgacaacg tctaaaacta atacaatgac ggcacacgcg aaggttgcgt
    43261 tccttctcct ggcactggcc gcgtcgccga gcacaaaggg ccaggagccc acggaacccg
    43321 ctaccgagaa gccaagtcct gctgcgaagg cgcgggaaat gctggatgct gctggtgagg
    43381 cggctggcgg cgttcggccg gagatccaga ccgccgctct tctccacatt gcggacaact
    43441 accagctgct cgacaaaaag aaggctcggg agttcgccgg gcaggccttt accgcggcag
    43501 gttctctccc acaagacccg gtgaagaacc gcgaacgaat gcaggccgag gtcgtccagg
    43561 tccttgccga tattgacgtt ccggaggcgg ttgcattgct ccgccagatg caagcaacgg
    43621 ccggggacta cgatccccgg tggccggcga tcgacaaaat cgtgcaggtc ctgatacaga
    43681 agaaggattt cgacgcggcc atcgagctcg tcgaaggcct tggcagtacg ggcttcttct
    43741 cctacaccgg ggcccgctct atcttcaagg cgctgcctgc cgacgacccc aggcggacga
    43801 ctctgttcgg gtcggccatg tccgcgtttg gcgtccggcc gcaccgaagc ttcgtcgtct
    43861 ttctgcggga gtgctggggc gacgtaccga agcagatggc cgaatccgcg ctgaacggct
    43921 tcttgcgcga aattctcgac agaaaaagtg cagaagagca ttcccagacg ggcgtgagct
    43981 tcgccacggc gaaaggaact gtcagcttcg attcgcgaga ggacagcgaa ctgtttgacg
    44041 tcatgcacat tgtccgcgcc atcaatccga agcgcgcaga ggaactgctc gaaacacgcc
    44101 ccggactccg cgcatggctt gaacagtacc ctaagggaac tgaatccctg cgcgccgaag
    44161 gctggttctg gacgttcact gtgatgggag acgacaagca ggcagccgac cgaatgaagc
    44221 agcaggggcg cgcgcaggcg ctcaccgaga cgattgcttc gtcggcactc aacgccgttc
    44281 gcgaggatcc tgacaaggcc atctcgcttg ctcaaacgat tcccacgccc gcgcggcggg
    44341 ccgagattct tggatcgatt gcccggtccg tcgcagggaa ggaccccgac aaggcgcgag
    44401 gcgccctgga caagtgcatc acgttgctgg acgagatcaa gaacccgaat gaccgggtgg
    44461 ctgcgtggga ctcggtcgcc gacgcggcac accaaatcaa cgatgagaag ctgacctctc
    44521 gtgcgatcga ccgggcgttg gccgacgccg ccgaacttta caagcaggag gaggatgtcg
    44581 aggttccgaa tgtctttctc cgagagtact ggccctcgac ccagggctac cgccgcgcca
    44641 tcatgcgggc gacagcgatt cttggcgtgg acgcggagcc gctgctgctg aagatcgtgg
    44701 acccggagct gaacctcttc gcccggatcg cgatggcgca ggcgctgctc ggacggtctc
    44761 aggaacgatt cccgaccttc gccccgcgga agaaacgtcg ctaaacctcg atccgggcgt
    44821 ttccgatcaa ccagtcgacg gcctcgcgca ccgcttcgag cgaggcgaag cggggctggt
    44881 agccgatcag ccgttgggcc ttcgctatgc tgcaattggg cgagtgggcg atatggtccc
    44941 atgtggcgtc tgcctcgttc tccgggaccg tcttgcgcca ctctccccaa ggtaggaatc
    45001 gcagattcgc ctcgcggccg aaccacgtcg caacggactc ggcgtaacct cgcaacgtta
    45061 gcgcagccgg ggagaccacg tggaagcttt cgcccacgga agtgctccag ttgcccagcg
    45121 ctttctggaa cgcttgggcg acgtcatccg catggacgtg gtgcaccgtc tccagtccga
    45181 ggttcggcag gcataagtcc tcaccccggg cgagagcggc gaaaacccga gggttgaagt
    45241 gtccagccgg gttcaatggc ggccatcccg gaccgacgat atgtcccgga tgcagcagag
    45301 tggccgggaa accgctccta cgggcttcac ccagcagata ggcttcgatc gcggctttcc
    45361 ggcaaccgta ttcgccgaac gggtggcgcg gctgctcttc cgtggtgggc acttgcacac
    45421 tgtgcccgtg tacccatatc gttccacaat gcaggaaatg ctggagccgt cccctcagtg
    45481 tctcgacgag ttgccgcgcg ctctcctcgg tgaagcagat cagatcgatc accgcatccg
    45541 gccgcaagtc cgcgatgcgc gcgccgaatg tcccagcggc ttcctcagag gtccggtcga
    45601 ggagaactcg cctaaccgtg gtccaggcgc cgtgcggctg gtacggctca cgctgggcgc
    45661 ggctcacgca aacgacctca tgaccgcatg ccacgagccg cggtacgagg aaagttccga
    45721 tatgacccgt cccgccaatg actacaattc gcatggtgtc ctttagcttt tctccatttc
    45781 gtccgcgtat gcccgcatgc gaaggaggcg agcgcggaag gcgccgtaca gtccgtccgc
    45841 atgatcgccg agcgttcgct gagaattggt gaaccggttc aacgtagtgg catagtcaac
    45901 ctgacggacc cattccgccg ggatggaaga cgctcctgtg agcgcgccag caattccggc
    45961 ggagatcgca gcgaggcagt ccgtgtcgcg tcccatgttc acggcggcga cgatggcatc
    46021 ccgcacattg ccttttgtca tgcgaaacac gcagacggcc ttggtcacga cctcgttcgc
    46081 cgacgaaaaa gcgtaggggg ttcctcgccc gctgtagact tcatcgaacg ccgttcgcat
    46141 ctcgcggaaa tccttgcagg agtccgtgtg cttaaggccc cgctcgattt ctttagcgac
    46201 tccggtttct ttcgtctgtc gcggatcgcc tgcgaggatg gcggctagga cactgtccac
    46261 ggtcgcgtcg ggcctagtgg cggccgcaat cgcaagcgcg gtaaccgcag cccactgaag
    46321 cccccggctg ttgctggtct ggtagagctg gccaacctcg aagacgtctg tcaccgcatt
    46381 cgtgatgtcc cccgcgttga tgaggccgat cgggtggcac gaccgggcga aggagttcag
    46441 gccggcatag tcgcaatacc tgccgaggtc gcgcgcggga atgcctgact tcgccatcgc
    46501 caacagtgtg gcctcgaagg gttcggaaac cattccggct gactcgggct tgatatggcg
    46561 gagccacgcg gcacggacgt cttcggcgtt gacgcggtcc tgtttctcga tgatcgccgt
    46621 gatcatgagt ttctgccgct cgacgccgtc ctcggtggtg ccgggttccc gtttccagcc
    46681 gttgcggtag tgctcgtacg gcagaagccg ctcggccgtc ccgtgctcct tctcaatcct
    46741 ctgccacggc catccttcga ccacagcacc catcgaggaa ccaatgtggc aaccggcgat
    46801 gcagccgtaa aacttctccc taagcgccgg attcgagcct tgcgatgcag cactggcggc
    46861 gaccgatgcc gtcaggctgc cgcctacaaa cgatctcctg ttcaacgtga cgatatctgc
    46921 catgaagatt gctcctaacg attcacgccc gtgatccggt tcgacaactg ccaagtctac
    46981 gccacggttt ggcagaccgc gcttgcaggc gagcggctac ggcagcggtg tttgccgctc
    47041 gagaaggtgg aggatgttgc cttcggggtc ggtgaagaag accactttgt tccccttgct
    47101 ctccacgggc tcggtgagga aggcgacgcc tcgggacttc aggccggagt agaccgagtc
    47161 gaagtcgctc acggcgacgg cgaggtggcg gaatcctggg gtcttcatgc cggtgtccgg
    47221 acggtcgccc tcggcgccga taatctcaat catgtagccg ttttgcgctt tgacaaacgt
    47281 ggcgttggct gactggtagt tgacgacgaa gccgaggttc cggacgtacc actcagcgag
    47341 agcacgatga tctggagacg ctatagcagt gtgttcgagg ccggtaatca tggtctcaga
    47401 atagcagggg aaccgggcgc gggacgggcg tcgtatgctg atgaacagca gatgaaacgg
    47461 agggcttttc cgaaggaggc tgcacgacgc ctgctgcggg cgcgtccgtc cgaggagcgc
    47521 gacgcggcga tgcggcgagt gatacggtct atcccaaaag gaaaggtggc gacctacgcc
    47581 caagtggcgg ctgccgctgg atatccgctc tatcaccggc aggtggtgca gttgctccgg
    47641 aaatcaggtg attcgctgcc gtggcagcgc gtgctcggcg ccggaggcca aatcagactg
    47701 aggcgcgata tggctcacga gcagcgcatg cgactggaga tggaaggcgt ccgcttccga
    47761 gggaagcgcg tcgccatgga tgagcaccag cacaccttcc ggacgtggga actcgaggac
    47821 tgactcccgc gccatgcatc aacgcagttc ggcgtcggtc tgccgtccga gccggacaac
    47881 gatctccgga agcgcccgga tatcggactg cgttgtgcgg aagttcacga tgcagagacg
    47941 aagagcgaac ttgccctgga ctacggcgtt ggagaggaaa gcctcgccgc ccttttgaat
    48001 acgattcacg agttcctcgt tcaactggtt caggtacgcc gccgcggctg ggtcgtgccg
    48061 gtcgagatcc gccgggacgt atcggaaggt ggtgatgctg agtccctgtg acaacgcctc
    48121 gagttccgcg tgagcctgga cctcctggta cagcgacttc gccagatcaa tatcgtccgc
    48181 gatcatccgg cacagtcctt cgcggccggc cacgcgcagc ccggcccaca ctttcagcgc
    48241 tcgaaagccg cgtgagttct gagggccgta ctcgtagaag ttgatcggcc gttcctccgc
    48301 atccgcgtca aaacggtagt agaccggcga gtaatcgaag gcgtccaaca ggtgctgggg
    48361 gtctcgcacg agggtacagc cggcctcgag cggcgcgtag agccacttgt ggggatcgag
    48421 cgcgatcgag tcggcctcga cgagcccttt gagatcctcg ccggcatcgg gtagggccgc
    48481 ggcgggtgcg ccgtacgctc cgtccacatg aaaccacagg ttgtattccc gcgccagttt
    48541 ggccagttcc ggcaacgggt ccacggctcc ggtgctcacg gtgccggccg agccgacgac
    48601 aaggaacggc tgccctcctt gcgcgcggtc tgcttcgatg cgggcgcgaa gttcggaggt
    48661 gtcgacccgg cagtctgagc cggcagtaat ccaccgaatc gcattcgtgc cgagcccgaa
    48721 cagatcggcg gccttttgga tccaggtgtg ggtttc
//
LOCUS       DATIFQ010000473         4106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2457715, whole genome shotgun sequence.
ACCESSION   DATIFQ010000473 DATIFQ010000000
VERSION     DATIFQ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2457715"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VLE22_23900"
     CDS             <1..361
                     /locus_tag="VLE22_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17514.1"
                     /translation="AFDAWAPFLQGLSGQAMVDAVKTVGPWIAWTTVADYVKHPRFKY
                     DAQLVNYVKQTSELVRAVPPGSGVVDYPAFFAGLKAAGYSGPVAYEMCAVLDGGGSVE
                     NLDRTARQFLTWLAQFN"
     gene            complement(488..2473)
                     /locus_tag="VLE22_23905"
     CDS             complement(488..2473)
                     /locus_tag="VLE22_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17515.1"
                     /translation="MKHTKWLLGILVLGTFPVLGQKTTAESLFREALMKERAEGRLRE
                     AIFRYERIIADFANDKQFAARAMYQLSQIYGQLRDPRARDMLTRLSRTGIAPYAARAQ
                     AMLAEQVPAAVPGPFPAVKLPLDYEVGSPDGRFVVYHKDEWKSGVLYLKELATGVERV
                     LADRWVMHPAWSPDSTRLAYCFWSDDEKIKEIRIVQVSNGETKNLGVNGWPISWTDSG
                     EIFYYLPNYAADGADYFLLAVTGGTPRKVHTGTFLAAVTPDGARLIASKSNKLFVIDL
                     ATGHSQAITAFGGSEGEAMISPDGRLVAFRANPDGRWALYVAPLDRGLPVKNPLKVKD
                     IDVGDAPASGRPWRKWWTREGTLTFPLGRSEANIFRVEMDPKSGRAVDAPLRLTQDAA
                     SNTNPSISPDGKRVAYWYRHRSRGGLAIMESSGANERPLFEQFLWAQAPYWRSPEQIM
                     FLNTKPGDGKKRAVYSLDVNSGALTPVAEPEGLYWYYVPARSEILHVVGDIPKPATVL
                     KAVSLADGKQRVVATIENLEDMAVSPDGKRIAYMAGRRGQSANEFIYEIALMSINGEP
                     LGALLSGQPEWISLNAWSPDGKYLLYTEKAGPRVLNVETRESWPLHEETSDSNWRGGS
                     WSPDGTFILLVKAMQTEERVAWEGVTADAVMRLLEAK"
     gene            complement(2575..3267)
                     /locus_tag="VLE22_23910"
     CDS             complement(2575..3267)
                     /locus_tag="VLE22_23910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB17516.1"
                     /translation="MAKILIVEDDPATLLGLGENLAYEGHAIAAVSQGDEVLDKLAAA
                     KPDLLILDVMLPGISGFEVCRRVRKEDRRLPILMLTARSDEVDKVMGLDLGADDYLTK
                     PFSLSELLARVRALLRRAQTSEEPPLPERLEFGDVRLDFTRYIATKRGEPVHLTAKEF
                     ALIRYMAAHAEEALRRDVLLDQVWGEDVFPTNRTVDTHILSLRNKLEPDASRPRYLLT
                     VHGVGYKFAGGS"
     gene            complement(3245..>4106)
                     /locus_tag="VLE22_23915"
     CDS             complement(3245..>4106)
                     /locus_tag="VLE22_23915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB17517.1"
                     /translation="GLELSLRPRESGPAIERRRQNLFAGALAAAVLLVLLLGWFAFRD
                     VSREVRLASLRSDFVSSVTHELRTPLTSIRLLAETLRLKRTRDPATGDQMLGAIVDES
                     ERLTRLVDNVLSFSRIEKDARAYHQKPIELAGAVNDAIRRFQHVLKRGEFHLVQESDD
                     QTLRVLADDEALSQTLLNLLDNAVKYSGPSREIQLGVYRRGAEAEIRVTDHGIGVPAA
                     EQRHIFEKFYRVPGAAGETTGAGLGLALVKHFAEAHGGRVAVESEPGAGSSFSLWLPL
                     MENDGQDPDR"
BASE COUNT          749 a         1338 c         1216 g          803 t
ORIGIN      
        1 agcctttgac gcatgggcgc cgttcctgca aggtctctcc gggcaggcga tggtcgacgc
       61 tgtgaagact gtcggcccgt ggatcgcctg gaccaccgtg gccgactatg tcaaacaccc
      121 gcgtttcaag tacgacgccc aactcgtcaa ctacgtgaag cagacttcgg agcttgtgcg
      181 tgccgttcca cccggcagcg gcgtcgtcga ctatccggcc tttttcgcgg gactcaaggc
      241 tgccgggtac agcgggccag tcgcctacga gatgtgcgcc gtgctcgatg gcggcggctc
      301 ggtcgagaac ctggatcgca ccgcccgcca gttcctgacg tggctggcac agttcaactg
      361 aagaacctcc cagccgcgcc gaccgccaca ggttgaagcc tctgtgcaaa gccgccgctc
      421 aagcggcggc gcaggcctgc cgcctggacc tgatgaagca tccgaggtga aaacgaatct
      481 cctcgcgtca cttcgcttcg agcaagcgca tcactgcgtc ggcggtgacg ccttcccagg
      541 ccacccgctc ctcggtttgc atcgccttga ccagcaggat gaaggttccg tccggcgacc
      601 aactccctcc tcgccaattg ctgtccgagg tctcttcatg gaggggccag ctttccctgg
      661 tttcgacgtt caacacgcgt ggcccggctt tttccgtgta aagcaggtat ttgccgtccg
      721 gggaccatgc attcaggctg atccattccg gttgcccgga tagcagtgcc cctaaaggtt
      781 ccccgttgat ggacatcagg gcaatctcgt aaatgaactc gttggcgctc tgccctcgac
      841 ggccggccat gtaggcaatc cgcttaccgt ccggtgaaac cgccatgtcc tccaggtttt
      901 caatggtcgc cacgactcgt tgcttgccgt ccgccaacga aaccgccttc agcaccgttg
      961 ctggctttgg aatatccccc acaacgtgca gaatttcgct ccgggccggt acatagtacc
     1021 agtagaggcc ctcaggttca gcgaccggcg tcaaggcgcc gctgttgaca tccagactat
     1081 agaccgcgcg cttctttccg tcccccggtt tcgtattgag gaacatgatc tgttcggggg
     1141 atcgccagta gggtgcctgg gcccacaaga actgctcaaa caggggtcgt tcattggccc
     1201 cgctgctttc catgatggca agcccgcccc tgctccggtg tctataccaa taagctacgc
     1261 gcttaccatc gggagagatc gaagggttag tgttggaagc ggcgtcctgg gtcagccgca
     1321 gaggcgcatc taccgcccgt cctgacttgg gatccatttc gacccgaaaa atgtttgcct
     1381 ctgaccgccc cagggggaac gtaagagttc cctcgcgcgt ccaccacttc cgccatggcc
     1441 gaccggaggc tggtgcgtca ccgacgtcaa tatcctttac ttttagggga ttcttgaccg
     1501 gcagccccct gtctagcgga gcgacataga gtgcccagcg gccgtcagga ttcgctcgga
     1561 aggccaccag ccggccgtcc ggagaaatca tggcctcacc ttctgagccg ccaaacgctg
     1621 tgatcgcctg ggaatggccg gtagcgaggt cgatcacgaa cagcttattg gacttcgatg
     1681 cgatcagcct cgccccatca ggggtgaccg ctgcaagaaa ggtcccggtg tgaaccttac
     1741 gtggagttcc gcccgtcacc gccagcagga aataatccgc gccgtcagcg gcatagttgg
     1801 gtagatagta gaagatctcc ccgctgtccg tccaggagat cggccaccca ttcaccccta
     1861 agttctttgt ttcaccgttg gaaacctgga cgatcctgat ctctttgatc ttctcgtcgt
     1921 cactccagaa gcagtaggca agccgggtgc tgtccgggga ccacgccggg tgcatgaccc
     1981 agcggtcggc cagcacgcgt tccaccccgg ttgcgagttc cttcagatac aacacacccg
     2041 acttccattc atccttgtga tagacgacga atctgccgtc cggagaacca acctcgtagt
     2101 cgaggggcaa ctttactgcc ggaaacggcc ccggaacggc ggcgggaact tgctccgcca
     2161 gcatggcctg cgcgcgagcc gcatacggag caatgcctgt gcggctgagc cgcgtgagca
     2221 tgtccctggc gcgtggatcg cggagctggc cgtagatctg cgacaactgg tacattgccc
     2281 gggcggcgaa ctgcttgtcg ttcgcgaaat cggcgatgat gcgttcgtag cggaagatcg
     2341 cttcgcgcag gcgtccctcg gcgcgttcct tcatcagggc ctcgcgaaac agcgattcgg
     2401 cggtggtctt ctgtccgaga accgggaagg tccccaggac cagaattccg agtagccact
     2461 ttgtgtgctt catgactgcc tcctgaaccc gagcatgccc gcgtttgcct gcccggttgt
     2521 cagagccgcc gctgaatcct gcgaaaagac cgcgaaatct ccgtcagaga attgtcaact
     2581 gccgccggcg aacttgtatc ccacgccgtg cacggtgagc agataccgcg gacgcgatgc
     2641 gtccggctcc agcttgttgc gaagggacag aatgtgcgta tccacagtcc ggttcgtcgg
     2701 aaaaacgtcc tcgccccaaa cctggtcgag caggacgtcc cgccgcaggg cctcctccgc
     2761 gtgcgccgcc atgtatcgga tgagagcgaa ctccttggcc gtcaggtgaa ctggctcgcc
     2821 gcgcttggtt gcgatataac gcgtgaagtc cagccgtacg tcgccgaatt ccagcctctc
     2881 cggcagcggc ggctcttcgc tggtttgcgc tctacgcaac agcgcgcgca cgcgcgccag
     2941 aagttcggag aggctgaagg gcttggtcag gtagtcgtcg gcgcccaggt cgagtcccat
     3001 caccttgtcc acttcgtcgc tgcgcgcggt gagcatcagg atcggcagcc gccggtcttc
     3061 tttccggacc ctccggcaga cctcgaagcc gctgatccca ggcagcatga cgtcgaggat
     3121 gagcagatcc ggcttggccg ccgccagttt gtcgagcacc tcgtcgccct gcgaaacagc
     3181 cgcgatggcg tggccctcat acgccaggtt ctcccctagg cccaggagag tcgcggggtc
     3241 gtcctcaacg atcaggatct tggccatcgt tctccatcag gggcagccag agagagaagg
     3301 agcttcctgc tcctggctcg ctctccaccg ccacgcgtcc tccgtgggct tcggcaaaat
     3361 gctttaccag ggccaatccg aggccggcgc ccgtcgtctc cccggccgcg ccgggcaccc
     3421 tgtagaattt ctcgaaaatg tgtcgctgct cggcggccgg gacgccaatg ccgtggtccg
     3481 taactcggat ctcggcttcc gctcctcgtc gataaacccc gagttgaatt tcccgggacg
     3541 gaccagagta cttaaccgca ttgtcgagca ggttcagcag cgtctggctc agcgcctcgt
     3601 cgtccgcgag gacccgcagt gtctgatcat cgctctcctg gaccaggtgg aattccccgc
     3661 gcttcaagac atgctgaaac cggcggatgg cgtcgttgac ggcccctgcc agttcaatcg
     3721 gcttctggtg ataggcacgc gcgtccttct caatccgcga gaagctgagg acgttatcca
     3781 ccagccgagt aagccgctcc gactcgtcga cgatagcgcc cagcatctga tctcccgtag
     3841 cgggatctcg cgtccgcttc aggcgcaggg tttcggcaag aaggcggatc gatgtgagcg
     3901 gcgtcctgag ttcgtgtgtc acgctcgaaa cgaaatcgga gcggagcgaa gccagcctga
     3961 cttcgcgaga gacgtcccgg aatgcgaacc agcccagcag caggacgagc agcaccgccg
     4021 cggccagcgc tcccgcgaac aggttctgcc gccggcgctc gatggcggga ccggactcgc
     4081 gaggccggag tgacagctca agacca
//
LOCUS       DATIFQ010000474        19623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2266131, whole genome shotgun sequence.
ACCESSION   DATIFQ010000474 DATIFQ010000000
VERSION     DATIFQ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 19623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19623
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266131"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..877)
                     /locus_tag="VLE22_23920"
     CDS             complement(155..877)
                     /locus_tag="VLE22_23920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB17518.1"
                     /translation="MTTLLLKERLKKIFVGGPAAPERPAASPRGPIVSSRSVRATESG
                     TSYSRHSHGLEQFFSHIQSDQNLWILDLAGASQANVGFITSLGHRLYSVDFLRTLDTT
                     FDATGDDPLAGQFDARRIGRFLDENLDFEDRFFDGAMVWDCLEFLAPPLLKETIDRLH
                     RITKPGANLLAFFHAEEKAVSIPSYNYRIGDAGTLLLTPRGPRRAAQHFNNRAIEKMF
                     HQFRSVKFFLARDHLREVIITR"
     gene            complement(889..1443)
                     /locus_tag="VLE22_23925"
     CDS             complement(889..1443)
                     /locus_tag="VLE22_23925"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSB17519.1"
                     /translation="MWKRREEDHSQRQATPPTSAELAREGIPMSTIAARNTEPEGARG
                     GALIGKSVVMKGQVYSREDLYIDGEVEGTVELQEHRLTIGPNGKVQAGIKAREVVVIG
                     TVHGNIEAADKIDIRKDAKLVGDIRTSRIVIEDGAYFKGSIDISRTEVSKPAAPAPKP
                     QGAAVTTGTPQQQTLATGSANPKP"
     gene            1563..2945
                     /locus_tag="VLE22_23930"
     CDS             1563..2945
                     /locus_tag="VLE22_23930"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HSB17520.1"
                     /translation="MKPTRRSWAEPFKIKVVEPVRLTTPEQRLRAIQEAGYNTFLLRS
                     EDVYIDLLTDSGTSAMSDRQWAGMMLGDEAYAGSRNFYHLEEMVQKYYGYKYLIPTHQ
                     GRGAEHIVSQVLIKPGDVIPGNMYFTTTRLHQELAGGRFEDVIIAEAHDPASEHPFKG
                     DIDLDKLAALIREHGREHIPYVSVAATVNMAGGQPISLANLQAVRELTARHGIRIILD
                     ATRVAENAYFIQQREPGQQGRSIASIVMQICSLTDGCTMSAKKDALVNIGGFLGLNDR
                     DVFEEARNLVVVYEGLHTYGGMAGRDMEAVAVGIEESLDDDHIRARVGQVLYLGGKMR
                     EWGIPIVLPIGGHGVFIDARSFLPHLPQEQYPAQALASAIYLDSGVRTMERGIVSAGR
                     DKETGQENKPRLELVRLTIPRRVYTQAHMDVVAESVLSVFEGRAAVRGLRMVYEPRYL
                     RFFQARFEPL"
     gene            complement(2965..4128)
                     /gene="ftsZ"
                     /locus_tag="VLE22_23935"
     CDS             complement(2965..4128)
                     /gene="ftsZ"
                     /locus_tag="VLE22_23935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSB17521.1"
                     /translation="MTLDTETEGLKFEIDQELVTGTRFKVIGVGGGGSNAVARMMSEG
                     LEGVEFHVINTDNQALEAALVPNKLAIGAKLTHGLGAGSNPAIGREAALEDTKRIVDI
                     LEGADMVFVTAGLGCGTGTGATPVVCSLAKELGALTVAVVTLPFSFEGPQRMRQAMRG
                     LEELSATVDTVIAIHNDKLLSLAPRGTSFRDAFRVTDDVVRQAVQGISDIITKTGLIN
                     RDFSDIKATMQGMGYALMGTATAKGENAAVEAARLAINSPLMQEGGIAGAGSVLVNIS
                     GSDKLGLHEVNEACSLIREAARREDVQINFGVVLDELLADAVKVTVIATGFERGAAAA
                     VTQKPAEPEFPAAMAEPEPEPDAAEPPVPEPPAIGDDLDTPAYLRRDRLHFDR"
     gene            complement(4180..5361)
                     /gene="ftsA"
                     /locus_tag="VLE22_23940"
     CDS             complement(4180..5361)
                     /gene="ftsA"
                     /locus_tag="VLE22_23940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HSB17522.1"
                     /translation="MAVGLDAGSKRTRCLIGLAENSHLRLVGCGEADSAGWSKGRIAD
                     PAALAGSVALAVAEAEKQAGVSAQAAVVGAGGIAVQGLNNRGLYEMGRPRQIEDVDLR
                     YAVERASSVQLPADRMVLQMFPQDFSVDGRADFRNPRGALGSRLESFVHVITISTQEH
                     HALVGAVNRAHLAVEETVYEPVAAAYAAVLPDERHEGVAVVDIGAHSTDLVVYYGDAL
                     LHATGLPISGDHFTRDVSFGFCVTHEEAERIKEECGCAILGLTADNSIIEIPSPEGRP
                     AREAARRELNMILEARAEELFLFVRRELARIGMEQALADGIVLTGGTAQLTGLCDMAE
                     RVLNCQARNGLPVGILDWPEHIDTPAWTTAAGLMMYSARLKHRTEADRKNGGFLGRLF
                     H"
     gene            complement(5369..6202)
                     /locus_tag="VLE22_23945"
     CDS             complement(5369..6202)
                     /locus_tag="VLE22_23945"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HSB17523.1"
                     /translation="MARKQNAESVPRSRNWGAVFRHVTVAAGILALAVLGLYVYYRVD
                     QFLATDPRFRLKDTAESGQDSGIRIEGVAHASRARVLAMFAADTGRSVYLVPLRERRL
                     QLLGIDWVGEASVSRLWPDRLIVRIKERTPVAFVQLPAGERGAVHRAALIDADGVILE
                     QPERATFELPVLSGIRRSQSRAVRAARVQSAMRLVQDIGGLAAEVSEIDVSDTDNLKV
                     VQQIGGEAVVLWLGDRNFLSRLQNFRGHYEEIRRRLAQAKAFDLRLDDRITAMGAGSG
                     S"
     gene            complement(6204..7583)
                     /gene="murC"
                     /locus_tag="VLE22_23950"
     CDS             complement(6204..7583)
                     /gene="murC"
                     /locus_tag="VLE22_23950"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HSB17524.1"
                     /translation="MFFRPQHVHFIGIGGIGMSGIAEVLLNLGYQISGSDAKLSPITA
                     RLAGLGARIAEGHAAENIGGAKAVVVSSAIDQRNPELEEARRLQIPIIQRGEMLAELM
                     RLKYGIAVAGSHGKTTTTSMTAALLSHAGLDPTVVVGGRVGTMGGANARVGRSDFLVV
                     ESDESDRSFLKLAPILAIVTNIDREHVDCYATIEDTLAAFVEFVNKVPFYGAAILCLD
                     DENVQRILPSVSRRVVTYGTSAQADLTAGEIDCGPASSDFTLRYRGADLGRFHLPAAG
                     MHNVLDATAAVAVGLELEVKPDTMRAALGEFGGVDRRFQLRGRERGVSVIDDYGHHPT
                     EIRATLAAARLGGYRKVHVIFQPHRYTRTLHLMDDFARAFHQADTVYVLDIYAASEKP
                     IEGVTAEALVERIRAFGHRGAEYAGKIDRAVSSVFAAAGDGDLVLTLGAGNVWQVGEA
                     LLELLRGRN"
     gene            complement(7586..8713)
                     /gene="murG"
                     /locus_tag="VLE22_23955"
     CDS             complement(7586..8713)
                     /gene="murG"
                     /locus_tag="VLE22_23955"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HSB17525.1"
                     /translation="MSEGLTFVMAGGGTGGHVIPALAVARELRKRGHQAVFVGTRRGM
                     EAKLAPAEGFPIEWIDIGGLKRVGAGQTVRTLLQLPRSAARAAAVLRARKAAAVFSMG
                     GYVAGPVMLAAWWRRVPIVLMEPNAIPGMTSRWMGRFVERALLNFPEAAAGFPVGRTE
                     LTGLPVRGEFFAVPPKPRESRLTVLITGGSRGSRRLNQAARESWRFFREASFPVRLLH
                     QTGAEDHAELERGFHESGLDGEVTPFIEDMPAAFECADLLVCRSGAGAVAELAAAGKP
                     AILVPFPFAADQHQLRNAEAIERAGAARLVPDAELTGERLFRETAQMAAEEGLLERMG
                     QAARGLARHGAAERAADLLEELGAACRKPLTRHPESRNNTI"
     gene            complement(8710..9864)
                     /locus_tag="VLE22_23960"
     CDS             complement(8710..9864)
                     /locus_tag="VLE22_23960"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HSB17526.1"
                     /translation="MAQRVQTDWILFFTILAMVAFGLLMLYSASSVVAEVRYGSSGYF
                     FLRQLGWALGAMILMMLLKRRDYRIFNSPWWAFASVGVVLASLLLVYFVDPRAHRWLR
                     IGSMGIQPSEFAKPALIVFLAYFVTRRESAINNKGTLMPAAMMVGLIAGGVVVADLGT
                     ALVLTATAAAIFYVAGLERRYFLTAAGIALVCVLLAIVSKPYRMVRVFAYVDPEYKLL
                     ETRLVKMIDPEGKFKQVLQRSAASRDPDYHVRQSLIAVGSGGPAGLGPMQGNQKLFYL
                     PEAHTDFIYAVIGEELGILGCLFVLAGFVIILWRGLRLYLRAPDHFGRYLALGVTAMI
                     VVQAFFNVSVVLGMVPPKGIPLPMISYGGSSLLSTLVSLGMLQSVSEHSG"
     gene            complement(9894..11231)
                     /gene="murD"
                     /locus_tag="VLE22_23965"
     CDS             complement(9894..11231)
                     /gene="murD"
                     /locus_tag="VLE22_23965"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HSB17527.1"
                     /translation="MKATDFSGARIVVVGMVRSGQALVQLLARTGARVTATDVKPLEE
                     LPGAARILESAGAGFVRQSPEAFRDADWIVVSPGVPADLDLLEEARGHGVRVIGEVEL
                     AGYFLQGKIIGITGSNGKTTTTALTGHILRECGIPVQVGGNIGSAVAGMVDTSRPDQW
                     NVLELSSFQLETTERFRAHIAVALNVTPDHLDRHHTFENYAAAKGRLFRTQQPDDFAV
                     LNADDPVCSGYAGGTAGRTHWFSLKHPVEPGLWMAGGWLYAEGEPFLEATGIPLRGSH
                     NVENVLAAATAARLAGAQMSAIAGAVRTFPGVEHRLEFVRTWRGIDFYNDSKATNVDA
                     TLKAIDAFAGPLWVILGGKDKGSDYRPLGGPLSAKAKAVLLVGAATPIIAEHLHGAVP
                     LAECGTIATAVSRAAAKASAGDTVLLAPACASFDQFENFEHRGRVFKELVQQL"
     gene            complement(11228..12361)
                     /gene="mraY"
                     /locus_tag="VLE22_23970"
     CDS             complement(11228..12361)
                     /gene="mraY"
                     /locus_tag="VLE22_23970"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HSB17528.1"
                     /translation="MLYWILYEQLYPSIIPFPLFRYVTFRTAFSSLTALFLCIVLGPW
                     MINMLRRFQIGQHIREEGPRSHQKKAGTPTMGGLLILVSIVVPTLLWTNLRNPYVWIA
                     LFGLLSFGAIGFWDDYSKVRRKQNEGLTARRKFLLQVLAGLAVGVFLLSLHAMGLYST
                     TMNMPFFKGFKPDMLIDSLLGNPLTYPLAFVFFFAFLVFVIVGSSNAVNLTDGLDGLA
                     IGLMIIASGAMTVLTYASGHAQFASYLDLVRLPGASELTIFCGAMTGASIGFLWYNAH
                     PAEVFMGDVGSLSLGSGMATVAVLIKQEILLLFIGGVFVLEALSVILQVGSYKLRRKR
                     IFKMAPLHHHFEALGWQESKIIVRFWIAGLVTALFALTTLKLR"
     gene            complement(12369..13709)
                     /gene="murF"
                     /locus_tag="VLE22_23975"
     CDS             complement(12369..13709)
                     /gene="murF"
                     /locus_tag="VLE22_23975"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HSB17529.1"
                     /translation="MRFRLTEIAEAAGAGRPETQVEVTGWSVDTRTLQPGDLFFALHG
                     PNHDGHDYIADAVRRGASAVVAERAVDVAAPVVVVRDALKSLQQVACWARLRWGGQVV
                     GVTGSAGKTTTKEIIARMLGGAVAAGKTEGNLNNHVGVPLSILRLPEEARVAVLEMAM
                     NHAGELRELARIARPDVGVVTNVGHAHAEFFDSIEDVAHAKRELIEELGADGVAVLNA
                     DDPRVLRFRESHPGRTVTFGLSAEADVRGEDVEEGPDGVRFRAGNDRYESPLMGRHGV
                     LNLLAGIAVARHYGIPAEVQREAARSLEAGRMRGERFVHDGITVLDDCYNSNPEAVRA
                     MLDVLRVTPARRRFAVLGEMLELGRWSEALHRDVGRYAAGSGINVLVGIRGAARHMVD
                     EALKTGMSDSAAFFFAEPAEAGEVLHRLVREGDAVLFKGSRGTRVEQAMEIFLK"
     gene            complement(13706..15325)
                     /locus_tag="VLE22_23980"
     CDS             complement(13706..15325)
                     /locus_tag="VLE22_23980"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HSB17530.1"
                     /translation="MIRAGVRRKTENAGIGRTRRKAEERANSRLADAGRETGKAPVGS
                     MTLQEVLSGVRLRAALPPEVSRITVAGLEYDSRRIAAGELFFAFQGQNADGRSFAREA
                     VKRGAPAVVSELPKPEDFTGVWIEVEHGREAMALASRNFFGAPDERIVLTGITGTNGK
                     TTTSYLLDAVLRAAGKITALVGTIEYQVAGEVRRAVNTTPDSLDIVRILSELERKGGT
                     HATMEVSSHALALGRVYGVRFHTAVFTNLTRDHLDFHRTMEAYFGAKRTLFEGCGAPP
                     PRWAVLNEDDEYGRSIATAPGTERITYAIDRDAMVRARNIEAGLGGLTFAVETGNGRV
                     AVRSPLVGRINVYNILAAFGAGLAHGIDAEAIAAGIEQCRAVPGRFERVDEGQPFLVV
                     VDYAHTDDALRNVIRAGRELSPRRVITLFGCGGDRDRAKRPLMGAAAAELSDFVVLTS
                     DNPRSEDPLAIMNDAMVGLRRYDTPHVAEPDRERAIRRAIEEAGAGDLVLIAGKGHET
                     YQVLRDRTIPFDDREVVRRILRGFGYRREGA"
     gene            complement(15322..17361)
                     /locus_tag="VLE22_23985"
     CDS             complement(15322..17361)
                     /locus_tag="VLE22_23985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HSB17531.1"
                     /translation="MIRPASARFRFLGNRHPGSGDYGRQRLAVLVALAVLWIVAIVGR
                     LVYLQVFQHQTYRQLAEQQQRRRVELSAPRGAILDRNGQPLAMSLAVESVCINPRRAP
                     ELSVASGILAPILGLDRRELFGRMTEAAQRGRGFLWVKRKISLGEAERLRSLGLDWIE
                     FRRESKRSYPKGTLAAHVIGAVDHQERGNTGVEQTFDEVLRGRAGKARVLTDVLRRGI
                     LAQIAAEAQPGGDVVLTIDERIQHVAERELTKAVEGNNCVNGSVVVMNPYNGDILALA
                     SYPGFDPNDPIKTKTDVERRANHAFSVPFEPGSVFKVITVSAALETTTLRPGTIIPCG
                     NGRINLFGRVIRDHHSYSALPMADVLANSSNIGAIQVGLRVGERRLLEYVRRFGFGRM
                     TGLPLPGESAGMVRDLSAWGKSSIGSVAMGHEISTTTVQLARACSAIANGGLLVKPRL
                     VLTTRQPDRKAAPEPVEPPQRMIRPETAITMRQMMEGVVLHGTGKLARLDGYTAGGKT
                     GSAQIFDLVSKRYTHRYNASFLGFAPVTNPGVVVVVTLNGASKFGGAVAAPVFREVAT
                     AALRLLDVPRDLPDAPPSREDEPVEFDDLAIADLGSPAPMLPEPGIPDAHERVELWGP
                     KVPDFYGKSMRVVIAECSANGLPVELIGSGVARAQMPMAGSILPRGERVKIQFAR"
     gene            complement(17435..17926)
                     /locus_tag="VLE22_23990"
     CDS             complement(17435..17926)
                     /locus_tag="VLE22_23990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17532.1"
                     /translation="MATQATWYGRYGAVAENTTAVMPDAREERADIYRLRALPNEDVY
                     FYRKAIDNSRLIRQANPQASKRCLRMIATTCALTLLLLVMLWPKAYGVLAGYQIESLK
                     AQQQKLAVEHRSLEIEEARLLSPERIEEMAKTQEFIDPAPGQVVYLNPSADGSLAMNV
                     RSK"
     gene            complement(17939..18799)
                     /gene="rsmH"
                     /locus_tag="VLE22_23995"
     CDS             complement(17939..18799)
                     /gene="rsmH"
                     /locus_tag="VLE22_23995"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HSB17533.1"
                     /translation="MHVPVMLAEVLEFLAVRGDGTYLDVTAGLGGHTAAIAERLTTGA
                     AIACDRDAESLAMAEANTAQWAGRIRFRQARFSQLEAALTELGIAQVDGLVADLGVSR
                     YQLTDAERGFSISADGPLDMRMERSQQPTAAEIVNTIAEKALADLIFGLGQERRARKI
                     ARAIVRARPIRSTLHLARVVEQAAPRTGRLHPATQTFMALRMAVNDEQAELDRLLEAA
                     PRMVAGGGRIVVITFMSLEDRKVKQSFQGLARQGRAILLTKHVVRPTEEETRRNPPSR
                     SAKLRAVEMR"
     gene            complement(18806..19228)
                     /locus_tag="VLE22_24000"
     CDS             complement(18806..19228)
                     /locus_tag="VLE22_24000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17534.1"
                     /translation="MDEKGRLKLPAKLQQYISSFGSPRVFVTSLDTTTARIYPISIWK
                     ENEILLENYTEDPQAAEDIAFIANDLGADAEVDSQGRVLLPQELRQLLGVENQQVHLQ
                     WFKGRITLFTDNVYESRRAAARENLAGKLQGVEKGGLK"
BASE COUNT         3365 a         6726 c         5902 g         3630 t
ORIGIN      
        1 actgcgattt cacgccgtcg aagtgccggt gcggtggagc cacgttgagg gcaccaaggt
       61 gcggatgctc aaggacagca tgggtatgtt tgccgatctc gcctgcgtga ggtggaatca
      121 gctgcgcggg ctttaccggt agaggacgtt cttcctaccg ggttatgatg acttcccgca
      181 agtggtcccg cgccagaaag aacttgaccg agcggaactg gtggaacatc ttctcgatgg
      241 cgcggttgtt gaaatgctgg gctgcgcgcc tcgggccacg cggcgtgagc agcaaggtcc
      301 cggcatcccc gatgcggtaa ttgtacgagg ggatcgaaac ggccttctct tccgcgtgaa
      361 agaaggcgag aagattggcg cccggcttgg tgattcggtg gagccggtcg atcgtttcct
      421 tgagcagcgg cggggcgaga aattcgaggc aatcccagac catcgctccg tcaaaaaaac
      481 gatcctcaaa gtcgaggttt tcgtcaagaa accggccgat cctccgggca tcgaactgtc
      541 cggccaaagg atcgtcgccc gtcgcgtcga aggtggtgtc gagggttcgg aggaaatcga
      601 cggaatacag ccggtgcccc aggctcgtaa tgaagcccac gttcgcttgc gaagcgcccg
      661 cgaggtccag gatccacagg ttctggtcgc tctgaatgtg gctgaagaac tgctcaaggc
      721 cgtggctgtg ccgggagtag gacgtcccgg attcagtcgc tcgtacggac cgagaggaga
      781 cgatcggccc gcggggagac gccgccggcc gctccggtgc ggcagggccg cccacgaata
      841 tctttttcaa ccgttccttc agcaagaggg tggtcatccc cctaagacct acggcttcgg
      901 attcgcagag ccggtggcaa gcgtctgctg ctgcggagtg ccggtagtga cggcagcccc
      961 ctggggtttc ggcgcggggg cggctggttt actcacttcc gtcctggaaa tgtcgatgct
     1021 gcccttgaag taggcgccgt cttcgatgac gatgcgggac gtccgaatgt cgcccacgag
     1081 cttggcatcc ttacggatgt cgatcttgtc ggcggcttcg atgttcccat gcaccgtacc
     1141 gatcacgacc acttcgcgcg ccttgatgcc cgcctgaact tttccgttgg gcccgatcgt
     1201 gagccggtgc tcctggagct cgaccgtacc ctcgacctca ccgtcgatgt agaggtcttc
     1261 acggctgtat acttggcctt tcatcacaac cgacttaccg atgagcgcgc cgccacgcgc
     1321 cccctccggc tcggtgtttc ttgcagcgat ggtggacata ggaatgcctt cccgggccag
     1381 ctccgcggat gtgggagggg tggcctgcct ttgcgaatgg tcctcttctc tgcgcttcca
     1441 catggaggta tctcctccga ccgttcgagc acggtcgtcg tcgatcgtgg ctcgaccgaa
     1501 gtcctatact ataaagccgg catggaaaag gcaaaccatc gccgatagag gaggccgcta
     1561 tcgtgaaacc gacccggcgg agctgggctg agcccttcaa aatcaaggta gttgagccgg
     1621 tgcgtctgac gaccccggaa cagcggctgc gggccatcca ggaggcgggt tataacacgt
     1681 tccttctgcg ctccgaggac gtctacatcg accttctcac cgactccggc acgtctgcga
     1741 tgagcgaccg gcaatgggcc ggcatgatgc tcggcgacga ggcctacgcc ggcagtcgca
     1801 acttctatca cctcgaggag atggtccaga agtactatgg ctacaagtac ctgatcccga
     1861 cccaccaggg acgcggcgcc gagcatatcg tctcgcaggt gctcatcaag ccgggtgacg
     1921 tgattccagg gaacatgtac ttcaccacta cgcggctgca ccaggagctc gcggggggcc
     1981 ggttcgagga cgtcatcatc gccgaggcgc acgacccggc tagtgagcac ccgttcaagg
     2041 gcgacatcga cctcgataag cttgccgcgc tgatccgcga acacggccgg gaacatatcc
     2101 cctacgtgag cgtcgccgcg acggtcaaca tggccggcgg gcaaccgatc tccctcgcca
     2161 atctccaggc tgtgcgtgag cttacggcgc ggcatggcat ccggatcatc ctcgacgcca
     2221 cgcgggtggc ggaaaacgcc tacttcatcc agcagcggga acccggccag caggggaggt
     2281 cgattgcgtc gatcgtgatg cagatctgct cattgactga cggctgcacg atgagcgcga
     2341 agaaggatgc tttggtcaac atcggcgggt ttctcggtct caacgaccgg gacgttttcg
     2401 aggaggcccg caacctggtt gtcgtttacg agggattgca cacgtacggc gggatggcgg
     2461 gtcgcgacat ggaggcggtc gccgtgggaa tcgaggaatc gctcgacgac gaccatatcc
     2521 gggcgcgtgt cgggcaggtg ctgtacctcg gcggcaaaat gcgggagtgg gggatcccga
     2581 tcgttctgcc gattggcggc cacggggtgt tcatcgacgc aaggagtttc ctcccccatc
     2641 tgccgcagga gcagtatccg gctcaggcgc tggcgtcggc catctacctc gattcgggtg
     2701 tgaggacgat ggagcggggg atcgtctcgg caggccggga caaggaaacc ggacaggaga
     2761 acaaacccag gctggaactg gtccggctca ccattccccg ccgcgtctac acgcaggcgc
     2821 atatggatgt cgtggccgag tcggtgctga gcgtgttcga ggggcgggcc gccgttcgcg
     2881 gcctgcgaat ggtctacgag ccgcgctact tgcggttctt ccaagcccgg ttcgagccgc
     2941 tgtagacggt acgctgctct ccttctatct gtcgaagtgc agcctgtcgc ggcggaggta
     3001 tgcaggcgtg tcgaggtcgt cgccgatggc cggcggctcg gggacaggcg gctccgccgc
     3061 atcgggctcg ggttccggct cggccatggc cgccggaaat tccggctccg ccggcttctg
     3121 ggtgacggcc gccgccgcgc cgcgctcaaa tcccgtggcg atgacggtga ctttgaccgc
     3181 atctgccagc aactcgtcca acaccacgcc gaaattgatc tgaacgtcct cgcggcgcgc
     3241 ggcctcgcgg atgagcgagc atgcttcgtt cacctcgtgc agcccaagct tgtccgaccc
     3301 gctgatgttg accagcacgc tcccggcgcc ggcgattcca ccctcctgca tgagcggcga
     3361 gttgattgcc agccgcgcgg cctccaccgc ggcattctcc cccttcgcgg tggcggtgcc
     3421 catcagggcg taccccatcc cctgcatggt ggccttgatg tcggagaagt cgcggttgat
     3481 gagtccggtc ttggtaatga tgtcgctgat gccctgcacg gcctggcgaa cgacgtcatc
     3541 ggtcacgcgg aaggcgtccc ggaaacttgt tccccgtggc gccagcgaga gcagcttgtc
     3601 gttgtggatg gcgatgacgg tgtcgaccgt cgcggagagt tcctccagcc cacgcatggc
     3661 ctgccgcatc cgctgcgggc cctcgaagct gaacggcagg gtgacgacgg ccacggtgag
     3721 agcgccgagt tccttcgcca gcgagcagac gacgggtgtg gcgccggtgc cggtgccgca
     3781 gccgagcccg gcggtcacga acaccatgtc ggcgccttcg aggatatcca cgatgcgctt
     3841 ggtgtcctcg agcgcggcct cccggccgat ggccgggttc gacccggccc cgaggccgtg
     3901 cgtgagcttc gctccgatgg caagcttgtt gggcacaagc gccgcctcga gcgcctggtt
     3961 gtccgtgttg atgacatgga actcgacccc ttcgagtccc tcgctcatca ttcgggcgac
     4021 ggcgttcgat ccgccgcctc ccacgccgat gaccttgaac ctggtgccgg tgaccagctc
     4081 ctgatcgatc tcaaacttca agccttccgt ttcggtatcg agcgtcatcc tgtcctcctt
     4141 gcgggccgcg gcacgagcct cgccggccgg cccgcggcac tagtggaata atcgtcccag
     4201 aaaaccaccg ttctttcggt ccgcttccgt ccggtgcttc agccgggcgg agtacatcat
     4261 gaggccggcg gccgtcgtcc acgccggcgt atcgatatgt tcgggccagt cgagaatgcc
     4321 gaccggcagg ccgttacgcg cctggcaatt cagcacgcgt tccgccatgt cacacagccc
     4381 ggtgagctgc gccgtgcctc cggtgagcac gatgccgtct gccagcgcct gctccatgcc
     4441 gatcctcgcc agctcgcggc ggacaaaaag gaagagttcc tcggccctgg cctccaggat
     4501 catgttcagc tcgcgccgcg cggcctcacg cgcgggccgg ccctcgggcg acgggatctc
     4561 gatgatgctg ttgtcggcgg tcaggcccag gatcgcgcat ccgcattctt ccttgatcct
     4621 ctctgcctcc tcgtgggtga cgcaaaatcc aaacgaaacg tcgcgggtga aatgatcgcc
     4681 gctgatcgga agtccggtgg cgtgcaggag agcgtcgccg tagtacacga ccagatcagt
     4741 cgaatgcgcg ccgatatcca ccaccgcgac accctcgtgc cgctcgtccg gcagcaccgc
     4801 cgcatatgcc gccgctaccg gctcgtagac cgtctcctcg acggcgaggt gcgcccggtt
     4861 gaccgctccg accagagcat ggtgttcctg ggtcgaaatg gtgatgacgt gtacgaatga
     4921 ctcgagccgc gaccccagcg ctcctctcgg gttacggaag tccgcgcgcc catccaccga
     4981 gaagtcctgc gggaacatct ggagcaccat gcggtccgcc ggaagctgaa cgctcgacgc
     5041 ccgttcgacc gcgtaccgga gatcgacgtc ttcgatctgg cgcgggcgtc ccatttcgta
     5101 cagcccgcgg ttgttcaggc cctgaacggc gatgccgcct gcgcctacga cggccgcctg
     5161 cgccgataca cccgcctgct tttccgcctc cgcgaccgct agggcgaccg aaccggccag
     5221 cgccgccgga tcggcgatgc gacccttcga ccagccggcg gaatcggcct cgccgcaccc
     5281 aaccagccgc aggtgtgagt tctccgccag accgataaga cagcgcgtcc gcttgctgcc
     5341 cgcatccaag ccgacggcca atgttgcctc agctaccact gcctgccccc attgctgtga
     5401 tacggtcatc caaccgcagg tcgaatgcct tggcttgagc cagccggcgg cggatttcct
     5461 catagtgacc ccggaaattc tgcagtcgcg agaggaagtt tcgatcgccc agccacagca
     5521 cgactgcttc ccctccgatc tgctggacca ctttcaggtt gtccgtgtcg gagacatcga
     5581 tttcggaaac ttccgcggca agtccaccaa tgtcctgcac cagccgcatc gcgctctgca
     5641 cgcgcgccgc tcgcaccgca cggctctgac tccgtcggat gcccgacagt accggaagct
     5701 cgaaggtggc ccgctccggc tgttcgagaa tgacgccgtc tgcgtcgatc agcgccgccc
     5761 ggtgtacagc tcctctctcc ccagccggca attgcacgaa cgctaccgga gtccgttcct
     5821 tgattcgcac gatcagccgg tccggccaga gccgcgaaac cgaagcttcc cccacccagt
     5881 cgatcccgag aagctggagc cgccgttcac gcagcggcac aaggtacacg ctcctgccgg
     5941 tatcagcggc gaacattgcc agcactcgtg cccgggatgc gtgggccact ccctcgatcc
     6001 ggatgccgct gtcctggccg gattccgcgg tgtccttcag acgaaaccgc gggtcggtcg
     6061 ccagaaattg gtccacgcgg tagtacacgt agagcccgag cactgccagc gccagaatac
     6121 ccgccgcaac cgtcacatgc cggaataccg ccccccagtt ccggctgcgc ggcaccgatt
     6181 cggcgttttg tttccgagcc atgctagttc ctacctcgca ggagttcgag aagagcttct
     6241 cccacctgcc agacgttgcc cgctcccagc gtcagcacaa ggtcgccgtc gccggccgcc
     6301 gcgaataccg aggagacagc ccggtctatc ttccccgcgt actccgctcc gcggtgcccg
     6361 aaagcgcgga tgcgctccac cagcgcctcc gcggtgactc cttcgatcgg tttctccgac
     6421 gccgcgtaga tgtcgagcac gtacaccgtg tccgcttgat ggaacgcccg cgcgaaatca
     6481 tccatcaggt gcagtgtgcg cgtgtaacga tgcggctgaa agatcacgtg gactttccgg
     6541 taaccgccga ggcgggccgc ggcaagcgtc gcccggatct cggtcggatg gtgcccgtag
     6601 tcgtcgatca cgcttacgcc ccgctcccgg ccgcgcaact gaaaacgccg gtcgacgccg
     6661 ccgaactcgc cgagcgccgc ccgcatcgta tccggcttga cctccagttc gagccccacc
     6721 gcgacggcgg cggtggcgtc cagcacgttg tgcattccgg ccgccgggag atggaagcgc
     6781 cccaggtccg ctccgcggta gcgcaatgtg aagtcgctcg acgccggtcc gcagtcgatc
     6841 tcgccggcgg tcaggtccgc ctgcgcgctg gtcccgtacg tgaccactct gcggctgacc
     6901 gacggcagga tccgctgcac gttttcgtca tcgaggcaca gaatggccgc cccataaaac
     6961 ggcactttat tgacgaactc gacaaaagcc gcgagagtgt cctcgatcgt cgcgtagcag
     7021 tccacgtgtt cgcggtcgat gttggtgacg atcgccagta tcggcgccag cttaagaaag
     7081 gacctgtcgc tctcgtcgga ttccacaacc agaaaatcgc tccggcctac tcgggcgttg
     7141 gcgcccccca tggtccccac ccgtccgccg acgacgaccg tcgggtccaa acccgcgtgg
     7201 ctgagcaggg ccgcggtcat cgaggttgtg gtggttttcc catggctccc ggcgacggcg
     7261 atcccgtact tcagccgcat caactcggcc agcatctcgc ctcgctggat gatcgggatc
     7321 tgaagcctcc gggcctcttc aagttccggg ttccgctggt cgatcgcaga gctgaccacc
     7381 accgcttttg cgccgccgat gttttccgcg gcgtgtcctt ccgcgatccg cgcccctagc
     7441 ccggccagcc gcgcggtaat cggactgagc ttcgcgtcgg aaccgctgat ctggtagccg
     7501 aggttcagca gtacctcggc gattccgctc atgccgattc ccccgatccc gatgaagtgc
     7561 acatgctggg gtctgaaaaa catttttata tcgtattgtt tcggctttct gggtgccgtg
     7621 tcaacggttt tcgacaggct gctccgagtt cctcgagcag atctgcggcg cgctcggcgg
     7681 ctccgtggcg cgccaggccc cgtgccgcct gtcccatgcg ctccagcagc ccctcctctg
     7741 ccgccatctg cgccgtttcc cgaaacagcc gctcgccggt caactcggcg tcgggcacca
     7801 gcctcgccgc gcccgcgcgc tcgatcgcct cggcgtttcg caactggtgc tggtccgctg
     7861 cgaacggaaa gggaaccagg atcgccggct ttcccgcggc tgccagttcg gcgaccgccc
     7921 cggcgccgga acggcagacc agcaggtcgg cgcactcgaa cgccgccggc atgtcctcga
     7981 tgaagggcgt cacctctcca tcgagaccgg attcgtggaa gccacgctcg agttcggcgt
     8041 gatcttccgc tcccgtttgg tgaagcagcc gcaccggaaa ggaagcctcg cgaaagaacc
     8101 gccagctctc gcgagcggcc tggttgagcc tgcgcgagcc gcggctgccg ccggtgatca
     8161 acaccgtcag tctcgattct cttggtttcg gcgggaccgc gaagaactcg ccgcgcacgg
     8221 gcagtccggt cagctccgtc cgtcccactg gaaagccggc tgccgcctcg gggaagttca
     8281 acagcgcgcg ttcgacgaag cggcccatcc accggctcgt catgccgggg atggcgttgg
     8341 gctccataag cacgatcggg acgcgacgcc accatgccgc aagcatgact ggtcccgcaa
     8401 cgtatccgcc catactgaac accgccgccg ccttccttgc gcgaagtact gcagccgccc
     8461 gcgccgcgct tcgtggcagt tgcaaaagcg ttctcacggt ctgcccagcg ccgacgcgct
     8521 tcagcccccc gatgtcgatc cactcgatcg gaaaaccctc cgccggcgcc agtttcgcct
     8581 ccatcccgcg ccgggttccc acaaataccg cctggtgccc gcgcttccgc agttcccgag
     8641 ccaccgcgag cgcgggtatc acgtgaccgc ccgtcccccc gcccgccatc acgaaggtca
     8701 acccctcgct catccggaat gctcactcac gctctggagc atgccgaggg acaccagtgt
     8761 gctcagcagg gaactgccgc cgtagctgat catcggcagc ggaattccct tcggtggcac
     8821 catgcccagg acgacgctga cgttaaagaa cgcctgcaca acgatcatgg ccgtcacgcc
     8881 gagggcgaga tatcgtccga agtggtccgg cgcgcgaagg tagagtcgaa gccctctcca
     8941 aaggatgatc acaaagcccg ccagcacgaa caggcatccc aggattccga gttcctcgcc
     9001 gatgacggcg tagatgaagt ccgtatgcgc ttccggcaaa tagaagagct tctggtttcc
     9061 ctgcatcggg ccgagaccgg cggggccgcc ggagccgacc gcgatcagcg actggcgcac
     9121 gtgatagtcc gggtcccggc tcgccgccga tctctggagc acctgcttga acttgccctc
     9181 gggatcgatc atcttgacta ggcgcgtctc aagcagcttg tactcagggt cgacgtaggc
     9241 gaaaacccgc accatccggt acggcttcga gacgatggcg aggaggacgc agacgagcgc
     9301 gatgcccgcc gcggtcagga aatagcgccg ctccaggccg gctacataga agatggccgc
     9361 ggcagtggcc gtcagcacaa gcgccgtgcc caggtcggcc acgacgacac cgccggcgat
     9421 gagtcccacc atcatcgccg ccggcatcag cgtacctttg ttattgatgg ccgattcgcg
     9481 ccgggtcacg aagtaggcca ggaatacgat cagcgccggt ttggcgaatt ccgacggctg
     9541 gatccccatc gagccgatcc gcagccaccg gtgcgcgcgc gggtccacga agtacacgag
     9601 aagcagcgac gccagcacga cgccgaccga agcgaacgcc caccatgggc tgttgaaaat
     9661 ccggtagtcc cgccgcttca aaagcatcat tagaatcatc gcccccagcg cccagccaag
     9721 ctggcgcagg aagaagtagc cgctcgaccc gtacctgacc tccgcgacca cggaggacgc
     9781 gctgtacagc atcagcagcc caaacgcgac catcgccagg atcgtgaaga agaggatcca
     9841 gtcggtttgt actcgttgcg ccatcgtatc cctccggcgg gcgccccccg cctttacaac
     9901 tgttgcacca actccttgaa tacccggccg cggtgttcga aattctcaaa ctgatcgaaa
     9961 ctcgcgcacg ccggagcgag caaaaccgta tcgcccgccg acgccttcgc cgcggctctg
    10021 gatacggcgg ttgcgatagt gccgcactcg gccagtggca cggcgccgtg gagatgttcc
    10081 gcgatgatcg gcgtggccgc gccaacgagc aggaccgcct tcgctttcgc cgagagcggt
    10141 ccgccgagcg ggcgatagtc gctgcccttg tccttcccgc cgagaatcac ccacagcggc
    10201 cccgcgaacg cgtcgatcgc cttgagcgtc gcgtccacgt tggttgcttt cgaatcgttg
    10261 tagaaatcga tcccgcgcca cgtgcggaca aactccagcc tgtgctccac gcctggaaac
    10321 gtgcggaccg cgccggcgat cgcggacatt tgagcgcccg ccagccgcgc cgccgtggcc
    10381 gccgccaata cgttctcgac gttatgactg ccccgcagcg ggatcccggt tgcttcgaga
    10441 aaaggctcgc cttcggcata aagccagcct ccggccatcc agagaccggg ttccaccgga
    10501 tgcttcagac tgaaccagtg cgtccggccc gccgtccccc cggcgtatcc tgagcacacc
    10561 gggtcgtccg cgttcaaaac cgcaaaatcg tccggctgct gcgtccgaaa caggcggccc
    10621 ttcgccgccg cgtagttttc gaacgtgtgg tggcggtcca ggtgatccgg agtgacgttc
    10681 aacgccaccg cgatgtgggc gcgaaaccgt tccgtcgttt caagctgaaa gctcgacagc
    10741 tccagaacgt tccactggtc cggccgtgaa gtgtccacca tcccggcgac cgcgctgccg
    10801 atatttccgc ccacctgcac ggggattccg cactcgcgga gaatgtggcc ggtcagggcg
    10861 gtggtggtcg tcttgccatt cgacccggtg atgccgatga tctttccctg gaggaagtag
    10921 ccggcgagtt ccacctcgcc gatgacccgc accccgtggc ctctggcttc ttcgagaagg
    10981 tcgagatcgg ccggcacgcc cggcgacacg acaatccagt ccgcgtcccg gaaggcctcc
    11041 ggcgactggc ggacgaaccc ggcgcccgcg ctctccagta tccgcgccgc gccgggcagt
    11101 tcttcgagcg gcttcacgtc ggtggcggtg acgcgcgccc ccgtgcgcgc cagcaattgg
    11161 accagagcct ggcccgatcg gaccatgccc accaccacga ttctcgcgcc cgaaaaatcc
    11221 gtcgccttca tctcagtttc agcgtggtga gcgcgaacag cgccgtcacc agccccgcga
    11281 tccagaagcg cacgatgatc ttcgactcct gccatccgag cgcctcgaaa tgatggtgca
    11341 gcggcgccat cttgaagatc cgcttgcggc gcagcttata gctgcccact tgaagaatca
    11401 cggaaagcgc ctccagcacg aacacgccgc cgatgaacag cagcaggatc tcctgcttta
    11461 tcagcactgc gaccgtcgcc atcccgctgc cgagcgagag cgaccccacg tcgcccatga
    11521 aaacctcggc cggatgggcg ttgtaccaga gaaaaccgat cgaggcgccg gtcatggcgc
    11581 cgcaaaagat cgtgagttcc gatgcgcccg gcagccgcac caggtcgaga tagcttgcga
    11641 actgcgcgtg gccgctggcg tacgtgagca ccgtcatcgc tccggacgcg atgatcatca
    11701 gcccgatggc gagcccgtca agtccgtccg tcaaattgac ggcgttggaa gagccgacga
    11761 tcacgaaaac caggaatgcg aagaaaaaga cgaatgcaag cggatacgtc agcgggttcc
    11821 caagcagcga gtcgatcaac atgtccggct tgaacccctt gaagaacggc atgttcatcg
    11881 tcgtcgaata cagccccatg gcgtgcagcg acagcaggaa cacgcccacc gccagccctg
    11941 ccagtacctg gagcagaaac tttcgccgcg ccgtcaatcc ctcattctgt ttccggcgca
    12001 ccttagaata gtcgtcccag aatccgatcg cgccgaagct cagcagtccg aacagtgcga
    12061 tccagacgta cgggttgcgc agattcgtcc acaggagtgt cggcacgacg atcgaaacca
    12121 ggatcagcag cccgcccatc gtcggcgtgc cggctttctt ctgatgggac ctcggcccct
    12181 cttcccggat atgctggccg atctggaacc gccgcaacat gttgatcatc cagggcccaa
    12241 gcacgataca gagaaacagc gccgtcaggc tcgagaaggc ggtgcggaat gtcacgtagc
    12301 ggaacagggg aaacgggatg atcgagggat aaagctgttc gtaaagaatc cagtaaagca
    12361 tcggtgttct acttcagaaa tatctccatc gcctgctcga cccgagtgcc ccgcgagcct
    12421 ttgaagagaa ctgcgtctcc ctcccgcacc agccggtgca agacttcgcc ggcctcggca
    12481 ggttctgcaa aaaagaacgc ggcgctgtcc gacatgcctg tcttgagcgc ttcgtccacc
    12541 atgtggcggg ccgcgccgcg tatcccaacg agcacattaa tccccgaccc ggcggcgtag
    12601 cggcctacgt ctcggtgcag ggcctcggac cagcgtccga gctcgagcat ctcccccagc
    12661 acggcgaatc gccgccgagc cggcgtgacc cggagcacat ccagcatcgc gcggaccgcc
    12721 tccgggttgg agttgtagca atcgtccaat accgtaatcc cgtcgtgtac gaaccgttct
    12781 ccccgcatcc gcccggcctc gagcgatcgg gccgcctcgc gctgaacttc cgccggtatg
    12841 ccgtagtgcc tggcgacggc gattcccgcc agcaggttca acacgccgtg acgccccatc
    12901 agggggcttt cgtagcgatc gttgccggcg cggaacctca cgccgtccgg cccctcttcg
    12961 acatcctcgc cgcggacgtc cgcttccgcc gacaggccga aggtcacggt tcgccccgga
    13021 tgcgactctc gaaaacgcag aacccttggg tcgtcggcgt tcagaacggc cactccatcc
    13081 gcgccgagtt cctcgatcag ctcccgcttg gcgtgcgcga cgtcctcgat cgaatcgaaa
    13141 aactccgcgt gtgcgtggcc tacgttcgtc accacaccga cgtccggacg ggcgatgcgc
    13201 gccagctcgc ggagctcgcc ggcgtgattc atcgccattt cgagcacggc cacgcgggcc
    13261 tcttccggca gtcgcaggat tgacagcggt acgccgacat ggttgttcag gttgccttcg
    13321 gtcttgccgg ccgccacggc tccgccgagc attcgggcga tgatttcctt ggtggtcgtc
    13381 ttacccgcgc tgccggtgac cccgacgacc tgtccccccc atcgcagccg ggcccagcac
    13441 gccacctgct ggagcgactt cagcgcgtcg cggacgacta ccacaggcgc cgcaacgtcc
    13501 actgcccgct ccgccaccac cgccgaggcg cctcttcgta ccgcgtcggc gatgtagtca
    13561 tggccatcgt gattcggacc gtgaagcgcg aagaagaggt cgccgggttg cagcgtgcgc
    13621 gtgtcaacgc tccagcccgt aacctcgact tgcgtttcgg ggcggccggc gccggcggct
    13681 tcggcgatct cggtgaggcg gaacctcatg ccccctccct ccggtagccg aatccacgca
    13741 ggatgcggcg gaccacttcg cgatcgtcga acggaatcgt ccggtcccga agcacctggt
    13801 acgtttcgtg tcccttgccg gcgatcagca ccaggtcgcc ggccccagcc tcttcgatag
    13861 cccggcggat cgccctctcc cggtccggct cggcgacgtg cggcgtgtcg tagcgccgca
    13921 atccgaccat ggcgtcgttc atgatcgcca gcggatcctc cgaccgcgga ttgtccgagg
    13981 tgagcacgac aaaatcgctc aattccgctg cggcggcccc catgagcggg cgcttggcgc
    14041 ggtcgcggtc gcccccgcag ccgaacagcg tgatcacgcg gcggggtgaa agctcccgcc
    14101 cggcccggat gacgttgcgg agcgcgtcgt cggtatgcgc gtagtccacc accaccagaa
    14161 acggctgtcc ttcgtcgacg cgctcgaatc ggccgggtac ggcgcggcat tgttcgatgc
    14221 ccgccgcgat tgcctcggca tcgatcccgt gtgccagccc cgctccgaac gccgccagaa
    14281 tgttgtagac gttaatccgc ccgaccagcg gcgagcgcac cgctacgcgg ccgttcccgg
    14341 tttccacggc gaatgtcagg ccgccgagcc cggcctcgat gttgcgcgcg cgtaccatgg
    14401 cgtcgcgatc gatggcgtac gtgattcgtt cggtgcctgg cgcggtcgcg atggatcggc
    14461 cgtactcgtc gtcctcgttc agcaccgccc agcggggcgg gggagcgccg catccttcga
    14521 agagggtgcg cttggcgccg aaataggcct ccatggtgcg gtgaaaatcc aggtgatccc
    14581 tcgtcagatt tgtgaatacc gcggtgtgga atcgaactcc gtagaccctg cccaacgcca
    14641 gcgcgtggga ggacacttcc attgtggcat gggtgcctcc tttgcgttca agctcgctga
    14701 gaatgcggac gatgtccagc gaatcgggcg tggtgttgac cgcccggcgc acctcgccgg
    14761 cgacttggta ttcgatcgtt cccaccagag ccgtgatctt gcccgccgcg cgcagcaccg
    14821 cgtccagcag ataggaggtg gttgttttgc cgttcgtgcc ggtgattccg gtcaggacga
    14881 tccgctcgtc cggagcgccg aagaaattgc gcgaggccag cgccatcgct tcgcggccgt
    14941 gttcgacttc aatccacaca cccgtgaaat cctccggctt cggcaactcg gagaccaccg
    15001 ccggggcccc gcgcttcacg gcctcccgcg cgaacgacct cccgtccgcg ttttgtccct
    15061 ggaaggcaaa gaacagctcg ccggctgcga tccggcggga gtcgtactcc agccctgcga
    15121 ccgtgattcg agacacctcc ggcggcaatg ccgcccgcag gcggactccc gagaggacct
    15181 cctggagggt catgctccct accggcgcct tgccggtctc ccttccggcg tccgccagcc
    15241 gcgaattggc gcgctcctcc gccttccttc gcgtccggcc tatgcccgca ttctccgtct
    15301 tccttctgac tcccgcgcgg atcaccgcgc gaactgaatc ttcacccgtt ctccacgcgg
    15361 caggatgctg cccgccatgg gcatctgcgc acgggcgact ccgctgccga tcagttcgac
    15421 cggcagacca ttcgccgaac attccgcaat caccacgcgc atgctcttgc catagaagtc
    15481 ggggactttc ggcccccaca gctctaccct ctcgtgtgcg tctgggatcc ccggttccgg
    15541 gagcatggga gccggggacc ccaggtcagc gatagccagg tcgtcgaatt cgactggctc
    15601 atcttcccga gagggaggag cgtcgggaag atctcttggc acgtccagca gccgcagcgc
    15661 ggccgtcgcc acttctcgaa acaccggcgc cgccaccgcg ccgccgaact tggacgcgcc
    15721 gttcagcgtg actaccacca ccactcctgg attcgtcacc ggagcaaagc caagaaacga
    15781 cgcgttatag cggtgggtgt agcgcttgct gactaaatcg aaaatctgcg cggacccggt
    15841 tttgccgccg gcggtgtatc cgtccaggcg cgctagtttg cccgttccgt gcaggaccac
    15901 gccctccatc atctggcgca tcgtgatcgc cgtctccggc cgaatcatgc gctgcggcgg
    15961 ttcgaccggc tcgggcgccg ccttccggtc gggttggcgg gtcgtcagca ccagccgcgg
    16021 cttcaccaga agacccccgt tcgcgattgc cgagcaggca cgcgcgagct gtaccgtagt
    16081 ggtgctgatc tcgtgaccca tggccacgga cccgatggag ctcttgcccc atgcgctgag
    16141 gtctctcacc attcccgcgg attcccctgg cagcggcagg ccggtcatcc tgccgaatcc
    16201 gaacctccgc acgtattcga gcaatcgccg ttcgccgact ctcaagccca cctggatggc
    16261 tccgatgttg ctcgaattgg ccagcacgtc cgccatcgga agcgccgagt acgagtggtg
    16321 atcgcggatc acgcggccga acaggttgat gcgtccgttc ccgcacggaa tgatcgtccc
    16381 tggccggagc gtcgtcgtct caagcgccgc cgacaccgta atgaccttga acaccgagcc
    16441 gggctcgaac ggcaccgaaa aggcgtgatt ggcgcgcctt tcaacgtccg tctttgtctt
    16501 gatggggtcg ttcgggtcga agccggggta actggccagg gccagaatat cgccgttgta
    16561 ggggttcatc actacaacgc tgccgttgac gcagttgttt ccttcaaccg ccttcgtcag
    16621 ttcgcgctcc gccacgtgct ggatgcgctc gtcaatcgtc agcaccacat ctccgccggg
    16681 ctgggcctcg gccgcgattt gagcgaggat gccgcgccgg agcacgtcgg ttaatactcg
    16741 cgccttgccg gcccggcccc gcaggacctc gtcgaacgtc tgctcgacgc cggtgtttcc
    16801 tcgttcctgg tggtcgaccg ctccgattac gtgcgcggcc agcgttccct tcggatagct
    16861 gcgtttgctc tcccggcgga attcgatcca gtcaagcccc aggcttcgca gccgctccgc
    16921 ttcgccgagg ctgatcttcc gcttcaccca caggaaaccg cgtccgcgct gtgcggcctc
    16981 ggtcatcctt ccgaacagtt cgcggcggtc gagtccgaga atcggtgcca gaatgccgga
    17041 agccaccgaa agttccggcg cccgcctcgg gttgatgcag accgattcca cggccaggct
    17101 catcgccagc ggctggccat tccggtcgag aatagcgccg cgcggcgccg agagttccac
    17161 ccgtcgcctc tgctgctgct ccgccagttg tcggtaggtt tgatgctgga aaacctgaag
    17221 gtacacgagc cggcccacaa tcgccactat ccagaggacg gccagcgcca ctaacaccgc
    17281 cagccgctgt ctgccgtagt cgccgcttcc ggggtggcga tttcccaaga accggaatct
    17341 cgcgctggcg ggtctgatca tcggctcatc cgtcttggct gtttcgtgga tttggcgttc
    17401 tccgtgccac gcctagactc agcttccgct tcggctattt cgaccgcaca ttcatcgcga
    17461 gcgagccgtc ggcgctcggg ttcaggtaca ccacttggcc gggcgccggg tcgatgaact
    17521 cctgtgtctt cgccatctcc tcaatccgct ccgggctgag cagccgcgcc tcttcgatct
    17581 ccagcgaccg gtgctctacg gccagcttct gctgctgcgc cttcagggat tcgatctggt
    17641 atccggcgag caccccgtac gccttcggcc acagcatcac aagcagaagc agggtcagcg
    17701 cgcacgtggt cgcgatcatc cgcaagcagc gcttgctagc ctgagggttc gcctgccgga
    17761 tcagcctcga gttatcgatg gctttgcggt agaagtaaac atcttcattc ggaagggctc
    17821 gtaatctgta gatgtccgcc ctctcctcac gcgcgtcggg catcaccgct gtcgtgttct
    17881 ccgccaccgc gccgtatctt ccgtaccatg tcgcctgcgt cgccattgcc gactccagct
    17941 atctcatctc caccgcccgg agcttcgcgc tccgtgacgg cgggtttctc cgtgtttcct
    18001 cttcggttgg cctgacaacg tgcttcgtaa gcaggatcgc tcgtccctgc cgggccaggc
    18061 cttgaaaact ctgtttcacc ttgcggtctt ccaggctcat gaaggtgatc accacgatcc
    18121 gccctccgcc cgccaccatc cgcggcgccg cctccagtaa ccggtccagt tccgcctgtt
    18181 cgtcattgac cgccatcctc agagccatga aagtctgggt cgccggatga agtcgacccg
    18241 ttcggggcgc ggcctgctcc actactctgg ccaagtgcag tgtgcttcga atcggccgcg
    18301 cccgaacgat ggctctggca atcttccgcg ccctcctttc ctggccgagc ccgaagatca
    18361 ggtcggccaa tgccttttcg gcgatggtat tcacgatctc cgccgccgtc ggctgctggc
    18421 tgcgttccat gcgcatgtcc agtggcccgt cggccgagat cgaaaaacct cgttcggcgt
    18481 ccgtcaactg gtaacggctc acgcccaggt cggccaccag gccgtccact tgagcgatcc
    18541 ccagttcggt gagcgccgct tccaactgcg agaaccttgc ctgccggaac cggatacggc
    18601 cggcccattg cgccgtgttc gcctcggcca tcgcaagcga ctcggcgtcc cggtcgcagg
    18661 cgatcgccgc tccggtcgtc aatcgctccg caatcgctgc cgtgtggccc ccaagcccgg
    18721 ccgtgacgtc gagataggtg ccgtccccgc ggaccgccag gaattcgaga acctccgcga
    18781 gcatgacggg gacgtgcata catcactact tgaggccccc cttctcgacc ccctgaagct
    18841 tgccggccag gttctccctg gccgcggccc tccggctctc gtagacgttg tctgtgaaga
    18901 gcgtgatgcg gcccttgaac cactggaggt gtacctgttg gttctcgacg ccgagcaact
    18961 gccgcaactc ctgtggaagc agcacgcgtc cctggctgtc cacctctgcg tccgcgccaa
    19021 gatcgttagc aataaatgca atgtcttcag cagcttgggg gtcttctgtg tagttctcga
    19081 gaagaatttc gttttctttc cagatcgaaa tggggtaaat tcgtgctgtg gtggtgtcca
    19141 agctcgtgac gaagacacgg gggctgccaa agctgctaat gtattgctgc aactttgccg
    19201 gcagcttcag gcggcctttc tcgtccacac gcgccgaaaa cgctcctttt ggcgggtcgg
    19261 tttgcggcga aagattagaa attaactcca catcagaccc ttttaatcca cttcagtcca
    19321 cttgacagat tatgtaccag catcggcggg aatgcaaatc ttttttagag tgaagcccca
    19381 attctttcag cgatctagct gcacacaacg catggggacc tttcactctt cgcctctacc
    19441 tgttgcggta tgagtgaatt tctccctttc ggttcccctt gcagcctcat tccgcctaca
    19501 atcaatgacg atgcctgccc ctccgctctc tgaacgccac ctcgccctgc ttcgcgacta
    19561 cgacaccccg acgatttgca atgtcatcga gttattcgcc atccgtcctc gaagcaccgg
    19621 ctt
//
LOCUS       DATIFQ010000475        42518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2266333, whole genome shotgun sequence.
ACCESSION   DATIFQ010000475 DATIFQ010000000
VERSION     DATIFQ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 42518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42518
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266333"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1153)
                     /locus_tag="VLE22_24005"
     CDS             complement(137..1153)
                     /locus_tag="VLE22_24005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17535.1"
                     /translation="MKLVSILSVLALLGPVTGAAQAPQLIAESQPVVLDAGSAAYMFT
                     FMPRLAGGTVILEHRAGPSEPAYSIYSRDGKLQARFRIDLPDASETIDSRIAPLRDGS
                     GFVAGAIAVNGLDRAFKLCFLDRDGKLKKVVPISPYWPTHLAVAADGTIWGFGSASRD
                     DKPASDNPVVFHVSSSGELLGTALPRKLFGSEPPHELRPESGPAFLASGGSRVAVYSP
                     QTRQLFELGLDRKVIGSYVIPLPEQQRAGQSLARPMGLAGLAITDQGTIYAKLNGGDD
                     AGLYELDRPNQRWTPLPEELMNQVRAYSLIGCQGEDLALTPAGRRGQQFPVKWIRLTP
                     RFTP"
     gene            complement(1750..2877)
                     /gene="dnaJ"
                     /locus_tag="VLE22_24010"
     CDS             complement(1750..2877)
                     /gene="dnaJ"
                     /locus_tag="VLE22_24010"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HSB17536.1"
                     /translation="MQKRDYYEVLGVERNSSEQELKSAYRKAALKWHPDRNPGNHEAE
                     DRFKEAAEAYSVLSDSQKRAAYDRFGHAGVSGPAGAPGFDPSVFADFGDIFGDFDFFG
                     FGDLFGGGGGRRRRTRAQRGEDVGYNLDITFEDAVRGMSAEIQVPRMEACSRCEGKRS
                     EPGGLVTCPTCRGAGQVQYQQSFLSIRRTCSHCGGSGNIVREPCTQCRGEGYLRVERR
                     LKVNVPAGVDNGTRLRLSNEGQPGVNGGPPGDLYVLLRVEEHPFFERRDSDLHCTIPI
                     NVAQATLGAELEVPTLEGPEKLKIPEGTQHGAEFRMRNRGVPHVNGRGRGDLYFHIEV
                     KVPAKLTREQRRVFQELAGMLPSENEPKEKGILDKVKDYFM"
     gene            complement(2887..3471)
                     /locus_tag="VLE22_24015"
     CDS             complement(2887..3471)
                     /locus_tag="VLE22_24015"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSB17537.1"
                     /translation="MMVNSSTDENEGAAAGAAETGKGIPAVETEPGLDLAGQVAALTA
                     ERDRIAAEKAELYDRFVRRQAEFENFRKRVEREKGEIIAVAGMELARQLLPILDDFER
                     AIAHESTDSEYAKGIELIYQRLFETLQKAGLEPIVTEGQKFDPYVHHAMEMVETDEVE
                     DHSILAELLRGYNFRGKLLRPAMVRVAVAPSSGK"
     gene            complement(3542..4579)
                     /gene="hrcA"
                     /locus_tag="VLE22_24020"
     CDS             complement(3542..4579)
                     /gene="hrcA"
                     /locus_tag="VLE22_24020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HSB17538.1"
                     /translation="MTNKPQLTPRKAEILRNIVREYIETGEPVASRTISRRRKGSLSP
                     ASIRNVMADLCEEGYLAQPHTSAGRVPTEKAFQFFVREIGVPRPSPADYELLRAQLQG
                     ADTIEDGVARSSHLLTALTRSVSIVAAIPTASQTLEQIELVPLSDRRVLAVVATHDRA
                     VRHRVVTLDEAMSADELASIRNYLNYHFQGWVLSDIRRELEHRLEVESAYYDALHKRL
                     TVLYARGLLDIDLDPEVHLDGASYLVSLDLHLTRERMRELLHALEEKKRLLQLLDRFL
                     EQPAGAVGVCLGLGDLHPAMKELALIGMTVAMPSGMAAKVAVLGPMRMHYERVMAAVL
                     NVGRALQALPS"
     gene            4774..5178
                     /locus_tag="VLE22_24025"
     CDS             4774..5178
                     /locus_tag="VLE22_24025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17539.1"
                     /translation="MAIVRFILRSFSLLFHGAFALLLLGLSVVSFLTGAHTFEFYILP
                     WTGETLAYVLLVLAIAGVLIVLLAMRGTARMVFFIWSLLVLAMIVRGYFFSYYTFVPG
                     SGQFQWALWVLLAAVVAAIGARLEPGPAATRR"
     gene            5294..5908
                     /locus_tag="VLE22_24030"
     CDS             5294..5908
                     /locus_tag="VLE22_24030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17540.1"
                     /translation="MRFLALLAVALPLLAQSYPKHNLTVGIGAGLPGDDLSGLFKDSA
                     GVSVGYGYRFHPNFQLDGGLDVLFGSAGVRDFLPTEFGYLRIRDYQYLIPFGGRVILP
                     VGEGRLLLSAGGGGVHMRYSERLSQPSDFYRFKCPTCNSRNGWGYYALLGGSVALDRY
                     QRFRLGVTSKVYRGHTEGDPLGQVPGLRTRDRWVNLFGDFTISF"
     gene            complement(5930..6217)
                     /locus_tag="VLE22_24035"
     CDS             complement(5930..6217)
                     /locus_tag="VLE22_24035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17541.1"
                     /translation="MIRAVLYALLTIFLITFLRLVVGIITKAFGDLFVGESAREKQAR
                     PKTARGSETRGELKKDPVCGTYVATSTSVQKTIRGEVVHFCSESCRDKYSG"
     gene            complement(6260..7420)
                     /locus_tag="VLE22_24040"
     CDS             complement(6260..7420)
                     /locus_tag="VLE22_24040"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB17542.1"
                     /translation="MGKLSVKSAFWTAPALLLLFSGLSFAQTSSIEGKVIGDDGQPLK
                     DALIKIDRKDIKGNYKVKTKKKGDYFHAGLPLGTYVVTVEVGGQDKDRVDNVRTRLGE
                     PTVVNFNLHELKQKQEAMQKAAESGQLTADQQRQLSPEQKAELEKQMKERSAALAKNK
                     ALNDAFNVGMEALKTKQYQTAIDSFTKASELDAKQHVVWGQLAEAYVAFAATKTGAEH
                     DATLAKGLETFQKAIELKPDEAAYHNNFALALARAKKFDEAQAELTKAAEIDPPNGGR
                     YFYNLGAVLTNIGQLDPAGNAFKKATEMDPNYADAQYQYGVYLMSKAQTTADGKFVPP
                     PGTREAFDKYLQLKPDGPFAESAKGMLATMDQTIQTEYRDPDAKQKKTTKKK"
     gene            7581..8741
                     /locus_tag="VLE22_24045"
     CDS             7581..8741
                     /locus_tag="VLE22_24045"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB17543.1"
                     /translation="MEKGRLTRRNLLASAGGLAGLATVPVNAAGKHERLLITRIDLHR
                     VVVPMKPGTTYDPEVAESSRFDLVPKTIMKVHTDAGIIGIGETGRGEDEAGVRRNADY
                     LRGKHAFDLNLNRLDLPSRAGYAAFEMALYDAVGKALGWPVYKLLGGLAQRKVLVGYW
                     TGRKTPNGIRPVIEKTLAGGFTSIKTKCAQGDPVVQVAEVVAKMAPGVKLIVDPNTRY
                     RSYNDFLPVARDLDRVGNVLVLEDPFDKRDFNGYRELRRQVKAHVALHLGDPRAMVEA
                     IREQACSVFNTGGNPGMADFVGNCYLAQAAGMSVWHGSGNDLGIVDASYLHSCAASPA
                     CTLPSDILSFLREDDLIVDPIEIKNSYAIVSDRPGLGVDLDEEAVRRYAVKS"
     gene            complement(8731..9672)
                     /locus_tag="VLE22_24050"
     CDS             complement(8731..9672)
                     /locus_tag="VLE22_24050"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB17544.1"
                     /translation="MPLDPDVREYLDAQAASGAPPRGEMSVAETREFYLGMRPLAGEA
                     PSLPRVEDRVVPGPEGPVPIRIYAPATSRSLPVLVYFHGGRFFSGDLDTHDPVCRMLA
                     AEAGCLVAAVHYRLAPEHRFPAASEDCYAVTEWVARNAAEISADPEFVAVGGDSAGGN
                     LAAVTALLARQRGGSRICFQMLIYPMLDATRSLSSHTEYGSGYGPGSEDMERGWREYV
                     PPLTDRRHPQISPLWEADLGGLPPTFVLTAEYDSLRDEGEEYARRLAAAGVPVVLTRY
                     PGVIHGFFQLGGVVGMGREGVSDAARHLRRAFAAVRT"
     gene            complement(9674..10225)
                     /locus_tag="VLE22_24055"
     CDS             complement(9674..10225)
                     /locus_tag="VLE22_24055"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HSB17545.1"
                     /translation="MSKRILVVTGDGGEGYEALYACHRFLEAGHTPVVAAPSKRRLHL
                     VMHDFEPGWDTYIERPGYGLDADASIDEVSEGNFDAILLLGGRAPEYLRHNRKLVEMV
                     RLFHDSGKWVFAICHGIQILVAAGLAKDRCVTCYEHVRGEVELAGGFWSGQQAVRDGR
                     IVTAQTWQSHPEFYREIFHCLGE"
     gene            10224..10796
                     /locus_tag="VLE22_24060"
     CDS             10224..10796
                     /locus_tag="VLE22_24060"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSB17546.1"
                     /translation="MKPPLFVDMQILPAVHADGPVRRGILSPQEERGKVTSAGLIVFP
                     LLFALTTMAADLTEKKALTLAGAKHIAAAAEAEAVKNNWNVVIAIVDEGGHLIYLQRM
                     DDVQIASIDIAQRKARSAALFRRPTKAFEEQLAGGRVSVLKLEGAMPMEGGLPVIVDG
                     KVIGAVGVSGVTSQQDAQVAKAGLDVLAIK"
     gene            10843..13464
                     /locus_tag="VLE22_24065"
     CDS             10843..13464
                     /locus_tag="VLE22_24065"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB17547.1"
                     /translation="MSSHEPFHFHSLEELRRRIEELHLDLPVDDDLSPLLEPVRFGAV
                     PLPNRLVVLPMEGCDGKDDGSPDELTFRRYRRFTEGGAGLLWFEACAVVPEGRANPRQ
                     LWIHENNVAAFAKLVEDSLSGAHHAFGPSHRPFLVLQLTHSGRYSKPGRSPRPIIAHH
                     SRFLDPIHNLPPDYPLIPDDELERLEDVYVEAARFARKAGFDAVDVKACHRYLVSELH
                     ASFTREGRYGGSFENRSRFFRNVVAKIRDAVPGLVVTSRMNVYDAMAWPYGFGVDRDD
                     ASKPDLAEPIELVRHLAASGSPIVNITIGNPYFNPYVNRPFDLPVAGAPVPPESPLEG
                     VARFVSIVRRIQMEFPDMAVIGGGYSWLRHFFPNVGAACVRNGWVSLVGAGRMAFAYP
                     DFARDLRSRGSLDPERVCVACSACTQIMRDGGRSGCVPRDAAIYEPIYKAGRAEAVDT
                     ILAMAKTCRQCNDPTCVSGCPARVNIPRFVGHIADGRFREAYETLREANVLAAVCGYV
                     CPSETLCEAGCVNEHYTESVPIRHLQRWVSRKAVDEGWAAEPRPRETSTGKRVAVLGA
                     GPAGVSAAAALASRGHKVVLLDGSPAPGGTAAGTIPADRLPDSILRREIDDVLASSGD
                     VERRYNVCFDSNYGLDNVRGGGYDALLVALGLSGSVRLKTTVRPKSGVVGALEFLAAV
                     KRGGASSGVVLVLGGGNTAIDAALSAKRAGADDVSIVYRRSFAEMPAWPEERDRAIEA
                     GVNFLILTQPVGYVTDEQMKLAGLKVVRTRLSVAGKDGRRTVEPVPGSEHSMPADLVV
                     EAIGQQLGAELTAALAGMRFTADGLIWTRSGSFETSLPGVFAAGDIINGGTTVVQAVG
                     EGARAAREIDAWLRERP"
     gene            13461..15071
                     /locus_tag="VLE22_24070"
     CDS             13461..15071
                     /locus_tag="VLE22_24070"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HSB17548.1"
                     /translation="MRTLFPTAFASTLCVLASALAVSAAETVSLSRAEYEDRARAAWT
                     AQILGALMGFRFEHKPASTEWVDRLPKAYDHAPVDDDYYYEIVALRAFERYGTGLTVE
                     QLGAQWKENSAGSWGSSEQARLLLSRGVQPPDTGHPRYNKLWFTIGPQFSGDIYGVLA
                     PGLPNLAGRLARDLGHINGFAEGTDGGVFVAGMVSLAFAEDNSKEIVRKAARLIHPQS
                     PYRQCLDLVIGMAEKGAGPREIAQAVEDRWHIEYPATNNAVANGGLVALSVWFGEGDF
                     LKTVNLAFAAADYTDADCNAANAAAVVGAMHGMKALPLVLVEGIHDRIRGDAMGGVRL
                     TPAVDEAISDLARRTVAVGEKILRAEGVPADGDTLSIPAQDVATQPAELFSLADLTRY
                     WNPGWKLERVGFGGAGGGLGGVRGITYLDGDVLATYPRDEVRGALLRRTVRLGDQSSL
                     SVQVAADPGRAWQLDAFAGNERVLSRLIDGGQGKEREWRQIDVDLSRFANREVELRLF
                     QLVLLSGSIPGNAYWRALVVSEGLPRSK"
     gene            15187..16293
                     /locus_tag="VLE22_24075"
     CDS             15187..16293
                     /locus_tag="VLE22_24075"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSB17549.1"
                     /translation="MARVGFLFVGILSGLAFVCSLPAQPVRLVRHEIVSTRTAAIKGA
                     VLDLRKPGAPVLLTWGHGVDAHALLTGPNRAILASGGASFREGGCLFDIDGDSDPELV
                     LLEAGGDLVWIRQNGSVRGVIDTGVDSRDVVPATLHGRRGILLVQKQAQVRFYEIPKN
                     VRDKWPYREIYSFYTPSRQAGLRLDDVDGDGLPDILCGNYWIRSPASFDLPWRLFAIN
                     TWSETPDSAMLRLALADVAGRGSRQLIAAQAEVPAARLAWFEKPPDPTQLWAEHRVGG
                     VAGLRFLQGLDAADFDGDGLVDLAASENAGKRSLLFLLHNLGGGSFSTQRIASNCPVF
                     YLRATDVNGDTKPDLVTVGPGGAAWWENRTHLRK"
     gene            complement(16278..16625)
                     /locus_tag="VLE22_24080"
     CDS             complement(16278..16625)
                     /locus_tag="VLE22_24080"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB17550.1"
                     /translation="MKVYDWGNMTLEQMNPQIARQVIHAETMTVARIYLSKGGVVPEH
                     SHFNEQISMLQQGKLRFTIAGEERVLTAGEVIQIPPNAPHRVDALEDSVALDLFSPVR
                     EDWIRGDDAYLRK"
     gene            16708..18921
                     /locus_tag="VLE22_24085"
     CDS             16708..18921
                     /locus_tag="VLE22_24085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17551.1"
                     /translation="MLDSFSILRRLIVLTSLFALAAGAQGGKTRGWWPQPYNVQRDDA
                     AGKLVLSTPYYTIEHDLKQGGALSKIALTHGRAKNLLARPVDSSVQLSSPEGRPASER
                     EAPTANTYSDLFDASPDVSHSKDGDTEIVTVKASLVDKNGASSGVTVSTTYRYRWGYI
                     KIRKEFQFPETKRTRNVCVLNTVLDPSLTDYGYRPNVRELMDPELFSWRAGQIRQWGK
                     LRAGTHFDLPVQVRMLPRYFVFANPGVEGIEWFMSDNLYQWDYQMTGVPGTGWANAQT
                     STDPLGVAVSVYPVMLSSRYELPKGGAVTLKGSYTFDYYIGFPVLEGHAYKPWLNRSF
                     AVNKGEWVSEEEIKKNAADGVVTMHLHNDGDHHRDGMFWRDGSYPPYPPEEMKKMDAV
                     LKTIKKYGIKTAPYFSNHELHQSTEEFKKHGEEWGRIVEDQNNLRPNFYYGAHMCLKS
                     GWYDFLKFSIDRVLKNHHFDGTYYDWHIAMFCNNPRHVGKRSNGVSDEKGLGAPSISE
                     TTHWDIDELIGLAEWTRQRVGPDGIMILHNTLVPMFATENFANYIVGMEFSYGRLSVS
                     VPQPADVPLEWQFAGARPRTVIGYGTIAREAPKRLHRAHALLTLMTSVAPWPAGDEAL
                     DLFKALKPLGDIDRYQFEDYRNEAVKVDDRAVMHAVYSRAGEAWVLLGNAQPQPAAVR
                     VSVNAAKLRNPLASVKKAEVIVAGKTATLKPAKLSGAGHNITVPADDVVLVHLTN"
     gene            complement(18918..19445)
                     /gene="rsmD"
                     /locus_tag="VLE22_24090"
     CDS             complement(18918..19445)
                     /gene="rsmD"
                     /locus_tag="VLE22_24090"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HSB17552.1"
                     /translation="MRVIAGEFRSRRLKSLPGMRTRPTPDRLREALFSVLAPRIEGCV
                     FLDAYAGCGSVGIEALSRGARRAVFIEQNRAAARVIHENLDSLGVSERATVASGRVTA
                     LLERFEASVVFLDPPYEQEREYGKALEILGWTPPALAIVQHASRFALEEMYGRLRRTR
                     VLRQGDNSLSFYEPA"
     gene            complement(19445..21559)
                     /gene="recG"
                     /locus_tag="VLE22_24095"
     CDS             complement(19445..21559)
                     /gene="recG"
                     /locus_tag="VLE22_24095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HSB17553.1"
                     /translation="MRLPAVDLSTPVQYVKGVGPARAAMLETKGILTVEDLVGYAPFR
                     YEDRSNTKTIVQLAPGEMATVVATVRTTKLSGFQRRNLGLFEASFTDSSREILMGKWF
                     HGGYLKDVIAPGQKIALYGKIEYDSYSGRLGILHPEFEILTEDEEEGEAALHVGRIVP
                     IYEAAGKITTRIFRKLIYKALENLPALDEPLPPAVRARLKLMDRRTAVQQIHFPAAGE
                     DLRLLNAFRSPAQFRLIFEEFFWLECGLALKRSKARKRTGIGFELSENARQKIKRMLP
                     FRPTAAQKRVLGEIARDMAEPHPMNRLLQGDVGSGKTVVAAEAAIVAIENGYQVAVLA
                     PTEILATQHYLYFKKLFSGLGYVVVLLTGSATAREKSQLKKLAAEGLAHVVIGTHAVL
                     EENVEFKKLGLAVVDEQHRFGVMQRLRLMEKGTHPDVLVMTATPIPRTLALTIYGELD
                     VSVLDELPPGRKPIVTKHVTEDKVELVYSFLKQQVDAGRQAYVVYPVIEESETQAMKA
                     AQKMHKHLSEVVFPGLAVGLLHGRLPADEKEAVMEKFKKGEIKILVSTTVIEVGVDVP
                     NATVMVIEQAERFGLAQLHQLRGRVGRGASQSYCVLVTERLNDAAKERIRTMVESTDG
                     FYIAEIDLKLRGPGEFFGTKQSGLPALRIANILRDAEILEIARSEATAFIGSPPSEEE
                     LLRAVAYLRDHWQRRYGLVMVA"
     gene            21685..22620
                     /locus_tag="VLE22_24100"
     CDS             21685..22620
                     /locus_tag="VLE22_24100"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17554.1"
                     /translation="MKALVLLSTLSAAVSMAQIRVGIIGTDTSHVPAFTKVLNDPSSP
                     DHIPGARVVAAYKGGSPDVESSYTRVDKFAEEIRTKWNVELVKDIPTLCSKVDVVLLE
                     SVDGRVHLEQVKPVIAAGRPVFIDKPLAATLDEAREIARLAKQAGVPWFSTSSLRYSE
                     VATSMKYPDAKGVITWGPGPTEPHHYLDLSWYGIHAVEILYTLMGPGCEEVSRTSSPD
                     GDVIVGRWKDGRTGTVHSLRTSSEYGAVVFRPKQIEQSRPDMKSGYAPMLREIVKFFQ
                     TKQPPVPNEETLELFAFMDAAQRSKEAGGKPVRLR"
     gene            22654..23865
                     /locus_tag="VLE22_24105"
     CDS             22654..23865
                     /locus_tag="VLE22_24105"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HSB17555.1"
                     /translation="MTRREFGEWMMASGVLASTPLKAQETVEATGSHIGNLYPFVQKQ
                     ADQSKLELSFLRPEFRNLKQWKSRARARVFEQMFYSPPPVDPVPRQIRRTDRGDYIEE
                     YLNFQTTPDIRVPAFVLVPKNAKLPAPGIVALHDHGGFYLWGKEKLVPQDGEHPALTE
                     FRKRYYGGRSVPVELVRQGYVVIVIDMFYWGERRMLLDDDPPDFRERPSSMPQERINE
                     FNRRSGQNEQLVARSLFTAGISWPGVLLWDDIRTLDYLASRPEVDTNRLGCVGLSVGG
                     YRSFLLAALDERIKAAVDVGWMTSFASQIRTQVIHSVGLTFHLVGLYRYLDLPDLAAL
                     IAPRSLMVVNGSKDTLFAPGGVKAAFEKIDRIYRKAGAPDRQRCRLFDVPHEFNLEMQ
                     AEAFDWLKRWV"
     gene            23893..24663
                     /locus_tag="VLE22_24110"
     CDS             23893..24663
                     /locus_tag="VLE22_24110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HSB17556.1"
                     /translation="MSNLLEGKTALVLGVANKWSLAYSIAEAFRREGARVLLTYQGER
                     QRATVEALGAELGAARMLACDVTQDQELERLIETLRAEDGRLDAVVHSIAFANKEDLT
                     RPFVETSRSGFLLAHEVSAYSLVSVARATAPLMAGGGSLITLTYLGATRIITNYNVMG
                     VAKAALESAMRYLAGELGASGIRVNAISAGPIKTASARAIKDFSSVLDFVAQRAPLRR
                     NTEPAEVADAAVFLASDLGRGVTGNILFVDAGFQIVGL"
     gene            24775..25749
                     /locus_tag="VLE22_24115"
     CDS             24775..25749
                     /locus_tag="VLE22_24115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSB17557.1"
                     /translation="MIKVEGLTKRYARNVAVDNISFEVDKGSIVGFLGPNGAGKTTTM
                     RVLTCFLPPTAGGAEVAGFDVMKQPLEVKKRIGYLPEAPPLYPEMEVVEYLNFVANLK
                     GIPRGERKQRVDSVTERCAIGDVSTKLIGKLSKGYRQRVGLAQAIIHNPDVLVLDEPT
                     AGLDPKQILETRDLIKQLAGDHTIILSTHILPEVEQTCEKVIIISKGRLVATDSVQNL
                     TSRLRGAEAIAVEVEPRDTAPLDTVQVRQRLEHVPGVSRVVAKESKENRCIFEVESLQ
                     GRHIRADLARAVVEADWNLNELRPVAFSLEEIFLQLTSADRAAEGGEK"
     gene            25746..26513
                     /locus_tag="VLE22_24120"
     CDS             25746..26513
                     /locus_tag="VLE22_24120"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17558.1"
                     /translation="MRNVWTIYRKELKSYFASPIAYLLMAVFALIFGYFFYVATAIFV
                     SRGMESQMMGRGFPMDVNEWVIRPLMMNVSVIGLFMIPMITMRLFAEEKRSGTIELLM
                     TSPIRDVEIILGKWLAALTLYASILGLSMINVAVLFLYGKPDWKPILVGYLGLLLQGG
                     CLLAVGTFISTLTRNQIIAAGATFAACLLLWILDWVSAYETAAWAKVLSYMSVVTHFE
                     PFSKGVLDTKDVIFYLSVMFLGLFLTARSMESLRWRA"
     gene            26514..27890
                     /locus_tag="VLE22_24125"
     CDS             26514..27890
                     /locus_tag="VLE22_24125"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HSB17559.1"
                     /translation="MTFDWLKARQTKYTAYLTLYLAVILAVLGAANWLANRHNKSFDS
                     TANKRFSLSDQTEKVVKNLKQDVKIVYFDRTSDFTRARDLLDRYDNLSAKLTVAYVDP
                     DRKPQIAKSEGVRNYGTIFVETAGKREEAKSVSEEEITGALIRALKGGARNVCVVSGS
                     GEHSLDDTERSGYSGVKELLEKNNYKTRIVSLLEKPEVPKDCTILMVGGPRFDYVDAS
                     VNAIRAFVENGGRVLLMIDPPLKMGKEDIADNAALLKVLESWGVTPNKDLVLDTSGIG
                     QLFGLSEVVPLVTSYESHQIVREMKEIATAFPIARSLEVKSGDKTTVEKLFSTSGNSY
                     ATSSLSSSEIRINPAKDKKGPLTLAAAGTYRTGKEGGDGRFVVVGSSGWAANNILRFN
                     GNRDLLVNMMNWLSSDEDLISIRPKEPDDRRLTLTRRQMSTVFYSSVVGLPLAVVLAG
                     MWVWWRRR"
     gene            27894..29237
                     /locus_tag="VLE22_24130"
     CDS             27894..29237
                     /locus_tag="VLE22_24130"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HSB17560.1"
                     /translation="MRIRGLLIAVVVLAALAGGVYWSNKVKKAEEGKPSPDAPPKVLS
                     IPQDQFQQIEIHKGGVDALTLRKDDAGKWQMVSPKQLPVDQDAASSLVSSLSSLDSSR
                     LVEEKSADLAQFGLASPSLEVNVRQKDGKVTKLLVGDESPAGGGHFVKLDGDPRVFTI
                     ASYNKTSIDKTPADLRDKRLMTFDSDKLTRVELKAKGQSIEFGKNNQNEWQIIKPKPL
                     RADGGQVEELVRKLKDARMETTVSDADTKKAAAAFPGAKPVAVVRTTDAAGTQELDVR
                     KDKDNNYYAKTSVVEGVQKLTTFVGEGLDKGLDDFRNKKLFDFGFHDPSKLEIRDGGK
                     QVVYQKTGDKWMSGSKQMDGPSVQALVDKLRDLSAIKFLEQGSGSTVFEAAVTSNEGK
                     RLEKVIVSRQGDGFLARRENEPAVYELDGKAVEELQKAAAAVKELQPPKKEEKKK"
     gene            29242..30576
                     /locus_tag="VLE22_24135"
     CDS             29242..30576
                     /locus_tag="VLE22_24135"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HSB17561.1"
                     /translation="MKAIDGLLTDLYELTMSAGYFQAGKSEEKATFELTVRRLPKKRN
                     FVIVAGLEQVVEYLLELHITGGEIDYLRRLPQFANAPQGFFERLRDLRFTGDLFAAPE
                     GTVLFAGEPVLNVRAPLIEAQIPETYLLSMIGYQSMVATKAARIVETARGRGVVEFGT
                     RRAHSPEAGVLGGRAAYIGGCVGTSNTLSGYRFGIPVYGTAAHSWVQSFVDESESFRE
                     LQKLLGAFTIYIVDTYDTLEGTRRAAALGKPIQGIRLDSGDLGALARQARVILDEAGL
                     PDAKIMASGDLDEYKIRKLVNEGAPIDSFGVGTELVTSADAPNLGVVYKMVEVENGGV
                     KRYTAKFSEDKVTLPGAKQIFRYPDRDVIGRASECPPSASQVEALVRPVILGGSLVEP
                     LPGAAAVRERAAESLQRLPKACRRLRAPKPYHIEHSAKLLALAEQSRKGLTV"
     gene            30592..31170
                     /locus_tag="VLE22_24140"
     CDS             30592..31170
                     /locus_tag="VLE22_24140"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HSB17562.1"
                     /translation="MTTVFFDIDTQNDFMLPSGALYVPGAEAILPMIARLNRYAVHHG
                     IIVVSTTDAHDENDPEFRQWPPHCVAGTIGQQKPAETLLERRAVIPTTPGVYEIDGAQ
                     QIIVQKQALDLFTNPNLPALLERASRYVVYGVVTEYCVRCAVLGLLKTGKPVEMVTDA
                     IRTLKDDDGRRTIEEFTAAGGVLTTAERVCAG"
     gene            complement(31269..31670)
                     /gene="rsfS"
                     /locus_tag="VLE22_24145"
     CDS             complement(31269..31670)
                     /gene="rsfS"
                     /locus_tag="VLE22_24145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HSB17563.1"
                     /translation="MRSDKQAHETGKPEDENSELASPLWLAAVRAAESKKAVDIRVLD
                     LHEITSFTDFFVICTGTNPRQIQAISDEINIQLKRRGELPMSLEGYDNADWVLADYGD
                     FIVHVFSEKARVYYDLERLWRSAKPVELPEG"
     gene            31732..32253
                     /gene="hpt"
                     /locus_tag="VLE22_24150"
     CDS             31732..32253
                     /gene="hpt"
                     /locus_tag="VLE22_24150"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HSB17564.1"
                     /translation="MPQPSFRVLITAERIQERICELGARIDADYPEGPVYLVAILKGA
                     CFFLSDLARAITRPARIDFIGISSYAKGKTSSGEVKLTKDLDSSIEGAHVLIVEDIVD
                     SGITLNYLLKVLSQRKPRSLRIAALLDKPSRRIQPVDVAYSGFQIPDEFVVGYGLDYA
                     EDYRNLKDICVIE"
     gene            complement(32322..33371)
                     /locus_tag="VLE22_24155"
     CDS             complement(32322..33371)
                     /locus_tag="VLE22_24155"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HSB17565.1"
                     /translation="MKHTLLWKILAACAAFALLAACTSAPKKVEVDTAKLAMFKPLPA
                     VMESASNPLTEEKISLGRMLYYDKRLSKGQDVSCNTCHKLDNFGVDGEETSEGHLGKR
                     GDRNSPTVFNAAGHFVQFWDGRAPDVEEQAKGPVMNPVEMAMTSNKQVVAVLKSMPGY
                     VEAFKRAFPDDKDPVTFDNMAKAIGAFERKLVAPSRWDKFLQGDQTALTDVEKAGFNK
                     YMEAGCQACHAGPYVGAQMYQKIGVVKPWPDTSDEGRFKVTKQEGDKMMFKVPSLRGI
                     ERTGPYYHDGSIKTLDEAVSHMAEYQLGKQMTQEEIASIVAWLKTLTGEVPGDYIKEP
                     VLPPSTDKTPKPQKS"
     gene            33669..33815
                     /locus_tag="VLE22_24160"
     CDS             33669..33815
                     /locus_tag="VLE22_24160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17566.1"
                     /translation="MELIENGIEAEKQKQREFFELAERFRDATDPDEVKRLGDKLGRL
                     VFGD"
     gene            33824..34060
                     /locus_tag="VLE22_24165"
     CDS             33824..34060
                     /locus_tag="VLE22_24165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17567.1"
                     /translation="MESWDKFPAAVRQHLIERMRDREISVSDLNQLRLWVESKPEVPE
                     GDWYKDFGSFKVCGKGSFPKTFLLRGQPAKCLAL"
     gene            complement(34086..34667)
                     /locus_tag="VLE22_24170"
     CDS             complement(34086..34667)
                     /locus_tag="VLE22_24170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17568.1"
                     /translation="MKTTQMLLWSGLLAVGLCAASDVPTPAEQIASAVLAAPEELRSG
                     AAVLGYNAQGALVTLREGKNDLICLASDPGQKSFSAACYHRDLEPYMARGRELAAQKI
                     GGKERNEVRWKEVAEGKLAMPREPRTLYVLTGTGFDAATGKVTDAYLRWVIYVPFATP
                     ESTGLSTKPAENAPWLMYPGTAGAHIMINPSKK"
     gene            34824..35822
                     /gene="hemH"
                     /locus_tag="VLE22_24175"
     CDS             34824..35822
                     /gene="hemH"
                     /locus_tag="VLE22_24175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HSB17569.1"
                     /translation="MSGISASGRRIGVVLFQLGGPDSVDAVEPFLYNLFSDPDIIDFP
                     LASIARRPLARMIARARAHHVSRHYAEIGGRSPILEVTLRQAALLERELRRSLDARVV
                     VAMRYWHPLTGEAMEELRKHAPDEVVLLPLYPQYSRTTTGSSLNEWNRLSRLSDTWRP
                     KVHLVREYHADPDFIDALVAAVNESLAGFPHPITELDFLFSAHSLPLSVVQAGDPYQE
                     HVERTCDLVWARGGWPGRRHLCYQSKVGTSKWLGPSMEEAIERLAAEGARHVLVVPIS
                     FVSDHVETLYEIGIEHRELAERLGIADFRLMAGLNCSPHFIAALAKLVRQRLAVSV"
     gene            35948..36412
                     /locus_tag="VLE22_24180"
     CDS             35948..36412
                     /locus_tag="VLE22_24180"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSB17570.1"
                     /translation="MTLTDLSRIDRAIQIQAPPDRVWRALTDARELSAWFQVTIEGSM
                     EPGNEVWMTSVHPEHQGQRFRVWIVEMTPARRVVWHWHPGEVDPSVDYSREPRTTVTF
                     TLEPAAGGTRLSISETGFNEISLARRAKVYGDNAQGWTEVVVWLQKYAETGG"
     gene            36393..36734
                     /locus_tag="VLE22_24185"
     CDS             36393..36734
                     /locus_tag="VLE22_24185"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSB17571.1"
                     /translation="MPKRVVERNLAGVAVMFAALGDETRLLLLRRLSQGGPASISVLA
                     DRFRLTRQGVTKHLHVLADAGIIDGHRAGREHIWALNPTRLAEARRHLDVIARGWDDA
                     LTRLKTLIEGD"
     gene            complement(37212..38516)
                     /locus_tag="VLE22_24190"
     CDS             complement(37212..38516)
                     /locus_tag="VLE22_24190"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB17572.1"
                     /translation="MLAQDIAGDWLGTLHAGPMDLRFLVHITRGTDGILTGTMDSLDQ
                     NAKGIPIRSIELKESKLTFALAAIHYEGTVNKDFTAIEGTFTQRESFPLNFKRAGTEV
                     LAEPRRPQNPTQPYPYREEDLVYENRTAGIKLGATLTIPNRPGPFPAVILITGSGQQD
                     RDESLLGHKPFLVLADFLTRKGIAVLRSDDRGAGKSGGVFATATTADFATDTEAALAY
                     LKTRREIDPHKIGLIGHSEGGVIAPMVAARSKDVAFVVLMAGPGVPGDQIIVAQTVAG
                     GEAAGMPHEQAVQAGVKEREILDLLMKEKDEAVLKQKFKDLLGRQADVAYSQMTSPWY
                     RYFLSYDPAPALRRLSCPVLAINGEKDTQVPPKLNLPAIREALQDGGNKHFEAVEIPG
                     LNHLLQTAKTGAFREYGQIEETISPSALDKIGTWIAATVFGR"
     gene            complement(38633..39640)
                     /locus_tag="VLE22_24195"
     CDS             complement(38633..39640)
                     /locus_tag="VLE22_24195"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HSB17573.1"
                     /translation="MMIWIRLLPVMPQLFILAVLHALPLLPRRDHLFGLALAREVRYG
                     PEGRRLLRRYHLHLLPWTVSMLLIACFAPLPRAAAWVGFSLLPIIAAYRGFSAGRAEL
                     RGLALPAPSTREARLHADSGLARRLPWFLLPLALLAATAVYLHFRWDEIPARFPIHWG
                     QNGVPNAWSTRTPLGVFGPLLMGALLIVFIVAIDTVITWGSRRAAVHPVVRIILIAVG
                     WLVGAAFSIAGLLPLRVAPTWATVALNGAFFVFLAVVFTRHFRRGVPAPEPGAEVTPD
                     ACWHRDLFYYDPEDPALWVQKRIGLGLTLNFGNRLAWIVVAAFLLFPVGIVVLARALR
                     S"
     gene            complement(39637..40035)
                     /locus_tag="VLE22_24200"
     CDS             complement(39637..40035)
                     /locus_tag="VLE22_24200"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSB17574.1"
                     /translation="MTKRAPVIRIDLQSPVPQYRQIADALRTHLVDGNLTPGDTLPSV
                     RRLAMELGITFNTVAQAYRQLAGEGWLDLRHGRRVVVVDRQEPARASREAVVAFRQRL
                     REMVARMRAEGLSAGEIAAELRAAIERMTS"
     gene            complement(40051..40293)
                     /locus_tag="VLE22_24205"
     CDS             complement(40051..40293)
                     /locus_tag="VLE22_24205"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB17575.1"
                     /translation="MLSFWVFILADDLGWADTGVYGADLHETPNIDRLAAAGVRFTEA
                     CAAAPVCTPTRAAILTGKCQARLGMVLTNRYSNCTL"
     gene            40441..41196
                     /locus_tag="VLE22_24210"
     CDS             40441..41196
                     /locus_tag="VLE22_24210"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSB17576.1"
                     /translation="MKGVVLAGGTGSRLYPLTKITNKHLLPIYDKPMIYYPIQTLVDA
                     GIQEILIVTGGKNAGDFLRLLANGKEFGLSHLNYTYQEGEGGIAEALGLAEHFADGEK
                     LCVILGDNLIEGSIRHAVDDFRRQPKGAKVLLKEVPDAQRFGVAELVGDRIVGVEEKP
                     RKPKSNYAVTGFYMYDPTVFDKIRTLVPSDRGELEITDVNNAYVGEGTMSFAFLDGWW
                     TDAGTFDSLLRAANFVARSRHAQPEPAVAEATV"
     gene            41231..42271
                     /gene="rfbB"
                     /locus_tag="VLE22_24215"
     CDS             41231..42271
                     /gene="rfbB"
                     /locus_tag="VLE22_24215"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HSB17577.1"
                     /translation="MKLLVTGGAGFIGSAFVRLMIDAGASADILNLDKLAYAGNLENL
                     VAVAGHPRHNFVRGDVCDAACVEEQVSSFRPDAIVHFAAESHVDRSILSPAPVFETNL
                     RGTFTLLEAARKHHVPRFIQVSTDEVYGSIAEPLVADEKFPLRPSSPYSASKAGADLL
                     ALSYFTTYRVPVIVTRASNNYGPYQFPEKLIPLMIANAIADRPLPIYGDGLQVRDWLF
                     VDDHCRAVRAVLEKGRDGEVYNIGGNCSLPNREVVRRILAAAGKPETLMTPVEDRPGH
                     DRRYALTSEKLIRETGWQPEVGFEEGLARTIHWYLANQDWVRHVQSGEYLEYYTRNYG
                     DRAQELARLRKR"
     gene            42277..>42518
                     /locus_tag="VLE22_24220"
     CDS             42277..>42518
                     /locus_tag="VLE22_24220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17578.1"
                     /translation="MKVAIDATPLTLSSGGIARYTAELSVALASLFPEDDFVLLSDQP
                     FSMPAVGEKNGDTSGLRYSAAHLSRRADVSPVFSHPP"
BASE COUNT         7978 a        13516 c        12822 g         8202 t
ORIGIN      
        1 tttcaattga agctgttcga ccggctcgtc tggctctggc gccgcgtcga tccgctgcta
       61 ccttggccgc ccacctcgat catcgccgtc ggtaggaaac cgttgcgtca gcggaattcc
      121 gcctccagcg gctccgctac ggcgtgaacc gcggtgtcag gcgaatccac ttgaccggga
      181 actgctggcc ccttcgaccg gccggggtga gcgccagatc ctcgccctgg caacctatga
      241 gcgaataggc acggacctgg ttcattaact cttccggcag aggtgtccag cgttgattgg
      301 gccggtcgag ttcgtaaaga ccggcgtcgt ctccgccatt caacttggcg tagatggttc
      361 cttggtcggt gattgccagg cccgccaggc ccatcggtct cgccaggctc tggccggctc
      421 gctgctgctc gggcagtgga atcacgtacg aaccaatcac cttgcgatcc aaccccagtt
      481 cgaagagttg acgcgtctgc ggagagtaca cagccacgcg gctgcccccg ctggcgagaa
      541 aagcggggcc cgattcaggc cgaagttcat gcggtggttc ggagccgaac aacttcctgg
      601 gaagcgccgt tcccaacaac tccccgctgc tcgagacatg aaaaacgact ggattgtcgc
      661 tggccggctt atcgtcccgg ctggctgagc caaagcccca gatagtacca tccgcggcta
      721 cagccaggtg agtcggccaa tacggactga taggtacgac tttcttcagc tttccatctc
      781 gatcgagaaa gcagagcttg aaggctctgt ccaagccgtt cacggcgatt gcgccagcca
      841 caaagccgga gccatcccgc aggggcgcta tgcggctgtc gatggtttcc gaagcgtccg
      901 gcaggtcgat gcgaaaccgc gcctggagct tgccgtcgcg cgagtaaatc gagtaagccg
      961 gctcggaagg tccggcgcga tgttcgagga tgaccgtgcc gcccgccaaa cgcggcatga
     1021 atgtgaacat gtaggctgcc gaaccggcat caagtacgac aggctgggac tctgcgataa
     1081 gctggggcgc ctgcgcggcg cccgtgactg gccccagtag ggccagcacg ctgaggatac
     1141 ttaccaattt cacacctggc ctccttttct tcttcttcac aggtcgacgc taaacatagt
     1201 ctgggttatg gcaataagca tcccattctc ctaattcgat tacacgcgcc acacgccaga
     1261 acgtcaagaa cttctgttgt caaattctac tgaggttctt cggccgtttg aagccagcgc
     1321 ctatgctcgg acggtcgtcg gcccgagaac gttttgaaaa cctttacgaa ccggtcttcg
     1381 ctattgtagc caacctcgaa tgcgatctcc ttgatactca agtctgtatt cctcaacaga
     1441 tcttcggctc tttgcatcct cagcttcgtg acatacctgt gcgggctaag gcccgtatcc
     1501 tgcttgagaa cacgatagaa acgagaagtg gataggttag ctgccctcgc cagcattgct
     1561 acagtcagcg tctctcgata atggctatgg gtgaactgga tagccgcagc gattctcatg
     1621 tccacacatc cccgttgttc ttgtgacgcg gggcgtccag gcgtttcagg ttgtttcgaa
     1681 tgttatgacg aaaaagcgcg ccttgccgtg caggcaagga tgaacgggag gcaaatcact
     1741 cgccaagtct tacatgaagt agtctttgac cttatcgaga atccccttct ccttcggctc
     1801 gttctcggag ggcaacattc ccgcaagttc ctgaaacact ctccgctgct cgcgggttag
     1861 tttggccggc actttcactt cgatatggaa gtacaggtcg ccccgtcccc gcccgtttac
     1921 atgcgggaca ccgcggttgc gcatgcggaa ctcggcgcca tgctgcgtcc cttcggggat
     1981 cttgagcttc tccggaccct cgagcgtcgg cacctcaagt tcggcaccca gcgtcgcttg
     2041 cgcgacgttg atcgggatgg tgcagtgcag atcgctgtcc cgcctctcga agaacggatg
     2101 ctcctcgacc cggagcagca cgtacagatc ccccggcggc cccccgttca ctccaggctg
     2161 cccttcgttg gacagccgca ggcgggtccc gttgtccact cccgcgggta cgttgacctt
     2221 cagcctgcgt tccacccgca gatagccctc tccccggcac tgagtgcacg gttcgcgaac
     2281 aatgttcccg ctccccccgc aatgggaaca ggtccgccgg attgagagga agctttgctg
     2341 atactggact tgccccgcgc cccggcacgt cgggcacgtc acgagcccgc cgggttccga
     2401 gcgtttgccc tcgcagcggg aacaggcctc catgcgcggc acctgaatct ccgcgctcat
     2461 gccgcggacc gcgtcctcga acgtaatgtc gaggttgtag ccgacgtcct ccccgcgctg
     2521 cgcgcgcgta cgccggcggc ggccgccgcc accgccgaac aggtcgccga atccgaagaa
     2581 atcgaagtcg ccgaaaatgt cgccgaagtc cgcgaaaacg gacggatcga agcccggagc
     2641 tccggccgga ccgctcacgc cggcatgacc gaaccggtcg tatgcggccc gcttctgcga
     2701 atcgctcagc acgctgtacg cttcggcggc ttccttgaag cggtcctcgg cctcgtgatt
     2761 gcccggatta cggtcggggt gccacttgag cgccgctttt cggtaggcgc tcttcagctc
     2821 ctgctcggaa gagtttcgct cgacgccgag cacttcgtag tagtctcgtt tttgcacgct
     2881 tgccacttat tttcccgaag agggagcgac ggccacccgg accatcgccg gccgaagcaa
     2941 cttaccccga aagttgtatc cgcgaagcag ttccgccaga atcgaatggt cctctacctc
     3001 atcggtctcc accatctcca tcgcgtggtg gacgtaagga tcgaacttct gcccctcggt
     3061 gacgatcggc tccagccccg ccttctgcaa agtctcgaat aatcgctgat agatcaactc
     3121 gatcccttta gcgtattccg agtcggtgct ctcgtgcgcg atagcgcgct cgaagtcgtc
     3181 caggatcggc agcaattgtc gagccagttc cattccggcg acggcaatga tctcgccttt
     3241 ttcgcgctcg acacgtttcc ggaagttctc gaactcggcc tggcgccgca caaaccggtc
     3301 atacaattcc gccttctcgg ctgcaatccg gtcccgttcg gcggtcagcg ccgccacctg
     3361 gccggcgagg tccagcccgg gctccgtttc taccgcggga atgcccttcc cggtctccgc
     3421 cgctccggcg gcggctcctt cgttttcgtc agtgctgctg ttcaccatca tgtcgtttct
     3481 ttacaggtta gcaaagcgga atcggccgca ccgagtgaaa ccccgcggcc tggaaaaagc
     3541 ttcaggaggg aagggcctgc aacgcccggc cgacgttcag aacggcagcc atcactcttt
     3601 cgtagtgcat tcgcattggc cccagcacgg cgacctttgc ggccatcccg ctcggcatgg
     3661 ctaccgtcat gccgattaac gccagctcct tcatcgccgg atgcaggtcg cccagaccga
     3721 gacacacgcc aacggccccg gccggttgtt caagaaaccg atccagcaac tggagcaggc
     3781 gcttcttctc ctcgagcgcg tgcaacagtt ctcgcatcct ctcccgggtc aggtgcaggt
     3841 cgagactgac caggtaagag gccccatcca ggtgaacttc cggatcgagg tcgatgtcca
     3901 gcaggccgcg tgcgtaaagc acggtcaacc gcttatgcaa agcgtcgtag taagcgctct
     3961 cgacttccag ccggtgctcc agctcgcggc gaatgtcgct cagtacccat ccctgaaagt
     4021 gatagttcag atagttccga atggacgcca gttcgtccgc cgacatcgct tcgtccagcg
     4081 tcaccacccg gtgccgcacc gcgcggtcgt gcgtcgccac gacggccagt acccgccggt
     4141 ccgaaagcgg caccagttcg atctgctcca gcgtctgact ggccgtcgga atcgcggcca
     4201 cgatgctgac gctgcgcgtc aatgccgtga gcaggtgcga agagcgggcc actccgtcct
     4261 cgatggtatc ggcgccctga agctgcgcgc gtaatagttc gtaatccgcg ggcgacgggc
     4321 gcggcacgcc gatctcccgc acgaagaact ggaaagcctt ttcggtcggg accctgccgg
     4381 ccgacgtgtg cggctgcgcg aggtagcctt cctcgcacaa gtcggccatc acgttccgga
     4441 tggatgccgg actcaacgac cccttgcgcc gcctggaaat ggtccgcgaa gcgacgggct
     4501 cgccggtctc aatgtattcc cgaacgatgt ttcgcaggat ctccgccttg cgaggcgtca
     4561 actgcggctt gttcgtcatg ctctttcgtt cagggtcagt ccccttgatt cgtattctag
     4621 aggcgcggaa ccatagggtc aaggaataac gccgggcggc tgccccctct tgcgcacacg
     4681 tcaccgcccc ggtcacggtg tccgcatcca gcgagtcacc ctgctacaat gtcaacttac
     4741 tgaaatcaga tcgattattc cgggagacct ttcgtggcta tcgtacggtt cattctccga
     4801 tccttcagtc ttctcttcca tggagcgttt gctctgttgc tgttgggtct gtccgtggtt
     4861 tcgttcctga ccggcgctca cacgttcgag ttctacatcc ttccatggac aggtgaaacg
     4921 ctcgcctacg tcctgcttgt cttggccatc gccggcgttc tcatcgttct gctcgcgatg
     4981 agagggactg cacgaatggt gttcttcatc tggagcctcc tggtcctggc gatgatcgtg
     5041 cgcggctact ttttttcgta ctacaccttc gtgccgggat ctggtcagtt ccagtgggcg
     5101 ctgtgggtgc ttcttgcagc ggtggtcgcc gccattggcg ctcgcctgga acccgggcca
     5161 gcggccactc gccgctaaga tgaagcctta ttaagctaga gcgctcgctc gaagttggct
     5221 ctggctgcac ggaacaacgg cagtcgaacg cggctggaac ctgccatccc caggccgcgt
     5281 cggacggagt ggtatgagat tccttgcgct gctggctgtg gcgctgcccc tgctggcaca
     5341 gtcttatcca aaacacaatc tcacagtcgg catcggtgcc gggttgccag gcgacgatct
     5401 gagtggcctg tttaaggaca gcgccggcgt ttcggtcggc tatgggtacc gcttccaccc
     5461 caacttccag ctcgacggag gactggacgt actcttcggc tccgcgggag tccgggattt
     5521 tctgccgact gagttcggct accttcgcat ccgcgattac caatatctga tcccctttgg
     5581 cgggagagtc atcctccccg taggagaagg gagactgctc ctctcggccg ggggcggcgg
     5641 ggtccacatg cgctactcgg agcggctcag ccagccgagt gatttctacc ggtttaagtg
     5701 cccgacgtgc aattcccgta atggctgggg ctactacgcc cttctcggcg gcagtgtcgc
     5761 gctagacaga taccagcgct ttcgccttgg cgtgacctcc aaggtctacc gcggccatac
     5821 cgagggagat cctctgggac aggttcctgg acttcgcacc cgcgaccgct gggtcaacct
     5881 gttcggcgac ttcacgatct ctttctgaga tccgtcaggg aaatgcggct cagccgctgt
     5941 acttgtcgcg gcaggactcc gaacagaaat ggaccacttc gccgcgaatg gtcttctgga
     6001 cactcgtcga agtggcgacg tacgttccgc aaaccggatc cttcttcagt tcgccgcggg
     6061 tttcggaccc tcgtgcggtc ttcggccggg cctgtttctc gcgagcgctc tcgcccacaa
     6121 aaaggtcacc gaaggccttg gtgatgatgc ctactaccaa acgcaggaag gtaatgagga
     6181 agattgtgag gagagcgtaa aggacagcgc gaatcatgag acggggcgcc ctccgcgagc
     6241 gccccgggaa aactggctct tacttcttct tcgtcgtctt cttctgcttg gcgtcgggat
     6301 ccctgtactc ggtctggatc gtctggtcca tcgtcgccag catgcctttc gccgactcgg
     6361 caaacggccc gtccggtttc agttgcaggt acttgtcgaa cgcttcacgc gttcccggcg
     6421 gcggaacaaa cttgccgtcg gccgtggtct gggcctttga catcagatac acgccgtact
     6481 gatactgcgc gtcggcgtag ttcggatcca tctcggtcgc cttcttgaac gcattgcctg
     6541 caggatcgag ctgcccgata ttggtgagca ccgcgccgag gttatagaaa taccggccac
     6601 cgttcggcgg gtcgatctcg gcggccttcg tgagctcagc ctgtgcctcg tcgaacttct
     6661 tggcgcgggc cagtgcgagc gcgaagttgt tgtgatacgc cgcttcatcc ggcttcagct
     6721 cgatggcttt ttggaacgtc tccaggccct ttgcgagcgt ggcatcgtgc tcggcgccgg
     6781 tcttcgtggc cgcgaaggcc acataggctt ccgcgagctg tccccacacc acatgctgtt
     6841 tggcatcgag ttcgctcgct ttggtgaagg agtcgatcgc cgtctgatat tgctttgtct
     6901 tcagggcctc catgccaacg ttgaaggcgt cgttcaacgc cttgttcttc gccagtgcgg
     6961 ccgaacgctc cttcatctgc ttttcgagtt cggctttctg ctccggcgaa agctgccgct
     7021 gttggtcggc tgtcaattgt ccggactcgg cggccttctg catcgcttcc tgcttctgct
     7081 tgagctcatg gaggttgaag ttgacgacgg tcggctcgcc cagtcgcgtg cggacgttgt
     7141 cgacacggtc cttgtcctga ccgcccactt ccaccgtcac cacgtacgtg ccgagcggca
     7201 atccggcgtg gaagtagtcg cccttcttct tcgtcttgac cttgtaatta cctttgatat
     7261 ctttccgatc gatcttgatc agcgcatcct tcagcggctg accgtcgtcg ccgatcacct
     7321 tgccctcgat cgatgacgtt tgcgcaaacg acagccccga aaacaacagc agcaaggctg
     7381 gagccgtcca aaacgcactc ttaacactga gcttacccat ataaacagat ctccggctct
     7441 gaatgagagc ttcaactaca gcatactaca tccgttatgg ggccaagcct cggaaagctc
     7501 gcgcgaacag gagcccttcc ggcgttatat actgtgaccc gcgccgcacg tgacaagccc
     7561 gcgggcgagg aggcaacgac atggaaaaag gtagactgac tcggcggaat ttgcttgcgt
     7621 ccgcaggcgg attggccggc cttgcgacag tgccggtaaa cgccgccgga aagcacgagc
     7681 gcctgctgat cacacggatc gacctgcacc gcgtcgtagt gcccatgaag ccagggacca
     7741 cctacgatcc ggaggtcgcc gagtcgagcc gtttcgacct cgtcccaaag accatcatga
     7801 aagtccacac cgacgccggg atcatcggaa tcggcgagac ggggcgtggc gaggacgagg
     7861 cgggtgtccg gcgcaacgcc gactatctgc gtggcaagca cgccttcgac ctcaacctca
     7921 atcggctcga tctgccgtcc cgcgccggct atgccgcctt cgaaatggct ttgtacgatg
     7981 cggtcgggaa agccctaggc tggcctgtgt acaaactgct gggcggcttg gcgcagcgca
     8041 aggtgctcgt cggctactgg accggccgca aaacccccaa cgggattcgg cccgttatcg
     8101 agaaaacgct cgccggcggc ttcacctcca tcaagacaaa gtgcgcgcag ggcgatcccg
     8161 tcgttcaggt ggccgaagtt gtggcaaaga tggcccccgg agtgaagctg atcgtcgatc
     8221 cgaacacgcg ctatcggagc tacaacgact tcttgccggt ggccagggac ctggatcgcg
     8281 tgggcaacgt gcttgtcctc gaggacccat tcgacaagcg tgacttcaac ggctaccgag
     8341 aactccggcg gcaagtgaaa gcgcacgtgg cccttcacct cggagacccg cgggcgatgg
     8401 tcgaagccat ccgcgaacag gcctgctcgg tattcaatac gggcgggaat cccggcatgg
     8461 cggacttcgt cggcaactgt tacctcgccc aggcggccgg catgtccgtg tggcacgggt
     8521 cgggcaacga cctcggaatt gttgacgcct cctacctgca ttcctgcgct gcctcgccgg
     8581 cttgcacgtt accgagtgac atcctgagtt tcctgcgtga ggacgacctg atcgtcgatc
     8641 cgatcgagat caagaacagc tacgcgatcg tctcggatcg accgggcctc ggcgtcgacc
     8701 ttgatgagga ggcggtccgc cgctacgctg tcaagtcctg accgcggcga aagcccgcct
     8761 gagatgcctc gctgcatcgg agaccccttc gcggcccatc ccgacaactc cgccaagctg
     8821 gaagaatccg tggatgacgc cgggatagcg agtcagtaca accggcacgc cggcagcggc
     8881 taaccgccgc gcgtattcct cgccctcgtc gcgcaggctg tcgtactcgg ccgtcagaac
     8941 aaacgtcggc ggcaggccgc ccagatccgc ctcccacaac ggcgaaatct gcgggtgcct
     9001 ccggtccgtc aatggaggca cgtactcgcg ccagccccgc tccatgtcct cacttcccgg
     9061 accgtagccc gagccgtact ccgtgtgcga agacaggctg cgcgtcgcgt cgagcatcgg
     9121 gtagatcaac atctggaaac aaatcctcga gccaccgcgt tggcgtgcaa gcaatgctgt
     9181 caccgccgcg agatttccgc ccgcgctgtc gccgccgaca gctacgaatt ccgggtccgc
     9241 gctgatttcc gccgcgttcc gcgcgaccca ttccgttacg gcgtaacaat cctccgaagc
     9301 ggctgggaaa cggtgctccg gagcgagtcg gtagtgaacc gctgccacaa ggcagcccgc
     9361 ttcggccgcc agcatgcggc acacaggatc gtgcgtgtcg agatctccgc tgaaaaaccg
     9421 gccgccgtga aagtacacga ggaccggaag actgcgactc gtcgccggcg cgtaaatgcg
     9481 gataggcacc gggccctccg gcccgggcac gactcggtct tcaacgcgcg gcagcgaagg
     9541 cgcttcaccc gccagcgggc gcattccgag atagaactca cgcgtctcgg cgacgctcat
     9601 ctcgccccgt ggcggcgccc cggacgcggc ctgcgcgtcg aggtattcgc gcacatccgg
     9661 atcgagcggc atgctactcc cccagacagt ggaagatctc gcgatagaac tcggggtgag
     9721 actgccacgt ttgtgcggtc acgattctgc cgtctcggac cgcctgctgt ccgctccaga
     9781 agcctccggc cagttcgact tcgccgcgca cgtgctcgta acatgtgacg cagcggtcct
     9841 tcgccagccc ggccgctacc aggatctgga tcccgtggca gatcgcgaag acccatttcc
     9901 cgctgtcgtg gaataaacgg accatttcga ccagtttccg gttgtgccgc agatactccg
     9961 gtgcgcggcc acccagaagg agtatggcgt cgaagttacc ctccgagacc tcatcgatcg
    10021 atgcgtccgc atccagaccg tagcccgggc gctcgatgta cgtgtcccag cccggctcga
    10081 aatcgtgcat gacgagatgc agtctccgct tcgacggagc agccaccacc ggtgtgtggc
    10141 ccgcctcgag gaaacggtgg caggcgtaca gcgcctcgta accctcgcca ccgtccccgg
    10201 tgacaaccaa aatccgtttg ctcatgaagc ctccgctgtt cgtggacatg caaattctac
    10261 cagccgtgca tgcggatggt ccggtccgcc gtggtatcct cagcccacag gaggaacgcg
    10321 gaaaagtgac cagtgccgga ctcattgtat tcccactttt gttcgcgcta acgaccatgg
    10381 cggcagatct cacggaaaag aaagcgttga ccctcgctgg agcaaagcac atcgcggcgg
    10441 cagccgaagc ggaagcggtg aagaacaact ggaatgtcgt catcgccatc gtcgatgagg
    10501 gtggtcacct catctacctt cagaggatgg acgacgtcca gatcgccagc attgacatcg
    10561 cgcagcgcaa agcgcgcagt gcggcgctct tccgccggcc gacgaaagcc tttgaggaac
    10621 agcttgcggg cggtcgcgtc tccgtcctga aactcgaagg cgccatgccc atggagggcg
    10681 ggcttcccgt gatcgtggac ggcaaggtca tcggagccgt cggcgttagc ggcgtcacgt
    10741 cacagcagga cgcccaggtc gccaaggccg gcctcgacgt gctggccatc aagtagccga
    10801 aaacggccca tatctcatcc gttgatatcc tgaaagctca agatgtcttc acacgagcct
    10861 tttcattttc atagcctgga agaactgcgc cgccggatcg aggaattgca cctcgacttg
    10921 cctgtcgatg acgatttgag ccctcttctc gagccggtca gatttggtgc agtgccgctg
    10981 ccgaaccgcc tggtcgtgct ccccatggag gggtgcgatg gaaaggacga tggctcgccg
    11041 gacgagctca cttttcgccg gtatcgccgt ttcacggaag gaggtgccgg cctcctctgg
    11101 ttcgaggcct gcgccgtcgt gccggaaggc cgcgccaacc cccgccagtt gtggatccac
    11161 gagaacaacg tcgccgcgtt cgcgaaactg gtcgaggatt ccctgtcggg cgcgcatcac
    11221 gccttcggcc cgagtcaccg tccgtttctc gtgcttcagt tgactcattc gggtcgctac
    11281 agcaagccgg gacgcagccc tcggccgatc atcgcgcacc attcgcgctt tctcgatccg
    11341 atccacaacc tcccgcccga ctatccgctg atcccggacg acgaactcga acgactggag
    11401 gatgtttacg tcgaagcggc ccggttcgcc cgaaaagcgg gattcgatgc cgtcgacgtg
    11461 aaggcttgcc atcgctatct cgtctcggag ttgcatgcct cgttcactcg cgaaggacgc
    11521 tacggcggtt cgttcgaaaa ccggtcgcgt tttttccgca acgtggtagc gaagatacgc
    11581 gacgccgttc ccggactcgt ggtgacctcg cgcatgaacg tctacgacgc gatggcctgg
    11641 ccgtatggat tcggcgtgga ccgcgacgac gcctcgaaac cggatctcgc cgagccgatc
    11701 gaactcgtcc gtcatttggc cgcctccggc tcgccgatcg tcaacatcac catcggcaac
    11761 ccgtatttca atccgtacgt gaaccggcct tttgatctgc cggtggccgg cgcgcccgtg
    11821 cccccggagt cgccgctcga aggcgtcgcg cggtttgtct cgattgtacg gcgcatccag
    11881 atggagttcc cggatatggc cgtcatcggt ggcggctatt cctggcttcg ccacttcttt
    11941 ccgaatgtcg gcgcagcctg cgtccgcaac ggatgggtct cgcttgttgg ggctgggcgg
    12001 atggctttcg cctatccgga ctttgcgcgt gacttgcgaa gtcgcgggtc cctcgatccc
    12061 gagcgcgtat gcgtcgcctg ttcggcttgc acacagatca tgcgcgacgg cggacgcagc
    12121 ggctgcgtgc cgcgcgacgc cgctatctac gagccgatct acaaagcggg ccgtgccgaa
    12181 gcggtcgaca cgatcctcgc catggcgaag acgtgccggc aatgcaacga tccgacttgc
    12241 gtttcgggtt gcccggcgcg cgtgaatatc ccgaggttcg tcggccacat cgccgacggc
    12301 cgcttccgtg aggcgtacga gacgttgcgc gaagccaatg tgctggcagc ggtctgcgga
    12361 tacgtctgcc cttcggaaac gctctgcgaa gccggttgcg tcaacgaaca ctacacggaa
    12421 agcgtgccga tccggcacct ccagcgctgg gtcagccgca aggccgtcga cgagggatgg
    12481 gctgccgaac cccgcccgcg ggagacgtcg accgggaaac gcgtggccgt gctgggagcc
    12541 ggtccggcag gcgtttcggc cgccgcggcg ctcgcctcgc gcggccacaa ggttgtgctg
    12601 ctcgatggga gcccggctcc tggtggaacg gcggcaggca ctattccggc agaccgtttg
    12661 ccagactcaa ttctccgcag ggaaatcgac gacgtcctcg cttccagcgg tgacgtggaa
    12721 cgccgctaca atgtctgctt cgattccaac tatggtctcg acaatgttag aggcggtggc
    12781 tacgacgcat tgctcgtcgc cctcgggctc tccggctcgg tacggctgaa gaccacggtg
    12841 cggccgaagt ccggcgttgt gggtgcgctc gagtttctgg ctgccgtaaa gcgtggcggc
    12901 gcgtcgtccg gagtggtcct ggtcctcgga ggcggcaata ctgccatcga cgccgcgctc
    12961 tcggccaaac gcgctggcgc ggacgacgtg tccatcgtct accgtcgctc atttgccgag
    13021 atgcccgcat ggcccgaaga gcgagatcgc gccatcgaag ccggtgtgaa cttcctcatt
    13081 ctcacgcagc ccgtcggcta cgtcacggat gagcagatga agctcgcggg cttgaaagtc
    13141 gtccgtaccc gcctgagcgt agccggtaag gacggccgcc gcaccgtaga acccgtgccg
    13201 ggttccgagc acagtatgcc tgccgacctc gtcgtggagg ccatcggcca gcaactcggc
    13261 gccgagttga ccgctgccct cgccggaatg cgcttcaccg ccgacggcct catatggacg
    13321 aggagcggct cttttgaaac ctcgcttccc ggcgtctttg ccgccggcga catcatcaac
    13381 ggcggaacca ccgtcgtcca ggccgttggc gagggcgccc gcgccgcacg agagatcgac
    13441 gcctggctcc gcgaacggcc atgaggacac tatttcccac cgcttttgcc tccaccctat
    13501 gtgtgctggc ttccgccctt gcggtttccg cggcggaaac cgtcagcctc tcccgcgctg
    13561 agtacgaaga tcgcgctcgt gctgcgtgga cggcgcagat tctcggcgct ctcatgggct
    13621 tccgcttcga gcacaaaccg gcctcaacgg agtgggtcga caggctgccg aaggcttacg
    13681 atcacgcgcc cgtcgatgac gactactact acgagattgt cgcccttcgc gcgtttgaga
    13741 gatacggcac ggggttgacc gtggaacagc tcggcgctca atggaaggaa aactcggcgg
    13801 gctcgtgggg atctagcgaa caggcccggc tcctgctctc gcgcggcgtg cagccgccag
    13861 ataccggcca tccgcgctac aacaagctct ggttcaccat tggcccgcag ttcagcgggg
    13921 atatctatgg ggtccttgca cccggcttac cgaacttggc cggcaggctc gcgcgggacc
    13981 tgggccacat caacggcttt gccgaaggaa ccgacggcgg ggtgttcgtc gccggcatgg
    14041 tcagcctcgc cttcgcggaa gacaattcga aggagatcgt gcgcaaagcg gcgcgcctca
    14101 ttcatccgca atcgccgtac cggcagtgcc tcgatctcgt cattggcatg gccgaaaaag
    14161 gcgccggccc ccgggagatc gcccaggcgg tcgaggatcg gtggcatatt gaatatcctg
    14221 ctaccaataa cgccgtcgcc aacggcggtc tggtcgccct cagcgtatgg ttcggcgagg
    14281 gagactttct caagaccgtg aacctcgctt tcgcggccgc ggactatacc gacgccgact
    14341 gcaacgccgc caacgcggct gccgtggtcg gagcgatgca cggaatgaag gcgctgcccc
    14401 tggtgctggt ggaagggatc cacgaccgca tccggggcga cgcgatgggc ggtgtgcgcc
    14461 ttactccagc ggtagatgaa gccatttcgg atctcgcccg ccgcaccgtt gccgtaggtg
    14521 agaagatcct gagggctgag ggcgtgccgg ctgacggtga cacgctctcg atccctgcac
    14581 aggacgtcgc cactcagccg gcggaactat tttctctggc ggacctcact cgctattgga
    14641 accccggctg gaagctggag cgtgttggat ttggcggagc aggcggcgga ctcggcggag
    14701 ttcgcggcat tacgtatctc gatggcgatg tgctggcgac atatccacgc gacgaggtgc
    14761 gcggtgcgtt gctccgccgc accgtccgtc tcggtgatca gtcttccctc tccgttcagg
    14821 tggccgccga tcccggccgc gcctggcagc tcgatgcgtt cgccggaaac gagcgtgtcc
    14881 tgagcagact gattgacggc ggccagggta aagagcggga gtggcggcaa atagacgtcg
    14941 atctctcgcg cttcgcaaac cgtgaggtcg aactccgcct cttccagctg gttctcctct
    15001 caggcagcat ccccggcaat gcatattggc gtgccctcgt cgtctcagaa gggctaccgc
    15061 gatccaaata acggagccgg cccgacacga gcacgcacag aggaccaccc ctgacccccg
    15121 gcggcactcc tgcgtgccgg ctcacaccct tctatcgaca gagatggaaa gaacgtgagg
    15181 gttacaatgg cgcgcgtggg ctttctgttc gtcggaatcc tctctggcct cgcgtttgta
    15241 tgctcgctcc cggctcagcc ggtgcggctg gtccggcacg agattgtctc gacacgaacg
    15301 gctgcgatca agggggccgt cctcgatctt cggaaaccgg gtgccccggt tctcttgaca
    15361 tggggacacg gagtcgacgc gcacgcactg ctgaccggac caaaccgcgc catcctcgca
    15421 tcaggtgggg catccttccg agaaggcgga tgcctgttcg acatcgacgg cgacagcgat
    15481 ccggaattgg ttctcctgga ggccggcggc gatctcgtct ggattcggca gaacggttcg
    15541 gtccgcggtg tcatagacac gggcgtcgat tcgcgcgacg tcgttccagc aaccctgcat
    15601 ggccgccgcg gcatcctgtt ggtgcagaag caggcccagg tccgcttcta cgagatcccg
    15661 aagaatgtcc gtgacaagtg gccgtaccgc gagatttact cgttctacac accttcacgg
    15721 caggcgggtc tccgccttga cgatgtcgac ggcgatggcc tgccggacat cctctgcggc
    15781 aattactgga tccgatcccc tgcctcgttc gatctgccgt ggcggctgtt cgccatcaac
    15841 acctggagtg agaccccgga ctcggccatg ttgcggctcg cgctggcgga tgtcgccggg
    15901 cgtggctccc ggcagctaat cgccgcgcag gcggaggtgc cggcagcgcg gctcgcctgg
    15961 ttcgagaagc ctcctgatcc cacgcagctc tgggcggaac atcgtgtcgg aggcgtcgcc
    16021 ggcctccggt ttctgcaagg actggacgcc gccgatttcg acggcgatgg tctcgtcgat
    16081 ctggccgcca gcgaaaacgc cggcaaaaga tcgctcctgt tcctgctcca caacctcggc
    16141 ggaggatcgt tcagcacgca gcgaatcgct tcaaactgcc ccgtcttcta cttgcgcgca
    16201 acagacgtga atggcgacac aaaaccggat ttggtcaccg tcggccctgg cggggccgcc
    16261 tggtgggaga accgaactca cttgcgcaaa taggcgtcgt cgccgcgaat ccagtcctcc
    16321 cgcaccggcg agaacagatc gagggcaacg ctgtcttcga gcgcatcgac gcggtgagga
    16381 gcgtttggtg gaatctggat gacctctccg gccgtcagaa cccgctcttc gcctgcgatg
    16441 gtaaagcgca gtttgccctg ttggagcata ctgatctgct cgttgaagtg gctgtgctca
    16501 ggcacgacac cacccttcga gagatagatc cgtgcaacgg tcatcgtctc cgcatggatc
    16561 acttgccggg cgatctgcgg gttcatctgc tccagcgtca tgttgcccca gtcgtacacc
    16621 ttcatagcgg gtgcattgta tcacggcgga gtggactcaa cgagatacca ggatgtatgc
    16681 tcaactgcac acaggaaacc ctaacccatg cttgactctt tctccatcct ccgaagactc
    16741 atcgtcttga catctctctt cgccctcgcc gccggcgccc agggcggtaa gacccgcggc
    16801 tggtggccgc aaccgtacaa cgtccagcgc gacgatgccg ccggcaagct cgtcctgtca
    16861 acgccctact acaccatcga gcacgacctg aaacagggtg gcgctctctc gaaaatcgcc
    16921 ctgacgcatg ggcgcgcaaa gaacctgctc gcaaggccgg tcgactcgag cgttcagctt
    16981 tcgagccccg aaggccgacc cgcatccgag cgggaagccc ccacggcgaa cacgtacagc
    17041 gaccttttcg acgcctcacc cgacgtctcg cacagcaagg acggcgacac ggaaatcgtc
    17101 acggtgaaag catcgctcgt cgacaagaac ggtgcatcct ccggcgttac cgtctccacc
    17161 acgtaccgct accggtgggg ctacatcaag attcgcaagg agtttcagtt ccccgaaaca
    17221 aagcgcacgc ggaacgtctg tgtgctcaac accgttctcg atccaagcct tacggactac
    17281 ggctaccggc caaacgtccg cgagttgatg gatccggagc tcttcagttg gcgcgccggc
    17341 cagatccggc aatggggcaa gcttcgcgcc ggaacacact tcgatctgcc cgtccaggtc
    17401 cgcatgctgc cgcgctattt tgtcttcgcg aaccccggcg tcgagggcat cgagtggttc
    17461 atgtccgaca acctttatca gtgggactac cagatgacgg gagtccccgg aaccgggtgg
    17521 gcgaacgccc agacttccac cgacccgctc ggcgtcgccg tctctgtgta tccggtaatg
    17581 ctctcctcgc gctacgagct tccgaagggt ggcgctgtga ccctgaaagg ctcctacacg
    17641 ttcgactact acatcgggtt cccggtcctc gaaggtcacg cttacaagcc gtggctcaac
    17701 cgctccttcg cggtcaacaa aggcgagtgg gtcagtgagg aggagatcaa gaagaacgcc
    17761 gcggacggcg tcgtcaccat gcacttgcac aacgacggcg atcaccaccg cgacggcatg
    17821 ttctggcgtg acggcagcta cccgccgtac ccgcccgaag aaatgaagaa aatggacgcg
    17881 gttctcaaga ccatcaagaa gtacggcatc aagaccgcac cctacttctc gaaccacgaa
    17941 ttgcaccaga gcaccgaaga gttcaagaag cacggcgagg agtggggccg gatcgtggaa
    18001 gaccagaata accttcggcc gaatttctac tacggcgctc acatgtgcct gaaatccggt
    18061 tggtacgact tcctgaagtt ctccatcgat cgcgtgctca aaaaccacca ttttgacggc
    18121 acgtactacg actggcacat cgccatgttc tgcaacaacc cgcgtcacgt cggcaagagg
    18181 tcgaacggcg tgtcggatga gaagggcctc ggagcgccgt ccatctctga gaccacccac
    18241 tgggacatcg acgagctgat cggactcgcg gagtggacca gacaacgcgt cggccccgac
    18301 ggcatcatga ttctccacaa cacactggtg ccgatgttcg ccaccgagaa cttcgccaac
    18361 tacatcgtcg gcatggagtt cagctacggc cgcctgtccg tctccgttcc gcagccggcg
    18421 gacgttccgc tcgagtggca gttcgccggt gcgcgcccgc gaaccgtcat cggctacggc
    18481 acgatcgccc gcgaggcccc aaaacgcttg cacagggcac acgcgctcct caccctcatg
    18541 acctcggtcg ccccgtggcc cgccggcgac gaagcgcttg atctcttcaa agccctaaag
    18601 ccgcttggtg acatcgatcg ctatcagttt gaggactatc ggaacgaagc ggtcaaagtc
    18661 gacgaccggg cggtgatgca tgcggtgtac agccgcgcgg gcgaggcgtg ggtgctcctt
    18721 ggcaacgccc agccgcagcc tgctgccgtt cgggtatcgg tcaacgccgc gaaattgcgg
    18781 aacccgctcg cttcggtgaa gaaggccgaa gtcatcgttg ctggtaagac ggccacgctg
    18841 aagccggcga aattgtcagg cgcgggccac aacatcacgg tccccgccga cgacgtcgtt
    18901 ttggtccacc tgaccaacta agctggctca tagaagctca gtgaattgtc cccctgccgc
    18961 agcacacggg tgcggcggag gcgtccgtac atttcttcga gcgcgaagcg tgatgcgtgc
    19021 tgcacgatcg cgagcgccgg cggcgtccag cccaggatct cgagcgcctt accgtactcc
    19081 cgttcctgct cgtacggtgg atcgaggaat acgacgctgg cctcgaagcg ctccagcagg
    19141 gccgtgacgc gtccgctggc aacggtcgcg cgttctgaca caccgagcga atccaggttt
    19201 tcgtgaatca ccctggcggc agctcgattc tgctcgatga acaccgcccg ccgcgctccg
    19261 cggctcagcg cctcgatgcc cacggacccg caaccggcgt aagcatccag gaacacgcat
    19321 ccttcgattc gcggcgcaag tacgctgaac agtgcctcac ggagccggtc cggcgtaggg
    19381 cgtgtccgca tgcctggtaa agatttcagg cggcggcttc ggaattcacc cgcaatcacc
    19441 cgcatcaggc caccatcacc agtccatagc gccgctgcca gtgatcgcgc aggtaggcga
    19501 ccgcacgcag caattcctcc tccgacggcg ggctaccgat gaacgcagtc gcctcggaac
    19561 gcgcaatctc cagaatctcc gcgtcgcgca ggatgttggc gatgcgcaac gccggcagcc
    19621 ccgactgctt cgtcccaaag aattcacccg gaccgcgaag cttcaagtcg atctccgcga
    19681 tgtagaagcc gtccgtggac tcgaccatgg tgcgaatcct ctctttggcc gcgtcgttga
    19741 gtctttcggt gacgagaaca cagtagctct gcgacgcgcc gcggccgacg cgtcctctaa
    19801 gctggtgaag ttgcgcgaga ccgaagcgtt ccgcctgttc gatcaccatg acggtcgcgt
    19861 tcggcacgtc cacgccgacc tcgatgacag tcgtcgaaac cagaatcttg atctcgccct
    19921 ttttgaactt ctccatcacc gcttccttct catctgcggg aagacggccg tggagcaagc
    19981 cgaccgcaag cccgggaaag accacctcgg agaggtgctt gtgcatcttc tgggccgctt
    20041 tcatggcttg cgtctccgac tcctcgatca ccgggtagac gacataggcc tggcgtccgg
    20101 catccacctg ctgcttcagg aagctgtaca ccagctccac tttgtcctcg gtcacgtgtt
    20161 tggtgacgat cggtttgcgg cccggcggca gttcgtcgag gaccgaaacg tcgagctcgc
    20221 cgtagatcgt cagagccagc gtgcgcggga taggggtggc agtcatcacg agcacgtcgg
    20281 gatgcgtgcc cttttccatc aatcgcagcc gctgcatcac cccgaaacga tgctgctcgt
    20341 ctacgaccgc caagccgagc ttcttgaact cgacattctc ctcgagcacc gcatgcgtcc
    20401 cgatgactac gtgcgccaat ccttcggccg ccagtttctt aagctgtgac ttctcccgtg
    20461 ccgtcgccga cccggtcagc agcactacga cgtagccgag cccggagaag agcttcttga
    20521 agtagaggta gtgctgggtc gccaggatct ctgtcggcgc gagtaccgcc acctggtagc
    20581 cgttttcgat ggccacaatc gccgcctcgg ccgcgacgac ggtcttgccg ctcccgacgt
    20641 cgccctggag aagacggttc atcggatgcg gttccgccat gtcgcgagcg atctcgccga
    20701 gcaccctctt ctgcgcggcc gtcggccgga atggtagcat gcgcttgatc ttctgccggg
    20761 cgttctcgga tagctcgaac ccgattccgg tccttttgcg ggctttgctc cgcttgagcg
    20821 ccagcccgca ctccagccag aagaactcct cgaagatcaa tcggaactgc gccggcgacc
    20881 ggaacgcatt gagcaggcgg aggtcttcgc ccgccgctgg gaagtggatt tgttgcaccg
    20941 ccgtccgccg gtccatcagc ttcaaccggg cgcggacggc cggaggcaac ggctcatcaa
    21001 gcgcgggcag gttctcgaga gccttgtaga tcagcttccg gaagatgcgt gtggtaatct
    21061 tgcccgcggc ctcgtaaatc ggcacgattc gcccgacatg gagcgccgcc tctccttctt
    21121 cttcatcctc cgtcagaatt tcgaactcag gatggagaat cccgagccgc ccgctgtagc
    21181 tgtcgtactc gatcttgccg tagagagcga tcttctgccc tggcgcgatg acgtccttca
    21241 gatatccgcc gtgaaaccac ttccccatga ggatttcgcg ggaggagtcg gtgaaactgg
    21301 cctcgaacag cccaaggttt cgccgttgga aaccggagag cttcgtcgtc cggaccgtgg
    21361 cgaccaccgt ggccatctca cccggtgcaa gctgtacgat cgttttggtg ttgctgcggt
    21421 cttcgtaccg gaagggcgca tacccgacga ggtcctccac cgtcaggatg cccttggttt
    21481 cgagcatcgc cgcgcgcgcc ggtccgacgc cttttacgta ctggactggg gtagagagat
    21541 ccacggccgg caaccgcata ctgacgttcg tgacttggcc gcctgtcgtt tccgagccgc
    21601 gaccggagcg gtcagcccca ctttcgctta agcctcggtc caatccggtc gaggcttgcc
    21661 tcgccctagt gtacaatgcg cgtgatgaaa gctctcgtac ttctctccac gctctctgct
    21721 gccgtctcga tggcgcagat ccgcgtcggc atcatcggca ccgacacgtc gcatgtccct
    21781 gccttcacca aagttctgaa cgacccaagt tcgccggacc acatccccgg cgcccgcgtg
    21841 gtggcggcgt ataagggcgg cagtcccgac gtcgagtcga gctacacccg agtcgataag
    21901 ttcgccgagg agatccgcac aaagtggaac gtcgaattgg tcaaagacat cccgacgctg
    21961 tgctcgaagg tggacgttgt gctgctcgag agcgtcgacg gccgtgtgca cctggagcag
    22021 gtgaaaccgg tgatcgccgc agggagaccc gtgttcattg acaagccgct cgcagcaacc
    22081 ctcgatgagg ctcgcgagat cgcgcggctg gcgaaacagg cgggcgttcc gtggttcagc
    22141 acgtcgagcc tccgctacag cgaagtcgcc acctcgatga agtatcccga cgccaagggc
    22201 gtgatcacgt ggggacccgg cccgaccgag ccccatcact acctcgacct ctcctggtac
    22261 ggcatccacg ccgtcgagat cctctacaca ctaatgggcc ctggatgcga ggaggtttcc
    22321 cgtacgtcct cgccggacgg cgacgtcatc gtcggccgct ggaaggacgg gcgcaccggc
    22381 acagttcatt cgctgcgcac ttcgagcgag tacggtgcgg tcgtgttccg tccgaagcag
    22441 atcgagcaga gccgacccga catgaagtcc ggctatgcgc cgatgctgcg ggagatcgtg
    22501 aaattcttcc agacaaagca accgcccgtg ccgaacgagg agactctcga gctcttcgcc
    22561 tttatggatg cggcccagcg cagcaaggaa gcgggcggca aaccggtgcg gctgcggtaa
    22621 cggccagcca cgggagcaag gagagctctt ctgatgacca gacgggaatt cggtgagtgg
    22681 atgatggcgt cgggcgtgct ggccagcacc cccctaaagg cccaggagac ggtggaagcg
    22741 acgggatccc acatcggcaa cctctatccg ttcgttcaga aacaggcgga ccagtcgaag
    22801 ctcgagcttt ccttcctgcg gccggagttt cgcaacctga aacagtggaa gtcgcgggcg
    22861 cgggcgcgcg ttttcgagca gatgttctac tcgccgcccc cggtggaccc cgtgccgcgc
    22921 cagatccgcc gcacggaccg cggcgactac atcgaggaat atctcaactt ccagaccacg
    22981 cccgatatcc gcgttccggc cttcgtgctc gtgccgaaga acgcgaagct cccggcgccg
    23041 ggcatcgtcg cgctgcacga ccacggcggc ttttaccttt ggggtaagga aaaactcgtc
    23101 ccgcaggacg gcgagcaccc ggcactgacc gaattccgga agcgttacta cggcgggcgg
    23161 agtgtccccg tcgagctcgt ccggcagggc tacgtagtca tcgtcattga catgttctac
    23221 tggggcgagc gccggatgct cctcgacgac gatccgcccg acttccgtga gcgtccctcg
    23281 tcaatgcccc aggagcggat caacgagttc aatcgccgct ccggccagaa tgaacaactt
    23341 gtggcgcgga gcctgttcac cgccggcata agctggcctg gcgtgctcct ctgggacgat
    23401 atccgcacgc tggactacct cgcctcacgg cccgaggtgg acaccaaccg cctcggctgc
    23461 gtcggtcttt ccgtcggcgg ctaccgcagc ttcctgcttg ccgccctcga tgagcggatc
    23521 aaagcggccg tggacgtggg ctggatgacc tccttcgcct cccagatccg aacccaggta
    23581 atccactccg taggcctcac gtttcatctc gtcgggcttt accggtatct cgaccttccc
    23641 gacctggccg ccctgatcgc gccccggtcc ctgatggtcg tcaacggctc gaaggacacc
    23701 ctgttcgcgc ccggcggagt gaaggcggcg ttcgagaaga tcgaccgaat ctaccggaaa
    23761 gccggcgctc ccgaccgcca gcgctgccgc cttttcgacg tgcctcacga attcaacctc
    23821 gagatgcagg ccgaggcctt cgactggctc aagcgttggg tgtaggatcg cttacggtac
    23881 aataactggt tcatgtccaa cctgttggag ggcaaaactg cccttgtcct gggagtcgcc
    23941 aacaagtgga gccttgcgta ctcgatcgcc gaggcgttcc gccgggaggg tgcccgcgtc
    24001 ttgctcacct accagggtga gcgccagcga gcgaccgtcg aggcgcttgg ggcggaactc
    24061 ggtgccgccc gcatgctcgc ctgcgatgtc acccaggacc aggaactgga acggcttatt
    24121 gagaccctgc gcgccgagga cggccggctt gatgctgtcg tccatagcat cgccttcgcc
    24181 aacaaggagg atctgacacg tccgttcgtc gagacctcgc gctccggttt tctgttggcc
    24241 catgaggtga gcgcctactc gctggtttcg gtggcccgtg caaccgcccc actcatggcc
    24301 ggcggcggct ccctcattac gcttacctac ctcggcgcaa cccggataat caccaattac
    24361 aacgtgatgg gcgtcgccaa ggcggcgctg gaatcggcga tgcggtacct ggcaggcgag
    24421 ctcggcgcct ccggcattcg cgtgaacgct atctccgccg gcccgatcaa gaccgcctcg
    24481 gcccgggcca tcaaggattt ctcctcggtg ctcgatttcg tcgcacaaag ggcgccccta
    24541 cggcgcaaca ccgaaccggc tgaggtcgcc gacgccgccg tcttcctcgc cagtgacctc
    24601 gggcggggcg tcaccggcaa catcctgttt gtggacgcgg gattccagat cgttggtctg
    24661 tagcttgaga gtgcattgac agtgcttttt caccgtcttt atgatgaaag ttcagctacg
    24721 ggagttcgtt gatgcggcac tcccggaaac gcccgacaac ttccggaaat caatatgatc
    24781 aaagtcgaag gtctcacgaa gcgatacgct cgtaacgtcg ccgttgacaa tatctcgttc
    24841 gaggtcgaca aaggcagcat cgtcggcttc ctcggcccga acggcgcagg caagacgacg
    24901 acgatgcgcg ttctgacctg ctttcttccc ccgaccgccg gtggcgcaga ggtcgccggc
    24961 ttcgacgtga tgaaacagcc gctcgaagtg aagaagcgca tcggctatct gcccgaggcg
    25021 ccgccactgt accccgaaat ggaggtcgtc gagtacctca acttcgtcgc caacctcaaa
    25081 ggtattccgc gaggagagcg aaagcagcgg gttgacagcg tcaccgagcg ctgcgcgatc
    25141 ggggacgttt cgacaaagct gatcggcaag ctctccaagg gctaccgcca gcgcgtcggg
    25201 ctcgcacagg cgatcatcca caaccctgac gttctggtgc tcgacgagcc caccgccggc
    25261 ctcgacccca aacagatcct tgagacccgc gacttgatca agcagctcgc cggcgaccac
    25321 accatcatcc tgagtaccca cattctgccc gaggtggaac agacctgcga gaaagtgatc
    25381 atcatcagca agggcaggct ggttgccacc gactccgtgc agaacctcac cagcaggctg
    25441 cgcggcgccg aagccatcgc cgtggaggtg gagccgcgag acactgcccc gctcgacacc
    25501 gttcaggtgc gccagcggct ggagcacgtt ccgggcgtga gccgcgtcgt cgccaaagag
    25561 agcaaggaaa accggtgcat cttcgaggtg gagagcctcc aggggcgcca catccgagcc
    25621 gacctggccc gggcggtcgt ggaggccgac tggaacctga acgagctgcg gccggtggcg
    25681 tttagtctcg aggaaatctt ccttcagttg acatcggccg accgggcagc cgagggaggt
    25741 gagaagtgag aaacgtctgg accatctatc gcaaagaact caagagctat ttcgcgtcac
    25801 cgatcgccta cctgctgatg gcggtattcg cgctgatctt cggctacttc ttctatgtcg
    25861 cgactgccat cttcgttagc cgcggcatgg agtcccagat gatgggccgt ggatttccca
    25921 tggacgtcaa cgaatgggtg atccgcccgc tcatgatgaa cgtcagcgtc atcggcctct
    25981 tcatgatccc gatgatcacc atgcggctct tcgccgaaga gaaacgttcc ggcacaatcg
    26041 agttgctcat gacttcgccc atccgagacg ttgaaataat cctcggcaag tggctggcag
    26101 cgctcactct gtacgcctcg attctcggac tctcgatgat caacgtcgcg gtcctgttcc
    26161 tgtatggcaa gcccgactgg aagccgatcc tcgtcggcta tctcgggctg ctgctgcaag
    26221 gcggatgcct gctggccgtg ggaaccttca tctccacgct gacgcgtaac cagatcatcg
    26281 ccgccggcgc caccttcgcc gcctgcctgc tgctctggat tttggactgg gtcagcgcct
    26341 atgaaaccgc cgcttgggcc aaagtcttgt cctacatgtc ggtggtgacc cacttcgagc
    26401 cgttctcaaa aggagtgctg gacaccaagg acgtgatctt ctatctgtct gtaatgttcc
    26461 tcggcctgtt tctcaccgcg cggtcgatgg aatctctgcg ttggagggcg taaatgacat
    26521 tcgactggct gaaagcacga caaacgaaat acaccgctta cctcacgctc tacctcgcgg
    26581 tgatccttgc agtcctgggg gcggccaact ggctcgccaa ccgccacaac aagtccttcg
    26641 attcaacggc gaacaaacgg ttcagcctct cggaccagac cgagaaggtc gtcaagaatt
    26701 tgaagcagga cgttaagatc gtctacttcg accgcacctc cgacttcacc cgcgcccgcg
    26761 acctcctcga cagatacgac aacctctccg ccaaactgac cgtcgcctac gtggaccccg
    26821 accggaagcc gcagatcgca aaatccgagg gcgtgcgtaa ctacggcacc atcttcgtcg
    26881 agaccgccgg caagcgggag gaggcgaaga gcgtcagcga ggaggaaatc accggcgcac
    26941 tcatccgcgc cctgaagggc ggcgcgcgca acgtttgtgt cgtctccggc agcggcgagc
    27001 acagcctcga cgacaccgaa cgcagcggct actctggtgt gaaagagctg ctcgaaaaga
    27061 acaactacaa aacccgcatc gtttcgctgc tcgaaaaacc cgaagtcccg aaggactgta
    27121 ccatcctgat ggtcggcggt ccgcggtttg actacgtgga cgcctcggtc aacgccatcc
    27181 gcgccttcgt cgaaaacggc ggccgcgttc tgctgatgat cgacccgccg ctgaagatgg
    27241 gaaaagagga catcgcagat aatgccgctc tcttgaaggt gctggaaagc tggggcgtaa
    27301 cgccgaacaa ggaccttgta ctcgacacga gcggcatcgg acagctcttc ggcttgagtg
    27361 aagtcgttcc gctggtgacc agctatgagt ctcaccaaat cgtccgggag atgaaggaga
    27421 tcgcgaccgc gtttccgatt gctcgctcac tggaggtgaa gtccggtgac aaaaccaccg
    27481 tcgagaagct gttctccaca tccggcaaca gctatgccac ctcgagcctg agttccagcg
    27541 aaatccgcat caatccggcc aaagacaaga agggccccct caccctcgcc gcggcgggca
    27601 cgtaccgcac cggaaaagag ggcggcgacg gccgtttcgt ggtggtcggt tcgtcgggct
    27661 gggcagccaa taacatcctg cgattcaacg gcaaccgcga cttgttagtc aacatgatga
    27721 attggctctc ctcggacgag gacctcatct ccattcgtcc caaggaaccg gacgaccgcc
    27781 gattgactct tacccgtcgg caaatgtcga cggtatttta ctcgagcgtc gtcgggctgc
    27841 ctctcgcggt ggtgctggca gggatgtggg tctggtggcg gaggcggtaa acgatgcgaa
    27901 ttcgcgggct gctcatcgct gttgtggtgc tggcggcgct ggccggcggc gtctactggt
    27961 cgaacaaagt gaagaaggcg gaggagggca agccttctcc tgatgcgccg ccgaaggttc
    28021 tctccattcc gcaggaccag ttccagcaga tcgagattca taagggcggc gtggatgccc
    28081 tgacactacg taaagacgat gccgggaagt ggcaaatggt ctcaccgaaa cagttgcccg
    28141 tggaccagga tgccgcgagc agcctcgtct cgagcctgtc ctcccttgac tcctcacggc
    28201 tggtcgagga aaagtccgcc gatctcgcgc aattcggact ggcgagtccg tctctggaag
    28261 tgaacgtccg tcaaaaagac ggcaaggtga ccaaacttct cgtcggcgat gaaagccccg
    28321 ccgggggtgg ccactttgtc aaactggacg gtgatccccg cgtctttacg atcgcctcct
    28381 acaacaagac cagcatcgac aagacccccg ccgacctgcg tgacaagcgc ctgatgacct
    28441 tcgactccga caagctcaca cgagtcgagc tcaaggcaaa aggtcagtcg atcgagttcg
    28501 gcaagaacaa ccagaacgag tggcagatca tcaagccgaa gcctttgcgg gccgacggcg
    28561 gacaggtgga ggaactcgtc cggaagctga aagacgcgcg gatggaaacc accgtcagtg
    28621 atgcggacac aaagaaggcc gctgccgcgt ttccaggcgc caaaccggtc gccgttgtcc
    28681 gcacgacgga tgccgccggc acgcaggaac tcgacgtccg caaggacaag gataacaact
    28741 actacgcgaa aacctccgtc gtcgagggcg tccagaagct gacaaccttc gtcggcgaag
    28801 ggctcgacaa aggcctcgac gatttccgta acaagaagct gttcgatttc ggcttccacg
    28861 atccgagcaa gctggaaatc cgcgatggtg gcaagcaggt cgtctatcag aagacgggcg
    28921 acaagtggat gtccggctcg aagcagatgg atggcccaag cgtccaggcg ctcgtggata
    28981 agctgcgcga cctctcggca atcaagtttc tcgaacaggg atccggcagc accgtcttcg
    29041 aagcggccgt gacctcgaac gaaggtaagc ggttggaaaa ggtgattgtc tcccggcagg
    29101 gtgacggctt cctcgcccgc cgcgagaacg agcctgcggt gtacgaacta gacggcaagg
    29161 cggtcgagga gttgcagaag gccgccgcgg cagtcaagga gcttcagccg cccaagaagg
    29221 aagaaaagaa gaagtgaccc ggtgaaagca atagacggtc ttctcaccga tctgtacgag
    29281 ttgacgatgt cggccggcta ttttcaggcc ggcaagtcgg aagagaaggc cacgttcgag
    29341 ctgaccgtcc ggaggctgcc gaagaagcgg aatttcgtca tcgtcgccgg cctcgagcaa
    29401 gttgttgagt acctgctcga gcttcacatc accggcggcg agatcgacta cctccgccgc
    29461 cttccccagt tcgccaatgc gccccaaggt tttttcgagc ggttgcgcga tctgcgcttc
    29521 accggggatc tgttcgccgc ccccgaggga accgtcttgt tcgccggcga accggtgctg
    29581 aacgtccgcg ctccgctgat cgaagcccag atccccgaaa cgtacctgct gtcgatgatc
    29641 ggctaccagt cgatggtggc taccaaggcc gcccgcattg tagaaaccgc ccgtgggcgc
    29701 ggtgtggttg agttcggtac ccggcgggcg cactcgcccg aagcgggtgt cctcggcgga
    29761 cgagccgcct acatcggcgg ctgcgtcggc acaagcaaca cgctttctgg ataccggttt
    29821 ggcatccctg tctatggaac ggccgctcac tcttgggtgc aatcgttcgt cgacgaatcg
    29881 gagtctttcc gcgagcttca gaaactgctc ggcgctttca ctatctatat cgtggacacg
    29941 tacgacacgc tcgaaggcac ccgccgcgcg gcggctctgg gaaaaccgat ccagggcatc
    30001 cgtctcgaca gcggcgatct cggcgcgctt gcgcgccagg ctcgggtcat cctcgacgaa
    30061 gcaggtctgc ccgacgcgaa aatcatggcc agcggggact tggacgaata caagatccgc
    30121 aagctggtca atgagggtgc gccgatcgac tccttcggcg tcggcacgga actggtcacg
    30181 tccgccgacg cacccaacct cggggtcgtc tacaaaatgg tggaggtcga gaacggcggc
    30241 gtcaagcgct acacggccaa gttcagcgag gacaaagtga cacttcccgg cgcaaaacag
    30301 atattccgtt acccggaccg tgacgtgatc gggcgagcat cggagtgccc gccgtctgcg
    30361 agtcaggtgg aggcgctcgt gcgtccggtg attctcggtg ggagcctggt cgagccgctg
    30421 cccggcgcgg ctgccgtccg cgagcgtgcc gctgaatccc tccagcggct gcccaaagcg
    30481 tgccggcgcc ttcgcgctcc caaaccgtac cacatcgagc acagcgcgaa actgctcgcg
    30541 ctcgcggagc agtcccgcaa aggcctgacg gtctaggctg gagaagtgcc catgaccacg
    30601 gtattctttg acatcgacac ccagaacgac ttcatgcttc cgtcaggagc gctatacgtg
    30661 ccgggcgccg aggcgatcct gcccatgatc gcgcgcttga accgctatgc cgtgcaccac
    30721 ggcatcattg tcgtctccac caccgacgcg cacgatgaga acgatccgga gttccggcag
    30781 tggcctccgc actgtgtggc cggaacgatc gggcagcaga aacccgccga aaccttgctc
    30841 gagcgccgcg ccgtcatccc gacgacgccc ggagtctacg aaattgacgg cgcacagcag
    30901 atcatcgtcc agaagcaggc gctcgacctc ttcaccaatc ccaacctgcc tgcgctgctg
    30961 gagcgcgcct cccgttacgt ggtctacggg gttgtgacgg agtactgcgt tcgctgtgcc
    31021 gtgctcgggc tgcttaagac cggaaagccg gtggaaatgg tgaccgacgc gatccgaact
    31081 ctgaaggatg acgacggtcg ccggacgatc gaagagttca ccgctgccgg cggcgtcctc
    31141 acgaccgccg aacgcgtctg cgcgggttga gagtggctgt cgagcttcag cggtggcaca
    31201 ggcttcggcc tttgatggtc gatgctctca gcgggttttt cagcaccctg tccaggctgt
    31261 ggagggattc acccttccgg caattccacc ggcttggcgc tgcgccacag ccgctcgaga
    31321 tcgtagtaga cgcgggcctt ctctgagaag acgtgaacaa tgaaatcgcc gtagtcggca
    31381 agcacccagt cggcgttgtc gtacccttcc agactcatag gcagttcgcc ccgccgcttc
    31441 agttgaatgt tgatctcgtc gctgatcgcc tggatctggc gcgggttggt cccggtacag
    31501 atcacgaaga aatcggtgaa ggaggtgatc tcgtgaaggt ccagtacccg gatgtcaacg
    31561 gctttcttcg attctgcggc gcggaccgcc gccagccaca aagggctcgc caattcggag
    31621 ttttcgtctt cgggcttccc agtctcgtgc gcctgcttgt cggatctcac tctgctcagg
    31681 atctgctcct gccgagtatc gtactacaat cgggaattcg attgatttgc catgcctcag
    31741 ccttcgtttc gagtgctgat tactgccgaa cggattcagg agagaatctg cgagcttggc
    31801 gcccggattg atgcggacta ccctgaggga cccgtctacc tcgtcgccat cctcaagggt
    31861 gcatgtttct tcctctccga ccttgcccgc gccatcaccc gccctgcgcg gatcgacttc
    31921 atcggcattt cgagctacgc aaagggaaag accagctccg gcgaagtcaa gctcaccaag
    31981 gacctcgact cgtcgatcga gggcgcccac gtgctgatcg tcgaggacat tgtcgactct
    32041 gggattacct tgaactatct gctgaaggta ctgtcgcagc gcaagccgcg cagcctccgc
    32101 atcgccgcac ttctcgacaa gccctcacgg cgaatccagc ccgtcgacgt cgcctattcg
    32161 ggcttccaga tccccgacga gttcgtcgtc ggctacggcc tggactacgc cgaggactac
    32221 cgcaacctca aggacatctg cgtgatcgag tagtcgcctt ttctttgtgc ctttgtgcct
    32281 tcgtggtttg cagtccggct gcgaccctac ctctccccgt ctcagctctt ctgcggcttc
    32341 ggcgtcttgt ccgtgctcgg cggcaagacc ggctccttga tgtaatcgcc cggcacttct
    32401 ccagtgagcg tcttcagcca ggccacgatg gacgcgatct cttcctgcgt catttgtttg
    32461 ccgagttggt actcggccat atggctgacc gcttcgtcga gcgtctttat tgagccgtcg
    32521 tggtagtagg ggccggtcct ctcgatgccc cgcagcgagg gcaccttgaa catcatcttg
    32581 tcgccttcct gtttggtgac cttgaaccgt ccctcgtcgc tcgtgtcagg ccacggcttc
    32641 accacgccga tcttctggta catctgcgca cccacatacg ggcctgcgtg gcaagcctgg
    32701 cagccggcct ccatgtattt gttgaagccg gccttctcca cgtcggtcag cgcggtctgg
    32761 tcaccctgaa gaaacttgtc ccatcgcgac ggcgcgacca gtttcctctc gaaggcgccg
    32821 atcgccttcg ccatgttgtc gaacgtgacc gggtctttgt cgtccgggaa ggctctcttg
    32881 aaggcttcca cgtatcccgg catcgatttc agcaccgcaa ccacctgctt gttggaggtc
    32941 atggccatct ctaccggatt catcaccggc cccttcgcct gttcttcgac gtccggagct
    33001 cggccgtccc agaattggac gaagtggccg gcggcgttga agacggtggg agagttcctg
    33061 tcgccccgct tgccgaggtg accctcggac gtctcctcgc catcgacgcc gaaattgtca
    33121 agcttgtggc atgtgttgca ggatacgtcc tgccccttgg acagtcgctt gtcgtaatac
    33181 aacatgcggc cgagggagat cttttcctcc gtgagagggt tggaggccga ctccatgacc
    33241 gcgggcagcg gcttgaacat cgcgagtttc gcggtgtcaa cctcgacttt tttcggggcg
    33301 ctcgtgcagg cggcgagcag agcgaacgcc gcgcacgccg ccaggatctt ccagaggagc
    33361 gtgtgtttca ttgcgtgttc tcctttttcg aaggcggttt tgacaaaccg gaccgaaata
    33421 tccttatatc acgcccggag cactcgcacc gggcagaatc ttgggcgccc tcgcaaacgt
    33481 gagtcgtggc gattgagtgc aggaccaggc ttccggcttg actgccgaga cgcaccattt
    33541 tggtgcatac tgatgatatg gggtccgtaa acaagccggt ccggcagagc gtcaccttgc
    33601 catccaaggt tgccgtacag gttcgccgga tggccagaaa tcgccggctc agcgctaatc
    33661 ggatgttggt ggaactcatc gagaacggca tcgaagcaga gaagcaaaaa caacgggaat
    33721 tcttcgagct tgccgagcgt ttccgcgatg ccaccgatcc cgatgaggtc aagcgccttg
    33781 gcgacaaact cggccgtctg gtatttggtg actgatgccg aagatggaga gttgggacaa
    33841 gtttcccgcc gcggtccggc aacacttgat cgagcgaatg cgtgacaggg agatcagtgt
    33901 ctcggacctg aaccagctcc gtctctgggt cgaatcaaag ccggaggtgc cggagggtga
    33961 ctggtacaag gacttcggct cattcaaggt ctgcggaaaa ggctcgtttc cgaaaacgtt
    34021 tctcctccgt ggtcaacccg ccaaatgtct ggccctctga gtggcgcccc gatcggccag
    34081 gccccctact tcttgctggg gttaatcatg atgtgcgcgc ccgcggtccc ggggtacatc
    34141 agccatggcg cgttctcggc gggcttggtc gacagtcccg tcgactccgg cgtggcgaag
    34201 ggcacgtaga tcacccagcg cagataggcg tcggtcacct tcccggtggc ggcatcgaag
    34261 ccggttccgg tgagtacgta aagcgtccgc ggctcgcgcg gcatggcgag tttcccctcg
    34321 gcaacctcct tccagcggac ctcgttgcgc tctttcccac cgatcttctg ggcagccagc
    34381 tcgcgcccgc gcgccatgta aggttcaaga tcgcggtggt aacaggcggc gctgaacgac
    34441 ttctggcccg ggtcgctggc aagacagatg agatcgttct tgccctcgcg gagcgtgaca
    34501 agcgccccct gcgcgttgta gccgagaacg gccgctccag agcgaagttc ctccggcgcg
    34561 gcaaggacgg cggaagcgat ctgctccgct ggggtgggca cgtccgaggc agcacacagc
    34621 ccaaccgcca ggagcccgga ccaaagcagc atttgggtcg ttttcacatc gaatcctttc
    34681 cccgaaaacc cttatacgcc gctcgcgagg ggaggatagc cacagaaccg cgctccccat
    34741 tgcccgcggt cgtcgttagc agctatttta cagcggaagg aatgaccgct ttgcgaatga
    34801 tgttagactc atgctcatcg gaaatgtctg gtatcagcgc ttcaggccgc cggattggtg
    34861 tcgttttgtt tcaactcgga ggtccggatt ccgtcgacgc cgtcgagcca tttctttaca
    34921 acctgtttag cgacccggat attatcgact tcccgttggc ctcgatcgcc cggcggcccc
    34981 tggcgcgtat gatcgcccga gcccgcgcgc atcacgtaag tcgccattat gcggaaatcg
    35041 gaggccgttc gccgattctc gaagtgacgc tgcggcaggc cgctttgctg gaacgcgaac
    35101 tgcggcgcag tctcgacgca cgcgtggtgg tcgcgatgcg ttactggcat ccgctcacag
    35161 gcgaagcgat ggaggaactc cgaaagcacg cgcccgatga agtggtgttg ctgcccctat
    35221 acccgcaata ctcgcgaacc accacgggca gcagtctgaa tgaatggaac cgcctttcgc
    35281 gtctgtcgga cacttggcgt ccaaaggtac atctggtccg cgaataccac gccgatccgg
    35341 atttcatcga tgctctcgtc gcagcggtga acgagtccct ggcaggattc ccgcacccga
    35401 tcacggaact ggacttcctg ttcagtgctc acagcctgcc cctctccgtt gtccaggcgg
    35461 gtgatccgta ccaggagcac gttgagcgca cctgcgatct ggtatgggcg cggggaggtt
    35521 ggccgggcag gcgccacctc tgctaccaga gcaaggtggg cacttccaag tggctgggac
    35581 catcaatgga agaagccatt gagcgcctgg cggcggaggg cgcccgtcat gtcctggttg
    35641 tccccatttc attcgtttcg gaccacgtgg aaaccctgta cgagatcggc atcgaacacc
    35701 gggaactggc cgagcgttta ggcatcgcgg atttccgcct tatggccggc ctcaactgct
    35761 cgccgcactt catcgccgcc ttggcaaagt tggtgcggca gcgcctggcc gtcagcgtct
    35821 aagcagtttc cgaaccgtga cggaaaggag cggtcagtct ctttcggccc gcggctcggc
    35881 tgatacgcaa ctaaggggtt gcatattgca gggcacttgg gatattatgc aacctggagg
    35941 ttgcgcaatg acgcttacgg acttgagccg gattgatcgg gcgatccaga tccaggcgcc
    36001 acccgaccgc gtttggcgtg cgctcaccga cgcccgggag ctatcggctt ggtttcaggt
    36061 gaccatcgag ggcagcatgg agccaggcaa cgaagtgtgg atgacctctg tccacccgga
    36121 gcaccagggt cagcgttttc gtgtgtggat tgtggagatg acgccggccc gccgggtcgt
    36181 ctggcattgg catccgggcg aggtggatcc cagtgtggac tactcccgcg agccgcgaac
    36241 caccgtcact ttcacgctgg agccggcagc cggcggaaca cggctcagta tctcagagac
    36301 gggtttcaac gaaatctccc tcgcgcgccg ggcaaaggtc tatggcgaca acgcccaggg
    36361 ctggacggaa gtggtcgtct ggctgcaaaa gtatgccgaa acgggtggtt gagcggaatc
    36421 tggcgggcgt cgcggtcatg ttcgcggcac tcggtgatga gacgcgcctg ctgttgctga
    36481 ggcggttgtc gcaaggtggg ccggcctcga tctcggtgct ggcggatcgc ttccgcctga
    36541 ctcgccaggg agtgacaaag caccttcatg ttctcgcgga cgctggaatc atcgacggtc
    36601 accgcgcggg ccgggagcat atttgggcac tgaatccgac gcgactcgcg gaagcccgcc
    36661 gtcacctcga cgtgatcgcc cgcggttggg acgacgctct cacccgcctg aaaacgctta
    36721 tcgagggcga ctagccccca ccattccgga ttcgccctcg gtagcgtggc tctttgccgg
    36781 cgctggccac gcttgagggc gtcccccatt tcggcctcct cgcgctgttc ctcaccggcg
    36841 cgccgagagg ctcgacactg ccttgtggga agatccggca agcactttcg atgggtcgtt
    36901 ggcattccgt gagttggagc tcatccgatc ggcggccaac tgccggtttg ctcaatggcg
    36961 cgccgcagga tcagtaaacg ccaggcgaca aggcaggatg attgaatggg ctgtcgtgac
    37021 ggctggtccg tccggtcttt acgcaacgac cgattgcgca ccagaagaca gtcgccggaa
    37081 agaggtgaag acgcgcccac tgcaaatcga aaagagtttg agacgccagc tcagccagcc
    37141 gtcccaaaag agcgcatctg agacgctccg ccaacgatcg tggccggcga tgtcggcgcg
    37201 cttccgggca attatcgacc gaaaacggta gccgcgatcc aggtgcctat tttgtccagg
    37261 gctgatgggg aaatcgtctc ttcgatctgc ccgtattcgc ggaaagcgcc agttttggcg
    37321 gtctggagga gatgattcag gccgggtatt tcaacggctt caaagtgctt gttgccccca
    37381 tcttgcaagg cttcgcggat cgccggcaga ttcagcttgg gagggacctg agtatccttc
    37441 tctccgttga tcgccagtac cggacaggag agtctgcgca gggccggagc tggatcatag
    37501 gacaggaaat agcggtacca cggcgaggtc atctgactat aagcaacgtc ggcctgccgc
    37561 cccaaaagat ccttgaactt ctgtttcagg acggcttcat ccttctcctt catcagcagg
    37621 tcgagaatct cacgctcttt gaccccggcc tggacagcct gttcgtgcgg cattccggcg
    37681 gcttctccgc ccgccaccgt ttgagctacg atgatctggt cgcccggaac gccgggaccg
    37741 gccatgagaa cgacaaaggc tacgtctttg ctacgtgcgg ccaccatcgg cgcgatgacg
    37801 ccgccttcgc tgtggccgat caagccgatc ttgtgtggat caatctcgcg gcgggtcttg
    37861 aggtaagcca gagcggcttc ggtgtcggtg gcgaagtcgg cggtggtggc ggtggcgaag
    37921 acgccgccgg attttccggc gccgcgatcg tcggaacgaa gcacggcgat gcccttgcga
    37981 gtcaggaagt cagccagcac caggaagggc ttgtgcccga gcaggctttc atcgcgatcc
    38041 tgctggcccg acccggtgat cagaatgacc gcaggaaacg ggcctggcct gtttggtatc
    38101 gtcagggttg cgccgagctt gatgccggcg gtgcggttct cgtaaacgag gtcttcttcg
    38161 cggtagggat agggttgggt ggggttctgc ggccgccgag gctcggccag aacttccgtg
    38221 ccggctcgtt tgaaattcaa gggaaaagat tcgcgttgcg taaacgtgcc ctcaatggcg
    38281 gtaaagtcct tgttcaccgt gccttcgtag tggattgccg ccaacgcgaa ggtcagcttg
    38341 gactctttga gttctatgga acggattggg atccctttgg cattctgatc gaggctgtcc
    38401 atagtgcctg tgaggatgcc atccgttccc ctggtaatgt gcacgagaaa ccgcaaatcc
    38461 atgggtccgg catgcagagt gccgagccag tcgccggcga tgtcctgcgc cagcacggga
    38521 agagccgcta gaagcgtgag aaagaaaggt cgtttcatga tagtagatct cctgaagtcc
    38581 gcgcggtttc aaagcctccc cgcgccgctc cagtttcaag gcgcgcgctc gtttatgacc
    38641 gcaacgcgcg ggccagcacg acgattccca ccggaaacag caggaatgcg gccacaacaa
    38701 tccacgccag ccggttgccg aagttcaaag tgagtccgag gccgatgcgc ttttggaccc
    38761 atagcgctgg atcttcgggg tcgtagtaga acaggtcgcg atgccagcag gcatccggcg
    38821 tgacttccgc tcccggctcc ggggcgggca cgcctcgccg gaagtgacgg gtgaacacca
    38881 cggccaggaa gacaaagaac gcgccattca atgcaaccgt cgcccaagtg ggagcaacgc
    38941 gaagtggcag aagaccggcg atcgagaatg ccgcaccgac cagccagccg accgcgatga
    39001 gaatgatccg aaccactgga tgcacggcag ctctacggga accccaggtg atgacggtgt
    39061 cgatcgccac gataaacacg atgagcagcg cgcccatcaa cagtgggccg aacacaccca
    39121 agggcgtccg ggtggaccag gcgttcggca ctccgttttg gccccagtgg atcgggaaac
    39181 gggcgggaat ctcgtcccaa cgaaaatgca ggtaaaccgc ggtggcagcc aacagagcca
    39241 gcgggagaag aaaccagggc agtcgccggg ccaggcctga atccgcgtgc aagcgcgcct
    39301 cgcgggtaga gggagctggc agcgcaagcc cacgtagctc ggcgcggccg gcggagaacc
    39361 cacggtaggc cgcaatgatt ggcagcagcg aaaaccccac ccatgcagcg gccctgggca
    39421 acggtgcaaa acatgcaatc agcagcatcg aaaccgtcca gggcaggagg tgcaggtggt
    39481 agcggcgcaa aagtcggcgg ccctccggcc cgtaccggac ctcccgtgcc agtgcaagcc
    39541 cgaagaggtg atcacggcgc ggcaacagcg ggagtgcgtg cagcaccgcg aggatgaaga
    39601 gttgcggcat caccgggagc aagcgaatcc agatcatcat gatgtcatcc tttctatggc
    39661 cgcgcgcagc tcggcggcga tctcaccagc ggacaatcct tcggcgcgca ttcgcgccac
    39721 catctcgcgg agacgctggc ggaaggcaac taccgcttcg cggctggccc tcgccggctc
    39781 ctggcgatcc actaccacga cgcggcggcc atgcctcaga tcaagccagc cttcccctgc
    39841 gagctgccga taggcttggg cgactgtatt gaacgtgatg ccaagctcca tggccagtcg
    39901 gcgaaccgaa ggcagagtgt cgccgggcgt cagattgccg tccacgaggt gggtgcgcag
    39961 ggcgtcggcg atctgccggt actgcgggac cggcgactga aggtcgattc ggattacggg
    40021 cgcccgtttc gtcatctgta tgctgtgcga ttacagagta cagttagaat acctgtttgt
    40081 caataccatc ccgaggcggg cctgacactt gccagtcaga atggccgcgc gagtgggagt
    40141 gcagacagga gccgccgcgc aggcctcggt gaaccgcacc ccggcggctg ccaggcggtc
    40201 gatattcggc gtttcgtgga ggtccgcgcc gtaaacgccc gtgtccgccc agccgaggtc
    40261 atccgccaga atgaaaaccc agaatgaaag caatgttgag cctctcgggc ggcttggacc
    40321 ccggcaaaga ccacaggcgc ggccagagcc gccggcccga gaaagtcgcg ccgtgtgtag
    40381 gctcgaagtt tcaaggcggc ggtattgtat cataagggtt tgcgtgacct ggaggtcgcg
    40441 atgaaaggtg tcgttctggc ggggggtacg gggagtcgcc tgtatcctct gacaaaaatt
    40501 acaaacaaac atctgctgcc gatctatgac aagccgatga tctactaccc catccagacg
    40561 ctggtggatg cgggtattca agagatactg atcgtcaccg gcggcaagaa cgccggcgac
    40621 tttctgcggc ttctggcaaa cggcaaggaa ttcggtctga gccacctgaa ctatacctac
    40681 caggagggcg aaggcgggat cgcagaggcg ctggggctcg cggagcactt cgccgatggc
    40741 gagaaacttt gcgtaattct tggggataac ctcatcgaag gcagcatccg ccacgcggtg
    40801 gacgacttcc ggaggcagcc gaaaggcgcc aaggtcctgt tgaaggaagt ccccgacgcc
    40861 cagcgctttg gcgttgccga actggtcggc gaccggatcg tcggggtcga ggagaaaccc
    40921 cgaaaaccga aatcgaacta cgccgttacc ggcttttata tgtacgatcc gacggtgttc
    40981 gacaagatcc ggacgctggt cccctccgac cgcggcgaac tcgagattac cgacgtcaac
    41041 aacgcctacg tcggggaagg cacgatgagc ttcgcattcc tggacggctg gtggaccgat
    41101 gccggcacgt tcgactccct gctgcgcgcg gccaatttcg tggcaaggtc gcgccatgcc
    41161 cagccggaac cggctgtggc cgaagccacg gtttaaggcg cgcgggttag acctgcggca
    41221 atcagcaaca atgaagcttc tggtgactgg gggcgccggt ttcatcggct ccgcttttgt
    41281 gcggctcatg atcgatgccg gtgcgtccgc cgacatcctg aatcttgata agctggctta
    41341 cgccggcaac ctggagaacc ttgtggcggt ggccggtcac ccacgccaca actttgtccg
    41401 tggtgacgtc tgcgacgcag cgtgcgtgga ggaacaagtc tccagtttcc ggccggacgc
    41461 catcgtccac ttcgcggccg aatcgcacgt agaccggagc attctgtcgc ccgcgccggt
    41521 attcgagacc aacctccgtg gaacgttcac gctcctggag gcggcgagaa agcatcatgt
    41581 cccccgcttc atccaggtct cgaccgacga ggtgtacgga agcatcgcgg agccgcttgt
    41641 ggcggacgag aagttccctc tgcggccgag cagcccgtat tccgcctcga aggccggcgc
    41701 ggacctcttg gcgctgtcgt acttcaccac ataccgcgtt ccagtaattg tgacgcgtgc
    41761 gtcaaacaac tacggtccct accagtttcc agagaagctg attccgctca tgatcgccaa
    41821 cgcgattgca gaccgcccgc tgccgattta cggcgacgga cttcaggtac gcgactggct
    41881 gtttgtggac gaccactgcc gcgctgtccg ggcggtcctc gaaaaaggac gcgacggcga
    41941 ggtctacaac atcggcggca actgttcctt gccgaaccgg gaagtggtac gccggattct
    42001 ggcggcggct ggtaagcccg aaacgctgat gactccggtg gaagaccggc cgggacatga
    42061 ccgccgctac gccctcacca gcgagaagct tattcgggaa acaggctggc agccagaggt
    42121 cgggttcgaa gagggattgg cccgaacgat tcattggtat ctcgcgaatc aggactgggt
    42181 gcgccacgtc cagtcgggcg agtaccttga gtattacacg cggaattacg gggaccgggc
    42241 gcaggagctt gcgcggctca ggaaacggta agttcgatga aagtggcgat cgatgccacg
    42301 ccccttacgc tctcgagcgg tggcatcgca cgatatacgg cggagttgag cgtcgcgctc
    42361 gcctcactgt tccccgaaga cgacttcgtc ctcctgtccg atcagccctt ctccatgccg
    42421 gcagtgggcg aaaaaaacgg ggacacttcg ggcttgcgct acagtgccgc acacctatcc
    42481 aggcgtgcag acgtgtcccc ggttttttcg cacccccc
//
LOCUS       DATIFQ010000476         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2459041, whole genome shotgun sequence.
ACCESSION   DATIFQ010000476 DATIFQ010000000
VERSION     DATIFQ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2459041"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /gene="hpnJ"
                     /locus_tag="VLE22_24225"
     CDS             complement(<1..1399)
                     /gene="hpnJ"
                     /locus_tag="VLE22_24225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HSB17579.1"
                     /translation="MRTLFLNPPSFEGFDGGASSRWPATREIESYWYPVWLCYPAGML
                     PDSKVVDAPPHKISIGQTVAMAGDFELLVLFTSTPGFHVDVKIAEMMRDANPKLKVAF
                     VGPPVTIEPEKVLRSSSAIDFVVRREFDRQIAGYANGEALDSMPGVSFRRNGDIVHNP
                     DGGMIEDLDSLPWVTKVYKRDLDITRYNVPFLLHPYLAFYTSRGCPAMCTFCLWPQTH
                     SGHRWRLRSADNVANEVRYAIENFPGLKEVFFDDDTFNYQKARTIELCRKLKPLNFTW
                     SCTSRVTTDYETLKAMKEAGCRLMIVGYESGDPQILKNIKKGATVDMARRFTENCKKL
                     GLLVHGDFIVGLPGETRQTLRNTIDFAKRLDVETIQVSIAHPYPGTEFYDYVKKNNLI
                     TIDSMTDEAGHQLPNIVYPGLDRPELVDWVERFYGEYYFRPRVAWRVVRRAIFDSGER
                     RRLYKEAREYLALRSK"
     gene            complement(1524..2342)
                     /locus_tag="VLE22_24230"
     CDS             complement(1524..2342)
                     /locus_tag="VLE22_24230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17580.1"
                     /translation="MFRLSGRILVVLAGLALPVLADDPAGLSAPSAPGGISSAEILNN
                     YETAVERQQQELGDLSMQVDISATLPRLKKEGSFHALRHVTRFGRITYDGVRFLGDKV
                     VKKDVIARYLTAEAEASNNRANGDGNGDVKVVSNGDGKATAKLASLAITQENYKFKYK
                     GMSEHEGRRVHVFQLSPKKKRVGLFKGELWIDPDSNLPVREAGRFVKNPSIFLKKVEF
                     VRDYELRDGLSLPSRILSRIDTRIVGRAELNILFSNVSVQSGAEQAAMVCPMGW"
     gene            2486..>2801
                     /locus_tag="VLE22_24235"
     CDS             2486..>2801
                     /locus_tag="VLE22_24235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HSB17581.1"
                     /translation="MAKVPVGTTREEKCLVTGEVAVDFLGMEEARVLGTPYLIAFMEI
                     TARNSVKSLLEPGQDTVGTLVNVKHLAATPIGMQVTFRAEVIEVDDRRILFKVEAFDE
                     REK"
BASE COUNT          573 a          790 c          887 g          551 t
ORIGIN      
        1 gcttggaacg gagggcgaga tactcgcgag cttccttgta cagacggcgc cgctcgccgg
       61 agtcgaagat cgcacggcgc accacacgcc aggcgacccg cgggcggaag tagtattcgc
      121 cgtagaaccg ttccacccaa tcgaccagct cggggcggtc gaggccggga tagacgatat
      181 tggggagctg gtgcccggct tcgtcagtca ttgagtcgat cgtaatcagg ttgttctttt
      241 tgacgtaatc gtagaattcg gtacccgggt acggatgagc aatggagacc tgaatggttt
      301 cgacatcgag gcgcttggca aagtcgatgg tgttgcggag agtctgccgg gtctcgccag
      361 ggagtccgac gatgaaatcg ccgtggacca gaagaccgag tttcttgcag ttttcggtga
      421 agcggcgagc catgtcgacc gtggcgccct tcttgatgtt cttgaggatt tgcgggtcgc
      481 cggactcgta gccgacgatc atcaggcggc agccggcctc cttcatggct ttcagggtct
      541 cgtagtcggt ggtgacgcgg gacgtgcagg accaggtgaa gttcagcggc ttgagcttcc
      601 ggcaaagctc gatggtccga gccttctggt agttgaaggt gtcgtcgtcg aagaagacct
      661 ctttcagtcc gggaaaattc tcgatggcat accggacctc attggcgacg ttgtcggcag
      721 accgcagccg ccagcggtgg ccggagtggg tttgcggcca gagacagaac gtacacatgg
      781 ccgggcaccc acgagaggtg tagaaggcga gatagggatg aagcaggaac gggacgttat
      841 agcgtgtaat gtccaggtcg cgcttgtaca ccttggtgac ccagggaaga gagtccaggt
      901 cttcgatcat cccgccatcc gggttgtgga cgatgtctcc gttccggcgg aagctcacgc
      961 cgggcatcga gtcaagcgcc tcgccgttcg cgtaaccggc gatctgacgg tcgaactcgc
     1021 ggcggacaac gaaatcaatg gcggaggagg accggaggac cttctccggc tcgatcgtga
     1081 caggcggacc gacaaacgca accttgagtt tcgggttggc gtcgcgcatc atttccgcaa
     1141 ttttgacgtc aacgtgaaac ccgggggtgg aagtgaaaag cacgaggagt tcgaagtccc
     1201 ccgccatggc aacggtctgg ccgatcgaga tcttgtgcgg aggggcgtcg acaaccttgc
     1261 tgtccggcag catccccgcc ggatagcaga gccagacggg ataccagtag gactctattt
     1321 cgcgggttgc cggccagcgg gaactggccc ctccgtcaaa tccttcaaaa gaggggggat
     1381 tcaggaaaag cgttctcatt caaacccctt gagtctacgg tactcggcgg caccggacaa
     1441 cccgacaccg gggaacaggc ccggcccatc attgagtggc gcttttccgc tatcgaagga
     1501 atcggccacc gacccggggc acactaccaa cccatcgggc aaaccatcgc ggcctgttcg
     1561 gcgccggact gaacactcac attcgaaaac aggatgttca actcggcgcg accgacgatt
     1621 cgcgtgtcga tacggctcag gatacggcta gggagcgaca acccgtcacg cagttcgtag
     1681 tcccgcacga actccacttt tttcaggaag atcgacgggt tcttcacgaa ccgacccgct
     1741 tcgcggactg gcaggtttga gtcgggatcg atccagagtt cccctttgaa caggcccacg
     1801 cgcttcttct tcggagacaa ctgaaagaca tggacgcggc gcccttcgtg ctcggacatg
     1861 cccttgtact tgaacttgta gttctcttgc gtgatcgcca gggacgccag cttggcggtt
     1921 gccttcccgt ccccattact cacaaccttg acatctccgt tcccgtcgcc gttagcccgg
     1981 ttgttgctgg cttcggcctc cgcggtcaga taccgggcga tgacgtcttt cttgaccacc
     2041 ttgtcaccga ggaaccggac gccgtcgtaa gtgatccgcc cgaatcgcgt gacgtggcgc
     2101 agggcgtgga agctgccctc ttttttgagc cgcggcagag tagcgctgat gtcgacttgc
     2161 atcgacaagt cgcctaactc ctgttgctgc cgctcgacgg ctgtctcgta gttgtttagg
     2221 atctcggcgg aagagatgcc gcctggcgcc gacggggcgg acaggccggc aggatcgtcg
     2281 gccaagacgg gcagggccaa gccggccaga acaacaagga ttctccctga caatcgaaac
     2341 atcaagcctc taccggttgg gtggcgaaag catcccttgc gcgggatccc gcttcctcaa
     2401 ctctaccaat agtaggatgc agacggcaac cgctccgttg cggggcttcc cgcacgccga
     2461 ccatattcga cggtacgata gcagcatggc taaggttccg gtcggcacaa cccgcgagga
     2521 gaagtgcttg gtgacggggg aggtggccgt tgactttctc gggatggagg aggcccgggt
     2581 gctaggcaca ccgtacttga tcgctttcat ggagatcaca gcgcggaact cggtgaaaag
     2641 cctgctagag ccggggcaag atacggttgg aacactcgtg aacgtgaagc atctggcggc
     2701 gacaccgatc gggatgcagg tgacgtttcg cgccgaagtt atcgaggtag acgatcgacg
     2761 tatcctgttc aaagtagagg cattcgacga aagggagaag g
//
LOCUS       DATIFQ010000477        34350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2652916, whole genome shotgun sequence.
ACCESSION   DATIFQ010000477 DATIFQ010000000
VERSION     DATIFQ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 34350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34350
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2652916"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VLE22_24240"
     CDS             complement(<1..1093)
                     /locus_tag="VLE22_24240"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HSB17582.1"
                     /translation="MRLSARYTGPIACPHCQGTRRRNKGRRIRYLRHESWGTRHCVVA
                     LETHKWLCRGCGRSFWQRFPGILPRKRATEPFRRSVFLKHWDGISRSRLGEREAIGSA
                     TVERWFEDFLRKLATERSAAACPQVLGVDEHFFSLRHGYATTFCDLRKHRVFDVVLGR
                     SEASLERYLERLEGKSAVRVVCMDLAAGYRALVRKHFPNARIVADRFHVIRLINHHFL
                     ACWRELDPRGSKHRGLRSLMRRHRHKLSPEQQARLAAYLAPHPVLELIYRFKQRLCYL
                     LLRKHRTRKQFQKLIPRFLRAVYQLRQAGLAQLVQLGETLSSWSEEIVAMWRFTRNNG
                     ITEGFHTKMEVLQRQAYGFRNFNNYRLRVK"
     gene            1315..2613
                     /locus_tag="VLE22_24245"
     CDS             1315..2613
                     /locus_tag="VLE22_24245"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSB17583.1"
                     /translation="MRRMLAALIFAGVLTCAEVQVGRIEVLVAPDHPDWKYVPGEKAV
                     FHIRAVRNGSDITGAEVTYSVGPEVMPVATTRTVALERGGLRIDGGTMQTPGFLRCIA
                     KVTDGGNTYQGIATAAFAPEEIQPVVRDPEDFDSFWASAKEELAKVPLDARRTLIPEL
                     STAVVDVYHVSLQNTGRSNVFGMLALPKAAGKYPAILGVPGAGVHKIGPMIELAEKGA
                     ITLAIGIHGIPLTLDPDVYAALANGALNGYWTYNLDSRERYYYRRVYLGCVRANDYLT
                     SLPEWNGRDLAVTGGSQGGALSIVTAGLDPRVKALAASYPALSDMAGYTEGRAGGWPH
                     VFRAAGEGSHRTPEKLATAAYYDVVNFARRLKAPGMYSWGYNDQTCPPTSMYAAYNVI
                     RAPKQLVLALETGHGRVPEQTDVINGWIQEFIKTGQPLVK"
     gene            2691..2996
                     /locus_tag="VLE22_24250"
     CDS             2691..2996
                     /locus_tag="VLE22_24250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17584.1"
                     /translation="MDNPQQRASEFSDRHDVVKTVCVWRNGTCYRIEIIRRAGKQEEG
                     AYAALLWLEEEQAGRRVLVRDVSFPWVHHESAESALDHALDSLETKLKYASTGETGV"
     gene            complement(3108..4016)
                     /locus_tag="VLE22_24255"
     CDS             complement(3108..4016)
                     /locus_tag="VLE22_24255"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HSB17585.1"
                     /translation="MASIGEILRQERRRRNLGVAQLADQTKIGAKILEAIEAEQWDRL
                     PGKFFAKSFIRQYAAALGVDADKVEAELERLFEVERPAAEAETEAPMERFPLPPVSPR
                     LEESRSGNRRWLLSLLVVTVVALCSGVYALWLSTRSVTAPSSPQPAVPPPATAKSRAP
                     VAETESNVPPPASESTTYATPAESATSAQQAPPVGSGNAEQSVSQESAPVTTPAGNLL
                     LNLAASEKTWLSLWVDGRQIFSGILVPGETKTIEGTSKARLYVGNAGGLEIHLNGKPV
                     GPIGKSGEVRVVDLTTEGIQIHREAI"
     gene            4191..5084
                     /locus_tag="VLE22_24260"
     CDS             4191..5084
                     /locus_tag="VLE22_24260"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSB17586.1"
                     /translation="MERKKATDFPQELLNLFDRYVHGDIGRRDFLEGAQKFTAGGLTA
                     TAIWESLRPNYAWAQQVPKDDSRIKTEYATVPSPQGNGSIRGYMVRPAGGGGKLPGVM
                     VVHENRGLNPYIEDVARRLGTENFLAFAPDGLTSVGGYPGDDEKGGQLFRQVDREKMT
                     EDFLAAIRWVKSRSDCTGKVGVVGFCFGGGIANTLAVRMGADLAAAVPFYGGQPRTED
                     AAKIKAPLLLHFASLDTRVNAGWPAFEAALKSNGVNYTAHMYEGANHGFHNDTTPRYD
                     EAAAKLAWQRTLDFLNKHLRG"
     gene            6091..6663
                     /locus_tag="VLE22_24265"
     CDS             6091..6663
                     /locus_tag="VLE22_24265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB17587.1"
                     /translation="MRIRRFALLSVLLWGDLGGIAAQKAPKRVPDVRYEPSSPEVVRQ
                     MLGLAGVRKADVLYDLGCGDGRIVIAAAKQFGTRGVGIDIDPERIKESTANARAAGVH
                     RLVRFRNEDLFEADIRKATVVTLYLWPWVNLKLRPKLLKELKPGTRVVSHSHDMGDWT
                     PERVIEVEGHKIYLWTIPGHGNIGDSDQGK"
     gene            6677..8002
                     /locus_tag="VLE22_24270"
     CDS             6677..8002
                     /locus_tag="VLE22_24270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HSB17588.1"
                     /translation="MADTVLRRTFVGVASALALRGAPGSRSTDRPAMLGGDPVRSKRF
                     PSWPQSFPADEKSWLDVVRACKWWRKEGHYVRDFEKAWAERMGARYCIATANGTGALL
                     ASLHALEVGPKDEVLVGPYTFIATVNAILGQFALPVFVDTDPDSMLIDANKIEAAITP
                     RTRCIMPIHLGGNVADMDRILEISKERNIPVIEDACQAHLSEWRNKKASTLGDLGCFS
                     FQKFKNLPGGEAGGVITNSERLYRHAYGYHSHYRTPDEGPMDAAASRNGINLRMAEFQ
                     AALLLAQLTRLEEQSRTREQNAAYLGELLSDVPGVKPAKMYSGCTRNSYHLYMMRYST
                     DAFASLPRARFVQAVQAEGIPVSAGYGPLNKDRFLENTLNSRTYRAVYTTQEIARWRE
                     RNHCPGNDRACQEGLWLGQNVLLGSRSDMEDIATAMRKVKRHAEALKKT"
     gene            complement(8199..8825)
                     /locus_tag="VLE22_24275"
     CDS             complement(8199..8825)
                     /locus_tag="VLE22_24275"
                     /inference="COORDINATES: protein motif:HMM:NF039204.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HSB17589.1"
                     /translation="MLGVPVTRFVAGLCGLFQVAVGCGEAPTALRATLTSQGAQNRRA
                     NADNLSRMRDVSMVKRFVRRGLLVRVPTSTRHYYLKSIPASYRYLRPWSKLFLDRLSR
                     QYHARFQRKLRVTSLVRTVPYQRALQSRNQNAAAARGSKRSSHLTGATLDISKAAMRP
                     AELAWMRRVLLALKKAGYLYAVEEFRQPTFHILVFRNYPSYVKAVSSR"
     gene            complement(8932..9423)
                     /locus_tag="VLE22_24280"
     CDS             complement(8932..9423)
                     /locus_tag="VLE22_24280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17590.1"
                     /translation="MSASERRSKESTGLIVAVVMLLLAPLVVYVAMTSGDQPEVRDES
                     PVRPTSLEQPAPRARVVTSTDLDREERLKNRKQVSKPAIPAPAPPVKRQRKAFPTPEA
                     IPVGTRKSQLIATFGTPSMITTAVDEGRATETFVYLQRDVEMGTVVHLRSGVVVGTGS
                     TPY"
     gene            9593..10582
                     /locus_tag="VLE22_24285"
     CDS             9593..10582
                     /locus_tag="VLE22_24285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSB17591.1"
                     /translation="MTQYTGRSISNVTTAGRSGVRIVRMPYTPNRRSFLRKSLAVAGG
                     VAAGSTFDHLPLVARLRGETTAAADLSSGKPPAGKIGKLEVTRLICGGNLFSGFAHSG
                     DLLYVSSLLKHYFTADKVLDTLQQCEEAGINTAILRTDDHIIGIIKRYRKERGGKIQW
                     IAQTYPKTDNVRQNIQMAIDNGAVGAFPMGGIADTWHKEGRTELIGEAIAFMKQNGLV
                     AGIGSHSLEVPKSAEAMKLNPDFYFKTMNKVGYESQEPAEVAALMKTIKKPWIAFKIL
                     GAGRMTPKDGFDMAYGLGADFVNVGMYDFQVKENVSLAREIISSHARRDRTWV"
     gene            complement(10601..11410)
                     /locus_tag="VLE22_24290"
     CDS             complement(10601..11410)
                     /locus_tag="VLE22_24290"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSB17592.1"
                     /translation="MKRSAEAAKWSWTTKRSWSRLGSSLVFAALGASLCFAQQRTILA
                     VGAHCGDAEITTGAALLKHKKLGDRIAILHLTLGEGGNPRMTPAAYGEQKRKEALSAA
                     KVLGADVIFGPYKDGELPVSDEAAQYVANVIRQVKPTHVLTHWRNSLHKDHTAAHRIV
                     NDAVLLASLEGFVTEHPRHRGVRGVYYAENWEDAEEFKPYLYVDVTGELGEWKRCVTQ
                     YEFIRGGISSFPYLDYYEALARIRGAESGKRHAVVFDVDPWSKRRVIDSLP"
     gene            11498..13573
                     /locus_tag="VLE22_24295"
     CDS             11498..13573
                     /locus_tag="VLE22_24295"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HSB17593.1"
                     /translation="MTRRSFFFSKIAGVTALHAATFARAAEPATPVSLPRAKWIEQGI
                     IDAGGSHEPYSFVVRRGGHRLDAYRRYQQTQSEEVIRRLKDNGVEVFHTHFYKGAGMV
                     HEKAEMEDAKRVAEIAHRYGLKVDSYLQWNTMLYETFFAEEPRAKDWIQRDADGRPIL
                     LRYGHQQSFRYRPCFSNPEYLEYLKKLVRYAVEEVKTDFIHFDNYDLSPEPDSCHCPN
                     CVRGFRDFLRRKYTPKRRKERFGFDNVDYVNPPRWNRPNPPEKMQIIYDPGIQEWIDY
                     RCHSMAEALRQMAEYAKSMNPEVAIEVNPHGITGGNRAWEAGLDHARFLNYTEVFWTE
                     EDNKPAYRPDGRLTSTIRSYKLARAFRNVLLTYISGDPASMAECLSFNQTIGFAGSDP
                     IAPEMLKYIAFYRKHRDLYVGAQDAGTVAVLRSFASITYNQPRVQLGTILMEQALIQA
                     RIPFDLVFDQHLKDLSKYRVLILPETECLSDEQLALVRRFVENGGGLIATGQAGMYDE
                     WRRVRVQPGLEGLINIKAEAVGYQERVEQTEISGAGVRKEVAKGRVVYFPAVQFDGPL
                     PEHEDYFEIGSRFWKLPKNWMEITESVRWAAREEIPATITGPLHLVANVVDQPEKRRR
                     MIHLVNYNSGKVPEVAGVRVTCRLPHGAAPKEVTLYSPDLNAPRTLPTANTIAVPPVK
                     VYSVVAVSW"
     gene            complement(13583..14758)
                     /locus_tag="VLE22_24300"
     CDS             complement(13583..14758)
                     /locus_tag="VLE22_24300"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSB17594.1"
                     /translation="MLILVLLLALTAAPLQAPDPTQRLNQHRVSQKRSPAPEPGAEKE
                     IIPSLVEDLHTPKDWYKKRRPEILKSWTTMLGKLEPKGPDKRWFGDIRKARIIDKQER
                     PAYTRIHLELPMEKDFWQPHLLLLPKNQGPGPFPAVICWTSSSPDWQKPEEWWGSWLA
                     GRGYVVLTGWSFIRNYRDGTSAGKGVPEKLYERFGHWLPLGKMVHDARREAEYLRSLK
                     QVDGRRLGFIGFSLGAKAAVYVAAFAPEFKATVALDPHIAVNGGTNWYAPWYLDWLRP
                     FPGIPTAERTPLSMLNPNPKRPGFEHDHHELMALVAPRAFLLIGGMNQREDNGGDSDD
                     LQSWGYFNRAREVYQLLGIPERIQFAHTGDGHNATGPGIDPAWQSFFERWLKQTPVQ"
     gene            complement(14758..16179)
                     /locus_tag="VLE22_24305"
     CDS             complement(14758..16179)
                     /locus_tag="VLE22_24305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB17595.1"
                     /translation="MILKQYYLGCLAHASYLVADEESHTAAVVDPRRDVDEYVEDARQ
                     LDLAIRYVFLTHFHADFVAGHLELRDLAGATICLGARAKAEYDFTALADGNAIEFGKT
                     RLTVLETPGHSPESICILVHDLAKDTERPHAVLTGDTLFIGDVGRPDLRASLGWTAEE
                     LGRMLYDSVRNKLLTLPDETLIYPGHGAGSMCGKNLGTETVSTIGTQRRYNYALQPMS
                     REQFVSIVAADQPEAPAYFTYDAVLNTKERPTLDKTLQQVLKPLSPEELLHLRSGGAQ
                     LLDVRNPADFAGAHLNGSINIGLGGQYATWAGTLLDREKPIVIIADPGSELEAATRLG
                     RIGFDNVTGCLHGGMQAFEALPGPLNRTARIATATLAEQLQSAKPPVVLDVRTPREWE
                     QKHINGSRNIPLNHLPERLAELPRDRLVVVHCQGGYRSSLAASILERHGFQQTADLTG
                     GIAAWEASGLPVSSSTFKKEAGA"
     gene            complement(16237..17049)
                     /locus_tag="VLE22_24310"
     CDS             complement(16237..17049)
                     /locus_tag="VLE22_24310"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HSB17596.1"
                     /translation="MGLHIKSPGDAPGVYPIVKRGEGLKHLSFTIIELGGALRTHEWE
                     TGEEEQSLDSYAGPVRVEVENSSGKWSAEIGARRTLKETGPMIYLPAGSKAKISRLDE
                     RARVTAAGAAGKTGASPRLIAGSEIMVTPTGKNNFARTVCTHIADNVDAVHLICGETV
                     SQPGGWTSSPPHKHDRFTAAGEVPMEEVYYFLFEPNQGFGFIRVYTGADDPEPFDHAF
                     VVEHGDTVLIPRGYHPVVACPGYTLNYTWVLAGEGRAYGTWSDDPRHTWIKD"
     gene            complement(17095..17490)
                     /locus_tag="VLE22_24315"
     CDS             complement(17095..17490)
                     /locus_tag="VLE22_24315"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB17597.1"
                     /translation="MRALDTNVLVRYLTADDSRQLVAASRFIDRCNQDGEFIFLPALV
                     LCKLVWVLFRCYDQSKAEIVAILERILQTEQFKIEHDALVRRSLEGYRNGKADFSDYL
                     IGEISRASGCPDTVTFDRALKGTPGFTML"
     gene            complement(17487..17729)
                     /locus_tag="VLE22_24320"
     CDS             complement(17487..17729)
                     /locus_tag="VLE22_24320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HSB17598.1"
                     /translation="MLTATLTSKGQITIPKEVRDRLRLRSGQRIEFQFDLAGQVTMKP
                     RTLDVRGLRGIVRPARKRAVTVREMNEAIAEGYSKA"
     gene            17804..20008
                     /locus_tag="VLE22_24325"
     CDS             17804..20008
                     /locus_tag="VLE22_24325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HSB17599.1"
                     /translation="MALALSFRFTSSAPARYHGAIVAMRFLVCFLLLALGAPAQQLTL
                     DRLFRRPFLWGTSPEQVTWSKEGHTLAFLWNTSGRRFLDLYAYHPGSRKLTRLTDLEP
                     VKDDLNLTEEEKDDRQKSYLEPLAGLGSFELSRDSSHAAFSYRGDLYVVETQGEKPPF
                     RLTRTKASESSPQLSPDNTRISFLRDRQLFVQDLRDGRLWQVTDIEEPDQAITSYRWS
                     PDGTRFVVAVRKGRGRQMVLPNYSGRFVKAGSFSRSVAGDEPVETALSIVPVEGGKPL
                     PVDLGPLGAKVYLGLVDWSPDSSQLLVRSVHPKVKQAHLLVIDAKTGKSVVLQEETDS
                     KWVHWTSAAWSPDSRNVVFTSDREGWAQLYRIPATGGKPEQLTRGAWEAQDPQWAGEN
                     IYYTSTEGGAAERHFYRIRPDGSGKQKLSRREGLNYGLVSEDGMHVAWLQANLSNPSD
                     LYVDEDRVTTSPLPEFANYRWPESRFFQYPSRRDGKSVAARLMLPPGYRQDDKSGKRW
                     PAVVYIHGSGYATSVLKEWGSYDPYRFAFNTYLAQRGFVVLEPDYRGSSGYGRDWRTD
                     VHLHMGGPDLDDVLGGLDYLRGLGDIDMKRIGIWGVSYGGFMTNMAMFLATDEFRAGV
                     SWAGVNDWENYNAWYTSERLTTPEENPEAFRRSSPIHFSRLLKNPLLIVHGMVDDNVL
                     FQDAVQLTEKLVQEGRPFWHIYYPQEDHAFVRDETLVDAYSRSAEWFERHLR"
     gene            20005..20640
                     /gene="thiE"
                     /locus_tag="VLE22_24330"
     CDS             20005..20640
                     /gene="thiE"
                     /locus_tag="VLE22_24330"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HSB17600.1"
                     /translation="MKLPRLYPILDTATLEKQACSIELAAEAMVAGGARILQFRHKGH
                     FSRALFEQAERVAALCRREGAIFVVDDRADVAMMLDAGLHVGQDDLPPSDARRLVGTE
                     KLLGFSTHNEVQLRAAAAEPIDYVALGPVFGTASKDRPDPVVGVEGLRRLRPLATRPL
                     VAIGGITRENALTVLDAGADSVAVIGDLLPEKCTFETLRTRMGLWQQLVTT"
     gene            20616..22013
                     /locus_tag="VLE22_24335"
     CDS             20616..22013
                     /locus_tag="VLE22_24335"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB17601.1"
                     /translation="MATTRDDVNTGLVKGLGLLDATTIVMGSMIGSGIFIVSADMARQ
                     TASPGLLILTWVISAVLTIIAALSYGELAAMMPQAGGQYVYLREAFGPLFGFLYGWTL
                     FLVIQTGTVAAVAVAFAKFTGVFFPWFSAGNALVRFGGFTINTQQALAIAVLVFLTWV
                     NTRGLRTGAAVQNVFTVAKTGAVLGLIGLGFLVGGAGGSGNFEGFWRNAGWNFDAVRI
                     VGVAMVGALFAADAWNNITFTAGEVRNPRRDVPLALGMGVGVVCLLYIACNFVYLRLL
                     PLEAIQTAAEDRVATAAASMIFGPVAVQVMAAAIMISTFGCINGMVLAGARVYYAMAL
                     DGLFFKAVANVDPVHHTPVVSLTVQCVWACLLTLSGTYSNLLDYVIFAVLLFYILTIA
                     GVFVLRRKRPDAPRPYKAFGYPVLPALYIVLAGVVEVLLLLYKPKYTWPGLAIVLLGL
                     PVYFVWRRRRTSALS"
     gene            complement(21946..22788)
                     /locus_tag="VLE22_24340"
     CDS             complement(21946..22788)
                     /locus_tag="VLE22_24340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17602.1"
                     /translation="MLRSSVLIAVLTALPAFCQPELRLQLRSRVEAFKGSAQWEEVRF
                     EKSLAVPQTAVLICDMWDNHWCRGAAQRVNEMVKRMAPVVEAARTGGIRIIHSPSDVM
                     DFYKDDPQRKRMLELPQVLPPEPMTFSDPPLPIDDKEGGCDTGDQDHKAWTRQHAGIR
                     IAPEDVISDKGTEVYSYLRANGIGNLLIMGVHTNMCVLNRTFAIKQMTKWGVRCVLVR
                     DLTDAMYDPKKSPFVSHARGTELVIEHIEKYWCPTVLSADLTKALRSASAAKRSTPAG
                     RATQ"
     gene            22894..24300
                     /locus_tag="VLE22_24345"
     CDS             22894..24300
                     /locus_tag="VLE22_24345"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSB17603.1"
                     /translation="MAESEGGEHTLKRTLGAMSLVALGIGAIIGAGLFSLTGIAAAEN
                     AGPAIALSFIVAAIGCAFAGLCYSEFSTMIPIAGSAYTYAYATMGEFLAWIIGWDLVL
                     EYAVGAATVSISWSAYIVSLLHDIGINCPPYLVASPWQPVQLPNGQLVHGLINLPAVF
                     IIALVSTLLVVGIKESANVNAVMVMIKVAVVIIFIGVGIWFIKGSNYVPFIPENTGTF
                     GEYGWSGILRAAGVIFFAYIGFDAVSTAAQEAKNPQKDMPIGIIGSLLICTVLYVAFA
                     FVMTGLAHYTEFKGAAAPVAVAIAKTPYMWLGSLVKFAIIAGLTSVILVMLLGQSRVF
                     FSMSRDGLLPGVFSEIHPKFRTPWKSNLLLMIFVSLFSAFAPISAVGHMTSIGTLFAF
                     VIVCVGIIIMRRTQPGLPRPFRTPWVPVVPVLGILVNAMMMYGLGWENWMRLFVWLAI
                     GLTIYFLYGRVHSHLARK"
     gene            24384..25328
                     /locus_tag="VLE22_24350"
     CDS             24384..25328
                     /locus_tag="VLE22_24350"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HSB17604.1"
                     /translation="MPLASALGLGMLAGARLYATVFVVGLLLRLDWIAVPTAWQHASV
                     LADTRVLVASGIACALEFIADKIPWVDSAWDSIHTFLRPIGAALLTSSLFSNLEPAYQ
                     VLLFLLAGGVAFSGHSAKAATRFVVNHSPEPFSNVAVSLAEDAFVAGGLYMLVKHPWI
                     MAGIVLLFLALFAWLAPRVFRALRAEWAALTAVIRNWTGDSVQPSLAPAEREWLAANH
                     PGRQPGEVFCVIATSDLKGLRNAIGTLCLIGGDAAFFARRWMRPVRKTIGTVTGTETR
                     QGILVDRLILALADGSRIRFDLLAGQMPKAAQFVQPAA"
     gene            complement(25341..26054)
                     /locus_tag="VLE22_24355"
     CDS             complement(25341..26054)
                     /locus_tag="VLE22_24355"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSB17605.1"
                     /translation="MTQTGTAPLRILSIDIGGSGVKAIVIDQKGRPLTERTRLETPRP
                     ATPAAILRTIDRLAAAQRPFDRISLGFPGVIRKGVAYTAYNLHDRWVGFDIAAALRKR
                     LHKPARVANDADIQGMGAVRGRGVELVLTLGTGLGSALFLEGRLVPNLEVAHHPFHKG
                     LTYEEWLGKAGYDELGVKHWNRMLARAVAELEHLFNYDWLYLGGGNAKKVSLQGLPPN
                     VKVVPNVLGLLGGAALWRK"
     gene            complement(26137..27072)
                     /locus_tag="VLE22_24360"
     CDS             complement(26137..27072)
                     /locus_tag="VLE22_24360"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB17606.1"
                     /translation="MLKLGFVTAILPDLPLREVASFASASGYSCVEVMCWPKGKAERR
                     YAGVTHIDVVGLTAADAARINDAVKTAGVAISGLGYYPNPLVPDSAEAQVYIDHLKQV
                     ILGARLLGVGVVNTFIGRDWTKSVDANWPRFLEVWKPLIRLAADNGIRIGIENCPMSF
                     SADEWPGGKNLAVSPAIWRRMFNDIPDNNFGLNFDPSHMIWQRMDYLKPLREFASRIF
                     HVHAKDARIDQERLDEVGILAYPLQYHTPKLPGLGDVNWGRFFSVLTETGYDGPVCVE
                     VEDRAFEGSLENRQAALRQSANYLKQFISTAALSA"
     gene            27192..27860
                     /locus_tag="VLE22_24365"
     CDS             27192..27860
                     /locus_tag="VLE22_24365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17607.1"
                     /translation="MNKTRFFVTVPVASVLLALTACSSPPPQEKAAEVKKSPGPAANA
                     GASQLTRDPLPGCEWRGSVDPSIRLYILVQQCTDAAHKNSFLAYGNAIYQIPAAAGGE
                     IAKGKRIVEVFEKPAGQPIEQALQEQFIAKLPPPGKEGCLVRSPAEGVVVSDPTKVTY
                     EIVPGPEYKGGSEAGSRPCGEYGKADSPMYFEYHPRESRVRFLFVNPSGDPPMFDAQA
                     IRLR"
     gene            28233..29033
                     /locus_tag="VLE22_24370"
     CDS             28233..29033
                     /locus_tag="VLE22_24370"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HSB17608.1"
                     /translation="MINILLALAVLVAVSVAWSRLRFKPITVFEYERGLKYQKGRFET
                     VLQPGRYWVYLPHTSLVKLDIRPRFVTVPGQELVSSDGVSVKVSLAARYEVADAYVAV
                     LKNENYETALYLLLQVKLREIVGASKIDELLEKRNLLGQMLMESSEKPAEELGLKLHS
                     VNLKDIMFPGELKKVFAQAVKAQKEGLAALEKARGETAALRNLANAARMIQENPALLQ
                     IRLLQQLGETSGNTVVLGVPATTPLPIRSPQPSRPDTAQLGASEETNE"
     gene            29143..29469
                     /locus_tag="VLE22_24375"
     CDS             29143..29469
                     /locus_tag="VLE22_24375"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSB17609.1"
                     /translation="MNNSTYTHIADLIKEVEIPVNGILSRTLYNDDRLKVVIFGFAAG
                     QELTAHTAPMPAVIQILSGEARLKLGDDTIEAGPGTFVRMPPQLEHGVYATSPVVMLL
                     LMMKNA"
     gene            29512..30801
                     /locus_tag="VLE22_24380"
     CDS             29512..30801
                     /locus_tag="VLE22_24380"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB17610.1"
                     /translation="MKIPKLRWIIAAMLLLATMINYADRLALSVVSVDLRSEFGLDEQ
                     DYAQIVALFFFAYAIMYAGSGYIVDRLGTRRGFSVFIFGWSLAQMMHGFARGKWSLAG
                     CRFMLGLAEPGNWPAAAKAVAEWFPASQRALGVGIFNAGSSLGSAIAPPMVAWLTLMY
                     GWRSAFIFTGTLGLVWLTAWLLLYQPPHRNRWLREKEYLELRDQVRPPEETLPASRSA
                     LDWRKVITMRGCWTLIVARFFTDPVIYFVIFWLPEYLRRERGFDLAMVGRYAWVPFIF
                     GDIGYVLGGWLSGRLIKAGWKLPTARKFVMLLGAAVMPAAMFAPLVPAAWMAIAATCF
                     VTFGHAFWVANLQTLPTDLFPGHEVGTATGFSGMGGAIGGVLANLGTGWVVQHFSYTP
                     IFLLAGLMHPLSIVLVYWLLPSRFFRKAEIDPTPISG"
     gene            30956..31351
                     /locus_tag="VLE22_24385"
     CDS             30956..31351
                     /locus_tag="VLE22_24385"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB17611.1"
                     /translation="MRGADRRNGIRFPLKLNCRVVFPFKARCNFEGITENLSRDNVLI
                     RVPRERIRGTPLRVGDWVRIMLDLPGTESLPSRCLGCEATLARVEDDGPEAVHLAFQI
                     DQMEFCESENGEAAGRARVSQIALSGLAT"
     gene            complement(31365..32018)
                     /locus_tag="VLE22_24390"
     CDS             complement(31365..32018)
                     /locus_tag="VLE22_24390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB17612.1"
                     /translation="MNRTLPKPLRVLIVDDHALFREGVARVLTDQPNFAVESCESVDE
                     ALRLVEKQAPDVVLLDYDLGSERGSRFLPAARDLGFEGRVLVVTAWISDQEAVRLMRS
                     GVAGIFPKENPTSALVEAVVDVARGGAWLDQRFLKLVTGVEAGSDSAELPKHLSERER
                     KVVRALFEGLSNKEIAGRLRVSETIIKTVLQRLFQRTGVHTRGQLVRVMLEDYQDQI"
     gene            complement(32015..33448)
                     /locus_tag="VLE22_24395"
     CDS             complement(32015..33448)
                     /locus_tag="VLE22_24395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HSB17613.1"
                     /translation="MSSNAATPQGERRTSPFEGFLAYSEAHRTGVLALAAGLTLCIAL
                     ADWKIQPNVSLGVLYMVPILLASVTLRRWQIVTLSAACAVLREALGPFIGEPGMAARL
                     LIVAGGFSGAGLLVFELSHSRRLAVRNLKLREEAEQQLRVLIETSPLAIVTLDSSGTV
                     ILANHSANRLLGFLDGDALQGRDIGQYLPVLGRPLRNGDAGRSLRTTIECRGQRRDGE
                     VFLAQVWFSTYRIGGGARLAAVIWDASENLRDREDAGLKSLMSTSRVLIGAVSHEVRN
                     LASAAASAYGALEGRPALVGNKDFQALGALVKAMEQVATSGLRLAANRQPEWVDLETV
                     LDEARIIIESSFREFSVLVQWEAEPGLPLVRANHHSLLQVFLNLARNSERAMRESVSK
                     EFTVQAVRDRDTVLVRFRDTGCGVTDTANLFRPFQAGAEDSGVGLYVSRAIARSFDGD
                     LRYEPQATGSCFVVELRPASEERKGAP"
     gene            33687..>34350
                     /locus_tag="VLE22_24400"
     CDS             33687..>34350
                     /locus_tag="VLE22_24400"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HSB17614.1"
                     /translation="MRRVLRYGPAAVLILLVFVGVVLQGSLPVASINAWVAGGSPLAQ
                     RTGACAVTLADGNVLIAGGVDAGGNPLASAEIVSKFSGSFSAAASMLAARSRHACAAL
                     PDGRVLVMGGVVSGGVTNDTEIYDPSSGTWAAGPPMSGTGRAGHTASVLSDGKVVIAG
                     GENGSAVLASVEIYDPEANSFAPAGALNEPRKDHGAAATLDGWVWIGGGHNGTEELAS
                     AEV"
BASE COUNT         6503 a        10629 c        10533 g         6685 t
ORIGIN      
        1 ccttaactct caaccggtag ttgttgaagt ttcgaaaccc gtacgcctgc cgttggagga
       61 cctccatttt cgtatggaag ccttcggtga tgccgttgtt gcgggtgaag cgccacatgg
      121 caacgatctc ctctgaccag gaggacaagg tttcgccaag ttgtaccagt tgcgccaggc
      181 cggcctggcg cagttggtag acggcgcgca ggaaccgggg tatgagcttc tggaactgct
      241 tgcgggtgcg gtgcttcctc agcagtaaat agcacaggcg ctgcttgaag cggtagatca
      301 gctcgagcac cggatgcggg gcgagatacg ccgcgagcct ggcctgctgt tcggggctga
      361 gcttgtggcg atggcggcgc attaacgacc gcaggccgcg atgcttggaa ccccgggggt
      421 cgagctcgcg ccagcaggcc aggaagtggt ggttgatgag ccggatgacg tggaagcggt
      481 cggccacgat gcgggcgttg gggaaatgct tgcgcaccag cgcgcgataa cctgcggcca
      541 gatccatgca gaccacccgg accgcgcttt tgccctctaa gcgttccagg tagcgctcca
      601 gcgaggcctc gctgcggccc agcaccacat cgaagacgcg gtgcttacgc aggtcacaga
      661 aggtggtggc atagccgtgg cggagactga agaagtgctc gtccacgccc aggacctggg
      721 ggcacgccgc agcgctgcgc tcggtcgcca actttctcag aaagtcttca aaccagcgct
      781 ccaccgtggc actgccgatc gcctcgcgct ctcccaggcg gctgcggctg atgccgtccc
      841 agtgcttcag gaaaacgctg cgccggaacg gctcggtggc ccgcttccgc ggcaggatgc
      901 cagggaaccg ctgccagaag ctgcgcccgc atccccggca gagccacttg tgcgtttcca
      961 gggccaccac acaatgccgt gtaccccagc tctcgtgccg cagataccgg atccgccggc
     1021 ccttgttgcg ccgccgggtg ccctggcaat gtgggcacgc tatcggcccg gtataccggg
     1081 ccgatagcct cacctcgcct gcttccgagt cgattccggt aatctggtat ccaggcaggc
     1141 ctaagattgc ctctgtgctg atcaaaacgt ttcctcccaa ggatgttttg tcagtcagag
     1201 actacagcct gcccccatta atgatcaaga gccagcaggg ccgaccacac aggtacccac
     1261 catacgcctt gctcatggct tgaccgtgcg cgcgtcgtga gaaactggag cgttatgaga
     1321 cgaatgttgg cggcattgat ttttgcgggc gtgctgacgt gtgctgaggt gcaggtgggg
     1381 cgtatcgagg tgctggttgc gccggaccat cctgattgga agtatgtgcc gggcgagaag
     1441 gcggtgtttc acatccgggc tgtccgtaac ggcagcgaca ttacgggagc cgaggtcaca
     1501 tactcagtgg gtcccgaggt gatgccggtg gcaaccacga ggacagtagc cctggagcga
     1561 ggcggcctgc ggattgacgg cggtaccatg cagacaccag ggttcctgcg ctgcatcgcc
     1621 aaggtgacgg acggcggaaa cacgtaccag ggaatcgcga cggctgcgtt cgcaccggag
     1681 gaaatccagc cggtggtgcg cgaccccgag gacttcgact cgttctgggc atcggcgaag
     1741 gaagagctcg ccaaagtacc cctggatgcg cgccgaaccc tgattcccga actgtcgacg
     1801 gcagtggtag acgtgtacca cgtcagcttg cagaataccg ggcggtcgaa cgttttcggc
     1861 atgctggcgc tgcccaaggc tgccgggaaa tacccggcga tcctgggcgt gccgggcgcc
     1921 ggcgtccaca agatcggacc gatgattgaa ttagcggaaa aaggtgcgat caccctggcc
     1981 attggtattc acggcattcc gctgacgctc gaccccgacg tttacgcggc actggcaaac
     2041 ggtgcgctga acggatactg gacctacaac ctcgattctc gcgagcgcta ctattatcgt
     2101 cgggtctatc tcggctgtgt gcgggcgaac gactacctga cctcgctgcc ggagtggaat
     2161 ggccgcgatc tggccgtgac gggcggaagc cagggcggcg cgctgtctat cgttaccgcc
     2221 ggcctcgatc cgcgggtaaa ggctttggcg gcatcgtacc cggcgctctc cgatatggcc
     2281 ggatacaccg aaggccgtgc cggcgggtgg ccgcacgtgt ttcgcgctgc gggtgaggga
     2341 tcccaccgca cacccgagaa actggcgacg gccgcctact acgacgtggt caacttcgcg
     2401 cgccgattga aggcgccggg gatgtactcc tgggggtaca acgaccagac ctgcccgccg
     2461 acgtcgatgt atgcggcgta taacgtgatc cgtgcgccga aacagctcgt cctggcactc
     2521 gaaacgggcc atggcagagt gcccgagcag acggacgtaa tcaacggctg gatccaggag
     2581 tttatcaaga ccgggcagcc gctcgtgaag tagcgagcat cgcaagccgg ctgcccgatc
     2641 gcctgcgcgc gcgggaggcg gtcctcgcgc ccggtgctag actggtgcgg atggacaacc
     2701 cgcagcagcg cgcttcggaa ttctctgatc gccatgacgt ggtaaagacg gtctgtgtct
     2761 ggcggaatgg cacctgctac cgcatcgaga ttatccggcg ggcgggcaag caggaagaag
     2821 gcgcttatgc cgccttgctc tggcttgagg aagagcaggc gggccggagg gtccttgtga
     2881 gggatgtcag cttcccttgg gtgcatcacg agagcgccga atctgccttg gaccacgcgc
     2941 tggactctct ggagacgaag ctaaagtatg cctctaccgg cgagacggga gtctgaatcg
     3001 ccgccaacca ggaatcccgg gcgtgaaggt ctcgcgcatc aaccccatcg aggcgctacg
     3061 ctacgagtga cgccaaagtc cccgggagcg gagccggcga acgaaactta gatagcctcg
     3121 cggtggatct gaattccttc cgttgtgagg tcgacgacgc gcacctcgcc gctcttgccg
     3181 atggggccga ccggcttgcc gttgagatga atttccaaac cacccgcatt tccaacgtac
     3241 aacctcgcct tgctcgtccc ctcaatggtc ttggtctcgc cggggacgag gatgccgcta
     3301 aaaatctgtc gcccatccac ccaaagggaa agccaggtct tttcgctggc agccaggttc
     3361 aggagcaaat tgccggccgg tgtggtaacc ggggcgcttt cctgtgagac ggactgttcg
     3421 gcgttaccgc tccccacggg cggcgcctgc tgcgcggaag ttgcggattc ggccggcgta
     3481 gcgtaagttg tgctttcgct tgccggaggc gggacgttgg actccgtttc cgcaacggga
     3541 gctcgggatt tggccgtcgc tggcggcgga acggccggtt gcggggaaga aggcgctgtg
     3601 acgctccgcg tgctcagcca cagcgcgtag actccggagc aaagcgcgac gaccgtgacg
     3661 acaaggagcg aaagcagcca tcggcgattg ccggaccgag actcctccaa ccgaggggaa
     3721 accggaggca gggggaaccg ctccatcggc gcctctgttt cggcctccgc agccggtctc
     3781 tcgacttcga acagtctctc cagttcggct tccactttat cggcgtccac gcccagcgct
     3841 gcggcgtatt gccgaatgaa gctcttggca aagaacttac ccggcagccg gtcccattgc
     3901 tcggcttcga tcgcctccag gatcttggcg ccgatcttcg tctgatcggc aagctgcgcc
     3961 accccgagat ttcggcggcg gcgttcctgt cgcagtatct cacctatcga tgccatacca
     4021 gctttcgagg attgcccgtg tcttcaattc tactgcggtt cgtcgcgcct tcccaagtct
     4081 actcgcgttc ggaggagttc cccgtcctgg aaacaagacg gcgtcggaaa cggaggatgc
     4141 gtggacttga tggatggtat gttatcctcg gcactagaca gggggcttcg atggaaagaa
     4201 agaaggcgac cgattttccg caagagctgt tgaacctttt cgaccgctac gttcacggcg
     4261 atatcggccg acgcgacttt ctggaaggag cacagaagtt caccgccggc ggattgacgg
     4321 ccacggcgat ctgggagagc ctgcgtccga attacgcctg ggctcagcaa gtccccaagg
     4381 acgacagccg gatcaagacc gaatacgcga ccgtcccgtc gcctcagggc aatggaagca
     4441 tacgcgggta catggtccgt cccgcgggcg gcggcgggaa gctgcccggt gtgatggtcg
     4501 tacacgaaaa caggggactg aatccctata tcgaggacgt ggcgaggcgc ttgggcactg
     4561 agaatttctt agcgttcgcg ccggacggac tgacgagcgt cggcggctac ccgggagatg
     4621 acgagaaagg cgggcagttg ttccggcagg ttgaccggga gaaaatgacc gaggattttc
     4681 tggcggcgat acgctgggtg aaatcgcgca gcgactgcac cggcaaagtg ggcgttgtcg
     4741 ggttctgctt tggcggcggc atcgccaaca cactcgcagt gcggatgggg gcggacctcg
     4801 ccgcggcggt tccattctac ggcggccagc cccggaccga ggacgcggcg aagatcaagg
     4861 cgcctctcct acttcacttt gcctcgctgg acacccgcgt caacgcggga tggcccgcat
     4921 tcgaggcagc gcttaagtca aacggcgtga actacacggc gcacatgtat gagggcgcga
     4981 accacggttt ccacaacgac acgacgcccc gctatgacga ggccgccgcg aagctcgcct
     5041 ggcagcgcac tctggatttc ctgaacaagc atctaagggg ttgactaatc ccaacagcca
     5101 cacacaagat cagctcggcc gctgtcattc atttccgctg ttcttttgat ccagctattg
     5161 gatataactg gaaccgagaa accccaggaa agacgcgtcc ccgtccgaag cccgctgatc
     5221 ctgatcctcc acccggaatc gggcgatctg gcaagccacg gaaggctctg acgactcctc
     5281 gtcaaccacc ctgacgaccc tggtactgca gacgagacaa cgagccgaga agttgggact
     5341 gtgcggaagg tcaatgctga cttccgcgaa ctctccgatt gtgacgcaca gctcggcggc
     5401 cgcgactttc tccaagacga cgagcacacc cgaccggctt atgttccggg tgaaacccca
     5461 aaatacttgt gagttcatcg aggggaacaa aacgcggcag cgtagccgca gcgcgaaacg
     5521 tctcccctgc cgcttctcag agctggggat ttccagacca ctgcgcagcc cagcgccctg
     5581 catatgattg aggataacat agttccgcga ggaagggact agtattctag gaaagttctt
     5641 ccaacaaact ttgaattgta gaagttttcg acagcactaa cagtactgac gtatggcgag
     5701 ttatttcgaa aggaattcgc ctttgtcgtg aatctctgca cctcgcgacc tcaactatcg
     5761 cgatctcaac cttgctgatg ccgagggagg cggtcagttt cagacaactc aaatctgtaa
     5821 gagccatcgg tctctgaagc acatgtcccc ggccactaaa taacaggaac gcaaggccga
     5881 aaatcaggca ggcaccacct ataactcctg aactcggccg gatctgccgg ctcttcatgg
     5941 cggccaccgc agccagcggt gactgctctt catcccgggt ggcgaatcgt ttccctcgct
     6001 cgcctcgtcc atcggccgcc acggctcgat actcagccca ccgcaggtgt agactcagtg
     6061 agtcagttcc ttacggattg agaacgaaac atgcgaatcc ggcgttttgc tttgctgtca
     6121 gtgctgcttt ggggtgatct cggtggaatc gcggcgcaga aggccccgaa gcgcgtaccg
     6181 gacgtgcgct atgagccatc gtcgccggaa gtcgttcgcc agatgctggg gctggcaggc
     6241 gtacggaagg ccgacgttct atacgatctc ggctgcggcg acgggcgcat cgtcatcgcc
     6301 gcggcaaagc agttcggcac gcggggcgtc ggcatcgata tcgaccccga gcgcatcaag
     6361 gaatcaaccg ccaacgcccg tgcggccggc gtccaccggc tggtcaggtt ccggaacgaa
     6421 gacctgttcg aagccgacat ccgcaaagcc accgtggtga ccctctacct ctggccctgg
     6481 gtcaacctga agctccggcc gaagctgctg aaagagctga aacccggcac gcgcgtcgtc
     6541 tcgcacagcc atgacatggg cgactggacg ccggagcgcg tgattgaggt tgagggccac
     6601 aagatctacc tatggacgat tcccggccac ggtaacattg gagattcgga tcaagggaaa
     6661 taggaggttt ggtgcaatgg cagacacagt gttgcgacga acgtttgtag gcgtggcctc
     6721 ggccctcgcc ctgcggggcg cgccgggaag caggtccacc gacaggcccg ccatgctcgg
     6781 gggcgatccc gtccgttcga agagattccc ctcatggcct cagtcgtttc ccgctgacga
     6841 gaaaagctgg ctggatgtgg tgcgcgcgtg caaatggtgg cgcaaagagg gccactatgt
     6901 tcgcgatttt gagaaggcgt gggcggagcg gatgggcgcg cgctactgca tcgcgaccgc
     6961 caacggcacg ggcgcgctgc tggcgtcgct gcacgcgcta gaggtcgggc cgaaggatga
     7021 agtcctagtc ggaccgtaca cgttcatcgc taccgtcaac gcgatcctag gacagttcgc
     7081 gctgccggtg tttgtggata cggatccgga ttcaatgctg atcgacgcga acaagatcga
     7141 ggcggcgatt acgccccgga cgcgatgcat tatgccgatc cacctcggcg gaaatgttgc
     7201 ggacatggat cggattctgg aaatctcgaa ggaacgcaac atccccgtca tcgaggacgc
     7261 ctgccaggcg catctctccg aatggcggaa caagaaagcg tcgacgctgg gcgacctcgg
     7321 gtgtttcagc tttcagaagt tcaagaacct tcccggaggc gaagccggcg gagtgatcac
     7381 gaacagcgag cggctgtaca ggcacgctta cggataccac tcgcactacc gcacgccgga
     7441 cgagggaccg atggacgcgg ccgccagccg gaacggcatc aacctgagaa tggcggaatt
     7501 ccaggccgca ctgctactcg cgcaactgac acggctggag gagcagtcca gaacgcggga
     7561 gcagaatgcc gcctatctag gcgaactgct tagtgatgtg cccggcgtca agccggccaa
     7621 gatgtacagc ggctgcaccc gcaatagcta ccacctgtac atgatgcggt acagcacgga
     7681 cgctttcgcc agccttccgc gggcacgttt cgttcaggcc gtacaggccg aaggaattcc
     7741 cgtctcagcc ggttacgggc ccctgaacaa ggaccggttc ctggagaata cgctgaattc
     7801 acgcacgtac cgcgccgttt acacgactca ggaaatcgcc cgctggcggg agcggaacca
     7861 ctgtccggga aacgatcggg cctgccagga agggctctgg ctcgggcaga acgttctcct
     7921 gggctcaagg agcgacatgg aggacatcgc gaccgcgatg cgcaaggtaa agcggcacgc
     7981 cgaggcgttg aagaaaacct gagcaacgca ggcgccggtt taccgcttct gtcccggcag
     8041 ttccgaccgt tcaatcagac ccactcctta attgcgtggt actgaaaatc acgtagcggc
     8101 ccggcgatcg aacgaaggat gcagatgaac agtctccggc ttgggaccca gcagtaaacc
     8161 aacaatagca ggagcgctgc gttcgcggaa tcgaacggtt aacgcgagga aacggctttc
     8221 acgtaactgg ggtagttgcg gaaaaccaaa atgtggaacg tgggctgccg gaattcctcc
     8281 accgcgtaga ggtaaccggc tttcttcagt gccagaagta cgcgtctcat ccaagccaac
     8341 tccgccggcc tcattgcagc cttcgagatg tcaagtgtgg ccccggtcag atgggaagat
     8401 cgtttgctcc cccgtgcggc ggcagcattt tggttcctgc tctggagtgc ccgctggtag
     8461 gggaccgtcc ggaccaagct cgtgacgcga agctttctct ggaaccgggc gtgatattgc
     8521 cggctaagtc gatcgagaaa cagcttactc catggacgga gatagcggta cgaggccggg
     8581 atcgacttca ggtagtagtg gcgggtcgaa gtgggtacgc ggaccagaag gccacggcga
     8641 acgaaccgtt tcaccatgga aacgtcccgc atgcgggaca gattatccgc atttgcccgg
     8701 cggttctgtg cgccttgcga agtgagagtc gcccggagcg cggttggcgc ttctccacac
     8761 ccaaccgcga cttgaaacag cccacaaagt ccggccacaa accgggttac aggaacacca
     8821 agcactactc ccagattaac cggtgttcat ttcctgtcaa gacagaatcg cgaaatactc
     8881 gctgaggact tgcacgaccc tcagcgccgc ccgactcgac ggagtgcctc cctagtacgg
     8941 ggtggagcca gttcccacaa cgacaccgct tcggaggtgg acaactgtcc ccatttcgac
     9001 gtcccgctga aggtaaacga acgtttcggt cgcccgccct tcgtccactg ccgtggtgat
     9061 catgctcggc gtgccaaagg tcgcgatcag ttgcgacttc ctcgtaccaa ccgggatcgc
     9121 ctccggcgtc ggaaaagcct tgcgctgccg cttcacgggc ggtgcgggag cgggtatcgc
     9181 cggtttcgag acctgctttc ggttcttgag ccgctcttcc ctgtccaggt ccgtgctcgt
     9241 caccacgcgg gcgcgcgggg ccggttgctc caatgacgtt ggacgaaccg gcgattcgtc
     9301 ccggacctca ggctggtcac cagaggtcat ggccacgtac actacaagcg gcgcaagcag
     9361 cagcataacc accgcaacta tcagtccggt ggattccttc gatcggcgtt ctgaggcgct
     9421 catgttcgtc ttggggcgga tggccgcgtg ataacgcaac cctcccaaaa cattgtgcgg
     9481 tcggtgggac gttttgtaaa tgctgcaaaa ggttaacgga gtttatatca ccagggatag
     9541 tgttaacggg gcagtgtccc cccggcgagg gtggcagacg accaatccaa tcatgacaca
     9601 gtacaccggg agatccatct cgaacgtcac aacggccggc cgctccggtg ttagaatcgt
     9661 gcgcatgcct tacacaccga accgtcgttc gttccttagg aagtcgctgg ccgtagccgg
     9721 aggagtcgca gcaggctcga ccttcgatca cctcccgctc gttgcgaggc tccgtgggga
     9781 gacgactgcc gccgctgacc tttcctcagg caagccgccg gccgggaaga tcggcaagct
     9841 cgaagtcact cggctcatct gtggaggcaa cctcttcagc ggtttcgccc acagcggcga
     9901 tcttctctac gtatcgagtc tcctgaaaca ctacttcacc gccgacaagg tgctcgacac
     9961 attgcagcaa tgcgaagaag ccgggatcaa cacggccatc ctgcggacag acgatcacat
    10021 catcggtatc atcaagcgct accgaaagga gagaggcgga aagatccagt ggatcgcaca
    10081 gacctacccg aaaactgaca acgtgcggca aaacatccag atggcgatcg acaacggtgc
    10141 cgtgggagcg tttccgatgg gcgggatcgc cgacacgtgg cacaaggaag gccgcacgga
    10201 actgatcggc gaggcaattg ccttcatgaa gcagaacggg ctggtcgctg gaattggatc
    10261 gcattcgctc gaggtcccga aatcggcgga ggcgatgaag ctcaatccgg acttttactt
    10321 caagacgatg aacaaggtcg gctacgagtc ccaggaaccg gccgaggttg ccgccttgat
    10381 gaaaacaatc aagaagccat ggatcgcctt caagatcctc ggcgccggcc gaatgacgcc
    10441 gaaggacggc tttgacatgg cctacggcct cggcgcggac ttcgtaaacg ttgggatgta
    10501 cgacttccag gtgaaagaaa acgtatcgct ggcgcgggag atcatctcca gccacgctcg
    10561 gcgcgatcgc acctgggtgt agagccgagg ccgccgccag tcacggcagc gaatcgatca
    10621 cccgcctctt cgaccaggga tcgacgtcga acacgactgc gtgacgtttg ccggattcgg
    10681 cgccccggat tctcgccagc gcctcgtagt agtcgagata agggaacgag gaaataccgc
    10741 cgcggatgaa ttcatattga gttacacatc tcttccactc ccccagttcc cccgtgacat
    10801 cgacgtaaag gtacggcttg aactcctcgg catcctccca gttctcggcg taataaacgc
    10861 cgcgcacgcc gcggtgccgc gggtgttccg tcacgaagcc ctccagcgaa gcaagcagaa
    10921 cagcatcgtt cacgatccgg tgcgccgcgg tgtggtcctt atgcagactg ttgcgccagt
    10981 gagtcagaac gtgggtcggc ttcacctgcc ggatgacgtt cgcgacgtac tgcgccgcct
    11041 cgtccgatac gggcagttcg ccgtctttat acgggccgaa gatcacgtcg gcgccgagta
    11101 ccttcgcggc agacagcgcc tccttccgtt tctgctcgcc ataggcggca ggcgtcatgc
    11161 gtggattgcc gccctcgccg agcgtcaggt gcaggatagc gatccgatcg ccaagcttct
    11221 tgtgtttgag cagtgccgcg cccgtggtga tctcggcgtc gccgcagtgg gcgcccacgg
    11281 ccaggatcgt cctttgctgc gcgaaacata gcgaggcgcc cagcgccgcg aacaccaggg
    11341 agctaccgag ccggctccac gatcttttcg tagtccagct ccacttcgcc gcctcggcag
    11401 accgtttcat gattcggaca gtctacaccc gaaacgttat aaaatgggct gccgcgcacg
    11461 tgtaaccgat cgcgcgtacc cctttataag aatcgacatg acacgacgca gttttttctt
    11521 ctcaaagatc gccggcgtca ctgctctcca cgccgcaaca ttcgctcgcg ctgccgagcc
    11581 ggccacccca gtctccctgc cgcgggcgaa gtggatcgag cagggcatca tcgacgccgg
    11641 cggctcacac gagccgtact ccttcgtggt tcgccgtggc gggcaccggc tggacgccta
    11701 ccggcgttat caacagacac agagcgaaga ggtcatccgc cgcctcaagg acaacggagt
    11761 cgaagtcttc cacacccatt tctacaaggg cgcgggaatg gttcatgaaa aggccgagat
    11821 ggaagatgca aaacgcgtcg cggagatcgc gcatcgatac ggcctgaaag ttgactcgta
    11881 cctccagtgg aacacgatgc tgtacgagac attcttcgcc gaagagcccc gcgcgaaaga
    11941 ctggatccag cgcgacgccg acggcaggcc gatcctgttg cggtacggcc accagcagtc
    12001 cttccggtac cgcccgtgct tctccaaccc ggaatacctt gagtatctga agaagcttgt
    12061 gcggtacgcg gtcgaagagg tcaagaccga cttcattcac ttcgacaact acgacctcag
    12121 cccggagccc gactcctgtc actgcccgaa ctgtgtgcgc ggcttccgcg actttctgcg
    12181 ccgcaagtac acccccaaga ggcggaagga gcggttcggg ttcgacaacg tcgactacgt
    12241 gaatccgccg cgctggaatc gcccgaatcc tcccgagaag atgcagatca tctacgatcc
    12301 gggcatacaa gagtggatcg actaccgctg ccactcgatg gcggaggccc tccggcagat
    12361 ggcggaatat gcgaagtcga tgaacccgga agtggcgatc gaagtgaatc cccacggaat
    12421 cacaggcggc aaccgggcgt gggaggcggg actcgatcac gcccgcttcc tgaactacac
    12481 ggaagtgttc tggacggagg aggacaacaa gccggcgtac cggccggatg gccggctcac
    12541 gtccacaatc cgcagctaca agcttgcccg agccttccgc aacgttctgc tcacatatat
    12601 ctcgggagat ccggcatcga tggccgagtg cctgtcgttc aaccagacga tcggcttcgc
    12661 gggcagcgat cctatcgcgc cggagatgct gaaatacatc gccttctatc gcaagcaccg
    12721 ggatctctat gtcggggcac aggacgccgg cacggtcgca gttctgcgtt cgtttgcctc
    12781 aatcacttac aaccaaccgc gggttcagct tggaaccatc ctgatggagc aggcactcat
    12841 tcaggcgagg attccctttg atctggtttt cgatcagcac ctgaaagacc tctcgaagta
    12901 tcgcgttctg attcttcccg agaccgagtg cctgagcgac gaacaactcg cgctggttcg
    12961 ccggtttgtc gaaaacggcg gcgggctgat cgctactggg caagcgggca tgtacgacga
    13021 atggcggcgc gtgcgtgtgc agcccggttt ggaagggttg atcaatatca aagcagaagc
    13081 ggtcggctac caggagagag tcgaacaaac tgaaatctcc ggagccggcg ttcgcaagga
    13141 agtcgcgaag ggtcgtgtcg tctacttccc cgccgtccag tttgacggcc cgctccccga
    13201 acatgaggac tacttcgaga tcgggagccg gttctggaaa ctcccgaaga actggatgga
    13261 gatcacagag agcgtccggt gggcggctcg ggaggagatc ccggccacaa ttaccgggcc
    13321 gctgcacctc gtcgctaacg tcgtcgatca gccggagaag cgcaggcgca tgattcacct
    13381 ggtcaattac aacagcggga aagttcccga ggtcgcgggt gtccgtgtga cctgccggtt
    13441 gccgcacggc gccgctccca aagaggtcac cctctactcg ccggatctga acgcaccgcg
    13501 cacgcttcct accgccaaca ccatcgccgt gccaccggtc aaagtgtact cggtcgtggc
    13561 ggtgagttgg taacgacgcc ttctactgca ccggcgtctg cttgagccag cgctcaaaga
    13621 acgattgcca agccgggtcg atacccgggc cagtggcgtt gtgtccgtct ccggtgtgcg
    13681 cgaactggat gcgctccggg attcccagaa gctggtagac ttcccgtgcc cggttgaagt
    13741 acccccagct ttgcagatcg tcggaatcgc cgccgttatc ctcgcgttga ttcatgcccc
    13801 cgatcagaag aaacgctcgc ggtgcgacca gcgccatcaa ttcatggtgg tcgtgctcga
    13861 aacccggccg cttcgggttg gggttcagca tgcttaacgg cgtgcgctcc gcagtcggta
    13921 tgcccgggaa cggacgcagc cagtcgagat accacggcgc gtaccagttg gttcctccat
    13981 ttacggcgat gtgggggtcg agcgccaccg tagccttaaa ctcgggtgcg aacgcggcca
    14041 cgtagaccgc cgccttggcg cccagcgaaa aaccgatgaa tcccaaacgt ctcccgtcga
    14101 cctgcttcag gctgcggagg tattcggcct cgcggcgggc gtcgtggacc atcttgccga
    14161 gtggcagcca gtgcccgaat cgctcgtata acttctccgg cacacccttt ccggcgctcg
    14221 ttccgtcgcg atagttgcgg atgaacgacc atccggtcag cacgacgtaa ccgcggcccg
    14281 cgagccagga tccccaccac tcttccggtt tctgccagtc aggcgaggag gacgtccagc
    14341 agattactgc cgggaaaggt cctggtccct ggtttttcgg cagcagcagg agatgcggtt
    14401 gccagaaatc cttctccatc ggcaactcga gatggatccg cgtgtacgcc gggcgctcct
    14461 gcttatcgat gatgcgcgcc ttgcgaatgt cgccgaacca gcgcttgtcg gggcccttcg
    14521 gttcgagctt gccgagcatt gtggtccagg acttcaggat ctccgggcga cgcttcttgt
    14581 accagtcctt cggagtgtgc aggtcctcca caagggacgg aatgatttcc ttctctgcgc
    14641 cgggctcggg cgccgggctt cttttctgac tgacgcggtg ttggttgaga cgctgagttg
    14701 ggtcgggggc ctgcagcgga gcagccgtca gagcgagcag cagaacgagt atgagcacta
    14761 agcacctgcc tctttcttga atgttgatga tgagaccggc aagcccgacg cctcccacgc
    14821 agcgattccg cccgtgaggt ccgccgtttg ctgaaatcca tgccgctcca gaatgctggc
    14881 cgcgagcgat gaccgatacc cgccctggca gtgaaccaca accagccggt cacgcggcag
    14941 ttctgcgaga cgttcgggaa ggtgattgag cgggatgttc ctcgagccgt tgatgtgctt
    15001 ctgttcccac tctcgcgggg tccgcacgtc cagaaccacc ggaggtttcg cggattgaag
    15061 ctgttcggcg agggtggccg tggcgatccg ggcggttcgg ttaagagggc cgggcagcgc
    15121 ttcgaaggcc tgcatgccac catgcaagca gcctgtgacg ttgtcgaaac caatacgacc
    15181 aagccgcgtc gcagcttcga gttcggagcc cggatcggcg atgatcacga tcggcttctc
    15241 gcggtcgagc agggtcccag cccaggtcgc gtactgacca cccaaaccga tgttgatgct
    15301 gccgttcagg tgcgcgccgg cgaaatctgc tggatttcgg acatcgagaa gctgcgctcc
    15361 cccgcttcga aggtgcaaga gttcctcggg cgacagcggt ttcaggacct gctggagtgt
    15421 cttgtcgagg gtcggccgtt ctttcgtgtt cagaacagcg tcgtaggtga aatatgccgg
    15481 tgcttcgggc tggtcggcgg ccacgatact gacaaactgt tcccggctca tcggttgcag
    15541 ggcgtagttg taccggcgct gggttccgat ggtggagacc gtctccgtgc cgagattctt
    15601 cccgcacatg ctgccggcgc cgtgtccggg gtaaatcagg gtttcgtcgg gtagcgtaag
    15661 cagcttgttc cggaccgaat cgtaaagcat ccggccgagt tcctctgcag tccacccgag
    15721 cgacgcccgc aggtccggcc ggcccacatc gccgataaac agcgtatcgc ccgtcagcac
    15781 cgcgtgcggg cgctccgtgt ccttcgccag atcatgaacg agaatgcaga tcgattcggg
    15841 agagtgtccc ggtgtctcca agacagtcag ccgcgtcttg ccaaactcga tcgcgttgcc
    15901 atcggccagt gccgtgaagt catactcagc tttcgcgcga gcgccaaggc agatcgtggc
    15961 gccggcgagg tctcggagtt ccagatgacc agccacaaag tccgcgtgaa agtgagtgag
    16021 aaacacgtag cggatggcca agtccagctg gcgtgcatct tcgacgtact cgtcgacgtc
    16081 ccgccgcgga tccaccaccg cggcggtgtg cgattcttcg tccgccacga gataagaggc
    16141 gtgcgcaaga cagccgaggt agtactgctt gagaatcatc cgaacctcct tgcccagtag
    16201 gagccggcac ccgcccgacg gggaccggga tcatcctcaa tccttgatcc aggtgtgcct
    16261 tggatcgtcg ctccaggtgc cgtacgcacg gccctcgccg gccagcaccc aggtgtagtt
    16321 cagagtatag ccgggacagg cgaccacggg gtggtatccg cgcggaatca gcacggtgtc
    16381 accatgctcg accacgaacg cgtgatcgaa cggttccggg tcatcagcgc cggtgtacac
    16441 gcggatgaag ccgaagccct ggttcggctc gaacaggaag tagtagacct cctccatcgg
    16501 cacctcaccg gcggcggtga accggtcgtg tttgtgcggc ggagagctcg tccagccgcc
    16561 cggttggctg accgtctcgc cacaaatcag gtgtacagcg tccacgttgt cggcgatgtg
    16621 ggtgcagacc gtacgggcga agttgttctt gccggtgggt gtcaccatga tctccgaacc
    16681 tgcaatcagc cgcggcgacg cgcccgtctt cccggcggcg ccggcggctg ttacgcgcgc
    16741 acgctcgtcg agacgggata tcttggcttt cgaaccggca ggcaggtaga tcatcggccc
    16801 ggtctccttc agggttcgcc tggcgcctat ctccgccgac cactttcccg aagagttctc
    16861 gacctccacc cgcacaggac cggcgtagga gtcgagcgac tgctcctcct ctcccgtctc
    16921 ccactcgtga gttcgcaacg ccccgcccag ctcgatgatg gtgaaggaga ggtgtttcag
    16981 tccttcaccg cgtttgacga tcggataaac gccgggcgca tctccaggcg acttgatatg
    17041 aagacccata ggcttcacgc tatcacacgc gtttgaggcc ctgaccgagg tgtctcaaag
    17101 catggtgaaa cccggagtgc ccttcagcgc acggtcgaaa gtgactgtgt ccgggcaacc
    17161 cgaagcgcgg ctaatctcac caatgaggta gtcagagaaa tcggccttcc cgttccggta
    17221 accttccaga ctccggcgca ccagggcgtc gtgctcgatc ttgaactgtt cggtctgaag
    17281 gatgcgctca agtatcgcga cgatctcggc tttcgactga tcgtagcagc ggaacaaaac
    17341 ccaaaccagc ttacagagaa ccagggccgg gaggaagatg aactcaccat cctggttgca
    17401 tcggtcgatg aacctcgagg cagccacaag ttgcctcgag tcatcggccg tcaggtaacg
    17461 cacgaggacg ttggtgtcca gcgccctcat gccttcgagt acccttccgc aatcgcctca
    17521 ttcatttccc gaaccgtaac ggctcgtttt cgggccggcc gcacgatgcc gcgaagcccg
    17581 cgtacgtcaa gagtgcgagg cttcatggtc acttgcccag ccaagtcgaa ctggaattcg
    17641 attcgttgcc cactccgcaa ccgaagccgg tcccgcactt cttttggaat ggtgatctgt
    17701 cctttactcg tgagggtggc ggtgagcatc tccttactcc aatctgattc cttacttaat
    17761 ggtaatccgt gatctcgtca ggaatcaatg gcgatgcagg atcgtggcgc tggcgctaag
    17821 cttccggttc acctcatccg ctccggcacg ctatcatgga gccattgtcg cgatgcgctt
    17881 cctcgtgtgt ttcctcttgc tggcgctcgg tgcgccggcc cagcaactga ctctggaccg
    17941 gcttttccgg cggcccttcc tgtggggcac gtcgccggaa caggtgactt ggtccaaaga
    18001 aggacacact ctcgccttcc tgtggaacac ctcagggaga cgcttcctcg acctctatgc
    18061 gtatcatccg ggcagcagaa agctcacgcg gcttaccgat ctcgaaccgg tcaaagacga
    18121 tctcaacctc acggaggagg agaaggacga ccggcagaag agttaccttg agccgctcgc
    18181 gggcctcggt tccttcgaac tctctcggga tagttcgcac gccgcgttct cgtaccgtgg
    18241 cgacctttac gtggtcgaga cccaaggcga gaagccgccg ttccggctga caaggaccaa
    18301 ggcttcggaa tcctcgccgc agctctctcc ggacaacacg agaatctctt ttctccgcga
    18361 tcggcaactg ttcgtgcaag acctacgcga cggccggctc tggcaggtga ccgatatcga
    18421 ggagcctgat caggcgatca cctcgtatcg ctggtcgccg gatgggacgc ggttcgtcgt
    18481 cgcggtgcgc aagggccgcg gacggcagat ggtgcttccg aactactcgg gccgcttcgt
    18541 caaggcgggc tctttcagcc gcagcgtggc gggcgatgag ccggtcgaaa cggcgttgtc
    18601 tatcgttccg gtggagggcg gcaagccgct gcccgttgac ctcggccctc tcggtgccaa
    18661 agtctatctg ggtctggtcg actggtcgcc cgactccagc cagttgctgg ttcgtagcgt
    18721 tcacccgaag gtgaagcagg cgcacctgct cgttatcgat gcgaagaccg gcaaatcggt
    18781 cgttctccag gaggagacgg acagcaaatg ggtccactgg acctcggctg catggtcacc
    18841 ggattctcgg aatgtcgtgt tcacgagcga ccgggagggc tgggcgcagc tctaccgaat
    18901 tccggctact ggggggaagc ccgagcagtt gacccgcggc gcctgggaag cgcaggaccc
    18961 gcagtgggcc ggcgagaata tctactacac gtcgactgag ggcggtgcgg cggagcggca
    19021 cttctaccgg atccgcccgg atgggtccgg caagcagaag ttgtcgcgac gggaaggatt
    19081 gaactacggc ctcgtctccg aagacggtat gcacgtcgca tggctgcaag cgaatctgtc
    19141 gaatccatct gacctctacg tggacgagga tcgggttacc acttcacccc tgccggaatt
    19201 cgccaactac cgctggccgg agtcgcggtt cttccagtac ccgtcccggc gcgatgggaa
    19261 aagtgtggcc gctcggctga tgctgccgcc tggatatcgg caggacgaca aaagcgggaa
    19321 gcgttggccc gccgtcgtct acatccacgg ctcgggttac gccaccagcg ttttgaaaga
    19381 gtggggcagc tacgaccctt accggttcgc tttcaatacg tatctcgccc agcgaggctt
    19441 cgtcgtgctg gaaccggact accgcggcag ttccggttat gggcgcgatt ggcgcacgga
    19501 tgtgcatctc catatgggag gcccggatct cgatgatgtc ctgggcggcc ttgactatct
    19561 ccgtggtctc ggcgacattg acatgaagcg catcggtatc tggggtgtca gctacggcgg
    19621 ctttatgacc aacatggcga tgttcctcgc tacggatgag ttccgcgcgg gagtatcctg
    19681 ggcgggcgtc aacgattggg aaaactacaa cgcctggtac acctccgagc ggctgactac
    19741 cccagaggag aatcccgaag cgttccggcg cagctctccg atccattttt cgcgcctgct
    19801 gaagaacccc ttgctgatcg tgcacggcat ggtggacgac aacgtgctct tccaggacgc
    19861 cgtacagctc acggaaaagc tggttcagga gggccggccc ttctggcaca tctattaccc
    19921 gcaagaggac cacgcctttg ttcgcgacga aacgctcgtc gacgcctaca gccggtccgc
    19981 ggagtggttc gaaaggcatc tgcgatgaag ctcccacggc tctatccgat cctcgatacg
    20041 gctactttgg aaaagcaggc atgctccata gaattggcag ccgaagcgat ggtcgccggg
    20101 ggagcgagga tacttcagtt ccgccacaag ggtcacttct cgcgggcgtt gttcgagcag
    20161 gcggaacggg tcgcggcgtt gtgccggcgg gagggagcca ttttcgtcgt ggacgatcgc
    20221 gccgacgttg cgatgatgct cgacgccggt ttgcacgtcg gccaggacga cctgccgcct
    20281 tctgacgcgc gacggctcgt cggcacggaa aaactgcttg ggttttccac acacaatgag
    20341 gttcagctcc gggctgccgc cgccgagccg attgattatg tcgcactggg accggtcttc
    20401 ggaacggcgt cgaaagatcg tccggacccc gtggtcggtg tcgaggggtt gcggcggctg
    20461 cggccgctcg ccacccggcc actggtggca attgggggaa ttacgcgcga gaatgcactg
    20521 acggtgctcg acgcgggcgc cgactcggta gcggtaattg gggacctgct gcccgagaag
    20581 tgcactttcg aaactctgag aactcggatg gggctatggc aacaactcgt gacgacgtaa
    20641 acacagggct cgtcaaagga ctcgggctgc tcgatgcaac caccattgtc atggggtcca
    20701 tgatcggctc gggcatcttc attgtttcgg cggatatggc ccggcagacg gcctcgcccg
    20761 gcctgttgat cctgacctgg gtcatctccg cggtcctcac gatcatcgcc gccctcagct
    20821 acggtgaact cgctgcgatg atgccacagg cgggaggaca gtatgtctac ctccgcgagg
    20881 cgttcgggcc tttgttcgga ttcctctatg gctggacgct gttcctggta atccagaccg
    20941 ggaccgttgc cgccgtggcg gtggcatttg ccaagttcac gggcgtcttt ttcccttggt
    21001 tttccgcagg caacgcgctg gtccgctttg gcgggttcac gatcaataca cagcaggcgc
    21061 tcgccatcgc cgtgctcgtg ttcctcactt gggtgaacac ccgaggcttg cgcaccggag
    21121 cagccgtcca aaacgtgttc accgtcgcca agaccggcgc cgtgcttggt ctgatcgggc
    21181 tcggattcct tgttggcggc gccggcggct caggcaactt cgagggattc tggcgcaacg
    21241 cgggctggaa cttcgacgcg gttcgcatcg ttggtgtcgc gatggtcgga gctctcttcg
    21301 ccgccgacgc ctggaacaac atcacgttca ccgctggcga ggtacgaaat ccacgccgcg
    21361 acgtgccgct cgcgctgggc atgggcgtcg gtgtggtctg ccttctttac atcgcctgca
    21421 acttcgttta tctgcgcctg ctgccgctcg aggcgatcca gacggccgcc gaggatcgcg
    21481 tggcgaccgc cgccgcgtcg atgatcttcg gcccggtggc cgtgcaggtg atggcggcgg
    21541 cgatcatgat ctcgacgttc ggctgcatca atgggatggt cctggctggc gcgcgcgtct
    21601 actacgccat ggcgctggac ggcctgttct tcaaggcggt agcgaacgtg gacccagtcc
    21661 atcacacgcc cgtggtctcg ctgaccgtgc agtgcgtctg ggcctgcctg ctcacgctgt
    21721 cgggaacgta cagcaacctg ctcgactacg tcatcttcgc ggtgctgctg ttctacattc
    21781 tgaccatcgc cggcgtcttc gtgctgcggc gcaagcgccc cgacgcgccc cggccgtaca
    21841 aggcgttcgg ctacccggta cttcccgcgc tatacatcgt ccttgccggt gtggtcgaag
    21901 tcctgctgct gctctacaag ccgaagtaca cgtggcccgg actcgctatt gtgttgctcg
    21961 gcctgccggt gtacttcgtt tggcggcgga ggcggacctc agcgctttcg tgagatccgc
    22021 gctgagcacc gtcgggcacc agtatttttc gatgtgctcg attaccagtt cagtgccgcg
    22081 ggcatgactg acgaagggac tcttcttcgg gtcgtacatt gcgtcggtca ggtcgcgcac
    22141 aagcacgcag cgcactcccc atttcgtcat ctgcttgatc gcaaatgtac ggttgagtac
    22201 gcacatgttg gtgtgtacgc ccataatcaa caaattgccg atcccgttgg cgcgaaggta
    22261 actgtacact tcggttcctt tgtctgagat gacgtcctcg ggcgcaatgc gaatgccggc
    22321 gtgctgccgg gtccacgctt tgtgatcctg gtcgccggtg tcgcatccgc cctctttgtc
    22381 gtcgatcgga agcggcggat cggagaatgt catcggctcc gggggcaaca cctgtggcag
    22441 ttcgagcatc cgcttccgct gcgggtcgtc cttgtagaaa tccatgacat ccgatgggga
    22501 gtgaatgatg cggatcccgc ccgtgcgggc tgcctccacc accggcgcca tcctcttcac
    22561 catctcgttc actcgctgcg cggcgcctct gcaccaatgg ttgtcccaca tatcgcagat
    22621 gaggacggcg gtctgaggca cagcgaggct tttctcgaac cggacttcct cccattgcgc
    22681 gctgcccttg aacgcctcga ctcgggagcg aagctgtagc cgcagttccg gctggcagaa
    22741 tgcgggcaga gccgtgagaa cggcgatgag cacgctggat ctgagcatgg agcgattatg
    22801 atatatttcg tttcttgttt cgctgggtac cgacaacgaa aggacactgg tgacctcatg
    22861 agcctttggg cgaccaaacc gctgagccgt atcatggctg agagcgaagg cggcgagcac
    22921 actctgaagc ggacgctcgg ggcgatgagc ttggtcgcat tgggcatcgg agcgattatc
    22981 ggagccgggt tgttctctct caccgggatt gccgccgccg agaatgcagg accggccatc
    23041 gcattgtcct tcatcgtcgc ggccatcggc tgcgcgttcg ccggcctctg ttacagcgag
    23101 ttctcgacca tgatcccgat cgcgggtagc gcatatacct atgcgtacgc gactatgggc
    23161 gaatttctgg cctggattat agggtgggac ctggtcctcg agtacgcggt cggcgcggca
    23221 accgtctcca tcagttggtc tgcctacatt gtgagcctgc tgcacgacat cggcatcaac
    23281 tgtccgccct acctggttgc ctcgccgtgg cagcccgttc agcttccgaa cggccagctc
    23341 gtgcatgggc tcatcaacct gcccgccgtc ttcatcattg ctctcgtctc cacgttgctg
    23401 gtcgtcggaa tcaaggagtc ggcgaacgta aatgccgtca tggtgatgat caaggtcgcg
    23461 gttgtgatca tcttcatcgg cgtgggcatc tggttcatca agggatccaa ctatgtcccc
    23521 ttcattccgg aaaacactgg cacgtttgga gaatatggct ggagcggcat cctgcgggcg
    23581 gcgggtgtga tctttttcgc atacatcggt ttcgacgccg tctcgacggc ggcgcaagag
    23641 gcgaagaatc cgcagaagga catgccgatc ggtatcatcg gctcgttgct gatatgcacg
    23701 gtcctctacg tggccttcgc cttcgtcatg accggactcg cgcactacac cgagttcaag
    23761 ggtgcggctg ccccggtggc agtggcgatc gccaagaccc cctacatgtg gctgggctcg
    23821 ctggttaagt tcgccatcat cgccggcctg acttccgtaa tcctggtgat gctcctcggc
    23881 cagtcgcgtg ttttcttctc catgtcgcgg gacgggctgc ttccaggagt cttttccgag
    23941 atacacccga aattccgaac gccctggaag tcgaacctgc ttctgatgat cttcgttagc
    24001 ctgttctcgg cattcgcgcc catctcggca gtcggccaca tgacgagcat cggtacgctc
    24061 tttgccttcg tcatcgtttg cgtcggcatc atcatcatgc ggaggaccca gcccggtttg
    24121 ccacggccct ttagaactcc gtgggtgcca gtggttccgg ttctcggcat cctggtcaac
    24181 gcgatgatga tgtacggcct cggctgggag aactggatgc gcctcttcgt ctggctggcg
    24241 atcggactga cgatctactt cctctacggc cgcgtgcaca gccacctggc ccggaagtag
    24301 ccaggacgtt tgtcgaagct cgaagacctc gtctgcactc cgggctgggt ctcgcgctaa
    24361 actgctagtc catggacacg ctcatgcctc tggcgtccgc gctcggcttg ggtatgctgg
    24421 cgggcgcgcg cctctatgcg accgtgttcg tcgtcggcct gttgctccgg ctggactgga
    24481 tcgccgtgcc cacggcctgg cagcacgcat cagtgcttgc ggatacgcgg gtgctcgtcg
    24541 cttccgggat cgcttgcgcg cttgaattca tcgccgacaa gattccctgg gtcgacagcg
    24601 cctgggactc catccatacg tttctgcgcc cgatcggcgc cgctctgctg acctcctcgc
    24661 tgttctcgaa tctggagccg gcatatcagg tcctgttgtt tctgctggcg ggcggagtcg
    24721 cgttctccgg acattcggcc aaggccgcca cccgattcgt ggtgaatcat agcccggagc
    24781 ctttcagcaa cgtcgcggtc agtctcgccg aagatgcttt cgtggcgggc gggctttaca
    24841 tgcttgtgaa gcacccgtgg atcatggcgg gcatcgtgct tctgttcctc gcgctgttcg
    24901 cctggctggc tccacgagtt ttccgtgcgc ttcgggcgga gtgggccgcc ctgaccgccg
    24961 tgatacggaa ttggacgggc gacagcgtgc agccgtcgct cgctccagcg gagcgcgagt
    25021 ggctggcagc gaatcacccc ggccggcagc ctggcgaggt cttttgcgtg atcgccacga
    25081 gcgatctgaa aggcctccgc aacgccatcg ggacactctg cctcattgga ggcgatgcgg
    25141 cgttcttcgc tcggcggtgg atgcgcccgg tccgcaagac gatcggcacg gtgacgggca
    25201 cggagacgcg gcagggtatt ctcgtagacc ggctgatttt ggcgctcgcg gatggatccc
    25261 gcatccgctt cgaccttctg gctggacaga tgccgaaagc tgcgcagttc gtgcagccgg
    25321 ccgcgtgagt cgcttcccgg ctatttcctc cacagtgccg cgccgccgag caatccaagg
    25381 acattgggga cgactttcac gttcggaggc agcccctgga gcgaaacctt cttcgcgttg
    25441 ccgccgccaa gatacagcca gtcgtagttg aagaggtgct cgagctcggc gaccgctctg
    25501 gcgagcatcc ggttccagtg cttcacgcca agttcgtcgt agcctgcctt tccgagccat
    25561 tcctcgtaag taagcccttt gtggaacgga tggtgcgcca cctcaaggtt cggcacgagt
    25621 cgcccttcga gaaacaatgc ggatccgagc cctgttccga gagtaagcac gagttccacg
    25681 cctcgcccgc gtacggcgcc cattccctgg atgtcggcgt cgttggccac gcgagccggc
    25741 ttgtgaagcc gctttcgcaa cgccgctgcg atatcgaagc cgacccagcg gtcgtgcagg
    25801 ttgtaggccg tgtaggccac gcccttgcga atcacccctg gaaaaccgag ggagatccgg
    25861 tcgaacgggc gctgggccgc agccaacctg tcaatcgtac gcaggatggc cgcgggcgtc
    25921 gcgggcctcg gcgtttccaa gcgcgtccgc tcggtgagag gcctcccttt ctggtcgatc
    25981 acaatcgctt ttaccccgct tcctccgatg tcgatggaga gaattcgaag cggcgcggtt
    26041 cccgtctgtg tcatggcaat ccttggctgg tgttctccgg atgatacccc agagtgcggc
    26101 aagcgggtgc ggcttgattc ggctccagag gcttcgtcac gcggaaagcg cggcggttga
    26161 aatgaactgc ttcagatagt tcgcactttg ccggagcgcg gcctgccggt tttcgagaga
    26221 gccctcgaag gcgcggtctt ccacctcgac gcagaccggc ccatcgtatc cggtctcggt
    26281 gagcacggag aagaaacggc cccaattcac gtcaccgagc cccggaagct tcggcgtatg
    26341 gtactgaagc gggtacgcga gaattccaac ttcgtcgagt cgctcctggt caatgcgggc
    26401 gtccttggcg tggacgtgga agatacggct ggcgaactcg cgcaacggct tcagatagtc
    26461 catgcgttgc cagatcatat gggatgggtc gaagttgagg ccaaagttgt tgtctggaat
    26521 gtcgttgaac atccgccgcc aaatggccgg cgagacggcg agattcttgc cgccgggcca
    26581 ttcgtcggcg ctgaacgaca tcgggcaatt ctcgatccca atcctgatcc cgttgtcggc
    26641 ggcaagccgg atcagcggct tccacacttc gagaaaacgc ggccagttgg cgtccaccga
    26701 tttcgtccag tcccggccga tgaaggtatt gactacgcct acgccgagca ggcgagcgcc
    26761 caggatcact tgcttcagat gatcgatata cacctgggcc tcggcggagt ccggtacaag
    26821 cggattcggg tagtaaccga ggccgctgat ggcaacgcct gctgtcttca cggcgtcgtt
    26881 gatacgagcg gcgtctgcgg cggtcaagcc cacgacgtcg atgtgcgtga cgcctgcgta
    26941 gcgccgctcg gccttaccct tcggccagca catgacctcg acacaagagt agccgctggc
    27001 ggacgcaaac gacgcgactt cccggagcgg caggtcaggc aaaatggcgg tcacaaatcc
    27061 gagcttcagc atgatgtctt tctcccgggc gcgtgttccg cgccgaagat ccatcttaga
    27121 atggccgtac gcccatttgg agcggacttg tgcattgcta gagtttcatg caaagtaaag
    27181 tggggattgc aatgaataaa acccgatttt tcgtgaccgt tcccgttgca tctgttctgc
    27241 tcgccctgac ggcttgctca agtccgccac cgcaggaaaa agctgccgag gtcaagaaat
    27301 ccccggggcc ggcggctaac gccggtgcat cgcagctaac acgcgacccg ctgccgggct
    27361 gcgagtggcg aggatcggtc gatccgagta tccgtctcta catactcgtc cagcaatgca
    27421 cggacgccgc tcacaaaaat tcgttcctgg cctatgggaa cgccatctat cagatacccg
    27481 ccgccgccgg cggcgagatc gcaaagggta aacgaatcgt cgaagtgttc gaaaagcctg
    27541 ccggccagcc cattgaacaa gcgctccagg agcagttcat cgctaagctg ccgccgcccg
    27601 gaaaagaagg gtgcctcgtg cgttcaccgg ccgagggcgt ggtggtttcc gacccgacta
    27661 aggtgaccta tgagatcgtg ccggggccgg aatacaaagg gggatcggaa gcgggctcgc
    27721 gcccctgcgg cgaatacgga aaggccgact cgcccatgta tttcgagtac catccccgcg
    27781 agtccagggt ccgatttctc tttgtaaacc ccagcggcga cccgccgatg ttcgacgccc
    27841 aggcgatccg gttacggtag catccgccac cgggcagcga accgcaactt caccggttcg
    27901 tctacgacga tcagccaacc gcgtccggga gtaacccgca ccggggttcg tctcacggtt
    27961 ccgccgtaag gcacttcttg cgccgcaccg gtatattact cccgccaatg catggattag
    28021 aattggacca gggaagactg gaagcggccc agtcgccatc tagcgacccg gttccgcgcg
    28081 ttgccgtcgt cccttggtca accgggttca actcccgacg aatccgcccc gcggggccgg
    28141 cggaaacgca acaaggcagc ggccttgcag gttaagacct ccgccgctgt tttgtggccc
    28201 ctcggggtgg atcatgattg gcaggaatac tgatgataaa catacttcta gcgctcgccg
    28261 tccttgttgc cgtatctgtt gcctggtcca ggctgcgttt caagccgatc acagttttcg
    28321 aatacgagcg tggcctgaaa taccagaaag gcagattcga aacagtcctt cagcccgggc
    28381 ggtactgggt ctacctgccg cacacgtctc tggtgaaact cgacatcagg ccgcgcttcg
    28441 taaccgtccc tggccaggaa ctggtgagtt ccgatggtgt cagcgtgaag gtaagcctcg
    28501 ctgccaggta cgaggtcgcg gacgcttacg tagccgtcct gaaaaacgag aactacgaaa
    28561 cggccctcta tcttctgctg caagtcaagc tcagggagat cgtcggagcg tcgaaaatcg
    28621 acgaactgct cgagaaaaga aacttgctgg gtcagatgct gatggaatcg tccgagaagc
    28681 ccgccgagga gctagggctg aagctgcatt ccgtcaatct gaaggacatc atgtttccag
    28741 gcgaattgaa gaaggttttc gcgcaggcag tgaaagctca aaaggaaggg ctcgccgccc
    28801 tcgagaaggc acgcggtgaa accgcagccc tgcgaaacct tgccaacgcc gcgaggatga
    28861 ttcaggaaaa cccggcgctg ctccagatcc ggttgttgca gcagttgggc gagacgtccg
    28921 gcaataccgt ggtcctcgga gttccggcga cgacgccgct gcctatacga agcccccagc
    28981 cgtctagacc cgacaccgcc caactggggg cttcggaaga aacaaatgaa tagtcgccgg
    29041 atcgcctctt acctgtctta cctgcttcta taacgaacga gacctcggta agacttagcg
    29101 ctgctcaatt ccctccgata gactgggttc ttaggccaag ccatgaacaa cagcacctac
    29161 actcacatcg ccgacctgat caaggaggtc gagattccgg tcaacggcat cctcagccgg
    29221 acgctttaca acgacgatcg tttgaaggtg gtcatcttcg gattcgccgc cgggcaggaa
    29281 ctgaccgctc acaccgcgcc catgccggcg gtgattcaga tcctgagcgg cgaggctcgg
    29341 ctcaaactgg gcgacgatac gatcgaggcc ggacctggga cgttcgtccg catgccgccg
    29401 caactggaac acggcgtcta cgcgacgtcg ccagtggtga tgctgttact catgatgaaa
    29461 aatgcctgag cgcaacgcca gaaccccccg cagccgggct gtgaacgcac gatgaagatc
    29521 cccaagttgc gctggatcat cgccgccatg ctgctgctcg cgacgatgat caactacgcc
    29581 gacagactgg ctctgtccgt cgtctcagtg gatctgcgca gcgagttcgg cctcgacgag
    29641 caggactacg cgcagatcgt tgccctgttt ttcttcgcct acgccattat gtacgccggg
    29701 tccggctaca ttgtggaccg gctcggcacg cgccgcgggt tctccgtatt catcttcggc
    29761 tggtctctgg ctcagatgat gcacggcttc gcccgcggaa agtggtccct cgcaggttgc
    29821 cgcttcatgc tcgggctggc ggaacctggc aactggccgg ctgccgcgaa agccgtggcg
    29881 gagtggttcc ccgcctcgca gcgcgccctg ggtgtcggga tcttcaatgc gggctcgtcg
    29941 ctcggctccg ccatagcgcc gccgatggtc gcctggctga ccctgatgta cggatggcgt
    30001 tccgccttca tctttacggg tacgctgggc cttgtctggc tcaccgcgtg gcttctgctg
    30061 taccagccgc ctcaccggaa ccggtggctg cgcgagaaag aatatcttga gctacgagat
    30121 caggtaaggc cgcccgagga gaccctcccc gcctcccggt ccgccctcga ctggcggaag
    30181 gtgatcacga tgcgcggatg ctggacgctc atcgtcgcgc gctttttcac cgatcccgtg
    30241 atctacttcg ttatattctg gctgccggaa tacttgaggc gcgagcgagg tttcgacctc
    30301 gcgatggtcg gcaggtacgc ctgggtgccc ttcatcttcg gcgacatcgg ctacgtgctc
    30361 ggcggttggc tctcgggccg tctgatcaag gccggttgga agctccccac ggcgcggaaa
    30421 ttcgtgatgc tcctcggcgc ggccgtgatg ccggcggcga tgttcgcgcc tctggtcccg
    30481 gccgcctgga tggcgatcgc ggcgacctgc ttcgtgactt tcggacacgc gttctgggtc
    30541 gcaaacctgc aaacactgcc gaccgacctc tttccgggtc acgaggtcgg caccgcgaca
    30601 ggattctccg ggatgggcgg agccatcggc ggcgttcttg ccaaccttgg caccggatgg
    30661 gtggtccagc acttctccta taccccgatc tttcttctcg ccgggctgat gcaccccctg
    30721 tcaattgtcc tcgtctactg gctgctgcct tcgcggttct ttcggaaagc cgagatcgat
    30781 ccaactccca taagtggtta acaataaata tcttcccaac cctatgatgg gctcctaccc
    30841 cgattctgcg ggtgtttttc ggaaacaaaa gtactacaaa aggggaaaat ctcctgtccg
    30901 aaagatagcg tgacgcgagc ggcgtcctgt gcgtaaataa aggctcggga caaacatgcg
    30961 aggcgctgat cgacggaacg gcatcaggtt tccactcaaa ctgaactgcc gggttgtctt
    31021 tccatttaaa gcgcggtgca atttcgaagg tattaccgag aatctgagtc gagataacgt
    31081 ccttattcgc gtccccaggg aaagaattcg gggcacgccg ctccgtgtcg gagactgggt
    31141 tcggatcatg ctggatctgc ccggaacgga gagcttgccg tctcggtgcc tcggctgcga
    31201 ggctaccctc gcccgagtcg aggacgacgg acccgaagcc gtccacctgg ccttccagat
    31261 cgatcagatg gaattctgcg agagtgagaa cggcgaggcc gccggccgcg ccagggtctc
    31321 ccagatcgcc ctcagcgggc tcgcgacctg aaagcgcggc tggatcagat ctggtcctga
    31381 tagtcctcta acatcacccg caccaattgg ccgcgcgtgt gcactccggt tctctgaaac
    31441 agccgctgca ggaccgtttt tatgatcgtc tcggagaccc ggaggcgccc ggcgatctcc
    31501 ttgttgctga gcccttcgaa caaggcgcgc accaccttcc gctcccgctc ggagaggtgc
    31561 ttgggcaact cagccgaatc cgagcccgcc tcaacgcccg tcaccagttt cagaaagcgt
    31621 tgatccagcc aggctccacc tcgcgccacg tccacaacgg cttccaccag cgccgatgtc
    31681 gggttttcct tgggaaaaat gcctgccacg cccgaacgca tcagacggac cgcctcctga
    31741 tcgctgatcc atgccgttac cacgagcaca cgaccctcaa agcccaggtc ccgcgccgcg
    31801 ggtaggaacc gggatccacg ctcagagccc aggtcatagt cgagcaggac gacgtctggc
    31861 gcctgttttt cgaccagccg cagcgcttca tccacacttt cacaggactc caccgcaaag
    31921 ttcggctggt cggtgagcac acgcgcgact ccctcacgga acagggcatg gtcgtctaca
    31981 attagcacgc gcagcggctt cggcagcgtt cggttcatgg cgcgcccttc cgctcctcac
    32041 tagcgggccg caactccacc acaaaacagc tcccggtggc ttgaggctcg tagcgcaggt
    32101 ctccgtcgaa cgaccgtgca atcgcccgcg acacatagag gccgacgccg ctgtcttcgg
    32161 cccccgcctg gaacggacgg aagagattcg cggtatccgt caccccgcag cctgtgtccc
    32221 gaaaccgcac cagaaccgta tccctgtccc ggacggcctg caccgtgaac tccttgctca
    32281 cactttctcg catcgcccgc tcgctgttac gggccaggtt gagaaacacc tggagcaggc
    32341 tgtggtggtt cgcgcgcacc agcggcaggc ctggttccgc ttcccactgg accaagacgc
    32401 tgaactcgcg aaacgacgat tcgatgatga tccgggcctc gtccagcact gtttccaggt
    32461 ccacccactc gggctgcctg tttgccgcca gccggaggcc ggaggtggct acctgctcca
    32521 tggccttcac cagggcgccg agggcctgaa aatccttgtt ccccaccagc gccggccggc
    32581 cctccagcgc cccgtacgcc gaggccgccg ccgaggccag gttgcggacc tcgtgcgaga
    32641 ccgctccgat cagcacccgg gaggtcgaca tcaacgattt aagcccggca tcttcccggt
    32701 cccgcaagtt ctcggaggcg tcccaaatta ccgccgccag ccgggcgccc ccgccgatcc
    32761 ggtacgtaga gaaccagact tgcgccagaa acacctcccc gtcgcggcgc tgcccccggc
    32821 actcgatcgt cgtccgcaag cttcggccgg cgtccccgtt ccggagcggg cgccccagaa
    32881 ccggcaaata ctggccgatg tcccttccct ggagtgcatc cccgtcgaga aacccgagga
    32941 gccggttcgc gctatggttg gccaggatca cggttccgga ggaatccagc gtgacgatgg
    33001 ccagtgggct ggtttcaatc aggacgcgga gttgctgctc tgcctcctcc cgcagcttca
    33061 ggtttcggac cgccagcctc cggctgtgac tcagctcgaa aaccagcagc ccggccccgg
    33121 aaaagcctcc cgccacgatg agcagccgtg ctgccattcc cggctctcct ataaagggtc
    33181 ccagggcctc ccgaagaacg gcgcaggcgg ccgatagcgt gacaatctgc cagcggcgaa
    33241 gagtcaccga agcgagcagg atcgggacca tgtagagcac gccaagtgac acgttgggct
    33301 ggattttcca gtcggcaagc gcgatacaca gggtcaaccc ggcagccagc gccagcactc
    33361 ctgtccggtg agcttccgaa tacgccagaa aaccctcgaa cgggctcgtt cgacgttcgc
    33421 cttgtggtgt ggcagcattc gaagacatgg cgcggtcggc ggtaaacagt atatctcccc
    33481 aacttgcggc gaaataggcc aaacctggac ccgtttccta gtactttgcg gatcgcaaag
    33541 gacgatcccg tactatacgt cccggaggtg ctcaaacctg aaagatgaca ttgccggtag
    33601 tccgtccggg ttcgacaatc gagtagcccc gcccgtggtg tgtggacggg gtgcgaagtt
    33661 tcacttgatt tggaggagtc agttccatgc gacgtgttct tcggtatggt ccggccgcgg
    33721 ttctcatctt gttggtcttt gttggagtcg tgcttcaggg ctcgctcccg gtagcttcca
    33781 tcaatgcctg ggtcgctggc ggcagcccgc tcgcgcagcg cacgggcgcc tgcgccgtca
    33841 cgcttgccga cggtaacgtg ctgattgccg gcggcgtcga tgccggcggc aacccgctcg
    33901 cgagcgcgga aatcgtcagc aagtttagcg gttcgttctc ggctgcggct tcgatgctgg
    33961 cggcgcgcag caggcatgcg tgcgccgcgc tgccggatgg gcgcgtcctc gtgatgggcg
    34021 gcgtcgtgtc cggcggcgtc accaatgaca ccgagatcta cgacccgtcg agcggcacat
    34081 gggccgctgg tccgccgatg tcgggtacgg ggcgggccgg ccacacggct tccgtgctgt
    34141 ccgacggcaa agtggtgatc gctggcggcg aaaacggcag tgcagtgctc gcgtccgtgg
    34201 agatctacga ccccgaggcc aacagcttcg ctccggcggg tgcgctcaac gagccacgca
    34261 aggaccacgg cgccgcagcg actctggacg gttgggtttg gatcgggggc ggccacaacg
    34321 gcacagaaga gctagcatcc gcggaggtct
//
LOCUS       DATIFQ010000478         3545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2078589, whole genome shotgun sequence.
ACCESSION   DATIFQ010000478 DATIFQ010000000
VERSION     DATIFQ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078589"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1970
                     /locus_tag="VLE22_24405"
     CDS             195..1970
                     /locus_tag="VLE22_24405"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSB17615.1"
                     /translation="MNARSFPRVGFVVLLALVWAVPAAPQVVGGVVGGPPAQQRPGIP
                     SPRDPREQRPQVGTASLTGRVVAADTGTPLRRAQVRVSGQDLRGARSAMTDGEGRYTV
                     GNLPAGRYSISFMKGGFANMSYGQKRPNQPGKSVDLLDGQKMENLDVSLIRGGVVTGR
                     IVDDFGEPIVDARVQVLQLRWFNGRRRMAGGGRGNQTNDRGEYRIWGLGPGEYYVSAT
                     STDRQMFSDPQLASLDATDSSGFAATYFPGTASVDEAQKVSVAAGQEVSGVDFALVTT
                     RVVRVSGTAMASDGRPMANAMVMMVSRGAMEMGGMMMSLGGRTDSDGSFVIPNVAPGE
                     YVLQARAEGGPRGMRGGGEGASTTVSVGTEDVKNVMLVATKGVRVSGRVIFEGSTPTA
                     EVKDRSRVFLPPIEREGFMGVGESGDAQVTPEGTFEVRGVSGRRTFSVSAGPPWMVKS
                     ARIGGTDVLDSGYEFGKEDVTNVEIVLTSRAPALTGSVTGENNSPVSDYVVVAFSTNE
                     ESWERLLGGMGRGFSIGRPDQNGTYKVTGIRPGSYYVVALPEMPEELGNPDLFKALKE
                     RAKQVKLQEGETERLDLVLQQAPGT"
     gene            2072..2416
                     /locus_tag="VLE22_24410"
     CDS             2072..2416
                     /locus_tag="VLE22_24410"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSB17616.1"
                     /translation="MGATDGLYLISVAARMLDMHPQTLRKYERLGLVRPSRTLGSMRV
                     YSHDEVERLRMIKRLVDEMGVNLAGVQRLLDVAEVAQRIEPLLRDQSLPRQELRKRLA
                     REFDRLCDIVGL"
     gene            2422..3420
                     /locus_tag="VLE22_24415"
     CDS             2422..3420
                     /locus_tag="VLE22_24415"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HSB17617.1"
                     /translation="MEFKDYYKTLGVSKTASEKELKAAYRKLARKHHPDVNPGDKGAE
                     AKFKELNEAYEVLGDPEKRKKYDELGANWRQYEQAQQAGWGPGGPSAGSPFGGRYSGG
                     AWNVNVGGPGGYRTMTPDEMEEMFGGSGGESPFSDFFKTFFGGDFAREERARTSPGRD
                     LEHELELSLEEAFSGTMRRLTIQQDGQARTIEVRIPAGVKTGSRVRAAGGGEAGSHGG
                     AAGDLYLRVRLRPHPVFEVRDHDLYAKVPVPLTTAVLGGEAQVPTLTGPVRLKIPSLT
                     QEGQTFRLKGHGMPTVGKPKERGDLYATAYVVLPKKLSDQERKLFEQLKEMERKGE"
BASE COUNT          699 a         1026 c         1227 g          593 t
ORIGIN      
        1 ggcaaacgac gacccgatct tccttgacat gatggcggac gtggtgcgca gcacttacga
       61 gcattaccgg gatgcgaggc cgctcacgat cgccatgccg tagaaccggc cgaaccaacc
      121 gtcagaacca accgtcagaa cttagagcgc ggacgtatac tgcgcccaga cccgcttttg
      181 ccggagaaat cgagatgaac gccaggagtt ttccgagggt cggttttgtg gtcctgcttg
      241 cccttgtgtg ggccgtacca gccgcgccac aagtcgttgg cggcgtggtg ggcggccccc
      301 cggcgcagca gcgccccggg attccgtccc cacgcgatcc gcgagagcag cggccgcaag
      361 tgggcactgc aagcctcact ggccgcgtcg tcgcggccga taccggcacc ccgctgagac
      421 gtgcgcaggt ccgcgtcagc ggacaggacc tgcgcggcgc acgcagtgcg atgaccgacg
      481 gtgaaggccg atacaccgtc gggaacctcc ccgccggtcg atactcgatt tcctttatga
      541 aggggggctt cgccaacatg tcatacgggc agaagcgccc gaaccagccc ggaaagtcgg
      601 tggaccttct ggacggccag aagatggaga acctggatgt ctctctcatc cgggggggcg
      661 tggtgaccgg ccggatcgtg gacgacttcg gcgagcctat cgtggatgcg cgggtccagg
      721 tccttcaact gcgatggttc aacggccggc gccgaatggc gggagggggg cgcggaaacc
      781 agacgaacga ccgcggcgag taccgcattt ggggtcttgg cccgggcgag tactacgtca
      841 gcgcgacgag caccgatcgc cagatgttct ccgacccgca gctcgcctcg ctcgatgcca
      901 ccgattcctc cggctttgcg gccacctact ttcccggaac ggcgagtgtc gatgaagcgc
      961 agaaagtgag cgttgctgca ggccaggagg tcagcggcgt ggacttcgcg ctggtgacca
     1021 cgcgcgtcgt ccgcgtctcg ggcacagcca tggcgagcga cggacgcccg atggcgaatg
     1081 cgatggtgat gatggtgtca cgaggggcga tggagatggg cggcatgatg atgtcgctcg
     1141 gaggacgcac cgacagcgat ggctccttcg tgattccgaa tgtggcgccc ggtgagtacg
     1201 tgctgcaggc tcgagccgaa ggcggcccac gcggcatgag aggcggaggt gaaggcgcca
     1261 gcaccacggt ttcggtcggt accgaagacg tgaagaatgt gatgctggta gcgaccaaag
     1321 gggtgcgcgt cagcggtcgc gtgatctttg agggttcgac accgacggca gaggtgaagg
     1381 accgctcgcg cgtatttctg ccgccgatag agcgcgaagg attcatgggg gttggagagt
     1441 ccggtgatgc gcaagtgacg cccgagggta ccttcgaggt gcgcggcgtc tccggtcgcc
     1501 gcaccttcag cgtctcggcc ggtcccccgt ggatggtcaa gtctgccagg atcggcggca
     1561 ccgacgttct cgacagcggc tacgaattcg gcaaagaaga cgtgaccaat gttgaaatcg
     1621 tgctgaccag ccgtgcgccg gcgctcaccg gatctgtcac cggcgagaac aattcgcccg
     1681 tatccgacta tgtcgtcgtc gctttctcta caaacgaaga atcctgggaa cgcctgctcg
     1741 ggggaatggg gcggggcttc agcatcggtc gtcccgacca gaatggtacc tacaaagtga
     1801 cgggcattcg acccggctcg tattatgtcg tcgctctccc agagatgccc gaggaactcg
     1861 ggaaccctga cctcttcaag gcgctgaaag agcgggcgaa gcaggtgaag ctgcaggaag
     1921 gcgaaacgga acgcctcgat ctggtgttgc agcaggcgcc gggaacgtaa cgggacagtc
     1981 ctccaattct ccgtttttgt ggcaatttgt aggtggtcct gctcaccttg acacgcgccg
     2041 tgtcaactcc tatattcaaa gcaacgcaag tatgggagcg acggacggtc tttacctgat
     2101 cagcgtggcg gcgcggatgt tggacatgca tccgcagacg ctgcgcaagt acgaacggct
     2161 cggcctcgtg cgcccttcgc gcacgcttgg cagcatgcgg gtgtactcgc acgacgaggt
     2221 ggaacgcctc cggatgatca aacggctggt ggacgagatg ggagtgaacc tggccggagt
     2281 ccagcggttg ctggacgtgg cggaggtcgc gcagcggatt gagcccttac tccgggacca
     2341 gtcgctcccc cgccaggaat tgcggaagcg cctcgcgcgg gagttcgatc ggctctgcga
     2401 catcgtggga ctgtaaagcg cgtggaattc aaggattact acaagacact cggcgtttcg
     2461 aagacggctt ccgaaaaaga attaaaagcc gcgtaccgga agctcgcgcg gaaacaccat
     2521 ccggacgtca atcccggtga caagggcgcg gaggccaagt tcaaggaact gaacgaggcg
     2581 tacgaggtgc tcggcgatcc ggagaaacgc aagaagtacg acgaactggg cgcgaactgg
     2641 cgacagtacg agcaggcgca acaggccggc tggggaccgg gcggcccgtc ggccggcagt
     2701 cctttcggcg gccgctacag cggcggggcg tggaacgtga acgtcggtgg tcccggcggc
     2761 tatcggacga tgacgccgga cgagatggaa gagatgttcg gcggcagcgg aggagagtcg
     2821 ccgttctcgg acttcttcaa gacgttcttc ggcggagact ttgcgcgcga ggagcgcgcc
     2881 cgtacctctc cagggcgaga tctcgagcac gaactcgaac tctcgctcga agaggcgttc
     2941 tccggcacga tgcggcggct cacgatccag caggacggcc aggcacgcac gatcgaggtt
     3001 cggattccag ccggcgtgaa gaccggatcg cgcgtgcggg cggcgggtgg cggcgaagca
     3061 ggcagtcatg gcggtgcggc cggagactta tatctgaggg tacggctacg ccctcatcca
     3121 gtgttcgaag tacgcgacca cgacctttac gcgaaggtcc cggtgccgct caccacggcg
     3181 gtgctcggcg gcgaggcgca ggtgcccacg ctgaccggcc cggtccgtct gaagattccc
     3241 tcgctgacgc aggaaggtca gacgtttcgg ctcaaggggc acggcatgcc caccgtcggg
     3301 aagccgaagg agcgcggtga tctctacgcc acggcgtacg tggtgctgcc gaagaaactc
     3361 agcgaccagg agcggaagct gttcgagcag ctgaaagaga tggagaggaa gggggagtga
     3421 aaggttcgaa aggttcgaag ggttcgaagg ttcgacgggt tcgtggttcg tggttcgata
     3481 ggttcgaggt tcgaaccgtg aacctgtcga accgtgaacc cgtcgaacct atcgaaccgt
     3541 gaacc
//
LOCUS       DATIFQ010000479         4397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_354189, whole genome shotgun sequence.
ACCESSION   DATIFQ010000479 DATIFQ010000000
VERSION     DATIFQ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354189"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VLE22_24420"
     CDS             <1..766
                     /locus_tag="VLE22_24420"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSB17618.1"
                     /translation="FEDFKAEMRNDFLKRRVIGQEVGSRISIPRAEARDYYDKHKEEF
                     VREERVFLSEILLSTQGKSGSDPAVLEKKAKELVTRARNGERFGELARDNSDAESSST
                     QGHLDAFKRTDLNKDIAQIVFSNDRGYVTDPIKVANGFLILRVDERHQAGQAAFEEVE
                     NEIMEKLYMPRFEPAIRTYLTKLRGEAFLEIREGYVDTGAAPGKDTGWRDPAQLRPQT
                     VTREEIATSIRRKRLLWLMPLPGTKTSSRESSSVQQ"
     gene            782..1885
                     /locus_tag="VLE22_24425"
     CDS             782..1885
                     /locus_tag="VLE22_24425"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSB17619.1"
                     /translation="MKSPYVRDLQPNQIVTATFLVHAKEIRQKKTGDPYLSLLLGDRT
                     GDVDGKMWDNVGEVMDTFDRDDFVRIKALLSIYQNRPQLTIHKLQRLDPAEVDPGDFF
                     PVSTRDPEEMWAEVRQIVAGFTNPHLKALVEALIDDPEIATLYRRAPAAKMIHHAYLG
                     GLIEHVLSLCTLCRFMAQHYSNIDLDLLLTGALLHDIGKLYELSYDRSFGYTTEGQLL
                     GHIAIGVRLIEQKLGQLPDFPPRLRALVEHMVLSHHGQLEFGSPKVPLFPEAMLLHYL
                     DDLDSKMECMRALVEHDRQVEGDWTSYSHSLERTVLKKLRYLEGRPELDPPEPALVPP
                     TPAATKPPARPNRPERGPSLFGEKLIDALGKEQ"
     gene            1886..2293
                     /locus_tag="VLE22_24430"
     CDS             1886..2293
                     /locus_tag="VLE22_24430"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HSB17620.1"
                     /translation="MRRPAPPREIPPPLELECLKEIWRLGEGSVKDVRDSLAPRRHLA
                     YTTVMTLLERLVRKGAASRRKTGRSFLYAPILSRETLRRLAVRELVESLFEGSEYELA
                     AYLRNGELLEPDHSDGLESEPIAAGEKLDTALL"
     gene            complement(2294..2899)
                     /locus_tag="VLE22_24435"
     CDS             complement(2294..2899)
                     /locus_tag="VLE22_24435"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSB17621.1"
                     /translation="MTRTIIFDLGQVIVPFDFQRGYAAIAAECVHPPEEIRRRLRETG
                     LVLPFEEGKVAPGEFVRRVTEALDLPVSYERFCEMWSSIFLPETLIPESMVASLHERY
                     RVLLLSNTNVIHFEMIRDKYPIMRHFDHCVLSYEVGAMKPAPEIYREAVIRAECNPEE
                     IFFTDDIAMNVEAARTHGIDAIQFQSLPQLEDDMRSRGIVW"
     gene            2960..3057
                     /gene="ffs"
                     /locus_tag="VLE22_24440"
     ncRNA           2960..3057
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VLE22_24440"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3121..>4397
                     /gene="dnaX"
                     /locus_tag="VLE22_24445"
     CDS             3121..>4397
                     /gene="dnaX"
                     /locus_tag="VLE22_24445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HSB17622.1"
                     /translation="MYQVIARKYRPQTFAELVSQEHVKTTLENAIRQNRIAHGYIFSG
                     QRGTGKTTVARILARCLNCVDGPSATPCGTCASCVEIANGNAPDVIEIDAASNRGINE
                     MRELRENVRYRPARDRHKVFIIDEAHQITSEAFNALLKTLEEPPEWVVFILCTTEAHK
                     IPSTIASRCQHFSFRSVDFEDVVSRMEYICREEGIQADAEVLAVLAQAGDGSVRDSLS
                     ALDQAIACCGTELKADEVRALLGAFSLDSLHEVTEALGQSDSAKMLAVVDELERNGHN
                     LQHFCRELARYFRNLVVAKIAGSSTRLIAASPAERERLAEIAASFSQEDLTRYLQIAL
                     DLYGDLQRSLQPRLHMEIGLLRMVQAGRLIAIEEALSMVGGGGSQSPPSPPPPPARRS
                     EPPRAGAVSRTTQPPTTPATAKPTSPTLGDSGW"
BASE COUNT          920 a         1365 c         1345 g          767 t
ORIGIN      
        1 gtttgaggac ttcaaggcgg aaatgcgaaa cgacttcctg aagaggcgcg taatcgggca
       61 ggaagtcggg tcaaggatca gcattccccg ggctgaagct cgcgactatt acgacaagca
      121 caaggaagag tttgtgcgcg aagagcgggt ttttctcagc gagatcttgc tctcgacgca
      181 gggcaagagc ggaagcgatc ccgcggtgct ggaaaagaag gccaaggaac tcgttacccg
      241 ggcgcgcaac ggggagcgct tcggcgaact tgctcgcgac aactccgacg cagaatcgtc
      301 gagcactcag gggcacctgg acgccttcaa gcgcacggac ctgaacaaag atatcgccca
      361 aatcgtcttc agcaacgatc ggggctatgt gaccgacccg attaaagtcg cgaacgggtt
      421 tttgatcctg cgggtggacg agcggcatca ggccggtcag gctgccttcg aagaagtaga
      481 gaacgagatc atggaaaagc tttacatgcc ccgtttcgag cccgccatcc ggacctactt
      541 gaccaagctt cgaggggagg cgtttctgga gatccgggaa ggctacgtcg acaccggtgc
      601 ggcgcccggc aaggatacgg gatggaggga tccggcacag ctcaggccgc aaacggtcac
      661 gcgggaagag atcgcgacca gcattcgccg gaaacgtctg ctctggctca tgccgctgcc
      721 cggcaccaag acgtcttcgc gagaaagcag tagcgtgcaa cagtaggcgc cggcactccc
      781 gatgaaatct ccttacgtcc gcgatcttca accgaaccag atcgttaccg cgacgtttct
      841 ggttcacgca aaagagattc gccagaagaa aacgggcgat ccctatctgt cactgttgct
      901 cggcgaccgg accggcgacg tcgacggcaa gatgtgggac aacgtgggcg aggtgatgga
      961 taccttcgac cgcgacgact tcgtgcggat caaggctctg ctcagcatct atcagaaccg
     1021 gccacagctc acgatccaca aactgcagcg gctcgatccg gccgaggtcg atccgggcga
     1081 ttttttcccg gtctccaccc gcgacccgga ggagatgtgg gcggaggtcc ggcaaatcgt
     1141 cgccggcttc accaatcccc atctcaaagc gctggtggaa gcgctgatcg atgaccccga
     1201 gatcgccaca ctctaccgcc gagccccggc ggcgaagatg atccaccatg cgtatctggg
     1261 aggcttgatc gagcatgtgc tgtcgctgtg cacactctgc cgcttcatgg cgcaacacta
     1321 cagcaacatc gacctcgacc ttctgctcac cggggcgctg ctgcacgaca tcggcaagct
     1381 gtacgaactg agctacgacc gaagcttcgg ctataccacg gaagggcagt tgctagggca
     1441 catcgccatc ggcgtgcgtc tgatcgagca aaaactgggc cagttacccg acttcccgcc
     1501 gcggcttcgg gcgctggtgg agcacatggt gttgagccac cacggacaac tggagttcgg
     1561 ctcgccgaaa gtgcctctct ttcccgaggc gatgcttcta cactacctgg acgatctgga
     1621 ctccaagatg gagtgcatgc gcgccctcgt cgagcacgac cggcaggtgg agggtgactg
     1681 gacgagttac agccactcgc tggaacggac ggtcctcaag aagctgaggt atctggaggg
     1741 acgaccggaa ttggatcctc cggagccagc gctggtaccg ccaacgccag cggccacgaa
     1801 gccgcccgcg cgcccgaatc gaccagagcg gggaccgagc ctcttcggag aaaagctgat
     1861 agacgctctc ggcaaggagc agtaaatgcg caggccggcg ccgccccgtg aaatcccgcc
     1921 tcctctcgaa ctggaatgcc tgaaggagat ctggcggttg ggcgagggca gcgtcaagga
     1981 tgtccgtgac tctcttgctc cgcggcggca tctcgcctac acgaccgtga tgacgttact
     2041 cgaacgtctg gtacgcaagg gcgccgcgtc ccggcggaaa accggcaggt cgttcctgta
     2101 tgctccgatc ctctccaggg aaaccctgcg ccggttggcc gtccgggaac tcgtcgaatc
     2161 gctgttcgaa gggtccgaat acgaactcgc ggcctatctc cggaacggcg agttacttga
     2221 gccggatcat tcagacgggt tggaatccga acctatcgcc gccggcgaga aactcgacac
     2281 cgctcttctc tgatcaccag acgatcccgc gggagcgcat gtcatcttcc agttgaggca
     2341 gggactggaa ctggattgca tcgattccat gtgttcgcgc cgcctccacg ttcatggcga
     2401 tatcgtccgt gaagaagatc tcctcaggat tgcattcggc tcggatgacc gcttcacgat
     2461 agatctccgg cgccggcttc atggcgccca cttcgtagga cagcacacag tggtcgaagt
     2521 ggcgcatgat cggatacttg tcgcgaatca tctcgaaatg gatgacgttg gtgttggata
     2581 gcagcagtac acggtaacgc tcatgcaggg aggcgaccat cgactctgga atcagagtct
     2641 ccggaagaaa aatactgctc cacatctcgc agaaccgctc gtagctgacc ggaaggtcga
     2701 gggcctcggt gacccggcga acgaactcgc ccggggcgac tttgccttcc tcgaatggca
     2761 gtacaaggcc cgtctcccgg aggcgccggc ggatctcctc cggtggatgc acgcactcgg
     2821 cggcgatggc cgcgtatccg cgctggaagt caaacggcac gatgacctgc cccaggtcga
     2881 agatgatggt ccgggtcaca gttcgagtgt aacgaatcgg tcgccggagc ccggcgtggt
     2941 agcatgagaa gttgtcaggc tccgggcttc gtgcaacggg ccgctgcaaa ccccgccagg
     3001 tccggaagga agcaacggta gtggcccagc ccgtgtgccg cggatcaccc ggggcctggc
     3061 attctgtcgt cgccgcctga agcagaaacc ggacagccgg aaaccgtaga gccgatctcc
     3121 atgtaccagg tcattgcacg aaaatatcgt cctcagacct tcgcggagct ggtgagccag
     3181 gagcacgtca agactaccct ggaaaatgcg atccggcaga accggatcgc tcacgggtac
     3241 atcttttccg gccagcgggg caccggcaaa acgaccgtgg cccgcattct cgcacgctgc
     3301 ctgaactgtg tcgacggacc ctccgccacc ccgtgcggga cgtgcgcaag ctgcgtggag
     3361 atcgccaacg gaaacgcccc ggacgttatt gagatcgacg ccgcctctaa ccgcggtatc
     3421 aacgagatgc gggaattgcg ggagaacgtc cgctaccgtc ccgcgcgcga tcggcacaag
     3481 gtgtttatca tcgacgaggc gcaccagatc actagcgaag cgttcaacgc cttgttgaag
     3541 acgctcgagg agccgccgga gtgggtggtg tttatcctct gcaccaccga ggcacacaag
     3601 atcccgtcaa caatcgcgtc acgctgccag cacttcagct tccgttcagt ggacttcgag
     3661 gacgtcgttt cccgcatgga atacatctgc cgggaggaag ggatccaggc cgacgcagag
     3721 gtgctcgccg tgctcgccca ggccggagac ggcagtgtgc gggactccct ctctgcgctt
     3781 gaccaggcga tcgcctgctg cggaaccgaa ctcaaggcgg atgaggttcg agcgctgcta
     3841 ggcgcgttct cgctcgactc gctccacgag gtaaccgagg cgcttggcca gtccgactcg
     3901 gccaaaatgc tggcggtggt cgacgaactc gagcgcaacg gacacaacct tcagcacttc
     3961 tgccgagaac tggcccgcta ctttcgaaac ctggtggtcg caaagatcgc cggttcctcg
     4021 acgcggctga tcgccgcttc gcccgccgag cgagagcggc tcgcagagat cgccgcttcg
     4081 ttctcgcagg aggatctgac acgttacctt cagatcgccc tcgatctgta cggcgatctc
     4141 cagcgttctc tccaaccacg gcttcacatg gagatcggac tgctccgaat ggtgcaggcc
     4201 ggccggctga tagcaatcga agaggccctg tcgatggtcg gtggcggagg gtctcagtct
     4261 ccgccgtctc cgccgcctcc accggcccgc cgctccgagc cgccgcgtgc cggagcggta
     4321 tcgcgtacaa cccagccgcc aaccacgccc gcaacagcga agcccacctc tccaacacta
     4381 ggcgacagcg gatggaa
//
LOCUS       DATIFQ010000480         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2079812, whole genome shotgun sequence.
ACCESSION   DATIFQ010000480 DATIFQ010000000
VERSION     DATIFQ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079812"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..2114
                     /locus_tag="VLE22_24450"
     CDS             153..2114
                     /locus_tag="VLE22_24450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17623.1"
                     /translation="MARSTNASIGFLDHETTALRVNVSGTTHRLATPRKNSGALFMVI
                     ALGLAAGVPTAQGQEISFLREFSISDWGAAHRVVVDTSGIYVGGGGSGGIFVRKYSAS
                     GNELWTRQIPATNGGWTTGMAVDANGVYVVSSTRGKSSVHKYDTSGNELWARPISPSQ
                     SADATGAAADATGLYVAGGLGQLAFLRKYDSSGTELWTRQSSSGIYYAGVAADSTGVY
                     LVGNIVDGRITHGLVRKYTAGGEEVWTRQFLGANWARGVAVDTTGVYVVASSSQLFLR
                     KYDSSGNESWTREFDKSSVTPVSSDDYVDVTGVAADPSGVYLVGATSFALAGQCRSGF
                     QDAFVRKYDADGVSVWTRQFSASSDYGSGSGVAVDATGVYVAGQSTGPPGSKALLAKL
                     EKTQPAINESVPRIFPGCVVNAASDIGGRVSPGEIVTILGRGIGPLETAPLLLTEQHR
                     LATTLAGTRILFNGIPAPLISVAADRATAIIPYVVAERSTVDVQVDYQGVLSNVVTMP
                     VAPVRPGIFTVGSPDGRHAAALNEDGSINSSSTPATLGSVIVLFATGEGLTEPVVEDG
                     LVLGSMLPKPRLPVSVVFSSSCSGFYCYYYYYGRGPESEEALYAGGAPGLVAGVLQVN
                     VRIPRNASDEVKLCIDDQCTEFTRVFIKH"
     gene            2467..>3264
                     /locus_tag="VLE22_24455"
     CDS             2467..>3264
                     /locus_tag="VLE22_24455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17624.1"
                     /translation="MLRSTSAAIVFPDHGTTALPAKVSGTVSRLTTPKKGPGVLFMLV
                     ALGVSASVRTAQGQEISFLREFRIADWGEGQKVAVDATGIYVAGYGSNGSFVRKFDPS
                     GNELWTRQVQATEWGFAGGVAADAKSVYVAWSSGRRAFLRKYDPNGNELWVGESTGGV
                     SYASLAADSTGVYVVGGKGQLAFLRKYDASGNELWTHQSATGTYFAGVAADSTGVYLI
                     GNTAGGRTNQGFVRKYTAGGAELWTNQFSGVNWATSVTTDATGVYVAG"
BASE COUNT          645 a          921 c         1068 g          630 t
ORIGIN      
        1 tagacccccc taccttctcg gataggcgcg cattgccgcc atcgcgttaa gcggaagagc
       61 gtctccctcc agtttcagta agttgcgcgg gccgtccgtg ctccggcccg ctggcaagct
      121 ggttgccaca gctgagtcga aggaggctca acatggcgcg aagtacgaat gcttccatag
      181 ggtttctcga tcacgaaacc accgcccttc gggtcaacgt atcggggaca acgcaccggc
      241 tagcgacgcc aaggaagaac tctggcgccc tgttcatggt gattgctctc ggcctagcgg
      301 ccggcgtccc cactgctcaa ggacaagaga tttcgttcct gcgcgagttc agcatttcgg
      361 attggggcgc ggcccataga gtggtcgtgg acaccagcgg catctacgtg gggggcgggg
      421 ggagtggcgg catctttgtg cgaaagtaca gcgccagtgg aaacgagttg tggacacgtc
      481 agatcccggc cacgaacggg ggttggacga ccggaatggc ggtagacgcg aacggcgtct
      541 acgtggtttc gagcacccgc gggaaatctt cagtgcacaa gtacgatacc agcgggaacg
      601 agctttgggc ccgtccaatt tccccgagcc aatctgcgga cgcgaccggt gcggcggcag
      661 atgcgacggg cctctacgta gcgggcggat tggggcaact ggcgttcctg cgcaagtacg
      721 actcgagcgg aaccgaactt tggacccgcc agtcttcaag cggcatttac tacgcgggcg
      781 tggcggcgga ttcaacaggc gtctacttag ttggaaacat tgtcgacggc cggatcaccc
      841 acggcttggt gcgcaagtac acggccggag gtgaagaggt ttggacccgc cagttcttgg
      901 gcgccaactg ggcgagaggc gtggcggtgg atacgacggg tgtctacgta gtggcatcga
      961 gttcgcaact gttcctgcgg aaatacgact cgagcggaaa tgagagttgg acccgcgagt
     1021 tcgacaaatc cagcgttaca ccggtcagtt ctgacgacta cgttgacgtg acaggcgtgg
     1081 cagcggaccc cagcggcgtc tatctcgtgg gcgctacaag ctttgcccta gccggccaat
     1141 gcagatccgg cttccaagac gcctttgtgc gcaagtatga cgccgatggt gtatctgttt
     1201 ggactcgcca gttctcagcc tcgtcggatt atggctccgg gagcggggtg gccgtagatg
     1261 ctacgggcgt ctacgtggcg ggccagtcga caggtccgcc tggctcaaag gctctgctgg
     1321 ccaagctcga gaagacgcag cctgcaatca atgaatcagt gccccggatc ttcccagggt
     1381 gcgttgtcaa cgcagccagc gacatcggtg gacgagtttc ccccggcgag atcgtgacga
     1441 ttctcggccg tgggatcgga ccgctggaga cggctccgct cctcctgacg gagcagcacc
     1501 ggctcgctac cacgctggcc ggcacccgca tcctcttcaa cggcatccct gcgccgctca
     1561 tctcggtcgc tgccgaccgg gcgaccgcca tcattccgta cgtggtggct gaaagatcga
     1621 cggtggacgt tcaggtcgac taccagggcg tgctttcgaa cgtggtgacg atgccggtgg
     1681 cacctgtgcg gcctggcatt ttcaccgtcg gtagccccga cggcagacac gccgcagcct
     1741 tgaacgaaga cgggagcatc aactcgtctt cgacgccggc aacactaggt tccgtcatcg
     1801 tcttatttgc gaccggagaa ggcctgaccg agccggttgt ggaggatggc ctggttcttg
     1861 gttccatgct gcctaagccg aggttgcccg tcagcgtagt gttttcttct tcgtgttccg
     1921 gcttctactg ctactactac tactacggaa gaggaccgga atccgaggaa gccctatacg
     1981 ccggaggggc gcctggattg gtcgcgggcg tgctccaggt gaatgtacga atacctcgga
     2041 atgcatccga tgaggtgaag ctctgtatcg atgatcagtg cacggagttc actcgtgtgt
     2101 tcatcaagca ctgatcagtt tcagtaagtt gcacaggctg accgtgctcc gaccactggc
     2161 acgcctgctg cctgtaacac ggctgcgagt gagaggaatg agcaacggca gaaggcattt
     2221 ggagttgaca tcggccatgc tcgatatgat tggccagcac cgaaggagat tgctttttct
     2281 gagactgact taggtgaatc aggagtatac ttatcaccaa gggaggggcg acgctgggta
     2341 gcggtccgcc gtacgtccgg caaaatcgaa cagctcaatt tggggtgcgc ggcaccgacg
     2401 gcgaggctga catagaggcg aaaacactca gagcggcccg catggccggg tcgaaggagg
     2461 ttcgggatgc tgcgaagtac gagtgcggcc atcgtattcc ccgatcacgg aaccaccgca
     2521 cttccggcaa aagtatcggg gacagtgagc cggctaacga cgccaaagaa aggccccggc
     2581 gtccttttca tgctggttgc tctcggcgtg tcggccagcg tccgcactgc ccaaggacaa
     2641 gagatttcgt tcctgcgcga gttcaggatt gcagattggg gcgagggcca gaaagtggct
     2701 gtcgatgcca ccggcattta cgtggcgggc tacgggagta acggcagctt tgtgcgcaag
     2761 ttcgacccga gcggaaacga gttgtggacc cgtcaggtcc aggccaccga atggggtttc
     2821 gcgggtggcg tggcggcaga cgcgaagagc gtctacgtgg cctggagttc cggccggcga
     2881 gcgttcttgc ggaaatacga tccgaacgga aacgaactct gggtcggcga gtctacaggc
     2941 ggcgtttcct acgccagcct ggcggcggat tcaacgggcg tctacgtggt gggcggaaag
     3001 ggtcagctag cgttcctgcg taagtacgac gcgagcggaa acgagctttg gacacaccag
     3061 tctgccaccg gtacttattt cgctggcgtg gcggcggatt caacaggcgt ctacttgatc
     3121 ggaaacacag ccggcggccg aaccaaccaa ggcttcgtgc gcaagtatac agccggcggc
     3181 gcagagctgt ggacgaacca gttctcgggc gtcaactggg cgactagtgt gacaacggat
     3241 gcgacgggcg tctacgtcgc cggc
//
LOCUS       DATIFQ010000481         7671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_737836, whole genome shotgun sequence.
ACCESSION   DATIFQ010000481 DATIFQ010000000
VERSION     DATIFQ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737836"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VLE22_24460"
     CDS             <1..1054
                     /locus_tag="VLE22_24460"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSB17625.1"
                     /translation="DDFVLLSDQPFSMPAVGEKNGDTSGLRYSAAHLSRRADVSPVFS
                     HPPNLQKGSGPRTSLERRWWLWGVAKEMARQRAGLFHGTHFSVPWLPLRPSVATVLDL
                     SPWCYPRWQRGAGRVQRLTPYQLRLGLASMVVTPTEAVRREAIEHFRLSPQRVVAVPL
                     AASPHFRPCPMGQSAVPYFLFVGMLEPRKNVETLVEAWREVRRTCAVDLVLAGRRRED
                     GPEIAAEPGIRVLGEVADSKLPGLYSGALACLYPSRYEGFGLPVLEAMQCGAAVIASR
                     DPAVTEVAGGAAVQVDADHRRGWIEAMVAAATQPDWREALRSRSLARAKAFSWQETAR
                     KTREVYAEAIHRFTTG"
     gene            1020..2270
                     /locus_tag="VLE22_24465"
     CDS             1020..2270
                     /locus_tag="VLE22_24465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB17626.1"
                     /translation="MRKPSTASLPVDAPDRLRNRDRQGAVAALLLAPEAPYPPVGGGA
                     LRSASLVEYIGRRYALDLIVFREPEAPDPAAAIPPGLVREIHVIELPRHSKRRMARAL
                     RNTSRLLRAIPPLNDRFGGFDEEIRRFVDGRRYQLAVIEHSWCAGYRDTLAPYCERLL
                     LDLHNIESALLAGCAEAESGAAAYALRRFRAACLDLEKLWFPRFDLLLAASEPDARRA
                     RAIAAGCSPCVYPNAIPSVPLPKAREDDAIVFSGNLGYQPNISAIRFFRKQVWPSLRE
                     RWPGIEWRVIGKNPHAVAGLLTNDARIRLLGPVEDAVAALAAAKVAVVPMLAGSGTRV
                     KILEAWAAGRAVVSTQLGAEGLPVRPGEHLLSADTPEEFTAGVSSLLESKQLRDRIGA
                     AGRDLYVTQFTWEAAWDRLRALGI"
     gene            2312..3469
                     /locus_tag="VLE22_24470"
     CDS             2312..3469
                     /locus_tag="VLE22_24470"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSB17627.1"
                     /translation="MRFSVDAHAIGRHLTGNEVYIRNLLTQFADLDKSAEFIAYLSVA
                     DADGWVPRRFLRRHVATNPFVRLGFDLSRRLGQDRPDLLHVQYNAPIGCPVPLVVSVH
                     DVSFLDHPEYFPPLRPQQLRYTVRRTIRRSARVLTGSEFSRSAIIRAYGLPDDKVLVV
                     PNAAEQRFRPISREAAAARVAERFRIPSPFVLTVGDLQPRKNHLGLISAFAEMLRAMP
                     SLPHHLVLAGKDSWHSSHVHRAARVSGVGDRIHFTGFVSDDDLLALYNACELFVFPSF
                     YEGFGLPVLEAMACGRAVACSNTSALPEVADAAAILFDPYSRDEATRAMLDLVLDGEL
                     RARMEKLGAHRASQFSWQKTAARTLEVYHDVADRQRVFQPEAERAAHALRS"
     gene            3466..4176
                     /locus_tag="VLE22_24475"
     CDS             3466..4176
                     /locus_tag="VLE22_24475"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HSB17628.1"
                     /translation="MTRILLAFAVLVLLLPSGRAEKPRHFSDETLSYSVNWPSGLSLG
                     EGRLVTRQVKGTSPAGDRWEFEFTLEAAIPGFQVIDHHRALASAVLCSIELEKEATHG
                     KRKTSERTIFEPQRGVASRQTLSGGGKSEMEISECPRDALTFLHHVRRELASGRVPPP
                     QTIYFGAPYTVHFEYGGRQNLRVGAAQVEADRLVASIRGKASDVTVELFFALDETRQP
                     VLVRVPLALGTFSLELVR"
     gene            4179..6155
                     /locus_tag="VLE22_24480"
     CDS             4179..6155
                     /locus_tag="VLE22_24480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB17629.1"
                     /translation="MRLAFFSPMPPSVSGIADYSEALLSSMRPLADITVFVDAAAPFD
                     PSKFDLALYHAGNNPHHAFVYETALRHPGVVVMHESSLHHLIADITIRHGDWDAYLRE
                     AEYNGGPEALARARRARALEIGPDYEGLPMTRRLLEASRAVIVHSRHMACEVRKAGFR
                     GPIARIPHGAWIPDVDRLAYRQRLGLDETTPLIGVFGHLKPYKRIAESLRAFRRLLRL
                     EPQAKMILGGEPHPDLPVASMIRGHDLSAAVRMLGFVPGEHLAGYISACDIVLNLRYP
                     TVGETSGTLLRALGLGRCVLVSEVGSFAELPDDICLKVPVGSGEEDLLFEYLNLLVSR
                     PEIAQAVGGRAKQWVERECNWDLVARRYAAFLECVHNGSEWTDEDAPAPTPEPEPEVA
                     GPPVDGGYILSWAANEESRAYVETHMTRLEKTLAITPLGGPDDRILEMGAYIQITPAL
                     RTKLGYGEVRACYYGQAGEVDEKRVATPEGEVFECKIELFDAEKDTFPYQNDHFSTVL
                     CCELIEHLANDPMHMMAEINRILKPGGHLVLTTPNIASIRGLAAILLGYHPGFFPAYI
                     RPSDGEETEARHNREYSPREVHLLLVDAGFEVVLLETGPFRDEPKPELLWVNHLLERY
                     KLSTELRGDGIYAVGRKTGAIKNRYPAWLYSGHA"
     gene            6155..7384
                     /locus_tag="VLE22_24485"
     CDS             6155..7384
                     /locus_tag="VLE22_24485"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17630.1"
                     /translation="MSATYHDLRVEPEAEGARLRVSFRIRNRSGEAWRGDDGFRFGYQ
                     IFDPETNAFIEEGQAVAPPRDIAPGETGTIEVGLQLPAVRGGYRVFVSPMVAGVRWNY
                     TRGWPFVLLDAAVEGGRAAVVRSLVTTVSRLRREARRRALAKVFVFPFMSMWRNRGMM
                     RSLVRRDILGRYRGSFGGAFWTLLNPLLLMLTYFFVFGIVLRARFSGDPSRSGFALYF
                     LAGMLPWLPFSEAAGRAPFVMLEHRNFVKKLVFPLETLPVNLALAGLVTQAFALVVFT
                     VLLVASRGYVPVSVAWLPVLLVPQVLLTVGLCWFLAALGVYVRDLGQVIGFALTLWFF
                     LTPICYPEGSLPPPALGILSKNPMFVLVRGYRAIFLESAAPSFAPMWKLWLLSAAVFF
                     AGHAWFYKLRKNFADVI"
     gene            7426..>7671
                     /locus_tag="VLE22_24490"
     CDS             7426..>7671
                     /locus_tag="VLE22_24490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17631.1"
                     /translation="MHRAWLLLLLTELAGFAQPTRPVYVIHRTPSPITIDAKLDEPAW
                     RNAPEAGEFYFNWWKEGEKERTAARLLWDADNLYVSYR"
BASE COUNT         1239 a         2512 c         2469 g         1451 t
ORIGIN      
        1 agacgacttc gtcctcctgt ccgatcagcc cttctccatg ccggcagtgg gcgaaaaaaa
       61 cggggacact tcgggcttgc gctacagtgc cgcacaccta tccaggcgtg cagacgtgtc
      121 cccggttttt tcgcaccccc ccaacctcca aaaaggcagc ggcccgcgaa cctccctcga
      181 acgacgctgg tggctctggg gcgtcgccaa ggaaatggca aggcagagag ccggactgtt
      241 tcacggcact cacttctcag tcccgtggct tccgctccgc ccgagcgtcg cgacggtgct
      301 cgatctgtct ccgtggtgct atccgaggtg gcagcgcggc gccgggaggg tgcagaggtt
      361 gacaccgtac cagctccgtc tcggactcgc cagtatggtg gtcactccca ctgaagccgt
      421 ccgtcgggaa gcaatcgagc acttccgtct ctcgccgcag cgcgtggtcg ccgtgccgct
      481 cgcggcatcc ccccatttcc ggccgtgccc gatgggccaa tccgcagttc catactttct
      541 gttcgtcggc atgctggagc cgcgcaagaa cgtcgagacg ctcgtcgaag cctggaggga
      601 ggtcaggcgg acatgcgccg ttgacctggt gctggcggga cggcgccggg aggacggccc
      661 ggagatcgca gccgagccgg ggattagggt gctcggcgaa gtagcagact cgaagctgcc
      721 cggtctttac tcgggggctc tcgcctgcct ctacccctcc cgctacgagg gtttcggact
      781 gccggtgctg gaggccatgc agtgtggcgc tgccgtgatt gcctcgcgcg acccagccgt
      841 cacggaggtg gcgggtggtg ccgccgtgca ggtcgacgcg gatcacaggc gcggatggat
      901 agaagcgatg gttgcggcag cgacacaacc tgattggcgc gaagcccttc ggtcgaggtc
      961 tctcgcgcgt gcgaaagcat tctcgtggca ggagacggcg cgaaaaacac gggaggtgta
     1021 tgcggaagcc atccaccgct tcactaccgg ttgacgcacc tgatcgcctc aggaaccgcg
     1081 accgtcaggg agcggtggcc gccctcctcc tcgcccccga agcgccctac ccgccggtgg
     1141 gcggtggcgc tcttcgctcg gcctcgctcg tcgaatacat cggccgccgt tacgctctcg
     1201 acctgatcgt gtttcgcgag ccggaagcgc ccgaccccgc ggccgctatc ccgcccggac
     1261 tcgtccgcga gattcatgtc atcgaactcc cccgccactc gaaacggcgg atggctcgtg
     1321 cgttgcgcaa caccagccgg ctgcttcggg caatcccgcc gctgaacgat cgattcggcg
     1381 ggttcgacga ggaaatccgg cggtttgtcg acggccgccg ttaccaactc gccgtcatcg
     1441 agcattcctg gtgcgcgggc taccgggata ctctcgcccc gtactgcgaa cggttgttac
     1501 tggatctgca caatatcgag tcggctctgc tggcaggctg tgccgaggcg gaaagcggag
     1561 cggccgcgta tgccctgcgc cggttccggg ccgcgtgcct tgacctcgag aagctgtggt
     1621 ttccgcgttt cgacctgttg ctggccgcgt cagagccgga tgcccgccgc gcccgggcaa
     1681 tcgcggccgg ttgctcgccg tgcgtctacc cgaacgcaat tccctcggtc ccgctgccaa
     1741 aggctcgcga agacgatgct atcgtcttct cgggcaatct cgggtaccag cccaacatct
     1801 cggcgattcg gttcttccgt aagcaggtct ggccttctct ccgggaacgc tggcccggca
     1861 tcgagtggcg cgtcatcggc aagaatccgc atgcggtggc tggcttgctg acgaatgacg
     1921 ctcgaatccg cctgttgggt ccggtggagg acgcagtcgc ggcgctcgct gcggcaaagg
     1981 tggcggtggt gccgatgctg gccggcagtg gcacccgcgt caagatcctc gaagcgtggg
     2041 cagcggggcg ggcggtggta tcgacgcagc ttggggcgga aggtctgccg gtacggcccg
     2101 gagaacatct cctgtcggcc gacactccgg aggagttcac tgccggtgtg tcgagcctgc
     2161 tcgagtcgaa acaactccgc gaccgcatcg gcgccgccgg tcgcgacctc tacgtaacac
     2221 aatttacatg ggaagcagca tgggaccgcc tccgcgccct agggatttaa ctagtgggaa
     2281 gggcctccac catctatact gaaagaatcg catgcgcttt tccgtagacg ctcacgccat
     2341 tggccgccac ctgacaggca acgaggtcta catcagaaat cttctcaccc agttcgccga
     2401 cttggacaag agcgccgagt tcatcgcgta cctgtctgtc gcggacgcgg acggctgggt
     2461 gccgcgccgt tttttgcggc gccacgtggc caccaatccg ttcgtccggc tcggattcga
     2521 cctctctcgc aggctcgggc aggaccggcc ggatctgcta cacgttcagt acaatgcgcc
     2581 gattggatgc cccgtgcccc tggtcgtgag cgtgcacgat gtcagtttcc tcgatcaccc
     2641 cgagtatttt ccgcctctgc gcccgcaaca attgcgctac accgtgcgcc gcacgattcg
     2701 ccgttcggcc agagtactca cgggaagcga gttctcccgc tcggcgatca tacgcgctta
     2761 cggccttccg gatgacaagg tcctggtcgt cccgaacgca gccgagcagc gcttccgccc
     2821 catttcgcgc gaagcggcgg ctgcgagggt agcggagcga ttccgaatcc cgtcgccgtt
     2881 tgtattgact gtaggcgacc tccagccgcg aaagaatcac ctcgggttga tttcggcatt
     2941 cgccgaaatg ctaagggcga tgcccagtct tccccatcac ctcgtactgg ctggaaagga
     3001 ctcgtggcat tcgagccatg tgcatcgggc ggcaagggtt tccggggtcg gggaccggat
     3061 tcacttcacc ggctttgtca gcgacgacga ccttctcgcg ctctataacg catgtgagct
     3121 tttcgttttc ccttccttct acgaaggctt cgggctgcca gtgctggagg cgatggcctg
     3181 cggtcgcgcg gtcgcctgct cgaacacgtc agctctcccg gaggtcgccg acgccgccgc
     3241 aatcctcttt gacccgtact ccagggacga agccacgcgg gctatgctcg atctggtcct
     3301 cgacggtgaa ctgcgggcgc ggatggagaa actcggtgcg caccgcgcca gccagttcag
     3361 ctggcagaag accgccgccc gtactctcga ggtctaccac gacgtcgcgg atcgccagcg
     3421 agtgttccag cccgaagccg agagggccgc tcacgctcta cggtcatgac aaggattctg
     3481 ctcgcgtttg cagtgctcgt actattgctt ccgtccggcc gggccgaaaa gccgcgccac
     3541 ttctccgatg aaacgctctc ctacagcgtg aactggccca gcggactcag cctcggcgag
     3601 ggccggttgg tgacccgtca ggtcaaggga acgtccccgg ccggcgatcg ctgggagttc
     3661 gagttcactc tcgaagccgc aatccccgga ttccaggtga tcgaccacca ccgcgcgctc
     3721 gcctcagccg tcctttgctc gattgagctc gagaaagaag ccacccacgg aaaacgaaaa
     3781 accagcgagc gtaccatctt cgagcctcag cgtggcgtcg ccagccggca gacgctcagc
     3841 ggcggaggca agtcggagat ggagatctcc gaatgcccgc gcgacgcgct cacgttcctg
     3901 catcatgtgc ggcgcgagct ggcgagcggc cgggtccctc ctccccagac gatctatttc
     3961 ggagcgcctt acaccgtcca cttcgagtat ggcgggcggc agaacctgcg cgtcggagca
     4021 gcgcaggtcg aagcggaccg cctggtcgcc tctatccgcg gcaaggcctc cgacgtcacc
     4081 gtcgagctgt tctttgcgct ggacgagacg cgccagccgg tcctggtgcg cgtcccgctg
     4141 gcgcttggaa cattcagcct ggaactcgtc cgctgaccat gcggttggct ttcttttcgc
     4201 ctatgccgcc ttccgtgagc ggtatcgccg actactcgga ggcgttgctc tcgtcgatgc
     4261 gcccgctcgc ggatatcacc gtcttcgtgg acgccgcggc tcccttcgac ccatccaagt
     4321 ttgacctcgc tctttaccac gcaggcaata atccccacca cgccttcgtc tatgagacgg
     4381 ccttgcggca ccccggcgtc gtggtgatgc acgaatccag cctgcaccat ttgatcgccg
     4441 acatcaccat ccgccacggc gactgggatg cgtacctccg ggaggccgaa tacaacggag
     4501 ggccggaggc gctggcccgg gcgcggcgcg cgcgcgccct cgagatcggc ccggattatg
     4561 agggtctccc gatgacgcgc cgactccttg aggcttcgcg cgcagtgatc gtccacagcc
     4621 ggcacatggc ctgcgaggtt cgcaaggccg gcttccgcgg acccattgcc cgcatcccgc
     4681 acggcgcttg gattccggac gtcgaccggc tggcctaccg gcaacgcctg gggctcgacg
     4741 agaccacgcc cctgatcggc gtcttcggcc atctgaagcc ttacaagcga atcgccgagt
     4801 ctctgcgggc atttcgccgg ctgctgcggc tggagcctca agcgaagatg atcctcggcg
     4861 gggagccgca cccggacctt cccgtcgcgt cgatgattcg cggccacgac ctgagcgccg
     4921 ctgtccggat gttgggcttc gtcccgggcg agcatctcgc aggatacatc tcggcctgcg
     4981 acatcgtgtt gaacctgcgc tacccgacgg tgggcgaaac gtcgggaacg cttctgcgcg
     5041 cgctcgggct ggggcgctgc gtcctggtgt cggaagtcgg ctcgttcgcc gaattgccgg
     5101 acgatatttg cctgaaggtg ccggtcgggt cgggcgaaga agaccttttg tttgagtatc
     5161 ttaacctgct cgtgtcaagg ccggaaatcg cccaggcggt cggtgggagg gcgaagcaat
     5221 gggtcgagcg ggagtgcaac tgggacctcg tggcgcggcg gtacgccgcg tttctcgagt
     5281 gcgtccacaa cggctccgag tggacggacg aggacgctcc ggccccgacg ccggagcctg
     5341 aacccgaggt ggcaggaccg cccgtggatg ggggctacat tctgagctgg gcggccaacg
     5401 aagagagccg ggcgtatgtc gagacgcata tgacgcggct cgaaaagacc ttggccatca
     5461 cgccccttgg cggccccgac gaccggatac tcgagatggg cgcctacatc cagatcaccc
     5521 cggcactccg cacgaagctc gggtacggag aggtgcgtgc ctgctactac ggccaagccg
     5581 gggaggtaga cgaaaagcgg gtggcgaccc ccgaaggcga ggttttcgag tgcaaaatcg
     5641 agctttttga cgccgaaaag gacacttttc cgtaccaaaa tgatcacttt tcgaccgtct
     5701 tgtgctgcga attgatcgaa catctggcga acgatccgat gcacatgatg gccgagatca
     5761 accggatcct caagccgggc ggccacctcg tcctgaccac ccccaacatc gcctcgatac
     5821 gcggactggc cgcaattctc ctcggctatc accccggctt cttcccggcc tacatccggc
     5881 cgtctgacgg cgaagaaacc gaggcccgcc ataaccgcga gtactcgccc cgcgaggtcc
     5941 acctgctgct cgtggacgcc ggattcgagg tggtcctgct cgagacgggt ccgttccgcg
     6001 acgaacccaa gccggaactg ctctgggtga accatctact ggagcgatac aagctctcca
     6061 ccgaactgcg cggcgacggc atctacgccg tgggccggaa gaccggggcg atcaagaacc
     6121 gctacccggc atggctgtat agtggtcacg cgtgatgagc gccacctatc acgacctgcg
     6181 cgtcgagccg gaagcggagg gcgcccggct gcgcgtctcg ttccgcatcc ggaaccggtc
     6241 cggggaagcg tggcgtggcg acgacggatt ccggttcggc taccagattt tcgaccccga
     6301 aacgaacgcg ttcatagagg aagggcaggc ggtggcgccg ccgcgcgaca tcgctccggg
     6361 agagaccgga accatcgagg tggggttgca gcttcccgcc gtccggggcg gttaccgcgt
     6421 gttcgtctcg ccgatggtgg ccggcgtccg gtggaattac acccgtggct ggccgttcgt
     6481 gctgctcgac gccgcagtcg agggtggccg agcggccgtg gtgcgaagtc tggtgactac
     6541 cgtgagccgg ctgcggcgtg aggcgcgccg ccgagccttg gcgaaggtct tcgtgttccc
     6601 gtttatgtcg atgtggcgga atcgcgggat gatgcgttcc ctggtgcgcc gcgacatcct
     6661 cggcaggtac cgcggttcgt tcggaggagc tttctggacg ctgctgaacc ccctgttgct
     6721 gatgctgacg tatttcttcg tgttcggcat tgtgctccgg gcgcggttca gcggcgaccc
     6781 gagccgctcg gggtttgccc tctacttcct tgccggaatg ctgccgtggc tgccgttcag
     6841 cgaagccgcg ggacgcgccc cgttcgtgat gctggagcac cggaacttcg ttaagaagct
     6901 cgtgtttccg ctggagacgc tgccggtgaa cctcgctctg gcggggctgg tgacgcaggc
     6961 gttcgcgctg gtggtgttca ccgtgctgct ggttgcgtcg cgaggctatg tgccagtgtc
     7021 ggtggcctgg ctgccggtgc tgctggtgcc ccaagtgctg ttgacggtgg gactttgctg
     7081 gtttctggcg gcgcttggcg tgtatgtccg cgacctcggc caggtgatcg ggttcgcgtt
     7141 gacgctctgg ttcttcctga cgccgatctg ttatccggag gggtcgttgc cgccgccggc
     7201 gctggggatt ctttcgaaga acccgatgtt cgtgctggtg cgcggctacc gggcgatctt
     7261 cctggaaagt gctgcgccct ccttcgcgcc aatgtggaag ctctggctgc tttccgcggc
     7321 cgttttcttc gccggtcacg cctggtttta caaacttcgg aaaaacttcg ccgacgtgat
     7381 ttgagacgct ccttgcggcg gccgccggcg cgataccctg gggtgatgca ccgtgcgtgg
     7441 ctgctgctcc tgctcaccga actggccggc ttcgcccagc cgactcgtcc cgtctacgta
     7501 atccaccgga cgccatctcc catcaccatc gacgcaaagc tggacgaacc cgcctggcgg
     7561 aacgctcccg aagcggggga gttctatttc aactggtgga aggaaggaga gaaggagcgg
     7621 accgccgccc gcctgctctg ggacgccgac aacctgtacg tcagctaccg a
//
LOCUS       DATIFQ010000482         2451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1693358, whole genome shotgun sequence.
ACCESSION   DATIFQ010000482 DATIFQ010000000
VERSION     DATIFQ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693358"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VLE22_24495"
     CDS             complement(<1..1295)
                     /locus_tag="VLE22_24495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17632.1"
                     /translation="MFQFRAEELSPGRGVRLVYQSGDTARVILLLIKIPVGNPRRYPF
                     AFQCDEPTQRRPYPLIRVVAAPGVSVVMKSFPLAWIQSRDTPAFEVIRVQDPEVIPPM
                     ATGVSTSAYCGREELNPPPEIRPLGMATVPPFASIPSMPGPANFIVRREPSVTYSDTY
                     IRHPYTGTLYRMRVPALTWNEDDNADAKARFAYFIDPDGALGSVRPLLKIVMTGPVEF
                     KWKQGPRRAADPPETVRALGLCDAFRVEDFSQVPVQGSDGSAWPLHPERPRRGFSTIP
                     RPTFPIDRIRDARTWYPAYAHRDATLREELATALIDLEVGLIPILGDAVDIGEFAYGL
                     LAGKDRWGRTLGVGDLAVMGVGAALPFVSGGALRAAGRLTPLFGPRRAGEFAELIEQL
                     RRVPWSDGDKATLTNLDQLVRQGVRLSNVQTQQFLALMKR"
     gene            complement(1554..1629)
                     /locus_tag="VLE22_24500"
     tRNA            complement(1554..1629)
                     /locus_tag="VLE22_24500"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1594..1596),aa:Phe,seq:gaa)
     gene            complement(1664..1933)
                     /locus_tag="VLE22_24505"
     CDS             complement(1664..1933)
                     /locus_tag="VLE22_24505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17633.1"
                     /translation="MAIPDDETGRPAAPVLQLSQPCRPTLDGLPIAALDGQDDLAPVT
                     QAGEDDEHDGLVLLEPGFDVDPVHPEVHGLQVSRPSRLDTPRSVW"
     gene            2010..2450
                     /locus_tag="VLE22_24510"
                     /pseudo
     CDS             2010..2450
                     /locus_tag="VLE22_24510"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          439 a          794 c          804 g          414 t
ORIGIN      
        1 cgtttcatca gggctaggaa ctgttgcgtc tgtacgttac tcagcctgac gccctgccgg
       61 accagttgat ccagattggt caacgtcgct ttgtccccat cgctccacgg tacgcgcctc
      121 agctgctcga tcaactctgc gaattcgcct gcccgccgcg gcccgaagag cggcgtcagc
      181 ctgcccgctg cccgcaacgc gccgccggat acgaagggca gggcggcgcc cactcccatc
      241 accgccagat cgcccacacc gagggtccgg ccccatcgat cttttccagc gagaagcccg
      301 taagcgaact cgccgatatc gaccgcgtcg ccgaggattg gtatcagtcc gacttccagg
      361 tcgatcagcg ccgtggcaag ctcctcccgg agcgttgcgt cacggtgagc gtatgccgga
      421 taccaggttc tggcatcgcg aatccgatcg atcgggaaag ttgggcgtgg aatcgtcgaa
      481 aatccacgcc ggggccgctc cggatggagc ggccaggcgc tgccgtcgga accttgcacc
      541 ggcacttgag aaaagtcttc gacccgaaat gcatcgcaaa gcccaagtgc gcgtactgtt
      601 tccggtggat cagctgcccg tcggggcccc tgcttccact tgaattcgac ggggcccgtc
      661 atcacaatct tcagcagagg ccgcaccgat ccgagcgcac cgtccgggtc tatgaaatac
      721 gcgaaacgcg ccttcgcgtc cgcgttgtcg tcctcgttcc aggtgagcgc agggacccgc
      781 atccggtaaa gcgttccggt atacggatgg cggatgtagg tgtcgctgta ggtgaccgag
      841 ggttctcgcc ggacgatgaa atttgccgga ccaggcatgc tggggatact ggcaaacggg
      901 ggcaccgtcg ccatccccag gggccggatt tccggcggcg gattgagttc ctcccgtccg
      961 caatacgcgg aggtggagac gccggtggcc atcggcggta tcacctcggg gtcctgcaca
     1021 cggatgacct cgaaagccgg cgtgtcgcga ctctgtatcc acgcaagcgg gaaactcttc
     1081 atcactacgg aaacgcccgg cgccgccacc acacggatga gcggatacgg ccgccgctgg
     1141 gtcggttcat cgcactgaaa ggcgaacgga tagcgccgtg gatttccgac gggaatcttg
     1201 atgagcagca gaatgactcg agccgtatcg ccggactggt acaccagccg aactccccgt
     1261 cccggactaa gctcctccgc cctgaactgg aacatcggtg cctattcctc cggctcctac
     1321 tggttcacac ggtccgccct ttcgctgcct cagatgctgc acagcatcga ttggaatgat
     1381 gccgtgatct tcggtcgctc tcgtgctgcg gccgccgacg cggtaacctc gatgactccg
     1441 ctggaggttg tagtacgccg gaatgcgcgc acgtgcgctc agcgtgggca cagaatgggc
     1501 acaaacgggg atcggagggt cctcgaggtg cgctgcaacc ccctggaaag aaatggtgga
     1561 cagggaggga gttgaacccc cgacaccggg attttcagtc ccgtgctcta ccaactgagc
     1621 tacctgtcca ctgggccgca ggccgcgtga agcgctggta accttaccag acgctccgag
     1681 gagtgtccag ccgactgggc cggctgacct ggagcccgtg tacctctggg tggacggggt
     1741 ctacgtcaaa gccgggctcg agaaggacaa ggccgtcatg ctcgtcgtcc tcgccggcct
     1801 gcgtgacggg cgcaagatcg tcctggccgt cgagagcggc tatcggaagt ccatcgagag
     1861 ttgggcggca tggctgcgag agctgaagca ccggggccgc cggcctcccc gtctcgtcgt
     1921 cggggatggc catttgggca tctggggcgc gctgaccgcc gtgttccccc cggcccgcca
     1981 gcagcgctgt tggataccac cggctcccga acgtgctcga caagctgccg aagcggctcc
     2041 aggcggaggg ccggcgccgg ctcgcggcga tcccctacgc cgagacgcgg gaggacgccg
     2101 aacgccagaa gcgggtgttc caagcgtggt gcacgaagaa gggcgtcagc gcagtcggcg
     2161 aggcgctcga ccgcgactgg gagcggctgg tcaccttcta cgaattcccg aaggcgcacg
     2221 cgaagcgcct gcgaacctcg aacccgatcg agtcgccgtt cgccgcggtc cgcttgcggg
     2281 cggcggccgc gaagcggtac aagaaggtcg agaacgcgac ggcggtcatc tcgaagacgc
     2341 tgctgctcgc cgagcggatg ttccggcggc tcgacgggcc cgaactgctg gccgaggtcg
     2401 cggaaggcgt gacgtatgtc aacggggtgc gcgcgaagga ccgcggctga c
//
LOCUS       DATIFQ010000483         4257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1887460, whole genome shotgun sequence.
ACCESSION   DATIFQ010000483 DATIFQ010000000
VERSION     DATIFQ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887460"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2488
                     /locus_tag="VLE22_24515"
     CDS             <1..2488
                     /locus_tag="VLE22_24515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17634.1"
                     /translation="FEYLQEVQVKTAGLEAEYGGALGGVVSAITKSGGNDFHGEIHYY
                     YYGNKISAGPTKRLQLDPVAEESVAYFQDQKNKRDFHEFGGSLGGPFIKNRLWFFTSV
                     SPRWHRATNEYLFDNGTEPGTMDREAFKWSWFNKVSFDPSSRVRMNFTWLYTPEYSTG
                     SLFAYDGFAPNVSTRSKATAEGQATRGYSQPEQSYTGNIDFTLSNTSLLSVKAGRYYL
                     DYKEVGIPYRYYTWWQASSIGIPGVPEELQKPFNFATPDAAQVLFDMTTRTYGQADFS
                     QFVRFIGQHNLKAGVGLQRNVNRLFDSWNGPDGRALLFWGLSFRGDTGTYGYYSVDDA
                     STKGSTGASITHLYIQDSWRIHRRLTLNLGLRTEKEIIPAFDRAFAKSFFADHRDYAF
                     KFGFGDKMSPRLGASFDVFGNGKLKISGAWGRYYDWTKFDLARGTFGADVWHVYYRSL
                     DTLDVYNLNLNNMPGRNLWTTGEFRNRRVPGFQYLDLEVKPMSADAMNFGVEYEIRPQ
                     MVFAGRYVHNHLNRTIEDMGALDAAGDEVYRYGNPGEGANTIFPSSGATCPIQVGEAC
                     GFKMPKAKRDYDAMELQLTRRFGGGWLANASYVYSRLWGNYSGLQSTDEIRPPTLGYG
                     FGLNQVFSAANYRPGGNANRYFDLDEAMWDAHGVVGLFGRLPTDRPHVFKFYGSRLFK
                     FGTEVGGFFRVMSGTPVTTQVVTINDIPFYVDGRGDHGRTPVVSQTDLLVAHEIKMGE
                     VKKLRFEFNMINLFNQKTSMFTFDRYNREEHSDSAGIDLSGTDLTQGFDWKAMVAATP
                     DGPKALDPRFGKAGIFNPGFQGRFLVKFVF"
     gene            complement(2585..2959)
                     /locus_tag="VLE22_24520"
     CDS             complement(2585..2959)
                     /locus_tag="VLE22_24520"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB17635.1"
                     /translation="MLAALQQFPTTSRSAPDRRANDRFPIVREVRYKVMNSKGSAEIG
                     SGETINISSTGVLFKAEKPLPPGKRLELSISWPAQLDGKCGLKLVARGRIVRCQGTNV
                     ALEIDKYEFRTAGRSLTTRAGI"
     gene            3113..3550
                     /locus_tag="VLE22_24525"
     CDS             3113..3550
                     /locus_tag="VLE22_24525"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSB17636.1"
                     /translation="MFRMRFIALAVGFTFLPLMLAAERKVIHPKEFKKGTPFSPAVLV
                     DGTLYVSGLVGQDLKTGKAPEEFEAEVKQCLENIGIILREAGMSYDDVVAVQVYLTDM
                     SLFPRMNSVYTTYFKEPRPTRTTVGVTQLAGPFRLEITVTARK"
     gene            complement(3534..>4257)
                     /locus_tag="VLE22_24530"
     CDS             complement(3534..>4257)
                     /locus_tag="VLE22_24530"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17637.1"
                     /translation="NGDARAAYNTLEAAVAASDGRVLSRQTVEDATQRKVLLYDKAGE
                     EHFNLISALHKSVRSSDPDAALYWLARMLEAGEDRLYIARRLIRMAIEDIGMADPRAL
                     EQAIAAMQTVHFLGIPEGDQALAQAALYLALAQKSDAAYRALNQAREKVQDTMADPVP
                     LHLRNPVTSSMRQWGYGEGYRHAHNFEDAVTDMDCLPESLAGTRFYTPTGRGLEKRIA
                     ERIEEIREAKHKKKAPNPTSGP"
BASE COUNT          839 a         1264 c         1229 g          925 t
ORIGIN      
        1 gttcgagtac cttcaggaag tccaggtgaa aaccgccggc ctggaggccg aatacggtgg
       61 agcgttgggt ggcgtagtca gcgcgatcac caagtccggc ggcaatgatt tccacggcga
      121 aatccactat tactattacg ggaacaagat cagtgcggga ccgacgaaac gtctccagct
      181 agacccggtg gctgaggaat cggttgcgta ctttcaggac caaaagaaca agcgcgactt
      241 ccatgaattc ggcggttcgc tgggtggacc gttcatcaag aacagactct ggttctttac
      301 gtccgtctct ccgcgctggc atcgcgccac gaacgagtac ctgttcgaca acgggactga
      361 gccgggtacg atggatcgcg aggccttcaa gtggagctgg ttcaacaagg tctccttcga
      421 cccgtcgagc cgcgtgcgga tgaacttcac ctggttgtac actccggagt actccacagg
      481 ctcgttgttc gcatacgatg gcttcgcgcc gaacgtctcc acgcggagca aggcgactgc
      541 tgaaggacag gctactcgcg ggtacagcca gcccgagcag agctacaccg gaaacatcga
      601 tttcacgctc agtaacacgt cgctgttgag tgtgaaggcc ggccgttatt acctcgacta
      661 taaggaagtc ggcatcccgt acaggtacta cacgtggtgg caggcgtcat cgatcggcat
      721 cccaggcgtt ccggaggagt tgcagaaacc cttcaacttc gccactccgg atgccgcgca
      781 ggttcttttt gacatgacga ctcgcacgta cgggcaggcg gatttcagcc agttcgtccg
      841 cttcatcgga cagcacaatc tcaaagcagg tgttggtctt cagaggaacg tcaacaggct
      901 gtttgattcc tggaacggtc cggatggccg cgctttgctt ttttggggcc tgtctttccg
      961 cggtgacacc ggaacatacg gttactactc ggtggatgac gcctcgacca agggatctac
     1021 cggagcgagc atcacccacc tgtacattca ggactcctgg aggatccatc ggcgcctgac
     1081 tctaaatctg ggtctgcgga ccgagaaaga gatcattcct gcgttcgacc gggccttcgc
     1141 caagtcgttc ttcgcagatc accgcgacta tgcgttcaag tttggctttg gcgataagat
     1201 gtctccacgc ctgggcgcaa gcttcgatgt cttcggcaac ggcaagctaa agatctcggg
     1261 tgcctggggt cgttattacg actggacgaa gtttgacctg gcgcgtggga cctttggcgc
     1321 cgacgtctgg cacgtgtatt accgctcgct cgacacgctc gacgtataca acctgaatct
     1381 gaacaacatg cccggccgga acctgtggac caccggagag ttccgcaacc gccgtgttcc
     1441 cggtttccag tacctcgatc tggaagtgaa gccgatgagc gctgacgcca tgaatttcgg
     1501 tgtggagtac gagatccggc cgcagatggt cttcgccggg cgctatgtcc acaaccatct
     1561 gaaccggacc atcgaggaca tgggcgccct cgatgccgca ggtgacgagg tgtaccgata
     1621 cggtaaccca ggcgaaggtg cgaataccat cttcccatcc tccggcgcca catgccccat
     1681 ccaggtcggc gaagcctgcg gcttcaagat gccgaaggcg aagcgtgatt acgacgcgat
     1741 ggagttgcag ctcacccgcc gttttggcgg cggatggctc gccaacgcca gctacgtcta
     1801 cagccggctt tggggcaact actcgggact tcagagcaca gatgagatcc gtccgcccac
     1861 gctgggctac ggcttcggtc tgaatcaggt cttcagcgca gcaaactacc gcccgggggg
     1921 taacgccaac cggtacttcg acctcgacga agcgatgtgg gatgcccacg gtgtcgtcgg
     1981 cttgttcgga cggctgccaa ctgatcgccc gcatgtgttc aaattctacg gttccaggct
     2041 gttcaagttc ggcaccgagg tcggcggctt tttccgcgta atgagtggca cgccggtgac
     2101 cacccaggtc gtcacgatca acgacattcc gttctatgtc gatggccgtg gagaccatgg
     2161 ccggacgccg gtggtcagcc agactgacct gcttgttgcc cacgaaatca agatgggcga
     2221 ggtgaagaaa ctccggttcg aattcaacat gatcaacctc ttcaaccaga agacaagcat
     2281 gtttacgttc gaccggtaca accgcgaaga gcactccgac tctgctggca tcgacctgag
     2341 cgggacagat ctcacgcaag ggttcgactg gaaagccatg gttgcggcga cgcccgatgg
     2401 cccgaaggcg cttgatcctc gcttcggcaa ggcggggatc ttcaaccccg gcttccaggg
     2461 ccggttcctc gtgaagtttg ttttctagcc cggaatcggg tcaagaaact caaaggacgg
     2521 ggccaaaagg ctccgtcctt tttttaatct ttgggctttt gtgatgtcgc cagaacagtg
     2581 gcggtcagat tcccgcccgt gtggtcaagc tccggccggc ggtccggaat tcgtacttgt
     2641 cgatctccag ggcgacgttt gttccctggc agcgtacaat gcggccgcga gccacgagtt
     2701 ttaggccaca cttcccatca agttgggccg gccaactgat ggaaagctcc agccgctttc
     2761 ccgggggcaa aggcttctcg gctttgaaca acacccctgt gctgctgatg ttgatcgtct
     2821 cgccgctacc aatttcggcg gagcccttcg aattcatgac cttgtaacga acttcacgga
     2881 cgatcgggaa ccggtcgttg gccctccgat ctggggcact tctcgatgtc gtcggaaatt
     2941 gttgtagtgc tgccagcatc cgctaaatcc tcttcgttct acttcatcct ccactttccc
     3001 gccgctctcg gcaataggaa aaggggttca ttgtgacggc atacccgata gtgctactgt
     3061 actccacctt tgttacgcta gttctacgtt tttttgagac tataatgacc ctatgtttcg
     3121 tatgagattc atcgctctcg ccgttggttt cacttttctg ccgctgatgc tcgccgcgga
     3181 acggaaagtc attcacccaa aagagtttaa gaagggtaca ccgttcagcc ccgcggtgct
     3241 cgtggacggc actctgtacg tctccggcct ggtcggccaa gacctgaaaa ccggtaaagc
     3301 cccggaggag ttcgaggccg aggtgaagca gtgtcttgag aacatcggga tcatcctccg
     3361 cgaagccgga atgtcctatg acgatgttgt cgcggtccag gtttacctga ccgacatgag
     3421 cctcttcccg cggatgaact ccgtctatac gacgtacttc aaagaaccgc gccccacgcg
     3481 cactacggtg ggtgtgaccc aattggccgg tcctttccgc ctcgagatta cggtcacggc
     3541 ccggaagtag ggttcggcgc cttcttcttg tgcttcgctt cgcggatctc ttcgatccgc
     3601 tccgcaatac gtttctcgag accgcggccc gtgggcgtgt aaaaccgtgt tccggcgagc
     3661 gattccggca ggcagtccat gtcggtcacc gcgtcttcga agttgtgcgc gtggcggtac
     3721 ccctccccat agccccactg ccgcattgac gaggtaacgg gattccgcag atgaagcgga
     3781 acgggatctg ccatcgtgtc ttgaactttc tcgcgggctt gattaagcgc ccggtaggcc
     3841 gcgtcggact tctgagcaag agcaagatag agcgccgctt gggccagcgc ctggtctccc
     3901 tccggaatgc caaggaaatg cacggtttgc atcgccgcta tagcctgttc gagcgcccgt
     3961 gggtcggcca tgccgatgtc ttcgatcgcc atgcggatca gccgccgggc gatgtacaaa
     4021 cggtcctccc ctgcttccag cattcgcgcc agccagtaca gagccgcgtc aggatcgctc
     4081 gaccggacag acttgtgcag cgcggaaatg aggttgaagt gctcctcgcc tgccttatcg
     4141 taaagcagca ctttgcgctg ggtggcgtcc tcgaccgtct gccgcgacag cacccgtcca
     4201 tcggaggcgg ccaccgcggc ttcgagcgtg ttgtacgccg cccgggcgtc tccgttg
//
LOCUS       DATIFQ010000484         2745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2268653, whole genome shotgun sequence.
ACCESSION   DATIFQ010000484 DATIFQ010000000
VERSION     DATIFQ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2268653"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2449
                     /locus_tag="VLE22_24535"
     CDS             <1..2449
                     /locus_tag="VLE22_24535"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSB17638.1"
                     /translation="LNHPNIITIHDIDQTDGIDFMSMELAAGRTLDKLIPRHGMRLND
                     ALKCAVQVADAVAAAHGAGIVHRDLKPGNVMVTDNGQVKILDFGLAKLTDAAEGSDDL
                     EMRTLRDGDAPRTVEGRIVGTVAYMSPEQAQGRKVDARSDIFSFGSLLYEMVTGHLPF
                     DADSNVSTLGAIIHKEPEPLGSKVPHDLEKVITRCLRKDPARRFQHMDDVKIALEELK
                     EESDSGIVRHDAPPKPTTGRRLLWWAVVPALLVLATAGVWFLRSRILMPEAPLAPVPL
                     TSYSGAEESPSFSPDGNQVVFSWNGPKEDNFDIYVKLIGGETLLRLTNTPAQEGHPAW
                     SPDGRSVAFLRDLGGRNEVLLIPAIGGPERKLAETRAWGSLAWFPDGKWLAFADKDTD
                     DAADERTILYAVSMETGDRRRLTSPPRDGWDFNGTFSPDGRYLVFSRGTAYWVSELFL
                     LPLRADLSPGGEPEQITFQRRFTHGPVWTPDGREIVYGSGTATANEMWTIPVSGSRTT
                     RRLEFAGDQVLNPTISRQGHRLAYARRSTDRNIWQMELRGPGGNPGNPVSLVSSTWSD
                     HAPQYSPDGSKVVFISGRSGNNEVWVCGKDGSNPIQLTSMRAQETGAPQWSPDGKRIV
                     FDSNAGGNFELYTIEAGGGRQQRLTNHPADDAVASFSRDGNWIYFASSRTGRFEIWKM
                     AANGGDPVQITKNGGWRALESADGKQLYYAPNRRVSSLWRLPLDGGKEQQITEPVVGS
                     ALAVTRTGLYFARPSRPGGGNPIEFLSFATGKIIAVATTARPIMWALSVSPDERRLIY
                     DQVDQTGSDLMLVENFR"
     gene            2454..>2745
                     /locus_tag="VLE22_24540"
     CDS             2454..>2745
                     /locus_tag="VLE22_24540"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB17639.1"
                     /translation="MNSGAQSARVVRFGLFEADLMAGELRKCGRKVELQDQPFHVLAL
                     LLRRPGELVTREELRQALWPADTFVEFDESLNKAVQKLRQALGDSADNPRFIE"
BASE COUNT          543 a          820 c          896 g          486 t
ORIGIN      
        1 actgaatcat ccgaacatca tcacgatcca tgacatcgac cagaccgacg ggatcgattt
       61 catgtcgatg gaattggccg ccggcaggac gctggacaag ctgatcccgc ggcacgggat
      121 gcggctgaac gatgcgctca agtgcgccgt gcaggtcgcc gacgcggtgg cggccgccca
      181 cggagccggc atcgtacacc gcgacctgaa gcccggaaac gtcatggtga cggacaacgg
      241 ccaggtcaag atactcgact tcgggctagc caagctgacg gacgcggccg agggcagcga
      301 cgaccttgaa atgcgaacac tccgtgacgg ggacgcgccg cgcaccgtgg aaggcaggat
      361 cgtgggtacg gtcgcctaca tgtccccgga gcaggcgcaa ggcaggaagg ttgacgcgag
      421 gtcggacatc ttcagtttcg gttcgttgtt gtacgagatg gtgacaggcc acctgccgtt
      481 cgatgccgat tcgaatgtgt ccacgctggg ggcgatcatc cacaaagaac cggagccgtt
      541 gggatcgaaa gtacctcacg atctggagaa ggtgatcaca cgctgcctcc gcaaagaccc
      601 ggcgcggcgc ttccagcaca tggacgacgt gaaaatcgcg ctggaagaac tgaaggagga
      661 gtccgactcg ggcatcgtgc ggcacgacgc gccgccgaag ccgacaactg gccgccggct
      721 gctgtggtgg gccgtggtgc ccgccttgtt ggtccttgcg acagcgggag tatggtttct
      781 tcgctccagg atcttgatgc cggaggcgcc gctggccccc gtccccctca ccagctattc
      841 gggggcggaa gagtccccca gtttttcccc ggacggcaac caggtcgtat ttagctggaa
      901 cgggccgaag gaggacaact tcgacatcta tgtcaagctg atcggcggcg agactttgct
      961 acggctcaca aatactccgg ctcaagaggg acatcccgcc tggtcaccgg acggacggag
     1021 tgtggcgttt cttcgcgatc tcggtggccg aaatgaggta ctgctgatcc cggccatcgg
     1081 cgggccggag cgcaaactcg ctgagacccg tgcatggggc tcgctggcgt ggttccccga
     1141 cggtaaatgg cttgcgttcg ccgacaagga tactgacgac gctgccgacg aacgcacgat
     1201 cctgtatgca gtctccatgg aaaccggcga ccggcgcaga ttgacgtccc ccccacgaga
     1261 tggctgggac ttcaatggga ccttttctcc cgacggccgt tacctggtgt tcagccgggg
     1321 taccgcgtac tgggtcagtg aactcttcct gctgccgctt cgcgccgatc tcagccccgg
     1381 cggcgaaccg gagcagatca cgttccagcg gcgttttaca cacggtccag tctggacacc
     1441 ggatggacgc gaaatcgttt atggcagcgg caccgccact gcgaacgaga tgtggacgat
     1501 tccggtgtcc ggctcgcgga caacacgccg gctcgaattc gccggagatc aagtcctaaa
     1561 cccgacgatt tccagacagg gccaccgcct ggcgtacgca cgccggtcaa ccgaccgcaa
     1621 catctggcag atggaactgc gcggtccggg aggcaacccc ggcaaccccg tgagcctggt
     1681 ttcgtcaacg tggtccgacc acgcgcccca gtactcgccg gatgggagca aggttgtgtt
     1741 catctccggg cgttcgggaa acaacgaagt ctgggtctgt gggaaggacg gctcgaatcc
     1801 catacagttg acctccatgc gggcgcagga gaccggcgca ccgcagtggt ctcccgatgg
     1861 aaagcggatc gtctttgact ctaacgccgg tgggaacttc gagctttata cgatcgaggc
     1921 gggcggcggc agacagcagc ggctgacgaa ccatcctgcc gacgatgcag ttgcgagctt
     1981 ctcgcgagac gggaactgga tctactttgc ctcgtcgcgg accgggcgct tcgagatctg
     2041 gaagatggcg gcgaacggcg gggatccggt ccagatcaca aagaacggcg gatggcgggc
     2101 gctcgagtcg gctgacggca agcaactgta ttacgccccc aatcgaaggg tgagcagttt
     2161 gtggagactt ccgttggacg gaggcaaaga gcaacagatc acggagccgg tggtaggcag
     2221 cgcactcgcg gttacgagga cgggcctcta cttcgcccgg ccgtctcgcc cgggtggagg
     2281 caatcccatc gagttcctga gcttcgcgac cggcaagatt atagccgtgg cgacaactgc
     2341 gcggcctatt atgtgggccc tgtccgtttc cccggacgaa cgccgcctca tttacgacca
     2401 agtcgatcag acgggtagcg acctgatgct ggtggagaac ttccggtgag gctatgaaca
     2461 gtggcgcaca gtccgcgcgg gtcgttcgct tcggattgtt tgaagcggat ctcatggccg
     2521 gggaactgcg caagtgcggg cggaaggtcg agcttcagga tcagcctttt catgtcctgg
     2581 ctctgttgct gcggcgtccg ggggagttgg tcacgcgcga ggaactgcgc caagcgctgt
     2641 ggccagccga caccttcgtc gagttcgatg agagcctcaa caaggccgtc cagaagctcc
     2701 ggcaggcact gggcgactcg gcagacaatc cccgtttcat tgaaa
//
LOCUS       DATIFQ010000485         9930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2845468, whole genome shotgun sequence.
ACCESSION   DATIFQ010000485 DATIFQ010000000
VERSION     DATIFQ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2845468"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VLE22_24545"
     CDS             <1..209
                     /locus_tag="VLE22_24545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17640.1"
                     /translation="LHFNLHAALMPGFCLMWDLADLVKAVAPRKVLWTDPTDWMGNVI
                     HVDGDYRYRPFETPDTPYIDTLLR"
     gene            complement(272..1558)
                     /locus_tag="VLE22_24550"
     CDS             complement(272..1558)
                     /locus_tag="VLE22_24550"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HSB17641.1"
                     /translation="MENETLTITDNRTGKTYSVQLFKGAVRSMDLRQIKTETDEFGLM
                     CYDPAFMNTASCQSSITYIDGDKGILRYRGYPIDVLAEKCSFLETAYLVLNGELPNKS
                     QLDEFTAEITRHTMLHENTKKLMEGFHYDAHPMGMLVSSVAALSTFYPEAKNVHDPAI
                     RHLETIRLIAKMATIAAHCYRHNLGLPYVYPDNSLSYTENFMSMLWKRAEPKYEPNPV
                     LARALEILFILHMDHEQNCSTNAMRAVGSSLADPYVTTAAAIAALYGPLHGGANEQVL
                     RMLDEIGSVENVPAFVDKIKAGKGRLMGFGHRVYKNYDPRAKIIKEAAEAVFGVTGKN
                     PKLEIALELERVALSDEYFIKRKLYPNVDFYSGIIYQAMGFTTEMFTVLFAIPRTVGW
                     LAQWQELLQDSEQKIARPRQIFTGHWLRDFVPMEKR"
     gene            complement(1734..1952)
                     /locus_tag="VLE22_24555"
     CDS             complement(1734..1952)
                     /locus_tag="VLE22_24555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17642.1"
                     /translation="MSEQKHYIPIWFFVGALLLFYGVLITGAGIYYLFAPMPNEVVLA
                     HLHAPIWWGGLLTALGAFYTIHFRPGKQ"
     gene            complement(1959..3680)
                     /locus_tag="VLE22_24560"
     CDS             complement(1959..3680)
                     /locus_tag="VLE22_24560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSB17643.1"
                     /translation="MTLLFLPSFWLQATQSRLLTMSPVDLVIVVAYFVLVLVIGFYLK
                     RYASTGEDFFLAGRQMTASVAGLSFISANLGSLETMGYAAATYQHGMVIAHAYFIAAI
                     PAILFLAIVMMPFYYISKTHSVPGYLKLRYGESTRSISSITFAVMTILMSGINMFSMA
                     KVMQVILGWDFNYSMWVSSLTVAVYVMMGGLLSAIFNEVLQFFLIWFGSLLIPILGLI
                     EAGGWDNMVARINQRFAGQDYTHLWRNMGSFTDNPMGLDWIGVVLGWGIAISFGYWTT
                     DFLVVQRVLAAKNLRSAQLGTILGAALKMCVPLIVILPGLLGLAVLPMNLVSEPEALR
                     TGQHSYNEVLPLMMARYCGPGLLGLGVTALIAGFMSGMAGNVSAFATVWTYDIYKALI
                     RKTASDEHYVKMGRWCSILGVFASIGTAYLVMFFDNIIAYAQVIFIFGIVPQFGTIIL
                     GMLWKRVTPKGGFYGLLAGTLTSIGLFAWVRLDRSALKYVAFYENASEMADNIYRAFW
                     CLLATFVVTIVVSLLTKPKPDSELENLVYGLTPIPKEKGLPWIQRPAFWAAVVGAAFV
                     MVNIIFW"
     gene            complement(3786..3974)
                     /locus_tag="VLE22_24565"
     CDS             complement(3786..3974)
                     /locus_tag="VLE22_24565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17644.1"
                     /translation="MLRTWKRVREWPFWRETEGQDLIEYALMAGFVAVAGGAAFPPVA
                     TSLSTIFSKVGSVLNAAG"
     gene            complement(4097..6256)
                     /locus_tag="VLE22_24570"
     CDS             complement(4097..6256)
                     /locus_tag="VLE22_24570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17645.1"
                     /translation="MRVRFGTAVLALIALCTAAGGAPSLTTIEDVLYKADGSAFSGVV
                     FIEWRSFQAADSSNIATHSLTVPIANGVLRVHLVPTTNALSGAYYSARYYSDGRIQFD
                     EKWSVPPSAAPLRLKDVRITGSVAPGTALPPATQTQIQESDVVGLVADLAARPLKGPE
                     YAPSRAAYINESGMLDAIAGNLTDCVRVDGTAGPCDVNASTGPGFVDGETPAGLVSGS
                     NAVFTLSEIPSPPSSLTLYRNGVRQKADLDYTLSGNTITFAGASIPQAGDVLLCSYRL
                     ADAGNPAGAAGGALTGTYPSPQIAAGVISNINVSDVAAIAESKLALNYPTHSGANDPT
                     AEQKAAMAGTSGTPSAGNRYVTDQDPRMTNPRTASAHALLGNVHNDTTQGTPVRGDII
                     VAAGGSPAMWTRIPIGPANRCLMSNGFDAVWNSCLYTGFAAGSVPFVDAAGNLAQNNA
                     RLFWENNNRRLSVGNNSSLATLYVWDSLASTGLTGLVVRAGQGQGSTPLQTWLDPNGT
                     ELARVDVAGNFSGASFRGATTGTKAAWQDAGNSADPGTRTDGDFWYNSGAQARKTVEG
                     GQTHTVPQVLCANAGTAVSGPSLTRLGSCTIPANFLKPGDRVEVRFSYSHEGAARSFT
                     FEVRWGGTTLLSRAGAATETLVAGHAEAGIHAGGAQWEVQSWGASLAPAWGVGNAGDS
                     LASTLVLDFLGSMASATTDTLTLRNFTVVRYPAQANP"
     gene            complement(6460..7743)
                     /gene="serS"
                     /locus_tag="VLE22_24575"
     CDS             complement(6460..7743)
                     /gene="serS"
                     /locus_tag="VLE22_24575"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HSB17646.1"
                     /translation="MHDLAYFRANLDEIAQRLATRGFALDVKQCQDLDARRRSAITEA
                     EQLKAKRNAESAEIAKLRKQGVDTAEHQQKVREIGTRISELDEQVKGLDEEFREMLAG
                     IPNVPHDSVPEGSSEAGNAEVRRWGQAREFDFEPKAHWDLGPELGVLDFERAAKITGA
                     RFAVYWGLGARLERALFNFMLDVHTREHGYTEVFPPYMINSASLFGTGQLPKFAEDLF
                     KCENYDLWLAPTAEVPVTNLYREETIDADRLPVRLCAFTSCFRSEAGSYGKDVRGIIR
                     QHQFQKVELVKFTRPEQSYDELEALTRDAEDILQRLGLPYRTVALCTGDLGFSSAKTY
                     DIEVWLPGVSTFREISSCSNFEGFQARRASIRAKAGKGKAEFVHTLNGSGLAVGRTWV
                     AVVENYQQKDGSVIIPEALRPYMNAERITKTGDMR"
     gene            7862..9064
                     /locus_tag="VLE22_24580"
     CDS             7862..9064
                     /locus_tag="VLE22_24580"
                     /inference="COORDINATES: protein motif:HMM:NF037031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HSB17647.1"
                     /translation="MLFLLAVITITAAFDGGSVGEVKQVAPAHLRCGVKGQADQNNRN
                     RQANWYYFRLDSLPREQVRIDLVNLAGEYNFRPGALSVTRNSRPVYSYDGERWIHFTD
                     AQVEWDNAEPRLTLRFTPERPTMWIAHVPPYTNRQLTRLFDDFRRSPNLRREVIGHSV
                     NGREIPLLTVTDASVPRERKDVVWLMARQHAWEAGTSWIAEGALRFLLSDSPEAAKLR
                     RSVIWKILPMMDPDGVASGAVRFNANGYDVNRNWDAVDERLMPEITAARRAIFAWLDS
                     GRQIDLFLALHNTESGDFIEGPLAQGREPVRALASRFADALARTTASFHATGPPRDAG
                     LSTTPGMKGRMTVNQALFHERQIPAFLMETMVDRNPKLGRPRTTEDNLTFGSELVRAM
                     AASLPPAR"
     gene            9102..9896
                     /locus_tag="VLE22_24585"
     CDS             9102..9896
                     /locus_tag="VLE22_24585"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HSB17648.1"
                     /translation="MKYRYEEFTWPEIREAVAQRRVPVLPVGTVEQHGPHLPLVTDVL
                     CASEMARRAVERIPYEAMLLPPVYYAFNEHHLDFPGTIAIEGPTFINYVTDIGKSLAH
                     HGFRKILLVNGHGSNVPFLDVAARNITNHSDAICAMVPWWNLIPKELFGELRESEWPG
                     GMAHACELETSVLLYLRGDLVQIDLAQRDFPGQHSEFFYWDLQKASPVYFQEFFSRYS
                     RTGTLGDPTKATVEKGRRFVDAVADRLVALIQDFRGHTIEPRVDHH"
BASE COUNT         1811 a         3448 c         2781 g         1890 t
ORIGIN      
        1 cgctgcactt caacctccac gctgctctga tgccgggctt ctgcctgatg tgggacctcg
       61 cggacctggt gaaagcggtc gcgccgcgca aggtcctctg gactgacccg acggactgga
      121 tgggcaacgt tatccacgtc gacggcgact accgctaccg ccccttcgag acgcccgaca
      181 ccccgtacat cgacacactg ctgcgctaac ccaccgcaaa aacgcgggag cggccccttg
      241 ccgccgcccc ggccgcaatc cggcgtgcgt actaccgctt ttccatcgga acgaagtcgc
      301 gcagccagtg gccggtgaag atctggcgcg ggcgggcgat cttctgctcg gaatcctgga
      361 gcagctcctg ccactgcgcc agccagccga ccgtacgcgg aatcgcgaac agcaccgtga
      421 acatctcggt ggtgaatccc atcgcctggt aaatgatgcc cgagtagaaa tccacgttcg
      481 ggtacaactt gcgcttgatg aagtactcat ccgacagcgc cacccgctcc agttcgagcg
      541 cgatctcgag cttcgggttc ttccccgtga ccccgaacac cgcctcggcc gcctctttga
      601 tgatcttcgc gcgcgggtcg tagttcttgt acacccggtg gccgaatccc atcaggcggc
      661 cttttccggc cttgattttg tccacgaacg ccggcacgtt ctccaccgac ccgatctcgt
      721 cgagcatacg cagcacctgc tcgttcgctc cgccgtgcag cgggccgtac agggccgcga
      781 tcgccgccgc cgtggtcaca tacgggtccg ccagcgagct tcccaccgcc cgcatcgcgt
      841 tggtgctgca attctgctcg tgatccatgt gaaggatgaa gaggatctcg agcgcgcggg
      901 ccagcaccgg attcggctcg tacttcggct ccgcccgctt ccacaacatg ctcatgaaat
      961 tctcggtata gctgagcgag ttgtcgggat agacgtacgg cagcccgagg ttgtgtcggt
     1021 aacagtgagc ggcgatggtt gccatcttcg caatcagccg gatcgtctcg agatgccgga
     1081 tcgccgggtc atgcacgttc ttcgcttcgg gatagaacgt cgacagcgcc gccaccgagc
     1141 tgaccagcat tcccatcgga tgggcgtcgt agtggaagcc ctccatgagc ttcttcgtgt
     1201 tctcgtgcag catcgtgtgc cgcgtgatct cggcggtgaa ctcgtcgagc tggctcttgt
     1261 tcggcaattc gccgttcaga accaggtatg cggtttcaag gaagctgcac ttctccgcca
     1321 gaacgtcgat gggataaccc cgataacgta ggattccctt atcgccatca atataggtaa
     1381 tactgctctg gcaggaggcc gtgttcatga acgccgggtc gtagcacatc aacccgaatt
     1441 cgtcagtctc cgtcttgatc tgacgcaggt ccatcgaccg gacggcgccc ttaaacaact
     1501 ggacactgta cgtcttcccg gtccgattgt cggtaatcgt aagggtctca ttctccatca
     1561 acgtgtctcc tggctcaatc ccctttgccg cccagtggca atccacgtgc caaacgccgt
     1621 tccgccgctc cggatgaagt atccctcaag tttccgacgc tcccggttaa agatcaaggg
     1681 cgcagccggg tcatttggcg cagccctggg cacccgattg gggacggacc gccctactgc
     1741 ttccccggcc ggaaatgaat cgtgtaaaag gcccccaggg ccgtcagcag ccccccccac
     1801 cagatcggcg catgcagatg cgcgagcacg acctcgttcg gcatcggcgc gaacagatag
     1861 tagatgcccg cgcccgtgat cagcacgcca taaaacaaca gtagcgcacc cacgaagaac
     1921 cagatcggga tgtagtgttt ttgctcgctc atgggcccct accagaaaat gatgttcacc
     1981 atcacgaaag cggcgccgac caccgccgcc cagaaggcgg gccgctggat ccacggcagt
     2041 cccttttctt tcgggatggg cgtcaagccg tagacgaggt tttcgagctc cgaatccggc
     2101 ttaggcttcg tgagcaggct caccacgatg gtcactacaa aggttgccag caggcaccag
     2161 aacgcccggt agatgttgtc cgccatttcg ctggcgttct catagaaggc cacgtacttc
     2221 agcgccgagc ggtcgagcct tacccatgcg aacagcccga tcgacgtcag cgtgcccgcc
     2281 agcaggccgt agaagcctcc cttcggcgtc acccgcttcc acagcattcc gaggatgatc
     2341 gtcccgaact gcggcacgat tccgaaaatg aagatcacct gcgcgtaagc gatgatgttg
     2401 tcgaaaaaca tcaccaggta cgccgtgccg atgctcgcga acacgccgag gatcgagcac
     2461 caacgcccca tcttcacgta atgctcgtcg ctcgccgtct tgcgaatcag cgccttgtaa
     2521 atgtcgtacg tccacaccgt cgcgaacgcg ctcacgttgc cggccattcc ggacatgaac
     2581 ccggcgatga gcgcggtcac gcccaagccg agcagtcccg gcccgcaata gcgcgccatc
     2641 atcaacggca gcacttcgtt gtaactgtgc tggcccgtcc gcagcgcctc cggctcgctc
     2701 accagattca tcggcagcac ggccaagccc agcagtccgg ggagaatgac gatcagaggc
     2761 acgcacatct tcagcgccgc gccgagaatc gtccccaatt gcgccgaccg gaggttcttg
     2821 gcggccagca cgcgctgcac caccagaaaa tccgtcgtcc agtacccgaa tgagatcgca
     2881 atgccccatc ccagcactac accgatccag tcgaggccca tcgggttgtc cgtgaacgaa
     2941 cccatgttcc gccacaagtg cgtgtagtcc tggccggcga aacgctggtt gatgcgcgcc
     3001 accatgttgt cccagccgcc agcctcaatg agccccagaa tcggaatcag cagcgagccg
     3061 aaccagatca ggaagaattg gagtacctcg ttgaagatcg ccgagaggag cccgcccatc
     3121 atcacataca ccgcgaccgt aagcgagctg acccacatgc tgtaattgaa atcccagccg
     3181 aggatcacct gcatcacttt cgccatcgag aacatgttga tcccgctcat caggatcgtc
     3241 atcaccgcga acgtgatgct cgaaatcgac cgcgtcgatt cgccgtagcg cagtttcagg
     3301 tatcccggga cggagtgcgt cttcgagatg tagtagaacg gcatcatcac gatggcgagg
     3361 aacaggatgg caggaatcgc cgcgatgaag taggcgtgcg cgatcaccat gccgtgctgg
     3421 taggtcgccg ccgcgtaccc catcgtctcc agcgatccca ggttggcgga aatgaagctg
     3481 aggccggcca ctgaggccgt catttgccgg ccggcgagaa agaagtcctc tcccgtgctc
     3541 gcgtaccgtt tcaggtagaa cccgatcacg agcaccagaa cgaagtaggc gacgacgata
     3601 accaggtcca ccgggctcat cgtcagcagc cgggattgcg tggcctgaag ccagaaggaa
     3661 ggtaagaata gcagggtcat gacggcagtt cgaccagggt cgcgtatcga gtcacgaacg
     3721 actcattgta tacgcgcgtc ccccgcctgt cgatgccgtg atgtggagcg ggtgtggcag
     3781 gtcggtcaac ctgccgcgtt cagtaccgat cccactttcg agaaaatcgt gctgagcgaa
     3841 gtcgccaccg gcgggaacgc cgctccccct gccacggcca caaatcccgc catcagcgcg
     3901 tactcgatca ggtcctggcc ttcggtttca cgccagaacg gccactccct gactcgtttc
     3961 caggttctca acatgggttc ctccctcgtg aactgcgctt ccgtcctccc catgctggcc
     4021 gacggcgcac cggcccacaa tgggagcaaa acccaataaa acccggctaa acctcttaac
     4081 cctctgtggt gaactcttat ggattcgcct gcgccggata ccgcacaacc gtgaagtttc
     4141 gcaaggtgag cgtgtccgtc gtggcactgg ccatgctgcc gaggaaatcc aacaccagag
     4201 tcgacgccag cgaatctccg gcgttcccga caccccacgc cggcgccagc gaggcccccc
     4261 agctttggac ctcccattgc gccccgcccg cgtggatccc cgcctcggcg tgccccgcta
     4321 ccagcgtctc cgttgccgcc cccgcgcgcg agagaagcgt cgtcccgccc cagcgcacct
     4381 caaacgtgaa gctacgcgcc gcgccttcgt gcgagtagct gaagcggacc tcgacccggt
     4441 ctcccggttt caggaagttt gccggaatcg tgcagcttcc caaacgggtc aagcttggcc
     4501 cactgactgc cgtgcccgcg ttagcgcaca gtacttgcgg aacggtgtgt gtctggccgc
     4561 cctccaccgt cttccgcgcc tgggcgccgc tgttgtacca gaagtcgccg tcggtgcgag
     4621 tgcccggatc cgcggagttg cccgcatcct gccaggctgc tttagtgcct gttgtcgccc
     4681 ctcggaacga agccccggaa aagttgcccg ccacgtccac ccgcgccagt tctgtcccgt
     4741 ttggatcgag ccacgtttgc agcggagtcg acccctgtcc ctggcccgcg cgcactacca
     4801 gcccggtcag tccagtcgac gcgagcgagt cccagacgta tagcgtcgcc aggctggagt
     4861 tgttgcccac cgacagccgc cggttgttgt tctcccagaa gaggcgggcg ttgttctgcg
     4921 caaggttccc ggctgcgtcg acgaacggca ccgagcccgc cgcgaatccc gtatacaggc
     4981 acgagttcca gaccgcgtca aaaccgttcg acatcaggca ccggttcgcc ggcccgatcg
     5041 gtatccgcgt ccacatcgcc ggtgagcccc cagccgcgac gatgatgtca cctcgcaccg
     5101 gcgtcccctg cgtcgtatcg ttgtgaacgt tccccagcaa cgcgtgggcc gatgccgtcc
     5161 gcgggttcgt catcctggga tcctgatccg tcacgtaccg gtttcccgcc gacggcgtcc
     5221 cagacgtgcc tgccatcgcg gccttttgtt ccgctgtcgg atcattggcg ccggagtgcg
     5281 tcggataatt cagcgccagc ttcgactcgg cgatcgctgc cacgtcgctc acgttgatgt
     5341 tggagatcac accggccgcg atctgcggac tcggatacgt tcccgtcagc gcgccacccg
     5401 ctgcgccggc cgggttcccg gcgtcggcca agcgatagga gcagagcagc acgtcgcccg
     5461 cctgcgggat cgacgcgccg gcgaacgtga tcgtgttccc gctgagcgta tagtcgaggt
     5521 cggccttctg acgcacgccg ttcctgtaca gcgtcaggct cgatggcggg cttgggattt
     5581 cgctcagtgt gaacaccgcg ttcgacccgc tcaccaagcc cgccggcgtc tcgccgtcca
     5641 cgaatcccgg gccggtgctc gcgttcacgt cgcacggacc cgcggtgccg tccacccgca
     5701 cgcagtcggt cagattccca gcgatggcgt ccagcattcc cgactcgttg atgtacgcgg
     5761 cccgcgacgg cgcgtactcc ggccccttta gcggccgcgc agccaagtcg gccaccaggc
     5821 cgaccacatc cgactcctga atctgcgtct gcgttgccgg cgggagcgcc gtgcccggcg
     5881 caaccgagcc cgtaatccgg acatccttca gcctcagcgg cgctgcgctc ggtggcaccg
     5941 accatttttc gtcgaactga attcgcccgt cgctgtaata ccgcgccgag tagtacgcgc
     6001 ccgacagcgc attcgtcgtc ggcacaaggt gcacccgcaa cactccgttc gcgatcggaa
     6061 ccgtcaggct gtgcgttgcg atgttcgacg aatcggccgc ctggaaactc ctccactcga
     6121 tgaacaccac tccgctgaac gccgacccgt ccgctttgta cagcacgtcc tcgatggttg
     6181 tcagcgatgg tgccccgccc gccgccgtgc agagcgcaat caacgccagc accgccgtcc
     6241 cgaatcgaac ccgcatcctc ggcctcccga aaaaaagccc ggcccgcact tggcggaccg
     6301 ggccggctga atctcagcct ttcggttcca ctctatgcat gagttctccg ggatacacgc
     6361 cgaaacacgg cgccacgaga caataactca ctgtaacatc aacgagttat ctatttgttc
     6421 ttggtctgtg actcgaactt tgcagcttcc ggggaaacct cagcgcatat cacccgtttt
     6481 cgtgatccgc tctgcgttca tgtatggtcg aagcgcctcg ggaataatca cgcttccgtc
     6541 cttctgctgg tagttctcga ccaccgccac ccacgtgcgt ccgaccgcca gcccgcttcc
     6601 gttcagcgtg tgtacgaact cggccttgcc cttgccggcc ttcgctcgga tgctcgcccg
     6661 ccgcgcttga aagccctcga agttcgagca ggacgagatc tcgcggaagg tgctcactcc
     6721 cggcagccat acctcgatgt cgtaggtctt cgccgacgag aatccgaggt cgcccgtaca
     6781 cagcgccacc gttcgatacg gcagtccgag cctctggagg atgtcctcgg catcgcgtgt
     6841 cagtgcctcc agttcgtcgt agctctgctc cggccgggtg aacttcacca gttcgacctt
     6901 ctgaaactgg tgctggcgga ttatgccccg cacgtctttt ccgtacgagc ccgcctcgct
     6961 ccggaagcag gacgtaaacg cgcacaggcg gaccggcaac cggtcggcgt caattgtctc
     7021 ctcgcggtac aggttcgtca ccggcacctc ggccgtcggc gcgagccaga ggtcgtagtt
     7081 ctcgcacttg aacaggtcct cggcgaactt cggcaactga cctgtcccga acaggctcgc
     7141 cgagttgatc atgtacggcg gaaacacttc tgtgtagccg tgctcccgcg tatgcacgtc
     7201 gagcatgaag ttgaacaggg cccgctcgag ccgcgccccc agcccccagt agacggcgaa
     7261 ccgcgcgccg gtgattttcg ccgcccgctc aaaatccagc acgcccaatt ccgggccgag
     7321 gtcccagtgc gccttcggct cgaaatcgaa ctctctcgcc tggccccacc gccgcacttc
     7381 ggcattgccc gcttcgctgc tgccctccgg taccgagtcg tgcggaacgt tgggaatgcc
     7441 ggccagcatc tcgcggaact cctcgtcgag ccctttcacc tgctcgtcca actccgagat
     7501 ccgcgtaccg atttcccgca ccttctgctg gtgctccgcg gtgtcgaccc cctgtttgcg
     7561 cagtttcgcg atctccgcgc tctccgcgtt gcgcttcgct ttgagttgtt ccgcctccgt
     7621 gatcgccgaa cgccgccgcg catcgaggtc ctgacactgc ttcacatcga gagcgaaccc
     7681 gcgcgttgcg agccgctgcg ctatctcatc gaggttcgcc cgaaaatacg ccagatcgtg
     7741 catctttcta gtttagagag gttccgatgc gaaaaacggg gactggctcg aatttcgcgg
     7801 tgtttgcgaa attggtgcct gtccccgctt ttcgtgtctt cagctatcct gttctgttcc
     7861 catgctcttt cttctcgccg tcatcaccat caccgccgcg ttcgacggcg gcagcgtcgg
     7921 cgaagtgaaa caggtcgccc cggcacacct ccgctgcggc gtgaaaggtc aggccgacca
     7981 gaacaaccgc aaccgccagg ccaactggta ctactttcgc ctcgacagcc tgccgcggga
     8041 gcaggtccgt atcgacctgg tgaatctggc cggcgagtat aacttccggc ccggcgcctt
     8101 gtccgtcacc cggaactcgc gcccggtcta cagctacgac ggcgagcgct ggatccactt
     8161 caccgacgcg caggtcgaat gggacaacgc cgagccccgc ctcacgctgc gttttacccc
     8221 ggagcgcccg accatgtgga tcgctcacgt cccgccgtac acaaaccgcc agctcacacg
     8281 ccttttcgac gactttcggc gtagcccgaa cctccgccgc gaagtcatcg ggcacagcgt
     8341 caacggccgc gagattcctc tgctcaccgt gaccgatgcg tcggtcccgc gggagcggaa
     8401 ggatgtcgtg tggctaatgg cgcgtcagca cgcctgggaa gcgggcacgt cgtggatcgc
     8461 cgagggcgca ttgcgttttc tcctctcgga ttcacccgag gcggcgaagc tccgccgctc
     8521 ggtcatctgg aagatccttc cgatgatgga tcccgacggc gtcgcctccg gggccgttcg
     8581 ttttaacgcg aatgggtacg acgtcaatcg caactgggac gccgtcgatg aacgcctcat
     8641 gcccgagatc accgccgccc gccgcgcgat cttcgcgtgg ttggactccg gccgccaaat
     8701 cgaccttttt ctcgccctcc acaacaccga atcgggagac ttcatagaag gccccctcgc
     8761 gcagggcaga gagcctgtcc gcgctctggc gtctcgattt gcggacgcgc tggcgcgcac
     8821 caccgcatcg ttccacgcca ccgggccgcc gcgagacgcc ggactctcca ccacccccgg
     8881 catgaaaggc cgcatgaccg tcaaccaggc tcttttccac gagcgacaga ttcccgcttt
     8941 cctcatggag acaatggttg accgcaaccc gaaactcggc cgcccgcgca ccaccgaaga
     9001 taacctgacg ttcggcagcg aactcgtgcg cgcgatggct gcgtccctcc ctccggcccg
     9061 gtgaggacca ccgattccgg tacaatggct ccaatcgtcc gatgaagtat cgttacgaag
     9121 agttcacgtg gccggagatc cgcgaggccg tcgcgcagcg ccgggttccg gttctgcctg
     9181 ttggcacggt tgagcagcac ggtccgcacc tgcccctcgt caccgacgtg ctctgcgctt
     9241 cggaaatggc ccgccgcgcc gtcgagcgga ttccttacga agcgatgctc ctgccgcccg
     9301 tttattacgc cttcaacgag caccacctcg attttcccgg taccattgcg atcgaaggcc
     9361 ccacgttcat taattacgtc accgacatcg gaaagagttt ggcccaccac ggctttcgca
     9421 agatcctcct cgtcaacggc cacggcagca atgtcccgtt cctcgacgtc gctgcccgca
     9481 acatcaccaa ccactccgac gctatttgcg ccatggttcc ttggtggaac ttgatcccga
     9541 aggaactatt cggcgagctg cgtgaatccg aatggcccgg cggcatggcc cacgcctgcg
     9601 aactggagac ctccgtactg ctatacctcc gcggcgatct cgtccagata gacctcgcgc
     9661 agcgcgactt ccctgggcag cattccgagt ttttctactg ggacttacag aaagcctcac
     9721 cggtgtactt ccaggagttt ttcagtcgct attcgcgcac cggcacgctc ggcgatccca
     9781 ccaaagccac cgtcgaaaag ggacgccgct tcgtcgatgc cgtagccgac cgcctcgtcg
     9841 cgctcatcca ggatttccgc ggccacacca tcgaaccacg cgtcgaccat cactgaaggc
     9901 tgagagctga cagctacttc ttcttcggct
//
LOCUS       DATIFQ010000486         2667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1504260, whole genome shotgun sequence.
ACCESSION   DATIFQ010000486 DATIFQ010000000
VERSION     DATIFQ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504260"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1332)
                     /gene="rnd"
                     /locus_tag="VLE22_24590"
     CDS             complement(139..1332)
                     /gene="rnd"
                     /locus_tag="VLE22_24590"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HSB17649.1"
                     /translation="MATQSNYITSPAALGEFCKQLKDSSRLALDTEFVGEDSFVPKLE
                     LIQVATETTAAVIDFPAVLAAGSLDAFWEIVCNPAIQKVVHAGRQDLDLFAVHAGQIP
                     KPFFDTQIAAAMVGFGPQVAYANLVHRVHGTKLDKAHTFTNWSARPLSQDQLAYALED
                     VTFLLAIHDHLHGRLSKLGRLHWVDEEFSRLEGAVGETRREPQERYQRVRGWDTLKPK
                     SAAVLRELAAWREGEAKRRNVPRGRVMRDEVLLQLARHPPRHMQELRAVRGLHGSEAD
                     RNGEAILAAIQAALSLPASAWPEVPKERKPEAESAGLVELLQAVMKARATEEEIAPTL
                     LATTADLQALVDAKTNRAALDLPLLKGWRRVLLGDMLLDILDGKLAISIDPKSGTITW
                     NPPSP"
     gene            complement(1345..1977)
                     /locus_tag="VLE22_24595"
     CDS             complement(1345..1977)
                     /locus_tag="VLE22_24595"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HSB17650.1"
                     /translation="MPFSTAKYIKFRSGMQKRIDSLRSKTEDSMDVAVDTMMEERVDS
                     YFRVEQGLEEIVKSLSQIGDELEQVRDLSGAMRLESRLEFVEDRWDDLDSEIRERPRR
                     RRKKISLADMLKAAGGGGGTLSDNPSGVNNAVDAYAIMGVEFGSSLADVTAAFRIKAK
                     QLHPDANNGDRSSEPELRRMLEAYQFLKEYLNLSNTEPMRPPDRPYSPAE"
     gene            complement(2116..>2667)
                     /locus_tag="VLE22_24600"
     CDS             complement(2116..>2667)
                     /locus_tag="VLE22_24600"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HSB17651.1"
                     /translation="IILHGKTEQLRACRDALTACFTRDAPKGTLRDALKSADVFIGLS
                     VGNILSAEDLEVMAPDRVVFAMANPDPEVPPDLAASHCRIFATGRSDYPNQINNALAF
                     PGIFRGALDVQASEINEAMKLAAAKAIAHVIPEGALSEDYIIPSLFDKKVVPRVARAV
                     ATAARDSGVARRRARSDDQSLPR"
BASE COUNT          496 a          751 c          853 g          567 t
ORIGIN      
        1 cggcatctcg agaatcggca gacaaaacaa cgctgtgttt taaaggagtt gcaggcacgc
       61 ctggaaagaa tccggtcagg aagtcggttg ctgtgcagca tggaaagaac cggttgtccg
      121 gttttggaca gggcagggct acggcgacgg aggattccag gttatcgttc cggacttcgg
      181 atcgatggag atggcgagtt ttccgtcaag tatatcgagc agcatatccc ccagtaatac
      241 ccggcgccat cccttcagaa gcggaaggtc aagcgctgcg cgattcgttt tggcatcgac
      301 gagagcttgg aggtccgccg ttgtggccag caacgtcggt gcgatctctt cttctgtcgc
      361 acgggccttc atcacagcct gaagaagttc cacgaggccg gcagattccg cttcaggctt
      421 ccgttctttc ggaacctcag gccaggccga cgccgggaga ctgagtgctg cttgaatggc
      481 ggcaagaatg gcctcaccgt tgcggtcggc ttctgaccca tggaggcccc tgacagcgcg
      541 cagctcctgc atatgacgcg gagggtgtcg cgcaagttgc agcagcactt cgtcgcgcat
      601 cacccgcccg cgtggtacgt tgcggcgttt cgcctcccct tcccgccagg ccgccagctc
      661 tcgaaggacg gcggcggatt tcggtttgag cgtatcccag cctcgcacgc gctggtagcg
      721 ttcctggggt tcacgacgtg tctctccgac agcgccctcc aggcgggaaa attcttcgtc
      781 aacccagtgg agccggccga gtttggaaag acggccgtgg agatggtcgt gaatcgccag
      841 gagaaaggtc acgtcttcga gcgcgtaggc gagttgatcc tgagacagcg gacgggcgct
      901 ccagttggta aaggtgtggg ccttatccag cttcgttccg tgcacacgat ggacgaggtt
      961 ggcataggcc acctgtggcc cgaacccgac catcgcggcg gcaatctgcg tatcgaagaa
     1021 tggtttagga atctgaccgg catgaaccgc gaaaagatca agatcctgcc gcccggcgtg
     1081 cacgactttt tgaatcgccg ggttgcacac aatttcccag aaggcatcca gggagcccgc
     1141 tgccaggact gcggggaaat cgatgaccgc tgcggtggtt tccgtggcga cctgaatgag
     1201 ttcgagtttg ggcacaaagc tgtcttcgcc cacgaattcc gtatccagtg cgaggcggga
     1261 gctgtccttt aattgcttgc agaattcgcc gagtgcggca gggctggtaa tgtagtttga
     1321 ttgtgtggcc atcgttcctg tgacctattc ggccgggctg taggggcggt caggcggacg
     1381 cataggttcg gtgttgctca agttcaggta ttctttcaga aactggtagg cttcgagcat
     1441 gcggcggagt tcaggctcgg aactgcgatc gccattattg gcgtcaggat gcagttgctt
     1501 ggccttaatc cggaaggcag cggtcacatc ggcgagggaa ctgccgaact ccacgcccat
     1561 gatggcatag gcgtccactg cattgttcac accgctcggg ttgtctgaga gcgtcccccc
     1621 tcccccgccg gcggctttca gcatatcggc gaggctgatt tttttccggc ggcgacgggg
     1681 acgttcacgg atctcgctgt cgagatcgtc ccagcggtct tcaacgaatt ccagccgtga
     1741 ctccagccgc atcgcgccgg agaggtcgcg gacctgctcc aattcatctc cgatctgcga
     1801 gagcgatttg acgatctcct cgagcccctg ttcgacccgg aaatagctat ccacccgctc
     1861 ttccatcata gtgtcgacag ccacatccat gctgtcttca gtcttggagc gcagggaatc
     1921 gatgcgtttt tgcatcccgc tgcgaaactt gatgtattta gccgtcgaaa aaggcatgga
     1981 tcgcatgcac tcctggcgcg acgcattgta gcacagtccg attcctggtc agaagggctc
     2041 cgggatcgac tcctcatgtc atcgctgcta atcagaggag ggccaggtcg ggggtccgat
     2101 ggtctgctgg atgaattacc gcggcaagga ctggtcgtcc gatcgggccc gccttcgcgc
     2161 cacgccgctg tcgcgggcgg cagttgcgac ggctcgggca acgcgcggga caactttctt
     2221 gtcgaagagg ctggggatga tgtagtcctc actcagggcg ccttccggaa tcacgtgcgc
     2281 gatggccttc gccgcggcca gcttcatcgc ttcgttgatt tcgctggcct gaacgtcgag
     2341 ggcgccgcga aagatgccgg gaaatgcgag tgcgttgttg atctggttgg gatagtcgga
     2401 tcggccggtg gcaaagatgc ggcagtgtga ggcggcgaga tcgggcggga cctcggggtc
     2461 cgggttggcc atggcgaaaa caacgcgatc gggggccatc acctcaagat cttccgcgct
     2521 gaggatgttg ccgaccgaga ggccgatgaa tacgtcagcg ctcttcaggg catcgcgcaa
     2581 ggtgcctttc ggcgcgtcgc gggtgaagca ggcggtgaga gcgtcccggc aggcacgcaa
     2641 ttgctccgtt tttccatgca ggatgat
//
LOCUS       DATIFQ010000487        13019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1889722, whole genome shotgun sequence.
ACCESSION   DATIFQ010000487 DATIFQ010000000
VERSION     DATIFQ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 13019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13019
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889722"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1350
                     /locus_tag="VLE22_24605"
     CDS             55..1350
                     /locus_tag="VLE22_24605"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HSB17652.1"
                     /translation="MLLGRCAHRLTLDGTVEPLEDPQRKAILDINASLAHESLRVLAA
                     AYRRLGDAYANLQPDAVERDLVFLGLFAMKDPLRPEAVGAVRLCREAGIKTVMITGDH
                     KETAVAIARDLGLSDGPGTAFSGTEINHLTDEQMTQFVERVTVYARVSAEHKLRIVQA
                     WKRRGAIVAMTGDGVNDAPAIKAADIGVAMGLAGTDVTKEASDMVVTDDNFASIAAAV
                     EEGRGVFDNIRKTVHFLLSCNVSEVLVMLFATLLGLPLPLLPIQILWMNLVTDGIPAL
                     ALAVDPKAPDLMKRPPRRPDGRLLDSGRLLAIGGEGLMLGVIALVAFSYSLYGLHQHV
                     EQARTVAFTVMVVVQLVHAFNCRSERFSLFQIGVGTNRALLGAFLLSIAVQVAVLTVP
                     AAAPVFKVVPLPIEDWALMGTMGVLPFLIMESIKWLRRR"
     gene            complement(1370..3886)
                     /gene="mgtA"
                     /locus_tag="VLE22_24610"
     CDS             complement(1370..3886)
                     /gene="mgtA"
                     /locus_tag="VLE22_24610"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HSB17653.1"
                     /translation="MNNSPYWAIATHVLLTHLQTEPDGLRAKEASQRQARYASMRLKP
                     HQDIRPLLALLSQFRSPIILILLFAATVSLFLADRTDALIILTIILISALLGFWQEYG
                     AAKAVAALLSLVRVKTEVWREGQLIEVPSEEIVPGDVINLSAGSSIPGDGVVLESKDL
                     FVDEATLTGETYPAEKSATILSPETSLNQRTNSLFMGTHVVSGNAKAVIVHVGKETEF
                     GRIAAHVMLRPQETEFERGVRRFGYLLLEVTLLLVFAIFAINVYLHRPVLDSFLFSMA
                     LAVGLTPQLLPAIISVNLSHGAKRMAQQKVIVKRLASIENFGSMNVLCSDKTGTLTEG
                     TMRLHAALDLEGLPSERVLFHASLNATYETGFINPLDEALRRRCIFDLSRYRKLDEVP
                     YDFLRKRLSILVATPSTHLLISKGAVEQILAVCTGAELLDGTTAPMRERSEKIRHQFQ
                     DLNRQGLRTLALAYRDMGTTSRIDKGHETEMTFLGLLVFADPVRPEMAETISSLRRLG
                     VSLKIITGDHRLVASHVSQLVGMNHQRLLTGEELRHMTDEALLHRVNRIDVFAEIEPN
                     QKDRIILALKRSGNVVGYIGDGINDAPALHAADVGISVESAVDVAKDAADIVLLEKNL
                     GVLAQGVREGRTTFANTLKYVFMATSANFGNMFSMAGASLFLPFLPLLPKQILLTNLL
                     TDIPEMTIATDRVDRELIDRPRRWDIAFIRKFMLTFGLVSSVFDYLTFGALLLILNAS
                     PEQFRTGWFVESVISASAIVLVIRTRRPFFASRPGPPLVLATLAVALVTLLLPLLPIA
                     APLGLSPMPPSFLVLLAAILAGYVLTAELVKRRFYANGRV"
     gene            complement(3904..4803)
                     /locus_tag="VLE22_24615"
     CDS             complement(3904..4803)
                     /locus_tag="VLE22_24615"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSB17654.1"
                     /translation="MTVIQQNREVAQKLREVALLLDEQGANRFRVQAYRTAADRLERL
                     EVPVSQIAEQQGTDGLQALPGIGVSLARSIHTLVVTGRLPMLDRLRGHLDPIALLTSV
                     PGVGSALAHRLHDDLHIETLEQLEMAAHDGRLADIEGVGPKKLQGIIDSLTARLGRLR
                     RPTQPVGSHPTGEPSIEELLDVDREYRESALAGKLQTIAPHRFNPKKEAWLPILHTQR
                     GPRHYTALFSNSALANQLKKTRDWVILYYDDDHGEHQCTVVTGRTAPFAGKRIVRGRE
                     ADCASYYHSHESGATVGDSDSQA"
     gene            5376..5648
                     /locus_tag="VLE22_24620"
     CDS             5376..5648
                     /locus_tag="VLE22_24620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17655.1"
                     /translation="MQGWRCLMLALFCSAGTFGLAQESGAGIMTIQGVLTIIKGDVYT
                     MRDLFGRFVQFRVDKNTRRERLMVPGERVEVQMTPDHRAIAIKPIQ"
     gene            5711..6856
                     /locus_tag="VLE22_24625"
     CDS             5711..6856
                     /locus_tag="VLE22_24625"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB17656.1"
                     /translation="MRIAQVAPLWESVPPKLYGGTERIVSYITEALVCMGHDVTLFAS
                     GDSVTAARLDAICPQALRLNTGIFNRDAPMLMLQERALGMTGEFDVVHSHLDFLGFPL
                     ARRNPQPVITTLHGRLDLPELEPVFREYAEMPLVSISDAQRRPLPWANWQATVHHGLP
                     QDLYTYHPQHEGYLAFLGRISPEKRPDHAIELAKRTGLPLRIAAKVDPADTQYFKQQI
                     EPLLDHPLIEFIGEISDAQKNDFVGNALALVCPYDWPEPFGLVLIEALACGTPVLAYR
                     RGSIPEVIEDGVTGLVSENLSEMAESVAKIADIDRRRCRATFDERFTADRMARDYVAL
                     YERIIEERSAPKVHVVGSGAPIEGAAGVGSNGRRHERSLHVVGGRNV"
     gene            6849..8183
                     /locus_tag="VLE22_24630"
     CDS             6849..8183
                     /locus_tag="VLE22_24630"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB17657.1"
                     /translation="MSDGRASQEDSGQNRSRVLELLKTRDFGLLFAGQTISQIGDSLN
                     KVALLWFVYELTGSALKMTMVGLLQTLPPLLIGPLIGVYLDRFRKKPVMITVDLLRTV
                     MILLIPLLYLMDALTLDRLYVLVFANAIVSTIFGPALASAVPLITPKDRLVAANALMQ
                     STANVGLLVGPAISGIGIALIGAQNVLYVDAATFLISALCLMPIRMHETIDQTVLASK
                     GWTGGVAQDLLAGFRFVFVEQKTVLLLMLTATLYSVGVSAFVFLLPVFAKDVLAVGPV
                     QLGWLWSALGVGMLLASLGLASITQGDLSRRLQLISGALAIGGLAVGALGFLQTPLAA
                     GAMIAVIGGSTAMFTPLVWTMLQELTPAPLLGRVFTSFATGGMASSMAGMAGFGWAAD
                     TLGPAVSLGGISLILLLTAAAAGVFGRYKSQGAGLATNKPGAFAPQAVAAAP"
     gene            8575..9096
                     /locus_tag="VLE22_24635"
     CDS             8575..9096
                     /locus_tag="VLE22_24635"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSB17658.1"
                     /translation="MEREVKEKEKEKEKEKEKEAHKKPVGSLILTVKLALLADARLCH
                     FEIEAGENEQAITLEGRVAGESEKTAATEVARSVPGVKTVVNKLEVDKGLAQALVKKQ
                     DEIITGLIRERFSKSATLKSANFEVKTEEGVVSIIGVVRFQVIALEAAEAARRVPGVK
                     AVKTDKVRLEGES"
     gene            9096..10385
                     /locus_tag="VLE22_24640"
     CDS             9096..10385
                     /locus_tag="VLE22_24640"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB17659.1"
                     /translation="MQGRAGPSSCSPVVACETDHPATEPHPLLLTRNFGLVWSGQLIS
                     QIGDGISKLALLWFVYAITGSPLKTSVIGLLQTIPPIVLGPFIGVYVDRLPKKLLLIV
                     SDLLRAVLIGLIPCLIPVESFTVSVLYLLVLLHAIATAVFGPALTAAVPAIVPKTQFT
                     AANALLQGTTSLGIIFGPALSGLGIAAYGSQEVLCLNAVTYVVSAGCLIPVRFAGLVS
                     TGSPAGPPPAMLRELAEGLRYSILSRPVILLLTGTAALYTFGTSALTALFPVFARKML
                     GLGPVEVGYLWSALGIGLLVTSIGLLWVTEWTLKDRTRLIVGTSALSGAATGALIWTP
                     DPYHAGVLMGLIGCGMGAFTPIAWGVIQELSPRHMIGRILGLYGTGAMIAAIAGISAF
                     GWITERQGPETGLLGIGLVLLLTALVAWRMSRRGAEN"
     gene            10398..12566
                     /locus_tag="VLE22_24645"
     CDS             10398..12566
                     /locus_tag="VLE22_24645"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HSB17660.1"
                     /translation="MGDVVSWNNEFYIRASSSLADDRVEVLKQGETFAVFDRYGDIHP
                     ILPGPQGLYHEGTRFLSRCELLIGTQRPILLSATVKEDNALFTVDLTNPDMVLDGPRD
                     LRRGTFHLCRTRFLWQGVCYERLRVWNYGGAEIPLSLALAFDADYADLFEARGRRRAL
                     RGRRLDTVRSDKGLVLGYEGLDHVTRQTLIRCVPSPAHVTSDGVVLESSVAPQAGVTW
                     EIAVKCEIDQGRSRVFPSYDEARDDADCELKAAKARDCHIYTSNEQFNDWLNRSLADL
                     HMLISETPEGPYPYAGVPWFSAPFGRDGIITALELLWVNPVVARGVLGFLAATQATED
                     RPEQDAEAGKILHETRRGEMAALGEIPFGRYYGSVDVTPLFIILAGAYYEQSGDRAFM
                     QELWPSIRRALHWIDHRGDPKGTGFVTYARQSSDGLVHQGWKDSHDAVFHMDGTAAAG
                     PIALCEVQGYVYAAKRTAAELARLVGEVGRAEELLGEADSLRERFERAFWCEELDSYA
                     LALDGHGRPCRVRSSNAGQCLFSGIAGPERGLRTARTLLTEPYFSGWGVRTIATSEAR
                     YNPMSYHNGSVWPHDNALIAEGFARYGMNDLAARLLTGLFEASLFLDLHRMPELVCGF
                     ARRPGEGPTAYPVACAPQAWSAGAVFLLLKACLGLEIDAEARRLVLRRACLPAFLDTL
                     EIRNLRVAGEELDLLLERHAQNVGIQVLKRTGPVEIVSVK"
     gene            complement(12559..>13019)
                     /locus_tag="VLE22_24650"
     CDS             complement(12559..>13019)
                     /locus_tag="VLE22_24650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17661.1"
                     /translation="ELRGIELPGDPQLGRTFYATGLISGGVAPNVISPSAEAELNFRT
                     VGPGAEVLERLAPLRSRVAIETVLEVPPVRMRTVPGFESSAFAFTTDVPFLEGWGEPL
                     LFGPGSVVDAHTDGDHVATAELEAAVLAYERIARALLHEALGSSAPGRLT"
BASE COUNT         2384 a         3898 c         4186 g         2551 t
ORIGIN      
        1 gtcacccgtc ggacggctga gggccccatg gcctatgtca aaggggcgcc ggatgtgctg
       61 ctcggccgct gcgcgcatcg cctcacgctc gacggcacgg tggaaccgtt ggaggacccg
      121 cagcggaagg caattcttga catcaacgca tcactcgcgc acgagtcact ccgcgtgttg
      181 gctgcggcgt atcgtcgtct cggcgacgcc tatgcgaatc ttcagcccga tgcggtggaa
      241 cgggatttgg tgtttctcgg cctctttgcg atgaaggatc ccttgcgacc cgaagcggtc
      301 ggggccgttc gcctgtgccg ggaagccgga attaagacgg tcatgatcac cggtgaccat
      361 aaagagacgg cggtcgcgat cgcgcgggat ctgggcctct ccgacggccc tggaacggcg
      421 ttttcaggaa ccgagattaa ccatctcact gacgaacaga tgacccaatt tgtcgagcgg
      481 gtcacggtct acgcccgtgt ctcagccgag cacaagctgc gcatcgtcca ggcctggaaa
      541 cgcagaggtg cgatcgtcgc gatgacgggt gatggggtca acgacgcgcc ggccatcaag
      601 gcggcggaca tcggcgtggc gatggggctt gccggtactg atgtgacgaa ggaagcatcg
      661 gatatggtgg tgacagacga taatttcgcc tcgatcgctg cggcggtgga agagggccgg
      721 ggcgtcttcg acaacatccg gaagaccgtg cattttctcc tgtcatgcaa tgtgagcgaa
      781 gtactcgtga tgctctttgc gacgttgctg gggttgcctc tcccgctcct gccgatccag
      841 attctctgga tgaatcttgt cacggacggc atcccggccc tggcgctggc ggtcgatccc
      901 aaggcgccgg atctgatgaa gcggccgcct cgcaggccgg atggtcgctt gttggacagc
      961 ggaaggttgc tcgcgatcgg cggcgagggg ttgatgctgg gcgtcatcgc cttggtcgcc
     1021 ttcagctaca gcctgtacgg cctgcatcaa cacgttgagc aggcgaggac ggtggcgttt
     1081 acagtgatgg tcgttgtgca gctggtgcat gcgttcaatt gtcggagtga gcggttttca
     1141 ctgttccaaa tcggcgtcgg gacgaatcgc gccctcttgg gggccttcct cctctcgatt
     1201 gccgtgcagg tggcggtgct cacggttcct gcagccgcgc cggtgtttaa ggtcgttcct
     1261 ttgccaatcg aagattgggc gctgatggga acgatgggtg tgctgccgtt tctgatcatg
     1321 gaatcgatca agtggctgcg ccgacgatga gggattccgt gccggtgatt cagactcgcc
     1381 cgttcgcgta aaaacgccgc ttcaccagtt cagcagtgag gacatacccc gcaaggattg
     1441 ccgccaacag gacaagaaat gacggaggca tggggctcaa tcccaatggc gcggcaatcg
     1501 ggaggagggg aagcagcaac gtcaccaagg ccactgccag ggtagccagg acaagaggcg
     1561 ggcctggccg gctcgcgaag aacggccgcc tggttcggat caccaacacg attgccgatg
     1621 cggaaatgac ggattccacg aaccagccgg ttcgaaattg ttccggcgag gcattgagta
     1681 tcaagagcag ggcgccaaac gtgagatagt caaaaaccga actcaccagt ccaaaggtca
     1741 gcataaattt ccggatgaag gcgatatccc agcgtcgagg ccgatcgatg agttcacggt
     1801 cgacgcggtc agtggcgatc gtcatctcgg gaatgtcggt taggaggttc gtcaataaaa
     1861 tttgcttcgg cagcagcggg agaaacggga ggaacagcga ggcaccggcc atgctgaaca
     1921 tgttgccgaa gttggcgctg gtcgccatga agacatattt cagagtgttc gcaaacgtcg
     1981 tgcgtccttc tcggactcct tgagccagca ctcccagatt cttttcaagc agcacaatgt
     2041 cggcggcgtc tttggccacg tcgacggcgc tctcgacgga gatgccgacg tccgccgcat
     2101 ggagtgcggg agcgtcattg atcccatcgc cgatataccc caccacgttt cctgaacgct
     2161 tcagtgcgag aatgatgcga tccttttgat tgggctctat ttccgcaaag acgtcgatgc
     2221 ggttgacgcg atggaggagg gcttcgtctg tcatgtggcg aagttcctct ccggtcaaga
     2281 gccgctggtg gttcattcca accagttggc tgacgtggga tgcgacgagt cggtgatcgc
     2341 ccgtgatgat cttcagcgag acgcccagcc gtcgcaggga tgagatggtt tccgccattt
     2401 caggcctgac cgggtcggcg aagaccaaga gcccgaggaa cgtcatctcg gtttcatgcc
     2461 ccttatcgat gcgagacgtc gtacccatgt cacgatacgc caaagccagg gtccgaagac
     2521 cctgtcggtt caggtcctga aactgatgtc ggatcttctc gctgcgttcc ctcatggggg
     2581 ccgtggttcc gtccagcagt tctgcgccgg tgcagacggc caagatttgc tcgaccgcgc
     2641 ctttgctgat caggaggtgg gtgctgggtg tggccacgag aatggagagc cgtttacgca
     2701 ggaagtcgta cggcacctcg tccagtttcc ggtatctcga cagatcgaag atgcaccgcc
     2761 tgcgaagcgc ctcatcgagc ggattgatga aaccagtctc gtatgtggcg ttgagagagg
     2821 catggaagag gacgcgttca ctggggagac cttccagatc gagtgccgca tgcagccgca
     2881 tcgtgccttc ggtgagcgtg ccggtcttgt ccgaacagag cacgttcata ctcccgaagt
     2941 tttctatgga ggcaaggcgt ttgacgatca ccttctgctg ggccatgcgt ttagccccgt
     3001 gcgagaggtt gacgctgatg atggccggca gaagctgcgg ggtgagtccc acggcgagag
     3061 ccatggaaaa gagaaacgag tcgaggaccg gccggtggag atagacgttg atggcgaaga
     3121 tggcaaatac gaggagcaac gtcacttcga gcaacaaata tccaaagcgc cggacgccgc
     3181 gttcgaactc cgtttcctga ggccgaagca tcacgtgagc agctatgcgg ccgaattccg
     3241 tctccttgcc cacatgcacg atgacggcct tcgcgttccc gcttaccacg tgggtcccca
     3301 tgaacaggct attcgtccgc tgattcaggg aggtctcagg ggacaagata gtcgccgatt
     3361 tctcggccgg ataggtttcg ccagtcaagg ttgcttcgtc gacgaagaga tccttggatt
     3421 cgagcacgac cccatcgccg gggatgcttg agccggcgga gagattgatc acatctccgg
     3481 gaacaatttc ttcgcttggc acttcaataa gttgcccctc acgccagact tcggtcttga
     3541 cccgaacgag tgagagtaat gcggcgaccg ctttcgccgc gccgtactcc tgccaaaacc
     3601 ccagcaacgc actgataaga ataatggtca ggatgatcag cgcatcggtg cgatctgcaa
     3661 ggaacagcga taccgtggct gcaaacagca gaatgaggat aatcggactg cggaattgcg
     3721 agagaagggc aagtaaaggg cgaatatctt ggtgtggctt gagccgcatg gacgcatagc
     3781 gcgcttgacg ttgggaggcc tccttagcac gaagaccgtc cggttccgtc tgcaggtgag
     3841 tgaggagtac atgcgtcgct atcgcccaat atggactgtt attcacagga acacctctct
     3901 cgattacgcc tgactatctg aatccccgac agtcgcacct gattcatggg agtgatagta
     3961 cgatgcacag tcggcttctc ttcctcgaac gatgcgcttc cctgcgaagg gcgcagtccg
     4021 tccagtcacc accgtgcact ggtgttcgcc gtggtcgtcg tcgtagtaga ggatgaccca
     4081 gtcccgggtc ttctttaact ggttggccag ggcgctgttg gagaacaggg cggtgtagtg
     4141 gcgggggccg cgctgggtgt gaaggatcgg cagccaggcc tctttcttcg gattgaatcg
     4201 atgcggcgcg atggtttgta actttcccgc tagagcagac tcccggtact cccggtccac
     4261 atcgagcaat tcctcgattg aaggttctcc tgtcgggtga cttccgaccg gttgtgtcgg
     4321 cctgcgaagg cggcccagtc tggctgtgag ggaatcgatg ataccttgta gtttcttggg
     4381 accgacccct tcgatgtctg ccagccgtcc atcgtgcgcc gccatttcca gttgttccaa
     4441 cgtttcgata tgcagatcgt cgtgcagccg gtgggccagg gctgatccga cgccggggac
     4501 cgacgtcagc aaggcgatcg gatcgaggtg cccgcgcagg cgatccagca tcggcagccg
     4561 gccggtgacg acgagcgtgt ggatcgagcg cgccaggctc acgccgatgc cgggcaaagc
     4621 ttggagcccg tccgtgccct gttgttcggc aatctgcgag acagggactt ccagccgctc
     4681 caaccggtct gcggcggtcc gataggcctg gacccggaaa cggttcgcgc cctgttcgtc
     4741 caggaggagc gcgacctccc gcaacttctg ggcaacctcg cgattctgct gaatgacggt
     4801 catgggcggc gacgggctac tcctaaaaca aaacgacttg gctggaagct ttatcagcca
     4861 ggtgaaagtc ggaggtcagg ccgatgacga aggaaaagaa tcaggcagcg gctgcggttc
     4921 ctgacccaga gtttcatctg cctccgccac tcagtcgttc gtcggtgcag tcaaacctgc
     4981 ttccggccat atcagacgga tctgagtcgg gattgggttc cgaccattag cactcatggg
     5041 agcccgggaa gcagtctccc aaccccccct ctgccactct attccatcgg cgcacgcgtt
     5101 cccaaggcct ccccgagcac attagatcgc ctgccacatc gaaggtgccg tgcgtgcttc
     5161 tgatcgacct gcccgtgact agccgggggc atcatagagc cgaggcatgt caagggaggt
     5221 aattggacat atttaatgct gaggcaaggc atttgtccat tgtgggggga ggggggcagt
     5281 tcagtagcat atgcctatga aagttcgggt tcggggagta cagcacgagc gagttgcggc
     5341 agggctgctt gccgcatgcg atcgaggggt gccccatgca gggatggcga tgcctcatgc
     5401 tggcgctgtt ctgttcagcg gggacgttcg gactggcaca ggaatccggc gccgggatca
     5461 tgaccattca gggcgtgctg acgatcatca agggcgacgt ctacacgatg cgggacctgt
     5521 tcggccgctt cgtccagttc cgggtcgata agaacacacg gcgggaacgg ctgatggttc
     5581 ctggcgaacg tgtcgaggtg cagatgacgc ccgatcatcg ggccatcgcg atcaagccga
     5641 ttcaatgatc gagtccggcg cgcgaatgag tcgggcaccg gtagcattag caggctgaaa
     5701 ggaccaatct atgagaatcg cgcaagtggc tcctctatgg gaaagcgtgc ctcccaagct
     5761 ctacgggggg actgaacgga ttgtttcgta catcacggaa gcgctggtct gcatggggca
     5821 cgacgtgaca ctctttgcca gcggggattc tgtgaccgcg gcgcgactgg acgccatctg
     5881 ccctcaagcc ctccggctca acaccggcat cttcaatcgt gacgccccga tgctcatgct
     5941 gcaggagcgg gcgttgggga tgacgggcga gttcgacgtg gtgcactcgc atctggactt
     6001 tctcggattt cccctggccc ggagaaaccc gcagccggtc attacgaccc tccacggacg
     6061 tctggatctg cccgagttgg agccggtctt ccgcgaatac gccgagatgc cgctggtctc
     6121 gatttcggat gcgcagcgcc ggccgctgcc ctgggcgaac tggcaggcga ccgtgcacca
     6181 cggactcccg caggacctct acacgtatca tccacagcac gaaggctatc tagccttcct
     6241 gggacggatc tctccggaaa agcggccgga ccatgcgatc gaactggcga agcggaccgg
     6301 gctcccgctc cgcatcgcgg cgaaggtgga cccggcagat acccagtatt tcaagcagca
     6361 gatcgagcct ttgctcgacc atccgctgat cgagttcatc ggcgaaatct cggacgcgca
     6421 aaagaacgac tttgtcggga acgccctggc gttggtttgc ccgtacgact ggccggagcc
     6481 ttttgggctg gttctcattg aagccctggc ctgcgggacg cccgtcctgg cctaccggcg
     6541 cgggtccatt cctgaagtga tcgaggacgg tgtcaccggc ctggtttccg aaaatctgtc
     6601 ggagatggcg gagtcggtcg caaagattgc cgatatagac cggcgccgct gccgggcgac
     6661 atttgatgag cggttcaccg cagatcgaat ggcgcgagac tacgtcgccc tatacgaacg
     6721 gatcatcgaa gagcggagcg ctccgaaggt gcatgtcgtc ggatccggcg cgccgatcga
     6781 gggggcagcc ggcgttggtt ccaacgggcg acgccacgaa cggtcgctgc acgttgtggg
     6841 aggacgaaat gtctgatggt cgcgcctcac aggaggattc cggtcagaac cgttcccgcg
     6901 tgttggaatt gctcaaaacg agggacttcg gactcctctt cgcaggccag acgatctcgc
     6961 agatcggcga cagcctgaac aaggtggcgc tgctctggtt cgtgtatgaa ttgaccggat
     7021 cggctctcaa aatgacgatg gtggggctgc tccagacgct cccgccgctc ctgatcggac
     7081 cgctgatcgg ggtctacctg gaccgcttcc gcaagaagcc ggtcatgatc accgtcgatc
     7141 tgctccggac cgtgatgatt ctgctcatcc cgttgctcta tttgatggac gccctgacgc
     7201 tggaccggct ctacgtgctg gtctttgcca acgccattgt ctcgaccatc ttcggcccgg
     7261 ctctggcctc cgcggttccg ttgattacgc ccaaggaccg gctcgtggcc gccaacgcgt
     7321 tgatgcaatc caccgcgaac gtcggactcc tcgtcggtcc tgccatcagc gggatcggca
     7381 tcgccctgat cggagcccag aacgtgctct acgtggacgc tgccacgttc ttgatctcgg
     7441 ccctctgcct catgccgatc cggatgcacg aaacgatcga ccagacggtg ctcgcgagca
     7501 aggggtggac tggcggtgtc gcgcaggatc tgctcgcggg attccggttt gtgttcgtcg
     7561 aacagaagac cgtcctcctg ctgatgctga ccgctaccct gtacagcgtg ggcgtcagtg
     7621 ccttcgtctt tctgctgccg gtgttcgcga aagacgtgct cgcggtgggg ccggtgcaat
     7681 taggctggct ctggtctgcg ctcggtgtcg gtatgctgct tgcttcactc ggtcttgcgt
     7741 cgatcaccca gggagatctc tcacggcgtc tgcaactcat ctccggcgcc ctggcgatcg
     7801 gcggactcgc cgtcggcgca ctagggttcc tacagacgcc gctggcagcc ggagctatga
     7861 tcgccgtgat cggaggcagc acggcgatgt tcactcccct ggtctggacg atgctgcagg
     7921 aattgacgcc ggctcctctg ctgggccgtg tctttactag ctttgccacg ggcgggatgg
     7981 cctcgtcgat ggccgggatg gcgggattcg gatgggctgc cgacacgctg ggtcccgctg
     8041 tcagcctggg cggtatcagc ttgatcctcc tgctgactgc ggccgcggct ggagtgttcg
     8101 gccggtacaa gtcgcagggg gccggtctcg cgacaaacaa accgggagca ttcgcccctc
     8161 aagcggttgc ggccgccccg tgaccactga ccggcacgct ctctactcct tgaggtggag
     8221 tcttcctagg agccataata aacggcgcac cgcggcaatc gtgagtccta ccgcatgatg
     8281 ccatgttgtc gaatcctgtt ctttgcggcg atcagcagct ttttcctgtc gccccttcct
     8341 tcagataaag cgttctcgga acagcaggac aaagagaagg cgcaggacaa accgcaggag
     8401 atgccaccgg cgcagcctca gcctcatcct gatactcaac ctgctccacc caagctcttg
     8461 accgagccca agccgcagag cacgccatcg gcgaagcccg aggcaccccc gaaactttca
     8521 actgagccta aaacccagcc ccagcctgcg cctcctgcca agccggagcc gtccatggag
     8581 cgggaggtaa aagaaaaaga gaaggagaaa gaaaaggaaa aagagaaaga ggcgcacaag
     8641 aaacccgtcg gctcgctgat ccttaccgtc aagctcgcgc tgctggccga tgcccggctg
     8701 tgccactttg agatcgaggc cggggagaat gagcaagcca tcacgctcga ggggcgagtc
     8761 gccggcgaga gcgaaaagac ggccgcgacc gaggtcgcca ggtccgtgcc cggcgtcaag
     8821 accgtcgtca acaagctcga ggtagataaa ggcctcgcgc aggcgctggt caagaagcag
     8881 gacgagatca tcactggtct catccgggaa cggttttcca agagcgccac gctcaagtcc
     8941 gcgaatttcg aggtcaagac cgaagagggc gtcgtctcga tcattggagt cgtgcgattc
     9001 caggttatcg cccttgaagc ggcggaagcg gcccgccggg tgccgggggt caaggccgtc
     9061 aagaccgaca aagtgcgact ggagggcgaa agctaatgca gggccgcgcc ggcccctcgt
     9121 catgttcccc cgttgtggca tgcgagacgg accaccccgc aaccgagcct catcccttgc
     9181 tgctcacccg caatttcgga ctggtctggt ccggacagct catctcgcag atcggcgacg
     9241 ggatttctaa gctggccctg ttgtggtttg tctacgcgat taccggctcg ccgctgaaaa
     9301 cctccgtgat cgggctgtta cagacgatcc cgccgatcgt ccttggtccc tttatcggcg
     9361 tctacgtgga ccggctgccg aagaagctcc tgctcatcgt cagtgatctg cttcgcgccg
     9421 tcttgatcgg gttgattccc tgtctcatcc cggtcgaatc gttcaccgtg tccgtgctct
     9481 atctcctggt gctcctgcac gccattgcga ccgccgtctt cggcccggcg ctcaccgctg
     9541 cagttccggc cattgtgccc aagacgcaat tcaccgctgc caacgcgctt ctgcagggca
     9601 caacgagtct cggcattatt ttcggtccgg cattgagcgg gttgggcatc gcggcgtacg
     9661 gatcgcagga agtcctgtgt ctcaacgccg tcacttacgt ggtctcggcc ggatgtctca
     9721 ttccggtccg gtttgccggt ctcgtttcga cagggagccc tgcggggccg ccgccggcga
     9781 tgctgcgaga attggcagaa ggactgcgct acagcatcct cagtcgtccc gtgattctgc
     9841 tcttgaccgg caccgcggcg ctatatactt tcggcaccag cgcgctgacg gcgctcttcc
     9901 ccgtgttcgc ccggaaaatg ctcggcctgg gtccggtcga ggtcggctac ctgtggtccg
     9961 cgttgggcat cgggttgttg gtgacgtcga tcggcctctt atgggtcacc gagtggacgc
    10021 tcaaggatcg gacccgtttg atcgtgggca ccagcgcgtt gagcggcgcc gcgaccggcg
    10081 cgttgatctg gactcccgat ccctaccacg ccggcgtgct gatgggattg atcgggtgcg
    10141 gcatgggagc cttcacgccg atcgcctggg gggtcatcca ggaactttcc ccgcgccata
    10201 tgatcggccg gatcctgggt ctgtacggaa ccggcgccat gattgccgcc atcgccggga
    10261 tttccgcctt tggctggatc acggaacggc agggaccgga gaccggtctg ctgggaatcg
    10321 gcctggtgct gcttctcact gctctcgtgg cctggcgaat gagccgcagg ggcgcggaga
    10381 attgagagga gtggtcaatg ggcgacgttg tcagctggaa caatgaattc tacattcgtg
    10441 cttcctcctc gctggcggac gatcgtgttg aagtgttgaa gcagggcgag acgttcgccg
    10501 tcttcgatcg ctacggcgat atccacccga tcctgccagg tccgcaaggc ctctatcatg
    10561 aaggaacacg attcctctct cgttgcgaac tcttgatcgg gacccaacga ccgatactcc
    10621 tgagcgcgac ggtgaaggag gacaatgcgc tattcacggt cgatctgact aatccggata
    10681 tggtgctgga cgggccgcga gacctgcgcc gcggcacgtt ccacctgtgc cggacacggt
    10741 ttctgtggca aggcgtctgc tatgagcgtc tccgcgtgtg gaattacggc ggggcggaga
    10801 ttcctctttc cctcgcgctt gcgttcgatg ccgattatgc ggatctgttc gaggcgagag
    10861 gccggcggcg ggcactgcgg gggcgccggc tcgacacggt ccggtccgac aaggggctgg
    10921 tgctcggcta cgaaggattg gatcacgtga cccgtcaaac tcttatccgg tgcgttccgt
    10981 cgccggcgca tgtgacatcc gacggggttg tcctcgaatc gtccgtggcc ccgcaggccg
    11041 gcgtgacctg ggagatcgcc gtcaaatgtg aaatcgacca ggggcgctcg cgcgtcttcc
    11101 cgtcctacga tgaagccagg gatgacgcgg actgtgaact caaggcggcc aaggcccgcg
    11161 attgccatat ctacacctcg aacgagcagt tcaacgactg gctgaatcgg tcgctcgccg
    11221 atctccacat gctgatttcc gagacgccgg agggacccta tccctatgcc ggcgttccct
    11281 ggttcagcgc ccctttcggc cgcgacggca tcatcaccgc gctggagctg ttgtgggtca
    11341 atccggttgt cgcacgcggc gtgctggggt ttctggctgc gacacaagcg acggaggatc
    11401 gtccggaaca ggatgcggaa gcggggaaga ttctgcacga gacccgccgg ggcgagatgg
    11461 ccgcgctggg agaaattccg ttcggccgct attacggcag cgtcgacgtg accccgctgt
    11521 ttatcatcct ggcgggcgcc tattacgagc agagcggcga tcgagccttc atgcaggaac
    11581 tctggccgag catccggcgc gccttgcatt ggatcgatca ccgcggcgat ccgaagggga
    11641 cgggctttgt cacctacgcg cgccagtctt ccgacgggct cgtgcatcaa ggatggaagg
    11701 actctcacga cgccgtgttc cacatggacg gcacagccgc cgccggtccc atcgcgctct
    11761 gcgaggttca gggctacgtg tatgccgcca aacggacggc ggcagagctg gcgcgacttg
    11821 tcggggaagt cggtcgcgcg gaggaattgc tcggtgaagc cgactcgctg cgcgaacggt
    11881 tcgaacgcgc gttctggtgc gaggaactcg actcctatgc gctggcgttg gacgggcacg
    11941 gccgaccctg ccgcgtccgt tcgtccaacg cgggccagtg cctgttctcc gggatcgccg
    12001 gaccggaacg gggactgaga acggcgcgga cactattgac cgagccctat ttcagcggat
    12061 ggggcgtccg gacgatcgcg acatccgagg cgcggtacaa tcccatgtcc tatcacaacg
    12121 gctcggtctg gccgcacgac aacgccctga tcgcggaggg attcgcccgc tacggcatga
    12181 acgacctcgc ggcgcggctc ctcacggggc tcttcgaggc gagcctcttc ctggacctgc
    12241 accgcatgcc cgagctcgtc tgcgggttcg cccggcgccc gggggaggga ccgaccgcgt
    12301 acccggtcgc ctgcgccccc caggcctggt cggccggcgc cgtcttcctg ctcctgaagg
    12361 cctgcctcgg gctggagatc gacgccgagg cgaggaggct ggtcctgcgg cgcgcctgcc
    12421 tgcccgcgtt cctcgacacg ctggagatca ggaacctccg ggtggcgggc gaggagctcg
    12481 acctgctcct ggagcgccac gcgcagaacg tgggcatcca ggtcctgaag cgcaccgggc
    12541 ccgtcgagat cgtctcggtc aagtgaggcg gcccggggcg ctcgagccca gggcctcgtg
    12601 gagcagggcg cgggcgatcc gctcgtaggc gaggaccgcc gcttccagct ccgcggtcgc
    12661 gacgtggtcg ccgtccgtgt gcgcgtccac gaccgagccg gggccgaaga ggagcggctc
    12721 gccccagccc tcgaggaacg gcacgtcggt ggtgaaggcg aacgccgagc tctcgaaccc
    12781 cggcacggtg cgcatgcgca cgggtggaac ctcgaggacg gtctcgatcg cgacccggga
    12841 gcgcaggggg gcgagccgct cgaggacctc ggcgccaggc ccgaccgtcc ggaagttgag
    12901 ctcggcctcc gccgagggcg agatcacgtt gggagcaacg ccgccggaga tgagcccggt
    12961 cgcgtagaag gtgcggccca gctgcgggtc cccgggcagc tcgatcccgc gcagctcga
//
LOCUS       DATIFQ010000488         2471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1123335, whole genome shotgun sequence.
ACCESSION   DATIFQ010000488 DATIFQ010000000
VERSION     DATIFQ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123335"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1225
                     /locus_tag="VLE22_24655"
     CDS             206..1225
                     /locus_tag="VLE22_24655"
                     /inference="COORDINATES: protein motif:HMM:NF018123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HSB17662.1"
                     /translation="MEGNFGIASLLILISGVLNASFPLPMKFSRDWRFENNWLAFTTL
                     ALLVVPAGLAMMIVPEFRQIYAAASPGEFLPGIVFGFLWGTAQVTFGLSIEMVGMAMA
                     FAIVVGMSAVIGSLTTLLVLNPEEALGSRGLILMASALILAAGLTLYAKAGNRRETES
                     GSAARSGASFRKGMAVCLYTGAMGGMINLGFAFSDGILGRAKLAGASPLVATLAVWPV
                     VLAAGYVPNLAYTAYLLVRNRSGAVFGRSTGRGVLLAAAAAFFWLGGTLGYGLGAATM
                     GRYGTSIGFALYMTILLLWSTILGILTHEWRSASRGTLRLMQAGVVVILLSVLVLSAG
                     GLLGS"
     gene            complement(1228..>2471)
                     /locus_tag="VLE22_24660"
     CDS             complement(1228..>2471)
                     /locus_tag="VLE22_24660"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSB17663.1"
                     /translation="HAGRVDLNETLKEGGRGGTSGARSHRARGALVIAEVALAAMALI
                     GAGLSVRTFQKLARVNLGFEPRNVLVAHFHLSTNGYSLSQEKQFNRNLRLRLEAARGI
                     EHVSYGNAVPVSIFAGGTDRVQAFGTEADEQGVITLQRNVVAAGYFRLMGIPLLEGRD
                     FTEQDDLGTAPVIVVNETFARRYFDGKYPIGRKVRVAGNWSTVVGMVKDSKYRTPAEG
                     ATAYFYGSFGQMFWSGHDNFVYIRAKDLDEARGTLRREVAALDPNKGLYELSTLEEYT
                     QAGLFGERVAASLLSALGILALALAAVGLYSVMAYAVSERTQEIGIRMALGAPRHQVL
                     GLMLRRGLVMTITGLAAGIVSAIAAARVLSSAIETPISADEPAVFALATLTLVLTALL
                     ASYIPARRATKVDPLTALRSE"
BASE COUNT          430 a          779 c          780 g          482 t
ORIGIN      
        1 cgagacctat caatccagcg aagacaacgc gttttggacg agaggttacc ctgacgagtt
       61 gaaactgcgg ctctccgggg agctccagaa gcgataccac aagctgaaac agggcgaaat
      121 gttcgtggtt cgatgagcgt ccattgcggc gagttgccgg cctgatccgg ggacgagaat
      181 tgaattacaa tcgggtcctc atatcatgga aggcaatttc ggcatcgcat ccctgctgat
      241 tctgatcagc ggcgtcctga acgcctcctt tccactcccg atgaaattct ctcgggattg
      301 gcggtttgaa aataactggc tggcgttcac caccctggcc ctgctggtgg ttccagcggg
      361 actggcaatg atgatcgtgc cggaatttcg gcagatttac gcggctgcgt cgcccggtga
      421 gtttctgccg ggcatcgtat tcggctttct ttggggcacc gcgcaggtga cgttcggcct
      481 gagcatcgag atggtcggca tggcgatggc gttcgccatt gtggtcggga tgagcgctgt
      541 gatcggcagc ctcacgaccc tattggtgct caatccggag gaagcgttgg ggtctcgggg
      601 actgatcctg atggcgagtg cgctgatcct ggcggcgggg ttgacgctct atgcgaaggc
      661 gggcaaccgg cgggagaccg aatccgggtc ggcggcgcgc tcgggggcgt cgttccgcaa
      721 aggcatggcc gtctgccttt acaccggagc gatgggcggc atgatcaacc ttggattcgc
      781 ttttagtgac ggcatcctcg gaagggcgaa actggcgggc gcttcgccgc tcgtcgcgac
      841 actcgcggtc tggccggttg tgctggcggc gggctacgtg ccgaacctcg cgtacacggc
      901 gtatctcctc gtcaggaatc ggagtggcgc cgtattcggg cgatcaacgg ggcgcggcgt
      961 gctcctggcc gcggcggctg ccttcttctg gctgggcggg acccttggct acgggcttgg
     1021 cgcggcgacg atgggccgct acggcacgtc gatcggattt gccctataca tgaccatcct
     1081 tctgctgtgg tcgacaatcc tgggcatact gacacatgaa tggcgcagcg ccagccgcgg
     1141 cacgctccgt ctcatgcagg cgggagttgt ggtaatcctg ctgtcggtgc tggtgctcag
     1201 cgcgggcggg ctgctcggaa gttagcccta ttcagaccgg agggcggtga gggggtccac
     1261 tttggtagcg cggcgggccg ggatgtagct ggcgaggagc gctgtcagga ctaaggttag
     1321 cgtcgccagg gcgaacacgg ccggctcatc agcgctgatc ggcgtttcga tcgcgctcga
     1381 caggacacga gcagccgcga ttgcggaaac gatgcccgcc gcgaggcccg tgatcgtcat
     1441 caccagtcct ctgcgcagca tcagcccgag cacctgatgc cgaggtgcgc cgagcgccat
     1501 gcgaatgccg atctcctgcg ttcgctcgct caccgcatac gccatcacgc tatacagacc
     1561 gaccgccgcc agcgccagcg cgagaatgcc gagcgcggaa agcaggcttg ccgcaaccct
     1621 ctcgccgaat aggcccgcct gcgtgtactc ctccagcgtc gacagctcgt agagtccctt
     1681 gttcggatcg agagccgcca cttcgcgccg caatgtcccc cgcgcctcgt ccagatcctt
     1741 ggcccggata tatacgaagt tgtcgtgacc cgaccagaac atttgtccga acgatccgta
     1801 gaaataggct gtggcgcctt ccgccggggt acgatacttg ctgtccttca ccataccgac
     1861 caccgtcgac cagttacccg caacgcgcac cttccggccg atggggtact tcccgtcgaa
     1921 ataccgtcga gcgaacgtct cgttcacgac gatcaccggc gcggtgccga ggtcgtcttg
     1981 ctccgtgaag tcgcgaccct ccagaagcgg gatacccatc aggcggaagt agccggcagc
     2041 aaccacattc cgttgcagcg tgatgacccc ttgctcgtcg gcctccgttc caaacgcctg
     2101 aacccggtcg gtccccccgg cgaagatcga aaccggcacc gcgttgccgt agctcacgtg
     2161 ttcgattccc cgagcggcct ccagccgcag ccgcaaatta cgattgaact gtttttcctg
     2221 gctcagggag tacccgttcg ttgacaggtg gaaatgcgcc acaagcacgt tgcggggctc
     2281 gaagcccaga ttgacccttg cgagtttctg gaaggtccga accgacaggc ccgcccctat
     2341 gagcgccatt gccgcaagcg ccacctcggc aatcacaagc gctcctcgcg cccggtgcga
     2401 gcgtgctccg gacgtcccgc cgcgcccgcc ctccttcaac gtttcgttca agtccacgcg
     2461 gcccgcatgg a
//
LOCUS       DATIFQ010000489        17653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_549478, whole genome shotgun sequence.
ACCESSION   DATIFQ010000489 DATIFQ010000000
VERSION     DATIFQ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17653
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549478"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="VLE22_24665"
     CDS             <1..1767
                     /locus_tag="VLE22_24665"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HSB17664.1"
                     /translation="RVTRFRYQPPSTQPPSYNSPLMLPDLLHLHTYFLFPFSIDKQAV
                     LEDHRDLWAKYPRWIDGLDEWIAVHRTRASSTVAQHLGPWRRAAYSRFDLDSPAYQDM
                     VFFHPIVRRVFFDTTDAAGGEKEALLRCYTIPLGADGKVWLEAEDRKGRSARVQITEL
                     RLFLFANGIGILSIGVEAFHISAADALWINESLRKVYPSSGRQLREARVPDRLTIAIE
                     RGGVREIVAEERFEKCDMIGFLPPLSSTIASLLYFTDYEREEYEPVLDERMIVYSYCA
                     VDPAAISPEFILSEDYQVFLSRFLYVDRWGPGYRYEPVFTREEMRRQFYTRWAHQGTY
                     YGFTSYSNITSTIGTFDCDEHQLLEGFLVHRMFETRYYLMALVALFYRATLLDFSERT
                     ALVSKQLYLDQEDGRIGVENIGMANDLRADFLHFSNYWHFDELANKDEELDHFTLQCR
                     EYRIRPTKEEVEEEIEKLNASLHTYHQFRNTEAINRVAMLSLLFGAGAVLTGFFGMNF
                     GHRFGRLFFEPDQSTLPFHYAAVVAVLLLAFGALAFGIFVVIANWSDYKEIFMFRRGS
                     NRLPGDVGSLRRRPPPVSRPQN"
     gene            1987..4473
                     /locus_tag="VLE22_24670"
     CDS             1987..4473
                     /locus_tag="VLE22_24670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17665.1"
                     /translation="MENLLRDARFSTLILLKTPAFTAVAVLSLALGIGANTTIFSVVN
                     AILLRPLPFKQPDRLVTISERNREKNQGSRNPRLSTAIEWREKTRSFAQVELAVPYTE
                     STTLSGQAWAEQVIIQFLDRDCFSLLGSKPILGQTFPAGEVIDNTSRNILISHGFWQR
                     RFGGDQKVIGQTLIGGDGPWTIVGIMPPGYWIFPWAKDADVFSAVSLTKNKLTPETRW
                     FSVLARLKPDATLAQAQAEMDVFARRIEQQYPQTNKGWDVKVEPLQQYLFRGWGDVLY
                     LLLGTVGFVLLIACANVANLLLARAAMRQKEIAIRASLGAGRWRLIQQLLTESVVLAV
                     VSGLLGILVSLAGIRIFVALAPEWFPRAEEIGIDFRVLGFTLGVSLLTGILFGLAPAW
                     KASRPDLTESLKEGGRSSGGELRQYGRSLLVVSEVALAMVLLIGAGLMINSFLRLRFA
                     EPGYNPRNLLTAQIQLDGPRYRELLEGDMKRVTPRVDIFFEQVLERLQGQPGVEAAAV
                     ASRFPMQRAFRIIGRPEPPTNQQPVGAVLEVTAGYFSTMQVPLRRGRTLTEGDNQSAS
                     WVVVVNDTLAKRYFPDEDPIGKTIQLKFGDTSGVNLDENKPREIIGVVGSVKYWGLSN
                     DPPPALYVPYRQHMSDYPGGTSQTRLGKTLLLRTKSDPMHLAGAVRRVVAEADKDQPV
                     SEMTSMEQTLSEWLAPQRFAMQLFGIFAALAVILAVVGIYGVMSYSVSRRTHEIGLRL
                     ALGAERDDVLKLVLRRGLKLSLIGVAIGIAGALAMTRLVSGFLYGVKASDPLTFAAVS
                     LLLILVALAASYIPARRATTVDPMAALRYE"
     gene            complement(4536..5099)
                     /locus_tag="VLE22_24675"
     CDS             complement(4536..5099)
                     /locus_tag="VLE22_24675"
                     /inference="COORDINATES: protein motif:HMM:NF015629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HSB17666.1"
                     /translation="MRSCPTARATDSPEGLTEPPRGVAESYAVIKAVSVTVLLSDIVE
                     ALEMQFDESSSFLDLDTLQVVTVSYKLLREAEENAGGQEPNLHDWQEEEWQAAKRIVS
                     TDRFLGLPTKFDVHEWAIMRDFANSVKRDSIREDLLSAIHGGGAFRHFKHRVRAHNIE
                     SAWFAFRSEALRQIAVDWCEEHHIRWR"
     gene            complement(5161..5793)
                     /locus_tag="VLE22_24680"
     CDS             complement(5161..5793)
                     /locus_tag="VLE22_24680"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB17667.1"
                     /translation="MFARILLLFAGVTMAWAQGGKPVLPGEDWVSLFNGQDLTGWVKV
                     GNEKWLVENATIHGQGITKEYGYLRTEKKYKDFHMFIRFKCEAKGNSGVFFHTDFEPG
                     TADVSQGLQFEIDKTVGRHSGGIYGDGRAWIVWPAPELEPLIKPDDWNEMLMKVEGNR
                     YVSYLNGVRMIDFTDPTPKSFDGYIALQLHSGGEGDMRFKDIYIRDLTKR"
     gene            complement(5860..7221)
                     /gene="glmM"
                     /locus_tag="VLE22_24685"
     CDS             complement(5860..7221)
                     /gene="glmM"
                     /locus_tag="VLE22_24685"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSB17668.1"
                     /translation="MPKRLFGTDGIRGEAGTFPLDPLTVGASGEALGHLATRLRQDPE
                     VLVGIDTRESSQWIAAQLAGGLVRGGARPRFAGLITTPGVAYVTRTGPFVAGVMISAS
                     HNPYQDNGIKIFGHSGYKFPDAEEKAIEREIFALLEAGVRAEPAELREDPGLDASYVD
                     FLASTFPGTLEGMRVVADCANGAASHLAPELFGRLGARVDAIHCSPNGRNINLDSGAL
                     HVDGLRCAVVELGADIGVALDGDADRAIFASHSGRAVNGDGVLLITARHMLERGELPG
                     PDGEPTVVSTVMANLGLEKALHRLGIRMLRTAVGDKYVLEEMLRCGAMLGGEQSGHVI
                     FRQYATTGDGLLTALRVLEVMRETGKDLDVLTRDFEVHPQRLVNIRVREKAPLEELPE
                     VAAELRAASEAFGDAGRILVRYSGTELLARVMVEGVDEGRVELFAERIAESIRRAIGA
                     VGS"
     gene            complement(7221..7886)
                     /locus_tag="VLE22_24690"
     CDS             complement(7221..7886)
                     /locus_tag="VLE22_24690"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HSB17669.1"
                     /translation="MKRLLLENIGWKILSLALAVALWLAVVGEPQLATSISAPLEFRN
                     VPAELEISSETPGSVHLEVMGASSALKPNALSSVSVVFDLKQVVRPGERTFTVDAETV
                     VLPAGVKLVRAMPSQLRLRFEQRVTREVPVQVRFSAPPPDGYRIQRLEADPPRVWIIG
                     PESRVVQIENAETDPVDLGAVVGERQFQVHAFVDDPRVRFSAEPTVTVKLLMEKVPSG
                     EAR"
     gene            complement(7883..8746)
                     /gene="cdaA"
                     /locus_tag="VLE22_24695"
     CDS             complement(7883..8746)
                     /gene="cdaA"
                     /locus_tag="VLE22_24695"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HSB17670.1"
                     /translation="MTFLFWPFPASLPKLSFTAVIDILLVAFLIYQFFMLVRGRRSAQ
                     VLTGVAVFLVTYLLASRFGFELLRSILATVVPYTAFVLIVLFQSEIRRALLRLGRRRW
                     LGFGGRLQRLESADEILLALGQLAEQKTGALIVIEKDSGLRTFVESGVPLDAHLSRDI
                     LLSIFQPGGALHDGAVIVQRDRVAAAACFLPLSMNPALSRKLGTRHRAAIGLTEESDC
                     LAIVVSEETGRISVAAYGEIEIGVTLRHVAERFAEHFGRRAPGAALAGEEAGDIPLNS
                     GEPHWTRSREE"
     gene            complement(8780..9892)
                     /locus_tag="VLE22_24700"
     CDS             complement(8780..9892)
                     /locus_tag="VLE22_24700"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB17671.1"
                     /translation="MRRREFLGLLGAAGLSAKEPPAPPVAVAKCPSYNEDLNTVLSTM
                     FDQIGGLSGFVRGKTVTVKLNLTGSPGDRVEGRPPAVTHFSHPKMAGSLAHLLGRAGA
                     RRIRFVESCWATAGPLEEFMLDSGWNVRALKNAAPGVEFENTNALGKSKSYGRFRVPG
                     GAYIYPAYDLNKAYEDTDVLVSMAKLKEHATCGVTLALKNFFGITPASIYGDDTREDE
                     PNESPTKGRVEVLHNGRRRPPKSAPQELDPASSREAGYRVPRIVTDLVAARPIDLSII
                     DGIETIAGGEGPWVRGVRRVHPGVLVVGTNPVTADAVATAIMGFDPRAERGTAPFLTC
                     DNTMRLAEAVGIGSTDLRKIEVRGVAIDAARFRFRG"
     gene            complement(9897..11261)
                     /locus_tag="VLE22_24705"
     CDS             complement(9897..11261)
                     /locus_tag="VLE22_24705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17672.1"
                     /translation="MVFALILLAAVADWVPVRWISGEPKSLELLAETPMNCLMLEEAH
                     WSAELSAAAAGRGLVTLGVIRPGSDTAAAAGRAMAARLNGVVLEGDFETEVAQYVREG
                     VGKAGGTVIELSPRGAVPFRDAGVITGTSQGVWPGLHAQEDGAVRAAPTGTPWVDTNT
                     GFLRFARAATSATIWIAYEPPKKGIIQPTRYIQAICDAALTGARWIVTLDGEFARRLL
                     ARDAVALKTWREMGAYLRYFENHKEWRQYEPHAGLAVVQDADSGALLAGSILDMVAVK
                     NTPVRPVPRWKLEAESLKGSKMAVSVDRESLTPEQTEVLRGFARAGGTLLSGPAGWRF
                     PAARRDQVTMGKEDVERLDALWKGVNSMVGRSNLGARLFNVSSMLTNLLQGPGGKPVV
                     LHLANFSGYPVENVTAQFPDAYKRAKLLTPDAAARDLELYPTEDGTAVDIPEVPGFAT
                     LILD"
     gene            11344..12255
                     /locus_tag="VLE22_24710"
     CDS             11344..12255
                     /locus_tag="VLE22_24710"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HSB17673.1"
                     /translation="MKVRLAIALVVLSFLVVAAGVFYPPVRLFGLVLIGRSPHCSMAQ
                     AVASEHHLRQLIDSKDRILAASRLIETDSKGFRLWETPMGRYWIPPNSDYVLPWNLSE
                     QHNKIYGTGEKAVGRGDIVLDCGANVGVYVRESLEAGAKTIVAIEPGPENIECLRRNF
                     PAEIASGKVIVYEKGVWDKEDLLTFYMDPKNPAGDSFVIRLQGDIEKQQMPVTTIDKL
                     VEELKLERVDYIKMDIEGAEQKALAGGRQTIAKFHPRLALSTYHRPDDPEKIPELVRQ
                     AWSGYRIECGPCAYANGIIRPDVLFFR"
     gene            12337..12942
                     /locus_tag="VLE22_24715"
     CDS             12337..12942
                     /locus_tag="VLE22_24715"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB17674.1"
                     /translation="MKTGATSAELPPREAIRRAAIRLFSQKGFAATSTREICERAKVT
                     KPVLYYHFGSKEQLYRELVVDAGNESLKQMLLAASRARGAREKLVDVLAADFEVTRRD
                     PAMAQMFFHMLFAPRKETPTVNYVQVGMEWLRMIEGIASEGVHNRELKGKPREIAEAL
                     LGIHMIYTMGYLLLGEPDLDRKLAKRIVNLMLQGCGNATGR"
     gene            12966..14306
                     /locus_tag="VLE22_24720"
     CDS             12966..14306
                     /locus_tag="VLE22_24720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB17675.1"
                     /translation="MRHLVLPCLLFALLAPVWAESAAEEKSELTIEEAVRIALDRYPE
                     VGKARAAADVLKGRIREVRAQALPEVNINSNYTRWRDPSLLNASGLDKFPEELRRALV
                     PTPVNLFDYAVTVKQPLYTAGKVGTALRLASIEAEGASSDIDRAQQDLAISVVKAAYD
                     LMTAERLRDLVAETQEQRRRQLEIARTRYRNGVATEVDVLRSEVAVANVTPELVRAEN
                     GIRQARALVNFYLVRPIEFPTRVRGDFQEQRWEQTDLDSLTTEAFRRRPELVRLRISE
                     RSAAAQLDLAKAENRLRLDFNANYGIMSRLPENLADPTYARWTAGVNFTFPVFDGFKR
                     SGLVWQATANQRAAHLEREKVEQQIRLGLQQGLDEITAARETITAARANQNQAERVVS
                     MMEDNYKYGAATTLDVLEAQNALTLARMNLLRGLRDYSVARASLKWTIGANPWE"
     gene            14311..15480
                     /locus_tag="VLE22_24725"
     CDS             14311..15480
                     /locus_tag="VLE22_24725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB17676.1"
                     /translation="MSSKARTERGTANGKQIWLAVPAVTVLLLSGCQGKQAVTSVKAT
                     EPTPVRAAVVEVKTEAFSASVAVTGTLVSTSLVDVKAETTGRVLRFPKEEGDRVAAGE
                     AVIWVNDENYRLAILQAESAVKVAEAAADRARVLGAHSQSELERARNLIKSGGITDKD
                     FKAAELAEKDAQAQIAVAEAQLAEARAALAVAKKRMADTVIHAPVAGEIQRKHINPGA
                     YVEPTTAVFTIVNNSRLELRSPVPTADLAPIRTGQRTTFAVNAYPGETFEGRVLEINP
                     AVDAQTRSATVSIRADNRGGRLKAGMFATGEILTGVVAQAILVPAASVYRDDRSAKSS
                     FVYVVENGKAIRRPVRIGRETDSRLEIVEGLKPGDLLIAEQSIELADGVPVQAGK"
     gene            15487..>17653
                     /locus_tag="VLE22_24730"
     CDS             15487..>17653
                     /locus_tag="VLE22_24730"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSB17677.1"
                     /translation="MFLSDLSIKQPVLATMLAVSLMTLGIYSYRELAVDLFPDVEIPV
                     LTVQTLYPGASPETVEREVTKRIEEAVNTIPGVRHISSTTTEGLSAIVIEFRLGTNIH
                     TAQQDAQAKINAIRADFPKDMEEPVIQRIDFNAMPIVSVAVESETADIKALSSLAEKV
                     IKKRLENIPGVGQVTLVGLARREIQVLADREKLKAFGLTYAEVAGALQRENVDLPAGK
                     LEEGKREPLVRVAGKFKSVDGFQNLIVAVRNGRPIYLPEVARVVDGIEERRSASLMDE
                     RSGLSLDIVKQSGANTVEVADGVEHAVARLNAELPPQIRLRSVVDRSIFIRESVEDVQ
                     VTLVLGGLLTVLIVFLFLNSWRSTVITGLALPVSVISTFIIMAALGFTLNILTLMGLS
                     LAIGLLIDDAIVVRENIVRHMHEGVDHYTAAREATAEIGLAVMATTFTIIAVFVPVAF
                     MGGIIGRFFFQFGITVGFAVLVSLYVSFTLDPMLSSRWYDPAADPSVPRSWFGRGLGK
                     LNDQLDRLRGVLASSLHWSLSHRWAIMLIGTVAVFSSFFLFGKLGSAFMPEADPGEFR
                     VNYKARPGVSLERSEEIARELVSEIRKSPGIEYTYTTIGGSGGRSINEGSIYVRLGHD
                     RRARPHFLVIMAGLRERLKRFRGLRTSVGNADEFAGESKPIQLSFRGDDLARLSVLSE
                     RLLKEVRQVRGAADVDTSEEEQRPEVRVLVNRNAAGDLGL"
BASE COUNT         3280 a         5747 c         5358 g         3268 t
ORIGIN      
        1 cgcgtgaccc gtttccgcta tcagccacct tcaactcagc ccccgagcta taattccccc
       61 ttgatgttgc cggatctcct ccatctgcac acttacttcc tgtttccgtt ctccatcgac
      121 aaacaggcgg tgctggagga ccaccgcgac ctctgggcga agtacccccg ttggatcgac
      181 ggtctggatg agtggatcgc cgtgcatcgc acgcgggcca gctccacagt ggcgcagcac
      241 ctcggcccct ggcggcgcgc cgcttacagc cgcttcgatc ttgactcccc cgcctaccag
      301 gacatggtct tctttcaccc tatcgtccgc cgagtgttct tcgacaccac cgacgcagcg
      361 ggcggagaaa aggaagccct cctgcgttgc tacacaatcc cccttggagc ggacggaaag
      421 gtatggctgg aggcggaaga caggaaagga cgcagcgcca gggttcagat caccgagctt
      481 cgcctcttcc tgttcgcgaa cggcatcggc attctgtcga tcggcgtcga ggccttccat
      541 atctccgccg ccgatgcgct ctggatcaac gagagccttc gcaaggtgta cccgtcgagc
      601 ggccgccagc ttcgcgaggc gcgggtcccg gaccggttga cgatcgctat cgagcgcggt
      661 ggcgttcgtg agattgtcgc cgaggagcgc ttcgaaaagt gcgacatgat cggctttctg
      721 ccgcctttgt cgagcacgat cgcctcgctt ctctacttca ccgactatga gcgggaggag
      781 tatgagcccg ttctcgacga acggatgatc gtatactcct actgcgcggt ggaccccgcc
      841 gccatcagtc ccgaattcat cctctccgag gactaccagg tttttctaag ccgcttcctc
      901 tacgtagacc ggtggggtcc cggctaccgt tacgagccgg tcttcacccg cgaggagatg
      961 cggcgtcagt tctatacgcg gtgggcccac caaggcacct attacggctt caccagctac
     1021 agcaacatca cctccaccat cggcaccttc gattgcgatg agcaccagtt gctcgagggt
     1081 tttctcgtcc accgcatgtt cgagacgcgc tactacctga tggctctcgt cgccctcttc
     1141 taccgcgcga ctctcctgga cttctctgag cggaccgctc tcgtctccaa gcagctctat
     1201 ctcgatcagg aggacggacg gatcggcgtc gagaacatcg gcatggccaa cgacctgcgc
     1261 gccgacttcc tgcacttctc caactactgg cacttcgacg agttggccaa caaggacgaa
     1321 gagctcgatc acttcacctt acagtgccgc gaatatcgca tccgccccac gaaagaggag
     1381 gtagaggagg agatcgagaa actcaacgcc tcgctacaca cctatcacca gttccgcaac
     1441 accgaggcca tcaaccgtgt cgcgatgttg agcctgctgt tcggcgccgg cgctgtgctg
     1501 acgggcttct tcggaatgaa cttcggccac cgcttcgggc ggctcttctt cgagccggac
     1561 cagtcgacgc tgccattcca ctacgcggcc gtcgtagcgg ttctgttgct cgccttcggc
     1621 gcgcttgcct tcggtatctt cgttgtgatc gccaactggt ccgactacaa ggagatcttc
     1681 atgttccggc gcggcagcaa tcggcttcct ggcgacgtcg gtagtctccg ccgccgcccg
     1741 cccccagtca gccgcccgca gaactgacgt ccctcagtgc agaggccccc agcccgtgag
     1801 gcctacgagc gcatgttgag tggccggcca ggcgcgcctc tcgtagtctt cacgatgagc
     1861 agccagaacg gagaaatcag tgaggcccca aagcgagcgg ctggccaaca tgtatactgg
     1921 ccgtaggctg gcgccacaaa atcgaattct gggctggatc tttaccctcg gcctggaggc
     1981 atcgagatgg aaaacctgtt gcgcgacgcc cggttcagca ccctgatatt gcttaagacc
     2041 ccggccttca ccgccgtggc tgtgctctcc ctggcgttgg ggattggcgc gaatacaacg
     2101 atcttcagcg tcgtcaacgc aattcttctc cggccgcttc ccttcaaaca acccgaccgg
     2161 ctggtgacga tctccgaaag aaaccgggag aaaaatcaag ggtcgcgcaa tccgcggctc
     2221 tcgacggcca tcgaatggag agagaagacc cggtccttcg cacaagtgga gctcgccgtc
     2281 ccgtacaccg agtcgactac gctctccggg caagcttggg ccgagcaggt catcattcaa
     2341 tttctcgacc gggactgttt ttcgctgctc ggcagcaaac cgatcctcgg ccagaccttc
     2401 cccgccggcg aggtcatcga caatacgagc cggaacatcc tgatcagtca tggtttctgg
     2461 cagcggcgct tcggaggaga ccagaaggtg atcggtcaga cgctgatcgg cggcgatggt
     2521 ccgtggacca tcgtcggcat catgcctcct ggctactgga tatttccttg ggcgaaggac
     2581 gcggacgtct tctcggcagt gagtctgaca aagaacaagc tgacgcccga gacgcgatgg
     2641 ttcagtgtcc tggcccggct caagccggac gccaccctgg cccaggcgca agccgagatg
     2701 gacgtattcg cccgccggat cgagcagcag tatccgcaga ccaacaaagg ctgggatgtg
     2761 aaggtcgaac cccttcagca gtatttgttc aggggatggg gagacgtgct gtacctgctg
     2821 ctgggaacgg ttgggtttgt tctgctcatc gcgtgcgcga acgtcgccaa cttgctgctg
     2881 gcccgcgccg caatgcggca gaaggagatt gccatccgcg cctcgctcgg cgccggtcgg
     2941 tggcgcctga tccagcaact gctcaccgag agcgtggtcc tggcggtcgt gagcggtctt
     3001 ctagggatac tggtgagtct ggccggcatc cggatttttg ttgcattggc gccggaatgg
     3061 ttcccgcgcg ccgaggagat cggaatcgat ttccgggtgc tgggcttcac gctcggcgtc
     3121 tccctgctga ccggaatcct gttcggactg gctccggcgt ggaaagcctc ccggcccgac
     3181 ctgaccgaat ccctgaagga aggggggagg agttcggggg gagagttgcg tcagtacggc
     3241 cgcagcctgc tggtggtgtc cgaggtcgcg ctggccatgg tgctgctgat cggcgccggg
     3301 ctgatgatca acagtttcct tcgcctgcga ttcgccgagc ctggctacaa tcccaggaat
     3361 ctgctgacgg cgcaaatcca gttggatggc cccaggtaca gggaactcct cgagggtgac
     3421 atgaagcgcg taacgccccg ggtggatatt ttctttgagc aggtgctgga gcggcttcaa
     3481 ggtcagcccg gggtcgaggc ggcggccgtg gccagtcgct tcccaatgca gcgggcgttc
     3541 cggatcattg ggcgtcccga acctccgacg aaccagcaac cggtcggcgc cgtgcttgaa
     3601 gtcactgccg gctacttttc cacgatgcaa gtcccgctga gaagaggccg gactttgact
     3661 gagggggaca accagagcgc gtcctgggtg gtggtggtca acgacactct ggcaaagcgt
     3721 tactttccgg acgaggatcc gatcgggaaa acgatccaat tgaaatttgg cgacacttcg
     3781 ggggtcaatc ttgatgagaa taagccgcgg gagatcatag gtgtggtggg aagcgtcaag
     3841 tattggggct tgagcaacga tccaccgccc gcgctgtacg tgccgtaccg ccaacacatg
     3901 tcggactatc ccggcggcac gtcgcagaca cgtctcggaa agacgctgct gctccgcacg
     3961 aaatccgacc cgatgcacct tgccggtgcc gtgcggcgcg tggtggccga agccgacaaa
     4021 gaccagccgg tctccgagat gacgagcatg gagcagaccc tctcggagtg gctggctccg
     4081 cagcgctttg cgatgcaact gtttgggatt ttcgccgctc tggcagtgat tctggcagtg
     4141 gtgggcatct atggcgtcat gtcgtactcc gtgagccggc ggacgcatga gatcggtctg
     4201 cggctggcgc tgggtgcgga acgggacgac gtcctgaagc tggtccttcg gcgggggctg
     4261 aagctctcgc tgatcggcgt agcgatcggg atcgccgggg cactggcgat gacccgcctt
     4321 gtctcgggat tcctgtacgg cgtgaaggcg agcgatcctt tgacgttcgc ggctgtttct
     4381 ctgctcttga ttctggtggc gctggcggcg agttacatac ccgcccggag ggcaacgacc
     4441 gtggacccga tggccgccct gcgctacgaa taaagaaatc tccccgtcgc tctcgctggg
     4501 gcaggtcccc ggcctgcggt ggcctctcag gccgctcacc gccatctgat gtgatgctcc
     4561 tcgcaccagt cgaccgcgat ctgccttaag gcctcgctgc ggaatgcaaa ccaagccgat
     4621 tcgatgttgt gtgcccgcac gcggtgcttg aaatggcgga atgcgccgcc gccgtggatg
     4681 gcgctcagaa gatcctcgcg aatggaatcg cgcttcactg aattcgcgaa gtcccgcatg
     4741 atcgcccact cgtgaacgtc gaacttagtg ggaagcccca ggaaccgatc agtggacaca
     4801 attcgcttgg cggcctgcca ttcctcctcc tgccaatcat gaaggttcgg ctcttggccg
     4861 ccagcgttct cttccgcttc gcgcaaaagc ttgtaagaga cggtcaccac ctgcaaagtg
     4921 tcgagatcta ggaaggaaga agattcgtcg aactgcatct ccagtgcttc gacgatatcg
     4981 ctgagtagca cagtcactga cacagcttta atgacagcat agctctccgc gactcccctg
     5041 ggtggctccg tgagcccctc cgggctgtcg gtggcacggg ctgtggggca ggatctcatc
     5101 ctacggcggt ctcccaggcc gctcgatccc cggcccagcc gtgccctccg cttgcttcgc
     5161 ctacctcttc gtcaagtccc ggatgtagat gtccttgaac cgcatgtcgc cctcgccgcc
     5221 cgaatgcagt tgcagggcga tgtacccgtc gaacgacttc ggcgttggat cggtgaagtc
     5281 gatcatccgc actccgttca ggtacgaaac atacctgttc ccctccacct tcatcagcat
     5341 ctcgttccag tcgtcgggct taatcagcgg ctccagctcc ggcgctggcc agacgatcca
     5401 cgcccgcccg tcgccgtaaa tcccgccgct gtggcgtccg accgtcttgt cgatctcgaa
     5461 ctgaagcccc tgcgatacgt ccgccgtgcc gggctcgaag tcggtgtgga agaagacgcc
     5521 gctgttgccc ttcgcttcgc acttgaaccg gatgaacatg tggaagtctt tgtacttctt
     5581 ttccgtccgc aggtaaccgt attccttcgt aatgccttgt ccgtggatcg ttgcgttttc
     5641 aaccaaccac ttctcgttgc ccaccttgac ccagcccgtc aggtcttgcc cgttgaacag
     5701 cgacacccag tcttccccgg gcaataccgg cttgccgccc tgggcccacg ccatcgtaac
     5761 gccggcgaat agaagaagaa ttcgtgcgaa catgctacct ccctttggat ggaatcccgt
     5821 gacgggattg aattgacatg atactcgaag cccggctcgt caggacccga ctgctccaat
     5881 ggcccgccgg atcgactccg cgatccgctc tgcgaacagc tccacccgcc cctcatccac
     5941 gccttccacc atcacccgcg ccagcagttc tgttcccgaa tagcgcacca ggatccgccc
     6001 cgcgtcgccg aaggcctccg acgccgcccg caactccgcg gccacttccg gcaactcttc
     6061 gagcggcgcc ttctctcgaa ctcggatgtt gaccagccgc tgcgggtgta cttcgaagtc
     6121 ccgcgtcagt acgtccagat ccttccccgt ttcgcgcatt acctccaaca cacgcaacgc
     6181 tgtcagaagg ccgtcgcccg tggtggcgta ctgccggaag atgacgtgcc cggactgctc
     6241 gccgcccagc atcgcgccac accgcagcat ctcctccaac acgtacttgt ccccgaccgc
     6301 cgttcgcagc atccgtatgc ccagccggtg cagcgccttc tccagaccga ggttcgccat
     6361 caccgtcgat accaccgttg gctctccgtc cggacccggc agctcgcccc gctccaacat
     6421 atgccgcgcg gtaatcagga ggactccgtc cccgtttacc gccctcccgc tgtgcgatgc
     6481 gaagatcgcc ctgtccgcgt ctccgtccag cgccacccca atgtccgccc cgagctccac
     6541 aacagcacac cgtaacccgt ccacatgcag cgctccggaa tcgaggttga tgttccggcc
     6601 gttcggcgag caatgaatcg cgtccacgcg cgcccccaat ctcccgaaca gctccggcgc
     6661 aaggtgggag gccgcgccat tggcgcagtc ggccaccaca cgcatccctt cgagcgttcc
     6721 cgggaatgtc gaggcaagga agtcgacgta gctcgcgtcc aggcccggat cttctcgcaa
     6781 ctccgcgggc tccgctcgca cgccggcctc cagcaatgcg aagatctccc gctcgatcgc
     6841 cttctcctcg gcgtccggga acttatagcc cgagtggccg aaaatcttga ttccgttgtc
     6901 ctggtaagga ttgtgcgacg ccgaaatcat cacccccgct acgaacgggc ctgtgcgcgt
     6961 cacgtaagcc acgcccggag tggtgatcag ccccgcgaac cgcggccgtg cgccgcctcg
     7021 aacgagcccc ccagccaact gcgccgcgat ccattggctc gattcccgcg tgtcgatccc
     7081 gacgagcact tccggatcct gtctcaggcg cgttgccagg tgtccaagcg cctcgccgct
     7141 ggcgccaacc gtgagcggat ccagcggaaa cgttccggcc tcgcctcgaa ttccatcggt
     7201 gccgaaaagt cgcttcggca tcaacgcgcc tcgccggacg ggaccttctc catcagcagt
     7261 ttcactgtca ccgtgggctc ggcggaaaag cgaacgcgcg gatcgtcgac gaacgcgtgg
     7321 acctgaaact gcctctcccc cacgacggcc cccagatcga ccggatcggt ctccgcgttt
     7381 tcgatctgga ccacgcgact ctccggaccg atgatccaca ctcgcggcgg gtccgcttcc
     7441 aggcgctgga tacgatagcc atcgggcggt ggcgccgaaa accggacttg caccggcact
     7501 tcgcgggtca cccgctgctc aaatcgcaac cgaagctggg agggcatcgc tcgcacaagt
     7561 ttcacacccg ccggcagtac tacggtctcc gcatccaccg tgaacgtccg ctcgcccgga
     7621 cgaacgacct gcttcaggtc aaacaccacc gataccgacg agagcgcatt tggtttcagc
     7681 gccgaggacg cccccattac ctcaaggtgt acgctgcccg gcgtctcgga gctgatttcc
     7741 agttctgcgg ggacatttcg gaattccagc ggcgccgaaa tggaggtcgc cagttgtggt
     7801 tctcccacca ccgcgagcca caatgctacc gccagcgcca gcgataggat cttccagccg
     7861 atgttctcga gcaggagccg cttcattcct cccggctcct tgtccaatgc ggctcaccgg
     7921 aattcagcgg gatgtcgccc gcctcttcgc cggccagggc cgcgcccggc gccctccgcc
     7981 cgaagtgttc cgcgaaacgc tcggcgacat gccgcagcgt gacgcctatc tcgatctcgc
     8041 cgtaagccgc caccgagatg cgccccgttt cctcggacac cacgatcgcc aggcaatcgc
     8101 tctcctcggt caagccgatc gccgccctgt gccgcgtccc caacttgcgc gaaagcgcgg
     8161 ggttcatgct cagcggcaga aaacaggccg cggccgccac ccggtcccgc tggacgatca
     8221 ccgctccgtc gtgcagagcg ccgccgggct ggaaaatgct caacagaatg tcgcgggaca
     8281 gatgcgcgtc gagcggcact ccgctctcga caaaggtccg cagcccgctg tccttctcga
     8341 tgacaatcag cgcaccggtc ttctgttcgg ccagttggcc cagggccagc aggatctcgt
     8401 ccgccgattc gagccgttgc aggcgtccgc cgaatccaag ccaccgccgg cgcccaagcc
     8461 ggagcagcgc ccgccggatc tccgactgga acagaacgat gagcacgaag gccgtatacg
     8521 gaacaaccgt tgccaggatc gagcgcaaca actcgaagcc gaaccggctt gccagcagat
     8581 aagtcaccag gaatacggcg acacccgtaa gtacctgggc cgagcgccgg ccccgcacaa
     8641 gcatgaaaaa ctggtagatc aggaaggcga ccagcaggat gtcgatgaca gccgtgaagc
     8701 tgagctttgg aagcgaggct ggaaacggcc aaaagagaaa tgtcatcaat tccgagcttc
     8761 tatcttagcc cggacccgat tatccgcgaa accggaatcg cgccgcgtcg atcgctaccc
     8821 cgcggacctc gatctttctg aggtccgtgg acccgatgcc gaccgcctct gccagtctca
     8881 tcgtgttgtc gcaagtgaga aacggcgcag tgcctcgctc ggccctcggg tcaaacccca
     8941 tgatcgcggt cgccaccgcg tcggccgtca ccgggttggt ccccaccacc agcactcccg
     9001 ggtgaactcg ccggacgccg cgcacccacg gaccctcgcc tcccgcgatc gtctcgatgc
     9061 catcgatgat cgacaggtcg atgggtcgtg ctgccacaag gtccgtcacg atgcgcggca
     9121 cccggtatcc cgcctcgcgg ctcgacgccg gatccagttc ctgtggcgcg ctcttcggcg
     9181 gccgccgcct gccgttgtgg aggacttcca cgcggccctt cgtgggactc tcgttgggtt
     9241 catcctcccg cgtgtcgtct ccgtagatcg atgccggcgt gatcccgaag aagttcttca
     9301 gcgcgagcgt gacgccgcag gtcgcgtgtt ctttcagctt ggccatcgac accagcacat
     9361 cggtgtcctc gtacgcctta ttgaggtcgt acgcgggata gatgtatgct cctccgggta
     9421 cccggaatcg cccgtagctc ttgctctttc cgagcgcgtt cgtattctcg aactccacgc
     9481 cgggcgcagc gttcttcagc gcgcgcacgt tccaccccga atccagcatg aactcttcga
     9541 gcggccccgc cgtcgcccag cagctttcga cgaaacgaat tcgccgcgcg ccggctcgtc
     9601 caagcagatg cgccagcgac ccagccatct tcggatgcga aaagtgcgtc accgccggcg
     9661 gacggccttc tacacggtcc ccgggactcc cggtcaggtt caacttcact gtgaccgtct
     9721 tcccacgcac gaacccgctc agcccgccaa tctggtcgaa cattgtggac agaacggtgt
     9781 tcaggtcttc gttgtacgag gggcacttcg ccacggcgac cggcggcgcc ggcggttcct
     9841 tcgccgacag ccccgctgcg ccgagcaatc ccagaaattc gcggcgtctc atactattag
     9901 tctagtatca aggtcgcgaa accgggcact tccggaatgt cgacggccgt gccgtcttct
     9961 gtcggataca actccagatc gcgcgcagcc gcgtcgggcg tcagtaactt cgcccgcttg
    10021 tacgcgtccg gaaactgcgc cgtcacgttc tccaccggat agcccgaaaa gtttgccagg
    10081 tgcagaacga ccggcttccc tccaggaccc tgaagcaggt tcgtcagcat gctcgacacg
    10141 ttgaacagcc gcgcgccgag gttgctgcgc ccgaccatcg agttcacgcc cttccacagc
    10201 gcgtccagcc gttcgacgtc ctccttgccc atcgtcacct ggtcacggcg cgcggccgga
    10261 aaccgccagc ctgccggccc ggagagcagc gtgccgccgg cccgcgcgaa cccccgcagc
    10321 acttcggtct gctccggcgt gagcgactca cggtcgacgc tcaccgccat cttcgacccc
    10381 ttcagcgact cggcctccag tttccaacgc ggcaccggcc tcaccggcgt gtttttcacc
    10441 gcgaccatgt ccaggatgct tcccgccagc agcgcgccgc tatcggcgtc ctgcacgacc
    10501 gccagcccgg cgtgcggctc gtattgccgc cactccttgt ggttctcgaa gtagcgcagg
    10561 tacgctccca tctcccgcca agtcttgagc gccaccgcgt ctcgtgcgag cagtcgccgc
    10621 gcgaactcgc cgtccagcgt cacgatccat cgcgctccgg tgagcgccgc atcgcagatc
    10681 gcctggatat agcgcgtcgg ctggatgatc cctttcttcg ggggctcgta cgcgatccag
    10741 attgttgccg aagtcgcggc ccgcgcgaac cgcagaaagc cggtgttcgt gtcgacccaa
    10801 ggcgtgcccg tcggcgctgc cctcaccgcg ccgtcctcct gcgcgtgcag ccccggccag
    10861 acgccctgtg atgtgccggt gatcacgccc gcatcccgga acggtacggc gcctcgcggg
    10921 gacagttcga tcactgtgcc tccagccttt ccgacgcctt ctcgcacata ctgcgccact
    10981 tccgtctcga agtcgccctc gagcacaacg ccgttcagtc tcgccgccat cgcccggccc
    11041 gccgccgctg ccgtgtcgct tcccggccgg atcactccga gcgtgacgag tccgcgcccc
    11101 gccgccgccg cgctgagttc cgccgaccag tgcgcctcct cgagcatcag acaattcatc
    11161 ggcgtctccg ccagcaactc gagcgacttc ggctcgcctg agatccaacg caccggcacc
    11221 caatcagcca ccgcggccaa caggattaat gcaaacacca taccctcact gtacccgttc
    11281 tttctctcca agagcccggc accagggttg ctgtctgggg taagtgatat catctgaaaa
    11341 acaatgaagg ttcgtctcgc gatcgcgctg gtagtgttgt cgttcctcgt agtggccgct
    11401 ggcgtctttt acccgccagt ccggctgttc ggactggtgc tgattggccg cagcccgcac
    11461 tgctccatgg cgcaagccgt cgcgagcgag caccacttgc ggcagttgat cgattccaag
    11521 gaccgtatcc tggctgccag ccgcctgatt gagacggact cgaaagggtt ccggctgtgg
    11581 gaaacgccga tgggccgcta ctggattcca ccgaacagtg actacgtgct gccctggaac
    11641 ctctcggaac agcacaacaa gatttatgga acgggggaga aagcggtggg tcgcggtgac
    11701 atcgtgctgg attgcggcgc aaacgtcgga gtttacgtcc gggagtcgct ggaagccggt
    11761 gcgaagacga tcgtggcgat tgagccgggt ccggagaaca tcgagtgcct gcggcgcaac
    11821 tttcccgccg agatcgcaag cggcaaggtg atcgtgtatg agaagggcgt ctgggacaaa
    11881 gaggatctgc tgacgtttta catggatccg aagaatccgg ctggtgacag cttcgtgatc
    11941 cgcctccagg gggatatcga gaaacagcag atgccggtga cgacgatcga caagctcgtt
    12001 gaggaactga aactggagcg ggtcgactac atcaagatgg acatcgaggg tgccgaacaa
    12061 aaggctttgg ccggcgggcg gcaaacgatc gcgaagttcc acccgcggct ggccctgtcc
    12121 acgtaccatc gtccggacga tcccgaaaag attcccgaac tggtccggca ggcatggtcc
    12181 ggataccgga tcgagtgcgg accctgcgca tacgccaacg gcatcatccg tcccgacgtg
    12241 ctgttcttcc gctagacaaa acttaagact ttttcatgcg attttccgga acccgatggc
    12301 aaccgtagct cgtattttgt atactgagcg gtaagtatga aaacgggagc gacttcagcc
    12361 gagctgccgc cgcgtgaggc aatccggaga gcggccatcc ggctcttctc acaaaaagga
    12421 tttgcggcaa cttcgacgcg agaaatctgc gaaagggcga aggtcacgaa accggtcctg
    12481 tactaccact ttgggagcaa ggagcaactc taccgggaac tggtcgtcga tgctgggaac
    12541 gaatcgctca aacagatgct gctcgcggcc tctcgggcca gaggggcgcg ggagaaactc
    12601 gtggacgtgc tggcggccga ctttgaagtc acgcgccgtg acccggccat ggctcaaatg
    12661 tttttccaca tgctcttcgc tcctcgaaaa gaaaccccaa ctgtcaacta cgtccaggtg
    12721 ggaatggaat ggctgcggat gatcgagggt atcgccagcg aaggcgtcca taaccgggaa
    12781 ctgaagggaa agccgcgcga gattgccgag gcgcttctcg gcattcacat gatttacacc
    12841 atgggatacc tcctcttggg cgaacccgat ctggacagga agctggcaaa gcgcattgtc
    12901 aacctgatgc tccagggctg cggcaatgct accgggcggt aaaggcgtac tggataacat
    12961 tttctatgcg gcacttggtc ttaccgtgtt tattgttcgc tctccttgcc ccggtgtggg
    13021 cggagagcgc tgctgaagag aagagcgaac tgacgatcga agaagcggtg cggatcgcgc
    13081 tcgaccggta cccggaggtg ggcaaggcga gggctgccgc cgacgtcctg aagggccgca
    13141 tccgcgaggt gcgcgcgcag gcccttccgg aagtcaacat caactcgaac tacacccgct
    13201 ggcgcgatcc gagtctgctc aacgcgtccg gtctcgacaa atttcccgag gaactgcgga
    13261 gagccctggt acccacgccg gtgaacctgt tcgactatgc cgtcaccgtg aagcaaccgc
    13321 tctacaccgc cggcaaggtc ggaacggcgt tgcggctggc gtccattgaa gccgagggcg
    13381 cctcgagcga catcgaccgc gcgcagcagg atctcgcgat ctcagtggtg aaggcggcgt
    13441 acgacctcat gacggcggaa cggctccgcg acctcgtcgc cgaaacacag gaacagagga
    13501 ggcggcagct cgagatcgca cggacgcgat atcgcaacgg cgtggcaacg gaggtggacg
    13561 tactgcggtc ggaggtcgcg gtagcgaacg tgacgcccga actcgtgcgt gcggaaaacg
    13621 ggattcgcca ggcgcgggcg ctggtcaact tctatctggt ccggccgatc gaattcccca
    13681 cgcgcgttcg aggggacttc caggagcagc gatgggagca gaccgatctc gactcactga
    13741 cgaccgaggc gtttcgaagg aggcccgaac tggtccgcct gcggatcagt gagcgcagcg
    13801 cggccgccca acttgacctc gcgaaagctg aaaataggtt gcggctggat ttcaacgcca
    13861 actacgggat catgtcccgg ctgcccgaga acctcgccga tcccacgtat gcccgctgga
    13921 cggccggcgt gaatttcacc tttccggtct tcgacggctt caaacgaagc ggcctcgtgt
    13981 ggcaggcgac cgccaaccag cgagcagcgc acctggagcg ggagaaagtt gagcagcaga
    14041 tccggcttgg cctgcaacag ggcctcgacg agatcaccgc cgcgcgcgag accatcactg
    14101 cggcgcgggc gaaccaaaac caggccgagc gcgtggtgtc gatgatggaa gacaactaca
    14161 agtacggcgc cgcaactaca ttggacgtgc tggaagcgca gaacgcgctg acactggcgc
    14221 ggatgaactt gctacgtggg cttcgagatt actccgtggc ccgagcgagc ctgaagtgga
    14281 cgatcggcgc gaatccgtgg gagtgagaac atgagcagca aggcaagaac cgaaaggggt
    14341 acagcaaacg gcaagcagat ctggttggcc gtgccggcgg tcaccgttct ccttctatcg
    14401 ggatgtcagg gaaagcaggc agtgacctcg gtaaaggcga ccgagccgac tccggttcgg
    14461 gcggccgtcg tcgaggtcaa gacggaggcg ttctccgcct ccgtggccgt gaccggaacg
    14521 ctggtttcaa cgtcgctcgt tgacgtgaag gcggaaacga caggccgcgt gctgcgcttc
    14581 ccgaaagagg agggcgatcg cgtcgcggct ggtgaagcgg tcatctgggt caatgatgag
    14641 aactacagac tcgccattct tcaggccgaa tccgcggtga aggtggcgga ggcggcggcc
    14701 gatcgcgcgc gggttctcgg cgcgcacagc cagtcggagc tggagcgggc gcggaacctg
    14761 atcaagtccg gcggtatcac ggacaaagac ttcaaggcgg cggagctcgc ggaaaaggac
    14821 gcgcaggcgc agatcgcggt ggcggaggca cagctcgccg aggcccgggc ggcgctggcg
    14881 gtggccaaaa agcgtatggc ggacacggtg attcatgctc ctgttgccgg cgagatccag
    14941 cgcaagcaca taaacccagg cgcctacgtc gaacctacga cggcggtgtt cacgatcgtg
    15001 aataacagcc ggctggagct gcggagtccg gtgcccacgg ccgatctcgc accgatcagg
    15061 acgggacagc ggaccacgtt cgcagtgaac gcatatccag gcgagacgtt cgagggccgc
    15121 gtgctggaaa tcaaccctgc ggtggacgcg cagactcgtt ccgccacggt cagcatccgt
    15181 gccgacaacc gcggcggccg tctaaaggca ggaatgttcg caacgggtga aatcctgact
    15241 ggcgtcgtcg cgcaggcgat cctcgtgccg gccgcatccg tctaccggga cgaccggtcg
    15301 gcaaaatcgt cttttgttta tgttgtagag aacggcaagg cgatccgccg gccagtgcgg
    15361 atcggccgcg aaaccgacag caggctggag atcgtcgaag gattgaaacc cggagatctg
    15421 ctgatcgcgg aacagagcat tgaactggcg gacggagtgc ctgttcaggc aggaaagtag
    15481 gcgcgtatgt ttctgagcga cctgtcgatc aagcaaccgg tgctggcgac gatgctcgcc
    15541 gtgtcgctga tgacgctggg aatctactcg tatcgggaac tcgcggtgga tcttttcccc
    15601 gacgtcgaga ttcccgtgct gaccgtccag accctgtatc ccggcgcctc gcccgaaacg
    15661 gtcgagcgcg aagtcaccaa gcgcatcgag gaagccgtta acacgattcc gggcgtgcgc
    15721 cacatctcat cgacaaccac ggaaggcctg tcggccatcg tcatcgaatt ccgcctggga
    15781 acgaacattc acacggcgca gcaggacgcg caagcgaaga tcaacgccat ccgcgccgat
    15841 ttcccgaagg acatggaaga gcccgtcatc cagcgcatcg acttcaacgc gatgccgatc
    15901 gtctcggtgg cggtggagag cgagacggcg gatatcaagg ccctctcgtc actggccgag
    15961 aaggtcatca agaagcggct ggaaaacatc ccgggcgtcg ggcaagtgac cctggtgggg
    16021 ctggcgcgcc gcgagatcca ggttctggcc gaccgcgaaa agctcaaggc gttcgggctc
    16081 acgtatgccg aagtcgctgg agcgctgcag cgggagaacg tggatctgcc cgccggaaaa
    16141 ctcgaggaag ggaagcgcga gccactggtg cgcgtggccg ggaagttcaa gtcggtggac
    16201 gggttccaga acctgatcgt ggcggtgcgg aacggccgtc cgatctatct ccccgaagtc
    16261 gcgcgagtgg tggacggcat cgaggagcgc cggagcgcgt cgctgatgga tgaacgctcc
    16321 ggcctgtctc tcgacatcgt caagcagtcc ggcgcgaaca ccgtcgaggt ggccgacggc
    16381 gttgagcacg ctgtagcaag gttgaacgcg gagcttccgc cgcagatccg gctgcgcagc
    16441 gtggtggacc ggtccatctt catccgcgag tctgtcgagg acgtgcaggt gactctggtc
    16501 ctcggcggtt tgctgacagt gctgatcgta ttcctgtttc tcaatagctg gcgtagcacg
    16561 gtgattaccg gcctggcgct gccggtgtct gtgatcagta cgttcatcat catggcggcg
    16621 ctcggattta cgctcaatat cctgacgctg atgggtctgt cgctggccat cggcctgctg
    16681 atcgacgacg cgatcgtcgt ccgcgaaaac atcgtgcgcc acatgcacga gggcgtcgac
    16741 cactacaccg cggcgcggga ggcgaccgcc gagatcgggc tggcggtgat ggcgacgacg
    16801 ttcaccatca ttgccgtgtt cgtcccggtg gccttcatgg gcgggatcat cgggcgattc
    16861 ttcttccagt tcggcatcac tgtcggtttt gccgtgctgg tgtcgctgta tgtgagcttc
    16921 accctcgacc ccatgctgtc gtcccgctgg tacgatccgg cggcggaccc gagcgttccg
    16981 cgctcgtggt tcggccgcgg actcggaaaa ctgaacgacc agttggaccg gctgcggggc
    17041 gtgctggcga gttcgctcca ctggtcgctc tcgcatcgct gggcgatcat gctgatcggc
    17101 acggtggccg tcttctcaag cttcttcctg tttgggaaac tcggctcggc cttcatgccg
    17161 gaagccgacc ctggcgagtt ccgcgtgaac tacaaggcgc ggcccggcgt aagcctggag
    17221 cgctccgagg agatcgcccg ggagttggtt tcggagatcc gcaagagccc gggaatcgaa
    17281 tacacgtaca cgacgattgg cggcagcggc ggccgctcga tcaacgaagg cagcatttac
    17341 gtgaggctgg gccatgatcg gcgcgcccgg ccgcattttc ttgttatcat ggccggcctc
    17401 cgcgagcgtt tgaagcggtt tcgcgggctc aggacttcgg ttgggaacgc cgacgagttc
    17461 gccggcgagt cgaagccgat ccaactgagc ttccgcggcg acgacctggc tcggctgagc
    17521 gtgctaagcg agcgccttct gaaagaggtc cggcaggttc gaggcgccgc cgacgtcgac
    17581 accagcgagg aggagcagcg gccggaagtc cgtgttctgg tgaaccgcaa cgcggccgga
    17641 gacctcggac ttg
//
LOCUS       DATIFQ010000490         4893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1891283, whole genome shotgun sequence.
ACCESSION   DATIFQ010000490 DATIFQ010000000
VERSION     DATIFQ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891283"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VLE22_24735"
     CDS             complement(<1..1247)
                     /locus_tag="VLE22_24735"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HSB17678.1"
                     /translation="MSSGAVSVGPGEEAALTLRFETVRANHLQLDVIGEAPETALREI
                     PGSAFLVSREELAESVPADANEVLRQVPGVNIREDSGPVGMRLNIGIRGLNPDRSRSL
                     LVLEDGLPLALAPYGEPEMYYSPPIDRMSRVELLKGSGSILYGPQTIGGVLNFITPDP
                     PLRPQGELELQGGQYGFFTGRLSYGGTHETAGWYVSLLRKQGDGWRAFYFDINDLTAK
                     VNVDVSTRQRVGLKFGVYDERSNSTYLGLTESQFRQNPGFNAVPDDLLKVRRYSGSVI
                     HQVVLSPRALLSNSFFGYTTGRNWRREDFDRTPRAGVIYLRLVGDPAVPGAAIWLRNS
                     TGNNNREFDVAGAESRLSVEHEALGFRHKLETGVRYVYEEHRDRRIDGANFMALSGVI
                     RSDETRHGKAWSGFIQDRILVTNR"
     gene            complement(1601..3079)
                     /gene="glpK"
                     /locus_tag="VLE22_24740"
     CDS             complement(1601..3079)
                     /gene="glpK"
                     /locus_tag="VLE22_24740"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HSB17679.1"
                     /translation="MQFILSLDEGTTTARAALYDEQGRRIGMESEPVTCLYPESGWVE
                     QDPEEIWRAQLSAAQRTLERGAVKPADVAALGITNQRETTLVWERGAGKVVAPAIVWQ
                     CRRTAGFCTELAAGPAGAIVTRKTGLVVDAYFSASKIRWILEHVPDARRKARDGELLF
                     GNVDTWLLWRLTNGLVHATDFTNASRTMLMDLASGDWDNELLGIFDIPRAMLPRIVPS
                     SGVAGVAAAEHLGAEIPIAGIAGDQQAALVGQACFRHGLSKNTYGTGCFALLHTGDHV
                     PVSQNRLLGTRAASIDTRAQFASEGSIFIAGAAVQWLRDELKLIATAAESEEVAASVA
                     DTGGVYFVPAFVGLGAPHWDSSARGLLTGITRSTGRAHLVRATLESIAYQTRELVEAM
                     EADTGERLAELRVDGGATANNFLLQFQADILGSPVVRPWDIETTALGAAYLAGLATGF
                     WKSVGELESFWRVERVFEPSMNEERRQGLFAGWKRAVSRCRD"
     gene            3124..3528
                     /locus_tag="VLE22_24745"
     CDS             3124..3528
                     /locus_tag="VLE22_24745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17680.1"
                     /translation="MFFRRERPKQVTFSERLDALRKAGFAVESHDAGTVKVSRDRCAA
                     VIADVPGALPRIGKMGVVRGGDIAFLIDGGFQKFFRTPAGKRVPALAEDLQALHAFEE
                     DLRDALGLVSLYNEALGTTCDYHLYDRLEGRE"
     gene            complement(3530..>4893)
                     /locus_tag="VLE22_24750"
     CDS             complement(3530..>4893)
                     /locus_tag="VLE22_24750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17681.1"
                     /translation="HILHGYFYRRKGHLVVKGSVEEVASVKTSRTLTAAGPALLTAAN
                     TIARQIDPTAEPYGTSNEAALRALMEGRAVGDAASAASAFERAVKADPAFGAASAAWG
                     QALTSEKRYAEAAAALREAMRRDPEQPLLWNELGYTEAFAGNLDGAVKALREYEKRAP
                     GDANPLDSLGDVHYYLGHFAEAERFYLQAHEKDASFGGGATGYKAARARLMTGDAAGA
                     TEIFRRFADTRRAAGDPQVDFQQAQWSYLSGRIREAEDQASQFAGKTANPDAAAQAYS
                     QLSVWRLAAGDVTRAREYAARANSNARQPIPRMLAGICGFLAGPPAPAADWRKRAAGQ
                     FSGPAGGAAGRYALAYALLFGRHFDDAAGVLRDLNRTASPIAPEQVPVLLAWALAETG
                     RFAEAAPLVDTYGIPGPGGESTFACLSFPRVFQVRAAVLKKQGKANEASRSLELFRKL
                     AGR"
BASE COUNT          838 a         1683 c         1487 g          885 t
ORIGIN      
        1 cggttcgtca cgagaatccg gtcctggatg aaccccgacc acgccttccc gtggcgtgtc
       61 tcgtcggaac ggatcacgcc cgacaacgcc atgaaattcg caccgtcgat ccgccggtcc
      121 cggtgctcct cgtagacgta ccgcactccc gtctccagct tgtggcgaaa cccgagagcc
      181 tcgtgctcca cgctcagacg gctctccgct ccggcgacat cgaactcccg gttgttgttc
      241 cccgtactgt ttcgcaacca gattgcagcc cccggcacgg ccgggtcgcc caccagccgc
      301 aggtagatga cacccgctct tggcgttcga tcaaagtctt cccgccgcca gttccggccg
      361 gttgtatatc cgaagaagct gttggagagc agtgcccgcg ggctcaggac aacctggtgt
      421 atgacggaac cgctgtagcg gcgcaccttc aacagatcgt ccggcactgc gttgaagccg
      481 ggattctgac ggaactggga ttccgtgaga cccagatacg tcgaattcga gcgttcgtca
      541 tagactccaa actttaagcc aaccctctgt cgtgttgaaa catccacatt cacttttgcc
      601 gtcaagtcat tgatgtcgaa atagaaagct ctccagccat caccttgttt ccgcagcagg
      661 ctgacgtacc agccggcggt ttcatgcgtg ccgccgtacg agagccggcc ggtgaagaag
      721 ccatactgtc ccccttggag ttcgagttct ccctggggtc ggagcggcgg gtcgggagtg
      781 atgaaattca gtacgccgcc gatcgtctga ggaccgtaca ggatcgaacc gcttcccttc
      841 aacagctcca cgcggctcat ccgatcgatc ggcgggctgt agtacatctc gggctcgccg
      901 tatggcgcca gcgcgagggg aagcccgtct tcgagcacaa ggagagagcg gctccggtcc
      961 gggttcagac cccggatgcc gatgttcagg cgcatgccta ccggcccgga atcctcgcga
     1021 atattcaccc caggtacctg gcgcagcact tcgttcgcgt ctgcaggcac gctctcggcc
     1081 agctcctcac ggctcaccag gaacgccgag ccggggatct cgcgcaacgc ggtttcaggg
     1141 gcctccccaa tgacatccag ttgcagatgg ttcgcgcgga cagtctcgaa ccggagggtg
     1201 agcgccgcct cctcgcccgg tcctacggac actgcgccac tgctcacagg gtcaaatgcc
     1261 tctccggtag cggtcaccgt ataccgtccc gggggaagac cgccaacgag ccacgtcccg
     1321 tcggattcag tagtggtaac ctgctgtatt ggtttgactt ccaccctgaa accagccacc
     1381 gaacggccgc ttgggtcaag gaccttgccg cgaattgccc ctgctgtctg ctgtgccccc
     1441 gcgggagaaa ggaccaggca cgaaaccaat gccaggacac acgtccgaaa agtgcaagtg
     1501 agttgcataa gtgaaataag gagtatacag tcttttgcaa ctcgctgtca acaggatttt
     1561 ggcggcgaac tggccttcgg atcgtgaacg gatgcactcc ttagtccctg cagcggctca
     1621 ccgcccgctt ccaacccgcg aacagcccct gccggcgctc ctcgttcatc gacggctcga
     1681 agacgcgctc gacgcgccag aacgattcca gttctccgac gctcttccag aatcccgttg
     1741 cgagcccggc gagatacgcc gcgcccaggg ccgtcgtttc aatgtcccat ggccgcacca
     1801 cgggactccc gaggatgtcc gcctggaact ggagcaggaa gttgttggcc gtagcgcccc
     1861 catccacgcg cagttccgcc agccgttccc ccgtatcggc ctccattgct tccacgagtt
     1921 cccgggtctg gtaggcgatg ctctcgagcg tggcgcggac gaggtgcgcc cgcccggtgg
     1981 accgcgtgat gccggtcagc agcccgcgtg ccgacgagtc ccagtgcggc gccccgagtc
     2041 cgacgaaagc cgggacgaaa tacacgccgc ccgtgtcggc gacggaagcc gccacctcct
     2101 cggactcggc cgccgtcgcg atcagcttca gttcatcccg cagccactgg acagcggccc
     2161 cggcgatgaa aatgctgccc tcgctggcga attgggcccg ggtgtcgatc gaagcggctc
     2221 gcgtgccgag cagccggttc tgcgataccg gaacgtgatc gccggtgtgc aacagagcga
     2281 aacaaccggt gccgtacgtg ttcttcgaga gcccgtggcg aaagcaggct tgcccgacca
     2341 aggccgcctg ctggtccccc gcgatccccg cgatcgggat ctccgcgccg aggtgttccg
     2401 cggctgcgac gcccgccact ccgctcgacg gcacgatccg cggcagcatg gcccgcggga
     2461 tgtcgaagat gccgagcagt tcgttatccc agtcgcccga ggcgagatcc atcagcatcg
     2521 ttcgtgaggc gttggtgaag tcggtcgcgt gcaccaatcc gtttgtcagc cgccagagca
     2581 gccaggtgtc gacgttgccg aacagaagtt cgccgtctct cgccttccgg cgtgcgtccg
     2641 gcacgtgttc gaggatccac cggatcttac tggccgagaa ataggcgtcc acgactaggc
     2701 cggtcttccg ggtgacgatg gcgccggccg gtcccgcggc gagttctgtg cagaaaccgg
     2761 ccgtacgccg gcactgccag acgatcgccg gagccacaac cttccctgcc ccgcgctccc
     2821 acaccagcgt tgtctcccgt tgattggtga tgccgagggc ggccacgtcg gccggcttca
     2881 ccgcgccgcg ctcgagcgtc cgctgtgcgg ccgatagctg cgcccgccag atctcttccg
     2941 ggtcctgctc tacccagccg gattcgggat aaagacaggt gaccggttcg gattccatcc
     3001 cgatgcggcg gccctgttcg tcgtagagcg ccgcgcgggc ggtcgtggtg ccctcgtcga
     3061 gggagaggat aaattgcacc ctggaattat atgacggctc cgcgccgacg ctacaataac
     3121 cctatgttct ttcgacgcga gcggccgaag caggtgacgt tcagcgaacg gcttgacgcc
     3181 ctccgcaagg cgggctttgc cgtcgagtca cacgacgccg gtacggtgaa ggtttcccgc
     3241 gatcgctgtg cggcagtgat cgcggatgtt cccggggcgc ttccccgcat cggaaagatg
     3301 ggagttgtcc gcggcggcga catcgccttc ctgatcgacg gcggctttca gaagttcttc
     3361 cgcactccgg ccggcaagcg cgttccggcc ctggccgaag acctccaggc gcttcatgcg
     3421 ttcgaggagg acctgcgcga tgccctcggc ctggtcagcc tgtacaacga ggccctcggc
     3481 accacttgcg actaccacct ctacgaccgg ctcgaaggcc gggagtaact catcgccctg
     3541 ccagtttcct gaagagttcg aggctccgtg aagcctcatt tgcttttccc tgcttcttca
     3601 gcacggccgc gcgcacctga aaaacccggg ggaacgacag gcaggcgaac gtcgattcgc
     3661 cgcccggtcc gggaatcccg tatgtgtcca ccagcggtgc tgcttccgcg aagcgcccgg
     3721 tctcggccag cgcccaggcc agcagaacgg gaacctgttc gggggcgatc gggcttgccg
     3781 ttcgattcag atcccgcaat acaccggctg cgtcgtcgaa gtgccggccg aacagcaacg
     3841 cataggccag cgcatagcgg cccgccgcac ctcctgcagg gcctgaaaac tgcccggcgg
     3901 cgcgcttccg ccagtctgct gccggcgccg gaggtccagc caggaagccg cagatcccgg
     3961 ccagcatccg ggggatcggc tgccgcgcgt tggagttcgc gcgggcggcg tactcgcggg
     4021 cgcgcgtcac gtccccggcg gcgagacgcc acaccgaaag ctgggaataa gcctgcgcgg
     4081 cggcatccgg attcgctgtc ttcccggcaa actgcgatgc ctgatcctcg gcttcgcgga
     4141 tacgtccact gaggtaactc cattgcgcct gctggaaatc cacctgagga tcgcccgccg
     4201 cacggcgggt gtccgcgaat cgccggaaga tctctgtggc gcccgctgcg tcgcccgtca
     4261 tcagcctcgc ccgtgctgct ttgtagcccg tcgcgccgcc gccgaacgag gcgtccttct
     4321 cgtgagcctg taagtagaac cgctcggctt cggcgaaatg gcccaggtag taatgcacgt
     4381 cacccagcga atccagagga ttcgcgtccc cgggcgcgcg cttctcgtat tcccggagcg
     4441 cctttaccgc gccatccagg ttgcctgcaa acgcctccgt gtaccccagt tcgttccaca
     4501 gcagaggctg ctccggatcg cgtctcatcg cttcgcgcaa ggcggcagcc gcttcggcgt
     4561 accgtttctc agaggtcagc gcttgacccc aggcggcaga cgcagccccg aacgcaggat
     4621 cggcctttac cgcgcgctcg aacgcagagg ccgcagacgc cgcatcgccc accgcgcgcc
     4681 cttccataag tgctcgcaac gctgcctcgt tggacgtgcc gtaaggctcg gctgtcgggt
     4741 cgatctgtcg cgcgatggtg tttgccgcgg tcagcagcgc cggtcccgcc gccgtgagtg
     4801 tcctcgacgt cttcacggag gcgacttctt ccaccgatcc tttcactacc agatgcccct
     4861 tccggcggta gaagtaacca tgcaggatgt gcg
//
LOCUS       DATIFQ010000491         3229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2849869, whole genome shotgun sequence.
ACCESSION   DATIFQ010000491 DATIFQ010000000
VERSION     DATIFQ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2849869"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1294)
                     /locus_tag="VLE22_24755"
     CDS             complement(110..1294)
                     /locus_tag="VLE22_24755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17682.1"
                     /translation="MTFLLGALVALVGITASPGDLPKSRPIHDYDSPYFARARATVLD
                     RSAEPAKRITAMRELVIRDATSGREIIAEALKDRNARVQKEAAEWLAIYGDSRGLEWE
                     ARCVTDTACVGFRYHALRLLGNARSERYADLIRREVERVFRPGLRGETWGGSAENRAM
                     LKYGTIALARIGRADDRDLILSVVQARPSSDFLEALGYLDDRRSHKILWAAYRSLSRT
                     PTCSSAGLGVPALLPLSRLGDERAISELKDVLRGVGVPPGPWPEHGFPSLCADRAQAF
                     SGLRPRDSTRFAETVIEIAAQEPEGPGTFDAWQALGVMRPEGLGSRVLKLAISRPRWR
                     LVSHYMLKTVVLAVDPELHDEFWNAYQDVQVIPAQLGIRTQVRQGLGYLLFSGVGQWT
                     GD"
     gene            complement(1637..1858)
                     /locus_tag="VLE22_24760"
     CDS             complement(1637..1858)
                     /locus_tag="VLE22_24760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSB17683.1"
                     /translation="MDLQFTAVYRKVPEGFIAFVEELPGANTQGQTLEEARANLEEAV
                     ALVLETNRSLLEEDLKGAEVIREPFTVTR"
     gene            complement(2036..2347)
                     /locus_tag="VLE22_24765"
     CDS             complement(2036..2347)
                     /locus_tag="VLE22_24765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17684.1"
                     /translation="MQTIIAWASRFFIVTKIDGRVERLNLEKTTESHAQERSWRNGDR
                     IRQEFSSERLTAVLDLTITEQCLEEEGCKDGVRYLGDLTVRARETGQQKTIRVEVYCG
                     C"
BASE COUNT          650 a         1005 c          979 g          595 t
ORIGIN      
        1 aacggctggg cgttcagcgg cgagccgagc gagcgcagcg agcggcccga gcgaatgcga
       61 gggcggcgag tccgctgcaa tgccatgttc ggcggccgat gatgaactct caatctcccg
      121 tccactgccc gaccccagag aagagcagat agcccaatcc ttgcctcact tgagtcctga
      181 ttccgagttg cgcggggata acctgaacat cttgatatgc gttccaaaac tcgtcgtgaa
      241 gttccggatc aaccgcgaga acgaccgttt tgagcatgta gtggctcaca agacgccaac
      301 gaggccggct gatcgccagc ttcagaacgc gactacccaa cccttctggt cgcataacgc
      361 ccaaagcctg ccaagcgtcg aatgtgcccg gcccttcagg ttcttgggcg gcgatttcga
      421 taacagtttc ggcgaatcgc gtcgaatcgc gtgggcgaag cccactgaac gcctgcgcgc
      481 gatccgcgca cagggatggg aagccatgct caggccacgg gccaggtgga acaccgacgc
      541 cgcgcagaac gtctttcagc tccgagatgg cacgctcatc tccaagccgg ctcaagggaa
      601 gcagcgcagg cacaccgaga cctgcgctac tgcaagttgg agttcgggag agcgaccggt
      661 atgctgccca gagaatcttg tgggagcgac ggtcgtctag atatcccaga gcttcgagaa
      721 aatctgagct aggcctagcc tgaacgacgg agaggataag gtcccggtcg tcagcgcgac
      781 cgatccgagc cagcgcaatt gttccgtatt tcaacattgc ccgattttcc gcgctgccgc
      841 cccacgtctc cccgcgaagg ccgggtctaa aaacgcgctc gacctctctc cgaatcagat
      901 cggcataccg ctcggacctc gcgttcccca gcaacctcaa agcgtggtat cgaaacccga
      961 cacatgccgt gtcggtcaca cagcgggctt cccattccaa tccccggcta tctccgtaaa
     1021 tcgccagcca ctccgcagct tccttctgca cacgggcatt ccggtccttg agggcttccg
     1081 cgatgatctc gcgcccgctg gtagcgtcac gaatgacgag ctcgcgcata gcggtgattc
     1141 gcttggctgg ttccgcgctc ctatcaagaa cggtagccct agccctggca aagtacggag
     1201 agtcgtagtc gtggatcgga cggctcttgg gaaggtcgcc gggcgaggcc gtgatgccga
     1261 ccagagcgac gagggctccc agcaagaagg tcacgatttg aggtcgcgtg gaactgcttg
     1321 cccgagggcc gatacgccgg tgagataagt actgccgccg aacggtcggg cgttcagcgg
     1381 cgagccgagc gagcgaagcg agcggcccga gcgatagcga gggcggcgag tccgctgcaa
     1441 cgccatgttc ggcgctacgg ttctggaacc tccaaatctc ggcagatctt tcgggcgagg
     1501 aagtcgttga tctcgcggtg ccgcggtacg gtggaggttt tgcgggcgac gcggttcaaa
     1561 aagaccgagt gcctggcacc ctcgcgcctc agctggcacc cgtgtcgttc gagatggcga
     1621 accaggtcgg cacgcttcaa cgagtgaccg tgaagggttc acggataacc tccgcccctt
     1681 tcagatcctc ctcgagcagc gaccggttgg tctcgagcac cagagcaacg gcctcttcca
     1741 gatttgcccg agcctcctcc agcgtctgac cctgcgtgtt cgcgcccgga agctcttcca
     1801 cgaaggcgat aaagccttcg ggcaccttcc ggtaaaccgc ggtgaactga agatccatgg
     1861 ctaggctgat tctaacgccc tgccaattct ccgggcggcg cccaacggtc gggcgttcag
     1921 cggcgcaccg atcaagcgaa gcgagtggcc cgagcgaatg cgagggcggc gaatccgctg
     1981 caacgccatg ctgggcggcg gcatcttttc ttcgaacacg tatgccctcc tcagactagc
     2041 acccgcagta aacctcaact cggatagtct tctgctgacc agtttccctg gcacgaaccg
     2101 tcaaatcccc gagatatctg acgccgtcct tgcatccttc ttcctctaga cattgctccg
     2161 tgatcgtcag gtccagcacc gcagtaagcc gctccgacga aaactcttga cggatccggt
     2221 cgccgttgcg ccaagaccgt tcctgtgcgt gagactctgt ggtcttttcc aggttgagcc
     2281 gctcgacacg tccatcgatc ttcgtgacga tgaaaaaacg ggaggcccag gcaattatcg
     2341 tctgcatgtc agacgtgccc ggcgggaaac ctacacagcc agcgttgttc ggaacgttct
     2401 cggcacccag gaattggatt cgaggtgacg tcggggtgga gagggaagcg gttgggaaga
     2461 acagccctag ggtgacgagg atagcgcaag gcacttggcg attcaacgtg gcgcccaacg
     2521 gtcgggcgct cagcggcgag ccgagcgagc gaagcgagcg gcccgaggga acgcgagggc
     2581 ggcgagtccg ctgcaacgcc atgttcggcg acccggtcat ttcgcgcttc tggcgagttc
     2641 gcgaagtctt ctcagtggca tgtcgccgaa ggcagctact cgctcaccgt ggtaggaata
     2701 cgcagaccgc atccatgcgg gagagccctc catgaagatc acgttgccgt cactgctagc
     2761 atgctcgtgc ctgaaaccga ctcccgcaag ctcgtactca tctccaacag agtagtgtag
     2821 agtggtaccg ggtcggtgct tcaaaatctc cgttacgatc tctctcggtt caccattcac
     2881 cctcctccac tgcgtcagca gaatcgcgct ggcttctttc accatttcct cgggcgaagg
     2941 ctcatcaaga gaagacctcc gcattggctc gcccggaatc ggtccaggtg ttgcctgagc
     3001 ggcgcgaccg atcgaggtgg atgctgcctc tgtagaaaaa ccgagggggc gcatgtacgc
     3061 cgcgacgtac agcaagattt gcgcaagagt gagcgcgcag aggcaccaga cagcgatcgt
     3121 gaggcctctc agcgctttcg cgactgggct gcggtgtcgc gtctcggtca taggtcgccc
     3181 aacggctggg cgttcagcgg cgagccgagc gagcaaagcg agcggcccg
//
LOCUS       DATIFQ010000492         3037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1509012, whole genome shotgun sequence.
ACCESSION   DATIFQ010000492 DATIFQ010000000
VERSION     DATIFQ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509012"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VLE22_24770"
     CDS             <1..546
                     /locus_tag="VLE22_24770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSB17685.1"
                     /translation="GHSTGGGEVTRYIGRHGSKRVAKVVLVAAVPPIMLKTPANPEGL
                     PMEVFDGLRAGVLKDRSQFYRDLAIQFYGANRPGAKVSQGILDQFWLWSMQAGLKNAY
                     ESIKAFSETDFTEDLKKIDVPALVLHGEDDQIVPVKDSAKKSAKLIKGAKEIYYPGAP
                     HGLTATHQDRVNSDLLEFLRS"
     gene            840..1748
                     /locus_tag="VLE22_24775"
     CDS             840..1748
                     /locus_tag="VLE22_24775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB17686.1"
                     /translation="MSLDTTSHADRPGPDELIRTGYALRVGEINALVVSDGTMSIPAS
                     VLAVNAPPSDLGAWLDGMFLPPRFGWPLNVGVVRSGGRTILIDAGLGAEYPDFPGAGQ
                     LTRRLEAAGIGLESLTDVVLTHMHMDHIGGLLGLRGRLRPDLRVHVSAAEAEFWAGGA
                     DLSRATMPPGVPDALRSVAKRFLDEYHSRLQPFEKEYEVAPGVLVRRTGGHTPGHSVV
                     RLESGGDRLMFGGDSVFPPGFDCPHWHNGFDTEPEETVRVRVGLLRELAATGQPLVPT
                     HVSFPAAGRVAVTRDVFRWVPAYWAH"
     gene            2026..2781
                     /locus_tag="VLE22_24780"
     CDS             2026..2781
                     /locus_tag="VLE22_24780"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB17687.1"
                     /translation="MKIVVIGGTGLIGTKTVAILRQGGHEAVAASPNTGVNTITGEGL
                     AKALAGAQVVVDLANAPSWEDKAVLEFFQTSGRNLLAAEAAAGVGHHVALSIVGTDRT
                     PDNGYFRAKVAQEKLIETSGIPYTIIRSTQFMEFLSGIAASAADGSTVRLSPGLFQPI
                     AADDVAAIVADVALAAPRNGIVEIAGPERAPFNEIVARYLKAVGDPREVVSDPEARYF
                     GGRVEERSLVPLGEARLGRIGFDEWLRRSVAQR"
BASE COUNT          573 a          929 c          981 g          554 t
ORIGIN      
        1 ggtcactcaa cgggaggcgg cgaggtcacg cgatatatcg gtcgtcatgg atcgaagcgt
       61 gtcgccaaag tggttcttgt cgccgcagtg ccgccgatca tgttaaaaac tccggcgaat
      121 ccggaggggc tccccatgga agtcttcgat ggtttacgcg caggcgtgct caaggatcgt
      181 tctcaattct acagagattt ggcaattcag ttctatggcg ctaaccggcc cggcgccaaa
      241 gtctcgcagg gcatcctaga tcaattctgg ctttggagca tgcaagcggg cctcaagaac
      301 gcgtatgaat cgatcaaggc attctccgag accgacttca ctgaggacct caagaagatc
      361 gatgttcccg cgttggtgtt gcacggcgag gatgaccaga tcgttcctgt caaggattcg
      421 gcaaaaaagt cggcaaagct tatcaagggc gcaaaggaaa tttattatcc cggagcgccg
      481 cacggactaa cggctaccca tcaggatcgg gtcaactccg accttctgga atttctccgg
      541 tcttgagagg agatccggtg gaacgatgaa ctcgtccggc gaggtctatc agcacgcaag
      601 gatccgcgcg aggttgtcgc tgacccgcac gcgcgctact cctgcgtgaa gctaagcgag
      661 ggcacactcg ttcctggcga tgacgcacga ctcggcgaga cgcgtttcaa aacctggctc
      721 agttagccca caggttaaat tccgagtgcg aatccgcaac tactaacttg gtgaaggaga
      781 atcccatgaa caatatcacg aagtcccttg gagtagggcg ctcagaagag gagacaaaca
      841 tgagcttgga caccacctca cacgccgata gaccggggcc cgacgagttg attcggacag
      901 gctacgcgct gcgggtaggc gagatcaacg cgctggtggt cagcgatggg acgatgtcga
      961 taccagcctc ggtgttggcc gtcaatgccc ccccgtccga cctcggggcc tggctggacg
     1021 gcatgtttct accgccgagg ttcgggtggc cgctaaacgt gggcgtggtg cgtagcggcg
     1081 gccggaccat actcatcgac gctgggctag gggcggagta cccggacttc ccaggggcgg
     1141 ggcagttgac ccggcgactg gaggccgccg gcatcggtct cgaatccctg accgacgtgg
     1201 tgctcaccca catgcacatg gaccacattg gcgggctgct cggcttgagg ggtcggctgc
     1261 gtccggacct gcgggtccac gtgtcggccg ccgaggccga gttctgggcg gggggagccg
     1321 atttgtcccg cgccaccatg ccaccgggcg tgccggacgc gcttcggtcg gtcgccaagc
     1381 ggttcttgga cgagtaccac agccggctgc agccgttcga gaaggaatac gaggtggcgc
     1441 cgggggtgct cgtccgtcgc accggcggcc acacccccgg gcacagcgtg gtccgcctag
     1501 agtctggcgg cgaccggctg atgttcggcg gcgactccgt gttcccgccc gggttcgact
     1561 gcccccactg gcacaacggc ttcgacaccg agcccgagga gacggtccgc gtccgggtcg
     1621 gtctcttgcg ggagctggcg gcgaccggcc agccgctggt gcccactcac gtgtcgttcc
     1681 cggccgccgg ccgggtggcg gtgacgcgcg acgtctttcg ttgggtaccg gcctactggg
     1741 cgcactaacc gctcgttggg cagggccgaa ctaagcgccg caccagaccg gggtcgaaac
     1801 gccggccccg gcaggtgagc tgtgtcgttc ggccccccac atccgctggc gaggttcgtc
     1861 gtgttcctcg tcaaggacaa gggcgctccg gtgctagtgc cagtgaagtg agaggagatc
     1921 cggtggaacc gcggattttc gacgagcgta gatgaaatgg aatccgagta aaggtgcact
     1981 tgaacgctcg tccgaatcac gggcgagcta cagaaaggag tgcatatgaa aatcgtagtt
     2041 atcggcggca ccggcctgat cggaacgaag accgtcgcca ttctgcgcca gggcggccat
     2101 gaggcggtcg ccgcctcgcc caataccggc gtcaacacca tcaccggtga ggggttggcg
     2161 aaagcgcttg ccggcgctca ggttgtcgtc gacttggcga acgcgccttc ctgggaggac
     2221 aaagcggtgc tggaattctt ccagacctcc ggccgcaatc ttctcgcggc agaggccgca
     2281 gccggcgttg ggcaccatgt cgcgctctcc atcgtcggaa ccgaccggac gcccgacaac
     2341 ggctatttcc gcgccaaggt cgcccaagag aaactgatcg agacctccgg catcccctac
     2401 accatcatcc gctcgaccca gttcatggaa ttcctcagcg gcatcgccgc ttcagctgcg
     2461 gatggaagca cggtcaggct ttcgcccggc ctgttccagc ccatcgctgc ggacgacgtt
     2521 gctgccatcg tcgccgatgt ggcgctcgcg gcgccgcgaa acggcatcgt cgagatcgcc
     2581 ggcccggaac gagcaccgtt caacgaaatc gtcgcccgct atctgaaggc ggtcggcgac
     2641 ccgcgtgagg tcgtgagtga ccccgaggcc cgatacttcg gcggccgggt cgaggagcgc
     2701 tcgctcgtgc cgttgggcga agcgcgcctc ggccgcatcg gtttcgacga atggctccgc
     2761 cgctccgtag ctcaaaggta agttagccag gcagggtcga cggcagctcc gagtagagcg
     2821 ccgaaccgta aggagttgcc cgtcactctg ccaacacttt accgcggagt tcgccttcgc
     2881 atccgcgaag cggccgactg tcagaacgga tgggtcggtt cggcatcttt tctctcgtcc
     2941 cggaagcttg aaacttttaa gtgcaagttg ggtgatgatg tgaactgccc cgagtgccgg
     3001 actcaaaatt cagcggatgc cgtcttctgc gagaatt
//
LOCUS       DATIFQ010000493         4610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2467756, whole genome shotgun sequence.
ACCESSION   DATIFQ010000493 DATIFQ010000000
VERSION     DATIFQ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467756"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VLE22_24785"
     CDS             complement(<1..605)
                     /locus_tag="VLE22_24785"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17688.1"
                     /translation="MKALGAGIIGTGWVSDEYVKAFQKNPHCEVVALCSRDAARAKAK
                     AAEHRLQNCAAFADPAEMLKASGLDIVVVCTPHHLHVEQGVAAARAGKHVIVEKPIAI
                     SVPSLRQLDKEIRSAGVKSIVSFVLRWNPMFDNIRAILDQGLIGKVFYAEVDYLHGVG
                     PWYQLWSWMAKKEYGGSSLLAGGSHAVDGLRWFVRDEAVEVT"
     gene            complement(649..2055)
                     /locus_tag="VLE22_24790"
     CDS             complement(649..2055)
                     /locus_tag="VLE22_24790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17689.1"
                     /translation="MHRKAILLLLAWLAAAVDGTAAGSSVTIDVSQRFQTIAGFGVNF
                     NGTYFRDAQKPMIDMLIDDLGATIFRLDPYGIINWETQNDNDDPGVMNWEYYNDRYSI
                     PTFEASWASARYLNSRGIRPFLTLSGRPPDWMLDTKLPPLPGRKPSTGPDHLNPGMYE
                     EFAETLVSMLVYARTKARIDFEYFSPLNETDCPPNEGPRIDPEEMPKVLESIARRMKK
                     EGLGDVKLVVCDQCRLGNDYFGPILQNEELMKQVAVFSLHSYGQQPITPHVDRVRNSK
                     SPQLPLWLTEYGDLNDLDKSQDNEWKNFSLAATKRALRALNEGASVALFWDAYDNYHE
                     HYPRLTFYGLVQNTDHIYAPKKRYFAAKQLYHFVRPGSQRIALNSDAAGLLLSAFRNA
                     ADGTLTVVGVKEGGPTEFDLVLAGTGARVRAWDVYLTTRDVDCRKVDTVAVKDGGAKI
                     SLPPDAIFTLVATVKESD"
     gene            complement(2132..>4610)
                     /locus_tag="VLE22_24795"
     CDS             complement(2132..>4610)
                     /locus_tag="VLE22_24795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17690.1"
                     /translation="YYHRNSALGARNFYEGEKARIIYHTFNIAGGGPIIRNKTFFYAL
                     WNGERVPGKTFYLRNVPTERMRNGDFSRLSAIVKDPTNGLPFSDNVIPSTRISNVAKV
                     VMDELLPAPNRGDPESPVNNFSWLFPYPDDQFYADVFSIRVDHRLSDKNSLYGRVQMY
                     LPKYVLAGAYPALNWTRLRKSHSWVFTDTHVVSPTVVNTFTFGGNRDAIKDNEVVDGW
                     QPASGADLVQKMGLQGVNNKGIDSPGGSPVFNISGFSTISIRQGGFNLPGLNFNVSDS
                     VTWARGKHVLKIGGELRTYSDYNEQVPNDNFGNFRFNGSMAGHAYADFLLGIPQRSIR
                     LDPLVNRKRTSKELGLFVTDTFKVTPKLTLDYGLRWDYFSATTYDDGLMLNWDPATGN
                     VIVPQAALGSISPLYPKNISIVAGEVVPDPDKRNFAPRLGVAYRLSEKTVIRGGYGIF
                     NEFLGRLGRVSGGGPFQIGETYVNSLAGGSPLFQMPNPFPASLVTATIPSQSVTAYPM
                     ETKNGLIHQFNISLERQIADMGFRLSYIGSRNRDMNYSIGTNKPQPSLIQFDDSRRPY
                     PQFVGTTVYRTNGAANYDSMSFAANRRVGWVTFDVHWTWAHGMNNMLNYGTDTQLDLE
                     NPYAPLLWNRDFMAKHRAVFNTLWDLPFGRGKRYLNSVPAAANHIIGGWSIAWVAYLQ
                     GGQYFSPSFSDADPSNTDTFGGFPDRICNGNLPTGQRSIDRWFDPGCFVNPPAGRFGN
                     SGANILEGPGLHAHNVTVVKNFKITERVTFDFMTLISNIFNHPNFLFPAADISTEGVG
                     VIGETHGLYSGERAGQRMIELRGRIRF"
BASE COUNT          914 a         1326 c         1395 g          975 t
ORIGIN      
        1 gtaacctcga cggcctcgtc gcgcacgaac cagcggagtc cgtcaaccgc gtggctgccg
       61 cctgccagca gggaactgcc gccgtattct ttcttggcca tccaggacca cagttggtac
      121 caaggcccga ctccgtgcag atagtcgacc tctgcgtaga agaccttgcc aattagcccc
      181 tggtccagga ttgcccgaat gttgtcgaac atcggattcc aacgcaggac aaaactgacg
      241 atgctcttca cgccggctga ccggatctcc ttatcgagct ggcgcaggct cggtacgctg
      301 atcgcgattg gcttctcgac gatgacgtgc ttgccggcgc gggcggcggc aacaccttgc
      361 tcgacgtgca ggtgatgggg cgtacagacg acaacgatgt cgaggcctga cgctttcaac
      421 atctccgccg gatccgcgaa ggcagcgcag ttctggaggc gatgctcggc ggccttcgct
      481 ttcgcccgtg cagcgtcccg gctgcacagc gctaccacct cacagtgagg attcttctga
      541 aatgctttga cgtactcgtc cgaaacccat ccggttccaa tgatgcccgc accaagtgct
      601 ttcatccgtg tcagctcctt ctgggattgt aagtcaactc ttccgatctc agtctgattc
      661 tttcacggtt gcgacaagcg tgaagatggc gtccggcggc aggctgattt tggcgccgcc
      721 atccttcaca gcgaccgtgt cgaccttcct gcaatctaca tccctcgtcg tcaaataaac
      781 gtcccaggcc ctaaccctcg cgccagtccc ggccaggacg agatcgaact cggtggggcc
      841 gccctctttg acgccgacca ccgtcagcgt accgtccgcg gcattgcgaa atgccgacaa
      901 caacaacccc gctgcatcgc tgttcagagc tatcctctgc gaacccggac ggacaaaatg
      961 gtaaagctgc tttgccgcga agtatcgttt cttcggcgca tagatgtgat ccgtgttctg
     1021 cacgagtccg tagaacgtca gccggggata gtgctcatgg tagttgtcgt aggcgtccca
     1081 gaacagcgcc acgcttgcgc cctcgttgag ggcgcgcaac gcccgcttgg tggcggcaag
     1141 cgagaagttc ttccattcgt tgtcctggga cttgtcgaga tcgttcaggt ctccgtattc
     1201 cgtcagccag agggggagtt gcgggctctt gctgttccgg acacggtcca catgaggggt
     1261 gatgggctgc tggccgtagc tgtgtaagga gaagacggcg acctgcttca tcagttcctc
     1321 gttctggagg atcgggccga agtagtcatt ccccaggcgg cactggtcac agaccacgag
     1381 cttgacatcg ccgagtccct ctttcttcat gcggcgcgcg atggattcga ggactttggg
     1441 catctcctcc ggatcgattc gcgggccttc gttaggaggg cagtccgttt cattcaaggg
     1501 gctgaagtac tcaaagtcga tacgagcctt cgtccgggcg taaaccagca tggagaccaa
     1561 cgtctccgca aactcctcgt acatgcccgg attgagatga tcaggcccgg tagagggttt
     1621 gcgtccagga agcggaggca gctttgtgtc aagcatccag tccggaggtc tcccgctcaa
     1681 cgtcagaaag ggacggatgc ctcgcgagtt caggtatcgc gccgaggccc aggacgcctc
     1741 aaacgtggga atggagtagc gatcattgta atactcccaa ttcatcacgc cagggtcgtc
     1801 gttatcattc tgggtctccc agttgatgat cccgtacgga tccagtcgga agatggttgc
     1861 gccgaggtcg tcaatcaaca tgtcgatcat cggtttctga gcgtcgcgga agtacgtgcc
     1921 attgaagttg acgccgaaac cggcaatcgt ctggaagcgt tgtgacacgt caatcgtcac
     1981 gctgctaccg gcggcggtcc catcaactgc tgccgccagc cacgcgagga gtagcaggat
     2041 cgctttgcga tgcactggat gtgggtgtgc caaaccggga agaaggaggg gccgcagccc
     2101 aggaaaggac tgcggcccag atctgtctga ctcagaagcg gatgcggccc ctcaattcga
     2161 tcatccgttg cccggctctc tctccggagt agagcccgtg ggtttcaccg atcacgccga
     2221 cgccctcagt cgaaatgtcg gcggccggga acaggaagtt ggggtgattg aagatgttcg
     2281 agatgagggt catgaagtcg aaggttaccc gttcggtgat cttgaagttc ttcacgaccg
     2341 tcacgttgtg ggcgtggagg cccggtccct cgagaatgtt ggccccgcta ttgccgaagc
     2401 gtcctgccgg aggattcacg aagcaaccag ggtcgaacca ccggtcgatg ctccgctgtc
     2461 cggtcggtag gttgccattg cagatccgat cggggaaccc gccgaaggta tccgtgttcg
     2521 acggatctgc atcggagaac gaaggcgaga agtactggcc accttgcaag taggctaccc
     2581 aggcgatgct ccaaccaccg ataatgtgat tcgctgcggc tggaacactg ttcaggtacc
     2641 gcttgccgcg gccaaagggc aggtcccaga gggtgttgaa cactgcccga tgcttggcca
     2701 tgaagtcccg gttccacaac agcggagcgt agggattctc gagatccagc tgggtgtccg
     2761 tcccgtaatt caacatgttg ttcatcccgt gcgcccaggt ccagtgaacg tcgaaggtca
     2821 cccagcctac gcggcgattg gccgcaaagc tcatggagtc gtagttggcc gccccattgg
     2881 tgcgatagac cgtggtcccg acgaactgcg ggtacggacg gcgggagtcg tcgaactgga
     2941 tcaggctcgg ttgcggcttg ttggtcccga tgctgtagtt catgtcgcgg ttccgcgagc
     3001 cgatgtaaga gaggcggaag cccatgtcgg cgatttgtcg ctccaagctg atgttgaact
     3061 ggtgaatcag cccgttcttc gtttccatcg gataggcggt gacactctgg gatggaatgg
     3121 tggctgtgac gagactggcc gggaacggat tcggcatctg gaacagcgga ctgcctcccg
     3181 cgagtgagtt gacgtatgtc tcgccgatct gaaacggccc gccgcccgaa acacgcccaa
     3241 gacggcccag gaactcgttg aaaatcccgt accctccacg aatgactgtc ttctcactca
     3301 gccggtaagc gacgccgaga cgcggggcga aattgcgttt gtccggatcg ggaaccactt
     3361 cgccggccac aatgctgatg ttcttcgggt agagaggact gatgcttccc agcgccgctt
     3421 ggggcacgat cacgttgccc gtagcagggt cccagttcag catcaggcca tcgtcgtacg
     3481 tggtggcgct aaaatagtcc catcggagcc cgtaatcgag ggtcagcttg ggagtcacct
     3541 tgaacgtatc cgtaacgaac aggccgagtt ctttggacgt ccgtttgcgg ttcaccagcg
     3601 gatctaaccg gatgctccgt tgcgggatac cgagcagaaa gtcggcatac gcatgcccgg
     3661 ccatcgagcc gttgaaccgg aaattgccga aattgtcgtt cggaacctgc tcgttgtagt
     3721 cgctgtaggt cctcagttcg ccaccgatct tgaggacgtg cttgcctctt gcccacgtca
     3781 cggaatcgga gacgttgaag ttgagaccgg gtaggttgaa gcccccctgt cggatcgata
     3841 tcgtgctgaa gccactgatg ttgaatactg gagagccacc cggcgaatcg atgcccttgt
     3901 tgttgacgcc ctggagcccc atcttctgaa ccagatcggc tccggaggcg ggctgccagc
     3961 cgtcgactac ctcgttgtcc ttgatcgcat cgcggtttcc gccaaaagtg aaggtgttca
     4021 caacggtcgg cgagaccacg tgcgtgtcgg tgaagaccca ggagtggctc ttgcgcagcc
     4081 gggtccaatt gagcgcgggg tacgcgcccg ccagcacgta cttcggaagg tacatctgga
     4141 ccctgccata gagagaattc ttgtcagaca ggcggtgatc gacgcgaata gagaacacgt
     4201 ccgcgtagaa ttggtcgtcc gggtacggga acagccaact gaagttgttg accggcgatt
     4261 cgggatcgcc tcggttcgga gccggaagca gctcatccat gacaaccttg gcgacattgc
     4321 tgatccgcgt tgacgggatc acattatcgg aaaacggcag gccgttcgtc ggatctttga
     4381 cgatggcaga gaggcgggag aagtcgccgt tcctcattcg ctcggtcggc acgttgcgca
     4441 ggtagaaggt cttgccggga acacgctctc cattccacag ggcatagaag aaagtcttgt
     4501 tacggatgat cggaccccct cccgcgatgt tgaatgtgtg atagatgatc ctcgccttct
     4561 cgccttcata gaagtttctg gcgcccagcg cggaattgcg gtgatagtag
//
LOCUS       DATIFQ010000494        22319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_553808, whole genome shotgun sequence.
ACCESSION   DATIFQ010000494 DATIFQ010000000
VERSION     DATIFQ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553808"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VLE22_24800"
     CDS             complement(<1..969)
                     /locus_tag="VLE22_24800"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSB17691.1"
                     /translation="MDAVDFLKACDDIKAGRDSRESKFYLWSDSADAPPPGPAGKDTY
                     FMVRPTHLPGDGPGGLYDSSKHEFWQYSERAAKYYWTKWGGVDKGGKRTRLPQYNWGS
                     REFQEEAEKIVRFWMDTGIDGMIIDAVNWYVDYTWELGRRRITDVIASYGNAYSQPEG
                     AGGFHEDPAPWVTEGGWNSVQNYALNIWWEKGSNVIHNAIETGDPRPIERALRDYRDR
                     VVEAGGTLYFAPEARSRIDDASKRRLAAAVVITTGHLVAVHEGPEGLLVDPEVRRLIE
                     AKRAHPALGQLARRRQLATRADDKHYAFLLTAPGNSERMMVAMNFQA"
     gene            complement(1012..2244)
                     /locus_tag="VLE22_24805"
     CDS             complement(1012..2244)
                     /locus_tag="VLE22_24805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17692.1"
                     /translation="MKVLANIRIAFRALRVNRLRSVLTMLGIIIGVAAVIAMVAVGSG
                     ATARIKAQIQSIGSNIIMVLPGSLTTGGIRLGAGANVSLTEDDARAIAAECPAVSAAA
                     PVVRGGAQVMYGNNNWATSIQGTIPDYLTVRDAALSAGRPFTGQDVDAAAKVALLGQT
                     VADNLFGDADPVDQVIRIKNVPFTVVGVLARKGQSPGGQDQDDVILVPISTAKRSIVG
                     SNRANARSVGAIMVQAAAPSLMAEAQQQLEVLLRQRHHMQQNQEDDFTVRNLSEVFDA
                     QESSARVMSLLLGAIASVSLIVGGIGIMNIMLVSVTERTREIGLRLAVGAKTRDILSQ
                     FLVEAVTLTALGGIVGILLGVGASATISYFAQWSTLISPSAILLAFVFSAFVGVSFGY
                     YPARKAAFLDPIDALRYE"
     gene            complement(2241..2999)
                     /locus_tag="VLE22_24810"
     CDS             complement(2241..2999)
                     /locus_tag="VLE22_24810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB17693.1"
                     /translation="MATLIEVRDLVRRYTVGAVVVSALDNVSLEIERGDFVAVMGPSG
                     SGKSTFMNILGCLDKPTSGVYLLDGIDVGGLDRDGLAEIRNRKIGFVFQQFNLLARTS
                     AVENVELPLLYSTQPLAEHRARALSALTSVGLAKWADHQPTQLSGGQQQRVAIARALI
                     NEPQLILADEPTGALDSRTGIEIMSIFQRLNREAGITVVLVTHDPDIAAFGNRNVHFK
                     DGRVVDDLRIARPRRADEELERLPAGEEKELVFT"
     gene            complement(3002..4411)
                     /locus_tag="VLE22_24815"
     CDS             complement(3002..4411)
                     /locus_tag="VLE22_24815"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB17694.1"
                     /translation="MRAPNRKVFLVTVAAVAVAVGGSWTVLRGHGDTQYRTVSLDKGD
                     VAYSVSATGSPNAVVTVQVGTQVSGNILELDADYNTRVKKGQLVARIDPQAFQARVTQ
                     ASANVDVAKAAVVNARAVVERAQAEVASAKAGVADAAASVLKAKVSVQDAKNKLDRAV
                     LLRQAGVIADQDKDTAQAAYDTAVAMQQASEAQQAAAVQKVRSAEADIEVSRTQLATA
                     GAQVKQTTAALEQAQLDLSHTYITAPVDGVVVARRVDVGQTVAASLQAPTLFEIAQDL
                     TKMQVDTNVSEADVGRVRVGQPATFTVDAYPGEVFRGQVTQIRQAPINVQNVVTYDVV
                     IGVANTDLKLFPGMTANVKILVDRHADVLRAPNAALRFRPVGSEKQRAAAGSAGPSSR
                     STGRRGAANMSTLWVPAGKGEPRPVVVQTGISDGVYTEITSGGLKPGDQVIVAEAGTT
                     AAQTPVSPAGPGGRRGMGF"
     gene            complement(4530..7811)
                     /locus_tag="VLE22_24820"
     CDS             complement(4530..7811)
                     /locus_tag="VLE22_24820"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB17695.1"
                     /translation="MLRRLETLTLLLGTIIATSSAQTTTGTITGTITDPTGAVMTGAK
                     ITATNTQTGVRTTTVSNASGAYVLPFLAIGRYSVTVEARGFKTANIQPFGLEVAQTAR
                     VDIRMQVGEISQSVDVTDVAPVLQTDTTHTGDTISSAKSSTLPLNGRNFVALSLLIPG
                     AVTPNPTSFTTPSRAFSGGRPYVNGNREQTNNFLLEGIDVDDPIDNLVGYNPNVDAIG
                     EMKVITGNAPAEFGNSNGAVVNVALKSGTNALHGDLFEFLRNDRLDANSFFSNRSGAR
                     KRALRMNTFGGTLGGPIVQDRTFFFIDYQGTRNPTSGAALASVALAPYRVGDLSAFSQ
                     TIRDPVTNLPFPGNVIPRDRIANPVAKALFSNPSLYPLPNATGTGPIGVTNNYISSSG
                     NFLDNDQADAKIDTQLTASDRLSGRFTIARYRSGDTTVGLPVFLGGGTNAPTTGGVLN
                     WTRTLNPQTVNEARFGFNRVVLKFFTTDPTGVLGANGNQTLGIPGSQPIAGASSVSLG
                     DGLSSIGAAATDSSTYDNTYQFSDNFIWQRRLHTFKAGFQIIRYQQNRFYAGNNGLLG
                     TFSYTGDYTGSAFADFLLDDLRSKGRGSQTGLWGQRQSRIAAFFQDDFKLRPDLVLNL
                     GLRWEYAQPLIEVADRQSNIDLVTGQQLFAGKDGNSRALYDPYYRQFMPRVGVAWTPP
                     MLSNKFVARAGYGITSFMEGTGANLRLPLNPPFFFESNSTYDLASPGSITSGFTDVQP
                     QNQLSGQVRAWIPNLRPAFIQQWNVSGQYEFTKTLSFTAGYVGQRGTHLVDPREFNQP
                     LPGTGPVDTWLPLQQRRPLYAFDPLITNISGTDSSSTMSYRALQLSARKRLASGLEFL
                     SAYTYSRSLTDNLGYYGSAGVASEGAYWQNAYDRKADWGPAFFDARHVFSLGGYYELP
                     FGRDKRWGQHTSKPGNLILGNWAMSYVASLHSGFPITILSSDVSNQAVRGSTRPDQFL
                     PLTYTGQTIDQWFGTGNSFCLTPGVDDGACAYGVPALGTFGNAGVGTERAPAFKNLDF
                     ALDKKFSLSESHALDFRAEFFNILNHSNFGPPGRSISSPATFGVITSTIGLPRNIQFG
                     LKYTF"
     gene            complement(7925..8677)
                     /locus_tag="VLE22_24825"
     CDS             complement(7925..8677)
                     /locus_tag="VLE22_24825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB17696.1"
                     /translation="MRWKIAEISRTEHDTRALAGDNRPPRILVVDDERAIRTLLNVSL
                     SAHGYAVYQAEDGEQSIQQVRSVRPDVILLDLGLPDVDGTEIIRCVREWAKTPIIVLS
                     VRDDETQKIAALDAGADDYLTKPFGPGELLARIRAALRRACVVGDSPTFVCGDLLVDL
                     MRREVRLSDEPVQLTPTEYDLLAVLVHHAGKVLTHRQLIHEVWGGAYYHDASHLLRVT
                     ISNLRRKLERDTARPRYIVTEPGVGYRLRSES"
     gene            complement(8688..9887)
                     /locus_tag="VLE22_24830"
     CDS             complement(8688..9887)
                     /locus_tag="VLE22_24830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HSB17697.1"
                     /translation="MHDPAAPAAPEIALGVVHGNVRRRPWFSYLVSLSLVLAITLTGL
                     QIEHQMTATNLAMVYLLAVVVTALQWGLLPAVVTAVTSTIVFAYFFVPPYRSFAFRDD
                     WYLITFVTFLVAGLIISRLASEATQNARRAAACAIERARLQEKAQEAHWVEERNRLHK
                     ALMNSVSHNLRTPLASINGALNSLIEDGPALSESVRNELLESALEQSVALNRLVGNLL
                     DVTRLEAGALRLRIEPCEMQDLIGSALAQLGSAISSRRISVDIPPDLPLVYMDFVLVS
                     QVLVNLVDNALRYSPVDRPVEICARIEGGHLRVGVADRGIGIESGDLMRIFEKFQRAG
                     NAANSNGIGLGLYICKGFVEAHSGRIWAERRVQGGTLITFEIPRLGEFGFLPVPYSGA
                     PQELTAA"
     gene            10136..11716
                     /locus_tag="VLE22_24835"
     CDS             10136..11716
                     /locus_tag="VLE22_24835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB17698.1"
                     /translation="MTRFCGIASAAIIAGVVLFLIPCRAETIRVSNSYGGISVFVVTG
                     SQIRVRQSSSVRPVHADDVQITKAPGLIAVDCQPADGAQVDIGIGLPYGAAIEATTKN
                     GAVSLEGLIARAFVRTVGGDILLTAPWEATRLTISSRAAPRGFAAPGGIKLSQRTQDG
                     AWLLEDKLPESKVTYGVIRVEAESAGRIVLSEMPIPADSPVKMHWQAPEILDGIMNGG
                     LRNRASSSRAPINPPAPAAAAEAGTPVFTSDVRMVNVTVAVFDGEGHHVDGLKPGDFQ
                     ILEDGVPQKPAIAGSEEVPFNLAILLDLSGSTLRDRPAMKEAARRFIGIARPHDRVAV
                     YALANDSFHVVSRLTADREPLLAAIEAIPEVSGGSPLYDTVVLAYAEEFLEHPKERNA
                     LIFISDGVDNQIHGVSTPSKVSFRKLQRAAAEMTALIYPVYLDPFTVAPPPRWAVSAR
                     SALGKLAEATGGRLFRASSIQDLDPVYPQVAEELRSVYTVAYYPANQDFDGKWRRVEV
                     RVNRPGVKVRTRAGYFAK"
     gene            11805..13901
                     /locus_tag="VLE22_24840"
     CDS             11805..13901
                     /locus_tag="VLE22_24840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17699.1"
                     /translation="MVRILSISFVLRLILCAPAHASTPVLPDLHAPGGGPIRVEVKKG
                     ARTIVTTDYWRLEFDLRRGGVLDSIVFLHGSGRNVLVRPFRTHVGDWSDQDAGMAEVA
                     SSQDRDVVRLEFRGTMGAPGDRKGPVSFTTTWTLSPFAVRAEHAVRLAEDVSVRSAGI
                     GSMSVRAELNEFSWRPGVATDSRRMHLARSGRAAGEGAQAITDYNTPLWLLVFHRAVE
                     GFDLTPGSDYETWQGRLGADAGGRLYEVKAASDGSSVEILREPLHSKEPVTARKGEYK
                     FAYYVGLPRIVERSNRKWRHLSLGNRPFPSDDEIRRWAENGVNVVRYHNDFSPDGNFW
                     HDGAWPPYDEKGMIELRRVIAACKRNKIAIVPYFSVYEFHPEAQGFDRYAGEWMRMGD
                     PGGAMIHNLVCANNEGEYGAQMCPNSGWLERRKQDIERAYRDLGFDGIYYDWVKTLSC
                     FNPKHGSAHHLSTDQVVDLMAWTRRLVRPNGIVVLHIYGHTASLALENFADLVVNMEE
                     ASATERWMKITDVPIITRLAESIPRSPCPSYRPDHTVDRNRNNISQLVVLGMFPAWRA
                     TGPSVAPPGGPGYELTLKLFRDFKPYRLEAYRFHDAFSGAVKTASDDVYGAAYGSAEP
                     PLVVISNTSQEKRSGVSWRVDASKLGFGTPARVTLKDTRSGETRTLPVGALSDGSLAI
                     DLDGWEYGIFEIRAAK"
     gene            13922..15223
                     /locus_tag="VLE22_24845"
     CDS             13922..15223
                     /locus_tag="VLE22_24845"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB17700.1"
                     /translation="MRNPISRRALLAGLAAPRAARPPSFLIMLSDDQGYGDAGCYGGE
                     IPTPNIDSIARNGVRFTQYYAAGPVCTPSRYGLLTGRYPARSRDNLLGALMPGSKKGI
                     HSGETTVADVLKGRGYRTAVFGKWHLGDALPEFFPTRHGFQRFDGFVHGCIDFFDFTY
                     GGMKCWYRNESLFQPPRGYTTDYITDRAIEFLRDLRNRPFLLYLPYNAPHYGKTWYDP
                     VSRKARNALQAPEDYVARFAHIRDKDRQGYAAMVASLDDNIGRLLNAVRELKLEEDTL
                     VVFASDNGGSFPYGGRNGPLRGQKGELFEGGIRVPCAMQWKGRIAPGSVTSQPAGGVD
                     LMPTLCALAGSAVPKQVDGRDLRPVLFERRSLERELFWRTGDADAYRSGDWKYIRIAG
                     GRELLFHIESDPGEKVDRSSDTARLAQIRERYRRLLASGLE"
     gene            15259..15876
                     /locus_tag="VLE22_24850"
     CDS             15259..15876
                     /locus_tag="VLE22_24850"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HSB17701.1"
                     /translation="MKTSAKLDLYKEFEKQYVKPTKPIFVNIPAIPYLTTDGSGKPGS
                     EDFQRKIGALFGVAYTIKMARKSAGRDYKVCGLEGLWWFDDANKPVSELPLDEWNWKL
                     MIRVPDFVTAREVRQAVTALAKRGKLPDGVEVRLEKIKEGQCVQMLHVGPYQEETRTV
                     QAMKEFAEANKMRFHGPHHELYISDPNRVASERLRTILRQAVRLA"
     gene            complement(15878..16870)
                     /locus_tag="VLE22_24855"
     CDS             complement(15878..16870)
                     /locus_tag="VLE22_24855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17702.1"
                     /translation="MRAGTILLLLTLAAGAADRTLELRGQIEPAPGKASVTIDATFGS
                     FTASTISDARGRFKFKKLEPGPYSVTVFIAGAGVSRRTVEVTPSLADPEGRVHAVIPF
                     NPTPVSAGRLAARRNTVSVGDLSIPDRAKREYDEAQQKLGGNDVEAAVRHLERAVEIA
                     PGYMMAWNNLGTIAYHARKFEEAETYFRKALEARPGAFPPAVNLGGVLLYTGKHEEAL
                     KFNKYAVETRPRDALANSQLGMTYFLLKQDDEALKYLNAARELDPSHFSNPQLLMAEI
                     YIRRRDRVLAIKELKEFLALHPDSSEAPKVRGFLDRLMNPPRPATQAATPTPAP"
     gene            16919..17305
                     /locus_tag="VLE22_24860"
     CDS             16919..17305
                     /locus_tag="VLE22_24860"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HSB17703.1"
                     /translation="MSGYEILAATFLALHLLWIVWVIAGVVVTRGRPALVAGHVASLL
                     YGIAIETGPWPCPLTLAEQWAREKAGITPYREGFIVHYLEALVYPDVPQEWLTRGAVA
                     VCLALLGVYGRRWWRWTRGRVVARRE"
     gene            17446..18471
                     /gene="tdh"
                     /locus_tag="VLE22_24865"
     CDS             17446..18471
                     /gene="tdh"
                     /locus_tag="VLE22_24865"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HSB17704.1"
                     /translation="MKALVKSKAEPGLWLEDVPMPEIGINDVLIKVKRASICGTDVHI
                     YNWDDWAKKTIPVPMVVGHEFVGEIVKTGANVSDFFPGMLVSAEGHVVCGRCRNCLAG
                     RRHLCNKTLGVGVNRPGAFAEYISVPMTNVWHHPDSMDLEVASIFDPFGNAVHTALSF
                     PVLGEDVLVTGAGPIGIMGAAVARHAGARYVVITDVNPWRLELAKKLGVTRAVNVKTT
                     RLKDVQKELGMKEGFDVGFEMSGNGSAFREMLENMCHGGRIAMLGIPGGEMAIDWNTV
                     IFNMLTIKGIYGREMYETWYMMNVMIESGLDIKPVITHRFHYTEYEKGFDAMRSGESG
                     KVILNWE"
     gene            complement(18452..20002)
                     /locus_tag="VLE22_24870"
     CDS             complement(18452..20002)
                     /locus_tag="VLE22_24870"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HSB17705.1"
                     /translation="MKKVAILTALEAVRLIPDDATVATSGFVGCGHPEELTTALERRF
                     QLEGSPRGLTLVYAAGQGDGKSRGLNHIAHKGLLRRVIGGHWNLAPGLGRLALEDEIE
                     AYNFPQGVMSHLFRAIAAGNPGLVTHIGLDTFADPRHGGGKLNARSTEDLVEVVRLGE
                     KEWLWYKAFPIDVALLRGTAADSYGNISLDDEVLTGEALSIALSAKNSGGAVVVQVER
                     IVPDFSRDPKSIRIPGILVDAVVMGSQENHMQTFAEQFNPAYVEPGDICAGGQSPALN
                     GVRLWIARRAFAEIRDGDVVNLGIGIPEGVAQVAATQDRLRSMVLTVEAGAIGGIPAG
                     GLSFGASSHPMAIIDQPYMFDFYDGGGLDIAVLSMAECDRCGSVNVSRFAGRIAGAGG
                     FTNISQTAKRVVFVGTFSAGGLDVRWEGGRLQVRAEGETRKFVRDVGHVTFSGPRATA
                     HGREILYVTERAVFQLAAGGLELIEVAPGIDLRRDVLSQMEFTPSVAAELKPMPAWIF
                     DPLTPSSE"
     gene            20103..21377
                     /locus_tag="VLE22_24875"
     CDS             20103..21377
                     /locus_tag="VLE22_24875"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17706.1"
                     /translation="MENKEIDRRTFLGAAAGSFMIMKPELVRGSQRNSAVRMALFGCG
                     GRGTGVAEGFTLETTAQYVALGDLYQEQTERAQATINKAAEKKGKTPINSAMLFTGPK
                     ALDKLTAMRELDVIHIATPPYFHPAHFEQAAASGKHIYLEKPVAVDVPGAKKVMRIGS
                     QIGKKASVAIGFQLRHATPYVQLVERIRRGDVGDIVCGLCHYYAGAIPQPDRPGAGPA
                     TKRLRNWLHDRVLSGDILVEQNIHLIDTTNWVLKAHPVMAQGVRGAKGRRDAGDCSSH
                     FNVNYTYPGDIHITLTSTQFIEGSWDVAMRYFGTGGNAEMRYDSPVRIDGPKKWDFPI
                     GQKAGEITDVGAAAAGAFKGALDDADTMKQRYVIESIVSGNHINEAEQGGISTLSAIL
                     GRQAAYTGRNWSWDELLKVEEVWDAKIDVAKV"
     gene            21477..>22319
                     /locus_tag="VLE22_24880"
     CDS             21477..>22319
                     /locus_tag="VLE22_24880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB17707.1"
                     /translation="MKRRSFFGSVFAGLGSAATARPAKAKVGDIPVRPLGRTGQKLTI
                     VGMGGARFHLSGWEDGTVLVRRAYDLGINYFDMARSYWNGRAEEVYGAVLPEFRKNVF
                     ITSKSNRRARKEAEAELDLSLKLMKTDYLDLWQIHGVGTDEEVSRILGPDGALEAFTA
                     AKKAGKCRFIGFTGHSDPNVNLKMLRGFDGFDTMLMPLHVADTFYLSFEKLALPAAIE
                     HGIGMLGMKIFGNAFLLRAFSVPECLQYTLSLPVSAVTLGFSTQGQLEDDVRIAQNFK
                     PLPAE"
BASE COUNT         4326 a         6533 c         7259 g         4201 t
ORIGIN      
        1 tgcctgaaag ttcatcgcta ccatcatgcg ctcggagttg cccggagccg tgagcaggaa
       61 cgcgtagtgc ttgtcgtcgg cgcgcgtcgc aagctgccgc cggcgtgcga gctgccccag
      121 agccggatgc gcgcgctttg cttcgatcag ccggcgcacc tcgggatcca ccagcagtcc
      181 ctccggtcct tcatgaaccg ctaccaggtg gcctgtcgtg atcaccaccg ccgccgccag
      241 cctccgcttg ctggcatcgt cgatgcggct gcgcgcctcg ggcgcgaaat acaaggtccc
      301 acccgcctcg acgacgcggt cgcggtagtc gcgcaacgcc cgttcgatgg gacgcgggtc
      361 gcccgtctcg atagcattgt ggattacgtt ggagcctttc tcccaccaga tgttcagcgc
      421 gtagttctgc acggagttcc agccgccttc cgtgacccag ggcgccggat cctcgtgaaa
      481 gccgcccgcg ccttcaggct gagagtacgc gttcccgtaa ctcgcgatca cgtccgtgat
      541 gcgccggcgg ccgagttccc aggtgtaatc cacgtaccag ttcaccgcgt cgatgatcat
      601 gccatcgatg ccggtgtcca tccagaaccg aacgattttc tcggcctctt cctggaactc
      661 gcggctcccc cagttgtact gtggcaggcg ggtccgtttg ccgcccttgt caacgccgcc
      721 ccactttgtc cagtagtact tggcggctcg ctcgctgtac tgccagaatt cgtgcttgct
      781 ggagtcgtac aggccacctg gcccgtcacc cggcaaatgg gtcgggcgga ccataaagta
      841 tgtgtccttg ccggcaggac ccggaggcgg cgcgtccgcg ctgtcactcc aaagatagaa
      901 ttttgactcc cggctgtctc tgcccgcttt gatgtcgtcg caggccttca ggaaatcgac
      961 ggcatccacg ctcgaggacg ctctgcgccc gagcgcgccg ggcggccgtg gtcactcgta
     1021 gcgcagagcg tcaatgggat ccagaaaggc tgccttgcgg gcgggatagt agccgaacga
     1081 gacgccgaca aaggccgaga acacaaaggc cagcagaatc gcgctcgggc tgatcagagt
     1141 cgaccactgg gcaaaatacg agatcgtggc gctggcgccc acaccgagca ggatgcccac
     1201 gatcccgccc agcgccgtca gcgtgaccgc ctccaccaga aactgggaca ggatgtcgcg
     1261 cgtcttggct cccaccgcaa gccgcaaccc gatctcgcga gtgcgctcgg tgaccgagac
     1321 gagcatgatg ttcataatgc cgatcccgcc gacaatcagc gataccgatg cgatcgcgcc
     1381 caacagcagc gacatcacgc gggcggagct ttcctgcgca tcgaacacct cggacagatt
     1441 gcggacggtg aaatcgtctt cctggttttg ttgcatgtgg tggcgctgcc gcaggaggac
     1501 ctcgagctgc tgttgcgcct cggccatcag gctcggagcg gcggcctgca ccatgatggc
     1561 gccgacagag cgtgcgttgg cgcggttgct tccgacaatg ctccgcttag ccgtcgagat
     1621 cgggaccaga atcacgtcgt cctgatcttg cccgcccgga gactggcctt tccgggcgag
     1681 gacaccaacg acagtgaaag gcacattctt gatgcgaatc acctgatcga ccgggtcggc
     1741 gtcgccaaac aggttgtcgg ccactgtctg tcccagaagc gcaaccttgg ccgcagcgtc
     1801 aacgtcttgg ccggtgaagg gccgaccggc gctgagcgct gcgtcgcgaa cggtcagata
     1861 gtccgggatg gtgccctgaa tcgacgtggc ccagttgttg ttcccgtaca tgacctgagc
     1921 cccgcctcgg accacggggg ccgccgctga aaccgccgga cactccgccg cgattgccct
     1981 ggcgtcatcc tcggtcaggc ttacattagc cccggctccc agccgtatgc cacccgtggt
     2041 caggctgccg gggaggacca tgatgatgtt gctgccgatg ctctggatct gcgccttaat
     2101 cctggcggtg gcgccgctgc cgacggcgac catcgcgatc accgccgcaa cgccgatgat
     2161 gatgcccagc atcgtcagca cggagcgcaa acggttgacg cggagtgcgc gaaaggcgat
     2221 gcggatgttt gccaggacct tcatgtgaac accaactcct tttcctcgcc cgctggcaac
     2281 ctctcgagtt cttcatccgc ccggcgtgga cgggcgatcc gcaagtcgtc caccaccctg
     2341 ccgtccttga agtgaacgtt gcggttgcca aaagcggcga tatcggggtc gtgcgtcacc
     2401 aacacgaccg taatgccggc ctctcggttc agccgctgga aaatggacat gatctcaatg
     2461 cccgtccggg aatccagggc gccggtcggc tcgtcggcga gtattaactg cggttcgttg
     2521 atcaaggcgc gcgcgatggc cactctctgt tgctgcccgc ccgaaagctg agtcggttgg
     2581 tgatccgccc actttgccag tccgaccgaa gtgagtgcgc tcagagcccg cgcgcgatgc
     2641 tctgccagcg gctgagtgga atagagcagg ggcaattcga cattctccac ggcgctcgtg
     2701 cgcgccaaaa ggttgaactg ctggaagacg aaaccgattt tccggttgcg tatttcggcc
     2761 agaccgtcgc gatccagtcc gccgacatcg ataccgtcca gcaagtagac gccggaggta
     2821 ggcttgtcga gacatccgag gatgttcatg aacgtggact tcccgctccc ggagggcccc
     2881 ataacggcca caaaatcgcc gcgttcgatc tccaaggaga cgttgtccaa ggcagatacc
     2941 acgacggcgc ctacggtata gcgccggacc aggtcgcgga cttcaatcag cgtggccatg
     3001 gtcagaaccc cattccgcga cgtccgccag gaccagcggg agagaccggc gtctgcgctg
     3061 ccgtcgtgcc ggcctccgcc acgataacct ggtcccccgg cttgaggccg ccgctggtga
     3121 tctccgtata gacgccgtcg ctgatccccg tttgcaccac cacaggccgc ggttcaccct
     3181 tgcctgccgg gacccagaga gttgacatgt tggccgctcc gcgccgccca gtagacctcg
     3241 acgaggggcc ggcggaaccg gccgcggcac gttgcttctc cgagcccacc ggccggaacc
     3301 ggagcgcggc gttcggcgcg cgcagtacat cggcgtgccg gtccaccaga atcttgacgt
     3361 tggcggtcat ccccggaaaa agcttaaggt ccgtgttggc aacgccgatg acgacgtcgt
     3421 acgtgactac attctgcacg ttgatgggcg cttgccggat ctgcgtgact tgtccgcgaa
     3481 atacctcccc agggtatgcg tccacggtga aggtcgcggg ttgccccacg cgaacacgcc
     3541 ccacgtccgc ctcgctgacg ttcgtgtcca cctgcatctt ggtcaggtct tgcgcgattt
     3601 cgaacagcgt cggcgcctgg agactggcgg ccacggtctg tccgacatcc acgcgcctcg
     3661 cgaccacgac cccgtccacg ggcgccgtga tataagtgtg gctcaaatcc aattgggcct
     3721 gttcgagcgc ggccgtggtc tgcttgactt gggcccccgc cgtggccagt tgcgtcctgg
     3781 agacttcgat atcagcctcg gcggagcgga ccttttgcac cgcggctgct tgttgagcct
     3841 cggaagcctg ctgcatcgcc acggcggtat cataagcggc ctgcgccgtg tctttgtcct
     3901 ggtccgcgat gacgccggct tggcgcagca ggacggccct gtcgagcttg ttcttagcgt
     3961 cctggacgga gactttagcc ttcagaaccg acgcggcggc gtccgccacc ccggctttgg
     4021 cgctggcaac ctcggcctgc gcgcgctcga cgacggcacg cgcgttcacc acggccgcct
     4081 tggccacgtc cacgttcgca ctggcctgag tgacgcgagc ctgaaatgct tgcggatcga
     4141 tgcgcgctac cagttgtccc ttcttgactc tggtgttgta gtcggcatct agctccagaa
     4201 tgttccccga cacctgcgtc ccgacttgca cggttaccac ggcgttggga cttcccgttg
     4261 ccgagacgct gtaggcgacg tcgcctttgt ccagggagac ggtccgatat tgcgtgtcac
     4321 cgtgcccccg caggaccgtc caactcccgc cgaccgctac agccacggcg gcgaccgtca
     4381 ccaggaagac ctttcggttc ggcgcccgca tgatttctac tacccccaca tgtaacgatg
     4441 ctgatcgggg gtcgcacgtt agcgcctaga aggcgattcc acgtaaagac tatgtaaagc
     4501 cgcttcggct acgggcaaac gacgaaatgt cagaacgtgt actttaggcc aaactggatg
     4561 tttcggggta agccgatcgt gctggtgatc actccgaaag tggcgggcga cgagatgctt
     4621 cgtcccggag gaccgaagtt cgagtggttg aggatattga agaactcagc ccggaagtcc
     4681 aatgcgtgag actcggagag actgaacttc ttgtcgagag cgaaatccag gttcttgaag
     4741 gccggggcgc gctcggtgcc gacgccggcg ttaccgaaag ttccaagggc cgggacgcca
     4801 taggcgcaag cgccgtcgtc cactccgggt gtcagacaga acgaattgcc ggtgccgaac
     4861 cattggtcga tagtctgtcc ggtgtacgtc agaggcagga attgatcggg gcgtgttgac
     4921 ccgcggacgg cctggttgct tacatcgctc gaaaggatag taatcgggaa accggagtgg
     4981 aggcttgcca catagctcat cgcccaattc ccgagaatga ggttccccgg tttgctcgtg
     5041 tgctggcccc agcgtttgtc tcttccgaag ggcagttcat agtagccgcc caggctgaag
     5101 acgtgcctgg catcgaaaaa ggccggaccc cagtccgctt tccgatcata cgcattctgc
     5161 cagtaggcgc cttcgctcgc cactccggca gagccgtagt agcccagatt gtcggtcagc
     5221 gacctgctgt atgtgtaggc agacaggaac tccagaccgg aggcgagtcg cttgcgcgcg
     5281 ctgagttgaa gcgcccgata ggacatcgtc gagctcgagt ccgtgccgct gatgttggta
     5341 atcagcggat cgaacgcgta gagcggcctg cgctgctgca gcggcagcca ggtatccacg
     5401 ggacccgtac caggcaacgg ttggttgaat tcgcgcgggt ccaccaggtg cgtgccacgc
     5461 tggccgacat atcctgccgt aaacgagaga gtcttagtga actcatactg cccgctcacg
     5521 ttccactgct ggatgaaggc cggccgcagg ttggggatcc acgcccggac ctgcccggag
     5581 agctggtttt gcggctgaac gtcggtgaag ccgctggtga tgctgcccgg cgaggccaga
     5641 tcatacgtgc tgttggattc aaagaaaaac ggagggttga gcggcaaccg gaggttggcg
     5701 ccggtgccct ccatgaaact ggtgatgccg taaccggcgc gcgccacgaa cttgttggag
     5761 agcatgggcg gcgtccacgc cacgccgact cgcggcatga actggcgata gtaggggtca
     5821 tacagcgcgc gcgaattgcc gtccttgccg gcgaacaact gctggcccgt gaccagatct
     5881 atattggact gccggtcggc aacttcgatc aacggctggg cgtactccca ccgcagccca
     5941 aggttgagca cgaggtcagg ccggagcttg aagtcgtcct ggaagaacgc cgcgatccgg
     6001 ctttgacgtt gaccccagag ccctgtctga cttccgcgtc ccttggaacg caggtcatcc
     6061 aacagaaagt cggcaaaggc ggacccggtg taatctcccg tgtaggagaa agtgccgagc
     6121 aagccattgt tgccggcgta aaaccggttc tgctggtagc ggataatctg gaagccagct
     6181 ttgaaggtat ggagacggcg ctgccagatg aagttgtcac tgaattggta agtgttgtcg
     6241 taagtcgaac tgtccgtcgc cgctgccccg atggaactca gtccgtcccc gagtgagacg
     6301 gaactggcgc cggcgatagg ctgactgccc ggaatgccaa gcgtttggtt gccgttggct
     6361 ccgagtacgc cggtaggatc agtggtgaag aacttcaaaa ccactcgatt gaaaccaaat
     6421 cgggcttcgt tgaccgtctg cgggttgaga gtgcgcgtcc aattcaggac tccgccggtt
     6481 gtcggcgcgt ttgttcctcc gccgagaaag acgggcagac cgacggtggt gtcgcctgag
     6541 cggtaccggg cgatcgtaaa ccgtccagac aaccggtcgc tggccgtgag ctgagtgtcg
     6601 atcttggcgt cggcctggtc attgtcgagg aagttaccgc tcgacgagat gtaattgttc
     6661 gttacgccga tggggccggt gccggtcgcg ttgggaagcg gatacaggct agggttggag
     6721 aacaaggcct tggcgaccgg gttcgcaatg cggtcacgag ggatcacgtt tccggggaaa
     6781 ggtaaattgg tgaccgggtc ccgaatcgtt tgcgaaaatg ccgacaagtc tccaacacga
     6841 tagggcgcaa gcgcgaccga tgccagcgcc gcaccgctgg ttgggttgcg cgtgccctga
     6901 tagtcgataa agaagaaggt gcgatcctga acgatcgggc cgccgagcgt gccgccaaag
     6961 gtgttcatgc gaagggcacg cttccgggcg ccagaccggt tcgagaagaa gctgttggca
     7021 tccaggcggt cattgcggag gaattcgaac aggtcgccgt ggagggcgtt ggtcccgctc
     7081 ttcaatgcga cgttcacgac tgctccgttg ctgtttccga actcagccgg ggcgttgccg
     7141 gttatgactt tcatttcgcc gatcgcgtcg acattcgggt tgtagccgac taggttgtct
     7201 atgggatcgt ccacatcgat gccttcgaga agaaagttat tggtctgctc ccggttgccg
     7261 tttacataag gtctgccacc ggagaatgcc cgcgagggag tcgtaaaaga ggtggggttt
     7321 ggcgtaacag caccggggat caacaggctg agagcaacga aatttcgccc attcagcggg
     7381 agagtgctgg attttgcact gctgatcgtg tcgccggtgt gcgtcgtgtc agtctggaga
     7441 acaggcgcga cgtctgtcac atcaacggat tgcgagatct ctccgacttg catccgaata
     7501 tcgactcgcg cagtttgcgc tacctcgagt ccaaacggct gaatattcgc ggtcttgaat
     7561 cctcttgcct ccacggtgac ggagtagcgg ccgatcgcga gaaatggaag gacataggcg
     7621 ccagacgcat tagagacggt agtcgttctt acgccagttt gcgtgtttgt cgccgtgatc
     7681 ttagctcctg tcatgacggc gccggtcgga tcggtaatgg ttccggtgat ggttcccgtg
     7741 gtggtttgcg ctgagcttgt cgcgatgatc gttcccagca gcagtgtcag tgtttccagc
     7801 cttcgtaaca tggtgatgcc cctccttcac acactagcga gaccagttaa ggtccatgct
     7861 ttcagcgtaa gcgagagaag gtcaaaatca cgccaaaaca gagggaaggt gtgtcaagaa
     7921 aacgtcaaga ttccgagcga agccgatagc cgacgccggg ttccgtgacg atgtagcgcg
     7981 gacgtgccgt gtctcgctcc agtttgcgac ggaggttgct aattgttacg cgcagaaggt
     8041 gtgaagcatc gtggtagtaa gcgccacccc atacctcgtg aatcaattgc cggtgggtca
     8101 ggaccttgcc ggcatggtga acaagaaccg ccagcaaatc atactcggtg ggagtgagtt
     8161 gtaccggctc gtctgaaaga cgtacttccc gacgcatcag atcaaccaac aagtcaccgc
     8221 acacgaaggt tgggctatcc ccgaccacgc aggcacggcg caacgcggcg cgaattctgg
     8281 ccagcagttc tccggggccg aaaggcttcg tgaggtagtc gtcagcccca gcatccagcg
     8341 ccgcaatctt ctgggtctcg tcgtctctaa cagacagcac gatgatgggg gttttagccc
     8401 attcgcgaac gcagcgaatg atttcagtac cgtcaacgtc cggcaggcca agatccagca
     8461 ggattacgtc gggccggacg gatcgtacct gttggatgct ctgttcaccg tcttcagcct
     8521 gatacacggc gtagccatgc gccgaaagcg aaacgttgag caaggtccgg atggcacgct
     8581 cgtcgtcaac gacgagaatt cgcggagggc gattatcgcc cgcaagagcc cgagtgtcgt
     8641 gctcagtcct tgatatctct gctatcttcc atcgcatacg ccgccgatca agcagctgtc
     8701 agctcctgag gagcaccgga gtaaggtacc ggcaggaagc cgaattcgcc cagtcgcggt
     8761 atttcaaaag tgatgagcgt gccaccctgc actcgccgct cggcccaaat ccttccactg
     8821 tgagcctcca cgaagccttt acaaatgtaa aggccaagtc cgattccatt agagttagca
     8881 gcgttcccgg cgcgctggaa tttctcgaag atccgcatca gatcgccaga ttctattcca
     8941 atgccccggt ccgccacacc gacccttagg tggccgccct cgattctcgc gcaaatttcg
     9001 accggccgat cgacaggcga gtaacgaagg gcattgtcga caagattcac gagaacctgc
     9061 gaaacgagca cgaagtccat gtaaaccaga ggtaagtccg gcgggatgtc gacgctgatc
     9121 cggcggcttg agatcgcgct gcccaattgc gccagcgcgg agcctatgag gtcctgcatt
     9181 tcgcagggtt cgattctcaa tcgaagcgca cctgcctcga gtctggtcac gtccagcaga
     9241 ttgccgacca ggcgattcaa ggcgacggac tgttcaagag cactctccag cagttcgttg
     9301 cgaactgact cggaaagtgc cggaccgtcc tcgatgaggc tgttcagagc gccgttaatc
     9361 gacgccagcg gagtccgcag attgtgagaa acagagttca tcagcgcctt gtgcagacgg
     9421 ttcctctctt cgacccagtg cgcctcttgc gctttctctt gcagtcttgc cctctcgatc
     9481 gcgcatgcag cggccctgcg cgcgttctgc gtcgcttcgc tcgccaatct gctgatgatg
     9541 agaccggcga caagaaaagt gacaaatgta atcaggtacc agtcgtccct gaaggcaaag
     9601 cttctatagg gcggtacgaa gaaataagca aaaacgattg ttgatgttac ggctgttacg
     9661 acagctggta gcagccccca ctgtagtgcg gttactacaa ccgcaagcag gtagaccatc
     9721 gccaggtttg tggcagtcat ctggtgttcg atctggaggc ccgtcaacgt gatcgctaac
     9781 acaagcgaaa ggctgacaag gtaggaaaac catgggcgtc tgcggacatt gccatgaacg
     9841 actcccagcg cgatttcggg cgcagcaggc gcggccggat cgtgcatgcc gcaaagccgg
     9901 cggagcaaac gatcgatgcg atgtgccatt cctctggctc ccgccaaatt cccccgccag
     9961 ccaaacgttt tacaagcgta aacccgtcag cgaagtggtg cgaaaccgcg caactccagc
    10021 ttttctttca tagacgccta ccggcagcac gaggtttctt gccgcgtggc cgaggagagc
    10081 cgactgggct cctctgggga gcgcctcagt ctgttatcaa tggggtggag gggaattgac
    10141 aagattctgc ggaatcgcct cggcggcgat cattgccgga gttgtcctgt ttctgattcc
    10201 gtgccgcgcg gagacgattc gggtcagcaa ttcctacggg ggcatcagtg tctttgtggt
    10261 gaccgggagc cagatccgag tgcggcaatc gagttccgta cgacctgttc acgccgatga
    10321 tgtgcagatc acgaaggcgc ccgggctgat cgcggtggat tgccagcctg ccgacggggc
    10381 gcaggtggac atcgggatcg gactccccta cggcgccgca attgaggcca ccacgaagaa
    10441 cggcgctgtg tccctggaag gactgattgc acgagctttc gtaagaactg tcggcggcga
    10501 tatcctgctc acagcgccgt gggaggctac ccggctgact atttcatcgc gcgccgcgcc
    10561 gaggggattt gcagcgccgg gcggtatcaa gctttcccag cgaacgcaag acggagcctg
    10621 gctgctcgag gacaaacttc cagagtcgaa ggtcacttac ggtgtgattc gggtggaggc
    10681 cgaatcggcg ggccggattg ttctgtcgga gatgcccatt ccggcggatt cgccagtcaa
    10741 gatgcactgg caggcgccgg agatcctgga tggcatcatg aatggcggtt tacggaatag
    10801 ggcttcatct tctcgagcgc caatcaaccc gccagcgccg gcggctgccg ccgaagcggg
    10861 cacgcccgtg ttcacgtcgg atgtgcgcat ggtgaacgtc accgtcgcag tcttcgacgg
    10921 cgaggggcat catgttgacg gtctgaagcc tggagacttt cagatcctcg aagacggtgt
    10981 tccacagaag cccgcgattg ccggttccga ggaggtgccg ttcaatcttg ctattctgct
    11041 cgatctgagc ggcagcacgc tcagagaccg tcccgcgatg aaagaagccg cgcgccgttt
    11101 catcggaatt gcgcgtccac atgatcgtgt cgccgtctac gcgctggcta acgattcgtt
    11161 tcacgtggtc tcacggctga cggcggacag ggagcccctg ctcgcagcca tcgaagcaat
    11221 ccccgaagtc tcgggcggct cgccgctgta cgacaccgtc gttctcgcgt atgcggagga
    11281 gttcttggag cacccgaaag agaggaacgc actgatcttc atcagcgacg gcgtagacaa
    11341 ccagattcac ggcgtctcca cgccgtcgaa agtctcattc cgcaaacttc agcgcgccgc
    11401 cgcggagatg accgcactga tctacccggt gtacctggac cctttcaccg tggcgcctcc
    11461 gccgcgatgg gcggtcagcg cacgaagcgc gctggggaaa cttgccgaag cgaccggagg
    11521 gcgactgttc cgagccagct cgattcagga cctcgatccc gtgtacccac aggtcgccga
    11581 ggagttgcgc agcgtgtata cggtggcata ctatccggcg aatcaggact tcgatgggaa
    11641 gtggaggcgc gtcgaggtgc gtgtgaaccg gccgggggtc aaagtgcgga cccgcgccgg
    11701 gtacttcgcg aagtagccgc cccaacccgt aaatgcgtgg catagattga agccggttga
    11761 gtagcgattg cgcggcgaga ggtaagaggg tataagatca tcgcgtggta cgaattcttt
    11821 ctatctcctt tgttctccgc ctcattcttt gcgcgccggc gcacgcttcc actcccgttc
    11881 tgcccgatct gcatgcgccg ggcggcgggc cgatccgcgt ggaggtgaaa aagggcgcac
    11941 gcacgatcgt gaccaccgac tactggcggt tggagttcga tctgcgccgc ggcggtgtgc
    12001 tggattcgat cgtattcctg cacggctccg gacggaatgt gctggtgcgg ccgttccgca
    12061 cgcatgtcgg tgattggtca gatcaggacg cggggatggc tgaagttgcg tcctcgcaag
    12121 accgcgacgt cgtccggctc gaattccgcg ggacgatggg cgcgcctggc gaccggaagg
    12181 ggccggtgtc cttcacaacg acctggacgc tgtcgccctt tgcggttcga gccgagcatg
    12241 ctgtgcgtct ggccgaggat gtgagcgtac gctcggcggg catcggctcc atgtccgtca
    12301 gggccgagct gaacgagttc tcgtggcggc cgggagtcgc gacggattcc cgacgcatgc
    12361 acctggcgag gtcgggcaga gcagccggcg agggcgcgca agcgatcact gactacaaca
    12421 cgccgttatg gctgctggtg tttcaccgcg ccgtggaggg tttcgatctc actccaggct
    12481 ccgattacga gacatggcag ggccgcctgg gggcagacgc cggagggcgg ctatacgagg
    12541 tgaaagcggc gagcgacggg tcatcggtcg agattttgag agagccgctg cactcgaaag
    12601 agcctgtgac tgcgcgcaag ggcgaataca agttcgctta ctatgtcggg ctgccacgga
    12661 tcgtcgagcg atccaaccga aagtggcggc acctttcgct cgggaatcga ccgtttcctt
    12721 ccgatgacga gatccggcgc tgggcagaga acggcgtcaa cgtcgtccgg tatcacaacg
    12781 atttctctcc agatgggaat ttctggcacg atggggcgtg gcctccgtac gacgagaagg
    12841 gcatgatcga actgcgccgg gtcatcgcgg cgtgcaagcg gaacaagatc gcgatcgtgc
    12901 cgtacttctc agtctacgaa tttcacccgg aagcgcaggg cttcgaccgt tatgctggcg
    12961 agtggatgag gatgggggat ccgggcggcg cgatgatcca caacctcgtg tgcgccaaca
    13021 acgaagggga gtacggcgcg cagatgtgcc ctaactccgg ttggctggag cggcgcaaac
    13081 aggacattga gcgggcctac cgggatctgg gtttcgacgg catctactac gactgggtca
    13141 agacgctttc ctgcttcaat cccaagcacg gctcggcgca ccacctgagc acggaccagg
    13201 tggtggatct aatggcctgg acgcggcggc tggtgagacc gaacgggatc gtcgtgctgc
    13261 acatctacgg gcacacggct tcactggcac tagagaactt cgccgaccta gtggtgaaca
    13321 tggaggaggc gtcggcgacg gagcggtgga tgaagattac cgacgtgccg atcatcacgc
    13381 ggctggcgga gagtatccct cgctcgccgt gcccgtccta tcgccccgac catacggtag
    13441 accggaaccg gaataacatc tcgcaacttg tggtgctggg gatgttcccg gcatggcgtg
    13501 cgacggggcc gtcggtggcg ccgccgggag gtccggggta cgaattgacg ctgaagctgt
    13561 tccgtgattt caagccgtac cgtctggaag cgtaccggtt ccacgatgcg ttctcgggcg
    13621 cagtgaagac agcgtcagat gacgtttacg gtgcggccta cgggtcggcg gaaccgccgc
    13681 tggtagtgat ctcgaatacc agtcaggaaa agaggagcgg cgtttcctgg cgggtggacg
    13741 cctcgaagct gggcttcgga actccagcgc gggtgacgtt gaaagacaca cggtccggcg
    13801 aaacgcgaac actgccggtc ggcgcgttga gcgacggcag cctcgcgatc gatctcgatg
    13861 gctgggaata cgggatcttt gagatccggg cggccaagta agaatcagga gactggaggc
    13921 tgtgcgaaat ccgatttcaa gacgggcgct cctggctggg ctcgcggctc ccagagcggc
    13981 gcgacctccg agcttcctga tcatgctctc ggacgatcag ggatacgggg acgcaggctg
    14041 ttacggcggc gagattccga cgccgaacat cgactcgatt gcgcggaacg gcgtcaggtt
    14101 cacgcagtat tacgctgccg gaccggtatg cacgccgtcg cggtacggac tgctgaccgg
    14161 gcgctatccg gcccgctcgc gcgacaacct gctcggcgcg ttgatgccag ggtcgaagaa
    14221 gggtattcat tcgggagaga cgacggtcgc ggacgtgctc aagggccggg gctaccggac
    14281 ggcggtcttt gggaagtggc acctcggaga cgcgctgccc gagttctttc cgacccggca
    14341 cgggttccag cggttcgacg gctttgttca cggctgcatc gatttcttcg acttcacgta
    14401 tggcggaatg aagtgctggt atcggaacga atcgttgttt cagccacccc gtggatacac
    14461 gaccgactac atcacggacc gggcgatcga gtttctgcgc gacctccgga accggccatt
    14521 cctgctgtat ctcccgtaca acgcgccgca ttacggaaag acgtggtacg accctgtctc
    14581 gcgaaaggca agaaacgcat tacaggctcc ggaagactac gtcgcgcggt ttgcacatat
    14641 cagagataag gaccggcagg gatatgcagc gatggtagca tcgctggacg acaacatcgg
    14701 acgcctcctc aatgcggttc gggaactgaa acttgaggag gacacgctgg tggtcttcgc
    14761 cagtgataac ggcgggtcgt ttccgtacgg tgggagaaac ggaccgctgc gaggccagaa
    14821 aggggagctg ttcgagggcg ggatccgggt accgtgcgct atgcagtgga agggccgaat
    14881 cgctcccgga tcggtcacca gccaacccgc cggtggtgtg gatctgatgc caacgctttg
    14941 tgcgctggcc gggtcggcgg ttccgaagca ggtggacggg cgggacctga gaccggttct
    15001 gttcgagcgg cggagtctcg aacgtgagct gttttggcgg acaggcgacg ccgatgcgta
    15061 tcgctccgga gactggaagt acatcaggat cgccgggggc agggaactgc tgttccacat
    15121 cgagagcgat cccggcgaga aagtggaccg cagctcggac actgccaggc tcgcgcagat
    15181 ccgggagcgt taccgtcggc tgctcgcaag cgggctggag taggttggaa tacacttctg
    15241 atgtcgggag tgaaaaccgt gaaaacgtca gccaagctgg atctctacaa ggaattcgag
    15301 aagcagtatg tgaaaccgac gaagcccatc ttcgtaaaca ttcccgcgat cccgtatctg
    15361 accacggacg ggagcgggaa gccggggagt gaggattttc agcggaagat cggcgccctc
    15421 ttcggggtag cgtacacgat caagatggcc cggaagtccg ccggccggga ctacaaagtc
    15481 tgtggtctgg aggggttgtg gtggttcgac gatgccaata agccagtaag tgaattgccg
    15541 ctggacgagt ggaactggaa actgatgatc cgcgtgccgg attttgtcac cgcccgcgag
    15601 gtcagacaag ctgtcaccgc gttagcgaaa agaggcaagt tgcccgatgg ggtcgaggtg
    15661 cggctcgaga agatcaagga aggacagtgc gtgcagatgc tccatgtcgg cccttaccag
    15721 gaagagaccc gcaccgttca ggcaatgaag gagttcgcgg aggcgaacaa gatgcgtttc
    15781 cacggaccgc accacgagct ctacatttcc gatcccaatc gcgtggcttc ggagcggtta
    15841 cggacgatcc tgcgacaggc tgtccgcctc gcctaattca tggggcaggc gttggagtgg
    15901 cggcttgtgt ggccggcctc ggcggattca tcaagcgatc cagaaagccg cgcactttcg
    15961 gcgcttcgga tgagtccggg tgcagggcga ggaattcctt gagttccttg atggcgagga
    16021 cgcggtcccg acgccggata tagatctcgg ccatcaggag ctgcgggttg gagaagtgag
    16081 agggatcgag ctctctggcg gcgttgaggt atttcagcgc ctcgtcgtcc tgcttaagaa
    16141 gaaagtacgt catgccgagc tgtgagttgg cgagggcatc gcgcggccgc gtttcgacgg
    16201 cgtacttatt gaacttcagt gcttcctcgt gcttgccggt gtacagcaag actccgccga
    16261 ggttgaccgc tggaggaaac gcgccggggc gcgcctcgag cgctttacgg aaatacgtct
    16321 cggcttcctc gaactttcgg gcatggtagg cgatggtccc gagattattc catgccatca
    16381 tgtatccggg cgcgatctcg acggcgcgct caaggtggcg aacggctgct tcgacgtcgt
    16441 tgccgccgag cttttgctgg gcttcgtcgt actcgcgttt ggcgcgatca gggatcgaga
    16501 ggtcgccgac cgacacggtg ttccgccgcg cggctaaacg gccggcggag accggagtcg
    16561 ggttgaacgg gatgaccgcg tggactcggc cttcggggtc ggcgaggctg ggggttacct
    16621 ccactgtgcg ccgggagact ccggcgccgg ctatgaacac cgtgaccgaa taagggccgg
    16681 gttcaagttt cttgaacttg aagcgacccc ttgcgtctga gatcgtgctc gcggtgaatg
    16741 agccgaaggt agcgtcgatg gtgacggacg ccttgcccgg ggcaggctcg atctgaccgc
    16801 gcagttccag ggtccggtcg gcggcgccgg cggcgagtgt caggagaagc aagatggtcc
    16861 cggcgcgcat agccttcggg aagatcgtac cgcaacgggg tcgagtttgg aagaggtcat
    16921 gagcggatac gagatcctgg cagccacttt cctcgcattg catctgcttt ggatcgtctg
    16981 ggtgattgcc ggcgtcgtgg tgacccgcgg ccggccggcg ctggtggccg gccatgtggc
    17041 gtcactgctg tacggaatcg cgatagagac cggtccatgg ccgtgtccgc tgacgctggc
    17101 agagcagtgg gcgcgggaga aggcgggcat cacaccatac cgggagggct tcatagttca
    17161 ctacctcgaa gctctggtct acccggacgt gccacaggaa tggctgacgc ggggtgccgt
    17221 ggcggtttgc ttggcgctgc ttggagtcta cgggcggaga tggtggagat ggacgcgcgg
    17281 ccgtgtggtt gctcgccggg agtgagtcag tcccgatttc tcccgcgaac gactcagggt
    17341 gtggaggtca aaacccggga cagacaccaa tttcccaaac gcagcgaaat ttggagtcag
    17401 tcccaggttt tgttagcaac gacgtttctt ttacattgga ggtctatgaa agcgctggtg
    17461 aagagcaagg cggagcccgg actttggctg gaagacgtcc cgatgccgga aatcggcatc
    17521 aatgacgttc tgatcaaggt caagcgcgcc tcgatttgcg ggaccgacgt gcacatctac
    17581 aactgggacg attgggcgaa aaaaacgatt ccggtgccga tggtggtggg gcacgagttc
    17641 gtcggagaga tcgtcaagac cggcgcgaac gtttccgatt tctttccggg tatgctggtc
    17701 agcgccgagg ggcatgtggt gtgcgggcgg tgccgcaact gcctcgcggg gcgccggcac
    17761 ctgtgcaaca agacgctcgg agtcggcgtg aaccggccag gcgcgttcgc cgaatatatc
    17821 tcggtgccga tgacaaacgt ctggcatcac ccggacagca tggacctgga ggtggcgagc
    17881 atcttcgatc cgttcggcaa tgcggtgcac acggcacttt cgttcccggt gctcggcgag
    17941 gatgtgctcg tgaccggagc gggtccgatc gggatcatgg gcgcggcggt ggcgcgccac
    18001 gcgggcgcgc ggtacgtcgt gatcaccgac gtgaatccgt ggcggctgga acttgcgaag
    18061 aagctcggtg tgacgcgggc agtaaacgtc aagacgacac gcctgaagga cgtgcagaaa
    18121 gaactcggga tgaaagaggg cttcgacgtc ggcttcgaga tgtcgggcaa cggctcagcg
    18181 tttcgggaga tgctcgagaa catgtgccac ggcggcagga tcgcgatgct tggcatcccg
    18241 ggcggcgaga tggcgatcga ctggaatacc gtcatcttca acatgctcac catcaaaggc
    18301 atctacggcc gggagatgta cgagacgtgg tacatgatga acgtgatgat cgagtccgga
    18361 ctcgacatca agccggtgat cacgcaccgc ttccactaca cggagtacga gaagggcttt
    18421 gatgcgatgc ggagcggcga atccggcaag gtcattctga actgggagtg agcggatcga
    18481 agatccaggc gggcatcggc ttcaactctg cggcgacgga cggggtgaac tccatctgcg
    18541 aaaggacgtc ccggcgcagg tcgattcccg gtgcgacttc gatcagctcc agcccgcccg
    18601 cagccagctg gaagacggcg cgctcggtga cgtagagaat ctccctgccg tgcgcggtgg
    18661 cacggggtcc gctgaaagtg acgtgcccga cgtcccgcac gaacttgcgc gtttcgcctt
    18721 ccgcgcgaac ctgtaaccga ccgccctccc atcgcacgtc gaggccgcct gcgctgaacg
    18781 ttccgacaaa gacaacgcgt ttggccgtct gagaaatgtt ggtgaatccg ccggcgccgg
    18841 cgatgcggcc cgcaaagcgg ctgacgttga cgctgccgca gcggtcgcac tcggccatgc
    18901 tcaggaccgc gatgtcgaga ccgccgccat cgtagaagtc gaacatgtag ggctggtcga
    18961 tgatcgccat cgggtggctg gaagcgccga agctcaatcc gccggcggga attccgccga
    19021 tggcgccggc ctcgacggtc agcaccatgc tcctgagacg gtcttgcgtc gcggccacct
    19081 gtgcaacccc ttcgggaatt ccgatgccaa ggtttacgac gtcgccgtcc cggatttcag
    19141 cgaatgcccg gcgagcgatc cagaggcgga cgccgttcag agcaggggat tggccgccgg
    19201 cgcaaatatc gccgggttcg acgtaggccg gattgaactg ctcggcgaag gtctgcatgt
    19261 ggttttcctg cgagcccatg acaacggcgt cgacgaggat gccgggaatg cggatcgact
    19321 tggggtcgcg ggagaagtcg ggcacaatgc gctccacttg aacgacgacc gctccgccgg
    19381 agtttttcgc cgacagggcg atcgagaggg cctcgccggt aagcacctcg tcatcgagcg
    19441 agatgtttcc ataagaatcg gcggcggtgc cacgaagcag cgccacgtcg atgggaaacg
    19501 ccttgtacca gagccattct ttttcgccga ggcgaacgac ttcgacgagg tcctccgtgg
    19561 atcgggcgtt cagcttgccg ccgccgtgac gcgggtccgc gaaggtgtcg aggccgatgt
    19621 gggtgaccag gccgggattg ccggcggcga tggcgcggaa caagtgggac atgacgccct
    19681 gcgggaagtt atacgcctcg atttcgtctt cgagcgcgag tcggcccagg cccggggcga
    19741 gattccaatg tccgccaatc acccgccgca ggaggccctt atgtgcgatg tggttgaggc
    19801 cgcggctctt gccgtcgccc tgtccggcgg cgtacacgag ggtcaggccg cgggggctgc
    19861 cttccaactg gaagcgccgc tcaagcgcgg tggtcagttc ctcgggatgg ccgcagccga
    19921 cgaacccgct ggtggcgacg gtagcgtcgt cggggatgag gcgaacggct tccagggcgg
    19981 tcagaatcgc aactttcttc acgcaaacca gcgtatatca cgcagggagg aggcggttgt
    20041 atactgaagc cctagcccca cgggctctct gcccgcaggg gagaactcat atagagagag
    20101 ccatggaaaa caaagaaatt gatcgcagaa cgtttctcgg agctgccgcc ggcagcttca
    20161 tgattatgaa gccggagctg gtccggggca gccagcggaa ttcggcagtg cggatggcgc
    20221 tgtttgggtg cggcgggcgc ggcacgggcg tggcggaagg tttcacgctg gaaacgacgg
    20281 cgcagtatgt cgctctcggc gacttgtatc aggagcagac cgagcgggcg caggcgacga
    20341 tcaacaaggc cgcggagaag aaagggaaga cgcccatcaa ctcggccatg ctctttaccg
    20401 ggccgaaagc gctcgacaag ctgacggcga tgcgggagct cgacgtcatc cacattgcga
    20461 cgccaccgta cttccacccg gcgcacttcg agcaggcggc agcctcgggc aaacacattt
    20521 accttgagaa accggtggca gtcgacgtgc ccggcgcgaa gaaagtgatg cgcatcgggt
    20581 cacagatcgg caagaaagcg agtgtcgcga tcggattcca gttgcggcac gctacccctt
    20641 acgtgcaatt ggtggaacgc atccggcgcg gcgacgttgg tgacatcgtg tgcggcctgt
    20701 gtcactacta cgccggagcc atcccgcaac cggaccgacc gggggcgggg ccggctacga
    20761 agcggctgcg caactggctg cacgaccgcg tgctttctgg cgacatcctg gtggagcaaa
    20821 acatccacct gatcgacacg acaaactggg tgctcaaagc gcaccccgtg atggcgcaag
    20881 gcgtgcgggg ggcgaagggc cggcgcgacg cgggcgactg ctccagccac ttcaacgtga
    20941 actacacgta cccgggcgac attcacatca cgctgacatc gacgcagttc atcgagggct
    21001 cgtgggacgt cgccatgcgg tacttcggca cgggcggtaa cgcggagatg cgctacgact
    21061 cgccggttcg gatcgacggc ccgaagaaat gggacttccc gatcggccag aaggccgggg
    21121 agattacgga cgtcggcgcg gcggcagcgg gcgccttcaa gggcgcgctg gatgatgcgg
    21181 acacgatgaa gcagcgctac gtcatcgaga gcatcgtctc cggcaatcac atcaacgaag
    21241 ccgagcaggg cggcatctcc actttgagcg cgatcctggg tcggcaggcg gcttacactg
    21301 gccgcaactg gagctgggac gagctgctca aggtggaaga ggtctgggac gcgaagatcg
    21361 acgttgcgaa ggtgtaagaa gcgggggttc cggaaaacac ggggacagac acccagtttc
    21421 gcacaggcgg cgaaatggga gtcagtcccc ggttttccgc aagcgcggtt cgcactatga
    21481 agaggagaag ctttttcgga tcggtgttcg cggggcttgg cagcgcggcg actgcgcggc
    21541 cggcgaaagc gaaggtgggg gacatccctg tgcggccgct cgggcgaacc ggacagaagc
    21601 tcaccatcgt cggcatgggc ggagcgcggt tccacctcag cgggtgggag gacggcaccg
    21661 tgctggtgcg gcgcgcctat gatctcggga tcaactactt cgacatggcc cgaagctact
    21721 ggaacggccg cgcagaggaa gtttacggcg ccgtgctgcc cgaatttcgc aagaacgtct
    21781 tcatcacgtc gaagtccaac cggcgagcgc ggaaggaggc ggaggccgaa ctggatctgt
    21841 cgctcaagct gatgaaaacg gattacctcg acctatggca aatccacggc gtgggcacag
    21901 acgaagaagt cagccgcatt ctcggcccgg acggagcgct cgaggcattc acggcggcaa
    21961 agaaagccgg caagtgccgt ttcatcggct tcacaggaca ctccgacccg aacgttaacc
    22021 tgaagatgct ccgtggtttc gacgggtttg acacgatgct gatgccgctg cacgtggccg
    22081 atacgttcta cctgagcttc gaaaagctcg cgctgccggc ggcgatcgag cacggcatcg
    22141 gaatgttggg aatgaagatc ttcggcaacg cgttcctgct gcgcgcattc agcgtgccgg
    22201 agtgcctcca atacacgctg agcctcccgg taagcgcggt gacgctcgga ttctcgacgc
    22261 agggccagct cgaagacgac gtgcgcatcg cccagaattt caagccactt cccgcggaa
//
LOCUS       DATIFQ010000495        15398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2088550, whole genome shotgun sequence.
ACCESSION   DATIFQ010000495 DATIFQ010000000
VERSION     DATIFQ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 15398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15398
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088550"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VLE22_24885"
     CDS             <1..1030
                     /locus_tag="VLE22_24885"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17708.1"
                     /translation="IGVFDRSAPLPTGGHIEQADGTGWMGMYCLNLLAIAMELAREES
                     CYEDVASKFWEHFLYIANAINHLGEDGAGMWDDQDGFFYDVLHMPDGRHSPLRVRSMV
                     GLIPLFAVETLEPEITGKLKGFTRRLEWFIENRKDLTRNVACMRSPGEGERRLLSIVD
                     RDQLRRVLRVMLDEREFLSPFGIRALSRIHRDDPYVFAVNGAEYRVQYEPGESTTGLF
                     GGNSNWRGPIWFPVNYLIIESLQKFHHYLGDDFKVECPTGSGQLMTLWEVAAEISRRL
                     TRTFLRGPEGRRPVHGSNEVFHNDPHWRDLVLFHEYFHGDDGSGVGASHQTGWTGLVG
                     KLIQQSGE"
     gene            1141..2838
                     /locus_tag="VLE22_24890"
     CDS             1141..2838
                     /locus_tag="VLE22_24890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HSB17709.1"
                     /translation="MQNVFRIVAGCFLAFACQAQTLSFSLLGAQDPQPSARFDGTIAY
                     DPAGRQIFLFGGQDIAPKNDLWVYSLARRRWTEVQVSSARPPARFGHTLIFDPIRRRL
                     VAFGGQAGGFFSDVWAFDIVRGLWEQLSANETGPSRRYGHSAIYDAARDRMVISHGFT
                     DAGRFDDTWAFEFTSGSWRNISPSGTRPLRRCLHHAVYDSANNQMLLYGGCSSPVGPC
                     PLGDLWSFDLTAGRWTERTPQSSPPAREHYGMAFDAVRGLLVVFGGNGNGLLNDTWRY
                     DPRSGTWQQPPVEGPVPSPRRRHETAYATDRGTIFFFGGVTASGLTNELWMVGPGFAH
                     DRPEVPQNGVVDAFDGEGGGVAPGEIVSIFGSGLGPVTGVALEFDPQTGQLPTSGPGV
                     SVTWNGMPAPLYFVRSDQLNVQVPYELAAATEASLVVTVNGQSSDPITLPVVPTRPGL
                     FPGIWNQDGSVNSTDNPAPAGSIVVLYATGQGVTDPPSRTGAYPVGLYPAPSAAMSLS
                     IGGTDAEILFRGQAPGTAGVMQINARVPEGLTAASPAAVVLTVGAASSRPGVLLSVR"
     gene            2934..3806
                     /locus_tag="VLE22_24895"
     CDS             2934..3806
                     /locus_tag="VLE22_24895"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSB17710.1"
                     /translation="MKIVKGRLALAVLLGIASGAPAQVTDKKALTLDGARKIIAAAVA
                     EAKRVDAPGAVIAVVDDGGNLMALERLDNTFAAGANISIGKARTAALFKRPTKVFEDI
                     IGKGRTSMVALNDFTPLQGGVPIVSEGQVLGGVGVSGAASAQQDEQLAVAGAEAAKSF
                     AGAANGAQSSAVSYFNHGTVEAAFAKGAVLFDGSDKYMVHASRRDKPGQAEVHTKDAD
                     IIYVLDGNATFVTGGTAVDAKTTAADEIRGTEIAGGDTREISKGDVIIVPAGTPHWFK
                     EVSNPFLYYVVKAR"
     gene            3829..5463
                     /locus_tag="VLE22_24900"
     CDS             3829..5463
                     /locus_tag="VLE22_24900"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSB17711.1"
                     /translation="MKKNTVRVVFALLPFVVVASAAKIRPEAPSGSPSATIDLAADEG
                     VRLVNGQWRYSDTKIIEVEFRGPGPDGQPTGAPVKTYDYTPHAGTADFDDSQWETIKA
                     ANLNVRRSTGRICFNWYRINITVPERIGGFNPAGATVVFETSLDDYAEVWVDGELSRA
                     LGQVGGSVVGGWNAPNRLVIGRGVTPGQKIQLAVFGINGPLSNPPTNFIWMRYARLEF
                     YEASPEPVAITPREVNVEVVRLDPAMDTIVGPNPKIFKLAEGFRFTEGPVWISDGGYL
                     LFSDPNSNTIYKYTPGSDNSGELSVFHHPSGYSGDDIAEYRQPGSNGLTLDPQGRLTI
                     NEHGNRRVTRFEKDGTLTVLAASYEGKRLNSPNDLVYRSDGTLYFTDPPFGLPKFFDD
                     PRKELPYSGVFSLYKGKLRLVSTDFSGPNGIAFSPDEKYLYVANWDDKKKVVMRYEAN
                     ADGTLSNGRLFFDMTNAKGEDALDGIKVDKQGNLYVSGPGGLWVLSAEGKHLGTIIPP
                     KHPHNLAWGDEDGKTLYLCARSGLYRMRLNVIGVRP"
     gene            complement(5508..6077)
                     /locus_tag="VLE22_24905"
     CDS             complement(5508..6077)
                     /locus_tag="VLE22_24905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB17712.1"
                     /translation="MTTQADTSERLASFEDIALPHTRDLFRTASSILGSAAEAEDAVQ
                     EAYLQAWKSFDRFTPGTNCRAWLFGILFHVISHQRRKWLSRVVLCEPKTFEDTTPYAA
                     PVPDNLTDQAILSAFRSLPQQFAEVVMLADVHEFSYKEIQESLGIPIGTVMSRLSRGR
                     QLLRAQLADHAPAASVRSGGVRAAGAHLS"
     gene            complement(6113..6379)
                     /locus_tag="VLE22_24910"
     CDS             complement(6113..6379)
                     /locus_tag="VLE22_24910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17713.1"
                     /translation="MLRISEGAMGSDSVTFLIEGRVIGPWVMELRTFCERFLGKGYRL
                     TLDLGDVSFVDRDGVAALRDLVSRKATLINCPLFLVEQLKNSGS"
     gene            complement(6528..12029)
                     /locus_tag="VLE22_24915"
     CDS             complement(6528..12029)
                     /locus_tag="VLE22_24915"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSB17714.1"
                     /translation="MEIRGYDVAQEVLRDERYVISRGRGTEDKRPVLLKAAARVPCTV
                     ADAVSLEHEFELLCGLSVAGVPRAYELVQREGGPCLVLEDRGLIPLRRLIETGRPDLN
                     SFLQIARTLCEILAELHRQDIIHGSLTPSAIWAPPDFSVLQLIDFTLASGCSRQIRSS
                     PAILLGGALAYASPEQTGRMNRATDYRTDFYSLGVTFYELLTGTLPFKSGDALELIHS
                     HIAKTPVPPAEILPEVPEQISQIVMKLLGKTAEDRYQSALGLSQDLQICQQEWTERRS
                     IAPFVPGQRDVSDRFLIPQKLYGRDREVGDLIEAFDRAGEGGTAMALVSGYSGIGKTS
                     LIQELYKPIVRQRGYFIAGKFDQIVRNIPYGALIQGFRDLIRQLLTETEEQLARWRTQ
                     LSNALGGGGGVLAEVIPEIELVLGKQQQPPPLGPAEAQNRFRYVFQSFVGALARREHP
                     LVIFLDDLQWADAATLGLLQPLLTSHDIRFLLLIGAYRDNEVDVRHPLVRTVRALKSA
                     GAPLSHIVLGPLQLSELTLLVRDTLHGQLTDVEPLARVILQKTDGNPFFAIQFLKTLR
                     QEGLVTFDHAQGRWTFSVEAIAAAAITDNVVDLMSRRIQRLSPKAQHTLTFAACIGSQ
                     FDAGTLAEISEQRTHDAECDIREALQKGLVLPADDEGGGYTFLHDRVQQAAYAMIPEL
                     QRRQVHLTLGRFLLARWDPGTSEEKLFDIAQHFNHGSNLIDDAVERVNLALLNLNAGR
                     KAKSQAAYQSALIYLQAGLHLLSEERWNSDYNLMWELHIEEAECEYLTGRFPEAETCF
                     DRLVTRARTNLEKARISLLRIVQCENMARYAAAVQYGREGLALFGVAFPSSTEGEQAA
                     LEVELSHIQALLEGRTIHSLVDLPPMQDLELRMVMNLLTALWASAYLVGSQALTRLIS
                     ARMVRLSLAHGNTEESAYGYVTHAITVGPVRGDYQSAYEWGRLALAVNERFNDKKRRA
                     KVHQQFQAHVSLWRRPLETSIQHAREACQAGLETGDFTYAGYGALTETWAALLTNRDL
                     ERFVSDCLASIAVLEKIKMSSLVPPQTVIMNWARALQGRTADRLSLSDDSFQEETYLE
                     TYGTHPFSLTFYHVAKLSLYVTFGAYDRAFEAAQKARQVVQHLSGTIWPVLLDFFYGL
                     TLAALYRDATEEQRGTYLTELTALRDSLGVLAENCAENFGCYSLLLHAEIDRISGHGW
                     EAMASYEEAIRSARAANRVWNEALANELYGRFWRERGNEAIAALYLREASRVYERWGA
                     AAKVRDLEEKGGLAPETGVTDSVSVDIASVIKAARAIAGEIVLEDLLRRLLRITLENA
                     GAQRVVFLDENDGNLHVAAEGTVDREDVRVLQSVRLDESPNLCQSVVRYSRKTGESVV
                     IGDALKDERFSDDPYVRSAKPRSILCVPVVHQGKLGGILYLENSLAADAFKADHIRVI
                     QILSSQAAISLENARIYGEMKQEVARRRQAEEELRSALAEVETLKKRLEAENVYLQEE
                     IRREHNFEEIIGNSPALLELLQKLEKVAPADSAVLICGETGTGKELVARAVHNRSRRR
                     DRPLVKVNCGAISAGLVESELFGHTKGAFTGALERRTGRFELADGGTLFLDEVGELPP
                     ETQVKLLRVLQEGEFEPVGSSRTQRVDVRIIAATNRDLAEAVRAGRFRSDLFYRLNVF
                     PLEVPPLRERRTDIPQLVMFFVSRFAKRFGKAVEGISQDAMDQLVNYSWPGNIRELQN
                     LIERAVVLSPGPVLALERDHLPPFAANQAASSSVIRTAEPTSADPIQAEQPTAIDQVE
                     RRHILAVLEKAGWVIEGPGGAAKLLNLHPNTLRSRMKKLGIQRVRHAIS"
     gene            12138..13568
                     /locus_tag="VLE22_24920"
     CDS             12138..13568
                     /locus_tag="VLE22_24920"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17715.1"
                     /translation="MALQAGTRAMIAATDHPPFARKLTKGIICGMICLASAAALRPET
                     ASKPQSELNQAALLYVQGMYEPAERIYRQELAKMEQTVGPNDPSLVNVLTQLGTVYTS
                     LGDFHQAEAFHRRALAIAEKSPGQVQPQLAIVLSNLAAACYHLGNHAEAESLAKRALA
                     IQEKILGPAHTDIINTLNNVAALYHSQGRLAEAEALYRRSLAIAETTLGPEHPTVALI
                     LGNLAELYARRSRYVKAEPLYRRSIAISEKAWGPEHPTLVPGLHNLANLYRDLEYYER
                     AVPLYERAIAILERTAGPEHPDLAGVLHSMANLYARQNDLPKVESLLRRSLDIWEKTL
                     GFQHPNVAAATHDLGRLYLVQHKYGKAQPLLERALAMREAALGGDNPSLAPALRELAD
                     LYVAKRQYGSAERLYTRLLAIQEKTLGPRHPDYAANLQAYASVLRKLNRKSYAEQVNA
                     SARAILSRDAREVAHAALRTTSDDRR"
     gene            13664..14266
                     /locus_tag="VLE22_24925"
     CDS             13664..14266
                     /locus_tag="VLE22_24925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB17716.1"
                     /translation="MALVRRIRAGDQTAFRELVEQCQSKVFRVIYGILHNRDDAEDIA
                     QEVFTKVYLSIGTFDGRSSVLTWICRIAVNECYSWLRKRRVAVVCDGDSTGGSISLQA
                     ADVRTAAERDLIQRDLVNKALARIPEDDRFLLLWREVEGFSVTQLTEMIGSNENTVKV
                     KLFRARRKLAKAVTRLSTSRCTHRSQKPVGGKVQLEEVER"
     gene            14263..14787
                     /locus_tag="VLE22_24930"
     CDS             14263..14787
                     /locus_tag="VLE22_24930"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSB17717.1"
                     /translation="MKLSILFLVVLLAAAGSAAPKSMPERGRLEKEVRHELVMLPFYG
                     VFDNFAFKVEGDKITLLGQVTRPSLKRDAEAVVKQIEGVGSLDNRIEVLPPSPNDDRI
                     RLAAYRAIYGHSALNRYALMAVPSIHLVVKNGDITLEGAVATEFDKNIANTQANSVPG
                     VFSVTNNLRVDEGR"
     gene            14790..15392
                     /locus_tag="VLE22_24935"
     CDS             14790..15392
                     /locus_tag="VLE22_24935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17718.1"
                     /translation="MRATAPLFLVAFLSMAATPARQVVHQRAQQTTELARELVLERID
                     TIERNGQCRQEFGKEMIDLDRVRMTVRRIRFYNAEGPEGDLRFSEVVGRPASPDSRLR
                     DLSRGLSADAFVLGYPDNRGYIRTRNVVLSRGYFEQGDPREGTWRLRSREEKQALLLH
                     EALHIALDKDDDDLSQRELCPLRLLAFCPRAPAIVVAGSE"
BASE COUNT         3063 a         4573 c         4680 g         3082 t
ORIGIN      
        1 catcggcgta tttgaccgaa gtgctccgct cccaactgga ggacacatcg agcaggcgga
       61 cggtacgggc tggatgggga tgtactgtct gaatctcctc gcgatcgcga tggagctggc
      121 tcgcgaggaa tcgtgttacg aggacgtggc cagcaagttc tgggagcatt tcctctatat
      181 cgccaacgcg atcaaccatc tcggagaaga cggcgccggc atgtgggacg accaggacgg
      241 cttcttttac gatgtgctgc acatgcccga cggccgtcac tcgcctctga gggtccgctc
      301 gatggtcggg ctgattccac tgttcgccgt agaaacgctt gaaccggaga tcaccggcaa
      361 gctgaaaggc ttcacgcggc gcttggagtg gttcatcgaa aaccgcaaag atctgacgcg
      421 caatgtcgcg tgcatgcggt cgcccggaga gggtgagcgg aggctgctat cgatcgtcga
      481 ccgcgatcag cttcggcgcg tgctgagagt catgctcgac gagcgggagt tcctgtctcc
      541 gttcggcatt cgtgcgttgt ccagaatcca ccgcgacgat ccgtacgtat tcgctgtcaa
      601 cggcgcggag taccgcgtgc agtacgagcc cggggagtcc accaccggcc tgttcggtgg
      661 gaactcaaac tggcggggac cgatctggtt tccggtcaac tatctgatta tcgagtccct
      721 ccagaagttc catcactatc tgggcgatga cttcaaggtc gagtgcccca cggggtcggg
      781 acagttgatg acgctctggg aggtggcggc ggagatctct cgccgcctca cacgcacgtt
      841 cctgcgcggt ccggagggac ggcggcccgt ccacggctcg aatgaagtct tccacaacga
      901 tccgcactgg cgcgacctcg tgcttttcca tgaatacttc catggggacg acggatccgg
      961 agttggcgcc agccatcaga cggggtggac cggcctcgtg ggcaaactca tccagcagag
     1021 cggagagtga gggcgccgtt gaggggcaag atccgacttc tctctctgct ctgtgtgcgt
     1081 gaaatgccct cctcttgcag cacggaataa tgcagcagtg gatccggatt cataggcgtg
     1141 atgcaaaacg tcttcagaat cgtggctggc tgtttcttgg ccttcgcatg ccaggcgcaa
     1201 acgctttcct tctcgcttct cggagcgcaa gacccacaac cttccgcgcg cttcgatggc
     1261 accatcgctt atgatccggc cggccgccag atcttcctct tcggcggcca agacattgcg
     1321 ccgaaaaacg acctctgggt gtactcgctc gcgcggaggc ggtggaccga ggttcaggtt
     1381 tcgagtgcgc gaccgcctgc tcgattcggc cataccctta tctttgatcc aattcgacgg
     1441 cgactcgtcg cttttggcgg acaagcgggc ggtttcttca gcgacgtctg ggccttcgac
     1501 atcgtgcgag gcttgtggga gcagttgagc gcgaatgaga cggggcccag ccgccgctat
     1561 ggtcactcgg ccatctacga tgcagctcgc gaccgtatgg tcatctcgca tgggttcacc
     1621 gacgccggcc gcttcgacga cacttgggcc ttcgagttca ccagtggctc ttggcgcaac
     1681 atcagccctt ccggtacccg cccgctccgc cgctgccttc atcatgcggt gtacgactcc
     1741 gcaaacaacc agatgctgct ctacggaggc tgttccagcc cggtcggacc atgtccgttg
     1801 ggagatcttt ggtctttcga tctcaccgcc gggcgttgga ccgaacgcac tccacaatcg
     1861 agtcctcccg cacgagagca ctatggcatg gcgttcgacg ccgtccgtgg cctcctggtg
     1921 gtgtttggcg gcaacggaaa tgggctgctc aacgacacgt ggcgctacga tccccgctcc
     1981 ggcacatggc agcagcctcc ggtggaagga cccgtgccga gtccgcggcg acggcacgag
     2041 acggcgtatg ccaccgaccg gggcacgatc ttctttttcg gcggcgtaac cgcctccggg
     2101 ttgacgaacg agctctggat ggttggcccg ggattcgcac atgaccggcc cgaagttccc
     2161 cagaacgggg tagtcgacgc tttcgacggc gagggaggtg gcgtagctcc gggtgagatc
     2221 gtgtctatct tcgggagcgg gttgggccca gtgaccggtg tcgctctaga gtttgaccct
     2281 caaaccggcc aacttccgac gtccgggccg ggcgtgtccg taacctggaa cggcatgcct
     2341 gctccgttgt atttcgtcag atccgatcag ctcaacgtgc aggtccccta cgaactcgca
     2401 gcagccaccg aagcgagcct ggtggtcacg gtcaacggtc agtcgagtga tccgattact
     2461 ctcccggttg tcccgacgcg gcctggactc ttcccaggca tttggaacca ggacggcagc
     2521 gtgaactcaa cggacaaccc ggctccggct ggcagcatcg tcgtgctcta cgcgacaggt
     2581 caaggcgtca ccgatccgcc aagccggaca ggggcctatc cggtcggact ctaccctgcg
     2641 ccgtccgcag ctatgtcatt aagcattggt gggactgatg cggagatcct ttttcgtggc
     2701 caggcgccgg gcactgccgg tgtgatgcag atcaacgcca gagtaccgga agggttgaca
     2761 gccgcgtcgc cggctgctgt ggttctgacg gtgggcgcgg caagcagccg ccccggcgtc
     2821 ctactcagcg ttcggtgaag tggatcaccc gatgctcggg aatgattccc gctctctcgg
     2881 ggattacaga gtgagcgggc aacaccgctc aggcacccgt aggaggatca gccatgaaaa
     2941 tcgtaaaggg tcgacttgca ctcgctgttt tgctcggcat cgcatcaggt gcgccggcgc
     3001 aggttacgga caagaaggca ctgacgctgg atggcgcgcg gaagatcatc gccgctgcgg
     3061 ttgcagaagc gaagcgggtc gatgctccag gcgcggtgat cgctgtcgtg gatgacggcg
     3121 gtaatctgat ggcactggag cggcttgaca ataccttcgc cgccggcgcc aatatctcga
     3181 tcggcaaggc gcggacggcg gcgttgttca agcgcccaac gaaagtcttc gaggacatca
     3241 tcgggaaggg ccgtacgtct atggtcgccc tcaatgactt cacgccgctg cagggcggcg
     3301 tgcccatcgt ttcggaagga caggttctcg gaggagtcgg tgtgagtgga gcagccagcg
     3361 cccagcagga cgagcaactc gccgtggcag gcgcggaggc ggccaagagc ttcgccggcg
     3421 ccgccaatgg cgctcaatcg tctgcggtta gctactttaa ccacggaacg gtggaggccg
     3481 catttgccaa gggcgcggtg ctgttcgacg gaagcgacaa gtacatggtc cacgccagcc
     3541 gccgggacaa gccaggtcag gccgaagtcc atacaaagga cgcggatatc atctacgtac
     3601 tcgacggaaa cgccacgttc gtcacaggcg gcactgccgt cgacgctaag acgactgccg
     3661 ccgacgaaat ccgcggcact gagatcgctg gcggcgacac ccgcgaaatc agcaagggtg
     3721 acgtcatcat tgtcccggcc ggcactcctc actggttcaa ggaagtgtcg aatccgttcc
     3781 tctactacgt cgtgaaggca cgctagtcga gcgcacacga ggccgccaat gaagaagaac
     3841 accgttcgag ttgtttttgc gttattgccc tttgtcgtcg ttgcctccgc agcgaagatc
     3901 cgacccgagg cgcccagcgg ctcaccgagc gccacaatcg accttgcagc ggatgaggga
     3961 gtgcggctgg tgaacggcca gtggcgctac agcgacacta agatcataga ggtggaattc
     4021 cgcggtcccg gccccgatgg acagccgacg ggcgcccccg ttaagactta tgactacact
     4081 cctcacgcgg gcactgcgga cttcgacgac tctcagtggg aaaccatcaa agcagcaaac
     4141 ctgaacgtgc gacgctcgac gggccggatt tgcttcaact ggtaccggat caacatcacg
     4201 gttccggagc gaatcggcgg ctttaatccc gccggcgcaa cggtcgtatt cgagacctca
     4261 ctcgacgatt atgccgaggt gtgggtggac ggcgaactgt cgcgcgcgct cggacaggtg
     4321 ggcgggtcgg tagttggtgg ctggaacgct ccgaatcgtc tagtcattgg acgcggcgtc
     4381 acgccaggac aaaagattca gcttgctgtc ttcggcatca acggtccact ttccaatccg
     4441 ccgacgaact ttatttggat gcggtatgcc cggctggaat tttacgaggc tagcccggag
     4501 cccgttgcca tcactccccg cgaagtgaac gtggaagtcg tccggcttga tcccgcgatg
     4561 gacaccatcg tcgggccgaa tccgaagatc ttcaaactcg ccgaagggtt ccggttcacc
     4621 gagggtcctg tctggattag cgacggcggc tacctgctct ttagcgaccc gaacagcaac
     4681 acgatttaca agtacactcc aggcagcgac aacagcggtg agctttctgt cttccaccat
     4741 cccagtggct actccggcga cgatatcgcg gagtaccgcc aaccgggatc gaacggtttg
     4801 acactcgatc cacagggccg cctgaccatc aatgagcacg gcaaccgccg ggtgactcga
     4861 ttcgagaagg acggaactct gacagtgctt gccgccagct acgaaggcaa gcgcctcaac
     4921 agtccgaacg acctcgtgta ccgttctgac ggtacccttt actttaccga tccgcccttt
     4981 ggcctgccga agttcttcga cgatccccga aaggaactgc cctacagcgg tgttttttcg
     5041 ctctataagg ggaagcttcg actcgtcagc acggacttca gcgggccgaa cggcatagcc
     5101 ttctctccgg atgagaaata tctgtacgtc gccaattggg atgacaagaa gaaggtcgtc
     5161 atgcgatacg aggcgaatgc cgacggtact ctttccaacg gcaggctctt ctttgacatg
     5221 acgaacgcca agggcgaaga tgcgctggac gggataaaag tggacaagca aggcaacctt
     5281 tacgtgtccg gtcccggtgg gctttgggtt ctttctgccg agggcaaaca cctcggcaca
     5341 atcattccac ccaagcaccc gcacaacctg gcgtggggtg acgaggacgg caagacgctc
     5401 tatttgtgtg cgcgcagcgg gctctatcgc atgcggctga acgtcattgg cgtgcggccc
     5461 tgatggcggt tttcactgag ggcgaggaga ttcggaatga gccacattca actcaagtgc
     5521 gcaccggcag cccgaacgcc gccgcttcgg accgaggctg ccggcgcatg gtcggcgagt
     5581 tgcgcgcgaa gcaactgccg gcccctgctt aggcgggaca ttactgtccc gatcggaata
     5641 cctaggctct cctgaatctc cttgtacgag aactcatgca cgtccgcgag catgacgact
     5701 tccgcaaact gctggggaag actccggaag gccgacagaa tcgcctggtc ggtgagattg
     5761 tcgggcacag gagcggcgta gggtgtggtg tcctcaaatg tctttggttc gcacaaaacg
     5821 acacggctca gccattttcg gcgctgatgt gaaatcacat ggaagaggat gccaaagagc
     5881 caggcgcggc agttcgttcc gggcgtgaac cgatcgaagg atttccacgc ctgcaagtac
     5941 gcttcctgca ccgcatcttc ggcttccgcc gcgctaccca ggatcgaaga cgccgtccgg
     6001 aacaggtctc ttgtgtgcgg caaagcgatg tcttcgaaac tcgccaggcg ttcggatgtg
     6061 tccgcttgag tcgtcatacg gcacccctct caagtgggga ctgaagtgtt cgttaggacc
     6121 cagagttctt caactgttcc accaggaaaa gtgggcagtt gatcaaggtg gcctttcggc
     6181 ttaccaggtc gcggagagcg gccacaccgt cccggtcgac aaacgacaca tcgccgagat
     6241 cgagagtgag ccggtagcct ttgcccagaa agcgttcgca gaaagttctg agttccatca
     6301 cccagggacc gatcacacgc ccctctatca gaaacgtgac ggaatcgctc cccatggcgc
     6361 cctctgagat cctcaacatc ttcagttccc gttcttgtag caactcagcg cacgccggat
     6421 gccaatggta gaccggtcag atttgaatta agtccttgcg aatcagtacg tgtgcgccgt
     6481 cgtggccacg aaatgtcgtg gggggcggga aggcggcccc ctggacgcta cgatatagcg
     6541 tggcggacac gctgaatccc aagtttcttc atccggctgc gtagggtgtt ggggtggagg
     6601 ttgagcaatt tcgctgcgcc tcccggcccc tcgatgaccc atccggcctt ctcgagcaca
     6661 gccagaatgt gccggcgttc tacttggtcg atggcggttg gctgctctgc ctgtatagga
     6721 tcagccgagg ttggctctgc tgtgcggatg acagaagagc tggcggcctg gttcgcggca
     6781 aaggggggca aatggtcgcg ctccaatgcc aggaccggac ctggggaaag gaccaccgca
     6841 cgctcgatca gattctgaag ctcgcggatg ttgcccggcc aggagtagtt caccagttga
     6901 tccatggcgt cctgcgagat gccctctact gcttttccga acctcttggc gaagcgagac
     6961 acgaagaaca tcaccagttg cggtatgtcc gtccgcctct ctcgcagagg gggcacctcc
     7021 aacggaaaaa cattcaagcg gtaaaaaagg tcggaacgga aacggcctgc gcgaactgct
     7081 tcagccaagt cgcggtttgt ggccgcgatg atccgtacat cgacacgctg cgtccggctg
     7141 ctgccgacag gttcgaattc cccctcctga agaacccgca gtagtttcac ctgtgtctca
     7201 ggtggcagct cgccgacttc atcgaggaac agggtgccac cgtctgccag ctcgaagcgc
     7261 ccagtgcggc gctcgagggc gccggtaaag gcgcctttgg tgtgtccgaa aagttcgctt
     7321 tctaccaagc ccgccgagat cgcgccacag ttcaccttga ccaacgggcg atcgcggcgg
     7381 cggctccggt tgtgcaccgc acgggcaacc aactccttgc cggtccccgt ttccccgcag
     7441 atcaatacgg cagagtcggc cggcgctact ttctccagct tttggagcag ctcgagcaga
     7501 gccggactgt ttccgatgat ctcttcgaaa ttgtgctccc tgcgaatctc ctcctggaga
     7561 taaacgttct cggcctccag ccttttcttt agcgtctcca cctcggcaag cgccgatcga
     7621 agttcctctt ctgcctggcg ccgtcgtgcg acctcttgtt tcatctctcc gtaaattcgt
     7681 gcgttttcca gagagatcgc cgcctgtgac gacaggatct ggatgactcg gatgtggtcg
     7741 gctttaaaag catccgcagc cagactgttt tcgaggtaga gaatgccgcc gagcttccct
     7801 tggtgcacta cggggacgca caggattgac ctaggtttgg ctgacctgac gtaggggtca
     7861 tcggaaaagc gctcatcctt tagtgcgtcg ccgatcacca cgctttcgcc ggtctttcgg
     7921 ctgtatcgca caacggactg gcacaggttc ggactttcgt ccagccgcac ggactggagc
     7981 acacgaacgt cctccctgtc cacagttccc tcggccgcga cgtgcagatt cccgtcattc
     8041 tcgtccagga aaacaacccg ttgtgcccca gcgttctcga gcgtaattcg caacagcctg
     8101 cggagaagat cctcgagcac gatttctcca gcaatggccc gggccgcctt gatcacggaa
     8161 gcgatgtcca cggagacgga atccgtgacg ccggtttccg gggcaagtcc acccttttct
     8221 tcgagatcgc gcacctttgc ggcagcgccc cagcgttcgt aaacgcgcga ggcttccctc
     8281 agataaagag ccgcgatggc ctcgtttccc cgctcgcgcc aaaaacggcc atagagttcg
     8341 tttgccaggg cctcattcca gaccctgttt gcggcccgtg cactgcggat ggcttcctcg
     8401 tacgacgcca tcgcctccca gccgtgcccg gatatgcgat cgatttcagc gtgcaggagc
     8461 agtgagtaac aaccgaagtt ctccgcgcag ttctcggcca acacaccgag ggagtcccgt
     8521 agggcagtca gttcggtaag gtacgtaccg cgctgctcct ccgtggcgtc gcgatacaac
     8581 gccgccagcg tgaggccgta aaagaaatcc aggagaaccg gccagatcgt gccgctcagg
     8641 tgctgcacca cctgacgggc cttttgggca gcttcgaaag cccgatcata ggctccaaat
     8701 gtcacataaa gagagagctt cgctacatgg tagaaggtca gagagaaggg atgtgtcccg
     8761 tacgtttcca gatacgtctc ttcctggaaa ctgtcgtccg atagtgagag tctatccgcg
     8821 gttctccctt ggagggcccg cgcccagttc ataatgacgg tctgcggagg aactaggctg
     8881 ctcatcttga tcttctcgag caccgcaatg ctcgccaagc agtcgcttac aaagcgttcc
     8941 aggtcccggt tagtcaacag cgctgcccag gtttcagtga gcgctccgta acccgcgtaa
     9001 gtgaagtcac ctgtttccag gccggcctgg cacgcttcgc gcgcgtgctg aattgaggtt
     9061 tcgagcggcc gccgccagag actaacgtgc gcttggaact gctggtgaac tttggcacgc
     9121 ctcttcttgt cattgaagcg ctcattaacg gctagagcca gcctccccca ctcatatgcg
     9181 gattggtagt cgcccctgac agggccgacg gtgatggcat gggtgacgta gccgtacgcc
     9241 gattcctccg tgtttccgtg ggccagcgag agacgtacca tcctggccga aatcaggcgt
     9301 gtgagcgcct ggctgcctac aaggtaggcg gaggcccaga gcgccgtcag taggttcatg
     9361 accatccgca actcaaggtc ctgcatgggc ggaaggtcaa cgaggctatg aatggttcgg
     9421 ccttcgagga gtgcttggat gtgagagagt tcaacttcca gcgcggcctg ctccccttca
     9481 gtcgaactcg gaaaggccac gccaaacagc gctaacccct ctcggccgta ctggacagca
     9541 gcggcgtagc gagccatgtt ctcgcactgg acgatcctca gaagcgagat ccgggctttc
     9601 tcgagattgg tccgcgccct tgtcaccaac cggtcgaagc aagtttctgc ttcagggaat
     9661 cgtcccgtga ggtattcgca ttccgcttct tcaatgtgaa gctcccacat caggttgtag
     9721 tcggaattcc aacgctcctc gctaagcaga tggagaccgg cctgcagata gatcagcgcg
     9781 gattggtaag ccgcctggga cttcgcttta cgtccggcgt tgagattgag caatgccagg
     9841 ttcacacgct cgactgcgtc atcgatcaag ttactgccgt ggttgaaatg ttgggcaata
     9901 tcaaagagct tctcctcact ggtgccggga tcccatcggg cgagaaggaa gcggcctaga
     9961 gtgaggtgca cctgccgcct ttgcaattcc ggaatcatcg cataggcggc ttgctgcact
    10021 cgatcgtgca gaaacgtgta accaccgcct tcgtcatcgg ccggcagaac gagtcctttc
    10081 tgcagcgcct cgcggatgtc acactccgca tcgtgggttc gctgttcgct gatctctgcg
    10141 agcgtgccgg cgtcaaactg gctgccgatg caggcggcga atgtgagtgt atgttgggcc
    10201 tttggggaca agcgctggat tctacggctc atgaggtcca cgacgttgtc ggtgatggcg
    10261 gcggccgcaa tcgcctccac gctgaaggtc cagcggccct gcgcatggtc gaacgtgaca
    10321 agaccctctt gcctgagtgt tttcaagaac tgaatcgcga agaatgggtt tccatccgtc
    10381 ttctgtaaaa ttactctggc cagcggctcg acatccgtta actgcccgtg cagggtgtcc
    10441 ctgaccagaa gtgtcaattc ggaaagttgc agcggcccga gcacgatgtg actaagtgga
    10501 gcgccggccg atttcagagc tctcacggtc ctcaccagcg gatgtcgtac atcgacttcg
    10561 ttgtctcgat aggcgccgat caacagcaga aagcggatgt cgtggctcgt gagcagcggc
    10621 tgaagcagac cgagtgtagc cgcatcggcc cactgtaagt cgtcaaggaa gattaccagg
    10681 ggatgctctc gacgcgccag tgcacccaca aagctctgga acacgtaacg gaaacggttc
    10741 tgggcttccg ccggaccgag cgggggtggc tgctgttgct tgcccagaac cagctcgatc
    10801 tccgggatga cctccgccag cacgcctccg cccccgccga gagcgttcga gagctgagtg
    10861 cgccagcggg cgagctgctc ctcggtctcc gtaagcagtt gccgaatgag atctcgaaag
    10921 ccctggatca acgcgccata tgggatgttt cttacgatct gatcgaactt gccggcaatg
    10981 aagtacccgc gctggcgaac gatgggcttg tacagctctt ggataaggga agtcttcccg
    11041 atcccggagt agccggacac tagcgccatt gccgtgccgc cttctccggc gcggtcaaac
    11101 gcctcgatca gatcgccgac ctcgcggtct cttccgtaga gcttctgcgg aatcagaaac
    11161 cgatcggaga catctcgctg ccctgggaca aacggtgcga tcgaccgcct ttcggtccac
    11221 tcctgttggc agatctgcaa atcctggctc agcccgaggg cgctctggta tcggtcctcg
    11281 gcggtcttcc cgaggagctt cataacgatt tgcgatatct gttctggtac ctctggaagg
    11341 atttcggcgg gtggtactgg agtcttagcg atgtgcgaat gaatgagttc gagggcatcg
    11401 ccggatttga acggcagagt tccggtcagg agctcgtaga aagtcacccc cagggagtag
    11461 aagtccgtcc ggtagtccgt ggcacgattc attcggcccg tctgctccgg cgatgcgtag
    11521 gctagcgccc ctccgaggag tatggccggc gacgatcgga tttggcggga gcacccggat
    11581 gcaagagtga agtcgatcaa ctggaggacg ctgaaatcgg gcggcgccca gatggcagag
    11641 ggagtcagac tcccgtggat gatgtcctgg cggtgcagct cggccaggat ctcacagagc
    11701 gtacgggcga tttggaggaa cgagttgagg tctggccgac ccgtctcgat caggcgcctg
    11761 agtgggatga gacctcggtc ttcaagcacc aagcagggcc cgccttcacg ttgcacgagc
    11821 tcataggcgc gaggcacgcc ggctaccgag agcccgcaca gcagttcaaa ctcgtgctcg
    11881 agggacactg cgtcggctac ggtgcagggg acccgagctg cggctttcag caggactggc
    11941 ctcttgtcct ccgtgcccct gccccgactg atcacgtagc gctcgtcgcg taacacttct
    12001 tgcgcaacgt cgtagccacg aatctccata gctcggcagc cctcgtgtca gcagtttctt
    12061 cattgtagac cactgagccc gccgcggtag gtatcgaacg gaatatctcc atcgctcttc
    12121 gggatgtatc gagtgttatg gcacttcagg cagggacgag ggcgatgatt gcagccaccg
    12181 accatccgcc ctttgcgaga aagctaacta agggcattat ctgcggcatg atctgtctgg
    12241 cgagcgcggc tgctttgcgg ccggaaacgg cgagcaagcc ccagtcggag ctgaaccagg
    12301 cggcgctcct gtacgtgcag gggatgtacg agccggctga aaggatctac cggcaggaac
    12361 tggccaaaat ggagcaaacc gtcgggccga acgacccaag ccttgtcaac gttctcaccc
    12421 aactgggcac ggtctataca agtctgggcg atttccacca ggccgaggcc ttccatcgga
    12481 gggctttagc tattgccgag aagtcgccgg gccaggtgca accgcagttg gccatagtgt
    12541 taagtaactt ggctgccgcc tgctatcacc tgggcaacca cgctgaagcc gagtcgctcg
    12601 ccaagagggc cctcgccatc caggagaaga ttttgggacc cgcgcacacc gacatcataa
    12661 acacattgaa caacgttgcg gcactctacc attcgcaagg gagattggct gaagcggagg
    12721 ctctttaccg gcgatcactc gcaatcgccg aaaccacgct tggaccggag caccccacag
    12781 tcgccttgat tcttggcaac ctggccgaac tctacgcccg gcgcagccgt tatgttaagg
    12841 ccgagcctct ctaccggcgg tcaattgcga tcagtgagaa agcctggggg ccggaacacc
    12901 caactcttgt gcctggcctg cacaatctgg caaacctcta tcgggacctc gagtactacg
    12961 agagagctgt tccgctctac gagcgagcga tagcgattct cgagcggact gccgggcccg
    13021 agcacccgga tttggccggt gtgctgcaca gcatggctaa cctttacgcg cgccagaacg
    13081 atctcccaaa ggtcgagagt ttattgaggc gctcactgga catctgggag aagacgctgg
    13141 gctttcaaca tccaaatgta gctgctgcca cgcatgacct gggacgactt tacttggttc
    13201 agcacaaata cgggaaagcc caaccgctgc ttgaacgagc gctggcgatg cgcgaagcgg
    13261 cgctgggtgg ggacaatcca agtctggcgc cggccctccg cgagttggcg gacctgtacg
    13321 tcgcgaaacg ccagtacggg agcgcagagc gcctctatac tcggttgctc gccattcagg
    13381 agaagaccct cggcccaagg catcctgact atgcagcgaa tctgcaagca tacgcgtccg
    13441 tactacgcaa gttgaaccga aagagctacg ccgaacaagt gaacgcgagc gccagggcta
    13501 ttctgtcgcg cgacgccaga gaggtcgccc acgcagctct tcgtacgacc tcggatgatc
    13561 ggaggtaagt gacatgcagg ctttgatact cgagacagaa cactttctaa gttgtccttt
    13621 gaaaccctgc agggtggcgg ccggtgcggg taaggttggt gacatggcgc tcgtgcggcg
    13681 catccgggcc ggcgaccaga cggcctttcg cgaacttgtc gagcagtgcc aatcgaaggt
    13741 ctttcgggtg atctacggaa tacttcacaa ccgggacgat gcggaagaca tcgcccagga
    13801 ggtcttcacg aaagtatatc tttccattgg tacttttgac ggccgcagct cggtgctgac
    13861 ctggatctgt cggatcgcgg ttaacgagtg ttactcgtgg ttgcgaaaaa gacgggtcgc
    13921 ggtcgtctgc gatggggact ccacgggagg ctccatcagc ctgcaagcgg cggacgtgcg
    13981 cacggcggct gagcgtgatc tcatccagag ggacctggta aacaaggcgc tggcgcggat
    14041 ccctgaagac gaccggtttc tgctcctctg gagggaagtc gaaggatttt cggtgacgca
    14101 attgactgag atgatcggtt cgaatgagaa cactgtcaag gtgaagcttt tccgagcccg
    14161 ccgcaagctc gcaaaagctg taactcgttt gtcgacatcc aggtgcacac atcgatctca
    14221 aaagccggta ggcggaaaag ttcagttgga ggaggtggag aggtgaagct ttcgattctg
    14281 ttcttggtgg ttttgttggc tgcggctggg tccgctgcgc cgaagtcaat gcccgaacgt
    14341 gggcgattgg agaaagaggt acgtcacgaa ctagtgatgc ttccgttcta tggggtgttc
    14401 gacaatttcg ccttcaaggt cgagggggac aagatcacgc tgctgggcca ggtcacgcgt
    14461 ccgtccctaa aaagggatgc ggaggcggtc gtcaagcaga tcgagggcgt agggagcctc
    14521 gataatcgga tcgaggtgtt gccgccttcc cccaacgacg accgtatccg actggccgct
    14581 taccgcgcaa tctacggtca cagcgcgcta aatcgttatg cgctcatggc tgtgccatcg
    14641 atccacctcg tcgtaaagaa cggagacatc actctcgaag gagccgtggc tacggagttc
    14701 gacaagaaca tcgccaatac ccaggccaat tccgttccgg gtgttttctc ggtaacgaac
    14761 aacttgcgtg tggacgaggg gaggtaggcg tgcgcgcgac tgctccgttg ttcctcgtgg
    14821 ctttcctctc gatggcggcg actcctgccc gtcaggtggt gcatcagcga gcccagcaga
    14881 ccaccgagct cgcccgggaa ctcgtgctcg aaaggatcga cacgatagag aggaacgggc
    14941 agtgcaggca ggagttcgga aaggaaatga tagacctcga tcgcgtccgg atgacagtcc
    15001 gccgcattcg cttctacaac gccgaaggcc ctgagggtga cttgcgtttc agcgaggtgg
    15061 tgggcaggcc cgcttcgccg gacagccggc tgcgggacct ctcgcgcggc ttgtccgcgg
    15121 acgcgttcgt cctcggctat ccggacaacc gggggtatat tcgcacgcgg aacgtggtcc
    15181 tgagccgcgg gtacttcgag caaggcgatc cgcgtgaagg aacttggcgt ctcaggagtc
    15241 gggaagagaa gcaagcgctg ctgctccacg aggcgctcca catcgccctc gataaagacg
    15301 acgatgacct ctcgcaacgc gaactctgcc cgttgcgcct gctggcgttc tgtccccgtg
    15361 cgccggcaat cgtcgtcgcc ggctcggagt agatgccg
//
LOCUS       DATIFQ010000496         8656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2663067, whole genome shotgun sequence.
ACCESSION   DATIFQ010000496 DATIFQ010000000
VERSION     DATIFQ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2663067"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VLE22_24940"
     CDS             <1..1572
                     /locus_tag="VLE22_24940"
                     /inference="COORDINATES: protein motif:HMM:NF021594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HSB17719.1"
                     /translation="KKNRLMAPQLRRWTSTVELDLRGKLYRISRNTKNPRRVILEGDF
                     KDWPIKPKRDNETGEFVVSTAQGTDLLGWLMFDLAPQAKADSFEPTFRSLFSYFVRRG
                     RDAFSTPFEHFRKQADWDKQVNNAFLLGLSWEYPSKLQKLKDQEKAVSQLRSAIRAGT
                     FSDLLGTVGEMESQKVRLEELVARRGEELRTFRVHPQYQEYQEQANILQAAIRQLNQE
                     NTTDQAMLSFYEESIVQERPPEATKLAKLYQEAGVSWPDGVQRRLEDVQAFHDKVIVN
                     RRAFLGTETSQLRRAISQRTEELRKRSDERASLLSLLNTHGALAEYAEMQRLQNEEIA
                     KLSAVNQKIANLKRIDEIRSQTRIEREQLQILARSFYEERRVDRERAISLFNGFSQAL
                     YSRPGKLIVNVVGGGYSFDVDIERQDSQGVEQMKVFCYDLALATLWAAKRTSPGFLIH
                     DSTIFADVDERQRAHALELTAKTAGEQGFQYICCLNSDGIPNEDFSEGFSLQPFVRIE
                     LTDEGEKGGLLGIRY"
     gene            1841..2536
                     /locus_tag="VLE22_24945"
     CDS             1841..2536
                     /locus_tag="VLE22_24945"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HSB17720.1"
                     /translation="MPVRHQERHLTGRISWLRAAVLGANDGIVSTASLVLGVAAAHST
                     HSNILVAGVAGLVAGAMSMAAGEYVSVHSQADTEQAELARERVELKTDDESEHKELTA
                     IYIARGLDPSLAKQVAEQLMAHDAIGAHARDELGMSETLSARPIQAALVSAGSFAVGA
                     ALPLLVTAIAPEARLIPLVSGTSLAFLALLGGVAARVGGAGVTVGAIRVLFWGALAMA
                     LTAGVGALFGTVA"
     gene            2765..3031
                     /locus_tag="VLE22_24950"
     CDS             2765..3031
                     /locus_tag="VLE22_24950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17721.1"
                     /translation="MMHRDQAELTTPQREAAMFYGLFLRGHAVDTLRRDIDVPKAVIR
                     KWLHAPHYEPGFRDALRRVYQYRKMVLAIFDELVLHEQRQPRLQ"
     gene            complement(3035..6118)
                     /locus_tag="VLE22_24955"
     CDS             complement(3035..6118)
                     /locus_tag="VLE22_24955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HSB17722.1"
                     /translation="MLNRIIDFHLRHRWFVLAGLAGLIGLGLYVMLQIPIDAFPDLTS
                     NQVVVITECPAMPPSEVEQLVTFPIETALMGIPRTQGIRSISKLGLSMITLVFDDSVN
                     TYFARQLVNERLQEVRARLPEGLEPTLGPVATAFGEVYQYTLEGKGYSSMDLKTLHDW
                     QIKNQLRTVPGVNEVNTWGGETRQYQVQVDPVSLQRYGLTLRDVFERIRENNANFGGG
                     YIEHASEQYTVLGLGRTQNMADLEQVVVLSRAGASVLLKEVAKISVEPMPRQGAVLRD
                     GKGESVSGMAIMLKGENGLRVIERVKAKLASLRLPEGVRIAPFYDQSTVIDGTIRTVK
                     KNLFEAGVLVILVLLLFLGNIKAALIVAAVIPLSMLVGFIGMRVFGVSANLMSLGAID
                     FGMIVDGAVVMMENAVRNLQRSPGDEHFDATERVRRAAHEVARPILFAVAIIIAVYMP
                     ILFLEGLEGRMFRPMAITVCSALIGALALALTVVPTAATIALRGGAKEHQERWFERLR
                     GWYTAVLEWSLEHRLLVVSVSVGVLVVTLGSLRYIGTEFMPRLDEGSLLVETRKLPGI
                     SLTESIQLSNRVEQVMVSFPEVTGVVTKMGRPDLATEAMGIYQGDVYVLLKPRDEWKR
                     FRTKEELIEAMAAKLESVPGVNYNFTQPMAMRLDEVVSGIKADVAVKIFGEDARVLEQ
                     LAERALRLVSNVPGAADAQMEIVSGVAELRVEVDRPALARYGLNVSDVREIVEAAVGG
                     RQVSEMIEGQRRFAIVLRLPERYRQDAVSLSDVTLSAPGGERVRLGQVARVSVTRGPE
                     VISREDGARRIVVQANVRGTDLGSFVAEARRRVEAGLPLPPGYSIDWGGQFENQARAM
                     RRLLVVVPAAVLIIFALLFATFDSVPQALLILLNVPFALVGGIGALWARGLNLNLSAS
                     VGFIALFGVAVLNGIVMVSYMNRLREDGMACRQAVIEGAQVRLRPVLMTALVASFGFL
                     PMAISTSTGAEVQRPLATVVIGGLITSTMLTLFLLPVLYPWFCKGAAREGETVRV"
     gene            complement(6126..7337)
                     /locus_tag="VLE22_24960"
     CDS             complement(6126..7337)
                     /locus_tag="VLE22_24960"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB17723.1"
                     /translation="MKAWTTAVLILILTGCSRQTPTAASEATSQAGTATGREVTLDEK
                     AQREGGIAVAAIEVQALPETLQAAGRMTVNENRTWRVGAITEGRIIRVFANAGDGVEE
                     GQILARMHSHDIHESRAEHRKAVAEVARLKNLESYALRVRDRAKRLLELKAASVEQVE
                     HAETELRNTQTLRANAEVELDRTRKHLTEFLEIPIEEPKEHHSGMQERDDDLIPIKAP
                     ATGTLLTRNVTPGTVVASSGDLFVVSDLSTLWMIALVNEEHLPALRAGMPVRVRVQAY
                     GTQAFPGRIGKLGEELDPTTRTVRVRVDVPNPRGLLRPEMYATAEIELGSSKPGLYVP
                     QEAIQEVNGQSAIFVRTAVDRFEARPVRAGTPVQNSVEIVRGLKPGEQVAIAGSYVLK
                     SQLMKASLAGE"
     gene            complement(7334..8551)
                     /locus_tag="VLE22_24965"
     CDS             complement(7334..8551)
                     /locus_tag="VLE22_24965"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSB17724.1"
                     /translation="MRRFTLLLLFALSAAAQTPLSLRDAVSQALSIHPLLSAGAGRVA
                     ASEGLRRQAALRPNPRMFFQVENLRGHGQPGFVYGRDADTYAYVSQVIETSGRRGLRT
                     EAASANVRRAELERELLARQIAARVKHAYWSAAGAQRVHELLVESMNTFRQVVEYHEL
                     RVREGAMAEADLLRVRVESERFAIAANSAALDAERARIQLLREMGRTEFPKVQLTDKL
                     DETGPFAGTVDAVRALEERLEVRAAKQAVEQAQTALRLERASARPNVDLLAGYKRSGG
                     FNTVLGGVQVDLPFANRNQGNIGAAEAEVRVAEASLAAVEATVKAEVRTAQSEYEIRR
                     RQLLDTLRTLREHAVESSKIALAAYREGGLDLLRLIDSERVRLDVEALYGRTLAEYRQ
                     SIVNLETALGVAP"
BASE COUNT         1653 a         2939 c         2448 g         1616 t
ORIGIN      
        1 aagaagaacc gcctgatggc acctcagctt cggagatgga cgtccaccgt cgagttggat
       61 cttcggggga agctgtatcg aatcagccga aacacaaaga accctcggag ggtgattctg
      121 gaaggtgatt tcaaagactg gcctatcaaa cccaagaggg acaacgagac cggagagttc
      181 gtcgtctcaa cggcgcaagg gaccgacctg ctgggctggc tgatgtttga cctcgctcct
      241 caggccaagg cggattcctt tgaaccaacc ttccgcagtc tgttttccta cttcgttcgc
      301 cggggacgtg atgctttttc gactcccttt gaacacttcc gtaaacaggc ggattgggac
      361 aagcaggtta acaatgcgtt cctcttgggt ctctcttggg agtacccatc aaagctccag
      421 aagttgaagg atcaggagaa ggctgtcagc caacttcgtt cggcgatcag agccggaacc
      481 ttttcggacc tgctggggac ggtgggcgag atggaatcgc aaaaggtccg gcttgaggaa
      541 ctcgttgcgc gccgtggcga ggaattgcgt accttccgag ttcatccgca gtatcaggaa
      601 taccaagaac aagctaacat actgcaggcc gccatccggc agcttaacca ggaaaacacg
      661 accgaccaag caatgctctc gttttacgag gagagcatcg ttcaggaacg cccacccgag
      721 gcgaccaaac tcgcaaagct ttatcaagaa gctggggtca gttggccgga cggggttcaa
      781 agacggcttg aggacgttca ggcgtttcac gacaaggtta tcgtgaatcg gcgcgccttc
      841 ctagggacag aaacctccca gctcaggcgc gcaatttcgc aaagaaccga agaacttcgc
      901 aaacggagcg atgagcgggc gagtctgctc tcacttctga atacgcatgg ggccttggcc
      961 gagtatgccg agatgcaacg gctacaaaac gaggaaatag ccaagcttag cgccgtgaat
     1021 caaaagatcg cgaatctcaa gcggatcgac gagatcaggt ctcagacgcg gatcgaaaga
     1081 gaacaactcc agatcttggc gcgctccttc tatgaggagc gacgtgttga ccgggagaga
     1141 gcaatttcgc tcttcaacgg gttttcgcaa gccctgtaca gcaggcccgg caagctgatc
     1201 gtcaatgttg tcgggggtgg gtacagcttt gatgtcgaca tcgaacgtca agacagccaa
     1261 ggcgtcgagc agatgaaggt cttttgctac gaccttgcgc tagcgactct ctgggcggca
     1321 aagcgtacta gtcccggatt cttgattcat gacagcacga tctttgcgga cgtggatgag
     1381 cgccagagag cccacgcact tgaactgaca gccaagactg cgggcgaaca gggatttcaa
     1441 tatatctgtt gccttaattc cgacggtatt cccaacgagg attttagcga aggcttctcg
     1501 cttcaaccgt ttgtgcgaat cgaacttacc gacgaaggcg agaaaggtgg attgctcgga
     1561 atccgttact gagtcgaata cggtgctgcc gtagaatacc tatcctatcc gtccagtgag
     1621 cccgatgttg ccttcgtctg gctgcgtatt cctcatgccc gcaaactctc ctcgcatcgt
     1681 agctgttccg ccgttcctga tccctcgagc cggcccgtcc acctcaaccc aaactgcccg
     1741 gggactggcg atcatgctag cccggaacga cagtgtccgc atccgcgatc ggcgattcct
     1801 gtaatctatt ctgcggttct cggattgagg aggctcaaga atgcctgtac gccaccagga
     1861 acgtcacctc acaggacgta tcagctggtt acgcgccgcg gtattaggcg cgaatgacgg
     1921 tatcgtttca acagcaagtc tggtcctcgg tgtcgcagcc gcgcattcga ctcacagcaa
     1981 catattggtc gccggggtcg ccggactggt cgcgggggct atgtcgatgg ctgccggtga
     2041 gtacgtatcc gtccactcac aggcagacac cgaacaggcc gaactcgcgc gtgagcgtgt
     2101 ggaactcaag acagacgacg aaagcgagca caaggaactg acggcgatct acatcgctcg
     2161 cgggctcgat ccgtcgcttg cgaagcaggt ggccgagcaa ctgatggctc atgacgcgat
     2221 cggcgcgcac gcccgggacg aacttggcat gtctgagacg ctcagtgcgc gtcctatcca
     2281 ggcagcgttg gtgtcagcag gcagcttcgc cgttggagcg gcccttcctc tcctggtcac
     2341 tgctatcgcc ccggaggcgc gtctgatccc cctggtctcc ggaacttcac tggcattcct
     2401 cgcgcttctg gggggcgtcg ctgcacgtgt gggcggggca ggcgtgacgg tgggtgcaat
     2461 acgcgtcctg ttctggggtg cactggctat ggccctcacc gcaggcgtcg gggcgctgtt
     2521 cggaacagtt gcgtgactgg ggccagccgt ggcgcgggag ggtccggtgc cgccaggctg
     2581 tgcatctgcc ggcaagaccg ccgacaccgc cgatcgcgcg aaccggcccc gccggggacg
     2641 ccttgggcag tttgcggttc gccgcatgtt gggacgtgct cggcaccacc ccccgttgcc
     2701 cgcgccggtg gaggctgtct atactacggt tttcgggggg ctccttcagc ggagcagggg
     2761 ttgcatgatg cacagggacc aagccgaact caccactccc cagcgggaag cggccatgtt
     2821 ctatgggctc ttcttgcgcg gtcatgccgt ggacacgctt cgccgcgaca tcgacgtccc
     2881 caaggccgtg atccggaaat ggctgcacgc cccacactac gaacccggct tccgcgacgc
     2941 ccttcggcgg gtctaccagt accgtaagat ggtcctcgcc atctttgacg agctggtgct
     3001 gcacgagcag cggcaacccc gcctccagta gccgctacac tcgcactgtt tcgccctctc
     3061 gcgcagctcc cttgcagaac catggataga gtaccggcaa caggaacagc gtcagcatcg
     3121 tcgacgtgat caacccgccg atcaccaccg tcgccagcgg ccgctgcacc tccgcgccgg
     3181 tcgaggtcga gatcgccatc ggcaggaaac cgaaactcgc caccagcgcc gtcattagca
     3241 ccggccgtag ccgtacctga gcgccttcga tcaccgcctg gcggcatgcc atgccgtctt
     3301 cccgcagccg gttcatgtaa ctcaccatca cgatgccgtt cagaaccgcc acgccgaaca
     3361 gcgcgatgaa gccgactgac gccgagaggt tcaggtttaa cccacgagcc cacaatgcac
     3421 cgatgccgcc cacaagcgcg aacggaacgt taagaagaat gagcagcgcc tgcggcaccg
     3481 agtcgaacgt cgcaaacagc agtgcgaaga tgatcagcac cgccgccggc accaccacca
     3541 acagccgccg catggcgcgc gcctggtttt caaattggcc gccccagtcg atcgagtacc
     3601 caggcggtaa cggcagtccc gcctccactc ttctccgcgc ctcggccacg aagctgccaa
     3661 ggtcggtgcc gcgtacattg gcctgcacga cgatccgccg cgcaccgtcc tcccgggaga
     3721 tcacctccgg tccgcgcgtc acgcttaccc tcgcaacctg gccgagccgc acgcgctccc
     3781 cgcccggcgc cgacagcgtc acgtcgctca gcgagaccgc gtcctgccgg taccgttccg
     3841 gcaggcgcag cacaatcgcg aagcgccgtt gcccctcgat catctccgac acctggcggc
     3901 cgccgacggc tgcctcgacg atttcccgca cgtcggagac gttcagcccg tagcgcgcca
     3961 aggccggacg gtccacctcc accctcagtt ccgccacccc cgagacgatc tccatctgtg
     4021 cgtcggcggc cccgggcacg tttgacacca gcctcagcgc ccgctcggcc agctgttcga
     4081 gcacccgcgc gtcttcgccg aaaatcttca cagcgacgtc cgccttgatg ccggagacga
     4141 cctcgtccag ccgcatcgcc atcggttgag tgaagttgta gttcactcca gggaccgatt
     4201 cgagtttcgc ggccatcgcc tcaatgagct cttccttcgt ccggaaccgc ttccattcat
     4261 cacgcggctt cagcagcacg tacacgtcgc cctggtaaat tcccatcgcc tcggtggcga
     4321 gatccgggcg acccatcttc gtaaccacac cggtcacctc cgggaagctg accatcacct
     4381 gctctacccg gttgctgagc tggatcgact ccgtcagcga gatgcccggc agcttgcgcg
     4441 tctccaccag cagtgaccct tcgtccagcc gcggcatgaa ctcggtcccg atgtaccgaa
     4501 gcgatccgag cgtgaccacc aggaccccga cggatacgga aaccacgagc aaccggtgct
     4561 ccagggacca ctcgagcacc gccgtgtacc acccgcgcag ccgctcgaac catcgctctt
     4621 ggtgttcctt cgcaccgccc cgcagcgcga tggtcgccgc cgtcggcacc actgtcaatg
     4681 ccagcgccag cgcgccgatc agcgccgaac ataccgtaat cgccatcggc cggaacatcc
     4741 gtccctcaag cccttctagg aacaggatcg gcatgtagac cgcgatgatg atcgccaccg
     4801 cgaacaggat cggccgtgca acctcgtgcg ccgcccggcg cacgcgctcc gtcgcgtcga
     4861 agtgctcgtc gcccggcgag cgctggaggt tgcggaccgc gttttccatc atcacgaccg
     4921 cgccatccac gatcattccg aagtcgatgg cgccaaggct catcaggttc gccgacacgc
     4981 cgaacaccct catccctatg aacccgacga gcatcgaaag cggaatcacc gccgccacaa
     5041 tcagtgccgc cttgatgttt ccgagaaaca gcaacagcac caggatcacc agaacgcccg
     5101 cttcgaacag gttcttctta accgttcgga tggtgccgtc aatcacggtc gactgatcat
     5161 aaaaaggcgc aatgcgcacg ccctccggca ggcgcagcga cgcgagcttc gccttcaccc
     5221 gctcgatcac ccggagccca ttctcgcctt tcagcatgat cgccatgccg gacaccgact
     5281 cgccttttcc gtctctcagc accgcgccct gcctcggcat cggctccacg gagatcttcg
     5341 ccacctcctt cagtagaacc gaggcgccgg cgcgagaaag cacgacgacc tgttccagat
     5401 cggccatgtt ctgcgttcgc cccagtccga gcaccgtgta ctgctctgag gcgtgctcga
     5461 tgtagccccc gccgaagttc gcgttgttct cgcggatccg ctcgaaaacg tcgcgcaacg
     5521 tgaggccata tctctggagg ctcaccgggt ccacctgcac ctggtactgc cgggtttcgc
     5581 cgccccaagt attgacctcg ttgacgccgg gcaccgtccg gagctggttc ttgatctgcc
     5641 agtcgtgcag cgtcttcaga tccatcgacg agtagccctt gccctccagc gtgtactgat
     5701 acacctcgcc gaaggcggtc gctaccggac cgagagtcgg ctccagtcct tccggtaatc
     5761 gcgcccgcac ctcctgcaac cgctcgttta ccagttgccg cgcgaaatac gtgttgaccg
     5821 agtcgtcgaa cacgagagta atcatggaaa ggccaagctt cgagatcgat cggatgccct
     5881 gggtccgcgg aatccccatc agcgctgttt cgatagggaa cgtcacgagc tgctcgacct
     5941 cggagggcgg catcgccggg cactcggtga tcaccaccac ctggttgctc gtgaggtcgg
     6001 gaaaggcgtc gatcggaatc tgcagcatca catacaagcc gaggccgatc agcccggcca
     6061 gccccgccag cacgaaccag cggtgccgaa ggtgaaaatc gatgatccgg ttgagcatgg
     6121 ccgcgttact ccccggcgag cgacgccttc atgagctgcg acttcagcac atagcttccg
     6181 gcaatcgcca cctgctcacc cggcttcagc cctctcacga tctcgaccga attctggacc
     6241 ggcgtccccg cgcggacagg acgcgcctcg aaacggtcca cggcggtgcg cacaaagatc
     6301 gcgctctggc cattcacctc ctggatcgcc tcctggggta cgtacagccc gggcttcgaa
     6361 ctccccagct cgatctccgc ggtggcgtac atctccggcc gcaacagtcc ccgcggattc
     6421 ggaacgtcta ccctgaccct gacggtgcgg gttgtcggat ccagttcctc gcccagtttg
     6481 ccgatccgcc ccggaaaagc ctgcgtgccg tacgcttgca cacgaactcg caccggcatg
     6541 ccggcccgta acgcaggcaa gtgttcctcg tttacaagag cgatcatcca gagcgtcgag
     6601 aggtcgctca ccacgaaaag gtccccgctc gaggcgacca ccgtccccgg cgtgacgttc
     6661 ctcgtgagca gcgtacccgt agccggcgcc ttgatcggga taaggtcgtc gtcgcgctcc
     6721 tgcatccccg agtggtgctc tttcggctcc tcgattggaa tctccagaaa ttcggtgagg
     6781 tgcttgcgag ttcgatcgag ttccacttcc gcgttcgccc gtagtgtttg cgtgtttcgg
     6841 agttccgtct cggcgtgctc cacctgttcg acagaagcag ccttcagctc aagcagcctc
     6901 ttcgcgcggt cccgaactcg aagggcgtac gactcgaggt tcttgagccg cgccacctcg
     6961 gccactgcct tacgatgttc agcgcgcgat tcgtggatat cgtggctatg catccgggcg
     7021 agaatctggc cttcctcgac cccgtctccc gcattcgcaa aaactcggat gattcgcccc
     7081 tcggtaattg cgccgacccg ccacgtgcgg ttctcgttca ctgtcatgcg gcccgccgcc
     7141 tgaagcgtct ccggaagcgc ctgcacctcg atcgcggcga cggcgattcc gccctcccgt
     7201 tgtgcctttt catcgagggt gacttcgcgt ccggtagccg tacccgcctg ggacgtcgcc
     7261 tcagacgccg ccgtcggcgt ctggcgcgag caccccgtca agatcaatat cagtacggcc
     7321 gtcgtccatg ccttcatggc gctaccccca acgctgtctc cagattcaca atactctgcc
     7381 ggtattcggc gagcgtccgg ccatagagtg cctccacgtc caagcggacg cgttccgagt
     7441 cgatcaggcg caacaagtcc agcccgcctt cccggtacgc cgccagagcg atcttggacg
     7501 attccactgc gtgctcccgc agcgtgcgca gtgtgtccag cagttgccgc cgtcgtattt
     7561 cgtattccga ctgcgccgtc cgcacttcgg ccttcaccgt cgcctcgacc gccgccagag
     7621 aggcttccgc caccctaacc tcggcttcgg cggcgccaat gttcccctgg ttccggttcg
     7681 caaacggaag gtcaacctgt acgcctccga gcacggtgtt gaatcccccg gaacgcttgt
     7741 aacccgccag gagatcgacg ttcggccgcg ctgatgcgcg ctccaaccgt aacgccgttt
     7801 gcgcctgctc caccgcctgc tttgcggccc gtacctccag ccgctcctcc aacgccctca
     7861 cggcgtcgac tgtccctgca aacgggcccg tctcgtcaag cttgtccgtc aactgtacct
     7921 tcggaaactc ggtccgcccc atctcacgca gaagctggat ccgggcgcgc tcagcgtcga
     7981 gcgccgccga gttcgcggca atcgcaaacc gctcgctttc gacgcggacg cgaaggaggt
     8041 cggcctccgc catcgccccc tctcgcactc tcagttcgtg atactccacc acctgccgga
     8101 acgtattcat cgactcaacg agcagttcat ggactcgctg tgcaccagcc gcgctccaat
     8161 aagcatgctt aacgcgcgca gcgatctgcc gggcaagcaa ttcccgttcg agttcagcgc
     8221 gccggacgtt ggccgacgcg gcctccgtcc gcagtccgcg cctccccgag gtctcgatca
     8281 cttgcgagac gtacgcatag gtatcggcgt cgcgcccgta aacgaatccc ggttgcccgt
     8341 ggccgcgcag gttctcgacc tggaaaaaca tcctcggatt tggccgcagc gccgcctgcc
     8401 gccgcagtcc ttccgatgcc gccacacgcc cggcgcccgc ggagagcagc ggatgaatcg
     8461 aaagggcttg cgataccgcg tccctcagcg agagcggcgt ttgtgccgca gcagaaaggg
     8521 caaaaagcag aaggagagtg aaacgtctca ttggaacacg accgccgtat ccaaattaac
     8581 atggggatat gcggataccg ctttaccaga tcgacgcctt caccagccgt ctcttctccg
     8641 gcaacccggc ggctgt
//
LOCUS       DATIFQ010000497        26094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1702042, whole genome shotgun sequence.
ACCESSION   DATIFQ010000497 DATIFQ010000000
VERSION     DATIFQ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 26094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26094
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702042"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            564..2816
                     /locus_tag="VLE22_24970"
     CDS             564..2816
                     /locus_tag="VLE22_24970"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HSB17725.1"
                     /translation="MNSLGLFRLVCVTTQLSVCVISMGCGTAPNPSRRGQGSGSEVAT
                     TINPAGPIVLKTAAAEFEILPSGYIRAYLLNGASRLSLDEPDGGSDFLISAGEEISNF
                     QLDFTRAKVMDAHGKVGTHGKRVEVTGRASAAAGAVIEKTLAVEVYDDFPKLAVTSLA
                     YRNAGDRAFAVDRVVAASHRLNAALGDAKAASYSLWSFQGSSYDWGKDDVLPLSAAFY
                     RPNVIGGPGPQGLGGGIPIVDFWTRSVGLAIGHIEPVPLVMSLPVRVEKDGRVRASIA
                     LEPRAVLKPGEAYSAPRSFVAVHSGDYYEPLEMWSRAIQRQSSWSIAKAIPQSYEANW
                     CGWGYESDFTTAQMLGTLPKLKQLGFRWATLDLRWFDNYGDWMPRPDTFPGDSIKNVV
                     DEYHKQGILVQLWYQPLSAEDGVGKHGLPKPMAMSRIIKEHPDWAILDKDGKRARLVS
                     AVSTGTAMCPALPAVQQYHRQLVQRFLGDWGYDGLKMDSVYSVPPCYNPKHHHQSPED
                     SIKAMGQVFQVIFETAREVKPESIVQICPCGTTPNLAWVFFQDQAVTADPVGSVQVRR
                     RIKMYKALLGPRAAVFGDHVELTDMKQVGTSTNAVTWVETGRDFASTVGAGGVIGTKF
                     TWPDLPPRPRYRDVVLTADKEAIWKKWIDIYNSKMLSRGNFLNLYTIGYDFPEGYAIE
                     KDGKMYYAFFLPEQSGVWKGEIELRGLKPGSYRVFDYANGKELGEVPAANPKLTAEFS
                     EHLLLEVSRQ"
     gene            2846..5047
                     /locus_tag="VLE22_24975"
     CDS             2846..5047
                     /locus_tag="VLE22_24975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17726.1"
                     /translation="MRKASLIALSFILAVSMLFGQAQEQRAYRGVTVSPSYSYHMSGR
                     TDWIKGYFVRIDDPQAIRNPGLVFLASGIDHAGEALLKVNGKDYDVPPLEGDFSGRAK
                     PTANEASGKSGFYAYERGGDDVVAKIVVPLKASDLRAGANQVEFYRSPDSDGFEIPAA
                     QVESVEQREPAVLGQTYHLLSRGRPATVRDFDAVIAVKPEDHRRAEEVPEWARRGMVN
                     FYRAGMPTDQLERMFQMFEEAHVNLISVGVPRQKDSEAYSRARALIERCHAKGLKAVG
                     FNSLGGVPLREVLLNPEVESWVARDEYGNKLWQVQGSSFAADISDKGYRRSVLDHAAL
                     QIDAGVDELYYDYALGGTGDVRTLFAEIRAIAKQKGKNISVFGNCKGNILVDEIADLV
                     KSEGTEEAGVWDGKWVHNIPQARFYYGVAEGGKRYESKYEGADPGVPNPGAHDVREGM
                     KCGWRKPIAEASAFQSEFAIAEAGEKLLSGWIHLNHPLAVQSWKEICTYFGFRHDNRD
                     LYTDVSTVSKIGVIAPPLIPSFEVSLRRESLYKALAEMNIMYEVLLLHRISADRLARY
                     KTVIITDIPWMEASQAAAIKTFKQAGGKIYAIGGAKKFRDMADVVSPADLFAKLSEPS
                     ARKTLAESLRRLEGEPLVMVEGAPYVAANLAKKNRQNRFFLHLVNYDRPLKDVRVKVN
                     LDGIAESINAKGLRLYSPDAVPKTVNVTRATGKSVEFVVPALEVYDVITFD"
     gene            5124..6395
                     /locus_tag="VLE22_24980"
     CDS             5124..6395
                     /locus_tag="VLE22_24980"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSB17727.1"
                     /translation="MEKSIATGRRAFLKWVGALPLLGYIATQGVLEKAFGAAKRNYSK
                     NIYTRIGVRPVINAPGPWTYISGTLELPEVKAAKQQAAEYFVDMWELQRAVGRRLAEL
                     SGAEAGMVTSGAAGAMAAATAACIAGTDPAKIWQLPDTTGLKNEVVMFGGRSLFDSAI
                     RLAGGRLVVANTYEELQAALNENTVMVYTTARGEGLEKALAITRKAGIPLLVDWAAGV
                     PPIENLSLLAKMGVDLYTFSGGKGLCGPQCTGLLLGRKDLIEAALANYNPWEGAVCRP
                     MKVGKEEIMGLLAAVEAWKKIDLAALNREWSRRVKRISKVVETVPGVTTETQTIYASE
                     SLTVKWDEAAFKLTVTECARKLRDGTPRIEVLTAHNPSAVPGAREGDPKNPRSPRADQ
                     LRIVSLTMQAGEELLVARRLREVLTEARKNA"
     gene            6573..8621
                     /locus_tag="VLE22_24985"
     CDS             6573..8621
                     /locus_tag="VLE22_24985"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HSB17728.1"
                     /translation="MVESRQLAILLMTMSLDAGLTGLLQAQARVEGKNIRIEFNSSLH
                     SRVIAKIGGKEIVVGPFSPSEFVTVSGTQVKDFRQTGQKTEPLADEFGRGRRLVITGE
                     AASLIKTVTVTIYDEFPRLAFYDVQYTNTGISDRQVTAWTNQHYSIAAGQPAGDPAFW
                     SYQSASYSKRPDWVLPLKPKFHQENFLGMNSSDYGGGTPVVDVWRRDVGIGVGHVELT
                     PKLVSLPVDQVDDHSAEVAIRYEHAETLKPGGTLKTFRTFVTVHRGDYFQTLTEYRRA
                     MLKRGIKFDPAPASAFEPIWCAWGFGKDFTPAQIDGALPIVKKLGFGWVTVDHGWQTT
                     EGDWCLNPKKFPHGDADMKALVRRIHANGQKAQLWWGPLIAGPESETLKAHPEQALLS
                     ADGAKQRITFWNGWYLCPADPQVVDYLRTIVVKALRDWDFDGLKLDGMHMNGAAPCYN
                     PAHKHTRPEDSVEAMPQVFKAIYDTARSVKPDVLIEWCPCGTSFNFYTLPYLNMSVAS
                     DPRGSFQIRSKGKTLKALHGDSTAYFGDHVELSTGRDDFASTIGVGGVVGTEFTWPPG
                     AGGRSRGELTPEKEQIWAKWIQIYRDKMLSRGEYLGDLYDIGFDRPETHAIRKAENMY
                     YSFYAPDFKGVVELRGLGSRSYTITDFVNGKTLGSLRGPAGRLEVQFQKYLLLEAKPG
                     "
     gene            complement(8618..10603)
                     /locus_tag="VLE22_24990"
     CDS             complement(8618..10603)
                     /locus_tag="VLE22_24990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17729.1"
                     /translation="MVVDIPGALANPGLVVLAEGLKAAGAGRVDLNGNPYEIPPSPGG
                     LLAKIVIPLRPDHLRAGLNRVVFSRSVGAESYQVVDARIEDVTERTGRDARVTYLPVS
                     AASTIANFDFVTNPDSTNKRKESELADWALRGKLRFYRAGVDLALLNRMFEMFKEAHI
                     NIVMLQMPTPTDTQTQEYKTYKDFFDRCHAAGIKIMYDGGNGAQPIRLNSISLESVLL
                     YPYMRDWISKDEYGIPRWRTPRRAFWPDLSNKDYRNEVLKVAEIALDAGADAFYYDWA
                     IGGTADLVRFFAELRDLTKRKNKNIPMYGNCKGNIVVEELCDFTKTEAIEEPGVWDGK
                     WVNNVAQARFYYAAGDGWKPYESKYEGADPGAPHPGAHDVKDGMKCGWKRPMAEAWAF
                     QSHWVIAEAGRTMFQGWVQKNNALAMTAWSDICRYNAFFDENEDLYTDVRTVSNIGLV
                     APPAIPSFELHLPRAPLYDALGELNFLYDVLLIPKLGEKDLAAYQSLVIPDISWLDDN
                     HLRLLEDYRRSGGQILTVGSSEAIRKISSATLPASTIQDIQKRSVRDEFKRELLKLSG
                     DPVVDVQNAEYVIVNLVRKRESPKVIAHFINYGKPLDNVTVRLNLRGTSKQIDTTHIR
                     LLSPDDVSKQLKDVSSRGPEVAFTIPSIDVYDVVVFN"
     gene            complement(10875..14567)
                     /locus_tag="VLE22_24995"
     CDS             complement(10875..14567)
                     /locus_tag="VLE22_24995"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB17730.1"
                     /translation="MLRRFAILSFLAVFIVVAVAWPQAGVSTVRGTARDPSQAVVPAA
                     TVKLTNIDTNVTRTTKTNDVGIFAFPSVIPGRYRITIEASGFQNFEGTLTVMVQQDAE
                     VDGVLQLGQTATQVEVADVTQMVRVDSPTLGHVLERQRIEQLPINGRSYQNLLATVPG
                     IDSTGIPQAYGMRTNTSVTLFDGTAVNESYEGWDFGRPPGLDSIAEFQMEVNNSSAKF
                     TRPATIILSSKSGTNVFHGSLFETNRNSGYGVARRRQDNFQKPPFLNRNEFGASAGGP
                     IYVPKLYDGRNRTFFFFAWEASRNLSSSTQQWSVPTEAMRNGDFRGLVDDSGRQYQLY
                     DPFTTDPVTWERQPLTYNGVANMIDPARISPTAKYLFSITPLPTLPQVNPLIDVNWVG
                     PFSGYTNGSNTSIRIDHRISDADLLYGRYSFNTYKRLTSYGGQPMLNGVAGTENYDRP
                     NHTVGVTWVHTLSPTMTNEVVGSASHENHFRGMGDRKTNYVRDILGLPNPFNEVDWPS
                     FEGFDLGEYTFGTDGLFTLITNFVSLQDNVTKIQGKHEFQFGVHTRFELLNNSRAAYP
                     NGPFSYATLGTSLYDPDSTPDDPLPFEYTGHGMANMYLGIMDYNVNFLRPAFDFRRRE
                     YAGYLQDNWKVTPRLTLNLGLRYEVRPPIYDARGTLIGFSLDKRAFVVGESVDKYLQQ
                     GISLPSIVNAIQGYGGKIIPYNEAGLPQHLMYTNWRELGPRLGFAYRALDGAKAFVVR
                     GGFRMSYYPQKMQDWAENQYSSPPGSATFSYSVTDPAFSPDGLPNYGLRSAPTYIAGV
                     NSTNDIIDVNNASTLTRGFSARVLPSNYTDGGVADWNFTLEKEVMASTVVRVAYVGNH
                     GYNQQQYVGYNDSTPSYVWYASRGEPLPTGEFAGVATRPYDQTVYGGINMFSSIGWSN
                     HTSFQFELERRYHNGLGFQVFYRTAKTLLTNRDTDGTQSGETIHSLNFYMPDAVPTDL
                     KERNRFLNYRMDPNTPHHRLQWNFIVELPFGRGKRFGTNMHSVLDKIMGGWQIAGLGS
                     VTSSWWSLPTTYYRTDQNIEVYGYKYPIQDCRSGRCYPGYLWWNGYIPSNLINSHDED
                     GNPNGIMGVPSDYKPAAQPLIPWGQTELPPNAPPGTDLESYWDTNTVWLKLNNGKTQR
                     VTYNTSLPPWRNQYSPGPRQWFQDASLFKFVRLSERLNLRFNVDFFNVFNNPNNPTSA
                     AANGVLATRNSGSAARTTQLTVRLSW"
     gene            14845..16866
                     /locus_tag="VLE22_25000"
     CDS             14845..16866
                     /locus_tag="VLE22_25000"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB17731.1"
                     /translation="MRHRGFGCLVALIALLACKSPDKSAPATEAGPVRRPRNVVLVTI
                     DTLRADHLHCYGYPSIETPVIDSLARRGALFENAVAQAPLTAPSHASIFTGQYPNVHG
                     VRNTGMFKLPPSATTLAKILQQQGWETAAFVGAAVLKKTFGLDQGFAIYDDEMPKAPR
                     ALQYGEYAERPAAEVVNRAVQWLNGQSGKPFFLWVHLFDPHAPYAPPAAYKLRYAGRP
                     YDGEIAYADHELGRLFDAADRKAPQDTTLTVLMADHGESLGDHGEFSHGVFLYDATLR
                     VPLVMVGPGIPPGRRVREQARTIDILPTILDLVGRQPPSAVQGISLAQAFLGNPLPAT
                     ISYAETLFPKMSMGWAELRAVRTSTWKYIQAPKPELYDLSRDGGETRNVILDRPAAAQ
                     QLRAQLNELSRTNRGPDKVQSSTVDPRTMEQLKSLGYASGFSPRQIELTGQGIDPKER
                     VEILKLEHLATGMEIEPPLARRIELLRKAVASDPANPLMYSHLGVAYAAAGRYQDALE
                     LYLSAMKHGVQSPKLYGHIAELYLRNGDNDRAIAYYEKAAQVDPADAQAQNNLAMAYL
                     EKGQATAAERVFRRVLSIDDQSADAYNGLGILAVRRQDLAEGRANFERAVELDPDLLE
                     ARVNLGLIYKKTGDRTRARACFKAFLAKASRAQYGPMISRVEQELALLR"
     gene            complement(16991..18376)
                     /locus_tag="VLE22_25005"
     CDS             complement(16991..18376)
                     /locus_tag="VLE22_25005"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB17732.1"
                     /translation="MRKRGLTRRGLFPASLAGAALAGKSASAVRPAEPATKYPVLSTV
                     DVLVAGGGPAGIGAALGAARAGANTLLVENHSFFGGVGAWALGMPINQVRPGGKPRSA
                     IHELLVHNLLRYGDQAVKIGDHQLWCNVEYLKVALLDALDAVGVRYLVHVRAADAIVE
                     HNRVAGVVIATKRGLMAVRASVVVDCTGDADVAYYAGAEVMIDPEQLMPMTLSLALTN
                     IDKAKTRFDDIEAAIRKARKKYPLVPAGFIELGTIAHGSSWYINHAGTADLGRLDATD
                     LVQRTQAECTSRRQALQMIQAVRESDNPSLRQIEWIGAGPQVGVRETRRVKGLYVITE
                     EDAMHGRRFEDAIAWRTGYLDFGGQKGAKFTKLKIHDVPYRCLVPEKLDGLLMAGRCM
                     SATHAGAAAGKSMSNCMATGHAAGLAAALAVGKRIPPRELSVHELQERLRADGVNLDV
                     RDRDQRDLRDA"
     gene            18501..19406
                     /locus_tag="VLE22_25010"
     CDS             18501..19406
                     /locus_tag="VLE22_25010"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17733.1"
                     /translation="MVRVLVILSVIVCSLRAQSLQQAISDVEAGRLENAEQALTRFLG
                     SHPDSEEALFYMGLLHFRAGRPAAARPYLDRAVKLSPTSAPAWKVLGLVTAAGGNLQE
                     ALTPFGKACQLAPSDEEACYYFARNLYALHRYDLACEPFAQALRAAPEGMRSKVDRAA
                     ALNLEALGRSAEAERHFREAVRLHRGDGRPNEDPHIDYGAFLFRQGRTAEALRTLEQA
                     TAALASSSRANTELGRVLLELGRPDAAAVQLERAIGLERGNWTARLLLGRAYLRLGRK
                     EEGNEQMRLGQEGWAREHYDSSIVK"
     gene            complement(19382..21073)
                     /locus_tag="VLE22_25015"
     CDS             complement(19382..21073)
                     /locus_tag="VLE22_25015"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB17734.1"
                     /translation="MQFQFIRDCFRVASGCAALAILAGWLPSAPAGPDPITFQNVASH
                     ARVDFILRNSATPEKHQIETMVSGVAVFDYNSDGWPDIYFINGARQPKLEKDTAAFYN
                     RLYRNNGDGTFTDITLQAGVKGEGFATGVATGDYDSDGFTDLFIVGVNRNILYRNRRD
                     GTFEDVTARAGLTHNSDRRKPWSIAAGWFDYDSDGWLDLFVVNYCVWIPGKEPPCSVN
                     GVRTYCSPKYYAGLPNSLYHNNGDGTFTDVSESSGIAAHIGKGMGVSFLDFDQDGRLD
                     VFVANDTYPNFLFRNEGGGRFREVALWAGLAFNEDGRALSSMGVDARDIDNDGREDVF
                     IAALANETFPLFRSLGKGLFADITYISGLGRQTLTATGWSNGVFDLNNDGLKDLFVAC
                     SSIDDNTEMFSPRKSRQANMVLVNLGGGRFLDVSTRAGPDFQQTGFHRGVAFGDFDRD
                     GRVDAVVSRIDERAELFRNTSTSGNHWLALRLRGHRSNRDGLGALVHVIGSSGRGQWN
                     RVTTSTGYGCASDRTVFFGLGKDAGAQSIEIEWPSGVRQRLEKVAGDRYLTIEES"
     gene            complement(21077..23065)
                     /locus_tag="VLE22_25020"
     CDS             complement(21077..23065)
                     /locus_tag="VLE22_25020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17735.1"
                     /translation="MRTVKLFALAFLLLAAGRSQDRQDWTHFVRIGGHGVSLSNVDQI
                     VRSATDTFVFGIEVDNDIPGRYESFLDPTEKLTALRAVAAKAHEAGNYAFVYIAGTEC
                     ITAKAASQAHSMAKDHPDWLQRKITGEPAVFGGGAAFWVREGDEDVWLTPFATDWMKT
                     YLERVRQIAATGIDGVYVDIPYWMTHFKGWGDSWVSFDDYTVAAFKRKTGLNAKTDLK
                     LGDFRDANFRKWIDFRIDALTDFMRQIDAAVKSVNPKCKTIAEIYPGIEDPAVRVGTD
                     VYDLYRVVDTIAHEYSTGGRAARRTPLTWFQDMVGMYSFRAFAGSKPTWMLTYSWENE
                     KEIVPGEAMKNLATAQLMAGTNTWDARGHVMSGSNDIETRKVIFQWIRDHEKVFFLPR
                     MPIRPIGVYFSPKSRNYFSKEFIDSYRGTMMLLMQSHLEFQVVTPRTLDAFHGSVLIL
                     PEARCLGKQELDVLRSRFNSGMTLIVTGETGKYDDTGAEQPANPIHKLLGIADPAQEG
                     AGTSRRKFLYYPRCPGAEYYAQLRKEFNELAASGEYQQAQFERLRTAWAAKLLGMSQI
                     KPAVEVETSPFISTQIAQVDGKTRVFLANFKGLKASQVAQQLPERNVRITFKTGRKGS
                     VYVLPFLGEVEKLAGEWKNGSLTCVIPEVNKGAVVWLD"
     gene            23168..24700
                     /locus_tag="VLE22_25025"
     CDS             23168..24700
                     /locus_tag="VLE22_25025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17736.1"
                     /translation="MKRRAFFRTAGLAIPIAVKPSAAYVVSHNWDKYDFGSGPPVKER
                     LYQGPFPQYPPEQIFPGGDVVMATTPSDEVVPNFGRGLITYIAADMGTAEILGEDKAK
                     AIEELVKLPLGQKLYIRPTWREVQPRRGRLEFPDYWKLTFDLAKQYNKQVGFRIQMRA
                     PDYPEEALPDFVLEKVPMVKLEGQWRRNSKREFQEPRYDHPAFQEAFQELNGLLAAEL
                     NGNPLVEFVDTFMYGFWGEGHTWPFRNNPFPNYQTAERTWAGMFEKQLEQWTKTPLAT
                     NTQPDFSLVGNSELVDRTVRSYNWLRTDTIFIENVQIEAIGNRPPWVAAVLEVGMSDG
                     SPNSLRISEGVPATDNMIQHVLDVGANYWSLWNFHRISAEGILNYYRQYPKKIDEINR
                     SIGYNVRPSFVWVYEGDNPGLIIGFANDGVAGVPGVLRVTVFGEDGKAYASGGLDAGY
                     PLPGKIRQAQFPLPKGTKWEGLQLKAELEVKGVRHPVRWACHQKTNPDGSLTLRRNLG
                     RG"
     gene            24746..25975
                     /locus_tag="VLE22_25030"
     CDS             24746..25975
                     /locus_tag="VLE22_25030"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HSB17737.1"
                     /translation="MNTPSLRIALCFVAGIAALAQPPYDLLLKGGHLIDPKNRVDAIR
                     DVAISSGKIAAVAEKIEPAKARRVVDVSGLYVTPGLVDIHVHAFAGTMAPEYTGALSV
                     RPDDFTFRNGVTTVVDAGSSGWRNFAEFKQQIIDNSRTRVLAMLNIVGRGMAGRTTVE
                     QDTTDMDPERTASEAARYPGLIVGIKIAHYAGPEWTAVERAVEAGRRADLPVMVDFAE
                     FRPERPFQDLVLRKLRPGDIYTHLYLGAVPMLDPQGRVLPYLLEARKRGVIFDVGHGA
                     GSFLFRQAVPAVRQGLVPDSISTDLHVSSMNAGMKDMLNVMSKFLNMGMSLEDVILRS
                     TWNPAREIRHPELGHVSVGSSADIAVLRIVDGNFGFVDSSHRRLQGTKRLVAELTLRD
                     GRVVWDLNGLASDDWAK"
BASE COUNT         5160 a         7753 c         8047 g         5134 t
ORIGIN      
        1 gtcttgcagt ttcatcatcc gctccatctc ggcggccgag tactgccggg tctccctcac
       61 ccagccagcc tctcagaccg caatcctcaa agcggacaga tcatctgtga attccaccgg
      121 acagatcaca tgttaacgac agggcgtcca ggcggggatt gcgttccggc tcccgatccg
      181 gtacgatgcg tctcttcggt aacgttcacg aaggatgccg ccctatgcct tcttggtttc
      241 aaatgatccg gagcgatcgg cggatcattc tgtccgtcag cgtgaccctc gtcttcctgg
      301 cgaagccgag tccgatcctc ggccagatca agtacggcga aacaccgggg gtggtccaaa
      361 gacgcacgct gctgctccac gacttcaagc cgagatcgat gcttcagacc gccgcgcatg
      421 cagtccagcc ggcaagacac cctgtcatcg atgggacggt ggatgatata cgggctggag
      481 ttgcccgacc cggtcctgga gaaggtctat caccggaacg cagaaagaat cctcagccag
      541 ttccagagtg cgggagttag ccgatgaatt ctctaggact gtttcgtctg gtctgtgtta
      601 cgacgcagct ttcggtttgt gtgatctcga tggggtgcgg cacggctccg aacccgagcc
      661 ggagggggca aggatcggga tcggaggtgg ccacaaccat caaccccgcg ggtcccattg
      721 tcctcaagac ggcggcggcg gagttcgaaa tcctaccgag tggctacata cgggcgtacc
      781 tactcaacgg cgctagccgg ttgtccttag acgagcctga cggtggcagt gactttctga
      841 tcagcgctgg cgaggaaata tcgaattttc agcttgactt tactcgtgcc aaagtcatgg
      901 acgcccacgg caaggtagga acgcacggca agcgcgttga ggtcacaggg cgcgcttccg
      961 cggccgccgg cgccgtcatc gagaaaacgc tagcggtcga agtctatgat gatttcccca
     1021 aacttgcggt gaccagcctg gcgtacagga acgccggcga tcgcgcattc gcagtagatc
     1081 gagtggtggc tgccagccat cgtctcaatg ctgcactcgg cgatgcaaag gccgcgtcct
     1141 acagtctctg gtccttccaa ggatcgagct atgactgggg caaggatgat gtgctgccgc
     1201 tctccgccgc cttctaccgg ccgaacgtga ttggcggccc cgggccgcaa ggcttgggcg
     1261 gtgggatccc gatcgttgat ttctggaccc gcagcgtcgg gctcgccatc ggccatatcg
     1321 agccggtgcc cttggtgatg tccctgccgg tccgggtaga gaaagacggc cgcgttcggg
     1381 cgtctatcgc gctcgagccg cgggctgttc tgaaaccagg cgaggcatac tcggccccgc
     1441 gcagttttgt cgccgtgcat tcgggggact attacgaacc cctggagatg tggtcgcggg
     1501 ccatccagcg gcagagtagt tggtcgatcg ccaaagctat tccgcagtcc tatgaggcaa
     1561 actggtgcgg ctggggctac gagtctgatt tcaccaccgc gcagatgctc ggcacgcttc
     1621 ccaagctcaa gcagttaggc ttcaggtggg cgacgctgga cttgcggtgg ttcgacaatt
     1681 acggcgactg gatgccgcgc cccgacacgt tccccggtga ctcgattaag aacgtcgttg
     1741 atgagtacca caaacaggga atcctggtgc agctctggta ccagccgctc tcggctgagg
     1801 acggcgtggg aaaacatggc ctgcccaaac cgatggcgat gtcgagaatc atcaaagagc
     1861 acccggattg ggcgattctc gataaagatg gaaagcgtgc acgcctggtc agcgcggtta
     1921 gcacgggtac ggcgatgtgc ccggcgctgc cggcagtgca gcagtaccat aggcaacttg
     1981 tccagcggtt cctgggtgac tggggatacg acgggttgaa gatggattcc gtctattccg
     2041 tcccgccctg ctacaacccg aagcaccatc accaatcgcc ggaagactcc atcaaggcga
     2101 tgggacaggt gtttcaggtc atctttgaga ctgcgcgtga ggtaaagccg gaaagcatcg
     2161 tccagatttg cccttgcgga accacgccca acttagcctg ggtatttttc caggatcagg
     2221 cagttactgc cgatcccgtg ggcagcgtcc aagttcggcg ccggatcaaa atgtacaagg
     2281 cgctgctcgg cccgcgagcg gccgtgttcg gtgatcacgt cgaactcacc gacatgaagc
     2341 aagtgggcac gtcgaccaac gcagtcacgt gggtggagac gggcagggat tttgcctcga
     2401 ccgtaggcgc gggcggcgtc atcggcacca agttcacttg gcccgatctt ccaccgcgcc
     2461 ctcgttaccg cgacgtcgtg ctcacggcgg acaaggaagc gatctggaag aagtggatcg
     2521 atatctacaa ttcaaagatg ctctcgcggg gcaacttcct gaacctgtac acgatcgggt
     2581 acgactttcc ggaaggctac gccatcgaga aggacggcaa gatgtactat gcgttttttc
     2641 tgccggaaca gtcgggcgtg tggaagggtg agattgagct acgcgggctc aagcccggca
     2701 gctaccgtgt gttcgactac gcaaatggaa aggagctggg cgaagttccc gcggcgaacc
     2761 ccaagctgac ggcggaattc tccgagcacc tgctgctaga agtgagcagg cagtgagggc
     2821 gccgcggagc ggccaaggtg atcacatgag gaaagcttct cttatcgcat tgagttttat
     2881 cctggctgtt tcgatgttgt tcgggcaagc ccaggagcag cgtgcttatc ggggtgtgac
     2941 cgtcagtccg tcttattcgt atcacatgtc cggacgaact gactggatca agggttattt
     3001 cgtccggatc gacgatccgc aagcgatccg gaatcccggt ctcgtgttcc tggcgagcgg
     3061 tatcgaccat gccggcgagg ccctcttgaa agtcaacggc aaagactatg acgtgcctcc
     3121 gttggaaggc gacttcagtg ggcgggccaa gcccaccgcg aatgaggcct cggggaaatc
     3181 cggcttctat gcctacgagc gaggtggcga cgacgtggtt gcgaagatcg tcgttccgtt
     3241 gaaggcgagt gatctgagag ctggggccaa ccaggtggag ttttacagaa gccccgacag
     3301 tgatggattc gaaattcccg ccgcgcaggt ggaatcggtc gagcagagag agcctgcggt
     3361 tttggggcag acctaccacc tgttgtcccg cggacggccg gcgacagtcc gtgacttcga
     3421 tgcggtcatt gctgtcaaac cggaggacca tcgccgcgcc gaggaggtgc ccgagtgggc
     3481 caggcggggc atggtgaatt tttatcgtgc cgggatgccc acggatcagc tcgagcgaat
     3541 gttccagatg ttcgaggagg ctcacgtcaa cctgatctcg gtcggggttc cccgtcagaa
     3601 agacagtgaa gcctacagcc gcgcgagagc gctgatcgag cgatgtcacg caaaaggcct
     3661 caaggcggtg ggtttcaatt ccctgggcgg agttcctctc agggaagttc tcctaaaccc
     3721 ggaagtggag agttgggtcg cgcgcgacga gtacggaaac aaactctggc aggtccaggg
     3781 gtcctcgttt gccgccgata tctccgacaa gggttaccgc cggtctgttc tcgatcacgc
     3841 agcgctacaa atagacgctg gcgtcgacga actctattac gattacgccc tcggaggcac
     3901 cggcgatgtt cgcacgctgt tcgccgaaat ccgcgccatc gccaaacaga agggcaagaa
     3961 catctcggtg ttcggcaact gcaagggcaa catcctggtg gacgagattg ccgacctggt
     4021 gaagtccgaa ggcaccgaag aggcgggcgt ttgggacgga aaatgggtgc acaacatccc
     4081 gcaggcgcgg ttctactacg gcgtcgccga gggcggcaaa cgctatgaga gcaaatacga
     4141 aggtgccgac ccgggcgtgc ccaatcccgg cgcgcacgac gtacgcgaag gaatgaaatg
     4201 tggctggagg aaaccgatcg ccgaggcgtc ggccttccag tcggagttcg ccattgccga
     4261 ggcgggcgag aaactcctga gtggctggat ccatctgaac catccgctcg cggtgcaatc
     4321 gtggaaagaa atctgcacat acttcggatt ccgccacgac aatcgcgact tatatacgga
     4381 cgtatccacg gtcagcaaga tcggggtgat cgcgccgccg ctgatcccca gcttcgaggt
     4441 ctcgctccgg cgcgaaagcc tctacaaagc cctggccgag atgaacatca tgtatgaggt
     4501 gttgctgctg caccggatct cggccgacag gctcgcccgt tataagaccg tcatcatcac
     4561 cgacatccct tggatggagg catcccaggc agcggccatc aagacgttca agcaggcggg
     4621 cggcaagatc tacgcgatcg gcggcgcgaa gaagttccgc gacatggctg atgtcgtatc
     4681 gcctgcagac ctgttcgcga agctgtcgga gccttcggcc cggaagacgc tggcggaatc
     4741 tctgcgccgt ctggaaggcg agcccttggt gatggtggaa ggagccccgt atgtcgcagc
     4801 caaccttgcc aagaagaacc gccagaatcg gttcttcctt cacctggtga actatgaccg
     4861 gccgctcaag gacgtgcggg tgaaagtgaa tctcgacggc atcgccgagt cgatcaatgc
     4921 caaaggtctg cgcctgtact ctcctgacgc ggttcccaag acggttaatg tcaccagggc
     4981 caccggcaag tcggtcgagt ttgtcgtgcc ggctctggag gtgtacgacg tgatcacctt
     5041 cgactagctc agggtggccg gacatcgtcc gccgccgcat cgaggaggga tgaaagcgtg
     5101 aagtggagtc aagaggagga cctatggaga agtccatcgc aacgggtcgg cgcgcgtttc
     5161 tgaaatgggt cggcgcgctt ccgctgcttg ggtatatagc cacccagggc gttctggaaa
     5221 aggccttcgg cgccgcgaaa cggaactaca gcaagaacat ttacacgcgc atcggcgtca
     5281 ggccggtaat caacgccccg gggccctgga cttacatcag cggcacgctc gaactgcccg
     5341 aggtcaaggc cgccaagcag caggcggccg agtacttcgt ggacatgtgg gaactgcaac
     5401 gcgccgtggg ccggcggctg gcggagcttt ccggcgccga agcgggaatg gtcacctccg
     5461 gcgcagcggg cgccatggcc gcagccaccg cggcctgcat agctgggact gatccggcca
     5521 agatctggca gcttcccgac accaccgggc taaagaacga agtcgtgatg ttcggtgggc
     5581 gcagtctgtt cgacagtgcg atccggctgg ctgggggcag gctcgtggtc gcaaatacct
     5641 atgaggagct gcaggcggcc ctgaacgaaa acacggtcat ggtttacacg accgctcgcg
     5701 gggaggggct ggagaaggct ttggccatca ccaggaaggc gggcataccc ttactggtgg
     5761 actgggcggc cggcgtacct ccgatcgaga acctatcact gctggccaag atgggcgtcg
     5821 atctatacac attcagcggg ggcaagggtt tgtgcggtcc ccagtgtacg ggccttctgc
     5881 tgggccgaaa ggacctgatc gaggccgcgc tcgccaacta caatccctgg gaaggggcag
     5941 tatgccggcc catgaaagtg ggcaaagaag agatcatggg cctgctcgca gcggtggaag
     6001 catggaagaa gatcgacctg gccgctctga atcgggaatg gagcaggcga gtgaagcgca
     6061 tctcgaaagt ggtggagaca gtgccgggcg tgaccacgga gacgcaaacg atctatgcgt
     6121 ctgaaagcct cacggtgaaa tgggacgaag cggccttcaa actcaccgta accgaatgtg
     6181 cccgcaagct gcgggacggc acgccgcgca ttgaagtcct gacggcgcac aatcccagcg
     6241 cggttcccgg cgcccgcgag ggtgacccca agaacccgcg ctcaccgcgc gccgaccaac
     6301 tgcgaatcgt ttcactgacc atgcaggccg gtgaggaact gctggtggct cggcgcctgc
     6361 gcgaggtctt gaccgaagcg cggaaaaacg cttgaacgtg tcgggagacc agtgaatgag
     6421 gaactatgtc ttgctcgcat gcctgctagg gttagcgtca ggggcgaagg cggctcagcg
     6481 atctctgatc caagtgcgaa caagcgccgc cgcccgattt tgctcaagag cagtgcagaa
     6541 gatgtagatt gcccggaccc aggggaatcg ttatggtcga aagcagacag ttggctatcc
     6601 ttctcatgac tatgagcctc gatgcaggcc tgacgggtct cctgcaggcc caagctcgcg
     6661 tggaagggaa gaatatccgc atcgagttca actcatcgct gcacagccgc gtcatcgcca
     6721 aaataggagg caaggagatc gtcgtcggcc cgttctcgcc gtccgaattc gtgaccgtga
     6781 gcggaaccca agtgaaggat ttccgtcaga cggggcaaaa gacggagccg ctggcggacg
     6841 agtttggccg cggccggcgc cttgtgatca cgggtgaggc tgcgtcgctc atcaagaccg
     6901 tgacggtcac catctatgac gaattcccgc gcctggcgtt ctacgacgtt caatacacaa
     6961 ataccgggat ctcggacagg caagtgacag cctggacgaa tcagcactac tcgatcgctg
     7021 ccggacaacc cgccggcgac ccggccttct ggtcctatca gagcgcgtct tactcgaagc
     7081 ggccggactg ggtactcccg ctcaagccaa agttccacca ggaaaacttt cttgggatga
     7141 actcttctga ttacggcggc ggcacgcccg ttgtcgatgt ctggcgcagg gatgtgggga
     7201 tcggcgtagg ccacgtcgag ttgactccca agctggtatc gctaccggtg gaccaggtag
     7261 atgatcactc agcggaggtc gccatcaggt atgagcacgc ggagactctc aaaccaggcg
     7321 gtaccctgaa gacgtttcgt acgtttgtca ccgtgcaccg gggcgattac tttcaaacgt
     7381 tgaccgagta tcgcagggcc atgctcaagc ggggcatcaa gtttgacccg gcgccggcca
     7441 gcgccttcga gccgatttgg tgtgcgtggg gctttggcaa ggattttact cctgcgcaga
     7501 tcgacggcgc gctgcccatt gttaagaagc tgggctttgg gtgggttacg gtagaccacg
     7561 gctggcagac cacggaaggg gattggtgcc tgaacccgaa gaagtttccg cacggcgacg
     7621 cggatatgaa agccctcgtc cggcgaatcc acgcgaatgg acagaaggcc caactctggt
     7681 ggggacctct catcgcagga cccgagagcg aaacgctcaa ggcccatccg gagcaggcgc
     7741 tgctgagtgc ggatggtgcg aaacagcgga tcacgttctg gaacggatgg tatttgtgcc
     7801 cggccgatcc gcaggtggtg gactatttgc ggacgatcgt cgtgaaagcg ttgagggatt
     7861 gggacttcga tgggctgaaa ctggacggca tgcacatgaa cggcgcggct ccatgttaca
     7921 acccggcaca caagcatact cggcccgagg actcggtcga ggcgatgccc caggttttca
     7981 aggcgatcta cgacacggcg cgaagcgtta agccggatgt actcatagag tggtgcccgt
     8041 gcggcacgtc gttcaacttt tacacgctac cttatctgaa tatgagcgta gcgtcggacc
     8101 cgcgagggtc gttccaaatc cgatccaaag ggaagacgct caaagcccta cacggggact
     8161 cgacggcata cttcggcgat cacgtagagc tcagcaccgg ccgggacgat ttcgcctcca
     8221 cgatcggcgt cggtggtgtg gtgggaacgg agttcacctg gccgccaggc gctggcgggc
     8281 gcagtcgtgg cgagctgaca ccggagaagg agcagatctg ggcgaagtgg atccagatct
     8341 atcgggacaa gatgctgtcg cgaggcgaat acctgggcga cctgtacgat attggctttg
     8401 accggccgga aacccatgcc atccgcaagg ctgagaatat gtactactcc ttttatgcgc
     8461 ccgactttaa gggtgtagtc gaactccgag gcctaggcag ccgcagctac acgatcacgg
     8521 actttgtgaa cgggaaaact ctgggctcgc tccgtggtcc ggccgggagg ttggaagtgc
     8581 aattccagaa atacctgctg ctcgaagcca agccgggcta attgaaaacg acgacgtcgt
     8641 atacgtcgat gctgggtatc gtgaacgcca cctccgggcc ccggctcgag acgtctttga
     8701 gctgcttgga aacatcgtcc ggggagagca gccggatatg tgtggtatcg atctgcttcg
     8761 aggttccccg gaggttcaat cgcacggtca cattgtccaa aggcttaccg taattaatga
     8821 agtgcgcgat caccttgggt gattccctct tcctgactag gttcacaatg acatactccg
     8881 cgttctgtac gtcaaccacg ggatcgcccg acagtttcag caactcgcgt ttgaattcgt
     8941 ctcttacgga ccgcttctga atgtcctgaa tggtcgaggc cggcagggtt gccgaggaga
     9001 tcttccgtat ggcctcggag cttccaacgg tcaggatctg acctccggag cgcctatagt
     9061 cttcgagcag ccgaaggtga ttgtcatcca gccaggagat atccgggatc accagtgatt
     9121 ggtatgctgc cagatccttt tcgcccagct tggggatgag caggacatcg tacaagaaat
     9181 tcagttcgcc cagcgcgtcg tagagggggg cccgagggag gtgcagttcg aagctaggta
     9241 tcgccggagg cgccacgaga ccgatgttgc tcaccgtcct cacatccgtg tagagatcct
     9301 cgttctcatc gaagaatgcg ttgtaacggc atatatcgct ccaagcagtc atcgcaaggg
     9361 cgttgttttt ctgcacccag ccttggaaca tagtccggcc agcctcggct atcacccaat
     9421 gagactggaa tgcccatgcc tccgccatcg gcctcttcca accgcatttc atgccatctt
     9481 tcacatcgtg cgctccggga tgcggggccc ctggatcggc cccctcgtac ttgctttcgt
     9541 aaggcttcca cccatcgccc gctgcatagt aaaaacgcgc ctgggcaaca ttattcaccc
     9601 acttgccgtc ccacacgcct ggctcctcga tcgcctcagt cttggtgaaa tcgcatagct
     9661 cctcgacgac gatattgccc ttgcagttgc cgtacatcgg gatgtttttg ttctttcgct
     9721 tcgtcaggtc acgaagctcg gcgaagaacc gcaccaggtc ggcggtcccg ccgatggccc
     9781 agtcgtaata gaacgcatcg gcgccggcat cgagagcgat ctcggcgacc tttaggactt
     9841 cgttcctgta atccttgttg ctcagatccg gccaaaaagc ccgtcggggc gtgcgccagc
     9901 gaggaattcc gtattcgtcc ttgctgatcc agtctctcat gtaagggtac aaaagaacgc
     9961 tttcgaggct gattgaattc agccggatgg gctgggcgcc gttgccgcca tcgtacatga
    10021 tcttgattcc tgcggcgtga catctgtcaa agaagtcttt gtaagtcttg tactcctgag
    10081 tttgtgtgtc agtcggagtc ggcatttgca gcatcactat gttgatgtga gcttccttga
    10141 acatctcgaa catccggttg agaagcgcaa ggtccactcc ggcgcgataa aagcgcaatt
    10201 tgccccgcag cgcccaatcc gcaagctcgc tctccttcct tttgttcgtg ctgtcgggat
    10261 tggtgacgaa gtcgaagttg gcgatcgtgg aagccgcaga gacgggaaga tacgtcaccc
    10321 gcgcgtcacg cccggtccgc tcggttacat cttcgatgcg ggcatccacg acctggtagg
    10381 actcggcgcc aacgcttctg gaaaatacca cccgattaag ccccgcgcgg aggtgatccg
    10441 gcctgagagg gatgacgatc ttggcgagca agccgcccgg cgatggtggg atctcgtacg
    10501 ggttgccgtt caggtcaacc ctgccggcgc ccgctgcctt gaggccctcg gccagcacca
    10561 cgagtcccgg gttagcgagc gcaccgggaa tgtcgacgac cactacatac gctctaactt
    10621 caccagaccg cgagtcccgg ccggtagctc gtggcgagct gcggtcggat ccaccggaaa
    10681 cgctgataca ctgatacgtc ttggccgggg cagaatacat tgtggaaccc ggaaagacga
    10741 gcaacgacgc aacaagcgct acaccataac aattcctcgg aatcatttca gcctctcgaa
    10801 ttatcgctgg aagacttgcc ggggtctgtc cgaatgagga gggagcggcc ggcacggccg
    10861 gccgctccaa gctgttacca cgagagcctt acggtcaact gagtcgtccg tgccgcgcta
    10921 cccgagttgc gggtggcgag cacgccgttg gccgcggcgc tggtggggtt attcgggttg
    10981 ttaaacacgt tgaagaagtc gacgttgaag cgtaggttca gcctctcgct gagcctcacg
    11041 aatttgaaca gcgaggcgtc ctggaaccac tgccggggac ccggtgagta ctggttgcgc
    11101 cacggcggca ggctggtgtt gtaagtgacg cgctgcgtct ttccattgtt gagcttgagc
    11161 cacacggtgt tggtgtccca gtacgattcc aggtcagttc ccggcggggc gttcggcggc
    11221 aattccgtct gtccccaggg tatcagcggt tgggccgccg gcttgtagtc tgatggcact
    11281 cccatgatcc cgttgggatt gccatcctca tcgtggctgt tgatcaagtt cgacgggata
    11341 tagccgttcc accataagta gcccggatag caccgcccgc tgcgacagtc ctgaatcggg
    11401 tacttgtagc cgtagacctc gatgttctga tcggtccgat agtaagtggt cggcagcgac
    11461 caccaactac tagtcacact gccgagacca gcgatctgcc agccgcccat gattttgtcc
    11521 agaacgctat gcatgttggt gccgaagcgt ttcccccggc cgaacggcag ctcgacaatg
    11581 aagttccact ggaggcggtg atgcggggtg ttgggatcca tccggtagtt caggaaccgg
    11641 ttccgttcct tcaagtccgt ggggaccgcg tccggcatgt agaagttcag cgagtggatc
    11701 gtctccccgc tctgtgtgcc gtcggtgtca cggttggtca ggagcgtctt ggctgtccga
    11761 tagaacacct ggaagcccag cccgttatgg tagcgccgct cgagttcgaa ctgaaagctg
    11821 gtgtggttgg accagccgat cgagctgaac atgttgatgc cgccatagac ggtttgatcg
    11881 taaggccggg tcgcgacccc agcgaactcg ccagtcggca acggctcgcc tcggctggca
    11941 taccaaacat aggacggggt ggagtcgtta taacccacgt actgttgctg gttgtaccca
    12001 tggttgccga cgtaggccac tcgcactacc gtgctcgcca tcacttcttt ctcgagggtg
    12061 aagttccagt ccgccacccc gccgtcggtg tagttggacg gcaacacccg cgccgagaag
    12121 ccccgggtga gcgtgctggc attgttgacg tcgatgatgt cgttcgtgct gttcaccccg
    12181 gctatgtacg tcggcgcgct tcgtagcccg tagttgggca ggccgtcggg cgaaaacgcc
    12241 ggatcagtca cgctgtaaga gaacgttgcc gacccgggcg gcgaagagta ttggttctcc
    12301 gcccagtcct gcattttctg cgggtagtag gacatccgga aaccgccgcg caccacgaac
    12361 gccttggctc catccagcgc tcgataggca aagccgagcc gcgggccgag ttccctccag
    12421 ttcgtgtaca tcaggtgctg cggcaggccg gcctcgttat aggggatgat cttgccgcca
    12481 tagccttgaa tggcgttcac aatcgaaggc agcgatattc cctgctggag atacttgtcg
    12541 accgactcac ccaccacgaa cgcccgctta tcgaggctga aaccgatcag cgtgcccctc
    12601 gcatcgtaga tcggcggccg gacctcgtag cgcaagccca agttcaacgt aagccgcggc
    12661 gtgaccttcc agttgtcttg aaggtacccg gcgtattccc gccggcggaa gtcgaaggcc
    12721 ggccgcagga agttcacgtt gtagtccatg attccgagat acatgttggc cattccgtgg
    12781 cccgtgtact caaatggaag cgggtcatcg ggggtcgaat cggggtcgta tagcgaagtc
    12841 cctagcgtgg cgtagctgaa cgggccgttc gggtacgctg cccgggagtt gttcagtagt
    12901 tcgaaccgag tatggacgcc gaactggaat tcgtgttttc cttggatctt ggtgacattg
    12961 tcctggagcg aaacgaagtt ggtgattaga gtgaacaggc cgtcggtgcc gaacgtgtac
    13021 tcgccgaggt caaagccctc gaagctcggc cagtcgactt cgttgaacgg gttaggcagc
    13081 cccaggatat ctctgacgta gttcgtcttg cgatcgccca ttccccgaaa gtggttttcg
    13141 tgggacgcgc tgcccaccac ttcattggtc atcgtcgggg acaaggtgtg tacccacgtg
    13201 acccccacgg tgtggttcgg gcggtcgtag ttttcggtgc cggcgactcc gtttagcatc
    13261 ggctggccgc cgtagctggt gagccgcttg taagtgttga acgagtaccg gccgtacagc
    13321 aggtccgcat ccgagatccg gtggtcgatg cgaatactgg tgttgctgcc gttggtgtag
    13381 ccagagaacg gacccaccca gttgacgtcg atcagcgggt tcacctgggg caaggttggc
    13441 agaggggtaa tgctgaacag gtacttggcc gtggggctga tccgggccgg gtcaatcatg
    13501 ttcgccacgc cgttgtaggt gagcggctgc cgctcccagg tgaccggatc ggtggtgaac
    13561 ggatcgtaaa gctggtactg ccggccggaa tcatccacca gcccgcggaa gtccccgttt
    13621 cgcatcgctt cggtcggtac tgaccattgc tgggtcgagg aagagaggtt gcgtgaggcc
    13681 tcccaggcga agaagaagaa cgtccgattt cgcccgtcgt acagcttggg aacataaatc
    13741 ggccctccgg ccgaggcgcc gaattcgttg cggttcaaaa acggcggttt ctggaagtta
    13801 tcctgccgcc ggcgggccac accgtagccg ctgttgcggt tcgtttcgaa cagcgaacca
    13861 tggaaaacgt tcgtaccgct cttgctcgat aggatgatcg tggccggccg cgtgaatttc
    13921 gccgaggagt tattgacttc catctggaac tccgcaatag aatccaatcc cggcgggcgc
    13981 ccgaagtccc atccctcgta gctctcgttc accgccgtgc cgtcgaacag cgtcacgctg
    14041 gtgttagtcc gcatcccata cgcctggggg atgccggtgg aatcgatacc cggaacggtg
    14101 gctagcaggt tttgatagct gcgcccattg atcggcagct gctcgatgcg ctgccgttcg
    14161 agcacgtggc ccagcgtcgg agagtcgacc cttaccatct gggtgacgtc tgcaacctcc
    14221 acctgagtag cagtctggcc gagctgcaga actccgtcca cctcggcatc ctgctgcacc
    14281 atcaccgtca gcgtgccctc gaagttctgg aagcctgagg cctcgatggt tatccggtag
    14341 cgacctggaa tcacgctggg gaatgcaaaa atgccgacat cattggtctt ggtggtgcgt
    14401 gtaacattgg tgtcgatatt ggtcagcttg accgtggctg cgggaacaac ggcctgcgat
    14461 ggatcgcgag ccgtaccccg gaccgtgctg acaccagcct gcggccaggc cacggccacc
    14521 acgatgaaaa ctgccagaaa ggaaagaatg gcgaaacgtc tcagcatggg ccctccttgg
    14581 tccccccctg tgtcgatatc ccgacacata cgagaccgac cccgagcaac gtgaccagac
    14641 agccctgtgc ccccacgcgg aagcggtagg gttaacgtcc tgaagattag tgcaatctgg
    14701 cgagtttgtc aagggtcatt ttttcgaaag cccttgggag gatttgccag ctcacggcgt
    14761 cgcgcgtgga cgtacacgcg gcaatccgcc gccaccgccc cgaagctcat tccggcagat
    14821 acgatggcat atactgggcg gtgaatgcga caccgcgggt tcggctgtct tgtagcgctg
    14881 attgcgcttc tggcctgcaa gtccccggac aagagcgcgc cggcaaccga agccgggcct
    14941 gtccggcgcc cgcgtaacgt cgtcctggtc accatcgata cgctgcgtgc cgaccatttg
    15001 cactgttacg gatacccgag tatcgaaacg cccgtcatcg acagtctcgc gcgcagaggc
    15061 gctctgtttg aaaacgccgt tgcacaggcg ccgctcacgg ccccctccca cgccagcatc
    15121 ttcaccgggc agtatccgaa cgtgcacgga gtgcgcaaca cggggatgtt caagctgccg
    15181 ccttcagcga ctactctggc gaagatcttg cagcagcagg ggtgggaaac ggccgcgttc
    15241 gtcggagcgg cggtcctaaa gaagaccttc ggcctcgacc aaggctttgc catctacgac
    15301 gacgagatgc cgaaggcgcc cagggcgctc cagtacggcg aatacgccga gcgtccggcg
    15361 gcagaggttg tcaaccgggc cgtgcagtgg ctcaacgggc agtccgggaa gccgtttttc
    15421 ctgtgggttc acctcttcga cccgcacgcg ccgtacgcgc ccccggctgc ctacaagctg
    15481 aggtacgcgg gaaggccata tgacggggag atcgcctacg ccgaccacga gttgggccgg
    15541 ctgtttgacg ccgccgaccg aaaggctccg caggacacga cgttgaccgt cctcatggcg
    15601 gatcacggag aaagcctcgg cgaccacggc gagtttagcc acggtgtttt tctctacgat
    15661 gcaacgctgc gggtcccgtt ggtgatggtt ggccccggga ttccccctgg ccgacgtgtg
    15721 agagaacaag cccggaccat cgacatcctt ccgactatcc tggacctggt gggccgccag
    15781 ccaccttctg ccgtacaagg tatcagcctg gctcaggcgt ttctcggtaa cccgctccct
    15841 gcaaccatct cctacgccga gactcttttc ccgaagatga gtatggggtg ggcggaactg
    15901 cgggctgtgc gcacgagtac ctggaagtac atccaggcgc cgaagccgga gctctacgac
    15961 ttgtcgcggg acggcgggga gaccaggaac gtcattctgg accggcccgc agcggcacag
    16021 cagctcaggg cgcaactgaa tgagttgtcg cgaacgaacc ggggtccgga caaagtccag
    16081 agcagtaccg tcgacccgcg cacgatggag cagttgaagt cgctcggcta tgcctccggt
    16141 ttttcgcctc ggcagatcga gcttacgggg cagggcatcg acccgaagga acgggtcgag
    16201 atactgaaac tggagcacct cgcgactggc atggaaatcg agccgccgct cgcccgcagg
    16261 attgagctcc tccgcaaagc ggttgcttca gatcctgcca atccgttgat gtattctcat
    16321 ctcggcgttg cctacgcggc ggcagggcgc taccaggatg cccttgagct ttacctgagt
    16381 gcgatgaagc acggcgtgca gagcccgaag ctatacgggc acatcgccga actgtacctg
    16441 agaaacggcg acaatgaccg ggcaatcgca tactacgaga aggcggcgca ggtcgatcca
    16501 gccgatgcgc aggcgcagaa caacctagcg atggcctatc tcgaaaaggg ccaggcgacc
    16561 gcggcggagc gcgtgttccg ccgggttctc agtattgacg atcaatccgc cgatgcatac
    16621 aacggcctcg gcatccttgc cgttcggagg caggacctgg cggaagggcg cgccaacttc
    16681 gagcgcgcgg tggaactcga cccagatctt ctggaagcgc gggtcaacct cggtctcatc
    16741 tataagaaga cgggtgacag aacccgggcc agagcgtgtt tcaaagcgtt tctggccaag
    16801 gcctcacgcg cgcaatacgg ccccatgatc tctcgggtag agcaggaact ggcgcttcta
    16861 cgttaggcca cagttcgcgt tggcggtcta ttgatttaca gccgggccgt agtgtctatc
    16921 gccctctatg gctccgggct tctgcttcca ttaatcccga agggataccg agggcggaac
    16981 ccttgcctga ctaggcatct ctcaggtccc gttgatcccg gtcgcgcacg tcgagattca
    17041 cgccgtcggc gcgcaacctc tcctggagtt cgtgcacgct cagttcgcgg ggcggaattc
    17101 gcttccccac tgccagggcc gccgccagac cagccgcatg gccggtggcc atgcaattgc
    17161 tcatgctctt gccggcagcg gccccggcgt gggtagcgga catgcaacgt ccagccatga
    17221 gcaggccatc gagtttctcg gggaccaggc accggtaagg gacgtcgtgg atcttcaact
    17281 tggtgaactt agctcccttt tgtcccccaa aatccaggta tccggtccgc caggcgattg
    17341 cgtcttcaaa ccgccggccg tgcattgcgt cttcttcggt gatgacgtac aggcccttca
    17401 cacggcgggt ttcgcgcacg ccgacctgcg gcccggcgcc gatccactcg atttgacgga
    17461 gactcggatt gtccgactcc cgcaccgctt gaatcatctg aagcgcctgg cggcggctgg
    17521 tgcattcggc ctgcgtgcgc tgcaccagat cggtcgcatc cagacggccg aggtccgccg
    17581 ttccggcgtg attgatgtac caactgctgc cgtgagcgat cgttcccagc tcgatgaagc
    17641 ccgcgggtac caatgggtac ttctttcttg ctttgcggat ggctgcctca atgtcgtcga
    17701 atcgagtctt cgccttgtcg atgttcgtca gcgccagtga caatgtcatg ggcatgagct
    17761 gctcgggatc gatcatgact tccgccccgg cgtagtaggc cacgtctgcg tctcccgtgc
    17821 agtccaccac gacgctcgcg cgaaccgcca tcaatccccg cttggtggca atgaccacgc
    17881 cggcgacgcg gttgtgctcg acgatcgcat cggccgcgcg aacgtgaacc agatagcgga
    17941 caccgaccgc gtccagcgca tcgaggagcg cgaccttcag gtattcgacg ttgcaccaca
    18001 actggtgatc gccgatcttc accgcctggt cgccgtaacg gagcaggtta tgcaccagca
    18061 actcgtggat cgcagagcgc ggcttgcccc cgggacgcac ctggttgatc ggcatcccga
    18121 gcgcccacgc cccgacgccg ccgaaaaacg agtggttctc gaccagcagt gtgttggcgc
    18181 ccgcacgggc ggcgcccagc gccgcgccga tcccagccgg cccgccgccc gcgaccagca
    18241 catccactgt actcaaaacc gggtacttcg tcgccggttc agccgggcgc acggcgcttg
    18301 cgctcttccc tgccaacgcc gctccggcca gactcgccgg aaagagccct cgccgggtca
    18361 gaccacgctt tctcatcgca ctcttctccg cagtaaacgg aatgttgacc agacgactat
    18421 acaccgttga acgcaagact tgtggcccgg taacgataac agccaagctg tgcgcccgtc
    18481 atctcggata tacttcaggc gtggtgcggg tgctggtcat tttgagtgtg atcgtgtgct
    18541 cgctccgggc gcagagtctg cagcaggcga tttcggatgt cgaagccggc cggctcgaaa
    18601 acgccgagca ggccctgacg agatttctcg gctctcatcc cgattctgag gaggcccttt
    18661 tctacatggg cctgctgcat tttcgggcgg gtcgcccagc cgcggcgcgc ccgtatctgg
    18721 accgggcagt gaagctctcg cccaccagcg cgccagcatg gaaagtactg ggcttggtga
    18781 ctgcggcggg cggcaacctg caggaagccc tgacgccgtt cgggaaggct tgccaactcg
    18841 ctccgagcga cgaggaagct tgttattact tcgcacgcaa cctctacgct ctgcatcgat
    18901 acgacctcgc ctgcgagccg ttcgcgcaag cgttgcgtgc cgcgcccgaa ggaatgcggt
    18961 cgaaagtcga ccgggctgcg gcgctgaatc tggaagcctt ggggcggtct gccgaggccg
    19021 aacgtcattt tcgagaagcc gtccgtctgc accgcggcga tggtcgaccc aacgaggatc
    19081 cgcacatcga ctacggagcc ttcctgttcc gccagggccg caccgcagaa gccctgcgga
    19141 ctttggagca ggctaccgcc gcactggcat cctcctcgcg cgcgaacacc gaactgggaa
    19201 gggttctgct ggaattgggg agaccggatg cagccgcggt ccagttggaa agagcgatcg
    19261 ggctggagcg aggcaactgg acggcccgct tgctactcgg tagggcttat ctccgactcg
    19321 gtcgaaaaga ggaaggcaac gaacagatgc gactgggcca agaggggtgg gcgagggaac
    19381 actacgattc ttcgatcgtc aagtagcggt cgccggcgac cttttccagc ctttgccgca
    19441 cgccgctcgg ccactcgatc tcgatcgatt gcgcgcctgc atccttgccc aggccaaaga
    19501 ataccgtccg gtcgctcgcg catccgtagc ccgtggatgt agtgacgcgg ttccactgcc
    19561 cgcgcccaga ggagccgatg acatgaacca gagcgccgag accgtctcgg ttgctgcggt
    19621 ggccgcggag gcgtagggcg agccagtgat ttcctgaggt cgaggtgttg cgaaacagtt
    19681 ccgctcgctc gtcaatccgg cttactacgg cgtccaccct gccgtctcgg tcgaagtcgc
    19741 cgaacgccac gccccgatgg aaccccgtct gctgaaaatc aggcccggcc cgggtgctga
    19801 catcgagaaa gcgcccgccg cccaggttca ccagcaccat gttcgcctga cgcgattttc
    19861 gcggcgagaa catctcagtg ttgtcgtcga ttgagctgca ggctacaaac agatccttca
    19921 gcccgtcgtt gttgagatcg aaaaccccat tgctccaacc ggtggccgtc agggtctggc
    19981 gccccagccc gctgatataa gtgatatcag cgaacagacc cttgccgagg ctgcggaaca
    20041 gaggaaacgt ctcattggct agggctgcga tgaacacgtc ctcgcggcca tcgttgtcga
    20101 tgtcgcgcgc atcgactccc atcgaactga gagcccggcc atcttcgttg aaggcaaggc
    20161 ccgcccagag agccacctcc cgaaatcggc cgccgccctc gttccgaaac aggaagttcg
    20221 gatacgtgtc gttggcgacg aagacgtcga gcctgccatc ctggtcgaag tccaggaagc
    20281 tgacgcccat tcccttgccg atatgcgcgg cgattccgga ggactccgaa acgtcagtga
    20341 acgtgccgtc tccgttattg tggtacaggc tgttcggcag gccggcgtag tacttggggc
    20401 tgcaatatgt gcgaaccccg tttacgctgc aggggggctc cttgccgggg atccacacac
    20461 agtagttcac cacgaacaga tcgagccatc cgtcactgtc gtagtcaaac cagcccgccg
    20521 cgatcgacca cggtttgcgg cgatcggagt tgtgagtcag ccccgcccgt gccgtgacgt
    20581 cttcgaacgt gccgtcgcgc cggttccggt acaaaatgtt gcggttcacg ccgacgatga
    20641 acagatcggt gaagccatcg ctgtcgtagt caccggtggc aacgccggtg gcgaagcctt
    20701 cgcccttcac ccccgcctgt aacgtgatgt ccgtgaacgt gccgtcgccg ttgttacgat
    20761 aaagccggtt gtagaaagca gcagtgtcct tctcgagttt cggttgccgc gctccgttga
    20821 tgaagtaaat gtcgggccag ccgtcgctgt tgtaatcaaa aacggcgaca ccggacacca
    20881 tggtctcgat ctggtgtttc tccggggtgg ccgaattgcg caggatgaag tcgacgcgtg
    20941 cgtgtgacgc gacgttctgg aaagttatcg gatccgggcc ggccggcgca gacgggagcc
    21001 agccggccag gatcgccagc gcggcgcagc cgctggcaac acgaaaacag tctcggatga
    21061 actgaaattg catcactcag tcgagccaca cgaccgcgcc cttgttcacc tctgggatca
    21121 cgcaggtcaa cgatccgttc ttccattcgc ccgcgagttt ctcgacttca ccgaggaacg
    21181 gcagaacgta cacggagcct tttcggccgg ttttgaaggt gatccgtaca ttgcgctccg
    21241 gcagttgctg tgccacctgg ctcgccttca gtcccttgaa attcgccagg aacacgcggg
    21301 tcttcccatc cacctgtgca atttgcgtag aaatgaatgg cgaggtctcc acctctaccg
    21361 ctggtttgat ctgcgacatc ccgagaagtt tggcggccca agcggttctc agacgctcga
    21421 actgcgcctg ctggtattct ccggaggctg ccagttcgtt gaattccttc cgcagttgcg
    21481 cgtagtactc agccccgggg cagcgcggat agtacaagaa cttcctgcgg gaagttcctg
    21541 ctccctcctg ggcgggatcg gcgatgccca gcagcttgtg gatggggttg gccggctgtt
    21601 ccgcgccggt gtcgtcgtat ttaccggtct cgccagtcac gatcagggtc attcccgagt
    21661 tgaaacggga gcgcagaaca tccagttcct gttttccgag gcagcgggcc tcgggcagga
    21721 tgagcacgct gccatggaat gcatccaggg ttcgcggagt caccacctga aattccaggt
    21781 gcgattgcat gaggagcatc atggtgcccc ggtaggagtc gatgaattcc tttgagaagt
    21841 aattgcggct tttcggggag aaatagacac caatcgggcg aatcggcatt ctcggcagga
    21901 agaacacctt ctcgtgatcc cggatccatt ggaagatcac ttttctggtc tcgatgtcgt
    21961 tcgagcctga catgacgtgc ccacgcgcgt cccacgtatt ggtgccggcc atcaactgcg
    22021 ccgtggccag attcttcatc gcttcgcccg gaacgatctc cttttcgttc tcccacgagt
    22081 aggtcagcat ccaggtcggc ttgctgcctg caaacgcccg gaacgaatac atcccgacca
    22141 tgtcctggaa ccaggtcagc ggcgtgcgcc gggcggcccg gccaccggtg ctgtactcgt
    22201 gcgcgatcgt gtctacaacc cgatagaggt cgtagacatc cgtgccgacg cggaccgcgg
    22261 ggtcctcgat gcctggataa atctccgcga tcgtcttgca cttcgggttc acggacttga
    22321 ctgcggcatc gatctgcctc atgaaatcgg tcaaggcgtc gatccgaaaa tcgatccact
    22381 tgcggaaatt ggcgtcgcgg aaatcgccga gcttcagatc agttttggca ttcagtcccg
    22441 tcttccgttt gaatgcggcc accgtgtaat cgtcgaagct cacccaggaa tccccccatc
    22501 ccttgaagtg ggtcatccag tacgggatgt caacgtagac gccgtcgatt ccggtagcgg
    22561 cgatctgccg cacgcgttcc aggtaggtct tcatccagtc ggtcgcaaac ggagtgagcc
    22621 agacgtcctc atcgccttct cgcacccaga aggctgcccc gccgccaaac acggcaggtt
    22681 cgccagtgat cttccgctgc agccagtcag gatggtcctt tgccatcgag tgagcttggg
    22741 aggcggcctt cgccgtgatg cactcggttc ctgcgatata gacgaacgcg tagttgccgg
    22801 cctcgtgcgc tttcgcggcg accgcccgca aggcagtgag cttctcagtg gggtcgagaa
    22861 agctttcata acggccgggg atatcgttgt ccacctcgat gccgaacacg aacgtgtcgg
    22921 tagcgcttcg gacgatctgg tcgacattgc tgagacttac tccatgcccg ccgattcgaa
    22981 cgaagtgcgt ccagtcctga cggtcctggg accggcccgc cgccagcaga aggaacgcca
    23041 acgcaaagag ctttaccgtg cgcatacagc cgatcctcct tgatgtgtgg ggcaaaagcg
    23101 gacactaccc gtcgccccac ggcagtatac tgcaaaagca gtaagcgcat aaccaggagg
    23161 tgtgtgcatg aagcgacgag ctttcttccg gaccgcagga ctggccatcc ccatcgcggt
    23221 caaaccgtcg gccgcttatg ttgtctccca caattgggac aagtacgatt tcggctcggg
    23281 cccgcccgtc aaggagcgac tctatcaggg gccgtttccc caataccctc ctgaacagat
    23341 ctttccgggt ggggacgtgg tgatggcgac cacgccgtcc gatgaagtag tgccgaattt
    23401 cggccgcggg ctgattacgt acatcgctgc ggatatgggg acagccgaaa ttcttggcga
    23461 agacaaggcc aaagctatcg aggaactcgt caagctcccg ctcggccaaa agctctacat
    23521 ccgtccgacg tggcgcgagg tccagccccg ccgcggccgc ctcgagtttc ccgattactg
    23581 gaaactcacc ttcgatctag ccaagcaata caacaagcaa gtaggtttcc gaatccagat
    23641 gcgcgctccg gactatccgg aggaagccct gccggacttc gtcctggaga aagtgcccat
    23701 ggtgaaactg gaagggcagt ggagacggaa cagcaagcgt gaattccagg agccgcgcta
    23761 cgaccatccg gcattccagg aggcttttca agagctcaac gggctgttgg cagcggaact
    23821 gaacggcaac ccgctcgtcg agttcgtaga cacgttcatg tatggcttct ggggcgaggg
    23881 ccacacctgg cctttccgga acaatccgtt tccgaattac cagacagcgg agcgaacctg
    23941 ggccggcatg tttgagaagc aactggagca gtggacaaag acgccgctgg ctaccaatac
    24001 gcagcccgat ttcagcctgg tgggcaattc cgagctcgtc gatcgcacgg ttcgcagcta
    24061 caactggctg cgcaccgaca ccatcttcat cgagaacgta cagatcgagg cgatcggcaa
    24121 tcgtccgcca tgggtggcgg cggttctcga agtcggaatg tccgacggat cacccaactc
    24181 gctgcgcatc agtgaagggg tgccggcaac tgacaacatg atccaacacg tgctcgacgt
    24241 gggcgcgaac tactggtcgc tgtggaattt ccaccggatc agcgcggaag gcattctgaa
    24301 ttactaccgc cagtacccga aaaagatcga cgaaatcaac cgcagtatcg gctacaatgt
    24361 gcggccttcg ttcgtctggg tttacgaggg cgataatccc ggactgatta tcgggttcgc
    24421 gaacgatgga gttgccggcg tgcccggggt gttacgggtt actgtcttcg gcgaagacgg
    24481 caaagcgtac gcatccggcg ggttagacgc gggatatcct ttgcctggca agatccggca
    24541 ggcccagttc ccgctgccga aaggcacgaa atgggaaggg ttgcaactta aggcggaact
    24601 ggaagtgaaa ggggtgcggc acccggtccg atgggcgtgt catcagaaga cgaaccctga
    24661 tggctcactg acccttcgcc gcaacctcgg gcggggatag tttccccgct cacgggatcg
    24721 tcggacttgc gagccacgaa cagctatgaa tacaccatcc ttgcgcattg ctctttgctt
    24781 cgtcgcgggc atcgccgcgc ttgcacagcc gccctatgat ctgctgctga agggcgggca
    24841 cctcatcgat cccaagaacc gggtcgacgc aattcgcgac gtcgcgatct cgagcgggaa
    24901 gatcgcggca gtggccgaga agatcgagcc cgcgaaagcg cgccgggtcg tcgacgtgag
    24961 cgggttgtat gtgaccccag gcctagtcga cattcatgtc catgcgtttg ccggcacgat
    25021 ggcgccggag tacacaggag cgctgagtgt ccggccggac gacttcacgt tccgcaatgg
    25081 cgtgacgacc gtcgtggacg cgggcagttc cggctggcgc aacttcgcgg aattcaagca
    25141 gcagatcatc gataattccc gcacgcgcgt gctggcgatg ctcaacattg tcggccgggg
    25201 catggcgggg cgcacgacgg tcgagcagga taccaccgac atggatccgg agcggaccgc
    25261 ctccgaggct gcgcgctatc cagggctcat cgtcggcatc aagatcgccc actatgccgg
    25321 tccggaatgg accgctgtcg aacgggcggt tgaggctggc aggcgggcgg atcttcccgt
    25381 gatggttgac ttcgcggaat tccgccccga gcggccgttt caggacctgg tcttgaggaa
    25441 gctgcggcct ggagacattt acacacacct gtatttgggc gcggtgccca tgctcgaccc
    25501 gcaagggcga gtgttgcctt acctgcttga ggcaaggaaa cggggagtga tctttgatgt
    25561 cgggcatggc gcaggaagtt tcctattccg gcaggcggtt cccgccgtcc ggcagggtct
    25621 tgttcccgat tcgatctcga ccgacctcca tgtgagcagc atgaacgcag gcatgaagga
    25681 catgctcaac gtcatgtcga agttcctcaa tatgggcatg tcgctggagg acgtgattct
    25741 gcgctctacc tggaaccctg cgcgggagat ccgccacccg gaactcgggc acgtctcggt
    25801 cggatcctcg gccgatatcg ccgttctgcg aattgtggat ggcaattttg gcttcgtgga
    25861 ttccagccat agacgcctcc agggcacgaa gaggctggtt gcggagctca cgctgcgcga
    25921 cggcagagtg gtctgggatt tgaacggcct cgccagcgac gactgggcca agtagctgcg
    25981 gctctaattc aacccggctt cgaactcgat tcgctgccct gtcaccaggc ggtcgcactc
    26041 gattgtgagc ctgggattct tgttgcccga ggcgaccatg aactgacggg tctc
//
LOCUS       DATIFQ010000498         4996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1702134, whole genome shotgun sequence.
ACCESSION   DATIFQ010000498 DATIFQ010000000
VERSION     DATIFQ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702134"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VLE22_25035"
     CDS             complement(<1..1026)
                     /locus_tag="VLE22_25035"
                     /inference="COORDINATES: protein motif:HMM:NF026034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HSB17738.1"
                     /translation="MFHTPRPRWLRPSLFTLFGALLIGAWLVHTGQSVQAPAPARPDP
                     NVIWRIGQGDGLSDEFGLAPADDLTYTVGKSTPREGWRHRQIARAANPASYRVRFTLD
                     AVPQHGAALDAGLFFVESAPHAILLDVNGKRGSFRLRSAAAEDTDERQANSILHSRQS
                     LRMPIPAALLRKGENEIAISLVGDTGAIEYDSLALLKAAAPLPKLDAAVEPTIFFRRH
                     AGQLAELTEVVLHHTAPIGKVQVSLTAGGTAVSSVSEDTGHDFGERVIELDVPVPSGP
                     APYEVTVVSTGKPVTFRGDFRPEKQWKIFAGLKIHNDIGYTDLQPHVQELDNRNTDGV
                     LDIIARSP"
     gene            1169..1489
                     /locus_tag="VLE22_25040"
     CDS             1169..1489
                     /locus_tag="VLE22_25040"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HSB17739.1"
                     /translation="MDLPSGVEEMVRLIADRFSPEKIILFGSHARGDSGRDSDIDLLV
                     LFREVDNPRQRAAELYAAVAGTTLPKDIVVSTTSRLERFRNVANTVYWPAAREGRVLY
                     ERAA"
     gene            1473..1886
                     /locus_tag="VLE22_25045"
                     /pseudo
     CDS             1473..1886
                     /locus_tag="VLE22_25045"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
     gene            2379..2711
                     /locus_tag="VLE22_25050"
     CDS             2379..2711
                     /locus_tag="VLE22_25050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17740.1"
                     /translation="MENGIGATKRPLVAIFVVLILAVVLLGTNIYFIVEERQRRQAVA
                     DSVLKSIADLEKQRVEVFSDYVRDLASWETKSVYHQIYHANNAQLKLQNLLVQENQLI
                     GVLIAGKK"
     gene            complement(2922..3209)
                     /locus_tag="VLE22_25055"
     CDS             complement(2922..3209)
                     /locus_tag="VLE22_25055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17741.1"
                     /translation="MPSDSSKLPFEYQYLISFHRAPLVLALDQYYDLGPGEQRQFGVD
                     IDGVFATLASDTALVGSVKTGEAVCAPAEPFANLELKLGGGQFDWNCRVPW"
     gene            3508..3657
                     /locus_tag="VLE22_25060"
     CDS             3508..3657
                     /locus_tag="VLE22_25060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17742.1"
                     /translation="MPDINPSEPEQAYSEDGKGSQVTGPLVTGPGKLGPRFPTDENPH
                     DLVWS"
     gene            complement(3671..4405)
                     /locus_tag="VLE22_25065"
     CDS             complement(3671..4405)
                     /locus_tag="VLE22_25065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17743.1"
                     /translation="MTDSAIKQIAAWSVRVYALILHVYPEGFRLEFGESMTQVFRDLA
                     RDAGKRSGLTGLTALWMLTFADVLASVAGAYSSERRKSMFRVVIAAGILYVCTLALVT
                     GYGAIRFGEFYERPAFSGFRAAEPVNEDKLTAAYEQALTGDFGRYKTFAVGAGLVLAV
                     LLGVASALFGLWQKSLLRSAGAFVAGSVLTIVAVSLLPTIWFPLDRYPVGFVWMLRCF
                     PVAAGAWLLVTVIGRFGPGRARFKLA"
     gene            complement(4402..4785)
                     /locus_tag="VLE22_25070"
     CDS             complement(4402..4785)
                     /locus_tag="VLE22_25070"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSB17744.1"
                     /translation="MVKRNMRNAQAAPVTPAEFYILLALADEDRHGYAVMQQVASDSA
                     GSVRLGPGTLYTAIKRLLDYGYIREVESRFDPDLDDARRKYYRLTPSGHAAANAEAER
                     LAGLVRLARTRQIINAQPRAQGGRS"
BASE COUNT         1058 a         1420 c         1587 g          931 t
ORIGIN      
        1 cggagagcgg gcgatgatgt cgaggacgcc gtccgtattg cggttgtcga gttcctgaac
       61 gtgcggctga aggtcggtgt agccgatatc gttgtggatc ttcaggccgg cgaagatttt
      121 ccactgcttc tcagggcgga aatccccgcg gaaggtcacg ggtttgcccg tggaaacgac
      181 ggtgacttcg tagggcgcgg gtccggaggg gacagggacg tcgagttcta tgacgcgctc
      241 gccgaagtcg tggccggtgt cctcgctcac ggaggagacg gccgtgccgc cagcggtgag
      301 gctgacttgg accttgccga taggcgctgt gtgatgcagg acgacctcgg taagctccgc
      361 gagttgacca gcgtggcggc ggaagaagat ggtcggttcg acggcggcgt cgagtttcgg
      421 gagtggcgcg gccgccttga gcagagcgag cgagtcgtat tcgatggcgc cggtatcgcc
      481 cacaagcgaa atggcgattt cgttctcgcc ctttcgaaga agtgcggcag ggatgggcat
      541 tcggagggac tggcgggaat ggaggataga attggcctgc cgctcgtcgg tatcttcggc
      601 ggcggcgctg cgaaggcgga aggatccgcg cttgccgttt acgtcgagca ggatcgcgtg
      661 cggcgcggat tcgacgaaga agaggccggc atctagcgca gcaccgtgct gagggacggc
      721 gtccagtgtg aagcggacgc gataggaggc aggattggct gcgcgggcga tctgccggtg
      781 acgccagcct tccctgggcg tgctcttgcc cacggtgtag gtgagatcgt ccgctggggc
      841 gaggccaaat tcatcggaaa ggccgtcgcc ctggccgatg cgccatatga cgttcggatc
      901 gggccgcgcg ggcgcgggag cctggacgga ctggcctgtg tggacgagcc acgcgccgat
      961 gagaagtgcg ccgaacaagg tgaacaggct cgggcggagc cagcggggtc gcggcgtgtg
     1021 gaacatctcc gaacctccag tcggcccgtg cggcggcgaa ccggccggcg ggcttcatgg
     1081 aatgatacac ggaattcagc gggaaacaag cgggaagcgt gagccaatcc aaggaaacgg
     1141 gttatcatca gcagagatga gtcgactgat ggatcttccg agcggtgttg aggagatggt
     1201 gcgcctgatc gcggaccgct tctcgccgga gaaaatcatc ctgttcggat cacacgcccg
     1261 cggtgactcc ggcagggaca gcgacataga tttgctcgtc ctgttccgtg aggtcgacaa
     1321 tcctcggcaa cgggcggcgg agttgtacgc agccgtagcc gggacgacac tgccgaagga
     1381 catcgtagtg tcgaccacgt ctcgcctcga acgattcaga aacgttgcca acacagtcta
     1441 ttggccggcc gcccgcgagg gcagagtcct ctatgagcgt gccgcctgaa gtcgttgagg
     1501 tacttcgcca gtgggttcga aaggcggagc acgatctgga ggccgctcgg cggatcatga
     1561 ccgtggacga aggctgcccg tatgacacgg cctgttttca ctgccagcag attgcggaaa
     1621 agtatctgaa ggcccttctt actttggcga acatttaggc gccccgcact catgacctgg
     1681 aggcgctggc aactatgctt ccgtcagatc gacgcttgtt ggtgccggcg gcggatctcg
     1741 cgacctcaaa tccgtatgtg gtggacgtgc gatacgcaga cgattggcgt gaagtccaac
     1801 gtgacgacgc gattcagtgc ctgaaactgg cagaaacggt cagggcagaa gttcgggcgc
     1861 tgctgcctcc ctcggccctc gtctgaaggc gggcaactcc agctcctaag acgaggaaga
     1921 aaggattaag gcccggcgtg cccaccaacc tgcacagcat cccgaccgtc gcgcacaacg
     1981 gtctagtggg gggtgtaggc tcagtctgcg gtggcgtttc gcttttcgtc gatagttaac
     2041 ccgacggagt aaaccgcgtg gcgacacagg ccaaccaacg agtcttcgca gacccgttgg
     2101 attgtaccga cgacggggtc aactccccag tacagttcct gcgaaagatg tctcgcgagc
     2161 tcatcggggc tcgtcgcgac catcggtcgg gctatgaatg gaacctcggc acagaggaag
     2221 cggccagagt caaggcccta gtcctcccgt ttcgccgctg gcgacttcat gtagcaccga
     2281 cctacgcccg atctttggag gctgggacga gcaggagtta gacacaactt ggacatttgg
     2341 cggtaaactg gcacagcggt caggagagga gagaatcaat ggagaacgga atcggagcga
     2401 caaagcggcc ccttgtggcc atcttcgtcg ttctcatctt agctgttgtg ctacttggaa
     2461 caaacatcta tttcattgtg gaagagcgtc aacggcgaca agctgtggcc gatagtgtac
     2521 taaagagcat tgccgatctt gagaagcagc gggtagaagt attctccgat tatgtcaggg
     2581 acctcgcgtc gtgggaaacg aagtcagtct atcatcaaat ctaccacgcc aataatgctc
     2641 agcttaagct gcagaaccta ctggtacaag agaaccagct gatcggtgtc ctcatcgctg
     2701 ggaagaagtg agactgtgtc ctagtcgatt tcgggttagc agtgtcaagt ctgatgtgaa
     2761 cgattcgagt ataggaaagc gcaatctttt cgttaattac tgatgaactg ctgattccct
     2821 gctcgagaaa cgcaatactg gcgtaatggc gaagctcttc acctctccag ctgtgattga
     2881 atagaacctg ggctcaggaa gaaatggagc gaaacccgaa ctcaccatgg aacccggcaa
     2941 ttccaatcga actgtccccc gccgagtttg agctccaagt tcgcgaatgg ctctgccggg
     3001 gcgcagaccg cctcaccggt tttcacgcta cccaccaagg ctgtgtcact ggccagggtg
     3061 gcgaatactc cgtcgatatc cacgccgaat tgacgctgtt ctccggggcc cagatcatag
     3121 tattggtcga gtgcaagaac cagcggcgcc cggtggaacg agatgaggta ctgatactcg
     3181 aagggaagct tcgagctgtc ggagggcaca agggaatgtt gttttcaacg tcaggcttcc
     3241 agaaaggcgc aatcctgctg gcaggtgcgc gaggtatcgc aactgtgacc gtccttgacg
     3301 gccgttggtt gtacgaaacg agatccgcag ggcctcctcc tccgccgcca tggataaagt
     3361 tgccacgttt tgccggcgag ataatgtcgc ccaccgaggg cggctggtta tctgctcgca
     3421 tagacgacga gcaggtagac ggcatcgtca gatacctcga aggccgcacc tagcggatcg
     3481 gtgagtgttg tatcgtgaag aacagaaatg cccgatatca atccctccga gcccgaacag
     3541 gcctacagtg aggacggcaa aggctcccag gtgaccggcc cgctcgttac agggccgggc
     3601 aagctgggtc cacgctttcc gacagacgaa aaccctcacg acctcgtctg gtcctgaatg
     3661 tccggctccg ttacgccagt ttgaaccggg cccgacctgg cccaaagcgc ccgatcacgg
     3721 tcaccagcag ccatgcgcct gccgcgacgg gaaaacatcg gagcatccag acaaacccca
     3781 cggggtagcg atccagcgga aaccagatcg tcggcagtaa cgagaccgca acgatggtca
     3841 gcaccgagcc ggcgacgaac gcacccgcgc ttctcagaag gctcttctgc cagaggccga
     3901 acagcgcgga agcgacaccc aacaagaccg cgagcacgag cccggcgcca accgcaaatg
     3961 tcttgtagcg gccgaagtcg cccgtcagcg cctgttcgta tgccgcagtc aacttgtcct
     4021 cgttcacagg ctccgctgcg cggaacccgc tgaaggcggg ccgttcatag aactcgccaa
     4081 atcggatcgc accgtagccc gtcaccaggg ccagtgtgca cacgtaaaga atacccgcgg
     4141 cgataaccac cctgaacatc gacttcctcc gttcgctcga gtacgcgccg gcgacgctgg
     4201 cgagcacgtc ggcgaaggtc agcatccaca gcgcggtcag gccggtaaga cccgaccttt
     4261 taccggcgtc acgggccagg tcacgaaaaa cctgtgtcat cgactcccca aactcaagcc
     4321 ggaatccttc ggggtacaca tgaagaatga gcgcatagac acgcaccgac cacgcagcga
     4381 tttgctttat cgccgagtcg gtcatgatcg gcccccctgc gctctcggct gagcattgat
     4441 aatctgcctg gtgcgcgcca gcctcaccag acctgccagc cgttcggctt ccgcgttcgc
     4501 agccgcgtgg ccggagggcg tcaaccggta gtatttgcgc cgggcgtcgt cgaggtcagg
     4561 atcgaagcgc gattcgacct cgcggatata cccgtaatcc agcaggcgct tgatcgcggt
     4621 gtacagcgtg ccggggccga gtcgcaccga gcccgcactg tcggaggcaa cctgttgcat
     4681 cacggcatag ccgtgccggt cctcgtccgc cagcgcgagc aggatgtaaa actccgccgg
     4741 cgtaacggga gcggcttggg cattgcgcat atttcgtttg accatgacta tatcgactga
     4801 cgatatatgc gataccgcca gtgcaactgc atgtcaagac caagagcggg cggattgtcg
     4861 ttaacatgtg atctgtccgg tggaattcac agatgatctg tccgctttga ggattgcggt
     4921 ctgagaggct ggctgggtga gggagacccg gcagtactcg gccgccgaga tggagcggat
     4981 gatgaaactg caagac
//
LOCUS       DATIFQ010000499         5257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_938627, whole genome shotgun sequence.
ACCESSION   DATIFQ010000499 DATIFQ010000000
VERSION     DATIFQ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938627"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VLE22_25075"
     CDS             complement(<1..309)
                     /locus_tag="VLE22_25075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17745.1"
                     /translation="MAYVLRFVQRFRVSEQAAFLELENQFAELERRSEGWPRGRRMRP
                     LSGREPGNTLIWECEFPTLEDVHAAIRTMDASPDHARLFAMQSPMMLDAWTEIHEVLC
                     E"
     gene            394..1896
                     /locus_tag="VLE22_25080"
     CDS             394..1896
                     /locus_tag="VLE22_25080"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSB17746.1"
                     /translation="MNFAPIDWIIVVVYLAASLIMGVIGKRYVGSITDYLVAGRELGP
                     YVGIATLAATEIGTITFMYYAELGYKYGFAAFAAAVISGVVMIIVGRTGLIISRFREL
                     KLMTVPEFFERKYSPGLRILTGILVATGGILNMGVFLKVEGEFLTIVTGIDPRYLVTV
                     MTVILLLELTYTVLGGMVSVVITDFIQYALLSVAVILVSIWAVLHTGWDSMVTKVVTT
                     MGHDGFSPFTNPRFGVTFLIWQLLLWFALHTCWQTTAMRMFSTRSPEISKKVMTWTGF
                     IFLGRGMLPILWGIAALAMLGTGPAGKDGVLLPVVGGQVLQPVEAMPAMLARILGPGV
                     RGIVVAGMLAATMSVNSSYLLGWSSVISQDIILPVRRRLGKGPLSSRQQILLNRLANL
                     FVSLFVMFWGLYYTLPGAVYLYLHITATIYLAGAFTCAVGGLYWRRANVAGGYAAMLL
                     GAAGAIVPYFFLHWGENVTGFAAFGLAALGFVAGSLLWKRGDAALEVPHA"
     gene            1889..2032
                     /locus_tag="VLE22_25085"
     CDS             1889..2032
                     /locus_tag="VLE22_25085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17747.1"
                     /translation="MRSWLTFWTLWLLVAGASFAAITVVVIVKGARDLRDMFTGLKRS
                     REE"
     gene            complement(2042..3331)
                     /locus_tag="VLE22_25090"
     CDS             complement(2042..3331)
                     /locus_tag="VLE22_25090"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HSB17748.1"
                     /translation="MRARRALGMYLLALLVAPCLRAGGGFRASVVKVDITPDRPQWLL
                     GYGARQSTSVHDRIYHRIVAMDDGKTQFFLISSDICLISPSEYDKVTGELEKRTGIQP
                     LNVWWTFTHTHSAPELGPPGLPAVFMGNRYKHEGDTEYWAWVEQRLIDGVIEARSKLE
                     PARIGAGWGLSMANINRRARDVEGEAFLGLNPDGPVDRRIGILRIDRADGKPLVLIAN
                     YAMHGTVLGGESLVISGDAPGVVSEYVEQKGGAPMLFINGAAGNVAPIYTVQRDPKSG
                     RLGQFRVLLGDRILAANRTITATSSEVTLKLGSLTVETPRKAGLGWAPDLGDYTRTDA
                     AGTALVRLPVRFLRINDDIAIWSAPLELFCEVAMEIRDRSPYPYTFYYGYTNGWLGYL
                     VTEEELKYGGYEPSVSPYTAQAASDLTGAVTSYLDSR"
     gene            complement(3343..>5257)
                     /locus_tag="VLE22_25095"
     CDS             complement(3343..>5257)
                     /locus_tag="VLE22_25095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HSB17749.1"
                     /translation="LGGDMSDPTATGRRGFLKMTAMAGGLAASQSAPAGQTDQQAQTA
                     QSIHETKYEDRTDSVEEKLRLLAAAYRKRDYRLSRALAASLRETLQFEQHDQESSGEA
                     VIAPDTFGAVGDMPEPWRRWARTWRYYKVLDIQETAGAERSGEPVEALIGFRGDQADS
                     LRREVRIARVEPDGGLREVACQVSGDVRRETTLFARVQFLAHVLASGRTTYLVFYGNP
                     DAELPSYPSDLTVSGEGFGLDIENDYYVASLSRQMGQLERLKLKREHGLELFAGGEGH
                     GEPPGIDWAHDYVASNNFQKFRITNWPTCPDYEVIRGPLCVTVRRWGFPNSTIHPLFT
                     PSRMNIFVEYRFYAGAPYFIKEGSMEVIKDMEITYLRDDEWVFSGHSFTDTLWMGRNG
                     KLRTGPVDAAAQDDLWATGFFHRVSRDAFLALFLKHEAEGVDGLKHTGAPQLYYKWHG
                     PLWSRALFQNARMPKGSVLRQKNAYLVLPFPEQGGAEMAEGWRQRLINPLVLRGGDLP
                     SDASATPGSLARPGEAADSVIDKQRIWEALADCKDYQLYTADVSVVDLGLIYDVRVRG
                     ETVHVVMTMPHRGRPRHGYFAWGSGGNLQPIRDRLLKLPGVRKVVVEPVWEPAWSSNR
                     VTEKGRRALGLPD"
BASE COUNT          879 a         1727 c         1607 g         1044 t
ORIGIN      
        1 ctcacacaaa acctcgtgga tctcggtcca cgcgtcgagc atcatcggcg attgcatcgc
       61 gaacagcctc gcgtggtcgg gcgacgcatc catcgtgcga atggctgcat ggacatcctc
      121 cagcgtcggg aactcgcatt cccagatgag cgtattgccc ggctcgcggc cgctcagcgg
      181 cctcatgcgc cgcccgcgag gccatccttc gctgcgccgc tccagctcgg cgaactggtt
      241 ttctaattcg agaaaggccg cttgctcgga caccctgaat cgctgcacga agcggagcac
      301 ataagccacg actgtttacc tccggcggag acaaggctaa caaactccgg gatagaatgg
      361 gtgcctgctt gtcccggccg gaggaccggg gggatgaatt tcgctccaat tgactggatc
      421 atcgtcgttg tctatctcgc cgcctcgctg atcatgggcg tgatcggcaa gcgttacgtc
      481 ggctcgatca ccgactacct cgtggccggg cgcgagctcg gcccgtacgt cggtatcgcc
      541 acgctggcgg cgacagagat cggcaccatc acgttcatgt actacgccga actcggatac
      601 aagtacggct ttgcggcttt cgcagcggcg gtcatctccg gcgtggtgat gataatcgtg
      661 ggccgcacgg ggctcatcat cagccgcttc cgcgaactca agctgatgac cgttcccgag
      721 ttcttcgaga ggaagtacag cccgggcctc cgcatcctca ccggcatcct ggtggccacc
      781 ggcggcattc tcaacatggg cgtgttcctg aaggtggagg gcgagttcct cactatcgtc
      841 accggtatcg acccgcgcta cctggtcacg gtcatgacgg tgatcctgct gctcgagctg
      901 acttacaccg ttctgggcgg catggtctcc gtcgttatca ccgatttcat tcagtacgcg
      961 ctgttgtcgg tggccgtgat cctggtctcg atctgggccg tgcttcacac cggatgggac
     1021 agcatggtga ccaaagtcgt caccacaatg ggccacgacg gcttcagccc gttcaccaat
     1081 ccacggttcg gcgtcacctt cctcatttgg cagttgttgc tttggttcgc tcttcacacg
     1141 tgctggcaga ccacggccat gcggatgttc tcgactcgca gtccggagat ctcgaagaag
     1201 gtcatgacgt ggaccgggtt catcttcctc ggccgcggca tgctccctat tctctggggt
     1261 atcgccgcgc ttgcaatgct cgggacggga ccggcgggga aagacggcgt gctgctgcca
     1321 gtggtgggcg ggcaggtcct acagcccgtt gaagccatgc ccgcgatgct cgcccgcatt
     1381 ctcgggcctg gcgttcgcgg catcgtggtg gccggtatgc tcgccgccac catgtctgtt
     1441 aacagttctt atctgctcgg atggagttcg gtgatctcgc aggacatcat cctgccggtg
     1501 cggcggcgac tcggcaaggg accgctcagc tcccggcagc aaatcctgct caaccgcctg
     1561 gcgaacctct tcgtcagcct gttcgtgatg ttctgggggc tctactatac gcttcccggc
     1621 gcggtttacc tctatctgca tatcaccgcg accatctact tggcaggcgc atttacttgc
     1681 gccgtcggcg gcctgtactg gaggcgcgcg aacgtcgcgg gaggatacgc ggcgatgctc
     1741 cttggggcgg cgggagccat cgtgccgtac ttcttcctgc actggggcga gaacgtgacg
     1801 ggcttcgccg ccttcggact cgcagcgctc gggtttgtcg ctggttccct gctctggaaa
     1861 cgcggcgacg ccgcgctgga ggttccacat gcgtagctgg ctcacgtttt ggacgctgtg
     1921 gctgctggtg gcgggcgcgt cgttcgcagc cattaccgtc gtcgtcatcg tgaagggcgc
     1981 tcgcgacctg cgcgacatgt tcaccggtct caagcggagc cgggaggaat gaaccggagc
     2041 gctatcggct gtcgagatag ctcgtcaccg cgccggtgag gtcgctcgcg gcctgtgcgg
     2101 tgtacggtga gacactcggc tcgtagcctc catacttgag ctcctcttcc gtcacaaggt
     2161 agccgagcca gccgttggtg taaccgtagt agaacgtgta cggatacggc gagcggtccc
     2221 ggatctccat cgcaacttcg cagaacagct ccagcggtgc actccagatt gcgatgtcgt
     2281 cattgatcct cagaaaccgg acgggcaggc ggaccagtgc cgtccccgcg gcatcggtgc
     2341 gggtgtaatc accgaggtca ggcgcccagc caagcccggc tttccgcggc gtctcaaccg
     2401 tcagagaacc gagcttgagg gttacctcgg aagaagtcgc cgtaatcgtg cggttcgctg
     2461 ccaggatccg gtcgccgagc aacacacgga actggccgag ccgcccgctc ttcgggtcac
     2521 gctggacggt gtagatcggc gccacgtttc ccgcggcgcc gttgatgaac agcatcggtg
     2581 cgccgccttt ctgctcgacg tattcgctaa caacgccggg cgcgtcacca ctgatcacga
     2641 ggctctcgcc gccaagcaca gtgccgtgca tcgcgtagtt cgcgatcagc acgagcggct
     2701 ttccgtcggc acggtcgatc cggaggatcc cgatccgccg atccaccgga ccgtccgggt
     2761 tcagtcccag gaacgcctcg ccctcgacgt cccgggcgcg cctgttgatg ttcgccatcg
     2821 acagacccca gccggcgcca atccgtgccg gttccagctt ggagcgtgcc tcgatcacgc
     2881 cgtcgatcag ccgttgctcg acccaggccc aatattctgt atcgccctcg tgcttgtagc
     2941 ggttgcccat gaaaacggcc gggagtcccg gtggcccgag ttcgggagcc gagtgagtgt
     3001 gcgtgaacgt ccaccagaca ttcagcggct ggatacctgt ccgcttctcg agttcgccag
     3061 tgactttgtc atattccgac ggcgagatca ggcagatatc cgaggagatg aggaagaact
     3121 gcgtcttgcc atcgtccatc gcgacgatgc ggtggtagat acgatcgtgc acgctcgtcg
     3181 actggcgcgc gccgtagcca agcagccact gcggccggtc cggtgtgatg tccaccttga
     3241 ctaccgacgc ccggaagccg ccgcccgcac gcagacacgg cgcaaccaaa agcgccagaa
     3301 gatacattcc caatgctcgt cttgctctca agatcgcctc cttcagtccg gtaaacccag
     3361 cgcacgccgt cccttttccg tgacccggtt ggaagaccag gccggctccc acaccggttc
     3421 caccaccacc ttccgcacgc ccggcaactt gagcagccgg tcgcggattg gctggagatt
     3481 gccccccgat ccccaggcga agtaaccgtg tctcggccgc cctcggtgcg gcatggtcat
     3541 cacaacgtgg acggtttcac cccgcactcg cacatcgtag atcaggccga gatcgaccac
     3601 gctgacgtcg gccgtgtaga gttggtaatc cttgcagtcg gccagcgcct cccagatgcg
     3661 ctgtttgtcg atcacgctgt cagcagcctc gcccggacgg gcgagacttc ccggagtggc
     3721 tgaggcgtcg gatggcaggt cccccccgcg cagcacgagc gggttgatca agcgctgccg
     3781 ccagccctcg gccatttccg cgcctccctg ctcggggaac gggagtacga ggtaggcgtt
     3841 cttctgccgc agcaccgagc ccttgggcat ccgcgcattc tggaagagcg ctcggctcca
     3901 gagcggcccg tgccacttgt aatagagctg cggcgcgccg gtgtgtttca acccatcgac
     3961 tccctccgcc tcatgtttca ggaacagcgc cagaaaggca tcgcggctca cacggtggaa
     4021 gaagccggtc gcccagaggt cgtcttgcgc ggccgcatcc accggtcccg tgcgtagttt
     4081 gccgttgcgg cccatccaga gcgtatcggt gaatgagtgc ccggagaaga cccattcgtc
     4141 gtcgcgcaga taggtgatct ccatatcctt gatcacctcc atgctgcctt ccttgatgaa
     4201 gtacggcgca cccgcgtaga aacggtactc gacgaagatg ttcatccggc tcggtgtgaa
     4261 caacgggtgg atggtcgagt tggggaagcc ccaacggcgc acggtgacgc aaagcggtcc
     4321 gcggatcact tcgtagtctg ggcaggtggg ccagttggtg atccggaact tctggaagtt
     4381 gttcgaggcc acgtaatcgt gtgcccagtc gatgccgggc ggctcgccat gcccttcgcc
     4441 gccggcgaac agctcgaggc catgctcacg cttcagtttc aggcgttcca gttgccccat
     4501 ttgccgggag agggaggcga catagtagtc gttttcgata tccaggccga aaccctcgcc
     4561 cgacaccgtc aggtcgcttg gatagctggg gagttccgcg tccgggttgc catagaagac
     4621 gaggtaggtc gttcgtcccg acgctaggac gtgtgcgaga aactgcacgc gggcgaacag
     4681 cgtcgtttct cgccgcacgt cacctgacac ctgacaagcg acttcacgca gcccgccatc
     4741 cggttcgacg cgcgcgatgc gcacctcgcg gcgaagggag tccgcctggt ctccgcgaaa
     4801 accgatcagc gcctcgactg gttcgccgct ccgctccgcg ccggcggtct cctgaatgtc
     4861 aagcaccttg tagtagcgcc aggtgcgggc ccagcgccgc catggttcgg gcatatcgcc
     4921 aaccgccccg aacgtgtcag gcgcgatgac ggcctctccc gagctctcct ggtcatgctg
     4981 ttcgaactga agcgtctccc gaagcgaagc ggccagcgcg cgcgacagcc ggtagtcccg
     5041 tttccgatac gccgccgcca gaagccgcaa cttctcctcg acactgtccg tccggtcttc
     5101 atacttcgtc tcatggatcg actgcgcggt ctgtgcctgc tggtccgtct ggcccgccgg
     5161 tgcggattgg cttgccgcca ggccgcccgc catcgccgtc atcttgagga atccgcgccg
     5221 tcccgttgcc gtcgggtcgc tcatatcgcc tcccaat
//
LOCUS       DATIFQ010000500         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2663871, whole genome shotgun sequence.
ACCESSION   DATIFQ010000500 DATIFQ010000000
VERSION     DATIFQ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2663871"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2573
                     /locus_tag="VLE22_25100"
     CDS             <1..>2573
                     /locus_tag="VLE22_25100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17750.1"
                     /translation="VDPESLSSFVMSYQKVTVLTLGELWAIPIMLRLALIDNLRRVGA
                     RIVAGSIDRDLAARWADQMLEIAEKDPKSLILLTADMARSDPPMLSSFVAEFARRLQG
                     QSTALALPLTWIEQRLSESGRTIEQLVRSENQQKAADQVSISNSIGSLRFLGAMDWRA
                     FVETMSVVDLVLREDPGGAYGRMDFTTRDRYRHVIEKIAKRSRLHEADVAREAIRMAV
                     DGAARNGPDDRSAHVGFYLIDKGSLRLERAAGVRLPLLETLLRTAGRCPLLLYLGSIT
                     LLAAIFSGALLAKAHAGGLRDWPLALFGALSLLCVSPLAVALVNWFATLLVKPVPLPR
                     MDFSEGIPAESRTLVVVPTMLTSPRGIESLLESLEVRFLANRDENLYFGLLTDFQDAN
                     EESVPEDGELVRLARGGIEELNEKYGGAKGDPFFLFHRPRRWNPQERTWMGHERKRGK
                     IAELNLFLRGGARDRFSIVVGNTANVSGVRFVIPLDTDTQLPRDSARQFVGAMSHPLN
                     RARYDERKQLVAEGHGILQPRVADSLSGTNRSRYARLFGSEPGIDPYTRAVSDVYQDV
                     FDEGSFIGKGIYDVDAFEQALKGRLPENRILSHDLLEGCYARAGLLSDAHLYEEYPSS
                     YIADVNRRHRWIRGDWQIAGWLLSRVPGPGGTRRRNPLSGLSQWKIFDNLRRSLEPSA
                     LTLLLLLGWNSFSSPWVWTFPVLGILLVPSVLASALEMLRKPADTLLRQHLAAEARSA
                     GRRFAQVAVTLACLPYEAYFGLDAVVRTAWRMLFTHRRLLEWRPSGTPDPDRGEGLAA
                     FCRTMWIAPVIAVAAAIHLGVSRPVALATAGPILALWFASPAITWWISRPLARRRAAL
                     SA"
BASE COUNT          405 a          823 c          870 g          475 t
ORIGIN      
        1 gtggacccgg aaagtctcag cagtttcgtg atgtcctacc agaaggtgac cgtcctgacg
       61 ctgggggaat tgtgggccat ccccatcatg ctgcgcctgg ccctgatcga taatctccgg
      121 cgcgtcgggg cgcggattgt cgccggaagc atcgaccggg acctcgccgc ccgctgggcg
      181 gatcagatgc tggagatcgc ggaaaaggat ccgaagagcc tgatattgct gaccgcggac
      241 atggcgcggt cggacccgcc gatgctgagc tcgttcgtcg cggaattcgc ccgccgattg
      301 cagggacaga gcacggccct ggcgttgccg ctcacctgga tcgaacagcg gctttccgag
      361 tccgggcgga cgatcgagca gctggtgcgg tcggagaacc agcaaaaggc cgccgaccag
      421 gtttccatca gcaacagcat cggcagcctc cggttcctgg gggcgatgga ctggcgcgca
      481 ttcgtcgaga cgatgagcgt cgtcgacctg gtgctgcgcg aggaccccgg gggagcttac
      541 ggccggatgg atttcaccac ccgcgaccgc taccgccatg tcatcgagaa gatcgcgaag
      601 cggagccggt tgcacgaagc cgatgtggcg cgcgaggcga tccggatggc cgttgatggc
      661 gcggcaagga acgggccgga cgatcgctcg gcgcatgtcg ggttttacct gatcgacaag
      721 ggatcgctgc ggttggaacg ggcggccggg gtgcgcctgc cccttctcga gaccctcctg
      781 cggacggcag gccggtgtcc cctgctcctt tatctgggct cgatcacgtt gttggcggcc
      841 atcttttccg gggccttgct ggcgaaggcg catgccggcg gtttgcggga ttggccgctc
      901 gccctgttcg gcgccctctc cctgctgtgc gtcagcccgc tggcggtcgc gctggtgaac
      961 tggttcgcca cgttgctcgt gaaaccggtt ccgctgccgc gaatggattt ctccgaagga
     1021 attccggcgg aatcgcgcac cctggtggtg gtcccgacga tgctcacgag ccctcgcggc
     1081 atcgaatccc tgctcgagtc cctggaagtc cgattcctcg ccaatcggga tgagaacctg
     1141 tatttcggcc tgctgacgga tttccaggat gcgaacgagg aatcggtccc ggaggacggg
     1201 gagcttgtgc gactggcaag gggggggatc gaagagctga acgagaagta cgggggcgca
     1261 aagggagacc cgttcttcct ctttcaccgg ccgcgccgat ggaatccgca ggagcggacc
     1321 tggatgggcc acgaacgcaa gcgggggaaa attgcggagt tgaacctgtt tcttcgcgga
     1381 ggggcacggg atcgcttttc catcgtcgtc ggcaatacgg cgaatgtatc cggcgtcaga
     1441 ttcgtgatcc ccctcgacac ggatacgcaa ctgccgcgcg actccgcgcg gcagttcgtt
     1501 ggagcgatgt cgcacccgct gaaccgagcg cggtacgacg agcggaagca gctggtcgcc
     1561 gaggggcacg gcatattgca gccgcgcgtg gccgacagcc tgtccggcac gaaccggtcg
     1621 cgatatgcgc gtctgttcgg gagcgaaccg ggtatcgacc cgtacacgcg cgccgtctcc
     1681 gatgtgtacc aggacgtgtt cgacgaaggc tccttcatcg gcaaggggat ctacgacgtc
     1741 gatgcgttcg agcaggccct caagggacgg ctgcccgaga accggattct cagccacgat
     1801 ctcctggaag ggtgctacgc gcgggcagga ctgttgagcg atgcgcatct ttacgaggaa
     1861 tatccgtcca gctacatcgc cgacgtgaac cgtcgccacc gatggatccg tggggattgg
     1921 cagattgccg ggtggctgct gtcgcgcgtt cccggacccg gcggcacccg ccgcaggaac
     1981 ccgctctccg ggctgtccca atggaagatc ttcgacaatc ttcggcgcag cctcgaacct
     2041 tccgcattga cactcctgtt gctgctgggc tggaactcct tctcctcccc ctgggtttgg
     2101 accttcccgg tgctcgggat cctcctggtt ccgtccgtat tggcctccgc cctggaaatg
     2161 ctccggaagc ccgccgatac gctgctgcgc cagcaccttg ccgccgaagc gcgctccgcc
     2221 gggcggcgct tcgcccaggt ggcggttacg ctcgcttgcc tgccgtacga ggcctatttc
     2281 ggactggatg cggtcgtacg cacggcctgg cggatgctgt tcacgcacag gcggcttctc
     2341 gaatggcgac cgtcggggac cccggacccc gatcgcggcg agggcctggc ggccttttgt
     2401 cgaacaatgt ggatcgcccc cgtgatcgcc gtcgccgcgg cgatccacct cggggtttcg
     2461 aggccggtcg cgctggcgac ggccgggccc atcctggccc tgtggttcgc ttcccccgcc
     2521 atcacctggt ggatcagccg cccgctcgct cgccgcaggg cggccctgtc ggc
//
LOCUS       DATIFQ010000501        51500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_555587, whole genome shotgun sequence.
ACCESSION   DATIFQ010000501 DATIFQ010000000
VERSION     DATIFQ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 51500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51500
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555587"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VLE22_25105"
     CDS             <1..206
                     /locus_tag="VLE22_25105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17751.1"
                     /translation="AMNAFPLFLLKDEPGSVGKCVLRLFASTSHDELGDVDALAVRGH
                     FDQLLVRCGSAELEPAIARSFGR"
     gene            345..1355
                     /locus_tag="VLE22_25110"
     CDS             345..1355
                     /locus_tag="VLE22_25110"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSB17752.1"
                     /translation="MVRKLTRIVALVALCSLFVAADDSGKRVFSVMTRNVDNGTDLLY
                     VLGGVPGAISLTFDEVVASNIPARSARLATEIAAERPDLISLQEISVWTKLSAAGQPV
                     LVLDLLEELMAALEARRLRYDVVAVNNLANITQPLETGDGYVNLLDRDVVLARPELQI
                     SNVSTNLYAVFLPFGPFPVYRGWISAEVNDGRKKFRFVNTHLESLLTSIQVAQTGELI
                     QVLSQDDLPLVLAGDFNSDASGNPAALDKPDSVLLLQQAGFTEAWQALHANDPGYTWP
                     LFLEDHFPAPPFTSPAAPFERIDLVFVRGPVEIRAVERTGLVVPYGSDHAGVVATLKL
                     EP"
     gene            1411..2184
                     /locus_tag="VLE22_25115"
     CDS             1411..2184
                     /locus_tag="VLE22_25115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17753.1"
                     /translation="MKNLVCLAGALLCGASLHAQTQTPPAPQQEVRRKAYVRRLSLGA
                     TLSVLGLRLVPDRTTSVVTSSPAFDAIYTTTGASKRVGYGLNLQAAVTDRFAVNASLF
                     LRKAGYKMNSDIIEGTDDPNTSADERTLTVRNEDTRVKFYDLPVLVRYYGKDRHQRGP
                     RWFVEGGGVLRRVSNIRTSIDTTIGTGDKQCCDTTPRTPERRTLRGFVAGAGVQLIDP
                     VGIRVVPEVRYTRWAGHTFNAFSTITERNQIEGMISLTF"
     gene            complement(2215..4623)
                     /locus_tag="VLE22_25120"
     CDS             complement(2215..4623)
                     /locus_tag="VLE22_25120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17754.1"
                     /translation="MARIASRILGIGVVITAVGLLVARQTVRPSDPSRSPGHSNAALA
                     SITIDYPEEGSIFPPEIIPPTFLWRDATESARLWRVDVTFPDGSAAIRAMSTGERLQI
                     GEIDPRCIANTNELPKLTPQQAAARTWTPDAETWAAIKTRSTTHPATVAITGLRGTNP
                     DQPVSRGQVSIRTSKDPVGAPVFYRDVPLMPSELEKGVIKPLPPSAIPLVAWRLRNIA
                     EPRSRLLLDGLPTCANCHSFSLDGKTLGLDLDGPRNDKGMYAIASISPQMSIHNEDVI
                     SWDSFRDRPASPMRVGFMSQVSPDGRFVVTSLNGEKTDLAGSYYVANFKDYRFLQVFY
                     PTRGILAWYSRDSRRIQSLPGADSPRYVQTDAVWSPDGKYIVFARARAREPYPVGRKM
                     AEFAGDPNETPIQYDLYRIPFNEGKGGEATPIAGASGNGTSNCFPKVSPNGRWLVFVQ
                     CRNGQLMRPDSKLYIVPVEGGEARRMRCNTPLMNSWHSFSPNGRWLVFSSKARSPYTQ
                     MYLTHLDEDGRDSPPILVDNATAANRAVNIPEFVNIPPEGLLKIDVPAVEFYRLYERA
                     WELTEKGQDDAAIAAWKQALVMSPGDGRAHSNLGAVLLRRGKLDEAISHFERALEVNP
                     GSPASYNNLGALLLRKERFNEATSQFQKALKINPEFAEARFNLASAFYMQGKTFDAVA
                     QWREGLRLEPTRVSVLTQAAWALATSPKASVRNGAEAVVFAEQAVRLSGGRDPEILDT
                     LAAAYAEAGRYSAATQTARRALALAEKSNNGSLADAIKGRIVLYEARKRFRETKPLSP
                     SPPR"
     gene            complement(4717..5730)
                     /locus_tag="VLE22_25125"
     CDS             complement(4717..5730)
                     /locus_tag="VLE22_25125"
                     /inference="COORDINATES: protein motif:HMM:NF014753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HSB17755.1"
                     /translation="MKKLINDPNRVREELFEGFLAAYGKQYNLIRVGEGTVAVRRDIP
                     DGRVAVLTGGGSGHEPLFLGALGPGMADAAVCGDIFSAPSPDLIVAGAGAVHRGAGVC
                     MIHGNYAGDNMNFRIACEMLAEEGIPTFQLRVWDDIASAPPARKEERRGMTADVFLIK
                     IAGALAERGASLAGMVPVIERAREYCRTLGVALTSATVPQTGKPTFEIGESEMEIGIG
                     MHGEPGVGREPMASADSTCRRMIDRMVADLPFRAGDEVLLLVNSMGATTYMELYIMAR
                     AAHRVLAEYGISVHATHAGPFFTCQEMAGCMITLMKLDEELKSLYHAPAHSPDYFLMR
                     ALE"
     gene            complement(5727..6368)
                     /gene="dhaL"
                     /locus_tag="VLE22_25130"
     CDS             complement(5727..6368)
                     /gene="dhaL"
                     /locus_tag="VLE22_25130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HSB17756.1"
                     /translation="MDAPALIRALALMGERLREQKDWLSGLDAACGDGDHGVSVARGF
                     EAVEENILAWAQLAPGPILRQCGRVLLSSTGGAIGPILATFFSELGKACGDSGEIDTP
                     TMAVMLQASLDGVTRLGGARPGDKTLLDALAPAVDSLRRDADDAVPLRRAMHRAAEAA
                     AGGARETAGLVARQGRARYLGERSIGHPDPGAHTMAIILTAVADAIREEEPRA"
     gene            complement(6454..7473)
                     /locus_tag="VLE22_25135"
     CDS             complement(6454..7473)
                     /locus_tag="VLE22_25135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17757.1"
                     /translation="MRYQAPWFVLAALCLPPSPAIAQHSGDSVREVFYPTVAMSNSFT
                     QPMVVVDRGFLIAIRNRNYLIVHDRDGKSIHDIELKLPGTETVSISDVAMKRDGTAVL
                     ALIHTPAERGYGGAIAILDPAGKIRRFIQTGLYMPLQICLDEEDRIWTAGWQRDADRI
                     LEEDKADYPLIRKYGLDGTLILGAVKRTSVDKGFVSGGHGRWKMRAAHGLIGILSFRI
                     DDKPEVWTELNLKGEIVGQWTLGPVPLGRKIDGAEFDDHYSRFKYSLGFTVNGELYAL
                     CWSCSTDDKHFRLARLDRKSGDWKIMRETPRDNGILIGSDGNELVLARGFGGVQLTWI
                     KPTMN"
     gene            complement(7567..9441)
                     /locus_tag="VLE22_25140"
     CDS             complement(7567..9441)
                     /locus_tag="VLE22_25140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSB17758.1"
                     /translation="MTGRLAFLLVIAALAAAQSTPQYDIVTAKKVMVPMRDGARLSTD
                     IYFPARNGVPVPGKFPVILERTPYGSHTIESWASYFVPRGYIAIGQNVRGRYGSEGEW
                     FPHRDDVRDGYDTAEWVGKQPWFSGKIGTVGTSYPGGTQHALAMGNPPYLAAMVPVDS
                     MSNPGLYGVRHHGAFELRFFNWIFSLGNSPSGGYGPTVPQHYPTSDPATRAALADLRN
                     HVIEYIKALPLRPGTTPLRFAPEYESWLIEAMSHGDYDEFWKNCGSDVVSHIAEYKDI
                     PVYHVSGWYDSWGLQVANLNYVELARAKKSPQRLIMGPWTHGGQTASFAGEAEFGPDA
                     AIDFNALRLRWFDWWLKGSDNGVDRDTPVRIFVMGGGDGHKTTEGRVFVGGQWRDERE
                     WPLARAKPTSFYLHTGGRLAAAPPVQAPPTRYLFDPRDPVPTIGGNVSSEGLLMPRGA
                     ADQKCRKELWSCKDTLPLSARNDVLVFKTDPLESPMEVTGRLIVNLWVSSNAPDTDFT
                     AKLVDVYPPNRDFPAGVDLNVADGIIRARYRESVELSKLMQPGEITKVTIEMYPTSLV
                     FGKGHRIRLDISSSNFPRFDLNPNTGEPLNNNRRWAIAENSVYHDPQHASHIVLPVIP
                     "
     gene            9537..10535
                     /locus_tag="VLE22_25145"
     CDS             9537..10535
                     /locus_tag="VLE22_25145"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HSB17759.1"
                     /translation="MKEPERGKEILYLTRQEVIDIGLTKKDILELVRAALTEHGNKRC
                     EMPAKIGIHPLRDTLMHAMPAWVPARQACGIKWAECFPDNYEKYGLPQTSGLMVLNSP
                     ETGWPICILDAIWVTAKRTPAVTALACESLARKDTAVVGIAGAGVQGREHVLVLPEVL
                     PKLKKIKLYDKFPEVTRKLIADLQPEVPGVELVEVPGFEELVRGSLVVVTGTAILMAP
                     DPQIRDEWVEPGAFLAPVDFDSLWEWATFSRADKFLVDSLDEMKYFMTVGYLPHGLPP
                     LHAEIGEVVAGLKPGRERDDELIIDMNIGMGVEDVVVGREIYRRAVASGAGTILPL"
     gene            complement(10548..11744)
                     /locus_tag="VLE22_25150"
     CDS             complement(10548..11744)
                     /locus_tag="VLE22_25150"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HSB17760.1"
                     /translation="MKQISLTAWIFIGMAVGIAFGLFAPGFALNLAPVSNIFLRLIKS
                     IIAPLLFGTLVYGIAGTGNIRAMGRIGLKALVYFEIVTTAALFLGLAAVNLVQPGAGM
                     KLERTASESTVAVQPPTLGNFLEHVFPASVFDAMARGEVLQLVVFAFLFGTACAAIGA
                     KAKPVVDFAESLAEVMFKYTGYVMWLAPAGVCAAMAVTVSSKGLDVLFGLGKLILTMY
                     ATQAIFVVVVLGAVMAIARVPVGRFYRAAREPFLIAFSTASSEAALPRALENMERFGV
                     PKHIVSFVIPTGYSFNLDGSTLYLSLASVFVAQAAGIELTLGQQLLMMLTLMLTSKGV
                     AGVPRAALVILAGTLATFQLPIEGAAVLLGIDAVMDMARTSVNVLGNCLATAVIARWE
                     RVPVES"
     gene            complement(11741..12604)
                     /locus_tag="VLE22_25155"
     CDS             complement(11741..12604)
                     /locus_tag="VLE22_25155"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HSB17761.1"
                     /translation="MPGMKTIGIISKPNVRQASAIVPALIEWLASRDLHVRCDEFTAA
                     YAGRTDGFSRAETPEGCDLVVVLGGDGTLLAAARAVSAREIPLFAVNLGGLGFLTAIS
                     TDELYPELERALRGEHRIGKRRMLHCDLVRGERTVSSYESLNDVVVTKASIARMIDLE
                     AFVDGHFVCHYKADGLVISTPTGSTAYSLSAGGPIIFPSVGALCITPICPHMLTNRPV
                     IVPESSVIRVWNHGDDNETYLTVDGQVGEPLMRDDRVICSSSSHYIHLIRPPRMLFFD
                     VLREKLRWGER"
     gene            complement(12570..13325)
                     /locus_tag="VLE22_25160"
     CDS             complement(12570..13325)
                     /locus_tag="VLE22_25160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HSB17762.1"
                     /translation="MKLVFRQRLDQLLVERGLAESREKAKALVLAGHVLIDGQKAGKP
                     GQTVSTQCRLELVERLPYVSRAGIKLAAALDRFGIDVTGRVCLDIGASTGGFTDCLLQ
                     RGALRVYAVDVGSGQLDWRLRNDTRVIVREGINARYLRFEEIGERAGLAVCDVSFISV
                     TLILPAVVPILQAEAEMVILVKPQFEVRRGEVGKGGIVRDPELHQAACARVEGAARAL
                     GFRTTLMNSPILGAEGNKEFLLYARHEDDRNHL"
     gene            complement(13322..14206)
                     /locus_tag="VLE22_25165"
     CDS             complement(13322..14206)
                     /locus_tag="VLE22_25165"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HSB17763.1"
                     /translation="MILSEYLTEQIRLVDAELDRWTPAESTPPSIIHRAMRYSLFAGG
                     KRIRPVLCMEAARAVSGQCEGVEAASCAIEMVHTYSLIHDDLPALDNDDLRRGKPTCH
                     KVFGEAMAILAGDALLTCAFEVLASVSTVPLDRRARMVRELASAAGTPRGMVAGQVAD
                     LEGEHQAPEPKLLETIHRAKTGALIRASLRIGAIYAGADDMQFEALSCYGEHVGLAFQ
                     IVDDILDVEQSSEALGKTAGKDAQQHKITFPAVYGLKESHRMAEEERLSAHRVLERFG
                     DRAARLRELADLIVHRKA"
     gene            complement(14203..14457)
                     /locus_tag="VLE22_25170"
     CDS             complement(14203..14457)
                     /locus_tag="VLE22_25170"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HSB17764.1"
                     /translation="MAKTPEPEKQPLASFEASIEDLEKVVKELEAGDLPLERALALFE
                     KGVGLSETCRKQLEEAESRVEILLKRNDKVQPEPFHPENE"
     gene            complement(14723..14950)
                     /locus_tag="VLE22_25175"
     CDS             complement(14723..14950)
                     /locus_tag="VLE22_25175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSB17765.1"
                     /translation="MEQVRRVTAVIEREHDVYVALCPELDIASQGNTIEEARTNLVEA
                     LTLFFETADPSEVEHRLRQEVLVTQVEVRVA"
     gene            complement(15015..15398)
                     /locus_tag="VLE22_25180"
     CDS             complement(15015..15398)
                     /locus_tag="VLE22_25180"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSB17766.1"
                     /translation="MRRLISSGSTFEAEIGFSRAVVDGDWVFVSGTTGFDYRAMTISD
                     DLIEQTEQCFRNIEQALAEAGCSLRDVVRMRYILPKAEDFPLCWPVLRKYLGDIRPAA
                     TMFVAGLIDPRIKIEIEVTAKRAGM"
     gene            complement(15416..16501)
                     /locus_tag="VLE22_25185"
     CDS             complement(15416..16501)
                     /locus_tag="VLE22_25185"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HSB17767.1"
                     /translation="MSEETLYFPDSVLTVFSRQLLEAAGAPTPNAAVMAECLVSGSLR
                     GVDSHGVQLLRFYVEQIEAGGVNLTAEGHVISESGACLNYDGENGLGAVVSERCCGHA
                     VRLAREHGAGIVIARDSNHFGAAAFWGQRISSEGMVGIVMCNATPLVAPWQGREGRVG
                     TNPICVTVPGTGGRGWLLDMATTTVAMGKIYRAAMSGQPEIPPGWAMDSEGRPTTDTE
                     TAMHGGLLMPLGGYKGSGLGMMVEIFCGALGGGAMSTDVGGVRTRGCATRISQTFIAI
                     DVARFMPLEQFRARLDELVSRVKATAPAAGYDEVLVAGEPEWRAEEDRRRTGIPLNAG
                     VWEELVKAAHRLGVTPPDASHSPLRLP"
     gene            complement(16515..17477)
                     /locus_tag="VLE22_25190"
     CDS             complement(16515..17477)
                     /locus_tag="VLE22_25190"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSB17768.1"
                     /translation="MTRRAATFVLLIAVALAGCRGSRKKVIAVIPKGTAHLFWVSVQA
                     GALAAGRELDVDVLWNGPNQENEYSRQIQIVDSMIARRVDGIALAAAERTALVQAVDR
                     AAAAGIPVTIFDSGLDSENYMTFVATNNYEAGQLAARKLASLLGNRGKVAVVMHAPGS
                     YSTMERERGFDDVMAKEFPDIRVVARQFGMSDRAKSVAAAENILTAHPDLDGIFASTE
                     PSSSGASLALKARGLGGKVKFVAFDTSDAMIEDIKSGVIHATVVQDPFRIGYEAVKTL
                     VDKLHGKNPPKRIDLGGTVVSPENLQTMEIQQLLRPNINKHLQK"
     gene            complement(17474..18232)
                     /locus_tag="VLE22_25195"
     CDS             complement(17474..18232)
                     /locus_tag="VLE22_25195"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSB17769.1"
                     /translation="MSDRKLAGRVALITGASRGLGRAIATAFAAEGASIALVARDLEK
                     LNETASAVRNAGGEAEVFRADVSDEAQVLRLETDVITRFGGLHILINNAGVNLRKPIT
                     DFTLEEWRRIIDTNLVAAFLTCRAFVPHMKRGGYGRIINLTSTMSHVALPGRTPYCAS
                     KSGLLGFTKALALELAPDGITVNGISPGPFATEMNTVLMQDEEVNRQFMAKVPLGRWG
                     KVEDVGRLAVWLCSEEAGFVTGTDVLIDGGWCAQ"
     gene            complement(18235..18687)
                     /locus_tag="VLE22_25200"
     CDS             complement(18235..18687)
                     /locus_tag="VLE22_25200"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSB17770.1"
                     /translation="MEEFVIRPTMKLIRAGYTVISLIFIAAVLAYTNSAAMESVSAWV
                     LLAPALLFLWPLSRQIRRGLTKMTIQGDKIRHESGLLTKTTRNIQISKVQDVRVDQTL
                     FQRLLGVGTLAIETAGETSLLTIHDIDDPHGVADRVSRAAHLSTDKEK"
     gene            18776..19858
                     /locus_tag="VLE22_25205"
     CDS             18776..19858
                     /locus_tag="VLE22_25205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17771.1"
                     /translation="MKRTLLAVSAVLCVVAGTARADFSYEQSSKITGGFLAGMMKFAG
                     VFSKKATEPVKTSVQVKGDRMANIGSESAQVVDLGKETITDINFKNKTYAVITFAQMA
                     EAMQRMMEKAKQQQGAENVDANFKISVTPTGQTRQINGLDTKEVILKFEIESKDKKSG
                     EQGSLTTTSNMWLAPKISGYEEVTSFHQRMGQKIAWTPGGMALAQGRSDIAKMMGNVS
                     KETAKMDGIPVLQIMRMGGEAQGQPAQAGQPAEAGQPAPQAAPADQQEQGSAKAVAGA
                     LGRLGGFGGFGRRKKKAEEQPPQQQPEQQQQQAAGQPGAPAGGVLLEMTTELTGFSAA
                     PVDASKFEVPAGFKQVEHEMVKALRK"
     gene            complement(19910..20098)
                     /gene="rpsU"
                     /locus_tag="VLE22_25210"
     CDS             complement(19910..20098)
                     /gene="rpsU"
                     /locus_tag="VLE22_25210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HSB17772.1"
                     /translation="MTIQDGESLEAALRRFKRKVQQEDIIKEIKRHSYYLKPGEKKRV
                     KAALARKRNRKKRPRDFE"
     gene            20223..21461
                     /gene="glgC"
                     /locus_tag="VLE22_25215"
     CDS             20223..21461
                     /gene="glgC"
                     /locus_tag="VLE22_25215"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HSB17773.1"
                     /translation="MHKVLAILLAGGAGERLYPLTRNTAKPAVPFGGVYRIIDFTLSN
                     CINSNIRRVFILTQYKALELHRHVREGWNILSPELGEYIEVFPPMKRIHEDWYLGTAD
                     AVFQNTHSIIAEDPECTIILAGDHIYKMDYGEMLDWHRSHRADITVGTIQVLPEEAPR
                     FGIAEITPDHRIVGFEEKPSHGGPQRSVFNPEMVSASMGVYVFKTPVLLNALAQDAED
                     PASSHDFGRDVIPRWISKARVIAYDFHDLNDKTVHYWRDVGTIEAYYEANMDLVAVIP
                     QFNLYDSNWPIRTRLEQHPPAKFVFAQEGRRMGVAIDSIVAGGCVISGGRVIRSVLSP
                     GVRVNSYCEVENSILMNRVEIGRYCRIRNAIVDRDVKVPDGATIGFNADEDRARGYHV
                     ADGGIVVVPSAQETYATIAV"
     gene            21564..22862
                     /gene="eno"
                     /locus_tag="VLE22_25220"
     CDS             21564..22862
                     /gene="eno"
                     /locus_tag="VLE22_25220"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSB17774.1"
                     /translation="MMTKIVKTVGREILDSRGNPTVEADVYLADGSMGRAAVPSGAST
                     GEHEAVELRDGDKSRYLGKGTKNAAQNVNGEIAVAIQGMDAARQEEVDRKMIALDGTP
                     NKGRLGANALLAVSMAAARAAAASQQVPLYRYLGGVSASILPVPMMNILNGGAHADNS
                     VDAQEFMVSPVGAPTFAEALRMGTEIFHSLKAVLKKKGYSTAVGDEGGFAPNLKSNDE
                     ALEVILEAIEKAGYKPGVDVCLALDPAASEFYDTGKGKYIFKKSDKSEKSPEQMVEFW
                     ANWVRQYPIISIEDGMAEDDWKGWKLLTDTLGPKIQLVGDDLFVTNTLRLARGIQEGI
                     ANSILIKVNQIGTLTETLEAMRMAAAAGYTSMVSHRSGETEDTFIADLVVATGAGQIK
                     TGSASRTDRIAKYNQLLRIEEELGASAKYAGLKAFKRLAI"
     gene            22900..23649
                     /gene="gpmA"
                     /locus_tag="VLE22_25225"
     CDS             22900..23649
                     /gene="gpmA"
                     /locus_tag="VLE22_25225"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HSB17775.1"
                     /translation="MYKLVLVRHGESTWNKENRFTGWTDVDLSEKGVGEAKDGGRVLK
                     EQGYTFDIAYTSVLKRAIRTLWLVLDEMDLMWIPVVRDWRLNERHYGALQGLNKSETA
                     EKFGEDQVKIWRRSYDIPPPSLEKSDPRWPGHDARYKSLSEEQLPLTECLKDTVQRFL
                     PMWHDVIAPIVRSGKKVLIAAHGNSLRALVKYLDNVSDQDIVGLNIPTGMPLVYELDA
                     DLKPIRSYYLGDPEKVKAAMEAVASQGKAKK"
     gene            complement(23678..24205)
                     /locus_tag="VLE22_25230"
     CDS             complement(23678..24205)
                     /locus_tag="VLE22_25230"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSB17776.1"
                     /translation="MLTVSTLARACGLSRSTVLYYESIGVLKPAGRTGGNCRRYGDRE
                     MARLRQICVYRKAGVSLANIRNIFDRPNSDASSILKRRLTELDAEIEQLRDHQRAILR
                     ILNRHSFRRSDVLNKEKWVSIMKASGFTEADVDRWHKEFEKMAPEEHQKFLEFLHIPA
                     DELAAIREWSRTGKK"
     gene            complement(24426..25508)
                     /locus_tag="VLE22_25235"
     CDS             complement(24426..25508)
                     /locus_tag="VLE22_25235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB17777.1"
                     /translation="MTTIRCVCLGLLAVALLAQQPETSDQVFRATVNVVSAPVTVTDH
                     EGRYVHGLQPENFRLLDNAKPQDIKVDVSFVPISLVVAIQTSWATQAVLPKIQKIAPL
                     LQGLVVGEQGEVAIISFAHRVDVVQDFTSDSDNISKAINSLKIGASSNRMIDATQTAI
                     RMLARRPPDHRRVLLLISETRDNGSEGRARDALTSAQLANVLIYTVNINRMMTTFTAK
                     TQPPRPDPVPPSARPLPAGVPPTPEAARQVSGLEGSSASFLPLFVEIFRQAKSVFVDN
                     PAEVFTEFTGGREYAFTRERDLENAISRIGEELHSQYLVSYMPNNKMEAGFHEIQVQI
                     FGSSGNVRRDLKIQTRPGYWLAAVPD"
     gene            complement(25527..26036)
                     /locus_tag="VLE22_25240"
     CDS             complement(25527..26036)
                     /locus_tag="VLE22_25240"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HSB17778.1"
                     /translation="MIRVAVLTISDSAHAGSRSDLSGPAVKRRLKEAGWQVAVLEVLP
                     DEAEIIAARLAALTDGGRISAIFTTGGTGVAARDVTPEATRSVIEKEIPGLGEVMRAH
                     GRNSTPLAVLSRGIAGTRGKTLIVNLPGSPRGAVESLDAIVELVPHILELLRGETEHA
                     VPVKPKYGP"
     gene            complement(26033..26515)
                     /gene="moaC"
                     /locus_tag="VLE22_25245"
     CDS             complement(26033..26515)
                     /gene="moaC"
                     /locus_tag="VLE22_25245"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HSB17779.1"
                     /translation="MKKLSHYDDAGAARMVDVSDKPDTKRTASAQAFVRIRRAVLRKL
                     PENPKGNPLEIARIAGIAAAKRTSELIPLCHPLMVSHADVDVRVERDGVRIVATASTT
                     GPTGVEMEALTAASVAALTVYDMTKALDKSIEIQDVYLIEKTGGKSGDFRRPARGRKA
                     "
     gene            complement(26512..27705)
                     /gene="glp"
                     /locus_tag="VLE22_25250"
     CDS             complement(26512..27705)
                     /gene="glp"
                     /locus_tag="VLE22_25250"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HSB17780.1"
                     /translation="MPTLSFREARECVRSMITAARPQPRVEQARLLDAADRVLAEDLV
                     ADRDYPPLARSIRDGFAVWHADLPGRLRIVGEVRAGEVYKGVVGPGEAVEIMTGAPLP
                     EGADTVVMVEHVRRDGGFVVVEGDTKPGQFVNPRASEARRGARLLEPGRRLDFSTIAL
                     AATIGKTAIKVYAKPRVAIVATGDEVVPVGEEALDHQVRNSNAFSLAVQVSVAGGTPE
                     ILPVARDEHEATRKLVERGLGADLLLLSGGVSAGKYDIVERVLAGLGAEFHFDRVLIQ
                     PGQPLVFGRARGTFFFGLPGNPASTMVTFRLFASAAVELLGGQSGVALPIFQARLTKD
                     FRHKTGLTRFLPALLSPDGAEVMPLPWQGSSDVPALARANVFLVADPEREHWAAGELI
                     GVLLK"
     gene            27791..29254
                     /locus_tag="VLE22_25255"
     CDS             27791..29254
                     /locus_tag="VLE22_25255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB17781.1"
                     /translation="MRAETSNFIRRFSRVCLSAGIAGGIAFLDSRFAHVNSTTVALML
                     VLVVLAVATRWGLPESMGASVAGMLCLNYFFLPPVGKWTIADPQNWVALCVFVITAVV
                     ASQLSASAKRRTAEANSRRREMERLYVLSRSLMRMDTESPFARQIVSRIAEVFALDRV
                     VFYDRDDDAVYGPGSVDVASMEGPLKESVVGGTSTVINECGATVSPVNLGSVVIGSLA
                     LPTESMSGSARRAITQLAAIALDRARGQEAASRAEAARLSEELKSTMLDALAHELKTP
                     LTSIKAAVTAVLGCNSSVPTQTELLQVIDEETDRVTSMVTEAIQVARIEAGQIELRKA
                     PHSMAALVRQVLSLFSGCLESRTVEVQVSESLPPVEADAELVRIVIRQLVDNAAKYSP
                     PASPISIRAMQRGESIVLSVADRGPGISGPDRERIFERFYRGHAGRGPVSGTGMGLAI
                     AREIIRAHNGEIGVESQLGRGSEFHFSLPVARAEAEP"
     gene            29251..29946
                     /locus_tag="VLE22_25260"
     CDS             29251..29946
                     /locus_tag="VLE22_25260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB17782.1"
                     /translation="MSARILVVDDEPQIRRVMRATLTAQGYTVSDARSGEEALEKLGQ
                     SPQDLVLLDVNMPGLGGLAACRAIRAGFDVSIIMLTVRNAEKDKVEALDAGADDYVTK
                     PFGTPELLARIRAALRRSASIAPAGAQPMTIGEFMIDFQGRQVVIGERHTRLTPKEFD
                     LLWHFVTHANRTITHRDLLQAVWGLEYGGEVEYLRVFVNQLRKKIEPDPANPKYLLTK
                     PWVGYRFQLPGQR"
     regulatory      30091..30222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            30255..32474
                     /locus_tag="VLE22_25265"
     CDS             30255..32474
                     /locus_tag="VLE22_25265"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HSB17783.1"
                     /translation="MTIQNAPVTTKVVVATSVMLSFISFWRAAAIVLNDLASTAYYVG
                     GIAETAIGKAAPWFILAIMLFSYAVRAIYIESCSMFVRGGVYRVVQEAMGGTLAKFSV
                     SALMFDYVLTGPISAVSAGLYLGGLINETAESFHLANVQLHPPHFAVGFAIAVTLYFW
                     RKNIIGIPESSQKALRIMQVTTVMVVILIAWCLITIAKKGYQPVPAPVVSNLHFSDGA
                     LGWLKGTVLPAIPMIAILIGLGHSLLAMSGEESLAQVYREIAHPKLKNLERAGFVIFL
                     YSLLFTSLVSFFAVMIIPDGERGKYLDNLIGGLSVFLVGPAPLRLLFHGFVVLAGILV
                     LSGAVNTSIIGSNGVLNRVSEDGVLPDWFRHPHRRFGTTHRLINLVVILQIITILISR
                     GDVYILGEAYAFGVMWSFSMKALSVLVLRYKRPEGREWKVPLNFRVGTTEIPVGLGLI
                     TLGLVSLAVVNVLTKKTATLSGLAFTAAFFVAFELSERYYRGTERRHATQLEKFRVEA
                     SEDLSAIAVGARPGNVVVAVRNPGHLDHLKKVLDKTDTRKIDIVVLSVRVISPAGSGE
                     HALSPGQIFADDETRLFSKVVSLAEHAGKPVRLLAAPATDPNLALVQVAETLQATRIV
                     AGPSARMGLDEQARLIGLAWERLPEPKSPVSYEIVSLDGRSQFYDLGPHLPRLWPEDI
                     EVAHRLWLDLTDSLGAQLHHRDVVGLALRRMESDLKSGRAAEVAEALSREIGSRPQG"
     gene            32552..33958
                     /locus_tag="VLE22_25270"
     CDS             32552..33958
                     /locus_tag="VLE22_25270"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HSB17784.1"
                     /translation="MPHRMTMVQMAAEVRAGKVSPSELVQAHLAQIEKHNPTLNAFAI
                     LRAEEVREAAGTAEQALGQKNLGPLHGVPVTVKDSFDLAGLPTLCGSRFRLGHKAASD
                     STVVSRLGKAGAIVLGKTNTPEFLMSYETDNHLTGRTNNPWDLERTPGGSSGGEAAAI
                     AAFCSAGGVGSDGGGSVRIPAHFCGICALKPTPGRIPATGHFPDICHPGGLLGVAGPM
                     ARTAQDLRLLFTVLSGHDAQDPFSAPVPIGTPEFKELRAGVLEQFGADPVQADVRQAV
                     WRATETLGELGIPAASFEPRGLDRAQELWWFFFGRLNAMLIRQVVREREDEAHWTGME
                     LVTMAEQEPPPGAMEILENLAARDRMRAALLRQMEETPILLLPACGVTAFRHREREYE
                     TPDGPIGLLEAMAPATPWNLLGFPAVVIPFGMTYDGSLPVGIQIVGRPWEEELILEVA
                     IRLEEARGPFLAPPGYRD"
     gene            33974..35308
                     /locus_tag="VLE22_25275"
     CDS             33974..35308
                     /locus_tag="VLE22_25275"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSB17785.1"
                     /translation="MLRVIFLLLLGVLVAAAAGSPKKAFPFDYDQHDFPNGLRLITVP
                     TDYPNVVALYIVVQTGSRNEVEPGKSGFAHLFEHVMFRGTKEYPPERYEAVLKAAGAA
                     SNAYTTDDHTAYHTTFSKEDFESILKMEADRFRNLSYSEGVFKTESLAVLGEYNKNSA
                     APTSKLEEVLRDTAFDRHTYKHTTMGFLKDIQDMPNMYQYSLQFFDRYYRPEYTTIIV
                     AGDVKKDSVPSLVEQYWGGWKRGSYTAAVPKEPPQDAPRSNHVAWNTPTLPWLTVAFR
                     GAAYSDTEKDQATLDLIAFLGFSESSELYQKLVIQEQKVDVIEPDNSDHVDPYLFSVW
                     SRVKNGGDVNYVRDQILATLESFKARPVPKDRLEAVKSHLRYSFALAMDNSESIAATL
                     ARYVALRRTPETINKLYEMYARITPEDVQDIASKYFSENSRTVVTLAGGKAQ"
     gene            35305..36780
                     /locus_tag="VLE22_25280"
     CDS             35305..36780
                     /locus_tag="VLE22_25280"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSB17786.1"
                     /translation="MRTLLIPMLMLAATGLSAQMRVVAQRSDSPLVTFRIVFLTGAAA
                     DPSGQEGVASLAASMLSQGGTREMSYKQIVDAMYPMATSVSSQVDKEMTVFSATTHVD
                     NLDAFYKIFRAMLLDPGWRADDFERLRDDAINFLRVSLRGNNDEELGKEVLYNVIYNG
                     HAYGHHNAGVVSALQKMTVADLQTFYKANYTKDNLILGIAGGYSPAFLERVKKDFEEL
                     PAKGKHTPKYDAPKAVRGIRVTMIEKDTRSVAYSFGFPIEVKRGHPHFPALLLAQAYF
                     GQHRMSGGRLYNRLREARGLNYGDYAYIEYFPRGMFQFEPDPNLARPSQIFQIWIRPL
                     EPATAHFALRLAMYELDKFVREGISKDDFERWRSFLSKYVNLLTKTKAAELGYAIDSQ
                     YYGTPEYTTYIRNSLAKLSVDDVNRAIRKHLRTTDLDLVIVAKGCEELKKSFLGGAPS
                     PMNYNSPKSAEILEEDKIVEKWKIGFQPEAFRIIPVDQVFE"
     gene            complement(36784..38445)
                     /locus_tag="VLE22_25285"
     CDS             complement(36784..38445)
                     /locus_tag="VLE22_25285"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB17787.1"
                     /translation="MRRTTLLIPYVAALCAISISALFAQSAAPAPVKFRNVAVSAGIN
                     FVLENHPTPKKHLIETMAGGLAAFDYDGDGLTDIFFTNGASIPSLEKDSPKYRNRLYR
                     NLGNMKFKDVTDQAGVAGAGYSIGAAAADYDNDGHTDLFVGGVRRHFLYRNRGDGTFE
                     DVTRKAGIKSEWWSEGAAWLDYDNDGKLDLFVVNYLQWTASFDLFCGDPASKVRAYCH
                     PRFFEGTPNTLYRNRGDGTFEDVSQKSGIAAHIGKGMSASIADYDRDGFMDVFVTNDK
                     IPNFLFHNKGDGTFEEVALLAGGALSDNGAPVSAMGTDFRDYDNDGFPDITYAALAGE
                     VFPIFQNRGNGTFRDASFASRMGPLSHNRSGWSIGLVDLNNDGWKDIFTTNSHVNDTV
                     EMFEATPYKLPNSFFLNRGDGTFVDGSRDAGPDFQTPAAHRGCGFADFNNDGRIDVVV
                     ASLQSPAELWENVSSADNSWLILKLTGTKSNRDSIGAVVRIGKQFNHMTSAVGYVSSS
                     HFGVHFGTGQMKRIDEIEIIWPSGVHQTVKDVGVNQVLKVVESDR"
     gene            complement(38576..39619)
                     /locus_tag="VLE22_25290"
     CDS             complement(38576..39619)
                     /locus_tag="VLE22_25290"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17788.1"
                     /translation="MPPLRVMPFVLVLLPMSGFAQVTGLAVCDLLRSAELSRTGREAL
                     ASKQYPTAAHDLREAYKACPAQHGLLLELSRALSYQREHQEAIRVAREYIQSEPRSAE
                     GHVALASALFMAQELTEAKQEAEEALTIEPEQPDAFKLRGNCEYLLGETEKAQKTLAE
                     LLARHPNDEDAAYMLGRIYYQEGRIDHAIGQFQRVLKINPNSYKAYDNLGLCYEAKSE
                     KEMAIRHFLTAIKMVETDHPEYDWPYANLANLLIDNGDAKQAFSAAVKAAQRNANSAR
                     NFYLAGKALWKLEQYETSVKWLERSTFLDPDYPEPLYLLARVYSQLGQKDKASETIQR
                     FRDVKSKTPRQRR"
     gene            complement(39637..41667)
                     /locus_tag="VLE22_25295"
     CDS             complement(39637..41667)
                     /locus_tag="VLE22_25295"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB17789.1"
                     /translation="MLSAKFSVRVLCVFAALLLVSCRSTAKKPASGGEANAPLRKLNV
                     VVVTIDTLRADRLGCYGYSKIETPVLDGLAKRGALFENAVAETPLTPPSHASIFTGQN
                     PNVHGVRNTGGFVLQSSSTTLAEILQQQGWDTAAFIGASVLKKLFGFNQGFAVYDDEM
                     PKPGKGQDAREYPERPAGAVVDRAIQWLNGQSGKPYFMWVHVFDPHMPYEPPAPFRQQ
                     YAGRPYDGEVAYADHELGRLFEAVQKKDNAGNTLIVVLSDHGESLGEHGEYTHGIFLY
                     DVTLRVPFILAGPGVPGGMRVTRQARTIDVLPTILDLMGGKPPGGIQGTSLVPALAGK
                     DVATQHAYAETLYPKMNMGWSELRAVRTLRWKFIRAPKPELYDLSQDPHETANVIDRF
                     PTERAEMEAQLKAIAGDGKEKVDTAMVDQRTLDQLKSLGYLASFTPRQFELTGTGIDP
                     KDRVNILRIFELAVSGESKLPPAKRMEMLKQAVAEDPTNPSVYYHLGTEYEKAGRFKD
                     AMQLYQSATEKGIRNGRLYSRIADLYLREGNKSEAIFNYEKAAQFNPSDVESQNNLAM
                     AYLENGRVADADRVYNWILATGEQHAPAYNGLGLIAIQRQDPATARGYFEKAVAIDPN
                     LVEAHLNLGLIYKMMGDRARARACFETFLAKASSRQYAGIIPKVKEELVLLR"
     gene            complement(41713..42399)
                     /locus_tag="VLE22_25300"
     CDS             complement(41713..42399)
                     /locus_tag="VLE22_25300"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HSB17790.1"
                     /translation="MEHKGLRAANQTDAELFERIESTLYTAAVADALDELGIRNHAMT
                     ENLRPVTVRHCFAGWARTIACVDQHYTPAEPYAKEIEAMDSILPGEVVVVSTGESRRN
                     APWGELLSTAAVARGARGAVIHGLVRDVKKIDEVGFAVFAAGIKPVDSKGRGIVVEYA
                     TPVECGGVVVSPGDLVFADFDGVVVVPAAVVPDAIRLATEKVTKENHTREELRRGAYL
                     RDVYEKYGVL"
     gene            complement(42558..46073)
                     /locus_tag="VLE22_25305"
     CDS             complement(42558..46073)
                     /locus_tag="VLE22_25305"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB17791.1"
                     /translation="MHKSYLKLVLCLLASLSVAWGQGSVGTLTGTVLDASGAVVPGAT
                     VVATNVATAVENTATSTSAGAYTLPYLPAGTYTIKAGAPGFRTSTREGVILRVGQTLS
                     VNINLELGAVTEQVTVSAAPPLLEAGSAEIGRYISTEEYKSWPIFVDDGQRQIQQFIF
                     DALPGTTGGTFLGSINGGQQYSHEILIEGMAVGRSDLSGGNNNEFSPSAEAISEFKLQ
                     TGAIGAQYNGGQTAVANFSIKSGTNDLHGSAFYYGQNEYFNAFNLSQKTQGITKKSRH
                     REHNYGYSVGGPVYIPKIYDGRNKTFFFTNFEKDTRNELGFNGFTTLPTTDQKQGDFS
                     RFLDPTFTGNADSGTTIGTDALGRPVVFGQIYDPLSTRTVNGEIVRDPFLDNVIPGNR
                     ISPVARNVLDKVGIADPDFDKMIRNKNRLSTSSPYFNLHLIGVKGDHVINDKHRVSGF
                     YNQSYRKRNNNGSSRYLPIPGTATSSWQEQITPGKMVRASLDSTVHPTILNRFAFGFN
                     SFRNQNGAIPDTYQAGWPALLGISGIPDTIFPTFSFSGREWQGGSVGRMGVGFYGAGT
                     NGSYIGQDDVTMIRGAHTFRMGYEYKQYFYKDDPVENGGAFTFSYRSTDLPAFSTQTG
                     HAFASFLLGAVNNANRNIFGGINDFRQPQHSLYFMDDWKVTPKLTVNLGLRWEIIPSF
                     YEVNNQMSYIDMGLPNPEAGNLPGALRFGTKPSDTYWKEIGPRFGFAYQASNKMVVRG
                     GYAITNTPPIRNDWGYGGFRYGFDAGVNVRQGTGATGFVDDPAMYLTDTFRPFAGTLP
                     DTNPSAGNFDASTTTARDANRPGYVQNWNLTVQYQLPSNTVLELAYIGNKGTRLWGSF
                     VFGEMNGLPASLLQYGDLLREQVADHPTLKPFADFPDDYTVSQAMRRFPQYFGVGEAF
                     PYNSNSLYNSMQVTVTRHLTTGLGFLGAYTWSKTLTYVDSNGPGAYYATVQDYFNRGL
                     ERSVASFNYPHSFKLTWVYEVPFGKGRRWDLGPANYVVGGWQFAANQQYRSGDPLSIG
                     QGGLDIPDGFSSGIRPDATGANTTLGGAPGKTDWSEPTPYLNPAAFAESPRTGAGVPL
                     RVGTAPRVLPSTRGPHSMSENFRMTKSFPIYERIKFQAGITMTNPLNRTNRYIVSTTV
                     GDSGYGQLLQGGGGRTLQIDGRFEW"
     gene            complement(46329..47615)
                     /locus_tag="VLE22_25310"
     CDS             complement(46329..47615)
                     /locus_tag="VLE22_25310"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB17792.1"
                     /translation="MRRREFVTALAAASGAPLSSVPSFGQRSPSDKLKITAVEMWRLE
                     GHRDTIVGINRQQHSNPLHIYEEHRPKPYHEPAEGKPARVATSALYLKIKTDAGLEGV
                     YGPIDKEAAIVVEQQLKPFVIGKDPLAGEKLWDQMFRSDRHARRGHFMMAISAVDNTL
                     WDLRARYYGVPVYRLLGGPTRPAVEAYGSCASMSVEPEAARRRAAALKSEGFRYQKWF
                     FAYGPGDGTDGLAKNVALVRNLREAVGDEVELMFDAFMGWDVTYAIRWAKQAEQYRPR
                     WIEECFPTDKIESFAQLRRATSIPVASGEHIYNRWEVQDYLKAGALNVVQADPEWCGG
                     ISELVKICTVASLYDVQVIPHGHSLHAALHVVASQPPITCPLVEYLLTKMEPYHHFEK
                     EPLRPTAGKITLPEHPGFGIDLDPAKVEKQTRVTWD"
     gene            complement(47663..47818)
                     /locus_tag="VLE22_25315"
     CDS             complement(47663..47818)
                     /locus_tag="VLE22_25315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17793.1"
                     /translation="MRKKLKFDRKKEVRRLARERVGTVPSARIIEPKTKRKRPKHKGA
                     ILEEDAE"
     gene            complement(47803..48618)
                     /locus_tag="VLE22_25320"
     CDS             complement(47803..48618)
                     /locus_tag="VLE22_25320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HSB17794.1"
                     /translation="MFRLTVECRAEERDGLVAELSDAGTEGIVEHDLFEGGCRLEAFF
                     EDMATAAAVAGRISASNVTVEACEDRDWVAYSQAQWEPELVGERFFLVPSWRNDPTPA
                     GRLRLVMPPGSAPGTGFHPATQLALEALERRVRPGRRVFDLGTGSGILALGAILMGAN
                     SVIACDIETAAVEAAASYLREAGVPVSLFAGSARSVRSGAVDLLVANLNAAAIRQLAG
                     EMARVLAPGGEAIVTGFERYEAERVEHLFASFGFACHDRIESSGWVCLICGRS"
     gene            complement(48624..49334)
                     /locus_tag="VLE22_25325"
     CDS             complement(48624..49334)
                     /locus_tag="VLE22_25325"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB17795.1"
                     /translation="MIKTALSLLTCATLVLAAGPRLGPKEKLFNGKSLDGFYTYIEKY
                     GKNNDPKGVFRVENGQIRASGEEYGYFITEKEYEDYYLLVEFRWGSKTFPPREGNARD
                     SGILYHAGGEDKLWPQSIEFQMIEGGTGDLILVGDTAVTVKGETKRRGRFDRFGKGKW
                     EDVAGYRDPNGDPEKPHGQWNKLELWADGDNFRFLVNGKLVNEGSKSSVTRGKILFQS
                     EGAEVFFRKIELQPILGR"
     gene            complement(49331..50875)
                     /locus_tag="VLE22_25330"
     CDS             complement(49331..50875)
                     /locus_tag="VLE22_25330"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HSB17796.1"
                     /translation="MVLPLTPIRFLYRAADLYARKAAVISGDRTFTYAEFAERCERLA
                     SGLGAVGVKPGDRVACLCFNRHQLLEAYFGVPMVGAILLPLNVRLAASELSQLLEHSG
                     ASVLLYDGEFAPVAEQARAVCPPLKCALPLDGDSGYEELLAAGSARRIDWTGIDENSP
                     AELFYTSGSTGTPKGVLLTHRALYLHGKSVALLMPEGDRTVVLQTIPLFHANGWGYPQ
                     AAALLGATQVMVRRFDAETVFVLIERHRGTYMCLVPTMAGALLAAPGRRRFDVSSMKH
                     ILIGGAASSPELIERMEQAFQCECLASYGLTEAGPVVTVGRPDNTTAPDSERYRIQAM
                     AGTPIAGVELRVADSGGSDVPRDMTTAGEVLIRSDHVMAGYYNDPVGTEAALRDGWLY
                     TGDMAVWDDEGRIHIVDRKKDIIVSGGENIAPIEIERAITAHPDVAECAVVAAPDEKW
                     GEVPVAVIVPRPGATITAGDILELLRGRIAKFKMPRRIEFTDTPLPKTGSGKIRKTEI
                     RARIQG"
     gene            complement(50886..>51500)
                     /locus_tag="VLE22_25335"
     CDS             complement(50886..>51500)
                     /locus_tag="VLE22_25335"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HSB17797.1"
                     /translation="PLNVPDTSGKMKSFDDFTFEAASWTLTAHEARPGHEMQFASIVE
                     QGVSTARAVFAFNSTNVEGWGLYAEAVIKPFLPAEGQLIALQHRLLRAARAFLDPELQ
                     LSRITPEEAKRVLLEDVVMSDAMATQEVERYTFRAPGQAPSYYYGYTKLMELRQEAEK
                     AMAGKFDAKRFHDFVLAQGLVPPSLLRSAVFEEFVPQAGGAAGR"
BASE COUNT         9739 a        15591 c        16248 g         9922 t
ORIGIN      
        1 cggccatgaa cgccttccca ctgtttctcc tcaaggacga accgggttcg gtcggcaagt
       61 gcgtgctccg ccttttcgca agcacttcgc atgatgaact cggtgacgtt gacgccctgg
      121 cagtccgcgg ccactttgat cagttactcg tccgatgcgg tagcgcggag ttggaaccgg
      181 cgattgcgag gtcgttcggg cgttgagatt gccatggatt gcctttttcc acattgtact
      241 gccggcgtac tccagggacc agtgggatct gccgtcttga tcgggccaac tctgagtcgc
      301 gggggaggca gtatactgtg tggcagcttg cattgggagg cgatatggtt cgcaaactca
      361 cccgaatcgt tgctctagtt gctctttgct ccctcttcgt tgccgcggat gatagcggca
      421 agcgcgtgtt ctccgtcatg acccggaacg tggataacgg caccgacctc ctgtacgtcc
      481 tcggaggcgt gcccggcgca atcagcttga ccttcgacga ggtcgtcgcc agcaatatcc
      541 cggcgcgttc ggcacggttg gccacggaga tcgccgccga gaggccggac ttgatctcgc
      601 ttcaggaaat cagcgtctgg accaaacttt ccgcggcggg ccagccggtg ctcgtgctcg
      661 acctactgga agaactcatg gctgcactcg aggctcgccg gttgcgctac gacgtcgtgg
      721 ccgtgaacaa tctcgccaat atcacgcagc cgctcgaaac cggagacggc tacgtgaacc
      781 tgctcgatcg cgacgtggtt ctggcgcggc ccgagctgca gatctcgaac gtcagcacca
      841 acctgtatgc ggtattcctt cccttcggcc cgtttccggt ctatcgcggc tggatctcgg
      901 ccgaggtcaa cgacgggcgg aagaaattcc ggttcgttaa tacacacctg gagagcctgc
      961 tgacgtcgat tcaagtggca cagaccggcg agctcatcca ggtcttgtcg caggacgacc
     1021 tgccgctggt gctcgcgggc gacttcaact cggacgccag cggtaatccg gcagcgctgg
     1081 ataagcccga cagcgtgtta ctgctgcagc aggccggatt caccgaggcc tggcaggcgc
     1141 ttcacgcgaa cgatcccggc tacacgtggc cgctgttcct cgaggaccat ttcccggcgc
     1201 cgccattcac ttcgccggcg gcgccgttcg agcgcatcga cctggtcttc gtgcgtgggc
     1261 cagtggaaat ccgcgccgtc gaacggaccg ggctcgttgt accgtacggc tccgaccacg
     1321 ccggcgtcgt ggctacgctg aagctcgagc cgtagttgcg caaagccgga cgcgtcgatg
     1381 gtacgatgag gtttcaatcc cattgttctg atgaaaaacc tcgtgtgtct cgccggcgcg
     1441 ctgctgtgcg gcgcctcgct ccatgctcag acccagactc ctccggctcc tcagcaagag
     1501 gtgcggcgga aagcttatgt ccgccgtctc tcgctcggcg ccacgctcag tgtcctcggc
     1561 cttcgtctcg tgcccgaccg cacaacgtcg gtggtgacgt cgtcgcccgc gtttgacgcc
     1621 atctacacaa caacaggcgc gtcaaagcgt gtcgggtacg gactcaatct ccaggcggcg
     1681 gtcacagacc gtttcgcagt gaacgcgagc ctgttcctgc gcaaagccgg atacaagatg
     1741 aacagcgaca tcattgaggg cacggacgat cccaatacca gcgccgacga acgcacgctt
     1801 accgtgcgga atgaagacac ccgggtcaag ttttacgacc tccccgtcct cgttcgctac
     1861 tacggcaaag accgtcatca gaggggtccg cgctggtttg tggaaggcgg cggcgttctc
     1921 cgcagggtgt ccaatatccg tacctcgatc gacacaacaa ttggcaccgg cgataaacag
     1981 tgctgtgata ctactccaag gactccggaa cggcgaacct tgcggggatt tgtcgcggga
     2041 gccggagtgc agttgattga cccggtcgga atccgcgttg tccccgaagt gcgctacacg
     2101 cgctgggcgg gacatacgtt caacgcgttc tccaccatca ctgagcgcaa ccagatcgag
     2161 ggaatgatct cgctaacttt ctgacgaacc cactgaagtc caaccaggga accgctaccg
     2221 cggaggggag ggcgagaggg gcttcgtttc ccggaatcgc tttcgggcct cgtagagcac
     2281 aatcctaccc ttgatcgcgt ccgccagaga tccgttgttc gacttctcag cgagcgcaag
     2341 agcgcggcgg gcggtttgcg tggcagcgga atatcggccc gcctcggcgt aagcggcggc
     2401 gagcgtgtcg aggatctccg gatcccggcc gccggacaga cggacagcct gttccgcgaa
     2461 tacgacagcc tcggctccgt ttcggaccga agccttcgga gacgttgcca gagcccaggc
     2521 cgcctgggtc agtactgaca cacgcgtggg ttccaggcgg agtccctcgc gccattgagc
     2581 gacagcgtcg aaggtcttcc cctgcatgta gaaagcgctg gccaggttaa agcgggcttc
     2641 ggcgaactcc gggttgattt tcagcgcctt ttgaaactgc gaggtcgctt cgttgaagcg
     2701 ctctttacgc agtaggaggg cccccagatt gttgtagctg gccggcgacc cggggttcac
     2761 ctccagggcc cgctcgaaat gagaaatcgc ctcgtcgagc ttcccccgcc gcagcagcac
     2821 cgcgcccaga ttgctatgcg cccggccgtc cccaggactc attacgagcg cctgcttcca
     2881 ggcagcgatg gccgcgtcgt cctggccttt ttccgtcagc tcccaggcac gctcgtacag
     2941 gcggtagaat tcgaccgccg gcacgtcgat cttcagcaag ccttcggggg gtatgttcac
     3001 gaactcgggg atgttgaccg cccggttcgc cgccgttgca ttgtcgacca ggatgggcgg
     3061 actgtcgcgg ccgtcctcgt ccaggtgagt caggtacatc tgcgtgtacg gcgagcgggc
     3121 tttggaggaa aagacgagcc agcggccgtt gggcgagaag ctgtgccagg agttcatgag
     3181 cggggtatta cagcgcatcc gccgtgcctc gccgccttcc accggaacta tatagagttt
     3241 gctgtcaggc cgcatcaact gaccgtttcg gcactgcacg aagacgagcc accggccgtt
     3301 gggagagacc ttcgggaagc agttgctcgt gccgttcccc gaagcgccgg caatcggagt
     3361 tgcctcaccg cccttgccct cgttgaacgg aatccggtag aggtcgtact gaatcggggt
     3421 ctcgttcgga tcgccggcga attcggccat ttttcgtccg acgggataag gctccctcgc
     3481 cctggccctg gcgaagacta tgtacttgcc gtcggggctc caaacggcat cggtctgcac
     3541 gtagcgaggg gagtcagcgc ccggcaggga ttgaatacgg cgcgagtccc ggctgtacca
     3601 cgcaagtatt ccgcgtgtcg gatagaagac ctgaagaaag cggtagtcct tgaaattggc
     3661 gacatagtaa ctgccggcca gatcggtctt ctcgccattg agagaagtta cgacgaaccg
     3721 cccgtccggc gaaacctgcg acatgaagcc cacgcgcatc ggggacgccg ggcggtcgcg
     3781 aaacgaatcc catgagatca cgtcctcgtt gtggatggac atctgggggg agatcgaggc
     3841 aatcgcgtac atgcccttgt cgttccgggg gccgtcgaga tccaacccta atgtcttgcc
     3901 gtcgagggag aacgaatggc agttggcgca cgtcggcagg ccatccagaa gcaggcggct
     3961 gcggggctcc gcgatgttcc gcagcctcca ggcaaccagc ggtatcgcgc tcgggggcag
     4021 gggtttaatg accccctttt ccaattccga gggcatcaac ggcacatcac ggtaaaagac
     4081 cggtgcaccg acgggatctt ttgaggttcg gatgctgact tgccccctgg aaaccggctg
     4141 atccggattt gtgccccgta aaccggtgat agccaccgtc gcagggtggg tggtcgagcg
     4201 cgtcttgatc gccgcccagg tttcggcgtc tggcgtccac gttcgcgccg cagcctgctg
     4261 cggcgtcagc ttcggcagct cgtttgtgtt cgcgatgcag cggggatcga tttcaccgat
     4321 ctgcaagcgc tcaccagtcg acattgcgcg gatggcggcg gagccgtccg ggaacgtgac
     4381 atcaacgcgc cacagcctgg cgctctccgt ggcgtctcgc caaagaaacg tggggggtat
     4441 gatctccgga gggaagatcg atccttcctc cgggtagtca atcgtgatcg atgcgagggc
     4501 ggcgttagag tgtcccggtg atcgggaagg gtcggacgga cggaccgttt gccgggcgac
     4561 gagcagaccc accgccgtga tcacgacacc gatacccaag atgcgactcg cgatgcgcgc
     4621 cacgagaccc ccagagtctg atgcttcgag caagcctacc gaagcttaga atacaacagg
     4681 acggccgccg ccgaagcgga ggaccggcct gcgaggttac tccagcgccc tcattaagaa
     4741 gtagtcaggg gagtgggcag gagcgtgata aagcgacttc agttcctcgt cgagcttcat
     4801 aagagtgatc atgcagccgg ccatttcctg gcacgtaaag aacggaccgg cgtgcgtcgc
     4861 gtgaacggaa atcccgtatt cggctagaac acggtgcgca gctcgggcca ttatgtacaa
     4921 ctccatgtac gttgtggcgc ccatgctgtt cacgagaagc aggacttcgt caccggcgcg
     4981 gaacggcagg tcggcaacca tacggtcgat catcctgcgg caggttgagt ctgcggaggc
     5041 catcggctcc cgacccaccc cgggttcgcc gtgcattccg atgccgattt ccatctcgga
     5101 ctcgccgatc tcgaacgtcg gtttgccggt ctgcggcaca gtggcgctgg tgagcgcgac
     5161 cccgagcgtg cggcagtatt ccctcgcacg ctcgatcacg ggaaccatgc cggcaagcga
     5221 agcgccacgc tcggcaagtg ccccagcaat cttgattagg aacacatcgg cagtcatgcc
     5281 gcggcgttcc tctttgcgcg cgggcggagc ggaagcgatg tcgtcccaaa cccgcaactg
     5341 gaacgtgggg atcccttctt cggcgagcat ctcgcaggcg atccggaaat tcatgttgtc
     5401 gccggcgtag ttgccgtgaa tcatgcaaac gccggcaccg cgatgaactg caccggcgcc
     5461 ggcgacgatc agatccggcg acggggcgga gaagatgtcg ccacagacgg cggcgtccgc
     5521 catgcccggc ccgagagcgc cgagaaagag aggctcgtgc ccgctgccgc cgccggtgag
     5581 aacggcgacg cggccatctg gaatgtcgcg gcggacggcg actgttcctt cgcccacccg
     5641 gataaggttg tactgcttcc cgtatgcggc aaggaagcct tcgaacagtt cttcccgtac
     5701 tctgttggga tcattgatca actttttcat gctcttggct cctcttcgcg aatcgcatcc
     5761 gcgacggcgg tgagaatgat cgccatggta tgcgctcctg ggtcgggatg gccgatggac
     5821 cgctcgccga gatagcgggc gcgcccttgg cgtgcgacga gtccggcagt ctcacgtgct
     5881 ccacctgctg cggcctcggc tgcccggtgc atcgcacgtc ggagtggcac ggcgtcatcg
     5941 gcatcacgac ggagcgaatc gactgccgga gcgagtgcgt ccaggagtgt cttgtcgcct
     6001 ggccgcgcgc cgcccaggcg agtgactccg tccaggctcg cctgtaacat caccgccatg
     6061 gtgggtgtgt ctatctcgcc gctgtcaccg caggctttac cgagctcgga gaagaaagtg
     6121 gccagaatcg gaccgatagc tccgccggtg gaggagagca agactctccc acactggcgc
     6181 agaatcgggc cgggggccag ttgcgcccac gcgaggatgt tttcctcgac cgcctcgaat
     6241 ccccgggcga cactcacgcc gtgatcgccg tcaccgcagg cagcgtcgag gcccgaaagc
     6301 cagtcctttt gttcccgcaa gcgctcaccc atgagagcga gggcgcggat cagcgccgga
     6361 gcgtccatag ctgtcgggac attctatcgc tatcggctcc cacggcaggg aacacaaatc
     6421 ccgcgagtac agcaaaaagc accggctgcg gcgtcagttc atcgtgggct tgatccaggt
     6481 caactggacg ccaccgaaac cccgagccaa aaccaattca ttgccgtccg agccgatcag
     6541 aatgccgtta tcgcgcggcg tctcccgcat tatcttccag tcgccgcttt ttcggtcgag
     6601 gcgcgcgagc cggaagtgct tgtcgtctgt ggagcagctc cagcagaggg catagagttc
     6661 accattcacc gtaaatccca atgagtattt gaaacggctg tagtggtcgt cgaactcagc
     6721 gccgtcaatc ttccgaccca gcggaaccgg accgagagtc cactggccga cgatctcgcc
     6781 tttcaggttc agttccgtcc agacctctgg tttatcgtct atgcggaacg acagaattcc
     6841 aatcaggccg tgagcggcgc gcattttcca gcgtccgtgg cctccagaca caaagccctt
     6901 gtccaccgac gtgcgcttga ccgctccgag gatcagtgtt ccgtcaagtc cgtactttcg
     6961 gatcaacggg tagtcggctt tgtcttcttc caatatccgg tctgcgtcac gctgccagcc
     7021 agcggtccag atccggtcct cttcatcgag gcagatttgc agtggcatgt acaggccggt
     7081 ctggatgaac cggcggatct ttcctgctgg gtcgaggatc gcgatggccc cgccgtagcc
     7141 tctttcggcc ggcgtatgga tcagggctaa gacagccgta ccgtcccgtt tcatggcgac
     7201 gtcactaatc gagacggttt cggtgccggg gagcttgagc tcaatgtcgt gaatggactt
     7261 accgtcgcgg tcgtggacga tgaggtaatt tcggttgcgg atggcgatca gaaaaccacg
     7321 gtccacaacg accatgggct gggtgaagga gttgctcata gcgacggtgg gatagaagac
     7381 ctcccggacc gagtcgccgg agtgttgtgc gatggcgggt gaaggcggaa ggcagagtgc
     7441 tgcaagaacg aaccaaggag cttgatagcg catggagcgc ctctttacca ggatatgaca
     7501 cacgaaacca cgcgagcgtg gaggcagggt tgcaggctcc gctctgattc ggcgtgcgcg
     7561 atttccttac ggtataaccg gcagcacgat atgcgaggcg tgctgcgggt cgtggtagac
     7621 gctgttttcc gcaatcgccc atcgacggtt gttattaagc ggctcgccag tgtttgggtt
     7681 caggtcgaag cgcgggaagt tgctgctgga gatatcgagc cggatacggt ggcctttgcc
     7741 gaaaacgagg gaggtcgggt acatctcgat ggtcaccttc gtgatctctc ccggctgcat
     7801 cagcttcgaa agctcgacgg attctcgata gcgggcgcgg atgatgccgt cggcgacgtt
     7861 gaggtccacg ccggcgggga agtccctgtt gggcgggtaa acatcgacca gtttggcggt
     7921 gaagtcggtg tccggcgcgt tcgaagaaac ccagaggttc acaatgaggc ggccggtgac
     7981 ttccatcggt gattccagcg gatcggtctt gaaaaccaag acgtcattcc gtgccgagag
     8041 cggcagggta tccttgcagg accagagttc cttgcgacac ttctggtcgg ccgcgccacg
     8101 gggcatcagc agtccttcgg atgacacgtt gccgccgatc gtcggcaccg ggtctcgcgg
     8161 atcgaagaga taccgcgtcg gaggagcctg cactggcggc gccgccgcca gccgtccgcc
     8221 ggtgtgaagg tagaacgatg tcggcttcgc cctcgccaac ggccattcgc gctcgtctcg
     8281 ccactggccg ccgacaaaca cccgcccttc ggtcgtctta tgcccgtcgc cgcctcccat
     8341 gacgaagatg cgtaccgggg tgtcgcgatc cacaccgttg tccgaaccct tcagccacca
     8401 gtcgaaccag cgcaagcgca gcgcgttaaa gtcaatggcc gcgtccgggc cgaattccgc
     8461 ttcgccggcg aaactggcgg tctgcccgcc atgtgtccac gggcccatga tcaaccgctg
     8521 cgggctcttc ttcgcgcgcg cgagctccac gtagttcagg ttggcgactt gcagccccca
     8581 agagtcatac cagccgctga cgtggtagac gggaatgtcc ttgtattcgg cgatatggct
     8641 cacgacgtcg gacccgcagt tcttccagaa ctcgtcgtag tcgccatggc tcattgcctc
     8701 gataagccag ctttcatact ccggagcaaa gcgcagcggc gttgttcccg gacggagcgg
     8761 cagcgctttg atgtattcga tgacgtggtt tcgcaggtcg gcgagggcgg cgcgggtagc
     8821 ggggtcggag gtcgggtagt gttgcgggac cgtcgggccg tagcctccgc tgggactgtt
     8881 gccgagggag aagatccagt tgaagaaacg cagctcgaag gcgccgtggt gacgcactcc
     8941 gtacaggccg gggttggaca tcgaatccac ggggaccatc gctgcgagat acggcgggtt
     9001 gcccattgca agcgcatgct gggttcctcc cggatacgaa gtgccgacgg tgccgatctt
     9061 tccgctgaac caaggctgct tgccaaccca ctcggcggta tcgtagccat cgcggacgtc
     9121 gtcgcgatgg gggaaccact ctccttccga gccgtagcgc ccgcgcacgt tctggccgat
     9181 ggcgatgtaa ccgcgcggga cgaagtacga cgcccaggac tcgatcgtgt gggaaccgta
     9241 cggcgtgcgc tccagaatga ctgggaactt gccgggaacc gggacaccgt tgcgggcagg
     9301 gaagtaaatg tccgtgctga gtcgtgcgcc gtcgcgcatc gggaccatga ccttcttcgc
     9361 cgtcacgatg tcgtactgcg gtgtggattg ggccgcggcg agggccgcaa tcacgagaag
     9421 gaaagcaagc cgtcctgtca tgggagccag tgtatctctc cgtgcggatt ttcacgtggt
     9481 agactttccg aaacgcagct cagaccgagc gcgcattgga cgtcaaggag ggcaccatga
     9541 aagaaccaga acgtggcaag gagatcctgt acctcacccg gcaagaggtg atcgacatcg
     9601 gcctgaccaa gaaggacatc ctggaactgg ttcgggcggc gctgaccgag cacggcaaca
     9661 aacgctgtga gatgccggcc aaaattggca tccatccctt gagagacacg ctgatgcacg
     9721 ccatgcccgc ctgggttcca gcgcgccagg cctgcggaat caagtgggcg gaatgcttcc
     9781 ccgacaacta cgagaagtac ggcttgccgc agacgagcgg gctgatggtg ctgaactctc
     9841 cggagactgg ctggcccatc tgcattcttg acgcgatctg ggtgaccgcc aagcgcacgc
     9901 ccgccgtaac tgcgcttgcc tgcgagtcgc tggcccggaa agataccgcg gtggtgggaa
     9961 tcgccggagc gggcgtgcag ggcagggaac acgtgctggt gctccctgag gtcctgccga
    10021 agctgaagaa gatcaaactc tacgacaagt tcccggaggt tacgcgaaag ctgattgctg
    10081 accttcagcc cgaggtgccc ggggtcgaac tcgtggaagt cccgggtttt gaggagctgg
    10141 tccgcgggtc gctcgtcgtg gtgacaggaa cggcgatcct gatggcgccc gatccgcaga
    10201 tccgcgacga gtgggtggaa ccgggggcgt tcctcgcgcc ggtggacttc gacagcctgt
    10261 gggagtgggc gacgttttcg cgagccgaca agttcctggt cgacagtctg gacgagatga
    10321 agtacttcat gacggtcggc tatcttcctc acggcctgcc gcctctgcac gccgagatcg
    10381 gggaggtggt cgcgggacta aagccggggc gagaacgcga cgacgagctg atcatcgaca
    10441 tgaacatcgg catgggcgtc gaggacgttg tggtcggacg ggagatctac cggcgggcag
    10501 tggcgagcgg cgccggaacc atcctgccac tgtgaagacg cgcgggctca gctctcgacc
    10561 ggtacacgct cccagcgggc gataacggca gtggcgagac agttgccgag gacattcacc
    10621 gatgttcggg ccatatccat cactgcgtcg atgccgagca gcactgcggc gccctcgatt
    10681 ggcaactgaa acgttgccag cgtgccggcg agaatcacca gcgcggcgcg aggaacgccg
    10741 gcgacgccct tgcttgtgag catcagcgtc agcatcatca gtagctgctg gccgagcgtc
    10801 agctcgatgc ccgccgcctg tgccacgaaa acggaagcca gcgacaggta aagcgtgctg
    10861 ccgtcgaggt taaagctgta gccggtcggg atgacgaaag acacgatgtg cttcggcaca
    10921 ccgaaccgtt ccatgttctc cagagcgcgg ggcagggcgg cttcgctgga tgcggtcgag
    10981 aaagcgatca agaacggctc ccgtgcggcc cggtagaagc gcccgaccgg cacgcgggcg
    11041 atcgccatca cggcgccgag aaccaccacg acgaaaatcg cctgcgtggc gtacatcgtc
    11101 aggatgagtt tgcccagccc gaacagcacg tcgagtccct tggagctgac ggtgaccgcc
    11161 atagcggcgc agacgccggc aggcgccagc cacatcacgt agccggtgta cttgaacatc
    11221 acctcggcga gcgattcggc aaaatccacc accggcttcg ctttggcgcc aatggcagcg
    11281 caggcagtgc cgaacaggaa cgcgaagaca acaagctgga gcacctcacc gcgtgccatc
    11341 gcgtcaaaca cgctcgccgg aaagacgtgc tccaggaaat tgccaagggt tggcggctgc
    11401 acggcaacag tgctttccga cgcggtccgc tcgagtttca tgcccgcgcc gggctggacg
    11461 aggttgacgg cggcgagacc caggaacagc gcggctgtgg tgacaatttc aaagtacaca
    11521 agggccttaa ggccgatccg gcccatcgcg cggatgttcc ccgtgccggc aatgccgtag
    11581 acaagggttc caaacagcag cggcgcgatg atcgacttga tgagccggag gaagatgttg
    11641 ctgacgggcg caaggttgag ggcaaagccg ggagcgaaca agccgaatgc aatgcccacc
    11701 gccattccga tgaagatcca ggctgtgagt gagatttgtt tcatcgctca ccccagcgca
    11761 gtttttcgcg gagcacgtcg aagaacagca tccggggcgg acgaatcagg tgaatgtagt
    11821 ggctagaact gctgcagatg acgcggtcat cccgcatgag cggctcaccg acctggccgt
    11881 cgacggtgag ataggtttca ttgtcatcac cgtgattcca gacacggatg acgctcgact
    11941 ccggcacgat cacgggacgg ttggtgagca tgtgcgggca aatgggagtg atgcacaacg
    12001 cgccgacgga cgggaagata atcggtccgc ccgccgagag cgagtaggcg gtggaaccgg
    12061 tcggcgtgct gatcaccagg ccgtcggcct tgtagtgaca gacgaagtgg ccgtccacga
    12121 atgcctccag gtcgatcatg cgggcgatcg aggccttggt gacaaccacg tcgttgagcg
    12181 attcataact cgaaaccgtt cgctcgccgc gtaccaggtc gcaatgcagc atgcggcgct
    12241 tgccgatgcg gtgctcaccg cggagggccc gctccagttc cggatagagt tcatccgtcg
    12301 agatggcggt caggaaccca agacctccga ggttgacggc gaacagcggg atctcccgcg
    12361 cggaaacggc gcgggcggct gcaagcaagg tgccgtcgcc gccgagcaca acgacgagat
    12421 cgcagccctc cggagtctcc gcgcgggaaa aaccatcggt acggcccgcg tacgccgcgg
    12481 tgaactcgtc gcagcgcaca tggaggtccc gggaggccag ccactcgatg agtgccggga
    12541 cgatcgcgga cgcctggcgg acattcggtt tagagatgat tccgatcgtc ttcatgccgg
    12601 gcatagagca gaaattcctt gtttccttcg gcgcccagaa tcgggctgtt catcagggtt
    12661 gtccgaaagc cgagcgcgcg cgcggcacct tcgacgcggg cgcacgcggc ctggtgcagt
    12721 tccggatcac ggacgatgcc gccctttccg acttcgccgc gtctaacttc gaactgcggc
    12781 ttgacgagta taaccatttc ggcctctgct tgcaggatcg ggaccaccgc gggcaggatg
    12841 agcgtcacgg aaatgaagct cacgtcgcag accgcgaggc cggcgcgctc gccaatctcc
    12901 tcgaagcgga ggtatcgggc gttaatgccc tcccggacaa tgacacgggt gtcgttgcga
    12961 agtctccaat cgagctggcc ggagccgaca tcgaccgcgt agacacggag cgcgccgcgc
    13021 tggagcagac aatcggtgaa gccgcccgtc gatgctccga tgtcgaggca gacacggccg
    13081 gttacgtcga tgccaaaccg atcgagagcc gctgccagct tgatgccggc gcggctgacg
    13141 taaggcagac gctccaccag ttccaaccgg cattgggtgg agaccgtctg tcccggcttg
    13201 cccgccttct gtccgtcaat caggacatgc cccgccagta ccagcgcctt cgctttctcg
    13261 cgcgattcgg ccagaccgcg ttccaccaga agctggtcga gtcgctgcct gaagacgagt
    13321 ttcatgcctt acggtggacg atcaggtcgg cgagctcgcg gaggcgcgcg gcgcgatccc
    13381 cgaacctctc gagaacccgg tgcgcgctga gacgttcctc ctcggccatg cggtgagatt
    13441 ccttgaggcc gtaaacggca gggaacgtga tcttgtgctg ctgtgcgtcc ttgccggcgg
    13501 tcttgccgag tgcttctgac gactgctcga cgtcgaggat gtcatccacg atctgaaatg
    13561 ccaggccgac gtgctcgccg tagcaggata gggcctcgaa ttgcatgtca tcggctcctg
    13621 cgtaaatggc gccgattcgc aggctggcgc ggatgagcgc tccggtcttc gcgcggtgga
    13681 tggtttcgag gagtttcggt tcaggggcct ggtgttcgcc ttccaggtcg gcgacctgtc
    13741 cggcgaccat tccgcgtggg gtgccggcgg cggacgcaag ctcccgtacc atgcgtgcgc
    13801 ggcggtcgag aggtaccgtg ctgacggagg cgaggacctc aaatgcgcag gtgagcaggg
    13861 cgtctccggc aaggatggcc atggcttcgc cgaagacttt gtggcaggta ggcttgccgc
    13921 ggcggaggtc gtcgttgtcg agcgcgggga ggtcgtcgtg gatcagggaa tacgtgtgga
    13981 ccatctcgat cgcgcaagag gcggcttcga cgccttcgca ttggccggag acggcgcggg
    14041 cagcctccat gcaaaggacg gggcgaatcc gcttgccgcc ggcgaagagg ctgtagcgca
    14101 tggcgcgatg gatgatcgaa gggggcgtcg actcggccgg tgtccaccgg tcaagctccg
    14161 cgtccacgag gcggatctgc tcggtgaggt attcggacag gatcattcgt tttcgggatg
    14221 gaacggttcg ggctgcactt tgtcgttgcg tttgagaagg atttcgaccc gcgactcggc
    14281 ctcttccaac tgcttgcggc aggtttcgct gaggccgacg cctttttcga agagggcgag
    14341 agcgcgttcg agcgggaggt caccggcttc gagttccttg acgaccttct ccagatcctc
    14401 gatactcgct tcaaagctgg cgaggggctg cttttccggc tcgggcgtct tggccatcgc
    14461 cttattttag cgaagatgcc ggattgtcga gtttagtaag catcagcttt ctgtttcgaa
    14521 ggcgcttctc gcaacgccgc tctgccggat gatgctcatt agcgttcctc gcttcagctc
    14581 ggcgtggagc ggaacaggta cggtgacagt gctccccgcc gatcacctct gcatgacgct
    14641 gtgacttccc cgacttcggg cctccctgaa accatacccc tcgaggatgc gacacacctc
    14701 tgagccggac aacgccctca gtttaggcaa cgcggacctc aacctgcgtt accaagacct
    14761 cctgccggag gcgatgctcg acctcggatg gatcggcggt ttcgaagaaa agggtcagag
    14821 cttctacaag gttcgtccga gcctcttcga tcgtgttccc ctggctggcg atgtcgagct
    14881 caggacaaag tgcgacgtaa acatcatgct cgcgctcgat cactgcggtc acccgacgta
    14941 cttgctccat aggactcatt gtagacacaa gccaagcatt ggagaactac caacgcgtcc
    15001 gcatgttgtc gccgctacat cccggcccgc tttgccgtca cctcgatctc gatctttata
    15061 cgagggtcga tgaggccggc gacgaacatc gtggcggcgg ggcgaatgtc gccaaggtac
    15121 ttgcgtagaa ccggccagca gagagggaag tcttccgcct tcggaaggat gtaccgcatg
    15181 cggaccacat cgcggagcga acagcccgcc tcggcgagcg cctgctcgat gttgcggaag
    15241 cactgctcgg tctgttcgat caggtcgtcc gaaatcgtca tggcgcggta gtcgaatccg
    15301 gtggtgccgg atacgaagac ccagtcgccg tcaaccacgg cccgggagaa accgatctcg
    15361 gcttcaaacg tagaacccga tgagataaga cgacgcacgg cgattgctcc tgaaattatg
    15421 gcagccggag gggactgtga cttgcgtccg gtggtgtcac gccgagccga tgggccgctt
    15481 tcaccagctc ttcccagacg cccgcgttga gcgggatgcc ggtcctgcgg cgatcctcct
    15541 cggcgcgcca ttcgggctcg ccggctacga gcacttcatc gtaacccgcg gcgggcgcgg
    15601 tcgccttcac ccgagagacg agttcgtcga gccgggcacg gaactgttcc aacggcatga
    15661 agcgcgctac atcgatcgcg atgaacgtct ggctgattcg cgtagcgcag ccgcgggtgc
    15721 gtacgccgcc gacatcggtg ctcatcgccc cgccgccaag cgcgccgcag aagatctcga
    15781 ccatcatccc aagaccggaa cctttgtagc ctccgagagg catcaggagg ccgccgtgca
    15841 tggcggtctc agtgtctgtg gtgggccgac cctcggaatc catcgcccag ccaggaggaa
    15901 tctctggctg gccgctcatg gcggcccgat agattttgcc catcgctacc gtcgtagtgg
    15961 ccatgtcgag gagccagccc cttccgccag tgcctgggac cgtcacgcag atcgggttcg
    16021 ttccgacgcg cccttcacgc ccttgccatg gtgccaccaa aggcgtggcg ttgcacatga
    16081 caatgcccac catcccttcc gacgagatgc gctggcccca gaacgccgct gcgccaaagt
    16141 ggtttgaatc cctcgcgatg acgatgccgg cgccgtgctc gcgcgccagc cggacggcat
    16201 gaccacaaca tctctccgat acaaccgccc cgaggccgtt ctcaccgtcg tagttgaggc
    16261 aggctccgct ctcggagatg acatggcctt cagccgtcaa gttcacgcct cccgcttcaa
    16321 tttgctccac gtaaaagcgc agcaattgga cgccgtggga atcgacgcct cggaggctgc
    16381 ccgatacgag gcactccgcc ataaccgctg cgttcggagt gggagcgccg gcggcttcaa
    16441 gcaactgccg actgaaaacg gtcagtactg agtcggggaa gtagagcgtt tcttcagaca
    16501 tgttaattga gtgcttactt ctgtaagtgc ttgttaatgt taggtcgcaa aagttgttga
    16561 atctccatag tttgtaagtt ttcgggagac acaaccgttc cgccgagatc gatgcgtttc
    16621 ggcgggttct ttccgtgaag cttgtctacg agcgtcttca cggcttcgta gccgatacgg
    16681 aaaggatcct gcacgacagt ggcgtggatc acgccggact tgatgtcctc gatcatggcg
    16741 tcactcgtgt cgaaagcgac gaacttgacc ttgccgccga ggccgcgcgc cttgagtgcg
    16801 agggaagcgc cggacgaact gggctcggtc gatgcgaaga tgccgtcaag atcggggtgg
    16861 gccgtgagga tattctccgc tgcggcgacg gacttggcgc ggtcggacat gccgaattgc
    16921 cgggcgacga cgcggatatc ggggaattcc tttgccatca cgtcgtcgaa gccgcgctcg
    16981 cgctccatgg tggagtagct tccgggggcg tgcatgacga cggcgacctt gccgcggttt
    17041 ccgaggaggc ttgcgagctt ccgggcggcg agctggccgg cctcataatt gttggtggcg
    17101 acgaaggtca tgtaattctc cgagtcgagc ccggagtcga agatggtcac cgggataccg
    17161 gcggcagcgg cacggtcgac cgcctggacg agcgccgtgc gctcagcggc ggcaagagcg
    17221 atgccgtcca cgcgccgggc gatcatcgag tcgacgatct ggatctggcg gctgtattcg
    17281 ttctcctggt tgggtccgtt ccacagcaca tccacgtcga gctcccgccc ggccgcaaga
    17341 gcgcctgctt gaaccgagac ccagaaaagg tgagcggtgc cctttggaat gacggcgatg
    17401 actttcttac ggctgccgcg acagccggcg agcgcgacgg cgatcagaag aacgaaggtg
    17461 gctgcacgcc gggtcattgg gcgcaccagc cgccgtcgat gagaacgtcg gtaccggtga
    17521 cgaaacccgc ttcttccgag cagagccaga cggcgaggcg gccgacatcc tccaccttgc
    17581 cccagcggcc aaggggcacc tttgccatga actggcggtt gacctcctcg tcctgcatga
    17641 gaacggtgtt catctcggtg gcgaatgggc ctgggctgat cccattgaca gtgatgccat
    17701 cgggcgcgag ttccagggcg agagctttgg tgaagccgag caggccgctc ttgctggcgc
    17761 agtacggcgt gcgtcccggc aaggcgacgt ggctcatggt cgaggtgagg ttgataatgc
    17821 gtccgtagcc gccgcgcttc atgtgaggaa cgaatgcgcg gcacgtaaga aaggcggcga
    17881 cgaggttggt gtcgatgatg cggcgccact cctctagggt gaagtcggta atgggcttcc
    17941 tgaggtttac accggcgttg ttgatcagaa tgtgcaggcc gccgaaccgt gtgatcacat
    18001 cggtttcgag gcgcagcacc tgagcctcat cggagacatc ggcacggaaa acttccgcct
    18061 cgccgccggc gttccgcacg gcggaggcgg tctcgttaag cttttcgaga tcgcgggcga
    18121 ccagggcgat gctcgcgccc tccgcggcga aggcagtcgc gatggccctg ccaagtccgc
    18181 ggctggcgcc ggtgatcagt gccactcgtc cggcgagttt cctgtcgctc acttctattt
    18241 ctccttgtcg gtcgagagat gggccgcgcg gctgacgcgg tcggcaacgc cgtgtgggtc
    18301 gtcgatgtcg tggatggtca gcaggctggt ctcaccggcg gtttcgatgg cgagggtgcc
    18361 aacgccgagg agacgctgaa agagggtctg atcgacacgg acgtcctgca ccttggagat
    18421 ttggatgttc cgggtggttt tggtcagaag acctgattca tggcggattt tgtcgccctg
    18481 gatggtcatt ttcgtgaggc cgcggcggat ctggcggctg agcggccaaa gaaacaggag
    18541 cgccggcgcc agcagcaccc acgccgagac gctctccatc gcggctgagt tggtgtaggc
    18601 cagcacggcc gcgatgaaga tcagcgaaat cacggtgtat cccgcacgga tcagcttcat
    18661 ggtcggacgg atgacgaact cttccattaa cggatctcct aaacgggcta ttgtacagca
    18721 ggggtgcggc ggcgctcgcg caggatgcga tacgataact ggaactggag gggctatgaa
    18781 aagaacgcta cttgcggtat cggcagtgct gtgcgtggtg gcgggcacgg cgcgcgcgga
    18841 cttcagctat gagcagtcct cgaagatcac tggcggcttt ctcgcgggaa tgatgaagtt
    18901 cgccggcgtc ttctcgaaga aggccaccga accggtgaag acctcggtgc aggtcaaggg
    18961 cgaccggatg gcgaacatcg gcagcgaaag cgcgcaggtg gtcgacctcg gcaaggagac
    19021 gattaccgac atcaatttca agaacaagac atacgcggtg atcactttcg cgcagatggc
    19081 cgaagcgatg cagcgcatga tggagaaagc gaagcagcag cagggcgccg agaatgtgga
    19141 cgccaacttc aagatctcgg tgacgccgac ggggcagacc cggcaaatca acggactcga
    19201 cacgaaagaa gtcatcctga agtttgaaat tgagagcaag gacaaaaagt ccggggagca
    19261 ggggtcttta accaccacgt cgaacatgtg gctggcgccg aaaataagcg ggtacgagga
    19321 agtgacaagc ttccatcagc ggatgggcca gaagatcgcc tggacgccgg gcggtatggc
    19381 gctggcgcaa ggccgttccg acatcgcgaa gatgatggga aacgtctcca aagaaaccgc
    19441 aaagatggac ggaattcctg tgctccagat tatgcgcatg ggcggcgaag cgcagggaca
    19501 gccggcacaa gcaggacaac cggcggaggc ggggcagccc gcgcctcagg cggcccccgc
    19561 ggatcagcag gagcagggca gcgccaaggc ggttgccgga gcgctggggc gtttgggtgg
    19621 attcggcggg ttcggccgcc ggaagaaaaa ggcggaggag cagcccccgc agcagcaacc
    19681 ggagcagcaa cagcagcagg cggccggcca gccgggagcg ccggcgggcg gtgttctgtt
    19741 ggagatgacg accgaattaa ccgggttttc ggcggccccg gtggacgcgt ccaaattcga
    19801 ggtgcctgcc ggtttcaagc aggtcgagca cgagatggtg aaggctttgc ggaagtaaga
    19861 ggagggaagg ggggcgcgga gcttcgctcg gctccgcgcc gatctgaatc tattcgaagt
    19921 cgcggggtcg cttcttgcgg ttccgcttgc gggcgagcgc ggctttgaca cgcttcttct
    19981 cgcccggctt caggtagtag gagtgacgct tgatctcctt gatgatgtcc tcctgctgca
    20041 ctttgcgctt gaaacggcgc aatgcggctt ccaaggactc accgtcctgg atggtcacct
    20101 cagccaatct gaatcacccc ctctcttcct gagctatcaa ttctgggccc gctcgtcaca
    20161 ttcgcggacg aatcggacag gttttctgtt ataactcgta tcaggacgga tacagaagcc
    20221 ggatgcacaa ggtgctggcc attctgctgg ctgggggagc gggtgaacgt ctctaccctc
    20281 tcaccaggaa caccgccaag cctgccgtcc cgttcggcgg tgtctaccgg atcatcgact
    20341 tcacactctc aaactgcatc aattcgaata tccggcgtgt tttcatccta acacagtaca
    20401 aggccctcga acttcaccgg cacgtgcgcg agggctggaa tattctgtcg ccggaactgg
    20461 gagaatacat cgaagttttt ccgccaatga agcggatcca tgaggactgg tacctcggca
    20521 ccgccgacgc cgtgttccag aacacccaca gcatcatcgc cgaggacccg gagtgcacga
    20581 tcattctagc cggagaccac atctacaaaa tggactacgg tgagatgctg gactggcatc
    20641 gtagccaccg tgcggatatc actgtgggaa ccatccaggt gctgcctgag gaagcgcccc
    20701 gtttcggcat tgccgagatc acaccggacc accggattgt cggctttgag gagaaaccgt
    20761 cgcacggcgg cccacaaagg tccgtcttca atcccgagat ggtgagtgcc tcgatggggg
    20821 tctacgtgtt caagacgccc gtgcttttga atgcgctagc ccaggatgct gaagatcctg
    20881 catcctctca cgacttcggc agggacgtga ttccgaggtg gatttcgaag gcccgagtga
    20941 tcgcctacga ctttcacgac ctcaatgaca agacggtgca ctactggcgg gacgtcggga
    21001 cgatcgaagc ctactacgaa gcgaatatgg atctggtggc ggtcataccg caattcaacc
    21061 tgtacgacag taactggccg atccggactc gtctcgaaca acaccccccg gcaaaattcg
    21121 tcttcgcgca ggaaggacgg cggatgggcg tggcgatcga ctcgatcgtc gcgggcggat
    21181 gtgtcatttc cggcggccgc gtgatccgtt ccgtgctatc gcccggggtg cgggtgaaca
    21241 gctattgcga ggtcgagaac tcaattctga tgaaccgggt cgagattgga agatactgcc
    21301 gcatccgcaa cgccatcgtc gaccgcgacg tgaaggtgcc agacggcgcc acgattggct
    21361 ttaacgccga cgaggaccgc gcccgcgggt atcacgtggc cgatggcggc atcgtcgtgg
    21421 ttccttccgc gcaagagact tacgctacca tagcggttta ggcaactttc aaccatcagc
    21481 tttcaaccgg cagccgcgga ttgcgtagtg cgttccctgc gactgccggt gacggctggc
    21541 aacccactta ttttcaagga gcaatgatga ccaagatcgt aaagactgta ggcagggaga
    21601 ttctggattc ccgcgggaat ccgacggttg aggccgacgt ttatctggcc gacggaagca
    21661 tgggccgggc cgcggtaccc tccggcgctt ccactggcga gcacgaggcc gtggaacttc
    21721 gcgacggcga caagtcgcgc tacttgggga agggcacgaa gaacgcagcc cagaacgtga
    21781 acggcgaaat tgccgtagct attcagggca tggatgccgc ccgccaggag gaagtagacc
    21841 gcaagatgat tgccctcgac ggcactccga acaagggacg gctcggtgcg aacgcgttgc
    21901 tggcggtctc catggcagcg gcgcgagctg cggcggcctc gcagcaggtc cccctgtacc
    21961 ggtacctcgg cggcgtatcg gcatcgatcc tgccggtgcc gatgatgaac attctgaatg
    22021 gcggggcaca cgcggataat tccgtcgacg cgcaggaatt catggtgtcg ccagtgggtg
    22081 ctcccacctt tgccgaagcg ctccggatgg gcacggagat cttccacagc ctgaaggcgg
    22141 ttctgaagaa gaagggctac tcgacggcgg tcggcgacga aggcggcttc gcgcccaacc
    22201 tcaagtcgaa cgacgaagcg ctggaggtga ttctcgaggc gatcgagaag gcgggctaca
    22261 agccgggagt ggacgtttgt ctcgcactgg acccggctgc gagtgagttc tacgataccg
    22321 gcaaaggcaa gtacatcttc aagaagtccg acaagagcga gaagagcccc gaacagatgg
    22381 tggagttttg ggcgaactgg gtgcggcagt atccgatcat ctcgatcgag gacggcatgg
    22441 ccgaggacga ctggaaaggc tggaagctgc tgaccgacac gctcggtccg aaaattcagc
    22501 tcgtgggcga tgacctgttc gtgaccaaca cgttgcgcct ggcgcgtgga attcaggaag
    22561 ggatcgcgaa ttcgattctg atcaaggtga accagattgg aacgttgacg gaaacgctcg
    22621 aggcaatgcg catggccgcg gccgcgggct acacctcgat ggtgtcccac cgctcaggag
    22681 agaccgaaga cacattcatc gccgacctgg tggtggccac tggcgccgga cagatcaaga
    22741 cgggctcggc aagccgcacg gaccgtatcg ccaaatacaa ccagttgctc cggattgaag
    22801 aggagcttgg cgcctccgcg aagtacgccg gcctgaaggc cttcaagcgg ttggcgatct
    22861 gaggcgcgcc ggcacagaca aacgcggaga acaactggaa tgtacaaact cgtcttagtt
    22921 cgacacgggg aaagcacctg gaacaaggaa aaccggttca ccggctggac cgatgtggac
    22981 ctttccgaga agggcgtcgg ggaagccaaa gacggcggcc gggtgttgaa ggagcaaggc
    23041 tacaccttcg acatcgccta cacatccgtg ctgaagcgcg ccattcggac gctgtggctg
    23101 gttctcgatg aaatggactt gatgtggatc ccggtggtgc gggattggcg gctgaacgag
    23161 cgccactacg gcgcattgca ggggttgaac aaatccgaga cggcggagaa gttcggcgaa
    23221 gaccaggtca agatctggcg ccgcagctat gacatcccgc ctccgtcgct cgagaagagc
    23281 gatccgcgct ggccggggca cgacgcgaga tacaagagcc tcagcgaaga gcagttgccg
    23341 ctcacggagt gcctgaagga tacggttcag cggttcctac ccatgtggca cgacgtgatc
    23401 gcaccaatcg tgcggtccgg taagaaggtc ctgatcgccg cgcatggcaa cagccttcgt
    23461 gcgctggtga agtacctcga caatgtctcg gaccaagaca tcgtaggcct gaacatccca
    23521 actggcatgc cgctcgtgta cgagctcgat gccgatctca agccgattcg cagctactat
    23581 ctgggcgacc ccgaaaaagt gaaggccgcc atggaagccg tcgccagcca gggcaaggcg
    23641 aagaagtaag ggccgggcag acccggcccg cattggtcta cttcttcccg gtgcggctcc
    23701 actcccggat cgccgcgagc tcgtcggcgg gaatatggag gaactccagg aatttctggt
    23761 gttcctccgg cgccatcttt tcgaactcct tgtgccaccg gtccacgtcc gcttcggtga
    23821 accccgaagc cttcatgatg gaaacccact tctccttatt cagcacgtct gacctccgga
    23881 acgaatgtct gttgaggatt ctgaggatcg cccgctggtg atcgcgaagc tgctcgatct
    23941 cggcatccag ttccgtcagc cgccgtttca ggatggagga agcgtcgcta ttcgggcgat
    24001 cgaagatgtt acggatattg gccagggaaa cacccgcctt acggtagacg caaatctgtc
    24061 gcaggcgagc catctctctg tcgccgtaac gccggcagtt gccgcccgtg cgcccggcag
    24121 gtttgagcac gccgatcgat tcgtaataga gcacggtact gcgggaaagg ccgcaagccc
    24181 gggccaatgt ggaaacggtc aacatcgtcc ttcctccact ctgaacccta cagttataga
    24241 caggtcaagc gcaaagaaac ccgggacagt acactcattt ctagtttccg agtttcgttc
    24301 atcacggctc ggtcagaagc tgaaagcctg gtgagccctt gcccggcgtc gcagagaaga
    24361 cgtcgcgacg agtgatgcca cggagttcct gtatgggaac cagagtagaa tcagcgcccg
    24421 gccagctaat ccggaacagc ggcgagccag tagccggggc gggtctgtat cttgaggtcg
    24481 cggcggacgt tgccggagga tccgaaaatc tgcacctgga tctcgtggaa cccggcctcc
    24541 atcttgttgt tcggcatgta gctgacgagg tactggctgt gcagttcttc gccgatccgc
    24601 gagatcgcgt tctcgagatc cctctcacgc gtaaaggcgt actcgcggcc gccggtgaac
    24661 tcggtgaata cctcagcggg gttgtccacg aagaccgact tcgcctggcg aaagatctcg
    24721 acgaacagcg gaaggaagct cgccgagctg ccttccagac cgcttacctg acgagccgcc
    24781 tccggcgtgg gaggcacgcc agcgggaagc ggacgcgcgc tcggaggaac ggggtccggt
    24841 cggggcggtt gcgtcttcgc cgtgaatgtc gtcatcatcc ggttaatgtt caccgtgtag
    24901 atcaggacgt tggcgagttg cgcgctggtg agagcgtcac gcgcgcggcc ctcgctgccg
    24961 ttgtcccgcg tctcgctgat cagaagaagc acgcggcggt ggtctggagg cctccgggcg
    25021 agcatccgga tggccgtctg cgtcgcgtcg atcattcggt tgctggaagc gccgatcttc
    25081 aggctgttga tcgccttgga aatgttgtcg gagtccgagg tgaagtcctg gacgacgtcg
    25141 acgcggtggg cgaatgagat gatcgccact tcaccttgct cgccaaccac caggccctgc
    25201 agcagcggcg cgatcttctg tattttcggc aggaccgctt gcgtcgccca gctcgtctgt
    25261 atggcgacga ccagcgagat tgggacgaag gaaacgtcga ccttgatgtc ctgcggctta
    25321 gcgttgtcaa ggaggcggaa attctccggt tgcaggccgt gaacgtagcg tccttcgtga
    25381 tcggtcaccg tcaccggcgc cgacaccacg tttacggtgg cgcggaacac ctggtcggaa
    25441 gtctccggct gttgcgccag cagtgcaacc gccagaagcc ccaaacaaac gcatcgaata
    25501 gtggtcatgt tttcaactag ttgacgttag ggaccgtact tcggtttcac cgggacggca
    25561 tgctcagtct cgccgcgcaa caactcgaga atgtgcggca ctaactccac gatagcatcc
    25621 agggactcca ccgcgccgcg cggggagccg ggaagattca cgatgagcgt ctttccccgt
    25681 gtgccggcga tgccccgcga aagaacggcc agcggcgtcg agttccggcc gtgggcgcgc
    25741 atgacctcgc ccagcccggg gatctctttc tcgatgacgc tccgcgtcgc ttcgggcgtc
    25801 acgtcgcgag cggcaacacc ggtgccgccg gtggtgaaaa tggctgaaat gcggccgccg
    25861 tcggtgagag cggctagccg agcggcgatg atttcagcct catcgggaag cacttccagc
    25921 accgcaacct gccacccggc ttctttcagc cggcgcttca cggcaggtcc cgaaaggtca
    25981 ctgcggcttc ccgcgtgggc cgagtcactg atcgtcagga cagccactcg aatcatgctt
    26041 tccttccgcg agccgggcgc cggaagtctc cactcttacc gccggtcttc tcgatcagat
    26101 agacgtcctg gatctcgatc gacttgtcca gagccttggt catgtcgtac accgtgagag
    26161 ccgccaccga cgccgcggtg agcgcttcca tctccactcc agtgggcccg gttgtcgagg
    26221 ccgtggcgac gattcgcacc ccgtccctct ccacgcgcac gtccacgtcg gcatgactga
    26281 ccatcagcgg gtggcaaaga ggaatcagtt cggacgttct cttggcggcg gcgatgcccg
    26341 cgatgcgggc gatctcgagc ggattcccct tcgggttttc cggcagcttc cgcagcaccg
    26401 cgcgccggat acggacaaac gcctgcgcgg acgcggtacg tttggtgtcc ggcttgtcgg
    26461 acacatccac cattcgggcg gcgccggcgt cgtcatagtg cgacagcttc ttcatttgag
    26521 caacactccg atgagctccc cggccgccca atgctcgcgc tcgggatcgg cgacaagaaa
    26581 cacgttggcg cgggccagcg caggaacgtc cgaggatccc tgccagggaa ggggcatcac
    26641 ttcggccccg tccggactca gaagcgccgg cagaaagcgc gtcagaccgg tcttgtgccg
    26701 gaagtccttc gtaagacggg cctgaaagat cggaagcgcg acgccgcttt gcccgccaag
    26761 cagttcgacg gcggccgaag cgaagagacg aaacgtcacc atggtcgaag cgggattccc
    26821 tggaagcccg aagaagaacg ttccgcgcgc gcgcccgaac acgagaggct ggccgggctg
    26881 aatgaggaca cggtcgaagt gaaactccgc gccgaggccg gccaggaccc gttcgacgat
    26941 gtcgtacttt cccgcggaaa ctccgcccga cagcagaagc agatcggcgc ccaggccgcg
    27001 ctcgacgagc ttgcgcgtcg cctcgtgctc gtcacgggct accggaagga tctctggggt
    27061 gccgccggcc acggagacct gaactgccag cgagaaggcg ttggagtttc gcacttggtg
    27121 gtcgagcgcc tcctccccga cgggcacgac ctcgtcgccg gtggcaacga tggcgacgcg
    27181 gggctttgcg tacaccttta tcgcggtctt gccaatggtt gccgccagtg caatggtgct
    27241 gaagtcgagt cgccgtcccg gctcgagaag gcgggcgccg cggcgagctt ccgaggctct
    27301 cggattcaca aactggcccg gcttcgtgtc gccttccaca accacgaagc cgccgtcccg
    27361 ccgcacgtgc tcgaccatca cgacggtgtc ggcgccttcc ggcaggggcg ctccagtcat
    27421 gatctcgact gcctcgcccg gcccgacaac gcccttgtag acctcgcccg cgcggacttc
    27481 accgacaatc ctgaggcgtc cgggaaggtc ggcgtgccac acggcaaaac cgtctcgaat
    27541 ggagcgtgct agcggcgggt agtcgcggtc cgccacgagg tcttccgcga gaacgcggtc
    27601 ggcggcgtcg agcaagcgcg cttgttcgac ccgcggctgc ggacgcgccg cggtgatcat
    27661 cgagcggacg cactcccgtg cctcccgaaa ggaaagagtc ggcacgaagt tcattctagc
    27721 aaccaggaag ttgactcact tgcacactga gagattcgac aggtgctgta atctcggtag
    27781 gggcggcttt atgcgagcgg agaccagcaa cttcatccga aggttcagtc gggtatgcct
    27841 gtcggccggt attgctggag ggatcgcgtt cttggattcc aggttcgctc acgtcaacag
    27901 cacgacagtg gctctgatgc tggtgctggt cgtgctggca gtcgctacca ggtggggact
    27961 tccggaatca atgggcgcct ctgtggccgg gatgctctgt ctcaactact ttttcctgcc
    28021 gcctgtaggc aagtggacaa tcgcggaccc tcagaactgg gtggcgttgt gcgtgttcgt
    28081 gatcaccgcg gtcgtagcga gtcagctttc ggcaagcgcg aaacgccgga ctgccgaggc
    28141 caacagccgc cggcgagaga tggaacgcct ttatgtgttg agcaggtcac tcatgcggat
    28201 ggatacggaa tctccgttcg cgcggcagat cgtgtcccgt attgccgagg tgttcgcgct
    28261 ggaccgtgtg gtcttctatg accgtgacga cgatgcggtc tatggaccgg ggtctgtgga
    28321 tgtggcttcc atggaaggtc cgctgaagga gtcggtggtt ggaggaacct caaccgtgat
    28381 taatgagtgc ggggcgacgg tgtctcccgt taacctcggc agcgtggtga tcggcagcct
    28441 ggcgctgcca acggagagca tgtcgggatc cgcccggcgc gcgatcacgc aacttgcggc
    28501 gatcgccctg gaccgcgccc ggggccagga ggcggcgagt cgcgccgaag ccgcccggct
    28561 gagcgaggaa ctgaaatcca ccatgctcga tgctctcgcc cacgagctca agactccgct
    28621 gacctccatc aaggcggcgg ttaccgcggt tctgggctgc aattcgtctg tgcccacgca
    28681 aacggaactt ctgcaggtca tcgatgagga gaccgatcgt gtcacgtcga tggtcacgga
    28741 agccattcag gtggcccgga tcgaggcggg acagattgag ctgcgaaagg ccccgcactc
    28801 aatggccgcg ctcgtccggc aggtgctttc cctgttctcc ggctgccttg agtcgagaac
    28861 cgtcgaggtg caggtgagcg aatctctccc gccggttgag gccgacgcgg aactggtgcg
    28921 catcgtcatc cggcaacttg tggacaacgc cgccaagtac tcaccccctg cttcgccgat
    28981 ttcgatccgg gcgatgcagc gcggcgagtc gattgtgctg agcgtcgccg accggggtcc
    29041 gggaatctcc gggccggacc gtgaacggat tttcgagaga ttctatcgtg ggcatgccgg
    29101 ccgtggaccg gtctcgggta ccggcatggg gctggcaata gcgagggaaa tcatccgggc
    29161 tcacaacggg gagatcggcg tggaaagcca gttggggcgg ggtagcgagt tccatttctc
    29221 tctgccggtg gcccgtgcgg aagcagaacc atgagcgctc gcattctggt ggtagacgac
    29281 gaaccgcaaa tccggcgagt catgcgtgcc acgctgaccg ctcagggcta caccgtctcc
    29341 gatgccagga gcggtgaaga ggcactggag aaactcggcc aatcgcccca ggatcttgtt
    29401 ctgctggatg tcaacatgcc ggggttgggt gggcttgccg catgccgagc catccgtgcg
    29461 ggattcgacg tgagcatcat catgctcacg gtgcgtaacg ccgagaaaga caaggtggaa
    29521 gcgctggatg caggcgccga cgactacgtc accaagccgt tcggaacacc tgagcttctc
    29581 gccaggattc gcgcggcgct ccgccggtca gcctcgattg cgccggccgg cgcccagccg
    29641 atgaccattg gtgagttcat gatcgatttt cagggacgtc aggtcgtgat cggcgaacgg
    29701 cacacacgcc tgacaccgaa ggaattcgac ctgctgtggc acttcgtgac acatgccaac
    29761 cggacgatca ctcaccgcga tctgctccag gccgtctggg ggctggagta cggcggtgaa
    29821 gtggagtatc tcagagtatt cgtcaatcag ttgagaaaga agattgaacc tgatcccgcc
    29881 aatccgaaat accttctcac aaagccttgg gtaggctacc gcttccagtt gcccggccag
    29941 cgctgattcc cgtttctctt tatgccttct ttacgattct gcctctagcc tgtagtcgtg
    30001 tgaggcgtac gtgcgagtga tcttagcgcg cgctttacaa gggccccgac cagacgggaa
    30061 gggcccagac cgggtgtaga ggaggcttcg gagattcgag tgccgaactc aagccattga
    30121 ggcgggggac ccaagtattt ggggcgcagt ccgaactcgc ggacggggta ctttcgagcc
    30181 ctagcccgtc agctaacctc gcaggcgaat ttgaaagtca ccctcgacga aaaagggtga
    30241 ggaaggaatt tcccgtgaca attcagaatg cgcctgtgac gaccaaggtc gtcgtcgcga
    30301 cgagtgtcat gctgtccttc atttcgttct ggcgtgcagc tgcaatcgtc ctgaacgact
    30361 tggcatcgac agcttactac gtgggtggga tcgccgagac cgcgattggc aaggcggcgc
    30421 catggttcat tctcgccatc atgctcttct cttacgcggt gcgagcgatc tacatcgaaa
    30481 gttgcagtat gtttgtccgc gggggtgtct accgggtcgt ccaggaagcc atgggcggca
    30541 cgctggcgaa gttttctgtg tctgccctga tgttcgacta cgtgctgacc gggccgatca
    30601 gcgccgtcag cgccggcctc tatctggggg gcctgatcaa tgaaacagcg gagtcgtttc
    30661 acctggcgaa cgttcaactt catccaccgc acttcgccgt ggggtttgcg atcgcggtga
    30721 cgctgtactt ttggcgaaaa aacatcattg ggatcccgga gtccagccag aaggcgctgc
    30781 gcatcatgca agtcacgacg gtcatggtgg tgatcctgat cgcatggtgc ctgatcacaa
    30841 ttgcgaagaa gggttaccag ccggtcccgg cgcccgtcgt ctccaatctt catttcagtg
    30901 atggcgcgct gggatggttg aaaggcacgg tccttcccgc cattccgatg atcgcgattc
    30961 tgatcggcct cggccactcg ttgctcgcca tgagcgggga agagagcctg gcgcaggtgt
    31021 atcgcgagat cgctcatccg aagctgaaga acctggagcg agccggcttc gtgatcttcc
    31081 tgtacagctt gctcttcacg tcgctggtct cgttcttcgc ggtcatgatc atcccggatg
    31141 gggagagggg taagtacctg gacaacctga tcggcgggtt gtccgtgttt ctcgtcgggc
    31201 cggcgccgct gcggctgttg tttcacgggt tcgtcgtgct agcgggcata cttgtgctct
    31261 ccggagccgt aaatacgtcg atcatcgggt cgaatggagt tctcaaccgg gtgtccgaag
    31321 atggggtcct tcccgactgg tttcgccacc cgcaccgaag attcgggacc acgcaccggc
    31381 tcatcaatct ggttgtaatc cttcagatca tcaccatcct gatcagccgg ggcgatgtct
    31441 atattctcgg ggaggcttac gcattcggtg tgatgtggag tttctccatg aaggcgcttt
    31501 cagtccttgt gctccgttac aaacgcccgg agggccggga gtggaaggtg ccgttgaatt
    31561 tccgggttgg aaccacggag ataccggtcg ggctcgggct gattaccctg ggattggtgt
    31621 cactcgcggt ggtgaacgtg ttgacgaaga aaacggccac tctatcggga ctagccttca
    31681 ctgccgcttt cttcgtggca tttgaacttt ccgaaaggta ctaccgcgga acggagcggc
    31741 ggcacgccac acaactcgag aagtttcgcg tggaggcgtc ggaggatctg tctgctatcg
    31801 ccgtcggcgc aagaccgggt aacgtcgtcg ttgccgtgcg gaaccccggc catctggatc
    31861 atttgaagaa agtgctcgac aagaccgaca ctcgcaagat cgatatcgtg gtgctgtcgg
    31921 ttcgagtgat cagtcctgcg ggctccggcg agcatgcact gtcgcccggc cagattttcg
    31981 cagacgatga gacgcgcctc ttcagcaagg tcgtgagttt ggcggagcac gcggggaaac
    32041 ccgtcagact cctggctgct cccgcgactg accccaacct ggccctggtc caggttgccg
    32101 agacactcca ggcgacgcga atcgtcgctg ggccatccgc gagaatgggc cttgacgagc
    32161 aagccagact gattggtctc gcgtgggaac ggttgcctga acccaagtcg cctgtcagct
    32221 atgagattgt ctccttggat ggaagatccc agttctacga tctgggaccg catttgccca
    32281 ggctctggcc cgaggacatc gaagtggcgc accgcctttg gctggacctc actgacagtc
    32341 tcggtgcgca actgcaccac cgggacgttg tcggacttgc acttcggcga atggagagcg
    32401 acctgaaatc cggtcgcgcg gcagaagtgg cggaagcact atcccgagaa atcggttccc
    32461 gcccacaagg ttagatttcc ggaccagaat cgctcgcgca agaagtgggt tccgctttgc
    32521 ggacgtctgc cgtttgcgtg aaaatctcta aatgccgcat cggatgacga tggtgcagat
    32581 ggctgcggaa gtcagggcag ggaaggtctc ccccagcgaa ctcgttcagg cacaccttgc
    32641 gcaaatcgaa aagcacaacc ccacactgaa tgcgtttgcg attttacggg cggaggaggt
    32701 gcgagaggcg gccggcaccg ccgaacaggc cctaggacag aagaatctcg gcccgctgca
    32761 cggagttccg gtcactgtca aagacagctt cgacctggcc ggactgccga cgctctgcgg
    32821 cagccggttc cgcctcggac acaaggcagc gagcgattcc accgtggtgt cgcgcctcgg
    32881 caaagccggt gcaatcgttc tcggcaagac gaacaccccg gaatttctga tgagttatga
    32941 aacggacaac catctcaccg gccgcacgaa taatccctgg gatttggagc gcaccccggg
    33001 cggttcaagc ggcggggaag cggcggccat cgcggcattc tgttccgcgg gaggggtcgg
    33061 gagcgatggc ggcggttccg tgcggatacc cgcgcatttt tgcgggatct gcgcgctgaa
    33121 gcctacgccg gggcgcatcc cggcgaccgg tcattttccg gacatctgcc atccgggcgg
    33181 tctgctcggc gtcgccgggc ccatggcgcg gacagcacag gatctccggc tactcttcac
    33241 agttctctcc gggcacgatg cgcaggatcc cttttcggcc ccggttccga ttggaactcc
    33301 ggagttcaag gaactccgcg cgggagtgct ggaacagttc ggcgcagatc ccgtgcaggc
    33361 ggacgtgaga caggcagtct ggcgcgctac ggaaaccctc ggagaactcg gaataccagc
    33421 ggcctccttc gagccgcgtg gcctcgatcg ggcgcaggag ctgtggtggt tctttttcgg
    33481 acggctgaac gccatgctga tccgtcaggt agtgcgggag cgggaagatg aggctcattg
    33541 gacgggcatg gagttggtca cgatggcgga gcaggagcct ccgcccgggg cgatggaaat
    33601 cctcgagaat cttgccgcgc gggaccgcat gcgagccgcg ctgctgcgcc agatggaaga
    33661 aactccgatt ctcttgcttc cggcttgcgg cgtgaccgcg ttccggcacc gggagcggga
    33721 gtacgagacg cccgacgggc cgattgggct cctcgaggcg atggcgccgg cgacgccatg
    33781 gaaccttctc ggattcccgg cggtcgtgat cccgttcggc atgacctacg atgggtctct
    33841 tcccgtgggg attcagattg tcgggcgccc gtgggaagaa gaactgattc tcgaggtcgc
    33901 catccggctg gaggaagcgc gcggcccgtt cctggcgccg ccggggtaca gggactaagt
    33961 aagatggaag tgtatgctgc gtgtcatctt cctcctgttg cttggcgtcc ttgtcgccgc
    34021 ggcggccgga tccccaaaga aagcctttcc ttttgattac gatcagcacg actttccgaa
    34081 tggcctccgg ctgatcaccg tacctaccga ctacccgaac gtcgtggcgc tgtacatcgt
    34141 cgtgcagacc ggttcgcgca atgaggtcga gcccggcaag agcggcttcg cgcacctgtt
    34201 cgagcacgtg atgttccgcg gaaccaagga gtacccgccc gagcgctacg aggccgtgtt
    34261 gaaggccgcc ggggcggcgt cgaacgccta cacgaccgac gaccacaccg cgtatcacac
    34321 caccttctcg aaagaggact tcgagagcat cctcaaaatg gaggcggatc gttttcgcaa
    34381 cttgagctac agcgagggcg tgttcaaaac ggaaagcctc gccgtgctcg gtgagtacaa
    34441 caagaacagc gcggcgccga cctcgaagct ggaagaggtg ctgcgtgaca ccgccttcga
    34501 ccggcacacg tacaagcaca ccaccatggg ctttctgaag gacatccagg acatgcccaa
    34561 catgtaccag tacagcctcc agtttttcga ccgctactac cggcccgaat acacgacgat
    34621 catcgtggcg ggagacgtga agaaagacag cgtgccgtcg ttggtcgagc agtattgggg
    34681 cgggtggaaa cgcggcagct acacggccgc agtgcccaaa gaaccaccgc aggacgctcc
    34741 gaggtcgaac catgtcgcgt ggaatacccc gacgctgcca tggcttacgg ttgcgtttcg
    34801 cggagcggcg tactccgata cggaaaagga tcaggcgacc ctggacctga tcgcgtttct
    34861 tggtttctcg gagagctccg aactctacca gaagctggtc atccaggagc aaaaggtgga
    34921 cgtgattgag ccggacaatt cggaccacgt cgacccgtac ttgttctccg tctggagccg
    34981 ggtgaagaac ggtggagacg tcaactatgt tcgggaccag attctggcga cgttggaaag
    35041 tttcaaggcg cggcccgtgc cgaaagatcg cctggaagcg gtgaagagcc acctccggta
    35101 cagcttcgcg cttgccatgg acaacagcga atcgatcgcg gcgaccctcg cccgctacgt
    35161 cgctctcagg agaacgccgg agacgatcaa caagctgtac gaaatgtacg cgcggatcac
    35221 gcccgaagac gtgcaggaca tcgcgagtaa gtatttctcg gagaactcgc ggacggtggt
    35281 gacgctcgcg ggagggaaag cccaatgagg acgctgctga tacccatgct aatgctggct
    35341 gcaaccggac tttctgcgca gatgcgcgtt gtcgcgcaac ggagcgattc accgctggtg
    35401 acatttcgca ttgtctttct gaccggcgcg gcggcggacc cgtcaggcca agagggcgtt
    35461 gcttcgctcg ccgcgtcgat gctctcgcag ggtggtacgc gagagatgtc gtacaagcag
    35521 atcgtcgacg cgatgtatcc catggcaacg tcggtctcga gccaggttga caaggagatg
    35581 accgtcttct cggccactac gcacgtcgac aacctggacg cgttctacaa gatcttccgg
    35641 gcaatgctgc tcgatcccgg ctggagagcc gacgactttg agcgtttgcg tgacgatgcg
    35701 atcaacttcc tccgcgtgag cctgcgcggc aacaacgacg aagaactcgg caaggaagtt
    35761 ctctacaacg tgatctacaa cgggcatgca tacgggcatc acaacgccgg cgttgtctcg
    35821 gccttgcaga agatgaccgt tgccgacctc cagaccttct acaaggcgaa ttacacgaag
    35881 gacaacctta tcctcggcat cgcaggaggg tattcgcccg cgtttctgga gcgggtgaag
    35941 aaggatttcg aagagctccc cgccaagggc aagcacacgc cgaagtacga cgcgccgaag
    36001 gctgtgcgcg gcatccgggt gaccatgatc gagaaagaca cccggtcggt ggcatattcg
    36061 ttcggctttc cgatcgaggt gaagcgcggt catcctcact ttcctgcgct gctgctggcg
    36121 caggcgtact tcggccagca ccggatgagc ggcggccggc tttacaaccg gctgcgcgaa
    36181 gcgcgcggcc tcaactacgg cgactacgcg tacatcgagt acttcccgag aggcatgttc
    36241 cagttcgaac cggacccgaa cctagcgcgg ccgagccaga tcttccagat ctggattcgt
    36301 ccgttggagc cggcgactgc acacttcgcc ttgcgcctgg caatgtacga gctcgacaag
    36361 tttgtccgcg agggaatctc gaaagacgac ttcgagcggt ggcgcagctt cctctcgaag
    36421 tacgtcaacc tccttacgaa gacgaaggcg gcggaactcg gctacgcaat cgatagccag
    36481 tattatggca ccccggaata cacaacctac atcaggaaca gcctagccaa gctcagtgtg
    36541 gatgacgtca accgcgcgat ccgaaaacac cttcgaacaa cggacctcga cctcgtcatc
    36601 gttgccaagg gctgcgagga gctgaagaag tccttccttg gcggagcacc gtccccgatg
    36661 aactacaatt caccgaagtc cgcggaaatt ctcgaggaag acaaaatcgt cgagaagtgg
    36721 aagattggct ttcaacccga ggcgttccga atcatccccg tggatcaggt gttcgaatag
    36781 cgtctatcga tccgactcga cgaccttcag aacctggttc acaccgacat ctttgaccgt
    36841 ctggtggact ccgcttggcc agatgatctc gatctcatcg atccgcttca tctgccccgt
    36901 tccgaagtgg acaccgaagt ggctggatga gacgtagcca acggcgctcg tcatatggtt
    36961 gaactgcttg ccgatcctga cgaccgcgcc aatgctgtca cggttgctct tcgtgcccgt
    37021 cagcttcagg atcagccacg agttgtctgc cgatgaaacg ttctcccaca actctgcggg
    37081 actctggagc gatgccacga cgacgtcgat ccgcccgtcg ttgttgaagt cggcgaaacc
    37141 gcagccgcgg tgcgcggcgg gagtctggaa gtccggcccg gcgtcgcgag aaccgtccac
    37201 aaaagttccg tcgccacggt tcagaaaaaa gctgtttggt agcttgtacg gcgtcgcctc
    37261 gaacatctcc accgtgtcat tgacgtggga gttggttgtg aagatgtcct tccagccgtc
    37321 gttgtttaga tcgaccaggc cgatgctcca gccgctccga ttatggctga gcggtcccat
    37381 gcggctcgcg aaactcgcat cccggaacgt gccgttgcct cggttctgaa agatcgggaa
    37441 gacctcgccg gcgagagcgg cataggtaat gtcggggaag ccatcgttgt catagtcgcg
    37501 gaagtcggtg cccatcgccg aaacgggcgc gccgttatcc gacaacgccc cgccggcgag
    37561 cagcgcgact tcctcgaacg ttccgtctcc cttgttgtgg aacagaaagt tcgggatctt
    37621 gtcattggtg acgaagacat ccatgaatcc gtcacggtcg taatccgcga tcgacgcgct
    37681 catccctttg ccgatatgtg cggcgatccc ggacttctgg gaaacgtctt caaacgtgcc
    37741 gtcgccgcga ttgcgataga gagtgttcgg agtgccctcg aagaaccgcg ggtggcagta
    37801 ggcgcggact ttggaggccg gatcgccgca gaacaaatcg aatgacgcag tccattggag
    37861 gtagttgacg acgaagagat cgagttttcc gtcgttgtcg tagtcaagcc acgctgcacc
    37921 ctcggaccac cattcgctct tgattcccgc ctttcgcgtg acatcctcga atgtaccgtc
    37981 gccgcggttc cggtagagaa aatgccgccg aactccgccg acgaaaaggt ccgtgtgacc
    38041 gtcgttgtcg tagtccgcag ccgcagcgcc gatcgagtat cccgcgccgg ccacgcccgc
    38101 ctggtccgtt acgtccttga acttcatgtt gcccagattg cggtacaagc ggttgcggta
    38161 cttcggcgag tccttctcga gcgagggaat gctggcgccg ttggtgaaga agatgtcagt
    38221 gaggccgtcg ccgtcatagt cgaacgcggc caatccgccc gccatggtct cgatgagatg
    38281 cttcttcggt gtggggtggt tctccaggac aaagttgatc cctgcgctca ccgcaacgtt
    38341 gcggaacttg acgggtgcgg gagcagcgct ctgtgcgaaa agtgcggaga tcgagatcgc
    38401 acagagagcg gcgacatacg ggatcagtag tgtagtgcgt cgcaagaaat gcaccttacc
    38461 tgccaggttg ttttgtggcg caggccatcg ttttccgtgg cctgcgatgg cggcagacga
    38521 caaaaggcga tcgtctgccc cacgagacat aaggcacaat aactgacgca atacactatc
    38581 gtctctgcct cggcgttttc gatttcacat cacggaagcg ctggatggtt tccgaagcct
    38641 tatctttctg gcccaactgg ctgtagaccc gcgctagcag atacagcggt tccgggtagt
    38701 caggatcgag gaacgtcgaa cgctccagcc atttcacgga cgtctcgtac tgttccagct
    38761 tccaaagagc cttgcctgcc agatagaaat tccgcgccga gttcgcgttc cgttgcgccg
    38821 ccttgactgc ggccgagaat gcctgcttcg cgtccccgtt gtcgatcaaa aggttcgcca
    38881 ggtttgcgta cggccagtcg tattcggggt ggtccgtctc gaccatcttg atggccgtga
    38941 ggaagtggcg gatggccatt tccttctcgc tcttggcctc gtagcacaga ccgaggttgt
    39001 cgtatgcctt gtaagagttc gggttgattt tcaggacgcg ctgaaactgt ccgatcgcgt
    39061 ggtcgatgcg accttcctgg taatagatgc ggcccagcat gtaagcggcg tcttcgtcgt
    39121 tcggatgtcg ggccagaagc tcggccagcg tcttttgcgc cttctcggtc tcaccgagga
    39181 gatattcaca attgccccta agcttaaaag cgtccggttg ctccggttcg atcgtgagcg
    39241 cctcttcagc ctcctgcttc gcctctgtca gttcctgtgc catgaacagg gcgctggcga
    39301 gggcgacatg gccctccgcg gatctcggct cgctctggat atactcgcgc gccacccgga
    39361 tggcctcctg gtgttcgcgc tggtaggaga gcgccctcga caactccagt agcaacccgt
    39421 gctgagctgg acaggctttg taggcctctc gaaggtcgtg cgccgccgtc ggatattgtt
    39481 tgctcgcaag cgcctcgcgt ccggtccgtg acagttcggc gcttcggagt aaatcgcaaa
    39541 cggcaagtcc cgttacctgt gcaaatccgc tcatcggaag taacacgaga acgaacggca
    39601 tcacacgaag cggcggcata acctcttcct tcgagcttac cgcaacagca ccaactcctc
    39661 tttcaccttc gggataatgc cggcgtactg tcgcgacgac gcctttgcga gaaacgtttc
    39721 aaagcacgcg cgcgcccggg cgcgatcacc catcatcttg tagatgagcc cgaggttcaa
    39781 gtgcgcttcg acaaggttcg gatcgattgc cacggccttc tcaaagtacc cgcgcgccgt
    39841 cgccgggtcc tgccgctgga tggcaatcag gccgagcccg ttgtaagcag gcgcgtgctg
    39901 ttcgccggtg gcgaggatcc agttgtagac acggtcggcg tcggccacgc ggccgttttc
    39961 caggtacgcc atggccagat tgttctggct ctcgacgtcg gacgggttga actgagctgc
    40021 cttctcgtaa ttgaaaatcg cctcgctctt gttgccctcg cgaagataga gatctgcgat
    40081 gcgggagtag agtcgtccgt tgcggatgcc tttttccgtc gcactttggt aaagctgcat
    40141 cgcatccttg aaccgccccg ccttctcgta ttccgtgccg aggtggtaat agaccgacgg
    40201 gttcgtcggg tcctccgcca cagcctgctt gagcatctcc atgcgtttcg caggcgggag
    40261 tttcgattcg cccgaaacgg ccagctcgaa gatccgcaaa atgttcacgc gatctttcgg
    40321 gtcgatgccc gtaccggtca gctcgaactg ccgcggggtg aagctggcga gatacccgag
    40381 cgacttcaat tgatcgagcg tccgttgatc caccatcgcc gtgtccactt tctccttgcc
    40441 gtcgccggca atggccttga gctgcgcttc catctcggcc ctctccgtcg ggaaacggtc
    40501 gatcacgttg gccgtctcgt gcgggtcctg ggacagatcg tacaactcgg gcttcggcgc
    40561 gcggatgaac ttccaccgca gcgttcgcac ggcgcgcaac tcgctccagc ccatgttcat
    40621 cttcggatac agtgtctcgg catacgcgtg ttgtgtggcg acgtccttgc ctgctagcgc
    40681 tggcacaagg ctggttccct gaatgccgcc cggaggcttg ccccccatca gatcgaggat
    40741 cgtgggcaac acatcgatgg tccgggcctg ccgtgtaacc cgcatgccgc ccggcactcc
    40801 cgggcccgcc aggatgaacg gaacgcgcag tgtcacgtca tagaggaaaa tcccgtgtgt
    40861 gtattcgccg tgctcgccga ggctttcccc gtggtctgac agaacaacga tgagcgtgtt
    40921 cccggcgttg tctttcttct gcacggcctc aaacagccgt ccgagttcgt ggtccgcgta
    40981 ggcgacctcg ccatcgtacg gccgcccggc gtactgctgc cggaacggcg ccggcggctc
    41041 gtagggcatg tgcggatcga acacatggac ccacatgaag tacggcttgc ccgactgtcc
    41101 gttgagccac tggattgcgc ggtcgaccac cgcgcccgcg ggccgctcgg ggtactctcg
    41161 cgcgtcctgt cctttgccgg gcttgggcat ctcgtcgtca tagacggcga agccctggtt
    41221 gaaaccgaaa agcttcttca gtaccgaagc tccgatgaaa gcggcggtgt cccatccctg
    41281 ttgctggaga atctccgcca gcgtcgtcga tgaggattga agtacgaatc cgcccgtgtt
    41341 acgcactcca tggacgttcg ggttctggcc ggtgaagatg ctcgcatgcg acggcggcgt
    41401 cagtggcgtt tccgcgaccg cattctcaaa caaggcgcca cgctttgcga ggccgtccaa
    41461 caccggcgtt tcgattttcg agtagccgta acagccgagc cggtccgcgc ggagcgtgtc
    41521 gatggtaacg accacgacgt ttagcttgcg cagcggggcg ttcgcctcac cgccggacgc
    41581 aggcttcttc gccgtggatc tacaggagac aagaagcagg gcagcgaaga cgcacagcac
    41641 cctgacactg aacttcgcgc taagcattat ttccagaata tcagccgtgg acgaagcgcg
    41701 gcggaatccc gtctacaata ctccatactt ctcgtaaaca tctcggaggt acgcgccccg
    41761 gcgaagttcc tcgcgggtat ggttctcttt ggtgaccttc tcggtcgcca gccggatcgc
    41821 gtcgggaacg accgccgcgg gtaccactac gacgccgtca aagtccgcga agacgagatc
    41881 acccggcgag accaccactc cgccgcactc gactggcgtg gcgtattcaa cgacgatgcc
    41941 gcgtcccttc gagtccaccg gcttaatgcc ggcggcgaac acagcgaagc cgacctcgtc
    42001 gattttcttc acgtcgcgca ctaggccgtg gatcaccgcg ccccgcgcac cccgtgcgac
    42061 cgcggcggtg gaaagcagtt cgccccacgg agcgttgcgc cgagattctc ccgtcgagac
    42121 gaccacaacc tcgccgggaa gaatgctgtc catcgcttcg atttccttcg cataaggttc
    42181 agccggagta tagtgctggt ccacacaagc gatcgtccgc gcccatccgg cgaagcaatg
    42241 ccgcaccgtt accggccgga ggttctccgt catcgcgtgg ttgcggatgc cgagttcgtc
    42301 caaagcgtcc gcgaccgcgg cggtgtaaag ggtggattca atgcgctcga acaattcggc
    42361 gtcggtctgg tttgccgctc tgaggccctt gtgttccata cgaagctacc gattttcgag
    42421 gttgagcaca ccccatgtca accgaacgtc taaggaaccg caaccgtcag ggagcggaga
    42481 gcggtcaaca caccccaaga aagaaaaagg ccccggtctt gcggccgggg cccgaaagtg
    42541 aagctgttac aagttgctta ccactcgaaa cggccgtcga tctgcaacgt ccgcccacca
    42601 ccgccttgga gcaactgtcc atagccggag tctcctacgg tggtactgac tatgtagcgg
    42661 ttcgtccggt tgagcggatt ggtcatggtg atgccggcct ggaacttgat ccgctcatag
    42721 atcggaaagc tcttggtcat gcggaagttc tccgacattg agtgcggacc gcgagtactc
    42781 ggcaggaccc taggcgcggt tcctacccgc agcggaacac cggcgcccgt tcgcgggctt
    42841 tccgcgaacg ctgccggatt caggtacgga gtcggttcgg accagtcggt cttgcctggc
    42901 gcaccgccca gggttgtgtt ggcgcccgtc gcgtccggac ggatgcccga tgagaaacca
    42961 tccgggatgt ccaatccacc ctgcccgatc gagagcgggt cgcccgaacg gtactgctga
    43021 ttggccgcga actgccagcc gccaaccacg taattagccg gaccgaggtc ccaacgccgg
    43081 cccttgccga acggtacttc gtatacccat gtcaacttga acgaatgcgg gtagttgaag
    43141 ctcgccaccg agcgctccag gccgcggttg aagtaatcct gcaccgtggc gtagtatgcg
    43201 cccggaccgt tgctgtccac ataggtgagc gtctttgacc aggtgtacgc gcccaggaag
    43261 ccgaggcccg tggtcaggtg ccgcgtcacc gtgacctgca tggagttgta cagcgagttg
    43321 gagttatacg ggaaggcctc tccgacgccg aaatactgcg ggaagcggcg catagcctgg
    43381 gaaacggtgt aatcgtctgg gaagtcggcg aacggcttca gcgtcgggtg gtcggccacc
    43441 tgttcacgta acaggtcgcc gtactgcaag agcgaagccg gcaaaccgtt catctcgccg
    43501 aacacgaacg agccccacag ccgggtaccc ttgttgccga tgtaggctaa ctcgagcaca
    43561 gtgttggacg gcaactggta ctgcactgtg aggttccagt tctggacgta acctggacgg
    43621 ttggcgtcgc gggcggtcgt cgtcgaagcg tcgaaattac cggcggacgg attcgtgtcc
    43681 ggcagggtgc ctgcgaatgg ccggaaggtg tccgtcaggt acatggccgg gtcatccaca
    43741 aatccggtcg cgccggtgcc ctgacgtacg ttcacgcccg catcgaaacc gtaccggaag
    43801 ccgccgtagc cccagtcgtt ccggatcggt ggggtgttgg tgatcgcgta tccgccgcgg
    43861 actaccatct tgttgcttgc ctgatacgcg aaaccaaacc gcggtccgat ctccttccag
    43921 taagtgtcgc taggcttcgt accgaagcgc aacgcgcccg gcaggttgcc cgcttccgga
    43981 ttcggcaggc ccatgtcgat gtacgacatc tggttgttaa cctcgtagaa cgacgggatg
    44041 atctcccagc gcaggccgag attcaccgtc agtttcggtg tgaccttcca gtcatccatg
    44101 aagtacaggc tgtgctgggg ctgccggaag tcattaatcc cgccgaagat gttgcggttc
    44161 gcgttgttga cggcgcccag caggaaactg gcaaacgcgt ggccggtctg ggtcgagaag
    44221 gccggcaggt cggtcgagcg gtagctgaac gtgaatgcgc cgccgttctc gacggggtcg
    44281 tccttgtaga agtactgctt gtactcgtag cccatgcgga aggtgtgagc gccccggatc
    44341 atcgtgacgt catcctgacc gatgtagctg ccgttcgttc cggccccata gaagccgacg
    44401 cccatccggc ctaccgagcc gccctgccat tcgcggccgc tgaagctgaa tgtcgggaag
    44461 atcgtgtcgg ggataccgga gataccgaga agcgcgggcc agccggcctg gtaggtgtcg
    44521 ggtatcgctc cgttctggtt gcggaaactg ttaaagccga acgcaaaacg gttcaggatg
    44581 gtcgggtgga ccgtcgaatc cagcgacgca cgcaccatct ttccaggcgt gatctgctct
    44641 tgccaggaac tggtggccgt acccggaatc ggaaggtagc gcgacgatcc gttgttgttc
    44701 cgttttcggt agctctggtt gtagaaaccg ctcactcggt gcttgtcgtt gatgacgtgg
    44761 tcgcccttga ccccgatgag gtgcaggttg aagtacggcg agctggtgct cagacggttc
    44821 ttgttacgga tcatcttgtc gaagtcgggg tcggcgatgc cgactttgtc caggacattc
    44881 cgcgccaccg ggctgatgcg gtttccggga atgacgttgt caaggaacgg gtcgcgaacg
    44941 atctcgccat tcaccgtgcg ggtgctcaac gggtcataga tctgcccgaa aacaaccggc
    45001 cggcccagag catccgttcc gatcgtcgta ccggaatctg cgttgccggt gaacgtgggg
    45061 tcaagaaagc gcgagaagtc gccctgcttt tgatccgtcg tcggcagcgt ggtgaagccg
    45121 ttgaatccca attcgttgcg cgtgtccttc tcgaagttcg tgaagaagaa ggtcttattc
    45181 cggccgtcgt aaatcttcgg aatataaacc ggaccgccga ccgagtaacc gtaattgtgc
    45241 tcccggtggc gggacttctt cgtgatgccc tgggtcttct ggctcaggtt gaacgcgtta
    45301 aagtactcgt tctggccgta gtagaacgcc gacccgtgca gatcgttggt gccggacttg
    45361 atgctgaagt tcgccaccgc cgtctggccg ccgttatact gcgcgccgat cgcgccggtc
    45421 tggagcttga attcgctgat cgcttccgcc gacgggctga actcgttgtt attgccgccc
    45481 gaaaggtctg agcgtccgac ggccatgccc tctatcagaa tctcgtggga gtactgctgt
    45541 ccgccgttga tcgaccccag gaacgttccg ccggtcgtgc cgggcagggc gtcgaaaatg
    45601 aactgctgga tctggcgctg tccgtcgtcg acgaagatcg gccagctctt gtactcttcc
    45661 gtcgaaatgt aacggccgat ttccgcgctt ccggcttcga gcagcggcgg cgcggcgctc
    45721 accgtcacct gctcggtgac cgcgccaagt tccaagttga tgttgaccga tagcgtctgg
    45781 ccgacgcgga ggataacgcc ttcccgcgtc gaggtacgga agccaggcgc gccggccttg
    45841 attgtatacg ttccggcagg caggtacgga agggtgtacg cgccggccga cgtggaggtg
    45901 gctgtgttct cgactgcagt tgcgacgttc gtcgcaacca ccgtcgcacc cggaaccacc
    45961 gcgcctgaag catcgagaac ggtgcccgtc agggttccga ctgaaccctg cccccaggca
    46021 accgacaacg acgccagcag gcacaaaact agctttagat agcttttgtg cattcgtatc
    46081 ctcttttttt tgtctgtctc aaacagcctg taaccgcttg catcgacaat atttacctca
    46141 ggcaacatct cccctgcctg aggcagaccc ctagctcgcc ttttgattgg cgttaagccg
    46201 aattatagtg cgaatatact accgtgtcaa ggcttgcccc cggtgtttac ctcgttaaat
    46261 cagcttgttg caaatgttgc atgtttctgg gagacaggcg atttctaagt gtggctgagg
    46321 cggcggactc agtcccatgt cacccgcgtc tgcttctcga ccttcgccgg gtccaggtca
    46381 atgccgaatc ccggatgctc cggcagcgtg atcttgcccg ccgtgggacg caacggctcc
    46441 ttctcgaagt ggtggtacgg ctccatcttc gtgagaaggt actccacgag cgggcacgtg
    46501 atcggcggct ggctggccac cacatgcagc gccgcgtgca agctgtgccc gtggggtatc
    46561 acctggacgt cgtaaagcga cgcgaccgtg cagatcttca ccagttcgct gatccctccg
    46621 caccattccg ggtcggcctg caccacgttg agcgctcccg ctttcaggta gtcctgcacc
    46681 tcccaccggt tgtagatgtg ctcgccggaa gcgaccggga tggacgtcgc gcggcgaagc
    46741 tgggcgaagc tctcgatctt gtcggtgggg aagcattcct cgatccatcg cggccggtat
    46801 tgttcggcct gcttcgccca gcggattgcg taggtcacgt cccagcccat gaacgcgtcg
    46861 aacatcagtt ccacttcgtc tcccaccgct tcgcgaaggt tgcgcacgag ggcgacgttt
    46921 ttcgccagcc cgtccgtgcc gtcgcccggt ccgtacgcga agaaccactt ctggtatcgg
    46981 aagccttcgc tcttcagagc tgccgcgcgc cgcctagccg cctccggttc gacagacatg
    47041 ctcgcgcagc ttccgtaagc ctctacggcc ggacgcgtcg gaccgccgag cagcctgtag
    47101 acgggtactc cgtagtagcg tgcgcgcagg tcccatagcg tgttgtcgac ggcgctgatc
    47161 gccatcatga agtgaccccg gcgcgcgtgc cggtcggagc ggaacatctg gtcccagagc
    47221 ttctcgccgg ccagcggatc cttcccgatc acgaacggct tgagttgctg ctcgactacg
    47281 atcgccgctt ccttgtcgat cggaccatag acaccctcta gtcccgcgtc ggtcttgatc
    47341 ttcaggtaca gggcgctcgt cgcgacccgc gccggtttgc cctcggccgg ttcatggtac
    47401 ggtttcggcc ggtgctcctc gtagatgtgg agcgggttgg aatgctgctg ccggttgatg
    47461 ccgacgatcg tgtcgcggtg gccctccaga cgccacattt cgacggcggt gatctttagt
    47521 ttgtcagacg gagagcgctg gccgaacgaa ggaacggaag ataatggcgc cccggacgcg
    47581 gctgccaggg ccgtaacgaa ttcgcgtctg cgcatgcggg aattctatga ccgcgacacg
    47641 cgagggagca agccccgcgc cgctactcgg cgtcctcttc gaggatggct cccttgtgct
    47701 tcggccgctt gcgcttggtc ttgggttcga tgattctggc ggacggaacc gtaccaacgc
    47761 gctcgcgagc gaggcgccgg acctccttct tccggtcgaa tttcagcttc ttccgcatat
    47821 cagacaaacc cagccgctcg attcgatccg gtcgtgacac gcaaagccga agctcgcgaa
    47881 caggtgctcg acgcgctcgg cctcgtagcg ctcgaacccg gtaacgatgg cctcaccgcc
    47941 gggcgcaagg acgcgtgcca tctcgccagc aagttgcctg atggccgccg cgttaaggtt
    48001 cgccacgagg agatccaccg cgccgctcct cacggaccgg gcggagccgg cgaacagtga
    48061 cacgggaact cccgcctcgc gcaggtacga agcggctgct tcgaccgcag ccgtctcgat
    48121 gtcgcaggca atcacgctgt tcgctcccat cagaatcgcg cccagcgcga ggataccgga
    48181 gccggtgccg aggtcgaaaa cgcgccggcc gggacggacg cggcgctcca gagcttcgag
    48241 cgcaagctgc gtggccggat gaaagcccgt ccccggcgct gagcccggcg gcatcacgag
    48301 gcgcagccgt cccgccggcg tcggatcatt acgccaggag gggacgagga agaagcgttc
    48361 gccaacgagc tccggttccc actgcgcctg cgaatacgcg acccaatcgc ggtcctcgca
    48421 cgcctcgacg gtgacgttcg aggcggaaat tctcccggcc accgcggccg ccgtggccat
    48481 gtcttcgaaa aacgcttcca gccggcagcc gccctcgaac aggtcgtgct cgacgatgcc
    48541 ctcggtgccg gcgtcggaga gctcggcaac gagcccgtcc ctctcttcgg cccggcactc
    48601 caccgtcaga cggaacatcg gtctcaccgc cccaaaatgg gttgcaactc gatctttcgg
    48661 aagaacacct ccgcgccctc ggactggaac aggatcttgc cccgtgtcac cgaagacttc
    48721 gatccttcgt tcaccagctt gccgttcacc aggaagcgga agttgtcgcc gtcagcccaa
    48781 agctcgagct tgttccactg gccgtgtggt ttctccgggt cgccgtttgg atcgcggtaa
    48841 ccggcgacgt cttcccattt gcccttgccg aagcggtcga agcggccgcg tcgtttcgtc
    48901 tcgcctttca ccgtaaccgc ggtgtctccc acgaggatca ggtcgcccgt gccgccctcg
    48961 atcatctgga attcgatcga ctgcggccat agcttgtcct cgccgccggc gtgatacagg
    49021 atgccgctgt cgcgcgcgtt tccttcgcga gggggaaagg ttttcgagcc ccatcggaat
    49081 tctacgagca ggtaatagtc ctcgtactcc ttttccgtga tgaagtaccc gtactcctcg
    49141 ccggatgcgc ggatctggcc gttttcgacg cgaaacacgc ctttcgggtc gttattcttg
    49201 ccgtatttct cgatgtaggt gtagaacccg tccaggctct tgccgttgaa cagcttctct
    49261 tttggcccga gtctcggacc ggcggctagc acgagcgtgg cgcaagtgag cagggaaaga
    49321 gccgtcttga tcatccctgt attctcgcgc ggatctccgt cttccggatc tttccactcc
    49381 cggtctttgg cagcggcgtg tccgtgaact cgatccgccg cggcattttg aatttcgcga
    49441 tgcggccgcg gaggagttcc aggatgtccc ccgccgtgat cgtcgcgccg ggccgcggga
    49501 cgatgacggc aacgggaact tctccccact tctcgtccgg agcggcgaca acggcgcatt
    49561 cggcgacatc cgggtgcgcg gtgatcgcgc gttcgatctc gatcggtgca atgttttcgc
    49621 cgccgctgac gatgatgtct ttcttccggt cgacgatatg gatccgtccc tcgtcgtccc
    49681 agacggccat gtcgccggtg tacagccatc cgtcgcggag agccgcttcg gttcccaccg
    49741 gatcgttgta gtaccccgcc atgacgtgat cgcttcggat gagaacctcc ccggccgttg
    49801 tcatgtcgcg gggcacgtcg cttcctccgg aatccgctac ccgcaattcg acgccggcga
    49861 tcggcgtgcc ggccatcgcc tggatgcggt atcgctccga gtcgggagca gtcgtgttgt
    49921 ccggtctgcc aaccgtcacc accggaccgg cttccgtcaa cccgtagctg gcgaggcact
    49981 cgcattggaa tgcctgttcc atgcgctcga tgagttcggg cgacgacgcg gccccgccga
    50041 tcagaatgtg cttcatactc gacacgtcga atcgtctgcg tccgggagcg gcgagcaacg
    50101 cgccggccat ggtgggcacc aggcacatgt aggtgccccg gtgccgctcg atcagaacga
    50161 agaccgtctc cgcgtcgaac cgccgcacca tgacctgcgt ggctccgagc aaagcggcgg
    50221 cttgaggata cccccacccg ttggcgtgaa aaagcgggat ggtctgcagg acgacggtgc
    50281 gatcgccttc gggcatcagg agagccacag actttccgtg gaggtagaga gcacggtggg
    50341 tgagcagaac ccccttcggc gtgccggtgc tgccgctggt gtagaagagt tcggcgggcg
    50401 agttctcgtc gatccccgtc caatcgattc gccgggcgct accggcggcg agaagctcct
    50461 cgtaacctga gtccccgtcg agcgggaggg cacacttcag cggcggacag accgcgcgcg
    50521 cctgctcggc caccggcgcg aactccccgt catagagcag cacgctggcg ccggaatgct
    50581 caagaagctg cgacagttcg gatgcggcca gtcgcacatt cagcggcaga aggatcgctc
    50641 ccaccatcgg cacgccgaag tacgcctcga gcagttggtg acggttgaaa cagaggcagg
    50701 cgacgcggtc gcccggcttg accccaaccg cacctaggcc cgacgcgagc cgctcacaac
    50761 gctccgcgaa ctccgcatag gtgaacgtcc ggtcgccgga aatgacggcg gctttgcgcg
    50821 cgtaaagatc tgctgcccgg tagaggaacc ggatcggcgt cagtggcagc accatgagtg
    50881 tccggtcagc gtcccgcggc gcctcccgcc tgcgggacga actcctcgaa aacagcggag
    50941 cggagaagcg agggcgggac cagaccctgc gccagtacga agtcgtggaa gcgcttggcg
    51001 tcaaacttgc cggccatcgc tttctccgcc tcctgccgca gttccatcag cttcgtgtag
    51061 ccatagtaat aggacggggc ctggccggga gcgcgaaacg tgtaccgctc cacttcctgg
    51121 gtggccatcg cgtccgacat cacgacgtcc tccaacagaa cacgcttggc ctcttcgggt
    51181 gtgattcggc tgagctggag ctcgggatcg agaaacgcgc gcgcggcgcg cagcagtcgg
    51241 tgctggagcg cgatcagttg cccctcggcc ggcagaaacg gcttgatcac agcctcggcg
    51301 tagagccccc agccctcgac gttggtactg ttgaaggcga acaccgcgcg cgccgtcgac
    51361 acaccctgtt cgacgattga ggcgaattgc atctcgtggc ctgggcgtgc ttcgtgggcg
    51421 gtcagggtcc acgacgcagc ctcgaaggtg aaatcgtcga aggatttcat ttttcccgat
    51481 gtgtccggca cgttcagcgg
//
LOCUS       DATIFQ010000502         2401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1703070, whole genome shotgun sequence.
ACCESSION   DATIFQ010000502 DATIFQ010000000
VERSION     DATIFQ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703070"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2401
                     /locus_tag="VLE22_25340"
     CDS             <1..>2401
                     /locus_tag="VLE22_25340"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HSB17798.1"
                     /translation="FYTASPRDLGPHDAARGLSDASAGDRLIRTLHGRQQLRGVPFEL
                     GPSDPAQKAWIVLSRRAAPGLAPRAELAVGAMANYLCLAQFCNWDEAERPAPGADPIE
                     RVGEVLAEVGLVYEDGSRHSVPIRRRFEVNALNYAWGHLCFAAEPHRAPVPRKLSDPL
                     PNGFYWGDLQLAVWDPNYPSDPSLDWQGTLWLSAIENPSPEKQIRSLILEARSEDWLA
                     VAGVTLCRTGGHPLRYERLSLYRFTLPPGEDGPWEVSVDTGVVARSYRLPPFDAAAWL
                     SSPVRGLSEPRPDERTDYLYAEVAATGGATLTLANTKTGGRWDFDVREITPGGEVEGR
                     ERGTKVQLLEREKVWLRGAVTDATNGKPTPVRIAFRSRDGRYLPPYGHRTEINSAWFQ
                     DYGADLKWGDTSFCYVDGTFQIELPVGEVYVEITKGFEYEPVRKRLQIDSKQRELSLE
                     ISRFADLRRQNWVTADTHVHFLSPTTAILEAQAEGLNLINVLAAQWGDLFTNVGDISY
                     GPLASKDGDTLIWVGTENRQHILGHIGLLGNHDPVYPMGASGPEESYIGTPVLTSLAE
                     WADACREREGLVVAVHFPFPTAELAADIVLGKIDAVELWPVNITEHFNNLRFLDWYRC
                     LNCGYRLPAVAGTDKMGAWIPAGAYRAYARLGENEFNFANWAKAVREGNTFMSSGPLL
                     FFEADGRSPGGEITFRSGGGSVEVRAEVKSTVPVHRLDIVVNGKVVASREAASGVREL
                     TLRETLKVSGPGWIAARCASKHLSAGTRIAAHTSPVYLVVPGEEAFSAPAAAYLLTLI
                     DG"
BASE COUNT          406 a          778 c          794 g          423 t
ORIGIN      
        1 gcttctatac tgcgtcgccg cgcgacctcg gtcctcacga cgcagcccgc gggctctcgg
       61 atgcatcggc cggcgaccgt ctgatccgca cgcttcacgg acgccaacag cttcgcggcg
      121 tcccgtttga gctcggtccg agcgaccccg cgcagaaagc ctggatcgtc cttagccgtc
      181 gtgctgcccc gggcttggcg ccgcgcgccg agctggccgt cggcgcaatg gcaaattatc
      241 tgtgtctcgc gcagttctgc aactgggacg aagccgagcg gcccgcaccg ggcgccgatc
      301 cgatcgagcg cgtcggcgag gtgctggccg aggtgggcct tgtctacgaa gatggcagtc
      361 ggcattcggt ccccatccgc cggcgcttcg aggtgaacgc cctcaactat gcttggggac
      421 acctctgttt tgccgccgag ccgcatcgcg cgcccgttcc acgtaagctg agcgacccgc
      481 ttccgaacgg cttctattgg ggcgacctcc agctcgcggt ctgggacccg aactacccca
      541 gcgatccttc gctcgactgg cagggaacgc tgtggctgtc cgccatcgaa aacccgtcgc
      601 cggaaaagca gatccgcagc ctcatcctgg aagcccggtc cgaggattgg ctcgctgtcg
      661 ccggggtgac cctgtgccgc acgggcgggc atcctttgcg gtacgagcgg ctcagcctct
      721 accggttcac gctgcctcca ggggaggacg gcccgtggga ggtttccgtg gacaccggcg
      781 tcgtcgcgcg cagctaccgg cttccgccgt tcgacgccgc cgcgtggctg tcttcgccgg
      841 tgcgagggtt gagcgagccg cggcccgacg agcgcacgga ctatctatac gccgaggtgg
      901 cggccaccgg cggagccact ctcacactgg cgaacacgaa aaccggcggc cgttgggatt
      961 tcgacgttcg tgaaatcact ccgggcgggg aagtcgaagg gcgcgaacgc ggcacgaaag
     1021 tgcagttact ggagcgcgag aaggtctggc tgcgcggcgc cgtcacggac gctaccaacg
     1081 gaaagcctac ccccgtgcgt atcgcgttcc gatctcgcga cggccgatac cttccgccgt
     1141 acgggcaccg caccgagatc aactccgcct ggtttcagga ttacggcgcg gacctcaagt
     1201 ggggagacac gtccttttgc tatgtcgatg gaacgttcca gatcgagctg cccgtgggcg
     1261 aggtctatgt tgagatcacc aagggcttcg aatacgagcc ggtcaggaaa cgcttgcaga
     1321 tcgactcgaa acagcgcgag ttaagtctcg agatctctcg cttcgccgat ctgcggcggc
     1381 agaattgggt caccgccgac acgcacgtcc acttcctatc gcccacaacg gccatcctcg
     1441 aagcccaagc cgaagggctc aatctgatca atgtcctggc ggcgcaatgg ggcgacctgt
     1501 tcacgaatgt cggcgacatt tcgtacggcc cgctcgcctc caaagacggc gacacgctga
     1561 tctgggtagg gacggagaac cggcagcaca tcttggggca catcggcctg ctcggcaacc
     1621 atgacccggt ttatccgatg ggggcgagcg gtcccgagga gagctacatt gggacgccgg
     1681 tcctgacaag cctcgccgag tgggcggacg cttgccgcga gcgggagggg ctggtggtcg
     1741 cggtgcactt tccgtttccc accgccgaac ttgccgccga catcgtgctc ggcaagatcg
     1801 acgccgtcga actgtggccg gtgaacatca cggaacactt caacaatctt cgctttcttg
     1861 attggtatcg ctgcctgaac tgtgggtacc ggctacccgc cgtcgccggc accgacaaga
     1921 tgggcgcttg gattcctgcc ggggcgtacc gggcttacgc ccggctcggc gagaacgagt
     1981 tcaacttcgc caactgggcc aaggcggtgc gtgagggcaa cacgttcatg agctccggac
     2041 cgttgctctt cttcgaggcg gacggccgtt cgcccggagg agagatcacg tttcgaagcg
     2101 gtgggggaag cgtcgaggta cgcgccgagg tgaagagcac ggtgccggtt caccggctcg
     2161 acatcgtggt caacgggaag gttgtggcat cgagggaagc ggcctccggt gtgcgcgaac
     2221 tgaccctgcg ggaaacgctg aaggtgtccg gtcctggctg gatcgcggcg aggtgcgcat
     2281 cgaagcacct ctcggccgga acccgcatcg ccgcgcatac gtcgccggtg tatctggtgg
     2341 tccccggcga ggaagccttt tccgcgcccg cggcggcgta cctgttgacg ctgatcgacg
     2401 g
//
LOCUS       DATIFQ010000503         5635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1514040, whole genome shotgun sequence.
ACCESSION   DATIFQ010000503 DATIFQ010000000
VERSION     DATIFQ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514040"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VLE22_25345"
     CDS             <1..534
                     /locus_tag="VLE22_25345"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17799.1"
                     /translation="LEALVAAGCAEITVDEPSMSCYAWREDTSRFVDIFNRTVEPVFG
                     KCRLSTHLCFGNYKGRAVGPRRYARMFPAFLNFKVDEIHLEMASREFAEIEIVAEIAR
                     SKDVAVGIIDVKSYYIETVEDVAARVRRCLEYAPAARLSFTPDCGLSQTARWAARQKL
                     ANMVAGVRVVRRELGLE"
     gene            531..1466
                     /locus_tag="VLE22_25350"
     CDS             531..1466
                     /locus_tag="VLE22_25350"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSB17800.1"
                     /translation="MTLLGIDIGGTRIKAGRVDSSGHIEASSGVPTPASLPALREALI
                     ALVRELSEKSPPSAVGFACKGIINPDTTRVESLPGTVSFLEGHVLKDLIANVLPAGAP
                     VFADNDARAAMAGELAWGAARGHANTLMLTLGTGVGGAVVVGGKLLRGAWGAAGHLGH
                     VTVDPDGSPCICGSHGCIETVFSARAIEAEAFAAAHRGCDTVLTAELERGTLTCEAVF
                     RAAREGDEIARRIIARATKALGAAIAGLFHAFDPEIMILGGQIAEAGESLFDALREET
                     AWRTQRLLRRRIPIVPAQLGDRAGIVGAAALTLGM"
     gene            complement(1471..3264)
                     /locus_tag="VLE22_25355"
     CDS             complement(1471..3264)
                     /locus_tag="VLE22_25355"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSB17801.1"
                     /translation="MKKVILFLFTVSVLCAQEIPRSEYPQPQFQREQWLTLNGRWEFE
                     FDDANVGFEQNWASGAKKFSRTILVPYCFESKLSGIADTSFHPWVWYRRSFSVPDAWK
                     GKRVLLNFGAVDYRSIVWVNGQRAASHTGGHVPFSIDVTPLLKAGPNVLTVRAEDPPT
                     DRTVPRGKQYWEPQSEGIFYTRTTGIWQPVWLEAAGESYFEKVRITPSNDGTVRFDAA
                     IARYQPGAEFHAVIRSGKDAVSSGMVRADERRVALALLVNEPKLWSVNAPNLYEVTFE
                     LRRGGAVVDRVQSYFGFRSVSTGNGRVLINGRPTYLKMLLDQGYWPESTLTPPTDEAI
                     QYDIRIAKEMGFNGVRKHQKLEDPRFLYWADKMGFYVSDEMANAFEFDEDYVSRFTAE
                     WIQVVDRDYNHPSVIMWVPINESWGVPNLLDSRQQNHLKALYTLTRSLDATRPVVDND
                     GWEHVDTTDLFAMHDYARSGDLLYEKYKDLGKPGFRMPNVSRATLVPGYEYNGTPILL
                     SEFGGIAYIPPGAQVPADAWGYSGVEKTPEAALARLRGLYEAIAKIPAFAGVCYTQLT
                     DVEQEINGMLTYDRKPKFDVKAVREINELLK"
     gene            3326..4000
                     /locus_tag="VLE22_25360"
     CDS             3326..4000
                     /locus_tag="VLE22_25360"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSB17802.1"
                     /translation="MRVIPLLLAASLAKLAAQSQDPYEAARVAMETSIESQRAAIRKQ
                     VRSAQAPGHSFFSVPWAEESDAPRLAVPVAAQECDPVPPEQIGPLIDEVSKREGLTPD
                     LLRAVIEKESDFRPCAVSAKGARGLMQLMPATAGQLGVTDPFDPRQNVGAGARFLRQL
                     LDRYSGDLSLALGAYNAGPGRVDFFGGVPAIAETLKYVFDIQEKLKPAELLAPSADSM
                     LAPPQP"
     gene            complement(3939..5027)
                     /locus_tag="VLE22_25365"
     CDS             complement(3939..5027)
                     /locus_tag="VLE22_25365"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HSB17803.1"
                     /translation="MPSPTRSRLLFADRLRGLALVVMIETHVFNAMLSEGLRQTGWFR
                     VLNFVNGLVAPSFLFISGFVFLVSAERKVPEFRTFGRTFWIQLRRIGVVWLIAYVLHV
                     PPAVFGKPWSSLTRKDWLPLIQVDVLHCICVTWLFLLAGLMLLRSPTIRRWWLLSGAI
                     MATLAAPWMWSIRFDEYLPAPLAAYFNDGLNSLFPVFPWSGFMLAGALCASSFLAARE
                     REMERTFMLRLSGLAAVFVALGLALPELPLAPGRTEIPWQADPRTFFLRLGLVFLLLC
                     VCWLVDRRRQSGKSWLFLVSRESLFVYVAHLTVLFAPVFGGRSLADAAGKSMGPGWCA
                     LASAALLSVMVAAARAWNRRKARGAQPA"
     gene            complement(5068..>5635)
                     /locus_tag="VLE22_25370"
     CDS             complement(5068..>5635)
                     /locus_tag="VLE22_25370"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB17804.1"
                     /translation="ALVLAVAPLAAGSLTEEERDFAMSSLHASRKLFLDAIAGLSEAQ
                     WKFKPAPDRWSIAEVAEHITLTEGVLYNLVTDKVMKTEPVTDRAPVKHEQDEELLARM
                     RDRSKKAKASEEMSPKGKFPTPAAAAEAFKQERDRNINYIQTTQDNLRSHVQGKMDAY
                     QYFILIAGHAERHVAQMNEVKADPKYPR"
BASE COUNT         1037 a         1818 c         1734 g         1046 t
ORIGIN      
        1 ctcgaagccc tggttgcggc cggttgtgcg gagatcacgg tcgatgagcc ctcgatgagc
       61 tgctatgcct ggcgcgagga cacgagccgc ttcgtcgaca tcttcaatcg cacggtggag
      121 ccggtcttcg ggaaatgccg cttatccaca catctttgct tcggaaacta taaggggagg
      181 gccgttggcc cccgccgcta cgcgcggatg tttcccgcct ttctcaattt caaggtggat
      241 gagatccacc ttgagatggc cagccgcgag ttcgccgaga tcgagatcgt cgccgaaatc
      301 gcccgctcga aggacgtggc tgtcggcatc atcgacgtca agagctacta catcgagaca
      361 gtggaggacg tcgccgcccg cgttcgccgc tgcctggaat acgcgcccgc cgcccggctg
      421 tcctttacgc cggattgcgg tctcagccag accgcccgat gggccgctcg tcagaaactg
      481 gccaacatgg tggcgggtgt ccgcgtcgtg cgccgggaac tggggctcga atgacactac
      541 ttggcataga catcggcggc acgagaatca aggcgggacg cgtcgattcg tcggggcaca
      601 tcgaggcatc cagcggcgtc cccactccgg cgagcctgcc ggcgctccgt gaggcgctga
      661 tagcgctggt acgcgagctt tccgaaaagt cgcctccctc cgccgtcggc ttcgcctgca
      721 aaggcatcat caatccggac accacccgcg tggagtcctt gccgggaacg gtgtcgttcc
      781 tcgaaggtca cgttctcaag gacctgattg cgaacgtgct cccggccggc gctcccgtat
      841 tcgccgataa cgacgcccgt gcggccatgg caggcgaact cgcctggggc gccgcccgcg
      901 ggcatgccaa cacgctcatg ctgacactcg gtaccggtgt cggcggcgcg gttgtggtgg
      961 gcgggaagct actccgtgga gcgtggggcg cggccggcca cctcggccac gttaccgtgg
     1021 acccggacgg ctcaccctgt atctgcggca gccacggctg catcgagacc gtcttttccg
     1081 cgcgcgccat cgaagccgag gcgtttgccg cggcgcatcg cggatgcgac acggtgctca
     1141 ccgccgagct cgagcgcgga acacttacct gcgaggccgt ctttcgcgcc gcgcgggagg
     1201 gcgacgagat cgcccggcga atcatcgccc gggccacgaa ggcgctcgga gcggcgattg
     1261 ccggtctctt ccatgcattc gatcccgaaa tcatgatcct cggcggccag atcgcggagg
     1321 cgggcgagtc cctcttcgac gcgcttcgcg aggagacggc gtggcgcacg caacggctcc
     1381 ttcgcaggcg gattccgatc gttcccgcgc aactcggcga tcgggcaggc attgtagggg
     1441 ccgccgccct gacgctcggg atgtaggttt ctatttcagc agttcgttga tctcccggac
     1501 ggccttcaca tcgaacttcg gcttgcggtc ataggtcagc atgccgttaa tttcctgctc
     1561 cacatcggtc agttgcgtgt agcacacgcc ggcaaaggcg gggatcttcg cgatggcctc
     1621 atagagcccc cgcagacggg ccagcgccgc ttcgggcgtc ttctccacgc cggagtagcc
     1681 ccaggcgtca gccggtacct gggcgcccgg cgggatgtag gcgataccgc cgaactccga
     1741 cagcaggatc ggagtgccgt tgtactcgta gccggggacc agggtcgcac ggctgacgtt
     1801 cggcatccgg aaacccggct ttccgaggtc cttgtacttc tcgtagagga ggtcgccgct
     1861 ccgggcgtag tcgtgcattg caaagaggtc cgtcgtatcg acgtgctccc aaccgtcgtt
     1921 gtccaccacc gggcgcgtgg cgtcgagcga tctggtcagg gtatagaggg ccttgaggtg
     1981 attctgctgc cgggaatcga gcaggttggg cacgccccag ctttcgttga tcggcaccca
     2041 catgatcacc gacggatggt tgtagtcgcg atcgaccact tgaatccact cggcggtgaa
     2101 ccggctcacg taatcttcgt cgaactcgaa cgcgttcgcc atctcgtcgg agacatagaa
     2161 gcccatcttg tccgcccaat agagaaacct tgggtcttcg agcttctggt gcttcctcac
     2221 gccgttgaat cccatctcct tggcgatgcg gatgtcatac tggatcgctt cgtctgtcgg
     2281 tggcgtgagc gtgctttcgg gccagtagcc ctggtcgaga agcatcttca gatacgtcgg
     2341 acgtccgttg atcaggaccc ggccgtttcc ggtcgaaacc gagcgaaacc caaagtacga
     2401 ttgaacccga tccaccaccg cgccgccgcg gcgcaattca aaggtcacct cataaaggtt
     2461 tggcgcattc accgaccaga gcttgggctc attcacaagc agcgccagtg cgaccctccg
     2521 ctcgtccgcg cgaaccatgc ctgagctcac ggcgtctttg cctgagcgga tgactgcgtg
     2581 aaactcggcg cctggctggt agcgagcgat cgcggcgtcg aatcgcacgg taccgtcgtt
     2641 cgacggcgtg atccgaacct tttcgaagta gctttcgccc gccgcttcga gccagacggg
     2701 ctgccagatg ccggtcgtcc gggtgtagaa gatcccctcc gattgcggtt cccaatactg
     2761 ttttccgcga ggcacggtcc gatcggttgg cgggtcctcc gccctcacgg tcaacacgtt
     2821 cggaccggct ttcaggagcg gtgtgacgtc aatcgagaag ggaacgtgcc cgccggtgtg
     2881 gctggcggcg cgctgtccgt ttacccacac gatcgagcgg tagtccactg cgccgaagtt
     2941 caaaagcacc cgcttcccct tccaggcgtc gggcacgctg aagctgcggc ggtaccaaac
     3001 ccaggggtgg aacgacgtgt ccgcgatgcc gcttagcttg ctctcaaagc agtacggaac
     3061 caggatcgtg cggctgaact tcttcgctcc cgaagcccag ttctgctcga accccacgtt
     3121 ggcgtcgtca aattcaaact cccaccggcc gttgagtgtc agccactgct cgcgctggaa
     3181 ctgcggctgc ggatactcgg aacgcggaat ctcctgtgcg cacaacacgg agacggtaaa
     3241 taagaacagg attacttttt tcatggtaaa caccatgcat cttatctcaa gtccttgcgg
     3301 ccatctgccg atcaacagga gtggaatgcg tgtgatccct ctgttgctcg ccgcgtctct
     3361 ggccaaactc gcggcacaat cgcaggaccc gtacgaagcc gcccgcgtgg cgatggagac
     3421 ttcgattgaa agccagcgtg ccgcgatacg gaaacaggtc cggtccgcgc aggctccagg
     3481 tcattcgttc ttcagcgttc cttgggccga ggagtcagac gcgccacgtc tggcggttcc
     3541 tgttgccgca caggagtgcg atcccgtgcc accggaacag atcggtccac tcatcgacga
     3601 ggtctccaaa cgggaaggcc ttaccccgga cctcctgcgg gccgttatcg agaaggaatc
     3661 ggacttccgg ccgtgcgccg tttccgccaa gggggcccga gggctgatgc agttaatgcc
     3721 cgccactgcg ggacagctcg gcgtcaccga tcccttcgac ccgaggcaaa acgtcggcgc
     3781 cggcgccagg ttcctgcgcc agcttctgga ccgctatagc ggcgacctgt cgttggcctt
     3841 gggcgcctac aatgcgggtc cgggccgggt agatttcttc ggcggcgtgc ccgcgatcgc
     3901 cgaaacgctg aagtacgtct tcgacattca ggaaaagctc aagccggctg agctcctcgc
     3961 gccttccgcc gattccatgc tcgcgccgcc gcaaccatga ccgatagcag cgcggcgctg
     4021 gccagcgcgc accagcccgg ccccatcgac tttccagccg catcggcgag gcttctgcct
     4081 ccgaacaccg gggcgaacag gaccgtcagg tgagcgacat acacaaatag cgactcccgg
     4141 ctgaccagaa agagccacga cttcccagac tgccgccgcc ggtccaccag ccaacagacg
     4201 catagaagga ggaacaccag cccaaggcgc agaaagaatg tccgaggatc ggcctgccat
     4261 ggtatttcgg ttctgcctgg cgccaacggc aactccggca gcgcgagacc gagcgcgacg
     4321 aacacggcgg ccaacccgga caggcggagc atgaacgtcc gttccatctc ccgctctcgt
     4381 gccgcgagaa atgacgatgc gcacagcgcc cccgccagca tgaacccgct ccacggaaac
     4441 accgggaaca gcgagttgag tccgtcattg aagtatgccg ccagcggggc cggcaagtac
     4501 tcgtcgaatc ggatgctcca catccaggga gccgcaagcg tcgccattat cgcaccagac
     4561 agaagccacc atcgtcggat cgtgggggat cgcagaagca tcaggcctgc cagcaggaac
     4621 agccaggtga cgcagatgca gtgcagaacg tccacttgaa tcagaggcag ccagtccttg
     4681 cgtgtgaggc tgctccaggg tttgccgaaa acggcaggag gaacgtgaag cacataggcg
     4741 atcagccaga ccacaccgat ccggcgaagc tgtatccaaa aagtccgtcc gaaagttcga
     4801 aactcgggca ctttgcgttc cgccgacacc aggaacacaa agccggaaat aaacaggaac
     4861 gacggagcga ccagcccgtt gacaaaattc agaacacgga accagccggt ttggcggagg
     4921 ccttccgaca gcatcgcatt gaagacatga gtttcaatca tcaccaccag cgcgaggccg
     4981 cgcagccggt cggcgaacag cagccggctc ctcgtcgggg aaggcacgga agtggcgcgg
     5041 gaatggggta ggatcagcgt cttctgctta gcgcggatac ttcgggtcgg ccttgacctc
     5101 gttcatttgc gcgacgtgcc gctcggcatg tcccgcgatc aaaatgaagt actggtaggc
     5161 gtccatcttt ccctggacgt gactccggag gttgtcctgc gtcgtctgga tgtagttgat
     5221 gtttctatcc cgctcctgtt tgaaggcctc ggcggcggct gcgggcgtcg gaaacttgcc
     5281 cttgggactc atctcctcag aggcctttgc cttcttgctg cggtcgcgca tcctggccag
     5341 gagttcctcg tcttgctcat gtttcaccgg cgcgcggtcg gttaccggct ccgttttcat
     5401 gaccttgtcg gtcacgaggt tgtacaggac gccctcggtc agcgtgatat gctccgccac
     5461 ttcggcgatc gaccagcggt ccggcgcggg cttgaacttc cattgcgcct cggacagacc
     5521 cgcgatcgcg tcgagaaaca gcttgcggct cgcgtgcagg ctgctcatcg cgaagtcgcg
     5581 ctcctcctct gtcaggcttc ccgctgcgag cggtgcgacg gccagtacca gcgcc
//
LOCUS       DATIFQ010000504        57282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_366745, whole genome shotgun sequence.
ACCESSION   DATIFQ010000504 DATIFQ010000000
VERSION     DATIFQ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 57282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57282
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366745"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..558
                     /locus_tag="VLE22_25375"
     CDS             301..558
                     /locus_tag="VLE22_25375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17805.1"
                     /translation="MKPIYDFLSDIGLKTTVAVWVRDPERSNGMPDKPRQSKHPSDSC
                     QRPEYQAYVKDLQARGFEIALHTVSAGDDPRSVTEAGYEDF"
     gene            complement(1190..1531)
                     /locus_tag="VLE22_25380"
     CDS             complement(1190..1531)
                     /locus_tag="VLE22_25380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17806.1"
                     /translation="MDDKVRLRNQIEVGGHSRKVRLPPVRGMQVDRTVDKSLVNDQSF
                     IRPLVGPEKFVLSQHAKIPAKSVPPELDRSVPVRLALHQVPRQPHVTKQKAKLICTAK
                     LAEKLPQQGTA"
     gene            1981..2925
                     /locus_tag="VLE22_25385"
     CDS             1981..2925
                     /locus_tag="VLE22_25385"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSB17807.1"
                     /translation="MGRRNRSVLVSTRSGEMVLRQYRSWWTQDAVLWEHSILTRLAEL
                     GFCAPRLRRTKTTDSYVALGSSLFSLVDYVEGTSYAQAIMPRSLHLELLRRAGRALAR
                     FHRTLMHFAPQGQHHLGFGPDGQDRRPGVDWYQQRLQELTSKLGTANGNSDTTYIHKL
                     VQHSTSLIDRLYHLESVLKGRVLPTVIVHGDYGLHNIVFRRGKELALLDFELARLEWR
                     LIEIVQVVSRYWDCERPDMSDQIEAIVASYVSEYPLSPDEWRLFPEVWAYYHLRATVQ
                     YWNSYWQVSPDTRRLRRALYSLRCSESGASACAKVRMA"
     gene            2951..4240
                     /locus_tag="VLE22_25390"
     CDS             2951..4240
                     /locus_tag="VLE22_25390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB17808.1"
                     /translation="MQVNETVKVTVALPVSEDVDRRAVPAGRTGSARSGNPPPRTLLV
                     IDNLEIGGAQEAVRTLASGFTRKGLEISVCALRDGHLRESIERLGVRVDILRGRTHSV
                     LALPWFVAEMIRIRRDLMRIVDERRIDVIQTHLLRALDFLVLSLRLRRRLRIFWTIHN
                     TRFSLRPDHLHRARWLLRPKQWMYRWLYRFGSRWVDGIVAVSDEVQASFQREVGNCAG
                     KTTVICNCVEVERYRCALNRETVRARLGIAGNAFVIGVVATFKTQKGHAYLLEAAASL
                     VRRFPQLHFLFVGDGELREDLLRRTRALDVEAHVDFLGIRNDVPELLGACDGFVLPSL
                     WEGLPVALLEAMAAGLPIIATAVDGTKQAMVHAETGWLVAPRSVSELEQALIELLTDQ
                     PRARAMGTAAQRRVSELFGTEPYVAKHLALYCRQQYA"
     gene            4856..5929
                     /locus_tag="VLE22_25395"
     CDS             4856..5929
                     /locus_tag="VLE22_25395"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HSB17809.1"
                     /translation="MTARTRVLCPGAPGETVRPFERGPIFLAGIDRSGIGILCELLES
                     HPSIAMTRRTNFWSFFLNRFGDLSRRDNLDRCLTAMMRYTRTRVFRPDSEQLYRSLLD
                     EKPTYPRLFALLEEGRARELGKVRWGDKSMNSEEYADTILTAYPGAKMIHIIRDPRDR
                     YASLLTHRRVGRGGVAASTALWLRSARLAKRNSLKYPDRYRIVRFETVLSQPEATLRR
                     ICHFLGEEYSPAMLTVDGPPVSAMPQPDPDGGPTPRVVTTASIGRFRNSLSPREIAFI
                     QRCTHRDMAEFGYRPEAVPLSLSGRILFYLADYPVNMTLMLCSKLHAATLKWLGRTPS
                     ARRIISECASSGAAVHYERTKDS"
     gene            5926..6903
                     /locus_tag="VLE22_25400"
     CDS             5926..6903
                     /locus_tag="VLE22_25400"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HSB17810.1"
                     /translation="MNKGPVFIAGLERSGTTLLFSLLASHPNIAMTRRTNFWKFFFNR
                     FGDLARPENLQRCITAMMRYERLAVLNTDWERLRKDFQQGEPTYPRLFALLEGQHAGL
                     LGKPRWGDKSLRTERYAQIIFQSYPAARIVHIIRDPRDRYASACKQNRIGRGKAASGI
                     AAWLWSARLARQNLMKYPHAYRVVRFESLASRPEDTLMDICRFIGEEYTPAMLTMEGT
                     PAYRDKGGNSSFEKHRPGSISTAPVGRFRKVLSPRDVAFIQAFARREMTAFDYEMEPT
                     HLALRERLAHSIVDWPMNLAYVAAWHALDAYKTRTTLQPSARRLVQSVS"
     gene            7128..8465
                     /locus_tag="VLE22_25405"
     CDS             7128..8465
                     /locus_tag="VLE22_25405"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB17811.1"
                     /translation="MPHLAQANGCHFTVGPGVSTVDGQENYAALKAGSVVCISAGSRG
                     PLVLKNLHGESGNPITFVNFGGDVEISSSGFAGIRIANSEHIRITGAGVSGRCGAGLA
                     ASEQQCGLRITAPAGRGIAGASATRHIEIDHVEIHATAYSAITINTTYEKDGVRRANF
                     TQYNTFLHHNYAHDIGKEGFYIGSSHYTSGGDDPVLRGVDIHHNLVADTGWDGIQVGS
                     AVEDCAIHHNRIYRDSLANVELQRSGIMINRGSVCDVYSNFIKDGFAAYGIYVQGNGG
                     NRIYNNVVVNRTKNSGRGITVATGSNVDRGICVSHNTLVSIEGLGINFKNAAGAGNFI
                     ESNLLADVRGEQMYVGVASATHNSSLTSVEEAKFADPSGDDYSLAVGSPMIDAALPVS
                     RCGVVDDYAGTLRPQGAAPDIGAYEFSADSAPTQPHLRSLNNGVFMAVDRFPQ"
     gene            8696..9781
                     /locus_tag="VLE22_25410"
     CDS             8696..9781
                     /locus_tag="VLE22_25410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB17812.1"
                     /translation="MTYLLPVEISRFVAAHLHFALWRPSRYFGTLLYLLGRPHPGSHT
                     RLRTFLHFAEGVVAARVLDNVGCNHLHAHFADRAATVALVASRLLGVTYSVTAHANDI
                     YVKPILLAEKIAGAAFTATCTAFNKTYLEGLLGDEAGRKIHCIYHGLDLRKYQPAVYV
                     PAGRPILLSVGQLKEKKGFSCLLKACQILRESGADFECWIVGEGPLRGALTRQIDELG
                     LREIVRLKGSLPHEDVVECYRRASMFVLPCIVGGDGDRDGIPNVILEAMAMQLPVVST
                     RHSGIPEAIEDGVSGLLVPPGDDVALASAIGRLLANPGDTNRLGRAGRISVTERFSVD
                     RNVKSLLQLFLGRSETPSSLVGQLIGN"
     gene            9791..10816
                     /locus_tag="VLE22_25415"
     CDS             9791..10816
                     /locus_tag="VLE22_25415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB17813.1"
                     /translation="MTECDGRVVVALAQMHPLFLVWGSPYRGPRSLVLARELGISDLH
                     YVYCTSRQGLLSALFKYPYQAIRTAWLLLRKHPGLVFVQSPPSLAVIVVYLYCRLTGA
                     RYVVDAHSAAFLYAAWRRPEFLYRHLARKALATIVTNEHFQSVIRHWGARAFVLRDIP
                     TVFSTQGQYSLNGRFNIVVVSSFSQDEPLRDILTAASQLPQVHFYISGDKQSAPRELL
                     DSAPANVHFTGFLPNDRYYSLLSAAQAIMSLTTRNNTMQRGACEALSIGKPVIVSDWP
                     LLRDYFSSGAVHVQNTETGILDGVLTLKRQYDRYLAEITQLRASRQREWQEKKLALTR
                     LIQSTTL"
     gene            10962..11975
                     /locus_tag="VLE22_25420"
     CDS             10962..11975
                     /locus_tag="VLE22_25420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB17814.1"
                     /translation="MRFDVVNTLTRSSWHTFVDNNPDARIFHSPEMAEVFANTRNFTP
                     TLWAALDQGATVHALMLPVDITILPWPLRAITTRTVVYDGVLCSPGVTGQLALAALLR
                     HYNAERQPRTLFTELRNSCDTSMFQSTLEQNGFLYEEHMNFFVDLTQPTSVLWKNIRS
                     NAQRNIRKAQRQNVVIEEGHNLQSVSDAYAILSTVYRWLRVPLPDVSLFQSAFRVLHP
                     ANLMHILTAKVNGVTVGALTLLLYKGTILYWYTGSLREYSEYRVSDLLVWHALQLGQL
                     HHCHTFDFGGGGRPAEDYTVRDFKAKFGGREVAFGRNVCVHSPLTLRAGKIGYSCVRA
                     LLS"
     gene            12042..13406
                     /locus_tag="VLE22_25425"
     CDS             12042..13406
                     /locus_tag="VLE22_25425"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSB17815.1"
                     /translation="MRALSVARQGKSTFGTLKRVSTILGRYGSTPTRVAESLEKCVQT
                     LEEFHCGATFPITASTLAGNERIVERYLNRGIEFAVHGYQHLDHTRLPLELQLENMYR
                     ARRLFAAGGMIPSGFRAPYLRCNGDTLTAIADAGFVYDASWALAWEVTQVAETPEYQR
                     ALSFYHSDSAVDYPSLPRWYGDVVRIPYNLPDDEALVERLSVKTEECMGRIWVDILDR
                     TWKLGELFCLGLHPERTTLCQSALLATLKQTVSKRPQVWLTTLEEIATWWLNRTRAKV
                     TCTEKGDRLVHVSVRGPKGVVILSRGVQVEAEIAEPWLGGIRLSQASELDVRASCRPF
                     IGVTPSSAPRLKNFLRQQGYIVEETDCSLTHAFVLDRPRFDETDERALLEQIDQGDFP
                     LVWLGRWPNGARSALCVTGDLDAFTLWDYASRLLPARLSTYFLNEHRLVMTPRSIRSR
                     GVTS"
     gene            13448..14431
                     /locus_tag="VLE22_25430"
     CDS             13448..14431
                     /locus_tag="VLE22_25430"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSB17816.1"
                     /translation="MARYLVTGVAGFIASKVASMLVGQGHEVVGFDDLNDAYDVRVKR
                     WRLDQLLTLDGFHFEELDIRDRRGLEETWAKLPSFDAVVNLAARAGVRQSVSNPWVYF
                     EVNATGTLNLLELCRVHGVTNFILASTSSLYGGLNPVPYSEETETDHPLSPYAASKKA
                     AETMCFTYHYLYGLDVTVFRYFTVYGPAGRPDMSMFRFIQWIAEQRSVVVYGDGTQSR
                     DFTYVDDIARGTIAALGRKGYSVVNLGSDHPLVLMDVLRMIEQLLGISARIEHRPAHR
                     ADVQATWADIRRARATLGWSPAVSPQKGIENTVEWYLENRHWAKDIQTTDL"
     gene            14516..15514
                     /locus_tag="VLE22_25435"
     CDS             14516..15514
                     /locus_tag="VLE22_25435"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB17817.1"
                     /translation="MYDEFFGLSCMPFGTSADLKFLYLTPQHREAATGLICTILNREG
                     CALLTGEPGTGKTMVLKATLTSFPVESVIICDLLIPTLSQTELLEYVMLRLGIDAAGP
                     ANKAQQLLSLERFLLDARYQGRTVVMVVDEAQSLPVDVLEEIRLLMNLEDHEGKLLQI
                     VLAGQPELDALLRQKASRQFKQRVAYRFSLRPLSMLDVESYIDHRWQRAGGTARPPFE
                     REAIRSIAAYSRGIPRLINIICNNALLAAFACGSTLIRASHVVGAAQDLDLIERGEVA
                     APRAAGTEDEIADLQRLTSGSSPSRRSTARSAAVSAGRAPASPWLVRSAKKLLLLR"
     gene            15560..16795
                     /locus_tag="VLE22_25440"
     CDS             15560..16795
                     /locus_tag="VLE22_25440"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSB17818.1"
                     /translation="MKSNTSPCHCMVVHGRYPITETRVEREARALVSRGYSVDVICLR
                     YEGESATAIEDNVRVYRLPVKRHRDSGVWVQFWEYLAFFALAFLKLSLLDRTRRYKTV
                     QVHNLPDFLVFAALVPKLRGAKVVLDLHDLMPEFYAARFRRSLCSLPVRLIRLQEHWS
                     CRFADHVITVTELWRRTLLDRGVPSRKVSVVMNVADSNIFNRNAHRALPRTSGEFRMI
                     YHGNLTHRYGVDLAVRAVARIRKQVPNIHLLIHGGGPLLAALRRQAADLGLDGVITFS
                     TTQVPTPELPSLIDTADIGIVPYRPDVFTDGILPTKLMEYVAMGLPVIAARTACIASY
                     FDEDMVTFFPPGDDEALADCIRKLYDDPVRRALMVEQSEAFLRLHNWDTESAAYVALV
                     DRLQTSSRGPIEVAEVGSA"
     gene            17510..18133
                     /locus_tag="VLE22_25445"
     CDS             17510..18133
                     /locus_tag="VLE22_25445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17819.1"
                     /translation="MQNEECGPELDPLLPELISIRESIRALEERKLAIEEQILTSLFG
                     SPSGVVEEPPETSEETLAESPSLAEKLAPRLVTPADRERAAIRISGILMEALVQYFAA
                     IEEQTLSESRKTTASLRQELEELRYNLESPTGSLATQLKQLQEELSSLQRLINGAVER
                     LNRQAEAIRSVTEVQGRHASALRQLVETLIRSAITSDIPPSQANTQL"
     gene            complement(18230..19156)
                     /locus_tag="VLE22_25450"
     CDS             complement(18230..19156)
                     /locus_tag="VLE22_25450"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSB17820.1"
                     /translation="MVTLFPVSGVEAPVWVPLVAGFVASFFTSMAGVSGAVLLLPFQM
                     SVLGFVSPAVSPTNLVFNIVAIPSGIYRYWRERRLVAPLAWIVVAGGAPGVVVGEWMR
                     ASWLPDPGRFKIFVACVLLSIGIRLIFDARRVRRDAGWEGELALSDGADWAVRKVAFD
                     RCHLVYEFQGRQYRCGTLGVLLVSLVVGLIGGVYGIGGGAILAPFFVALYGLSVHTVA
                     GATLMGTFLTSVVGVTFAELIAPLYQSNGVAVAPDWMLGGLFGSGGFAGMYLGARVQR
                     FVPAFWLKVLLAVVLFAVSSIYLAAPLLKVGP"
     gene            19312..20580
                     /gene="sat"
                     /locus_tag="VLE22_25455"
     CDS             19312..20580
                     /gene="sat"
                     /locus_tag="VLE22_25455"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HSB17821.1"
                     /translation="MSKLVAPHGSPALKALLVEGKERQEALEKAKRLKKVPMTSRESS
                     DLIMMGIGAFTPLEGFMGRDDWKGCCDDYKLPSKSGLFWPIPITLSASQDDAKAVAIG
                     EDVALWDTETSTIMGTMTVAEKYTIDKEHECKQVFRTTDHAHPGVKKVMEQGPVNLAG
                     PVTALSESYYPDMFAGIYQRPAEARMLFEERGWNTVAALQLRNPMHGSHAYLAWVAIE
                     VCDGVYIHQLVGKLKPGDIPAEVRVKAIDALVSKYFRKDRVVQGGYPMEMRYAGPREA
                     LLHAVFRQNYGCSHLIVGRDHAGVGDYYGPFDAQKVFNEIPPDALALRPLCMDWTFYC
                     YECGSMASMKTCPHESKAVIDENGQYRGGARLLLSGTMLRKLMSEGKPVPSEFSKPEV
                     IEILKEYYDSLEEKVEVKLHGFATGDSGRK"
     gene            complement(20624..23998)
                     /locus_tag="VLE22_25460"
     CDS             complement(20624..23998)
                     /locus_tag="VLE22_25460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17822.1"
                     /translation="MATFPITTLTPAAVQQNLQNNGLGALGLTTVRLSPRWANTTPAG
                     GDYDSAALTLMITGDIRAPFLGIREHAFTPVSSTLANAASENDNTITLAAGTGANFPS
                     PTGGSVFLVLSNASGSKREVVGCTARTGDSLTVSRGLDGSAKQSFSPGDKVTLRLMAG
                     TRTTEFYGADGIPLTGACTVLRLHPQALLRLERLAFARYAQPGDPPIRPVPWAMVVRG
                     VEGFSTARWFEPDEVMTGISGAISFHDGRGQIIDPIYVACLFDDLLNALPGLQPTTVS
                     GPATGAGGVNQIGAIGGAGTLLHFVDPHGGLYRPALAGATMVTADAGSNVTGTVPASG
                     LITLAAGDRIQGANTDGGRLRWGWGTFGTMGRAPLIPPALPGGPAPAPALPKQFFRVV
                     VADVDWMLLGNRTSAAVLGVPADDQRIPADLLPQVRDRVTIDYLADGPDTLAEANAVL
                     NLPSQAMVLAVSSFIDATLQTPPQRGPNGHWPAFPAPNTNLGFAAPPALPAGSVTAAF
                     TAANDVVATIAAGIAPNGAHIRIYPQQFVEIAAIAEEPSFLRGDGGASISTGGAVQVF
                     LRNPFRLASGQPPPSPAVLTMDIVIAPRVGRRQLFGAVTANVAAGPVAAPADPFAATP
                     GTTILSVMLPQAEGVAESPLFGIPKTAPPPAGPPPSNFLELVLSLAAEPSPRQAPRLP
                     TMARFDTVVVAGATGGTPAGSLLWDGVLSGGRLSGESRSALHESGNPGNPAGPDVHAS
                     GVRVTGALGYDLARLALRRAQPIIPLPGTVGANPGWVVASLGDNFNVPDDSASVATGI
                     GVLLDTVAVGCETPWLSSLTPPPPGNTVQQMINDGAAAMGLPPPPITITIQNEPRVQR
                     EVRREFFVSKHGLRDAQYSLRRAIAEARELVYIESPQFARTARPAGPPQPFEVDLVAE
                     LATRLSQAPNLKVLICTPKEPDFATNFKGWWRQHYQARSEALGNLLAAAPDRVALFHP
                     VGFPGRTAFIRTTSVIVDDVWCLVGSTHFRRRGMTFDGSSAIASFDRQFEHGYSRNVR
                     EYRRVLMAAKLRVAAPVAGAAPTGDWLRLGRPASAFDLVADLLAQGGLGRIQSLYAGP
                     ADTTVLPATADIADPDGSNGVTFFNIFSGMLNEAGD"
     gene            complement(24003..27638)
                     /locus_tag="VLE22_25465"
     CDS             complement(24003..27638)
                     /locus_tag="VLE22_25465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17823.1"
                     /translation="MAFIVDTTRFQAEGAFLNWAVVARRLGLLDVARTEAARLGAVLE
                     LRWMIHRDMGFPSGPFTVWRRLRQARAPVPLAIDVGSLDFFFGTRLVDWHDTVSTVEI
                     DVNGAAGLVMAFAGAPLLSQVVAFTSAPGGPATLQLSASAMDGLIVSAGINVLQVRGI
                     PADDLSAAAGWEKFELVGLPVSKPEWSGVGTHADNQGMVAALTSPVNAARQRLERGAP
                     PVGWGPLLDTGVPAPLWSAPDFTDLIKDLNTDLLQFLRPVVANFPPNAHYEQQLNVPL
                     PPPENSSGQSMPVAGGTSKVSPLGLTLIGAGTDPYFSLGLGFGTAYPVTTQSFAAVNI
                     PNAAQYDYMITARWDKGFDGNSAPVEYAALVPTPQQAIPPPAPANMMTRFMGHLRPRQ
                     TDFDWLCSVRVGWDRPPAIPLFRPRTYAFARAGVAPAEPTDAVMAKRRSGGFLPIAIN
                     ETITPPDPENWRIHAVHRELPIPSNPGNRTMKYAAAHQDIYGQWSTWSAINRTVSQPA
                     VDQVRIVSAQMHASVPASGTVCPGTLVIEFLWDWRIRRPQTIRFAGRLYAAAFHGDPP
                     PIVTVPSGLQRSLGGADPVAEITFAGDVPSSTAGTLQGLNQAGDTAVSFGPPQGSETR
                     RYRITIPGFSLNYAATGHIGLALWAQGQERIAPQRTGAWSAEPSVISVSDPRPPFVPP
                     DIVTLASLPDASGECHARLAWSATPGATGYFVYESTETKILLANNLSEPAQDLTLSQR
                     LTRIKNGFKANPSRREFTRRNAKLVTGTSMDVTLPRGSTAIHVFLVLGVSAGQVEANW
                     PSGPNADDMLQAFAAPRVLAPAAPTIEAQSFLDQSVIPNVYRARIRIGTRKGPRVRRL
                     DLHRVRVDDAAKQLETMGPPVQTLNVASAGWTVQQETDTLGSHITVAQGFDTPLGSWK
                     RVWYRATAWSEKDDLRGNLAGKSPASSVSWVVVPPVDPPVISALQVEWPSGGGPGDVL
                     IKWASPVPVKKTPLGPHTISIRAAVVGAPAGTLPLISVDGPLESLGVTQPGTGSGAWR
                     AGGAPPNPIQYRAIIRRADVNDAVQFSARITDPLGRSSEQRTTIKPGSVLPLPVLEGF
                     VLTASVAPPGTMLSWFTDAPLDAGDAGSYILRVTAIRPPKQIFPPIGPFIPQPPITIE
                     LGLGDIALDEPGPVPPGVDPLRVRRNSGAGPKLSYYAFCRVPVKQFVVRLTSPDGHFA
                     EHVQPVI"
     gene            complement(27640..34767)
                     /locus_tag="VLE22_25470"
     CDS             complement(27640..34767)
                     /locus_tag="VLE22_25470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17824.1"
                     /translation="MPFDLSLLDLITPYILRGDSFGNWHAALSVLLVREHEIAVDEAG
                     IVIRGVVSFEGAVRPFIDPTRMIMGIQAENTEGHPQNDPARRDPWIDVRDTKLEFQLV
                     VPRTASQKVSTAVTAIGGDPGFATTAAVLTAYDTNPLDAPPSDYATTEFVLDFLLTTI
                     VLRPPFLRGATRQPDGQLVPDPAHEQVKITLPRIKMRLTQGSGNNDPIAATLLSAGAT
                     GLDDPGDIAVAELIKMDPPYAFIGPSNVVGFGFRSGVLDLSNGSTPPDVLSQFGFDES
                     WTGLYLPEIRLFVAPHGAKDFAVDAGVRNLLIGIGGSSGVTGDFELTVLNQGAGPLKL
                     GARFYDADGRGYGITRASDTTATAQLPVRTRMVIDVEGGRTPFTVTAAFDGGVAQAGR
                     EFDVDLGTQTTRLIEIHATDTSAPAKSADLAIQVSRRPSIAAPPAGSQTQPAAPAVEL
                     QTTSITQGGTPVLAPRLRLVTETPATATIALDVDPSRAAFTQWTVNGNPAGTSATLTV
                     DVAPGTQVTVRAELPGVAGVANFTGYYRFDRPDPGTDTATRSYALDPENTHTEPSPDE
                     GTTSPWMGGTDVASALRPVLQSLPDGTAITIKGFASYEGDSSQQKVTYNTDLARRRAL
                     GLRAIVEQLQTEPSLSSKHFTLSHAPDMTSWTNQGFPDVSTRRIWWKAVASWPPSSTA
                     GTITEGLVKRAPVEQPSGPPPTIKVEDPPPATPPSPPSWFRMLGCKVRIVRNTFVACE
                     VFGKFDIQTAAENRLAGNIGANPQLAAGRPLGANNTGDGLIDVRVVVQIDDATDTVSV
                     IGYYGADPADIDGLYMWGTLPNEPVAADPGFALNFFGTSIVFMPLLSAGAGAVANDGA
                     LAELAVTGAILTVPAAIAALGWVKVERVIWYGGEIAVSVRPTGPEVSVLFDLESAISM
                     NVSIGGKTLIEISREAPLAVRYKAVGIRVGYEPGQPAFQFRPIFDASKGYTIDVSKPG
                     AIRVVEPLGQILQILGARIARNNPLIFEVDLGFAIDLGVVSIERARVRLRFDPVLPPE
                     LTAFAASVDIPGALRGRGYMELNENEIKGQIDLTIVPVQVRIAAGVGVANIPPAQGGP
                     ATGVIITLEVEFPVAIPLGNSGLGIYGFLGLFAMNYARNEDAIPASDMAPALAWLKAT
                     GGEPTKIDFWKPRVNTWAFGVGAILGTMGSSIIFNLKGVILLELPGPRLLLMMKANLL
                     KVMPELGGTAEGLLLAVIDLDMGRGTLTIGISVEYKVSPLLEIRIPVEAFFNFNDTKD
                     WHIFLGRYSDQIHAKILEVFEGSGYLMLSGNGISGIPDLPAVTGFSIATGLHVSFVWG
                     SKSAGLYAELAAGFDAIVGFDPFRLAGILYVRGTLHLFIIDISAWAKLKVDVGEDAAH
                     NKISRIEGEICGRIEFLFFSIEGCVDFALGDPSVPVPDPPPLVKSLKLISRSPALVVG
                     TGTDKSIDGGIGDGVEAGAAPAGGLPVVPIDAIPLLMMSTPPLAAAGVQFEGQNLNGT
                     PEAPPDGWVPRGDVFFKYTLTEVDLIGSVTAGATPATWWAQKSGDKPLEAQLALLSWV
                     PEATPKAIESSKFLEETVKETWGTVCWPAAPQAPVFWDFLREVLGPSPIGWILEGEAW
                     PNPPNTVRSQDPDILLKVTERWRCGDSKLDVLTGIIPAQVEGAAAHCPSRRPDTRPST
                     TAPVVAPVTTTAVPVLAEALTPAIATTALTSAAARPVLDVVGTARGIKSTEVVSEERI
                     NLVEAVRRINNGIPISRSSLALLVAGGIEPQAGAAGTAGATVPTGEKRCFTRALAAPV
                     FDSRKPQPFGDPQRAKQIRDGWKKLKFKPGPFDDAVVFHTGRVEYATFYLLVPGRVLE
                     GQMLMIAECDANENVLSQRLVTPADRVPPNMFPARWTDPAGPWRDDMQLLVQFQTLSQ
                     VQGYSAVVVMVKGNPDTATIHVGLRPQDPKWHRAFTKRPFYVVGLEALRYGETLRFDY
                     DTLEQETKKGVLETALGPESADQALLLPNTVYGVHVKWDASRERRPAGQPATDHKDVS
                     NQVQKFWFKTDAEAPRRLDPWMLCSTPEDSEKQYFGAEVIKIVFGTNNVARIYDAYNR
                     RLQVRIKAASFRPTPSSPAVPHPFPINDVTLQPVKADVLSPWEDAVVGLVTDTCVPVD
                     GSRTRHSMVTVPIPLEPFTDYVLDIECVDKAAPEGTVGEFVWRRSFSTGGFATTAAFA
                     ESFGIAAVQHRFAATGALQAIGVKFATSAPKGSELDQEFTAAGFEPLPVPKFPRVVVF
                     WEEGVPKPQPAAVLIDASEPMWRTRSIPREITDPGPAANKRYELTPVEWLALSEQAGG
                     DPVVDRIVRAPGGQRALITLKPNSRGKHIRLALKRIAQKEPYLDGPAATDQFLSVADF
                     IFVKAPWEEED"
     gene            35159..36034
                     /locus_tag="VLE22_25475"
     CDS             35159..36034
                     /locus_tag="VLE22_25475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB17825.1"
                     /translation="MLNRRTFLGGLPLLAARPEPKATDVWSPPRCEVWDVHCHIFEVA
                     GETPEARVTELVKFGDRMGIERFVLFMGFPFYTDPTPEQLRQQNDQVLRAMRAFPQRV
                     SSFVYLNPNYLSFSLQEFDRCVRDGPMVGVKLWVAKHCNAPELDPIVERAASLKAAIL
                     QHTWLKSTGNLPGESTPFDVAELALRHPNVPLICSHVGGDWERGIRSIRGTKNVYIDV
                     AGFDPTAGAVEMAVRELGPERILFGSDVGIRTFASQLGKVMGAEVSDSARKLILCGNL
                     KRMLTPILKAKGRRL"
     gene            36031..36804
                     /locus_tag="VLE22_25480"
     CDS             36031..36804
                     /locus_tag="VLE22_25480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB17826.1"
                     /translation="MIIDTNVSLSRWPFRRVPGDEPADLVMRLRGRGVGLACAGSFDG
                     ILHKDLAGVNTRLARECRTYGKELLLPFGSVNPKLPDWREDLRRCREEHRMPGIRLYP
                     NYHGYDLKDPAFGELLTLASAGGLIVQLAMRMEDERTQHPLLRVPPVDAGPLPDLIRG
                     QPGVRIVLLHWKGLVGPALLERLATAGEVYFEISMLEGLGALARLIQQVSAERVLFGS
                     NFPLFYFESALFKVREAGLTQLQQNAIFEGNAQRLLHLR"
     gene            complement(36869..38506)
                     /locus_tag="VLE22_25485"
     CDS             complement(36869..38506)
                     /locus_tag="VLE22_25485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17827.1"
                     /translation="MNVHNHGFCGIHGPVLEGVFRAAGFDARSFSVPAWNHVATEIFY
                     GGGWHYFDVDLRGTLLRPGGVVASAAEARADPSLWSDPRHTVEPFFPNVKDKGVGFRM
                     FSAKPIDLQYHWSVGGWIMDYVLRPGESLTRWWQNRDGRWHHSPTYTGWMRQLLEKPP
                     CGIKPNNANFSIWGVGSSLLLYRPDLTEKTRDVELGAYEVRNLRTSAAGLTPAERGSG
                     FAVFEVLSPYVIVPVMDDLDDPAGALNRDAATIMIDATTAVEGDISTDGGHTWVSLGA
                     LPNKLRYDLTRFVCGRYHYFVRLGMQGEPGAAVLRSLELRTWGQVAPISLPRLAKGRN
                     VLRLGVNDPRGRPTRLLPMLPHLGDPEDIRRHGIWIVGDYQPRQPSQRLRGQAVAPVE
                     AGPGQVIEWLSVGGMFRAHHLKRAPLTANKIEIATALEGPFRTIYGGTGTVPDWNQHW
                     HYSMDTDVILPEPVRKVWVRYTGDPSLNAIRIYAHCRDPRPVNAGVVRVTHDYSIGDE
                     RILKSVEVPAGGGSYTVDCPEAPTNHALKLETPHSMR"
     gene            complement(38836..40848)
                     /locus_tag="VLE22_25490"
     CDS             complement(38836..40848)
                     /locus_tag="VLE22_25490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17828.1"
                     /translation="MRSVLVIPFMLCSLYAQQSAVVEALKKPILGLRQGTLEVQVYTG
                     AKVLPVPHFRTAAEWESYAAKLREEVLAKAILRGEAARWNEAKTNVEWLDAIDAEGYR
                     VKKLRYEALPGLWIPALLYEPAMLSGKVPAVLNVNGHEEIGKATPYIQARCINLAKRG
                     ILALNPEWVGKGQLDIPDFDHARMNQVDLTGTSGVGIHFLAQKRALDLLLAHKNTDAN
                     RVAVTGLSGGSWQTIIISSLDTRVKLANPVAGYSSYLTRSQWPEPDLGDSEQTPTDLG
                     SVADYTHLTGMLAPRPALLTHNSRDNCCFRADYAVSPLLMAARPIYELFGKPGNLRYH
                     INYDDGHNYGQDNREAFYRALRDYFFAEDPSFSIQDIPVEKEIRTRQQLNSPLPADNL
                     SFHQIAMNLSRQLPRRPELPEDQRAAAAWKETGRSKLRGIVRAYDFPVEAIEAGSDTP
                     QGADGLKATYWKLQMGEAWTVPAVELASGTHESTALVVADGGRAGSAAEIERLLAGGQ
                     RVVAMDPFYFGESRIDKRDWLFAILLAALGDRPLGLQASQVVAAARWLQSRNAGPVTV
                     VAIGPRSSLFALVAAGIEPKAIASLQLHESFGSLKEIIEKNMTVRDAPELFCLGLLEE
                     FDIRQMVALVAPRPVKVMEPSERAKAELAGLAAFYGMLRVRFDPVN"
     gene            41116..42486
                     /locus_tag="VLE22_25495"
     CDS             41116..42486
                     /locus_tag="VLE22_25495"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HSB17829.1"
                     /translation="MKRREFLGLTTMSLSSRARGQRQERPPQPNFVFIMADDLGVYDL
                     GCYGQKLIRTPNIDRLAAEGTRFTDCYAGATVCAPSRSVLLTGQHTGHTTVRSNASLR
                     TGRRVSLTTDDVTVAEILKGPEWRDAFDDARGYATAIFGKWGLGEPGTPGLPNDHGFD
                     EWFGFLNQQHAHGHFPDYLWRNKTKETLKGNLGGGRREYASDLFAREALYFIERHMYE
                     PFFLYFTPTVPHARFEVPTLGPYANESWPEEAQNYAAMVTRMDTYVGRIMAKLRETGL
                     DENTIVFFTSDNGGIFEFKPFGTNGPLRERKGSIYEGGIRVPMIVRWPGRIKVGAVSS
                     FPWGFCDVLPTLADLAGARGLPKNIDGISVAPALMGKAQSAERPLYWESYEDGGFHQA
                     VRMGRWKGVRHGLTRPLEVYDLQTDISETKDVAIAHKNIVTRMEEYLRNCRTDSTEYP
                     IDRKAN"
     gene            42650..43585
                     /locus_tag="VLE22_25500"
     CDS             42650..43585
                     /locus_tag="VLE22_25500"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HSB17830.1"
                     /translation="MKFRFWHVMPLAVSLSAQVNITRQAGEIAVEIDGKPYTVFYLAP
                     GGNKPYVYPLRTADGLVVTRHFPMEEFPGEVKDHPHHRGMFFSHGDINGFNFWATEPN
                     SKSPKKASMALKKVLEAKGGKKSGTIRAMFDGLDPMGKPIMTETRTLVFHSGPKLRII
                     DYEIQIDPLETLKFGDTKEGTFGIRLATSLQEDKGGRMVNAEGRETEKNVWGKRSAWV
                     DYCGPVDGQTVGVAIFDHPSNPRHPTYWHSRAYGLHAANIFGVHDFENDKTKDGSMTL
                     NKGQTLRFRYRIVIHPGDTKSADIAGLYREYSTGK"
     gene            43614..44798
                     /locus_tag="VLE22_25505"
     CDS             43614..44798
                     /locus_tag="VLE22_25505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB17831.1"
                     /translation="MRRHPFGLGAIGVLLLAGVASTLGQYQQKNAKEWNKDPEWQEVR
                     YGAWGGPGVNPAPGAMDSMLLKDYAPKSSVVAQRTSVPKAKYPAIDVHTHVLARTPEE
                     VADWVKTMDEVRIETSVVLTSATGENFDKLVDLYLKKYPTRFQLYCGLLTTDMDNPDY
                     PARAAAELERCYRKGARGVGELSDKGWGFSRNTNLPRDKRLHPDDPRLEAFWNKCAEL
                     KLPINIHVSDHPSCWQPLDVYQERTPDYQHFNQYGKDVPSYEELLAIRNRTLARHPKT
                     IFIACHLGNQGNDLAALGKVLDSYPNLYLDISARDYEVGRTPRAAARFLGKYRNRILF
                     GTDMGREKRMFRAWWQLFETADEYIPGRVSWRYYGLELPAPVLESLYRGTARKVMNWE
                     KL"
     gene            44833..46152
                     /locus_tag="VLE22_25510"
     CDS             44833..46152
                     /locus_tag="VLE22_25510"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17832.1"
                     /translation="MDRRHFLMSSVAASAAMRPSMLASPNDTVRVACVGFHGQGQAHI
                     SGYLKLPNVELVALCDVDEAVLKDGMKMVEKTGKKRPAGYTDFRKLLEDKSIDAVSLA
                     TPNHQHTLQTIWACQAGKDVYVEKPCSHNMFEARQIVAASRKYNRIVQHGTGASASLH
                     EAAQQVREGIIGDVYMARGLCFKVRDTIGRTPEEPVPPGVHYDLWLGPAPKRPFTKNR
                     FHYNFHWFWDYGNGDFGNQGSHQMHAARFVLGVKYPTKISAIGGHFLFDDDQETPNTM
                     VASYEFNEGGKKKMLVFEVRHWLTNNEAGIGDRSDAFAGSAVGAAQTVGNLYYGSKGY
                     MVYAGGFRTFLGKEQAPGPKSSLGRRESAFGNFVDAIRSRKREDQRAEIEEGAISTML
                     VHLGNISYLLGRTLHFDSNTLTCIGDAEANAMFTRKYRPPFVVPKLV"
     gene            46198..47769
                     /locus_tag="VLE22_25515"
     CDS             46198..47769
                     /locus_tag="VLE22_25515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17833.1"
                     /translation="MFRHRMTVTAVLMALGCCGVEATLRAQRPVPVEIRIQADRTVRR
                     MQGGIGASFHAIEEPKPGQRPTGGVWAGSAWGANPPAEDEAAWKSLFHYANWLGLDWC
                     RVELEQRMYEPRRRRFEWDNAEMRILYRILDWAEKQGVDVFLQQMWSNVEWNAYPEMR
                     ATMEGRLRSAPLSIDDFAYGLGELLQHLTKVKGYRCIRWLSINNEPGWDWSWWQGPDG
                     QPVPITPAFAAVRKELDRRGITVPLSGPDWTDLPDLEPERIDFDSYIGAYDLHSYNAY
                     FDGIRFGYPLSVAEKRLARWAQWAHGRGKPMFLSELGTMAFGFVGKEPGPGVYESGLK
                     DAALVVRGIRAGVDGFNRWSFINRGDLDGQWQMVDTWDIDEGKLKDHFTPHPNTYYLF
                     GLLSRFTAKRSDVLPANVEGKFDPGARQLAAAVLRSPRGHISVLVVNEMYWDADAKIT
                     ITGLSGRTLLYRYSITGADRDRTDVVVKPAAKFDLDASRSSVTDRIPRLSVTVYSTYE
                     LDHGQPGVMAENDSK"
     gene            complement(47804..50371)
                     /locus_tag="VLE22_25520"
     CDS             complement(47804..50371)
                     /locus_tag="VLE22_25520"
                     /inference="COORDINATES: protein motif:HMM:NF027656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HSB17834.1"
                     /translation="MRRHEEYFVSTLVLLVLTTIGCGGKKYAWTRSAAVIEREPGVEE
                     LAAYPPDGQPLRVNPPGFTWTPNEKAKTYRLEVRRSSESSSGILATEPQSSTVYAMSH
                     AFEPGDYVWQVVYLDGAAVTGASKARRFTVPKGLAPLVMPDVARLKEQLAKVRPRLFL
                     SGKRLAEVKTAAASGNLPRWKAFLEEADASLAEQPYAEPAGYPSAEFNVDEWRRIYTP
                     AKAGSSHLARVALAYRITGDKKYLEGARRWMLALASWDPRGVTSHKVLQPNGSEGNDE
                     ASMPILDRMSLAWDWAGSELTPSEKAKVLAAMTERGNQVLELLKKIDFLTHPFSNHEG
                     RVLAFLGNAGVSFLGDIPDAEKWLDYVLRCYLTAYPGWGGDEGGWAQGLSYWGGYVNY
                     HTGFAEALRGVTDVDLFRRPFYRNTGYLAVYCIPPYAERGAFGDGSDRGPGGSQRGIL
                     ERLAEAFDDPVLRWTAQSIPSRQQRGGSQRSTPAQFGIENVGSVLRIGPAGGGEPKVE
                     PPSKLDGSRFFTDIGWVAMHSALGDAREDVWSLFKSSRFGSFSHSHADQNSFQLNAYG
                     RALLIDSGYYPWYGSPHDQLWTRQTRAHNAVLVNGRGQPPFEWAANGTIEDYIREGVV
                     TMARGQAAKAYNIPQRESVKKQWSEWLKEPIPPMEPKVESFERTVAFIASKTRPVFVV
                     HDHLRTDAPATFDWLLHALNQMKLDDKAGVIRVQNEDARLVVRLIATEPFRFSQRDWF
                     PIQPELDEATARTASLAARRERFANQWHLNARTGKPVREIKFVAVMVPYRASEAEPEI
                     ALEKAGDTAVFKVADSAVAAWIGAGAQGTLAGGGIDTKGRLAVRVSEGGKMETVVTP"
     gene            complement(50443..51123)
                     /locus_tag="VLE22_25525"
     CDS             complement(50443..51123)
                     /locus_tag="VLE22_25525"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB17835.1"
                     /translation="MSRLSVWSALLVLGCMSMAAQQKQLPVAGFDARWEPLFNGQDLA
                     GWKVVGPERWSVDDGTILGEGIHGKDGFLKTLKQYRNFHAFLRFKCETPNNSGFFYHS
                     DIEEDISKIKFIQVEISSSVGGHTGGLHGDHTPETKGRGWIVWPAPELETVLRPFDWN
                     DMLVQVEGHRVRTYLNGVRMIDFTYPDPRNTNGVVALQIHPGKPSRIRFKDIWIRDLD
                     ERPGQTGQ"
     gene            complement(51212..52321)
                     /locus_tag="VLE22_25530"
     CDS             complement(51212..52321)
                     /locus_tag="VLE22_25530"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17836.1"
                     /translation="MRIDRRSFLLGSAAAVRVVHGQSGPPVTTAVIGVGNRGSYLLRG
                     VLAQENAKVVGLCDTKPDRLDKAATTAARDNPATYADWRRIIDRKDVDAVFIATPPYL
                     HSEMAVAALKAGKHVYCEKPIGVTAEQVRELVKAAKSTNRVFVAGQQMRSERQLAETV
                     RKVREGAIGDVIMVKAQRHASGDLNHTGSSADWYFDVTKSGGYLIEQSVHNLDACNWV
                     IGARPSRACGFGGTQMFKNDPPGRTIFDCGLLVFDYPNGVKMSFTQNVFHPRTMPNNN
                     QSVYVYGTKGAVDLLYTAMMYPLAREGQPSALTEKQQDPPHAHITTFYDCVTKGAASP
                     ADITVGATAALTSILGHEAMVREKVVNWADLGVDL"
     gene            52727..53461
                     /locus_tag="VLE22_25535"
     CDS             52727..53461
                     /locus_tag="VLE22_25535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17837.1"
                     /translation="MPAGTWITVRIDQELSSDHNLPGDGFTASLVQPLVANGFVVARR
                     GQTVGGQVVEAQKAGRSKGTSRLGIALTELSLVDGRQVPVTTRLTQYQGGSSVGRDVG
                     AVATTTGAGAAIGAAAEGGFGAGMGAIAGAAAGMIGVLSTRGKATVIYPETPLTFRLE
                     EPLTISTESSARAFRPVVPEDYPQEPTLHRERRVAVAPPPPYYYYGGWDWWYSPYYYP
                     RFFYGPSFYYFSGPRIHGGHRGFRRW"
     gene            53563..54069
                     /locus_tag="VLE22_25540"
     CDS             53563..54069
                     /locus_tag="VLE22_25540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSB17838.1"
                     /translation="MPLEWNVTVAAMETDDWSAVRAIYLQGITTGNATFEKSPPTWEQ
                     WDSSHLTVCRFVARSQGEVLGWAALSPVSGRCIYAGVAEVSIYVANGTQGRGIGLALL
                     STLVSASEQAGIWTLQAGIFPENTASIALCKRAGFRTVGTRERLGCMEGRWRDVVLME
                     RRSSVVGI"
     gene            54184..55683
                     /locus_tag="VLE22_25545"
     CDS             54184..55683
                     /locus_tag="VLE22_25545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17839.1"
                     /translation="MERRILKCQSMAREHRAEHAITRRHLFGTMTGAVAAAWPRAAVP
                     AETKGDPRLTAWIFMQSPLERWMPDFHRTFDAWEDGGVRGIALGYMRFQRENGTVPSF
                     PSNPDVYRAFGVSPPPAYPHDPAKARQFHAMLDDAAKRGWEILLFGLGRLGGSRPPAE
                     DPFDAAGFAATVQDAINYYPQANGIIIDGPGEHHYELAFHHGGELLEIRDSERPRLEL
                     LGFDLARLEKGIAHLRSRLHQLTPALVRYHSPGGLIADLALFDVNEDVLYWLRTRQDV
                     ALRHMAAIRKQIDGLNRKVKLGGIPRTAVFSAQTAQDYVRMSPYFDYWFPKHYFWHRG
                     FDGMYGTIARWVQTIKEWNPILTEEDCFAVVKSWFGIELPGVHSLADLEMGFPDEFFS
                     RVVYSETRRALEAVGDPDKVIAWVSTGRLPHAGDPMPARDLHNILLASRKAGLRRFLF
                     HPDPNLGAAEWSVISSMCGKRWIEDPSGYWPPDTPRPDSFNGARKRPPR"
     gene            complement(55711..>57282)
                     /locus_tag="VLE22_25550"
     CDS             complement(55711..>57282)
                     /locus_tag="VLE22_25550"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HSB17840.1"
                     /translation="SVERKFVLRAGERRELTYELKRRTPLRMEGWYTGDNHDHMVHGE
                     RMVTVDFAYAALAARSEGLDYLSLAHLWNLPQATPEDLDRECRNASTDDFLLTWNLEA
                     PKNYYRGDVSKCLGHGWTAGMRGRTEDGRDAIGELLALSAHDYESDKDPAANFESHAL
                     IHSLGGVVSYSHPCRWWWGKWGGRGIYPVEEKKFVSNMAAELPFDTVAGPTYDTIDVL
                     MQPHETAANDCAQQLWFLLLNKGYRIAASGSTDATFDRPGGGVPGRVRMYTRVRGGFS
                     LAAVAEALKTGRSLVTSGPLVMLEMNGYGSGDVVGIPTCGGGPCLVSIKGRIRAWASG
                     AATSGLRKVELIRNGVVVKTFEPVSGRTEIESGFNVVERERAWYIARCFGADAGHIAI
                     TNPVYFEPEGSRPPEPVPARITAVVKDGITGGPLRGRWEVIRMVGKEAIAETAADFVD
                     GSFRLEARATARIRVTVPGYEPLTKSIFLDSPALLESTFRLRTEDLTDWRTFERMREL
                     LQDVRLEFAVKKLTR"
BASE COUNT        11322 a        17205 c        17340 g        11415 t
ORIGIN      
        1 ctttgaccat ctcagccccg gtgcatgttc tccctttaga gacgcctccg aaagcacctc
       61 ccaatgcctg acaaacacct tcaaccggtc tcgcgctggc ccagaactat cagggtcctc
      121 agcaagcccg gtcgtcacag catcatttcc gtagctgcgc ttgcacttct agccatcctt
      181 tttatcctca cagccgcacc aactacaaca cacggccttc cacgcctttc cccgtaccca
      241 gatggaaaca actttgcctt cacaattaca gacgatccgg actactccac gtttgagagg
      301 atgaagccga tctatgactt cctcagtgac atcggcctca agaccactgt tgctgtatgg
      361 gtccgagatc cggagcgatc aaatggaatg ccagacaaac cgcgacaatc gaaacatccc
      421 tccgacagct gccagcgacc ggaatatcaa gcatatgtca aggatttgca ggcgcgcggc
      481 tttgaaattg cgcttcacac tgtgtctgcc ggagatgatc caagatcagt gaccgaagca
      541 ggctatgaag atttctgacg gatgttcggt cattatccgg ctttcaacat agtacataac
      601 gacaatctcg aaaacattta ctggggtcca tattttcgga tggctttaac gaaaagacat
      661 ttttggaact tactagtcac gacaacgtgc gacggctcgt ggaggagagg gacgcttcaa
      721 ttgtatagaa tggcaacgcc gaggccgtcg atggcgtcac gttactcgca aggcccggcg
      781 aggtgtggcg tcacgcggat ggaagccttc aaactgcaaa tgacgaaggg gagcttgtac
      841 ttggcaacct tcgtgccgga gaggttcttt tgttgcagcc tggcgctgtt gaagagctac
      901 gggtggacgt tgcgcctggg tgccgggaga agctaagaat ggtcgtcaaa aggtcgtatc
      961 tgttccttaa acatatggga cgccgggcct agttaagagt cggatagaac gcgacatggt
     1021 cgtcgagctt attggcacac cgggcgcagg taagacatcg ctcttgcctg tggtcatgga
     1081 ttctctgagc gaacagggcg tgaccccccg aaccatggtc ggggcaagtc gcgtgtacgt
     1141 cgcacgtacg acccttggtg cgtacatcgc ccgcttttcc ccgccacgct tacgccgtgc
     1201 cttgttgtgg cagcttttct gccagtttag ccgtgcatat caatttcgct ttctgcttcg
     1261 tcacgtgagg ctggcgcggc acctgatgca gggccaggcg cacagggacg cttcggtcaa
     1321 gttccggcgg cacgctcttc gctggtatct tcgcatgctg ggacaatacg aatttctcag
     1381 ggcccaccag cggccgaatg aagctttggt cattgaccag ggatttgtcc accgtgcggt
     1441 cgacttgcat gcctcggacc ggggggagcc tgaccttgcg gctgtgtccg cctacctcga
     1501 tctggttcct cagccggacc ttgtcatcca tgtgaatgct ccgctagagg tgtgcgaagc
     1561 ccgggtcttc gatcggggtc cgtgggccca tttccggggt gagagtccag ccgcactctc
     1621 gcggttcatc acgaattctc acgtcgtagt tgatcaggtc gtaaaacgga tgcgtgcgaa
     1681 aggctggaat ctgattgaag ttgacaacgg cagcgaggga ctcgcccttt cggcggcccg
     1741 tcttcgctcc gtcctgatga atcctgactc tacgtccgtt ccgctacccg gaggaccatc
     1801 agttctcccg acacggtgtt catgaacctc ctctcctcat ttccatggcc tccccgtcct
     1861 tcgcggatct accttcacgc tcattcggtc tgccgccctc tcgatgtcga caggaaggtg
     1921 attcaggatg tcctggggga gtacgatctg gaacccactg ggcatcaccg aaacctcccc
     1981 atgggccggc gaaatcgcag cgtcctcgtg agcacccgat caggagagat ggtactgcgg
     2041 caatacagat cgtggtggac gcaggacgcg gtactgtggg agcactcaat cttgacgcgt
     2101 ctcgccgagc tgggtttctg cgctcccaga ctgagacgaa cgaagaccac cgacagctat
     2161 gttgcgctcg ggtcatccct gttctcgctg gtcgattacg tggagggtac cagttatgcg
     2221 caggcgataa tgccgcgctc attgcatctg gagttgctcc gacgggcggg gcgcgccttg
     2281 gcccgcttcc atcgcacgct aatgcacttc gcaccacaag gacaacatca tcttggcttt
     2341 ggccctgatg gtcaggacag gcggcctggc gtggattggt atcaacagcg gctccaagag
     2401 ctgacatcca aactggggac tgccaatggt aactctgaca ccacttacat acacaagttg
     2461 gttcagcact cgacatctct tatcgatcga ctatatcatc tcgaatctgt tctcaaagga
     2521 agagtattac cgacagtgat tgtccatggc gactatggct tgcacaacat tgtgtttcgc
     2581 cgcggaaaag agcttgcgct attggatttc gaacttgcac gcctggagtg gcgactgatc
     2641 gagatcgtgc aggttgtctc gcgttattgg gattgcgagc gtcctgatat gtctgatcag
     2701 attgaggcaa ttgttgcgtc atatgtctcc gagtatcccc tgtcgccgga cgagtggcgg
     2761 ctattccccg aggtgtgggc ctattatcat ctgcgggcta cggtgcagta ctggaattcg
     2821 tactggcaag tgtcgccgga tacacgcagg ctgcggcggg ctctgtactc cctccgctgc
     2881 tcggagagcg gggcatcggc ttgcgcgaag gttcgtatgg catagagacc ggcagacccg
     2941 gtttgaacca atgcaggtaa atgagacagt gaaggttacc gtcgccttgc cagtttctga
     3001 ggatgtggat cgccgggccg tccctgcggg tcgaacggga agcgctcgct ccggaaaccc
     3061 gccgccgagg acgctcctgg tgattgacaa cttggagatt ggaggtgccc aagaggccgt
     3121 ccgaacgtta gcgagcggct ttacccgtaa aggactcgag atatcggttt gtgctctccg
     3181 tgacggtcat ctccgggagt cgatcgagcg gctcggggtc agggttgaca ttctgcgtgg
     3241 gcggacgcac agcgttttgg cgctcccgtg gtttgttgct gagatgattc gcatccgccg
     3301 ggacctgatg aggattgttg acgagcgccg aatcgatgtg atccagactc atctgctcag
     3361 agctctggac ttcctcgttc ttagccttcg acttcgaagg cggcttcgta tcttctggac
     3421 gattcataac accagattct cgcttcgtcc tgatcacttg catagagcac ggtggttgtt
     3481 acgacccaag caatggatgt atcggtggct ttaccgtttt ggatcgcgct gggtggacgg
     3541 aattgttgcc gtatcggatg aagtccaggc ctcattccag cgggaggtcg gcaactgcgc
     3601 cggcaagaca accgtaattt gcaactgcgt ggaagtagag cgctaccggt gcgccctgaa
     3661 cagggaaacc gtaagagcgc ggctcgggat cgccggtaat gcgtttgtta ttggagtggt
     3721 tgccacgttc aagacacaga aagggcacgc gtaccttctc gaggccgctg cctctctggt
     3781 tcgccggttt ccacaattgc atttcttatt cgtcggggac ggcgaactca gagaggatct
     3841 cctccgacgg actcgagctc ttgacgtaga ggcgcatgtc gatttcctcg gtattcgtaa
     3901 cgacgttccg gagctgctgg gtgcttgcga cgggttcgtg cttccatctc tttgggaagg
     3961 cctcccggtt gccctcctcg aggcgatggc cgccggtctg cccattatcg ccaccgcggt
     4021 ggatggcacg aagcaggcaa tggttcatgc ggagacgggt tggcttgtag cacccaggag
     4081 tgttagtgaa ctcgagcaag ccctgatcga acttctgacc gatcagccgc gtgcccgtgc
     4141 gatgggaact gcggcgcagc gaagagtgag tgaattgttc ggcaccgagc catacgtcgc
     4201 caagcatctc gcgctttatt gtcggcagca atatgcctga accaaacgga gtctgcatct
     4261 ggtttaccgg ccgtagcggt gctggaaagt ccacagtcgc tggagagctt gccatgctgc
     4321 tttcccgata tggaaggatc gttacaacgc tggatatcgt gccctgcttg gaaaaacgat
     4381 ggttcgaaag gaccagtgag ggcaagctca tccgaaaggc gtttgtcgcg gctgcgattg
     4441 tgcgtcatgg gggagtggcg atttgcgtga cggtaagcgc acggcaggag acccgagaag
     4501 cagttcgcga gctgatcggc gcctccagct tcgtggaggt cttcgtcgat acgccctccg
     4561 atgtgtgctt gatgcgaaga gccgtaagag ggcggagacc gtccgcctcc aagcgattca
     4621 aagccgcata cagaagactc ttggaagtta tccgtttctc gcgacgcttg tcctaccagc
     4681 cgtcactgtc tccggaagtt accatccacg gcgatagcca gactccttgc gaggctgcgc
     4741 gaacagtatt tcggtactta gtggagcggc gatttgtcgt gcctgacgac actcaaaact
     4801 gttcctcgag cgagtctccc aacggcgagt tgcaaacggt ttcgcagtca aggccatgac
     4861 cgcaaggact agagttctct gccctggggc acccggcgaa acagtccgtc cgttcgaaag
     4921 aggcccgata tttctggcag gtatcgaccg cagcgggatt ggcatcctct gcgagcttct
     4981 ggaatcgcac cccagcattg ccatgacacg gcgcaccaac ttctggtcgt tctttcttaa
     5041 ccgcttcggc gacctcagcc gccgcgacaa tctggaccgt tgcctcactg cgatgatgcg
     5101 ctacacgcgc acgcgggtat tcaggccgga ttcggaacag ctttatcgtt cccttctaga
     5161 tgagaagcca acctacccaa ggctatttgc gttgctcgaa gaaggacgcg cccgagaact
     5221 aggaaaggtt cgctggggtg ataagtcgat gaactcggaa gaatacgccg acaccatcct
     5281 gacagcatac cccggcgcca aaatgatcca cattattcgc gaccctcggg accggtacgc
     5341 atccttattg acgcaccgga gagttggtag agggggggtc gctgcgagca cggccctctg
     5401 gctgcgatcc gcacggctcg caaagcgcaa ctcactgaag tatccggacc gataccggat
     5461 cgttcgattc gaaaccgttc tttcacaacc ggaagcgacg ctacgtcgaa tctgtcattt
     5521 cctgggggag gagtactcgc cggccatgct cacggtggat ggtcccccgg tctccgccat
     5581 gccccagcct gatcctgacg gtgggcccac acctcgagtc gttacgactg cgtcgatcgg
     5641 caggtttcgc aatagtcttt ctccgagaga gattgcgttt attcagcggt gtactcacag
     5701 ggatatggct gaattcggct acaggcccga ggctgttcca ttgtcgcttt caggcagaat
     5761 cttgttctac ctcgccgact acccggtcaa catgactttg atgctctgct ccaagttgca
     5821 cgctgcgact ctgaagtggc tcggccgtac gccgtccgcc cgccgcatta tctctgaatg
     5881 cgcctcctcc ggtgcggccg ttcattacga aaggaccaaa gactcgtgaa caaagggcca
     5941 gtctttattg cgggtttaga acgaagcggg actaccctgc ttttctcgct gcttgcttct
     6001 caccctaaca tcgcaatgac gcgccgtaca aacttctgga agttcttctt taatcgtttt
     6061 ggcgacctag cccgtccaga gaacctccaa cgttgtatca ccgccatgat gcgatacgag
     6121 cggttggccg tgttgaacac cgattgggag cggctgcgca aggacttcca gcaaggcgaa
     6181 ccaacctacc cacgactgtt cgccctgctc gaaggacaac atgcaggtct ccttgggaaa
     6241 ccacgctggg gcgacaagtc tctgcgaacc gagcgctatg ctcagatcat cttccaatcg
     6301 tatccagccg ccagaatcgt gcatatcatt cgtgacccgc gggacaggta cgcttccgcc
     6361 tgcaaacaaa acagaatcgg cagaggcaaa gcagcttctg gaatcgcagc ttggctgtgg
     6421 tccgctcggc ttgcaaggca gaacctcatg aagtaccctc acgcctatcg tgttgtgagg
     6481 tttgaatcgt tggcatctcg gcccgaagac acgctgatgg acatatgccg attcattggc
     6541 gaagagtaca cgcctgccat gctgacgatg gagggcaccc cagcctatcg ggacaaaggg
     6601 gggaacagct cctttgaaaa gcaccggcca ggcagcattt cgacggctcc agtcggtcga
     6661 tttcgcaagg tgctctcacc ccgggatgta gcttttatcc aagcgttcgc ccgccgcgag
     6721 atgaccgcct ttgattacga gatggaaccc acacaccttg cattacgaga acgcttggct
     6781 cacagcattg tcgattggcc gatgaacctg gcgtacgtcg cagcctggca cgccctggac
     6841 gcatataaga ccagaacaac acttcagccg tcagcccgga gactggttca gtcagtttcc
     6901 tgagagctta ctgaaaatcc tggtggcaag tgcgccgacg taatccccgg gtctctgaag
     6961 cgaactacaa aacttgctcc gcactggacg ctatggaatc catgagtgca caactcgggc
     7021 gaatgacgac cgtacccttt tggttgagcc tttgcagcct ccatctactc gcttgcggat
     7081 ggagcctcac cgccctttgc tgtcgtgcgc cagaacctgt caggaacatg ccgcatcttg
     7141 cgcaggcgaa cggctgtcac ttcaccgttg gcccaggcgt ctcaactgtg gacggccaag
     7201 agaattatgc ggcgctgaag gctggcagcg tcgtctgcat ttcggcggga agccgcggac
     7261 cccttgtcct caagaacctg cacggggagt ccggaaaccc gatcaccttt gtcaactttg
     7321 gtggcgacgt cgagatcagc tcaagtggtt tcgcaggaat caggatcgca aatagcgagc
     7381 atattcgaat tactggcgcc ggggtatcgg gtcgttgtgg agctggtctt gcagcgtcag
     7441 agcagcagtg cggattaagg atcaccgctc ccgctggaag aggaatcgcc ggtgcgagcg
     7501 ccactcgcca tatcgagatt gaccacgtgg agattcacgc gacggcgtat tcggccataa
     7561 ccatcaacac aacctatgag aaagacggag tgaggcgagc gaacttcaca cagtacaata
     7621 ccttcctcca tcacaactat gcccacgaca tcggcaagga aggattctat atagggtcgt
     7681 cgcattacac ctccggtggt gacgatcctg ttctccgtgg tgttgacatc caccacaatc
     7741 tcgtggccga cactggatgg gatggaattc aggtcggctc cgcggtggaa gattgtgcta
     7801 tccatcacaa tagaatctat cgagacagtt tggcaaatgt tgaactacaa cgtagcggca
     7861 tcatgatcaa tcgtggcagc gtttgcgatg tatactcgaa cttcatcaaa gatggctttg
     7921 ccgcctatgg catttacgta cagggcaatg gtggtaaccg catttataac aatgtcgtag
     7981 tcaatcgtac aaaaaacagc ggtcgcggca tcacagtggc tacaggatcc aatgtcgatc
     8041 gcggtatctg cgtttcccac aatacccttg tgagcattga ggggctagga atcaacttta
     8101 agaacgcggc aggcgctggt aacttcatcg agagcaatct tctcgcagac gtgcgaggag
     8161 aacagatgta cgtgggcgtg gcgtctgcta cgcacaacag ttccttgacc agcgtcgagg
     8221 aagcgaagtt tgcggatccc tccggggacg actactccct tgcggttggt tcgcctatga
     8281 tcgatgcggc attgcccgtg tcgcggtgcg gtgttgtgga cgactacgcc ggaacactgc
     8341 ggccgcaagg agcagcccca gatatcggtg cgtacgagtt cagcgcggat tctgcaccca
     8401 cgcaaccgca cctccgatcg ctcaataatg gggtcttcat ggccgttgac cggtttccac
     8461 aatgacgtca atgccaatgt actcgcacac gaacactcgt gaaccggaat gtcagcctgg
     8521 ccccatcgcc cgcttgcgtt tagcctatgt cattggaaca taccccagtc tcaccacgac
     8581 gttcatcgat cgggaagtta acagcctccg tgaacagaat gcgggtccta cgatcatctc
     8641 aattcgccgt ccttccggcc ctctttcgca agatcaagag gcactgcgca gactcgtgac
     8701 ctaccttctg cctgttgaga tctcgcgctt cgtggccgcg catcttcact ttgccctctg
     8761 gcggccttct cggtactttg gcacgctgct atatctgctc ggacgacctc atcccggctc
     8821 tcatacccgc ctgcggacgt tcctccactt tgctgagggc gtagtggcgg cgcgtgttct
     8881 ggacaatgtt ggctgtaatc atcttcacgc tcactttgcc gatcgagccg ccacggtcgc
     8941 tctcgttgcg agccgtctct tgggagtcac ctacagcgtc acggcccacg ccaacgacat
     9001 ttatgtaaag ccgatcctgc tggcggagaa gatcgcagga gccgccttta ccgctacatg
     9061 taccgccttt aataagacct atctggaagg actcctcggc gacgaggccg ggcgcaagat
     9121 tcattgcatt tatcacggtc tcgacctgcg gaagtaccaa cccgccgtgt acgtcccggc
     9181 aggtcgaccc attttgcttt cggtcgggca gcttaaggaa aagaaaggct tctcttgtct
     9241 ccttaaagcc tgccagattc tgagggagtc gggtgcagac ttcgaatgct ggatcgtggg
     9301 tgaaggcccg cttcgtggcg cattgacaag gcagatcgac gagctcggct tgcgcgagat
     9361 tgtgcgcttg aagggttcgc ttccccatga agatgtcgtt gaatgctacc gtagagcgtc
     9421 gatgtttgtg cttccgtgta ttgtgggagg cgacggtgac cgggatggca ttcccaacgt
     9481 gattctggag gccatggcga tgcagttgcc tgtggtctcc acacgacact caggaatccc
     9541 ggaggccatc gaggacggcg tgagcgggct gcttgttcct ccgggcgatg acgttgcctt
     9601 ggccagtgcg atcggccgat tgctcgccaa ccctggggat acgaatcggc tggggagggc
     9661 agggcggata agcgtcacgg agcggttctc agtggacaga aacgtcaagt cgctgctcca
     9721 gctcttcttg ggccgcagtg agaccccaag ttcgctcgtt gggcaattga tagggaatta
     9781 ggatccggct atgacagaat gcgacggtcg ggttgtggtt gcattggcgc aaatgcaccc
     9841 gttgttcttg gtctggggat cgccctatcg tggcccccgc agtcttgttc ttgcacggga
     9901 gttaggaatc tcggatctgc actatgttta ctgcacatcg cgccaaggtc tactcagcgc
     9961 gctgttcaag tacccctatc aggccatccg aaccgcgtgg ctattgcttc gcaagcaccc
    10021 tgggctcgtg ttcgtccaga gtccgcccag tctggcagtt atcgtcgtgt acctgtattg
    10081 caggttaact ggcgcacgct atgtggtcga tgcgcatagc gccgcgtttc tctatgcagc
    10141 atggcgccgt ccggagtttc tttaccgtca tttggcccgc aaggccctgg ccaccatcgt
    10201 gaccaacgag catttccaga gtgtaatccg acactggggc gcccgagcct tcgtcctcag
    10261 ggacatcccg actgtattca gcacgcaggg gcagtactcc ctcaacggca gattcaacat
    10321 tgtggtggtg agcagtttct cccaagacga accccttaga gacattctca ccgcagccag
    10381 ccaactccct caggtgcact tctatatcag cggagacaag caatcggccc cacgggaact
    10441 gctcgattct gcccccgcaa acgttcattt cacgggattt ctgccaaatg accggtacta
    10501 ttccctgctg tcggcggctc aagccatcat gtctttgacc actcgaaaca acaccatgca
    10561 gcggggcgct tgtgaggctc tttccatcgg gaagccggtt attgtttccg attggcctct
    10621 gttgcgagac tatttttcgt cgggcgccgt acatgtccaa aacaccgaaa ccggaatcct
    10681 tgacggagta ttgacgctca agcgccaata cgaccgttac cttgccgaaa tcacacaact
    10741 tcgagcctca aggcaacgtg aatggcagga gaaaaagctc gccctcactc gcttaatcca
    10801 aagcactacc ctgtaagaca cccgcggcta ttgaaccgct ccacacgtag cggcctcaat
    10861 gcctcgcata attgacggcc gtgaacgcac atcatatcat tcgaccagac acggtaacag
    10921 cacagccctc ttctgctaca cacgcaccct ctggtcctaa catgagattc gatgttgtta
    10981 acacgctcac acgatcctct tggcacactt tcgtcgacaa caatcccgat gcacgcatct
    11041 tccattcgcc ggagatggcc gaggtctttg caaacacacg caacttcact cctaccttat
    11101 gggcggccct cgatcaaggc gccacagttc acgccctcat gctaccagtc gacatcacca
    11161 tactgccatg gcccctccgc gcaatcacca cccgcaccgt ggtctatgat ggagtactct
    11221 gctcgcccgg cgtcaccgga cagttggctc tcgctgccct actgcgccac tacaacgcgg
    11281 aaaggcagcc tcgcaccctc ttcacggagc tcagaaattc ctgcgacacc agcatgtttc
    11341 aatcaacact cgaacagaac ggctttctct acgaagagca catgaatttt tttgtcgatc
    11401 ttactcagcc aacgtcggtt ctttggaaaa acattcgttc gaatgcgcaa cgtaatatcc
    11461 gaaaagcaca aagacagaac gtcgtcatcg aagagggcca taacctccaa tctgttagtg
    11521 acgcctacgc tattctgagc acagtatata gatggcttcg tgtcccactg cccgatgtgt
    11581 ccctttttca gtcggcgttt agagttcttc atccggcgaa tctgatgcac attctcactg
    11641 cgaaggtcaa tggcgtcacc gttggagcgc tgacgctatt gctgtacaaa ggtactattc
    11701 tctattggta cacaggatcg cttcgagagt attcagagta ccgagtcagt gacttgcttg
    11761 tctggcacgc tctccagttg ggtcaactgc atcactgcca taccttcgac tttggtggag
    11821 ggggacgacc ggcagaagac tataccgttc gggactttaa ggcaaaattc ggcggacgag
    11881 aggtggcgtt tggccgcaat gtctgtgttc actctccgct gacgctgagg gcaggtaaaa
    11941 ttggctacag ttgtgtccga gccttactgt cgtagctgga cgctttaggt ggctctggcg
    12001 attttaaacg gaaagtgtga ggggagagag ggcgaagagt aatgagagcg ttatccgttg
    12061 cacggcaagg aaagagcact tttggcaccc tcaagcgtgt atcgaccatc cttgggcgat
    12121 atggcagcac ccctaccaga gttgccgagt cccttgagaa gtgtgtgcag actctagagg
    12181 agtttcactg tggggcgacg tttccaataa cggcctcgac acttgccgga aacgagagaa
    12241 tagttgagcg gtaccttaac aggggaatcg agttcgctgt ccacgggtat caacatcttg
    12301 atcacacgag gcttccgctc gaactacaac tggagaacat gtatagagcg cgccggctgt
    12361 ttgccgcggg tggcatgatc ccaagcgggt ttcgggcgcc ctatctccgc tgcaatgggg
    12421 acacgctcac cgccattgca gatgcgggct ttgtctatga tgcgagttgg gctctcgcgt
    12481 gggaggttac ccaggtagcc gagacccccg agtaccagcg ggcgctttcc ttttaccaca
    12541 gtgattcggc ggtggactat ccctcgttgc cccgttggta tggtgacgta gttcggattc
    12601 cctacaatct tcctgatgac gaggctctcg tggagcgtct cagcgtgaag accgaggagt
    12661 gtatgggccg gatttgggtc gatatcctcg acagaacctg gaagctcggg gagttgttct
    12721 gtttgggtct ccacccggaa agaacgaccc tttgccaatc ggcgctgcta gccacgttga
    12781 aacagacggt gtccaagaga cctcaggtgt ggctgaccac actcgaagaa atcgctacct
    12841 ggtggctcaa caggacgaga gccaaggtca cttgcactga aaagggcgac cggctagtcc
    12901 atgtcagtgt tcgcggccct aaaggcgttg tgattctgtc tcgcggcgtt caggtcgaag
    12961 cggaaatcgc tgagccttgg cttgggggaa ttcgtctttc tcaggccagc gaacttgatg
    13021 ttcgtgcctc ctgccggccc ttcatcggag tgactccgtc ttcggcgccg aggctgaaga
    13081 acttcctcag gcagcagggg tacatcgtcg aagagacaga ctgctcactg actcacgcgt
    13141 tcgtactcga ccgccctcgt ttcgacgaaa ccgacgaacg agcacttctg gagcagatcg
    13201 accagggcga tttccccttg gtctggcttg gccggtggcc taatggtgct cgcagcgcgc
    13261 tatgcgtgac tggcgatctt gatgcgttca cactctggga ctatgccagc cggctgctac
    13321 cggctcgact gtcgacctac tttctcaacg agcatcgatt agtgatgacg cctcgctcca
    13381 ttcggtcccg cggtgttacg tcatgaggga cgccctcgat ttggattaag agtgggaagc
    13441 atcaaacatg gctagatatc tcgtcacagg agtagcgggt ttcatcgcct ccaaggtggc
    13501 ttctatgctc gtgggccagg gccacgaagt cgttggcttc gacgatctca acgacgctta
    13561 cgacgtccgg gtgaagcgct ggcgactcga ccaattgctg acactcgatg gttttcactt
    13621 tgaggagttg gacattcgtg accggcgtgg tctcgaggag acatgggcca agctgccgtc
    13681 attcgacgct gtcgtcaacc ttgctgcacg ggcaggagtg cgacaaagcg tgtcaaatcc
    13741 ttgggtttac tttgaggtaa atgcgacagg cacattgaac ctgttggagt tgtgccgagt
    13801 tcacggcgta acgaacttca ttctggcttc gacgtccagc ctgtacggag ggctgaaccc
    13861 cgtcccatat tccgaagaga cggagaccga tcatccattg tcaccctacg ccgcctcgaa
    13921 gaaagcggcc gaaacgatgt gctttacgta ccactacctt tacggcctgg acgtcactgt
    13981 ttttcgttac tttacggttt atggaccggc cggaaggccc gacatgagta tgtttcggtt
    14041 cattcagtgg attgcggaac aacggagtgt cgtcgtctat ggagacggga cgcaatccag
    14101 agactttacc tatgtggatg acatcgcacg gggcactatc gccgctttag gacgcaaggg
    14161 atattctgtc gtaaatctcg gctccgacca cccattagtg ctgatggacg tccttcgaat
    14221 gatcgaacag cttcttggaa tttccgcacg gattgaacat cggcctgcgc atcgcgctga
    14281 cgttcaggca acctgggctg acatacgaag ggctcgggcc acgctggggt ggtcgcccgc
    14341 ggtcagcccg cagaagggca tcgagaacac ggtggagtgg tacctcgaaa atcgccactg
    14401 ggcaaaggac atccagacca ccgacctcta gcacacgccg agccttgctc tcgattcttt
    14461 cgcacgccgg ccctggacat acgtgttttc cgctttcagg agagtctagc tcacgatgta
    14521 tgacgaattc ttcgggctgt cctgcatgcc cttcggcacg tcggcggacc tgaaattctt
    14581 gtacctcacg ccgcaacatc gggaggcggc cacaggcctg atctgcacca tcctgaatcg
    14641 agagggctgc gcccttctga cgggagaacc tggcacaggg aagacgatgg tgttgaaggc
    14701 tacgctcact tcatttccgg ttgagtcggt gattatttgc gacttattaa tcccgacctt
    14761 gtcacaaacg gagctcttgg agtatgtgat gttgcgtctt ggcatcgacg ctgcgggacc
    14821 ggcaaacaag gcgcagcagc tcctgagcct tgaaaggttc cttctcgacg ccagatatca
    14881 gggccggacc gtggtgatgg ttgtcgacga ggcccaaagt cttccggtgg acgtgctgga
    14941 agagatcagg ctgctgatga accttgagga ccacgaagga aagcttctgc aaattgtgct
    15001 agccggtcag cccgagcttg acgccctcct ccggcagaag gcttcgcgtc aattcaagca
    15061 gagagtggcg tatcgcttct cgctgcgccc gctttcaatg ctggatgttg agagctacat
    15121 tgatcaccgc tggcagagag cgggagggac cgcgcggcct ccttttgagc gagaggcgat
    15181 tcgcagcatc gctgcatatt cgcggggaat ccctcgactc atcaacataa tttgtaataa
    15241 tgcgctgcta gcggcgtttg cctgcggaag cactttgatc cgtgcctctc atgtcgtggg
    15301 cgccgctcag gacctggacc ttattgaacg aggcgaagtc gccgcaccaa gagccgccgg
    15361 cactgaggat gagatcgccg acttgcagag actgacctca ggaagctccc catcaaggcg
    15421 aagcaccgcc cggtccgccg ccgtgtccgc gggccgtgcg cctgcatcac cctggctggt
    15481 gcgctcggca aagaagttgc tgttgcttcg atagctctct ctcgcttttg aacttcggtt
    15541 gtgaaaactg caaaccgata tgaaatctaa tacttcgcca tgccactgta tggtcgttca
    15601 cgggcgctac cccatcactg agacacgggt agaacgcgag gcccgggcgc tggtcagccg
    15661 gggatattcc gttgacgtga tttgtctgcg atatgaaggt gaatcggcaa cggccatcga
    15721 ggataacgtg cgtgtgtacc gcctcccggt gaagcgtcac cgggacagcg gggtatgggt
    15781 gcaattctgg gagtacctcg cgtttttcgc gctggccttc ctcaaactca gcctgctgga
    15841 ccgtacccgc cgctacaaga ctgtgcaggt acacaatctc ccggattttc tcgtgtttgc
    15901 cgccctggta ccgaagctgc ggggagcgaa ggtggttctg gacttgcacg atttgatgcc
    15961 ggagttctat gcggcgcggt tccgacgcag cctctgcagc cttcccgtcc ggctgattcg
    16021 ccttcaagag cactggtcgt gccgtttcgc cgaccatgtg atcaccgtca ctgagttgtg
    16081 gcggcggact ttgctggacc ggggcgtacc cagtcgaaag gtgtcggttg tgatgaacgt
    16141 ggccgactcc aacatcttca accgcaacgc ccaccgggcg ctgccgcgga cctccggcga
    16201 attccgcatg atttatcacg gtaatctcac acaccgttat ggagtagacc tggctgtacg
    16261 cgcagtcgct cgcatcagga aacaggtccc gaatattcac ctcttgatcc acggcggcgg
    16321 tcctcttctt gcggcgctgc gccgtcaggc tgcggacctc ggtttggacg gggtgatcac
    16381 cttcagcacc acacaagttc cgacacccga attgccttcg ctgattgaca ccgccgatat
    16441 cggcatcgtg ccttaccgtc ccgacgtgtt caccgacggc attctgccca caaaactgat
    16501 ggagtacgtt gccatgggct tgcccgtgat tgccgcgcgg acggcctgta ttgcgtccta
    16561 tttcgacgag gacatggtga cgttttttcc cccgggtgat gacgaggctc tcgctgactg
    16621 catcagaaag ttgtacgacg atccagttcg ccgcgcacta atggtggagc aatcagaggc
    16681 gtttctacgt ctgcacaatt gggacaccga aagcgcggca tatgtagcgt tagtcgaccg
    16741 gctacagacc agttcccgtg gaccgattga ggtggccgag gtcggaagcg catgaatgat
    16801 tcttaaggaa gtgatgacgg cggcgtgtcc cttcaaactt cgagccgttg tcagagtgaa
    16861 aatatgtcta gcggctcaaa tggtcggcgt gcaatgtcct cgggacttcc gtttcaggct
    16921 ccgccttgca ttggaagcct tgactccggg tgtgagtttt gtgcatactg ttcgccaaga
    16981 gcccccccga gacatctgat tatggcttct gtcaagagct gctgagtaaa gaacggctca
    17041 cgaacggagg ttgcaaggag cacagcgttt cgcaggaggt ctcatgccaa gctcgaaagt
    17101 gttggccggc aacgagatgg cggtcggttt cggagagcgc cgcagatcga cgcgatttca
    17161 gattgaaaga acagttcgct gcaagtcgga aactggggcg acccgatatg gtagaaccgt
    17221 cgacattagc ggcagcgggg tatcgttcac cactgaagat ccaccgattc ttgggcaatc
    17281 ggtcgaactc actgttgact ggccggttcg gctcagaggc gagacgcatt tgaaactcgt
    17341 cattctcggg caagtggttc gccgcgagcc gaatagcgcg gcagtcgcta tcacgaatca
    17401 cgagttccgt actctcggtt cagacacgtt ttttgcgctg accggctctt aagtgttgaa
    17461 ttcctgagtc ttcacgatct aggcggatga gtccgcaaaa ggagccgttg tgcaaaatga
    17521 ggaatgcggt cctgagctag atccgcttct acccgaactc atcagcatcc gggaaagcat
    17581 cagggcgctg gaggaacgga agctggcaat agaagagcag atcctgacga gcctctttgg
    17641 atcccccagc ggagttgtgg aggagccgcc cgagacctcc gaggaaacac tcgccgaatc
    17701 tccctcgctc gctgaaaagc tggctcctcg acttgtgacg ccggctgaca gggagcgcgc
    17761 cgcgatccgg atttccggca tcctgatgga agcattggtg cagtacttcg cggccatcga
    17821 agagcagact ctcagcgaga gccgcaaaac gactgcctcg ctgcggcagg agctggaaga
    17881 actgcggtac aaccttgaaa gtccaactgg gtcacttgcc actcagctta aacagcttca
    17941 agaggagctg tcgtccttgc agagactgat caacggcgcg gtggagcggc tcaatcggca
    18001 agcagaagcg atccgctcag tcacagaagt gcaggggcgg catgccagtg ctctacggca
    18061 actggtagag actctcatac gttcagccat cacatcagac atccctccct cgcaagcaaa
    18121 cacccaactc taggccgatt ccagagttcg cggcgcccct ctccctcttt tccgtggcta
    18181 acctaacctc agcgagaggt agtgtcgaca cgcgcaaggg atcccgtggc tacggtccta
    18241 ctttaagaag cggagcggcc agatagattg aggacacggc gaagagaact actgctagaa
    18301 gcaccttcag ccagaaggcc ggaacgaatc gttgcacgcg ggcgccgaga tacatgccgg
    18361 cgaaaccgcc ggatccgaaa agaccgccaa gcatccagtc gggagcgacc gccacaccgt
    18421 tactctggta cagaggtgcg atcaattccg caaaggtcac cccgaccacc gaagtgagga
    18481 aggttcccat cagcgtcgcg ccggccacgg tatgaaccga cagcccgtac agggccacga
    18541 aaaatggcgc gaggattgct cctccgccaa tcccatagac gccaccgatc aggcccacga
    18601 ccagagatac cagtagcact ccaagcgtac cgcaacgata ttgccggcct tggaactcgt
    18661 agacgaggtg acaccggtca aaggccacct tccggacagc ccagtccgcg ccatcggaaa
    18721 gggccagttc cccctcccag cccgcatcgc gcctgacgcg ccgtgcatcg aagatgaggc
    18781 ggatccctat agacagtagc acgcacgcga cgaatatctt gaagcggcca ggatccggca
    18841 gccagcttgc tctcatccac tcgcccacca caactccagg tgcaccaccc gccacgacga
    18901 tccaggccag cggtgcaacc agtcgccgct ccctccaata acgatagatt ccactcggaa
    18961 tggcgacaat gttaaagaca agattcgtcg gactcaccgc tgggctgaca aaaccgagaa
    19021 cactcatctg gaacggcaat agcagaaccg ctccggacac tccggccatc gacgtgaaga
    19081 aggaagccac aaagccggcg accaatggca cccagacagg agcttcgact cccgacaccg
    19141 ggaaaagggt taccatcgcg tctcagagta tcagacccat ggaagggcag aggcaaagca
    19201 gtccgtttat gaacgtcgcc cgcgtgggca tgcgataaag agcagaccgt cggaaactcc
    19261 tgattttgac ccggcccgct aagagacggc atcatggaac agaggtgatt gatgagcaaa
    19321 ctcgtggccc cacatggaag cccggcgttg aaagcgctgc tagtggaagg taaggagaga
    19381 caggaagcac tggaaaaggc gaaacgcctg aagaaggtcc cgatgacgag tcgcgaatcc
    19441 agcgacttga tcatgatggg catcggtgca ttcacgcccc tggaaggttt catggggcgc
    19501 gacgactgga agggctgctg cgacgactac aagttgccct ccaagagcgg cctgttctgg
    19561 cccattccga tcacgctatc ggcctcgcag gatgacgcca aggcggtcgc gattggcgag
    19621 gatgtcgccc tgtgggacac cgagacctca acgatcatgg gcaccatgac ggttgccgag
    19681 aagtacacga tcgacaagga gcacgagtgc aagcaggtgt tccgcaccac cgaccatgcc
    19741 catccgggcg tgaagaaggt gatggagcaa ggtccggtga acctggccgg cccggtgaca
    19801 gcgctctcgg aatcctacta tcccgacatg ttcgccggca tctatcagcg gcccgccgaa
    19861 gcgcgtatgt tgtttgaaga acgtggatgg aacaccgtgg cagcactaca gttgcggaac
    19921 ccgatgcacg gctcgcatgc ataccttgct tgggtcgcca ttgaggtgtg cgacggcgtc
    19981 tacatccacc aactggtcgg gaaactcaag ccaggggaca tcccggcaga agtgcgagtt
    20041 aaggcgatcg acgccttggt gagcaagtac tttcgtaagg accgcgtcgt gcaaggcggc
    20101 tatccgatgg agatgcgtta cgccggaccg cgcgaggcgc tgctccatgc cgtgttccgc
    20161 cagaattatg ggtgttccca cctgatcgtt ggacgcgacc acgcaggcgt cggagattac
    20221 tatggcccat tcgacgcgca gaaggtattc aacgagatcc caccggacgc tttggcgcta
    20281 cgtccccttt gcatggactg gacgttctac tgctacgaat gcggcagcat ggcttctatg
    20341 aagacgtgtc cgcacgagag caaggcggtg atcgatgaga acggccaata cagaggcggc
    20401 gcgcgcctgc tgctttcggg cacgatgctg aggaagctta tgagcgaggg caagccggta
    20461 ccatccgagt tttccaagcc tgaagtgatc gaaattctga aggagtacta cgatagcctc
    20521 gaagagaagg tcgaagtaaa gttgcatggc ttcgccacgg gagattcggg aaggaagtag
    20581 cctccgccgg ccttcttggc gcttggcaca gaatggagga atctcagtcg ccggcttcgt
    20641 tcagcatccc ggagaagatg ttgaagaacg taacgccgtt cgagccgtcg ggatcggcga
    20701 tatccgctgt cgcaggcagg accgtagtat ccgcggggcc cgcgtacagt gattgaatcc
    20761 gtcccaaccc gccttgggcg agcagatcgg cgaccagatc gaacgccgag gccggacggc
    20821 ccaggcgcag ccagtcgccc gtgggtgcgg ctccggcgac gggagcggct acgcgaagct
    20881 tcgcggccat gagcacgcga cggtattctc gaacgttcct cgagtatccg tgctcgaact
    20941 ggcggtcgaa ggatgcgatg gcggagctgc cgtcgaacgt cattccgcgt ctgcggaaat
    21001 gtgtcgagcc caccaggcac cacacgtcat ccacaatcac ggaagtggtg cggatgaacg
    21061 ccgtccggcc cgggaatccg accggatgaa acagcgccac gcggtcgggc gcggcggcga
    21121 ggaggttgcc gagagcctcg gagcgagcct gatagtgctg tctccaccag cctttgaagt
    21181 tggtggcgaa gtctggctcc ttgggcgtgc agatcaagac cttgagattc ggcgcttggg
    21241 aaagacgcgt ggcgagctcg gccacaagat ctacttcaaa aggctgaggc ggtccagcgg
    21301 ggcgtgcggt gcgggcaaac tgcggtgatt cgatgtacac cagctcacgc gcctcggcga
    21361 tggcgcggcg cagcgaatac tgcgcgtctc gcagtccgtg cttggagacg aagaactcgc
    21421 ggcggacctc gcgctgcacg cgcggctcat tctggatggt gatggtaatg ggtggcgggg
    21481 gcaggcccat cgccgccgct ccgtcgttga tcatctgctg cacggtgttc cccggcggag
    21541 gcggcgtgag gctgctgagc cagggtgttt cacatcccac ggcgaccgtg tcgagcagca
    21601 ctccgatgcc ggtggcgacg ctggcggaat cgtccggcac gttgaaattg tctcccagtg
    21661 aggcaaccac ccatcccgga ttcgctccca cggttccggg aagagggatg atcggctgcg
    21721 cccgccgcag tgccagccga gccaagtcgt agccgagcgc gccagtcact cgcacgcccg
    21781 acgcgtgtac gtcggggccg gcgggattgc ctgggtttcc gctttcgtgc agcgcggatc
    21841 gcgattcgcc ggataggcgc ccgccgctca gcactccgtc ccacaagagg ctacctgcgg
    21901 gagttcctcc ggtcgcgccc gccaccacca ccgtgtcgaa gcgggccatg gtgggcaggc
    21961 ggggagcctg gcggggcgag ggctcggcag ccagactgag cacgagttcc agaaaattcg
    22021 agggtggagg tcctgccgga ggaggtgcgg tcttgggaat tccgaacagc ggcgattcgg
    22081 cgacgccctc cgcctgcgga agcatcacgc tcagaatcgt agtaccaggc gtggctgcaa
    22141 agggatcggc cggcgcagcc acaggtccgg cggcgacatt ggcagtgacc gcgccaaaca
    22201 actggcgcct tcccacgcgg ggcgcgatca cgatgtccat tgtcagcacc gccggactcg
    22261 gcggaggctg tccggacgcg agccggaatg gattccgcag gaaaacctga accgcgccgc
    22321 ccgtcgaaat agacgcgcct ccatcgcctc gcaagaacga gggctcctca gctatggcag
    22381 cgatttcgac gaactgctgc ggatagatgc ggatgtgcgc gccattcggc gcaatacctg
    22441 ccgcgatggt ggcgacgacg tcattagccg cggtgaaggc ggcggtgacg gaaccggcag
    22501 gcagcgcggg cggtgcagcg aatcccaggt tcgtgttggg cgcgggaaac gcgggccagt
    22561 ggccgttcgg cccgcgctga ggcggcgtct gaagtgtcgc gtcgatgaac gacgacaccg
    22621 ccagcaccat tgcctgggac ggcagattga gcaccgcgtt cgcctccgcg agcgtgtccg
    22681 gcccatccgc aagataatcg atcgttacgc ggtcgcgaac ctgtggcaaa agatcggccg
    22741 gaatgcgctg atcatccgcg ggcacaccca gcacggcggc gctggtgcgg ttgccaagca
    22801 acatccaatc cacgtccgct accaccacgc ggaagaactg ctttggcagc gcgggcgcgg
    22861 gcgccggacc gcccggaagc gcgggcggaa tcaacggagc gcgtcccatt gtgccgaatg
    22921 tcccccagcc ccagcgcaga cggccgccat cggtgtttgc accttggatt cgatctccag
    22981 cagcaagcgt tatcagaccg ctggcgggta ccgtgccggt aacgttcgac cccgcatccg
    23041 ccgtaaccat cgtcgcgccg gcaagggcgg gccggtacaa gccaccgtga ggatccacga
    23101 agtgcagcag cgttcccgcc cctccaatcg cgccgatctg gttcacacct cccgcgccag
    23161 ttgcaggccc gctgacagtt gtaggctgca gcccagggag tgcgttcagc aggtcatcga
    23221 acagacacgc cacgtagatg ggatcgatga tctggccgcg gccgtcgtga aacgagatgg
    23281 cgccgctgat gcctgtcatc acctcatctg gttcgaacca gcgcgcggta ctgaagcctt
    23341 ccacgccgcg cacgaccatc gcccacggga ccggccggat cggcggatcg ccaggctgtg
    23401 cgtaacgggc gaaggccagg cgttccagcc ggaggagcgc ctgcgggtgt aaccgcaaga
    23461 cggtgcacgc tcccgtgaga ggtatcccat cggctccgta gaattccgtc gtgcgagtgc
    23521 cggccatcag gcggagtgtg accttgtcgc cgggcgagaa gctctgcttg gcgcttccat
    23581 cgaggccgcg cgaaacggtg agcgagtcgc ccgtgcgcgc tgtgcatcca accacctcgc
    23641 gcttggagcc cgaggcgttg gaaaggacca agaacaccga tccgccggtg ggtgagggaa
    23701 aatttgcgcc agtgccggcg gctagcgtga tcgtgttgtc gttttccgat gcggcgttcg
    23761 ccagcgtgga actgacgggc gtgaacgcgt gctcacgaat tccgagaaat ggcgctcgga
    23821 tgtcgccggt aatcattaag gtcagcgccg cggagtcata gtcgccgccg gctggagtag
    23881 tgttggccca gcgaggactc agccgaaccg tggtcagtcc tagcgcgccc aggccgttgt
    23941 tctgcagatt ctgctggact gccgcgggcg tgagcgtcgt gatggggaaa gtagccatgg
    24001 cgtcaaatca ctggttggac gtgctcggca aaatgaccgt ccggcgaagt cagccgcacc
    24061 acgaactgct tgacaggaac gcggcagaac gcatagtagg agagtttcgg cccggcgccg
    24121 gaattgcggc gcacgcgcag gggatccact cccggcggca cagggccggg ctcatctaat
    24181 gcaatgtcgc ccaagcccag ctcaatggtg atcggaggct gcggaatgaa gggaccgata
    24241 ggcggaaaga tctgctttgg cggacggatg gccgtgacgc gcaggatgta ggatcctgcg
    24301 tcacctgcgt ccagcggggc atcagtgaac cagctcagca tggttcctgg gggcgccacg
    24361 ctcgcggtca ggacgaagcc ttccaaaacc ggcagtggca gcaccgagcc cggcttgatg
    24421 gtcgtgcgct gctccgagct gcggcccaag gggtccgtga tgcgcgcgga gaactggacg
    24481 gcatcgttta cgtcggcacg gcggataatg gcccggtatt gtattgggtt gggaggcgcg
    24541 ccacccgctc gccatgctcc ggaaccggtc ccaggctgcg tgacgcccag gctttcgagc
    24601 ggcccgtcga cggaaatcag cggaagcgtt cccgccggtg cgcccacgac cgccgcgcga
    24661 atcgaaatcg tgtgcgggcc gagcggtgtt ttcttgaccg gcaccgggct agcccactta
    24721 atgaggacgt cacccggccc tccgccggag ggccattcga cttgcagggc ggagatcacc
    24781 ggcggatcca cgggtgggac cacgacccag ctcacgctgc tggcaggcga cttacccgcc
    24841 aggttgcctc ggaggtcgtc tttctcagac catgcggtgg cgcgatacca cactcgtttc
    24901 cacgagccca gcggtgtatc gaatccctgc gcgacggtga tatgcgagcc gagcgtatcg
    24961 gtttcctgct gcaccgtcca acccgcgctc gcgacgttga gcgtctgcac gggcggcccc
    25021 atggtttcga gctgcttggc tgcgtcatcc acgcgcacgc gatggagatc gagccgccgg
    25081 acgcgtgggc ctttgcgcgt gccgatgcga atgcgggcgc ggtagacgtt tgggatgacg
    25141 gattgatcga ggaacgactg tgcctcaatg gtgggagccg cgggcgcgag cacgcgcggc
    25201 gcggcaaagg cttgcagcat gtcgtcggcg ttcgggccgc tcggccagtt ggcctccact
    25261 tgccctgcac taacgcccag caccaaaaac acgtggatcg cggtcgaacc gcgcggcaga
    25321 gtcacatcca tgctggtgcc ggtgaccaat ttggcgttgc gccgcgtgaa ctcccgccgc
    25381 gatggattcg ccttgaagcc gttcttgatg cgcgtgagcc gctggctcaa cgtcaagtcc
    25441 tgcgcaggct cgctaaggtt gttagccagg agaattttcg tctccgtcga ttcgtaaacg
    25501 aagtacccgg ttgcgccggg agttgcggac cacgccaggc gggcgtggca ctctcctgag
    25561 gcatccggaa ggctggccag tgtcacgata tcgggcggca caaaaggcgg ccttggatcg
    25621 gacacggaga ttacgcttgg ctcggcggac caagctcctg tgcgctgcgg cgcaatcctt
    25681 tcctgtccct gcgcccacag cgcgaggccg atgtggcccg tggcggcgta attcaggctg
    25741 aagccgggga tggtaatgcg gtagcgccgc gtctccgatc cttgcggcgg cccaaacgag
    25801 acggccgtat caccggcctg gttgagcccc tggagtgttc ccgccgttga cgatggaacg
    25861 tctccggcga acgtgatctc tgccaccgga tcagcgccac cgagtgaacg ctgcaagccc
    25921 gagggcacgg tgacaatcgg cggaggatcg ccgtggaacg ctgccgcata cagcctcccc
    25981 gcaaagcgaa tcgtctgcgg ccggcggatg cgccagtccc acagaaactc gatgaccagc
    26041 gtgccaggac ataccgtccc ggacgcaggt accgaagcgt gcatttgagc gctgacgatt
    26101 ctcacctggt ccacggccgg ctgagagaca gtgcgattga tcgcactcca ggtgctccac
    26161 tggccataga tgtcctggtg cgcggccgcg tatttcatcg ttcggtttcc aggattggat
    26221 gggatcggca gttcgcggtg aaccgcgtgg atgcgccaat tctccggatc aggtggcgtg
    26281 atcgtctcgt tgatggcaat tggcaggaaa ccaccgcttc ggcgctttgc cattactgcg
    26341 tctgtgggtt ccgcgggagc gacaccggca cgcgcgaagg cgtatgttcg cgggcggaaa
    26401 agaggaatcg caggcggccg gtcccacccc acgcgcactg aacacagcca atcgaaatcg
    26461 gtttgtctcg ggcgcagatg gcccatgaac ctggtcatca tgtttgcggg cgcgggaggc
    26521 ggaatggcct gctgcggagt ggggaccagc gccgcgtatt cgaccggcgc ggagttgccg
    26581 tcgaagcctt tatcccagcg cgccgtgatc atgtagtcgt attgcgcggc attcggaata
    26641 ttgacggccg caaacgactg cgtggtgacc gggtaagcgg ttccgaaccc gaggcccagg
    26701 ctgaagtaag gatctgtgcc cgcgccaatc agagttagtc cgagaggcga gacctttgat
    26761 gttccccccg cgacgggcat cgactgtccc gatgagtttt ccgggggcgg aagcggaacg
    26821 ttgagttgct gttcgtagtg cgcattggga gggaaattgg cgaccaccgg ccgcagaaat
    26881 tgcagcagat cggtattcag atccttgatg agatcggtga agtccggtgc gctccagagc
    26941 ggcgcgggta cacccgtatc gagcagcggt ccccatccga ccggaggtgc tccacgctcc
    27001 aggcgctgcc gcgctgcgtt taccggagac gtgagcgcgg cgaccatgcc ttggttatcc
    27061 gcgtgcgtcc cgacgccgga ccattcgggc ttggagacgg gcaaccccac cagctcgaat
    27121 ttttcccagc ccgcggcggc ggagagatcg tcagcgggga tgccgcgcac ttgcaggaca
    27181 ttgatgcccg cagagacgat cagcccgtcc atggctgaag cgcttaactg aagtgttgcc
    27241 ggaccgccgg gagctgaggt gaaggcgacc acttgagaaa gaagtggcgc tccggcgaac
    27301 gccatcacca ggccggccgc accgttcaca tcgatttcga ccgtagagac cgtgtcatgc
    27361 caatcgacca gccgcgtgcc gaagaagaag tcgagcgagc cgacgtcgat ggcgagaggc
    27421 acaggcgcgc gcgcctgccg caaccgccgc cacacagtga aaggcccgct gggaaatccc
    27481 atgtcgcggt ggatcatcca acgcaactcg agcaccgcac cgagccgggc cgcttccgta
    27541 cgcgcgacat cgagcagtcc gagccgccgc gcgacgaccg cccaattgag gaaggcgcct
    27601 tcagcctgaa aacgagtggt gtcgacgatg aaggccatct cagtcctctt cctcccacgg
    27661 ggctttgaca aaaatgaagt ccgcgacgct gaggaactgg tccgttgcgg cggggccgtc
    27721 aagataaggt tccttctgcg cgatacgttt gagcgccagg cggatgtgct tgccgcgcga
    27781 gttcggcttg agcgtgatta gagcgcgctg ccctcccggt gcgcgtacga ttcgatccac
    27841 gacaggatcg ccgccggcct gctcggagag cgccagccat tccaccggcg tgagttcgta
    27901 gcgtttgttg gcggcgggac ccgggtcggt gatctcgcgc ggaatcgagc gcgtccgcca
    27961 catcggttcg gatgcgtcga tcaacacagc cgcgggctgc ggcttgggta caccttcctc
    28021 ccagaagacc accacgcgtg ggaatttggg aacaggaagt ggctcgaatc cggcagcagt
    28081 gaattcctga tcgagttcgc tgcctttggg cgccgaggtg gcgaatttca ctccgatcgc
    28141 ctgcaacgca cccgtggctg caaaccggtg ctggacagcg gcgatgccga aggattccgc
    28201 aaacgcagcc gttgtcgcga aaccgcccgt gctgaacgaa cgccgccaca caaattcgcc
    28261 cacggtcccc tcaggcgcgg ccttgtcgac gcactcgata tcgaggacgt aatcggtgaa
    28321 cggctcgagc gggatgggga cagtgaccat ggaatggcgt gtccgcgagc cgtcgacggg
    28381 cacgcacgta tcggtgacca ggcccacaac cgcgtcttcc cagggtgaaa gcacatcggc
    28441 cttcaccggt tgaagcgtga catcattaat cgggaacggg tgcgggacag caggtgagga
    28501 cggagttggc cggaacgacg ctgccttgat gcgcacctgc aagcgcctgt tgtaagcgtc
    28561 gtagatccgc gcgacgttgt tggtgccgaa cacgatcttg atgacttccg cgccgaagta
    28621 ttgcttttcg ctgtcttccg gcgtgctgca cagcatccac ggatccagcc ggcgcggcgc
    28681 ttcggcgtcc gtcttgaacc agaacttctg gacttggttg gatacgtcct tgtgatccgt
    28741 ggctggctgg cccgcggggc gccgctcgcg cgacgcatcc cacttgacgt ggactccgta
    28801 aacggtgttg ggcagcagca acgcctggtc cgcgctttcc gggccgagcg ctgtttcgag
    28861 cacacccttc ttcgtttcct gctcgagcgt gtcgtagtcg aaacgcagcg tttcaccgta
    28921 gcgcagagct tcaagtccaa ctacatagaa aggtcgcttg gtgaatgcgc ggtgccactt
    28981 gggatcctgc ggccggagtc cgacatggat cgtggcggtg tcaggatttc ccttcaccat
    29041 gaccaccact gcggagtacc cttgcacctg cgacagtgtc tggaattgca ccagaagttg
    29101 catatcatcg cgccacggcc ctgctgggtc cgtccagcgc gccgggaaca tattgggcgg
    29161 aacgcgatcc gccggcgtca ccagccgctg cgagaggaca ttctcattcg cgtcacattc
    29221 cgcgatcatc agcatctggc cttccagcac gcggccaggt accagcagat agaacgttgc
    29281 gtattcgacg cgacccgtgt ggaagactac ggcgtcatcg aagggccccg gcttgaactt
    29341 cagcttcttc cagccgtccc ggatctgctt ggcgcgctgc ggatcgccga acggctgcgg
    29401 tttgcgcgaa tcgaacacgg gcgcagccag cgcgcgcgta aagcagcgct tctcaccggt
    29461 tggcacggtc gcgcccgcgg tgcccgcagc gccggcttgg ggttcgatgc ctcctgcgac
    29521 caatagcgcg agtgatgaac gcgagatggg gataccgttg ttgatccggc ggaccgcttc
    29581 aacgagattg atcctctctt cggagacgac ttcggtggac ttgatgcccc gcgcagttcc
    29641 gacgacgtcg agcacagggc gcgccgccgc tgacgtcagc gcggttgttg cgatcgccgg
    29701 agtgagcgcc tcggcaagca ccggaactgc ggtcgtggtc accggtgcca cgacgggtgc
    29761 ggtagtagac ggccgggtgt cgggccgcct cgacggacag tgcgctgcgg cgccttcgac
    29821 ctgagcggga atgattccgg tgagcacgtc gagcttggag tcgccgcaac gccagcgctc
    29881 cgtcaccttg agcaggatgt ccgggtcttg agaacgcacg gtgttgggcg gattgggcca
    29941 agcctcgcct tcgaggatcc agccgatggg cgagggcccg agcacttcgc gaaggaagtc
    30001 ccagaacacc ggcgcttgcg gagcggcggg ccagcacacg gtaccccaag tttccttcac
    30061 ggtctcttcg aggaacttgc tactttcgat ggccttgggc gtggcttccg gcacccagct
    30121 caagagtgcc agttgagctt ccagcggttt gtcgccggat ttctgtgccc accaagtggc
    30181 gggtgtggcg cctgccgtca ccgagccaat gaggtcgact tcggtcagcg tgtatttgaa
    30241 gaagacatcg ccgcgtggga cccagccgtc gggaggagcc tccggcgtgc cgttgaggtt
    30301 ctgtccttcg aactgcactc cggcggctgc gagaggcggc gtggacatca tgagcagcgg
    30361 aatcgcgtcg attggcacga ctggaaggcc accggcgggc gccgcgcctg cttcgacgcc
    30421 gtccccgatt ccgccgtcga tcgacttgtc cgttccggta ccgacgacga gtgcgggaga
    30481 gcgactgatc agcttcaggc tcttcaccag cggcggtggg tccggcacag gcacgctcgg
    30541 atcacccagc gcaaaatcca cgcagccttc gatgctgaag aacagaaact cgatgcgacc
    30601 gcagatttcg ccttcgatac gcgagatctt gttgtgcgcg gcgtcttcgc cgacgtcgac
    30661 tttgagcttg gcccacgcgc tgatgtcgat gatgaacagg tggagagttc cgcgaacgta
    30721 gagaatgccc gccagacgga agggatcgaa gccgacgatc gcgtcgaacc cggcagccag
    30781 ttccgcgtaa aggccggctg acttgctccc ccacacgaac gagacatgca gcccagtcgc
    30841 gatggagaat cccgtgacgg ccggcaagtc cgggattccg gaaatgccgt tgccagagag
    30901 catgagatag ccgctgccct cgaagacctc gaggatctta gcgtggatct gatccgaata
    30961 gcgtcccagg aagatatgcc agtctttggt gtcgttgaaa ttaaagaacg cctcaaccgg
    31021 aatgcggatt tccaacagcg gactgacttt gtactctact gaaatgccga tggtgagtgt
    31081 gccacggccc atgtcaaggt cgatgacagc cagcagcaac ccttcggcgg tgccgcccag
    31141 ctccggcatc accttcagca ggtttgcctt catcatcagc agaaggcgcg gtccaggcag
    31201 ttcgagcaga ataacgccct tcaggttaaa gatgatgcta ctgcccatgg tgcctaggat
    31261 ggcgccgacg ccgaaggccc acgtgttgac acgaggcttc cagaaatcga tcttggtggg
    31321 ctcgcctccg gtcgctttca gccaggcgag cgcgggcgcc atgtcggacg ccggaatagc
    31381 gtcctcgttg cgcgcgtaat tcatcgcgaa cagtccgagg aagccgtaaa tcccgagacc
    31441 ggaattgccc agcgggatgg cgaccgggaa ttccacttcg agcgtgatga ttacgccggt
    31501 ggcaggaccg ccctgcgccg gcggaatgtt cgccacgccc acgcctgcgg cgatgcgcac
    31561 ctgaacggga acgatggtga gatcgatctg tcccttgatc tcgttctcgt tgagctccat
    31621 gtatccgcgg ccgcgcagcg cgccggggat atccactgaa gccgcgaacg ccgtcagctc
    31681 cggaggcagt acgggatcga agcgcaggcg tacgcgtgcg cgctcgatgg acacgacacc
    31741 gagatcgatg gcgaagccga ggtcgacttc gaaaatgagc gggttgttgc gcgcgatacg
    31801 ggcgccgagg atttgtagaa tctgaccgag aggttcgacc acgcgtatcg cgccgggttt
    31861 ggagacgtcg atggtgtagc ccttggaggc gtcaaagatg gggcggaact ggaaggcggg
    31921 ctggccgggc tcgtagccca cgcggatgcc gacagcttta tagcgaacgg cgagcggagc
    31981 ttcgcgcgag atttcgatga gcgtcttgcc gccgatgctg acattcatcg aaatggcgga
    32041 ttccaggtcg aacaggacac tgacttcggg gccagtgggc cgcacgctca ccgcgatttc
    32101 accgccgtac cagatgacgc gctccacctt gacccatccg agggccgcga tagcggcagg
    32161 caccgtgaga atggcgccgg tgacggcgag ctcggcgaga gcgccatcgt ttgccacggc
    32221 accggcgccc gccgatagca gcggcatgaa gacaatcgag gttccaaaga aattcagtgc
    32281 gaagccggga tcggccgcga caggttcgtt gggcagcgtg ccccacatgt acaagccgtc
    32341 gatatcggcg ggatccgcgc cgtagtaacc gatgaccgat accgtgtccg tcgcgtcgtc
    32401 gatctgcacc acaacgcgaa cgtcgatcaa gccatcgcct gtgttgttcg cgcccagagg
    32461 ccgtcctgcc gctagctgcg gattcgcacc gatgttgccg gccagccggt tctccgcggc
    32521 cgtttggata tcgaacttgc cgaagacctc gcaggcgacg aacgtgttgc gcacgatacg
    32581 caccttgcag cccagcattc ggaaccaact gggcggcgag ggtggtgtgg cgggcggagg
    32641 atcttccact ttgatcgtgg gtggtggccc ggaaggctgc tccaccgggg ctcgcttgac
    32701 caacccttcg gtaatcgttc ccgccgtcga cgaaggcggc caggacgcca ctgccttcca
    32761 ccagatgcgc cgcgtggaca catccgggaa gccctggttg gtccagctcg tcatgtcggg
    32821 ggcgtgggaa agggtgaaat gcttggagct gagcgagggc tcggtctgca actgttcgac
    32881 gatggcgcgc agtccgagag cgcgccgccg ggctagatcc gtgttgtaag tgaccttttg
    32941 ctgggagctg tcgccttcgt aacttgcgaa gcccttgata gtgatcgcag tcccgtcggg
    33001 aaggctctgc aagactggac gcaaagcaga ggcgacatcg gtgccgccca tccatggcga
    33061 agtggtgccc tcgtcgggag agggttccgt gtgagtgttc tccggatcga gcgcatagct
    33121 gcgcgtggcg gtgtcggtac cggggtccgg tcggtcgaag cgatagtatc cggtgaaatt
    33181 cgccacgccg gctacgcccg gcagctccgc gcggacggtg acttgcgtgc ccggggccac
    33241 atcgacggta agtgtggcgc tggtgcccgc cggattgccg ttaaccgtcc actgcgtgaa
    33301 cgcggcgcgc gacggatcca cgtcgagtgc gatggtggcg gtggcgggag tttcggtcac
    33361 cagacgcaac cgcggcgcga gcacgggcgt tccgccctgg gtgatgctgg tggtttgcag
    33421 ttccaccgcc ggtgcggcag gttgtgtttg agagcccgca ggcggagccg cgatggacgg
    33481 ccgccggctc acctgaatgg cgagatcggc ggatttcgcc ggcgcgcttg tgtccgtggc
    33541 gtggatctcg atcagcctgg tggtttgcgt gccgaggtcg acatcgaact cgcgtcctgc
    33601 ctgggccacg ccgccatcga aggccgcagt gaccgtaaag ggagtgcggc ctccctctac
    33661 gtcgatgacc atgcgcgtcc gcacgggcaa ttgcgctgta gcggttgtat cgctggcgcg
    33721 cgtgatgcca tagccgcgcc catcggcgtc atagaagcgg gcgcccagct tgagcggtcc
    33781 cgcgccctga ttgagcacgg tcagctcgaa atcgccggtg acgcctgaag aaccgccgat
    33841 cccaatcagc aggttgcgca cgccggcatc gaccgcgaag tctttcgccc cgtgaggtgc
    33901 gacgaacaga cggatttccg gaagatagag gccggtccag ctttcatcga agccgaactg
    33961 cgaaagaaca tcgggcggcg tggaaccgtt cgaaaggtcc agcacgccag aacgaaaacc
    34021 gaaccccacg acgttcgaag gcccgatgaa tgcgtaaggc ggatccatct tgatcagttc
    34081 cgccacggcg atgtcgccgg gatcatccag tccggtagca cccgcggaca gcagcgtggc
    34141 agcgatcggg tcgttattcc ccgagccctg tgtgagccgc atcttgatgc ggggaagagt
    34201 gatcttgacc tgttcgtggg cgggatcggg aaccaactga ccatctggct ggcgcgtggc
    34261 tccgcgcagg aaggggggcc ggagcacaat cgtggtgagc aggaagtcca agacgaactc
    34321 ggtcgtagcg taatcagatg gcggcgcgtc gagcggattc gtatcgtagg cggtcagcac
    34381 agcggcggtg gtggcgaacc cggggtcccc accgatcgcc gttaccgcgg tggacacctt
    34441 ctgggaagcg gtgcgcggca cgacgagctg aaactcaagt ttggtgtccc gaacatcgat
    34501 ccacggatcc cgccgtgcag ggtcgttctg tgggtgaccc tcggtgttct ccgcctggat
    34561 gcccataatc atccgggtgg gatcgatgaa gggccgaacg gcaccctcga agctgacaac
    34621 accgcgaatg acgatgccgg cctcgtcaac ggcgatctcg tgctcgcgga cgagaagtac
    34681 agagagagcc gcgtgccagt ttccaaaact gtcgccgcgc aagatgtacg gtgtaatcag
    34741 atccagcaac gataggtcga acggcacggc acccagatcc ccctaagtcg ttggcactcg
    34801 atgggacttt tggcttgcct tctgctgagg caataggcga attataaagc cgcgtattgt
    34861 gcgtgtcaag caaaatgtgc ttcgattcta ttgtgattta tcacttttcg gatattacat
    34921 tcagtttctt tatatatgtg cccaacttac tatacgtaaa tcgagtacta tattacacaa
    34981 gctgagtact atggtctgag ctgctctttt tcgtgcgttg acttaacgca ggtgagcgga
    35041 gcgccacccg gcctgcgggg gcccaaccgg acagaagcaa gagcgtccaa tgtgttatgt
    35101 tgtacgccat tgtggcggca caacaagagc acgatcgacc ctctaggacg atctcctcat
    35161 gctgaacagg cgaactttcc tcggcggcct gcccttgctg gcggcaaggc cggagccgaa
    35221 ggccacggac gtatggtctc ccccgcgttg cgaggtctgg gacgtacact gccacatttt
    35281 cgaggtggcc ggagagacgc ccgaagcgcg ggtgaccgag ttggtgaagt tcggggaccg
    35341 gatgggcatt gagcgatttg tgctcttcat gggcttcccc ttctacaccg atcccacgcc
    35401 ggagcaactg cggcagcaga acgaccaggt gctacgcgcc atgcgggcgt ttcctcagcg
    35461 tgtctcgagt tttgtgtacc tgaaccccaa ctacctttct ttcagcctcc aggagttcga
    35521 tcgttgcgtg cgggacggcc cgatggtcgg cgtcaagctg tgggtggcga aacactgtaa
    35581 tgctccggaa ctcgatccga tcgtcgaacg cgcggcctct cttaaagcgg cgatcttaca
    35641 gcatacgtgg ctcaagagca ccggtaatct gccgggggaa tctacgccgt tcgacgtggc
    35701 cgaactcgcg ctgcgccacc cgaacgttcc gctgatctgc agccacgtgg gaggagactg
    35761 ggaaagggga atccgctcta ttcgcgggac caagaacgtg tacatcgatg tcgccggatt
    35821 cgatccgacc gctggggccg tcgaaatggc agtacgggaa ctgggcccgg agcgaattct
    35881 gttcggcagc gacgttggca tacggacgtt tgcgtcgcaa ctgggcaagg tgatgggggc
    35941 ggaggtttcc gacagcgcta gaaaactgat cctctgcggc aacctgaagc gcatgcttac
    36001 tccgattctt aaagcgaaag ggcgccggct gtgatcatcg atacaaacgt gagcctttcg
    36061 cgatggcctt tccggagagt gccgggtgat gagcctgccg acctggtgat gcgcctgcgc
    36121 gggcgcggtg tcgggctggc ctgcgccggc agcttcgacg ggattctaca caaggacctt
    36181 gccggggtga acaccaggct cgcccgcgaa tgccggacgt acgggaagga gctgctgctc
    36241 cccttcggtt ccgtgaatcc gaagttgccg gactggcggg aggacttgcg gcgttgccgc
    36301 gaagaacacc ggatgcccgg aatccggctg tatccgaact atcatgggta cgacctcaag
    36361 gatccggctt tcggcgagtt acttactctg gcgagtgcgg gcgggctgat cgtacagctt
    36421 gcaatgcgca tggaagacga gcgcacacaa catccgctac tgcgagtgcc gcccgtcgat
    36481 gccggcccac tgcccgacct gattcgaggt cagcctggag tgcgaatcgt gctccttcat
    36541 tggaagggac tggtaggccc agcgctgctc gagcggctgg caaccgccgg cgaggtctac
    36601 ttcgagatct caatgctcga aggactcggg gccctggcgc ggctcataca gcaagtatcc
    36661 gccgaacggg tcctgttcgg atcgaacttt cctctgtttt acttcgaatc cgcactcttc
    36721 aaagttcgcg aggccggcct cacgcaactg caacagaacg cgatcttcga ggggaacgcc
    36781 cagcgcttgt tgcatctgcg gtgacctctg ggcacacacc cgaggcggca cgggtacgcc
    36841 agtgccggag ccaggcgaaa gaactgggct accgcattga gtgcggcgtt tccagtttca
    36901 acgcgtggtt ggtgggagcc tccgggcaat cgacggtgta actgcctcct ccggcgggca
    36961 cttccacgct tttcaaaatg cgctcgtcgc caatggagta gtcgtgcgtt acccgcacca
    37021 cgcccgcatt caccgggcgc ggatctcgac agtgcgcgta aatacggata gcgttcaacg
    37081 aagggtcgcc ggtgtaacgg acccagactt tgcgcacggg ctcgggcagg atcacatcgg
    37141 tgtccatgct gtaatgccag tgctggttcc agtccggtac cgtaccggtc ccgccgtaaa
    37201 tggtgcggaa aggtccctcc agcgcggtgg cgatctcgat cttattggcg gtgagtggcg
    37261 cccgcttcag gtgatgcgcg cggaacatgc cgcccacgct cagccattcg atcacctgcc
    37321 cggggcccgc ttccactggc gccacggcct gaccgcgcag gcgttggctg ggctgcctcg
    37381 gctggtaatc gcccacgatc cagatgccgt gcctccgaat gtcttccgga tcgcccaggt
    37441 gaggcagcat gggcagcagg cgcgtgggcc ggccacgcgg gtcgttgaca cccaggcgca
    37501 ggacgttgcg gcccttggcc aggcgcggaa gcgagatcgg cgccacctgc ccccaggttc
    37561 gcaactcgag cgagcgcagg actgctgcgc cgggctctcc ttgcatcccc agcctgacga
    37621 agtagtggta ccggccgcac acaaaccgcg tcaggtcgta gcgcaatttg ttgggaagcg
    37681 cgccgagaga tacccaagtg tggccaccgt cggtactgat atcgccttcc acggcggtcg
    37741 tggcgtcgat cattatagtg gccgcatcac gattcagggc gcccgccgga tcgtcaaggt
    37801 cgtccatcac aggcacgatc acgtaagggc tgagtacttc aaagacggcg aaaccactgc
    37861 ctcgttccgc gggcgtgaga ccagccgcgg atgtcctcag gttacgcact tcatacgcac
    37921 ccagttccac gtcgcgcgtc ttttcggtga ggtccggtcg gtacagcagc agcgacgagc
    37981 ccacgcccca gatgctgaaa ttggcgttat tcggcttgat gccgcagggt ggtttttcaa
    38041 gcagctggcg catccagccg gtataggtgg gggaatggtg ccatcggccg tcccggtttt
    38101 gccaccagcg cgtgagggat tcgccgggcc gaagcacgta gtccatgatc cagccaccca
    38161 cgctccagtg atattgcagg tcaatcggtt tggctgaaaa catccggaaa cccacacctt
    38221 tgtctttgac gttggggaag aacggctcta ccgtgtgccg agggtcactc cacaggctcg
    38281 ggtcggcgcg ggcctcggcg gcggaggcta ccacgccgcc gggccgcagc agcgtgccgc
    38341 gcagatccac gtcgaagtag tgccagcctc ctccataaaa gatttcggta gccacgtggt
    38401 tccacgccgg cacgctgaag ctgcgggcgt caaacccggc cgcacggaac acgccttcca
    38461 gcaccggccc gtgaatcccg cagaagccgt gattgtggac gttcatattc ctgagtgcgt
    38521 cacggctgtt gccgagggcg taatctgtat ccgcaccgtc gaagatctca ttgaagtgat
    38581 agagaccggt gctgaggccg ctgaggcgct gccacagcgc gatggcaaga tcctgtccgg
    38641 acagccccgg tctttataga acgccacgaa gtggccgagg ttggtgtaat cgggttcgcc
    38701 gtccacgttg atgcgtggtg aaaccaccac gccctgtggc gccggggcgc acacggcgac
    38761 catcgcagcg atgaccagtc cggatagaac acgcgtcagg aagatcgaga ctcgtcccga
    38821 catccgcccc caatctcaat tgaccggatc gaaccgtacc ctcagcatgc cgtagaatgc
    38881 cgcaagaccc gccagctccg ccttcgccct ctcgctcggc tccattacct tcaccggcct
    38941 cggcgctacc agcgccacca tctgccgaat gtcgaactcc tccagcaggc ctaagcagaa
    39001 caactccgga gcgtccctga ctgtcatgtt cttctcgata atctccttca ggctgccgaa
    39061 cgattcgtgc aattgcaggc tcgcgatcgc ctttggctcg attcccgccg ccaccagcgc
    39121 aaacaggctt gaccgcgggc cgatggccac tacggtaacc ggtccggcgt tgcggctctg
    39181 tagccaccgc gccgccgcca ccacctggct cgcctgtaga cccagtggcc ggtcgcctaa
    39241 agccgccaac aggatcgcga acagccagtc ccttttgtcg atccgcgatt cgccgaaata
    39301 gaacgggtcc atcgccacca cccgctggcc tccggccaat agccgctcga tctctgccgc
    39361 cgatcccgcc cgaccgccat cggctacgac caacgccgtg gactcgtgtg tacccgaggc
    39421 cagttccacc gccggcaccg tccaagcctc tcccatctga agcttccaat aggtggcctt
    39481 gagtccgtcg gcaccctgcg gcgtgtcgct tcccgcttca atcgcctcca ccgggaagtc
    39541 atacgcccgc acgatcccgc gcagcttcga ccgccctgtt tccttccacg ctgccgctgc
    39601 tcgctgatcc tccggcaatt ccggccgccg aggcaactgc cgactcaggt tcatcgcaat
    39661 ttggtggaag ctcagattgt ccgccggcag cgggctgttc agttgctgtc gtgtccggat
    39721 ctccttttcg accgggatgt cctggatcga aaagctcggg tcttccgcga agaagtagtc
    39781 ccgcagcgcc cggtagaatg cttcgcggtt atcctggccg taattgtgcc cgtcgtcgta
    39841 gttgatatgg tagcgaaggt tgcccggctt cccgaacagc tcgtagatcg gccgcgccgc
    39901 catcagcagg ggagacaccg catagtcagc gcggaagcag caattgtcac gggaattgtg
    39961 cgtcagcagc gccggccggg gcgccagcat tcccgtcaga tgtgtgtaat ccgccactga
    40021 gcccagatcc gtcggtgtct gctcggaatc cccaaggtct ggctcgggcc actgcgatcg
    40081 cgtcaggtag ctcgagtagc ccgccaccgg gttcgccagt ttcactcgcg tgtccagcga
    40141 actgatgata atcgtctgcc atgagccccc cgagaggccc gtcaccgcca cccggttcgc
    40201 atccgtattc ttgtgcgcca gcaaaagatc gagggcgcgc ttctgcgcca gaaagtggat
    40261 tcccactccg cttgtacccg tgaggtccac ctggttcatg cgggcgtggt cgaaatcagg
    40321 aatgtcgagc tgtcccttcc ccacccattc cgggttgagc gccagtatcc cgcgtttcgc
    40381 caggttgatg caccgcgcct gtatgtacgg agtcgccttc ccgatttcct catgcccgtt
    40441 cacgttcagc accgccggga cctttccgct cagcattgct ggctcataga gaagtgcggg
    40501 aatccacagc cccggaagcg cttcatagcg aagcttcttg acccggtacc cttcggcatc
    40561 gatggcgtca agccactcca cattcgtttt cgcctcgttc caccgcgccg cctcgccgcg
    40621 caggatcgcc ttcgccagca cctcctcgcg aagtttcgcc gcgtaactct cccattcggc
    40681 ggcggtgcgg aagtgtggta ccgggaggac cttagccccg gtgtaaacct ggacctccag
    40741 cgtgccctgc cggagcccga gaatcggctt cttgagagcc tccaccaccg cggactgctg
    40801 ggcgtacaaa gagcaaagca tgaatggaat gacgaggacg gatcgcatct tcagtctcct
    40861 cactacctgc aaaagcgttt cgaacgggac ttccattctg gacttcgtgt cctgttcagt
    40921 ataacggtgc cgccccaacg ttacagtgat tgcgtcttgt gtgtgcatcc gactagatcc
    40981 gaggtcgctg agggcgaatg acgctgacga tatcgggatc tgcggcttct gactctttcg
    41041 cctatcaggc gcgcttgcgt actatcattt agcagagctt gctcgaacga gtggtcttgg
    41101 aaggaggctg gtagcttgaa gcgacgcgaa tttctcgggc tcacgacgat gtcgttgtct
    41161 tcgcgcgcac gaggtcaacg gcaggagcgg cctcctcagc caaatttcgt tttcattatg
    41221 gccgacgatc tcggcgttta tgatctcggc tgctacggcc agaagttgat ccgcaccccg
    41281 aacatagacc ggcttgccgc cgaaggcacg cgattcacgg attgttatgc cggcgccacc
    41341 gtctgtgcgc cgtcccggag cgtgcttttg accgggcagc acacaggcca cacaaccgtt
    41401 cggtcaaatg catctcttcg tactggacgt cgcgtttcgc tcacgacaga cgatgtcacc
    41461 gttgctgaaa tcctgaaagg tcccgaatgg cgagacgcct tcgatgacgc gcgcggctat
    41521 gcaacggcca tcttcggcaa atggggcctt ggcgagccgg gtacgcccgg tctgccgaac
    41581 gatcacggct ttgacgaatg gttcggcttc ctaaatcaac aacatgcgca cggtcacttt
    41641 ccggactacc tttggcgcaa taaaaccaag gagacactga agggaaatct cggaggtggc
    41701 cgtcgcgagt acgcatcgga cctgttcgcc agggaggcgc tgtactttat cgagcggcac
    41761 atgtacgagc cgttctttct gtacttcacc ccgaccgtgc cgcacgccag gttcgaagtg
    41821 ccgactttgg gtccgtatgc caacgaatcg tggcccgaag aggcccagaa ttatgcggcc
    41881 atggtcacgc ggatggatac ttacgttggc cgaatcatgg ccaagctgag ggaaaccgga
    41941 ttggacgaga acacgatcgt cttcttcacc agtgacaacg gcggcatttt tgagttcaag
    42001 ccctttggaa caaatggacc actgcgcgaa agaaagggtt ccatctatga aggtggaatt
    42061 cgcgtgccga tgatcgtccg ctggcctggg agaatcaagg tgggagcagt gagcagcttt
    42121 ccttggggat tctgtgacgt tttgccaacc ctggccgacc tcgccggcgc ccggggcctg
    42181 cctaagaaca ttgacggcat ttcggtcgcg cctgctttga tgggtaaggc gcagagcgca
    42241 gagcggccgc tgtactggga gtcgtacgag gacggtggat ttcaccaggc cgttcggatg
    42301 ggtcgatgga agggcgttcg ccatgggctc acacgtccac ttgaagtgta cgatctacag
    42361 accgacatct cggagacgaa agacgtagcg atcgcccaca agaacatcgt cacgaggatg
    42421 gaggagtact tacgcaattg ccggaccgac tcgactgagt atccgatcga ccggaaagcg
    42481 aattagtata ggtatccgtc gccaggtgcg acccatccat gaagcgctgg cctgtcggtt
    42541 gcctgcgcga agctggctgg taccctgtga cgaccgttgg cttcgtttcg ttcttcgcct
    42601 cgcgtcatcc tgcttgagtt tcagggattt ccatgatgta ctcgtcgcta tgaaattccg
    42661 cttttggcac gtcatgccgc tggctgtatc gctgagcgca caggtgaaca tcactcgaca
    42721 agccggcgag attgcggtag agatcgacgg taaaccctac accgtttttt atctcgcccc
    42781 tgggggcaac aagccgtacg tgtacccctt gcggacagcg gacggccttg tcgtcacaag
    42841 gcactttccg atggaggagt ttcccgggga ggtcaaggac catccacatc atcgggggat
    42901 gttcttctcg cacggggata tcaatggctt caacttctgg gcaaccgagc ccaactcgaa
    42961 gagcccaaag aaggccagca tggccctgaa gaaagtactc gaggccaagg ggggtaagaa
    43021 atccggcacg attagggcaa tgttcgacgg actcgatccg atgggcaagc cgatcatgac
    43081 ggagacgcgg acactcgtgt ttcactccgg gccgaagctg cggatcatcg attacgagat
    43141 ccagatcgat ccgcttgaaa cgttgaaatt cggagacaca aaggaaggga cctttggcat
    43201 ccgtttggcg acttccctgc aggaggacaa aggcggcaga atggtcaacg ccgagggacg
    43261 ggagaccgag aagaacgttt ggggcaagcg atcggcatgg gtggattatt gtgggccggt
    43321 tgacggtcaa actgtaggcg tagcgatctt tgatcacccc tccaatccgc gacatccgac
    43381 gtactggcat tcgcgagcct atgggctcca cgctgccaac atcttcggcg tgcatgattt
    43441 cgagaacgac aagaccaaag acggaagcat gacgctgaac aagggtcaga ctctgcgctt
    43501 ccgttaccgc attgtgattc acccaggcga cactaaatcc gctgacatcg ccggcctgta
    43561 ccgggagtat tcaacaggga agtagcctgc gtgacaaggg gaaggagatt ctcatgcgaa
    43621 gacatccttt cgggcttgga gcgatcggtg ttttacttct tgcaggcgtc gcgtcgacgt
    43681 tgggccagta ccagcagaag aacgcgaaag aatggaacaa ggatccggag tggcaggagg
    43741 tacgatacgg agcctggggc ggccctggcg tgaatccagc gcctggcgcg atggattcga
    43801 tgctgctgaa ggactacgcg ccgaaatctt cagttgtggc ccagcggacg tccgttccca
    43861 aggccaagta ccccgcgatc gacgtccaca cgcatgtgct ggcacgcaca ccggaagagg
    43921 tagcggattg ggttaaaacc atggacgagg tacgcattga gacatcggtg gtgctcacca
    43981 gcgccactgg cgagaatttc gataagctgg tcgacctgta cctcaagaag tatccgaccc
    44041 ggttccagct ttactgtggc ctcctgacca ccgacatgga caacccggac tatccagcac
    44101 gcgctgctgc ggaactggaa cggtgttacc ggaagggcgc gcgcggagtc ggtgaattgt
    44161 ccgataaggg ctggggcttc agccgaaaca cgaatttgcc ccgtgacaaa cgcctccacc
    44221 ccgatgaccc gcgcctggaa gctttttgga ataagtgcgc cgagctgaag cttccgatca
    44281 atatccacgt gagcgatcac ccgtcgtgct ggcagccgct cgacgtctac caggagcgca
    44341 cgccggatta ccagcacttc aaccagtacg gtaaggacgt accgtcgtac gaagaactgc
    44401 ttgcgatccg gaaccggact ctggcgaggc acccgaagac gatcttcatc gcctgtcatc
    44461 tcggcaatca gggtaacgat ctggcagcgc ttggcaaggt tctcgactcc tatccgaatc
    44521 tctacctgga catctcggct cgtgattacg aagtcggccg gactccacgt gcggcggcaa
    44581 ggttcttggg gaagtaccgg aatcggatcc tgttcggcac tgatatggga cgcgagaaga
    44641 ggatgttccg ggcatggtgg cagttgttcg aaacggccga tgagtacatt cctggccgtg
    44701 tctcgtggag gtattacggt ctcgagcttc ccgcgcccgt gctcgaaagc ctataccggg
    44761 gcacggcgag gaaggttatg aactgggaga aactctgagg agattcttct cagaaggtaa
    44821 ggagcatatt ccatggatcg aagacatttc ctgatgagtt ccgtggccgc gtcggcggcg
    44881 atgcggccca gtatgctggc gagcccgaat gacacggtcc gggtagcgtg cgtcggtttt
    44941 cacggccagg gacaggctca catctccggc tacctcaaat tgccaaacgt cgaattggtc
    45001 gcgttgtgcg atgtggacga agcggtcctt aaggacggga tgaagatggt ggagaagacc
    45061 ggaaagaaac gcccggcggg ctacaccgat ttccgaaagt tgctggaaga caagtcgatc
    45121 gacgctgtgt cgctggccac gccgaaccac cagcacacac tgcaaaccat ctgggcgtgt
    45181 caggccggca aggatgtcta cgtcgagaag ccatgctcgc acaatatgtt cgaagcgagg
    45241 cagatcgtcg cggcatcgcg gaaatacaac cggatcgtgc agcatggcac gggtgcctcc
    45301 gcgtcactgc acgaggccgc gcagcaagtg cgcgaaggga ttatcggcga cgtctacatg
    45361 gcgcgagggc tctgcttcaa ggtccgggac acgatcggcc gtacgccgga ggaaccggtt
    45421 ccgccgggag ttcattacga cctgtggctc ggtccggcac cgaaacgtcc cttcaccaag
    45481 aaccggttcc attacaactt ccattggttc tgggattacg gtaacgggga cttcggcaat
    45541 cagggaagcc accagatgca tgcggcgcgc tttgtcctcg gggtgaaata cccgacgaag
    45601 atctccgcga ttggcggcca cttcctgttc gacgacgatc aggaaacacc caacacgatg
    45661 gtagccagct acgagttcaa cgagggcggt aagaagaaga tgctggtgtt cgaggtccgc
    45721 cattggctga cgaataacga ggcgggcatc ggcgatcgct ccgacgcgtt cgcaggctcg
    45781 gcggtgggag cggcacagac tgtggggaac ctctattacg gctcgaaagg ctacatggtt
    45841 tatgccggag gctttcgcac attcctgggc aaagaacagg cgcccgggcc aaagtcctcg
    45901 ctgggaagga gggagagcgc cttcgggaat tttgtggacg cgattcggag ccgtaagcgg
    45961 gaggaccaga gagcggaaat cgaggagggt gccatctcga cgatgctcgt tcacttgggt
    46021 aacatttcct accttcttgg ccggacgctc cattttgatt cgaacacact gacctgtatc
    46081 ggtgatgccg aggccaatgc gatgttcacg cgcaagtacc ggccgccatt cgttgtaccg
    46141 aagctcgtct agccgtggca attcgaagcg ggcgatgagc agggagaacc tctggccatg
    46201 ttccggcatc gcatgacagt caccgcagtt ctcatggcgc tcgggtgttg cggagtcgag
    46261 gccaccctgc gggcgcagag acctgtaccc gtggagatca ggatacaggc tgaccgcacg
    46321 gttcgaagaa tgcaaggcgg cataggggcg tcgtttcacg ccatcgagga accgaagccg
    46381 ggccagcggc ccaccggcgg cgtctgggcc ggcagtgcct ggggagcgaa cccgccggcc
    46441 gaagatgagg cggcgtggaa gtcgctgttt cattacgcaa actggcttgg cctggattgg
    46501 tgtcgggtgg aactcgagca gcgaatgtat gagcctcggc ggcgccggtt cgagtgggac
    46561 aacgcggaga tgcggattct ttaccgaatc ctggactggg cggaaaaaca gggtgttgat
    46621 gtgttcctcc agcagatgtg gtccaacgtc gaatggaatg cgtaccctga gatgcgcgca
    46681 acgatggagg gacggctgcg gagcgcccct ttatcgatcg acgatttcgc ctatggctta
    46741 ggcgaactgc ttcaacattt gacaaaggtg aagggttacc gctgtattcg ctggctttct
    46801 atcaataacg agcctggctg ggattggtca tggtggcagg ggccggacgg tcagcctgtg
    46861 ccgatcacac cggccttcgc agcggtgcga aaggagctgg accggcgggg gatcacggtc
    46921 cctctctccg gtcctgattg gacggacctg ccggatctgg agccggagag gattgatttc
    46981 gactcctaca tcggggccta cgacctgcac tcctacaatg cgtatttcga tggaatccga
    47041 ttcggctacc cgttgtccgt ggctgagaaa cggctcgcgc gatgggcaca gtgggcgcac
    47101 ggccggggta agccgatgtt cctttccgaa ttgggcacga tggcctttgg ttttgtaggg
    47161 aaagagccgg ggccgggtgt gtatgaatcc gggttaaaag acgccgcgct cgtggtgcgt
    47221 ggcatccgcg ccggcgtgga cggattcaac cggtggagtt tcataaaccg tggcgacctg
    47281 gacgggcaat ggcagatggt ggacacctgg gacatcgacg aaggcaagct caaggatcac
    47341 tttacgccgc atccaaacac gtactatttg ttcgggttgc taagccgctt caccgcgaag
    47401 cgctccgacg tgcttccggc caacgtcgaa ggcaagttcg acccgggagc aaggcaactt
    47461 gcggcggctg tgttgcgcag cccgaggggg cacatcagcg tgctggtggt gaacgagatg
    47521 tactgggacg cggacgcgaa gatcaccatc accggccttt cggggaggac gctgctctac
    47581 cgttactcga tcacgggcgc cgaccgggat cggacggatg tcgtcgtgaa accagccgct
    47641 aagttcgact tagacgccag caggagcagt gtgactgacc gaatcccgcg gcttagcgtc
    47701 accgtgtact ccacgtacga gttggaccat gggcagccgg gagtaatggc ggaaaacgac
    47761 tcgaagtagt ccgcagccgg aatcatctac tgcttgtacc accctacggc gtcaccaccg
    47821 tctccatctt gccgccttcg ctcactcgta ccgccagccg gcctttggta tcaatgccac
    47881 ctccggcgag cgtgccttgc gcgccggcgc cgatccacgc cgccaccgcc gagtccgcga
    47941 ccttgaacac tgccgtgtcg ccggccttct ccaacgcgat ctccggctct gcttctgacg
    48001 cccggtacgg caccatcacc gcgacaaact tgatctcacg caccggcttc cccgtgcgcg
    48061 cattcagatg ccactggttc gcgaatcttt ccctgcgcgc cgcgagcgat gcggtgcgcg
    48121 ccgtggcttc atccagttcc ggctgtatgg ggaaccaatc ccgctgcgag aaccggaacg
    48181 gctccgtcgc gatcaggcga accaccagcc tcgcatcttc gttctgcacg cgaataacac
    48241 cggctttgtc gtcgagcttc atctggttca gcgcgtgcaa cagccagtcg aaagtcgccg
    48301 gcgcgtctgt tcgcaaatga tcatgcacga cgaaaaccgg tcgtgtcttc gaggcgataa
    48361 aggccactgt gcgctcaaag ctctccacct tgggctccat gggtggaatg ggttccttca
    48421 accactcact ccactgcttc ttgacgctct ccctctgcgg aatgttgtac gccttcgccg
    48481 cctggccgcg cgccatcgtc accacaccct ctcggatgta atcctcgatc gttccgttcg
    48541 ccgcccactc gaagggcggc tggccgcggc cgttcaccag gactgcgttg tgcgccctcg
    48601 tctgccgcgt ccagagctga tcgtgcgggc tgccgtacca ggggtagtag ccggaatcga
    48661 tgagaagcgc gcggccgtac gcattcaact ggaagctgtt ctgatccgcg tggctgtggc
    48721 tgaacgatcc gaagcggctg gacttgaaca gcgaccagac gtcctccctc gcgtcgccga
    48781 gcgcggagtg catggccacc caaccgatat cggtgaagaa cctcgatccg tccaacttcg
    48841 atggtggctc caccttcggc tctccccctc ctgctggccc gatccgtagc actgaaccca
    48901 cgttttcgat tccgaactgg gccggagtgc tgcgctgcga gccacccctt tgttgccggg
    48961 acgggatcga ctgggcggtc caccgcagca ccgggtcgtc gaaggcctcg gcaagccttt
    49021 ccaggattcc acgctggctc ccgcccggcc ctcggtccga gccatcaccg aaggcgccgc
    49081 gttcagcgta cgggggaatg caatagaccg ccaggtatcc ggtattgcgg tagaagggcc
    49141 ggcggaacag gtcgacgtcg gtgacaccac gcagcgcctc cgcgaagccc gtgtgatagt
    49201 tcacataacc gccccagtag ctcagcccct gcgcccatcc accctcatcc ccgccccagc
    49261 ccggataggc ggtcaggtag cagcgcagga cgtagtcgag ccacttctcg gcgtccggaa
    49321 tgtcgcccag gaacgacacg cccgcattac cgagaaacgc gagcacgcgt ccttcgtgat
    49381 tcgagaacgg atgtgtcaaa aagtcgattt tcttcagaag ctccagcacc tggttgccgc
    49441 gctccgtcat tgcagctagc accttggcct tctcgctggg ggtcaattcg ctacccgccc
    49501 aatcccacgc gagggacatg cgatccagaa tgggcatgga cgcctcgtcg ttgccctccg
    49561 atccgttggg ctgtagcacc ttgtggctcg tgacgccccg cggatcccag cttgcgagtg
    49621 ccagcatcca gcggcgcgca ccctccaggt acttcttatc gcccgtgatc ctgtaagcca
    49681 gcgcgacccg ggccaggtgc gagctgccgg ccttggccgg agtgtaaatc ctccgccact
    49741 catcgacgtt gaactcggcc gatgggtatc ccgccggctc cgcgtaaggc tgttcggcca
    49801 gcgaagcatc ggcttcctcc agaaacgcct tccaacgcgg aaggttgccc gaggccgccg
    49861 ccgtcttgac ttccgccagc cgttttcccg acaggaacag ccgcggccgc accttggcaa
    49921 gttgttcctt aagccgcgcc acgtcgggca tcaccagcgg cgcgagcccc ttgggaaccg
    49981 tgaaccggcg ggccttcgag gccccggtca ccgcggcgcc atcgaggtaa accacctgcc
    50041 agacataatc gcccggctcg aacgcatgcg acattgcgta cacggtcgaa ctctgcggtt
    50101 cggtcgcgag aatgcccgac gaactctcag aggagcggcg cacttcgagc cggtacgtct
    50161 tggctttctc gttcggagtc cacgtgaacc cgggaggatt cacgcgaagg ggctgcccgt
    50221 ccggcgggta cgctgccagt tcttcgactc caggctcccg ctcgatcacc gccgccgacc
    50281 tcgtccaggc gtacttcttc ccgccacagc caatcgtagt gagcacaagc agtacgagtg
    50341 tcgagacgaa atactcctca tgacggcgca tacgaatctc cttcaagtgt gttcacgaat
    50401 ccctgtcagc gggaatgata tcaccgaaag ccggcagaac agctattgtc cggtctggcc
    50461 cgggcgctcg tccagatcgc ggatccagat gtctttgaag cgaatccgcg acggcttgcc
    50521 aggatgaatt tgcagtgcga caacaccatt cgtgtttcgc gggtccgggt aggtgaagtc
    50581 aatcatccgg actccattta agtacgttcg gacacgatgc ccctcgacct ggaccagcat
    50641 gtcgttccag tcgaacggcc tgaggaccgt ttcgagttcc ggcgccggcc agacgatcca
    50701 gccacggccc ttggtttcag gcgtgtgatc cccatgtagt ccgcctgtgt gaccacccac
    50761 agaactcgag atctccactt ggatgaactt gatcttgctg atatcctcct caatgtcgga
    50821 gtgatagaag aacccgctgt tgtttggtgt ctcgcatttg aatcggagaa acgcatggaa
    50881 attccgatac tgcttcagtg tcttcagaaa tccatccttt ccgtggatac cctctccaag
    50941 gatcgttccg tcgtccacac tccagcgttc aggaccgacg accttccacc cggccaggtc
    51001 ctggccgttg aacaagggct cccatcgcgc gtcgaagccg gcaacgggca attgcttctg
    51061 ctgcgcagcc atcgacatgc acccgagcac gagaagtgca ctccaaacgg aaagcctgga
    51121 catcgttata cctttctttc aaatccacag acggtgcggc tgtctgcgag cggcagccag
    51181 agaatgtctc ggatggcggc gtagaatgcc cttacaaatc tacgccgaga tcggcccagt
    51241 taacgacctt ctctctaacc atcgcctcgt ggccgaggat tgatgtcaac gcagccgtgg
    51301 ctccaacggt gatatccgct ggagaagcgg cgcccttcgt aacgcagtcg taaaaggtcg
    51361 tgatgtgcgc gtgcggcgga tcttgctgct tttccgtcaa cgcggacggc tggccctctc
    51421 tcgccagcgg gtacatcatc gccgtgtaca agaggtccac cgcgcccttg gtcccgtata
    51481 cgtaaaccga ttgattgttg ttcggcatag tccgaggatg aaatacgttc tgggtgaacg
    51541 acattttcac gccattcggg tagtcgaaga cgagcaggcc acagtcgaaa atcgtacgtc
    51601 ccggaggatc gttcttgaac atttgcgttc ctccaaagcc gcaggcgcgt gacgggcggg
    51661 caccgatcac ccagttgcag gcatccaggt tgtgcaccga ctgctcgatc agatagcctc
    51721 cggacttcgt cacgtcgaag taccagtcgg cggaggaccc ggtgtggttc aggtcaccgg
    51781 acgcatgacg ctgcgctttc accatgatca catcgccgat cgctccttcg cgaactttgc
    51841 gcacggtttc ggcaagctgc cgctcggaac gcatctgctg ccctgcgacg aatacccgat
    51901 ttgtgctctt ggccgctttc acaagttcac ggacctgttc cgcggtaacg ccgatcggct
    51961 tctcgcagta cacgtgcttg ccggccttga gtgccgccac agccatttcc gaatgcaagt
    52021 acggcggggt agcgataaag acggcgtcga cgtccttccg gtcgatgatc cggcgccagt
    52081 cggcgtacgt ggccgggttg tccctggcgg cggttgtcgc cgccttatcg agccggtcgg
    52141 gcttggtgtc gcatagcccg acaactttgg cgttttcctg cgcaagcacg ccccgcagca
    52201 ggtaggagcc acgattgccg actccgatca ctgctgtggt gacgggcggc ccagattgtc
    52261 cgtgtacgac gcggaccgca gccgccgagc cgaggagaaa agatcgtcgg tcgattctca
    52321 tattcaatca cctcctgaat agtttacgtt aaagaagcag cgcccctttc caatctggtc
    52381 cgcgcggacc tgagagatag tcggcgagca agctgacgct tcaggcttgc cgaaggcggc
    52441 ggaagttggt ggccggaaac aaccactttt ggttgagccc ggccagccgc cgcagacaca
    52501 gttgttgatc tcacgggatt taggaactgg cacgcaagtt gccgtcccga caggcgtgat
    52561 acccaacaga caatcgtggc ttttcccggc ggcgctcgca atggttctgt gcggcccgct
    52621 gccggcgcaa gaaaccaatt cccccgaggg tggggccacg agcggatggc gcaggttcga
    52681 cgaggcggca cccgcgagtc cggcctctcc tgcagggcaa ctcaccttgc cggccggaac
    52741 ctggattacg gtccggatcg accaggagct ttccagcgat cacaacttgc cgggagacgg
    52801 cttcaccgcc tcactggttc agccactcgt ggcgaacgga ttcgtggttg cccggcgcgg
    52861 ccagacagtc ggtggccagg tagttgaggc gcaaaaggct ggccgctcaa aaggcacatc
    52921 ccggctgggc atagcgctta cggaactgag cctggtggat ggacgccagg ttccggtgac
    52981 aaccaggctc acccaatacc agggtggcag ctctgtaggc cgtgacgttg gcgctgtagc
    53041 gacgaccacc ggcgccggcg ccgccatcgg agccgctgcc gagggcggtt tcggtgcggg
    53101 catgggtgcc atcgctggag ccgcggcagg catgattggc gtcctgtcca cccgcgggaa
    53161 ggccaccgtg atctacccgg agacgccgct taccttccgg ttggaggagc cgctgaccat
    53221 ctccacggag tcctcggcgc gagcctttcg gccggtcgtc cccgaagatt atccacagga
    53281 gccaacgttg caccgggagc gccgggtcgc cgtagcacct ccgccgcctt attactacta
    53341 cggtggctgg gattggtggt acagccccta ctactatccg cgcttcttct acggcccgag
    53401 cttctactac ttctccggcc cgcgtatcca tggcggccac cgtggtttcc ggcgctggta
    53461 gcagtaagca tgaaatcggc tgaggcgccg accaccgccg cgccgtgcgc agcagcgaga
    53521 cgattctcgc cgctgactcg ctccgctcta taatcgatgt ccatgccgct cgaatggaat
    53581 gtcactgtcg ccgcaatgga aacggacgac tggagtgctg ttcgtgccat ctacctgcag
    53641 ggcataacaa ccgggaatgc cacgttcgag aaatccccgc ccacttggga gcagtgggac
    53701 agcagccatc tgacggtgtg cagattcgtg gcgcggtcgc agggcgaagt gcttggatgg
    53761 gcggcgctca gcccagtctc cggacgctgt atttatgccg gggtcgcgga ggtgagcatc
    53821 tacgttgcga acgggacgca gggcagaggg atcggactgg cgctcttatc cactctggtc
    53881 tcagcttccg agcaggctgg catctggaca ctgcaagcgg gcatttttcc ggagaacacg
    53941 gctagcatcg cactgtgcaa gcgggccgga ttccgaaccg tggggacgcg cgagcgttta
    54001 ggctgcatgg aaggccgctg gcgcgatgtg gtcctcatgg agcgcaggag cagcgtcgta
    54061 ggcatctgat tccttacaga tcattggcgt gcaatccgtt cgcgctccca ggctcccttt
    54121 cccccttccc acttggaaac agccctcgct ccaaacccga ttgcgcacta tgattgatcc
    54181 ctgatggaac gtagaatcct aaagtgtcaa tctatggcgc gggagcaccg ggcggaacat
    54241 gcaatcaccc ggcgccacct gttcgggacc atgacgggcg cagtagctgc cgcctggccg
    54301 cgggcggcag tccccgccga gacgaaaggt gacccccgct tgaccgcctg gatcttcatg
    54361 cagagtccgc tcgaacggtg gatgccggac ttccaccgta ccttcgatgc gtgggaggac
    54421 ggtggcgttc gcggcatcgc cctcggctac atgcgattcc aacgcgagaa cggcacggtt
    54481 ccgagctttc cgtcgaatcc ggacgtctac cgtgccttcg gcgtcagccc gccgcccgct
    54541 taccctcacg acccagcgaa ggccaggcaa tttcacgcaa tgctcgacga cgccgcaaag
    54601 cgcggttggg agatcctgct gttcggactc ggccggctgg gcggttcgcg gccgcccgcg
    54661 gaggatccgt tcgacgccgc tggtttcgcc gccaccgtcc aggacgccat caattactac
    54721 ccgcaggcga acggcatcat catcgacggc ccgggagagc accactatga gctcgcgttt
    54781 catcacggcg gcgagttgct cgaaatccgc gactccgaga ggccccgcct ggaacttctc
    54841 ggattcgatc ttgcccgcct cgagaagggg atcgcgcacc tgcgctcacg cctgcatcag
    54901 ctcacgcccg cgctcgtccg gtaccactcg cctggcggcc ttatcgctga tctcgcgctc
    54961 tttgatgtga acgaagacgt cctttactgg cttcgtacgc gccaggacgt ggccttgcgg
    55021 cacatggccg ctatccgaaa gcagatcgac gggttgaacc gcaaggtcaa actgggcgga
    55081 attccgcgga cggctgtctt ctccgcccaa accgctcagg attacgttcg gatgtcgccg
    55141 tatttcgact actggttccc gaagcactac ttctggcacc gcggcttcga cggcatgtac
    55201 ggcaccatcg cgcgctgggt tcagaccatc aaagaatgga atcctatttt aacggaggag
    55261 gattgtttcg ccgtcgtgaa gtcgtggttt ggaatcgagc ttcccggcgt tcactcactg
    55321 gccgacctgg agatgggctt tcccgatgag ttcttctccc gcgtggtcta ttccgaaacc
    55381 cgccgtgcgc tcgaagcagt tggcgatccc gacaaagtga tcgcctgggt ctcgaccggc
    55441 cgccttcctc acgccggcga cccaatgccg gcacgagacc tccacaacat tctgcttgca
    55501 tcgcggaagg cgggcctccg gcggtttctg ttccaccccg atccaaacct cggcgcagcg
    55561 gaatggagcg ttatctcatc gatgtgcggc aagcggtgga ttgaagatcc gtcaggctac
    55621 tggccgccgg acaccccgag gccggactcc ttcaacgggg cccgaaaacg cccgccgcgg
    55681 tgaccgcgtc acaagcgaat ccgctttcca tcaccgcgtg agcttcttca cggcaaactc
    55741 cagtcggacg tcctgtagaa gctctcgcat tcgttcgaac gtgcgccagt cggtgagatc
    55801 ctcggtgcgg aggcggaacg tagattcgag cagggcaggg gaatcgagaa agatgctctt
    55861 ggtcagcggc tcgtagcccg gtacggtcac gcgaatgcgt gccgtcgccc gagcctctaa
    55921 ccggaacgag ccgtccacaa aatcagcggc cgtttccgca atcgcctcct tgcccaccat
    55981 ccggatcacc tcccatctcc ctctcagcgg tcctccggta atgccgtcct tcacaactgc
    56041 ggtgatccga gcgggcacgg gctctggcgg acgcgagccc tccggctcaa agtaaaccgg
    56101 gttggtgatc gcgatgtgcc cggcatcagc cccaaagcag cgtgctatgt accacgctcg
    56161 ttcacgctcc accacgttga aacccgattc gatctcggtt ctcccgctta cgggctcgaa
    56221 cgtcttcaca accactccat tccggatcag ttccaccttc cggagacccg atgttgctgc
    56281 cccggacgcc catgccctga tcctgccttt gatggagacc aggcatggcc ctcctccgca
    56341 cgtcggtatg cccaccacgt cgccacttcc gtagccgttc atctccagca tcaccagcgg
    56401 gccgcttgtc actaaactcc gtcctgtctt gagcgcttcc gcaaccgcag ccaggctaaa
    56461 cccgccccgg actcgcgtgt acatccgaac tcttcccgga acgcctccac ccggccggtc
    56521 gaacgtcgca tccgtcgagc cactcgcggc aatgcggtag cccttgttca aaagcaggaa
    56581 ccagagttgc tgtgcgcaat cattcgcggc ggtttcatgc ggctgcatca ggacgtcgat
    56641 cgtgtcgtac gtcggaccgg ccaccgtgtc gaagggcagc tccgctgcca tgttggacac
    56701 gaacttcttt tcctcgaccg gatagatccc acgcccgccc cactttcccc accaccagcg
    56761 gcacggatgg ctgtaggaga ccacaccgcc cagtgaatga atcagcgcgt ggctttcgaa
    56821 gtttgccgcg ggatccttgt cgctctcgta atcgtgagca ctcagtgcga gcaattcgcc
    56881 gatggcgtcc cggccatcct cggtgcgccc gcgcatgccg gctgtccagc catgcccgag
    56941 gcacttcgag acgtcgccgc ggtaatagtt cttcggcgcc tcgaggttcc aggtcagcag
    57001 gaagtcgtcg gtgcttgcgt tccggcactc tcggtccagg tcttccggag tcgcttgggg
    57061 aaggttccag agatgcgcga gcgagagata atcaagccct tccgacctcg ctgccagggc
    57121 ggcgtaagcg aaatccacag tgaccatccg ctcgccgtgg accatgtggt catggttgtc
    57181 gccggtgtac cacccttcca tccgaagcgg ggttcgcctt ttcagttcat aggtcagttc
    57241 gcgccgctct ccggcacgca gcacgaactt ccgttccaca ga
//
LOCUS       DATIFQ010000505         4094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2471703, whole genome shotgun sequence.
ACCESSION   DATIFQ010000505 DATIFQ010000000
VERSION     DATIFQ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471703"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VLE22_25555"
     CDS             complement(<1..893)
                     /locus_tag="VLE22_25555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17841.1"
                     /translation="MRRIHLKAIACLSLIGVLFYWKFLLTHQYSLLTGFEGANQAYSW
                     YGYIARSIKEGSWPIWDPYTFSGRSFVGEMQTAAFYPINFLLAAIPFNDRGLLSPGFY
                     HWLYLAAHVLGAYFTFALVRTFGLSVFASLLSGICFSFGGFMARLGGWPHMFQSGIWL
                     PLICLFTVKALNEVDSRRRLAYSVLGGLGLGLSILAGGLHLAMMQAAVVTTMSIAFIF
                     LAGKGRGRELWKRSILVFAVVLIIGTISGAVQILPSIEYSQLSFRSITGGSVGASDRI
                     DYQQLNHGLFANALSGLVFGAP"
     gene            1102..3660
                     /locus_tag="VLE22_25560"
     CDS             1102..3660
                     /locus_tag="VLE22_25560"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HSB17842.1"
                     /translation="MKRTALRLAVLALAVTGCTWAADDDETGRGVARVSLINGDVSVR
                     RGDSGDWVAAAVNAPLVVEDTVSTGPSSRAEVQFDWANMLRLASNAEVRLAQLDYRRY
                     LIQIARGTVTFRVLRDSEADVELSTPSVSVRPVRKGTYRITVREDGESEITVRSGEAE
                     VFTPRGSERLKSGRTMMARGTASDPEFQIAREIPDDSWDDWNVSRDRYLERAQGYRYA
                     NRSVYGIEDLDGYGSWDYVAPYGYVWAPRVAVGWSPYYYGRWSWIDWYGWSWVSYDPW
                     GWAPYHYGRWFWHGSRWCWWPGGYGSRHHWSPGLVAFFGYGHGGSHFGIGFGNVGWVP
                     LAPYEPYHRWYGSGYYRGYRNNTYIDNSVTIVNNTNIYNTYRNARVNNAVTAVDASSF
                     GRGNGNLVRVRSEELNRAGLVKGVLPVAPGQESLRVSNREVNRAALPREVNPGRFYSR
                     TAAPRVDRVPFEDQRRGMERVAQRTFGETAGRPAESVRATGGAVTDDARRGGAVRTPE
                     TVGRPAESVRATGGAVTDDARRGGAVRTPEGSIVRNPEQRGNTGWRRIGESPAAETRG
                     GATRTEGGVRNTPSDVRRGETPAESGVRSTPGEVRRGGTPAESGVRSTPSEVRRSGET
                     DRGGWRRFGEPGRQSNTGGEAAAPRVMDRTGGRNDEVRIQGGNRGEAVRSTPSEVRGS
                     DSERGSWRRFENQRNEPQRMETPRSETPRDNSSRYESFGSRSPRAERNSEDRSIRVSP
                     PIVRERETGRFDSGGFGEPRRYEAPRTESPRMDTPRYESPRYESPRMNAPRYEAPRSE
                     SPRMSAPRSEAPRYEAPRMSAPRGGGEMRAPQVSRGNDGGGRSSRGEGRGGRGR"
     gene            3852..>4094
                     /locus_tag="VLE22_25565"
     CDS             3852..>4094
                     /locus_tag="VLE22_25565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17843.1"
                     /translation="MITMIVRTAAAGLALAALALGQAPQQQQPVVNPAPAASELADVL
                     TRHVSEDLRVKAAVGKPVTAGSVTLIPILMIDINFGG"
BASE COUNT          816 a         1197 c         1429 g          652 t
ORIGIN      
        1 ggggcgccga acaccaatcc agacaacgca ttcgcaaaca ggccatggtt cagttgctgg
       61 taatcgatcc tgtctgaagc gccgaccgag ccgcccgtaa tgctgcggaa agaaagctga
      121 ctgtactcga tggacggaag gatctggacc gcgcccgaga tcgtgccgat gatgaggacg
      181 accgcgaaga ccaggattga tcgcttccac aactcccggc cgcgaccctt gccggcgagg
      241 aagatgaatg caatgctcat cgtcgtcaca acggcggcct gcatcatggc gagatgaagt
      301 cctccggcaa ggatcgagag accgaggcca agtccaccca agaccgagta agccaatcgc
      361 ctgcgagaat cgacctcgtt gagagcctta accgtgaaca gacagatcag cggaagccag
      421 atgccgcttt ggaacatatg cggccatcca cccagccttg ccatgaatcc accgaaagag
      481 aagcaaattc ccgacaggag tgacgcgaac acactgaggc cgaaggtgcg gactaatgca
      541 aacgtgaaat acgcccctaa tacatgagcc gcaaggtaca accaatgata gaaacctggc
      601 gacaacaggc ctcgatcgtt aaacggaatc gccgccagca ggaagtttat aggatagaac
      661 gccgctgtct gcatctcgcc cacgaaggag cgccccgaga acgtgtacgg atcccagatg
      721 ggccaactac cttcctttat gctgcgggca atatagccat accaggaata ggcctgattc
      781 gccccctcga aacctgtcag gagcgagtat tgatgtgtca gcagaaactt ccagtagaac
      841 agaacgccga tcaggcttag acaagcgatg gctttcaggt gaattcggcg cattacggct
      901 cccaaaaggg gatgccgccg gctggaaatt cgccggcagg gccggtgaga tgcaccagcc
      961 agcgtggtcg atcccgatcg gtgtggtgct tttcggccac ctgcataggt agccatcata
     1021 tcgggaatcc tcacaaaatc aaagacttcc ggtttggttc acaagttgca ctaatgaggt
     1081 cggagtttta ggaggcgcgt catgaaacgg acagcgttgc ggttagcggt tctggcactt
     1141 gcggtgaccg gctgcacctg ggccgctgat gacgacgaaa cgggtcgggg tgtggcccgc
     1201 gtgagtctga tcaacggcga cgtctcggtg cgccgcggag attccggcga ctgggtggcg
     1261 gcggcggtca acgcgccgct ggttgtggaa gacaccgtct cgacggggcc ctcctccagg
     1321 gcggaagtgc agtttgactg ggcgaacatg cttcgccttg cctccaatgc ggaagtccgg
     1381 ctggcccagc ttgactaccg gcgctacctc attcagatcg cgcggggaac ggtgacgttc
     1441 cgcgttctgc gcgactccga agcggacgtt gagttgagca ccccgagcgt ttccgtgcgt
     1501 ccggtgcgca agggaacgta tcggatcacg gtgcgggaag acggcgagtc cgaaatcacg
     1561 gtgcgctccg gcgaggccga ggtgttcacg ccgcgaggtt ccgagcgttt gaagtccggc
     1621 cgcacgatga tggcgcgggg caccgcgtcc gatccggagt ttcagatcgc gcgcgagatt
     1681 cccgacgaca gttgggacga ctggaacgtc agccgcgacc gttatctgga gcgcgcgcag
     1741 ggctaccggt acgccaatcg cagtgtgtac ggcatcgagg accttgacgg ttacggatcg
     1801 tgggactatg ttgcgcctta cgggtacgtc tgggcgccac gcgtggcggt cggctggagc
     1861 ccgtattact acggccgttg gtcgtggatc gactggtatg gctggagctg ggtgagttac
     1921 gacccctggg gctgggcgcc gtatcactac ggccggtggt tctggcatgg gagccggtgg
     1981 tgctggtggc cgggcggcta cggcagccgc catcactgga gcccgggcct ggtggcattc
     2041 ttcggttacg gacacggcgg gtcgcacttc ggcatcgggt tcggtaatgt cggctgggtg
     2101 ccgctggcgc cgtatgagcc gtaccatcgg tggtacggaa gcggctacta tcgcggctac
     2161 cggaacaaca cgtacatcga caacagcgtc acgatcgtca acaacacgaa catttacaac
     2221 acctatcgga acgcgagggt gaacaacgcc gtgacggcgg tggacgcctc cagtttcggg
     2281 cgcgggaacg ggaatctggt ccgggtacgc agtgaagagt tgaaccgcgc gggcctggtg
     2341 aagggcgtgc tgccggtggc gccgggtcag gaaagccttc gggtgtccaa ccgggaggtc
     2401 aaccgggcgg cgttgccacg cgaggtcaac ccgggccggt tctacagccg gaccgcggcg
     2461 ccgcgcgtgg atcgcgtgcc gtttgaagat cagcggcgag gcatggagcg ggtggcccag
     2521 cggacgtttg gcgagaccgc tggacgcccg gcggaatcgg tccgtgcgac gggcggagct
     2581 gtgaccgacg atgcacggcg cggcggtgcc gtgcggacgc ccgaaaccgt gggacgcccg
     2641 gcggagtcgg tccgtgcaac gggcggagct gtgaccgacg acgcacggcg cggcggtgcc
     2701 gtgcggacac ctgagggaag catcgtgcgg aaccccgagc agcggggcaa cacgggctgg
     2761 agacggatcg gcgaatcgcc cgcggcggaa acccgcggag gcgcgacccg gacggaaggc
     2821 ggagtccgta acacaccgag tgatgtgcgc cggggtgaga cgcccgcgga gagcggcgtc
     2881 cggagcacgc cgggtgaagt gcggcgaggc gggacgcccg cggagagcgg agtccggagc
     2941 acgccgagtg aagtgcgccg gagcggggaa acggatcgcg gcggctggcg ccggttcggg
     3001 gagccgggcc ggcaatccaa cactggcggg gaagctgcgg ctccccgggt gatggaccgc
     3061 accggtgggc gcaacgacga agtccgcata cagggcggca accgcggcga ggctgtacgc
     3121 agcacgccgt ctgaagtgcg agggagcgac tccgagcgcg gaagctggag gcggtttgag
     3181 aaccagcgta acgaaccgca gcgaatggaa acgcctcgga gcgagactcc gcgggacaat
     3241 tcttcccggt acgagtcgtt cgggtcaagg agcccgcgcg cggagcggaa cagcgaagac
     3301 cggtccatcc gggtgagccc tccgatcgtg cgggagcgcg agaccggaag gtttgacagc
     3361 ggcggattcg gtgagccgcg ccggtacgaa gcgccgcgaa ccgagtcgcc gaggatggac
     3421 acaccgaggt atgagtcgcc gaggtatgaa tcccctcgga tgaacgcgcc gcggtacgag
     3481 gctccgcgga gtgagtcacc tcggatgagc gcaccacggt ccgaagcacc ccgctatgag
     3541 gcgcctcgca tgagtgcacc gcggggcggt ggtgaaatgc gcgcgccgca agtgtcgcga
     3601 ggcaacgatg gcggcggccg gtccagccgt ggcgaaggcc ggggaggccg cggccggtag
     3661 cacagcctga gggctgcgcc actcggctga caacgatctt cttccctcca tggcccgcat
     3721 cggggaaacc cggtgcgggc catttttttg tctgcgccga gcttgttcga atggtgttga
     3781 gcgtattcca gccgtgcggt ggtataaatc gagattcgcc caaaccgcca cagttacaca
     3841 ggaggtgttg tttgataaca atgattgtcc gaaccgctgc ggccggcttg gcgctggccg
     3901 cccttgctct ggggcaggct ccgcagcagc aacaaccggt ggtgaatccc gccccggccg
     3961 cgagtgaact ggccgatgtt ctgacccggc atgtcagcga agacttgcgc gtgaaggcgg
     4021 ctgtcggcaa acccgtcacc gccggctctg tcacgctcat ccccatccta atgatcgaca
     4081 tcaacttcgg cggc
//
LOCUS       DATIFQ010000506        10853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1132423, whole genome shotgun sequence.
ACCESSION   DATIFQ010000506 DATIFQ010000000
VERSION     DATIFQ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132423"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..538
                     /locus_tag="VLE22_25570"
     CDS             146..538
                     /locus_tag="VLE22_25570"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSB17844.1"
                     /translation="MSFTVFLGNLPTWVTADDIHSWLSAESLVADAIKVIRNPETQES
                     KGFAFIETPNSEEMQSIIRRFDRAPLEDRLLRANPAQPPKSKGAPLVRPTTAQRPVHR
                     AGKKHVKRGRPDQQSPQSAFAEELAKAL"
     gene            706..1791
                     /locus_tag="VLE22_25575"
     CDS             706..1791
                     /locus_tag="VLE22_25575"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSB17845.1"
                     /translation="MKDLLLIRTLFVLLLTCASLFIHPFGIPSPWSGAIGLALGLVIV
                     FFEIRVKEVSLKRLIGAAVGSVLGIFGAFLMSLVLSRALPNNPDTVPFVQIGLLLWMT
                     YVGLVVGASKGDMLNLSALGGIFGGEKIGKQTFKILDTSVIIDGRIADIAETGFLDGI
                     VVIPQFVLRELQLVADSADSMKRNRGRRGLDILQRIQKMPQLNVQILEDDFPHIRDVD
                     MKLIELAKTYDCKIVTNDFNLNKVAQLHGVEVLNINELANALKPIVLPGEVMRVFILK
                     EGKEYNQGVAYLDDGTMVVVDNAKRMISKTIDISVTSVLQTTAGKMIFGRYDEKIHAI
                     HEKAPSERHERIHKEQQAGAAAMPPTR"
     gene            1851..2564
                     /gene="ispD"
                     /locus_tag="VLE22_25580"
     CDS             1851..2564
                     /gene="ispD"
                     /locus_tag="VLE22_25580"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HSB17846.1"
                     /translation="MILPAAGLGTRMGRTSPEKAGTSRKQFMLLDGSPILLHTIRKFV
                     SCALVSEIVVALRSEDKAWVNEMLTAEALPKPVRLVEGGDTRQQSVENALTTLSPDTD
                     LVSVHDAVRPFIDPAIIDKVIREAADSGAAIVGIVPVDTIKQVRRNKIRTTLPRERLV
                     LAQTPQVFRFELLQRAFAMAREDGFIGTDESSLVERLDQVEVSVVPGTDRNIKITKPS
                     DMDLARLFLAEQSAERKVS"
     gene            2561..3046
                     /gene="ispF"
                     /locus_tag="VLE22_25585"
     CDS             2561..3046
                     /gene="ispF"
                     /locus_tag="VLE22_25585"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HSB17847.1"
                     /translation="MSEYRTGLGWDVHRLASGRPLILGGVRIPSEFGCDGHSDADVLA
                     HAVTDALLGAAALGDIGMHFPDTDPRWAGADSLQFLRHARDLAAGAGYEVVHIDTNVI
                     LERPKLKDYRLAIRESLAAAVGLDLDRVSVKFRTAEKVGPVGEGRSAEAQAVVTLRRA
                     G"
     gene            complement(3053..4609)
                     /locus_tag="VLE22_25590"
     CDS             complement(3053..4609)
                     /locus_tag="VLE22_25590"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSB17848.1"
                     /translation="MQRILLSIAIASAAAAQSPVIDGSRLRADTKFLASDLLEGRGVG
                     TRADRLTTEYLATQFALIGAKPAGDNGSYFQRVPLVGVETQPGAQLTAAGGGKTLSLK
                     WLDDFVGVSQRQLADQQFDAEAVFVGHGIVAPEFGWDDFKGVDVRGKIVVLFTNEPPS
                     DDDNLFGGKALTYYGRWTYKYEQALRAGALGAVILHTTPTAGYGWNVVRNSWGREEPY
                     VKLKPGEPALAFAGWINQQAGERLAAMAGNTLETLEKRANSRDFRPFSLQFRLRGRMP
                     AKVRELDTRNVAAIIEGSDPGRKSEAVLFTAHWDHLGIGAPVGDDNIYNGAVDNATGC
                     AILLEIARAWAALDPKPRRSALFLAVTAEEGGLRGSEFYAANPLVPIEKTPIALNFDG
                     YYPFGRTKDVILNGAERTKLWPLIQDVAARMDLKILPDPRPEQGSYFRSDHFPLAKAG
                     VPAFSISMGKEFYGKPEGFGEKLFEEYNSKHYHQPSDEYSEDWDFAGIEHIARFGFTL
                     GLEVANMEKR"
     gene            4711..5295
                     /locus_tag="VLE22_25595"
     CDS             4711..5295
                     /locus_tag="VLE22_25595"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSB17849.1"
                     /translation="MADESKPADKPAPASGGHAAPKPAAVMAATPWESDLTRQLKEQF
                     GDRILEFSTYLGQEFLVATPEAAIPILEYLKLEAEFDYLVDVTAADFPSRPERFDVIY
                     ILYSFARNQRIRIKIRIPEGFKPRTAVGVHLTADWLEREVFDMFGIEFEGHPDMRRIL
                     LPEEWQGYPLRKDNPITQQDERWVKENLGIDSAQ"
     gene            5302..6414
                     /locus_tag="VLE22_25600"
     CDS             5302..6414
                     /locus_tag="VLE22_25600"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSB17850.1"
                     /translation="MADSTFLDTTELVLNMGPQHPSTHGVLRVILKLDGEKVMGTKCV
                     IGYLHRGVEKIGENRTYQMFAPYVDRMDYVAAVCNGLGYCLTVEKLLNVEAPPRAQAV
                     RVILTELNRIASHLVWLGTHALDIGAITPVFYTFREREYTLDIFEKYCGARLTTHAFR
                     IGGLQYELYDGFEEDVKKFCKLFLPKVDEYEELLSGNRIWKGRLTNIGVLNAEDCKAY
                     GVTGPMLRAAGVKWDIRKAKPYSGYEKYNFEIPTRENGDTYDRYIVRMQEMRQSTRII
                     QQAVEDIPSGPIMAKVPKVIKPPVGEVYVSTEAPKGELGYYIVSDGSPQPYRVRVRPP
                     SFVNLQSLDKMARGGLIADMVAVIGTIDIVLGEVDR"
     gene            6448..6903
                     /locus_tag="VLE22_25605"
     CDS             6448..6903
                     /locus_tag="VLE22_25605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HSB17851.1"
                     /translation="MRKFLRTVFLVDLVQGLWVTFRYQRPKHICTEQYPAQRPKVAER
                     YRGAPRLNINPDTGETLCISCNLCALACPENLIVVTSERNEQTRRKDLTTFTYDISRC
                     MFCGLCEDACPVDALELTQDFEMAFYTREGAIWDRQMLEEGPKPTRYKF"
     gene            7048..7557
                     /locus_tag="VLE22_25610"
     CDS             7048..7557
                     /locus_tag="VLE22_25610"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSB17852.1"
                     /translation="MIDTIFFYVFALLVLGGGILTITRRSAVHSAIWLTVSLLGVAGL
                     YLLQKAEFLFAVQIVLYIGGITLLYLFVIMLVNLDEAARQRQFNKQWWLAVACVLAVG
                     AEVFYFISKGLGAFQLARPGEASPVTEGNTERLADMLFSDYLLPFEVASVLLLVAIVG
                     SVVMAKKRI"
     gene            7557..7868
                     /gene="nuoK"
                     /locus_tag="VLE22_25615"
     CDS             7557..7868
                     /gene="nuoK"
                     /locus_tag="VLE22_25615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004321.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSB17853.1"
                     /translation="MFHPVTTVHYMVLSAALLMIGTAGVLARRNIVIILMSIELILNA
                     VNINLVAFSHYFRSVSGQIFAVFVIAVAVAEAAVGLGILIALFRNKETVNADEVDLLK
                     W"
     gene            7879..10029
                     /gene="nuoL"
                     /locus_tag="VLE22_25620"
     CDS             7879..10029
                     /gene="nuoL"
                     /locus_tag="VLE22_25620"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSB17854.1"
                     /translation="MSFLDNIWLIPLFPLFGAAAMLLLGKRLDPQAPSDVAVAPGAEP
                     SPAPPHGHHGSHSSGKKALISLLCPGMVLVSLVFSIGAVVELAGKAEKIHEVIKYAWM
                     AGLPFRMADGTMARFGADFGFLLDPLSSVMILVVTGVGFLIHVYAVGYMGHEGGYYRF
                     FGYMNLFVFFMLMLVLANNYLLLFVGWEGVGLCSYLLIGFYFHKKSAGDAANKAFIVN
                     RIGDAGFILGMLLMFGVLGTVRFVDVNAALTSGRFAAETAGFGVLSLMALLLFVGATG
                     KSAQFPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMVARSSALFSLTPETSMVVAAI
                     GAFTAILAASIALVQNDIKRVLAYSTVSQLGYMFVACGVGAYWVAVFHLFTHAFFKAL
                     LFLGSGSVIHALSGEQDMRKMGALKDKIPVTHWTMFIASLAIAGIPGFAGFFSKDEIL
                     WQAYSSPLGSKVIFAVGLITAGMTAFYMWRLMYLTFYGKSRVEPAAAPHIHESPRVMT
                     IPLSVLAVGSVLAGWIGVPKLWSVFPDAFRTFEHWLAPALASASAHVAETAHHDAATE
                     WLLMLLSVAVALAGIWLARYMYDIKPEKADALASSAPGLYNTLLNKWYVDEIYGFLFI
                     DGMAKGGGTVLGTFDNRVVDGGVNGAGWLTRLTSTVSIWWDTWIIDGSVRLMSFLVKL
                     SSYPMRILQTGQFQAYALAFVLGVLIFFGYYMMR"
     gene            10034..>10853
                     /locus_tag="VLE22_25625"
     CDS             10034..>10853
                     /locus_tag="VLE22_25625"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSB17855.1"
                     /translation="MNEHLLSIVLFTPLAGMIVLLFIPAAQKNLIRVWANLVALAGFL
                     VSIPLVMRYDRTSAGFQMIEKFDWIPALGAKYYVGIDGISLLLVMLTTLVGFLAILSS
                     WSAVEDRLKSYYAMFLLQQTGMIGVFLALDFFLFYVFWEVVLVPMYFIIGVWGGPRKL
                     YAAIKFFLYTLAGSVLMLLGILTLYFQHFRQFGFYTFEIADLMKLDLPLGLQQWVFWA
                     FFIGFAIKVPMFPFHTWLPDAHVEAPTAGSVILAAVLLKMGTYGFLRFSLPLLPK"
BASE COUNT         2006 a         3216 c         3425 g         2206 t
ORIGIN      
        1 cgcggcgtcc ccggcctctg accaacccgc ctggaagcct ttttgtttgc aaagggccgc
       61 tccctctgtt aagatcaagg agcaggaatg gcacggttcc tctcgccagt ctctcccgca
      121 atcccgaaaa cggagtgagc atcagttgag ttttactgtc ttccttggca accttcccac
      181 ctgggtcacg gcggacgaca ttcattcttg gctaagcgcc gagagtctag tcgccgacgc
      241 tatcaaggta attcgcaacc ccgaaacgca agagtcgaaa ggcttcgcat tcattgagac
      301 cccgaactcg gaggagatgc aatcgatcat ccgccgtttt gaccgggcgc ccctggagga
      361 ccgccttctg cgggccaacc cggctcagcc acccaagtca aagggtgctc cgctggtgag
      421 acccaccacc gcgcagcggc ccgtgcatcg ggcaggcaag aagcacgtca agcgcggccg
      481 gcccgatcag cagtcgccgc aaagcgcgtt cgcggaagaa ctcgccaaag ccctctaaag
      541 tcatccgaac cgtccgaaag catcccgcac cagctctggc gccgccgcga gggagtcctt
      601 ctcccttccg ggcgtgcctc ccgcatcggt ggtatactca atttcgcggg acattatatt
      661 tggcatcttc aagactaaca gccccgtgaa acgagggttg atctcgtgaa agacctcctc
      721 ctcatacgta ccctgtttgt ccttttgttg acctgcgcca gccttttcat tcatccattt
      781 ggcattccct cgccgtggtc cggagcgatt gggctggctc tcggtctggt cattgtgttt
      841 ttcgaaatcc gggtcaaaga agtaagcctg aaacggctga tcggagcggc cgtcggttca
      901 gtgctgggca tcttcggagc gtttctgatg tccctggtgc tgtcgagggc cctgccgaac
      961 aatccggaca ccgtaccttt cgttcagatc ggacttctgc tgtggatgac ctacgtgggc
     1021 ctcgtagtag gcgccagcaa gggtgacatg ctgaacctct cggctctcgg gggcatcttc
     1081 ggcggagaga agatcgggaa gcaaaccttc aagattctcg acacgagcgt gatcatcgac
     1141 gggcgcatcg ccgacatcgc agaaaccggg ttcctggacg gcatcgttgt gatcccgcag
     1201 ttcgtcctgc gggaattaca gcttgttgcc gattcggcgg actcgatgaa acgtaaccgg
     1261 gggcggcggg gcctggacat ccttcaacgg atccagaaaa tgccgcagct caacgtgcag
     1321 atcctcgagg acgactttcc gcacatccgc gacgtggaca tgaagctgat cgagctggcc
     1381 aagacctacg actgcaagat cgtcaccaac gatttcaact tgaacaaggt ggcgcaattg
     1441 cacggcgtcg aggtgctcaa catcaacgaa ctcgctaacg cgttgaagcc gatcgttctg
     1501 ccgggcgaag tcatgcgagt ctttatcctg aaggaaggta aagaatacaa ccagggagtg
     1561 gcttacctgg atgacggcac catggtcgtc gtggataacg cgaagcggat gatctcaaaa
     1621 accattgaca tctccgtgac cagcgtgctt cagacgaccg ccggtaagat gatcttcggc
     1681 cgatacgacg agaagatcca cgccattcac gaaaaggcgc ccagcgagcg gcacgaacgc
     1741 atacacaagg agcagcaggc gggtgcagcc gccatgcctc ccaccaggta gcacgggggc
     1801 gagcgggacg ccgccaccag cgtgacatcg acaagaccat gaaggtagcc gtgatcttac
     1861 cggccgcggg gctaggtacc cgcatgggcc gcacatcccc agaaaaagcg ggcacgagcc
     1921 gcaagcagtt catgctgctg gacggctctc cgatccttct gcacacgatt cgtaagttcg
     1981 tttcgtgcgc gctggtgagt gaaatcgtcg ttgccttgcg ctccgaagac aaggcgtggg
     2041 taaacgagat gctgaccgct gaggcgttgc ccaagccggt tcgactggtg gaaggcggcg
     2101 acacccgcca gcaatcggtc gagaacgccc tgacgacgct ctctccggac accgacctgg
     2161 tctcagtgca tgatgctgtc cggcctttca tcgatccggc gatcattgac aaagtgatcc
     2221 gtgaggccgc cgattccggg gccgccatcg tcggtatcgt accggtggac accatcaagc
     2281 aggtgcggcg gaacaagatc cgcaccacgc tgccacgcga gcggctggtg ctcgcacaga
     2341 ctccgcaggt cttccgcttc gagcttctcc agcgcgcatt cgcgatggcg cgagaggacg
     2401 gcttcatcgg aacggacgag tcgagcctcg tcgagcgtct cgaccaggtc gaggtgagcg
     2461 tggtgccggg caccgaccgc aacatcaaga tcacgaagcc gtccgacatg gaccttgccc
     2521 gcctgttcct ggccgaacag tccgccgagc ggaaggtgtc gtgagtgaat accggaccgg
     2581 cctcggctgg gacgtccatc gtcttgcatc cggccgtccg ctgattctcg gaggcgtgcg
     2641 gatcccgtcc gaattcggat gcgacggcca ctccgacgcc gacgtgctgg cgcacgctgt
     2701 caccgacgcc ttgctcggcg ccgccgcgct cggggacatc ggcatgcact ttccggacac
     2761 cgatccccgg tgggcgggcg cggacagcct gcaattcctg cggcacgccc gcgacctggc
     2821 tgccggcgcg ggctacgaag tggtccacat cgacaccaac gtcattctgg aacgcccaaa
     2881 gctgaaggac taccggctgg cgatccgcga aagcctggcg gcggcggtcg gtctggatct
     2941 cgaccgcgtt tcggtgaagt tcagaactgc cgaaaaggtc ggcccggtcg gcgaggggcg
     3001 ctcggccgag gctcaggcag tggtcacgtt gcggcgggcc ggctgaattc cgctaccgtt
     3061 tttccatgtt cgcgacttcc aggccgagcg tgaacccgaa acgtgcgatg tgctcgatgc
     3121 cggcgaaatc ccagtcctcg ctgtattcgt cggatggctg gtggtagtgc ttcgagttgt
     3181 actcctcgaa cagcttctcg ccgaagccct ccggtttccc atagaactcc ttgcccatgc
     3241 tgatcgagaa ggcaggcacg cccgccttgg cgagcgggaa gtgatccgag cggaagtagc
     3301 tgccttgctc gggccggggg tcgggcagga tcttgagatc catgcgcgcg gcgacatcct
     3361 ggatcagcgg ccatagcttc gttcgctcgg cgccgttcaa aatgacgtct tttgttcgcc
     3421 caaacgggta ataaccatcg aaattgagcg ctatcggagt cttttcgatg ggtacgaggg
     3481 ggttcgccgc atagaactct gagccgcgca gcccaccttc ctcggcggtg accgccagga
     3541 aaagagctga gcggcgcggt tttggatcga gagcggccca ggcgcgggcg atctccagca
     3601 ggattgcgca gccggtggcg ttgtcgaccg cgccgttgta gatgttgtcg tcgccgaccg
     3661 gggcgccgat gccgaggtgg tcccaatgag cggtgaagag gacggcctcc gacttgcggc
     3721 cggggtcgct gccctcgatg atcgccgcca cgttccgtgt atcgagctcc cggacctttg
     3781 ccggcatgcg cccgcggagt cgaaactgga gtgaaaatgg tcgaaaatcg cgcgaatttg
     3841 cacgcttttc gagcgtttcg agcgtgtttc cagccatcgc ggcgagcctt tcgcccgcct
     3901 gctggttgat ccatccggca aacgccagcg ccggctctcc cggcttcaac tttacgtagg
     3961 gctcctcgcg cccccaggaa ttccggacga cattccagcc gtagcccgcc gtcggagtcg
     4021 tgtgcaagat caccgctccc aacgcacccg cgcgcaacgc ctgctcatat ttgtaagtcc
     4081 aacgtccgta atatgttaga gccttcccac cgaagagatt gtcgtcgtcc gacggcggct
     4141 cgttggtgaa cagcacgaca atcttgcctc ggacgtcgac ccccttgaag tcgtcccagc
     4201 cgaactcggg cgcgacgatg ccgtggccca cgaaaaccgc ttcggcgtcg aactgctggt
     4261 cggcaagctg gcgctggctg acacccacaa aatcgtccaa ccatttcaag ctcagggtct
     4321 tgcctccgcc agccgcagtg agctgtgcgc cgggttgcgt ctcgacgccc accagcggca
     4381 cgcgctgaaa ataggagccg ttgtcgccgg ccggcttggc gccgatgagg gcgaattgcg
     4441 tggcgaggta ctcggtggtc agacggtcag cacgagtacc gacaccccgg ccttccagga
     4501 ggtcactggc aaggaatttc gtgtcggccc gcagtcggga gccgtcgatg accggggatt
     4561 gggccgcagc ggccgatgcg atggcgatcg agagcaagat cctttgcatg agatgacctg
     4621 agggttccag tgtagcaatg gcggctggcc ggcccaattt gacaccccag aagagcgcct
     4681 tgtaagctgg tgattctctc gatttctcat atggctgatg agtccaaacc cgcggacaag
     4741 ccggcgcctg cctccggtgg ccacgccgcc cccaagcccg ccgcggtgat ggccgccaca
     4801 ccgtgggaga gcgatctcac ccggcaactg aaagagcagt tcggcgaccg gatcctggag
     4861 ttctccacat atctcggcca ggagttcctc gtggcgacgc ccgaagcggc cataccgatc
     4921 ctcgagtatc tgaagctgga ggcggaattc gactacctgg tggatgtgac cgcggccgat
     4981 ttcccctccc gccccgagcg attcgacgtc atctatatct tgtatagctt cgctcggaac
     5041 cagcgcatcc gaatcaagat ccgcataccg gaaggcttca agccgcgaac cgccgtgggg
     5101 gtccatctga cggccgactg gctcgagcgg gaggtcttcg acatgttcgg catcgaattc
     5161 gaaggccatc cggacatgcg ccgcatcttg ttgcccgagg agtggcaggg ctatccgctt
     5221 cgcaaggaca atccgatcac gcagcaggac gagcgttggg tgaaggagaa cctgggtatc
     5281 gacagcgccc aataaaccca catggcggac tccacctttc ttgacaccac cgaactggtc
     5341 ctgaacatgg ggccgcagca cccgtccacc cacggcgtgt tgcgcgtcat cctgaaactg
     5401 gatggcgaaa aggtgatggg cacgaagtgc gtcatcggct accttcaccg gggcgtggag
     5461 aagatcggcg agaaccggac gtaccagatg ttcgcgccgt acgtggatcg gatggactac
     5521 gtggcagcgg tctgcaacgg gctcggctac tgtctgacgg tggagaagct gctgaatgtg
     5581 gaagcgccgc cgcgggcgca ggctgtgcga gtgatcctga cggaactgaa ccggatcgca
     5641 agccacctgg tgtggcttgg aacgcacgca ctcgacatcg gggccattac gccggttttc
     5701 tataccttcc gcgagcggga atacacgctg gacatctttg agaagtactg cggcgcgcgg
     5761 ctgaccacgc acgcgttccg gatcggcggg ctgcaatacg aactctacga cggttttgag
     5821 gaggacgtga agaagttctg caagctattc ctcccgaagg tcgatgaata cgaggaactg
     5881 ctgagcggga accgcatctg gaaggggcgg ctgacgaaca tcggagtcct gaacgcagag
     5941 gactgcaagg cttacggagt cacgggtccg atgctgcggg cggcgggcgt aaagtgggac
     6001 atccgcaagg cgaagccgta cagcggttac gagaaataca atttcgagat tcccacccgg
     6061 gagaacgggg atacttacga ccggtacatc gtgcgcatgc aggagatgcg gcagtcgaca
     6121 cggattatcc agcaggcggt cgaggacatc ccgagcgggc cgatcatggc gaaagtgcca
     6181 aaggtgatca agcctccggt gggagaagtt tacgtttcga ctgaggcgcc gaaaggcgaa
     6241 ctcggctact acattgtcag cgatgggtcg ccccagccgt accgggtgcg ggtgcgcccg
     6301 ccttctttcg tgaatttgca gtcgctggac aaaatggcgc gaggcgggct aatcgcggac
     6361 atggtggccg tcatcggtac aatagatata gtgctgggag aggtggaccg gtaagcagtt
     6421 cagccggttc ccggcgcggg aggcagtatg cgaaagtttc tcagaacggt gttcctcgtg
     6481 gaccttgtcc aggggctctg ggttaccttc cgctaccagc gtccgaagca catctgcacc
     6541 gagcagtatc ccgcccaacg gcccaaggtg gcggaacgct accgtggagc accacgcctc
     6601 aacattaatc ccgataccgg ggaaacactg tgcatctcat gcaatctctg cgcgctggcc
     6661 tgccccgaga acctgattgt ggtgaccagc gagcggaacg aacagacgcg ccggaaggac
     6721 ctgacgacct tcacgtacga catctcacgc tgcatgttct gcggcttgtg cgaagatgcg
     6781 tgtccggtgg atgcgctgga gctgacccag gatttcgaaa tggcgttcta cacgcgtgaa
     6841 ggcgcgatct gggaccggca gatgctcgaa gaagggccga agccgacccg gtacaagttt
     6901 tagaagggtg ctgaagaacc tgttgcacgg cctgaacagg ctgctgaaaa agtttgccgg
     6961 gcgccgctcc ctcacggtcg cggttcctca agtgcccggg aaccgcacaa ctctgtggac
     7021 ggcgttcggt gcaccagcaa ctgagacatg atcgacacta tcttctttta tgtgtttgcc
     7081 ctgctcgtgc tgggcggtgg aatcctcacc atcacccgcc gaagcgcagt gcacagtgcg
     7141 atttggctga ccgtgtcgct tctcggggtc gccggcctct accttctcca gaaggcggag
     7201 tttctcttcg ccgtccagat cgtgctgtac atcggcggca tcacgttgct gtatctgttc
     7261 gtgatcatgc tggtgaacct ggatgaagcc gccaggcagc gccagttcaa caagcagtgg
     7321 tggctggcag tggcgtgcgt gctggcggtc ggtgcggagg tgttctattt catttccaag
     7381 ggcttagggg cgttccagtt ggcccggccc ggggaggcct cgccggtgac ggaagggaac
     7441 accgaacgcc tcgcggacat gctgttttcg gactacctgc tgccgttcga ggttgcttcg
     7501 gtgctgctgc tggtcgcaat tgtcggttcc gtcgtgatgg cgaagaagag gatctgatgt
     7561 tccacccagt cacaaccgtt cactacatgg ttctaagcgc ggcgctgctg atgatcggaa
     7621 ccgccggcgt gcttgcccga cgcaacatcg tcatcattct gatgtcgatc gaactcatcc
     7681 tgaatgcggt caacatcaac ctggtcgcgt tctctcatta cttccggagt gtcagcgggc
     7741 agatcttcgc ggtctttgtc atcgcagtgg cggtggcgga ggcggcggtg gggcttggca
     7801 tcctgatcgc gctcttccgc aacaaggaga cggtcaatgc cgacgaggtt gacctgctga
     7861 agtggtgagg actctgcgat gagctttctc gacaacatct ggttgatccc gctcttcccg
     7921 ctcttcggcg cggcggcgat gctgcttctg ggaaagcggc tggacccgca ggcaccctcg
     7981 gacgtggcag tggcgcccgg cgcagagccg tcgcccgctc ccccacacgg acaccacggc
     8041 agccattctt caggcaagaa ggcactgatc agcctgctgt gccctggcat ggtgctggtg
     8101 tcgctggtgt tctccatcgg agcggtggta gagctcgccg gaaaggcgga gaagatccac
     8161 gaggtaatca agtacgcctg gatggcgggt ctcccgttcc ggatggcaga cggaacgatg
     8221 gcgaggttcg gcgccgattt cggcttcctg ctggacccgc tgagctccgt aatgatcctg
     8281 gtggttaccg gggtcggctt tttgattcat gtttacgcgg tcggctacat ggggcacgaa
     8341 ggcggctact accgcttctt cggctacatg aacctgttcg tgttcttcat gctgatgctg
     8401 gtgctggcga acaactacct gttgctgttc gtcggctggg aaggcgtcgg cctgtgcagc
     8461 tatcttctga tcgggttcta tttccacaag aagtccgccg gggacgcggc caacaaggcg
     8521 ttcatcgtga accgcatcgg ggacgcgggc ttcattctgg gcatgctgct gatgttcgga
     8581 gtgctcggca cggtgcgttt cgtcgacgtg aacgcggcgc ttacgagcgg gaggttcgca
     8641 gcggagacgg ccggattcgg tgtgctgagc ctgatggcgc tgctgctgtt cgtgggggcg
     8701 acgggcaagt cggctcagtt ccccctgtac gtctggctgc cggacgcgat ggaaggcccg
     8761 acgccggttt ccgccctgat ccatgcggcg acgatggtga ccgccggtgt gtacatggtg
     8821 gcgagatcga gcgcgttgtt cagtctgacg cccgagacct ccatggtggt ggcggcgatc
     8881 ggggcgttca ccgcgattct ggcagcgtcg atcgcgctgg tacagaacga catcaagcga
     8941 gtgctcgcgt actcgacggt gagccagctt ggttatatgt tcgtcgcctg cggagtggga
     9001 gcgtattggg tggccgtctt ccacctcttc acgcacgcgt tcttcaaggc gctgctgttc
     9061 ctcggctcgg gatccgtgat tcacgcactt tcgggcgagc aagacatgcg gaagatgggc
     9121 gcgctgaagg acaagattcc agtgacgcac tggacgatgt ttatcgcgtc gctggcgatt
     9181 gccggcattc cgggtttcgc gggcttcttc tcgaaggacg agatcctgtg gcaggcatac
     9241 agctcccctc tcggctcgaa agttattttc gcggttgggt tgatcaccgc cgggatgacg
     9301 gcgttttaca tgtggcggct gatgtacctg acgttctacg gcaagtcgcg ggtggagccg
     9361 gcggcggccc cgcacattca cgaatcgccg cgggtaatga cgattccgct gtcggtgctg
     9421 gctgtcggga gcgtacttgc agggtggatc ggagtgccga agctgtggtc ggttttcccg
     9481 gacgcgttcc ggacctttga acactggctg gccccggcgc tggcctccgc ttccgcgcac
     9541 gttgcagaaa cggcgcatca cgatgccgcg accgaatggc tgctcatgct gctttcggtg
     9601 gcggtggcgc tggcgggcat ctggctggcg cggtacatgt acgacatcaa gcccgagaag
     9661 gccgacgcgc tggcgagctc agcgcccgga ctctataaca cgcttctgaa caagtggtac
     9721 gtcgacgaga tttacggatt tctgtttatt gacggcatgg cgaagggcgg cggcactgtc
     9781 ctcgggacgt tcgacaaccg ggttgtggat ggcggcgtca acggggccgg gtggttgacg
     9841 cgcctcacgt caacggtgtc gatctggtgg gacacctgga tcatcgacgg ttcggtgcgg
     9901 ctgatgtcct ttctggtcaa gctgtcttcc tacccgatgc ggattcttca aaccggccag
     9961 ttccaggcct acgcgctggc cttcgtgctg ggcgtattga tctttttcgg ttactacatg
    10021 atgcgctgat cccatgaacg agcacttgtt gagtatcgtc ctcttcacgc cgctggccgg
    10081 catgatcgtg ctgctgttca taccggcggc gcagaagaac ctgatccgcg tctgggcgaa
    10141 cctggtcgca ttggccgggt tcctggtttc gatcccgctg gtgatgcgct acgatcgcac
    10201 gtcggccggg ttccagatga tcgagaagtt tgactggatc ccggcgctcg gcgccaagta
    10261 ttacgtcgga atcgacggca tcagcctgct gctggtgatg ctgacgacac tggtgggttt
    10321 cctggcgatc ctgtcgtcgt ggagcgcggt cgaagaccgg ctgaaatcgt attacgcgat
    10381 gttcctgctc cagcagacag gaatgatcgg tgttttcctc gccctcgact tcttcctgtt
    10441 ctacgtgttc tgggaagtag tgctggtgcc gatgtacttc atcatcggtg tctggggagg
    10501 gccgcggaag ctttacgcgg cgatcaagtt cttcctgtac accctggcgg gcagcgtatt
    10561 gatgctgctc ggcatcctga cgttgtactt ccagcacttc cggcagtttg gcttctacac
    10621 attcgagatc gccgacctga tgaaactgga cctgccgctc gggctccagc agtgggtgtt
    10681 ctgggcgttc ttcatcgggt tcgccattaa ggtgccgatg ttcccgttcc acacctggct
    10741 tccggacgcg cacgtggagg cgccgacggc cggctccgtg atcctggccg cggtgctgct
    10801 gaagatgggg acttacgggt tcctccggtt ctcgctgcca cttctgccca agg
//
LOCUS       DATIFQ010000507         8054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1705180, whole genome shotgun sequence.
ACCESSION   DATIFQ010000507 DATIFQ010000000
VERSION     DATIFQ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705180"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..916
                     /locus_tag="VLE22_25630"
     CDS             194..916
                     /locus_tag="VLE22_25630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17856.1"
                     /translation="MRGVLGITLAFFFALPALAQFGFYRGTPSVAGRSFGHGFGNVVF
                     PGTGGPSRFSITDPSFASRVSGIVSGYRPYTGAPSGRSYGGGGYVPVAYPVYIGGYGG
                     YPDYPQQPAPNITIINNPPQQTTPQVVINQSFGAEYGKPPTQESTSGEQGVRYYQAPV
                     PEPPARAVSPEQDSKPTVYLIAFKDSTVRAAVGYWIEGDTLHYVTTKGRPNKASLDLV
                     DEELSLQLNRERSVEFELSSAR"
     gene            complement(902..1354)
                     /locus_tag="VLE22_25635"
     CDS             complement(902..1354)
                     /locus_tag="VLE22_25635"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HSB17857.1"
                     /translation="MTRIPGQSSKSAPRLRRMEVAAAVIVRQNKILICQRRHDDSHAL
                     KWEFPGGKLEPGESPAAALQRELEEELCIQARIGPLIDSYDYRYGPRPPIRLFFHRVS
                     EFFGELENQVFEQIRWENLQQLPEYDFVAGDLDFVKRLARGDYRLSRR"
     gene            1415..1780
                     /locus_tag="VLE22_25640"
     CDS             1415..1780
                     /locus_tag="VLE22_25640"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HSB17858.1"
                     /translation="MYSERLLDHFRNPRNVGELPPPAVTVEVSNPVCGDILRLSVRFE
                     DDAAAEVRYKVRGCTASIAAGSALTEWLHGKSRAEMAAVSAALVEEAAGGLRTESKHA
                     AVLCVDAVRALLRTGRPAV"
     gene            complement(1743..3419)
                     /locus_tag="VLE22_25645"
     CDS             complement(1743..3419)
                     /locus_tag="VLE22_25645"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSB17859.1"
                     /translation="MSLSPAPVGPRPATLIVLDPAGKRTRVPLRPLPFGIGRQSDNQL
                     ALRDSRVSRVHARVVLDGGKYVIEDLRSRHGLFVNGVRVSSQVLNPADRIDFGIEDSY
                     RLIFTLEESEVSRLAEQVSGSVPPGTATGNLTRLRSLVEVARALETSISTDDVLAALI
                     DAALAVTSAERGFLLLRNGEQLDVRIARDRAGTTLEASELRVPRGLIMHALRRRRELL
                     SMNFEPGAAEPAMSRTVADLELRSVICVPLVRVQAGASQETAILSGIAETQGVLYMDS
                     RMGRADLSAANRELLQTLALEASTILENARLLEGERARQRMEEELKVARSIQESLLPR
                     RLPDSGWFRAAGRSLPSHQVGGDYFEVASVAPDCWSVVMADVSGKGVSSALLAALLQG
                     SLLAGPRDPDGSVRMMVRINRFLIERTEGEKYATLFYGAVNESGLLHWINAGHCAPLL
                     LRRGERLRALDSNALPVGLLEEAEYAAETVQLQPGDRLVIYTDGVSEAQNADGAFFGD
                     KRVRDVLHRHETADYAAVFRALDDELTAFTSGAVQKDDITLLVVQFGEAP"
     gene            complement(3419..3931)
                     /locus_tag="VLE22_25650"
     CDS             complement(3419..3931)
                     /locus_tag="VLE22_25650"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HSB17860.1"
                     /translation="MALKSIRVSLEERGVRLTRQRRILLDLIDKSGLHLDAERLYTLA
                     KQEDPKINRVTVYRTLKMLKETGLVDELDLMHWSGDQHYYETRMKQEHAHIICLRCGK
                     VEEFFGDALPRLRRQIESHFGFQILIARTEVGGYCSHCQVLRAQEVQAAAHALTPVES
                     SRGEKPTRSN"
     gene            complement(3948..4682)
                     /locus_tag="VLE22_25655"
     CDS             complement(3948..4682)
                     /locus_tag="VLE22_25655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17861.1"
                     /translation="MASIRQINANRKNARRSTGPTSDEGKARSASNALQHGLLSRDIV
                     LPDEDAEEFTTLAASYTAQLDPQGPLEEFCVRQMVAADWKLRRLLRLETGMLAYGVEY
                     IRDVDDDDDRWSGNSSYEETTVLLGKMISGSSRTTRLSLLTRYESSIRQSWYKALKEY
                     ERLRSLRPPRRPEQAPLSGPPRHQETAGEDIGSGSPEMGLASFRDDSQASANPSPSSF
                     ASLAASRRTGLHAPPAPLMLESKLCR"
     gene            4825..5907
                     /locus_tag="VLE22_25660"
     CDS             4825..5907
                     /locus_tag="VLE22_25660"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB17862.1"
                     /translation="MKSRREFLGMMAAGLAGPESRNRQSGMKYRRLGRTGYLVSEIVM
                     GGGSIAPDNHKHVLLALDMGLNYLDTAPAYGPSEEGYAKVIEARGRDKFFLNTKVSLW
                     DENRSKLYREIFDSLPEPEQKKLRSRANEEIERLGAAQTDYFCDYFEGQDQELDAAAL
                     CDVMEKEYGRRIDRGKNYRQIVLDSVDGSLKRLGTDHLDLLMCPHGANTPRQVSEYPE
                     IFEAFETLRKAGKVRHLGFSAHTGAAGILRAAMKTGVYSAAMVAYNIVNHKFMDEALE
                     EAAKSDFGVIAMKAANPVHRIDEHRKPVEPRRVKLIDEAVPGPWKIPQKAYLWNLRNR
                     NLSAVISNMTNAQMVKDNVPLGGNKA"
     gene            complement(5915..>8054)
                     /locus_tag="VLE22_25665"
     CDS             complement(5915..>8054)
                     /locus_tag="VLE22_25665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSB17863.1"
                     /translation="MFSNMRRLCAHFTPKLVAALLACQVCAAGESHVTGAENPKHQRS
                     ATRVSIAGDKWRLNGRPTYPGTGAEGLLMNVRMVNAVFEDRNRPEFDPEANTGEFIAR
                     IPDYVADGIRAFTLGLQGGAPGYEGAVNSAFNPDGSLRQPYLDRVRRVIEACDRSGAI
                     VILSCYYQRQDQVLRDESSVRAGVVNVARWLTSLGRGNVVLEITNEFGHKGFDHTILR
                     TASGQADLIRLAKGTTPGLLVSTSGLGSGRLPDEVARTADFLLIHFNNTRLDEVPARI
                     GDLKKYRKPIVCNEDDRPLAENLLAAELSASAGASWGLMLQDLNQRFPFDFRGAADAP
                     EIYAKLRQLIGGREWSYFPPPESQGGWRKLERPQDVRDIAGMDPEKLASLREWLLASD
                     RRDFAALVIRRGHVVLEVQRNASSVTATGNVKSCAKAICATVVGIAAESSKRGTVPGK
                     MTLDDPAFDFLPWATPLSDARKAKITVKQLLNHTSGIAPESSGARNQGPWEYVLGHTG
                     DPRTAQLAFEPGANLGYSTHAFYHAALVVEQVTGVPYDQFAIEALLKPIGIEKWWFEY
                     FDGGAEYGRHASHALGLPARELARVAYCMLRAGRWNGRQVVPAWFIAETSEPTHSVKG
                     IKDFGRDAESWSHGWELPARLSAPRGTGIPKDARFKPGSGGQLIAFVPSLDLVVVRQT
                     GGSGAWEYEEYLRRACDSVVPFAPGRRTRP"
BASE COUNT         1553 a         2389 c         2685 g         1427 t
ORIGIN      
        1 cacagtgcta ttcttcttca tggccggtct cctatgtgat tgcacctcga gtgcgcaacc
       61 cattgggagc ttaacgcatc ccgccgggag accggccttc tcatcccatc tcggcctggc
      121 gtaccactac ccgccggcag attccaattg cctccgggca aaagtagcgt taagctcaaa
      181 gcagaggttg attatgcgtg gggtgttggg aataactttg gctttctttt tcgccctgcc
      241 ggcgctggcg cagttcggct tctaccgcgg cacgccgagc gttgccggga ggagcttcgg
      301 ccacggcttc ggcaatgtag tatttcccgg aaccggcgga ccgtcccgat tctcgatcac
      361 cgatccgagc tttgcctccc gcgtcagcgg aatcgtcagc ggctaccgcc catacaccgg
      421 cgcgccgtcg ggacggtcat acggaggcgg aggttacgta ccggtagcct atcctgtcta
      481 tatcggcggg tatggcggtt atcccgacta cccgcaacag ccggcgccga acatcacaat
      541 cataaacaac cccccgcagc agaccactcc acaggtggtc atcaatcaga gtttcggcgc
      601 ggagtacggc aagcccccca cgcaggagtc cacgagcggc gaacaagggg ttcgctacta
      661 ccaggcgccg gtgccggagc cgcctgcgcg cgcggtgagc ccggaacaag actccaagcc
      721 gacggtctat ctgatcgcct tcaaggacag cacggtgcgg gccgctgtgg gctactggat
      781 cgaaggggac acgctgcact acgtcacgac gaaggggcgg ccgaacaagg catcgctcga
      841 cctggttgac gaggaattga gcctgcagct caatcgtgag cgaagcgtcg agttcgagct
      901 ctcatcggcg agataggcgg tagtcgccgc gcgccagcct cttcacgaag tctaagtctc
      961 cagcgacgaa gtcgtactcg ggaagctgtt gcaggttctc ccatcgaatc tgctcgaaga
     1021 cctgattctc cagctctccg aaaaactccg agacgcgatg gaagaagagg cgaataggcg
     1081 ggcgaggacc gtaccggtaa tcatagctgt cgatgagcgg gccgatccgc gcctgaatgc
     1141 aaagctcctc ctcgagctcg cgctgtagcg cggccgcggg ggactcaccg ggctccagtt
     1201 tgccgccggg gaactcccac ttcagggcgt gcgagtcgtc atggcggcgc tggcagatga
     1261 ggatcttgtt ctggcggacg atgaccgcgg cggcgacttc catgcggcga agccggggag
     1321 cggatttcga cgactgtccg gggatgcggg tcatccgtgt cccgtcattc taccgcgcgg
     1381 cggcgggggt ggggctacaa tttgggtacc agcgatgtac agtgaacggc tgctggacca
     1441 tttccggaat ccacgtaatg tgggggagct gcctccgccg gcggtaacgg tggaagtgtc
     1501 gaatccggtg tgcggggaca tcctgcggct gtcggtgcga tttgaagacg acgcggcggc
     1561 ggaggtccgc tacaaggtgc gaggctgcac ggcttcgatc gcggcgggat ccgcactgac
     1621 ggagtggctg cacgggaagt cgcgggcgga gatggcggcg gtttcagcgg ccttggtcga
     1681 ggaggcggct ggagggctca ggactgaatc gaagcacgct gccgtgttgt gcgtcgacgc
     1741 ggtcagggcg cttctccgaa ctggacgacc agcagtgtga tgtcgtcctt ctggacggca
     1801 ccgctggtga aggccgtgag ctcgtcgtcc agcgcccgaa agacggccgc gtaatcggcg
     1861 gtttcgtgcc ggtggaggac gtcgcgaacc cgcttatcac cgaagaaagc accgtcggca
     1921 ttttgggcct ccgatacgcc gtcggtgtag atgacgagac ggtcgccggg ctgaagttga
     1981 acggtttcgg cggcgtactc ggcttcttcg agcaggccca ctggcagcgc gtttgaatcg
     2041 agcgcccgga ggcgctctcc gcggcggagc agcaaaggcg cgcagtggcc ggcgttgatc
     2101 caatgcagga gcccgctctc gttcacggcg ccgtagaaca gcgtcgcata cttttcgcct
     2161 tcggtgcgct cgatcaagaa acggttgatg cgcaccatca tcctcacgct tccgtccggg
     2221 tcgcgcggac ccgctagaag gctgccttgg aggagggctg caagcaaggc ggagctgaca
     2281 ccctttccgg acacgtcggc catgacgacc gaccaacagt cgggcgcgac agaagcgacc
     2341 tcgaagtagt cgccgccgac ctgatgcgac ggcaggctgc gtccggcggc gcgaaaccat
     2401 cccgagtccg gcagccggcg gggaaggaga ctctcctgga tggagcgagc gaccttaagc
     2461 tcttcctcca ttcgctggcg ggcgcgctcg ccctccagca ggcgggcgtt ttccaggatg
     2521 gtggacgcct cgagtgccag tgtctggagg agttcgcggt tcgcggccga aaggtcggcg
     2581 cggcccatcc gcgagtccat gtacaagacg ccctgggtct cggcgatgcc ggagagaatc
     2641 gccgtctcct gggaagcacc ggcttgcaca cgcaccaggg gtacgcagat gacgctgcgc
     2701 aattccagat cggccacggt tcgggacatt gccggctcgg cggcgccggg ctcgaagttc
     2761 atcgacagga gctcgcggcg ccggcggagg gcgtgcatga tcagccctcg ggggactcgg
     2821 agctcggacg cctccagcgt cgttccggca cgatcgcgcg cgatgcggac atcgagctgc
     2881 tcgccgttgc ggagcagtaa gaagccgcgc tcagcactgg tgacggcgag ggcagcgtcg
     2941 atgagggcgg cgaggacgtc gtcggtggaa atggaggttt cgagagcacg ggcgacttcg
     3001 acgagcgacc gcagccgggt gaggttgccg gtagccgtcc ccgggggaac cgagccggat
     3061 acttgttcgg cgaggcggga gacctcagac tcttcgagag tgaagatcag ccggtaggag
     3121 tcctcgatgc cgaaatcgat gcgatcggcg ggattgagaa cctggctgga gacgcggacg
     3181 ccgttcacaa acagtccgtg gcggctccga aggtcttcga tgacatactt gccgccgtcc
     3241 agcacaacac gggcgtgaac gcgggagaca cggctgtccc gcaacgcgag ctggttatcg
     3301 gactgccggc cgatgccgaa cggaagcggg cggagtggca cgcgtgtgcg tttgccggcc
     3361 ggatcgagaa cgatgagtgt ggcgggccgc gggcccacgg gcgccggcga caaactcatc
     3421 aattactccg tgtgggcttt tctcctcggg aactctcgac cggggtgagg gcgtgcgcgg
     3481 cagcctgaac ctcctgcgcg cgaagaacct gacaatgcga gcagtaaccg ccaacctccg
     3541 tccgggcgat gaggatctgg aagccaaagt gcgactcgat ctggcgacga aggcgcggca
     3601 gtgcgtcgcc aaagaactcc tcaaccttgc cgcagcgcag gcagatgatg tgggcgtgct
     3661 cctgcttcat ccgggtttcg taatagtgct ggtcgcccga ccaatgcatg aggtcgagct
     3721 catcgaccaa cccggtttcc ttgagcatct ttaaggtgcg gtagacggtg acacggttga
     3781 ttttcggatc ttcctgtttg gcgagggtat agagccgttc ggcatcaagg tggaggcccg
     3841 acttgtcgat caggtccagc aggattcggc gctgccgcgt gagacgcact ccccgttcct
     3901 ccagcgacac ccgaatactt ttcagcgcca tttccgcctc gttgccgtca ccggcacaac
     3961 tttgactcta acatgagcgg cgcgggaggg gcgtgcaatc cagttctccg tgaagccgcc
     4021 aagctcgcaa aggaagaagg agacggattt gcggacgctt gagaatcatc gcgaaacgaa
     4081 gccaatccca tttccggact gccactgccg atgtcttccc cagccgtttc ctgatggcgg
     4141 ggcggaccgg agaggggagc ctgttccggg cggcgcgggg ggcggaggga gcgcaggcgt
     4201 tcgtactcct tcagagcctt ataccaggac tggcggatcg aggattcgta gcgggtaagc
     4261 agcgaaagcc gcgtggtgcg cgagctgccg ctgatcattt tgcccaacag gacggtggtt
     4321 tcctcgtagg aggaatttcc cgaccagcga tcatcgtcgt cgtcaacgtc gcggatatac
     4381 tcgacgccgt aggcgagcat gccggtttcg aggcggagga gccggcggag tttccagtcg
     4441 gcagcgacca tctggcggac gcagaactct tcgaggggac cctgggggtc tagctgggcg
     4501 gtgtaagagg cggccagggt ggtgaactct tcggcatcct catcgggaag gacgatgtcg
     4561 cgggagagca ggccgtgctg gagggcattg gaggcggaac gggccttgcc ctcgtcggac
     4621 gtggggcccg tactgcgacg ggcatttttc cgattggcgt tgatctgacg tatggaggcc
     4681 ataatgtcac ctcatgctag gcacactgac agatttttgc tgtcagcttt cagctatcag
     4741 ctattgagaa tgaagaagtt agggaactga aaagtgtgtg aatggagaac ttggaggaga
     4801 ataagaagac caaggaggtg agggatgaag agccggaggg aatttctggg aatgatggcg
     4861 gccgggctcg cggggccgga gtcgaggaac aggcaatccg ggatgaagta ccggaggctg
     4921 gggcgaactg ggtacctggt gtcggagatc gtgatgggcg ggggctccat cgcgccggac
     4981 aatcacaagc acgtgctgct ggcgctcgac atgggcctga actacctgga caccgcgccc
     5041 gcgtacggac cgagcgagga aggctacgcg aaggtcatcg aggcgcgcgg gcgggataag
     5101 ttcttcctca ataccaaggt cagcctgtgg gacgaaaacc ggagcaagct gtaccgggag
     5161 atcttcgaca gcctgcccga accggagcag aagaaattgc ggagccgggc caacgaggag
     5221 attgagcgct tgggcgctgc tcagaccgat tacttttgcg actacttcga ggggcaggac
     5281 caggaactgg atgccgccgc gttgtgtgac gtcatggaga aggaatacgg ccgccggatc
     5341 gatcgtggga agaactaccg gcagatcgtt ctcgactccg tggacggaag cctgaagcgg
     5401 ctcggcaccg atcacctgga tctgctgatg tgcccgcacg gggccaacac tccgcggcaa
     5461 gtgtccgaat atccggagat cttcgaagcg ttcgagacgc tgcgcaaggc agggaaagtg
     5521 cggcaccttg gtttctcggc gcacacgggc gccgcgggaa tcctgcgggc ggcgatgaag
     5581 acgggtgtgt actcggcggc gatggtggct tataacatcg tgaaccacaa gttcatggac
     5641 gaggcgctag aggaagcggc gaagagcgac ttcggagtga tcgcgatgaa ggcggccaac
     5701 ccggtgcacc ggatcgacga gcatagaaaa ccggtggagc cgcggcgggt gaagctgatc
     5761 gacgaagccg tgccgggacc gtggaagatc ccgcagaagg cgtacctgtg gaacctgcgc
     5821 aaccggaact tgagcgcggt gatctcgaac atgaccaacg cgcagatggt gaaggataac
     5881 gtgccgttgg gcgggaacaa ggcgtagccg gcggttacgg gcgtgtcctg cgccccgggg
     5941 cgaaaggcac caccgagtca cacgccctgc gtaagtactc ttcgtattcc caagctccac
     6001 tgccgccggt ctgccgtacc acgaccaagt ccaagctcgg gacaaacgcg atcagttggc
     6061 caccggaacc cggcttgaaa cgcgcatctt tggggatgcc ggtcccgcgg ggcgccgaga
     6121 gccgggccgg cagttcccag ccgtgggacc aggattctgc gtcccgcccg aagtctttaa
     6181 tgcccttcac cgagtgggtc ggttcactcg tctcggcgat gaaccaggca gggacaacct
     6241 ggcggccgtt ccaccggccg gcccgcagca tgcagtaggc aacacgagcc agttcccgtg
     6301 cgggcagacc taatgcgtgc gaggcgtgac ggccgtactc cgcgccaccg tcgaaatact
     6361 cgaaccacca cttctcgatt ccgatgggct tgagcagcgc ctcgatggcg aattggtcgt
     6421 acggcactcc ggtgacctgt tccacgacca atgccgcatg gtagaaggca tgggtcgaat
     6481 atccaaggtt ggcgccaggc tcgaatgcaa gctgcgcagt tcgaggatcg ccggtgtgcc
     6541 ccagaacgta ctcccacggc ccctggttgc gcgcgccgga cgactcgggt gcgatcccgg
     6601 atgtgtggtt caagagctgt ttcaccgtga tcttggcctt gcgcgcgtcg ctgagcggcg
     6661 tggcccaggg cagaaaatcg aaggccggat cgtcaagagt catcttcccc ggtaccgttc
     6721 cccgcttgct gctctcggca gcgatgccga cgacagtggc acagatcgcc ttggcgcacg
     6781 acttgacgtt accggtcgcc gtcacgctgc tggcgtttct ctggacttcc agaaccacat
     6841 gaccacgccg gatcaccagc gcagcgaaat cgcgccggtc ggaggcgagc agccattcac
     6901 gaagcgaggc caacttttcg gggtccatgc ccgcaatgtc gcggacatcc tggggccgct
     6961 caagcttccg ccagccacct tgtgactcgg gcggtgggaa ataggaccac tcgcggccgc
     7021 cgatgagctg gcgcagcttg gcgtagattt ccggcgcatc cgccgcgcca cggaaatcga
     7081 acggaaaccg ctggttcaga tcttggagca tcaggcccca ggaagcaccc gcagaggcac
     7141 tcaactcggc ggccagaagg ttttccgcca gcggcctgtc atcttcattg catacgatcg
     7201 gcttacgata tttcttcaga tccccgatgc gtgccggaac ctcgtcgaga cgcgtgttat
     7261 tgaagtgaat gagcaggaaa tcagcggtcc gcgccacctc gtcgggcagc cttccactcc
     7321 ccaacccgct ggtcgagacc agcaggcccg gcgttgtgcc cttcgcgaga cgaatcagat
     7381 ctgcctgccc gctggccgtc ctcagaatcg tgtggtcgaa tcccttgtgc ccaaactcgt
     7441 tggtgatttc gagcaccaca ttgcctcgcc caagcgacgt tagccaccgg gcaacgttga
     7501 ccaccccggc ccgcactgaa ctctcgtcac gaagaacctg atcctggcgc tggtaataac
     7561 agctaagaat cacgatggcg ccgctgcgat cgcacgcctc aatcacgcgc cggacacggt
     7621 cgagatacgg ctggcgcagt gagccgtcgg gattgaaggc ggaattgacc gcgccttcgt
     7681 agcctggggc tccgccctgg agacccaacg taaacgcgcg aattccgtct gccacgtagt
     7741 cggggatcct ggcgatgaat tcaccggtgt tcgcttcagg atcgaactcc ggacggtttc
     7801 ggtcctcgaa aacagcgttg accatccgga cattcatcag cagaccctcg gcgcccgtac
     7861 ctggatacgt tgggcggccg ttgaggcgcc acttgtctcc cgcgatcgaa acgcgggtcg
     7921 cactcctctg atgtttgggg ttctccgccc ctgttacgtg tgactcgccg gcggcgcaca
     7981 cctggcaggc aagcaaggct gcgacgagct tcggagtgaa gtgggcgcac aaccggcgca
     8041 tgttagaaaa catt
//
LOCUS       DATIFQ010000508        18135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_368716, whole genome shotgun sequence.
ACCESSION   DATIFQ010000508 DATIFQ010000000
VERSION     DATIFQ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 18135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18135
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368716"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VLE22_25670"
     CDS             complement(<1..482)
                     /locus_tag="VLE22_25670"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HSB17864.1"
                     /translation="MGRDADEAVDAVYRSDWGLIVATLIRLFSDFEVAEEAAQEAFAA
                     AVDQWRTSGVPEFPRAWIIQTARHKAIDRIRRRTRLKEKVESFAESELSQAIEEPNYD
                     PAEIPDDRLRLIFTCCHPSLALEAQVALTLRTICGLETDEIARAFLVPTATMAQRLVR
                     A"
     gene            567..956
                     /locus_tag="VLE22_25675"
     CDS             567..956
                     /locus_tag="VLE22_25675"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSB17865.1"
                     /translation="MLLIYVDEQALNETDRDQCYGESAQFAHQLNASGQYLAAAPLYP
                     TSTATSVRVRDGKRLVTDGPFAETREQLGGFFLIDAKDLDEAIGIAARIPAGRWGTVE
                     VRPVLEIPGLPPDSIVRPVDFAVPPST"
     gene            1093..1503
                     /locus_tag="VLE22_25680"
     CDS             1093..1503
                     /locus_tag="VLE22_25680"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB17866.1"
                     /translation="MQLNPYLTFNGQCEAAFKFYERCLGGKIEAMIPHAGTPVEQHVP
                     PEWRKKILHARLTMGGEVLMGSDAPPDRYEEPKGFSVSLQIKDPADAERIFAALAENG
                     VVRMPLQQTFWAARFGMLVDRFGTPWMINCDQAT"
     gene            complement(1556..1729)
                     /locus_tag="VLE22_25685"
     CDS             complement(1556..1729)
                     /locus_tag="VLE22_25685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17867.1"
                     /translation="MSSRGSSRRLRQLGKPHRSKIYPPFLHTAEEGHILLYIGVTHLE
                     LDVFAFMDDYMKK"
     gene            1772..3157
                     /locus_tag="VLE22_25690"
     CDS             1772..3157
                     /locus_tag="VLE22_25690"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSB17868.1"
                     /translation="MICLFLFAVVLHAQAPVVSGDSLRQLNDAFTRLAGSLAPAVVAV
                     RSNAYEPAAEESEAGAPVALQQNMGSGVIVTLDGDIVTNAHVVAGATRVRVRLSPGGA
                     APGKSVVRPPGRVLPARIVGLDLETDLALIKVDASDLPHLELADSEQVRQGQIVLALG
                     NPFGLEDSVSMGVISAVARQLHPDSRMIYLQTDAPVNPGNSGGPLVDVNGRVVGINTM
                     LFSQSGGSEGVGFAVPSNIVRTVIEQLRRSGAMKRGDIGLEAQTISPAIAAGLGLPRS
                     DGVVVADVTSRGPADNADIRVGDVVLSLNGKPMENARQFHVNLYLRPVASTVTLEILR
                     GGEKLTKTAVVLERLDTPERLATLVNERQHLVSRLGVLGVALDKKLIDLLPSPPRRSY
                     GILVARTAVTPRGPAGLFLPGDVLYKFNNQPLSTMLDLRGFVDKTPPGETVILQVERG
                     GRTRFIEVPLD"
     gene            complement(3269..7081)
                     /locus_tag="VLE22_25695"
     CDS             complement(3269..7081)
                     /locus_tag="VLE22_25695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB17869.1"
                     /translation="MSEVKSRILAILSAVVCVFVSPAGAQTRYLIGSVTMEDGSAPPK
                     RVLIERLCSGRAVQAGVTDLKGRFVARHEMRETFGAGAVVTAFSRTLFINGLSVCVLR
                     ASLPGYESSTIDLSKLSAASDPRLPPMILRPRPPGTDFNLEISRVPRTARKAWGRAVA
                     AGRKQDWPRAELELRAAAQAAPQFADAWNLLGAVCQNGNKTDEAREAFRRALEANPKL
                     LNTYLLLGRLDLQAGDWQAALRTSNALIQADAEHSFPEAHLHRAMACYNLRDLDGAKA
                     SALETIRLDRKREFPRAEFLLGVIMAAQRNFGEAREHLNLYLELDPKATDAAAVRERI
                     ENLGRQEPEETLPALNLVDARLSPAGEVWIPGGMKALAAIANVEKYGGYANFFSDYCR
                     ALIRNTTRYRSRGVPAYEPRLRAYFASIEELARAGERRGDRTVITLSLAGVEQRKETE
                     RILSLLGWKIVTEEGSTRIEIGDHPEDGLRQPIPAALGIDEISLEEALEAGRSFTIEV
                     PSESVRLAGGDAWSTLTQSGPALPGGLAEAFALERRLAKAYVGLSGIDNDAAAAVVDA
                     VGLRAAVEVHAERLSLYAEAFSISNGVAVTPGGSGTESLWAKLAGASPRDPPAFFRAL
                     LQKDRGALAAFYWVLWRADAARQKFFLASPSRAVHFYEWYRESGALQVARDEPASTWQ
                     TLFIRELPVDESGRVRFPGDKRAWGAADSPDENVLLGLAAPQVLAGVAQLERARGAPL
                     DERSASLLVQHYEEWRALLPYFRRLPMLGRAEFESLAAFTESVRNVDTERRNSMLGAW
                     YSLVELIALGHTAGALDTEQSAGAFRRVCTSLQARDLSAQALACLRTITGESGGLDEA
                     VPSRLLRLDDARRSDFNRVLRSQDVPRLTSLASLAGDTQTVVAISGLVYAAWLDPDSL
                     LISEDPLVLRKHRFADPGKDSGSLFLPAALERSSSISGSYLTGGFAGLDEVARELPRV
                     DSADEPAATAPHSAPKVAAKAALADRPPMPTESVFRASGRLVEVYATVTDGRGRYVDN
                     LPQAEFGLLEEGTPQRITAFESNSSAVSCALLLDSTASMYAALPALKNAALKLLDDLR
                     ETDSVAVYRFDRTVTLLQPFTTDKQAAKRAVLRAFPRDETALYDALTQVAREISGRGG
                     KKVIVVFTDGEDNRSMVQAEAAIRRAKVIGAPVYTVAQGRALEYDELLKQLRNVSKAT
                     GGVPFAIREPREIRAVLERVGQDLAHGYLLAFPPPPAPDNNWRQIDVRLQHPRGYKVR
                     AREGYYPE"
     gene            7373..9802
                     /locus_tag="VLE22_25700"
     CDS             7373..9802
                     /locus_tag="VLE22_25700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSB17870.1"
                     /translation="MTIWNDLRFAVRILCKEPGFAAVATLTVALGVGANTAIFSIVNG
                     VLLRPLPYPEPERLVALHEIIPALEATYPLLPVSARHFIEWRERTTSFEALSVIIPSS
                     ANLVGAGEPERLDAACVSPNLFDTLGVKPVLGRGFVAGEDQAGHDRVAILSDSLWRRR
                     FHSGAAIIGKTITLDAQAHTVVGILPPSFRAPAAFPLGPSSSLPRSEVFRPVVFPQDE
                     TKQLMGAFNYGVVGRLKKGTRKERGLAELNVIAAQLEKVSGEKVNLHASVTPLQDLIV
                     GESRRGLLVLLGAVGAVLLVGCVNLANLTLARGERRTHELAIRTALGASRGQVLRHAL
                     SEALLIAFSGGTLGVAVAAAGLGALVRSAPADIPRLAEVSLDGRVLLFALAITMATGL
                     LFGLAPAWRAARTDPQSALKAGGRSAITTTGGVPFRSALVTAEVGLSAILLVTAALLM
                     TSLARLMRSDKGFQAPTVLTTDLVLPEGKYGEQEQRTRFHERLVAGLEATPGVHSAAI
                     STALPLQGETWIDSVFVQGDTRSPWERPMVNMRFVSADYLRTMGIRLVAGRTFSENDR
                     QRQVAIISERLAHTLWPGQDAVGRQFQRNGGQSFEVIGIAGDVRADVAKRPVAMVYRP
                     YWDWPFRRIMVVARAARDPRSIAGGVRAAIRRLDPDLPVAAFRTMQEMLEESVAQRRF
                     QMLLAAVFAATALLLASVGIYGVVSYSVTRRTNEIGLRLALGAESRNLCAMVLRQAMT
                     PVTLGLAAGVVGALAVGRLLSSLLYEVSPSDPVVIGTVVLLLASVALAACYLPARRAT
                     RVDPLAALRYE"
     gene            10020..12287
                     /locus_tag="VLE22_25705"
     CDS             10020..12287
                     /locus_tag="VLE22_25705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB17871.1"
                     /translation="MCQIVVLLFAAPSASSASQVVGRVLDPQGRIVVGATVRLQTGTS
                     NAAVATTDGQGEFRLDPISPGSYRLTAEAPGFQSSAQDVSVIGDIERLDLTLPRLVGQ
                     HQSVVIAAKTVEPTLDLRNAEVFNRTLFTRDDQIMHQLNAGIDAGQHEGGGKSVEVRR
                     FGFNLDHGGTNGGLKVLVDDMQQNQGTQGHGQGYLGALKALSPELIHDVTVINGPFSA
                     EYGDFSGLGVVHIRQRESLPDQFTVRLQGGNFDTGRAFVAYSPDVEHVDAYVAYEGSY
                     TDGPFQNPGRYRRNNFNANYTRSLSTSEKIGVRVLAGSNNFYSSGQIPLDLVNQGLLD
                     RFGFIDPTHGGRVQLGTISMYYSKLRTNGDTFKADGFLARSLLDLYSNFTMYLNDPLH
                     GDAFQQHDSRLQQGVNAQYTHPHHVGAAAAVFVAGTNFHGNQINVGLYPRQGRVPTRV
                     TTRANAHVTNGAGYGQENLSLFRGRLLLGGGVRFDGFRFAVADRVDPHHGGTQSAGRW
                     QGKGSAAFTPSRRLPLTMTANYGRGINSIDARGVVQRPDQPRIATTDFYQLGTSSNFG
                     RFGISTDVFLIDHSNEQVYVPDDGSFEFKGPSRAYGYEAKASVSITRRLSLNGGVTKI
                     GNAYFRGGDHRTYIDSAPHFVANAALTLAGWRGWSGSFRMRAINHYRLDGQNPSILAS
                     GHTVFDLGLSKQVCRGVEFNLSLDNLTNRDYYETQNYFESRVAPMAPVVARIHATPGY
                     PLTVVAGLTFRFRGK"
     gene            complement(12363..13274)
                     /locus_tag="VLE22_25710"
     CDS             complement(12363..13274)
                     /locus_tag="VLE22_25710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17872.1"
                     /translation="MLPGRMGPRTVWRCLLRVASWIVPGRARPEWRARRESRLNDWWL
                     LVERGELTTCAHQEMALDCWRSFADAFWLRFRRAGLQRFLRSPAAVLSGAAAIVLITV
                     ALTHGLAFTMALARRAVEPTFSPAEGIPAAAWGRDMLFVHAFPLAFAFGVGLALVATG
                     SRPFRHRGWRIWAFLAVKAACVMVVVPLVWIEAGAAIRALMPRDSHQAAYAGLFSIVI
                     LIFVFGNALLWSFADQHKRCPVCLERLALPVSVGSWASVFDPATTELVCENGHGSLSV
                     PETDVTGPGRWTVFDSSWQDLFAGNAR"
     gene            13615..13752
                     /locus_tag="VLE22_25715"
     CDS             13615..13752
                     /locus_tag="VLE22_25715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17873.1"
                     /translation="MRSITPNDRGSHFFVTPKAGEKVDKHRLTQVGRAMKELGVQMTP
                     A"
     gene            13888..14379
                     /locus_tag="VLE22_25720"
     CDS             13888..14379
                     /locus_tag="VLE22_25720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17874.1"
                     /translation="MNRKFAVPAAAKGTVFRRCSRTDLDWIFTVQAERVVAQDNTVAI
                     GDRRWQTDKTRFPHTLAGCTVTIHEHLDGTVSMRYGPHVVDDYDSFPLQRKNFHEGCV
                     VKPDRSRVNRTGHLDMLSTGHSPPLGGAPLRLWCTHSDAHRLSDPSDQPAERSDLQSD
                     HPG"
     gene            14498..16807
                     /locus_tag="VLE22_25725"
     CDS             14498..16807
                     /locus_tag="VLE22_25725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17875.1"
                     /translation="MGANEARGRIAPPAMTPEEFRSLGHRLADQIARLLGESCAGIGK
                     MLPAVVMCLFLGQAAHAVQITSDATPWKLDIGDDGKQLKLLVHRREIGQVCELWCYEG
                     GPFRYGKATKRDDGSVVFVHNSGRMRATTTFTPAGQDRVLMDIVVEGPIEELHRVSLM
                     GPCMQFWHSEAFRRQETLVEFASRCFLYTMRGPVGMMDTARGQMKGYKPDAPENNPPW
                     TQWYVPFGRAHPGDIWAFGASGDRPIHDLVGVSSQDRKWLAAIGCRHTTTLGQGWHDC
                     IHHVPDMQKYLDAGASRIVHRSVLYVMPNDTGKLLESFRQDFEAKPAAEGISVSVANH
                     GRLAVSTRSPTATLLDLSLVVDNAGRRHRAVWKASSWGGHVSDCGTARLWAYAHDGAV
                     ELWASAPSSMPPARVDAALGGKGWSLIHAPKDVPAIVQQSPDGAWTAAMVWEQREAGN
                     PGTGVAAPGTLSSAAASVRGRLLIFKGSVAALQERWVETQQEWRHSMPYRMPPREQKE
                     KVQAGGVRFAPNAEPDMTYGLTIVPPWKDGGTLHSNFPEHLEHGDVGYGILRHHNKRP
                     YPWRIAADGRSADYEVESPELPGVIVRASAVAKGDGAHLTLRITNGGNRRLERVKPLL
                     CFWYAKLTGFPTRNSDNFEHTYVVVDNKPIPLAGIATANPAATAKVAYVHGCSQHDCD
                     KFAQSRGGLIDRDIDHALIVVTARDGKRKVVITFAPGKSILSNKFIPCAHADPYYGTI
                     EPGASAEATGEVYFTDKPIAEIVRAFPRR"
     gene            complement(16983..>18135)
                     /locus_tag="VLE22_25730"
     CDS             complement(16983..>18135)
                     /locus_tag="VLE22_25730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSB17876.1"
                     /translation="AFSWRRLLRVLSPYGGLTVVYFITRWIAMRGTTPFAQAGNYAPT
                     LDLNRALSHLVLYAGDFLNISLANPWIHGTGSFATLFGLPSPHTWERTFLAGGALLFL
                     VTVVPALVRDARVWSGLCLTGAFIAPTLLVGLAQTYYLLVPLAGVAMALAVSLSAWSQ
                     GPRRVLTVLWTAVVLVCVVNCWVMRRNVEGLFWYSAGNAARQAYQSMIEPEKDRPIRS
                     LTLMVADPSIVWYWKWVLDPFSTPGLVQALANKPHLEFRVQEFDLTKARLLVGEVSPV
                     YFLRDGTFAKFADLVIKAGGPLQTRAGQRFNVQPDGTSAIWLQTENVPRSSVVILGDR
                     RLPSMVAGATAVSCTVPRELYAKPGKYPIYVEDALTGRRSNALQFTVAP"
BASE COUNT         3496 a         5661 c         5662 g         3316 t
ORIGIN      
        1 gctctcacga gccgctgcgc cattgttgcc gtcgggacga gaaacgcgcg agcgatctcg
       61 tccgtctcca ggccgcagat cgtgcggagc gtcagcgcga cctgcgcctc gagcgcaagc
      121 gacgggtggc aacaggtgaa tatcaggcga aggcgatcat cgggaatttc ggccgggtcg
      181 tagttgggct cctcgatggc ttggctcaac tccgactcgg cgaatgattc gactttttct
      241 ttgagcctcg ttcgacgccg gatacggtcg atggccttat ggcgagcggt ctggataatc
      301 caggcgcgag ggaactctgg cacgccggaa gtgcgccact gatcgacggc ggccgcaaag
      361 gcttcctgcg ccgcttcttc ggccacctcg aaatccgaaa agagccgaat cagcgtggcg
      421 acaatgaggc cccagtcgga gcgatagacg gcgtctacag cctcgtcggc atcccgcccc
      481 atatacccaa ttgtaaacaa ttcaccgagc tgtcgatccg ggcgcgggcc gttcgactga
      541 ctcatggagg cagaggcgtg aaatacatgc tgctgattta cgtcgacgag caggccttaa
      601 acgagactga ccgggatcag tgttacgggg agtccgcgca gtttgcgcac cagctcaacg
      661 caagcggaca gtatctggcg gctgcgccgc tataccccac ctcgacggcg accagcgtcc
      721 gtgttcgtga cggcaaacgg ctcgttaccg acggtccgtt cgcggagacg cgcgaacaac
      781 tcggcggctt cttcctgatc gacgccaagg acctcgacga ggcgatcggc atcgccgccc
      841 gcatcccggc agggcgctgg ggcacggtcg aagtccggcc ggtgctggag atccccggcc
      901 tgccgccgga ttcgatcgtt cgacctgtcg atttcgctgt tcctccttcg acctaatgga
      961 agagcgcggt tgccaacggt cgaaacggtt tcgaaagtac aaacccatag ctctgggccg
     1021 ggccgcctgc gcgcgcttat ccctttacgg agtcagcagc gaacctcttg gaccaaatga
     1081 aaggagtgca ccatgcaact gaacccttac cttacgttca acggccagtg cgaggcagcg
     1141 ttcaaattct atgagcggtg tcttggcggc aaaatcgaag ccatgattcc ccacgctggt
     1201 actccggtgg aacaacatgt gcccccggag tggcgcaaga agattcttca tgccagactc
     1261 acgatgggcg gggaggtctt aatgggctct gacgctccgc ctgaccgcta tgaagaacca
     1321 aaaggattct ccgtgtcgct ccagatcaag gacccggccg atgcggaacg catatttgcc
     1381 gcactggcag aaaacggagt ggtgcggatg ccgctccagc agaccttctg ggccgcccga
     1441 ttcggcatgc tcgtcgatcg attcggcaca ccgtggatga ttaactgcga ccaggctacc
     1501 tgaccgcgct ccgatgctct gagcgcggct gatgcacagc tcgcagcata caggctcact
     1561 tcttcatgta atcgtccatg aaggcgaaca cgtccagctc caaatgggtc acgccgatat
     1621 acagcaagat gtgcccctcc tccgccgtgt gaaggaatgg cgggtagatc ttcgagcggt
     1681 gtggcttgcc gagttgccgc aggcgccggc tggagccccg gctggacaat gccctccgac
     1741 gcggttatcg tgggctgata tgaggggccg cttgatctgt ctgttcctgt ttgctgtagt
     1801 tctgcacgcc caggcgccgg tcgtttccgg cgattctctc cgccaactca acgatgcgtt
     1861 tacgcgcttg gccggctcgc tcgcgccggc ggtggtcgct gtccgcagca acgcctatga
     1921 gcccgcggct gaagagtccg aggcgggcgc gcccgttgcg ctccagcaga acatgggttc
     1981 cggcgtgatt gttaccctgg acggcgatat tgtcacgaat gcgcacgtcg tcgccggagc
     2041 cacgcgcgtc agggttcgac tttcccccgg cggcgcggcg cccgggaaga gcgtcgtccg
     2101 tccacccggc cgggtattgc ccgcccggat cgtcggtctc gatctggaaa ctgacctcgc
     2161 cctgatcaaa gtagacgcct cggacctccc gcacctcgaa ctggcggatt ccgagcaggt
     2221 gcgccagggc cagatcgtgc ttgccctcgg caatccgttc ggcctcgagg attcggtcag
     2281 catgggcgtc atcagcgctg tcgcgaggca gctccacccg gacagccgca tgatctacct
     2341 ccagaccgat gcgcccgtca atccaggaaa tagcggcggc ccgctggttg acgtcaacgg
     2401 acgtgtcgtc ggcatcaaca ccatgctgtt cagccagtcg gggggcagcg aaggcgtcgg
     2461 cttcgccgtt ccgtccaaca ttgtccgcac cgtaatagag cagttgcgcc gttccggtgc
     2521 catgaagcgc ggcgacatcg gcctcgaagc gcagaccatt tcaccggcca tcgcggccgg
     2581 cctcggcctc ccgcgcagcg atggcgtggt ggtcgcggac gtcaccagtc gtggacctgc
     2641 cgacaatgcc gacatccgcg tcggcgacgt cgtgctctcc ctcaacggca agccgatgga
     2701 gaacgcgcgc cagttccacg tcaacctcta cctgcggccc gtcgcttcca cggtgacgct
     2761 cgaaatcctc cggggcggtg agaagctgac gaagacggca gttgttctcg aacggctcga
     2821 cacgcccgaa cgcctcgcta cgcttgtcaa cgagcgccag cacctcgtct cgcgcctcgg
     2881 cgtcctagga gtcgccctgg ataagaagct gattgacctc ctgccgtcac ccccgcgccg
     2941 gagctacggc atcctcgtgg cccggaccgc agtgaccccc cgcggtccgg cagggctatt
     3001 cctgccaggc gacgtcctgt acaagttcaa caatcagccg ctgtcaacga tgctcgacct
     3061 gcgcggcttt gtcgataaga ccccgcctgg cgagacagta atcctccagg tggagcgcgg
     3121 cggcaggacc cgctttatcg aggtccccct cgactagtgc cgtcccaact cctcccggct
     3181 aagggcccgc gccacgcagc gagccgctgc gacccctgcg atcagccgtt cgagagtcag
     3241 caagttggaa cggcactagc cagctagcct actccgggta gtaaccctct cgtgcgcgca
     3301 ccttgtagcc cctggggtgt tgcagccgga cgtcgatctg gcgccagtta ttgtccgggg
     3361 ccggcggagg cgggaaggcc agtagataac cgtgcgccag atcctgacct acgcgttcga
     3421 ggacagcacg gatctcccgc ggttcacgga tcgcaaacgg tactcctccg gtcgccttcg
     3481 aaacgttcct gagttgcttc agtagctcgt catactcgag cgcccgtccc tgagccacgg
     3541 tgtacaccgg cgcgccgatg actttggcgc gccggatggc cgcttcagcc tgcaccatgc
     3601 tccggttgtc ttcgccgtcg gtgaagacga caatcacttt ctttccgccg cggccggaga
     3661 tttcacgggc aacttgcgtc agggcatcgt acagcgcggt ctcatcgcgg gggaatgcgc
     3721 gcaaaaccgc tcgtttcgcc gcttgtttgt ctgtggtaaa cggttgtagc agcgtgaccg
     3781 tccggtcgaa acgatagact gccaccgagt cggtctcgcg gaggtcgtcc agcagcttta
     3841 gggcggcgtt cttcagtgcg ggcaaagcgg catacatgct cgccgttgag tccagcaaaa
     3901 gagcacagga gacggccgag gagttgcttt caaacgccgt gatccgctgg ggcgttccct
     3961 cctccagcag cccaaactcc gcttgcggaa ggttgtcgac ataacggccc cttccgtccg
     4021 tcaccgtcgc gtatacctcg accagccgtc cactggcgcg gaagaccgat tcggtcggca
     4081 tcggcggacg gtccgcgaga gcggctttgg cggcgacctt cggagccgaa tggggggcag
     4141 tagccgccgg ctcatcagcg ctgtccacgc gcggaagttc ccgcgctacc tcgtcaagcc
     4201 cggcaaaacc tccggtcaaa tagctgccgg agatactgct ggaccgctcg agcgcggcgg
     4261 gaagaaatag ggaaccggag tcttttcccg ggtcggcgaa gcggtgcttg cgaagcacga
     4321 gcggatcctc ggaaatcagc aacgagtccg gatccaacca cgccgcatag accagcccgc
     4381 ttatagcgac cacagtctgg gtgtcaccgg ccaacgaagc cagggacgtc aggcgcggca
     4441 cgtcctgcga tcgtagcact cgattaaagt ctgatctccg tgcgtcgtcc aggcgaagca
     4501 gacggctggg aaccgcttca tccaggccgc cggattcacc tgttatcgtg cgcaggcaag
     4561 ccagggcctg ggccgagaga tcacgcgcct gcagtgacgt gcaaacgcgc cggaacgcgc
     4621 cggcgctctg ttctgtatcg agggcgccag cggtgtggcc gagcgcgatc aactcgacca
     4681 gcgaatacca tgcgcccagc atggaattcc tccgctccgt gtcgacgttt cgaacagact
     4741 ccgtgaacgc cgccagactc tcgaattcgg cgcgccccag catcggtagg cgtcggaagt
     4801 acgggagcaa agcgcgccat tcctcgtagt gctgcacgag caggcttgcc gagcgttcgt
     4861 cgagaggggc tcctcgggcc cgctcgagtt gcgcgacccc tgcgagaacc tgcggagcgg
     4921 ccagacccag caggacattc tcgtccggtg aatcggcagc gccccaggca cgtttgtctc
     4981 ccgggaaccg cactctccca ctctcatcga ccggcaactc gcggataaag agggtctgcc
     5041 atgtcgatgc aggctcatcc cgggccacct ggagcgcgcc ggattcccgg taccactcat
     5101 agaagtggac cgcgcgtgac gggctcgcca gaaagaattt ttgacgggcc gcatcggcgc
     5161 gccacagcac ccaatagaaa gccgccagcg caccacggtc tttctgaagc aacgcgcgga
     5221 agaaggccgg cggatcgcgc gggcttgcgc ccgcgagctt cgcccataag ctttctgtac
     5281 cggagcctcc aggcgttacc gccactccgt tggagatcga aaacgcctct gcatatagcg
     5341 acaagcgctc cgcatgaacc tccactgccg ctcgcagccc cacggcgtcc accaccgccg
     5401 ccgcggcatc attgtcgatc ccgctcagcc caacgtaggc tttcgcgaga cgtcgctcaa
     5461 gcgcgaaggc ttccgccagg ccgcccggca gagccggacc gctctgtgtc agagtgctcc
     5521 acgcgtcgcc ccccgccagc cgaacgcttt ccgacggtac ttcgatcgtg aagctgcggc
     5581 ccgcttcgag ggcctcctcc aacgagattt catcgatgcc gagagcggcg ggaatcggct
     5641 ggcgcagccc gtcttccgga tggtcgccga tctcgatcct ggtggaacct tcctctgtca
     5701 cgatcttcca ccccaagagg gacagaatgc gttcagtctc ctttctttgc tctacgccgg
     5761 cgagcgacag ggtgattacg gtgcggtcgc cgcgccgctc cccggcgcgc gcaagttctt
     5821 cgatcgaagc aaagtatgcc ctcaggcgag gttcgtatgc gggcacccca cggctgcggt
     5881 agcgcgttgt attccgaatg agcgcccggc agtaatccga gaagaagttc gcgtagcccc
     5941 catacttctc gacgttcgcg atggcggcca gcgctttcat cccacccgga atccacacct
     6001 cgcccgcagg cgagagccgt gcatcaacca gattcagagc cggaagagtt tcctccggct
     6061 cctgcctgcc gaggttctcg atacgttcac ggaccgccgc tgcgtccgta gccttgggat
     6121 ccaactccag atacaggttc aggtgctccc tggcctcacc gaagttgcgc tgcgctgcca
     6181 taatcacacc aagcagaaac tcggcgcgcg gaaactcgcg cttccggtcc aagcggatcg
     6241 tttccaatgc gcttgccttc gccccgtcga ggtcgcgcaa gttgtaacag gccatggcgc
     6301 ggtgtaagtg ggcctcgggg aaggagtgtt cggcatccgc ctgaatgaga gcattactcg
     6361 tccgaagcgc cgcctgccag tccccggcct gcaaatccag acgccccagc aggagatatg
     6421 tgttcaacag tttcggattg gcttccagag cccgccggaa cgcttcccgc gcttcgtccg
     6481 tcttgttccc gttctggcac accgccccga gcaggttcca tgcatctgca aactggggcg
     6541 ccgcctgggc agccgcccgg agctcgagct ccgcacgcgg ccaatcctgt ttccgtcccg
     6601 ccgcgaccgc gcgtccccac gctttccgcg cggtacgcgg tacgcgcgaa atttcaagat
     6661 tgaaatccgt gcccggtggg cgcggccgaa ggatcatcgg cggcaggcgc ggatcggatg
     6721 cggcactcag cttggagaga tcgatcgtgc tcgactcata ccctggaagc gaggcccgaa
     6781 gcacgcagac ggacaacccg ttgatgaaca gcgtgcgtga aaacgcagtc accaccgcgc
     6841 cagcgccaaa cgtctccctc atttcgtgtc gcgcgacaaa cctccccttc aaatcggtca
     6901 cacccgcctg cactgcgcgt ccgctgcaga gacgttcgat aaggacccgc tttggaggtg
     6961 ccgagccatc ctccatggtc acgctgccga tcagataacg tgtctgcgcg cctgccggtg
     7021 acacgaagac gcagacgacc gcagacagta tggcgagaat cctgcttttc acttcgctca
     7081 ttttttcatc agggactact ggcagtgtat tccaattcaa cataccgcca ttggcggttt
     7141 catgtcgcgg gggatcagat tgccgcacca cgacaatctg aagagagaaa ggcataacgg
     7201 gcggcttcga tcgcatgagc tgtgcttttg cgcacgcaat ctgagcatcc gccgcaacca
     7261 tcgctacaag cagcataggc cgttaaggga agtcgtgacc gcgagttgaa ccggcccgat
     7321 tatccgagaa cccaagccct cggaagagcg tattcaggtt aagaaggcct ttatgactat
     7381 ttggaatgac ctgcgctttg cggtacggat tctctgcaag gaaccaggct ttgccgcagt
     7441 ggcaaccctg acggtagcgc tcggagtggg tgccaacacc gcgatttttt cgatcgtcaa
     7501 cggcgtactg ctgcggccac tgccgtaccc cgaacccgag cggctggtgg cgctgcacga
     7561 gattatcccg gcgctggagg caacataccc tttgctgccg gtgagcgcgc gtcacttcat
     7621 cgaatggcga gagaggacaa cgtcgttcga agccctttcg gtgattattc ccagtagcgc
     7681 gaacctcgtc ggggctgggg aacccgagcg gttggatgcg gcgtgcgtgt cgccgaacct
     7741 gttcgacaca ctgggagtga aaccggtgct cgggcgcggt tttgtggcgg gcgaagacca
     7801 agccgggcac gaccgcgtcg ccatcctcag cgactcgctg tggaggcgga ggtttcattc
     7861 cggcgcggcc atcatcggca agacgatcac gctcgacgcg caggcgcata ccgtggtggg
     7921 aatcttaccg ccgtctttcc gtgcgccggc cgccttcccg cttggaccgt cttcctcgct
     7981 accgcggtct gaggtcttcc ggccggtcgt gtttccgcag gatgagacaa agcaattgat
     8041 gggagcgttc aactacgggg tggtcgggcg gttgaagaag ggaaccagaa aagagcgtgg
     8101 gctggccgag ttgaacgtga tcgctgcgca actggagaag gtatccggag agaaggtgaa
     8161 cctacatgcc tcggtgacac cgttgcagga cttgatcgtt ggggagtccc ggcgcggcct
     8221 gctggtgttg ctcggagcgg tcggggcagt gctgctggtc ggctgtgtga acctggcgaa
     8281 ccttacgctg gcgcgaggcg agcgccggac acacgaattg gcgatccgga cggcgctggg
     8341 cgccagtcgt ggccaggtgc tgcggcacgc gctcagcgag gcgctgctga tcgcattctc
     8401 aggcggtacg cttggcgtgg cggtagcggc tgcggggttg ggggctctgg tccgcagcgc
     8461 gccggcggat attccgcggc tagccgaagt gagtcttgac gggcgtgtgc ttctgttcgc
     8521 gctcgcgatc actatggcca ccggtctgct gttcgggctg gcgccggcgt ggcgtgcggc
     8581 gcgcaccgat ccgcagagcg cccttaaagc cggtgggcgg agtgccatca ctaccaccgg
     8641 tggtgttccg ttccgcagcg cgctcgtgac ggcggaggtg ggcttgagcg ccatactgct
     8701 cgtgacggcc gcgctcttga tgaccagcct cgcacggctg atgcgctcgg acaagggctt
     8761 ccaggcgccg acggtgctca ccaccgatct tgtacttccg gaggggaaat acggcgagca
     8821 ggaacagcgg acccgattcc acgagcggct ggtagccggg cttgaagcca cgccgggtgt
     8881 gcattcggct gcgatctcga ccgccttacc actgcagggt gagacatgga tcgacagtgt
     8941 gtttgtgcag ggtgacacgc ggtcgccctg ggagcgcccg atggtcaaca tgcgctttgt
     9001 cagcgcggac tacctgcgta cgatgggcat tcgacttgtt gccgggcgga cgttcagcga
     9061 gaacgaccgc cagcgccagg tcgccatcat ctcggagcga ctggcgcaca cgttgtggcc
     9121 cggccaggac gcggtagggc gtcagttcca gcgcaacggc gggcagagct tcgaagtaat
     9181 cgggattgcg ggggacgtcc gggctgacgt agctaaacgg ccggtggcaa tggtttatcg
     9241 cccttattgg gactggccgt tccgccggat catggtggtg gcgcgagccg cgcgcgatcc
     9301 gcgctcgatt gcaggcgggg tgcgggcggc gatccgccgc ttggatccgg acctcccagt
     9361 tgcggcgttt cgcacgatgc aggaaatgct ggaagaatcg gtagcgcaac gccggttcca
     9421 gatgctgctc gccgccgtgt tcgccgcgac ggcactgttg ctggcgagtg tgggtatcta
     9481 tggcgtggtc tcgtactcgg tgacacgacg aaccaatgag attggtctgc gcctggcgct
     9541 tggggcggag tcgcgtaatc tctgcgcaat ggtcctccgg caagcgatga cgccagtaac
     9601 gctgggcttg gcggcgggcg tggtgggagc gctggcggta gggcgcctat tgagcagtct
     9661 gctgtacgag gtcagcccga gcgatccggt cgtaatcggc acggtggtac tgcttctggc
     9721 ctcggtggcg ctggcagcct gttacctgcc cgcacggcga gccacacggg tggacccgtt
     9781 agctgcgctg cggtacgagt aaatgtccgg ttgcgccggc atcgtcgcgg tccgaaaccc
     9841 ttgaccgaag cccggaaatc gtcgttcgag acaactagcc tacatccaac ttcgctcgcg
     9901 agaacgtagt tctcatgagg ttcggcacga ccaggatgag tattacgtat tgtataatcg
     9961 tgctacacag agcgatgcaa tttcagtctt caattcaccc cggaagaata tcgacactag
    10021 tgtgccaaat cgtggtgctg ttgttcgcgg cgccgtccgc tagtagcgcc tctcaggtcg
    10081 ttggccgggt actcgaccca caagggcgca tcgtggtggg agcgactgtc cggctgcaaa
    10141 ctgggacgtc caatgccgcc gttgcaacca ccgatggaca gggcgaattc cgtctcgacc
    10201 ccatcagtcc cggctcctat cggctcacag cggaggcccc gggatttcag agcagtgctc
    10261 aagacgtcag tgtcattggc gatatcgaga ggctggacct tacgctgccc cgccttgtgg
    10321 ggcagcatca gagcgttgtg atagcggcga agacagtcga gccaactttg gatcttcgca
    10381 acgcggaggt cttcaatcga acgctcttca ctcgcgacga tcagatcatg caccaactga
    10441 acgcaggaat cgacgcaggt cagcatgaag gcggcgggaa gtccgtcgag gtccggcgat
    10501 ttgggttcaa tctcgaccac ggtggcacga acggcggtct caaggtgctc gtggatgata
    10561 tgcagcagaa ccagggaacg caagggcacg ggcagggcta ccttggggcg ctcaaagctt
    10621 tgagcccgga actgattcac gacgtcacgg tgataaacgg cccgttcagc gcggaatacg
    10681 gagacttcag cgggcttggt gtagtccaca tccgccagcg ggaatcgctg ccggatcagt
    10741 tcacggtgcg gcttcaaggt gggaactttg acacgggccg ggcctttgtg gcctatagtc
    10801 ccgatgtcga acacgttgac gcttacgtgg cctacgaagg ttcatacacg gacgggccgt
    10861 ttcagaatcc aggccgttat cgccggaaca atttcaacgc caactacacc agatcgctca
    10921 gcacaagtga gaagatcggc gtccgcgttc tcgccggctc aaacaacttc tattccagcg
    10981 ggcagatccc tctcgatctc gtaaaccaag gcttgctcga tcgattcgga ttcatcgacc
    11041 caacccacgg gggccgggtg caacttggta ccatatcgat gtactacagc aagctgagga
    11101 ccaatggcga tacgttcaag gccgacgggt ttctggcacg gtcgcttctc gacctgtact
    11161 cgaacttcac gatgtacttg aacgatcctc tacacggcga tgctttccag cagcacgatt
    11221 cgcgcctgca gcagggtgtg aacgctcagt acacgcaccc gcaccatgtc ggtgcggcag
    11281 cggccgtctt cgtagcggga accaacttcc acgggaatca aatcaacgtc gggctttatc
    11341 ctcgtcaggg gcgggtccct acgcgtgtga cgaccagggc caatgcccac gtgacgaacg
    11401 gtgcgggata tggacaggag aacttgagcc ttttccgtgg acggctcctg ctgggcggcg
    11461 gcgttcgctt tgatgggttc cggttcgcgg ttgccgacag ggtcgacccg catcacggcg
    11521 gcacgcagtc ggcgggacgc tggcaaggaa aaggcagcgc agcgttcact ccatcacgcc
    11581 gcctgccgct cacgatgacg gccaactacg ggcgcggcat taacagcatt gacgcccgcg
    11641 gcgtcgtcca gcggcccgac caaccgcgaa tcgcgaccac ggatttctat cagcttggaa
    11701 cctcatcgaa ctttggacga ttcggaatca gcacggacgt gtttctcatc gaccactcca
    11761 acgaacaggt ctacgtgccg gacgacggca gcttcgagtt taagggaccc agccgggcct
    11821 acggctacga agccaaggca tcggtgtcca tcacgcgacg gctctccttg aacggtggtg
    11881 tcaccaagat tggaaatgcg tacttccgag gcggtgatca ccggacttac atagacagcg
    11941 cccctcattt cgtggcaaat gccgccctca cactcgcagg atggcgcggt tggagcggct
    12001 catttcgcat gagggcgatc aaccactatc ggcttgacgg ccagaatcct tccattcttg
    12061 cttcgggaca caccgtcttt gatttgggcc tctccaagca ggtgtgccgg ggagttgagt
    12121 tcaatctgag cttagataat ctgacgaacc gtgactacta cgaaacgcaa aactacttcg
    12181 agtcacgggt tgcaccaatg gcccccgtcg tggcaagaat tcacgcaacg ccgggatacc
    12241 cgctcacggt ggtggccggc ctgaccttca ggttccgggg gaaatgactc acgggtcgcc
    12301 ctccggctag gcagaatcgg agttccctct ccgtatccca aattgacggg attcccggga
    12361 ctctatctgg cgttgcccgc gaagaggtcc tgccacgagg agtcgaatac ggtccagcgg
    12421 cccggtccag tgacatcggt ttccgggacc gaaagcgagc cgtgcccgtt ctcgcacacc
    12481 agttccgtgg tagccgggtc gaagacactg gcccacgaac caaccgagac cgggagagcg
    12541 agcctttcaa gacaaacggg acaccgcttg tgctggtccg caaagctcca cagcaacgcg
    12601 ttcccgaaga caaagatgag gatcacaatg gaaaacaacc cggcataagc agcctggtgg
    12661 ctgtcacgtg gcatgagggc gcgaatcgca gcgccggcct cgatccacac aagtggcacg
    12721 accaccatca cgcaagcggc cttcaccgcc agaaatgccc aaatcctcca gcctcggtgc
    12781 cggaaaggtc ggcttcccgt tgctacgagt gcgagtccga cgccgaaagc gaaggccagc
    12841 gggaaggcgt gcacaaacaa catatcccgc ccccaggccg cagccggaat gccttccgca
    12901 ggcgagaacg tgggctccac cgcccgacgg gccagcgcca tggtgaacgc caggccgtgt
    12961 gtgagcgcca ccgtgatcaa gacgattgcc gctgcaccgg agagcactgc agccgggctg
    13021 cgcagaaatc gctggagccc ggccctccgg aagcgcagcc agaacgcgtc cgcgaagctg
    13081 cgccagcaat ccagtgccat ctcctgatgg gcgcaggtgg ttaactcccc gcgctccacc
    13141 aaaagccacc agtcgttgag gcgggactcg cggcgagccc gccactccgg ccgggcgcgt
    13201 ccgggcacga tccaactcgc gactcgcagc aggcagcgcc agaccgttcg cggacccatc
    13261 cttccgggca gcatagctac ctccgtcgcc gtcaaacagg tagacgcctt gcgtgccgcg
    13321 aatgttccct tgactggaga tcgcgtcatc agaggagcga gcacctgatg cgcaggacag
    13381 tcaagtcgct cggtggtggg cgccggggag cctgacccgc ttgacgggtt aaaacggagg
    13441 cagtagtctg aaatctgccc cgtgaccagc accggaacta tctaacttac aacacacgtt
    13501 cacacggggc agtttatctc ctacccatgc tgctgcacat cgacggcagc aagcaccgct
    13561 ggtttcagga cgagcgctgg tacgacttga tagtgatctt ggacgacgcc agcagtgaga
    13621 tctattacgc ccaacgaccg ggggagtcat ttcttcgtga cgccgaaggc cggcgagaag
    13681 gtggacaagc accgcttgac gcaggtggga cgggcgatga aggagttggg ggtgcagatg
    13741 actccggcct aatcgccgca ggcgcggggc cgcagcgagc ggaactttgg cacctggcag
    13801 gggcggctgc cgcaagagct gcggctggcg gggatcggga cgccggagca agccaaccag
    13861 tttctgcgtg agcgctatat tgccgggttg aaccggaagt ttgcggtgcc ggcggccgct
    13921 aagggcacgg tgtttcgccg gtgttcgcgc accgacctgg actggatctt cacggtgcag
    13981 gccgagcgtg tcgtggccca ggacaacacc gtggccatcg gagatcgtcg ttggcaaacg
    14041 gacaagacgc gcttcccgca cacgctggct ggttgtacgg tgacgatcca cgagcacctg
    14101 gacggcacgg tgtcaatgcg ctacggtccg cacgtggtgg acgactacga ttccttccct
    14161 ctccagagga aaaacttcca cgagggctgc gtagtgaaac cggacagatc acgtgtgaat
    14221 agaaccggac atcttgacat gttatcgaca gggcattcac cgccgcttgg cggcgctcca
    14281 ttgaggctct ggtgtactca ttctgatgca catcgactga gtgacccatc tgatcagccg
    14341 gcagagcgga gtgatctcca gagcgatcat cccggataac acgcgggaag gcggcgccct
    14401 gacgtccttc ttatctggcc ttccagatga ggagctgcgt tcgctgcgtg actggggcac
    14461 cgacgaccga agaagcgcgg acgtttagaa ttgacctatg ggagcgaacg aagcgcgagg
    14521 gcgtattgcg ccgcctgcaa tgacccccga ggaatttcgc agcctgggcc accggcttgc
    14581 cgatcagatc gcgcggcttc tcggtgaatc gtgcgcgggg atcggcaaga tgctgccagc
    14641 cgtggtgatg tgcctctttc tcggccaggc ggcccacgcc gtccagataa ccagcgacgc
    14701 cacaccgtgg aaattggata ttggcgacga tggaaagcaa ctcaagcttc ttgtgcaccg
    14761 tcgtgaaata ggccaagtct gcgagttgtg gtgctacgag ggtggtccgt tccgttacgg
    14821 gaaagcaacg aaacgcgatg acggcagcgt cgtctttgtt cacaactctg ggcggatgag
    14881 ggctacgact accttcacgc cggcgggaca agaccgcgtc ctgatggaca tcgtggttga
    14941 aggcccgatc gaggaactgc acagagtcag tctgatggga ccctgcatgc agttctggca
    15001 ctcggaggcg ttcaggcgcc aggagacact tgtcgagttc gcctcacgct gtttccttta
    15061 cacgatgcgc ggaccggtgg ggatgatgga tacggctcgc ggtcagatga aaggctacaa
    15121 gcccgacgcg cctgagaaca atccaccctg gacgcagtgg tatgtgccat ttggccgcgc
    15181 tcacccaggc gacatctggg catttggcgc ctctggggac cggcccatcc atgaccttgt
    15241 cggcgtctcc tcccaggacc gcaaatggct ggccgctatt ggctgtcgcc ataccacgac
    15301 tctgggccaa ggctggcacg attgtatcca tcatgtgccg gatatgcaga agtacttgga
    15361 tgccggcgca agtcggattg tccatcgaag cgtgctgtat gtcatgccga atgacacagg
    15421 caaattgctc gaaagcttcc ggcaagattt cgaggcgaag ccggctgccg aagggatttc
    15481 tgtctcggtc gcgaatcatg gcaggttggc ggtctccact cgatcaccta ccgctactct
    15541 gttggacctg tccctcgtgg tggacaacgc gggaaggcgg catcgcgcgg tttggaaggc
    15601 atcatcatgg ggcggtcatg tgagcgactg tggcaccgca cgcctgtggg cttacgctca
    15661 cgatggggcg gttgaacttt gggccagcgc gccttcttcg atgcctccgg cccgcgtcga
    15721 tgcggcgctc ggaggtaagg gttggagtct gattcatgcg cccaaggacg ttcccgccat
    15781 cgtgcaacag tctccagacg gtgcctggac cgcggcgatg gtgtgggagc agagggaggc
    15841 gggtaacccg ggcacgggcg tcgctgctcc gggcacactc agctcggccg ctgcttcggt
    15901 tcgcgggcgg ctcctcatct tcaaggggag tgtggcagcg ctccaagaac gctgggtcga
    15961 gacgcaacag gaatggcggc actccatgcc ttaccgcatg cctccgcggg agcagaagga
    16021 aaaggtgcag gctggcgggg tacgctttgc gcccaatgcc gaaccggaca tgacgtacgg
    16081 tctgacaatt gtaccgcctt ggaaagacgg gggcacgtta cactccaatt tccccgagca
    16141 ccttgaacac ggcgacgtcg gatacggcat tcttcggcac cacaacaagc gaccctaccc
    16201 gtggagaatt gcagcggatg gccgctcggc agactacgaa gtagagagtc cggagttgcc
    16261 aggcgtgatc gttcgggcgt cggcggtagc caagggcgac ggtgcgcatc tgactctgag
    16321 aattacgaac gggggcaaca ggaggctgga gcgcgtcaaa ccgctcctgt gcttctggta
    16381 tgcgaaattg acgggttttc ccaccaggaa ctcggataac ttcgaacata catatgtcgt
    16441 cgtggacaac aagccaatcc cgctggccgg catcgcgaca gccaatccgg cggcgaccgc
    16501 caaggtggcc tacgtccacg gttgcagcca gcacgactgc gacaagttcg cacaaagccg
    16561 cggcgggttg atcgatcgtg atatcgacca cgccttgatt gtggtgacgg cgagggatgg
    16621 gaagagaaaa gtcgtcatca cctttgcgcc gggcaagagc atcctgtcaa acaagttcat
    16681 cccctgcgct cacgccgacc cgtactatgg aaccatagag ccaggcgcct cggcggaagc
    16741 aactggcgag gtctacttca ccgacaaacc gatcgcggag attgtgcgag cctttccacg
    16801 ccgctaacac tggaaagcgt gcaacgcttg atccagagct gtgtagacca ctactatacg
    16861 atgcggctgc ccagcaggat cgggtacgtg acgcgccaga ggccctaccc agtccggcaa
    16921 ctgcggagcc cgctagctgc tggccgggat atgcggcgat ttgtgtgtct gccacccctt
    16981 ggtcatgggg ccacggtgaa ctggagcgcg ttcgatcgcc ggccggtcag ggcgtcctcc
    17041 acatagatgg ggtacttgcc aggctttgcg tagagctccc tcggaacggt gcatgatacc
    17101 gcggtggctc ccgcaaccat cgatgggagc ctgcgatcgc cgagaatgac caccgacgag
    17161 cgcggtacgt tctccgtctg gagccagatg gccgacgtgc cgtcgggttg gacattaaac
    17221 cgttgcccag ccctcgtctg tagtggccct cccgctttga tcaccaagtc agcgaacttc
    17281 gcgaatgtcc cgtcgcgcaa aaagtagacg ggcgaaactt ccccgacgag gagcctcgcc
    17341 ttggtcagat cgaactcctg gacacggaac tcgaggtggg gtttgtttgc gagcgcctgc
    17401 accagcccgg gggtgctgaa cggatcgagc acccacttcc agtaccacac tatgctcggg
    17461 tccgcgacca tcagggtcag ggaccggatc ggccggtcct tctctggctc gatcatcgac
    17521 tggtaggcct gccgggcggc gttaccggcg ctgtaccaaa acagcccctc cacgttgcgc
    17581 cgcatcaccc aacagttcac cacgcacacc agcacgactg cggtccatag aacagtcagg
    17641 acgcgcctgg ggccttggct ccatgcgctc agcgagacag ccagggccat cgccaccccg
    17701 gccagtggca ccagcaagta gtacgtctgt gccaggccca ccagtagagt cggtgcgatg
    17761 aaggcgcccg tcaggcacag cccgctccac actcgggcat cccgaacgag cgcaggcacc
    17821 acggtcacca ggaacagcag cgctccgccg gccaggaagg tacgctccca ggtgtgaggc
    17881 gacggaagcc caaacagcgt cgcgaaactg ccggtaccgt ggatccacgg gtttgccagt
    17941 gagatgttga ggaagtcccc cgcatacaag acaaggtgag acagcgcccg gttgaggtcc
    18001 agcgtcggcg catagttccc ggcttgcgca aaaggcgttg tgcctcgcat ggcgatccag
    18061 cgcgtaataa aatacaccac cgtaaggcca ccatacgggc tcaacacgcg gaggagacgc
    18121 ctccaggaga acgcc
//
LOCUS       DATIFQ010000509         3767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1706718, whole genome shotgun sequence.
ACCESSION   DATIFQ010000509 DATIFQ010000000
VERSION     DATIFQ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706718"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VLE22_25735"
     CDS             <1..408
                     /locus_tag="VLE22_25735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17877.1"
                     /translation="ENRDQLWIFNLKTRRWENMEPKVLAPAGAQPPVCTRESVYLPGD
                     DVVLIYGPSRTERTKPALWEYNVAENSWRLVEIPPMTEVEPRQRASQNRAMVYDPKRD
                     LVLLVLGSRGDAGPAEVFAMRYRRSQAASASQE"
     gene            479..1360
                     /locus_tag="VLE22_25740"
     CDS             479..1360
                     /locus_tag="VLE22_25740"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSB17878.1"
                     /translation="MFYRGFLLFCCVSTMLAQPAARKLRVISFGAHPDDCDYRVGGTA
                     AKFARLGHQVKFVALTNGDAGHQSEGGGALARRRRAEAQEAGRRLGIASYEVLDNHDG
                     ELLPTLPVRHQVIRKIREWNADIVLAPRPWDYHPDHRYTGAVVQDAAYMVVVPNVCPD
                     TPALRKNPVFLYHEDEFQRPNPFRPDVAVDIGDTVEAKVSAFDAHVSQMYEWGPFLDG
                     ALASVPKDPVARREWLRKGWFSGTPAPAVLKALEKWYGPSAQTIRYAEAFEVCEYGHR
                     PTEQELRELFPFLPQTK"
     gene            1559..2068
                     /locus_tag="VLE22_25745"
     CDS             1559..2068
                     /locus_tag="VLE22_25745"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB17879.1"
                     /translation="MNRFTVVGLCLLMTLPFAAVGATLQDEALKHWKASKEFTIAVAE
                     MMPADGYDFKPNPEEMSFGELMIHMAVSQGNSFARASETKSPLSKPDPKDKAAVIKLL
                     NDSFDFAIQTLENLKPEQMERMSGKPGGPQSSGREILWSTFTHIAHHRGQAEVYLRVK
                     NIKPPQYRF"
     gene            2144..3274
                     /locus_tag="VLE22_25750"
     CDS             2144..3274
                     /locus_tag="VLE22_25750"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB17880.1"
                     /translation="MHTTDRRHLLRYLASLAAASAAGSRLYETAAAAPANVTGMRISR
                     YEIYRTQVPWAERVREMAILNWRRENMDVPHSPHTVVKLHTDEGLVGFGEGGNEATLK
                     SMVGHSPWEYVFDDSIGGAQTAIYDLLGKATGLPACHVLSATPKKRIIQAYWSLSYPP
                     DMLAKEAQLAASLGYRVHKVKIRPWEDPVAQAQALFSVVPRDYRVWGDANHFWGSVGR
                     TLYYAKKLAEFPGYFGLESPLRSTEGYRQLKGQVPLRLAEHWGLVDPMLAAREGVLDA
                     WISACPTFGPTMFRQSAFAQFYNIPLWDESSCWSGIGLAIQAQQAAAYPGIEYTINAA
                     VTAEDDLVKEPFVMKDGFYDIPQQPGLGVTLDEDAVDKYQVR"
     gene            3288..>3767
                     /locus_tag="VLE22_25755"
     CDS             3288..>3767
                     /locus_tag="VLE22_25755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17881.1"
                     /translation="MNTTSRRNWMAQSAAGGLGLGLAGAGAARGAPLRNEVKITRTEA
                     YNVRIPYATHLRENMLQNYRRENSNRPDYPTWIVKIHTNAGLVGVGEGGQDPRPRLAG
                     IEGRSVWELVHNVALGPAVMIAIYDLAAQATAVPVSRLFSPNPRPVIRQSWWSHSFRP
                     "
BASE COUNT          747 a         1118 c         1211 g          691 t
ORIGIN      
        1 gagaaccggg atcagctctg gatcttcaat ttgaaaaccc ggcgctggga gaacatggag
       61 ccgaaggtgc tcgcgcccgc gggagcgcag ccgccggtgt gcacgcgtga gtcggtctac
      121 cttcccggcg acgatgtcgt gctgatctac ggcccgtcgc gaacggagcg gacgaaaccg
      181 gcgctgtggg aatacaacgt cgctgaaaac tcttggcgtc tggtcgagat tccaccgatg
      241 accgaggtgg agccccgcca gcgggccagc caaaaccgcg ccatggtata tgacccgaaa
      301 cgcgatctgg ttctgcttgt gttaggcagc agaggggatg ccggaccggc cgaagtgttt
      361 gccatgcggt accggcgctc ccaggcggct tccgcgagcc aggaataagt tgctgctggt
      421 cgcactatcc aaaggagaat caagtttctg tggtacatag ggcgaaagga gagaacgagt
      481 gttctatcgt ggatttctgt tgttctgttg tgtctcgacc atgctggccc agccagcagc
      541 ccgtaagctt cgggtcatct cattcggcgc gcacccggac gactgcgatt atcgggtggg
      601 cgggacggcg gccaagttcg cccggctggg gcatcaggtg aaatttgttg cactgacgaa
      661 cggcgacgcc gggcatcagt ccgaaggcgg tggcgcccta gcaaggaggc ggcgggcgga
      721 ggcccaagag gctgggaggc ggctcggcat cgccagctac gaagtgctcg acaatcacga
      781 tggtgaactg ctgcccaccc tcccggtccg gcaccaggtc atccggaaaa tacgtgagtg
      841 gaatgcggat attgtgctag cgccgcgccc gtgggactac caccccgacc atcgctatac
      901 cggtgcggtg gtgcaggacg ccgcctatat ggtggtggtg ccgaacgtct gcccggatac
      961 cccggcactg cgcaagaacc ccgtgtttct gtatcacgaa gacgaattcc agcgcccgaa
     1021 tccgttccgt ccggacgtgg cggtcgacat cggcgacaca gtagaggcga aagtctcggc
     1081 gttcgacgcg catgtctcgc agatgtacga gtggggtccg tttctcgacg gcgcactggc
     1141 atcggtacct aaggaccccg tggcgcgacg cgaatggctg cgcaagggtt ggttctctgg
     1201 cacgcccgcg ccggcggtcc tgaaagcact cgaaaaatgg tacggcccca gtgcgcagac
     1261 gattcgctac gccgaagctt tcgaagtttg cgagtacgga caccggccca cggagcagga
     1321 gttgcgcgaa ctctttccgt tccttccgca gaccaagtaa acctggcgag agagactggt
     1381 tgccggcacc ggtggcggcc ctcgggccgt cgcggtcatg cgtagtctcc acccgaccgt
     1441 ccggaaacgc cggccgtcat tatggcgccg gcttctccgc cggcatatgg acccggttgc
     1501 gcactcattg ctatccaggc agttttgcgt tatctctgat agccgggagg gctaccccat
     1561 gaacaggttc accgtcgttg ggctctgtct tctgatgaca ttgccttttg ccgccgtagg
     1621 ggcaacattg caggatgaag cgctcaagca ctggaaggcg tccaaggagt tcaccattgc
     1681 ggtggctgag atgatgccgg ctgacggcta cgacttcaaa ccgaaccccg aggagatgag
     1741 cttcggtgag ttgatgattc acatggccgt ttcgcaaggc aattcgttcg ctcgcgcgag
     1801 tgagacgaag tcgccgctga gcaagccgga cccgaaggat aaggcggccg taatcaagct
     1861 gctgaacgac tcgtttgact tcgccatcca gacgcttgag aacctcaagc cagagcagat
     1921 ggaacggatg tctggcaagc cgggcggtcc gcagtcgagc gggcgtgaga tcctctggag
     1981 taccttcacg cacatcgctc accatcgcgg tcaggccgag gtttacctgc gcgtgaagaa
     2041 catcaagccg ccgcagtacc ggttctgatt ctacataggt cccgtccacc ggtacgagcg
     2101 ggagtggttc ttagaaaagc tttgcctctg aaaggggagc gctatgcata caacggaccg
     2161 gcgccacttg ttgaggtacc tggcaagcct ggcggctgca tcggcggcag gcagccgctt
     2221 gtatgaaacc gcggctgccg cgcccgcaaa cgtaactggg atgcggatca gccggtacga
     2281 aatctaccgg acccaagtgc cgtgggcgga gcgggttcgc gagatggcga tcctcaattg
     2341 gcgtcgggag aacatggacg ttccacactc tccgcatact gtggtcaagc ttcacacgga
     2401 tgaaggactg gtggggtttg gcgaaggtgg caacgaggcg acgctgaaat cgatggtcgg
     2461 gcattcgccc tgggagtatg tcttcgacga cagcatcggc ggcgcgcaga ccgccattta
     2521 cgatttgctc ggcaaagcaa ccggtctgcc cgcttgccat gtgctgtccg ccactcccaa
     2581 gaagcggatc atccaggctt actggagtct cagctacccc ccggacatgc tggcgaaaga
     2641 ggcgcaactg gccgcgagtc tcggataccg ggtccacaag gtgaagatcc gcccgtggga
     2701 ggacccggtg gcgcaagcgc aagctctctt ctcggtcgtt ccgcgcgatt accgcgtgtg
     2761 gggcgacgcg aaccacttct ggggctcagt cggtcggacg ttgtattacg caaagaagct
     2821 ggctgaattc cccggctatt tcgggcttga atcgccactg cgaagcacgg aagggtaccg
     2881 gcagctcaag ggacaggtac ctctcaggct tgctgagcat tgggggctgg tggacccgat
     2941 gctggcggct cgtgaggggg tgcttgacgc ctggatcagc gcctgcccca cgttcgggcc
     3001 cactatgttc cggcagagcg ccttcgccca gttctataac atcccgcttt gggacgaaag
     3061 cagctgctgg tccggcatcg gcctcgccat ccaggctcag caggcggcgg cgtatcccgg
     3121 catcgagtac acaataaacg ccgcggttac ggcggaggac gacctcgtca aggagccttt
     3181 tgtgatgaag gacggcttct acgacattcc gcagcagccg ggactgggtg tgaccttgga
     3241 cgaagacgcc gtcgacaagt accaggtccg ctaagggaga ggagcccatg aacacgacaa
     3301 gtcgccgcaa ctggatggca caatcggcag ccggtggatt agggctcgga ctggccggcg
     3361 ccggcgccgc gcgaggggca cccctccgca atgaagtgaa gatcactcgt accgaggcct
     3421 acaacgttcg catcccgtat gccacccacc tgcgggagaa catgctccaa aactaccggc
     3481 gcgagaacag caaccgtccc gactacccaa cctggatcgt caaaattcac accaatgccg
     3541 gtttggtggg cgtcggggaa ggggggcagg atcccagacc gagacttgcg ggaattgagg
     3601 gccgctccgt gtgggagttg gtgcacaacg ttgctctcgg cccagccgtg atgatcgcaa
     3661 tctatgatct ggccgcccaa gcgacggccg tgccggtctc ccgcttgttc tcccccaatc
     3721 cacgcccagt gatccgccag agctggtgga gccacagctt ccggccg
//
LOCUS       DATIFQ010000510        10908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2473800, whole genome shotgun sequence.
ACCESSION   DATIFQ010000510 DATIFQ010000000
VERSION     DATIFQ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2473800"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VLE22_25760"
     CDS             complement(<1..495)
                     /locus_tag="VLE22_25760"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HSB17882.1"
                     /translation="MFTGIIEEVGIVEVIETRAAGARLRLQCQAALDGTAEGASISVN
                     GVCLTAVDLKGNSFAADATPETMRRSNLGDLRRGSPVNLERPLSPGGRLGGHIVQGHV
                     DGTGELLLLEALGDGNWWLKVRAPRELERYLVEKGSVAIDGISLTIASVETGVVSVSV
                     IPHTY"
     gene            complement(499..1584)
                     /gene="ribD"
                     /locus_tag="VLE22_25765"
     CDS             complement(499..1584)
                     /gene="ribD"
                     /locus_tag="VLE22_25765"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HSB17883.1"
                     /translation="MSEALELARCGAGQTSPNPTVGAVLVRDDRVVGRGFHTWKGVRH
                     AEIVALEEARESARGATLYVTLEPCSHHGRTGPCTEALIKAGVSKVVAAMPDPNPLVS
                     GVGLRRLRSAGIEVEMAAQHSADAEQINEPFVHYMRKGQPLVTLKAALTLDGKISAPE
                     DNQGWITSERARAHVQELRHRSDAILTGIGTVLADDCLLTDRSGHERSRPLLRIVLDS
                     LLRIPPVARMVTTCNEDLLVVTTSAASPERYRALESRGVRILTLDGPGGRADMRRLIE
                     HLAREKYLSLMIEAGSKVNWAALEAGIVDKVFFYYAPKILGGLHSLPVAGGTGRRRRA
                     DAIVFRDVTLHRIPPDEFAVEAWLVKD"
     gene            complement(1596..2627)
                     /gene="ftsY"
                     /locus_tag="VLE22_25770"
     CDS             complement(1596..2627)
                     /gene="ftsY"
                     /locus_tag="VLE22_25770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HSB17884.1"
                     /translation="METYAIAAEGRRPGEGIAEGYNGKLAIMIQTLFGSAPKEPNLLD
                     RLKMGVQKTRAGLVSRLEDVLEGKKEIDADLLDELEYTLISADIGVKTTSEILERIRQ
                     RVDRHLVGDAGELRSLIREHLLEVLQGSETPPPRVSEPPFVVMVVGVNGSGKTTTIGK
                     LAQRYKAGGSTVLLCAADTFRAAAIDQLEIWGQRTATEVIRQKPGADPSAVMFDALQA
                     AKARKTDYVIIDTAGRLHTKSNLMAELEKMRRTASRVVSDAPHEVLLVLDATTGQNGL
                     EQARRFTETSGVTGIVLTKLDGTAKGGIVVAIARELSLPIRYVGVGEKPQDLLPFEPD
                     DFISSIFEA"
     gene            2626..4587
                     /locus_tag="VLE22_25775"
     CDS             2626..4587
                     /locus_tag="VLE22_25775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSB17885.1"
                     /translation="MSKPTRRNFLRSAGTAAALPVSATAVPTSPAKKAYRRSGFSPAG
                     APRKLTDNLYIFEDTCNVYIVRDGSSCVLIDFGSGKVLDHLRDLGISKVDWILHTHHH
                     RDQCQGDWKATERGIPVAVPAHERHLFGDARNFWRNRRVFHLYYVRNDFNTITEDIPV
                     ARPLADYSTLRWNKTDFYILPTPGHTLGAITLLASVDGKRVAFSGDLMHSPGKIHTLH
                     DTQVNYGGTEGIDLGIYSLARLREQKPEMLCPSHGTPLNDPDSGMAETIKRLTEYCRF
                     WGNEPVEENRPYAVSRHLVAHHQTTSSFYAILSGSGKAMFIDYGSASGLHFGNFMNAT
                     ATTDRIRFVEHSIDHLKSNFGMKSIDVAMPSHMHDDHMNGFPHLIRHHGTKIWCYENM
                     VDIFENPRGHNLGCILGEPFKVSRSFKNGEKFKWEEFELEVAHSPGHTEYQMALFVNI
                     DGNRIAFTGDAIFENPRKDGTLRHNLIFRNHVENDSHLKSIRAIIGREPNMIAPGHGK
                     PYAVDQPTLLATEQKLRKGQQHYFDLLPEGEVGYGLDPSWVKIYPYQILLTPGQPQSL
                     EIRVQNYNPSAMKLEAAIVAPSEWRIEPDVVKLDIPAQSHGVGKVTVTAPRDWDAPSA
                     RFAIAADVVRDRKYLGQITEAVVEMASNT"
     gene            4723..6657
                     /locus_tag="VLE22_25780"
     CDS             4723..6657
                     /locus_tag="VLE22_25780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSB17886.1"
                     /translation="MERIGRYRIDSELGKGAMGIVFRAQDPAIGRTVAIKTIRLTEFV
                     DPVERGRLRERLLNEARSAGILSHPGIVTVYDAGEENETAYIAMECVNGPSLEQLLSG
                     DTPVDPDTLLRVIRETAAALDYAHKKGIVHRDIKPGNIMLQDDGTVKITDFGIAKIAS
                     QQLTQAGTVLGTPNYMSPEQVRGKKVDGRSDQFSLAVIAYEMLTGDRPFTADQLPTLL
                     FKIVHDEPPPVQRFNPTLTWQIEVVLRKALEKEPDQRYRNCAEFAAALDAACRSSKDW
                     KPLPRGGDDDLPTVTTKIVEQPEAEPEPAAAPEALAPPPAAAAPQVPAIAQPDVVPEV
                     QAEPQPVTEPTPGPQPVQPAPPRAEPPPAAGAPPPRVEPERKAPPPRKREPEPAGVIV
                     AEEVVPEKSRTMLFVAGGIAAVLVIAGIAGWSMGWFGGQQTVQTAQTSAPPPQPPPSS
                     PVETSSRPSPAASRAKQNPPDVPPAPAATPATQEMEQAPGRIRQPAEPPVTPGAGSET
                     AVNVQTVPPGATVTFDNNPAFTCQSPCSLPLTNGRHTLSATTGGYRTALRIFELPRDA
                     ELSVNLIRMAGQILIVTEPPGAAILVNGKQRSEKTPATIELPVGKQTVVVSMPGYKND
                     DQEIDVKDGAYMRLSFTLSK"
     gene            complement(6726..8834)
                     /locus_tag="VLE22_25785"
     CDS             complement(6726..8834)
                     /locus_tag="VLE22_25785"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HSB17887.1"
                     /translation="MASTRIGSQVDVELLGRLGGIEPDTLLRAFRVMLLSRRIDDREI
                     LLKRQSRVYFQINGCGHEAVSAAAGMALRPGHDWFYPYYRDRAMCLALGVTAYEMLLA
                     SVGAAGDPASGGRQMPSHWGSQRLNIVSQSSPTGTQFLQAVGCAEAARLLNPESDEVT
                     VVTAGDGATSEGEFWESLNIACLEKLPVLILIEDNQYAISVPIEKQTAGGSISKLVRD
                     FPGLLCLEVDGCDFVASHNTLREAAEWCRLRKGPALVHAHVIRPYSHSLSDDERLYRP
                     RSEREADAERDPIAAFPKWLVAEGVADPRTLQRIAHEVEEEIQEATEKALQESAPAPA
                     TAQLYLYSDKVDPTSDEFSTPAQFSGPPRTMVDEINLTLAEEMRRDPRVVVFGEDVAD
                     CGRERHLREVKGKGGVFKATLGLQTEFGNQRCFDTPLAEAAIVGRAVGLATRGVKPVA
                     EIQFFDYIWPAMMQIRDELATMRWRSSNAFSCPAVIRVAIGGYLTGGAMYHSQCGEVL
                     FTHIPGLRVLMPSNSLDACGLLRTAIRCDDPVLFLEHKRLYREPYNRAPHPGPDFLIP
                     FGKARVVKAGRDLTVVTYGAVVQKSLQAALQIEKKSSQVSIEVIDLRSLAPYDWGAIR
                     ESVEKTSRVIIAHEDCLSFGYGAEIAARIAGELFDRLDAPVGRVAALDTWVGYNPELE
                     NAILPQVEDLAQEMERLLAY"
     gene            8946..10298
                     /locus_tag="VLE22_25790"
     CDS             8946..10298
                     /locus_tag="VLE22_25790"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB17888.1"
                     /translation="MPADVITKSGFEWATVFRALRHRNYRLFIGGQFISLIGTWMQNV
                     AQSWLVYRLTGSSLLLGLIGFVNQVPIFLLSPAGGAAADRVDRRRLIIAAQFSMMVLA
                     TILAALTLGGWIRMWHIFVLAALLGVANAFDVPARQSFFVQLVGRTDLMNAIALNSSV
                     FNGARILGPAVAGFLVASLGEGWCFLLNAVSYVAVIAGLLLIRLDTRPVREPGSSPLA
                     DIIEGFRFVWHTGPIHALLALLGVVSLVGMPYTVLMPVFADRVLEGGAKSLGLLLGAT
                     GIGALSGALMVAARRGVKGLGRWVAIACAAFGTFLLLFSLSRQLWLSMILLVPAGFAV
                     MVQMACSNTLIQSMVPDHLRGRVMALYSMTFLGIAPFGALLAGALADRLGAPVTVAVG
                     GLVCLAGAAIFWTRWPALRVAARELVVAQQLSAGAPPEEITSAASPEGPSIAKSESGA
                     "
     gene            complement(10312..>10908)
                     /locus_tag="VLE22_25795"
     CDS             complement(10312..>10908)
                     /locus_tag="VLE22_25795"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17889.1"
                     /translation="GEDMMVLGTHIFDFFRMFAGDPKWVFAHVTHDGAELDRSRVRTP
                     SEPVGPVAGSQIAAMFAFENGLHGYFASHANEGDGVARYGVTFYGSKGAIAFPMDSYP
                     GGNPYLLRSASWLPERPGAAWQKIEVLPEEQVDTREKANAMMVADLLEAIEQDREPAC
                     GARDGRWTIEMTAGAYQSQKTGMRTFFPLKDRKHPLAS"
BASE COUNT         1964 a         3758 c         3183 g         2003 t
ORIGIN      
        1 atacgtgtgc gggatgacgg acaccgagac gacgccggtc tctaccgagg cgatggtcag
       61 actgatgccg tcgatagcca cggaaccctt ttcgacaaga taccgttcca gttcgcgagg
      121 tgcgcggacc ttgagccacc agttcccgtc tccgagcgcc tccagcaaca gcagctcgcc
      181 cgtgccgtcc acgtggccct gtacaatgtg cccgccgagc ctcccgcccg gtgacagcgg
      241 acgctccagg ttgaccggcg atccgcgccg caggtcgccg agattgctgc ggcgcatcgt
      301 ttcgggagtt gcgtccgccg cgaacgagtt ccccttcagg tccaccgccg tcaggcagac
      361 gccattgacg gagatgctcg caccctcagc cgtgccgtcg agcgccgcct gacattgcag
      421 gcgaagccgc gcgcccgccg cgcgtgtttc aatcacctcg acaattccga cctcttcgat
      481 gatcccggtg aacatgactc agtccttcac cagccaggcc tcgacggcga actcgtcagg
      541 gggaatccgg tggagcgtca cgtcccggaa aacgatggcg tcggcgcgcc gacggcgccc
      601 cgttcctccc gccaccggaa gcgagtgcag gccaccgagg atcttgggag cgtagtagaa
      661 gaaaaccttg tccacgatcc cggcttcaag cgccgcccag ttcaccttgc tccccgcttc
      721 gatcatcagc gacaggtact tctcccgtgc cagatgctcg atcagccgcc gcatgtcggc
      781 ccggcctccg ggtccgtcga gcgtgaggat gcgcacgccc cggctctcga gcgcccggta
      841 tcgctccggc gaggccgccg acgtggtcac cacaagcagg tcttcgttgc acgtcgtcac
      901 catgcgcgcc acgggcggaa tgcgaagcag cgaatcgagc acgatccgca gcaacggccg
      961 gcttcgctcg tgtccggagc ggtccgtcag caggcagtcg tcggcgagca cagtcccgat
     1021 acccgtcaga atggcgtcgg agcggtgccg cagctcctgc acatgagcgc gcgcccgctc
     1081 gctcgtgatc cagccctggt tgtcctccgg cgcggagatc ttgccgtcca gcgtaagcgc
     1141 cgccttcagc gtcaccagcg gctgtccctt ccgcatgtag tggacgaacg gctcgttgat
     1201 ctgctccgca tccgcggagt gctgagccgc catctccact tcgatgcccg ccgaacgcag
     1261 ccgccgcaag ccgacgcccg aaaccagcgg attcggatcc ggcatcgccg ccaccacttt
     1321 gctcacgccc gccttgatca gcgcctcggt gcagggcccg gtccggccgt ggtgcgaaca
     1381 cggctcgagc gttacgtaga gcgtcgcccc gcgggcgctc tcgcgcgcct cctccagtgc
     1441 gacgatttcc gcgtgcctga cgcccttcca tgtgtgaaaa ccccggccca ccacgcggtc
     1501 gtcacgcaca aggacggcgc cgaccgtcgg attcgggctg gtctggccgg cgccacacct
     1561 ggccaattcc agcgcctcgc tcatgtagtc cacggctatg cctcgaagat ggaagagatg
     1621 aagtcgtcag gctcgaacgg cagaaggtct tgaggcttct caccgacgcc cacgtaccgg
     1681 atcggcaggc tcagctcccg cgcgatcgcc accacgatgc ctcccttggc cgtaccgtcg
     1741 agcttcgtga gcacgattcc tgtcactccg gaggtttctg tgaaccgccg cgcctgttcc
     1801 aggccgtttt ggcctgtcgt ggcgtccaga accagcagca cctcgtgcgg tgcgtccgac
     1861 accaccctgg aggcggtcct ccgcatcttt tccagttctg ccatcaggtt ggatttcgtg
     1921 tgcaggcggc cggccgtgtc aatgatcacg tagtccgtct tgcgcgcctt cgccgcctga
     1981 agtgcatcga acatcaccgc gctcggatct gctcccggct tctggcggat cacctcggtg
     2041 gcggttcgct gtccccagat ttcgagctga tcgatcgccg ccgctcggaa cgtgtcggcc
     2101 gcgcacagca gcacagtgct tccgcccgcc ttataccgct gggcgagttt gccgatggtg
     2161 gtcgtcttcc ccgagccgtt caccccgact accattacca cgaacggagg ctcggagacc
     2221 cgcggcggcg gcgtttcgct gccctgaagg acttcgagaa gatgctcgcg gatcaggctc
     2281 cggagttccc cggcgtcgcc gaccagatgc cgatccacac gctggcggat gcgttccagg
     2341 atctccgacg tcgtcttcac cccgatatcg gcggagatca gcgtgtactc gagttcgtcg
     2401 aggaggtccg cgtcgatctc cttcttgcct tcgagaacgt cctccaggcg cgacaccagg
     2461 cccgcgcgtg tcttctggac acccatcttc aggcgatcga gcagattggg ttccttcggc
     2521 gcggaaccaa aaagagtctg aatcatgatg gccagctttc cattgtagcc ttcggcgata
     2581 ccctcccctg gtcttcggcc ttcggccgcg atagcatacg tttccatgag caagcccacc
     2641 cgccgaaact ttctgcggtc ggccggcacc gccgccgcgc tgcccgtctc cgcaaccgcc
     2701 gtcccgacga gtccggcaaa gaaggcgtac cgccggtccg gattcagccc ggccggcgct
     2761 ccccgcaagc tcacggataa cctgtacatc ttcgaggata cctgcaacgt ctatatcgtt
     2821 cgggatggct cgagctgcgt cctgatcgat ttcggctcgg gtaaagtcct cgaccatctc
     2881 cgcgacctcg gcatctccaa ggtggactgg attctccaca cgcaccacca ccgcgatcag
     2941 tgccagggag actggaaagc caccgagcgc ggcatccccg tcgccgtccc tgcgcatgaa
     3001 aggcacctct tcggcgacgc gcgcaacttc tggcgcaacc gccgggtttt ccatctttac
     3061 tacgtgcgca acgatttcaa caccattacc gaggacattc cggtcgcccg cccgctcgcc
     3121 gattactcta ccctccgctg gaacaagact gatttctaca tcctgccgac tcccggccac
     3181 actctcgggg cgatcacctt gctggcgagc gtggacggga agagggtcgc attctccggc
     3241 gacttgatgc actctcccgg caagatccac acgctccacg acacacaggt caactacggc
     3301 ggcacggagg gaatcgatct cggcatctac tcgctcgccc gccttcgcga gcagaagcct
     3361 gagatgctct gcccatccca cgggacgccg ctgaacgacc cagactccgg aatggccgag
     3421 accatcaagc gcctgaccga atactgccgc ttctggggga atgagccggt cgaggagaac
     3481 cgtccgtatg ccgtaagcag gcatcttgtc gcgcaccacc agacgacgtc gtcgttctac
     3541 gccatcctca gcggcagcgg gaaggcgatg ttcatcgact acggttccgc gtcgggtctc
     3601 catttcggca acttcatgaa cgccaccgcc accaccgacc ggattcgctt cgtcgagcac
     3661 tcgatcgacc acctgaagag caacttcggc atgaaatcga tcgacgtcgc gatgcccagc
     3721 cacatgcatg acgaccacat gaacggcttt cctcacctca ttcggcatca cggaacgaag
     3781 atctggtgct acgagaacat ggttgacatc ttcgagaacc cgaggggcca caacctcggc
     3841 tgcattctcg gcgagccctt caaggtttcg cgctcgttca agaacggaga gaagttcaag
     3901 tgggaggagt tcgaattaga ggtcgcccac tcgcccggtc acaccgagta ccagatggcg
     3961 ctgttcgtca acatcgacgg aaaccgcatc gctttcaccg gcgatgccat cttcgagaat
     4021 ccgcgcaagg acgggacctt gcggcataac ctgattttca ggaaccatgt cgagaacgac
     4081 agccacctga agtcgatccg ggccattatc gggcgcgaac cgaacatgat cgcgccaggc
     4141 cacggtaagc cctacgccgt cgatcagccg accctgctgg caaccgaaca gaaactgagg
     4201 aagggacagc agcactactt cgacctgctg ccggaaggtg aggtcggcta cggcctcgat
     4261 ccgagctggg tgaagatcta cccgtatcag atcctcctga cgcccggcca gccccagtcg
     4321 ctggaaatcc gcgtccagaa ctacaatccg tcggcgatga aactggaggc ggcgattgtg
     4381 gccccctcgg aatggcgcat cgagcctgac gtagtgaaac tggacattcc ggcgcaatcc
     4441 cacggcgtgg gcaaggtgac tgtaaccgct ccccgggatt gggacgctcc cagcgctcgc
     4501 ttcgccattg ccgccgacgt cgttcgagac cgcaagtacc tcggccagat caccgaagcc
     4561 gtagtggaga tggcgagtaa cacataggct gccggcccga ttctcgggac ggcgccacga
     4621 tgcgacgcaa cccacggatt tagaaggatt tcagtcactt ctctcctttt tccgctgtgc
     4681 actggaggtg cggggattgc tgcgctacaa taacagcacc gcatggaacg gatcggcagg
     4741 tatcgaattg actctgaact gggtaagggg gcaatgggca tcgttttccg ggcccaggat
     4801 cccgccatcg gccgcacagt cgccattaag accattcgct taaccgagtt tgtcgacccg
     4861 gtcgagcgtg gccgcctccg ggaacgcctc ctcaacgaag cgcgctccgc tggaatcctc
     4921 tcgcaccccg gcatcgttac ggtctatgac gcaggcgaag agaacgagac ggcctatatc
     4981 gccatggagt gtgtcaacgg cccctcgctc gaacagcttc tctcgggcga cacgcctgtc
     5041 gatccggaca cgctgctgcg ggtgattcgt gagacggccg ccgctctcga ctacgcccac
     5101 aaaaaaggta tcgtccaccg tgacatcaaa ccgggcaaca tcatgcttca ggacgatggc
     5161 acggtcaaga tcacggattt cggcattgcc aagatcgcct cgcagcagtt gacccaggcg
     5221 ggaaccgtcc tcggcactcc caactacatg tcgccggaac aggtgcgcgg caagaaagtg
     5281 gacgggcgct ctgaccaatt ttcgctggcg gtgatcgcct atgagatgct gacgggcgac
     5341 cggccgttca cagcggacca gctaccaacg ctgctcttca aaatcgttca cgatgagccg
     5401 cccccggttc agcggttcaa tccgacgctt acatggcaga tcgaagttgt tcttcgcaag
     5461 gcacttgaga aggagcctga ccaacggtac cgcaactgcg cggaatttgc agcagcgctg
     5521 gatgccgcct gtaggtcctc caaggattgg aagcctctgc cgaggggcgg cgacgacgat
     5581 ctgcccaccg tcaccactaa gatcgtcgag cagccggagg ccgaaccgga acccgcagcc
     5641 gctcccgaag ctcttgcgcc gcctccagcg gcagccgcgc cacaggtacc cgccatcgcc
     5701 cagccggatg tcgtaccaga ggtgcaggcc gagccgcagc cggttaccga gccgacgccc
     5761 ggtccgcaac cggttcagcc tgcgccgcca cgcgcagagc ctccaccggc ggccggggct
     5821 ccaccgccac gggtcgagcc ggagcgcaaa gcgccacctc ctcggaagcg tgagcctgag
     5881 ccggccgggg tgatcgtggc ggaggaggtt gttccagaga agtctcggac catgctgttc
     5941 gtggccggtg ggatcgccgc tgttctcgtc atcgccggga ttgccggctg gagcatgggt
     6001 tggttcggcg gacagcaaac cgtacagacc gcccagactt ccgcgccccc cccgcaaccg
     6061 ccgccttcct cgcctgtcga gacctcgtct cgtccgagtc cggcagcctc ccgggcaaag
     6121 cagaaccccc cggacgttcc gcctgcgccg gcggcgacac ccgcgacaca agagatggag
     6181 caggctccgg gcaggattcg acaaccggcg gagccgccag tgacgccggg cgccgggagc
     6241 gaaaccgcgg ttaacgtcca gacagtcccg cccggcgcga ccgtcacctt tgacaacaac
     6301 ccggcattca cctgccaaag cccttgctct ttacctctca ccaatggccg gcatacgctc
     6361 tccgccacga cgggcggtta ccggactgcg ctccggattt ttgagttgcc acgagatgcg
     6421 gaactctccg tcaatctcat ccgaatggcc ggacagattc tcatcgtgac cgaaccgccc
     6481 ggcgctgcaa tcctggtcaa cggaaagcag cgtagtgaga aaaccccagc aaccatcgag
     6541 cttccagtcg gcaagcaaac cgtggtcgtc agtatgccgg gatacaagaa tgatgaccag
     6601 gagatcgacg tgaaagacgg cgcctacatg cgcctgagct tcactctctc caagtagcgg
     6661 atcaaggcgc tccaggcagg gtgctgaaca aggttctgtg gggcgccgct ccgccactac
     6721 caggcttagt aagcgagaag ccgctccatc tcttgcgcaa ggtcctccac ttgtggcagg
     6781 atcgcgttct ccagctccgg attgtagccg acccacgtgt cgagagcggc cacccttccc
     6841 accggggcat ccagcctgtc gaatagctcg cctgcgatac gggcagcgat ctccgcgccg
     6901 tatccgaacg acaggcaatc ctcatgcgcg attatcaccc ggctcgtttt ctcgacggac
     6961 tcgcggatcg ccccccagtc gtatggagcg agactccgaa ggtcgatgac ttcgatgctc
     7021 acctgcgagc tcttcttctc gatttggagc gccgcctgga gcgacttctg caccaccgcc
     7081 ccatacgtaa ccaccgtaag gtcgcggccg gccttcacca ccctggcctt cccgaacggg
     7141 atcagaaagt ccggccccgg gtgaggagcc cggttgtaag gttcccggta caggcgcttg
     7201 tgttccagga aaaggaccgg atcgtcacac cggatcgccg ttcgcagcag cccgcatgcg
     7261 tccagggagt tcgaaggcat cagcaccctc agcccgggga tgtgggtgaa cagcacttcg
     7321 ccgcactggc tgtggtacat ggctccgccc gtcaggtagc caccgattgc cacacggatc
     7381 accgccggac acgaaaacgc attactggat cgccagcgca tcgtggccag ttcgtcacga
     7441 atctgcatca tcgccggcca gatgtagtcg aagaactgga tttcagcgac gggcttcacg
     7501 ccccgcgtcg ccagcccaac agctctgccc acaatggccg cttcggccag cggcgtgtcg
     7561 aagcaccgct gatttccgaa ctccgtttgc agaccgagcg tcgctttgaa aaccccaccc
     7621 ttacctttga cctcgcgaag gtgccgctcg cgcccgcagt cggcgacgtc ctccccgaat
     7681 acaaccaccc gaggatcgcg gcgcatctct tcggctagag tgagattgat ctcgtcgacc
     7741 atggtccgcg gcggccctga aaactgcgcc ggcgttgaga actcgtccga ggttggatcc
     7801 accttgtcgg agtataggta gagctgggct gtcgccgggg ctggggccga ctcttggagc
     7861 gccttctccg tcgcttcttg aatctcctcc tccacctcgt gcgcgatccg ctggagagta
     7921 cgggggtccg ctacgccttc tgccaccagc cacttgggaa aagcagcgat cggatcgcgc
     7981 tccgcatccg cctccctctc cgatcgaggc cggtagaggc gctcgtcatc cgacaatgag
     8041 tgcgaatacg gccggatcac gtgtgcatga accagtgcgg gacccttgcg caaccggcac
     8101 cattccgccg cctcgcgcag cgtgttgtgc gacgcgacga aatcgcatcc gtccacttcc
     8161 agacacagca accccggaaa gtcgcgcacc aacttcgaaa tgctgcctcc cgcggtctgt
     8221 ttctcgatcg gcacggagat ggcgtattgg ttgtcctcga tcaggatcag cacgggaagc
     8281 ttttccaggc acgcaatgtt cagcgattcc cagaactcgc cctcactggt ggcgccgtca
     8341 ccggcggtga ccaccgtcac ctcgtcgctc tccggattca ggagtcgcgc cgcctccgcg
     8401 catcccaccg cctggaggaa ttgtgttccc gtcggcgacg actggctcac gatgttcagc
     8461 cgctgtgagc cccagtgcga gggcatctgc ctaccgccgg aggccgggtc gccggctgcg
     8521 cccaccgaag cgagcagcat ctcgtacgca gtcacgccga gcgccaggca catcgcgcgg
     8581 tcccggtagt acggatagaa ccagtcgtgc ccgggccgaa gcgccattcc cgccgcagcg
     8641 gagacggcct cgtgcccgca gccgttaatc tggaagtaca cacggctctg ccgcttgagg
     8701 aggatctccc ggtcgtcgat ccgccgggac aggagcataa cccggaacgc gcgcagcaac
     8761 gtgtccggct caatgcctcc caaacggcct agcagttcta cgtccacctg cgatccaatg
     8821 cgtgtgctcg ccaaggcaca ccccccacac caagtgtaat ccgggtcatc cgccgcggga
     8881 agagacggtg agggcaaggt agcacgccgg ctttgcagcc actgcataat gaagagacga
     8941 gctgcatgcc ggcggacgtc atcaccaaat cgggattcga gtgggccacg gtctttcggg
     9001 cgttgcggca tcgaaactac cggcttttca tcggtggcca gttcatctcc ctgatcggga
     9061 cctggatgca gaacgtcgcc caatcctggc tggtctaccg gctcaccggc tcttccctgc
     9121 ttctcggcct gatcgggttc gtcaatcagg taccgatctt tctgctctct cccgccggcg
     9181 gagccgcggc cgatcgagtt gatcgccgcc gcctgatcat cgccgcccag ttctccatga
     9241 tggtgctggc cacgatactc gccgcgctca cgcttggcgg atggattcgc atgtggcaca
     9301 tcttcgtgct ggcggcgctc cttggagtgg ccaacgcctt cgacgttccg gcccggcagt
     9361 cgttctttgt gcagttggtc ggcagaaccg atctgatgaa tgccatcgcc ctgaactctt
     9421 ccgtatttaa cggcgcgcga attcttggcc cggcggtggc cggtttcctg gtcgcgtcgc
     9481 tcggcgaagg ttggtgcttc ctgttgaacg ccgtcagcta cgtggccgtg atcgccgggc
     9541 tcctcctgat acgcctcgat acccgccctg tccgggaacc cggcagttca ccccttgcag
     9601 acatcatcga aggctttcga ttcgtctggc acacaggccc catccacgcc ctcctcgccc
     9661 tgctgggcgt ggtcagccta gtggggatgc cgtacacagt actcatgccg gtgttcgccg
     9721 atcgtgtcct ggaaggcggc gcgaaaagcc tgggcctcct cctcggcgcg accgggatcg
     9781 gagcgctttc cggcgccctc atggtcgccg cccgccgcgg tgtcaaggga ctcggccgct
     9841 gggtcgcgat cgcatgcgcc gcattcggaa cctttctgct tcttttctcc ctcagccgcc
     9901 aattgtggct ctctatgatt ctcctcgtac cggccggctt tgccgtgatg gtgcagatgg
     9961 cctgctccaa tacactcatc cagtcgatgg tcccggatca cctgcgcggt cgagtaatgg
    10021 ctttgtattc aatgactttc ctgggaatcg cgccattcgg agcgcttctg gccggcgccc
    10081 ttgcggaccg gctcggagcc cctgtgaccg tggctgtagg cgggctggta tgcctcgctg
    10141 gagcagcgat tttctggacc cgctggccgg ccctccgcgt cgctgcccgt gaattggtcg
    10201 tcgcgcagca gctttccgcc ggcgcccctc cggaggagat caccagcgcc gcaagcccgg
    10261 aggggccctc catcgcgaaa tctgagtccg gagcgtaagc ccctcgtccg ttcaggatgc
    10321 cagcgggtgt ttcctatcct tcagcgggaa aaacgtcctc atccccgtct tctgtgactg
    10381 gtacgcaccc gccgtcatct cgatcgtcca gcggccgtca cgcgcgccgc aggccggttc
    10441 ccggtcttgc tcgatcgcct cgagcaggtc ggcgaccatc atggcgttcg ctttctccct
    10501 ggtatcgacc tgctcctctg gaagaacctc gatcttctgc caagccgcgc ccggccgctc
    10561 cggcagccat gatgcggagc gcaggaggta ggggttccct cccgggtagg agtccatcgg
    10621 aaacgcgatg gcgcccttgc tcccgtaaaa cgtcaccccg tatcgagcca ccccatcgcc
    10681 ttcgttcgcg tgcgaggcaa agtacccgtg caagccattt tcaaaggcga acatggctgc
    10741 gatctggctc cccgcgaccg gcccgacagg ttcggaaggc gtgcggacgc ggctccggtc
    10801 caactccgcc ccgtcgtgcg tgacgtgcgc gaatacccac ttcggatctc ccgcgaacat
    10861 ccggaagaag tcgaagatgt gcgtgccgag caccatcatg tcctctcc
//
LOCUS       DATIFQ010000511         9667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_751749, whole genome shotgun sequence.
ACCESSION   DATIFQ010000511 DATIFQ010000000
VERSION     DATIFQ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751749"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VLE22_25800"
     CDS             complement(<1..278)
                     /locus_tag="VLE22_25800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17890.1"
                     /translation="MSPPFRLLVLACGFSGMCWAARCTTVLEPLALDAYEDYVLAAEK
                     AASARFAARELAWIPDSASAEAGARLDSGRVIRQKLSDAAVNQRIAGQ"
     gene            complement(275..2191)
                     /locus_tag="VLE22_25805"
     CDS             complement(275..2191)
                     /locus_tag="VLE22_25805"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17891.1"
                     /translation="MASPQSRCYEFGPYRIDTRERLLHRGEEVVSLPPKVIATLLALL
                     ENPGRMVDKGDLMKTVWPDVYVEEGALARNVSLLRKTFGDVGQEPVYIETFHRRGYRF
                     VAPVRTAALETDSPAAGETLSATPADAVQLQPSPEGMRRNRLVLAGALLLVLAGVAAL
                     TVYLFRSRPGSLSAPVRTPRTTLAVFSFRSLETGPGQDYFAEGMTQALVTALAKLGSL
                     RVISLASESALREKPAALSKVLRDQSVNRILTGTVLRSGQRVRIDAQLIDPGTRAVYW
                     ANYYERDMTDVLTLEAAVAEAIAGEIQVTITAEEKRNLRTNRTVNPEALDAYLRGRHF
                     WNRRTEDGLRRAVQYFQQAIALDPAYALAYSGLADSYALLGSIGVDGMPPVKAMPLAK
                     TAAQRALELEPNLADAHTSLAYVKLSYDWDLQAAEKEFSRALALNPNSATAHHWFSHY
                     YMAAGDLSKAAEQMREALRLEPLSPGINIGIGWCHYYARRYDEAIGQYRSVVDLEPRL
                     PLAHQTLGMAYQQKGMFSEAIDEFQQAVALSGGSPGPVAALASAYAAAGKIADARKEL
                     ARLEEMSRTRYVPAFYLASIYYSMGDVARALQLGWKAVGERCDYLMYLRVEPRAGKLA
                     ANPEFIRLVAALHR"
     gene            2379..2915
                     /locus_tag="VLE22_25810"
     CDS             2379..2915
                     /locus_tag="VLE22_25810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17892.1"
                     /translation="MRMAIVAVALLMAGCTASLHPLYSEDDAVFDPMLLGTWRESEGE
                     KFFVSVRAENAAYRVIYYDGEGSGAFKARLVELQGNRFVDLYPETPPSKNGFYNVHLI
                     PAHSFGRILLAGDRLTITLLDADWLRSEQASQVLLERISLDDCQVISAATADLRRFAT
                     KYASTKAFSVTSEWVREQ"
     gene            3063..3935
                     /locus_tag="VLE22_25815"
     CDS             3063..3935
                     /locus_tag="VLE22_25815"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HSB17893.1"
                     /translation="MKRMAVNKIGNALASGGSTYESDEDLELVGDALPFGLKLIESLL
                     AESPRHRALLQSACQGFTTYSYLYVQQEADRLADNDLDAADRLRARARRLFLRAHRYG
                     FRLLETTAPNVSAQMTDDPKGATLRFRKRTEVPSLYWNAAALGLAIAVSKRDAAMLAR
                     LPEVEALIGRAVELDDTWGDGALHEFEVILAGAKPGKPDFDAIEVHYQRALTLSKGKR
                     ASVHVAYAESVSVPRQDREGFRASLEKALAVDPDQYPEVRIPNLAAQRRARWLLERVD
                     ELILPPVAALEEEK"
     gene            3932..4933
                     /gene="dctP"
                     /locus_tag="VLE22_25820"
     CDS             3932..4933
                     /gene="dctP"
                     /locus_tag="VLE22_25820"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HSB17894.1"
                     /translation="MKTKFFLVILFAWSALFAQKLQVNVGTAAPEGSPWHEILQKTRQ
                     DWNRISGGNLTVRIYPSGVLGDEVEMIRKARIGQLQAVAVSGVGLSHIEPAAGCLQIP
                     MLMGSYEEFDYVRERIAPRLESLIAQKGFIVLQWSDVGWVHFFTKKPAKTPDDIRKLR
                     LFTSAGDPEGERLYKEFGLQVVPLAVTDMLPSLQTNLIQAFDVPPLFAMLDQSFGLAP
                     NMIDVKWTPLAGATLLSRKTWDRIPAEQRTELLRASRSASAGVRAQVRKMGEDAIVEM
                     QKRGLNVIKLDPATLSKWQVEAKSSYPKIRGEVVPADLFDEVVRLHNEYLSRMGGKG"
     gene            4933..6741
                     /locus_tag="VLE22_25825"
     CDS             4933..6741
                     /locus_tag="VLE22_25825"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HSB17895.1"
                     /translation="METCRTPTTRIEACASIALLGLMAALPLAEIAGRKIAGHGIPGS
                     IPIVQHLTLWITFLGAAMAVRSDRMLALSTATFVSETWRARIRVLTSTVAVAITLCLA
                     AASLELARVEREAGDLVAWGIPVWIAIAIMPAGFALIGFRLIWSAAPHWKGRAAAATG
                     LAIPLLFHSVSASRSADLTVGAVIAILGGAALGMPIFAVLGGLALLLFWNAGVSAAAV
                     TADIYRLAASPMLPAIPLFTLGGYLLSEGGAGKRLMRTFTALVGWMPGGLAVVTTLLL
                     AFFTPFTGASGVTILSMGGLLLPMLVKARYPEGTSIGLVTVAGSIGLLLPPSLPVILY
                     GIYAQTPIDRLFVAGLIPGFLLIVAVAGWGAWRGWFSGAQRVRFDAREAAAATWDAKW
                     ELLLPVVVLAGMLGGFATLVEAASLTVLYALIVECLVYRDLRVRSDLPRIASECGTLV
                     GGFLIILGVALGFTNFLIHAEIPMQALTWVKLHIHSKLVFLLALNGLLLVVGALMDIY
                     SAILVVVPLITPMAAAYGVHPLHLGVIFLANMELGYLTPPMGENLFLSSFRFGRPLSQ
                     VYADTLPYCAMLLATVLLITYAPWITMLPISFLEGG"
     gene            6738..7352
                     /locus_tag="VLE22_25830"
     CDS             6738..7352
                     /locus_tag="VLE22_25830"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSB17896.1"
                     /translation="MNFTKLAVILVLGAMTPRAFSQSSFEIQPFAGFRFGGTIRTADD
                     DPLPIKVGSSPVAGATFTYGFDESFGLEFLWAHQSTRGMTAVQKANLRLDQFLVHVVL
                     NMREREKSLQPFLLGGAGATHASGATSISTKVAWSVGGGIKYFVSRHFGFRLQARYAP
                     TELYSTPLPDSDSYCSGITYCGDPKKQHDLSQGEVTAGWIFRFP"
     gene            7676..8077
                     /locus_tag="VLE22_25835"
     CDS             7676..8077
                     /locus_tag="VLE22_25835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17897.1"
                     /translation="MLGEQLGEELGKRTGRRVLSVDGGFKVEVAFESKGKLLGVDIME
                     IGTYWSQNNPDGSLYGEGQGVVITHDGEVATWKGAGVGKFVGGGAVSYRGAIYYSTAS
                     EKLARLNGGATVFEFDVDPEGNTHSKLWEWK"
     gene            8173..8583
                     /locus_tag="VLE22_25840"
     CDS             8173..8583
                     /locus_tag="VLE22_25840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17898.1"
                     /translation="MKRIALLILTATACLAADDPWAKVRELKRGGELRVYEVNAKEPI
                     RATFDRASDESLIVITKDGQVSILKEDIERVDCRRGERRPLVKDTKVERKIAPKGAEV
                     TSNTIPGATTSVKTRVDLPSKAAFDTIYERKPAK"
     gene            complement(8629..>9667)
                     /locus_tag="VLE22_25845"
     CDS             complement(8629..>9667)
                     /locus_tag="VLE22_25845"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSB17899.1"
                     /translation="LPPEKLREIGLLPETWSLDVVPDPASDSSVEQPLSQALGNALDW
                     NGLMRLAEKHAVRFLHVCVCTNGADPFHMTLWEGVPLREILWLARPKANVRRVYYQSY
                     HPENLLPFQASLPLAQILENPPGQMPVILAYKMNGQVIPASHGGPARVIVPGAYGSKQ
                     IKWIQRVVLTNDYKPNDSDAELNNDTESPMKTRARFINAPAEIPPDKPAALTGMAQVG
                     ISGLAKVQYCVHSQKEPWPADDPHWTKADWKDVAVLPPPADWGGGLPGGKLPALTGQA
                     DPAKGAPLQWPQRYTIVHWAALLPVLPPGSYDLCCRTIDANGIAQPMPRPPLPRTGYN
                     AIHRVTLVVKA"
BASE COUNT         1822 a         2847 c         3060 g         1938 t
ORIGIN      
        1 ctctgcccgg cgatgcgctg attgacggcg gcgtcgctga gcttttgccg gatcactctg
       61 cccgaatcaa gtctggcgcc cgcctcggcc gatgcggaat cggggatcca ggcgagctcc
      121 cgcgcggcga accgggcaga tgccgccttt tcggccgcaa gaacgtagtc ttcataggca
      181 tccagggcaa ggggctccag aaccgtcgta caccgcgctg cccagcacat tccgctgaac
      241 ccgcaggcca gcaccagcaa tctgaaagga gggctcatcg atggagagcg gccactagcc
      301 ggatgaactc gggattagcg gccagcttgc cagcgcgggg ctcgacacgc aggtacatca
      361 ggtaatcaca gcgctcgccg accgccttcc accccaactg aagggccctc gcaacgtcac
      421 ccatcgagta gtagatcgac gcaaggtaga atgccggaac ataccgcgtg cgcgacatct
      481 cttcgaggcg ggccaactcc ttcctggcat ccgcgatctt gccagcggct gcatatgcgc
      541 ttgccagagc cgcgacgggg ccagggctgc cgcccgagag cgccaccgcc tgttggaact
      601 cgtcaatggc ctccgagaac atccctttct gttggtacgc catgcccagc gtctggtgcg
      661 ccaggggaag ccgaggctcc agatcaacca ccgaacgata ctgcccgatc gcctcgtcgt
      721 aacgcctggc gtaataatgg caccacccga tcccgatgtt aatgccgggc gaaagcggtt
      781 caagtcgaag cgcctcacgc atctgttcgg cagccttact caggtcgccg gcggccatgt
      841 agtagtggct gaaccaatga tgcgccgtgg cggagttcgg gttcagcgcc agcgcgcgag
      901 agaactcctt ttcagcagcc tgaaggtccc agtcatacga cagtttgacg taggcaagcg
      961 aggtgtgagc gtcggcgagg ttcggctcca actcgagggc cctctgcgcc gcggtcttgg
     1021 ccaggggcat ggctttcaca ggcggcattc cgtcgacccc gatcgacccc agcaatgcgt
     1081 aggagtccgc caaaccgctg taagcaagcg cataggctgg gtccagcgcg attgcctgct
     1141 ggaagtattg cacggcgcgt ctcaacccgt cctctgtccg gcggttccag aagtgccgcc
     1201 cacgaaggta ggcgtccagt gcttcgggat tgaccgtccg gtttgtgcgg aggttgcgtt
     1261 tctcctctgc ggtgatggtc acctgaatct cgccggcgat ggcttcggcc actgccgcct
     1321 ccagagtcaa aacgtccgtc atgtcccgtt cgtagtagtt cgcccaatat acggctcgtg
     1381 tgccggggtc gatcaactgg gcgtcgattc ggactcgctg ccccgaacgg agcactgtcc
     1441 cagtgaggat ccggttgacc gactgatccc ggaggacttt gctgagagct gctggcttct
     1501 cccgcagagc cgactccgac gctaaggaaa tgacgcggag gctgcccaac ttggccaaag
     1561 ctgtgaccaa agcctgtgtc ataccctcgg caaagtagtc ctgaccgggg cccgtctcca
     1621 gactgcggaa cgagaaaacg gctagcgtgg tgcgaggcgt ccttaccgga gcgcttaagc
     1681 tgcccggccg tgaccgaaac aggtacacgg tcagagctgc gactcctgcc agcactagga
     1741 ggagtgcacc ggccaacaca agccggttcc gtctcatccc ctccggagaa ggctggagtt
     1801 gcaccgcgtc cgccggggtg gccgagagag tctcgcccgc cgccgggctg tccgtttcca
     1861 gggcggcggt gcggaccggc gccacgaacc ggtagccgcg tctatggaag gtttcgatat
     1921 agaccggttc ctgcccgaca tctccgaagg tcttcctcag cagggagaca ttccgagcga
     1981 gggcgccctc ttccacgtag acatcgggcc agacggtctt catcaggtcg cccttgtcga
     2041 ccatccggcc tggattctcg agcagagcca gcagcgtggc aattaccttt ggcggaagcg
     2101 aaaccacttc ctcaccgcgg tgtagcagtc gctcacgcgt gtcaatccgg taagggccaa
     2161 actcgtagca ccgagattgc ggggacgcca tggttcccat ctgccgaaag tcccgttcct
     2221 gtgccagcac gagagactag gcgacgtacg aggccggaac catcgcatca ggattctatc
     2281 agagtttcat cagggtttcc gaaggacgct gacgaggctt gcgtacacac ttgattctgg
     2341 cctgagacga gacgcaaggc caggaaacgg aggtgtttat gaggatggcc atcgtagctg
     2401 ttgcgttgct aatggccggt tgcacagcgt cactccatcc gctttacagc gaggacgacg
     2461 ccgtattcga tccgatgttg ttggggacct ggcgggaatc cgagggtgag aagttctttg
     2521 tgtcagtccg cgccgagaac gcggcctatc gcgttatcta ctacgacggt gagggaagcg
     2581 gcgcgttcaa ggcacggctg gtcgagctac aaggcaaccg gtttgtagac ttataccccg
     2641 agacaccgcc gtctaagaac gggttctaca acgttcatct gattccggct catagctttg
     2701 ggcggatttt gctagccgga gaccgactca caatcaccct gctcgacgcg gactggcttc
     2761 gctccgaaca ggcgagccag gttctactgg agcgaatctc tctggacgat tgccaggtca
     2821 tctcggcggc gacggcggac ttacggcgct tcgcgacaaa gtacgccagt acaaaagcct
     2881 tttccgttac gagcgagtgg gtacgagaac aatgattcgt caggtccacg ggtatgcccg
     2941 gggcaaggcc atatgaacac gacgtgggaa ttcaggacgt gggggcttaa gtgatgcatc
     3001 tccaaaggat tcggcaattc gccggcgttg ctttgatcgc cgccgtgtcg gtgggatgtt
     3061 cgatgaaaag gatggcggtg aacaagatcg gaaatgcctt ggcctccgga ggttccacgt
     3121 acgagagtga cgaggacctc gaactggtcg gagacgcatt gccgttcggg ttgaagctca
     3181 ttgagagcct gctggcggaa tctccgcgtc atcgcgctct cttgcagtcg gcttgccagg
     3241 gcttcacaac ctactcctac ctttacgtgc agcaggaggc ggaccggcta gctgacaacg
     3301 accttgacgc cgctgaccgg ctgcgtgccc gtgcgagacg gctgtttttg cgggcacacc
     3361 gctacggatt ccgcctgctg gagacaacgg cgccaaacgt cagtgctcag atgaccgacg
     3421 atcccaaagg cgctacgctg cgattccgga aacgaaccga agtgccgtcg ttgtactgga
     3481 acgccgctgc tctcggcctc gcgatcgcgg tatccaagcg cgacgcggcg atgctcgccc
     3541 ggctgcccga ggtggaagcg ctgatcggcc gggcggtgga gctcgacgac acgtggggcg
     3601 acggtgcgct ccacgagttt gaagtaatct tggccggagc caagccgggc aagccggatt
     3661 ttgatgccat cgaagtccac taccagcgtg ccctgacact atccaagggc aagcgggcca
     3721 gtgttcacgt ggcctatgcc gaatccgttt cggtgccaag acaggaccgc gaaggtttcc
     3781 gcgcctccct ggaaaaagcg ctggccgtgg acccggacca gtatcccgag gtaaggattc
     3841 ccaatctcgc cgcccagcgc cgagccagat ggctgctgga gcgcgtggat gagctgatac
     3901 tgccgcccgt tgcggcactt gaggaggaga aatgaaaacg aaattcttct tggtgattct
     3961 gttcgcctgg tccgccttat tcgcacaaaa gcttcaagtc aacgtcggca ccgccgcacc
     4021 cgagggatca ccgtggcacg agattctaca gaagacccgc caagactgga accgaatctc
     4081 gggcggcaac ctcaccgtgc gtatttatcc cagcggcgtg ctgggcgacg aggtcgagat
     4141 gatccggaag gcgcgcatcg gccagttgca ggccgtcgca gtctcggggg ttgggctgtc
     4201 gcacattgag ccggcagcgg gctgcctcca gatcccgatg ctgatgggtt cgtatgagga
     4261 attcgactac gtgcgggaac gaattgcacc gcggttggag tccctcatcg cgcaaaaagg
     4321 cttcatcgtg ctgcaatgga gcgacgtcgg ctgggttcat ttcttcacca agaagcccgc
     4381 gaagacgccg gacgacatcc gcaagctgcg gctgttcacc tcggcggggg atccggaggg
     4441 cgagaggctg tacaaagagt ttggtcttca ggttgtcccg ctggccgtga cggacatgct
     4501 gccgtctctg cagacaaacc tgatccaggc tttcgatgtt cctccgttgt tcgcgatgtt
     4561 ggaccagtct ttcggtctgg cgccgaacat gatcgacgtg aagtggacgc cgctcgcggg
     4621 cgcgacgctg ctcagccgaa agacgtggga ccgaatcccc gccgaacagc ggacggagtt
     4681 gctgcgggcg tcgcgatccg cgtccgcggg cgtccgcgcg caggtccgca agatgggaga
     4741 ggatgcgatt gtcgaaatgc agaagcgcgg gctcaacgtc atcaagctcg atcccgccac
     4801 actctccaaa tggcaggtag aggcgaagtc atcgtatccg aagattcgcg gggaagtggt
     4861 accggccgac ctgttcgacg aggttgtgcg actgcacaat gagtacctct cgaggatggg
     4921 aggcaagggg taatggaaac ttgccggact ccaacgacgc ggattgaagc atgtgcgtcg
     4981 attgcccttc tcgggttgat ggccgcacta ccgctcgcgg agattgcggg ccgcaagatc
     5041 gcgggccacg gaattccggg gtccatccca atcgtccagc acctcacctt atggattacg
     5101 tttcttggag cggcgatggc ggtgcgatcg gatcgtatgc tggccctctc cacggccacg
     5161 ttcgtttctg aaacatggcg tgcgcgcata cgagtcctga cgtccaccgt cgccgtagcc
     5221 attaccctct gtctggccgc ggccagtctc gagcttgcgc gggtggagcg agaggcgggt
     5281 gatctcgtcg catggggtat cccggtctgg attgcgattg cgatcatgcc cgccggattc
     5341 gcgctgatcg gattccggct catctggagt gccgcgccac actggaaagg ccgcgcggcg
     5401 gcggcaaccg ggctggcaat tccgttgttg ttccacagcg tctccgcttc ccggagcgcc
     5461 gatctgacgg tgggcgccgt gattgcgatc ctcggaggcg ccgcacttgg gatgccaatt
     5521 tttgccgtgc tgggcgggct ggcgcttctc ctgttttgga atgccggtgt atcggcagcg
     5581 gccgtcacgg cggacatcta ccggctggca gcctcaccga tgctcccggc gatcccgttg
     5641 ttcacgctcg gcgggtatct gctctcggag ggaggcgccg gcaaacggct catgcggacc
     5701 ttcaccgcat tggtgggctg gatgccgggc ggactggcgg tcgttactac tcttctgttg
     5761 gccttcttca caccgtttac cggcgcatct ggagtgacga tcctgtcgat gggcgggttg
     5821 ctgttgccga tgctggttaa ggcgcgatac ccggagggga cttccatcgg cctggtgacc
     5881 gttgccggct ctatcgggtt gttgttgccg ccgagtttgc ccgtgatcct ctatggaatc
     5941 tacgcgcaga caccgatcga ccggctcttt gtcgccgggc tgatacccgg cttcctgctg
     6001 atcgtggcag tggccggatg gggcgcttgg cgtggatggt tcagcggtgc tcaacgtgtt
     6061 cgtttcgacg cgcgagaggc cgccgcggca acatgggatg ccaaatggga actgctgctg
     6121 cccgtggtag tgctggctgg gatgctcggc ggattcgcca cgctggtgga agcggcatct
     6181 ctgacggtgc tttacgcgct catcgttgaa tgccttgttt acagggacct ccgcgtgcgc
     6241 tccgacctgc cccgcatcgc ctctgagtgc gggacgctag tgggcgggtt cctgatcatc
     6301 cttggcgtcg ccctcggctt taccaatttc ttgattcacg cggaaatccc gatgcaggcg
     6361 ctcacgtggg tcaagttaca cattcactcc aaacttgtgt tccttctggc actcaatggc
     6421 ttacttctcg ttgtaggcgc attgatggat atctactcgg ccattctggt ggtggtcccg
     6481 ctgatcacgc caatggcggc ggcatacggg gtccatccgc tgcacctggg cgtcattttc
     6541 ctcgccaaca tggagcttgg ctacctgacc ccgcccatgg gggagaatct ctttctttct
     6601 tcgtttcgct tcgggcggcc actcagccag gtttacgcgg acacactgcc ctattgcgcg
     6661 atgctcctgg cgacggtgct cctgatcacc tatgcgccat ggatcaccat gctgcccatt
     6721 tctttcctag agggtggatg aacttcacga aactagccgt gattcttgtc ttgggagcca
     6781 tgacgccacg cgccttctca cagagcagct tcgagatcca gccgttcgcc ggatttcgat
     6841 tcggcggcac gattcggacg gcggacgatg atcccctgcc catcaaggtc gggagcagtc
     6901 cggttgcggg cgcgacattc acgtacggtt tcgacgagtc cttcggactc gaattcctgt
     6961 gggcccacca gagtaccaga gggatgaccg ctgtccagaa ggccaatctt cggctggacc
     7021 agttcctcgt ccatgtggtt cttaacatgc gtgagcgcga gaagagcctt caacctttcc
     7081 tgcttggcgg cgccggagcg acccacgcga gtggtgcaac ttcaatcagc acgaaggtcg
     7141 cgtggagtgt tggtggtggg atcaagtact tcgtctccag acacttcggg tttcgattgc
     7201 aggcacgata cgcgccaacg gaactctatt cgacgccctt acctgactcc gactcctact
     7261 gttcgggtat cacgtattgt ggtgacccga agaagcaaca cgacctcagc cagggagagg
     7321 tgacagcagg atggattttc cgtttccctt agtgtcctga cacccaagga tccgacgcgt
     7381 gtttccgcga agttctacgg ctggtcgcgg accacggttt cgtgagccgc gatcagccac
     7441 ctgccgttga tctttctgag cacgatcagg ttacggccgt cgacccgacg gcaagcatga
     7501 tgagtgcgcg tttcatgcgg gcccttgata ctgtatagcc agtgaaaatc aggattccag
     7561 gatattctaa acccatatct ccatttcgaa ctccgcctgc ggttctaaag gtgaacgggt
     7621 gtatactctg agtaacccgg ggccaaaatg caaatcccgg gagaggggac aattcatgct
     7681 cggagaacaa ctgggtgaag aattaggcaa gcggaccgga aggcgtgtgt tgtcagttga
     7741 cggcggcttc aaggtggagg tcgcgttcga atcgaaggga aaactgctgg gcgtcgacat
     7801 catggagatc gggacatact ggtctcaaaa caatcccgac ggcagcttat atggcgaagg
     7861 ccaaggcgtg gtgattaccc acgacggcga agtagccact tggaaaggtg cgggagtggg
     7921 caagttcgtg ggtggtggcg cagtcagtta ccgcggtgcg atttattaca gcaccgcctc
     7981 ggagaaactg gcccggctga acgggggtgc aactgttttt gagttcgatg tcgaccccga
     8041 gggaaatacg cactccaagc tctgggagtg gaagtagttt cagtcgggat atcttccagt
     8101 cgtgtcaggg ataccgggcg cccggcactc cctgtgctgt ccaagacgtg gagttgtgtc
     8161 gtgcagcgca ttatgaagcg aatcgctctt ctgatcctga ccgcaacggc atgcctggcg
     8221 gccgacgacc cctgggcaaa ggtgcgggaa ctgaaacgcg gcggcgaact gcgcgtctac
     8281 gaggtgaacg ccaaggagcc gattcgtgcg acgttcgacc gagccagcga tgagagcctg
     8341 atcgtcatca cgaaggacgg gcaggtttcg attctgaaag aggatatcga gcgagtggac
     8401 tgccggcgtg gggagcggcg cccgttggtg aaggatacga aggtcgaacg aaaaatcgcc
     8461 cccaaaggag ccgaagtcac gagcaatacg attcctgggg cgacgacgtc ggtgaaaacc
     8521 cgcgtcgacc tcccgtcgaa ggcggccttt gacacgatct acgagcgtaa gcccgccaag
     8581 taagtagccc ctagactgat tgctatcgcg ggcggcccgg aaactgggtc acgctttcac
     8641 gaccagggtg acgcggtgga tggcgttata gccggtcctc ggcagtgggg ggcgcggcat
     8701 gggctgcgcg atgccgttgg cgtctatggt acggcagcac aggtcgtaac ttcccggagg
     8761 cagtacagga agcagcgccg cccagtggac aatcgtgtaa cgctggggcc attggagggg
     8821 cgcacccttt gccggatcgg cctggcctgt gagggcgggc aacttcccgc ccgggagccc
     8881 tccaccccag tccgcgggcg gcggcagaac tgcgacgtcc ttccaatcgg ccttggtcca
     8941 gtgcgggtcg tctgccggcc acggttcctt ttgagaatgc acgcagtact ggactttggc
     9001 gagcccggat atgccaacct gcgccatgcc cgttaatgca gccggcttat ccggcggaat
     9061 ttccgcggga gcgttgataa acctggcccg agtcttcatc gggctctcgg tgtcgttgtt
     9121 caactccgcg tccgaatcgt tgggcttgta atcgttggtc aggacgacgc gctggatcca
     9181 cttgatctgc ttgctgccgt aagcgcccgg aacgattacc cgcgccgggc cgccatggga
     9241 cgccggaatg acttggccgt tcattttgta ggcgagaata acgggcatct gccccggcgg
     9301 gttttccaga atctgagcca gcggcaacga agcctgaaaa ggcagcaggt tctcggggtg
     9361 gtagctctga tagtagaccc ggcggacatt ggctttgggc ctggccagcc agaggatctc
     9421 gcggaggggc acaccctccc acagggtcat gtgaaacggg tcggcgccgt tcgtacagac
     9481 gcagacgtgg aggaagcgga ccgcgtgttt ctccgccaat cgcatcaggc cattccagtc
     9541 cagggcgttt ccgagcgcct gcgagagggg ctgctccacg ctgctgtcgc tggcagggtc
     9601 cgggaccaca tcgagagacc aggtttccgg aaggagtccg atctcgcgca gcttctccgg
     9661 cgggagt
//
LOCUS       DATIFQ010000512         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2474730, whole genome shotgun sequence.
ACCESSION   DATIFQ010000512 DATIFQ010000000
VERSION     DATIFQ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474730"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(473..1897)
                     /gene="melA"
                     /locus_tag="VLE22_25850"
     CDS             complement(473..1897)
                     /gene="melA"
                     /locus_tag="VLE22_25850"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HSB17900.1"
                     /translation="MSVKVAMIGAGSIVFCKTLLSDMMATPALAGAEFALMSRTEPKL
                     RRMEEFARRMLKDNGVPGSVWATLDRREAIRDADFVVVMIQVGGVDAFEIDYKIPLKY
                     GVDQCIADSMGPGGIFRGARTIPVLLEIARDMEETAKPGAIMLQYANPMAANCLALGK
                     ASRTAFVGLCHGVQTTLDLIAGYCGVPKEEITYRCGGINHMDWFLSLEHKGRDLYPTL
                     RELFEKPEYYKNEKVRGEVFRHFGYFMTESTGHLSEYVPWFRKNRKALDLYCDEPSFG
                     GESGAYYNWCRTVATKYAESDALQYESTLIEERSVEYCSHVLEAVVTGKPFRFMGNVR
                     NDGYITNLPRGCCVEVPAFADDTGLHPTFVGELPPQCAGLCMTNINSQILAAEAAIDS
                     DPERLMQAVAMDPLTAAVCTLGEVREMCTEMLEAQRPWLPGFDGKRIAPKLVIAIPPD
                     CQPVAVPLDPALAIGKRFGTLVEG"
     gene            complement(1930..>2782)
                     /locus_tag="VLE22_25855"
     CDS             complement(1930..>2782)
                     /locus_tag="VLE22_25855"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HSB17901.1"
                     /translation="YVGAYEHEEIDAKKIAEWGFDLLKYDWCSYGKIAREKTLEEFQK
                     PYRKMGAILQGLDRDIQLNLCQYGMGDVWKWGGEVGGHSWRTTGDLGLEKDTRLPGFY
                     QIGMRNAEHSEYAGPGRWNDPDYILIGVIGNAHRIDEAPKPTNLTPDEQYSYMSMWSL
                     MASPLFFSGDMGRLDEFTLNVLCNAEVIDVNQDALGKQARIVRRTGEEFVLAKPLEDG
                     SVAVGLFNIGEAPRKLTVKWTELNLSGRQKARDVWRQKDIGVFADSFVADVPRHGVAM
                     MRLSAAK"
BASE COUNT          465 a         1038 c          740 g          539 t
ORIGIN      
        1 aacgcgcgct gtgcgccctc gggccaacgg gaaccatcga ccgcgagagg aaggtagttg
       61 ctacgtttgc gggtagattt gtgtggcaac ctcacctcca attgggttct cgggctctcg
      121 aggccgaaag tattgaacgc atggccatca cacctcctct cgccatgcca tgaggggctt
      181 cgcctgtgat gccgcctgtc gcctccccca aagactgcga gccgacggag aagcgatgtg
      241 cgccctcaca ccggcagggc cgacgcgagg ccggccatgc ctcgctcgct gagcgagacg
      301 gctatgaggg tgagggcgtg cttgtgaatt tgggacactc ggctttcgtt gatgccgtca
      361 gcggaggtcg atctcacgca tcgcgagggg agcgccagtc accccaagat tcgcgttgca
      421 ttcgcgtcta ttcgcggctc acgcctttgc agctgcggct tccgatgccc ccttacccct
      481 ccaccaacgt cccgaagcgt ttcccgatcg ccagcgcggg gtcgaggggg accgctaccg
      541 gctggcagtc cggcggaatc gcgatcacga gctttggagc gatccgcttc ccgtcgaatc
      601 ccggcagcca cgggcgctgt gcctccagca tctccgtgca catctcccgc acttccccga
      661 gcgtgcacac tgccgccgtc agcgggtcca tcgccaccgc ctgcatcagc cgctccggat
      721 cggaatcgat cgccgcctcc gccgcaagga tctgcgagtt gatgttcgtc atgcacagcc
      781 ccgcgcactg cggcggcagc tcgcccacga acgtcggatg cagccccgtg tcgtccgcga
      841 acgccggcac ctccacgcag cacccccgcg gcaggttcgt gatgtacccg tcgttccgga
      901 cgttccccat gaaccggaac ggcttccccg tcaccaccgc ctccagaacg tgcgagcagt
      961 actccacgct tcgctcctca atcagcgtcg actcgtactg aagcgcgtcg ctctcggcgt
     1021 acttcgtcgc cacagtccgg caccagttgt aatacgcgcc gctctcaccc ccgaaactcg
     1081 gctcgtcgca gtaaagatcc agcgccttcc ggttcttccg gaaccacggc acatactcac
     1141 tcagatgccc cgtgctctcc gtcatgaagt agccgaagtg ccggaacacc tcgccccgga
     1201 ctttctcgtt cttgtaatac tccggtttct cgaataactc ccgcagcgtc ggatagaggt
     1261 cgcgcccctt gtgctccagc gacagaaacc aatccatgtg gttgatgccg ccacaccggt
     1321 aggtgatctc ctccttcggc acgccgcagt atcccgcgat cagatcgagc gtcgtctgaa
     1381 ccccgtggca cagcccgacg aacgccgtcc gcgacgcctt cccgagcgcc aggcagttcg
     1441 ccgccatcgg gttcgcgtat tgcagcatga tcgcgccggg cttcgccgtc tcctccatgt
     1501 cccgcgcgat ctccagcagc accgggatcg tccgcgcgcc gcggaaaatc ccgcccgggc
     1561 ccatcgaatc ggcgatgcac tggtccaccc cgtatttcag cgggatcttg tagtcgatct
     1621 cgaacgcatc cacaccgccg acctggatca ttaccaccac gaagtccgcg tcccggatcg
     1681 cctcccgccg gtccagcgtc gcccagacac tccccggcac gccgttgtcc ttcagcatcc
     1741 gccgcgcgaa ctcctccatc cggcggagct tcggctccgt acggctcatc agggcaaact
     1801 cggcgcccgc cagcgccgga gtcgccatca tgtcgctcag cagcgtcttg cagaagacga
     1861 tgctccccgc gccgatcatc gcgactttca cgctcatcgt tgtgcctctc tttctatgtc
     1921 cgcttcccct cacttcgccg cggacagccg catcatcgct acgccgtgcc gcgggacgtc
     1981 cgccacgaac gaatccgcaa acacgccgat atccttctgc cgccacacgt cgcgcgcctt
     2041 ctggcgcccg ctcaggttca gctccgtcca cttcaccgtc agcttccgcg gagcctcacc
     2101 gatgttaaac agccccacgg cgaccgaacc gtcctccagc ggctttgcca gcacaaactc
     2161 ctcgcccgtt cgccgcacga tccgcgcctg cttgcccagc gcgtcctggt ttacgtcgat
     2221 cacctccgcg ttacacagaa cgttcagcgt gaactcgtcc agcctgccca tgtcgcccga
     2281 aaagaacagc ggcgacgcca tcagcgacca catcgacata tagctgtact gctcgtccgg
     2341 cgtcagattt gtgggctttg gcgcttcgtc gatacggtgt gcgttcccga tcaccccgat
     2401 cagaatgtaa tccggatcgt tccaccgtcc cggtcccgcg tactcagagt gttccgcgtt
     2461 gcgcattccg atctgataga agcccggcag gcgcgtgtct ttctcaagcc ctaggtcgcc
     2521 cgtcgtcctc cacgaatgtc cgccgacctc gccgccccac ttccacacgt cgcccatgcc
     2581 gtactggcac aggttcagtt ggatgtcccg gtcgagtccc tgaagaatcg cgcccatctt
     2641 gcggtacggt ttctgaaact cctcgagcgt cttctcgcgg gcgatcttcc cgtacgagca
     2701 ccagtcgtac ttgagcaggt cgaagcccca ctccgcaatc ttcttcgcgt cgatctcctc
     2761 gtgctcgtac gcgcccacgt aa
//
LOCUS       DATIFQ010000513         4556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1901743, whole genome shotgun sequence.
ACCESSION   DATIFQ010000513 DATIFQ010000000
VERSION     DATIFQ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901743"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VLE22_25860"
     CDS             <1..401
                     /locus_tag="VLE22_25860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSB17902.1"
                     /translation="RASDVPLRDGPILVAEDDEAVRKLVSSVLRANNYVVLEAKDGAE
                     ALQIASRNVNSIALVIAVPALPLLSGSRLVERLWILRPDIGVLYISADGDSTGLGAGG
                     AGVKFLQKPFSVTALLFTVDAMLGRRPQRA"
     gene            635..1576
                     /locus_tag="VLE22_25865"
     CDS             635..1576
                     /locus_tag="VLE22_25865"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB17903.1"
                     /translation="MWTSDKIHRTFKASAISALAVLLAAVPLRARTPDNDEKPRSVDV
                     TVQGVAPGLLKQDNFRLLVDNKPVAFQLVPPETRTSILFLVEDSQYSRWMLSKDLATA
                     ILGLLDASPKGTWFGLATYSDRLKLDDDLTQNKERIVSALRNLPQPVWPEVETFDAVY
                     KALDELELIPGRRALVVIGSGYDRSSEHTADEIQSKIEESHTLVYSIGIGSRLRGDYE
                     PYLRYDRRMRFAQDELFLRLIAQDSGGMSWFPKNEDAFRDAARQLELALKNQYRLVYF
                     AKVTGRHEFHPIRIEALEGTGSQRREIDVRARKGWRY"
     gene            1640..2791
                     /locus_tag="VLE22_25870"
     CDS             1640..2791
                     /locus_tag="VLE22_25870"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSB17904.1"
                     /translation="MSRRVTSIGRWAAMFSPICALAFGGLLGSMASAKAGTPVPKWVS
                     TDRPAVSGRVSLEAGFAPVVKRVVPAVVNISSARVVRTSEGANPYSIDPFFRQFFGDR
                     FFRNVPRERLERSLGSGVIVSSDGYILTNNHVVDDATQITITLADKREFKARVIGKDA
                     RSDVAVLKLDIKDLPFVAFGDSSKVQVGDLALAVGDPFGIGQTVTMGIISATGRGGLG
                     IEDYEDFIQTDAAINPGNSGGALVNVEGELIGVNTAILSRGSGGNQGIGFSIPVNMAR
                     TVMGQIMKHGKVVRAQLGVLTQQVSPSIAKAFGLSKPAGALVSDVLPNSPAALSGLKR
                     GDVITAVNGVVIDDSNQLRLKVSMMTPGTTVRLVESSPQRNRTRDPCHA"
     gene            2742..3128
                     /locus_tag="VLE22_25875"
     CDS             2742..3128
                     /locus_tag="VLE22_25875"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSB17905.1"
                     /translation="MKVLRSGTELEIPVTLSELRSQAESREGGSRGSGNALEGISVDA
                     LTPDIASELRLSPGTKGVVVTQIDPSSSSAEAGLRRGDVIQEVNRKPVANVAEFEAAV
                     RQAGRQPVLLLVNREGNTFYVVAGAQ"
     gene            3137..3961
                     /locus_tag="VLE22_25880"
     CDS             3137..3961
                     /locus_tag="VLE22_25880"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSB17906.1"
                     /translation="MFTSIVWAGGLFAGFLGALTGLGGGVVIVPLLTLALGVDIRYAI
                     GASLISVIATSSGAAAAYVKEGFTNLRIGMFLEMATTFGAILGAFLAGWISTGALAVI
                     FGLVLLHSAWLSSRPRRDRSGDGAPDSITARLKMDGSYPTPEGPRFYRVHSVPAGFGW
                     MFVAGGLSGLLGIGSGAVKVLAMDQAMRIPFKVSTTTSNFMIGVTAAASAGVYLNRGY
                     IDPGVAAPVMLGVLIGSLAGARFLAGARTALLRKTFGVVVAALAIEMIYNGLKGRI"
     gene            3962..4351
                     /locus_tag="VLE22_25885"
     CDS             3962..4351
                     /locus_tag="VLE22_25885"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HSB17907.1"
                     /translation="MSRTEQSWKNDRVERAIGNLLRFGVLLSGAVVLAGLVIYLARYG
                     AQTPHYRVFRGEPADLRSVGAVIRGGSTLGGRELIQFGLLLLMATPVARVVFSIVAFA
                     LERDGTYVAVTFVVLSALIYSLAGGYR"
BASE COUNT          853 a         1316 c         1457 g          930 t
ORIGIN      
        1 ctcgagcctc tgacgttcct ctgcgcgacg gcccgattct cgtcgccgag gacgatgagg
       61 ccgtccgaaa actcgtgagt tccgtcctgc gagcgaacaa ctacgtcgtc ttggaggcca
      121 aagacggtgc cgaggcgctc cagatcgcct cacggaacgt gaactcgatc gccctcgtga
      181 tcgccgtccc ggctctgccg ctactcagcg gctctcgctt ggtggagcgc ctgtggattc
      241 ttcgtccgga catcggagtc ctctacatct cggccgacgg cgattccacc ggacttggag
      301 cggggggggc cggagtcaaa ttcctccaaa agccttttag cgtgacagcc ttattattca
      361 cagttgatgc gatgctcgga cggcggcccc agcgagcctg atcgagcctt gcttgccgac
      421 tatctgttcg tctgaccgtt ccagtgggct gcgtcttcac cacaccggag cagcggtttc
      481 tcccgttgtt tcatcgcaag aactacttgt cggctggtat tcatcgacga acatcgacac
      541 cgtactctgg aagtgtggca cgagtccgca gcgggtggat cgaaccacga tgaggccgac
      601 agtgagtcta caaaaaggag gaaattgaca cgttatgtgg acatcggaca agattcatcg
      661 cactttcaag gcaagcgcaa tctcggccct ggccgtcttg ctcgccgccg ttccgttgag
      721 ggcgcggact ccagacaatg atgagaagcc gcgatccgtg gatgttaccg tgcaaggggt
      781 ggctccgggc ctgttgaagc aggataactt ccgtttgctt gtggataaca agccggtggc
      841 gttccagttg gtgccgcccg agacgcggac ttcgattttg ttcttggtgg aggacagtca
      901 atactcgagg tggatgctct ccaaagacct ggcgacggcc attctcggtt tgttggatgc
      961 ttccccgaaa ggtacttggt tcgggctggc cacttactcg gaccgtctga agttggatga
     1021 cgatctgacg cagaacaaag agcggatcgt ctccgcgctg cggaacctgc cgcagccggt
     1081 atggccggag gtggaaacct tcgatgcagt ttacaaagcg ctcgacgaat tggaactgat
     1141 cccaggccgg agggccctgg tggtgattgg atccggctac gaccgatcca gcgagcacac
     1201 tgccgatgaa attcagtcga agatcgagga atcccacaca ctggtctact cgattgggat
     1261 cggctccagg ctgcggggcg actacgaacc ctatctgagg tatgaccgcc ggatgcgttt
     1321 cgcgcaggac gagctcttcc ttcgcctcat cgcacaggat agcggcggca tgagttggtt
     1381 cccgaagaac gaagatgcgt tccgggacgc ggccaggcaa ctggagcttg ctcttaagaa
     1441 ccagtaccgg ctcgtgtact tcgccaaagt cacgggaaga cacgagtttc atccgatccg
     1501 gatagaagct ctcgaaggaa ccggcagcca acgccgcgag atcgatgtcc gcgcacgaaa
     1561 ggggtggcgg tactgacgcc cagcggcgtc gtatggcagt cgtgtgtatg acaccaaacg
     1621 atggaaggag ttacaaacga tgagcagacg agtcacttca atcggccgct gggcggcgat
     1681 gttctcaccg atctgtgcgc tggccttcgg gggcctattg ggctcgatgg ccagcgccaa
     1741 agccgggacg ccggtcccca aatgggtttc tacggaccgc ccggccgttt ccggccgcgt
     1801 gtcgcttgag gccgggtttg cgccggtggt caagcgcgtc gtgccggccg tcgtaaacat
     1861 ctcttcggcg agggtcgttc gcacgtcgga aggagcgaat ccgtattcca ttgacccgtt
     1921 cttccggcag ttctttggag accgattctt ccgtaacgtg ccccgtgagc ggctggagag
     1981 gagtctgggt tcgggggtca ttgtcagctc cgacgggtat atactcacaa acaaccatgt
     2041 tgtggacgat gcaacccaga taacgatcac gctcgccgac aagcgcgagt tcaaagcgcg
     2101 tgtcattggc aaggacgcgc gatcggacgt ggccgtcctg aagctggaca taaaggatct
     2161 gccgttcgtg gcgtttggag actcctcgaa agtccaggtc ggcgatctcg cgctggcggt
     2221 gggggacccg tttggaatcg ggcagacggt cacgatggga atcattagcg ctacaggccg
     2281 gggcgggctc ggcatcgaag actacgagga cttcatccag accgacgcgg ctatcaatcc
     2341 cggcaactcg ggaggagcgc tggtcaacgt cgagggcgag ctgatcgggg tcaacacggc
     2401 cattctttcg cgtggctccg ggggaaacca gggaatcgga ttctccatcc cggtcaacat
     2461 ggcccgcacg gtcatggggc agatcatgaa acacggcaag gtggtccgcg cccagctcgg
     2521 cgttctcacg caacaggtct ccccgagcat cgccaaagcc ttcggcctta gcaagcccgc
     2581 cggcgccctt gtgtcagacg tactgcccaa tagcccggcg gcgctcagtg gcctcaaaag
     2641 aggcgacgtc atcacagcgg taaacggcgt ggtgatcgac gacagcaacc agttgcggct
     2701 caaagtctcg atgatgacgc ctggaacgac cgtcaggttg gttgaaagtt ctccgcagcg
     2761 gaaccgaact cgagatccct gtcacgctta gcgagttgag gagccaggcg gaaagccgcg
     2821 aaggcggaag ccgcggttca ggcaacgcgc tggaaggtat cagcgtggac gcgttgacgc
     2881 cggatatcgc gagcgaactg cggctatctc ccggaaccaa gggggtggtc gtcacgcaga
     2941 tcgacccgtc cagctcgtct gcggaagcgg gccttcgtcg cggtgatgtg atccaggaag
     3001 tgaaccgcaa gccagtcgcg aacgtcgccg agttcgaggc ggcggtgcgc caggccggca
     3061 gacagcccgt gcttcttctg gtcaaccgcg aagggaacac cttctacgtt gtggcgggcg
     3121 cgcagtgaat acgtggatgt tcacttccat cgtctgggcc ggcggtctgt tcgcgggctt
     3181 cttgggggcg ctcacaggcc tcggaggtgg agtggtgatt gtgcccctcc tgaccctggc
     3241 gttgggggtg gacatccggt acgctatcgg cgcgtcgctg atctcggtga tcgccacttc
     3301 ctctggagca gccgccgcgt atgtcaaaga aggcttcacc aacctgcgca tcggaatgtt
     3361 cctggaaatg gcgaccacct tcggcgccat cctcggcgca ttcctggccg gatggatttc
     3421 cactggcgcg ttggccgtga tctttggtct tgttcttctg cactccgcct ggctgtcgag
     3481 ccggccccgc cgggaccgtt ccggcgacgg cgcgcccgat tcgatcaccg cgaggctcaa
     3541 gatggacggc agttacccaa cccccgaggg accccggttc taccgcgtgc actccgttcc
     3601 ggcgggattc gggtggatgt ttgtcgccgg tggcctctct ggtttgctcg gaatcggatc
     3661 gggcgccgtc aaggtgcttg ccatggacca ggcgatgcgc atcccattca aggtttccac
     3721 cacaacaagt aacttcatga tcggcgttac tgccgccgcc agcgccgggg tctatctgaa
     3781 cagaggttac atcgatcccg gtgtggcggc accggtgatg ctcggcgttc tcattggttc
     3841 gctggccggc gcccgtttcc tggcaggagc aagaacagcc ctcctgcgca agacgttcgg
     3901 cgtggtggtg gccgcgcttg ccattgagat gatctacaac ggcctgaagg gaaggatttg
     3961 aatgtcgcga acggagcaga gctggaagaa cgatcgcgtt gagcgtgcga tcggcaatct
     4021 gcttcgcttc ggcgtccttt tgtcgggggc cgtggttctg gcgggactcg tcatctatct
     4081 ggcgcggtac ggggcccaga cgccacacta ccgcgttttc cgtggcgagc cggccgactt
     4141 acggagcgtc ggcgccgtaa ttcgaggggg ttcgacgtta gggggccgtg aactgattca
     4201 gttcggattg ctcctgctga tggcaacgcc tgtggcgcgt gtggtcttct ccatcgttgc
     4261 atttgcgctc gaacgagatg gcacttatgt ggccgtcacc ttcgtagttc tttccgcgtt
     4321 gatctacagt ttggccggag gataccggta agcgtgggct gtcttctcaa gggcgccgac
     4381 gtatacggcg gactatggca atacgggtag cgccgccgga gaccagggga gggtatcgcc
     4441 gaaggctaca atggaaagct ggccatcatg attcagactc tttttggttc cgcgccgaag
     4501 gaacccaatc tgctcgatcg cctgaagatg ggtgtccaga agacacgcgc gggcct
//
LOCUS       DATIFQ010000514        16146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1135469, whole genome shotgun sequence.
ACCESSION   DATIFQ010000514 DATIFQ010000000
VERSION     DATIFQ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 16146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16146
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135469"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VLE22_25890"
     CDS             complement(<1..887)
                     /locus_tag="VLE22_25890"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HSB17908.1"
                     /translation="MIRTIPSVLFAFFAFVLGFQPALAADPAYLWSKAFGSPTNDQGT
                     AVATDASGNVYLTGSFTGSANIGGQNLVCGTSGDCLFLAKFAPDGTPLWSKSFNSGYA
                     SGKAVATDADGNVFLAGVFANANFGCGTVSGSIFAAKLAPDGSCLWSKPFKSVATGGS
                     TNAGGNDAASGLAIDAGGNVFLTGKFGCSTSEGCAINFGGGLITSPQRPGYGGIFLAK
                     LAGTDGSHQWSKAYGGVSGMDYTSGVAVDSSGNPSITGYFSSSILNLGGSNLTNAGPP
                     DGFLAKYRGSDGAPLWSKR"
     gene            complement(1506..2345)
                     /locus_tag="VLE22_25895"
     CDS             complement(1506..2345)
                     /locus_tag="VLE22_25895"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSB17909.1"
                     /translation="MTNSFILGMGQMLVEGGKPARNLQRARAMILCAAEQGCRIAVLP
                     ECLDLCWTHPSARRKAQPIPGPHSEAIAAAAREAGIHVAAGLVERDGDRIHNAAILVS
                     PSGEILLKHRKINELTIAHSLYTPGGRLEVVPTPLGVLGITICADNFPDSLALAHSLA
                     RMGCRLLLSPSAWAVDADHDNEKKPYGALWMKSYTTLAKLYDLTVIGVSNVGWLTGGP
                     WKGRKCIGCSLAVGPGGNVLSQGPYGEKAEALIPVPVEIKPARASGTAIADLLRVRGY
                     TGP"
     gene            complement(2356..3732)
                     /locus_tag="VLE22_25900"
     CDS             complement(2356..3732)
                     /locus_tag="VLE22_25900"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HSB17910.1"
                     /translation="MTKPATEMVPKDLYELRPEDVADPPQTLFAILRRIGPGMILAAS
                     IVGSGELIATTTLGAQAGYAALWVILLSCFIKPAVQAELGRYTIATGETGLQGFDRVP
                     GPRWKVRWVVWAWAFMVLMTLFQVGAMFGGVSQVMNQIIPAVPVNAWVFLFLVITLAL
                     LLGGGYERIEKLAMIKVGLFTMLTFLCALLLIRMPQFFSWDRLLSGLTFQMPGEGLTT
                     AVAVFGITGVGATEMFMYPYWCVEKGYARCAGRNDGSPMWRWRALGWIRVMHVDIFAS
                     MLIYAVATVAFYLLGAGILNGMGLVPAAKDMIPVLSNIYTQTLGTWSLWLFYVGAIAT
                     LYGTIFAATAAHSRVYADMCRLMGLFPAHDYAMRLRWRSRFVWLLTVLPVGLFFIFQS
                     PVKMVIAGGVAQALMLPVIAIGALYLRYRHLPKEVIPRRWVTLGLWFASGVIVCLMAY
                     YAVLTIRS"
     gene            complement(3775..4170)
                     /locus_tag="VLE22_25905"
     CDS             complement(3775..4170)
                     /locus_tag="VLE22_25905"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB17911.1"
                     /translation="MVLDASAILAVLLQEPGHEPILNRIGEAPVVAVGVPTLVESAIV
                     LSSRLRRDARGLLTDFLREADAEVVPFTLDHYYVAVDAFLRYGKGRHPAALNFGDCLS
                     YATARLSGLPLLFTGDDFSKTDIATAPPS"
     gene            complement(4174..4446)
                     /locus_tag="VLE22_25910"
     CDS             complement(4174..4446)
                     /locus_tag="VLE22_25910"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HSB17912.1"
                     /translation="MALNIKNSEVESLAAEVARLAKETKTEAIRRALLDRKLRLQARR
                     GNISRKARMEALLREQIWPQIPARLRGTRLSRAEREQILGYGSEGV"
     gene            complement(4477..5811)
                     /locus_tag="VLE22_25915"
     CDS             complement(4477..5811)
                     /locus_tag="VLE22_25915"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSB17913.1"
                     /translation="MRSLVLLLCTAGVVLAGSLPFRQSGFNAGPDGTPEGWTAWSPRA
                     EIAPRTFVDAVHSRGQQGSLAISGNSNPAEFGGWQYTASGVQPGKWYRLVAHYRTEGQ
                     IYQPLHLWARLDWAAADGRRAGQPEYGYATTPVEGWNRITTQAPAPEKATAVRVQLFL
                     LNSPQATVWWDDISLEEVPAPPPRNVTVASINFRPSGSKSAAENVRRFVEVVDKTAPA
                     KVDVILLPEGITIVGTGKTYVDVAEPVPGPTTERLGEAARRHNAYVAAGIYEREGSAV
                     YNTAVLIDRAGKVVGRYRKVYIPREELEGGITPGLDYPVFETDFGRVGMMICWDLQFA
                     DPSRALALRGAELLLLPIWGGDETLGKGRAIENRVFIASSGYDYPTYVMDPAGERLSV
                     ASQNGAAAVATIDLNRRYVEKWLGDMRGRFMNELRLDLTVERNRVYVTPSAN"
     gene            complement(5842..6534)
                     /locus_tag="VLE22_25920"
     CDS             complement(5842..6534)
                     /locus_tag="VLE22_25920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17914.1"
                     /translation="MNEPDDNWQLNYFNYFTEVEDHFQRARGTGLFLLSPLDWALIES
                     WKNSGVPLEAALRGIDAAFEKWRSRKSKVQMVNSLAYCSQAVLTEAQAMAGTAPRASK
                     PTSAPFELEEVRNHLSRCSTGLRAQQSSAYHEIASSLDRLAGEADVHYQDLEDFERHL
                     TALEEKMIAIARSTQSEEDLLAARRELDAQLRPYRGKMTADQLAMLEKQYLERRLLES
                     AGLPRLSLFYLR"
     gene            complement(6531..7301)
                     /locus_tag="VLE22_25925"
     CDS             complement(6531..7301)
                     /locus_tag="VLE22_25925"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HSB17915.1"
                     /translation="MLLALDVGNSNITIGAFAGGALAGRWRLRTVIDQTADEWGIFMR
                     NLFSLSSFDLSEVDGIIVASVVPPLDSTLALMAERYFQTTALFVTPDTNTGLKISYEN
                     PSEVGADRIVNGVAAFHKYGGPCVVVDLGTAITFDAVSAGGRYLGGVICPGINIAIEA
                     LFSKTAKLPLVDFREPEKLIGTNTVGSIQSGLYFGSVGLIDGILERLVAELGPETRAV
                     ATGGQAHLIVRGSRFLSEVDEDLTLEGLRLIWERNHPK"
     gene            complement(7313..8068)
                     /locus_tag="VLE22_25930"
     CDS             complement(7313..8068)
                     /locus_tag="VLE22_25930"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HSB17916.1"
                     /translation="MPLDLARLRSRLPGRQVLWFDTVGSTMTEAVRLAVGGAPSGTVV
                     VAEEQVAGQGRHGRSWMSERETGLYLSLILRASPPQESVPVLALALALSAQDAIAKTT
                     GVVCDLRWPNDVIAAGRKCAGILVQLHDGVFVAGIGINVNQTCFPQELSEIATSIRLV
                     SGRPHSREDLFCSLVLATDEFCRVLLEDGKDELLRLYAEASSYVRGRRVTVDQGEALL
                     RGTTEGLNSSGFLILRQDDGTRSVILAGGVRPA"
     gene            complement(8071..8910)
                     /gene="nadC"
                     /locus_tag="VLE22_25935"
     CDS             complement(8071..8910)
                     /gene="nadC"
                     /locus_tag="VLE22_25935"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HSB17917.1"
                     /translation="MSFDITHPDIHETVWRALEEDIGPGDITSEACVPADRKAEGCFV
                     AREPLVVAGTELLPLIYQMRGGIEEVTVAATSGQRAEPGQQIACARGNARTLLECERV
                     ALNFLQRLSGVATTARRYVDEVAGTKCRILDTRKTTPGLRRLEKMSAAAGGVNNHRMG
                     LFDAVLIKNNHTAAAGGVRAALERAYRTGLSVEIEIHSHAELEEALACGAKRILLDNF
                     TPEEVAEHIQFIAGRAEVELSGGINLENVRAYAQTGADFISCGAITHSAVAVDINFRL
                     KLN"
     gene            complement(8910..11546)
                     /locus_tag="VLE22_25940"
     CDS             complement(8910..11546)
                     /locus_tag="VLE22_25940"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HSB17918.1"
                     /translation="MQIEKVYEPQRFEPHWARWWIDSGVFRPSGASERVFALVIPPPN
                     VTGSLHMGHMLEHTEIDVTIRWHRMRGENTLWLPGTDHAGIATQMVVERKLAEEGTDR
                     RTLGREEFERRVWAWKREYGDTIKRQMIRLGVSCDWGRERFTLDPGLSRAVREVFVRL
                     YEKGLIYRGEYMVNWCPRCHTALSDLEVVKEETAGNLWHIRYPVNGSDRALVVATTRP
                     ETMLGDTAVAINPKDERYGDAHGRTVQLPLMDREIPVILDTLADPEFGTGVVKVTPAH
                     DPNDFEAGKRHNLPKIRVIDETGKMTAEAGRYGGLDRFEARLRVVADLDDLGLIEKIE
                     PYHLAIGKCQRCKTVVEPLISTQWFVKMKPLAEPAIRAVEDGRVQFVPENWVKTYNEW
                     MYNIRDWCISRQLWWGHRIPAWYCGDCGAITVSRETPTACVHCGSGWLTQDPDVLDTW
                     FSSALWPFSTLGWPDDTVDLRTYYPTSLLITGFDILFFWVARMIMMGLEFMGDVPFRQ
                     VYIHGLVRDAERQKMSKTRGNVIDPLEVTEKYGTDAARLSLLLGAAPGTDIVLTEERM
                     VSSRAFANKIWNAARFLYLNMERSGVEPEVRGPGAAETLEDRWIFSRLNRCAEAANRA
                     IEQYRYHEVAQVLWQFVWHEFCDWYVEIKKLRFQDNSGLTNDWRNVLSVFEMSLRLLH
                     PVMPFLTEELWQRLTPDKTGRPVSIALAEYPQPDPALANETAEKEMEILQEIITAARN
                     LRADMKADPKQQLDGTLYSRTGALDLAVRHIDVIQKLANVRLELSPGTAPRGGGILRS
                     TPEFDLVLQAPAGQIEAQRQRLEKEIQQLEKVIAGSKRQLGDDTFLSRAPVPVVDGIR
                     RKLADYEAQLEKSRSALEGLPA"
     gene            complement(11644..11940)
                     /locus_tag="VLE22_25945"
     CDS             complement(11644..11940)
                     /locus_tag="VLE22_25945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17919.1"
                     /translation="MLGASSLTILLVVWAAITVVFISLLIWKSFAGMREEDQLFIDPV
                     EAKQEREQREIISKVERIAAWAKGFGFTSAALLLVIAGMWIYQGFTAFNKPPMP"
     gene            complement(12086..14827)
                     /gene="ppdK"
                     /locus_tag="VLE22_25950"
     CDS             complement(12086..14827)
                     /gene="ppdK"
                     /locus_tag="VLE22_25950"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HSB17920.1"
                     /translation="MPKYVYAFGGGTADGHGAMKDTLGGKGAGLAEMSRAGVPVPPGF
                     TIATDVCNIYFNNDKKVPAEIEREAAQALAALEQKMGQKLGDPQNPLLLSVRSGAKFS
                     MPGMMDTILNLGLNDVTVESLARKSGNARWAYDCYRRFIQMFGNVVLEIPKDEFEHIL
                     EARKHKTKVKFDTDLTAEDWKEIVADYKKLVQKKTGEPFPQDALKQLEMARNAVFRSW
                     WNPRASHYRKMNKIADNLGTAVNVQTMVFGNLGDTSATGVGFTRNPATGEKVFYGEFL
                     VNAQGEDVVAGIRTPNPISELRKLMPEVYDQLRLITANLEKHYRDVQDFEFTIQEGKL
                     FMLQTRNGKRTGPAAVRIAVEMVGEGLIGKEEAVMRVDPQQLDQLLHPVLENASKKNL
                     KKLATGLPASPGAAVGKVVFTADDAVAWAKESPVVLVRKETVPDDIHGMEVAKGILTS
                     RGGMTSHAAVVARGMGTPCVAGAESIQVDERKKQLTVQSAGVVLEEGDWISIDGTTGE
                     VFAGQANTVEPDPSSGALAKFMGWADEFRGEFGVRANADIPRDAKVARKFGAEGIGLC
                     RTEHMFFGPGRIEHMQAMILAEDEKSRRKGLAKLLPLQRKDFAGLFQAMNGFPVVIRT
                     LDPPLHEFVPKREDLMVDIAKLPHATVKDKKEMAEKYGMKVGELKKNLPALLHRVEQL
                     HEFNPMLGHRGCRLGITYPEITEMQARAIFEAAAQVKKKGVDVIPEVMIPLIGSVEEL
                     ADQKAIVERVAQEVLQKAGVKNLKYMIGTMIEIPRAALTADRVAGEAEFFSFGTNDLT
                     QTTMGLSRDDYVKFSKDYENKKIFKADPFAVLDQEGVGKLVDMAVKLGRGVRPDLEVG
                     ICGEHGGEPSSVEFCYRVGMDYVSCSPYRVPIARLAAAQAAITSKTAGSKAETMRTA"
     gene            complement(14840..>16146)
                     /gene="glyS"
                     /locus_tag="VLE22_25955"
     CDS             complement(14840..>16146)
                     /gene="glyS"
                     /locus_tag="VLE22_25955"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HSB17921.1"
                     /translation="EALLDTLVYLTEQPAPILGSFDARYLELPEEVLVTVMRHHQRYF
                     SVVGADGKLAPHFVAVMNTSADPDGLVRKGNERVLRARFNDARFFWDVDQHKKLADRV
                     DDLSHVTWQAQLGSYLEKTQRVVVLVSEFGGSEEAQRAAQLAKCDLTTEMVKEFTELQ
                     GVMGGLYARAQGEPEAVWRAVYEHYRPLSMEDSIPSTLEGRVVSLADKLDTLRSCFGI
                     GLMPTGSKDPFALRRAAQGVVKILVEGRMPLALSRLLGTDGQLREFLLDRIRYYFRDV
                     RGFRYDEVNAVLAAGYDDLVDVESRLAALQAVRPTENFEPLAASFKRIRNILRQAEFT
                     GGGEVDEGLLEAGPERELYGAFREALSAIKIVAGLAGSNEPTYENKLKTIASIRPQVD
                     LFFDKVLVNAPDEAIRRNRFTLLHSLLTEFSSIADFSEIVTS"
BASE COUNT         3029 a         5120 c         4843 g         3154 t
ORIGIN      
        1 aagcgcttcg accacagagg cgcgccgtcg gatccccgat acttcgccag gaaaccatcc
       61 ggaggaccag cgttcgttaa attggacccg cccaagttga ggatgctgga ggagaagtat
      121 ccggtgatgg acgggttgcc gctggaatcc actgctaccc cactggtgta atccatacct
      181 gacacgccgc cgtaggcctt ggaccattgg tggctgccgt ccgtccccgc caacttggcc
      241 aagaaaatgc ctccgtaacc gggtcgctga gggctggtaa tcaacccgcc gccaaaattg
      301 atcgcgcatc cttcactggt gctgcagccg aatttcccgg taaggaagac attcccaccg
      361 gcatcaatcg ccagcccgct agcagcgtca ttcccccccg cgttggtgct gccgcctgtc
      421 gctacgctct tgaagggctt ggaccagagg caggacccat ccggcgccag cttggcggca
      481 aaaatggatc cactcaccgt gccgcagcca aaattcgcgt ttgcgaatac tcccgccagg
      541 aaaacgttgc cgtctgcatc ggtcgctacc gccttaccgg acgcatagcc gctgttgaaa
      601 ctcttcgacc aaagtggggt cccgtccggg gcgaacttgg ccaggaacag acaatcgccg
      661 ctggttccgc agaccaaatt ctgaccgccg atattggcgc tacctgtgaa ggaaccggtc
      721 aggtaaacat ttccactcgc gtcggtggca acggcagtgc cctggtcatt ggtgggactg
      781 ccaaaggctt ttgaccacag gtacgcagga tcagcagcca gcgccggttg gaaaccaaga
      841 acaaacgcga aaaacgcgaa caaaacggag ggaatggtac gtatcactgc tcaaaatctc
      901 ctattctcca agtcggaatc gccggctcag gcggttctca tgctcgggcc acgcagaccg
      961 ctagtcaccg ggaattccgt tggagcatga acaggtcgag cgagcatttg atctgactcc
     1021 tccctctcga atcggtctgc cgctcttgca gtctcaatcg taggagtcat tcccgacctg
     1081 gtcaatctcg tgtttagttg ttagtaccta gctaacttaa ggaaacagaa ctagttagca
     1141 atgctcagtt aaccttgcgg gcaccccgcc ggcgaggttg gtcacgcgag tgctgttcgc
     1201 gcgatttacc cgcatctctc ctcggacatt ttggtactac gcgggcacgt gaccggactg
     1261 gtggtggact ggagatgaga agatgcgagt cggcctgccc acacctttcg gagtttcaga
     1321 tgcacttgcc cgctcattgt gactggtaga gcgttagtac tgcacgtcat gagccaatct
     1381 gcctatggtg ataactcatt gaaagcaaaa gcgttgactc ccgtaaacgc ttcgtccaga
     1441 gccgaaacat tggggattat gacgctctgt cggtaataat ttcactgctt cgccagagat
     1501 tcgccctacg gccccgtgta gccccgcacc cgcagcaaat ccgcgatcgc ggtaccggag
     1561 gctcgggcgg gcttgatctc caccggcacc ggaatcagtg cttcggcctt ctcgccatag
     1621 ggtccttgcg atagcacgtt tcctcccggc ccaaccgcga gcgagcaccc gatgcacttg
     1681 cggcccttcc atggacctcc ggtgagccag cctacattgc taacgccgat caccgtcagg
     1741 tcgtacagtt tggcgagcgt cgtgtacgat ttcatccata gcgcgccgta gggtttcttc
     1801 tcgttgtcgt gatccgcatc cacggcccag gcgctcggcg aaagaagcag ccggcacccc
     1861 atgcgcgcca gagagtgcgc caaggcgagg gaatcgggga aattgtcggc gcaaatggtg
     1921 atcccgagaa cgccgagcgg ggtcggcacg acttcgagcc gtccgccggg cgtgtacagg
     1981 ctgtgggcaa tggtcagctc attgattttg cggtgtttga ggaggatctc accagaaggg
     2041 gagacgagta ttgccgcgtt gtggatgcgg tcgccgtctc gctccaccag cccggcggct
     2101 acgtggatac cggcctcgcg cgctgcggct gcgatggctt cactatgagg ccccggaatg
     2161 ggctgagcct tccggcgggc actggggtgg gtccagcaga ggtcgagaca ctccggaagg
     2221 acggcgatgc ggcagccctg ctccgccgcg cacaggatca tggcccgtgc gcgttggaga
     2281 tttcgcgcgg gcttgcctcc ctcgacgagc atctgcccca ttccgagaat gaaactgttt
     2341 gtcattcggg gaacctcagg agcgtatggt cagcaccgcg tagtacgcca taaggcagac
     2401 gatcacgcca gaggcaaacc acagcccgag cgtgacccag cggcgcggga tcacttcttt
     2461 cggcagatgg cgatagcgaa gatagagagc gccgatggcg ataaccggca gcatcagcgc
     2521 ctgggcaacg ccgccggcga ttaccatttt caccggcgac tggaagatga agaacagacc
     2581 aacgggcagg acggtcagca gccaaacgaa ccggctgcgc cagcggagcc tcatcgcgta
     2641 atcgtgggca gggaacagcc ccatcaaccg gcacatgtcg gcgtaaacgc gggagtgggc
     2701 ggcggtggcg gcgaagatgg tgccgtagag cgtcgcgatc gcgccgacat agaagagcca
     2761 cagggaccag gtcccgaggg tctgcgtata gatgtttgag agtacgggga tcatatcctt
     2821 cgccgccggc acgagcccca tgccgttcag aatccccgcg ccaaggaggt agaaggcgac
     2881 ggtggcaacc gcatagatga gcatcgaagc gaagatgtct acgtgcatca cgcggatcca
     2941 gccgagggcg cgccaacgcc acatcggact gccatcgttg cgccccgcac acctcgcgta
     3001 acccttctcc acgcaccagt acgggtacat gaacatctcg gtggcgccaa cgccagtgat
     3061 gccaaacacg gcaacggcgg tcgtaagacc ttcgccgggc atctggaacg tcaggccgct
     3121 gagcagacgg tcccaggaga agaattgcgg catccggatc agcagcagcg cgcagaggaa
     3181 ggtcagcatg gtgaacagtc cgaccttgat catcgccagc ttctcgatgc gctcgtagcc
     3241 gccgccgagc agaagggcca gcgtgatcac gaggaacaga aaaacccagg cgttcacggg
     3301 caccgccgga atgatctggt tcatcacctg ggagacgccg ccgaacatcg cgccgacctg
     3361 gaacagcgtc atcagcacca tgaacgccca cgcccagacg acccatcgaa ccttccagcg
     3421 gggcccgggc acgcgatcaa agccctggag tccggtttct ccggtggcga tggtgtagcg
     3481 gccgagttcc gcctgcaccg ccggcttaat gaaacagctc aggaggatga cccagagggc
     3541 tgcatagccg gcttgggctc cgagcgtggt ggtcgcgatg agctcgcccg atccgacgat
     3601 ggacgcggcc aagatcattc caggcccgat gcgccgcaga atggcgaata gcgtctgtgg
     3661 cgggtcggcg acgtcttccg ggcgtagctc gtacagatcc ttggggacca tctcggtggc
     3721 cggcttggtc attccgagcc actgtacttg attgggcggg agaagttcaa atcgttagga
     3781 cggaggtgcg gtcgcgatgt ccgtctttga gaagtcgtcg cccgtgaaaa gaagcggcag
     3841 tccggagagc cgcgcggttg cgtaggacag acagtcgccg aagttcaggg cggcgggatg
     3901 gcgacccttt ccgtatcgaa ggaatgcgtc gaccgcaacg tagtaatggt ccagcgtaaa
     3961 ggggacaacc tccgcatcgg cttcgcgaag aaagtcggtg agcagccccc gtgcgtcacg
     4021 gcgcaagcgt gaggacaaca caatcgcact ttcgactagg gtcggtacac ccactgctac
     4081 caccggcgcc tcgccgatcc ggttgaggat cggctcgtgc ccaggctcct gaagcaggac
     4141 cgccagtatg gctgatgcgt ccaaaaccat gcgctagacg ccctcggagc cgtagccaag
     4201 aatttgctcc cgctcggcgc gactcagtcg ggtcccgcgc agtctggcag gaatctgtgg
     4261 ccagatttgt tcgcgcagca aagcttccat ccgcgctttc cggcttatgt tccctctgcg
     4321 agcctgaaga cgaagcttcc ggtcgagcaa cgcgcgccgg attgcctccg tctttgtttc
     4381 tttcgccagc cgggccacct cggcggcgag actctctacc tccgaattct tgatgttcag
     4441 agccatggtg taagtatacc ctaattatac catgcttcaa ttcgcgctcg gcgttacata
     4501 aactcggttc cgctccaccg tcagatccag gcgaagctcg ttcataaagc ggccgcgcat
     4561 gtcaccgagc cacttctcga cataacgtcg gttaaggtca atggtggcga cagcggcggc
     4621 cccgttttgc gacgccacgg agagccgctc accggccgga tccataacgt acgtagggta
     4681 gtcgtagccc gaggaggcga tgaagacgcg gttctcgatg gcgcgtccct ttccgagtgt
     4741 ctcgtcgcca ccccagatgg gcagaagcag gagctcggcg ccccgcagcg ccagcgctcg
     4801 cgatgggtcg gcgaactgga ggtcccagca gatcatcatg ccgacgcggc cgaagtcggt
     4861 ctcgaaaacc gggtagtcga gaccaggagt aatgccgccc tccaattcct cccgcgggat
     4921 atagacctta cgatagcggc cgaccacttt tccggcccta tcgatcagca cggcggtgtt
     4981 gtacacagct gagccctccc gctcgtagat cccggcggcg acgtaagcgt tgtgccggcg
     5041 agcagcctcg ccgagcctct ccgtcgtcgg tccgggcacc ggttccgcga cgtctacata
     5101 cgttttgccg gtgccgacga tcgtgattcc ctccggcagc agaatgacgt ccaccttggc
     5161 tggagcggtc ttgtccacga cttcgacgaa cctccgcacg ttctccgccg ctgacttgga
     5221 gccgcttggg cggaagttta tcgaggcgac agtgacgttg cgcggcggcg gagccggcac
     5281 ttcctcgagg gagatgtcgt cccaccacac ggtggcttgt ggagaattga gcaggaacag
     5341 ctgcaccctt actgccgttg ccttctcagg cgccggcgcc tgagtggtga tgcgattcca
     5401 accttcgact ggagtggttg cgtaaccgta ctcgggctgt ccggcccgcc ggccgtccgc
     5461 cgcggcccag tcgagccgcg cccagagatg gagcggctga taaatctgcc cctcggtacg
     5521 gtagtgggcc acgagacgat accacttgcc gggttgcact ccggaggccg tgtactgcca
     5581 gccgccgaac tcagccgggt tgctgttgcc ggagatggcg agagagccct gttgcccgcg
     5641 gctgtgcacg gcgtcgacaa atgtccgcgg tgcgatctcg gcgcgcggcg accatgccgt
     5701 ccacccttcg ggggtgccgt ccggccctgc gttgaacccg gactgacgga acggcaacga
     5761 tccagcgaga acgacgcccg ccgtgcaaag cagtaataca agtgacctca tgaagccaag
     5821 tatagcgaga gcccctggcg tctatcggag gtagaacagg ctgaggcggg gaaggcctgc
     5881 cgattcgagt aggcggcgtt cgaggtactg cttctccagc atggcgagtt ggtcggcggt
     5941 catcttgccc cggtagggac ggagctgggc gtccaactcg cgccgtgccg cgagaaggtc
     6001 ctcctcgctc tgcgtgcttc gggcgatggc gatcatcttc tcttcgagag cggtcaggtg
     6061 gcgttcgaaa tcctctaggt cctggtagtg aacgtccgct tcgccggcga gacggtccag
     6121 actggaggcg atttcatggt atgcggagct ttgctgtgcc cgaagaccgg tcgagcatcg
     6181 cgaaagatgg ttccggactt cctccagttc gaagggcgcg gaggtgggtt tcgacgcacg
     6241 cggcgccgtc ccagccatcg cttgcgcttc ggtcagcacc gcctgggaac agtaggcaag
     6301 cgaattcacc atttgaacct tggacttccg gctgcgccac ttctcgaatg ccgcgtcgat
     6361 gccgcgaagc gccgcctcca ggggtacacc gctgttcttc cagctttcga tcaaggccca
     6421 atcgagcggt gaaagaagaa agaggccggt gcctctcgcg cgctggaaat ggtcttcgac
     6481 ttcagtgaag tagttgaagt agttgagctg ccagttgtcg tcgggctcat tcacttcggg
     6541 tggttccgtt cccaaatgag gcgtagccct tccagtgtaa ggtcctcgtc cacttccgaa
     6601 agaaaccgtg agccgcgaac gatcagatgg gcctgtccgc cagtcgccac cgcacgggtt
     6661 tccggaccca gttcggcgac gagccgttcc agaatcccgt cgatcaaccc gaccgagccg
     6721 aagtacagtc cggattggat acttccgacg gtgttggttc caatgagctt ctccggctcc
     6781 cggaaatcca ccagtggcaa cttggcggtc ttggagaaca gagcttcgat ggcgatgttg
     6841 atgcccgggc agatcacgcc gccgagatac cggccgccgg cggagacagc gtcaaatgtg
     6901 atcgccgttc cgagatcgac gacgacgcac gggccaccgt acttgtggaa cgccgcgacg
     6961 ccattcacga tgcgatccgc ccccacctcg cttggattct cgtaggagat cttcagcccg
     7021 gtgttcgtat ccggcgtcac aaacagcgcg gtggtttgga aataacgttc cgccatcagc
     7081 gccaaggtcg aatcgagggg aggcacgacg ctcgcgacaa tgatgccatc cacttcggag
     7141 agatcgaacg aggagagcga gaacagattc cgcatgaaga tgccccactc gtcggccgtc
     7201 tgatcgatga ccgttctaag acgccatcgt ccggccagag cgccgcccgc gaaggctccg
     7261 atggtaatat tgctgttccc tacgtccaac gcaagcagca tggcgttcct tttcaggccg
     7321 ggcgcactcc gcccgcaaga atcacactcc gcgtgccgtc gtcctgtcgc agaatcagga
     7381 aaccggagga attgagccct tcagtggttc cgcgcagcaa cgcttcgccc tgatccaccg
     7441 tgacgcgacg gccccgcacg tagctcgatg cctctgcata aagacggagg agttcgtctt
     7501 tgccgtcttc caagagaacc cggcaaaatt cgtcggttgc caggaccaga ctgcaaaaca
     7561 ggtcctcacg cgaatgaggc cgtcccgaaa ccaatcggat ggacgtggcg atctccgaca
     7621 actcctgtgg gaaacacgtc tggttcacgt tgattccgat tcccgccacg aagacgccgt
     7681 cgtgcaattg caccaggatg ccggcgcatt tccgtcccgc cgcgatcacg tcgttgggcc
     7741 agcgcaggtc gcacaccacg cccgtggtct tcgcgatcgc atcctgtgcc gagagcgcca
     7801 gtgcgagcgc cagcactggg acggactcct ggggcgggga agcccggagg atgagggaca
     7861 gatacaggcc ggtttcccgt tccgacatcc atgagcggcc gtggcgtccc tgcccggcga
     7921 cttgctcctc cgcgacgacg accgtcccgg acggcgcacc tccaaccgcc agccggacag
     7981 cttccgtcat ggtagacccg accgtgtcaa accacaagac ttgccgtcct ggcagcctgg
     8041 agcgaaggcg ggcaagatcg agtggcattg tcagttgagc ttcagccgga agttgatatc
     8101 cacggcgacg gctgaatgcg tgatggcgcc gcaggagatg aagtcggccc cggtctgcgc
     8161 gtatgctctg acgttttcga gattgatgcc acccgacagc tcgacttcgg cgcggccggc
     8221 aatgaattgg atgtgctcgg ccacttcctc cggtgtgaaa ttgtccagca gaattcgctt
     8281 tgctccacaa gccaatgctt cttcgagttc ggcgtgactg tggatctcga tctcaaccga
     8341 gaggccggtg cggtacgctc gctccaaggc cgcccggacg cccccggcgg cggcggtgtg
     8401 gttgttcttg atgagcaccg cgtcaaacag gcccattcgg tggttgttga cgccgcccgc
     8461 ggcagctgac atcttttcaa ggcgtcgcag gccaggggta gtctttcggg tgtccagtat
     8521 ccggcacttg gtgcccgcga cttcgtccac gtaccgccgc gcagtcgttg ctacaccgct
     8581 cagccgctga agaaagttca gcgccacgcg ctcgcattcg agaagcgtcc gggcattccc
     8641 tcgtgcgcac gctatctgct gtcctggttc ggccctctgt ccgcttgtcg cggccactgt
     8701 gacctcttcg ataccgcctc gcatctgata gatgagcggt aacagttcgg ttcccgccac
     8761 gaccagcggc tcccgagcca cgaagcaccc ctcggcttta cggtccgccg gaacgcaggc
     8821 ctctgacgtg atgtcgccgg ggccgatatc ttcctcgagc gctcgccata cggtttcgtg
     8881 aatatcggga tgcgtgatgt caaaactcat caggccggaa gtccttccag tgcagagcgg
     8941 ctcttctcta gttgcgcttc atagtccgcc agcttcctcc gaatgccatc aacgaccggt
     9001 actggcgccc gactgaggaa ggtgtcatcg ccaagctgcc gcttcgatcc ggcgataacc
     9061 ttctcaagtt gctggatctc cttctcgagc ctttgccgtt gggcttcgat ttggcctgcc
     9121 ggcgcttgca ggacgagatc gaactccggt gtcgagcgaa gaatcccgcc gcctctcggg
     9181 gccgtgcccg gacttagttc cagcctcacg ttagccagct tctgaatgac gtcgatgtgt
     9241 cgcacggcca ggtccagagc tcccgttcgc gagtacaacg tgccgtcgag ttgctgcttc
     9301 ggatcggctt tcatgtccgc gcgaaggtta cgggccgcgg tgatgatctc ctggagaatc
     9361 tccatctctt tctcggcggt ttcgtttgcc agtgcaggat cgggctgtgg atactctgca
     9421 agggcgatcg agaccggccg gccggtcttg tcgggagtta accgctgcca aagttcctcg
     9481 gtcaggaagg gcatcacggg gtgcagcaga cgcagcgaca tctcgaaaac gctcagcacg
     9541 ttacgccaat cattcgtgag gccggagttg tcttgaaatc gcagcttctt gatctcgacg
     9601 taccagtcgc agaactcgtg ccagacgaac tgccacagga cctgcgccac ttcgtgatat
     9661 cgatactgtt cgattgcgcg gttggccgcc tccgcgcacc ggttgaggcg gctgaaaatc
     9721 cagcggtctt cgagcgtctc ggcggcgcct ggtccgcgta cctccggttc cacgccggaa
     9781 cgctccatgt tcaagtacag gaagcgggcg gcgttccaga tcttgttggc gaacgccctt
     9841 gaactgacca tccgctcctc ggtcaggacg atgtcggtgc ccggagccgc ccccagcagt
     9901 aaggagagcc tggccgcgtc ggttccgtac ttctcagtga cttccagcgg gtcgattacg
     9961 ttaccgcgcg tcttggacat cttctgccgc tcggcgtccc gcacaaggcc gtggatgtag
    10021 acctggcgga atggaacgtc gcccatgaac tccaggccca tcatgatcat ccgtgccacc
    10081 cagaagaaca ggatgtcgaa accggtgatc agcaaggaag tcggataata tgtccgcagg
    10141 tcgacggtgt cgtccggcca cccgagcgtc gagaacggcc agagcgcgga actgaaccaa
    10201 gtgtcgagca catcggggtc ttgcgtgagc cagccggaac cgcagtgcac gcatgctgtc
    10261 ggagtttcgc gggacaccgt aatggcgcca caatcgccgc aataccaggc cgggatcctg
    10321 tgtccccacc agagttggcg cgagatgcac cagtcacgga tgttgtacat ccactcgttg
    10381 tacgtcttga cccaattctc cggtacgaac tgcacgcgcc cgtcttccac cgcacggatg
    10441 gcgggctcgg ccagcggttt cattttcacg aaccattgag tggagatgag cggttcgacc
    10501 accgtcttac agcgctggca ctttccgatg gcgagatggt acggctcaat cttctcgatg
    10561 agaccgaggt cgtcgaggtc ggccacgact cgcaggcggg cctcgaagcg gtccaggccg
    10621 ccgtatcgac ccgcctcggc ggtcattttc ccggtctcgt cgatgacgcg aatcttaggc
    10681 aggttatggc gcttgccggc ctcgaaatcg ttcgggtcgt gagcaggagt caccttgacg
    10741 acgccggtgc cgaactcggg gtctgccagt gtgtccagaa tcacgggaat ttcccggtcc
    10801 atgaggggca actgcaccgt tcggccgtgc gcatccccgt accgctcgtc tttcgggttg
    10861 atagcgaccg cggtatcgcc aagcatcgtt tcgggccggg tagtggcaac aacgagggcc
    10921 cggtcagatc catttaccgg atagcggatg tgccacaggt tgcccgcggt ttcctccttc
    10981 actacctcga ggtcagagag ggcggtatga cagcgcgggc accagttcac catgtactcg
    11041 ccgcggtaga tgagcccttt ttcgtacagg cgaacgaaca cctcacgaac tgcgcgggaa
    11101 aggccgggat ccagcgtgaa gcgctcacgg ccccagtcgc aactgacgcc gagccggatc
    11161 atctgtcgct tgatggtatc gccgtactcc cgtttccagg cccacacgcg ccgctcgaat
    11221 tcctcgcggc cgagcgtgcg ccggtccgtg ccctcctcgg ccagtttccg ctccaccacc
    11281 atctgcgtgg cgatacccgc gtgatcggtt ccgggtagcc agagcgtgtt ctcgccccgc
    11341 atccggtgcc agcggatggt gacgtcgatc tcggtgtgtt ccagcatgtg ccccatgtgg
    11401 agcgagccgg tcacgttcgg cggggggatg accagagcaa aaacgcgctc cgaggctccg
    11461 gagggacgga acacgccgga atcgatccac caccgtgccc agtgcggctc aaaccgctgg
    11521 ggttcataga ctttctcgat ttgcatcggg aaatttcaat atatcacgcg agtgccggcg
    11581 gacccttcgt acgtgcagac acaccttcga tatacgggac aaccgggagt agccgctggt
    11641 cgtctatggc atgggtggtt tgttgaaggc tgtgaatccc tggtagatcc acatgccggc
    11701 gataacaagc aggagcgctg cggacgtgaa gccgaaccct ttggcccaag ccgcgatccg
    11761 ctcgaccttg gagatgatct cccgttgctc ccgttcctgt ttcgcttcga cggggtcgat
    11821 gaaaagctgg tcctcctccc gcattccggc gaacgatttc cagatcagca gggaaatgaa
    11881 aacgaccgtg atggccgccc aaacaaccag cagaatagtg agagatgaag caccaagcat
    11941 ggatttcctc ctggccaggt gttttcagcc ctcatactgg accgaaagac cgttcatgtc
    12001 aagggggtag cgggaaccgg agccgctgag agtcgactgg aggggaagga tgggggcagg
    12061 gcgcgcagcc tgcccccggg agtgcttacg ctgtgcgcat cgtctcggcc ttggagccgg
    12121 ccgtctttga tgtaatggcc gcctgcgctg ccgccaggcg agcgatcggc acacggtacg
    12181 gggagcacga aacgtagtcc atgcccaccc ggtagcagaa ttcgaccgag ctcggctcgc
    12241 cgccgtgctc gccgcagata ccgacttcga ggtccggcct cacgccccgg cccagtttca
    12301 cggccatgtc cacgagcttg ccgacgcctt cctggtccag caccgcgaac gggtccgcct
    12361 tgaagatctt cttgttctcg tagtccttcg agaacttgac atagtcgtcg cgcgacaggc
    12421 ccattgtcgt ctgcgtgagg tcgttggtgc cgaagctgaa gaactcggct tcgcccgcca
    12481 cccggtccgc cgtcagcgcc gcgcggggga tttctatcat cgtcccgatc atgtacttca
    12541 gattcttcac gccggctttc tgaagaactt cctgggctac gcgctccacg atcgccttct
    12601 ggtctgccag ctcttcgacg ctgccgatga gcggaatcat gacctcgggg atcacgtcca
    12661 cgcccttctt cttcacctgt gcggccgctt cgaagatcgc ccgcgcctgc atctcagtga
    12721 tctccggata agtgatcccc agcctgcagc cccggtgtcc gagcatcggg ttgaactcgt
    12781 gcaactgctc gacgcggtgc agcagcgccg gcagattctt cttcagctcg ccgaccttca
    12841 tgccgtactt ctccgccatc tctttcttgt ctttgacggt ggcgtgcggc agcttggcga
    12901 tgtccaccat caggtcttcc cgcttcggca cgaactcgtg cagcggcggg tccagcgtgc
    12961 ggatcaccac cgggaagccg ttcatcgcct ggaacaatcc ggcgaagtcc ttccgctgga
    13021 gcggcagcag cttcgcgagc cccttccggc gcgatttctc gtcctcggcc agaatcatcg
    13081 cctgcatgtg ctcgatgcgg cccggtccga agaacatgtg ctccgtgcgg cagagaccga
    13141 tgccctcagc cccgaatttc cgggcgacct tcgcatcgcg tgggatgtcc gcgttggcgc
    13201 gcactccgaa ctcgccgcgg aactcgtccg cccagcccat gaacttcgcc aacgcacctg
    13261 acgacggatc gggctcaacc gtattcgcct ggccggcgaa cacctcgccc gtcgtgccgt
    13321 caatcgaaat ccagtcgcct tcctccagta cgacgcccgc gctctgcacc gtgagctgct
    13381 tcttgcgctc gtccacctga atcgactcgg cgcccgccac gcacggcgtg cccatgccgc
    13441 gcgcgaccac cgccgcgtgg ctcgtcatac cgccgcgcga tgtcaggatg cctttcgcca
    13501 cttccatgcc gtggatgtcg tccggcaccg tttccttgcg gaccagcacc accggagact
    13561 ccttcgccca tgccaccgcg tcgtccgcgg taaagacaac cttgcccacg gcggcgcctg
    13621 gcgaagccgg cagcccggtc gcgagcttct tcaagttctt cttggaggcg ttctcgagca
    13681 cggggtgcag aagctggtca agctgctgcg ggtccacgcg cattaccgcc tcttctttgc
    13741 cgatcagacc ttcgccaacc atctctacgg cgatgcggac ggccgccggg ccggtgcgct
    13801 tgccgttgcg ggtctggagc atgaacagct tgccttcctg gatggtgaac tcgaagtcct
    13861 gcacgtcgcg gtaatgcttt tccaggttgg ccgtgatcag gcggagctga tcgtacacct
    13921 cgggcatcag cttccgaagc tcggagatcg gattcggcgt gcggatgccc gccacaacgt
    13981 cttcgccctg cgcgttcacg agaaactcgc cgtaaaacac cttctcgccc gtcgccgggt
    14041 tgcgtgtgaa gcccacgccc gtcgccgacg tgtcgcccag gttcccgaat accatcgtct
    14101 gcacgttgac cgcagtgccc agattgtccg cgatcttgtt catcttccga tagtgtgaag
    14161 cccgcgggtt ccaccaggag cggaatacgg cgttgcgcgc catctcgagc tgcttgagag
    14221 cgtcctgcgg gaacggctcg ccggtcttct tctggaccag cttcttgtaa tcggcaacga
    14281 tctctttcca atcttcggcc gtgaggtcgg tgtcgaactt caccttcgtc ttgtgcttac
    14341 gcgcttcgag gatgtgctcg aactcgtcct tcggaatctc gagcactacg ttgccgaaca
    14401 tctggatgaa gcggcggtag cagtcgtatg cccaacgggc gttgccgctc ttccgcgcca
    14461 gcgactccac cgtgacgtcg ttcaagccca ggttcaggat cgtgtccatc atcccgggca
    14521 tcgagaactt cgcgcccgaa cgaacgctca ggagaagcgg gttctgaggg tctcccagct
    14581 tctggcccat cttctgttcc agggccgcca gggcctgcgc cgcctctctc tcgatctcgg
    14641 cggggacttt cttgtcgtta ttgaagtaga tattacagac gtcggtggca atggtgaaac
    14701 ccggggggac cggcacgccg gcccggctca tctccgcgag gcctgcgccc tttccgccga
    14761 gggtgtcttt cattgcgccg tggccgtcgg ccgtaccacc gccgaaggca taaacgtatt
    14821 taggcatatt ggtgtctcct taggaagtca cgatttcgga aaaatcagca atcgacgaga
    14881 actccgtcag aaggctgtgc agcaatgtaa atctgttccg ccggatcgct tcgtccggcg
    14941 cattcacgag aaccttgtca aagaagaggt ccacctgcgg gcggatgctc gcgattgtct
    15001 ttagcttgtt ctcgtaagtc ggttcgtttg atccagcaag tccggcaacg atcttgatag
    15061 ctgacagtgc ctccctaaaa gcaccgtata actctcgttc cgggccggct tccagcagcc
    15121 cctcatccac ttcgccgccg ccagtgaatt ccgcctgccg gaggatgttg cgaatccgct
    15181 tgaaactcgc cgccagaggc tcgaagtttt ccgtcgggcg gaccgcctga agcgctgcca
    15241 gccggctctc cacatccacg agatcgtcgt atcccgccgc cagcaccgca ttgacttcgt
    15301 catagcgaaa accgcgaacg tcgcggaagt agtagcggat gcggtcaagg agaaactcac
    15361 gaagctgacc gtccgtgcca agaagtcggg aaagcgccag cggcatgcgg ccttctacca
    15421 ggatctttac cacgccttgc gcagcacggc gaagcgcgaa cggatctttc gagccggtgg
    15481 gcatcaggcc gatgccgaag cagctccgga gcgtgtcaag tttgtcggcc agcgaaacca
    15541 cccgcccctc gagcgtggac ggaatcgagt cctccatgct tagcggcctg tagtgttcat
    15601 agacggcgcg ccacacagct tccggttcac cctgtgcgcg cgcatagagg ccgcccatga
    15661 cgccctgaag ttcggtgaac tccttcacca tctccgtggt cagatcgcac ttggccagtt
    15721 gcgccgcgcg ctgcgcttcc tccgagccgc cgaactcgct cactagcacc accacgcgct
    15781 gcgtcttctc gaggtaggag ccgagctgcg cctgccaggt gacgtgcgag aggtcgtcga
    15841 cgcggtctgc cagcttcttg tgctgatcca cgtcccagaa aaaccgagcg tcgttgaacc
    15901 gcgcacgcag cacacgctcg ttgcccttgc gcaccagccc gtccgggtcg gcgcttgtat
    15961 tcatcaccgc gacgaagtgc ggggccaact tgccgtccgc acctaccacc gagaagtacc
    16021 tctggtgatg gcgcatcacc gtcaccagaa cctcttcagg aagctcgagg taccgtgcgt
    16081 cgaaacttcc gaggatcggc gccggttgtt cggtgaggta taccagcgtg tcgagtaacg
    16141 cctcat
//
LOCUS       DATIFQ010000515         9698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2475103, whole genome shotgun sequence.
ACCESSION   DATIFQ010000515 DATIFQ010000000
VERSION     DATIFQ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2475103"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VLE22_25960"
     CDS             <1..526
                     /locus_tag="VLE22_25960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17922.1"
                     /translation="GAIVPLTFVPPFPVYPPETAWMREPKTDIPGLVLSEHREGGRVA
                     YLPADIDRRFAKDHLPDHGNLLANIVRWAGKSDVPLRVEGLGLIDCHLYRQPGRLILH
                     VVNLTSAGTWRAPIDELIPIGPVKVSVRLPSEVRGGGVRLLVSGASPVSGVRDGWCAF
                     ELRSILDHEVAVIG"
     gene            557..2917
                     /locus_tag="VLE22_25965"
     CDS             557..2917
                     /locus_tag="VLE22_25965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSB17923.1"
                     /translation="MGTHQPGGFAQIGGPPVPSRQNTVKDPVCHMDVDPRTAAATYEY
                     KGETYYFCSVHCQKKFAADPGAYLAAKPEKPEPAGRKDAIYVCPMHLEVRQIGPGACP
                     KCGMALEPEIATGEEGPNPELVDMTRRFRVSLWLTIPVFLIAMSEMIPGQPVQRALSQ
                     QMLSYIQLALATPVVLWGAKPFFERGWASIINRNLNMFTLIAIGIGTAYLYSVAATLA
                     PGLFPESFYGHGGGPAVYFEAAAVITTLVLLGQVLELRARERTGSAIKALLGLAPKMA
                     QWLLPDGSEEKIPLEAVHPGYKLRVRPGEKVPVDGVVIDGSSSVDESMITGEPIPVEK
                     AAGSRVTGGTLNGTGSFVMRAQRVGSETLLAQIVRMVVEAQRSRAPIQRLADVVSGYF
                     VPIVVLVAVITFVVWALVGPEPTMAYAIVNAVAVLIVACPCALGLATPMAVMVGTGRG
                     ATAGVLLKNAEALELLEKVDTLVVDKTGTLTEGKPALASVATLPGVKESELLRLAAGL
                     ERGSEHPLAAAIVAGAEARGLNLPAVEGFRSLTGRGVAGVLEGRDVALGNPLLFSELA
                     IESRVFDETAEKLRGQGQTIVYVAVDGRPAGLLGVADPVKKSAREAIQLLHKEGIRVV
                     MATGDNPTTAGVVARELGIDDVRAGVLPDQKSNVVKQLQAEGRVVAMAGDGINDAPAL
                     AQAHVGIAMGTGTDVAMESAGITLVKGDLRGVVRARKLSRGTMRNIRQNLFFAFAYNT
                     LGVPVAAGVLYPFFGLLLSPMIASAAMTFSSVSVISNSLRLRRLKL"
     gene            complement(2936..3964)
                     /locus_tag="VLE22_25970"
     CDS             complement(2936..3964)
                     /locus_tag="VLE22_25970"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSB17924.1"
                     /translation="MTALRIPPGGLLYRAMIGVGGIGSGRFFALDGNETLGREESRGG
                     RFLNQRDYCKLHIISHYVSRLLGRDFATIPVGKIGNDDVGERLLVEMAEAGLDLRHVI
                     REPDAPTLFSFCFVYPDGSGGNMTTNDSACSRVDAAYVLRVEPEFQRYEGCGVALAAP
                     EVPIEARTTLLRLGTAHGFFRVASFTSVELPGAIDSGLISRIDLLALNRDEAVVAAGL
                     ASAGRQPEEIAAAAVERLRALNPQIALLVTAGRDGSWTWDGERLRHVGVPAVPVKGTA
                     GAGDAHLAGVIAGLAAGLPLVEAHHLGALVAAASVTSPHTIHPDLDADLLRTLPTPLR
                     ESVARLLR"
     gene            complement(3961..4872)
                     /locus_tag="VLE22_25975"
     CDS             complement(3961..4872)
                     /locus_tag="VLE22_25975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17925.1"
                     /translation="MADFTPYDLYQWCRIPYQQLETHPARKVQFRLCRDSEEMGRIMA
                     RELVDEIRLHNSRGEATRAIIPCGPACWYQPFTSLVNREGVSLKNFVVFHMDECLDWQ
                     GRELPRAHPYSFRGFMEKHFYDPVDSALAVPESNRFWLNAGNVEEVRRKIWAAPVDIT
                     YGGWGQDGHIAYNQTRRHPFSRITLDDLRNSTVRVQDNNLDTILALAQRTFGSAYQFV
                     PPMSVTLGIKECLSAKKVRLFSDTGAWKQTALRVALFGPLTTEYPITLLQEHPDALIT
                     ATIATASHPVSEHPEWDLGLSAPDRTA"
     gene            complement(4946..6259)
                     /locus_tag="VLE22_25980"
     CDS             complement(4946..6259)
                     /locus_tag="VLE22_25980"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HSB17926.1"
                     /translation="MPEDILFQPLPFRNLTVKNRILRSNMAGRFDNYDGSGNPARINW
                     ELKFARGGVGAIISSFVPVQMRGRIIPNYAAIDRDDRIPFWRELGRRVHEHDCKFILQ
                     LSHAGRQRDIGGIEYAKGLSSTDRPEPFHGFECERMTLAQIRQTVDAFAAGARRAREA
                     GLDGVELHAANGYLFTQFLSSAINDRKDKYGGSLHNRARFLLEVVAAIRAEVGRDFHL
                     QAKLSTTEFNNALVPWESPGNTIKDSLNVCRWLEEAGVDAIHVSSGSAFPHPKNPPGD
                     FPVEEAVKTYDSMLSSGSGALRNYLTFRSGPAAQLYRSVWIKARGETIEGINIPDAIA
                     VKQAVGIPVLCTGGFQTASFIRQALENGVCDAVTIARPLIANNDLAHVFASGKDRPDR
                     PCTYCNKCLVNVLENPLGCYDETRYASREAMIEEILSVFRPAPFA"
     gene            complement(6333..7154)
                     /locus_tag="VLE22_25985"
     CDS             complement(6333..7154)
                     /locus_tag="VLE22_25985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17927.1"
                     /translation="MWGRCVILLSMLTLSLVGQTQPAAEGETQKGWPKAEAPEPGSLV
                     IQSGTRIPLSLINSVSTKHSAEGDRVYLETVFPILADGRVVIPPGSYVAGTITQVKRP
                     GRVKGRGELYLRFDSLTLPNGVTRDFRARVGSLDGRSSEELDRTEGKIKSEGNKSGDA
                     RSVGEATAAGASVGVIAGAGAGHAGMGAGIGAAAGATAGLMGVLLSRGPDAILARGTT
                     IEMVLDRPISFTLEEVNFSGALMPRPTASTGNEGPQPSQKSRGVPLPGRVGRLPY"
     gene            7273..9324
                     /locus_tag="VLE22_25990"
     CDS             7273..9324
                     /locus_tag="VLE22_25990"
                     /inference="COORDINATES: protein motif:HMM:NF045042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17928.1"
                     /translation="MLTVRRFFLLSLLFILISAPPALTADLFRDDFSKFPAGWLTQPI
                     GQINAAIQEYHYLPHRGVPLGLWENALCHMDAWLISEEDGKAYLEQALVNDRPRQANP
                     IFVTGDPEWTDYTVEAKVKPLLRSDMAGLVFRYHTNRHYYLFALTGGNQARLAVRLPI
                     EKTFRIAEWRELGRAEFNYDARVYHTLKVENAGPRIRASIDGKVLIEAEDGEILKGKA
                     GLTSNIPARFQDFVVTVPDAVRSGIDDRIRKREAELARLRSENPRPKLWKKFSTPVFG
                     AGRNARFGDLDGDGKLDMLIAQNVRRFPYDAAHLTCLTAVNFDGKVLWQSGKPDARNG
                     LLTYDNPFQIHDIDGDGRAEVVLVKDFLLQVLDGRTGKLIQRVPTPQAPEGDRNQVYE
                     NVSGDSIAFVNVLGDPRRHEILFKDRYRNFWVYNNKLELLWTGSGQTGHYPYPFDADG
                     DGRDEILIGYAMWDHDGKPLWSHDKTLRDHADGIMMANLSPDPKAEPRAYAGGSDEGF
                     LMFDVRGTLLKHVRLGHGQSPVAGKFRPDVPGLQFATVNFWYNPGIVTMFDWDGNTLA
                     QEEPIHSGSVMLPVNWRGDGQEFILLSGNSREGGMIDGHLRRVVMFPEDGHPDLTAYV
                     ADVTGDPRDEVILWNETEVWIYTQDRPFTGKRIYAPIRNPDYNESNYRVAVSLPRWVD
                     R"
     gene            9398..>9698
                     /locus_tag="VLE22_25995"
     CDS             9398..>9698
                     /locus_tag="VLE22_25995"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HSB17929.1"
                     /translation="MRNLRVASVQFECKPGDKTANLATIRRFAKEAARAGAEILVTPE
                     CSITGYWFLRNLGREEFAALAEPVFDGPSSQALMAMSKEYGMTIGAGLIERGDDGK"
BASE COUNT         1756 a         2884 c         3278 g         1780 t
ORIGIN      
        1 aggggcgatc gtgccgctga cgttcgttcc gccgtttccg gtgtatccgc ccgagacagc
       61 gtggatgcgc gagccgaaga cggacattcc ggggctggtt ctgagcgagc accgcgaagg
      121 cgggcgcgtg gcctatctgc ccgctgacat cgaccggcgg ttcgccaagg atcatctccc
      181 ggatcacggc aacctgctcg ccaatatcgt gcgatgggcg ggtaaaagcg acgttccgtt
      241 gcgcgtcgag ggactgggcc tgatcgactg ccacctgtac cgccagccgg gtcggctcat
      301 tctgcacgtg gtcaacctga ccagcgccgg aacgtggcgc gctccaatcg atgagctgat
      361 tccgatcgga ccggtgaagg tgtccgtccg tctcccgagc gaggttcggg gcgggggagt
      421 tcgtcttctc gtttcggggg cgagtcccgt atccggtgtc cgggatggat ggtgcgcgtt
      481 tgagttgcgc tccattctgg atcacgaggt ggcggtgatc gggtagtcgc tcccccactt
      541 ctggttgaat aggagtatgg gtacacacca acctggggga ttcgcgcaga ttggcggtcc
      601 gccggtcccc tcgcgtcaga acaccgtcaa ggaccccgtc tgccacatgg atgtggaccc
      661 tcgcacggcc gcggcaacgt acgaatacaa gggcgagacg tactactttt gctcggttca
      721 ctgccagaag aagttcgccg cggaccccgg cgcctatctc gcagccaaac ccgagaagcc
      781 ggagccggcc ggccggaagg atgccatcta cgtctgtccg atgcacctgg aggtgcggca
      841 gattggcccg ggggcttgcc caaaatgtgg catggcgctg gagccggaga tagcgaccgg
      901 cgaggagggg ccgaatccgg aactcgtcga catgacacgg cggttcaggg tgagcctgtg
      961 gctgacaatt ccggtttttc tgattgcgat gtcggagatg atcccgggcc agccggtgca
     1021 gcgggccctc tcccagcaga tgctgagcta cattcagctc gcgctggcga cgccagtcgt
     1081 actctgggga gcaaagcctt tttttgaaag aggttgggcg tcaattatca acagaaactt
     1141 gaacatgttc acgctgatcg ccatcggtat cgggacggcg tatctgtaca gcgtggcggc
     1201 cacactcgcc ccaggcctgt ttccggagtc gttctacggg cacggcggcg gtccggcggt
     1261 ctatttcgag gcagcggcgg taatcacgac tctggtgctg ctgggtcagg ttctggaact
     1321 gcgggcgaga gagaggacgg gaagcgcgat caaggcgctt ctcgggttgg ctccaaagat
     1381 ggcgcagtgg ctgctgccgg acggctctga ggagaagatt ccgctcgagg cggttcatcc
     1441 cggatacaag ctgcgggtgc gtccgggcga gaaggtgccg gtggacggcg tagtgatcga
     1501 cggatcgagt tcggtggacg agtcgatgat cacaggcgag ccgatcccgg tggagaaggc
     1561 ggcgggcagc cgggtcaccg gcggcacgct gaacggcacg ggcagttttg tcatgcgggc
     1621 ccagcgcgtc ggcagtgaaa cgctgctggc gcagatcgtg cgcatggtcg tcgaggcgca
     1681 gcggtcccgg gcgccgatcc agcggctggc cgacgtcgtc tcgggctact ttgtgccgat
     1741 cgtggtgctg gtggcagtga tcacgtttgt ggtctgggcg ctcgtggggc cggaaccgac
     1801 gatggcgtac gcgatcgtca acgcggtagc ggtgctgatc gtcgcctgcc cgtgcgcact
     1861 cgggcttgcg accccgatgg cggtgatggt cgggacggga cgcggggcga cggctggtgt
     1921 gctgctgaag aatgccgagg cgctcgagct tctggagaag gtcgacacgc tcgtggtgga
     1981 caagaccggc actctgaccg aggggaaacc ggcgctcgcg tcggtagcga cgcttccagg
     2041 ggtcaaagag tcggaactcc tgcggctggc ggcggggctg gaacgcggaa gcgaacaccc
     2101 gctcgcagcc gctatcgtcg ccggcgcgga ggctcgcgga ctgaacctcc cggctgtgga
     2161 ggggtttcgc tctctcaccg gacgcggagt tgctggggtg ctggaagggc gcgatgttgc
     2221 gcttggtaat cctcttttat tcagtgaatt agcgatagaa tctcgcgttt ttgacgaaac
     2281 ggccgagaag ttgcgcggac aagggcagac gatcgtctac gtggctgtgg acgggcgccc
     2341 ggcagggctg ctcggcgtcg ctgaccccgt caagaagtcg gcccgggagg ccatccagct
     2401 cctgcacaag gaagggatcc gggtcgtcat ggcgaccggc gataacccga caaccgcggg
     2461 ggtggtggcg cgggaactcg gcatcgacga cgtgcgcgcg ggggtcctgc cggaccaaaa
     2521 gagcaacgtc gtaaagcagc ttcaggcgga gggccgggtg gtcgcgatgg cgggagacgg
     2581 gatcaacgac gccccggcgc tcgcccaggc ccatgtcggc attgccatgg gaaccgggac
     2641 tgatgtcgcc atggaaagcg cgggtatcac gctggtgaaa ggcgacctgc gcggcgtggt
     2701 ccgcgcccgc aagctgagcc gtggcacgat gcggaacatc cggcagaacc tgttcttcgc
     2761 gttcgcgtac aacacgcttg gagtgccggt cgcggccggg gtgctctacc cgtttttcgg
     2821 cctgctgctc agtccgatga tcgccagcgc ggcgatgaca ttcagctcgg tttcagtgat
     2881 cagcaactcg cttcgtctgc ggagattgaa actctgacag gctgtctccg cccggtcacc
     2941 gcagaagccg ggcgacggac tcgcggagcg gggtgggcag tgtgcgcagg aggtccgcgt
     3001 caagatccgg gtggatggtg tggggcgaag tcacggacgc tgccgcgaca agcgcgccga
     3061 ggtggtgcgc ttcgacgagt ggcaaaccag ccgccagtcc cgcgatcact cccgccagat
     3121 gggcgtcgcc cgcgccggcc gtacccttta cggggacggc aggcactccg acatggcgga
     3181 gccgttcgcc gtcccacgtc cagcttccat cccgccccgc ggtgactagg agcgcgatct
     3241 gcggattgag cgcgcgcagg cgctcgaccg ccgccgcggc gatttcctcc ggctgacggc
     3301 ctgcactggc gagtcccgcc gccaccacgg cctcgtcgcg attgagggcg agcaggtcga
     3361 tgcgggagat cagcccggaa tcgatggctc ccggcaactc gacggaggtg aacgaggcga
     3421 cgcggaaaaa tccgtgcgcc gttcctaacc gcagcagcgt cgtgcgggct tcgatcggca
     3481 cttcgggggc cgccagagcc acgccgcaac cttcgtagcg ctgaaactcg ggctccaccc
     3541 gcagcacgta ggccgcgtca acgcgggagc aggccgagtc gttcgtggtc atgtttccgc
     3601 cgctgccgtc cggatacacg aaacagaacg agaacagcgt cggggcgtcc ggttccctga
     3661 tgacgtgccg gagatcaagc cctgcttcgg ccatctccac gagcaggcgc tctccgacgt
     3721 cgtcattacc gatcttgccg acggggatgg ttgcgaaatc ccggccgagc agccggctga
     3781 cgtagtggga gatgatatgc agcttgcagt agtcacgctg gttcaggaaa cgcccgccgc
     3841 ggctctcctc gcggccgagc gtctcattgc cgtcgagcgc gaagaagcgg ccggagccga
     3901 tgccgccgac gccgatcatg gcgcggtaga ggagtccgcc gggaggaatt cgaagggcgg
     3961 tcatgctgta cggtccgggg ccgaaaggcc gagatcccac tcggggtgtt cgctgacggg
     4021 gtgggacgcc gtcgcgatgg tcgcggtaat cagcgcgtcg gggtgttctt gtagcagcgt
     4081 gatcgggtac tcggtggtaa gaggaccgaa cagagccacg cggagcgcgg tttgtttcca
     4141 tgcgccagtg tcgctgaaga ggcgcacctt cttcgccgag agacactctt tgatgccgag
     4201 cgtgaccgac atcggcggaa cgaactggta ggcgctgccg aaggtgcgtt gagcgagggc
     4261 gagaatcgtg tcgaggttgt tgtcctggac ccgcacggtg gagttgcgca gatcgtcgag
     4321 cgtgatccgg ctgaacggat gccgacgcgt ctggttgtag gcgatgtggc cgtcctgtcc
     4381 ccaaccgcca taggtgatgt cgaccggcgc cgcccagatc ttccgccgca cctcctcgac
     4441 gttcccagcg ttcagccaga agcggtttga ttcgggaact gcaagcgcgg agtccaccgg
     4501 atcatagaaa tgcttctcca tgaagccgcg gaagctgtag ggatgggcgc ggggcaattc
     4561 gcggccctgc cagtcgagac actcgtccat gtggaatacg acgaagttct tcaggctgac
     4621 cccttcacga ttgacgagac tggtgaacgg ctggtaccag caggcgggac cgcacgggat
     4681 gatcgcccgt gtcgcctcgc ctcgggagtt gtgaaggcgg atctcgtcca ccaattcccg
     4741 ggccatgatg cggcccattt cttcggagtc ccggcagaga cggaactgca ccttgcgggc
     4801 gggatgggtt tcgagttgct ggtaggggat cctgcaccac tggtacaggt catagggtgt
     4861 gaagtccgcc atggctcttg agagaacaga gtatcagctt gaacggcgcg aggtggactg
     4921 cctctgaggg ctgcagcggc tgcaatcacg cgaagggcgc aggacgaaag acggaaagaa
     4981 tctcctcgat catcgcctcg cgggaagcgt aacgcgtctc gtcgtaacag ccgagcgggt
     5041 tctccagcac gttcactagg cacttgttgc agtaagtgca gggccggtcg ggccgatcct
     5101 tgcccgaggc gaagacgtgt gcgaggtcat tgttggcgat tagcggacgt gcgatggtga
     5161 cggcgtcgca cacgccgttc tctagggcct ggcgaatgaa ggaagccgtc tggaatccgc
     5221 cggtgcacag aacgggaatc ccgacagcct gcttgacggc gatcgcgtcc gggatgttga
     5281 tgccctcgat ggtttcaccg cgggccttga tccacacaga acgataaagc tgggcggccg
     5341 gaccgctgcg aaacgtcaga tagttgcgga gggctccgga gccgctcgaa agcatactgt
     5401 cgtaggtctt gaccgcttct tcgacaggga aatcgccagg cggattctta gggtgcggaa
     5461 aggcgcttcc ggaagaaacg tggatggcgt ccacgcccgc ttcttccagc cagcgacaga
     5521 cgttcaggga atccttgatg gtgttgccgg gtgattccca tggtacgagc gcgttgttga
     5581 actcggttgt gctgagtttc gcctggaggt ggaaatcccg cccgacctcg gcccggatgg
     5641 cggcgacgac ttccagaaga aagcgagcgc ggttgtgaag agagccacca tatttgtcct
     5701 tcctgtcgtt gatcgcggag cttaggaact gggtaaacag gtaaccgttg gcggcgtgca
     5761 actctactcc gtccagacct gcttcccgcg cccggcgcgc accggcagcg aatgcatcga
     5821 ccgtctggcg aatctgtgca agggtcatgc gctcgcactc aaagccgtga aaaggctccg
     5881 gcctgtcggt ggagctgagc cctttcgcgt actcgatgcc gccgatgtcg cgctggcggc
     5941 cggcgtggct caactgaagg atgaacttgc agtcatgctc gtgaacgcgc cggcccaact
     6001 cgcgccagaa cgggatgcgg tcgtcgcgat cgattgccgc atagttcggg atgatacggc
     6061 cgcgcatctg gaccgggaca aacgacgaaa tgatcgcacc aacacctccc cgcgcaaact
     6121 tcagttccca gttgatccgt gcggggttgc cggagccgtc atagttgtcg aaacggccgg
     6181 ccatgtttga tcgaaggatc cggttcttga cggtgagatt gcggaacgga agcggctgaa
     6241 agaggatatc ttccggcatg agcccagtgc ctcctctgaa cgagataggc ccatggtagc
     6301 aggtggctag cgcgtgcagc ccgcgaagac gttcagtacg gcaggcggcc gacacgcccc
     6361 ggcagaggga cgccgcggct tttctggctc ggctgtgggc cttcgttgcc ggtggaggcg
     6421 gtcggtcggg gcatcagggc accgctgaag ttgacttcct cgagggtgaa ggagatcggc
     6481 cggtcgagga ccatttcgat ggtggttccg cgggcgagga tggcgtcggg cccccgggac
     6541 aggagaacgc ccatgagacc ggcagtcgct ccggcggcag ctccgattcc ggcgcccatg
     6601 cccgcgtgtc ccgcaccggc tccagcgatt acgccgaccg acgctccggc ggcggtggcc
     6661 tcaccgacgc ttcgagcgtc gccgctcttg tttccttcgc tcttgatttt gccttccgtg
     6721 cggtccagtt cctcactgga gcggccgtca aggctcccta cacgggcgcg aaaatcgcgg
     6781 gtgacgccgt tcggcagggt cagcgaatcg aagcgcaggt acagctctcc ccggcccttc
     6841 acccggccgg gccgcttcac ctgggtaatg gttccggcga catagctccc cggcgggatc
     6901 accacacggc cgtcggcgag aatggggaag acggtctcca ggtacacacg gtcaccctcg
     6961 gcggagtgct tggtgctgac actgttgatc aggctcaacg ggatgcgggt gccggattgg
     7021 atgacgaggc tgcctggctc tggtgcttcg gccttcggcc agcccttttg tgtttcgccc
     7081 tccgccgccg gctgggtttg ccccaccagc gacagagtaa gcatggacaa caagatcaca
     7141 cagcggcccc acatagctgg ggatcctcct cttggcttta ttttagccca catagggtta
     7201 aacgcacggc cgcggagagt cgttgcggcg gttttctgac gcggccggta cgttcgtggc
     7261 acaattcccg ttatgctgac ggttcgacgg ttctttctcc tctctctgct gtttatcctg
     7321 atttcggccc ctccagcgct caccgccgac ttgtttcgtg acgatttttc gaaatttccc
     7381 gccggatggc tcacgcagcc gatcggtcag atcaacgcgg ccatccagga atatcactac
     7441 ttgccgcacc ggggtgtgcc gctggggctc tgggagaacg cgctctgtca catggatgcg
     7501 tggctgatca gcgaggagga tggcaaggcg tatctggaac aggccctcgt gaacgaccgg
     7561 ccacggcagg cgaacccgat cttcgtgaca ggcgatcccg agtggaccga ctacaccgtc
     7621 gaagcgaaag tgaagccgct gctccgttcc gacatggccg ggctggtttt ccggtatcac
     7681 accaatcggc actactacct gttcgccctc accgggggga accaagcgcg cctcgcggtg
     7741 cgcctcccga tcgagaagac cttccgcatc gcggaatggc gggaattggg ccgagccgaa
     7801 ttcaactatg acgcccgcgt gtaccacacg ctcaaggttg agaatgcggg gccgcggatc
     7861 agggcttcga tcgacggcaa ggtgctgatc gaagccgagg acggcgagat tctcaagggc
     7921 aaagcggggt tgacctcgaa catccccgcg cgctttcagg attttgtcgt caccgtaccg
     7981 gatgccgtgc gttccgggat cgatgaccgc atccggaagc gggaagccga actggcgcga
     8041 ctccgttcgg agaaccccag accgaagctg tggaagaagt tctcgacgcc ggtcttcgga
     8101 gccgggcgga acgcacgttt cggagatctt gacggcgacg ggaaactcga catgctgatt
     8161 gcgcagaatg tgcgccgctt cccgtacgac gccgcacacc tgacgtgtct cactgccgtc
     8221 aacttcgacg gcaaggttct ctggcagtcc ggcaagcccg acgcgcgcaa cgggctactc
     8281 acatacgaca acccgtttca aattcacgac atcgacggcg atgggcgcgc cgaggttgta
     8341 ctcgtcaagg atttcctgct ccaggtactc gacggccgaa cgggaaaact gattcaacga
     8401 gtgccgacgc cgcaggcccc agagggggac cgcaaccagg tttacgagaa tgtgagcggc
     8461 gattcgatcg ccttcgtcaa cgtcctcggc gacccgcggc ggcacgagat ccttttcaag
     8521 gatcgctacc ggaacttctg ggtctataac aacaagctgg agttgctctg gacaggctcg
     8581 gggcagacgg ggcactatcc ttacccattc gacgcggacg gcgacggtcg cgacgagatc
     8641 ctgatcggct acgccatgtg ggaccacgac ggcaagccac tctggagcca cgacaagacg
     8701 ctgagggatc acgccgacgg catcatgatg gccaacctca gtccggatcc gaaggccgaa
     8761 ccccgcgctt acgccggcgg aagcgacgaa ggcttcctga tgtttgacgt tcgcggcacg
     8821 ttattgaagc acgtgcgtct gggccacggc cagagcccgg tcgccggcaa gtttcggccc
     8881 gatgttccag gcttgcaatt cgctacggtg aacttctggt acaacccggg gatcgtcaca
     8941 atgttcgact gggacggaaa caccctggct caagaggaac ccattcattc gggaagcgtc
     9001 atgctgccgg tgaactggcg cggcgacgga caggagttca tcctgctttc cggcaattca
     9061 cgcgagggcg gcatgatcga cgggcattta cgccgtgtgg tgatgtttcc ggaagacggt
     9121 cacccggatc tcacggcgta tgtcgccgac gtgacgggcg accctcgcga cgaagtgatc
     9181 ctctggaacg aaaccgaagt ctggatctac acgcaggacc gcccgtttac cggtaagcgg
     9241 atctatgcgc ccatccgtaa tccagactac aacgagtcga actaccgcgt ggccgtttca
     9301 ctgcctcggt gggtggatcg gtgaggggaa ggaactccgc tccctgacgg tcgcggttcc
     9361 ttaacggcag tgcgggttag cataatggag tcatcttatg cgcaatcttc gagttgcatc
     9421 ggttcagttt gagtgcaagc cgggagacaa gaccgcgaac ctcgcaacga tccggcgctt
     9481 cgccaaagag gcagcccgcg cgggcgccga gatcctcgtg acgcccgagt gttccatcac
     9541 gggctactgg tttctgcgga atctcggacg cgaggagttt gccgcgctcg cagagccggt
     9601 cttcgacggt ccttcgtccc aggctctgat ggcaatgtcg aaggagtacg gcatgaccat
     9661 cggcgcgggg ttgatcgagc gcggcgatga cggcaagt
//
LOCUS       DATIFQ010000516         8676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2096302, whole genome shotgun sequence.
ACCESSION   DATIFQ010000516 DATIFQ010000000
VERSION     DATIFQ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096302"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..2193)
                     /locus_tag="VLE22_26000"
     CDS             complement(16..2193)
                     /locus_tag="VLE22_26000"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HSB17930.1"
                     /translation="MQSSPATRRHLALTLLVLAPVVVCAESRLNTQQLWKPSIDWGHW
                     RLGHRTDLDFLEKNRFTITFGSGAPNFETITRTEFDEGMEKARAFNRSYHDKGYIVLR
                     YLSSSLNGESDTGESQPRKDQLHVLKFFNERWQQFEDYVGPKPSESPMTWITIRPDGT
                     FPFYRYAPYGRKTGRGFETWGCPNNPDYVRMLEGRIRAQAETGIDGSYVDWTQIAGGT
                     CYCGYCRRNFVSYLSTNLPAKAARAKYGTADYSEIRLPEKRGDPFWMEWIAFRGHAVA
                     EFHKRLRSVARKYNPHFMISGNVFGGFGYGPIAYDAAGNIEMLGRDGYDDFVYSEIQE
                     FLDSAPRKNEQGVKITNSPALKYLAAATHGKPVIVYATEITPPVFPNPTEKCLSAMAQ
                     INIAEAAANHAVFREKRETPPGATRIHNFLAANEPSLLTAQLHSNVAVLASVNQYLAD
                     ELSFAFSTSRVLADRGIAHVMIVEDDPTRGGLSRFDLIVVPYLPLLSRPKQEALVRYV
                     EEGGSVLVLGKSGVKDEYNVPQATVPLARLFGASTFPGQALDRKRGKGRALFVPLPIP
                     ASRYLIPMKAKGEYTTFGPTMADVFADIPEGYTRNRIDPELRKILEGVAEKIVSSLPG
                     KLTRLTSSEPYVEITSMLSAGRERMLVHLVNYNVTLDGDVTPLRSLKVEAAVPAGRKV
                     KSIRYSGTLGDMKPLRFSSAAGSRVAFETSETGIYGLAVLELE"
     gene            complement(2217..3902)
                     /locus_tag="VLE22_26005"
     CDS             complement(2217..3902)
                     /locus_tag="VLE22_26005"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB17931.1"
                     /translation="MSGRRVGWYARTGLLLVVASAVWLPLSARRPETAAKRPHFTDVY
                     PRSKVSYFTNNNYTGRKYFQQPMCGGIAILDYDNDGKMDIYFTNGAKLPELKKDDPSF
                     YHCLLRNEGDGNFKDVTSTAGLSGSNLDFSYGVAAGDFDNDGHTDLFVCNTGKNTLYR
                     NRGDGSFVDVTDSSGLGQKPPDTLSVQAAWFDYDNDGLLDLVLSNYTLWTPEADKRCA
                     QGNVEVYCHPKTYVRVPHRLYHNLGGGRFEDVTDKSGFGKSLGKGMGIGIADFNDDGW
                     TDVFIANDTEPNFLYLNQGNGTFKEVGLLYGVAYNDTGATVSAMGCDVKDYDNDGWVD
                     IFYNNLMKQIWALFRNLHGKSFQYVSPASKILTLSGSRSGWSNGFIDYNNDGWKDLYS
                     ANGDVDNLGASSPQHDTMFENTDGKQFIDVSREMGDDFLRVGYQRGSAFADLNDDGFM
                     DLVVTSLNEKPRILFNSADNGQHWLLFKLTGAKSNRDAIGAKIKVTTPSGRTLYNHVT
                     VSVGFISSSDRRVHFGLGQETKAASVEIRWPSGIVQKLTDVAADRMVPVEEPR"
     gene            complement(3899..6226)
                     /locus_tag="VLE22_26010"
     CDS             complement(3899..6226)
                     /locus_tag="VLE22_26010"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB17932.1"
                     /translation="MLREASSVILLLGMAVVVPPAADAQPQDLREAARLDAEGKCDEA
                     ERYYRAALAKGTASSALLNNAGNHYIICGKPDKAHACFEKLLRLNPAHGNANLQLARL
                     AAEQKQGKKALEYLARVKETGPMVSLLRAEALHWAGQTSASRTMLDTVEKQAGGDPRL
                     LYVLGLTCGRLGLYDRAEAAFNGALADRPGDYDILFQLGRAAARAQHYDRAIRALETA
                     LKIRPGDVGVLLELGKVCAARQDFVRAVFFLAQAQRKAPGDADILLLMARAAEDAGYY
                     EDSAKAYDDYLRLRPSDDATRRDRARAYGHTDTRRDEARKELAWYLAKYPKDPLGHYV
                     YAQVFWHDEPQESLAHLTEAARLDPNSAAIRYSRGWMLQRNGQMAESLADLEAAKRLA
                     PESARIPDLIGMAHLALERHAEAEKAFRESLAKDPDDPEVVMHLGRTLVAAGREEEAQ
                     VYLEKYRKIRPPGRPMYRKQLGMIELATLPAREQRERAIGHLRREASAHPDHPEYQLH
                     LSSLLLADGRKEEALREFRVLLTLNADGKVREDAGSVLLSSGEYELAREFLQRAVEER
                     PAARLELTTALLYLDGPQAALQYLEKIPAEELTGDGLLLKASILEATGRRAEALTTLD
                     QGLSQGSSRPPVVQRAVLLLLRLDRKDDALSLLDRTIRSNPEDSGLPLTKAIVLGLVD
                     RVAEAEKTFKEVESRWPELDQAYLAHGLLLERSGRPREARQKLQTAVALGSEDSALRC
                     AQARLAGTPSSDPECACQTGLERLLFPGCAVESKQ"
     gene            complement(6337..>8676)
                     /locus_tag="VLE22_26015"
     CDS             complement(6337..>8676)
                     /locus_tag="VLE22_26015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB17933.1"
                     /translation="NTNNFVVSTPIKYPYKNFSDRADYIVNDKLRVYGRVSVTRTPVQ
                     VTGNPTGSPMFMSDRGADYNMTSYAGDATYALSPTTVLNFHGGYHNFRDTSHFATEFA
                     DEWSWEGVFPNSDFYKVVFADPGVPKLIPRMSICNQYNAPCTHMGPGGGLWHETPHAW
                     EFSGKLAQQRGSHYLKTGVDVRRNTTDSLILNVNPGFGFDGNPTSETYNNPNLLLSGD
                     PYATFLLGAIVPTDLASWGGNGNWWDSGATGFPVNIKPKVERRFYGMYLNDDWKVTRD
                     LTLNLGLRYEWESPFHDKQNRETRALDLDTAIPELQGVTMPDDVKQFYNGTWKMNGAF
                     RFADDEHPGAWDGGWGTLSPRIGAAYRLNDKTVLRGAYGRYVTPWTTNQNHDQLSGFP
                     LYGFSNFTGAPDAIQGVPQMQLNDPFPASNPIAPSYDKSLGIYTMLGNSLFYFNPDRR
                     RSNSDRINVSVQRQLPGSMVVDVTYFLNRTSQVFQTDYNINQVDPQIGYTYKEQTLAV
                     MDNPFFGILPVEKFPGQLRYQPQVGVTSMAKPYPQYGDITVVDGLKAGSMKYQALQIK
                     LQKNYSQGLSMLLGYSYHVEKDEEFYDDIANYQRKFSWQNPSLYRQRFTLAGTWDVPL
                     GKGRAYMNAAPRVVDAILGGWKLSPVLFWRSGNLLRFGGMLWDGSDPKVDNPTAQQWF
                     KTSGFERLPDFTPRTNPWDFPGLYGPGVLKLDGSLSKDFNITERVKAQLKADAFNLLN
                     NMSWGDPSTEFDDSHFGQSTNQAGLTFGRRVQLGARIEF"
BASE COUNT         1650 a         2594 c         2597 g         1835 t
ORIGIN      
        1 tcagagatga catcgtcact ccaactcgag gactgcgagc ccgtagatac ccgtctcgct
       61 ggtctcgaaa gcgacacgag atcccgcggc gctggagaac cggagcggct tcatgtcccc
      121 cagagttccg ctgtatcgta tgctcttcac tttcctgcct gcggggactg cagcctccac
      181 tttgaggctg cgtagcggcg tcacatcgcc atccagcgtg acgttgtaat tcaccaggtg
      241 taccagcatc ctctctcgtc cggcggagag catcgaggtg atctccacgt aaggctcgga
      301 gctcgtcagg cgcgtcagct ttccgggcag agacgagaca atcttctcag cgacgccctc
      361 gagaatcttc cgcagctcgg ggtcgatgcg atttcgtgtg tagccttcgg ggatatccgc
      421 gaatacgtcc gccattgtcg ggccgaaggt cgtgtactcg cccttcgcct tcatcgggat
      481 caagtaccgc gaggcgggga tgggaagcgg aacgaaaagc gcgcgccctt ttccgcgttt
      541 tctgtccagt gcctgaccgg gaaacgtgga cgcgccgaag agccgggcga ggggaaccgt
      601 cgcctgaggg acgttgtatt catccttgac gccggatttc ccaaggacga ggacgctgcc
      661 gccctcttcc acataccgaa ccagagcttc ctgcttgggc cggctgagga gcggaagata
      721 cgggaccaca atgaggtcga agcgcgagag gcctcctcgc gtggggtcgt cctccacgat
      781 catcacgtgc gcgatgccgc ggtcggccag cacccgcgag gtgctaaagg cgaagctcag
      841 ctcgtcggcg aggtactggt tcactgacgc aagaacggcg acgttgctgt gaagttgcgc
      901 ggtcagcagg ctcggctcat tcgcggccag gaaattgtga atccgtgtcg cgccgggagg
      961 agtctcgcgc ttctcccgga aaacggcgtg gttcgccgcc gcctcggcga tgttgatctg
     1021 cgccatcgcg ctcaggcact tctcggtcgg gtttgggaac accggcggcg taatctcggt
     1081 ggcatagacg atgaccggct tcccgtgcgt ggcggccgcg agatatttca gcgccgggct
     1141 attggtgatc ttgactccct gctcgttctt tcgtggtgcg ctgtcaagaa actcctggat
     1201 ctcggagtag acgaaatcgt cgtagccatc gcgccccagc atttcgatat tgcccgcggc
     1261 gtcgtacgcg atcgggccgt agccgaaccc gccgaagacg ttgccggaaa tcatgaagtg
     1321 ggggttgtac tttcgggcca cgctacgcag ccgcttgtga aactcggcta cggcatgtcc
     1381 tcgaaatgcg atccactcca tccagaaggg gtccccgcgc ttctccggga ggcggatctc
     1441 gctgtaatcc gccgtgccgt acttcgctcg tgcggctttc gcgggcaggt ttgttgagag
     1501 gtaggagacg aagttgcgcc ggcaatagcc gcaatagcag gttccgccgg cgatctgcgt
     1561 ccagtccaca taggaaccgt caatcccggt ctcggcctgg gcgcggatcc gcccctccag
     1621 catccggacg tagtcgggat tgtttggaca tccccatgtc tcgaaacccc gtccggtctt
     1681 ccgaccgtac ggcgcgtagc ggtagaaggg aaaggtcccg tcgggccgaa tcgtgatcca
     1741 agtcatgggc gattcggacg gcttgggacc gacgtagtcc tcgaactgct gccagcgctc
     1801 gttgaagaac ttgagcacat ggagttggtc cttccggggc tgcgactctc cggtgtccga
     1861 ctcgccgttg agggaactgg acaggtagcg aagaacgatg tagcccttat cgtgatagct
     1921 cctgttgaac gcccgggcct tctccatccc ctcgtcgaac tcagttctgg tgatggtctc
     1981 gaagttcggc gcgccggagc caaaggtgat ggtgaaccga ttcttctcga gaaaatcgag
     2041 gtccgtacgg tgcccgaggc gccagtggcc ccagtctatg cttggtttcc agagttgctg
     2101 ggtattcaac cggctctcgg cgcagacgac taccggagca agcacaagca gggtaagagc
     2161 gagatgacgg cgggttgccg gcgaagattg catcagacta tgctcctttg gcgttcttaa
     2221 cgcggttcct cgacagggac catgcggtcc gctgcaacat ccgtgagctt ctgtacgatt
     2281 ccgctcggcc accggatttc gacggaagcg gccttggtct cctgacccaa accgaaatgc
     2341 acgcggcgat cactagagga aatgaagccg acgctcacgg tgacgtgatt gtagagcgtc
     2401 cggcccgacg gcgtcgtgac cttgatcttc gccccaatgg cgtcgcgatt gctcttggcg
     2461 cccgtcagct tgaagagtag ccagtgctgg ccgttgtcgg ccgagttaaa cagaatccgg
     2521 ggcttctcgt tgagggaagt caccacgagg tccatgaatc catcgtcgtt caggtctgcg
     2581 aaggccgatc ccctctgata gccgacgcgc aagaagtcgt cgcccatttc gcggcttacg
     2641 tcgatgaact gcttaccgtc ggtgttctca aacatcgtat cgtgctgcgg ggaggacgcc
     2701 cccagattat caacatcgcc gttggcagag tacagatcct tccatccatc gttgttgtag
     2761 tcgatgaaac cgttgctcca gcccgaacgc gagccgctca gggtcaggat cttcgaagct
     2821 ggagagacat actgaaacga cttaccgtgg aggttccgga acaacgccca aatttgtttc
     2881 atcaggttgt tgtagaagat atccacccag ccgtcgttgt cgtaatcttt cacatcgcag
     2941 cccatcgccg acacggtagc ccccgtgtcg ttataagcga cgccgtacaa gaggcccacc
     3001 tctttgaatg tgccgttccc ctgattcagg tagagaaaat tgggttccgt gtcgttggcg
     3061 atgaacacgt ccgtccaccc gtcgtcgttg aagtcggcaa tgcctatacc catccctttc
     3121 ccgagcgact tgccgaaacc ggacttgtcc gtcacatcct cgaaccgtcc gccacccagg
     3181 ttgtggtaga gccggtgcgg gacacggacg taggtttttg ggtggcagta gacttcgacg
     3241 ttgccctggg cacaacgctt gtcggcttcg ggagtccaga gggtgtaatt ggaaagcacg
     3301 agatcgagaa gcccgtcgtt gtcgtaatcg aaccatgccg cctgcacgct caaagtgtcc
     3361 ggcggcttct ggcccaggcc ggaactatcc gtcacgtcga caaacgaacc gtctccccgg
     3421 ttgcgataga gcgtattctt cccggtgttg cagacgaaga gatctgtgtg gccatcgttg
     3481 tcgaaatccc cagcggctac tccgtagctg aaatccagat tcgagccgct gagcccggct
     3541 gtcgaagtca cgtccttgaa gttgccgtct ccctcattcc gaagcaggca gtgatagaaa
     3601 gacggatcgt ccttcttcaa ttccggcagc ttggctccat tggtgaagta gatgtccatc
     3661 tttccgtcgt tgtcgtagtc caggatggcg atgccgccgc acatcggctg ctggaagtat
     3721 ttccggcctg tgtagttgtt gtttgtgaaa taggagacct tcgaccgcgg gtagacgtcc
     3781 gtgaaatggg ggcgcttggc tgccgtttcg ggccgcctcg cggaaagcgg gagccagacg
     3841 gcggatgcca ccacgagaag caatcctgtg cgggcgtacc agcctacgcg ccggccgctc
     3901 actgttttga ctccaccgcg cacccgggga aaaggagccg ctccaatccg gtctggcaag
     3961 cgcactccgg atcggagcta ggggttccgg ccaatcgcgc ctgagcacat cgcaaagcgg
     4021 agtcctcgga ccccagcgcg acagccgtct gaagcttctg gcgagcctcg cgaggcctgc
     4081 cggatcgttc caacagcaag ccgtgagcga ggtaagcctg atccagctcc ggccaacgcg
     4141 actcgacttc cttgaatgtt ttctccgctt cagcgactcg atccaccagg cccagtacaa
     4201 tggctttcgt aagggggagg cctgagtcct cgggattcga ccggatcgtc cgatcgagca
     4261 ggctcagcgc atcgtccttt ctgtcgagtc gcagcagcaa cagaacggcc cgttgcacaa
     4321 ccggcggtcg cgaggaccct tggctgagcc cctggtcgag tgtcgtcagc gcttcggccc
     4381 tgcgtcccgt tgcctcgaga atgctggctt tcaggagcaa cccgtccccc gtcagttctt
     4441 ctgccgggat cttctcgagg tattgcagcg ccgcctgggg tccgtccagg taaaggaggg
     4501 cggtcgtcag ttccaggcgc gccgcgggcc tttcttcgac ggcgcgctgt agaaattccc
     4561 gcgccagttc gtattccccc gaactgagga gcacggatcc tgcatcttcc cggaccttgc
     4621 cgtcggcgtt gagcgtcaac agaacacgaa attcccgaag ggcttcttcc ttccggccgt
     4681 ctgcaagcag gaggcttgac agatgaagct gatactccgg atggtccgga tgggcgctgg
     4741 cctcgcggcg gagatggccg atcgcgcgct ccctctgctc tcgggccggc agagtcgcca
     4801 gctcaatcat tcccagttgc ttccggtaca tgggacgtcc tggcgggcgg atcttccggt
     4861 acttctcgag ataaacctgg gcctcctctt cgcgtcccgc cgccaccagg gtgcggccca
     4921 aatgcatcac gacctccgga tcgtcggggt ccttggccag agactcgcga aaggccttct
     4981 ccgcttcggc gtgccgttcc agggcaaggt gagccatgcc gatcaggtca ggaatgcgag
     5041 cgctttccgg agctaggcgc ttggcggcct ccagatccgc cagcgactcg gccatttgcc
     5101 cattccgctg gagcatccaa ccccgagagt agcgaatcgc ggcagagttg gggtcaaggc
     5161 gggcagcttc ggtcagatgg gcgagagatt cctgaggctc gtcgtgccag aagacctggg
     5221 cgtagacgta atgccctaag ggatccttgg gatatttcgc cagataccag gcaagttcct
     5281 tgcgggcctc gtcccgccgt gtatcggtat gtccgtacgc ccgggcccga tctctccggg
     5341 tggcatcatc gcttggccgg agccgcagat agtcgtcgta ggctttcgcg gaatcttcgt
     5401 aatagccggc atcctccgcc gcccgggcca tcagcaggag aatgtcggcg tctccgggag
     5461 cttttcgctg cgcttgtgcc agaaagaaaa cagcacgcac aaagtcctgg cgtgcggcac
     5521 aaaccttccc tagttccagc agcacgccga cgtcgcccgg acggatttta agcgcggtct
     5581 ccaaggcccg gatggctcga tcgtagtgtt gtgcgcgggc agcggcccgg ccaagttgaa
     5641 agagaatgtc atagtcgccg ggccgatcgg ccagcgcgcc attgaatgcc gcctccgccc
     5701 gatcgtacag gccgagccgg ccgcaggtga ggccaagtac ataaagcagt cgcgggtcgc
     5761 cccctgcctg tttttcgacg gtgtcgagca tcgtgcggga agcggaagtc tgtccggccc
     5821 agtgcaatgc ctcagcccgc agcagcgaga ccatcgggcc ggtttccttc actcgtgcaa
     5881 ggtattccag cgccttcttc ccttgttttt gttccgctgc aagacgggcc aactgcaagt
     5941 tggcattgcc atgggcgggg tttaatcgca agagcttttc gaaacaggcg tgggctttgt
     6001 ccggcttgcc gcaaattatg tagtggttgc cggcgttgtt cagaagggcg gaagaggcgg
     6061 tacccttcgc cagcgcggcc cgatagtaac gttcggcctc atcacacttt ccttccgcgt
     6121 ccagacgtgc tgcctcacgc aggtcctggg gctgtgcatc cgctgcgggg ggaacgacaa
     6181 ccgccatccc cagcaacaaa atcacgctgc tggcttccct gagcattcgg actcaatctt
     6241 atctggatct gcgaaggtgc tgcaaaccac aaaaaagccc cctccgcgcc ggtaggggcg
     6301 agagggggct tcgagactcc agactcaaca aaggctctag aactcgatgc gagctcccag
     6361 ttggacgcgc cgtccgaagg tcaaacccgc ctggttggtg ctttggccaa aatggctgtc
     6421 gtcgaactcg gtggacgggt caccccagct catgttgttg agtagattga aggcgtctgc
     6481 tttcaactgt gccttgactc tctccgtgat attgaagtcc ttcgaaaggg aaccatccaa
     6541 tttgagaacc cccggcccgt aaagaccagg gaagtcccat ggattggtgc gaggtgtgaa
     6601 gtccggcagc ctctcgaacc ctgaggtctt gaaccactgc tgagcagttg ggttatccac
     6661 tttggggtcg gagccgtccc acagcatccc gccgaatctc agcaagttgc cggaacgcca
     6721 gaacagcacc gggctcaatt tccatccgcc cagtatggcg tccaccaccc gtggcgcagc
     6781 gttcatgtag gctcgccctt tcccaagcgg aacgtcccat gtgcctgcca aggtgaaccg
     6841 ctggcggtac aggctcgggt tctgccagct gaacttgcgc tgataattgg cgatgtcgtc
     6901 gtagaactcc tcatctttct cgacatggta gctgtagccc agcagcatcg acagcccttg
     6961 gctgtaattc ttctgtagtt tgatctgtaa cgcctgatac ttcatggagc cggctttgag
     7021 cccatccacg acagtaatat cgccgtattg tggataaggc ttcgccatgc tggtcacgcc
     7081 cacttgcggc tgatagcgga gctgtccagg aaacttctcg accggcaaaa tgccaaagaa
     7141 ggggttatcc atcaccgcaa gcgtctgttc cttgtaggtg tagccaatct gtggatccac
     7201 ctggttgatg ttgtagtctg tctggaacac ctgactggtc cggttcagaa agtaggtgac
     7261 atccaccacc atcgaacccg gcagttgacg ttgtacggag acgttgattc ggtcgctgtt
     7321 gctacgcctc cggtcggggt tgaaatagaa cagggaattc cccaacatgg tgtagatgcc
     7381 gagactcttg tcgtacgagg gagcaatggg gttggaagca gggaacgggt cgttcaactg
     7441 catctgcggt acgccctgaa tggcgtcggg agcgccggtg aaattgctga aaccatacag
     7501 ggggaaaccg ctaagttggt cgtggttctg attggtggtc cacggggtta cataacgtcc
     7561 gtaagccccc cgcaggacag ttttgtcatt caaccgataa gcggctccga ttcgcggtga
     7621 aagtgtcccc cagccgccgt cccaagctcc gggatgttca tcgtcggcaa accggaaggc
     7681 cccgttcatt ttccaggtcc cgttgtagaa ctgcttcaca tcgtccggca tcgtgacgcc
     7741 ctgcaactcc ggtatcgccg tatccaaatc cagggcccgc gtctcccggt tctgcttatc
     7801 gtggaacggt gactcccact cataccgcaa gccaaggttc aaggtcagat cccgcgtcac
     7861 tttccagtcg tcattcaggt acatgccata gaaacgccgt tccaccttcg gcttgatatt
     7921 gaccggaaag ccggtggcgc cgctatccca ccagtttcca ttgccgcccc aagaggccag
     7981 gtcggtcgga acaatggctc ccaaaaggaa cgtcgcatag gggtcgccgg acaacaggag
     8041 gttcgggttg ttgtaggttt ccgaagtggg attcccgtca aagccgaagc ccggattgac
     8101 gttcagaatc agactgtcgg tcgtgttgcg ccgaacgtcc acacccgtct tgaggtagtg
     8161 cgagccgcgc tgctgggcga gctttccgct aaattcccac gcgtgagggg tttcgtgcca
     8221 taagccgccc cctggcccca tgtgggtgca tggcgcgttg tactgattgc agatgctcat
     8281 gcgcgggatc agtttcggga cgcccggatc cgcgaacacg actttgtaga agtcactatt
     8341 cggaaagacc ccttcccagg accattcgtc ggcaaactcc gtcgcgaaat gggaagtgtc
     8401 cctgaaattg tgatagccgc catggaagtt gaggaccgtg gtggggctca acgcgtacgt
     8461 ggcatcgccg gcgtacgagg tcatgttgta atccgcgccc cggtccgaca tgaacatcgg
     8521 cgaaccggtg ggattaccgg tcacctgaac cggcgtgcgc gtcacactga ccctgccgta
     8581 cacccgcagt ttgtcgttga cgatatagtc cgcccgatcg gagaagttct tgtacgggta
     8641 cttgatcgga gtcgacacca cgaagttgtt ggtgtt
//
LOCUS       DATIFQ010000517        24661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2096660, whole genome shotgun sequence.
ACCESSION   DATIFQ010000517 DATIFQ010000000
VERSION     DATIFQ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24661
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096660"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VLE22_26020"
     CDS             <1..496
                     /locus_tag="VLE22_26020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17934.1"
                     /translation="SPPSPPPPPARRSEPPRAGAVSRTTQPPTTPATAKPTSPTLGDS
                     GWKQKLHAAFVELGMPFTADAIEHSDVTEVAGELQFLTPKDFRLSMKEPDLRRALTHL
                     GFASMRLKVTFGEPTAPAPVAPQEPATGDENELTRRALAHPEVQRFRETFGGQVRTVR
                     NLKE"
     gene            586..894
                     /locus_tag="VLE22_26025"
     CDS             586..894
                     /locus_tag="VLE22_26025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HSB17935.1"
                     /translation="MKLPGNLQAMMKQAQQMQERLQEEIAQVRVEASAGGGMVTVQMS
                     GQKNVLGVRIDPEVAGDVEMLQDMVMAACNEAAKKVDEAIQQKTSGLLGGLGLPPGMF
                     "
     gene            910..1509
                     /gene="recR"
                     /locus_tag="VLE22_26030"
     CDS             910..1509
                     /gene="recR"
                     /locus_tag="VLE22_26030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HSB17936.1"
                     /translation="MPDFAEPLARLVLEFKRLPGIGQKSAQRIAFHVLRGSRDDAARL
                     AQAVLDVKDKLSLCAVCNNVSDGEMCPFCLDPGRDGSVICVVEEPHNIMPVETTRQYQ
                     GLYHVLHGAISPLRGVGPDQLRIKSLLERLEGSPVKEIILATNPTVEGEATASYLARL
                     LKPLGLKVTRIAMGIPVGSDLDFADEVTMTKSLENRREL"
     gene            complement(1513..2817)
                     /gene="mtaB"
                     /locus_tag="VLE22_26035"
     CDS             complement(1513..2817)
                     /gene="mtaB"
                     /locus_tag="VLE22_26035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HSB17937.1"
                     /translation="MGTFFIQNFGCRATQADGAALETALERDGFETARECRQADLVIL
                     NTCTVTASADEDVRQTVRRVRRENPAARILVTGCYAQRAPEELAAMPGVTWVVGNSHK
                     VRIPEILREEGATTPYHGQIFVGDIFAQQEFLAAPVEDAAGDRTRPNLKIQDGCSNRC
                     SFCIIPSVRGRSRSAQPDEVVERVRRLSLRYREVVLSGINLGRWGREAGIQMRLADLV
                     RRLLEETAIERLRLSSIEPMDLSDDLLGLMASSPRIAGHVHAPLQTGSDSVLRRMRRK
                     YRPRHYEDRIRKARALMPDAAIGADVMVGFPGETDEEFEENRSFIDSLPFTYLHVFTY
                     SGRPSTPAAEMTGQVPIPVRKHRNRILRELAVDKNLEFRRRMVGKRLSVVTLEQEGTA
                     LSDNYLKVEVAVPREPNQLIEVAIGGVTPSGLREKDPFHILF"
     gene            complement(2867..3922)
                     /locus_tag="VLE22_26040"
     CDS             complement(2867..3922)
                     /locus_tag="VLE22_26040"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSB17938.1"
                     /translation="MRIAGVPVRFHFTFLLLLVFLVVVGAGSGSTGWSYVLYVLALFG
                     SVLLHELGHAIVSRRYGIRTLEIVMFPIGGVARLERNPRPREELWISIAGPAVNLVIA
                     GAIVGTLAQLGGLPSLASLEDPAAATLWVKIAGGNLVLAIFNLLPAFPMDGGRILRSL
                     LALRRPEEEATRIAAAAGRFLAIALGLYGLLSMNFLLVFIAFFVYLGAAQEGTLAVGR
                     LYMQGAPVRSAMVTDFRTLSHGDTIRDAANLLLATSQQDFPVVHGEQVVGMLSRTALL
                     KALASEGPEAYVAGAMDRDFVRVSPNIDLVEALPLLGQSKACALVMEGDRLVGLLTNE
                     NLAEYILVRRFGLRPGK"
     gene            4111..5325
                     /locus_tag="VLE22_26045"
     CDS             4111..5325
                     /locus_tag="VLE22_26045"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HSB17939.1"
                     /translation="MIKRIIAIAFIFFCAAVAWAVLGATIFSRTYDSDLRLKGRVVSS
                     WGAPHVQRPPRACYDRRVPKTVETVEDGRKVLRTTQETVSTVLPLEGSRVRAGLNLEH
                     RQKGLLWYSTYRVVFGGTYTFRNTSDAEQQVRFILDFPAEQAIYDDLTFAVGGVPVPF
                     DGGEKSASATAPVPPGKTVQLTVGYRSQGVESWRYNFGADVAQVKDFSLRMTTDFKDI
                     DFPENALSPSEKRETQDGWDLAWNYKNLVTGFNIGMIMPEKLQPGPLAGRISFFAPVS
                     LFFFFFLMLVITTLRGIDLHPVNYFFLAGAFFSFHLLLAYLVDHVSIHAAFVICSAVS
                     IFLVVSYLRLVVGIRFAAVEAGLAQLIYLVLFSYAFFFKGFTGLAITIGSILTLFVVM
                     QITGRIRWAEKF"
     gene            complement(5362..9123)
                     /gene="smc"
                     /locus_tag="VLE22_26050"
     CDS             complement(5362..9123)
                     /gene="smc"
                     /locus_tag="VLE22_26050"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HSB17940.1"
                     /translation="MLKLKRLELQGFKSFCERTELRFNGSGIAAIVGPNGCGKSNLSD
                     AISWVLGEQSAKSLRGTRMEDVIFAGTRDRKPLGMASVTMTLVDPSVHVEHSAAHAKS
                     NGKATNGSGTNGTGAVEAAAHGAEANGANGAAKERPAEITITRRLYRSGESDYLIDGR
                     LARLRDIQDIFMGTGLGPESYAILEQGRIGQILSSRPQDRRSVVEEAAGITKFKTKRR
                     LAEAKLEGARQNLARVFDILEEVGRQVNSLKRQASKARRYEELKTEMDAQLRRLLSGR
                     YRMLERDAAKTALDLNVAVTEFQRISGDVASRQEEYSRLQKETYRIENELTGARRRLA
                     DLQLEAERTRGQLANQAQQIASIEERLSQGEAEAEQLSGRATALQLELDAQLARIAEL
                     EQQLDSSRERLSAKSTEREKLQDSLRGREQQIEATRQSVLRLLGESSTLKNELAQVEG
                     YLASLERDTARARKEEQSALTDLEWLETSRKELSEKAAARQLELESTVEQRRRAEEAL
                     ADRRTRAAETRQQLDRLRSEFSGIKARRDSLQEILSHRAYTTESVKRLFTAIDQGQAN
                     DLKPLGVLADFVDVDAAFEKAAEEFLHDELEYVVVESWNQAERGIDLLRSDLEGRATF
                     LVHPKGDADGGDLRHEEPAIGPETGIISRLSDVLKLTNGLTHAPVELLPRLARCFLAE
                     DRVAAQRLASQYPGLYFLLPDGICYSSQAVSGGKKTGSGPLALKRELRELSSQFHSRQ
                     TELEAVRVALESLEREVSVLEQELERLRTEQQGQEKEALALDHEMRKLAEELTRANSR
                     LSVAHLELGRLGQEKERSEAKREEVGRFLEEREEARRQQEFSLEAARQELEQLQTAAA
                     GIGEEHSALRAELAGQEERARAERDSKTRIEGQLNEIAVRRQEIAGETERLGVERARL
                     LANNIELDQRAASLSGEISDAESGVAQLTESETGARASLAGLEESLKSLRAAAQEAQE
                     RRSQIELELVKRQSELKFLDETSRKDLNVPIQELAQPEEAEIDELALAETEEKFQQVK
                     SRIEALGPVNPQALEEYEEARQRYDFLNTQRQDLLDSIRDTEKAIHDIDVETRRRFAE
                     AFEAINANFREMFKTLFGGGIGEMRLTDESNVGESGIDIVASPPGKKLQNVLLLSGGE
                     KALTALALLMAIFRYQPSPFCILDEVDAPLDEANIQRLTRLLKEMSEQTQFIIITHAK
                     RTMEVAQALYGVTMQEPGVSRLVSVRFQPLAPPLPMVAPVAAGLQGAPN"
     gene            complement(9283..11277)
                     /locus_tag="VLE22_26055"
     CDS             complement(9283..11277)
                     /locus_tag="VLE22_26055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HSB17941.1"
                     /translation="MFRRYHVPMEKTILALLFLLPNMEAAETPSGWTPELAMEIQTVG
                     SAVPSPDGRLVAWTQTRAVMEAEKSEMISQIYLGRSDDSWRVQLTRGEKSSSAPSFSP
                     DGSYVYFQSARSGKQNLWRISVAGGEAEILTDWKGSIGAYRVSPDGKWIAFAGVPDDP
                     EEEKSKKEKRDFRVVDENPKNHGLWVIAAEPGASGTTSPRKLFANAYHITDLDWAPDS
                     RKIAFEHQPTPNADEWRLTDISEVDVESGTVRPLAVTGAAERTPLYSPDGRYLAYTVS
                     TEPVRWGGDARVALLPRQGGQLRVLPASYDERPVFAGWSGDSSVLYFAENKQTRTALY
                     AMPVDGVPKLVYEPQQGVAGPSVRLNAKGTHAGFTCESSLEPPEACVLSLSATSPVRV
                     SRANTDKVLPSIGETKLVRWKAKDGLDIEGLLTYPVGFESGKKYPLILNIHGGPMGVF
                     NESYPGRPGLYPLAAFASKGYAVLRCNIRGSGGYGKQFRFANYNDWGGKDYDDLMAGI
                     DHVIGMGVADPGRLAVMGWSYGGFMTSWVIGHTRRFKAAAIGAPVTNLWSFTGTSDIP
                     GFLPDFFAGEPWDSFEAYRKHSPMSYVKGITTPALILHGEGDIRVPISQGYEFYNALK
                     RQGIPAKMVVYPREPHGPQEPKFMLDIMRRHLDWVEKYIR"
     gene            11336..11866
                     /locus_tag="VLE22_26060"
     CDS             11336..11866
                     /locus_tag="VLE22_26060"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HSB17942.1"
                     /translation="MSQSIVLADVSRSLARFLRKELQADPAVGEWMDSDERISLDSPH
                     ALQEDGAVRLSLYLYHVFTDQTPRTRVPMRVEPGPRHEPPLVVNLLYLVTPVTGTPVE
                     QQTLLAAAMRALHRKPLVEGTDLFGAAASLGEPVRLTASPLTLEETARLWQSLGMSHR
                     PSVCYAVRLVLPQSES"
     gene            complement(11888..14155)
                     /locus_tag="VLE22_26065"
     CDS             complement(11888..14155)
                     /locus_tag="VLE22_26065"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HSB17943.1"
                     /translation="MPDIVPRDLALVYNAAMLRGLLILLLLAPFAGAADIQIRGLKEP
                     VEVLRDRWGVPHIYARNTEDLFFTQGYLAARDRLWQIDLWRRVGTGKLAEALGPTALP
                     RDGLAWAVRYRGNWSAEWESYGPGARDIAVSFTSGINAYIRELGGKRLLEFRIAGYDP
                     GLWEPEDCLARMAGILMTRNVQREVRRVEDARQFGLKTLQHFLPPDPFIPLRIPNGLD
                     LDDVTAEVLRVYNEAVGPVKFSQEQGSNNWVVDGTMTATGKPILANDPHRPLQIPSLR
                     RTVHLVAPGWSLIGAGEPALPGIALGHNGKIAWGFTIVGIDQQDLYVERLHPSDPNRY
                     LYRGEWRAIEVERVRIPVRGETESAAVELKHTIHGPIIQEDPQRRRAYALRWVGSEPG
                     TAGYFAGLAVARASTWNEFLDAMERWKVPSENIVYADAAGNIGWQAAGLTPIRTNWSG
                     LFPVPGHTGEYEWSGFRRVAELPRKFNPPSHFIATANHNILPSDYRIPIGYEWALPYR
                     YERILELISGGNKFSVADFQRFQQDIVSVPARRFQKVLQKWNPPTGSEAGEIREQLLR
                     WDAAMAVDSVPAAIYAVWISKLPAELFGPALGARTDLAMLLKTLEARSEPNALQRSLG
                     AALKELQSRLGPDRKQWRWGRLHQLHFRHDLNRAAFHCGPFERPGDAQTVNAASGTDF
                     RQANGASYRQIIDLGDWDRSVMTNVPGESGNPENPRYSDQLDGWLAGRYHPMLFSRKA
                     VEAATIERMTLVPEE"
     gene            14189..15148
                     /locus_tag="VLE22_26070"
     CDS             14189..15148
                     /locus_tag="VLE22_26070"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HSB17944.1"
                     /translation="MRQLLPLLFAPFVLQAHMVSLSTGEIRIEGQRGYYELRMPLYEI
                     AHVRDPERTLFEHIRFASGGSPARQVSRSCREDAEAGAYICRAEYVFPEPAVILEIEC
                     TLHAVTVPNHVHLLRAVNGGRTDQAVLDFSFSKTTLRFRPPTTFETATTHLSAGMMRA
                     AGGAAQLLFLASLVLAARRRRELLALTGMFLAGEVASCLLLPITGWQPAPNFIEAAAA
                     LTIAYLAVEILLLPEAGMRWLVVGVLGAFHGLYFEIFIRNSGYSALWVLTGVVLAEVL
                     LIAVFALIFSRIAKATEALHPVKVSASVLLVVGVAWFVLRLRG"
     gene            complement(15150..16838)
                     /locus_tag="VLE22_26075"
     CDS             complement(15150..16838)
                     /locus_tag="VLE22_26075"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSB17945.1"
                     /translation="MQANRRDFLRQSGGLLLLPGVLAGAREFRCEVAVIGGGTGGCAA
                     ALAALRDGMRVILTEETDWVGGQLTAQAVPPDEHQWIESFGCTRLFRTYRDAVRQYYR
                     DHYPLTAVARGRRYLNPGDGGVSRLTHEPRVSLAVLESMLAPYLGGGQLVVLLRHKPV
                     SADVDKDSVRSVTVKGVESGRERTVYAAYFVDATEQGDLLPLTRTEYVTGFESRKQTG
                     EPHAPEQPQPANMQAFTVCFAVDHIEGEDHTIDKPDEYSFWRDYVPQLRPPWPGKLLS
                     WSMSNPVTLELRPVMFDPELKAPAEQRLNLWLYRRIANRRNFAEGAYRSDISLINWPQ
                     NDYWLGNLHEVSEDEAAGHLLRGKQLSLSLLYWMQTEAPRPDGGHGFRGLRLRHDITG
                     TEDGLAKYPYIRESRRIQAEFTVLEQHVGTDARMKLTGRKREELTAEVFGDSVGVGSY
                     RIDLHPSSGGDNYIDVSSLPFQIPLGALIPKRVENLLPASKNLGVTHITNGCYRLHPV
                     EWNIGESAGAVAAHAIHTKNPPRRIRNSEKLLKEFQTRIVEQGIEVEWPKLSPR"
     gene            complement(16849..17715)
                     /locus_tag="VLE22_26080"
     CDS             complement(16849..17715)
                     /locus_tag="VLE22_26080"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSB17946.1"
                     /translation="MSVLGVDIGGTKIAAGTVNDGACVVHGVTVPTRAAEGYDVSIGQ
                     VYKAIGECLPAGVRAIGICAPGPLNPKTGVILNPPNLPGWRNVPLANLVGERFGLLCR
                     VENDANAAGLAEARFGAARGFDSVLYVTLSTGIGAGIILDGKVYHGKNGAAAEGGHVT
                     IDWRSGTICNCGTPGCIEALASGSAIAKRGRTPEQIADGVARGDGESLRILDEIASML
                     GAWMGSMISLLDPDIIVVGGGVSRIGEPLFSRLRRIAPSRTINQFAAGTPIMPAELAE
                     NVGIIGAASVVL"
     gene            17782..18735
                     /locus_tag="VLE22_26085"
     CDS             17782..18735
                     /locus_tag="VLE22_26085"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HSB17947.1"
                     /translation="MGLITISGQPGCRHDEVARLTAQRLGFELVTETRLHSLIEREFG
                     PATPISDKAYRHVLLSILARLATEHHLVVSAFGVEHVFQNFLGVLRVYVVAPEFRRIG
                     TLMLDRRLDRPSAKRSLRQMEREESEERRRKFGRATVPSHAFDVVLNSEFLESDQLTD
                     LIEAAARARSLVEQGLLSSAADAQLQFQARLQLSRYGIMPADAVSLKRSNFSHPSEEI
                     FANLLDFYRVAWEYEPRSFPVKWDKDGRVLEAFTPDFYLPEFDLYVELTTMKQPLVTK
                     KNRKVKLLREIYPEINIQVFYQKDFQNLIFKYGLTERLARV"
     gene            18732..19271
                     /gene="hpt"
                     /locus_tag="VLE22_26090"
     CDS             18732..19271
                     /gene="hpt"
                     /locus_tag="VLE22_26090"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HSB17948.1"
                     /translation="MSKAQPPGIERILFTEEQIQQRIREVAGEISERFRGRDLKLIGV
                     LKGSIFFLTALAREIEIPIKIDFLGISSFSNRTGAPGVVRIAKDLDESIEGEDVLLVE
                     DIVDTGFTARYLLQTLAGRGPNSLGICTLLDRTSSRIVQVSVEFRCFEIPDRFVVGFG
                     LDYKQFYRNLGFVAVMKRD"
     gene            19440..19838
                     /locus_tag="VLE22_26095"
     CDS             19440..19838
                     /locus_tag="VLE22_26095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17949.1"
                     /translation="MQQGVLFISSSAEDANALTGMLGPLSIPLKHATDLRFARTALDS
                     EKYGVILTEARLADGNWEDVINIVEKQDPSCAVVVTHPLADARFWADAIETGAYDVLA
                     QPFCCSEVQRILSNALSSPVELKRLSRAAI"
     gene            19864..21240
                     /locus_tag="VLE22_26100"
     CDS             19864..21240
                     /locus_tag="VLE22_26100"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HSB17950.1"
                     /translation="MSQPAHQFIEQNRERFLEELKEFIRIPSISTLPENQGDIQRAAR
                     FVGERLTGAGMENVEVIPTGSHPLVYADWLHAPGKPTVLCYGHYDVQPPDPLEEWVSP
                     PFEPTLRDGNLYARGAVDDKGQLYAHIKAVEALRAVNGSLPVNLKFLIEGEEEVGGAS
                     ISKYVEQNPEKLKADVALVSDTALYAEGMPTLCIGLRGLVYMEIEATGPSRDLHSGLY
                     GGAAPNPIFGLVELLSKAKNRRGVIQIPGIYDDVEPPAPAELESWTSLPFNEKEFLKK
                     EVGASKLTGEPGRSVLERVWARPTLEVHGIAGGFTGAGAKTVIPAKATAKVSLRLVPR
                     QDPEKVIAAVREFVRKKTPKGIRTEVRVLSAAPGVLVNPDHPAVHVAAKVFGEVFGRP
                     TVFIRSGGSIPIVGEFAKHLGIPTVLMGFGLPDDGLHSPNEKFRLENFYLGIKTVSSF
                     LEEYGQNR"
     gene            21158..22879
                     /gene="murJ"
                     /locus_tag="VLE22_26105"
     CDS             21158..22879
                     /gene="murJ"
                     /locus_tag="VLE22_26105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HSB17951.1"
                     /translation="MRSFGLRTSIWVSRPSRASWKSTGRTGSVLAQQHEKIVRSAGVV
                     SASVFASRVSGLVREIVMARLFGAGQLYDAFLLGFRIPNLTRDLFAEGALSSAFVPTF
                     TQYLNTKSRREAAVLANLVTTAVLIVVGALCTLGMIFSPELVRLLAPGFAAVPGKFEL
                     AVQLTRIMFPFLLLVALAAQAMGMLNACGQFGVPALSSTFFNIGSVLFGLVAGFWLGP
                     RLGFEPITGMAIGVVLGGGLQLLWQVPSLRRSGFTVRLLTDWSHPGLQQILRLMGPAI
                     LGNAAVQINVMVNTNFASGIHDPVTGADGPVSWLGYAFRFMQLPLGLFGVAIASATLP
                     AISQSASLGRFDEFRQTLSRSLGMVFLLTLPSSVGLAVLGESMIGAIYEGGKFRHYDT
                     QQTALALSFYAVGLGGYAALKVLTPAFYALHDARTPMFISLASILINYIVAAALVRSG
                     TLGHAGLAMATSAVAWFGFVALFSIIRGRIGGVYGRRLFESVLRISAASIAMGVAVVL
                     SSRLLESWLGGSRLGRLADLAISIPIGVFVFYGACRVLRVAELELAGRALAAPLSAWL
                     SRQRGKI"
     gene            23000..23644
                     /locus_tag="VLE22_26110"
     CDS             23000..23644
                     /locus_tag="VLE22_26110"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HSB17952.1"
                     /translation="MESHIRKLEADQAALAANQRERKKLDDDIKVQNQKTSKLKDQMM
                     EAKTNEQYRAFQHEIDYGMKEIRKAEDRILDLMAESEPLEQNLRTAELALKDEKVRVE
                     SEKKAARERTAKDKQELDVLSTERRQLVAELTPQVYSAYDRIRKKRRGVAVAEAVDGR
                     CGACHMALRPQFFQDLRASEQVMFCESCGRILYYNPVIVVEPDMSAEAGTEPPQ"
     gene            23768..>24661
                     /locus_tag="VLE22_26115"
     CDS             23768..>24661
                     /locus_tag="VLE22_26115"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSB17953.1"
                     /translation="MKVYEGKDIRNVGLIGHGHSGKTTLAAGALFLTGATNRLTSVDE
                     GNTLTDFDDEEIQRKISISTAIACLEWKKQKINVLDTPGFNTFINDTQAALTAADAAL
                     VLVDGVAGVEVTTEKVWSFAKTYGQPRALVINKLDRENSSFARVLESIHENFGRTAVP
                     IQLPIGSEREFRGAIDLVRMRAFMYGPEPSGKGNEEDIPATMKEAAEKARETLVEMVA
                     EGNDALMEEFFEKGTLAPEQIQEGLRAAVKDMRLFPVLCASTSKNLGTDLILDFVVDY
                     FPAPTERGPLAGVVDGAETTRS"
BASE COUNT         4491 a         7941 c         7342 g         4887 t
ORIGIN      
        1 gtctccgccg tctccgccgc ctccaccggc ccgccgctcc gagccgccgc gtgccggagc
       61 ggtatcgcgt acaacccagc cgccaaccac gcccgcaaca gcgaagccca cctctccaac
      121 actaggcgac agcggatgga aacagaaact gcatgccgcg tttgtcgagc tcggcatgcc
      181 gttcaccgcc gacgccatcg aacactccga cgtcactgag gtagccggag aattgcagtt
      241 tctgacaccg aaggacttca ggctgtcgat gaaagagccg gacctacggc gtgcgttgac
      301 tcacctgggc ttcgcttcga tgcggctgaa ggtcacattt ggcgagccca cagcccccgc
      361 acccgtcgcg ccgcaagagc cggcgaccgg tgacgagaat gaactgaccc gccgtgcgct
      421 ggcgcacccg gaagtgcagc ggttccgcga aacgttcggc ggacaggtcc gcacggtgag
      481 aaatctgaaa gaatagtcgg tggcgtgtgg ctcatttcac gccggacgtc cgagtccgtc
      541 ccgaacctcg agccacgggc tgcatgccga ccgaagggag cttccatgaa actgcccgga
      601 aatttgcagg cgatgatgaa gcaggcacaa cagatgcagg agcgcctgca ggaagagatt
      661 gcccaggtcc gcgtcgaggc ttcggccggc ggagggatgg tgaccgttca gatgagcggc
      721 cagaagaacg tcctcggagt caggattgac ccggaagtcg ccggcgacgt agagatgttg
      781 caggacatgg tcatggccgc ctgtaacgag gccgccaaga aggtcgacga ggccatccag
      841 cagaaaacca gcggcctgct tggtggtctt ggtcttcctc ccgggatgtt ttaggagcac
      901 ggagcagcca tgcccgattt tgcggaaccg ctggcacggc tcgttctcga gttcaagcgc
      961 cttcccggca ttggccagaa atctgcccag cgcatcgcct ttcatgtgct gcgcggcagc
     1021 cgggacgacg cagcacggct tgcacaggcc gtactggacg tcaaagacaa actgagtctc
     1081 tgcgccgtgt gcaacaacgt cagtgacggc gagatgtgcc cgttctgcct ggaccccggc
     1141 cgtgacggct cagtcatctg cgtggttgaa gaaccccata acatcatgcc cgttgagacg
     1201 acgcggcagt accaggggct ttatcacgtc cttcacgggg ccatctcgcc actgcgcggc
     1261 gtgggtccgg accagttgcg gatcaagagc ctgctggagc gactcgaagg cagcccggtg
     1321 aaggagatca tcctcgctac gaatcccacc gtcgagggtg aggcgaccgc ctcctacctc
     1381 gcgaggttac tgaaaccgct gggcctcaaa gtgacgcgta ttgcgatggg aatcccggtg
     1441 ggaagcgacc tcgattttgc ggacgaggtg accatgacaa aatcgcttga gaaccgccgg
     1501 gaattgtagg aatcagaaca ggatgtggaa cggatccttc tctcgcaggc cgctgggcgt
     1561 gactccgccg atggccactt cgatcagttg gttcggttcg cgcggcacgg ccacctccac
     1621 cttcaggtag ttatcgctca gcgccgttcc ctcttgttcc agcgtcacaa cggacagccg
     1681 tttccccacc atgcgccgac ggaattcgag gtttttgtcg acggccagtt ctctcagaat
     1741 ccgatttcga tgcttgcgaa ccggaattgg aacctgaccc gtcatctccg cggctggcgt
     1801 gctgggacgc ccggagtatg tgaacacgtg caaatacgta aaaggcaggc tatcgatgaa
     1861 cgaacggttc tcttcgaact cctcatcggt ctcgcctgga aaaccgacca tcacgtccgc
     1921 gccaatcgcc gcgtcgggca taagcgcgcg cgcttttcgg atgcgatctt cgtagtgccg
     1981 gggccggtac ttgcggcgca tccggcggag cacgctatcg gaacccgtct gcagcggtgc
     2041 atgcacgtgc ccggcaatac gcggcgatga cgccatcagt cccagcaggt cgtcggagag
     2101 atccattggc tcaatcgaac tgagccgcag ccgctcgatg gcggtctcct ccagaaggcg
     2161 ccgtacgaga tcggcaagtc tcatctggat gcctgcttcc ctgccccacc ggccgaggtt
     2221 gatgccgctc aggaccactt cgcggtagcg gaggctgagc cgccgtaccc gttcgaccac
     2281 ttcatcgggc tgtgcgctcc ggcttcgccc gcgcacggac gggatgatgc agaaggaaca
     2341 tcggttgctg catccatcct ggatcttgag gtttggccgg gtgcggtcgc cagcagcgtc
     2401 ctcgaccggc gcggcgagga attcctgctg cgcgaaaatg tctccgacga agatctggcc
     2461 atggtatgga gttgtcgcgc cctcctcccg cagaatttcc ggaatccgga ccttgtggga
     2521 gttcccgacc acccatgtca caccgggcat ggcggccaat tcttccggag ctcgctgggc
     2581 gtagcatccc gtaaccagaa ttcgggccgc agggttttcc cggcgcaccc tgcggacggt
     2641 ctgccgaaca tcctcgtccg ccgacgcggt cacggtgcaa gtgttcagaa tgacaagatc
     2701 cgcctgtcgg cactctcggg cggtctcgaa accgtcacgt tcgagcgccg tctccagtgc
     2761 cgccccgtcg gcctgggtag cccggcagcc gaaattttgg atgaagaacg tgcccactta
     2821 cttatgattg tacggaatcc ctcagctgag tgccgatcca cgagtcttac tttccgggcc
     2881 gcaaaccgaa acgccggaca aggatatact ccgccagatt ctcgttggtc aggaggccga
     2941 caagccgatc gccctccatg acgagcgcgc acgccttcga ctgtccgagg agcggaagcg
     3001 cttccacaag atcgatgttg ggtgagacgc ggacgaagtc gcggtccata gcgccggcca
     3061 cgtaggcctc cggcccctcc gacgcgaggg ccttcagcag cgcggtccgg ctgagcatgc
     3121 ccacaacctg ctccccgtga accacgggaa agtcctgctg gctcgtggcc agcagcagat
     3181 tcgcggcatc ccggatggtg tcaccgtggg acagtgtccg gaagtcggtg accatcgccg
     3241 atctcactgg cgcaccttgc atatacaggc gcccgacggc cagtgtacct tcctgggccg
     3301 caccgagata gacaaagaat gcgataaaca ccagaaggaa attcatcgag agcagtccgt
     3361 agaggcccag cgcaatggcg agaaatctcc ccgccgccgc ggcaatgcgg gtggcctcct
     3421 cctcgggcct ccgcagagcc aacagcgagc gcagaatgcg gccgccgtcc atcggaaacg
     3481 ccggcagcag gttgaagatc gcgagaacca gattgccgcc ggcgatcttc acccacaatg
     3541 tcgcggcggc tgggtcttcg agagaggcca gtgatggcaa ccctcccagc tgtgccagtg
     3601 taccgactat cgcgccggcg atgacaaggt tcacagccgg ccccgcaatc gaaatccaga
     3661 gctcttcacg cggtctcggg ttgcgttcaa gacgcgccac gccccctatc gggaacatga
     3721 cgatctccag agtccggatg ccgtagcgcc gcgatacgat cgcgtggccg agttcatgga
     3781 gcagaacaga gccaaacagc gccaggacat atagcacata cgaccagccc gtgctcccgc
     3841 tgcccgctcc aacaaccacc aggaaaacga ggagcagcag gaacgtgaag tgaaaacgca
     3901 ccggcacccc ggcgatccgc acgactccta ccgtcccggg aatacgactg tccgctcggg
     3961 atgcctgccc tttttgcgtc tcggtttcca cgcgcctacc tctcacagta gcagagcggg
     4021 cggatcgttt atgatgctta cttcgcaata caaagtactt gacaatcaga actacttcat
     4081 gaatacttca ctagaggaga atatattcct gtgatcaaac gcatcatcgc catcgcgttc
     4141 atcttcttct gcgcagccgt cgcctgggcg gtactcggag cgaccatttt ctcgcggacg
     4201 tatgattccg acctacggct gaagggccga gtggtctctt cctggggcgc cccacacgtg
     4261 caacgtcctc cccgggcgtg ttacgaccgc cgcgtgccga agactgtgga aacggttgag
     4321 gacggcagaa aggtcctgcg cacgacgcaa gagacggtct caaccgtgct tccgctcgag
     4381 ggaagcaggg tccgtgccgg cctgaatctc gagcaccggc agaagggact gctctggtac
     4441 agcacgtaca gggttgtgtt cggagggacg tacacattcc gcaacacgag cgatgcggaa
     4501 cagcaggtcc gcttcattct ggatttcccc gcggaacagg ccatttatga cgacctgaca
     4561 ttcgccgtag gtggcgtccc ggtcccattc gacggcggag agaaatcggc ctcggccacg
     4621 gcccccgtgc cgcccggcaa gacggtgcag ttgaccgtag ggtaccgctc gcagggggtc
     4681 gagagctggc ggtacaactt cggcgccgac gtagcccaag tgaaggattt cagcctccgg
     4741 atgacgaccg acttcaagga tatcgacttt ccggagaacg ccctgtctcc gtcagagaag
     4801 cgggaaacgc aagacggctg ggacctcgca tggaactaca agaacctggt cacgggtttc
     4861 aacatcggaa tgatcatgcc tgaaaagctt cagccgggtc cgctggccgg gaggatcagt
     4921 tttttcgcac cagtctcctt gttctttttc ttcttcctga tgctggtgat cacaacgttg
     4981 cgcggaatcg acctgcatcc ggtgaactat ttcttcctgg ccggcgcctt cttttcgttc
     5041 cacctgctgc tcgcctatct ggtggaccac gtctcgatac acgccgcttt tgtgatctgt
     5101 tcggcggtgt cgatcttcct ggttgtgagc tatctccgcc tggtagtcgg gatacgattc
     5161 gcggccgtcg aggcgggcct ggcgcagctc atctacctgg tgctgttctc ctacgccttc
     5221 ttcttcaaag gcttcaccgg actggcgatc acgatcggat cgattctgac gctgttcgtc
     5281 gtgatgcaga ttaccggccg catccgctgg gcggaaaaat tctgagagaa gttgagggcc
     5341 ggtttcgcca ccggcccaga ctcaattcgg tgcgccttga agccccgccg ccactggcgc
     5401 gaccatcggc agtgggggag ccagcggttg gaaacggacc gacacgagcc gggagacgcc
     5461 aggctcctgc atcgtcacgc cgtagagcgc ctgcgccacc tccatagttc gcttggcgtg
     5521 ggtgatgatg atgaactggg tctgctcgga catctccttg agcaagcgcg tcagccgctg
     5581 gatattcgct tcgtcaagcg gcgcgtccac ttcatcgagg atgcagaacg ggctcggctg
     5641 atagcggaag atcgccatca ggagcgccag cgccgtgagc gccttttcac cgccggaaag
     5701 caacagcacg ttctggagct tcttgcccgg cggcgacgcg acgatgtcaa tccccgattc
     5761 tccgacgttc gattcgtccg tcagccgcat ctcgccgatg cctccgccaa agagcgtctt
     5821 gaacatctcg cggaagttcg cgttgatcgc ctcaaacgcc tcggcaaagc ggcgccttgt
     5881 ctcgacatcg atgtcgtgga tggccttttc cgtgtcgcga atcgagtcca gcagatcttg
     5941 gcgctgcgta ttaaggaagt cgtaccgctg cctggcctct tcgtactcct cgagggcctg
     6001 cggattcacc ggtccgaggg cttcaatccg cgacttcacc tgttggaact tctcctcggt
     6061 ctccgcaagt gccagttcgt cgatctcagc ttcctcgggt tgagccagtt cctggatcgg
     6121 aacattcaga tctttccggc tcgtctcgtc gaggaacttg agctcggact gacgcttcac
     6181 cagctccagt tcgatctgcg agcgtcgctc ctgcgcctcc tgcgccgcgg cgcgcagact
     6241 cttgagcgat tcttcgagcc cggcgaggga ggcgcgggcg cccgtctcgc tttcagtcag
     6301 ttgcgcaaca cccgactctg cgtctgaaat ctcgcccgac aacgatgccg cgcgctggtc
     6361 caactcaata ttgttagcga gcagccgggc gcgctcaacc ccgagccgct cggtctcgcc
     6421 cgcgatctcc tgcctccgga cggcgatctc gttgagctgg ccctcaatcc gcgtcttcga
     6481 gtcccgctcg gcacgggccc gttcttcctg tcccgccagt tccgcccgaa gggccgagtg
     6541 ttcctcgccg atgccggccg ccgcggtctg aagctgctcg agttcctgtc tggcggcctc
     6601 cagcgaaaat tcctgttgcc ggcgagcctc ctccctctct tccaggaacc ggcccacctc
     6661 ttcccgtttc gcctcggagc gctctttctc ttgccccagc cggcctaatt cgaggtgcgc
     6721 caccgacaac cgggagttcg cccgcgtcag ttcctcagcc agctttcgca tctcgtggtc
     6781 gagcgcaagc gcctcttttt cctggccctg ttgctcggta cggagccgtt cgagttcctg
     6841 ttccaggacc gacacttccc gctccaggct ctcgagcgcg accctgacag cctcgagttc
     6901 cgtctgccgg gagtggaact ggctgctcaa ctcgcgcaac tcacgcttca gtgcgagcgg
     6961 accactgccg gtcttcttgc cgccgctaac cgcctggctc gagtaacaga tgccgtcggg
     7021 aaggaggaaa tagagccctg gatactggga cgccaaccgc tgcgccgcca cccggtcttc
     7081 cgccagaaaa cagcgtgcca gccgtggcag cagttccacc ggagcgtgcg tcaggccgtt
     7141 cgtaagctta aggacgtcgc ttagccgcga gatgattccc gtctcggggc cgatggcggg
     7201 ctcttcgtgc cggagatcgc caccgtccgc gtcgcccttt gggtgaacta gaaacgttgc
     7261 ccggccttcg agatcacttc gcagcaggtc gatgccccgc tccgcctgat tccagctctc
     7321 gactacgacg tattccagct catcgtggag gaactcttcc gccgcctttt cgaatgccgc
     7381 gtcgacatcg acgaagtcgg ccaggacgcc gagcggcttg aggtcgttcg cctggccctg
     7441 gtcgatcgcc gtgaacaggc gcttgaccga ttccgtcgtg tacgctcggt gcgacagaat
     7501 ttcctggagc gagtcccgcc gcgccttgat gccggagaat tcggaccgca gccggtcgag
     7561 ttgctgccgc gtctcggcgg ctcgggtccg gcggtccgcc aacgcttcct ccgcacggcg
     7621 gcgctgctcg acggtcgatt cgagttcgag ttgccgggca gccgccttct ccgataattc
     7681 tttccggctg gtctccagcc actcgagatc cgtgagcgcc gattgctcct ctttccgggc
     7741 tcgcgccgtg tcgcgctcca gcgatgccag gtacccttcc acctgcgcaa gctcgttctt
     7801 cagcgtcgac gattcgccta gcagccgcaa tacggactgg cgcgtggcct cgatctgctg
     7861 ctcgcggccg cgcaatgaat cctgaagctt ctcgcgctcc gtcgacttcg ctgacagccg
     7921 ttctcgcgat gaatccaact gctgctccag ctcggcgatt ctggcgagtt gggcatccag
     7981 ttccaattgc agcgcagtgg cgcggcccga aagctgctcg gcttcggcct caccctgcga
     8041 aagccgctcc tcgatggaag caatctgctg tgcttggttt gccaactggc cccgtgtgcg
     8101 ttccgcttcg agttggagat ccgcaaggcg gcgacgcgcg ccggtaagtt cgttctcgat
     8161 ccggtacgtt tccttctgga gccggctgta ctcctcctgt cggctcgcga cgtcgcccga
     8221 aatccgttgg aactccgtca ccgccacgtt cagatcgagg gcggtcttcg cggcgtctcg
     8281 ctccagcatg cgatatcggc ccgacaacag gcggcgcaac tgggcgtcca tctccgtctt
     8341 cagttcctcg tagcgtctcg ctttcgaggc ctggcgcttc agtgaattca cctggcggcc
     8401 gacttcttcg agaatgtcga acacccgcgc caggttctgc cgggcgcctt ccagcttcgc
     8461 ctccgcgagc cggcgtttgg tcttgaactt cgtaatgccg gccgcctcct caaccaccga
     8521 gcggcggtcc tgcggacgcg agctcaggat ctgccctatg cgtccctgtt cgaggatcgc
     8581 gtagctttcg ggcccgagcc cggtgcccat gaagatgtct tgaatatccc tcaaccgggc
     8641 cagccgccca tcgatcaagt agtcgctctc gccggagcgg tagaggcggc gcgtgatcgt
     8701 gatttctgcg gggcgctcct tcgctgcgcc attcgcgcca ttggcctccg cgccatgcgc
     8761 ggcggcttcg acggcgcctg tcccgttcgt acccgagccg ttcgtggctt ttccgttcga
     8821 ttttgcgtgc gcagcggagt gctccacatg gactgacgga tccaccagag tcatcgtgac
     8881 cgacgccatc ccgagcggct tccggtccct tgttccagca aagatcacgt cctccatccg
     8941 ggtgccgcgc aaagatttcg cggactgctc gccgagcacc caactgatgg cgtcactcag
     9001 gttggacttc ccgcagccat tcggcccgac gatcgcggcg atcccgctgc cgttgaagcg
     9061 cagttcggta cgttcacaga aggacttaaa cccctgcagc tccaaccgtt taagttttag
     9121 caaggcgcgt ttcctttcaa ttcgccatgt gcttccgcac gcctcggcgg cggctcgggg
     9181 gatacctaca tcctaaaacg ggtccgggca aatgtaaagg acaaagacca cagcatggcg
     9241 cgggacttaa gcctccgccc caagatatag tactgcgctt ccctagcgta tgtatttctc
     9301 gacccagtcc aggtggcgtc gcatgatgtc cagcataaac ttcggctcct gaggaccgtg
     9361 cggttccctt gggtagacca ccatcttcgc cggaatccct tgtcttttta gtgcgttata
     9421 gaactcgtat ccctgagaga tgggcacgcg tatatcccct tccccgtgca ggatcaacgc
     9481 cggcgtcgtt attcctttga cgtacgacat cggggaatgt ttcctgtagg cctcgaagct
     9541 gtcccagggc tctcctgcga agaaatcggg caggaagccg gggatgtcgg atgtgccagt
     9601 gaaactccac agattcgtca ccggcgcgcc gatcgctgcc gccttgaatc gccgcgtgtg
     9661 cccgatcacc caactcgtca tgaatccgcc ataactccag cccatcactg cgagtcgccc
     9721 ggggtctgcc acgcccatcc cgatgacgtg atcgatcccg gccatgaggt cgtcgtagtc
     9781 tttaccgccc cagtcattgt agttggcgaa gcggaactgt ttcccgtagc cgccggaacc
     9841 acggatgtta caacgcagca cggcgtaccc cttcgaggca aacgccgcca gcggatacag
     9901 tccgggccgt cctggataac tctcgttaaa cacccccatt gggccgccgt ggatgttcag
     9961 aatcaacgga tactttttcc cggactcgaa ccccaccgga taggtgagca ggccctcgat
    10021 gtcgaggcca tccttcgcct tccagcggac cagcttggtc tctccgatcg acggtagcac
    10081 cttgtccgtg ttcgcccggc tcactcgaac cggcgaggtc gcggacagcg acaaaacgca
    10141 cgcctctggt ggttcgaggg aactttcaca tgtgaacccg gcgtgcgtcc ccttggcatt
    10201 gaggcgcacg ctcggcccgg cgacgccttg ctgcggctca tacacgagtt tcggcacgcc
    10261 atccaccggc attgcgtaca gcgcggtccg cgtttgcttg ttctccgcaa agtaaagcac
    10321 cgacgaatcg ccggaccacc ccgcgaacac gggccgttcg tcgtagcttg ccggcaaaac
    10381 ccgaagctga ccgccctgtc tcggaagcag ggcgacacgg gcatcccctc cccaccggac
    10441 cggctcggtc gacaccgtgt aggcgaggta tcggccgtcc ggcgagtata gcggcgtacg
    10501 ctctgctgcc ccggtcaccg caagcggccg gacggtcccg ctttccacgt cgacttcgga
    10561 gatgtccgtc agcctccact cgtccgcatt tggcgtcggc tgatgctcga aggcgatctt
    10621 gcgggaatcc ggagcccagt caaggtctgt gatgtgatac gcgttcgcga acagcttccg
    10681 cgggctcgtt gttccactcg caccgggctc ggcagcgatc acccacaggc cgtggttctt
    10741 ggggttttca tcgactacgc ggaagtcgcg cttctccttc ttagacttct cctcttctgg
    10801 gtcgtccggg acgccggcaa acgcgatcca ctttccgtcg ggcgacactc tgtacgcgcc
    10861 gatcgatccc ttccaatccg tcagtatctc ggcttcgccg ccggcgactg aaatccgcca
    10921 caggttctgt ttgcccgaac gagccgattg aaagtagaca tacgaaccgt ccggggaaaa
    10981 cgacggcgca ctcgagcttt tctctccgcg cgtcaactgg acccgccagg agtcgtcgct
    11041 gcggcctaga tagatctgcg agatcatctc gctcttctcg gcttccatca cagctcgggt
    11101 ttgggtccac gcgacaaggc ggccgtccgg cgaggggacg gcgctgccaa cggtctgtat
    11161 ctccatcgca agctctggag tccagccgga cggcgtttca gccgcttcca tattggggag
    11221 cagaaacaga agcgcgagga tcgtcttctc cataggcaca tggtaacgcc ggaacacgca
    11281 gttatccgtg agtcgtcggc gccgcgcggt tacaatggga gactctggag cgcgcatgag
    11341 tcaaagcatt gtcctggcgg atgtgagccg ctcgctggcc cgcttcctgc ggaaagagtt
    11401 acaggcggac cccgcggtgg gggagtggat ggacagcgac gagcgcatct cgctggactc
    11461 gccccatgcg ttgcaagagg acggcgccgt ccgtctttcg ctttacctgt atcatgtctt
    11521 cacggatcag acgccccgga cgcgggtccc gatgcgggtc gaaccgggtc cgcgccacga
    11581 accgcctctc gttgtgaatc tgctctatct cgtcacgccc gtgaccggca cacctgtaga
    11641 acaacagacg ctgcttgccg cggccatgcg ggcgcttcac cgcaagcctc ttgtggaggg
    11701 gaccgacctc ttcggcgcgg cggcatcgct cggcgagccg gtccggctga cggcgagccc
    11761 gctcacgctc gaggaaactg cccgcctgtg gcaatctctc ggcatgagcc accgtcccag
    11821 cgtctgctac gccgtacgtc ttgttctgcc ccagtcagag tcgtagcggc gcaccgcaat
    11881 tagccgtcta ctcctctggc acaagagtca tccgttcgat ggtcgcggct tcgaccgcct
    11941 tgcgcgagaa cagcatcgga tggtagcggc ccgccagcca cccgtcgagc tggtcactgt
    12001 atcgcgggtt ttcgggattg cccgactcac ccggtacgtt cgtcatcacc gaacggtccc
    12061 agtctccgag atcgataatc tgccggtagg aggcgccgtt tgcttgacgg aagtcggtcc
    12121 cgctggcggc atttacagtc tgtgcgtctc ccggtcgctc gaatgggccg cagtggaaag
    12181 ccgcgcggtt gaggtcgtgc ctgaagtgga gctggtgtag tctgccccat cgccactgtt
    12241 tgcgatcggg tccgagccgg ctttgtagtt ctttcagggc ggcccccagc gagcgctgga
    12301 gtgcattcgg ttcggaccgg gcctccagcg ttttgagaag catcgctaaa tccgtcctcg
    12361 caccgagcgc cggaccgaac agctccgccg gcagtttcga gatccacacg gcgtagatcg
    12421 cagcgggaac ggagtctacc gccatcgcgg cgtcccatcg caacaactgc tctctgatct
    12481 cgccggcctc gctgccggtg ggcggattcc acttctgcaa caccttctgg aagcgacggg
    12541 ccggaactga cacgatatcc tgctggaagc gctggaaatc ggcgacgctg aatttgttcc
    12601 ctccggagat cagttcaagt atcctctcgt atcgataagg cagcgcccac tcgtagccaa
    12661 tcggaatccg gtaatccgag ggcagaatgt tgtggttggc cgtcgcgatg aagtgggacg
    12721 gcgggttgaa cttgcgcggc agttcggcta ctcgccggaa tccgctccat tcatactcac
    12781 cagtgtgacc cgggactgga aacagcccgg accagttcgt ccggatcggc gtcagccccg
    12841 ccgcctgcca gccgatgttg cccgctgcat ccgcatacac gatgttttcc gacggcactt
    12901 tccaacgttc cattgcatcc agaaactcat tccaggtact cgcacgggcc accgccaacc
    12961 ccgcgaaata gccggccgtt cccggctcgc tgccgaccca tcgaagcgcg tacgcgcgcc
    13021 gccgctgtgg gtcctcctgg atgatcggac cgtgaatcgt gtgcttcagt tcgacggcgg
    13081 cggactccgt ttcgcctctc actggaattc tgaccctctc cacctctatc gcccgccact
    13141 ctccgcggta aaggtaccgg ttcggatccg aggggtggag cctctctacg taaagatcct
    13201 gttgatcgat gccgacaatc gtaaagcccc acgcgatctt tccgttgtgg cccagggcaa
    13261 tccccggcag cgccggctcg cctgcgccaa tcaagctcca gccgggggcg acgaggtgca
    13321 cagttcgccg cagggaagga atctgcagcg gccggtgtgg gtcgttcgcc aggattggct
    13381 tgccggtggc cgtcatcgtg ccgtcgacaa cccagttatt gctgccctgc tcctgcgaaa
    13441 acttcactgg cccgacagcc tcgttgtaga cccggagcac ctccgcggtc acatcgtcca
    13501 ggtcgagccc gttcggtatt cgcaagggaa tgaacggatc gggcgggagg aagtgctgga
    13561 gtgtcttgag accgaattgt cgtgcgtcct cgacgcggcg cacctcgcgc tggacgttcc
    13621 tagtcatcag gattccggcc atgcgagcga gacagtcctc cggctcccag agcccaggat
    13681 catagccggc gattcgaaac tcgagcagcc gtttgccgcc cagttcgcgg atgtatgcat
    13741 tgatgccgct cgtaaatgac accgcgatgt cccgggcgcc ggggccgtag ctctcccatt
    13801 cggcgctcca gttcccgcgg tatcggacgg cccacgccag tccgtcccgc ggaagcgcgg
    13861 tggggcccag tgcctccgcc agcttgcctg ttcccacccg ccgccacagg tcgatctgcc
    13921 agaggcggtc ccgggcggcg aggtagccct gcgtgaaaaa caggtcctcg gtgttacgag
    13981 cgtagatgtg cggtacgccc cagcggtcgc gaagaacctc gacgggctcc ttcagaccac
    14041 gaatttggat gtcggcggcg ccggcgaagg gggcgagaag caggagaatg aggagccctc
    14101 gcaacatggc ggcattgtac acgagtgcga ggtcgcgcgg tacgatatcg ggcatgcccg
    14161 gcggtaaatc aaagtagacc aagcgcccat gagacagctt ctgccacttc tgttcgcgcc
    14221 cttcgtgctt caggcccaca tggtaagtct cagcacgggc gagattcgca tcgaggggca
    14281 acgcggatat tacgagttgc gcatgcctct ctacgagatc gcccacgtgc gcgacccgga
    14341 gcggaccctt tttgaacaca tccgtttcgc ctcgggaggc tctcccgctc gtcaggtcag
    14401 cagatcctgc cgggaggacg ctgaagcggg cgcatacatc tgccgtgcgg aatacgtttt
    14461 ccccgagccc gcggtcatcc tcgaaatcga gtgcacactc cacgcggtca cagtgcccaa
    14521 ccacgtccac ctgctgcgtg cggtcaacgg cgggcggacc gaccaggccg tgctcgattt
    14581 ctccttttcg aaaaccactc ttcgtttccg gccgcccact acattcgaga ccgccacgac
    14641 tcacctaagc gctggcatga tgcgggctgc ggggggcgcc gctcagctat tgttcttagc
    14701 cagcctggtg ctcgccgcgc gccggcggcg cgagttgctt gcgctcaccg gaatgttcct
    14761 ggcaggagag gtcgcctcgt gtttgttgct accgatcacc ggctggcagc ccgcgccaaa
    14821 cttcatcgag gctgcggcag cactcaccat cgcctacctc gcagtcgaga tcctgctgct
    14881 gcccgaggcg ggcatgcggt ggctcgtggt cggcgtcctc ggagctttcc acggtttgta
    14941 cttcgagata ttcatccgta acagcggcta ctcggctctc tgggtgctta ccggtgttgt
    15001 tctggcggaa gtactcctga tcgcggtgtt cgcgctgatt ttctcccgca tcgccaaagc
    15061 caccgaggcg cttcacccgg taaaggtctc ggcttccgtt ctccttgtgg tcggcgtggc
    15121 ttggttcgtg ctgcgcctgc ggggctgacc tacctcggcg acaacttcgg ccactcgacc
    15181 tcgatcccct gctccacgat tcgtgtctgg aactccttga gcagcttctc cgagttacgt
    15241 atccggcgcg gcgggttctt cgtgtggatc gcatgcgcag ccaccgcgcc cgccgactcg
    15301 ccgatgttcc actccaccgg atgcagccgg taacagccgt tggtgatatg cgtcaccccg
    15361 aggttcttcg atgccggcag caggttctcg acgcgcttcg gaatcagtgc gcccagcgga
    15421 atctggaatg gcagcgaact gacgtcaatg tagttgtcac cgcccgagct cgggtgcagg
    15481 tcgatccggt agctgcccac gcccacggaa tcgccgaaga cctcagccgt cagttcttct
    15541 cgtttgcggc cggtcagctt cattcgcgca tcggtgccta cgtgctgctc gagaaccgtg
    15601 aattcggcct ggatccgtcg cgactcgcgg atgtacggat atttcgccag cccgtcctcc
    15661 gtgccggtga tgtcgtggcg caggcgcaag ccgcgaaagc cgtggccgcc gtccgggcgg
    15721 ggagcctccg tctgcatcca gtagaggagc gaaaggctca actgctttcc ccggagcagg
    15781 tgccctgccg cctcatcttc actgacctcg tgcaggttgc cgagccagta gtcgttctgt
    15841 ggccaattga tcagcgagat gtcgcttcga taggcgccct cggcgaagtt ccgccggttg
    15901 gcgatccgcc ggtacagcca caggttcagc cgttgttccg ccggtgcttt cagctcggga
    15961 tcgaacatca ctggccggag ttccagcgtg accggattgc tcatcgacca gctcagaagt
    16021 ttgcccggcc atggcggtct caactggggc acgtagtcac gccagaagga gtattcgtcc
    16081 ggcttgtcga tcgtatggtc ctcgccctcg atgtggtcga cagcgaagca caccgtgaac
    16141 gcctgcatgt tcgccggctg cggctgttcc ggagcgtgcg gttcacccgt ctgcttgcga
    16201 gactcgaagc cggtcacgta ctcggttcta gtcagcggca gcaggtcgcc ctgttcggtt
    16261 gcatccacga agtacgccgc gtaaacggtg cgttcccggc cgctctccac gcccttcacg
    16321 gtgacggacc gaaccgagtc cttatccacg tccgccgaaa cgggcttgtg acgaagcagg
    16381 acgacaagtt gcccgcctcc gaggtacggc gccagcatcg actccagcac ggcgagggac
    16441 acacgcggct cgtgcgtcag ccgggagacc ccgccgtcgc cggggttcag gtatctacgg
    16501 ccccgagcga cggccgtcag tgggtagtga tcccggtagt attgccggac tgcgtcacga
    16561 tacgtgcgga acagccgggt gcagccgaac gactcgatcc actggtgttc gtccggcgga
    16621 accgcctgcg ccgtaagctg gccgcccacc cagtcggtct cttccgtgag gatcacccgc
    16681 attccgtccc gtagcgccgc cagcgcagcg gcacaaccgc cggtaccacc accgatcacc
    16741 gctacctcgc aacggaactc ccgggcgccg gccagcaccc ccggcagcaa cagcagccca
    16801 cccgactgtc gtaggaagtc tctccggttg gcttgcatca caccggaatc atagaacaac
    16861 cgacgccgct ccgatgattc ccacgttttc ggccagttcc gccggcataa tcggagtccc
    16921 ggccgcgaac tggttgatgg tgcgcgacgg cgcgatgcgc cgaagcctcg agaacagggg
    16981 ctctccgatc cgggacaccc cgccgcccac cacgatgatg tcggggtcga gcaggctgat
    17041 catgctcccc atccaggcgc cgagcatcga cgcgatttca tcgagaattc gcagcgactc
    17101 cccgtcaccg cgggcaaccc catcggcgat ctgctccgga gtccggccgc gcttcgcgat
    17161 cgcgctgccc gaagccagcg cttcgatgca ccccggcgtt ccacagttgc agatcgtgcc
    17221 gcttcgccag tcgatggtaa cgtggccacc ctcggcggcg gcgccgttct tgccatggta
    17281 gaccttcccg tcgagaatga tgcccgcgcc gatgcccgtg ctcagtgtca cgtacaacac
    17341 gctgtcgaat ccccgagccg ctccgaagcg cgcctcggcc agacccgcgg cgttcgcgtc
    17401 gttctccact cggcaaagca acccgaaccg ctcgccaacg agattcgcca gtggaacatt
    17461 gcgccaacca ggcaggttcg gcggattcag gatcacacca gtctttgggt tcagcggacc
    17521 cggcgcgcag atgccgatcg cccgcacgcc ggccggcagg cactcgccga tcgccttgta
    17581 cacttgcccg atcgagacgt cgtagccctc ggcggcacgc gtcggaaccg taacaccatg
    17641 gaccacgcag gccccatcgt tgacagtgcc ggcggcgatc ttcgtgcccc cgatgtctac
    17701 tccaaggacc gacatgctgg tacgatatca tgtgcttggg acgctaatgg cactcacttt
    17761 gcgaggccgc gaagccgggc aatgggtctg attacgattt ccgggcagcc gggttgccgg
    17821 cacgatgaag tagcgcgttt aaccgcgcag cgcctcggat ttgagctggt caccgaaacg
    17881 cgtctccaca gcttgattga gcgggagttc ggccctgcca caccgatttc cgacaaggcc
    17941 taccggcacg tacttctctc gatcctggca cgcctcgcta cggagcacca cctcgttgtg
    18001 agtgcgttcg gcgttgagca tgtcttccag aacttccttg gtgttcttcg cgtgtacgtc
    18061 gtggccccgg agttccggcg gatcggcacg ctcatgctgg accgccggct ggaccgccct
    18121 tccgccaagc gttcgctgcg tcaaatggag cgggaggaga gcgaggaacg gcggcgcaag
    18181 ttcggacggg ccaccgttcc gtctcacgca tttgacgttg tgttgaactc ggagttcctt
    18241 gaaagcgacc agcttactga cctgatcgag gcggctgccc gcgcgcgatc cctggtcgaa
    18301 caggggctcc tcagttccgc cgccgatgcg cagctccaat tccaagccag gctccagctc
    18361 tcccgctacg gcatcatgcc ggcggacgct gtcagcctga aacgcagcaa cttctcccac
    18421 ccgagcgagg agatcttcgc caaccttctg gacttctacc gtgtcgcatg ggaatacgag
    18481 cctcgaagct tccctgtcaa gtgggacaag gacggtcgcg tccttgaggc gttcacgccc
    18541 gacttttatt tgcccgagtt cgacctctac gtggaactga ccacgatgaa gcagcccctg
    18601 gtgacgaaga agaaccgcaa ggtaaagctg ttgcgtgaaa tctaccctga aatcaacatt
    18661 caggttttct accagaaaga cttccagaac ctgatcttca agtatggcct cacggagcgg
    18721 ctcgcccgcg tatgagtaaa gcccagcccc ccggtattga gcgaatcctt ttcacggagg
    18781 aacaaatcca gcagcgaatc cgcgaggtgg cgggcgagat ctccgagcgt ttccgcggac
    18841 gcgacctgaa gctgatcggg gtgctaaagg gctcgatctt ctttctgacg gcccttgctc
    18901 gcgagattga gattccgatc aagatcgatt ttcttgggat ttccagcttc tcgaaccgca
    18961 ccggggctcc gggcgtggtg cgcatcgcca aagacctcga cgagagcatc gagggcgaag
    19021 atgttctcct agtcgaggac attgtcgaca ccggcttcac cgctcgctat ctcctccaga
    19081 cgctcgctgg ccgggggccg aattcactcg ggatctgtac gctgcttgac cgcacgtcca
    19141 gccggatcgt ccaggtatcg gtggaattcc gctgctttga aattccggac cgttttgtgg
    19201 ttggttttgg cctcgattac aagcaattct accgcaacct tggcttcgta gccgttatga
    19261 aacgcgatta actgcaactc taactccttc ataactctgc cgccctgaat ccgtcagtga
    19321 taaccctctt ccaccggcga agtttatgtc ttttgtttgc aaacacgatc gacaagagtc
    19381 ttgaagctcc cgcagatcgg ccttattatt agaagtgcct gcgtcaggag acggtaccca
    19441 tgcagcaggg agtgctgttc atttcttctt ccgctgagga cgccaacgcc ctcacgggaa
    19501 tgctgggtcc gctgtcgata cctctcaaac acgcgacgga tctccgtttc gcccgtacgg
    19561 cgctggatag cgagaaatac ggagtcatcc tgacggaagc ccggctggct gacggcaact
    19621 gggaagacgt catcaacatc gtggagaagc aagacccgtc ctgtgcggtc gtagtaaccc
    19681 accccttagc cgacgccaga ttttgggccg acgccattga aaccggagca tacgacgttc
    19741 tggctcaacc gttctgctgt tccgaggtgc agaggatcct ctcaaacgcg ctgtcatcac
    19801 cggtcgaatt gaagagactg agccgcgcgg ccatataatg gttcttccgc ttaggaggaa
    19861 ccgatgtcgc aacccgcgca ccagtttatc gagcaaaatc gagagcgatt cctggaggag
    19921 ctgaaagagt tcatccggat cccgagtatc agcacgcttc cagagaacca gggagacatc
    19981 cagcgtgccg cgcggttcgt gggcgaaagg cttacagggg cggggatgga gaacgtcgag
    20041 gtcatcccga ccggcagcca cccgctggtg tacgccgact ggcttcatgc gccggggaag
    20101 cccacggtct tgtgttacgg gcactacgat gttcagcccc cggacccgct tgaagaatgg
    20161 gtatccccac ccttcgagcc aaccctgcgt gacgggaacc tctatgcgcg cggcgcggtg
    20221 gacgacaagg gacaacttta cgcccacatc aaggcggttg aggccttgcg ggcggtaaat
    20281 ggatcccttc cggtgaatct gaagttcctc atcgaaggcg aagaggaagt cggtggcgcc
    20341 tccatctcga agtacgttga acagaacccg gagaagctga aggccgacgt ggccctggtg
    20401 tccgacaccg cactctatgc agaaggcatg cccacgctct gcatcggcct ccgaggactg
    20461 gtgtatatgg agatcgaagc gaccggcccg tcccgcgacc ttcactccgg actctatggt
    20521 ggcgcggcgc cgaatccgat tttcgggctc gtcgaattgc tgtcgaaggc caaaaaccgc
    20581 cggggtgtca tacagattcc ggggatctac gacgatgtgg aaccgcctgc gccggcggaa
    20641 ctcgagagtt ggaccagtct cccgttcaat gagaaggagt tcctgaagaa agaggttggg
    20701 gcctcgaagc tcactgggga acccggccgc tccgtgctcg aacgagtttg ggccaggccg
    20761 accttagaag tacacggcat cgccgggggc tttaccggag cgggcgcgaa aaccgtgatc
    20821 ccagcgaagg caaccgccaa agtcagcctg cgtctcgtgc cacggcaaga ccccgagaag
    20881 gtcatcgccg ctgtccgtga gttcgtccga aagaagacgc cgaagggtat ccggacggag
    20941 gtccgggtct tgagcgccgc gcccggagtg ctggtcaacc cggatcatcc tgccgtccac
    21001 gtcgccgcga aagtctttgg agaggtattc ggacgcccca cggtcttcat tcgctccggc
    21061 ggctcgattc ccatcgtcgg cgaattcgcg aaacacctcg gcattccgac agtcctcatg
    21121 ggcttcggac tgcctgacga cggactccac tctcccaatg agaagtttcg gcttgagaac
    21181 ttctatctgg gtatcaagac cgtctcgagc ttcctggaag agtacgggca gaaccggtag
    21241 cgtcttggcg cagcagcacg agaaaatcgt ccgatccgcg ggggtagtgt ccgcttctgt
    21301 tttcgccagc cgcgtgagcg gtctggtgcg cgagatcgtg atggcccggc ttttcggcgc
    21361 cggccagctc tatgatgcgt ttctgcttgg gtttcgcatt cccaacctga cccgcgatct
    21421 cttcgcggaa ggagcactct cttcggcctt tgtcccgact ttcacccagt acctgaatac
    21481 aaaaagccgc cgggaggctg ccgtccttgc gaatctggtc accactgcgg tcctaatcgt
    21541 cgtcggcgct ctgtgtacgt tgggcatgat cttcagtccg gaactcgtcc ggcttctcgc
    21601 ccccgggttc gccgccgtgc cgggcaagtt cgagctggcg gtacaactca ctcgcattat
    21661 gtttccgttc ctgctgctgg ttgcgctggc ggcgcaggcg atgggaatgc tgaacgcctg
    21721 tggacaattc ggcgtgccgg cgctgtcctc gacgtttttc aacataggtt ccgttctgtt
    21781 cggactggta gcaggcttct ggctcggtcc ccgcctcggc tttgagccca tcacaggcat
    21841 ggccatcgga gtggttcttg gcggcgggct gcagttgctc tggcaagttc ccagcctgag
    21901 acggtcgggc ttcaccgtca ggctgcttac ggactggtcg catccgggcc tccagcagat
    21961 cctccgacta atggggccgg cgatcctcgg caatgcagcc gtgcagatca acgtgatggt
    22021 caacacgaac ttcgcctcag gcatccacga tcccgtgacc ggagccgacg ggcccgtgag
    22081 ctggctgggc tacgccttcc ggttcatgca actcccgctc ggcctgttcg gagtggccat
    22141 agcctccgca acgctgccgg ccatctcgca gagcgcgtct ctaggccgct ttgacgaatt
    22201 ccggcagacg ctctcacgct ctctgggcat ggtctttctt ttgacactgc cctcctcggt
    22261 cggactggca gtgctgggcg agtcgatgat tggcgcgatc tacgaagggg gaaaatttcg
    22321 ccattacgat acgcagcaaa ctgcactcgc gctttcgttt tacgccgtcg gactcggcgg
    22381 ctacgcggcg ctcaaggttc tcacgccggc tttctacgcc ctgcacgacg cgcgcacgcc
    22441 gatgttcatc agcctggcgt caattctgat taactacatc gttgcggctg cgctcgtgcg
    22501 ctcgggcacc ctcggacacg ccggcctcgc catggccacg tcggcggtag cctggttcgg
    22561 attcgtcgcg ttgttctcga tcatccgcgg gcgtatcgga ggagtatacg gacgccggct
    22621 gttcgagagc gtgctgagga tctccgccgc gtccatcgcg atgggtgtgg cggtagtgct
    22681 ctccagccgg ctgctcgaat cttggcttgg tgggtcgcgc ctcgggcggc tcgccgacct
    22741 cgccatctcg atcccaatcg gagtgttcgt cttttatggc gcctgccgcg ttcttcgagt
    22801 cgccgaactc gagctcgcgg gccgtgcgct cgccgcaccg ctgtcagctt ggctgtcgcg
    22861 gcagcgtggt aaaatttgat tctcgatggg cccagacatc aaactggtaa tcgccttaca
    22921 aaatttagat gcgcgaatcg gggagttgca gttggagatt ggcgcgctgc cgaaacatat
    22981 ttcagccatc gaaaggacgt tggaatccca tatccgcaaa ctggaggccg accaggccgc
    23041 cctcgccgcc aaccagcggg aacgcaagaa actcgacgac gacatcaagg ttcagaacca
    23101 gaagacctcg aagttgaagg accagatgat ggaggccaaa accaacgagc agtaccgggc
    23161 cttccagcac gagatcgatt acggcatgaa ggaaatccgc aaggccgaag accgcattct
    23221 cgacctgatg gcggagtctg agccgctaga acagaacctc cggacggccg aattggcgct
    23281 caaggatgag aaggtcaggg tggagtcgga gaagaaagcc gcccgcgagc gcacggcaaa
    23341 ggacaagcag gaactcgacg ttctaagtac cgagcggcgg caactggtgg cggaactcac
    23401 ccctcaagtg tattcggctt atgatcgcat ccgtaagaaa aggcggggcg tcgccgtggc
    23461 ggaggccgtc gatgggcgtt gtggagcttg tcacatggct ttgcggccgc agttcttcca
    23521 ggaccttagg gcgagcgagc aggttatgtt ttgcgagagt tgcgggcgga ttctctacta
    23581 caatcccgtg atcgtcgtcg agcctgacat gtccgcggaa gcaggcaccg agccgccaca
    23641 atagcgccat acagggtgcg gaaccgaggc ttacccagtc gaacaactgc ccgcttggat
    23701 atgttaccct ctacatttta gtgcggttcc tcacgccaga accggcctca acaagggagt
    23761 agatccagtg aaagtttatg aaggaaaaga catccgcaac gtcggtctga taggacacgg
    23821 ccactcgggc aagacgaccc tggctgccgg cgcgctgttc ctcaccggtg cgaccaatcg
    23881 cctgacgagc gtggacgagg gcaacacgct cacggacttt gacgacgagg agatccagcg
    23941 caagatcagc atttcgaccg caatcgcctg tctggaatgg aagaagcaaa agattaatgt
    24001 cctggatacg ccgggcttca atacttttat aaatgacact caggccgcac ttacggcagc
    24061 cgacgcggcc ctcgtcctcg tggatggggt cgcgggtgtc gaagtgacca cggagaaagt
    24121 ctggagtttt gccaagacct acggacagcc gagggctttg gtcatcaaca agctcgatcg
    24181 ggagaactcc agctttgcca gagtgctgga gagcatccac gagaacttcg gccgcacggc
    24241 ggttccgatt cagttgccga tcggctcgga acgcgaattt cggggtgcaa tcgacctcgt
    24301 tcggatgaga gccttcatgt acggccccga gccctccggc aaaggcaacg aggaggacat
    24361 ccccgccacg atgaaagaag cggcggagaa ggctcgtgaa actcttgtcg agatggtggc
    24421 ggagggcaac gacgccctca tggaggagtt cttcgaaaaa ggtacgctgg cccccgagca
    24481 gatccaggaa ggactgcggg cggcagtgaa ggacatgcgg ctgttcccgg tgctctgcgc
    24541 ttctacgtct aaaaacctgg gcacggacct catcctcgac tttgtcgtgg actacttccc
    24601 ggctccgacc gagcgcggtc cgctcgccgg cgtcgttgac ggtgcagaga cgactcgttc
    24661 g
//
LOCUS       DATIFQ010000518         6517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2860987, whole genome shotgun sequence.
ACCESSION   DATIFQ010000518 DATIFQ010000000
VERSION     DATIFQ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2860987"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VLE22_26120"
     CDS             complement(<1..1146)
                     /locus_tag="VLE22_26120"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HSB17954.1"
                     /translation="MRLDRRKFLAGAGAVALGSGCGRAPEKGARRTEGPSPEDLDRAL
                     TTPVLKRDGLTSPVVIDSIRLLKKDREYFVHVRSKDGAEGVSLTNPPRAEHLHPILNQ
                     LVIPFFIGKDARDLENLLWELYRWQSNYKLYGLAFWSPQAWVEFAILDMLGRIARKPM
                     GALFGDIVRREIAFYVASGRRDTTPEQEIENLRGLLEKSKAKALKFRIGGRMSRNADA
                     MPGRTETLIPLVRKTFGDAIDIHADSNSSYDPPKAIEVGRMLEEIKAVYYEEPCPFDH
                     LEDTKKVADALTIPVAGGEQEYSEWRFRWMIANRGVDIVQPDLHYYGGMIRSIRVAHM
                     ADVANMPTTVHISGGFGFVYMLHFASCVRDPGRYQEYKMGTEKYGAWF"
     gene            complement(1287..2306)
                     /gene="fba"
                     /locus_tag="VLE22_26125"
     CDS             complement(1287..2306)
                     /gene="fba"
                     /locus_tag="VLE22_26125"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01521.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HSB17955.1"
                     /translation="MPLVSMRQLLDEAAKGGYGVGAFNVNNMEQIQAIMEAARETKSP
                     VIIQASRGARSYSQDRFLYHLMLAAVELYPEIPVVLHLDHGNSVATCKSAIDMGFTSV
                     MMDGSLMEDGKTPSSFEHNVKVTREVVETAHAKGVTVEGEIGVLGGIEDGHGAGGSGL
                     EHITDPDQAVEFVKQTGLDALAIAIGTSHGAYKFTRKPDGAVLKMDRLIEIHKRLPTT
                     HLVMHGSSSVPKDLQDIINQYGGKLRPTWGVPVEEIQLGIKNGVRKINVDTDNRLAIT
                     GAIRKVFIETPEKFDPRDYLKPAREAMKKVVAQRMQEFGQAGHAGDYAPLPLAEMAKR
                     YKNGN"
     gene            complement(2463..4001)
                     /locus_tag="VLE22_26130"
     CDS             complement(2463..4001)
                     /locus_tag="VLE22_26130"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSB17956.1"
                     /translation="MSDKVAIFDTTLRDGEQAAGTRLGSREKLTIARQLARLNVDIIE
                     AGYPASSPEDFVAVQLISTEIEGPAICALSRAVASDIEACGKALAKAKRPRIHTGIGV
                     SDIHIMGKFRDEKYGTTLDEKRATLVQMAIDAVKLARSYTDDVEFYAEDAGRAEPAYL
                     CEMLQAAIGAGATVVNIPDTTGYTVPEQFGALIRSITENVPNISRSTISVHCHDDLGL
                     AVANSLAGVANGARQVEGTINGVGERAGNAALEEVVMAIRTRGDYFRVVTGINTTEFC
                     RTSRLVADMLGMAVPPNKAVVGGNAFSHSSGIHVDGFLKQRETYEIMRPEDVGFSDSR
                     VVLTARTGRHGLRDRLEKLGYALSQGELDLTYQRFLRLADKKQEVFDEDLVAILHDEI
                     HPVPETYQLEYLHIYSGTSAIPTATVRLRVRGDLREGAAVGDGPVDAACKAISQVAGS
                     SANLVRYEIRAVTSGTEAMGEVTVNLEAAGRRVMGRGASTDVIEASARAFVDGLNKLA
                     GFAG"
     gene            complement(4055..5134)
                     /locus_tag="VLE22_26135"
     CDS             complement(4055..5134)
                     /locus_tag="VLE22_26135"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HSB17957.1"
                     /translation="MRRRDLIGMAGLAAWPGQAESAEVRTEVVRLKLRHTWTTVMSSS
                     DYRDNLHLRFKRDGVTGFGEAAPIVRYKENADSARKAVQSVADLLRSADPWYFSKLLA
                     EVFRRIEGQYAAKAAIDIAVMDWIGQKLGVPLYRHFGLDPGDAPVTTFTIGIDTPEVT
                     RQKVREAEAFPVLKIKVGLDTDEATIEAVRSVTKKPLRVDANEGWKDREEAVRKINWL
                     ESQGVEFVEQPMPAAMIDDIRWVRDRVHIPIIADEACLHPADIPKLATAYDGVNIKLM
                     KCGGMLEAFRMIEIAKSLGLKTMLGCMIESSIGVTAAAHLSPLVDYADLDGNLLIAND
                     PYSGVKVVNGKLVLPDRPGLGLKRA"
     gene            complement(5131..5976)
                     /locus_tag="VLE22_26140"
     CDS             complement(5131..5976)
                     /locus_tag="VLE22_26140"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HSB17958.1"
                     /translation="MNALFVLALTMAISEAVLIHPLTDMHSGPDENADVVSQALYGTT
                     VAVLEERGEWAKIRTPDDYPGWIRLSAMRRLREGEKPYASGTLVARVENMFANLYRDP
                     DVTRHKPLLTVAFETKLEVAEEVTGDEERWLEIRLPDGRRAWVQRGDITFETMPLNVD
                     AAIALAKRFLGLPYYWGGTSTFGYDCSGFTQMLYRRMGVTIPRDSRPQARWEGFRPVG
                     RPELRPGDLLYFGSSPEKISHTGMYIGGGEFIHATTHERPVVQISRLDEAHWSGLLVA
                     CRRLK"
BASE COUNT         1138 a         2103 c         1926 g         1350 t
ORIGIN      
        1 gaaccaagcc ccgtatttct cggtacccat tttgtactcc tggtaccgcc ctgggtcacg
       61 gacacaagag gcgaagtgca gcatgtagac aaagccgaac ccacctgaga tgtgtacggt
      121 ggtcggcatg tttgccacgt cggccatgtg cgcgacccgt atggagcgga tcatgccacc
      181 gtaatagtgc aggtccggtt gcacgatgtc gacgccccgg ttggcgatca tccagcggaa
      241 gcgccactca ctgtattcct gttcgccgcc ggcgacggga attgttagcg cgtcggctac
      301 tttcttcgtg tcttcgagat gatcgaacgg gcacggctcc tcgtagtaga cagccttgat
      361 ctcctcgagc atccgcccca cttcgatggc cttcggggga tcgtaggagc tgttggaatc
      421 cgcgtgaatg tcgatcgcgt cgccgaacgt cttgcggacg agcgggatca gcgtctcggt
      481 gcgccccggc atcgcgtctg cattccggct catccggccc ccgatccgga acttcaacgc
      541 tttcgccttg gacttctcga gtaagccgcg caagttttcg atctcctgtt ccggagtcgt
      601 gtctcgccgg ccactggcca cgtagaatgc gatctcgcgg cgcacgatat cgccgaacag
      661 cgcacccatc ggctttcgtg cgatccgccc gagcatgtcg aggatggcga actccaccca
      721 cgcctgcggg ctccaaaagg cgagtccata gagcttgtag ttgctctgcc agcggtagag
      781 ttcccacagc aggttctcga gatcgcgagc gtcctttccg atgaagaacg ggatcacaag
      841 ctggttcagg atggggtgta gatgctctgc gcgcgggggg ttcgtcagcg acacgccctc
      901 ggctccatcc ttcgaccgca cgtggacgaa atactcccgg tctttcttca gcagccggat
      961 ggaatcgatc accacgggcg acgtgagccc gtcgcgtttc aacacaggcg tagtcagcgc
     1021 ccggtccaga tcttccgggc ttggcccttc cgttctcctc gcgcccttct cgggtgcgcg
     1081 cccgcacccg gaacccagcg cgacggctcc ggcaccggcc aggaacttcc tacgatccaa
     1141 tcgcattcgt atttcctccc gataacagcc attcagcgta catcggcaac gttgcccgaa
     1201 gtcaagtgtg ggaatcgagg cgggtaagaa aggaacagcc ggggcggggc gccccggctg
     1261 caaacctcaa ggactaagga aatggctcag ttgccgttct tgtaacgctt cgccatttcg
     1321 gcgagcggca acggcgcgta gtcgccggcg tgaccagcct ggccgaactc ctgcatgcgt
     1381 tgagccacga ccttcttcat agcttcgcgg gcaggcttca gatagtcgcg ggggtcgaac
     1441 ttttcgggcg tctcgataaa caccttgcgg atggcgccgg tgatggcgag ccggttatcg
     1501 gtatcgacat tgatcttgcg cacgccgttc ttgatgccca actggatttc ctcgaccgga
     1561 acgccccagg tcggcctcag cttgccgccg tactggttga tgatgtcctg caaatccttg
     1621 ggcacgctcg aggagccgtg cattacgagg tgcgtagtcg gcagccgctt gtggatctca
     1681 atcagacgat ccatctttag gactgcaccg tcgggcttgc gggtgaactt gtacgcgccg
     1741 tggctggtgc cgatggcgat ggcgagcgca tcgaggccgg tctgtttgac gaactctaca
     1801 gcctggtccg ggtcggtgat gtgctcgagg ccggagccgc ctgcgccatg gccgtcttca
     1861 atgccgccca gcacgccaat ctcgccttcc accgttacgc ccttcgcgtg tgccgtctcg
     1921 acgacttcac gcgtgacctt cacgttgtgc tcgaagctgg agggagtttt gccatcctcc
     1981 atcagcgagc cgtccatcat gacgctggtg aagcccatgt cgatagcaga cttgcaggtg
     2041 gcgacgctgt tgccgtggtc gaggtgcagc acgaccggga tctccgggta cagttcaaca
     2101 gcagcgagca tgaggtgata caggaaacgg tcctgcgagt acgagcgtgc tccgcggctg
     2161 gcctggatga taaccgggga tttcgtctcg cgggcagcct ccatgatcgc ctggatctgc
     2221 tccatgttgt tcacgttaaa agcgccgaca ccgtaaccgc ccttggcggc ctcatcaaga
     2281 agctggcgca tggacactag aggcataatt aggactcctt cctgggaact gcggatttaa
     2341 tacttcagct tatcacgatc cggatgtggg catccgaaag cggtaggttc tctccgaatc
     2401 cggcttctcc ggcacacagc ttcacacttc ccacgtgcgc cggcaacacg ctgctcctgt
     2461 actcacccgg cgaagccggc cagcttgttg agcccgtcta caaacgccct ggcgctggct
     2521 tcgatcacgt cggttgacgc gcctcggccc atgactctcc gcccggccgc ttcgaggttt
     2581 acggtcactt cacccatagc ttcggtgccg ctggtgaccg cgcgaatctc atagcgaacc
     2641 aggttggccg agctgccggc cacttgggag atcgctttgc aggcggcgtc caccgggccg
     2701 tccccgacgg cggcgccttc cctcaaatct ccccggacgc gcagcctcac agtcgccgtc
     2761 ggaatcgcgg acgtgccgct gtaaatgtgc aggtactcga gttggtaggt ctccggaacc
     2821 gggtggatct cgtcgtgcaa aatcgcgacc aggtcttcgt cgaacacttc ctgcttcttg
     2881 tcggccaggc gaaggaagcg ctgatacgtc aggtcgagtt cgccctgcga aagtgcatag
     2941 ccgagtttct ccaggcgatc acgcagaccg tgccgcccgg tccgggccgt cagcaccacc
     3001 cggctatcgg agaatccgac gtcttcgggg cgcatgatct cgtacgtctc tcgctgcttc
     3061 aggaaaccgt caacgtggat cccggagctg tgggaaaacg cgttgcctcc gacgaccgcc
     3121 ttgttcggcg gaacggccat gccgagcatg tcggcgacca ggcggctggt tcggcagaat
     3181 tcggtggtgt tgatgccggt gacgacgcgg aagtagtcgc cgcgggttcg gatcgccatc
     3241 acgacctctt caagagccgc gtttccggcc cgttcgccga ctccgttgat cgtaccctcc
     3301 acctgccgcg cgccgttggc gactccggcg agcgaattgg cgaccgccaa cccaagatcg
     3361 tcgtggcagt ggacgctgat ggtggaacgg ctgatattcg ggacgttctc cgtgatggac
     3421 cggatcagag cgccgaactg ttccgggacc gtgtagcccg tggtatcggg gatgttgacg
     3481 accgtggccc cggcgccgat ggctgcctgg agcatctcgc acaggtacgc gggctcggca
     3541 cggccggcat cctccgcata gaactcgaca tcgtcggtgt aggaacgggc gagttttacg
     3601 gcatcgatgg ccatttgcac gagcgtggct ctcttttcat cgagcgtcgt cccgtacttt
     3661 tcatcgcgga acttgcccat gatgtgaatg tccgaaacgc caatgcccgt gtggatgcgc
     3721 ggtcgtttcg ccttggccag cgcctttccg caggcctcga tgtcgctcgc cacggcgcgc
     3781 gagagggcgc aaatggccgg tccctcgatc tcggtcgaga tcaactgaac ggccacgaag
     3841 tcctcgggcg aggacgccgg atagccggcc tcgatgatgt cgacgttgag ccgggccaat
     3901 tgcctggcga tcgtcagttt ctctctgctt ccgagccgcg tgccggccgc ctgttcgccg
     3961 tccctcagcg tcgtatcgaa tatggctact ttgtcgctca tcgtaccgtc cttgggacga
     4021 ttctatcgct cgttagcgcg tgcggggctg ggcctcacgc ccgcttcagg cccagacccg
     4081 ggcggtcggg cagaaccagc tttccgttta ccaccttcac gcccgagtaa gggtcgttgg
     4141 cgatcagcag gtttccgtcg aggtcggcat agtccactaa tggggaaagg tgcgccgccg
     4201 cggtcacgcc gatcgagctt tcaatcatgc agcccagcat cgtcttcaag cccagcgact
     4261 ttgcgatctc gatcatccgg aacgcttcca gcattccgcc gcacttcatc agcttgatgt
     4321 tgacgccgtc gtaggcggtt gcgagcttcg ggatgtccgc cgggtgcagg cacgcctcgt
     4381 ccgcgatgat cgggatgtgg acacggtccc gcacccatcg gatatcgtcg atcatcgccg
     4441 ccggcatcgg ctgctcgacg aactccactc cctgcgactc cagccagttg atcttccgga
     4501 ccgcctcttc cctgtctttc cagccctcgt tagcgtcgac gcgcagcggc ttcttcgtta
     4561 cgctgcgcac ggcttcgatg gtcgcttcgt cggtatcgag accgaccttg atcttgagca
     4621 cgggaaacgc ttcggcctcc cgcaccttct gccgtgtaac ttccggcgtg tcgataccga
     4681 tcgtgaacgt agtcaccggg gcatctccag ggtccagccc aaaatggcgg tatagcggga
     4741 cgccgagctt ctgtccgatc cagtccatca cggcgatgtc gatcgcggcc ttggcggcgt
     4801 actgtccctc gatgcgccgg aacacctccg ccagaagctt tgaaaaatac cagggatcgg
     4861 cggaacggag caggtcggcg accgactgca ccgctttacg ggcggagtcg gcgttctctt
     4921 tgtaacggac gatcggcgcc gcctccccga agcccgtcac cccgtcgcgc ttgaatcgca
     4981 ggtgcaggtt gtcgcgatag tcgctcgatg acatcaccgt cgtccacgtg tgccgaagct
     5041 tcagacgcac gacctccgta cgaacttcgg cggactctgc ctgccccggc cacgcagcca
     5101 ggcccgccat gccgatcaga tcccgccgcc tcatttcaac ctccggcacg ccaccagcaa
     5161 cccgctccag tgcgcctcgt cgaggcggct gatctggacc accggacgct cgtgtgttgt
     5221 cgcgtgaatg aattctcccc cgccgatgta cattcccgtg tgactgattt tctccggcga
     5281 actcccgaag tacaacaggt ctcccggccg gagctcgggg cggccgacag gccggaatcc
     5341 ttcccaacgc gcttgcggcc ggctgtcccg tggaatggta acgcccatcc gccggtaaag
     5401 catttgcgtg aagccggagc agtcataccc gaaggtcgag gttccgcccc agtaatatgg
     5461 caagccgagg aaccgctttg cgagcgcgat ggcggcgtcc acgttcaatg gcattgtctc
     5521 gaacgttatg tcccctcgtt gcacccacgc ccgccgtcca tccggcagcc ggatctcaag
     5581 ccaccgttcc tcgtctccgg tgacctcttc ggcgacttcc agtttcgtct cgaacgcaac
     5641 cgtcaggagc ggcttgtgcc gggtaacatc cgggtcacgg tacaggtttg cgaacatgtt
     5701 ttcgacgcgc gccaccaggg taccggatgc atatggtttc tctccctccc gcaaccggcg
     5761 catcgccgac aggcggatcc agcccgggta gtcgtccggc gtccgtatct tcgcccactc
     5821 tccccgttct tcgagaaccg ccaccgttgt gccgtaaagc gcctgggaga cgacatcggc
     5881 attctcgtcc ggaccggaat gcatgtccgt caacggatga atcagtaccg cctcggaaat
     5941 ggccatagtc agcgctaaga cgaacagagc attcattctc agactcactg ccgggggacc
     6001 ggctgttcac atattaacct tcgatagcgg gagtacatca cccattttca gtattgaccc
     6061 acggccgact tcagggttaa gtagactaca ggcggtgatt ggcccggccg actttaatag
     6121 tgggtaccgg cgagtctttg gccggcgcca atcgccgcgc tacaacacag acaccagcgg
     6181 gaggggaggc acgccgccct acaagagccg cgagactccc ttccgttcca agcgggattg
     6241 gcaggctggc acgctcagga ggagtaggcg agggtagacg ccagccagcc tgcatgtttt
     6301 tctcaggtat gcgcaatggc aagagcgctt gagttgttga aagatcgttg gctggaaatc
     6361 ctgctgctgg ctctctccat cggaatctcc gctccgttaa cccgatagcc tgcgctcacc
     6421 cgctctattt ctttcttccg cccgcgagca tcccatcgat caacgtgaat agcgccttcc
     6481 cctcgaagat cgcctggttc gccgggctgt atccgta
//
LOCUS       DATIFQ010000519        28263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1709806, whole genome shotgun sequence.
ACCESSION   DATIFQ010000519 DATIFQ010000000
VERSION     DATIFQ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 28263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28263
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709806"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1895)
                     /locus_tag="VLE22_26145"
     CDS             complement(180..1895)
                     /locus_tag="VLE22_26145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSB17959.1"
                     /translation="MSSLCLLLFANAPARLLSLSFADLLIIAIYFVAVLVIGFYLKRH
                     TKTGEDFFLAGREMTAWVAGLSFLAANLGSLELMGWAAAAYQYGILATHWYWIGAIPA
                     MLFLGIVMMPFYYISKTHSVPGYLMLRFGEPSRALSAVSFAFMTVLMSGINMYSMALV
                     MKVVLGWDIHFSIWASSLTVAVYVALGGLLSAIFNEVLQFVLIWLGALLIPIVGLIEA
                     GGWNGMVERISRNFPGQNFTNLWSTMGSFTDNPMGIHWTGIVLGLGWVISFGYWTTDF
                     LVVQRVLAAKDLRSAKMAPIIGAGFKMMVPFIVILPGLLGLALLPIKLVGEGEAIATG
                     GHSYNEVLPLMLARYLGPGLLGLGITALIAGFMSGMAGNVSAFATVWTYDIYRALIKK
                     EAADSHYVKMGRWCTIIGVLISIGTAYLVMEFKSIMDYVQALFSFFIAPLFGTVLLGM
                     LWRRATPAGGFWGLLSGTVSSISMWAWVKMDPGALAYIALSRHAKDMAENMYRALWSW
                     IICVVVTVVVSLVTKPKPAEELKGLVYGLTEVPSERHLPIYQRPIFWAVVVGTVFAVL
                     NIIFW"
     gene            2031..2357
                     /locus_tag="VLE22_26150"
     CDS             2031..2357
                     /locus_tag="VLE22_26150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17960.1"
                     /translation="MANPEKHGKNPVREWFEGEEGTVPANPRSSHLVGSYIKGSKLLA
                     KKPRPVTAARAQSQTPSKPEPALLREPGPPAKANAKPSQAASWEHQKKLGRLPNLRAP
                     YQYKSR"
     gene            complement(2364..3014)
                     /locus_tag="VLE22_26155"
     CDS             complement(2364..3014)
                     /locus_tag="VLE22_26155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSB17961.1"
                     /translation="MSEIRVLLADDHGVVRKGLRYLLQKSPEMKVVGEAADGREAVAM
                     AEELNPDVVVMDIAMPQLNGIDATAQIVKKNPRTGVIMLSMYSDETYLVRALGVGAKG
                     YLLKDSTESDLVRAVQAVSQGRPFFSPAIAQTLLDDYMRRLRQENLQDSYDLLTDREK
                     EVLQLLAEGKSNKEVATLLNLSLYTVETHRSHLMQKLNLHNTAEIVLYAVRKKIIS"
     gene            complement(3011..4354)
                     /locus_tag="VLE22_26160"
     CDS             complement(3011..4354)
                     /locus_tag="VLE22_26160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSB17962.1"
                     /translation="MRTRTWPILIFGFGSLVFLIALAGIGASRRARQIQSEIMNIHAT
                     DREITAALTGIGSQIHISGILARDFLLDPSHLTAEAHREHLREIRNSMGEQLEKLERL
                     LGAEDAGVLRELRREIDAYWDSLDPIFEWTPQQKIALSSLFLRRQVLPRRDAVLNMAH
                     EIQKLNASNLQRQQQLLRRSQAAFRRYVAWMIAFVVLLGILVAVLSILRVSRLERRTE
                     EQQRITERAEGELRRLSQQLVRAQETERKSISRELHDEVGQMLTGLRMELANLEDDRH
                     TPGERFQSRLADVKHLAEQTLVSVRNIAMGLRPSMLDDLGLGAAVEWQGREFSRRSGI
                     PVTVQIDGTLDGLPDSHRTCVFRVAQEALTNSVRHSQARTIRVAIHGRPDAVFLTVQD
                     DGVGLDASESSGPGLGLIGIQERVRELGGTVTIHSLPNRGTSLRVEIPLGKEVPC"
     gene            4449..5246
                     /locus_tag="VLE22_26165"
     CDS             4449..5246
                     /locus_tag="VLE22_26165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HSB17963.1"
                     /translation="MIVVFTDLDGTLLDHETYSWEPARPALDALARRRVPLILCTSKP
                     RAEIEPLRSALGNRHPFIVENGGAAFLPQGYFPFAVPGAASRDGYEVIEFGNRYVELV
                     EALRLAAHTAGVPVRGFSVMTAAEISERSGLSPEKSRLAKLREYDEPFYILDPSRERI
                     LLREIEVLGKRWTRGDRFFHITGSNDKATAVRRLMELYRQAHGTVTTIGLGDAPNDAE
                     FLAVVDNPVLIRSRFSAALQALVPNGRLTARPGPYGWNEAILEMVPE"
     gene            complement(5226..5930)
                     /locus_tag="VLE22_26170"
     CDS             complement(5226..5930)
                     /locus_tag="VLE22_26170"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17964.1"
                     /translation="MIETMQQVLEGVLARLKYQATTYLPSLVAALIILLGAYIAALLA
                     RWVLNRIFKGIAVDRFLRRTGLAFMLDRSGRLRATRLVSESVYWLILLGGFLTGLSVF
                     NTDLTTQITQSFVFLLPKLVVAGLILLAGVWLSQFLGRSTLVWAVNEGVPSPRKLAGA
                     VRVAIMFVAVVVAADQLNFARSVFLAAFIILMGGAVFAASLALGLGGRDSARRYFQEQ
                     SEAKEERGERSLWNHL"
     gene            complement(5934..7277)
                     /locus_tag="VLE22_26175"
     CDS             complement(5934..7277)
                     /locus_tag="VLE22_26175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall biosynthesis glycosyltransferase"
                     /protein_id="HSB17965.1"
                     /translation="MAENQLPLDALRAAEKIRKADVVIGIPSYNNARTIGHVTRAACA
                     GLAKYFPDLTGVIVNSDGGSKDGTAQAVVSAGMEDANLLLLSTPLTGVHRLSFPYHGI
                     PGKGSAFRLIFQMAERLEARACAVVDSDLRSITPEWFDLLLRPVLFAGYDFVAPYYHR
                     HKYDGTITNSIVYPLTRALYGHRVRQPIGGEFGVSARLLSRYLSREDWETDIARYGID
                     IWMTTIALAEGFRVCQSFLGAKLHDAKDPGADLSAMLHQVVGSVFLLMQEYEPVWRNR
                     SGSEAVELFGFRFDVGLDPIEVNLDRMLNAFRHGCEELGEVWSIALQPSTFSEIRKLA
                     EQAHPGATSFHIDDDLWTRVVLDFACAHRKARLGRGHLLRSLTPLYLARVASFVIETT
                     SLLSAQVEQKIEELCLCFERMKPYLIAHWDAEPAPAAEKPVPKKSGVVERANLEV"
     gene            complement(7288..8517)
                     /locus_tag="VLE22_26180"
     CDS             complement(7288..8517)
                     /locus_tag="VLE22_26180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17966.1"
                     /translation="MSDFYQTGAVTTLHRLRPNSIERLEGDLERFSRNRPIGLVLPAL
                     YSEFQTPAMQRIVPEVKQVRYLQRIVVALGRASRQEFDHARSYFEDFYTPVTLIWIDS
                     ERIQKLFQILEERGLSAGGDGKGRSCWMAYGYMLAMGDCDVIALHDCDIVNYDRQLLA
                     RLCYPVTNPNLGFEFCKGYYARVTDRMHGRVTRLFVTPLVRALDSMAPGTDFLRFLDS
                     FRYPLAGEFAMDANLARVNRIPGDWGLEVGVLAEVYRNCATARICQVDLTDNYEHKHQ
                     TLSAEDATKGLRRMAADIAKSLFRTMAAEGIVFTADHFRSLGVRYVRMAQDTIARYYA
                     DAMLNGLKFDRHGEEMAVAAFAKSLNQAAAEYIEDPLGLPLIPNWNRVLAAIPEYFDL
                     LRDAVDKDMKLVVGRGA"
     gene            complement(8606..9841)
                     /locus_tag="VLE22_26185"
     CDS             complement(8606..9841)
                     /locus_tag="VLE22_26185"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSB17967.1"
                     /translation="MTHFDLSAAFSLLLATGPEAVKIPLSMLIVFGSAKLMAELFERL
                     RQPAIVGEIVAGILIGPSVLGWIEPAELITALADLGVMFLLFRVGLEVKSSELMRVGG
                     TALAVAILGVIVPFALGWGIFQFWGEPQLESIFVGAAMVATSVGITAQVLASGGWLQA
                     KASRIVLAAAVIDDVLGLLVLALVSSMAKGSVNLLELALTGAVAIAFTIIVAKWGTRT
                     MERVVPRVQQRLHICEAQFAFAMVLLFGLSLLAVYAGVAAIIGAFLAGMALSESVSHR
                     VHDLAHGVTELLVPFFLASIGLHVDLSVFSQSSILALALLIVVAAVLAKLVGCGMGAI
                     RLGRADAFRVGVGMVPRGEVGMVVAQIGLGLGVLGEHVYGVVVFMAVATTLAAPPLLR
                     LAYRNLAPVVPEEAPLRLG"
     gene            complement(9860..10363)
                     /locus_tag="VLE22_26190"
     CDS             complement(9860..10363)
                     /locus_tag="VLE22_26190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HSB17968.1"
                     /translation="MDLHDSIGFSMLHYPIFLALSGRPVLVAGAGKVALRKVTGLLET
                     GARVTVVAPEALPEFERLGAHLRKRRFRLSDLNGMALAFAATNDRKVNRAVALEAKRR
                     GIPVNVADCPEECDFLVPARVRSGEVQIAVSTGGHSPRLAAELRRKLESVLAHGTRQS
                     DDSQTLL"
     gene            10362..11663
                     /gene="der"
                     /locus_tag="VLE22_26195"
     CDS             10362..11663
                     /gene="der"
                     /locus_tag="VLE22_26195"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HSB17969.1"
                     /translation="MAESPRLPTVVIVGRPNVGKSTLFNCILGRRRSIVGDESGITRD
                     RIHGEAVHRGRHFELIDTGGILPNEAELIPSEIFRQARVALERAAHIILLIDGRTEIT
                     ATDRELAQLLHRLGKPVALAVNKIDTAARDVLAHEFHVLGISDLFPVSAEHRLGIDDL
                     LDHVTTGFDLTEIQEAEKAPIKVAIIGRPNVGKSTLLNTLVGEDRAIVSPVAGTTRDA
                     VDESVVIDGCQFVFVDTAGIRRKGKTKLMAEKISVVMARRHIRMANVVLLVLDATEGV
                     VGLDANIAGYAHEDGRAIILCVNKWDVSPDKSTKTFTTKVRDEMKYLEYAPVAFVSAK
                     TGAGVHRLFPLIRELYDAASQRVSTGELNRFLETLHIESEQKVQYITQPSVRPPTFVL
                     FTDRPGKLHFSAERYLINRLRKQFGFRGTPIVIKTKAKQRR"
     gene            complement(11674..12345)
                     /locus_tag="VLE22_26200"
     CDS             complement(11674..12345)
                     /locus_tag="VLE22_26200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB17970.1"
                     /translation="MRRTLGLLLLSTTGWCQVAKEANREYETEEGRARMAGVLESPSR
                     LENLRPAELMQRLEIRPGSTVVDLGTGTGTLLRDLSKAVGVEGRVIAEDIHQDFLDLA
                     RERVKEAARHNVEFVLGTETDPKLPASAADLVIVLDVYHHFDYPERMLAGIKQSLRPG
                     GRLAIVEYHKKRGAMERDPDFALTHIRASAEQVVQEIEAAGYELLWRRDHAPEKQYIA
                     MFRVK"
     gene            complement(12411..13730)
                     /locus_tag="VLE22_26205"
     CDS             complement(12411..13730)
                     /locus_tag="VLE22_26205"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HSB17971.1"
                     /translation="MLPDYLSKAVPNSPSNRAPWYINTAPSYAGIFLWVVFYMEIAKG
                     TIDRAGLGLCLLALVVAGLISYALFYRVPAMLGMQTGYPLYVVGSSTFGTTGGYLMPG
                     LLMGLLQIGWFSVNTLVSSTFILSAFGSKAGPGTLPFALVAIVWAYGMAYIGVKGIQY
                     VAKFALYLNLIPLLLIILVFFRTAVGIGQHVPADPNPYVGFMLLVQIVVGFFATAGAA
                     GADFGMNSRNANDVKMGGLVGITLAILIAGGLPLLSVAGARVLYGVQGFTYDAVISGI
                     GGFLAAAMFLVFAIASAPPACFCAFIAGNSFATMIPGVRRMASTMVGVTVALVLAITG
                     AASNLIGLFTIVGASFGPICGAMAADYLLSGNKWAGPREGVNWAGYGAWAVGFLIGIL
                     PFLPVAEETKPYLQPAAVYGFLAGFLVYALLAKAGLQPKVTPLPTKS"
     gene            complement(13815..14360)
                     /locus_tag="VLE22_26210"
     CDS             complement(13815..14360)
                     /locus_tag="VLE22_26210"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HSB17972.1"
                     /translation="MLRVTSSLKLDGIEAALRQAAQRRGASVVSVMHLGHLIRNKEPG
                     AAKDAIVFSLCHPDLSAALLGADLRLSAMLPCRLAAFEEGGSVTLTAISPSEAGRALS
                     RPDLETLFLLLEATLQDIMNEVSQPAAAAAHAPSVSRHRDVGATEEQVNARASIPQRL
                     DCRGTKVEELAGTGEHDGSGG"
     gene            complement(14402..15064)
                     /locus_tag="VLE22_26215"
     CDS             complement(14402..15064)
                     /locus_tag="VLE22_26215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17973.1"
                     /translation="MWGGVGAPPADARHDVLHLLRMALPESIRVKLSSEEAGAISITA
                     VVVREMAARELIELMLGIAGKDPGRIHELLLRGTLVSGGTRFRWQGWEADRESIERIL
                     ATFPDSEPGLPFAPERCLRVVLRGPGCRIDLPREVASKKRLLRRRSYWDVLMEAAESA
                     AVHYLEYSHREHADCYRMEVSPQAAARLREQAALVGYSGLEAQIRSTALEIVDLFVER
                     AR"
     gene            15291..15497
                     /locus_tag="VLE22_26220"
     CDS             15291..15497
                     /locus_tag="VLE22_26220"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HSB17974.1"
                     /translation="MKEKGTVKWFNAAKGYGFIQRENGEDVFVHYSAIQTDGYRTLEE
                     GSKVEFEVTQGPKGLAAANVTMDA"
     gene            15613..15834
                     /gene="infA"
                     /locus_tag="VLE22_26225"
     CDS             15613..15834
                     /gene="infA"
                     /locus_tag="VLE22_26225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HSB17975.1"
                     /translation="MMSKEDCIEVTGVVVEKFPSGLFSVQLDQDRVILAHLAGKLRRN
                     RIRVLAGDRVTLELSPYDLTKGRITYRHK"
     gene            15860..16963
                     /gene="wecB"
                     /locus_tag="VLE22_26230"
     CDS             15860..16963
                     /gene="wecB"
                     /locus_tag="VLE22_26230"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HSB17976.1"
                     /translation="MFVFGTRPEAIKLCPLVVYLLASRRFTVRVCVTAQHRQLLDQVL
                     QAFGVRPDYDLGAMLPDQTLFQSTSRILSALEPVLGTERPDLVLVQGDTTTTLCGALA
                     AFYARAAVGHVEAGLRTHDTARPFPEEINRVLVGRLATLHFAATEGAAANLRAEGVAP
                     ERISVTGNTGIDAVLYVRDALEQGRLHGAGLPPLDPSRRLLVVTAHRRESFGPGFENI
                     CSALERLARRPDVQIVYPVHPNPNVREPVFRRLSEFANVLLTEPLDYVTFVDLMRRSS
                     ILLTDSGGIQEEGPSFGKPILVMRDKTERPEAVEAGTVRLVGADENRIVEQASRLLDS
                     PDEYQAMSRVHNPYGDGRACERITAVIDSFFDR"
     gene            17064..18092
                     /locus_tag="VLE22_26235"
     CDS             17064..18092
                     /locus_tag="VLE22_26235"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSB17977.1"
                     /translation="MKNGAGTRRAKRNLVQGSSGVAEMLASRVVGQSPALDAIVPCVE
                     MYQAGLSPEGRPAGVFLLLGPTGTGKTHTVEALADVLHGSEKCLLRIDCGEFQLEHEV
                     AKLIGAPPGYLGHRETQPLLTQQKLNMAASEGCGLSLVLFDEIEKAAPSLTRLLLGIL
                     DRGTLRLGDNAVVNFERSLIFLTSNLGARGMMRELRPVFGFEAGVPKERASVDRKLAN
                     IGREAVKRKFSPEFVNRIDHVITYRPLDGDSLSRILDRHISDFQRHVNSRLGPDGFGI
                     EVPSETRRFLLRKGTSAEYGARELKRTLHRHLTQPLAALVAGGRIEPGSSVRIEIDED
                     EDDVFIRY"
     gene            18070..18939
                     /gene="rsmI"
                     /locus_tag="VLE22_26240"
     CDS             18070..18939
                     /gene="rsmI"
                     /locus_tag="VLE22_26240"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HSB17978.1"
                     /translation="MTCSSDTKGVAQPGTLYLVATPIGNLEDITYRAVRVLREVDVIA
                     CEDTRHTRKLLDRYGISTPTVSYHEHNEAERAEALLERLLHGTSVALVSDAGTPLVSD
                     PGYRLVNGAIEHGVPVEPLPGPSAVLAALAASGLPTDAFHFGGFLPSKSTQRRKVLET
                     LKDEQATLAFYEAPHRIIESLADIEAVLGPRRVVIAREITKVHQEFLRGLPAELRGIL
                     EAWPAIKGEITVLIAKGTSPEAGDETPLPEAVAELVGSGIPRMDAIKTVARRRGLSKR
                     EVYDAVENSRKHG"
     gene            19090..21303
                     /locus_tag="VLE22_26245"
     CDS             19090..21303
                     /locus_tag="VLE22_26245"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HSB17979.1"
                     /translation="MRKLWITFAIVVVASFAVLGWIGTRIFQEMPPIPDRVVTTDGQE
                     VFAGGEIGRGQNVWQSLGGMEVGSIWGHGSYVAPDWTADWLHREAMFILDEWGRKEFG
                     KSYDQLPPESAAQLQGRLEQMYRTNTYDPATKTLTIDPVRARAFEASLVHYSDVFMNG
                     NTAYSIPPGTVRDAGRMRQFASFMFWTAWAASANRPGDQVSYTHNWPHEPLVGNKPTG
                     ESVMWTGVSIIMLLAGISGLVWWYAAQAHEEQHGPLPPVDPLGAWRASASQFATLKYF
                     WVVAVLILVQMLMGVVTAHYGVEGDGFYGFPLSKWLPYSVSRTWHVQLGIFWIATAWL
                     AAGLFIGPLVSGTEPKGQKLGVNVLFLALLVVVAGSLTGEWLSVHNKLSDATSFLWGH
                     QGYEYLDLGRGWQALLFVGLLIWLFLVVRSIRPALKQPGEQRPLLWIFLMSAAAIGLF
                     YGAGLNWGQHTHLSMVEYWRWWVVHLWVEGFFEVFATTVIAFFFTRLHLIRPALAAKA
                     ALLSATIYLAGGIIGTCHHLYFSGTPTVALAWGSVFSALEVVPLTLVAYTAVDDLRRG
                     RLTPWAQRYKWPVYFFVAVAFWNMVGAGLFGFMINPPIALYFMQGLNTTPVHGHAALF
                     GVYGMLGIGLMLVCLRALLPDLQWKDKWLRFAFWAMNIGLLTMCVGSLLPVGLLQTWA
                     SVDQGYWYARSKEFLGTPLMQTLRWLRVPGDTIFALGEFAIVAFVLTIRPGKSRA"
     gene            complement(21329..21610)
                     /locus_tag="VLE22_26250"
     CDS             complement(21329..21610)
                     /locus_tag="VLE22_26250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17980.1"
                     /translation="MEIPIGQEVTFIDPEYSSGCWVGFKGIVRANPFYEICRSQQDVE
                     IQGNWELLRGEARDSHWVMAYGDHLAAVGYAARKLGVRWVDIAKPAVSS"
     gene            21748..22596
                     /locus_tag="VLE22_26255"
     CDS             21748..22596
                     /locus_tag="VLE22_26255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17981.1"
                     /translation="MMRVAIFGTILAAVPILAQMPQNPSPMVEHTRAHRRLTQETPPG
                     RREAIEPGTLFLPDRLRLKNGIPLLVFFHGGAWIPEVAAARHRMAVISVQIGAGSGVY
                     ARAFADPAGFPALLAEAERRAGVRIGRVMLGGWSAGCGAIREILKSPESYARVDATLM
                     IDGIHSGYVNGKPGPLESEIETDALQIWLQLARDAIAGRKRAIITHSEIFPGTFASTT
                     ETADFIIRQLGFKRRAVLKWGPMGTQQLSEVRAGRFLLIGYAGNTAPDHVDQLHALPE
                     YLKWLK"
     gene            22584..23207
                     /locus_tag="VLE22_26260"
     CDS             22584..23207
                     /locus_tag="VLE22_26260"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HSB17982.1"
                     /translation="MAQVNPDRRRHLVLLAIVAAALVWSAIRPRDYFTWFLEVAPVFV
                     GFVLIAATYRRFPLTTFLYSLLAAHSLILMVGGHYTYAEVPLFNRLRDIGIFSRNNYD
                     KVGHLAQGFFPVILAREILIRTSPLAGSRWLGFLSASVCLAFSALYEIMEWWVSVLGG
                     SRGDAFLGTQGYIWDTQSDMFLCLVGSITALLLLAQAHDRALRRLPG"
     gene            complement(23204..25465)
                     /locus_tag="VLE22_26265"
     CDS             complement(23204..25465)
                     /locus_tag="VLE22_26265"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB17983.1"
                     /translation="MEPGSAGGARLRFGLFELDLAAGELRKRGRKIRLQEQPLQVLVL
                     LLRNPGAVITREALQKALWPEGTFIEFEHGVNTAIKKVRRALGDSADNPRFVETLPRK
                     GYRFIAPVAEVKGPESAAALESDAVPPSTPWWRSVTGVAVAAGLLVCVALSVVWWFTR
                     ETGPASTPQSQYRMKSLTSDSGFTGYPAFSPDGKLVAYASDRAGGGNLDIWVQQLDSG
                     ATIRLTDHETADHSPAFSADSSRIFFRSERDGGGIYVVPTLGGDSRLVVKQGLAPEIS
                     PDGSQIAYFVGIKGALLGTQMYVASVNGGQARQLVPDFPAAAKPMWTRDGKHLMFIGV
                     HPTYGFDVWVAPLDGRPVVRTGVVEILKNQRMGLATLDGYLANTEDLVFPGQSGDSGS
                     LLFSGRSGDSSNLCRIWIDPKTWKAKGPVERLTFGVGEGDASAGPGGRIVFMSASRRL
                     TLWSVKVDANRGEASEEPKQVTDAGGNDSACDVSPDARRLVFRSDRTRSPEIWTKDLI
                     TGAERALTDAPRVFSSMPKIAPDGKTVAFQVIEDNKRSVYTLSIDSGLATKVCLDCGP
                     PDTWSPDGKRITYLRPDVPRATIHLLDLGTGRQSLLLAHPELPVYVSRFSPDGQWVSF
                     KVDLDMHRTRIFIARVKEDSASPVNEWIAVTEGGAWDDLPLWAPNNRLIYFTSDRDGY
                     RCIWARRLNPVTKQPLGEVFPVGHFHRIQHSLSAISLSEMTLTLGNNQLFFPMAELRG
                     NVWMMEPRNDSRR"
     gene            25670..26314
                     /locus_tag="VLE22_26270"
     CDS             25670..26314
                     /locus_tag="VLE22_26270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17984.1"
                     /translation="MKVLVAGILLAGVALPLHGNKGEEPLRLRVFMYNYAETSQSLVD
                     RARRETSMIFRRAGVEIEWVQCTLSMRNAPQQLCSMRRGPANLELRLLPRRMAGRMRL
                     GSNAAGFALQPLDGVPGVLANVFPDHAADLATNSGLDRGVMLGHLIAHELGHLLLGIG
                     SHSTGGMMAAPWGRRELSWAMKGCLTFSRPEAEKIRSRLLLRSAGMGTPNAAIR"
     gene            26371..27684
                     /locus_tag="VLE22_26275"
     CDS             26371..27684
                     /locus_tag="VLE22_26275"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HSB17985.1"
                     /translation="MAEVAARTREPVIGPVLRTLRPIIYLTAFLWRRLLFRTTFIGVT
                     GSLGKTTAKECLGTILASQYLTYRTRLNQNAPLGVALNILHVRPWHRFAVIEAATGQP
                     GMMRSSARMLRPDVVVVLNLKRTHTTAFRTLAEHAAEKERLVRAVRPGGLAVLNADDT
                     RVASLADGKKLRVTTFGTNEGCDVVVARVAGTWPERLSFEVRSGLETLAVRTRLVGVH
                     WVNSALAALATAHALGFRMADGVEALHAVEPFPGRCQPARVPSGAIFLRDDYNSSIDS
                     ISPSLSVLRDAEAERRVLVMTDVSDLGVNRRGRLRHLAPEICASADSAVIIGEAAEFG
                     KRRLVEAGMAPENVHAFDDLQPAAEFLRSELRDGDLVLLKGRTTDHVARVFFAQLGGV
                     RCWKPYCRKTILCDHCPDLGTPPEQQRRASLVSLPQRTGEVTRLP"
     gene            complement(27668..>28263)
                     /locus_tag="VLE22_26280"
     CDS             complement(27668..>28263)
                     /locus_tag="VLE22_26280"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB17986.1"
                     /translation="GRTETLIPLVRKTFGDAIDIHADSNSSYDPPKAIEMGQMLEEIK
                     AVYYEEPCPFDHLEDTKKVADALTIPVAGGEQEYSEWRFRWMIANRGVDIVQPDLHYY
                     GGMIRSIRVAHMADVANMPTTVHISGGFGFVYMLHFASCVRDPGRYQEYKMGTEKYGA
                     WFDSPITVKDGKMSVPTGPGVGIKDIKGLLQGATAVV"
BASE COUNT         5317 a         9151 c         8476 g         5319 t
ORIGIN      
        1 atcagcaggg cgccccacca caactcggtg tgaaggttcg ccatcacgac ttcgcgctcg
       61 ggcgggctgg tgagtccgta gaggccgaag ccgagaatga tgacgccata aatcagcagc
      121 agaacgccga tgaagaacca gatcgagatg aagtgccggc ctttgctcat gttccgctcc
      181 taccagaaga taatgttcag caccgcgaac actgtaccca cgaccaccgc ccagaagatt
      241 gggcgctggt aaatcggcag gtgccgttcg gacggtactt ccgtcagccc gtaaacgagg
      301 cctttcagtt cctcggccgg tttcggcttc gttaccaggc tcacgacgac cgtcacgacc
      361 acgcagatga tccaggacca tagcgcccgg tacatgttct cggccatgtc cttggcgtgg
      421 cgggacaacg cgatgtaggc cagcgcgccg gggtccatct tcacccaggc ccacatgctg
      481 atggaggaaa ccgtgcccga cagcaggccc cagaagccgc ccgctggagt agcgcgccgc
      541 cagagcattc cgagcagcac cgtgccgaac aatggcgcga taaagaagct gaacagcgcc
      601 tgcacgtagt ccatgatgct cttgaattcc atcaccaggt acgcggtgcc gatgcttatt
      661 agcacgccga tgatggtgca ccagcggccc atcttcacgt agtggctgtc ggcggcttcc
      721 ttcttgatca gcgcgcggta gatgtcatac gtccagacag tggcgaacgc gctcacgttg
      781 cctgccatgc cggacatgaa gccggcaatc agcgctgtga ttcccaggcc gagcagaccc
      841 ggcccgaggt agcgcgcaag catgagcggc agaacctcgt tgtagctgtg gccgccggtg
      901 gcgatcgcct cgccctctcc cacgagcttg atcggcagca aggccagccc cagcagcccc
      961 ggcagaatca cgatgaacgg caccatcatc ttgaagcctg ctccgatgat cggcgccatc
     1021 ttcgccgacc gcaaatcttt ggccgccaga actcgctgca ccaccaggaa gtccgtcgtc
     1081 cagtagccga acgagattac ccaacccaac ccgagcacga ttcccgtcca gtggatgccc
     1141 atcgggttgt cggtgaacga tcccatcgtg ctccagaggt tggtgaaatt ctgacctggg
     1201 aagtttcgcg agatgcgctc gaccatcccg ttccatccgc ccgcttcgat cagcccgacg
     1261 atcggaatca gcagcgctcc gagccagatc agcacgaact ggagcacttc gttgaaaatc
     1321 gccgaaagca gcccgccgag cgccacgtag accgccaccg tgagcgacga ggcccagatg
     1381 ctaaagtgga tgtcccaccc gagcaccact ttcatcacca gcgccatcga gtacatgttg
     1441 atgccgctca tcagcacagt catgaaggcg aacgaaacgg cggacagcgc ccggctcggc
     1501 tcgccgaatc tcagcatcag gtatcccggc acggagtgcg tcttcgagat gtagtagaac
     1561 ggcatcatca caatgccgag gaagagcatt gcggggatcg cgccgatcca gtaccagtgc
     1621 gtcgccagga tgccgtactg atacgcggcg gcggcccagc ccatgagctc gagtgaaccc
     1681 aggttggcgg ccagaaaact caacccggca acccaggccg tcatttctcg cccggcgaga
     1741 aaaaaatctt cgccggtctt cgtgtgccgt ttcaggtaga agccaatgac cagaacggcc
     1801 acgaagtaga tcgcgatgat gagcaggtcg gcgaaactca gggacaggag ccgtgccgga
     1861 gcgttcgcaa acaacagcag acaaagtgag gacatgccgt tcaagatcca tcaattctac
     1921 agggacttcc gcgggttgtc gatgatgttc gatttcgctt gaaacccgcc ctgttcgagc
     1981 ctacaatcga accagacgaa tggactaccc caagtccaag gaggaggctg atggcgaatc
     2041 ctgagaagca tgggaagaac cctgtccggg agtggtttga aggcgaggag ggaacggtac
     2101 cggcgaatcc tcgcagttcg caccttgtgg gctcctacat taagggttcc aagctgctgg
     2161 cgaagaagcc caggccggtg actgccgccc gggcgcagag tcagacgccg tcaaaacccg
     2221 aaccggctct tctccgagag cccgggccgc cggccaaggc caacgcgaag ccgtcgcagg
     2281 ccgcgtcgtg ggagcatcag aagaaactgg ggcgcttgcc gaatctgcgt gcgccctacc
     2341 agtacaagag ccgttgaatt ccgtcaggag atgatcttct tgcgcacggc gtagagaacg
     2401 atttccgccg tgttgtgcag gttcagcttc tgcatcaggt gggagcggtg cgtctccacc
     2461 gtgtataggc tgaggttgag gagtgtcgcg acttccttgt tcgacttccc ttccgcgagc
     2521 aactggagaa cctccttctc gcggtccgtc agcagatcgt aggagtcctg aaggttctcc
     2581 tgccggaggc gtcgcatgta gtcatcgagc agcgtctgcg cgatcgcggg gctgaagaat
     2641 gggcgcccct gcgagaccgc ctgcaccgcg cgcacgagat cgctctcggt tgaatccttc
     2701 aaaaggtagc ccttcgcccc gacgccgagc gcccgcacga ggtatgtttc gtcggagtac
     2761 atgctgagca tgatcacgcc ggttcgcggg ttctttttca caatctgtgc ggtggcgtca
     2821 atgccgttca actgcggcat ggcgatgtcc atcaccacta cgtcagggtt gagttcctcc
     2881 gccatcgcca ccgcctcccg gccgtcggcc gcttcgccga ccactttcat ttccggcgac
     2941 ttctgcagca gatatcggag gcccttgcgg accacaccgt ggtcgtcggc cagcagaaca
     3001 cggatctcgc tcaacagggc acctcctttc ccagcgggat ttccactcgc agagaagtgc
     3061 cgcggttggg cagcgagtga atcgtcaccg tgcctccaag ctcgcgcacg cgttcctgga
     3121 tgccaatcag cccgagtccc ggccccgagg attccgatgc atcgagaccg acgccgtcgt
     3181 cctggacggt gagaaacacc gcgtccggcc gtccgtgaat ggcgacgcgg atggtgcggg
     3241 cctgcgaatg ccgcacgctg ttcgtcagcg cttcctgcgc aacgcggaac acacatgtcc
     3301 gatggctgtc cggaagcccg tcgagagttc cgtcgatctg gaccgtgacc gggatcccgc
     3361 tccggcgact gaactctctg ccttgccact cgaccgccgc gccgagcccg agatcgtcca
     3421 gcatcgacgg tcgcagcccc atggcgatgt tccgcaccga gacgagggtt tgttcggcaa
     3481 ggtgcttcac atccgcgagg cgggactgga accgttcccc tggcgtgtgc cggtcgtcct
     3541 ccaggttggc cagttccatt cggagtcctg tgagcatctg accgacctcg tcgtgcaact
     3601 cgcgggaaat ggatttccgt tccgtctcct gggcgcgcac gagctgttgc gaaagccggc
     3661 gcagttcacc ttcagcgcgc tcggtaatcc gctgctgctc ttccgttctt cgctccagtc
     3721 gtgagactct caagatgctc aggacagcca ccagaatacc cagcaaaacc acgaaagcga
     3781 tcatccacgc cacatagcgg cggaacgccg cctggctgcg ccgaagcaac tgttgctggc
     3841 gctgaagatt gctcgcgttc agtttctgga tttcatgggc catgttcagt acggcgtcgc
     3901 gtcgcggcag cacctgccgc cggagaaaca gggagctgag ggcgatcttc tgttgtggtg
     3961 tccattcgaa aatcggatcc agggaatccc aatacgcgtc gatctcgcgc cgcaactcgc
     4021 ggagaacacc ggcatcctcc gcgcccagga ggcgctccag tttctccaat tgctccccca
     4081 tcgagttgcg gatctcacga agatgttcgc ggtgcgcctc ggcggtcaag tgcgacggat
     4141 cgagcaggaa atcgcgggca aggataccgg aaatgtggat ctgagagccg atcccggtga
     4201 gcgccgcggt gatctcccga tccgtcgcgt ggatattcat gatctcggac tggatctgcc
     4261 gtgcgcgccg cgaggcccca atccctgcga gagcgatcaa aaacacaaga ctgccgaaac
     4321 caaagatgag gattggccaa gttcgcgtcc gcatgacgtc gctctttccg cttactccca
     4381 ttctttcacg ccgtctccct tcctggaggt atggatcgca aagtctggac aaactagtct
     4441 ggagagtgat gatcgtcgtg tttaccgacc tcgacgggac ccttctcgac cacgagacct
     4501 actcgtggga accggcccgg cccgctctcg atgcgctcgc ccggcggcgc gttccgctta
     4561 tcctttgcac cagcaaacca cgcgccgaga tcgagccgct gcggtcggcc cttggcaacc
     4621 ggcacccgtt tatcgtcgag aacggcggcg cggcgttcct gccgcaggga tacttcccct
     4681 ttgccgttcc aggggcggcc tcccgcgacg ggtacgaggt catcgagttc ggcaaccggt
     4741 acgtggagct tgtcgaagcc cttcgcttgg cggcccatac ggccggggtg cccgtgcgcg
     4801 gattcagtgt gatgaccgcc gcggagatct ccgagcgttc cggcctctcg ccggagaagt
     4861 cccggttggc gaagctgcgc gagtatgacg agcctttcta tattctggat ccttcgcgcg
     4921 aacgcattct cctccgggag atcgaagtgc taggcaaacg ctggactcgc ggcgatcgct
     4981 tcttccacat cacgggatcg aatgacaaag cgaccgctgt gcgccggctc atggagcttt
     5041 accggcaagc tcacggaacg gtgaccacca tcggtctcgg agacgctccg aacgacgctg
     5101 agttcctcgc ggtcgtcgac aacccagtcc tcatccggtc ccggttctct gctgctcttc
     5161 aggctcttgt gccgaatggc cggcttaccg cccgtcccgg gccgtacggc tggaacgagg
     5221 ccattctaga gatggttcca gagtgaacgc tcgccgcgtt cctcttttgc ttccgactgc
     5281 tcctggaaat aacgtctggc ggaatcccgc ccgcctagac ccaacgccag actcgccgcg
     5341 aaaaccgctc cacccatcag gatgatgaat gctgccagaa acacgctccg ggcgaagttg
     5401 agctgatctg cggcgaccac aacggccacg aacatgatcg ccacccgaac cgcccccgcc
     5461 agtttcctgg gcgacggaac gccctcgttg accgcccaca caagcgtgct tcgccccaga
     5521 aactggctca accacacgcc cgccagcaga atcagtcccg cgaccacaag cttcggcaac
     5581 aggaagacga agctctgtgt tatctgcgtc gtaaggtcgg tgttgaacac gctcaggccg
     5641 gtcagaaaac cgcccagcag aatcagccag tacacgctct cggaaaccaa ccgggtggct
     5701 cggaggcgtc ccgaccggtc aagcataaag gccaggccgg tcctcctcag gaaccggtct
     5761 accgcaattc ccttaaagat ccgattcaac acccaccgcg ccagcaaggc cgcgatgtac
     5821 gcgccaagca gaatgatcag cgcggcaact aaggagggca gatacgtggt cgcttggtat
     5881 ttcagtcgcg ccagcacgcc ctccagaacc tgttgcatgg tttcgatcat ggttcaaacc
     5941 tccaggttcg cccgttcgac gacaccgctc ttcttgggga cgggcttctc cgccgccggt
     6001 gcgggctcgg cgtcccagtg agcgatcagg tacggtttca tccgctcgaa gcacaagcac
     6061 agctcttcga tcttttgctc gacctgcgcc gagaggagac tcgtggtttc gatcacgaaa
     6121 gaagcgaccc gcgcgaggta caacggcgtc aacgagcgta gcagatggcc ccggccgagc
     6181 ctcgctttcc ggtgcgcgca agcaaagtcc agtaccactc gcgtccaaag atcgtcgtcg
     6241 atatggaagc tggtggcgcc gggatgggct tgctcggcga gtttccgaat ttcgctgaac
     6301 gtgctcggct gcaacgcgat ggaccacacc tcacccagtt cctcgcatcc gtggcggaag
     6361 gcgttgagca tgcggtccag gtttacttcg atcggatcga gccccacgtc gaaacggaac
     6421 ccgaacaact ccacggcttc actccccgat cggttgcgcc aaaccggttc gtactcctgc
     6481 atcaaaagaa acacgctgcc cactacctgg tgcagcatcg cgctcaggtc cgcgccgggg
     6541 tccttggcgt cgtgcagctt cgccccaaga aaactctggc agacgcggaa tccttcggcg
     6601 agcgcgatgg ttgtcatcca gatgtcgatc ccgtagcgcg cgatgtcggt ctcccaatcc
     6661 tcacgcgaaa ggtaacgtga gagcagcctt gcggagacgc cgaattcgcc tccgatcggc
     6721 tgcctcactc ggtgtccata cagcgcgcga gtcagcggat acacgatgct gttcgtgatc
     6781 gtgccgtcat acttgtggcg gtgatagtag ggtgccacga agtcgtatcc tgcgaacaaa
     6841 accgggcgca acagcaggtc gaaccactct ggcgtgatcg aacggagatc cgaatcgacc
     6901 accgcgcacg ctctcgcttc cagccgctcc gccatctgga aaatcagacg gaaggcgcta
     6961 cctttgcccg gaatgccgtg gtacgggaac gacagccggt gtacaccggt caacggagta
     7021 gacaacagaa gaagattggc gtcctccatt cctgcggaga cgaccgcctg cgccgtcccg
     7081 tccttcgacc caccatcgga gttgacgatc accccggtca gatcgggaaa atatttcgcc
     7141 agccccgcgc aggcggcgcg tgtcacgtga ccgatcgtcc gggcattatt gtagctcgga
     7201 atccctatga cgacgtccgc cttccggatc ttttccgccg ccctaagtgc atcgagaggc
     7261 agttggtttt ccgccatcct tccagtccta tgctcctcgt cccaccacca gtttcatgtc
     7321 tttgtcgacg gcgtcccgaa gaagatcgaa gtactcggga atcgccgcca gcacccggtt
     7381 ccagttcggg atcagtggca ggccgagcgg atcttcgata tactccgccg cggcctggtt
     7441 caggctcttg gcgaatgccg cgaccgccat ctcttccccg tgccggtcga acttcaaacc
     7501 gttcagcatc gcgtcggcgt aatagcgcgc gatcgtgtcc tgcgccattc gcacgtatcg
     7561 cacgccaagg ctgcggaagt gatcggccgt gaaaacgatc ccttcggcgg ccatcgtgcg
     7621 aaacagcgac ttggcgatgt cggcggccat tcgccgcagc cccttcgtcg cgtcttcggc
     7681 ggatagcgtc tggtgcttgt gctcgtagtt gtccgtcaaa tccacctgac agatccgcgc
     7741 ggtggcgcag ttgcgataaa cctccgccag cacgcccacc tcaagtcccc agtcaccggg
     7801 gatccggttc acacgcgcca ggttggcatc cattgcgaac tccccggcca gcgggtagcg
     7861 aaagctgtcc agaaatcgca gaaagtccgt gccgggagcc atgctgtcca gcgcccgcac
     7921 cagcggagta acgaagagcc gggtcactcg tccgtgcatt cggtcggtca ctcgtgcgta
     7981 gtagcccttg cagaactcaa aaccgaggtt cggatttgtc accgggtagc aaagacgagc
     8041 cagtaactgg cggtcatagt tgacgatgtc gcagtcgtga agtgcgatca cgtcacagtc
     8101 gcccatcgcc agcatgtaac cgtacgccat ccagcaggaa cggcccttgc cgtcccctcc
     8161 cgctgagagc ccccgttcct caagtatctg gaacagcttc tggatccgtt cgctgtcgat
     8221 ccaaatcaag gtcacaggcg tatagaaatc ctcgaagtag gatcgtgcat ggtcgaactc
     8281 ctgccggctt gcacgcccga gggcgaccac gatccgttgt aggtacctga cctgcttcac
     8341 ctctggcaca atccgctgca tcgccggtgt ctgaaattcc gagtacagcg ccggcagcac
     8401 gagcccgatc ggacggttcc tggaaaagcg ctccaggtcc ccctccagcc gttcgatgct
     8461 gttcggccgt agccggtgta aagtcgtcac ggcaccggtc tggtagaaat cggacataac
     8521 ggtggcctct gtccgagggt agacggcgct ggcgcgcgga ggtatccccc ccagggagta
     8581 gtgtggagag gaaaaggggg tagcttcagc cgagccggag cggtgcctct tcaggaacaa
     8641 ccggggccag gttgcggtag gccagtctca agagaggcgg cgccgccagc gtggtcgcaa
     8701 ctgccataaa gacgaccacc ccatagacgt gttcgcccag caccccgagt cccaggccga
     8761 tctgggcgac caccattcca acctcgccgc gtgggaccat cccgacgccg acccggaacg
     8821 cgtccgcccg gcccaaccgg atcgcaccca tgccgcaacc caccagcttc gcgagtaccg
     8881 ccgccaccac gatcagcagc gcgagcgcta ggatcgaaga ttgcgagaac accgacaggt
     8941 ccacgtgcag gccgatcgaa gccaggaaga acggcacaag cagttccgtg acaccatgag
     9001 ccaggtcgtg gacgcggtgg ctgacgcttt ccgagagcgc catcccggcc agaaatgccc
     9061 cgatgatcgc cgcgaccccg gcgtagaccg cgagcagcga gagcccgaac agcagcacca
     9121 tcgcaaacgc gaactgcgcc tcgcagatgt gcaagcgttg ctgcacccgc ggaacgactc
     9181 gctccatggt ccgcgtaccc cacttggcga cgatgatagt gaatgcgatg gccactgctc
     9241 ctgtgagagc cagttcaagc agattaacgc ttccctttgc catactgctc accagggcca
     9301 ggacgagcag cccgagcacg tcgtcgatca ccgctgcggc caggacgatc cggcttgcct
     9361 tagcctgaag ccacccgccc gacgccagga cctgcgcggt gatccctacg ctggtcgcca
     9421 ccattgccgc gcccacgaag atcgactcca gttgcggctc gccccagaac tgaaagattc
     9481 cccagccgag cgcgaagggg acgatcacgc ccaggatcgc caccgccaag gcggtacccc
     9541 caacccgcat cagctcggac gacttcactt ccagcccaac gcggaacagc aagaacatca
     9601 caccaaggtc cgcgagcgcc gtgatcagct ccgcgggttc gatccagccg agtacgctcg
     9661 gcccgatcag gattcctgcg acgatttcgc ctacgatcgc cggctgccgg agccgttcga
     9721 aaagctcggc catgagcttc gccgagccaa agacaatcag catcgacagc gggatcttca
     9781 ccgcctcggg tccggtggca agcagcaagc tgaacgcggc ggacagatcg aagtgcgtca
     9841 tcaggtgcct ttttcgattc tataacaggg tctgcgaatc gtccgattgg cgagttccat
     9901 gagccagtac ggattccaac ttgcgccgca attccgccgc cagtctgggg ctgtggcctc
     9961 cggttgagac cgcgatctgc acttcgcccg aacggactcg cgccgggacc aggaaatcgc
    10021 actcttcggg gcagtcggcc acgttgaccg ggatacctct ccgcttggcc tccagtgcga
    10081 ccgcccggtt taccttccgg tcgttcgtgg ccgcaaatgc cagcgccatt ccgttcaggt
    10141 ccgagagtcg gaaccgccgc ttgcgcaggt gggcgccgag ccgctcaaac tccggcagcg
    10201 cctcgggtgc cacaaccgtc accctggcgc ccgtttccag cagtcctgtc accttgcgga
    10261 gagctacctt tcccgcgccg gcgacgagta ccggcctgcc cgacagggcg agaaaaatcg
    10321 ggtagtggag catcgagaag ccaatgctat catgaaggtc catggcggag tctccccgcc
    10381 tgcccacggt ggtgatcgtg ggccggccga atgtcggcaa atccactctt ttcaattgca
    10441 tactcgggcg gcggcgctcc atcgtcggcg acgaatcagg catcacgcgg gaccgcatcc
    10501 acggcgaggc cgtgcatcgc ggcaggcact ttgaactgat cgacaccggc ggtatcctgc
    10561 ccaacgaagc cgaactgatc cccagcgaga tcttccgcca ggcgcgcgtg gcccttgagc
    10621 gggccgcgca tatcatcttg ctgatcgacg ggcgcacgga gatcaccgcc accgaccgcg
    10681 agctggcgca actccttcac cgcctgggca aacctgtagc cctcgccgtg aacaagatcg
    10741 acactgctgc ccgggacgtg ctggcgcatg agttccacgt gctcggcatt tcagacctgt
    10801 tcccggtttc cgcggagcac cgcctcggta tcgacgatct gctcgatcac gtcaccacgg
    10861 gcttcgacct tactgagatt caggaagccg aaaaggcgcc gatcaaggtc gccatcatcg
    10921 gccggcccaa cgtcggtaag tcgactctgc ttaataccct cgttggcgag gatcgtgcca
    10981 tcgtctcgcc tgtcgctggc actactcgcg acgctgttga tgagagcgtc gtgatcgacg
    11041 gctgccagtt tgtcttcgtc gataccgccg gcatccggcg caagggtaag acgaagctga
    11101 tggcggagaa aatcagcgtt gtgatggcgc gccggcacat tcgcatggcc aacgtcgtgc
    11161 tgcttgtgct cgacgccacc gagggagtcg tcggcctgga tgcgaacatc gccggttatg
    11221 cgcacgagga cggccgcgcc atcatcctct gcgtcaacaa gtgggacgtc agcccggaca
    11281 agagcactaa gacgtttact acgaaagtgc gcgacgagat gaaatacctg gagtacgctc
    11341 cggttgcctt cgtttcagcg aagaccggcg cgggcgttca ccggcttttc ccgctgattc
    11401 gagagctata tgatgcggcc tcgcagcgcg tttcaacggg cgaactgaac cgcttcctcg
    11461 aaacacttca catcgagtcg gagcaaaagg tgcagtacat tacgcagccc tccgtccgac
    11521 ccccgacgtt cgtcctcttc acggaccgcc cgggcaagct gcacttctcc gccgagcggt
    11581 acctgatcaa ccggctgcgc aaacagttcg gtttccgcgg cacgccgatc gtcattaaga
    11641 caaaagcgaa gcagcggcgg tagataagag cgctcacttc acgcgaaaca tcgcgatgta
    11701 ctgcttctcc ggtgcgtggt cgcgcctcca tagcagttcg tagcccgcgg cctcgatctc
    11761 ctgtacaacc tgctccgcgc tggcccggat gtgcgtcagc gcgaaatcgg gatctctctc
    11821 catagcaccc cgcttcttgt ggtactcaac gattgccagg cgccctccgg gacgcaggct
    11881 ctgcttgata ccagccagca tccgttcggg gtagtcgaag tggtggtata cgtcgagcac
    11941 aatgacgagg tcggccgcac ttgccggcag cttcgggtcg gtttccgtac ccaacacgaa
    12001 ctccacgttg tgacgcgcgg cttccttgac gcgctcccgg gcgaggtcca ggaaatcctg
    12061 gtggatgtcc tccgcgatta cgcgcccttc taccccgact gccttgctga ggtctcgcaa
    12121 cagggttcct gtgccggtgc cgaggtcgac caccgtcgag ccggggcgaa tctcgagccg
    12181 ttgcatgagt tcagccggcc ggagattctc cagtcgcgag ggtgattcca ggacccccgc
    12241 catcctcgcg cgcccttctt ctgtctcgta ttcccggttg gcctctttgg cgacctggca
    12301 ccagccggtc gtcgaaagca gcagcaatcc cagtgtgcgt cgcattgaca cccccgttcg
    12361 ctatttgact gcgcccaaaa ctactccgat tcaagaaatc gagacgagct tcaactcttc
    12421 gtcggaagcg gcgtcacctt cggttgcagt cccgccttgg ccagcagcgc gtacaccagg
    12481 aagcccgcaa ggaagccgta cacagccgct ggttgcaggt acggcttggt ctcttcggcc
    12541 accggcagga acggcaggat gccgatcagg aacccgaccg cccaggcgcc gtagcccgcc
    12601 cagttgaccc cttctctcgg gcccgcccac ttgtttccgg agagcagata gtccgccgcc
    12661 attgcgccgc agatcggtcc gaacgacgcg ccgacaatcg taaacagtcc gatcaggttg
    12721 ctcgccgcgc ccgtgatcgc cagtacgagc gccaccgtca cgcccaccat cgtggacgcc
    12781 atgcgccgca cgccgggaat catcgtggcg aagctgtttc cggcgatgaa ggcgcagaag
    12841 cacgccggtg gcgccgatgc gatcgcgaac accaagaaca tcgccgcggc caggaaaccc
    12901 ccgatcccgg agatcaccgc gtcgtacgtg aagccctgaa caccgtacag cacccgagcg
    12961 cccgccaccg aaagcagcgg cagcccgccg gcgatcagaa tcgccagtgt gattccgacc
    13021 agcccgccca tcttcacgtc gttcgcgttc cggctgttca tgccgaagtc cgctcccgcc
    13081 gcgccggccg tcgcgaaaaa cccgacgacg atttggacca gcagcatgaa gccgacgtac
    13141 ggattcgggt cggcgggcac atgctgtccg atgccaaccg cggtccggaa gaacaccagg
    13201 atgatcaaca gcagcggaat caggttgaga tacaaggcga actttgcgac gtactggatg
    13261 cccttcaccc cgatgtaggc catcccgtag gcccacacga tcgccaccag cgcgaacggg
    13321 agcgtgcccg ggccggcctt cgatccgaat gcgctcagga tgaacgtcga cgaaaccagc
    13381 gtgttgaccg agaaccagcc gatttgcagc aggcccatca gcaggcccgg catgagatag
    13441 ccgcccgtgg ttccgaaagt cgaacttccg accacgtaca gcggatatcc ggtctgcatg
    13501 ccgagcatcg ccggcacgcg atagaacaat gcatagctga tcagcccggc aacaaccagc
    13561 gccagcaggc acaggcccag gccggcgcgg tcgatcgtcc ccttggcgat ttccatgtag
    13621 aaaaccaccc agaggaaaat gccggcatag ctcggcgcag tgttgatata ccacggcgca
    13681 cggtttgagg gagaattcgg tactgctttg gaaagatagt cgggaagcat gtccgctcct
    13741 caagcaatgg aatggaaaga gcggaccagt atactacctc cgggacgcgg caaaggcgtc
    13801 cgctcgccga ttcgtcaccc gccggagccg tcgtgctcgc cggtgccggc taactcctcg
    13861 accttggtgc cccggcagtc gagccgttgc ggaatcgagg cccgcgcgtt cacctgctct
    13921 tctgttgcgc ctacatcgcg atggcgcgag accgaaggtg cgtgcgcggc tgcagcggct
    13981 ggctgcgaca cttcattcat gatgtcttgc aaggtcgctt ccagcagcag gaacagcgtt
    14041 tcaaggtccg gccggctgag cgcgcggcca gcctcggaag gggagatcgc ggtaagagtc
    14101 accgatccgc cctcctcaaa cgccgccagc cggcagggca gcatcgcgga aaggcgcaag
    14161 tcggctccaa gcagagccgc gctcaggtcg gggtgacata aggagaacac gatggcgtct
    14221 ttcgcggcgc caggctcctt gttccggatc agatggccaa gatgcatgac cgacaccacg
    14281 ctggcgccgc gccgttgcgc tgcctgccgt aacgccgcct cgattccatc aagcttcagc
    14341 gatgacgtaa ctcgcagcat agaagtgcgc tcccgagaag atcatagcag aggctccttc
    14401 gctatcgtgc gcgctcgaca aacaaatcca caatctcgag cgccgtgctc cggatctggg
    14461 cctcgagccc ggagtacccc accagcgcgg cttgctctcg aagtctcgca gcggcctgcg
    14521 gcgaaacctc catccggtag caatcggcgt gctctcggtg ggaatactcc aggtagtgca
    14581 cagccgcgct ctccgcggcc tccatcagga catcccaata gctccttcgg cgtagcagcc
    14641 tcttcttcga agctacctct cgcggtagat cgatccggca accaggtccg cgcagcacca
    14701 cccgaaggca tcgctcggga gcgaatggca ggccgggctc ggagtccggg aaggtggcca
    14761 ggatccgttc gatgctctct cgatcggcct cccatccttg ccaccggaag cgtgtccctc
    14821 cgctcaccag ggtgccacgc agcagcagtt cgtggatacg gccgggatct ttgccggcga
    14881 ttcccagcat cagctcgatc aattcccgcg cggccatttc gcgcaccacc acagccgtga
    14941 tcgagatcgc ccccgcttcc tcggagctga gtttgacccg aatcgactcc ggtagcgcca
    15001 tgcgcaacaa gtgtagaaca tcgtgccggg cgtccgccgg gggagcccca acaccccccc
    15061 acatttttca acctttctcc ccactttgaa ccaaaccatg cgttataatt aactgcgacg
    15121 cttgactctt gtattcggtt tgccaaaggt tcccaagaag gtccacacga cccgccaccg
    15181 gagtagtctc gactaatgcg gattgcctgt agcggaaatg ctgtgtcctt ttttgccttt
    15241 gtgcgccggc tcgggacaag cgagttgaca cgggaaggta aggaaggtcc atgaaggaaa
    15301 aaggtacagt gaagtggttt aacgccgcca aggggtacgg attcattcag cgcgaaaacg
    15361 gggaggatgt cttcgtacat tactccgcca ttcagacgga tggctaccgg accctcgagg
    15421 agggttccaa ggttgagttc gaggtcacgc aaggtccgaa aggattggcg gcggccaacg
    15481 tcacaatgga cgcctagtgt ggctttttgt tcgcggccgg ccgggagccg ttcggagccc
    15541 ggctcgccgc gatccgcggt ccgtcaagag ccgcgctgtt gcatagaagt gagctgaaat
    15601 ccagcaagga ggatgatgag taaagaggac tgcatcgaag taaccggagt ggtagttgag
    15661 aagtttccca gcggcctatt tagcgtgcag cttgaccagg accgtgtgat cctggcgcat
    15721 ctggcaggca agctccgccg aaaccgcatt cgggttctgg cgggtgaccg cgttacgttg
    15781 gagctttccc cctacgatct gaccaagggc agaatcacct atcgccacaa gtaaccgccc
    15841 cgcatcccca cggaacatcc tgttcgtatt cggcactcgg cccgaagcaa ttaaactctg
    15901 tccgctagtc gtgtacctgc tcgcgtccag gcgtttcacc gttcgtgtgt gcgtcacggc
    15961 gcaacacagg cagttgctgg accaggtgct tcaggcgttc ggtgtacgcc ccgattacga
    16021 tctgggcgcc atgctcccgg accagacgct tttccaatcc acgtcccgga ttctctccgc
    16081 gctcgagccg gtgctaggaa ccgagcggcc ggatctcgtt ctcgtacaag gggatacaac
    16141 caccacccta tgcggagccc tcgccgcctt ctacgcgagg gcggccgttg gccacgtcga
    16201 ggcgggactg cggacacacg acaccgcgcg cccgtttccc gaggaaatca atcgcgtcct
    16261 cgtcggccgg ctggccacac tccacttcgc agccactgag ggcgccgccg ccaacctccg
    16321 cgccgagggg gtcgcccccg aacggatctc ggtcacgggc aataccggca tcgatgcagt
    16381 cctctatgtt cgcgatgcgc tcgagcaggg ccggctccac ggcgcggggc tgcctccgct
    16441 tgacccgtcc cgccggctgc tcgtcgttac cgctcatcgc cgcgagagtt tcggcccggg
    16501 atttgaaaac atctgctcgg ctctcgaacg cttggcccgc cggcccgacg tccagatcgt
    16561 ctaccccgtc caccccaacc ccaatgtccg tgagccggtc ttccggcgcc tctccgagtt
    16621 cgccaacgtt ctgctcaccg aaccgctcga ttacgtcacc tttgtcgact tgatgcggcg
    16681 gtcctcgatc ctgttgaccg attcgggtgg catccaggag gaggggccat ccttcgggaa
    16741 accgatcctg gtcatgaggg acaaaaccga gcgcccggaa gccgtcgagg ccgggacggt
    16801 ccggctggtg ggcgccgacg agaaccgcat cgtggagcag gcgtcccggc tcctcgacag
    16861 cccggacgaa taccaggcca tgtctcgcgt tcacaatcct tacggcgatg gccgggcatg
    16921 cgagcgaatc accgccgtaa tcgactcatt cttcgataga tagaggtttt cctggttact
    16981 ccgaaatagt ccgttccgcg cgtgtttccc cccggttttt tgcggccctt ccgttatact
    17041 gcggccggga ggggacaccg cgcatgaaga acggcgccgg aacgcgaagg gcaaagagga
    17101 acctcgtgca gggctcgtcc ggcgtggcgg aaatgctcgc ctccagagtg gttggccagt
    17161 ctcccgcgct cgacgccatc gtgccctgcg tcgagatgta ccaggccggc ctctcccccg
    17221 aaggacggcc cgccggggtg tttctgctgc tcggtccgac cggtaccggg aagacgcaca
    17281 ccgtggaagc gctggccgac gtccttcatg gaagcgaaaa gtgcttgctc cgaatcgact
    17341 gcggcgagtt ccagttggag cacgaagtgg ccaaactcat cggcgctccg cccggctacc
    17401 tcggccaccg cgagacgcag ccgctgctga cccagcagaa gctcaacatg gccgcttccg
    17461 aggggtgcgg cctgtcgctg gtgctgttcg atgaaatcga aaaagccgct ccgtcgctga
    17521 cgcgcctgct gctcggcatt ctcgaccggg gcacgttgcg gctcggggac aatgccgtcg
    17581 tcaatttcga gcgaagcctc atcttcctta ccagcaatct gggagcgcgc gggatgatgc
    17641 gggagctccg tcccgttttc ggcttcgagg ccggcgtccc caaagagcgt gcctccgtgg
    17701 accgaaaact cgcaaacatc ggcagggaag cggtgaaacg caagttctcg cccgagtttg
    17761 tgaaccgtat tgaccacgtg atcacctacc ggccgctcga cggcgactcg ctgtcgcgca
    17821 tccttgaccg gcacatcagc gacttccagc ggcacgtcaa ctcgcgcctc ggtcccgatg
    17881 gcttcggcat tgaagtaccc tccgagaccc gccgtttcct gctgcgaaaa gggaccagcg
    17941 ccgaatacgg cgcccgggaa ctgaagcgaa ccctacaccg gcatctcacg caaccgctgg
    18001 cggcgctggt cgctggaggc cggatcgagc cgggctcgag tgtgagaatt gaaatcgacg
    18061 aggacgagga tgacgtgttc atccgatact aagggggtgg ctcagcccgg cacgctgtat
    18121 cttgtcgcca ccccgatcgg aaacctcgag gacatcacct atcgcgcggt gcgggtcctt
    18181 cgggaggtgg acgtcatcgc ctgcgaggac acccggcaca cgcgcaagct cctggaccgt
    18241 tacggaatct ccacgcctac ggtgagctac cacgagcaca acgaggcgga gcgggccgaa
    18301 gcgctgttag agcggctgct tcacggaact tcggtggcgc tggtttccga cgccggcacg
    18361 ccgctggtct ccgaccccgg ctaccgcctt gtgaacggag ccatcgagca cggcgttccg
    18421 gtggagccct tgccgggtcc ctcggccgtt cttgccgcgc tcgccgcctc cggcctcccg
    18481 acggacgcgt ttcactttgg cggcttcctg ccctcgaaat ccacccagag gcgcaaggtt
    18541 ctggagacgt tgaaggacga acaggcgact ctggcgttct atgaagcccc gcaccggatc
    18601 atcgagtcgc tcgccgacat cgaagcggtg ctcgggccgc ggcgtgttgt gatcgcgcgt
    18661 gaaatcacga aggttcatca ggagttcctg cgaggcctcc cggctgagct cagaggcatc
    18721 ctcgaagcgt ggcccgcaat caaaggtgag attacagtgc tcatcgccaa gggaacctcg
    18781 cccgaagccg gggacgagac cccactgccc gaggcggttg cggaactcgt cggaagcggc
    18841 ataccccgga tggatgccat caagaccgtc gcccgccgcc gcggtctctc caagcgggaa
    18901 gtctacgacg ccgtcgaaaa ctcgcgaaaa cacggctaac ccccgctcgg tgaattgcat
    18961 gacccgagtg tccgttttcg ggaatggctt tgtttggtca acgcggcgct gatgtgaccg
    19021 aagtcatcga cgtacaataa gtgaacctgg gatgctgaag cttacccgaa atctcggagg
    19081 atcttcattt tgagaaagct ctggattact tttgccattg tcgtcgtcgc gtcctttgcc
    19141 gtcctcggct ggatcgggac gcgcatcttc caggaaatgc cgccgatccc ggaccgtgtc
    19201 gtcaccacgg acggacagga ggtcttcgcc ggcggcgaga tcggtcgcgg tcaaaatgtc
    19261 tggcagtcgc tgggtggtat ggaagtgggc tccatttggg gccacggcag ttacgtcgct
    19321 ccggactgga ccgcggactg gttgcaccgt gaggcgatgt tcatcctcga cgagtggggc
    19381 cgcaaggaat tcggcaaatc atacgaccaa ttgccacccg aaagcgcagc gcagctccag
    19441 ggccgcctcg agcagatgta ccggaccaac acctacgatc cggcgacgaa aacactcacg
    19501 atcgaccctg tccgggcccg cgcctttgaa gcctcgctgg tgcactactc agatgtattc
    19561 atgaatggca acaccgccta ctcgattccc ccgggcacag tacgcgacgc cggccgcatg
    19621 cgccagttcg catccttcat gttttggacg gcgtgggccg cgtctgccaa ccgtcccggc
    19681 gaccaggttt cctacacgca taactggcca cacgagccgc ttgtcggcaa caaacccacc
    19741 ggagaaagcg tgatgtggac cggcgtgagc atcatcatgc tgcttgccgg catctccggg
    19801 ctcgtctggt ggtacgcggc gcaggcgcat gaggagcaac acggcccgct cccaccggtt
    19861 gaccccctcg gcgcctggag ggcgagcgcg tcgcagttcg ccactctcaa gtacttctgg
    19921 gtggttgccg ttctgattct ggtgcagatg ctgatgggcg tcgtcaccgc acactacggc
    19981 gtcgaagggg acggattcta cgggttcccg ctctccaagt ggctgcctta cagcgtttcg
    20041 cggacgtggc atgtgcagtt gggcatcttc tggattgcca ctgcgtggct cgccgccgga
    20101 ctgtttatcg gcccgctggt cagcgggact gagcccaagg gccagaaact gggcgtgaac
    20161 gttctattcc tggcgctcct cgtcgtcgtc gccggctcgc tcaccgggga gtggctcagc
    20221 gtgcacaaca agctctcgga cgccacatcg ttcctgtggg gccatcaggg ctatgaatac
    20281 ttggacctcg gacgaggctg gcaggcgctg ttgttcgtgg gcctgctgat ctggctgttc
    20341 ctggtcgttc gctccattcg cccggcgctg aagcagcccg gggagcagcg gccgttgctc
    20401 tggatcttcc tgatgtcggc ggcggccatc ggtttgttct atggggcggg tttgaactgg
    20461 gggcagcaca cgcacctgtc aatggtcgag tattggcgct ggtgggttgt gcacctctgg
    20521 gtggagggat tcttcgaagt gttcgcgact accgtgatcg cattcttctt cacccggctc
    20581 cacctcatcc ggcctgccct ggccgcgaaa gccgccctgc tctcggcgac catctatctt
    20641 gcgggaggca tcatcggcac ctgccatcac ctgtacttct cgggcactcc gaccgtggcc
    20701 ctggcatggg gctccgtgtt cagtgctctc gaagtagtcc cgctaacgct ggtggcttac
    20761 accgccgtgg atgacctgcg ccgcggccgc ctcacaccct gggcgcaacg ctacaagtgg
    20821 ccggtctact tcttcgtcgc cgtggcgttc tggaacatgg tcggcgcggg gctgttcggc
    20881 ttcatgatca acccgccgat cgccctctac ttcatgcagg gcctgaacac cactccggtg
    20941 catggacatg cggcgctgtt cggtgtgtac gggatgctcg gcatcggcct gatgctcgta
    21001 tgcctccggg cgctgctgcc ggacctccag tggaaggaca agtggctgag gttcgccttc
    21061 tgggcgatga acatcggcct gctcacgatg tgcgtgggta gcttgctgcc cgtcggcttg
    21121 cttcagacct gggcgtccgt ggatcagggc tactggtacg ctcgcagcaa ggagttccta
    21181 ggcacgccgc tgatgcagac gctgcgctgg ctccgcgtgc cgggcgacac gatcttcgcc
    21241 ctcggcgagt tcgcgatcgt cgctttcgtg ttgaccatcc ggccgggcaa gtcgcgagcg
    21301 tagttcacac gcccgccgtg ccgcatcgtc aggacgacac ggcgggcttc gcgatgtcca
    21361 cccaacgcac gccgagtttc cgggcggcgt agcccaccgc ggccaggtga tcgccgtacg
    21421 ccatcaccca gtgggagtcg cgcgcctcgc ctctcagcag ttcccagttg ccctggatct
    21481 cgacgtcctg ctgggagcgg cagatctcat agaacggatt ggcccgcaca atgcctttga
    21541 agcccaccca gcaccctgaa ctgtactccg ggtcgataaa ggtgacttcc tgcccgatcg
    21601 ggatttccac tttcggcgcg gcgccgtact cggactcgta atgtgtggtg atgagcgccg
    21661 ggttgtaatc cttgccgttt atccggcgcg ggccggtgca gtgagcgcag gtgacgattc
    21721 gctgatgggc gatctggtag tctcagcatg atgcgggtgg ccatcttcgg aacgattctc
    21781 gcggccgtgc ctatcctcgc gcagatgccg cagaatccgt cgccgatggt cgaacacacg
    21841 cgagcgcacc gtcgcttgac gcaggagacg cctccgggac ggcgggaagc gatcgagccc
    21901 ggtacgctct tcctgccgga tcggctgcgt ctcaagaacg gcatcccgct gctcgtcttc
    21961 tttcacggcg gcgcgtggat ccctgaggtc gccgcggcgc ggcatcggat ggcggtgatc
    22021 tccgttcaga tcggtgcggg ttctggagtg tatgcccgtg ccttcgccga tcccgcgggg
    22081 tttccggccc tgctggcgga agcggagcgg agggcgggcg ttcggatcgg acgcgtgatg
    22141 ctcggaggat ggagcgcggg gtgcggcgcg atccgagaga tcctcaagtc cccagagtcg
    22201 tatgcccgtg tcgatgctac tctgatgatc gacggcatcc attccggtta tgtcaacgga
    22261 aagcctggcc cgctcgaatc cgaaatcgag accgatgcgc ttcagatctg gctgcagctg
    22321 gctcgggatg cgatcgcggg gcggaagcgc gcgatcatca ctcattcgga gatctttcca
    22381 ggcacttttg ccagcaccac ggagacggcg gatttcatca tccggcagct tggcttcaag
    22441 cgccgcgcgg ttctgaagtg ggggccgatg ggaactcagc agctcagcga agtccgtgcg
    22501 ggccgcttcc tgctgatcgg ttacgccggt aacacagccc ccgatcacgt cgaccaactc
    22561 cacgcgctcc cagagtacct gaaatggctc aagtaaaccc cgaccgccgg cggcatctcg
    22621 ttctattggc cattgtggcc gctgccttgg tgtggtccgc aatccgcccg cgtgactact
    22681 tcacatggtt tctcgaggtg gcgccagtct ttgtgggctt tgtgttgatt gctgcgacgt
    22741 accgccgctt tccgctcaca acattcctct actcgctgct cgctgctcac tcgttgattc
    22801 tgatggtcgg cggacactac acttacgcgg aagtgccgct gttcaaccgg ctgcgcgaca
    22861 tcggcatttt ctcccgcaac aactacgaca aggtcggcca tttggcgcag ggctttttcc
    22921 ccgtaatcct ggctcgcgag atcctgatcc gcacctcgcc gcttgccggg agccgctggc
    22981 tcggtttcct gagcgcttcc gtgtgcctcg ccttcagcgc gctctacgag atcatggaat
    23041 ggtgggtgtc cgttctgggt ggaagtaggg gtgacgcgtt cctcggcacg cagggttaca
    23101 tctgggacac acagtccgac atgttcctct gcctggtcgg ctcgatcacc gctcttctgc
    23161 tcctggccca ggcgcacgat cgcgcactgc gccggcttcc gggctagcgt cgggagtcgt
    23221 tgcgcggctc catcatccag acatttcctc gaagttccgc catcgggaag aacaactggt
    23281 tgtttcccag cgtgagagtc atctccgaca aactgatcgc cgaaagggag tgctgaatcc
    23341 ggtgaaaatg gccgactggg aacacttccc cgagcggctg cttagtcaca ggattcaagc
    23401 gcctcgccca gatgcagcga tagccgtccc gatccgacgt gaagtagatc agcctgttgt
    23461 tgggtgccca aagaggcaga tcgtcccatg cgccgccttc ggtgacagcg atccattcgt
    23521 tcactggcga cgccgaatct tccttcaccc gtgcgatgaa gatcctcgta cggtgcatgt
    23581 ccagatcgac cttgaacgag acccactgcc cgtcgggaga gaagcgcgag acgtacaccg
    23641 gaagctcggg gtgcgcgagc aacagactct gacgtccggt gccaagatcc aacaggtgga
    23701 tggtagcgcg cggtacgtcc gggcggaggt aggtaatccg ttttccgtcc ggcgaccagg
    23761 tatcgggcgg gccgcaatcc aggcagactt tcgttgcgag cccgctatct atgctcaaag
    23821 tgtagacaga gcgcttgttg tcttcgatca cctgaaaggc caccgtcttc ccgtctggtg
    23881 cgatcttcgg catgctcgag aagactctag gggcatcggt cagggcccgc tccgctccgg
    23941 tgatgagatc ctttgtccag atctcgggtg atcgcgtgcg gtccgaccgg aagacgagcc
    24001 gccgcgcgtc cggtgaaacg tcacacgcgc tatcattgcc gccggcgtca gtgacctgtt
    24061 ttggctcctc ggatgcctcg ccccggttcg cgtcgacttt cacgctccac aacgtcagcc
    24121 tgcgcgaggc gctcatgaag acgatccgcc cgcccggccc ggccgaagca tctccctccc
    24181 cgacgccgaa ggtcagccgc tccacgggcc ccttggcttt ccatgtcttt gggtcaatcc
    24241 agatccggca caagttgctg ctgtctccag accggcctga aaataacagg ctgccgctgt
    24301 ctccggactg gcccgggaac acgaggtctt ctgtgttagc gaggtaaccg tccagggtcg
    24361 caagacccat tctctggttt ttcagaattt ctacgactcc tgtacgcacc acagggcgtc
    24421 catccagcgg agcgacccag acatcgaatc catacgttgg atgcactccg atgaacatga
    24481 ggtgtttacc atctcgcgtc cacattggct ttgcagccgc agggaaatcc ggcacgagtt
    24541 gtctcgcctg tccgccgttg actgatgcga cgtacatctg cgtgccaaga agtgcgccct
    24601 ttataccgac gaaatacgcg atctggctgc cgtcgggcga aatctcgggg gcgagtccct
    24661 gtttcacgac cagccgtgaa tccccaccca gcgtgggaac cacataaatg ccgccaccgt
    24721 cgcgttctga gcggaagaag attctggagc tgtcggccga gaaagccggg ctgtggtccg
    24781 cggtttcgtg gtccgtcaga cggatcgttg ccccggagtc aagttgctga acccagatgt
    24841 cgaggttacc cccgccggca cggtcggacg cgtaggcgac cagtttgccg tccggtgaaa
    24901 aggccgggta gcccgtaaac cccgagtcgc tggtcagact cttcatccgg tattggctct
    24961 gtggagtcga cgccggtccg gtttctcgag taaaccacca caccaccgag agcgcgacgc
    25021 agacgagcaa ccctgccgct acggcaactc ccgtgacgct tctccaccag ggggtggacg
    25081 gcggcaccgc atcactttcc agcgcggccg cactctcggg gccctttacc tccgcaaccg
    25141 gtgctatgaa ccggtagcct tttcggggta gcgtctcgac aaagcgcggg ttgtctgccg
    25201 aatcgccgag cgcccggcgc actttcttga tggccgtgtt gacgccgtgc tcgaactcga
    25261 tgaaagtgcc ctctggccag agcgccttct gcaaagcctc gcgggtgata accgctccgg
    25321 ggtttcgtag cagcagaacc agaacctgta acggttgctc ctggagccgg atcttccgcc
    25381 cgcgcttgcg aagctcacca gcggcgagat ccaactcgaa gagcccgaaa cggagccgag
    25441 ctccgccggc cgagccgggt tccatggtag ccgtccagag tggattgtag cacctgtaat
    25501 cacggtgtca acggtccagg cagatacata accctcattc gttcaatcgc ttacatgttc
    25561 agagccggcg ccgccgtcaa ggcgcgaatt acacattgag tccagccgct gtgcattgag
    25621 ggccatcccg gttcagccgc acgctgatcc cgcgatggag gcggaaccga tgaaggtcct
    25681 cgttgctgga attctcctgg cgggagttgc cctgcccctc catgggaata aaggggagga
    25741 gcctctcagg ctcagggtgt tcatgtacaa ctacgccgag acatcccaat cgcttgtgga
    25801 tcgggcccgt cgcgagacaa gcatgatctt ccggcgagcc ggggtagaga ttgagtgggt
    25861 gcaatgcacc ctctccatgc ggaatgctcc tcagcagctt tgctcgatgc gtcgcggtcc
    25921 agcgaacctc gaactccggc tgctgccacg acgcatggcg ggtcgcatga ggctcggatc
    25981 gaacgcagcc ggcttcgctc tgcaacctct ggacggcgtg cccggcgtcc ttgccaacgt
    26041 gttcccggac cacgcggcgg atctggcaac gaacagcggg ctcgacagag gtgtgatgtt
    26101 gggccatctg atcgcccacg agttgggcca cttgctgctc gggatcggga gccactcaac
    26161 cggcggaatg atggccgcgc cgtggggcag gcgggaactc tcatgggcga tgaagggctg
    26221 tctgacgttc tcgaggccgg aagctgagaa aatccgcagt cgccttcttc tacgtagcgc
    26281 cggcatgggg acaccgaacg ctgccatccg gtgatctgat accttgaacc cgatgggcgt
    26341 tagatcaacc gtctcgctgg atgcggcagc atggcggagg tagccgcgcg aacgcgggaa
    26401 ccggtgatcg ggccggtgct ccggactcta cggccgatca tctacctcac tgcattcctc
    26461 tggcggcgct tactctttcg aaccaccttc atcggcgtga ccggaagtct cggcaagaca
    26521 accgctaagg aatgcctcgg aacgatcctg gcttcgcagt atctcaccta ccgcacccgg
    26581 ctgaaccaaa acgctccact cggagtcgcg ctgaacatcc tccacgtccg gccatggcat
    26641 cggttcgcgg tgatcgaggc agctacggga caacccggca tgatgcggtc ttcggcacgg
    26701 atgttgcgcc cggacgtcgt ggtggttctg aacctcaagc gcacgcacac gacggcgttt
    26761 cgcactctgg cggagcacgc ggctgaaaaa gagcgcctgg tgcgcgccgt tcgccccggc
    26821 ggactcgcgg tcctgaatgc cgacgacacg cgcgtcgcgt ccctggccga tggcaagaag
    26881 cttcgtgtga ccacgttcgg cacgaacgaa gggtgcgacg tggtcgtagc gagggtcgcg
    26941 ggaacgtggc cggaacgctt gagcttcgaa gtccgcagtg gcttggagac cctggcggtc
    27001 cggacccggc ttgtcggcgt acattgggtc aactcagctc tggcggcgct tgcgaccgcg
    27061 catgcgctcg gatttcgcat ggctgacggc gttgaggccc ttcatgccgt ggagccattt
    27121 ccgggaaggt gccagccggc acgggttccg agtggggcga tttttctgcg cgacgactac
    27181 aactcctcga ttgactcgat cagtccgagc ctgagcgtgc ttcgcgacgc cgaggcggaa
    27241 cgccgggttc tcgtgatgac ggatgtgtcc gatctcggcg taaaccgtcg gggcagactc
    27301 aggcatctcg cacccgagat ctgcgcctcc gcggacagcg ccgtcatcat tggcgaagca
    27361 gccgagtttg ggaagcgacg cctggtggag gcaggtatgg cgccggagaa cgttcatgcg
    27421 tttgatgatc ttcagccggc cgcggagttc ctacgttccg aactgcgcga tggggacctg
    27481 gtgcttctca aggggcgcac cacggatcac gtggcacggg ttttcttcgc acaacttggt
    27541 ggggtgcgct gctggaagcc ctactgccgg aagaccatcc tgtgcgacca ttgcccggat
    27601 ctcggcactc ccccggaaca gcaaaggcgg gcctcgctgg tgagcctgcc gcagcggacg
    27661 ggcgaggtta cacgactgcc gtagcgccct gcaacagacc cttgatgtct ttgattccca
    27721 cgccggggcc cgtgggcacg ctcatctttc cgtccttcac agtgatcggg ctatcgaacc
    27781 aagccccgta tttctcagta cccattttgt actcctggta ccgccctggg tcacggacac
    27841 acgaggcgaa gtggagcatg tagacaaagc cgaacccgcc tgagatgtgt acggtggtcg
    27901 gcatgtttgc cacgtcggcc atgtgcgcga cccgtatgga gcggatcatg ccaccgtaat
    27961 agtgcagatc cggttgcacg atgtcgacgc cccggttggc gatcatccag cggaagcgcc
    28021 actcactgta ctcctgttcg cctccggcga cgggaattgt cagcgcgtcg gctactttct
    28081 tcgtgtcctc gagatgatcg aacgggcacg gttcctcgta gtagacagcc ttgatctcct
    28141 cgagcatctg ccccatttcg atggccttcg ggggatcgta ggagctgttg gaatccgcgt
    28201 gaatgtcgat cgcgtcgccg aacgtcttgc ggacgagcgg gatcagcgtc tcggtgcgcc
    28261 ccg
//
LOCUS       DATIFQ010000520         8757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_754634, whole genome shotgun sequence.
ACCESSION   DATIFQ010000520 DATIFQ010000000
VERSION     DATIFQ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754634"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..831)
                     /locus_tag="VLE22_26285"
     CDS             complement(55..831)
                     /locus_tag="VLE22_26285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB17987.1"
                     /translation="MPGTKTIRPYHWLALYYDRVFSFARSWGEAPRTHVLGRLLRRVV
                     SACDLACGTGSTAVTLARRGIKTFGVDLSPTMCRLAREKARRARVPLQVLHADMRAFR
                     LPEPVDLVLCEFDALNHVPRRTDLAKVTKTVARALRPGGHFYFDVNNRAAFKGVWPLT
                     LYIEKPGVAVVMHGGCDRSGERAWSDVEWFIREGGYWRRRHEHVEEVCWTASEIRNAL
                     RDAGFDTIRAWDAAPLLRNDPSIRPGYRTFYLARKQKTRD"
     gene            979..1476
                     /locus_tag="VLE22_26290"
     CDS             979..1476
                     /locus_tag="VLE22_26290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSB17988.1"
                     /translation="MTTAELQSVFEGNRDAVFGFAWRMTGSPETAEDIAQDCFLQLLK
                     SPGHYDGMRGPIRPWLLGVARNLILKRWREEGRWTALDEEELAVVPTSNDCRVDEKVA
                     HAVQSLPPLQREVLILIEYEGLTLEEAGRAVGAEVGAVKARLHRARNNLRRVLAPLRS
                     VNHDG"
     gene            1466..1744
                     /locus_tag="VLE22_26295"
     CDS             1466..1744
                     /locus_tag="VLE22_26295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17989.1"
                     /translation="MTDDELRSMLRTWQAPPAPETLRKRVFRRRWSPFRWLVQGEIRV
                     PVPLAVAVLCMLIFAATRVFRPSATSLSDFEQVQQFQPRIVRMVHETR"
     gene            1731..2591
                     /locus_tag="VLE22_26300"
     CDS             1731..2591
                     /locus_tag="VLE22_26300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17990.1"
                     /translation="MKLVSLILLTLSAYGAQLMSGGASSNGVWVSYETRLEPDSPSIR
                     KHGGGTLTEKNVIKRHLCNFDNNTYFGYDLVAEPLGGGRYRLRFAPLTITPRKMSEIF
                     SEANVSNWTPLPLPREPATLEIQAGETVALDLFVNPSTGQKVTDYLTIKGDERQVVHV
                     AGPARDFGPEDATIEISFPNVSVDGTPLVSSQGGVSGQAVWIDLPGHGRFVFSLAPHP
                     DLGMQKAGEIRGTTMTWRTGGHEYTITTDKPIASGSRAYNLYVFHIPRTANYFGMSAG
                     AKPDDPIRSR"
     gene            complement(2769..3482)
                     /locus_tag="VLE22_26305"
     CDS             complement(2769..3482)
                     /locus_tag="VLE22_26305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17991.1"
                     /translation="MKIEILLNNFAIRCFRDVGDGDYISARMAYRAGLTLQYLWASQQ
                     VIEKYLKCILLLNRIPAVDIKHDLEAALTKIANAGKIALDLTSPTKKFIDYIDTYGRF
                     RYLETSPYSFGSDIVKLDRTVWELRRFCTLDEGPRQIRLSDGLPAPIIRIEGGRLEKI
                     VDAPKDPAREPLLWQNGFFGKRTRRWLRVRGGFTATNSPLFLNPHLLDEVVKYVFLPK
                     EIKTAYRAAAKEKLYDGEN"
     gene            complement(3582..4904)
                     /locus_tag="VLE22_26310"
     CDS             complement(3582..4904)
                     /locus_tag="VLE22_26310"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB17992.1"
                     /translation="MENGGVSRRDFLRGSSVAAVQFPFVSKGLAADEVIRVGVIGCGG
                     RGTGAALNVVQAKTDVIYPPPGRGYHTENAVPGARAKAQNVQVVALADLFTNRLAECR
                     QQLKTVGVEISDRNCFTGFDAYEKVIGMPEVDYVILATPPQFRPREMRAAVEAGKHIF
                     AEKPIAVDAPGVRSVLESGRLAEKKGLGVVAGTVRRHSLDQVETVRRLRDGAIGKILE
                     ARAYFNVGEIWAIPREGGWSDLEWQHRNWPYYTWLSGDIIVEQHIHTLDLINWVLGEH
                     PVRAYGVGGRASHEPGGDFGHTFDHFAVEYEYPGGARLFSQNRQIANCDVRIGAAVVG
                     ENGTSNCENQIVADRGWKYQGEVPEAYEQEHVDLIESIRRGKPLNETRNVAEATLTAI
                     LGREAAYSGKVIEWDAALNSKRDYTPASYELGPAKMPDVVNPKEYRFY"
     gene            complement(4927..5613)
                     /locus_tag="VLE22_26315"
     CDS             complement(4927..5613)
                     /locus_tag="VLE22_26315"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB17993.1"
                     /translation="MLRAAVIIPALNEEESLGATLDRVPRSLFQTVIVADNGSADRTA
                     EVAREHGAIVVSEPERGYGAACLAALAAVPPDTEAVAFMQADSSEDPEEAALLLAPIA
                     EGRADLVLGSRVMGRAERGSVRIHQAFGNRLATFLIRILFGYRYADLGPFRAIRLESL
                     RALGMRDRNYGWTVEMQIKALQQGLRVLEVPVSYRRRLAGEEKVSGNPIASVKAGAKI
                     LWTVAKLKVW"
     gene            5656..6225
                     /gene="lepB"
                     /locus_tag="VLE22_26320"
     CDS             5656..6225
                     /gene="lepB"
                     /locus_tag="VLE22_26320"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSB17994.1"
                     /translation="MTGDNLPRETDGHRHHAPLWHVFAWARDLVMSLVIAAVVILFIY
                     QPVQVEGTSMEPALVNEERIFINKFTYRFGIEKIGRGDMVVFRFPNDPSKSFIKRVIG
                     LPGDYVLIENGRVFVNDRLLEEPYLPESFRDKASWGPSRIAQDYYFVLGDHRESSNDS
                     RNWGAVHRDLIYGKAVFVYWPLSKLGPVR"
     gene            complement(6274..7197)
                     /gene="msrP"
                     /locus_tag="VLE22_26325"
     CDS             complement(6274..7197)
                     /gene="msrP"
                     /locus_tag="VLE22_26325"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HSB17995.1"
                     /translation="MLIRIPRDWEIPESQATPEHIYLNRRQLLLAAGFLGVDRLVSAS
                     AGPYPAKRNPEFTLDRPVTEEWAATGFNNFYEFDPTDKQAVKNKVGSFVVSPWKIEVT
                     GLVNKPRTLDLDDLLRTMPFEERLYRHRCVEAWSMAVPWTGFPFSELIKAVEPKPEAK
                     YVRFVTVNRPKEMPGMVGAPWYPWPYFEALRMDEAMNALTMMVTGLYGKPLPKQNGAP
                     VRIVTPWKYGYKSIKAIVRIEFVSKQPATFWNKLQPLEYGFYSNINPGKPHPRWSQAF
                     EKVIPRMERRPTLLYNGYEKYVAGLYNGKES"
     gene            7256..>8757
                     /locus_tag="VLE22_26330"
     CDS             7256..>8757
                     /locus_tag="VLE22_26330"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSB17996.1"
                     /translation="MHEKTAIIVGAGPAGLTAAYELLSRTAIRPVVLEKSEFMGGIAR
                     TVNYKGYRMDIGGHRFFSKSDRVMNWWLDVMPMQAVPGAPPPITYQRQTRTVLGSGGG
                     PDPDREDRVMLLRKRRSRIYFLRRFFDYPIRLSAETLAKLGLVRTVRIGLSYLKSAAF
                     PIRDERTLEQFFINRFGHELYLTFFKSYTEKVWGVPCDRISAEWGSQRVKGLSIARSV
                     QHFLKQALRRHSDVAQKDTETSLIEQFLYPKLGPGQMWEEVARQVCGKGGEVLTGWDA
                     VRVRTDGFRVTGIEAVHASGERRLFEGDYFFSTMPVQELVRALDRPAPPDVVEVSEGL
                     MYRDFITVGLLVGGLKVKEDHPDGSKLIRDNWIYIQEPDVLVGRLQIFNNWSPYLVPD
                     RTKVWLGLEYFCNESDPLWTRSDAEMTRLATEELARIGIIDAGDVLDATVVHVPKTYP
                     AYFGTYPRFHVVREYLDRFENLFLIGRNGMHRYNNQDHSMLAAMVAVDNLA"
BASE COUNT         1623 a         2889 c         2499 g         1746 t
ORIGIN      
        1 acttccattt cgctgctgtt gcgaaatggc tgtctgtccc catttcttcg tgagtcagtc
       61 cctggttttc tgtttccgcg ccaggtagaa cgtgcggtag cccggccgga tgctcggatc
      121 attcctcaac aacggagcgg catcccatgc gcgaattgtg tcgaagcccg catcgcgcag
      181 ggcgttgcgg atctcggacg cggtccagca gacctcttcg acgtgctcat gacgccgccg
      241 ccagtagcct ccttctcgga taaaccactc gacgtcgctc cacgcccgtt ccccggagcg
      301 atcacaaccg ccgtgcatca cgacggccac gccgggtttc tcgatgtaca aggtgagcgg
      361 ccagactccc ttgaaggccg cgcgattgtt gacgtcgaaa tagaaatgac caccggggcg
      421 cagtgcgcgc gcgacggttt tagtgacttt cgccaggtcc gtcctcctcg gaacatggtt
      481 gagggcgtcg aactcgcaca gcaccaggtc gacgggctcc ggaagccgga acgcccgcat
      541 gtcggcgtgg agcacttgca agggcacgcg agcccggcgc gccttctccc gagcgaggcg
      601 gcacatggtc ggggagaggt ccacaccgaa cgtcttgatt ccccggcgag ccaacgtcac
      661 cgcggttgat cccgtgccgc aggccagatc gcaggcggac accaccctgc gcagaagccg
      721 gccgagcacg tgcgtgcgcg gcgcctcacc ccaggagcgg gcaaacgaaa agaccctgtc
      781 gtaatacagc gccagccagt ggtaaggtcg gatggtttta gtgccaggca tggctcgaac
      841 caagcgctat tcattgtatg gaaaaccgtt tcaggggggt agcatcggcc agtcttgggt
      901 cttctaatcc ccttccggac cagaagcatg gatggctccg tatccttttc gaaccctcgt
      961 ggtaataaga agtgagccat gaccaccgcc gagctgcaat ccgtcttcga aggcaacagg
     1021 gatgcggtat tcggcttcgc ctggcggatg actggctcgc cagaaacggc tgaggacatt
     1081 gctcaggact gctttctgca attgctcaag tcgccggggc actatgacgg tatgcgcgga
     1141 cccattcgcc cttggctgct cggcgtcgcg cgcaatctga tcctaaagcg atggcgagag
     1201 gaagggcgtt ggactgcact cgatgaagag gaactcgcgg tagtcccaac atcaaacgat
     1261 tgtcgcgtgg atgagaaggt agcgcatgcc gttcaatcct tgcctcccct gcagcgcgag
     1321 gtgctgattc tgatcgagta cgaaggcctg acattggagg aagcgggccg tgcagtcggc
     1381 gctgaggtcg gggccgtcaa ggcgcggctg caccgtgccc gcaacaatct gcggcgcgtg
     1441 ctggcgccgc tcaggagtgt aaaccatgac ggatgacgag ttgagatcaa tgctgcggac
     1501 gtggcaagcg ccacccgctc cggaaacgtt gcggaagcgc gtgttccgcc gccggtggtc
     1561 cccattccga tggctggtcc agggggagat ccgggtgccg gttccactcg ccgttgccgt
     1621 gctctgcatg ctgatcttcg ctgccacacg tgtgtttcgg ccttccgcga ccagcttgtc
     1681 tgacttcgag caggtccagc agtttcaacc gagaatcgtg aggatggtcc atgaaactcg
     1741 ttagcctgat cctgcttacg ctttccgcgt acggcgcgca gttgatgagc ggcggcgcca
     1801 gcagtaacgg cgtctgggtg agctatgaaa cccggctgga gcccgactca ccgtcgattc
     1861 gaaagcacgg cggcggcaca ctgactgaga agaacgtgat caagcggcac ttgtgtaact
     1921 tcgacaacaa cacttacttt gggtatgact tggtcgcgga gccgctcggc ggcggccggt
     1981 accggctccg gtttgcaccc ctgacgatca caccgcggaa gatgagcgag atcttcagcg
     2041 aggcaaacgt ctcaaactgg actccgctgc cgctgcctcg tgaaccggcc acgttggaaa
     2101 tccaggcggg cgagactgta gccctcgatc tcttcgtcaa cccttccacg gggcagaaag
     2161 ttacggatta cctcacgata aaaggcgacg aacggcaggt tgtccacgtc gctgggcccg
     2221 cgcgcgactt cggccctgaa gacgcgacca tcgagatcag cttcccaaat gtgagcgtcg
     2281 atggaacgcc tctggtctcg tcccagggtg gggtctcggg acaggctgtc tggatcgatc
     2341 tacccgggca cgggcgcttc gtcttctcgc tcgcgccgca tccggacctc gggatgcaaa
     2401 aggcgggtga gattcgtgga acgacgatga cgtggcgcac aggcgggcac gagtacacga
     2461 ttacgacgga taagccgatt gcatccggat cgcgagccta caacctttat gtctttcaca
     2521 tcccccgaac tgccaactac ttcggcatgt ccgctggtgc caagccagac gatcccatcc
     2581 gcagcaggtg atgccggcag acatactgcc aacgggacgt tggtctgcaa cttgccgaac
     2641 agatacgccc cacgcgtgta tccggaaagc caggcttagc agcgaagcgg tccgatcttc
     2701 ccgctgggcc tcacactctc ggcgggtagt tcccggcccg agagtcacac ctatctcaga
     2761 gcgacgggct agttttcgcc atcgtagagt ttctccttcg cggctgcgcg gtacgctgtt
     2821 ttgatttctt tcggcaagaa gacgtacttc acgacctcat cgagaaggtg aggattgagg
     2881 aacaagggtg aattggtcgc agtgaaccca ccccgcaccc ggagccatct gcgtgtcctt
     2941 tttccaaaga agccattctg ccagagcaac ggttcccggg ccggatcttt cggtgcgtcc
     3001 acaatcttct caagacgccc gccctcgatc cggattattg gcgccggcaa accgtcactg
     3061 agtcttatct gccgcgggcc ttcgtcaagt gtgcaaaagc ggcgcaactc ccaaacagtc
     3121 ctatccagct taacgatatc gctgccgaat gaataaggcg acgtctcaag gtacctgaag
     3181 cggccatacg tgtcgatgta gtcaataaac ttctttgtcg gcgaggtgag gtcaagcgcg
     3241 atctttcctg cattggctat tttcgtgagc gcggcttcaa ggtcatgctt tatgtcaacg
     3301 gctggaatac ggttcaggag aagaatgcat ttaaggtact tctcaatcac ctgctggctg
     3361 gcccagaggt attgcagcgt cagcccagcg cggtaggcca ttcgggcaga aatgtagtcg
     3421 ccgtccccga cgtctcggaa acaacggata gcgaagttat tcagcaggat ttcgattttc
     3481 atgcgcgtac ggcggaggca cctctctgcc acgttctggg gcaataatcc ggacactgcg
     3541 actcccgaga atggctgtcc atcacactca ccgttggtcc gtcaatagaa ccgatactcc
     3601 ttcgggttca ccacgtcggg catctttgcc gggccgagtt cgtaactagc cggcgtgtag
     3661 tcgcgcttcg agttcagcgc ggcgtcccat tcgatcacct tcccactgta ggccgcctcg
     3721 cggcccagga tcgcggtcag cgtcgcctcg gccacgttgc gcgtttcgtt cagcggctta
     3781 ccgcgccgga tgctctcgat caggtccacg tgctcctgct cgtacgcctc cggcacctcg
     3841 ccctgatact tccaccctcg gtccgccacg atctggttct cgcagttgct cgtcccgttc
     3901 tcgcccacga ccgccgcccc gatccgcacg tcgcagttcg cgatctgccg gttctggctg
     3961 aacagccgcg ccccgcccgg gtactcgtac tcgaccgcaa aatggtcgaa tgtgtgcccg
     4021 aagtcgccgc ccggttcgtg gctcgcgcgc ccgcccacgc cgtacgcccg caccggatgc
     4081 tcgcccagca cccagttgat caggtccagc gtgtggatgt gctgttccac gatgatgtcg
     4141 ccggagagcc acgtgtaata cggccagttc cgatgctgcc actccaggtc gctccaacct
     4201 ccctctcgcg gaatggccca gatctcgccg acgttgaaat acgcccgcgc ctcgagaatc
     4261 ttcccgatcg cgccgtcccg cagccgccgg accgtctcca cctggtccag gctgtgccgc
     4321 cgcaccgtgc ccgccaccac accgagcccc ttcttctccg ccagcctgcc cgactccagc
     4381 acggagcgca cacccggcgc gtccaccgcg atcggcttct cggcgaagat gtgcttcccg
     4441 gcctccaccg ccgctctcat ctcccgcggc cggaactgcg gaggcgtcgc cagaatcacg
     4501 tagtccacct caggcatgcc gatcaccttc tcgtacgcgt caaagcccgt gaaacagttc
     4561 cggtcgctga tctcgactcc caccgtcttg agctgctgcc ggcactccgc cagccggttc
     4621 gtgaacaggt cggccagcgc cacaacctga acgttctgtg ccttcgcccg cgcgcccggc
     4681 accgcgtttt ccgtgtgata cccgcggccc ggcggcgggt agatcacgtc cgtcttcgct
     4741 tgcacgacgt ttagcgccgc ccccgtcccg cggccgccgc agccgatcac gcctacccgg
     4801 atcacctcgt ccgcggccag ccccttgctg acgaacggaa actgcacggc cgccaccgac
     4861 gacccccgca ggaagtcccg tctcgaaacg ccaccgttct ccatgattcc tcctctccgt
     4921 tctcactcac cacaccttca gcttcgccac cgtccatagt atctttgcgc cggccttcac
     4981 gctggcgatc gggtttcccg aaaccttctc ttcgccggcg aggcggcgcc ggtaagacac
     5041 ggggacctca agcacgcgca atccctgctg gagggccttg atctgcatct cgaccgtcca
     5101 cccgtaattg cggtcgcgca ttccgagcgc gcgcagggat tccaggcgga tcgcccggaa
     5161 cgggccgaga tccgcgtaac ggtaaccgaa cagaatccgg atcaggaagg tcgccagccg
     5221 gtttccgaac gcctggtgga ttcggaccga gccccgctcg gcgcgcccca tcacacgcga
     5281 acccagcacc aggtcggctc gcccctcggc aatcggagcg agcagcaggg ccgcttcctc
     5341 ggggtcttcg ctcgaatcgg cctgcatgaa agccacggcc tcggtgtccg gcggcacggc
     5401 ggccagcgcc gcaaggcacg ccgctccgta cccgcgctcg ggttcgctca ccacgatcgc
     5461 gccgtgttcc cgggcgacct ccgcggttcg gtccgccgac ccgttgtcgg ccacgatcac
     5521 ggtctggaac aggctccgcg gcacgcggtc gagcgtcgcc ccgaggcttt cttcctcatt
     5581 caaagcaggg atgatgactg ccgctctgag catcgcttat aattaaacat agctggactc
     5641 ggtcgcattc aactcatgac cggcgacaat ctaccgaggg aaactgacgg ccatcgacac
     5701 cacgcgccgc tctggcacgt cttcgcgtgg gcgcgcgatc tggtgatgtc cctcgtcatc
     5761 gcggccgtgg tgattctgtt catctaccag ccggttcaag tggaaggcac cagcatggag
     5821 cccgcgctcg tgaacgagga gcggatcttc attaacaagt tcacctaccg ctttggcatc
     5881 gagaagatcg ggcgcggcga catggtggtt tttcgttttc cgaacgatcc cagcaagtcg
     5941 tttatcaagc gcgttattgg gctgcctgga gactacgtcc tcattgagaa cgggcgcgtt
     6001 ttcgtcaacg accgcctcct cgaagaaccg tacctgcccg agtccttccg ggacaaagcc
     6061 tcttgggggc cgagcaggat cgcccaggat tactacttcg tcctcggcga tcaccgcgag
     6121 tcttccaatg acagccgcaa ctggggcgcg gtccatcgcg acctgattta cgggaaagcc
     6181 gtcttcgtct actggccgct cagcaaactc ggccccgttc gctgaggggg acgggctcgc
     6241 cgtccgatca gtaacaggat tgtcggaggt ccgttacgac tccttgccgt tgtacaaacc
     6301 cgcgacgtac ttctcatagc cgttgtacag cagtgtcggt cgccgctcca tcctcgggat
     6361 gaccttctcg aacgcctgcg accacctcgg gtgcggcttg ccgggattga tgttggagta
     6421 aaacccgtat tccaatggct gaagtttgtt ccagaacgtc gccggctgtt tagaaacgaa
     6481 ttcgatccgt acgatggcct tgatgctctt gtacccgtac ttccatggcg tcacgatgcg
     6541 gaccggcgct ccgttctgct tgggcaacgg tttgccgtag agccccgtca ccatcatcgt
     6601 cagcgcgttc atcgcctcgt ccatccgaag cgcctcgaag tacggccacg gataccacgg
     6661 cgcgcccacc atcccgggca tctccttggg ccggttgacg gtcacgaacc ggacgtactt
     6721 cgcctcgggc ttaggttcca ccgccttgat tagttcggag aacggaaatc ccgtccacgg
     6781 caccgccatc gaccatgctt ccacgcagcg atgacggtac aaccgttcct caaagggcat
     6841 ggtacgaagc aggtcatcga gatccaaggt ccgcggcttg ttcaccagcc ccgtgacctc
     6901 gattttccac ggacttacca cgaacgagcc aaccttgttt ttcactgcct gcttgtccgt
     6961 cgggtcaaat tcgtagaagt tgttaaagcc cgtcgccgcc cactcttccg tcaccggccg
     7021 gtccagcgtg aactccggat tccgtttcgc cgggtacggg cccgcggaag ccgacaccag
     7081 ccggtccact cccagaaaac ccgccgccag cagcaactgt ctgcgattca ggtagatatg
     7141 ctccggtgtc gcctgactct ccgggatttc ccagtcgcgt gggattcgaa tcaacatgac
     7201 tcctcccgca acccctcaat agccagatgc tagcatggta gaactttatt cctgaatgca
     7261 cgaaaaaacg gcaatcatcg tcggtgcagg acccgccggt ttgaccgccg cctacgaact
     7321 tctgtccaga accgctatcc gcccggtcgt cctcgagaaa agcgagttca tgggcggcat
     7381 cgcccgcacg gttaactaca agggctaccg gatggacatc ggcggtcatc ggttcttctc
     7441 gaagtccgac cgcgtcatga actggtggct cgacgtcatg ccgatgcagg ccgtccccgg
     7501 cgcgccaccc cccatcacct accagcggca gactcgcaca gtactcggct ccggaggagg
     7561 accagacccc gaccgcgagg accgcgtaat gctccttcgc aaacgccgct cgcgcatcta
     7621 cttcctccgc cggttcttcg actatcccat tcgtctcagc gcggaaaccc tcgctaagct
     7681 tggcctggtc cgcactgttc gaatcggtct gagctatctg aagagcgctg ccttccccat
     7741 ccgcgacgag cgcaccctgg agcagttctt tatcaaccgc ttcgggcacg agctctacct
     7801 cacttttttc aagtcttaca ccgagaaggt ttggggcgtg ccgtgcgacc ggatcagcgc
     7861 cgagtgggga agccagaggg tcaagggact ctctatcgcc cgctcggttc agcatttcct
     7921 gaagcaggcg ctgcgccgtc actcggacgt cgcacagaaa gacaccgaaa cctctctgat
     7981 cgagcagttc ctctacccca agctcgggcc cgggcaaatg tgggaggaag tcgcgcgaca
     8041 ggtttgtgga aaaggcggcg aggtcctgac gggctgggac gccgttcgtg tccgtacgga
     8101 cggctttcgc gtcactggta tcgaggccgt ccacgcctcg ggcgagcgcc ggctcttcga
     8161 gggagactac tttttctcga cgatgcccgt ccaggagctg gttcgcgcgc tggatcggcc
     8221 cgcgcctccg gatgtcgtcg aggtcagcga gggcctgatg taccgcgact tcatcactgt
     8281 cggcctgctc gtcggtggtc tcaaagtcaa ggaggatcat ccagacgggt caaaactcat
     8341 ccgcgataac tggatctaca ttcaggaacc cgacgttctc gtcggccgcc ttcagatctt
     8401 caacaactgg agtccctatc tcgtccccga cagaaccaag gtgtggctcg gcctcgagta
     8461 cttctgcaat gagtccgatc cgctctggac aagatctgac gccgagatga cccgcctcgc
     8521 caccgaggaa ctcgcccgta tcggaattat tgatgccggc gatgtccttg acgcgactgt
     8581 cgttcacgtc ccaaagacct acccggctta cttcggcact tacccgcggt ttcacgtggt
     8641 tcgggagtac ctcgaccggt tcgagaatct cttcctcatc ggccggaatg gcatgcaccg
     8701 gtacaacaat caggatcact cgatgctcgc ggccatggtc gccgtcgaca accttgc
//
LOCUS       DATIFQ010000521         5090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2671990, whole genome shotgun sequence.
ACCESSION   DATIFQ010000521 DATIFQ010000000
VERSION     DATIFQ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2671990"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VLE22_26335"
     CDS             <1..1124
                     /locus_tag="VLE22_26335"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB17997.1"
                     /translation="FVTRYLQWDFDNNPFCGPAEQRGYCHPESFKTITHLLYRNNRDG
                     TFQDVSKESGVGAVPGYGLGCAFNDYNHDGWPDLLAANDNVAQQLFKNNSDGTFEEVA
                     LLNGLAYNEDGGTFSGMGVDFNDFNNDGWPDVFVGALANERYSLFRNQKGSFTYVSEP
                     TGIGLMSNLHSGWGLKFIDFDNDGWKDLFVAQGHVMDTIEYTRPSTKYLEPMMLLRNV
                     RGKFEDVSKGRGTAFETPRASRGAAFGDLDNDGWPDIAVSVLNGPAVILRNEGGNGNH
                     WLIINAVGTVSNRDGIGARVRIVSESGLEQHSIVSPCGSYQSSNDRRVHFGLGKDTAV
                     KLVELTWPNGTVQMLENVKADQILTVKEPEAKPSGGASR"
     gene            1121..2875
                     /locus_tag="VLE22_26340"
     CDS             1121..2875
                     /locus_tag="VLE22_26340"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB17998.1"
                     /translation="MRGVLAASLATGLVVILAQPVFTQMMGGGRTAQPARPLPPGMKA
                     PQVRYEDLAAKAGLNGINVSGSDKQQAYIVENTGTGVAIFDFDNDGLMDLFFVNGDRF
                     DRNPNAPPTHHLYRNLGGLRFEDVTTKSGIAHTDWGQGVCAGDIDNDGFVDLYIGYWG
                     HNVLYRNQGNGTFRDETKERGLASTSRRWGTGCAFLDYDRDGNLDLFVAHYLAFDPSK
                     VAKPGEPGSCVWKGFPVVCGPMGLPPETMSLYHNEGGGKFRDVTEASGVGSAKAAGLS
                     VLTADFDNDGWLDVYVTGDSTPSLHFLNLRNGKFEEVGQFTGIAYNEDGHEQSGMGTS
                     AGDYDRDGNLDLVKTNFTDDIPNLYRNMGNGSFSEVTVAAGIAVHTEYVNWGTAFLDF
                     DQDGWKDIFIVSGHVYPDIDMRNVGQSFKQPRLLYWNRRDGQFFDISAQGGPGILARH
                     SSRGIALGDLDNDGDQEIVVVNMHESPSLLKNLGEKGNSILIQALTKTGRDAIGARLT
                     LTAGGKSQVDEVRSGGFYVSQGDFRVHFGLGGETKADLKIRWPDGSTESFSDLLAGYW
                     VTLRQGAGIMKTRKLGKE"
     gene            2953..>5090
                     /locus_tag="VLE22_26345"
     CDS             2953..>5090
                     /locus_tag="VLE22_26345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB17999.1"
                     /translation="MQKPLFRLAAAVLLAALSAHAYYPPVDTAGPLRVRIHEPALGLY
                     GAGGLAPLNQTGVPFTVTVSMENEGETELRGTLRLTVIDHWKVEPATPVPFAIDGKGR
                     GSFHFGVTIGEGTVNADYPVHAYAEFEHLGKRMVAHPILLLTPRLPNPPRARLPIEWK
                     PVPVPAVGAIGLWRLPVHRESAQVVSGEMFAGITGAEVSENAGPVQYGARVERGAARE
                     AVCMTLGPRPPAFRESLGFVRVEYPLQLPKTRPICLRFGIGGDATFRVRAAAFGTAGG
                     DGRVVFERTAAAKEWQDVEADLAGFAGQSIRLQLEAHGNPTATERACFAEPSLVTGTP
                     PGPPAFPPAEGQESKLLGAIEQSGTKYEVRLWPGRRGLLDSTVGFASGEKRLLFRGFR
                     ARVLGDALEDWRSAQELTGVKEERTAGRYRVRHSFRGWGDSFDLLGEIWTADNVLRAR
                     FWLENAPPAKPWLSVHIEEIAAGSWSEKAPRVYAGVGNVIENPQAFQMGFDGHRLASS
                     FVGFDFASGVSLVQAVDVPPDRLEVDPTRNVYTLNTPHAQTVTFIPSRDAWDAAIGWR
                     KVAGLRAAGGVSKLAGRFVFDLWAFDLGYAGGAKALQRAFRYGMTDAAVVWHNWQRWG
                     YDYRLPDIYPPNPKGGTPEEFAELARICRSNGVLFAPHDNYIDFYPDADDFSFEKIVF
                     QRDGTPRKAWYRSDLLAQSYRFRPDCVRPYL"
BASE COUNT          943 a         1584 c         1629 g          934 t
ORIGIN      
        1 tgttcgtgac ccgctacctc cagtgggact tcgacaacaa cccgttctgc ggcccggctg
       61 agcagcgcgg ctactgtcac cccgagtcat tcaagacaat cacccatctc ctctaccgca
      121 acaaccgcga cgggacgttc caagacgtat cgaaggagtc gggcgtcggc gccgtaccgg
      181 gctacgggct cggctgcgcg ttcaacgatt acaaccatga cggctggcct gacctgctcg
      241 ccgccaacga caacgtcgcc cagcagcttt tcaagaataa cagcgacggc acgttcgaag
      301 aggtcgcgct gctgaacggt ctcgcttaca acgaagacgg cggaactttc tccggcatgg
      361 gagtcgactt caatgacttc aacaacgacg gctggccgga tgtttttgtt ggcgccctcg
      421 ccaacgaacg ctactccctc ttccgcaatc aaaagggaag cttcacctac gtctccgagc
      481 cgaccggcat aggacttatg tccaatctcc actcagggtg ggggctgaag ttcatcgact
      541 tcgacaacga cggttggaag gatctgttcg tagcgcaggg acacgtgatg gacaccatcg
      601 aatacacgcg gccgtcgacg aagtacctgg agccgatgat gttgctacgg aacgttcggg
      661 gtaagttcga ggatgtctcc aagggccgcg gaaccgcgtt cgaaactccg cgagcatcgc
      721 gtggcgccgc ctttggcgac ctcgacaacg acggctggcc cgacatcgcc gtcagcgtgc
      781 tgaacggacc ggcggtcatt cttcgcaacg agggcgggaa cgggaatcac tggctgatca
      841 tcaacgcagt tgggacggtg agcaatcgcg acgggattgg cgcccgggtg cggatcgtct
      901 cggagtcggg gctggagcag cacagcatcg tgtcgccgtg cgggagttac cagtcttcca
      961 atgacaggcg agtccacttc ggtcttggca aggacacggc tgtcaaactt gtcgaactca
     1021 cgtggccgaa cgggacggtg cagatgctgg agaacgtcaa agccgaccag atcctgacgg
     1081 tgaaagaacc ggaagcgaag ccgtccggag gggccagtcg atgagaggtg tgcttgcggc
     1141 gagtctcgcg acgggtctcg tcgtcattct ggcgcagccg gtgttcacgc agatgatggg
     1201 cggtgggcgc acggcgcagc ccgcgcggcc gcttcctccg ggcatgaagg cgccccaagt
     1261 gcgctacgag gacctggcgg ccaaggcggg gctgaacggc atcaacgtgt ccggctcgga
     1321 caagcagcag gcgtacatcg tcgagaatac cggcaccggc gtggccatct tcgatttcga
     1381 caacgacggg ctgatggatc tgttctttgt caacggcgac aggtttgacc gcaacccgaa
     1441 cgcgccgccc acgcatcatc tgtaccggaa tctcggcggt ctccgcttcg aagacgtgac
     1501 aacgaaatcg ggcatcgccc ataccgactg gggccagggc gtatgcgcgg gcgacatcga
     1561 caacgacggt ttcgtcgacc tctacatcgg ctactggggc cacaacgtcc tctatcggaa
     1621 tcaaggcaac ggtacgtttc gcgacgagac aaaggagcgc ggcctcgcct caacaagccg
     1681 ccggtgggga accggctgcg cctttctcga ctacgaccgc gatggcaacc tcgacctttt
     1741 cgtcgcccac tacctcgcct tcgatccctc gaaagtggca aagccggggg agccgggtag
     1801 ctgtgtctgg aagggttttc cagtggtttg cgggcccatg ggtctgccgc cggagacgat
     1861 gtctctctat cacaacgagg gcggcggaaa gttcagggac gtgactgagg cgtctggggt
     1921 cggatccgcg aaagccgcgg gcctctccgt gctgacagcc gacttcgaca acgacggctg
     1981 gctcgacgtg tatgtcaccg gcgactccac acccagcctg cacttcctga atctacgcaa
     2041 cggcaagttc gaggaggtgg ggcagttcac cggcatcgcc tataacgaag acggtcacga
     2101 acagtccggc atgggcacgt ccgcgggcga ctacgaccgc gacggcaatc tggatctggt
     2161 taagacgaac ttcacggacg atatcccgaa cctctaccgg aacatgggga acggatcgtt
     2221 ctcggaggtg accgtcgctg caggtatagc ggtacatacg gaatacgtca actggggcac
     2281 cgcgttcctt gactttgacc aggacgggtg gaaggacatc tttatcgtca gcggccacgt
     2341 gtacccggac atcgacatgc gaaacgtcgg gcagagtttc aagcagccgc gcctgctcta
     2401 ctggaaccgc cgcgacgggc agttcttcga catttccgcg caaggtggtc cagggattct
     2461 cgcgcgccat tcctcgcgcg gtatcgcact cggggatctg gacaatgacg gcgaccagga
     2521 gatcgtggtc gtcaacatgc acgaatcgcc gtcgctgctc aagaatctcg gcgagaaggg
     2581 caactcgatt ctgattcagg ctctgaccaa aacggggcga gacgcgatcg gggcgcggct
     2641 gacgctcacc gctggcggga agagtcaggt agatgaagtc cgcagcggcg gcttctatgt
     2701 ttcgcaaggc gacttccgcg tccacttcgg gctcggcggc gagaccaagg ccgatctgaa
     2761 gatccgctgg ccggatggtt ccaccgagtc gttctccgac cttctcgcgg gctactgggt
     2821 gactctccgg cagggcgcag gtatcatgaa gacccgaaag ctcggcaagg agtagacgat
     2881 tgcacagcct gaaggctgtg ccacggccac tcgacaaccg aagtacactg gttcgtcgat
     2941 atggatctca gtatgcaaaa gcccttattc cgcctggcgg cggccgtcct gctcgccgct
     3001 ctttccgccc atgcctacta tcctccggtg gacaccgcgg gcccgctacg cgtccgcatc
     3061 cacgaaccgg cactcggcct ctacggcgcc ggcgggcttg cgcctctgaa ccagaccggc
     3121 gtgccgttca ccgtgactgt gtcgatggag aacgaaggag aaacggaact gcgcggcacg
     3181 ctgcggctga ccgtcatcga tcactggaaa gtcgagcctg cgacgcctgt gccgttcgcc
     3241 atcgacggaa agggcagggg gagctttcac ttcggggtca ccattgggga aggtaccgtg
     3301 aacgcagact acccggttca cgcgtacgcg gaattcgagc acctcggaaa gcggatggtg
     3361 gcgcatccca tcctgctgct gacgccgcgg cttccgaatc caccgcgcgc ccggctgcct
     3421 attgagtgga agccggtccc tgttcccgca gtgggagcga tcggcctgtg gcggcttccg
     3481 gtgcatcgcg aatcggcgca agtggtatca ggggagatgt tcgcggggat tacgggcgcg
     3541 gaggtttccg agaatgccgg tccggtgcaa tacggcgccc gggtcgaacg cggcgccgca
     3601 cgcgaggccg tctgcatgac tctcggccca cgcccaccgg cgtttcgcga atcgcttggt
     3661 ttcgtgcgcg tggagtaccc gcttcagctc ccgaagaccc gcccgatctg cttgcggttc
     3721 ggcatcggcg gggacgcgac cttccgcgtc cgcgcggctg cgttcggcac ggcaggcggc
     3781 gacggacgcg tggtctttga gcgaaccgcc gccgcgaaag aatggcagga tgtggaggct
     3841 gaccttgccg gattcgccgg ccagtcgatc cggctgcaac tcgaagcgca tggcaatccc
     3901 accgcgacgg aacgcgcttg cttcgccgag ccgtcgcttg tgaccggcac gccgcctggg
     3961 ccgccggctt ttccgccggc agagggtcag gaatccaagc tcctcggagc catcgagcag
     4021 tcgggaacga agtacgaagt ccggctctgg cccggccggc gcggtctcct cgactccact
     4081 gtcggtttcg ccagtggcga gaagcgattg ctgttccgtg gttttcgcgc gcgagtgctc
     4141 ggggatgccc ttgaagactg gcgctccgca caggagctga ccggcgtcaa ggaagagcgt
     4201 accgccggcc gttatcgagt gcggcacagc tttcggggct ggggcgactc gttcgatctt
     4261 ctcggcgaga tttggaccgc ggacaacgtg ctccgcgcgc gcttctggct cgagaatgct
     4321 cctcccgcca aaccatggct gagcgtgcat atcgaggaga tcgctgccgg ctcgtggagc
     4381 gagaaggcgc cgcgcgtgta cgccggcgtc gggaacgtga tcgagaatcc gcaggcgttc
     4441 cagatgggct ttgacggcca ccggctggcc tcgtcgtttg ttggcttcga cttcgccagc
     4501 ggcgtctctc ttgtccaggc cgttgacgtg ccgccggatc gtctggaagt cgaccctacg
     4561 cgtaatgttt acacgttgaa tactcctcac gcgcagaccg tgacgtttat tccttctcgg
     4621 gacgcctggg acgccgctat cggctggcgc aaagttgccg gactccgcgc ggccggcggt
     4681 gtgtcgaagc tagctggccg gtttgtcttc gacctgtggg cctttgacct cggctacgcc
     4741 ggcggcgcca aggcccttca gcgcgccttt cggtatggaa tgacggatgc ggccgtcgtg
     4801 tggcacaact ggcagcgctg gggttacgac taccgcctcc cggacatcta tccacccaac
     4861 ccgaagggcg gaacgccgga ggaattcgcc gagctggcgc gtatctgccg ctcgaacgga
     4921 gtgcttttcg ctccccacga caattacatc gacttttatc ccgacgccga cgacttcagc
     4981 ttcgaaaaga tcgtgtttca gcgcgacggc actccgcgga aggcctggta ccgctcggat
     5041 ctgctggcgc agtcctaccg cttccggccc gattgcgtgc ggccctatct
//
LOCUS       DATIFQ010000522         4711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2285681, whole genome shotgun sequence.
ACCESSION   DATIFQ010000522 DATIFQ010000000
VERSION     DATIFQ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285681"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VLE22_26350"
     CDS             <1..305
                     /locus_tag="VLE22_26350"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSB18000.1"
                     /translation="MAPHACEGPIGGIATLHLDAAIPNFLVQEVCSGVEPGMKEKVWE
                     EWLDFPAMRMVEGRFPLPEKPGLGFDLSEEAFRKYPFGGTRPMARVFHEDGSVAAW"
     gene            369..872
                     /locus_tag="VLE22_26355"
     CDS             369..872
                     /locus_tag="VLE22_26355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB18001.1"
                     /translation="MEREALLARLRERIVGYAAYKLTRDVAEDVAQEVMLVLHQKYPH
                     VGGMEELVPLALEIARFKITAHFRKTHRRGEDRLVPVDDLPLADAGPNPGEYAERKER
                     LERLEAALARLEGRCRELFRLKLQGKSFAEIQAILGAGSLNTVYTWDYRCRQRLLELM
                     GGREANR"
     gene            869..2029
                     /locus_tag="VLE22_26360"
     CDS             869..2029
                     /locus_tag="VLE22_26360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18002.1"
                     /translation="MKPEDIRKLLGGYATGTLTEEERQALFHAALEDQDLFNALADEH
                     AIKELFDDPATRARLLAGLSERKSAFRRLFDWIARPVPLGIAGTVAVAVLAAVVLIRP
                     AGKPPLQQVAMAPRSEAVAPAGVEGPSPEEPAKQDQRLTKTLAARKDAPATPAAPPAR
                     ATELKAKRNLEESGELASSMVPKPAELRDEAKAGVVGGVVGGVAGGYATQAPAPAPAA
                     APATVSSSVGKESRAKAASEVLSVRQEAVEENVRVARSAAETPPIVRYHLLRENIAGP
                     KEMEQDAAFRPEDRVRLAVEPATDGQLSVTLRSPGQPDLVLFDEPVRKGKSYTIPQEA
                     APPMSAGAGDQKLVLSFLPSLPSRSRSAALTMTRGAATQAPLVIEIPLKVQP"
     gene            complement(2047..3654)
                     /locus_tag="VLE22_26365"
     CDS             complement(2047..3654)
                     /locus_tag="VLE22_26365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HSB18003.1"
                     /translation="MTKLAASGKAIIYSTFLGGSQFTTPKAIAVDAGGSAFVAGSTTS
                     PDFPVTPGALQPALRSAMIAGYLDVDAFVVRLSLDAAAFLYSTYLGGSSSDDAAGIAV
                     DSLGNAYVTGNTSSLDFPLTAGAVQGPCESLTSCNRTGFLTKIHPAGTSTVYSTYLGG
                     TSYAVDVDSAGNAFVTGETGDGLTTARAFQPSFYGGLCVRYSASGVDPSGLYPCQDAF
                     AAGVAADGSSFLFSTYLNGYNIDRSNSIAVGPGNTVFVAGSGELSAATLDSAWPQGNA
                     FVVRLRRGVTPISFTRESITNAASFATGLPPPGGLATIFCSNLIGIRGVVRATSFPLP
                     HELAGVSVFVSGFPAPLLAVADVNGRQQINFQVPYEIDGLSSADVVVKQNGNWAMVTV
                     RFDMSPPGVFQWDGGYGAIQHAADYSLVTPSNPAVRGEVVIVYATGLGPVEPMPETGL
                     AVPVSPLSETVEQPTVTIGGQPAEVLFSGLAPGFAGLYQLNVRIPPDAPEGDAELRVS
                     LPTIRDAWVWEIKLVPRISQPVRISIK"
     gene            complement(3697..4386)
                     /locus_tag="VLE22_26370"
     CDS             complement(3697..4386)
                     /locus_tag="VLE22_26370"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HSB18004.1"
                     /translation="MPVRRVCFRDIATRLCILFGCCIAELVAAPPIPFAPGLFEEATS
                     PVQYAVNLGNPPFTQPFIVGGNQLDTVVGIAVDTAGNAYIVGTTYSPAFPEAGPCPQI
                     GGNPNWGFLAKLKPNGNEFAYLKILPFYPAAISVDPAGNVYITGEAGGGPIDPSPPRL
                     VRARLYDDDLDVFVAKYDPSGNLLWHSYVGGSKEDRVTAIAVDPHGYVYVTGWTRSPD
                     FPTSPNAVGDR"
     gene            complement(4479..>4711)
                     /locus_tag="VLE22_26375"
     CDS             complement(4479..>4711)
                     /locus_tag="VLE22_26375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18005.1"
                     /translation="RDGGQPGRCGESHELSSSAFEIASRTGICREDGDPFSGGLRGIS
                     AGFPENVNLGNCNSKKYQGYVSMFQIIRADFR"
BASE COUNT          940 a         1496 c         1479 g          796 t
ORIGIN      
        1 cgatggcgcc ccatgcgtgc gagggcccga tcggcgggat cgccaccttg cacctcgacg
       61 cggcgatccc gaacttcctt gtccaggagg tttgcagcgg agtcgaaccc ggcatgaagg
      121 agaaggtctg ggaagaatgg ctcgatttcc ccgccatgcg catggtcgag gggcgcttcc
      181 ccttgccgga gaagccggga ttgggattcg acctgagtga ggaggcgttc cggaagtacc
      241 cgtttggtgg cacgcgcccg atggcccggg tctttcacga ggacggctcg gtggctgcgt
      301 ggtgaacggt tttcggcggc gagcgaacgg aagaaaacca ttcggtgtct aatagttggg
      361 gaacgactgt ggaacgagag gccctcctcg cccgcctgcg cgaaaggatt gtcgggtacg
      421 cggcatataa gcttacgagg gacgtcgccg aggacgtcgc ccaggaggtg atgctggtcc
      481 tccaccagaa gtacccgcac gtcggcggaa tggaggaact ggtgccgctc gcgctcgaaa
      541 tcgcgcggtt caagatcacg gcgcatttcc gcaagacaca caggcgggga gaggacaggc
      601 tcgttccggt ggacgatctg ccactcgccg acgcaggccc gaaccccggc gagtatgcgg
      661 aaagaaagga gcgactcgaa cggctggagg cggcgctggc gcggctcgag ggacgctgcc
      721 gggaactgtt ccggctaaaa ttgcagggta agagcttcgc ggagattcag gcgatcctcg
      781 gagcgggttc cctgaacacc gtgtacacgt gggactaccg atgccgccag agactcctgg
      841 aattgatggg cggccgggag gcgaaccgat gaagcccgaa gacatccgaa agcttctggg
      901 aggctacgcc acgggtacgc tcaccgagga ggagcggcag gcgctgttcc acgccgcgct
      961 cgaagaccag gatctgttca acgcgctcgc tgacgagcac gcgatcaagg agctttttga
     1021 cgacccagcc acccgcgcga ggctccttgc cggattgtcc gagcgaaagt cggctttccg
     1081 gcggctgttt gactggatcg cccggcccgt cccgctggga atcgccggga ctgtcgccgt
     1141 cgcggttctc gccgccgtcg tactgatccg gccagccggc aagcccccgc tccagcaagt
     1201 cgcaatggcc ccgcggtccg aggcggtggc tccggcgggc gtggaagggc cgtctccgga
     1261 agagccggca aagcaggatc agcgtctcac gaagacgctt gcggcccgga aggacgcacc
     1321 cgcaacaccg gccgctcctc cggcacgggc gaccgagctg aaggcgaaac gaaacctcga
     1381 agagtcaggc gagcttgcct cgagcatggt ccccaagccc gccgagctgc gcgacgaggc
     1441 caaagccggg gtcgtcggtg gggtggtagg cggtgtggcc ggtgggtatg caacccaggc
     1501 accggcgcca gcgcccgccg cggcacccgc gaccgtctcc tccagcgttg ggaaggagtc
     1561 ccgcgcaaag gcagcatcgg aagtgctgtc cgtgcgccag gaggcagtcg aggagaatgt
     1621 gcgggtcgcg cgctcggcgg ccgagacgcc gcccattgtc cgttaccatc tgcttcgcga
     1681 aaatatcgct gggccgaagg aaatggagca ggacgccgcg ttccgtcccg aagaccgggt
     1741 ccgtctcgcc gtcgaacccg ccaccgacgg ccagctttct gtaacgctcc gttcgccagg
     1801 ccagcccgac ttggttctat tcgacgaacc ggtacggaag ggcaagtcat acacgattcc
     1861 ccaggaagcc gcgcccccca tgagtgccgg cgcgggagac cagaagctgg tcctgtcctt
     1921 cctgcccagc ctgcccagcc ggtcccggtc cgccgccctc accatgaccc gcggcgcggc
     1981 gacccaggcc ccgctggtca tcgaaatccc cctgaaggtc cagccgtagc ccaccgctga
     2041 aagttcttac ttaatcgaaa ttcgtacggg ctggctgatg cgcggaacga gttttatctc
     2101 ccatacccag gcgtctcgaa ttgtcggtag gctgacccgc agctcggcat ccccttccgg
     2161 tgcgtccggt ggaatccgga cgtttaactg gtacaggccg gcaaagcccg gggcgagtcc
     2221 ggagaacaga acctccgccg gctggccccc gatggtgaca gtaggttgct ccaccgtctc
     2281 cgagagtggg ctcacaggca cggcgagacc ggtctccggc attggttcga cgggccccag
     2341 tccggtggcg tacacgatga ctacctcacc ccttaccgcc gggttggaag gcgtgaccag
     2401 actatagtcc gcggcgtgtt gaatcgctcc gtagccgcca tcccactgga acactcccgg
     2461 cggcgacatg tcgaaccgaa ccgtcaccat ggcccagttc ccattctgct tcacgaccac
     2521 atccgccgaa gagaggccgt caatttcata tgggacctgg aaattgatct gctgccgtcc
     2581 gttcacgtcc gcaacggcca gcaacggggc cggaaagcca ctgacaaaaa ccgacacccc
     2641 agccaactcg tggggaagtg gaaaactcgt tgcgcggacg acaccacgga tccctatcag
     2701 gttcgaacag aagatcgtcg ccaggccccc cggcggcggc agcccggtcg cgaagctggc
     2761 ggcgttcgtg atcgactccc gagtgaagga aatcggcgtg actccgcggc gtaggcgaac
     2821 gacgaacgcg ttaccctgcg gccacgccga atcgagagtg gctgcgctca actcacccga
     2881 gccggcaacg aacaccgtgt tcccgggtcc caccgcgatg ctgttgctcc gatcgatgtt
     2941 gtagccattg aggtaagttg agaacagaaa gctggatccg tcggccgcga ctccggcggc
     3001 gaaggcatcc tggcatgggt acaacccgct tggatcaaca cctgaggcac tgtatcgaac
     3061 acagaggccg ccgtaaaagc tcggctggaa tgcccgcgcc gtcgtcaggc cgtcgccggt
     3121 ctcaccggtc acaaatgcgt tccccgccga gtccacatcg acggcatagc tcgttcctcc
     3181 aagataggtc gaatatactg ttgacgttcc cgcaggatgg atcttagtga ggaacccggt
     3241 cctgttacat gaagttaggc tctcacaagg cccctgaacc gcgcctgcgg tcagcgggaa
     3301 atcgagggaa ctggtattgc cggtcacgta ggcgttcccc aacgagtcca cggcgatgcc
     3361 ggctgcgtcg tcggatgagc ttccccccag gtaggtggaa tacaggaatg cggctgcgtc
     3421 cagactgagc ctgaccacga acgcatcaac atccagatag ccggcgatca ttgcagaccg
     3481 cagagcaggc tgcaaggcgc ccggcgtcac ggggaagtcc ggcgatgtgg tcgagccggc
     3541 gacaaacgcg ctgcctccgg cgtcgacggc gatcgccttg ggagtcgtga actggctgcc
     3601 gcccaggaag gttgagtaaa tgattgcctt tccggaagcg gccagtttgg tcacgaagcc
     3661 tgcctctccc gacaagccgt aggcgcctgt agccggttac cgatcaccaa cagcattcgg
     3721 actggttgga aaatccggcg atcgggtcca gccggtcacg taaacgtaac cgtgcggatc
     3781 cacggcaatc gcagtgaccc gatcctcctt gctgccgccg acgtaagaat gccagagcaa
     3841 gttgccgctc gggtcatact tggcgacgaa gacgtccaag tcatcgtcgt acaatcgagc
     3901 ccgcacgagt cgcggcggcg atggatcgat cgggccccca cctgcctccc cagtaatgta
     3961 cacgttgccg gccgggtcga ccgaaatcgc cgcgggatag aacggaagta tctttagata
     4021 ggcgaactcg ttcccgttcg gcttgagctt cgccagaaaa ccccaattcg ggtttccgcc
     4081 gatctgagga catggacccg cctcggggaa cgctggtgag taagtcgtac cgacaatgta
     4141 tgcgttgccg gcggtatcaa ccgcaatgcc taccacggtg tccagttgat tcccgccgac
     4201 gatgaagggc tgtgtgaacg gcgggttccc aagattcacg gcgtactgga ccgggcttgt
     4261 cgcctcctca aacaggccgg gcgcgaaggg gatgggcggc gccgcaacta gctccgcgat
     4321 gcagcagcca aacaggatac acagtcgagt cgctatgtct cgaaagcaga ccctccgaac
     4381 cggcatcgct ctcctccgtt cggcttccaa caccccaccg ccccagggta gggcatttgt
     4441 agcccacggt tggattgtat ccgtgacttt aagaatcgtc aacgaaaatc cgctcttatt
     4501 atctgaaaca tactcacata accttggtac ttctttgaat tacagttgcc aagattcacg
     4561 ttctcgggga atcccgccga aatcccccgt agtccgccgc tgaaaggatc accgtcctcg
     4621 cggcatatcc ccgttcgaga agcaatctcg aaggccgagg aggacagttc atgagactca
     4681 ccacaccgac cgggctggcc gccgtcgcgt t
//
LOCUS       DATIFQ010000523         7226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_565638, whole genome shotgun sequence.
ACCESSION   DATIFQ010000523 DATIFQ010000000
VERSION     DATIFQ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565638"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..2410
                     /locus_tag="VLE22_26380"
     CDS             263..2410
                     /locus_tag="VLE22_26380"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB18006.1"
                     /translation="MGSVPTEGRAIRFGVYEVDLAAGELRKRGRAVKLQGQPFQILAM
                     LLRRPGEVVSREELQQALWPSGTFVEFEHGVNTAIRKIREALGDSADNPRFIETLPRK
                     GYRFIAPVNGIRPALQAPARRSVVMWALASIAAAAVAGAVWLYLSRFDQPAPSPLVVP
                     LTTYPGSECDPSFSPDGNQVVFAWDGGDKADAAGLYVKVIGTDAPVRLAAGRAFAPAW
                     SPDGRWIVFARALPGSETIVQVLLIPATGGFERKLAEIKAAPSPDFLPQTIDDPATRA
                     EFWPPFSWDSTAKWIAGTAKPSESEPTAVFLLSATTGEKRKLTSPPTGIPGDGLPAFA
                     PDGRALLFVRTRGAHIRDLYLVELSRDFRPNGEPVRLTFENRFVGKPVWMPDGRSILF
                     PAGSVHNPSLWRLALTRLPSRPGKLERLAFAGFGVTSPAVSRQDSLAYSLAPFDTDIW
                     RLELDDTGRAAKLATKLIVSTRIDHTPQYSPDGRRIAFGSDRSGNHEIWVSNSDGSNA
                     MKLTSFDGPYVADPRWSPDGRFIAFGASGEQSTVYAISSDGGTPGRVVAGSLDGWSRD
                     GKWIYFSSDLKGNHELWKVPVNGNPVQVTRRGGTGGVESPDGRFLYYKKTANGVTSLW
                     KAPVEGGEEVRILDSFDDRYFVVAERGIYFLTSEGKRIQFYSFATRKVETVAEFQKFN
                     CAWGLSVSPDGRQLLYSFPEEHDSDLMLVENFR"
     gene            2407..4530
                     /locus_tag="VLE22_26385"
     CDS             2407..4530
                     /locus_tag="VLE22_26385"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB18007.1"
                     /translation="MSAAEPGSSTNDTVHFGLFEVDLVAGELRKRGRAVKLQGQPFQI
                     LAMLLRRPGEVVSREELQQALWPSGTFVEFEHGVNTAIRKIREALGDSADNPRFIETV
                     PRKGYRFIAPVNGTHPAQQPARRLVVMWVLAAVAAAALAGAVWLSLSRSDQPAPSPLV
                     VPLTTYPGSESQPSFSPDGSQVVFVWDGGGKADAAGIYVKVIGTDVPVPLAAGDAFAP
                     AWSPDGRWIVFARTLPGSETKLQVLLIPATGGPERKLAEIEARGNATAELWCQFSWDP
                     TAKWIAVPDKPNDNEPTAVFLLSAVTGEKRRLTSPPAGLAGDDLPAFSPDGRALLFVR
                     AVSESHIRDLYLIELSRDFRPSGEPVRLTFENRFVGKPAWMPDGRSILFPAGSAHNPS
                     FWRLALNRLPWRPSKLERMAFAGFGVISPAISRKDSLAYGVWPKDTDIWRLELDDTGR
                     AAKPATKLIVSTRIDHTPQYSPDGRRIAFGSDRSGNHEIWVSNSDGLNPMKLTSFDGP
                     YVADPQWSPDGRFIAFGAAGEQSSVYVISSDGGVPRRVVAGWTGGWSRDGKWIYFSSD
                     LKGNHELWKAPVNGGNPVQVTRSGGTGGLESPDGRFQYYEKTANGVTSLWKAPVEGGE
                     EVQILDSLSDQYFVVAERGIYFFSSEGKRIQLYSFATRKVETVAELPKFNWAWGLSVS
                     PDGRQLLYSLPEEHGSDLMLVENFR"
     gene            complement(4571..5245)
                     /locus_tag="VLE22_26390"
     CDS             complement(4571..5245)
                     /locus_tag="VLE22_26390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18008.1"
                     /translation="MKKLIGSWCLLGLAVAVQAETPDPELRFQVLVYNYAGVSLGTLS
                     HAKQEAGRIYRQIGVGVEWRDCPLPAEEGQPEPLCGLPSAPTRLSLRLLSNSMGQRVT
                     PRYDVLGWAFLPANGGFGLTANVFVGRAWQRADVCPLSRSLVLGLLMAHELGHLLLGR
                     PEHAVSGAMSVPLMKGCPESMSRGRKSLPRDVFFLSQQAGQIRANITARIAGERAARA
                     APITSR"
     gene            5454..>7226
                     /locus_tag="VLE22_26395"
     CDS             5454..>7226
                     /locus_tag="VLE22_26395"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB18009.1"
                     /translation="MGSVSTEGRAIRFGVYEVDLAAGELRKRGRTVKLQGQPFQILAM
                     LLRRPGEVVSREELQQALWPSGTFVEFEHGVNTAIRKIREALGDSAENPRFIETLPRK
                     GYRFIAPVDGREIRESPRRGRAWVLALALCAALLGGLGVWSFMRTGTKPEAAPVPIPL
                     TSFPGREGSPSFSPDGSQVAFAWQHEGQETRDICVKVIGLEETRCLTSGPADEHSPAW
                     SPDGRYIAFIRDLGEEKGGIFLIPAIGGPERKVAEGRFPPWFRSRLAWHPGGRWLVTS
                     IRRSASELSALFGISVESGDLLRLTSPIGWADEQGVVAPNGGAVAFSRCVEDQVCDIY
                     VLEVSEDLRPKGEPRRITSFRDRVATAPAWTADSRAIVFTSGSRTNPSLWKVAVSGPG
                     WKPGNPERMTFAGYSVWWPAVSRQGRLAYALFPKGVDIWRLELDASRPGTPSGGHGKP
                     PLKLIPSTRVGHTPEYSPDGRRIAFGSDRSGNHEIWVANSDGSNVMQLTSFDGPYTAG
                     PHWSPDGRSIAFASSAGGNGDVYIISLDGGKPKRLTTNASDDGPSAWSRDGKWICFYS
                     DRTGEPQVWKVRAEGGGEVQVTRNG"
BASE COUNT         1291 a         2097 c         2392 g         1446 t
ORIGIN      
        1 agcgtcttca ttgatctcgc ctccatggcg agacccaaga attcgctagt gagaggtaac
       61 cttcaatgca cggcggctgg agcttgggtc aattgcaccg ggagcgctcc taaagagcca
      121 actcattgcc aggaaacgaa ttgccggcgc acataaactc gcgggttagg cgaaaaagtt
      181 cgctgggcgc ccgctccctc acggtcgcgg ttcttcaact gcgggtttcg gtgttagaat
      241 cgaagccaaa ctgaggcggg ccatgggttc cgtaccgacc gaagggcgcg cgattcgatt
      301 cggtgtctac gaggtagacc tcgctgccgg cgagttgcgc aagcgggggc gggcggtcaa
      361 gcttcaggga cagccgtttc aaattctggc gatgctgctg cggcggccgg gcgaggtggt
      421 cagcagggaa gaacttcagc aggcgctgtg gccgtcgggc acgttcgtgg agtttgagca
      481 cggggtgaac acggcgatcc ggaagatccg ggaggcgctg ggggactcgg cagataatcc
      541 gcggttcatt gagaccctgc cccggaaagg gtaccggttc attgcgccgg tgaacgggat
      601 tcgccctgcc ctgcaggcgc cggctcgccg ctctgtcgtc atgtgggctt tggcctcgat
      661 tgccgctgcc gccgttgccg gcgctgtctg gctctacctg tcacgtttcg accagcccgc
      721 gccttctccg ctggttgttc ccctgactac ctacccgggc tcagagtgcg atccgagttt
      781 ttcaccggat ggcaaccaag tggtttttgc ttgggatggc ggagacaagg cggatgcggc
      841 cggtctgtat gtcaaggtga tcggcacaga cgctccggtc cgactcgccg ccgggcgcgc
      901 gtttgcgccg gcctggtcgc cggatggtcg ctggattgtc ttcgcacgcg ccttgccagg
      961 gtcggagacc atagtgcaag tgttgctgat cccggccacg gggggctttg agcgcaagct
     1021 ggcggagatc aaggctgcac cctctccaga ctttctgcca cagaccatcg acgatccggc
     1081 gaccagagcg gagttttggc ctccgttctc gtgggattcg actgcgaagt ggatcgccgg
     1141 caccgcaaag cccagcgaga gtgagcccac tgccgtattt ctgctgtctg ccacgacggg
     1201 ggagaagcgg aagttgacgt cgccaccgac tggaatcccc ggtgatggct tgcccgcgtt
     1261 tgccccggat ggccgtgcgc tgttgtttgt caggactcgt ggtgcccaca tccgcgatct
     1321 ctacctcgtc gaattgtcca gggactttcg gcccaacgga gaaccggtgc ggctgacgtt
     1381 cgagaatcga ttcgttggaa aaccagtgtg gatgccggac gggcgctcga ttctctttcc
     1441 tgcgggttct gtccataatc cgtctctctg gcggctggcg ctgacccggc tcccgtcgcg
     1501 gcccgggaag ctggagcggc tcgctttcgc cggattcggc gtcacctctc ccgccgtatc
     1561 gcggcaggac tcgctagcct actctctggc gccttttgat accgacatct ggcgccttga
     1621 gttggacgac acaggccgcg ccgcgaagct tgccacgaag ctcatcgtct ccacgcggat
     1681 cgaccacacc ccgcaatact ctccggacgg ccgccggatc gcctttggtt cggaccggtc
     1741 ggggaatcac gaaatctggg tgtcgaacag cgacggctcg aatgcaatga agttgacctc
     1801 ctttgacggg ccgtacgtgg cagatccgcg atggtctccg gatggacggt tcattgcgtt
     1861 cggagcctcc ggtgagcaat cgacggttta cgcaatcagt tccgatggcg gcacgccagg
     1921 gcgtgtggtt gccggctcac tggacggctg gtcccgagac ggcaagtgga tctatttcag
     1981 ctctgatctt aagggaaacc acgagttgtg gaaggtgccg gtgaatggca acccggttca
     2041 ggtcacgaga agaggcggaa cggggggcgt cgaatctcct gacggccggt tcctctacta
     2101 caagaagacg gcaaacggag ttaccagcct gtggaaagcg cccgtcgagg gtggtgaaga
     2161 agtccggatt ctcgactcct tcgacgaccg gtatttcgtc gtggccgagc gagggatcta
     2221 cttcttaacc tccgagggga agcggatcca gttctatagt ttcgcgacgc gaaaagtgga
     2281 gacagttgcg gaatttcaaa agttcaattg cgcgtggggt ctctccgtct ctcccgacgg
     2341 ccgccagttg ctgtactcat tcccggagga gcacgacagc gacctgatgc tggtggagaa
     2401 ttttcggtga gcgccgcgga accaggctcg agcacgaatg atacggttca cttcgggttg
     2461 ttcgaggtag acctcgttgc gggtgagttg cgcaagcggg ggcgggcggt caagcttcag
     2521 gggcagccgt ttcagattct ggcgatgctg ctgcggcggc cgggcgaggt cgtcagcagg
     2581 gaggaactcc aacaggcgct gtggccgtcg ggcacgttcg tggagttcga gcacggggtg
     2641 aacacggcga tccggaagat ccgggaggcg ctgggggatt cggcggacaa tccacgattc
     2701 atcgagacgg tgccgcggaa ggggtatcgg ttcatcgcgc cggtgaacgg gacccaccct
     2761 gcccagcagc cggctcgccg ccttgtcgtc atgtgggttc tggccgcggt tgccgctgcc
     2821 gccctcgccg gcgctgtctg gctctcccta tcacgttccg accagcccgc gccttccccg
     2881 ctggttgttc cgctgactac ctacccgggc tcagagtcgc aaccgagttt ttctccggat
     2941 ggcagccaag tggtttttgt ttgggatggc ggaggcaagg cggatgcggc cggtatctat
     3001 gtcaaggtga tcggcacaga cgtgccggtc cctctcgctg ccggggatgc gtttgcgccg
     3061 gcctggtcgc cggatggtcg ctggattgtt ttcgcacgca ccttgccagg gtcggagacc
     3121 aaactgcagg tgttgctgat ccccgccacg ggaggccctg agcgcaagct ggcggagatc
     3181 gaagctagag gaaacgccac cgcggagctt tggtgccagt tctcctggga cccgactgcg
     3241 aagtggatcg cggtccccga taaacccaac gacaacgagc ccactgcggt gtttctgctc
     3301 tctgccgtga ccggcgagaa gcggaggttg acctctccgc cagcgggcct cgccggagat
     3361 gacttgcccg cattttcgcc ggatggccgt gccctcttat ttgtccgggc tgtctctgaa
     3421 tcccatatcc gcgatctcta cctcatcgaa ttgtccaggg acttccggcc cagcggggaa
     3481 ccggtgcggc tgacgttcga gaatcgattc gttggaaaac cagcgtggat gccggacggg
     3541 cgctcgattc tcttccctgc gggttctgct cataatccgt ctttctggag gctggcgcta
     3601 aatcggctcc catggcggcc cagcaagctg gaacggatgg ctttcgccgg attcggcgtc
     3661 atctctcccg ccatatctcg gaaggactcg ctggcgtacg gtgtgtggcc taaagatacg
     3721 gatatctggc gcctggagtt ggacgacaca ggccgcgctg cgaagcccgc cacgaagctg
     3781 attgtctcca cgcggatcga ccacacccca caatactctc cggacggccg gcggatcgcc
     3841 tttggttcgg accggtcggg gaaccacgaa atctgggtat cgaacagcga cggcttgaat
     3901 ccaatgaagt tgacctcctt tgacgggccg tacgtggcag atccgcaatg gtctccggat
     3961 ggacggttca ttgcgttcgg agccgccggt gagcaatctt cggtttacgt aatcagttcc
     4021 gatggcggcg tgcctcggcg tgtggttgcc ggctggacgg gcggttggtc ccgagatggc
     4081 aagtggatct atttcagctc tgatcttaag gggaaccacg agttgtggaa agcgccggtg
     4141 aatggaggca acccggttca ggtcaccaga agcggcggaa cggggggcct cgaatctccc
     4201 gacggccgat tccaatatta cgagaagacg gcaaacggag ttaccagcct gtggaaagca
     4261 cccgtcgagg gtggtgaaga agtccagatt ctcgactcct tgtctgacca gtatttcgtc
     4321 gtggctgagc gggggatcta cttcttctcc tccgagggga agcggatcca gctctacagc
     4381 ttcgcgacgc gaaaagtgga gacagttgcg gaacttccaa agttcaattg ggcatggggt
     4441 ctctccgtct ctcccgacgg ccgccagttg ctgtactcac tcccggagga gcacggcagc
     4501 gacctgatgc tggtggagaa tttccggtga ggatagggca atcttagtaa acaccaacga
     4561 cacggcttga ctatctggag gtgatcggag ccgctctggc ggctcgttcg ccagcgatcc
     4621 gggcggtgat gttagcccgg atttgcccgg cttgctgtga caggaaaaac acgtctcggg
     4681 gcaagctctt cctgcctcga ctcatgctct cggggcaccc tttcatcagc gggacgctca
     4741 tggcgccaga cactgcgtgc tcgggcctcc cgagcagcag atggcccagc tcgtgagcca
     4801 tcagaaggcc gagaaccagc gaacgggaca gcgggcatac gtccgcccgt tgccatgcgc
     4861 ggccgacaaa cacgttggcc gtcaggccga agccgccgtt cgccggcaaa aaagcccacc
     4921 caagaacgtc ataacgcggc gtgacgcgtt ggcccatgct gttggatagt agccggagac
     4981 tgagcctggt gggcgcactg ggtagcccac agagcggttc gggctgcccc tcctccgccg
     5041 gaaggggaca atcccgccat tccacgccga caccgatctg ccggtagata cgacccgctt
     5101 cctgcttcgc gtgactcagt gtcccaagag acacaccggc gtagttgtaa acgagaacct
     5161 ggaacctcaa ttcaggatct ggcgtttccg cctggaccgc tacggcaagg cctaacaggc
     5221 accaacttcc aatcagcttc ttcattgatc ccgccccgtg gtgggatcaa gaattggcta
     5281 gtgcaaggta accttcaagg cacggcgtct ggagtctggg tcaatcgcgc cgggagcact
     5341 cctaaagagc caactcattg ccggtgagcg aattgccggc gcatataaac tcgcgttggg
     5401 tctttgtttc ctcgggttca ggtgctaaaa tcgaagccaa actgaggcgg gccatgggtt
     5461 ccgtatcgac cgaagggcgc gcgatccgat tcggtgtcta cgaggtggat ctcgctgcgg
     5521 gcgagttgcg caagcggggg cggacggtca agcttcaggg gcagccgttt cagattctgg
     5581 cgatgctgct gcggcggccg ggcgaggtgg tcagcaggga agaactgcaa caggcgttgt
     5641 ggccgtcggg cacgttcgtg gagtttgagc acggggtgaa cacggcgatc cggaagatcc
     5701 gggaggcgct gggggattcg gcggagaatc cgcggttcat cgagacgctg ccgcggaagg
     5761 ggtatcggtt catcgcgccg gtggatgggc gggaaatccg cgagtcgcca cgtcgtgggc
     5821 gcgcgtgggt tctcgcattg gcgctttgtg cggcgctgct cggcggcttg ggggtctggt
     5881 cgttcatgag aaccggcaca aaaccggaag cggccccggt tccgattccg ctgacaagct
     5941 ttcccgggcg cgaggggtcg ccgagctttt ctcccgacgg cagccaagtc gcatttgcgt
     6001 ggcagcatga ggggcaagag acgcgcgaca tctgtgtgaa ggttattggc ctggaagaaa
     6061 cgcggtgcct caccagcggt ccagcagacg aacacagccc ggcttggtcg ccggacgggc
     6121 gttacatcgc attcatccgg gacctggggg aggaaaaggg cggcatcttc ctgattccag
     6181 ccatcggtgg gccggagcgc aaggtggccg aaggacgctt cccaccatgg tttcggagtc
     6241 gccttgcctg gcacccgggt ggccggtggc tcgtcacatc catcagaaga tcagccagcg
     6301 agctttccgc tttgtttggg atttcggtgg agagcggaga tctgctgagg ttgacgtcgc
     6361 cgatcggttg ggctgacgag cagggagttg ttgcgccaaa tggaggcgct gtagcgttct
     6421 cccgctgtgt tgaggaccag gtctgcgaca tctacgtgct ggaggtttcc gaggacctcc
     6481 ggcccaaggg ggagccgcgg cgcatcacgt cttttcggga cagggtcgcc acggcgccag
     6541 cctggacggc ggatagccgg gcgatcgttt tcacttccgg ctcccgtacc aacccaagct
     6601 tatggaaagt tgcggtttcc gggcccggat ggaagcctgg aaatcctgaa agaatgactt
     6661 ttgccggcta tagcgtctgg tggccggcag tctcccggca agggcgcctg gcctatgcgt
     6721 tgtttcccaa aggggtggac atctggcgcc tcgaattgga tgcatcgcgc ccaggcacgc
     6781 cgtccggcgg gcatggcaag ccgccgctca agctgattcc ctccactcga gtggggcaca
     6841 ctccggagta ttcgcccgat ggccggcgga tcgcattcgg ctcggaccgc tctgggaacc
     6901 acgagatctg ggtggcaaac agcgacgggt cgaatgtcat gcagttgaca tctttcgatg
     6961 gaccgtacac cgctggtcct cactggtcgc cagacggccg gtcgattgct ttcgcctcga
     7021 gtgcgggcgg taacggagac gtctacatca tcagcttaga cggcggcaag cccaagcgcc
     7081 tgacaaccaa tgcctctgat gatgggcctt ccgcctggtc gcgagacggc aagtggatct
     7141 gtttctactc tgaccggacc ggggagcctc aggtgtggaa agtgcgtgcg gagggaggtg
     7201 gcgaggttca ggtcacgagg aacggc
//
LOCUS       DATIFQ010000524         3523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1713280, whole genome shotgun sequence.
ACCESSION   DATIFQ010000524 DATIFQ010000000
VERSION     DATIFQ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713280"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="VLE22_26400"
     CDS             complement(<1..1668)
                     /locus_tag="VLE22_26400"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSB18010.1"
                     /translation="MRGKTLSIVLGIFVIAGAAWPKRPITHEDVWLMKRVGAPVVSPD
                     GKWAVFSVREPSYDAQKEVTDLWIVPVDGSQQAKRLTFTKGPEGDVDWSPDSTRIAFS
                     AKREGDEAPQIYIIPITGGEAVRLTALSTGAAGPKWRPDGKALLFESRVYPGANDDAA
                     NRRIAQERKNRKYNARMYERLPFRFWDRWLDDTQLHIFVQALTEGSQPRDLLAGTKFV
                     QQPGFGKIEMEDTLNPAWSPDGDSIVFDCSTDLDRSMDRFTTNHVFRIPSQGGEPEAL
                     TSGEDSYGGAVFRPDGKALYMKHDRAYGSGMKLYTLTRLAMLAWPAAAGAKPRILTEG
                     WDRSVDSFRFAPDNRTVYITAEEHGHDRLFVTPAEGGEVKSHHEVTVGGYSGLSIPSK
                     AAATLLVASWFSTTNPPEVFRIDPARGSRTALTHFNDGRVAEIDWQPVREFWFTSKKG
                     TRIHNMVVLPPAFDESKKYPLMVFMHGGPHNQWKDQFFLRWNFQYIASPGYVVLMTNY
                     TGSSGFGEEFAAAINKDPLQRPAAEINQAADEAIRRFPFIDAKRQVAG"
     gene            1842..2654
                     /locus_tag="VLE22_26405"
     CDS             1842..2654
                     /locus_tag="VLE22_26405"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HSB18011.1"
                     /translation="MAKIYYIGDWAVMLGPLFAETPFNYAWKGTDIFNYGHWLKDAIE
                     SGGEHEVTSVPTWEFYRLPPGRYEEILAEYDAIVFSDVEAKNFQLDPHFFDRSKFGKQ
                     PLTFPDRVRLTVEALRSGTHMMFLGGWLSFNGEMGKGGWGRTQLRQILPVECLELEDL
                     RESTEGFRGRALNPKHPILDGVDLSQVPPVLGYNAVKPRRGCEVLAVWEGTKDPMIAV
                     GKFGNGRVLAYTSDPAPHWGCNFVYSDQYPRFWRNAVKWLLSGPKTKKNRRA"
     gene            2712..>3523
                     /locus_tag="VLE22_26410"
     CDS             2712..>3523
                     /locus_tag="VLE22_26410"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18012.1"
                     /translation="MQLHTLDWIIAFLSVAICFIPALFFGKRSGKNTSEFFASGRAVP
                     WWLAGLSMVATTFASDTPNLVTNFVRTQGVAGNWQWWAFVLTGVATVFFYARLWRRSG
                     VLTDLEFYELRYSGKAASFVRGFRSIYLGFFFNCIIMATVNLAACKIAAILFGLDRWQ
                     TLIAVGLLNVIFAAHSGLWGVLVIDMIQFFIKMSAVIAAAYFAISLPEVGGMSGLVEK
                     LSAKQGPGGLNYLNMLPDFQNGWNLAFAVFIMPVAVQWWAVWYPGAEPGGGS"
BASE COUNT          597 a         1133 c         1053 g          740 t
ORIGIN      
        1 gccggccacc tggcgttttg cgtcgatgaa cgggaaccgg cggatcgcct cgtccgcggc
       61 ctgattgatt tcggcggccg gcctttgcag gggatctttg ttgatggccg cagcgaactc
      121 ctccccgaag ccgctcgacc cagtgtaatt cgtcatcaat acgacatacc cgggcgaggc
      181 gatgtactgg aagttccacc gaaggaagaa ctggtccttc cactggttgt gcgggccgcc
      241 gtgcatgaac accatcagag ggtacttctt cgattcatcg aacgccggcg gcagcacgac
      301 catgttgtgg atccgtgtgc ctttcttgga cgtgaaccag aactcccgca ccggctgcca
      361 gtcgatttcg gccacccgcc cgtcgttgaa atgcgtgagc gccgtccggc ttcccctcgc
      421 tgggtcgatc cgaaacacct ccggagggtt cgtcgtggag aaccaacttg caaccagcaa
      481 ggtcgccgcc gccttggacg gaatcgaaag cccgctgtag ccccccaccg tcacttcgtg
      541 gtgcgacttc acctcgccgc cttcggccgg cgtgacgaac agcctgtcgt gcccgtgctc
      601 ctcggcggta atgtagaccg tcctgttgtc gggcgcaaaa cggaagctgt ccaccgaacg
      661 atcccagcct tcggtcagga tcctgggctt tgcaccggcc gcggccggcc aggcaagcat
      721 agcaagcctc gtgagcgtgt acagtttcat cccgctgccg tacgcccggt cgtgcttcat
      781 gtacagcgcc ttcccgtccg gccggaacac ggcgccgcca taggagtcct cgccgctcgt
      841 gagtgcctcc ggctcgccgc cttgtgacgg gatccggaac acatgattgg tcgtaaagcg
      901 atccatggac ctatccaggt cggtcgagca gtcgaaaacg atcgaatcgc cgtccggcga
      961 ccaagccggg ttcagcgtgt cttccatctc gatcttgccg aatcccggct gttgcacgaa
     1021 cttcgttccg gcgaggaggt cgcgcggctg cgagccttca gtcaacgcct gcacgaagat
     1081 gtggagctgg gtgtcgtcga gccagcggtc ccagaaccgg aacggcagcc gctcgtacat
     1141 ccgtgcgttg tacttacggt tttttcgctc ctgggcgatc ctcctgttcg cggcgtcatc
     1201 gttcgctccc ggatacaccc tgctctcgaa cagaagcgcc ttgccatccg gcctccactt
     1261 tggtccagcc gcacccgtcg agagtgcggt cagccgcacc gcctcgccac cggtgatcgg
     1321 aataatgtag atctgtggcg cttcgtcccc ctcacgtttg gctgagaacg cgatgcgggt
     1381 cgaatccggc gaccagtcga cgtcgccctc gggacctttt gtgaaagtca ggcgtttggc
     1441 ttgctggctc ccgtccaccg gaacgatcca gaggtctgtg acttctttct gcgcgtcgta
     1501 cgaaggctcc cgcacggaga aaacggccca cttgccatca ggactgacaa ccggcgcccc
     1561 gacacgcttc ataagccaca cgtcttcgtg cgtgatcggt cgcttgggcc acgccgcacc
     1621 cgcgatgacg aaaattccga gaacgatgga gagcgtcttt ccgcgcattc gatcattctc
     1681 ccaccgggtg cagcgtcgcc gggccaggtt gttttgtggc gcgggccatc gttttccgtg
     1741 gcctgcggct gtcccggttt tctttcagcc tgtggtgtcg aaccggtgcg gcgggtagtt
     1801 cgtcgccgtc gccgattcgc gatagactct ccctttgcac tatggccaag atctactaca
     1861 tcggtgactg ggcggtgatg ctcggtccgc tgttcgcgga gacgccgttc aactacgcct
     1921 ggaagggcac tgacatcttc aattacgggc attggctcaa ggacgccatc gagtccggtg
     1981 gcgagcacga ggtcaccagc gtgccaacct gggaattcta ccggctgcca cccggacgtt
     2041 acgaggagat cctggccgag tacgacgcca tcgtcttctc cgacgttgaa gccaagaact
     2101 tccagctcga tccgcacttc ttcgaccgca gcaagttcgg caagcagccg ctgacattcc
     2161 ccgaccgcgt ccggcttacc gtcgaggcct tgcggtccgg aacgcacatg atgttcctcg
     2221 gcggctggct gagcttcaac ggcgaaatgg gcaaaggcgg ctggggacgg acgcagttgc
     2281 ggcagatcct gccggtggaa tgcctcgaac tggaagacct gcgggagagc accgagggct
     2341 ttcgcgggcg ggccctgaat ccgaagcacc ccatcctcga cggggtcgat ctgtcacagg
     2401 tcccgccggt tctcggctac aacgccgtga aaccccggcg tggctgtgaa gtgctcgcag
     2461 tctgggaagg caccaaggac ccgatgatcg ccgtcggaaa attcggcaac ggccgggtgc
     2521 tcgcctacac ctccgacccc gctccgcatt ggggctgcaa cttcgtctat tcggatcagt
     2581 acccgcgatt ctggcgcaac gcggtgaagt ggttgttgtc cggaccgaaa accaaaaaga
     2641 ataggcgtgc gtaggcgtcc gccccgcccg tcctcgaaat accataagat gttctgttca
     2701 gcgggaacca tatgcagcta cacacacttg actggattat agccttcctg tccgtcgcga
     2761 tctgtttcat ccccgcgctt tttttcggga agcgttccgg gaagaatacc tccgagtttt
     2821 ttgcttcggg gcgtgcggtg ccttggtggc tggccggcct ctccatggtc gccaccacgt
     2881 tcgccagcga cacaccgaac ctggttacga acttcgtccg cacgcagggc gtcgcgggca
     2941 actggcagtg gtgggccttc gtcctcaccg gtgtcgccac cgtgttcttt tatgcccggt
     3001 tatggcgccg gtccggcgtg ctcaccgacc tcgagtttta cgaactgcgc tactccggca
     3061 aggcagcgag cttcgtgcgc ggttttcgtt cgatctatct cggctttttc ttcaactgca
     3121 tcatcatggc tacggtgaac ctggccgcct gcaagatcgc cgccatcctc ttcggtctgg
     3181 atcgctggca gacgctcatc gcggtcggcc tgctcaacgt gatctttgcc gcgcactccg
     3241 gcctgtgggg cgtgctggtc atcgacatga tccagttctt catcaagatg tcggctgtga
     3301 tcgcggcagc atacttcgca atttcgctgc ctgaagtggg cggcatgtcg ggcctggtgg
     3361 agaagctctc cgccaaacag ggaccgggcg ggctgaacta cctgaacatg ctgcccgatt
     3421 tccagaacgg gtggaacctg gccttcgccg tcttcatcat gccggtggcg gtgcaatggt
     3481 gggccgtctg gtatcccggc gccgagcccg gcggcggcag cta
//
LOCUS       DATIFQ010000525        11451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1907367, whole genome shotgun sequence.
ACCESSION   DATIFQ010000525 DATIFQ010000000
VERSION     DATIFQ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907367"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VLE22_26415"
     CDS             <1..362
                     /locus_tag="VLE22_26415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18013.1"
                     /translation="YNQSSPELEEWLPSVVMAFRTTGGILNPLPRPATVKITTGGFGP
                     DNAEKHVDEYVDKLLAQPSGWMVYNLHGLDEEGWGPIRPAYLEKLLGRLREIDSVRIL
                     PTARALMQGKSGSFAGA"
     gene            404..3028
                     /locus_tag="VLE22_26420"
     CDS             404..3028
                     /locus_tag="VLE22_26420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB18014.1"
                     /translation="MKPALAAVLVWGIVWAPAVLTCPAQERQSSRELELLSHVQQRMA
                     EDLDRLPDYFCQETIERSAKLPRQNLLFRDRIRLDVAYIGLKEMFAWPGSAKFEYSSP
                     DRMVSGGASGFGSFGSWTHSLFRSSAPVFGYAGEHIIDGRRTFQYNFQVPLAGSTYKV
                     NVRGREAVVPYSGSIWIDPELLEMLRLEVRAGQSGLPVASVTNVIDYARTLIGSAEFL
                     LPQSAELIMIDAEGNEHRNLTRLTGCRQYTAESSISFDLPAAAPAAVSEARELTLPAG
                     ITLEAKLESPISFEQSAVGDQITARLDRAVNAGSLSLPKGATLSGRIRRLEQYGEPAK
                     LYIVGLEFSSLALANTRVRFRARLTGPLLKVWERKEPAFYGAPPTSRGIESSGLDIDN
                     SDPLSPYGVFRIWGNRLRLSRGLRMIWETQDETAQPALVAKAGADPVPPVAAPQPVQA
                     ARQPAQVAPPPVLPAKPAPAPVIRVTTRLVQVHVLVQDRKGDPISGLTRDDFVLLDEG
                     TPQQITDFSVESGGPSLGPRTPVSPNDPAVRGSRMPIAATAVLFDRLNTSTDDQFYAK
                     RELLKFLRKAKRQERIAIYVLDGNLRVLPDSTDAGELDRALSGRGSAQLDASEPPKPD
                     TGFKAVDKMLSRTNREIASLAVEERVLRTLWALEAVGLQLSRLPGRKNLVWLSGAFPL
                     SIAPELNEMAELRKDHLLFSGQKSFASQARQTTRVLTDANVAVYPVDARGLVGVAEAN
                     AADATPGVLVQGGVEAPQPLPNSAAQSRQATDEQGGVEVPRPLPKSVVQSQQAMERLA
                     RDTGGRAFYGANDLIAGINRAFEDSRLVYVLGFRPSHEEWDGKFHQIKVQVKQPGSQL
                     RHRRGYFAVSESGDRGER"
     gene            3132..3848
                     /locus_tag="VLE22_26425"
     CDS             3132..3848
                     /locus_tag="VLE22_26425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18015.1"
                     /translation="MNGNLGTNLRRIDRRLYVWAAVVASVIAFAGFARTYYLKGAFGT
                     PELPPLVHVHGFVMTLWIATFVLQTTLVAIRRTDLHRRVGVAGALLASLVLVVGLATA
                     INGAKRGSSPGPPPLVFLAVPLADMVVFAVLAGAGLYFRRRSDIHKRLMLLATLSILA
                     AAFARIPLGFIRANPVVMAFGLTDLFVLACVSYDTWARRRLHPAFGWGALLIVASHPL
                     RLMLAKTPAWMQIATWLVRS"
     gene            3947..7102
                     /locus_tag="VLE22_26430"
     CDS             3947..7102
                     /locus_tag="VLE22_26430"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HSB18016.1"
                     /translation="MRIELLGKLRFTFGRRPVTSVSTSRLQSLLAFLVLHAEAAQSRE
                     HLAFLLWPESSESQARTNLRQLLHHLRRALPVECSLLAADNQTVRWRLDSSCSVDVIE
                     FQAAVVRAADAEKKGDSTAAREALEEAARLYQDDLLPDLYDEWLQAKREQLRQQLMDV
                     LSRLATLLEMSGDYPSAIRHATRLVAVDPLREPSYQVLMRLHARNNDRSSALRVYHQC
                     MRTLKRELGVSPSKATQDLFTQALKSEHLPAAPVELPPYAATMPLPMVGRTTEREHLL
                     ACWRRVTQGETHFALILGEPGIGKSRLAEELFQYCSQYPEGAAARARCYFAQGRLAYG
                     PVAEWLRAEPMRFARAQLPKPQLAELARVLPEILVESPAIAPPQPLTESWQRRHLYEA
                     LNAGFSKGAKPLLLLIDDMQWCDHDSFEWLHSFFRSGAAGSTLVLGTVRPEETDRDHP
                     LAGLVGELRQSGQLSEIPLAPLSVDEAAALAVQVAGRECDPAFLSGLYQATKGNPLFV
                     VESVRASLEDQASKSSAPPRVQAMIAARIAQLSLPAYELAELAATVGRPFSFDLLAKA
                     TDWDEESLSRALEELWQRRIIEGQSTGAYDYTHDLLREVVYTELSPIRQRSLHRRVAR
                     ALEELYAPDLEGVSGWLAAHYDAAGMAQQAIRYYRAAASVAKRRFADAEAADLLRRAL
                     QLCRDFPETANRDSHELELLVTLGPSLVTTQGYGMPEVGETYERGLLLSRRSADRKHL
                     FSLLSGAWLFNIVRGQLEESRRLGQDCVDAARREGASALETAGHLLLGISLFHLGQLA
                     ASREQVEQAVPVYGAPSHPALVLFAGPDLGVFCRAYQSHLLWQFGHAGQAAAKSDESI
                     ALARDVSHPFTLAIALDYAAMLNVFRQEGKLALTRAGEASALCRKHGFAYYLAWADIL
                     AGWATAVEGEPAAGLVQLRHGLDALKATGAELRLPFYYGLLAEVCGLAGHVGEALANV
                     ANGFAFQNKNAETWSAPDLHRIQGDLLLRSGDAPEARISYQRAIELAQQTGAQMFGRR
                     AADRLLEVHTVKEVRRKAAER"
     gene            7377..8255
                     /locus_tag="VLE22_26435"
     CDS             7377..8255
                     /locus_tag="VLE22_26435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18017.1"
                     /translation="MTSLWASSFSFTTGIPDGRLGAASRRASPGKLETETADDFFLQQ
                     TTVINGATITGLVALGTPLENIKEVELEVYHVFPLDSAPASGKVPSRVNSPSDVEIDS
                     ATRAGGSGTLTFSASVLNASFSVGNTVVNDIRLPDVPPGGEGPATGEEVEITIVFTSP
                     IILPAGHYFFRPEVLLTDGDFLYLSAPRPIVPPGDPFVGDLQAWIRNSSLAPDWLRIG
                     TDIVGGVPPPTFNMTFSLAGETIPQAGTPGREDCQGRTISALAHQFGGTDAAAFSLGF
                     PSVQALQFGFGAYCRP"
     gene            complement(8382..8795)
                     /locus_tag="VLE22_26440"
     CDS             complement(8382..8795)
                     /locus_tag="VLE22_26440"
                     /inference="COORDINATES: protein motif:HMM:NF021514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HSB18018.1"
                     /translation="MQSVKDLHILLATMKPTLEDGEFVFVSLPAEQQLGPQSDAVIAS
                     FREKEGLTLIVRREESERRGFPSTFPCRMITLNVVSDLEAIGFLARVVTELAARKISV
                     NAVSAYHHDYLFVPTRHAEEALEALRQLQDAHGRH"
     gene            8966..10345
                     /locus_tag="VLE22_26445"
     CDS             8966..10345
                     /locus_tag="VLE22_26445"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSB18019.1"
                     /translation="MKQEAVTQFKSQLRGELIEPTDTRYEEARKVYNAMISRKPRLIV
                     RCADVADVIAAVHFGRQQDLRVSIRGGGHNAGGLGVCDDGLVIDLSLIKYVHVDPAAR
                     TVRVGGGCTWADVDHTTHAFGLAVPSGIISTTGVGGLTLGGGMGHLTRQYGLTIDNLL
                     AADVVLADGSFVVASADENSDLFWALRGGGGNFGVVTSFLFQAHPVHTVCAGPMLWGL
                     DQAADIMKWYREFIVQAPEEINGFFAFLTVPPGPPFPEELHFKKMCGLVWCYNGPLEQ
                     ANEILGPLRSFRRPAFEFFAPMPFPMLQSMFDGLYTPGLQWYWKADFVKELGDEAIAL
                     HVKHGSQLPTLHSTMHLYPINGAAHKAGNGDTPWSFRDAVWTEVIVGVDPDPANKDRI
                     TAWARSYWEALHPYGAGGAYVNFMMEGEGEDRIRATYRNSYEKLARIKAKYDAKNFFR
                     VNQNIRPAA"
     gene            complement(10623..10814)
                     /locus_tag="VLE22_26450"
     CDS             complement(10623..10814)
                     /locus_tag="VLE22_26450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18020.1"
                     /translation="MDGSPVGHDLQRIHKQIHQHTTNATSIQSDDQAFGYSPDDVYGL
                     KFRTDRNLVHGAADQLTNI"
     gene            complement(11068..>11451)
                     /locus_tag="VLE22_26455"
     CDS             complement(11068..>11451)
                     /locus_tag="VLE22_26455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18021.1"
                     /translation="CEQKGRFEEARAALERTLAGSHGHASAPASLGHLFGVTGQREGA
                     LRMRDQLNDLASRGYVAPFWHSILYTGLGDVDAAIGHLERSYAQSDVWLVWLNTDPRL
                     DSLRADARFQRLLRRVGFGAQAAGA"
BASE COUNT         2155 a         3510 c         3540 g         2246 t
ORIGIN      
        1 cgtataacca gtcctcgccg gaactggagg aatggctgcc gtcggtggtc atggcatttc
       61 gcaccacagg cgggatactc aatcctcttc cgaggccggc taccgtgaaa atcaccaccg
      121 gaggcttcgg cccggacaac gccgagaaac acgtggatga gtatgtggac aagctcctgg
      181 cccagccttc tggatggatg gtttacaacc tccacggcct ggacgaggag ggctggggac
      241 cgatccgccc agcctatctc gagaaactcc ttggaaggct ccgggaaatt gattccgtcc
      301 ggatcctgcc gactgccagg gcattgatgc agggaaaaag cgggagcttt gcgggggctt
      361 aatcgtcgcc gagtctgccg tacagcgcta taatcagaac gccatgaaac cggcgcttgc
      421 ggcggttctc gtatggggaa tcgtttgggc gcccgcggtc ctcacctgcc ccgcgcagga
      481 gcgtcaaagc agccgggaac tggagctcct cagccacgtt caacaacgga tggcggaaga
      541 tctcgatcgc ctgccggact atttctgcca ggagacgatc gagcgctcgg cgaagctgcc
      601 ccggcagaac ctgctcttcc gcgacagaat ccggctggac gtggcctaca tcggactcaa
      661 ggagatgttc gcctggcccg gttcggcgaa gtttgagtat tcgtcccctg acaggatggt
      721 ctccggcggc gcttccggat ttggcagctt tggaagttgg acccacagtc tcttccgctc
      781 gtcagcaccg gttttcggct atgccggcga gcacatcatc gacggccgcc ggacgttcca
      841 gtacaacttc caggtacccc tggccggcag cacctacaag gtaaacgtca gaggccgcga
      901 ggccgttgtg ccctactccg gttctatctg gatcgacccc gaattgctgg agatgctgcg
      961 gcttgaggtc cgagcaggcc agagtggcct cccggttgcg tccgtcacca atgtgatcga
     1021 ctacgcgcgc accctcatcg gctcggccga gttcctgctg ccccagtccg ccgagctgat
     1081 catgatcgat gcggagggca acgagcatcg caacctaacc aggctcacag gttgccgcca
     1141 gtacacggcc gagtcgtcaa tctccttcga tctgccagct gccgcgcccg ctgctgtcag
     1201 cgaagcccgg gaactgacac tccctgccgg aatcacgctc gaggcgaaac tggaatctcc
     1261 aattagcttt gagcagtcgg ccgtcggcga ccagatcacc gcccggctgg atcgtgctgt
     1321 caatgcgggc agcctttcgc tgcccaaggg cgcgacgctc tccggccgga tccgccggct
     1381 cgagcagtat ggcgagcccg cgaagctgta catcgtgggt ctggagttct catccttggc
     1441 tctcgccaac acccgtgtgc gtttccgcgc ccgcctgacc ggcccattgt tgaaagtctg
     1501 ggagcgtaag gagccggcct tttacggagc gccgccgacc agcagaggca tcgaatcgag
     1561 cggtctcgac atcgataact ccgacccgct gtccccctat ggcgtgttcc gtatttgggg
     1621 caaccggctg cgcctctccc gcggcttgcg gatgatctgg gagacacaag atgaaacggc
     1681 gcagcccgca ctggttgcga aagccggagc cgatcccgtc ccaccagttg ccgcaccaca
     1741 acccgtgcaa gccgcacgac aacccgcgca agtcgcgcct ccccccgtgc tgcccgccaa
     1801 gcctgcgccg gctcctgtga tccgtgtcac cacccgcctg gtgcaagtcc acgttctcgt
     1861 gcaggaccgg aagggcgacc ccatttctgg cctcacccgg gacgatttcg tgctcctgga
     1921 cgagggaaca ccgcagcaga tcacggactt ctcggtggaa agcggcggtc cttctctggg
     1981 gccacggacg cccgtgtcac cgaacgatcc ggccgtacgc ggctcccgca tgccaatcgc
     2041 cgctacagcg gtgctgtttg acagactgaa cacgagcact gatgaccagt tctacgccaa
     2101 acgagaactc ctcaagttct tgagaaaggc gaagcggcag gaacgcatcg cgatttacgt
     2161 cctcgacgga aacctgcgcg tgttgcctga ttccactgac gccggggaac tggacagggc
     2221 gctgagcgga cgcggttccg ctcaactcga cgcttccgaa cccccgaagc cggacaccgg
     2281 cttcaaggcg gtggacaaaa tgctttcacg aacgaaccgc gagattgcga gcctggcagt
     2341 cgaagagcgg gtactgcgaa cactctgggc cctggaggcg gtcggccttc aactttcccg
     2401 tctgccgggg cgaaagaacc tcgtctggct ttcgggcgcc tttccgctga gtatcgcgcc
     2461 cgagctgaac gagatggccg aactaaggaa ggatcatctc cttttttcag ggcagaaatc
     2521 cttcgcatcc caagccaggc agaccacgcg cgtcctgacc gacgcaaacg tagccgtcta
     2581 tcctgtcgat gcccgcggac tggtgggagt cgccgaggcg aatgcagcag atgccacgcc
     2641 cggcgtcttg gttcaggggg gcgtcgaagc cccgcagccg ttgcccaact ccgcagctca
     2701 gtcgcggcag gcgacggacg aacagggggg cgtcgaagtc ccgcggccgt tgcccaagtc
     2761 tgtggttcag tcgcagcagg cgatggagag actggcgcgt gacaccggcg ggcgggcgtt
     2821 ctacggcgcc aacgacctca tcgccggcat caatcgggct ttcgaggact cacgcctggt
     2881 gtacgtgctc gggtttcggc cgtcgcacga ggaatgggac ggcaagttcc accagatcaa
     2941 ggtccaggtg aaacaacccg gctcgcaatt gcgccaccgt cgcggctact tcgccgtctc
     3001 ggagagcggc gaccgagggg agcggtagcc cgtaccgctt tgacagaaga tacatcctcc
     3061 agttacactc accagttatt caggtgcaca ggcttcggcc tgtgtcagcg ccctgggagg
     3121 ggagctcgta aatgaacggg aatctgggga caaaccttcg ccggattgat cgccggctct
     3181 acgtctgggc ggctgtcgtt gcctctgtga tcgcgttcgc gggctttgcg cggacctact
     3241 acctcaaggg cgcattcggg acgccggaac tcccgcctct agttcacgtc cacggttttg
     3301 tgatgacgct ctggatcgcg acgttcgtct tgcagaccac gctggtcgcg atccgacgca
     3361 ctgaccttca tcggcgtgtg ggcgtcgctg gagccttgct ggcatcactc gttctggtcg
     3421 tcggcctcgc aaccgcaatc aacggagcga agcggggttc atcgcccgga ccgccgccgc
     3481 tggtctttct cgcggttcca ttggctgaca tggtggtgtt cgccgtgttg gcgggagctg
     3541 ggctgtattt ccgccgccgg agcgatatcc ataaacggct gatgctgctg gcgacgctaa
     3601 gcattctggc agccgctttt gcccgaattc cgttgggatt tatccgagcc aatcctgtgg
     3661 tcatggcgtt cggcttgacc gacctgtttg tgcttgcctg cgtgagctat gacacctggg
     3721 cgcgccgccg cttgcacccg gccttcggtt ggggcgcgct actcattgtg gcttcccatc
     3781 ccttgcgctt gatgctggcg aaaacaccgg cctggatgca gatcgcaacc tggctggtga
     3841 gatcgtagca gctcttttcc agcatcttga gttctcaggt gggcgctgcg gctcgctctg
     3901 cgttcaacga tccgcgttct tcggtaccat aacagtagtg cccccggtgc gcattgagct
     3961 tctgggcaag ctgcgcttca ctttcgggcg gcgacctgtc acgtcggtaa gcacaagccg
     4021 tctgcaatcg ctgctggcgt ttctggtgct gcacgccgaa gccgcgcagt cccgcgaaca
     4081 cctcgcgttt cttctgtggc cggagtcgag tgagtcacaa gcccgcacga acctgcgcca
     4141 actgctccac cacctgcgcc gcgcgctgcc agtcgagtgt tcgctgcttg cggccgacaa
     4201 ccagacggtg cgctggcggc tcgacagctc ttgttccgtc gatgtgatcg aattccaggc
     4261 agcggtggtt cgagctgcgg atgccgaaaa gaagggtgac tccacggccg cccgtgaagc
     4321 actcgaggag gcggcgcgac tgtatcaaga cgacctgctg ccggacttgt acgacgagtg
     4381 gctgcaggct aagcgcgagc aattgcggca gcagctcatg gacgttctca gccgtttggc
     4441 gacgctgctc gagatgagcg gcgactatcc gtccgcgatc cgtcatgcca ccaggttggt
     4501 cgctgtcgac ccgttgcgtg agccatcgta tcaagtactc atgcggctgc atgcccgaaa
     4561 caacgaccgg tcgagcgctt tgcgcgtata tcaccagtgc atgcgtaccc tcaagcgcga
     4621 gctcggtgtc agcccgtcga aggcgacgca ggatctcttt acccaggcgc tgaaatcgga
     4681 acatctgcct gctgctccgg tggaactgcc accctacgcc gcgacaatgc ctctaccgat
     4741 ggtgggcagg acaacggaac gggagcacct gctcgcctgc tggcggcgag tgacgcaggg
     4801 cgagacgcat tttgccctga tcctcggcga gccggggatc ggaaagtccc ggctggcgga
     4861 agagctgttt cagtactgct cgcagtaccc ggaaggcgcg gcagctcggg ctcgctgcta
     4921 cttcgcgcag ggcaggctcg cctatgggcc cgtggctgag tggctgcgag cggagccgat
     4981 gcggttcgcg cgagcgcagt tacccaagcc gcaactcgct gagttggcgc gagtgctgcc
     5041 cgagatcctg gttgaaagtc cggcgattgc tcctccacag ccactgacgg agagttggca
     5101 aaggcgtcac ttatacgaag ctctgaacgc cggattcagc aagggcgcga aaccgctgtt
     5161 actgctgatc gacgatatgc agtggtgcga tcacgattca ttcgagtggc tgcactcctt
     5221 ctttcggtcc ggggccgccg gcagcacctt ggtgctcggc acggtgaggc cggaagagac
     5281 ggaccgcgat caccccttgg ccggcctcgt aggcgaattg cgccaatccg ggcaattgtc
     5341 agagatccct cttgcgccgc tgagcgtgga cgaggcggcc gcgctggcgg tccaggttgc
     5401 cgggcgggag tgcgatcccg cgttcctcag cggcctctat caagcgacca aaggaaaccc
     5461 tctcttcgta gtagagagcg tgcgtgcctc actcgaggat caagcatcaa agagctcggc
     5521 gcctccgcgt gtgcaagcca tgatcgcggc gcgtatcgcg caactgtcgc tgccggctta
     5581 cgagttggcg gagctcgcag ccaccgttgg caggccgttc tcctttgatc tgctcgctaa
     5641 agccaccgac tgggacgagg agagcctgtc tcgcgccttg gaagaattgt ggcagcgccg
     5701 catcatcgag gggcagagca caggggccta cgattacact cacgacctcc tgcgcgaggt
     5761 ggtttacacg gaattgagtc ccatacgcca gaggtctttg caccggcggg tagcgcgcgc
     5821 gctggaagaa ctgtacgcgc ccgatctcga aggcgtcagc ggatggctgg ccgcccatta
     5881 cgatgcggcc ggtatggcgc aacaagcgat tcgctattat cgcgccgctg cttccgtagc
     5941 gaagcgacga ttcgcggacg cggaagcggc ggacctgctc cggcgcgctc tccagctctg
     6001 ccgcgacttt ccggaaaccg cgaaccgcga tagtcacgaa ctcgagctgc tcgttacgct
     6061 cggtccctcg ctcgttacca cgcagggcta tggcatgccg gaagttggag aaacgtacga
     6121 gcgcgggctg ctgctttcga ggcggtccgc tgaccgaaaa catttattct cgctgctgag
     6181 cggagcctgg ctttttaata tcgtgcgcgg ccaacttgag gagtcgaggc gactgggaca
     6241 ggattgtgtc gatgcagcgc gccgtgaagg cgcttcggca ctcgagacag ccggccactt
     6301 actcctgggg atcagcctgt ttcatctggg acaactcgcg gcctcacggg aacaagtgga
     6361 gcaggcggtg ccggtctacg gcgctccatc ccatcccgcc ttggtgctct ttgccggccc
     6421 cgatctcggt gtcttctgcc gcgcttatca atcgcatctg ctctggcagt ttggccatgc
     6481 cgggcaagcc gctgcaaaga gcgatgaatc catcgctctc gcccgcgacg tgtcgcatcc
     6541 tttcactctc gccatcgcgc tggattatgc cgccatgcta aacgttttcc gccaggaggg
     6601 caaactcgcc ctcactcggg ccggggaagc gtccgctcta tgccggaagc acgggttcgc
     6661 atattatctg gcctgggcgg acatattagc gggctgggcg accgccgtag aaggggaacc
     6721 ggccgccggg ttggtgcagc tccgccacgg gctcgatgcg ttgaaggcca ccggagcgga
     6781 acttcgcctg cctttctact acgggctgtt ggcggaggtt tgtggcctgg ccggtcacgt
     6841 gggcgaagct ttggcgaacg tcgccaacgg atttgcgttc cagaacaaga acgctgaaac
     6901 gtggtcggcg cccgatctgc accgcattca aggcgatctg ctgctgcgca gcggagacgc
     6961 tcccgaggct cggatcagct accagcgcgc gattgaattg gcccaacaga cgggtgcgca
     7021 aatgttcggg cggcgagcag ccgatcgcct gctggaagtg cataccgtta aagaagtccg
     7081 ccgaaaagcc gccgaacgct gaccctcgtc actctagaac gctttcggaa cgcctcccct
     7141 gtcacattag atatagaact ggatatttcg acctgagcag acaccacttg tcctcgcagt
     7201 tcagtatccg ggcgataact tcagctccgg aaccacttaa gtccatcaca catgagaaag
     7261 gacagaagat gaacagacat aagcctatta caggcaaatg gagactaacg atgagaagag
     7321 ttgtaaattc gcttgtaaat tcaaccggag cgagattggc tttgggtatg tgtgtagtga
     7381 cgtctctatg ggcgtcatcc ttttcgttta caaccggcat tcccgacggc cggctagggg
     7441 cagcctcccg gcgtgccagc cctgggaaac tcgagacgga gacggctgat gacttctttc
     7501 tgcaacagac cacggtcatc aacggggcca caattaccgg gttggtcgcc ttgggaacgc
     7561 ccctggagaa catcaaggag gtggagctgg aggtatacca cgtctttccc ctagactcgg
     7621 ccccagcctc aggcaaggtg ccctcgcggg tcaactcgcc ctcggatgtg gaaatcgact
     7681 ccgccacgcg cgccggaggt tctggaacgc tcacattctc cgcaagcgtg ctgaatgcga
     7741 gcttctccgt gggcaacacc gtagtgaacg acatcaggct tcctgatgtt cctcccggcg
     7801 gcgaaggtcc agcgacaggc gaggaggtgg agatcactat tgtgttcacg agccctatca
     7861 ttctgccagc aggtcactat ttcttccgcc cggaggttct gctgaccgat ggcgatttcc
     7921 tgtacctgtc ggcgccaaga ccgattgtgc ctcccggaga tccgttcgtg ggggacttgc
     7981 aggcctggat ccgtaactcc agcctggcgc cggattggct gcgcatcggc accgacattg
     8041 ttggtggagt cccaccaccc acgttcaata tgacgttttc cctcgctggt gagactattc
     8101 cccaagccgg gacacccggc cgggaggatt gtcagggcag gaccatctct gcgctagccc
     8161 accagtttgg cggcactgat gcagcggcct tcagtcttgg gttcccgagc gtccaggcgc
     8221 ttcaatttgg attcggcgcg tattgcaggc catagccacg gaggatggtg acgccaggga
     8281 tgagtccggt cgcgcaggcc tcccgggcct gcgcaacgga tcccgtaagg tccgggtggc
     8341 gtcgctgtaa caaggcgacg ttgtccgccg ggttgagagg ttcagtggcg accgtgggcg
     8401 tcctgcaact ggcgaagggc ttctagtgcc tcttcggcgt ggcgtgtcgg tacgaacaaa
     8461 tagtcgtggt gataggccga aaccgcattc acgctaatct ttctagcggc caattcggta
     8521 acgacgcggg ccaggaatcc aatcgcctcc agatcggaaa cgacgttcag cgtgatcatg
     8581 cggcacggaa aggtcgaggg gaagccgcga cgctccgact cttcgcgccg cacaatcaat
     8641 gtgagccctt ccttctcgcg aaatgaggcg ataacggcgt cggactgcgg gccaagttgc
     8701 tgttctgccg gaagagacac gaaaacaaac tctccatcct cgagtgttgg cttcattgtt
     8761 gccagcagaa tgtggagatc cttcacggat tgcacgattc acctcgattc gacgtatcct
     8821 accaccccgc gtgagctgcc caacctcaag agcggccgcc acaacaccgc ggaacgctga
     8881 tcctgcgtct tttagaacgg cttcagaacg cctcccctgc caaagtggat attgaacggg
     8941 atgtttcccg gttacggagg aaaccatgaa acaagaagcc gtcactcaat tcaaaagtca
     9001 gctccggggc gagctaatcg agcccaccga cactcgctac gaggaagcgc gcaaggtcta
     9061 taacgccatg atcagccgca aaccgcgttt gattgtcagg tgcgctgacg tggccgatgt
     9121 gattgccgcc gttcatttcg gccggcaaca ggatctgcgg gtttcgattc ggggcggggg
     9181 ccataacgcc ggaggcttgg gagtctgtga cgatggcctg gtcatcgatc tctcccttat
     9241 caagtacgtt catgtcgatc cagcggcaag gactgtgcgt gtcggcggcg gttgcacctg
     9301 ggccgatgtt gaccatacaa cgcatgcatt tgggctcgcc gtcccgtctg gaatcatctc
     9361 gacaaccggc gtcggaggct tgacactcgg cggcggtatg ggacacctga cgcgacagta
     9421 cggcttgacc atcgataatc tgctggcggc cgacgtcgtc ctggccgatg gaagctttgt
     9481 agtggccagc gccgacgaga acagcgacct gttctgggcc ctgcgaggcg gcggcggaaa
     9541 tttcggcgtg gttacgtcgt ttctcttcca ggcgcatccc gtgcatacgg tgtgtgcggg
     9601 gccgatgtta tggggtttgg atcaggccgc cgatatcatg aagtggtatc gcgagttcat
     9661 tgtgcaagct ccggaagaga tcaacggctt ctttgcattt ctcacggtgc cgcccgggcc
     9721 gccgttcccg gaagagttgc acttcaagaa gatgtgcggc ctcgtctggt gctacaacgg
     9781 accgctggag caggcaaatg agattctggg tccgctgcgg agtttccgga ggccggcctt
     9841 cgaattcttt gctcccatgc ctttcccgat gctgcaaagt atgttcgacg gcctttacac
     9901 gcccggcctg caatggtact ggaaggcgga cttcgtgaaa gagctgggcg acgaagcgat
     9961 cgctttgcac gtcaagcacg gctcgcagtt gcccacgctg cactccacga tgcatctgta
    10021 tcccatcaac ggagccgcgc acaaggcggg aaacggcgac acaccttgga gctttcgcga
    10081 cgctgtctgg accgaagtga tcgtcggcgt ggacccggat cctgccaaca aggaccgcat
    10141 taccgcttgg gccagaagct actgggaagc gctgcatccc tatggggccg gtggcgcgta
    10201 cgtgaacttc atgatggagg gcgaaggcga agatcgcatt cgtgcgacgt accgcaatag
    10261 ttacgaaaaa ctggcccgaa tcaaggcgaa atacgacgcg aagaatttct tccgtgtgaa
    10321 ccagaatatt cggccggccg cttaaagcgg agtttgtcag aaaaggaaga gacaaagtga
    10381 acgtagtgac catcaccccc gaacagacag gtccgctatc cgcagcggcg tctatcgtcc
    10441 atgacctgcg caatccgctg gccgcgattc acggcagcgc ggagatgctc gttcactcaa
    10501 ggctctccca accgcaagtc cgccgtatcg cgcgcaatat gtattgcgcc tcggtccgcg
    10561 tgcgcgaatt gctggaggag ttcttagacc ggagtaaagg tgcggagaag aaaatggaat
    10621 tctcagatgt tggtgagttg atccgcagcg ccgtggacaa ggttgcggtc agtgcggaat
    10681 ttcaatccgt acacatcgtc cgggctgtac ccgaaggcct ggtcatcgct ctggatcgac
    10741 gtcgcattcg tcgtgtgctg gtgaatttgc ttgtgaatgc gctggaggtc atgcccaaca
    10801 ggggaaccat ccacatatcg gcggtttccg acagccgttc ggtattcatc cgggttcgcg
    10861 ataccggacc gggaatccct cccgaaattc ggggccggct cttccagccg ttcgcaaccg
    10921 cgggcaaagc gagtggcatc ggcctgggcc ttgcttcttc aaggcaggcg gtgatcgatc
    10981 tcggcggcga gatgtgggcg gagccgtccc cccaaggagc ctgttttgcg gttcgtttgg
    11041 ccagacggcg cggggcagaa gacggactca ggcccccgca gcctgggcgc cgaagcccac
    11101 gcgtcgaagg agtcgctgaa atcgcgcgtc ggcccgcagg ctgtccaacc tgggatccgt
    11161 gttgagccac accagccaca catcgctctg ggcgtagctt cgttccagat ggccgattgc
    11221 ggcgtccacg tcgccgagac cggtgtagag aatcgaatgc cagaacgggg ccacatatcc
    11281 acgcgaggca aggtcgttca attgatctcg catgcggagc gctccctccc tctggccggt
    11341 gacgccgaag aggtgcccga ggctggccgg tgcggacgca tggccgtggg agcccgcgag
    11401 agtccgttca agcgcggcgc gcgcctcctc gaatctcccc ttctgctcgc a
//
LOCUS       DATIFQ010000526        23004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_949540, whole genome shotgun sequence.
ACCESSION   DATIFQ010000526 DATIFQ010000000
VERSION     DATIFQ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 23004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23004
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949540"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1510
                     /locus_tag="VLE22_26460"
     CDS             56..1510
                     /locus_tag="VLE22_26460"
                     /inference="COORDINATES: protein motif:HMM:NF024269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB18022.1"
                     /translation="MRKSVGFVLTALLLGGVACRKAAESPKKIPVTTTSTEARAAFLK
                     GRDLILKLRSAEALPLLDEAISKDPNFALAHLLRAQTATSTREFFDHLKAAVAVAGSA
                     SEGEQWWIRGVEAGANGNTKERGDYYNRLVKAFPDDEDAHQLLGIHYYALQEYDPAIA
                     EFQRAVQLAPNYAPSYNQMGYALRDAGKYQEAEAAFRKYVELIPNEPNPHDSLAELLM
                     KMGRFDESIESYRKALSFNEQFLTAFEGIAANLMYQSKHKEALEQLQKEFDLARNDGE
                     RRRALVSMAVCNADEGKPSEALAKLSKAAAIAEQQGDKAGMGFHTVARADLLLNAGKT
                     REAKAAYDKALELVRQSTVPDTVKRNFELAYHGKLALVAGANKDFENAKKEAETMRAG
                     VEALGNPNQMKAAHEVLGIVSLQQKSYDEAISHLNQADLESPYTMFQLARAYAGKKDA
                     DLAKEWYRKTANAYILPDLNYAMVRKTALNASAK"
     gene            1629..3755
                     /locus_tag="VLE22_26465"
     CDS             1629..3755
                     /locus_tag="VLE22_26465"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HSB18023.1"
                     /translation="MKNILTVAVCVLSVAVVLTAEPAKRSIPKTGYRDWKVYGGGPES
                     IRYSTLDQINRTNVHKLAVAWTYDTEDAFKDSEMQCNPIIVNGVLYATSPKLRVFALD
                     AATGKLIWSFTPPNEGRGIGKLRNRGVTWWDDGKGGRIYVVARGLLYALSARTGKPVP
                     SFGTEGVVDLREGLGRDPAKLSLSTNTPGIVYKDLLILGSIMSEELPSAPGDVRAYDL
                     RTGKIRWAFHTIPHPGEYGYDTWPKDGWQYLGAANCWAGMSLDEKRGIVFAPTGSAAF
                     DFYGANRVGDNLFANTLLALKADTGERIWHFQAVKHDVWDRDFPAPPNLVTVRRKGRL
                     IDAVAQITKSGYVFVFDRENGRPLFPIEYRKVPPSDVDGEVLSATQALPLKPPPFARQ
                     RFTEDMVTRRTPEAHKAVLERLRQVRNGPYFEPPSLQGTVVFPGFDGGGEWGGAGFDP
                     ETGFLYVNSNEMAWILRLVERPKSEGRQTGRSLYLSECAGCHREDLKGTPPEFPSLVD
                     IAKRRTEEEIEGITRKGSGRMPAFSNLTDNAVEAITAFLAGRDAEVNGTPAAPSPMDL
                     KYTTDGYNKFLDPDGYPAFEPPWGTLNAIDLDSGEIAWKIPFGEFPELAAKGMKNTGT
                     ENYGGPVITAGGLLFIGATNHDRKFRAFDKLNGKLLWETMLPASGNATPATYEVNGRQ
                     FVVIGAGGGKSGAPSGGSVVAFALPE"
     gene            complement(3852..4838)
                     /locus_tag="VLE22_26470"
     CDS             complement(3852..4838)
                     /locus_tag="VLE22_26470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSB18024.1"
                     /translation="MTKRTLGKSGLTVSAMGLGCMGMSEFYGPGDDAESIATIHRAVE
                     LGIDFLDTADIYGQNRNEELVGRAIRGRRHEVIVATKFGIVRGPDPKSRSVNGRPEYV
                     KASCEGSLKRLGIETIDLYYQHRVDPNVPIEDTIGAMADLVRDGKVRFLGMSEASPQT
                     IRRAFAVHPITALQTEYSLWSRDPEDELLPTVRELGIGFVAYSPLGRGLLTGQIRRFE
                     DLADDDFRRLSPRFNGENFMKNLEVVDRIHDMARQKGCTSAQLSLAWVMAQGSDIVPI
                     PGTKRRRYLDENAAAAGIVLTAGDLARIEQVSPRGAVAGERYHEGAMRFIDR"
     gene            complement(4848..8027)
                     /locus_tag="VLE22_26475"
     CDS             complement(4848..8027)
                     /locus_tag="VLE22_26475"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HSB18025.1"
                     /translation="MKVTALLTSFLAFALLGQQRPEWDDPVVLHVNKERPHTTMKVYT
                     SADLRFAWHTLLNGNWKFHLSPTPAGRPPDFYRPDFDDSGWKTIPVPSSWQMHTDDIP
                     IYTNIIYPFPMSPEEPPLVPKENNPVGSYRTAFEVPAEWKGRQLFITFDGVDSAFYLW
                     VNGQKVGYSEDSRTPAEFNITRYAKPGKNLLAVEVYRWSDGSYLEDQDMWRMSGIFRN
                     VHLWSTGEQHIRDFEIRTDLDAAYRDAVLLVRADVYNFTLSQANVSVTLEMLNDDNTP
                     VFPPQTRRVTPNMRGETSAEFSVPVKNPRKWTAETPNLYRVLLTLKNAAGDTLEVVPS
                     HTGFRKVEIRNGRLLVNGQAILLKGVNRHEHSPDTAKYVPRELMEKDVVLMKQFNINA
                     VRTSHYPNDPEWYDLCDRYGLYVIDEGNIEAHGYGMSKSNYLSNSPDWRGAYLDRVQR
                     MIERDKNHPAVIIWSLGNESGDGPNVEIVYKWVKRRDPSRPFHYEGSTGLGGSNADIN
                     SFMYPPPDVMVQHAKEKPHMPLILCEYTHAMGNSNGGLKEYWDIFYSGTNAQGAFVWD
                     WVDQGIRQPVPEKFRTTSGKDTFLAYGGWWENRAGVRNDDNFCQNGLVGADRNPHPGL
                     WALKYVHRFIHAAASDLTAGKVRVKSWFDFVNAKDVAEGKWEVKADGETIASGAMPEL
                     DLLPRAEKEYTLPLPRIRPEPGVEYWLNLSFVTKNDTSWAKKGHEASWEQFRLPASAP
                     AKAADAARLPPLGMSEVGSQVRFQGPDFGLVFDKKTGVISSMTYKGTPLIERGPVPDF
                     WRMPTDNDRGAWKSLSPQQQGDPANNLAVWRNAGAAWRVEDVQVQRLDDRSARIRVRA
                     DLPEVQARYTAVYTVHGSGDVIVEGSYQPGGHTIAMMPRFGMELVASPGLEQVTWYGR
                     GPSPTYRDRAFERVNVYTSTVDKEWTEFSRPQENGNKADVRWIALTNSNGIGLLATGM
                     PLLSTGVVHYTKKEIEDADYSFKLKRHPEIYLNLDHQQMGVGGIDSWSLNAYPLTPYR
                     LPGDRPYSYKYRLCPVDGNAAAKARELWQ"
     gene            8133..9038
                     /locus_tag="VLE22_26480"
     CDS             8133..9038
                     /locus_tag="VLE22_26480"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB18026.1"
                     /translation="MSATRRQFLSTSMGAAAAAALPPGKDDISLANWSIVQSFRAGKW
                     KLLDMPRILRDVLKINGLEYVNTFFENPTIEYLRKLKRNCEDYGMTSVLVMVDDEGNT
                     ASLDKAERMQAAVAHRKWIDVAHYLGCHAIRCNLRGGPKEWQQDKDVAKRGAEVFRDI
                     LEYAKGSGLNVLIENHGGASSDPDVLLELMRLVNHPKFGMLVDTGNWNPGVDRYEATR
                     KLIRYGKGMSVKGTWGPNKRADFDMEKLLQVCIDGGYHGWWGIEVGWRRPEGVQATPD
                     EIWENEIKSVLAAKTVVERVVLKKA"
     gene            complement(9052..12219)
                     /locus_tag="VLE22_26485"
     CDS             complement(9052..12219)
                     /locus_tag="VLE22_26485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18027.1"
                     /translation="MTPAPLTRKFNLDTSTLVPGSAASIEISGTTAADVLQAVLANSP
                     FPDRRIDLGGVAFEAEAGRDIRFNAAKGAVAFRASGGAHARLAVLNTGEEVRGALPLA
                     DAGEIGLAFTDQQDMRYLLFVAGYEVEGNVDGSHPIGMLGSVTFGVEARREQLYAVIH
                     RFPRDLGANTAIRAMVSSFRPPRHVNSESDLQPGTMLVAEVEGSVAVNVAAKLGYDFT
                     FVREASALGLTGDIGLKLDAGLQATIGLEASGRYLVTLDRESVDANSAEVRLRLFKLS
                     KKGWNFGLNLSASVQGVADGLPENVDGLVKAVFGIHGQQVVKDLLLIEKWTDPNRNLS
                     DTVAGLARDTGLDLLKTTTGVDPQQNFNQARTVLLKALKQWDSLPARVSGTLWALLES
                     GGLQGQTRKTLVASLSLLAGTREDARKKELRRLLQSVGIEDTPSGRLLAAVADRGLLD
                     LLGRSEEVRSVASNILQVLDGGVIEKLQQFIEEKLNIDQVRNVITRADFNAIEGWLVN
                     RLSTFLDRKLRFEDLDEIKNAINAVIRKRQEIYEQARNALARRYDFSFAYAYQKATQR
                     TALLDAKFDLKKPKAAELMKAVLQESRLDVLLVNQVDGVTLQEGVLTHEITRRGSVEV
                     NMPYYNFRSDKLNQSLASVRAVEDRGRVLFYELDTKDDVSIRNRMRSQLALAAAVPVQ
                     TAGALRIYSEVTAQWAYQYRMARENMRRVELERQLGPFVDRYFPEQFAQGGPSSFSTW
                     LTDLDRCVEDVLGRPTDDFGDVLLALEVTVPAKAIRSWLAPRNAEDVRAASMAVSRAL
                     QAGLKELVPFHYFQDVNRLRQNMPAAALLLYAAIPPSTNIRVSGRATGLNADLIDKKE
                     KKVHWDWRDPDKRRRMVFHERTLQRLAVLIAGARERLLAAGQASEAAFFAPDELGDFI
                     ASAAWEELDGHLGALLRLEDIIANGAAASLKEIQGFREQQAAVPSKAIERLAEFGAEI
                     TRTYHERLSSRYGTEALRPLGSMLYLEAARTLDPELASTRPSAMLSLTVLKEERTFAL
                     PKFLDGETPKKEDIAVAQRLVSSTA"
     gene            13192..13935
                     /locus_tag="VLE22_26490"
     CDS             13192..13935
                     /locus_tag="VLE22_26490"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB18028.1"
                     /translation="MPPDLTYRDTPDGSPHHIYDDVLVAIDGSRGINNGQPSALARWL
                     DTLDLTPGERFLHIGCGVGYYTAIAAEALSQSGKVFGIEIDAELAERASRNLSGYANI
                     TVVCGDGSRLAGDSYDAILVNAGATEVQEGWIDQLRPGGRLLVPLTTPIPGMDVGVGH
                     MLLITRHPRGYAAGFVSPVGIFHCAGARTGEGEELLKQSYAGGGEDTVRSLLREEHHP
                     SAQCWLHRPGFCLSRLTIEGCFSTSTRPA"
     gene            complement(14048..17587)
                     /gene="metH"
                     /locus_tag="VLE22_26495"
     CDS             complement(14048..17587)
                     /gene="metH"
                     /locus_tag="VLE22_26495"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HSB18029.1"
                     /translation="MTERADRRGALLDALERRILVLDGAMGTMLQQAHLSAADFGGAS
                     LEGCNEILVQTRPDVVLGVHRAYFEAGADIVETDTFGGTKLVLAEYGLGDRAYELNRT
                     AAMLARQAAAEFSTSGMPRFVAGSLGPTTKAITVTGGVTFRELRDVYYEQAKGLIDGG
                     ADLLLLETCQDTRNVKAALLAFRQLDCESGFSTPVMVSGTIEPTGTMLAGQTADAFWA
                     SVSHMDLLSIGLNCATGPEFMTDHIRTLHEMAWTRISCYPNAGLPNEEGKYGETPESL
                     AAQLERFADHGWLNLVGGCCGTTPAHIRMLAQMVEGRRPRTVPGRPGRAFYSGIELVE
                     ADDSNRPLIVGERTNVIGSRAFKEMIAGENWEEASEIARRQVRNGAHIVDVCLQSSER
                     EEIRDIPPFYDRLIRKIKVPVMIDTTDPAAVELALSYCQGKSIINSVNLEDGEEKFER
                     VCPIARAYGAALIVGTIDEDPVQAQAFTRERKLAVAERSVRLLTEKYGIPPEDLILDP
                     LVFPCATGDENYVGGAVETIEAVRLIKEKIPYVKTILGVSNVSFGLPAAAREVVNSVF
                     LYYTTKAGLDLAIVNSEKLERFASIPEQERRLAEHLLFNTPPADAPTEDLRLASEDWR
                     EQTREQKIAINQHHIAAISERFRGRAASKKKELSLLPLDQRLANYIIEGSKDGLIADL
                     ELKRAVGAAPLDIINGPLMAGMTEVGRLFNNNELIVAEVLQSAEAMKAAVTHLEQFME
                     RADSATRGKVLLATVKGDVHDIGKNLVDIILSNNGYKVINLGIKVPPEELIRAYHEHH
                     PDAIGLSGLLVKSAQQMVVTAEDLRDAGIRVPVLVGGAALSEKFTRLKIAPAFGEAVC
                     YAKDAMSGLALMNQIVDPAARDEVLRAHTHQEALAEEQAGVKAAPVGEQRSSKVRTGV
                     PLPDAPYLDRKVRDVPQLAEVWSYVNPYMLFGKHLGFKGNFEKRLAERDEKALELYHQ
                     MEEVKRWAAGFMKVRAVWQFFEAERDGNAIQLFAPGARSPLHTFRFGRQPRADGLCLS
                     DYVLDPEEGRRDHIAILVATAGAGIRERAEEAKAAGEYFRAHALQALALETAEGCAEW
                     LHRRIREDWGFPDPPAMTMQGRFTSRYRGKRYSFGYGACPNLEDQLGIWKLLNPGEIF
                     VHLTDGFMMDPEASVSALVFHHPDCAYFTAEEG"
     gene            complement(17632..19050)
                     /locus_tag="VLE22_26500"
     CDS             complement(17632..19050)
                     /locus_tag="VLE22_26500"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HSB18030.1"
                     /translation="MSLREQLARQQAQIAELQKTLEAQGRALDRLAQQSSSDVVTAAV
                     PAATALKASSASVDDLAKKTEAVSKNLGGFQFSGDFRYRFDVQARSSNEVAAPLQNAR
                     SRYRLRLNIDKDLDPRFRFHVQLSTGPYNNAITNDQDMAGTVAKHPISIAEAYVNFHP
                     NANVSLRGGRMEEVFADNMRFLWDDDVRFNGFHQTFKGSFASNPLGFKTVELRVGEYI
                     LSNPAVYVLPANSPFVAAGYQVGQKVRDANLFHPGVILRGDLGPAWSHQILGTFQIYR
                     NPNQIQLASTAAGFPVVVTKDLGFVLSGPIGGTGNGTTLPGGATYFAPDFHIAHLGYR
                     LERKGVSIGGREMPLWFDLQAARNTGASSLRDALMASVNLGAVRKAGDLRTLYQFAIK
                     DANSMISQFTDDDLGTGSGVNIAVHALRFDLGLTRFLQWQNLLFIQNQRRASNPSEQF
                     FVPLQRGANSTFRYLGQLAFTF"
     gene            complement(19148..20902)
                     /locus_tag="VLE22_26505"
     CDS             complement(19148..20902)
                     /locus_tag="VLE22_26505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB18031.1"
                     /translation="MTLSSPIFRKLLLGAFLLILLTVVVLDLYVTRFVSAREVDAVER
                     QLDSQARLLSGEVQVVPAPELQSWSRQAQQRAQSRITVIDPAGVVIADSERDPARMEN
                     HAGRPEVRQAMEGRRGVSIRHSDTVDVDLVYLAIPLTYGAKPGHVFRVAVPLKNLNAA
                     IADVRWGILRVSLIATLAALAVAYFITRYVTGRIRRVQQFAEGLVQARFTDRMEPEPA
                     DELGSLARSLNGMAEQMQDLIERLSLESARREAMLSSMVEGVLAVDHELCVTFCNESL
                     ARAVGARVPVPDRLPLLELIRDPAFLNLLTRVLVTGASQKERLQLAAAEGRSFEIQAA
                     PLDVRTRRGAIAILHDITDLERLERVRKDFVANVSHELRTPLTAIQGYAETLLEGALE
                     DKEHNRRFLEIIKSHAVRLNTIASDLLVLSELESGEAAPAPERISVRGAIESALRSVE
                     SEAKLRQVQIHCGGLADVAVTGSKIRLEQALVNLLDNAVKFNRPDGEVWVEAGCTAEG
                     NVRLTVADNGIGIPSDDLPRIFERFYRVDKARSRDVGGTGLGLSIVKHIVERMGGTVS
                     VESQLGKGAKFTMLLPPG"
     gene            complement(20899..21588)
                     /gene="phoU"
                     /locus_tag="VLE22_26510"
     CDS             complement(20899..21588)
                     /gene="phoU"
                     /locus_tag="VLE22_26510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HSB18032.1"
                     /translation="MSHFLEELEELIKRLLEMAGLVESAVHESVLSLTERSETRAQRV
                     LLDEARINQMEIEIDDQATGLLARYQPMATDLRLLTSIIKINNDLERMGDLAVNIVER
                     SLSLIRQEPVKPLIDIPQMARLVESMMRRSLDAFVKRDADLARGVLLSDDAVDDLRDN
                     IFQELIGFMQRDPSTITRSLDLILVARNLERIADHATNIAEDVVFFVKGIDVRHHAET
                     RGETPIGGPIR"
     gene            complement(21616..22452)
                     /gene="pstB"
                     /locus_tag="VLE22_26515"
     CDS             complement(21616..22452)
                     /gene="pstB"
                     /locus_tag="VLE22_26515"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HSB18033.1"
                     /translation="MVTHDDSTMMIGPRDQKSQPESTPQRTADFVPKLRVSGLSFYYG
                     PQRALWDIHLEIPEKKVTALIGPSGCGKSTFLRTLNRMYETVRHARAEGEVLLDGENT
                     LTMDVVELRRRVGMLFQKSNPFPKSIFDNVAYGLRINTGISRAAIKDAVEHSLRRAAL
                     WDEVKDQLHKSALALSGGQQQRLCIARALAVDPEVLLLDEPCSALDPIATAKIEELLV
                     RLKENCTIVIVTHNMQQAARVAEYTGFFLLGRLIEFDQTKTIFTNPSRKETEDYITGR
                     FG"
     gene            complement(22455..>23004)
                     /gene="pstA"
                     /locus_tag="VLE22_26520"
     CDS             complement(22455..>23004)
                     /gene="pstA"
                     /locus_tag="VLE22_26520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HSB18034.1"
                     /translation="FPFVIRYVTDLLNGVPSIVIGIFAYAVLVVPMKRFSTLAGALAL
                     SLMVIPITVRSTEQFLRAVPHSLREGALALGATKWWTIATVVIPAGIRGVLTGTILGV
                     ARVAGETAPLLFTSFSNRYWSTSWDQPVASLPVMIFTYAISPYEDWHRQAWAAGLVLL
                     GLVLVANVAARLILSPKSFRSA"
BASE COUNT         4333 a         7339 c         6866 g         4466 t
ORIGIN      
        1 ccgcggagtc cgtggcacac cgatacctgg acagaatcac aatcgagagg gtgtcatgag
       61 aaaaagcgtt gggttcgttc tcaccgctct gttgctcggt ggagtagcct gtcgtaaagc
      121 cgccgagtcc ccgaagaaga ttcccgtcac tacaacctcg acggaagcac gggccgcatt
      181 tctcaagggt cgggacctga tcctgaagct gcgttccgca gaggctctgc cgctgctgga
      241 cgaggcaatc agcaaagacc caaatttcgc gttggcgcac ctgctgcgcg cgcagacagc
      301 aacgtcaacc agggagttct ttgaccatct gaaggccgcg gtagcagtgg ccggcagcgc
      361 ctcggaggga gagcagtggt ggattcgcgg agtggaagcc ggcgctaacg ggaacaccaa
      421 ggagcgcggc gactactaca accgactggt gaaagctttt ccggatgacg aggacgctca
      481 ccagttgctc gggatccact actatgccct ccaggagtat gaccccgcca tcgcggaatt
      541 ccaaagggcc gtgcaattag ccccgaatta cgcgccttcg tacaaccaga tgggctatgc
      601 gcttcgggac gccggcaagt accaggaggc ggaagcagcc ttccgtaagt acgtcgagct
      661 catcccgaat gaacccaacc cgcacgattc gttggccgaa ttactgatga aaatggggcg
      721 attcgacgag tccattgagt cttaccggaa ggcattgtcg ttcaatgaac agttcctcac
      781 cgcttttgag ggaattgcgg cgaacctcat gtatcagagc aagcacaagg aggccctgga
      841 gcaacttcag aaagagttcg acctggctcg caatgacggt gaacggcgcc gggcgctcgt
      901 ctcgatggcg gtctgcaatg cggatgaggg caagccctcc gaggcgctgg cgaagctatc
      961 caaggccgcg gctatcgccg agcaacaggg cgacaaagcg gggatgggtt tccatacggt
     1021 cgcgcgggcc gacttgctac tgaatgcagg gaagaccaga gaagcgaagg cggcgtacga
     1081 caaagctctc gaattggtac ggcaatcgac ggttccggat acggtgaaga ggaacttcga
     1141 gctggcttac cacggcaagc ttgcgttggt cgccggcgca aacaaggatt tcgagaacgc
     1201 gaagaaagaa gccgaaacca tgcgtgcggg cgtcgaagca ctcggcaacc ccaatcagat
     1261 gaaggcggct cacgaggtgc ttgggatcgt atcactccag caaaagagct acgacgaggc
     1321 gatttcgcat ctgaatcagg cggacctcga atcgccctac acgatgttcc agttggcccg
     1381 ggcgtatgca ggcaagaaag acgcagatct ggcgaaagaa tggtaccgga agacggccaa
     1441 cgcttacatc ctgcctgacc tgaattacgc catggtcagg aagacggcat tgaatgcttc
     1501 ggcaaagtag gcggtagacg aacgcccatt gcagtcgccg acccttgtgg gtcttcccgg
     1561 aggaacggaa ttttttcaca atcccccacg cgctccctgt atagtaggaa gaaatcctgg
     1621 acccgccaat gaaaaacatc ttgactgtgg ctgtgtgcgt cttgtccgtc gcagtggtat
     1681 tgaccgcgga accggcgaaa cgatctatcc cgaaaaccgg ataccgcgac tggaaggttt
     1741 atggtggcgg tccggagagc atccgctact ccacgctcga ccagatcaac cgcaccaacg
     1801 tgcacaaact cgcggtcgcg tggacatacg acaccgagga cgccttcaag gattcggaga
     1861 tgcagtgcaa cccgatcatc gtcaacggcg ttctgtatgc tacttcgccg aagcttcgcg
     1921 tcttcgcgct cgatgccgcc accggaaagc tgatctggag tttcaccccg cccaacgaag
     1981 ggcgcggcat cggcaaactg cgcaatcgcg gcgtcacatg gtgggatgac ggaaagggcg
     2041 gccgcatcta cgtcgtcgcg cgtggtttgc tctacgctct gagtgcgcgc accggaaagc
     2101 ccgtgccgtc gttcggtacc gagggcgtcg tggaccttcg agaaggtctg ggccgcgatc
     2161 cagccaagct ctctctttcc actaacacac ccggcatcgt ctacaaggac ctcctcatcc
     2221 tcggcagtat catgagcgag gaactgccct ccgcgcccgg cgacgtccga gcgtacgatc
     2281 tccgcacggg gaagatccgt tgggccttcc acacgattcc gcaccccggg gaatacggtt
     2341 acgacacctg gccgaaggac ggctggcaat acctcggcgc cgcaaactgc tgggccggca
     2401 tgagcctcga cgagaagcgt gggattgtgt tcgcgccgac cggttccgcc gctttcgatt
     2461 tctacggcgc caaccgcgtc ggcgacaatc tcttcgccaa caccctgctt gccttgaagg
     2521 ctgacaccgg cgaacgcatc tggcacttcc aggcggtgaa gcacgatgtc tgggaccgcg
     2581 acttccccgc cccgcctaac ctcgtgacgg tccgccgcaa gggacgcttg atcgacgcgg
     2641 tcgcgcagat cacgaagtcg ggctatgtct tcgtattcga ccgggagaac ggccgcccgc
     2701 tgtttcccat cgagtaccgt aaggtcccac catccgacgt ggacggggag gtgctctcgg
     2761 cgactcaggc gctgccgctc aagccgcctc cattcgcgcg gcagcggttc acggaagaca
     2821 tggtcactcg gcgaacgccc gaggcccaca aagcggtcct cgaacgcctg cggcaggtcc
     2881 gcaacggtcc gtatttcgag ccccccagtc tccaaggcac tgtagtcttt cccggcttcg
     2941 atggcggcgg cgaatggggc ggcgccggtt tcgatccgga aacgggcttc ctctacgtga
     3001 actccaacga aatggcctgg atactccgcc ttgtcgagcg cccgaagagt gaaggccggc
     3061 agacgggccg cagtctctat ctcagcgagt gtgcaggatg ccaccgcgag gacctcaagg
     3121 gaacgccgcc ggaatttccc tcgctcgtag acatcgccaa acgccgcacg gaagaggaga
     3181 tcgaaggcat cacccgcaag ggctccggcc gcatgcccgc gttctccaac ctgacggaca
     3241 acgccgtcga ggccatcacc gccttcctcg ccggtcggga cgccgaggtg aacggcactc
     3301 cggcagcgcc gtcgccgatg gacctgaagt acaccacgga cgggtataac aagtttctcg
     3361 atcctgacgg ttatccggcc ttcgagccgc cctggggcac gctcaacgcc atcgacctgg
     3421 actcggggga gatcgcgtgg aagattccgt tcggcgagtt tccggagctc gcggcaaaag
     3481 gaatgaagaa caccggcacg gagaactacg gcgggccagt gatcaccgca ggcggtctgc
     3541 tctttatcgg cgcgacgaac cacgaccgca aattccgcgc gttcgacaag ctcaacggaa
     3601 agctgctgtg ggaaacgatg ctaccggcgt ccggcaacgc caccccggca acctacgagg
     3661 tgaacggccg acagttcgtg gtgatcgggg cgggcggcgg caagtccggc gccccgtcgg
     3721 gcgggagcgt tgtggcgttc gcattgccgg agtgaggggg cgccgttgta agtagatcgg
     3781 cacaggctga aggccgtgcc gccgaaagtg gcacggcctt taggcggtgc aatgcatttc
     3841 tgcctgttcg gttaccggtc gatgaagcgc atggcgcctt cgtggtagcg ctcgccggcg
     3901 acagcgccgc gcggcgaaac ctgctcgata cgggccaggt cgccggcggt gagcacgata
     3961 cccgccgcag cggcattctc gtcgagatac ctccgcctct tcgtgcccgg aatcggaacg
     4021 atgtcgctcc cctgcgccat aacccaagcc agagaaagtt gcgcggacgt acaacccttt
     4081 tgccgggcca tgtcgtggat ccggtcgacg acttcgagat tcttcatgaa gttttcgccg
     4141 ttgaatcgcg gcgaaagacg ccggaaatca tcatccgcga ggtcttcaaa gcggcgaatc
     4201 tgtccggtga gcaacccgcg gccgagcggg ctgtaagcga cgaagccgat acccagctcg
     4261 cgcaccgtgg gcagcagctc gtcttcgggg tccctgctcc agagggaata ctccgtttgc
     4321 agcgcggtga tcggatgcac cgcaaaggca cgccggatcg tctgtgggga cgcttccgac
     4381 atcccgagga aacgcacctt cccgtcgcgc acaagatcgg ccatcgctcc gatcgtgtcc
     4441 tcgatcggca cgtttgggtc cacgcggtgc tggtagtaga ggtcgatcgt ttcaatgccg
     4501 agtcgcttca ggctcccctc gcaactggcc ttcacgtact ccggccggcc gttgacacta
     4561 cgcgattttg gatcgggacc gcgcacgatc ccgaacttcg tggccacgat cacctcgtgc
     4621 cgccgccctc gaatcgcccg tccgacaagt tcctcattcc ggttctggcc gtagatgtcc
     4681 gcggtgtcca ggaaatcgat ccccagctca accgcgcggt gaatggtcgc aatcgattcc
     4741 gcgtcgtcgc cgggtccgta gaactcggac atgcccatgc acccgagccc catcgcagac
     4801 acagtcaatc cggactttcc aagcgttctt ttcgtcatta ccatctccta ttgccagagt
     4861 tctctcgcct tcgccgcggc attcccgtcc acgggacaca accggtactt atacgagtag
     4921 ggccggtcgc cgggcaacct gtacggtgtc aacggatatg cgttcaggct ccaactgtcg
     4981 atgccgccca cgcccatctg ctggtgatcg agattgaggt agatctccgg atgtcgcttc
     5041 agcttgaacg agtagtcggc atcctcgatc tccttcttcg tgtagtgcac gactcccgta
     5101 ctgaggagcg gcatgcccgt ggccaggagg cctatgccgt tcgagttcgt cagggcgatc
     5161 caccggacat cggctttgtt cccgttctcc tgcgggcggg agaactcggt ccactccttg
     5221 tccactgtcg atgtgtagac gttcacacgc tcaaacgccc ggtcgcggta ggtcggcgac
     5281 ggaccgcgac cgtaccatgt cacctgttcg agtccaggcg acgccaccag ctccatgccg
     5341 aacctcggca tcattgcgat ggtgtgccca cccggctggt aggacccttc gacgatcacg
     5401 tctccgctcc cgtggaccgt gtagaccgcc gtgtacctgg cttggacctc cggcaggtcc
     5461 gcccgcaccc gaatgcgggc cgaccggtcg tccagtctct gcacctgcac gtcctccact
     5521 cgccacgccg caccagcgtt tcgccatacc gcgagattgt tcgccggatc gccttgctgc
     5581 tgcggtgaca ggctcttcca tgccccccgg tcgttatcgg tcggcatgcg ccagaaatca
     5641 gggacgggtc cccgttcgat cagtggcgtg cctttgtacg tcatcgacga gatgacaccc
     5701 gtcttcttgt cgaagacgag cccgaagtcc ggtccctgaa accgcacctg acttcccact
     5761 tccgacatcc cgagaggcgg aagtctcgcc gcatctgccg ctttagcggg cgcggacgct
     5821 ggcagccgga actgctccca cgacgcctca tgccccttct tcgcccaaga cgtgtcgttc
     5881 ttcgttacga aactgagatt cagccagtac tcgacgcccg gctccggcct gatccgcggc
     5941 aggggtagcg tatattcctt ttcggcccgc ggcagcaggt ccagttccgg catcgcccca
     6001 gatgcgatcg tctcaccatc ggccttgact tcccacttgc cctccgcgac atccttcgca
     6061 ttcacgaaat cgaaccagct cttcacccgg accttgcccg cggtcaggtc gctcgcagcg
     6121 gcgtggataa agcggtgaac gtacttcagc gcccacaatc cgggatgtgg attacggtcg
     6181 gcgcccacga gcccgttctg gcagaagttg tcatcgtttc gtacgccggc acggttctcc
     6241 caccagccgc cgtaggcgag aaatgtgtct tttcccgagg ttgtccggaa cttctccggc
     6301 accggctggc ggataccctg gtctacccag tcccacacaa acgcgccttg tgcgttcgtc
     6361 cccgaataga agatgtccca gtactccttg agcccgccgt tgctgttgcc catcgcgtgc
     6421 gtgtactcac acaggatgag cggcatgtgg ggtttctctt tcgcatgctg taccatcaca
     6481 tcgggcggcg gatacatgaa tgagttgatg tccgcgttcg atcctcccag gcccgtgctg
     6541 ccctcgtagt ggaacggccg tgacgggtca cgccgcttca cccacttgta cacgatctcc
     6601 acgttcggcc catcaccaga ctcattcccg agcgaccaga tgatcaccgc gggatggttc
     6661 ttgtcgcgct cgatcattcg ttggacccgg tcgagatacg cgccgcgcca gtccggcgag
     6721 ttgctgagat agttcgactt cgacattccg tagccgtgtg cctcgatgtt gccttcgtcg
     6781 atcacgtaca gaccgtaccg atcgcacagg tcataccatt ccggatcgtt cggatagtgc
     6841 gacgttcgga cggcgttgat gttgaactgc ttcatcagga cgacgtcctt ttccatcagc
     6901 tcccgtggca cgtacttcgc cgtgtccggg ctgtgctcgt ggcggttgac gcctttgagc
     6961 aggatcgcct gcccgttcac gagcagccgt ccgttcctga tctccacctt gcggaagccg
     7021 gtgtgcgacg gaaccacttc gagcgtgtcg ccggcggcgt tcttcagcgt caggagcaca
     7081 cggtacaggt tcggcgtctc cgcagtccac ttccttgggt tcttcaccgg aaccgagaat
     7141 tccgccgacg tctcgccccg catgtttggg gtcacacggc gtgtctgcgg cgggaacaca
     7201 ggcgtattgt cgtcgttgag catctcgaga gtgaccgaga cgttcgcctg gctcagcgta
     7261 aagttgtaga cgtccgcccg gacaagcaac acagcgtccc ggtatgcggc gtcgaggtcg
     7321 gtgcgaatct cgaaatcgcg gatgtgctgt tctcccgtgc tccaaaggtg gacgttccga
     7381 aagatcccgc tcatcctcca catgtcctga tcttccaggt acgacccgtc gctccagcgg
     7441 tagacctcaa ccgccagcag gttcttcccc ggcttcgcgt atcttgtgat gttgaactcc
     7501 gccggggtgc ggctgtcctc gctgtagccc accttttgcc cgttcaccca taggtagaag
     7561 gcagaatcga cgccgtcgaa agtgatgaag agttggcgcc ccttccactc cgcgggaacc
     7621 tcgaaggcag tgcggtacga gccgaccggg ttgttttcct tcggtaccag cggcggctcc
     7681 tccgggctca ttggaaacgg atagatgatg ttcgtgtaga tcgggatgtc gtcagtgtgc
     7741 atttgccagc tcgacggcac ggggatcgtc ttccagccgg agtcgtcgaa gtccggccgg
     7801 tagaaatccg gcgggcggcc ggctggggtg ggcgacagat gaaacttcca attgccgttg
     7861 agcagcgtat gccacgcaaa ccgcaggtcc gccgaagtgt agaccttcat ggtcgtgtgc
     7921 ggccgctctt tattgacgtg cagcaccacc ggatcatccc actccggccg ctgttggccg
     7981 agaagggcga acgcaagaaa tgaggtaagg agggccgtga ctttcacagt ccccttttac
     8041 cactcgtgga gcgacgcgcc caagtgcctc ggcagcctgc atttccccgc gacccacaag
     8101 ctgatacgat gtctggtctg cgaaaggatt ctatgagcgc gacccgacga cagtttctct
     8161 cgacctccat gggcgcggcc gccgcagccg cccttcctcc cggcaaagac gatatctccc
     8221 tcgccaactg gtccatcgtg cagagtttcc gcgccggcaa gtggaaactc ctcgatatgc
     8281 cgcgcattct ccgcgacgtg ctgaagatca acgggcttga gtacgtcaac acattcttcg
     8341 agaatccgac aatagagtac ctgcggaaac tgaaacgcaa ctgcgaagac tacggaatga
     8401 cctcggtcct cgtgatggtg gacgacgagg gcaacaccgc ttccctcgac aaagccgagc
     8461 gcatgcaggc tgccgtcgcg caccgcaagt ggattgacgt cgcgcactac ctcggctgcc
     8521 atgctatccg ctgtaacctg cgcggcggcc caaaggaatg gcagcaggac aaggatgtcg
     8581 ccaagcgcgg agccgaggtc ttccgcgaca tcctcgagta cgcgaaagga tcgggtctca
     8641 acgtcctcat cgaaaaccac ggcggcgcct cctcagaccc agacgttctg ctcgaactga
     8701 tgcgtcttgt caatcacccg aaattcggca tgctggtcga taccggcaac tggaatcccg
     8761 gcgtcgaccg ctacgaggcc acgcgcaagc tcatccgcta cggcaagggc atgtcggtta
     8821 aaggcacgtg gggaccgaac aagcgggcgg acttcgacat ggagaagctc ctccaggtct
     8881 gcattgacgg cggctaccac ggctggtggg gcattgaagt cggatggcgc cgcccggagg
     8941 gcgttcaggc gactccggac gagatctggg agaacgagat caagtccgtg ctcgccgcga
     9001 agacggtcgt cgagcgcgtc gtattgaaga aggcgtaagc ggcctccccc cttacgccgt
     9061 tgagctgacc aggcgctgcg cgacagcgat gtcctctttc ttcggggtct cgccgtcgag
     9121 gaacttcggc agcgcgaacg tccgctcctc tttcagaacg gtgaggctca gcattgcgct
     9181 cggccgggtc gaggcaagtt ccggatcgag cgtccgcgct gcctcgaggt acagcatcga
     9241 tcccaggggg cgcagtgcct cggtgccgta gcggctggac agccgctcgt ggtacgtcct
     9301 tgtaatttcc gcgccgaatt cggccagtct ctcgatcgcc ttcgatggaa ccgccgcctg
     9361 ctgctcgcgg aagccctgga tctccttgag agaagccgcg gcgccgttcg cgatgatgtc
     9421 ttccagacgc agcagcgctc ccaggtgccc atcgagctcc tcccatgccg cagacgcgat
     9481 gaagtcgccg agctcatccg gcgcgaagaa tgccgcctcc gaagcctgtc ccgccgccag
     9541 aagccgctcc cgcgcgcccg cgatcaggac cgccagccgt tgcagcgtcc gctcatgaaa
     9601 caccatccgc cgccgcttgt ccgggtccct ccagtcccag tggactttct tctccttctt
     9661 gtcgatgaga tccgcgttta gccccgtcgc acggcccgaa acacgaatat tggtcgatgg
     9721 cgggatggcc gcgtagagca gcaacgccgc cgccggcatg ttttgccgta gccggttcac
     9781 atcctggaag tagtggaagg ggacgagttc ctttagcccg gcctgaagag cccgcgagac
     9841 cgccatcgat gcggcgcgca cgtcctccgc gtttctcggc gcgagccacg accggatcgc
     9901 tttcgccggt accgtgacct ccagcgcgag cagcacatct ccgaagtcgt ccgtcggccg
     9961 tccgagcacg tcctccacac agcgatcgag gtccgtcagc caggtcgaaa acgaggacgg
    10021 ccctccctgg gcaaattgct cggggaaata ccggtcgacg aacggcccca gttgccgctc
    10081 cagttcgacg cgccgcatgt tctcccgcgc catccggtat tggtacgccc attgcgccgt
    10141 cacctcgctg tagatccgca gcgcgccggc tgtctggacc ggaaccgccg cagccaaagc
    10201 gagttggctt ctcatccggt tccgaattga cacgtcatcc ttcgtgtcca attcgtagaa
    10261 cagaacccgc ccacgatctt ccaccgcccg cacgctcgcg aggctctggt tgagcttgtc
    10321 ggagcggaag ttgtagtagg gcatgttgac ctcgaccgag ccgcggcgcg tgatctcatg
    10381 cgtcagcacg ccctcctgga gcgtgacccc gtccacctga ttgaccagca ggacgtccag
    10441 gcggctctcc tgcaacaccg ccttcatcaa ctcagccgct ttcggcttct tcagatcgaa
    10501 cttggcatcc agcagcgcgg tacgctgggt ggctttctgg tacgcgtacg cgaacgaaaa
    10561 gtcgtagcgg cgcgccagtg cgttccgcgc ctgctcgtaa atctcctgcc gcttccggat
    10621 caccgcattg atcgcgttct tgatttcgtc gaggtcctcg aaccgaagct tccggtcgag
    10681 aaacgtggag agccggttca ccagccagcc ttcgatggcg ttaaagtcgg cccgcgtgat
    10741 cacgtttcgg acctggtcga tattcagctt ttcctcgatg aactgctgga gcttctcaat
    10801 cacgccgccg tcaaggacct ggagtatgtt gcttgcgacg ctccgtacct cctcactgcg
    10861 tccaaggaga tcaagcagac cgcggtcggc gacggctgca aggagacgtc cggaaggagt
    10921 gtcttcgatg cccaccgact ggagaaggcg ccgcagttcc ttctttcgcg cgtcctccct
    10981 cgttcccgcc agcaggctca gagaggcgac gagcgtcttt ctcgtctggc cctgcaggcc
    11041 gccgctctcg agcaacgccc agagtgttcc ggacacgcga gcgggcaacg agtcccactg
    11101 cttcagcgcc ttcaacaaga ctgtccgggc ctggttgaag ttttgctgtg gatcgacgcc
    11161 cgtcgttgtt ttgagcaggt cgagcccggt gtcccgggca aggcccgcga cggtgtcgga
    11221 caggttccgg ttggggtccg tccatttctc gatcagcaac agatccttca ccacctgctg
    11281 gccgtgaatg ccgaaaaccg cctttacgag accgtcgacg ttctcaggca gaccgtctgc
    11341 cactccttga acggacgccg acagattgag cccgaagttc caacctttct ttgagagctt
    11401 gaacagccgg aggcgcactt cggcggagtt tgcgtcgacc gactcgcggt cgagggtcac
    11461 cagatagcga ccgctcgcct caagcccgat tgtcgcttgg agtccggcgt cgagtttcaa
    11521 tccgatgtcc cccgtgaggc cgagtgccga agcctcgcga acgaaagtga aatcgtagcc
    11581 gagttttgcc gcaacgttca cggcgaccga cccctcaact tcggccacca gcatggttcc
    11641 gggctgaaga tcgctttccg agttgacgtg acggggcgga cgaaagctgg acaccatggc
    11701 tcgtatcgcc gtattcgccc cgagatcgcg tggaaaccgg tggatcacgg cgtacaactg
    11761 ctcgcgcctg gcctcaacgc cgaaagtgac cgaaccgagc atcccaatcg ggtgcgagcc
    11821 gtccacgttg ccttccacct cgtagccggc cacaaacagc aggtatcgca tgtcctgctg
    11881 gtcggtaaac gcgaggccga tctcgcccgc atccgcgagc gggagggcgc cgcggacctc
    11941 ctcgccggtg ttcaggaccg cgagccgagc gtgcgcgccg cccgaggccc gaaacgccac
    12001 ggcccctttc gcggcgttga accggatgtc tcttcctgcc tccgcctcga aggccacgcc
    12061 gccgagatcg atccggcggt ccggaaacgg cgagttggct aacactgcct gaaggacatc
    12121 ggcagcggtc gtgccgctga tctcgatgga tgcagcgcta cccggaacca gagtcgaggt
    12181 gtccaggttg aactttctcg tgagtggcgc tggcgtcaag agtttccctc ctcctatgct
    12241 ggaaccgcct ccctccggca gctctttcca gttgttgaaa tggtgtcacc aaccggcaga
    12301 acgtgtcaat gagggctggg ccaagcccga tcactgagcc gattttacac cgccaccagc
    12361 ttctcatagg ttacgctttg aggtaaactt ccgttagtcc ggagtttggg caacgggact
    12421 ctgactggtc tttctggggt tcaatctcac aattgaaaaa cacggcacaa gcgcgttgag
    12481 ttccgtccaa ccgggctcgg acactcgatg gggggaaaga caaatgcgat ctgtcgcgta
    12541 tggcgcgttg tttgccacga tctgcctttg cggatcggtt cccgggagtc gcccagaccg
    12601 taacaggcac ccttgacggc cggaccacgg atcaggctgg cgccgtcgta cccgacgtcc
    12661 gggtgtccgc cagagacctt cagaccggtc tcgaacgagc gcctgttcgg cgagcgccac
    12721 ttcgttcagt tccgggcaga aatgttcaac ttgacgaatc gggtccagtt tgagatgccg
    12781 gccttcgtgt ccgtggtcga cacgggcgtc gccggctcca tccacgattc ggcgagatca
    12841 ccggccagcg caacagtccc cgctcgatgc agatgtcgct ccgatacacg ttctgagtat
    12901 ttcgctcaca ccgattgcgt gccgcatcgt gattttcgtg gcacctgtct acctggggca
    12961 ccaggaggac aatgacgggc tgcgccgcgt ccgaagggcg cttcccacaa tttgtccttt
    13021 cttagaaagc ctataattcg ttcagcaatg gcaaccgacg ctgaaagcgc acgcacggct
    13081 ttcgcggaga gacttcgcca atcggcaggt ttgcggtccg gagcgctcgt gcgggccttc
    13141 gcaacggttt cccgagaaca cttcgtcggg ccaggtcctt ggaagattct tgtgccgccc
    13201 gatctgacgt accgtgatac gccggatggc agcccccacc atatatacga tgacgtgctt
    13261 gtggctatcg atggatcccg aggaatcaac aacggccagc cgagtgcgct cgcgcgatgg
    13321 ctcgatacgc tcgacctgac accgggtgaa cgttttctcc acatcggttg tggcgtcggt
    13381 tactacacag ctatcgccgc ggaggctctt tcccaaagcg gcaaggtgtt cggcatcgag
    13441 atcgacgctg aactcgcaga gcgtgccagc cggaacttga gcgggtacgc aaacataaca
    13501 gtcgtttgtg gcgatggcag ccgcttggct ggtgattcct acgatgccat tcttgtgaac
    13561 gccggggcca cggaagttca ggagggctgg attgaccagc ttcgccctgg cggacgcctg
    13621 ctcgtaccac tgactactcc gatacccggc atggatgttg gtgtggggca catgctcttg
    13681 ataacacggc atccgcgagg ttatgccgca gggtttgtgt cacccgtcgg catctttcac
    13741 tgtgcaggcg cgcggaccgg agagggtgaa gagttgctca agcagtccta cgctggagga
    13801 ggagaagata ccgtccgcag cttgctgagg gaagaacatc atccaagtgc gcagtgctgg
    13861 ctgcatcgcc ctggcttctg tctctcacgt ctgacaatcg aaggctgttt cagcacgtca
    13921 acccgaccgg cgtgatccgg ctatacaaag cgtgtccaga ctcgccagtc agaaggcaaa
    13981 accgaattgg cgtgaagccc cgtatcacac cgtgccgaag atccaactga gttgaccagg
    14041 agcgcggtca cccctcttcc gcagtgaaat atgcgcagtc ggggtggtgg aagaccaggg
    14101 cactgacact cgcctctgga tccatcatga atccgtccgt cagatgaacg aaaatctcgc
    14161 cgggattcaa caatttccag attccaagtt gatcttccag attaggacag gcgccgtatc
    14221 cgaagctgta ccgtttgcca cgataccgag acgtgaaccg accctgcatc gtcatggccg
    14281 gtgggtccgg gaatccccaa tcctcacgaa ttcgccggtg aagccattcg gcgcagccct
    14341 ccgccgtttc gagcgcgagc gcctgcaatg cgtgggcccg gaaatactcg cctgccgcct
    14401 tcgcctcttc ggctcgctca cggatcccgg ccccggccgt tgcgactaag atcgcgatgt
    14461 ggtctctccg tccctcctcc gggtcgagca catagtcgct caggcacagg ccgtccgccc
    14521 tcggctgccg tccgaatcgg aacgtgtgga gcgggcttcg agcgccaggg gcaaagagct
    14581 gaatggcgtt tccgtctctc tctgcctcga agaattgcca caccgcccgg accttcatga
    14641 agcccgccgc ccaacgcttg acctcctcca tctggtggta gagttccagc gccttctcgt
    14701 cgcgctcagc cagccgcttc tcgaagttgc ccttgaagcc gagatgcttc ccgaagagca
    14761 tatagggatt cacgtagctc cagacctcgg ccagttgcgg cacgtcgcgc actttccggt
    14821 cgagatacgg cgcgtcggga aggggcacgc cggtccggac cttgctgctg cgttgctccc
    14881 cgaccggagc ggccttgacg cctgcctgct cctcggccag cgcctcctga tgcgtgtggg
    14941 cgcgtaaaac ctcgtcccgc gccgccgggt ctacgatctg attcatcagc gccagaccgg
    15001 acatggcgtc cttcgcgtag cagactgctt cgccaaacgc cggagcgatc ttcagccggg
    15061 tgaacttctc cgagagcgcc gcgccgccca ccaacaccgg cacgcggatg ccggcatccc
    15121 ggaggtcctc cgccgtcacc accatctgct gcgcgctctt cacgagcagc ccggacaggc
    15181 cgatcgcgtc cgggtggtgc tcgtggtacg cccggatcag ctcttccggt ggcaccttga
    15241 ttccgaggtt gatcactttg tagccgttgt tgctcaggat gatgtccacg aggttcttgc
    15301 cgatgtcgtg aacgtcaccc ttcacggtcg ccaagagcac cttgccgcgg gtcgccgaat
    15361 cagcccgttc catgaactgc tccaggtggg tgaccgccgc cttcatggct tccgccgact
    15421 ggagcacttc ggccacgatc agctcattgt tgttgaacag ccggccgacc tcagtcatcc
    15481 ccgccatcag cggaccgttg atgatgtcga gcggcgccgc tcccaccgcg cgcttgagct
    15541 cgaggtctgc tatcagccca tccttcgagc cttcgatgat gtagttcgcc aggcgctgat
    15601 ccagcgggag aaggctcagc tcctttttct tgctcgccgc ccgcccccgg aagcgctcgg
    15661 aaatcgccgc gatgtggtgc tggttgatgg caatcttctg ttcgcgagtc tgctcccgcc
    15721 agtcctcgga cgccaatctg aggtcctcgg taggggcatc ggcaggaggc gtgttgaaca
    15781 gcaggtgctc ggccagcctg cgctcctgct ccggaatcga ggcaaagcgt tccagtttct
    15841 cggagttgac gatcgcaagg tcgagccccg ccttggtggt gtagtagaga aagacggagt
    15901 tgaccacttc tcgcgccgcg gcgggcaggc cgaatgagac gttggacacg ccgaggattg
    15961 tcttgacata cgggattttc tccttgatca gccgcaccgc ctcgatcgtc tccaccgcgc
    16021 cgccgacgta gttctcgtcg ccggtggcac acggaaagac gagaggatcg aggatcaggt
    16081 cctccggcgg gatgccatat ttctccgtca ggagccggac ggaacgctcg gccaccgcca
    16141 acttccgctc gcgggtgaat gcctgggcct gaaccgggtc ttcgtcaatc gtgccgacga
    16201 tcaacgccgc cccgtatgcg cgggcgatcg gacagacccg ctcgaacttt tcttcgccgt
    16261 cctcgagatt taccgagttg atgatgctct tgccttggca gtaggacagc gccagctcga
    16321 ccgccgcagg gtccgtggtg tcgatcatca ccggcacttt gatcttgcgg atgaggcgat
    16381 cgtaaaacgg cgggatatca cgaatttcct cgcgctcact agattgcagg cagacatcga
    16441 caatgtgcgc tccgttgcgc acctgccggc gcgcgatctc gctcgcctcc tcccaattct
    16501 ctccggcgat catctcttta aacgccctgg agccgatgac gttggtccgc tcgccgacga
    16561 tcagcggccg gttgctgtcg tcggcctcga cgagctcgat tcccgagtag aaggcgcgcc
    16621 caggccgacc gggcacggtg cgcggccgcc tgccttccac catctgcgcc agcatccgga
    16681 tgtgtgccgg cgtggttccg caacagcctc cgacgaggtt cagccagccg tgatcggcaa
    16741 accgctccag ttgcgcggcc agcgactcag gagtctcgcc gtacttcccc tcctcgttcg
    16801 gaagcccggc gttcgggtaa caggagatgc gcgtccatgc catctcgtgg agcgtacgga
    16861 tgtggtccgt catgaactcc gggccggtgg cgcaattcag gccgatcgac agcagatcca
    16921 tgtgggatac ggaggcccag aacgcatcgg ccgtctgacc ggcaagcatt gttcccgtcg
    16981 gttcgattgt tccggagacc atgaccggcg tcgagaatcc ggactcgcaa tcgagctgcc
    17041 ggaaggccag caacgccgcc ttcacgttcc gcgtgtcctg gcaggtctcg aggagcagaa
    17101 ggtcggcgcc gccgtcaatc agccctttcg cctgctcgta gtacacatcg cgaagctcgc
    17161 ggaaggtcac accgccggtc acggtgatcg ctttcgtcgt cggcccaagg gaccccgcta
    17221 cgaatcgagg cattccggag gtagagaact cagccgccgc ctggcgcgcg agcatggcgg
    17281 ccgtccggtt cagttcgtat gcccgatcgc ccaggccgta ctcggctagc acgagcttcg
    17341 ttccgccgaa cgtgtcggtt tcgacgatgt cggcgccggc ctcgaaatag gcgcggtgga
    17401 caccgagtac aacatccggc cgagtctgca cgagaatctc gttgcagccc tcaagcgacg
    17461 ctccgccaaa gtcggcggcg ctgaggtgcg cctgttgcag catcgtcccc atcgccccgt
    17521 cgaggacgag aatccgcctc tcgagggcgt ccagaagcgc cccccgcctg tcagcacgct
    17581 ctgtcatgaa gaatcggaag ttacttccga ttttatcagg cgagcacggt ctcagaacgt
    17641 gaacgccagt tgtccgaggt agcggaaggt cgaattcgcg ccgcgctgga gcggcacgaa
    17701 gaactgctcc gacgggttgc tggcgcgccg ctggttttgg atgaacagca ggttctgcca
    17761 ttgcaggaac cgcgtcagtc ccaggtcgaa tcgcagcgcg tgaactgcga tgttcacgcc
    17821 ggagccggtg ccgaggtcgt cgtcggtgaa ctgggagatc atcgagttgg cgtccttgat
    17881 agcgaactga tagagcgtcc gcaggtcccc cgctttgcgc accgcgccga ggttcaccga
    17941 tgccatcaag gcgtcgcgca agctggaagc cccggtattg cgcgcggcct gcaggtcgaa
    18001 ccaaaggggc atttcccgtc caccgatact gacacccttg cgctccagac ggtagcccag
    18061 gtgagcgatg tggaagtcag gcgcaaagta ggttgcgcca ccaggtagcg tagttccatt
    18121 tccagtgccg ccgatgggac cggacaggac gaaaccgagg tccttcgtca cgaccaccgg
    18181 gaagccggcc gctgtcgagg caagctgaat ctggttcggg ttgcggtaga tctggaaggt
    18241 cccgaggatc tggtggctcc atgcagggcc gaggtcgccg cgaaggatca cgccggggtg
    18301 gaacaaattt gcgtcacgga ccttctgtcc aacctgatac cccgcggcga caaacgggga
    18361 attggcgggg agtacgtaca ccgcgggatt ggacagaatg tactcgccga ccctgagctc
    18421 cacggtcttg aacccgagtg ggttcgatgc aaacgaaccc ttgaaagtct gatggaaccc
    18481 gttgaagcga acgtcgtcat cccagaggaa ccgcatgttg tcggcgaaaa cttcttccat
    18541 ccgcccgcca cgcagcgaga cgttggcgtt gggatggaag ttcacgtagg cttcggcgat
    18601 cgagatcggg tgcttggcca ctgtgccggc catgtcctgg tcgttcgtga tcgcgttgtt
    18661 gtacgggccg gtggacaact gaacgtggaa cctgaatcgt gggtccaaat ccttgtcgat
    18721 gttcaaacgc agccggtacc ggctccgcgc gttttgcaga ggagccgcaa cttcgttcga
    18781 tgaacgcgcc tggacatcga accggtatcg aaagtcgccg ctgaactgga acccaccaag
    18841 attcttcgac acggcctcgg tcttctttgc gagatcgtcg acgctggcgg acgacgcctt
    18901 tagagcggtc gccgcaggaa cggcggcggt cacgacgtca gaggatgact gctgagccag
    18961 ccggtcgagt gccctgccct gcgcctccaa cgtcttctgt aattccgcaa tttgcgcctg
    19021 ttgccgcgca agctgttcgc ggagggacat gatctcatcg gcatccgcac cctccgcccc
    19081 aaatgcggag aatgtgcata gagccgcggt caacatcaac caagtcagtg agattcgcat
    19141 gtgcagacta gccgggcgga agaagcattg tgaacttagc gcctttgcct aactggcttt
    19201 cgacactcac agttccgccc atcctttcga cgatgtgttt cacgatcgac aagccgaggc
    19261 cggttccgcc gacgtccctg gagcgggcct tgtccacgcg gtagaacctc tcgaaaatcc
    19321 ttggcaggtc atccgagggg atgccgatgc cattgtcggc gaccgtaagt cgcacgttcc
    19381 cctccgcggt gcatccggcc tcgacccaga cttcgccgtc cggacggtta aacttcaccg
    19441 cgttgtcgag caaattcacg agcgcctgtt ccagccggat cttgctgccc gtgacggcta
    19501 cgtcggccaa cccgccgcag tgaatctgaa cctgccgcaa ctttgcttcg gattccacgc
    19561 tgcgaagcgc gctctcgatt gcgccgcgaa cggagatgcg ctccggagcg ggcgcggctt
    19621 cgccgctttc cagctccgaa agcacgagaa ggtccgatgc gatggtgttc agccgcacgg
    19681 cgtgagactt gatgatttcg aggaacctgc ggttgtgttc tttgtcttcc agcgcacctt
    19741 ccaagagtgt ttcggcatat ccctgaatcg ccgtgagggg agtccgcaac tcgtgcgata
    19801 cgttggcgac aaaatctttt cgaacccgtt cgagcctctc caggtctgtg atgtcgtgca
    19861 gaatggcgat ggcgccacga cgcgttcgca cgtccagcgg ggctgcctgg atctcgaacg
    19921 accgaccctc ggcagcggca agttgcagcc gctccttctg gcttgcgcct gtcaccagca
    19981 cgcgcgtgag caggttgaga aacgccggat cccggatcag ttcgagaagc ggcagcctat
    20041 cgggaaccgg cacacgagcc ccgacagcac gggcgagaga ttcgttgcag aaggtgacac
    20101 acagctcgtg atcaacggcc aggactcctt ccaccatgct ggaaagcatc gcctcccgcc
    20161 tagctgattc gaggctcagc cgctcgatca ggtcctgcat ttgttccgcc atcccgttca
    20221 gcgagcgtgc gagagagccg agttcgtcgg ctggctccgg ttccattcgg tccgtaaacc
    20281 gcgcctgcac cagaccttcg gcaaactgct gaacacgacg aatgcggcca gtcacgtaac
    20341 gcgtgatgaa gtaagccact gcgagcgctg ccagggtcgc gatgagcgac acccggagaa
    20401 ttccccaccg cacgtccgca atcgccgcat tcaggttctt gagcggcacc gccacccgaa
    20461 acacgtggcc cggtttcgct ccgtacgtga gcggaatcgc cagatacacc aggtcgacgt
    20521 cgacagtgtc gctgtgccgg atcgagacgc cacgccgtcc ctccatcgcc tgtcgcacct
    20581 ccgggcggcc tgcgtggttt tccatcctcg ccggatctct ttccgagtcc gcgatcacca
    20641 cgccggccgg atcaattacc gtgatccgcg actgcgcccg ttgttgcgcc tgccgggacc
    20701 aggactggag ctcgggcgcc gggactacct gaacttcccc cgagagcagc cgcgcctgcg
    20761 aatccagttg ccgctcgacc gcgtcgactt cccgcgcaga aacaaatcgg gtcacgtaca
    20821 agtcgaggac gacgacggtc aagaggatca ggaggaatgc gccgagcagc agcttccgga
    20881 aaatagggct gctcagtgtc atcggatggg cccaccgatc ggggtctccc cgcgagtctc
    20941 cgcatgatgg cgcacgtcga tccccttgac gaagaacaca acgtcttccg caatgttcgt
    21001 cgcatgatcg gcgatccgct cgaggttgcg cgcgacgagg atcaagtcga gtgagcgggt
    21061 gatggtggac ggatcgcgtt gcatgaaacc gatgagttcc tggaagatgt tgtcccgcag
    21121 gtcgtccacg gcgtcgtcgg agagtaggac gccccgggcg aggtccgcat cgcgcttcac
    21181 gaacgcgtcc agactgcgcc gcatcatgga ttccaccagc cgcgccattt ggggaatgtc
    21241 gatcagtggc ttcacgggct cctgccggat gagcgacagc gagcgctcca cgatgttcac
    21301 cgcgaggtcg cccattcgtt ccagatcgtt gttgatcttg atgatcgagg tgagcaaacg
    21361 caggtccgta gccatcggct ggtagcgggc cagcaggccg gtcgcctgat catcgatctc
    21421 gatttccatc tggttgatgc gcgcttcgtc gaggagcacg cgctgggctc tcgtctcgct
    21481 acgttcggtc agcgacaaaa cactttcgtg aacggcggac tccaccaggc ccgccatctc
    21541 gagcaatcgt ttgatcagct cttccagttc ttcgagaaag tgactcaatg gcacctcgtt
    21601 acggccaagg atactttatc cgaaacggcc cgtaatgtag tcctctgttt ctttcctgga
    21661 cggattggtg aagatcgttt tcgtctggtc aaactcgatc agcctgccga gcagaaagaa
    21721 tcccgtatac tccgcgaccc tggcggcctg ctgcatgttg tgggtcacga tgacgatggt
    21781 gcagttttcc ttcagcctga ccaaaagctc ttcaattttt gccgtcgcta ttgggtcgag
    21841 ggcagagcat ggctcatcga gcagcagcac ctcggggtcg acggcaagtg cgcgtgcgat
    21901 gcacagccgc tgctgctggc cgccggagag ggccagcgcg gacttgtgaa gctggtcctt
    21961 aacctcgtcc cacagcgccg cccggcgaag actgtgctcg actgcatcct tgatcgcagc
    22021 ccggctgatg cctgtgttga tccgtaagcc gtaagccacg ttgtcgaaga tcgacttggg
    22081 aaacgggttc gacttctgga acagcatacc gacccgtcgc cggagttcga ccacgtccat
    22141 cgtcagcgtg ttctcgccgt cgagcagaac ctcaccttcc gcacgcgcgt gacggaccgt
    22201 ctcgtacatg cggttcaacg tgcggagaaa tgtcgacttt ccgcagccgg aggggccgat
    22261 cagtgcggtg accttctttt ccggaatctc caagtggata tcccagagcg ccctctgcgg
    22321 cccgtagtag aagctcagtc ccgatacgcg aagcttcggc acaaaatccg ctgtccgctg
    22381 cggggtggac tcgggctgag acttctggtc tcgggggccg atcatcatag tggagtcgtc
    22441 gtgggtaacc atcgtcaggc gctccggaac gacttgggcg aaagaatcaa ccgcgcggcg
    22501 acgttggcca caagcaccag ccccagcagg accagaccgg ccgcccatgc ctggcggtgc
    22561 cagtcttcat agggcgaaat cgcgtacgtg aagatcatca ccggcagcga ggcgaccggt
    22621 tgatcccacg atgtgctcca gtagcggttg ctgaaacttg tgaaaagcag cggagccgtc
    22681 tctccggcga ctcgcgcgac gccgagaatc gtcccggtca acaccccgcg aattcccgcc
    22741 ggaatgacga ccgttgcaat tgtccaccat ttggtcgcgc cgagagcgag cgcgccttct
    22801 ctcaggctgt gcgggaccgc tctaaggaac tgctcggtac tcctcacggt aatcggaatc
    22861 accatcaaac tcagtgccag cgctccagcg agcgtggaga accgcttcat cggcactacc
    22921 agcacggcgt acgcaaagat gccaatcacg atggatggca caccgttcag caggtccgtc
    22981 acgtaacgga tgacgaaagg gaat
//
LOCUS       DATIFQ010000527         4674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_950102, whole genome shotgun sequence.
ACCESSION   DATIFQ010000527 DATIFQ010000000
VERSION     DATIFQ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950102"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4674
                     /locus_tag="VLE22_26525"
     CDS             <1..>4674
                     /locus_tag="VLE22_26525"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HSB18035.1"
                     /translation="RLSESGLTIEQLVRSEGQRQAADQVSISNSIGSLRFLGAMDWRE
                     FVERMSVVERALREDPGGIYGKMDVATRDRYRHAVEKIAKRSSLSEGEVARKAIQLSG
                     EDASRRGRDERAAHVGFYLVDKGLPLLERTAQVRRTLFEELARRSRRVPLPLYLGTIS
                     LMTAVFTGFLLAKAYAGGTPDGLLVLMGCLSLVGGSQLAVALVNWLATLLVTPHPLPR
                     MDFSEGIPPESRTLVVIPTMLTSAPAVEDLVEALEVRFLANRDDNLHFGLLTDFRDAP
                     EETMPEDEPLVRLARKRIVDLNDKYRDAKSDAFFLFHRPRVWNPRERVWMGYERKRGK
                     LADLNSLLRGGSGDRFSLIVGRTAALSNVKYVITLDTDTQLPRDSARQFVGAMAHPLN
                     RAQVDDASLRVSEGYGILQPRVAVSLTGANRSRYARLFGSEPGIDPYTRAVSDAYQDV
                     FGEGSFIGKGIYDVDAFERALHGRFPENRILSHDLLEGCYARSGLLSDVQLYEEYPSR
                     YGADVSRRHRWIRGDWQIASWLVSRVPGPGGTRQRNPLSILSQWKIFDNLRRSLVPPA
                     LMLMLLLGWTVLSPLWLWTLAVIGILIMPPMAASVLELFRKPDDVLFRQHLAAAAPLA
                     GRHFAQAAFTLVCLPYEAFFSLDATMRTVWRVLVAHKRLLEWSPSGDPDRDRRANLVA
                     SCRSMWVAPAIAAAAGISLALSRPVALAAAGPLLALWFASPAIAWWISRPLAPRPPRL
                     MADQVLFLRKLARKTWAFFETFVGPEDHWLPPDNYQEYRVAVVAHRTSPTNMGLALLA
                     NLSANDFGYLSAGQLIERTENAFRTMESLERHQGHFYNWYDTQSLRPLPPLYVSTVDS
                     GNLAGHLLTLRAGLLALPDQKILGERWLEGLSDTLGVLVDAAGGAAKAQLVALHKDLA
                     AAFDSRPTTLSAAWQSLDRLAASAEDVIRVFEADSESPPAWWARAFARQCRGSLEELT
                     VLAPWILLPAAPEGLGGVPGIDGIPTLRELAGFEVRGEWPAELRRQIREASLRARERI
                     SAIDLLARQAGDLADMEVDFLFDKTSRLLAIGYNVGERRRDSSFYDLLASEARLCNFV
                     AIAQGQLPQESWFALGRLLTTAGGEPVLLSWSGSMFEYLMPLLVMPTYEDTLLDRTCK
                     AAVESQIQYGVRRGVPWGMSESCYNLVDVRFNYQYRAFGVPGLGIKRGLAEDLVVAPY
                     ASALALMVSAEEACLNLQRLAAEGAEGKFGLFEAIDYTPSRQRRGQSSAVVRAFLAHH
                     QGMSLLSLAFLLLDRPMQKRFESDPRFQATTLLLQERVPKATAFYPPAVELSEYRAAP
                     EGQMTPVRAFRSPDTPMPEVNLLSNGRYHVMVTNAGGGYSRWKDVAVTRWREDSTRDG
                     WGTFCYLRDVSSGEFWSTAHQPTLKRSEKYEAIFSEGRAEFRRRDHDFDTHTEIAVSP
                     EDDIELRRIRITNRSSRRRAIEATSYAEVVLASPAADALHPAFGNLFVQTEILPQRQA
                     ILCTRRPRSLEEPVPWMFHLMAVHGAETGEVSYETDRMRFIGRGNTVADPQAMRGPAA
                     LSGSQG"
BASE COUNT          747 a         1499 c         1580 g          848 t
ORIGIN      
        1 gcggctttcc gagtccggcc tgacgatcga gcagctggtg cggtcggagg gccagcgaca
       61 ggccgccgac caggtttcca tcagcaacag catcggcagt ctccggttcc tgggagcgat
      121 ggactggcgc gagttcgtcg agaggatgag cgtcgtcgag cgggctctgc gcgaggatcc
      181 cggagggatc tacggcaaga tggacgttgc cacccgggat cgttaccgtc acgccgtgga
      241 gaagatcgcg aagaggagct ctctatccga aggcgaggtg gcgcgcaaag ccatccagtt
      301 atccggcgag gacgcatccc ggagaggcag ggacgaacgg gcggcacacg tcgggttcta
      361 cctggtcgac aagggattgc cgctgctcga acgaacggcg caggtgcgcc ggaccctctt
      421 tgaagaactg gcgcgaagga gccgtcgggt tcccttgccc ctctatctcg gcacgatctc
      481 gctgatgacg gcggttttca cggggttcct gctggcgaag gcgtatgccg gcggaacccc
      541 ggatgggctg ctcgtgctga tggggtgcct ttcgcttgtg ggcggaagcc agctggcggt
      601 ggcgctggtg aactggctgg ccacgttgct ggtgacgccg catccgctgc cgcgaatgga
      661 cttctccgaa gggatcccac cggaatcgcg caccctggtg gtgatcccga cgatgctcac
      721 gagcgccccc gccgtcgagg atctggtcga ggcgctggag gtccggttcc tggccaaccg
      781 ggacgacaac ctgcacttcg gcctgctgac ggatttccgg gatgcgcccg aggaaacgat
      841 gccggaagac gaaccgttgg tgcggctggc ccggaagcgg atcgtagatc tgaatgataa
      901 gtacagggat gcgaagagcg acgcgttttt cctttttcat cgcccgcgcg tgtggaatcc
      961 ccgggagcgg gtctggatgg gctacgagcg caagcgaggg aagcttgcgg atttgaattc
     1021 gcttttgcgc ggcggctcgg gggatcgctt ttccctcatc gtcggcagga ctgccgctct
     1081 gtcgaacgtg aagtacgtga ttaccctgga tacggatacg cagcttccgc gcgactcggc
     1141 gcggcagttc gtgggagcca tggcgcaccc gctgaatcgc gcgcaggtcg acgacgcttc
     1201 gctgcgcgtc tccgaggggt atggaatcct tcagccccgc gtcgccgtga gcctgacggg
     1261 cgcaaaccgg tcgcggtacg cgcgtctgtt cgggagcgag ccgggcatcg atccgtacac
     1321 gcgcgccgtc tccgacgcct accaggacgt gttcggcgaa ggctccttca tcggcaaggg
     1381 gatctacgac gtggatgcct tcgagcgggc gctccatggg cggtttcccg agaaccggat
     1441 cttgagccac gaccttctgg aagggtgcta cgcccggtcg gggctattga gcgatgtgca
     1501 gctgtacgag gagtatccct cccgctacgg cgcggacgtg agccgccggc accgctggat
     1561 ccgcggggat tggcagatcg cgtcgtggct ggtgtcgcgc gtgcccggac ccggcgggac
     1621 ccgccagagg aatccgctct cgatcctgtc gcagtggaag atcttcgaca atcttcgtcg
     1681 cagcctcgtt cctccggcgc tgatgctgat gttgctgctg ggctggacgg tcttgtcgcc
     1741 gctttggtta tggaccttgg cggtgatcgg aatcctcatc atgccgccga tggccgcatc
     1801 ggtcctggag ctgttccgca agccggacga cgtgctgttc cggcagcacc tcgccgccgc
     1861 cgcacctctg gccggccggc acttcgccca agcggccttc acgctcgtat gcctccccta
     1921 cgaggctttt ttcagcctgg atgcgacgat gcgcacggtc tggcgggtcc tggtcgcgca
     1981 caaacgactt ctggaatgga gtccttcggg cgatccggac cgcgatcgcc gcgcgaacct
     2041 cgtcgcctcc tgccggtcca tgtgggtcgc tcccgcgatc gccgccgccg cggggatctc
     2101 cctggctctt tcgaggccgg ttgcgctggc cgcggccggg cccctgctgg ccctctggtt
     2161 tgcctccccc gcgatcgcct ggtggatcag ccggccgctc gccccccggc cgccccggtt
     2221 gatggccgac caggtcctct ttctccggaa gcttgcccga aagacctggg cgttcttcga
     2281 gaccttcgtc ggcccggaag accattggct gcctcccgac aactaccagg agtaccgcgt
     2341 cgccgtggtt gcgcatcgca cgtcgccgac caacatgggg ctcgcgctgc ttgcgaatct
     2401 atccgcgaac gacttcggct acctttcggc cggacaactc atcgagcgca cggaaaacgc
     2461 attccgcacg atggaatccc tggaacgtca ccagggccat ttctacaact ggtacgacac
     2521 gcaatccttg agaccgctgc ccccgctcta cgtctcgacg gtggacagcg ggaacctcgc
     2581 ggggcatctg ctgacattgc gggcgggcct gctcgcgctt cccgatcaaa agatcctggg
     2641 agagcgatgg ctggagggat taagcgacac gctcggtgtc cttgtggatg cagcgggagg
     2701 agcggcgaag gcgcagctcg tggcgcttca taaggacctt gccgccgcat tcgattcccg
     2761 gccgacgacg ctctcggcgg cgtggcaatc tctggaccgg ctggcggcgt ccgccgagga
     2821 cgtgatccgg gtatttgaag ccgactccga gagtccgccg gcgtggtggg cgcgtgcctt
     2881 tgcccggcaa tgccggggct ccctcgagga actgacggtc ctcgcgccgt ggatcctgct
     2941 gccggccgct ccggaggggc tcgggggcgt tcccggcatc gacgggatcc cgacgctgcg
     3001 cgagctggcg ggattcgagg tgcggggcga gtggccggcc gagcttcggc ggcagatccg
     3061 ggaagcaagc cttcgcgcca gggaacggat ttcggcgatc gacctccttg cacggcaagc
     3121 cggcgatctc gccgatatgg aggtcgactt ccttttcgac aagacgagtc gcctgctggc
     3181 catcggatac aacgtgggcg agcgcaggcg ggattcgagt ttctacgacc ttctggcgtc
     3241 ggaagcgaga ttgtgcaatt tcgtagcgat cgcccaggga cagctgccgc aggaaagctg
     3301 gtttgccctt ggacgcctcc tcaccaccgc cggtggagag ccggttctcc tttcgtggag
     3361 cggttcgatg ttcgagtacc tcatgccgct cctggtgatg ccgacctatg aagacacgct
     3421 gctcgaccgg acctgcaagg cggccgtgga aagccagatc cagtatgggg tgcgtcgcgg
     3481 cgttccgtgg ggcatgtcgg aatcctgcta caacctggtc gacgtccgtt tcaattacca
     3541 gtaccgcgcg ttcggcgtgc ccgggctggg gatcaaacgc ggactcgccg aggacctggt
     3601 cgttgcgccg tatgcgtccg ccctggcgct gatggtatcg gccgaagagg catgcctgaa
     3661 tctgcagcgg cttgccgcgg aaggtgcgga agggaaattc ggccttttcg aagccatcga
     3721 ctacaccccc tcccgtcaac gacgcgggca atcgagcgcc gtggtccggg ccttcctggc
     3781 gcatcaccag ggcatgagtc tcctgtccct ggcctttctg ctcctggacc gtccgatgca
     3841 gaagcggttc gagtcggatc cgcggttcca ggcgaccacg ctgctgctcc aggagcgggt
     3901 tccgaaggcc acggcgttct atccgcccgc cgtcgagctc tccgaatacc gcgcggcccc
     3961 cgaaggccag atgacgccgg tgcgcgcatt ccgcagcccg gacacgccga tgccggaagt
     4021 gaatctgctg tccaacggca gataccacgt gatggtcacg aacgcgggcg gcggctacag
     4081 ccgctggaag gacgtcgcgg tcacgcgctg gcgcgaagac agcacgcgcg acgggtgggg
     4141 cacgttctgt tacctgcgcg acgtgtccag cggggagttc tggtcgaccg cccatcagcc
     4201 gacgctcaaa cgatcggaga agtacgaagc gattttctcg gaagggcgag cggaattccg
     4261 ccgccgcgac cacgatttcg atacgcatac ggagatcgcc gtctccccgg aggatgacat
     4321 cgagctgcgc cggatccgca tcaccaaccg ttcctcgagg cgcagggcga tcgaggcgac
     4381 gagttacgcg gaagtcgttc tcgcatcgcc tgccgcggac gcattgcatc cggcgttcgg
     4441 caacctcttc gtccagacgg agatcctccc ccaacgccag gcgatcctct gcacccgccg
     4501 gccccgctcc ctcgaggagc cggtgccgtg gatgtttcac ctgatggcgg tgcacggggc
     4561 ggaaaccggg gaagtttcct acgagacgga ccgcatgcgg ttcatcggcc gcggaaacac
     4621 ggtcgccgac ccgcaggcga tgaggggacc cgcggcgctc tcgggcagcc aggg
//
LOCUS       DATIFQ010000528        12316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2481529, whole genome shotgun sequence.
ACCESSION   DATIFQ010000528 DATIFQ010000000
VERSION     DATIFQ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 12316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12316
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481529"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VLE22_26530"
     CDS             <1..246
                     /locus_tag="VLE22_26530"
                     /inference="COORDINATES: protein motif:HMM:NF036579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HSB18036.1"
                     /translation="RKNGARVEWDVTLPPNSTATIYVPARSGNDVTESGASAAEAQGL
                     RFSRMENGNAVFEAASGRYHFQSVTADSGGAAAVTGF"
     gene            complement(349..1188)
                     /locus_tag="VLE22_26535"
     CDS             complement(349..1188)
                     /locus_tag="VLE22_26535"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB18037.1"
                     /translation="MMLFRQQAKREFRELRAQPRLPAGIRVEVGWDERGKHRLEGGRC
                     IDISENGARVEIGEMVPVRTAIHLRFLDDVNFQADGIVRHSTGKGSIGVEFTQMMFCG
                     SLVPRKQPPLPIRLATPIVATSVFLIAVAWFSDILPKWRPSIDRSASSATFTPSPFFT
                     LGSTGADVQAVQGPPTASTSSTWSYGSSRVYFRRDRVIGWFSSRIAPLKVGADRVDPR
                     ETGAKAISRGSTAADVLALEGPPEQLAENVWRYGDSEIYFRDGRVVGWKSSLERPLRI
                     SAP"
     gene            complement(1402..3264)
                     /locus_tag="VLE22_26540"
     CDS             complement(1402..3264)
                     /locus_tag="VLE22_26540"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18038.1"
                     /translation="MFVFRTTILSLFAAWTLLFAEPPITLSVDLTDAPRRLTHASLSI
                     PAKPGPLTLLYPKWVPGEHGPTGPIADLVDLEIKAGGKPLAWRRDPVDMYAFHCEVPP
                     GAASVDVSLSFVSPPKTEGFSSGASATTELAVLSWNQVLLYPAGVAADAVIYKAKLQV
                     PKQWRYATALPVERESGDSVNFAPASLTTLVDSPLLAGRHFRTEDLNVGSAPRHYLHL
                     AADSREALELSQSVLSAYERLVRETGVLFGARHYGRYDFLVTLSDHVAHFGLEHHESS
                     DDRLPEMAMVDEPVRKLHAGLLPHEMAHSWNGKYRRPVGLATPDYQKPMLGELLWVYE
                     GLTTYLGDILTARSGLVTPDEFRQSLALDAAGMDLIAGRRWRPLADTAIGAQVLYGSR
                     DDWSNLRRGVDFYPEGALIWLDADVTIRTLSKGRKSLDDFCFRFFGGQSGAPAVKPYT
                     LEDVISELNAVQPHDWRAFFEERVYRPTPRAPLGGIEAGGWRLVYRESPSPYTNWRQD
                     ERREADLTWSIGLSLKDDGTVLDVVPGRAADKAGIAPATQMIAVNGRQFSTRVLRQAL
                     AAAKASSEPIELLMKNGEYYRTYRVDCHDGEKYPWLERDSSRPDLLTEIIRPRG"
     gene            3360..6386
                     /locus_tag="VLE22_26545"
     CDS             3360..6386
                     /locus_tag="VLE22_26545"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HSB18039.1"
                     /translation="MRILTIAAILILLVLTTLSGQQATIQEEKQVIKTYPFSGADPAP
                     IMTRSSMWGKGPRLYPYSFFDTLSYEGKDQAWKVIKLENPYIKVMVLPEEGGKVLGAI
                     EKSTGKEFIYYNKVRKFRHIALRGPWTSGGIELNFGIVGHSPATATPVDYVTRKNTDG
                     SVSVTVGAMDLPSRTHWRVTFTVPSDKAYFESKALWYNPQPLNGSYYVWMNAANRLGQ
                     DLEFIFPGDKHIGHNYRVPEKPWPVTADGRNLAFYKDHDDSDEGSFFIHGALQDFFGS
                     YWHNSKFGFGHWAYHEDTPGQKFFRWSLSGAGQIWEQLLTDSDGPYFEPQVGRLLDQN
                     DHEFFPPYTADHWREVWFPFKEIGPMVKATPYGALNARNTGDSIVLGFNALQNIDDDL
                     VVRAGGKEVYRERLKLEPMGVYQKKLALTAKPGELEVEIGNKLSYTDDPEAEKLKRPL
                     NFHEYDDNSLEALYQGGEREEKGRNYEVALDKYMAVLKQEPTHVRALTRVAELYTRRA
                     EYKKALEYATKALDYVMYDPDANYIYGVIARKMGNMVDAKETLGWAARSMKYRSGAYC
                     QLGEIYMMEGKWARAFDFLRRSLDYDAYNVKTYQVLSTAYRLHKEPAKARETLDKLLE
                     IDPLNHLARFEQYLLEPNGTSLENFRSMIRNELPHETYLETAMHYVNLGLPEDAQRVL
                     AAGPEQPQILYWRAYLSREKDPARSRELLQKASGSSPKLVFPFREESIPVFQWAEGMA
                     PNDWKAKYYLGLIYWGLRRHQDAIAALDECGNRPDFGTFYVCRAFLRKETDPEKALPD
                     YERAYETGKDDWRNWHHLAAFYNERGMNDKALQIAQAGAKQFPDADLMKIMLARTYLS
                     NGRSAECYSVLENATILPFEGQRDVHDLFVQCQTSMALELMKKGQYREAIQRLESAKE
                     YPVRLGTGKPHRADQRIQDFLMMLSYEKLGDQTNARQAMERVVAYMAGPSSDNADVLK
                     RRVDLWFKDSFPNQNGLAALQDLLKMVGSGRRRGGD"
     gene            6383..8203
                     /gene="ggt"
                     /locus_tag="VLE22_26550"
     CDS             6383..8203
                     /gene="ggt"
                     /locus_tag="VLE22_26550"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSB18040.1"
                     /translation="MTRLFVLALIAAFAVSAQRMVETVDVDPQPWKRTMRPVIRGTQY
                     AVSSMEPLATQAAERILLQGGNAFDAIVAGQAVLGLVSPASNGIGADATLLIFTGKEK
                     KVYSLNAEGTAPKLATIDWYKKNHEGKLPVDDTLLAATVPGAVDAWYIMLSRWGTKSF
                     GQVLAPAIELAERGFPVSEALAGGIAGSKKLSKYPSSVRVYFPNGVAPKAGEIWKNPD
                     LARTMKRLVEAERDSVSAGREAGLRAARDRFYKGDIAREMAKFSEENGGLFRYEDFAS
                     YTAKVEEPVSFNYRGYDVYKNPSATQGPAELFVLNILEGYDLKALGLNSAEYIHTSVE
                     AVKLAFADREKYLGDMDFIRIPWAGLLSKSYGEARRKLLDPEKASREFRPGVAEDFMQ
                     GFERLERPTDLTEAGKADHEGDTSYIVVVDKDGNGISFTPSLHSGFGSKVVLGELGFP
                     LNCRGDYFSLVPGHANALEPGKRPRSTLQSTLVTKNGKLYLLTGSPGGDDQCMRTMQT
                     FLNIVEFGMNVQQAIEAPRWSTRSFPASPFPHTMYPADMSVEARITLETRAELIRKGH
                     RMTVARPWSMNSSAAILVDPETGVLHAGADPRVNAYAVAW"
     gene            8214..8945
                     /locus_tag="VLE22_26555"
     CDS             8214..8945
                     /locus_tag="VLE22_26555"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSB18041.1"
                     /translation="MADGQTELKGKVVLVTGAAKRIGRGIALRLAREGARVAIHYGTS
                     EAEARRTAEECGGAPLFRANLESVAEIERMFSDVEQAMGGLYGLVNNAARFANIDPME
                     ITEADWDFIHDVNLKAVFFCCREAAKRMRNGGAGRIVNISSLGGIRPWAMHAHYCASK
                     AGVIMLTRALAKAFAPQITVNSVAPGVIPFEDIDEEGLRMIRRTPAARGGTPEEIAGA
                     VVYFLTASNFVTGQLLAVDGGLSQV"
     gene            8982..9887
                     /locus_tag="VLE22_26560"
     CDS             8982..9887
                     /locus_tag="VLE22_26560"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HSB18042.1"
                     /translation="MSRLNRRHFIQAAASAAAAVLPAAAQRPGGGKQMRLGLIVYAGN
                     NPESAIEKVHTLGFPTCQVGVGSFDPAIAKRLRAALDKYKVEATSLVSTGPPPEVYDF
                     YQGPATIGLVPRQHRQARIAQIKKTSDFGKLCGVNGIQTHCGFIPENPNDPLYKESVD
                     AIRTVAAYCKQNGQTFRCETGQETPITLLRAIKDVGLDNVGVNLDPANLILYGKANPV
                     EALDVLGPYVMGVHAKDGNYPTDPKQLGREVPIGQGKVNFPLFVKRLKEIGYRGPLTI
                     EREIEGPKQTEDVKAAKLYLEKLIG"
     gene            complement(9961..10965)
                     /gene="galT"
                     /locus_tag="VLE22_26565"
     CDS             complement(9961..10965)
                     /gene="galT"
                     /locus_tag="VLE22_26565"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HSB18043.1"
                     /translation="MPELRKDPVTGRWVIIASDRAMRPTDFVREQVIPKGGRFCPFCP
                     GNEQKTPPEVLAFRPHGGGRNEGGWTLRVVPNKFPALRVEGELNRQGEGIYDHMNGVG
                     AHEVIIETPDHLLTFGELSEKQVEDVFWAFRERVLDLKKDRRLIYILIFKNHGEAAGA
                     SLEHTHSQLIALPVVPKRVREELDGAKRYFDFKERCIFCDIVRQELDTGPRVVLETEH
                     YVVVSPYAPRFPFETWVVPKRHYSHFEDIETSSIQNLGWVMRATIRKIDKVLERPPYN
                     LMIHSAPIQEGAMTHYHWHIEIIPKLTKVAGFEWGTGFYINPTPPETAAKFLRDAGLV
                     "
     gene            complement(10988..11443)
                     /locus_tag="VLE22_26570"
     CDS             complement(10988..11443)
                     /locus_tag="VLE22_26570"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSB18044.1"
                     /translation="MIGLLHRICDRLRATARRRRWPHDQALGRRAEDMAHRLLRRSGF
                     TVVARNYRPRNSPGEIDLIAWHRDTLVFVEVKSRTTGEFGAPDRAIGREKRETLVRSA
                     LEYARRAGVPPDRIRFDVVNVVFSDPPAIDRIENAFRPAPPMYYNTFSP"
     gene            complement(11451..11975)
                     /locus_tag="VLE22_26575"
     CDS             complement(11451..11975)
                     /locus_tag="VLE22_26575"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB18045.1"
                     /translation="MRILDVGCGINKFPGAIGIDRNPRGRADVLCDLDRLPYPFADGS
                     FDELRAVHVIEHVSDVLRTLEEFHRLVRPGGRIFIVTPHYTDFSSFCDPTHRWHLNSF
                     SFRYFGENHGGFGYYSAFRLREISVRVKLLALWRCLGFEFLVNRFTRFRRFWEYYLCF
                     VVRGKVMEFEFEKL"
     gene            complement(12011..>12316)
                     /locus_tag="VLE22_26580"
     CDS             complement(12011..>12316)
                     /locus_tag="VLE22_26580"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSB18046.1"
                     /translation="RIGAFLMDRLHDWPRRFPYVGDVRGLGLMIGIEFVRDKATRERA
                     PKLRDRVVRMAFERGLLVLGAGQNTVRLSPPLTISRDQAAFAADTLEDCVRLAARGE"
BASE COUNT         2585 a         3567 c         3990 g         2174 t
ORIGIN      
        1 cggaagaacg gggcccgggt ggagtgggat gtaaccctac ctccgaactc gaccgcgacg
       61 atctacgtgc cggcacggag tgggaacgac gtgacggagt cgggcgcttc cgccgcagaa
      121 gcgcaaggtc tgaggttctc ccgcatggaa aacggcaacg cagttttcga agcagccagc
      181 ggacgctatc acttccaaag cgtgacggca gactcagggg gcgccgcagc cgttactgga
      241 ttttaagctc gacatagccg ccacagcggc gttggaacac ttcgaaatcc gattcctggc
      301 cggcgagcag gcacgattca gggctagtta caggcgccaa ctgacacatc aaggcgccga
      361 tatccttagg ggccgctcga gagaactctt ccagcccacc acacgtccgt cgcgaaaata
      421 gatttcggaa tctccgtacc gccacacatt ttcggccagt tgctcgggtg ggccttccag
      481 cgcgagaacg tccgccgcag tcgagcccct cgaaatcgct ttcgcgcccg tctcccgggg
      541 gtcgactctg tccgcgccga ccttaagcgg agctattcgc gaggagaacc agccgatgac
      601 gcgatcacgc cggaagtaga cacgggagga tccatagctc cacgtcgagc tcgtacttgc
      661 cgtcggaggg ccctgcaccg cctgaacatc ggctcctgtg gagcctagcg tgaaaaacgg
      721 acttggtgtg aacgtcgccg acgatgcact cctgtcgata gacggccgcc atttcggaag
      781 aatgtccgaa aaccatgcga cggctatcag gaacacggac gttgcgacga tcggggttgc
      841 gagcctgatc ggtaacgggg gctgcttgcg cggcacgaga gaaccgcaaa acatcatctg
      901 cgtgaactcc acgccgatgg agccttttcc cgtggaatga cgaacaatgc cgtctgcttg
      961 gaaattcaca tcgtctagaa aacgaagatg gattgcggtg cgaaccggaa ccatttcccc
     1021 aatctccact cgcgcgccat tctccgagat atcgatgcag cgcccccctt cgagtcgatg
     1081 tttgccgcgc tcatcccagc cgacctccac gcggatgccg gctggaagcc tgggctgagc
     1141 tctgagctcg cgaaactctc ttttcgcctg ttgtctgaag agcatcatgg ccggtgatcg
     1201 aattgcgcta gaagtcaccg gtgcttcgat tatcgggcgc cagatctgcg aatgaattgg
     1261 tacgaagatg ggtttaagta ccggcagggg actagccttt ctttcctagg tcatccgtac
     1321 cagaactcgc atcgagcaag gcgcagcggg gggtggcagg tggactcgaa ttagccgtta
     1381 cagacttcgc cgccttgcag gtcagccccg cggacgaatg atctcggtca ggagatcagg
     1441 gcgggaggag tcgcgttcga gccatgggta cttctcgcca tcgtggcaat ccacgcggta
     1501 tgtccggtag tactcgccgt tcttcatgag caactcgatt ggctcggacg aggctttggc
     1561 ggcggctaga gcctggcgaa gtacgcgtgt tgagaattgc cggccgttga cggcgatcat
     1621 ctgagttgcc ggtgcgattc ccgctttgtc ggcggcgcgt cccggcacga cgtcgagcac
     1681 tgtgccgtca tccttcaggc taaggccgat cgaccaagtc aggtcggcct cccgccgctc
     1741 atcctgccgc cagttcgtat agggagaagg cgactcccgg tatacgagtc gccacccgcc
     1801 cgcttcgata ccacctaggg gagcacgcgg cgttggcctg tagacgcgct cctcgaagaa
     1861 cgcgcgccag tcgtggggct ggaccgcgtt caattccgag atgacgtcct ccagcgtgta
     1921 gggcttcacc gcgggcgcgc cactctgacc gccgaagaag cggaagcaaa agtcgtcgag
     1981 agatttcctg cccttgctca gcgtgcggat ggtgacgtcg gcgtccagcc agataagggc
     2041 gccctcggga tagaagtcca cgccacggcg gaggttcgac caatcatcgc gtgacccgta
     2101 gagaacctga gcgccgatgg cggtgtcggc gaggggccgc cacctgcggc cggcgatcag
     2161 atccatgccg gctgcatcca gcgcgagcga ctgccggaac tcatccggcg taaccaggcc
     2221 gctgcgggcg gtgaggatgt cgcccagata agtggtgaga ccctcataga cccagagcag
     2281 ctcaccaagc atcggctttt ggtaatcggg tgttgccagg cccactggcc ggcgatactt
     2341 cccgttccag gagtgcgcca tctcgtgagg aaggagtccg gcgtgaagct tgcggacagg
     2401 ttcgtcgacc atcgccatct ccggcagccg gtcgtcgctg gactcatggt gttcaagccc
     2461 gaagtgcgcc acatggtcgc tcagggtgac cagaaaatcg tagcggccgt aatgccgcgc
     2521 gccaaacagt acgccggtct cccggacgag acgctcgtaa gcgctcaaga cgctctggct
     2581 gagttcgagc gcctcacggc tgtcggctgc gagatgcagg taatgccgcg gcgctgaacc
     2641 gacgttgaga tcttcagtgc gaaagtgccg gcccgcgagt agcggcgagt cgacgagcgt
     2701 agtgagcgac gccggtgcaa agttcacgga gtcacccgac tcgcgctcga ccggaagtgc
     2761 cgtcgcgtac cgccattgtt tcggtacttg cagcttggcc ttgtagatga cggcgtccgc
     2821 agcgacgccg gcagggtaga gaagcacctg gttccaactg agcaccgcaa gctccgtggt
     2881 ggctgaggcg ccactggaaa aaccctcggt cttaggaggg gagacgaagc tcagcgagac
     2941 atccacggac gcagcgccgg gcggcacctc gcagtggaac gcatacatgt ccaccggatc
     3001 gcgccgccac gcgaggggct ttccgccggc tttgatttcg aggtccacga ggtcggcgat
     3061 gggtccggtc gggccgtgct cgcccggtac ccactttggg tagagaagag tgagcgggcc
     3121 gggctttgcg ggtatggaga ggctggcatg agtgagccgc cgtggggcgt ccgtcaggtc
     3181 gacggacagc gttatgggag gctccgcgaa gagcagagtc caggcggcga agagcgacag
     3241 gatggtagtt cgaaagacaa acatgaggaa tctttatctt aaactcctca aggctggttg
     3301 cgttcaggga ggctggttca ttagactcgg tgagcgtagt ccacaggaga ccaccaggga
     3361 tgaggattct tactatagcg gccattctga tactgcttgt gctaacgaca ctgtcgggac
     3421 agcaggcgac gattcaggaa gaaaagcagg ttatcaagac atatcctttc tcgggcgccg
     3481 acccggctcc gatcatgacc cggtccagca tgtggggcaa agggccgagg ctttatccgt
     3541 acagcttctt cgacacgctc agctacgagg gcaaggacca ggcatggaag gtcatcaagc
     3601 tggagaatcc atacatcaag gtgatggtcc tgccggaaga aggcggcaag gtgctcgggg
     3661 cgatcgagaa gagcaccggc aaggagttca tctactacaa caaggtccgg aagttccgcc
     3721 acatcgcgct ccggggtcca tggacttcgg gaggcataga acttaacttc ggcatcgtcg
     3781 gccacagtcc agcgacggcc acgccggtgg actatgtgac gcgcaagaac accgacggca
     3841 gtgtaagcgt cacggtcggc gcgatggacc ttccgtcgcg cacgcactgg agagttacgt
     3901 tcacagttcc gtccgacaag gcgtacttcg agtcgaaggc cctctggtat aacccgcagc
     3961 cactgaacgg gtcctactac gtctggatga atgcggccaa ccgcctgggg caggacctgg
     4021 agtttatttt ccccggcgac aaacacatcg gccacaacta ccgggtaccc gagaaaccct
     4081 ggccggtcac tgcggatggg cgcaatctgg ctttctataa ggaccacgac gacagcgacg
     4141 agggcagctt cttcattcat ggcgctctcc aggatttctt cggaagctat tggcacaact
     4201 ccaagttcgg ttttggacac tgggcctacc acgaggacac tccgggacaa aagttcttcc
     4261 gctggtcgct ttccggcgcc ggccagatct gggagcaatt gctgaccgac agcgatgggc
     4321 cgtacttcga gccgcaggtc ggccgcctgc tcgaccagaa cgaccatgag tttttcccgc
     4381 cttacacggc cgatcactgg cgtgaagtgt ggttcccgtt caaggagatc ggcccgatgg
     4441 tgaaggccac gccgtacggc gccctaaacg cgcggaatac gggtgattcg atcgttctgg
     4501 gcttcaacgc gctccagaac atcgacgacg acctggtggt gcgcgctggc ggaaaagagg
     4561 tgtaccggga gcggctgaag ctcgagccga tgggagtcta ccagaagaag ctggcgctga
     4621 ctgcgaagcc gggcgaactg gaggtcgaga tcggaaacaa gctgtcgtac accgatgacc
     4681 cggaggctga gaagctaaag cggccgttga atttccatga gtacgacgac aacagtctcg
     4741 aggctctgta ccaagggggc gaacgcgagg agaagggtcg caactacgag gtcgcgctcg
     4801 acaaatacat ggcggttctg aagcaggagc cgacacacgt tcgagcactc acccgggtcg
     4861 ccgagcttta cacacggcgg gcggaataca agaaagcgct ggagtacgcc accaaggcgc
     4921 tcgattacgt aatgtacgat ccggacgcca actacatcta cggtgtgatc gcccggaaga
     4981 tggggaacat ggtggacgcc aaggagacgc tcggctgggc ggctcggtcg atgaagtatc
     5041 gctcgggggc gtactgccag cttggcgaga tctacatgat ggaaggcaag tgggcgcgcg
     5101 cgttcgattt cctgcgacgc tcgctcgact acgatgcgta taacgtcaag acgtaccagg
     5161 tgctcagcac cgcgtaccgc ctccataaag aacctgcgaa ggcccgcgag actctggaca
     5221 agctgctgga gatcgatccg ctaaaccact tggcgcggtt cgagcagtat ctgctggagc
     5281 cgaacggtac tagcctcgag aacttccggt cgatgatccg caatgaattg ccgcacgaaa
     5341 cgtacctcga aacggcgatg cattacgtga atctcggact tccagaagac gcgcagcgcg
     5401 ttctcgccgc gggaccggaa cagccgcaga ttctttactg gcgggcgtac ctctcccgag
     5461 aaaaagatcc ggcgcggagc agggagcttc tacagaaggc gtccggctct tcgccgaaac
     5521 tcgtttttcc gttccgcgag gagtcgattc ccgtgttcca gtgggcggaa ggtatggcgc
     5581 cgaatgactg gaaggcgaaa tactacctcg ggctgattta ctggggtctg agacgtcacc
     5641 aagacgcgat cgccgcgctc gacgagtgcg gcaaccgccc ggatttcggg acgttttatg
     5701 tttgccgcgc ctttctgaga aaggaaactg atcccgagaa ggcgcttccg gactatgaac
     5761 gcgcctatga gaccggcaag gacgactggc ggaactggca ccacctcgcg gcgttctaca
     5821 acgaacgcgg catgaacgat aaggcgttgc agattgcaca ggccggagcg aagcaatttc
     5881 cagacgccga cctgatgaag atcatgctgg ctcggaccta cttgagtaac gggcgttctg
     5941 cggaatgcta ctcggtgctc gagaatgcta cgattcttcc gtttgaaggg cagcgggacg
     6001 ttcacgacct gttcgttcag tgccagacgt cgatggcgct ggagctgatg aagaagggcc
     6061 agtaccgcga ggcaatccag cggctggaga gcgcgaagga gtacccggtg cggctcggca
     6121 ccggcaagcc tcacagggcc gaccagcgta tccaggattt cctcatgatg ctgagctatg
     6181 agaaactggg cgaccagaca aacgcccggc aggcgatgga aagggtcgtc gcttacatgg
     6241 ccggcccgtc aagtgacaac gcggacgttc tgaagcggcg ggtggatctc tggttcaagg
     6301 attcgttccc gaatcagaac gggctggcgg cgttgcagga ccttcttaag atggtaggca
     6361 gcggaaggag aagaggggga gattgaccag actctttgtt ctagctctaa tcgccgcatt
     6421 cgccgtctcg gcgcagcgga tggtcgagac ggtggatgtg gacccgcagc cgtggaagcg
     6481 gaccatgcgg cctgtcatcc ggggaacgca gtacgcggtc tcctcgatgg aaccgctggc
     6541 cacacaggcg gcggaacgga tactgctcca gggcgggaat gctttcgatg cgatcgtggc
     6601 agggcaggct gtgctcggcc tggtctctcc cgcatccaac gggatcgggg ccgatgccac
     6661 gctgctgatt ttcacaggta aagagaagaa ggtgtattct ttgaacgccg aggggacggc
     6721 gcccaagctc gcgacgatcg actggtacaa gaagaatcac gagggaaaac tgccggttga
     6781 cgacacgctt ctcgccgcga cggtaccggg cgcggtagac gcctggtaca tcatgctttc
     6841 acggtgggga acgaagtctt tcggacaggt gctcgccccg gcgatcgagc tggcggagcg
     6901 cgggttcccg gtatcggagg cactggcggg aggcatcgcc ggaagcaaga aactttccaa
     6961 gtatccgtcc agtgttcgtg tttactttcc gaacggtgtg gcaccgaagg cgggggagat
     7021 ctggaagaac cccgacctcg cccgtaccat gaagcgcctg gtggaggccg agagggacag
     7081 cgtatcggcc gggcgagaag ccggcttgcg tgcagcgcgc gaccggttct acaagggcga
     7141 catcgcgcgg gagatggcta agttctccga ggagaatggc ggactcttcc ggtacgagga
     7201 cttcgcgagc tacacggcga aggtcgaaga gccggtctcg ttcaactacc ggggatacga
     7261 cgtttacaaa aacccctccg cgacgcaagg gccggccgaa ctcttcgtcc ttaatatcct
     7321 tgaaggctac gacctgaagg cgctcgggct gaacagcgcg gagtacatcc acacttcggt
     7381 ggaggcggtg aagctggcgt tcgccgaccg ggagaaatac ctcggtgaca tggacttcat
     7441 ccggattcca tgggcggggc tgctctcgaa aagttacggc gaggcgcgcc ggaagctgct
     7501 cgatcccgaa aaggcgtcgc gggaattccg ccccggcgtg gccgaggact tcatgcaggg
     7561 tttcgagcgg ctggagcgcc cgactgacct caccgaggcc gggaaagcgg accacgaagg
     7621 cgacacgagc tacatcgtcg tggtggacaa ggacgggaac ggcatctcct tcacgccgag
     7681 cctgcacagc ggtttcggct ccaaagtggt cttaggcgag cttggcttcc cgctgaactg
     7741 ccggggcgac tatttctcgc tggtgccggg acacgcgaac gcactcgagc cgggaaagcg
     7801 cccgcggagc actttgcaga gcacactggt gacgaaaaac ggaaagctct acctcctgac
     7861 cggcagcccc gggggcgacg atcagtgcat gcggacgatg cagacgttcc tgaacatcgt
     7921 tgagttcggg atgaacgttc agcaggccat cgaagcgcca cgctggagca cgcgcagctt
     7981 cccggcgtcg ccgttcccgc acacgatgta tcccgccgac atgtcggtgg aagctcggat
     8041 tactctggag accagagccg agctgatccg caagggccat cggatgaccg tcgcccgccc
     8101 atggtcgatg aattcgagcg cggccatcct ggtcgatccc gaaacgggcg tgctgcacgc
     8161 cggcgcggat ccgcgtgtca acgcctacgc ggtagcatgg tgagcgcgaa cgcatggcgg
     8221 acggccaaac cgaactgaag ggcaaagttg tgctcgtgac cggcgcggcg aaacgaattg
     8281 gccgcggcat cgcgctgcgc ctcgcgcggg aaggggcacg ggtcgcgatt cactacggca
     8341 cttccgaggc tgaggcgcgc cgaaccgccg aggagtgtgg cggcgcaccg ctgttccgtg
     8401 caaatctcga gagcgttgct gaaatcgagc ggatgttcag cgatgtcgaa caggcgatgg
     8461 gcgggcttta cggattagtg aacaacgctg cgcggttcgc gaacatcgat ccgatggaga
     8521 tcaccgaggc cgactgggac ttcatccatg acgtcaacct aaaggcggtc ttcttttgct
     8581 gccgtgaggc ggcgaaacga atgcgcaacg gcggcgcggg acggatcgtg aacattagct
     8641 cgctcggcgg catccggccg tgggcgatgc acgcgcatta ctgtgcgtcg aaggccggcg
     8701 tcatcatgct gacacgggcg ctggccaaag ccttcgcgcc acagatcact gtgaactcgg
     8761 tggcgcccgg agtaatccca ttcgaggaca tcgacgagga aggactgcgg atgatccgcc
     8821 ggacgccggc agcgcggggt ggcacgccgg aggaaatagc gggcgcggta gtgtatttcc
     8881 tgaccgcctc caactttgtg accggccagt tactggctgt ggacggaggg ctgagccagg
     8941 tttgaatcga ctgatcagag atttcagagg cacccaaact tatgagcaga cttaacagaa
     9001 gacatttcat tcaagccgcg gcgagcgccg cggcggccgt actgccggca gcggcacagc
     9061 ggccgggcgg cgggaaacag atgcggctcg gactgatcgt gtacgcgggg aacaacccgg
     9121 agagtgcgat cgaaaaggtc cacactcttg gcttcccgac gtgccaggtc ggcgtgggct
     9181 cgttcgaccc ggcgatcgcg aaacggctgc gggcggcgct ggacaagtac aaagtcgagg
     9241 ccacatcact agtctcgact ggcccgcccc ccgaagttta cgacttctac caggggccgg
     9301 cgacgatcgg gcttgttccc aggcagcacc gccaggcaag aatcgcccag atcaagaaaa
     9361 cctccgactt cggcaagctt tgcggtgtca atggaatcca gacccactgt ggattcattc
     9421 ccgagaatcc aaacgacccc ctttacaagg agtccgtcga cgcgatccgg acggtcgcgg
     9481 cgtactgcaa acagaacgga cagacctttc gctgcgaaac ggggcaggag acgccgatca
     9541 cgcttctgcg agcgatcaag gacgtcggac tcgacaacgt cggcgtcaat ctcgacccag
     9601 ccaacctaat cctctatggc aaggcgaatc cggtggaagc tctggatgtg ctcgggcctt
     9661 acgtcatggg ggtgcacgcg aaggacggga actatccgac cgatccgaag cagttgggcc
     9721 gcgaagtgcc gatcgggcag ggcaaggtga acttcccgct gttcgtcaaa aggcttaagg
     9781 aaattggcta ccgcgggccg ctgacgatcg agcgggagat cgaaggcccg aaacagaccg
     9841 aggatgtgaa ggccgccaaa ctctacctcg agaaactgat cgggtaacgt ggtcaaggcc
     9901 accgctaaag gcaggccaca aacggcgatg gcctgcccca cgcgctcaag cagtgctgtg
     9961 ctagaccagg cccgcgtcgc ggaggaattt cgcggcggtc tcgggcggag tcggattgat
    10021 gtagaagccg gtgccccatt cgaagcccgc aactttggtg agcttcggga taatctcgat
    10081 gtgccagtgg tagtgcgtca tcgcgccttc ctggatcggc gcgctgtgga tcatcaggtt
    10141 gtagggaggg cgctccaata ccttgtcgat cttgcgaatc gtggcgcgca tcacccaccc
    10201 gaggttctgg atggatgacg tctcgatgtc ctcgaagtgc gagtagtgcc gcttagggac
    10261 gacccacgtc tcgaacggaa aacgcggggc atagggcgag acgacgacgt aatgctcggt
    10321 ttcgagaacg acccgcggcc cggtgtcgag ttcctgccgg acgatatcgc agaaaatgca
    10381 gcgctccttg aagtcgaagt agcgcttggc gccatcgagc tcctcacgga cccgcttggg
    10441 cacaaccgga agggcgatca actgtgagtg cgtatgctcg agcgaggcgc cagcggcctc
    10501 gccgtggttc ttgaagatca gaatatagat caggcgccgg tctttcttca ggtcgagaac
    10561 acgctctcga aatgcccaaa acacatcttc cacctgcttt tcggatagtt ctccgaaggt
    10621 cagcaagtgg tcgggagttt cgatgatgac ctcgtgggcg ccgacgccgt tcatgtggtc
    10681 gtaaatgcct tcgccctgac ggtttaactc gccttcgacg cgcagagcgg gaaacttgtt
    10741 cgggaccacg cgaagggtcc agccgccctc atttcggccg cctccgtgcg ggcggaaagc
    10801 cagcacctct ggcggcgttt tttgctcatt gccaggacag aacggacaga accggccacc
    10861 cttcgggatc acctgttcgc ggacaaaatc ggtcggccgc atggcccggt ccgaggcgat
    10921 aatcacccag cgtcccgtga ccggatcttt acgcagttcg ggcaaaaggc aagactcctt
    10981 ttctgaacta aggcgagaac gtattgtagt acatcggcgg agcgggacgg aaagcgttct
    11041 cgatccggtc gattgcgggg ggatcggaaa aaacaacgtt cacgacgtca aaacggattc
    11101 ggtcgggggg tacgccagcc cggcgtgcat actcgagtgc gctccgcacg agagtctcac
    11161 gcttctcgcg gccgatggcg cggtctggtg cgccgaattc gccggtcgtg cgggatttga
    11221 cctcgacgaa gaccagtgtg tcgcgatgcc aggcgatcag gtcgatttct cccggactgt
    11281 tgcggggacg ataatttctg gcgacaacag tgaacccgct ccggcggagt agtcgatgcg
    11341 ccatgtcttc ggcgcgccgg ccgagagcct ggtcgtgcgg ccatctccgg cggcgggcag
    11401 tggcacgcaa tcggtcacag atccggtgaa ggaggccgat cacggaatga ctaaagcttc
    11461 tcgaactcga attccatgac cttgccgcgg accacgaagc aaaggtagta ctcccaaaag
    11521 cggcggaagc gcgtgaagcg gttgaccaaa aactcgaatc cgaggcatct ccataaagca
    11581 agtagcttca cgcggacgga aatctcccgc agtctgaagg cggagtagta gccgaacccg
    11641 ccgtgatttt cgccgaaata ccggaacgag aagctgttga ggtgccatcg gtgcgtgggg
    11701 tcgcagaagg agctgaagtc ggtgtagtgc ggcgtcacga tgaaaatccg gccgcccggc
    11761 cgaaccagcc ggtggaactc ctcgagcgtg cggagcacat ccgagacatg ctcgatcacg
    11821 tggacagcgc gcaactcgtc gaaggagccg tcggcgaagg ggtagggaag gcggtcgagg
    11881 tcgcacagga cgtcggcgcg gccccgggga ttacgatcga tgccgatcgc tccaggaaac
    11941 ttgttgatgc cgcagccgac gtcgagaatg cgcatacaag ggccccggcc tggggctcgt
    12001 gggtgtcaga tcactcacca cgggccgcaa gccggacgca gtcctctagt gtatccgcag
    12061 cgaatgccgc ctggtcgcgg ctgatggtga gcggcgggct gagacgcacg gtgttctggc
    12121 cggctccgag cacgagcagc ccgcgctcga aggccatccg cacgacgcgg tcgcggagct
    12181 tgggagcacg ctcccgtgtg gctttgtcgc ggacgaactc gatgccgatc atgagtccga
    12241 ggccccggac gtcgccgacg taagggaagc gccgcggcca gtcgtggagg cggtccatga
    12301 gaaatgcacc tatccg
//
LOCUS       DATIFQ010000529         4702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_567805, whole genome shotgun sequence.
ACCESSION   DATIFQ010000529 DATIFQ010000000
VERSION     DATIFQ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567805"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..3443
                     /locus_tag="VLE22_26585"
     CDS             21..3443
                     /locus_tag="VLE22_26585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB18047.1"
                     /translation="MVGRRTVAVFALVVLLGVASSAFAQVGATVAQLNGTVRDETGAV
                     VVKATITLRDLGTNRTYTAASNEAGFYLVPNLAPGRYELKVAAAGFAGFTQTGIVLSV
                     GQTATVDVSLKAAAKGEEVVVTTEVPPIEPTRTEISQVIGTQQIQELPVSGRLFTDFA
                     LLTPGVATGRTSLGTTFTEFEITQISFGGQRSFSNIITVDGADFINANTGVQRATPPQ
                     ESVQEFRVVNNTFGTEYGRAIGGIVNVVTKSGTNELHGSIYEYFQNDAIASRSILQPE
                     GETPFVLRQNQFGATLGGPIVKDKTFFFVNYEGVRRGESPPLPPDFRNNFDAINAAKA
                     YLGLPPEPFDVLKTKDNDYGFVRLDHQLTTNHQLAIRYNVEDARDLNQLVGNTEDGGG
                     IGVPSGGRHLFIRDQSLVGTLISQLRPTLVNTFLAQYAKRTYDFPGATGEPDLSVPND
                     LEFGHNFGTLDAIYETRLQFSDSMAWVKGNHLIKFGYDSNYLYDSTIYPGFTPTRIIL
                     PDLNCLIEFANFVNIPGGPPLAPMPGPPCPLPNGQGPFPPFLNFGGVGATFYGVALPR
                     TGYVNGQFPLNNAVPLDTVNWSNAFDPSLRDSYRFKLDHGYYGFFIQDQWRVTPKFTF
                     NYGVRYDLETGLGQQIDSYHTAIQPRVGLAYSPDNKTVIRGGFGLFFDRNNMTFFFIT
                     GNQKTIPGFIPGITLPQVRTGAETGGWQLNLVVAPGFAPPPVACSTAPPPGLPCFSNA
                     ADVARSIITTGLYPQVFLAGDCPPTCTVGAGGMDRHHSQLPYAYQASLQIDREVGKGI
                     TLGAGYLFVGAHRLILGNGLNTACPVGTSKPNNPVIAQGWLNPDGTISDCEGTPELLV
                     GKPYFTGPPNFTGQEFTNGGFLDYNYAVVNANYHGLTLQASGKLSQYLRFNANYTWSH
                     TIDNGNFTTFINLPQNQFDNESERANSNQDVRHRFVANFTAEAPKDTFLRNFVYSSII
                     TIQSGRPFTLFTGGDSNGDTNPVTDRVGLIGRNSYIGDPLRTWDMRVSRYFKLSERVR
                     LDLGLDVFNLLNRANIDEVFSVYGSPVFCGGVPGHFGDATSLAIQQGASSVACPAFAD
                     LVASGDIPPAVAAGPPFAPPQFGIPPSPNPNFGTPRTVLNPRQLQFVLKISF"
     gene            3578..>4702
                     /locus_tag="VLE22_26590"
     CDS             3578..>4702
                     /locus_tag="VLE22_26590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18048.1"
                     /translation="MAPVRLRALQEGKLVTQLEYLFARSPFYQEKLGAAGIHREHFKS
                     LSDLSKFPFTTKEELRESQMACPPLGRHMAAKMEDVIRIHSSTGTTGQPSFVGVTRRD
                     AEMWTRVTARSFYTQGIRPSDIVIHAASLTMFVGGLPAKDAIEHIGATFVPVGAGASE
                     KLVLVARALHANALHCTPSYALYLADYVRREARMNPRDLGLEKLVCGAEPGAGIPSVR
                     AKMQEEWGARVTEGLGNADMAPIIFGECREQSGMHFCGQEYVLVEVIDPESGEVLPLE
                     PGVSGELVYTSLERECVPLLRFRTRDRVAVLGTSCACGRTSVRLRCVGRTDDMLIVLG
                     VNVFPSAVKDVVAGFYPRTTGEVQILLEQPGPAVPPPLKVV"
BASE COUNT          896 a         1467 c         1420 g          919 t
ORIGIN      
        1 ctttccccaa gaggtggctc atggtgggac gtaggactgt ggcggttttt gcacttgtcg
       61 tgctgttggg tgtggcaagc tcggcattcg ctcaagtagg cgcgacggtc gcccagttga
      121 acggcaccgt gcgggacgag accggggcag tggtggtcaa agcgaccatc actctgcggg
      181 acttgggcac gaaccggacc tacacagcag cctcgaatga ggccgggttc tacttagtgc
      241 cgaacctggc ccccgggcgc tacgagttga aggtggccgc cgccggcttt gcaggcttta
      301 cgcagaccgg gatcgtgctc agtgtgggcc agaccgccac cgttgacgtg agcctgaaag
      361 cagccgccaa gggcgaggaa gtggtggtga ctacagaagt gcctcccatc gagcccacac
      421 ggaccgaaat cagccaggtg atcggcacgc aacagatcca ggagttgcca gtgagtggcc
      481 ggctgttcac ggatttcgct ctcctcacgc ccggggtggc cacggggcgg accagcctgg
      541 gcaccacgtt caccgagttt gagatcacgc aaatctcgtt cggcggccag cgctccttca
      601 gcaacatcat tacggtggat ggcgccgact tcatcaatgc caacaccggc gtgcagcggg
      661 ccacgccgcc gcaggagtcg gtgcaggagt tccgggtggt gaacaacacc ttcggtaccg
      721 agtatgggcg ggctatcggc gggatcgtca acgtggtcac caagtcgggg accaacgagc
      781 ttcacgggtc aatctacgaa tactttcaga acgacgccat cgcctcgcgc tcgatcctgc
      841 aaccggaagg cgagactccc ttcgttctcc ggcagaatca gttcggggcc acactcggtg
      901 gtcccatcgt gaaggacaag acgttcttct tcgtgaacta cgagggcgtc cgacggggtg
      961 agtcgcctcc cctgccgccc gatttccgca ataattttga cgccatcaat gcggccaaag
     1021 cctacctggg acttccgccc gagccttttg atgtcctcaa gaccaaggat aacgactacg
     1081 gtttcgtacg cctggatcac cagctcacta ccaatcatca actggcaatc cgctacaacg
     1141 tggaggacgc acgcgacctg aatcagttgg tgggcaacac cgaagacggc ggcggcatcg
     1201 gggtgcccag tgggggacgg cacctgttca tccgcgatca atccctggtg ggaacgctga
     1261 tctcgcagct ccggccgacc ctggtgaaca cgttcctggc tcagtacgcc aagcggacct
     1321 acgattttcc aggggcgacg ggtgagcccg acctgagcgt tcccaacgac ctggagttcg
     1381 ggcacaactt cgggaccctg gacgcgatct acgagactcg cctccagttc tccgattcca
     1441 tggcctgggt caaaggcaac cacctgatca agttcggcta cgactccaat tacctgtatg
     1501 actccaccat ctatcccggg ttcacgccaa cccgcatcat cctgccggat ttgaactgtc
     1561 tgatcgagtt cgccaatttt gtgaacatcc ccggtgggcc gccgctcgcg ccgatgcctg
     1621 gtccaccgtg ccctctcccc aatggccagg gtccctttcc tccgttcctt aattttggcg
     1681 gcgtgggggc cactttctat ggcgtcgcac tgcccaggac cggctacgtc aacggtcagt
     1741 tcccgctcaa caacgccgtc ccactggata cagtgaattg gtcgaatgcg tttgatccct
     1801 cgctaagaga cagctatcga ttcaagctcg accacggcta ctacgggttc ttcatccagg
     1861 accagtggcg cgttactccc aagttcacct tcaactacgg cgtgcgctat gacctggaaa
     1921 ccggcctcgg ccagcaaatc gattcttacc acacggccat tcagccgagg gttgggttgg
     1981 cgtattcccc ggacaacaag acggtgattc gcggtggctt cgggctattc ttcgatcgca
     2041 acaacatgac cttcttcttc attaccggca accagaagac gattcccggg ttcatccccg
     2101 gcatcacgct gcctcaagtt cgaaccggag cggagacagg cggatggcaa ctgaatctgg
     2161 tggtggcgcc agggtttgca ccgccacccg tggcgtgttc gacggctccg ccgccaggct
     2221 tgccatgctt ctcgaacgcg gctgacgtgg cccgcagcat catcacaacc gggctctatc
     2281 cccaggtgtt tcttgccgga gattgcccgc ccacgtgcac ggtcggcgcc ggcgggatgg
     2341 atcgccacca cagccagctc ccctatgcct atcaggccag cctgcagatc gaccgcgagg
     2401 tgggaaaggg gatcaccctc ggtgccggat atctgttcgt cggggcacat cgcctgatcc
     2461 tggggaatgg tttgaacact gcctgtccgg tgggcaccag caagccgaac aaccccgtga
     2521 tcgctcaggg gtggctcaat ccggacggca ccatcagcga ttgtgaaggg actccggagt
     2581 tgttggttgg caagccctac tttaccgggc cgcccaactt caccgggcag gaattcacca
     2641 acggcggttt cctcgactac aactatgccg tggtcaacgc caattaccac ggcctgacat
     2701 tgcaggccag cgggaagctc agccagtact tgcgctttaa tgccaactac acctggtcgc
     2761 ataccatcga taacggtaac ttcaccacgt ttatcaacct cccccagaac cagttcgaca
     2821 acgaatcgga acgggccaac tcgaatcagg acgttcgcca ccggtttgtg gccaacttca
     2881 cggcggaggc tcccaaggac accttcctgc gcaactttgt gtacagcagc atcattacca
     2941 ttcaatccgg gcgccccttt acgctcttca ccggcggtga ttcgaacggc gacaccaatc
     3001 cggtgacgga ccgtgttggc ctcataggga ggaactcgta catcggcgat cccctgcgga
     3061 cctgggatat gcgcgtctcc cggtacttca agctgagcga gcgagtgaga ctggatctgg
     3121 gtctggatgt gttcaacctg ctcaaccggg ccaacatcga cgaggtgttc agtgtctacg
     3181 ggtcgccggt attttgcggg ggcgttccgg ggcacttcgg ggatgccacg tccctcgcga
     3241 tccagcaggg agcgtcatcc gtagcctgtc cggcattcgc cgatctggta gcatccggag
     3301 acattccgcc ggcggtggcg gccgggccgc ccttcgcccc gccgcaattc ggcattccac
     3361 cgtccccaaa tcccaatttc gggacgccca gaactgtgct caacccacgg cagttgcagt
     3421 tcgtgctcaa gatcagtttc tagttgtcgg gcgggcgaga cggtcggaag cgtggcggtt
     3481 ccggggcgac gtgttaacct accgctcgcc cgctggggca cggctctcct cttttccgat
     3541 gcaggctgac gaataccttg acccagaaat cgagaccttg gccccggtac gactgcgggc
     3601 gttgcaggaa gggaagctag tcacgcaact ggagtatcta ttcgcacgat cgccgttcta
     3661 tcaggaaaag ttgggggcgg ccggcatcca ccgcgaacac ttcaagtcgc tcagcgacct
     3721 gagcaagttt ccgttcacca ctaaggagga gttgcgggaa agccagatgg cctgccctcc
     3781 gctgggacgg cacatggccg caaagatgga ggacgtcatc cgcattcact cgtccaccgg
     3841 taccaccggc cagcccagct ttgtgggcgt gacgcggcgg gacgccgaga tgtggacgcg
     3901 ggtgaccgct cgctccttct atacgcaggg aattcggccc tcggatatcg ttattcatgc
     3961 ggccagcttg accatgttcg tcggcgggtt gccggccaag gatgccatcg agcacattgg
     4021 cgccaccttc gttcccgtag gagccggcgc atccgagaaa ttggtgctgg tggcccgggc
     4081 gctgcacgcc aatgcgttgc attgcacgcc ttcctacgcc ctgtacctgg cggactacgt
     4141 gcgccgggaa gcccggatga atccccgaga cttgggcctg gagaagctgg tctgcggagc
     4201 cgagcccggc gctggcatcc cctcggtccg cgcgaagatg caggaagagt ggggagcgcg
     4261 ggtcaccgaa ggcctgggga acgcggacat ggcgccgatc attttcggag aatgccgtga
     4321 gcaatccggg atgcatttct gcggtcagga gtatgttttg gtcgaggtca tcgacccgga
     4381 gagcggggag gtgctgccgc tcgagcccgg ggtctcgggc gaactggtgt acaccagcct
     4441 ggagcgagag tgcgttccct tgcttcgctt tcgcacccgg gatcgcgtcg ccgtcctggg
     4501 aacgtcgtgc gcatgcggac gcaccagcgt taggctaaga tgcgtcgggc gaaccgacga
     4561 catgttaatc gtcctggggg tgaacgtgtt cccctcggcg gtgaaggatg tggtggcagg
     4621 gttctacccg aggaccacag gagaagtcca gattctgctc gagcagcccg ggcccgcggt
     4681 gccacctccc ttgaaggtgg tg
//
LOCUS       DATIFQ010000530         5090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1525363, whole genome shotgun sequence.
ACCESSION   DATIFQ010000530 DATIFQ010000000
VERSION     DATIFQ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525363"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VLE22_26595"
     CDS             <1..966
                     /locus_tag="VLE22_26595"
                     /inference="COORDINATES: protein motif:HMM:NF045042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18049.1"
                     /translation="KTPWTAMVSDFAKSSTRIHMSVAYLDGKHPSVLTQTGLYENEII
                     EAYDARLKKLWEFKSFAETNGSGSHHIDIADVDGDGIDEVFNGTTLLNADGTMRWSLY
                     REHPDIVAIKHVLPGSKDRQVYYVVESSVHAGVYVVDAKTGKIHWKVNREDDPRWTHG
                     HVGWASDIWEGSPGMELYANRDGHLSKDTVLFSAAGKLLLSPFPAGWRPVNWTGGAVR
                     ELMSGDGRLLARFNGQSVETLAIPGPNEMKNGSCGMTADLAGDYRDEVVCFGTTAEGA
                     QAVFVYANTEPAAKREVTRTADREYALWLARNMGGGYASYFEWQP"
     gene            977..2932
                     /locus_tag="VLE22_26600"
     CDS             977..2932
                     /locus_tag="VLE22_26600"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18050.1"
                     /translation="MRLAIALIVALAPAVGRAQQGWWMEEPIRLIQTNLRETDSTLEA
                     GRLAQQVAEFPANVLLFNMGGIAAHYHPRVEFHYASPHLPPGRDMFGDTLREAHKRSI
                     RVIGRFDLSKTPKHVFDAHPEWFFKRVNGEPAIYNGLYSTCINGGYYREHAKKILGEA
                     LERYEVDGLFFNMFGNPSSDYSGNPMGPCQCDNCKTRFQARYGRPLPVSPDDDYRRFL
                     TDSAHEVAAMFAELIHSKRPKAAFLTYVQQHTDGIMSESNTAVGRPLPLWPYSASDNV
                     NRARNSEPTKMAFNLCMSFVDYMWRFVTVPPAEIRLRLYQSMAHGSGVLLNMHGTMDQ
                     EDRSALEAARPVFQWHARHEDLYTGQESAARVLLLGGRQSSYRGLFRILSEQHIPFVV
                     SDNLKWLEDGRTFDMVISPDGAPAALEHYVRDGGRLLVCGPKPPAWKVAGVGSLRPHT
                     QGYWRIRDHTLFPSLKRTNLLFLDGEYTELSPVAKPLLTLIPPAMFGPPEKVWVDKLE
                     TDSPGLLLTDYGKGRLAYVPWDVGGLYYRHSSEGHSGVVADLINYLFPAGRQLITNAH
                     PLVEITVMRQPRKNRTLVHFVNVSGHSGTAYFAPLPMRDIRVELAAEFTKARSVTLGR
                     ELPVMRKDRYGVFSLPEFAEYDVVLLE"
     gene            2988..3227
                     /locus_tag="VLE22_26605"
     CDS             2988..3227
                     /locus_tag="VLE22_26605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18051.1"
                     /translation="MSTWVLVCYGGAALVALLLLLVFRAKSWYWHVLSILAALVVGLV
                     PIPPQLNTPQGSLIIGSVFTFLLLWGVAAPFFRRT"
     gene            3790..>5090
                     /locus_tag="VLE22_26610"
     CDS             3790..>5090
                     /locus_tag="VLE22_26610"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HSB18052.1"
                     /translation="MVTKFLLSEAQLPSAWINVLPSLPVPMDPPLHPGTLKPIGPEDL
                     APIFPMALIEQEFASKPEIDIPGEVLDIYRLWRPTPLMRARRLEKALDTPAHIYYKYE
                     GTSPAGSHKPNTSIPQAFYNKQAGIKRIATETGAGQWGSALALACRLFGMECTVYMVK
                     VSYQQKPYRRMMMNTWGAEVYASPSEHTNAGRQVLAAYPDSPGSLGIAISEAVEDAAT
                     HPDTNYSLGSVLNHVNLHQTVIGQEAKLQMAMAGEDPDVIIGCFGGGSNFGGIALPYV
                     LDKFNGSAVRLMAVEPAACPTLTKGAFTYDFGDIAKLTPLLRMYTLGHDFVPPGIHAG
                     GLRYHGASPLLSHLINQGYIEARSVLQIPTFEAAVLFSQTEGLLPAPESAHAIRAAID
                     EAIAAREEGRPRVILFNLSGHGHFDLASYEAYLSGKLQDYE"
BASE COUNT          965 a         1655 c         1545 g          925 t
ORIGIN      
        1 aagacgccat ggactgccat ggtctccgac ttcgccaagt cgtctacccg gatccacatg
       61 tcggtggcct acctcgacgg aaaacacccg tccgtcctga cccagaccgg cctttatgag
      121 aacgagatca tcgaggccta cgacgcccgc ctcaagaagc tctgggagtt caagagcttc
      181 gccgagacga atggcagcgg ctcccatcac atcgacatcg ccgacgtcga cggagacggc
      241 atcgacgagg tcttcaacgg caccacgctg ctgaatgcgg acgggacgat gcgctggtcc
      301 ctctaccgcg agcaccctga tattgtggcc atcaagcacg tgctccccgg ctcaaaggac
      361 cgccaggttt actacgtcgt cgaaagcagc gtccacgcgg gcgtttacgt cgtggatgca
      421 aaaaccggca agattcactg gaaagtgaac cgcgaggacg accctcgttg gacacacggg
      481 cacgtcggct gggcctcgga tatctgggag gggtctccgg gcatggaact gtatgccaac
      541 cgcgacggcc atctctcgaa agacaccgtc ttgttctccg ccgcaggcaa actcctcctg
      601 agcccattcc cggcgggctg gcgcccggtt aactggaccg gtggcgcggt ccgagagctg
      661 atgtccggag acggccggct cctcgcacgg ttcaacgggc agtcggtcga gactctcgcc
      721 attccagggc cgaacgaaat gaaaaacgga agttgtggaa tgacagccga cctggccggc
      781 gattaccgcg acgaggtggt ctgcttcggc acgacggccg aaggcgcgca ggccgtgttc
      841 gtctatgcaa acaccgaacc cgcggcgaaa cgcgaggtca cccggacagc ggaccgcgag
      901 tatgcactgt ggcttgcccg caacatgggc ggagggtatg cctcgtactt cgaatggcaa
      961 ccataaggag ggaccgatgc ggctagcaat cgcactcatc gtcgcgctcg cgccggccgt
     1021 tggacgcgcc cagcagggct ggtggatgga ggagcccatc aggctgattc agaccaacct
     1081 ccgcgaaacc gactccacgc tcgaggccgg ccgtctcgcg caacaagtcg cggaattccc
     1141 cgcgaacgtc ttgctgttca acatgggtgg catcgcggcc cactaccatc cccgcgtcga
     1201 gttccactac gccagcccac atctgccgcc cgggcgcgac atgttcggcg atacgctgcg
     1261 ggaagcccat aaacggagca tccgcgtgat cgggcgtttc gacctgagca aaacgcccaa
     1321 gcacgtcttc gacgcccacc cggaatggtt cttcaagcgc gtcaacggcg agccggccat
     1381 ctacaacggg ctctactcga catgtatcaa cggcggctac tatcgtgagc acgcgaaaaa
     1441 aatcctcggc gaggctctcg agcgctacga agtagacggc ctcttcttca acatgttcgg
     1501 caatccatct tccgactaca gcggcaaccc gatgggaccg tgtcagtgcg acaattgcaa
     1561 aacacgcttc caggcgcgat acgggaggcc gctcccggtg tcgccggacg acgactaccg
     1621 ccggtttctg accgactccg cgcacgaagt ggcggcgatg tttgccgagc tgatccattc
     1681 caaacggccg aaagccgcgt tcctcaccta cgttcagcag cacaccgacg ggatcatgtc
     1741 cgagtccaac accgccgtgg ggcgtccgct gccgctctgg ccgtactccg ccagtgacaa
     1801 cgtgaaccgg gcgcggaact ccgagccaac gaagatggcg ttcaatctct gcatgagctt
     1861 cgtggactac atgtggcgct tcgtcaccgt gccgccggct gagattcgtc tccgccttta
     1921 ccaaagcatg gcgcacggct cgggcgtgct gctgaatatg cacgggacga tggaccagga
     1981 ggaccggagc gcgctggagg ccgcccgtcc ggtcttccag tggcacgcgc ggcacgaaga
     2041 cctgtacacg ggccaggaga gcgcggcgag agtgctgctg ctcggcgggc gacagtcgag
     2101 ttatcgcggc ctgttccgaa tcctgagcga gcagcacatc ccgttcgtcg tctcggacaa
     2161 cctcaaatgg ctggaagacg gacgcacgtt cgacatggtc atctcgccgg acggcgcgcc
     2221 cgccgcgctc gagcactacg tccgtgacgg cggacgtctg ctcgtctgtg ggccaaagcc
     2281 gccagcgtgg aaagtggccg gcgtcggctc gctgcgcccc cacacacagg ggtactggcg
     2341 aatccgcgac cacactctgt tcccctccct caaacgcacg aacctgctgt ttctggacgg
     2401 agagtacacg gagctgtcgc cggtggcgaa gccgttactg acgctgattc cgccggcgat
     2461 gttcggtccg cccgaaaagg tctgggtgga caagctggag acggattcgc ctggactgct
     2521 ccttacggat tatggcaagg gccgcctggc ctatgtgcct tgggatgtgg gcggacttta
     2581 ttaccgccac agttctgagg gccactcggg tgtggtggcg gacctcatca actatctgtt
     2641 tcccgccggt aggcagttaa ttacgaatgc gcacccactt gtggagatca cggtcatgcg
     2701 ccagccgaga aagaaccgga cgctcgtcca cttcgtgaat gtttcggggc actccggcac
     2761 tgcctacttc gcgcctctcc ccatgcgcga cattcgggta gaactcgccg cggagttcac
     2821 caaagcccgc tccgtcacgc tcggccggga gcttcccgtc atgcgtaagg atcggtacgg
     2881 tgtgttctcg ttgcccgagt tcgcggagta tgacgtggtg ctgctggagt aagccggtcc
     2941 ggtatcgcgc cctcggatat agaatggagg gcggagaaaa tctgtccatg tccacttggg
     3001 tccttgtctg ctacggaggg gccgcgctcg tggccctgtt actgctccta gtcttccgag
     3061 cgaaatcgtg gtactggcac gtgctgagca ttctggcggc actggtggtt ggccttgtgc
     3121 ccatcccccc gcagttgaac accccacagg gcagcctgat catcggatct gtgttcacct
     3181 tcctgctcct gtggggagtg gcggccccgt tcttccgccg cacgtaatcg aatcgccgtg
     3241 aggcgaaaga tcagaggagg aagaccatgg cgcgcgagcg tttgcttctg attctcccct
     3301 tttctggcgg ccgtctgggt aactgctgcc tgggcaacca cctatgaagt cggctctgga
     3361 aagccttaca cgtccatcgg cgccgtgccg tgggctgcgc tccagccggg cgacacggac
     3421 caccaactcg cggcaggctc ggccgccatc gacgccggcg ggctgttgaa cgtcctcgtt
     3481 ctgcctgcga atgacgttct ccgccagtac gtgaaacatc agtccagcga ggcccgtccg
     3541 caagaccccc gctcgacatc ggcgccttcg aattcgcccc gtaggccggt ggtggaggcc
     3601 atcgtcttgc gtggcctgag atccccgtct tggtggagcc agtcgccaga tcggccatgc
     3661 cggcagtctt gctggcagcc tccaggttcg ttgtggaaag ctcttcttgt gccccgcccg
     3721 cgagcccgtt ctaagaactg cgttagaatg agcgtttact tttcacccga aggagtctga
     3781 ttcaccccta tggttacgaa attcctgctg tccgaggcgc aactgccctc cgcctggatc
     3841 aacgtgctgc cgtcgctgcc ggtgccgatg gacccgccgc tgcatcccgg gacgttgaaa
     3901 ccgatcgggc ctgaggatct cgcgccgatc ttccccatgg cgctcatcga gcaggagttc
     3961 gcgtccaaac ccgagatcga catcccgggc gaggtgctcg acatttaccg cctctggcgg
     4021 ccaacgcccc tcatgcgggc aaggcggctg gaaaaggccc tcgatacacc ggcgcacatc
     4081 tattacaagt acgaggggac cagcccagcc gggagtcaca agccgaatac ctccatcccg
     4141 caggcgttct acaacaagca ggccggcatc aaacgtattg ccacggagac gggcgccgga
     4201 cagtggggta gcgcgctcgc gctcgcctgc cggctcttcg gcatggagtg tacggtctac
     4261 atggtgaaag tgagctacca gcaaaaaccg taccgccgga tgatgatgaa cacgtggggc
     4321 gccgaggtct acgccagccc cagcgagcac accaatgccg gccgccaggt gctcgcggcg
     4381 taccccgatt cgcccggcag cctcggcatc gccatcagtg aggccgtgga ggacgcggcc
     4441 actcatcctg atacgaacta ctcgctcggc agcgtgctga accacgttaa tctacaccag
     4501 accgtgatcg gccaggaggc caagctccag atggcgatgg ctggcgagga cccggacgtg
     4561 atcatcggtt gcttcggcgg cggaagcaac tttggcggta tcgcgttgcc ttacgtcctc
     4621 gacaaattca atggctctgc cgtgaggctg atggcggtcg aaccggcggc ttgtccgacg
     4681 ctaacaaagg gggccttcac ctacgacttt ggcgacatag ccaaactgac gcctctgctc
     4741 aggatgtaca cgctcgggca cgacttcgtg ccgccgggca tccacgcggg aggattgcgc
     4801 taccacggcg cgtccccact gttgagccac ctgatcaatc aggggtacat cgaagcgcgc
     4861 tccgtcctcc agattccgac gttcgaggcg gcagttctgt tttcccagac cgaaggattg
     4921 ctcccggctc ccgagtccgc acacgcgatc cgagcggcca tcgacgaggc gatcgcggcc
     4981 cgtgaggagg gccgcccccg cgtgatcctg ttcaacctga gcggccacgg ccacttcgac
     5041 ctggcctcgt acgaggccta cctcagtggc aagcttcagg actacgaata
//
LOCUS       DATIFQ010000531         6878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2102967, whole genome shotgun sequence.
ACCESSION   DATIFQ010000531 DATIFQ010000000
VERSION     DATIFQ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102967"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3234
                     /locus_tag="VLE22_26615"
     CDS             <1..3234
                     /locus_tag="VLE22_26615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB18053.1"
                     /translation="GFKPVTQKGVAVVINTVASIDITLEVGAVTEAITVEASAALLQT
                     TKTDVNVSLESRAIGNLPLPGYRNYQSLINLVPGATPTRYQNAVTDTPQRALTTNVNG
                     QERGANNTRVDGSADILVTMPHHTVYVPPVESIEEVNISTKNFDSEQGMTGGAAVTVT
                     TKSGTNEFHGSAFGMHNNSALRSLLWDENRAGVTKKPKGIRNIVGGSLGGPIKRNKLF
                     FFGDWEGTFERVNSARLFSVPTDDLRTGDFSKQLGAQILDASGNPISIPTTEGGSVPL
                     QEGMVFDPYTGDLDGTGRSVFSSGGRLNVIPSSRLNGPMMKVLDLVPHPNQPGDLSNY
                     FNSGTTRFNRNNVDAKVNWNRSDKHQLWFKYGVMTALVHGDFGLGDAGGECLCQGGGL
                     GDGQTLVQIGGIGQTYTVSPTFLIDGTLGWTRFGQSVKPPDLGTNFGSDVLGIPGTNG
                     PDPRESGMPAFYMDDYSALGNPEGWNPLFRNDQSYTANVNASWMKSSHEIRFGFDFVH
                     HLMNHWQPELGEGPRGAFYFYNAATALNPEALEGTVGFQGDTPSFENSWNGLAGFLLG
                     ASSQTGKSSQFIKMDSLENQYALYVRDRWRATSKLTLNLGLRWELYPNRRRSAGLGIE
                     SYDPTTNEVLVGGKGGIPRDSGVGYSKKLFAPRIGFAYQLTNSTVIRSGYGITYHSHP
                     WGAQALRGWYPLTIVAVFSGTNGYQPITTSPSYVEAGVPNKPLGSDVGIIPICCPDIS
                     QGRLPLPEVSEMGYPVANRELRRGYIQSWNFILEQKLPGQFVSSVGYVGTGSVNGFAF
                     LDINASQIPGSGDDGRPLFPEFGRTAATREWDGRTHSNYHSLQATINRRLAGGLLLKG
                     AYTYSHAIDQANYSDWTEFRWNAASVFHRNRASADHNIPHMFQLGYVYELPFGSTKQW
                     ATTGAANTILGGWQINGIFSAYQGRQYTLTASGASLNMPGNQQTPDQINPKVAKLGNV
                     GDDGTFFDTTAFARPTGARFGNVGRNTMRGPGVVNTDLSLFRTFKPMEKLSVQVRAEA
                     FNLSNTPHFSNPNGNRNSSNFGKVLSTQSADALGRSREFRFGLRLSF"
     gene            complement(3285..5006)
                     /locus_tag="VLE22_26620"
     CDS             complement(3285..5006)
                     /locus_tag="VLE22_26620"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB18054.1"
                     /translation="MRGRASRRDVLKTLAAAGLAGGVPLLGAQAGEPPLFRDVAAEVG
                     LDFHHFNGATGRHYMPEIMGAGVALFDYDNDGDLDIYLVQGTRLDSTGKLLSPPPPGW
                     RPGNRLFKNLLAETGELRFVDVTEKAGVGYIGYGMGVAAGDYDNDGFLDLYVTNFGHN
                     VLYHNNGNGTFTDVTHEAGVDDPRWSTSAAWLDIDGDGYLDLFVCNYVDFTVEGNRAC
                     SSPAGAPSYCTPKAYRAVPCRLFRNMRNGKFEDVSDASRISSSYGPALGVVCADFNGD
                     GRTDIFVANDTAANRLWLNQGDGTFRESALEMGVAYSMEGLAKAGMGVTLADVENSGA
                     QVLLVTNLTREGVTVHRGDTRGQFDDVTIPFGLLQPTFANTGFGTQWFDYDNDGWLDL
                     FIANGGVTILGSERSGVSPYAQRKQLFHNEGRGKRFRETSQSGGPVFQIDEISRAAAF
                     GDIDNDGAMDMVVTNNDGPVRLLRNQAGGRRHWLTVKLESPKTNRFGIGSRVAVIRRG
                     QEVLSRRVNTDSSYLSANDVRAHFGLGDQADIEAVLVHWPDGSEERFETIKADRIVTL
                     RQGSGKK"
     gene            complement(5003..6427)
                     /locus_tag="VLE22_26625"
     CDS             complement(5003..6427)
                     /locus_tag="VLE22_26625"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB18055.1"
                     /translation="MRVWFGAQIPVLVCALCVCAHPQDPAGGDLPELPKIDTADFLPV
                     IRAQIERAERNARTHSRDANAAGALGMTLHAYERYEVAARAYAHAQRLDPEAFDWVYL
                     SAVLLTQQGDFDGAVKSFRKALELRPGDLAAELRLGQSLSSTGNWDEAGTLYRKIVEA
                     HPDCPQAWYGLGRVQAAKGEHEAAAQSYSKACDVFPPYGAAHFALAGELRKLGKSTEA
                     EEHAAAYLKDPSAEPPLHDASLQRVRELNQGVHTHLQRGGAFEKEGKLEDAIREHEAA
                     LAIDPDNVQVHINLISLYGRTGDEARAKVHFEAATRLSPGRPDAWYNYGVLMSKRKEY
                     REAEGAFRRAVEINPYYAEAHNNLGALYELQGRLNDAAREFRDAIANKPDYPLARFHL
                     ARILVNEEKYDEAIQQLLRALTPEDDQTPVYLYALAAVYARAGDREHALNYYGRARDA
                     AHARGQVQLLGSIERDLKMFGGKR"
     gene            6571..>6878
                     /locus_tag="VLE22_26630"
     CDS             6571..>6878
                     /locus_tag="VLE22_26630"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSB18056.1"
                     /translation="MAGKSTVAAEECSALGAPDRTLESFPNSNVMLGNLPVARALPVR
                     GRRLVGSWCFLDRFGPLTFAGEKPMDVAPHPHIGLQTVTWLLDGEILHDDSLGCEAVA
                     R"
BASE COUNT         1327 a         2151 c         2068 g         1332 t
ORIGIN      
        1 gggttcaagc ctgtgaccca gaagggcgtc gccgtcgtca tcaataccgt cgccagcatt
       61 gacatcactt tggaggtggg tgcggtcacg gaagccatca ccgtggaggc cagcgccgct
      121 ctgctccaaa cgacgaagac ggacgtcaat gtgagcctgg aatcgcgggc gatcgggaac
      181 ctgccgctgc ccggctaccg aaactaccaa agcctcatta acttggttcc aggggcgaca
      241 ccaacgcggt accagaacgc cgtcaccgac acgccacagc gggccttgac aacgaacgtc
      301 aacggccagg agcgcggcgc caacaacact cgcgtggacg gttcggccga tattctggtc
      361 actatgcccc accacacagt ctacgtcccg ccggtggaaa gcattgagga agtcaacatc
      421 tccacgaaga acttcgattc cgaacagggc atgacaggtg gcgccgccgt caccgtgacg
      481 acgaagtccg ggaccaacga attccacggc agcgctttcg gcatgcacaa caacagcgcc
      541 cttcggtccc ttctgtggga tgagaatcgc gccggcgtaa ccaagaagcc caaaggcatc
      601 cgaaacatcg tggggggtag cctcggtgga cctatcaaga ggaataagtt gttcttcttc
      661 ggtgactggg aaggcacttt tgagcgcgta aacagcgccc ggctcttttc cgttccaacg
      721 gatgatctcc gcacgggtga cttcagcaaa caactcgggg cgcaaattct ggatgccagt
      781 ggcaatccca tctcaatacc tacgaccgag ggcggctccg tgccgctcca ggaagggatg
      841 gtctttgacc cctatacggg cgacttggat ggcacaggcc gttcggtgtt ttcgagcggt
      901 ggccgcctca acgtcattcc ttcttcccgg ctgaacggcc ccatgatgaa agtgctggac
      961 ctggttcccc accctaacca acccggggat ctgagcaact acttcaactc cggcacgacg
     1021 cgctttaacc gtaacaacgt tgacgccaag gtcaattgga accggagtga caagcaccaa
     1081 ctctggttta agtacggcgt catgaccgcc ctggttcacg gtgacttcgg cttgggcgac
     1141 gcagggggcg agtgtttgtg tcaaggtggc ggcctcggcg acggccagac gctcgtccaa
     1201 atagggggca tcgggcaaac ctataccgtt tcaccgacat tcttgattga cgggacgctc
     1261 ggctggaccc gatttggcca gagtgtgaag ccgcccgacc tcggcaccaa cttcgggtcg
     1321 gacgtgctgg ggattccggg gacgaacggt ccagacccgc gggaaagcgg catgcccgct
     1381 ttttacatgg acgactactc ggcccttggc aacccggaag gatggaaccc cctgttccgg
     1441 aatgaccagt cctacacggc gaacgtcaat gcgagctgga tgaagagctc ccacgagatc
     1501 cggtttggct tcgactttgt ccaccatctg atgaaccact ggcagccgga gttgggagaa
     1561 ggcccgcgcg gcgcattcta tttctataat gccgctacgg ccctcaatcc cgaagctctc
     1621 gagggcacgg tgggatttca gggagacact ccatcgtttg agaacagttg gaacgggctg
     1681 gcggggtttc tacttggcgc gtccagtcag acggggaaga gtagccagtt catcaagatg
     1741 gacagcctgg agaaccagta cgctctctac gtccgcgacc gctggcgcgc cacgtccaag
     1801 ctgacactga acctgggctt gcggtgggag ttgtacccca accggaggcg ctccgccggc
     1861 ttgggtatcg aatcgtacga ccccaccacc aacgaagtcc tggttggcgg gaagggtggt
     1921 atccccagag acagcggagt gggctacagc aagaagctct ttgcaccccg catcggtttc
     1981 gcgtatcaac tgacgaattc cacggtgatc cgaagcgggt acggcatcac ctaccactcg
     2041 catccctggg gtgcgcaagc gttgcgtggt tggtacccgc tgacgattgt tgcggtcttc
     2101 tccggcacca acggctacca acccatcacc acgagcccca gttacgtgga ggcgggcgtc
     2161 cccaacaaac cgctcggctc cgacgttggc attattccga tctgctgccc ggacatcagc
     2221 caggggaggc ttccgctgcc cgaggtcagc gaaatgggat acccggtggc caaccgggag
     2281 ttgcgccggg gctacatcca gtcgtggaac ttcatcctcg agcagaagct gccgggccag
     2341 ttcgtgtcgt ccgttggata cgtcgggaca ggatccgtga acggcttcgc gtttctggac
     2401 atcaatgcct cgcagatacc cggttccggg gatgacgggc ggccgctctt cccagagttc
     2461 ggacgcacgg cggcaaccag ggagtgggac ggccgtacgc acagcaacta tcactcgctg
     2521 caagccacca tcaaccggcg tctcgcgggc ggtctgctcc tcaaaggcgc ttacacctac
     2581 tcgcacgcta tcgatcaggc taactactct gactggacgg agtttcgctg gaacgcggcg
     2641 agtgtatttc atcgtaaccg cgcctcggcg gaccacaaca tcccccacat gttccagctt
     2701 ggctatgtgt atgagctccc cttcggcagc accaagcagt gggctacaac cggcgcggcc
     2761 aacacgatcc tgggcggctg gcagatcaac ggcatcttta gtgcctacca ggggcggcag
     2821 tacacgctca ccgcttccgg ggcgtcgttg aacatgccgg gaaaccagca gaccccggac
     2881 cagatcaatc cgaaagtggc caagctgggc aatgtgggcg atgatgggac ctttttcgac
     2941 accaccgcat tcgcacggcc cacgggggcc cgcttcggca atgtgggccg caacacgatg
     3001 cgtggccccg gtgtggtaaa tacggaccta agcctctttc gcaccttcaa gccaatggaa
     3061 aagctcagcg tgcaagtccg ggcggaggcg ttcaacttga gcaacacgcc gcacttcagc
     3121 aaccccaacg gcaatcgcaa cagctcgaac ttcggcaagg tcctctccac gcagtccgcg
     3181 gatgcactcg ggcgctcccg tgagttccgc ttcggcttgc gtctgagctt ctagcctgaa
     3241 tgatcgcctg tcagccgggc gcgggcattg cagtccgcgc ccgactactt tttccccgat
     3301 ccctggcgaa gcgtcacgat gcggtcggcc ttgatggttt caaaccgttc ttcggagcca
     3361 tcgggccagt ggaccagcac cgcttcgatg tctgcctgat ctcccagtcc gaagtgagcg
     3421 cgcacatcgt tcgcactgag atagctggaa tcggtgttta cgcgccgcga caaaacctct
     3481 tgaccgcgcc ggatcaccgc gacgcgtgaa ccgatgccga accggttcgt cttcggcgac
     3541 tctagcttca ccgtgagcca gtgcctccgg ccccctgcct ggttgcgaag cagccgcacg
     3601 gggccgtcgt tgttcgtgac aaccatgtcc atcgcgccgt cattgtcgat atctccgaag
     3661 gccgccgcac ggctgatttc gtcaatctga aataccgggc ccccggactg cgacgtctcg
     3721 cggaaacgct ttccgcggcc ctcgttatgg aatagctgct tccgctgagc atagggagag
     3781 actccactgc gctcggatcc caagatcgtg acgccgccat tggcaatgaa tagatcgagc
     3841 cagccgtcgt tgtcataatc gaaccattgc gtaccgaagc cggtgttggc gaacgtcggt
     3901 tgcagcaggc cgaacgggat ggtcacatcg tcaaactggc cccgcgtgtc gccgcggtgc
     3961 actgtcacgc cttcgcgcgt caggttcgtt acgagcaaca cctgggcgcc gctgttctcg
     4021 acgtcggcga gtgtcactcc catccctgcc ttggccagcc cctccatgct gtaagcaacg
     4081 cccatttcca gcgccgattc acggaacgtg ccgtcgccct gattcagcca cagccgattg
     4141 gccgcggtgt cgttcgcgac gaaaatatca gtgcggccgt caccgttgaa gtcggcgcaa
     4201 acgacgccta gcgcgggtcc ataagaactg ctgatgcgcg acgcgtccga gacgtcctcg
     4261 aatttgccgt tcctcatgtt tcgaaacagg cggcacggca ctgcgcgata tgccttgggc
     4321 gtgcagtagc taggcgcacc ggccggcgaa gagcaggcac ggttcccctc cacggtgaaa
     4381 tcgacgtagt tgcacacgaa cagatcgaga taaccgtcgc catcgatgtc gagccaggcg
     4441 gcgctcgtcg accagcgcgg atcgtcgacg cccgcctcgt gggtaacgtc ggtaaacgtg
     4501 ccgttgccgt tgttgtgata gagcacattg tggccgaagt tcgtgacgta gagatcgagg
     4561 aaaccgtcgt tgtcgtagtc tccggctgcg acacccatcc cgtatcctat gtagcctacg
     4621 cccgccttct cggtcacatc cacaaaccgt aattcacccg tttcggccag cagattcttg
     4681 aacaaccggt tgccgggcct ccagcccggt gggggcggcg agagcagctt cccggttgag
     4741 tccagtcgcg tgccctggac aagataaata tcgaggtcgc cgtcgttgtc gtagtcgaaa
     4801 agggccacgc cggcgcccat aatttcgggc atgtaatggc gccccgtggc gccattgaaa
     4861 tgatggaagt cgaggccaac ttcagccgct acatcgcgga aaagcggcgg ctcgccggct
     4921 tgggcgccca acaggggaac acccccggca aggcccgcgg ccgcaagcgt tttgaggacg
     4981 tcgcgacggg aagcgcgtcc tctcatcgct taccgccaaa catcttcaga tcgcgctcga
     5041 tgctgccgag cagttgtacc tggccgcgcg catgagccgc atcgcgtgct cgcccgtagt
     5101 agttgagtgc gtgttcccga tcgccggcgc gtgcgtagac ggccgcgagt gcatacagat
     5161 agaccggcgt ctgatcgtct tccggcgtta gtgctctcag taactgctgt atcgcctcgt
     5221 cgtacttctc ctcattgacc agaatgcggg cgaggtggaa gcgcgccagc ggataatcgg
     5281 gcttgttcgc gatcgcgtcg cggaactccc tggctgcatc gttcagccgc ccctgcaatt
     5341 catagagtgc tccgaggttg ttgtgcgcct cggcgtagta ggggttaatc tcgacagcgc
     5401 gacggaaagc cccttccgct tcgcgatact ctttgcgctt tgacatcaga acgccatagt
     5461 tgtaccaggc atccggccgg cctggactta gccgcgttgc ggcctcgaaa tgcacttttg
     5521 cccgcgcctc gtcgcccgtg cgtccataga gcgagatcag gttgatgtgg acctggacat
     5581 tatcgggatc gatggccaga gctgcttcgt gctcgcggat ggcatcctcg agcttgcctt
     5641 ccttctcgaa ggccccgcca cgctgcaggt gagtgtgtac gccttggttc agttcgcgaa
     5701 cgcgctggag cgacgcgtca tgaagcggcg gttcggccga ggggtctttc agatatgcag
     5761 cggcatgctc ttccgcttcc gtgctcttcc ctagcttccg gagctcgccg gccagcgcga
     5821 aatgtgcggc gccatacggc ggaaacacat cacaggcctt gctataggac tgggctgccg
     5881 cttcgtgctc gcccttcgct gcttgcacgc ggcccaggcc gtaccaggcc tgaggacagt
     5941 caggatgcgc ttcgacgatt ttccggtaca gcgtgcctgc ttcgtcccaa ttgccggttg
     6001 aacttaagct ctggccgagg cgcagttccg cggcaagatc gccgggccgg agttcgagcg
     6061 ccttccggaa cgacttcacg gcgccatcga agtcgccctg ctgcgtgagc aggacagcgc
     6121 tcagatagac ccagtcgaaa gcctctgggt ccaatcgttg cgcgtgcgcg taggcgcgcg
     6181 ccgccacctc gtaacgctcg taagcatgaa gagtcatccc taatgcgccg gctgcgtttg
     6241 cgtcgcgaga atgggtcctg gcatttcgtt cggcgcgctc aatctgagct cggatgacag
     6301 gaaggaaatc ggcagtgtca atctttggta gttccggcag gtctccgccg gcggggtcct
     6361 gtggatgcgc gcacacgcac agggcacaca ccagcacggg gatttgggcg ccaaaccaga
     6421 cccgcataca accgtgcttt ttattgtagc cgcactagac tcaggttcga gtaagcttcg
     6481 ggtcactcaa cagctccggt aaccgtcatc cgttgctatt ctgggcctag gtcgtcagac
     6541 gcggagcctg gaaacgagag gagttcacgc atggctggga aatccactgt cgcggcggaa
     6601 gagtgtagcg cgctcggggc gccggatcgg acgctcgagt cgttcccaaa cagcaatgtc
     6661 atgcttggca acctcccggt tgcgcgtgcg ctgccggtca gaggacggcg gctcgtgggt
     6721 tcatggtgct tcctcgaccg gtttggaccg ctgaccttcg cgggcgagaa gccaatggac
     6781 gtggcgcccc atccgcacat cggccttcaa acggtgacgt ggctgctcga cggcgagatc
     6841 ctgcacgacg acagcctcgg gtgtgaagcg gtggcgcg
//
LOCUS       DATIFQ010000532        11347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_760338, whole genome shotgun sequence.
ACCESSION   DATIFQ010000532 DATIFQ010000000
VERSION     DATIFQ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11347
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760338"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VLE22_26635"
     CDS             complement(<1..458)
                     /locus_tag="VLE22_26635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18057.1"
                     /translation="MPPIVAVASVWRDGYFESAMTGKERIALTMRHQEPDRVPVMCQL
                     SIGHYNLNGGYKPHEIWYETEAFAEASEKLARRYAFDGILVVLTGRPPNYLDAAVSIK
                     AERDGEWVTWRNGDRTFMPWDDMPHHYPADTSKPARADFEAFNPDRDLNRV"
     gene            457..1353
                     /locus_tag="VLE22_26640"
     CDS             457..1353
                     /locus_tag="VLE22_26640"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HSB18058.1"
                     /translation="MGPFCVRGVLLVLLAASVGPIRGSDGVSEHMLVSTAWLAGHLAD
                     PSVVILHVGSQEDYAAGHIPGARLLRLADISVTGDGGLTLELPSVSLLQQAFGKVGIS
                     HDSRVIVYPATESVQAATRVWFTLDYLGLGDRASLLDGGLQAWRASGNPLSTDTPVVE
                     AAEVKTLARQGRVVDAEWVQAHLRDTEVALLDARAAEFYTGENAGRMPRAGHIPGARN
                     LPFTSLFDENRKLLSADSLRELMGARGVTQGKLMVSYCHIGQQATVLYFVARYLGFDA
                     RLYDGSFQDWSNRLAFPIETGP"
     gene            complement(1362..2525)
                     /locus_tag="VLE22_26645"
     CDS             complement(1362..2525)
                     /locus_tag="VLE22_26645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18059.1"
                     /translation="MARNVAVDAYRGFVMFLMMAEVLRLARVAQALPGNWFWGFLAYN
                     QTHVAWAGCSLHDLIQPSFSFLVGVALPYSIASRMAKGGTFGKMFGHAVWRALLLIAL
                     GIFLRSMHSSQTNFTFEDTLTQIGLGYPFLFLLGFRPARWQWGALAAILTGYWLAWAL
                     YPVPGPGFDYQAVGVPPDWQHHYSGFAAHWNKNSNLGLAFDQWFLNLFPRTKPFIANF
                     GGYLTLSFIPTLGTMILGLAAGRWLRAAAPRIPMKQLLIAGAAGIAAGVVLHVAGICP
                     VVKRIWTPSWTLFSGGACFLLLAGFCWVIEAKGYKKWAFPLVVIGVNSIASYLIAHLF
                     DDFFFNSFRIHLGPEPFRVFGPAFEPFLQGAAVLLMYWLVLFWMYRRKLFLKI"
     gene            complement(2680..3081)
                     /locus_tag="VLE22_26650"
     CDS             complement(2680..3081)
                     /locus_tag="VLE22_26650"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSB18060.1"
                     /translation="MRFRQRVAALVAVLMAACALSAKAPEPRGSAKVVSDADLERAIR
                     ERLARSKCAADNFTVKVQGGVATIEGKTNVMQRKGAATRMARSAGAKKVVNRIQISDA
                     ARQKASERLAKGRRRVQVKRGEARETSPGRE"
     gene            3128..4336
                     /locus_tag="VLE22_26655"
     CDS             3128..4336
                     /locus_tag="VLE22_26655"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB18061.1"
                     /translation="MSETILGRRAFLRGAGTAALTAASYSRVLGANDRIAMGVIGPGD
                     RGRYVMGLFQNNASIDVVGLADVYAVQIDLAKLKAPNARSFNDYRKLLEMKEVDIVLI
                     ATPDHWHSRTAIDALNAGKDVYVEKPLTLTIQEGPEIVKAARVNERICQVGMQQRSGA
                     HYLEAKREYFDTGRLGKITLARTWWHGNSYHLRTAPESLRTRPSNLDWARFLGPLKWR
                     DYDPQQYWNWRAYLDFGGGQITDLFTHWIDVVHMLMGRDVPTAAVASGGVYCYKDGRT
                     APDTINVLLEYGGEFTATFEATLVPGITGEAVEIAGTEGRLWIDRSRYEFHPAGKNAQ
                     PVIVKASETDMTQDHVNNFLECIRSRRLPNGDVLIGHRSAQASHLGNIAYLQKRRLAF
                     DPIREEILPL"
     gene            4548..6215
                     /locus_tag="VLE22_26660"
     CDS             4548..6215
                     /locus_tag="VLE22_26660"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB18062.1"
                     /translation="MRIAVSALALTFAAAIRLAPLGPPEPPVTFQNVADSSGVDFVLD
                     NFATPKKHQIETMISGVAAFDFDNDGLLDLYFVNGARIPELDKSAPRFWNRLYRNKGG
                     ARFEDVTERAGVKGIGYGMGVAAADYDNDGFVDLYVAGVNRNQLLHNNGDGTFTDLTE
                     KAGVSGTHPKFGKTWAISAGWFDYDNDGHLDLLVVSYVNWSPEIEPECRSAGIRAYCS
                     PNSYSGQPNILYHNNGDGTFTDVSDASGIGRHIGKGMAVAFADVDADGFTDAFVSNDT
                     YRNFFFHNNGDGTFRETALRMGVAYNENGKSIAGMGADFRDLDNDGRPDIFVVAMVGD
                     TFPLFLNRGKDFVDITSRSGLARLCAGFTAWSNGIADFDNDGFKDLFTTNSSILDNAE
                     EIERLPSKLPNSIFRNLGDRTFADVSTTAGPTFRVPQSHRGAAIGDLDNDGRLDLVIT
                     SPQARPEIFLNRTANRNHWLSVKLVGVKSNRDGLGARVKVTLQGGKALYNHATTSVGL
                     SSSSDKRVHFGLGAETALESVEVLWPSGIRQTLTNLKTDQILEIREK"
     gene            complement(6243..8069)
                     /locus_tag="VLE22_26665"
     CDS             complement(6243..8069)
                     /locus_tag="VLE22_26665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18063.1"
                     /translation="MTDPKLVVRHSELADGPIVRDGEWTMRFTLPRRFGFDFHWLREV
                     ARGAIEGDLGLRVDANAGVALDMALAGSFRQVVSLDAEQRLRLQVIKRNADGIRLGAG
                     VTAGVRMETPLPEGADELTAAILGAHRAQAAAGYAQILAPGALPSVLGLRDEIYRRAM
                     EALGRRYTVEATVQFERTEEGASLFDCSFAFSEEGLRLYRAALDGDFSEILTRESRDV
                     QVREAAVARALRERTHIELHLPFFDRKQWEARVEAFAAMHVESDDSGRLLVYHAGGTG
                     TLVLKNAYQSTLALAGGLSVGRVHSEANFTLSYTDRRRMPTASAASRLEDVLSAYEFE
                     SGAAEWLARMGEERTGAELESTLTLSVPGEVVSAWLGAPDERSDVYFRTYARVSRAVQ
                     RAMRRWLPLVYFREAERYEALGAAFPLLVYQCSKIYECRPKHDFSYDVMSREWREEFF
                     SRAEKRLRKELPRVHRLLETEGRKRAARYYSPRQAEDIAVSVARSPRLLISLLWADAL
                     FVDSLITLGCRAGTLRAVMAKDPQRAVRQLTDFSSELVKAFHSRLRRLYGGEDFVALG
                     SLLLVEATNALRTEFRETTAIRGTLTVKADGVERTFVNAAWR"
     gene            complement(8195..8443)
                     /locus_tag="VLE22_26670"
     CDS             complement(8195..8443)
                     /locus_tag="VLE22_26670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18064.1"
                     /translation="MGFALWVDSEMAWAQGRHEYQPMGAAVVARSDLFRERDFRRTRR
                     SPSYYSDSYVGLFASLGDVNAYLEGMRLTERAVHKPAK"
     gene            8472..9296
                     /locus_tag="VLE22_26675"
     CDS             8472..9296
                     /locus_tag="VLE22_26675"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HSB18065.1"
                     /translation="MTRRSFFPTAAMIPALAAGAAPLKGRLKQSATRSCFKGMPIEDA
                     AREAAQLGLKGLDLIGSNDWPVLKKYGLISTMAPGGGTIADGINRRENHAKIEQQLHE
                     NVQKAHDFGLPNVITLSGNRRGMADEEGIDNSVLLLNKIKGRAEDQGVTVCLELLNSK
                     VDHPDYHCDHTRWGVEVCKRVNSPRVKLLYDIYHMQIMEGDIIRTIRDNIQWIGHFHT
                     AGNPGRHEIDASQELNYRAIAQAIVDLGYTGYFAHEYSPLRDPLKSLAEAIEICDV"
     gene            complement(9301..>11347)
                     /locus_tag="VLE22_26680"
     CDS             complement(9301..>11347)
                     /locus_tag="VLE22_26680"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HSB18066.1"
                     /translation="RVLTWFARSYLQFYRLAGENPSVDLLRRSVSQFLARYRRANYPV
                     DAVLLYGLYTDNADIGHGDVETIRQWNEAFEYPKIIAATDGDYYDYLSRNFAGKLPVY
                     RGDAGAYWEDGAGSTALETTMNRDSQRMLPVAEMAAALASAFRPDEVYPAAEFRDAWK
                     NLLFYDEHTWGAHSSVNQPGRRFVTAQWEHKRAYAWRAHWATTDLLYRSFNRLVQNIS
                     VDGPTLYVFNPDLWPRSDMVTAEIEPSREIVDMVTGKPVAVDVVSETDGWRKLRFLAE
                     NVPGLGYRAYAVRNIAGKVPSGTEAQPQEWQLESTFYRLTLDPATGAVAGLLDKETGR
                     DLVDTAAPYKLNQLVYVTGGERSRMLQDLATLPAPRLDLSGPRNASIVESVRTPYGKR
                     IRVHAEAKNVPVIDTEISLYDGIKRVDIVNRLEKRETRDKEAVYFAFPFRVSPPQLAY
                     QVQNAWVRPNEDQLPGACRDWFTTQNLVRAGDAGITIAWATPDAPLITLADINRGLWL
                     KHLTVNNGHVLSYAMNNYWFTNYKAAQGGEFTFRYAITSGRDLGDDALARFDQETRSP
                     LFAYAVYDLGNNRLKPAKRVMAAASGSFLTIEPTHAQVTAFKEAEDGDGFILRLRETA
                     GKPGTAHVTSPVFPLLAATLTNGVEDGATPAAVSANRVELPLKPYGFTTVRLKFGKP"
BASE COUNT         2142 a         3792 c         3326 g         2087 t
ORIGIN      
        1 accctattga ggtcgcgatc aggattgaaa gcttcgaagt cagcgcgcgc cggcttcgag
       61 gtatccgccg ggtaatggtg cggcatgtcg tcccagggca tgaacgtgcg atcaccgttg
      121 cgccacgtga cccattcacc gtcgcgctcg gccttgatcg aaacagccgc gtcgagataa
      181 ttcggcggcc gccccgttag taccaccagg atgccgtcga acgcgtaccg ccgcgccagt
      241 ttctcgctgg cctcggcaaa cgcctcggtc tcgtaccaga tctcgtgtgg cttgtatccg
      301 ccgttcagat tgtagtggcc gatcgaaagc tggcacatca ccggtacacg atccggttcc
      361 tggtgccgca tcgtcagcgc gattcgttcc tttccggtca tcgcgctctc aaaataccca
      421 tcccgccaga ccgacgcaac tgcaacaata gggggcatgg gtccgttctg cgttcgaggg
      481 gttcttctgg tcctcctggc ggcatccgtc ggccctattc ggggttcgga cggcgtcagt
      541 gagcacatgc tcgtctcgac cgcgtggctc gccggtcatc tcgccgaccc gtccgtcgtc
      601 atcctccatg tcggctcgca ggaagactac gccgccggcc acattcccgg tgcgcgtctt
      661 ctccggctgg ccgacatctc cgtcaccgga gacggcggcc tgacactcga actgccgtcg
      721 gtgagtcttc ttcagcaggc cttcggcaaa gttggcatct cccacgactc tcgcgtgatc
      781 gtctaccccg cgacggagtc cgtgcaggcg gcgactcgcg tgtggttcac gctggactac
      841 ctcggcttgg gtgatcgagc gtcgctcctc gatggcggtc tccaggcatg gcgcgcttcc
      901 ggcaatccgc tgagcacgga tacgccggtg gtcgaagctg ccgaggtcaa gacgctggct
      961 cgacaagggc gggtggtcga cgccgaatgg gtccaggcgc acctgcgcga cacggaagtt
     1021 gcgctgctcg acgcccgtgc ggcggagttt tataccggcg agaacgccgg aaggatgccc
     1081 cgcgccgggc acataccggg cgcgcgaaat ctccctttca ccagcctgtt tgatgagaac
     1141 cgtaagctgc tgtcggccga ctcgctgcga gagctcatgg gcgcccgcgg cgtaacgcag
     1201 ggcaagctca tggtgtccta ctgccacatc ggccagcagg ccacggttct ctacttcgtc
     1261 gcccgctacc ttggcttcga cgcccgcctc tacgacggct ccttccagga ctggagcaac
     1321 cggcttgcct tccccatcga gaccggaccg tagggccgcg ttcagatttt caggaacagc
     1381 ttccggcggt acatccagaa caggaccagc cagtacatca acaacaccgc cgcaccctgt
     1441 agaaaaggct cgaacgctgg cccgaagact cggaacggtt caggccccaa gtgaatgcgg
     1501 aatgaattga agaaaaagtc gtcgaacagg tgggcgatca ggtaggacgc gatcgagttc
     1561 acgccgatca ccaccagcgg aaaggcccac ttcttgtatc ccttcgcttc aattacccag
     1621 cagaacccgg ccaataacag aaagcaggcc ccaccgctga aaagtgtcca gctcggcgtc
     1681 cagatgcgtt tcaccaccgg gcagatcccg gcgacgtgca gcacaacacc cgcggcgata
     1741 cctgccgcac ccgcgatgag aagctgcttc atcggaattc ggggcgccgc cgcacgcagc
     1801 cagcgtcctg cggctagccc cagaatcatt gtgccgagcg ttggaatgaa gctgagcgtg
     1861 aggtaccctc caaaattggc gataaacggt ttcgtcctag ggaacaggtt caaaaaccat
     1921 tgatcgaacg caagcccaag attactgttc ttgttccagt gcgccgcgaa acccgagtag
     1981 tgatgctgcc agtccggcgg cactcccacg gcctgatagt cgaagccggg acccggcacc
     2041 ggatacagcg cccaggcaag ccagtagccc gtcaggatcg ccgcgagcgc cccccattgc
     2101 cagcgtgccg gccggaaccc gagcaagaac agaaacggat agcccagccc gatctgcgtc
     2161 agtgtgtcct cgaacgtgaa gttggtctgc gagctgtgca tggaacgcag gaagattccc
     2221 agcgcgatca gcagcagagc tctccacacc gcgtgcccga acatcttgcc gaacgtcccg
     2281 cccttcgcca tccggcttgc aatcgaatag ggcagcgcca cgccgaccag aaaagaaaat
     2341 gatggctgga tcagatcgtg cagtgagcat ccggcccacg cgacatgcgt ctggttatag
     2401 gcaaggaagc cccaaaacca gtttccgggg agggcctgtg ccacccgcgc caggcgcagc
     2461 acctccgcca tcatcagaaa catcacgaat ccgcggtacg cgtccaccgc gacattgcgc
     2521 gccactggct gtgcactcgg ctgcaagcga taccccccga gcgatcaaca ctaacacaga
     2581 tgcgcgctca tgcggagtca ggtacaggat tgcagcgctc ggaacccatt tcgcgtcgat
     2641 cggtttgcat tcgcggcttg actccgccaa gggttcgctt cactcccgcc ccggactggt
     2701 ctccctcgcc tcccctctct tcacctgcac cctccgccgg ccctttgcca accgctccga
     2761 tgccttctgg cgcgccgcgt cgctgatctg aatccggttc accaccttct tggcccccgc
     2821 cgacctggcc atccgcgtcg ccgcgccctt ccgctgcatc acgttggtct tgccctcgat
     2881 cgtcgctact ccgccctgca ctttgaccgt gaagttgtcc gccgcgcact tcgagcgagc
     2941 cagtctctcg cgaatcgccc tctccaggtc cgcgtcagat accaccttcg cggacccgcg
     3001 tggctccggc gcctttgcgg atagcgcgca tgccgccatc agcaccgcca ccagcgctgc
     3061 tacacgttgt cggaatctca atttcgctct ccatgaacta agtgcgttcg ggagctataa
     3121 ttcgcacatg tctgagacga ttttggggcg ccgggccttc ctccgtggcg ccggcactgc
     3181 cgcgctgacc gccgcctcct actcacgcgt cctcggcgcg aatgaccgca tcgccatggg
     3241 cgtcatcggt cccggcgacc gcggccgcta cgtcatgggt ctcttccaaa acaatgcgtc
     3301 gattgacgtc gtcgggctcg cggacgtgta cgccgtccag atcgaccttg ccaagctgaa
     3361 agcgcccaac gcccgttcct tcaatgacta ccgcaagttg ctggaaatga aggaggtcga
     3421 catcgttctt atagccacac cggatcactg gcactcccgc accgccatcg atgcgctcaa
     3481 cgctggcaag gacgtctacg ttgagaagcc ccttacccta accatccagg agggcccgga
     3541 gatcgtcaag gccgcccgtg tcaacgagcg catctgccag gtcggaatgc aacagcgctc
     3601 cggtgcacac taccttgagg ccaagcggga atacttcgac acgggccgtc tcggtaagat
     3661 caccctcgcc cgcacctggt ggcatggaaa tagctaccac cttcgcacgg ctcccgagtc
     3721 cctccggact cggccttcca acctcgattg ggcccggttt ctcggtccgc tcaagtggcg
     3781 cgactacgac cctcagcaat attggaactg gcgcgcctac ctcgacttcg gaggcggcca
     3841 gatcaccgat ctgttcaccc actggatcga cgtcgtccac atgctgatgg ggcgggacgt
     3901 tcccaccgcc gctgtcgcgt ctggcggcgt ctactgctac aaagacggcc gcaccgcgcc
     3961 cgacaccatc aacgtcctgc ttgaatacgg cggcgagttc accgccactt tcgaggctac
     4021 cctcgtcccc ggcataacag gcgaggcggt cgaaatcgct ggcaccgagg gacgtctctg
     4081 gatcgaccga agccgctacg aattccaccc cgccggaaag aatgcgcagc cggtcatcgt
     4141 gaaggcctct gagaccgata tgacccagga ccacgtgaac aacttcctcg aatgcatccg
     4201 ttcccggagg ttgcccaacg gcgacgtcct catcggtcac cgctccgccc aggcctcgca
     4261 cctcggcaac atcgcctacc tccaaaaacg ccgcctggcc ttcgacccaa tccgcgagga
     4321 gatcctcccg ctctagccca ggagacaact actatgccgc gttgtgtctt cgtgtctccg
     4381 tggtgtctcc agcgttcctc gcggttggtc tggagggtta tgctaccttg ctagtgggtt
     4441 cggaatctgt tcgctctttg tcttgaagcc gtttgactct acggtgggca ggaaaaccgg
     4501 atcagatcct gaaactggag gagccccggc tgtaggtaag ggcaaccatg cggatcgctg
     4561 tctcggccct ggcgctcacc tttgcggccg ccattcgcct cgctccgctc ggacccccgg
     4621 aacctccggt gacgttccag aacgtcgcgg acagctccgg agtcgacttc gtcctggata
     4681 atttcgccac gcccaaaaag caccagatcg agaccatgat ctcgggcgtc gcggcgttcg
     4741 actttgacaa cgatggtttg ctggatctct atttcgtaaa cggtgctcgc atacccgagt
     4801 tggacaaatc cgctccccgc ttctggaacc ggctttaccg gaataagggc ggcgcgcgct
     4861 tcgaagatgt gaccgagcgc gcgggggtca aagggattgg ctacgggatg ggggtcgccg
     4921 ccgcggatta cgataacgat ggcttcgtag acctctacgt ggctggcgtc aaccggaatc
     4981 agcttctcca caacaacggc gacggcacgt tcaccgacct caccgaaaag gcgggtgtct
     5041 ccggcaccca cccgaagttc ggtaagacct gggcgattag cgccggctgg ttcgactacg
     5101 acaacgacgg ccacctcgat cttctggtcg tcagttacgt caactggagc cccgagattg
     5161 aaccggaatg ccgctcggcc ggaatccgag cctactgctc accaaacagt tacagcggcc
     5221 agccgaatat cctctatcac aataacggcg atgggacttt tactgacgtc tccgacgctt
     5281 ccggaatcgg ccgccacatc ggcaagggga tggccgtcgc cttcgccgat gtggatgcgg
     5341 acggattcac cgatgccttc gtttcgaatg acacctaccg caactttttt tttcataaca
     5401 acggggatgg cacgttccgc gaaacggctc tccggatggg agtcgcctat aacgaaaacg
     5461 gaaagtccat cgcgggcatg ggcgccgact ttcgagacct cgataacgac ggccgcccgg
     5521 atatctttgt ggtggccatg gtcggtgaca catttcccct ctttctgaac cgggggaagg
     5581 acttcgttga catcacaagc cgcagcggtc ttgcccgcct gtgcgcgggt ttcaccgcct
     5641 ggagtaacgg gatcgccgat ttcgacaacg acggcttcaa ggacctcttc acgacgaact
     5701 cgtccatcct cgacaatgcc gaagagatcg aacgccttcc ctccaagttg ccgaattcca
     5761 tctttcggaa cctgggcgac aggacgttcg ccgatgtgag taccactgcc ggcccgacgt
     5821 ttcgtgtacc ccagtcgcat cgcggcgccg cgataggcga cctcgacaac gacggacgcc
     5881 tggacctcgt catcacaagt ccgcaagccc ggccggagat cttcctcaac cggactgcga
     5941 accggaatca ctggctgtcc gtaaagctcg tgggcgtcaa gagcaacagg gacggcctcg
     6001 gcgcgagggt caaagtcacg cttcagggtg gaaaagcgct ctacaaccac gccacgacca
     6061 gtgtcgggtt gagttcctct tccgacaagc gcgtgcattt tggtttaggc gcggaaacgg
     6121 ctctggagtc cgtggaagta ctctggccga gcggtattcg acagactcta accaacctga
     6181 agactgatca gatcctggag atccgggaga agtaggcgag acgggcttgt ccccctctcc
     6241 aatcaccgcc acgctgcgtt cacgaacgtc cgctcgacac catccgcctt aacggtcagc
     6301 gttcccctaa tggcggtggt ctctcgaaac tccgtccgta gcgcgttggt cgcctccacc
     6361 agcagtaggg aacccagcgc gacgaagtct tcgccgccat acaaccgtcg cagccgcgag
     6421 tggaacgcct tcaccagctc ggagctgaag tctgtgagtt gtcgcaccgc gcgctgcgga
     6481 tccttcgcca tcactgcgcg caacgtcccc gccctgcatc ccaacgtgat caacgagtcc
     6541 acaaagagtg cgtccgccca cagcaggctg atcagcaatc gcgggctccg cgccaccgac
     6601 accgcgatgt cctccgcctg ccgcggactg tagtaccggg ccgcccgctt ccggccctcc
     6661 gtctccagca accggtgcac gcggggcagt tcctttctca atcgcttctc cgcgcgcgag
     6721 aagaactcct ctctccactc ccggctcatc acgtcgtagc tgaagtcgtg cttcggccgg
     6781 cactcataga ttttgctgca ctggtacacc agcagtggaa acgccgcccc cagcgcttca
     6841 taccgctccg cttcccggaa gtagaccaga ggcagccagc gccgcatcgc tctctgcacc
     6901 gcccgtgaca cccgcgcgta agtgcgaaag tacacatccg acctctcatc cggcgcgccc
     6961 agccacgcag agaccacctc tcccggtacg gagagtgtca gcgtgctctc caactccgcc
     7021 cccgtccgtt cttcgcccat ccgcgccagc cactcggccg ccccgctctc aaattcgtac
     7081 gcgctcaaga cgtcctcgag gcggctcgcc gcgctggccg tcggcatccg ccttcggtcc
     7141 gtgtaggaca gtgtgaagtt cgcctcggag tgcaccctgc cgaccgacag cccgcctgcc
     7201 agcgccagcg tgctttggta ggcgttcttg agcaccagcg tgcccgtccc gccggcgtgg
     7261 tagaccagca gccttcctga gtcatcactt tccacgtgca tcgccgcaaa cgcctccact
     7321 cgcgcttccc actgcttgcg gtcgaagaac ggcagatgaa gctcgatgtg cgttcgttcg
     7381 cgaagagccc gcgcaaccgc cgcctcgcgt acctgcacat cccggctctc ccgcgtgagg
     7441 atctcggaga aatccccgtc cagcgccgcc cggtacagcc gtaacccctc ctcgctgaac
     7501 gcgaacgagc agtcgaatag gctcgcccct tcctcagtcc gttcaaactg caccgtggcc
     7561 tccacggtgt acctgcgtcc cagcgcctcc atcgcccggc ggtagatctc gtcccgaagt
     7621 ccgagcacgg atggaagcgc cccgggcgcc agaatctgcg cgtagccggc cgccgcctgc
     7681 gcgcggtgcg caccgaggat tgcggccgtg agttcatctg cgccctccgg tagcggcgtc
     7741 tccatccgga cacccgctgt gactccggcg ccgagccgga tcccatccgc gttccgcttg
     7801 atcacttgaa gccgcagcct ctgctccgcg tcgagcgaga ccacctgccg gaaactgccg
     7861 gcgagcgcca tgtcgagcgc gactcctgcg ttggcgtcca cgcgcaggcc gaggtcgcct
     7921 tcgatcgcgc cgcgcgccac ttcgcgcaac cagtgaaaat cgaagccgaa acgccgcgga
     7981 agcgtgaatc gcatcgtcca ctcgccgtcg cgtacgatgg gcccgtccgc cagctcggag
     8041 tggcgcacaa cgagtttcgg atcggtcatt aagggtcctt tcagccgttt gattatacgg
     8101 atcggcttcg gaaaaacacg ttgtctgggc gtgcaaaggc gtcgccagcc tcgcgcaaac
     8161 tggcggctcg gaaccactta caggcggaaa gggattattt cgcaggcttg tgaacggccc
     8221 gctcagtcag tcgcattccc tcgaggtacg cgttcacgtc gccgagcgac gcgaacaggc
     8281 cgacatacga atcgctgtaa tacgacggcg agcgccgcgt gcgccggaag tcacgctcgc
     8341 gaaacaaatc cgagcgcgcc accacagccg cgcccatcgg ctgatactcg tggcgccctt
     8401 gcgcccacgc catctcgctg tcgacccaca acgcaaatcc catccttctc caattgttta
     8461 ctataagaac catgacccgc cgatcttttt tcccaaccgc cgccatgatc cccgccctcg
     8521 ccgccggcgc cgcgccgctc aagggccgcc tgaaacaatc cgcgacccgt agttgtttca
     8581 aggggatgcc gatcgaggac gccgcccggg aagctgcgca gcttggcctc aagggcctcg
     8641 acctcatcgg ttcgaatgac tggccggtgc tcaagaagta cgggctgatc tcgaccatgg
     8701 cgccgggcgg cggtacgatc gccgacggca tcaacaggcg cgaaaatcat gccaaaatcg
     8761 agcagcaatt gcacgaaaat gtacaaaaag cgcacgattt cggcctgcca aacgtcatta
     8821 cgctctcggg caaccgccgg ggcatggcag atgaggaggg catcgacaac agtgtcctgc
     8881 tgcttaacaa aatcaagggc agggccgagg accagggtgt caccgtctgc ctggaacttc
     8941 tgaacagcaa agtggaccac cccgactacc actgcgacca cacccgttgg ggggtcgagg
     9001 tctgcaagag ggtgaactct cccagggtca agctcttgta cgacatctat cacatgcaga
     9061 tcatggaggg cgatatcatc cggacgatcc gcgacaacat ccagtggatc gggcacttcc
     9121 acacggcggg caacccgggg cgccacgaga tcgacgcctc gcaggagctc aactaccgcg
     9181 ccatcgcgca ggccatcgtg gatctcggct acacaggcta cttcgcacac gagtactcgc
     9241 ccctgcggga cccgctgaaa tcgctggccg aggcaatcga aatctgcgac gtgtagccgc
     9301 tcagggcttt ccgaatttca ggcggacggt cgtgaaaccg tagggcttca ggggtaattc
     9361 gacgcggttg gcgctcacgg cggccggagt agcgccgtcc tcgacgccgt tcgtcaacgt
     9421 ggcagcgagg agcggaaaaa caggagacgt gacgtgcgcc gtccccggct ttccagcggt
     9481 ctcccgaagg cggaggatga aaccgtcgcc gtcctcagct tctttaaacg ccgtgacttg
     9541 cgcgtgggtc ggctcgatcg tcaggaacga gccggacgcg gcggccatca cacgcttcgc
     9601 cggcttcaga cggttgttgc cgagatcgta gaccgcataa gcgaagagcg gcgagcgtgt
     9661 ctcctggtcg aaacgggcaa gggcgtcgtc gcccaaatcc ctgccgctgg tgatcgcgta
     9721 gcggaacgtg aactcgccgc cctgcgccgc cttgtagttc gtgaaccagt agttgttcat
     9781 cgcgtacgaa agaacgtgtc cgttgttgac cgtgagatgc ttcagccaca gcccccggtt
     9841 gatgtccgcc agtgtgataa gcggcgcatc gggcgtggcc cacgcgatgg taatccccgc
     9901 gtcccctgcg cgtacgagat tctgcgtggt gaaccagtcg cggcacgcgc cgggtagctg
     9961 atcctcattc ggacgcaccc aagcgttctg cacctggtag gcaagctggg gcggcgagac
    10021 acggaagggg aaggcgaagt aaaccgcctc tttgtcgcgc gtttcgcgct tttccagacg
    10081 gttcacgatg tccacgcgct tgatgccgtc atagaggctg atctccgtgt cgatgactgg
    10141 aacgttctta gcctcggcgt gtacgcggat ccgttttcca tacggcgttc ggacggactc
    10201 gacgatgctc gcgttccgtg gcccgctcag atccaggcgt ggggccggaa gcgtcgcgag
    10261 gtcctgaagc atccggctcc gctcgccgcc ggtgacatag acaagctgat tcagcttgta
    10321 cggtgcagcg gtatcgacga ggtcgcgccc ggtctctttg tccagaagtc ctgcgacggc
    10381 gccggtggcc ggatcgaggg tcagccggta gaaggttgat tcgagttgcc actcctgagg
    10441 ctgggcctca gtgcccgacg gaaccttgcc tgcaatgttg cgcactgcgt aggcccggta
    10501 cccaagtccc ggaacgttct ccgcgaggaa acgcagcttg cgccagccgt ccgtctcgga
    10561 gacgacgtcc acggcgacgg gcttgccggt gaccatgtcg acgatctcgc gggaaggctc
    10621 gatctccgcg gttaccatgt cggagcgcgg ccacaggtcg gggttgaaca cgtagagcgt
    10681 gggcccgtcg acgctgatgt tctggacgag gcggttgaag gaccggtaaa gaagatcggt
    10741 cgtggcccag tgcgcgcgcc acgcgtaggc gcgcttgtgc tcccactggg cagtgacgaa
    10801 gcggcggccc ggctggttga cgctgctgtg agcgccccag gtgtgctcat cgtagaagag
    10861 gaggtttttc caggcgtcgc ggaactcggc ggcggggtag acctcatcag gccggaaggc
    10921 gctggctagg gcggcggcca tctcggcaac gggtagcata cgctgcgagt cgcggttcat
    10981 ggtggtttcg agggctgtgg aaccggcgcc gtcctcccaa taggcgccgg catcgccgcg
    11041 atagacgggg agtttcccgg cgaagttccg gctcaggtag tcgtagtagt cgccgtcggt
    11101 agcggcgatg atcttcgggt actcgaacgc ctcattccat tgcctgatgg tttcgacgtc
    11161 accgtggcct atgtcggcgt tgtctgtgta gagaccgtac agcagcacgg cgtccacggg
    11221 gtagttcgcg cggcggtaac gtgcaaggaa ctgggagacg gagcggcgca ggagatcgac
    11281 ggaaggattc tcgccggcga gccggtagaa ctggaggtaa gaccgcgcga accacgtcag
    11341 cacgcgg
//
LOCUS       DATIFQ010000533         5751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1909748, whole genome shotgun sequence.
ACCESSION   DATIFQ010000533 DATIFQ010000000
VERSION     DATIFQ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909748"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3125)
                     /locus_tag="VLE22_26685"
     CDS             complement(<1..3125)
                     /locus_tag="VLE22_26685"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB18067.1"
                     /translation="MPRLLLFGTIFGALSGLVFAQDYRARLQGTVTDSSQAIVVGATV
                     TLQNENTGVVAARPTDGNGQYIFDFVEPGSYSVTVTMHGFNKFVADKIPVQVRGDVTV
                     NATLTPGAVTETISVVDTAVALQFTTSTMELTIDRKMLTDLPIMQRNPFTLALLDPAV
                     VNRYWDVAHRNPFYMWSSSQLDVGGNTTMKNDITVDGAPVQIGVKGSYSPPMDAVQEF
                     SVQQNSVDAEFGHSGGGIMSVGMKSGTNEIHGTMYYFGRNPKFNARTNSVTNTRNLVR
                     NHIWGVTAGNPIIKNKLFTFTAYEGWRSQEPNTRISTQPTDLERQGDFSQSKSIYGGV
                     MTIYDPWTTTIDTATGKVSRTAFPDNRIPADRIDPTAKRIMQDIWAPNGPGDDVTGVN
                     NYKIGYSWPQKYFNFSERVDWNIQDNWKVFFRFSRVKTTLDQTDYVQSAAMPNDNGGA
                     MNNRNIAGDMVYTLNPTTVFNFRMSYGSLEDDYVAPRSMIGEEGLAEFWPNNPWYKPY
                     IGEMPAVYYPYISAGGNYGKGGYWIQHPRHWTWDGSLRKVLGKHSLKTGFAHRKHKAD
                     GIFPNLMNFYSYQHFTAETFQSPDLKKNGSGFATFLLGAIDNNSNAQTYPYQYFRANY
                     YGLFVHDDIRVNPRLTINLGLRYEYESGPYDERDRFSRYLDLTNPIPEFQNNAPKLPD
                     AVLQYRQSTPIWNGAWVFTDGSNRGIYEAQKLTLMPRAGIAIRVNDKTAFRAGFARYV
                     IPPVLIQNTLASNVLPMFGFSARTDVAPAINGIPQARLSNPFPSDYPLIMPLEKSLGR
                     YTNLGDPAWFYEQDMRTGVNDRVNFSIQRQLPYQIHFDGTWFMNFGHDLPYTKQLNLT
                     DPNLSYTHKAALNATVPNPFYQILTREKFPGSLRNLKTVTIGRLLSPYPQYGSIEQRF
                     SDGVLNRYKALQLRVQRSYSGGSSFLFAYNYNQEKNYEFFNSDDQFINKFTFIAGTMP
                     RHRVSAAGSYDLPFGKGRAHLNNLHPVLNAIVGGWQTSHLLMLSSGANLRFGGMLTDG
                     SSPKLEDRTPQ"
     gene            complement(3689..5221)
                     /locus_tag="VLE22_26690"
     CDS             complement(3689..5221)
                     /locus_tag="VLE22_26690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18068.1"
                     /translation="MSKYWFALLLWATVAWPAEKVFPHRWVYISRRLTSDQQVEEIRQ
                     IARTASENGLNGVLFSAGLDSIDLKPPDYLERLQKVREIFREYRLEMIPNIFSAGYGG
                     SILAHDRNLAEGLPVKDALYIAKGGEARIVPEVPVADSGAAVEGAWTCEVAVRPYRCY
                     RVTFRAKSEGLPETRPFTSGAFRLDVRTADNRNLTPWNARIPPTTDWREVRWGFNSLW
                     YDKVRISIGAASPRTGKAWVDSVRVEEVGLINVLRRPGTPVTVRSDGDGTIYKEGRDY
                     EWVVDPDLNFRFDHEGPALKLVVGGRIKDDERLRVSYYHGFSINDGQTTICMAEPKIH
                     EIWREMARQMHGAVAPPRYVLSMDEIREGGTCEACRGRNMAQLLGECLTKQFQMLREV
                     NPKVDVWVWSDMLDPNHNAHGNYYLVQGDFTGSWNYVPKEMGILAWYYQKRALSLPFF
                     SKLGFRTMAGAYYDADTLDNPRGWLEELQKTPNAQGIVYTTWQNKYALLEEFAKLVSG
                     KQ"
BASE COUNT         1054 a         1717 c         1708 g         1272 t
ORIGIN      
        1 ttctgcggag tcctgtcctc cagtttcggg cttgaaccgt ccgtgagcat ccccccgaac
       61 cgcaggtttg cgccggagct caacatcaga aggtggctgg tctgccatcc acccacgatc
      121 gcgttcagca ccgggtgcag gttgttcaga tgcgcccgtc ccttgccgaa cggaaggtca
      181 taggaacccg cggcgcttac gcgatgccgg ggcatcgttc cggcgatgaa ggtgaacttg
      241 ttgatgaact ggtcgtccga gttgaagaac tcgtagttct tctcttggtt gtagttgtac
      301 gcgaacagga agctgctgcc tccgctgtag gacctctgca cgcgcaactg tagggccttg
      361 taccggttca gcaccccatc gctgaatcgc tgctcgatcg agccgtactg cgggtagggg
      421 ctgagtagcc tgccgatggt caccgtcttc agattgcgca gcgagccggg gaacttctcc
      481 cgcgtcagga tctggtagaa cgggttcggc accgtggcgt tcaaggccgc tttatgcgtg
      541 tagctgaggt tcggatccgt aaggttgagc tgctttgtgt agggcaggtc gtggccgaag
      601 ttcatgaacc acgtcccgtc gaagtggatc tggtacggca actggcgttg gatcgagaag
      661 ttcacgcggt cgttcactcc tgtacgcatg tcctgttcgt agaaccaggc ggggtcgccg
      721 aggttcgtgt agcggcccag cgatttttcg agcggcatga tcagcggata gtccgacggg
      781 aacggattac tcaggcgagc ctgagggatg ccgttaatgg ccggggccac atcggtccgc
      841 gcgctgaagc cgaacatcgg gaggacgttg ctcgccagcg tgttctggat gagcacgggc
      901 ggaatgacgt agcgggcaaa gccggcgcgg aacgccgtct tgtcgttgac ccggatggca
      961 atacctgcgc gtggcatgag cgttaacttc tgggcttcgt agatgccgcg gttcgaaccg
     1021 tccgtgaaca cccaggcgcc gttccaaatc ggcgtgctct gccggtactg caacaccgca
     1081 tccggcagct tgggagcatt gttctggaac tcgggaatcg ggttggtgag gtccaggtac
     1141 ctggagaagc ggtcgcgctc gtcgtacggc ccgctttcgt attcgtaccg caagcccagg
     1201 ttgatggtga ggcgcgggtt aacccggatg tcgtcatgga cgaacagccc atagtaattc
     1261 gcccggaagt actggtaggg gtaggtctgg gcgttggagt tgttgtcgat ggcgcccagc
     1321 aggaacgtgg cgaacccact gccatttttc ttcaggtcag gcgactggaa ggtttccgcc
     1381 gtgaaatgct ggtaagagta gaaattcatc aggttcggaa agatcccgtc ggccttgtgt
     1441 ttccggtgcg cgaacccggt cttcaaactg tgtttgccca agactttgcg cagactgcca
     1501 tcccaggtcc agtgccgcgg gtgctggatc cagtagcctc ccttgccata attgccgccg
     1561 gccgagatat acgggtagta aaccgccggc atctcgccga tgtacggctt gtaccaggga
     1621 ttgttcggcc agaactccgc cagtccctcc tcgccgatca tcgaccgcgg ggcgacgtag
     1681 tcgtcctcga gcgagccgta actcatgcgg aaattgaaga ccgtggtcgg gttgagcgta
     1741 tagaccatgt ccccggctat gttgcgattg ttcatggctc cgccgttatc gttcggcatg
     1801 gcggccgact gcacgtagtc ggtttgatcc agagtcgttt tcacacgcga gaagcggaag
     1861 aataccttcc agttgtcctg gatgttccaa tccacgcgtt cggaaaaatt gaaatacttc
     1921 tggggccatg agtagccgat cttgtagtta ttcacgccgg tgacatcgtc gccggggccg
     1981 ttcggcgccc agatgtcctg catgatgcgc ttcgctgtgg gatcgatccg gtcggccggg
     2041 atccggttat ccgggaacgc cgtgcggctg acttttccgg tggccgtgtc gatcgttgtg
     2101 gtccacgggt cgtagatggt catcactccg ccgtaaatac tcttggactg cgaaaagtcg
     2161 ccctgccgct ctagatcggt cggctgagtc gaaatcctgg tgttcggttc ctggctgcgc
     2221 cagccctcat acgcggtgaa ggtgaacagc ttgttcttga tgatcgggtt gccggccgtt
     2281 acaccccaga tgtggttccg caccaggttg cgggtgttcg tgaccgagtt cgtgcgcgca
     2341 ttgaatttgg ggttgcgccc gaagtagtac atcgttccgt ggatttcgtt ggtgccggac
     2401 ttcatgccca cactcatgat tccgccgccg ctgtgcccga actccgcgtc cacgctgttc
     2461 tgctgcacgg agaactcctg caccgcgtcc ataggcggag agtaggagcc cttgacgccg
     2521 atctggaccg gagcgccatc caccgtgatg tcgttcttca tggtcgtatt gccgcctacg
     2581 tcgagttggc tggatgacca catatagaac gggttgcggt gggccacgtc ccaatagcgg
     2641 ttcaccaccg ccgggtcgag cagcgccagc gtaaatggat tgcgttgcat gatgggcagg
     2701 tcggtcagca tcttgcgatc gatcgtcagc tccatcgtgc tggtggtgaa ctgaagagcg
     2761 actgccgtgt ccaccacgct aatggtttcc gtgactgcgc caggcgtcag cgtggcgttg
     2821 actgtgacgt cgccgcgcac ctgcaccggg atcttgtccg ctacaaactt gttgaatccg
     2881 tgcatcgtaa ccgtgaccga atacgatccc ggctcgacga aatcaaatat gtactggccg
     2941 tttccgtccg tcggcctggc ggctacgact cccgtgttct cgttctgaag cgtaacggtg
     3001 gctccaacaa cgattgcctg gctggaatcc gttacggtac cttgcaagcg ggcgcggtag
     3061 tcctgggcga aaacaagccc ggagagcgcg ccgaaaatcg tgccaaacag cagaagtctt
     3121 ggcacgagac atctcgctga aagtctcatc ccataccctc ccactaaagt cggttggcta
     3181 gcgctgtgag gtaaactgac cgccgggttc aggcaagctg caacccgaca aaaccgccgg
     3241 aactggaatt tcaattcctc aaatccggtg tcgggctagt atacacccag gtatcaatat
     3301 ctggtcctct gaagtttgtt tttttctgcg gattgtcttt gccttttgcc aagagtcaac
     3361 tgcgccctgt agagaaaatc cacactccta gcctcttcta gcctgcctcc gcccatcccc
     3421 gccctgtacc cgacggccac cggaaacgca ccgacgaccg tgaatcgata cgatttccga
     3481 gataatcggc gtcgaacgac ggggctgagg tctcatcccc ccgttcgggg ggatgagagg
     3541 taacagtcgg tttaacgccg cgcggcctgc ggtaagatct ctcgtcgatc cgctaccacg
     3601 cctggctctc ccggtgaact gagggccgtt ctccggcttt gtgcgctagg aaagtgggag
     3661 aacgggtagt ccgtgctttg tcttcgattc actgcttccc actcacgagt ttcgcgaact
     3721 cctcgagcag agcgtactta ttctgccacg tcgtgtatac gatcccctgt gcgttgggcg
     3781 tcttctgaag ctcttccagc cagccacgcg ggttgtccaa cgtatcagcg tcatagtacg
     3841 cccccgccat tgtccggaaa ccaagctttg agaagaacgg caggctcagc gcgcgctttt
     3901 ggtagtacca tgcgaggatc cccatctcct tcgggacgta gttccacgat cccgtgaagt
     3961 caccctgcac cagatagtag ttgccgtgtg cattgtggtt cgggtccagc atgtccgacc
     4021 agacccacac gtccactttc gggttcactt cccgcagcat ctgaaactgt ttggtcaagc
     4081 actcgccgag cagttgcgcc atgttccgcc cgcggcacgc ctcgcacgta cctccttcgc
     4141 ggatctcatc catgctcagc acgtagcgcg gcggcgcaac cgcgccatgc atctgccgag
     4201 ccatctcgcg ccagatctcg tggatcttcg gttccgccat gcaaattgtc gtctgcccgt
     4261 cgttgatcga aaaaccgtga tagtaactga cacgcagccg ctcgtcgtcc ttgattcggc
     4321 ccccaaccac cagcttgagc gcggggcctt cgtggtcgaa tcggaagtta aggtcaggat
     4381 cgaccaccca ctcataatcg cggccctctt tgtaaatcgt cccgtcaccg tcgctccgga
     4441 ccgtcaccgg ggtgcccgga cgccgcagca cgttgatgag tcccacctcc tccacgcgca
     4501 cgctgtccac ccaggccttt cccgttcgcg gacttgccgc gccgatcgag atccgcacct
     4561 tgtcgtacca caggctgttg aacccccagc ggacctcgcg ccagtcggtt gtcggcggta
     4621 tccgagcgtt ccaaggtgtt aggttccggt tatccgccgt ccgcacgtcc aaccggaagg
     4681 cgccgctggt gaaagggcgc gtctccggca gaccttcact cttggcccgg aacgtcaccc
     4741 ggtagcagcg atacgggcgc accgcgactt cgcacgtcca tgcgccctct accgccgcac
     4801 cgctatccgc caccggcacc tcaggcacga tgcgcgcttc gccgcccttc gcgatgtaca
     4861 gcgcatcttt caccggcagt ccctcggcca agttgcggtc gtgcgccaga atactgcctc
     4921 cgtaaccggc agaaaagatg ttcgggatca tttccagccg gtactcgcgg aaaatctccc
     4981 ttactttctg gagacgctcg aggtagtcgg gcggcttgag gtcgatagaa tcgagtcccg
     5041 ccgagaacag gaccccgttg aggccgttct cggacgcggt gcgggcgatc tgccggatct
     5101 cctccacctg ctggtccgac gtcagccggc gtgaaatgta cacccagcgg tgaggaaata
     5161 ccttctcagc gggccaggct acggtggccc atagcaatag tgcgaaccag tatttgctca
     5221 tgccagccga attataacaa ccccgctccc tgcgctcact ctcgcgaccc atcagggtgt
     5281 gcaacgaaga gcagcgagag aggttttgtt gcttccgttt cgccaagggg gtaggagctt
     5341 acgcttggtt tccctacgtg gttgtaaagg aagggtttct ggagccaccc gccgggcttg
     5401 aaccggcgac ctgctgatta cgaagccagg gctgtcaagt ctggcgtgtg attttgtttc
     5461 tcgatttccc tttgattctg cggtggttgt ggatgcgttg ggcactcggg cgcacagccg
     5521 caaattggtg agatttcggg cgagttgctg acaaaactgc tgacaaaaac cggggctgac
     5581 tattgcgggc gatgctggcg aatggactcc agaatctggt cgagcgtcag gtccttttga
     5641 cactgctcgc gaacccgagt gtaatcgccg gttccagagc ggttcaggcg caggaaccgg
     5701 gccaggccat cggggccgag ctcgcgcatc aatacgccaa gagcatgccg c
//
LOCUS       DATIFQ010000534         8886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_378303, whole genome shotgun sequence.
ACCESSION   DATIFQ010000534 DATIFQ010000000
VERSION     DATIFQ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378303"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VLE22_26695"
     CDS             <1..319
                     /locus_tag="VLE22_26695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18069.1"
                     /translation="LPQYTDGNIFRARDGSVIVPFVSTWRLRRNAEGFDRDAKVICRL
                     PDAAEIRHVYAASVDLGTVVKVTPARDGDAIEITVPRHGKATMLVLVKQPDEALEAAV
                     QGR"
     gene            366..629
                     /locus_tag="VLE22_26700"
     CDS             366..629
                     /locus_tag="VLE22_26700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18070.1"
                     /translation="MRREADFLKDDEGVLVYIAKKLREAKALEELLTNSSVDYVVEVD
                     QYVGGFLFRTARAGAFFYVRADTETLTRELLARNGYRPYEPLE"
     gene            complement(634..3324)
                     /locus_tag="VLE22_26705"
     CDS             complement(634..3324)
                     /locus_tag="VLE22_26705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18071.1"
                     /translation="MRSLALFLLTVSLSLAADFRTGQAARLVIGQPTFTAQEPAGSQQ
                     VVGAASGLAYANDLLVVADSNRVGAGPINNRVLIYRNLSTMLPAPTASLDYTQRCPVC
                     VGSASVVLGQTDFTKIDVGLSETGMRLPTAVATDGTVLAVADTDNNRVLIWRSIPTTN
                     GAPADLVLGQPNLTSNAAPRPPTASSLRGPQGVWIQNGKLFVADTQNHRVLIWNAIPS
                     SNGQPADTVLGQPNFTTFVEVDLTKADLNVKPETLLNPVSVTSDGVRLYVTDLGHNRV
                     VIWNSIPASNQAPADIAIGQPDMTSAIFNNVRALCTPNGEDANGNDTYPPLCGATLSF
                     PRYALSDGQRLFIADGGNDRVLVYNSVPISSGQSADTVLGQFTAEFNSASDSADPLRR
                     SSSDSLRTPMSLAWDGTNLFVTDPFNRRIMVFTLAEPVIPYAGVRNAAGLKVYAVGGI
                     TFSGTLQENDEITITVKDKEYKYKLIKEDSFANIVNRLVEAINAGDGDPNVHATPNIV
                     FNAIILTARTEGSAGNGIIYSTKLSDNAKVTATTAGAQLAGGQDAAKIAPGTLVSILG
                     ERLAAEAVVAPGDADNLPLSLGGVEVYFDGIRAPLLYVSPTQVNAQIPWEVLDRTSLN
                     AYVRTVWPDGRVTATNPVAVPIIRQNPGIFTYGGDIDPRPAVMQHFSSNATGTVQIAG
                     TAKAGDVATVVIEDREYKYTVKTDDTTANIRDGLIAEINADPKVEAFAAGVYTWIRLR
                     ARIPGVEGNGIKFSAKTTDNSAVILTPTNEALCCANIAGSLVTDQNPALPGETIIVFA
                     TGLGLITPDDARQALHTGQKYSGPVLNEPEEFVSSLAGGKTANVLFAGVRPGMVGVYE
                     VHLELNTDIPTNPRTQLTIAQDLFVSNIITFPVFNPKPAQ"
     gene            complement(3559..4974)
                     /locus_tag="VLE22_26710"
     CDS             complement(3559..4974)
                     /locus_tag="VLE22_26710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB18072.1"
                     /translation="MAVDDSPGALKAEELAEVIRGIRERVLARYPRTAAPGDVALPDL
                     LPLVHARDAAEAKVAAIGTVNPRAPGVVNTLVQSAKRFVARLLDWHVREQVEFNRALI
                     YTIEALLETLNDYNRALVRLAESDAVTRARAEEAIQEARELKDIRSHWIEWRQEWERK
                     LSINEVQFLRGVADLQSAFQHRVTLMESNFRDLLRAQHSDFEGALNRSGTEIQRKLWA
                     DMEKIRTEYDRLIHNELRVVRQRVAATPPAAPASPIQAAPASPAFDYAPFAFRFRGTE
                     ESVRGKQRFYVPLFQDLGEVLDVGCGRGEFLELMREAGASARGIDSSEEQIAICREKG
                     LNAETADIFAYLAGLPEGSLDGIFCAQVVEHFRPDRIPEFVRLAASRLARNGLLAIET
                     PNPECLAIFATHFYLDPTHTRPVPSPLLRFYMEEFGLGDIEIRHLSPAADTMPSLETL
                     PAEFREAFFGGLDYAILGRKL"
     gene            complement(4958..5359)
                     /gene="queF"
                     /locus_tag="VLE22_26715"
     CDS             complement(4958..5359)
                     /gene="queF"
                     /locus_tag="VLE22_26715"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HSB18073.1"
                     /translation="MKKRYTDEHARAGLDAQLPEIETFPNQFPGYEIEIVIPEFTSVC
                     PKTGLPDFGKLTIRYVPDKLCLELKSLKMYELTYRNLGIFYENVVNRFLRDVVAACKP
                     VSATVIGEFTPRGGINSKVTASWSKSRGRRR"
     gene            complement(5356..6588)
                     /locus_tag="VLE22_26720"
     CDS             complement(5356..6588)
                     /locus_tag="VLE22_26720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HSB18074.1"
                     /translation="MSSGTVFPFQEVVEAQGHLIDSHVMEEIFDKVVECDGRFEVDQF
                     RIGRTNEDPSFLRLRIEAPTREAMDRLLAQLMDLGCSPAESGDVLLRVIERDRCAPED
                     FYSTTNHRTLVRLNQEWVPVEEQRMDALIVVKDGRAFCRRLRDIQAGDQAVCGMRGIR
                     VLPESKERDRLSFAFMTNGISSERQVETAVRQTAALIRHTVEQKQKVVVVAGPVTVHT
                     GGATPLAALIRAGYVHALLSGNALGVHDVEAALYGTSLGVRQSDGRQAEHGHRNHMRA
                     INAIYHAGGIRQAVESGKLTSGVLYECVKTGTPFVLAGSLRDDGPLPETITDMNEAQD
                     AYAQHIKGAGLVLCLGSMLHSIAVGNMSPSWVKIVCVDINPAVATKVSDRGTGQAIGV
                     ITDVGLFLELLARTLLLQ"
     gene            complement(6673..7029)
                     /locus_tag="VLE22_26725"
     CDS             complement(6673..7029)
                     /locus_tag="VLE22_26725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HSB18075.1"
                     /translation="MLRRGEIWLADLNPGKGTEPGETRPVLVIQAQALLDAGHPSTLV
                     IPLTTQLVDDAEPLRVRLSPSGKLHLESDLLIDQLRAIDNRRLVEGPLTRIGKPVLQR
                     VETAVLDVLDFSQPSV"
     gene            complement(7022..7258)
                     /locus_tag="VLE22_26730"
     CDS             complement(7022..7258)
                     /locus_tag="VLE22_26730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18076.1"
                     /translation="MKSISLKLSEDLLERSGKCAMELRMTRAAYVRTALDRMNHRVNK
                     ELRAKRLADVSRRVRAESLKVNAEFAAIEREPDA"
     gene            complement(7322..>8886)
                     /locus_tag="VLE22_26735"
     CDS             complement(7322..>8886)
                     /locus_tag="VLE22_26735"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HSB18077.1"
                     /translation="PVRCRVVEARSGQPLVARVRLLDSQGREVVPAGHPEKLGEDAHE
                     GDVRFQARRFAYVNGGFEVDPRMLPLKYQVIKGYEYGIAEGEITAEALKDGSFTIGLS
                     RWSELARRGWYSGDVHIHFIAPKTCRMEMDAEDLNVANILTGDFTPDVDQFEGKPNAN
                     SSGRHIVYVTQEFRNHELGHMCLLNLKKLVEPVKTVQPYHYPLHLDVCDKAHAQGGYV
                     TWAHFPSWPGVENPLDVAMEKLDGLEILSVLDPRDFPIFMKSVVPELESNHGLRLWYR
                     YLNCGFRLTPTAGTDKMTTFVTVGANRVFARIDGDFTYDNWIAALKAGRTFISNSPIL
                     SFSVNGREPGAALQLSSRKDKALRIHATAESQIPYTHLEIVSNGQVIGQARPGGSRNR
                     AEIHLEHPFSKSCWLAARVLEDIDPYRNRGIQFSKVHSDDGTLVSNYYGHRRPETVFA
                     HTSPVYVIRDSEPNRNWDDAQYYIRYLDNSIHWLKTEAKFARPADQEASIEAFQRGKA
                     IYEKRAAEARRG"
BASE COUNT         1637 a         2612 c         2899 g         1738 t
ORIGIN      
        1 actgccgcag tacacggacg gaaacatctt ccgggcacgg gacggcagcg taattgtgcc
       61 gtttgtctcc acgtggcgtc tgcggcgcaa cgcggaagga ttcgaccggg atgcaaaggt
      121 gatctgccga ctgccggatg cggcggagat ccggcatgtc tatgccgcct cggtcgatct
      181 cggcaccgtg gtcaaggtta cgccggcgcg agacggggac gccatcgaga tcacggtccc
      241 gcggcacggc aaggcgacga tgctcgtgct ggtgaagcag ccggacgagg ctctggaagc
      301 ggccgtccaa gggcggtgaa cctcagagta agaatccgag gggagctatt gtaaagtgaa
      361 aagcaatgcg acgggaagcc gactttctga aagacgacga gggagtgctc gtctacatcg
      421 ccaagaagct acgagaggcg aaggcgctcg aggagctgct gacgaactca tcagtcgatt
      481 acgtggtgga agtggaccag tacgtcggcg gtttcctctt ccggaccgcc cgggccggcg
      541 cattcttcta tgtccgggcg gatacggaga ctctcactcg ggaactgctg gcccgaaacg
      601 gctaccgtcc ttacgaaccg ctggagtgaa ccgttactgg gccggcttgg gattaaagac
      661 cgggaacgta atgatgttgc tgacgaagag gtcctgggcg atcgtcaact gggtccgcgg
      721 attcgtcggg atatcggtat tcaactccag gtgcacctca tacactccca ccatccccgg
      781 ccggactccg gcgaagagca cgttggcagt cttgccgccc gcgagggagg agacgaactc
      841 ctccggctcg ttcagaaccg ggccggagta cttctgaccc gtgtgcagcg cctgccgggc
      901 atcgtcgggc gttatcaggc cgaggccggt ggcgaagacg atgattgtct cgcccggtag
      961 cgcggggttc tggtcggtga caagactgcc ggcgatgttg gcgcagcaca gcgcttcgtt
     1021 cgtaggcgtc aggatgacgg ccgaattgtc ggtcgtcttg gccgagaact tgattccgtt
     1081 gccttccact ccggggatcc tggcgcgcag gcgaatccag gtgtacaccc cggcagcgaa
     1141 cgcttccacc tttgggtcgg cgttgatttc cgcaatgagc ccgtcacgga tgttggccgt
     1201 tgtgtcgtcc gtcttaaccg tgtacttgta ctcgcgatcc tcgatcacca cggtggcgac
     1261 gtcaccagcc ttcgcggtgc ccgcgatctg gacggttccc gtagcattgc tgctgaagtg
     1321 ctgcatgacg gcgggccgcg gatcgatgtc tccgccgtac gtgaagatgc cgggattctg
     1381 tctgataatc ggaactgcca cggggttggt agccgtgacg cgaccgtcag gccagacggt
     1441 gcgcacatat gcgttcaaac tcgttctgtc aaggacctcc cacggaatct gtgcgttgac
     1501 ttgggtgggt gacacataga ggaggggcgc gcggatgccg tcaaagtaaa cttccacgcc
     1561 gccgagactc agcggcaggt tgtccgcgtc acccggggcg acgaccgctt cggccgcgag
     1621 tcgttcgcca aggatgctga cgagcgtgcc gggggcgatc tttgcggcgt cctgcccccc
     1681 ggcaagctgc gccccggccg tagtagccgt aactttcgca ttgtccgaca acttagtcga
     1741 gtagatgatt ccgttgccgg ccgatccctc agtgcgagcg gtcagaatga tagcgttgaa
     1801 cacgatgttc ggcgttgcat ggacgttcgg atctccgtca ccggcgttga tcgcctcgac
     1861 aagccggttc acgatgttcg cgaaagagtc ctctttaatg agcttgtact tgtattcttt
     1921 gtctttgacg gtgatggtga tctcgtcatt ctcctggagg gtaccggaga acgtgatccc
     1981 accgacggcg tagaccttaa ggccggcagc gttacgaacg ccggcatacg gtattaccgg
     2041 ctcagcgaga gtaaagacca tgatgcgccg gttgaaagga tcggtgacga agaggttcgt
     2101 accgtcccaa gcgagcgaca tgggcgtgcg gagagaatcg gaactggacc ggcgcagggg
     2161 gtcggcgctg tcggaggcgg agttgaattc cgcggtgaac tggccgagta cggtgtccgc
     2221 actctggccg ctgctgatag gcacgctgtt gtagacgagg acgcgatcgt tgccgccgtc
     2281 ggcaataaac agacgctggc catcggagag cgcgtagcgg ggaaaactca gcgtcgcacc
     2341 gcacagggga ggataggtgt cattgccgtt tgcgtcctcg ccgtttgggg tgcagagggc
     2401 cctgacgtta ttgaagattg cgctggtcat gtccggctgg ccgatggcga tgtcggcggg
     2461 cgcctggttc gaagcgggga tcgaattcca gatcacgacc cggttgtgtc cgaggtcggt
     2521 gacgtagagc cggacaccgt ccgacgtcac ggagaccgga ttcagcaggg tctccggctt
     2581 gacgttcagg tccgccttgg tcagatcgac ctcgacgaac gtagtgaagt tgggctggcc
     2641 gagcacggtg tcggcgggct ggccgttcga cgacgggatt gcgttccaga tcaggacccg
     2701 gtggttctgc gtgtctgcga cgaacaactt gccgttctgt atccagactc cctggggccc
     2761 tcggagggaa ctcgcggttg ggggccgtgg cgcggcgttc gacgtaagat taggctggcc
     2821 aagcaccaaa tccgcaggag cgccgttggt ggttggaatc gatcgccaga tgagaacgcg
     2881 gttgttgtcc gtgtccgcga ccgcgagtac ggttccatcc gtggccaccg cggtgggcaa
     2941 tcgcataccg gtttcggaga ggccgacgtc aatcttggtg aaatccgtct gaccgagcac
     3001 aaccgacgcc gatccaacgc aaaccgggca ccgttgcgtg taatccagcg aagcggtcgg
     3061 ggccggcagc attgttgaca gattccggta gatgaggacc cggttgttga tggggccagc
     3121 gccgacgcgg ttggaatcgg cgacgaccag caggtcgttc gcataagcaa gaccgctggc
     3181 cgcgcccacg acctgctggc tgccggccgg ctcctgggcg gtaaaggtcg gctggccaat
     3241 gacaaggcgg gccgcctgtc cagttcgaaa atcggcggca agcgagaggc taaccgtcag
     3301 aagaaaaagc gcaagactcc tcatgataga aacgcggatg taccgagttt tcagtatagc
     3361 aggagcggga ccgcgccttt tctgcatggg caccgactac ccgaggacat ccgctccccc
     3421 ggggccggat gcaatctctc gggcgttgga cgaatggtgt gcttggttgg tttcttgaat
     3481 ccacgggccg gcccggaacc cagcgatgcc gtctctgttc cggggttttg ggaagctgtt
     3541 gaaaaaccta gcgtgtagct acagctttcg gccgaggatc gcatagtcca agccgccgaa
     3601 gaaggcctcc cggaattcgg cggggagggt ttccagggag ggcatcgtgt cggcggcggg
     3661 cgagaggtgg cgaatttcga tgtctccgag accgaactcc tccatataga aacgcagcaa
     3721 gggcgacggc acgggccgtg tgtgcgtggg gtcgagatag aagtgcgtcg cgaagatggc
     3781 aaggcactcc gggttgggag tctcgatcgc cagcagcccg ttccgggcga gccgcgacgc
     3841 cgccagccgc acgaactccg gaatgcgatc gggccggaaa tgctcgacaa cctgcgcaca
     3901 gaagattccg tcgagcgacc cctcgggcag cccggcgagg tacgcaaaga tgtcggcggt
     3961 ttcggcgttg agcccctttt cccggcagat cgcgatctgc tcctcgctcg agtcgattcc
     4021 gcgggcgctg gctcctgcct cccgcatcaa ctcgaggaac tccccccggc cgcaaccgac
     4081 gtccagcacc tcacccaaat cttgaaacag gggaacgtag aagcgctgct ttccgcggac
     4141 gctctcttcg gtgccccgaa agcggaaggc gaaaggagcg tagtcgaacg cgggcgatgc
     4201 cggcgcggct tgaatcgggc tcgcgggcgc ggcaggcggg gtcgcggcga cacgctgccg
     4261 cacaaccctc agctcgttgt ggatgagtcg atcgtattcg gtccggatct tctccatgtc
     4321 cgcccaaagt ttccgctgga tctccgtgcc ggaacgattc agggcgccct cgaagtcgga
     4381 gtgctgcgcc cgcaacaagt cgcggaagtt ggactccatc agcgtgacgc gatgctggaa
     4441 ggcggactgc agatccgcca cgccacgcag gaattgcacc tcattgatgg agagctttcg
     4501 ttcccactcc tggcgccact cgatccagtg agagcggata tccttcagct cccgcgcttc
     4561 ctgaatagcc tcctcggccc gcgcgcgggt taccgcatcg gattcggcaa gccggacgag
     4621 cgcccggttg tagtcattga gggtttcaag gagcgcttca atggtgtaga tcagggcgcg
     4681 gttgaattcg acctgttcgc ggacgtgcca gtcgagcagg cgggcgacga acctcttggc
     4741 tgactggacg agggtattca ccacgccggg cgctcgcgga ttgaccgttc cgatggcggc
     4801 gacttttgcc tcggccgcat cgcgagcgtg aacgaggggc aggagatcgg gcagcgccac
     4861 gtcgccgggt gccgcagtgc gcgggtagcg cgctagcacg cgctcccgaa tcccgcggat
     4921 tacctccgcg agttcttcgg ccttcaaggc tcccgggcta tcgtcgacgg ccacgggact
     4981 tgctccagct tgctgtcacc ttggaattga tgccgccacg cggggtgaac tcaccaatga
     5041 ccgtcgcgga gactggcttg caggcggcga cgacgtcccg caagaagcgg ttgacgacat
     5101 tctcgtagaa gatgccgagg ttgcggtacg tgagctcgta catcttgagc gacttcagct
     5161 ccaggcagag cttgtcgggg acgtagcgga tggtaagttt gccgaaatcc ggcaagccgg
     5221 ttttcgggca gaccgaagtg aactcgggaa tcacaatctc gatctcgtag ccggggaatt
     5281 ggttgggaaa cgtctcgatc tcgggcaatt gggcgtcaag cccggcccgt gcgtgttcgt
     5341 cggtgtagcg tttcttcatt ggagaagcag cgtccttgcc agcagctcga ggaagagacc
     5401 gacgtcggtg atgacgccga tggcctggcc ggtgccgcga tcgctgactt tggtggccac
     5461 ggctggattg atgtccacgc agacaatctt gacccagctc ggagacatgt tgccgacggc
     5521 gatcgagtgc agcatcgatc cgaggcaaag caccaggccg gcgcccttga tgtgttgggc
     5581 gtaggcgtcc tgggcctcgt tcatgtccgt gatggtctcg gggagcggcc cgtcatcgcg
     5641 gaggcttccg gcaaggacga acggtgtacc ggtcttgaca cactcgtaga gaacgccgct
     5701 ggtgagcttg cccgattcga ccgcctgccg gatgccgccc gcatggtaga tggcgttgat
     5761 ggcccgcatg tggttgcggt ggccgtgctc ggcctgccgg ccgtcgctct gccggactcc
     5821 gagcgaagtg ccgtagagag ccgcctcaac atcgtgaacg ccgagggcat tcccgctgag
     5881 cagagcatgc acgtagcccg cgcggatcag cgcagccagg ggcgtggcgc ctccggtgtg
     5941 gaccgtgacg gggccggcga ctacgaccac cttctgcttc tgctccacag tgtgacggat
     6001 gagggcggcg gtctgacgca ccgccgtctc cacctggcgc tcggaagaga tgccattggt
     6061 catgaaggcg aacgagagcc ggtcacgttc cttggattcc gggagtacac ggatgccgcg
     6121 cattccgcac acggcctggt cgccggcctg aatgtcgcgc agacgccgac agaacgcccg
     6181 gccgtccttg acgacgatca gagcgtccat gcgctgttct tcgaccggga cccattcctg
     6241 attgaggcga acgagcgtcc ggtggttggt agtggagtag aagtcctctg gggcgcaacg
     6301 gtcgcgctcg atgacgcgga gcaacacgtc gccggactca gcgggggagc accccaggtc
     6361 catcagttgg gcgagcaggc gatccatcgc ctcgcgcgtc ggcgcctcga ttctcaagcg
     6421 gaggaacgag gggtcttcgt tggtgcgccc gatacggaac tggtcgactt caaagcggcc
     6481 gtcgcactca acgaccttgt cgaagatctc ctccatcacg tgggagtcga tcaggtgccc
     6541 ctgggcttcc acgacctcct ggaagggaaa tacggtgccg gaactcattg ggatcgaata
     6601 ccagtctagc gaatctcact cagaggccgc gttagttttc tcctcgcagc ggagactgct
     6661 tttccaccag tgtcataccg acggctgcga gaaatccagc acatccagca cagctgtttc
     6721 cacgcgttgg agaacgggct tcccgatgcg ggtcagaggt ccctcgacga gacgccggtt
     6781 gtcgatcgcc ctgagttgat cgatcagcag gtccgattca agatgaagct tgcccgacgg
     6841 tgaaagccgc actcggagag gttctgcatc gtctaccaac tgggttgtga gcggaatgac
     6901 cagcgtcgat ggatgtccag cgtcaagcaa cgcctgagcc tggatcacta gaacgggccg
     6961 ggtctcgccg ggctccgtac ctttccctgg attcaggtcg gctagccaaa tctcgccgcg
     7021 cctaagcatc aggctcccgc tcgattgctg caaactcggc gttgaccttc aagctctcgg
     7081 cgcgaaccct tctggacaca tcggccaggc gcttcgcgcg gagttccttg ttgacacggt
     7141 ggttcatccg atcgagagcc gttcggacgt aagccgctct ggtcatgcgg agttccatgg
     7201 cgcacttccc gctcctctca agcaggtctt ccgatagttt cagggaaata ctcttcatgg
     7261 gatatattgc cagtatatca tcttgagata tctcggcctg ccacggcgcg gagagagcgc
     7321 gtcatcctct tcgcgcttct gccgcccgct tctcgtagat cgccttgcca cgctggaacg
     7381 cttcgatcga ggcttcctga tccgcgggac gcgcaaactt cgcctcagtt tttagccaat
     7441 ggatcgaatt gtcgaggtag cggatgtagt attgcgcgtc gtcccagttc cggttaggct
     7501 cactgtcgcg gattacgtag accggcgagg tgtgggcgaa gacggtctcg ggacgccggt
     7561 gaccgtagta attgctgacc agcgtgccgt cgtccgagtg caccttcgag aactgaatgc
     7621 cgcggttgcg gtagggatcg atgtcttcga gcacgcgggc tgcaagccag cagctcttcg
     7681 agaacggatg ctcgagatgg atctccgctc tgtttctcga tccgcccggc ctcgcctggc
     7741 cgattacctg tccgttcgag acaatttcca gatgcgtgta cgggatctgc gactccgccg
     7801 tggcgtgtat ccgcagcgcc ttgtcttttc gcgagctgag ttggagtgcc gcaccgggtt
     7861 cgcgtccgtt cacgctgaag gacagaatcg ggctgttgct gatgaaggtg cggccggcct
     7921 tcagcgccgc aatccagttg tcgtatgtga agtcgccgtc gattcgagcg aaaacgcgat
     7981 tcgcgccgac ggtaacgaac gttgtcattt tgtccgtacc ggctgtcggg gtcagccgga
     8041 atccgcaatt caaatagcga taccagagcc gtaacccgtg gttggattcg agctccggca
     8101 cgacgctctt catgaagatc gggaagtcgc gcgggtcgag aaccgatagg atctcgaggc
     8161 cgtccagctt ctccatggcc acgtcgagcg ggttctccac accgggccag ctcgggaaat
     8221 gggcccacgt gacatagccg ccctgcgcgt gagctttgtc gcagacgtcg aggtggagcg
     8281 gatagtgata cggctgcacc gtcttcacgg gctcgaccaa cttcttcagg ttgagcaagc
     8341 acatatgacc gagctcgtgg ttgcggaact cctgcgtcac atagacgatg tggcggccgc
     8401 tggaattggc gttgggcttg ccctcgaact gatccacatc gggggtgaag tccccggtga
     8461 gaatgttagc gacgttgaga tcctcggcgt ccatctccat gcggcaggtc ttcggcgcga
     8521 taaagtggat gtggacgtcg ccgctatacc agccgcggcg ggcgagttcg gaccagcgcg
     8581 acaatccgat ggtgaaactg ccatccttga gcgcctcagc ggtgatttct ccctccgcta
     8641 tcccgtattc gtagcccttg atgacctggt acttgagggg cagcatgcgg ggatcgactt
     8701 cgaacccgcc attcacatag gcaaagcgac gcgcttgaaa gcgaacgtcg ccttcatgcg
     8761 cgtcctcgcc aagtttctcg ggatggccgg ccggcacgac ttccctaccc tgcgaatcaa
     8821 gcaacctcac gcgcgcgact agcggctgtc ccgagcgagc ctccaccaca cggcatcgga
     8881 ccggac
//
LOCUS       DATIFQ010000535         7677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_760770, whole genome shotgun sequence.
ACCESSION   DATIFQ010000535 DATIFQ010000000
VERSION     DATIFQ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760770"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="uvrC"
                     /locus_tag="VLE22_26740"
     CDS             complement(<1..882)
                     /gene="uvrC"
                     /locus_tag="VLE22_26740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HSB18078.1"
                     /translation="MDLREKASQLPTAPGVYLYKDAHGTVLYVGKAKSLRSRVRSYFS
                     DDRLGDAKTGSLLGEARDIDYILVDNEKEALALENNLIKQWKPRFNVLLRDDKTYPYI
                     KLTGEKYPRVYVTRRLRKDGSTYFGPYFPGNLAHRLVHFIHRHFLVPSCKVDLTRRHA
                     QPCLQYHIHRCLGPCVEGLTTDEAYARAVQDVRLFLEGRHRDLAAGIRDRMEDASEEM
                     RFEEAAGLRDLLGTVEELQQRQKMASAEGDDADIFGYHAEPPLVAVNVFHLRNGRVVD
                     RREYFWEDQEEFEAGEFF"
     gene            902..1300
                     /locus_tag="VLE22_26745"
     CDS             902..1300
                     /locus_tag="VLE22_26745"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB18079.1"
                     /translation="MPPRRLLPAAVLALAGALFAQQEKQAPPPTEPPEEDEALVEKEY
                     AFNPIQAAKELKIGDFYFKKGSYPAAIGRYKEALKWNPGFADAWRRIGDAQEKRKDVA
                     AAREAWAKYLELEPEGKYASEIRKKLPGKP"
     gene            complement(1283..1843)
                     /locus_tag="VLE22_26750"
     CDS             complement(1283..1843)
                     /locus_tag="VLE22_26750"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HSB18080.1"
                     /translation="MEKTAEQVARIVRRCLDDGRPVEIDGLGTFRRRTGGEFEFVATA
                     APRVFLAYVQEDGAAAERLFQDFQNHGFDPWLDRKKLLPGQNWPRAIEHAIELSDHFL
                     ACFSHKSLQKRGQFQAELRYALDCAERMPLDRVYFIPVRIEDCKVPWMIRREIQYVDL
                     FPNWERGFRRILSAMRRQEKKLRLAG"
     gene            complement(1827..2051)
                     /locus_tag="VLE22_26755"
     CDS             complement(1827..2051)
                     /locus_tag="VLE22_26755"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSB18081.1"
                     /translation="MKKTDLAARLARQTRVSKAVAADELDRIVHDILRRLRRGKSAVL
                     PGLGEFKPGEMPAFEFEPQHRKDPSRGKDR"
     gene            2233..3630
                     /locus_tag="VLE22_26760"
     CDS             2233..3630
                     /locus_tag="VLE22_26760"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HSB18082.1"
                     /translation="MIARRQLLKSAAGAAAPLLARQPSRPHNVLLLIADDLGLHTVEY
                     GDRTAKTPNLDRLARDGVRFTNAYCTTASCSACRSVIMSGLHNHANGQYGHAHDFHNQ
                     SYLPFVRPLARVLKDAGHRVGLIGKLHVNPASAFPWDFSAPGNQRGVHEMAQAAKRFI
                     QASAGKPWYLHVGFGDPHRAAVGFGNQPHPGVTPNPFDPAKVSVPSFLPDNPETRREI
                     AEYYQAANRLDQGVGFMLDVLRETGELDNTLVMFFSDNGMAFPNAKTNTYDAGIHMPL
                     IVRSPAQQRRGLVNNAMISFVDLFPTVVEWCGARAPDYPVHGRSFLPILEQENPSGWD
                     RIYFSHTFHELTMYYPMRGMRTRQYKYIRNLYPELTYPHSTDLWASATWQSVLKQGRK
                     GKLGKREITRYLHRPGEELYDVVKDPDEVVDLAADPGHRSVLENMRAEVGEFRRRTND
                     PWLLNDAYLEGQRGL"
     gene            complement(3640..4305)
                     /locus_tag="VLE22_26765"
     CDS             complement(3640..4305)
                     /locus_tag="VLE22_26765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18083.1"
                     /translation="MGLALMMVLVMGGSYAVLLIYQAWKRNRYKQAVEVLKWLLAGIG
                     VYLVVLLAFSVPSGNRMMLAGEEQKFCGFDMECSLGGSVIRVQRRKTIGNPPRELVAE
                     GMFYAVTVRVTTAKPSPKFKPRDLTGYVLDVAGNRYDRFTQAEWELVSPDAKDPYEIT
                     SGPSGGSFKKTMVFDVPVSAQEPSLVLQEGSWIERTLQLFLVADEGSLLHGRILHRLI
                     VRE"
     gene            complement(4322..5995)
                     /locus_tag="VLE22_26770"
     CDS             complement(4322..5995)
                     /locus_tag="VLE22_26770"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HSB18084.1"
                     /translation="MKQVLLLGFAALLSGQDFDVVIRGGQIVDGTGNPWYFGDVAVKG
                     GRIVAVGRIEGRTAARTIDAGGLTVAPGFIDIHNHSDETILVDGNAESMVRQGVTTMI
                     FGEGGSAAPVGGKQGPPDPDVTWRDLDGYFARVLKQGVSTNIGTYVGSSQIWTYVHGP
                     RAGPITKEEAEGMRALVREAMQQGALGVASSLSGPPGSWIDTAALVAMCEEAARFGGI
                     YSTHMRTEGQGVFESVAEALEIGRRAGLPVDVIHLKIAEHKLWGQMPELAGSLAQARA
                     NGQEVTANVYPYRAGQNNLSSIIPPWAHEGGAEALIARLKDPRLRPRLEQEILNGIPG
                     SNWYNHYTATGGWEGMLLVSLSNPSYKWFEGKRMNEVIQALGKEPVDVLFELLVNNGG
                     SVPTVYFHHSEEDMRYALRQPFVSIGSDGTALKTEGPLSRGNPHPRYYGTFARVLGRY
                     VREEKVLTLEEAVRKMTSANAAKIRQFDRGLLRPGQWADVTVFNAATVIDHATFEKPH
                     QYSSGIEYVLVNGEVVLERGRHTGARPGKILYGQGRTSGKPAEASKGGR"
     gene            complement(6023..7234)
                     /locus_tag="VLE22_26775"
     CDS             complement(6023..7234)
                     /locus_tag="VLE22_26775"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB18085.1"
                     /translation="MKLKRRDVLAAMAAPLAGAAAGCTARKPAEKAVETAKKGKPLDL
                     SEFQPKSMLVVPETKVEKAKFPVVDVHTHFSFPGKAEAGVTWGETLVYLAPPEELLAV
                     MDRRNIQALVNLTGGYGNGLKECIEKYDKKHPGRFVTFTEPMWEKTNQPDYAKAQAAA
                     IEEAGKAGAKGLKITKVLGLYLREGITSGALVKIDDPRFDPMWEACAAAKFPVAIHVS
                     DPVAFFEPIDRFNERFEELNAHPDWSFHGKDFPSNAEILEARNRVFARHPKTQFIALH
                     AGNFGENLGNVSECLDKFPNMHLEIGARIGELGRQPRTARKFFDKYQDRIMFGTDAVP
                     HGDDTPQQVYGDLLYQIYYRFLETEDEYFDYAPAPVPPQGRWRIYGVGLPDKILGKVY
                     NENAKRLIRLT"
     gene            complement(7248..>7677)
                     /locus_tag="VLE22_26780"
     CDS             complement(7248..>7677)
                     /locus_tag="VLE22_26780"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB18086.1"
                     /translation="RVLARHPKTQFLGLHVSNWPEDLGLVSQWMDKYPNLWVEIGARI
                     AELGRQPRMARKFFEKYQDRILFGTDIFTRGKKAADMASLGRVYPIYYRFLETEDEYF
                     NYSPGAVGGSGRWRIYGLGLSDKILRKVYNENAKRLLSLA"
BASE COUNT         1399 a         2568 c         2178 g         1532 t
ORIGIN      
        1 gaagaattca ccggcctcga attcctcctg gtcctcccag aaatactcgc gccggtccac
       61 gacgcgtccg ttcctcaggt ggaagacgtt caccgctacc agcggcggtt cggcatgata
      121 gccgaagatg tccgcgtcgt cgccttcggc cgacgccatc ttctgcctct gctgcaactc
      181 ctccaccgtc ccgagcaggt cgcgcagtcc ggctgcctcc tcgaatcgca tttcctcgga
      241 cgcatcctcc attcgatccc ggatgccagc cgccaggtcc ctgtggcgcc cctccaggaa
      301 cagtctcacg tcctggacag ctcgcgcata cgcttcatcc gtcgtcaggc cctcgacgca
      361 cgggccgagg caccggtgga tgtggtactg gagacacggc tgcgcgtggc ggcgcgtgag
      421 atcgaccttg caggacggta ccaggaaatg gcggtggatg aaatgaacca ggcggtgtgc
      481 gaggtttccg gggaagtacg gaccgaaata ggtagagccg tccttgcgca accgccgtgt
      541 cacgtacaca cgggggtatt tctcgccggt cagtttgatg taagggtacg tcttgtcgtc
      601 gcgcaacagg acgttgaagc gcggcttcca ctgcttgatc aggttatttt cgagcgccag
      661 cgcctctttt tcgttatcga ccaggatgta gtcgatgtca cgcgcctccc cgagcagact
      721 cccggtttta gcgtccccaa gccggtcgtc ggagaagtag ctcctgaccc gcgaccgcaa
      781 gctcttcgcc ttgccgacgt aaagcaccgt tccatgcgca tccttgtaca ggtacacccc
      841 gggcgccgtg ggtagttgcg aagctttttc tcgaagatcc atcggcggct atactttaat
      901 tgtgccacca cgccgcctcc tgcccgccgc tgtcctggcg ctcgcagggg cgcttttcgc
      961 ccagcaggag aagcaggcgc cgcctcccac ggaaccgcct gaggaagacg aagccctcgt
     1021 cgagaaagaa tacgcgttca accccatcca ggccgccaag gagctgaaga tcggcgactt
     1081 ctactttaag aaggggagtt acccggctgc gatcggacgc tacaaagagg ccctaaagtg
     1141 gaacccggga ttcgcggatg cgtggcggcg gattggagac gcccaagaga agcgcaagga
     1201 cgtcgctgcg gctcgggaag cctgggccaa gtatctggaa ctcgaacccg aagggaagta
     1261 cgcctccgaa atccgcaaga aactacccgg caagccgtag tttcttctcc tgcctccgca
     1321 tcgccgacag aatccgccgg aacccgcgtt cccaattggg aaacaagtcc acatattgga
     1381 tttcccgcct gatcatccag ggcactttgc agtcctcgat ccgcacggga atgaagtaga
     1441 cgcggtccaa cggcatgcgc tccgcgcagt cgagcgcgta acgcagctcc gcctgaaatt
     1501 ggccgcgttt ttgtagcgat ttgtgcgaaa aacacgccaa aaagtggtcg gaaagctcga
     1561 ttgcgtgctc aatggcgcgc ggccagttct gtcccggcag gagtttcttc cggtcgagcc
     1621 acgggtcgaa gccgtggttt tggaaatcct ggaatagccg ctccgccgcc gccccatcct
     1681 cctgcacata cgccagaaaa acccttggcg cggcggttgc gacgaactcg aactcgcctc
     1741 cggtccgccg ccggaacgtc cccagcccat cgatctccac cggccggcca tcgtcaaggc
     1801 agcgacggac aatccgtgct acctgctcag cggtcttttc cacggcttgg atccttccga
     1861 tgttggggtt cgaactcgaa cgccggcatc tcaccgggtt tgaattcgcc caaacccggc
     1921 agcacggcgg acttgccccg acgcaaccgg cgcaaaatat cgtggacgat tctgtccagt
     1981 tcgtcggcgg caacggcctt cgacacccgc gtttgtctag ccagccgcgc ggccaagtcg
     2041 gtcttcttca tgccgactcc atgctagcag cggactccgc ggagactccg cgaaacggcg
     2101 agtcagccgc aggtcctcac atccgtgcaa atctttgatt cgttgatgga tatctcactc
     2161 agcccgaatt tcttcccaga tctgtgactg aactcccttg ccgggcgtgc tctactgtaa
     2221 ggggaattcg tcatgatcgc tcgccgacag ctcctgaaat ccgccgcggg tgcagccgct
     2281 ccgctgcttg cgcggcagcc atcccgtccg cacaacgtcc ttctcctcat tgcagacgac
     2341 ctcggccttc acaccgtgga atacggagac cgaacggcaa agaccccgaa cctggatcgt
     2401 ctcgcccgcg acggagtccg ctttacgaac gcctactgca ccactgcctc gtgcagcgcc
     2461 tgccgctccg tgatcatgag cggtctgcac aaccacgcca acgggcagta cggccacgcc
     2521 catgacttcc acaatcagtc ttacttgccc ttcgtgcgcc cgctcgcccg cgtgctcaag
     2581 gacgccgggc accgcgtcgg gttgatcggc aaactgcacg tgaacccggc gtcagccttt
     2641 ccgtgggact tcagcgctcc gggcaaccag cgcggcgttc acgaaatggc gcaggcggcc
     2701 aagcggttca tccaggcctc ggccggtaag ccgtggtatc ttcatgtcgg tttcggcgat
     2761 ccgcaccgtg ctgccgttgg ttttggcaac caaccccatc ctggcgtcac gccgaatccc
     2821 ttcgaccccg cgaaggtttc cgtgccgtct tttcttcccg acaaccccga gacaaggcgg
     2881 gagatcgccg agtactacca ggcagccaac cggctcgacc aaggcgtcgg cttcatgctc
     2941 gacgtcctcc gagagaccgg cgaactcgac aacacgctcg tcatgttctt cagcgacaat
     3001 ggcatggcgt ttcccaacgc gaagacgaat acctacgacg ccggcataca catgcctctg
     3061 atcgtacgat cgcccgccca gcagcggcgc ggcctggtca acaacgcgat gatcagcttt
     3121 gtggacctct tccccaccgt agtcgagtgg tgcggtgcaa gggcgccgga ctatcccgtc
     3181 cacgggcgca gcttcctgcc cattctcgag caggagaatc cgtcaggttg ggaccgcatc
     3241 tacttctccc acaccttcca cgagttgacc atgtactacc cgatgcgcgg aatgaggacc
     3301 aggcagtaca agtacatccg caatctctac ccggagctga cctaccccca ttccaccgac
     3361 ctgtgggcgt cggcgacgtg gcagagcgtt ctgaaacagg ggaggaaggg caaactcggc
     3421 aagagggaga tcacgcggta tctacaccgg ccaggcgagg agctttacga cgtcgtcaag
     3481 gatcccgacg aggtggtaga cctggccgcc gatcccggac accggagcgt gctcgaaaac
     3541 atgcgcgccg aggtcggcga attccgccgc cgcaccaacg acccctggct tctcaacgac
     3601 gcctatctcg agggccagcg cggcctttga tccgccttac tattctctga cgatcagccg
     3661 gtgcaagatc ctcccgtgga ggagactgcc ctcgtcggca acgagaaaaa gttggagcgt
     3721 tcgctcgatc caggaaccct cctgcaacac caacgacggc tcctgggcgc tcaccggaac
     3781 gtcgaatacc attgtcttct tgaaggagcc gcctgacggg ccgctggtga tctcgtatgg
     3841 gtctttcgcg tccggactca caagctccca ttccgcctga gtgaagcggt cataccggtt
     3901 gcccgcgaca tccaacacgt aaccggtcag gtcccgcggc ttaaacttgg ggctcggttt
     3961 cgcggtcgtc acccggaccg tcactgcata gaacatcccc tccgccacca gttcccttgg
     4021 ggggttgccg atcgtttttc tccgctggac ccggataacc gaacctccga gcgaacactc
     4081 catgtcgaag ccgcagaact tctgctcttc accggccaac atcatccggt tcccgctggg
     4141 tacggaaaaa gccagtagta cgacgagata gaccccgatc cccgcaagca accacttcaa
     4201 aacttcgacg gcttgcttat accggttgcg cttccaggcc tggtaaatca gaagtactgc
     4261 gtagctgccg cccatcacca gcaccatcat caatgccagt cccatcgcga atctcctgag
     4321 tttacctgcc gcccttgctt gcttccgcgg gctttccgct cgtccggcct tgaccgtaaa
     4381 gaatcttgcc cggccgggcg cctgtgtgcc gtccccgctc gagcacgacc tcgccgttca
     4441 cgagcacgta ctcgattccg ctggagtact ggtgcggttt ttcgaaagtg gcgtggtcga
     4501 tcactgtggc cgcattgaac acagtaacat ccgcccactg tcccggccgg agcagcccgc
     4561 ggtcgaactg gcgtatcttc gccgcgttcg ctgacgtcat cttccgcacc gcctcctcga
     4621 gcgtgagaac tttttcctcc cgcacgtacc ttccgagcac gcgagcaaac gtgccatagt
     4681 atcgcggatg cgggttgccg cgggacagag gcccttcggt cttcagcgcg gttccatccg
     4741 atccaattga gacgaacggc tgccgcaaag cgtaacgcat gtcctcttcg gagtggtgga
     4801 agtaaaccgt cggcacagac ccaccattgt tcaccagtaa ctcgaacagt acgtcgaccg
     4861 gctccttgcc cagtgcctgg atcacctcgt tcatccgctt tccttcgaac cacttgtaac
     4921 tcgggttcga aagggaaacg agaagcatcc cctcccagcc gccggtcgct gtgtagtggt
     4981 tgtaccagtt gctgccgggg atgccgttta ggatctcctg ttcgaggcgc ggccgcaggc
     5041 gcggatcttt gagccgcgcg atcaaggcct ctgcaccgcc ttcgtgagcc cacggcggaa
     5101 tgatacttga gaggttgttc tgcccagccc ggtacgggta gacgttggcc gttacttcct
     5161 gcccgttcgc cctggcctgt gccagcgagc cggccagttc gggcatctgg ccccagagtt
     5221 tgtgttccgc gatcttcagg tggatcacat ccaccggcag gcccgcccga cggccgatct
     5281 ccagcgcttc ggccaccgat tcgaagactc cctgaccttc cgtgcgcatg tgggtcgagt
     5341 agattccgcc gaagcgcgcc gcctcttcgc acatggcaac cagggccgcg gtgtcgatcc
     5401 acgagcctgg tggaccgctc agcgagctcg ccacaccgag ggcgccctgc tgcatcgctt
     5461 cacggaccag cgcgcgcatc ccttccgctt cttccttggt gataggcccg gcccgcggcc
     5521 catgcacgta cgtccaaatc tggctcgacc cgacataagt tccgatgttc gttgagacgc
     5581 cttgcttgag aacgcgtgca aaatacccgt cgaggtctcg ccatgtgacg tccgggtccg
     5641 gagggccctg cttgccgccg acgggcgcgg cggagccgcc ctcgccgaaa atcatcgtgg
     5701 tcacaccttg ccgcaccatg ctctcggcgt tgccgtcgac cagaatggtt tcgtccgagt
     5761 ggttgtggat gtcgatgaac ccgggggcta cggtaaggcc tccggcgtcg atcgtccgcg
     5821 ccgcggtgcg gccctcgatc cgccccaccg cgacgattcg gccgcctttg acggcgacgt
     5881 cgccgaagta ccagggattt ccggtgccat cgacgatctg cccaccccgg atgaccacgt
     5941 cgaaatcctg gccggaaagc aacgcggcga acccaagcag aaggacctgt ttcatggcga
     6001 tgcgcttcgc gacgcgccct atctacgtta accggatcag ccgcttcgcg ttctcgttat
     6061 atactttgcc caggatcttg tcaggcagcc ccacgccgta gatccgccag cggccttgcg
     6121 gcggcaccgg cgccggagcg tagtcgaagt attcgtcctc ggtctcgagg aagcggtagt
     6181 aaatctggta gagcagatcg ccgtacacct gctgcggtgt gtcatcgccg tgcggcaccg
     6241 catcggtacc gaacatgatc cggtcctgat atttgtcgaa gaacttgcgc gccgtgcgtg
     6301 gctggcgccc gagctcccca atccgcgctc cgatttcgag gtgcatgttc gggaacttgt
     6361 ccagacactc gctgacattg ccgaggtttt cgccaaagtt gccggcgtgc agcgcgatga
     6421 actgtgtctt cgggtgccgc gcgaacaccc ggttgcgcgc ttcgaggatc tcggcgttgc
     6481 tcggaaagtc cttaccatgg aacgaccagt ccggatgcgc gttcagctcc tcgaaccgct
     6541 cgttaaaccg gtcgatcggc tcgaagaacg ccaccgggtc ggagacgtgg atggctaccg
     6601 ggaatttcgc cgccgcacag gcctcccaca tcgggtcgaa gcgcggatcg tcgatcttca
     6661 cgagcgcgcc gctagtgatg ccctcccgca ggtacagccc cagcactttg gtgatcttca
     6721 ggcctttggc gccggccttg ccggcctcct ctatcgccgc tgcctgggcc ttggcgtagt
     6781 ccggctggtt cgtcttctcc cacattggct cggtgaaagt gacgaaacgc ccggggtgct
     6841 tcttgtcgta cttctcgatg cactccttca gcccgttgcc ataaccgccg gtcaggttca
     6901 ccagcgcctg gatgttccgg cgatccatca ccgccagtag ttcctccggc ggcgccaggt
     6961 agaccagcgt ctcgccccac gtcacgccgg cttctgcctt accggggaaa ctgaagtgcg
     7021 tatggacatc gaccaccgga aatttcgcct tctcgacctt ggtttccggc acaaccagca
     7081 tgctctttgg ctggaactcg ctcaggtcga gcggtttccc tttctttgcc gtctccactg
     7141 ctttctcggc cggtttccgc gccgtgcaac cggccgcggc gcccgccagc ggtgccgcca
     7201 tcgcggcaag tacgtctctg cgtttaagtt tcatggttaa tcccatacta cgccagcgaa
     7261 agaagccgct tcgcgttctc gttgtagacc ttgcgcagaa tcttgtcgga cagaccgagg
     7321 ccgtagatgc gccagcggcc cgaccctccc acggctcccg gtgagtaatt gaagtactcg
     7381 tcttcggttt cgaggaagcg gtagtaaatc ggatagaccc gcccgagcga cgccatgtcc
     7441 gccgcctttt tgccgcgggt gaagatgtca gtgccaaata ggatccggtc ctggtatttc
     7501 tcgaagaact tccttgccat tcgcggctgg cggcccagtt ccgcgatgcg cgctccgatc
     7561 tcgacccaaa ggttcgggta cttgtccatc cactggctca ctaggccgag gtcttcgggc
     7621 cagttggaaa catgcagccc gaggaactgt gtcttcgggt gccgtgcgag cacgcgg
//
LOCUS       DATIFQ010000536        48062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2483775, whole genome shotgun sequence.
ACCESSION   DATIFQ010000536 DATIFQ010000000
VERSION     DATIFQ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 48062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48062
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483775"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VLE22_26785"
     CDS             <1..510
                     /locus_tag="VLE22_26785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSB18087.1"
                     /translation="LAGTKERLTAAGVRITREMPEQSQFFAEFPDGVLVEFTGDPKLD
                     APIKHHHLHLASNQIDEMRAWYAKVFGAVPGMRGKFKAADLPGVNLSWKTAEKPPVPT
                     KGRRLDHIGFEVTDIKAFCAKLEAGGIKLDMPVTPRPDLGLVIAFITDPWGTRIELTE
                     GLSRLSAGN"
     gene            complement(549..1205)
                     /locus_tag="VLE22_26790"
     CDS             complement(549..1205)
                     /locus_tag="VLE22_26790"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HSB18088.1"
                     /translation="MNRMFLAVLPFLGPAGAAYFLWSHYDQLPARFPVHFNAVAQADR
                     WVGKSPGAVFMVPAIAAGSLALMLAILLMIQYSSRPLRGETAVRRHRLNVNMLLVVMW
                     MVSFLTTVAALLPVIPQPIAARLIWASVAGLLLTVAGFAIPLMRMSTEPSEEGDGTPA
                     GCWKLGVFYYNPADPSLMVEKRCGLGYTLNFGNRRSWGLMALMLVIVLAPLFFILRMK
                     "
     gene            1256..2299
                     /locus_tag="VLE22_26795"
     CDS             1256..2299
                     /locus_tag="VLE22_26795"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HSB18089.1"
                     /translation="MPSRYLSAWQAHSRRGFLSALGGAAMLRPMACAPQFATRGVVLI
                     PEDLTLPDWPDRAKQSGLTTVALHHGRSVFEVIKSVTSGAGQEFLARSRRLRLEVEYE
                     LHAMSELLPRWMFEEDKSLFRMNEKGERVPDWNLCVHSTRALEVASENAVAVARLLRP
                     TTSRYFYWGDDGRPGCRCGKCRELSDGEQALLLENHMARALRRHDSRAQLAHLAYSTT
                     LAAPRQVKPEPNVFLEYAPIARRYDIPYSEQMEGRDGLANLDANLRVFPAATAQVLEY
                     WLDVSRFSRWKRPAAKLPWNRDVFLADVRTYAARGIRHITSFACYIDAEYVRRHGEPW
                     AISEYGAGLRQRM"
     gene            complement(2334..3791)
                     /locus_tag="VLE22_26800"
     CDS             complement(2334..3791)
                     /locus_tag="VLE22_26800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB18090.1"
                     /translation="MIAVLVVAAALGGGAEDIPTLFREARQAEQRRDFAGALEKYSAV
                     LRMDATIAEVWANQGLVLYELGRHREALHAFEKAVELKPQLTSPQLFLGLEYLRFEEP
                     RKALAPLGAALKLDPANAQTGYALADAQAKLGHFEEAIALYRGVLSRQPGMEEARYAL
                     AIAYLNFSKSIARKLIDSGSGYGKLLLAEYLAIGDANEAAEANYQAALEALPESGEVR
                     GALRAFYLRRNQPEKAEAISDMEPDSAPDPLAPALALWRDGSYEEVFQSLLPSDTQRE
                     RYWLSLTCYALAREILVESVRRNPNSLHACLLLADLAKNAGDSAAAQAAYEKAARLAP
                     DNVEAQLLFIRFIAPRDPAAALEYTQQAADRSDNEELNVELGMQLLKSDNAGAAAGRF
                     RRALEREPDLAKAHAGLADAYAKSGEFAPAIAEMKHAIAADPDGSWHYRLATWYKEVG
                     RLAEAREAFAATAQIKAQQRAKEEARFLELTSPDR"
     gene            complement(3788..4813)
                     /locus_tag="VLE22_26805"
     CDS             complement(3788..4813)
                     /locus_tag="VLE22_26805"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB18091.1"
                     /translation="MAARAVALALCAMFLAAIALSQDVPVPQRIREMLNARNYSGAER
                     AIAEELRRLPEWSTGHLLIAQIYARTGRYELAERSASAAIRQRESLDGLMLLAVATMR
                     LGRLNDSISWLEQAARRHPDHAEIYKILGLDYALGGVLRESEKAFAKAVALQPGDWEG
                     HYYQGRTLFELRRFREAHDALQRAVDLNRASVKGWTALGQTQERLGKVEAAETSYKQA
                     LSACENGRECAWPLLQLGFLYRTRNAPEQALALFRRAVEARPDWARPHFHLGKELAEI
                     GDLRAARKELEEAVKLDESRSEYHYQLGLVYRWLGEAEKASAQLARFRALADLKSHVE
                     VPDFAQP"
     gene            complement(4872..8375)
                     /locus_tag="VLE22_26810"
     CDS             complement(4872..8375)
                     /locus_tag="VLE22_26810"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB18092.1"
                     /translation="MQSKWVPIVCIAALMLALGVCSYGQSDRGSLTGLVTDPTSAPVP
                     RVTVTVTNAATNTAREALTSETGVYVFQELPAGTYNMSVKHTGFRAYVQNGITVGATQ
                     TVTQNVSLQVGQLTETIEVHADASMLKTESAEVSTSVTQEKLSALPIDFGGAMRNPMS
                     FLRLVPGSSVSRDQSWPVTSQNGLQSFSEEIRIDGASSTNPTPGVFNEAQPSVDAIQE
                     ISVQTANFNAEYGQAGGAIMNFTLKSGTNNLHGTAYEYLRNEFLNARNKDSGEKTRLR
                     RHEFGGTIGGPVVFPKLYNGKNRTFWFTSYEEYYDREKQQGFYDVPRSEWRNGDLSSL
                     LTGNVLGNDVLGRPIQQGQIYDPATTRTEVVNGQSYVVRDPFANNQVPLRSEVAKKIL
                     SFLPPAGIAGLDSNNLLGIAGTPLRDEIIWSLKIDHDFSPKSRLSGSFNYMYTHKING
                     ATPFGAADPARDQTITSKIARLNHDYTFGSTALNHFTVGLLRYQNPDGVPDRGFDPEA
                     QLGLKGTLLKGWFPAVNIDGLSAFGTQQLKHLYHTVPTVVDSLSKTVGSHTLKFGVEY
                     RKSNANFFGGNGAYGGLSFNAAQTALPYLSGSSGNYSLLGSAFASFLLGQAGPGSYLN
                     SAGHYSYRYSDYAFYAQDDFKITPRLTVNYGLRYDLHRPLTEKYGRIASFVPTLPNTG
                     AGGRPGALGFLGSGSEPGRTGRDSWLDTDKRDFGPRIGAAYRVSDKTVVRGGFGVVYG
                     RLEVNTFDPIQSNGVGSVNAQYPAIDPATQAQFSLDSGFPPVTVVPPVFDVTLLNNQG
                     LAAYRPESGRLPRIYNWNFTVQRQITPNLTVEAAYVGNHGTRLITGNFVNLNQNDFGV
                     LSLGDKLLRQINNAADAQALGVTYPYEGFSGTVAQALRPFPQYRGIGDPQATVGESDY
                     NSLQVKATQRLSKGVDFLVAYTLSKAITTVDDAFGWGGAGSVDAKKLSLERALATASN
                     NPGDRTHNLAVAFGYELPLGNLVTNAAAKHVIGGWKIAGILNYASGSALGMGYPNNLG
                     NVIFNNAGRYDIVSGQSLENKVDSVWPGQAWMFNINAFKAPDAYTVGNAARAYSAIRG
                     FPYVNEDLALSKQIHFTEGTTLELRMDALNAFNRSIWNNPDTGVTNTQRIQGGRAIGF
                     GSFWGRSNLERQMQAQIRFVF"
     gene            complement(8882..9166)
                     /locus_tag="VLE22_26815"
     CDS             complement(8882..9166)
                     /locus_tag="VLE22_26815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18093.1"
                     /translation="MWRNSNGNDAERKTNCIDNNAARAWLEQNIPFFKSSDKELESSG
                     IGAGVHAFADGEEIGSSPRLKWFRAKRPERDGRNTTAIHFRFRVLTLQRT"
     gene            complement(9339..10253)
                     /locus_tag="VLE22_26820"
     CDS             complement(9339..10253)
                     /locus_tag="VLE22_26820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB18094.1"
                     /translation="MRLQSLILKLPPALQLAAEHWPIRVFRWKHDLAVRAALRSGPPY
                     RPSHKRIIIDITASCDLGCMDCNRSCGAGQAPAPEHMTVDQVNRFISESKLQHRYWNA
                     IQIEGGEPTRHPQFLEIVNTLHSYVRRDSPSTVIQVNTAGYSQFSRGMVRQLPPAVIV
                     YCSEKTQVVQPGHLTFNVAPIDLPGHSKYDFSQGCFLPSMYGLGLTRYGYYPHPICGG
                     IDRVFGMDIGRKTLPDAADLLDELFPRLCRYCGLFVHYNRALQPALPKRSTDSAGDSK
                     KFPSGTMTESWSKAYAGYRAQPPSLRHY"
     gene            complement(10250..11497)
                     /locus_tag="VLE22_26825"
     CDS             complement(10250..11497)
                     /locus_tag="VLE22_26825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB18095.1"
                     /translation="MVTSFFGAQSFGGDSVYVERLCRALLRRGHEVHVVRSPGAFESV
                     RGSTPLRSYEPPDGLIEHRLGTGWRGKLDALWSHQTAGAGRNAATLRWIFEHEAFDVI
                     HLHNISLLGGLGVPRVASKLSRAVKLLTAHDYWWICPQSLLWKFGRSVCEARACFTCN
                     LRRGVPPQLWRRAGWVNDAFSGLDAILFPSTSTMRIYEAAGVKNHRLRVLPGLLPANW
                     ESRGQAPAGLAPGLLPARPFIAAAGRLVVEKGFQTVIPLMRQVPGLDLVIAGDGPLRT
                     ELELIASGMPNVKFVGLLPNDEVRLLFQHARAVVVPSLFPETFGLVLAEALALGAPVI
                     ARCVGAIPEVAATSSRVMMFDGAKGLLNILREISQSSGELGACADGGTARTPAVWSED
                     GHLVEYFGLISEIRNQGQTTVRQ"
     gene            complement(11506..12423)
                     /locus_tag="VLE22_26830"
     CDS             complement(11506..12423)
                     /locus_tag="VLE22_26830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSB18096.1"
                     /translation="MNRKSAPRASVVIPAYHSSSTIADCLDGLRGQTFRDFELIVVNS
                     SQESETERIVRALYPEARFVQSPTRLLPHAARNLALQQARGELLVFTDPDCVASRDWL
                     SCLATASDSGDGAIVGAMGLGRRAWWEAGVHLCKFHGLLPGLRPGVKRCAPTANAAYS
                     RELWKDIGPFPGHLFAGDGIVSWRAAKCGQPPRFLPDAIVLHRHLEGFSGFCRQRFQR
                     GRDFAVAQTELMDPPGVITWLRLLFSWAAIGWVLGRAALDALRAGWGLAFFLTLPIQV
                     VGHELWAVGETWGAARLLTRRLVESGSIR"
     gene            complement(12420..13364)
                     /locus_tag="VLE22_26835"
     CDS             complement(12420..13364)
                     /locus_tag="VLE22_26835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSB18097.1"
                     /translation="MRPPSLSVVAITGQDRRRADGLLAALAGQTITSDIEAILIDVGS
                     ETLPDIIPPTGLRASILRRPGSDDWGAARADGVRQAQAPVVAFLEDHTLPTARWAEEV
                     DRAFVRKDRDVAAACYAFTNGSPDTYFYRSVFMAEYGALAHPLPDGPAPTATANNIAY
                     RRDVLLALGERLESLLEMDFFLHRFMGRDFRVLSVPRALLAHQTNTTLRDLMVGHFEY
                     ARFFANRRLRHETWNLPKRIVAAMAVPLLVPALRLKRLFSALRGRPLLRDALAGLPVI
                     LLLFVCGGFGEAWGLLRSGELSASRLIWLELSAPRAGR"
     gene            complement(13511..14884)
                     /locus_tag="VLE22_26840"
     CDS             complement(13511..14884)
                     /locus_tag="VLE22_26840"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSB18098.1"
                     /translation="MTHTVSFIAVIAFAASAVVSPSPAASGPVGAPEGWKTAAPRDEI
                     KPLFSYEPKAGRSAHGAFVIAADGREGLDGYWFRSFSVEGGKYYSFLAYRKTTGVAVP
                     RQSALVRIKWLDDRGEEVKDDRKLVERYLNNFNATAPPEFPTDKQTDAAGWTEVSDTH
                     QAPGGARQAVVELHLQWAPKGRILWSDVSFTAVSPPSPRKVRLASIHFRPREGKSPER
                     NRRLFAPLIEEAARRGADLVVLPETLTFYGTGKTFAEVAEPVPGPSTEYFGELAKKHN
                     LYIVAGLVERNGHLVHNVAALLAPDGSLAGKYRKITLPDGEVEAGIAPGHEYPVFQTR
                     FGKLGIMICYDGFFPEVARELTKRGAEVIAWPVWGCNPELARARAAENHTYLVSSTYE
                     DISSNWMLTAVWDHAGRTIASAKDFGTVILAEVDLNEHTRWRSLGDFRSKIPRHAPVV
                     PPEQAGK"
     gene            complement(14966..15517)
                     /locus_tag="VLE22_26845"
     CDS             complement(14966..15517)
                     /locus_tag="VLE22_26845"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HSB18099.1"
                     /translation="MARNSIGLIELGSIAAGYEVTDAMLKTADVDLVLARSICSGKYM
                     VMVRGDVAAVQASVDAGSRAGSFSVIDTFVIPNVHEDVFPAISGSSKVETLEALGILE
                     SFSVASLIEGADAAAKSANVKLIEVRLAMALGGKAFVTLTGNVAAVRSAVESGAQVIA
                     KKGLLVNKVVIPQPRKELLSEMI"
     gene            complement(15524..16885)
                     /locus_tag="VLE22_26850"
     CDS             complement(15524..16885)
                     /locus_tag="VLE22_26850"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSB18100.1"
                     /translation="MSKFRPPRNGGVLANSMGLDKKLRECGVVGAGGAGFPTYIKANS
                     EVEFMIANGAECEPLIHKDVELMTHFPAEILAGMDLMTKATGASRGKFGIKEKNAAAV
                     EAVRNHLKPEKTDLVFLGDFYPSGDEYELVYTATGRLIPPAGIPLQVGCVVNNVETLY
                     NVHFANKGVPVTKKFVSIAGAVREPKSFWAPIGTSFRELIELAGGARIAEFGMFVNGM
                     MMGFLSFDLEDVVTRTTAGLIVLPRDHYLITRKDRPIESMNHIGKSACDQCSYCTEFC
                     PRYLLGYEVQPHKVMRSLGFTSTGADIWNQWAQLCCACGLCTLYACPEDLFPKEACDQ
                     AKVDMRAAGIKFVQEKEVSVHPMKEYRRVPLAQLRKRLKVEEYEAETPYEALDYQPKS
                     VRIKLSQHAGKPAKPAVEESDAVDAGAVIGKVEAPDMGVNIHSSIGGKVTRVTETFVE
                     IRA"
     gene            complement(16860..17228)
                     /locus_tag="VLE22_26855"
     CDS             complement(16860..17228)
                     /locus_tag="VLE22_26855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18101.1"
                     /translation="MIRNFRHFQAGPDPFGRTWQVDFLWLQTAISIRHSDSVDVKFLL
                     SSGDMKVEKVIALRHPDLLELARQTGRPVTDPWCARLAALHLVRMITTGEDLEKTLIT
                     VMKHDLAQYNTMLEQIPAAS"
     gene            complement(17264..17878)
                     /gene="folE"
                     /locus_tag="VLE22_26860"
     CDS             complement(17264..17878)
                     /gene="folE"
                     /locus_tag="VLE22_26860"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HSB18102.1"
                     /translation="MKKRKAVVEVMQPRHHSGEIAANVERILLELGEDPSRDGLLKTP
                     HRAEAALRFLTSGYHMNVEKLLNGALYAVKYDEMVVVKDIEFFSLCEHHLLPFFGKIH
                     VAYLPKNKVIGLSKIPRIVDMYARRLQLQERLTQQVAQTIMETIEPLGVGVIAEAKHF
                     CMMMRGVEKQHSGAVTSAMLGAFRHRKETRDEFLSLVSHSGMQF"
     gene            complement(17875..18303)
                     /locus_tag="VLE22_26865"
     CDS             complement(17875..18303)
                     /locus_tag="VLE22_26865"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSB18103.1"
                     /translation="MIRLTRRYRFSSAHRLHAAQLSDNENRDVYGKCNNPFGHGHNYT
                     VEVSVRGPLDQATGLVVDLQALDALVRERVVDAFDHKDLNRQAAEFAATPPTTENLAI
                     VIRDRLLAGWDDTFPGGGTGLERIRIYETKRNIFEVNGKS"
     gene            complement(18300..18716)
                     /locus_tag="VLE22_26870"
     CDS             complement(18300..18716)
                     /locus_tag="VLE22_26870"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSB18104.1"
                     /translation="MFITRKVEFSASHVCRNASLTDEENKSLYGAAANPHGHGHNYVL
                     EVTLEGSPDPVTGMVFDLKRLKEVLNREVVEPFDHRFLNREVPPFDRVVPTTENVAVE
                     IWNRIEPHFRGPAARLYSIRVYETEDLYVDYFGGTH"
     gene            18778..19299
                     /locus_tag="VLE22_26875"
     CDS             18778..19299
                     /locus_tag="VLE22_26875"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSB18105.1"
                     /translation="MQTVKTERVLRILIGILLVVLGYVIVDSFEERIVVVGDTAPDFT
                     VTTDSGRQVTRSDFGGKLLVLNFWATWCPPCVEEMPSLDQFQKRLAGDGVVVLGVSVD
                     KSEQAYRQFLRKAKVSFLTARNPSAEISAEYGTFKYPETYLIDGRGKVVLKHIGPRDW
                     SDERLINEIRSLL"
     gene            19306..20736
                     /locus_tag="VLE22_26880"
     CDS             19306..20736
                     /locus_tag="VLE22_26880"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSB18106.1"
                     /translation="MISEQAKRELAEVVGPRGFLDRPEDLTLYEYDASVEKARPDLVV
                     FPRSTDEVVRIVKIASRHGVPLVGRGAGTGLSGGAIPVNGGVIVSFARMDRILEIDLE
                     NERAVVEAGVVNLDITIAVQGQKYFFAPDPSSQKACTIGGNVAENAGGPHTLAYGVTT
                     NHVLGLEAVLSDGTVITTGGKELDLPGYDLTGLLTGSEGTMALITKVVVRLMRQPEMV
                     KTVLAIYDTTEGAGNTVSEITARAITPVAVEMLDGVMLRMVEEATHAGYPMDASAVLL
                     VELEGLREAVEEQVEQVREACEACGAREFRVARSAEERDLLWKGRKNAFGAVGRVSPF
                     YYVQDGVVPRTQIAPTLRFIGQVSEKYGLTISNIFHAGDGNMHPIILFDARKPGDLEK
                     ARAAGEEILLHCISVGGTITGEHGVGMEKNELMPKLFSEDTLETIGRFKQLFDPQCFF
                     NPGKLLPTGKGCIEIRQPAVTSETML"
     gene            20733..21683
                     /locus_tag="VLE22_26885"
     CDS             20733..21683
                     /locus_tag="VLE22_26885"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB18107.1"
                     /translation="MTTRRQFLFLPAALAAAAPKLGHKERVDRALRGEDVDRPPFSFW
                     YHFGLQKFPAERHANATLRFHRKFRTDIVKVMSDFPYPKPKGNWYDPVVVDDPFPEQI
                     RALEMIREGLGGKAHFVETIFNPWNVAEKLSSKEEVQKLKSEKPQVLLDVLEAIAKSE
                     ANHARRAITTGASGIFLAIANAGDGVLDESGYAKFSEPFDRMVLEAASGAPLNTLHLH
                     GAKIYLDRFWKGWQAPVVNYSAHGTGVGIAAARSKYDGVIMGGIDEQNYRTLTPAQLK
                     EQWRVAQQAAGKKFFLAPGCSVPNETTDRELMNLVRVLGA"
     gene            21706..22593
                     /locus_tag="VLE22_26890"
     CDS             21706..22593
                     /locus_tag="VLE22_26890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HSB18108.1"
                     /translation="MRQQGAFSRTLLLSLCLALSAAGVAAGAEYPPAASYTCYRAEGP
                     IRVDGRLNESSWVKAPRSNPFVDIVTGRPAWHDTRVALIWDDRYLYFGYTIEEPYVQA
                     TLTERDSKIYIENDVEMFIAGKDAYYELELNARNTVYEVFWIWKDIYKPGSPYFGVPE
                     FDVKSHTMMTLSGVGGHIHPRGERWGFLDWDLPRLKTAVFVDGTLNNPKDRDRGWTVE
                     LAVPWQGLKWLADGRALPPRAGDVWRVDCSRFQKFGPDGKPMDGSAGWTWNRHGHYDS
                     HIPETFTRVRFSAEKVGRE"
     gene            22649..23083
                     /locus_tag="VLE22_26895"
     CDS             22649..23083
                     /locus_tag="VLE22_26895"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSB18109.1"
                     /translation="MTERHTEEEAFVSLQKTADALMQGIAVLLKPAGLSATQYNVLRI
                     LRGAGKDGLACGEIGVRMITRDPDITRLLDRLETRGLVTRARELKDRRVITARITKDG
                     LRMLKELDAPIQRYHESQLGHMGEKKLVELIWLLELARRPSI"
     gene            23202..23900
                     /locus_tag="VLE22_26900"
     CDS             23202..23900
                     /locus_tag="VLE22_26900"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSB18110.1"
                     /translation="MITLRHRDERGVSRLGWLDSRHTFSFDHYYDPRHMGFRSLRVIN
                     DDRIAPGAGFPTHGHRDMEIVSYVLAGALDHKDSMGTGSTIAAGDVQRMSAGTGVMHS
                     EFNHSQSEPGHFLQIWILPERKGLVPGYEQRTFSTQEKRGRLRLVASRDGRDGSVTIH
                     QDADLYATVLEPGEGATHALRQGRAAWVQVARGKVVVNGTALEAGDGASVTGESTLEI
                     EGKEAAEVLLFDLG"
     gene            23932..24738
                     /locus_tag="VLE22_26905"
     CDS             23932..24738
                     /locus_tag="VLE22_26905"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSB18111.1"
                     /translation="MNETIDFLPRHGYAVLFVFVLAEQVGLPVPALPVLLAVGALAAS
                     GQFGFAPALAVAVVAATAADLVWYELGRRRGHTVVNLMCRVSLEPDTCVRRTEDTFAR
                     YGLRTLLFAKFIPGLNTVTPPLAGMVHTPMGRFVLHDAAGAAIWAGAFMGLGYLFSTE
                     LERVAVHAMQLGGGLFVLLAGGLACYIVWKYVQRKRLFWRLRTSRIAPEELKRQMDKG
                     NPPIIVDLRHSVDLDVDGFRIPGALHMKPAELERRHGEIPRGREIVLYCT"
     gene            24876..25847
                     /locus_tag="VLE22_26910"
     CDS             24876..25847
                     /locus_tag="VLE22_26910"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HSB18112.1"
                     /translation="MRRTAKIVVLLAFAVVVSGAQAGEARSRTDWFHDARWGVFIHYL
                     AKSDDVSVDEWNRVIDGFDAEGLAQQLESARAGYLILTLGQNSGHYLSPNATYDALTG
                     IRPSKCSRRDLVDDLYRVLAPRGIRLMVYLPGGAPDRDKVAMEKLEWKTGPHRNLEFQ
                     RKWERVIAEWSERWGRKVSGWWFDGCYWPNAMYRSMNPPNFASFAGAARKGNADSIVA
                     FNRGVIFPIISMTEQEDYTAGEMDDPSKVQVSEFWLDGAQFHMLSYLGKAWSAGPPRF
                     TTDQVVEFTSRITRKGGVVSWDVPTDARGLILEPFLEQLRAIGAVVR"
     gene            complement(25854..27062)
                     /locus_tag="VLE22_26915"
     CDS             complement(25854..27062)
                     /locus_tag="VLE22_26915"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSB18113.1"
                     /translation="MKRLLLVLLVPVLAVVWWVYRRSSAAPEVPFTKVKRETLVSTLV
                     TNGKVEPLEWVEIRAEHGGIVDRVLVEVGQPVARGAVLAQLDANTARTELTAAEARVM
                     QARAELEVLQRGGRSTELAEIESALRRARSDSEIAGRDVESLRRLVEKQAATRQDLDQ
                     ATQRLERAQADIRSLERKRAALVSDTDRTAAQARLWEAEASVELARSRIASASIRSPI
                     GGVVYEKDVRLGSYLSPGDPVAKVGQIDKVRVRVYVDEPELGRVAQGLPVTITWDALP
                     GRKWTGAVERMPTGIVAQGTRQVGEVICIIENPNRDLLPGTNINAEIRSRVVENAVTV
                     PREALRRRSQQAGVFVLQGDKIAWRNVTLGPSSITRAQVLDGLGEGDAVALPSDTPLQ
                     EGLAVQPVYR"
     gene            27159..27920
                     /locus_tag="VLE22_26920"
     CDS             27159..27920
                     /locus_tag="VLE22_26920"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSB18114.1"
                     /translation="MGANSKTVVVFSAGMMFGAYQAGAWSALAKELRVDGVVGASSGA
                     LNAWVVAGGCDPDTLREMWLSNEIASVTRLRAPLPPWSGIFDSKRLERLVRGIYDSFR
                     PRMEIGIVATEVRNFRRRLFREEEITWRHLAASCAIPFGFQPVRIDDHFYTDGGMLGA
                     LPLWAASEMGATRIVAVLAMPRLPFALGRAVLKVVRAAAPETSRVPLGLQIEVIQPSE
                     PMGSLRDLILWRRENAERWMDLGFRDGAEALRRLV"
     gene            27961..29478
                     /locus_tag="VLE22_26925"
     CDS             27961..29478
                     /locus_tag="VLE22_26925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18115.1"
                     /translation="MNPNNGGCCASTCTTCPVSRRGFLTAGMAASVLPAVSASSMKDP
                     ARQQPIRMALKVQPVLVYDLYKRREATSWRPWGGLMNEQDVAAEKERIGKELSSMKTS
                     ADFPLEVLPLATARNQDEAAAIARGDSDVIISYAASGDGRIFETLTVPGKWHLMFLRH
                     RSGPVYLWYEIAHPRFLRKTVDEFGQPGMNVHDVVVDSHAEILIRLRALGALKNTMGK
                     RIVAVGGPSGWGAGGRNAPDLAREQFKFDLQTVPYPELGERIKRARADSALVKRCQGE
                     AEMYLRDKAVRLETSREFVQNSFVLTEVLRDLLDEAKTDALTINQCMGTIMPMSETTA
                     CLPLSLLNDDGYMAFCESDFVVIPSGVLLHYISGKPVFLNDPTHPHDGVVTIAHCTAP
                     RKMDGMHAEPVRVLTHFESDYGASPKVEMRKGTKMTNLVPDFASRKWIGFEGEVIDNP
                     FMAICRSQVDITIEGSTEKLLEQMGGFHWMSSYGAYVKETKYALRKIGVELVQLT"
     gene            29541..29885
                     /locus_tag="VLE22_26930"
     CDS             29541..29885
                     /locus_tag="VLE22_26930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18116.1"
                     /translation="MPVYRIHRLKENQRQQFRWAPHTTGATDVKPKDYEQAGSVEATN
                     SYSAWFALKDGEDALKVGDLLESESGDLLIYKYVGFEAARWVLPEVKPGIESTPPAVE
                     NSRNEGAAAFSG"
     gene            29895..30467
                     /locus_tag="VLE22_26935"
     CDS             29895..30467
                     /locus_tag="VLE22_26935"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HSB18117.1"
                     /translation="MQSDTDPGGRINLFSLVAYIPDPLGGYMDQLRQELVPSCRLRAH
                     VTVLPPRPISEEAGKAIEQLRSQLRDLVPFDVQAKDVDMFSITSVVYVAVGAGFNKLR
                     RMHDVLNSGALKFCEPYPYHPHLTLAQQMTPEQIGGVYETARIRWAQCPFERVFPVDV
                     LTFVQGTTMGTWIDLAECPIGQPVATPGRS"
     gene            complement(30477..30959)
                     /locus_tag="VLE22_26940"
     CDS             complement(30477..30959)
                     /locus_tag="VLE22_26940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18118.1"
                     /translation="MAASTSKKVVIRRFDREPVTGFVNPHDYLQPEGIELLSPTGSVL
                     LVPYGEIKAVCFVRDFEAPGGSASPGLFHTRPKTEGLWVRMCFNDGELMDGLLPNNLL
                     QLEAFGFTVVPPNPVSNNQRIFVPKAALREFHVVGVVGSPIQRRRKKGAPTDQMEMFE
                     "
     gene            31022..31921
                     /gene="xerD"
                     /locus_tag="VLE22_26945"
     CDS             31022..31921
                     /gene="xerD"
                     /locus_tag="VLE22_26945"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HSB18119.1"
                     /translation="MRETSLIGTQVRAFLNSCRVEKGLAENTVEAYRRDLGKFLSFIG
                     VEAAGEIPRADELGRYLDSLYRSGLASRSIARHLTTLRNFYGFLAREGQIESDPTSFL
                     TSPRQWKNLPKYLNLEEINRLTMAPDPARPTGVRDRAMLELLYATGLRVSELCNLELS
                     DLDPALGILRVTGKGGKQRIVPVGKAAVAAIEAYVASARPQLLRSRGSRYLFVTARGG
                     RLTRQAFWKLLAVHGKRVGIFRRLTPHVVRHSFATHLLEGGADLRSVQMMLGHADIST
                     TQIYTHVLRSRLRKTVDQHHPRR"
     gene            31966..33342
                     /locus_tag="VLE22_26950"
     CDS             31966..33342
                     /locus_tag="VLE22_26950"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18120.1"
                     /translation="MSDYMFMLENHLSAPQNRVVKEVQAAAEHGNVNLFLTGGAVRDM
                     LGGFPIRDLDFTVEGNALKVAKLAAERAGATLLKADEERRTADLIFPGSVTVEIGMAR
                     SERYSKPGKRSHVSQSTIVEDLRGRDFTIDSIALSLNRASLGLLLDPNNGQADLEHRE
                     LRAVHNHIFFEDPGRLLRLVRFRVRFGMVVDDRTQQQYENARLEALERLIPPRGLFKE
                     LREIADETSPGEVLAALETEKLLPLFSEALAGPKLNTAGLAKWQKAKQAIPFGVEIGA
                     NNLALLLYLLTEKLTPKERAALVKSVSMHKSEIDLWQTLEAKTRKLERSVGSAKIHKP
                     SHIYRILSSAAGEQILFLLIRSQIRLVHDRIKNYLQKYLPAAQEITDAQVAAAGLQPG
                     TTKFRKQKEEMIVARLDGRVRKPAAAEEPEEHAAAVPASAERTPYGTNRSAKISPGRK
                     PGSAGARR"
     gene            33275..34570
                     /locus_tag="VLE22_26955"
     CDS             33275..34570
                     /locus_tag="VLE22_26955"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB18121.1"
                     /translation="MGPIDRRRFLLGASLALPVRGAETKIQIGTVGVRGRGRDHIEEI
                     ARRPDSLVAAVCDVDTAQTERAVALVEKLQGSKPKVYQDYRKMLDDQDVQAVSIATCN
                     HWHALHAIWACQAGKDVYVEKPASHNIWEGRRMVEAARKYSRVMQVGMQSRSIQHKRR
                     AMDLLRQGVIGKIYMAKGICYKRRPSIGKKPDGPVPPGVNYDLWLGPAPMRPFNENRF
                     HYNWHWFWDTGNGDIGNQGVHEMDIARWGLNKSTLPKVVHSSGGKFVYDDDQETPNTQ
                     LSVFEWDDCQLHFEVRGLLTGGESSIAYDGRNFIGNMFFGSDGYMSVDQRGFQVYRGD
                     GRELTERMDYAEPRIWDTAPHIGNFIEAVKSRNATDLHADILEGHLSASLCHMANISY
                     RTGRKLTFDPVSETFVNDPEANGLVKRKYREPYVVPAQV"
     gene            complement(34580..34933)
                     /locus_tag="VLE22_26960"
     CDS             complement(34580..34933)
                     /locus_tag="VLE22_26960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSB18122.1"
                     /translation="MKTPVSSMLLVLLASFIGSFGAVFLKSGAGRLHRQIRTLILNWR
                     LALGVAMFLLSSFFFVLGIRRGELTILYPMVSLGYIWTLFWSRLFFGEPFTRNKFVGI
                     GLILLGIAFLNLGNR"
     gene            complement(34930..35331)
                     /locus_tag="VLE22_26965"
     CDS             complement(34930..35331)
                     /locus_tag="VLE22_26965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18123.1"
                     /translation="MNTASTHPPAKKSFQFNRKQSIVLVFFCTLFAAAAQILIKSGAN
                     TLQSSSPLAMITNLPLMAGYSLYGIVTLLLVLALRDGELSILYPVISLTYVWVAVLSV
                     VFFHEAVNPFRVVGVLIIVTGVAVLGKNGKQ"
     gene            complement(35420..36334)
                     /locus_tag="VLE22_26970"
     CDS             complement(35420..36334)
                     /locus_tag="VLE22_26970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18124.1"
                     /translation="MRIPLILFSAFLLAAAACGQEQLPAPGSPNGVLQNTGAPISVPL
                     ECGDEEIQSAGLACTADSPCTVYLELAAVEPVGARLFVTGNLHTASSTLSSVLLASDD
                     SGKTWSEPHERIRLAILEQIQFLDFETGWVGGQLVRPLPRDPFLLLTSDGGKTWRQQP
                     LFEESRIGAIEKFWFDSRTEGSLWLDRTQSGEIDSRHERYETMTGGESWSIREAGAKP
                     LPVKGVAAAPRDTGWRVRAHAASGSYRIEKRQGKSWQTVAAFSIELGQCKPKEQILPE
                     PMVESPKPSEGNPAGPATAPAKPPSRKR"
     gene            36568..37347
                     /locus_tag="VLE22_26975"
     CDS             36568..37347
                     /locus_tag="VLE22_26975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18125.1"
                     /translation="MDRLHEIAYGSAGAKEAEAATTLADPPGEAPPPETSVKEAESIP
                     QPEAVSETLGTSALPDSEPFSFQGKMKVEALTEATTVDSRKLAEEIAPKFVDVFVATF
                     QEMGRHASASNGAVSEVVAEMNRVSQHLQVLSGQMGELRRSIDSMTASQQELSTRVSD
                     IEAGLREHEKVYLNLEAAMTQSEQALRAQQDLLTEKLDSASAQVAQINERLDSQAGAI
                     RALHESSRNREEHRDELRSALQAIQDLASKLSTPEPLPDKL"
     gene            37478..37882
                     /locus_tag="VLE22_26980"
     CDS             37478..37882
                     /locus_tag="VLE22_26980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18126.1"
                     /translation="MFGWLKNRRETPLSGAPAVRRQKSYSAQSGFVYQYFYQGNRSAI
                     REREQGQEFVFEVTVDRKTSFPVSVFVSESVVVDWQNLHNRALSPTERYAIAKMALFQ
                     AFDDRRTPGEMREEVRVRTADVRGILDILGIE"
     gene            37898..39538
                     /locus_tag="VLE22_26985"
     CDS             37898..39538
                     /locus_tag="VLE22_26985"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB18127.1"
                     /translation="MTELLLTTADGHARSVPLEGDLVSMGRSPENHLAFPEDGLLSRR
                     HLVFKPATDGWAVQDLDSKNGTFVNGRRIEGERTLRAGDRISAGGLKFVFKGTGELTG
                     EVTFEVPPGEDSVLRTKSISLPEALSVVSSRRGRVSSGTRKAWRFIDGVRELNVPRPL
                     AELLQATLNLAMEAAEAERGVLLTFDEKDRLTAQASSGGDFRISATVRDRVLRDRVSL
                     LVEDVAKDERLLTSDVIAQSHIRSLVAVPLQTEDRVQGMIYLDTSHPTRRFNDDDLSL
                     LTVMANVASLRIERERWDLQRRALISEHITTLQKLAAALSHELNSPLGTIKSTIDTLV
                     RVSAREEAGPGARESDLASLRENLHSTLEASISRIQQVIARIQRFTDLDRAEIQSIDL
                     NELLSDTLAQLDVPEDIDVEFYFDALPPVVCRPRNLNIVFLSLLRNAVDACRQLPLKR
                     GRILVSTHAREGRVEVRIEDNGCGIRGVDREQIFDPAFRVVEGRVAAGNWGLFSARQI
                     IREQGGEIAIDSEEGRGTTLLVTLPGRVVERRMGPERD"
     gene            complement(39535..41799)
                     /locus_tag="VLE22_26990"
     CDS             complement(39535..41799)
                     /locus_tag="VLE22_26990"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HSB18128.1"
                     /translation="MAIRKEDALEYHSSPMPGKIAIVPTKPCRTQRDLSLAYTPGVAT
                     PCLEIERDPSLIYKYTGKGNLVAVVTNGTAVLGLGNIGAGAGKPVMEGKAVLFKRFAD
                     IDVFDIELATEDPKEIIRAVQLLEPTFGGINLEDIKAPECFEIEEELKRTMKIPVFHD
                     DQHGTAIISGAAMVNAVEVAGKRLDEVKVVFNGAGASGISCAEHYVRLGVKRENITMC
                     DTKGVVYKGRKEGMNKYKERFARETEARTLADALRGADVFVGLSGPGCVTPEMLQSMA
                     DDPVVFAMANPDPEIPYDVAVAARGDVIMATGRSDFPNQVNNVLGFPFIFRGALDVRA
                     TAINDDMKLAATRSLAALAKEDVPDSVRRAYGVERMEFGREYIIPKPFDHRVLIWVAS
                     AVAKAAMESGVAQAPIDIDEYKDQLERRLGKAQELMRSMIHKAQRKPQRVVFPEGEES
                     KILRACQILLDERIAAPILLGSEERIRARIDDLHLHLNGIQVVDPSNCQALPRYVEEL
                     YRGRHRKGITRSEAEELILNRNIFGSMMVRMGDADALVSGLTQHYPDTIRPALQVISV
                     RKGLRRVAGLYMLITLKGDVYFLADATVNIEPTDEDLAEIAILAAETARRFNVEPRVA
                     MLSFSNFGSTRHPLAQKVERAVQLVKQRVPALVVDGEMQADTAVTPEIIDQTYPFSSL
                     KGGANVLVFPNLESGNIAYKLLMRIGGCEAIGPILMGLSRPVHVLQRGCEVSDIVNVA
                     AIAAVDAQEMAGTG"
     gene            41923..42687
                     /locus_tag="VLE22_26995"
     CDS             41923..42687
                     /locus_tag="VLE22_26995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18129.1"
                     /translation="MSVPPIPPEFDHLSHRPFSFYPPILNIEHNEWLLRRGTWSEILV
                     VNRKTDQEVWIPRRYIGEVSRVDEPVMIVGLTKELEYKGGTVWPHERRVIEMPRAVNE
                     GVSRQPAKIPEEPAPVVGIRLEPSESRISRLVGAMLLAGVLVCVLVVVLSQRSTSFRA
                     IEQFDLGLTADDDYHSIVRKLGTPSEDRWRSETGELQYRLLRFPARPYTLILMGTDRD
                     HAHYVGALDAEWRPVNSVRLPSGNDTMAMLKKLSKW"
     gene            42734..44797
                     /locus_tag="VLE22_27000"
     CDS             42734..44797
                     /locus_tag="VLE22_27000"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HSB18130.1"
                     /translation="MKVAGYIGWGIIAIVAAYFLGGIALERGEPVNSMWLVLAAGCTF
                     LIGYRFYGKFIAAKVMALNDERATPAERLRNGHDFEPTNKWILFGHHFAAIAGPGPLV
                     GPTLAAQFGYLPGTLWIILGAVLGGAVQDFVILFCSMRRDGKSLGQMAREEIGKVGGI
                     TALLTVLLIMIILLAVVALVVVNALKGSPWGTFTIAATMPIAVFMGLYLRYWRPGKVL
                     EVSAIGFALVVASIFAGQWVAHSSTVASWFTMGGIALAAAIIIYGFLASALPVWLLLA
                     PRDYLSTFVKLGVVLMLGAGILFVRPELELPAFTRFVDGDGPIFAGKIFPFCFITIAC
                     GAISGFHSLISSGTTPKMIAKESHAWPVGYGSMLLEGFVAIMAMIAACVLQPGVFFAV
                     NSPAGIVGATPEAATATITAWGFPVTAAEMEVLARDVGERTLFHRTGGAPSLALGMAH
                     IFATGAGGKAILGFWYHFAIMFEALFILTIIDAGTRVGRFMLQDLLGHVYKPLGRTSW
                     MPGVIGTSAVVVLAWGYFLVQGVRDPFGGINSLWPLFGISNQLLAAVALCVATTVLLK
                     MHRARYMWITCVPLAWLVVVTFTAGWQKIFSPLPRVGFLAEAAQLQAALDAGAVAAAK
                     VTETNTLIFNARLDAVVCGIFLVLVSLILIDSVRLWVGILRGTREARITESPFVVSRL
                     RPEEI"
     gene            44794..44970
                     /locus_tag="VLE22_27005"
     CDS             44794..44970
                     /locus_tag="VLE22_27005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18131.1"
                     /translation="MKRLRTFARMVVGLLRELSDESAYERYLAAHGREHSREEWRRFS
                     EKRLRMKYSKPKCC"
     gene            complement(44987..45307)
                     /locus_tag="VLE22_27010"
     CDS             complement(44987..45307)
                     /locus_tag="VLE22_27010"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSB18132.1"
                     /translation="MLGSAIPSGGAMQPQAARNGQVFEHKKLTRKEAQLLGILRLNEG
                     RCLSRGYLLETIWGYRGGTRTRTLDVHIQRLRRKLGPDGASHILTVFRNGYSWRRDDE
                     PPGL"
     gene            45565..45948
                     /locus_tag="VLE22_27015"
     CDS             45565..45948
                     /locus_tag="VLE22_27015"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB18133.1"
                     /translation="MFCSTAVERRRGKRYLLRLACRVTSPDERFAEIQGATTDISRTG
                     VRVVFKDTAAVPAFRIGETVCVLMDLPQSPYFTPRVLECIGRVVRVGDTDPAGPAVAF
                     EIVRTRVRERDGRVVEGDATASLLQ"
     gene            complement(45980..46624)
                     /locus_tag="VLE22_27020"
     CDS             complement(45980..46624)
                     /locus_tag="VLE22_27020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18134.1"
                     /translation="MEEIFAVVSSSIASIGAYRSWIVALIWLLIFLIAALICLMVMRR
                     GSLAPKAPVAARIDDGLDPVEAKRQALRARHKVRPLTQLEDGFGFGKLPDGAYGFTML
                     PASRDSPVLRAKQPGLFEIHKTPEGSILIVGFTSSATAAAIATQRVRAPLFAEPVTEA
                     PLLVSIPVSSVLRFVSRDRNAVEVEVEPADSEGPVHGKPPGTAASDAAAKARSA"
     gene            46918..47757
                     /locus_tag="VLE22_27025"
     CDS             46918..47757
                     /locus_tag="VLE22_27025"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB18135.1"
                     /translation="MLGLSGLLATTCWRSGALAKPLGLPIGLQPYTVRRELAQDCEGT
                     LRKIAAIGYQELEIGDPFYGQENDGLRKLLKSLGMTTSSAHYGYPKDGSEWAKSIEHA
                     KMFGIKYMITSAPSEWRKSLDGWKRAAERFNELGEQCRRAGVAAAYHNHHFEYVVLDG
                     KVAYDEFLRLTDPKLVFMELDCFWTTFAGKDPVAYMEKYPGRFPLLHIKDLKPGFPPS
                     TDAVKGKPYAEVGKGIIDWKRIFQAAPKGGIRFYYVEQDECDRPPIESIRISHDYLNE
                     LSV"
     gene            47809..>48062
                     /locus_tag="VLE22_27030"
     CDS             47809..>48062
                     /locus_tag="VLE22_27030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18136.1"
                     /translation="MTPSRILVVFTLVAVMAGITLAADVTGKWTGQVPGRDGQMREVT
                     YTFKVDGETLTGSMTGFRGQDLPISDGKISGDTLSFVVKA"
BASE COUNT         9387 a        14387 c        15034 g         9254 t
ORIGIN      
        1 ctcgccggga caaaggagcg gctcactgcc gcaggcgttc gcatcactcg tgagatgccg
       61 gagcagagcc agttcttcgc cgagtttccc gacggcgtgc ttgttgagtt caccggcgat
      121 ccgaaactcg acgcgcccat caagcaccac cacctccacc ttgcgtcaaa ccagatcgac
      181 gagatgcgcg cgtggtatgc gaaggtgttt ggcgcggtgc cgggcatgcg aggcaagttc
      241 aaggctgccg acctgcccgg cgtcaatctt tcgtggaaga cggccgagaa accgccggtc
      301 cccaccaagg gccgccggct cgaccacatc ggctttgagg tgacggacat caaggcgttc
      361 tgcgcgaagc tcgaagcggg cggtatcaag cttgacatgc ctgttactcc gcgccccgac
      421 ctcggcttgg tgattgcctt catcacggac ccgtggggca cgcgcatcga gctgactgag
      481 gggctctcac ggctcagcgc cgggaattga ctactatcag tgcaacggcg cccgccatgc
      541 catgctgcct atttcatcct caggatgaag aacagcggcg ccaacacgat caccagcatc
      601 aacgccatca ggccccacga ccgcctgttt ccgaaattca gtgtgtagcc gagaccgcac
      661 ctcttttcga ccatcagcga cggatccgcg ggattgtagt aaaacacgcc gagcttccag
      721 caccccgccg gtgtgccgtc gccctcctcg gatggctcgg tgctcattcg catcaacgga
      781 atcgcgaatc cggcaacggt gagaagcagc ccggcgacgg aggcccagat gagccgagcg
      841 gcgatcggct gtggaatgac cggaagcagc gcggctacgg tcgtcaggaa cgagaccatc
      901 cacattacga ccagaagcat attcacattg aggcggtggc gccgcacggc cgtctctccc
      961 cgcaaaggcc gcgagctgta ctggatcatc agcaggatcg ccagcatcag cgccagtgag
     1021 ccggctgcga tcgccggaac catgaacacc gcgccgggcg acttcccgac ccagcggtcc
     1081 gcctgcgcca ccgcgttgaa gtggacggga aatcgagccg ggagctggtc atagtgcgac
     1141 caaagaaaat acgccgcgcc ggccgggccg agaaacggga ggactgccag gaacatgcgg
     1201 ttcattccac caccttgtcc ttccgcacta ttatggggct tcaggacgct ccacgatgcc
     1261 ctcacggtat ctgtcagcct ggcaagcgca ctcccgccgc gggtttctaa gcgcgctcgg
     1321 cggcgcagca atgctgcgcc caatggcttg cgccccgcaa ttcgccacgc gcggtgtcgt
     1381 cctgattccg gaagacctga cacttccgga ctggcctgac cgagcgaagc agtccggcct
     1441 gacgaccgtg gcgcttcatc acggacgctc cgtcttcgaa gtgatcaagt ccgtcacttc
     1501 gggcgccggc caggaattcc tcgcccgctc gcgccgactc aggctggagg tggagtatga
     1561 actccacgcg atgagcgaac tgcttccccg ctggatgttc gaagaggaca agagcctgtt
     1621 tcgaatgaac gaaaaaggcg agcgcgtacc cgactggaac ctgtgtgtcc actcgacgcg
     1681 cgctcttgag gtggcgtcgg agaacgctgt agccgtcgcg cgtctgctgc ggccgaccac
     1741 cagccgctac ttctactggg gcgacgacgg aaggccgggc tgccgttgcg gaaagtgccg
     1801 ggagttgtcc gatggcgaac aggcgctgct gctggaaaac cacatggcga gggcgctacg
     1861 ccgccacgac tcgcgcgcgc aactcgccca tctggcgtac agcacgacgc tggctgctcc
     1921 gcgccaggtg aagcccgagc caaacgtgtt cctcgagtat gcaccgatcg cccggcgtta
     1981 cgacattccc tattcggaac agatggaggg tcgagacggc ctcgcgaatc tcgacgccaa
     2041 cctgcgggtg tttccggcgg caaccgcaca ggtcctggaa tattggctgg acgtatcgcg
     2101 cttctcccgc tggaagcgcc cggcagcgaa attgccttgg aaccgcgatg tcttcctggc
     2161 ggatgtgcgc acatacgcgg cacgcggaat tcgtcatatc acttcattcg cctgctacat
     2221 cgatgcagag tacgtcagaa ggcacggcga gccgtgggcc atttccgaat atggcgccgg
     2281 actccgccag agaatgtagg gattgcagtg agcggccaga ccgttggaag ctcttatcgg
     2341 tcaggggaag tcagctccag gaaccgggcc tcttctttcg cgcgctgctg cgccttgatc
     2401 tgagcggtgg cggcgaatgc ctcccgcgcc tcggccagac gacctacctc tttgtaccag
     2461 gtcgccagac ggtaatgcca cgacccgtcc gggtcagcag ctatcgcatg cttcatctcc
     2521 gcaatagccg gagcgaactc gccggatttc gcatacgcat cggcaaggcc cgcatgcgcc
     2581 ttggcaaggt caggctcccg ctcgagcgcc cgccggaaac gtcccgcagc cgcgcctgcg
     2641 ttatcgctct ttagaagctg cattccgagt tccacgttca actcctcatt atcggaacgg
     2701 tccgccgcct gttgcgtgta ctccagggcc gccgcggggt cgcgtggagc aatgaaccgg
     2761 atgaagagca actgcgcctc gacgttgtcc ggagccagcc ttgccgcctt ctcgtacgcg
     2821 gcttgcgccg cagcgctgtc gccggcgttc ttcgccaggt ccgctagaag caggcaggcg
     2881 tggagcgaat tcggattgcg ccggacgctt tcgaccagta tttccctagc gagcgcatag
     2941 caggttaggg agagccaata tcgctcgcgc tgcgtgtcgg acggcaaaag cgactgaaat
     3001 acctcctcgt agctaccatc acgccaaaga gcgagtgcag gcgccagtgg atcgggggca
     3061 gagtcaggtt ccatatcgct gattgcctcc gccttttcag gctggttacg gcgtaggtag
     3121 aacgcccgca acgcaccgcg cacctcccct gactcgggca aggcctcaag ggccgcctgg
     3181 tagttcgcct ctgcggcttc gttcgcatct ccgatcgcga gatactctgc cagtaacagc
     3241 tttccatacc ccgaacccga atcaatcaac ttccgcgcaa tcgatttgct gaaattgagg
     3301 tacgcgatcg ccagtgcgta gcgggcctcc tccattcccg gctggcgtga caagacgccc
     3361 cggtacagcg cgatcgcctc ttcgaaatgc ccaagtttcg cttgtgcatc ggcgagcgca
     3421 tagcccgtct gagcgtttgc gggatcgagc ttcaacgcgg cgccgagcgg tgccagggct
     3481 ttccggggtt cctcgaaccg taggtattca agccccagaa agagctgtgg cgacgtcaac
     3541 tgcggcttca actccaccgc cttctcgaag gcgtgaagcg cctcccggtg gcggccgagc
     3601 tcgtatagaa ccagtccctg gttcgcccaa acttccgcaa tggttgcgtc cattctgagg
     3661 acagcgctgt acttctcgag cgccccggcg aaatcccgcc gctgctcggc ctggcgggct
     3721 tcacggaaga gcgttggaat atcctcggcg ccgcccccga gcgcggccgc tacaacgaga
     3781 actgcgatca tggctgtgcg aagtccggta cttccacgtg gctcttgagg tcggcaagcg
     3841 cccggaaccg cgcaagctgt gcgctcgctt tctcggcctc gccgagccag cgatacacga
     3901 gcccaagctg gtagtgatac tcggaccggc tctcatccag tttcacagct tcctccagct
     3961 ccttgcgcgc cgctcgaagg tcgccgatct ctgccagctc ctttccgagg tgaaagtgcg
     4021 gccgcgccca atccggccgc gcttccaccg cccggcggaa gagcgccagc gcctgctcgg
     4081 gagcgttacg tgtccggtac aggaagccga gttgcagtag cggccaggcg cactcacgac
     4141 cgttttcaca ggccgacagc gcctgtttgt aactggtttc ggctgcttct accttcccga
     4201 gccgttcttg cgtctgcccc aacgccgtcc agcccttgac cgaggcgcgg ttcagatcga
     4261 cggcacgctg cagggcatca tgtgcttccc ggaatctccg caactcgaag agcgtgcggc
     4321 cctggtaata atggccctcc caatcgccgg gctggagagc gacggctttg gcgaaagcct
     4381 tttccgattc gcgcaaaacc ccgccgagcg cgtaatccag cccgagaatc ttgtaaattt
     4441 ccgcgtggtc tgggtgccgc cgagccgctt gctccaacca cgagatcgaa tcgttcaggc
     4501 ggcccaaccg catcgtagcc actgcgagca gcatcaaccc gtccaacgat tcccgctggc
     4561 gtatcgcggc agaggccgat cgttcggcca gttcgtaccg ccccgtccgg gcgtaaattt
     4621 gcgcgatcag gagatgcccg gtgctccact ctggcagtcg ccgcagttct tcagcgatcg
     4681 ctcgctccgc gcccgagtaa ttccgagcgt tgagcatttc ccggatcctt tggggaaccg
     4741 ggacgtcctg ggacaaggca atggccgcaa gaaacatcgc gcacagcgcc agcgcaactg
     4801 ctcttgcggc catcaacccg atccctcccg agaggaaacg gctccctcgc acatccggga
     4861 cccgccgtac atcagaacac aaagcgtatt tgggcttgca tctgccgttc gaggtttgaa
     4921 cggccccaga acgacccaaa gccaatagcc cggccgccct ggattcgctg cgtattggtt
     4981 acgcccgtgt cgggattgtt ccagatgctc cggttgaagg cgttgagagc atccattcgc
     5041 agctccagcg tcgtaccttc tgtgaagtgt atctgtttag acagcgcgag gtcctcgttg
     5101 acgtaaggga agccgcggat tgcactgtag gcccgcgctg cattgccaac cgtgtaggca
     5161 tcgggtgctt tgaaggcgtt gatattgaac atccacgcct gtcccggcca aacgctgtcg
     5221 accttgttct ccagcgactg gccggaaaca atatcgtagc gtccggcatt gttgaagatc
     5281 acatttccca aattgttggg atagcccatg ccaagcgcgc tgccactggc gtaattcagg
     5341 atgcccgcga ttttccaccc gccgattaca tgcttcgccg cagcattggt taccaggttg
     5401 ccgagcggta gttcgtaacc gaacgccacg gccagattgt gcgtcctgtc gccgggattg
     5461 ttggatgcag tggccagagc ccgttcgagc gacagcttct tcgcgtctac ggatcccgca
     5521 ccgccccacc caaaggcatc atctactgtc gtgatcgctt tcgacagggt gtacgcgacc
     5581 agaaaatcaa ctcccttcga gagacgctga gtcgctttga cctggagcga gttgtagtcg
     5641 ctctcgccaa ctgtcgcctg cggatcgccg atcccgcggt actgcggaaa tggacgtagc
     5701 gcctgcgcca ccgtacctga gaatccctcg tacgggtacg tcacgccgag ggcttgggcg
     5761 tcggcggcgt tgttgatctg gcgaagcagc ttatcgccga gggaaagcac gccgaagtcg
     5821 ttctggttca ggttgacgaa attgccggta atcagccgcg tgccgtggtt gcctacatac
     5881 gctgcctcaa cggtcaggtt cggggtgatc tggcgctgga cggtgaagtt ccagttatag
     5941 atccgtggaa gtctgccgct ctccggccgg tatgccgcga gtccctggtt gttcagaagc
     6001 gtgacatcaa agaccggagg aacgacggtg accggcggaa atccgctatc cagcgagaat
     6061 tgtgcctgag ttgccggatc gatggcggga tactgcgcgt tcacgctgcc gacaccattg
     6121 gactggatcg ggtcgaaggt gttgacctcg agccgcccgt aaacaacccc aaatccgccg
     6181 cgaaccacgg tcttgtcgct gaccctgtat gcggcgccga tgcgcggtcc gaaatccctc
     6241 ttatccgtat cgagccaact gtcgcggccc gtgcggccag gctccgatcc tgagcccagg
     6301 aacccgagcg cgccgggccg cccgcctgca ccggtattgg gcagggtggg aacgaacgac
     6361 gcaatgcggc cgtatttctc ggtcaaaggg cggtgcagat cataccgcag gccgtagttg
     6421 acggtcagtc tgggagtgat cttgaaatcg tcctgggcat agaacgcata gtcggagtac
     6481 cggtaagagt aatgtccagc cgagttcagg tagctgcccg ggcccgcctg gccgagcaga
     6541 aagctcgcga aggcgctgcc gagcaggctg taattccccg aactgcccga taggtaaggg
     6601 agcgcggtct gggcagcatt gaatgacaat ccgccgtacg cgccgttgcc gccgaagaag
     6661 ttcgcgttgg acttccggta ttcgacgccg aacttcaacg tgtgcgagcc gaccgtcttc
     6721 gacagagagt cgacgacggt gggcacggtg tggtagaggt gcttcaattg ttgcgtgccg
     6781 aaagcgctca gaccgtcgat gttgaccgcc gggaaccacc cctttagcag tgtgcccttc
     6841 aaacccaatt gggcctccgg atcgaagccg cggtcaggca cgccatccgg attctggtac
     6901 cgcagcaggc cgacggtgaa gtggttcagg gcggtgctcc cgaacgtgta atcgtgattc
     6961 aggcgggcaa tcttggacgt gatcgtctgg tcgcgcgcgg ggtcggccgc gccgaacgga
     7021 gtcgcgccgt tgatcttgtg ggtgtacatg tagttaaacg agcccgagag gcggcttttc
     7081 ggcgagaagt catgatcgat cttgagactc cagatgatct catcgcgcaa tggtgtgcca
     7141 gcaatcccga gcaggttgtt gctgtcgagg ccggcgatgc ccgctggcgg caggaagctc
     7201 aggatcttct tggcgacctc gctgcgcaac gggacctggt tattcgcaaa cggatcgcgc
     7261 acgacgtagc tctgtccgtt caccacctcc gttcgtgttg tcgcggggtc gtagatctgc
     7321 ccctgctgaa tcggacgtcc tagcacgtcg tttccgagaa cgttccctgt cagcaaggag
     7381 gagagatcac cgtttcgcca ctcactgcgt ggaacgtcgt agaagccctg ctgcttttcg
     7441 cggtcgtagt attcctcgta agaggtaaac cagaaagtcc tgttcttccc gttatagagc
     7501 ttggggaata ccaccgggcc gccgatcgtg ccgccgaatt catgccgcct gagcctggtc
     7561 ttttcgccgc tatctttgtt tctggcgttc aagaactcat tccgcaggta ttcgtacgca
     7621 gtgccgtgca ggttgttcgt gccggactta agtgtgaaat tcatgatcgc gccgccggcc
     7681 tggccgtatt ccgcattgaa gttggcggtc tgaacgctga tctcctggat agcgtccacc
     7741 gaaggttgcg cctcgttgaa gacgccgggc gtggggttgg tgctggaggc accatcaatc
     7801 cggatctcct cagagaagct ctggagaccg ttctggctgg taaccggcca gctctggtcg
     7861 cggcttacgg acgagcccgg aaccagccgg aggaagctca ttgggttgcg catcgcaccg
     7921 ccgaaatcga ttggcagggc gctgagcttc tcctgcgtta ccgaagtgga gacctcagcg
     7981 ctttccgttt tcagcatgct ggcatcagca tgtacctcaa tagtctccgt cagttggcct
     8041 acctgcaggg aaacattctg cgtcacggtc tgggtggcgc ccaccgtaat gccgttctgc
     8101 acgtaggctc tgaatccggt gtgcttgacc gacatgttat acgtgcccgc aggcaactcc
     8161 tggaacacgt ataccccggt ttcgcttgtc agtgcttcac gcgcggtgtt ggtggcggcg
     8221 ttcgtcaccg tgaccgtcac gcgcgggaca ggagcggagg tcggatcggt gaccagaccg
     8281 gtcaacgagc cgcggtcgct ctgtccgtaa gaacatactc cgagtgccag catcagagcg
     8341 gcgatgcata ctataggcac ccacttcgat tgcatggaac ctctccttat tctgccgcga
     8401 tgttcgctat cgcttagcga cggctatttg tgtaaaccaa cagcccacac cggctggccc
     8461 cggattggag cgcgtttacc tcgatgtcgt gatactcact ccagaaccag gcggtgcttt
     8521 caaaaaagct acctcgcgat ctttggattg ccagatcgca gaccttccag aacttctttt
     8581 gtcctgagtt gccgaagcga ggaactctaa gtcgttccgg ttgaattctg gcacatagtg
     8641 gcggatggag gtaggttgaa gcaccggtct cgactgctta ggcgcttcgg ctcgaaccag
     8701 gggtaggacc acccccagtc ctggcaggac tttacacact gacatcggct tcaacatctt
     8761 tccgcagtaa gcgggcaaga gcagcctcga gggtgaccta gctagcatgc tccggaagag
     8821 cggcgaacac aagaggggct gtggttaaat tttataaatg taataaatat agtattttca
     8881 gtcaggtacg ttgaagggtg agcacccgaa accggaagtg gattgccgtc gtatttcttc
     8941 catcccgctc gggccttttc gcgcggaacc acttcaacct cggcgacgag ccgatctctt
     9001 caccgtcggc gaacgcgtgt acccccgctc caatgccgct actttcgagt tctttgtccg
     9061 acgacttgaa gaacgggata ttctgctcca gccaggccct ggccgcgttg ttatcgatgc
     9121 agttagtctt ccgctctgcg tcattcccgt ttgaattcct ccacatagta ccggaaagcg
     9181 ccgggctgca agcaccagtt tggattgcct gggcgctccg gccaggacca gtggcaggct
     9241 cactaccaag gccagcaggg cttcagcgga tgacgtcggt ttccttcagt gtgccgagcc
     9301 cgaaattgtc acgaggacct gccggctccc caactccatt agtaatgtcg cagggacggt
     9361 ggttgcgctc ggtaacccgc gtacgccttg ctccagctct ccgtcatggt cccgctcgga
     9421 aacttcttgg agtcgccggc ggaatcggtg ctgcgtttag gtaatgctgg ctgcaacgcg
     9481 cgattgtagt gaacaaaaag cccgcagtac cggcacaatc gcggaaacag ttcatctaga
     9541 aggtcggcgg cgtctggaag cgtcttgcgg ccgatatcca tcccgaagac gcggtcaatg
     9601 ccgccgcaaa tcgggtgagg gtagtagccg tagcgggtga gtcccagccc gtacatcgac
     9661 ggcaggaaac agccctgtga aaagtcgtac ttcgaatgcc cgggaaggtc gatgggcgcg
     9721 acgttgaagg tcagatgccc tggctggact acctgagtct tttccgagca gtagacgatc
     9781 actgccggtg ggagctgtcg caccatgccg cggctgaact gggaatatcc ggcggtgttg
     9841 acttggataa ctgtggacgg cgagtctcgt cttacgtaac tgtgcagcgt gttgacgatc
     9901 tccagaaact gcggatggcg agtgggttca cctccctcaa tctggattgc gttccaatac
     9961 cgatgctgaa gtttgctctc gctaataaag cgattcacct gatcaacggt catgtgctcg
    10021 ggtgcgggcg cctgccccgc gccacaggac cggttgcagt ccatgcagcc gaggtcgcag
    10081 gatgccgtga tgtcgatgat tatacgtttg tgggacgggc ggtacggggg gcccgatcgc
    10141 aaggcggcgc gcaccgccaa atcgtgcttc caacggaaaa ctcggatggg ccagtgctcc
    10201 gccgccagtt gcaaggcggg gggcagcttc agaataaggc tctgcagtct cattggcgca
    10261 cggtcgtttg cccttgattc cggatttccg aaatgaggcc gaaatactca accaagtggc
    10321 cgtcctcaga ccataccgcg ggcgtcctcg ccgtaccgcc gtcggcgcaa gcacccaact
    10381 ccccactgct ttgcgagatc tcccgcaaga tgttcagcag acccttcgct ccgtcaaaca
    10441 tcattacgcg cgacgaggtg gcggcgacct cgggaatcgc acccacgcag cgggcaatga
    10501 caggcgcgcc cagggccaga gcttccgcca acaccaatcc gaaggtctct gggaacagtg
    10561 acggcaccac cacggcccgg gcgtgctgaa acagcaaccg cacttcatcg ttgggcagga
    10621 gccccacaaa cttgacattc ggcatgccgc tggcgatcag ctcaagctcg gtcctcagcg
    10681 gaccatcccc ggcgatcacc aggtcgaggc cgggcacctg acgcatcagt ggaatgacgg
    10741 tctgaaagcc tttctcgaca accaaacgcc cggccgcagc gatgaatggt cgtgccggca
    10801 gaagaccagg cgcgagacct gcgggggcct ggccgcgcga ttcccagttt gctggtagca
    10861 gcccaggcag gacgcgaaga cggtggtttt tcacacctgc ggcctcgtag atccgcatgg
    10921 tgctggtgct cggaaacagg atcgcgtcta gaccggagaa cgcatcgttg acccagccag
    10981 ctcgcctcca gagttgtggt ggcactcctc gccgcagatt acaggtgaaa cacgcacgag
    11041 cctcgcacac gctgcggccg aacttccaca gtagactttg tgggcagatc caccagtagt
    11101 cgtgcgccgt tagtaatttg accgctctgg ataacttgga ggccactcgt ggcacgccga
    11161 gaccccccag cagagagatg ttgtggagat ggatcacatc aaatgcttca tgctcaaaaa
    11221 tccaccgtag agtggctgcg ttacgcccgg ctcccgcggt ctgatggctc cacagggcgt
    11281 ccaacttccc tctccagccg gtgcccagcc ggtgctcgat cagcccgtcc ggcggctcgt
    11341 acgatcgcag cggcgttgat ccccggacgc tctcgaatgc accagggctg cgaacgacgt
    11401 gcacctcatg tcctcgcctt aacagggccc ggcacaaccg ctcgacgtag accgagtcgc
    11461 caccaaagct ctgcgcaccg aaaaacgatg tgaccatgca gaacttcatc tgatgctgcc
    11521 gctttccact agccttcgcg tgagcaatcg cgcggcgccc cacgtttctc ccaccgccca
    11581 caactcatgg cctaccacct ggatcggcag cgtcagaaag aaggccagcc cccagcccgc
    11641 tctgagtgca tccaatgcgg cgcgaccgag aacccaccca atcgcagccc aactgaacaa
    11701 caaacgaagc catgtgatca cgcccggcgg gtccatcagc tccgtttgcg ccaccgcgaa
    11761 gtcgcggccg cgttggaacc gttgtcggca aaacccacta aagccttcaa gatggcggtg
    11821 caggacaata gcgtctggca agaatcgcgg cggctgcccg cacttggcgg ctctccaact
    11881 cacaatcccg tctccggcga acaggtgacc gggaaagggt ccgatatcct tccaaagctc
    11941 acgggaatag gccgcattcg ccgtgggcgc gcaacgcttc acaccaggcc tcaatccggg
    12001 taataggcca tggaacttac ataagtgaac acccgcttcc caccacgctc ggcggccgag
    12061 ccccatcgcg ccgacgatcg caccgtcgcc ggaatcagac gccgttgcca gacaactgag
    12121 ccagtcgcgg ctagcgacgc aatcgggatc agtaaagacg agcagttcgc ccctggcctg
    12181 ttgtagcgcg agattccgag cggcgtgcgg cagcagcctc gttgggctct ggacgaatcg
    12241 cgcctcagga taaagcgcac gtacgatccg ttccgtctcg ctttcctgtg aagagttgac
    12301 cacgatcaat tcgaagtcac ggaatgtttg cccacgcaac ccgtccaagc aatccgctat
    12361 ggtcgaagag gagtggtagg cgggtatgac gaccgaggcg cggggcgctg atttcctgtt
    12421 catctacctg cccggggcgc cgacaactca agccaaataa ggcgcgacgc ggagagttcg
    12481 ccactgcgca ggagccccca agcctctccg aagcccccgc atacgaatag gagtaggatc
    12541 acaggcagac ccgctagcgc gtcacgcagc aacggacggc cgcgcagggc gctgaagagc
    12601 cgctttagcc gcagtgctgg aaccaacagt ggcaccgcca ttgcggccac gattcgcttc
    12661 gggagattcc acgtttcgtg tcgcaagcgg cggttggcga agaaccgcgc gtactcgaag
    12721 tggccgacca tcaagtcccg caaagtcgtg ttggtctggt gggcaaggag agctcgcggg
    12781 acagacagga ctcggaagtc ccgacccatg aaccggtgca agaagaaatc catctcgagc
    12841 agtgattcca gcctctcgcc gagagccaac agcacatcgc gccggtacgc gatgttgttc
    12901 gccgttgccg tcggcgcggg tccatccggc aacggatgcg ccaacgcacc gtactcggcc
    12961 atgaacacag atctgtagaa ataggtgtcg gggctgccgt tcgtgaacgc ataacacgcg
    13021 gcggcgacat ccctatcctt ccggacgaaa gctcggtcca cctcctctgc ccaacgggcc
    13081 gtgggtagag tgtggtcctc aaggaaggcg accacaggcg cctgagcttg ccggacgccg
    13141 tcggcgcggg cggcacccca atcgtcactc ccaggccgac gcagaatcga agctctcaac
    13201 cccgtcggcg ggatgatgtc tggcaacgtt tcggatccca cgtcaatgag gatggcttcg
    13261 atatcggagg tgatagtctg accggccagc gccgccagca gaccatctgc cctccgccga
    13321 tcctggccgg tgatcgccac aacagacagt gatggtggcc tcaagccgaa acctcaagcc
    13381 gccgaatcgg cggtctccct ggggagccgt ccgcccgact ccccgaagcc aacagtatac
    13441 ctttcaggtg ttcggctccc ttccatactc gtgaaatatc caggctgggg atggggattg
    13501 gaccgctgtc tcatttgccc gcttgttccg gcgggacaac cggcgcgtgg cgggggatct
    13561 tggagcggaa gtcgccgagg cttctccagc gggtgtgctc gttgaggtcg acctcggcga
    13621 gaatcaccgt accgaagtcc ttggcggatg cgatcgttcg tccggcgtgg tcccacacgg
    13681 ccgtgagcat ccagttgctc gaaatgtctt cgtacgtgct gctgactaaa tacgtgtggt
    13741 tctcggcggc gcgggcgcgt gcgagttcgg gattgcatcc ccatacaggc cacgcgatca
    13801 cttccgcgcc acgcttggtc agctctcggg cgacttctgg gaagaagccg tcatagcaga
    13861 tcatgatgcc gagcttgccg aaacgtgtct ggaacaccgg gtactcgtgc cccggtgcga
    13921 tgcccgcctc gacttctccg tcgggaaggg tgatcttgcg gtatttcccg gcgagtgagc
    13981 cgtcgggagc aagaagcgcc gcgacgttgt gtaccaggtg cccgtttcgc tccaccagcc
    14041 cggcgacaat gtacaggttg tgctttttcg ccagctcgcc gaagtattcc gtcgaaggcc
    14101 cgggaaccgg ctcagcgacc tcggcaaacg tcttacccgt accgtaaaaa gtcagcgtct
    14161 caggcagcac cacgaggtct gctccccggc gtgcagcctc ttcgatcagg ggcgcgaaca
    14221 gccggcggtt tctttcaggg ctcttgccct ctcgtggtcg gaagtggatg gaagccaagc
    14281 gaacctttcg cggggaagga ggcgagactg cggtgaacga cacgtcgctc cagagaatcc
    14341 gtcctttcgg cgcccactgg aggtgcagtt cgacgacggc ctggcgcgca ccgccgggcg
    14401 cctgatgggt gtcggagacc tcggtccagc cggccgcatc ggtctgtttg tcagtgggga
    14461 actcgggggg agcggtagcg ttgaaattgt tcaggtaccg ctccacaagc ttgcgatcgt
    14521 ccttcacttc ttcgccccga tcgtcgagcc acttgatccg gacgagcgcg ctctgccggg
    14581 gcacggcgac gccggtggtc ttgcgatacg cgagaaagct gtagtacttg ccgccctcga
    14641 ccgaaaacga ccggaaccag tacccgtcga ggccttcgcg cccgtccgcc gcgatcacga
    14701 acgcgccgtg ggccgaccgg cccgctttcg gttcatagga aaagagaggc ttgatttcgt
    14761 cgcgcggagc ggcggtcttc cagccttccg gagcgcccac cgggccggac gccgccggcg
    14821 agggtgacac gactgcgctg gcggcaaacg cgatcactgc gatgaaggaa acggtatgag
    14881 tcacggcgat tcctattgta cccggctgcc gtggatgctc accgcgccac cgggatccgg
    14941 tccaggatct cccgcgcgtc gtcgatcaga tcatctcact taggagttct ttgcgcggct
    15001 gcgggatgac gaccttgttg accagcaagc ctttttttgc gataacctgt gcgcctgact
    15061 ccacggcgct gcgcaccgcc gccacgttgc cggtgagtgt gacaaaggcc ttgcccccga
    15121 gcgccatggc gagccgcact tcgatcagct tcacgttcgc cgattttgcg gccgcatccg
    15181 ctccttcgat caacgaggcg accgaaaagg actcgaggat gcccagcgct tccagtgtct
    15241 ccactttgga gcttccgctg atggctggaa acacatcctc atgaacgttg gggatcacaa
    15301 aagtatcgat gacggagaag cttcccgcgc ggctgccggc atcgacgctc gcctggaccg
    15361 cggcgacgtc gccacgcacc atcaccatat acttgccgga gcagatggac cgcgccagga
    15421 ccaggtccac gtcggcggtc ttcagcatcg cgtccgtcac ttcatagccc gccgcgatgc
    15481 tgccgagttc gatcaagccg atgctgtttc tcgccatgtt cgtttacgct cgaatctcga
    15541 cgaacgtctc ggtgactcgc gtcaccttgc cgccgatgct cgagtgaatg tttactccca
    15601 tgtccggcgc ttcgaccttt ccgatcactg ccccggcatc gaccgcgtca ctttcctcca
    15661 ccgccggttt cgccggcttg ccggcgtgct gggaaagctt gatgcgaacc gatttcggct
    15721 ggtagtcgag agcctcatac ggcgtctccg cttcgtattc ctcgaccttc agccgcttcc
    15781 ttaactgggc cagcgggacc cgccggtatt ccttcatcgg gtggaccgag acttccttct
    15841 cttggacaaa cttgatcccc gcggcgcgca tgtctacctt cgcctggtcg cacgcttcct
    15901 tcgggaacag gtcttcggga caggcgtaga gggtgcagag gccgcacgcg cagcagagtt
    15961 gcgcccactg gttccagatg tccgccccgg tcgatgtgaa cccgaggctg cgcatcacct
    16021 tgtgcggctg cacctcgtag ccgagcaggt agcgggggca gaattcggtg caatagctgc
    16081 actggtcgca ggccgacttg ccgatgtggt tcatcgactc gatcggacgg tccttgcgcg
    16141 tgatcaggta gtggtcgcgc ggcagaacaa taagtcctgc tgtggtccga gtgacgacat
    16201 cttctagatc gaacgacagg aagcccatca tcatgccgtt cacgaacatc ccgaactccg
    16261 cgatccgggc gcctcccgcg agctcgatca attcgcggaa cgacgtgccg atgggcgccc
    16321 aaaacgactt tggctcccgc actgcgcccg cgatcgaaac gaactttttc gtcaccggaa
    16381 cgcccttgtt cgcgaagtga acgttataca atgtctcgac gttgttcacg acgcagccaa
    16441 cctgaagggg aatgccggcc ggcggaatca ggcgtccggt ggccgtgtag acgagttcgt
    16501 actcgtcgcc cgacgggtag aaatcgccaa ggaacacaag gtcggttttc tccggcttca
    16561 ggtgattgcg aaccgcctcg accgccgcgg cgttcttctc cttaataccg aacttgccac
    16621 gtgaggcccc ggtcgcctta gtcatgaggt ccatcccggc gagaatttcg gccgggaagt
    16681 gcgtcatcag ctcgacatcc ttgtggatga gaggctcgca ctcggcgccg ttggcgatca
    16741 tgaactcgac ctccgagttc gcttttatgt acgtgggaaa gccggctcca cccgcgccga
    16801 cgacgccgca ctcccggagt tttttgtcga gccccatcga gttggccagg acgccgccgt
    16861 tacgaggcgg ccggaatttg ctcaagcatc gtattgtact gcgcgaggtc gtgcttcata
    16921 acggtgatga gcgttttttc gagatcttca ccggttgtga tcatccggac caaatgcagc
    16981 gccgccagcc gggcgcacca gggatctgtg accgggcgcc cggtttgacg ggccagttca
    17041 agcaagtcgg gatgccgcag cgcgatcact ttctcgacct tcatatcgcc tgacgacagc
    17101 aggaatttca cgtccaccga atcgctgtgg cggatggaga tggcggtctg aagccagagg
    17161 aaatcgactt gccaggttcg cccaaacggg tccggcccag cctggaaatg ccggaaattt
    17221 cggatcattt gccccatcgt agcacggggc cgagggtcgc cgatcagaac tgcatgccgc
    17281 tatgagagac gagcgataga aactcgtcgc gcgtttcctt cctgtggcgg aacgccccca
    17341 gcatggcgct ggtcacggcg ccggagtgct gcttttcgac gcctcgcatc atcatgcaaa
    17401 aatgcttcgc ctcggcgatg acgccgacgc cgagcggctc gatcgtctcc atgatggtct
    17461 gtgccacctg ctgcgtcagc cgctcttgaa gctggagccg gcgggcatac atgtcgacga
    17521 tgcgggggat tttgctgagg ccgatcactt tgttcttcgg aagataggca acgtgaatct
    17581 tgccgaaaaa gggaagcagg tggtgctcgc acaggctgaa gaactcgatg tccttcacga
    17641 cgaccatttc gtcgtatttg acggcgtaaa gtgccccgtt caggagcttt tcgacgttca
    17701 tgtggtagcc gctggtgagg aagcggagcg cagcttccgc gcgatgcggc gttttcagga
    17761 gtccatcccg ggacggatcc tcgccaagtt ccagaaggat tctctcgaca tttgcggcga
    17821 tttcaccgct atgatgcctc ggctgcatca cctcgacgac tgcctttcgc tttttcatga
    17881 cttcccgttc acttcgaaga tgtttcgttt cgtctcgtag attcgaattc gctcgagccc
    17941 agtcccgccg ccgggaaacg tgtcgtccca tccggcgagc aaccggtcgc ggatcacaat
    18001 cgcaaggttc tccgtggttg gcggcgttgc ggcgaattca gccgcctgcc ggttcaggtc
    18061 cttgtgatcg aacgcgtcga caacccgctc acggaccagc gcgtccagcg cctgaagatc
    18121 gaccactagc ccggtcgctt ggtcgagcgg gccccgcacg ctgacttcga ccgtgtagtt
    18181 gtgcccgtga ccaaaggggt tgttgcattt cccgtacacg tcgcgatttt cgttgtccga
    18241 aagctgcgcg gcgtgcagac ggtgcgcgga cgagaaccgg taccggcgcg tcagtcgaat
    18301 caatgcgttc ccccgaaata gtcgacgtag aggtcttccg tctcgtatac gcggatcgag
    18361 tacaaccgcg cagccggccc acggaagtgc ggctcgatcc ggttccagat ctcgacggcg
    18421 acgttctcgg tcgtcggcac gacacggtcg aaaggtggca cctcccggtt taggaaacgg
    18481 tggtcgaagg gctccaccac ttctcggttg aggacctctt tgagccgttt gaggtcgaat
    18541 accatgccgg ttaccgggtc ggggctgccc tccagggtaa cctccaggac gtaattatgc
    18601 ccgtgaccgt gcgggttcgc ggccgctcca tacaacgatt tgttttcctc gtcggtcagg
    18661 ctcgcattgc gacagacatg agaggccgaa aactcgacct tacgcgtgat gaacatcttt
    18721 aacgtttatg atgccgcaaa gacaggtgag gatactctaa aattataggg agaacggatg
    18781 caaacggtca aaactgagcg ggtcctccgc atactcatcg gaattttgct cgtggtactc
    18841 ggctacgtga tcgtcgactc cttcgaggag cggatcgtgg tcgtcggcga cacagccccg
    18901 gatttcacgg ttaccactga cagcggccgg caggtcacgc ggtccgattt cggcggcaag
    18961 ctgctggtgc tgaacttctg ggcgacctgg tgccctccct gtgtggaaga gatgccgtca
    19021 ctcgatcagt ttcaaaagcg gctggctgga gacggcgtag tcgtgctggg cgtcagcgtg
    19081 gacaagagcg agcaggcgta ccggcagttc ctccggaagg cgaaagtctc gttcctgacc
    19141 gcgcggaatc cctccgccga gatcagcgcc gagtatggga cattcaagta tcccgaaacg
    19201 tacttgatcg acggccgggg caaagtcgtc ctgaaacaca tcggcccccg agactggtcc
    19261 gacgaacggc tcataaacga gataagatca ctgctctagg acatcatgat ctccgagcag
    19321 gcaaagcgag agctggccga agtggttggg ccacgcggct tcctggaccg gcctgaggat
    19381 ctcacgctct acgaatacga cgcgagcgtc gagaaggccc ggcctgacct cgtcgttttt
    19441 ccccgttcca ccgatgaagt tgtccggatc gtcaagatcg cgtctcggca tggggtaccg
    19501 ctggttggac gtggcgcggg aacggggctt agcggcgggg ccatccccgt caacggcggc
    19561 gtaatcgtgt ccttcgcccg gatggaccgc atcctcgaga ttgatcttga gaacgaacgg
    19621 gccgttgtcg aggcgggggt cgtcaacctg gacattacga tcgccgtaca gggccagaag
    19681 tacttcttcg cgccggatcc ctccagccag aaggcctgca ctattggcgg caacgtcgcg
    19741 gagaatgccg gcgggcccca cacgctggct tacggtgtca ccaccaacca cgtcctgggc
    19801 ctcgaggcgg tgctatccga cggtactgta atcaccaccg gcggcaagga gctggatctg
    19861 ccgggttacg acctcaccgg gctgttgaca ggctccgaag gcaccatggc gctgatcacc
    19921 aaggtggttg tcaggttgat gcggcagccc gagatggtga aaaccgtttt ggccatctat
    19981 gacaccaccg aaggcgccgg aaatacggtc tcggagatca ccgcgcgggc gatcactccg
    20041 gtggcggtgg agatgcttga cggcgtcatg ctgcggatgg tggaggaggc gacccacgcc
    20101 ggatatccga tggatgcctc ggcggtgttg ctggtcgaac tggaggggct tcgagaggcg
    20161 gttgaggagc aggtggagca ggttcgcgaa gcatgtgaag cctgtggagc gagagagttt
    20221 cgtgttgccc ggtctgccga ggaacgcgac ctgctctgga agggccgcaa gaacgcgttc
    20281 ggcgccgtcg gccgcgtcag ccccttctac tacgtgcagg acggcgtggt gccgcgtacg
    20341 cagatcgctc cgacgctccg gttcatcggg caagtctccg aaaagtacgg gctgacgatc
    20401 agcaacatct ttcacgccgg cgacggcaac atgcacccta tcatcctgtt cgacgcgcgc
    20461 aagccaggcg atctcgaaaa ggcaagagcg gcgggcgaag agattttgct gcactgtatt
    20521 tcggtcggcg ggacgatcac cggcgagcac ggggtcggca tggaaaagaa cgagctgatg
    20581 ccgaagcttt tcagcgagga cacgctggaa acaatcggcc gcttcaagca gctcttcgac
    20641 ccgcagtgct tctttaatcc cggaaagctg ctgcccaccg gcaaaggctg cattgagatc
    20701 cgccagcccg cagtcacctc ggagaccatg ctatgaccac acgacgacag ttcctcttct
    20761 tgccggcggc gctggccgcc gctgctccca agctcggcca caaggagcgt gtcgaccgcg
    20821 cccttcgcgg agaggatgtg gaccggccgc cgtttagctt ctggtatcac ttcggcctcc
    20881 agaagttccc cgccgaaagg catgcgaatg caacgttgcg gttccaccgg aaattccgta
    20941 ccgacatcgt caaggtgatg agcgatttcc cgtacccgaa gccgaagggc aactggtacg
    21001 acccagtcgt ggtggacgat ccattccctg agcaaatccg cgctctcgaa atgatccgcg
    21061 aaggactcgg cggcaaggcc catttcgtcg agacgatttt caacccgtgg aacgtggccg
    21121 agaagctctc ttcgaaagaa gaggtccaga agttgaagtc cgagaagccg caggtgctgc
    21181 ttgatgtcct ggaggcgatc gccaagtcgg aggcgaatca cgcccggcgt gcaatcacga
    21241 ccggggcgtc ggggatcttt ctggcgattg ccaacgccgg cgacggcgtc ctggacgagt
    21301 ccgggtacgc gaagttcagc gagccgttcg acaggatggt gctggaggcg gcgagcggag
    21361 ccccgctgaa caccctgcac ttgcacgggg cgaagatcta cctcgaccgc ttctggaaag
    21421 gttggcaggc gccggtggtg aactactccg cccacggcac cggcgtcggg atcgcggcgg
    21481 cacgctcgaa atacgacggc gtgattatgg gcggcattga cgaacaaaac taccgcacgc
    21541 tcacgcccgc gcagttgaaa gagcagtggc gcgtggcgca acaagccgcc ggcaagaagt
    21601 tcttccttgc tcccggctgc tcagttccca atgaaaccac cgatcgggag ttgatgaatc
    21661 tcgtcagggt cctcggcgcc tgagactccg cgaaccgaat cgtctatgag acaacaagga
    21721 gccttttcca gaacactact cctgtcgctt tgcctggcac tctcagccgc gggcgttgcg
    21781 gcgggagccg aatatccgcc ggcggcgagc tacacctgct accgggcgga aggtccgatc
    21841 cgagtggatg gccggctcaa cgagtcgtcg tgggtcaagg ctccgcggtc gaatccgttc
    21901 gtggacattg ttaccggcag accggcttgg cacgacactc gggtggcgct gatctgggat
    21961 gaccgttatc tttacttcgg ctacacgatc gaagagccct acgtccaggc gacgctaacc
    22021 gagcgggatt cgaagattta catcgagaac gacgttgaga tgtttatcgc gggaaaagac
    22081 gcctactacg agctggaact gaatgctcgg aacaccgttt atgaagtgtt ctggatctgg
    22141 aaggacatct acaagccggg gtcgccgtac ttcggagttc cggagttcga cgtgaagtcc
    22201 cacacgatga tgaccctgtc cggcgtcggc ggacacatcc acccgcgtgg cgaacggtgg
    22261 ggttttctcg actgggatct gccgcgcctg aagaccgccg tatttgtgga cgggacactg
    22321 aacaacccaa aggaccgtga ccggggttgg actgtggagc ttgccgtgcc gtggcaggga
    22381 ctgaagtggc tggcggatgg gcgcgcgctg ccgccgaggg cgggcgacgt ctggcgggtt
    22441 gactgctcgc gttttcagaa gtttggcccg gacggcaagc cgatggatgg gagtgcgggt
    22501 tggacatgga accggcacgg gcactacgac tcccacatcc cggaaacctt cacgcgagtg
    22561 cgattttcgg ccgagaaagt cggccgggag tgacattgcc acaatattcc tcttgacaat
    22621 tgtcgttaca acaaatatag tgaaattgat gaccgagagg cacaccgagg aagaggcgtt
    22681 cgtcagcctt cagaagaccg cggatgccct catgcagggg atcgccgtat tgctcaagcc
    22741 ggcaggattg tcggcgacgc agtacaacgt actccggatt ctccgcggcg ccgggaaaga
    22801 tggtctggcg tgcggagaga tcggtgtgcg catgatcacc cgggatccgg acatcacgcg
    22861 gttgctggac cgtctggaaa cgcgaggtct ggtaacgagg gcgagagaac tgaaggatcg
    22921 gcgggttatc acggctcgaa tcacgaaaga cgggctgcga atgctgaagg agttggacgc
    22981 accgatccag cggtaccacg agagccaact gggacacatg ggtgagaaaa aactggtcga
    23041 gctgatttgg ctgctggaac tggcccggcg gccgtcgatc tgatctctaa gaaccgctgc
    23101 ttcacttccg accttgagtt tcagtcgacg cttctggcgg cagccgtcgc gccgatggcc
    23161 gccggaggcc gtaaacaagt accgacagaa aggggaatgg gatgattacg ctacgacaca
    23221 gagacgagag gggagtcagc cggctgggct ggctggacag ccggcacacc ttttcgttcg
    23281 accactacta cgacccgcgg cacatgggtt tccggagcct gcgggtgatc aacgacgacc
    23341 ggatcgcgcc gggcgccgga ttcccgacgc acggccaccg ggacatggag atcgtgagct
    23401 acgtgcttgc gggagcactc gaccataagg acagcatggg caccggatcc accatcgccg
    23461 cgggggacgt gcagcggatg agcgctggaa cgggtgtgat gcacagcgag ttcaaccatt
    23521 cgcaatcgga gcctggacac ttcctccaga tctggatcct gcctgagcgc aaggggctcg
    23581 tccccggtta cgagcagcgc accttctcca cccaggagaa gagagggcgg ctgcgcctgg
    23641 tcgcgtcgcg ggacggcagg gacggctcgg tgacgatcca ccaggacgcc gacctgtacg
    23701 cgaccgtgct cgaaccgggc gagggcgcaa cacacgcctt gcgtcagggc cgggccgcct
    23761 gggtgcaggt agctcgtgga aaggtggttg tgaacgggac agctctcgag gcgggtgatg
    23821 gcgcgtctgt gaccggcgag tccaccctcg agatcgaagg caaggaagca gccgaagtgc
    23881 tgctgttcga tctgggataa tcgactgtcg gctttcgctc cgggtcccga aatgaacgag
    23941 acgatcgatt ttctcccgcg gcacggatac gcggtccttt tcgtgttcgt gctcgcagag
    24001 caggtcgggc tgccggttcc cgctctaccc gtattgctgg cggtgggggc gctcgcggcc
    24061 tcggggcagt tcggattcgc tccggcgctg gcagtagcag tcgtcgcggc gaccgcggcc
    24121 gacttggtct ggtacgaact gggacgacgg cgtggacaca cggtggtgaa tctgatgtgt
    24181 cgtgtctcgc tcgagcccga cacctgcgtg cggcgtaccg aggacacttt tgcgcgctac
    24241 ggccttcgca cgctactctt tgcgaagttc attcctgggc tgaatacggt gacgccgccg
    24301 ctggcgggca tggtccacac gccgatgggc cggtttgtgc tccacgacgc agccggggcg
    24361 gcgatttggg cgggcgcatt catggggctc gggtatctgt tcagcaccga gctcgagcgt
    24421 gtagccgtac acgctatgca gcttggcggc gggctgttcg tgcttcttgc gggcggcctg
    24481 gcctgctaca tcgtctggaa gtacgttcaa cggaagcggc tgttctggcg tttgaggacg
    24541 tcacgcattg ctcccgagga actcaagcga cagatggaca agggcaaccc tccgataatc
    24601 gttgacctgc ggcattcggt cgatctcgat gtggacggtt ttcggattcc cggcgctctt
    24661 cacatgaaac ctgcagaact cgaacggagg cacggagaga ttccccgcgg ccgggagatc
    24721 gtcctgtact gcacttgacc gaacgaggcg acgagcgccg gtctggcgcg caaacttcac
    24781 cggttaggga tcaggaacgt tcgaccgctg gagggcggcc tggagggatg gctcgagcgc
    24841 gggtacccag tggagccatt ggaggtgccg ggggtatgag aaggaccgct aagattgtcg
    24901 ttctgcttgc ctttgccgtg gtggtctcag gggctcaggc gggcgaggcg aggtctcgga
    24961 ccgactggtt ccacgacgca cgctggggtg tcttcattca ctacctggcg aagagcgacg
    25021 acgtatccgt cgacgagtgg aaccgggtga tcgacggttt tgacgcggag gggctggctc
    25081 aacaactcga gtctgcgcgc gcgggttatc tcatcctcac gctcgggcag aactccggcc
    25141 actacctgtc accgaacgcg acctatgacg ccctcaccgg catccggccc agcaaatgct
    25201 cccggcggga cctcgttgac gatctttacc gcgtgctcgc cccgcgcggc atccgattga
    25261 tggtctacct gccgggaggt gcgccggacc gcgacaaggt cgcgatggag aaactcgagt
    25321 ggaagacggg gccgcatcga aacttggaat ttcagcggaa gtgggagcgt gtgatcgcgg
    25381 agtggtcgga gagatgggga cggaaggttt ctgggtggtg gtttgacggc tgctattggc
    25441 cgaacgcaat gtacaggtcg atgaacccgc ctaactttgc gagctttgcg ggcgccgcgc
    25501 gaaaagggaa tgccgactcg atcgtggcct ttaaccgcgg cgtgattttt ccgatcatct
    25561 cgatgaccga acaggaagac tacacagccg gcgagatgga cgacccgtcg aaagtgcagg
    25621 tgagcgaatt ctggctggac ggcgcacagt tccacatgct cagctacctc ggaaaagcgt
    25681 ggagcgccgg tccgccgagg ttcacgacgg accaggtggt agagttcaca tcgagaatta
    25741 cccgcaaggg tggcgtggtg tcctgggacg tcccgaccga cgcgcgtggt ttgatcctgg
    25801 agccgtttct ggagcagcta cgggcgatcg gggcggtggt tcgctgacga ggtctaccgg
    25861 taaaccggct ggacggcgag gccttcctgc agcggtgtat ctgacggcag ggcgacggcg
    25921 tctccttcgc cgagaccatc gagcacctgc gcccgcgtaa tgctggaggg gccgagcgtt
    25981 acgttgcgcc aggcgatctt gtcgccctga agaacgaaga cgccggcctg ctgcgagcgt
    26041 cgccggagcg cctcgcgcgg tactgtgacg gcgttctcga cgacacgcga gcgaatctcg
    26101 gcgttgatgt tcgtgcccgg caacaaatcg cggtttgggt tctcgataat gcagatcact
    26161 tctccgacct gccgtgtacc ctgtgcaacg atcccggtgg gcatgcgttc gacagccccg
    26221 gtccatttcc ggccaggcag tgcgtcccat gtgatggtga ccgggagacc ttgggctacc
    26281 cggccgagct ccggctcgtc cacgtaaacg cgcacccgaa ccttgtcgat ctgaccgacc
    26341 ttggccacgg ggtcaccggg gctcaggtag gaaccgaggc gaacatcctt ctcgtatacg
    26401 acgccgccga tcggagatcg gatcgacgca cttgcgatcc ggctgcgcgc gagttcaacc
    26461 gatgcctcag cttcccaaag ccgggcctgg gcggcggtgc ggtcggtgtc ggagacgagc
    26521 gcggctcgtt tccgctcaag cgaccggata tcagcctgcg cgcgttcaag ccgctgggtg
    26581 gcctggtcga gatcctggcg ggtcgctgcc tgcttctcga caaggcgccg cagggattca
    26641 acgtctcttc cggcgatttc cgaatccgaa cgggcacggc gtaaggcgct ctcgatctcg
    26701 gccagttcag tggaacgccc gccgcgctga agcacttcga gttcggcccg ggcctgcatg
    26761 acgcgggctt ccgcggctgt cagttcggtg cgggcggtgt tcgcgtcgag ttgggccagg
    26821 actgcgccac gtgctaccgg ctgtccgact tctaccagaa cccggtctac gatccctccg
    26881 tgttcggcgc gaatctcgac ccactccagc ggttctacct ttccgttggt gaccagggtg
    26941 ctgaccagcg tctcgcgctt caccttggta aacgggacct cgggcgcggc ggaactccgc
    27001 ctgtaaaccc accaaacgac cgccagcacc ggcaccagca ggacgagaag gagccttttc
    27061 atatcagcga cagtaaagtt gccgggcgat caccggtgca gcatcacgcc atgaccgatt
    27121 tgcatcgctc gtctcattgt gacaaatgaa agagaggcat gggtgcgaac agcaagacgg
    27181 tggtggtctt ttcggcgggc atgatgtttg gggcatacca ggcgggtgcc tggtcggcgc
    27241 ttgccaagga gttgcgcgtc gacggtgttg ttggggcgtc gtccggcgca ctgaatgcat
    27301 gggttgtcgc cggcggctgc gacccggaca cgctgcggga gatgtggctg agtaacgaga
    27361 ttgcgtccgt caccaggctt cgcgcgccgc tgccgccgtg gagcgggatc ttcgactcca
    27421 agcgcctcga gcgtctggtc cgcggaatct acgattcatt ccgaccgcgg atggagatcg
    27481 gcatcgtggc gacagaggtc aggaatttcc gccggcgcct gttccgcgaa gaggagatca
    27541 cctggaggca cctggccgcg tcctgcgcca tcccgtttgg atttcagccc gtgcggatcg
    27601 atgaccactt ttataccgac ggcgggatgc tcggagccct gcccctttgg gcagcgtctg
    27661 aaatgggtgc gacgcggatc gtggcggtac tggcgatgcc gagacttcca tttgccctcg
    27721 gccgtgcggt tctcaaggtc gtgcgggcag cggctccgga gacgtctcgg gttccactcg
    27781 gcttgcagat tgaggtaatc cagcctagcg agccgatggg atctctgcgg gacctgatcc
    27841 tctggcggcg ggaaaacgcg gagcggtgga tggacctggg gttccgggac ggggccgagg
    27901 cgctccgaag gcttgtgtga gagaatacaa gccgaatatc cgaagaggag gtccgcactc
    27961 atgaacccta acaatggtgg ttgctgtgct tcgacgtgta cgacgtgccc cgtcagccgc
    28021 cgcggttttc tcaccgccgg aatggcggca tcggttcttc ccgccgtctc cgccagttcc
    28081 atgaaagacc cggcgcgcca gcagccgatc cgcatggccc tcaaggtcca gcccgtgctc
    28141 gtgtacgacc tgtacaagcg gagggaggcc acgagctggc ggccgtgggg cggattaatg
    28201 aacgagcaag acgtcgccgc ggagaaagaa cggatcggca aggaactctc gtcgatgaag
    28261 acgtcggcgg actttccact ggaggtcctg cctctggcga ccgcgagaaa ccaggatgag
    28321 gctgcggcga tcgcgagggg cgactccgac gtcatcatct cctatgccgc cagcggggac
    28381 gggcggatct tcgagacgct gaccgttcca ggcaagtggc atctgatgtt cctgcggcac
    28441 cgctccggcc cggtctactt gtggtacgag atcgcgcacc cgcggttctt acggaaaacc
    28501 gtggacgagt tcggacagcc cggcatgaac gtgcatgacg tcgtggtgga cagccacgcc
    28561 gagatcctga ttcgactgcg agcgctgggc gccctgaaga acacgatggg caagcggatc
    28621 gtggcggtcg gcgggccgag tggttggggc gccggaggca ggaatgcgcc agatctcgcc
    28681 agggagcagt tcaagttcga ccttcagacc gtgccgtatc cggagctcgg cgaacggatc
    28741 aagcgggcgc gggcggattc ggcgctggtg aaacggtgtc agggcgaggc ggagatgtat
    28801 ctcagggaca aggcagtacg gcttgaaacc tcgcgggagt ttgtccagaa ctcgttcgtg
    28861 ctgacggaag tgctccgcga tctgctggat gaggcgaaga cggatgcgct gacgatcaac
    28921 cagtgcatgg gcaccatcat gccgatgtcg gagacgacgg cgtgcctgcc gttgagcctg
    28981 ctgaacgacg acgggtacat ggcattctgc gagtccgact tcgtggttat cccgtccggt
    29041 gtgctgctgc attacatctc cggcaagccg gtgttcctga acgacccgac gcatccgcac
    29101 gacggcgtgg ttacaattgc ccactgtact gctccccgga agatggacgg gatgcatgcc
    29161 gagcctgtgc gcgtcctgac gcacttcgag tcggactacg gagcgtctcc gaaggtcgag
    29221 atgcggaagg ggacgaagat gaccaacctc gtgccggatt tcgccagtcg caagtggatc
    29281 ggtttcgaag gcgaggtgat cgacaaccca tttatggcga tctgccgctc ccaggtggac
    29341 attacgattg aagggagcac ggaaaaactg ctcgagcaga tgggcggatt ccattggatg
    29401 agctcttatg gtgcgtacgt gaaagagacc aagtacgcgt tgcggaagat cggcgtggag
    29461 cttgtgcaat taacgtaacc ggcgcgacca gcgagaagaa catttacagc ggggaatgtt
    29521 ttgaccgata atattggcct atgcccgtgt accggatcca ccggttgaag gaaaaccaac
    29581 ggcagcagtt ccgatgggcg ccgcatacga ccggagccac tgacgtcaaa ccgaaagatt
    29641 acgagcaggc gggatcagtt gaggccacta attcgtattc ggcgtggttc gcgcttaagg
    29701 acggtgagga tgcgctgaag gtcggcgacc tgctcgagtc ggagagcggc gatctcctga
    29761 tctacaagta cgtgggcttc gaggcggccc gatgggtgct gccggaggtg aagccgggaa
    29821 tcgagagcac tccgccagcg gtggagaaca gtcggaacga gggcgcggcg gccttttcag
    29881 ggtaagatag ggaaatgcag tctgataccg accctggcgg ccggatcaac ctcttctctt
    29941 tggtggccta catccccgat ccgcttggcg gctacatgga ccagctccgc caggaacttg
    30001 taccatcttg ccgcctgagg gcgcacgtca ccgtgctgcc gccccgcccg atctccgaag
    30061 aagccggcaa agccatcgag cagttacgaa gccagttgcg agacctcgtg ccgttcgacg
    30121 tacaggcgaa agacgtcgac atgttctcca tcacttccgt ggtgtatgtt gcggtggggg
    30181 ccggcttcaa taagctcagg cgcatgcacg acgtgctgaa ctccggcgcc ctgaagttct
    30241 gcgagcccta tccttaccac ccgcacctca cgttggcgca acaaatgacg cccgaacaga
    30301 tcggcggagt ctacgaaacg gcgcgtatcc gttgggcgca atgtcctttt gagcgcgtat
    30361 ttcccgtgga tgttctgacg ttcgttcagg ggactacgat gggaacctgg attgaccttg
    30421 ccgagtgccc gatcgggcag ccggtggcga cccccgggcg gtcgtagggt gatcagctac
    30481 tcaaacatct ccatctgatc ggttggggcg cctttcttcc gccgcctttg gatggggcta
    30541 ccgacaactc caacgacatg aaattctcgt aatgccgcct tgggcacgaa gattcgctgg
    30601 ttgttggaca cggggttcgg aggcacgaca gtgaagccaa aagcctccaa ctgtaggaga
    30661 ttgttgggca aaagaccatc catcaactcg ccgtcgttga aacacatccg gacccacaaa
    30721 ccttcggttt tcggcctcgt gtggaaaagt cctgggctgg cgcttcctcc cggagcctcg
    30781 aaatctctaa caaaacaaac ggctttgatc tcaccgtagg ggaccaatag gacgctgccc
    30841 gtagggctca gcaactcgat gccttcgggc tggagatagt cgtgcgggtt cacgaagcct
    30901 gtcacgggct cccggtcgaa ccgccggatt accactttct tgctggttga cgccgccaag
    30961 cacttccctt tctcgaagct ttgctaccaa acatttccgg aggtattgta tcattggcac
    31021 aatgcgggag acgtccttaa tcggaacaca agttcgggcg ttcctgaatt cctgccgcgt
    31081 tgagaagggg ctggccgaaa acacggtaga agcttaccga agggatcttg ggaagttctt
    31141 atctttcata ggcgtcgagg cggctggcga aatcccccgg gccgacgaac tcgggcgata
    31201 cctcgattcg ttgtatcgct ccgggctggc aagtcgatcc atcgcgagac acctgactac
    31261 gctgagaaat ttctacggct ttttagcgcg ggaagggcag atcgagtccg acccgacgag
    31321 tttcctgacg agtcccaggc agtggaagaa tctcccgaag tacttgaatt tagaggagat
    31381 taatcggttg acgatggcgc ctgatccggc cagacccacg ggtgtgcggg accgcgcgat
    31441 gttggagttg ctgtacgcaa ccggattgcg ggtctcggaa ttgtgcaact tggagctttc
    31501 cgacctggac ccggcacttg gcatcctgag agtgacgggc aagggtggga aacagcggat
    31561 cgttccagtc ggcaaagcgg cagttgcagc gattgaagcc tacgtggcga gcgcacgtcc
    31621 gcaattgctg cgaagccgag gaagtcgtta cctttttgta acggctcgtg gcggccggct
    31681 tacgcggcag gccttctgga agctcctggc tgtgcatgga aagcgggtgg ggatattccg
    31741 caggctgact ccacacgtcg tgcggcacag ttttgccacc catctgctgg aagggggagc
    31801 ggacctccga agcgtccaga tgatgctcgg acacgcggac atctccacga cccagatata
    31861 cacccatgtc ctacgctcga ggttaagaaa aacagtggac cagcaccatc ctcgacgata
    31921 gcggcccctc ggtggcccga aaggactcat ccgagagcaa atgcaatgag cgactacatg
    31981 ttcatgctgg agaaccacct gagtgcgccg cagaaccggg tggtgaaaga ggtccaggcg
    32041 gcggcggagc acggcaacgt gaacctgttc ttgacaggcg gtgctgtccg ggacatgctg
    32101 ggcggttttc ccatccggga ccttgacttc accgtcgagg gtaacgccct gaaagtggcg
    32161 aagttggcgg cggagcgggc gggagcgacg ctgctgaagg ccgacgaaga gcggaggaca
    32221 gccgatctca tctttcctgg cagcgttacg gtggagatcg gtatggcccg gagcgagcgg
    32281 tattcaaagc cgggtaaacg ctcccacgtt tcccaatcga cgattgtgga agacctgcgt
    32341 gggcgggact tcacgatcga ctcgattgct ctgtcgctga accgggcttc gctcggcttg
    32401 ctcctcgatc cgaataatgg ccaagccgat ctggagcacc gggaactccg ggcggtacac
    32461 aaccacattt ttttcgaaga tcccgggcgg ctcctgcggc tagtgcgttt ccgcgtccgt
    32521 tttggaatgg tggtggatga caggacccag caacaatacg aaaacgctcg attggaggct
    32581 ctggaacggc tgattccgcc gcgggggcta tttaaggaac tgcgggagat tgccgacgag
    32641 acgagcccgg gtgaggttct ggcggcgctc gagacggaga aactgctgcc tttgttctcc
    32701 gaggcactcg ccggcccgaa gctgaacacg gcaggactgg cgaagtggca aaaggcaaaa
    32761 caggcgattc cgttcggtgt ggagattggg gcaaacaacc ttgcgcttct cctgtatctg
    32821 ctgacggaga aactgacgcc gaaggaacga gccgcactgg tgaagtcagt gtccatgcac
    32881 aagtcggaga tcgacctgtg gcagacgctg gaggccaaaa cgcgaaagct ggagagatcc
    32941 gtcggttcgg cgaagattca taagccgtca cacatctacc gaatcctttc aagcgctgcg
    33001 ggcgagcaga tcctgttcct gctcatacgg tcgcagatcc ggctcgtgca cgaccgaatc
    33061 aagaactact tgcagaagta cctaccggct gcgcaggaga tcacggacgc ccaagtggcg
    33121 gcggcgggtc tccagcccgg cacaacgaag ttcaggaaac agaaagagga gatgattgtc
    33181 gcacgactgg acggacgcgt ccggaaaccg gctgccgccg aggagcctga agagcatgcg
    33241 gccgccgttc cagcatcggc ggagaggaca ccatatggga ccaatcgatc ggcgaagatt
    33301 tctcctgggc gcaagcctgg ctctgccggt gcgcggcgct gagactaaga tacagatcgg
    33361 gaccgtcgga gttcgaggcc gcggccggga tcacattgag gagatcgcgc ggcggcctga
    33421 cagtctcgtg gcggcggtct gcgacgtcga caccgcgcag accgagcggg cggtggcgct
    33481 ggtagagaag ctccagggca gcaagccaaa ggtttaccag gactaccgga agatgttgga
    33541 tgaccaagac gtccaggctg tctctatcgc cacatgcaac cactggcatg cgctgcacgc
    33601 gatctgggcc tgtcaggcag gtaaagatgt ctatgtcgag aagccggcga gccacaacat
    33661 ctgggaaggg cgccggatgg ttgaggcggc gcggaaatac agccgcgtca tgcaagtggg
    33721 catgcaaagc cggagcatcc agcataaacg cagggcgatg gacctgcttc gacagggagt
    33781 gatcggcaag atctacatgg cgaagggcat ctgttacaag cgtcgccctt cgatcgggaa
    33841 gaagccggac ggcccggtac caccgggggt caattacgac ctgtggctcg ggccggcgcc
    33901 gatgcggccg tttaacgaaa accgttttca ttacaactgg cactggtttt gggacacggg
    33961 taacggcgac atcggaaacc agggcgtcca cgagatggac atcgcgcgat ggggtctgaa
    34021 caagagtacg ttgccgaagg tcgtacattc gagcggcggg aagttcgtct atgacgatga
    34081 ccaggagacg ccgaacacgc aactgtcggt tttcgagtgg gacgattgtc agctccattt
    34141 cgaggtccgc ggcctgctga cgggcggcga gagcagcatc gcctacgacg ggcggaattt
    34201 catcggcaac atgttcttcg gcagcgacgg ttacatgtcg gtcgatcagc gtgggttcca
    34261 ggtgtaccgt ggagacggca gagaacttac cgaacggatg gactacgccg agccgcgcat
    34321 ctgggacacg gcgccgcaca tcggcaactt catcgaggcg gtaaagagca ggaatgcaac
    34381 cgatttgcac gctgacatcc tcgagggcca cctgtccgct tctttgtgcc acatggcgaa
    34441 catcagctac cggacgggcc ggaagctcac atttgatcct gtctcagaga cgttcgtgaa
    34501 tgatcccgag gcaaatggtc tggtgaagag gaagtaccgc gagccgtacg tagtgccggc
    34561 gcaggtgtaa cgcctgccgc tagcggttgc ccaggttcag aaaggcgatc ccgagaagga
    34621 tcaggccgat cccgacgaac ttattccggg tgaacggctc gccgaagaac agacgcgacc
    34681 aaaagagggt ccagatatag ccaagcgaga ccatcggata gaggatcgtc agttcgccac
    34741 gccggatccc caacacgaaa aagaacgacg acagcaggaa catagcgacg ccgagcgcca
    34801 gccgccagtt cagaatgagc gtccgaatct ggcggtggag ccggccggcg ccggatttca
    34861 aaaaaaccgc gccaaaggac cctatgaacg atgccagcag caccagcagc atcgaagaga
    34921 cgggtgtttt cattgtttgc cgttcttccc gaggaccgcc acccctgtca caatgatcag
    34981 cactcccacc acccggaacg ggtttaccgc ctcatggaag aagaccactg aaaggacagc
    35041 gacccacacg tacgtcagag agatgaccgg gtagagaatc gataattcgc catcgcgaag
    35101 cgcaagcaca aggagcagtg tgacgatccc gtaaaggctg tacccggcca taaggggcag
    35161 gttggtgatc attgccagcg ggctcgaact ctggagcgta ttggcccccg acttaatcag
    35221 gatctgggcg gcagccgcga acaacgtaca aaaaaagacg aggacaattg actgtttccg
    35281 gttgaactgg aaggactttt ttgcgggcgg atgtgtcgaa gcggtgttca atcgatctcc
    35341 ggacgattcc atatcatacc cgatgcggcg aatcggcggg gggctcgcgg gagcagctcc
    35401 ccggacgccc tcaattccct cagcgtttcc tcgacggtgg ctttgctggc gccgtagccg
    35461 gaccagcggg gttcccctcg gagggtttcg gcgattcgac catgggttcg ggaaggatct
    35521 gttccttcgg tttgcattgg cccagctcga tcgagaaagc agccaccgtc tgccaggact
    35581 ttccctggcg cttttcgatg cggtaggacc cggaggccgc gtgagcgcgg acccgccaac
    35641 cagtgtctct cggtgctgcc gccactccct tgaccgggag gggcttcgcg ccggcttccc
    35701 gtatactcca gctttcgcca ccggtcatcg tctcgtatcg ctcgtgccgg gagtcgatct
    35761 ctccggattg ggtgcgatcc aaccagaggg acccttcggt ccgcgaatcg aaccagaact
    35821 tttcgatggc gccgattcga gattcctcga aaagtggttg ctgccgccag gtcttcccac
    35881 cgtcgctggt caacagcagg aacggatcgc gcggcaaggg acgcacaagc tgacccccca
    35941 cccaaccggt ctcgaagtcc aggaactgga tctgttcaag aatagccagg cggattcgct
    36001 catgaggttc gctccacgtt ttgccgctgt catcgcttgc cagcagcacc gaactcaacg
    36061 tcgaagaagc cgtgtggagg ttgccggtaa caaatagccg ggccccgacg ggctcgacgg
    36121 cagcgagctc caaatagacc gtgcagggcg aatccgcggt acaggccagg ccggcggact
    36181 ggatttcttc gtcgccgcac tcgagtggaa cggagatggg cgccccggtg ttctggagta
    36241 cgccgttcgg tgaacctggg gctggcagct gctcctgccc gcaggccgcc gcggcgagga
    36301 gaaaggcaga gaacaagatg agcgggatgc gcatcgttag tcccttttga ttgtagccgc
    36361 acgcgaccca aggccgtgaa atcatgccga ccgatcaacc aaagttagta ttgcctgtaa
    36421 gctaaaggaa acattgacag aaagtgacag cggctttaca ctcttcgtga acgctggctc
    36481 accagtcagc gggggagaag ggcgctcatg atcagtttgt tcgacacgaa acaagaggac
    36541 gaatcccaga agacccgaag agtttatatg gatcgcctcc atgagattgc gtacggttca
    36601 gccggagcaa aggaggcgga ggcggcaaca accttggcgg atccccccgg agaggctcca
    36661 ccacccgaaa ccagtgtcaa ggaagccgaa tctatccccc aacccgaagc agtctcagag
    36721 accttgggaa catcggcctt gcccgacagt gaaccgttct ctttccaggg aaagatgaaa
    36781 gtggaggcgc tcacggaggc aacaacagtg gattcgcgga aacttgcaga ggagattgct
    36841 ccgaaattcg tcgacgtgtt cgtcgccacg tttcaggaga tggggcgtca cgcctcggct
    36901 tcaaacggcg cggtatccga ggtcgttgcg gaaatgaacc gggtgtcgca gcatttgcag
    36961 gtactcagcg gacaaatggg cgagctccgg cggtcgatcg attcgatgac ggcttcccag
    37021 caagagcttt ccacgcgagt gtcggacatc gaagccggcc tgcgggagca cgagaaggtc
    37081 tacctgaacc tcgaggccgc catgacgcag tccgaacagg ctctccgggc acagcaggac
    37141 ctcctcacgg agaaattgga ttccgcctcc gcacaggtag ctcagatcaa cgaacggctc
    37201 gattcgcagg caggcgcgat ccgcgcgctc catgagtcgt cccggaaccg cgaagagcat
    37261 cgcgacgagt tgcggtccgc gctccaggcg atccaggacc tcgcaagcaa actatctacg
    37321 ccggagccgc tgccggacaa gctttaggat cgactgcagc cccggcaact ttgctcacat
    37381 catcccgcaa gtaccacaac ggcattctgt ccgctctcga gcagaccccc atgttagaat
    37441 cccatcgtag cggcacacgc acggggcggg tttccgaatg tttgggtggc tcaagaatcg
    37501 acgcgaaacg cctctctccg gagcgccggc ggtaaggcgg cagaagagtt actcggcgca
    37561 gagcggcttt gtttaccagt atttctacca ggggaatcgc tcggcgatcc gggagcggga
    37621 acagggacag gaattcgtat ttgaagtcac tgtggaccgc aagacttcgt ttccggtatc
    37681 cgtgtttgtg agcgaatccg tcgtggtaga ttggcagaac ctgcacaacc gcgctctcag
    37741 ccccaccgaa cgctatgcca tcgcgaagat ggctctgttt caagcttttg atgaccgccg
    37801 gacgccgggg gagatgcgag aagaagtccg ggtccggaca gccgacgtgc gggggatcct
    37861 tgacatactg ggaatcgagt gaccaaaccg tgaaggcatg acggagctcc tgctcacgac
    37921 ggctgacggg cacgcgcgca gcgtgccgct ggagggcgac ctcgtgtcga tgggccgttc
    37981 tcccgaaaac cacctcgctt ttccggaaga cggcttgctg tctcgccgcc acctcgtgtt
    38041 caaaccggcg acggatggat gggcggtcca ggacctcgac agcaagaacg gcaccttcgt
    38101 gaacggccgc cggattgagg gcgaaagaac tctgcgggca ggcgaccgca tctccgccgg
    38161 cggtctgaaa ttcgttttca agggaaccgg cgagctaacg ggcgaagtca cgttcgaggt
    38221 tcctccagga gaggattccg tgctgcggac gaagagcatc tcgctcccgg aggcgctatc
    38281 tgtcgtatcc tcgcggcgcg ggcgcgtcag tagcgggaca cggaaggcgt ggcggttcat
    38341 cgacggagtc cgggagttga acgtcccaag gccgcttgcc gaactccttc aggcgacgct
    38401 taacctggct atggaagcgg cggaagccga gcgaggtgtg ttgctgacat tcgacgaaaa
    38461 ggaccggttg acagcccagg cttccagcgg cggcgacttc cgaatcagcg ccactgtgcg
    38521 cgaccgcgtg ctccgggacc gggtttccct gctggtcgag gacgtggcaa aagacgagcg
    38581 gctcctgacg agtgacgtga tcgcacagag ccatatccgg tctctggtag cggtgccgct
    38641 ccagacggaa gaccgggtgc aagggatgat ctaccttgac accagccacc ccacccggcg
    38701 gttcaacgat gacgacctga gccttctcac agtgatggcc aatgtcgcca gcctgcggat
    38761 cgagcgggag cgatgggacc tccagcgccg ggcgctgatc tcggagcaca tcaccacgct
    38821 tcagaagctc gcggcggcgc tcagccacga actgaactcc ccgttgggaa cgatcaagag
    38881 caccatcgat acgcttgtac gcgtgtcagc acgtgaggaa gcaggtcccg gagcccggga
    38941 atccgatctg gcctcgctcc gggagaatct gcacagtacc ctggaggctt cgatcagcag
    39001 gatccaacaa gtgattgcgc ggatccagcg gttcacggac ctggatcggg ccgagatcca
    39061 atccatcgac ctgaacgaac tgctgagcga cacgctggcc caactcgacg ttccagagga
    39121 tatcgatgtc gagttctact tcgacgctct gccgccggtg gtctgccggc cgcgaaacct
    39181 gaacatcgtg ttcttgagcc tgctgcggaa cgcggtggac gcctgccggc agctccccct
    39241 gaagcgcggg agaatcctgg tttccacgca cgcgcgggag ggccgcgtcg aagtgagaat
    39301 tgaagacaac ggatgcggca tccgcggggt cgaccgtgag cagatcttcg acccggcttt
    39361 tcgtgttgtg gaaggccggg tagccgcggg caactggggg ttgttcagcg cccggcagat
    39421 aatccgcgaa cagggcgggg agatagccat tgacagcgag gagggtagag gcaccaccct
    39481 cctggtgaca cttccgggac gcgtcgtcga gcggcggatg ggaccggaaa gggactagcc
    39541 tgttcccgcc atctcctgcg catctaccgc cgcgatggcc gcgacattga cgatgtcgct
    39601 cacctcacag cctcgctgga gcacgtggac cggtctggac aatcccatta ggatcgggcc
    39661 gattgcctca cagccgccga ttctcatcag cagcttgtag gcgatgttgc cggattccag
    39721 gttcgggaac acgagtacgt tcgcgccgcc cttcagggag ctgaacgggt acgtctgatc
    39781 gatgatctcg ggggtcacgg ctgtgtcggc ctgcatctcg ccgtcgacca ccagcgcggg
    39841 aacgcgctgt ttgactagct gcacagcacg ctccaccttc tgggcgagag gatggcgggt
    39901 actgccgaag ttggagaatg agagcatcgc cacccgaggc tcgacgttga aacggcgcgc
    39961 tgtttcggca gccagaatgg cgatttccgc gagatcctcg tccgtaggct cgatgttcac
    40021 ggtggcgtcc gcgaggaaat acacatcgcc cttcagggtg atcaacatgt acagcccggc
    40081 aaccctgcgg agacctttgc gtacggaaat gacctgcagg gcgggccgga tggtgtcggg
    40141 atagtgttgt gtgaggccgc tgaccagggc gtccgcgtca cccattcgca ccatcatcga
    40201 cccgaagatg ttccggttca ggatcaattc ctcggcctca ctgcgcgtga tgcccttgcg
    40261 gtgccgccca cggtagagtt cttcgacgta tcgcggcagc gcctgacagt tcgagggatc
    40321 gactacctgg atcccgttca agtgaagatg caggtcgtcg atcctggctc ggatccgctc
    40381 ttcgctgccg agcagaatgg gcgcggcgat tcgctcgtcg agcaggatct ggcaggcgcg
    40441 cagaatcttg ctctcttcgc cttccgggaa gacaaccctc tgcggcttcc gctgcgcctt
    40501 gtggatcatg gagcgcatca gttcctgggc ttttccgagg cggcgctcca actgatcctt
    40561 gtattcgtcg atatctatgg gcgcctgggc cacgccgctt tccatggcgg ctttagccac
    40621 tgccgaggcc acccagatga gcaccctgtg gtcgaagggt ttcgggatga tgtactcgcg
    40681 gccgaactcc atacgctcga cgccgtaggc gcggcgcacc gagtcgggca cgtcttcctt
    40741 tgcaagggcg gcgagggacc gcgtcgcggc gagtttcatg tcgtcgttga tcgccgtggc
    40801 gcgaacgtcc agggcccctc ggaagatgaa cggaaaaccg aggacgttgt tcacctggtt
    40861 cggaaaatcg gaccggcccg tcgccatgat tacgtcgccg cgcgcggcca cggccacgtc
    40921 gtacggaatc tcggggtccg ggttcgccat ggcgaagacg acggggtcat cggccatcga
    40981 ctgcagcatc tctggcgtca cgcagcccgg tcctgaaagg ccgacgaaga cgtcggcgcc
    41041 gcggagggcg tcggccagcg tcctggcctc ggtttcccgc gcaaagcgct ccttatactt
    41101 gttcatcccc tccttgcgac ccttgtagac gacgcccttg gtgtcgcaca tcgtaatgtt
    41161 ctcgcgcttc accccgaggc ggacataatg ctcggcacag gagataccgc tggcgccagc
    41221 gccgttaaag accaccttca cctcgtctaa tcgtttgccg gcgacttcca cagcattgac
    41281 catcgcagcg cccgagatga tcgctgttcc gtgctgatcg tcgtggaaga ccgggatctt
    41341 catcgtgcgc ttgagttcct cctcgatctc gaaacactcg ggagccttga tgtcttcgag
    41401 gtttatgccg ccgaacgtgg gttccagcaa ctggaccgca cggataatct ccttcgggtc
    41461 ctcggtggcg agctcgatgt caaagacgtc aatgtcggcg aagcgcttaa agaggaccgc
    41521 cttgccttcc atgaccggct ttcccgcgcc agcgccaata ttgcccagcc ccaggaccgc
    41581 cgtaccgttg gtcacgacag ctaccaggtt ccccttgccg gtgtacttgt agatcaagga
    41641 cgggtcccgc tcgatctcga gacagggagt cgccaccccg ggtgtgtaag caaggctcag
    41701 gtcccgctgg gtgcggcagg gcttggtcgg cacgattgcg atctttcccg gcatgggcga
    41761 cgagtgatac tcgagcgcat cctctttacg aatagccata ggtaggattc ctttccacat
    41821 acagttgtac tccctttttt cagtctgagg ctaggcgatt gttcacccgg aagtgggtgt
    41881 cccggcagaa cacggcgcag gggtaagatg gaagccgagc gcatgtctgt ccctcccatc
    41941 ccgcctgagt tcgatcatct gagccacagg ccgttttcct tttacccacc gatcctgaat
    42001 atcgaacaca acgagtggct gctgcgccgc ggcacgtggt cggagattct ggtggtcaac
    42061 cgcaagacgg atcaggaggt atggataccc aggcgctaca tcggcgaagt ctcgcgtgtg
    42121 gacgagccgg tgatgatcgt tggcctcacc aaggagttgg aatacaaggg cggaacggtg
    42181 tggccgcacg aacggcgcgt gattgagatg ccccgcgcgg tgaacgaggg agtgtcgcgg
    42241 cagccggcca agattccgga ggaaccggcg ccagtggtcg gcatcaggct cgagccctcc
    42301 gaatcgcgca tcagccgcct cgtaggcgcc atgctgctcg ccggcgtgct cgtctgtgta
    42361 ctcgttgtcg tcctctcaca gcgatcgacc agtttccgtg caatcgagca gttcgatctc
    42421 ggactaacgg cggatgacga ttaccattcg atcgtccgca agctcggtac tcccagtgag
    42481 gaccggtggc gatcagaaac gggcgagctg caataccggc tgctacggtt tccggctcgg
    42541 ccctacacgc tgattctcat ggggacagat cgcgaccacg ctcactacgt cggcgcgctg
    42601 gacgcggaat ggagacccgt gaattcagtc cgccttccaa gcggaaacga cacgatggcc
    42661 atgctgaaga aactctcgaa gtggtaggac cggcgactgc tggagccgaa gcgctaaacc
    42721 aaagggacga ctaatgaagg ttgcaggata catcggttgg ggaatcatcg ccatcgtggc
    42781 ggcgtatttt cttggcggga tcgcgctcga gcgcggcgag ccagtaaata gcatgtggct
    42841 tgtgcttgcc gctggctgca ctttcctgat cggttaccgt ttctatggaa agtttatcgc
    42901 tgccaaggtg atggcgctca acgacgaacg ggcaaccccg gccgagcggc tgcggaacgg
    42961 acacgacttc gaaccgacca acaaatggat cctcttcgga catcacttcg ccgcaatcgc
    43021 cgggccgggg ccgctggttg gcccgacctt agccgcacag tttggttatc tccctgggac
    43081 gctttggatc attctcggcg cagtgctcgg cggggctgtg caggacttcg tgatcctgtt
    43141 ctgctcgatg cgccgggacg gcaagtcgct cgggcagatg gctcgcgagg agatcggcaa
    43201 ggtcggcgga atcaccgcgt tgctgactgt actgctcatc atgatcatcc tgctggcggt
    43261 ggtggcgctg gtggtggtga acgctctcaa aggtagcccc tgggggacgt tcacaatcgc
    43321 ggcgacgatg ccgatcgccg ttttcatggg gctgtacctt cgttattggc gtccgggcaa
    43381 ggttctggaa gtctccgcaa tcggcttcgc gctggtcgtt gcgagcattt tcgccggaca
    43441 atgggtcgcg cattcttcaa cggtcgcgtc gtggttcacg atgggcggca tagcgctggc
    43501 ggcggcaatc atcatctatg ggttccttgc gagtgcgctg ccggtgtggc tgttgcttgc
    43561 tccacgtgac tacctgagta cgttcgtgaa gctgggtgtg gtgctgatgc tcggtgccgg
    43621 catccttttt gtgcggcccg aactcgaact accggcgttt acccgctttg tggacggcga
    43681 cgggccgatt ttcgccggca agatattccc cttctgtttc atcaccatcg cgtgtggtgc
    43741 gatctccggc tttcattcgc tgatctcttc gggcacaaca ccgaagatga tcgccaagga
    43801 gtcgcacgct tggccggtag ggtacggttc gatgctgctc gaaggcttcg tcgcgatcat
    43861 ggcgatgatt gcggcctgcg ttctgcagcc cggcgtcttc ttcgcggtga actctccggc
    43921 ggggatcgtc ggggcaacac cggaggccgc caccgcaacg attacggcgt ggggcttccc
    43981 ggtcactgct gcggagatgg aggtcctcgc gcgcgacgtc ggcgagcgga cgctgttcca
    44041 ccggaccggc ggcgcgccgt ctctggccct cgggatggcg cacattttcg cgacgggcgc
    44101 gggcggcaag gcgatcctcg gcttctggta tcacttcgcc atcatgttcg aagcgctctt
    44161 catcctgacg atcattgacg cggggacgcg ggtgggccga ttcatgttgc aggacctgct
    44221 cgggcacgtc tacaagccgc tgggccgcac gagctggatg ccgggcgtga tcggaacgag
    44281 cgcggtggtc gttctcgcat gggggtactt cctcgttcag ggtgtgcgcg atccgttcgg
    44341 tggcattaac tctttgtggc cgctgtttgg catctcgaac cagttgctgg ccgcggtcgc
    44401 gctttgcgtg gcaacgactg tcctcctgaa gatgcatcgc gcacgataca tgtggatcac
    44461 ctgtgttccc ctggcctggc tggtggtggt gacgttcacc gccgggtggc agaagatctt
    44521 ttctccgttg ccgcgcgtcg gttttctggc ggaggcggcc cagcttcagg cggcgctcga
    44581 tgccggggcc gtggccgccg caaaagtgac ggagacgaat actcttatct ttaacgcgcg
    44641 tctcgatgcg gtggtctgcg gaatcttcct ggtgctggtg agcttgattc tcatcgattc
    44701 ggtccgtctg tgggtgggga tactgcgagg cacacgagag gcgaggataa cggagtcccc
    44761 gtttgtagtt tcacgtctgc gtccggagga gatatgaaac ggctgcgcac gttcgccaga
    44821 atggtcgtcg ggctcctgag ggaactgagc gacgagagtg catacgagcg ctaccttgca
    44881 gcacacggcc gggagcattc cagggaggaa tggcgccgtt tctccgagaa acggctgcga
    44941 atgaaatatt cgaagccgaa gtgctgctga gaacgccctt gctttatcag aggcccggag
    45001 gctcatcgtc ccggcgccag gaatacccgt tccgaaaaac agtcaaaatg tgcgaagctc
    45061 cgtccggccc cagttttcgc cggagacgtt ggatgtggac gtcaagtgtc cgcgtgcgcg
    45121 ttccgccacg gtaaccccag atagtttcca gcaggtagcc tcgcgacagg caacgtccct
    45181 cgttcagcct caggatcccg agcagttgtg cctctttccg tgtaagcttc ttgtgttcaa
    45241 agacttggcc gtttcttgcg gcttgcggtt gcattgcgcc accggacgga atggcagagc
    45301 caagcatcac gcggtctcct ttacatttgt atctactgta tctacggacg gccaacggaa
    45361 acgtcacgga gaaacgtcgc tgaccacgat cgcccactca agccgcaact tggacgatcc
    45421 tgctcccgga gacatctacc gccaaaagcg aggcgttttc ttttccttgg cgagaacggt
    45481 tagcacggga cgataactaa aggactaaaa gaggtagccg aaagataggg gccgtggttg
    45541 gaatccggtg ccttattctt gggtgtgttc tgttcgaccg ccgttgagag aagacgcgga
    45601 aagcgttacc tgcttcgttt ggcctgccgt gtgacctcgc cggacgagcg ctttgcggag
    45661 attcagggag cgacgaccga catcagccga accggggtcc gcgtcgtctt caaggacact
    45721 gctgcagtgc cggcatttcg catcggggag accgtatgcg tcctgatgga cctgccccaa
    45781 agcccctact tcaccccgcg tgtactcgag tgcatcggca gagtggtgcg ggtcggagac
    45841 accgatccgg cggggccggc ggtcgctttc gaaattgttc ggacccgcgt tcgggagcgt
    45901 gacggacggg tggtcgaagg agatgccaca gcgagcttgc tgcagtgaac gctgggcctg
    45961 ttattctggc ggattcgatt tatgcggagc gggccttggc agccgcgtcc gacgctgccg
    46021 ttcccggtgg tttgccatgc accggccctt cgctgtccgc gggctccact tccacctcga
    46081 ctgcgttccg gtcccgtgag acgaagcgca gaacgctgga cactggaatg ctgacaagta
    46141 acggcgcctc ggtcacgggc tccgcgaaca acggcgcccg gacacgctgt gtcgcgatag
    46201 cggctgccgt tgcggaggac gtgaaaccca caatcaggat cgaaccttcc ggagtcttgt
    46261 gaatctcgaa cagccctggc tgtttcgccc gaagaaccgg tgaatcgcgg ctggccggca
    46321 gcatggtgaa accgtacgcg ccatctggga gcttgccaaa gccgaaccca tcctccagtt
    46381 gtgtcaacgg tctcaccttg tgccgtgccc gcagggcttg gcgcttcgct tccaccggat
    46441 ccaatccgtc gtcgatcctg gccgctaccg gcgccttggg cgcgaggctg ccacgacgca
    46501 tcaccatcag gcaaatcagc gccgcaatca gaaagatcag cagccagata agagcgacta
    46561 tccagcttcg gtaggccccg atggacgcaa tactggagct aacaactgcg aagatctcct
    46621 ccatgccgtc tgcgctccga tgcctcgcgg tccagcccta acggacgatt cgggccggtt
    46681 ccttgcgggc ggtccgagaa acacggacaa gccggccccc acctgaactg aggtgtgtgg
    46741 atttcttgta tgagcaccgg ctacccagat tatggacgcg cgcccggcgc tcgtcaaggg
    46801 ccagaaaaga atccggcgaa gaattgtacc gcgcgagaga tcatgccctt caatgcggat
    46861 atactggtct gtttcgaaag gagagttaag ttgatgacgc ctataaccag aagggggatg
    46921 ctgggactct cagggctgct cgccaccacg tgctggagga gcggcgctct ggcaaagcct
    46981 ctcggtctgc ccatcggcct acagccctac accgtccgaa gggaattggc acaggactgc
    47041 gagggtacac tgcggaagat cgccgcgatc ggttaccagg aactcgaaat cggcgacccg
    47101 ttctacggac aagagaacga tggacttcgt aagttactga aatcactagg catgacgacc
    47161 tcgtctgccc actatggcta tccgaaggac ggttctgagt gggccaagag catcgagcac
    47221 gccaagatgt tcggcataaa gtacatgatc acatcggcgc cctcggagtg gcgcaagagt
    47281 ctcgacggct ggaagcgggc tgcggagcgg ttcaatgagc tcggcgaaca atgccgcaga
    47341 gccggggtgg cggccgccta tcacaaccac catttcgagt acgtggtact cgacggcaag
    47401 gtcgcttacg acgagttcct gaggctgacc gaccccaagc tcgtcttcat ggagctcgac
    47461 tgtttctgga ccacctttgc cggcaaggat ccggtcgcgt acatggagaa gtacccgggc
    47521 cggttcccgc tgctgcacat caaagacctc aaaccggggt ttccgccgag cacggacgcg
    47581 gtcaaaggga agccgtacgc cgaggttggc aaaggaatca tcgactggaa gcggatcttc
    47641 caggcggcgc ccaaaggcgg cattcggttt tactacgtcg agcaggacga atgcgaccgg
    47701 ccgcccattg agagcatccg gatcagccac gactacctga atgaactgag cgtttagcca
    47761 aactgcggat cgcggtaaca tcgggaggcc gcgatacgcc ttatgtgcat gacacccagc
    47821 aggattcttg ttgttttcac cctcgttgcg gttatggcgg gcatcactct tgccgccgac
    47881 gtgaccggaa agtggaccgg ccaggttccg ggccgcgacg gccagatgcg cgaggtgacc
    47941 tacacattca aggtggacgg agagactctc accggcagca tgacgggctt tcgcggtcag
    48001 gatcttccga tctccgacgg caagatcagt ggtgacacgc tgtccttcgt cgtgaaggcc
    48061 ga
//
LOCUS       DATIFQ010000537        17327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_952883, whole genome shotgun sequence.
ACCESSION   DATIFQ010000537 DATIFQ010000000
VERSION     DATIFQ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 17327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17327
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952883"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VLE22_27035"
     CDS             <1..940
                     /locus_tag="VLE22_27035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18137.1"
                     /translation="WSAHAATFTTFDVPGVRGSLVQSISPAGEITGSYVDASFVVHGF
                     LRARDGTLTTFAAPGANFTKAVSINPAGEITGSYVDGSFVSHGFLRARDGTLTTVDVP
                     GANFTTAVSINPAGAITGSYVDASFVLHGFLRGPDGTFATVDVPGANFTTAVSINLAG
                     AITGSYVDASFVLHGFLRRPDGTFTTFDVPGAVHTYPVSINPAGAITGYYPDASYAFH
                     GFLRARDGTLTTFDVPGAVHTYPASINPSGAITGSYMDGSFVVHGFLRAPDGTLTTFD
                     VPGAVHTYPASINPAGVITGSYVDASVVLHGFLRSP"
     gene            complement(1273..3567)
                     /locus_tag="VLE22_27040"
     CDS             complement(1273..3567)
                     /locus_tag="VLE22_27040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18138.1"
                     /translation="MGSNSEGTMNLRMGARILAATFLLSAVAGAQVKVSEIRIKTDPE
                     NATIRPFETAVLQVLIYGEPPDLEGKPKRVRLQGSAEFQVADRGGGWLSRPFRYQGSE
                     DEPFYGDQQEGLRRILSRTQETFILQDAVLYTAPERPGKYQVQASREGKTATVTIRVD
                     PGAPSYRKPEKVSFPSEPLSRDKYRKWAEHYAPFIAQETWFQPKADFLARFDYDGDWR
                     GDNNWDALNEGASQAYVYYTVMETQTHWFLIYNLFHPRDYSDKCVAGSCHEDDNEGLI
                     LTVRQDGSAFGRLETMETLAHNNIYSYRADRRVQDGVHDIDGNVELLDQSHPVVFVEA
                     GGHGIYGSQDSHSRYSLERDEFTASTGVTYLYTGASEKPRCADDRRVGYDLLPIYEQW
                     WLRAQEGSGRNDRTFDEYFVYQPLGGRPGTSYEKIAGSFLGRKEASNKAKPFWGWHDE
                     RTQKQNILATGQWGLDPAYAVSRNLRFSADEPFSLEYVFNPYLQTAAPGAAPVLAPAV
                     ITEATFQEIIPRQSGNYDPNASEGQLDLRLYVDGTVEVSLQGDRIRYEVRSGRPPRDD
                     GSELTQPIPRGTFRFFGMEQKGGREKVTLLEQPSQDNDYTVRLRIEDSKVGEGRYHAR
                     LRWKWEAMAPASANPRYPGHPPAGTELTSTDNDPSKYKQDRQGLFEFRGRVDGVVVLR
                     IRGDRGYAEVLSGAPVQVERFSFSQPLPSVPLKEIKVEKKDGRGEVTLLDQPSIANDN
                     TAAVQVTDLKGGAGRYHFRLTWRR"
     gene            complement(3601..4290)
                     /locus_tag="VLE22_27045"
     CDS             complement(3601..4290)
                     /locus_tag="VLE22_27045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18139.1"
                     /translation="MRLNAMVHVGLALVAVLVQAGCNKDTGPTQDVVAPEKTTGARGG
                     DSGVTRTAKLPANTVTLPEGTPLTVRTTTALSTNTQEAGQTFTAHLEKPLVYGGREIA
                     PNGAVVQGRIVEADKGGRVKDRASLAVQLTRLHTAGGHVVEISTSTITREARATKGKD
                     AAKIGIGSGVGAAIGAIAGGGKGAAIGAAAGAGAGTGVVLATHGDPAVIPSESVLDFA
                     LSAPVTVAELR"
     gene            complement(4493..4669)
                     /locus_tag="VLE22_27050"
     CDS             complement(4493..4669)
                     /locus_tag="VLE22_27050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18140.1"
                     /translation="MLGYEIGNAFTSGDPPRPFNDGVVGLIRETPVGVVFFGGSFGER
                     GEKKVFFTVGRFSY"
     gene            complement(4736..6139)
                     /locus_tag="VLE22_27055"
     CDS             complement(4736..6139)
                     /locus_tag="VLE22_27055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSB18141.1"
                     /translation="MRLYLVNPDNPVVSLNKVHWNRLNTYRVWKPLGLLVVAGLTPPD
                     WEVTLIDENLSHADYGLLPKPDLVGITAFTSQAPRAYEIAGMYRAMGVPVVMGGIHAT
                     FCCDEALEHADAVVTGEAESVWGQVLADARSHALRRVYQGRISAMDAIPRARHDLLHG
                     RYYFGSVQTTRGCPLNCSFCSVTAFNGGTFRHRPVTDVIRELRLIREKVILFVDDNLI
                     GTRRDHLEHSKELFRAMIREGLTRPWICQATINFADDEELLDLASRSGCQGVFIGFES
                     STVEGLMSVGKKFNLQKGRDFRESVRRIQRHGMCVVGSFIIGIDTDQRGIGETIARAA
                     REYGIDAANVLILTPLPGTKLYAQMEREGRIRSNDYPRDWKYYTLTYPVADYRNLTWA
                     EVVEEVNRFNDRFYSYPQILRRMLRLARNTRSLTTFLVVLVANLSYRSNHLLDRRIHA
                     SRVHFPESGDILAAEPG"
     gene            complement(6156..7019)
                     /locus_tag="VLE22_27060"
     CDS             complement(6156..7019)
                     /locus_tag="VLE22_27060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18142.1"
                     /translation="MKTNTRTAIRATMRALLLGLASATALLGQVDAREMIRRAVAADE
                     SNWRVARNYMFSERVDLRYLDSLGRVKSKEVETFDVMLLDGSPYKRLAARDDRPLTPG
                     EESKEQKRLARSADHRRKETASQRAQRLAEYEKRPEWQREAWRELPEAFDFRLAGEEL
                     WNGNSLYVIEATPRQGYQPRSRTAEILTHLQGKLWVDKQDYRLVKAEAEVVDTISVGL
                     FLVRVAKGSRAAFELTRVNDEVWLPLQVRAFVSARLGLLKVLHVEQEISYSKCREFQT
                     DSPIISQMKAR"
     gene            complement(7192..7473)
                     /locus_tag="VLE22_27065"
     CDS             complement(7192..7473)
                     /locus_tag="VLE22_27065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HSB18143.1"
                     /translation="MDRRITNMEKVEMNGNRAYALPLFLAGLVTGIAVTVLLAPRSGA
                     ATRRLVGRKVEEGENWMKAKAAAAQGYVSSRGAELRDRVKEAAEVLGPS"
     gene            complement(7576..8199)
                     /locus_tag="VLE22_27070"
     CDS             complement(7576..8199)
                     /locus_tag="VLE22_27070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB18144.1"
                     /translation="MNQVETEVRAAGTSWRWGAETPDHAVLERVRAGETAPYEVLVHR
                     YNPRLRRVVRRILSNEADVEEVMQETHYNALRFIRQFAGRSSFVTWLTRIAIHAALSR
                     VRRRAYLHELSPICCTGEPLETVVSAERDPEQQLRDKETREGLEAAVRALPESYRAVL
                     LLRRFEELTTTEAAALLEISEGCAKTRLRRAQALLRSRLQVAPAPSG"
     gene            complement(8355..8936)
                     /locus_tag="VLE22_27075"
     CDS             complement(8355..8936)
                     /locus_tag="VLE22_27075"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HSB18145.1"
                     /translation="MARKNTAVLGVYPNYSSVENAVDVLKEAGFRNTDISVLFPEKAG
                     SKDFAHHKGTKAPEGAAAGAGTGAVLGGALGWLVGIGTLAIPGLGPFIAAGPIMAALA
                     GMGVGGAVGGIAGALIGMGIPEHEAKRYEVRVKEGGILLSVHSDNADWTRRAKEILER
                     TGAQDVSSTGKAGADYVRSDRPHARTTTGGGTI"
     gene            complement(9141..9293)
                     /locus_tag="VLE22_27080"
     CDS             complement(9141..9293)
                     /locus_tag="VLE22_27080"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HSB18146.1"
                     /translation="MLWTICVILFVLWALGMVTSYTMGGFVHVLLVLALVMIAVNLMR
                     GRRSIV"
     gene            complement(9321..9926)
                     /locus_tag="VLE22_27085"
     CDS             complement(9321..9926)
                     /locus_tag="VLE22_27085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18147.1"
                     /translation="MKRLLGLLAVATLAVAQVRTIGTGTSITVRTNETIDVKKSDGRV
                     FSGVVNQDVMDANGTVAIPTGSNAELIVRKASNKDLTLDLESVTVNGQRYAVTADASR
                     LSGGQRDGIGKNKRTAEFIGGGAALGAIIGAIAGGGKGAAIGAAAGAGAGAGTQVLTR
                     GRTVKVPAESLLTFRLEQPLEVGAVDHGITRNGRHYHTRYR"
     gene            complement(9946..11883)
                     /locus_tag="VLE22_27090"
     CDS             complement(9946..11883)
                     /locus_tag="VLE22_27090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSB18148.1"
                     /translation="MIDSRKFHDSVLKTGPSVAVVAGIVWALCLPYQAGAQGGFNGPG
                     RYEITNLKSGKVLDLDRNDQTSVIQFSSRGTDNQAWEIRAAGGGFYSLRNVMNGNALE
                     AVGTRNSTPVRATAFSGGSSQQWRFDTGKDGTALIVSRLGKTLDIPGGTSSDGARVQI
                     YDSNGESNQQFTFRGVSGNQGGASSGNVRPNYVTPNSGAGRTQLKPGWNMFSPQQDVE
                     VGQQVSKDAEQQIPMLKDSRVDNYLNNLGQRLSAHAPGYKFPYAYKAVNDRAINAFAL
                     PGGPVYINRGVIEAADNEAQLAGVMAHEASHVALRHGTNQASKASAAQMPLSILGGML
                     GSNSTGAVLAQLGAGFAVNSVLLKYSRTAETQADVMGSQILYDSGYDPRAMAQFFEKI
                     QAQDKSGQPVAFFSSHPSPDRRTERVNEEVANLGGSQRGYNTDSREFDQIKRYAQSLP
                     APRPNQLQSQQQQGTPGRPDWPSDRFVSFENSLLRMNRPDNWQAYGQGDAATIAPRGG
                     LVDDGKGNQALAYGVIVNIFEPPRVDQYGRQLQGPAQNSGTYLAEATNQLVQEFRLSN
                     RNMRVVRSHEDIDVNNVRGLSTYLSNDSPIQGGGRETNWLITLPRPEGLLFLVFTAPE
                     RDFQSYENTFQQMLYSVRVDR"
     gene            complement(12020..12211)
                     /locus_tag="VLE22_27095"
     CDS             complement(12020..12211)
                     /locus_tag="VLE22_27095"
                     /inference="COORDINATES: protein motif:HMM:NF041949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HSB18149.1"
                     /translation="MPLVNLVIALIVVGVALWLINRFIPMASSIKTILNVVVVVAVGV
                     WVLQAVGVWGHMSGYRIGR"
     gene            complement(12512..12922)
                     /locus_tag="VLE22_27100"
     CDS             complement(12512..12922)
                     /locus_tag="VLE22_27100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSB18150.1"
                     /translation="MNCVRTGIAFGPRAEQRRTASSADVMQAPAAPPDWFPIVREVRY
                     KVRNSKGVVEIGTGETINISSTGVLFNAQAPLPPGKRIELSISWPVQLDGKCGLKLVA
                     CGRVVRCEGTNVALKIEKHEFRTTGRGLGARAGI"
     gene            complement(13260..13409)
                     /locus_tag="VLE22_27105"
     CDS             complement(13260..13409)
                     /locus_tag="VLE22_27105"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HSB18151.1"
                     /translation="MLWTLAVVLFVLWVLGLVTSYTMGGLLHLLLVVALVVVVLQIIQ
                     GRRIA"
     gene            complement(13688..14068)
                     /locus_tag="VLE22_27110"
     CDS             complement(13688..14068)
                     /locus_tag="VLE22_27110"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSB18152.1"
                     /translation="MKIAHSEMRRFQKILERKEAELVQVLRKRDGIAIEKSADQMDEI
                     QYASERDLAIRNVDSQSTLLRDVKAALRRIHDGTFGTCIECAWAISPKRLAALPWAPL
                     CIECQEAADRDKYEATEYLVDALS"
     gene            complement(14301..>17327)
                     /locus_tag="VLE22_27115"
     CDS             complement(14301..>17327)
                     /locus_tag="VLE22_27115"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HSB18153.1"
                     /translation="YREKMIKRRILRRLALRNIDGLAEYGKRLENDSGELMALQRDLL
                     IGVTSFFRDRESFERLKKAVFPLILQGRSANETIRVWVAGCATGEEAFSIAITLQEHL
                     NETGATFPVQIFASDVSLAAIEKARTGRYLENIAADLTHERLNRYFTKIEGGYQINRD
                     LREMCVFTRHNLIDDPPFSKLDLISCRNVLIYLGSVQKDIIPLFHYALKPTGFLMLGA
                     SEAAASGDLFSVADREHRIYAKRETARKPHLFHAAARGSRRGAPAGSASAAPAPELWG
                     GADVRKEVDRILLSKYSPAGVVVDGDLEVTEIRGKASPYLTLPVGKLSFNLMKLIPDT
                     GLFLDVEKLIQQVRHSGEPARQERVRYEHDGSAGELNVEAVPLDASQKRSILVLFEPV
                     PGAAGRETEPPDAPPEADARDRQISRLKQQLADAKQRFLSAMEEHQLSREESQNTTEE
                     ALSANEELQSLNEELETAKEELQSTNEELITVNDELQAKNAALGQARDFAMSIVETVR
                     QPLLVLDPDLRIRMANRAFYRTFQSSPLEAEGQVIYSLSRGSWDLPGMRDSLDGLLQG
                     GNSFPDFEVEQHFPTVGRRSLVLGGCRINHLKMILLAVDDITERKLAQQALRRSEEHL
                     RQSQKMEAVGRLAGGIAHDFNNLLTAIIGYSELLADTLAGNEPAIQQVLEIKKAGDRA
                     ALLTQQLLAFSRRQVLQPKVLDLNVIVAEFDRMLRRLVGERIRVAINCAPALWQVRAD
                     PGEIGRAIMNLSLNARDAMPDGGTLAIETANVTLPEADASEPDLAPGRYVTMAVRDTG
                     VGMNAEMQAHIFEPFFTTKETGKGTGLGLATVLGIVEQSGGAIRCESEPGRGTVFTIF
                     LPAVAEAVDKGVRPAGGLAEAPKGSEVILLVEDEDMVRVLARKILEASGYVVLDARNG
                     REGLALCETHEGPIDLLVSDVVMPELGGRELVEAALKLRPGMKVMFMSGHTQDVVLKE
                     GVQKGTAFLQKPFTTVGLAQRVRETLDSDASTAGQP"
BASE COUNT         3168 a         5594 c         4998 g         3567 t
ORIGIN      
        1 ctggagcgct catgccgcta cgttcaccac gttcgacgtc ccgggcgttc gcggcagctt
       61 ggtccaaagc atcagcccgg cgggtgagat cacgggatcc tacgtggacg cgagtttcgt
      121 ggtgcacggc ttcctgcggg cccgcgacgg caccttaacc acgttcgccg ccccgggcgc
      181 taatttcacc aaggccgtta gtatcaaccc ggcgggtgag atcacgggat cttacgtgga
      241 cgggagcttc gtgtcgcacg gcttcctgcg ggcccgcgac ggcaccttaa ccacagtcga
      301 tgtcccgggc gctaatttca ccaccgccgt tagtatcaac ccggcgggtg cgatcacagg
      361 atcctacgtg gacgcgagtt tcgtgttgca cggcttcctg cgggggcccg acggcacctt
      421 cgccacagtc gatgtcccgg gcgctaattt caccaccgcc gttagtatca acctggcggg
      481 tgcgatcacg ggatcctacg tggacgcgag tttcgtgttg cacggcttcc tgcggaggcc
      541 cgacggcacc ttcaccacat tcgatgtccc gggtgctgtt cacacttacc ccgttagcat
      601 caacccggcg ggtgcgatca cggggtacta cccggacgcg agttatgcgt ttcacggctt
      661 cctgcgggct cgcgacggca ccttgaccac attcgatgtc ccgggcgctg ttcacactta
      721 tcccgctagc atcaacccgt cgggtgcgat cacgggatcc tacatggacg gaagtttcgt
      781 ggtgcacggc ttcctgcggg cgcccgacgg caccttgacc acattcgatg tcccgggcgc
      841 tgttcacact taccccgcta gcatcaaccc ggcgggtgtg atcacggggt cctacgtgga
      901 cgcgagtgtc gtgttgcacg gcttcctgcg aagcccgtga gcgcgcgcct tagtggccgg
      961 tgtcaactct tgggcgcaat ttcactgccg acttccgcga tccggcttta ttggatagac
     1021 ccccacgcaa cgaatcccct cttacataag gtcgctccgt gacttggatc tgctgctggc
     1081 tgcgaccagg acctggatgc cgctcgcgga acactcggtc gtgggcttac ttctgccgtg
     1141 ccaagcggtg agagcagctt tacaagccgc tccggcgcac cacgattgca gcaaaagagt
     1201 ttagttcggt gtcgggcagg tttcccgtga atacatcgcg gcccaaactg cgaaagtcag
     1261 gctagcgcta ggctagcgcc gccacgttag ccgaaagtga tatcgaccag caccgccctt
     1321 gaggtcggtg acctgcacag cggcagtgtt gtcgtttgcg atggagggtt gatcgaggag
     1381 cgtcacctcc ccccggccgt ccttcttctc caccttgatc tccttgagcg ggactgaggg
     1441 cagcggctgc gaaaacgaga agcgctcgac ctggacgggg gctccgctca ggacctccgc
     1501 gtacccgcgg tcgccgcgaa tgcgcaggac taccacgccg tcgacacgtc cgcgaaactc
     1561 gaagaggccc tggcggtcct gtttgtattt cgacgggtca ttatcggtcg aggtcaactc
     1621 cgtgcccgcc ggggggtggc cgggatacct cgggttcgcg gaggccggcg ccatcgcctc
     1681 ccatttccag cgcaggcggg cgtgataacg gccttccccg accttggaat cctcaatgcg
     1741 gagccggacc gtgtagtcgt tgtcttgaga cggctgctcc aacagggtta ccttttcgcg
     1801 tccacccttt tgctccatgc cgaagaaccg gaaggtccca cgaggtatcg gctgcgtcag
     1861 ttccgagccg tcatcccgtg ggggccttcc gctacgaacc tcgtaccgaa tgcggtcccc
     1921 ctggagggat acctcgaccg ttccgtcgac gtagagccgg agatcgagct ggccttcact
     1981 agcattcgga tcatagttcc cactctggcg cggaatgatc tcctgaaagg ttgcttccgt
     2041 gatcactgcc ggcgccaata ccggcgccgc ccccggagcg gcggtttgga ggtacggatt
     2101 gaaaacgtac tccagggaaa aaggctcatc cgccgaaaag cgcagatttc gcgagacagc
     2161 gtaagccggg tctaaccccc actgtcccgt ggcaaggatg ttctgctttt gggtacgctc
     2221 gtcgtgccag ccccagaagg gcttggcctt gttcgatgct tccttgcggc caagaaagct
     2281 gccggcaatc ttctcgtacg aggttcctgg ccttccgccc agaggctggt agacgaaata
     2341 ttcgtcgaag gttcgatcgt tgcggccgct accctcctgc gccctcaacc accactgctc
     2401 gtagatgggc aacagatcgt agccaacccg gcggtcgtcg gcgcacctcg gcttctcgga
     2461 ggcgcctgtg tacaggtagg tcacccccgt gctcgcggtg aattcgtccc gctccaggga
     2521 ataccgcgaa tggctgtcct gggaaccgta gatgccgtgg ccgccggcct cgacgaacac
     2581 caccgggtgg gactggtcga gcagctccac attgccatcg atgtcgtgca cgccgtcttg
     2641 cacgcggcgg tcggcgcgat aggagtagat gttgttatgg gccagcgttt ccatggtctc
     2701 cagccgcccg aacgccgatc cgtcttgccg caccgtgagg atcagtccct cgttgtcgtc
     2761 ctcgtgacag gagccggcca cgcacttgtc cgaataatcg cgcgggtgga acaggttata
     2821 gatcaggaac caatgggtct gggtctccat gacagtgtag tagacatagg cttgcgaggc
     2881 gccttcgttg agcgcatccc agttgttgtc gccccgccaa tcgccatcgt agtcaaaacg
     2941 cgcgaggaag tcggccttgg gctggaacca ggtttcctgc gcgatgaacg gtgcgtaatg
     3001 ctcggcccat ttccggtact tgtcccgcga caaaggctcg gagggaaacg agaccttctc
     3061 cggtttccgg taggaaggcg caccgggatc cacccggatc gtaacggttg ccgttttgcc
     3121 ctcccgagaa gcctgaacct ggtattttcc cggccgttcc ggcgcggtgt aaaggacggc
     3181 gtcttgtaag atgaaggtct cttgggttcg gctcaggatc cggcgcaacc cctcctgttg
     3241 gtctccgtag aacggttcgt cctcgcttcc ctgatagcgg aacggtctcg acagccagcc
     3301 tccaccccgg tctgctacct gaaactcggc gctaccctgg agacgaaccc gcttcggttt
     3361 gccttcgagg tcgggcggct caccatagat caacacctgc aaaacggcgg tctcgaaggg
     3421 ccggatcgtt gcgttctccg gatcggtttt gatccttatt tccgacacct tgacctgagc
     3481 gccagcgacg gcgctcaaca ggaaggttgc ggcgagaatt ctcgccccca ttcgcaggtt
     3541 catcgtcccc tctgaattcg agcccatggg tcctccagcg gtgtcaataa tgaagacctt
     3601 ctacctcaac tcggcgacgg tgaccggggc gctaagcgca aagtccagca cgctttcgct
     3661 cgggattacg gcgggatcgc cgtgtgtggc tagcaccacg ccggtccccg ccccagcgcc
     3721 ggcggccgcg ccgatggccg cacccttccc gcctccggca atggccccaa tggccgcgcc
     3781 tacgccgctg ccaattccga ttttcgcggc gtccttgccc ttggtggctc tcgcctcgcg
     3841 cgtaatggtg ctggtcgaaa tctcgacgac atgaccgccg gctgtatgca gccgcgtcaa
     3901 ctgaacagcc aggctggcgc ggtccttcac ccgcccgcct ttatccgcct cgacaatcct
     3961 gccctgcacc accgcaccgt tcggagcgat ctcgcgaccg ccataaacca ggggcttctc
     4021 cagatgcgcg gtgaacgttt gccccgcctc ctgcgtattg gtggacaaag cggtcgtggt
     4081 tcgcacggtg aggggggtcc cttccggcag ggtcacggta ttagccggaa gcttcgccgt
     4141 gcgggtaact ccggagtcgc cgccccgagc gccagttgtc ttctctggcg ccacgacatc
     4201 ctgggttggc ccggtatcct tgttgcatcc tgcctggacc agaacggcca cgagggccaa
     4261 gcccacgtgc accatcgcgt tcaatctcat atcaacatct ccttcggcgc gccatcaggt
     4321 ggccagttcg gcaggctgcg ccaaatcaca atccgagcgg aaacgctcct gacaggcgac
     4381 gcaacggact cctcttggaa tcgcctcgag gcgcctaccg gaaatgacgg agcacgtcgc
     4441 ctgccaaagg tgcgcttatg ctcaagagaa aaatatccgg tcgcagcgct catcagtaag
     4501 agaaccgtcc cacggtgaag aagaccttct tctcgccgcg ctcgccgaag ctgccgccaa
     4561 aaaacacgac tccgacgggg gtttccctga tcaggccgac cacaccgtcg ttgaacggtc
     4621 gcggtggatc gccagaggtg aacgcgttcc cgatttcgta gcccagcatc gcccgaaaac
     4681 tgctcgcgaa cgacagcttc tccttagaca acggagtgct ggaagggaca ctgtctcaac
     4741 ccggttccgc cgccaagatg tctcccgatt cgggaaaatg aacccggctg gcatgaatac
     4801 gcctgtcaag gaggtgattg gatcggtagc tcaggttggc aaccaggacc acaagaaacg
     4861 ttgtgagact gcgagtgttc ctggctagcc gcagcatccg gcgaaggatc tgcggatacg
     4921 agtaaaagcg atcgttgaac cggtttacct cctccaccac ttccgcccag gtaaggttcc
     4981 tgtagtcggc caccggataa gtcagagtgt agtacttcca atcccgtggg taatcgttcg
     5041 accggatcct cccctcgcgc tccatttgag cgtagagctt ggttccggga agcggagtaa
     5101 ggatcagaac gttggcggcg tcgatgccgt attccctggc tgcgcgagcg atcgtctcgc
     5161 cgatcccccg ctggtctgta tctatcccga tgatgaacga gcccacaaca cacatgccgt
     5221 gacgttgaat ccggcggacc gactcgcgga agtctctccc cttctggagg ttgaacttct
     5281 tgcctacgga catcagccct tccacggtgg aggattcgaa cccgatgaat acaccttggc
     5341 atcccgaacg ggaggcgaga tcaagcagtt cctcatcatc cgcgaagttg atcgtggcct
     5401 ggcagatcca aggccgggtc agcccttcac gaatcatcgc tcggaagagt tccttggagt
     5461 gttcgagatg gtcgcggcgg gtaccgatga ggttgtcgtc cacgaagagg atgacctttt
     5521 cccggatcag ccgcagttct cgaatgacat cggtgacggg ccggtgtcgg aacgtacctc
     5581 cgttgaacgc ggtgacgctg caaaagctgc aattcaacgg acagccgcgc gtggtttgca
     5641 ccgagccgaa gtagtaccgt ccgtggagca ggtcgtggcg agcgcgcgga attgcatcca
     5701 ttgcgctgat gcggccctga tacaccctgc gcagggcatg gctacgcgca tccgccagaa
     5761 cctgccccca taccgattcc gcctctcccg taaccaccgc atccgcatgt tccagcgctt
     5821 catcgcaaca gaaagtggca tggatgcctc ccatcaccac tggcactccc atcgcgcggt
     5881 acattccggc gatttcgtaa gcccgcggcg cctgggacgt gaaggccgtt ataccgacca
     5941 gatcaggttt gggcaggagt ccgtagtcgg cgtggctcag gttctcgtcg atcagtgtca
     6001 cctcccagtc cggcggcgtc agtcctgcca ccacgagaag cccgagcggc ttccagactc
     6061 gatacgtgtt cagacggttc cagtggacct tgttcaggct gaccactgga ttgtccggat
     6121 tcacaagata gagtcgcatc tatttcctat ccggtttacc tagctttcat ctgcgagatg
     6181 atcggggaat cggtctgaaa ctcgcggcac ttgctgtagc tgatttcctg ttcgacgtgc
     6241 agcactttca gcagtcccag acgggccgag acgaatgccc gcacttgaag cggcagccag
     6301 acctcgtcat tcactcgggt cagttcaaag gccgcgcgtg agcccttggc cacgcggacc
     6361 aggaacagcc ccaccgagat ggtgtcgacg acttccgcct ccgctttcac caagcggtag
     6421 tcctgcttgt ccacccaaag cttgccctgg agatgggtaa gtatctccgc cgtgcgggac
     6481 cggggctggt atccctggcg cggtgtagct tcaatcacgt acaggctgtt gccattccac
     6541 agctcttccc cggccagccg gaagtcgaaa gcttccggca gctcgcgcca agcctcgcgc
     6601 tgccactccg ggcgcttctc gtactcggct aggcgttgcg cccgctgcga tgcagtctct
     6661 ttccgacggt ggtcggcgct cctggcgagt cttttctgct ccttactctc ttcgcccggc
     6721 gttaacgggc ggtcgtcacg cgccgccagt cgcttgtagg gcgagccatc tagaagcatg
     6781 acgtcgaagg tctcgacctc ctttgatttc acccggccca gggagtccag gtaccgcaga
     6841 tccacccgct ccgaaaacat gtagttccgg gccaccctcc aattactctc gtcggctgcg
     6901 acggcgcgac gaatcatctc acgggcgtcc acctggccca gcaaggctgt cgccgaagcg
     6961 aggcccagca gcagcgcgcg catcgtagcc cggattgccg tgcgggtgtt cgttttcacg
     7021 cgcgcggttc ctagtagggt tgcggtgtgc cggaggggat ggccgttgtt cttcacgggt
     7081 cacttctcct tcttacctgg aatctccggt tgtccggttg ttcaaaccac gcgctgcaat
     7141 gcggccccgg ccgtttcggg cgactcacct tcttgcgcgg agcggggcct ctcagcttgg
     7201 cccaagcacc tcagccgcct ctttgacccg gtcccgcaac tccgctccgc ggctgctgac
     7261 gtagccctgc gccgcggcag ccttggcctt catccagttc tcgccctctt cgaccttgcg
     7321 tccgacgagg cgacgcgtcg cagcgccgga gcgtggggca agcaagaccg tcaccgcgat
     7381 gcccgttacc aacccggcca ggaacaaggg cagtgcataa gctctattgc cgttcatttc
     7441 aactttctcc atgtttgtga tccttctatc catgtagcaa tcctgtgacc gcacttattc
     7501 atggaggccc ttgcgagcga accctgtatc cgctacttcc atttcgaccg gccagacgtc
     7561 gcgaatcgca ttcgctcaac cagatggcgc cggcgcaacc tgaaggcggc tgcgcagcag
     7621 cgcctgggcg cgtcgcaggc gcgtcttggc gcacccttcg ctgatttcga gcagcgcggc
     7681 cgcttcagtc gtagtcaact cttcgaacct gcgcagcaaa agaacggcgc gataggactc
     7741 cggcagcgcc cgcacggcag cttccaaccc ttcgcgcgtt tccttgtccc gcaactgctg
     7801 ctcgggatcc cgctcggccg aaacgacggt ttcaagcggt tcgccggtgc agcagatggg
     7861 actcaattca tgcaagtacg cccggcggcg cacccgagac agcgcggcgt gaatggcgat
     7921 ccgagtcagc caggtgacaa agctcgaccg gcccgcgaac tgccggatga agcggagagc
     7981 attgtagtgc gtttcctgca tcacctcttc cacgtcggct tcgttggaca agatgcgacg
     8041 cacgacgcgg cgcaaacgcg gattgtagcg gtgcaccagc acctcatacg gtgcggtttc
     8101 cccagcgcgc acgcgctcca ggaccgcgtg atcgggcgtt tccgcgcccc agcgccagga
     8161 ggttccggcg gcgcggactt ctgtttcgac ctggttcatc cctcccccct cattgcgagc
     8221 cgcgaaagct agccatacat ggtcatgtga ccgggcatgg tcaaagatcc ttgccagata
     8281 tccccacgca tgaccggagc ttgccgggct atccgggccg cgtacagcat ctgcccactc
     8341 ctccgatcag cgtgctatat cgtcccaccg ccagtggtcg tgcgagcgtg cggcctatcg
     8401 cttctgacgt agtcggcgcc agcctttccg gttgacgaga catcctgcgc tcctgtccgc
     8461 tccagaatct ccttagcgcg ccttgtccag tccgcattgt ccgaatgcac ggagagaagg
     8521 attccgccct cttttacccg gacctcatag cgcttggcct cgtgttcggg aatccccatg
     8581 ccaatcagtg cgcccgctat cccgcctacc gcaccgccta cgcccattcc tgcaagggcc
     8641 gccatgatag gaccggcggc gataaaggga cccaaacccg gtatcgccag ggtgccaatg
     8701 ccgaccaacc atcccaatgc acctcccagc accgctccgg ttccggcgcc cgcggcagca
     8761 ccctctggag ccttggtgcc tttgtggtga gcgaagtcct tggagcctgc tttttccgga
     8821 aatagtacgg aaatatccgt attccgaaat ccagcctcct tcaagacgtc gacggcgttc
     8881 tccacgctgg agtaattggg gtaaacaccc aacactgctg tgttcttccg agccatgttg
     8941 cacttctcct tatctggtct taagttcaag ttcagaaccg cgaccgctac aataaaatgg
     9001 atgcctcgcc gccaccggta gtgtggcggt cgtcatccaa caatcccccg cttctgttgt
     9061 cgccccctcg ccatgctgtc aggacgctcg aaacacttcc aaaggtcaca tgtttcagga
     9121 gtgactttca ccacgggctg ctagacaata gaccggcgtc cccgcatcag attgacggcg
     9181 atcatcacca acgccaggac cagcaacacg tgcacaaagc cgcccatggt ataggaagtg
     9241 accattccca gcgcccacag cacaaacaga attacgcaaa ttgtccacag catgttctta
     9301 cactcctttt gtctatcctg ctaccgatat ctagtgtgat aatggcgccc gttgcgagtg
     9361 atcccatgat ccactgctcc cacttcgagc ggctgctcca gacggaacgt cagcaaggac
     9421 tcggcaggaa ccttcacagt cctgccgcgg gtcaggacct gcgtgccggc tcccgctccc
     9481 gcccccgccg ccgcaccgat cgcagcgccc ttgcctccgc cggcaattgc tccaatgatg
     9541 gcgcccagcg ccgcgccgcc gccgataaac tcggcggtcc gcttgttctt cccgataccg
     9601 tcccgctgtc cgccactcag gcggcttgca tccgcggtta ccgcataccg ctgcccgttc
     9661 acggtaaccg attccagatc gagcgtcaga tctttattcg aggccttcct cacgatcaac
     9721 tcggcgttcg atcctgtggg gatggcaacg gtgccattgg catccatgac gtcctgattc
     9781 acaacgccgg agaacacccg gccatcgctc ttcttgacgt cgattgtttc gttggtccgc
     9841 accgtaatac tggttccggt gccgatcgtt ctcacttggg ccacagccag cgtcgccacg
     9901 gcgagaagtc ccaatagtct cttcatgatg aatctctttt gccggttacc gatccacgcg
     9961 aaccgagtac agcatctgct ggaacgtgtt ttcgtagctc tggaaatcgc gctccggggc
    10021 tgtgaagacg agaaacagca ggccctcggg gcgcggcaga gtgatcagcc agttggtctc
    10081 gcgacccccg ccctggatgg gcgaatcgtt cgagaggtac gttgaaagcc cgcgtacgtt
    10141 gttcacgtcg atgtcctcgt gggagcgaac cacacgcatg ttccggttgg acaggcggaa
    10201 ttcctggacc aattggttgg tggcttctgc caagtaagtc ccggagttct gcgcggggcc
    10261 ctgcaactgc cggccgtatt ggtccacgcg cggcggctcg aagatgttca cgatgacgcc
    10321 gtaagccagg gcctgattgc ctttgccgtc atcgacgagc ccgcctcttg gtgcgatggt
    10381 cgcggcgtcg ccctgcccgt aggcttgcca gttgtccggg cggttcatgc gcagcagcga
    10441 attctcaaaa ctcacgaagc gatccgaggg ccagtccggc cggccagggg tgccctgctg
    10501 ttgctggctt tggagctggt tgggtcgcgg cgcaggcaaa gactgggcgt agcgcttgat
    10561 ctgatcgaat tcccgagaat cggtattgta cccgcgctgc gagccgccaa ggttggccac
    10621 ttcctcgttc acgcgctcgg tgcggcggtc cgggctgggg tggctgctga agaacgcgac
    10681 gggctgcccg ctcttatcct gcgcctgaat cttctcgaag aattgagcca tggcccgcgg
    10741 gtcgtagccg gaatcgtaca ggatctgcga gcccatgacg tcggcctggg tctcggctgt
    10801 gcgcgagtac ttcagcagga cggaattcac cgcgaagccc gcaccgagct gcgccaggac
    10861 ggcccccgta gagttgctgc ccagcatgcc acccaggatc gacagcggca tctgcgcggc
    10921 ggaggccttg ctggcctggt ttgtgccgtg gcgcaacgcc acgtgggagg cttcgtgcgc
    10981 catcactccg gccagttgcg cttcattgtc ggcggcttca atcacgccgc ggttgatgta
    11041 gacgggcccg ccgggcaggg caaaagcgtt gatcgcccga tcgttcacgg ccttgtacgc
    11101 atagggaaac ttgtagccgg gcgcatgcgc cgaaagtcgt tggcccaggt tattcaggta
    11161 gttgtccacc cgggagtcct tcaacatggg aatttgctgc tcggcgtcct ttgaaacctg
    11221 ctggcccacc tcgacgtcct gctgcggcga gaacatgttc cagccgggct tgagctgtgt
    11281 gcggccagcc cccgagttgg gggtcacgta gttgggacgc acgttgccgc tcgacgcccc
    11341 gccctggttt ccggacactc cccgaaaagt gaattgctgg ttcgattcgc cgttgctgtc
    11401 gtagatctgg acccgcgctc cgtccgagct cgtaccgccc ggaatatcca gcgttttgcc
    11461 gagtcgcgag acgatgagcg cggtaccgtc tttgcccgta tcaaagcgcc actgttggct
    11521 ggaaccgccg ctgaaggccg tggcccgcac gggcgtgctg ttgcgggttc caacggcttc
    11581 gagcgcgttt ccgttcatca cgtttcgcaa ggaatagaag ccgccgccgg ctgcccggat
    11641 ttcccaggcc tgattgtcgg ttccccgcga ggagaactgg atcacgctcg tctggtcgtt
    11701 ccggtcaaga tcgagcactt tgccggattt caaattggtg atttcgtacc ggcccgggcc
    11761 attaaacccg ccttgcgcgc ccgcctgata tgggaggcac agcgcccaga cgattccggc
    11821 taccacagcg accgatggcc ccgttttgag aactgaatca tggaacttgc gactgtcgat
    11881 catgttcatc tcctatctgc ctccgcgcgg cgagtgcttt tccacacgcc aaacccactt
    11941 catgccatcg tgtagtcgag acgcgccggt cttggccaat cccgcgcgat ctaccgcgcc
    12001 atcgccggaa cgcaaccttt cagcgcccaa tgcggtagcc gctcatatgt ccccacacac
    12061 cgactgcctg caatacccaa acgccaacgg caaccacgac gactacgttc agaatcgtct
    12121 tgatgctgga ggccatggga ataaaacgat tgatcagcca caacgccacg ccgaccacga
    12181 tcaacgcaat aacgagattt accaaaggca tgttcttaca ctcctgaaag gtacgtagca
    12241 cttggctgac caatcgccgg aagcgaccct acacgaagaa agtggtggac tcgccagggc
    12301 cccgcagcga ctccggcggc ccagcgggaa gatcagtcct cgcttctcca ggtagggaga
    12361 agaacctgga accgcatcgc tgaagaagga aaacctgatg atgtacatct tctagtccgg
    12421 aatcggggcc aagaaactcg aaggacgggg ccgaacggct ccgtcctttt tcactcttgg
    12481 gctttggttt tctgccccgg ggtcggggcg atcagattcc tgctcgcgcg ccaaggcccc
    12541 ggccggtggt ccggaattcg tgcttctcga tcttcagggc gacgtttgtg ccctcgcaac
    12601 gtacgacgcg gccgcaagcc accagtttca ggccgcactt cccgtcgagt tggaccggcc
    12661 aactgatgga aagctcgatc cgcttgcccg gaggcaaagg tgcttgcgcg ttgaacaaca
    12721 ctccggtgct gctgatgttg atcgtctcgc cggtaccgat ttcgacgacg cccttcgaat
    12781 tcctcacctt gtaacgcact tcacggacga tcggaaacca atccggcggt gccgccggcg
    12841 cctgcattac gtctgctgac gacgccgtac ggcgctgctc ggcgcgggga ccgaacgcga
    12901 tcccagttct gacgcaattc atctgagcct ctcttcaatc agattttccc tcttgaactg
    12961 cgaactcgca ccagtacaca cgcaatgtct tgtcttgaac cacgatcaac gcaatgacga
    13021 gatttacgaa aggtatgttc ttgcactcct caagacctaa gcaaaggcaa ttcgctgacc
    13081 aatcgccgga agcgacccga gacaaggaaa gtggcgggct cgccaggacc cagcagaagc
    13141 aagtgtgctg gcgccgcccg cgagtccata gtccttctgg cttgcctgaa ggcgctcaca
    13201 cacacgccga aggcagtgcg tctccgcctc ctgaacgggc tgtgcgcggt ggtcagccgt
    13261 caggcaatcc tacgtccctg aatgatttgg agtaccacca cgaccaatgc gacgaccagt
    13321 aagaggtgca gcaggccacc cattgtgtaa gaggtgacca aacccagcac ccacaacacg
    13381 aagagaacta cagcaagtgt ccaaagcatg ttcttacgct cctttacacg aatcgttcac
    13441 tgcacgaatt ggtccatacg ggaatctgcg ccacttcgag ggctgcagcc cccgattcag
    13501 gggtggccag ccggcaatcc gcctgctgca agatgcagca cctgcactcg aatgctcaat
    13561 ttgctgcacg tcgctcgttc aagccccctc tgagaagttc agatggattt cgtgcgccga
    13621 ctgccaaccg ggcggacatc gcaacgcggg cagttcctcc aactggcgat cgccgagccg
    13681 ggtggtttca cgagagtgcg tcaacgagat attctgttgc ttcgtactta tcgcggtcgg
    13741 cagcctcctg gcactcgata caaagcggcg cccatggcaa ggcagcgagg cgttttgggc
    13801 tgatcgccca tgcgcactca atgcaggttc caaaggtgcc gtcgtgaatc cgtcgcaatg
    13861 cggcttttac gtcacgcagc agggtggact ggctgtccac gtttcgaatc gccagatctc
    13921 gttccgacgc gtactgaatc tcatccattt ggtccgcgct cttctcgatt gcgataccgt
    13981 cccgctttcg taatacctga accagttcag cctcttttcg ttcaaggatc ttttggaacc
    14041 gacgcatctc gctgtgtgct atcttcatgg ccatcccctt cgcccgtgcg ggcagcctat
    14101 cgtcctatgc acgagatggt ccagaacaga accccgctat ttcggaggct aagcccgctg
    14161 ggtcagggtg gcggaccggg aattcacccg ggccgaggtg caaaggctgc acctgaaatg
    14221 cgaaatttgc cgacccgcga tctgcgaatg ccggttctac agtaatccgt tctcgcgctc
    14281 agccgcgaaa cgaacagcgg tcacggctgc ccagcggtac tggcatccga atccagggtc
    14341 tcgcgcactc tttgcgcgag tccaacggtt gtaaaaggct tctgcaaaaa cgcggttccc
    14401 ttctggacgc cctccttgag gacgacatcc tgcgtgtgcc cggacatgaa catgactttc
    14461 atgccgggcc gtagcttgag ggcggcttcc acgagttcgc gtccaccgag ttccggcatg
    14521 accacatcgg acacaagcag atcgattggg ccctcgtggg tttcgcacag cgccagacct
    14581 tcccgtccgt tgcgggcgtc gagaaccacg tacccgctcg cctccagaat ttttcgtgcg
    14641 agcacgcgga ccatatcttc gtcttccacc agcaaaatga cttcggagcc cttcggcgcc
    14701 tcggcaaggc ctccagccgg ccgcactccc ttgtccaccg cctcggcgac tgccggcagg
    14761 aagatggtga agaccgtccc ccggcctggt tcggactcgc aacgaatagc gccaccgctt
    14821 tgctcgacga ttcccagaac ggtggccagc cccaggccgg ttcctttgcc ggtctctttc
    14881 gtcgtgaaga acggttcgaa gatgtgggcc tgcatttccg cgttcatgcc gacgccggta
    14941 tctcgcacgg ccatcgtcac gtagcgacct ggcgcaaggt ctggctcgct ggcatcggcc
    15001 tccgggaggg tcacgttggc cgtttcaatg gccagcgtgc cgccgtccgg catggcgtct
    15061 ctggcgttca gggaaaggtt catgatcgcg cggccgattt cgccggggtc ggccctcact
    15121 tgccagaggg cgggcgcgca attgatagcc accctgatcc gttcgcccac caaacggcgc
    15181 agcattcggt cgaactccgc cacaatgaca ttcaggtcga gaaccttggg ctgcagcacc
    15241 tgccgccggc tgaaggccag caactgttga gtgagtaaag ccgcccggtc gccggctttc
    15301 ttgatttcaa gcacctgctg gatagccggc tcgttgccgg ccagcgtatc ggcaaggagc
    15361 tcgctgtagc cgatgatggc cgtgagcagg ttgttgaagt cgtgagcgat gccgccggcg
    15421 agcctgccga cggcctccat tttctgtgat tggcggagat gctcctcgct cctgcgtagg
    15481 gcctgctggg ccagtttgcg ctcggtaatg tcgtcaactg ccagcagaat catcttgaga
    15541 tgattgatgc ggcagccccc gagcaccagg ctcctgcgcc ccacggttgg aaaatgctgc
    15601 tcgacttcga aatccgggaa cgagttgccg ccctggagaa gaccgtccag cgaatcccgc
    15661 atgccgggaa ggtcccaact gccgcgcgac agcgaataga tgacctgtcc ctcggcttcc
    15721 agcggtgacg attgaaacgt ccggtaaaag gctcgattcg ccatcctgat ccgcaaatcc
    15781 ggatcgagca ccaacaacgg ctggcggacg gtttctacaa tcgacatcgc gaagtcgcgc
    15841 gcctgcccga gcgccgcgtt tttggcctgc aattcatcgt tgaccgtgat cagctcctcg
    15901 ttggtggatt gcagctcttc cttggcggtc tccagctcct cgttgaggct ttgcagttcc
    15961 tcgttggccg aaagcgcctc ctctgtcgtg ttctggcttt cttccctgga taactggtgc
    16021 tcttccatcg ccgaaagaaa cctctgtttc gcgtcggcga gttgctgttt cagcctggag
    16081 atctggcggt cccgggcatc ggcttcggga ggggcgtccg gcggttcggt ctcgcgtccg
    16141 gcggcgcccg gcacgggttc gaaaaggaca aggatcgacc gcttttgact ggcgtccaac
    16201 ggcaccgcct ccacgttcaa ctcgccggcg ctcccgtcgt gttcgtaccg tactcgctcc
    16261 tgccgcgccg gctcgccgct gtgccgcacc tgttggatca acttttcgac atccaggaat
    16321 agtccggtgt ctgggatcag cttcataaga ttgaagctca actttccaac cggcagcgtt
    16381 aggtaggggc tggccttgcc tcgaatctca gtgacctcca ggtccccgtc caccaccact
    16441 ccggccgggc tgtacttcga aaggagtatg cgatcgacct ctttccgcac gtccgcgccg
    16501 ccccacaatt ccggagccgg cgccgcggag gcgctgcccg ccggcgcccc ccggcgtgaa
    16561 ccacgggcgg ccgcgtgaaa caagtgcggt ttcctcgccg tttcccgctt ggcatagatt
    16621 cggtgttcgc ggtcggccac cgaaaacagg tcgccagacg ccgccgcctc cgatgcgccc
    16681 aacatgagaa agccggtcgg cttgagcgcg tagtggaaca gcgggatgat gtccttctgt
    16741 acacttccca agtagatcag cacgttgcgg caactgatca ggtccagctt cgaaaacggc
    16801 ggatcgtcga tcaggttatg cctggtaaag acgcacatct cgcgcaggtc cctattgatt
    16861 tggtagccgc cttcgatctt tgtgaaatag cggttgaggc gctcatgggt aaggtctgcc
    16921 gcaatgttct cgagatacct gccggtgcgt gccttttcaa ttgccgccag gctgacatcg
    16981 gaagcaaaga tctgtaccgg aaaggtggcg cccgtttcgt tcaggtgttc ctgtaacgtt
    17041 atggcaatcg agaatgcctc ttctcccgtg gcgcaacccg ccacccagac gcgaatcgtc
    17101 tcgttcgcgg aacggccttg caggatgagg ggaaagacag ctttcttcag gcgctcgaac
    17161 gattcccgat cgcggaaaaa gctggtcacg ccgatgagca aatcccgctg gagagccatc
    17221 agctcaccgg aatcattctc cagacgcttt ccgtattccg cgagcccgtc gatattgcgc
    17281 agggcaaggc gccgcaggat gcggcgtttg atcatctttt cccggta
//
LOCUS       DATIFQ010000538         7971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1718240, whole genome shotgun sequence.
ACCESSION   DATIFQ010000538 DATIFQ010000000
VERSION     DATIFQ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718240"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VLE22_27120"
     CDS             <1..209
                     /locus_tag="VLE22_27120"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HSB18154.1"
                     /translation="VPAGSVAIGGTQTGIYPLPSPGGWRIIGRTSLRLFEPRRRPLTL
                     IEMGDHVRFRPISAREFKELESRS"
     gene            212..1147
                     /locus_tag="VLE22_27125"
     CDS             212..1147
                     /locus_tag="VLE22_27125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HSB18155.1"
                     /translation="MPGLRVVEPGFLSSVQDLGRFGFAHLGISPCGAADTFSLRAGNR
                     LTGNLDSAAAIEMTLTGGTFIFEQETVFAITGSDFGASVGGRPVTCWAATHAEAGAQL
                     RLGGARDGARCYLCVSGGIVVDDFLGSASTDLRANFGGLEGRPLRKGDRLEIGQRANP
                     MPGVDARPLRYYVRHSTLRVTRGPQARLFPSDALGRLCDGAYTVSNDSSRLGVRLFGG
                     SIRASSRVQLLTEGVSLGAVQVPPDGQPIILFVDQQTTGGYPKLANVILADLPRVGQL
                     APRSEVRFVEVDVATAHAALREREARLTAVLRGEA"
     gene            1144..2364
                     /locus_tag="VLE22_27130"
     CDS             1144..2364
                     /locus_tag="VLE22_27130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB18156.1"
                     /translation="MSSKAAWWRDITGSQWRTLVAALVGWMLDAMDMMLFTFALNPIR
                     AEFQLTGSGVGSVAAAALLTSAAGGILFGFLADRYGRARALSWSILIYSFATALTATS
                     RSLEELLIWRMLLGIGMGGEWSAGAVLVAETWPAEHRGKAIGIMQSGWAAGYLLAALL
                     SAAILPSYGWRWLFLLGALPALLAVWIRRQVEEPKGWRVSKKGGGGISLSLVAVRGRI
                     LRASLLTTCVLFAYWGLFTWLPAYLGTARELGGAGLSIVKTSAWMVPVQIGALFGYLS
                     FGFLADRFGRRPVFITFVLAAAVLVPVYGWSARWVGVLLVLGPLIGFFGHGYFSVFGA
                     MLAELFPVRIRGTAQGLSYNVGRAASALAPVAIGMLADRFGIGPALALTSAFFIAGAG
                     LILCLPETKAVSLS"
     gene            2376..3104
                     /gene="pxpA"
                     /locus_tag="VLE22_27135"
     CDS             2376..3104
                     /gene="pxpA"
                     /locus_tag="VLE22_27135"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HSB18157.1"
                     /translation="MRLWLNSDVGERPEALLDGSEEELIRLLDWANIACGGHAGDEAS
                     MAHVVRLCRKHCVQIGAHPSYPDRERFGRAVVDLPGQVISQFVFEQVKSLDTVARAQG
                     EQVRHIKPHGALYNRAARDRAVALAIGEGVGRWNRKAVLVGLAGSGMLDVWREQGFSI
                     AAEAFADRRYEPDGSLRARSFPDALITDPAEAVAQALLVSGGRVRAWDGSEVAVSAGT
                     ICIHSDTPEALAIARAVDGVLRHA"
     gene            3396..7028
                     /locus_tag="VLE22_27140"
     CDS             3396..7028
                     /locus_tag="VLE22_27140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB18158.1"
                     /translation="MNCSLQMLRKSLLLLVIASIPLAAQTGNGIVRGTVLDATKAAIP
                     SAKVTLTNTATGIARGAQSSAVGIYYFGAVPVGPYTLAVEAQGFKKWSGTLTVEVGQV
                     VVIDPSLEVGALEATVEVTGAAPVLTTEGMQVADVKDALRIQMLPLNGRVITNLFNLT
                     PGVEGGGNPRVNGMKVGSAEMLLDGISLVDRFGGGISRVTPSLDTIQEYRVETAGSGA
                     QYSRPTTFTMVSKSGTNEIHGAAFWTHRNNFGGLRARQRQDTAVDPVTGKFVQSQYIR
                     NEYGVSAGGPVIKNKTFWFFSYEGQKERQGRFARTQAPTDAIWNGDFSTMTDYDYNPI
                     TIYNPLTTNAQGVRTPFEGNKIPSNLIQPIAATMRSISAAPTDLTADPWTEPNFTAYY
                     PNKADVTMYTAKGDHVFSEKDNVSGRFTRSVRPRKILGGVYGYPPPGTTDSGGSSRSD
                     ASMNSMYARWNHVFSPTFMNEFQASAHRSSNSSGTTGDNTNWADKLGFPNPFGVTGWP
                     TICTDAPFYYWGCWDGDNRGDQQLTQFQLEDNTTWIKGKHTVKVGFKGRQEYNNVREL
                     QQAQGSHSFYGSWTALYDPAEEQGVSYTGSGFAGMLMGLPTYLSNQYNRGYFYFQQKE
                     IGLYFQDTWKVSSRLTLDLGLRFDKWTVYHEKYNRLVNLDITDYAGKMEVFTPHDTKM
                     ESIPGIPKSVLASWAARGLSWKTAKEVGFPASLLPADNNNFAPRLGAAYRFSEKWVLR
                     AGFGMYYWPMPLSQILQSSRTNPPLNLRFENQIDNLNGKNWVYGLSSVPAATDYIGKA
                     TVNTEGIVELPSSAQGMMPWDIHAWSDNQMREWTFTIERELMKETALRLSYIGNHGSN
                     LEQRWRWNDAESQWNYQARTGLQAQTNADLRRVNPNWDSGCCQAPIRHNGFSNNHSFQ
                     AEVERRYSNGLAFQWFYTISHAMTTNDAGGFTYGSSRINSSGSGTIYAVPENRLIIGE
                     PNLSEDQRLRFGYANSDAVPAHRIRWNGIYDLPFGKGKKYAGNVSSALNHVVGGWQIA
                     FIGEWRGGNWMSVDSGRYLFGDPTLTADERLTMTYAGRTQRLWFKGDMNPTQASGVDQ
                     AKLTALVPVDRSQRVLRPVGDKFDNRIPQMLANGTVRYTGVNDMLNWNARNFFRGPGA
                     WNQDVSVFKYFSFTEKTKLRFTADFFNATNSPMDNNPSSGTGLQDLSTQPNDPRIIQL
                     TLRLEW"
     gene            complement(7162..7599)
                     /locus_tag="VLE22_27145"
     CDS             complement(7162..7599)
                     /locus_tag="VLE22_27145"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HSB18159.1"
                     /translation="MKLFTIGFTKKSAESFFTSLKAAGVRRLVDVRLNNISQLAGFTK
                     RDDLRYFTRAICGIDYTHIPLLAPTQDILDAFKKRKGDWRLYERQFLELMRTRQVENE
                     VSRELLDGACLLCSEETPLHCHRRLIAEYLKEKWGRVEIEHLS"
BASE COUNT         1531 a         2307 c         2519 g         1614 t
ORIGIN      
        1 tggttccggc gggcagcgta gccatcggcg gaacccagac cggaatatat ccgctcccat
       61 ccccgggagg ttggcggatt ataggccgta cttcgttacg gttgttcgag ccacgccggc
      121 ggccgctgac gctgatcgag atgggggatc acgttcgttt tcgtcctatt tccgcgcgtg
      181 agttcaaaga actggagagc cggtcgtaac tatgccaggg ttgcgcgtgg tagaacccgg
      241 cttcctctca agcgttcagg accttggccg atttggcttt gctcaccttg gtatttctcc
      301 gtgcggcgcc gccgatacat tttcgttgcg agcggggaac cgattgactg gcaatcttga
      361 ttccgccgca gcaatcgaaa tgacgctcac cggagggaca ttcattttcg agcaggagac
      421 ggtattcgct atcaccggtt cagatttcgg agcgtccgtg ggtggccggc ccgtgacttg
      481 ctgggcggcg acgcacgcgg aagcaggcgc gcaacttcgc cttggcggcg cgcgcgacgg
      541 cgcgcgctgc tacctttgcg taagcggtgg aattgtggtg gacgatttcc tgggcagcgc
      601 gtcaaccgat ctccgcgcca atttcggagg ccttgagggg cgtccgttga ggaaaggaga
      661 tcggctggag atcggacaga gagcgaatcc catgcctggc gtcgatgcac gaccgctccg
      721 gtactatgtc aggcacagca cgttgcgagt cacacggggg cctcaagcac gtttattccc
      781 ctcggacgca ctaggacgcc tttgcgatgg cgcttacacc gtgagtaatg actccagccg
      841 cctcggtgtc cgcctttttg gtggatcgat acgggcttca tcccgggtgc agctcctgac
      901 cgaaggagtc tcgctgggtg cggttcaagt gccgcctgac gggcagccaa tcatcctatt
      961 tgtggaccag cagacgacgg gcggataccc gaagctcgcc aacgtgatcc tggcggatct
     1021 gccgcgggtg gggcagttag ctccgcgaag tgaggtgcga tttgtggaag tcgatgtcgc
     1081 cacggctcac gcggcgctgc gcgagcgcga agcccgccta acagccgtgt tgcgtggtga
     1141 agcgtgagca gcaaggccgc gtggtggcgg gacatcaccg gaagccagtg gcgcactcta
     1201 gtagctgcgc tcgtgggttg gatgttggac gcgatggata tgatgttgtt cacattcgcc
     1261 ctaaacccaa ttcgcgctga atttcaattg accgggagcg gagttggcag cgttgctgcg
     1321 gcggcattac tgacatcggc cgcagggggc atactattcg ggtttcttgc cgaccgctac
     1381 gggcgtgctc gcgcattgag ctggtcgatc ctgatctact cgtttgccac ggcgctgacg
     1441 gcgacctcac gctcgcttga ggagctactg atttggcgaa tgttgcttgg tatagggatg
     1501 gggggcgagt ggtcggcagg agcggtcctc gttgcagaaa cctggccggc ggagcatagg
     1561 ggcaaagcga tcgggatcat gcagtcgggg tgggcggcag gatacctgct ggcggctctg
     1621 ctgtcggcgg ccatcctgcc ctcgtatgga tggcggtggt tgttcctgct gggcgcactg
     1681 cccgccctgt tggcagtctg gatacggcgc caagtagagg agcccaaagg ctggcgcgtg
     1741 tcgaagaaag gtggtggcgg gatttccttg tcgctcgttg cggtacgagg tcgtatccta
     1801 cgtgcttcgc tgctgaccac ctgtgttctc ttcgcgtatt ggggactgtt cacgtggctg
     1861 ccggcttatc tgggcacagc gcgggagcta ggaggggccg gcctgagcat cgtaaagaca
     1921 tcggcctgga tggtcccggt tcagattggg gcgttgtttg ggtacctgag tttcggcttt
     1981 ctagcggacc gcttcgggcg ccggccggtt ttcatcactt tcgtgttggc ggcggctgtg
     2041 cttgtgccgg tttatggctg gtcggcgcgg tgggttggtg ttctgctggt gctagggcct
     2101 ctgattggct tctttgggca cggatacttc agcgtgtttg gggcgatgct tgccgaacta
     2161 ttcccagtaa ggatccgtgg cacggcgcaa ggtctctctt acaacgtggg gagagcggcg
     2221 agcgcactgg cgccggttgc gatcgggatg ctcgccgacc gctttgggat cgggccagcg
     2281 ctagcgttga cttcggcctt ctttattgcc ggcgcgggac tgattctgtg tctccctgaa
     2341 accaaagcgg tctccctttc ataatggtgg ggaaaatgcg gctatggctg aattccgacg
     2401 tgggtgaacg gcccgaggcc ttgctggacg ggagcgaaga ggagttgata cgtctgctgg
     2461 attgggcgaa cattgcctgt ggcggccacg ccggcgatga ggcatcaatg gcgcacgtcg
     2521 tgcggttgtg ccggaagcac tgcgtgcaga taggtgcaca cccgagctac ccggatcgag
     2581 aacgtttcgg ccgcgccgtt gtcgacctgc ccgggcaagt gatttcgcag ttcgtgtttg
     2641 agcaggtcaa atcgctggac accgtcgcac gggcccaggg cgagcaagtg cggcacatca
     2701 aaccgcatgg ggcactgtac aaccgggcgg ccagggatcg ggcggtggcg cttgcgattg
     2761 gtgagggcgt gggccgctgg aaccggaagg cggtgctggt cggcctagca ggctccggca
     2821 tgctggatgt ctggcgggaa cagggctttt ccattgcggc cgaggctttt gcagaccgcc
     2881 gttacgagcc ggacggttcg ttgcgggcac gatcctttcc cgatgcgctt ataacggacc
     2941 cggcagaagc cgtcgcgcaa gcgctgctgg tctccggggg ccgggttcgc gcctgggacg
     3001 gcagcgaggt tgcggtaagc gcggggacga tctgtatcca cagcgacacg ccggaggcgc
     3061 tggcaatcgc gcgagcagtt gatggagtgc tgcggcacgc atagagccgt cgcagcatcg
     3121 ttctcgcggc gccgcctcgg catggtctgc acgccggcca acttcgccct gtttccgtgc
     3181 acgctgggat acctgctgct ctacgccgga tgggtccacg cgcggcaggt gagaatggcg
     3241 atgcgaggcg ggtaaagcga agcctgacgg aaccaaaaaa gaataatatt taacgttcca
     3301 cggtgacggg cacggttccc cacctcttga catcggcccc gtgcgttgac tacgattctc
     3361 gacgcattct cgacacaggc agcttggagg ttggaatgaa ctgttccttg caaatgctaa
     3421 gaaagtctct cctcctgctg gttattgcca gcatcccatt ggcggcccag accggcaacg
     3481 gcatcgttcg aggtaccgtg ctggacgcga caaaggccgc cattccatcg gcgaaagtaa
     3541 ccctgacgaa tacagcgacg ggcatagcgc gcggcgcaca atcgtcggct gtcggtatct
     3601 actactttgg cgcggttccc gtcggcccgt ataccttggc cgtcgaggcc cagggcttca
     3661 agaaatggtc gggcacactc acggtcgagg tcggccaggt ggtcgtgatc gacccatccc
     3721 tggaggtcgg cgcgcttgag gccacggtcg aggtcacggg cgccgctccc gtcctgacga
     3781 ccgagggcat gcaggttgca gacgtcaaag acgccttgcg catccagatg ttgccgctca
     3841 acgggcgcgt gatcaccaac ctgttcaacc tcacgcccgg cgtcgagggc ggcggcaacc
     3901 cccgggtcaa cggcatgaag gtcgggtccg ctgagatgtt gctcgatgga atttcgctgg
     3961 ttgaccgctt cggcggcggc atctcgcgtg tgaccccgag cctcgatacc attcaggagt
     4021 accgcgtgga gaccgccggt tcgggagcgc agtactcccg tccgacgaca ttcacgatgg
     4081 taagcaagag cggaaccaac gaaatccacg gcgcggcttt ctggacgcac cgcaacaact
     4141 ttggcggcct tcgtgcccgc cagaggcagg acactgccgt cgatccggtg acaggaaagt
     4201 tcgtgcagag ccagtacatc cgcaacgaat acggtgtctc ggccggcggg ccggtcatca
     4261 agaacaagac gttctggttc ttctcctatg aaggccagaa ggaacgccag ggccgttttg
     4321 cgcggaccca ggctcccact gacgccatct ggaacggcga cttcagcacg atgacggact
     4381 acgactacaa cccgatcact atctataacc cactgacgac caacgcccaa ggcgtccgga
     4441 cgccgtttga gggcaacaag atcccgtcca acctgatcca gccgatcgcc gcgacaatgc
     4501 gcagtatttc ggcggcgccc accgatctga cggcggaccc gtggaccgaa ccgaacttca
     4561 cggcttatta cccgaacaag gccgacgtga cgatgtatac ggccaagggt gaccacgtct
     4621 tttccgagaa ggacaacgtc tccgggcggt tcacgaggtc ggtgcggccg agaaagatcc
     4681 tgggtggcgt gtacgggtat cctcctccgg gcacgacgga ttccggcggg agcagccgtt
     4741 cggacgcaag catgaactcc atgtatgcgc gctggaacca tgtgttctcg ccgacgttca
     4801 tgaacgagtt tcaggcctcc gcgcaccgct cctcgaatag ctcgggtacg acgggggaca
     4861 acacgaactg ggcggacaag ctagggttcc ccaacccgtt tggcgttacc gggtggccca
     4921 ccatctgcac ggatgccccg ttctactatt ggggctgctg ggacggagac aatcgcggcg
     4981 accagcagtt gacccaattc cagctggagg ataataccac ctggatcaag gggaagcaca
     5041 ccgttaaggt tggtttcaag ggacggcagg aatacaacaa cgtgcgcgag cttcagcagg
     5101 cccagggctc ccattccttc tacggttcct ggacggcgtt gtacgacccc gcagaggagc
     5161 agggcgtttc ttacacaggc agcggtttcg ccggcatgtt gatgggtctg cctacgtatc
     5221 tttcgaacca gtacaaccgc ggatatttct atttccagca gaaggagatt ggcctctact
     5281 tccaggacac atggaaggta agttcacggc tgactctgga cctgggactc cgtttcgaca
     5341 agtggacagt ctaccacgag aaatacaacc gtcttgtcaa cctggacatc accgactacg
     5401 ccggcaagat ggaggtcttc acgccgcacg acaccaagat ggaatcgatt ccaggtattc
     5461 caaagtcggt tctggcatca tgggccgctc gggggctgtc ctggaaaacc gcgaaggaag
     5521 ttggtttccc ggcgagctta ctgccggccg acaacaacaa cttcgccccg cgtcttggcg
     5581 cggcctaccg tttctccgaa aagtgggtct tgcgggcggg tttcggaatg tattactggc
     5641 cgatgccgtt gtcgcagatc cttcagagct cgcgcacaaa tccaccgctg aacctccggt
     5701 ttgagaacca gattgacaac ctgaacggca agaactgggt ctacggactg tcatccgtcc
     5761 cggccgcgac tgactacatt ggaaaggcga ccgtcaatac ggaaggcatc gtggaactac
     5821 cctcttcggc acagggcatg atgccctggg acatccacgc atggagcgac aaccagatgc
     5881 gggagtggac ctttaccatc gagcgggagt tgatgaagga aaccgccctc cggttgagct
     5941 acatcggcaa ccacggaagc aacctcgaac aacgctggcg ttggaacgac gccgaatccc
     6001 agtggaacta ccaggcccgc acggggttgc aggcacagac caacgcggat ctgcgccggg
     6061 tcaacccgaa ctgggacagt ggctgttgcc aggcgccgat ccgtcacaac ggcttctcca
     6121 acaaccattc gttccaggcg gaggtcgagc ggcgatactc gaacggcctc gccttccagt
     6181 ggttctacac gatctcgcac gctatgacca ccaatgatgc cggtggcttc acgtacgggt
     6241 ccagcaggat caactcgagc ggcagcggca cgatctacgc cgtgcccgaa aaccgcctga
     6301 tcatcggcga accgaacctc agcgaagatc agcgcttgcg cttcggctac gcgaactccg
     6361 acgccgtacc ggcccaccgg atccggtgga acggtattta cgacctccca ttcggtaagg
     6421 ggaagaagta cgccggcaat gtttccagcg ctctgaatca cgttgtgggc ggctggcaga
     6481 tcgcgttcat cggcgagtgg cgcggcggca actggatgag cgtggattcc ggcaggtact
     6541 tgttcggcga tccgacgctc acggcggacg agcgcctgac gatgacctat gccgggcgca
     6601 cccagcgcct ttggttcaag ggcgatatga acccgactca agcctccggc gtcgatcagg
     6661 cgaagttgac ggcgcttgtt ccggtggatc gctcgcagcg agtgttgaga ccggtgggcg
     6721 acaagtttga caacagaatt ccgcagatgc ttgcgaacgg caccgtccgc tacaccggcg
     6781 tcaacgacat gttgaactgg aacgcgcgga acttcttccg cggtcccgga gcatggaatc
     6841 aggatgtctc cgtgttcaag tacttctcct tcaccgagaa gacgaagctt cgcttcacgg
     6901 cggatttttt caacgcaacc aacagtccga tggacaacaa tcccagctcg ggaactggat
     6961 tgcaggacct cagcacccag cccaacgacc cgcgcatcat tcagttgaca ctccgtctgg
     7021 agtggtaacc ggctggcttt tcctaactcg gggcacggtt ccggccgtgc cccagtaagc
     7081 gcggttccct cttcggaggc cgtcgggacg gctacgccct attcgtgatt tgatggcgaa
     7141 gtggtagtca gtccccattt cttaagagag atgttcaatt tcgacgcggc cccacttttc
     7201 tttgaggtac tcggcgatca gccggcgatg acagtgaagc ggcgtttcct cgctacagag
     7261 cagacaggct ccgtccaaca attcgcgtga gacttcattc tcgacctggc gcgtcctcat
     7321 cagttcaagg aactggcgct cgtacagcct ccagtcgcct ttccgctttt tgaaggcatc
     7381 gaggatatct tgcgtcggag caagaagagg aatgtgcgtg tagtcgatcc cgcaaatcgc
     7441 cctggtgaag taacgcaggt catctcgctt ggtgaagccc gcgagttgcg agatgttgtt
     7501 gagccgaaca tccacgagcc gcctgacacc cgcagctttc aagcttgtga aaaacgactc
     7561 ggcggacttt ttcgtgaaac cgatcgtgaa gagcttcatc ccatagcaat cttagtattt
     7621 cactgggact ccgggactag cgtatcacct cggcctcaac gctgggatac gtctgcgggg
     7681 cgggggtaag agccgcaaaa gcagcactgg aagcccggaa atgcgtctac agttccggtt
     7741 ttccgtacta gtatcgatgg tactggcgac ggcagttgtc tattgcactt tttaaatgca
     7801 gggctcaaaa tgctggaagc gaaatagttc cctcctgggc gaggagatcc aatgaccagc
     7861 aaagcccgtg tccttccttt tgctctggcc gcaatctcgg tgcaactcgc tgggcaggca
     7921 ccggagcgag tcagtgttct ggtgcacctt gcgccccgcg ccggccgcgc t
//
LOCUS       DATIFQ010000539         4794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1912419, whole genome shotgun sequence.
ACCESSION   DATIFQ010000539 DATIFQ010000000
VERSION     DATIFQ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912419"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VLE22_27150"
     CDS             complement(<1..1112)
                     /locus_tag="VLE22_27150"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18160.1"
                     /translation="MEDFILIRGARVHNLKNVSLAIPHNLLTVVTGVSGSGKSSLVFD
                     TIYAEGQRRYVESLSAYARQFLERMEKPDVDEIAGLAPPIAIRQKNTTRNPRSTVATA
                     TELYDFLRLLYARAGRTYCPNCGRQVEKDTVDQVAGRMQAEPSGSRWYALFPIRQEPA
                     GDTRALRDHLFELRKKGFNRLFQHGRMFEFSTPESLLEIDFAEPLFALVDRIVMAPDL
                     HQRLVDTTETCYREAGEIIFERAGVEPPERLRFSEKFACKTCGIEFAEPEPRLFSFNS
                     PTGACPRCQGFGNTIDYDMDRVIPDRSLSLEEGAVEPWTKPQHRWGMWELKRHGRGKV
                     RLNVPFADLTDAEKRFLIEGEGRFRGIRGFFEKLET"
     gene            1161..2138
                     /gene="mutY"
                     /locus_tag="VLE22_27155"
     CDS             1161..2138
                     /gene="mutY"
                     /locus_tag="VLE22_27155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HSB18161.1"
                     /translation="MKLFRRLLLRWYDRAARPLPWRTNAGPYRIWVSEIMLQQTRVAA
                     VLPYYERFLARFPDAASLAAAPESELLALWSGLGYYSRARNMQSAARKIVELGSFPRD
                     YDQIRRLPGVGDYTAAAIGSIAFGLPYAVLDGNVLRVLSRLTNDAGDIASTVVRRRLH
                     EKAQALLDPGRPGEFNQALMELGAIICLPRAPQCLLCPIAELCEARRLGVENQLPVKL
                     GRVKQVRVHKTLLLAERNGRVLLWQRSCGGRMNGFWELPEAGQLPEARHLETLHEFRH
                     SITNHSYVFTVVSVRVRGNPEGFSWIKRALLNEIPLSTTARKALARSEV"
     gene            2216..3541
                     /locus_tag="VLE22_27160"
     CDS             2216..3541
                     /locus_tag="VLE22_27160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HSB18162.1"
                     /translation="MKHPRIILFGKFAAILSVVPALFYGYSSGPPAERTGAPGEQTCF
                     ASECHSGTFLQSSGNINVDFGASATYTPGGPKQVWHVNSSDSLAVIFGFQLSVRKESD
                     NGQAGTLGVPSGDTSVRVIQSAAGIQYIEHTGPKPPAAMPFTFEWTPPATDVGNIKVY
                     LAMNAANGNFLPTGDRIHTRSFTLTPAVAKPKPTISQGGVVNAWSSKPLVSDSAWISI
                     YGSNFTTNTRTWKSEEIVNGKFPTQLDGVGVKINNQDAFVYFVSPGQINVQIPSDGAA
                     GTMSVQVINPDGVSEAQLVNKQAVGPALFTWAGVVPGGGTYVGALFPDFKYAGKLGLL
                     QPVGIETRPAKPGEFVLLFATGCGPTNPAVPAGEVVGQPTPTLTSPVTVRVNGTPAEV
                     FNNTGFLIFAGECQFNVKIPDNAPDGDLPVELEIGGVRTDQDVRITVQK"
     gene            3624..4601
                     /locus_tag="VLE22_27165"
     CDS             3624..4601
                     /locus_tag="VLE22_27165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18163.1"
                     /translation="MGKWWPAVLLLTLAVLASADCPPQAPCYTEAGIVNGASFVAGSL
                     APNTLASIYGSDLAWTPRPLAAEDILGGRLPVRLGGVTVHVGAYQAPLYYVSPNQVNV
                     LIPANLRPGEHVIRVVRDGWAGPAARIRLSDAAPALFLLDEHTPVATRPDFSVITPDS
                     PAAAGEYVILWATGLGETVPYVGSYDELPLSAAPLARRNEFQVLVDGQAVDSSRIDYA
                     GVAPGFGGLYQINFRLPETLSANPEVRIALRTSFSPSGIRLPAGGYSLNFSSRAVRSR
                     PVGTVSGPYNSGRRAQIHIRPVLWGSREASRSAITMSSPALFVSARTRP"
BASE COUNT          879 a         1578 c         1444 g          893 t
ORIGIN      
        1 ttagtttcga gtttttcgaa gaaaccgcgg attccgcgga aacgcccttc gccttcgatc
       61 aagaaacgtt tctcggcgtc cgtcagatcg gcgaaaggca cgttcaaccg caccttgccg
      121 cggccgtggc gcttcagctc ccacatgccc cagcgatgct gcggcttcgt ccacggctcc
      181 accgcgcctt cttcgagaga cagagatctg tccggaatga cccggtccat gtcgtagtcg
      241 atcgtgttgc cgaatccctg gcagcgggga cacgcgccgg tcgggctgtt gaagctgaac
      301 agccgcggct cgggctcggc gaactcgatg ccgcacgtct tgcaggcgaa cttctcgctg
      361 aagcgcaggc gctcgggggg ctccacgccg gcgcgctcga agatgatctc tcccgcctcg
      421 cggtaacacg tttccgtggt gtccactagc cgttggtgaa ggtccggcgc catcacaatg
      481 cggtcgacga gcgcgaacag cggctcggcg aaatcgatct ccagcagcga ctccggggtt
      541 gagaactcga acatccggcc gtgctggaac aggcggttga agcctttttt ccgcaactcg
      601 aagaggtgat cgcgcagcgc cctcgtgtcg cccgccggct cctggcggat agggaacagg
      661 gcgtaccacc gcgagccaga cggctccgcc tgcatcctcc cggcgacctg atccaccgtg
      721 tccttctcaa cctgccgccc gcaattcggg cagtatgtgc gaccggctcg ggcgtagagc
      781 aggcgcagga agtcgtacaa ctcagtcgcc gtcgctaccg tcgatcgagg gttgcgggtg
      841 gtattcttct gccggatcgc gatcggcggc gccaggcccg cgatctcatc cacgtcgggc
      901 ttctccatcc gttcgagaaa ttgccgcgca taagccgaca gcgattcgac ataacgtcgc
      961 tgaccctcgg catagatcgt gtcgaagaca agggaggatt ttcccgagcc ggacacgccg
     1021 gtcactaccg tgagcaggtt gtgcggaatc gcgagcgaga cgtttttcag gttgtggacc
     1081 cgcgccccgc gaatcaggat gaaatcttcc actacaattt attgtattct ccgagttcgc
     1141 cgcgcctcag cgctctatcc atgaaactgt tccgccggct ccttctccgc tggtatgacc
     1201 gtgccgcccg gccgctgccg tggcgcacga acgcgggacc ataccgaatc tgggtctcgg
     1261 agatcatgct gcagcagacg cgcgtcgccg cagtcctgcc ctactacgag cgattcctgg
     1321 cgcgcttccc ggacgccgcc agtctcgccg cggctcccga aagcgaattg ctggctctgt
     1381 ggagcggcct cggctactac tcacgcgcga gaaacatgca atcggcggca agaaagatcg
     1441 tcgagctcgg cagctttccg cgcgactacg atcagatccg gcggctcccc ggggtggggg
     1501 attacaccgc cgccgcaatc ggcagtatcg ctttcggact gccgtacgct gtgctcgacg
     1561 gcaacgtctt gcgcgtcctc agccggctca cgaacgacgc gggcgacatc gcgtcaacgg
     1621 tcgttcgacg gcgcttgcac gagaaggcgc aggctttgct cgaccccggg cgcccgggcg
     1681 aattcaacca ggcgctgatg gagcttggcg ctatcatctg cctgccaaga gcaccacagt
     1741 gcctgctttg tccgatcgcg gaactctgtg aagcccgcag gctcggcgtc gagaaccagc
     1801 ttccggtcaa actcggccgc gtaaaacagg tgcgggtgca caaaaccctt ctgctcgcgg
     1861 aaagaaacgg ccgcgttctg ctgtggcaga ggtcctgcgg tgggcgcatg aacggcttct
     1921 gggaactccc ggaggcgggc cagttacccg aagcacggca tctcgagaca ctccacgaat
     1981 tccgccattc catcacgaac cacagctacg tctttacagt cgtctcggtt cgtgtccgcg
     2041 gtaatccgga aggcttttca tggatcaaac gagccctctt gaacgaaatt cccttgagta
     2101 caacggcccg caaagccctc gctcgttcag aggtgtaaaa ccttgtaaac acgtgccttc
     2161 cacgctcgga ttcccatccg ttcccgctta cactgagtag aaagatcaga gggctatgaa
     2221 acatccaagg ataattctat ttggcaagtt cgcggccatt ctttcggtcg ttccggcgtt
     2281 gttctacgga tactccagcg gtccgccggc agaacgcacg ggcgcaccag gcgaacagac
     2341 ctgctttgcc tcagagtgcc acagcgggac gtttctccaa agctcgggaa acatcaatgt
     2401 cgatttcggc gcttcggcga cgtatacgcc tggcggtccg aagcaagttt ggcacgtcaa
     2461 ctcttccgac tcgctggccg ttatcttcgg cttccagttg tcggtccgca aggagagcga
     2521 caacgggcag gcgggcacgc tcggcgtgcc ctcgggggac acctcggtcc gcgtgattca
     2581 gagcgcggcc ggcatccagt acatcgagca tacgggtccg aaaccgccag ccgccatgcc
     2641 gttcaccttc gagtggacgc cgcctgccac ggacgtcggg aacatcaagg tctatcttgc
     2701 aatgaacgcg gccaacggga acttcctacc taccggtgac cgcatacaca ccaggagctt
     2761 caccctcacg ccggcggttg caaagccgaa accgaccatc agccaggggg gcgtcgtgaa
     2821 cgcctggagt tcgaagccgc tcgtctcgga ctccgcgtgg atctcgatct acggcagcaa
     2881 cttcacgaca aatacccgca catggaagag cgaggagatc gtcaacggca aattcccaac
     2941 tcaactcgac ggcgtcggag tgaagatcaa caaccaggac gcgttcgtct acttcgtcag
     3001 ccccggacag atcaacgtcc agatcccgag tgatggcgcg gcgggcacga tgtccgtaca
     3061 agtcatcaac ccggacggtg tgagcgaagc gcagctggtg aataagcaag ccgtcggccc
     3121 ggccctgttc acgtgggcag gagtcgtgcc cggcggtgga acatacgtgg gcgcgctatt
     3181 ccccgacttc aagtacgccg gcaaattggg cctgctccag ccggtcggca tcgaaacgcg
     3241 ccctgccaaa ccgggcgagt tcgtgctgct gtttgcgacc ggctgtggcc cgacgaaccc
     3301 cgccgtaccg gctggcgagg tcgtgggcca gccgacccca acgctgacca gcccggtcac
     3361 cgttcgcgtc aacggcacgc cggccgaggt cttcaacaac accggttttc tgattttcgc
     3421 cggcgaatgc cagttcaacg tgaagattcc ggacaatgct ccggacggcg acctgcccgt
     3481 cgagcttgaa atcggcggcg tccgcactga tcaagacgtt cgaatcaccg tacagaaatg
     3541 acccggagaa ttgtggagag caagccggtg aaacggtaga gaatccaccg ccgccggcgc
     3601 cactatcctc attggaacca gtgatgggga agtggtggcc ggcggttctt cttcttacgc
     3661 tcgccgtgct cgcgagcgcg gactgcccgc cgcaggcgcc ttgctacacc gaagccggca
     3721 tcgtgaacgg cgcgagcttc gtcgccggct ccctcgctcc caataccctt gccagcattt
     3781 acggctccga tctcgcatgg acgccccggc cgctcgcggc cgaagacatt ctcggcgggc
     3841 ggcttccggt ccgtttggga ggcgtcacgg tccacgtcgg cgcctaccag gcacctctgt
     3901 attacgtttc gcccaaccag gtcaacgtgc tgatccccgc caatcttcgg ccgggcgagc
     3961 acgtaatccg cgtggtgagg gacgggtggg cgggcccggc ggcgcggata cgcctctccg
     4021 acgccgcacc ggctctgttt cttttggacg aacacacgcc ggtcgccacg cgcccggatt
     4081 tcagcgtgat cacaccggac tcacccgccg cagccggcga atacgtcatc ctctgggcaa
     4141 ccggcctggg cgagaccgtc ccctatgtcg gctcgtacga tgaattgccc ctgtcggccg
     4201 ccccgctcgc gcgtaggaat gagtttcaag tgctggtcga cggccaggct gtggattcgt
     4261 cccggatcga ctacgccggt gtcgcgcccg gcttcggcgg gctctatcag atcaacttcc
     4321 ggctgccgga aacactctcc gcgaacccgg aggtgcgtat tgcgctgcgg acatcgttca
     4381 gtccttctgg aataagactg cctgccggtg gctattcctt gaatttcagc tctcgcgcgg
     4441 tgcgctcgcg gccggtgggg acggtgagtg gaccgtacaa ctccgggcgg cgggcgcaga
     4501 tccacatccg gccggtgctc tggggcagca gagaggcgtc cagatcggcg atcaccatgt
     4561 cctcgccggc tttgttcgtc tctgccagga cacggccgta agggtcgagg atcatcgagt
     4621 tccccgtgcg gatctcatcg tcgtccacgc cgacgccgtt gctgaagatg agaaacatgc
     4681 cgttgtcgtg cgcgcggctc ggcagccagc gcatcagcca gccgcggccc ttttcgccgc
     4741 gcaactcccg ttcaacggcc gcaggattcg tgtcgcgctc gtcccagacc cggc
//
LOCUS       DATIFQ010000540         3738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_381233, whole genome shotgun sequence.
ACCESSION   DATIFQ010000540 DATIFQ010000000
VERSION     DATIFQ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381233"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VLE22_27170"
     CDS             complement(<1..799)
                     /locus_tag="VLE22_27170"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HSB18164.1"
                     /translation="MKHTRIIVTHYGGPDALRVVEEECPEPKNGEVRVRVLAAGVSLP
                     DLLMREGVHPETPRLPFTPGWDLIGVVDRLGAGVSGIEPGQIVAALPISGAYAEFVCL
                     PHRELVPVPSGLDPAEAVSLILNYVTAYQMLHRSVKVRPGHRLLIHGAAGGVGTALLQ
                     LGRLAGLEMYGTCSSQGASAVSDLGGIPIDYRQLDFVKEIHRLTGEGVDVFFDGIGGT
                     HIWRSRKALRPGGRVVAYGLTASLSGGRLASGRSGRRHRFRGIAIFGV"
     gene            complement(922..1134)
                     /locus_tag="VLE22_27175"
     CDS             complement(922..1134)
                     /locus_tag="VLE22_27175"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSB18165.1"
                     /translation="MRYAVVIERAENNDSAYVPDLPGCVATGATVEETEAQIREAIEF
                     HIAGLREDGVSIPQPSSRVDYVDVPV"
     gene            complement(1486..1683)
                     /locus_tag="VLE22_27180"
     CDS             complement(1486..1683)
                     /locus_tag="VLE22_27180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18166.1"
                     /translation="MIRNDQELEAMLARIRHFQVQLTHLRKVETNPANYRLSASGFLA
                     EIDRMQLEVREYLSLPAVEAA"
     gene            complement(1680..2081)
                     /locus_tag="VLE22_27185"
     CDS             complement(1680..2081)
                     /locus_tag="VLE22_27185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18167.1"
                     /translation="MHRASLREVLDRFGRGSRQRMVVAERLERVYRLAQTTGHLARFV
                     VFGSFVTDKLEPRDVDVFLMMNDPFDADTLPSETALVFDHAAADAHFGASVFWVRRLA
                     AFGGEQAAVEYWQVKREGGQRGIVEITEERP"
     gene            complement(2250..2726)
                     /locus_tag="VLE22_27190"
     CDS             complement(2250..2726)
                     /locus_tag="VLE22_27190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18168.1"
                     /translation="MDLRPTADAQRSAYVSRALALIMAALAAGVAVAGGDYVKGRVSG
                     FSGADGVYTFHFEQSDARPALEDGCPRFTVLVQYGRVPWYSWVPLVKTSHPTRGETDA
                     AAAILAVAERERRLVSFGYTGNGLIPSGGQCTSATKGLRIIKDDSGDVLMAFYDPT"
     gene            complement(2766..3113)
                     /locus_tag="VLE22_27195"
     CDS             complement(2766..3113)
                     /locus_tag="VLE22_27195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18169.1"
                     /translation="MTTVVLDVRSLQDTLAEASRVMKTGRAKPEARISFATPELLWAV
                     LTAKRWELLKALCGAGPVSIREASRRVERDVKAVHGDVKALLEAGLLNRTPNGRIEFP
                     FDAVKVEFLLGAA"
     gene            complement(3094..3375)
                     /locus_tag="VLE22_27200"
     CDS             complement(3094..3375)
                     /locus_tag="VLE22_27200"
                     /inference="COORDINATES: protein motif:HMM:NF039803.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HSB18170.1"
                     /translation="MLRRRVIVAADAFVEVAVWKVPEPVSSSTHAFRYRLAYVVGGHC
                     VLRYDNERGKGDHRHTETTEAPCPFSTPDQLLADFAADVARWNHDHGRS"
BASE COUNT          722 a         1236 c         1119 g          661 t
ORIGIN      
        1 acaccccgaa gatagcgatt ccacgaaagc gatgacgacg acctgaacga cctgaagcca
       61 atcggccccc acttagcgag gcagtaagac cataggccac taccctcccg cccggacgga
      121 gagccttgcg ggaccgccag atatgggtgc caccgatgcc gtcaaagaag acgtccacgc
      181 cctcacccgt gaggcggtga atttctttca cgaagtcgag ctgtcggtaa tcaatgggga
      241 taccgcccag gtcggaaacg gccgacgccc cttgtgatga acaggtaccg tacatctcca
      301 gcccggcgag gcgcccaagc tgcaataatg ccgtgccgac cccacctgcc gcgccgtgaa
      361 tcaacaggcg atggcccggt ctgaccttga cggagcgatg cagcatctgg tacgccgtga
      421 cgtagttcag gatcaggctg acagcctctg cggggtccaa cccagatggc accggaacca
      481 gttcacggtg cggcaggcag acgaactccg cgtacgcacc gctgatcggc agcgcggcga
      541 ctatctggcc tggttcgatt ccggagacac cagcgccgag ccgatccacc acgccaatca
      601 gatcccagcc cggcgtgaag ggcagccggg gcgtttcggg atgaacgccc tcgcgcatca
      661 gtaggtcggg gagggagacg cctgcggcca gcactcgcac ccgcacttcg ccattcttcg
      721 gctcggggca ttcttcttca accacccgaa gtgcgtcggg cccgccgtag tgagtgacga
      781 tgatgcgcgt gtgtttcacg aaccgcttcc tcgcttgccg cgcgctcgac cttgtgcggc
      841 gtaacgttcc agggtaaccg gcgcccccca gctgacacct gcgagacacc gtgttcagcg
      901 ggcgcccggt tgaccctgtt gctataccgg aacgtctacg tagtctacgc ggctcgacgg
      961 ctgaggaatg gaaacgccat cttcgcgaag acctgcgatg tggaactcaa tggcttcacg
     1021 gatctgagcc tcggtctcct cgactgtcgc acccgtggca acgcagccgg ggaggtctgg
     1081 cacgtatgcg gagtcattgt tctccgccct ctcgatcaca accgcgtatc gcatgggaac
     1141 ctcacttcct gagcccggcc tgcttgggat gctgttcaac ctaccagggg ccagatcatc
     1201 attcggcttc ccggggacgg tcacccggcc aggcttcgac gggtgtttgt actgtcgatg
     1261 gctgcctcgt gtggcgacaa ggtaccagcc atcatcctgg agccttcgga gaacctcgct
     1321 gactttcatc caatcacact ctccagacaa ttcgaactcg gcaagagcaa cggcggcggt
     1381 caccgggccg caggcaagca atcatacttt ggcaagtgac cggaccgcgg ctccggtgaa
     1441 tcgcctggtt cgccgatcga gcgcgatccc ccggccctcg gaaggtcacg ccgcctcgac
     1501 ggcgggcagg ctgaggtatt cgcggacctc caactgcatc cgatcgattt ccgccaggaa
     1561 tcccgatgcc gacaatcggt aattcgccgg gttcgtttcg acctttcgaa gatgcgtcaa
     1621 ctggacctgg aagtgccgaa tgcgcgccag catcgcctct aactcttggt cgttcctgat
     1681 catggcctct cctcggtgat ctccacaatg ccgcgctggc ccccctcccg cttcacttgc
     1741 cagtattcta cagcggcttg ctcgccgccg aaagcggcca gacgacgcac ccagaacacg
     1801 ctggcaccga agtgtgcgtc ggcggcggca tggtcgaaca ccagcgccgt ttcgctgggt
     1861 agtgtgtcgg catcgaacgg gtcgttcatc atgaggaata cgtccacatc gcggggctcc
     1921 agtttgtccg tcacgaacga cccgaacacg acgaaccggg ccagatgccc ggtggtctgt
     1981 gccaagcgat acacgcgctc gagccgttcc gcgaccacca ttcgctgtcg agagcccctg
     2041 ccaaaacgat cgagcacctc gcgcaatgat gcccgatgca caccgggtgg caagtcccct
     2101 acacctgtga acggtggcaa cggcacccga gacccccatt cactgtcgcc gaacgtacgc
     2161 ctaatccgcg tgagagacct gcgcggcact tgcggcattg tgcaagggcc gtgacggacc
     2221 gtgcggcggg ttcaggcggc tgttcggcgt catgtcgggt cataaaaggc cataagcaca
     2281 tcgccggaat catccttgat aatgcgaagc cctttggtcg cggacgtgca ttgccctccg
     2341 ctgggaatga gaccattgcc cgtgtatccg aagcttacga gacggcgctc gcgttcggca
     2401 acggcgagaa tggctgccgc ggcgtcggtc tcaccgcggg ttggatgaga tgtcttcacg
     2461 agggggaccc aggagtacca tgggacgcgg ccgtactgga ccaacacggt aaaacgggga
     2521 cagccgtctt cgagcgcagg ccgcgcgtcg ctctgctcga aatggaacgt gtaaacgccg
     2581 tcggcccctg aaaagccgct tactcggccc ttcacatagt cacccccggc tacggccaca
     2641 cccgccgcga gcgctgccat gatcagggcg agagctctac tcacgtacgc cgaacgctgg
     2701 gcgtcagcgg tgggccgaag gtccatccgg ctgcacgcca gggtttggca ccggccccct
     2761 ccggctcacg cggcgcccag gaggaactca actttcacgg cgtcgaacgg aaactcaatg
     2821 cgcccattgg gcgtgcggtt cagtagcccc gcttccagga gcgccttgac gtcaccgtgg
     2881 acagctttca cgtcgcgctc cactcgacgc gaagcctcgc ggatcgatac ggggccggcc
     2941 ccgcacaggg ccttgagcag ctcccagcgc ttggcggtca ggactgccca gaggagctct
     3001 ggggtcgcaa agctgatccg cgcctcgggc ttggcacggc ccgtcttcat tacgcgcgag
     3061 gcctcggcca gcgtgtcctg gagcgaccgc acgtcaagaa cgaccgtggt catgattcca
     3121 cctcgcgacg tcggcggcga agtccgccag cagttgatca ggcgtagaga aggggcaggg
     3181 cgcttccgtt gtttcggtgt gccggtggtc gcccttgccc cgctcgttgt cataccgcag
     3241 cacgcagtgg ccccccacga cgtacgcaag ccggtacctg aatgcatgcg tcgatgacga
     3301 taccggctct ggcaccttcc acaccgcgac ctcgacgaag gcgtccgcag caacgatcac
     3361 tcggcggcgg agcacagcgg tagccttcat gttggagaga gtaccaacac cgaggcctgt
     3421 tgtcaattac tccagcactc gccgtccgac gacttggtgc ctaacgcctg aattaagccg
     3481 cgccgcgaag cggcgtcggc ttgaatgaat tgttaggcct gcttgagaca gacctttctg
     3541 ttgattgcac ggataactag accataagaa atgaattcag gcgcaatcac atctcggcga
     3601 gccaccagct gttgcgcggc acagccgttg acgatccgcc ggatgcgcca acgcatcacc
     3661 gcggcatcag cgcgaacaca ccccagccca ggtattcacg cgtgtaagcg gcgtagcgct
     3721 cgggttccga ggtcagtt
//
LOCUS       DATIFQ010000541        30383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_571573, whole genome shotgun sequence.
ACCESSION   DATIFQ010000541 DATIFQ010000000
VERSION     DATIFQ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 30383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30383
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571573"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VLE22_27205"
     CDS             <1..591
                     /locus_tag="VLE22_27205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB18171.1"
                     /translation="SQLPEQQVRRLTRNMYNASVRIQELLQDYVDLCRTRESQPHPSN
                     LRGLVANAVDRIAPVAESQSVVVVHDVPADLVVTLNRRRIGSVLANLLTNALEAMPTG
                     GSIHITAMVAASSVVIRVLDTGPGIAPEIRERLFQPFVTARKPNGWGLGLAHARQVVI
                     DHGGEMWLESPPGWGACFAFSLPASSRSCPLKVDHR"
     gene            623..2758
                     /locus_tag="VLE22_27210"
     CDS             623..2758
                     /locus_tag="VLE22_27210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HSB18172.1"
                     /translation="MRLLCGAGHRFPWPASFLPVFSALLLVTPLFAAAGGGATVIDSA
                     GNVWRTGQANLIETTATAFQKTAVSSVCATENPSPFQGPIPVYCQHAYLIKQDPSGNV
                     LYATYLGGSSQDGATAITTDALGNVYVTGYTYSADFPVTTGVVQSTNAGPLTPAVVMG
                     TQFPYGPTYVAAGGDVFVAKFASDGALLFSTLVGGSGSDVPSLIAADSSGSVYVSGAT
                     ASTDFPLTAGAITRQATTSFFLRLNANGTTLTYSTYSAPSILAFDVDNQGRAYLTGDF
                     APPSSSVTSGPYVTIVDTQRGTVLNSTYLPAIAPEIAGAGLAIAVNAEQNLFLAVSPA
                     PLPADPRSNLPPFRPLGAGYFLELSSDGRILAETDISQTRLDSLLLDASGNAYAFGRG
                     TGAIPATAPQMLALPCSPDGGAFVLQSNPAGSIVAATYFRQGDDTALSLTSPGHLLLY
                     RTRSSTTVPLDLTIQPPANFGCPKNLASNTAAQGLAPGEVFVLTGYDLGPAQGIAAVP
                     NAAGQYPTSLGGVQALFNSKPAPLLFVQANEIHAVAPFAFPDFPVIEVQYGAQSAPPL
                     DISSSTVDPGIFSANGQGAIINQDGTVNTPANPARLGSIVSIYATGLGTLVNDSRSTR
                     NPLADGQVTPIPPPYILTELAYPQVTFAGVAGVTLWSGAAPGLIAGVTQINVQLPAEL
                     PSGIELDAVPMILNAAATPSPPVLISVKQ"
     gene            complement(3055..5250)
                     /gene="katG"
                     /locus_tag="VLE22_27215"
     CDS             complement(3055..5250)
                     /gene="katG"
                     /locus_tag="VLE22_27215"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HSB18173.1"
                     /translation="MSTETKCPVTAASRHTAAGAPMNATWWPNQLNLKILHQNSPLSD
                     PMGKEFNYAEEFKSLDLHAVIKDLEVLMKTSQSWWPADYGHYGPLFIRMAWHSAGTYR
                     IGDGRGGAGSGTQRFAPLNSWPDNASLDKARRLLWPIKQKYGRKISWADLMILAGNVA
                     LESMGFKTFGFGGGREDVWEPEEDIFWGPEGKWLGDERYTGDRQLDNPLAAVQMGLIY
                     VNPEGPNRNPDPVAAARDIRETFARMAMNDEETVALIAGGHTFGKTHGAADPNKYVGP
                     EPEGALIEEQGLGWKNTFGSGCGADTITSGLEGAWTTTPTKWSNNFFWNLFGYEWELT
                     KSPAGAHQWRPKQGAGSGTVPDAHDPSKKHAPFMLTTDLSLRMDPAYEKISRRFLENP
                     DEFADAFARAWFKLTHRDMGPVSRYLGPLVPKEQLLWQDPVPAVDHPLIGEQDIAALK
                     AKILKSGLTISQLVTMAWASAATFRGSDKRGGSNGARIRLAPQKDWEVNQPAQLAKVL
                     EKLQAIQEEFNSAQSGGKKVSLADLIVLGGCAAVEAAAKKAGHDVNVPFTPGRTDASQ
                     DQTDARSFAPLEPAADGFRNYLRAGQTLSAEELLLDRAQLLTLTAPEMTVLVGGMRAL
                     NANCGQSKHGVFTNRTGTLTNDFFVNLLDMRTAWQASSTSEGVFEGRDRATGELKWTG
                     TRVDLIFGSNSQLRAIAEVYACEDATEKFVKDFVAAWNKVMNLDRYDLA"
     gene            complement(5322..5750)
                     /locus_tag="VLE22_27220"
     CDS             complement(5322..5750)
                     /locus_tag="VLE22_27220"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HSB18174.1"
                     /translation="MVDPEAVKRSLESCGLRCTPQRYALMAFLVEHNGHPTAAEIFEG
                     VNCVDPRSSRATTYNNLRDLVQAGLVREVAVEGRAARFDGKGTRHHHFICDHCGSVED
                     MEWYEVPKPASGSLGKRVLRECELILRGLCTKCSPRHASR"
     gene            complement(6070..6885)
                     /locus_tag="VLE22_27225"
     CDS             complement(6070..6885)
                     /locus_tag="VLE22_27225"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB18175.1"
                     /translation="MTLVWLPLILFVVLCLILGRNFIRFRGVLKAQLAAYRRGDYQGQ
                     LKIIEGFRTNRSEPEHYLFFRGTACYELGRLEEAEQLLRRSLSMANDPARRTVCRDQL
                     GLVLMEQARYDEAAACFRVCIEESPKRGGGHRGMAETLLRQGGENVPALDAARLAVEA
                     DRAEKVLGGKLGREAHVLNLSESLAVFAWALTKNSVDPAEVEGALTEAFALCGETTKP
                     IRARLHYCASQAFSALGNTAESTRHIQFAAEIDPLGNYGRLARAAAGTAIPPA"
     gene            complement(7045..7569)
                     /locus_tag="VLE22_27230"
     CDS             complement(7045..7569)
                     /locus_tag="VLE22_27230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18176.1"
                     /translation="MAISGATPLATGPQALSRHRLAVATAVVFVISSAFPVVAGLAKD
                     PASFPKWWGVADVAIAFVLAFLAIVISALVGHDVNRQAEEASYRAYRFLTHGILAMVV
                     AFLLFGDRITWSIWLVGLGWRTWLLFYSLPAWFTALRSYQPGCESHPDRRSRQHHGQG
                     SDVKSRELNANERG"
     gene            complement(7678..8475)
                     /locus_tag="VLE22_27235"
     CDS             complement(7678..8475)
                     /locus_tag="VLE22_27235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18177.1"
                     /translation="MRTFLLILISVSLLGEETKQERGKRIVDEALAALGGDKFLAMKD
                     RVEAGRAYSFYREELSGLSIAKIYTQYLTAANSAPTDFIGVRERQAFGKDEYSAVLFT
                     GDTGYQITFRGARPLPDSMLERYRQSAIHNIFNILRLRLGEKGLIFESRGTDVWLNQP
                     VEIVDITDSENRVVTVYFHLSEKVPLRQEFDRRDPKTRERIVEETEYGKYREVGDGVK
                     WPFTMLRKRDGEKVYEIFSETVTINQGLSDGLFVLPSNLKILKKPVE"
     gene            complement(8472..10598)
                     /locus_tag="VLE22_27240"
     CDS             complement(8472..10598)
                     /locus_tag="VLE22_27240"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSB18178.1"
                     /translation="MTRLIIATLAAAAVLGAQTAPAKAPAKQAPLPSYQTLKYPPLKE
                     VRIPEVATYTLANGMKLYLVENHELPLISGFALVRTGNLFDPPDKIGLADVTGTVMRT
                     GGTKRKTGDELDEQLENIAASVESDIGETSGRVSFSALKENTDEVLAVFKDVLTEPEF
                     RQDKLDLVKTQLRSAISRRNDSPGGIAAREFSEAVYGRDNSYGWRMEYEHVDRIQRDD
                     LIAFYRRYFFPANIMLSVYGDFSAPEMKARIEKLFADWNYTQPAVPPFPPVQQKAVPG
                     VFLATKEDVNQTFFYAGHLGGLLNDKNYPALEVMADILGGGFKSRLFKRIRTELGYAY
                     SIGADWGANYNHPGVFTISGSTKSASTAETLKVVREEIEKIRNSEVTDAELASAKDTV
                     LNSFVFNFDTPGKTLTRLVTYEYFGYPKDFIFQYQKGVAAVTRADVLRVAREYLKPEN
                     VTIVAVGKPAEFGEPLTALGMPVKAIDLTIPEPKQAAAKVDAASMERGRQLLQRLQAA
                     AGGAKKIAEVKDFTAVSTAEIQAGPMPMKASRTLRWLSPGHLRQEQELPFGKITAYFD
                     GTAGWLATPQGTMDLQGPFLKQTREEVFRFPMGLWLSDRVPGRTVNATAEDVIEVSDQ
                     QGNSVRVQLEPKTGLPLKQFYRTVTMQGPPSEVEESYSDWREVDGLRLPFAITLQQSG
                     RKIMDVSITDYKLNSGLKAEELSQKP"
     gene            complement(10595..12103)
                     /locus_tag="VLE22_27245"
     CDS             complement(10595..12103)
                     /locus_tag="VLE22_27245"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSB18179.1"
                     /translation="MMRLTRGAALVLVVCPLLVSAQNLQDLEKRVTEFTLPNGLHFIV
                     LERHEAPVVSFHTYVNAGSVNDPGGRTGLAHMFEHMAFKGTDTIGTKNYAGERKALDA
                     VEQVYDRLEAERNKGFRSDPEAVKKLEAELKAAIEKANSFVEPNVYPQIIEENGGVGL
                     NAGTGMDSTNYFYNLPANRIELWFLLESERFLKPVFREFYKERDVVREERRMRIESSP
                     QGRLMETMLATAFAAHPYRNTPGGWASDIESLRVGDARRFYETYYVPVNLTIGIAGDV
                     DPAQARRLADKYFSRLPVAPPPPRVNTEEPAQSGRKRAAVESPAQPFLMIGYKRPDQL
                     NADDVVFDVISSILSSGRTGMLYKELVRDKKLALGAMAIATFPGGKYPGMFLVYSVPN
                     AGRTVEENEEAIYGILEKLKKEKVDEATLARVKTKIRASLIRQLDSNSGMASQLTHYY
                     VNYGNWRKLFTGIEEINKVTADDVQRVARKYFFEDNRTVAFTVKPKAGEAGK"
     gene            complement(12653..13249)
                     /locus_tag="VLE22_27250"
     CDS             complement(12653..13249)
                     /locus_tag="VLE22_27250"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HSB18180.1"
                     /translation="MGTPEFPQRKPLNGVVVPRWLSFLLSLVIFLVLIPIAHGVVPWA
                     ISSLMPRNGWTDGHPGLWNLLGLTPVVFGGAMLIWVLVVAIAETPERVELALEVNHSI
                     PIATPSFLMMRGPYAFTRNPMYVAELGLWLGWAIFFGSVGVLFGFVLGFVLVNFLVLP
                     REERGLERRFGALYLQYKNEVPRWLGKRNSISQHPARS"
     gene            complement(13351..14772)
                     /locus_tag="VLE22_27255"
     CDS             complement(13351..14772)
                     /locus_tag="VLE22_27255"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HSB18181.1"
                     /translation="MVSDRPKLVILSAFLSLFCTLFAAFGQTDTVVVRPKEVDTVLVN
                     PGMGIQTFQRFRNQEIYPGMRWSEVGPTAAVPDASGKVDFPESSMAYVRWFWSQLEPE
                     QGKYRWDIIDNALEEARKHGQTLMFRLMPYDQRNPLPEWYRNSGARRANKPTDKDGEI
                     WSPDADDPLYAKHWNALVTEAGQRYDGHPYLDSVDVSTVGYWGEGWGPYLPSMAVQKA
                     LIDANFEAFKRTPLLMNFDEPTALAYGTGKGAGWRLDCWGDLGGRGRGMMHMLDMYPQ
                     QVVRTGIQDVWERSPVSLETCGTAMSWKQWGYSDKDIDYILAQALRWHASTINLKSVT
                     IPPEWRTKFTEFEKKIGYRFVLRKLQYPKRARIGHMMPVSMWWLNAGVSPIYHEHHLA
                     VQFHSASGAAAARVPVDIRKWMPGDAVYDGSLYVDHSLKPGTYRVRVAVLDPRTNEPA
                     VRLAIEGLQPDGWYDLGEITLGP"
     gene            complement(14778..16337)
                     /locus_tag="VLE22_27260"
     CDS             complement(14778..16337)
                     /locus_tag="VLE22_27260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18182.1"
                     /translation="MRRRDLFKTAGAVATLFARRASADIPTHRWDGYDFGPGPKVHDR
                     LNQGPFGIEQDEGWYTVLATTASDRPVKNFGLGLVGYTWEEGGPSLAARRGEEALERH
                     VEKLASLPFVDVLYIRCDWRHVQSRPGRLDLAPIWPLTLDAAKRRGLRVAFRVQLSSP
                     GIQPKQISMPDFLREKIPLANIGRVARRYEHDLLEPRYDHPEFRKAFRELNELLAAEF
                     DNNPLVEFVDLMQYGFWGEGHTSDLPSPFPDYLTAEKVFTGMTRLQLDAWKRTTLAVN
                     TQPDISKTGNREVIDMAVRAGCWLRSDSVIVDEPEQIEALSNRPPWLAVIMEDGGKRQ
                     YSLDPKALDSDAAGISRLENTMLHVLDLGGNYWSLWTEADNIRRFYERYPKALETMQR
                     RIGYRVRPSWVWQRKRYGTTELVVAFANDGVAGVPGVLCVSVETPDGKVKVGGGLDAG
                     HPHGGRLRQASFVLPKGLEGTRVVLKAELETKGGVRRPVRWACAQPLNPDGSLTVQLM
                     SATDPRWRKGV"
     gene            16560..16883
                     /locus_tag="VLE22_27265"
     CDS             16560..16883
                     /locus_tag="VLE22_27265"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSB18183.1"
                     /translation="MDENTFHDRRTHWAVTPVCPPVARAKRLSGTVHVRVLVDMSGRV
                     VKTCPIYKPTEPRADESLVDAAISYVEKGRFRPNLGSPKGSEAGFRYAATTVDIHFIL
                     ADQEK"
     gene            16964..17206
                     /locus_tag="VLE22_27270"
     CDS             16964..17206
                     /locus_tag="VLE22_27270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18184.1"
                     /translation="MRTTLTLDDDVANLLNKEIQRSGASFKKAVNHFLRLGLMAPKQQ
                     NRKRFVVTPRSLGLPPGMSYDNVEELIEAVEGAAHK"
     gene            17203..17631
                     /locus_tag="VLE22_27275"
     CDS             17203..17631
                     /locus_tag="VLE22_27275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSB18185.1"
                     /translation="MIVIDANILLYAYDSTSTHHTRARRWIERVLSGGTPVGVPWQTA
                     TAFLRIVTNPRLPGDRFTLDRAARIVDQWYEQPNVRLLAPGEGHWAILRRVMIDGQAR
                     GPLVTDAQLAALTIEYGGVLHTTDRDFARFPGLRWTNPLA"
     gene            17725..19944
                     /locus_tag="VLE22_27280"
     CDS             17725..19944
                     /locus_tag="VLE22_27280"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HSB18186.1"
                     /translation="MTNLNRRAFLMTSGAALGRAVLPAQQAVTAASYAEQYPDMLLRY
                     LSARLNALSERWDRERAAIRTAEAVAERNRFVRGKFVEMIHGYPERNPLRPVVVKVLE
                     RDGYRVENIMFESRPNFFVTGNLYAPTSGPGPFPAILSPCGHYPLARMQPDYQFVYLN
                     LVKAGFVVLGYDPIGQGERRYYWDPRTDETESALGPVYEHSMPGQLLLLLGENLSAYR
                     IWDGMRGIDYLLTRPEVDKEKIGCAGHSGGGTLTLFISALDERVRCAVVNEGGTGHRW
                     PVHIAPGGPFGPSDVEQNLFPSALYGADLCDLHVAIAPRPLLTLTEEYSPWFNLAAGH
                     IRDRYRQFGVAEKFATEEATDPHAYTVRLRQATTDWFSHWFYGRKGPEAEPDFDAEPR
                     RNLYCTPNGSVRYSQQGDTIFSLILKRQASLPEESKPPSSRREWEEWRGRFLPTLKEL
                     LRYKPVTGPLGVRPLVTTARKGYRVEKLEFLSEPDIYIPTWVFLPQTRNAETRAILYL
                     NDAGIAGEGLEGMEFGVLEELARKGHLVVAVEPRGTGLTAPEHSEGVSEAPEFRQLFD
                     TETAAAYMAWFMDESLLGMRVFDVIRSVDYALTRNDVDKRGLRAIGTGRGATWLLFAA
                     ALDPRIQATVCDRGLLSYKSLAQSDRYLYGADVFIPGVLKRLDLPQVAAAVADRSLTL
                     LSPLDPMKKLVDVSEATVAYDRTRRTYTGAQVGGQFRILRREPELSLSDQYLHAFET"
     gene            complement(19972..29130)
                     /locus_tag="VLE22_27285"
     CDS             complement(19972..29130)
                     /locus_tag="VLE22_27285"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HSB18187.1"
                     /translation="MSSSAVEERQRNGDNEYVLFSACARKSRVSRFVLGFYRSEEHGE
                     EALREVRTNGFRRSAVIHRASDGGLKHFHDGLGPRNRAAFGVGLALAVAMLATIAGIP
                     PLMAVLAALCGFLITWFGTLWLGLGMSDRVLRGYRGFVLPGESLVVVEATEDEAADVV
                     AVLRRIGHPSVLVIRPGPGFTASTETAGQRREPATMAELPDCAAELAASHELEASTRS
                     RPLLPILRECEVVIERARADLAEAARLEYGITHAAEWLLDNAYLIRSNIADIRHNLPD
                     NHNKILPVLTDRSCPVRLRVYHLAAELIDRTGHRLATETIISFLDAYQHQSPLTVAEL
                     WVFPLMLRLVLLERLRRLAELAILRQHMKELADFWADRLLNAAHRTPEQFEEMVGELD
                     RHGNELTPHFIARLGEQLHRDESALAPIQKWIQEKTEHRLADIIRGEHAEEANDLMLI
                     SSAIGSLRQLSELQYPKIVEAVSRMEEILRQDPAGVHARSDFATRDRCRRVVEAAARQ
                     SKFSECDVARLAVDLAQPAPPGSRERCVAYYLLDEGLPELEKRLRRRIRWSEAWRRFL
                     HRYPAFFYLSSLAALTAGLSGAFRFAAYSAGVTSQGILLFLGILALFPASELVTYLLQ
                     MFLTSILPPRVLPKMSFEEGIPEDCRTLVVVPMMLLTPESVRGEIEKLEVRYLANPDS
                     NLWFSLLSDFTDAEEREMPEDDGLLGLAMKSIEELNSRHGHGRFLLFQRPRMWCETEG
                     RWIGWERKRGKLEELNSFLNGGKNTELLCVGSLPEGIRYVITLDADTELPHGSAQKLV
                     ETIAHPLNRVELTADERNHARGYTIIQPRVSITLPSATATRFTRLFTDARGTDPYCQA
                     VSDVYQDLFGEAIYHGKAVYDVRAFHKVLNGRFPEQRLLSHDLIEGAYVGVGLATDVE
                     LFEQFPYDYTSYSKRQHRWIRGDWQIASWILPRVPAATRPEDARNPLTLINRWKILDN
                     LRRSLVPVASLLLLVFSWTFHAAPAAASALVALTLVVPLLFQFLDRLVARWRGDAWAW
                     REANSDLNLAIVKAAFLPHQAYLSADAIVRVLYRLGISKRRLLEWQTAEMSYHAARLH
                     LDPFRIHFFLICAVTAGFMLSLALRGRLWEPAWAPFLVLWVAAPAVQHWIRSQRRALR
                     KAEQIELNDQRFLRRIARETWRYFDDLVGPEHNWLPPDNSQEALRVEVADRTSPTNIG
                     MWLVSAVSAHDLGFLTPEPMIDRCTATIETLAKLESCEGHLLNWYNTRTLEPLPPKYV
                     STADSGNLIASLWVLQQASQELDAESQLDGRALRGLADTLAVITERFQPDHSNRVSLA
                     ALRGLFEEESSGIEILERIRLAAEPARKLTDSLRWSVSTTGEPAYWFTRLERQIESWI
                     QHADLYLRWLDVLAAPPDEFLQPLGDSAIAARRRLLRELPSWGAVARGETGLLSEILK
                     SAGNKDGLSANLTAWIADVGAEYEKARKASADLLDRARHLGETSGALADGMDMQFLYD
                     AHRRLFAIGYQVGGPLNLSAHYDLLASEARLASLVAIAKGDVPVEHWLALGRPYTSAD
                     GQVLLSWSGTMFEFLMPLLFTRSFRNSLLENACAVAVKRQIEYTKDRGVPWGMSESAY
                     SALDSHKIYQYRAFGVPSLGLKRGLEEDLVIAPYATALALLVNPAESIKNLKRLVKAG
                     MYGRMGFYESLDYTRQEERRGGKGVIVYAYMAHHQGMSLVALNNVLNRGIMRQRFHAD
                     RRIKAVEPLLFERIPPVPSMLVHRPSDQVAMRLISGPSAPEYRVFDEDTPIPQVQLLG
                     NGRYALMITNTGAGYSRWGEFDITRWRSDTTRDNWGTFFYLREEESNTLWSVTHQPLN
                     VKDPRYTATFSADRAEFRRRRLGIESHLEVTVSPEDDAEIRRITLVNHGSRARTLELT
                     SVAELSLAPHGADRAHPAFNKLFIQTEARPDLHALLAWRRLRSPDESPIWVAQLMMES
                     PKDQSSFQYETDRARFLGRGRTWSDPAMSLKQTDGYVLDPVFVMRQRVSLEPRQQAQI
                     TLVTVAAGSREDLMRLVVKYRDSDVCSRAFELAWSHAQLEYRYLGIQSDAAMRFAELA
                     GHLLYPNVRLRAPAERLRRNVQTQSKLWSHGISGDLPLAAVWVGDSQGLTLVRELLVA
                     HTYWRLRGLKADLLVLNREPASYEQPLHQQLLRLVEAHSLHTGVQQPGGVFLRKADQI
                     SEEDLNLILTVAQATVGTVRGTLSKQLSSPPEGISLPPPLRIRRREEQPSAPLPFLEL
                     AYFNGLGGFTADGREYAIYLGPNAFTPLPWINVMANSVFGALVSEAGSGCCWYGNSQS
                     NRLTPWSNDPVSDPSAEAIYIRDEDSGVFWTPTPLPVRELDAYRARHGQGYTEHEHTS
                     HALEQTLVTFVPVHAERADPVRIQRLRVRNNSSQRRRLSVTSYSELVLGTDREATQMH
                     VASSWDESAKALFSRNPYHLDYGGRVAFAAMTPEASSYTGDRTAFLGRNGSVEAPAAL
                     RLESLSKRTGPGLDPCGALQTKFDLGPGEEKTVTYVLGQADDAEHAGRLIARYRDAEN
                     VERSLNETREWWDDLLTTIQVKTPILSVDFMLNRWLLYQSLSCRIWGRSALYQSSGAY
                     GFRDQLQDALALVYSAAPIARELILRAASRQFTEGDVQHWWHLPSGAGVRTRCSDDLL
                     WLPFAVCQYMDVTGDAGILNAATTFLEGPLLKEEEREVYFQPSVSIDQVTIFEHCRRA
                     IEKGVTSGPHGLPLIGMCDWNDGLSSVGDEGKGESVWLAWFLVDVLKKFAEVCAGRGE
                     QELASRYRGRADRLSATVERTSWDGEWYLRAFFDDGTPLGSRRNEEARIDSLSQSWSV
                     ISGAGRPDRAAEAMRSVEEHLIREKEKLVLLCTPPFDKSSPHPGYIMGYPPGVRENGG
                     QYTHAALWVAMAFARMRDGDRAVKVLQMLNPIEHSRTPEDYAAYRTEPYVVAADVYSL
                     QSQTGRGGWTWYTGSAGWMYRVWLEEVLGFKLRAGRLSIDPVIPEDWPGYVLTFRYGR
                     TEYRIEVENGGERSEQEIQLVDDGESHAIHICVGAPVSRTLVEAADPPTA"
     gene            complement(29279..30265)
                     /locus_tag="VLE22_27290"
     CDS             complement(29279..30265)
                     /locus_tag="VLE22_27290"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HSB18188.1"
                     /translation="MYDQAMDGDLSNGLPFLTTEGNRIVRAGTRETVLLRGLNRSGLE
                     YSEPDEQGFLSAAEISRNEIRTIVQEWGANVIRLPFNQDWVLRGRGGWTADAYCQALD
                     QMIRWASGFGAYTLLDLQWLDADRAYGGDRNFVAPLPNMQSIELWDLLATRYRGEPAV
                     LYDIFNEPHDRLPDDPYPLNREDGTTYPPGLYRVTMAEWQPWARKLIAAIRDVNPDSL
                     IFVGGTNWAYDLRGMPMDIPRLVYSTHVYPIKGRNWHEAFGHLAATVPVFAGEWGGAE
                     SDLKWGRKLLDYFDSLQMGWAAWSWHNAPLIVERYTATSFGRIVRARLSAET"
BASE COUNT         5788 a         9633 c         9052 g         5910 t
ORIGIN      
        1 tcgcaactcc ccgagcagca ggtccggcgc ctcacgcgca acatgtacaa cgcctcggtt
       61 cgcatccagg aactgctcca ggattatgtc gatctatgcc ggacgaggga gagccaacct
      121 catccatcca atcttcgcgg cctggttgcc aacgccgtgg acaggatcgc gcccgtggcc
      181 gaatcgcagt cggtggtagt ggtacacgac gtacccgcgg atctggtcgt caccctcaac
      241 cgccgtcgaa tcggctctgt tctggccaat cttctgacga acgcccttga ggccatgcca
      301 acgggcggat cgatccatat caccgcgatg gttgcggcga gttcggtggt gatcagggtt
      361 ctcgacaccg gacctggaat tgccccggag attcgcgagc gcttgttcca acctttcgtc
      421 accgcccgca aaccgaatgg ttggggcctg gggcttgcgc acgcacgcca ggtcgtcatt
      481 gaccatggcg gcgagatgtg gctggaatct ccacccggtt ggggagcctg ttttgcgttc
      541 agcctgccgg cctcgtcgcg gagttgtcca ttgaaagtgg accaccgcta gtgcaatgcg
      601 tcacaagaaa tgcgacttat ctgtgaggtt gctttgtggc gcaggccatc gctttccgtg
      661 gcctgcgagt ttcctccccg ttttctccgc tctgctcctc gtcaccccct tgttcgcggc
      721 tgcaggtggt ggcgcgaccg tcatcgacag cgccggcaat gtctggcgaa ccggccaggc
      781 gaacctcatc gagaccaccg caaccgcgtt tcagaagacc gccgtctcct cagtctgcgc
      841 cacggagaat ccttcgccct ttcagggacc catccccgtc tactgccagc acgcttactt
      901 gatcaagcag gacccttccg gcaatgtcct ttatgccact tacctgggag gcagcagcca
      961 ggatggcgcc accgccatca ccaccgatgc gctgggtaat gtctacgtca ccggctacac
     1021 ctactccgcc gacttccccg tcaccaccgg cgtcgtgcag tccacaaacg ccggcccgct
     1081 cactcccgcg gtggttatgg ggacccaatt cccgtacggc cctacatacg tcgctgccgg
     1141 cggggacgtc ttcgtggcta agttcgcctc cgacggagcc ctgctgtttt ccacgctagt
     1201 cggcggctcc ggttccgacg tcccctctct gatcgccgcg gactcctctg gctccgtgta
     1261 cgtctccgga gccaccgctt ccaccgattt ccctctcacc gccggggcga taacccgtca
     1321 ggccaccacc tccttcttcc tacgcctgaa cgcaaacggt acgacactta cttactccac
     1381 ttactccgct ccctccatcc tggcattcga tgtcgataac cagggccgag cctatctcac
     1441 gggagacttc gcgccgccgt cttcttccgt caccagcgga ccctacgtca ccatcgtcga
     1501 tacgcagcga ggcaccgtac tgaactctac gtaccttccg gcgatcgccc cggaaattgc
     1561 cggcgccggc ctggccatcg ccgtcaatgc cgaacaaaac ctcttcctgg ctgtgagccc
     1621 cgctcccctg cccgccgacc cgcgctcgaa cctgccgcct ttccgcccgc tgggcgccgg
     1681 ttacttcctc gagttatcct cggatggccg catcctggct gagaccgata tcagccagac
     1741 ccggctcgat tcccttctgc tcgatgcttc cggcaatgcc tacgccttcg gccgcggaac
     1801 cggcgcgatt cccgccactg cccctcagat gctggctttg ccctgctccc cggacggcgg
     1861 cgcctttgtg ctccaatcca atcccgccgg gagcatcgtc gccgccacgt acttccggca
     1921 gggggatgac actgcgctct ccctcacgtc tcccggccac ctcctgctct accgcaccag
     1981 gtcctctact accgtgccgc tggatctgac catccagccc ccagccaact tcgggtgtcc
     2041 gaaaaacttg gcgtccaata ccgccgccca gggactcgcc ccgggcgagg tcttcgtcct
     2101 caccggttac gacctcggtc ccgcgcaagg tatcgcggca gtacccaatg ccgccggcca
     2161 ataccccaca tctctcggtg gcgtccaggc actgttcaac tccaagcccg cgccccttct
     2221 gttcgtacag gccaatgaga ttcatgccgt ggccccgttt gctttcccgg acttcccggt
     2281 catcgaggtg cagtatggcg cccaaagcgc gccgccgctc gatatttcct cctccaccgt
     2341 cgacccgggc attttcagtg ccaatggaca aggcgccatc atcaaccagg acggcaccgt
     2401 caacactccc gccaaccccg cccgccttgg atccatcgtt tcgatctacg ccaccggcct
     2461 gggcacactg gtgaatgatt cgcggtctac ccggaatccc ctcgccgatg gacaggtgac
     2521 ccccatcccg cctccgtaca ttctgaccga gctcgcatac cctcaggtga cctttgccgg
     2581 cgtggccggc gtcactctgt ggagcggtgc ggcgccgggg ttgatcgccg gcgtgaccca
     2641 gatcaacgtg caactgcccg ccgagctacc atccggaatt gaactcgacg ccgtgcccat
     2701 gatactcaat gcggcggcca ccccgtcccc gcccgtgctc atcagcgtga agcagtaagc
     2761 aatcacggcc atctgtctgg cttactccct cactccagcg gcctcttcag gatcttcagg
     2821 ttcaagggcg atgggatcgg cgttacgcct caaatagcgg cagacctgac tcccggatgg
     2881 cgcgttgccg cttccacgcc gctccatgac ttcagcgatt caggctctgg cggaatgagc
     2941 gggcgcgcgg gccggatcgc gcgcccagga agaagcgacg aacttcagtt ttgccgatcc
     3001 aagcagcagg aacggaaatt cgtaccaagt cggcggctgt ccttctcgcc gcgcttaggc
     3061 aagatcgtag cgatccaggt tcatcacctt gttccaagct gccacgaagt ccttcacaaa
     3121 cttctccgtc gcgtcctcgc aggcatagac ttccgcgatg gctcggagct gggagttcga
     3181 accgaagata aggtcgacac gggtgccggt ccacttgagt tcgccggtcg cgcgatcgcg
     3241 cccttcaaac acgccttccg atgttgagga ggcctgccac gccgtcctca tgtcgagcag
     3301 gttgacgaag aaatcgttcg tcagcgtccc ggtccggttg gtgaagacgc cgtgcttgga
     3361 ctgcccgcag ttggcattca gggcgcgcat gccgccgacg agaaccgtca tctcgggggc
     3421 agtcagcgtc agcaactgcg cccgatccag cagcagctct tcggcggata gggtctgtcc
     3481 tgcccggaga tagttccgga acccgtcagc ggccggttcg agcggggcaa aggagcgtgc
     3541 atcggtctgg tcttgcgaag cgtccgtgcg gcccggcgtg aaaggtacgt tcacgtcgtg
     3601 tccggccttc ttcgccgccg cctcgacagc cgcgcagccg cccaggacga tcaggtcggc
     3661 gagcgagacc ttcttgccgc cggactgagc agagttgaac tcctcctgga tcgcctggag
     3721 cttctcgagc accttcgcca gttgcgccgg ctggttgact tcccaatcct tttgcggtgc
     3781 caggcgaatg cgggccccgt tcgacccacc gcgtttatcg gagccgcgga acgtcgccgc
     3841 cgacgcccag gccatcgtga ccagttggga gatcgtcagt ccggatttga gaatcttcgc
     3901 cttcagagcg gcaatgtcct gttccccgat cagtggatga tcgaccgccg gcacagggtc
     3961 ctgccacagc aactgctctt tggggaccag tgggcccaag tatcgcgaga ccggccccat
     4021 gtcgcggtgc gtgagcttga accaggccct ggcgaacgcg tctgcaaact catcgggatt
     4081 ctcgaggaag cgccgtgaga tcttctcgta agccggatcc attctcaggg agaggtcggt
     4141 ggtgagcatg aacggggcgt gcttcttgga gggatcgtgg gcatcgggaa cggtgccgga
     4201 tccagcgcct tgcttgggcc gccactggtg cgcaccggcc gggcttttgg tgagttccca
     4261 ctcgtagccg aacaggttcc agaagaagtt gttgctccac ttggtcgggg tggtggtcca
     4321 tgcgccctcc agcccgctgg tgatcgtgtc ggcgccacaa ccgctgccga aggtgttctt
     4381 ccagcccagg ccttgctcct cgatcagtgc gccctcgggt tcgggaccga catacttgtt
     4441 cggatcggcg gcgccgtggg tctttccgaa cgtgtgtccg ccggcgatca gcgctacggt
     4501 ctcctcgtcg ttcatcgcca tgcgggcgaa cgtctcccgg atatcccgtg ccgcggcgac
     4561 cgggtccgga tttcgattgg gcccttctgg attcacgtaa atgaggccca tctgcaccgc
     4621 agcgagagga ttgtcgagct gacgatcgcc ggtgtagcgc tcgtccccca gccacttgcc
     4681 ctctggcccc cagaagatgt cctcttcggg ctcccagacg tcctcgcgcc cgccgccaaa
     4741 accgaaggtc ttgaatccca tcgactccaa ggcgacgttg ccggccagaa tcatgaggtc
     4801 ggcccaggag atcttccggc catacttctg cttgatcggc cagagcaacc ggcgcgcctt
     4861 gtcgaggcta gcattgtccg gccagctatt gaggggcgca aaacgctgtg tgccggagcc
     4921 tgccccgccg cggccgtcgc cgatgcggta cgtgcctgcg ctgtgccacg ccatgcgaat
     4981 gaaaaggggc ccatagtggc catagtcggc cggccaccag gactgcgacg tcttcatcaa
     5041 aacctcgagg tccttgatca ccgcatgcag gtcgaggctc ttgaattctt cggcatagtt
     5101 gaattccttg cccatcggat cggacagagg agagttctgg tgcaggatct tcaggttcag
     5161 ttgattcggc caccaggtcg cattcatcgg ggccccagcc gccgtgtgtc tgctagccgc
     5221 tgttaccggg cacttggttt cggttgacat gttctactcc ttcctggaat gaatctaaaa
     5281 gaccgcgatg aactacaaac ccatctccac gcctctcacc gttaacggga agcgtgccgc
     5341 ggagagcact tcgtgcagag cccccggaga atgagttcgc actctcgaag gacccgtttg
     5401 ccgagggagc ccgaggcagg cttgggcacc tcgtaccact ccatgtcctc aacactgccg
     5461 cagtggtcgc agatgaagtg gtgatgccgc gtgcctttcc catcgaatct cgcggcgcgg
     5521 ccctcgacgg ccacctcacg caccagaccc gcctgtacca ggtcccgcag attgttataa
     5581 gtcgttgccc tggaagagcg cggatccacg caattcacgc cctcgaagat ctccgcggcc
     5641 gtaggatgcc cgttgtgttc caccaggaat gccattaagg cgtagcgctg cggagtacac
     5701 cgcaacccgc aggattccaa ggaccgttta accgcctccg ggtctaccaa atagttagat
     5761 tatgtttaat ttgagaagtt gtcaagatta cgctttggct tgccgttggt ggctttacct
     5821 caccaccacc ccaggcacat gctttgcagc ggattctttg cgtgcttcac ccgcttggcg
     5881 ggaaacgttt ttcacccgcg tccatccgcg ttcattcgcg gcttactacc tcactccacc
     5941 ggcttcttca ggatcttcag gttcgacggc agtaagaagt ccgagtccgg cacgcggcag
     6001 gatggccaag tttcgcgccc gtgacctacc agacgcggtc ggcacgtgcg gcaagcggaa
     6061 ggttagaggt tacgcagggg gaatagcggt tcctgccgca gcccgagcga ggcggccgta
     6121 gttgccgagg ggatcaatct cggctgcgaa ttggatatgg cgtgtacttt cggcggtatt
     6181 tccgagggcg gagaacgcct ggctcgcaca gtagtggagt ctggcgcgta tgggcttcgt
     6241 ggtctcgccg cacagcgcga acgcctcggt aagtgcgcct tcgacttcgg cgggatcaac
     6301 cgagttcttg gtaagggccc aagcgaaaac cgcgagcgac tcgctcaggt tgaggacatg
     6361 ggcctcgcgg cccagttttc cgccaagcac cttttcagca cgatcggcct cgacggccag
     6421 tcgcgcagcg tccagggccg gcacattctc tccaccttgc cgcaacaggg tttcggccat
     6481 cccgcgatgg cctcccccgc gctttggaga ttcctcgatg cagacccgaa agcaggcagc
     6541 cgcctcgtcg tagcgcgcct gctccatgag taccagcccg agttggtccc ggcagacggt
     6601 ccgtcgcgca gggtcgtttg ccatggacaa gcttcggcgt agcaactgtt cggcttcttc
     6661 caggcgcccc agttcgtagc acgctgtgcc gcggaagaac aagtagtgtt caggttcgga
     6721 tcggttggtt ctgaagccct caatgatctt tagctggccc tggtagtcgc cacgtctgta
     6781 ggccgcaagc tgagccttca ggacaccgcg aaaacgaatg aaattcctgc ccaaaatcag
     6841 gcaaaggaca acaaacaaga tcagcggcag ccagacgagc gtcatcgggg gaccagtata
     6901 tagcagatgc tggcgaaaac accggcctgg atgcagatcg caacctggtt agtgagatcg
     6961 taacgcggtg aagcacgcag gcacctcaga cacacgcttt gcagtggact ttttgcgtgc
     7021 ttcgcgcatt gcgggaaact tctttcatcc gcgttcattc gcgttcaatt cgcggctttt
     7081 gacatcactc ccttgcccat gatgctggcg ggaacgccgg tctggatgcg attcgcaacc
     7141 gggttggtaa gatcgtaacg cggtgaacca tgcaggcagg ctgtaaaaga gcagccaggt
     7201 gcgccagcca agtccaacta gccaaatgct ccaagtgatc cggtccccaa agaggaggaa
     7261 tgccaccacc atcgccagta tcccgtgcgt caggaagcgg taggcgcggt aactggcttc
     7321 ctcagcctgc ctgtttacgt catggccaac gagcgcactg atcacgatcg ccagaaacgc
     7381 aaggacaaac gcgatggcga catcggccac gccccaccat tttgggaagg acgccgggtc
     7441 ttttgcgagg cccgcgacaa cggggaaggc cgaggatatg acgaagacaa ccgctgtggc
     7501 gaccgcaagc cgatgacgcg aaagcgcctg gggcccagtc gcgagaggag ttgcacctga
     7561 aattgccatt gcggctcccc gtttgattca gcctacaccg agccgtccgt gtacagaacg
     7621 ccccaggggt tcacttcact attcgcgtgt atccgcgttc attcgcggct tactacctca
     7681 ctccaccggc ttcttcagga tcttcaggtt cgagggcagc acaaacaacc cgtcggagag
     7741 tccctggttg atcgtcaccg tctcggagaa gatctcgtac accttctcgc cgtcccgctt
     7801 gcgcagcatc gtgaacggcc acttcacgcc gtcgcctacc tcgcggtact ttccgtactc
     7861 ggtctcctcc acgatcctct ctcgcgtctt cggatcgcgc cggtcgaatt cctgccgcag
     7921 cggcaccttc tcggaaaggt ggaaatacac cgtcaccacg cggttctccg aatccgtaat
     7981 gtccacaatc tccaccggct ggttcagcca cacgtccgta ccccgcgact cgaagatcaa
     8041 ccccttctcg cccagccgca gccgcagaat attgaagatg ttgtggatcg cgctctggcg
     8101 gtaccgttcg agcattgagt ccggcaacgg ccgcgccccc cgaaaggtga tctgatatcc
     8161 ggtgtccccc gtgaacagga ctgcactgta ctcatccttg ccaaatgcct gccgctcgcg
     8221 cacgccgatg aaatccgtgg gcgcggagtt cgccgccgtc aagtactgcg tgtaaatctt
     8281 cgcgatcgaa aggccggaaa gctcctcgcg gtagaacgag tacgcgcggc cggcctcgac
     8341 gcggtccttc atcgcgagaa acttgtcccc gccgagcgcg gcgagcgcct cgtccacgat
     8401 gcgcttgccc cgctcctgct tcgtttcctc gccgagcaga gaaacgctga tgaggatcaa
     8461 caggaaggtt ctcatggctt ctggctcagc tcctccgctt tcagaccgct gttcagcttg
     8521 tagtccgtga tcgaaacgtc catgatcttg cgccccgatt gctgcagcgt gatcgcgaac
     8581 ggcagtcgca aaccgtccac ctcgcgccag tccgagtaac tctcctccac ctccgacggc
     8641 ggcccctgca tcgtcaccgt ccggtagaac tgcttgagcg gcagtccggt tttcggctcc
     8701 agttgcactc gaaccgagtt gccctgctgg tctgagacct cgatgacatc ctcggcggtg
     8761 gcgttcaccg tccgcccggg aacccggtcg ctcagccaca gccccatcgg gaaacggaat
     8821 acctcttctc gcgtctgctt caggaacggc ccctggaggt ccattgtgcc ctgcggcgtc
     8881 gccagccagc ctgccgttcc atcgaagtac gcagtgattt tgccaaacgg aagctcctgt
     8941 tcctgcctga ggtgacccgg cgaaagccag cgcagcgtgc gcgacgcctt catcggcatc
     9001 ggcccggcct ggatttccgc cgtcgaaacg gccgtgaagt ccttgacctc ggcgattttc
     9061 ttcgcgccgc cggcggctgc ctgcaggcgc tggagaagct gccgcccgcg ctccatcgag
     9121 gccgcgtcca ccttcgcggc tgcctgcttc ggctccggaa tcgtcaggtc gatcgccttc
     9181 accggcatcc cgagcgccgt cagcggctcc ccgaattcgg ccggcttccc gaccgccaca
     9241 atcgttacgt tctccggttt caaatactcg cgagccacgc gaagaacgtc cgccctggta
     9301 acggccgcga cgcccttttg gtactggaag atgaagtctt tcggataccc gaagtactcg
     9361 taagtcacca gccgcgtgag cgtcttgcca ggcgtgtcga aattgaaaac gaagctgttg
     9421 agcacagtgt ccttcgcgct cgcgagctcg gcgtcggtca cttccgagtt gcggatcttc
     9481 tcgatctcct cgcggacgac cttcagcgtc tccgcggtag aggcggattt ggtgctgccg
     9541 gagatcgtga atacaccggg atgattgtaa ttggcgcccc aatccgcacc gatgctgtaa
     9601 gcgtagccga gctcggtacg gatgcgttta aacagacggc tcttgaaccc gccgccgaga
     9661 atatcggcca tcacctcgag cgcggggtag tttttgtcgt tcaataatcc gcccagatgt
     9721 ccggcgtaga aaaacgtctg attgacgtcc tccttcgtag ccaggaagac ccccggaact
     9781 gccttctgct gcaccggcgg gaacggcgga acagccggct gcgtgtagtt ccagtccgcg
     9841 aagagcttct cgatccgcgc cttcatctcc ggcgccgaaa agtcgccata gacggagagc
     9901 atgatgttcg cgggaaagaa ataacgtcgg taaaacgcga tcaggtcgtc ccgctggatg
     9961 cggtcgacgt gctcgtactc catccgccag ccgtaggaat tgtcccggcc gtagacggcc
    10021 tcggagaatt cgcgcgccgc gatgccgccg gggctgtcgt tgcggcggga gattgcgctg
    10081 cgtagttgtg tcttgacgag atcgagcttg tcctggcgga actccggctc ggtcagcacg
    10141 tccttgaaca cagccagcac ttcgtccgtg ttttccttca acgcggagaa tgaaacccgg
    10201 ccgctggtct cgccgatgtc gctttcgacc gacgcggcga tgttctcgag ctgctcgtcc
    10261 agttcatcgc cggtctttct cttcgtgccg cccgtccgca tcaccgtgcc ggtcacgtcg
    10321 gccagcccga tcttgtccgg cgggtcgaag aggttgcctg tccggacgag cgcgaaaccg
    10381 ctgatcagcg gcagctcgtg gttttctacg aggtacagct tcatcccgtt ggccagcgtg
    10441 tacgttgcca cctccgggat gcgaacctcc ttgagcggcg ggtacttcag ggtctgatac
    10501 gacggcaggg gcgcctgttt cgcgggagct ttggccggcg cggtctgcgc gccaagaact
    10561 gctgcggcgg cgagcgttgc gatgatcagt ctggtcattt ccctgcctcc ccggccttcg
    10621 gcttcacagt gaatgcaacc gtccggttgt cctcgaagaa gtacttgcgg gccacccgtt
    10681 gcacgtcgtc cgcggtgact ttgttgatct cctcgatgcc ggtaaacagc ttgcgccagt
    10741 taccgtaatt cacgtaatag tgcgtcaact gcgaagccat gccggagttg ctgtcgagct
    10801 gccggatgag cgacgcccgg atcttcgtct tcacgcgggc gagcgtggct tcgtcgacct
    10861 tctccttctt cagcttctcg agaatcccgt agattgcctc ctcgttctcc tcgacggtgc
    10921 ggccggcgtt cggcacgctg tagaccagga acattcccgg atacttcccg ccggggaatg
    10981 tggcgatcgc catcgctccg agcgcgagct tcttgtcccg caccagttct ttgtagagca
    11041 ttcccgtgcg gccgctcgag aggatggagg agatgacgtc aaagaccacg tcgtcggcgt
    11101 taagctggtc cggccgtttg tagccgatca tgaggaacgg ctgcgccggc gactcgacgg
    11161 ccgcgcgctt gcggccgctc tgggcaggct cctcggtgtt gacgcgcggc gggggtgggg
    11221 ccaccgggag tcgcgagaag tacttgtccg ccaggcggcg cgcctgagcc ggatcgacgt
    11281 cgcccgcgat gccgatcgtc aggttgaccg gaacgtaata tgtctcatag aagcggcggg
    11341 cgtcgcccac gcggaggctt tcgatgtcgc tcgcccagcc gccgggagtg ttccggtacg
    11401 gatgcgccgc gaacgcggtg gcgagcattg tctccatcag cctgccctgc gggctggatt
    11461 cgatgcgcat gcgccgctct tcgcgcacca cgtcgcgctc cttgtagaat tcgcggaaga
    11521 ccggcttgag gaaccgctcg gattcgagca ggaaccacag ttcgatgcgg ttggccggca
    11581 ggttatagaa gtagttcgtc gaatccatcc ccgtgccggc gttcagcccg acgccgccgt
    11641 tttcctcgat gatctgcgga tacacgttcg gctcgacgaa ggagttggct ttctcgatgg
    11701 cggccttcag ttcggcttca agcttcttga ccgcctccgg gtcggaacgg aagcccttgt
    11761 tgcgctcggc ttcaaggcgg tcgtaaacct gctcaacggc gtcgagggcc ttgcgttccc
    11821 cggcgtagtt cttggtgccg atcgtgtcgg tccctttgaa ggccatgtgt tcgaacatgt
    11881 gagcgaggcc ggtcctgccg ccgggatcgt tcacagaccc ggcattcacg taggtgtgga
    11941 aggacaccac cggcgcctcg tggcgttcga gcacgatgaa atgcagtccg ttcgggagcg
    12001 tgaattcagt aacccgcttc tccaggtcct gaaggttttg ggcggagaca agcagcgggc
    12061 aaaccaccag caccagcgct gcgccgcgcg taagtcgcat cattcgcatg gatctccagt
    12121 gtcgtgagag ggtaacgctt ccattctacc gtgagcgccg aaatgcgcgg cgtccccgcc
    12181 cactgtcacc gcgtcacggc gccctgcgac aacccatgcg acgaccggcg ctgagcctgc
    12241 tctcctcagc ctgagccgtg tcaccgcaat tacaaaccgt aaccatggtc ttcgacagat
    12301 cctggcgcga tcgatcatct cgaagcgagg agtgggatta ttgactacca gaagagaaca
    12361 cgagcaaaat gacagcatcc tgtcgccgca actccaacct aaactcccca gaaaatcgct
    12421 gcgttcaggc tggtccgttt tggcggtccg ttttggcttg ctcgtcatga tgttttcccc
    12481 tgtgacgccg accgacgcac ctatctcggg ctgctcggtg accagtgtcg actcgttcaa
    12541 ctctcgccta tcggctactg cctcatgtcc aaccactccc aagcacgcac cagctgaggt
    12601 tcagcaggtt ttcagcttct gagcagaccc tggacgatcg aacatcgggt actcaagacc
    12661 gggcaggatg ctggcttata ctatttctct ttcccaacca acgcggcacc tcgtttttgt
    12721 actgaaggta cagcgcgcca aagcgccgtt ctagacctcg ttcctctcgt ggcagaacga
    12781 ggaaattcac caatacgaaa ccgagtacaa atccgaacaa tacaccgacg cttccgaaga
    12841 atattgccca gccgagccag agtcccagct ctgcaacgta catcggattg cgcgtgaacg
    12901 cgtatgggcc gcgcatcatc aggaatgacg gggtcgctat gggtattgaa tgatttactt
    12961 caagcgccag ttccactcgt tccggggttt cagcgatggc aacgaccagg acccagatca
    13021 acattgcgcc gccgaacacg accggagtga gcccgagcag gttccagagg cccgggtgcc
    13081 cgtcagtcca tccgttgcgg ggcatgagcg atgagatggc ccacggcacg acaccgtgtg
    13141 cgatcggaat cagcactaag aagatgacga gcgataagag aaaggacaac cagcgaggga
    13201 cgacgactcc gttcaacggc ttacgctgtg gaaattcggg tgtgcccata gatgcttcgt
    13261 gtcccctgcg cggcagaaac cgcagcggag cgagcccggc ggccccggta tggcagttct
    13321 accggtcgtt ctcccgtcgc gccttgcccc ttacggtccc agcgtgattt cgccgaggtc
    13381 gtaccagccg tcgggctgaa ggccttcaat ggcgaggcgg acagccggct cattcgtccg
    13441 cgggtcgagg accgccacgc gcacgcggta cgtgcccggt ttcagcgagt gatccacgta
    13501 caggctgccg tcgtagacgg cgtcgcccgg catccacttg cggatgtcca ccggaacgcg
    13561 ggcggccgcg gcacccgagg ccgaatggaa ctgcacggcg agatggtgct cgtgatagat
    13621 cggtgacacg ccggcgttca gccaccacat cgagaccggc atcatgtgac cgatgcgcgc
    13681 ccgcttcggg tactgaagct ttcgcagcac gaaacgatag ccgatcttct tctcaaactc
    13741 tgtaaacttc gtgcgccact ccggcgggat ggtaacagac ttcaggttga tcgtcgaggc
    13801 atgccaccgc agcgcctggg cgaggatgta gtcgatgtcc ttgtcgctat acccccactg
    13861 cttccaggac atcgccgtgc cgcaggtttc gagcgacacc ggactgcgtt cccacacgtc
    13921 ttgaatgccc gtgcggacca cttgctgcgg gtacatgtcg agcatgtgca tcatgcccct
    13981 gccccgcccg ccgagatcgc cccagcagtc cagacgccag ccggcgcctt tcccggtgcc
    14041 gtaagcaagc gctgtcggtt cgtcgaagtt catcagcagc ggcgtgcgct tgaaggcctc
    14101 gaaattcgcg tcgatcaggg ccttctgcac ggccatcgac ggcaggtacg gcccccatcc
    14161 ctcgccccag tagccgaccg tcgagacatc gacgctgtcg aggtacggat ggccgtcgta
    14221 acgctggcca gcctccgtta ctagcgcgtt ccagtgcttc gcatagagcg gatcgtcggc
    14281 gtcgggcgac caaatctcgc cgtccttgtc ggtgggcttg ttggcgcggc gcgcgccgga
    14341 gttgcggtac cactcaggca gcggattgcg ctgatcgtac ggcatcaggc ggaacatcaa
    14401 cgtttgccca tgcttgcgtg cttcttcgag cgcgttgtcg atgatgtccc agcggtactt
    14461 gccctgctcc ggttcaagct gcgaccagaa ccagcggaca taggccatcg acgattccgg
    14521 aaaatcgacc ttgcccgagg cgtcggggac ggcggcggtc ggtccgacct cggaccagcg
    14581 catgccgggg tagatttcct gattccggaa acgctggaag gtctggatgc ccatgccggg
    14641 gttcacgagg acggtgtcga cctccttggg ccgcacgacg accgtgtcgg tctgcccaaa
    14701 agcggcaaaa agtgtgcaaa aaagcgacaa aaatgcgctc aaaatgacca atttgggacg
    14761 gtcagagacc atttttccta gactcctttc cgccagcggg gatccgtggc ggacatcaac
    14821 tgcacggtga gagatccgtc ggggttgagc ggctgggcgc acgcccaacg gaccgggcgg
    14881 cgcactccgc ccttggtttc gagttccgcc ttcaggacga cccgggttcc ctcgaggcct
    14941 ttcggtagaa cgaatgaggc ctgccggagc cggccgccgt gcgggtggcc ggcatcgaga
    15001 ccgccgccga ctttgacttt gccatcgggc gtttcgaccg agacgcacag cacgccaggg
    15061 acgccggcaa cgccgtcgtt ggcgaaggca acgacgagct cagtcgtgcc gtatcgtttc
    15121 cgctgccaca cccaagacgg acgcacgcgg tatccgatgc ggcgctgcat cgtttcgaga
    15181 gctttcgggt agcgctcata gaaacggcgg atgttgtccg cctcagtcca gagcgaccaa
    15241 taattgccgc cgaggtcgag gacgtggagc atcgtgtttt cgaggcggct gatgccggcg
    15301 gcgtcggaat cgagcgcttt tggatcgaga ctgtactggc gtttgccgcc gtcctccatg
    15361 atgacggcga gccacggcgg gcggttggag agcgcttcga tctgttccgg ctcgtcgacg
    15421 atgacgctgt cggaacgcag ccagcagccg gcgcgcacgg ccatgtcgat cacttcgcgg
    15481 ttcccggtct tgctgatgtc gggctgtgtg ttgaccgcga gcgtggtgcg cttccatgcg
    15541 tcgagctgaa ggcgcgtcat gccggtgaag actttctcgg cggtgaggta atcggggaac
    15601 gggctcggga ggtcggacgt gtgaccctcg ccccagaagc cgtactgcat gaggtcgacg
    15661 aactctacga gcgggttatt gtcgaactcc gcggcgagca attcattcaa ctcgcggaag
    15721 gctttccgga actcagggtg atcgtagcgg ggctctaaga gatcgtgttc gtagcgccgc
    15781 gcaacgcggc cgatgttggc gagcgggatc ttctcgcgga ggaagtccgg catggagatc
    15841 tgtttgggct ggatgccagg gctcgaaagc tggacgcgga acgcgacgcg gaggccgcgg
    15901 cgtttggcgg cgtcgagggt gagcggccag atgggggcga ggtcgaggcg cccggggcgg
    15961 gactggacgt gccgccagtc gcagcggatg tagaggacgt cgacgaaggg gaggctcgcg
    16021 agcttctcga cgtgccgctc aagagcttcc tcgccgcgac gggcggcgag ggaggggccg
    16081 ccctcctccc atgtgtaacc gacgaggccg agcccgaagt tcttcaccgg ccggtcggag
    16141 gcggtggtgg cgaggacggt gtaccaacct tcgtcctgtt cgatcccgaa gggcccttga
    16201 ttgaggcggt cgtggacttt cggtccgggg ccgaagtcgt agccgtccca acggtgcgtg
    16261 gggatatcag cggaggcgcg tcgagcgaag agggtcgcca ccgcgccagc ggtcttgaag
    16321 aggtcgcgtc tgcgcatgag agtaaggaat gagtatacag gcaaagagga ttgcgggagt
    16381 cagactaatc gggatcgctt tcgtgcagaa cttaccaatg ctagaccgga atcatgccag
    16441 tcatggtcgt aatggtgttc gcgcttgggc tgtccttgac gggctgcgcc caatctgtga
    16501 agactggcga ttgcgggttc tctcggttca atcccctcga agtcccacgt gagtcaatca
    16561 tggacgagaa cacgttccac gaccggcgaa cgcattgggc tgttaccccg gtatgccctc
    16621 cggtggcgcg tgccaagcgc ctaagtggaa cagtccatgt ccgagtgttg gtcgatatga
    16681 gtggtcgcgt cgtcaagaca tgtcccatct acaagccgac tgaacccagg gccgacgaat
    16741 cccttgtcga tgcggcaatt agttacgtcg aaaaggggag atttcgcccg aatcttggct
    16801 cgcccaaggg atcagaagcc ggcttccgct atgccgcgac cacagtcgat attcacttca
    16861 tcttggcaga tcaagagaag tgaaacggtt gggtggcgga agtactgcac agcgatggca
    16921 gtcaagcttg acatcatgat gtctcgattg ggatcatcaa ggtgtgcgaa cgaccttgac
    16981 cctcgatgat gacgtggcca acctcctcaa caaggagatt cagcgttcgg gcgcctcatt
    17041 caagaaagct gtcaatcact ttcttcgtct cggattgatg gcacccaagc agcagaaccg
    17101 gaagcggttc gttgtcactc cccgttcatt gggactccca ccgggcatga gttacgacaa
    17161 cgtggaggag ttgatcgagg cggtggaggg ggccgctcac aagtgatcgt cattgacgcg
    17221 aatatcctcc tctacgcata cgattcgacc tcaacccatc acacgagagc caggcgctgg
    17281 atcgagcgtg ttttgtcggg tggaacaccg gtaggggttc catggcagac tgctacggct
    17341 tttctgcgaa tcgtcacgaa tccgaggctg cccggcgacc ggttcacgct tgacagggcg
    17401 gcgcggatcg tcgatcagtg gtacgaacag ccaaacgtgc gactgctggc gccgggagag
    17461 ggccactggg cgatcctccg gcgagtcatg atcgacggcc aagcgcgtgg tcctctggtg
    17521 accgacgctc aactcgcggc attgaccatt gagtacggcg gcgtactcca cacaaccgac
    17581 cgcgacttcg cccggtttcc cgggttgcgg tggacaaatc cgctggcgta acgctgagtc
    17641 ctgcatcgcg tcgtgcttcc agatccgatc tcatggcgcg tcagacccac ggggtagact
    17701 ataacgttcc ggagctatcg cgagatgacg aatctgaatc gacgggcatt tttgatgacc
    17761 agcggagcgg cgctgggacg ggctgtgttg ccagcccagc aagcggttac ggcggcttcc
    17821 tacgctgaac aatatccgga catgctgctc cggtatcttt cggcaaggct caacgcgcta
    17881 tcggagcgat gggaccggga acgggccgcc atccgcaccg cggaggcggt cgcggagcgt
    17941 aaccgattcg tcaggggcaa gttcgtcgag atgatccacg ggtatcccga aaggaacccg
    18001 ctccgcccgg tggtcgtcaa ggtcctcgag cgcgacggct accgggtgga aaacatcatg
    18061 ttcgagagcc ggccgaactt cttcgtgacc gggaacctgt acgccccgac gtcgggaccc
    18121 ggccctttcc cagccatcct ttcgccgtgc gggcactatc cgctggcgcg catgcagccc
    18181 gactaccagt tcgtctacct gaacctggtg aaggccggct tcgtcgtgct gggatatgac
    18241 cccatcgggc agggtgagcg gcggtattac tgggacccgc gaaccgatga aacggagtct
    18301 gcgctgggtc cggtctacga gcacagcatg ccgggccaac tgctgctgct cctcggcgaa
    18361 aacctctcgg cgtatcgaat ctgggacggc atgcgaggaa tcgattacct gctcacgcgg
    18421 ccggaggtcg acaaggagaa gatcggctgc gccggccact ccggaggtgg caccctcacc
    18481 ctgttcatca gcgcgctcga cgagcgcgtt cgctgcgccg tggtgaacga gggcggcacc
    18541 ggtcaccgct ggccggtgca catcgcccct ggggggccgt ttgggccgtc cgacgtggag
    18601 cagaacctgt tcccgtccgc gctctacgga gcggaccttt gcgatctgca cgttgccatc
    18661 gctcctcgtc cactgcttac gctgaccgaa gaatactcac cgtggttcaa cctcgcggcc
    18721 ggccacatcc gcgatcgcta ccggcagttt ggtgttgcgg agaagttcgc gacagaggag
    18781 gccaccgacc ctcacgccta cacggtaagg ctgcgccaag ccaccaccga ctggttctcg
    18841 cactggtttt atggccgcaa aggccctgag gccgaacccg atttcgacgc cgaaccgcgc
    18901 cgcaacctgt attgcacgcc aaacggatcg gtccgttact cgcagcaagg cgacacaatc
    18961 ttctccctga ttctcaagag gcaggcatcg ctaccggagg aatccaagcc gccttcgtcg
    19021 cgccgggaat gggaggagtg gcggggaagg ttcctgccca cactcaagga actgctgcgc
    19081 tacaagccag tcacgggacc gctgggcgtc cgcccactcg tgacaacggc gcgcaaaggg
    19141 taccgggtcg agaaactgga gttcctctcg gagcctgaca tctacatccc cacctgggtt
    19201 ttcctgccgc aaacccgaaa tgccgagacg cgggcgattt tgtacctgaa cgacgccggc
    19261 atcgccggag agggtttgga agggatggaa ttcggcgtac tggaagagct ggcgaggaag
    19321 ggccatctcg tcgtggcggt ggagccacgc ggaaccgggc tgaccgcgcc agagcattcc
    19381 gagggagttt cggaagcccc ggaattccgg cagttattcg acaccgaaac cgccgcggcg
    19441 tacatggcgt ggttcatgga tgagtcgctg cttgggatgc gtgtgttcga cgtgatacgg
    19501 agcgtagatt atgcgctcac acgcaacgat gtggacaagc gtggcctgcg cgcgatcggg
    19561 acgggaagag gcgcgacctg gctgctattc gcggcggcgc tggacccgcg catccaggct
    19621 accgtttgcg accgcggcct gctgtcgtat aagtcccttg cccagagcga ccggtatctc
    19681 tatggcgcgg atgtcttcat tccgggggtt ctgaagcggc ttgaccttcc acaggtagcg
    19741 gcagccgttg cggaccgatc actgacactg ctctctccgc tcgatccgat gaagaagtta
    19801 gtggacgttt ctgaagctac cgtcgcctat gacaggaccc gccgaactta cacgggcgct
    19861 caagtcggcg gccaattccg cattctgcgg cgcgagccgg aactgtcttt atccgaccaa
    19921 tatctacacg cgttcgaaac ctgaacggag gtcttttgta atgtgggccg attacgccgt
    19981 tggcgggtcg gcggcctcca ccagagtccg cgaaacaggc gcgcccacgc aaatatgaat
    20041 cgcatggctc tcaccgtcgt cgaccaactg gatctcctgt tcagaccgtt caccgccgtt
    20101 ttccacctcg atccgatact cagttcgtcc gtagcggaag gtcaggacgt aacctggcca
    20161 gtcctcagga atcacgggat cgattgacag acgtccggct cgcagcttga acccgaggac
    20221 ttcctccagc cacacccgat acatccagcc cgccgagccc gtgtaccacg tccaacctcc
    20281 acgcccagtc tgactctgca aggagtaaac atccgcggcc acgacgtaag gctccgttcg
    20341 ataggccgca taatcctccg gtgtgcgtga atgctcgatg ggattcagca tctgcagaac
    20401 tttgacggca cgatcgccgt ctctcatgcg cgcaaacgcc attgcaaccc ataaagcggc
    20461 atgtgtgtat tggccgccat tctcgcgcac acccggcgga taccccatga tgtagccggg
    20521 gtgggggctg gatttgtcga agggcggcgt gcaaagcagc acgagtttct ctttctcacg
    20581 gatgagatgt tcttcgaccg agcgcatcgc ctccgccgcc cgatccggcc tgccggcgcc
    20641 actgatcacg gaccacgact gcgatagcga atcgatgcgg gcttcctcat tccgccgcga
    20701 gcccagcggc gtcccgtcgt cgaagaaggc tcgcagatac cattcaccat cccaacttgt
    20761 gcgttccacc gtcgcactca agcggtccgc gcggccgcgg taacgggagg ccaactcctg
    20821 ttcgccgcgg cctgcgcata cctcggcgaa cttcttcaag acgtccacga ggaaccacgc
    20881 cagccagacg ctctcgcctt tcccctcatc gccgacgctg ctcaggccat cattccagtc
    20941 gcacatgcct atcaggggca acccatgcgg cccggaggtg acgcccttct caatagcccg
    21001 gcggcagtgc tcgaatatgg tcacctgatc gatcgagacc gacggctgaa aatagacctc
    21061 gcgctcctcc tcttttagca gcggtccctc gagaaacgtt gtagcggcat tgagaatgcc
    21121 agcgtcgccg gtgacatcca tgtattgaca cactgcgaac gggagccaca aaagatcgtc
    21181 cgaacaccgg gtgcgaacgc ccgctcccga cgggagatgc caccaatgct gcacgtctcc
    21241 ttcggtgaac tgccgcgaag cggcgcgcaa gatcagctcg cgcgcaatcg gcgccgcgga
    21301 gtaaacaaga gccagcgcat cctgcaactg gtctcgaaag ccatacgcgc cgctcgattg
    21361 atacagagcg gaacgtcccc aaatgcggca actcagcgac tgatagagca gccagcgatt
    21421 caacatgaaa tcaaccgata gaatcggcgt tttgacctga atcgtggtca ggagatcatc
    21481 ccaccactcc cgcgtctcgt tgagcgaccg ttcaacgttt tccgcgtcac ggtaccgcgc
    21541 aatcaaacgc ccggcgtgtt cggcatcatc cgcctgccca agtacataag tcactgtctt
    21601 ttcttccccg ggaccgaggt cgaatttcgt ttgcagtgcc ccgcacggat cgagcccggg
    21661 acccgtgcgc ttggaaagcg actccagccg caaagccgct ggggcttcca ccgagccgtt
    21721 gcgcccaagg aatgcagtgc gatcaccggt gtatgaagag gcctccggtg tcatggccgc
    21781 gaatgcgacc cgccctccat aatccaaatg atagggattg cgcgaaaaca acgctttcgc
    21841 actctcatcc caggaggacg ccacatgcat ctgcgtagcc tcacgatccg tgccgagcac
    21901 gagctccgaa tatgaggtca cggacagacg gcgcctctgc gaagaattgt tcctgacccg
    21961 cagacgctgt atgcgaaccg gatcggctct ctcggcgtgt accggcacaa aagtcaccag
    22021 cgtttgttcc aacgcatgac tggtgtgttc gtgttctgtg tatccctgcc cgtgccgggc
    22081 acgatacgca tcgagttcgc ggaccggcag cggtgtaggt gtccaaaaca ctccgctgtc
    22141 ttcatcgcgg atatagatgg cctcggcaga agggtctgaa accggatcgt tgctccaagg
    22201 cgtcaaacgg ttcgactggc tgttgccgta ccagcagcac cctgaaccgg cttcgctcac
    22261 gagggctccg aacaccgaat tcgccatcac attgatccag ggcagcggcg tgaacgcatt
    22321 tggcccgagg tagatcgcat attcgcgccc atccgcggta aatccgccaa gaccgttgaa
    22381 ataggcaagc tccaggaagg gcaaaggcgc agagggttgt tcctcacgtc gcctgatccg
    22441 caacggcggc ggcagagaaa ttccctccgg aggactgcta agctgcttgg acagcgttcc
    22501 gcgcaccgtg cccactgtcg cctgcgccac ggtgaggatc aaattgagat cctcctcgga
    22561 tatctgatcc gccttacgaa ggaacacgcc gcctggctgc tgcaccccgg tgtgcaatga
    22621 gtgcgcttcc accaggcgga gcaactgctg atggagaggc tgctcatagc tagccggctc
    22681 gcggttgaga actaagagat cggctttcaa gccgcgcagc cgccagtacg tatgtgcaac
    22741 cagcagctcg cgcaccagag taaggccttg cgaatcgccg acccaaaccg ccgcaagcgg
    22801 caagtcgccg gagataccgt gagaccagag cttcgactga gtttgcacat tccggcgcag
    22861 gcgttcggcc ggcgctcgca accggacgtt cggatacagg aggtgaccgg cgagttcggc
    22921 aaaacgcatc gccgcatcgc tctgaatacc gagatagcgg tattcgagtt gcgcgtgtga
    22981 ccaggccagc tcgaacgcgc ggctgcagac atcggaatcg cggtacttga ccaccagacg
    23041 catcaggtcc tcgcgcgacc ccgcggcgac cgtaaccagc gtgatctgcg cctgctgcct
    23101 cggttcaagc gaaacgcgct gtctcatgac gaacacagga tcaagcacgt agccatcggt
    23161 ctgtttcagc gacattgccg ggtcgctcca ggtgcgtccc cgcccaagga accgcgcgcg
    23221 gtccgtttcg tactggaacg agctctgatc cttggggctt tccatcatca actgtgcaac
    23281 ccagatcggc gattcatcgg gagaccgtag ccggcgccat gcaagtagcg cgtgcagatc
    23341 gggccgcgcc tcggtctgga taaacagctt gttaaaagcc ggatgggcac ggtccgcgcc
    23401 atggggcgca aggctcaatt cagcgacgct ggtcaactcg agcgtcctgg ctcgcgaacc
    23461 gtgattgacg agtgtgatgc ggcgaatctc cgcatcatct tccggcgata ccgtcacttc
    23521 cagatgactc tcgataccca gcctgcggcg acggaactcg gcccggtcgg cgctgaaggt
    23581 cgccgtgtag cgggggtctt tcacgtttaa gggctggtgc gtcacggacc aaagcgtgtt
    23641 gctttcttct tcgcgaagat aaaaaaaagt tccccaattg tcacgggtgg tgtccgaacg
    23701 ccagcgcgtg atgtcgaact cgccccaacg gctgtagcca gcgcccgtgt tcgtgatcat
    23761 cagcgcatat cggccgttgc cgagcagttg gacttggggg atcggagtgt cttcatcgaa
    23821 cacccggtat tcaggcgccg atggaccgga gatcagccgc attgcgacct gatccgatgg
    23881 gcggtgaacg agcatcgagg gcaccggcgg tatgcgctcg aacaacaacg gctccacggc
    23941 tttgattcgg cgatcggcat ggaagcgctg ccgcatgatg ccgcggttga gcacgttgtt
    24001 cagagccacg aggctcattc cctggtggtg agccatgtat gcgtaaacga ttacgccctt
    24061 cccgcctctc cgttcctcct gacgggtgta gtccagcgac tcatagaaac ccatgcggcc
    24121 gtacattccg gccttcacca gccgcttgag gttcttgatc gattccgccg ggttcaccag
    24181 cagagcgagc gcggtggcat agggcgctat caccaagtct tcctccagcc cccgcttcag
    24241 gccgagcgat ggaacgccga aagctcgata ctggtagatc ttgtgcgaat cgagcgcgct
    24301 gtaggcagat tccgacattc cccaaggcac gccccgatcc tttgtgtact caatctggcg
    24361 tttgaccgcc acggcgcaag cattctccag caaggagttt cggaaactgc gcgtgaacag
    24421 gagcggcatc aagaactcaa acatcgtgcc gctccaggaa agcagaacct ggccgtccgc
    24481 cgaggtgtag ggccgtccga gcgcgagcca gtgctccacc ggcacatcgc ccttggcaat
    24541 cgccaccaga ctggcaagac gcgcttcgct cgccagcaaa tcgtagtgag cggagaggtt
    24601 tagtgggccg cctacctggt atccgatagc aaacagacgc cgatgagcgt cgtacaaaaa
    24661 ctgcatatcc atgccgtcag cgagggcgcc gcttgtttct ccgagatgtc tggctcggtc
    24721 gaggagatcc gccgacgctt tacgagcctt ctcgtactca gctccgacgt ccgcaatcca
    24781 ggcggtgagg ttcgccgaga gcccgtcttt gttcccggcg ctcttcaaga tctcgctcag
    24841 caggccggtt tctccgcgag ccaccgcgcc ccaggaaggc aactcgcgca gcagcctacg
    24901 gcgcgccgca atcgcgctat ctccgagagg ctgaaggaac tcgtcaggtg gagcggccag
    24961 aacgtcgagc cagcgcaaat aaaggtcggc gtgttgaatc cagctctcta tttgccgctc
    25021 cagccgcgta aaccagtaag cgggctcccc ggtcgtcgag acgctccacc tcagggaatc
    25081 ggtcagcttg cgtgcgggtt cggcagcgag gcggattcgc tccagaattt cgatgcccga
    25141 agactcttcc tcgaacagcc cgcgcaacgc agccagggaa acacggttgc tgtggtccgg
    25201 ctgaaaccgc tccgtaataa ctgctaacgt gtcagccaag ccgcgcagtg cgcgcccgtc
    25261 caattgcgat tccgcatcca gctcctgaga ggcctgctgc agaacccaca ggctcgcgat
    25321 gaggtttccg ctatccgcag tcgatacgta ctttggcggc agcggttcca gagtccgggt
    25381 gttgtaccag ttgagaaggt ggccctcgca ggattccagc tttgccagcg tttcgatcgt
    25441 cgccgtacac cggtcgatca ttggctccgg agtcagaaag ccgaggtcgt gcgccgaaac
    25501 cgccgatacc agccacattc cgatatttgt cggcgaagtg cgatcagcga cctcgacgcg
    25561 caatgcctcc tgggaattat ccggtggaag ccagttgtgc tccggtccta ccaagtcatc
    25621 gaaatagcgc caagtttcac gggcgatgcg gcgcagaaac cgctggtcgt tgagttcgat
    25681 ctgttccgct ttcctgagcg cgcgtcgttg cgacctgatc caatgctgga ccgccggagc
    25741 ggcaacccag agcaccagaa acggagccca agccggttcc caaagacgcc cacggagcgc
    25801 cagggacagc atgaaacccg cggtgacggc gcagatcagg aagaaatgta tacggaaggg
    25861 atccaggtgc agacgggcgg catggtacga catttccgca gtctgccact ccagcagacg
    25921 gcgtttgctg atcccgagcc gatacaagac gcgaacaatg gcatcggcgg agaggtaggc
    25981 ctgatgcggc aggaaggcag ccttgacgat ggcgagattc aaatcactgt tggcttcgcg
    26041 ccaggcccac gcgtctcccc gccatcgcgc gaccaggcgg tcgagaaact gaaagagaag
    26101 aggaacaacg agggtcaacg ccactagtgc gctggcggcg gcgggagcgg cgtggaaggt
    26161 ccaactgaac actaacagca gcaacgacgc caccggcacc aagctgcggc gcaggttgtc
    26221 gagaatcttc cagcggttga tcagcgtcag gggattccgc gcgtcctccg gccgagttgc
    26281 ggccggaact cgcggcaaaa tccatgaggc gatctgccag tctccgcgaa tccatcgatg
    26341 ctgccgttta ctgtagctcg tgtaatcgta tgggaactgt tcgaagagtt ccacatccgt
    26401 ggccaatccg acgcctacat acgctccttc gatcaggtcg tgactcagca acctctgctc
    26461 cgggaagcgc ccgttgagca ctttgtgaaa ggcacgaaca tcgtaaacgg cttttccatg
    26521 gtagatggct tcgccgaaga ggtcttgata gacgtcggag accgcctgac aatacgggtc
    26581 cgtcccgcgc gcgtccgtaa agagtctcgt gaaacgtgtg gccgtcgccg acgggagcgt
    26641 gatactcaca cgcggctgaa tgatggtgta accgcgagcg tggttgcgct cgtccgcggt
    26701 tagctccaca cggtttaaag gatgggcgat ggtttcgacg agcttttgcg cactgccgtg
    26761 agggagttcc gtgtcggcat cgagcgtgat cacgtatcgg atcccctccg gcagcgaccc
    26821 gacgcacagt aactcagtgt tcttcccgcc attcagaaaa ctgttgagtt cctcgagttt
    26881 cccccgtttc cgttcccaac cgatccagcg gccctcggtt tcgcaccaca tccgcggtct
    26941 ctggaagagc aggaagcgcc catggccgtg acgtgaattg agctcctcta tgctcttcat
    27001 cgccaatcca agcagaccgt cgtcctccgg catctcgcgc tcttcagcgt cagtgaagtc
    27061 cgacagaagc gagaaccaca gattcgagtc cgggttcgcg aggtagcgta cttccagctt
    27121 ctcgatctcg ccccggaccg actccggtgt aagcagcatc atcggtacaa cgacaagagt
    27181 gcggcagtct tccggtatcc cctcctcaaa cgacatcttc ggtagcacac gcgggggaag
    27241 aatcgaagta aggaacatct gcagcaggta agtgacgagc tcgctggctg ggaagagtgc
    27301 caatattccc agaaacagga gaataccttg cgaagttacc cccgcgctgt acgccgcgaa
    27361 ccgaaaagca cccgaaagtc cggccgttag cgcggcgaga ctactcagat agaaaaaggc
    27421 cggatatcgg tgcagaaagc gccgccacgc ttcgctccag cgaatgcgac ggcgcaggcg
    27481 cttttcaagt tccggcaatc cttcgtcaag aaggtagtaa gctacgcacc gctcacggct
    27541 gccggggggg gcgggttgcg cgaggtccac cgccaggcga gcaacgtcac attcggaaaa
    27601 cttcgactgg cgcgctgccg cttcgacaac ccgccggcaa cggtcgcgcg tggcgaaatc
    27661 gctgcgggca tgaacgcctg ccgggtcctg gcgcagaatc tcctccatcc ggctcaccgc
    27721 ctcgacaatc tttgggtact gcagttcgga caactggcgc aggctcccaa tcgcgctgga
    27781 gatcaacatc agatcgttcg cttcttcggc gtgctcgccc cggatgatgt cggccaagcg
    27841 atgttccgtc ttctcctgaa tccatttctg gatcggagca agcgcggact catcccggtg
    27901 cagttgctcg ccgaggcgcg caatgaaatg gggcgttagc tcgttgccat ggcgatcgag
    27961 ttcaccaacc atttcctcga actgctcggg agtgcgatgg gccgcgttca gaaggcggtc
    28021 ggcccagaag tcggccagtt ccttcatatg ttggcgtaag atcgccaact cggccagacg
    28081 ccgcagccgc tcaagaagca ccagccgtaa catcagcggg aacacccaca gttcggcgac
    28141 agtcaacgga gactgatgtt gataggcgtc gagaaagcta atgatggtct ccgtcgcgag
    28201 ccggtggccg gtgcgatcga tcaattcagc ggcgagatga tagacgcgca gccggaccgg
    28261 acagctccgg tcggttagca ccggtaagat cttgttgtga ttgtcaggaa ggttgtgccg
    28321 gatgtcagca atgtttgagc gaatgaggta tgcgttatcc agtagccact cggctgcatg
    28381 cgtgatgcca tattccagcc gcgcggcttc ggccagatcg gcccgagcgc gttcgatgac
    28441 gacctcgcac tcccgcaaaa tcggcaacag cggccgcgac cgggtcgacg cctcgagttc
    28501 gtgtgaagcc gccaactccg ccgcgcaatc cggcaactcc gccatggtgg ccggttcgcg
    28561 ccgttgtccg gccgtctccg tcgaggcagt gaagccaggc cctggacgaa tcaccaagac
    28621 ggatggatga ccgatgcgac ggaggaccgc tacgacatcg gccgcctcat cttcagttgc
    28681 ttcgactacc acgaggctct ctccgggaag gacaaagccg cgatagccac gaaggactct
    28741 gtcgctcatc cccagtccca accagagcgt gccaaaccac gtgatcagaa agccgcacaa
    28801 cgccgctaaa actgccatca agggaggaat gcccgcgatc gtggctagca tggcgacggc
    28861 gagcgccaag cctacgccga aggcggcccg gttacggggc ccgagtccgt catggaaatg
    28921 tttcagaccg ccgtctgacg cgcgatggat tacagccgag cgcctgaacc cattcgtgcg
    28981 gacctcgcga agcgcctcct caccgtgctc ttcgctgcgg tagaacccaa gcacgaatct
    29041 ggagacgcgt gatttcctag cacacgccga aaaaagcaca tactcattgt cgccgtttct
    29101 ttgacgctcc tccactgcag aactcgacat gtcccgatac gccctccccg acttcttttc
    29161 cgcggaggtt tcggcccggc gatgccgttc tcaaaactgg aacagggtgt cgcgacgggc
    29221 tggatgagcc gtcaaggtat tgaaaggagg gtcagatagc ctccgaagtt acattacact
    29281 acgtttcggc ggagaggcgg gcgcggacga ttcgtccaaa cgaggtggcg gtgtatcttt
    29341 cgacgatgag gggggcgttg tgccagctcc aggccgccca gcccatctgc aggctgtcga
    29401 aatagtcgag gagcttccgg ccccatttga gatcactttc ggcgccaccc cattccccgg
    29461 cgaacacggg cactgtcgcc gcaagatggc cgaaggcctc gtgccagttc cggcccttga
    29521 tcggatagac atgggtggaa tagaccaggc gcggaatgtc catcggcata cccctaaggt
    29581 catatgccca gttggtgcca ccgacaaaaa tcagagaatc ggggttgaca tcgcgaatcg
    29641 cggcgatcag tttgcgcgcc cacggctgcc actccgccat cgtgacgcgg tacaggccgg
    29701 gcgggtaggt tgtcccgtct tcccggttga gggggtacgg gtcgtcggga agccgatcgt
    29761 gcggctcatt gaagatgtcg taaagtacgg ccggctcgcc ccggtaacga gtggcgagca
    29821 aatcccagag ttcgatggac tgcatattcg gcaatggggc gacgaagttc ctgtcgccgc
    29881 cataggcacg atccgcatcc agccactgca agtccagaag agtataagcg ccgaatccgg
    29941 acgcccagcg gatcatttga tccaacgcct ggcaatatgc gtcagccgtc caaccgcccc
    30001 gcccgcgcaa aacccagtcc tggttaaagg gcagacggat tacgtttgcg ccccactctt
    30061 ggacgatcgt tcggatttca ttgcgggaaa tttccgcggc ggagaggaag ccttgctcgt
    30121 ccggttcaga gtattccagt ccggaccggt tcaggccgcg caaaagaaca gtctccctcg
    30181 tgccggctct gacgattcgg ttgccctcgg ttgtcaagaa cggcagcccg ttcgagaggt
    30241 caccatccat ggcttgatcg tacatcggcg gactcggtgc gtataggcga ggacgcggtc
    30301 gggcgccgct ccctcacagg cgttgtttat ggaccgctcc ctggggtcac ggctcgctaa
    30361 ctgagtccga aatacaggcc ctg
//
LOCUS       DATIFQ010000542        24229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2485839, whole genome shotgun sequence.
ACCESSION   DATIFQ010000542 DATIFQ010000000
VERSION     DATIFQ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 24229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24229
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2485839"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VLE22_27295"
     CDS             <1..1416
                     /locus_tag="VLE22_27295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18189.1"
                     /translation="RNGDKEFPSGSYVIPLAQPAKRLVRTLLDPNIPMEEHFVKEQER
                     RRKKNLPDEIYDVTAWNLALMFNVECIPASEASQGSFTPAGDRTPPGAVAGDNATVAY
                     LAPWGTLASGRLLAASLRSGLRVWSADKPFTLGGRKYPSGTLIFKVKENAASLAEALA
                     KLAASTGAEIVGINSSWVDGGISLGSNHVVPMRKPAIALAWDTPASPLSAGWTRYVLE
                     RQYDYPVTPVRTRQLASSGLHRFDVVILPEPSGSYMDTLGAAGVKRLKDWVTDGGTLV
                     ALGGSVAFLADSKVGLLSTSRENLAKPVEPGKPPEQKETAPAKPDAQAPARVPGKLLT
                     SETEYEKALQPEAEPPDSVAGVILKARVDSDHWLGAGLSGTVNAVVVGREIFTPIKLD
                     KGVNVATFLGPDQLLQSGYLWEENRKQFAYKPLAVAQPDGRGVVIGFTADPNFRAYVD
                     GLNVLFLNAVFRGPAHARPAP"
     gene            complement(1417..2037)
                     /gene="ribA"
                     /locus_tag="VLE22_27300"
     CDS             complement(1417..2037)
                     /gene="ribA"
                     /locus_tag="VLE22_27300"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HSB18190.1"
                     /translation="MENPAVAARLRKVAEADLPTSFGLFRIFGFKGEYGGRLEEAVVL
                     RMGEVSGDPPPLVRIHSQCLTGDVFHSLRCDCRAQLELSLARIAGEGRGLLVYEHKEG
                     RGIGLLNKLRAYELQDHGADTVEANEQLGFEADLRNYELPAEILRHFGLTSVRLLSNN
                     PDKIQALERAGVEVSERIPCIIAPSDTTEIYLRTKKEKLGHLLEGF"
     gene            2102..3412
                     /locus_tag="VLE22_27305"
     CDS             2102..3412
                     /locus_tag="VLE22_27305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSB18191.1"
                     /translation="MLDQHLIRLLVKLGIVASLASILVRSDAFKRMLLREQRTLNQRL
                     LLALSFSSIFATGVATRVVTRSYQAVDLGLEGSLLSGILGGYVTGLVSGILISVPAMF
                     NGEYLSMPLLAGVGVLGGLLRDCAPDTEDIWHFSPFFDLSIYRLFRRGHDHRRSAFHL
                     FFLLAILFAEFLRITLGHIFKDSVFYLYPEWVSPHPLTIVIVYITTLFAVTLPLKIWN
                     NTRNESKLAEQQQVLLRARLEALSSQINPHFLFNTLNSVSSLIRTDPGEARNVVYKLS
                     NILRRLLRKHDNFNPLRDELNFINDYLAIEMVRFGDKLHFEKDIAPNTLDRPVPSMLL
                     QPLVENCIRHGLSGKVEGGTIRVSSQLADGRLHLSVEDDGVGIPEVKLATLLEQGIGV
                     SNVNERLKVLFGNDYRMFIDSKPGTGTRIEIQIPELQLPGTGVS"
     gene            3434..4120
                     /locus_tag="VLE22_27310"
     CDS             3434..4120
                     /locus_tag="VLE22_27310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSB18192.1"
                     /translation="MTNLQTPVLIPETNGAHRTIAGRTRRVYDRLAAVYPVSTLFFHS
                     TAHKRALAVAGLVDGMRVLEAATGSGEMFRRLVRANRGGETVGFDLSPNMAARTHRRV
                     RREFPAATTLCQAVDARYLPFCGESFDLVVCCYLLELLSSEDILLALSEIHRVLRPGG
                     RLALVAIGQNTEFFNRVYRVLGKIAPAFWGRQIDGRLIELIETADFRLLSEEHVRQSL
                     YPSRVLLARK"
     gene            4166..5365
                     /locus_tag="VLE22_27315"
     CDS             4166..5365
                     /locus_tag="VLE22_27315"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB18193.1"
                     /translation="MQSDRRQFLKNSLGVSAAVSAAPMIVPRRAWGANDKPAYALIAA
                     GGRGRYLNKVFKKLGAQCVAVCDVYEPYVEMALEDSPDARAYVDYKELLEKEKSADFV
                     ILAGPDHHHCPMLLASLAAGKDVYAEKPLSRTLEESQKMIQAVKQSQKVVQIGMQRRS
                     APVIRSAKRLIDDGVLGKLAMVKPMWNWNVSGPIDNSPLPGKLDWQRFLGDAPQRELE
                     PMRFRRWRYFWDYAGGNMTDQGTHLMDVVLWFTNSGPPVSAICNGQVNKMKGAEHPEV
                     FCAVFEFPSFLVTWTLNYCNSYENGWSITFMGDEGTMILDEDGYRVYQEPWALPKNRE
                     PIFYAKVAVPIEAHVQNFLDCIKTREQPTCDVESAAKAVAGPHLANIAFQQGRKATLA
                     PDMVTVS"
     gene            complement(5369..6145)
                     /locus_tag="VLE22_27320"
     CDS             complement(5369..6145)
                     /locus_tag="VLE22_27320"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HSB18194.1"
                     /translation="MPDNLVIAGREFRSRLIVGTGKYRSFQEMARCHAASGAEMVTVA
                     VRRVNLTDKSKESLLDYIDRSKIFILPNTAGCYSADEAVRTARLAREVGLSNWIKLEV
                     IGDEKTLFPDNEGLLEATRILVKEGFVVMPYTNDDLIYAKKLIDAGAAAVMPLAAPIG
                     SGMGIQNPANLRILREMITAVPMIVDAGVGTASDAAVAMELGADGVLMNTAIAGAQDA
                     EKMAVAMKLAVEAGRLAWLAGRIPKKLYASASSPLEGVVR"
     gene            6179..7564
                     /locus_tag="VLE22_27325"
     CDS             6179..7564
                     /locus_tag="VLE22_27325"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HSB18195.1"
                     /translation="MKRTLLLLLFVLPLAAGDFRLGQAAVKITPPAGAPMAGYYHNRA
                     ASGVHDDLFAKTLVLEKDGEKTALVACDIIGMPRGVAEEARRLAEKETGIPGARIMIS
                     GTHAHTGPVLYEEGSRYNLEGDMLRIAREYIAALPAKIAESIKLADSRLQPAKAAAGV
                     GHEESISFNRRFFMKDGTVGWNPGKLNPNIVRPVGPIDPDVGVVRFQAEDGDSLATYV
                     NFALHLDTVGGVEISADYPYTLSKLLGRLNGADMLTVFTIGAAGNLNHINVRTAARQN
                     GHGEAERIGGVLAGEVLKTYTRLEDIAATSVRSRSEIVRLPLAEHEPSDVEWARGVAP
                     KFGKPGAAPFLDLVKAFRVLDVEARKGKPIDAEVQVIALGDQVAWVGLPGEIFTELGM
                     AIKRASPFPYTMVAELANGSIGYVPNQESYSQGAYEVVSARCAAGSGEMLVDTALRLL
                     KQLKATGAATN"
     gene            complement(7568..8494)
                     /locus_tag="VLE22_27330"
     CDS             complement(7568..8494)
                     /locus_tag="VLE22_27330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HSB18196.1"
                     /translation="MKPLGVIVLPILLIAQEPNPTQQVNVNPAQPPAPGSAPIYRVNV
                     LSRTTKAISYRPDGDSTKIGFQGTVLLPRAEGQARVKGHKGAFGIEAEFRGLEPPHRF
                     GPNYLTYVLWAITPEGRSTNLGEVITDHDNRGKLRTSTELQSFALILTAEPYFAATHP
                     SDVVVLENTVLPGTRGNVQQVEAKYELLKRGEYTLDLQDARSRVAAADGPKLSLDRYE
                     ALLEVYQARNAVQLAAAQGAEEHAPGALRKAQVQLAEAERLFSQKAGRSVIVEAARGA
                     TQTAEDARLIALRRQRTDTPPPNAKLGTGSLQ"
     gene            complement(8605..8907)
                     /locus_tag="VLE22_27335"
     CDS             complement(8605..8907)
                     /locus_tag="VLE22_27335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18197.1"
                     /translation="MRVLSTLALLLALTAGLALADQWVGWVTDEKCAQTGTFTGEKHK
                     NCINSGGTVVFVNEADKKIYHLSDAEKAKKFVGQKVTMQGSAKDDTIEVESVTPAT"
     gene            9239..9315
                     /locus_tag="VLE22_27340"
     tRNA            9239..9315
                     /locus_tag="VLE22_27340"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9273..9275,aa:Ile,seq:cat)
     gene            complement(9496..9909)
                     /locus_tag="VLE22_27345"
     CDS             complement(9496..9909)
                     /locus_tag="VLE22_27345"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HSB18198.1"
                     /translation="MRADEASKFAKAEELLNLTQTGQMMKSFLEPMKGVLTAEMNKTE
                     MPAEKRAKASEMQGKIMALIADRLSKAKPELVKIYTDTYTEEEIDGILAFYKSPAGKA
                     LLQKMPQVMQRSMPVMMQLMGDLQPEIKKMTEELK"
     gene            complement(10032..10502)
                     /locus_tag="VLE22_27350"
     CDS             complement(10032..10502)
                     /locus_tag="VLE22_27350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18199.1"
                     /translation="MASVNTVPEGESAKARNAANALLVALLLIAVLCVLVWWWHQSTP
                     PGLSDVRETVQPPRTAKPPEVAAPATRPMRKEEPQRAEKPKQPAFPTAESVPVGMEKS
                     ELLARFGKPNMVTTSVEQGQEMETLVYLRRGPPKKETVLVLRNGSVVSVNSVNP"
     gene            complement(10659..11051)
                     /locus_tag="VLE22_27355"
     CDS             complement(10659..11051)
                     /locus_tag="VLE22_27355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18200.1"
                     /translation="MTVERAARPFQICRSCRHEWSTWDRFVLDPDLRLLGLQAVITHP
                     DVNLLVFEHGCGSSVSILSRRLRHLLPEPEPDASRVRLLGSEQCRGHCLRLGDLEACD
                     APCSNARDRELILLVQRMRREARESCPQ"
     gene            complement(11246..11530)
                     /locus_tag="VLE22_27360"
     CDS             complement(11246..11530)
                     /locus_tag="VLE22_27360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18201.1"
                     /translation="MVNGKAASKDSAGASVALLGRSRVFFPSPFRFGHLTEDALCLYG
                     INRLPDADVPSVLKHLLECECCFDRMLGIVTLAFVRMLHRTTGALYGTNG"
     gene            complement(11628..12425)
                     /locus_tag="VLE22_27365"
     CDS             complement(11628..12425)
                     /locus_tag="VLE22_27365"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HSB18202.1"
                     /translation="MLGADTEILLFPVMSAISTVLVAAAFFFPLYFAGTLLAIKDGAG
                     GWADYAVLFVWYYLNYFVVIFFNSALVACANIRLSGGNPTVAEGLRVAMSRITRIAAW
                     AFVAATVGIVLSWLRDRGNRLVRLLSSGLAVGWALITYLIIPVIIIENRNVSDGMLRS
                     SELFRKHWGEQVAGSFGFGLLNLLLLVPGLLLGWLVYPYDLGAAIILTMVYILLLAAV
                     SSAVKGIFTVALYRFATDGVAPYGFDADSIDPNWRYHRRLNDPGRSS"
     gene            complement(12481..13953)
                     /locus_tag="VLE22_27370"
     CDS             complement(12481..13953)
                     /locus_tag="VLE22_27370"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSB18203.1"
                     /translation="MNSHFSFFDWVLVLGFLAFYAYLGIRARAHSSSVDEFLVMGRRL
                     GPVWGVATLAATETGLITLIYFAEEAYLSGFVALIVALLAAVTMFFVGWTGLIVRKLR
                     ELEIRTVPEYMESRFNTRVRTIAGLATFIVGVLNMGIFLQAESNFMAIIMGIPESRIL
                     LVMAVMLVVVVAYTMLGGMYSVVLTDVVQFIAIVAGVGITTLLIFSNAGGWGGMAEAV
                     DRRYGDAGFYIWKAPRYGPLFLSWTLLYYLSGWSSWQPVVARILSMRDIATALKLYRI
                     SSLFMFMRAAFPMVWGIGALAILGRTGESSMALPQMLARIVPAGLIGIVTIGFLSASM
                     STYSSYFLAFSSILFQDVIGPHTKRLPGGKGRVKAVQAGVLVIGAFIYIWGSFYHFPE
                     SVFRYITLSGSLSFAATLTVLIGGIYWKRANVWGAYCAFLGSAIPPITCLAIPSIDPT
                     YAGLLSFTLAPAGLILGSLAFGRTSTRLLSSSGGDSMARG"
     gene            complement(13950..14618)
                     /locus_tag="VLE22_27375"
     CDS             complement(13950..14618)
                     /locus_tag="VLE22_27375"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB18204.1"
                     /translation="MVDFHNRWNLPFVNIRRMLQSGEMGDLLAINIRLNDTLFVPTKM
                     LGWASCSSPAHFLGSHVVDIIRWLSGAEVTRVYSVSRSLVLKNAGIDTPDFFQSILEL
                     SSGATAVVENCWVVNESSPSVFDFQCEFIGSAASAYVNASHHAMLKTYTTGGVAYPDV
                     ACAADLHGKPVGFGVAAIEHFIDCLTSGREPMVTGEDGLAAARVVAAMVKSAGTGQPV
                     ELRQ"
     gene            complement(14619..14801)
                     /locus_tag="VLE22_27380"
                     /pseudo
     CDS             complement(14619..14801)
                     /locus_tag="VLE22_27380"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            15103..16320
                     /locus_tag="VLE22_27385"
     CDS             15103..16320
                     /locus_tag="VLE22_27385"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB18205.1"
                     /translation="MSESGMSRRTFAAAASLAVLRPERVLGANDRLRVAVIGCGGRGL
                     VGEVLEFARETNVELAVVCDTWRQHREKAAAMTKEALGSEPKMLVHYQDVLALKDIDA
                     AIIATPDHQHCTMLGDAIRAGKDVYIEKPLAMNMKELIEAVDTVKKSDRVVQVGTQVR
                     SFASSVAARAFVAAGGMGRIFKIEQSRNSYRPYWHSYAERPVAESDVDWKAFLMQHKP
                     RPWNADQYAGWYGYRDFSLGPQTGFMSHFVDLVHYVTGAKFPSRVTATGGIYRWKDQR
                     TAPDTFEALLEYPEGFLVRYNTTFGTDNNNFLKFFGTRGVMDATNWDKPWALIGQRGE
                     PDAIAPGTQIPEVESTPHMKNFLECLRTRKQPNAPIDAGYQHAVACLMADEAWVRGTR
                     MVYDPLKRKVQAG"
     gene            16329..17279
                     /locus_tag="VLE22_27390"
     CDS             16329..17279
                     /locus_tag="VLE22_27390"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HSB18206.1"
                     /translation="MTTTRRTFLRNAAGAAALAGAASRAVAQQEPYQFGCETLPYRAQ
                     TFERALEGIRKAGYKYVMPTPTHAGKPVFTTALTAAERASLRRQISDAGLTPFMSFVG
                     LGSGLSNEEGLTKYLQELDLCAEFGIRTVVGIGPWYYTKFPTVPKRARDWERETDAYY
                     ALLEKGVRHAETIGMTITLKPHTGITANAKACLEVVKRIPSERLKISWDAGNVSFYEG
                     IYPDPDLPDLAPHVKSVCIKDHLGGRAEANFPVPGEGQIDHDLMFKILFGAGFNGPIA
                     VERADGKDNAAKMDPAVIDERLAAARANLVPILEKYAKRA"
     gene            complement(17291..17920)
                     /locus_tag="VLE22_27395"
     CDS             complement(17291..17920)
                     /locus_tag="VLE22_27395"
                     /inference="COORDINATES: protein motif:HMM:NF039786.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HSB18207.1"
                     /translation="MRFYDFARAAASLVGLLALLAITPTTAQAHCDGVDGPVVAAARQ
                     ALDAGNVNLVLQWVQPEGEPEVRAAFAHALAVRKLGSGAKELADRYFFETVVRVHRAG
                     EGAPYTGLKPAGRDLGPAIPAADHAIEHGSATQLRELLMGSLDAGLQERFRRVTAAKD
                     FRADDVDAGRRFVKAYVEFVHYVEGVSQAATGPASPHEHNAPEPAHAHN"
     gene            18098..20860
                     /gene="uvrA"
                     /locus_tag="VLE22_27400"
     CDS             18098..20860
                     /gene="uvrA"
                     /locus_tag="VLE22_27400"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSB18208.1"
                     /translation="MLDRISVHGAREHNLKNIDVEIPRNTFTVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVETLSPYARQFLDQMERPEVDSIDGLSPAISIEQKTTSRSPRSTVGTI
                     TEIYDYLRLLYSSIGVPHCPDCGHEISRQSTEQILQQIMSLRMDERVMILAPVVRGRK
                     GEYRKDLEKLARQGFLRARIDGELRSLEEPVKLDRRKNHTIEVVVDRLLVKAGLEKRV
                     EASIQTATKLANGLVLVAVVDGDERIYSQKLACPDCGASVPQLEPRSFSFNSPYGACE
                     SCTGLGSKWTFDPAKVIVDFSKPLLEGGLGPGAGSAYMTHMLSDAARRSGIDLSQPFE
                     QLPKKTQSLLLQGGNGFPGILTALEQTYEEASEGYREWLMDYMSATACPACHGKRLRP
                     GSLAVRVKGLSIAEFTGMPVARALPVVFAWTLTERERIIAGRIVEEIRNRLEFLNSVG
                     LDYLSLDRSAATLSGGEAQRIRLATQIGSKLRGVLYVLDEPSIGLHHRDNSRLLDTLS
                     RLRDLGNTVLVVEHDAETIERADYVVDLGPGAGRLGGDLVAAGTPDQIANDPRSLTGQ
                     YLSGVRSINPPVLRRRGNGKRLVVHGAREHNLKEIDVEFPLGVFTVVTGVSGSGKSTL
                     VNDIVYRALARELYNSREEAGAYGKLSGLEHIDKVIRIDQSPIGRTPRSNPATYTGVF
                     TPIRDLYAMLPESRERGYKPGRFSFNVKGGRCEACQGDGLKRIEMNFLPDVYVTCDIC
                     RARRYNLETLQVKYRNYSIADLLEATVEEASGVLENIPQIRTKLETLRDVGLGYIHLG
                     QPATTLSGGEAQRIKLAKELSRRQTGRTFYILDEPTTGLHFDDVKKLLDVLQRLVDLG
                     NTVVVIEHNLDVVKTADWIIDLGPEGGEEGGQVVVAGTPEQVVRSRKSYTGQALVKVL
                     RNGRNG"
     gene            20857..21816
                     /locus_tag="VLE22_27405"
     CDS             20857..21816
                     /locus_tag="VLE22_27405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18209.1"
                     /translation="MSKYAIHPLDFSGLKTIPIRERGGKVGAEDFAGPHVPGTGFGGW
                     LDSLPKVLAADSLRAVVSALQAARAAAKPVIWGMGGHVIKCGLGPVLIDLMRRGYATG
                     FALNGSASIHDFEIAIAGRTSEDVEAVLPDGSFGSAEETGREMNTAIAEGDRNSIGIG
                     EALGRRIEVLADPAYQSSSLLYEAWARGVPVTVHVAIGTDTPHTHPAVDAAAIGRATH
                     RDFRLFCSMVAELNGGGVYLNVGSAVVLPEVFLKAVSVVRNLGRPLATFNTVNFDFLQ
                     HYRPKVNVVERPHSGAGGQGFAITGHHELMLPLLAAILIELDA"
     gene            21813..22574
                     /locus_tag="VLE22_27410"
     CDS             21813..22574
                     /locus_tag="VLE22_27410"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HSB18210.1"
                     /translation="MNEHESIGQSAIPEAPSRAEENPFWNYQDLALFLTSGIPALLAG
                     ALIVKALFYGFSLSGQGQAPELLSAQFLAYALWYVVLYMILHVKYGRPFWESLGWVRP
                     LERFGRYALLGVLLAFAVAIAGLFLKAPDIEMPMMRLLSDRTSLILVGLAAATLGPIC
                     EELAFRGFILPLLVRSMGVVLGILLTTLPFALLHGPQYAWSWRHLLFIVLAGASFGLL
                     RLRTGSTAAATVMHAAYNLTFFIAFLFQSKNLPVK"
     gene            22571..23599
                     /gene="mtnA"
                     /locus_tag="VLE22_27415"
     CDS             22571..23599
                     /gene="mtnA"
                     /locus_tag="VLE22_27415"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HSB18211.1"
                     /translation="MIETIVWTDAGVVMIDQTRLPKEVTYMTCRDYREVAGAIRSMVI
                     RGAPAIGVAAAMGVALGVLRADPGKLDEEFKTICDTLAGTRPTAVNLFWAIDRMKRVY
                     EGVRSRPVEEVRARLVTEAQQIRIEDIAINEAIGRNGATLVPDGKTVLTHCNAGALAT
                     AGYGTALGVIRAAVAEGKKIDVFADETRPFLQGARLTAWELQQDGIPTTIITDNMAGH
                     FLRSGRVGCVVVGADRIAANGDVANKVGTYPLAVLAKENGVPFYVAAPVSTLDLSLGC
                     GDQIPIEQRPPAEVTHVFGVAVAPEGIRVENPAFDVTPNRYVSAIITERGIAQPPYTE
                     SLGRLMLA"
     gene            23604..24146
                     /locus_tag="VLE22_27420"
     CDS             23604..24146
                     /locus_tag="VLE22_27420"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSB18212.1"
                     /translation="MRKFVLFAACVMLSAALHGAGELSGRRAPGFALPNIADPKLGYH
                     DLADYRGRIVLIEIMQTNCPHCRDFSRVLEEVKAKYGNRVAVLSVVNPPDTQRTVAAY
                     TAATKTTMPILFDCGQMAVSYLKVTPQNPAINVPHLFLIDGEGAIRDDYGYSPANQAI
                     FEGKALFALIDAMLAGGKKK"
BASE COUNT         4705 a         7493 c         7553 g         4478 t
ORIGIN      
        1 cggaacggcg acaaggaatt tccttcgggc agctacgtga ttcccctcgc gcagccggcc
       61 aagcggctgg tacgaaccct gctcgaccca aacatcccga tggaggaaca cttcgtcaag
      121 gagcaggagc ggcgccgcaa aaagaacctt cccgacgaga tctacgacgt cactgcctgg
      181 aacctggcac tgatgttcaa cgtggaatgc atcccggcgt ccgaggcgtc gcagggcagt
      241 ttcaccccag ccggtgaccg aaccccgcca ggggcggtag ccggtgataa cgcgactgtc
      301 gcctatctgg caccatgggg cacgctggcg tccggccgcc tgctcgcggc ctcgctccgg
      361 tccggcctcc gtgtctggag tgcggacaag cccttcacgc tgggcgggcg gaagtatccg
      421 tccgggacct tgattttcaa agtgaaggag aacgccgctt cgcttgcgga ggcgctcgcg
      481 aaactggctg cctccaccgg agccgaaatc gtcggtatca acagcagttg ggtggacggc
      541 ggtatcagtc tcggcagcaa tcacgtggtg ccgatgcgga agcccgccat cgctctcgcc
      601 tgggacaccc cggcttcgcc tctctccgcc ggatggaccc gctacgtgct ggagcgccag
      661 tacgactatc ctgtgacgcc ggtccgcacc cggcagctcg cctcctcggg gctgcaccgc
      721 ttcgacgtgg tcatcctgcc cgagccttcc ggcagctaca tggacacgct cggagccgcc
      781 ggcgtcaagc ggctcaaaga ttgggtgacg gacgggggta cgctcgtcgc cctcggcggc
      841 tcggttgcgt ttctggcgga ctcgaaggtg ggtctgctgt caacctctcg cgaaaacctg
      901 gcgaagcccg tcgagcccgg aaagccaccg gagcagaagg agaccgcccc ggccaaaccg
      961 gacgcgcaag ccccggcaag ggtgcctggg aaactgctca cgtcggaaac ggagtacgaa
     1021 aaagcgcttc aacccgaggc ggaaccgccg gattcggtcg ccggagtcat tctaaaggca
     1081 cgggtggatt ccgatcactg gctcggcgcc ggcctttccg ggaccgtcaa cgcggtggtc
     1141 gtgggacggg agatcttcac cccgatcaag ctcgacaaag gcgtgaacgt cgcgacgttc
     1201 ctcggtccgg accagttgct ccagagtggt tacctgtggg aagaaaaccg taagcagttc
     1261 gcttacaagc cgctggcggt ggcccagccg gacggccgcg gcgtggtcat cggtttcacc
     1321 gccgacccga acttccgcgc ctacgtggac ggcctcaacg tcctgttctt gaatgccgtg
     1381 ttccgaggac cggcccacgc acgtccggcg ccctgatcag aatccttcga gcagatggcc
     1441 gagcttttct ttcttcgtgc gaaggtagat ctcggtggta tcgctgggtg cgatgatgca
     1501 gggaatccgc tcggagacct cgacacccgc gcgttcgagc gcctggatct tgtcgggatt
     1561 gttcgacagc aggcggacgc tcgtcagccc gaagtgccgc aggatttccg ccggcagctc
     1621 atagttcctg aggtcggcct cgaacccgag ttgctcgttg gcctcgacgg tgtcggcgcc
     1681 gtggtcctgt aattcgtagg cgcgcagctt gttgagcagc ccgatgccgc gcccttcctt
     1741 gtgttcgtag acaagcaacc cacggccttc gccggcgatc cgggccaggg acagctcgag
     1801 ctgagcgcgg cagtcgcacc gcaggctgtg aaacacgtct ccggtcaggc attgcgagtg
     1861 gatgcggacg agcggcggcg gatcgccgga gacttcgccc atgcgcagaa cgaccgcctc
     1921 ttcgagacgg ccgccgtatt cgcccttaaa accgaatatc cggaacagtc cgaagctggt
     1981 ggggaggtcc gcctcggcta ctttacgtaa ccgggctgct accgctgggt tttccatcag
     2041 acagaaacga aggaacctgg aggttccgcg ttctcttaat tataatgaac ggcaacgcta
     2101 catgctggac cagcacctga tcagactcct ggtgaagctc gggatcgtgg cttccctggc
     2161 gagcattttg gtccggtcgg acgctttcaa gcgcatgctc ctccgggaac aacgcaccct
     2221 gaaccagcgg ctgctgcttg ctttatcctt ctcgagcatc tttgccacag gcgtcgccac
     2281 ccgagtcgtc acgcggtcct atcaggcggt ggacctcggg ctggagggga gcctgctatc
     2341 gggcatcctc ggcggctatg taaccgggct tgtgtcggga atactgattt cagtgcccgc
     2401 catgttcaac ggcgagtacc tcagcatgcc tctcctcgcg ggggtcggcg tactcggcgg
     2461 gctgctgcgc gattgcgcgc cggacaccga agacatctgg cacttctcgc cgtttttcga
     2521 cctcagcatt taccggctct tccggcgggg ccacgatcac cgccgctcgg cgttccacct
     2581 gttcttcttg ctggcgatcc tcttcgccga gttcctaagg atcacgctcg gccacatctt
     2641 caaggactca gtcttctatc tctatcccga gtgggtctcg ccgcacccgt tgacgattgt
     2701 cattgtctac atcacgacgc tgttcgcagt aacgctgccc ctcaagatct ggaacaacac
     2761 ccggaacgaa agcaagctgg ccgagcagca gcaggtgctg ttgcgggcgc ggctcgaggc
     2821 gctctccagc cagatcaacc cgcattttct gttcaacacg ctgaattctg tctcctcgct
     2881 gatccgtacg gacccgggcg aggcgcgaaa cgtcgtctac aagctctcca acatcctgcg
     2941 ccgcctgttg cggaagcacg acaacttcaa cccgctgcgc gacgaactca atttcatcaa
     3001 cgattacctc gcgatcgaga tggtgcggtt cggagacaag ctccacttcg agaaggacat
     3061 cgctccgaac accctggacc ggccggttcc gagcatgctg ctccagccgc tggtggagaa
     3121 ctgcatccgg catggcctga gcggaaaagt ggagggcggc acgatccgag tcagcagcca
     3181 actggcggac ggccggttgc acctctctgt ggaagatgac ggcgtgggca ttcccgaagt
     3241 aaaattggct accctgcttg aacagggcat cggcgtcagc aatgtcaacg agcgcctcaa
     3301 ggttttgttc ggaaacgact accggatgtt catcgacagc aagcccggta ccggcacacg
     3361 catcgagatc cagattccgg aactccagct ccccggaacg ggtgtctcct gacttcttac
     3421 tgcggagcac tgattgacaa acctccaaac ccctgtactc atacccgaga cgaacggcgc
     3481 gcaccggacg atcgccggcc ggacccgcag ggtctacgac cgcctggcgg ccgtctatcc
     3541 ggtgtcaacg ttgttcttcc attcgacggc gcacaagcgc gcgctggcgg tagccggcct
     3601 tgtcgacggc atgcgcgtac tcgaagcggc taccggttcc ggcgaaatgt tccggcggct
     3661 cgtgcgcgcc aaccgcggcg gtgaaacggt cggcttcgac ctctctccca acatggcggc
     3721 tcggacgcac cgcagggtcc gccgggagtt ccccgctgca acaaccctct gccaggcggt
     3781 ggatgcccgc tacctaccgt tctgcggtga gagcttcgat ctcgtcgtct gctgctacct
     3841 actggagttg ctctcatcgg aggacatcct gctcgcgctc agcgaaatcc accgcgtctt
     3901 gcgccctggt gggagattgg cgctggtcgc gattggacag aacacggagt tcttcaaccg
     3961 cgtctaccgc gtactcggca agatcgcgcc cgcgttttgg ggacgccaga tcgatgggcg
     4021 gctgattgaa ctcattgaga ccgctgactt ccgtctcctc agtgaggaac acgttcgcca
     4081 gagcctgtac ccgtcgcgtg tgcttctcgc cagaaagtag gccgcagccg gcatctgatg
     4141 taaagtattt tcggaggttc tcctcatgca atccgaccgc aggcagtttt taaagaattc
     4201 cctgggcgtg tcggcggccg tctccgccgc cccgatgatc gtcccgcgcc gtgcttgggg
     4261 cgcaaacgac aagcccgcct atgccctgat cgccgccggc ggccggggcc gttacctgaa
     4321 caaggttttc aagaagctcg gcgcacagtg tgtggccgtc tgcgatgtct atgagccgta
     4381 cgtcgagatg gcgctcgaag attctcccga cgcgcgtgcc tacgtcgact acaaagagct
     4441 gcttgaaaag gagaagagtg ctgacttcgt catcctcgcc ggacccgacc atcatcattg
     4501 cccgatgctg ctcgcttcgc tagccgccgg taaggacgtg tacgccgaga agccgctctc
     4561 gcgcacgctc gaagagagcc agaagatgat ccaggcggtc aagcagagcc agaaggttgt
     4621 tcagatcggc atgcagcggc gcagcgcccc ggtgatccgt tcggccaaac gcctgattga
     4681 cgacggcgtc ctcggcaagc tcgccatggt caagccaatg tggaactgga acgtctccgg
     4741 cccaatcgac aactcccccc ttcccggtaa gctcgactgg caaaggttcc tcggagacgc
     4801 tccccagcgc gagctggagc cgatgcgctt tcgccgctgg cggtacttct gggattacgc
     4861 gggcggcaac atgaccgacc agggaacgca cctgatggac gtcgtcctgt ggtttaccaa
     4921 ctccggaccg ccggtctcgg ccatctgcaa cgggcaggtc aacaagatga agggggcgga
     4981 acaccccgag gtcttttgcg ccgtcttcga gttcccgagt ttcctcgtca cctggacgct
     5041 gaactactgc aactcgtacg agaacggctg gtccatcacg ttcatgggcg acgaggggac
     5101 gatgattctc gacgaggacg gctaccgtgt ctatcaggag ccgtgggcct tgccgaagaa
     5161 ccgcgagccc atcttctatg caaaggtggc ggtgcccatc gaagcgcacg tccagaactt
     5221 cctggactgc atcaaaaccc gggagcagcc cacctgcgac gtggaatcgg ccgcgaaggc
     5281 cgtcgccgga ccacacctag ccaacatcgc gttccagcaa ggccggaagg cgacgctggc
     5341 cccggatatg gtaacggtct cttagactct atcggacgac accctcgagc gggctgctgg
     5401 cgctggcgta cagtttcttc gggatccggc cggcgagcca ggccaggcgg cccgcttcca
     5461 ccgcgagttt catcgcaacg gccattttct cggcgtcctg cgctccggcg atggcggtat
     5521 tcatgagcac gccgtcggcg cccagctcca tggccacggc cgcgtccgac gcggttccaa
     5581 ccccggcgtc cacgatcatc ggcactgctg tgatcatctc ccgaagaatg cgcaagttcg
     5641 cggggttctg aattcccatg ccggacccga tcggcgcggc cagcggcatc accgccgcgg
     5701 cgccggcgtc gatcagcttc ttcgcgtaga tcaggtcgtc gttggtgtac ggcatcacga
     5761 cgaacccctc cttgactaga atccgcgtgg cttcgagcag cccctcgttg tccggaaaca
     5821 gcgtcttctc gtcgccgatg acctctagct tgatccaatt cgacagcccg acctcgcggg
     5881 cgaggcgcgc cgtgcggacc gcttcgtcgg ccgagtagca gccggccgtg ttcggcagga
     5941 tgaaaatctt ggaacggtcg atataatcca gcaaagattc cttcgacttg tccgtgagat
     6001 tcacccggcg cacggcgacg gtgaccatct cggcgcccga agcggcatgg cagcgggcca
     6061 tttcctgaaa gctgcggtat ttgcccgtgc cgacgatcag gcgcgagcgg aactcccgcc
     6121 cggcgatcac tagattgtct ggcatacttt cattctagaa ccagggagtg gagaaatcat
     6181 gaaacggacc ttgctgcttt tgctcttcgt gctgccgctc gccgcgggtg attttcgcct
     6241 cggccaagcc gcggtgaaga tcaccccgcc cgcaggcgct ccgatggccg gctactacca
     6301 caaccgtgcc gcctcaggcg tccatgacga ccttttcgcg aaaacgctgg tgctcgaaaa
     6361 ggatggcgag aaaacggctc tcgtcgcctg tgacatcatc ggcatgccgc gcggtgtggc
     6421 ggaggaagcg cggcggctgg ctgagaaaga gaccggcatc ccgggcgccc ggatcatgat
     6481 ctccggaacc cacgcccaca ccggccccgt cctctacgaa gagggttcgc gatacaacct
     6541 tgagggcgac atgttgcgaa tcgccaggga atacatcgcc gcgcttccgg cgaagatcgc
     6601 agagagcatc aaactagcgg attcgcggct tcaaccggca aaagccgccg ccggcgttgg
     6661 gcacgaggag tcgatcagct tcaaccggcg tttcttcatg aaggacggca cggtcggctg
     6721 gaaccccggg aaattgaacc cgaacatcgt gaggccggtg ggacccatcg acccggatgt
     6781 cggtgttgtc cgcttccagg ccgaggatgg tgactcgctg gctacctacg tgaacttcgc
     6841 tctccatctg gatactgtgg gcggcgtgga gatttcggcg gactatccgt atacgctctc
     6901 aaaactcctc ggccgactga acggcgcgga catgctgacc gtgttcacga tcggcgccgc
     6961 cggaaatctc aaccacatca acgtaagaac cgccgcgcgg cagaacggac atggggaagc
     7021 ggaacgcatc ggcggcgtgc tcgccggcga agtattgaag acctacaccc ggctggagga
     7081 tatcgcggca acctccgtgc gcagccggag cgagatcgtg cgccttcccc tggcggagca
     7141 tgagccctcc gacgtcgaat gggcacgcgg cgtggcgccg aagttcggca agcccggcgc
     7201 cgccccgttt ctagacctcg tgaaggcgtt ccgcgtgctc gacgtcgagg ctcggaaggg
     7261 caagccgatc gacgccgagg tgcaggtcat cgctctcggg gaccaagtcg cctgggtcgg
     7321 gttgcctggc gagatcttca ccgagctcgg gatggcgatc aagcgtgcct cgccgttccc
     7381 gtacacgatg gtggctgaac ttgcgaacgg ctcaatcggc tacgtgccga accaagagtc
     7441 atactcgcaa ggcgcttacg aggtagtcag cgccagatgc gcagccggtt ccggcgagat
     7501 gctggtggac accgcgctcc gcctgctgaa acagctcaaa gccaccggcg ccgctacgaa
     7561 ctgaatctta ctgtagcgag cccgttccga gcttcgcatt cggcggaggc gtatcggttc
     7621 gctgccggcg aagtgcgatc agccgggcgt cctcggcagt ttgcgtggct ccacgcgccg
     7681 cctccacgat gacggatcgt ccggccttct gcgagaagag ccgttccgcc tcggcaagtt
     7741 gcacttgcgc tttccggagc gcccccggag cgtgctcctc cgcgccctgc gccgcggcaa
     7801 gttgcaccgc gttacgcgcc tggtagacct cgagcagagc ctcataccgg tcgagcgaca
     7861 gctttggtcc gtccgcggcc gccacacgcg agcgggcgtc ctgaagatcg agcgtgtatt
     7921 caccccgttt cagcagttcg tactttgctt ccacctgctg cacgttgccc cgcgtccccg
     7981 gcaggactgt attttcaagt accacgacgt cacttgggtg agttgcggcg aagtacggtt
     8041 ccgcggtgag gatcagcgca aatgactgta gctcggtgga agtgcgcagt ttcccgcgat
     8101 tgtcgtgatc ggtaatgact tcccccagat tggtggagcg tccttccgga gtgatggccc
     8161 acaacacgta cgtcaggtag ttcggcccaa acctgtgggg tggttccagc ccccggaact
     8221 ccgcctcgat cccgaaagct cctttgtggc ccttgacgcg cgcttgtcct tcggcgcgcg
     8281 gcaataacac ggtgccctgg aaaccgatct tcgtcgagtc gccgtcagga cggtaactga
     8341 tcgccttcgt ggtgcgggat agaacgttca ctcggtagat gggcgccgat ccaggagccg
     8401 gtggctgagc cggattcaca ttcacttgtt gcgtgggatt gggctcctgg gcgatcagaa
     8461 gaatcggcag aacaattact ccaagcggtt tcatcgttca agcctcctag aaaaaccttc
     8521 actcctctgg aaaggcgggc ggagagtgcc ggagcagcct ccgcaccgcc ggtcctgcct
     8581 gtccgccgta tcgagactac gccgtcaggt cgcgggggtg acggactcca cctcgatggt
     8641 gtcgtccttc gcactgccct gcatcgtcac tttctggccg acaaatttct tcgctttctc
     8701 cgcgtcgctg aggtggtaga tcttcttgtc ggcttcgttc acgaacacga ccgtgccgcc
     8761 ggagttgatg cagtttttgt gtttctctcc ggtgaacgtt ccggtctgag cgcatttttc
     8821 gtcggtaacc cagccgaccc actggtccgc cagggcaagt ccggcggtga gggccagcag
     8881 aagcgcgagt gtagagagta ctctcatcgc tgtttgtaac ctcctctggt atgaagctga
     8941 cgccggcccg gacagagaac ccagaatctg cggccgccgc gcttcacttt atggttctag
     9001 ataaacttga gcaacattga ggccaatccc gaaagccgct ttagtccgaa ctggttgcgg
     9061 ccggccgtcg acagaccgcg gcctgcgggg gtggtaaaag tttcccggca cagtagcaga
     9121 aacgtcagtg tcagcccgcc cgtccacacc ggaggtcgaa cctggcagca gccggggctc
     9181 caagagagcc gagatgggct agactgggtg tggtagcatc ccattcgccg gcgaatgcgg
     9241 gcccttagct cagtcggtta gagcagcgga ctcataatcc gttggtcgta ggttcaagtc
     9301 ctacagggcc caccagccaa tccaagagtg cgcccacccg accacctttt gatcgtttcc
     9361 agataccgtt ccattcgggt agccgcgtca actagatcgc ggcgtgcgac gatgtcgtag
     9421 cgggggcagg agtcggaacc gtagtcagcg cctatcgggc gctcaatggc ccgccacagg
     9481 ctttccaaaa cccttctact tcagttcttc cgtcatcttc tttatttccg gttgcagatc
     9541 gcccatcaac tgcatcatca cgggcatcga gcgttgcatc acctgcggca ttttctgaag
     9601 caaggctttc ccggctggcg atttgtaaaa tgccaaaatg ccgtcgatct cctcttccgt
     9661 gtaagtgtcg gtgtagatct tcacgagctc aggcttggct ttactgagac ggtcggcgat
     9721 cagcgccatg atcttcccct gcatttcgct ggctttcgca cgcttctcag caggcatctc
     9781 cgtcttgttc atctccgccg tcagtacgcc cttcatcggt tcgagaaatg acttcatcat
     9841 ctggccggtc tgcgtcaggt tcaacagttc ttcggccttt gcaaacttgc tggcctcgtc
     9901 ggcccgcatg ggagaggcac accacaaaag ggtaacgccc gtaatgagag caatggctat
     9961 gtacttcatg acgtgattat acgcttcatc cctgatacat aatcgaccgg aactcctgac
    10021 ttttgaggcc cttaggggtt gacggagttg accgacacga cggagccatt cctgagtacc
    10081 aagacagtct cttttttcgg aggcccgcga cgcagataga cgagggtttc catttcttgc
    10141 ccctgctcca ctgaagtggt gaccatattg ggttttccaa accgcgccaa taattccgac
    10201 ttctccatac cgaccggaac gctctcggct gtgggaaaag cgggctgctt gggcttctcg
    10261 gcacgctgcg gctcttcctt ccgcatcggt cgggtggcgg gcgctgctac ctctggcggc
    10321 ttggcggtgc gcggcggctg gaccgtctcc ctcacgtcac ttaagcccgg aggcgtgctc
    10381 tgatgccacc accacaccaa cacacacagc acggcgatca gcagcagtgc cacgagcagg
    10441 gcgttggccg cgttcctcgc ctttgctgat tcgccttcag ggactgtgtt cactgatgcc
    10501 attctaagcc cgaaaccacg tcggtcgtgc ggcaaacaca aagcacagcg ttagccgcca
    10561 tagacggcac tccgagggat tcacaatccg tcggccgctg gttcgagtcc agcggggcct
    10621 actaatcaaa tcaagagctt gcaatcggtt ctacgccatt actgggggca ggactcccgt
    10681 gcctctcttc tcatgcgctg cacaagcagg atcagctcac ggtcccgcgc gttgctgcag
    10741 ggcgcatcgc acgcctccag gtctcccaag cggaggcagt gcccccggca ttgctccgag
    10801 cccaacagcc tgacacgcga ggcgtccggt tccggttcgg ggagcaggtg gcgcagtctc
    10861 ctggagagta tcgagacgga gctgccgcaa ccgtgctcga agaccagcag gttgacgtcc
    10921 ggatgggtga tcacggcttg taggcccagc aggcgcaggt ccggatccag gacgaaacgg
    10981 tcccacgttg accattcgtg tctgcaagac ctgcagatct ggaagggcct ggccgcgcgc
    11041 tcgacggtca cagcgggaat tataagccaa atgtgaggag tgcgaatact gtgcgcgcgg
    11101 agcacgacaa gcgcctgccg gccttcccgc tgatcgcctg cagaaaatcg gtattcggga
    11161 gcccggccga agacgcgagt agaatgcgtg cagttctacc ggtgaggaga tctcgcgcat
    11221 atgcgcgatt cataccagga ggagatcagc cgttcgtccc gtagagcgcc ccggtggtcc
    11281 ggtggagcat ccggacaaat gccagagtaa caatccccag catccggtcg aaacagcatt
    11341 cgcactcgag cagatgtttc aggaccgagg gcacgtcggc atcgggcaga cggttgattc
    11401 cgtacaggca gagagcgtct tccgtgaggt gcccgaaccg gaagggcgat gggaaaaaaa
    11461 cgcgggagcg tccgaggagc gccacggacg ctcccgccga gtccttcgag gctgccttgc
    11521 cgttgaccat cgcaactagc ggaattgtac gtccaatgca ggacgcttgc aagattcgaa
    11581 aaggtaagcg ggaccgtact caccttggtt agcgccggag ggcgccatca ggacgaacgg
    11641 ccggggtcgt tcaaacggcg gtggtagcgc cagttcgggt cgatagagtc cgcatcgaag
    11701 ccgtacggcg ccactccgtc ggtggcgaaa cggtacagcg ccacagtaaa gattcctttc
    11761 accgccgagg agacggccgc gaggagcagt atgtagacca tggttaggat gatggcggcg
    11821 ccgagatcat acggataaac gagccacccg agcagcagcc cggggaccaa cagcagcaag
    11881 ttcagcaggc cgaaaccgaa actgcccgcc acctgctcgc cccagtgttt ccggaatagc
    11941 tccgacgagc ggagcatgcc gtcggagacg ttccggtttt cgatgataat caccgggatg
    12001 atcagatagg tgatcagcgc ccatccgacc gctagacctg agctgagaag ccggacaagc
    12061 ctgttgcccc ggtcccgcaa ccagctcaga acgattccaa cggttgccgc cacaaatgcc
    12121 caggctgcga tgcgcgtgat gcggctcatc gccacccgca ggccctcggc aacggtgggg
    12181 ttgccgcccg aaagccggat gttggcgcat gcgaccagag cgctgttgaa gaagatgacc
    12241 acgaaatagt tcaggtaata ccagacaaac agaaccgcgt agtcggccca gccgccagcg
    12301 ccgtccttga tggcgagcag cgtgccggcg aagtaaagcg ggaagaaaaa cgcggccgcc
    12361 accaggacag tagatatcgc gctcatcacc ggaaacagca ggatctccgt atccgcgccg
    12421 agcacggcga aactttgttt atacagcttc caggtacgcc ccaatgtccc cacggctgtt
    12481 ttacccccga gccatgctat caccaccaga agacgataaa agcctggtag aggttcggcc
    12541 aaaagcgagt gagccgagga tgagacccgc aggcgccaga gtgaagctaa gcaagccggc
    12601 gtaggtggga tctatagacg gtatggcgag gcaagtgatc ggcgggatgg cgctccctag
    12661 aaaagcacaa taggcgcccc agacattcgc tcgtttccaa tagatgccgc cgatcaacac
    12721 ggtcagggtg gcggcaaaag acagagaccc gcttagtgtg atgtaccgga ataccgactc
    12781 cgggaaatgg tagaacgatc cccaaatgta aatgaaagca ccgatgacaa gcacacccgc
    12841 ctgtactgcc ttcactcgcc ccttgccccc gggaagccgc tttgtgtgcg gcccgatcac
    12901 atcttgaaac aggatcgagc tgaacgctag aaaatagctc gagtaggtag acatcgaggc
    12961 tgagagaaac ccgatcgtaa cgatgccgat cagtcccgcg gggacgatgc gcgccagcat
    13021 ctgaggcagg gccatgctcg actcgcccgt ccgcccaagg atggcgagtg caccgatgcc
    13081 ccagaccatc gggaacgccg cccgcatgaa cataaagagg ctgctgatcc ggtacagctt
    13141 cagcgcggtg gctatatcgc gcatgctcag gatgcgtgcc accaccggct gccacgaact
    13201 ccagccactc aggtagtaga gcagagtcca cgagaggaac agcgggccgt agcggggcgc
    13261 cttccagata tagaagccgg catcgccgta ccgccgatct accgcctccg ccatacctcc
    13321 ccatccaccg gcgttcgaga agattagcaa ggtagtgatg ccgactccgg caacgatcgc
    13381 gatgaactgc accacgtcgg tgagtacaac cgaatacatc ccgccgagca tggtgtatgc
    13441 caccaccaca acgagcatca cggccatcac gagcaggatt ctcgactccg ggatgcccat
    13501 gataatcgcc atgaagttgc tctcggcttg gagaaaaata cccatgttga gcacgccgac
    13561 gatgaaggtg gccaggcccg cgatggtgcg gacgcgcgtg ttgaagcggc tctccatgta
    13621 ctccggcacg gttcgtatct cgagttcgcg gagctttctg acaatcaggc cggtccaacc
    13681 gacgaagaac attgttacgg cggcgaggag agcgacgata agggccacaa atccgctcaa
    13741 atacgcctct tcagcgaaat aaatgagcgt aatcagcccg gtttctgtcg cggcgagcgt
    13801 ggccacaccc cagaccggac cgagacgccg ccccatcacc aggaattcgt ccacggatga
    13861 ggagtgcgcc cgcgccctga tacccaggta cgcgtagaac gcgaggaacc ctagaaccaa
    13921 cacccaatcg aagaaagaga aatgagagtt cactggcgaa gctcgaccgg ttgtcccgtt
    13981 cccgccgatt tcaccatcgc tgcgacgacg cgcgcggctg cgagaccgtc ctcgcctgtc
    14041 accatcggct cgcgcccgct ggtcagacag tctatgaaat gctcgatggc ggccacgccg
    14101 aatccaaccg gcttgccgtg cagatcagcg gcgcaggcga cgtccggata agcaacgccg
    14161 cctgtggtgt acgtcttcag catggcgtga tggctggcgt tgacgtatgc gctcgccgca
    14221 ctgccgatga actcgcactg gaagtcgaac acgctgggcg acgattcatt cacgacccag
    14281 cagttttcca ccacggccgt ggccccgctc gatagttcga ggatggattg gaaaaaatct
    14341 ggagtatcga tgccggcgtt cttcagcacc agcgagcggc tgacggaata gactcgcgta
    14401 acttctgcgc cgctcagcca gcggatgata tccaccacat gactgcctag aaagtgagcc
    14461 ggggaggaac aagaggccca gccgagcatc ttcgtcggca caaagagcgt gtcattcagg
    14521 cggatgttga tcgcaagcag gtcacccatt tctccggatt ggagcatccg gcggatgttc
    14581 acgaacggca ggttccagcg attgtggaag tccaccatca gcttgacgcc tttggcgcgg
    14641 cgggcatcaa ggatccgctc gcactcctcg atggtggttg ccaaaggttt ttcgacgagg
    14701 atgtcctttc cggcatcgat ggccgcttgt acgatttctc catgcgcaaa gtccggcgtg
    14761 gcgatcgcga ccgcattgat ctcggcatcg gcgagcagtt cgcgccagtc cgccgaatgc
    14821 ttctctccgc gccatagtta cgcgcggcct ttgcagccct gtcagcatcc tgatcactga
    14881 tggcaatcag ccgcacgaac ggggaggctg cgtacgtcct cgcatgaacc gacccccagg
    14941 tcccgcagcc gatcaacccg atccctcgtt tcttcattgt gttctcctgt aggaaagccg
    15001 tagaggcacg gtgtctcgaa taaccgggtc atcatacccc cgttccctcc accggcgcac
    15061 cgtccaggcg ggtatgatag ctgcgatagg aaggagggca atgtgtctga atcgggtatg
    15121 agccggcgga ccttcgccgc ggccgcttcg ctggcggtgc tgcggccgga gagggttctt
    15181 ggtgcaaacg accggctgcg agtggccgtg atcggttgtg gcggacgcgg actggtcggt
    15241 gaggtcctgg agttcgcacg tgaaaccaat gtcgagctgg cggtggtctg cgacacctgg
    15301 cgccagcatc gcgaaaaggc cgctgccatg acaaaagagg cgctcggcag cgaaccgaaa
    15361 atgctcgtgc attatcagga cgtgctggcg ctgaaagaca tcgacgcggc gattattgcc
    15421 acgccggacc accagcactg caccatgctc ggtgacgcca tccgcgccgg gaaggacgtc
    15481 tacatcgaaa agccgctggc gatgaacatg aaggaactca tcgaagcggt ggacacggtg
    15541 aagaagtccg accgcgtggt gcaggtgggc acgcaggtgc ggagcttcgc cagttccgtc
    15601 gccgcgcgag cgttcgtcgc cgcgggtggc atgggccgga tcttcaagat cgagcagtcg
    15661 cggaactcct accgcccgta ctggcacagc tacgcagagc ggccggtcgc cgaatccgat
    15721 gtcgactgga aagcgttcct catgcaacat aagccccgcc cctggaacgc agatcagtac
    15781 gccggatggt acggataccg ggatttctca ctgggcccgc aaactggatt catgagtcac
    15841 ttcgtggacc tggttcacta cgtcaccggc gcgaaattcc catcccgcgt gacggcaacg
    15901 ggcggaatct accgctggaa ggatcagcgg accgcgcctg acacgttcga ggcacttctc
    15961 gagtacccgg agggattcct cgtccggtac aacacgacct tcggtacgga caacaacaat
    16021 ttcctgaagt tcttcgggac gcgcggggtg atggatgcga cgaattggga caaaccttgg
    16081 gcgctcatcg gccagcgcgg agagccggac gccatcgcac cgggcactca gatccctgaa
    16141 gtcgagtcca caccgcacat gaagaacttc ctggagtgcc tgcggacacg gaagcagcca
    16201 aatgccccga tcgacgccgg ctatcaacac gctgtcgcct gtctgatggc tgacgaggcc
    16261 tgggtgcggg gcacgcgcat ggtttacgat ccgctgaaac gaaaggttca ggcaggttga
    16321 gattccatat gacaacgaca cgacgaacgt ttctcagaaa tgcggcaggc gccgcggcgc
    16381 tggcgggcgc cgccagccgg gccgttgcgc agcaggaacc gtatcagttt ggctgcgaga
    16441 cgctgccgta ccgggctcag actttcgagc gggcgctcga gggaatccgc aaagctggtt
    16501 acaagtacgt catgccgacg ccgacccacg cgggcaaacc cgtgttcaca accgccctta
    16561 ccgccgcgga acgggccagt ctccgccggc agatctcgga tgcggggcta acgccgttca
    16621 tgagtttcgt aggccttggc agcggcctca gcaacgagga gggcctcacg aagtacctcc
    16681 aggaactcga cctgtgcgcc gaattcggca tccggacggt agtcggtatc gggccgtggt
    16741 actacacgaa gtttccgact gttccgaagc gtgcgcgtga ctgggagcgg gagacagacg
    16801 cctactacgc attgctggag aaaggcgtgc gccatgccga aacgatcgga atgacgatca
    16861 cgctgaaacc gcacacgggc atcaccgcca atgcgaaggc gtgtcttgaa gtggtcaagc
    16921 gcatcccgtc cgaacggctg aagatcagtt gggatgccgg gaacgtctcg ttctatgaag
    16981 gcatctatcc ggacccggac ctgccggacc tggcgccgca cgtcaagtcg gtctgcatca
    17041 aggaccatct tggcggccgc gcggaagcga acttccccgt tcccggagag gggcagatcg
    17101 atcacgacct gatgttcaag atcctcttcg gtgccggctt caacgggccg attgccgtcg
    17161 agcgtgcgga cggcaaggac aacgcggcga agatggaccc tgcggtgatc gacgagcggc
    17221 tcgccgcggc acgggccaat ctggtcccga ttctggagaa gtacgccaaa agagcgtagc
    17281 cagggtccgc ttagttgtga gcgtgagcgg gttccggggc gttgtgctcg tgaggggaag
    17341 ccggacccgt ggccgcctga gaaaccccct ccacgtagtg gacaaactcg acgtacgcct
    17401 tgacgaaccg gcggccggcg tcgacatcgt cggcgcggaa atccttcgcc gccgtcacgc
    17461 gccggaaccg ctcctgtagg ccggcatcca gcgatcccat cagcaactcc cgcaactggg
    17521 tggcggagcc gtgctcgatc gcgtggtctg ccgctgggat cgcgggtccg agatcgcgcc
    17581 cggctggctt caaacctgtg tagggcgccc cttctccggc gcggtggacg cggacgaccg
    17641 tttcgaagaa gtagcggtcc gcgagttcct tcgcaccgga gccgagtttt cttacggcga
    17701 gggcgtgggc gaaggcggcg cggacttcgg gttcgccttc cggctggacc cactgcaaca
    17761 cgagattgac gttcccggcg tcgagcgcct gtcgtgcggc ggcaaccacc ggaccgtcga
    17821 caccgtcgca gtgcgcctga gcggtcgtcg gagtgatggc caggagagcc agcaggccaa
    17881 cgagtgaagc agcagcccgg gcgaaatcat agaaacgcat gtctttcctc cttggtgtcc
    17941 ggttccagaa taagcctgga aacgccggcg cgcagtgacc gcggtcactc aatcctgttt
    18001 gagcgcgcgc acagttaccg agcggcgccc cctcgttgcc gccccgtaac ggttcgaagg
    18061 acaataagag attggcctgg gaacggcgaa gctcaccatg ctggaccgca ttagtgttca
    18121 cggggcgcgc gaacacaatc tgaagaacat cgacgttgag attccgcgaa acacgtttac
    18181 cgtagtgacg gggctgagcg gatcgggcaa gtcgtcgctg gccttcgaca cgatctatgc
    18241 cgaaggacag cggcgctacg tcgagacgct gtccccctac gcccgccagt tcctcgacca
    18301 gatggagagg ccggaggtgg attcgatcga cggcctcagt ccggcgatct ccatcgagca
    18361 gaagacgaca agccggagcc cgcgctccac ggtgggcacg attaccgaaa tctacgacta
    18421 cctccggctg ctttattcct ccatcggcgt gccgcactgc ccggactgcg gccatgaaat
    18481 ctcgcgccag tcgaccgagc agatcctcca gcagatcatg tcgctgcgga tggatgagcg
    18541 tgtgatgatc ttggctccgg tcgtccgggg gcggaaaggc gagtaccgta aggacctcga
    18601 gaaactggcg cgccagggat ttctgcgggc gcggatcgac ggcgaactgc ggtctcttga
    18661 agagcccgtc aagctggacc gccggaagaa tcacaccatc gaagtcgttg tggaccggct
    18721 cttggtcaag gccggcctgg agaagcgcgt cgaagcatcc atccagacgg cgaccaagct
    18781 ggcgaacggc ctcgtcctgg tggccgtcgt ggacggtgac gagcgcatct actcccagaa
    18841 gctggcctgc ccggattgcg gcgcgagcgt tccacaactc gagccgcgtt cgttttcgtt
    18901 caacagcccg tatggggcat gcgaaagctg caccggcctg ggtagcaagt ggacattcga
    18961 tcccgcgaag gtaatcgttg acttctcgaa gccgctgctc gagggcgggc tgggacccgg
    19021 agcgggttcc gcctacatga cgcacatgct ctcggacgcg gcgcgtcggt ccggcatcga
    19081 tctctcccaa ccgttcgagc aactgcccaa gaagacccag tcgcttttgc ttcagggcgg
    19141 caacgggttc cccggcatcc tgacggcgct cgaacagaca tacgaagagg cgtccgaagg
    19201 ttaccgcgag tggctgatgg actacatgtc cgccacggcg tgccctgcct gccacggtaa
    19261 gcgattgcgc cccggcagtc tcgccgtccg ggtgaagggc ctctctatcg cagagttcac
    19321 cggtatgccg gtggcgcggg cgttgccggt cgtgttcgct tggacactga ctgagcgcga
    19381 gcggatcatc gccggccgca tcgtcgaaga gatccgcaac cggctggaat tcctgaactc
    19441 cgtcggtctc gactacctga gcttggatcg ctccgcggcg acgctctcgg ggggcgaagc
    19501 gcagcgcatc cgtctggcga cacagatcgg atcgaagctg cgcggagtgc tttacgttct
    19561 cgacgagccc tccatcggac ttcatcaccg cgacaacagc cggctgctcg acaccctgag
    19621 ccggctgcgc gacctcggca acactgtgct ggtggtggag cacgatgcgg agaccatcga
    19681 gcgtgcggac tacgtggtcg atctcggacc cggcgcgggc cgtttgggcg gcgatctggt
    19741 ggcggcggga actccggacc agatcgcaaa cgatccccga tcgctgacgg gacagtatct
    19801 gtcgggtgtt cggagtatca acccaccagt gttgcggcgc cgcggcaatg gcaagcggct
    19861 cgtggtccac ggcgcccggg agcacaacct gaaggagatc gacgtcgaat tcccactggg
    19921 cgttttcacg gtggttaccg gagtctcggg gagcggcaag tcgacgctgg tgaacgacat
    19981 cgtctatcgg gcgctggccc gggagttgta caattcgcgc gaagaggcgg gggcgtacgg
    20041 caaactaagc ggcctcgagc acatagacaa ggtgatccgg attgatcagt cgcccatcgg
    20101 ccggacgccg cggtcgaacc cggcgacgta caccggcgtc tttaccccga tccgcgacct
    20161 gtacgccatg cttcccgaat cccgtgagcg aggctacaag ccgggccggt tcagcttcaa
    20221 tgtaaaggga gggcgatgtg aggcctgcca gggagacggg ctgaagcgca tcgagatgaa
    20281 cttcctgcca gacgtctatg tgacatgcga catctgccgg gcgaggcggt acaatctgga
    20341 gacgctccag gtaaagtatc gaaactattc catcgccgat ctgctcgagg ccaccgtcga
    20401 ggaggcgtca ggcgtgctgg agaacatccc gcagatccgc acgaagctgg aaacgcttcg
    20461 tgacgtgggg cttggctaca tccacctcgg acagccggcg accacgctat ccggcggcga
    20521 ggcccagcgg atcaagctgg ccaaagagtt gagccggcgc cagacaggcc gcacgttcta
    20581 catcctggac gagccgacga ccgggctgca cttcgacgac gtcaaaaaat tgctggatgt
    20641 gttgcagcgg cttgtcgatc tcggaaacac tgtggtagtg atcgaacaca atctggatgt
    20701 ggtgaaaacg gcggactgga tcatcgatct cggccccgag ggcggtgaag agggcgggca
    20761 ggtcgtggtg gcgggcacgc cggagcaagt ggttcgcagc cgcaagagct ataccgggca
    20821 ggctcttgtc aaagtgctgc ggaacggacg gaacgggtga gcaagtacgc aattcatccg
    20881 ctcgattttt cgggcctgaa gacaatcccc atccgggagc ggggaggcaa ggtgggagcc
    20941 gaggatttcg ccggtccgca cgtgcctggg accggcttcg gcgggtggct cgattcgttg
    21001 ccgaaggtcc tcgccgccga ttcgctccgc gcggtggtga gcgcgttgca ggcggcgagg
    21061 gcggcggcta aaccggtgat ctggggcatg ggggggcatg taatcaagtg cggcctcggc
    21121 ccggtgttga tcgacctgat gcgccgggga tatgccaccg gattcgctct gaacgggtcc
    21181 gcttcgattc atgacttcga gattgccatt gccggccgga cgagcgagga cgtcgaggcg
    21241 gtcctgcccg acggcagctt cggctccgcc gaggagaccg gccgcgagat gaatacggcg
    21301 atcgcggagg gtgaccgaaa ctctatcggc atcggggagg cgctcggacg gcgcatcgag
    21361 gtgctggcgg accccgcata ccagagttcc agcctgctct acgaagcatg ggcgcgcggg
    21421 gtgcctgtca ccgtgcatgt cgccatcggt actgacacgc cgcacacgca tccggctgtc
    21481 gacgcggccg cgatcggacg ggcgacccat cgcgatttcc ggctcttttg ctcgatggtg
    21541 gctgagctga acggcggcgg agtgtatctc aacgttggct cggccgtggt tctgcccgag
    21601 gttttcctga aggcggtgtc ggtggtgcgc aacctcggcc gcccactggc cacctttaac
    21661 acggtaaact tcgatttctt acagcattac cgaccgaagg tgaatgtcgt cgagaggccg
    21721 cactccggcg ccggggggca agggttcgcg atcaccggcc accacgagct gatgctgcct
    21781 ctgctggcgg ccatcctgat cgagttagac gcatgaacga gcacgagagc ataggccaaa
    21841 gcgcgatccc ggaagcgccg agtcgggccg aagagaaccc tttctggaac tatcaggacc
    21901 tggcgctgtt cctgacgtcc ggtatcccgg cgctgctggc cggcgcgctc atcgtcaagg
    21961 cgctgttcta tgggttttcg ctgagcgggc agggccaggc gcccgagttg ctgtcagcac
    22021 agttcctcgc gtatgcactc tggtacgtcg ttctgtacat gattctccac gtcaagtacg
    22081 ggcggccatt ctgggaatct ctcggttggg tccggccgtt ggagaggttt ggacggtacg
    22141 ccttgttggg tgtgttgttg gcattcgcgg tagcgattgc tggcctcttc ctcaaagcgc
    22201 ccgatattga aatgccgatg atgcggctcc tctcggaccg aacgtcgctg atactcgtcg
    22261 gactggcggc ggccacgctg ggtccgatat gcgaagaatt ggcgttccgt ggcttcattc
    22321 tgccgctgct cgtgcgttcc atgggcgtcg tgctcgggat tctcctcacc accttgccgt
    22381 tcgcgttgct gcacggcccg cagtacgcct ggtcatggcg ccatctgctg ttcatcgtac
    22441 tggccggcgc ttcgttcggt ctgctgcggc tgcgcacggg ttccacggcc gccgcgaccg
    22501 tcatgcacgc tgcgtacaac ctgacgtttt tcattgcctt cctcttccaa tcgaaaaact
    22561 tgcctgtgaa atgattgaaa cgattgtctg gactgacgcg ggggtcgtga tgatcgacca
    22621 gacccgcctg ccgaaggaag taacctacat gacgtgccgc gactaccgcg aggtggccgg
    22681 ggcgatccgc tcgatggtta tccgaggggc gcctgcaatc ggcgtggcag ccgctatggg
    22741 cgtagcgctg ggtgtgctgc gggctgatcc cggcaagctg gacgaggagt tcaagacgat
    22801 ctgcgacacc cttgcgggga cgcggcccac ggcggtcaac ctgttctggg cgatcgaccg
    22861 gatgaagcgg gtctatgagg gggtgcgcag ccggcccgtg gaggaggtgc gggcgcggct
    22921 ggtgacagag gcccagcaga tccgcatcga ggacatcgcc atcaacgagg cgatcggccg
    22981 caacggcgcg acattggtac cggatggcaa aacggtgctg acgcactgca acgccggcgc
    23041 tttggcaact gctggttacg gcaccgcgtt gggcgtaatc cgggctgccg tagccgaggg
    23101 caagaagatc gacgtgtttg ccgatgagac cagaccgttc ctgcaaggcg cccgcctgac
    23161 ggcatgggaa ctccagcagg acggcatccc gaccacgatc attactgaca atatggccgg
    23221 gcactttctg cgatcgggcc gggttgggtg cgtggttgtc ggcgcggacc ggatcgccgc
    23281 caacggcgac gtggcgaaca aagtgggcac ctacccgctg gcggtcctgg cgaaagagaa
    23341 cggtgtgccg ttttatgtag cagcgccggt ctcgacgctc gatctgagcc tcggctgcgg
    23401 cgatcagatc ccgatcgagc aacggccccc ggcggaggtg acccacgtgt tcggcgtggc
    23461 cgtggctccg gagggcatac gggtcgaaaa ccctgcgttc gacgttacgc cgaaccgtta
    23521 cgtctccgcg atcattacgg aacggggcat cgcacagccg ccatacaccg aatcgctcgg
    23581 gcgtctcatg ctagcttgaa tctatgcgta agtttgttct cttcgccgcc tgcgtgatgc
    23641 tttcggctgc gctgcacggg gccggcgagc tctccggccg gcgcgcaccg ggttttgcgt
    23701 tgccgaatat cgccgatccg aagctcggat accacgactt agcggattac cgcgggagga
    23761 tcgtgctgat cgagatcatg cagacgaact gcccgcactg ccgcgatttc tcgcgtgttc
    23821 tcgaagaagt gaaggccaag tacggaaacc gggtggcggt cctctcggtg gtaaatccgc
    23881 cggatacaca gcggaccgtc gcggcataca cggcagcgac aaagaccacg atgccgatcc
    23941 ttttcgactg cggccagatg gcggtgtcct acctgaaggt gactcctcaa aatccggcga
    24001 ttaacgtccc acacctgttc cttatcgacg gggaaggtgc gatccgcgac gactacggat
    24061 acagcccggc gaaccaggcg atcttcgagg ggaaggcact atttgcattg atcgacgcga
    24121 tgctcgcggg cggaaaaaag aaatagagcg ggtgagtgca ggctatcggg ttaacggggc
    24181 ggagattccg atggagagag ccaacagcag gatttccagc caacgatct
//
LOCUS       DATIFQ010000543        90842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_955371, whole genome shotgun sequence.
ACCESSION   DATIFQ010000543 DATIFQ010000000
VERSION     DATIFQ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 90842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90842
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955371"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..911)
                     /locus_tag="VLE22_27425"
     CDS             complement(87..911)
                     /locus_tag="VLE22_27425"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HSB18213.1"
                     /translation="MGVTQNERAARFRALHDGPGAFVIPNPWDVGSARILAGLGFQAL
                     ATSSAASASALGRRDGGLTRDEALAHARMIVDATDLPVSADLEKGFGDAPEVVAETIR
                     LAAGVGLVGCTIEDTTGNPDRPLYDTRLAVERIAAAAEAAHALPFPFILTARAHNLLF
                     AAPSLDDTISRLQAFEKVGADVLFAPGLPDLAAVRAVCAAVSKPVNFMVGIKGKSFSV
                     AELAAAGVRRISLATSLYRAAMTGFLDAARQVKDTGQFSFLDRCVTTLELNKLMGI"
     gene            complement(978..1766)
                     /gene="ygiD"
                     /locus_tag="VLE22_27430"
     CDS             complement(978..1766)
                     /gene="ygiD"
                     /locus_tag="VLE22_27430"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HSB18214.1"
                     /translation="MAETLPAIFFGHGNPMNALMDNGYTQGWRRIGGQTPTPKAILAI
                     SAHWYVPGTRVTIGTAPRTIHDFGGFPQELYEVQYPAPGDPQLARRVQELLAPLPVAL
                     DDDWGLDHGTWSILRHVYPQADVPVVQLSIDRMRPASFHYEIGQRLADLREEGILIAG
                     SGNVVHNLGAYAWGRPAEPYEWAMRFEGVAKELLLAGDDKSLIDYETMGRDAGLAIPT
                     PDHYLPLLYVIGARRPGDSITFPVEGIEGGSISMLAVQLGKSGA"
     gene            1865..2155
                     /locus_tag="VLE22_27435"
     CDS             1865..2155
                     /locus_tag="VLE22_27435"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSB18215.1"
                     /translation="MVTAIVLINTAQGRTPEVAQALIDLPGITEAYSVAGSYDLVAIA
                     RVRDHEGLADLVAGRVQKIPGIEATNTLIAFRAYSQRDLEAMWDIGNAGPIS"
     gene            2256..3443
                     /locus_tag="VLE22_27440"
     CDS             2256..3443
                     /locus_tag="VLE22_27440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB18216.1"
                     /translation="MAKAVSRTLPRGVIALGLVSMFMDVSSEMIHSLLPVFLVGVLGL
                     NALAVGLIEGIAESTAAITKIFAGVASDWIGKRKPLVLLGYGLAALTKPLFPLATGAG
                     AVLTARFLDRIGKGIRGAPRDALVADLTPKSMHGAAYGVRQSMDTAGAFAGPALAMVL
                     MALSGDNFRLVFWIAVLPAVISVACVVFGVQEPETHAVVGNRRFPLQRREIRRLSLKF
                     WLVVMFASMLTLARFSEAFLLLRAANVGLAVTYVPAVLIVMNVIYSASAYPFGHLSDR
                     LNRGSLLAVGIGFLIAADLVLAAANSTWAVLAGSMLWGLHMGATQGLLTTIVAATAPA
                     DLRATAFGLFNLVTGAALLLASGLAGFLWANYGPAVTFLAGAVFSTVALAGLLARRPR
                     SAP"
     gene            3513..5699
                     /locus_tag="VLE22_27445"
     CDS             3513..5699
                     /locus_tag="VLE22_27445"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSB18217.1"
                     /translation="MNVRIRMVTVFLLCVSSPLAGQGDPAVNRPKIGLVLGGGGARGL
                     AHIGVLEWLEEHRIPVDYIAGTSMGGIIGGLYAMGMKPAELRDLVNGIDWEETLRGTP
                     SYEQFLLRRKQDRRDYPGLQLGWNKGLRFGAGLNGGHFVSLILGRATLPYWQVRDFDE
                     LPIPFRCVAADLVSANAVVLRDGPVAEALRATMAIPGIFTPVHRGSALLIDGGVLNNV
                     PTDVAKDMGADVIIAVNAGTPLRTRKDLQSLIGILDQTTNLAAEQNVRKNLLLADVVL
                     TPDLGKYTAVDFDAREALAQAGYAAAAAKEPALRRFEIGRGEWERYSASVSGRVRREV
                     PTPQSLVVEAPTAGAANAIRERLGRHRGRTLNVDALEDDLTTMLGWGRYASLSYELRE
                     SSDPSRGPELVVRAEPFVNGTAVIRVGLSLQGAHTQQVEVNLRARDTVYDVGGYGSEM
                     RLDATLGTTLSSGVEYYRPLGKSRFFVAPRAYASRATTNVAEAKDVLANYRRSSAGAG
                     IDLGYGVLSRSDEVRFGYEVRYDDTSIVIGNPALPVYGGTANVLSLRWFHDGQDSATI
                     PRRGIQSTLRGSWFLNSATSARDFPQAEFTASAFHPVDAKGSVFATAAGGTTFNRIAP
                     LDQEFALGGPRQLSSYGFYELRGSDYALASAGYLRQISANSLAKLYVLGFLQAGRVFR
                     ARSADRTGMDVTAGVVADTFIGPILLGVAVGDAGHRKAFISIGRTF"
     gene            complement(5936..7282)
                     /locus_tag="VLE22_27450"
     CDS             complement(5936..7282)
                     /locus_tag="VLE22_27450"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HSB18218.1"
                     /translation="MVELNEHPTVQWLRERGQESAAAASNAGLDYQWLRQLCFQAGAD
                     DVGFVRIDHPELADQRQDILTLFPGTQTLVSLVCRMNREPIRSVARSVANVEFHRVTD
                     KINHAAARIVSTLEEKGIRALNPAAGFPMETSRFPGGKIWVVSHKPVAVAAGLGRMGI
                     HRNVIHPKFGSFILLATVLVDARVTEYNNPIDYNPCLECKLCVAACPTGAISADGNFD
                     FSACNTHNYREFLGGFTDWVETIAESGSAREYRQKVSGSESASMWQSLSFGANYKAAY
                     CLSVCPAGEDVIAPFLTDRPRFLREVVKPLQDKEETVHVVPGSDAEAYVKRRFPHKRT
                     KQVGNGLRVESVNAFLSGLPLVFQRGKSKGLNATYHFTFTGREERKATVVISGQRLRV
                     TEGLSGKCNLHVMADSLTWVRLLARDANPLWAIVRRKIRIKGSPRFLLAFRRCFPS"
     gene            complement(7337..7780)
                     /locus_tag="VLE22_27455"
     CDS             complement(7337..7780)
                     /locus_tag="VLE22_27455"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSB18219.1"
                     /translation="MPSEVKPLIALPCACANLRRASRVVTQVYDEELRAAGLKTTQFT
                     LLQALHLITPVTQGRLAELLGIDSTTLTRTLRPLIQKGWITSVPGSDKREHSLQLTSP
                     GRRKLKQAEPLWQQAQERLRKGLGDRDWENLRTITVRAAQAASAM"
     gene            complement(7865..10417)
                     /gene="ligD"
                     /locus_tag="VLE22_27460"
     CDS             complement(7865..10417)
                     /gene="ligD"
                     /locus_tag="VLE22_27460"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HSB18220.1"
                     /translation="MPKPLDEYSRKRSFEKTPEPKPDPESAPEGRVYCVQRHHARRLH
                     YDLRLQLGGTLKSWAVPQGPSLDPAIKRLAVMVEDHPLQYAVFEGTIPKGNYGAGSVM
                     LWDRGWWEPAESKSPEEMLEKGDFKFILHGEKLRGEFVLAHMKGAKGKGNDWLLIKKK
                     DAEARPGWDVEQYAYSVATGRTQEEIAQELPAREPEKKARDAPDGALSSPMPHSIQPM
                     LAVSAAEPPGSGEWLYEVKWDGVRALCFLNEGHLRIAGRKGTSIERQYPELGVLPHHV
                     AAESAILDGEIAAVDENGRPSFERIQPRIMARDANAVAQLARSRPVVFFAFDLLYWNG
                     FDLRGVPLKERRKLLEEVVRPGTAVRLSETFDVDPRQLLEAARAQGLEGIVAKRTASP
                     YQERRSADWVKVKATREQEFVLCGYTHGERDYFGALVLGVYEGGQLEFVGSVGTGFDR
                     KLMKQIRERLDELRTERSPFREKVSLPQEVQWVRPELVCTVRFLEWTNEGRVRAPVFV
                     GLRDDIDPSECTRSEPAAEEPAGKSAPLLPGDKAEATIEVEGHRLQIRNLNKVFFPGE
                     GITKREVLEYYNAVAELLIPYWKDRPLSLRRYPDGIAKEGFFQKDASGSGFPEWLRKE
                     RVLAEDGKMRLQVIGDSRAALVWLANLGCIDQNPWMSRVGSLDNPDFLLIDLDPQECG
                     YSRVTEAAQLIRRLLEESGLTGCPKTTGGKGMHIYVPLDPVYSYDHVRAFAEILARLA
                     ERERPDLFTLPRAVAKREKGKVYFDYLQIARGKTISAPYVLRAHPGAPVATPLDWSEV
                     REGLEPGLFHIRNALTRFERAGDLFEPVLKLKQRLEPALERLEKRTERGRKP"
     gene            10519..11352
                     /locus_tag="VLE22_27465"
     CDS             10519..11352
                     /locus_tag="VLE22_27465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HSB18221.1"
                     /translation="MASSVWKGHITFGLISLPVRLTAAARSETVSFNQLHKQDNSRVK
                     QVLFCIAEDKPVPRNELVKGYEYEDGKYVVIDEEDIKKIQPKTAKVMEILEFVDARDV
                     DAVYLESSYYLHPEEAGEKAYTVLFEALKRTGRAGVAKVTMHNREHVVILRPGKRGLM
                     LHTMYYQDEVRASDEFRTDGSIVKDPEVAMAQQLIEALVVPFDPSKFSDGYRETLRAM
                     IDAKISGEEVVKAPPAQELAPVIDIMEALKNSLVALRKPPAVETVTEITAKPKRRRAA
                     G"
     gene            complement(11399..12313)
                     /locus_tag="VLE22_27470"
     CDS             complement(11399..12313)
                     /locus_tag="VLE22_27470"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSB18222.1"
                     /translation="MSLRPIRRLSKAKPAIEGAGVHLRRAFGFGNTSDFDPFLLLDDF
                     RNDVPEDYLAGFPWHPHRGIETITYVLAGTVEHGDSMGNQGVIGAGDVQWMTAGRGII
                     HQEMPKGDRVGRMHGFQLWANLPAALKMTAPRYQEVKAADIPEVKDDDGTRVRIVCGS
                     FWGTKGPVDGVAADPLYLDVSVPPGRTKTLPVETTRNVFAYVFSGAGKFCNASGPLAV
                     PTEGVGWADTAPPTEADNRSLVLFDNGDQLTVRAGDDGIRFLLVSGKPIAEPVAWYGP
                     IVMNTQEQLRQAFEELERGTFLSEHGSD"
     gene            12541..13089
                     /locus_tag="VLE22_27475"
     CDS             12541..13089
                     /locus_tag="VLE22_27475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18223.1"
                     /translation="MTRNAQSAVLILGCCTALLLAGQGKGGGQGRRMGGPPSGAPQSR
                     GQGQQAHGANPAKSGGQGAHGSGQSAVEHLQRHPEQAARVQQMLPPGTDMNSAADGFK
                     SFGRFVSAVQVSKNLNIPFDQLKMKTTGPEAKSLGDAIHELRPDLSRDKVKDAIKAAR
                     QEERQIRRESRREVIGRNPTKS"
     gene            complement(13169..13480)
                     /locus_tag="VLE22_27480"
     CDS             complement(13169..13480)
                     /locus_tag="VLE22_27480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18224.1"
                     /translation="MKKLLLTLAVAALSIASAETFKVNLYQASYLGDKELAAGAYKLE
                     VTEGKVVFHRGKESAEAQVKAESAGSKFKTTFIRYSNGDGKFRIQEICLGGTDTKLVV
                     N"
     gene            complement(13572..14504)
                     /gene="miaA"
                     /locus_tag="VLE22_27485"
     CDS             complement(13572..14504)
                     /gene="miaA"
                     /locus_tag="VLE22_27485"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSB18225.1"
                     /translation="MATADPPLVVVAGPTGSGKSDLALRIAEDFCGEVVNCDSLQIYR
                     YFDIGTAKLPEFERRGIPHHLIDIVDPGEHFSAGEYARRARRVISEIAARKRLPVVAG
                     GTGFYVRALLDGLFPGPARDDTVRARLEAREARRPGGLHRLLRRFDPEAARRIHARDT
                     HKLVRAVEVCLLLRRPLTDAFAAGRDPLTGFHVLKIALDPPRQALYERLDRRCERMFE
                     TGLVDEVNRILDLGFSPEVKPLESHGYKQALQHIRGELSLPEAVLQAQRNTRRYAKRQ
                     WTWFRREPGIQRFGGFGDDPDVQAAVLEQIRKQM"
     gene            complement(14522..16369)
                     /locus_tag="VLE22_27490"
     CDS             complement(14522..16369)
                     /locus_tag="VLE22_27490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18226.1"
                     /translation="MTKAAIRCRIALVLLMIHPAFAAAPTRSTGAELPDRYFRLLDAG
                     VAHVEKQLSEEQSLDFEKLERRGDGWRLFPHVILAAAVLYAKQDPANRQYHDPKMLAL
                     AIRIGDRMASDSELGTFEKRLNSDRDAYMWLESYRLLERELGEERRKRWRRELERNLA
                     ELAADSAARIDFPGYNSPFNGTSPNHLSLWASTIHLAGKMFQNKEWEKIGAAVMHRFA
                     ALEQSPDGFWGEHEATLPTPGYDYTTYTGVALYAEHSDDPAALEALRRGLDFHKYFTY
                     PDGAPVDVLDDRNRYTIVPGWNVPGFSTWLDGAPSPGGNDESASKGHFGFSRFPDGRR
                     YAEFLTSFFREGEVGYEDLGRLAQNALYYHRGTTAPIPQDLSNYSRRLSVPAGIRKTG
                     PWVVCLSGIISTQAVNNQFYLDRQGHVSVFHDKLGLIITGAGSKNQPELATFFEKLLG
                     RFFHMPVSSRLRMEEARDRLSLAYNTFFADLYVPPPTRKGLILRFVISGKGTPAEDPR
                     LTLQLRLKAGETLETGAGKSIRLGAERIELSQAEIGGWIRHHGWTLKVDPAARLAWPV
                     YPFDPYANAPESSVEHAVGALSVPLRLVPQRGRYVRPAEQEILFELGID"
     gene            complement(16436..18307)
                     /gene="selB"
                     /locus_tag="VLE22_27495"
     CDS             complement(16436..18307)
                     /gene="selB"
                     /locus_tag="VLE22_27495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HSB18227.1"
                     /translation="MRNIIVGTAGHIDHGKTALVRALTGIDTDRLDEEKRRGISIDLG
                     FAHMDLPPDLHIGFVDVPGHERFVKNMLAGIGGIDLVLFVIAADESIKPQTREHFDIC
                     RLLGIPRGVVVLTKSDLVDRDILDIVHLEVEEFVAGSFLEGMPIVAVSSTTGDSLDQL
                     RARLAEIAAAAPPKDAGRHFRLPVDRAFSMRGFGAVVTGTLISGSVAREQEVELYPIG
                     RRCRVRGVQVHGGQVERAIAGQRTALNLSGVEPSEVTRGMVLSEPSRFQPGAVIDCRF
                     ELLPSAKALKHRAPVHFHAGTAEIEAEFRLLDGSTALQPGGRTFARIVLRDPVLLLPG
                     DRFIVRMFSPVVTIGGGVVVDTGARPYRRHEDAAARLRVLAEGTPAERIALLVRESRF
                     GVGLAELIARTGLLAEEVDAAVRSANFIRLDQPDPWLVDSEWFQQATARIQSVLSEHH
                     RANPLLPGLPRHELRSRELPDAPLFLLEALLAASPLVVAEGEVVRLKSHRLVLKQDEE
                     QAVKAIESLFAEAGLTVPPVNEVLAKSGVEAGRARSLLQILLRQKRLVRVGDELVFHA
                     DALRSLREQLAARRGQRFTVPVFKEWTGISRKYAIPLLEYLDRERVTRREGDERIVL"
     gene            18306..19223
                     /locus_tag="VLE22_27500"
     CDS             18306..19223
                     /locus_tag="VLE22_27500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSB18228.1"
                     /translation="MGLLLILLALAAFAQEQETPVFRSDVTMVRVDAQVVDGKRLVAG
                     LGREDFEVFDEGQRQEIAYFGRESEPLDVLLLLDVSGSMRRYVEEMARVARDALRQLR
                     AADRVAVMLFSRRSKVSEEFTADFTEVAREMSAAVREQGLGAGTAINEALLAASAYAG
                     RQPARPGRRAILILTDNLGLNYQTPDEQVVRALYEADCVLNAIVARGGERPKPPRPGE
                     YLNPDFTPSDVFRLAEETGGEAVKAERAGAAFQEMMERLRTRYSIHYRAPEAPAGSFR
                     RIRVALTEAARKRLPRAEVRARAGYYASR"
     gene            19239..20780
                     /locus_tag="VLE22_27505"
     CDS             19239..20780
                     /locus_tag="VLE22_27505"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HSB18229.1"
                     /translation="MDRLEEVRRRHGAAEAGGGEERRERQHQAGKLSARERIDLLVDE
                     GSFEELDKLVRHRGRDFGMDEQVIDGDGFVTGYGRIDGRLVYVFAQDFTVFGGSLSEA
                     NAQKICKVMDQALKNGAPIIGLNDSGGARIQEGVLSLGGYADIFLRNTLASGVVPQIS
                     AIMGPCAGGAVYSPAITDFVFMVDKTSYMFVTGPDVIKTVTHEDVTKEKLGGSVTHNT
                     ISGVGHFIAADDAECLRMIRELLLYLPQNNHDDPPAVPTQDPPEREDPTLDAIVPAES
                     NVPYDIKDVIHRVVDDGAFLEVHEHFAKNIVVGFARMEGRTVGIVANQPAFLAGCLDI
                     NSSVKGARFVRFCDAFHIPILTFEDVPGFLPGTDQEFGGIIRHGAKLLYAYAEATVPK
                     ITVITRKAYGGAYCVMGSKHLRTDINFAWPSAEIAVMGPEGAVNIVYRRELAAAEDEA
                     AVRREKVAEFRERFANPFIAAERGFVDDVIEPRQTRPKVIRALRMLENKVDTMPRKKH
                     GNIPL"
     gene            complement(20899..21036)
                     /locus_tag="VLE22_27510"
     CDS             complement(20899..21036)
                     /locus_tag="VLE22_27510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18230.1"
                     /translation="MRRVSIRELHLNTGAIVKEASEGEVIVVEWSPDGGIASRLFDSA
                     R"
     gene            21118..21342
                     /locus_tag="VLE22_27515"
     CDS             21118..21342
                     /locus_tag="VLE22_27515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18231.1"
                     /translation="MLPLKVTTVGKSAGFILPKEAMTRMKLKKGDIVFLTETREGYLL
                     TPYDPEFEAQMEIAQEGMRRYRNALRELAR"
     gene            21339..21764
                     /locus_tag="VLE22_27520"
     CDS             21339..21764
                     /locus_tag="VLE22_27520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HSB18232.1"
                     /translation="MSKEPVWLDHRLVLAIHGRVLVEHGGRAGVRDEGLLESALARPR
                     NLRHYQRASLYELAAAYAAGICRNHPLVDGNKRVALMAVYVFLARNGVVLRAGEADAA
                     AAVTGLAAGTLPESQFSAWLKKNTERPARPVKRVPGGRE"
     gene            complement(21769..23577)
                     /gene="lepA"
                     /locus_tag="VLE22_27525"
     CDS             complement(21769..23577)
                     /gene="lepA"
                     /locus_tag="VLE22_27525"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSB18233.1"
                     /translation="MDPRYIRNFSIIAHIDHGKSTLADRLLELTGALSEREMMEQVLD
                     SMDLERERGITIKAHAVRLNYIADDGNHYQLNLIDTPGHVDFTYEVSRSLQSCEGALL
                     VVDASQGVEAQTLANTYLALHHNLEIIPVINKIDLPSAEPERVKEEIEHVIGLDTRDA
                     VMASAKNGVGIHETIEAIVHLVPPPRGNPEAPLRALIFDSWFDPYRGVIALLRVVDGA
                     LRVGQKIRLWSSGKMFEVEGLGYQTPKRIPCDELTAGEVGFMSANVKTVSDAQVGDTV
                     IDDANPAPDPLPGFQEAKPMVFAGLYPVESHAHGLLREALEKLRLNDSAFSFEPESSV
                     ALGFGFRCGFLGLLHLEIVQERLEREFEIDLITTAPGVRYRVVKTDGGVVEVDNPTRF
                     PNPSLIETTEEPIIEATVITREESLGEILKLLEEKRGTQKKFEYIGVGRVLLMYELPL
                     NEIVLDFYDRLKSVSRGYASLDYHLAGYRESPMVKLDVLVAGDPVDALSMIVHRDFAY
                     ERGKALIERLRKLIPRQMFEVALQAAIGNKIVARENIPAMRKNVLAKCYGGDITRKRK
                     LLEKQKEGKRRMKRVGKVDIPQEAFLAVLRVGGDSE"
     gene            23614..24399
                     /locus_tag="VLE22_27530"
     CDS             23614..24399
                     /locus_tag="VLE22_27530"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSB18234.1"
                     /translation="MSYGSIAFELSDDGIALITIRRPEKLNALDVATVGELDDALSRA
                     ASDASTRGLLLAGAGDKAFVAGADVRELAALDAAGCREFSLRGQRTFRRLETMGKPSV
                     AAIHGYALGGGLELAMCCTFRVAAPEAKLGLPEVKLGIIPGYGGTQRLPRLVGRSRAL
                     GMLLTGEPVGAEEALRIGLVDHVAPRAELTEFCRGLLRKMLANAPVAVALAMEAVDIG
                     LNAGVEEGLRAEAAAFGLASATEDRAEGMRAFLEKRPAVFRGK"
     gene            24403..24882
                     /gene="ribE"
                     /locus_tag="VLE22_27535"
     CDS             24403..24882
                     /gene="ribE"
                     /locus_tag="VLE22_27535"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF000812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HSB18235.1"
                     /translation="MASRIIEGNLDAKGLKFAVLVTRFNSLITERLLSGALDALARTG
                     CGADDIEIVRVPGSWELPVVARELALLKRHDAIICLSAVIRGDTPHFDYIAAEASKGL
                     AQTSMENGVPLAFGVLTCDTLEQALDRAGAKSGNKGFDAAMTAVEMGNLMRRLRAGS"
     gene            24890..25360
                     /gene="nusB"
                     /locus_tag="VLE22_27540"
     CDS             24890..25360
                     /gene="nusB"
                     /locus_tag="VLE22_27540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSB18236.1"
                     /translation="MPSRHRSRQRALQVLFQIDIRQQSAAEALDGFYGSLITSEEAPV
                     ESEERDPFMERLVKSTVERLSDIDRRVSGHSEHWRIERMAAVDRNILRLAVCEMTSTD
                     TPAAVVIDEALELARRFSSDESVAFINGVLDAVHRSWTSGHEVTGPPGPAPKSQ"
     gene            complement(25323..25526)
                     /locus_tag="VLE22_27545"
     CDS             complement(25323..25526)
                     /locus_tag="VLE22_27545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18237.1"
                     /translation="MEGRKTFLLRIDGRTFDLLRRWADDEMRSVNGQIEFLLRDALRR
                     AGRLTESAASGFTGFSEQDPEGR"
     gene            complement(25533..26414)
                     /locus_tag="VLE22_27550"
     CDS             complement(25533..26414)
                     /locus_tag="VLE22_27550"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HSB18238.1"
                     /translation="MIKEIPRSTLPGVFTLIALIVVAALCVYGIIDTARTTPRGEQPW
                     LIILYIVVLVVNSLCFAGLFMVQPNQGAVLQLFGKYVGTVRRSGLHWANPFFSKKHIS
                     VRVRNFETGHLKVNDKDGNPVEIAAVVVWKVVDTAEACFQVDNYENYVHVQSEAALRN
                     LTTAYPYDSHEETETSLRGHTQAVAEHLKTEIQERLAVAGVEVVEARISHLAYAPEIA
                     AAMLQRQQAGAIIAARQRIVEGAVGMVEMALEMLSRNKIIELDEERRASMVSNLLVVL
                     CGERAAQPVINAGTIYQ"
     gene            complement(26521..27069)
                     /locus_tag="VLE22_27555"
     CDS             complement(26521..27069)
                     /locus_tag="VLE22_27555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18239.1"
                     /translation="MDRKTFLRNLAAGAVLPCCSSLGQTVSGKCEDAKCTSDAKAVRD
                     FLSTFLQKEESSLDRDTLIKLMTERGRACCRALDFRQKLIQDSQGNLDKLVELLGKIV
                     GPENCRREGDLVTLIYPSGQCVCGWSPKRAPTPDDPYCDCSKANNQLLFETVGGKPVR
                     IEVADSPRRHAGARCRFLIDLS"
     gene            complement(27171..28523)
                     /gene="mnmE"
                     /locus_tag="VLE22_27560"
     CDS             complement(27171..28523)
                     /gene="mnmE"
                     /locus_tag="VLE22_27560"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HSB18240.1"
                     /translation="MNFRDTIVAISTPPGRGGIGVVRLSGPGAREIAARILRFQDGPH
                     WRTWTSALAELADEAGHTVDQAVVTFFEQPRSYTAEDVVEISLHGSPVVLRFCLEKAC
                     DAGARLAEPGEFTLRAFVHGRIDLPQAEAVRDLIEATTLYQARIAAQQIGGSVSRRVA
                     PVKEQLLELIALLEAGIDFAEDDVAVAPAEEILRRIDGIRDAAGRLLSSFGYGRLVHS
                     GLTLAIVGRPNVGKSSLFNRLVEQDRAIVTEIPGTTRDLVSEVASLGGIPVKYVDTAG
                     IREGEGLVESLGIERSFQAMADADLTIVVLDLSVVVETQDLELVRRASGQGRFIVAGN
                     KCDLPRAAGMDIDTVVVSALTGEGIDELRRRILDALAPEGGDLADSGFITSIRHERLL
                     RETTASLDKAHTAGLGGIPHEMLLLDLYGALRPLDEMTGATTADDILNRIFSTFCIGK
                     "
     gene            complement(28525..29097)
                     /locus_tag="VLE22_27565"
     CDS             complement(28525..29097)
                     /locus_tag="VLE22_27565"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HSB18241.1"
                     /translation="MGTRKYTTESLAPRVEAFLQPVLKKAGFHLKFEVSDYDGQYAEI
                     EDPDVVVRFSGSDVDDLLANRAELLLALEYLTMEILRVPPEDHSRLRFDANDYRLLRI
                     EELRLSAAAAADKVRRTGQSFRFSPMTSRERRILHLAFRDEQGVRSESAGEGPRRQVV
                     VYPEGAPSEPPDQFHAALPGRPPGVRRRRR"
     gene            complement(29101..30840)
                     /gene="yidC"
                     /locus_tag="VLE22_27570"
     CDS             complement(29101..30840)
                     /gene="yidC"
                     /locus_tag="VLE22_27570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HSB18242.1"
                     /translation="MDDPENGAKTGGKPGGQKGLSMERRMLLAFLLMGLVLMLTPYFY
                     RSSPPSSKAPVKPAASEDSPAKPSAKPPVEQAATAPEAPLPAVPAGQVGAEKEETYIV
                     ETDLARVVFSNKGAVVRSWILKKYKDGAGKPLDLVHAAGAARAGYPFSLVFKGKAAAD
                     LKQVLYAAKPVSDGLGVDFEFSNGRVWSRKSFRFRKDSYLADVSSEVTQDGKSLPHYI
                     AWRGGFGDGTVPNHPATEHTLHFDVTANKLIVKEAKAASDGPLSAIGTFSFAGIEDQY
                     FAAVFLPASGVSTEVFTYQDHVAGEQKDKEVPHIGMSVGGSGANRMPIFVGPKDIDIL
                     RKVNPKLEQVVDFGWFSFLAKPLFLAVNWVNDRWVQNYGWSIVVVTIIINFMLLPLKF
                     TSLKSMKKMQMLQPQIAAINEKYKGIGIRDPRKADQNQEVMELYKKHGVNPMGGCVPM
                     LLQIPFFIAFYKVLTVAIEMRGASWLWVSDLSQPETLPIRILPIAMIVSQFIMQKMTP
                     STTADPSQQRIMLMMPLILGFMFYGVSSGLVLYWLTGNVVGIAQQWFFNKTSVAPVPP
                     PPPVKQVKKGAKK"
     gene            complement(30883..31092)
                     /gene="yidD"
                     /locus_tag="VLE22_27575"
     CDS             complement(30883..31092)
                     /gene="yidD"
                     /locus_tag="VLE22_27575"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HSB18243.1"
                     /translation="MQALVVFLLRFYKRCLSPLLPSACRFYPTCSDYMRAAVEKYGAR
                     RGVWLGLKRLVRCHPFHAGGFDPVR"
     gene            complement(31080..31367)
                     /gene="rnpA"
                     /locus_tag="VLE22_27580"
     CDS             complement(31080..31367)
                     /gene="rnpA"
                     /locus_tag="VLE22_27580"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HSB18244.1"
                     /translation="MYDQGFRVAGPFFAAFCLNASGDERSRVGFTTPRALGKSTGRNR
                     IKRRMREAVRLSLELLGPGWWIVINPRKAAQEASFEDLKREVERLFLRCRR"
     gene            31784..35047
                     /locus_tag="VLE22_27585"
     CDS             31784..35047
                     /locus_tag="VLE22_27585"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSB18245.1"
                     /translation="MRYRRAAFLLLILLIAADFSALLAQRGFDEYALVLQDPPLAAQV
                     PSRKELRRAAADDGLRRIEAAQSQMREELGRRGIHVSGSARILVNAVFVRAPKEKLAE
                     LRALPGVSRLEYMPPLRPLLDRAAELINAPAAWNALGGTANAGAGIKIGILDTGIDQN
                     HPGFRDPSLTAPAGFPKCQPQDCAYTNSKVIVARSYVSMLAAGFGDDVAATSRPDDLS
                     PRDRVGHGTAAAMIAAGVRNTGPSATITGIAPKAYLGNYKIFGSPGVNDYTFSSIVIQ
                     ALDDAFLDGMDIVTLSLGSPAGYGPLDYPCTSSTGQPQPCDLRADAVQNAVNKGMVVV
                     AAAGNDGDIGLQFPTLSTIATPGTAPAAITVGASTNSHVFFARVKLTGGDAPQNLQQI
                     PALFGDGPHPLAALTAPLKDVSKLGADGRACNPLPGGSLASSIALVERGDCSFTLKVN
                     NAQTAGATGVVIYNNVDSNYPPFSPVGLVGTGIPAAIIGRNPGLALKDFLVAHADRPT
                     TMDPALVPQDADYDTVAFFSSHGPAIGTNGIKPELVAVGTDLYTATQTYDPNSGLWDP
                     TGYTPMQGTSFSVPMVAGAAALVMQARPSAQADDVKSAVVNTAMNIIQDDVGLARVTA
                     VGAGILDANAAVRSTVSVDPATLSFGVIAGSLPSTTLRITNTGMQPVNLTLAVEPRDT
                     DANARVVLDRSSLPLAAGATTTVVARLEGTQPGPGSYEGAVTITGGSVALRVPYLYLV
                     GDGIPYNMFVMFGDEFDGTPGGELIGRLIGFKLIDKFGVPVASVPVVFRSTLGGGTIK
                     SADANTDIYGIAAAIPELGPQIGQQQFTATAGGLTVDFNGEAFPSPSIYTNGIVNAAS
                     GRIDQGLAPGSYVSIYGQALSPSFLAFDSLPLGYLPLSLTNVSVSFDVPDRGISLPGR
                     LHFVSDGQVNVQVPWELEGLNSVKVKVSIADISSAVYDMPLARNSPGFFEYPLGSGTV
                     AALVSRNGQYSVITTSNPARRGETVELYCNGLGPVNDTPPTGEVTPAGVLRRTRDTVS
                     VTIGGRPATPDFSGLTPYSAGLYQVNVKVPDDAGVGNRPVVMSVNGVTAKTVNLPVQ"
     gene            35097..35567
                     /gene="greA"
                     /locus_tag="VLE22_27590"
     CDS             35097..35567
                     /gene="greA"
                     /locus_tag="VLE22_27590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HSB18246.1"
                     /translation="MEEVKKKLQEEIAALEYELRNELPREILKARAHGDLSENAEYHA
                     AKERQRYVSARLSQLQKRLGELSMVDLRNLPQGRVGLGSKVVVLDLHKDREIAFKLVT
                     SEEADASKGWISTSSPIGKGLLGKELGDTVKVQIPGGVRELEIVKLTTVHDADE"
     gene            35592..36230
                     /locus_tag="VLE22_27595"
     CDS             35592..36230
                     /locus_tag="VLE22_27595"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSB18247.1"
                     /translation="MTYTRAIGVACNKVIRLMVRGLALARIHPNVLTFIGLLINGVAG
                     VLLASGRFVAAGLVIIIAGIFDMVDGRVARETNQVTAFGGFFDSVLDRYSDLVLLMGM
                     LVYYASIERFFYVVLTAVVMTGSVMVSYTRARAENAIPRCKVGFMERPERIVLLIIGA
                     LFNRMAQVLWVIAVLSNLTVIHRLIFTWQETKRLEEAQLQPTKSTEMAERKA"
     gene            complement(36236..36616)
                     /locus_tag="VLE22_27600"
     CDS             complement(36236..36616)
                     /locus_tag="VLE22_27600"
                     /inference="COORDINATES: protein motif:HMM:NF011254.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-[acyl-carrier-protein] synthase"
                     /protein_id="HSB18248.1"
                     /translation="MIAGTGLDVAEVDRIRAAIERFGRRFIERIYTPGEIAYVERKAN
                     RFERYAARFAAKEAGMKAIGTGWRHGVRWRDFEVANLPSGKPTLLLHGVAARFAERLG
                     VKSVSLSITHTAAVAVAFVIMEGG"
     gene            complement(36613..37029)
                     /gene="nikR"
                     /locus_tag="VLE22_27605"
     CDS             complement(36613..37029)
                     /gene="nikR"
                     /locus_tag="VLE22_27605"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HSB18249.1"
                     /translation="MGELSRIGVAIDTDLLEKFDALILSRGYTNRSEAFRDLIRDELV
                     EKAWQAPDSPVIGTVTLVYDHHVRLLNEKLTDLQHKYFDHILSALHVHLDHDNCLEVL
                     VVKGNASVVRKIADSLISTKGVKHGRLTITGSGAVL"
     gene            37114..38022
                     /locus_tag="VLE22_27610"
     CDS             37114..38022
                     /locus_tag="VLE22_27610"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HSB18250.1"
                     /translation="MHIPDGFLSTSVWATMSAATVPSVAYMVRRAQREVEEARVPLLG
                     VMGAFVFAAQMINFPVGIGTTGHLIGSAVLAYTLGPAAASVVMTAILAIQALVFQDGG
                     LLALGANVFNMAVAGVLAAYLPYHYWGAGRWRKAAIFSGAALSVLTGALLALAQLRFS
                     GVAMPGAVVWVSLGLFTVNALLEGGITLAVMQSLEALNPGWLRKPAASGRTAMGVLLT
                     AAVLLVGAGVLFAASDPDGIEKLAENLGIASHARTLLETPLGGYEIQYLSSGWLRKAT
                     AGLAGLALIYGACVVFGKLIARRRSA"
     gene            38023..38766
                     /locus_tag="VLE22_27615"
     CDS             38023..38766
                     /locus_tag="VLE22_27615"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HSB18251.1"
                     /translation="MHHVVLDRWSRGRSFLHHRDARAKVLALLAFLIIVATTPPGSNA
                     AAAGYGAFLLAAALLAGLPAGGVLWRGAVVLPFSAAFAAMSLFAGDNIRAIALLEKSY
                     LSALAVVIVVGTTPMPELLRGLESLGVPRFLLLVVQFLYRYLFVISEQAQHMRVAAGA
                     RGTASTLAVFRRWRLRAAAGAISVLFARSYSRAEGIYRAMAARGFRGHISLLAAKRFR
                     WQDVAFLVASVVVPAAVRIALGAGGGWPR"
     gene            38754..39473
                     /locus_tag="VLE22_27620"
     CDS             38754..39473
                     /locus_tag="VLE22_27620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB18252.1"
                     /translation="MAPLIEVKDLRFRYPDGTPALNGIDFRLYLGETVALLGANGSGK
                     TTFVLHLNGILQGEGLVSVCGMQLSKETLPDIRRKVGLVFQEADEQLFMPTVLDDVTF
                     GLLNLGISPEEAATQGREALARVGMEGAGGKAPYHLSAGEKRRVALAGVLVMRPEILI
                     LDEPTTYLDPPGQRNLLELLRELPQAKIVVTHSTTLALAVSQRAVFFQKGKIAGEGVT
                     EEIIQRFQWDARQPCRGIHGG"
     gene            complement(39492..39764)
                     /locus_tag="VLE22_27625"
     CDS             complement(39492..39764)
                     /locus_tag="VLE22_27625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18253.1"
                     /translation="MARVTKALKDTSIYAAALEGLELQKQRIEEQIQQVRALLGARRV
                     KSNSPSGPAAEKPARKRRLSAAARKRIAQAQKRRWAEYRKAQAASA"
     gene            complement(39810..40259)
                     /gene="dtd"
                     /locus_tag="VLE22_27630"
     CDS             complement(39810..40259)
                     /gene="dtd"
                     /locus_tag="VLE22_27630"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HSB18254.1"
                     /translation="MRVVLQRVSEARVEVGERVAGSIERGLVALVGIARTDVRADADY
                     LADKTVCLRIFPDEEGKMNRSLLDIGGSMLVVSQFTLYGDVRKGRRPGFDRAAAPELA
                     RNLYEYFIDSVRTRGVSVQSGVFQAEMAVHLVNDGPVTFILDSEKSV"
     gene            40287..41279
                     /locus_tag="VLE22_27635"
     CDS             40287..41279
                     /locus_tag="VLE22_27635"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18255.1"
                     /translation="MIETYAYARAGLLGNPSDGYFGKTISLLVKNFRARVLLYPSARL
                     EIRPSKADTPIFENLQDLYSTTRWRGYYGGIRIIQALVVRFIDYCREVNFPLEDRNFT
                     LEYESSIPLRLGLGGSSAIITAALRALCQYFGIEIPKPVQANLVLETETKELGVPAGH
                     QDRVIQVYEGLVYMDFARELMETRGYGNYENLDPGLLPSIYVAYRTSLSEGTEIFHSS
                     VRERWRRGDPEVVEAMRAWASYAQEGREALLRRDGDTLAGLIDANFDLRARLYDISDG
                     NLRMIRVAREVGATSNFAGSGGAIVGTYRDERMYKSLVGAMKEIGVAVIKPRVT"
     gene            41357..42403
                     /gene="purM"
                     /locus_tag="VLE22_27640"
     CDS             41357..42403
                     /gene="purM"
                     /locus_tag="VLE22_27640"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HSB18256.1"
                     /translation="MAARKIIQYKDVGVNIDEADRAVAQIRVMARKTFTRGVLTDIGS
                     FGAGYRLAGWKQPVLISSADGVGTKVKLAFLTGRHNTVGEDLVNHCVNDVAVQGATPL
                     FFLDYFAVGRLDAGVAAQVVSGLARGCEANGCALIGGETAEMPGLYAPGEYDLAGFIV
                     GAVERVRLVTGRDIRAGDVLLGLHSTGLHTNGYSLARKVLLEVAEYQPHTFLPELGRT
                     VGDELLQVHRSYLRPLQALFTKGLLKGAAHITGGGITENTPRILPKGLAAEIDTARWR
                     VPTVFELLRRIGNIPDDDYRRTFNLGIGMILAVSTRKAAEAKRTLDRLGEEHVEVGRV
                     VPLPRGAKGRVLYI"
     gene            42400..42999
                     /gene="purN"
                     /locus_tag="VLE22_27645"
     CDS             42400..42999
                     /gene="purN"
                     /locus_tag="VLE22_27645"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HSB18257.1"
                     /translation="MKRLGILLSGRGSNFEAIADSVVTGRIDAEIAVVLSNRPEARGL
                     EIARSRGLNASCIPSKGLDREVYDRMVVEELKKSRVDLVCLAGFMRLLSAYFIREFPL
                     RILNIHPSLLPAFPGLDAQHQALEHGVKLSGCTVHFVDENLDSGPILIQTAVPVLDDD
                     TADSLSARILKQEHRIYTEAIRLVLSGKFRIEGRRVIAG"
     gene            43243..44415
                     /locus_tag="VLE22_27650"
     CDS             43243..44415
                     /locus_tag="VLE22_27650"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB18258.1"
                     /translation="MRSMTTGILAAALLLAANATSGTGDDRPAAGQSVKPENLHGISA
                     SSAAAKSLNDLGALHYRQGRYEQAEEAYKKAITIWEQGPERESPEAASTIDNLGVLYR
                     AQARLGEAAELHRRSLAMRERLLGPEHGDVAVSLANLAEVVRMQGHAGDAEPLARRAL
                     AIAERAFGPEHAKTGFALAVLGAIYREQQRWPEAETVYERAAAVSAASLGENSAAVAE
                     NLNNLATVYIAQRRFTEAEAQLRWSLEVWERSLGPAHPSVAVALNNLAQVERLQGRYG
                     EAEPLYRRALEILQRTNGRSHPSVAKGLRNLGALFFEQGKLAGAAELYRQSLEISEGL
                     EGPEQPEVEACREDLARVYRAQGRMSEAKALWRGSLGAPNSLREADSGEQRLKAGR"
     gene            complement(44347..46179)
                     /locus_tag="VLE22_27655"
     CDS             complement(44347..46179)
                     /locus_tag="VLE22_27655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB18259.1"
                     /translation="MDEPSVGAAAARRPAAGVSPKAVRAQLERILHSAAFVRSGRISR
                     FLRFAVDQALDGRSDELKESLLGVEVFDREPSYDPRTDPIVRVEARRLRSKLTAYYER
                     EGRHDDIVIEIQPGGYVPRFARRAGSTLDPDRSPPESPAGDVPQAIVVLPFTNLTPDQ
                     ENEYFSDGLTEELIHRFTRIAGLRVVAWQSASQFKGKSYDLKQLGEQLKVDAAVGGSV
                     RRSGDRLRVAAHLVSTSDGRYLWSEAWERKLQDVFAIQDEISRSIADTLRLKLVEQPA
                     SVSERRASADVAAYNLYLLGRFHWHKRTPDGLRQSIRLFEQAISADPALALGYAGLAD
                     AYSLLADYGLAQPSEIMKKARRAARRALAIDPTLAEAHTSLGFIRSLHDWRWDAAEKH
                     YRKAIALNPGYATAHHWYAIDFLAMQARFEEARREMDIALRLDPLSLIINEGVGYLAL
                     LGRRYDEAIECYEKALELDRNFYKAYTAIGRALSLQGKYREAAEMFEKGRSLGGDVPS
                     ILAALGHNRSLAGDRAGAVALLEELRAMSGQRYAPTTCFALIHVGLGEKERALDWLEL
                     GCERRELPLANIGVHPIYDELRSAPRFQALLARIGLPKRVRGPQ"
     gene            46258..47436
                     /locus_tag="VLE22_27660"
     CDS             46258..47436
                     /locus_tag="VLE22_27660"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB18260.1"
                     /translation="MQRRTFLAATAASYAQILGANDNIRIGVIGSGGRGRLLTSEFKE
                     VGAEMAAVCDVYEPHLQAGVKAASTGATAYTDYRRLLDDKSIHGVIVATPDHWHAQMA
                     IDAVNAGKDVYLEKPMAHTIDEGFRIVEAVRKTKRVVQVGMQRRSYDVFHNAHSIMKS
                     GQVGEVRLVNSWWMNYTPSLRGANLDGKLDWNQWLGSAPKRPLDPMRFRNWYWFWDYS
                     GGMLIGQAAHVIDAIHMLMDSTFPAAVTCAAGKVDVDGAEVPETATMAVEYPENYLAV
                     FTIGYKAMRYHTFNDQMKNFHGSKARFDVWREGYALYPQSSAIEMRPTLELRKPGSFV
                     PATRSHIRNFLDCIRSRKDPNATVEMGNYTNVALCMAMQSQREGRRLRWNAASRRVES
                     "
     gene            complement(47443..49023)
                     /locus_tag="VLE22_27665"
     CDS             complement(47443..49023)
                     /locus_tag="VLE22_27665"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HSB18261.1"
                     /translation="MVILGIGGLLNDAACAIVKDGRLVAAVEQKKLARRFHPGELPDE
                     AIAICLEMAGVPTAAVDCVALVRPFSSGVETAIHLELRGAFPNAQIVLVEHHVAHAAS
                     AYYASPFDQATVLTLDRGGDFRCGARWRASGNQLTLERELYYPDSLGDLYGRVTALLG
                     FEANADEHKVQWLSATCPDDRYHELFLEILALRNGNWPRIDRSFFHGERQGHGGFSAR
                     FYQRLDLDDGAEIPPSMKPSLAAGLQQAIEETVGEMAGEGENLCFAGGLALNVLLVAA
                     LERSGRWSNVFVQPAAGNAGTALGAVYHAWHHVYGQTSRIGLGDFCLGPSYGAEEIKR
                     VLENCKLRFRYLLTTEELLETAVREIQDNRIVAWMHGRMEFGPRALGNRSILASPLDA
                     YSTENLNTYIKHREPFRKFAASVPAELADRYFEVGPNARFLATVARVKPEHKKLFDGA
                     ILGADLVRVHTVSREDNPLYWQLLHATGKATGLPVLYNTSFNLFGDPLVCTPRDAVRS
                     FYSSGIDAMFVGNFLLRK"
     gene            complement(49059..50798)
                     /locus_tag="VLE22_27670"
     CDS             complement(49059..50798)
                     /locus_tag="VLE22_27670"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HSB18262.1"
                     /translation="MPNPDTIGEDARKESGAATSPSHFIKEIVRQDVAANKNEGRVHT
                     RFPPEPNGYLHIGHAKSICLNFGLAAEFGGKCNLRFDDTNPSKEETEYVNSIIEDVHW
                     LGFDWEDRLFYASDYFDQLYQWAVQLINAGKAYVCDLTAEEIREYRGTLTEPGRESPY
                     RGRSVEENLNLFERMRAGEFPDGSRTLRAKIDMASPNLNMRDPVMYRILHAEHHRTGG
                     KWCIYPMYDYAHGQCDSIEGITHSICTLEFADHRPLYDWFIEQLGIFPSRQYEFARLN
                     LTHTLLSKRKLLMLVQQGHVSGWDDPRMPTICGIRRRGYTPEAIRAFCERIGVARTDG
                     VIEISLLEHFVRENLNRRSPRVMAVLRPLRVVIDNYPEDRVEELEAVNNPEDPGMGTR
                     RVPFSRVLHIERDDFREDPPKQYFRLAPGREVRLRYGYFVKCTGAVKDETTGEVTEIH
                     CTYDPATRGGNAPDGRKVKATIHWVSAAHAVEAEVRLYDHLFSRENPGDDSEGLDFTS
                     YLNPKSLEVLTGCKLEPSLAGAGHGSRYQFERLGYFCVDSDSAPDHLVFDRTMTLRDT
                     WAKIEKALEEKKR"
     gene            complement(50822..52318)
                     /gene="gltX"
                     /locus_tag="VLE22_27675"
     CDS             complement(50822..52318)
                     /gene="gltX"
                     /locus_tag="VLE22_27675"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSB18263.1"
                     /translation="MTTPLKPVRVRYAPSPTGDFHVGGARTALFNYLFARHHRGSFIL
                     RIEDTDQKRYNPQALAWLLNGLRYLGLDWDEGPEAGGDYGPYVQTQRIEVYRRHCGRL
                     LDAGAAYRCFCTPERLEAVSKELQRKGLNPGYDRLCRNLDPEEAARRAAAGEPHTVRF
                     KLPLDGEIAVHDVIRGDITFQNANLQDAVLMKSDGIPTYHMANVIDDHLMEISHILRG
                     DEWVNSLPLHIHLYTAFGWEPPVMAHLPLILNPTGKGKMSKREDRAPDGRKLPVFVRT
                     FEEMGYLPEAMINYLALLGWSYDDKTEIMGHDELIERFSLDRVNASPAVWNYDKLDHL
                     NGHYIRQLPVEELAERLLPFARAAGFQVEREKMLQVTPLIRERITTLGDVATVANFLF
                     MDELPPYDASELIPQKGNAAMALAALQRAGKALDAAAFTREALEVRLRDEANAMGVKA
                     GQMFQPIRVAVCGRKAAPPLFETMEVLGRATCLKRIGQAIHKLAEHAV"
     gene            complement(52403..53164)
                     /gene="rsmA"
                     /locus_tag="VLE22_27680"
     CDS             complement(52403..53164)
                     /gene="rsmA"
                     /locus_tag="VLE22_27680"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HSB18264.1"
                     /translation="MGRRLGQHFLHKDSVLHRIAEVACPDPEPLVIEIGAGTGALTRH
                     LLARAARVVAIEADPILIGRLSEKFARTANLERVHGDVLETDLSQWGPAVFAGNLPYY
                     ITSPILRKVLDLGPLLRRAVFLVQREVALRLVAIPGTRDYGFLTVLARLSSEPEYLFT
                     VPAAAFRPPPKIDSAVVRFGPLAVAEQWGIDDPSSFLEFAGQCFRQKRKTIRNNLAGL
                     FGKTLLDGTPEASQRAEQLSVGELAALYRRLRAQG"
     gene            complement(53175..53867)
                     /locus_tag="VLE22_27685"
     CDS             complement(53175..53867)
                     /locus_tag="VLE22_27685"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSB18265.1"
                     /translation="MNCHRVQRSLSAYADDVVSARERVDISGHLAGCRDCAALAQRLQ
                     LMRSALRALPVRTPPNHLTTRLTVLASRERIRRLRRGTFAGLVGELVGTARLWLENIM
                     RPLALPFAGGLVSAVVLFAVMTPVFAPPENMRVDDVPTILSTEAAFISMGPFTLSDED
                     IVVDLTVNEQGRMVDYSTPKGQRWVKDPESRKIVENALFFTLFSPATNFGQPLSGKLR
                     LTFRRTQIDVRG"
     gene            complement(53872..54558)
                     /locus_tag="VLE22_27690"
     CDS             complement(53872..54558)
                     /locus_tag="VLE22_27690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB18266.1"
                     /translation="MTESLASVETPDLLEEIAAPREAAVAVEDERLVEGLRLGAEAAY
                     ETLIQRFQQPVFNLVSRLLRDPDEACDVVQEVFLKVFRKIGLFRGDSSLKTWIYRIAV
                     NETNNHCRWNGRHRHQEVDLESCPEHGRSYRDTLPASGRSPFDYVLDRERQGLIEAAL
                     ADLNPTFRSVVVLRDVEELSYEEIAAILQVPLGTVKSRILRGRDALRRELETRLESEP
                     AFRLMPQTVE"
     gene            complement(54774..55910)
                     /gene="bshA"
                     /locus_tag="VLE22_27695"
     CDS             complement(54774..55910)
                     /gene="bshA"
                     /locus_tag="VLE22_27695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HSB18267.1"
                     /translation="MNVGITCYPTYGGSGIVATELGLELANRGHNVHFISYANPIRLD
                     PGIPRIHYHEVEVSTYPLFQFPPYCLALASRMAEVAEGYNLDLLHVHYAIPHSISAML
                     ARDMLAAKRRLPFVTTLHGTDITLVGADRSYFPITRFSIERSDGVTTVSEYLRSQTVE
                     VFGVTNEIRVIRNFVNCEMYQPEPERHEARYYSPLGDKLLIHISNFRPVKRVLDCVRI
                     LYEVRKETPAHLLMVGDGPERGPAEALARELAIEKHVTFLGKQSHVERVIPQAHVLLL
                     PSELEAFGLAALEAMACGVPPVATRVGGVPELVTDGVNGFLEPVGDVASQAARVVALL
                     TDGALHQRMSQAARETALNEFASSQVIPMYEQYYEAVCSRWVAS"
     gene            complement(55907..56785)
                     /locus_tag="VLE22_27700"
     CDS             complement(55907..56785)
                     /locus_tag="VLE22_27700"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18268.1"
                     /translation="MLTRGPDGAWDAVDALNPSVVKEGHRYLNLYSGFDGRSWRTGLA
                     LSDDGVVWTRLGQVLSPDPASWEGDYIAANGSLSRSGSQFLYWYQGGRRPAIGLARST
                     DARHWVKTGAPVLRPGPRGSWDERGVADPYVVQSAGAFYMYYLGQDRARRQRLGIARS
                     FDGVEWQKLRANPILEPGPPGTFDENGLGEPAVWASHGSYWMLYTGRDRHEQRRLGLA
                     RSPDGVRWVRADDFPVVAGGQSWNSKVVCDPSVEVDDGLIRVWFGGGNVASPDENLNG
                     QIGLAVLRPVNANLTK"
     gene            complement(56932..58098)
                     /locus_tag="VLE22_27705"
     CDS             complement(56932..58098)
                     /locus_tag="VLE22_27705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSB18269.1"
                     /translation="MVRNLAGNDNADVDAWRWVLAWTRLALAVCSVGVQFHSAASARS
                     GVTVLAAAFTLYSLAGLSRRSAGKAGGDLLSLVSDTIFFLVFAGFGSDHSLWLVSGFF
                     VYLLLSAVVTHEWWDPFIVVGASAGFFAIARPPYGGDLWRIILPGGMLACLLALRKRR
                     LWQSLSARTAEADMLRSSLATAREDERQRIAGDFHDGPLQSFVSLQMRLEVLRRLIER
                     DFHGGMSELQDIQEFIKAQVQEMRAFLRGMRPVPIEERGLSVSIRRIVAEFQKDSGID
                     VTFEGNDRTDSIAPATAMEVLQIVREALHNVRKHAGASRAAVAVTHDPTRLEIAVEDN
                     GAGFTFAGCYTLDELDQLRLGPSSIRRRVRGLGGELVLDSKPGRGSRLKIRVSV"
     gene            complement(58169..58708)
                     /locus_tag="VLE22_27710"
     CDS             complement(58169..58708)
                     /locus_tag="VLE22_27710"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB18270.1"
                     /translation="MKYLLPAIVCVGLFGQAVKENEQTDPLAPYYPTPETIVDKMLQL
                     GGLKAGEKMFDLGSGDGRIVIMAAQKFHADATGIEFDKDLWRQSTDKIKSLNLQKTAR
                     IIHGDIMLQDYSSADLITVYLLPSSNDKVRPLLEKQLKKGTRIVAHDFEFSGWTPEKV
                     EQIEDDGEGRSHTLYLYRR"
     gene            complement(58792..60726)
                     /locus_tag="VLE22_27715"
     CDS             complement(58792..60726)
                     /locus_tag="VLE22_27715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18271.1"
                     /translation="MCRNAPLLVPRRTLAIAFFALALAATCLGQQDSRRPGGLRPDWR
                     RIGSSVADLGLASGAGGPVDRVWFSEDGSSVFLRMPDGRVFQSSDQENWKLRADVETP
                     PEGDSAADFAVSMPESTGRIFRARSSPSQLYGIGRDVYRSDDEGRHWFNLTRFRRESV
                     IGEGLTDLAVSPRDPREIVAANSQGIWRSADGGMSWSGMNDTLPNLPVARLLTLPEGS
                     RGTRVVLDGAGAAEWAPGEKEAWRLVDEPSIRQKEDLLRAAGSRVSADVTAIAPGRLY
                     SYLGARDGRMWVSLDQGRTWTLTRPPGGGAVRGIYVDPEESRLALAALGPASAETSSP
                     RILRTVNGGVFWDDIGGGLPEGAANAVTADRQAGAVYAATDRGVYLTFVDLTAAGPAS
                     PWIPVGENLPAVPAMDVALDTPGNQLFVALRGYGVFSAPAPHRYRNIRVVNAADYSSR
                     PAAPGTLLSVLGARLLRAQAGYVDAPVLHASALESQIQIPFELDGQSAQLSLEGSGGR
                     YSLNFPLRKVAPAIFVDRDGTPMLIDGDGGTLLDAMNPARSNSRVQILATGLGRVRPS
                     WPTGMAAPLEDVPRVVAPVRVYIDRSPVEVTRASLAPGYVGFYLVEIRLPAIVNFGPA
                     ELYVEAGGEESNRVRIYLEP"
     gene            60902..61729
                     /locus_tag="VLE22_27720"
     CDS             60902..61729
                     /locus_tag="VLE22_27720"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HSB18272.1"
                     /translation="MWRGTKTCFVATLVCVALAGVCLAQFPLTRTTSPGAAKVVSVTG
                     QVSALRDSQPWALGVGDTVQLQQVIMTGADGYAVFQVSDGSTFEVYPNSEIVFRRTPG
                     NWKDLVDILIGRVKFTIQKFGGQPNHNRVITPTAVISVRGTVFEVEVEDDEATTLVQV
                     DEGQVEVQHAKHPGKPKILNAGEWLRVYKDQPIAKSPVDKGSVFNAAVRALSDALYTA
                     VYRNRIPTPAGSPIPGSGGGIPLPGDTGTTPPPPPPLPGDGGGTTPPPPPPPPPPGA"
     gene            61771..62346
                     /locus_tag="VLE22_27725"
     CDS             61771..62346
                     /locus_tag="VLE22_27725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18273.1"
                     /translation="MTRWLGLWCLFASLGGVPGWAQAPELPPEWEVSRDMTALAEQIR
                     RFKPILDEVKPQDWVSQGAPEAYQTQWKSITSEIEYLTRSTKELSSQPERLTVALETL
                     FRMQSLDSILSSLDEGIRRYQNPALADLLRGEANQTAAHREKLRHYVVALAATKELEY
                     KIMHEEAQRCRGMLSRQPAPKPGPSQPAEKK"
     gene            complement(62539..63552)
                     /locus_tag="VLE22_27730"
     CDS             complement(62539..63552)
                     /locus_tag="VLE22_27730"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HSB18274.1"
                     /translation="MYDLTHRDFAGVVLALLTAVCLPAQQGWHDHEGGLLKYRVQIRF
                     GEEPDTMPEGSKLGRVSAVATDSRGQVFVFHRGTKADPLLVFDAMGKYLRSWGRGMFG
                     NPHGLRVDREDNVWITDNGNHEVIKFSNDGRILLRLGTRGQAGADDKTFNRPTDIAFA
                     PNGDFYVSDGYGNSRVVKFSRDGRYLLAWGRRGTGPGEFNLPHSVAVDSKGLVYVSDR
                     ENNRIQIFDANGKFLRQWTHLGATQGLFVTALDEMWIITHRNNIENLTYDTLAGRIMR
                     IDIATGRILGSMESPGHSIDVSRSGEIFIGSLTGNVFRWRQEWLDSGAGSDEGLRPDN
                     LRR"
     gene            63607..64446
                     /locus_tag="VLE22_27735"
     CDS             63607..64446
                     /locus_tag="VLE22_27735"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HSB18275.1"
                     /translation="MVRKLMLFVPLMAIAATTPRIVVHGHRGARAVLPENTMPAFEHA
                     IEAGADLLELDLAVTKDDVLVVSHDPILNPVICRSLGGATVIRELTLAELRRWDCGSM
                     RNPEFPNQRPAPGTRVPTLDEVLSLAPRGSFQFNIETKMSPGKPQYTPSPGRFAELLL
                     AGIRKRHLEDRVMVQSFDYRTLRAMKRLAPGIRLSALFGDDARDFAAIARDAGTGMVS
                     PHQRLVTPEKVAAAHAGGLTVHAWTANKPEEWDRLISAGVDGIITDDPAELVRHLRQR
                     GLR"
     gene            complement(64449..65456)
                     /locus_tag="VLE22_27740"
     CDS             complement(64449..65456)
                     /locus_tag="VLE22_27740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB18276.1"
                     /translation="MKLALPLLLAQTLSLAQIPRIGIIDFYGVRKVSEERLLKALGAR
                     EGDPLPPSKSVVEERLEQVSGVVRARLESVCCADGKAILFVGIEEKGAPHFNFRTPPN
                     SEVRLPDQVVETYRQFLAALERAVRSGDARDDLTRGHSLLADPECRALQLKFEEFAKS
                     NLKLLRDVLRESGDDEHRSVAAYVIGYAEKKRLVVDDLQYAMQDPADAVRNNAMRALA
                     AIAVPALKDPELGIRVSPTWFIEMLNSIIWTDRNKAAFALVTLTESRDAEVLDQIRTS
                     AMDSVIEMAHWKSLGHALPAFILAGRIAGVAEEEIQRAWSDGEREAVLEKARAAGRRR
                     R"
     gene            complement(65487..65927)
                     /locus_tag="VLE22_27745"
     CDS             complement(65487..65927)
                     /locus_tag="VLE22_27745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18277.1"
                     /translation="MAFCAQCGSAVEGKFCPQCGASVGESGGTGSGAAPQAAGTGLSD
                     NVAGALCYVLGLITGILFLVLAPYNQNKTVRFHAFQSIILNCAWIVLWIALSAILPFF
                     VQAVLLPLLSLAVVILWIYLIWKTYEGKTKVVLPVIGEFAQKQA"
     gene            66418..70038
                     /gene="nifJ"
                     /locus_tag="VLE22_27750"
     CDS             66418..70038
                     /gene="nifJ"
                     /locus_tag="VLE22_27750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HSB18278.1"
                     /translation="MNRPKVTIDGNEATAYTAHQINEVIAIYPITPSSNMGEWADEWS
                     AKGKKNIWGVVPTVVEMQSEGGASGALHGALQGGSLATTFTASQGLLLMIPNMYKIAG
                     ELTAATIHVSARTLATHALSIFGDHSDVMACRATGWAMLCSNSVQEAMDFALIAQAAS
                     LESRVPFLHYFDGFRTSHEVAKVEQLQVEDLKAMMDDELIRAHRARALSPDHPVLRGS
                     AQNPDVFFQARETVNPYYIAGPTIVENVMAKFAKVVGRQYRLFDYVGAPDAERVLVVM
                     GSGAEVAHETVEYLTARGEKVGVLKVRLFRPFSIEHFIAALPKTVKNIAVLDRTKEPG
                     AVGEPLYMDVVTAISEAFARGQAPLASFPRIIGGRYGLSSKEFTPAMVKAVFDEAGKE
                     NPKNHFTVGINDDVTHTSLQLDPEFSTEDPSTVRALFYGLGSDGTVGANKNSIKIIGE
                     DTDNYAQGYFVYDSKKAGAITISHLRFGPKPIRSSYLISKASFVACHQFSFLERFDML
                     KSAQPGAVFLLNSLYGPDEVWDHLPKKVQRQIIDKKLKFYVIDAYEVAKKTGMGVRIN
                     TIMQTCFFAISGVLPREDAIAAIKKAIEKTYGKRGEAVVKKNFAAVDAALDHLHEVKV
                     PGAVTSAFDMRPAVPPQAPGFVQKTLAGIIAGEGDSLPVSAFPVDGTFPTSTAQWEKR
                     NIALEIPVWDEALCIQCGKCVLVCPHAVIRAKVYDGGALNGAPETFKAVPARWKDFKD
                     QKYTLQVAPEDCTGCGICVAVCPAKSKTEVKHKAINMAPQPPIRETERANWEYFLNLP
                     ETDRRVLSLAQVKDNQLLRPLFEFSGACAGCGETPYIGLMTRLFGDRAVVTNATGCSS
                     IYGGNLPTTPYCANAEGRGPAWSNSLFEDNAEFGMGIRLAIDNQTGYAKQLVSRLAPV
                     IGDDLAQALLGADQSSEAGILAQRERVKLLKEKLSGLKTAEARDLAAVADTLVKKCVW
                     ILGGDGWAYDIGYGGLDHVLASGANVNLLVLDTEVYSNTGGQMSKATPLGAVAKFAFG
                     GKPKPKKDLGMMAMTYGNVYVARVAFGSSDMQTVKAFLEAEAYDGPSLIIAYSHCIAH
                     GYDLVNGLEQQKAAVQSGHWPLFRYNPNLVGQGKNPLQLDSKAPTLPLDKYIYNETRY
                     TMLVHSKPEAASELLKMAQEAVQSRWKLYEHWASMPGDGQVQEAIKEAVKQTPAEESK
                     NA"
     gene            70031..71041
                     /locus_tag="VLE22_27755"
     CDS             70031..71041
                     /locus_tag="VLE22_27755"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HSB18279.1"
                     /translation="MLDLTTNYMGLALKNPLVVSPSPLCEHIGNILRMEDAGASAVVL
                     HSLFEEQITLESEELNEALSAGTESFAESLSYFPDMTRYNLGPDGYLEHIRKAKQAVD
                     IPIIASLNGVSTGGWIKYAQEMEQAGADALELNIFYLANDPALTSEQVEEMYVGLVSH
                     VKASVRIPVAVKLGPYFSAMANMGKRLDQAGADALVLFNRFYQPDFDLEELEVVPNLH
                     LSTSHELLLRLHWIATLYGQIKADMALTGGVHSAQDVLKTMMAGARVAMMTSALLRNG
                     INHLETVLADMKAWMEEHEYESVSQMQGSMSRRAVADPSAFSRANYMKVLSSYTMKTI
                     GN"
     gene            complement(71111..72286)
                     /locus_tag="VLE22_27760"
     CDS             complement(71111..72286)
                     /locus_tag="VLE22_27760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB18280.1"
                     /translation="MATVKTSRLIVAAILAILVYGMIAAMLGTLLPNLSTKFGLTPEQ
                     SGDIAFAQALGLIVASVLSGPLIDNRGKKTGLVASLILIAGALFALPNSGSPLQVMIW
                     MFVLGLGGATLVTAGSALASDINPERRATTLNLLNLFFGLGGLATPLIAANFLAGNAI
                     ALCYLVAALTVATLLFNSVTVMPPPSGERGFKFSEIGTVLGQPALYLLSFFMFLYVAC
                     EVGVWNWLPKHLIAQGVPEGQALRILSLGFALGLLIGRLVVSRILVNVAAATVTLASS
                     ALMAVTTYLALQTGDPTVAWLAVFCAGLAMAPVFPTTVAMAGDAFPKATATAMGIVLT
                     CGWVGLAVSSKVIGGIAGGDDQKLKAALLVVPVASVIMVVVNLLLRPIMARKKASAG"
     gene            complement(72398..73648)
                     /locus_tag="VLE22_27765"
     CDS             complement(72398..73648)
                     /locus_tag="VLE22_27765"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB18281.1"
                     /translation="MKRSAGAALAAGPVVLPALGANNKLNVGWVGTGSRGNYVMNMMY
                     ESVPQLVHVTAVCDTYQGHMNRAKDRVQTVEGAAPKTYVDYRELLQQPGIDIVFITTP
                     EHLHHEMALAALSAGKHIYLEKPLAHTIEEGWDIVKAAEKSGKVVQVGTQNRSNSLYI
                     MAKELVAQGLIGDIHYTRAYWYRNSLSNAPAWRYAIPPDASEQNTDWQRFLGPAAKRP
                     FDKNRYYQWRLYWDYSGGISTDLLVHQTDITNFVCGKTVPLSCMASGGVYRWVDANDD
                     REVPDTLSAIYEYPDKFHINYSCYFGNDHFGYGEDFLGNEGTIQVLNRQILHFYPESF
                     RGKPPANIAARKEIHLEIPNNDNTAVQAHVKNFVLAVLGKAKAIAPASVGQEAAIGGH
                     LATISYRNNKKVYWDEKTKNYRTS"
     gene            complement(73867..74787)
                     /locus_tag="VLE22_27770"
     CDS             complement(73867..74787)
                     /locus_tag="VLE22_27770"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSB18282.1"
                     /translation="MKVFPICVVIATLLMGGNLAAGYQPEPPDPSAPPEEVQGDFFPG
                     LHPASRSFLGIGVTEIDSDRARSLKLDEERGVEVTKVVPDSPAAKAGMKTGDIVLEYN
                     GQKVVGTEQFVRLVRETPIGRNVRLLLSRDGKTQTLTATTASRKQQQARVFAMPPGKD
                     WARLGEEMESLNELQFRMPDIPKAYMSWRTSVLGVEAESLEAQLADYFGVKEGVLVRS
                     VLKDSPAEKAGLKAGDIIIRVSETKVTTPRDVSSAIRSARSQKTFPITVVRNRKEMTL
                     TVTLEDSRSGQDHHREFRVIRNTELNESRL"
     gene            complement(74837..76006)
                     /locus_tag="VLE22_27775"
     CDS             complement(74837..76006)
                     /locus_tag="VLE22_27775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18283.1"
                     /translation="MRVHWLIPVVAAAAFGQTSSPIAQEDAYWVQTCEGTVESSPGGQ
                     LRVRADGPLIVRGAGQETIDYRLKKRVRVRDAAQAAALLKRISFNVRREGALTRLVVA
                     YPVDLSASSELELRVPRSLARCVLETRLGSIEATGLDGAVDVRAAGGRIRMDDIGGGA
                     TVRTGGGEIRLGRVGGFLRCFSGGGTIHAERVGGEALFETAGGEIYIGEARGPVHAST
                     AGGNIRVRRAAATVTAHTSGGMIDVIEADGPVIAESASGCIRVASANGVRLGSAGGTI
                     QLRGVSGALLAATASGNILAEIPSNARLEDSNLTTGRGDVTVFIPSNLAVTVQAHNEG
                     KGMSGRIVSDFPEIQVRLGRFREARPAIAEGVLNGGGPQLWISATGGTIYLRRQD"
     gene            complement(76006..76917)
                     /locus_tag="VLE22_27780"
     CDS             complement(76006..76917)
                     /locus_tag="VLE22_27780"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB18284.1"
                     /translation="MTCELARSRLSLFLYGELSFDEEEALQQHLESCEGCRHALEREK
                     VWHEAVDLHATEPPANLLAQFRQELRARLAEERRPHRTFALWEWLGEVLHAPYMRPIG
                     ALALVLAGFLAARLTAPGVSPVSQADNAGHGPTATQVRYVQPDASGGVRIVMDETRQR
                     ILSGSLEDLAIQRLLLAAASDAADPGLRVESMDILKSGADSPEVRRVLLQALRQDPVA
                     GVRLKAIEGLKSFSAEPEVRKVLADVLLTDENPGVRTQAIDLLVQHKGAGMAGLLQEA
                     LYRDSNEYVRQRCQKTLREMNASVGTF"
     gene            complement(76914..77540)
                     /locus_tag="VLE22_27785"
     CDS             complement(76914..77540)
                     /locus_tag="VLE22_27785"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSB18285.1"
                     /translation="MLTVQREAALTSDIGLTSAMDEAALIRAAQQGDQDAFERLVRAY
                     DQSVLRLAMNLLRSVEDARDVYQDAFLRVYKNLHSFRFDCSFHTWLYRIVTNLCLDHL
                     RKRKVRREESPVAHTESGTLDFMQMVPEERVDGDPQRHLMSRELGRRIGEVLRGFTPR
                     ERMVFELRHYQGMRLKAIGEVLGTSEEAAKNCLFRATQKMRQALGDFV"
     gene            complement(77610..79322)
                     /locus_tag="VLE22_27790"
     CDS             complement(77610..79322)
                     /locus_tag="VLE22_27790"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HSB18286.1"
                     /translation="MNHPTPPLPGLEDQPASLETSTFGEILSQFEESHRREGGRSEAR
                     QGRVVAVNADSVFVDIGLKMEGVLPAGDFVDAAGNAGIQPGDTVIVTIKGRDGGGYYE
                     LSKVRVERPKDWSSLERAFAEKKTIGGVVTGVVKGGLTVDVGVRAFLPASKSGIRDQA
                     DMEKFVGQEVQCRIIKLDVGDEDVVVDRRVVLEEEEARARQKVFDELQEGAEVEGAVR
                     SLTQFGAFVDIGGIDGLLHVTDMSWGRVNKPSDLLTEGEQIRVKILKIDRESRRISLG
                     VKQLSPDPWTAVGEKYSQGQRVRGIISRVAEFGAFVELEPGVEGLIHVSELSWSRKIR
                     KPSDVVKAGEAVDVVVLGVNTVDKRIALGLKQALGDPWQDIETRYPAGTVVEGPVVSL
                     AAFGAFVDLGEGVEGMIHIGDISREKRLNHPKEMLNTGQKVRAVVLEIDKAKRRLRLG
                     MKQLEPTTADEYIAEHKSGDTVSGRVLEVRSGKAKVEVGEGVHATCRIPAEPERPAES
                     TEAGSSKADLTALTAMLSAKWKQGKTPDAAVRREAVRAGQIRSFRIVALDPGNKQIEL
                     ELAG"
     gene            79375..80616
                     /locus_tag="VLE22_27795"
     CDS             79375..80616
                     /locus_tag="VLE22_27795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB18287.1"
                     /translation="MAATPWISLLRRADVFWLVLVAGLAAASPTRYPGEIPLLSALAI
                     VQVIEAKVGFFSTSRGSVAANLLKLLLGYFLIGYTGGIESSYYLVLVLPVISAAATLG
                     VIGTVAFTLLACASYLSFLLYIDWTRWILPSDQVRELGLRATFLAIVGFLAYRVAEAK
                     GEEAHRYQVTAQQLAAANRSLKEAEAAVSRSERLAALGQLSAGLAHELRNPLGTIRAS
                     AEMLRKNIPAENSVANELAGFISSEVDRTNSLVTRFLEFAKPLALRLHKADLTEVLDR
                     AVTRFQRSPAAVRVSIYKNYSPDIPPFPLDVELMERVVFNLLANAAEASPADGAVTLK
                     TRPVDGTVEISVIDRGAGIDPKHRESIFNPFFTTKPDGVGLGLAIVSKIVDQHGGKMT
                     VESELGRGSVFRVFLPLGGTA"
     gene            80626..81999
                     /locus_tag="VLE22_27800"
     CDS             80626..81999
                     /locus_tag="VLE22_27800"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB18288.1"
                     /translation="MSKHRILVVEDEDKLRRVVELQLQSAGFEVDQAASAEDALKLSD
                     RADLVLTDLRLPGMDGLELLAVLRRQNSRTPVVVMTAFGTVEGAVEAMKAGAFDFLMK
                     PFSLDHLMAVIQKALEVRALRDENISLREELGRRYTIDNIIGRSNAMQEVFATVMRVA
                     PTRATVLIAGESGVGKDLIARAIHYHSPRRDRPFVKINCSALPENLMESELFGFEKGA
                     FTGAVAAKPGKFEQADTGTAFLDEIGDVPASVQVKLLRVLQEREFERLGSNKTRHIDV
                     RIVAATNQDLRAALEQGTFREDLYYRLNVVPMNIPPLRERKEDIPFLAEHFVMKLTRD
                     AGSRVESITEAAVRKLMEYHWPGNVRELENVIERSLVLCTGTKLDAGDIKLDTTPRAV
                     APPSGQFLPEGLTLDEHEQTIIREALRRSGGNKSQAARLLGLTRNALRYRLSQMGLES
                     LDASSQG"
     gene            82045..83289
                     /gene="dgoD"
                     /locus_tag="VLE22_27805"
     CDS             82045..83289
                     /gene="dgoD"
                     /locus_tag="VLE22_27805"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HSB18289.1"
                     /translation="MNRRDFLSHTGAAGLSAQAQAIAVEGPRYAGKPALRITDIQPFL
                     VGLGGRNYCFVKVLTDQEIHGFGEAYSVGPDEATVATIRDFKHWLVGKDPRNVEYLWA
                     MMYQFTRFPGGAVINAAISGIEHALWDIAGKAAGLPVYMLLGGKCREKVRVYQSAGGA
                     TPAELAASSQRLISKYGYTALKMAPYPPGSSKLPYNAVTRAAAERAQAAREAVGPDVD
                     LGFDVHATLFEPVRAFQIAEALKPARPFFIEEPLRMENVDALAELKRKIEIPLATGEC
                     LYTKFEFREILARQAADIVQPDVCLTGGIMELKKIAAMAEAHYVVVAPHNPMGPLATM
                     VNVHFAASTPNFLILEYHPDDTSPRKDMIKDPILVKDGYLPIPDKPGWGYEMNEEAFK
                     HHPPKPWHRGFAFRSDGSPDFI"
     gene            complement(83302..84621)
                     /locus_tag="VLE22_27810"
     CDS             complement(83302..84621)
                     /locus_tag="VLE22_27810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HSB18290.1"
                     /translation="MATEANAVAKALEQKIKNRTARVGVVGLGYVGLPLAVEFAKAGF
                     AVTGVDLDKTKAERLNSGDSYVQDVPGAVLAPLVAAGKLRATTDFAAVAGLDTINICV
                     PTPLRKTKDPDMSYIVSACQEIARHMHPGMLLILESTTYPGTTDELLLPMFTRPGMRV
                     GEEFFLCFSPERVDPGNAMYQTSNIPKVVGGVTKACTGMGAMFYEQALEKVVPVSSTQ
                     VAEMVKLLENTFRMINIAMVNEMALMCDRMGINVWEVIDAAATKPFGFMPFYPGPGLG
                     GHCIPIDPFYLSWKTKQAGIEARFIELAGYINGQMPQFVVDKVQNALNDHSKPLRGSR
                     IHIMGVAYKRDVDDVRESPALDIILLLEKRGAQLTYSDPHVPVLKKDGVELCSKAADE
                     GAAEADCVLIVTDHSAFDYAKLLEKARLIVDTRNAMRAFQSEKIVRL"
     gene            complement(84634..85440)
                     /gene="dapF"
                     /locus_tag="VLE22_27815"
     CDS             complement(84634..85440)
                     /gene="dapF"
                     /locus_tag="VLE22_27815"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HSB18291.1"
                     /translation="MRIPFTKAHGARNDFLLTWTNEAPLENLPSVAEAICDRHSGIGA
                     DGWMLVTPAGPESGHHAVIRLFNADGSEAEISGNGTRCAAAFLLDSGLGAETVRILTG
                     AGLKELRVGNRDRLRYLFEMDMGRPSWKPEELRFPLPLSTGTLEVTILDVGNPQCAVF
                     VENFEFDWRKTGAEIECHPQFPRRTNVSFVRPLDRHSIEVRFYERGAGETMSSGTGST
                     GAAWAAALRGLVESPVRILTEGGPLDARWDGSVYLTGPAEIVAGGEYYLD"
     gene            complement(85437..86987)
                     /locus_tag="VLE22_27820"
     CDS             complement(85437..86987)
                     /locus_tag="VLE22_27820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSB18292.1"
                     /translation="MVLPLLAAVSAAAVLWCAREGWTLFYGDAEAHLNIARRVLDSRT
                     PAYFQIGTVWLPLPHALMIPLARDDGWWRSGLAGAIPVAVCYVLAGAFLYSAARRAFD
                     SRAAAAASLAAFALNPNLLYLQAAPMTEPVFFACLAALLYFTVRFRDTQSGGAVLGAG
                     IAALAGTLTRYEGWFLIPFVTAYFLLAAKRRRLTWALVFGAVASLGPLYWLAHNWWYW
                     GDCLEFYRGPYSAKAIYQRALVGGMAKYPGDHDWGKAWLYFHDAVMLCAGRPLAWLAM
                     AGAIAALWKRRFWALVLPALPAAFILWSMHSGSTPIFVPHLWPNTYYNTRYGLEALPL
                     LALCAGALASVLPVRARGWAALGIAAASVSPWAFAPRPDAWVCWKESQVNSEARRKWT
                     REAAVFLRENYRPGQGIFFSFGDLAGVLREAGIPLHEGFHNGNVPHWEAAAGRPDLFL
                     WEDWALTIAGDKVSDAIVRAQRHGPRYKCVKTIVVKGAPVIHIYRRAGAPAWLRPALP
                     LHGDTSGI"
     gene            complement(86995..87912)
                     /locus_tag="VLE22_27825"
     CDS             complement(86995..87912)
                     /locus_tag="VLE22_27825"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSB18293.1"
                     /translation="MSLVVVGSVAYDGIETPHGRVDRILGGACTYISLAASYFTDVKI
                     VAVVGDDFTREDEDFLSSRGIDLTGLERVPGKSFYWQGIYSADMNDRTTLRTDLNVFA
                     DFQPKLPEHYKKQPYLMLGNIQPALQRSVREQMNGLRLAGGDTMNYWIKDYREELLET
                     IREWDFLLINDSEARQLSGEHNLRRAAHAILDMGPHTLVVKRGEYGAVLFRRDGHFTA
                     PGYMLEQVFDPTGAGDCFAGGFIGYLAEQGADPSKGAIDDRHLRNAVVYGSVMGSYCC
                     EMFGVERFRTLTRAEIDGRFQEFKAFTGF"
     gene            87987..89141
                     /locus_tag="VLE22_27830"
     CDS             87987..89141
                     /locus_tag="VLE22_27830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSB18294.1"
                     /translation="MVRWVRFLSLAVIVAGYLWMHFRMGRWLAALPSVRRSPARRFGL
                     WLFVGTMGALLCLGLLLGLSPVARAVQPALWPAWVRAASFTWALLSFVSGCAVWLWRH
                     TPDFDPQRRRLLRVAGGAALAAPVAATGFGMFVERGNLRVSEVDIPLRGLPRDLDGLR
                     LAQLTDIHIGPFLSEKELARAVDMANELRPHLALVTGDFITSLGDPLDACLYQLGRLK
                     AEAGVLGCLGNHEGYAGVESYTTRQSARRGIRILRHASVRLRFGRAALNLAGVDYQSF
                     FEPYLRGAGAMVRKDSLNVLLSHNPDVFPVAARHGFDLTIAGHTHGGQVNFEIFRQSI
                     NVARFYTPYTYGLYRHGRAAAYVNRGIGTVGIPARIGAPPEIALIRLCAT"
     gene            89129..89869
                     /locus_tag="VLE22_27835"
     CDS             89129..89869
                     /locus_tag="VLE22_27835"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HSB18295.1"
                     /translation="MRYLIISDIHANWEGLEAVAADAEGRYDQILCSGDLVGYCADPN
                     SVIDWVRRNVALVVRGNHDKACSGFEDLEWFNPVARAAALWTQAALTPGNLEYLRNLP
                     KGPLQVEDFQILHGSPADEDEYLLSSFDVHQAQSYVERDLTFFGHTHMQGGFFWHRNG
                     VRRIKRTPPDVAEHRVALDPDSICLANPGSVGQPRDGDPRAAYMLYSPGERELVYRRV
                     AYDVAAAQRKIRQAGLPDQLALRLALGT"
     gene            complement(89920..>90842)
                     /locus_tag="VLE22_27840"
     CDS             complement(89920..>90842)
                     /locus_tag="VLE22_27840"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSB18296.1"
                     /translation="ALLKQYYLDQPHIPALIHVPAEFEDQEVLEELLTEKRGRKTEIH
                     TPQRGQKKAMLELAQTNAKHSFEQRFRIMKPSTKAIQEALEDALALPEAPRRIECFDI
                     SHIQGTDTVASMVVWEDGRMKKSDYRKFIIRTVDRNDDFASMREVVTRRYSRLHDEGK
                     PMPSLVLIDGGLGQLHSAAEALESLGLINQPMAAVAKREEWIYVLGQEDEPVILDRFS
                     PVLHLIQTIRDEAHRFAVTFHRSRRESKRLVSELDEAAGVGPKTVQKLLRDFGSAERV
                     RAASEDELAASVGRAAARRVRSHYADRADG"
BASE COUNT        17168 a        28168 c        28504 g        17002 t
ORIGIN      
        1 cggtacagac accaatttcg caaaagcagc gaaattggag tcagtcccgg gttttctcgc
       61 caccgctccg ccctacacag aggtggtcag atccccatga gtttgttcag ctccagcgtt
      121 gtcacgcagc ggtcgaggaa actgaactgg cccgtatctt tcacctggcg ggccgcgtcg
      181 agaaaaccgg tcatggccgc gcgatagagt gaggtcgcaa ggctgatgcg cctgactccg
      241 gccgcggcga gctcggccac tgaaaatgat ttccctttga ttcccaccat gaaattgacc
      301 ggcttcgaca cagccgcaca gacggcgcgt acggcggcaa gatcaggcag gccgggggcg
      361 aaaagcacat cggctcctac tttttcgaag gcttgaaggc ggctgatggt gtcgtccagg
      421 cttggggccg cgaagaggag attgtgcgcc cgcgcagtca ggataaaggg aaatggaagt
      481 gcatgtgccg cctccgcggc tgcagcgatt ctttcgacag ccaagcgagt atcgtaaagg
      541 ggacggtccg ggttaccggt agtatcctcg atcgtgcatc caacgagccc caccccggcc
      601 gcaagccgga tcgtttcggc cacgacctcc ggcgcgtcgc caaaaccctt ttccagatcg
      661 gcagagacgg gcaaatccgt ggcatcgaca atcatgcgcg cgtgcgccag cgcctcatcc
      721 cgcgtcagcc cgccgtctct tcgaccaagg gcagaggctg aggcagcgct cgatgtggcc
      781 aatgcctgga agccaagccc ggcgagaatg cgagcggaac cgacatccca tggattcgga
      841 atcacaaagg cgccagggcc gtcgtgaagc gcgcggaagc gagctgcccg ttcgttctgg
      901 gtgacaccca taggatttac ctcgcgtgtt cagatctcta cccatgctac tcttcccagg
      961 gaggggaacc gccccaccta agctcccgac ttgccgagct gtacggccag catggagatg
     1021 gagccgcctt cgatgccttc gacgggaaac gtgatggagt cgcccggccg ccgggcgccg
     1081 attacgtaca gcagaggcag gtagtgatcc ggcgtaggaa tggcgagtcc cgcatcccgg
     1141 cccatcgttt catagtcaat gagcgacttg tcgtctccgg caagcagcag ctcctttgcc
     1201 acgccctcga agcgcatcgc ccactcatat ggctccgccg gccgtcccca ggcgtaggcg
     1261 cccaagttgt gcacaacgtt cccgctgcca gcgatcagga tgccttcctc ccgaaggtcc
     1321 gcgaggcgct ggccgatctc atagtgaaag gacgccgggc gcatccgatc gatgctgagc
     1381 tgaaccacag gcacgtccgc ttgcggatag acgtgccgga gtattgacca agtgccgtga
     1441 tcgagtcccc agtcgtcgtc aagcgccaca ggcaacggag caagcagttc ctggacgcgg
     1501 cgggcaagct gcgggtcgcc cggcgccgga tactgcactt cgtacaactc ctgcgggaag
     1561 cccccgaaat cgtggatggt cctcggagcc gtaccgatgg tgacgcgggt ccccggcaca
     1621 taccagtgag cggagatcgc caggatggct ttgggggtgg gcgtctgtcc gccgatgcgc
     1681 cgccatcctt gggtgtagcc attgtccatc agggcgttca tggggttgcc atgcccgaag
     1741 aagatggcgg gcaatgtttc ggccatgctg ggaggctcct ccatcaggtc ctcttccagg
     1801 atctctcaga ttaaggagcg gcgggatttc gcggcgcgcc cttggataga atcggtgcag
     1861 gattatggta acggcaatcg ttctaatcaa cacagcgcaa ggacgcacgc cagaggtcgc
     1921 gcaggcgctg atcgacctgc cgggaatcac cgaggcctat tccgtcgccg ggtcgtacga
     1981 cctcgtcgcc atcgcgcgtg tgcgcgacca tgaagggctg gccgacctgg tggcggggcg
     2041 cgtccagaag ataccgggca tcgaagccac gaacaccctg atcgctttcc gggcgtacag
     2101 ccagcgcgac ctggaagcta tgtgggatat cgggaacgcg gggccaataa gttaagccgt
     2161 tccgagatcc tccggaagcg cctccgcgcg cgtcgccggc aaagcgccag cggtacaggc
     2221 cgcctgtagg gcgacgttca ccgaaagtca gagccatggc caaggcagtc agccgaacat
     2281 tgccgcgggg agtgattgcg ctgggactcg tcagcatgtt catggacgtc tcctcggaga
     2341 tgatccacag cctgctgcct gtcttcctgg tgggtgtact tggcttgaac gccttggcag
     2401 tagggctcat cgagggcatc gcggagtcca cggcggcgat cacgaagatc ttcgccggag
     2461 tggctagcga ctggatcggc aagcgtaagc ccctggtgct tttgggatat ggcctggccg
     2521 cgcttaccaa gcccctgttt ccgctagcca ccggggcggg cgccgtcctc accgcacggt
     2581 ttctcgatcg catcgggaaa ggaattcgcg gcgcgccgcg tgacgccctg gtggccgatc
     2641 tgacgccgaa gtcgatgcac ggcgctgcct acggcgtccg ccagtccatg gacactgccg
     2701 gcgcgtttgc cggacctgcg ctggcgatgg tgttgatggc gctctccggc gacaactttc
     2761 ggctcgtctt ttggatcgcc gtgttgccgg cggtgatctc cgtggcgtgt gtcgttttcg
     2821 gcgtgcagga gccggagacg cacgccgttg tggggaatag gcgctttccg ttgcagcgcc
     2881 gggaaattcg ccgcctcagt ctcaagttct ggctcgtcgt catgttcgcc tccatgctca
     2941 ccctggcgcg cttcagcgaa gcgttccttc tgctgcgtgc cgcgaacgtg ggccttgcgg
     3001 tcacttatgt tccggccgtc ctgatcgtca tgaatgtgat ctattcggcg agcgcgtacc
     3061 cgttcggcca cctctccgac cggctcaacc gcggcagcct cctggcagtc gggatcggat
     3121 ttctgattgc ggcggatctg gtactcgccg cggcgaattc gacgtgggca gtgctcgccg
     3181 gttccatgct ttggggactg cacatgggag cgacccaggg cctgttgacc acgatcgtgg
     3241 ctgcgaccgc cccggcagac ctgcgcgcca cggccttcgg gctgttcaat ctcgtcacgg
     3301 gcgcagcgct tctgctggcg agcggcctcg caggatttct ttgggcaaac tatggacccg
     3361 ccgtgacgtt tctcgcgggc gccgtctttt cgacggtcgc gctggccggc ctgctggcgc
     3421 gacgtcccag gagcgcgccg taagcgtgga cgccaggctt tgtctctgct acacgatttc
     3481 aaatcaatgt gtggaatcat ttgatgagcg gcatgaacgt tcgcatccgc atggtgaccg
     3541 tattcctcct ttgtgtcagt tctccccttg ccggacaggg cgatcccgcg gtgaaccggc
     3601 cgaagatcgg cctggtcctg gggggaggcg gggcgcgggg gctcgcgcac atcggtgtac
     3661 tcgagtggct ggaagaacat cgaatcccgg tggactacat cgcagggacc agcatgggcg
     3721 ggatcatcgg cgggctctat gcgatgggga tgaaaccggc tgagctgcga gatctggtca
     3781 acgggatcga ttgggaggag acgctcaggg ggacaccatc ctacgagcag ttcctgctgc
     3841 ggcgaaagca ggatcgccgc gattatccgg gcctgcagct tggatggaac aagggactcc
     3901 gattcggggc gggcttgaac ggcggtcact tcgtgagttt gatactcggc cgagccactc
     3961 taccgtactg gcaggtgcgc gatttcgacg aattgccgat tccattccgg tgcgtcgcgg
     4021 cagatctggt cagtgcaaac gcggttgttc tacgcgacgg tccggtcgct gaagccctcc
     4081 gtgcgaccat ggccatcccc ggaatattca cgcccgttca tcgtggcagc gcgctcctca
     4141 tcgacggagg agtccttaac aatgtgccga cggatgtggc gaaggacatg ggagccgacg
     4201 tgatcattgc cgtgaacgcc ggcactcctc ttcgcacccg caaggatctg caatctctga
     4261 tcggcatcct cgaccaaacc accaaccttg ccgcagaaca gaacgtgcga aagaatctgc
     4321 ttctcgcgga tgttgttctc acgccggatc tcggcaagta cacggcagtg gacttcgacg
     4381 ccagggaagc ccttgcccag gcgggctacg cagcggccgc cgccaaggag ccggcactgc
     4441 gacggtttga gatcgggcgt ggagagtggg agagatactc cgccagcgtg agcggccgtg
     4501 ttcgacggga ggtcccgacc ccgcagtcac tggttgttga ggcgccaacg gccggagcgg
     4561 caaatgcgat tcgtgagcgc ctggggcggc atcggggaag aacgctgaac gtggatgcgc
     4621 tcgaggacga cctgacgacg atgctcggtt ggggcaggta cgccagtctg agttacgaac
     4681 tccgggagtc aagcgacccg tcccgcggtc cggagctggt ggtccgtgcc gagccgttcg
     4741 tgaatggaac agcggttatc cgagtcgggc tcagcctgca aggggcgcac acgcagcagg
     4801 tcgaggtcaa cctccgggca cgagacaccg tgtacgacgt tggcgggtac gggtcagaga
     4861 tgcgcttgga cgcaactctt ggcacgacac tgtcgtccgg agtggagtac taccgtccgc
     4921 tcggcaagag ccggtttttt gttgcgccgc gggcatatgc cagcagagcg acaacgaacg
     4981 tcgctgaggc gaaggatgtc ctggcaaatt accggcgttc ctccgcggga gccgggatcg
     5041 atctgggata cggtgttctc tcccggtccg atgaagtgcg cttcggctat gaagtccgtt
     5101 atgacgacac ttccatcgtc atcggcaatc cagcgttgcc cgtctacggc ggtaccgcga
     5161 atgtcctctc gttgcgctgg tttcatgacg ggcaggacag cgcgacaatt ccacgcagag
     5221 ggattcagtc cacgctccgc gggagctggt ttctgaacag cgcgacgtcg gcgcgggact
     5281 tcccacaggc ggagttcacg gcgtccgcgt tccaccctgt cgacgcgaaa ggttccgtct
     5341 tcgccacggc cgctggtgga acgacattca atcgcattgc tcccttggac caggaatttg
     5401 cccttggcgg accccggcag ctcagctcct atgggttcta cgaactgcgc ggatccgact
     5461 atgcgctggc cagcgcaggt tacctccgcc agattagcgc gaacagcctg gcaaagttgt
     5521 atgtcttggg cttcctccaa gccggccgcg tgttccgggc tcgatcagcc gaccggaccg
     5581 gtatggacgt gacggccgga gtggtcgcgg acacctttat cggaccgatt ttgctcgggg
     5641 tggccgtggg tgacgccggc caccgcaagg cattcatttc catcgggcgg acgttctagc
     5701 ccgtatttct caccagcgat ctactgcggc gcgcgatagg accgaaactg cggcgttgcg
     5761 ctccgtcgga gtcctcgacg tactgtcaag tacgcctgcg ggctcctccc gtcgcgtgcc
     5821 ttgcatttcc ggccctctcc cgcgcctcgt ccacgaccgt ggtgagaaat acgggctagg
     5881 gattggggcc ggctccatgc tcgggcgggg ccggcagatg ggcatccgag cgcaactacg
     5941 aagggaaaca cctccggaac gccaacagaa aacgaggaga gcccttgatg cggatctttc
     6001 gccgcacgat cgcccagagc gggttcgcat cccgggccag cagccgaacc cacgtgaggc
     6061 tgtccgccat cacgtgcaag ttgcatttac ctgagagccc ctcggtgaca cggagccgct
     6121 ggccgctgat cacgaccgtc gctttccgtt cttcccggcc ggtaaaggtg aagtgatagg
     6181 tagcgttcag acccttcgac tttccgcgtt ggaaaaccag cggcaggcca ctcaggaacg
     6241 cgttgaccga ctcgactcgt aagccgttgc cgacctgctt ggttctcttg tgcggaaacc
     6301 gccgcttcac gtacgcctcg gcatccgatc ccggcacgac atgcacggtt tcttccttgt
     6361 cctgcaaagg cttcacaact tcgcggagaa acctgggcct gtccgtgagg aacggagcaa
     6421 tcacgtcttc tcccgccggg cagaccgaga ggcagtaggc ggctttgtag ttcgcgccaa
     6481 aagacaggct ctgccacatt gatgcacttt cggaaccgct gaccttttgc cggtactccc
     6541 gagcgcttcc gctttcggcg attgtttcca cccagtccgt aaagccgccg agaaattccc
     6601 ggtaattgtg ggtgttgcac gccgagaaat cgaagttacc gtcggcggag atcgctcctg
     6661 tcggacaggc tgccacgcac agcttgcatt ccaggcacgg gttgtaatcg atcggattgt
     6721 tgtattcggt gacccgggca tcgacgagca cggtcgccag cagaatgaag cttccaaact
     6781 tcggatggat tacgttgcga tggattccca tccgccccag cccggccgcc accgcgaccg
     6841 gcttatgcga aacgacccag attttgccgc cgggaaagcg actcgtctcc atgggaaagc
     6901 ccgcggccgg gttcaatgcg cggatgccct tctcctccag ggttgagaca atccgggccg
     6961 ccgcgtggtt aatcttgtcc gtgacccgat gaaactcgac gttggccacc gatcgggcga
     7021 cgctccgaat gggctcgcgg ttcatccggc agaccaggct cacaagagtc tgagttccag
     7081 gaaatagcgt caggatgtcc tgccgctgat cagccaactc ggggtgatcg atccggacga
     7141 agccgacgtc atcggctccc gcctggaaac agagctgtcg caaccattga tagtccagac
     7201 cggcgttcga cgcggccgcg gcagactcct gcccgcgctc gcgaagccat tgaactgttg
     7261 ggtgttcgtt aagctcaacc attgtgcacg ctcctttaga tagatgtaga tacatctatt
     7321 ttgccaaaaa agaagatcac atcgccgacg ccgcctgggc ggctctgacc gtgatcgtcc
     7381 tcaggttctc ccaatcgcgg tcgcccaagc ccttccggag ccgttcctgg gcctgctgcc
     7441 aaaggggctc cgcctgtttc agttttcttc gtccgggcga cgtcaactgc agggagtgtt
     7501 cgcgcttgtc cgagccaggg acgctcgtaa tccaaccttt ctgaatcagg ggacgtaacg
     7561 ttcgggtgag agtcgtacta tcgatgccga gcagttccgc cagccggcct tgcgtgactg
     7621 gcgtgattag atggagcgct tgcaggagag tgaactgggt ggtcttgagt cccgccgcgc
     7681 gaagctcttc gtcgtagact tgcgtcacca ctcgagacgc gcgacgcaag ttcgcacagg
     7741 cgcaaggcaa cgcaatcaag ggctttacct cgctcggcat ctcgcctcaa cctatctatg
     7801 tatatacatc taatttccgc gtttgtcaag ggcatgccgg ttagttcacc gctggaacgg
     7861 gctcttacgg ctttcgacca cgctccgttc gcttctcgag ccgctccagc gcgggctcaa
     7921 gccgctgttt cagtttgagc accggctcaa acagatcgcc cgcccgctcg aaccgagtca
     7981 gcgcattccg gatatggaac agccccggct ccaacccctc ccttacttcg ctccagtcga
     8041 ggggcgtggc gacaggcgca cccggatgcg cccgaagcac gtaaggcgcc gaaatcgttt
     8101 tgccgcgagc gatctgcaaa tagtcgaaat acaccttgcc cttttcacgc ttggctaccg
     8161 cacgcggcaa cgtgaacagg tccggacgct cgcgctccgc cagccgcgcc aggatctccg
     8221 caaaagcccg aacgtgatcg tacgagtaga ccggatccag cggcacgtag atgtgcatgc
     8281 cctttccgcc ggtggtcttc gggcacccgg tgaggccgga ctcttccagg agcctccgga
     8341 tcaactgcgc agcctccgtc acacgcgagt agccgcactc ctgcgggtcc agatcgatca
     8401 gcaggaagtc ggggttgtcc agcgagccca cccggctcat ccacggattc tggtcgatgc
     8461 agcccaggtt cgccagccac accagcgcgg cgcgcgagtc accgatcacc tgaagacgca
     8521 ttttcccgtc ctccgccagt acacgctcct tccttagcca ctccgggaac ccgctgccgg
     8581 atgcatcctt ctggaagaac ccttccttcg caatcccgtc cggatagcgc cgaagcgaca
     8641 gcggccggtc cttccaatag gggatcagta gctcagcgac ggcgttgtag tactccaaca
     8701 cctctcgctt cgtgatgccc tcaccgggaa aaaatacctt gttcagattc cggatctgca
     8761 agcggtgacc ttccacctcg atcgtggctt ccgccttgtc ccctggaagc agcggagcgg
     8821 attttccggc cggctcctcg gcggccggct cactcctggt gcattcgctc ggatcgatgt
     8881 cgtcccgaag tccgacgaac accggcgcgc gcacgcgccc ttcgttggtc cactcaagga
     8941 accgcacggt gcataccagt tcgggccgca cccactgcac ttcttgcggg agcgagacct
     9001 tctcgcgaaa cggcgaacgc tcggtgcgca gctcatccag cctctcccgg atctgcttca
     9061 tgagcttccg gtcaaaaccc gtacccacgc tgcccacgaa ttccagttgc ccaccctcgt
     9121 acacgcccag tactagagcg ccgaagtaat cgcgttcgcc gtgcgtgtag ccgcagagga
     9181 caaactcctg ctcacgcgta gctttcactt tcacccaatc cgcggagcgg cgctcctgat
     9241 aggggctcgc ggtgcgtttc gccacaatgc cttccagtcc ctgcgcccgc gcggcttcca
     9301 gcagttgccg gggatcgaca tcaaaagttt ctgagagccg caccgccgtc cccgggcgca
     9361 ccacctcctc caggagtttc cggcgctctt ttaggggtac cccgcgcaaa tcgaacccgt
     9421 tccagtacag gaggtcgaac gcgaagaaaa cgaccggacg cgaccgcgcc agttgagcca
     9481 ccgcgttcgc gtcacgcgcc atgatccgcg gctgaatgcg ttcgaaactc ggacggccgt
     9541 tctcatccac cgccgcaatc tcgccgtcca ggatcgccga ttccgccgcc acatgatgcg
     9601 gcaacacgcc cagctccggg tattggcgtt caatggatgt gcccttccgc ccggcgatcc
     9661 gcagatggcc ctcgttaaga aagcacaaag cgcggacacc atcccacttc acttcgtaca
     9721 gccactctcc gctccccggt ggctccgcgg cggacaccgc caacatcggc tggatggagt
     9781 ggggcattgg cgaagacagt gcaccgtcgg gagcgtcccg cgccttcttc tccggctccc
     9841 tcgccgggag ctcttgcgcg atctcttcct gcgtccgccc ggttgccacg ctgtaggcgt
     9901 actgctcgac gtcccatcca ggtctcgcct cggcgtcctt cttcttgatc agcagccagt
     9961 cgttgccctt gcctttcgcc cctttcatgt gcgccagaac gaactcgccg cgcaactttt
    10021 ccccgtgcag gatgaatttg aagtcacctt tctccagcat ctcctccggg cttttcgact
    10081 cggcgggctc ccaccagccg cgatcccaca gcatcacgct gcccgcgccg tagtttcctt
    10141 tcgggattgt gccctcgaag accgcatact gcaagggatg gtcctccacc atcaccgcca
    10201 gcctcttaat cgcgggatcc agtgacggcc cctgcggaac cgcccaagac ttcagcgttc
    10261 ctcctaactg gaggcgcaag tcataatgca gccggcgagc atgatgtctt tggacacagt
    10321 acacgcgtcc ttcgggtgcg gactccggat ccggcttcgg ctccggcgtc ttctcgaagc
    10381 tccgttttct cgagtactcg tccagcggtt ttggcattgg ggtgcgcaaa cccttgaaat
    10441 ccctgcctct tctatgttaa gcagtgtata agcccgtaca tttttgtgcg gacatgtttc
    10501 tgggaagggg ttgccaccat ggcttccagc gtttggaagg gccacatcac attcggcctt
    10561 atctctctgc ccgtgcggct caccgccgcg gctcgtagcg agaccgtcag cttcaaccag
    10621 cttcacaagc aggacaactc ccgcgtgaag caagttctat tttgcatcgc cgaagacaag
    10681 cctgtccccc gcaatgagct cgtcaagggc tacgagtacg aagacgggaa gtacgtcgtc
    10741 atcgacgaag aggacatcaa gaagatccag ccgaagacag ccaaggtcat ggagatcctc
    10801 gaattcgttg acgcacgcga cgtcgatgcg gtctacctcg aaagctctta ctacttgcac
    10861 cccgaagagg ccggcgaaaa ggcgtacacg gtgctcttcg aggccctcaa gcgcacgggc
    10921 cgggccggcg tggcaaaggt cacgatgcac aaccgtgagc acgtcgtgat cctgcgtccg
    10981 ggcaagaggg gcctcatgct ccacacgatg tactaccagg acgaggttcg cgcgagcgat
    11041 gagttccgca cggacggctc catcgtgaaa gatcccgaag tggccatggc ccagcagttg
    11101 attgaggccc tggtggtgcc cttcgatccc agcaagttca gcgatggcta ccgcgagacg
    11161 ctcagggcga tgatcgacgc caagatcagc ggcgaggagg tcgtcaaggc gcccccggcc
    11221 caggaacttg ctcctgtcat cgacatcatg gaggcgctca agaattcgct tgtggcgctt
    11281 cgcaaacctc ccgctgtgga gaccgttacg gaaatcaccg ccaagcccaa gcgccgccgc
    11341 gcggcgggct aggagagccg ccgaaagaag tcccgtgtgc aaccgatcct gcggtcgttc
    11401 aatccgagcc gtgttcggac aggaaggttc cccgctcgag ttcttcgaag gcctgcctga
    11461 gctgctcttg cgtgttcatc acgatcgggc cgtaccacgc aactggttcg gctatgggct
    11521 ttccggagac caagaggaac cggatcccgt cgtcgccggc ccgtaccgtg agttgatcac
    11581 cattgtcaaa gagtacgagc gagcggttgt ccgcctccgt gggaggcgcg gtgtccgccc
    11641 atccgacccc ttcagtcggc accgcaagag ggccagacgc attgcagaac tttccagccc
    11701 ctgagaaaac gtacgcgaag acgttgcgag tcgtctcaac cggcagtgtc ttcgtccggc
    11761 ccggaggcac ggaaacatcg aggtacagcg gatcggcggc gaccccgtcg actggccctt
    11821 tggtccccca aaagcttccg caaacgatcc gcacacgcgt gccgtcgtcg tctttgacct
    11881 ccggaatgtc ggcggctttc acctcctgat agcgcggcgc cgtcattttg agagccgccg
    11941 gaaggttggc ccacaactgg aacccgtgca tccttcccac ccgatcaccc tttggcatct
    12001 cctggtggat gatgccgcgc ccggccgtca tccactgcac gtcgccagcg ccgatgacgc
    12061 cctggttccc catgctgtcg ccgtgctcca cggttcccgc taggacgtac gtgatcgtct
    12121 caatcccgcg gtgggggtgc cacgggaagc ccgccagata atcctccgga acatcattac
    12181 ggaagtcgtc gaggaggaga aacgggtcga agtcggacgt gttgccgaag ccgaacgcgc
    12241 ggcgcaggtg aacgcccgcg ccttcaatcg ccggcttggc cttggaaagc cgtcttattg
    12301 gtcgaaggga catggagcct cccgtatcga gagtataccc tgcggggcgg acgcaaagat
    12361 tctcgtctcg cgactggccc aagaattgct cactgccttc tgagtggagg cgaacggaat
    12421 aacgtcgcct ctggattcgc aagcaatgcc gtgaactgca ttggcaattc ttacacgaat
    12481 tttcggctat ccatgccgtg aactacgtct ctagtgtgag taactaagga ggtaacaaca
    12541 atgacacgga acgcacaatc cgcggttctg atcctcgggt gctgtactgc cctgttgctt
    12601 gccgggcaag gcaaaggtgg aggccaagga agacggatgg gcggcccgcc gtccggcgcc
    12661 ccgcagtccc gaggtcaggg acagcaggcc catggggcca acccggcgaa atccggcggg
    12721 cagggcgcgc atggctccgg ccagagcgca gtggaacatc tgcagcgcca ccctgagcaa
    12781 gcggcgcgcg tgcagcaaat gttgccgccg ggcacggaca tgaacagtgc agcggacggc
    12841 ttcaagagct tcgggcggtt tgtatccgcc gtgcaggttt cgaaaaacct gaacattccg
    12901 ttcgaccagt tgaaaatgaa gacgaccggg cctgaagcga agtcgctggg cgacgcgatc
    12961 cacgagctcc ggccggatct ttcccgggac aaggtgaaag atgcgatcaa ggccgccagg
    13021 caggaggaaa gacagatccg gagggagagc cggagggagg tgataggaag aaacccaacg
    13081 aaatcctagc cgcgcttatg gcagcatggg cgacggaaaa tcgaccgggg gtcgctgcgc
    13141 tcaggcggac cgccccactt gaccggcgtc aattgaccac gagtttcgtg tcggttccgc
    13201 cgaggcaaat ttcctggatg cggaacttgc cgtctccatt ggagtaccgg ataaatgtcg
    13261 tcttaaattt gctcccagcc gattccgctt tcacctgcgc ttccgcggac tctttgccgc
    13321 ggtggaaaac taccttccct tccgtcactt ccagcttgta ggcgccggct gccaactcct
    13381 tgtcgcccag atacgacgcc tgatacaggt tgaccttgaa ggtctccgcg ctcgcgatgg
    13441 atagtgcggc aaccgccaaa gtcaacagta gttttttcat attcgatctc gctccttgtg
    13501 gcttttccta tctctgttag atacgaggtg ggagcggaaa agtgctggca ttctgggttt
    13561 ccgcggaaat gttacatttg tttgcgaatt tgctcgagaa ccgcggcttg cacgtccgga
    13621 tcgtcgccga atccgccgaa ccgttggatt ccgggttcgc gccggaacca tgtccactgg
    13681 cgcttggcgt aacggcgggt gttgcgctgc gcctgcagaa ccgcctctgg aagacttagt
    13741 tctccgcgaa tgtgctgaag ggcctgtttg tagccgtggg attccagcgg tttcacctcg
    13801 ggcgagaagc caaggtcgag gatgcggttg acttcgtcga caaggcccgt ctcgaacata
    13861 cgctcacagc ggcgatccag gcgttcgtag agggcctggc gcggcggatc cagggcgatc
    13921 ttgagcacgt ggaagcccgt cagagggtcg cgcccggcgg cgaacgcatc ggtgagaggg
    13981 cggcgcaaca acagacagac ctcgacggcg cgaacgagct tgtgggtgtc ccgtgcgtgg
    14041 atccggcggg cggcttcggg gtcgaaccgg cgcaggaggc ggtggaggcc gccgggtcgg
    14101 cgcgcctcac gagcttccag acgggcgcgc acggtatcgt cgcgagccgg tcccgggaat
    14161 aagccgtcga gcagggcgcg gacatagaag ccagtgccgc cggcgacgac cggcagccgt
    14221 ttgcgcgcgg cgatctccga aatcacccgg cgggcacgcc gggcgtactc acctgccgag
    14281 aagtgttctc cgggatccac gatgtcgatc agatggtgcg ggatgccgcg gcgttcgaac
    14341 tccggcagtt tcgctgtccc gatatcgaag taacggtaga tctggaggga gtcgcagttg
    14401 acgacctcgc cgcagaagtc ttccgcaatg cgaagagcca agtcactctt gccagatccg
    14461 gtcgggccgg cgactacaac gagcggcggg tcagcggttg ccacagtgaa tcagtgtagt
    14521 gctagtcgat cccaagttcg aagaggatct cctgttcggc ggggcggacg tagcggccgc
    14581 gctggggcac caggcgaagc gggaccgaga gagcacccac cgcgtgctct acgctgcttt
    14641 ccggtgcgtt ggcatacggg tcgaagggat agaccggcca tgcaagccgg gctgcggggt
    14701 ccaccttcag cgtccagccg tggtggcgga tccagccgcc aatctctgcc tgggagagct
    14761 cgatcctttc cgcaccgagc ctgatcgact tgccggcccc ggtttccagt gtctcgccag
    14821 ctttcaaccg aagctgcaac gtcagccgcg ggtcctccgc gggcgtccct ttcccggaaa
    14881 tcacgaaccg cagaatgaga cctttcctgg taggcggcgg cacgtagagg tcggcaaaaa
    14941 aggtgttgta ggcgagcgac agacggtcgc gcgcctcttc catccgcagg cgactgctca
    15001 caggcatgtg gaagaaccgg ccgaggagct tctcgaagaa ggtcgccaac tccggctgat
    15061 tcttggagcc cgcgcccgtg atgatcaacc cgagtttgtc gtgaaagacg ctgacgtgtc
    15121 cctgccggtc gagatagaac tggttgttga cggcttgcgt gctgatgatt cccgaaagac
    15181 acaccaccca cggtcccgtc ttgcgaatgc cggcgggaac actgagccgg cgcgagtaat
    15241 ttgacaggtc ctgtgggatc ggcgccgtag ttccgcggtg gtagtacagc gcgttttggg
    15301 cgagccggcc caaatcttca tagcccacct cgccttcgcg gaagaagctt gtaaggaact
    15361 ccgcgtaacg gcgcccgtcg gggaagcgcg agaagccgaa gtggcctttg gacgcagact
    15421 cgtcgttccc gcccggcgaa ggggcgccat cgagccacgt ggaaaaccca ggcacgttcc
    15481 agccgggaac gatggtgtac ctattccggt cgtcgagcac gtcgaccggc gctccgtccg
    15541 gataggtgaa gtacttgtgg aagtcgagac cgcgccgcag cgcctcgagc gccgctgggt
    15601 cgtcggagtg ctcggcgtag agcgctacgc cggtgtaggt ggtgtagtcg taaccgggcg
    15661 tcggcagggt tgcttcatgc tctccccaaa agccgtcggg tgactgttcc aatgccgcga
    15721 agcggtgcat gacggcggct ccgatcttct cccactcctt gttctgaaac atcttccccg
    15781 ccagatggat ggtggaagcc cagagcgaaa ggtggttggg cgaggtccca ttgaacggcg
    15841 aattgtagcc cgggaagtcg atgcgggccg ccgaatccgc ggcaagttcc gcgagattgc
    15901 gttcgagttc ccgcctccag cgcttccggc gctcctcgcc gagctcacgt tccagcagcc
    15961 tgtacgattc gagccacatg taggcatcgc ggtcgctgtt gaggcgtttc tcgaacgttc
    16021 cgagctcgct gtcggacgcc atccggtcgc cgatgcggat cgccagcgcc agcatcttag
    16081 ggtcgtgata ctggcggttg gcggggtcct gtttggcata gaggacggcg gcggccagga
    16141 tgacgtgcgg gaagaggcgc cagccgtcgc cgcggcgttc cagtttctcg aagtccagcg
    16201 attgctcttc tgagagttgt ttttcgacgt gagcgacgcc ggcgtccaaa agacggaaat
    16261 agcggtccgg caattcggcg ccggtggacc gcgttggcgc cgcggcgaat gccgggtgaa
    16321 tcataagcag aacgagtgcg atccggcaac ggatggcagc tttcgtcatc agacaaacct
    16381 ccggagggcg ggaattggac cagaatggcc gctcggtgac ggtcgcggtt cagaattaga
    16441 gcacgattcg ttcatcgcct tcacgccggg tgacgcgctc ccgatcgagg tactcgagca
    16501 gcgggattgc gtacttgcgc gagatacctg tccattcctt gaataccggc accgtgaagc
    16561 gctgaccgcg ccgggcggca agctgttccc gcaacgagcg cagcgcgtcc gcgtggaaaa
    16621 cgagctcgtc accgacccgc acaagcctct tttgccgcaa caggatctgg agcagagagc
    16681 gcgcccgtcc cgcttcgacg cctgacttgg ccagtacctc gttcaccgga ggaaccgtca
    16741 gtcctgcttc agcgaacagc gactcaatcg ctttgacggc ctgttcctcg tcctgcttca
    16801 acacaagacg gtgcgatttc aggcgtacga cttcgccctc cgcgaccacc agcggagagg
    16861 ccgccaacag cgcttccaga aggaacagag gagcgtccgg cagttcccgg ctgcgcagct
    16921 cgtgccgggg gaggccagga agcagtggat tcgcgcggtg atgttccgag agcacggact
    16981 ggatgcgggc ggtggcctgc tggaaccatt cgctgtctac gagccagggg tcgggctgat
    17041 cgaggcggat aaagttggcg gagcggactg ccgcatcaac ctcttcggcg agcagaccgg
    17101 tgcgcgcgat caattcggcc aggcccacgc cgaatcgcga ctcgcgtacg agcaaggcga
    17161 tccgttccgc gggcgtgccc tccgcgagga ctcgcaatct cgccgcggca tcctcgtgcc
    17221 ggcggtaggg ccgagcaccg gtgtcgacga ccacgccgcc gccgatggtg acgaccggcg
    17281 agaacatccg cacgatgaaa cggtcgccgg gcaggagcag caccgggtcg cggagaacga
    17341 tgcgggcgaa cgtccggcct ccgggctgaa gggccgtcga accgtccagc aggcggaact
    17401 cggcctcgat ttcagccgtc ccggcatgga agtgaaccgg cgcacggtgt ttcaaggcct
    17461 tggccgaggg caacagctcg aaccggcagt cgatcacggc gccgggctga aaccggctgg
    17521 gttcggagag aaccatccca cgcgttactt cagaaggctc gacgccggac aggttgagtg
    17581 cagtgcgctg tccggcgatg gcgcgttcga cctggccgcc gtgaacctga acgccgcgaa
    17641 ctcgacagcg acgccctatg ggatagagtt cgacctcctg ctcgcgggca accgaacccg
    17701 agatcagcgt gccggtcaca acggccccga agccacgcat cgagaacgcg cggtcgaccg
    17761 gcaggcggaa gtggcggccg gcatccttcg gcggagcggc ggcagcgatt tcggcaaggc
    17821 gcgcgcgaag ttgatcgagg ctgtcaccgg tcgtcgaact caccgccacg atgggcatgc
    17881 cctccaggaa ggagccggcg acaaattcct cgacttcaag gtgaacgatg tcgaggatgt
    17941 cgcgatccac gaggtcggac ttggtgagga cgacgacgcc gcgcgggatg ccgaggagac
    18001 ggcagatgtc aaagtgctcg cgggtctggg gtttgatcga ctcgtcggca gcgatcacga
    18061 acagtacgag gtcgattccg ccgatgccgg ccagcatgtt cttgacgaaa cgttcgtggc
    18121 cggggacatc gacgaagccg atgtgaaggt ccggcgggag atccatgtgt gcgaacccga
    18181 gatcgatcga gatgccgcgc cgtttctcct cgtcgaggcg atcggtgtcg atccccgtca
    18241 gcgcgcggac cagggctgtc ttgccgtgat cgatgtgccc cgcggtgccg acgataatat
    18301 tcctcatggg gttgcttctc atcctactcg cgctggccgc cttcgcgcag gagcaggaga
    18361 cgccggtgtt ccgctccgac gtgacgatgg tgcgggtgga cgcgcaggtg gtcgacggca
    18421 agcgcctggt ggcgggactg gggcgggaag acttcgaggt ctttgacgag gggcagcggc
    18481 aggagattgc ctatttcgga cgggagtcgg agccactcga cgttttgctg ctgttggacg
    18541 tgagcggcag catgcggcgc tacgtcgagg agatggcgcg ggtcgcgcgc gatgctctgc
    18601 gtcagttgcg cgccgccgac cgcgtggcgg tgatgctgtt cagccggcgg tccaaggtga
    18661 gcgaggagtt cacggcggat ttcacggagg tggcgcggga gatgagcgcg gcggtgcgag
    18721 agcaggggtt gggagcaggc acggcgatca acgaggctct tttggctgcg tcggcctatg
    18781 ccgggcggca gcccgcgcgg ccggggcgcc gagcgattct gatcctgacc gacaacctcg
    18841 gcctcaacta ccagacgccg gacgagcagg tggtccgggc gctttacgaa gccgattgtg
    18901 tactgaacgc cattgtggcg cgcggtggcg agcgtccgaa gccgccgagg ccgggcgagt
    18961 acctcaaccc ggacttcacg ccttcggatg tcttccggct cgcggaggag accgggggag
    19021 aggcagtcaa ggcggagcga gccggagccg ccttccagga gatgatggaa cgcctccgca
    19081 cgcggtacag cattcactac cgtgcgcccg aggcgccggc gggttcgttc cggcggatcc
    19141 gggttgcgct gaccgaagcg gcgcgcaagc gtttaccgag ggcggaagtg agggcacggg
    19201 cgggctacta cgcctccagg tagtgctacc ttgtgtccat ggatcggctg gaggaagtgc
    19261 ggcggaggca cggagcggcg gaggcgggcg ggggtgagga acggcgggag cggcaacacc
    19321 aggccgggaa gctgtcggcg agggagcgca tcgacctcct ggttgacgaa ggcagtttcg
    19381 aggaactgga caaactcgtc cggcaccgcg gtagggactt cggcatggac gagcaggtca
    19441 tcgacgggga cggcttcgta accgggtacg ggcgcatcga tggcaggctg gtgtacgttt
    19501 tcgcgcagga cttcaccgtg ttcggcgggt cactttcaga ggctaacgca cagaagatct
    19561 gcaaggtgat ggaccaggcg ctgaagaacg gcgcaccaat tatcggcctg aacgattcgg
    19621 gcggggcgcg tattcaggag ggtgtgcttt cgctcggcgg ctatgccgac atctttctgc
    19681 ggaacacgct ggcaagcggc gtagtgccgc agatctcagc cattatgggc ccgtgcgccg
    19741 gaggcgccgt ctattcgccg gcgatcaccg atttcgtctt catggtggat aagacgagct
    19801 acatgttcgt gaccggtccg gacgtgatca agacggtcac gcacgaggac gtcacgaagg
    19861 agaagctcgg cggctcggtg acacacaaca cgatcagcgg cgtcgggcac ttcatcgccg
    19921 ccgatgacgc cgaatgcctg cggatgatcc gtgagctgct tttgtacctt ccgcagaaca
    19981 accatgacga tccgccggcc gttccaaccc aggacccgcc ggagcgagag gatccgacgc
    20041 tggacgccat cgtcccggca gaatcgaatg tgccctacga catcaaggac gtcatccacc
    20101 gggtggtgga cgacggggcg tttctggaag tccacgagca cttcgcgaag aacatcgtcg
    20161 tcggcttcgc gcggatggag gggcgtacgg tgggcattgt ggccaaccag cccgcctttc
    20221 tggctggctg tctggacatc aactcgtcgg tgaaaggggc gcgcttcgtc cggttctgcg
    20281 acgcattcca cattccaata ctgaccttcg aggacgtgcc gggattccta cccgggacgg
    20341 accaggaatt cggcggcatc atccggcacg gtgccaagct cctctatgcc tatgccgaag
    20401 cgacggttcc gaagatcacg gtgatcacgc ggaaggcgta cggcggagcc tactgtgtga
    20461 tggggtcaaa gcacctgcgg acggacatca attttgcgtg gccgagcgcg gagattgcgg
    20521 tgatgggtcc cgagggagcg gtgaacatcg tgtatcggcg ggagctggcg gccgcggaag
    20581 acgaggcggc ggtccggcgg gagaaggtcg ccgaattccg cgagcgtttc gccaatccgt
    20641 tcatcgcggc cgagcggggt tttgtcgacg atgtcatcga gccgcgtcag acccggccaa
    20701 aagtgatccg ggcgctacgc atgctcgaga acaaagtcga cacgatgcct cggaagaagc
    20761 acgggaacat accgctgtag gaggccggct ggcggagtca aggtacacgc tcaggagcgg
    20821 tcctcctcga ggattcgtcc actgtcggtc ttcaccttgg ggaacctgag caggtaggtg
    20881 tcgcgtctgg gcaacgagtt accgagccga atcaaagaga cgggacgcaa ttccgccatc
    20941 gggactccac tcgacgacga tcacctcgcc ctcggacgcc tccttaacga tggcgccggt
    21001 gttcaggtgg agttcccgga tgctcacccg cctcatgcat cgcatcgtga cacagttttc
    21061 ctgtatgaag gttactcggg tcgaaaaggc ggcttccagg tatataatct agattatatg
    21121 ctccccttga aggtcacgac ggtgggaaag tcggccgggt tcatcctccc caaagaggcc
    21181 atgacacgaa tgaagcttaa aaagggagac atcgtcttcc tcacggagac ccgggaaggc
    21241 tatctgctca caccctacga tcccgagttt gaggcgcaga tggagatcgc ccaggagggc
    21301 atgcggcgtt accggaacgc ccttcgcgag ctggcccggt gagcaaagag cctgtgtggc
    21361 tcgaccaccg gctggttctg gcaatccacg ggcgcgtgct cgtggagcac ggcggccggg
    21421 ctggagtccg agacgagggc ttgctcgagt cggccctggc acggccgcga aaccttcgcc
    21481 actaccagag ggcgtccctg tacgagttgg ccgctgcgta cgccgccgga atctgccgga
    21541 atcaccctct cgtggacggc aacaagcgcg tcgctctgat ggccgtttac gtcttcctgg
    21601 ctcggaacgg ggtagtgctg cgtgcgggcg aggcggacgc agcggcggcg gttacgggac
    21661 tcgccgcggg aactctgcca gagagccagt tttcggcttg gctcaagaag aacacggaac
    21721 gaccggccag gcctgtgaag agagtcccag gcgggaggga atgaacgact actccgagtc
    21781 gccaccgacg cggaggacgg cgaggaaggc ttcctggggg atgtcgactt tgccgacgcg
    21841 tttcatacgc cgcttacctt ccttttgctt ttcgagcagc ttgcgcttgc gggtgatgtc
    21901 gccgccgtag catttggcga ggacgttttt gcgcatggcg gggatgttct cgcgggcgac
    21961 gatcttgttg ccgatggccg cctggagcgc cacttcgaac atctgccggg ggatcagttt
    22021 gcggagccgc tcgatcagag ctttgccgcg ctcgtaggcg aagtcgcggt ggacgatcat
    22081 cgagagcgcg tcgacagggt ccccggccac cagcacgtcg agttttacca tcggagattc
    22141 acggtagccg gcgagatggt agtcgagcga ggcgtagcca cgggagacgg acttcaggcg
    22201 gtcgtagaaa tcgaggacga tttcgttgag gggcaactcg tacatcagca acacacggcc
    22261 gacgccgatg tactcgaact ttttctgcgt gccgcgcttt tcctcgagca gtttcaggat
    22321 ctcgccgagg gactcttcgc gggtgatgac ggtcgcttcg attatcggtt cttcggtggt
    22381 ctcgatcaac gacgggttcg ggaacctggt cgggttgtcg acctcgacga caccgccgtc
    22441 cgtcttgacg acccggtagc ggacgcctgg ggcggtggtg atcaggtcga tttcgaactc
    22501 gcgttccagc cgctcctgga cgatttccag gtggagcagc ccaaggaagc cacagcggaa
    22561 gccgaagccg agcgcgaccg aactctcggg ctcgaagctg aaggcgctgt cgttcagtcg
    22621 caacttctcg agcgcttcgc gaaggagacc atgcgcgtgc gactccaccg gatagaggcc
    22681 ggcgaaaacc atcggcttgg cctcttgaaa tccgggcagc gggtccggag cggggttggc
    22741 gtcgtcgatg acggtatcgc ccacctgcgc gtccgagacc gtcttgacgt tggcggacat
    22801 aaagccgact tcgcccgccg tcaactcgtc gcacggaatg cgcttgggag tctggtaccc
    22861 gagtccctcg acttcgaaca tcttgccgct cgaccacagc cggatcttct ggccgacgcg
    22921 aagcgccccg tccaccacgc gcagcagggc gatcacgccg cggtacgggt cgaaccacga
    22981 atcgaagatc agggcgcgga gcggggcctc gggattcccg cgcggcggag gcaccaggtg
    23041 gacgatggcc tcgattgtct cgtggatccc gacaccattc tttgcactgg ccatcacggc
    23101 gtcgcgggta tcgaggccga tgacgtgctc gatctcttcc ttgactcgtt caggttcggc
    23161 ggaggggagg tcgatcttat tgataaccgg gatgatttcg aggttgtgat gcagtgccag
    23221 gtaggtgttg gcgagggtct gcgcctcaac cccctgggag gcgtcgacca cgagcaaagc
    23281 gccttcgcac gattgaaggc tgcgcgaaac ctcgtaggtg aaatcgacat gacccggcgt
    23341 gtcgatcaga tttaactggt aatgattgcc gtcatcggcg atgtagttga gccgcacggc
    23401 gtgcgcttta atggtgatgc cgcgctcgcg ctcgaggtcc atcgagtcga ggacctgctc
    23461 catcatctcg cgctcagaaa gagcgcctgt cagctcgagc aggcggtccg caagcgtgga
    23521 cttgccgtgg tcgatatggg caatgatgga aaaattccgg atgtaccggg gatccatgcg
    23581 gtatgatgtt gctctgaaga tcgattatcc catatgtcct acggctccat tgcgttcgag
    23641 ttgtcggacg acggcatcgc gctgatcacg attcggcgtc ccgagaagtt gaacgcgctg
    23701 gacgtggcga ctgtcggcga gcttgacgat gcgttgtcgc gcgcggcgtc cgatgcatca
    23761 actcgcggcc tgctccttgc cggagccggc gacaaggcgt tcgtcgcggg ggctgacgtt
    23821 cgggagctcg cggcgctgga cgcggcgggg tgcagagagt tctcgctgcg cggccagcgg
    23881 acgttccgga gactcgagac gatgggcaag ccgtcggttg ccgcgatcca tggctatgcg
    23941 ctcggcggcg gactcgaact cgcgatgtgc tgcacgtttc gggtggccgc gccggaggcg
    24001 aaactcggcc tgccggaggt gaagctcggc atcatcccgg gttacggcgg tacgcagcgg
    24061 ttgccgcggc tggtgggcag gagccgtgcg ctcgggatgc tgctcacggg cgagcccgtc
    24121 ggcgccgaag aggcgttgcg aatcggcctg gtcgatcacg tcgcgccaag ggcggaattg
    24181 acggagtttt gccgcgggct gctgcgcaag atgctggcca acgcgccggt ggcggttgca
    24241 cttgcgatgg aggcggtgga cattggtctg aacgcgggtg tcgaggaagg attgcgcgcg
    24301 gaagcggcag ccttcggact ggcctcggcg acggaggatc gcgcggaggg tatgcgggcg
    24361 tttctcgaga aacggccggc ggtgtttcga ggaaagtgac ttatggcaag ccggataatt
    24421 gaaggcaatc tggacgcgaa gggactcaag ttcgcggttc ttgtgacacg gttcaacagc
    24481 ctgatcaccg agcggctgct cagcggcgcc ctggacgcgc tggcgcgtac gggatgcggc
    24541 gccgacgaca tagagatcgt ccgggtaccc ggttcgtggg agcttccggt ggtggcgcgt
    24601 gaactggcgc tcctaaagcg gcacgacgcc atcatctgcc tcagcgcggt cattcgcggc
    24661 gacacgccgc acttcgacta catcgccgcc gaagcctcga agggtctggc gcagacgtcg
    24721 atggagaatg gggttccgtt ggccttcggc gttctgacct gcgatacgct cgagcaggcc
    24781 ctggaccggg ctggagcgaa gagcggcaac aagggattcg acgccgccat gaccgcggtc
    24841 gagatgggaa atctcatgcg gcggttgcgc gccggatcgt gagcggacga tgccgtcccg
    24901 ccatcgctcc aggcagcgcg cgcttcaagt gctgtttcag attgacatca ggcagcaatc
    24961 cgcagccgag gcgctcgatg gcttttacgg ctcgttgatc acgtcggagg aggcgcccgt
    25021 cgaatctgag gagcgcgacc ccttcatgga acggctggtc aagtctaccg tcgagcgcct
    25081 tagcgacatc gaccggcggg tctccggtca ttccgagcac tggcggatcg agaggatggc
    25141 cgctgtggac cgcaatatcc tgcggctggc ggtgtgcgag atgacgagca ccgacacgcc
    25201 ggcggcagtc gtgatcgacg aggcgttgga actggcccgg cggttttcgt cggacgagtc
    25261 agtggctttc atcaacgggg tactggacgc ggtgcaccga agctggactt ccgggcacga
    25321 agtcaccggc cctccgggtc ctgctccgaa aagccagtga acccggacgc agcagattcc
    25381 gtcagacgcc cggctctccg cagcgcgtcg cgcagcagga attcgatctg tccgttgacg
    25441 cttcgcatct cgtcatccgc ccagcggcgg agcagatcga acgtcctgcc gtcgatgcgc
    25501 agcagaaaag tcttgcgccc ttccatctcc acctactggt agatcgtccc ggcgttgatc
    25561 acgggttggg cggcgcgttc gccgcaaagc accacaagca ggttgctgac catggaggcg
    25621 cggcgctcct cgtcgagctc gatgatcttg ttccgggaga gcatttcgag cgccatctcc
    25681 accatgccga cggcaccttc gacgatgcgc tgccgggcgg caatgatcgc gcccgcctgc
    25741 tgccgctgca gcatcgccgc cgcgatttcc ggggcgtagg cgaggtggct gatacgggcc
    25801 tcgacgacct cgacgccggc gaccgcgagg cgttcctgga tttctgtctt caggtgttcg
    25861 gcaacggcct gtgtgtgacc ccgtagggag gtctcagttt cctcgtgtga gtcatagggg
    25921 taggcggtgg tgagattccg cagcgccgcc tcgctctgca cgtgcacgta gttctcgtag
    25981 ttgtcgacct ggaagcaggc ctcggccgtg tcgacgactt tccagaccac gacagccgca
    26041 atctcgacgg ggttgccgtc cttgtcgttc accttgaggt ggccggtctc gaagttccgg
    26101 acacggacgg agatgtgctt cttcgagaag aacgggttgg cccagtgaag gcccgagcgc
    26161 cggacggttc ccacgtactt gccgaagagc tggagcacgg cgccctggtt cggctggacc
    26221 ataaacagtc ctgcgaaaca gagcgaattc acgaccagaa caacgatgta aaggatgatc
    26281 agccacggct gttcacccct gggggtggtg cgcgcggtat cgataatccc gtagacgcag
    26341 agcgccgcga caacgatcag cgcgatcagc gtgaagactc cggggagggt agagcggggg
    26401 atttccttga tcatgaccat agcctcatat cgaaatgata tcacaatgat gtcaagagtc
    26461 aaggaggagg gtgtgaagcg cctttccgac gagtcgccaa agccgggatc agggctgggc
    26521 ctatgacaga tcgatgagga accggcagcg cgcgcccgcg tgccggcgcg gcgagtccgc
    26581 cacttcgata cggaccggct tgccgccgac cgtttcaaac aacaactggt tgttcgcctt
    26641 ggagcagtcg cagtaaggat cgtctggagt tggtgctcgc ttaggactcc agccgcagac
    26701 gcattgaccg gacggataga tgagggtgac caggtcgccc tcccggcggc agttctccgg
    26761 tcccacaatc ttccccagca actcaacgag tttgtccagg ttgccctggc tgtcctggat
    26821 gagcttttgc cggaaatcca gcgcacggca gcaggcgcgc ccccgctctg tcatcagctt
    26881 gatcagggta tcgcgatcca agctgctttc ctctttctgg aggaacgtgg agagaaagtc
    26941 ccgcacggcc ttggcgtcgg aggtgcactt ggcatcctcg cattttccgc tcactgtctg
    27001 cccaaggctt gaacagcaag gaaggaccgc tccggctgcc aggtttcgta gaaatgtctt
    27061 gcgatccatg atgccctcca tatcgggacc agaaggtccg atatgggtca gagtaacacg
    27121 gctactgctg tttggacggc gggcactacg gcagcgcctt aatgctcagc ctatttgccg
    27181 atgcagaagg tggagaagat gcggttgagg atgtcgtcgg cggtggtggc gccggtcatt
    27241 tcgtcgaggg ggcggagggc gccgtagaga tctaacagca acatttcatg gggaatgccg
    27301 ccgaggccgg cagtgtgagc cttgtcaagg gaggcggtcg tctcgcggag gagacgctcg
    27361 tgacgaatgc tggtgatgaa gccggaatct gcaaggtcac cgccctcggg cgcgagggcg
    27421 tccagaatcc ggcggcgtag ctcgtcgatc ccttcaccgg tcagagcgga gacgaccacg
    27481 gtgtcgatgt ccatccctgc cgcgcgaggc aagtcgcatt tgttgccagc cacgataaaa
    27541 cggccctggc cgctagcgcg ccggaccagt tcaagatcct gtgtttcgac cacgacggag
    27601 agatcgagga ccacaatggt caggtcggcg tcagccatcg cctgaaagct ccgctcaatg
    27661 ccgaggcttt ccacgagacc ttcgccctcg cggatgccgg cggtgtcgac gtacttcacc
    27721 ggaatgccgc cgagcgaggc gacttccgat acgaggtcgc gcgtggttcc ggggatctcg
    27781 gtgacaatgg cgcggtcctg ctcgactagg cggttgaaga ggctgctctt cccgacgttg
    27841 ggccggccaa ctatcgccag cgtcaacccg gagtgaacca accggccgta gccgaagctg
    27901 cttaacaacc ggccggctgc gtcgcggatc ccgtcgattc ggcgaaggat ctcctcggcc
    27961 ggtgcgacgg cgacgtcatc ctcggcgaag tcgataccgg cctcgagcaa tgcgatcaac
    28021 tccagaagtt gctccttcac cggcgccacg cggcgcgaga cagaccctcc gatctgctgc
    28081 gcggcgatgc gggcctggta aagagtggtg gcttcgatga gatcgcggac ggcttcggct
    28141 tgaggaaggt caatgcgtcc gtgcacaaac gctcgcagcg tgaactcacc gggctcggcg
    28201 agccgcgccc cggcgtcgca ggccttctcc agacagaagc gcagtacgac cggcgaaccg
    28261 tgcagggaga tctcgacgac gtcttctgcc gtgtaggagc gcggctgctc gaagaacgtg
    28321 acgacggcct ggtcgacagt gtgcccagcc tcgtcagcca attcggcaag cgccgacgtc
    28381 caggtgcgcc aatgaggacc gtcctggaac cggagaatcc gggccgcgat ctcgcgcgcg
    28441 ccgggtcccg aaagccgcac gacgccgata ccgccacgtc ccggcggtgt ggaaatggca
    28501 acgatggtat cgcgaaagtt caaatcaacg gcgacggcgg cggacgcccg gcgggcggcc
    28561 cggcagagcg gcgtggaact gatccggcgg ctctgagggc gcgccttcgg ggtagactac
    28621 aacctgccgg cggggccctt ctccggcgct ctcactgcgg acgccctgtt cgtcacggaa
    28681 cgccagatgg agaattcgcc gctcgcggct ggtcatcggg ctgaaccgga acgactggcc
    28741 ggtacgacgg accttgtcgg cggccgcggc cgcgctcagg cgaagctcct cgatgcggag
    28801 caggcgatag tcgttggcgt cgaaccgaag acgggagtgg tcttcaggcg gaacgcgaag
    28861 gatctccatc gtcaggtatt cgagcgcgag gagaagctcg gcgcgattcg cgaggagatc
    28921 gtcgacgtcg gagccagaga atcggacgac aacgtcgggg tcctcgatct cagcgtactg
    28981 accgtcgtag tcggagactt cgaacttcag gtggaaaccc gcttttttca gaacgggctg
    29041 aagaaaagcc tcaacacgcg gagctaacga ttcggtggtg tacttccgtg tgcccacgag
    29101 ctatttcttg gcgcctttct tgacttgctt taccggcggc gggggcggca ccggtgcgac
    29161 cgaggtcttg ttgaagaacc actgctgcgc gatgccgact acgttgccgg tcaaccagta
    29221 tagcaccagg ccgctcgaaa ccccgtagaa catgaagccc aggatgagag gcatcatcag
    29281 catgatgcgc tgctgcgagg ggtcggcggt cgtcgagggt gtcatcttct gcatgatgaa
    29341 ctggctgacg atcatcgcga tcggcaggat gcggatgggc agggtttccg gctgcgacag
    29401 atcgctcacc cagagccacg acgcgccgcg catctcgatt gccaccgtga gcaccttata
    29461 aaaggcaatg aagaatggga tctggagcag catcggcacg caaccgccca tcgggttcac
    29521 accgtgcttt ttgtatagct ccatgacctc ctgattctgg tctgccttac gggggtcacg
    29581 gatgccgatg cccttgtact tttcgttaat ggctgcgatc tgaggctgga gcatctgcat
    29641 ttttttcatc gacttcaggc tcgtgaactt caacggcagc agcatgaagt tgatgatgat
    29701 ggtcacgacc acgatcgacc agccatagtt ctgcacccag cggtcattga cccaattcac
    29761 cgcgaggaaa agcggcttgg cgaggaagct gaaccagccg aagtcgacta cctgttcgag
    29821 cttcggattc accttacgca ggatgtcgat gtccttgggg ccgacgaaga tcggcatccg
    29881 gttcgcgccc gagccgccga cgctcatgcc gatgtggggg acttccttat ctttctgttc
    29941 gcccgccaca tggtcctggt aggtgaagac ttcggtcgaa acgcccgagg cgggaaggaa
    30001 caccgcggcg aagtactggt cctcgatacc ggcaaaggaa aacgtgccaa tggcggagag
    30061 agggccgtct gaggccgcct ttgcttcttt cacgatgagc ttgttcgccg tgacgtcgaa
    30121 gtggagggta tgttcggtgg cggggtgatt tgggactgtg ccgtcaccga aaccgcctcg
    30181 ccaagcgatg tagtgcggga gggacttgcc atcctgggtc acttcgctcg atacgtcggc
    30241 caggtagctg tccttgcgga atcggaatga cttccggctc cagaccctgc cgttggagaa
    30301 ttcgaagtcg actccgagcc cgtccgagac gggtttggca gcgtagagca cctgcttcag
    30361 gtcggcggct gccttgccct tgaacacaag ggagaaggga tagcccgccc gggccgcccc
    30421 ggcggcatgg accagatcga gcggtttccc ggcgccgtcc ttgtactttt tcaggatcca
    30481 actccggact accgcacctt tattcgaaaa cacgacgcgg gcgaggtcgg tttcgacgat
    30541 gtaggtttct tctttttccg cacctacttg ccctgcggga acagcaggca gcggagcctc
    30601 aggcgcggta gcggcctgct ccacaggggg cttcgcggac ggtttagccg ggctgtcctc
    30661 agaggcggcc ggtttgacgg gcgccttcga ggaaggaggg gaggaccggt agaagtaggg
    30721 cgtcagcatc aggacgagtc ccatcaggag gaaggcgagc agcatcctcc gttccatgga
    30781 aagccccttc tgtcctccgg gcttgccgcc agtcttggcg ccgttttcag gatcgtccat
    30841 gtgatcttaa ccttgtgaca aatcgggtgc gggttgcgca ggtcatcgga ccggatcgaa
    30901 acctccggca tgaaacgggt ggcaacgcac gagccgcttg aggccgagcc acacgccccg
    30961 gcgggcgccg tatttctcga ccgccgcgcg catgtagtcg gagcaggtcg gatagaagcg
    31021 gcatgcggag ggcagaagcg gagagaggca gcgcttatag aagcgcagga gaaacacgac
    31081 taacgcctgc atcgcaagaa gagcctctcc acctcccgct tgaggtcctc gaaggaggcc
    31141 tcctgggcgg cctttcgcgg gttaatcacg atccaccatc ccggcccgag cagttccaga
    31201 ctgagccgca ccgcttcgcg catgcggcgt ttgattcgat tccttccggt gctcttaccc
    31261 agagccctgg gcgtcgtgaa accgaccctg gaccgctcgt ctccgctcgc gttcagacaa
    31321 aaagcggcga agaacggtcc ggcgacccgg aaaccctgat cgtacactag ccggaattcc
    31381 ccggtccgta ggagccgaac ctccttgggg aaagagcgat ctctgctttc ggctttcttg
    31441 gatctactct gcgtaacgaa cggctctctc ggagctgacg gtcaacttct ggcggccctt
    31501 cgcccgccgg cgggcgagga cggcccggcc gtccttggtt ctcattcgta cccggaaccc
    31561 gtgtgtcttg gcccgctttc gattattcgg ctgaaatgtg cgtttgggca ttttcgtcac
    31621 cagcgccaat caggacaagc ttccccggcg ccccgcttcc atgtagaatc tagactttcg
    31681 tcggaacttc cagtataagg gtgcggaaaa accagtgtca aaccaagaga agagcagcgg
    31741 cggggactgt aacggcgggc gtgtgcgtga cgtcggacat cacgtgagat accgacgagc
    31801 ggcctttctt cttctgatcc tcctgattgc ggcggatttc tccgcgctgc tggctcagcg
    31861 aggctttgac gagtacgcac tcgtgctcca ggacccgcca ctggcggcgc aggttccctc
    31921 ccgaaaagag ctgcggcgtg ccgccgccga cgacggactg cggcggatcg aggcggcgca
    31981 gtcccagatg cgcgaagaac tcgggcggag ggggattcac gtctccggtt cagcgcggat
    32041 cctggtcaat gcggtgttcg tgcgcgcccc gaaagagaag ctggcggaat tgcgggcgtt
    32101 gcccggggtg agccggctgg agtacatgcc gccactccgc ccgctgttgg atcgagcggc
    32161 ggagctgata aacgcgccgg cggcatggaa cgccctgggc ggcacggcga acgccggggc
    32221 cggcatcaag atcggcattc tggacactgg catcgaccag aaccacccag ggttccgcga
    32281 ccccagcctg acggcacccg ccggctttcc gaagtgccag ccgcaagact gcgcgtatac
    32341 caacagtaag gtgatcgtgg cccgaagcta cgtaagtatg ctggcggcgg ggtttggaga
    32401 cgatgtggcg gcgacgtccc ggccggatga tctgagtcca cgggaccggg taggccacgg
    32461 aacagcagcg gcgatgatcg ccgcgggggt tcggaacacc gggccgagcg ctacgatcac
    32521 gggaatcgct ccgaaagctt acctcggcaa ctacaagatc ttcggctcgc ccggggtgaa
    32581 cgactacacg ttcagctcga tagtgattca ggcgctggac gacgcgttcc ttgacgggat
    32641 ggatatcgtg acgctgtcgc tcggcagtcc tgccggctat gggccgctgg actatccgtg
    32701 tacctcgtcg accgggcaac cgcagccgtg cgatcttcgc gccgacgcgg tacaaaatgc
    32761 ggtcaacaag ggcatggttg tggtggccgc cgccggcaac gacggcgaca ttggactcca
    32821 gttcccgaca ctgagcacga ttgctacgcc cgggacggcg ccggcggcaa tcacggtggg
    32881 agcttcgacg aattcgcacg tgttttttgc cagggtgaag ctcaccggcg gggatgcacc
    32941 gcaaaacctt cagcaaattc cggctctgtt cggggacgga ccacatccct tggcggctct
    33001 tacggcgccg ttgaaagacg tctcgaagct cggcgccgat ggcagggcgt gcaatccgtt
    33061 gcctggtggg tctctcgcga gttcgattgc actggtcgag cgcggcgact gctcatttac
    33121 gcttaaagtg aacaacgctc agaccgccgg cgccaccggc gtggtcattt acaacaatgt
    33181 ggattcgaac tacccgccct tctccccggt ggggctggtg ggtacgggca ttccggcagc
    33241 cattatcggc cggaatccgg gtctcgcgtt aaaggacttc ctcgtggcgc acgccgaccg
    33301 gccgacgacg atggatccgg cgctcgttcc gcaggacgcc gattacgata cagtggcttt
    33361 cttttcgtcc catgggccgg cgattggcac gaacggaatc aaaccggagc tggtagccgt
    33421 tgggaccgac ctctacaccg ctacccagac atacgacccg aacagcggtc tgtgggaccc
    33481 gaccggctac acgccgatgc agggaaccag tttctccgtt ccgatggtag ccggcgccgc
    33541 ggcgctggtg atgcaggcga gaccgtcggc tcaggcggac gacgtgaagt ccgccgtggt
    33601 aaacacggcg atgaacatca tccaggacga tgttgggctc gctcgtgtga ccgccgtggg
    33661 ggcggggata cttgacgcga acgccgccgt acgttcaacg gtttcggtgg acccagcaac
    33721 gctctctttc ggggtgatcg cgggatcatt gccctcgacc acgctaagaa tcacaaacac
    33781 cgggatgcag ccggtgaacc tgaccctggc tgtcgaaccg cgtgacaccg acgccaacgc
    33841 acgggttgta ctggatcgca gcagcctgcc cctcgccgca ggggcgacaa ctacggttgt
    33901 ggcgcggctg gagggtactc agccgggccc cgggtcatac gaaggggccg tcacgatcac
    33961 cggcggcagc gtggccttgc gcgtgccgta tctctatctc gtgggcgacg gaattcccta
    34021 caacatgttt gtgatgttcg gtgacgagtt cgacggaacc cccgggggag agctcatcgg
    34081 gcggttaatc ggcttcaagc tgatcgataa gttcggcgtg ccggtggcga gcgttcccgt
    34141 cgtctttcgg tcgacgctgg gtggagggac gatcaagagt gccgatgcaa atacggatat
    34201 ctacggcatc gcggcggcca ttccagagtt aggaccacag atcggtcagc agcagttcac
    34261 ggcaacggcg ggcggcttga cagtcgattt taacggcgag gccttcccct cgccgtcgat
    34321 ctataccaac ggaatcgtaa acgcagccag cgggcggatc gaccaggggc tggcgcccgg
    34381 atcgtatgtt tcgatttacg gccaggcgtt gagcccgtcg ttcctggcgt tcgacagcct
    34441 ccccctcggc taccttccgc tctccctcac gaacgtgagc gtcagcttcg acgtgccgga
    34501 ccgcgggatc agccttcccg gccgtctgca tttcgtcagc gacggacagg tgaacgtcca
    34561 ggttccatgg gagcttgagg ggctgaactc ggtcaaggtg aaagtcagca tcgccgatat
    34621 ctcgagcgcc gtttacgata tgccgctggc caggaattca ccggggttct tcgagtaccc
    34681 gctgggcagc gggactgtgg cggctcttgt gtcccgtaac gggcagtact cggtgatcac
    34741 cacgagcaac ccggccaggc gtggggagac cgtggagctt tactgcaacg gtctcggccc
    34801 tgtgaacgac acgcctccga ccggcgaggt gactccggcc ggcgtactgc gaaggacccg
    34861 ggacacggtc tcggtgacga tcggcggccg gccggcgacg cccgacttca gcgggctcac
    34921 cccgtacagc gccggtctct accaggtgaa cgtgaaggtg ccggacgacg ccggcgttgg
    34981 aaaccggccg gtagtgatgt ccgtgaacgg cgttaccgcc aagacggtga acctgccagt
    35041 ccagtaggga aggtcttcat gctctataat gagcgtctgg gagaagtctt cccttcatgg
    35101 aagaggtcaa gaaaaaactg caagaggaga tcgccgcact cgaatacgag ttgcgcaacg
    35161 agcttccgcg ggaaattctg aaggcgcgcg cacacggcga cctgagcgag aatgccgagt
    35221 accacgccgc caaggagcgg cagcgctatg tgagcgcccg gctgagccag ctacagaaac
    35281 ggttgggcga gctttccatg gtcgatctcc gaaatctccc tcaagggcgg gtaggccttg
    35341 gttccaaggt ggttgttctt gacttgcaca aagaccggga aatcgccttt aaacttgtga
    35401 ctagcgaaga ggcggacgcc tccaaaggct ggatttccac cagttcgcca atcggcaaag
    35461 gtctgctcgg caaggaactg ggggatactg taaaggttca gattccgggc ggagtccggg
    35521 aactggagat cgtgaagctg accaccgtcc acgacgcgga tgagtagcgg cgggagagaa
    35581 ttgtaccgcc catgacgtac acacgcgcaa tcggagtcgc ctgcaacaag gtcatcaggc
    35641 tgatggtgcg aggactcgcg ctcgcgcgca tccatccgaa cgttctgact ttcatcgggc
    35701 tccttatcaa cggagtagcg ggcgtgctgc ttgcttccgg acggttcgtt gcggctggtc
    35761 tcgtaatcat catcgccggt atcttcgata tggtcgacgg gcgggtggcc cgggagacga
    35821 accaagtcac tgcgttcggg gggttcttcg attcggttct ggaccgctat tccgacctgg
    35881 tgctgctcat ggggatgctg gtgtactacg catcgatcga gcggtttttc tacgtggtgc
    35941 tgacggcggt cgtgatgaca ggatcggtga tggtgagcta cacacgcgcg cgggcggaga
    36001 acgcaatccc gcggtgcaag gtcgggttca tggagcggcc ggagcgcatc gtgctgctca
    36061 tcatcggagc gctgttcaac cggatggcgc aggtactgtg ggtcatcgcg gtgctctcga
    36121 acctgacggt catccaccgg cttatcttca cgtggcagga aacgaagcgg ctggaggaag
    36181 cgcaactcca gccgacgaag tccactgaga tggcggagcg caaggcgtaa ctcctctacc
    36241 cgccttccat gatcacaaag gctacggcga cggcggcggt gtgcgtaatt gaaagcgaca
    36301 ccgatttcac gccgagccgt tcggcgaaac gggcggccac gccgtggagc agaagggtcg
    36361 gcttgcccga gggtaggttg gcgacctcga agtcgcgcca gcgcacgcca tgcctccagc
    36421 cggtaccgat ggccttcatt ccggcttcct tggcggcgaa acgggcggcg taccgttcga
    36481 atcggttggc tttccgctcg acgtaggcga tctcgcccgg ggtatatatg cgctcgatga
    36541 atcggcggcc gaaacgctcg atcgcggccc ggatgcggtc cacctcggcg acgtcgagac
    36601 cagtaccggc gatcacagca cggctcccga gccggtgatc gtgaggcgac cgtgcttgac
    36661 gcccttggtg ctgatcaggc tgtcggcgat cttgcggacc accgaggcgt tgcccttgac
    36721 caccaggacc tcgaggcagt tgtcgtggtc gaggtggaca tgcagggcgg acagaatatg
    36781 gtcaaagtac ttgtgttgta ggtcagtcag cttctcgttg agcaacctga cgtggtgatc
    36841 atagacgaga gtgaccgtgc cgatcaccgg actgtccggc gcctgccacg ccttttcgac
    36901 aagttcgtcg cggatgagat cgcggaacgc ctccgagcgg ttggtatacc cacggctgag
    36961 aatcagcgca tcgaattttt cgaggagatc ggtgtcgatg gccacgccaa tccggcttag
    37021 ttcgcccatt tgtaggtcag gttacagcag gcggcgtggg gcggcaagaa ggtggcacgg
    37081 ttctttggat cgtgctacct tgagagcgtt ccgatgcaca tccccgacgg atttctctcc
    37141 acttcggtct gggccacgat gagcgccgcc accgtaccat cggtcgcgta catggtgcgg
    37201 cgcgctcagc gtgaggtgga agaggcccgg gtgccgttac tcggggtgat gggggcgttc
    37261 gttttcgcgg cgcaaatgat caactttccg gtcgggatcg gaacgaccgg gcacctgatc
    37321 ggcagcgcgg tattggcgta tacgctcgga ccggcagccg cgagtgtggt catgacggcg
    37381 atcctcgcca tccaggcgct cgtgttccag gacggcggtc tgctggcgct cggcgccaac
    37441 gtattcaata tggcggtggc gggtgtgctg gccgcctacc tgccgtatca ctactggggc
    37501 gcgggacggt ggcggaaggc ggccattttt tcgggcgccg cgctgtcggt gctgacgggg
    37561 gcgttgctgg cgttggcgca gttgcgattc tccggcgtgg cgatgccggg ggcggtcgtg
    37621 tgggtctcgc taggtctgtt tacggtcaac gctctgctgg aaggcggaat cacactggcg
    37681 gtcatgcaat cgctggaggc gctgaatccg ggctggctgc gaaagccggc ggcgagcggg
    37741 cgaacggcga tgggtgtact gctgaccgcc gccgtgttgc tcgtaggcgc cggcgtgttg
    37801 tttgcggcga gcgatccgga cggtatcgag aaactggcgg agaacctcgg catcgcatca
    37861 catgcgcgaa ctctgctgga gacaccgcta ggcggctacg agatacagta cctgtcctcg
    37921 ggctggctgc gaaaggccac cgccgggctg gcgggcctgg cgctgatcta cggcgcctgc
    37981 gtagtgttcg gcaagctgat cgcacggcga aggagcgctt gattgcacca cgtcgtactg
    38041 gaccgctgga gccggggcag gagtttcctt caccaccgcg acgctcgcgc gaaggtcctc
    38101 gcactgctgg ctttcctgat tattgtggcg acgacgccgc ccggttccaa cgcggcggca
    38161 gccgggtacg gcgcattcct gttggcggcg gcgctgctgg cagggctccc ggccgggggc
    38221 gtgctgtggc gcggcgccgt ggtactgcct ttctccgcgg cgttcgccgc aatgagcctc
    38281 ttcgcgggcg acaacatacg ggcaatagcg cttcttgaga agagctacct ttcggcactg
    38341 gcggtggtga tcgtcgtggg gacaacaccg atgcctgaac tcctgcgagg cctggagtcg
    38401 ctcggggtcc cgcggttcct tctgctcgtc gtccaattcc tataccgcta cctgtttgtg
    38461 atttctgagc aggcgcagca catgagagtc gccgctggag cacgcggaac ggcttcaacc
    38521 cttgcggttt tccgccggtg gcggctccgg gcagcggcag gagcgatcag cgtgctgttc
    38581 gcgcgctcct acagcagagc cgaaggcatc tatcgggcca tggccgcgcg aggtttccgg
    38641 gggcacatct ctttgctcgc cgcgaagcgg ttccgatggc aagacgtggc gttcctggtg
    38701 gcgtcggttg tggttccggc cgccgtgcga atcgcgctcg gagcaggagg cggatggccc
    38761 cgctgatcga agtcaaagac ctgcgtttcc gatatcccga cggaacgccc gcactcaacg
    38821 ggatcgattt taggttgtac ctaggcgaga cggtggcgct actcggcgca aacggatccg
    38881 gaaagacgac gttcgttctg cacctcaacg ggatattgca gggcgagggc ctggtgagcg
    38941 tttgcggcat gcaactgagc aaggagacgc tgccggatat ccggcggaaa gtcggcctcg
    39001 tatttcagga agccgacgaa cagttattca tgccaacggt actcgacgac gtcacgttcg
    39061 ggctgctgaa tctcggcatt tccccggagg aggcggccac gcaagggcga gaggcactgg
    39121 ctcgcgtcgg gatggaaggg gcggggggaa aggcgcccta tcacttgagc gccggtgaaa
    39181 agcggcgggt cgcactcgcc ggagtgcttg tcatgcggcc agagatactg atcctcgacg
    39241 agccgaccac ctacctggat ccgcccggac agaggaacct cttggaactc cttcgtgagt
    39301 tgcctcaggc caagatcgtc gtgacgcaca gcacgacctt ggccttggct gtttcacaga
    39361 gggcggtgtt ctttcaaaaa ggaaagatcg ccggtgaagg agtgacggaa gagatcatcc
    39421 agcggtttca gtgggatgcc aggcagcctt gccgaggaat ccacggaggt tgaaaccgga
    39481 ggacggtttc gttaggccga agcggcctgt gccttccggt actccgccca acgccgcttc
    39541 tgtgcctggg caatgcgctt gcgggccgct gcgctgaggc ggcgttttct cgccggtttc
    39601 tccgcggccg gacccgaggg agagtttgac tttaccctcc tggctccgag cagggcgcgg
    39661 acttgttgta tctgttcctc gatccgctgc ttctgcaact ccagaccttc cagggccgct
    39721 gcgtagatcg atgtatcttt gagtgctttt gtgactctgg ccattttgtc tcccgtcatc
    39781 caaaataaca tatccgcgac ttagaagtgt caaaccgact tttccgaatc gaggatgaaa
    39841 gttaccggac cgtcgttgac cagatggacc gccatttctg cctggaaaac tccggactgg
    39901 accgagaccc ctcgggtgcg gacggaatcg atgaagtact cgtagaggtt tcgagccagt
    39961 tccggcgccg cagcccggtc gaaacccggc cggcggccct tccgcacgtc cccgtacagg
    40021 gtgaattgag aaacgaccag catacttccg ccgatgtcga gcaagctgcg gttcattttg
    40081 ccctcttcat ccggaaaaat ccgcaggcag acagtcttgt cggcaaggta gtcggcgtcg
    40141 gccctgacgt cagtccgcgc gatgcccaca agtgcgacca atccccgctc tatggaaccc
    40201 gcgacccgct caccgacttc tacgcgcgct tcgcttacgc gctgaaggac cactcgcata
    40261 tggtataagt atggcagaat ccgctcatga ttgagactta tgcatatgca cgcgccggcc
    40321 tgctcggcaa tccaagcgac gggtatttcg gcaagactat ttcgttactc gtcaagaact
    40381 ttcgcgctcg tgtgttgctc tatccaagcg cccggcttga gataagaccc tcgaaggccg
    40441 acacgccgat attcgaaaat ctgcaggacc tgtacagcac cacgcgctgg cggggatatt
    40501 acggtggaat caggatcatt caggcgctgg tggtccggtt catcgattac tgccgcgagg
    40561 taaattttcc cctggaagac cgtaacttca cgctggagta cgaatcaagc atcccactcc
    40621 ggctggggct tggcggatcg agcgcgatca ttacggccgc tctgcgcgcg ttgtgccagt
    40681 attttgggat cgagattccc aagccggttc aagccaacct tgtgctggag acggaaacca
    40741 aggagctcgg cgtaccggcg ggacatcagg atcgcgtgat ccaggtctac gaaggcctcg
    40801 tatacatgga ttttgcgcgc gagctgatgg aaacgagggg ctacggaaac tacgaaaacc
    40861 tcgatcccgg gctgctgccg agcatctacg ttgcataccg caccagtctc agtgagggga
    40921 ccgaaatctt tcacagtagc gttagggaac ggtggagaag gggagatccc gaggtggttg
    40981 aagccatgcg tgcgtgggcg agctacgcgc aagaggggcg tgaggcgctg ctgcgccgcg
    41041 atggcgacac gctggcgggg ttgatcgacg ccaattttga tcttcgcgcc cggctctacg
    41101 acatcagcga cggcaacctg cggatgatcc gcgtcgcccg cgaggttggc gcgacttcga
    41161 acttcgcggg atccggtggc gccatcgtcg gcacgtatcg ggacgagcgc atgtacaagt
    41221 cgctggtagg tgcgatgaaa gagatcggtg tggcggtgat caaaccgaga gtgacgtagg
    41281 acccgtggac cggtcttgct tgagtgtttc agtcgcatcc gacggtttat gttgaaatga
    41341 aagctcggta ccgagcatgg cggccaggaa aataatccag tataaagacg taggggtcaa
    41401 catagacgaa gcggatcgcg ccgtcgcgca gattcgcgta atggcgcgga agacgttcac
    41461 gaggggcgtg ctgaccgaca tcggcagctt cggtgcgggc tatcgcctgg cggggtggaa
    41521 acagccggta ctgatcagct cagcggatgg cgtggggacc aaagtaaagc ttgcctttct
    41581 caccgggagg cacaatacgg tgggtgagga cctggtgaac cactgcgtga acgacgtcgc
    41641 tgtccagggt gcaaccccgc tttttttcct ggactacttc gcggtgggcc gactcgatgc
    41701 cggcgtcgcg gcgcaagtgg tgtcggggct ggcgcgcggc tgcgaggcaa acggctgcgc
    41761 gctgatcggc ggcgaaacgg ctgagatgcc ggggctctat gcccccggcg agtacgatct
    41821 ggcggggttt atcgtcggcg cagtcgagcg ggtgcgcctc gtcacgggaa gagacatccg
    41881 cgcaggcgac gtgctcctcg gactacactc cacggggctg cacacgaacg gatactcgct
    41941 cgcgcgaaag gtgctgctgg aggtcgcgga gtaccagccg cacacgttcc ttcccgaact
    42001 cggccgcacc gtcggggacg aattgctaca ggtccaccgg agctacttgc ggccattgca
    42061 ggcgctcttc acgaagggcc tcctcaaggg cgcggcgcac atcaccggtg ggggaatcac
    42121 ggagaacact ccgcgaattc tgccaaaggg cctggccgcg gagatcgaca ccgcccggtg
    42181 gcgcgtgccc acggtgttcg agttgctccg gcgcatcggc aacattcccg acgacgatta
    42241 ccggcggaca ttcaacctcg gcatcggaat gatcctggcg gtttcgaccc gaaaggcggc
    42301 ggaagcgaag cgcacgctgg accggctcgg cgaagagcat gtagaggtcg gccgcgtcgt
    42361 accgctgccg cgcggggcaa aaggccgggt tctttacata tgaaacgtct tggcatcctc
    42421 ttgtcggggc gaggctcgaa cttcgaggcc atcgccgaca gcgtagtcac gggccggatc
    42481 gacgcggaga tcgccgttgt tctctcgaac cggccggaag cccgcgggtt ggaaatcgcc
    42541 cggtcgcggg ggttgaatgc cagctgcatc ccctccaagg ggctcgacag ggaagtctat
    42601 gaccggatgg tggtcgagga gctgaagaag agccgggtgg acctggtgtg cctggccggc
    42661 ttcatgcggc tgctgagcgc ctacttcatt cgcgagtttc cgctacgcat cctgaacatt
    42721 cacccctcgc tgctgccggc gttcccaggg ctcgacgcgc agcatcaggc gctcgagcac
    42781 ggcgtcaagc tgtccggctg cacagtccac tttgtggacg agaacctgga ttccggaccg
    42841 atcctcattc agaccgcggt cccggttctc gacgatgaca cagccgactc tctttccgcg
    42901 agaatcctca agcaagaaca ccggatttac accgaagcga tccgcctcgt gctctccgga
    42961 aagttccgca tcgaagggcg tcgtgtaatt gccggatagc tccacgcgcg gctccacgtt
    43021 ttaccgttag gcgaatccac cgccgccggc cgccatactc aagacagcac acgctttcct
    43081 tgcgcaccgg gtaagaacga tacctcggcc ttactccttc gtacttccgt actcgctata
    43141 agaagcccat tctgggttca accgggcggc gagccagcgc cggtaacact gttttccatc
    43201 gaaggtcgtg agcgccggag ccgcccgaag gaagggggtc aaatgcgttc aatgacgacg
    43261 gggattctgg ccgcggcgct gctgcttgcg gcaaacgcga caagcggaac cggcgacgac
    43321 aggcccgccg cgggacaatc ggttaaacca gaaaatctgc acgggatttc ggcgagttcg
    43381 gccgcggcca agtctctgaa cgacctggga gctctccatt atcggcaggg ccgatacgaa
    43441 caggcggaag aggcttacaa gaaggcgatc acgatctggg agcaggggcc agagagggaa
    43501 agcccggagg cggcgtcgac gatcgacaat ctgggggtgc tgtaccgtgc acaagcgcgt
    43561 cttggagaag cagcggagct gcaccgccgg tcgctggcta tgcgagagcg tctgctcgga
    43621 ccggagcacg gcgacgtcgc cgtgagcctc gccaacctcg ccgaggtcgt gcgcatgcag
    43681 ggccacgccg gcgacgccga gccgttggca cgccgggcgc tggcgatcgc ggaacgggcg
    43741 ttcggtccag agcacgcgaa gaccggcttc gcgctcgccg ttcttggggc gatctatcgg
    43801 gagcagcagc gttggccgga agcagaaaca gtatacgagc gggccgccgc cgtctccgcg
    43861 gccagtctcg gcgaaaacag cgccgcggtg gccgagaatc tgaacaacct tgcgacggtg
    43921 tatatcgcgc agcgcaggtt caccgaagcc gaggcccagt tgcggtggtc actggaggtc
    43981 tgggaaagat cgctcggccc cgctcatccg agcgtggcgg tcgcgctgaa caacctggcg
    44041 caggtggagc ggctccaggg ccgctacggc gaggcggagc cactctaccg ccgggcgctg
    44101 gagattctgc aacggacgaa cggccgcagc cacccgagcg tcgccaaagg ccttcgaaat
    44161 ctcggggctc tgttcttcga gcagggaaaa ctcgcggggg cggcggagct ttaccggcag
    44221 tcgctggaaa tctccgaagg ccttgagggg cccgagcagc ccgaggtcga agcctgccgg
    44281 gaagacctcg cgagggtgta tcgggcacag ggccggatga gcgaggccaa agcgctatgg
    44341 cgcggttcac tgggggcccc gaactcgctt cgggaggccg attcgggcga gcagcgcttg
    44401 aaagcggggc gctgaacgca gctcgtcgta gatgggatgg acaccgatgt tagccagggg
    44461 gagctcgcgg cgctcgcatc caagctccag ccagtcgagc gcccgctcct tctcgccgag
    44521 acccacatgg atgagagcaa agcacgtggt aggcgcgtac ctctgtccgg acatggcacg
    44581 cagttcttcg agtagagcta cggcaccggc gcgatcgccc gccaaagagc gattgtgtcc
    44641 gagggctgcc aggatactcg gcacgtcacc tccgagcgaa cgcccttttt cgaacatctc
    44701 ggcggcctcg cgatacttgc cctggaggct gagcgcccgg ccgatggcag tgtaagcctt
    44761 gtagaaattc cggtccagtt ccaacgcctt ctcgtaacat tcgatcgcct cgtcgtagcg
    44821 gcggccaagc agcgcaaggt atccaacccc ttcgttgatg atcagcgaga gaggatcgag
    44881 gcgcagagcg atgtccatct cccggcgggc ttcctcgaag cgggcctgca tggcgaggaa
    44941 gtcgatggca taccagtggt gcgcggtcgc gtaacccggg ttgagcgcga tcgccttccg
    45001 gtaatgcttt tcggccgcgt cccagcgcca gtcatggaga gaacggatga agcccagcga
    45061 cgtatgggct tcggcgagcg tgggatcgat ggcaagcgcc ctccgggcgg cgcgcctcgc
    45121 ctttttcata atttcggacg gctgggccag gccgtagtcg gccagaaggc tataagcgtc
    45181 cgccaaacct gcatagccga gggcgagtgc ggggtccgcg gagatggctt gctcgaagag
    45241 ccggatactc tgccgcagac catcgggtgt gcgcttgtgc cagtgaaacc ttcccagaag
    45301 gtacaggttg taggcggcca cgtcggcgga ggcgcggcgt tcggatacgg aggcaggctg
    45361 ctccacaagc ttcaatcgca gtgtgtcggc gatcgaccgg gagatttcgt cctgtatcgc
    45421 gaacacatcc tgaagttttc gttcccacgc ttccgaccag agataccggc cgtccgatgt
    45481 gctcaccaga tgcgctgcga cgcgcagcct gtcgccggac cggcgcacac ttcccccaac
    45541 ggcggcgtcg accttcaatt gttcaccgag ttgcttcagg tcgtacgact tccccttgaa
    45601 ttgcgatgcg gactgccagg ccacaacgcg aaggcccgcg atccgggtaa accggtgaat
    45661 cagctcttcg gtgaggccat cgctgaagta ctcgttctcc tggtccggtg tcaggttggt
    45721 gaacggcagc acgacgatgg cttgcgggac gtcacccgcg ggagattcgg ggggtgaccg
    45781 gtcaggatcc agtgtggagc ccgcccggcg ggcgaagcgc ggcacatagc caccgggctg
    45841 gatctcgatc acaatgtcgt cgtggcggcc ttcacgttcg tagtacgcgg taagtttcga
    45901 acgaagccgg cgagcctcga cgcgaacgat cgggtcagtg cgcggatcgt acgagggctc
    45961 gcggtcgaac acttcgacgc cgagcaaaga ctccttcaat tcgtcggatc ggccgtcgag
    46021 cgcctggtcg acggcgaagc gcaggaaacg actgatgcgc ccggagcgga caaaagcggc
    46081 gctgtggagt atccgctcca gttgagcgcg gacggctttc ggcgatactc ccgcagcggg
    46141 ccgccgcgcg gcggccgctc ccacggatgg ctcgtccatt gcagatccct gccaggcaag
    46201 agtgccgcgt ccgaggccca cctaccaacg cggccttttg cgttctacga tcttattatg
    46261 caacgaagga cctttctcgc cgctaccgcg gcatcctacg cgcagattct cggcgccaac
    46321 gacaacatcc ggatcggggt gatcggcagc ggaggccgcg gtcgcctgct gacgtcagaa
    46381 ttcaaagaag tcggggcgga gatggccgca gtctgcgacg tctacgaacc gcatctgcag
    46441 gccggagtga aggcggcctc caccggcgcg acggcataca ccgactaccg gcgcctgctc
    46501 gatgacaagt cgattcacgg cgtcatcgtc gctactccgg accactggca tgcccagatg
    46561 gcgatcgacg ccgtcaacgc cggaaaggat gtgtacctcg aaaagccgat ggcgcacacc
    46621 atcgacgagg ggtttcggat cgtggaggcc gtccggaaga ccaagcgggt cgtccaggtc
    46681 ggcatgcaac gtcgtagcta tgacgtgttc cacaacgcgc atagcatcat gaagtccggg
    46741 caggttggcg aagtgcgact ggtcaattcg tggtggatga actatactcc ctcgctgcgc
    46801 ggcgcaaacc ttgacggcaa gctcgactgg aaccagtggc tcggttcggc gcccaagcgc
    46861 ccgctggacc caatgcggtt ccgaaactgg tactggttct gggattattc gggcggaatg
    46921 ctcatcggcc aggcggcgca tgtaatcgac gccatccaca tgctgatgga ctcaacgttc
    46981 cccgcggcgg tgacgtgcgc ggccggcaag gtagacgtgg acggggccga ggttccggag
    47041 actgccacaa tggcggtcga gtacccggag aattacctag cggtattcac cattggctac
    47101 aaggcgatgc ggtaccacac gttcaacgac cagatgaaga acttccacgg atccaaagcc
    47161 cggttcgacg tgtggcggga agggtacgcg ctgtatccgc aaagtagtgc aatcgagatg
    47221 cggccgaccc tcgaattacg gaaaccaggg agctttgtgc cggcgacgcg ctcacacatc
    47281 cgaaatttcc tggactgtat ccgttcccgc aaagacccga acgcgaccgt cgaaatggga
    47341 aactacacaa acgtggcgtt gtgcatggcc atgcagtcac aacgcgaggg ccggcggctg
    47401 cgctggaacg ctgcgtcgcg ccgagtggag agctgaggtg cgctatttcc gtaacaggaa
    47461 gttgccgacg aacattgcgt cgattcccga cgagtagaaa ctgcgcacgg cgtcccgcgg
    47521 cgtacagacg agcgggtctc cgaacaggtt gaaggacgtg ttgtagagca cgggcaggcc
    47581 ggtggccttg ccggtcgcgt gcagcagttg ccagtagagc gggttatcct cgcgggacac
    47641 cgtatggacg cgcaccaggt ccgcgccgag gatggcgccg tcgaagagct ttttgtgctc
    47701 cggcttgacc cgggccacag tggccaggaa tcgcgcgttc gggccgacct cgaagtaccg
    47761 atccgccagt tcggcgggca ccgaggcggc gaatttccgg aacggctccc ggtgcttgat
    47821 gtaggtgttc aggttctcgg tcgagtaggc gtcaagcggc gaggcaagaa tgctgcggtt
    47881 gcccagtgcg cgcgggccga attccatgcg gccatgcatc caggcgacga tccggttgtc
    47941 ctggatctcg cggacagcgg tctccagcaa ctcctcggtc gtcagcaggt aacggaaacg
    48001 gagcttgcag ttctccaaca cccgcttgat ctcctcagcg ccatagctgg gacctaagca
    48061 gaaatcgcct aggccgatcc gggaggtctg gccatagaca tggtgccagg catggtacac
    48121 cgcgccgagc gcagtgccgg cgttgcctgc cgcaggttgc acaaaaacgt tgctccagcg
    48181 cccgctgcgt tcgagcgcgg ctaccagcag cacgttgaga gcgaggccac cggcgaagca
    48241 gagattctct ccttcgccgg ccatttcgcc tactgtctcc tcgatcgcct gctgcagacc
    48301 agccgccagc gagggcttca tcgatggcgg gatctcggcg ccgtcgtcga ggtctaaacg
    48361 ttggtagaag cgggcgctga aaccgccatg tccctggcgt tccccgtgga agaaagaacg
    48421 gtcgatgcgg ggccagtttc cgttgcggag ggccaagatc tccagaaaga gttcgtggta
    48481 gcggtcatcc gggcacgtgg ccgaaagcca ctggacctta tgctcatcag cattggcctc
    48541 gaagccgagc agcgcggtaa cacggccgta gaggtccccg agcgagtccg gatagtagag
    48601 ttcacgctcg agggtgagct ggtttccgga agcacgccag cgggcgccac accggaagtc
    48661 gccgccccgg tcgagggtga gtaccgtcgc ctgatcgaag ggcgaggcgt agtaagccga
    48721 agcggcgtga gcgacgtggt gttctacgag aacaatctgc gcgttgggga aggcccctcg
    48781 cagttcgaga tggatggcgg tttcgacgcc tgacgagaag ggccgaacaa gggcgacgca
    48841 gtcgaccgcg gctgtcggca cgccggccat ttcgagacaa atcgcgatgg cttcgtccgg
    48901 gagctcaccg gggtggaacc gccgggcaag tttcttctgt tcgacggcgg cgaccagccg
    48961 gccgtctttc acgatggcgc aggcggcgtc attgagcaag ccgccgattc cgaggatgac
    49021 cattcttaaa ctcccatggc ctttcaggtg tgaccgtctt accgcttctt ctcctccaac
    49081 gctttctcga tcttggccca ggtatcgcga agtgtcatcg tccggtcaaa caccaggtgg
    49141 tccggggctg agtccgaatc gacgcagaag tagcccagac gctcaaactg gtaccggctg
    49201 ccgtgcccgg cgccggccag gctcggttcc agcttgcagc cggtcaagac ttccagagat
    49261 ttcgggttga ggtaggaggt gaaatccagg ccttcactgt cgtcacccgg gttctcgcgg
    49321 ctaaagaggt ggtcgtacag ccgcacctcc gcctcgacgg catgagcggc agacacccaa
    49381 tgaatggtcg ccttcacctt tcgcccatcc ggagcgttgc ctccccgtgt cgcggggtcg
    49441 tacgtgcaat ggatttcggt cacctcgccg gtggtctcat ctttgacggc gccggtgcac
    49501 ttgacgaagt acccgtagcg aaggcggact tcgcggccgg gcgccagccg gaagtactgt
    49561 ttcggcggat cttcgcgaaa atcgtcccgt tcaatgtgga gcacgcggga aaaagggacc
    49621 ctgcgagtgc ccatgcctgg atcctccggg ttgttgaccg cctcgagttc ctcgacccgg
    49681 tcctcggggt agttgtcgat cacgacccgc aaggggcgca acacggccat gacgcgcggg
    49741 gaacgacggt taaggttctc tcggacaaaa tgctccagaa gagagatctc gatcacgccg
    49801 tccgtccggg ccacgccgat acgctcacag aaagcgcgga tcgcttccgg agtgtagccg
    49861 cggcgccgga tgccgcagat ggtcggcatg cgcggatcgt cccagccgct cacgtgcccc
    49921 tgttggacca acatcagcag cttgcgcttg ctcaacaggg tgtgggtcag gttgagccgg
    49981 gcaaactcat actgtctgga cgggaagatg cccaactgct cgatgaacca gtcgtacagc
    50041 ggccggtgat ccgcgaactc gagcgtgcat atggagtgcg tgattccctc gatcgaatcg
    50101 cactggccgt gggcgtagtc gtacatcgga tagatgcacc acttgccgcc tgtccggtga
    50161 tgttcggcgt gaaggatccg gtacatcacg ggatcccgca tgttcaagtt gggcgaagcc
    50221 atatcgatct ttgcccgtaa ggtgcgcgag ccgtccggaa actctcctgc ccgcatgcgc
    50281 tcaaacaggt tgaggttctc ctcgaccgag cggccgcgat aggggctttc ccgtccgggt
    50341 tcggtcaacg tgccgcggta ctcgcgaatc tcttccgcgg tcagatcaca gacatacgcc
    50401 ttaccggcgt tgatcagttg tacggcccac tggtagagct ggtcaaagta gtcagaggca
    50461 tagaagagcc ggtcttccca gtcgaaaccg agccagtgga cgtcttcaat gatcgagttg
    50521 acgtactcgg tttcctcctt gctggggttg gtgtcgtcga acctcaggtt gcatttgccg
    50581 ccgaactccg cggccagccc gaagttcagg cagatcgatt tggcatggcc gatatgcagg
    50641 tagccgttgg gctccggagg aaaacgcgta tgcacccggc cctcattctt gttggcggcg
    50701 acgtcttgac ggacgatctc cttgatgaaa tgggaaggag aagtcgcggc ccctgattct
    50761 ttcctggcat cctcaccaat cgtgtcggga tttggcatac tcatcgcatt tccactccga
    50821 ttcagacagc atgctccgcc agcttatgta tcgcctggcc gatccgcttg aggcaggttg
    50881 ccctccccag cacttccatg gtttcaaata gcggtggcgc cgctttgcgg ccgcacaccg
    50941 ccacgcggat gggttgaaac atctgccccg ccttgactcc catcgcgttc gcttcgtcac
    51001 gcagcctcac ttcgagcgcc tcgcgtgtga aagcggccgc gtcgagggct ttgccggcac
    51061 gctgcaacgc ggccagggcc attgccgcat tccccttctg tggaatcaac tcagaggcgt
    51121 cgtagggcgg tagttcgtcc atgaaaagga agttcgccac ggttgccaca tcgccgagcg
    51181 tggtgatgcg ttcccggatg agcggagtca cctgaagcat cttttcccgt tcgacctgaa
    51241 agccggcggc gcgggcgaag gggagcaacc gttcagcgag ttcctccacc ggcagctgac
    51301 ggatgtaatg gccgttgaga tgatccagtt tgtcgtagtt ccagacagcc ggcgatgcgt
    51361 tcacacggtc gagactgaag cgctcgatca gctcgtcgtg gcccatgatt tcggtcttgt
    51421 cgtcgtaact ccacccaagc agggcgaggt aattgatcat cgcctcgggc aggtaaccca
    51481 tctcctcgaa ggtgcgcacg aacacgggca gcttgcgccc gtcaggcgct cgatcctcgc
    51541 gcttgctcat cttccccttt cccgttgggt tcaggataag cggcaggtga gccatcaccg
    51601 gcggctccca accgaaggcg gtgtagagat ggatgtgaag gggcagactg ttgacccatt
    51661 catcgcctcg gaggatgtgg ctgatctcca tcaggtggtc atcaatgacg tttgccatgt
    51721 ggtacgtcgg gatgccatcg gacttcatca gtaccgcatc ctgcaaattg gcgttctgga
    51781 acgtgatgtc gccgcggatg acgtcgtgaa ctgcgatctc cccgtccagc ggcagcttga
    51841 atcgcaccgt gtgaggttca ccggctgcgg ctcgccgggc tgcttcctcg ggatccaggt
    51901 ttcggcagag gcggtcatag ccggggttta accccttcct ctgcaactcc tttgacacgg
    51961 cttcgagccg ctccggagtg cagaaacacc ggtaggcggc cccggcatcc agcaaccggc
    52021 cgcaatggcg ccgatagacc tcgatgcgct gggtctgaac gtagggaccg tagtcgccgc
    52081 cagcctccgg accctcgtcc caatcgagtc cgaggtagcg tagcccattt aagagccatg
    52141 cgagagcctg cggattgtat ctcttctggt cggtgtcctc gatgcgaaga atgaaactgc
    52201 cgcggtgatg gcgggcgaac aggtagttga acagcgccgt tcgagcgccg ccgacatgga
    52261 aatctccggt tgggctgggc gcgtaacgca cccgtacagg cttcaacggt gtcgtcatta
    52321 tgacttacgg cctcggcaag agggctgttc acaaacagga aagacagcga ggcgtcaaat
    52381 ttcaagtata gggtatcggc aatcaacctt gagcgcgcaa gcggcggtag agagcggcga
    52441 gttcgccgac ggacaactgt tcggcccgtt gcgaggcttc gggtgttccg tcgagtaagg
    52501 tcttaccgaa gaggccggca aggttgtttc ggattgtctt ccgtttctgg cggaaacatt
    52561 ggccggcaaa ctcgaggaaa gaggacggat cgtctatacc ccactgctcg gcgacagcga
    52621 gcggaccaaa tcggactacc gccgaatcga tcttgggcgg cggacgaaag gcagcggccg
    52681 gaacggtgaa cagatactcg ggttccgagg acaggcgggc aagcacggtc aggaaaccgt
    52741 agtcgcgggt accgggaatg gcgacgagcc gaagggcgac ctcgcgctgg accagaaata
    52801 cagcgcgccg aagcaacggt ccaagatcca gcaccttccg caggatcggc gaggtaatgt
    52861 agtacggcag attgcctgcg aacacggccg ggccccactg ggagagatca gtttccagga
    52921 cgtcgccgtg aacgcgttcg aggttggcgg ttcgagcaaa cttctcggag agacgtccaa
    52981 tcaggattgg gtcggcttcg atcgccacta cccgcgcggc gcgcgcgagg aggtgccggg
    53041 tgagtgcacc ggttcccgcg ccgatctcga ttaccaaggg ctccgggtcg ggacacgcca
    53101 cctccgcaat ccgatgcagg acggaatcct tgtgaagaaa atgctggccg agtcttcgcc
    53161 ccaacgcgcc gccgtcagcc ccgcacgtcg atctgggtgc gtcggaacgt caggcggagc
    53221 ttaccggaca aaggctgtcc gaagttcgtg gcgggcgaaa agagggtgaa aaacagggcg
    53281 ttctcgacga tcttgcggct ctccggatcc ttgacccacc gctgcccttt gggggtggaa
    53341 tagtcgacca tgcggccttg ctcgttcaca gtcaggtcca ctacgatgtc ctcgtcgctc
    53401 agggtgaacg gccccatgct gataaatgcg gcctcggtgg agaggatggt cggcacgtcg
    53461 tccactctca tattctccgg aggcgcgaac acaggcgtca taacggcgaa caggaccaca
    53521 gcggatacca gaccgccggc gaacgggagg gcaagcggcc gcatgatgtt ttcgagccag
    53581 agcctggccg tcccgaccaa ctcaccgacg aggccggcga aggtgccacg acggagacgg
    53641 cggatccgct cccgtgacgc cagcacggtc agcctggtag tcaagtgatt cgggggagtc
    53701 cgtaccggca aggcccgcaa agccgatcgc atcaattgca gcctttgtgc gagtgcggca
    53761 cagtccctgc aaccggccag atgcccggag atgtccaccc tttcccgcgc cgatacgacg
    53821 tcgtcggcgt aggccgaaag ggaacgctga accctgtggc aattcatacc atcactccac
    53881 agtctgcggc atcaaccgga aggccggctc agattccagc cgcgtttcga gttcccggcg
    53941 cagcgcgtca cgaccacgca ggatccgtga cttcaccgta ccgagaggca cctggaggat
    54001 cgctgcaatt tcctcgtaac ttagctcctc cacatcacga agaacgacaa ctgagcgaaa
    54061 ggtcgggttg aggtcggcga gtgccgcctc gatcaacccc tgccgctcac gatcgagtac
    54121 gtaatcgaac ggcgagcggc ccgaggccgg cagggtgtca cggtagctgc ggccgtgttc
    54181 gggacagctt tcgaggtcga cctcctggtg acggtggcgg ccattccagc gacagtggtt
    54241 gttggtttcg ttgacagcaa tccggtagat ccacgtcttc aagctgctgt ctccccggaa
    54301 caaaccgatt tttcggaaaa ccttgagaaa cacctcttgc accacgtcgc aggcttcgtc
    54361 gggatcccgg agcagacggc tcaccaggtt gaagaccggc tgttgaaagc gctggatcag
    54421 ggtctcatag gcggcctccg ctcccagccg cagtccttca acaagacgtt cgtcctccac
    54481 tgccacggct gcctcgcgtg gggcggcgat ctcctccagg agatcgggag tttcgacact
    54541 cgcaagcgat tcggtcacgg tagcgcctca accggcaatt tcactttcaa actaccacga
    54601 tccactacgc cctgcaaaag atttgacgtc aaatggcggc aaaagttccc gtagcacacg
    54661 acggtgccga aaacggcttg atgccgccca atagccactg tcgcctgaca caggcgcagg
    54721 ccgcccttcc agtcgcgcga acacatggaa ctagttttgt tttcaaccga ttatcaggat
    54781 gcaacccatc gggagcagac cgcttcgtag tactgttcgt acatcggaat cacctgcgaa
    54841 ctggcgaatt cgttcaaagc ggtctcccgc gcggcttggg acatccgctg atggagtgcg
    54901 ccatcggtga gcagcgccac gacgcgggcc gcttgtgagg ccacgtcgcc gaccggttcg
    54961 aggaagccgt tcacgccgtc ggttacgagt tccggcacgc cgcccacacg ggtggccacc
    55021 ggcggcactc cgcaggccat cgcttcgagg gccgccagcc cgaaagcctc tagttcgctc
    55081 gggaggagca gcacatgggc ctgtgggatg acacgctcta catggctctg ttttcccaga
    55141 aacgtcacgt gtttctctat cgccaattct cgtgctagag cctcggccgg accccgctcc
    55201 ggaccgtccc ccaccatcag aaggtgcgcg ggcgtctcct tgcgcacttc gtaaagaatg
    55261 cggacacagt cgagcacccg cttcaccggc cggaagttgg agatgtgaat caacagcttg
    55321 tccccgagcg gagagtaata ccgggcttcg tgccgctccg gctccggctg gtacatctcg
    55381 caattgacga agttcctgat gacccggatc tcatttgtga cgccgaatac ctccaccgtc
    55441 tggctgcgga gatactcgct gactgtggtg actccgtcag aacgctcgat ggagaaccgc
    55501 gtgataggga agtacgatcg gtctgcacca accagcgtaa tgtccgttcc gtgaagagtc
    55561 gtgacgaacg gaagccggcg cttcgcggcc aacatatcgc gggctagcat ggctgagatc
    55621 gagtgaggga tcgcgtagtg tacgtgcagg agatccagat tgtagccctc ggccacctcc
    55681 gccatgcgcg aagcaagagc gaggcagtag ggagggaact gaaaaagtgg gtaagtgctg
    55741 acctcaacct cgtggtagtg gatacgcggg atgcctggat caagccggat tggattcgcg
    55801 tagctgatga agtggacgtt gtggccgcga ttggcaagtt ctaaaccaag ctcggtcgcg
    55861 actatgccgc tgccgccgta cgtcggataa caggtgatgc cgacattcac ttcgtcaggt
    55921 tagcattaac cggacggaga accgccaagc cgatctgtcc gttcaggttt tcgtcgggcg
    55981 aagcgacgtt gccgcctcca aaccagacgc ggatcagacc gtcgtccacc tcgactgagg
    56041 gatcgcagac gactttggag ttccaggatt gtccgccggc aactacggga aagtcgtccg
    56101 cgcgcaccca tcggacgcca tcgggggatc gggccagacc caaacggcgc tgctcatgcc
    56161 ggtcacgccc tgtatagagc atccagtaac tgccgtggga tgcccagacg gcaggttctc
    56221 cgagtccatt ctcatcgaaa gttcccggag ggcccggttc gaggatcggg ttcgctcgga
    56281 gtttctgcca ttcgaccccg tcgaaggagc gggcgatgcc aagccgctgg cggcgcgcgc
    56341 gatcctggcc gagatagtac atgtagaatg cgccggcaga ctgaacgacg tacgggtcgg
    56401 ccacgccgcg ttcgtcccaa ctgcctcgcg ggcccggtcg aaggacggga gcgcctgttt
    56461 tcacccaatg ccgcgcgtca gtggaacggg cgagtccaat cgccggccgt cggccccctt
    56521 gataccagta aaggaattgg gaaccggaac gggacaaaga accgttggct gcaatgtaat
    56581 ccccctccca ggaggcggga tcgggggaga ggacctggcc caggcgggtc caaaccacgc
    56641 cgtcgtccga tagggccagg ccggtacgcc acgaacgccc gtcgaaaccc gagtagagat
    56701 tcaggtaacg atggccttcc ttcacaaccg agggattgag ggcgtcaact gcatcccaag
    56761 ctccgtccgg tccccgtgtc agcacgggtt ccctcatcgc ttcccaccga aatgaggcgg
    56821 aggtacccgg ctggccaagc gtcggcaagg cgaagtcggc ataacggccg cagctgaagg
    56881 caaacaaagt gagcccagcg gcccccgatg cggccgctat ccgcgtgaga ctcatacaga
    56941 gactcggatt ttgagacgag aacctcgacc cggcttggag tccagcacaa gttcgcctcc
    57001 gagcccccgg acgcggcgcc ggatactgct cggaccgagt cgcaactgat cgagttcgtc
    57061 gagagtgtag catccggcaa acgtgaaacc cgccccgttg tcttcgacgg cgatttccag
    57121 tcgggtgggg tcgtgggtca cggcgaccgc cgcccgggag gcgccggcgt gtttccgcac
    57181 attgtgcaac gcctcacgga cgatttgaag gacctccatg gcggttgctg gagcgatgct
    57241 gtcagttcgg tcgttaccct caaaagtgac gtctattccg ctgtccttct gaaattcagc
    57301 cacaatgcgc cggatcgata cggacagtcc gcgctcctcg atcggtaccg gcctcatgcc
    57361 gcggaggaac gcccgcattt cctggacctg ggccttaatg aattcctgga tgtcctgaag
    57421 ttcgctcatg ccgccgtgaa agtcccgttc gatgagccgg cgcaacacct cgaggcgcat
    57481 ctgcaagctg acaaagctct ggagcgggcc gtcgtgaaaa tcaccggcga tccgctgccg
    57541 ttcatcctca cgggcggtcg cgagactcga tcgcagcatg tcagcctcgg cggtccgggc
    57601 cgatagactc tgccaaaggc gccgtttccg aagcgccagc aggcacgcca gcatgccccc
    57661 aggcagaata atccgccaga ggtctcctcc gtatggcgga cgtgcgatcg caaagaaccc
    57721 cgcagaggcc ccaacgacaa tgaaagggtc ccaccattcg tgcgtcacta ccgccgacag
    57781 caggagatag acgaagaacc cggaaacaag ccagagggag tggtctgatc cgaaaccggc
    57841 gaatacgagg aagaagatcg tgtcggagac gaggctgagg aggtcgccgc cggcctttcc
    57901 ggcggagcgc cggctcagcc cggcaaggct gtacagcgta aacgcggcgg ccagcacggt
    57961 cacgccagaa cgggcagagg cggcggaatg gaactgtacg ccgacactgc agaccgccag
    58021 cgcgaggcgt gtccacgcca gaacccaccg ccaagcgtct acgtctgcat tgtcgttgcc
    58081 agccaagttg cgaaccatac acgggcccgc tcgggtgcga ccgcccgcgc cctacgtcaa
    58141 accgagagcc ctacgccacg ggcccggcct atcgccggta aaggtaaagg gtgtgactac
    58201 gaccctcccc gtcgtcttcg atctgctcca ctttctccgg agtccaaccg gagaactcaa
    58261 aatcgtgtgc cacgatgcga gtccccttct tgagttgttt ctcgagcaag ggacggacct
    58321 tgtcgttgga ggacggcagc agatacacgg tgattaggtc agccgaactg tagtcctgaa
    58381 gcatgatgtc gccgtggatg atgcgggcag tcttctgcag gttcaggctc ttgatcttgt
    58441 cggtgctttg tctccaaagg tccttgtcga actcgatgcc ggtggcgtcg gcatggaatt
    58501 tctgagccgc catgatgacg atgcggccat cgccactgcc aaggtcgaac atcttctcgc
    58561 ctgccttcag gccgcccaac tgtagcatct tgtcgacaat ggtctcgggc gtcggatagt
    58621 acggagccag cggatcggtc tgttcgttct cctttaccgc ttggccgaac aggccgacac
    58681 aaacaatcgc gggaagcaga tatttcatcg ctctgatcat tagctaccct cggaacctat
    58741 gcagtgtaac ccaaaagacg tctgccggcg aagatccacc gcggcaagga atcagggttc
    58801 cagataaatc cttacccggt tgctttcctc gcctcccgcc tcgacgtaca attcggcggg
    58861 cccgaagttg acgatggcgg gcaggcggat ttcaaccagg tagaagccga catagccggg
    58921 agcgagggag gcgcgggtca cctccaccgg cgaacggtcg atgtagacgc gaacgggagc
    58981 gacgaccctt gggacatctt cgagcggagc agccatcccg gtcggccagg atgggcggac
    59041 tcgcccaaga cctgtcgcca ggatctgcac gcggctattc gaccgcgcag gattcatcgc
    59101 gtccagcaac gtaccgccgt caccgtcgat cagcattggc gtaccgtccc gatctacgaa
    59161 gattgccggc gcgactttcc ggagcggaaa attcagcgaa taccggcctc cggacccttc
    59221 cagtgaaagc tgagcagact gcccatccaa ctcgaaaggg atctggatct gggattcgag
    59281 ggcggaagcg tgcaacacgg gtgcatcgac gtatccggcc tgcgcacgca agagcctggc
    59341 cccgagcacg ctgagaagcg tacccggcgc ggctggccgg ctgctgtaat cggcggcgtt
    59401 tacgactcgg atgttgcggt agcggtgcgg agccggagcg ctgaagaccc cgtacccgcg
    59461 cagcgccacg aacaattggt tgcccggcgt atcaagagcg acgtccatcg cggggacggc
    59521 tggaaggttc tcaccgaccg gaatccacgg gcttgcgggg ccggcggcgg taaggtccac
    59581 gaacgtgagg tacacacccc ggtccgtcgc ggcatagacg gctccagcct ggcggtcggc
    59641 ggtgactgca ttcgcagcgc cctccggaag tccccctccg atgtcgtccc agaacacacc
    59701 gccgttcacc gtcctcaaga ttcgaggcga ggacgtctcg gcggaggcgg gaccgagcgc
    59761 cgccagcgcg agccgtgatt cctcgggatc cacgtagatt ccgcgaaccg ccccgccacc
    59821 cggcgggcgg gtcagcgtcc aggtccgccc ctgatcgagg gaaacccaca ttctgccatc
    59881 ccgcgctccg aggtaggagt aaagacggcc gggtgcgatt gccgtgacat ccgccgatac
    59941 ccggctcccc gccgcccgca agagatcttc tttctgccgg atggagggtt cgtccacgag
    60001 ccgccaggcc tccttctcgc cgggagccca ttccgcagcg ccagcgccat cgagaacgac
    60061 ccgagtgccc cggcttccct cagggagggt gagcaatcgc gccaccggaa gattcggcaa
    60121 tgtatcgttc atgccgctcc atgacattcc gccgtcggcc gatcgccaga tcccctggct
    60181 gttggctgca acaatctcac gcggatcacg cggggaaacg gctaggtccg ttagaccctc
    60241 accgatgacc gactcacgcc ggaaacgcgt caggttgaac caatgccgcc cttcgtcgtc
    60301 ggaccggtag acgtcgcggc cgatcccgta cagttgtgag ggggatgagc gggcgcgaaa
    60361 aatccggccg gtggattccg gcatggagac ggcgaaatca gctgccgagt ccccttcggg
    60421 aggggtttcg acgtccgccc gcagcttcca gttctcttga tcactggact gaaaaacccg
    60481 gccgtcaggc atccggagga aaacgctgct gccgtcctca gagaaccaga cgcggtcgac
    60541 cggccccccg gctccggagg ccaaacccag gtcagcgacg gaggagccga tccttcgcca
    60601 atccggccgc aacccaccgg gccgcctgga gtcctgctgg ccgaggcagg tggcggctaa
    60661 cgccaaggca aaaaaggcga tagccagcgt gcgcctcggt accaatagtg gggcgttacg
    60721 acacatcgat cgagtctatt ttatcccttt ccgggccttc ggtgctcaca aatcgtttca
    60781 ttttcttcag ttgcgcaaga aaaactcatc cggtggtcta cttgcaaagc aattttggtg
    60841 atagactgtg gtcttccggt cgcagccgaa gtgggccgtc gccggagcac agggtagtat
    60901 tatgtggcgc gggaccaaaa cctgcttcgt tgccacgctt gtctgcgtgg cgttggcggg
    60961 tgtgtgtctg gcgcaattcc cactcacgcg cacgacaagc cccggggctg ccaaagttgt
    61021 ctcagtcact ggacaggttt cggcgctgcg ggattcccaa ccatgggcgc ttggcgtcgg
    61081 tgacaccgtg cagttgcagc aggtcatcat gaccggagct gacggctacg ctgtgttcca
    61141 ggtttccgac ggcagtactt tcgaggtcta tccaaactcc gagatcgtct tccggcgtac
    61201 cccaggcaac tggaaagact tggtggacat cctcatcggc cgcgtcaagt tcacgatcca
    61261 gaagtttggc ggccagccga accacaaccg cgtgattacc cccacggctg tgatctcagt
    61321 gcgaggcacg gtattcgaag tcgaggtaga ggacgatgag gcgaccaccc tggtccaggt
    61381 tgacgagggg caggtagagg tccagcacgc caagcacccc ggcaagccga agatattgaa
    61441 cgccggcgag tggctgcggg tgtacaagga ccaaccgatc gcaaaatcac cggtggacaa
    61501 gggctcggtg ttcaacgccg ccgtccgtgc actttcggac gcactctaca ctgcggttta
    61561 tcgaaatcgc attccaactc cggcaggttc gccgattccc ggcagcgggg gtggcatccc
    61621 cctgcccggc gataccggga ccacgcctcc gcctccgccc ccgttgccgg gtgacggcgg
    61681 cggtacgaca cctcctccgc ccccgcctcc tccgccccca ggcgcataag ccgtgcgaca
    61741 ctagcctgta ggaaaggaga aaaacggcga atgacccgtt ggctcgggct ttggtgtctt
    61801 ttcgcgtctc ttggaggggt gcccggttgg gctcaggcgc ccgaacttcc tccggagtgg
    61861 gaggtgagcc gcgacatgac cgctcttgcc gaacagatcc gccgtttcaa gcccatcctc
    61921 gacgaggtga aaccccagga ttgggtatcc cagggagcgc cggaggccta ccagactcag
    61981 tggaagtcga tcacaagcga gatcgaatat ctgacccgtt ccaccaaaga attgtccagc
    62041 cagcccgagc ggctcacggt ggcgctcgaa acgctcttcc ggatgcaatc tctcgactcg
    62101 atactctcct ctttggacga aggcatccgg agataccaga acccggcgct cgccgatctg
    62161 cttcgcggcg aagcgaatca gaccgccgcg caccgcgaga aactgcgcca ctatgtggtc
    62221 gcgctggccg cgaccaagga actcgagtac aagatcatgc acgaggaggc tcagcgctgc
    62281 cgcgggatgt tgtcccggca gcctgctcca aagccaggcc caagccagcc ggcggagaag
    62341 aaatgaacga ggcagtcact ttctcatgca gcatctgtgg tgaggcgtct acacagatct
    62401 gtgtctggtg caccaaggac gcctgcgcaa accatctctg cgagaaatgc caccgctgca
    62461 gcgactgctg ctcctgcgag gaaccgctgg cgatctccga tcatgtcccg gagcaggacg
    62521 cagagaaagc ctgacggcct accggcggag attgtcaggc cgcagaccct cgtcgcttcc
    62581 cgcgcccgag tccagccact cttggcgcca ccgaaacacg ttgcccgtga gacttccgat
    62641 gaagatttcg cccgaccggc tcacgtcaat cgagtggccg gggctctcca tagaaccaag
    62701 aattcgtcca gtcgcgatgt cgatgcgcat gatgcgcccg gccagagtgt cgtaagtaag
    62761 gttctcgatg ttgttccggt gggtaatgat ccacatctcg tccagagcgg tgacgaatag
    62821 gccctgggtt gcgccgaggt gcgtccactg ccgcagaaac tttccgttcg cgtcgaagat
    62881 ctggatgcgg ttattctcgc ggtcgctcac ataaaccagg cccttggaat ccaccgccac
    62941 cgagtgaggt aggttgaact cgccaggccc cgtgccgcgc cgtccccagg cgagcaaata
    63001 cctgccgtcc cgcgaaaact tcaccacgcg tgagttcccg tatccgtcgg acacgtagaa
    63061 atcgccgttt ggagcgaagg cgatgtccgt cggccggttg aacgttttgt cgtccgctcc
    63121 cgcctgaccc cgcgtaccga gtcgcagaag aatccggccg tcattcgaga acttgatcac
    63181 ctcgtggttt ccgttatcgg taatccagac gttatcttcg cgatcgacgc ggagcccatg
    63241 gggattcccg aacattcccc ggccccagga gcgcaggtac ttgcccatcg catcaaacac
    63301 caggagcggg tcggccttgg ttcctcggtg gaagacgaac acttggcccc gggaatcggt
    63361 cgctaccgcg gacacacgcc cgagtttcga gccctcgggc atcgtgtccg gttcttctcc
    63421 gaagcggatt tgcacgcggt atttgagaag gccgccctca tggtcatgcc aaccctgctg
    63481 agccggcaga cacaccgctg tcagaagtgc gagcaccact ccagcgaagt ctctgtgtgt
    63541 caagtcatac atgaagattc tcgtttcagt ccactcttgt tggaatcttt aatatcctaa
    63601 ctcttgatgg tgcgcaagct gatgctcttt gttcctctga tggccatcgc ggcgacaacg
    63661 ccgagaattg tggtgcacgg ccaccgcggc gcgcgcgccg tcctgccgga gaacacgatg
    63721 ccggccttcg agcacgcgat cgaagcgggc gccgatcttc tcgaactcga cctggcagtc
    63781 accaaggacg acgtactggt ggtctctcac gatccgatcc taaatcccgt gatctgccgg
    63841 agcctgggcg gcgccactgt gatccgggag ctgactctcg ccgagctccg ccgatgggat
    63901 tgtggctcga tgcgcaaccc cgagtttccg aatcaacggc cggcgccggg gacgcgggtt
    63961 ccaactctgg acgaggtgtt gagtcttgcg ccgcgaggca gctttcagtt caacattgag
    64021 accaagatgt cgcccggcaa gccacagtac acgccgtctc ccggacggtt cgcggaactg
    64081 ctgctggccg ggattcgcaa gcgccatctc gaagatagag tgatggtaca gtcattcgac
    64141 taccggactt tgcgggccat gaaacggttg gcgccgggaa tcaggctctc ggcgctattt
    64201 ggagacgatg cccgcgactt cgcggcgatc gcccgggatg ccgggacggg catggtctcg
    64261 ccacaccagc ggttggtcac tcccgaaaaa gtggccgccg cacacgcagg cgggctgaca
    64321 gtgcatgcgt ggacggcgaa caaacctgag gaatgggacc ggttgatctc tgccggcgtc
    64381 gacggtatca taacggacga cccggcagag cttgtgcgtc accttcgcca acggggccta
    64441 cgttaagctc atcgccgccg gcggcccgct gcccgagctt tttcgagcac cgcttcccgc
    64501 tccccgtcgc tccatgcccg ttggatctcc tcctccgcca cacccgcgat gcgccccgcg
    64561 aggatgaaag ccggtagcgc gtgtcccagg ctcttccagt gagccatttc gatcaccgaa
    64621 tccatcgcgc tcgttctgat ctgatcgaga acctcagcgt cgcggctctc tgtcagcgtg
    64681 accagggcga aggcagcctt gttgcggtca gtccatatga tcgagttgag catctcaatg
    64741 aaccaggtgg gggaaacccg aatccccagt tcgggatctt tgagcgccgg aaccgcgatc
    64801 gccgccagcg cccgcatggc gttattgcgg accgcatcgg cggggtcctg cattgcatat
    64861 tgcaggtcgt ccacaacaag ccgctttttc tcggcgtagc cgatcacata ggccgcgacc
    64921 gagcgatgct catcgtcgcc ggactcgcga aggacatccc gcagaagctt tagattactc
    64981 ttggcgaact cttcaaactt cagttggagc gcacggcact ccggatctgc cagcagggag
    65041 tgtccacgcg tgaggtcgtc gcgggcgtcg ccgctgcgca cagcccgttc cagtgccgcc
    65101 aggaattgcc ggtatgtctc aacgacctga tccggcagcc tcacttccga attcggtgga
    65161 gtgcggaagt taaagtgtgg agcgcctttc tcctcgatcc cgacgaaaag gatcgccttg
    65221 ccgtccgcgc agcacactga ttcgaggcgc gcgcgcacca cgccggaaac ctgttccagg
    65281 cgctcctcga ctaccgattt cgagggagga aggggatccc cttcgcgtgc gcccagcgcc
    65341 ttcaggagcc tctcttcgga cactttacga acgccataga agtcgataat gccgatgcgc
    65401 gggatttgcg caaggctaag cgtttgcgcc agcaagagcg gcagtgcgag tttcacacag
    65461 atcagacgtg cgcggtcagg cgccggctac gcctgttttt gggcgaattc gccgatgacg
    65521 gggagaacga ccttggtttt cccctcgtaa gtcttccaga tcaggtagat ccagaggatc
    65581 acaaccgcta gactgaggag cggtagaaga acagcctgga cgaagaacgg cagaatggcg
    65641 ctgagcgcga tccacaggac aatccaggcg cagttcagga tgatcgactg gaacgcgtgg
    65701 aagcgaaccg tcttgttctg gttgtagggc gcgaggacca gaaagagaat ccccgtgatc
    65761 aacccgagca cataacagag tgcgccggct acattgtccg aaagaccagt cccggccgct
    65821 tgaggcgcag ctccactccc cgtgccgcca ctttcgccga ccgaagcgcc gcattgcggg
    65881 cagaacttcc cctcaaccgc cgaaccgcat tgagcacaga aagccatgaa tccccccggc
    65941 gactacttta ctgtagtctc agcctcttgg caatcgatga gatcttcaga ctaaactatc
    66001 ggaaaggaaa tatttttcga ttataagacc tgaaacggta aaatagacat agcccgcgcg
    66061 ttgctgcaag gcgagcccgt ttcctgaaaa cgggagatgg ccgcttgggc gttgcccggg
    66121 tcgaccgagt agtcgtttgc tccttccagc ccggaaatgc cgtcggttga ggcacacggc
    66181 gcaggaggtg ggtgatagcg cagagagccg tttgtccggt caggaaacgg cgcttttcct
    66241 gtgccttatt cgaacgtggc gggaacgagg ctgaatcctg ttaacacatt gattctatgg
    66301 atgatgcgat ttggttgacc tgtgccccat cgaagcggtg gtgcctacgg tgatgaggct
    66361 ctttttctgg cctcaaaagt gcagaatcat gcagtaatcc ccaaggactg attcaagatg
    66421 aacagaccca aagtgaccat tgacggtaat gaagctacgg cttataccgc ccaccagatc
    66481 aacgaagtga ttgccattta tccgatcacc ccttcctcga acatggggga gtgggcggat
    66541 gagtggtcgg caaaaggcaa gaagaacatc tggggcgttg tcccgactgt tgtcgagatg
    66601 cagagcgaag gcggagcgtc aggtgcgctc cacggcgccc tccagggggg atcgctggcg
    66661 actacattta ccgcctcgca gggcctgctg ctgatgatcc cgaacatgta caagattgca
    66721 ggcgagctca ctgcggcgac catccacgtt tcggcccgca ctctcgccac gcatgcgctc
    66781 tcgatttttg gcgaccacag cgacgtgatg gcctgccgcg ccactggttg ggcgatgctt
    66841 tgctccaact cggtgcagga ggcgatggac ttcgcgctga tcgcacaggc cgccagcctc
    66901 gaatcgcgcg tgcccttcct gcactatttc gacggcttcc gcacgtccca cgaagtggcc
    66961 aaggtcgaac aactccaggt ggaagacctg aaggccatga tggacgacga gttgatccgg
    67021 gcgcaccggg cgagggcgct ctcgcccgac cacccggttc ttcgcggctc tgcccagaat
    67081 cccgacgtct tcttccaggc ccgcgagacg gtcaacccgt actacatcgc cggcccgacg
    67141 attgtagaga acgtcatggc gaagttcgcc aaggtcgttg gccgccagta ccggctgttc
    67201 gactacgtag gcgctcccga cgccgaacgg gtcctggtgg tgatgggctc cggagcggag
    67261 gtagcgcatg agactgtcga gtacctaact gcccgcggcg aaaaggtcgg tgtgctgaag
    67321 gtgcgtctct tccggccctt ctcgatcgaa cacttcatcg cggcgctgcc gaagaccgtg
    67381 aagaatatcg ccgtgctgga ccgcacgaag gaaccgggcg ctgtcggcga gccgctctac
    67441 atggatgtgg taactgcgat cagcgaagcg ttcgcgcgcg gccaggcgcc gcttgcatcc
    67501 ttcccgcgga tcattggcgg acggtacggc ctgtcttcga aggagttcac gccggcgatg
    67561 gtgaaggccg ttttcgacga ggccggcaag gagaacccga agaaccattt cacggttggc
    67621 atcaacgacg acgtcacaca taccagcctg cagctcgatc ccgaattctc gaccgaagat
    67681 ccgtccaccg tccgcgcatt gttctatgga ctcggctccg acggcacggt gggcgcgaac
    67741 aagaattcga tcaagatcat cggtgaagac accgacaatt acgcgcaggg ctacttcgtg
    67801 tacgactcga agaaggccgg ggcgataacg atctcgcatt tgcgttttgg ccccaagccg
    67861 attcgttcga gctatctgat cagcaaggcg agtttcgtcg cctgccacca gttctccttc
    67921 ctcgagcggt tcgacatgct caaatccgct cagcccgggg ccgtcttcct gctgaacagc
    67981 ctgtatggtc ccgatgaagt gtgggaccac ctgccgaaga aagtgcagcg gcagatcatc
    68041 gacaagaagc tgaagttcta cgtgattgac gcctacgaag tggccaagaa gaccggcatg
    68101 ggcgtccgca tcaacacgat catgcagacc tgcttcttcg ccatcagcgg tgtgctgcct
    68161 cgtgaggatg ccatcgccgc aatcaagaag gcgatcgaaa agacgtacgg caaacgtggc
    68221 gaggctgtgg tgaagaagaa tttcgccgcg gtcgatgcag cgctcgatca tctgcacgaa
    68281 gtcaaggtcc ccggcgcggt caccagcgcc ttcgacatgc gcccggccgt gcccccgcaa
    68341 gcccccgggt tcgtccagaa gacgctggcc ggtatcatcg ccggcgaagg cgactcgctg
    68401 cccgtgagcg cgttcccggt ggacggcaca ttcccgacgt ccacggcgca gtgggagaag
    68461 cgcaatatcg cgcttgaaat cccagtctgg gacgaggctc tttgcatcca gtgcggcaag
    68521 tgcgtgttag tctgccccca cgccgtgatc cgtgccaagg tttacgacgg cggcgcgttg
    68581 aacggcgctc cggagacatt taaggccgta cccgcgcgtt ggaaggattt caaagatcag
    68641 aaatacacct tgcaggtagc tccggaagac tgcaccggct gcggcatctg cgtcgcggtc
    68701 tgcccggcca agagcaagac ggaggtgaag cacaaagcca tcaacatggc gccgcagccg
    68761 ccgatccgcg agacggagcg ggccaactgg gagtatttcc tgaacctccc cgagactgac
    68821 cggcgcgtgc tcagcctggc ccaggtgaag gacaaccagt tgctcaggcc gctgttcgag
    68881 ttctccggcg cctgtgcggg ctgcggcgag actccgtaca tcggactgat gacgcgactg
    68941 ttcggggacc gggctgtcgt cacgaacgcg accggctgct cgtcgattta cggcggcaac
    69001 ctgcccacca ccccttactg cgccaatgcg gagggacgcg gtccggcctg gtccaactcc
    69061 ttgtttgaag acaacgccga gttcggcatg ggcatccggc tcgccatcga taatcagacc
    69121 ggctacgcca agcagcttgt gagccgattg gccccggtga tcggagacga cctcgcgcag
    69181 gcgctgcttg gcgcggacca gtcgagcgaa gccggtatcc tcgcgcagcg agagcgggtg
    69241 aagctgctca aggagaagct gagcggcctc aagaccgccg aggcgcggga ccttgccgcc
    69301 gtcgccgaca cgcttgtcaa gaagtgcgtg tggattctcg gtggtgacgg ctgggcatac
    69361 gacatcggct acggagggct ggaccatgtg ctggcctccg gcgccaacgt aaacctgctg
    69421 gtactggaca ctgaggtcta ctcgaacacg ggcgggcaga tgtccaaggc cacgccgctc
    69481 ggtgcggtcg cgaagtttgc ctttggcggc aagccgaaac ccaagaagga cctgggcatg
    69541 atggcgatga cctacggaaa cgtttatgtc gcccgggtgg ccttcggttc gagcgacatg
    69601 cagacggtga aggcgttcct cgaggccgag gcctatgatg ggccgtcgct gatcatcgct
    69661 tacagccact gcatcgccca cggctacgac ttggtcaacg ggctggagca gcaaaaagcc
    69721 gccgtccagt ccggccactg gccgctgttc cgctacaacc cgaacctggt gggccaaggc
    69781 aagaatcccc ttcaactgga ttcgaaagcg ccgacgctcc cgctcgacaa gtacatctac
    69841 aacgagaccc gctacacgat gctggtccac agcaaaccgg aggcggcgag cgagttgctg
    69901 aagatggccc aggaagctgt ccagagccgt tggaagctgt acgagcactg ggcgagcatg
    69961 cccggcgacg gccaggtgca ggaagcaatc aaggaagccg tgaagcagac gccggctgag
    70021 gagtctaaaa atgcttgatc tgactaccaa ctacatgggg ctcgccctca agaacccgct
    70081 agtggtgtcc ccctcgcccc tgtgtgagca tattggaaac atcctgagga tggaggacgc
    70141 gggcgcttcg gcggttgtgc tgcactcgct gttcgaggag cagatcacgc tggagagcga
    70201 ggaactgaac gaggctcttt cggccggtac cgaaagtttc gccgagtcgc tgtcctactt
    70261 cccggacatg acgcggtaca acctcggccc cgacggctac ctggaacaca tccgcaaggc
    70321 gaaacaggcg gtggacatcc cgatcatcgc cagcctgaac ggggtttcga caggcggatg
    70381 gatcaaatac gcccaggaga tggaacaggc cggcgccgac gcactggaac tcaacatctt
    70441 ctatctggct aacgatccgg ccctgacaag cgagcaggtc gaagagatgt atgtcggcct
    70501 tgtctcccat gtcaaggcga gcgtgcggat cccggtcgcg gtcaagctgg ggccctattt
    70561 cagtgcgatg gccaacatgg gtaagcggct cgaccaggcc ggcgcggatg cgctggtact
    70621 gttcaaccgc ttctaccagc cggacttcga cctcgaagag cttgaggttg tcccgaactt
    70681 gcacctgagc acgtctcacg agttgctgct ccggctgcat tggatcgcaa cactctacgg
    70741 ccagatcaag gcggacatgg cgctcaccgg cggcgtgcat tccgcgcagg atgtattgaa
    70801 aacgatgatg gccggagcca gggtggcgat gatgacatcg gcgctcctcc gaaacggtat
    70861 caatcacctc gagaccgttc tcgcggacat gaaggcctgg atggaggagc atgagtacga
    70921 gtcggtgagc cagatgcagg gcagtatgag ccgccgtgcg gtggccgacc cctccgcgtt
    70981 cagccgcgcc aactacatga aggtacttag ctcttacacg atgaagacta tcgggaacta
    71041 gaatcgcgcc cgaggggcgg gttcctactc gcccctcttt cctaatccgc ccccgctcga
    71101 agcagttctc tcagcccgcc gacgctttct tcctggccat gatcggacgc agaaggaggt
    71161 tcaccacgac catgatcacc gatgccacgg gcacgaccag cagcgccgcc ttcagtttct
    71221 gatcgtcgcc gcccgcaatg ccgccgatga ccttcgagct caccgctaga cccacccatc
    71281 cacaggtcag cacgatgccc atcgccgtgg cggtcgcttt cgggaacgcg tcgcccgcca
    71341 tcgcgactgt ggtcgggaac accggcgcca tcgcgaggcc ggcgcagaag accgccagcc
    71401 atgccaccgt cggatcgccc gtctggagcg ccaggtaggt ggtgaccgcc atcagagccg
    71461 aggaagccag cgtgacggtg gccgccgcga cgttcaccag gattcgtgac acgacgagcc
    71521 ggccgatcaa cagcccgagc gcgaagccca gagagaggat tcgcaacgcc tggccttcag
    71581 ggactccctg tgcgatcagg tgcttcggaa gccagttcca gacgccgacc tcacaagcta
    71641 cgtacaagaa catgaagaac gaaagcaggt acagagcggg ctggccgagt accgttccga
    71701 tctctgagaa cttgaagccg cgctcgccgc tcggaggcgg catcactgtc acgctgttga
    71761 acagaagcgt cgccaccgta agcgccgcca ccaggtagca caacgcaatc gcgttgccgg
    71821 caaggaagtt tgcggcgatc aggggtgtcg ccagtccgcc caggccgaag aacaggttca
    71881 ggaggttcag cgtggttgcc cgccgctcag ggttgatgtc gctcgccagc gcgctgccgg
    71941 ccgtgacaag cgtggccccg ccgagtccga gtacgaacat ccaaatcatc acctggagcg
    72001 ggctgccgga gttcggcaac gcgaaaagcg cgccggcgat caggatcagg gacgcaacga
    72061 gcccggtttt cttgcctctg ttgtcgatga gcgggccgga aagcacggaa gcaacgatca
    72121 gtccgagggc ctgagcaaac gcaatgtcgc cgctttgctc cggagtgagg ccgaacttcg
    72181 tcgaaaggtt cggcagaagc gtcccgagca tcgcggcgat catgccgtag accagaatcg
    72241 ccagaatcgc cgctacaatg agccggctcg ttttcacggt tgccatccac atcctccaaa
    72301 aaagaggccg caggccggag caacggctcc aaactgcggc caacgctatt atcacacgct
    72361 tcccgcactc aacctgcgta cgacggccgg tgcgcagtta cgacgtccgg tagttcttcg
    72421 tcttctcgtc ccagtagacc ttcttgttgt tgcggtagga gatcgtggcg agatggccgc
    72481 cgatggcagc ctcctggccg acgctggcgg gcgcgatagc cttcgccttt ccgagcacag
    72541 ccaatacaaa attctttacg tgcgcctgca ccgccgtgtt gtcgttgttc gggatctcaa
    72601 ggtggatctc cttgcgggcg gcgatgttgg ccggcggctt gccgcggaac gactccggat
    72661 agaagtgcag gatctggcga tttagcacct ggatggtacc ttcgttgccc aggaagtcct
    72721 cgccataccc gaaatggtcg ttgccgaagt aacagctata gttgatgtgg aacttgtcgg
    72781 ggtactcgta gatggcgctc agggtgtcgg gcacctcgcg gtcgtcattg gcgtcgaccc
    72841 accggtaaac gccgcccgaa gccatgcagg aaagcggcac cgttttgccg cagacgaagt
    72901 tcgtgatatc ggtttggtgg accagcagat cggttgagat accgcccgag tagtcccaat
    72961 acagccgcca ttgatagtat cgattcttgt caaacggccg cttggcggct gggccgagaa
    73021 aacgctgcca atcggtgttt tgctcactgg cgtccggcgg aatggcatac cgccatgctg
    73081 gggcgtttga gagcgagttg cggtaccagt aggcgcgagt gtaatgaatg tcgccaatca
    73141 gcccctgggc aaccaattcc ttggccatga tgtataggga gttgcttcgg ttctgcgtgc
    73201 cgacctgcac cactttgccg gacttctcgg ccgctttcac gatgtcccag ccctcttcga
    73261 tcgtgtgagc gagcggtttc tccaggtaga tgtgcttgcc cgcgctcaaa gccgcgagag
    73321 ccatctcatg gtgcaagtgc tccggggtgg taatgaagac aatgtcgatg cccggctgct
    73381 ggagtagctc ccggtagtcc acatacgtct ttggcgcggc gccctctacc gtctgaacgc
    73441 ggtccttggc cctgttcatg tgtccctggt acgtgtcgca taccgccgtg acatgcacca
    73501 actgcggcac ggactcatac atcatgttca tcacgtagtt gccacggctg ccggtgccta
    73561 cccagccgac gttcagcttg ttgttcgcgc ctaatgcggg aagcacaacg ggaccggcgg
    73621 ccagcgccgc ccccgccgac cgcttcatga aacctctccg ggacagttct tcgggcataa
    73681 ttgcagtatt ccttcctgtg aatgttctaa gcattcagaa tagcaacgat ttcggagtcc
    73741 gtcttcctgt ttgctttgtg acgccgccgg cggaaaaaaa gtgggaaaag atgcggccag
    73801 gactaggggg ggatcggagg tcccggccgc gagcgttgac gtggggtaag tctccggacc
    73861 tgccttctag agccgtgatt cgttcaattc cgtgtttcga atgacgcgga attcgcggtg
    73921 gtgatcctgt ccggagcggc tgtcttcaag ggtgaccgtt aatgtcatct ccttacggtt
    73981 ccttaccacg gtgatcggaa aggtcttctg ggaacgggcg ctgcggatgg cgctcgagac
    74041 gtcccggggc gtcgttactt tcgtctcgct gaccctgatg ataatgtctc cggctttcaa
    74101 gccggccttt tccgccggcg aatccttgag aaccgacctg accagcacgc cttccttgac
    74161 gccgaagtag tctgccaact gcgcctcgag ggattccgcc tcaacgccta gcacactcgt
    74221 gcgccatgac atgtacgcct tcgggatgtc cggcatgcgg aactgcaact cgttcagcga
    74281 ctccatttct tcgccgagac gcgcccagtc cttgccggga ggcatcgcga acacccgcgc
    74341 ctgctgctgt ttgcggctcg cggtcgtcgc cgtgagcgtc tgggtcttgc cgtcccggct
    74401 gagcaacagc ctcacgttcc ggccgatggg cgtttcccgg accaatcgca cgaactgctc
    74461 ggtgcccacc accttctggc cgttgtactc gagcacaatg tccccggtct tcataccggc
    74521 cttggcggcc gggctgtcgg gaaccacctt ggtcacttcg acgccgcgct cttcgtccag
    74581 cttcaaagac cttgcccgat cggagtcgat ctcggtaacg ccgatgccca ggaaactccg
    74641 gctcgcggga tgcaggccgg ggaagaagtc accctgaacc tcctcaggag gcgcagaagg
    74701 atccggtggt tcaggctggt agccagcagc cagattgccg cccatcagga gcgtcgcaat
    74761 aaccacacag attgggaaga ctttcataaa tacctcaccc ttgcacccta cggccacacg
    74821 cagccgcgga cgctgttcag tcctgacggc gcaggtagat tgtgccgccc gtagccgaga
    74881 tccacaactg cggtccgccg ccgtttagga cgccctccgc aatcgcaggt ctggcctcac
    74941 gaaatcgtcc gagtctcacc tggatttcgg ggaagtcgga tacgattctg cccgacatcc
    75001 ctttgccttc gttatgagcc tgaacggtca ccgccagatt agacgggatg aataccgtaa
    75061 cgtcaccgcg gccggtggtc aaattcgaat cctcgaggcg ggcgttcgat ggaatctcag
    75121 cgagaatgtt accggacgcc gtggcggcga gcagggcgcc cgaaacgccc cgcagttgaa
    75181 tcgtgccacc cgccgatccc agtcgcacac cattcgcgga tgcgacccgg atgcagccgg
    75241 aagcgctttc cgctattacc ggtccgtctg cctcgatcac gtcgatcatc ccgccggacg
    75301 tatgcgcagt gaccgttgcc gccgcgcgcc gcaccctgat atttcctcca gcggtcgatg
    75361 cgtgcaccgg gccgcgagcc tcgccgatgt aaatttcccc gccggcagtt tcgaaaagcg
    75421 cctcgccgcc gacgcgctcc gcgtgaatcg tccctccgcc ggagaagcag cgcagaaatc
    75481 caccgacccg accaagccgg atttcaccac ctcccgttcg cacggtggcg ccccccccga
    75541 tgtcgtccat tcggatccgt cctccggcgg cgcgtacatc gaccgcacca tccaggcctg
    75601 tcgcctcgat ggaacccaga cgcgtctcga gcacgcaacg tgcgaggctc cgtggcaccc
    75661 gaagctcgag ctccgatgag gcagataaat cgaccgggta tgctacgaca aggcgggtca
    75721 aagcgccttc acggcggacg ttgaaagaaa tccgcttgag cagcgccgcg gcttgcgccg
    75781 catccctcac ccgaacccgt ttcttcaaac ggtagtcgat ggtctcctgg ccggcgccgc
    75841 gcacgatgag cggaccgtcc gcgcggacgc gcaactggcc ccctggcgac gattcgactg
    75901 tgccttcgca agtctgaacc cagtaagcgt cctcctgggc gattggactg gatgtctggc
    75961 cgaatgcagc ggcggcaaca accggaatca gccagtggac tcgcatcaga aggttcctac
    76021 ggaagcgttc atctcacgga gcgtcttttg acaccgctgg cgcacgtact cgttgctatc
    76081 ccgatacaga gcctcctgaa gaagccccgc catccctgct cccttgtgct gcaccaggag
    76141 atcgatcgcc tgcgtacgaa cgcccggatt ctcatccgtc agcaggacgt cggcaagcac
    76201 cttccggacc tccggttcgg cgctgaatga cttgaggcct tcaatggcct tcagccttac
    76261 accggcaact ggatcctgcc ggagcgcctg aagaagcact cgccggactt ccggggaatc
    76321 cgctcccgat ttcagtatgt ccatcgattc gacgcgcaac ccgggatcgg ccgcatcact
    76381 ggctgccgca agcaacaacc gctggatagc caaatcctcc aggctaccgg agaggatgcg
    76441 ctgccttgtc tcatccatca cgatgcgcac gcctccagaa gcatcgggtt gcacgtatcg
    76501 tacttgggtg gcagtcggac catgtccggc gttgtcggct tgactgacgg gagaaacgcc
    76561 cggcgcggtg agacgggcgg cgaggaaacc agccagaacc aatgcaagcg caccgatcgg
    76621 gcgcatgtac ggagcatgca agacttctcc tagccactcc caaagggcaa acgtccggtg
    76681 cggacgccgt tcctcggcca gccttgcccg gagctcctgc cggaattgcg ccagaagatt
    76741 tgcgggcggc tccgtcgcgt gcaggtccac tgcctcgtgc cagacctttt cgcgctccag
    76801 cgcgtgacgg catccttcac aggactccag atgctgctgg agagcttcct cctcatcgaa
    76861 gctaagctct ccgtaaagaa acaacgagag gcggcttctg gccaattcac aagtcatacg
    76921 aaatccccta gtgcttgccg cattttctgc gtcgcgcgga acaggcagtt cttggcggcc
    76981 tcctcgctag tacccaaaac ctcgccgatg gccttcaatc gcatgccctg gtagtgccgg
    77041 agttcgaaga ccatccgctc gcggggggta aaaccgcgca agacctcgcc gatgcgcctg
    77101 cccagttccc ggctcatcaa gtgacgttgc gggtctccgt ccacccgctc ttccgggacc
    77161 atctgcatga agtcgagagt gccggattct gtgtgggcca ccggcgactc ctcccgccgg
    77221 accttccgct tgcggaggtg gtccaggcag aggttggtca cgatccggta cagccacgtg
    77281 tgaaagctgc agtcgaagcg aaaggagtgt aagttcttgt aaacacgaag gaaagcgtcc
    77341 tggtagacgt ctcgggcgtc ctcgacgcta cgcagcagat tcatcgccag tctgagtaca
    77401 ctttgatcgt aggcgcgaac gagccgctcg aaggcatcct gatcgccttg ctgggcggcg
    77461 cggatcagag ccgcttcgtc cattgctgag gtcaggccga tgtcactcgt caatgcggct
    77521 tcacgttgga ccgtcaacac ccactttgac ggggctcatg gaaaaacggt aggaactccc
    77581 gccgctagcg cccgagcatc ctgccgccgt cagcctgcga gttccagttc gatctgcttg
    77641 ttgccgggat cgagtgcaac gatccggaag ctgcgaatct gcccggcccg caccgcttcc
    77701 cggcgaacgg ccgcatccgg cgtctttcct tgcttccatt ttgctgaaag cattgctgtt
    77761 agcgccgtca ggtcagcctt gctcgagccg gcttcggtcg actctgcagg cctttccggc
    77821 tcggctggaa tccggcaagt tgcatggaca ccctcgccga cctcgacttt ggccttgccg
    77881 ctacgaactt ccagcacgcg gccgctcacc gtgtctcccg acttgtgttc ggcgatgtac
    77941 tcgtccgccg tcgtcggctc cagttgtttc atgcccagcc gcaagcgtcg tttcgccttg
    78001 tcgatttcga gcaccaccgc ccggaccttc tgcccggtgt ttagcatctc cttcggatga
    78061 ttcagacgct tctcgcggct gatatcgccg atatggatca tgccttcgac gccttctcca
    78121 aggtcgacga aggcaccaaa ggccgccaga cttacgaccg gtccctccac caccgtgccc
    78181 gccgggtaac gcgtctcaat atcctgccaa ggatcgccca gcgcctgctt caggccgagc
    78241 gcgatccgtt tgtccacggt gttgacgcca agcaccacta cgtccacagc ctcacccgcc
    78301 ttcaccacgt cactcggctt gcggatcttc cttgaccagg acagctcgga gacgtggatc
    78361 aggccctcga cgccgggctc caactcaaca aacgctccaa actctgccac ccgcgatata
    78421 atgccgcgca cgcgctgtcc ctgtgagtac ttctccccga ccgccgtcca cgggtcggga
    78481 ctgagttgct ttacgcccag cgagatgcgg cggctttcgc ggtcgatctt caggatcttt
    78541 acccggatct gctcaccttc ggtcagcagg tcagagggct tgttcacccg accccatgac
    78601 atgtctgtga cgtgcagcaa cccatcgatg ccgccgatgt ccacgaacgc gccgaactgc
    78661 gtcaggctgc gaacggctcc ctcgacttcg gcgccttcct gaagctcgtc gaacaccttc
    78721 tgccgcgcgc gggcctcttc ttcttcaagc actacacggc ggtccacgac gacgtcttcg
    78781 tcgccgacat cgagcttgat gattcggcac tgcacttcct ggccgacgaa cttctccatg
    78841 tccgcctggt cccggatccc actcttcgag gcgggaagaa acgctcgcac acccacatcc
    78901 accgtcaacc cgcccttgac cacacccgtg acgacgccgc cgatcgtctt cttctcggcg
    78961 aacgcgcgct cgagcgatga ccagtctttt ggtctttcga cacgcacctt cgacagctcg
    79021 tagtacccgc cgccatcgcg gcccttaatg gtgacgataa ccgtgtcgcc gggttggata
    79081 ccggcgttgc cggcggcatc cacaaagtcg cccgcgggga ggacgccttc catcttcagg
    79141 ccgatgtcga cgaacacgga atccgcgttc acggcgacta cgcggccttg gcgagcctcg
    79201 gagcgcccgc cctcccggcg gtgcgactcc tcgaactgag acaggatttc tccaaacgtt
    79261 gaggtctcga gcgacgcggg ttggtcctca aggccaggca gagggggtgt tgggtgattc
    79321 attggcaaac gcggtagttc tccattctga cacaatcccg ggtaagatgg cagcgtggca
    79381 gccacgcctt ggatcagtct cctccggcgc gcggacgtct tctggctggt gctggtcgcg
    79441 gggctggcgg cggccagccc gacgcgctat ccgggagaga tccccctgct ctccgcgctc
    79501 gctattgttc aggtcatcga ggcgaaagtc ggattcttct ccacttcgcg cggcagcgtc
    79561 gccgccaatc tgctgaaact gctcctgggc tatttcctga taggctacac gggcggcatc
    79621 gagagcagct actacctggt cctcgtgctt ccagtcatct ccgccgccgc cacgctcggc
    79681 gtgatcggga cggtcgcatt cacactgctc gcctgcgcct cgtacctttc gttcctgctc
    79741 tatatcgact ggacacgatg gatcctcccg tccgaccagg ttcgcgaact cggcttgcgc
    79801 gccaccttcc tggcgatcgt cggtttcctc gcctatcgcg tagccgaggc aaagggcgaa
    79861 gaggcgcacc ggtatcaggt gacggcgcag cagcttgccg ctgccaaccg cagcctgaag
    79921 gaggcggaag cggccgtcag ccgttccgag cggctggccg cgctcgggca gctatcagcc
    79981 ggcctcgcgc acgaactgcg gaatccgctg gggacgattc gcgcttccgc cgagatgctc
    80041 cgaaagaaca ttcccgccga aaactcggtc gccaacgagc ttgcaggttt tatttcctcc
    80101 gaggtcgatc gaacgaattc gctggtcacc cggtttctgg agttcgccaa accactcgca
    80161 ctccgcctgc acaaagccga cctgacggag gtcctcgacc gggcagtgac gcgctttcag
    80221 cggagcccgg ccgccgtgcg ggtgtccatt tacaagaact attctcctga tatcccgccg
    80281 ttcccgctcg acgtcgagtt gatggaacgg gtggtcttca atctgctcgc caacgccgcc
    80341 gaagcctccc ctgcggatgg cgccgtgacg ttgaagaccc ggccggtcga cggaaccgtc
    80401 gagatttcag tcatcgaccg cggcgcgggt atcgacccga agcaccggga aagcatcttc
    80461 aaccctttct tcaccaccaa accggatgga gtcgggctcg ggctcgcaat cgtctcgaag
    80521 atcgtcgacc agcatggcgg taaaatgacg gtcgaaagcg aattaggcag aggcagcgtg
    80581 ttccgcgtct ttcttccgtt aggcggcact gcttgagaat tcatcgtgag taaacaccgg
    80641 attctggttg tagaggacga ggataagctt cggcgcgttg tcgagctcca gttgcagtcg
    80701 gcgggcttcg aggtggacca agcagcatca gccgaggacg ccctcaagct ctccgaccgc
    80761 gccgacctcg tcctgaccga cctgcgcctc cccggaatgg atgggctgga gttgctcgcc
    80821 gttctgcgcc ggcagaattc gcgaacgccc gtggtggtga tgaccgcctt tggaaccgtc
    80881 gaaggcgccg tggaagcaat gaaagcgggt gcgttcgact tcctgatgaa gcctttctcg
    80941 ctcgatcact tgatggccgt catccagaag gcgctcgagg tccgggcgct gcgggacgag
    81001 aacatcagcc ttcgggagga gcttggccgg cgctacacga tcgacaacat catcggccgc
    81061 agcaacgcga tgcaggaggt cttcgccacc gtcatgcgcg tcgcgccgac gcgcgccacc
    81121 gtgctgatcg ccggggaaag cggcgttggc aaggatctga tcgcccgcgc catccattac
    81181 cactcgcctc ggcgcgatcg tccgttcgtg aagatcaact gttcggcgct tcccgagaac
    81241 ctcatggaga gcgagctgtt cggcttcgag aagggcgcct tcaccggcgc ggtcgccgcc
    81301 aagccgggta aattcgaaca ggccgacacg ggcaccgcgt ttctcgacga gatcggggat
    81361 gtgcccgcct cggtacaggt gaaactcctg cgcgtgcttc aggaacgtga gttcgagcgc
    81421 ctcggcagca acaagacgcg ccatatcgac gtccgcatcg ttgccgccac caaccaggat
    81481 ctgcgtgcgg cgctcgagca gggaacattt cgcgaggacc tctattacag gctcaacgtc
    81541 gttccgatga acatcccacc tctgcgcgaa aggaaagagg atatcccgtt tctggccgag
    81601 cacttcgtca tgaagctaac acgggatgcc ggctcgcgcg tcgagtcgat taccgaagcg
    81661 gcggtccgca agttgatgga gtaccactgg cccggcaatg tgcgagaact ggagaatgtg
    81721 atcgagcgga gtctggtgct atgcacggga acaaagctgg atgccggaga catcaagctg
    81781 gacacgactc ctcgggcagt ggcgccgccg tccggacagt tcctccccga aggcctcacc
    81841 ctcgacgaac acgagcagac gatcatacgc gaggccctgc gccgctccgg cggcaacaag
    81901 agccaggcgg cgcgcctgct cggactgacg cgcaacgcct tgcgctaccg gctgtcacag
    81961 atgggactgg aaagcctgga cgcttcgtcg cagggctgaa atccgtctcc catcctgccg
    82021 cgcgaaccgg taaactcttc caacatgaac cgacgtgact tcctgtcgca caccggcgct
    82081 gccggcctgt ccgcacaagc tcaggcgatc gccgtcgagg gtccccgtta tgccggcaag
    82141 cccgccctca ggattaccga catccagcct ttcctggtcg gcctcggcgg tcgcaactac
    82201 tgcttcgtca aggtgctgac cgaccaggag atccacggat ttggcgaggc ttactcggtc
    82261 ggacctgatg aggccactgt cgctaccatc cgcgacttca aacactggct tgtgggcaag
    82321 gacccccgca atgtcgagta cctgtgggcc atgatgtacc agttcacccg ctttcccggc
    82381 ggcgccgtca tcaacgccgc catcagcggc atcgagcatg cgctttggga catcgctggt
    82441 aaggccgccg gtttgcccgt ctacatgctt ctcggcggca agtgccgcga gaaggttcgg
    82501 gtctatcagt cggcgggagg cgccaccccg gcggaactcg ccgcgagttc gcaacgcctg
    82561 atctcgaagt acggatacac ggcgctgaag atggcgccgt acccgccggg ctcaagcaaa
    82621 ctcccctaca acgccgtcac acgcgccgcc gccgaacggg cacaggcggc ccgcgaggct
    82681 gtcggacccg atgtcgatct tggatttgac gtccacgcca ccctcttcga accggtcagg
    82741 gcctttcaga ttgccgaggc ccttaagcct gcccggccat ttttcatcga agaaccgctc
    82801 cgtatggaga atgtcgacgc actcgcggag ctaaagcgga agatcgagat tccgctcgct
    82861 accggcgagt gcctttacac gaagtttgag ttccgcgaga tcctcgccag gcaggcggcg
    82921 gacatcgttc agcctgacgt ctgcctgacc ggagggatca tggaattaaa gaagattgct
    82981 gccatggccg aggcgcacta tgttgtggtg gccccgcaca accccatggg tccactcgcc
    83041 acgatggtca acgtccactt cgccgcctcc acgccgaatt tcctgatttt ggaataccac
    83101 cccgacgaca cgtcgccccg caaggacatg atcaaggatc cgatcctggt aaaggatggc
    83161 tacctgccca taccggacaa acccggctgg ggttacgaaa tgaacgagga ggccttcaaa
    83221 catcatccgc cgaaaccgtg gcatcgtggc ttcgcgttcc ggtccgacgg ctcaccggat
    83281 ttcatctgac ggcttgacga gctacaaccg cacaatcttc tccgactgga acgctctcat
    83341 cgcgttccgt gtgtccacga tcagccgcgc cttctcgagc agcttggcgt agtcgaacgc
    83401 cgagtggtcg gtgacgatta ggacacagtc ggcttcggcc gccccttcgt ccgccgcctt
    83461 gctacacagt tcgacaccgt ccttcttcaa caccggcaca tggggatccg agtacgtcaa
    83521 ctgcgccccc cgcttttcga gcagcaggat gatgtccagc gccggcgact cgcgcacgtc
    83581 gtccacgtcc cgcttgtacg cgacgcccat gatgtggata cgcgagccgc gcaacggctt
    83641 gctgtggtcg ttcagggcgt tctgcacttt gtcgaccacg aattgcggca tttgcccgtt
    83701 gatataaccg gccagttcaa tgaagcgtgc ctcgatcccc gcctgtttcg tcttccagga
    83761 aaggtagaac ggatcgattg gaatgcagtg gccgccgagc cccggtcccg gatagaacgg
    83821 catgaatccg aacggcttgg tagccgccgc gtcgatcacc tcccacacgt tgatgcccat
    83881 gcggtcgcac atcagcgcca tttcgttgac catggcgatg ttgatcatgc gaaacgtgtt
    83941 ttcgagcagc ttcaccatct cggcgacctg cgtcgagctg accggaacga ctttttcgag
    84001 cgcctgttcg taaaacatgg cgcccattcc ggtgcacgcc ttcgttaccc cgccgaccac
    84061 cttcggaatg ttcgaggtct ggtacatcgc gttgccagga tcgacgcgct ccggcgagaa
    84121 gcagaggaag aactcctcac cgacccgcat gccgggccgg gtgaacatcg gcaacagcaa
    84181 ctcatcggtg gttcctgggt aggtcgtcga ctccaggatc aacagcattc ccggatgcat
    84241 gtgcctggcg atctcctggc aagcggagac gatgtaactc atgtccgggt ccttagtttt
    84301 ccgcagtggc gtcggcacac agatattgat cgtgtcgaga ccggccaccg ccgcgaagtc
    84361 ggtggtcgcg cgaagctttc ccgccgccac cagtggcgca agcacggccc cgggtacgtc
    84421 ctgaacatac gaatcgcccg agttcagcct ctccgccttg gtcttgtcca ggtcaacgcc
    84481 ggtcacggcg aagccagcct tcgcaaactc gacagccagc ggcagtccga cgtatcccag
    84541 cccgacgacg cccactcttg ccgtccggtt ctttattttc tgttccagcg ccttcgccac
    84601 ggcgtttgct tccgttgcca tcgcttgcct ttctcagtcc aggtagtact cgccgccggc
    84661 gacgatctcg gccggccccg tgaggtaaac ggagccgtcc caccgtgcgt cgaggggacc
    84721 gccctccgtc agaatgcgca ccgggctctc caccagtccc cggagcgccg ccgcccatgc
    84781 cgcgccggtg gagccggtcc ccgaactcat cgtttcgcct gctccgcgct cgtagaaccg
    84841 aacctctatg gagtgccggt cgagcggccg tacgaacgac acatttgtgc gcctcgggaa
    84901 ctgtggatga cattcgatct cggcccccgt tttccgccag tcgaactcga agttctcgac
    84961 gaaaacggcg cactgcggat tgccgacatc caggatcgtc acctcaagag tgccggtgga
    85021 gagcggcagg ggaaaccgga gttcttctgg tttccaactc ggacggccca tatccatctc
    85081 gaacaagtac cgcagcctgt cccggttccc gacacgcagt tccttcagcc cggcgccggt
    85141 cagaatgcgc accgtctccg cacccagccc gctatcaaga agaaacgcgg ccgcgcaacg
    85201 ggtgccgttg ccggaaatct ccgcctcgct cccatcggcg ttgaacaagc ggataacggc
    85261 atggtggcct gactccgggc cggccggcgt cactagcatc cagccgtcgg cgccgatgcc
    85321 gctgtgccgg tcgcagatcg cctcggccac ggaggggaga ttctcgagcg gcgcctcgtt
    85381 ggtccacgta agcaggaaat cattccgggc gccgtgtgcc tttgtaaatg ggattctcat
    85441 atgcctgagg tgtctccgtg cagtggcagg gcgggacgta gccacgccgg cgccccggcg
    85501 cgccggtaaa tgtgaatcac cggcgccccc ttcaccacga tcgtcttgac gcacttgtaa
    85561 cgcgggccgt ggcgttgcgc cctgacgatc gcatccgaga ccttgtcgcc cgcgatggtc
    85621 aacgcccaat cttcccatag gaagaggtcc ggccttccgg ctgccgcctc ccaatgcggg
    85681 acgttgccgt tgtggaatcc ctcatggaga gggatgcccg cctccctcag cacgcccgcc
    85741 aggtcgccga acgagaagaa gattccttgg cccggacggt agttctcccg caggaacact
    85801 gcagcctccc tcgtccactt ccgccgcgcc tcggagttca cctgagactc tttccagcaa
    85861 acccacgcgt ccggacgcgg cgcgaacgcc catggagaaa ccgaagcggc ggcgatcccc
    85921 aatgccgccc agcctctcgc tcgcaccggc agcacggagg cgagcgctcc cgcgcacaag
    85981 gctaggagcg gcagagcctc cagcccatat cgagtgttgt agtacgtgtt cggccaaaga
    86041 tgagggacga aaatcggcgt agaccccgag tgcatactcc ataggatgaa cgcagccggc
    86101 agcgccggca gaaccagcgc ccagaaccgc cgtttccaca gcgccgcaat cgcgccggcc
    86161 atggccagcc atgcaagcgg acggcccgcg cacaacataa ctgcgtcgtg gaagtagagc
    86221 caagcctttc cccaatcgtg gtcgccggga tacttcgcca tcccacccac cagcgcccgt
    86281 tgatagatcg ccttcgccga gtacggcccg cgatagaact ccaggcaatc gccccagtac
    86341 caccagttgt gagccagcca ataaaggggg ccgagcgacg ccaccgcgcc gaacaccaga
    86401 gcccacgtca gcctccggcg cttcgccgcg agaaggaagt acgccgttac gaacgggatc
    86461 aggaaccacc cttcgtaacg ggtgagcgtg cccgccagcg cggcaatccc cgcgccaagc
    86521 accgctcccc ccgactgggt gtcgcggaac cgcacggtga agtacaggag cgccgcgagg
    86581 caggcaaaaa aaaccggctc cgtcatcggc gccgcctgga gatacagaag atttggattc
    86641 agtgcgaatg ctgccagcga tgccgccgct gccgcccggc tgtcgaacgc gcgccgcgct
    86701 gccgaataga ggaatgcccc tgccagaacg taacaaaccg ccacggggat cgctcccgcc
    86761 aggccgctgc gccaccaccc gtcatctctc gccagtggga tcatcagcgc gtgcggcagg
    86821 ggaagccaca ctgtaccgat ctgaaagtac gccggggtcc gcgaatcgag cacacggcgg
    86881 gcgatgttca ggtgcgcctc cgcatcgcca tagaacaaag tccagccttc acgggcgcac
    86941 cacagcaccg cggccgcgct cacggccgcc agtagaggca gtaccacgaa aggatcaaaa
    87001 cccggtaaac gctttgaatt cttggaagcg gccatcgatc tccgcgcgcg tcagggtacg
    87061 aaatcgctcg acaccgaaca tttcgcaaca gtagcttccc atgaccgaac cgtataccac
    87121 tgcgttgcgc aggtgcctgt cgtcaatcgc tcctttggac ggatcggcgc cctgttcggc
    87181 aaggtagccg atgaagccgc ctgcaaagca gtctcctgcg cccgtcggat cgaagacttg
    87241 ctccagcatg taacccggcg cagtgaagtg cccgtcgcgg cggaacagaa ccgcgccgta
    87301 ctctccccgc ttcactacca gggtgtgcgg gcccatgtca agaatggcgt gggctgcccg
    87361 gcgcaggttg tgctcgcccg aaagttggcg cgcctcgctg tcgttaatga gcaggaagtc
    87421 ccattcccgg atcgtttcca acaactcctc gcggtagtcc ttgatccagt agttcatcgt
    87481 gtcgccaccc gccagccgca gtccgttcat ttgctcgcgc acgctccgct gtagcgccgg
    87541 ctggatgttg ccaagcatca ggtacggctg tttcttgtag tgctccggca gtttcggctg
    87601 gaaatcggcg aacacgttga ggtcggtccg aagggtcgtc cggtcattca tgtccgccga
    87661 gtaaatgccc tgccaataaa agctcttgcc gggaacgcgt tcgaggcccg tcagatcgat
    87721 gccccggctg ctcaagaaat cttcatcctc gcgggtgaaa tcgtcaccca ccacggcaac
    87781 gatcttaacg tcagtgaaat agctggccgc caacgagata tacgtgcagg cgccgccaag
    87841 aatccggtcg acgcgtccgt gcggtgtctc aatgccgtca taggcgaccg agccaactac
    87901 aacaagcgac atttcttgat tgtagccgtg gcgcaggctt cacacaggcc ttccgtccgc
    87961 tcgcgttaca atgaaggatc ccgatcatgg tgcgctgggt tcgctttctt tcactagccg
    88021 tcatcgtcgc gggctatctc tggatgcact tccggatggg ccgctggctg gcggcgcttc
    88081 cgtctgtgcg tcgttctccg gcccggcggt tcggcctctg gcttttcgtg ggaaccatgg
    88141 gcgcgctgct ttgtctcggt cttcttctgg ggctctcacc tgtggcccgc gccgttcagc
    88201 cggccctgtg gcctgcctgg gtgcgggcgg cctcgttcac gtgggcgttg ctgtcgttcg
    88261 tatccggctg cgctgtctgg ctttggcggc atacaccgga cttcgatccg caacgccgcc
    88321 ggctcctgcg cgttgcggga ggggccgcgc tggctgcgcc agtggccgcg acgggattcg
    88381 ggatgttcgt cgagcgcggc aatctgcggg tgagcgaggt ggatatcccg ctccgtggct
    88441 tgccccggga ccttgacggg ctccggctgg cccagttgac cgacatccac atcgggccgt
    88501 tcctgagcga gaaggaattg gcacgcgctg tcgacatggc gaacgagtta cgcccgcatc
    88561 tcgctcttgt cacaggcgac ttcatcacct cgctcggtga tccgctcgat gcctgcctct
    88621 atcaactcgg ccgtctgaaa gcggaagccg gcgtgctggg ttgcctcggc aatcacgagg
    88681 gctatgcggg tgtcgagagt tacaccactc gccagagcgc tcgacgaggc atccgcatac
    88741 tacggcacgc gtcggtaagg ctgcgctttg gccgggcggc cctcaatctt gccggggtcg
    88801 actaccagtc atttttcgaa ccctatctgc gaggcgcggg ggcgatggtt cggaaggaca
    88861 gcctgaatgt gctgctctcc cacaacccgg acgtattccc ggtggcggcg cggcacggct
    88921 tcgatctgac gattgcggga cacacccacg gcgggcaggt caacttcgaa attttccgcc
    88981 agtcaatcaa cgtggctcgc ttctacaccc cgtacacgta tgggctgtac cggcacgggc
    89041 gggcggctgc ctatgtcaac cgcggcatcg gaacggtcgg tatcccggcg cggattggag
    89101 cgcctcccga aatcgcgctg attcgcctat gcgctacctg atcatcagcg atatccacgc
    89161 gaactgggag ggccttgaag ctgttgcggc cgatgccgaa gggcggtacg accagattct
    89221 ctgcagcggc gacctggtgg ggtactgcgc cgatcccaat tccgtgatcg actgggtgcg
    89281 tcgaaacgtg gcgctcgtcg tccgtggaaa tcacgacaaa gcttgctccg gcttcgagga
    89341 tctggagtgg ttcaacccgg ttgcacgcgc ggccgcgctc tggacccagg cggcacttac
    89401 gcccgggaat ctggagtacc tgcgcaactt gccgaaaggt cccctgcagg ttgaggattt
    89461 ccagatcctc cacggttcac cggccgacga ggacgagtac ctgctctcat cattcgatgt
    89521 ccatcaggct cagtcctatg tcgagcgtga tctgacgttc ttcggacata ctcacatgca
    89581 gggcggcttt ttttggcatc gcaacggcgt gcggaggatc aagcggactc ccccggacgt
    89641 ggcggagcac cgcgtggctc tcgatccgga ttccatctgt ctcgccaatc ccgggtcggt
    89701 tggccagccc cgtgatggcg atccgcgcgc cgcctacatg ctctactcgc cgggcgagag
    89761 agagctcgtc tatcgccgcg ttgcctatga cgtcgccgct gcccagcgaa agatccgcca
    89821 ggcgggactg ccggatcagc tcgctctccg gctggccctc ggaacctgaa cgcgatcaac
    89881 atcctcgcgc cggccgtaat ctcagcgtgt ctccccgccc tatccatccg cccgatcagc
    89941 gtagtgactc cgtacccggc gtgccgccgc ccgcccgacg gacgccgcca gctcgtcttc
    90001 gctcgcggcc cgaaccctct ccgcgctgcc gaaatcccgc agtagctttt gcacggtctt
    90061 gggtcctacc cctgccgcct cgtccaactc cgaaaccagc cgcttcgact cgcggcggct
    90121 tcggtgaaac gtgacggcga agcggtgcgc ttcgtcccgg atcgtctgga tcaggtgcaa
    90181 taccggcgag aagcggtcca aaatcaccgg ttcgtcttcc tgcccgagca cgtagatcca
    90241 ctcctcgcgc ttcgccaccg ccgccatcgg ttggttgatg agtccgagcg actccagcgc
    90301 ctctgccgcc gaatgcaact ggccaagccc gccgtcgatc agcaccaggc tcggcatcgg
    90361 ctttccctcg tcgtgcaggc gcgagtaccg ccgcgtcact acttcgcgca tcgacgcgaa
    90421 gtcgtcgttc ctgtcgaccg tgcgaatgat gaacttgcga tagtcggact tcttcatccg
    90481 gccgtcttcc cagaccacca tgcttgccac tgtgtcggtg ccctggatgt gggagatgtc
    90541 aaagcattcg atccggcgcg gcgcctccgg cagtgcgagg gcgtcttcca gcgcctcttg
    90601 gatcgccttt gtggacggct tcattatgcg gaagcgctgc tcgaagctgt gctttgcatt
    90661 tgtttgggcc agctccagca tcgccttctt ctggcctcgt tgcggtgtgt ggatttccgt
    90721 cttccggccg cgcttctcgg tcagcagttc ctcgagcacc tcctggtcct cgaactccgc
    90781 cggcacatgg atcagcgcag ggatgtgcgg ctgatcgaga tagtattgct tgagcagcgc
    90841 cg
//
LOCUS       DATIFQ010000544         5091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_572859, whole genome shotgun sequence.
ACCESSION   DATIFQ010000544 DATIFQ010000000
VERSION     DATIFQ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572859"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2662
                     /locus_tag="VLE22_27845"
     CDS             <1..2662
                     /locus_tag="VLE22_27845"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB18297.1"
                     /translation="VFNAVVDPITRTESVVKNNIWGGTLGNPIKKNKLFNFFSYERWK
                     ATQPSSNVSTVPTDLERAGDFSKTFTPQGDLRVIYDPFSTQFDPATSTVTRTPFTNNR
                     IPAQMIDPTGQKIINDLWKPNRTGDDLSGVNNFKIGYGWWIKYWNVSDRVDYNHSDKL
                     RMYWRYSRFETRLDNPNWGGTRAVRSDNGGLMDALNAAFDTIYMISPRTTLNLRMGAT
                     YAEDDYDSPWAQVGNEVWADLFPTKWYEPVTGALPGIYYPSFNFSGNGGASSGIGGWW
                     LVRERQYNGQANLTHTMGMHNLKFGFQVRHSYGKNGLPSPGGFNFNSVETGSSFLGYD
                     ATQSGSQFATALLGVVSSGNANISPMWDHHLNQFGFYVQDDIKLNRNVTLNLGLRYEI
                     ETAPKDVKDFMFTRYLDLTSPIPELQGVTLPAEVTAIAKSQPTFNGAWVFTDSEHPRT
                     FPTLHNILPRVGVAIRLNDKMSFRAGYARYATPIRSVYGEAWDIPKEGYSSSTSVLDP
                     LQGVPRTLISDPFPAGNQLILPTEKSLGRYTQLGSDGHWFNQDITAPKNDRFNLSLQR
                     QMPGNFLFDGTFFMHFGHNVQDNSMWGGSFNQPINLMDPNLAYTYKGAVDQAVPNPFY
                     QLLPPDKMPGPLRNEEMVSVSQLLRPYPQYKSLDQYYRKDISDHYYAMQLQLQRSMSQ
                     GVTLQVGYNYNHEIHGEFFNDIDLYNLKTTMIDRRRPRHSLRMAGTWELPFGKGKKYL
                     TNAPRALDLIVGGWSTAHWLMWQGGNLLTFSAAQVNGDPKSNVPDGLWFNPSVFQVNP
                     AYTPRTNPWYYDGLRGPRFWSLDSTLVKYFPITERARLEIRFEFYNMPNHFIPSDPDL
                     GIGSGTMGQSTWVYGGNYGREVQYTARIHF"
     gene            2808..2883
                     /locus_tag="VLE22_27850"
     tRNA            2808..2883
                     /locus_tag="VLE22_27850"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2841..2843,aa:Lys,seq:ctt)
     gene            complement(3136..3303)
                     /locus_tag="VLE22_27855"
     CDS             complement(3136..3303)
                     /locus_tag="VLE22_27855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18298.1"
                     /translation="MLLRLTNHEHDPMALRRNDERDRLGLDGRRRFEQEGNKSGWFAP
                     GPLAPKLSREK"
     gene            complement(3448..4620)
                     /locus_tag="VLE22_27860"
     CDS             complement(3448..4620)
                     /locus_tag="VLE22_27860"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSB18299.1"
                     /translation="MCEKRLLLLTAALWCVAGMATAATLQVGPARQFQTPCAAIAAAA
                     PGDLIEIDAGLYLGDVCAWYTDNLTIRGVNGRARLDAKNKNAQGKGIWVPYGRDTVIE
                     NIEFFGAKVPDKNGAGIRASGVNLTVRNCYFHDNEEGILESNIAGSNILIEYTEFDNN
                     GYRDGQSHNVYIGHVASLIFRYNYSHNSVTGHLVKTRAAVNYILYNRITGENGTGSYE
                     IDVPNGGTTYVIGNLIQQGPNTENSTLLTYLEEGFFPSNPGTDLYVVNNTFVNQRPAG
                     GTFVYVRPAGLTPPVLVNNIFYGPGTVTNRSDALATTNFIGDPLFVDVNKFDYHLTPG
                     SPAIDAGSSPGSANGYPLKPFDEYLDPACGEQRIPAGVIDIGAYEFGSAGKALSCR"
     gene            complement(4830..>5091)
                     /locus_tag="VLE22_27865"
     CDS             complement(4830..>5091)
                     /locus_tag="VLE22_27865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18300.1"
                     /translation="QMREVTYTFKVDGETLTGSMTGFRGQDLPISDGKISGDTLSFVV
                     KAEFGGNSMTWNHTGKISGDEIQMKREGSRGPAREFTLKRAK"
BASE COUNT         1022 a         1573 c         1457 g         1039 t
ORIGIN      
        1 cgtcttcaac gccgtggtgg atccgatcac ccgaaccgag agcgttgtga agaacaacat
       61 ctggggcggc accctcggca acccgatcaa gaagaacaag ctgttcaact ttttcagcta
      121 cgagcggtgg aaggccacac agccgtcctc gaacgtttcc acggttccga cggacctcga
      181 gcgtgccgga gacttctcca agacgttcac gcctcagggc gacttgaggg tgatttacga
      241 tccgttctcc acgcagttcg atcccgccac ctcgacggtg acccggacgc ccttcacaaa
      301 caacaggatc ccggctcaga tgatcgatcc cacaggccag aagatcatca acgacctgtg
      361 gaaacccaac cggacgggag acgatctgag cggcgtgaac aacttcaaaa ttgggtacgg
      421 ctggtggatc aagtactgga acgtctccga ccgcgtcgat tataaccaca gtgataagtt
      481 gcgcatgtac tggcgttact ccaggttcga gacgcgcctg gacaacccca actggggcgg
      541 cacgagggcg gtgcgctccg acaacggcgg cctgatggat gcgctgaacg ccgcatttga
      601 cacgatctac atgatctccc cgcggacaac gctgaacctg cgcatgggtg ccacttacgc
      661 ggaagacgat tacgattcgc cctgggcgca ggtcggcaat gaagtgtggg cggacctgtt
      721 cccgaccaag tggtacgaac cggtgactgg cgctttgccc ggaatctact acccgagctt
      781 caacttcagc ggcaacggcg gtgcaagctc cggcatcggc ggctggtggc tggttcgcga
      841 gcggcagtac aacggccagg ccaatttgac gcatacgatg ggcatgcaca acctcaagtt
      901 cggcttccag gtgcgtcact cgtatgggaa gaacggcttg ccgtcgccgg gagggttcaa
      961 cttcaactcg gtggagaccg gcagttcgtt cctcggctac gacgcgacgc agagcggcag
     1021 ccagttcgcg acggctctgc tgggcgtggt ctccagcggc aacgccaaca tcagcccgat
     1081 gtgggaccat cacctgaacc agttcgggtt ctatgtgcag gacgacatca agctcaaccg
     1141 caatgtcacg ctgaaccttg gcctgcggta tgaaatcgag accgcgccca aggacgtgaa
     1201 ggacttcatg tttacgcggt acctcgacct gacaagcccg ataccggaac ttcagggcgt
     1261 gaccttgccg gctgaagtga ccgctatcgc gaagagccaa cccacgttca acggcgcctg
     1321 ggtgttcacc gactctgaac atccgcggac cttcccgacg ctgcacaaca tcctgcccag
     1381 ggtgggcgtg gcgatacgcc tcaacgacaa gatgtccttc cgggccggct acgcgcgtta
     1441 cgcgacgccg atccggagcg tatatgggga ggcctgggac atcccgaagg aaggctactc
     1501 cagttccacc tccgtgctcg atcctctcca gggagtcccg cggaccctga tcagcgaccc
     1561 gttcccggcg ggtaaccagc tgatcctccc gacggaaaag agccttggcc ggtacaccca
     1621 gcttggcagt gacgggcact ggttcaacca ggacatcacg gcgcccaaga atgaccggtt
     1681 caacctctcg ctccagcgcc aaatgccggg caatttcctg ttcgacggaa cattcttcat
     1741 gcacttcggg cacaacgtgc aggataacag catgtgggga ggttctttca atcagccgat
     1801 caacctaatg gatccgaacc tcgcgtacac gtacaagggc gcggtggacc aggcggttcc
     1861 aaacccgttc taccagctcc ttccgccgga caagatgccc ggaccgctga gaaacgagga
     1921 gatggtctcc gtcagccagt tgctgcggcc ctatccgcag tacaagagcc tagaccaata
     1981 ctaccggaag gatatcagcg accactacta cgcgatgcag ctccagttgc agcgctcgat
     2041 gtcccaagga gtgacgcttc aggttggtta caactacaac catgagatcc acggcgagtt
     2101 ttttaacgac atcgacctgt acaatctcaa gaccacgatg atcgatcgcc ggaggccgcg
     2161 gcacagcctc cgcatggcgg gcacgtggga gttgccgttc ggaaaaggca agaagtacct
     2221 gaccaacgca cctcgcgctc tcgacctcat cgtcgggggt tggtcaacag cccactggct
     2281 gatgtggcag ggcggcaact tgttgacctt ctcggcggcg caagtcaacg gagatccgaa
     2341 gtcgaacgtc ccggacggcc tgtggttcaa cccgtcagtg tttcaagtga atccggcgta
     2401 cacgccgcga accaacccgt ggtattacga tggcctccgc gggccgcggt tctggagcct
     2461 ggattcgacc ttggtgaagt acttcccgat cacggagagg gcgaggctcg agatccggtt
     2521 cgagttctac aacatgccga accatttcat cccgagcgat ccggatctcg ggatcggaag
     2581 cggcaccatg ggccagagca cctgggtcta cggtggcaac tacggccgcg aagtacagta
     2641 cacggcccgt attcacttct agctgctgac ttcagcatcg aagccgcgga gggtgaccgc
     2701 tctccgcggc ttcttttttc ttgagcttgg agattcaggt ttcagcccct tatgcgcacc
     2761 cggagtatgg tataaaaaga gattgcgggg ccgcaccagc ccgccgtggg cgcgtagctc
     2821 aattggcaga gcaactgact cttaatcagt aggttgaagg ttcgattcct tccgcgctca
     2881 ccacgcaaat cgacgctcgg ctctacgcca gaaacggggg caagcccccg atcccaaaca
     2941 aaatcaactg gtacgttgga ccacagcgga aacgtgtatc tctccgtcgt cgggcaggtc
     3001 cggatggtgc tgcttaaggt ggacctggaa gcgtggaacc ccttcccatc ggggtgttcc
     3061 acccatatac ttgacccgtg aggtagcttg atgctccaca cgtttctctg ctcggttgtc
     3121 ttgtttacgt cggctctact tctctctgga gagcttcggc gccagcgggc cgggagcgaa
     3181 ccagcccgac ttattccctt cctgctcgaa gcgtcgccgt ccgtcaagcc cgagcctatc
     3241 cctctcatcg ttcctccgga gggccatcgg atcgtgctcg tggttcgtaa ggcgaagcag
     3301 caagctccgc gtgctccgta cctgggatgc ggtcagccgt gccttacacc tcacgcacgc
     3361 cgcagccctt cggctcaatg agaagtccgc ccgctcgagt tgttaggcag gcttgcgttt
     3421 ggcctgtcgc cgtgttctcc ggcgtcccta tcggcaagaa agggccttgc ctgcgcttcc
     3481 gaactcgtat gcgccgatgt cgatcacacc tgctggaatc cgctgctcac cacacgccgg
     3541 gtccagatat tcatcaaagg gctttagcgg atatccattg gccgagcctg gggaactccc
     3601 cgcatcgatt gcgggcgagc ccggcgtcag atgatagtcg aacttgttca cgtctacgaa
     3661 cagcgggtcg ccgatgaagt tggttgtggc gagggcatcc gaacggttgg tgacagtgcc
     3721 cggcccatag aagatgttgt tcaccaggac cggcggtgtc agtcccgcgg gacgcacgta
     3781 cacgaacgtc ccacctgcgg gacgttgatt cacaaaagtg ttgttcacaa cgtagaggtc
     3841 cgttccggga ttcgacggga agaacccctc ctccagatac gtcagcagcg tgctgttttc
     3901 tgtattaggt ccctgctgaa tgaggttgcc gatgacgtag gtggtccccc cgttcggcac
     3961 gtctatttca tagcttcccg tgccattctc gccggtgatg cggttgtaga ggatgtagtt
     4021 caccgccgcc cgcgtcttca caaggtggcc cgtaacgctg ttgtgagaat agttgtagcg
     4081 gaaaatcagg gaagctacat gaccgatgta cacgttatga gactggccgt cccgatagcc
     4141 gttgttgtcg aattccgtgt actcgatcag gatgttgctg ccggcgatgt tgctttccag
     4201 aattccctct tcattgtcgt ggaagtagca gttgcgaaca gtcagattca cgcccgaagc
     4261 gcgaatgccg gcgccattct tgtccggcac cttggcgccg aaaaactcga tattctcgat
     4321 caccgtgtct ctaccgtaag ggacccaaat ccctttcccc tgggcattct tgtttttcgc
     4381 gtcaaggcgc gcccgcccgt tgactccgcg gatcgtcagg ttgtcggtat accacgcgca
     4441 gacatcgccg agatagagcc cggcgtcaat ttcgatgagg tctcccggcg ccgccgcggc
     4501 tatggctgcg caaggcgtct ggaactgcct ggccggtccg acttgtagcg tcgctgcggt
     4561 tgccatccca gcgacacacc acagggcggc cgtcaagagc aagagtcttt tttcacacat
     4621 gcctcacctc cgatagcggg ccgctcgtac agaacccagc cttctgctaa tgtgcgctcc
     4681 ctagcgggag aagtcaacgg tcgctcccac gggttcttcg catcgccagc ctgcgtttcc
     4741 aggccgtttc gcgacgggct ggctccgcgc gaggacacca ctcccctcac tcttccgagg
     4801 tccaatgcgt gttcgggaga tcccgagtac tacttcgccc gtttcagcgt gaattcccgc
     4861 gccgggccgc ggcttccttc acgcttcatc tggatctcgt ctccggagat cttgcccgtg
     4921 tggttccagg tcatggagtt cccgccgaat tcggccttca cgacgaagga cagcgtgtca
     4981 ccactgatct tgccgtcgga gatcggaaga tcctgaccgc gaaagcccgt catgctgccg
     5041 gtgagagtct ctccgtccac cttgaatgtg taggtcacct cgcgcatctg g
//
LOCUS       DATIFQ010000545         7825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_1530729, whole genome shotgun sequence.
ACCESSION   DATIFQ010000545 DATIFQ010000000
VERSION     DATIFQ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530729"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VLE22_27870"
     CDS             complement(<1..478)
                     /locus_tag="VLE22_27870"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HSB18301.1"
                     /translation="MVMGIEHNTFICWSGEPSRHIARALRQWLPVVVQKSRPWMSEED
                     IGKGSSHFSEITQSLRQTRVGIICLTPQNLHSTWIHFEAGAIFNAIEGQHGVCTYLIG
                     GIQPKDLFPPLNQFQATVSTKEDTRRLVKTVHKTLGSTVEDETLEMAFEANWPRMQA"
     gene            522..647
                     /locus_tag="VLE22_27875"
     CDS             522..647
                     /locus_tag="VLE22_27875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18302.1"
                     /translation="MPRVSSNRARMEPSKFGLKGAAVERTETLEPASVKVVEVGR"
     gene            699..2072
                     /locus_tag="VLE22_27880"
     CDS             699..2072
                     /locus_tag="VLE22_27880"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSB18303.1"
                     /translation="MTSSRRSFLEMLVGLTSRINPPNVVLIVADDLGSGDLSSYGCRD
                     IRTPAIDSIGRQGVRFTQFYGTPECTPTRCALLTGRYQQRVGGLDCAIGVGNNGRYDE
                     AVWLQRRGELGLPTSELTLPRILKQRGYDTACFGKWHLGYPEKFRPNRHGFDEYFGIL
                     GGGADYFTHEEFNEGAGQSYLYHNVRKVKRDGYLTDLFAEAALDWLRRRSKRPFFLYL
                     PFTAPHTPIQDRDEYDPNTGTAPTRQGHRPTYAKMVERMDGHIGGVLKQLDAMGAAEN
                     TLVIFLSDNGGDANGDNRPLRGRKSSVWEGGIRVPCLVRWPGVLPAGKVISQLGMAMD
                     LLPTIVSATRTPAPSGRKFDGMDLLPVLTGERQPFPRLVFWRYKRGSAVRKTVRQGDM
                     KYVFDSGQEELHDLARDEREENNLLVERSDVVRDLKAKLTAWERDVSAERLWEFTAGS
                     PAVQTDP"
     gene            2146..3777
                     /locus_tag="VLE22_27885"
     CDS             2146..3777
                     /locus_tag="VLE22_27885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18304.1"
                     /translation="MAMALAILPTCHAQALRITAVQNEATRDSRLSPGCNAIVFVPDE
                     IQQCCSVGGKWAELNYHGSFTGLGNFIGVRIPVELPPGPTTISRSLSSEPVPITLSEF
                     APGIYSLDGTGAGFGYFRRQPFSSFAPEAHYLPTGLFRLDRNYPDLVIQDTVNRAVPG
                     EVLIAYAVGLGPTDPLISTGTKLDPFSTISSAPLVNPLRLMIDGRQAEVVWAGLFYAG
                     GLVGRMYDVGGLYNVRFRVPADLAEGNHEVALQIGGQTSNSVILPVGKRAPVITGLVN
                     AASFAYEGWSAPGSIMTLYGENIEGPDDLSGFPATTLQGISVIFNGVRAPLFHLIPSH
                     NQINLVTPAELQEDKDCWPDWLDCVTVQLSSPLGVSPRYYLATGAVHPGMFVIGDPWR
                     PARRNVAALFGNTAWRVMPRFQARALNIPDDCAGVAAGALCGRPARPGDVIQIYCTGL
                     GRATPGGDPAGAPLPTGSLAPSDGNPLYMTVKKPEVTIGGLSADVQFSGLTPGLAGLY
                     QVNVRIPQDAPSGDDVTVVMRQTYYSDSATLAIEK"
     gene            complement(3857..4291)
                     /locus_tag="VLE22_27890"
     CDS             complement(3857..4291)
                     /locus_tag="VLE22_27890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HSB18305.1"
                     /translation="MEMELIARFFENDRYAKENGIRIVEIRPGFARTEMTVEPRHLNA
                     VGILQGGALFTLADLAFAAASNSHGVVAVACQADVTWFKAVESGKLTATAEEIARTRK
                     LSTCVVRITGQHDELVALFKGVAYIKGSPLELGIPGGAGSGW"
     gene            4343..5467
                     /locus_tag="VLE22_27895"
     CDS             4343..5467
                     /locus_tag="VLE22_27895"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HSB18306.1"
                     /translation="MATEVSGSSEVAAAAAPLTKSSTGRLVSLDAFRGFVMLLLVSEG
                     LGFSVLRNHPGWEWLAAQVDHVPWEGCVFWDLIQPAFTFMVGMAMPFAFAKRKAEGAT
                     DLEVFKHVAYRALMLIVLSNIYSNWGPATSHLQLQLINVLSQIAFGYLICFLIMRLRF
                     RSQVVAGAAMLAGFWALFVLFPGPEGPWSKTGNIGALIDLNVLGYNYSGYYTTINFIG
                     NAVTILFGCWAGMLVRTDKSHGYKLKVLLSCAAVGLALGLALQPLNPMVKRLWTCSFT
                     FFSAGWVLLMLAIFYWIVEVKQVKRWTFPLIVVGMNSLFIYSLGQIGIKGWLTRGLGN
                     FTKNFGVFGDLGMILQHTLVMVCLWYACYWLYQRKIFFKI"
     gene            complement(5495..>7825)
                     /locus_tag="VLE22_27900"
     CDS             complement(5495..>7825)
                     /locus_tag="VLE22_27900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18307.1"
                     /translation="DFAIVDPLTNIPFQDNIIPPERISAVSKKIQDTLFPLPNFGPPG
                     LEAANYRQQFPGTTGFTRFNQFDIRGDYNFSSRDVVFARVSWRRMPLDYTDLVPTIGK
                     VTQRRYGHSAVASWNHTFSPSVVNEFRTGVTYHRNSYYLDVIGSDLIQQWGIRGTTVT
                     GINDAPIVRIDPVTYVDYDDYDDSYYNNPSATFQWIDNLSWTQGRHFVKFGVDVIRDR
                     LNETFITSLVYGGYNFTGVWSGFGYADFLLGIPQTSELAVPLPARNLRATTAGLYIQD
                     QFKVNRKLTLNYGLRWELMRPFSHTKGAIYSFNPQTGALVIPDEGAAQVNPYFPANIR
                     VETASEAGYPANSLLDSHNGYFQPRFGFAYKPFVAGNTVIRGGYGIYGNLVYRPMART
                     MGGGPFSGSATFINDIVDGAPLFQFPEPFLPSAIGAAGTQNVQGKNPHMKLPYTQQWN
                     LTVEQQVREVGFRVSYVGSRSVSLIYNRNLNLLAPSTTPFSASRRPYPQYNQVIWSDN
                     GGSEFYSGLEVSATKRQGKNLTFGAGWTWARDLTDTQDSGGGGSSYSGQVIQNQFDRR
                     AERANNGLVLRHRAFGYAIYLLPFGRGQRFLATAHPVVDAVLGGWQTSWSVNLQSGQY
                     FTPSFSGFDPSNTRTFGGRPDRIGNGNLPTGDRTIERWFDASAFKVPGCPDDNPVCQE
                     PANVGRFGNSGLNVLEGPGVANLDFALAKSFHFTEKSRLQLMVNMVNALNHPNFAVPR
                     ANISSRGTVGTISSMARVLNGLPAVRQVDLGLRLEF"
BASE COUNT         1503 a         2260 c         2410 g         1652 t
ORIGIN      
        1 cggcctgcat tctgggccag ttggcttcga atgccatctc aagagtctca tcctccacgg
       61 tagagcccaa cgtcttatgg acagtcttga caagccttcg cgtatcttcc ttcgtggaga
      121 ctgtggcctg gaattgattg agcggtggaa aaagatcttt aggctgaatc ccgccaatca
      181 gatacgtgca gactccatgt tggccttcaa tagcgttgaa aattgcgcct gcttcaaaat
      241 gaatccaagt tgagtgaagg ttttgcggcg tgagacatat tatgccgact cgtgtttgtc
      301 gcagagactg tgtgatctct gagaagtggg agctgccctt tccgatatcc tcttcggaca
      361 tccagggtct gctcttctgg acaacgactg gtaaccattg gcgaagtgcc cttgcaatgt
      421 gcctgcttgg ttcgccagac cagcaaatga acgtgttgtg ttcgatgccc ataaccatcc
      481 tcgcttactt tgactagtct agaatattgc ggacgactcg catgcctcgc gtgtcctcaa
      541 atcgcgcgcg catggagccg tcgaagttcg gcctgaaggg cgcggcggta gagcgaacgg
      601 agacgctgga accggcatcg gtgaaggtgg tggaggtggg gaggtaggat gggcaactca
      661 taggcaccgg ttctgtgcct gcgggaggtt ctgcactgat gacgtcttct cgacggtcgt
      721 tcctcgaaat gcttgtcggc ctgacgagcc gcatcaatcc gcccaacgtc gtcctgatcg
      781 tggccgacga tctaggctcg ggcgatctca gctcgtacgg ctgccgcgac atccgcacgc
      841 cggcgatcga ttccatcggc cggcagggtg tccggttcac gcagttctat ggcacaccgg
      901 aatgcacgcc gacccgctgc gcgttactca cgggacgcta ccagcagcgg gttggaggct
      961 tggactgcgc catcggagtc ggcaacaacg gccgctatga cgaggccgtc tggctgcaaa
     1021 ggcgcgggga gcttggcctg cctacatcag aactcacctt gccacggatt ctgaagcagc
     1081 gtggctatga cacggcctgt ttcggcaagt ggcacctggg gtatccggag aaattccggc
     1141 cgaaccggca cggattcgac gagtacttcg gcatcctggg cggcggcgca gattacttca
     1201 cgcatgagga gttcaacgaa ggcgccggac agtcctacct gtatcacaac gtccggaagg
     1261 tgaagcgcga cgggtacctg acggacttat tcgccgaggc ggcgctggac tggcttaggc
     1321 gccgttcgaa gcggccgttc ttcctttatc tgccatttac cgcgccgcac acgccaatcc
     1381 aggaccgtga cgaatatgac ccgaatacgg gaacggctcc cacgcgccaa ggtcacaggc
     1441 ccacgtacgc caagatggtg gagcggatgg acgggcacat cggcggcgtc ctcaaacagt
     1501 tggacgccat gggggcggcg gaaaacacgc tggtcatctt ccttagtgac aacggaggcg
     1561 acgcaaacgg ggacaaccgt cctctgcgcg gcagaaagag ttccgtgtgg gaagggggca
     1621 tccgtgttcc ctgcctcgta cgctggcccg gcgtgctgcc cgccggaaaa gtgatctcgc
     1681 agcttggcat ggccatggac cttctgccca cgattgtctc cgctacccgg acaccggcgc
     1741 cgagtggaag gaagtttgac gggatggact tgctgccagt gctgacggga gaaaggcagc
     1801 cttttccacg cctcgtattc tggaggtaca agcgagggag cgccgttcga aaaacggtgc
     1861 ggcaaggcga tatgaagtac gttttcgact ccggtcaaga agagctccat gacctggcgc
     1921 gcgacgagcg cgaggaaaat aacctgctgg tggaacggtc cgatgtggtt cgtgatctca
     1981 aggccaagtt gaccgcgtgg gagcgggatg tctcggccga gcggctgtgg gagttcacag
     2041 ccgggtcgcc tgcggtacaa acggaccctt gactctggtt tataccggga gcacactcag
     2101 ggcagcgcgc cactgatgca acgcaatact ttcacgatct cggctatggc aatggctctt
     2161 gctattttgc cgacctgtca tgcgcaagcc ttgcgtatta ccgctgtgca aaacgaggca
     2221 actcgggaca gccggttaag tccgggatgc aacgcgatcg tctttgtccc cgacgagata
     2281 cagcaatgct gttcggtcgg cggcaagtgg gccgaactca attaccacgg ctcctttacg
     2341 ggcttgggga acttcatcgg cgtgcgaatc ccggtggaac tgccgccagg gcctaccacg
     2401 atttcgcgca gtctcagttc cgaacctgtt cccatcacgc tttccgagtt tgcgccgggc
     2461 atttacagtc tggatggtac cggcgcaggc ttcggctact ttagaaggca accattttcg
     2521 tcttttgctc ccgaagcgca ttacctcccg accggtctgt ttcggctgga tcgaaactat
     2581 ccggacctgg tgattcagga cacggtgaac cgcgcagtgc cgggagaggt cttgatcgcg
     2641 tatgcggtcg gcttgggtcc gacagatccc ctcatttcca ccggcacaaa gcttgatccc
     2701 ttttccacta tctcctccgc gcccctggtc aacccgcttc gtctgatgat tgatggacgg
     2761 caggcggagg tagtctgggc agggctcttc tacgccggag gcctagtggg ccgaatgtat
     2821 gacgttggcg gcctttacaa cgtgagattc cgggtaccag ccgatctggc cgaagggaac
     2881 cacgaggttg cgttgcagat tggcggccag accagcaact ctgtgatcct gccggtgggc
     2941 aagcgggcgc cggtcatcac agggctcgtc aatgcggcca gcttcgccta tgagggctgg
     3001 tcggcgccag ggtcgatcat gactctttac ggcgaaaaca tcgaagggcc cgacgatctc
     3061 tcgggatttc ccgccacaac ccttcagggc atctcagtca tcttcaatgg cgtgcgggcg
     3121 ccgttgttcc acctgatccc atcacataac cagatcaatc ttgttacgcc cgccgagttg
     3181 caggaggaca aggactgttg gccggattgg ctggattgtg tcaccgtgca actgagcagc
     3241 ccacttggag tcagcccaag atactatctc gccacgggcg ccgttcaccc tggcatgttc
     3301 gtgattggcg atccttggcg gcccgcccgg cggaatgtcg ccgcgctatt tgggaacacc
     3361 gcatggagag tcatgccgcg gttccaagct cgggcgttga acatacccga tgactgcgcg
     3421 ggggtagctg ccggagccct ttgcggccgg cccgctcgcc ccggcgatgt gatccagatc
     3481 tactgcaccg gtctgggccg ggccactcct gggggcgatc cggcgggcgc tccgcttccg
     3541 accgggtccc tcgctccgtc ggacggcaac ccgctctaca tgaccgtgaa aaagcctgag
     3601 gtcacaatcg gcggcttgtc cgcggatgtc caattctccg gtctcacacc aggtcttgcc
     3661 ggtctgtacc aagttaacgt tcgcatccca caagatgcgc cctccggtga cgatgtcacc
     3721 gttgtaatgc gccagacata ttactctgat tcggccacgc tggcgatcga gaagtgaggg
     3781 acgacggggg aaggcaccgc tgttgacacg caaacgacgt tgggacagcg gagccagcca
     3841 gcgttgcgac gaggattcac cagccggagc cggcgccacc ggggatgccc agctcaagcg
     3901 gcgatccttt gatataggcg acgcctttga aaagagcgac cagctcgtca tgctggccgg
     3961 taatcctgac aacgcaagtc gagagcttgc gggtgcgggc gatttcctcc gcggtggcgg
     4021 tcaattttcc agactcgacg gccttgaacc atgtgacgtc cgcctggcag gcgacggcga
     4081 cgactccgtg tgaattcgag gcggccgcga acgcgagatc ggcgagagtg aagagcgcgc
     4141 ccccttggag gattccgacg gcgttgagat ggcgtggctc caccgtcatc tccgtgcggg
     4201 caaacccggg gcggatctcc acgatgcgga tgccgttctc tttcgcgtag cgatcgtttt
     4261 caaagaatct ggcaatgagt tccatttcca ttcgggatgt taccacctct ctctgtggta
     4321 agcttcagcg aaaggacaga ccatggccac agaggtttca ggttcgagtg aagtagccgc
     4381 ggcggccgct ccgcttacaa agagctcaac cgggcgactc gtcagtttgg acgccttccg
     4441 gggttttgtc atgctgctgc tggtctcgga aggcctcggc ttttcggtgc tgcggaacca
     4501 tccaggctgg gaatggctcg ctgcgcaggt cgaccatgtg ccgtgggagg gctgtgtgtt
     4561 ctgggacctc atccagccgg cgttcacctt catggtcggc atggcgatgc cgttcgcgtt
     4621 cgccaagcgg aaggccgagg gtgcaacgga cctcgaagtg ttcaagcacg tggcgtaccg
     4681 tgcgctgatg ctgatcgtcc tcagtaatat ctactcgaac tggggaccag ccacctcaca
     4741 cttgcagttg cagttgatca acgtgctgtc acaaatcgcc ttcggctacc tgatctgctt
     4801 cctgattatg cggctgcgct tccggtctca ggtggtagcc ggggcggcca tgttggccgg
     4861 cttctgggcg ttgttcgtat tgttccctgg tccggagggt ccgtggtcaa agaccggaaa
     4921 catcggtgcg ttgatcgacc tcaacgtgct cggttataac tattccgggt actacacaac
     4981 catcaacttc atcgggaacg ccgtcaccat cctgttcgga tgctgggccg gcatgctggt
     5041 gcggaccgac aagtcgcacg gttataagtt gaaagtgctg ttgtcctgcg ccgccgtcgg
     5101 gctggcgctc ggccttgcgc tccagccgct caatccgatg gtcaagcgcc tctggacgtg
     5161 ctcgttcacg ttcttcagcg ccggctgggt gttgctgatg ctggccatct tttactggat
     5221 cgtcgaggtg aaacaggtga agaggtggac attcccgctg atcgtggtgg ggatgaactc
     5281 gcttttcatt tattcgctcg ggcagatcgg gattaaagga tggctgacgc gagggctcgg
     5341 gaatttcacg aagaacttcg gcgttttcgg ggacctcggc atgatcctac agcacaccct
     5401 tgtgatggtg tgcttgtggt acgcgtgcta ttggctctac cagcgcaaga tcttcttcaa
     5461 gatctgaaca cagcctaaag gctgtgccgc cacttcaaaa ttcgaggcgc aggccgaggt
     5521 cgacctgacg gactgccggc agcccattca gcacacgagc catggaactg attgttccca
     5581 ctgtgcccct cgaactgatg ttggctcggg gtacggcgaa gttcggatgg ttcaatgcgt
     5641 tgaccatgtt gaccatgagc tggagacggc tcttctcggt gaagtgaaaa cttttcgcta
     5701 gcgcgaagtc aaggttggcc acgccggggc cttccagtac gttgagtccc gagttgccaa
     5761 agcgtcccac gttggcgggc tcctggcaca ccggattgtc atccggacaa cccggcacct
     5821 taaaggctga ggcgtcaaac catcgctcga tcgtacggtc gccggtggga aggtttccgt
     5881 tgccgatgcg atccggccgc ccgccgaacg tgcgtgtgtt ggaaggatcg aaccccgaga
     5941 aactcggagt gaagtactgc cccgactgca ggttgacact ccaactggtc tgccatccgc
     6001 caagtactgc gtccacgacc ggatgagcag tggccaggaa ccgctgtccc cgcccgaagg
     6061 gcagcaggta gatggcgtag ccaaaggcgc ggtgccggag aacgagtccg ttgttggctc
     6121 gctccgcccg gcggtcgaac tggttctgga tcacctggcc tgagtaggag gagccgccgc
     6181 cgccgctatc ctgcgtgtcg gtcagatcgc gcgcccacgt ccaaccggcg ccaaacgtga
     6241 gattcttccc ttgcctctta gtcgctgaga cttccagccc gctgtagaac tcactgcctc
     6301 cgttgtccga ccagatcacc tggttgtact gcgggtaagg cctgcggctg gccgagaacg
     6361 gagtggtgct tggggccagt aagttcaggt tgcggttgta aatcaggctt acactccggc
     6421 tcccgacata ggaaacccgg aaaccgactt cgcggacctg ctgctccacg gtcaggttcc
     6481 actgctgcgt gtaggggagc ttcatgtgcg ggttcttacc ctggacgttc tgagtaccgg
     6541 ccgcaccgat cgcggagggc aggaagggct ccggaaactg gaaaagaggg gctccgtcca
     6601 cgatgtcgtt gatgaacgtc gcgctaccgg agaaggggcc gcctcccatg gtccgtgcca
     6661 tcggcctgta aacgaggttt ccgtagatcc cgtacccgcc ccgaatgacc gtgtttccag
     6721 caacaaacgg cttgtaagcg aagccaaaac gcggctggaa gtatccattg tgagagtcga
     6781 gcagcgagtt tgccgggtag cccgcctccg aggcggtttc cacccggatg ttcgccggaa
     6841 agtaagggtt gacctgggcg gctccttcgt ccgggatgac caaggctccg gtctgcggat
     6901 taaagctgta aatcgctccc ttcgtgtggc tgaacggcct catcagctcc catcgaagcc
     6961 cgtagttcag ggtcagtttc cgattgacct tgaactggtc ctgaatatac aaacctgccg
     7021 ttgtggcacg gaggttgcgc gccgggagcg gaacggccaa ttcggaggtc tgcggaatcc
     7081 caagcaggaa gtccgcgtac ccgaatccag accagactcc cgtaaagttg taaccaccgt
     7141 agaccaacga cgtaatgaag gtctcgttca ggcgatcgcg gatcacatcc acgccaaact
     7201 tcacgaaatg tcttccttgg gtccagctca gattgtcgat ccattgaaac gtcgcgcttg
     7261 ggttgttgta gtacgagtca tcgtagtcgt cataatcgac ataggtgact ggatcgatcc
     7321 gcacgatggg cgcgtcgttt attccagtca ctgttgtccc acggatgccc cactgctgga
     7381 tcaggtcgct cccgatgacg tccaggtagt agctgttgcg gtggtaggtg acgcccgtgc
     7441 ggaactcatt gaccaccgag ggggagaacg tgtggttcca cgacgccacc gcgctgtggc
     7501 cgtaacgcct ctgcgtgact ttgccgatcg tgggcacaag atccgtgtag tcgaggggca
     7561 tgcgccgcca gctcacccgc gcgaagacca cgtcgcgact actgaagttg tagtcgccac
     7621 ggatgtcgaa ctgattgaac cgcgtgaagc cggttgtgcc aggaaactgc tgtcggtagt
     7681 tcgccgcctc caggccggga gggccaaaat tcggcaatgg gaacagcgtg tcctgaatct
     7741 tcttcgaaac cgcgctgatg cgctcgggcg gaatgatgtt gtcctggaac gggatgttgg
     7801 taagggggtc gacgatcgca aagtc
//
LOCUS       DATIFQ010000546        63545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2487607, whole genome shotgun sequence.
ACCESSION   DATIFQ010000546 DATIFQ010000000
VERSION     DATIFQ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 63545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63545
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2487607"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1243)
                     /locus_tag="VLE22_27905"
     CDS             complement(77..1243)
                     /locus_tag="VLE22_27905"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HSB18308.1"
                     /translation="MLQSRREILVQLAGVGLARAAARQQQLETKDDEVVASATADQTP
                     RVGVVLSSFDGGSDHDGTKLTGLARPKPVDATLNASELDAMFRKAVELSGVRGQDIES
                     IVEPSDWVVIKPDIAVCHDREGRIVPGSITDPRLVSSLIAWLVEKRLGERITIAEGPS
                     WRPETPVEVWTSDWNGAFGGFTYRKVVEEFSKKHAGVKFDVVDLNADKALELPIPGRP
                     SSSRNKEGVFFIPSTIQQCDKLITIAPLKTHARLLAALSLGSYFGILPETKYGRTKEQ
                     VFQLGEPHEILVDLYGYHASDYSILGGGWGLEGDGPYGPDARGVHHNVVIAGASALAV
                     DTVGASVIGIDPARMKHLELANRKGYGVAEIDLIWTRGNEVEQSRRPFRLPKGA"
     gene            complement(1834..3165)
                     /locus_tag="VLE22_27910"
     CDS             complement(1834..3165)
                     /locus_tag="VLE22_27910"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSB18309.1"
                     /translation="MERILVIDDDPGFRNLLETILVGEGYGVENAGSVAEAVRAGGRR
                     NYDLVVADMKLPDGDGLAVLRWWMENMPETPVIMITAFGTIASAVEAMKLGAVDYLGK
                     PLSSPDELRLLVRKALDQRQLAGNYALLREEEADRFSCGNLVADDPRMERVLELTRRV
                     APTDTTVLISGESGTGKELIARCIHKNSPRAEKIFVAVNCAALSPSLIESELFGHEKG
                     SFTGAAGQHLGRFERAHAGTLFLDEIGELDGNLQAKLLRVLQEKTFERVGGTREIAVD
                     VRVIAATNRNLRQSVADGKFREDLYYRLNTFPIEVPPLRERPSDIPKLARFFLGRAAR
                     NLGKPHLVLTPEAENVLLAYGWPGNVRELENTMERVAILCDDVIEPDDLPVTTTGPAR
                     PVLFKDIERQAIEDALRTNDGNRTKTARQLGISLRTLQYRLKEYCIAQTKD"
     gene            complement(3176..4411)
                     /locus_tag="VLE22_27915"
     CDS             complement(3176..4411)
                     /locus_tag="VLE22_27915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSB18310.1"
                     /translation="MSRRTQILSHLLLPVVIVALPLVLLLSSVRTFQELDEQKVVYLR
                     TRVAMLAARLENVPISETGTLVFEQLGKDEPSLIGLELIERGKAGDERRLQPIWNGQE
                     LFRAGFTGDSQEPVYRAYVPFHSDTGLRIARIDLDGRAADFLVAHARHNVIVASLGAI
                     VLVMVSSYGIWAARRTAQFKIRQLELEHLAHMGKMAAVLAHEIRNPLGTIKGFAQLAG
                     EKADAPLRQLLEPILGETERLEGLVHDLLLYGRPPAPSVRIAKWEETLGPLRAHARQI
                     IGTRDIRFVPDDPKLEWNTDPHLLQEALLNLVRNAADAIGDVAGGEVRLEIRSHRPQG
                     LILSVIDNGPGLSEIAQSRLFEPFFTTKAFGTGLGLAITRRLAQSLGGELSMNPGHPH
                     GTEAILHFPGLQAKEFEAS"
     gene            4504..4917
                     /locus_tag="VLE22_27920"
     CDS             4504..4917
                     /locus_tag="VLE22_27920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18311.1"
                     /translation="MRSVQFIVLTGLVVAGVALGQSSESQAPIADNPKVEVKGKIEKI
                     RIARGQGMPSLDLNTGDRTVSVILGSMRYLVEQDFNPKAGEEVVVSGYKVNDGIVAVT
                     VTLAATGRLLRLRDDGGRPLWIGGRRGAPMHRRAK"
     gene            5190..5522
                     /locus_tag="VLE22_27925"
     CDS             5190..5522
                     /locus_tag="VLE22_27925"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HSB18312.1"
                     /translation="MSGYYHQEDLPRFSEMGKNAPDLWKKFAEWYGAVFAEGALTARE
                     KSLIALAVAHAVQCPYCIDAYTQDCLEKGSNVEQMTEAVHVASAIRGGASLIHGLQMR
                     NAAEKVSM"
     gene            5519..6553
                     /gene="arsS"
                     /locus_tag="VLE22_27930"
     CDS             5519..6553
                     /gene="arsS"
                     /locus_tag="VLE22_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255296106.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HSB18313.1"
                     /translation="MSLVSIGHPLASAGEQLRVLSEPRRQLKPFEQSLAESGLLPLTA
                     TGITVLQINVGKLCNQTCRHCHVDAGPDRRETMARETAEHCIRVLAQTDIPTLDITGG
                     APELNANFRWIVERAKTLGRHVLDRCNLTILTVPSEADLAGFLAAREVEIIASLPYYL
                     EDETDRQRGEGVFAKSIEALRSLNALGYGQEGSGLRLNLVYNPVGAFLPPAQAAIEAD
                     FRRELLRRHGIVFNSLYTITNMPISRFLEFLLRTGNYDRYMEKLVSAYNPAAAAGVMC
                     RSMLSVGWDGTLYDCDFNQMLELRVDHGAPAHIRDFDPGALARRRIVTGQHCYGCTAG
                     AGSSCGGATA"
     gene            6750..8504
                     /locus_tag="VLE22_27935"
     CDS             6750..8504
                     /locus_tag="VLE22_27935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18314.1"
                     /translation="MSTQTVEPKPSPVMGLVNIDSSIEDVIRQRLAEERARLEREDAA
                     EKHKVHHFKRPAERSFTADQRGHTTLLFGGLTWKHEKLIHGALEGLGYISEAIPTPNV
                     SAFQTGKEFGNNGQCNPTYFTVGNLVQFLQSLEERGLTKQEIIDRYVFFTAGACGPCR
                     FGMYESEYRLALRNSGFDGFRVLLFQQSGGLSQSDAEAGLEMNIDFFLGILNAMNCGD
                     VLNEVAYAIRPFETSQGDTDAVLDRIMDYMHEVFRKKQPWTVGDNLAKYLGGFKDSTE
                     YIGKFVNQLKGDEYTASLERCRDLFNEIEVDRLRVKPIVKITGEFWAQTTEGDGNFNM
                     FRFLEREGAQVVVEPIGTWIMYMIHQVVQKHRDLKGLSEGAVLPPLWRLDKRAKIEWS
                     FRNKVAKLRLAEAIFKREYHRVVDALGGIAHHLADQYELQRVGHPFYNSRAGGGEGHL
                     EVAKNIYYSNKDLCHMVLSVKPFGCMPSTQSDGAQAAVVSQYRDMIFLPIETSGEGEI
                     NAHSRVQMALGEAKNKAKREFQEVLDRKGLTLEECRAYVDAHPEMKRPMYRVPHTKGV
                     VGTAANFVHHVAERMGRA"
     gene            8560..12129
                     /locus_tag="VLE22_27940"
     CDS             8560..12129
                     /locus_tag="VLE22_27940"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HSB18315.1"
                     /translation="MDTRFLIGLDVGSTTVKAIVIDAATDEILWRDYQRHDTKQPEKV
                     LEFLTRFEAEIDGFEQQASRIFVTGSGGSGVAKHIGAKFVQEVNAVSLAVEKLYPECG
                     SVIELGGQDAKIIIFKEDPETGRKKKLPSMNDKCAGGTGAVIDKINAKLRIPADELCE
                     MGYRGLKLHPVAGKCGVFAETDINGLQKAGVPPDELMASLFEAIVMQNLSVLTRGNTL
                     RPVVLLLGGPNTYIKGMRDCWKHNIPKIWEERNHPLPEGVPPEDLIRTPDNAQYFAAI
                     GAVEFGKTEDEGVGQYLGWNKLEWYVTVGREEEKAKRGGKGGLAKSPDELESFRGKYR
                     HKKFVPVTFQPDEVVEGFIGIDGGSTSTKAVLFSKDKPRRILAKAYQLSKGNPIEDTM
                     EIFARLEEQITGQGASLKVLGVGTTGYAKDILKDVIGADAAIVETVAHTQAGLHFYDD
                     VDVICDVGGQDIKIIILKNGRVKDFKLNTQCSAGNGYFLQSTAQGFGYPVEQFADVAF
                     GATGFPQFGYGCAVFMQSDIVDFQRQGWKPEEIMAGLCNVLPKNIWLYVSQIPNLSAI
                     GRRFLLQGGTQYNLAAVKSQVDFIESRFRGKEVPADVIVHEHCGEAGAIGAALEAGRL
                     WDHGRQSTFIGLKAVQEIRYQTTRSEATRCYFCKNKCLRTFIDVQTSVLNENGYRPPK
                     TKVPLADGSQRLIIATCEKGTVEDISEMRGIKGNLDKMKKANPNFVEIAARGVFRVPD
                     APIVADAPPKFQITAAQKKRAELMKRRASLRIGMPRVLNMYSVAPVFTGYFASLGVKA
                     ENMIFSEYTSEQLYKEGGKRGSIDPCFPSKLGIPHVHNLLYTQHAKKPLDLIFFPMID
                     CLPSDLACTQANRSCPTVAATPEAVKAAFTKEGDLFAEKGVRYLDTFVNLSKPDIFER
                     QMYEQFQDILGLSPEENRRAIAEGFKALDQFNNVTLRGAAREVLDQLEREQRLGVVVL
                     GRPYHNDPGVNHEILEEFQKLGYPVFTQDSLPIDDDMVWRLFGDEVRAGDVPHPMAIT
                     DVWKNAYSENTSRKVWAAKYVARHPSLVALELSSFKCGHDAPIYTVVEEIVEHSGTPY
                     FCFKDIDENKPTGSIKIRVETIGYFLKRYREDMIEAATAKDSIDARLAEYEKKLRLQL
                     LKEKLAALERQEEYVGGAMPQIHVSVGALAASRPQEPAHISGD"
     gene            12340..14622
                     /locus_tag="VLE22_27945"
     CDS             12340..14622
                     /locus_tag="VLE22_27945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSB18316.1"
                     /translation="MVHSRSGAIIHANNAACRILAVTLDQMLGKTSMDPDWQAVREDG
                     SPFPGEEHPAMVTLRTGQSLQGVVMGLYVGNPPAFRWISIDSAPINEPGTGELTEVMT
                     TFVDITGRKHDQEILRRSQQRFRDLVETTSDWVWEVDENLRYTYASPKVRDILGYEPE
                     EVLGRLPMDLMAPDEAERLSAMIADTIAKQAPFVALENTNVHRDGHLVVLETNGIPFF
                     DAQGTFRGYRGIDREVSERKRAEQELRRERDFSSAIVDTVGSLVVVLDRVGRIVRFNR
                     ACEQCTGYSAEEVTGKPFWDLFLTSEEYEGIRDAFLSIMSMSLVPDRPEFPSRRVNYW
                     LTRSGERRLIEWSNTVITGRDGSVEYVIGTGIDITDQRQLEEQFRQAQKMEAVGNLAG
                     GVAHDFNNLLTVINGYSQLVLDQLGDDSPLSKEVLEIRNAGERATTLVRQLLAFSRKQ
                     MAQPRVLDLNAVVAGVSTMLRRLIGENVELVTDLDPALPPVKADPGQVEQIVMNLAVN
                     AVDAMPLGGKLTVETRTADLDGAYAARHLGVQPGRYALLAVSDTGHGIDSEIRPHLFE
                     PFFTTKEVGRGTGLGLSTVYGIVKQNDGHVWAYSEQGIGATFKVYLPAAEGPVAPVSP
                     SLAAAEPESGTETILLVEDEPALRAMTKEVLRRHGYSVLEASNAAEALRVADEKGCRI
                     DLLLTDVVMPGMNGRELASRLAERCNGIKVLFVSGYTDSAVIRHGLLSASAEFLEKPF
                     PPSVLLRRIRQLLDAPGASD"
     gene            complement(14665..15423)
                     /locus_tag="VLE22_27950"
     CDS             complement(14665..15423)
                     /locus_tag="VLE22_27950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSB18317.1"
                     /translation="MNSAIEISHLRKEYDLVVAVADLSLKVPAGSFYGFLGPNGSGKS
                     TTIGCLTGLLDPTSGEMRVLGESTRPYPVSLKGRIGVMPENLGLFEALYAHEFLAFHA
                     RIYGLDEATTRVRVTERLEALELSGEGRKPLAQFSAGMRKRVAFAAAVNLFLDEPFES
                     IDPAGVALMKQWLRQLTAQGRTVFMTTHVLETVERLCDRVAIIRSPGDLVWEGDITAL
                     ARDMPIAVDGGEFRTLEGLFLHLTGERYATLRWL"
     gene            complement(15420..16904)
                     /locus_tag="VLE22_27955"
     CDS             complement(15420..16904)
                     /locus_tag="VLE22_27955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18318.1"
                     /translation="MIGYLLACFVAAILLLGGFGAAMASIRLGKAELVARVVLAGFSG
                     IAIVAALVLGIGVNPAFCDAVLRRYPLSAGDRFLTRRITAFLDPLWMILLALFMGLAA
                     GFHAVGVGSIWLAVLAALLLVIVTYLFAQLLAAMIGWITATPAGPAILAVLVVILVFA
                     VSAAPAIGANRSLSAALLPVVKVTPAFCAAGVMAGGSAIGLLLLLVWCAAMAAAIAGV
                     ERLPVPSQTVAAARAEWDGPVDRLVAHFGSSAPLVAKTLKYYWRTKRARFSLLINAPA
                     FPLMLVIMRPDRHAPLLHFFIGVGLMAIIGFSVTFAMAINSFGFDGSGFRRYFLLPLA
                     PTAVVRAVSLIPLLLGALTLPVGFAVCVLLARVPIDARMATMLAANGVGGLCFFQALA
                     LWTSMLTPSRTDGEVRFGNDYSLAANLVGSGGIVVSLVGSQFLGVAAGARALQFWWVA
                     PFFMVAAVAFYALSLCMAPAVLVSRRERILAVVEREQQGGRLIT"
     gene            complement(17198..17977)
                     /locus_tag="VLE22_27960"
     CDS             complement(17198..17977)
                     /locus_tag="VLE22_27960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HSB18319.1"
                     /translation="MKILFIGDIFASAGRRMVAQHLPRIIAAEKIDLTIANVENAAGG
                     FGITPPVADELFSYGIQVMTSGNHIWDKREIYDYLPRQAKLLRPANYPDDLPGSGSVV
                     VKATNGVQCAVMNFQGRTHMPPTACPFRKADELLAALDSSVKVKIVDFHAEMTSEKMA
                     MGWYLNGRVTGVFGTHTHIPTADTRILSGGTAYQTDLGMTGPYDSIIGVEKEIILRRF
                     LTSLPIRMEAAKTGAELHSVIIEADEATGKALSVRRYSTHE"
     gene            complement(18234..19898)
                     /locus_tag="VLE22_27965"
     CDS             complement(18234..19898)
                     /locus_tag="VLE22_27965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18320.1"
                     /translation="MKYILLVFTGLLTPGISCAQPVVDSVLHSASYSLPGTANWGIPR
                     GAIFTVFGRDLGPSGFKQVSAFPLGTQMDGITVQVKIGNTTIDAVPISRASVPLNGPA
                     TQISAIMPSAAPTGDGQLTVSYNGKTSSPIAVRVIENGFGMYTLNSGGSGPAVITDTN
                     YQVNLINQAAKPGQLLFLWGTGLGAVSYDEFAGPPRIEDLPYDVRVFVGGREAKVSYK
                     GRSGCCAGLDQVLFEVPSGAEGCFVPVAVMVRGSWTHFATMSIASSGKACSDPGGMAA
                     ADVERTMAAGEAKFGAINLGRVYSKIGTAEGSVDQASASFFRRSALEMLAGLGGVQVS
                     SVPGACMVFTIRAQDEVQWFSSDPAPARTLDAGPFVNVTGPQGSKQISLVSKGIYSST
                     LGGSQPDGQPLPDFLVPGDYKLDNGAGGADIGPFEARVTIPPAFAWTNRDGISDVDRG
                     QDLTLTWNGGDPAKQAVTIVGASLSGNIVGSFVCTERAATGSFTVPKSILRAIPSGSS
                     PVAHLPSGLLAVGVQPSPELTRFSAAGLDAANIQYTIQHIKGVTFR"
     gene            complement(19910..24160)
                     /locus_tag="VLE22_27970"
     CDS             complement(19910..24160)
                     /locus_tag="VLE22_27970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HSB18321.1"
                     /translation="MACLLRFVAPALLLPALFLPALCFGSRANAGFGNLPLSFEANRG
                     QFDPRVSYAARGEGFILFLTGGEAVFSLSGAGSTANVRMRFPGSPGPCRLDPLDPLPG
                     RSHYLTGRGPRDWKTGIPNYGRVACRGIYPGVTLVYYGNQRRVEYDLVVGPGRNPKDV
                     QVEFSGAESLRLDGGDLVLHTSAGALRQHAPLAFQDGPKGRRNIPVRYALRADHRVAF
                     EIGAHDPSLPIRIDPILSYSTFLGGDGAVTGGHDYGRAIAVDLSGNVYVAGSTQSTQF
                     PVTDGAIARTNQGSTDGFVTKFDPSGATRIYSTYLGGNSIDEVRGIAVDPSGNAYVTG
                     ATSSNRFPTTTGALQTSPKGGGDCFVAKLNQSGTALVYSTLLGGNSIDTGAAIGIDSA
                     GNAFVTGQTSSTDFPVTPGAFQKTLRSVPDAFFARINSSGTALLYATFLGGGYIDDAT
                     SLAVDQAGNAVVGGTTSSTDFPVTAGALRKTAQADEGFAVRFPAGSESPAYSTLIGGI
                     WRDAVYGVALDVAGNAFLVGSTGSTDFPVVNSIEPPPFAGGLLDPVRYLREILREWRF
                     LSLSGGQDAFVLRLNAEGTQILRGVFLGGGGTDTARSVWVDPSGRILVVAGETDSRDL
                     PVTRGARQSSFGGGAADGFVATLNFADFQLRYLTYLGGASEDRIYSAAGTPASEAWVT
                     GVTISADFPVTLGAWGGSLTRGGFDSFASRLDPLGSTLAFSSFLGGGGSSDAEAGNSI
                     SVDATGNAYVTGTTISTDFPVNEGALQNTPGGNSNTDVFVTKIDPAGKNIVYSTYFGG
                     SDKETGTGIAVDAAGSAYVVGVKGYGTLPCPAERSRGSGMAFVVKLSPMGSDVAYCYS
                     LGGNGYETSAAVAVDGSGSAYVTGATASANFPVTPGALQAVFKGQRDAFITKVDPNGT
                     ALLYSTFLGGSGFDDPSSIAIDSSGNVYVAGMTTSQDFPATAGAYQTRFGGGGWDTFV
                     VKLNAGGASLGYATYLGGDGNDEVYGIAGDPAGNTYLAGQTTSANFPTTEGSFTVAGR
                     SFVTKLNAAGSAIVFSNRLAEGNDEAGRAVAVDSSGNVVVAGRTTSAGLRVSEDAFQV
                     ALRGQSDGFLMKLDPAGKSLLYGTFLGGSGPDTAASVALDSAGNAYLTGQTGSADFPA
                     TAESFQPGLRGPRNAFVAKVDFSQTSPIERPVITKVVNFGSGGTAVAPGCIIEITGQR
                     LAKQTAEAGKPIEIRATPLPVQLGGTTVAVPGRVLPLFSVSADKIVAQLPYSVAIDSQ
                     TFLTVAVDGVSSRSFGVTPRGMAPGILAVYKQDGTLNTPTNLLHAGDTMIAYLTGGGR
                     TNPPVSSGVPAPSTQVEVIEKPQGIILSKPATPQQGTSGELKSFTLVPGLVGVAQAAI
                     LIPKHTDVGEGNKELDLTLTWNDVQSNRFRFYVLGSPD"
     gene            complement(24315..25619)
                     /locus_tag="VLE22_27975"
     CDS             complement(24315..25619)
                     /locus_tag="VLE22_27975"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB18322.1"
                     /translation="MEERNRRELLGMNVLAAALQAAGAQRDARPIRVGIVGSGDRGSY
                     HMDLLLGTDMVEVPAICDINQDYLYRAKRWVEEAGKPSPTLYGNGKTDFLRLCERTDL
                     DLVLCATSWEWHAPVCIAAMKAGKHAATEVPAALTLDECWEMVETSEKTGKHCVMLEQ
                     ANYSQHGLTVLEMAQKGLFGQLFCATGGYVHDLRLVKFDPEREPWRLQHSVDRNGNLY
                     PTHPIGPMAWWLDINRGDKFEYIVSMSSKAVCLNEYASLYYGDRHPYAGMKMKQGDVN
                     TTLMMTASGKIAVLYFDTNTPHPQTAELRLEGTKGHFSGNIQKVYIEGRSPEAHQWEP
                     LENYRADFEHPLWRNLDEKKYKRARGHGGGATAPVMWARLLRAIQRGTEPDMNVYDAV
                     TWSVISPLSEHSVARGSAPVDFPDFTRGKWKNTPPIKLTGEA"
     gene            complement(25636..26970)
                     /locus_tag="VLE22_27980"
     CDS             complement(25636..26970)
                     /locus_tag="VLE22_27980"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB18323.1"
                     /translation="MNQPIDRRDLLKAGLGVAAAGVLAPAASAQPKETIRVGFVGVGG
                     RGTNLMRILLKLPGVKMNALCDINEANLARAQKFVVDAGQPKPEGYSRGETDFLRLCG
                     RDDLDLVVNATPWQYHTPIAVAAMRAGKDTATEVPAALTVEECWELVETSEKTGKVCS
                     LLENDCYYRETLLILTMIRAGVLGEPLYAEAGYLHDIRAVKFNTVPNGEPWRLDHTIR
                     RNGNLYPTHPMGPMAWWLDINRGDRLTSLVSMSSRPGAMKEFAIKNFGPDDYRSKTDY
                     KLGDTSVTLLRTEMGRAVTLWHNTNTPRVKEHLLRIQGTKGVFNSLMDKAFVEGRSNR
                     ANDENWRGRHEWEETAEYRKQYDSKLWRTREEEAKGSGHGGIDFFVMSRLIQNLRAGR
                     PVDIDVYDAAAWSVITPLTETSVANRSKPMEIPDFTRGKWKLRAPIDPDLIV"
     gene            complement(26984..28183)
                     /locus_tag="VLE22_27985"
     CDS             complement(26984..28183)
                     /locus_tag="VLE22_27985"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSB18324.1"
                     /translation="MWSSRRTFLAGGLGATLTSCSKPPAPTAEKSASTKLVLEEFQPK
                     SMLVVPEHPIVKAKYPVIDVHTHLASVFGRRRAPESGGPSPEAVKQLDQIMQWMDEIN
                     LQTLINVSGGYGDDLKRNVEVLGKYKGHYANCITPAWDKVQEPDYPKWQADELKRGKD
                     AGAIGLKIAKTLGLYLRERGKEGPLVKVDDPRFDPMWEAAGELKLPVVIHTGDPDAFF
                     TPIDRFNERWEELANHPDWSFYGKDFPPKAELLAARNRVIERHPKTQFIGLHVANHPE
                     NLDEVSTWLKKFPNLHVEIGARIGELGRQPRRSRKFFGEFPDRIMFGTDATPNGKNVP
                     QQDLRPPMFQAYFRFLETEDEYFDYAPSEVPPQGRWRIYGIGLPDAILKKVYHNNAAK
                     LFGMKTI"
     gene            28372..28977
                     /locus_tag="VLE22_27990"
     CDS             28372..28977
                     /locus_tag="VLE22_27990"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSB18325.1"
                     /translation="METTQPPRGRTVPAVNPPLRGASGTAGEPPRGAVPALSEAEKKN
                     ARRRVVWTAVIGFLSASFLMFLRFFFPRTLFEPSTLFRIGFPSDFGLGVDERFKQSHR
                     IWVVRQTDKLFVIYAKCTHLGCTPNWIETENKFKCPCHGSGYDVEGKNFEGPAPRPMD
                     RAHVEVDPEGQIVVDTSRLYQCPPGAVCQFDTDPNSYLIVS"
     gene            29043..29921
                     /locus_tag="VLE22_27995"
     CDS             29043..29921
                     /locus_tag="VLE22_27995"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HSB18326.1"
                     /translation="MEHRPEESRGVTELAEKARKEIAGLVEDLKQPQKTQLWKSVFRV
                     KHERSDTRNRSLGVLSNVFLHLHPARINRDAVRYSYTWGMGGISFYLFLVLTFTGVML
                     MFYYHPTKVQAFRDVLYLEHDVPFGKLLRNMHRWAAHLMIITVWLHMFRVVLTGSYKK
                     PREFNWCVGVILLVLTLLLSFTGYLLPDDQLGFWAVTVGTNMARATPLLGSEGPFGPE
                     LGMTPFNDVRFALLGGSIVDANALLRAYIWHCLAIPIIAAVFMGVHFWRVRKDGGISG
                     PAPVMLESEMKVEHSR"
     gene            30005..30940
                     /locus_tag="VLE22_28000"
     CDS             30005..30940
                     /locus_tag="VLE22_28000"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18327.1"
                     /translation="MDWAQLWEIVSLPDNVPIVALAILVPFYAWYGLRQARATDRLTV
                     ALEADPQLAKTHHRKVHPYKPGWAREVQVWPYLLRIEFLAAVIVTLVLMIWSITLNAP
                     LEEPANPNHTMNPAKAPWYFVGLQEMLVYFDPWFAGVVLPTLIIIGLMVFPYIDTNPL
                     GIGYYTIRQRKVALAGFGVGFVIWLLLIFIGTFIRGPGWMWFWPGQTWDHTRVIYEVN
                     RNLPDLFGISNPVVGAILGILVIGLFYAGSGWGIHKLVTRSTLDRKIYERTSLLQYLT
                     FQVFAITILVGLPAKMLLRLLFRIKYVLVTPWFNV"
     gene            30979..34182
                     /locus_tag="VLE22_28005"
     CDS             30979..34182
                     /locus_tag="VLE22_28005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18328.1"
                     /translation="MPDEINREVRSTDAEPDPVVDSSLSGPLLIAAVVLFLTMVWALY
                     DELFSQRPWKHYQAQFVRLYTAHLQKVGPRAAAAEKEARSSPEFQKIEQLLKKAEDSA
                     APKVKEIDREVDGIRRQLAAIKSPFQDVRARIAALTYELDHAAGKAARDSIRREVDEI
                     RKRQMSVDLPADGAGAAVSAQNLSFVDLEKRFNELRTREAQLISERATVTNQARELRR
                     TRNEYLSEHMVGLGPQQIEGLLRKMHGFRIEIKQIHIEEAGLVDRCESCHLGISEPLP
                     LTAADMGGNRVFVSHPNRTLLTLHDPIRFGCTPCHNGNGLATASVEGAHGNYKHWLWP
                     LFPKENSEAGCLQCHFTDRVLDQAPVLTRGRDLFELKGCVGCHRHEEFDRESDALTSI
                     RSNIQNLEVQQKESRLEADREVQRGDTAESNDQAQQHYAKAERLRVTSSNMDARIKEL
                     DLQAKSVMQDLKKVAPNLKDVRLKLHKNWIPVWLKNPHAFRPGTKMPRFRLVDQEVRA
                     LSAFIWQSGWQGPAPAPQPKGDAAHGKELFETRGCMACHSMGEGNNRAGGEFAANLSR
                     LGEKANYDYIVRWVHNPRDRTRPYCPYEKRDLGPEDYARHGLPFSFGLDRSTCPNDGH
                     ELQVQNMTVMPSLRLTTEEARDIATYLTSLKHDDAAYPSDVAFMDDRRLAEQGRQLTS
                     RYGCGSCHEIAGLEDAPRIGTELTKEGSKPLEQLDFALLEQKAKAEGWYNHKGFFERK
                     IKDPAFFDTGREKALEDRLRMPNFELSPEDSRALITFLLGSLDSPFRSEFRSIPQQFR
                     YIPTDQQKDLQEGWWLVKKYNCMGCHTVQAGQHSVLSALPRYQDPDWKEQLPPSLVQE
                     GARVNPEWLARFLSNPAMTEKDTSRNGVRTYLKARMPTFSFSPNEIRILVRFFEAAAG
                     QSSPYIPSKLEPLTEPERQMARALFSSPGAPCLRCHLVGEPRHDRSATAPNFLVARER
                     LKPAWTARWMLDPQMISPGTAMPSGLFRREDDRWVSAGPTPGIFSGYRGDHVDLLVRY
                     MFQLTPQEQSALIQRLPATARTDRPPPDETATVAHSR"
     gene            34252..35106
                     /locus_tag="VLE22_28010"
     CDS             34252..35106
                     /locus_tag="VLE22_28010"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSB18329.1"
                     /translation="MIALTAVAALALMPGCGGKKETTPAAGEQPAAGGEVWRPTGDEG
                     TVTGKVSFKGQPPKFPAISMDADAVCAAKHSGPVHPEAVIANPNGTLKNVFVHVKSGL
                     EGKTFAVPTEPVVLDQVGCLYNPHVLGIQARQDLKVVTSDNTTHNIHPLPKINREWNI
                     SQPPHADPIIRSFSRPEISVPVKCNQHPWMRAYIHVVSDPFFAVSDDQGSFEIKGLPP
                     GNYEIEALHEHYGAQTQKVTVAPKGSASVEFSYNATQAYRAGSLRMMPALVIPCCGGG
                     GTERGASH"
     gene            35090..35995
                     /gene="cyoE"
                     /locus_tag="VLE22_28015"
     CDS             35090..35995
                     /gene="cyoE"
                     /locus_tag="VLE22_28015"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HSB18330.1"
                     /translation="MARATSAELAGRALPLEGRKLLAYLELTKPRVTVMIFLVTIAAF
                     RLGSPAQTDGWRLLCTVAGVGLLAGGIFSLNQYIERDIDGVMRRTGMRPLPRGELKPR
                     EALRFGLALTILGVAMLALLVNALSAVLAVCTLVGYLFVYTPLKTRTPYCTLAGAFPG
                     AMPPLLGWAAARGEMGVEAWALFAVLFFWQFPHFHAIARLYREDYERAGVRMLPVIEA
                     SGNRTGRQILLTALLLVPASLAPALLGLTGSVYLGGAVLLSLWFLYHGVQSFRVASPR
                     QAQRLLGASIVYLPALFLLMVLDAR"
     gene            36014..36808
                     /gene="coxB"
                     /locus_tag="VLE22_28020"
     CDS             36014..36808
                     /gene="coxB"
                     /locus_tag="VLE22_28020"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HSB18331.1"
                     /translation="MAMVLALFFWLLAVVTAGIFIGRVWWLPELVSEHGAAVDRQLAL
                     TLLIAGIVFFAAQVGLGWFIWRYRARGTGRASYWHESPKLELTWTLITAVVFIGLAIQ
                     GNRVWAKYFLDDAPPDALTIEVTAQQFAWNIRYPGPDGKFGRTDPKLMNDALANYIGL
                     DPNDAAGRDDILTQNLMAVPVGRPVRVIVRSKDVTHSFFVPQLRVKQDAVPGMAIGIH
                     FTATKVGEYEIACAELCGMQHYKMRGRLLVMDSANFEKWLKQRAGA"
     gene            36821..38584
                     /locus_tag="VLE22_28025"
     CDS             36821..38584
                     /locus_tag="VLE22_28025"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HSB18332.1"
                     /translation="MADHEHRHEAPKGFVRKYIFSTDHKVIGIQYLMLALFSVWVGMI
                     LSVFMRLRLAWPEEKWPLLQRVFQEGFAGGPMTPEFYLSMLTMHGTIMVFFVLTTAPL
                     SGFGNYFLPIQIGARDMAFPVLNMLSFWVTFVALVVMMLALFATGGAPISGWTAYAPL
                     SALGEVAGPGLGLGQTLWVISIAIFCGASLLTALNFLTTTLNLRARGMSLMRMPLTVW
                     AWFTTAILALLAFPVLLSAGILLLMDRLGGTSFFIPGGLVVSDQMIAHQGGSPLLWQH
                     LFWFFGHPEVYIAILPGMGVTSHILSTFSRKPVFAYKAMVAAIFAIGFISYLVWGHHM
                     FMSGMSPYSSMVFSITTLAVGVPSAIKTFNWLATLWGGRIRFTTPMLFALGFVSLFVS
                     GGVTGMLLAQPAVDTFLHDTYFVVAHFHIIMGVAAIFGIFAGMYYWFPKMFGRMMSEG
                     MGKVHFWLTIVGVYAIFMPMHFLGVEGAPRRYAQLTEFQYLRHLQPLHLFITVAAFAT
                     VAAQLIFLFNFFWSMKKGRTASENPWESTSLEWTVPSPPPHDNFGDVEPVVHHGPYEY
                     SVPGAAKDYVLQTAPAEEVTR"
     gene            38595..39275
                     /locus_tag="VLE22_28030"
     CDS             38595..39275
                     /locus_tag="VLE22_28030"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HSB18333.1"
                     /translation="MATASKATKRAGGWSGLGEGFGWNGDGSGRERPMGTPPPDVHRL
                     GMWFALCAITMMFIGLTSAYVVRQGIDPEWRPIHMPALLWLNTAVLAISSFTIELARA
                     AQGVGVDGRRVWLRTIPVGGFTSWVAATLVLGLAFLGGQAVAWRQVAGQGIYLATYPH
                     SSFFYLLTGLHGLHVAGGVAALAWLFARGANFSPLRRRRWTEATALYWHFVAGLWIYL
                     LALLFVRR"
     gene            39306..39938
                     /locus_tag="VLE22_28035"
     CDS             39306..39938
                     /locus_tag="VLE22_28035"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HSB18334.1"
                     /translation="MTERAVRAQSATIWDGGVHPFAVSHKKLGMWLFIASDSLTFSAL
                     LVAYSYVRLATEHWPTPFHLWPSISMATIMTFCLLSSSVTMVMGVAASKRGEKARAIR
                     YLLLTMLGGTAFVVLHLNEWLGLIREGVRLWSNPWNVPLFGATFFTLTGLHMVHVATG
                     LIYLGVIAAGVSKGRFGHEEVEVSGLYWHFVDLVWMFIFPLVYLLSVSAA"
     gene            39961..40278
                     /locus_tag="VLE22_28040"
     CDS             39961..40278
                     /locus_tag="VLE22_28040"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HSB18335.1"
                     /translation="MSIGIEETHAPHGASLYYWVWIYLLAITALEVFLAYEHLFSTQV
                     MLAVLMLLSVVKAGLIMAYFMHLRFEKAVFVLCLVPAVVVVISLLAAFFPDSYRLLEL
                     RVR"
     gene            40275..40559
                     /locus_tag="VLE22_28045"
     CDS             40275..40559
                     /locus_tag="VLE22_28045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18336.1"
                     /translation="MMTRRLWQSIVQTLVRVIVLVVAQSSLFGQCSMCRAAAAAQGPE
                     AAKALNEAILILLLPAVALFCGAFILAMRGNRSSDPDESRREGAPGSWPQ"
     gene            40547..40867
                     /locus_tag="VLE22_28050"
     CDS             40547..40867
                     /locus_tag="VLE22_28050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18337.1"
                     /translation="MASMTLTMWQQAIIYLLCLIVAVGSIGTALWALLTGQVGTEGMD
                     GLFLIAVSLLFAFTFSLTPIQALRRGFLRDLLASRKAAAKPREERDAGALPADEAHER
                     TPSP"
     gene            40864..41694
                     /locus_tag="VLE22_28055"
     CDS             40864..41694
                     /locus_tag="VLE22_28055"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSB18338.1"
                     /translation="MTSERRRWLVLAALAVLAVIVPFTVWRETWFGRPLSDDEIGRYL
                     NSTDRARKVQHALSQVSERIVRGDRGAERWYPQVVSLAGHPLAPVRATAAWVMGQDNR
                     SDAFHRALLERLRDSDLMVRRNAALSLVRFGDGSGKAEILAMLHPFTVRAPKAGKVSV
                     GLESGREISQGALLARIGKESASPVEVRAPFSGRLGVVLIPDGTPVAEGEPLMIINPA
                     SEYVWEALRALYLVGQPEDLPDVERCTRVIAEMPESVRRQAEYTARAIRTRSERSSIR
                     "
     gene            complement(41657..42067)
                     /locus_tag="VLE22_28060"
     CDS             complement(41657..42067)
                     /locus_tag="VLE22_28060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18339.1"
                     /translation="MGPELAIVIGKLEPMSHGRTDLRKKKPKQLAVINDNCTGCAGAP
                     VCVAYCPVEACMFWVPDPDHEPFGRIEVDKATCIGCAKCTSKGPDGTFLDGCPWDAID
                     MVAVEEWEGGHGIELPSVPDRPVSEWNSVPSAYV"
     gene            42229..45465
                     /locus_tag="VLE22_28065"
     CDS             42229..45465
                     /locus_tag="VLE22_28065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSB18340.1"
                     /translation="MYKGIAHVLLGMAIVLSFATLVIGQETTGAILGVVRDNTDAVVP
                     AAKVTAISEDTGAQFATETDATGSYQFPLLRAGRYRISVEHPGFQRMQRDGVIVNTTE
                     RVRVDITLKVGVVTETVTVTGEVPLLQSAQVTMGHVVEQRTITSIPLATRNFTQILGT
                     SAGVIGAIYNADQPGTGSDSVSVNGMRRGSNNLLVDGAQTTNPLNMAPDGDGTPSIEF
                     LSEFKVLTSLYSAEYGRNAGSVINVTTRSGTNEFHGSVYEFFRNTKLNARPFFNPTRG
                     QNNQNQFGGHAGGKIIRDRTFFFAGWESSRQVNANSGSATLRTIVPTAAQRAGNFGSK
                     TITDPTTGIPFQNNTIPADRLNPISLKLQEKYIPTPNFSSGATNFFAARGLPTDLNQY
                     TVRIDHRFSEKDTIFGRWFDSRQKDFSPFTWGFPGFGNYANRKKYSLNVSETHLFSPT
                     LIMETRFAYDQTDQYTVPENLDSFDSVGLKPLSVTRTDYGLPQFNINDYQTFGNYQNW
                     SDYIKRFTGSASFTWLRSSHSVKFGYESVNALYNPKNTLDSRGRFFYTGEATGDSYAD
                     FLLSITRSKSFGAGAGELKMRDSVSSVYISDEWKVNQDLTLTLGLRHEAHFHPAAYNL
                     GMTNWYPDRYKGVGSLEASGVVQGGVGGIPQSTINGDWNNFMPRVGAAWRLTDSWVIR
                     AGAGLYFDQRSGQIAQQAFSNPPTFKSIAPDCSVAGSGCTLKEPDNFTFLDPQYDPQF
                     IPFPTKVTDSLAWRGIERDTRTDNAWQWNFNIQRQLPKGFLVEGAYVGTKGTHLMARR
                     NVNPLVPAGFDPNNPKTGTLTRRYPGFADILITAQNGDSIYHSAQFTVKRRIATGTMQ
                     IAYTIAKTLSNGAEGNRYFTSIYDTPWWDFSRARGPANYDRPQRFTAVFTQDLPKVGQ
                     SAVARHVLHNWSFNGFLVVQSGTPLTVTNRDSGRNLGGSAQETARALFSNVAADQPLV
                     NSGSTKDNLKNYINKAAWSKAAVGTVGNSGRGMFRGPGQWNLDFSMFKDIPLTERFKL
                     HFRSEFFNLLNHANFGDPTTNLDSSSFGQISSTAVNARLVQFALRLNF"
     gene            45540..47216
                     /locus_tag="VLE22_28070"
     CDS             45540..47216
                     /locus_tag="VLE22_28070"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HSB18341.1"
                     /translation="MKSQARPSPKANVNVPTPRLEFRDITSSTGITALNVYGGDKTKR
                     YILEMNGNGAAIFDYDNDGWRDVFLPSGTRLDPIEGAEKPTNRLYRNKGGGAFEDVTE
                     AAGLIRTGWGQGACAGDFDNDGHTDLHVTYYGQNLLYRNRGDGRFDDVTEKAGLAAKW
                     ARWSTNCSFFDYDRDGLLDLFVTSYLAVDLKEIQPPGSTPYCAWRGLAVFCGPRGFPT
                     GENLLFHNEGGGRFRDVTRRAGIALPDLHYNLGAVTADFNNDGWPDIHVASDSSPGIL
                     YRNNKDGTFTDVAVLAGVAYGDAGQEMGSMGTSAGDYDNDGLIDIVKTNFMDETPTLY
                     RNRGNLFFDDATYMAGLGVNTKFVGWGVDFLDVDQDGWKDIFMSHGHIYPELLASDVS
                     EPYRQRKALFWNVRNGAFRDATEKAGDALTTPNVSRGFAAGDLDGDGTPEIVVVNMNE
                     RPAVLKNTGERGNAILVELIGTKSNRSAIGARIKVTAGGLQQIDEVRSGGAYASQRDF
                     RLHFGLAAADKADVRVQWPSGAVEEIKGATANQWITIREGEGVIKRTPFR"
     gene            complement(47229..48668)
                     /locus_tag="VLE22_28075"
     CDS             complement(47229..48668)
                     /locus_tag="VLE22_28075"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSB18342.1"
                     /translation="MVRITGQFVFASGVAFSTRPRIAGKIDRVNVRSALFLLALPLCA
                     QLPDAARARLAEAANLAAGGQSPKAEEILTTLSRSYPRNAEIRHTLGLVLLRAKKHVA
                     ARRELEAAATLDAKSPLIRLALAQARLETGSKAEAMRAAAEALRLAPSEPVVAHALAM
                     FYSQAGEFSKAAEQETRWARANPTDRLALLRTAEYHLRANEAEAAIQLAGEVAAAGPS
                     APAQSILGKAYRLKRDPPKAVAALQEAIRLEPAQPAYVADLAQLFLDHNTPEPARMLL
                     DQAMTRFPGDVELRRMLGLACYAIGNTERALDEFLRVTEMEPDSALSYAALESLLPDA
                     GNRLDLVVGKFRTFAERHPKNPVAHYLLAMGVATRDGATAETEALLRRAIATDSGFWP
                     AWFELHTVLKAQDRFDEAAKALEQTLKLNPDHAAAHYSLAQIYSRLEDTARAAEHRQK
                     HHQLVLRDREETDRKRREMPRLAYEVRKG"
     gene            complement(48775..49980)
                     /locus_tag="VLE22_28080"
     CDS             complement(48775..49980)
                     /locus_tag="VLE22_28080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSB18343.1"
                     /translation="MIRSAVRVAGGVLAGLVVIFIASLAVSPYFRRWALLNLPDVYDY
                     SKLPSRTVPRSVQPFRFPDCTAGDWISPLKLAYSHQSIADEAALGRFLAEHGTTAFIA
                     VKDGCLLDERYFNGFRRDSLFKSFSVTKSVVSALIGAAIADGWIGGIDDPVTKYIPEM
                     QDPRFTRVTLRHLLDNTAGINYERGNMPWTSQPRMYYTTDVRGYVRGVGIDVEPGVRF
                     STEDMSPIILGWALERALRRHPAAPTLSGYLAERIWQPIGAEYDAMWIVDREHGGLEK
                     GESGLTARAIDLAKFAALYLQQGRWNSRQVIPAAWVSESTSVNSDPPGPNVWSSGFFK
                     HQWWGRKVPAPAPSDFYANGHFGQRLYVSPRNQLILVRMGDSNRGIDWADFLAVVADA
                     FVLGPKTGE"
     gene            50037..50723
                     /locus_tag="VLE22_28085"
     CDS             50037..50723
                     /locus_tag="VLE22_28085"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSB18344.1"
                     /translation="MSRSKRSEGSRSAAEAQIPYHKGNLRHKLLREAARMIAKSGVEA
                     VSMRKLSDRLGISRTAAYHYFDNKGELLAAVGQHGFTRLADRVQAAGKPELPPLDNLR
                     QALLAYVRFATEETEFFRLMFANVLQRPVQIGATAELSAFRFSSPEAFAVFDALLSAV
                     KRCQADRCIRGGDPLVVANTLHAFIHGVARLAIDDHLKIQCSMEEFLDQGLDALFSGL
                     GGPAQARLPQ"
     gene            complement(50769..51581)
                     /locus_tag="VLE22_28090"
     CDS             complement(50769..51581)
                     /locus_tag="VLE22_28090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSB18345.1"
                     /translation="MNNVRSFAENHPIGFVVSATIAWFVLVLVFTGIATSALAEPYGG
                     AATATVGRLAVTVCVLLLVWRLGWLKASGVGRPGSRQVWLIALAGLIYFACASLYSFY
                     GKVAVDISNFVRLPAARTTVVVQFTVGLSEEILFRGFVLYSLVRVWGNTKQGMILSVV
                     LASVLFAVLHITQVFTHGVSLPSALLLTLETWIISIWWGALVLLDRSIWPAVMLHFVV
                     SAVVGVQSLTVAMVEPDVLAYTRILWFSIPLGALGVALLARGATHPIVPEAP"
     gene            51843..52808
                     /locus_tag="VLE22_28095"
     CDS             51843..52808
                     /locus_tag="VLE22_28095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18346.1"
                     /translation="MEADSEILEIDRLEDEIAPVTSDVSRVRDLISGLELCHHKAERW
                     VHNIIEAISLGETGKGLGTRPAVQLHPAEQVWQSACDSLSGWCGGYPAASNDLCVGAV
                     PAIRLLAGLGDRSALKEWQVQRVIEKVRSLIHWPLSSDDPAARYVYILLGGGEYEALY
                     RTQCPEHYRKHEDFWLATVRTMIHDTENGRAAALSLGLAIDMLWPCHWRFVENLQIVL
                     DAIGGRLKPDKPFAACGRNISLLPNRNRMLTVSRSLRAFCGHSETGGEVDRGILSLLG
                     QRTAAKSWLAASLDKTIRLQLDPPAGVRAISALTGPDWITRSVGA"
     gene            complement(52849..53337)
                     /locus_tag="VLE22_28100"
     CDS             complement(52849..53337)
                     /locus_tag="VLE22_28100"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSB18347.1"
                     /translation="MAIRRNMLEKTIGNALSGKGSHVATKTLCAGLGWKAAGARPEGV
                     PHSVFQLLNHMSYWQEWVAKWLDGGNPPVPKHAPGGWPGSVGPANAAEWQRAVRGFRN
                     ALAKLDRQSREADLLTTRGKHSRLGMLQAIASHNSYHAGQIVVLRQILGAWPPPSGGI
                     TW"
     gene            complement(53714..54364)
                     /locus_tag="VLE22_28105"
     CDS             complement(53714..54364)
                     /locus_tag="VLE22_28105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18348.1"
                     /translation="MCRNWTQYVCSNAGRVPAALILVAGCWSVTQPLHGQSATLTVGV
                     YDYAGVPHEVLSRAEEDAARILSRAGVKVKWMACPRPHEDAARGFTCTAIREQGGLFI
                     NVLRNAQAKQLGRPHSIYGETVHPSVAYVFAEKARTTAVGRGVPEHTVLGIVLVHEVG
                     HLLLGPGSHSKDAGIMSDGFCLPVFQRAAQGQFPLFTPHQARLIQTRLSERASAVK"
     gene            complement(54474..55043)
                     /locus_tag="VLE22_28110"
     CDS             complement(54474..55043)
                     /locus_tag="VLE22_28110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18349.1"
                     /translation="MKRTRILQLFTLPVAVALLLGLATPPKLDAEFECKQELLTGAWS
                     YIYPQENLVPNGMPQTGSGIVIFDQEGKATYFGHIEVSKDGAAPLMDVTQFLDVQCTV
                     TSDCRGNCVFRIPSTGEVVQETGYVFADGQRLAWGLTTVPPNIGGIVTMKKLDPADAE
                     LEAKVDALGTKIDLIMRWLGLVVPSGPKK"
     gene            55229..57079
                     /locus_tag="VLE22_28115"
     CDS             55229..57079
                     /locus_tag="VLE22_28115"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB18350.1"
                     /translation="MKEPVPKSARHFYSFGAFRLYATERELFRGSKPVTLPPKTCDTL
                     LLLLRRHGHVVDKDELIRTLWPDTFVEEDNLVQQVSHLRKALGERPDGGPYVETVARR
                     GYRFAALVEESWEEPAIAGRRRVPLLFWTIAVPILTAIAAAILLLLNVAGLRDRLLPK
                     PTNPRITSIAVLPLENLSHDPEQEYFADGLTEELITTLGKISALRVISRTSVMRYKRT
                     KKPLPEIARELNVDAVIEGTLLQSGGRVRITANLLHAPTDRHLWAETYERDLGDILTL
                     QRELARTVAKEIRATLSPEEQVHLSRSRRLNPEAHELYLKGQYHYYKWRSAEFAKAVT
                     YFEKAAEADPNFAQAYLGLAKTYGWQWMQGSLPPKEAYPKFSAALKRAMEIDDTLPEA
                     HYVLAVSAWYFYWNWTQAEAEFKRALALNPNLEEARFEYGWFLTTLGRFTEGIAEARR
                     AVEGDPLSFSANLALGSVYVFARQYDRALAQHLKTIELDPNDPRGYEFLAGAYAALGL
                     YDESLKAREKQMTLAGAKPEDVAAMGESYRSSGFAGYLRWRLERVKHPYERAMIQAQL
                     GLKDEAFANLEKCYREHWWAMVQLKSLPSWDPLRSDPRFQDLLRRMNFPP"
     gene            complement(57288..58247)
                     /locus_tag="VLE22_28120"
     CDS             complement(57288..58247)
                     /locus_tag="VLE22_28120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18351.1"
                     /translation="MKKRTNAWPSVSVTAMVAFFLMFGVTPATPADSWTFVSIDVPGA
                     VWTLARHSTPSGAVVGTYKWGPAGWQTRGYLLTPDGFRDISHTAFGRRVDVRGTNSRG
                     DVVGQTSDPAKFLPVYSHGFLLRKGKVIWIDPPPGQCPATTCETIPMDINENGDIVGF
                     YAAYDLPDYENYSEHGFLWSHGVFTTVDATPLGATLTNARGINPDGDIVGLYSEFGYN
                     GPFHGFLRRYDGTFTAIDVPGAIGTSANAINPGGQIVGDYTTPDWIVHGFLLSEGNYT
                     LFDYPDAVETYPFGISANGWIVGFYFESSGALHGFLVSREGLE"
     gene            58757..59089
                     /locus_tag="VLE22_28125"
     CDS             58757..59089
                     /locus_tag="VLE22_28125"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSB18352.1"
                     /translation="MLRSGEQRLDEIPSQGVPLGLFAGEGLIPYETREVHLGKRDLLM
                     VASDGLGDIRNRSGEFFQDSQLRPTPTELSGRDVDSFLEHLVRSATTFSGGQPLLDDI
                     TVVAVTRT"
     gene            59143..59700
                     /locus_tag="VLE22_28130"
     CDS             59143..59700
                     /locus_tag="VLE22_28130"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSB18353.1"
                     /translation="MAVHVCPWWIGYLLACPLRRYSQDPEKILGPYVREGMTVLEPGP
                     GMGFFTLEAARRVGGSGRVIAVEIQPKMLHRLKRRAEKAGLRERLDARLAMPESMNLT
                     GLDGQVDFAFAFAVVHEMPGTDSFFLQTARCLKPDGSLLLAEPRGHVKAEGFEKELAA
                     AARAGFSLVERPVIPRSHAALLKKG"
     gene            complement(59731..60459)
                     /locus_tag="VLE22_28135"
     CDS             complement(59731..60459)
                     /locus_tag="VLE22_28135"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HSB18354.1"
                     /translation="MNSARGAADTMSPTGRPPSLRSSLIRYCVMGLVLASVLRLVSGR
                     ADWLRAWFYVALTLGIQVVVGVVLHRVSPDLLAERSRIRKGTKPWDKVLVPVVAIVGP
                     LAIWCVAAWDVRVHWPPAVSIGWSAAAFAVCTLGSLVTFWAMVTNPFFSATVRIQEER
                     GHRVIDGGPYRYVRHPGYCGALAFTIASPVALGSWLAVIPAAFTAAVLVIRTSLEDRT
                     LRAELEGYREYAQRVPSRLVPVLW"
     gene            complement(60538..61215)
                     /locus_tag="VLE22_28140"
     CDS             complement(60538..61215)
                     /locus_tag="VLE22_28140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18355.1"
                     /translation="MLCNSALAALLGGASHRTLFAGGTRAEPAPEIIIRIYNYADAPL
                     EVVMTAQREAARILGRAGIASRWQDCSSDQHPECQEAPGPVRLNIRILPVARIKLAGL
                     PPGACAYSLVPARGFGVVVSIFWRRVEELAKGLQVFQGLYLGHLFAHEIGHLLLADGG
                     HSSAGIMSREWDPHDLSYAAQHKLLFNQRQADQMGARLAERIHAEQAAIEPKASNFCG
                     SGLPITH"
     gene            61580..>63545
                     /locus_tag="VLE22_28145"
     CDS             61580..>63545
                     /locus_tag="VLE22_28145"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSB18356.1"
                     /translation="MRQSSQSHSACRFGAFEVDLRARELRKGGLKIKLQEQPFQVLAL
                     LLERAGQVVTREELRQALWPSDTFVDFDQSLNTAVRKLREVLGDTASTPRFIETLPRN
                     GYRFVAPLEAIGGKDPAREPKRKKQRRLVWVASFAVAVALGAIAAWYFWPKAPVPERV
                     LSAIPLTSYPGEEKEPSFSPDGGQVAFAWNGDKEDNQDIYVKLIGGGPPLRLTTDPAE
                     DYGPAWSPDGRHIAFVRRRRDSGDLFLIPANGGPERRLAGIQWYGLRIKLRDRQFPLV
                     AWSPDSKWVAFANRDSPEQPYSLFLLSPETGEKRQLTSPPKKSVGDGGVAFSPDGRQL
                     AFVRLMSSGYGHLYVLPLSEEFRPTGEPRRLPFNQPVNMTPAWTPDGREIVFAAGPAG
                     SRLWRVKVSGSQGPQPLGLPGESASGPAVSRQGGRLVYVAGTSDLNIWRMELSGRSEP
                     GPPVRLIASTKVDVAPQFSPDGKRIAFESDRTGNGEVWVCDGDGGNQAQMTSFGAHSG
                     SPRWSPDGERIAFDSNKEGRWQIYVVDAAAGVARRLHDSPFDDDSPGWSHDGKWIYFA
                     SNRTGELQVWKMPAKGGEPVQVTRRGGFLAYESPDGKYLYYMKRFEPISEVWKVPVDC
                     GEEIKVLDAVWRRGFAVTNQGIYFMTVPEAD"
BASE COUNT        12284 a        19731 c        19429 g        12101 t
ORIGIN      
        1 tgccgaaggt agcgctttcc ctgccggtct tcattagcgc tctcggcgga aaaattggaa
       61 cggcgctagc ccgccgtcag gctcccttcg gcaggcggaa cggccggcgg gactgctcga
      121 cttcattgcc gcgcgtccag atcagatcga tctcagcgac gccgtatccc ttgcggtttg
      181 ctagttcgag gtgtttcatc cgagccggat cgatgccaat caccgaggcc cccaccgtgt
      241 ccaccgccag cgcgctggca cccgcgatga cgacgttatg atgcactccg cgcgcatccg
      301 gtccgtaggg accgtcgccc tccaggcccc agccgccgcc cagaatcgag tagtcagacg
      361 cgtggtatcc gtagagatcc accaggatct cgtgcggttc gccgagttgg aacacctgct
      421 cttttgtccg cccgtacttt gtctccggca gaatgccaaa gtagctcccc agggacagcg
      481 cagccagcaa cctggcgtgc gtcttcaacg gcgcaatagt gatgagcttg tcgcactgct
      541 gaattgtcga ggggatgaag aacacgccct ccttgttccg gctcgaggaa ggccggcccg
      601 gaatcggcag ttcgagcgct ttgtcggcgt tcagatcgac gacgtcgaac ttcactccgg
      661 cgtgcttctt tgagaactcc tccacgacct tgcgataggt gaagcccccg aacgcgccgt
      721 tccaatccga cgtccaaact tcgaccggtg tctccggcct ccacgacggt ccttccgcga
      781 tcgtgatgcg ctcgcccagg cgtttctcga ccagccacgc gatcaggctg gacacaagac
      841 gcgggtcggt gatcgaaccc ggcacaatgc gcccctcacg gtcgtggcag acggcgatgt
      901 ccggcttgat caccacccag tcggagggct cgacgatcga ttcgatgtcc tggccgcgaa
      961 cgccgctcag ctccacggct ttgcggaaca tagcatccag ttcggacgcg tttaaggtgg
     1021 cgtccacggg cttcggccgg gcaagtccgg tgagcttggt cccgtcgtgg tccgagcctc
     1081 cgtcgaagct ggacagaacg acgccgacgc gaggtgtctg gtcggcggtc gcgctggcca
     1141 ccacctcatc atcttttgtc tccagctgct gctggcgggc agccgcgcgg gccagtccca
     1201 caccggccaa ctgcacgaga atctctctac gcgactgcag catgtggtta cccctgcccg
     1261 gcaggactgc cggaatgagg cattatacgt taaccaagcg gcgaaagcct cggtcgaagc
     1321 gcgcgaagcg gccgacggag gaaatgcgcg gggcatcgtt ccagactgcc ccgcgccacg
     1381 agcctctttt gctgtagggt tcggctatct gcggccgcga cgctgacccg atcccgttcc
     1441 gccgcctgtg cccggctggt ggatgggtcc ggacccgtcc cgtggcccca gtttcttgcc
     1501 gttgccttgg ccacggcgtt ggcactggac ctgagtctgg ttcggattct ggttgccgtt
     1561 ttgactgaac gcaggagtat cgaacgcgca gactgaacac gcgctgacca ggaacatcgt
     1621 tgcgactgcc agggaactct tcctcttcat attgatggtc tcctttctga ccttcaccct
     1681 caagaacgca acttgggttc cacggacctg cgcaagtagc agcaatcact taacgacggt
     1741 cgagaggctt cagacatgca aacgccgcgc tgagtcacgc aagccctgcg cggtcacttt
     1801 tgacgctgaa cgtgaacgtc agatccgcct ggctcagtct tttgtctgcg cgatgcagta
     1861 ttccttcaag cggtattgga gcgttcgcag gctgatgccg agttgtctgg ccgttttggt
     1921 tctgttgccg tcgttcgttc ggagtgcatc ctcgattgcc tggcgctcga tgtctttgaa
     1981 aagcaccggc cgcgccggac cggtggtggt gacggggagg tcgtccggtt caatcacgtc
     2041 atcgcacagt atggctacgc gctccatggt gttctcgagt tcgcgaacgt tcccgggcca
     2101 accgtaggcc agcagcacat tctcggcttc cggcgtgagg acgaggtggg gcttaccaag
     2161 gtttcgtgct gctctgccca gaaagaaacg agcgagtttc gggatgtccg aaggacgctc
     2221 gcgcagcggt gggacctcaa tgggaaacgt attcagtcga tagtagaggt cctcccggaa
     2281 cttgccgtct gctacgctct gcctaaggtt cctgttcgta gcggcaatca cgcgcacatc
     2341 gacggcgatc tcccgcgtgc ccccgacgcg ttcgaacgtc ttctcctgca agactcgcag
     2401 cagctttgcc tgcagattcc catcgagttc tccgatttcg tcgagaaaca acgtgccagc
     2461 gtgggccctt tcgaagcgac cgaggtgctg gccggcagcg cccgtgaacg agcctttctc
     2521 gtgtccgaaa agctcgctct cgatcaagga gggtgaaaga gctgcacagt tgacggcaac
     2581 gaagatcttc tcagcccgtg ggctattttt gtgaatgcag cgcgcgatga gctcctttcc
     2641 cgtcccgctc tcaccgctga tcagcactgt ggtatccgtg ggagcgaccc gccgggtcaa
     2701 ttccagaacc cgctccattc gcgggtcgtc cgcgacgaga tttccgcacg agaaacggtc
     2761 ggcctcctct tcgcggagta acgcatagtt tccggccaat tggcgctggt cgagcgcctt
     2821 gcgaacgagc agccggagtt cgtccggact gctcagcggc ttgcctaggt agtctaccgc
     2881 tcccagtttc atggcctcca cggcggatgc gattgtcccg aatgccgtaa tcatgatcac
     2941 gggggtctcg ggcatgttct ccatccacca tcgcaacacc gccagcccgt ccccgtctgg
     3001 aagcttcatg tccgcgacga cgaggtcgta gttacggcgc cctccggcgc gcacggcctc
     3061 ggccaccgag cccgcatttt cgacaccgta gccttctccg acaaggatcg tctccaacag
     3121 attccggaat cccggatcgt cgtcgataac aagaattcgt tccatacgtt ccctcttagg
     3181 acgcctcgaa ctccttggcc tggagaccgg ggaagtggag gatggcttcc gtaccgtgcg
     3241 ggtggccagg gttcatcgaa agctcgccgc ccagtgattg ggccagcctg cgagtgatcg
     3301 ccagtcccag acccgtcccg aaggccttgg tcgtaaagaa aggctcaaag agcctcgact
     3361 gggcgatttc cgacagaccg ggcccgttat cgatgaccga tagaatcagt ccctgcggcc
     3421 tgtggctgcg gatctccaac ctgacctcgc cgccggctac gtcgccaatc gcgtccgccg
     3481 cgttgcggac aaggttcagc aaagcctcct gaagaaggtg cgggtcggtg ttccattcga
     3541 gctttgggtc gtcaggtaca aaccggatat cacgagtccc tatgatttgt cgggcgtgtg
     3601 cccgaagtgg accgagtgtc tcttcccact ttgctatccg aaccgaaggc gcaggaggcc
     3661 tgccgtaaag cagtagatca tggaccaagc cctccagccg ctctgtctca cccagaatcg
     3721 gctccagaag ctgccgaaga ggcgcatccg ctttttcgcc ggcgagttgc gcgaatcctt
     3781 tgatcgttcc tagaggattg cggatctcat gcgccaacac ggcagccatc ttacccatgt
     3841 gagcgagatg ctcgagctcc agttgccgaa ttttgaactg cgccgtccga cgcgccgccc
     3901 agatcccata cgacgacacc atcacaagaa cgatagcgcc cagtgaggca acgataacgt
     3961 tgtggcgtgc gtgggccacc agaaaatcgg cggccctgcc gtcgagatca atgcgtgcga
     4021 tgcgcagacc tgtgtcagag tggaacggca cataggcgcg gtaaaccggc tcctggctgt
     4081 ctccagtgaa cccagctcga aacaattcct gcccattcca gatcggctgc aggcgccgct
     4141 catctcccgc ttttccacgc tcaatcaatt ccagtccgat gaggctgggt tcgtccttgc
     4201 caagctgttc gaaaacgagg gttcccgttt cggaaatcgg aacgttctcc agacgggccg
     4261 ctagcatcgc tacgcgagtc cgaaggtaga ctaccttctg ctcatccagt tcctggaacg
     4321 tgcggacgga actcaacagg agaaccagcg gcaatgctac gatcaccacc ggcaacagca
     4381 ggtgggagag aatctgggtt ctgcgcgaca ctctacccat gctagatgca ccctgagtgg
     4441 tcatcgtgac attcctgcac ggagaatgga cgttcgattg ctcgtgggaa gggcggagat
     4501 cgtatgagaa gtgtccaatt catagtgctg accggccttg tcgtggctgg cgtggcgctc
     4561 gggcagtcat cggagtccca ggcgccgatc gctgacaatc ccaaggttga ggtcaaaggg
     4621 aagatcgaaa agatccggat cgcccgaggc cagggaatgc cgtccctcga cttgaatacg
     4681 ggcgacagga ccgttagcgt catcttggga tcgatgcggt accttgtgga acaggatttc
     4741 aatcccaaag ccggcgagga ggtggtggtc tccggttaca aagtgaacga cggcattgtt
     4801 gcggtcaccg tgactctcgc ggcgactggc aggcttctga ggctgcggga cgacggcggc
     4861 cggccgcttt ggattggagg gcgccgtgga gcgcccatgc acaggcgcgc caaatgagcc
     4921 ggcgatacgc cctcttccgg attccgccgg agctcctcta ggaatcgcgc aagacctgca
     4981 ttctgagcac tgacactgcg cagaagttgc gtgacgctcc gcggccggat ctgctcaggc
     5041 acgatcacgg gaagaccatc caagacctgc gggacgcagt ggcacattgc cgagaagagg
     5101 gtggtagact gggcgatcaa gtttggtcac gcgcagcgcc ctgcattagt tagaatgagg
     5161 gaaatctccc gtctccgaag gcggcactca tgtccggcta ttaccaccag gaagacctgc
     5221 ctcgcttttc cgaaatgggc aagaatgcgc ccgacctctg gaagaaattc gccgagtggt
     5281 acggagccgt cttcgccgag ggtgcgctaa cggcgcgtga gaaatcgctg atcgccctgg
     5341 cggtcgcgca cgctgtacag tgtccgtact gtatcgacgc ctatacccag gactgcctgg
     5401 agaaaggctc gaacgtggaa cagatgaccg aggccgttca tgtcgcctcg gccatccgcg
     5461 gcggcgcttc cctgattcac gggctccaga tgcgaaacgc cgcggagaag gtgagcatgt
     5521 gagcctggtt tcgatcggac atccgctcgc ttcggccggt gagcagcttc gcgtgctcag
     5581 cgagccgcga cgccagctca agccgttcga gcagtcgctc gccgagtctg gattgttgcc
     5641 gcttacggcg acgggcatca ccgtgcttca gatcaacgtc ggtaaactct gcaatcagac
     5701 ctgccggcac tgtcacgtgg acgccggtcc ggaccgccgc gagacgatgg cgcgggagac
     5761 ggcggaacat tgcatccgcg tgctggcgca gacagacatc ccgacgctgg acattaccgg
     5821 cggcgctccc gaactgaatg cgaactttcg ctggattgtg gaacgggcga agacgctggg
     5881 ccgtcacgtg ctcgaccggt gcaacctcac gatcctgacc gtgccgtcag aggcggatct
     5941 ggccggattt cttgcagccc gcgaggtcga gatcatcgcc agcctgccgt actacctgga
     6001 ggacgaaacc gaccggcagc gcggcgaagg ggtcttcgcg aaatcgatcg aagcgctgcg
     6061 cagtttgaac gccctcggtt acgggcagga gggctccggc ttacgcctga acttggtcta
     6121 caatccggtc ggcgcgttcc tgccaccggc tcaggcggct atcgaagcgg acttccggcg
     6181 cgaactcctg cggcgccacg gcatcgtctt caattcgctt tacacgatta ccaacatgcc
     6241 gatcagccgc ttcctcgagt tcctgcttcg cacgggcaac tacgaccgct acatggagaa
     6301 gctcgtcagc gcttacaacc cggcggccgc cgctggcgtc atgtgccgtt ccatgctctc
     6361 ggtcgggtgg gacgggacgc tctacgactg cgatttcaac cagatgcttg agcttcgcgt
     6421 cgatcacggc gcgcccgccc acatccgcga cttcgacccc ggcgcgctcg cccgccgccg
     6481 tatcgtcacc ggccagcact gctacggatg caccgccggc gccggctcca gttgcggcgg
     6541 cgcgacggcg taggacttta aagactgctg aatttctcca cagaagctgt cttcgcctgc
     6601 ccgctctccg caaccacaca accagcccgc ttccccctcc aagggaggat tcggcccgtt
     6661 cgatgattcc caattgttgc aatcgattag gtggtgcaaa ctgtacggga actgggaaaa
     6721 ccctggatct cccacaagcg aggtaaggta tgagcactca gactgtcgag ccgaagccca
     6781 gccccgtcat gggtctggtg aacatcgact cctcgatcga ggatgtcata cggcagcgtc
     6841 tcgcggaaga acgcgcgcgg ctggagcggg aagacgctgc tgaaaagcat aaggttcacc
     6901 acttcaagcg gcccgcggag cgctcgttca ccgcagacca gcgcggccac accacgctgc
     6961 tcttcggcgg cctcacgtgg aaacacgaga agctgatcca tggggcgctt gaaggccttg
     7021 gctacatctc cgaagctatc cccacgccga acgtcagcgc cttccagacc ggcaaggagt
     7081 tcggtaacaa tggccagtgc aacccgacct acttcacggt cggcaacctg gtccagtttc
     7141 ttcagtccct tgaggaacgg gggctcacca aacaggagat tatcgaccgc tacgtgttct
     7201 tcaccgccgg cgcctgtggt ccgtgccgct tcggcatgta cgagtcggag taccggctgg
     7261 ccctccgaaa ctcgggcttc gacggcttcc gcgttcttct gttccagcag tccggcggcc
     7321 tgagccagtc cgatgccgag gccggcctcg agatgaatat cgacttcttc ctcggcatcc
     7381 tcaacgccat gaattgtggc gacgtgttga acgaggtcgc ctatgccatt cgccccttcg
     7441 agacgtcgca aggcgatacc gacgctgtcc tcgaccggat catggactat atgcacgagg
     7501 tgtttcgcaa gaaacagccc tggacggtgg gcgacaactt ggcgaaatac ctgggcggct
     7561 tcaaggactc caccgagtat attgggaagt ttgtcaacca gttgaagggt gacgagtaca
     7621 ccgcgtccct ggaacgctgc cgcgatctgt tcaacgagat cgaggtcgac cggctccgcg
     7681 tcaagccgat cgttaagatt acgggcgagt tctgggcgca gaccaccgag ggcgacggaa
     7741 acttcaatat gttccggttc ctggagcgcg aaggcgcaca ggtggttgtc gagcccatcg
     7801 gcacgtggat catgtacatg atccaccaag tcgttcagaa gcaccgcgat ttgaaagggc
     7861 tgagcgaggg cgccgttctg ccgcccctct ggcggctcga caagcgcgcg aagatcgaat
     7921 ggagcttccg gaacaaggtt gccaagctga gactcgccga ggcgatcttc aagcgcgaat
     7981 accaccgagt cgtcgatgcg cttggcggca tcgcccacca tctcgccgac cagtacgaac
     8041 tccagcgtgt gggccacccc ttctacaact ctcgggccgg cggcggcgaa gggcacctcg
     8101 aggtcgccaa gaacatctac tactcgaaca aagatctctg ccacatggtc ctgtcggtga
     8161 agccgttcgg ctgcatgcct tccacgcagt ccgacggagc ccaggcggcg gtcgtctccc
     8221 agtaccggga catgatcttc ctgccgatcg agacttcggg cgagggtgag atcaacgctc
     8281 acagccgcgt acagatggcc ctcggcgaag ccaagaacaa ggcgaagcgt gaattccagg
     8341 aggtgctcga ccgcaagggc ctgacgctcg aagagtgccg cgcttacgtt gacgcccatc
     8401 ccgagatgaa gcggccgatg taccgcgtac cgcataccaa gggcgtcgtt ggcacggcgg
     8461 cgaactttgt tcatcacgtc gcggagagga tgggccgcgc ctgagctcga accttgaacg
     8521 cgcccattcg cgttcatttg cgcccttgac gtgaaaacta tggacacacg atttctgatc
     8581 ggactcgatg taggctcgac cacggtgaag gcgatcgtca tcgatgcggc caccgacgag
     8641 atcctctggc gcgactatca gcgccacgac accaagcaac ccgagaaagt ccttgaattc
     8701 ctcacgcgct ttgaggcgga aattgacggc ttcgagcaac aggcctcacg gatcttcgtc
     8761 accggttcgg gcggctcggg cgtcgcgaaa cacatcggcg cgaagtttgt tcaggaagtg
     8821 aacgcggtct ctctcgccgt agagaagctc tatccggagt gtggttccgt catcgaactg
     8881 ggcggccagg acgcgaagat catcatcttt aaggaagacc cggagaccgg ccgcaaaaag
     8941 aaactcccgt cgatgaacga caagtgcgcc ggcggtaccg gcgcggtcat cgacaagatc
     9001 aacgccaagc tccgcattcc cgccgacgag ctctgcgaaa tgggctaccg cggcctaaag
     9061 ctccatcccg tggccggcaa gtgcggcgtc ttcgccgaga ccgatatcaa cggcttgcag
     9121 aaggccggcg tgccgcccga cgagctgatg gcatcgctgt ttgaggccat cgtcatgcag
     9181 aacctctccg tgctcacccg cggcaacacg ctccggcccg tcgtgctgct gttgggcgga
     9241 ccgaacacct acatcaaggg aatgagggac tgctggaagc acaacatccc gaagatctgg
     9301 gaggagcgga accatccact gccggaaggc gttcctccgg aggatctcat ccgaacgccc
     9361 gacaacgccc agtacttcgc cgccatcggc gcggtcgagt tcggcaagac cgaggacgaa
     9421 ggcgtcggtc aatatctcgg ctggaacaaa ctcgagtggt acgtcactgt cggccgcgag
     9481 gaggagaagg cgaaacgcgg cggcaagggc ggcctggcaa agagcccgga cgagctcgag
     9541 tcgttccggg ggaagtaccg ccacaagaaa ttcgttccgg tcacgtttca gccggacgag
     9601 gtcgtcgagg gcttcatcgg tattgacggc ggctccacat ccaccaaggc ggtgctgttc
     9661 tcgaaggaca aaccgcgccg cattcttgcc aaggcgtacc agctttccaa gggcaatccc
     9721 atcgaggaca cgatggagat cttcgcccgc ctggaggagc agatcaccgg ccagggagcg
     9781 tcgctcaagg tactcggcgt gggcaccacc ggctacgcca aggacatcct taaggacgtc
     9841 atcggcgcgg acgcagccat tgtcgagacc gtcgcccaca cgcaggccgg tctccatttc
     9901 tatgacgatg tggacgtgat ctgcgatgtc ggcgggcagg acatcaagat catcatcctg
     9961 aagaacggca gggtgaagga cttcaagctg aacacgcagt gttcggccgg caacggctac
    10021 tttctgcaat ccaccgccca gggcttcggc tatccggtgg aacagtttgc cgacgtggcc
    10081 ttcggcgcca ccggcttccc gcaattcggc tacggctgcg cggtcttcat gcagtccgac
    10141 atcgtcgact tccagcgcca gggctggaag cccgaagaga tcatggcggg gctctgtaac
    10201 gtgctgccga agaacatttg gctgtacgtc tcgcagatcc cgaacctctc ggccatcggc
    10261 cgccgcttcc tgcttcaggg cgggacgcag tacaacctcg ccgcggtgaa atcgcaggta
    10321 gacttcatcg aatcgcgctt ccgcggcaag gaagtccccg cggacgtaat cgttcacgag
    10381 cactgtggcg aggcgggcgc aattggagcc gctctcgaag cgggccggct gtgggaccac
    10441 ggccgccaga gcacattcat cggcttgaag gcggtgcagg aaatccgcta ccagaccacg
    10501 cgcagcgagg ccacccgctg ctatttctgc aagaacaaat gcctgcgaac gtttatcgac
    10561 gtccagacaa gcgtgctcaa tgagaacggc tatcgtccgc cgaagaccaa ggtgccgctg
    10621 gcggacggtt cgcagcgtct catcatcgcc acctgcgaga agggcaccgt cgaggacatt
    10681 agcgagatgc gtggcatcaa gggcaatctc gacaagatga agaaggccaa tccgaacttc
    10741 gtcgagatcg ccgcgcgcgg tgtgttccgc gttcccgacg cgcccatcgt cgccgacgct
    10801 ccgccgaagt tccagatcac cgccgcccag aagaagcggg cggagctgat gaagcgccgc
    10861 gcctcgctcc gcatcggcat gccgcgggtg ctgaacatgt actcggtggc gccggtgttc
    10921 accggctact ttgcctcgct gggcgtgaag gcggagaaca tgattttctc cgaatacacc
    10981 agcgagcagc tctacaagga aggcggaaag cgcgggtcca tcgatccctg cttcccgtcg
    11041 aagctcggca tcccgcacgt gcacaatctg ctctacacgc agcacgcgaa gaagccgctc
    11101 gacctcatct ttttcccgat gatcgactgc ctgccgagcg atcttgcctg cacgcaagcc
    11161 aatcgctcct gcccgaccgt ggccgccacg ccggaggctg tcaaggctgc gttcacgaaa
    11221 gaaggcgatc tgttcgccga gaagggtgtc cggtacctcg ataccttcgt caatctatca
    11281 aagcccgaca tcttcgagcg gcagatgtac gagcagttcc aggacatcct cgggctgtcg
    11341 ccggaggaga accggcgcgc gatcgcggag ggcttcaagg cgctcgacca gttcaacaac
    11401 gtaacgctgc gcggcgccgc acgggaagtg ctcgatcaac tggagcgcga gcaacggctg
    11461 ggcgtggtgg tgcttggacg gccctaccac aacgatcccg gcgtaaacca cgagatcctc
    11521 gaggagttcc agaagctggg ctatcccgtg ttcacgcagg actcgctgcc gatcgacgac
    11581 gacatggtgt ggcgtctgtt cggagacgag gttcgtgcgg gcgacgtccc gcatccgatg
    11641 gcgatcacgg acgtgtggaa gaacgcttac agcgagaaca cgtcgcgcaa ggtatgggcg
    11701 gcgaaatacg tagcgcgcca tccgagccta gtggcgctgg agctatcgag cttcaagtgc
    11761 ggccacgacg caccgatcta tacggtggtg gaggaaatcg tcgagcattc cggcacgccg
    11821 tacttctgct tcaaggacat cgacgagaac aagccgacgg gatcgatcaa gatccgtgtc
    11881 gagacgatcg gctacttcct gaagcgatac cgcgaggaca tgatcgaagc ggcgactgcg
    11941 aaagactcga tcgacgcgcg actggccgag tacgagaaga agttgcgcct tcaactgctc
    12001 aaggagaagc tcgccgcgct cgaacgccag gaggagtacg tcggaggcgc catgccccag
    12061 attcacgttt cggtaggcgc cttggccgca tcgcggcccc aggaacccgc ccacatctcg
    12121 ggggattgaa ggggaggcgt tttatagctc tcctgactct gtcagccggc tggccgtgtt
    12181 ccgcggcggc ggacggccgc tatgctaaaa tcaccttaga ggggttgtag cctcccgcct
    12241 tcgaacttga tttccccgaa cgaaccaccg atcccagatc ccgagaatcc ggcagatccc
    12301 cagttccatc gccggtacga agcaatcgcc gccggagtcg tggtccatag ccgaagcggc
    12361 gcgatcatcc atgccaacaa cgccgcctgt cgtatcctcg cagtgaccct cgatcagatg
    12421 ctcgggaaaa cgtctatgga ccccgactgg caggcggtcc gcgaagacgg gtcgcctttc
    12481 ccgggagaag agcatccggc gatggtcact ctgcggactg gtcagtccct acagggcgta
    12541 gtcatggggc tctacgtggg aaatccgccg gcgttccgtt ggatctccat tgactcggcg
    12601 ccgatcaacg aaccaggaac cggggagttg accgaggtca tgacgacgtt cgtcgacatt
    12661 acggggcgca agcacgacca ggagatcctt cgccgaagcc agcaacgatt tcgcgatctc
    12721 gttgaaacaa cgagcgattg ggtctgggaa gtggacgaga acctgcgata cacctacgcc
    12781 agcccaaaag tgcgggacat cctcggatac gagcccgagg aggtgctggg ccggctcccc
    12841 atggatctca tggctcccga cgaagccgag cggctgtccg cgatgatcgc cgacacgatt
    12901 gcgaagcaag ctccgtttgt ggccctcgaa aatacaaacg tccatcggga cggtcacctg
    12961 gtcgtcctgg agacgaacgg aataccattt ttcgatgcac agggaacatt ccggggctac
    13021 cgcggaatcg atcgggaggt gtcagagcgc aagcgcgcgg agcaagaact ccgtcgcgag
    13081 cgggacttca gctccgcgat tgtcgacacc gtgggcagcc tggtcgtagt gctcgaccga
    13141 gtgggccgga tcgtgcgatt taatcgcgcc tgcgaacaat gcacgggtta ctcggcggag
    13201 gaagtcacgg gcaagccgtt ttgggatttg ttcctgacgt ccgaggagta cgagggcatt
    13261 cgggatgcgt tcctcagcat catgtcaatg agcctcgttc ccgacaggcc tgagttccca
    13321 agccgccggg tgaattactg gctcacgcga tccggagagc gtcgcttgat tgaatggtcc
    13381 aacacggtga ttaccggccg tgatggctct gtcgagtacg tcatcggaac cggaatcgat
    13441 attaccgacc agcggcaact cgaagaacaa ttccgccagg cacagaagat ggaggcggtc
    13501 ggaaacctgg cgggtggagt cgcgcacgac ttcaacaacc ttcttaccgt gatcaacggt
    13561 tacagccagt tggtgctgga tcagctcgga gacgattccc ccctgtccaa ggaggtcctc
    13621 gagatccgga atgccggcga gcgggcgacc acactcgtcc ggcagcttct cgcgttcagc
    13681 cggaagcaga tggcgcagcc tcgggttctc gatcttaacg cggtggtggc gggcgtcagc
    13741 acgatgctgc ggcggctcat cggcgagaac gtcgagcttg ttacggacct cgatcccgcg
    13801 ctgccgcccg tgaaggccga tccaggccag gtcgagcaga ttgtcatgaa cctggccgtg
    13861 aacgccgttg acgccatgcc gctgggcggc aaactcacgg tggaaacgag gaccgccgat
    13921 ctagacgggg cgtacgcagc acggcacctt ggcgtacagc cgggccgcta cgcgctgctg
    13981 gccgtaagcg atacgggtca cggcatcgac tccgaaatcc gcccacacct gttcgagccg
    14041 ttctttacta caaaggaagt cggccgtggc acgggtctgg gcctgtcgac cgtgtacggc
    14101 atcgtcaaac agaacgacgg gcacgtctgg gcttatagcg agcagggtat cggggcgaca
    14161 ttcaaagtgt acttgccggc cgcggaaggg ccggtcgcgc ctgtcagtcc gtcattggcc
    14221 gccgctgaac cggaaagtgg gaccgaaacc atccttctgg tggaggacga acccgcgctg
    14281 cgcgccatga cgaaggaggt tctgcgccgg cacgggtact cggtgctgga ggcatccaat
    14341 gcggccgagg cgttgcgcgt ggccgatgag aagggctgcc gcattgatct gctgctgact
    14401 gacgttgtca tgccaggcat gaacggccgg gaactggctt cacgtctggc cgagcgctgc
    14461 aacgggatta aggtgctctt cgtctccgga tacacagaca gcgccgtgat ccggcacggc
    14521 cttctgagcg catccgctga gttcctcgag aagccttttc cgcccagcgt gctgttgcgc
    14581 cgaatccggc agttgctgga tgctccaggt gcgtccgact agcgatcgtc actcgattga
    14641 gaacgagcga aactacgacg ctgatcacag ccagcgtagg gtggcgtaac gttcgcccgt
    14701 caagtgcaga aagagccctt cgagcgtgcg aaactccccg ccatcgaccg caatcggcat
    14761 gtcgcgagcc agagctgtga tatcgccctc ccacaccagg tctcccgggc tccggatgat
    14821 cgccacgcgg tcgcacagcc gctccacggt ctcaagcacg tgcgtcgtca tgaacacggt
    14881 gcggccctgc gccgtcaatt gccgcagcca ctgcttcatc aacgccacac cggccgggtc
    14941 gatggattca aacggctcat ccaggaagag gttcaccgcg gcagcaaacg ctacacgctt
    15001 gcgcatgccg gcgctgaatt gcgccagcgg tttccttccc tctcctgaaa gttccaaggc
    15061 ctccaaccgt tcggtcacgc gcacgcgcgt cgtcgcttca tctagcccat agatccgtgc
    15121 atgaaaggcc aggaactcgt gggcgtacag cgcttcaaac agccccagat tctccggcat
    15181 cactccgatc ctgcccttga gggagactgg gtacggccgg gtcgattcgc ccaggacgcg
    15241 catctccccg gaggttggat cgagcagccc agtcaagcag ccgatggtcg tgctcttgcc
    15301 ggagccatta ggccccagga atccgtagaa agacccggcc ggcaccttga gggagaggtc
    15361 ggcaaccgcg accaccaggt catattcttt acgcagatgc gaaatctcga tggcgctgtt
    15421 catgtaatta ggcggccgcc ttgttgctcg cgctctacca cggcgaggat ccgctcccgg
    15481 cgcgacacca acaccgctgg agccatgcaa agactgagag cgtagaaggc caccgcagcg
    15541 accatgaaaa acggagcgac ccaccagaat tgcagcgcgc gggccccagc ggccaccccc
    15601 agaaactggc tgccaacaag agaaaccact atcccgccgc ttcccaccag gttcgcagcg
    15661 agggaatagt cattgccgaa ccgtacctcg ccatccgtcc ggctcggcgt cagcatcgac
    15721 gtccagagcg ccagcgcctg gaaaaagcac aggccgccga cgccgttcgc ggccagcatg
    15781 gtcgccatgc gagcatcaat cggtacccgg gccagcagta cgcagaccgc gaagcccacc
    15841 ggcaaagtca gtgcgccgag gagcaacgga atcaggctca ccgccctcac gaccgccgtg
    15901 ggcgccagcg gcaaaaggaa ataacggcgg aagcccgagc cgtcaaatcc gaaggaattg
    15961 atcgccatgg cgaaggtcac cgagaagccg atgattgcca tcaggcccac tccaatgaag
    16021 aaatgcagca ggggcgcgtg gcgatccgga cgcatgatga ccagcatgag tggaaaggcg
    16081 ggagcgttaa tcagcaagct gaaacgcgcg cgtttggttc gccagtagta tttcagtgtc
    16141 ttcgcgacca gcggggctga ggagccgaag tgagcaacca gccgatcgac cggcccgtcc
    16201 cactccgccc gtgccgcagc caccgtttgg gaaggcacag gcaatcgttc gacacccgcg
    16261 atcgccgcgg ccattgccgc gcaccacacc agcagcaaca agaggccgat cgccgaacca
    16321 cccgccatca cccccgccgc acagaacgcc ggcgtgacct tcaccaccgg cagcagcgca
    16381 gccgacaggc tccgattcgc tccaatcgcg ggcgccgccg agaccgcaaa aaccagaatg
    16441 acgaccagca ccgccaggat cgccgggccg gctggtgtgg ccgtgatcca cccgatcatg
    16501 gcggcgagta gttgggcgaa caggtacgtg acgatcacca atagcagcgc cgctagcacg
    16561 gccaaccaga tcgagcccac ccccactgca tggaatccgg ccgcaagccc catgaacagt
    16621 gccagcagga tcatccacaa cgggtcgaga aaagccgtga tccgccgcgt cagaaagcga
    16681 tctcctgccg aaagcggata cctacgcaga acagcgtcgc agaatgccgg atttacgccg
    16741 attcccagaa ccaaagccgc cacaatcgcg atccccgaaa acccggcgag cactacgcgc
    16801 gccaccagct ctgccttgcc gagccggatc gacgccattg ccgctccgaa gccgcccagc
    16861 agcaggatcg cggcgacaaa gcacgccaac aggtatccga tcatgaatag cccgattttc
    16921 cctcgtcgca acctgacctg ggcccaaagc agccgataac ggaagccaag cagaactaga
    16981 aggacatctg gtcgcatata ggcctggatt ttcgttttag ccttctgccg caacaattgt
    17041 acatccaagc cgccgcaatt ccggacaagt ttgacttctt cgtttacgag cggctccaga
    17101 gtgtttctgg ccgatccgga gtgattctgg cacgcgcaac ctggcgcatt gctcagggct
    17161 ggtcgccccg tggctgacgt ctgccaggtg gccgggtcta ctcgtgcgtg ctgtaccgcc
    17221 gaacggacag cgccttcccg gtcgcctcat cggcctcgat gatgacggag tgcagctcgg
    17281 cgccggtctt ggccgcttcc atgcggatgg gcagggaagt gagaaaccgc cgaagtatga
    17341 tctccttctc gaccccgatg atcgagtcgt acggaccggt catgccgagg tcggtctgat
    17401 aggctgtccc gccgctcagg atcctcgtat ccgccgtggg aatgtgcgtg tgcgttccga
    17461 acactccggt gacgcggccg ttcagatacc agcccatcgc catcttctcg ctggtcatct
    17521 cggcgtgaaa atcgacaatc ttcactttca ccgaggagtc gagcgcggcc agcagttcgt
    17581 ccgcctttcg gaaggggcat gccgtcggcg gcatatgggt ccggccctgg aaattcatga
    17641 cagcgcactg gaccccgttc gtcgctttca ccaccacaga gccgctgcct ggcaggtcgt
    17701 cgggatagtt cgccggacgc agcagtttcg cttgccgggg caagtagtcg taaatctcgc
    17761 gcttatccca gatgtgattt cccgaggtca tcacttgaat gccgtaggaa aataactcgt
    17821 cggccaccgg cggggtaatg ccgaaaccgc cggcggcgtt ctccacgtta gcgatggtca
    17881 agtcaatctt ttctgctgcg atgatgcggg gcaggtgctg ggccaccatc cggcgtcccg
    17941 ccgacgcgaa aatgtcaccg ataaagagaa tcttcattcg cggtacgtcg tcaatgaggg
    18001 gagaaatgga gcgaaccacg gggccgtaac cgtaccctgc attgacccgc gtttgtaaac
    18061 ggggcggagt ccgccgcgcg ccttcaggct tctcccttac ggagcatgct caccggcgcg
    18121 taattcgagc cagacccctc tgcaattggt gttggatcaa ttttttggag tccggttacc
    18181 tgctttgtag aaacgctccg gatcgatgat agcagaaacc cagctcctgc cgcctaccga
    18241 aatgtgacac cctttatgtg ctggattgtg tactgaatgt tggcggcgtc caagccggca
    18301 gccgagaacc tggtcagctc gggagacggc tgcacgccca cagcaagaag accagacggc
    18361 agatgagcaa ccgggctgct gcccgaagga atcgccctca ggatcgactt tggaactgtg
    18421 aagcttcccg ttgccgcccg ctcggtgcaa acgaatgaac cgacgatgtt tccggagagc
    18481 gacgcaccaa cgatcgtcac cgcttgcttc gccggatcgc cgccgttcca ggtgagcgtc
    18541 aggtcctgcc cccggtcaac gtccgagatg ccatcccgat tcgtccacgc gaaggccggt
    18601 ggaattgtaa ctctcgcttc gaatggcccg atgtccgcgc ccccggcgcc gttgtccaac
    18661 ttatagtctc ccggaacgag aaagtccggc agcggctgcc catcgggctg ggatccaccg
    18721 agcgttgagg agtagattcc cttcgaaacg aggctgatct gctttgagcc ctgtggtccg
    18781 gtgacgttca cgaacggtcc ggcgtcgagc gtccgcgccg gtgcaggatc agacgagaac
    18841 cactgaactt catcctgcgc ccgaattgtg aacaccatgc aggcgccggg cacggaagag
    18901 acctgcactc cgcccagacc agccagcatt tcgagggcgc tacggcggaa gaacgacgca
    18961 gaagcctgat cgaccgaccc ctccgccgtc ccgatcttgc tgtacaccct gccaaggttg
    19021 atcgcgccaa acttcgcctc gcccgcggcc atcgttctct ctacatcggc tgccgccata
    19081 ccgccgggat ccgagcaggc cttcccggag gaggcaatcg acatcgtcgc aaaatgggtc
    19141 cacgacccgc gaaccatcac cgccacagga acgaaacagc cctcggcgcc ggagggcacc
    19201 tcgaacagga cctggtccaa ccccgcgcag cacccggaac gccccttgta actgaccttg
    19261 gcctcccggc cacccacgaa aacgcggacg tcatagggca gatcttcgat gcgcggcggc
    19321 ccggcgaact cgtcataact gaccgcgccg agtccggtac cccacaggaa cagcaattgc
    19381 cccggtttgg cggcctggtt gatcagattg acctggtagt tggtatcggt gatcaccgcc
    19441 gggccgctgc cgccgctgtt caacgtatac attccgaaac cgttctcgat gacgcggacg
    19501 gctatggggc tgctcgtctt accgttgtac gagacggtaa gctggccatc gcctgttggc
    19561 gccgccgagg gcatgatcgc cgagatctgc gtcgccggtc cgttgagcgg cacactcgcc
    19621 cgggagatcg gaacggcgtc gatggtggtg ttgccgatct tcacctggac tgtgatgccg
    19681 tccatctgcg ttcccagcgg aaatgccgat acctgcttga atccggacgg cccgaggtcc
    19741 ctcccgaaca cagtgaagat ggctcctctc ggaatgcccc agttcgccgt gccgggcagt
    19801 gagtagctcg ccgaatggag gaccgagtcg accacgggct gagcacacga gatgccaggg
    19861 gtgagcagac cagtgaagac cagcaagatg tacttcatct catatcctcc tagtccggtg
    19921 agcccaacac ataaaaccgg aaccggttcg actgtacgtc gttccacgtc agggttagat
    19981 cgagttcctt attaccctcg ccaacgtccg tatgcttcgg gatcaggatt gccgcctgcg
    20041 ccacccccac cagaccgggt acgagggtaa acgacttgag ttcaccactc gtgccctgct
    20101 gcggcgtcgc gggcttcgag agtatgatcc cttgcggctt ctcgattact tcgacctgag
    20161 ttgatggagc cggaacaccc gaactaacgg gtggattcgt ccgcccccct ccagtgaggt
    20221 acgcgatcat cgtgtcgccc gcatgcaaca ggttcgtggg agtgtttaga gtaccgtcct
    20281 gcttgtagac agcgagaatt cccggcgcca tgcctcgcgg tgtgacgccg aaggaccggc
    20341 ttgagacgcc gtccaccgcc accgtgagga acgtttgaga gtcgattgcc acggagtatg
    20401 gcaactgcgc cacgatcttg tcagcgctga ccgagaacaa aggaagcacc cggccaggca
    20461 cggcaacggt cgtgcctccg agctgcacag gtaatggcgt agcccggatt tcgatgggct
    20521 tgcccgcctc cgctgtctgt ttcgccagcc gttggccagt gatttcgata atgcaccccg
    20581 gcgcaaccgc tgtccctccc gaaccgaagt tcacgacttt cgttataacc ggccgttcga
    20641 tgggcgacgt ctgcgaaaag tccacttttg cgacgaaggc gttgcgcggg ccgcgcagcc
    20701 cgggctgaaa agactcggcg gtggccggga agtcggctga tcctgtctgc cccgtaagat
    20761 acgcattgcc ggccgaatcg agggcgacgg atgccgccgt gtccgggccg gaaccgccca
    20821 gaaacgttcc gtacagtagc gactttcccg ccggatcgag tttcatcaga aagccgtccg
    20881 actggcctcg aagtgccacc tgaaacgcgt cctccgatac gcgcaggccc gccgatgttg
    20941 tccggcccgc tactactacg tttccggatg aatccaccgc aaccgcacgg ccggcttcat
    21001 cattaccctc cgcaagccgg tttgagaaca cgattgcgga accagctgcg tttaacttag
    21061 tgacgaagga ccggccggcc accgtgaacg acccttccgt cgtagggaaa ttggccgagg
    21121 tcgtctggcc ggcaaggtac gtgtttccgg ccggatcccc ggcgattccg tagacttcgt
    21181 cgttgccgtc accgcccaag taggttgcgt agccaagact cgccccgccg gcgttcagct
    21241 tcacgacaaa cgtgtcccac cccccgccgc caaagcgcgt ctggtacgca cctgcggtgg
    21301 ctggaaaatc ctgggaggtt gtcatcccgg cgacgtagac gttgcccgaa gagtcgatcg
    21361 cgatgctgga tggatcgtca aagccgctgc ctccaagaaa cgtcgagtac agcagtgctg
    21421 tgccgttggg atcgactttg gtgatgaacg cgtcgcgctg gcctttgaag accgcctgca
    21481 gcgcgccggg cgtgacgggg aaattcgcgg aggccgttgc tccggtcacg taggcgctgc
    21541 cgctgccgtc gactgccacc gcggccgacg tctcataccc gttgccgccg aggctataac
    21601 agtacgcaac atcgcttccc atcggactca gcttgacaac gaacgccata ccgcttccgc
    21661 ggctccgttc cgccgggcag ggaagcgtcc catagccttt gacgccgacg acgtacgcgc
    21721 tcccggccgc gtccacggcg atgccggtgc cggtttcctt gtcactccct ccgaagtagg
    21781 tcgagtacac gatgttctta ccggccgggt ctatcttcgt tacaaataca tctgtgttgg
    21841 agttgccccc gggagtattc tggagcgctc cttcgttgac gggaaagtcc gtcgagatcg
    21901 tcgtccccgt cacgtaagca ttgccggtgg catccacgga aatggaattg ccggcctccg
    21961 catcggaaga accgccccca cccaggaaac tcgagaaggc caaggtggaa ccgagaggat
    22021 caagccggga ggcgaagctg tcgaagccgc ctctcgttaa acttccgccc cacgcgccca
    22081 gtgttaccgg gaaatcagct gaaattgtga ctccggttac ccaagcctcg ctcgccggcg
    22141 tcccggcggc gctgtaaatc cggtcttcgg atgcgccacc caggtaagtg aggtaccgca
    22201 attggaagtc ggcaaagttg agtgtcgcca cgaaaccgtc ggccgctccg cctccgaaac
    22261 tgctctggcg agccccccga gtgactggaa ggtcccgaga atcggtctct ccggcgacaa
    22321 ccagaatcct gcccgacggg tccacccaca cgctccgggc ggtatccgtt ccccctccac
    22381 ccaagaagac gcctcgtagt atctgcgtcc cctcggcgtt taggcggagc acgaaagcgt
    22441 cctgccctcc ggacaacgaa agaaagcgcc attcccgaag gatctcgcgc agataacgga
    22501 ccggatcgag caggccgccg gcaaacggcg gtggctcgat cgaattcacc accggaaaat
    22561 cggtcgaacc ggtcgatccg acgagaaatg cattgccggc cacgtcgaga gcgacaccgt
    22621 agaccgcatc cctccaaatc cctccgatca gcgttgagta agcgggcgat tccgatccgg
    22681 cggggaagcg gacggcaaaa ccctcgtcgg cttgggctgt ctttcgcaac gcacccgccg
    22741 ttaccgggaa atccgttgag gaagtcgtgc ccccgaccac ggcgttcccc gcttgatcga
    22801 ccgccaacga agttgcgtcg tcaatgtacc ctccgccgag aaacgtagcg tagagcagcg
    22861 ccgttccaga ggagttgatt cttgcgaaaa acgcatcggg cacgctgcga agcgtcttct
    22921 gaaaagcgcc cggcgtgacc ggaaaatcgg tcgagctcgt ctggccggtg acgaatgcgt
    22981 tcccagcgct gtcgattccg atggcagcgc ccgtgtcgat cgagttgccg ccgagcaggg
    23041 tggaatagac caaggccgtt cccgactggt tcagcttggc aacgaagcag tcaccgcctc
    23101 ccttcgggga cgtctggagt gctcctgttg ttgtcggaaa gcggttcgag gaagtggctc
    23161 cggtgacgta cgcgttgccc gatgggtcca cggcgatgcc tcgcacctcg tcgatcgagt
    23221 tcccaccgag gtatgtcgaa taaattcgcg ttgcgccgct tgggtcgaac ttggtcacga
    23281 aaccatcggt cgacccttgg ttagtcctgg cgattgcgcc atcggtgacc ggaaactgag
    23341 tggactgcgt ggaacctgcg acgtacacgt tgcccgacag atcgaccgca atcgcgcgcc
    23401 cgtagtcgtg gccgcccgtc accgcgccat caccgcccag gaacgtcgag taactgagga
    23461 tcgggtcgat gcggatgggc agcgacggat cgtgcgctcc gatctcgaag gccacgcgat
    23521 ggtccgctcg aagcgcgtag cgcacgggga tgtttcgccg gcctttgggc ccgtcctgga
    23581 aggcaagtgg tgcatgctgg cgcagcgcac cggcgctcgt atgcagcacg agatctccgc
    23641 catcgaggcg cagcgattcg gctccggaaa actcaacctg aacgtccttc gggttccggc
    23701 ccgggccgac gacgaggtcg tattcgacac gccgctggtt cccgtaatag acgagcgtga
    23761 ctcccggata gatgccccga caggcaacac gcccgtagtt cgggatgccg gtcttccagt
    23821 cgcgaggacc gcgtccggtc aggtagtggc tgcgcccggg tagggggtca agcgggtcca
    23881 ggcggcacgg tcccggagag ccagggaagc gcatccgcac attggcggtc gacccggcac
    23941 cggacaggga gaagaccgcc tcgccgccgg tgagaaacag aatgaatcct tcgcctcgcg
    24001 ccgcgtaact gacgcggggg tcgaattgcc ctcggttggc ctcaaaggac agcggcaggt
    24061 tgccgaagcc agcatttgcg cgcgagccga agcatagcgc gggcagaaac agcgccggga
    24121 gcaggagggc cggcgccacg aaccggagca ggcatgccat ggttcactcc gacagcagcc
    24181 gggttgtgaa gcccgtctcc tctctctaca tcggagtgcg gcaaaattca agatctaaag
    24241 accttaaaat tcatttttgc tatcaggacg tacaggacca acgctcggag accgtttccg
    24301 gtagtccgga ttgcctacgc ctcgcccgtc agcttgatcg gtggagtatt cttccactta
    24361 ccgcgtgtga agtccggaaa gtcgactggc gcgctgccgc gggccaccga gtgctccgag
    24421 agcggcgaaa tcacgctcca cgtcaccgcg tcgtatacgt tcatgtccgg ctcggtcccg
    24481 cgctggatcg cgcgcagcag ccttgcccac atgactggcg ccgtggcgcc cccgccatgc
    24541 ccgcgcgccc gcttgtactt cttttcgtcg agatttcgcc agagcgggtg ttcgaagtcg
    24601 gcgcggtagt tttcaagcgg ctcccactgg tgcgcctcgg ggctgcgccc ctcgatgtag
    24661 accttctgga tgtttccgga gaagtgcccc ttggttcctt cgaggcgcag ctccgccgtc
    24721 tgcgggtggg gtgtattggt gtcgaaatag agcaccgcga tctttccgct cgcggtcatc
    24781 atcagggtcg tgttgacgtc gccctgcttc atcttcatgc ccgcgtaggg gtgccggtcg
    24841 ccgtagtaga gcgacgcata ctcgttcagg cacaccgcct tagagctcat cgagacgatg
    24901 tattcgaact tgtctccgcg attgatgtcg agccaccacg ccatcgggcc gatcgggtgg
    24961 gtcggataga ggttgccgtt gcggtccacc gaatgctgca accgccaggg ctcccgctcg
    25021 gggtcgaact tcaccagccg caggtcgtga acatagccgc ccgtggcaca gaagagctgg
    25081 ccaaataagc ccttctgggc catctcgagc accgttagcc cgtgctggct gtagttggcc
    25141 tgctccagca tgacgcagtg cttgcccgtc ttctcggagg tctcgaccat ctcccagcac
    25201 tcgtccaggg tgagtgcggc gggtacttcg gtggccgcgt gcttacccgc tttcatcgcc
    25261 gcgatgcaca ccggcgcgtg ccattcccac gatgtggcgc acagcaccag gtcaaggtcc
    25321 gtgcgctcgc agaggcgcag aaagtccgtc ttcccattcc cgtatagcgt gggcgagggc
    25381 ttgcctgcct cctccaccca gcgtttggcg cggtacagat aatcctgatt gatgtcgcag
    25441 atcgccggaa cctcgaccat atcggtgccg agcaggaggt ccatgtggta cgagccgcgg
    25501 tcgccagagc ctacgatgcc gacgcggatc ggccgcgcgt cccgctgggc gccggcagcc
    25561 tggagtgcgg cggccagcac gttcattccg agcaattctc ttcgattccg ttcttccatg
    25621 gtggtctcct cccgctcaca cgatcaggtc cggatcgatg ggcgcccgga gcttccactt
    25681 gccccgcgta aagtcgggga tttccatcgg cttgctgcgg ttggcgacgg acgtctcggt
    25741 cagcggagtg atgacgctcc acgccgccgc gtcgtacacg tcgatatcga ccggccttcc
    25801 cgcgcgcagg ttttggatga gtcttgacat cacgaaaaag tcgatgccac cgtgcccgct
    25861 tcccttcgcc tcttcctcgc gggtccgcca gagcttcgag tcgtattgct tgcggtattc
    25921 ggccgtctcc tcccactcgt ggcggccgcg ccagttttcg tcgttggccc ggttgcttcg
    25981 gccctcgacg aatgccttgt ccatcaggct gttgaagacg cccttcgtac cctggatgcg
    26041 cagcaggtgc tccttcacgc gcggggtgtt ggtgttatgc cagagggtga ccgcccggcc
    26101 catttcggtt cggagcagcg tcacgctcgt gtcccctagc ttgtagtcgg tcttcgaacg
    26161 gtagtcgtcc gggccgaaat tcttgattgc gaattccttc atcgctccgg gccgcgagct
    26221 catcgacacc agcgatgtca gccggtcacc gcgattgatg tccaaccacc aggccatcgg
    26281 tcccatggga tgcgtcgggt acaggttgcc gttgcgccgg atcgtgtgat ccaaccgcca
    26341 gggctcgccg ttcgggacgg tgttgaactt caccgcacga atgtcgtgca gatacccggc
    26401 ttccgcatag agcggctcgc caagcacgcc ggcgcggatc atggtcagga tgagaagcgt
    26461 ctctcggtag tagcagtcgt tctcgagcag tgagcaaacc ttgccggttt tctccgaggt
    26521 ttccaccagc tcccaacact cctccaccgt gagcgccgcg gggacttcgg tggctgtgtc
    26581 cttgcccgcc ctcatcgcgg cgaccgcgat cggggtgtga tattgccatg gcgtggcgtt
    26641 aaccacaagg tccaggtcgt cgcgcccgca aaggcggagg aaatcggtct cgccgcgcga
    26701 gtatccctcc ggcttcggct ggcccgcgtc tacaacgaac ttctgcgcgc gcgcgaggtt
    26761 tgcctcgttg atgtcacaca gtgcgttcat cttgacgccc ggcagcttga gcagaatgcg
    26821 catcaggttc gtgccgcgcc ctccgacccc gacgaaaccg acacggattg tctccttcgg
    26881 ttgggccgag gccgcggggg ccagaacgcc ggccgcagca actccgaggc cggccttcaa
    26941 caggtcgcgc cggtcaattg gctggttcat gttcttttgc ccttcagatt gtcttcatgc
    27001 cgaacagttt tgcggcattg ttgtgataaa cctttttcaa gatcgcgtca ggaaggccga
    27061 tgccgtaaat ccgccaccgc ccctgcggcg gaacctcgct gggtgcgtag tcgaagtatt
    27121 cgtcctcggt ttcgaggaac cggaagtagg cctggaacat cggcggacgg agatcctgct
    27181 gcggaacgtt tttgccgttc ggcgtggcat cggtgccgaa catgatgcgg tccggaaact
    27241 cgccgaagaa cttccgcgag cgccgcggct gccggccgag ctcgccgata cgggcgccga
    27301 tttcgacgtg cagattcggg aacttcttta gccaggtgga aacctcgtcg agattctccg
    27361 gatggttggc cacgtggagt ccgatgaact gcgtcttcgg atgcctctcg atcacccggt
    27421 tgcgcgcggc gagaagctcc gccttcggcg gaaaatcttt accgtagaac gaccagtcgg
    27481 gatgattggc gagttcctcc cagcgttcgt tgaagcggtc gatcggcgtg aagaacgcgt
    27541 cgggatcgcc tgtgtggatc actaccggca gtttcagctc gcccgccgct tcccacatgg
    27601 gatcgaagcg tgggtcgtcc actttcacca gcgggccttc cttgcctctt tcgcgcaaat
    27661 acaggccgag ggtcttggcg attttcagac cgattgcgcc ggcatctttc ccgcgcttca
    27721 gctcgtccgc ctgccatttc ggatagtcag gttcctggac cttgtcccac gccggcgtaa
    27781 tgcagttggc atagtgtccc ttgtacttgc cgagcacttc gacattgcgc ttgaggtcgt
    27841 cgccgtaacc tccggagaca ttgatcagcg tctggaggtt gatctcgtcc atccactgca
    27901 tgatctggtc aagctgcttg acggcttccg gagatgggcc gccagactcc ggggcgcgcc
    27961 ggcggccaaa gacgctcgcc agatgcgtgt ggacgtcgat caccgggtac ttcgctttga
    28021 cgatcgggtg ctcgggaaca acgagcatgc tcttgggttg aaactcttcg agcacaagtt
    28081 tcgtcgacgc acttttctcc gctgttggag cgggcggctt cgaacacgaa gtcagtgtcg
    28141 cgccaagccc gccggcaaga aaagtgcgtc gcgaagacca catgaaaccg atttcattct
    28201 taggcacgga gccagcatat attcgcttcc accggatgtc aacacgagcg tctcacgagc
    28261 accagaactg ccacagtaac cgaattctct cgcttgacac gaactcgcca accagaatat
    28321 aatccgggca acaagagggg agactgggaa ccagctcctc tgggaaaatc tatggagacg
    28381 acgcagcccc cgcggggaag gaccgtgcca gcggtgaatc cgcctttgag aggcgcctca
    28441 ggcactgccg gcgagccgcc ccgcggcgca gtcccggccc tttcagaggc agaaaaaaag
    28501 aacgcgcgcc gccgggtcgt ttggaccgcg gtcatcgggt tcctttccgc cagcttcttg
    28561 atgttcctcc gcttcttctt tccgaggacc ctgttcgaac cgagcacgct attccgaatc
    28621 ggtttcccct cggatttcgg actcggcgtg gatgagcgct tcaagcagtc gcaccggatc
    28681 tgggtggtcc ggcagacgga caagctgttc gtcatctatg cgaagtgcac acacctcgga
    28741 tgcacgccga actggatcga gactgaaaac aagttcaagt gcccatgcca cggaagcgga
    28801 tacgacgtgg agggtaagaa tttcgaagga ccggcccccc gtccgatgga ccgcgcccat
    28861 gttgaggtcg atcccgaagg ccagattgtg gtggacacca gtcggctgta tcagtgcccg
    28921 ccaggggcgg tctgccagtt tgacacagat ccgaactcgt acttgatcgt ttcgtaatgg
    28981 accttatgcc atagcacgtt gtgagcgaaa agcggcagtc ggagcgaacg aaagaaatcg
    29041 ccatggaaca caggccagag gaatcgaggg gcgtcacgga actagccgag aaggcgcgga
    29101 aggagatcgc cggcctcgtc gaggacctga agcaacctca gaagactcag ctctggaagt
    29161 ccgtcttccg cgtcaagcac gagcgctcgg acacccggaa ccgttcgctg ggggttctgt
    29221 caaacgtctt tctccatttg catccagcca ggatcaaccg tgacgccgtc cgctacagct
    29281 acacctgggg catgggaggg atcagtttct atctgttcct cgtactgacc ttcaccggcg
    29341 tgatgttgat gttctactac caccccacaa aggttcaagc tttccgcgac gtcctctatt
    29401 tggagcacga cgtgccgttc ggtaagttgc tgcgaaacat gcaccgctgg gcggcgcacc
    29461 tgatgatcat caccgtctgg ctgcacatgt ttcgcgtggt tttgaccggg tcctacaaga
    29521 aaccccgcga gttcaactgg tgtgtcggag tcattctgct ggtactgaca ttgctcctct
    29581 cttttaccgg ctatctgttg ccggacgatc agcttggttt ctgggcggta acggttggga
    29641 cgaacatggc cagagcgact ccattgctcg ggagcgaagg gccgttcggc cccgagctgg
    29701 gtatgacgcc gttcaatgat gtgcgtttcg ccctgttggg gggctctatt gttgatgcga
    29761 acgcgctgct gcgagcgtac atctggcatt gcctcgcgat tccaatcatc gcagcggtgt
    29821 ttatgggcgt tcacttctgg cgtgttcgta aggatggggg catctccggt ccggcgcccg
    29881 tgatgctgga gtcggagatg aaagtggagc attcgcggta aggggcgggc cgcctcgagg
    29941 ctgggcaacc cgagggtagc ctttccgccg gcctgagcgc cgttctgcgg cgcggaggaa
    30001 gagcatggac tgggcgcagt tgtgggagat cgtatcgctc ccggataacg tgccgatcgt
    30061 ggcgctggcc atccttgtgc cgttctatgc atggtacggg ctgcggcaag ctcgggcgac
    30121 cgaccggctc acggttgcgc tcgaggccga tccgcaactg gccaaaaccc accaccgcaa
    30181 agttcatccc tacaagcccg gttgggcccg tgaggttcag gtctggccgt acctgctgcg
    30241 aatcgaattc ctggcggcgg tgattgtcac gctcgtgctg atgatctggt cgatcactct
    30301 caacgccccg ctcgaggagc cggccaaccc caatcacacg atgaacccgg cgaaagcccc
    30361 ctggtatttc gtgggactcc aggaaatgct ggtgtacttc gacccatggt ttgccggcgt
    30421 cgtactcccc acgttgatca tcatcggcct gatggtcttt ccctacatcg acacgaatcc
    30481 gctcggcatc ggctattaca cgattcgcca gcgtaaggtc gccctggcgg gattcggcgt
    30541 gggattcgtg atctggctgc tgctcatctt catcggcacg ttcatccggg ggcctgggtg
    30601 gatgtggttc tggccgggcc agacctggga ccacactcgt gtcatttacg aagtgaaccg
    30661 gaatctgccg gacctgttcg gcatttcaaa cccggtagtc ggcgccattc tcggaattct
    30721 agtcatcggc cttttttatg ctggctcagg ctggggcatt cataagctgg tcaccaggtc
    30781 gacgcttgat cggaaaatct acgagcgaac gagtcttctg caatatctga cgtttcaggt
    30841 ttttgcaatc acgatcctgg tgggtttgcc ggcgaagatg ctgttgcggc tattgtttcg
    30901 gatcaagtac gtgctcgtga cgccctggtt caacgtttag atggccggga cgcgaatgat
    30961 tcaggtggaa ctgctctgat gcccgacgag attaaccggg aagtgagaag cacggacgcc
    31021 gagccggatc cggtcgtcga ttcgtcgctg agtggaccgc tcctcatagc ggccgtcgta
    31081 ctctttctca cgatggtatg ggcgctctat gatgagctgt tctcccagcg cccctggaaa
    31141 cactaccagg ctcaattcgt gcggctctac acggcgcacc tccagaaagt aggtccgaga
    31201 gctgccgcgg ccgagaagga agcacgcagc tccccggagt ttcagaagat cgagcaactc
    31261 ctgaagaagg ccgaagactc ggctgctccc aaggtgaagg agattgaccg cgaagtggac
    31321 ggcattcggc gtcagttggc agccatcaag agcccgtttc aggatgtgcg cgccaggatc
    31381 gccgcattga cctacgaact ggaccatgca gccggcaagg ccgcgcggga ctccattcgg
    31441 cgggaagtgg acgagatccg caaaaggcag atgagcgtgg atctcccagc tgacggggcc
    31501 ggggccgcag taagcgcaca gaacctcagc ttcgtcgacc tcgaaaagag gttcaacgaa
    31561 ctccggaccc gcgaggccca gctcatttcc gagagggcca ccgtcaccaa tcaggcgcgg
    31621 gagctccgaa ggacgcgcaa cgagtatctg agcgagcaca tggttgggct cggtccccag
    31681 cagatcgaag gattgctgcg gaagatgcac ggcttccgga ttgagatcaa acagattcac
    31741 attgaggagg ccggtctggt ggaccggtgc gagtcctgcc acctcggcat tagtgagccg
    31801 ctgccgctca ccgccgccga catgggcggc aaccgggtgt tcgtcagcca tcccaaccgt
    31861 acgttgctca cgcttcacga tccgatccgg ttcggttgca ccccctgtca caacggcaac
    31921 gggctggcca ctgccagcgt cgaaggcgcg catgggaact acaagcactg gctctggccg
    31981 ctgttcccca aggagaacag cgaggccggc tgcctgcaat gccacttcac ggaccgcgtc
    32041 ctcgaccagg ccccggttct gactcgcggg cgcgatctct tcgagctgaa gggctgcgtc
    32101 ggatgccacc gtcatgagga gtttgaccgc gagtccgacg cgctcaccag catccgctcg
    32161 aacattcaga acctggaggt ccagcagaaa gaatcccgcc tcgaggccga ccgcgaagtc
    32221 cagcggggcg acacggccga atccaacgac caggcgcagc aacactacgc caaggcagag
    32281 cggctgcgcg tcacatcgag caacatggac gccagaatca aggagcttga cctgcaggcg
    32341 aaatctgtga tgcaggatct gaagaaggtc gctcccaacc tgaaggacgt tcggctgaag
    32401 ctccacaaaa actggatccc cgtgtggctg aagaatcctc acgcgtttcg gccggggacg
    32461 aaaatgccca gattccgcct ggtcgaccag gaggttcgcg cactgtcagc tttcatctgg
    32521 cagtccggct ggcagggacc cgctccggcg ccgcagccaa agggcgacgc cgcccacggc
    32581 aaagaactgt tcgagacgcg cgggtgcatg gcctgtcatt cgatgggtga gggaaacaat
    32641 cgggccggcg gcgagttcgc agccaacctg tcccgtctcg gcgagaaggc aaactacgac
    32701 tacatcgtcc gttgggtgca taatcccagg gaccgaacgc ggccctactg tccctacgag
    32761 aaacgggatc tcggcccaga ggactacgct cgccacggtc tcccgttctc cttcggcctc
    32821 gatcgctcga cgtgcccgaa cgacggccac gagttgcagg tccagaacat gacggtgatg
    32881 ccgagcctcc ggcttaccac ggaggaggcg cgtgacatcg ctacgtacct gacctccctc
    32941 aagcacgacg atgcggcgta tccgtcggac gtggctttta tggacgatcg ccgtctggcc
    33001 gaacagggac ggcagctcac cagccgctat ggatgcggca gttgccacga aatcgccgga
    33061 ctggaagacg cgccgcgcat cggcaccgaa ctcaccaagg agggtagcaa gcctctcgag
    33121 caactcgatt tcgcgctctt ggaacagaag gccaaagcgg agggttggta caaccacaag
    33181 ggctttttcg agcgtaagat caaggacccg gcgttcttcg acacgggccg cgagaaggcg
    33241 ctcgaggacc ggctgcgaat gccgaacttc gagttgtcgc cggaagactc ccgcgccctc
    33301 atcacgttcc tgttggggag tctggactcg cccttccgat ctgagttccg ctccatcccg
    33361 cagcagttcc gctatatccc gaccgaccag cagaaggacc tgcaggaagg ctggtggctg
    33421 gtcaagaagt acaactgcat gggctgtcac acggtccagg ccggccagca ctccgtgctg
    33481 agcgcgcttc ctcgttatca ggatccggac tggaaggaac agttgccccc ctcgctcgtg
    33541 caggaagggg cccgcgtgaa tcccgagtgg cttgcgcgct tcctcagcaa tccagccatg
    33601 accgagaagg acacgtccag gaacggcgtc cgcacgtatc tcaaggcccg gatgccgacg
    33661 ttcagcttct ctcccaacga aatccgcatc ctggtgcggt ttttcgaggc cgccgcggga
    33721 cagtcgagcc cttacatccc gtcgaagctc gaaccgctga ccgagccgga gcgccagatg
    33781 gcccgcgcgt tgttctcgtc gccgggcgcg ccgtgcctca ggtgccacct ggtcggcgag
    33841 ccacgtcatg accgctcggc tacggcgcca aacttcctcg tggcaaggga gcggctcaag
    33901 ccggcttgga ccgcccggtg gatgctcgat ccccagatga tcagtcccgg tacggccatg
    33961 ccgtcaggat tgttccggcg ggaggacgat cgttgggtct ccgccggccc cacgccgggg
    34021 atcttctcag gttatcgcgg agatcacgtc gatcttctcg tccgctatat gttccaactg
    34081 actccgcagg agcagagtgc cttaattcaa agactgccgg caacggcccg cactgaccgg
    34141 ccgccgccgg acgagacggc gacggtcgcg cattcccggt aattatgccg catgaaaagg
    34201 agaaagatga aaacgcaaca tcgatccgca acacagaggg ccggagcagc gatgatcgct
    34261 ctgacagcgg tcgcagcgct tgctctcatg cctggctgcg gcggaaagaa agagacaacc
    34321 cccgcggccg gggaacagcc ggccgccgga ggtgaagtct ggcgcccgac cggcgacgag
    34381 ggaacggtta ctggcaaggt ctccttcaag gggcagccgc cgaagttccc cgccatttcg
    34441 atggatgcgg acgccgtctg cgcggccaag cattccggtc ccgtgcaccc ggaggcggtg
    34501 atcgccaatc ccaatggaac tttgaagaat gtgttcgttc acgtgaagag cggcctcgag
    34561 ggcaagacct tcgccgtccc gacggaaccc gttgttcttg atcaggtggg ttgcctgtac
    34621 aaccctcacg tgctggggat acaggcgcgg caggacctca aggtggtgac gagcgacaac
    34681 accacgcaca acatccatcc gctgcctaag attaatcgcg aatggaacat ctcgcaaccg
    34741 ccccacgcgg atccgatcat caggtcattc agccgtccgg agatcagcgt ccctgtgaag
    34801 tgcaatcagc atccttggat gagagcgtac attcacgtcg tctctgatcc cttctttgcc
    34861 gtctccgacg accagggctc attcgagatc aaggggctgc cgccgggcaa ctacgagatc
    34921 gaagccctgc acgagcatta cggcgcgcag acgcaaaagg tcacggtcgc gcccaagggg
    34981 tcggcttcgg tcgaattctc atataacgct acgcaggcgt accgcgccgg ctctcttcgg
    35041 atgatgccgg ccctggtcat cccctgctgc ggaggtggag gaacagaacg tggcgcgagc
    35101 cactagcgca gagctcgccg ggcgggcctt gccgctcgaa ggccgcaagc tccttgcgta
    35161 tctggagctg accaagccgc gcgtaaccgt gatgatcttc ctggttacga tcgccgcctt
    35221 ccggctcggc tctccggcac aaaccgacgg ttggagatta ctatgcaccg tagccggcgt
    35281 cggactgctc gccggcggta tattctccct aaaccagtac atcgagcgcg acatcgacgg
    35341 cgtcatgcgc cgcacgggga tgcggccgct tccccggggg gagctcaagc cgcgcgaagc
    35401 gctccgtttt gggctggcgc tcacgatcct gggtgtcgcc atgctcgcgc tgctcgtcaa
    35461 cgcccttagc gccgtgctgg ccgtttgtac gctcgtcggg tacctgtttg tgtacacgcc
    35521 gctgaagacc aggacgcctt actgtaccct cgccggcgcc ttccctggag ccatgccgcc
    35581 gctgctcggc tgggctgccg cccgcggcga gatgggagtg gaagcttggg cgctgttcgc
    35641 cgtccttttc ttctggcagt ttccgcactt tcacgcgatc gcccggctgt atcgcgagga
    35701 ctatgagcgc gccggcgttc gcatgctgcc ggtgatcgag gcctctggca accggaccgg
    35761 ccggcagatc ctgctcaccg cgctgctgct ggttccggcg agcctggccc cggcgctcct
    35821 cggtctgact ggatccgtct acctcggcgg cgccgtcctt ctcagtcttt ggtttctgta
    35881 ccacggcgta cagtcgttcc gggtggcgtc cccgcggcaa gcgcagcgat tgctgggtgc
    35941 atcgattgtt tacctgcccg cgctgttcct gctgatggtg ctggacgcgc ggtgaccgga
    36001 taggaggcgt ttcatggcga tggtgttggc cctgttcttc tggctgttgg cggttgtcac
    36061 agccggaatc ttcataggcc gggtatggtg gctccccgag ctcgtctccg agcatggggc
    36121 cgccgtcgac cggcaactgg cactgactct cctcatcgcc gggatcgtct tcttcgccgc
    36181 tcaggtcgga ttgggatggt tcatctggcg ctaccgggcg cgcggcacgg gccgggcctc
    36241 gtactggcac gagagcccga agctcgagct cacctggacg ttgattaccg cggtcgtatt
    36301 tatcggcctc gccattcagg gcaaccgcgt ctgggctaag tatttcctcg acgacgctcc
    36361 gccggacgcg ctgacgatcg aagtgaccgc ccagcagttc gcctggaaca ttcgctatcc
    36421 cggtccggac ggcaagttcg gacgcaccga cccgaagctg atgaatgacg ctctggccaa
    36481 ctacatcggg cttgacccta acgacgcggc cggccgcgac gacatcctta cgcagaacct
    36541 catggctgtg ccggtgggcc ggcccgtgcg agtcatcgtt cgctccaagg acgtgacgca
    36601 cagcttcttt gtgccgcaac tgcgtgtgaa gcaggacgcg gtgccgggga tggccatagg
    36661 gattcacttt actgccacaa aggtcggcga atacgagatt gcctgcgccg aactctgcgg
    36721 catgcagcac tacaagatga ggggccggct cctggtcatg gattcggcga atttcgagaa
    36781 gtggctgaag cagcgagcgg gcgcatagga gcaaagcgcg atggcggacc atgaacaccg
    36841 gcatgaggca cccaagggct tcgtccggaa gtacattttc agtaccgatc acaaggtgat
    36901 cggcatccag tacctgatgc tggcactgtt ttccgtctgg gtcggcatga tcctgtcggt
    36961 cttcatgcgc ctccgcctcg cctggccgga ggagaaatgg ccgctcctcc agcgcgtgtt
    37021 ccaggagggc ttcgccggag gtcccatgac tccggagttc tacctctcaa tgctcaccat
    37081 gcacggcacc atcatggtgt tcttcgtgct tacgacagcg ccgctgtcgg gcttcggcaa
    37141 ttactttttg ccaatccaga tcggcgcgcg ggacatggcc tttccagtgc tgaacatgct
    37201 ctcgttctgg gtcacgttcg tggcgctggt tgtcatgatg ttggcgttgt tcgcaaccgg
    37261 cggcgccccg atttccggct ggacggctta cgctccgctg agcgcgttgg gcgaggtggc
    37321 cggccccggc ctcggccttg gacaaacgct ctgggtcata agcatcgcca tcttctgcgg
    37381 agcgtctctg ctgacagcgc tgaacttcct tacaaccacc ctgaaccttc gggcgcgcgg
    37441 catgtcgctg atgcggatgc ccctgacagt ctgggcgtgg tttacgacgg cgattctggc
    37501 tctgcttgcc ttcccggtcc tgctgtcagc cggcatcctg ctcctcatgg accgcctcgg
    37561 cggaacaagt ttcttcattc ccggaggcct cgtcgtcagc gaccagatga ttgcgcacca
    37621 gggtgggtct ccgctgctct ggcagcatct gttctggttc ttcggccacc ccgaggtgta
    37681 catcgcgatc cttcccggca tgggagtcac gtcgcacatc ctctcgacgt tttcccggaa
    37741 acccgtgttc gcttacaagg cgatggttgc ggcgatcttt gccatcggct ttatcagcta
    37801 cctcgtctgg gggcaccaca tgttcatgag cgggatgagc ccctactcca gcatggtgtt
    37861 ttccatcacc actctggctg tgggagtgcc gtcggcgata aagacgttca actggctggc
    37921 gaccttgtgg ggcggacgga tccgcttcac aacgccgatg ctgtttgctc tcggcttcgt
    37981 ctcgctattc gtgtcgggag gagttacggg catgttactg gcccagcccg ccgtggacac
    38041 tttcctgcac gatacttact tcgtagtcgc ccactttcac atcatcatgg gcgtcgcggc
    38101 gatcttcggc atctttgccg ggatgtacta ctggttcccg aagatgttcg gccggatgat
    38161 gagcgaggga atgggtaaag tccacttctg gctcacgatt gttggtgtct acgcgatctt
    38221 catgccgatg cacttcctcg gtgtggaagg cgccccgcgt cgttacgccc agttgaccga
    38281 gtttcagtac ttgcggcatc ttcagccgct gcacctcttc attacagtgg ctgcttttgc
    38341 gaccgtggcc gcgcaactga tcttcctctt caactttttc tggagcatga aaaagggcag
    38401 gaccgcctcg gagaatccat gggaatcgac gtcgctcgag tggaccgtac cctcgccgcc
    38461 gccgcatgac aacttcggcg atgtcgaacc ggtcgttcac cacgggccgt acgagtacag
    38521 cgttccaggg gcagcgaagg actacgtcct gcagaccgcc ccggcagagg aggtcacccg
    38581 ctaaacggct ggatatggct accgcaagca aagcgacgaa acgggccgga gggtggagcg
    38641 gtctgggcga aggcttcggc tggaacggcg atggcagcgg gcgtgagcgt cccatgggaa
    38701 ctcctccccc agatgtgcac cgcctgggca tgtggtttgc cctgtgcgcc atcaccatga
    38761 tgttcatcgg actcaccagc gcgtatgtcg tccgccaggg aattgacccg gagtggcgtc
    38821 cgatccacat gccggcgttg ttgtggctga acactgccgt gttagccatc agcagcttca
    38881 cgatagaatt ggcccgcgcc gcccaggggg tgggcgttga cgggcgccgg gtgtggcttc
    38941 ggacgatccc cgtcggtggg ttcacgagtt gggtcgcggc aacgctggtc ctcggtcttg
    39001 cgtttctggg agggcaggcg gtggcatgga ggcaggtcgc ggggcagggc atctacctgg
    39061 ctacttatcc gcacagttcg tttttctacc tgctgaccgg tttgcatggg ctgcacgtcg
    39121 ctgggggcgt cgccgcgctg gcatggctct ttgcccgcgg ggcgaacttc tctcctctgc
    39181 gccgccgccg ctggactgaa gcgaccgctc tgtactggca ttttgtggct ggcttgtgga
    39241 tctacctgct ggccttgctt ttcgtccgga gatgatgccg gcatgtcgtg aggaggtgat
    39301 gtgggatgac tgaacgagct gttcgagcac aaagtgcaac catctgggac ggcggcgtcc
    39361 atccgttcgc tgtgagtcat aagaagctcg gcatgtggct gttcatcgcc tcggactcgc
    39421 tgactttttc cgccctgctc gttgcataca gctacgtgcg gctagccacc gagcattggc
    39481 ccacgccgtt ccatctgtgg ccttccatct caatggcgac gatcatgacc ttctgtctgc
    39541 tgtcctcgag cgtcacgatg gtgatgggcg ttgccgcctc gaaacgaggg gagaaggcgc
    39601 gggcgattcg ttaccttctt ctgacgatgc ttggtggtac ggccttcgtt gtcctgcacc
    39661 ttaatgaatg gctgggcctc atccgggagg gtgtcaggtt gtggagcaac ccgtggaacg
    39721 tgccgctatt cggcgcgacg tttttcacgc tcaccgggct gcacatggtg cacgtcgcca
    39781 ccggcttgat ttatcttggc gtgatcgccg ccggtgtctc caaaggccgc ttcggccacg
    39841 aagaggtgga agtcagcggg ctctactggc acttcgtcga tctggtctgg atgttcattt
    39901 ttcctttggt ctatttgctg tcggtgagcg cagcatgagt gctgcgtttt tgggggacgt
    39961 atgagcatcg gaatcgaaga gacgcacgct ccgcacggtg cgagcctcta ttactgggtc
    40021 tggatctacc ttctggcgat aacggcgctg gaggtcttcc tggcttacga gcacctgttc
    40081 tcgacccagg tcatgttggc cgtcctcatg cttctgtccg tcgtcaaggc gggcttgatc
    40141 atggcgtact tcatgcatct gcgcttcgag aaagccgttt tcgtcttatg tctcgttccg
    40201 gctgtcgtcg ttgtcatctc gttgctggcc gccttcttcc cggacagcta ccggctgctg
    40261 gagcttaggg tgcgatgatg acccggcgcc tgtggcagtc gatcgtccaa acgctggtac
    40321 gagtcattgt gctcgtggtt gcgcagtcgt ctctgttcgg ccagtgctcc atgtgcaggg
    40381 cggccgccgc ggcgcaggga ccggaagccg cgaaagctct gaatgaggcc atcctgattc
    40441 tgctgcttcc cgcggtcgct ctgttctgcg gcgccttcat cctggcgatg cggggaaacc
    40501 gttcgtcgga cccggacgag tcccggcggg aaggagcgcc gggatcgtgg cctcaatgac
    40561 ccttactatg tggcagcagg ccatcatcta cttgctctgc ctgatcgtcg ctgtcggctc
    40621 gatcgggacc gccctttggg cgcttctcac tggccaggtt gggaccgagg gcatggatgg
    40681 tctctttctg atcgcggtct ccctcttatt tgcgtttacc ttctcgctca ccccgatcca
    40741 ggctttgcgc cggggcttcc tgcgggactt gcttgcaagc aggaaagcgg ctgccaagcc
    40801 acgggaggaa cgggacgcgg gcgccctgcc cgctgacgaa gctcacgaaa ggaccccatc
    40861 gccttgactt cggagcggcg gcggtggctc gtgctcgcgg cgctggccgt gctcgccgta
    40921 atcgtaccgt tcaccgtgtg gcgcgagacg tggttcggcc ggccactctc ggacgacgag
    40981 atcggacgat atctcaactc cacggatcgc gcacgcaagg ttcagcacgc tctgtcccag
    41041 gtctccgaac gcatcgtccg aggcgaccgg ggcgccgagc ggtggtaccc ccaggtcgtc
    41101 tcgctggccg gccatccgct cgcccctgtc cgcgccaccg ccgcctgggt catgggccag
    41161 gataaccggt cggacgcctt ccaccgggcg ctgctggagc gtcttcggga ttcggacctg
    41221 atggtccgcc gaaacgccgc gctgtcactc gtgcggttcg gcgatgggag cggaaaggcg
    41281 gagatccttg ccatgctcca tccgttcacg gtccgcgctc cgaaagccgg gaaggtctcc
    41341 gtcggtctgg agagcggccg ggagatcagc cagggagcct tactggcgcg catcggcaag
    41401 gaaagcgcca gcccggtcga ggtccgcgct ccgttttcgg gccgcctcgg ggtcgtactg
    41461 attccggacg gaacgcctgt agcggaaggt gagcctctca tgatcatcaa cccggcgtcg
    41521 gaatacgtct gggaagcgct gcgggcgcta tatcttgtgg gacagccgga ggaccttcct
    41581 gacgtggagc gctgcactcg ggtcatcgcg gagatgcctg agtccgtgcg ccgtcaggcc
    41641 gaatacactg cccgcgctat acgtacgcgc tcggaacgga gttccattcg ctgacgggcc
    41701 ggtccgggac cgacggcagt tcgatgccgt gcccgccttc ccattcctcg actgctacca
    41761 tgtcgatggc gtcccacgga cagccatcca ggaaagttcc gtccgggccc ttgctggtgc
    41821 acttggcaca gccgatgcag gtggccttat ccacttcgat ccggccgaag ggttcatggt
    41881 ccgggtccgg tacccagaac atacacgctt ccacgggaca gtacgctaca cagacgggcg
    41941 cccccgcgca acccgtgcag ttatcgttga ttacggctaa ctgcttgggt ttcttctttc
    42001 gcaggtccgt tcgcccatgc gacatcggct cgagttttcc aatgactatc gccagttcag
    42061 gtcccattgc gtagtcccca gggagtgcct acctcaattc taatgcagga cgcgctccgg
    42121 cttccagcgg aatcctcaac actagatttt ttcattgact gtcattaagg gttcgtagta
    42181 ttcttctcac aacttcaggt ctgcatatcg gttttttgga ggtattggat gtacaagggc
    42241 attgcacacg ttctcttggg gatggcgatc gtgctatctt tcgccactct agtcatcggt
    42301 caggaaacca ccggagcaat cctcggcgtc gtgcgggaca acacggacgc agtcgtcccg
    42361 gcagcgaagg tcaccgctat cagcgaggat accggtgcgc agttcgccac ggaaaccgac
    42421 gcgaccggca gctatcagtt cccgctgctg cgcgctggcc gctatcgcat ctccgtcgag
    42481 caccccggat tccagcgaat gcaacgcgac ggggtgattg tgaacacaac cgagcgcgtc
    42541 cgcgtggaca tcacgctgaa ggtgggcgtg gttacagaga cggtcacggt gaccggcgag
    42601 gtcccgcttc tgcaaagcgc ccaggtgacg atgggacacg tcgtcgaaca gcggacgatt
    42661 acgtcgattc cgctcgccac ccgaaacttc acgcaaatcc tcggcacctc agcgggcgtg
    42721 atcggagcca tctacaacgc cgaccaaccc ggaaccggca gcgacagcgt cagcgtgaac
    42781 ggcatgcgcc gtggcagcaa taacctgctt gtcgacggcg cgcaaacgac caatccgctg
    42841 aacatggcgc ccgacggcga cggcacgccg agcatcgagt tcttgagcga gttcaaggta
    42901 ctgaccagcc tgtatagtgc cgagtacggg cgcaacgccg gttctgtcat caacgtgacg
    42961 acgcgcagtg gcaccaacga gttccacggc tcagtctacg agtttttccg caacaccaag
    43021 ctgaacgccc ggccgttctt caacccgacg cgcgggcaga acaaccagaa ccagttcggc
    43081 gggcacgccg ggggcaagat catccgcgac cggacgttct tcttcgccgg ctgggaaagc
    43141 tcccgccagg tgaacgcgaa ctccgggtca gcgacgctcc gtaccatcgt ccccacggcg
    43201 gcccagcgcg ccgggaactt cggctccaag acgattacag atccgactac tggcataccg
    43261 ttccagaaca acacgattcc cgctgaccgc ctcaacccga tttcgctcaa gcttcaggag
    43321 aagtacatcc caacgccgaa tttttcctcc ggcgcgacga acttcttcgc ggcgcgtggg
    43381 cttccgaccg acctgaacca gtacacggtg cgcatcgacc accgtttcag cgagaaggat
    43441 acgatcttcg ggcgctggtt cgatagccgt cagaaggact tttcgccgtt tacgtggggc
    43501 ttccccgggt tcggcaacta cgcaaaccgc aagaagtact cgttgaacgt cagtgagacg
    43561 cacctgtttt ctccgacgct gatcatggaa acccggttcg cctacgatca gaccgatcag
    43621 tacacggtgc cggaaaacct cgactccttc gatagcgtcg gcttaaaacc tctctcggtg
    43681 acgcgcacgg attacggact gccgcagttc aacatcaacg actaccagac attcggcaac
    43741 tatcagaatt ggtccgacta catcaagcgg ttcaccggca gcgccagttt tacctggctt
    43801 cgctcctcgc acagtgtcaa gttcgggtac gaaagcgtca acgctctgta taacccgaag
    43861 aacacgctgg acagccgcgg ccgcttcttc tacaccggcg aggccactgg cgactcatac
    43921 gccgacttcc tgctgagcat cactcgttcg aagagcttcg gcgccggcgc cggcgagttg
    43981 aagatgcggg actcggtgag cagcgtctat atcagcgatg agtggaaggt caaccaggac
    44041 ctcaccctca ctctcggttt gcggcacgag gctcacttcc acccagccgc ctacaacctc
    44101 ggcatgacga actggtatcc ggatcggtac aagggcgtcg gctcgctcga ggcgtccggg
    44161 gtcgtccagg gcggcgttgg cggtattcct caaagcacga tcaacggcga ctggaacaac
    44221 ttcatgccac gcgtgggcgc cgcctggcgg ctcaccgaca gttgggtgat tcgcgcgggc
    44281 gccggcctct atttcgacca gagatccggc cagatcgccc agcaggcgtt ctccaacccg
    44341 ccgactttca agtcaatcgc accggattgc agcgtcgcgg gcagcggctg caccttgaag
    44401 gaacccgaca acttcacgtt ccttgacccg cagtacgacc cgcaattcat cccgttcccg
    44461 accaaagtga ccgattctct ggcttggcgc ggcatcgaac gcgataccag gaccgacaac
    44521 gcttggcagt ggaacttcaa catccagcgt cagcttccaa agggcttcct tgttgagggt
    44581 gcctacgtcg gcaccaaggg tacccacctg atggcgcgga ggaatgtcaa cccgctcgtt
    44641 cccgccgggt tcgatccgaa taaccccaag accggaaccc tgaccaggag gtacccgggt
    44701 ttcgccgaca tcttgatcac cgcccaaaac ggcgactcga tctaccactc ggctcagttc
    44761 acggtgaaac gccgcatcgc taccggaacg atgcagatcg cctacacgat cgccaagacc
    44821 ttgagcaacg gcgcggaagg aaaccgctac ttcaccagca tctatgacac gccgtggtgg
    44881 gacttcagcc gggccagggg gcccgccaat tacgaccgtc cgcagcgctt caccgcggtc
    44941 ttcacgcaag acttgcccaa ggttggccag agcgcggttg cgaggcacgt gttgcacaac
    45001 tggtcgttca acggattcct ggtggtgcag tccggcactc cgcttacggt gactaaccgc
    45061 gacagcggac gaaacctcgg cggatccgcg caggagacgg cccgggcact cttctcaaac
    45121 gtagcggccg accagccgct cgtcaactcc ggctcgacga aggacaacct gaagaactac
    45181 atcaacaagg cggcgtggag caaagctgcg gtcgggactg tcggcaactc ggggcgcggg
    45241 atgtttcgtg gccccggaca gtggaatctt gatttctcga tgttcaagga catacctctt
    45301 acggagcgat tcaagctgca cttccgaagc gagttcttta acctcctgaa ccacgcaaat
    45361 ttcggcgatc caacgaccaa cctcgattcg agcagcttcg gccagatcag cagcactgcg
    45421 gtgaatgcgc ggctggtcca gtttgcctta cgattgaatt tctgaagctt cttcttctgt
    45481 ttctcgcggg cgcgctgctt ggcggcgcgc ccatcttcgc ccaagggatg gggagcggtg
    45541 tgaagtcgca ggcccgcccc tctcccaagg ccaacgtcaa cgttcccacg ccgcgcctcg
    45601 aattccgcga cattacctcc tcaactggga tcaccgcgtt aaacgtctat ggaggcgaca
    45661 aaaccaaacg ttacatcctc gagatgaacg gcaatggcgc cgcgatcttt gattacgaca
    45721 acgatggctg gagggacgtg tttcttccga gcggcacgcg gctcgatccg atcgaaggcg
    45781 cggagaagcc cacgaaccgg ctctaccgta acaagggcgg cggcgcgttt gaagacgtga
    45841 ccgaagccgc cgggctcatc cgaaccggat gggggcaggg cgcctgcgcc ggcgacttcg
    45901 ataatgacgg tcacaccgac ctgcacgtca cctactacgg ccagaacctg ctctaccgga
    45961 accgcgggga cggccggttc gacgacgtga cggagaaggc cggcctcgcc gccaaatggg
    46021 cacggtggtc tacaaactgc tcgttctttg actacgaccg ggacggactg ctagacctgt
    46081 ttgttacttc gtacctggcg gtcgatctga aagagatcca gccgcccgga agcacgccct
    46141 attgcgcgtg gcgcgggctc gccgtattct gcggcccgcg cgggttcccc accggcgaga
    46201 acctgctctt ccataacgag ggtggcggcc gcttccgcga cgtcacgcgg cgcgccggaa
    46261 tcgctttgcc ggatctgcac tacaacctcg gggccgtaac tgccgacttc aataacgacg
    46321 gctggcccga cattcacgtc gcgagcgact caagccccgg catactctac cgtaacaaca
    46381 aagacggcac gtttaccgac gtcgccgtgc tcgccggcgt cgcttatggc gacgccggac
    46441 aggagatggg cagcatgggt acctccgcgg gcgattacga caacgacggt ttgatcgata
    46501 tcgtaaagac gaacttcatg gacgagacgc ccactcttta ccgcaaccgc ggcaacctgt
    46561 ttttcgatga cgcgacgtac atggccggtc tcggcgttaa tacgaagttc gtcggctggg
    46621 gcgtcgactt cctcgacgtg gaccaggatg gttggaagga catcttcatg tcccacggcc
    46681 atatctaccc agagttgctc gcctccgatg tgagcgagcc gtaccggcag cggaaggcgc
    46741 tcttctggaa cgtccgcaac ggagcattcc gcgacgccac ggagaaggcg ggcgacgcac
    46801 tcaccacgcc gaacgtctcg cgcggattcg ccgccgggga cctcgacggc gacggaacgc
    46861 cggagatcgt cgtcgtcaac atgaacgagc gcccggctgt gctgaagaac accggagagc
    46921 gcgggaacgc catcctcgtc gagttgatcg gaacgaagtc gaaccgcagc gccatcggcg
    46981 cccgcatcaa ggtaacagcc ggcggactcc agcagattga cgaggtccgc agtggcggcg
    47041 cctacgcctc gcagagggac ttccggctgc acttcggtct cgccgctgcc gacaaagcgg
    47101 atgtacgggt tcagtggccg agcggcgctg tggaagagat caagggcgcc acggcgaacc
    47161 aatggatcac gatccgtgag ggcgagggag tcatcaagcg gacgccgttt cgctgaggcc
    47221 ggggaggatc agcctttccg cacttcgtaa gcgagccgcg gcatttcccg ccgcttccgg
    47281 tcggtctcct cgcggtcccg cagaacaagc tggtggtgtt tctggcggtg ttccgcggcg
    47341 cgggctgtgt cctccaacct agagtaaatc tgcgcgaggc tgtaatgcgc cgcggcgtga
    47401 tcggggttaa gcttcagcgt ctgctcgagc gccttcgccg cttcgtcgaa ccggtcctgt
    47461 gccttgagga cagtgtgcaa ctcgaaccat gccggccaga agccggagtc ggttgcgatg
    47521 gccctcctca aaagcgcttc ggtttccgcg gtggcgccgt cccgggtggc gacgcccatc
    47581 gccagcagat agtgagccac cgggtttttc ggatggcgct ctgcgaatgt gcgaaacttg
    47641 ccgaccacaa ggtccaggcg gttgcccgcg tctggcaaca gagattcgag cgcggcgtaa
    47701 ctgagcgccg agtcgggctc catctcggtg acgcgtagaa actcgtccag ggcgcgttcg
    47761 gtgttgccga tggcgtagca cgccagaccg agcatgcgcc gcagttcgac gtctcccgga
    47821 aaacgggtca tggcctgatc gaggagcatg cgtgcgggct ctggggtgtt gtgatcgagg
    47881 aaaagctgcg ccaggtcggc gacataggcc ggctgggcgg gttccaggcg aatggcctcc
    47941 tgaagcgccg cgacggcttt cggcggatcg cgcttcagcc ggtaggcctt gccgagtatg
    48001 ctctgcgcgg gcgccgacgg cccggccgct gcgacctccc ctgcaagctg aatcgccgcc
    48061 tcggcctcat tcgcgcgcag gtgatattcg gccgtgcgga gaagggcgag ccggtctgtc
    48121 ggattggcgc gcgcccagcg ggtttcctgt tcggcggctt tggaaaactc cccggcctgg
    48181 ctgtagaaca tcgccagcgc gtgggcgacc accggttcgc tcggagccag ccggagcgcc
    48241 tcggcggcag cgcgcatcgc ctccgctttt gagccggttt cgagcctcgc ttgcgccagc
    48301 gcgagccgga tcagcggcga cttggcatcg agagtcgccg cggcctcgag ttcacgccgc
    48361 gccgctacgt gtttcttcgc gcggagaaga accagcccga gcgtgtgacg gatctcggcg
    48421 ttccgcggat acgagcgcga cagtgttgta aggatctcct cggccttcgg cgattgtccc
    48481 ccggctgcga ggttggcagc ctcggcgagc ctggcgcggg cggcgtccgg aagttgcgca
    48541 caaagcggca gtgcaagcag aaacagcgcg ctccggacgt tcacacgatc tattttgcca
    48601 gcaattcgcg gccgggtcga aaacgccact cccgaagcga agacgaactg gccggtgatc
    48661 ctgaccactt acaatatctt cggccgcaac cttccggcgg gagcgctcaa cccggacgga
    48721 caccacgtcc acgacgaaaa caaccttgct cccggacggc gggcagtttc gagtctattc
    48781 gccggttttt gggccaagca caaaagcatc cgcgacgacg gccagaaaat cggcccagtc
    48841 aatgccacgg ttgctgtctc ccatacggac gaggatgagt tgattgcgcg gcgagacgta
    48901 aagccgctgg ccgaagtgac cgttggcata gaagtcggac ggcgccggcg ccgggacctt
    48961 gcggccccac cattgatgtt taaaaaaacc gctgctccag acattcggcc cgggcggatc
    49021 cgagttcacg gacgttgatt cggagaccca cgccgccgga atcacctgtc gtgagttcca
    49081 gcgcccttgc tggaggtaga gtgcggcaaa cttggcgagg tcgatcgctc gcgccgtaag
    49141 accgctttcc cctttctcca agccgccgtg ctcgcgatcc acgatccaca tggcgtcgta
    49201 ctcggcgcct atcggctgcc agatgcgttc cgccaggtag ccggataatg tgggggcggc
    49261 agggtgccgg cggagcgcgc gctccagcgc ccagcccagg atgatgggac tcatatcctc
    49321 cgtgctgaag cgaacgccgg gctcaacatc gattccgacc cctcggacat aaccccgaac
    49381 atccgtggtg tagtacatcc ggggctgact cgtccagggc atgtttccgc gctcgtaatt
    49441 gattccggcc gtgttgtcga gcaagtgccg gagcgtaact cgcgtgaagc gaggatcctg
    49501 catctccgga atgtacttgg tgacgggatc gtcgatgccg ccgatccacc cgtccgcaat
    49561 cgccgcgccg ataagagccg agacgacgga cttggtcaca gagaaagact tgaacagcga
    49621 gtcgcggcga aacccgttga agtaacgctc gtccagaagg catccgtctt tgaccgcgat
    49681 gaatgcggtg gtcccgtgct ccgcgaggaa gcggcccaga gcagcttcat cggcgatcga
    49741 ttggtgcgaa tacgcaagct tcaacggcga gatccagtct ccagccgtac aatcagggaa
    49801 gcggaatggt tgcacgcttc ggggtaccgt ccgggacggc agcttgctgt aatcgtaaac
    49861 atccggcaag ttcaacaggg cccagcggcg gaaatagggg ctgaccgcca atgaggcgat
    49921 gaatattacg accaatccgg ccagaacacc gccggcgact cgaaccgcgc tccgaatcat
    49981 cggatacctc gctgatcttg acactgtcaa atcaagtgta gtagtataac ttgacgatgt
    50041 caagatcaaa acgcagcgaa gggagccgat ccgcggccga ggcgcagatt ccctatcaca
    50101 agggcaacct gcggcacaaa ctcctccgcg aggccgcccg gatgatcgcg aagagcggcg
    50161 tggaggccgt gtcgatgcgc aagctgagcg accggctcgg gatctcccgc acggccgcgt
    50221 atcactattt cgacaacaag ggcgaactgt tggccgccgt agggcagcat gggtttaccc
    50281 ggctggcgga tcgggttcag gcggcgggca agccggagct cccgccgctc gacaacctgc
    50341 ggcaggcgct cctggcctac gtacgcttcg ccacggagga gacggagttc ttcaggctca
    50401 tgttcgccaa cgttttgcag cgcccagtgc agatcggcgc taccgcagag ctaagcgcgt
    50461 tccgtttctc cagtcccgag gcatttgccg tctttgacgc acttttgtca gccgtcaagc
    50521 gttgtcaggc cgacaggtgc attcggggtg gagaccctct ggtggtggcg aatacgctgc
    50581 atgcgttcat ccacggcgtc gcccgcctcg cgatcgacga ccacctgaag attcagtgct
    50641 ccatggagga gttcttggat cagggcctgg acgcgctctt ctccggcttg gggggacctg
    50701 cgcaggcacg gttgccacag tgagggccag ccgggcatgg acggaccggt cgtgctccgg
    50761 ccatagagtt agggcgcctc gggcacgatg ggatgagttg cccctcgcgc cagcagcgcc
    50821 acgccgagcg caccaagcgg tatgctgaac cacaggatgc gtgtgtaggc caagacatcc
    50881 ggttcaacca tagcgaccgt gaggctctgc acccctacaa ccgcactgac cacgaagtga
    50941 agcatcaccg ctggccagat gctccggtcc aacagcacca acgctcccca ccaaatcgag
    51001 ataatccatg tttccaaggt caggagcaac gccgaaggaa gggagacccc gtgggtgaaa
    51061 acctgcgtga tgtgcaagac agcgaagagc acggacgcaa gaacaacact gaggatcatc
    51121 ccctgtttgg tgtttcccca aacgcgtacc agactataga ggacaaaccc tcggaataag
    51181 atttcttcgc tcagtccgac cgtgaactgg accacgactg tggtacgggc agcgggcaat
    51241 cgtacgaaat ttgaaatgtc gacggcgacc ttgccataga aggagtagag actcgcacag
    51301 gcaaagtaga tcaagcccgc caaggcaatc agccaaacct ggcgactccc cggtcggccg
    51361 acacccgatg ccttcagcca acccagacgc caaactagca gaaggacgca tacagttacg
    51421 gccaggcgac caaccgtggc ggtcgcagcg ccaccataag gctcggcaag cgcgctcgta
    51481 gcaatgcctg taaagaccaa caccaacaca aaccaggcaa ttgttgcact gacaacaaat
    51541 ccgatggggt ggttctctgc aaaggatcgg acgttattca tcagaagaac tcggacccgg
    51601 caaagcactc ttgatgactt actggtttct attggatggc cttgaaccag acaaactacc
    51661 ggcgagaatt ctaacgccgc cgtccgtaag agtcaaggaa attcagctcc gaaatgaacg
    51721 agctcgtggc accgtggcct gctatgctca attagggctg gccggccgct ccggtcggcg
    51781 aacctcgagc tggagagcaa gagatgtcgg ccgcttgcag atcaagctgg cgaaactgcg
    51841 ccgtggaagc cgattcggag atcctggaaa ttgatcggct ggaagatgaa atcgctcccg
    51901 ttacgtcgga cgtatcgagg gttcgagatc tcatctctgg tctcgagttg tgtcaccaca
    51961 aggctgagcg atgggtccac aacatcattg aggcgattag cctgggagag acaggaaagg
    52021 gtctgggcac gcgcccggca gtccagcttc accctgcgga acaagtttgg caaagcgctt
    52081 gtgacagtct ctcgggttgg tgcgggggat atccggccgc gtctaacgat ctgtgtgtcg
    52141 gcgccgttcc cgctattcga ctgcttgcgg gccttgggga ccgctccgcg ttgaaggagt
    52201 ggcaggtcca aagggtgatc gagaaagtcc gaagtctgat ccattggccg ctgtcgtcgg
    52261 atgacccggc agcacgatat gtgtacatcc tcctaggcgg cggcgagtac gaggccctct
    52321 atcgaaccca atgccccgaa cattacagga agcacgagga tttctggctt gctaccgttc
    52381 ggacgatgat tcacgacact gaaaatgggc gtgcggcggc actgtcgttg ggtctggcca
    52441 tcgatatgct ttggccatgc cactggaggt ttgtggagaa tcttcagatt gttctggacg
    52501 ccattggcgg gcggctgaag ccggacaagc cattcgccgc gtgcggcagg aacatatcgc
    52561 tgttaccgaa tcgtaaccgg atgttgaccg tttcaaggag cctaagagcc ttctgcggtc
    52621 actctgagac aggcggagaa gtggatcgag gaatactgtc cctgcttgga caacgcacag
    52681 cggcaaagag ctggctggcc gcctccctgg acaaaacaat ccgcctccag ttggacccgc
    52741 cagccggcgt gcgggcgatc tctgctttga ccggacctga ttggatcaca cggagcgtcg
    52801 gagcttaagt gtcccgcgag taacaccgca gtgaaagttt cgcgagagct accaggtgat
    52861 tccccctgag ggaggtggcc aggctcccag tatctgcctc agcacgacga tctgcccagc
    52921 gtggtagctg ttgtgtgacg cgattgcctg aagcattccc agtcggctat gcttcccccg
    52981 cgtggtcagg agatcagcct cgcgggactg gcggtccaac ttggccagtg cgttccgaaa
    53041 gcctcggacg gcccgctgcc attccgccgc gtttgccggg cccacgctgc cgggccagcc
    53101 gcccggcgca tgcttgggaa ccggagggtt ccctccgtca agccacttgg caacccactc
    53161 ctgccagtag ctcatgtggt ttagcagttg aaagaccgag tgtgggacac cctcgggccg
    53221 ggcgccggca gctttccaac ccagtcctgc gcaaagggtc ttggttgcca catgggagcc
    53281 ctttcccgac aaggcattgc cgattgtctt ctcgagcata ttgcggcgaa tggccatgaa
    53341 tccctcctct cgcaagagca ggtctcacgg acctcacgat accgcaattc gggaagctag
    53401 gcgagggcgg gatgggtgtg gtctccaagg ccacggacac ccgccccaac ccggtttcag
    53461 acggattgga cacgacccga tcacgcttcg tgtattggaa cagggtctag tgcggattgt
    53521 cggaagcagt cccgatcgta aaccggaact ggcgaggtca ccgacagggt gcctccctcg
    53581 gatggaaggt ggttactata tgcccaattc gatcacgaca aatcggacct gatgctggtg
    53641 gagaacttcc ggtacgaaac acggggtaca gtctgtccct cgccagtaag gtacgacgtt
    53701 ccggcgccag ccttcacttc accgccgatg ctcgttccga gagccgcgtc tgaatcagcc
    53761 gagcttggtg gggtgtaaag agaggaaact gaccttgagc cgcccgctga aacacgggaa
    53821 ggcagaatcc gtcgctcatg atgccggcgt ccttcgagtg gctgccgggg ccaagcagca
    53881 ggtgaccgac ttcgtgaacg agaacaatgc cgagaaccgt atgttccgga acccctctgc
    53941 ccacggcggt ggttcttgct ttctccgcga agacataggc gaccgacgga tggacggtct
    54001 cgccatagat cgagtggggg cgcccgagct gcttggcttg agcattccgt agcacgttga
    54061 taaaaagccc gccttgctct cgaatcgctg tgcaagtgaa acctcgggcc gcgtcttcgt
    54121 gcgggcgcgg gcacgccatc catttcacct tgacgccagc ccgcgacagg attctagcag
    54181 cgtcctcttc agcgcgggac agtacctcgt gaggcactcc tgcgtagtcg tacacgccga
    54241 cggtcaatgt cgcggactgg ccgtggagcg gctgcgtaac actccagcat cctgcgacaa
    54301 ggatgagtgc cgccggcact cggccggcgt tcgaacacac gtattgcgtc cagtttcggc
    54361 acatggttcc ctcccgaacc ctctagctgg actgtccgtt tctggcagtt acacgtgagc
    54421 cactgggaac ggggcagata tccaggccgc gttcccagtc gttgggtgtg tcatcacttc
    54481 ttgggcccgg aagggacgac gaggccaagc catctcatga tcaagtcgat cttcgttccc
    54541 aaagcgtcca ccttcgcctc tagttccgcg tccgcagggt caagcttctt catcgtgacg
    54601 atgcccccga tattcggagg aacagtcgtg aggccccacg ccaagcgctg gccgtcggca
    54661 aacacgtagc ccgtctcctg aaccacctcg ccagtggaag ggatccggaa gacacaattc
    54721 cctctgcagt ccgaggtcac cgtgcactgg acgtctaaga actgcgttac gtccatcaag
    54781 ggggcagcgc cgtctttgct cacttcgatg tgtccgaaat aggtagcttt tccttcctgg
    54841 tcaaaaatga cgatgccgct tcctgtttgc ggcatgccat tcggaacgag gttttcctgc
    54901 ggatagatgt aggaccacgc gcctgtcaga agctcctgct tgcactcgaa ctccgcgtcg
    54961 agtttcggcg gtgttgcaag gccgagcagc agcgcgactg ctaccggtaa agtgaagagc
    55021 tgcaatatcc tggttcgttt cattgtgatc gctctccttg aaatcgggtt catcataggc
    55081 attcccccgg ccggggctct ggagtacaat caggctcgga gacgcttagc cggataccgg
    55141 agcctgcgac cggcgagttt cagccgcaac ataccccgca accggcccgg aagtcccgaa
    55201 gaagttccga aagagttccg aagaagttat gaaagagcct gttccaaagt cggccaggca
    55261 tttctacagc tttggcgcgt ttcggctgta cgcgacggag cgggaactct ttcggggaag
    55321 caaaccggtt acgctgccgc cgaagacctg cgacacgctc ctgctgctgc tccgccggca
    55381 cggccacgtg gtggacaagg acgagctaat cagaacgctc tggccggaca ccttcgtcga
    55441 ggaggacaac ctggtgcagc aggtgtcgca cctgcggaag gcgctggggg agaggccgga
    55501 cggagggcct tacgtcgaga ctgttgcgag gcggggttat cgttttgccg cgctagtcga
    55561 ggagtcctgg gaggaaccgg cgattgcggg taggcgccgc gtgcccttac tgttctggac
    55621 tattgccgtc cctatcctga cggcgatcgc agcagcgatc cttctcctcc tgaacgtcgc
    55681 gggcctacga gaccggctgc tgcccaagcc aacgaatcca cgaatcacct ccatcgccgt
    55741 gttgccgctc gagaatctct cgcacgatcc cgagcaggaa tacttcgccg acggtctgac
    55801 cgaggaactg atcacaaccc ttggtaagat cagcgcgctg cgggtgattt cgcgaacgtc
    55861 ggtgatgcgg tacaaacgga cgaagaagcc ccttcccgaa atcgcccgcg aattgaatgt
    55921 ggacgccgtg atcgaaggca cgctgctgca atccggcggc cgcgtgcgca tcactgccaa
    55981 cctgctgcac gcgcctaccg atcggcacct atgggccgag acgtacgagc gcgacctggg
    56041 tgacatcctg acgttgcagc gggagctcgc ccgcaccgtc gccaaagaga ttcgcgcaac
    56101 gctttccccg gaggagcagg tgcaccttag ccgctcccgc cggctcaatc cggaggctca
    56161 cgagctttac ctgaagggtc aataccacta ctacaagtgg agaagcgcgg aatttgcaaa
    56221 ggcggttact tactttgaga aggcggccga ggccgatccc aattttgcac aggcttatct
    56281 cggactggcg aagacttacg ggtggcagtg gatgcagggt tctctgccgc ccaaagaggc
    56341 ctaccccaaa ttcagtgccg cgctaaagag ggcaatggag attgatgaca cgcttcccga
    56401 agcgcactat gtactggccg tgagtgcgtg gtatttctac tggaactgga cccaggcgga
    56461 agcggagttc aagcgcgcac tggcgctcaa tccgaacctg gaggaggctc gctttgaata
    56521 cggctggttc ttaacaacgc tcggtcgctt cacggaaggc atcgccgagg ccaggcgtgc
    56581 cgtcgagggc gatcccctgt cgttctcagc caatctggcc ttgggttcag tctatgtctt
    56641 cgcccgccag tacgacagag ccctggcaca gcatctcaag accatcgagc ttgatcccaa
    56701 cgatccccgc ggatacgagt tcctcgccgg tgcgtatgcg gctctggggc tgtacgatga
    56761 atcgctcaaa gcccgtgaaa agcagatgac gctggcaggt gccaagcccg aagacgtggc
    56821 cgcgatgggc gagtcatacc gatcttccgg atttgccggt tacttgcggt ggagactgga
    56881 gagggtaaag catccctacg agcgggcgat gatccaggcg caactgggac tgaaggacga
    56941 ggccttcgcg aacctggaaa agtgctaccg cgaacactgg tgggcaatgg ttcagctaaa
    57001 gtccctccca agttgggacc cgctgcgctc cgatccgcgt ttccaggacc tgctgcgccg
    57061 catgaatttt ccgccgtgag ccgtccgggt cggcgctcac cacagcagga gggcgtgtaa
    57121 ggccgaacgt tccgctacac gcgggtggcc atcggcgagg ccgatctaat gctggtggag
    57181 aacttccgct gagtgcttcg cagcgtacgc acttggcttc gacgagattc ctggtggaag
    57241 ccgaagtggg cgcagacact tcgagcaccg cctgcgcccg cttttttcta ctccagtccc
    57301 tcgcggctca ccaggaagcc atgcagggcg ccggaactct cgaagtaaaa cccgactatc
    57361 cacccgttgg cactgatccc aaacgggtac gtttcgactg cgtccgggta atcgaacaag
    57421 gtatagttcc cttcgctgag cagaaacccg tgcactatcc aatctggagt tgtgtagtcc
    57481 cccacgatct ggccacccgg gttaatcgca ttggcggacg tgccgatagc gcccgggaca
    57541 tcaatcgcgg tgaaggtgcc atcgtagcgg cgcaggaaac cgtgaaaggg tccgttgtac
    57601 ccgaactcgg agtagagccc cacgatgtca ccgtccggat tgatcccgcg ggcgttcgtg
    57661 agagtagcgc caagcggggt tgcatcaact gtcgtgaaga caccgtgact ccacagaaat
    57721 ccatgttcgc tgtagttctc gtagtccggc aggtcataag ccgcatagaa gcccacgatg
    57781 tcaccgtttt cattgatatc cattggaatg gtctcgcacg tcgttgcggg gcactggccg
    57841 ggaggaggat cgatccagat gaccttgcct tttctgagca ggaatccatg agaatagact
    57901 ggcaagaact tcgccgggtc tgatgtttgg cccacgacat cccctcgaga gttggtcccc
    57961 cgaacgtcca ctcttcggcc gaaggcggta tgggagatat cacggaatcc atccggagtg
    58021 aggagatacc cacgggtttg ccagcccgcg gggccccatt tgtaagtccc cacgaccgcg
    58081 cccgaggggg tgctatgccg ggcaagggtc cagacggctc cggggacgtc aatggatacg
    58141 aatgtccagg agtccgctgg cgtcgcaggc gtcacaccga acatcaagaa gaatgcaacc
    58201 atggccgtca cggagactga cggccaagcg tttgtcctct ttttcatcgc tagcctcctt
    58261 cccgccggtc tggctccgga gtacaatcaa gctcggagac gctgagccgg atggcggagc
    58321 atgcggccgg cgtggttcag ccgcaagata ccccgcatcc ggcccggaag tcccgaagaa
    58381 gtcccgaaga agttccgaaa aagttatgaa agagcctgtt tcaaagtcgg ccaggcattt
    58441 ctacagcttt ggcgcgttca ggctgtatgt gatggagcgg gaactctttc ggggaggcga
    58501 gccggttacg ctgcggccga agatcgacgt aagcggccac ggagttcagg ccgctctctc
    58561 acttccatgc tgctgaaggc gaactttcag gaggctgcgg agtcggctca ggagccccac
    58621 gggttcctgg agaagatgaa tggagtcctc caccggttcc ttcccagcgg catgttcgtg
    58681 gccgctacct ttctgtggtt cgagcacggg cagccggtgg tccggctgtc caacgccggg
    58741 ctcccgtatc cttatcgtgc ttcgaagcgg cgagcagagg ttagatgaaa tcccttctca
    58801 gggtgttcct ttgggtctgt tcgccggcga aggcctgata ccctatgaga ctcgcgaagt
    58861 acaccttggg aagcgcgatc tattaatggt tgcttctgac ggcttggggg acatcaggaa
    58921 tcgcagcggg gagttctttc aggattcaca actgcgccct acaccgaccg agcttagcgg
    58981 cagggatgtg gacagcttcc tggagcacct tgtccgcagc gcgacaacgt tcagcggcgg
    59041 ccagccgctg ttggatgata ttacagttgt agccgtgacg cgcacgtgaa aacaaggtgg
    59101 tgtaggactc ccgtcctgcg accgcacata ggagaggagg gcatggccgt gcatgtctgc
    59161 ccttggtgga tcggttactt actggcttgc ccgttgcgca ggtattcgca agatccggag
    59221 aagatcctgg gaccctatgt gcgcgagggt atgaccgtgc tcgagcccgg ccccggcatg
    59281 ggcttcttca ccctcgaggc cgcccgcagg gtaggcggtt ccggtcgcgt catcgcggtg
    59341 gagatacaac cgaagatgct ccaccggctc aagcgccgcg ctgagaaggc cggtcttcgc
    59401 gaacgtttgg atgcccggct ggcgatgccc gagtcgatga acctgaccgg cctggacggt
    59461 caagtggact ttgcgtttgc ctttgccgtc gtgcacgaaa tgcccggcac ggactcgttc
    59521 tttcttcaga ccgcccggtg tttgaagccg gacggctcac tgctgctggc tgagcctagg
    59581 ggtcacgtca aagccgaggg gttcgagaag gagttggcgg ctgccgcgcg agccggcttc
    59641 agtctggtcg aacgcccggt cattccacgc agccatgcag ccttgttgaa gaaaggctga
    59701 taagaggttc cggcgcaatc tctcaaccga ctaccacagc accggcacaa gccggctggg
    59761 cactcgctgc gcgtattccc gataaccttc caattcagcg cgcagggttc gatcttcgag
    59821 agacgtgcgg atcaccagca cagccgcggt aaacgctgct ggaatcacag cgagccacga
    59881 gccgagcgcg accggtgagg cgatcgtgaa cgcgagcgcg ccacagtagc ccggatgccg
    59941 cacatagcgg tacggcccgc cgtcaatcac ccgatggccg cgttcctcct ggatgcggac
    60001 cgtggccgag aagaaggggt tcgtcaccat tgcccaaaac gtgacgagac tacccagcgt
    60061 gcacacagcg aatgccgcgg cgctccaccc gatcgatacg gccggcggcc aatggacgcg
    60121 cacgtcccag gcggccacgc accagattgc tagaggtccg acaatggcca cgaccggtac
    60181 aaggaccttg tcccaaggct tggtgccctt ccgaattcgg gaccgctccg ccagcaagtc
    60241 cgggcttacc cggtggagta cgacaccaac aaccacctga attcccaacg tcaacgcgac
    60301 atagaaccag gcccgtaacc agtccgctcg ccccgaaacc agcctcagaa cagacgccag
    60361 aaccagtccc atgacgcagt acctgatgag cgagcttctg agcgaaggcg gacgcccggt
    60421 tggagacatg gtgtcagccg cgccgcgtgc gctgttcacg attccgacct cctgaatgat
    60481 tatcgcgaat cgtcggcgtc atctcgccgg cgcgtgtagc gagtaccgtt cttcccgtca
    60541 gtgtgtaatt ggaagcccgg agccgcagaa attggaggct ttaggctcaa tcgcagcttg
    60601 ctccgcgtgg atccgctccg ccagccgggc gcccatctgg tcagcctgcc gctggttaaa
    60661 gagcagtttg tgctgggctg cgtatgacag atcgtgtggg tcccattccc tgctcataat
    60721 gccggcggag gaatggccgc catcggccag taacagatgc ccgatttcgt gggcgaaaag
    60781 gtgccccaga tacaaaccct ggaagacctg cagccctttc gccagctctt ccacgcggcg
    60841 ccaaaagatc gaaacaacga ccccaaatcc cctggcaggc accagagaat aagcgcacgc
    60901 acccggagga agccccgcga gcttgattct cgcgacggga aggatccgga tgttgaggcg
    60961 cacggggccg ggagcctcct ggcactccgg atgctgatcc gaggagcagt cctgccaccg
    61021 gctggcaatt cccgcacggc ccaggattct ggcggcctcc cgctgagccg tcatgacgac
    61081 ttccagaggc gcgtcggcgt agttgtagat gcgaatgatg atttccgggg cgggttctgc
    61141 ccgcgtgccg ccggcgaaga gcgttctgtg cgaagccccg cccaacagcg ccgccaaagc
    61201 cgaattacag agcaaagagc ttctgactaa tcggttcagg gcgtttacct ccgcaggtcg
    61261 ttccgtgagg ccgagtctgc ggcaggtcag aagtccgctc aatggacggg ggcagatcac
    61321 gcaccgacgg caactgaaag gtttctgacc aactaacgag ctgatttcaa aaggcttaga
    61381 gagacagata agctgtgttg gaacgtctgg atagacgtaa gcgggcggca tgaactccga
    61441 gcgaccacta ggctatagat ccgggacgga tcatgtgggg cgcacatagc ctccttgctt
    61501 gactcctggg cgagccggac gcacaattcc ttgtagctct tggatgggac cgcagcacgg
    61561 atcctgaggg gcggcgggca tgagacagtc ctcccaatcg cattcggctt gccgcttcgg
    61621 cgcgtttgaa gtggacctgc gggcgcgtga actccgcaag gggggcctga agattaagct
    61681 gcaagagcag ccgtttcagg tgctggcgct gctgctggag cgggcgggcc aggtggtcac
    61741 ccgcgaggag ctgcggcagg cgctgtggcc ctccgacacc ttcgtggact tcgaccagag
    61801 cttgaacaca gcggtgcgga agctgcgcga ggtcctgggc gacacggcca gcacgccgcg
    61861 cttcatcgaa acgctgcccc ggaacgggta ccgcttcgtg gcgccgttgg aggcgatcgg
    61921 tgggaaggat ccggcgcgcg aaccgaaacg gaaaaagcag cggagattgg tttgggtcgc
    61981 ttcgttcgca gtcgcggtgg cattgggtgc gatcgctgcc tggtatttct ggcccaaggc
    62041 accggtgccg gaacgggtgc tgagtgcgat tccgctgacc agctacccgg gcgaggaaaa
    62101 ggaacctagt ttctcgccgg atggaggcca ggtggcattt gcatggaatg gggacaagga
    62161 ggacaaccag gacatctacg tcaagctgat cgggggcggc ccgccattgc gcctgaccac
    62221 tgatcctgcg gaggactacg gccccgcatg gtcgccggat ggccgccaca ttgctttcgt
    62281 tcgccggcgc cgggacagcg gagacctctt tctaattcca gctaacgggg gtccggagcg
    62341 ccggctggca gggatccagt ggtacgggct gcggatcaag ctgcgggatc ggcagttccc
    62401 cctcgtggcc tggtccccgg acagcaagtg ggtggcgttt gccaaccggg attcgccgga
    62461 acagccgtac tcattgttcc tgctgtcgcc cgagaccggc gagaagaggc agttgacttc
    62521 tcccccgaaa aagtcggtcg gggacggcgg tgtggccttc tctccggacg gacgacagtt
    62581 ggcattcgtt cgccttatgt cttcggggta cggccatctt tatgtgctcc cgctttccga
    62641 agagttccgg ccaaccggcg agccgcggcg tcttcccttc aatcagccgg tcaacatgac
    62701 tcccgcctgg acgccggacg gacgggagat cgttttcgcg gccggcccag ccggcagccg
    62761 gctgtggagg gtgaaggtgt caggctcgca gggaccgcaa ccgctggggt tgccgggaga
    62821 gtcagcctct ggacctgcgg tctctcggca aggcggtcgg cttgtgtacg tggcaggtac
    62881 gtcggacctg aatatctggc gcatggaact atccggcagg agcgagcccg gcccgccggt
    62941 gaggctgatt gcttccacca aggtggatgt ggctccccag ttctcgccgg acggcaagag
    63001 aatcgcattc gagtcggacc gtaccggcaa cggcgaggtc tgggtgtgcg atggtgacgg
    63061 tggcaaccag gcgcagatga cttcgttcgg ggcccacagt ggatcgcccc gatggtctcc
    63121 ggacggcgag cggatcgcct tcgactcgaa caaggaggga cggtggcaga tctacgtcgt
    63181 cgacgccgct gccggcgtcg cgagacgact gcatgacagt cctttcgacg acgattcgcc
    63241 gggttggtct catgacggga agtggatcta cttcgcgtcg aatcgaaccg gcgagttgca
    63301 ggtatggaag atgccggcga aagggggcga gccggtccag gtaacccgga gaggcggatt
    63361 cttagcctac gagtcccctg acgggaagta tctctactat atgaaaaggt tcgagccgat
    63421 ctcagaggta tggaaggtac cggtagattg tggggaggag atcaaggttc tcgatgctgt
    63481 ttggcgccgg ggtttcgccg taacgaatca ggggatctac ttcatgaccg tgccagaagc
    63541 cgatc
//
LOCUS       DATIFQ010000547         4361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_573640, whole genome shotgun sequence.
ACCESSION   DATIFQ010000547 DATIFQ010000000
VERSION     DATIFQ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573640"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..712
                     /locus_tag="VLE22_28150"
     CDS             95..712
                     /locus_tag="VLE22_28150"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HSB18357.1"
                     /translation="MKRVWVFLLLSGAAATAADWKPLFDGKDLTGWKMAGPGRFVVQN
                     GMLKTEGGMGLLYYEGEKFGNARLRVVFKTIGGSNSGVFIRLPEPPPDAWYGVHNGYE
                     VQILDGGDDWHCTGAIYSLSKALRGFQKPPGEWNTMEIALKGQKTTVFVNGKKVNEFV
                     GSQPVPERKMWYEPVRGPRPDFGYIGLQNHDDKSTVYFKEVSIKK"
     gene            complement(726..3245)
                     /locus_tag="VLE22_28155"
     CDS             complement(726..3245)
                     /locus_tag="VLE22_28155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18358.1"
                     /translation="MKISTLAVLLCASLPCSASAPQQLKVANEFVERVFEIRDGKVRT
                     TALVNKLDRRVYAMDSEEFELSVVWERIGYQHGDENPVRLMASDFTLGEFEQKAGPGG
                     EQQLIFPMTYRRFALEVRMVVSLGPEDAWVRKHLEIRATGRERVFLDRIIVESFTLRG
                     LEPRLGGFGQPLYADNVFFGIEYPASYNTAEGRKVRLSYYSGETTAPEPLRSERSVMG
                     VSPDGTVRASFLKYVGRIRSSKVRPVTVFNTWYDMQDNTLTEKNSNERMAALKTKLFD
                     PFSLKLDSFVLDDGWDDRNTVWAIHKDRFGGDLTSMRQSLEQNGTRLGIWFGPIGGYD
                     ERALRIAAGRKEGYEVTSNGEYFCLAGPKYRDKFRNVVMEMVGKYRVNHFKFDGLPYG
                     CNNPEHGHLLGVYSREAHLRAFIDLLQAIRAADPEIFLNITTSNWLSPWWLMYTDVVF
                     MGGFDYGFLNEVPAVSERQKGITYRDKVLYDDFRRYEYQFPNSSLMTIGIIKGLLGAE
                     GGASESLDDWTHNAIMNFSRGTMLTELYISPSMLSDPEWENLGRLMRWATDNSDVLLA
                     DTRFILGDPARAEVYGYSHFTKDRGIITLRNPTIEPRTVSVPLDRSSGVEPSFPRFQA
                     KVGYPYNEPLPGSFGYGDSLPFTVQGYGVVVLELAADDSAFKALSNGVRPDAEGKTAY
                     DPASAAVSGVRLLDLEVAGSSGAFTVAIPESTGSRRLALLCRPEGEGAQVSASLQLNG
                     QDLKFTVVSPQSTEKGLGYGQGRWTFLVAEAPSGQSRVDFRISQAGGAPFSGELQVWL
                     LADFYLAKRDLPAALQRPARAPLPATSEKEPRALHLFTRKM"
     gene            3367..3759
                     /locus_tag="VLE22_28160"
     CDS             3367..3759
                     /locus_tag="VLE22_28160"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSB18359.1"
                     /translation="MRYRLAGLIVGLVLVLAAQEKSEPRFEMTTYYLGLLRKGPSWSA
                     EVTDETKRIQAAHLAHIGKMADSGKLILAGPITDGGELRGIFVFKTDSIEEAKALSEA
                     DPAVEAGRLVVELHPWLGPKGLRYDPPK"
     gene            3837..>4361
                     /locus_tag="VLE22_28165"
     CDS             3837..>4361
                     /locus_tag="VLE22_28165"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSB18360.1"
                     /translation="MKIAVFGLGFMGSTHLKAIKNIPQAELVAVADTDEKRLSGDLSG
                     IQGNIGGPGEKLDFSKLRRYADWREAVKDPEVEAVDICFPTSFHADVTMGALRAGKHV
                     LVEKPMGLDGAAVDAMVAEAKRSGRILMSAQVLRFFPAYRVMADMVKTGKLGPVRSAI
                     FRRRCSAPAWSPWMA"
BASE COUNT          885 a         1276 c         1374 g          826 t
ORIGIN      
        1 atggaaaacc ggcggtttcc ggcgggaact ggtgcggttc gaagtgccgc ccgagtagac
       61 tgtgagagga atggttggag ggaagagaaa cgtcatgaaa cgggtttggg tatttctatt
      121 attgagtggc gccgcggcca ctgctgctga ctggaaacca ttgttcgacg ggaaggacct
      181 cacgggttgg aaaatggcag gccccggtcg cttcgtggta cagaatggca tgctcaagac
      241 agagggcggt atgggcctgc tgtattacga gggcgagaag ttcggcaatg caaggctccg
      301 cgttgtattc aagacaatcg gcggctcgaa ctcgggcgtc ttcattcggc ttcccgagcc
      361 cccgcccgat gcttggtacg gagttcacaa cggctacgag gtgcagattc tggatggcgg
      421 cgatgactgg cactgcaccg gggcgatcta ctccctcagc aaggcgctgc gcggctttca
      481 gaaaccaccg ggcgaatgga acaccatgga gattgctcta aagggtcaga aaaccaccgt
      541 cttcgtaaat ggcaagaagg tcaacgagtt tgtcggcagc cagcccgtgc cggagcgaaa
      601 gatgtggtac gagcccgtac gcggcccccg gccagacttc ggctacatcg gcctgcaaaa
      661 ccacgacgat aagtccacgg tgtacttcaa ggaggtcagc atcaagaagt agccgccggc
      721 ccgagctaca tcttccgcgt gaacaggtgt aaagcccggg gctctttctc ggaagtcgca
      781 ggaaggggcg cgcgagccgg gcgctgtaac gctgcgggca ggtccctctt cgccaggtag
      841 aaatcagcca gcagccacac ctgaagttcg ccggagaagg gcgctccgcc cgcctgggaa
      901 atccggaaat ccacgcggct ttggcccgat ggcgcctcgg cgaccaggaa tgtccaacgg
      961 ccctgtccgt atcccagccc cttttccgtc gattgcgggc tcaccacggt gaacttaaga
     1021 tcctgcccgt tcaattgaag cgacgccgag acctgggctc cctctccctc cgggcggcac
     1081 aggagggcta ggcgccggct ccccgtgctc tcgggaatcg ccaccgtaaa ggcgccggag
     1141 gaaccggcga cctcgagatc caaaaggcgg acgccggaga ccgcggccga ggccgggtcg
     1201 taagcggtct tgccttcagc gtcgggccgg acaccgttgg agagcgcctt gaacgccgag
     1261 tcatcagcgg ctagttcgag gaccacgacg ccgtagcctt gcacggtgaa cggaagagag
     1321 tccccgtagc cgaacgagcc cggtaacggt tcgttgtacg ggtagccgac cttcgcctgg
     1381 aagcgcggga atgacggctc gactcccgaa ctgcggtcga gagggaccga aactgtccgc
     1441 ggttcgatcg tcgggttgcg caaagtgatg atgccgcgat ccttcgtgaa gtgcgagtag
     1501 ccgtagacct cggcccgggc cgggtctccc agaatgaagc gagtgtcggc aagaagcacg
     1561 tcgctgttat cggtggccca gcgcatcagc ctcccgaggt tctcccattc cggatccgac
     1621 agcatggacg gagagatgta caactcggta agcatcgtac cccggctgaa gttcatgatc
     1681 gcgttgtgcg tccagtcgtc gaggctctca ctcgcgccgc cctcggcccc gagcagtccc
     1741 ttgatgatgc cgatcgtcat cagagaagag ttcgggaact ggtattcgta gcggcggaag
     1801 tcgtcgtaca gcaccttgtc gcggtaggta atccctttct ggcgctcgct gacagccggc
     1861 acttcattca agaagccgta gtcgaatccg cccataaaga cgacgtccgt atacatgagc
     1921 caccacggac tcagccagtt gctcgtggtg atgttaagga aaatctccgg gtccgcggcg
     1981 cggatcgcct ggagcaaatc gataaacgca cgcaggtgcg cttcgcggga gtagacgccg
     2041 aggaggtggc cgtgttccgg attattgcag ccgtaaggaa ggccgtcgaa cttgaagtga
     2101 ttgacgcggt acttgcctac catctccatc accacgttgc gaaacttgtc acggtacttc
     2161 gggcccgcca gacagaagta ttctccgttc gaggtgacct cgtagccctc tttccgaccg
     2221 gcggcgatgc gcagcgcgcg ctcgtcgtag ccgccgatcg gcccaaacca gatacccagg
     2281 cgtgtgccgt tttgttcgag cgactgccgc attgaagtga ggtcgccgcc gaagcggtcc
     2341 ttgtggatcg cccacacggt gttgcggtcg tcccagccgt cgtccaggac aaacgaatcg
     2401 agtttcagag agaaagggtc gaagagtttg gtcttcagtg cggccatacg ctcgttagag
     2461 ttcttctcgg tcagagtgtt gtcctgcatg tcgtaccaag tattgaaaac cgtcaccggc
     2521 cgaaccttac tgcttcggat gcggccgaca tatttcagga aggacgcgcg aaccgtgccg
     2581 tccggcgaca ctcccatgac cgatcgctcg ctccgcaagg gttcgggtgc agtggtttcg
     2641 ccggagtagt acgaaaggcg gactttgcgg ccctcggcgg tgttgtaaga cgccgggtac
     2701 tcgatgccga agaacacgtt gtcggcatag agcggctggc cgaaaccacc gagccgcggc
     2761 tcgaggccac gcagcgtgaa gctctccacg atgatccggt ccaggaagac ccgctctctg
     2821 ccggtggcgc ggatttcaag atgcttgcgg acccaggcat cctccggtcc gaggcttacc
     2881 accatacgca cctcaagagc gaaccgccgg tacgtcattg ggaagatcag ttgttgttcg
     2941 ccgccgggtc ccgctttttg ctcgaactca cccagcgtaa aatcggacgc catcaagcgg
     3001 accgggttct catcgccgtg ctggtagccg atacgctccc agacaaccga caactcaaac
     3061 tcctcggaat ccatggcgta gacacggcga tcgagcttgt tgaccagcgc ggttgtgcgg
     3121 accttgccat cgcgaatctc gaagacgcgt tcgacgaact cgttggctac tttcaactgt
     3181 tgcggggcgg aggccgagca gggcagggaa gcgcacagca gtactgccag agtagagatc
     3241 ttcatggtga cgactcgtga aacctcacag gcagcatatg gcttgtcacc ctttcggcgc
     3301 aacgtccgcc cgtctgcgtt gcgatatact tcgtcaacga cacctcgact ctgggaggaa
     3361 gcggttatga gatatcgcct ggcaggcctg atcgttggac ttgtgttggt gcttgcggca
     3421 caagaaaagt ctgagccgcg attcgaaatg actacgtact acctgggtct gcttcgcaaa
     3481 gggccctcgt ggagcgccga ggtcactgac gaaacgaagc gcatccaggc cgcgcacctt
     3541 gcgcacatcg gcaagatggc ggattccggc aagctgatcc tcgccggtcc gattaccgat
     3601 ggcggcgagt tgcgcggcat cttcgtgttc aagactgact ccatcgagga ggcgaaggcg
     3661 ctgtccgaag cagacccggc cgtcgaggcg ggccgtcttg tcgtcgagtt acacccatgg
     3721 ctcggcccga agggacttcg ttacgacccg ccgaaatgat aacggcctgc ggttccgcgc
     3781 ggagccgcga tcccaactga caccgcccgc tgcccgctga taccatattc ctgactatga
     3841 aaatcgcagt ctttggctta ggtttcatgg gtagcaccca tctcaaggcg attaaaaaca
     3901 ttcctcaggc agaattggtc gcagtggcgg acacggacga aaaacgccta agcggtgatc
     3961 tcagtggaat ccagggcaac attggagggc cgggagagaa gctcgacttc tcgaagcttc
     4021 gccgctacgc cgactggcga gaggcggtga aagatccgga agtcgaggcg gtcgacatct
     4081 gcttccctac cagtttccat gctgacgtga ccatgggcgc acttcgcgcc ggcaaacacg
     4141 tgctggtcga gaagcccatg gggctcgacg gcgcggctgt ggacgcgatg gttgccgagg
     4201 caaagcgcag cgggcgaatt ctgatgagcg cgcaggtgct tcgcttcttc ccggcgtacc
     4261 gggtgatggc ggacatggtg aagaccggta agctcgggcc ggtgcgttcc gcgatttttc
     4321 ggcggcggtg ctcggcgccg gcgtggagtc catggatggc t
//
LOCUS       DATIFQ010000548         2888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_957683, whole genome shotgun sequence.
ACCESSION   DATIFQ010000548 DATIFQ010000000
VERSION     DATIFQ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957683"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /gene="dnaN"
                     /locus_tag="VLE22_28170"
     CDS             complement(<1..597)
                     /gene="dnaN"
                     /locus_tag="VLE22_28170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HSB18361.1"
                     /translation="MWVTCAQTALLKGVQLASRAISTRTTMPILGYILLETQKNGVKL
                     TATDLELAVQVEIDAEVKRGGQITLPARILSEIVGNLPEATVELKSAESGPQVQITCE
                     ASDFEILGLPASDFPTQPKADGNEVGALEADVLRRLVSQTIFAVSSDETRPFLTGVYI
                     ALEGPEVRFVATDGGRLALRKTMLASPAKQKTGVIVPAK"
     gene            complement(780..2114)
                     /gene="dnaA"
                     /locus_tag="VLE22_28175"
     CDS             complement(780..2114)
                     /gene="dnaA"
                     /locus_tag="VLE22_28175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HSB18362.1"
                     /translation="MATETTAASGVWQEALRRIEGRLTKPSYESFVKAMAPVSLQGDV
                     FVFSVPSRLAKEWVETRYAGLIAGALREVLARSISIQVTVAEHAAPTPPPPVPARLPD
                     TVQLSPKYTFDTFVIGSGNRFAHAAAMAVAESPARAYNPLFIYGGVGLGKTHLLQAIG
                     HHVIHGKGLRRVAYISSEKFTNELINAIRDDRTLEFRTRYRNVDVLLIDDIQFLAGKE
                     RTQEEFFHTFNTLHEASRQIIITSDRPPKDIPTLEDRLRSRFEWGLIADIQPPDLETR
                     IAILRKKAELDGIRVPDEVAEFIAQRIQSNIRELEGALVRVVAYANLTRSPITGELAS
                     EILKELLPAAAAKPVTIASLQKIVAEHFDIRVEEMRAKRRTKGVAFPRQVAMYLAREL
                     TDASLPRIGEEFGGRDHTTVMHACDRVKAAIAADAQFAARLKRLVDTLRTTM"
     gene            complement(2145..>2888)
                     /locus_tag="VLE22_28180"
     CDS             complement(2145..>2888)
                     /locus_tag="VLE22_28180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSB18363.1"
                     /translation="GPEVRLAVYDSPLVAPRVISLHGEEFHAFVGDLAARTYEHSRER
                     GGRIPFIVIREIVENLIHAYFEGAVISIFDDGNTIRISDQGPGIADPAKAIQPGFTTA
                     TAEMRSFIKGVGSGLPVAREQLAFLGGAISIDSNLSRGTVVTLSLTRTVTPSRPTGAA
                     AVDPPITPPVDRRPPDLTARQKKVLLLIAELGSAGPSAVAKELAVSQSTAYRELQMLA
                     DLHLIQSRHAGKRTLTENGIAALGTVFKP"
BASE COUNT          513 a          877 c          937 g          561 t
ORIGIN      
        1 cttggcgggt acaatcaccc cggtcttctg tttggcgggc gacgccagca tggtcttgcg
       61 cagcgccaga cggccgccgt ccgtggccac gaaccgcact tccggcccct ccaaggcgat
      121 gtagactccg gtgagaaacg gccgggtttc atcggatgag acggcaaaga tggtttgact
      181 gaccagccgg cggagcacgt cggcttcgag cgcgccgacc tcattcccgt cggctttggg
      241 ctgcgtggga aaatcgctgg ccggcaaacc gaggatctcg aaatcactcg cttcacacgt
      301 gatctgcacc tgcggaccgc tctccgcact cttcagttcc accgttgctt ccgggaggtt
      361 cccaacaatc tcagagagga tccgcgccgg cagcgtgatc tgtccccccc gtttgacctc
      421 agcatcaatt tccacttgca cagccagttc cagatcggtt gctgtgagct tcaccccgtt
      481 cttttgcgtt tccagcagaa tgtatcccaa gatcggcatc gtcgttcggg tagagatggc
      541 acggctggcc agctgtacgc ctttgagcag tgcagtctgc gcgcaggtca cccacatcgc
      601 cggttatcct ccaagataac tatatataaa cttcgtagta atagtagtaa tgctgtggat
      661 agtgtggatg gatgggcacc aggcccacga tagcaagggg aatagggtgt gtatagggct
      721 ctggataact caacgcgatc atccgcagtg tccaccgtcg tgcttgcgcc tggacgggct
      781 cacatagttg tccgcaacgt gtcaacaagc cgcttcaacc gcgcggcgaa ctgagcgtcc
      841 gccgcgatag cagctttgac gcgatcgcac gcgtgcatga cggtggtatg gtcgcggccg
      901 ccgaactctt caccgatacg gggcagggag gcgtcggtga gctcgcgggc gagatacatc
      961 gcgacctggc gcgggaacgc gacgcctttg gtccggcgtt tcgcgcgcat ctcttcgacg
     1021 cgaatgtcga agtgttccgc cacgatcttc tgcagggacg cgatcgtcac cggtttggcc
     1081 gcggccgccg gaagcagttc tttgaggatc tcgctggcca gctcaccagt gatgggagaa
     1141 cgtgtgaggt tcgcgtaggc gaccacacgg acgagggcgc cttccagctc ccggatgttg
     1201 gactggatgc gctgcgcaat gaactcggcg acctcgtcgg gcacacggat cccgtccaac
     1261 tcggccttct tgcgcaagat ggcaatgcgt gtctccaggt ccgggggctg gatatcggcg
     1321 atcaggcccc attcgaaccg ggagcgcagg cggtcttcca gcgtcgggat gtctttcggc
     1381 ggtcggtcgc tggtgatgat gatctgcctg ctggcttcgt gcagcgtatt gaaggtatgg
     1441 aaaaactctt cctgtgttcg ctccttcccg gccagaaact ggatgtcgtc gatcaacagc
     1501 acgtccacgt tgcgataccg cgtacggaac tcgagggtgc ggtcatcccg gatggcattg
     1561 atgagttcgt tggtaaactt ctcgctgctg atgtaagcga ccctgcgcag gcccttgccg
     1621 tggatgacgt ggtggccgat ggcctgcagc agatgcgtct tgcccagccc cacgcccccg
     1681 tagatgaaga gcggattgta tgcgcgggcc ggagattccg ccacggccat ggctgcggcg
     1741 tgggcgaagc ggttgccgga cccgatcacg aacgtgtcaa aagtgtactt ggggctcaac
     1801 tgcacggtgt ccgggaggcg ggcagggacc gggggcggtg gggtcggcgc ggcatgctcc
     1861 gcgacggtga cctggatgga gatgctccgg gcaagcacct ctcggagcgc gccggcgatc
     1921 agtcccgcat accgggtctc cacccattct ttggccagcc ggctgggaac ggagaagacg
     1981 aagacgtcgc cctgaagact gaccggggcc atggccttga cgaatgattc ataactcggt
     2041 ttggtcagtc ggccttcgat ccgccgcagc gcttcctgcc agacgccgct ggcggcggtg
     2101 gtctcggtag ccatcggatg agtcctcctg ccccggaacg acctctacgg tttgaacacc
     2161 gtgccgagcg cggcgatacc attctcagtc aacgtccgtt taccggcgtg ccggctttgg
     2221 atcagatgca gatcagccaa catctgcagt tcgcggtacg cggtgctctg actgacggcg
     2281 agttctttgg cgacggcaga gggccccgct gagccgagct cggcgatgag caacaggacc
     2341 ttcttctgcc gcgccgtcag gtcgggcggg cggcgatcca ccggtggggt gatcggcggg
     2401 tccacggccg ccgcccccgt cggtcgcgac ggagtgaccg tgcgtgtcag cgacagggtg
     2461 acgaccgtgc cgcgcgagag gttgctgtcg atggagatgg caccgcccag gaaggcgagc
     2521 tgctcgcggg cgacaggcaa ccccgacccc acacctttga tgaaggagcg catctccgct
     2581 gtcgcggtcg tgaatcccgg ctgtatcgcc ttggcaggat cggcgatgcc cggcccctga
     2641 tcggagatcc gaatggtgtt cccgtcgtcg aagatgctga tcacggcgcc ttcgaaatag
     2701 gcgtggatga ggttctccac gatctctctg atgacgatga acgggatccg gccgccccgc
     2761 tcacgggaat gctcatacgt ccgcgccgca agatcgccga caaacgcatg aaactcctcg
     2821 ccgtggaggc tgatcacacg gggggcgacg agaggcgaat cgtagacggc gagccggact
     2881 tccggccc
//
LOCUS       DATIFQ010000549         4358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bryobacteraceae bacterium isolate SMAG_U16399
            k141_2489915, whole genome shotgun sequence.
ACCESSION   DATIFQ010000549 DATIFQ010000000
VERSION     DATIFQ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935893
            Sequence Read Archive: SRR25158319
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacteraceae bacterium (soil metagenome)
  ORGANISM  Bryobacteraceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales;
            Bryobacteraceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:02:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,640
            CDSs (total)                      :: 5,584
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 4 of 20
            Pseudo Genes (incomplete)         :: 17 of 20
            Pseudo Genes (internal stop)      :: 3 of 20
            Pseudo Genes (multiple problems)  :: 4 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Bryobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489915"
                     /isolate="SMAG_U16399"
                     /isolation_source="soil"
                     /db_xref="taxon:2212468"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VLE22_28185"
     CDS             <1..936
                     /locus_tag="VLE22_28185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSB18364.1"
                     /translation="EYGGKWYKGTYGWNFTIFDGEIEQIAHRNTFDAGMWPGFGNAYL
                     LTGDPAYIGTLRRQMDNLYAQKKVVDGQLMIPHMYGDPRGHGQSGQEAWYQWTPNLFT
                     NRLTEIYFWSGARNDLERVPKADWTAFLEGQNPGYPEEALRAELLAIRAKVEEMRNDP
                     TTPDTRLADYLMGFNPAQTDALTKLTLGGYLTGNIWTLHSRVRYFDPVRRRSGLPEDV
                     GALVEKIGSDAVTLTLVNTNPVDARTVIVQAGAYGEHQFISVASGGKTVPVNGPLVTV
                     RLEPGCGARLEIRMERYHNQPTLAQPWSRGWMVEP"
     gene            933..2195
                     /locus_tag="VLE22_28190"
     CDS             933..2195
                     /locus_tag="VLE22_28190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSB18365.1"
                     /translation="MSTARDMRWPVVGLLSLGMMISYFDRVNLTVALPSMAAGFGLDK
                     AQQGLALSAVFWAYAALQIPGGALLDRYGVRIPYLVGFLLWSLATASTALTTGLGSLI
                     ALRLVVGAGEAVVAPASMRYISLHFSEKQRGLAVGLYMTGTKLGPALGLPLAAGLVAG
                     IGWQWMFLVLGVASLLWLVPWLLWVKRDDPAALPRTSAPAAQRQRLPVKKMITSPVIW
                     GILLGTYCYMYFVYYSMTWMPIYLKERHGMSLQEMGWYGGVSFAGMAIVAALSGWAAD
                     WLIGRGGDPVNVRKAFTIAGFAAASTQTMCAVVSSTTVMLFFVVFSLCGLGLATANYW
                     ALTQTLIPSGSIATVVGIQNSAASLAGIVAPWLTGWMIQQTGSFDAPIKGVGLWLVIG
                     IGCYVFLVRRKYAFRAVSQAEKARVATP"
     gene            complement(2162..3268)
                     /gene="menC"
                     /locus_tag="VLE22_28195"
     CDS             complement(2162..3268)
                     /gene="menC"
                     /locus_tag="VLE22_28195"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HSB18366.1"
                     /translation="MKIDRIDLTHVRIPLVEPFRISSGAVSEKDALVIRLSSEGLTGW
                     GESSPMAGSFYSSDTPESCWRELCGTLLPSLAGRQFESLDDAAQCVAAIRASNFAKAG
                     VETALWDLEAQRQSKPLYRLLGGTRSLAESGLAVGLYDDVKDMFRAIERYLTDGYKRV
                     KIKIEPGRDIELIRAVRRTFGEIPLFVDANGAYGLRHTGIFQTLDEFNLMMYEQPFPG
                     PMLAELADLQRQVRTPVCLDESLESEADLRRAIELGSFRIANIKIQRVGGLRPALRIY
                     EICREHNIPVWVGTMPELGIGCAQGAALATLDHCRFPTDVEASLRWFTDDLTDPLLTV
                     EDGLLNLPEAPGLGYRVDERKLERYGVATRAFSA"
     gene            complement(3275..>4358)
                     /locus_tag="VLE22_28200"
     CDS             complement(3275..>4358)
                     /locus_tag="VLE22_28200"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HSB18367.1"
                     /translation="NFRRTILRQGEPSHISIVEFGVHQDVKAAFLGRPVRTLRDEVEF
                     WVRAGYDFVPIHTGIRRFIWPGSSFQDERAPQAAESLLLRKGTAAYSVYTGEDRSLTW
                     AEESRGRITNLEEFERFPWPDPDAMDLSAFEEAKQYLPPGMKVIAYMGWVFTSVWTLM
                     GMETFCLALGEQPELIRRMFDRVWSIQSRILMRILQFDSVGAVTHPDDLAYAEALTIS
                     PEHYRKYVFPWYRWNCAMVRESGRFNMYHSDGKLDTVLEDIIGCGFDAMHPIEPKAMD
                     IVEVKRKVAGRITLIGNIDLGYTLTRGTPAEVDAEVKERIRTVGPGGGYCVGSSNSIP
                     SYVPLANYNAMREAAFRYGHYPLKLD"
BASE COUNT          764 a         1225 c         1549 g          820 t
ORIGIN      
        1 gaatacggcg gcaagtggta caagggcacc tacggctgga acttcaccat tttcgacggc
       61 gagatcgaac agatcgcaca ccgcaacacc tttgacgcgg ggatgtggcc gggcttcggc
      121 aacgcctacc tgttgacggg cgatccggcc tatatcggca cgctgcggcg ccagatggac
      181 aacctttacg cacagaagaa ggtcgtggac gggcagctca tgattccgca catgtacggc
      241 gatccgcgcg gccacggaca gagtgggcag gaggcctggt atcagtggac accgaacctg
      301 ttcacgaacc ggttgacgga gatctacttc tggtccggag cccgcaatga tctcgagcgc
      361 gtgccgaagg cggattggac cgcgttcctc gagggacaga atccaggcta cccggaagag
      421 gcgctacgcg cggagctgct agcgatccgg gcgaaggtcg aagagatgcg caacgatccg
      481 accacgcccg acacgcgact ggccgactat ctgatggggt tcaaccccgc ccagaccgac
      541 gcgctcacaa agttgacgct cggcggctac cttacaggca acatctggac gctgcacagc
      601 cgggtgcgct acttcgatcc agtgcggcgg cgttccgggc tgccggagga tgtgggagca
      661 ctggtcgaga agatcggctc ggacgctgtc acgctaacgc tggtgaatac gaacccggtg
      721 gacgcccgaa cggtgatcgt gcaggcgggc gcgtacgggg agcatcagtt tatttccgtg
      781 gcatctgggg gaaagacggt gccggtgaac ggaccgctgg tgaccgtccg gttagagccg
      841 ggatgtggcg cgaggctgga gattcggatg gaacggtatc acaaccagcc aacgctggcg
      901 cagccgtgga gccgtggctg gatggtggag ccgtgagcac cgcacgagac atgcgctggc
      961 cggtggtggg gctgctgagc ctcgggatga tgatctcgta ctttgaccgt gtgaacctga
     1021 ccgtcgcgct gccatcgatg gcggccgggt ttggtctcga caaggcgcag caggggctgg
     1081 cgctatcggc agtgttctgg gcatacgcgg cgcttcagat tccgggaggc gcgctgctcg
     1141 accgctacgg cgtgcgcatc ccgtacctgg tcggctttct gttgtggagc ctcgccaccg
     1201 cgagtacggc gctgaccacc gggctgggga gcctgatcgc gcttcggctg gtggtgggcg
     1261 cgggggaggc ggtggtggct ccggcgagca tgcgctacat cagcctgcat ttttccgaga
     1321 agcagcgcgg gctggcggtg gggctgtaca tgacgggcac gaaactcggt ccggcgctcg
     1381 ggctgccgct ggccgcgggg ctggtggccg gcatcggctg gcagtggatg tttctggtgc
     1441 tgggcgtggc gagcctgctg tggctggttc cgtggctcct ctgggtgaag cgggacgacc
     1501 cggctgctct accccgaacg agcgcgccgg cagcccagag gcagagactg cccgtgaaga
     1561 agatgatcac gagtccggtg atctggggaa ttcttctcgg gacttactgc tatatgtact
     1621 tcgtgtacta ctcgatgacg tggatgccca tctatctgaa ggaacggcat ggcatgtcgc
     1681 tgcaggagat gggttggtac ggaggggtgt cttttgcagg catggcgatc gtggcggccc
     1741 tctctggttg ggcggcggac tggctgatcg ggaggggcgg ggacccggtg aacgtgcgca
     1801 aggcgttcac gatcgcaggt ttcgcggcag catcaacgca aacgatgtgc gccgtggttt
     1861 catccacgac ggtgatgctg ttcttcgtgg tgttctcgct gtgcggcctg ggccttgcaa
     1921 cggcgaacta ctgggcgctg acgcagaccc tgattccgag cggcagtatc gcgacagtgg
     1981 tgggaatcca aaacagcgcg gcgagcctgg cggggatcgt cgccccatgg ctgactggat
     2041 ggatgatcca gcagacgggc agtttcgatg cgccgatcaa gggcgttggg ttgtggcttg
     2101 tgatcgggat cgggtgttat gtgtttctgg tgcggcggaa gtacgcattc agggcggtgt
     2161 ctcaggcgga gaaggctcgc gtggcgacgc cgtagcgttc gagtttccgc tcatccacgc
     2221 gatagccgag tccgggtgct tcagggaggt tgaggagtcc gtcttcgacc gtcagtagcg
     2281 ggtcggtcaa atcatcggtg aaccagcgca ggctggcctc gacatccgtc gggaagcggc
     2341 agtggtcgag cgtggccagg gcagcgccct gcgcgcaccc gattccgagc tctggcatcg
     2401 tccccaccca gaccgggatg ttgtgctccc ggcagatttc gtaaatgcga agggcggggc
     2461 gaaggccgcc gacgcgctgt atcttgatgt tggcgatacg gaagctgccc aactcgatgg
     2521 cgcgccgcag gtcggcttcg gactccaggc tttcatcgag gcagaccggc gtgcggacct
     2581 gccgttggag atcggcgagc tcggcgagca tcgggcccgg gaacggctgc tcgtacatca
     2641 tgaggttgaa ctcgtcgagc gtctggaaga ttcccgtgtg ccgcaggccg tacgcgccgt
     2701 tagcatctac gaacagggga atctcgccga acgtgcggcg gacagctcga atcagctcga
     2761 tgtcgcggcc cggttcgatc ttgatcttaa cccgcttgta gccgtcggtg agatatcgct
     2821 cgatggcgcg aaacatgtcc ttgacgtcgt cgtacaggcc gaccgcgagg ccggactcgg
     2881 ccaaggagcg cgtgccaccg agcagcctgt acagcggctt gctctgccgt tgcgcctcga
     2941 gatcccacag cgcggtttcg acgcccgcct tggcgaagtt gctcgcgcgg atggcggcga
     3001 cgcactgagc ggcgtcgtcg agcgattcga actggcgtcc agcgagcgat ggcaacagcg
     3061 ttccgcagag ttcgcgccag cacgattccg gcgtgtcact cgagtagaag ctgccggcca
     3121 tgggcgagga ttcgccccag ccggtgagtc cctcggagga gaggcggatc acgagggcgt
     3181 ccttctcgct aaccgcgccg gagctgatgc ggaaaggctc gacgagcggg atgcgaacgt
     3241 gcgtgaggtc gatgcggtcg attttcatgc ggctttagtc caacttcaaa ggatagtgac
     3301 cgtaacggaa agccgcctcg cgcatggcgt tgtagtttgc gagcgggacg tacgagggga
     3361 tggagttgct tgaacccacg cagtagccgc cgccggggcc gacggttcgg atacgttcct
     3421 tcacctcggc gtcgacctcg gccggcgtgc cgcgcgtgag agtgtagccg aggtcgatgt
     3481 tcccaatcag cgtgatgcgt ccggcaactt tgcgtttgac ctcgacgatg tccatggctt
     3541 tcggctcgat cgggtgcatg gcgtcaaaac cgcaaccgat gatgtcttcg agcacggtgt
     3601 cgagtttccc gtcggagtgg tacatgttga accggccgct ttcacggacc atcgcgcaat
     3661 tccagcggta ccaggggaag acgtacttgc ggtaatgctc tggggagatg gtgagcgctt
     3721 cggcgtaggc gaggtcgtcg ggatgggtga cggcgccgac cgagtcgaat tggagaatgc
     3781 gcatgaggat acggctctgg atcgaccaga cgcggtcgaa catgcggcgg atcaattcgg
     3841 gttgttcgcc gagggcgagg cagaaggtct ccatgcccat gagcgtccac accgaggtga
     3901 agacccatcc catatacgcg atgaccttca tccccggcgg gagatactgc ttggcttctt
     3961 cgaacgcgga gaggtccatg gcgtcggggt cgggccacgg gaagcgctcg aattcttcga
     4021 ggtttgtgat cctcccacgg ctttcttcgg cccacgtgag gctgcggtcc tcgccggtgt
     4081 agacgctgta ggcggctgtt cctttgcgca ggagaaggga ttcggccgct tgcggcgcgc
     4141 gttcgtcctg gaaggaggaa ccgggccaga tgaagcggcg aattccggta tgaatgggaa
     4201 cgaagtcgta gccggcgcgt acccagaatt ccacctcgtc gcgcagggta cggacggggc
     4261 ggccgaggaa ggcggccttc acgtcctgat ggacaccgaa ctcgacgatc gagatgtggc
     4321 tcggttcgcc ctggcggaga atcgtacgcc ggaagttt
//
